                         SEQUENCE LISTING

<110>  CureVac AG, Sanofi Pasteur SA
 
<120>  Zika virus vaccine

<130>  CU01P207WO1

<160>  11759 

<170>  PatentIn version 3.5

<210>  1
<211>  3423
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein

<400>  1

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
    130                 135                 140                 


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
        435                 440                 445             


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
    450                 455                 460                 


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
465                 470                 475                 480 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
                485                 490                 495     


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
            500                 505                 510         


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
        515                 520                 525             


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
    530                 535                 540                 


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
545                 550                 555                 560 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
            580                 585                 590         


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
        595                 600                 605             


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
    610                 615                 620                 


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
625                 630                 635                 640 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
                645                 650                 655     


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
        675                 680                 685             


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
    690                 695                 700                 


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
                725                 730                 735     


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
        755                 760                 765             


Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
    770                 775                 780                 


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
785                 790                 795                 800 


Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr 
                805                 810                 815     


Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser 
            820                 825                 830         


Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile 
        835                 840                 845             


Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser 
    850                 855                 860                 


Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu 
865                 870                 875                 880 


Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln 
                885                 890                 895     


Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp 
            900                 905                 910         


Gly Lys Ser His Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val 
        915                 920                 925             


Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp 
    930                 935                 940                 


Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser 
945                 950                 955                 960 


Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala 
                965                 970                 975     


Val Ile Gly Thr Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu 
            980                 985                 990         


Gly Tyr Trp Ile Glu Ser Glu Lys  Asn Asp Thr Trp Arg  Leu Lys Arg 
        995                 1000                 1005             


Ala His  Leu Ile Glu Met Lys  Thr Cys Glu Trp Pro  Lys Ser His 
    1010                 1015                 1020             


Thr Leu  Trp Thr Asp Gly Ile  Glu Glu Ser Asp Leu  Ile Ile Pro 
    1025                 1030                 1035             


Lys Ser  Leu Ala Gly Pro Leu  Ser His His Asn Thr  Arg Glu Gly 
    1040                 1045                 1050             


Tyr Arg  Thr Gln Met Lys Gly  Pro Trp His Ser Glu  Glu Leu Glu 
    1055                 1060                 1065             


Ile Arg  Phe Glu Glu Cys Pro  Gly Thr Lys Val His  Val Glu Glu 
    1070                 1075                 1080             


Thr Cys  Gly Thr Arg Gly Pro  Ser Leu Arg Ser Thr  Thr Ala Ser 
    1085                 1090                 1095             


Gly Arg  Val Ile Glu Glu Trp  Cys Cys Arg Glu Cys  Thr Met Pro 
    1100                 1105                 1110             


Pro Leu  Ser Phe Arg Ala Lys  Asp Gly Cys Trp Tyr  Gly Met Glu 
    1115                 1120                 1125             


Ile Arg  Pro Arg Lys Glu Pro  Glu Ser Asn Leu Val  Arg Ser Met 
    1130                 1135                 1140             


Val Thr  Ala Gly Ser Thr Asp  His Met Asp His Phe  Ser Leu Gly 
    1145                 1150                 1155             


Val Leu  Val Ile Leu Leu Met  Val Gln Glu Gly Leu  Lys Lys Arg 
    1160                 1165                 1170             


Met Thr  Thr Lys Ile Ile Ile  Ser Thr Ser Met Ala  Val Leu Val 
    1175                 1180                 1185             


Ala Met  Ile Leu Gly Gly Phe  Ser Met Ser Asp Leu  Ala Lys Leu 
    1190                 1195                 1200             


Ala Ile  Leu Met Gly Ala Thr  Phe Ala Glu Met Asn  Thr Gly Gly 
    1205                 1210                 1215             


Asp Val  Ala His Leu Ala Leu  Ile Ala Ala Phe Lys  Val Arg Pro 
    1220                 1225                 1230             


Ala Leu  Leu Val Ser Phe Ile  Phe Arg Ala Asn Trp  Thr Pro Arg 
    1235                 1240                 1245             


Glu Ser  Met Leu Leu Ala Leu  Ala Ser Cys Leu Leu  Gln Thr Ala 
    1250                 1255                 1260             


Ile Ser  Ala Leu Glu Gly Asp  Leu Met Val Leu Ile  Asn Gly Phe 
    1265                 1270                 1275             


Ala Leu  Ala Trp Leu Ala Ile  Arg Ala Met Val Val  Pro Arg Thr 
    1280                 1285                 1290             


Asp Asn  Ile Thr Leu Ala Ile  Leu Ala Ala Leu Thr  Pro Leu Ala 
    1295                 1300                 1305             


Arg Gly  Thr Leu Leu Val Ala  Trp Arg Ala Gly Leu  Ala Thr Cys 
    1310                 1315                 1320             


Gly Gly  Phe Met Leu Leu Ser  Leu Lys Gly Lys Gly  Ser Val Lys 
    1325                 1330                 1335             


Lys Asn  Leu Pro Phe Val Met  Ala Leu Gly Leu Thr  Ala Val Arg 
    1340                 1345                 1350             


Leu Val  Asp Pro Ile Asn Val  Val Gly Leu Leu Leu  Leu Thr Arg 
    1355                 1360                 1365             


Ser Gly  Lys Arg Ser Trp Pro  Pro Ser Glu Val Leu  Thr Ala Val 
    1370                 1375                 1380             


Gly Leu  Ile Cys Ala Leu Ala  Gly Gly Phe Ala Lys  Ala Asp Ile 
    1385                 1390                 1395             


Glu Met  Ala Gly Pro Met Ala  Ala Val Gly Leu Leu  Ile Val Ser 
    1400                 1405                 1410             


Tyr Val  Val Ser Gly Lys Ser  Val Asp Met Tyr Ile  Glu Arg Ala 
    1415                 1420                 1425             


Gly Asp  Ile Thr Trp Glu Lys  Asp Ala Glu Val Thr  Gly Asn Ser 
    1430                 1435                 1440             


Pro Arg  Leu Asp Val Ala Leu  Asp Glu Ser Gly Asp  Phe Ser Leu 
    1445                 1450                 1455             


Val Glu  Asp Asp Gly Pro Pro  Met Arg Glu Ile Ile  Leu Lys Val 
    1460                 1465                 1470             


Val Leu  Met Thr Ile Cys Gly  Met Asn Pro Ile Ala  Ile Pro Phe 
    1475                 1480                 1485             


Ala Ala  Gly Ala Trp Tyr Val  Tyr Val Lys Thr Gly  Lys Arg Ser 
    1490                 1495                 1500             


Gly Ala  Leu Trp Asp Val Pro  Ala Pro Lys Glu Val  Lys Lys Gly 
    1505                 1510                 1515             


Glu Thr  Thr Asp Gly Val Tyr  Arg Val Met Thr Arg  Arg Leu Leu 
    1520                 1525                 1530             


Gly Ser  Thr Gln Val Gly Val  Gly Val Met Gln Glu  Gly Val Phe 
    1535                 1540                 1545             


His Thr  Met Trp His Val Thr  Lys Gly Ser Ala Leu  Arg Ser Gly 
    1550                 1555                 1560             


Glu Gly  Arg Leu Asp Pro Tyr  Trp Gly Asp Val Lys  Gln Asp Leu 
    1565                 1570                 1575             


Val Ser  Tyr Cys Gly Pro Trp  Lys Leu Asp Ala Ala  Trp Asp Gly 
    1580                 1585                 1590             


His Ser  Glu Val Gln Leu Leu  Ala Val Pro Pro Gly  Glu Arg Ala 
    1595                 1600                 1605             


Arg Asn  Ile Gln Thr Leu Pro  Gly Ile Phe Lys Thr  Lys Asp Gly 
    1610                 1615                 1620             


Asp Ile  Gly Ala Val Ala Leu  Asp Tyr Pro Ala Gly  Thr Ser Gly 
    1625                 1630                 1635             


Ser Pro  Ile Leu Asp Lys Cys  Gly Arg Val Ile Gly  Leu Tyr Gly 
    1640                 1645                 1650             


Asn Gly  Val Val Ile Lys Asn  Gly Ser Tyr Val Ser  Ala Ile Thr 
    1655                 1660                 1665             


Gln Gly  Arg Arg Glu Glu Glu  Thr Pro Val Glu Cys  Phe Glu Pro 
    1670                 1675                 1680             


Ser Met  Leu Lys Lys Lys Gln  Leu Thr Val Leu Asp  Leu His Pro 
    1685                 1690                 1695             


Gly Ala  Gly Lys Thr Arg Arg  Val Leu Pro Glu Ile  Val Arg Glu 
    1700                 1705                 1710             


Ala Ile  Lys Thr Arg Leu Arg  Thr Val Ile Leu Ala  Pro Thr Arg 
    1715                 1720                 1725             


Val Val  Ala Ala Glu Met Glu  Glu Ala Leu Arg Gly  Leu Pro Val 
    1730                 1735                 1740             


Arg Tyr  Met Thr Thr Ala Val  Asn Val Thr His Ser  Gly Thr Glu 
    1745                 1750                 1755             


Ile Val  Asp Leu Met Cys His  Ala Thr Phe Thr Ser  Arg Leu Leu 
    1760                 1765                 1770             


Gln Pro  Ile Arg Val Pro Asn  Tyr Asn Leu Tyr Ile  Met Asp Glu 
    1775                 1780                 1785             


Ala His  Phe Thr Asp Pro Ser  Ser Ile Ala Ala Arg  Gly Tyr Ile 
    1790                 1795                 1800             


Ser Thr  Arg Val Glu Met Gly  Glu Ala Ala Ala Ile  Phe Met Thr 
    1805                 1810                 1815             


Ala Thr  Pro Pro Gly Thr Arg  Asp Ala Phe Pro Asp  Ser Asn Ser 
    1820                 1825                 1830             


Pro Ile  Met Asp Thr Glu Val  Glu Val Pro Glu Arg  Ala Trp Ser 
    1835                 1840                 1845             


Ser Gly  Phe Asp Trp Val Thr  Asp Tyr Ser Gly Lys  Thr Val Trp 
    1850                 1855                 1860             


Phe Val  Pro Ser Val Arg Asn  Gly Asn Glu Ile Ala  Ala Cys Leu 
    1865                 1870                 1875             


Thr Lys  Ala Gly Lys Arg Val  Ile Gln Leu Ser Arg  Lys Thr Phe 
    1880                 1885                 1890             


Glu Thr  Glu Phe Gln Lys Thr  Lys His Gln Glu Trp  Asp Phe Val 
    1895                 1900                 1905             


Val Thr  Thr Asp Ile Ser Glu  Met Gly Ala Asn Phe  Lys Ala Asp 
    1910                 1915                 1920             


Arg Val  Ile Asp Ser Arg Arg  Cys Leu Lys Pro Val  Ile Leu Asp 
    1925                 1930                 1935             


Gly Glu  Arg Val Ile Leu Ala  Gly Pro Met Pro Val  Thr His Ala 
    1940                 1945                 1950             


Ser Ala  Ala Gln Arg Arg Gly  Arg Ile Gly Arg Asn  Pro Asn Lys 
    1955                 1960                 1965             


Pro Gly  Asp Glu Tyr Leu Tyr  Gly Gly Gly Cys Ala  Glu Thr Asp 
    1970                 1975                 1980             


Glu Asp  His Ala His Trp Leu  Glu Ala Arg Met Leu  Leu Asp Asn 
    1985                 1990                 1995             


Ile Tyr  Leu Gln Asp Gly Leu  Ile Ala Ser Leu Tyr  Arg Pro Glu 
    2000                 2005                 2010             


Ala Asp  Lys Val Ala Ala Ile  Glu Gly Glu Phe Lys  Leu Arg Thr 
    2015                 2020                 2025             


Glu Gln  Arg Lys Thr Phe Val  Glu Leu Met Lys Arg  Gly Asp Leu 
    2030                 2035                 2040             


Pro Val  Trp Leu Ala Tyr Gln  Val Ala Ser Ala Gly  Ile Thr Tyr 
    2045                 2050                 2055             


Thr Asp  Arg Arg Trp Cys Phe  Asp Gly Thr Thr Asn  Asn Thr Ile 
    2060                 2065                 2070             


Met Glu  Asp Ser Val Pro Ala  Glu Val Trp Thr Arg  His Gly Glu 
    2075                 2080                 2085             


Lys Arg  Val Leu Lys Pro Arg  Trp Met Asp Ala Arg  Val Cys Ser 
    2090                 2095                 2100             


Asp His  Ala Ala Leu Lys Ser  Phe Lys Glu Phe Ala  Ala Gly Lys 
    2105                 2110                 2115             


Arg Gly  Ala Ala Phe Gly Val  Met Glu Ala Leu Gly  Thr Leu Pro 
    2120                 2125                 2130             


Gly His  Met Thr Glu Arg Phe  Gln Glu Ala Ile Asp  Asn Leu Ala 
    2135                 2140                 2145             


Val Leu  Met Arg Ala Glu Thr  Gly Ser Arg Pro Tyr  Lys Ala Ala 
    2150                 2155                 2160             


Ala Ala  Gln Leu Pro Glu Thr  Leu Glu Thr Ile Met  Leu Leu Gly 
    2165                 2170                 2175             


Leu Leu  Gly Thr Val Ser Leu  Gly Ile Phe Phe Val  Leu Met Arg 
    2180                 2185                 2190             


Asn Lys  Gly Ile Gly Lys Met  Gly Phe Gly Met Val  Thr Leu Gly 
    2195                 2200                 2205             


Ala Ser  Ala Trp Leu Met Trp  Leu Ser Glu Ile Glu  Pro Ala Arg 
    2210                 2215                 2220             


Ile Ala  Cys Val Leu Ile Val  Val Phe Leu Leu Leu  Val Val Leu 
    2225                 2230                 2235             


Ile Pro  Glu Pro Glu Lys Gln  Arg Ser Pro Gln Asp  Asn Gln Met 
    2240                 2245                 2250             


Ala Ile  Ile Ile Met Val Ala  Val Gly Leu Leu Gly  Leu Ile Thr 
    2255                 2260                 2265             


Ala Asn  Glu Leu Gly Trp Leu  Glu Arg Thr Lys Ser  Asp Leu Ser 
    2270                 2275                 2280             


His Leu  Met Gly Arg Arg Glu  Glu Gly Ala Thr Met  Gly Phe Ser 
    2285                 2290                 2295             


Met Asp  Ile Asp Leu Arg Pro  Ala Ser Ala Trp Ala  Ile Tyr Ala 
    2300                 2305                 2310             


Ala Leu  Thr Thr Phe Ile Thr  Pro Ala Val Gln His  Ala Val Thr 
    2315                 2320                 2325             


Thr Ser  Tyr Asn Asn Tyr Ser  Leu Met Ala Met Ala  Thr Gln Ala 
    2330                 2335                 2340             


Gly Val  Leu Phe Gly Met Gly  Lys Gly Met Pro Phe  Tyr Ala Trp 
    2345                 2350                 2355             


Asp Phe  Gly Val Pro Leu Leu  Met Ile Gly Cys Tyr  Ser Gln Leu 
    2360                 2365                 2370             


Thr Pro  Leu Thr Leu Ile Val  Ala Ile Ile Leu Leu  Val Ala His 
    2375                 2380                 2385             


Tyr Met  Tyr Leu Ile Pro Gly  Leu Gln Ala Ala Ala  Ala Arg Ala 
    2390                 2395                 2400             


Ala Gln  Lys Arg Thr Ala Ala  Gly Ile Met Lys Asn  Pro Val Val 
    2405                 2410                 2415             


Asp Gly  Ile Val Val Thr Asp  Ile Asp Thr Met Thr  Ile Asp Pro 
    2420                 2425                 2430             


Gln Val  Glu Lys Lys Met Gly  Gln Val Leu Leu Met  Ala Val Ala 
    2435                 2440                 2445             


Val Ser  Ser Ala Ile Leu Ser  Arg Thr Ala Trp Gly  Trp Gly Glu 
    2450                 2455                 2460             


Ala Gly  Ala Leu Ile Thr Ala  Ala Thr Ser Thr Leu  Trp Glu Gly 
    2465                 2470                 2475             


Ser Pro  Asn Lys Tyr Trp Asn  Ser Ser Thr Ala Thr  Ser Leu Cys 
    2480                 2485                 2490             


Asn Ile  Phe Arg Gly Ser Tyr  Leu Ala Gly Ala Ser  Leu Ile Tyr 
    2495                 2500                 2505             


Thr Val  Thr Arg Asn Ala Gly  Leu Val Lys Arg Arg  Gly Gly Gly 
    2510                 2515                 2520             


Thr Gly  Glu Thr Leu Gly Glu  Lys Trp Lys Ala Arg  Leu Asn Gln 
    2525                 2530                 2535             


Met Ser  Ala Leu Glu Phe Tyr  Ser Tyr Lys Lys Ser  Gly Ile Thr 
    2540                 2545                 2550             


Glu Val  Cys Arg Glu Glu Ala  Arg Arg Ala Leu Lys  Asp Gly Val 
    2555                 2560                 2565             


Ala Thr  Gly Gly His Ala Val  Ser Arg Gly Ser Ala  Lys Leu Arg 
    2570                 2575                 2580             


Trp Leu  Val Glu Arg Gly Tyr  Leu Gln Pro Tyr Gly  Lys Val Ile 
    2585                 2590                 2595             


Asp Leu  Gly Cys Gly Arg Gly  Gly Trp Ser Tyr Tyr  Ala Ala Thr 
    2600                 2605                 2610             


Ile Arg  Lys Val Gln Glu Val  Lys Gly Tyr Thr Lys  Gly Gly Pro 
    2615                 2620                 2625             


Gly His  Glu Glu Pro Val Leu  Val Gln Ser Tyr Gly  Trp Asn Ile 
    2630                 2635                 2640             


Val Arg  Leu Lys Ser Gly Val  Asp Val Phe His Met  Ala Ala Glu 
    2645                 2650                 2655             


Pro Cys  Asp Thr Leu Leu Cys  Asp Ile Gly Glu Ser  Ser Ser Ser 
    2660                 2665                 2670             


Pro Glu  Val Glu Glu Ala Arg  Thr Leu Arg Val Leu  Ser Met Val 
    2675                 2680                 2685             


Gly Asp  Trp Leu Glu Lys Arg  Pro Gly Ala Phe Cys  Ile Lys Val 
    2690                 2695                 2700             


Leu Cys  Pro Tyr Thr Ser Thr  Met Met Glu Thr Leu  Glu Arg Leu 
    2705                 2710                 2715             


Gln Arg  Arg Tyr Gly Gly Gly  Leu Val Arg Val Pro  Leu Ser Arg 
    2720                 2725                 2730             


Asn Ser  Thr His Glu Met Tyr  Trp Val Ser Gly Ala  Lys Ser Asn 
    2735                 2740                 2745             


Thr Ile  Lys Ser Val Ser Thr  Thr Ser Gln Leu Leu  Leu Gly Arg 
    2750                 2755                 2760             


Met Asp  Gly Pro Arg Arg Pro  Val Lys Tyr Glu Glu  Asp Val Asn 
    2765                 2770                 2775             


Leu Gly  Ser Gly Thr Arg Ala  Val Val Ser Cys Ala  Glu Ala Pro 
    2780                 2785                 2790             


Asn Met  Lys Ile Ile Gly Asn  Arg Ile Glu Arg Ile  Arg Ser Glu 
    2795                 2800                 2805             


His Ala  Glu Thr Trp Phe Phe  Asp Glu Asn His Pro  Tyr Arg Thr 
    2810                 2815                 2820             


Trp Ala  Tyr His Gly Ser Tyr  Glu Ala Pro Thr Gln  Gly Ser Ala 
    2825                 2830                 2835             


Ser Ser  Leu Ile Asn Gly Val  Val Arg Leu Leu Ser  Lys Pro Trp 
    2840                 2845                 2850             


Asp Val  Val Thr Gly Val Thr  Gly Ile Ala Met Thr  Asp Thr Thr 
    2855                 2860                 2865             


Pro Tyr  Gly Gln Gln Arg Val  Phe Lys Glu Lys Val  Asp Thr Arg 
    2870                 2875                 2880             


Val Pro  Asp Pro Gln Glu Gly  Thr Arg Gln Val Met  Ser Met Val 
    2885                 2890                 2895             


Ser Ser  Trp Leu Trp Lys Glu  Leu Gly Lys His Lys  Arg Pro Arg 
    2900                 2905                 2910             


Val Cys  Thr Lys Glu Glu Phe  Ile Asn Lys Val Arg  Ser Asn Ala 
    2915                 2920                 2925             


Ala Leu  Gly Ala Ile Phe Glu  Glu Glu Lys Glu Trp  Lys Thr Ala 
    2930                 2935                 2940             


Val Glu  Ala Val Asn Asp Pro  Arg Phe Trp Ala Leu  Val Asp Lys 
    2945                 2950                 2955             


Glu Arg  Glu His His Leu Arg  Gly Glu Cys Gln Ser  Cys Val Tyr 
    2960                 2965                 2970             


Asn Met  Met Gly Lys Arg Glu  Lys Lys Gln Gly Glu  Phe Gly Lys 
    2975                 2980                 2985             


Ala Lys  Gly Ser Arg Ala Ile  Trp Tyr Met Trp Leu  Gly Ala Arg 
    2990                 2995                 3000             


Phe Leu  Glu Phe Glu Ala Leu  Gly Phe Leu Asn Glu  Asp His Trp 
    3005                 3010                 3015             


Met Gly  Arg Glu Asn Ser Gly  Gly Gly Val Glu Gly  Leu Gly Leu 
    3020                 3025                 3030             


Gln Arg  Leu Gly Tyr Val Leu  Glu Glu Met Ser Arg  Ile Pro Gly 
    3035                 3040                 3045             


Gly Arg  Met Tyr Ala Asp Asp  Thr Ala Gly Trp Asp  Thr Arg Ile 
    3050                 3055                 3060             


Ser Arg  Phe Asp Leu Glu Asn  Glu Ala Leu Ile Thr  Asn Gln Met 
    3065                 3070                 3075             


Glu Lys  Gly His Arg Ala Leu  Ala Leu Ala Ile Ile  Lys Tyr Thr 
    3080                 3085                 3090             


Tyr Gln  Asn Lys Val Val Lys  Val Leu Arg Pro Ala  Glu Lys Gly 
    3095                 3100                 3105             


Lys Thr  Val Met Asp Ile Ile  Ser Arg Gln Asp Gln  Arg Gly Ser 
    3110                 3115                 3120             


Gly Gln  Val Val Thr Tyr Ala  Leu Asn Thr Phe Thr  Asn Leu Val 
    3125                 3130                 3135             


Val Gln  Leu Ile Arg Asn Met  Glu Ala Glu Glu Val  Leu Glu Met 
    3140                 3145                 3150             


Gln Asp  Leu Trp Leu Leu Arg  Arg Ser Glu Lys Val  Thr Asn Trp 
    3155                 3160                 3165             


Leu Gln  Ser Asn Gly Trp Asp  Arg Leu Lys Arg Met  Ala Val Ser 
    3170                 3175                 3180             


Gly Asp  Asp Cys Val Val Lys  Pro Ile Asp Asp Arg  Phe Ala His 
    3185                 3190                 3195             


Ala Leu  Arg Phe Leu Asn Asp  Met Gly Lys Val Arg  Lys Asp Thr 
    3200                 3205                 3210             


Gln Glu  Trp Lys Pro Ser Thr  Gly Trp Asp Asn Trp  Glu Glu Val 
    3215                 3220                 3225             


Pro Phe  Cys Ser His His Phe  Asn Lys Leu His Leu  Lys Asp Gly 
    3230                 3235                 3240             


Arg Ser  Ile Val Val Pro Cys  Arg His Gln Asp Glu  Leu Ile Gly 
    3245                 3250                 3255             


Arg Ala  Arg Val Ser Pro Gly  Ala Gly Trp Ser Ile  Arg Glu Thr 
    3260                 3265                 3270             


Ala Cys  Leu Ala Lys Ser Tyr  Ala Gln Met Trp Gln  Leu Leu Tyr 
    3275                 3280                 3285             


Phe His  Arg Arg Asp Leu Arg  Leu Met Ala Asn Ala  Ile Cys Ser 
    3290                 3295                 3300             


Ser Val  Pro Val Asp Trp Val  Pro Thr Gly Arg Thr  Thr Trp Ser 
    3305                 3310                 3315             


Ile His  Gly Lys Gly Glu Trp  Met Thr Thr Glu Asp  Met Leu Val 
    3320                 3325                 3330             


Val Trp  Asn Arg Val Trp Ile  Glu Glu Asn Asp His  Met Glu Asp 
    3335                 3340                 3345             


Lys Thr  Pro Val Thr Lys Trp  Thr Asp Ile Pro Tyr  Leu Gly Lys 
    3350                 3355                 3360             


Arg Glu  Asp Leu Trp Cys Gly  Ser Leu Ile Gly His  Arg Pro Arg 
    3365                 3370                 3375             


Thr Thr  Trp Ala Glu Asn Ile  Lys Asn Thr Val Asn  Met Val Arg 
    3380                 3385                 3390             


Arg Ile  Ile Gly Asp Glu Glu  Lys Tyr Met Asp Tyr  Leu Ser Thr 
    3395                 3400                 3405             


Gln Val  Arg Tyr Leu Gly Glu  Glu Gly Ser Thr Pro  Gly Val Leu 
    3410                 3415                 3420             


<210>  2
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C

<400>  2

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg 
            100                 


<210>  3
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-ER anchor

<400>  3

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
        115                 120         


<210>  4
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) pr

<400>  4

Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
            20                  25                  30          


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
                85                  90              


<210>  5
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) M

<400>  5

Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
1               5                   10                  15      


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
            20                  25                  30          


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
        35                  40                  45              


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
    50                  55                  60                  


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
65                  70                  75  


<210>  6
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prM

<400>  6

Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
            20                  25                  30          


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
                85                  90                  95      


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
            100                 105                 110         


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
        115                 120                 125             


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
    130                 135                 140                 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
145                 150                 155                 160 


Leu Leu Ile Ala Pro Ala Tyr Ser 
                165             


<210>  7
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) E

<400>  7

Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
1               5                   10                  15      


Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr 
            20                  25                  30          


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
        35                  40                  45              


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
    50                  55                  60                  


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
65                  70                  75                  80  


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
                85                  90                  95      


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
            100                 105                 110         


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
        115                 120                 125             


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
    130                 135                 140                 


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
145                 150                 155                 160 


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
                165                 170                 175     


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
            180                 185                 190         


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
        195                 200                 205             


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
    210                 215                 220                 


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
225                 230                 235                 240 


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
                245                 250                 255     


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
            260                 265                 270         


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
        275                 280                 285             


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
    290                 295                 300                 


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
305                 310                 315                 320 


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
                325                 330                 335     


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
            340                 345                 350         


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
        355                 360                 365             


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
    370                 375                 380                 


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
385                 390                 395                 400 


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
                405                 410                 415     


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
            420                 425                 430         


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
        435                 440                 445             


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
    450                 455                 460                 


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
465                 470                 475                 480 


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
                485                 490                 495     


Phe Leu Ser Thr Ala Val Ser Ala 
            500                 


<210>  8
<211>  672
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME

<400>  8

Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
            20                  25                  30          


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
                85                  90                  95      


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
            100                 105                 110         


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
        115                 120                 125             


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
    130                 135                 140                 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
145                 150                 155                 160 


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
                165                 170                 175     


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val 
            180                 185                 190         


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
        195                 200                 205             


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
    210                 215                 220                 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
225                 230                 235                 240 


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
                245                 250                 255     


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
            260                 265                 270         


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
        275                 280                 285             


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
    290                 295                 300                 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
305                 310                 315                 320 


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
                325                 330                 335     


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
            340                 345                 350         


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
        355                 360                 365             


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
    370                 375                 380                 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
385                 390                 395                 400 


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
                405                 410                 415     


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
            420                 425                 430         


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
        435                 440                 445             


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
    450                 455                 460                 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
465                 470                 475                 480 


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
                485                 490                 495     


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
            500                 505                 510         


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
        515                 520                 525             


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
    530                 535                 540                 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
545                 550                 555                 560 


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
                565                 570                 575     


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
            580                 585                 590         


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
        595                 600                 605             


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
    610                 615                 620                 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
625                 630                 635                 640 


Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
                645                 650                 655     


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
            660                 665                 670         


<210>  9
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS1

<400>  9

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu 
    50                  55                  60                  


Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu 
65                  70                  75                  80  


Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro 
                85                  90                  95      


Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro 
            100                 105                 110         


His Gly Trp Lys Ala Trp Gly Lys Ser His Phe Val Arg Ala Ala Lys 
        115                 120                 125             


Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro 
    130                 135                 140                 


Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe 
145                 150                 155                 160 


Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser 
                165                 170                 175     


Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Lys Glu 
            180                 185                 190         


Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp 
        195                 200                 205             


Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr 
                245                 250                 255     


Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu 
            260                 265                 270         


Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu 
        275                 280                 285             


Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 
    290                 295                 300                 


Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr Ala 
            340                 345                 350         


<210>  10
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS2A

<400>  10

Gly Ser Thr Asp His Met Asp His Phe Ser Leu Gly Val Leu Val Ile 
1               5                   10                  15      


Leu Leu Met Val Gln Glu Gly Leu Lys Lys Arg Met Thr Thr Lys Ile 
            20                  25                  30          


Ile Ile Ser Thr Ser Met Ala Val Leu Val Ala Met Ile Leu Gly Gly 
        35                  40                  45              


Phe Ser Met Ser Asp Leu Ala Lys Leu Ala Ile Leu Met Gly Ala Thr 
    50                  55                  60                  


Phe Ala Glu Met Asn Thr Gly Gly Asp Val Ala His Leu Ala Leu Ile 
65                  70                  75                  80  


Ala Ala Phe Lys Val Arg Pro Ala Leu Leu Val Ser Phe Ile Phe Arg 
                85                  90                  95      


Ala Asn Trp Thr Pro Arg Glu Ser Met Leu Leu Ala Leu Ala Ser Cys 
            100                 105                 110         


Leu Leu Gln Thr Ala Ile Ser Ala Leu Glu Gly Asp Leu Met Val Leu 
        115                 120                 125             


Ile Asn Gly Phe Ala Leu Ala Trp Leu Ala Ile Arg Ala Met Val Val 
    130                 135                 140                 


Pro Arg Thr Asp Asn Ile Thr Leu Ala Ile Leu Ala Ala Leu Thr Pro 
145                 150                 155                 160 


Leu Ala Arg Gly Thr Leu Leu Val Ala Trp Arg Ala Gly Leu Ala Thr 
                165                 170                 175     


Cys Gly Gly Phe Met Leu Leu Ser Leu Lys Gly Lys Gly Ser Val Lys 
            180                 185                 190         


Lys Asn Leu Pro Phe Val Met Ala Leu Gly Leu Thr Ala Val Arg Leu 
        195                 200                 205             


Val Asp Pro Ile Asn Val Val Gly Leu Leu Leu Leu Thr Arg Ser Gly 
    210                 215                 220                 


Lys Arg 
225     


<210>  11
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS2B

<400>  11

Ser Trp Pro Pro Ser Glu Val Leu Thr Ala Val Gly Leu Ile Cys Ala 
1               5                   10                  15      


Leu Ala Gly Gly Phe Ala Lys Ala Asp Ile Glu Met Ala Gly Pro Met 
            20                  25                  30          


Ala Ala Val Gly Leu Leu Ile Val Ser Tyr Val Val Ser Gly Lys Ser 
        35                  40                  45              


Val Asp Met Tyr Ile Glu Arg Ala Gly Asp Ile Thr Trp Glu Lys Asp 
    50                  55                  60                  


Ala Glu Val Thr Gly Asn Ser Pro Arg Leu Asp Val Ala Leu Asp Glu 
65                  70                  75                  80  


Ser Gly Asp Phe Ser Leu Val Glu Asp Asp Gly Pro Pro Met Arg Glu 
                85                  90                  95      


Ile Ile Leu Lys Val Val Leu Met Thr Ile Cys Gly Met Asn Pro Ile 
            100                 105                 110         


Ala Ile Pro Phe Ala Ala Gly Ala Trp Tyr Val Tyr Val Lys Thr Gly 
        115                 120                 125             


Lys Arg 
    130 


<210>  12
<211>  617
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS3

<400>  12

Ser Gly Ala Leu Trp Asp Val Pro Ala Pro Lys Glu Val Lys Lys Gly 
1               5                   10                  15      


Glu Thr Thr Asp Gly Val Tyr Arg Val Met Thr Arg Arg Leu Leu Gly 
            20                  25                  30          


Ser Thr Gln Val Gly Val Gly Val Met Gln Glu Gly Val Phe His Thr 
        35                  40                  45              


Met Trp His Val Thr Lys Gly Ser Ala Leu Arg Ser Gly Glu Gly Arg 
    50                  55                  60                  


Leu Asp Pro Tyr Trp Gly Asp Val Lys Gln Asp Leu Val Ser Tyr Cys 
65                  70                  75                  80  


Gly Pro Trp Lys Leu Asp Ala Ala Trp Asp Gly His Ser Glu Val Gln 
                85                  90                  95      


Leu Leu Ala Val Pro Pro Gly Glu Arg Ala Arg Asn Ile Gln Thr Leu 
            100                 105                 110         


Pro Gly Ile Phe Lys Thr Lys Asp Gly Asp Ile Gly Ala Val Ala Leu 
        115                 120                 125             


Asp Tyr Pro Ala Gly Thr Ser Gly Ser Pro Ile Leu Asp Lys Cys Gly 
    130                 135                 140                 


Arg Val Ile Gly Leu Tyr Gly Asn Gly Val Val Ile Lys Asn Gly Ser 
145                 150                 155                 160 


Tyr Val Ser Ala Ile Thr Gln Gly Arg Arg Glu Glu Glu Thr Pro Val 
                165                 170                 175     


Glu Cys Phe Glu Pro Ser Met Leu Lys Lys Lys Gln Leu Thr Val Leu 
            180                 185                 190         


Asp Leu His Pro Gly Ala Gly Lys Thr Arg Arg Val Leu Pro Glu Ile 
        195                 200                 205             


Val Arg Glu Ala Ile Lys Thr Arg Leu Arg Thr Val Ile Leu Ala Pro 
    210                 215                 220                 


Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro 
225                 230                 235                 240 


Val Arg Tyr Met Thr Thr Ala Val Asn Val Thr His Ser Gly Thr Glu 
                245                 250                 255     


Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Ser Arg Leu Leu Gln 
            260                 265                 270         


Pro Ile Arg Val Pro Asn Tyr Asn Leu Tyr Ile Met Asp Glu Ala His 
        275                 280                 285             


Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg Gly Tyr Ile Ser Thr Arg 
    290                 295                 300                 


Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met Thr Ala Thr Pro Pro 
305                 310                 315                 320 


Gly Thr Arg Asp Ala Phe Pro Asp Ser Asn Ser Pro Ile Met Asp Thr 
                325                 330                 335     


Glu Val Glu Val Pro Glu Arg Ala Trp Ser Ser Gly Phe Asp Trp Val 
            340                 345                 350         


Thr Asp Tyr Ser Gly Lys Thr Val Trp Phe Val Pro Ser Val Arg Asn 
        355                 360                 365             


Gly Asn Glu Ile Ala Ala Cys Leu Thr Lys Ala Gly Lys Arg Val Ile 
    370                 375                 380                 


Gln Leu Ser Arg Lys Thr Phe Glu Thr Glu Phe Gln Lys Thr Lys His 
385                 390                 395                 400 


Gln Glu Trp Asp Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala 
                405                 410                 415     


Asn Phe Lys Ala Asp Arg Val Ile Asp Ser Arg Arg Cys Leu Lys Pro 
            420                 425                 430         


Val Ile Leu Asp Gly Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val 
        435                 440                 445             


Thr His Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro 
    450                 455                 460                 


Asn Lys Pro Gly Asp Glu Tyr Leu Tyr Gly Gly Gly Cys Ala Glu Thr 
465                 470                 475                 480 


Asp Glu Asp His Ala His Trp Leu Glu Ala Arg Met Leu Leu Asp Asn 
                485                 490                 495     


Ile Tyr Leu Gln Asp Gly Leu Ile Ala Ser Leu Tyr Arg Pro Glu Ala 
            500                 505                 510         


Asp Lys Val Ala Ala Ile Glu Gly Glu Phe Lys Leu Arg Thr Glu Gln 
        515                 520                 525             


Arg Lys Thr Phe Val Glu Leu Met Lys Arg Gly Asp Leu Pro Val Trp 
    530                 535                 540                 


Leu Ala Tyr Gln Val Ala Ser Ala Gly Ile Thr Tyr Thr Asp Arg Arg 
545                 550                 555                 560 


Trp Cys Phe Asp Gly Thr Thr Asn Asn Thr Ile Met Glu Asp Ser Val 
                565                 570                 575     


Pro Ala Glu Val Trp Thr Arg His Gly Glu Lys Arg Val Leu Lys Pro 
            580                 585                 590         


Arg Trp Met Asp Ala Arg Val Cys Ser Asp His Ala Ala Leu Lys Ser 
        595                 600                 605             


Phe Lys Glu Phe Ala Ala Gly Lys Arg 
    610                 615         


<210>  13
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS4A

<400>  13

Gly Ala Ala Phe Gly Val Met Glu Ala Leu Gly Thr Leu Pro Gly His 
1               5                   10                  15      


Met Thr Glu Arg Phe Gln Glu Ala Ile Asp Asn Leu Ala Val Leu Met 
            20                  25                  30          


Arg Ala Glu Thr Gly Ser Arg Pro Tyr Lys Ala Ala Ala Ala Gln Leu 
        35                  40                  45              


Pro Glu Thr Leu Glu Thr Ile Met Leu Leu Gly Leu Leu Gly Thr Val 
    50                  55                  60                  


Ser Leu Gly Ile Phe Phe Val Leu Met Arg Asn Lys Gly Ile Gly Lys 
65                  70                  75                  80  


Met Gly Phe Gly Met Val Thr Leu Gly Ala Ser Ala Trp Leu Met Trp 
                85                  90                  95      


Leu Ser Glu Ile Glu Pro Ala Arg Ile Ala Cys Val Leu Ile Val Val 
            100                 105                 110         


Phe Leu Leu Leu Val Val Leu Ile Pro Glu Pro Glu Lys Gln Arg Ser 
        115                 120                 125             


Pro Gln Asp Asn Gln Met Ala Ile Ile Ile Met Val Ala Val Gly Leu 
    130                 135                 140                 


Leu Gly Leu Ile Thr Ala 
145                 150 


<210>  14
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS4B

<400>  14

Asn Glu Leu Gly Trp Leu Glu Arg Thr Lys Ser Asp Leu Ser His Leu 
1               5                   10                  15      


Met Gly Arg Arg Glu Glu Gly Ala Thr Met Gly Phe Ser Met Asp Ile 
            20                  25                  30          


Asp Leu Arg Pro Ala Ser Ala Trp Ala Ile Tyr Ala Ala Leu Thr Thr 
        35                  40                  45              


Phe Ile Thr Pro Ala Val Gln His Ala Val Thr Thr Ser Tyr Asn Asn 
    50                  55                  60                  


Tyr Ser Leu Met Ala Met Ala Thr Gln Ala Gly Val Leu Phe Gly Met 
65                  70                  75                  80  


Gly Lys Gly Met Pro Phe Tyr Ala Trp Asp Phe Gly Val Pro Leu Leu 
                85                  90                  95      


Met Ile Gly Cys Tyr Ser Gln Leu Thr Pro Leu Thr Leu Ile Val Ala 
            100                 105                 110         


Ile Ile Leu Leu Val Ala His Tyr Met Tyr Leu Ile Pro Gly Leu Gln 
        115                 120                 125             


Ala Ala Ala Ala Arg Ala Ala Gln Lys Arg Thr Ala Ala Gly Ile Met 
    130                 135                 140                 


Lys Asn Pro Val Val Asp Gly Ile Val Val Thr Asp Ile Asp Thr Met 
145                 150                 155                 160 


Thr Ile Asp Pro Gln Val Glu Lys Lys Met Gly Gln Val Leu Leu Met 
                165                 170                 175     


Ala Val Ala Val Ser Ser Ala Ile Leu Ser Arg Thr Ala Trp Gly Trp 
            180                 185                 190         


Gly Glu Ala Gly Ala Leu Ile Thr Ala Ala Thr Ser Thr Leu Trp Glu 
        195                 200                 205             


Gly Ser Pro Asn Lys Tyr Trp Asn Ser Ser Thr Ala Thr Ser Leu Cys 
    210                 215                 220                 


Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Ser Leu Ile Tyr Thr 
225                 230                 235                 240 


Val Thr Arg Asn Ala Gly Leu Val Lys Arg Arg 
                245                 250     


<210>  15
<211>  903
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS5

<400>  15

Gly Gly Gly Thr Gly Glu Thr Leu Gly Glu Lys Trp Lys Ala Arg Leu 
1               5                   10                  15      


Asn Gln Met Ser Ala Leu Glu Phe Tyr Ser Tyr Lys Lys Ser Gly Ile 
            20                  25                  30          


Thr Glu Val Cys Arg Glu Glu Ala Arg Arg Ala Leu Lys Asp Gly Val 
        35                  40                  45              


Ala Thr Gly Gly His Ala Val Ser Arg Gly Ser Ala Lys Leu Arg Trp 
    50                  55                  60                  


Leu Val Glu Arg Gly Tyr Leu Gln Pro Tyr Gly Lys Val Ile Asp Leu 
65                  70                  75                  80  


Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Ala Ala Thr Ile Arg Lys 
                85                  90                  95      


Val Gln Glu Val Lys Gly Tyr Thr Lys Gly Gly Pro Gly His Glu Glu 
            100                 105                 110         


Pro Val Leu Val Gln Ser Tyr Gly Trp Asn Ile Val Arg Leu Lys Ser 
        115                 120                 125             


Gly Val Asp Val Phe His Met Ala Ala Glu Pro Cys Asp Thr Leu Leu 
    130                 135                 140                 


Cys Asp Ile Gly Glu Ser Ser Ser Ser Pro Glu Val Glu Glu Ala Arg 
145                 150                 155                 160 


Thr Leu Arg Val Leu Ser Met Val Gly Asp Trp Leu Glu Lys Arg Pro 
                165                 170                 175     


Gly Ala Phe Cys Ile Lys Val Leu Cys Pro Tyr Thr Ser Thr Met Met 
            180                 185                 190         


Glu Thr Leu Glu Arg Leu Gln Arg Arg Tyr Gly Gly Gly Leu Val Arg 
        195                 200                 205             


Val Pro Leu Ser Arg Asn Ser Thr His Glu Met Tyr Trp Val Ser Gly 
    210                 215                 220                 


Ala Lys Ser Asn Thr Ile Lys Ser Val Ser Thr Thr Ser Gln Leu Leu 
225                 230                 235                 240 


Leu Gly Arg Met Asp Gly Pro Arg Arg Pro Val Lys Tyr Glu Glu Asp 
                245                 250                 255     


Val Asn Leu Gly Ser Gly Thr Arg Ala Val Val Ser Cys Ala Glu Ala 
            260                 265                 270         


Pro Asn Met Lys Ile Ile Gly Asn Arg Ile Glu Arg Ile Arg Ser Glu 
        275                 280                 285             


His Ala Glu Thr Trp Phe Phe Asp Glu Asn His Pro Tyr Arg Thr Trp 
    290                 295                 300                 


Ala Tyr His Gly Ser Tyr Glu Ala Pro Thr Gln Gly Ser Ala Ser Ser 
305                 310                 315                 320 


Leu Ile Asn Gly Val Val Arg Leu Leu Ser Lys Pro Trp Asp Val Val 
                325                 330                 335     


Thr Gly Val Thr Gly Ile Ala Met Thr Asp Thr Thr Pro Tyr Gly Gln 
            340                 345                 350         


Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Val Pro Asp Pro Gln 
        355                 360                 365             


Glu Gly Thr Arg Gln Val Met Ser Met Val Ser Ser Trp Leu Trp Lys 
    370                 375                 380                 


Glu Leu Gly Lys His Lys Arg Pro Arg Val Cys Thr Lys Glu Glu Phe 
385                 390                 395                 400 


Ile Asn Lys Val Arg Ser Asn Ala Ala Leu Gly Ala Ile Phe Glu Glu 
                405                 410                 415     


Glu Lys Glu Trp Lys Thr Ala Val Glu Ala Val Asn Asp Pro Arg Phe 
            420                 425                 430         


Trp Ala Leu Val Asp Lys Glu Arg Glu His His Leu Arg Gly Glu Cys 
        435                 440                 445             


Gln Ser Cys Val Tyr Asn Met Met Gly Lys Arg Glu Lys Lys Gln Gly 
    450                 455                 460                 


Glu Phe Gly Lys Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu 
465                 470                 475                 480 


Gly Ala Arg Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp 
                485                 490                 495     


His Trp Met Gly Arg Glu Asn Ser Gly Gly Gly Val Glu Gly Leu Gly 
            500                 505                 510         


Leu Gln Arg Leu Gly Tyr Val Leu Glu Glu Met Ser Arg Ile Pro Gly 
        515                 520                 525             


Gly Arg Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Ser 
    530                 535                 540                 


Arg Phe Asp Leu Glu Asn Glu Ala Leu Ile Thr Asn Gln Met Glu Lys 
545                 550                 555                 560 


Gly His Arg Ala Leu Ala Leu Ala Ile Ile Lys Tyr Thr Tyr Gln Asn 
                565                 570                 575     


Lys Val Val Lys Val Leu Arg Pro Ala Glu Lys Gly Lys Thr Val Met 
            580                 585                 590         


Asp Ile Ile Ser Arg Gln Asp Gln Arg Gly Ser Gly Gln Val Val Thr 
        595                 600                 605             


Tyr Ala Leu Asn Thr Phe Thr Asn Leu Val Val Gln Leu Ile Arg Asn 
    610                 615                 620                 


Met Glu Ala Glu Glu Val Leu Glu Met Gln Asp Leu Trp Leu Leu Arg 
625                 630                 635                 640 


Arg Ser Glu Lys Val Thr Asn Trp Leu Gln Ser Asn Gly Trp Asp Arg 
                645                 650                 655     


Leu Lys Arg Met Ala Val Ser Gly Asp Asp Cys Val Val Lys Pro Ile 
            660                 665                 670         


Asp Asp Arg Phe Ala His Ala Leu Arg Phe Leu Asn Asp Met Gly Lys 
        675                 680                 685             


Val Arg Lys Asp Thr Gln Glu Trp Lys Pro Ser Thr Gly Trp Asp Asn 
    690                 695                 700                 


Trp Glu Glu Val Pro Phe Cys Ser His His Phe Asn Lys Leu His Leu 
705                 710                 715                 720 


Lys Asp Gly Arg Ser Ile Val Val Pro Cys Arg His Gln Asp Glu Leu 
                725                 730                 735     


Ile Gly Arg Ala Arg Val Ser Pro Gly Ala Gly Trp Ser Ile Arg Glu 
            740                 745                 750         


Thr Ala Cys Leu Ala Lys Ser Tyr Ala Gln Met Trp Gln Leu Leu Tyr 
        755                 760                 765             


Phe His Arg Arg Asp Leu Arg Leu Met Ala Asn Ala Ile Cys Ser Ser 
    770                 775                 780                 


Val Pro Val Asp Trp Val Pro Thr Gly Arg Thr Thr Trp Ser Ile His 
785                 790                 795                 800 


Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Val Val Trp Asn 
                805                 810                 815     


Arg Val Trp Ile Glu Glu Asn Asp His Met Glu Asp Lys Thr Pro Val 
            820                 825                 830         


Thr Lys Trp Thr Asp Ile Pro Tyr Leu Gly Lys Arg Glu Asp Leu Trp 
        835                 840                 845             


Cys Gly Ser Leu Ile Gly His Arg Pro Arg Thr Thr Trp Ala Glu Asn 
    850                 855                 860                 


Ile Lys Asn Thr Val Asn Met Val Arg Arg Ile Ile Gly Asp Glu Glu 
865                 870                 875                 880 


Lys Tyr Met Asp Tyr Leu Ser Thr Gln Val Arg Tyr Leu Gly Glu Glu 
                885                 890                 895     


Gly Ser Thr Pro Gly Val Leu 
            900             


<210>  16
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804)

<400>  16

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  17
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723)

<400>  17

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  18
<211>  3423
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein

<400>  18

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
    130                 135                 140                 


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Thr Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
        435                 440                 445             


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
    450                 455                 460                 


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
465                 470                 475                 480 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
                485                 490                 495     


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
            500                 505                 510         


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
        515                 520                 525             


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
    530                 535                 540                 


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
545                 550                 555                 560 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
            580                 585                 590         


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
        595                 600                 605             


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
    610                 615                 620                 


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
625                 630                 635                 640 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
                645                 650                 655     


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
        675                 680                 685             


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
    690                 695                 700                 


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
                725                 730                 735     


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
        755                 760                 765             


Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
    770                 775                 780                 


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
785                 790                 795                 800 


Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr 
                805                 810                 815     


Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser 
            820                 825                 830         


Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile 
        835                 840                 845             


Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser 
    850                 855                 860                 


Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu 
865                 870                 875                 880 


Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln 
                885                 890                 895     


Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp 
            900                 905                 910         


Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val 
        915                 920                 925             


Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp 
    930                 935                 940                 


Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser 
945                 950                 955                 960 


Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala 
                965                 970                 975     


Val Ile Gly Thr Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu 
            980                 985                 990         


Gly Tyr Trp Ile Glu Ser Glu Lys  Asn Asp Thr Trp Arg  Leu Lys Arg 
        995                 1000                 1005             


Ala His  Leu Ile Glu Met Lys  Thr Cys Glu Trp Pro  Lys Ser His 
    1010                 1015                 1020             


Thr Leu  Trp Thr Asp Gly Ile  Glu Glu Ser Asp Leu  Ile Ile Pro 
    1025                 1030                 1035             


Lys Ser  Leu Ala Gly Pro Leu  Ser His His Asn Thr  Arg Glu Gly 
    1040                 1045                 1050             


Tyr Arg  Thr Gln Met Lys Gly  Pro Trp His Ser Glu  Glu Leu Glu 
    1055                 1060                 1065             


Ile Arg  Phe Glu Glu Cys Pro  Gly Thr Lys Val His  Val Glu Glu 
    1070                 1075                 1080             


Thr Cys  Gly Thr Arg Gly Pro  Ser Leu Arg Ser Thr  Thr Ala Ser 
    1085                 1090                 1095             


Gly Arg  Val Ile Glu Glu Trp  Cys Cys Arg Glu Cys  Thr Met Pro 
    1100                 1105                 1110             


Pro Leu  Ser Phe Arg Ala Lys  Asp Gly Cys Trp Tyr  Gly Met Glu 
    1115                 1120                 1125             


Ile Arg  Pro Arg Lys Glu Pro  Glu Ser Asn Leu Val  Arg Ser Met 
    1130                 1135                 1140             


Val Thr  Ala Gly Ser Thr Asp  His Met Asp His Phe  Ser Leu Gly 
    1145                 1150                 1155             


Val Leu  Val Ile Leu Leu Met  Val Gln Glu Gly Leu  Lys Lys Arg 
    1160                 1165                 1170             


Met Thr  Thr Lys Ile Ile Ile  Ser Thr Ser Met Ala  Val Leu Val 
    1175                 1180                 1185             


Ala Met  Ile Leu Gly Gly Phe  Ser Met Ser Asp Leu  Ala Lys Leu 
    1190                 1195                 1200             


Ala Ile  Leu Met Gly Ala Thr  Phe Ala Glu Met Asn  Thr Gly Gly 
    1205                 1210                 1215             


Asp Val  Ala His Leu Ala Leu  Ile Ala Ala Phe Lys  Val Arg Pro 
    1220                 1225                 1230             


Ala Leu  Leu Val Ser Phe Ile  Phe Arg Ala Asn Trp  Thr Pro Arg 
    1235                 1240                 1245             


Glu Ser  Met Leu Leu Ala Leu  Ala Ser Cys Leu Leu  Gln Thr Ala 
    1250                 1255                 1260             


Ile Ser  Ala Leu Glu Gly Asp  Leu Met Val Leu Ile  Asn Gly Phe 
    1265                 1270                 1275             


Ala Leu  Ala Trp Leu Ala Ile  Arg Ala Met Val Val  Pro Arg Thr 
    1280                 1285                 1290             


Asp Asn  Ile Thr Leu Ala Ile  Leu Ala Ala Leu Thr  Pro Leu Ala 
    1295                 1300                 1305             


Arg Gly  Thr Leu Leu Val Ala  Trp Arg Ala Gly Leu  Ala Thr Cys 
    1310                 1315                 1320             


Gly Gly  Phe Met Leu Leu Ser  Leu Lys Gly Lys Gly  Ser Val Lys 
    1325                 1330                 1335             


Lys Asn  Leu Pro Phe Val Met  Ala Leu Gly Leu Thr  Ala Val Arg 
    1340                 1345                 1350             


Leu Val  Asp Pro Ile Asn Val  Val Gly Leu Leu Leu  Leu Thr Arg 
    1355                 1360                 1365             


Ser Gly  Lys Arg Ser Trp Pro  Pro Ser Glu Val Leu  Thr Ala Val 
    1370                 1375                 1380             


Gly Leu  Ile Cys Ala Leu Ala  Gly Gly Phe Ala Lys  Ala Asp Ile 
    1385                 1390                 1395             


Glu Met  Ala Gly Pro Met Ala  Ala Val Gly Leu Leu  Ile Val Ser 
    1400                 1405                 1410             


Tyr Val  Val Ser Gly Lys Ser  Val Asp Met Tyr Ile  Glu Arg Ala 
    1415                 1420                 1425             


Gly Asp  Ile Thr Trp Glu Lys  Asp Ala Glu Val Thr  Gly Asn Ser 
    1430                 1435                 1440             


Pro Arg  Leu Asp Val Ala Leu  Asp Glu Ser Gly Asp  Phe Ser Leu 
    1445                 1450                 1455             


Val Glu  Asp Asp Gly Pro Pro  Met Arg Glu Ile Ile  Leu Lys Val 
    1460                 1465                 1470             


Val Leu  Met Thr Ile Cys Gly  Met Asn Pro Ile Ala  Ile Pro Phe 
    1475                 1480                 1485             


Ala Ala  Gly Ala Trp Tyr Val  Tyr Val Lys Thr Gly  Lys Arg Ser 
    1490                 1495                 1500             


Gly Ala  Leu Trp Asp Val Pro  Ala Pro Lys Glu Val  Lys Lys Gly 
    1505                 1510                 1515             


Glu Thr  Thr Asp Gly Val Tyr  Arg Val Met Thr Arg  Arg Leu Leu 
    1520                 1525                 1530             


Gly Ser  Thr Gln Val Gly Val  Gly Val Met Gln Glu  Gly Val Phe 
    1535                 1540                 1545             


His Thr  Met Trp His Val Thr  Lys Gly Ser Ala Leu  Arg Ser Gly 
    1550                 1555                 1560             


Glu Gly  Arg Leu Asp Pro Tyr  Trp Gly Asp Val Lys  Gln Asp Leu 
    1565                 1570                 1575             


Val Ser  Tyr Cys Gly Pro Trp  Lys Leu Asp Ala Ala  Trp Asp Gly 
    1580                 1585                 1590             


His Ser  Glu Val Gln Leu Leu  Ala Val Pro Pro Gly  Glu Arg Ala 
    1595                 1600                 1605             


Arg Asn  Ile Gln Thr Leu Pro  Gly Ile Phe Lys Thr  Lys Asp Gly 
    1610                 1615                 1620             


Asp Ile  Gly Ala Val Ala Leu  Asp Tyr Pro Ala Gly  Thr Ser Gly 
    1625                 1630                 1635             


Ser Pro  Ile Leu Asp Lys Cys  Gly Arg Val Ile Gly  Leu Tyr Gly 
    1640                 1645                 1650             


Asn Gly  Val Val Ile Lys Asn  Gly Ser Tyr Val Ser  Ala Ile Thr 
    1655                 1660                 1665             


Gln Gly  Arg Arg Glu Glu Glu  Thr Pro Val Glu Cys  Phe Glu Pro 
    1670                 1675                 1680             


Ser Met  Leu Lys Lys Lys Gln  Leu Thr Val Leu Asp  Leu His Pro 
    1685                 1690                 1695             


Gly Ala  Gly Lys Thr Arg Arg  Val Leu Pro Glu Ile  Val Arg Glu 
    1700                 1705                 1710             


Ala Ile  Lys Thr Arg Leu Arg  Thr Val Ile Leu Ala  Pro Thr Arg 
    1715                 1720                 1725             


Val Val  Ala Ala Glu Met Glu  Glu Ala Leu Arg Gly  Leu Pro Val 
    1730                 1735                 1740             


Arg Tyr  Met Thr Thr Ala Val  Asn Val Thr His Ser  Gly Thr Glu 
    1745                 1750                 1755             


Ile Val  Asp Leu Met Cys His  Ala Thr Phe Thr Ser  Arg Leu Leu 
    1760                 1765                 1770             


Gln Pro  Ile Arg Val Pro Asn  Tyr Asn Leu Tyr Ile  Met Asp Glu 
    1775                 1780                 1785             


Ala His  Phe Thr Asp Pro Ser  Ser Ile Ala Ala Arg  Gly Tyr Ile 
    1790                 1795                 1800             


Ser Thr  Arg Val Glu Met Gly  Glu Ala Ala Ala Ile  Phe Met Thr 
    1805                 1810                 1815             


Ala Thr  Pro Pro Gly Thr Arg  Asp Ala Phe Pro Asp  Ser Asn Ser 
    1820                 1825                 1830             


Pro Ile  Met Asp Thr Glu Val  Glu Val Pro Glu Arg  Ala Trp Ser 
    1835                 1840                 1845             


Ser Gly  Phe Asp Trp Val Thr  Asp His Ser Gly Lys  Thr Val Trp 
    1850                 1855                 1860             


Phe Val  Pro Ser Val Arg Asn  Gly Asn Glu Ile Ala  Ala Cys Leu 
    1865                 1870                 1875             


Thr Lys  Ala Gly Lys Arg Val  Ile Gln Leu Ser Arg  Lys Thr Phe 
    1880                 1885                 1890             


Glu Thr  Glu Phe Gln Lys Thr  Lys His Gln Glu Trp  Asp Phe Val 
    1895                 1900                 1905             


Val Thr  Thr Asp Ile Ser Glu  Met Gly Ala Asn Phe  Lys Ala Asp 
    1910                 1915                 1920             


Arg Val  Ile Asp Ser Arg Arg  Cys Leu Lys Pro Val  Ile Leu Asp 
    1925                 1930                 1935             


Gly Glu  Arg Val Ile Leu Ala  Gly Pro Met Pro Val  Thr His Ala 
    1940                 1945                 1950             


Ser Ala  Ala Gln Arg Arg Gly  Arg Ile Gly Arg Asn  Pro Asn Lys 
    1955                 1960                 1965             


Pro Gly  Asp Glu Tyr Leu Tyr  Gly Gly Gly Cys Ala  Glu Thr Asp 
    1970                 1975                 1980             


Glu Asp  His Ala His Trp Leu  Glu Ala Arg Met Leu  Leu Asp Asn 
    1985                 1990                 1995             


Ile Tyr  Leu Gln Asp Gly Leu  Ile Ala Ser Leu Tyr  Arg Pro Glu 
    2000                 2005                 2010             


Ala Asp  Lys Val Ala Ala Ile  Glu Gly Glu Phe Lys  Leu Arg Thr 
    2015                 2020                 2025             


Glu Gln  Arg Lys Thr Phe Val  Glu Leu Met Lys Arg  Gly Asp Leu 
    2030                 2035                 2040             


Pro Val  Trp Leu Ala Tyr Gln  Val Ala Ser Ala Gly  Ile Thr Tyr 
    2045                 2050                 2055             


Thr Asp  Arg Arg Trp Cys Phe  Asp Gly Thr Thr Asn  Asn Thr Ile 
    2060                 2065                 2070             


Met Glu  Asp Ser Val Pro Ala  Glu Val Trp Thr Arg  His Gly Glu 
    2075                 2080                 2085             


Lys Arg  Val Leu Lys Pro Arg  Trp Met Asp Ala Arg  Val Cys Ser 
    2090                 2095                 2100             


Asp His  Ala Ala Leu Lys Ser  Phe Lys Glu Phe Ala  Ala Gly Lys 
    2105                 2110                 2115             


Arg Gly  Ala Ala Phe Gly Val  Met Glu Ala Leu Gly  Thr Leu Pro 
    2120                 2125                 2130             


Gly His  Met Thr Glu Arg Phe  Gln Glu Ala Ile Asp  Asn Leu Ala 
    2135                 2140                 2145             


Val Leu  Met Arg Ala Glu Thr  Gly Ser Arg Pro Tyr  Lys Ala Ala 
    2150                 2155                 2160             


Ala Ala  Gln Leu Pro Glu Thr  Leu Glu Thr Ile Met  Leu Leu Gly 
    2165                 2170                 2175             


Leu Leu  Gly Thr Val Ser Leu  Gly Ile Phe Phe Val  Leu Met Arg 
    2180                 2185                 2190             


Asn Lys  Gly Ile Gly Lys Met  Gly Phe Gly Met Val  Thr Leu Gly 
    2195                 2200                 2205             


Ala Ser  Ala Trp Leu Met Trp  Leu Ser Glu Ile Glu  Pro Ala Arg 
    2210                 2215                 2220             


Ile Ala  Cys Val Leu Ile Val  Val Phe Leu Leu Leu  Val Val Leu 
    2225                 2230                 2235             


Ile Pro  Glu Pro Glu Lys Gln  Arg Ser Pro Gln Asp  Asn Gln Met 
    2240                 2245                 2250             


Ala Ile  Ile Ile Met Val Ala  Val Gly Leu Leu Gly  Leu Ile Thr 
    2255                 2260                 2265             


Ala Asn  Glu Leu Gly Trp Leu  Glu Arg Thr Lys Ser  Asp Leu Ser 
    2270                 2275                 2280             


His Leu  Met Gly Arg Arg Glu  Glu Gly Ala Thr Ile  Gly Phe Ser 
    2285                 2290                 2295             


Met Asp  Ile Asp Leu Arg Pro  Ala Ser Ala Trp Ala  Ile Tyr Ala 
    2300                 2305                 2310             


Ala Leu  Thr Thr Phe Ile Thr  Pro Ala Val Gln His  Ala Val Thr 
    2315                 2320                 2325             


Thr Ser  Tyr Asn Asn Tyr Ser  Leu Met Ala Met Ala  Thr Gln Ala 
    2330                 2335                 2340             


Gly Val  Leu Phe Gly Met Gly  Lys Gly Met Pro Phe  Tyr Ala Trp 
    2345                 2350                 2355             


Asp Phe  Gly Val Pro Leu Leu  Met Ile Gly Cys Tyr  Ser Gln Leu 
    2360                 2365                 2370             


Thr Pro  Leu Thr Leu Ile Val  Ala Ile Ile Leu Leu  Val Ala His 
    2375                 2380                 2385             


Tyr Met  Tyr Leu Ile Pro Gly  Leu Gln Ala Ala Ala  Ala Arg Ala 
    2390                 2395                 2400             


Ala Gln  Lys Arg Thr Ala Ala  Gly Ile Met Lys Asn  Pro Val Val 
    2405                 2410                 2415             


Asp Gly  Ile Val Val Thr Asp  Ile Asp Thr Met Thr  Ile Asp Pro 
    2420                 2425                 2430             


Gln Val  Glu Lys Lys Met Gly  Gln Val Leu Leu Ile  Ala Val Ala 
    2435                 2440                 2445             


Val Ser  Ser Ala Ile Leu Ser  Arg Thr Ala Trp Gly  Trp Gly Glu 
    2450                 2455                 2460             


Ala Gly  Ala Leu Ile Thr Ala  Ala Thr Ser Thr Leu  Trp Glu Gly 
    2465                 2470                 2475             


Ser Pro  Asn Lys Tyr Trp Asn  Ser Ser Thr Ala Thr  Ser Leu Cys 
    2480                 2485                 2490             


Asn Ile  Phe Arg Gly Ser Tyr  Leu Ala Gly Ala Ser  Leu Ile Tyr 
    2495                 2500                 2505             


Thr Val  Thr Arg Asn Ala Gly  Leu Val Lys Arg Arg  Gly Gly Gly 
    2510                 2515                 2520             


Thr Gly  Glu Thr Leu Gly Glu  Lys Trp Lys Ala Arg  Leu Asn Gln 
    2525                 2530                 2535             


Met Ser  Ala Leu Glu Phe Tyr  Ser Tyr Lys Lys Ser  Gly Ile Thr 
    2540                 2545                 2550             


Glu Val  Cys Arg Glu Glu Ala  Arg Arg Ala Leu Lys  Asp Gly Val 
    2555                 2560                 2565             


Ala Thr  Gly Gly His Ala Val  Ser Arg Gly Ser Ala  Lys Leu Arg 
    2570                 2575                 2580             


Trp Leu  Val Glu Arg Gly Tyr  Leu Gln Pro Tyr Gly  Lys Val Ile 
    2585                 2590                 2595             


Asp Leu  Gly Cys Gly Arg Gly  Gly Trp Ser Tyr Tyr  Ala Ala Thr 
    2600                 2605                 2610             


Ile Arg  Lys Val Gln Glu Val  Lys Gly Tyr Thr Lys  Gly Gly Pro 
    2615                 2620                 2625             


Gly His  Glu Glu Pro Val Leu  Val Gln Ser Tyr Gly  Trp Asn Ile 
    2630                 2635                 2640             


Val Arg  Leu Lys Ser Gly Val  Asp Val Phe His Met  Ala Ala Glu 
    2645                 2650                 2655             


Pro Cys  Asp Thr Leu Leu Cys  Asp Ile Gly Glu Ser  Ser Ser Ser 
    2660                 2665                 2670             


Pro Glu  Val Glu Glu Ala Arg  Thr Leu Arg Val Leu  Ser Met Val 
    2675                 2680                 2685             


Gly Asp  Trp Leu Glu Lys Arg  Pro Gly Ala Phe Cys  Ile Lys Val 
    2690                 2695                 2700             


Leu Cys  Pro Tyr Thr Ser Thr  Met Met Glu Thr Leu  Glu Arg Leu 
    2705                 2710                 2715             


Gln Arg  Arg Tyr Gly Gly Gly  Leu Val Arg Val Pro  Leu Ser Arg 
    2720                 2725                 2730             


Asn Ser  Thr His Glu Met Tyr  Trp Val Ser Gly Ala  Lys Ser Asn 
    2735                 2740                 2745             


Thr Ile  Lys Ser Val Ser Thr  Thr Ser Gln Leu Leu  Leu Gly Arg 
    2750                 2755                 2760             


Met Asp  Gly Pro Arg Arg Pro  Val Lys Tyr Glu Glu  Asp Val Asn 
    2765                 2770                 2775             


Leu Gly  Ser Gly Thr Arg Ala  Val Val Ser Cys Ala  Glu Ala Pro 
    2780                 2785                 2790             


Asn Met  Lys Ile Ile Gly Asn  Arg Ile Glu Arg Ile  Arg Ser Glu 
    2795                 2800                 2805             


His Ala  Glu Thr Trp Phe Phe  Asp Glu Asn His Pro  Tyr Arg Thr 
    2810                 2815                 2820             


Trp Ala  Tyr His Gly Ser Tyr  Glu Ala Pro Thr Gln  Gly Ser Ala 
    2825                 2830                 2835             


Ser Ser  Leu Ile Asn Gly Val  Val Arg Leu Leu Ser  Lys Pro Trp 
    2840                 2845                 2850             


Asp Val  Val Thr Gly Val Thr  Gly Ile Ala Met Thr  Asp Thr Thr 
    2855                 2860                 2865             


Pro Tyr  Gly Gln Gln Arg Val  Phe Lys Glu Lys Val  Asp Thr Arg 
    2870                 2875                 2880             


Val Pro  Asp Pro Gln Glu Gly  Thr Arg Gln Val Met  Ser Met Val 
    2885                 2890                 2895             


Ser Ser  Trp Leu Trp Lys Glu  Leu Gly Lys His Lys  Arg Pro Arg 
    2900                 2905                 2910             


Val Cys  Thr Lys Glu Glu Phe  Ile Asn Lys Val Arg  Ser Asn Ala 
    2915                 2920                 2925             


Ala Leu  Gly Ala Ile Phe Glu  Glu Glu Lys Glu Trp  Lys Thr Ala 
    2930                 2935                 2940             


Val Glu  Ala Val Asn Asp Pro  Arg Phe Trp Ala Leu  Val Asp Lys 
    2945                 2950                 2955             


Glu Arg  Glu His His Leu Arg  Gly Glu Cys Gln Ser  Cys Val Tyr 
    2960                 2965                 2970             


Asn Met  Met Gly Lys Arg Glu  Lys Lys Gln Gly Glu  Phe Gly Lys 
    2975                 2980                 2985             


Ala Lys  Gly Ser Arg Ala Ile  Trp Tyr Met Trp Leu  Gly Ala Arg 
    2990                 2995                 3000             


Phe Leu  Glu Phe Glu Ala Leu  Gly Phe Leu Asn Glu  Asp His Trp 
    3005                 3010                 3015             


Met Gly  Arg Glu Asn Ser Gly  Gly Gly Val Glu Gly  Leu Gly Leu 
    3020                 3025                 3030             


Gln Arg  Leu Gly Tyr Val Leu  Glu Glu Met Ser Arg  Ile Pro Gly 
    3035                 3040                 3045             


Gly Arg  Met Tyr Ala Asp Asp  Thr Ala Gly Trp Asp  Thr Arg Ile 
    3050                 3055                 3060             


Ser Arg  Phe Asp Leu Glu Asn  Glu Ala Leu Ile Thr  Asn Gln Met 
    3065                 3070                 3075             


Glu Lys  Gly His Arg Ala Leu  Ala Leu Ala Ile Ile  Lys Tyr Thr 
    3080                 3085                 3090             


Tyr Gln  Asn Lys Val Val Lys  Val Leu Arg Pro Ala  Glu Lys Gly 
    3095                 3100                 3105             


Lys Thr  Val Met Asp Ile Ile  Ser Arg Gln Asp Gln  Arg Gly Ser 
    3110                 3115                 3120             


Gly Gln  Val Val Thr Tyr Ala  Leu Asn Thr Phe Thr  Asn Leu Val 
    3125                 3130                 3135             


Val Gln  Leu Ile Arg Asn Met  Glu Ala Glu Glu Val  Leu Glu Met 
    3140                 3145                 3150             


Gln Asp  Leu Trp Leu Leu Arg  Arg Ser Glu Lys Val  Thr Asn Trp 
    3155                 3160                 3165             


Leu Gln  Ser Asn Gly Trp Asp  Arg Leu Lys Arg Met  Ala Val Ser 
    3170                 3175                 3180             


Gly Asp  Asp Cys Val Val Lys  Pro Ile Asp Asp Arg  Phe Ala His 
    3185                 3190                 3195             


Ala Leu  Arg Phe Leu Asn Asp  Met Gly Lys Val Arg  Lys Asp Thr 
    3200                 3205                 3210             


Gln Glu  Trp Lys Pro Ser Thr  Gly Trp Asp Asn Trp  Glu Glu Val 
    3215                 3220                 3225             


Pro Phe  Cys Ser His His Phe  Asn Lys Leu His Leu  Lys Asp Gly 
    3230                 3235                 3240             


Arg Ser  Ile Val Val Pro Cys  Arg His Gln Asp Glu  Leu Ile Gly 
    3245                 3250                 3255             


Arg Ala  Arg Val Ser Pro Gly  Ala Gly Trp Ser Ile  Arg Glu Thr 
    3260                 3265                 3270             


Ala Cys  Leu Ala Lys Ser Tyr  Ala Gln Met Trp Gln  Leu Leu Tyr 
    3275                 3280                 3285             


Phe His  Arg Arg Asp Leu Arg  Leu Met Ala Asn Ala  Ile Cys Ser 
    3290                 3295                 3300             


Ser Val  Pro Val Asp Trp Val  Pro Thr Gly Arg Thr  Thr Trp Ser 
    3305                 3310                 3315             


Ile His  Gly Lys Gly Glu Trp  Met Thr Thr Glu Asp  Met Leu Val 
    3320                 3325                 3330             


Val Trp  Asn Arg Val Trp Ile  Glu Glu Asn Asp His  Met Glu Asp 
    3335                 3340                 3345             


Lys Thr  Pro Val Thr Lys Trp  Thr Asp Ile Pro Tyr  Leu Gly Lys 
    3350                 3355                 3360             


Arg Glu  Asp Leu Trp Cys Gly  Ser Leu Ile Gly His  Arg Pro Arg 
    3365                 3370                 3375             


Thr Thr  Trp Ala Glu Asn Ile  Lys Asn Thr Val Asn  Met Val Arg 
    3380                 3385                 3390             


Arg Ile  Ile Gly Asp Glu Glu  Lys Tyr Met Asp Tyr  Leu Ser Thr 
    3395                 3400                 3405             


Gln Val  Arg Tyr Leu Gly Glu  Glu Gly Ser Thr Pro  Gly Val Leu 
    3410                 3415                 3420             


<210>  19
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C

<400>  19

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg 
            100                 


<210>  20
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-ER anchor

<400>  20

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
        115                 120         


<210>  21
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) pr

<400>  21

Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
            20                  25                  30          


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
                85                  90              


<210>  22
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) M

<400>  22

Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
1               5                   10                  15      


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
            20                  25                  30          


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
        35                  40                  45              


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
    50                  55                  60                  


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
65                  70                  75  


<210>  23
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prM

<400>  23

Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
            20                  25                  30          


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
                85                  90                  95      


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
            100                 105                 110         


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
        115                 120                 125             


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
    130                 135                 140                 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
145                 150                 155                 160 


Leu Leu Ile Ala Pro Ala Tyr Ser 
                165             


<210>  24
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) E

<400>  24

Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
1               5                   10                  15      


Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
            20                  25                  30          


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
        35                  40                  45              


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
    50                  55                  60                  


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
65                  70                  75                  80  


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
                85                  90                  95      


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
            100                 105                 110         


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
        115                 120                 125             


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
    130                 135                 140                 


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
145                 150                 155                 160 


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
                165                 170                 175     


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
            180                 185                 190         


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
        195                 200                 205             


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
    210                 215                 220                 


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
225                 230                 235                 240 


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
                245                 250                 255     


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
            260                 265                 270         


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
        275                 280                 285             


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
    290                 295                 300                 


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
305                 310                 315                 320 


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
                325                 330                 335     


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
            340                 345                 350         


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
        355                 360                 365             


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
    370                 375                 380                 


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
385                 390                 395                 400 


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
                405                 410                 415     


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
            420                 425                 430         


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
        435                 440                 445             


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
    450                 455                 460                 


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys 
465                 470                 475                 480 


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
                485                 490                 495     


Phe Leu Ser Thr Ala Val Ser Ala 
            500                 


<210>  25
<211>  672
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME

<400>  25

Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
            20                  25                  30          


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
                85                  90                  95      


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
            100                 105                 110         


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
        115                 120                 125             


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
    130                 135                 140                 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
145                 150                 155                 160 


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
                165                 170                 175     


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
            180                 185                 190         


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
        195                 200                 205             


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
    210                 215                 220                 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
225                 230                 235                 240 


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
                245                 250                 255     


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
            260                 265                 270         


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
        275                 280                 285             


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
    290                 295                 300                 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
305                 310                 315                 320 


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
                325                 330                 335     


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
            340                 345                 350         


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
        355                 360                 365             


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
    370                 375                 380                 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
385                 390                 395                 400 


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
                405                 410                 415     


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
            420                 425                 430         


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
        435                 440                 445             


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
    450                 455                 460                 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
465                 470                 475                 480 


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
                485                 490                 495     


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
            500                 505                 510         


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
        515                 520                 525             


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
    530                 535                 540                 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
545                 550                 555                 560 


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
                565                 570                 575     


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
            580                 585                 590         


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
        595                 600                 605             


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
    610                 615                 620                 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
625                 630                 635                 640 


Met Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys 
                645                 650                 655     


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
            660                 665                 670         


<210>  26
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS1

<400>  26

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu 
    50                  55                  60                  


Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu 
65                  70                  75                  80  


Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro 
                85                  90                  95      


Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro 
            100                 105                 110         


His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys 
        115                 120                 125             


Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro 
    130                 135                 140                 


Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe 
145                 150                 155                 160 


Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser 
                165                 170                 175     


Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Lys Glu 
            180                 185                 190         


Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp 
        195                 200                 205             


Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr 
                245                 250                 255     


Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu 
            260                 265                 270         


Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu 
        275                 280                 285             


Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 
    290                 295                 300                 


Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr Ala 
            340                 345                 350         


<210>  27
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS2A

<400>  27

Gly Ser Thr Asp His Met Asp His Phe Ser Leu Gly Val Leu Val Ile 
1               5                   10                  15      


Leu Leu Met Val Gln Glu Gly Leu Lys Lys Arg Met Thr Thr Lys Ile 
            20                  25                  30          


Ile Ile Ser Thr Ser Met Ala Val Leu Val Ala Met Ile Leu Gly Gly 
        35                  40                  45              


Phe Ser Met Ser Asp Leu Ala Lys Leu Ala Ile Leu Met Gly Ala Thr 
    50                  55                  60                  


Phe Ala Glu Met Asn Thr Gly Gly Asp Val Ala His Leu Ala Leu Ile 
65                  70                  75                  80  


Ala Ala Phe Lys Val Arg Pro Ala Leu Leu Val Ser Phe Ile Phe Arg 
                85                  90                  95      


Ala Asn Trp Thr Pro Arg Glu Ser Met Leu Leu Ala Leu Ala Ser Cys 
            100                 105                 110         


Leu Leu Gln Thr Ala Ile Ser Ala Leu Glu Gly Asp Leu Met Val Leu 
        115                 120                 125             


Ile Asn Gly Phe Ala Leu Ala Trp Leu Ala Ile Arg Ala Met Val Val 
    130                 135                 140                 


Pro Arg Thr Asp Asn Ile Thr Leu Ala Ile Leu Ala Ala Leu Thr Pro 
145                 150                 155                 160 


Leu Ala Arg Gly Thr Leu Leu Val Ala Trp Arg Ala Gly Leu Ala Thr 
                165                 170                 175     


Cys Gly Gly Phe Met Leu Leu Ser Leu Lys Gly Lys Gly Ser Val Lys 
            180                 185                 190         


Lys Asn Leu Pro Phe Val Met Ala Leu Gly Leu Thr Ala Val Arg Leu 
        195                 200                 205             


Val Asp Pro Ile Asn Val Val Gly Leu Leu Leu Leu Thr Arg Ser Gly 
    210                 215                 220                 


Lys Arg 
225     


<210>  28
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS2B

<400>  28

Ser Trp Pro Pro Ser Glu Val Leu Thr Ala Val Gly Leu Ile Cys Ala 
1               5                   10                  15      


Leu Ala Gly Gly Phe Ala Lys Ala Asp Ile Glu Met Ala Gly Pro Met 
            20                  25                  30          


Ala Ala Val Gly Leu Leu Ile Val Ser Tyr Val Val Ser Gly Lys Ser 
        35                  40                  45              


Val Asp Met Tyr Ile Glu Arg Ala Gly Asp Ile Thr Trp Glu Lys Asp 
    50                  55                  60                  


Ala Glu Val Thr Gly Asn Ser Pro Arg Leu Asp Val Ala Leu Asp Glu 
65                  70                  75                  80  


Ser Gly Asp Phe Ser Leu Val Glu Asp Asp Gly Pro Pro Met Arg Glu 
                85                  90                  95      


Ile Ile Leu Lys Val Val Leu Met Thr Ile Cys Gly Met Asn Pro Ile 
            100                 105                 110         


Ala Ile Pro Phe Ala Ala Gly Ala Trp Tyr Val Tyr Val Lys Thr Gly 
        115                 120                 125             


Lys Arg 
    130 


<210>  29
<211>  617
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS3

<400>  29

Ser Gly Ala Leu Trp Asp Val Pro Ala Pro Lys Glu Val Lys Lys Gly 
1               5                   10                  15      


Glu Thr Thr Asp Gly Val Tyr Arg Val Met Thr Arg Arg Leu Leu Gly 
            20                  25                  30          


Ser Thr Gln Val Gly Val Gly Val Met Gln Glu Gly Val Phe His Thr 
        35                  40                  45              


Met Trp His Val Thr Lys Gly Ser Ala Leu Arg Ser Gly Glu Gly Arg 
    50                  55                  60                  


Leu Asp Pro Tyr Trp Gly Asp Val Lys Gln Asp Leu Val Ser Tyr Cys 
65                  70                  75                  80  


Gly Pro Trp Lys Leu Asp Ala Ala Trp Asp Gly His Ser Glu Val Gln 
                85                  90                  95      


Leu Leu Ala Val Pro Pro Gly Glu Arg Ala Arg Asn Ile Gln Thr Leu 
            100                 105                 110         


Pro Gly Ile Phe Lys Thr Lys Asp Gly Asp Ile Gly Ala Val Ala Leu 
        115                 120                 125             


Asp Tyr Pro Ala Gly Thr Ser Gly Ser Pro Ile Leu Asp Lys Cys Gly 
    130                 135                 140                 


Arg Val Ile Gly Leu Tyr Gly Asn Gly Val Val Ile Lys Asn Gly Ser 
145                 150                 155                 160 


Tyr Val Ser Ala Ile Thr Gln Gly Arg Arg Glu Glu Glu Thr Pro Val 
                165                 170                 175     


Glu Cys Phe Glu Pro Ser Met Leu Lys Lys Lys Gln Leu Thr Val Leu 
            180                 185                 190         


Asp Leu His Pro Gly Ala Gly Lys Thr Arg Arg Val Leu Pro Glu Ile 
        195                 200                 205             


Val Arg Glu Ala Ile Lys Thr Arg Leu Arg Thr Val Ile Leu Ala Pro 
    210                 215                 220                 


Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro 
225                 230                 235                 240 


Val Arg Tyr Met Thr Thr Ala Val Asn Val Thr His Ser Gly Thr Glu 
                245                 250                 255     


Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Ser Arg Leu Leu Gln 
            260                 265                 270         


Pro Ile Arg Val Pro Asn Tyr Asn Leu Tyr Ile Met Asp Glu Ala His 
        275                 280                 285             


Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg Gly Tyr Ile Ser Thr Arg 
    290                 295                 300                 


Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met Thr Ala Thr Pro Pro 
305                 310                 315                 320 


Gly Thr Arg Asp Ala Phe Pro Asp Ser Asn Ser Pro Ile Met Asp Thr 
                325                 330                 335     


Glu Val Glu Val Pro Glu Arg Ala Trp Ser Ser Gly Phe Asp Trp Val 
            340                 345                 350         


Thr Asp His Ser Gly Lys Thr Val Trp Phe Val Pro Ser Val Arg Asn 
        355                 360                 365             


Gly Asn Glu Ile Ala Ala Cys Leu Thr Lys Ala Gly Lys Arg Val Ile 
    370                 375                 380                 


Gln Leu Ser Arg Lys Thr Phe Glu Thr Glu Phe Gln Lys Thr Lys His 
385                 390                 395                 400 


Gln Glu Trp Asp Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala 
                405                 410                 415     


Asn Phe Lys Ala Asp Arg Val Ile Asp Ser Arg Arg Cys Leu Lys Pro 
            420                 425                 430         


Val Ile Leu Asp Gly Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val 
        435                 440                 445             


Thr His Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro 
    450                 455                 460                 


Asn Lys Pro Gly Asp Glu Tyr Leu Tyr Gly Gly Gly Cys Ala Glu Thr 
465                 470                 475                 480 


Asp Glu Asp His Ala His Trp Leu Glu Ala Arg Met Leu Leu Asp Asn 
                485                 490                 495     


Ile Tyr Leu Gln Asp Gly Leu Ile Ala Ser Leu Tyr Arg Pro Glu Ala 
            500                 505                 510         


Asp Lys Val Ala Ala Ile Glu Gly Glu Phe Lys Leu Arg Thr Glu Gln 
        515                 520                 525             


Arg Lys Thr Phe Val Glu Leu Met Lys Arg Gly Asp Leu Pro Val Trp 
    530                 535                 540                 


Leu Ala Tyr Gln Val Ala Ser Ala Gly Ile Thr Tyr Thr Asp Arg Arg 
545                 550                 555                 560 


Trp Cys Phe Asp Gly Thr Thr Asn Asn Thr Ile Met Glu Asp Ser Val 
                565                 570                 575     


Pro Ala Glu Val Trp Thr Arg His Gly Glu Lys Arg Val Leu Lys Pro 
            580                 585                 590         


Arg Trp Met Asp Ala Arg Val Cys Ser Asp His Ala Ala Leu Lys Ser 
        595                 600                 605             


Phe Lys Glu Phe Ala Ala Gly Lys Arg 
    610                 615         


<210>  30
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS4A

<400>  30

Gly Ala Ala Phe Gly Val Met Glu Ala Leu Gly Thr Leu Pro Gly His 
1               5                   10                  15      


Met Thr Glu Arg Phe Gln Glu Ala Ile Asp Asn Leu Ala Val Leu Met 
            20                  25                  30          


Arg Ala Glu Thr Gly Ser Arg Pro Tyr Lys Ala Ala Ala Ala Gln Leu 
        35                  40                  45              


Pro Glu Thr Leu Glu Thr Ile Met Leu Leu Gly Leu Leu Gly Thr Val 
    50                  55                  60                  


Ser Leu Gly Ile Phe Phe Val Leu Met Arg Asn Lys Gly Ile Gly Lys 
65                  70                  75                  80  


Met Gly Phe Gly Met Val Thr Leu Gly Ala Ser Ala Trp Leu Met Trp 
                85                  90                  95      


Leu Ser Glu Ile Glu Pro Ala Arg Ile Ala Cys Val Leu Ile Val Val 
            100                 105                 110         


Phe Leu Leu Leu Val Val Leu Ile Pro Glu Pro Glu Lys Gln Arg Ser 
        115                 120                 125             


Pro Gln Asp Asn Gln Met Ala Ile Ile Ile Met Val Ala Val Gly Leu 
    130                 135                 140                 


Leu Gly Leu Ile Thr Ala 
145                 150 


<210>  31
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS4B

<400>  31

Asn Glu Leu Gly Trp Leu Glu Arg Thr Lys Ser Asp Leu Ser His Leu 
1               5                   10                  15      


Met Gly Arg Arg Glu Glu Gly Ala Thr Ile Gly Phe Ser Met Asp Ile 
            20                  25                  30          


Asp Leu Arg Pro Ala Ser Ala Trp Ala Ile Tyr Ala Ala Leu Thr Thr 
        35                  40                  45              


Phe Ile Thr Pro Ala Val Gln His Ala Val Thr Thr Ser Tyr Asn Asn 
    50                  55                  60                  


Tyr Ser Leu Met Ala Met Ala Thr Gln Ala Gly Val Leu Phe Gly Met 
65                  70                  75                  80  


Gly Lys Gly Met Pro Phe Tyr Ala Trp Asp Phe Gly Val Pro Leu Leu 
                85                  90                  95      


Met Ile Gly Cys Tyr Ser Gln Leu Thr Pro Leu Thr Leu Ile Val Ala 
            100                 105                 110         


Ile Ile Leu Leu Val Ala His Tyr Met Tyr Leu Ile Pro Gly Leu Gln 
        115                 120                 125             


Ala Ala Ala Ala Arg Ala Ala Gln Lys Arg Thr Ala Ala Gly Ile Met 
    130                 135                 140                 


Lys Asn Pro Val Val Asp Gly Ile Val Val Thr Asp Ile Asp Thr Met 
145                 150                 155                 160 


Thr Ile Asp Pro Gln Val Glu Lys Lys Met Gly Gln Val Leu Leu Ile 
                165                 170                 175     


Ala Val Ala Val Ser Ser Ala Ile Leu Ser Arg Thr Ala Trp Gly Trp 
            180                 185                 190         


Gly Glu Ala Gly Ala Leu Ile Thr Ala Ala Thr Ser Thr Leu Trp Glu 
        195                 200                 205             


Gly Ser Pro Asn Lys Tyr Trp Asn Ser Ser Thr Ala Thr Ser Leu Cys 
    210                 215                 220                 


Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Ser Leu Ile Tyr Thr 
225                 230                 235                 240 


Val Thr Arg Asn Ala Gly Leu Val Lys Arg Arg 
                245                 250     


<210>  32
<211>  903
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS5

<400>  32

Gly Gly Gly Thr Gly Glu Thr Leu Gly Glu Lys Trp Lys Ala Arg Leu 
1               5                   10                  15      


Asn Gln Met Ser Ala Leu Glu Phe Tyr Ser Tyr Lys Lys Ser Gly Ile 
            20                  25                  30          


Thr Glu Val Cys Arg Glu Glu Ala Arg Arg Ala Leu Lys Asp Gly Val 
        35                  40                  45              


Ala Thr Gly Gly His Ala Val Ser Arg Gly Ser Ala Lys Leu Arg Trp 
    50                  55                  60                  


Leu Val Glu Arg Gly Tyr Leu Gln Pro Tyr Gly Lys Val Ile Asp Leu 
65                  70                  75                  80  


Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Ala Ala Thr Ile Arg Lys 
                85                  90                  95      


Val Gln Glu Val Lys Gly Tyr Thr Lys Gly Gly Pro Gly His Glu Glu 
            100                 105                 110         


Pro Val Leu Val Gln Ser Tyr Gly Trp Asn Ile Val Arg Leu Lys Ser 
        115                 120                 125             


Gly Val Asp Val Phe His Met Ala Ala Glu Pro Cys Asp Thr Leu Leu 
    130                 135                 140                 


Cys Asp Ile Gly Glu Ser Ser Ser Ser Pro Glu Val Glu Glu Ala Arg 
145                 150                 155                 160 


Thr Leu Arg Val Leu Ser Met Val Gly Asp Trp Leu Glu Lys Arg Pro 
                165                 170                 175     


Gly Ala Phe Cys Ile Lys Val Leu Cys Pro Tyr Thr Ser Thr Met Met 
            180                 185                 190         


Glu Thr Leu Glu Arg Leu Gln Arg Arg Tyr Gly Gly Gly Leu Val Arg 
        195                 200                 205             


Val Pro Leu Ser Arg Asn Ser Thr His Glu Met Tyr Trp Val Ser Gly 
    210                 215                 220                 


Ala Lys Ser Asn Thr Ile Lys Ser Val Ser Thr Thr Ser Gln Leu Leu 
225                 230                 235                 240 


Leu Gly Arg Met Asp Gly Pro Arg Arg Pro Val Lys Tyr Glu Glu Asp 
                245                 250                 255     


Val Asn Leu Gly Ser Gly Thr Arg Ala Val Val Ser Cys Ala Glu Ala 
            260                 265                 270         


Pro Asn Met Lys Ile Ile Gly Asn Arg Ile Glu Arg Ile Arg Ser Glu 
        275                 280                 285             


His Ala Glu Thr Trp Phe Phe Asp Glu Asn His Pro Tyr Arg Thr Trp 
    290                 295                 300                 


Ala Tyr His Gly Ser Tyr Glu Ala Pro Thr Gln Gly Ser Ala Ser Ser 
305                 310                 315                 320 


Leu Ile Asn Gly Val Val Arg Leu Leu Ser Lys Pro Trp Asp Val Val 
                325                 330                 335     


Thr Gly Val Thr Gly Ile Ala Met Thr Asp Thr Thr Pro Tyr Gly Gln 
            340                 345                 350         


Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Val Pro Asp Pro Gln 
        355                 360                 365             


Glu Gly Thr Arg Gln Val Met Ser Met Val Ser Ser Trp Leu Trp Lys 
    370                 375                 380                 


Glu Leu Gly Lys His Lys Arg Pro Arg Val Cys Thr Lys Glu Glu Phe 
385                 390                 395                 400 


Ile Asn Lys Val Arg Ser Asn Ala Ala Leu Gly Ala Ile Phe Glu Glu 
                405                 410                 415     


Glu Lys Glu Trp Lys Thr Ala Val Glu Ala Val Asn Asp Pro Arg Phe 
            420                 425                 430         


Trp Ala Leu Val Asp Lys Glu Arg Glu His His Leu Arg Gly Glu Cys 
        435                 440                 445             


Gln Ser Cys Val Tyr Asn Met Met Gly Lys Arg Glu Lys Lys Gln Gly 
    450                 455                 460                 


Glu Phe Gly Lys Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu 
465                 470                 475                 480 


Gly Ala Arg Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp 
                485                 490                 495     


His Trp Met Gly Arg Glu Asn Ser Gly Gly Gly Val Glu Gly Leu Gly 
            500                 505                 510         


Leu Gln Arg Leu Gly Tyr Val Leu Glu Glu Met Ser Arg Ile Pro Gly 
        515                 520                 525             


Gly Arg Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Ser 
    530                 535                 540                 


Arg Phe Asp Leu Glu Asn Glu Ala Leu Ile Thr Asn Gln Met Glu Lys 
545                 550                 555                 560 


Gly His Arg Ala Leu Ala Leu Ala Ile Ile Lys Tyr Thr Tyr Gln Asn 
                565                 570                 575     


Lys Val Val Lys Val Leu Arg Pro Ala Glu Lys Gly Lys Thr Val Met 
            580                 585                 590         


Asp Ile Ile Ser Arg Gln Asp Gln Arg Gly Ser Gly Gln Val Val Thr 
        595                 600                 605             


Tyr Ala Leu Asn Thr Phe Thr Asn Leu Val Val Gln Leu Ile Arg Asn 
    610                 615                 620                 


Met Glu Ala Glu Glu Val Leu Glu Met Gln Asp Leu Trp Leu Leu Arg 
625                 630                 635                 640 


Arg Ser Glu Lys Val Thr Asn Trp Leu Gln Ser Asn Gly Trp Asp Arg 
                645                 650                 655     


Leu Lys Arg Met Ala Val Ser Gly Asp Asp Cys Val Val Lys Pro Ile 
            660                 665                 670         


Asp Asp Arg Phe Ala His Ala Leu Arg Phe Leu Asn Asp Met Gly Lys 
        675                 680                 685             


Val Arg Lys Asp Thr Gln Glu Trp Lys Pro Ser Thr Gly Trp Asp Asn 
    690                 695                 700                 


Trp Glu Glu Val Pro Phe Cys Ser His His Phe Asn Lys Leu His Leu 
705                 710                 715                 720 


Lys Asp Gly Arg Ser Ile Val Val Pro Cys Arg His Gln Asp Glu Leu 
                725                 730                 735     


Ile Gly Arg Ala Arg Val Ser Pro Gly Ala Gly Trp Ser Ile Arg Glu 
            740                 745                 750         


Thr Ala Cys Leu Ala Lys Ser Tyr Ala Gln Met Trp Gln Leu Leu Tyr 
        755                 760                 765             


Phe His Arg Arg Asp Leu Arg Leu Met Ala Asn Ala Ile Cys Ser Ser 
    770                 775                 780                 


Val Pro Val Asp Trp Val Pro Thr Gly Arg Thr Thr Trp Ser Ile His 
785                 790                 795                 800 


Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Val Val Trp Asn 
                805                 810                 815     


Arg Val Trp Ile Glu Glu Asn Asp His Met Glu Asp Lys Thr Pro Val 
            820                 825                 830         


Thr Lys Trp Thr Asp Ile Pro Tyr Leu Gly Lys Arg Glu Asp Leu Trp 
        835                 840                 845             


Cys Gly Ser Leu Ile Gly His Arg Pro Arg Thr Thr Trp Ala Glu Asn 
    850                 855                 860                 


Ile Lys Asn Thr Val Asn Met Val Arg Arg Ile Ile Gly Asp Glu Glu 
865                 870                 875                 880 


Lys Tyr Met Asp Tyr Leu Ser Thr Gln Val Arg Tyr Leu Gly Glu Glu 
                885                 890                 895     


Gly Ser Thr Pro Gly Val Leu 
            900             


<210>  33
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804)

<400>  33

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  34
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723)

<400>  34

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  35
<211>  3419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein

<400>  35

Met Lys Asn Pro Lys Glu Glu Ile Arg Arg Ile Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Asn Pro Leu Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr Ser Ile Gly Ile 
            100                 105                 110         


Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Ile Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp Ala Gly Lys Ala 
    130                 135                 140                 


Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys His Val Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu 
        435                 440                 445             


Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala 
    450                 455                 460                 


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
465                 470                 475                 480 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
                485                 490                 495     


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
            500                 505                 510         


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
        515                 520                 525             


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
    530                 535                 540                 


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
545                 550                 555                 560 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys 
                565                 570                 575     


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
            580                 585                 590         


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu 
        595                 600                 605             


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
    610                 615                 620                 


Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
625                 630                 635                 640 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
                645                 650                 655     


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
            660                 665                 670         


Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg 
        675                 680                 685             


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
    690                 695                 700                 


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
705                 710                 715                 720 


Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
                725                 730                 735     


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
            740                 745                 750         


Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly 
        755                 760                 765             


Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu 
    770                 775                 780                 


Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
785                 790                 795                 800 


Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Ile Tyr Asn Asp Val Glu 
                805                 810                 815     


Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu 
            820                 825                 830         


Ala Ala Ala Val Lys Gln Ala Trp Glu Glu Gly Ile Cys Gly Ile Ser 
        835                 840                 845             


Ser Val Ser Arg Met Glu Asn Ile Met Trp Lys Ser Val Glu Gly Glu 
    850                 855                 860                 


Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val 
865                 870                 875                 880 


Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val 
                885                 890                 895     


Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser Tyr 
            900                 905                 910         


Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp 
        915                 920                 925             


Thr Leu Lys Glu Cys Pro Leu Glu His Arg Ala Trp Asn Ser Phe Leu 
    930                 935                 940                 


Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys 
945                 950                 955                 960 


Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr 
                965                 970                 975     


Ala Val Lys Gly Arg Glu Ala Ala His Ser Asp Leu Gly Tyr Trp Ile 
            980                 985                 990         


Glu Ser Glu Lys Asn Asp Thr Trp  Arg Leu Lys Arg Ala  His Leu Ile 
        995                 1000                 1005             


Glu Met  Lys Thr Cys Glu Trp  Pro Lys Ser His Thr  Leu Trp Thr 
    1010                 1015                 1020             


Asp Gly  Val Glu Glu Ser Asp  Leu Ile Ile Pro Lys  Ser Leu Ala 
    1025                 1030                 1035             


Gly Pro  Leu Ser His His Asn  Thr Arg Glu Gly Tyr  Arg Thr Gln 
    1040                 1045                 1050             


Val Lys  Gly Pro Trp His Ser  Glu Glu Leu Glu Ile  Arg Phe Glu 
    1055                 1060                 1065             


Glu Cys  Pro Gly Thr Lys Val  Tyr Val Glu Glu Thr  Cys Gly Thr 
    1070                 1075                 1080             


Arg Gly  Pro Ser Leu Arg Ser  Thr Thr Ala Ser Gly  Arg Val Ile 
    1085                 1090                 1095             


Glu Glu  Trp Cys Cys Arg Glu  Cys Thr Met Pro Pro  Leu Ser Phe 
    1100                 1105                 1110             


Arg Ala  Lys Asp Gly Cys Trp  Tyr Gly Met Glu Ile  Arg Pro Arg 
    1115                 1120                 1125             


Lys Glu  Pro Glu Ser Asn Leu  Val Arg Ser Met Val  Thr Ala Gly 
    1130                 1135                 1140             


Ser Thr  Asp His Met Asp His  Phe Ser Leu Gly Val  Leu Val Ile 
    1145                 1150                 1155             


Leu Leu  Met Val Gln Glu Gly  Leu Lys Lys Arg Met  Thr Thr Lys 
    1160                 1165                 1170             


Ile Ile  Met Ser Thr Ser Met  Ala Val Leu Val Val  Met Ile Leu 
    1175                 1180                 1185             


Gly Gly  Phe Ser Met Ser Asp  Leu Ala Lys Leu Val  Ile Leu Met 
    1190                 1195                 1200             


Gly Ala  Thr Phe Ala Glu Met  Asn Thr Gly Gly Asp  Val Ala His 
    1205                 1210                 1215             


Leu Ala  Leu Val Ala Ala Phe  Lys Val Arg Pro Ala  Leu Leu Val 
    1220                 1225                 1230             


Ser Phe  Ile Phe Arg Ala Asn  Trp Thr Pro Arg Glu  Ser Met Leu 
    1235                 1240                 1245             


Leu Ala  Leu Ala Ser Cys Leu  Leu Gln Thr Ala Ile  Ser Ala Leu 
    1250                 1255                 1260             


Glu Gly  Asp Leu Met Val Leu  Ile Asn Gly Phe Ala  Leu Ala Trp 
    1265                 1270                 1275             


Leu Ala  Ile Arg Ala Met Ala  Val Pro Arg Thr Asp  Asn Ile Ala 
    1280                 1285                 1290             


Leu Pro  Ile Leu Ala Ala Leu  Thr Pro Leu Ala Arg  Gly Thr Leu 
    1295                 1300                 1305             


Leu Val  Ala Trp Arg Ala Gly  Leu Ala Thr Cys Gly  Gly Ile Met 
    1310                 1315                 1320             


Leu Leu  Ser Leu Lys Gly Lys  Gly Ser Val Lys Lys  Asn Leu Pro 
    1325                 1330                 1335             


Phe Val  Met Ala Leu Gly Leu  Thr Ala Val Arg Val  Val Asp Pro 
    1340                 1345                 1350             


Ile Asn  Val Val Gly Leu Leu  Leu Leu Thr Arg Ser  Gly Lys Arg 
    1355                 1360                 1365             


Ser Trp  Pro Pro Ser Glu Val  Leu Thr Ala Val Gly  Leu Ile Cys 
    1370                 1375                 1380             


Ala Leu  Ala Gly Gly Phe Ala  Lys Ala Asp Ile Glu  Met Ala Gly 
    1385                 1390                 1395             


Pro Met  Ala Ala Val Gly Leu  Leu Ile Val Ser Tyr  Val Val Ser 
    1400                 1405                 1410             


Gly Lys  Ser Val Asp Met Tyr  Ile Glu Arg Ala Gly  Asp Ile Thr 
    1415                 1420                 1425             


Trp Glu  Lys Asp Ala Glu Val  Thr Gly Asn Ser Pro  Arg Leu Asp 
    1430                 1435                 1440             


Val Ala  Leu Asp Glu Ser Gly  Asp Phe Ser Leu Val  Glu Glu Asp 
    1445                 1450                 1455             


Gly Pro  Pro Met Arg Glu Ile  Ile Leu Lys Val Val  Leu Met Ala 
    1460                 1465                 1470             


Ile Cys  Gly Met Asn Pro Ile  Ala Ile Pro Phe Ala  Ala Gly Ala 
    1475                 1480                 1485             


Trp Tyr  Val Tyr Val Lys Thr  Gly Lys Arg Ser Gly  Ala Leu Trp 
    1490                 1495                 1500             


Asp Val  Pro Ala Pro Lys Glu  Val Lys Lys Gly Glu  Thr Thr Asp 
    1505                 1510                 1515             


Gly Val  Tyr Arg Val Met Thr  Arg Arg Leu Leu Gly  Ser Thr Gln 
    1520                 1525                 1530             


Val Gly  Val Gly Val Met Gln  Glu Gly Val Phe His  Thr Met Trp 
    1535                 1540                 1545             


His Val  Thr Lys Gly Ala Ala  Leu Arg Ser Gly Glu  Gly Arg Leu 
    1550                 1555                 1560             


Asp Pro  Tyr Trp Gly Asp Val  Lys Gln Asp Leu Val  Ser Tyr Cys 
    1565                 1570                 1575             


Gly Pro  Trp Lys Leu Asp Ala  Ala Trp Asp Gly Leu  Ser Glu Val 
    1580                 1585                 1590             


Gln Leu  Leu Ala Val Pro Pro  Gly Glu Arg Ala Arg  Asn Ile Gln 
    1595                 1600                 1605             


Thr Leu  Pro Gly Ile Phe Lys  Thr Lys Asp Gly Asp  Ile Gly Ala 
    1610                 1615                 1620             


Val Ala  Leu Asp Tyr Pro Ala  Gly Thr Ser Gly Ser  Pro Ile Leu 
    1625                 1630                 1635             


Asp Lys  Cys Gly Arg Val Ile  Gly Leu Tyr Gly Asn  Gly Val Val 
    1640                 1645                 1650             


Ile Lys  Asn Gly Ser Tyr Val  Ser Ala Ile Thr Gln  Gly Lys Arg 
    1655                 1660                 1665             


Glu Glu  Glu Thr Pro Val Glu  Cys Phe Glu Pro Ser  Met Leu Lys 
    1670                 1675                 1680             


Lys Lys  Gln Leu Thr Val Leu  Asp Leu His Pro Gly  Ala Gly Lys 
    1685                 1690                 1695             


Thr Arg  Arg Val Leu Pro Glu  Ile Val Arg Glu Ala  Ile Lys Lys 
    1700                 1705                 1710             


Arg Leu  Arg Thr Val Ile Leu  Ala Pro Thr Arg Val  Val Ala Ala 
    1715                 1720                 1725             


Glu Met  Glu Glu Ala Leu Arg  Gly Leu Pro Val Arg  Tyr Met Thr 
    1730                 1735                 1740             


Thr Ala  Val Asn Val Thr His  Ser Gly Thr Glu Ile  Val Asp Leu 
    1745                 1750                 1755             


Met Cys  His Ala Thr Phe Thr  Ser Arg Leu Leu Gln  Pro Ile Arg 
    1760                 1765                 1770             


Val Pro  Asn Tyr Asn Leu Asn  Ile Met Asp Glu Ala  His Phe Thr 
    1775                 1780                 1785             


Asp Pro  Ser Ser Ile Ala Ala  Arg Gly Tyr Ile Ser  Thr Arg Val 
    1790                 1795                 1800             


Glu Met  Gly Glu Ala Ala Ala  Ile Phe Met Thr Ala  Thr Pro Pro 
    1805                 1810                 1815             


Gly Thr  Arg Asp Ala Phe Pro  Asp Ser Asn Ser Pro  Ile Met Asp 
    1820                 1825                 1830             


Thr Glu  Val Glu Val Pro Glu  Arg Ala Trp Ser Ser  Gly Phe Asp 
    1835                 1840                 1845             


Trp Val  Thr Asp His Ser Gly  Lys Thr Val Trp Phe  Val Pro Ser 
    1850                 1855                 1860             


Val Arg  Asn Gly Asn Glu Ile  Ala Ala Cys Leu Thr  Lys Ala Gly 
    1865                 1870                 1875             


Lys Arg  Val Ile Gln Leu Ser  Arg Lys Thr Phe Glu  Thr Glu Phe 
    1880                 1885                 1890             


Gln Lys  Thr Lys Asn Gln Glu  Trp Asp Phe Val Ile  Thr Thr Asp 
    1895                 1900                 1905             


Ile Ser  Glu Met Gly Ala Asn  Phe Lys Ala Asp Arg  Val Ile Asp 
    1910                 1915                 1920             


Ser Arg  Arg Cys Leu Lys Pro  Val Ile Leu Asp Gly  Glu Arg Val 
    1925                 1930                 1935             


Ile Leu  Ala Gly Pro Met Pro  Val Thr His Ala Ser  Ala Ala Gln 
    1940                 1945                 1950             


Arg Arg  Gly Arg Ile Gly Arg  Asn Pro Asn Lys Pro  Gly Asp Glu 
    1955                 1960                 1965             


Tyr Met  Tyr Gly Gly Gly Cys  Ala Glu Thr Asp Glu  Gly His Ala 
    1970                 1975                 1980             


His Trp  Leu Glu Ala Arg Met  Leu Leu Asp Asn Ile  Tyr Leu Gln 
    1985                 1990                 1995             


Asp Gly  Leu Ile Ala Ser Leu  Tyr Arg Pro Glu Ala  Asp Lys Val 
    2000                 2005                 2010             


Ala Ala  Ile Glu Gly Glu Phe  Lys Leu Arg Thr Glu  Gln Arg Lys 
    2015                 2020                 2025             


Thr Phe  Val Glu Leu Met Lys  Arg Gly Asp Leu Pro  Val Trp Leu 
    2030                 2035                 2040             


Ala Tyr  Gln Val Ala Ser Ala  Gly Ile Thr Tyr Thr  Asp Arg Arg 
    2045                 2050                 2055             


Trp Cys  Phe Asp Gly Thr Thr  Asn Asn Thr Ile Met  Glu Asp Ser 
    2060                 2065                 2070             


Val Pro  Ala Glu Val Trp Thr  Lys Tyr Gly Glu Lys  Arg Val Leu 
    2075                 2080                 2085             


Lys Pro  Arg Trp Met Asp Ala  Arg Val Cys Ser Asp  His Ala Ala 
    2090                 2095                 2100             


Leu Lys  Ser Phe Lys Glu Phe  Ala Ala Gly Lys Arg  Gly Ala Ala 
    2105                 2110                 2115             


Leu Gly  Val Met Glu Ala Leu  Gly Thr Leu Pro Gly  His Met Thr 
    2120                 2125                 2130             


Glu Arg  Phe Gln Glu Ala Ile  Asp Asn Leu Ala Val  Leu Met Arg 
    2135                 2140                 2145             


Ala Glu  Thr Gly Ser Arg Pro  Tyr Lys Ala Ala Ala  Ala Gln Leu 
    2150                 2155                 2160             


Pro Glu  Thr Leu Glu Thr Ile  Met Leu Leu Gly Leu  Leu Gly Thr 
    2165                 2170                 2175             


Val Ser  Leu Gly Ile Phe Phe  Val Leu Met Arg Asn  Lys Gly Ile 
    2180                 2185                 2190             


Gly Lys  Met Gly Phe Gly Met  Val Thr Leu Gly Ala  Ser Ala Trp 
    2195                 2200                 2205             


Leu Met  Trp Leu Ser Glu Ile  Glu Pro Ala Arg Ile  Ala Cys Val 
    2210                 2215                 2220             


Leu Ile  Val Val Phe Leu Leu  Leu Val Val Leu Ile  Pro Glu Pro 
    2225                 2230                 2235             


Glu Lys  Gln Arg Ser Pro Gln  Asp Asn Gln Met Ala  Ile Ile Ile 
    2240                 2245                 2250             


Met Val  Ala Val Gly Leu Leu  Gly Leu Ile Thr Ala  Asn Glu Leu 
    2255                 2260                 2265             


Gly Trp  Leu Glu Arg Thr Lys  Asn Asp Ile Ala His  Leu Met Gly 
    2270                 2275                 2280             


Arg Arg  Glu Glu Gly Ala Thr  Met Gly Phe Ser Met  Asp Ile Asp 
    2285                 2290                 2295             


Leu Arg  Pro Ala Ser Ala Trp  Ala Ile Tyr Ala Ala  Leu Thr Thr 
    2300                 2305                 2310             


Leu Ile  Thr Pro Ala Val Gln  His Ala Val Thr Thr  Ser Tyr Asn 
    2315                 2320                 2325             


Asn Tyr  Ser Leu Met Ala Met  Ala Thr Gln Ala Gly  Val Leu Phe 
    2330                 2335                 2340             


Gly Met  Gly Lys Gly Met Pro  Phe Met His Gly Asp  Leu Gly Val 
    2345                 2350                 2355             


Pro Leu  Leu Met Met Gly Cys  Tyr Ser Gln Leu Thr  Pro Leu Thr 
    2360                 2365                 2370             


Leu Ile  Val Ala Ile Ile Leu  Leu Val Ala His Tyr  Met Tyr Leu 
    2375                 2380                 2385             


Ile Pro  Gly Leu Gln Ala Ala  Ala Ala Arg Ala Ala  Gln Lys Arg 
    2390                 2395                 2400             


Thr Ala  Ala Gly Ile Met Lys  Asn Pro Val Val Asp  Gly Ile Val 
    2405                 2410                 2415             


Val Thr  Asp Ile Asp Thr Met  Thr Ile Asp Pro Gln  Val Glu Lys 
    2420                 2425                 2430             


Lys Met  Gly Gln Val Leu Leu  Ile Ala Val Ala Ile  Ser Ser Ala 
    2435                 2440                 2445             


Val Leu  Leu Arg Thr Ala Trp  Gly Trp Gly Glu Ala  Gly Ala Leu 
    2450                 2455                 2460             


Ile Thr  Ala Ala Thr Ser Thr  Leu Trp Glu Gly Ser  Pro Asn Lys 
    2465                 2470                 2475             


Tyr Trp  Asn Ser Ser Thr Ala  Thr Ser Leu Cys Asn  Ile Phe Arg 
    2480                 2485                 2490             


Gly Ser  Tyr Leu Ala Gly Ala  Ser Leu Ile Tyr Thr  Val Thr Arg 
    2495                 2500                 2505             


Asn Ala  Gly Leu Val Lys Arg  Arg Gly Gly Gly Thr  Gly Glu Thr 
    2510                 2515                 2520             


Leu Gly  Glu Lys Trp Lys Ala  Arg Leu Asn Gln Met  Ser Ala Leu 
    2525                 2530                 2535             


Glu Phe  Tyr Ser Tyr Lys Lys  Ser Gly Ile Thr Glu  Val Cys Arg 
    2540                 2545                 2550             


Glu Glu  Ala Arg Arg Ala Leu  Lys Asp Gly Val Ala  Thr Gly Gly 
    2555                 2560                 2565             


His Ala  Val Ser Arg Gly Ser  Ala Lys Ile Arg Trp  Leu Glu Glu 
    2570                 2575                 2580             


Arg Gly  Tyr Leu Gln Pro Tyr  Gly Lys Val Val Asp  Leu Gly Cys 
    2585                 2590                 2595             


Gly Arg  Gly Gly Trp Ser Tyr  Tyr Ala Ala Thr Ile  Arg Lys Val 
    2600                 2605                 2610             


Gln Glu  Val Arg Gly Tyr Thr  Lys Gly Gly Pro Gly  His Glu Glu 
    2615                 2620                 2625             


Pro Met  Leu Val Gln Ser Tyr  Gly Trp Asn Ile Val  Arg Leu Lys 
    2630                 2635                 2640             


Ser Gly  Val Asp Val Phe His  Met Ala Ala Glu Pro  Cys Asp Thr 
    2645                 2650                 2655             


Leu Leu  Cys Asp Ile Gly Glu  Ser Ser Ser Ser Pro  Glu Val Glu 
    2660                 2665                 2670             


Glu Thr  Arg Thr Leu Arg Val  Leu Ser Met Val Gly  Asp Trp Leu 
    2675                 2680                 2685             


Glu Lys  Arg Pro Gly Ala Phe  Cys Ile Lys Val Leu  Cys Pro Tyr 
    2690                 2695                 2700             


Thr Ser  Thr Met Met Glu Thr  Met Glu Arg Leu Gln  Arg Arg His 
    2705                 2710                 2715             


Gly Gly  Gly Leu Val Arg Val  Pro Leu Cys Arg Asn  Ser Thr His 
    2720                 2725                 2730             


Glu Met  Tyr Trp Val Ser Gly  Ala Lys Ser Asn Ile  Ile Lys Ser 
    2735                 2740                 2745             


Val Ser  Thr Thr Ser Gln Leu  Leu Leu Gly Arg Met  Asp Gly Pro 
    2750                 2755                 2760             


Arg Arg  Pro Val Lys Tyr Glu  Glu Asp Val Asn Leu  Gly Ser Gly 
    2765                 2770                 2775             


Thr Arg  Ala Val Ala Ser Cys  Ala Glu Ala Pro Asn  Met Lys Ile 
    2780                 2785                 2790             


Ile Gly  Arg Arg Ile Glu Arg  Ile Arg Asn Glu His  Ala Glu Thr 
    2795                 2800                 2805             


Trp Phe  Leu Asp Glu Asn His  Pro Tyr Arg Thr Trp  Ala Tyr His 
    2810                 2815                 2820             


Gly Ser  Tyr Glu Ala Pro Thr  Gln Gly Ser Ala Ser  Ser Leu Val 
    2825                 2830                 2835             


Asn Gly  Val Val Arg Leu Leu  Ser Lys Pro Trp Asp  Val Val Thr 
    2840                 2845                 2850             


Gly Val  Thr Gly Ile Ala Met  Thr Asp Thr Thr Pro  Tyr Gly Gln 
    2855                 2860                 2865             


Gln Arg  Val Phe Lys Glu Lys  Val Asp Thr Arg Val  Pro Asp Pro 
    2870                 2875                 2880             


Gln Glu  Gly Thr Arg Gln Val  Met Asn Ile Val Ser  Ser Trp Leu 
    2885                 2890                 2895             


Trp Lys  Glu Leu Gly Lys Arg  Lys Arg Pro Arg Val  Cys Thr Lys 
    2900                 2905                 2910             


Glu Glu  Phe Ile Asn Lys Val  Arg Ser Asn Ala Ala  Leu Gly Ala 
    2915                 2920                 2925             


Ile Phe  Glu Glu Glu Lys Glu  Trp Lys Thr Ala Val  Glu Ala Val 
    2930                 2935                 2940             


Asn Asp  Pro Arg Phe Trp Ala  Leu Val Asp Arg Glu  Arg Glu His 
    2945                 2950                 2955             


His Leu  Arg Gly Glu Cys His  Ser Cys Val Tyr Asn  Met Met Gly 
    2960                 2965                 2970             


Lys Arg  Glu Lys Lys Gln Gly  Glu Phe Gly Lys Ala  Lys Gly Ser 
    2975                 2980                 2985             


Arg Ala  Ile Trp Tyr Met Trp  Leu Gly Ala Arg Phe  Leu Glu Phe 
    2990                 2995                 3000             


Glu Ala  Leu Gly Phe Leu Asn  Glu Asp His Trp Met  Gly Arg Glu 
    3005                 3010                 3015             


Asn Ser  Gly Gly Gly Val Glu  Gly Leu Gly Leu Gln  Arg Leu Gly 
    3020                 3025                 3030             


Tyr Ile  Leu Glu Glu Met Asn  Arg Ala Pro Gly Gly  Lys Met Tyr 
    3035                 3040                 3045             


Ala Asp  Asp Thr Ala Gly Trp  Asp Thr Arg Ile Ser  Lys Phe Asp 
    3050                 3055                 3060             


Leu Glu  Asn Glu Ala Leu Ile  Thr Asn Gln Met Glu  Glu Gly His 
    3065                 3070                 3075             


Arg Thr  Leu Ala Leu Ala Val  Ile Lys Tyr Thr Tyr  Gln Asn Lys 
    3080                 3085                 3090             


Val Val  Lys Val Leu Arg Pro  Ala Glu Gly Gly Lys  Thr Val Met 
    3095                 3100                 3105             


Asp Ile  Ile Ser Arg Gln Asp  Gln Arg Gly Ser Gly  Gln Val Val 
    3110                 3115                 3120             


Thr Tyr  Ala Leu Asn Thr Phe  Thr Asn Leu Val Val  Gln Leu Ile 
    3125                 3130                 3135             


Arg Asn  Met Glu Ala Glu Glu  Val Leu Glu Met Gln  Asp Leu Trp 
    3140                 3145                 3150             


Leu Leu  Arg Lys Pro Glu Lys  Val Thr Arg Trp Leu  Gln Ser Asn 
    3155                 3160                 3165             


Gly Trp  Asp Arg Leu Lys Arg  Met Ala Val Ser Gly  Asp Asp Cys 
    3170                 3175                 3180             


Val Val  Lys Pro Ile Asp Asp  Arg Phe Ala His Ala  Leu Arg Phe 
    3185                 3190                 3195             


Leu Asn  Asp Met Gly Lys Val  Arg Lys Asp Thr Gln  Glu Trp Lys 
    3200                 3205                 3210             


Pro Ser  Thr Gly Trp Ser Asn  Trp Glu Glu Val Pro  Phe Cys Ser 
    3215                 3220                 3225             


His His  Phe Asn Lys Leu Tyr  Leu Lys Asp Gly Arg  Ser Ile Val 
    3230                 3235                 3240             


Val Pro  Cys Arg His Gln Asp  Glu Leu Ile Gly Arg  Ala Arg Val 
    3245                 3250                 3255             


Ser Pro  Gly Ala Gly Trp Ser  Ile Arg Glu Thr Ala  Cys Leu Ala 
    3260                 3265                 3270             


Lys Ser  Tyr Ala Gln Met Trp  Gln Leu Leu Tyr Phe  His Arg Arg 
    3275                 3280                 3285             


Asp Leu  Arg Leu Met Ala Asn  Ala Ile Cys Ser Ala  Val Pro Val 
    3290                 3295                 3300             


Asp Trp  Val Pro Thr Gly Arg  Thr Thr Trp Ser Ile  His Gly Lys 
    3305                 3310                 3315             


Gly Glu  Trp Met Thr Thr Glu  Asp Met Leu Met Val  Trp Asn Arg 
    3320                 3325                 3330             


Val Trp  Ile Glu Glu Asn Asp  His Met Glu Asp Lys  Thr Pro Val 
    3335                 3340                 3345             


Thr Lys  Trp Thr Asp Ile Pro  Tyr Leu Gly Lys Arg  Glu Asp Leu 
    3350                 3355                 3360             


Trp Cys  Gly Ser Leu Ile Gly  His Arg Pro Arg Thr  Thr Trp Ala 
    3365                 3370                 3375             


Glu Asn  Ile Lys Asp Thr Val  Asn Met Val Arg Arg  Ile Ile Gly 
    3380                 3385                 3390             


Asp Glu  Glu Lys Tyr Met Asp  Tyr Leu Ser Thr Gln  Val Arg Tyr 
    3395                 3400                 3405             


Leu Gly  Glu Glu Gly Ser Thr  Pro Gly Val Leu 
    3410                 3415                 


<210>  36
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C

<400>  36

Met Lys Asn Pro Lys Glu Glu Ile Arg Arg Ile Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Asn Pro Leu Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Arg Lys Arg Arg 
            100                 


<210>  37
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-ER anchor

<400>  37

Met Lys Asn Pro Lys Glu Glu Ile Arg Arg Ile Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Asn Pro Leu Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr Ser Ile Gly Ile 
            100                 105                 110         


Ile Gly Leu Leu Leu Thr Thr Ala Met Ala 
        115                 120         


<210>  38
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) pr

<400>  38

Ala Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn 
            20                  25                  30          


Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
                85                  90              


<210>  39
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) M

<400>  39

Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
1               5                   10                  15      


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val 
            20                  25                  30          


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala 
        35                  40                  45              


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
    50                  55                  60                  


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
65                  70                  75  


<210>  40
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prM

<400>  40

Ala Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn 
            20                  25                  30          


Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
                85                  90                  95      


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
            100                 105                 110         


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp 
        115                 120                 125             


Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp 
    130                 135                 140                 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
145                 150                 155                 160 


Leu Leu Ile Ala Pro Ala Tyr Ser 
                165             


<210>  41
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) E

<400>  41

Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
1               5                   10                  15      


Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
            20                  25                  30          


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
        35                  40                  45              


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
    50                  55                  60                  


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
65                  70                  75                  80  


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
                85                  90                  95      


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
            100                 105                 110         


Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 
        115                 120                 125             


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
    130                 135                 140                 


Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg 
145                 150                 155                 160 


Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
                165                 170                 175     


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 
            180                 185                 190         


Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 
        195                 200                 205             


Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 
    210                 215                 220                 


Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu 
225                 230                 235                 240 


Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 
                245                 250                 255     


Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 
            260                 265                 270         


Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg 
        275                 280                 285             


Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 
    290                 295                 300                 


Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly 
305                 310                 315                 320 


Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 
                325                 330                 335     


Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 
            340                 345                 350         


Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 
        355                 360                 365             


Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 
    370                 375                 380                 


Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly 
385                 390                 395                 400 


Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 
                405                 410                 415     


Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly 
            420                 425                 430         


Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala 
        435                 440                 445             


Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile 
    450                 455                 460                 


Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile 
465                 470                 475                 480 


Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr 
                485                 490                 495     


Ala Val Ser Ala 
            500 


<210>  42
<211>  668
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME

<400>  42

Ala Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn 
            20                  25                  30          


Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
                85                  90                  95      


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
            100                 105                 110         


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp 
        115                 120                 125             


Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp 
    130                 135                 140                 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
145                 150                 155                 160 


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
                165                 170                 175     


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
            180                 185                 190         


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
        195                 200                 205             


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
    210                 215                 220                 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
225                 230                 235                 240 


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
                245                 250                 255     


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
            260                 265                 270         


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr 
        275                 280                 285             


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
    290                 295                 300                 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
305                 310                 315                 320 


Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn 
                325                 330                 335     


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
            340                 345                 350         


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
        355                 360                 365             


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
    370                 375                 380                 


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
385                 390                 395                 400 


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
                405                 410                 415     


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
            420                 425                 430         


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
        435                 440                 445             


Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
    450                 455                 460                 


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
465                 470                 475                 480 


Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
                485                 490                 495     


Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp 
            500                 505                 510         


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
        515                 520                 525             


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
    530                 535                 540                 


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile 
545                 550                 555                 560 


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
                565                 570                 575     


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
            580                 585                 590         


Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly 
        595                 600                 605             


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
    610                 615                 620                 


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly 
625                 630                 635                 640 


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly 
                645                 650                 655     


Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
            660                 665             


<210>  43
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS1

<400>  43

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Ile Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Glu Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu 
    50                  55                  60                  


Asn Ile Met Trp Lys Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu 
65                  70                  75                  80  


Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro 
                85                  90                  95      


Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro 
            100                 105                 110         


His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys 
        115                 120                 125             


Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro 
    130                 135                 140                 


Leu Glu His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe 
145                 150                 155                 160 


Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser 
                165                 170                 175     


Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Arg Glu 
            180                 185                 190         


Ala Ala His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp 
        195                 200                 205             


Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Val Glu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr 
                245                 250                 255     


Arg Glu Gly Tyr Arg Thr Gln Val Lys Gly Pro Trp His Ser Glu Glu 
            260                 265                 270         


Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val Tyr Val Glu 
        275                 280                 285             


Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 
    290                 295                 300                 


Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr Ala 
            340                 345                 350         


<210>  44
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS2A

<400>  44

Gly Ser Thr Asp His Met Asp His Phe Ser Leu Gly Val Leu Val Ile 
1               5                   10                  15      


Leu Leu Met Val Gln Glu Gly Leu Lys Lys Arg Met Thr Thr Lys Ile 
            20                  25                  30          


Ile Met Ser Thr Ser Met Ala Val Leu Val Val Met Ile Leu Gly Gly 
        35                  40                  45              


Phe Ser Met Ser Asp Leu Ala Lys Leu Val Ile Leu Met Gly Ala Thr 
    50                  55                  60                  


Phe Ala Glu Met Asn Thr Gly Gly Asp Val Ala His Leu Ala Leu Val 
65                  70                  75                  80  


Ala Ala Phe Lys Val Arg Pro Ala Leu Leu Val Ser Phe Ile Phe Arg 
                85                  90                  95      


Ala Asn Trp Thr Pro Arg Glu Ser Met Leu Leu Ala Leu Ala Ser Cys 
            100                 105                 110         


Leu Leu Gln Thr Ala Ile Ser Ala Leu Glu Gly Asp Leu Met Val Leu 
        115                 120                 125             


Ile Asn Gly Phe Ala Leu Ala Trp Leu Ala Ile Arg Ala Met Ala Val 
    130                 135                 140                 


Pro Arg Thr Asp Asn Ile Ala Leu Pro Ile Leu Ala Ala Leu Thr Pro 
145                 150                 155                 160 


Leu Ala Arg Gly Thr Leu Leu Val Ala Trp Arg Ala Gly Leu Ala Thr 
                165                 170                 175     


Cys Gly Gly Ile Met Leu Leu Ser Leu Lys Gly Lys Gly Ser Val Lys 
            180                 185                 190         


Lys Asn Leu Pro Phe Val Met Ala Leu Gly Leu Thr Ala Val Arg Val 
        195                 200                 205             


Val Asp Pro Ile Asn Val Val Gly Leu Leu Leu Leu Thr Arg Ser Gly 
    210                 215                 220                 


Lys Arg 
225     


<210>  45
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS2B

<400>  45

Ser Trp Pro Pro Ser Glu Val Leu Thr Ala Val Gly Leu Ile Cys Ala 
1               5                   10                  15      


Leu Ala Gly Gly Phe Ala Lys Ala Asp Ile Glu Met Ala Gly Pro Met 
            20                  25                  30          


Ala Ala Val Gly Leu Leu Ile Val Ser Tyr Val Val Ser Gly Lys Ser 
        35                  40                  45              


Val Asp Met Tyr Ile Glu Arg Ala Gly Asp Ile Thr Trp Glu Lys Asp 
    50                  55                  60                  


Ala Glu Val Thr Gly Asn Ser Pro Arg Leu Asp Val Ala Leu Asp Glu 
65                  70                  75                  80  


Ser Gly Asp Phe Ser Leu Val Glu Glu Asp Gly Pro Pro Met Arg Glu 
                85                  90                  95      


Ile Ile Leu Lys Val Val Leu Met Ala Ile Cys Gly Met Asn Pro Ile 
            100                 105                 110         


Ala Ile Pro Phe Ala Ala Gly Ala Trp Tyr Val Tyr Val Lys Thr Gly 
        115                 120                 125             


Lys Arg 
    130 


<210>  46
<211>  617
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS3

<400>  46

Ser Gly Ala Leu Trp Asp Val Pro Ala Pro Lys Glu Val Lys Lys Gly 
1               5                   10                  15      


Glu Thr Thr Asp Gly Val Tyr Arg Val Met Thr Arg Arg Leu Leu Gly 
            20                  25                  30          


Ser Thr Gln Val Gly Val Gly Val Met Gln Glu Gly Val Phe His Thr 
        35                  40                  45              


Met Trp His Val Thr Lys Gly Ala Ala Leu Arg Ser Gly Glu Gly Arg 
    50                  55                  60                  


Leu Asp Pro Tyr Trp Gly Asp Val Lys Gln Asp Leu Val Ser Tyr Cys 
65                  70                  75                  80  


Gly Pro Trp Lys Leu Asp Ala Ala Trp Asp Gly Leu Ser Glu Val Gln 
                85                  90                  95      


Leu Leu Ala Val Pro Pro Gly Glu Arg Ala Arg Asn Ile Gln Thr Leu 
            100                 105                 110         


Pro Gly Ile Phe Lys Thr Lys Asp Gly Asp Ile Gly Ala Val Ala Leu 
        115                 120                 125             


Asp Tyr Pro Ala Gly Thr Ser Gly Ser Pro Ile Leu Asp Lys Cys Gly 
    130                 135                 140                 


Arg Val Ile Gly Leu Tyr Gly Asn Gly Val Val Ile Lys Asn Gly Ser 
145                 150                 155                 160 


Tyr Val Ser Ala Ile Thr Gln Gly Lys Arg Glu Glu Glu Thr Pro Val 
                165                 170                 175     


Glu Cys Phe Glu Pro Ser Met Leu Lys Lys Lys Gln Leu Thr Val Leu 
            180                 185                 190         


Asp Leu His Pro Gly Ala Gly Lys Thr Arg Arg Val Leu Pro Glu Ile 
        195                 200                 205             


Val Arg Glu Ala Ile Lys Lys Arg Leu Arg Thr Val Ile Leu Ala Pro 
    210                 215                 220                 


Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro 
225                 230                 235                 240 


Val Arg Tyr Met Thr Thr Ala Val Asn Val Thr His Ser Gly Thr Glu 
                245                 250                 255     


Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Ser Arg Leu Leu Gln 
            260                 265                 270         


Pro Ile Arg Val Pro Asn Tyr Asn Leu Asn Ile Met Asp Glu Ala His 
        275                 280                 285             


Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg Gly Tyr Ile Ser Thr Arg 
    290                 295                 300                 


Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met Thr Ala Thr Pro Pro 
305                 310                 315                 320 


Gly Thr Arg Asp Ala Phe Pro Asp Ser Asn Ser Pro Ile Met Asp Thr 
                325                 330                 335     


Glu Val Glu Val Pro Glu Arg Ala Trp Ser Ser Gly Phe Asp Trp Val 
            340                 345                 350         


Thr Asp His Ser Gly Lys Thr Val Trp Phe Val Pro Ser Val Arg Asn 
        355                 360                 365             


Gly Asn Glu Ile Ala Ala Cys Leu Thr Lys Ala Gly Lys Arg Val Ile 
    370                 375                 380                 


Gln Leu Ser Arg Lys Thr Phe Glu Thr Glu Phe Gln Lys Thr Lys Asn 
385                 390                 395                 400 


Gln Glu Trp Asp Phe Val Ile Thr Thr Asp Ile Ser Glu Met Gly Ala 
                405                 410                 415     


Asn Phe Lys Ala Asp Arg Val Ile Asp Ser Arg Arg Cys Leu Lys Pro 
            420                 425                 430         


Val Ile Leu Asp Gly Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val 
        435                 440                 445             


Thr His Ala Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro 
    450                 455                 460                 


Asn Lys Pro Gly Asp Glu Tyr Met Tyr Gly Gly Gly Cys Ala Glu Thr 
465                 470                 475                 480 


Asp Glu Gly His Ala His Trp Leu Glu Ala Arg Met Leu Leu Asp Asn 
                485                 490                 495     


Ile Tyr Leu Gln Asp Gly Leu Ile Ala Ser Leu Tyr Arg Pro Glu Ala 
            500                 505                 510         


Asp Lys Val Ala Ala Ile Glu Gly Glu Phe Lys Leu Arg Thr Glu Gln 
        515                 520                 525             


Arg Lys Thr Phe Val Glu Leu Met Lys Arg Gly Asp Leu Pro Val Trp 
    530                 535                 540                 


Leu Ala Tyr Gln Val Ala Ser Ala Gly Ile Thr Tyr Thr Asp Arg Arg 
545                 550                 555                 560 


Trp Cys Phe Asp Gly Thr Thr Asn Asn Thr Ile Met Glu Asp Ser Val 
                565                 570                 575     


Pro Ala Glu Val Trp Thr Lys Tyr Gly Glu Lys Arg Val Leu Lys Pro 
            580                 585                 590         


Arg Trp Met Asp Ala Arg Val Cys Ser Asp His Ala Ala Leu Lys Ser 
        595                 600                 605             


Phe Lys Glu Phe Ala Ala Gly Lys Arg 
    610                 615         


<210>  47
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS4A

<400>  47

Gly Ala Ala Leu Gly Val Met Glu Ala Leu Gly Thr Leu Pro Gly His 
1               5                   10                  15      


Met Thr Glu Arg Phe Gln Glu Ala Ile Asp Asn Leu Ala Val Leu Met 
            20                  25                  30          


Arg Ala Glu Thr Gly Ser Arg Pro Tyr Lys Ala Ala Ala Ala Gln Leu 
        35                  40                  45              


Pro Glu Thr Leu Glu Thr Ile Met Leu Leu Gly Leu Leu Gly Thr Val 
    50                  55                  60                  


Ser Leu Gly Ile Phe Phe Val Leu Met Arg Asn Lys Gly Ile Gly Lys 
65                  70                  75                  80  


Met Gly Phe Gly Met Val Thr Leu Gly Ala Ser Ala Trp Leu Met Trp 
                85                  90                  95      


Leu Ser Glu Ile Glu Pro Ala Arg Ile Ala Cys Val Leu Ile Val Val 
            100                 105                 110         


Phe Leu Leu Leu Val Val Leu Ile Pro Glu Pro Glu Lys Gln Arg Ser 
        115                 120                 125             


Pro Gln Asp Asn Gln Met Ala Ile Ile Ile Met Val Ala Val Gly Leu 
    130                 135                 140                 


Leu Gly Leu Ile Thr Ala 
145                 150 


<210>  48
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS4B

<400>  48

Asn Glu Leu Gly Trp Leu Glu Arg Thr Lys Asn Asp Ile Ala His Leu 
1               5                   10                  15      


Met Gly Arg Arg Glu Glu Gly Ala Thr Met Gly Phe Ser Met Asp Ile 
            20                  25                  30          


Asp Leu Arg Pro Ala Ser Ala Trp Ala Ile Tyr Ala Ala Leu Thr Thr 
        35                  40                  45              


Leu Ile Thr Pro Ala Val Gln His Ala Val Thr Thr Ser Tyr Asn Asn 
    50                  55                  60                  


Tyr Ser Leu Met Ala Met Ala Thr Gln Ala Gly Val Leu Phe Gly Met 
65                  70                  75                  80  


Gly Lys Gly Met Pro Phe Met His Gly Asp Leu Gly Val Pro Leu Leu 
                85                  90                  95      


Met Met Gly Cys Tyr Ser Gln Leu Thr Pro Leu Thr Leu Ile Val Ala 
            100                 105                 110         


Ile Ile Leu Leu Val Ala His Tyr Met Tyr Leu Ile Pro Gly Leu Gln 
        115                 120                 125             


Ala Ala Ala Ala Arg Ala Ala Gln Lys Arg Thr Ala Ala Gly Ile Met 
    130                 135                 140                 


Lys Asn Pro Val Val Asp Gly Ile Val Val Thr Asp Ile Asp Thr Met 
145                 150                 155                 160 


Thr Ile Asp Pro Gln Val Glu Lys Lys Met Gly Gln Val Leu Leu Ile 
                165                 170                 175     


Ala Val Ala Ile Ser Ser Ala Val Leu Leu Arg Thr Ala Trp Gly Trp 
            180                 185                 190         


Gly Glu Ala Gly Ala Leu Ile Thr Ala Ala Thr Ser Thr Leu Trp Glu 
        195                 200                 205             


Gly Ser Pro Asn Lys Tyr Trp Asn Ser Ser Thr Ala Thr Ser Leu Cys 
    210                 215                 220                 


Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Ser Leu Ile Tyr Thr 
225                 230                 235                 240 


Val Thr Arg Asn Ala Gly Leu Val Lys Arg Arg 
                245                 250     


<210>  49
<211>  903
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS5

<400>  49

Gly Gly Gly Thr Gly Glu Thr Leu Gly Glu Lys Trp Lys Ala Arg Leu 
1               5                   10                  15      


Asn Gln Met Ser Ala Leu Glu Phe Tyr Ser Tyr Lys Lys Ser Gly Ile 
            20                  25                  30          


Thr Glu Val Cys Arg Glu Glu Ala Arg Arg Ala Leu Lys Asp Gly Val 
        35                  40                  45              


Ala Thr Gly Gly His Ala Val Ser Arg Gly Ser Ala Lys Ile Arg Trp 
    50                  55                  60                  


Leu Glu Glu Arg Gly Tyr Leu Gln Pro Tyr Gly Lys Val Val Asp Leu 
65                  70                  75                  80  


Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Ala Ala Thr Ile Arg Lys 
                85                  90                  95      


Val Gln Glu Val Arg Gly Tyr Thr Lys Gly Gly Pro Gly His Glu Glu 
            100                 105                 110         


Pro Met Leu Val Gln Ser Tyr Gly Trp Asn Ile Val Arg Leu Lys Ser 
        115                 120                 125             


Gly Val Asp Val Phe His Met Ala Ala Glu Pro Cys Asp Thr Leu Leu 
    130                 135                 140                 


Cys Asp Ile Gly Glu Ser Ser Ser Ser Pro Glu Val Glu Glu Thr Arg 
145                 150                 155                 160 


Thr Leu Arg Val Leu Ser Met Val Gly Asp Trp Leu Glu Lys Arg Pro 
                165                 170                 175     


Gly Ala Phe Cys Ile Lys Val Leu Cys Pro Tyr Thr Ser Thr Met Met 
            180                 185                 190         


Glu Thr Met Glu Arg Leu Gln Arg Arg His Gly Gly Gly Leu Val Arg 
        195                 200                 205             


Val Pro Leu Cys Arg Asn Ser Thr His Glu Met Tyr Trp Val Ser Gly 
    210                 215                 220                 


Ala Lys Ser Asn Ile Ile Lys Ser Val Ser Thr Thr Ser Gln Leu Leu 
225                 230                 235                 240 


Leu Gly Arg Met Asp Gly Pro Arg Arg Pro Val Lys Tyr Glu Glu Asp 
                245                 250                 255     


Val Asn Leu Gly Ser Gly Thr Arg Ala Val Ala Ser Cys Ala Glu Ala 
            260                 265                 270         


Pro Asn Met Lys Ile Ile Gly Arg Arg Ile Glu Arg Ile Arg Asn Glu 
        275                 280                 285             


His Ala Glu Thr Trp Phe Leu Asp Glu Asn His Pro Tyr Arg Thr Trp 
    290                 295                 300                 


Ala Tyr His Gly Ser Tyr Glu Ala Pro Thr Gln Gly Ser Ala Ser Ser 
305                 310                 315                 320 


Leu Val Asn Gly Val Val Arg Leu Leu Ser Lys Pro Trp Asp Val Val 
                325                 330                 335     


Thr Gly Val Thr Gly Ile Ala Met Thr Asp Thr Thr Pro Tyr Gly Gln 
            340                 345                 350         


Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Val Pro Asp Pro Gln 
        355                 360                 365             


Glu Gly Thr Arg Gln Val Met Asn Ile Val Ser Ser Trp Leu Trp Lys 
    370                 375                 380                 


Glu Leu Gly Lys Arg Lys Arg Pro Arg Val Cys Thr Lys Glu Glu Phe 
385                 390                 395                 400 


Ile Asn Lys Val Arg Ser Asn Ala Ala Leu Gly Ala Ile Phe Glu Glu 
                405                 410                 415     


Glu Lys Glu Trp Lys Thr Ala Val Glu Ala Val Asn Asp Pro Arg Phe 
            420                 425                 430         


Trp Ala Leu Val Asp Arg Glu Arg Glu His His Leu Arg Gly Glu Cys 
        435                 440                 445             


His Ser Cys Val Tyr Asn Met Met Gly Lys Arg Glu Lys Lys Gln Gly 
    450                 455                 460                 


Glu Phe Gly Lys Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu 
465                 470                 475                 480 


Gly Ala Arg Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp 
                485                 490                 495     


His Trp Met Gly Arg Glu Asn Ser Gly Gly Gly Val Glu Gly Leu Gly 
            500                 505                 510         


Leu Gln Arg Leu Gly Tyr Ile Leu Glu Glu Met Asn Arg Ala Pro Gly 
        515                 520                 525             


Gly Lys Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Ser 
    530                 535                 540                 


Lys Phe Asp Leu Glu Asn Glu Ala Leu Ile Thr Asn Gln Met Glu Glu 
545                 550                 555                 560 


Gly His Arg Thr Leu Ala Leu Ala Val Ile Lys Tyr Thr Tyr Gln Asn 
                565                 570                 575     


Lys Val Val Lys Val Leu Arg Pro Ala Glu Gly Gly Lys Thr Val Met 
            580                 585                 590         


Asp Ile Ile Ser Arg Gln Asp Gln Arg Gly Ser Gly Gln Val Val Thr 
        595                 600                 605             


Tyr Ala Leu Asn Thr Phe Thr Asn Leu Val Val Gln Leu Ile Arg Asn 
    610                 615                 620                 


Met Glu Ala Glu Glu Val Leu Glu Met Gln Asp Leu Trp Leu Leu Arg 
625                 630                 635                 640 


Lys Pro Glu Lys Val Thr Arg Trp Leu Gln Ser Asn Gly Trp Asp Arg 
                645                 650                 655     


Leu Lys Arg Met Ala Val Ser Gly Asp Asp Cys Val Val Lys Pro Ile 
            660                 665                 670         


Asp Asp Arg Phe Ala His Ala Leu Arg Phe Leu Asn Asp Met Gly Lys 
        675                 680                 685             


Val Arg Lys Asp Thr Gln Glu Trp Lys Pro Ser Thr Gly Trp Ser Asn 
    690                 695                 700                 


Trp Glu Glu Val Pro Phe Cys Ser His His Phe Asn Lys Leu Tyr Leu 
705                 710                 715                 720 


Lys Asp Gly Arg Ser Ile Val Val Pro Cys Arg His Gln Asp Glu Leu 
                725                 730                 735     


Ile Gly Arg Ala Arg Val Ser Pro Gly Ala Gly Trp Ser Ile Arg Glu 
            740                 745                 750         


Thr Ala Cys Leu Ala Lys Ser Tyr Ala Gln Met Trp Gln Leu Leu Tyr 
        755                 760                 765             


Phe His Arg Arg Asp Leu Arg Leu Met Ala Asn Ala Ile Cys Ser Ala 
    770                 775                 780                 


Val Pro Val Asp Trp Val Pro Thr Gly Arg Thr Thr Trp Ser Ile His 
785                 790                 795                 800 


Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Met Val Trp Asn 
                805                 810                 815     


Arg Val Trp Ile Glu Glu Asn Asp His Met Glu Asp Lys Thr Pro Val 
            820                 825                 830         


Thr Lys Trp Thr Asp Ile Pro Tyr Leu Gly Lys Arg Glu Asp Leu Trp 
        835                 840                 845             


Cys Gly Ser Leu Ile Gly His Arg Pro Arg Thr Thr Trp Ala Glu Asn 
    850                 855                 860                 


Ile Lys Asp Thr Val Asn Met Val Arg Arg Ile Ile Gly Asp Glu Glu 
865                 870                 875                 880 


Lys Tyr Met Asp Tyr Leu Ser Thr Gln Val Arg Tyr Leu Gly Glu Glu 
                885                 890                 895     


Gly Ser Thr Pro Gly Val Leu 
            900             


<210>  50
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800)

<400>  50

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr 
            340                 345                 350         


Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
        355                 360                 365             


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
    370                 375                 380                 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
385                 390                 395                 400 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
                405                 410                 415     


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
            420                 425                 430         


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
        435                 440                 445             


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
    450                 455                 460                 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser 
465                 470                 475                 480 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
                485                 490                 495     


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
            500                 505                 510         


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
        515                 520                 525             


Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln 
    530                 535                 540                 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
545                 550                 555                 560 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
                565                 570                 575     


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
            580                 585                 590         


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
        595                 600                 605             


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
    610                 615                 620                 


Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His 
625                 630                 635                 640 


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
                645                 650                 655     


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn 
            660                 665                 670         


Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val 
        675                 680                 685             


Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
    690                 695                 700                 


Asp Phe Ser Lys 
705             


<210>  51
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719)

<400>  51

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu 
                165                 170                 175     


Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala 
            180                 185                 190         


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
        195                 200                 205             


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
    210                 215                 220                 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
225                 230                 235                 240 


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
                245                 250                 255     


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
            260                 265                 270         


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
        275                 280                 285             


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys 
    290                 295                 300                 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
305                 310                 315                 320 


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu 
                325                 330                 335     


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
            340                 345                 350         


Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
        355                 360                 365             


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
    370                 375                 380                 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
385                 390                 395                 400 


Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg 
                405                 410                 415     


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
            420                 425                 430         


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
        435                 440                 445         


<210>  52
<211>  10269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein CDS mRNA wild type

<400>  52
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcaauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac ggggcgcaga uacuaguguc ggaauuguug gccuccugcu gaccacagcu      360

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      420

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      480

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      540

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      600

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      660

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      720

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      780

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      900

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      960

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc     1020

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg     1080

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa     1200

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc     1260

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1320

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1380

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1440

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1500

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1560

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1620

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1680

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1740

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1800

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1860

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1920

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1980

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     2040

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     2100

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     2160

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     2220

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     2280

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2340

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     2400

gacuucucaa agaaggagac gagaugcggu acaggggugu ucgucuauaa cgacguugaa     2460

gccuggaggg acagguacaa guaccauccu gacucccccc guagauuggc agcagcaguc     2520

aagcaagccu gggaagaugg uaucugcggg aucuccucug uuucaagaau ggaaaacauc     2580

auguggagau caguagaagg ggagcucaac gcaauccugg aagagaaugg aguucaacug     2640

acggucguug ugggaucugu aaaaaacccc auguggagag guccacagag auugcccgug     2700

ccugugaacg agcugcccca cggcuggaag gcuuggggga aaucgcacuu cgucagagca     2760

gcaaagacaa auaacagcuu ugucguggau ggugacacac ugaaggaaug cccacucaaa     2820

cauagagcau ggaacagcuu ucuuguggag gaucaugggu ucgggguauu ucacacuagu     2880

gucuggcuca agguuagaga agauuauuca uuagagugug auccagccgu uauuggaaca     2940

gcuguuaagg gaaaggaggc uguacacagu gaucuaggcu acuggauuga gagugagaag     3000

aaugacacau ggaggcugaa gagggcccau cugaucgaga ugaaaacaug ugaauggcca     3060

aagucccaca cauuguggac agauggaaua gaagagagug aucugaucau acccaagucu     3120

uuagcugggc cacucagcca ucacaauacc agagagggcu acaggaccca aaugaaaggg     3180

ccauggcaca gugaagagcu ugaaauucgg uuugaggaau gcccaggcac uaagguccac     3240

guggaggaaa cauguggaac aagaggacca ucucugagau caaccacugc aagcggaagg     3300

gugaucgagg aauggugcug cagggagugc acaaugcccc cacugucguu ccgggcuaaa     3360

gauggcuguu gguauggaau ggagauaagg cccaggaaag aaccagaaag caacuuagua     3420

aggucaaugg ugacugcagg aucaacugau cacauggauc acuucucccu uggagugcuu     3480

gugauucugc ucauggugca ggaagggcug aagaagagaa ugaccacaaa gaucaucaua     3540

agcacaucaa uggcagugcu gguagcuaug auccugggag gauuuucaau gagugaccug     3600

gcuaagcuug caauuuugau gggugccacc uucgcggaaa ugaacacugg aggagaugua     3660

gcucaucugg cgcugauagc ggcauucaaa gucagaccag cguugcuggu aucuuucauc     3720

uucagagcua auuggacacc ccgugaaagc augcugcugg ccuuggccuc gugucuuuug     3780

caaacugcga ucuccgccuu ggaaggcgac cugaugguuc ucaucaaugg uuuugcuuug     3840

gccugguugg caauacgagc gaugguuguu ccacgcacug auaacaucac cuuggcaauc     3900

cuggcugcuc ugacaccacu ggcccggggc acacugcuug uggcguggag agcaggccuu     3960

gcuacuugcg ggggguuuau gcuccucucu cugaagggaa aaggcagugu gaagaagaac     4020

uuaccauuug ucauggcccu gggacuaacc gcugugaggc uggucgaccc caucaacgug     4080

guggggcugc uguugcucac aaggaguggg aagcggagcu ggcccccuag cgaaguacuc     4140

acagcuguug gccugauaug cgcauuggcu ggaggguucg ccaaggcaga uauagagaug     4200

gcugggccca uggccgcggu cggucugcua auugucaguu acguggucuc aggaaagagu     4260

guggacaugu acauugaaag agcaggugac aucacauggg aaaaagaugc ggaagucacu     4320

ggaaacaguc cccggcucga uguggcgcua gaugagagug gugauuucuc ccugguggag     4380

gaugacgguc cccccaugag agagaucaua cucaaggugg uccugaugac caucuguggc     4440

augaacccaa uagccauacc cuuugcagcu ggagcguggu acguauacgu gaagacugga     4500

aaaaggagug gugcucuaug ggaugugccu gcucccaagg aaguaaaaaa gggggagacc     4560

acagauggag uguacagagu aaugacucgu agacugcuag guucaacaca aguuggagug     4620

ggaguuaugc aagagggggu cuuucacacu auguggcacg ucacaaaagg auccgcgcug     4680

agaagcggug aagggagacu ugauccauac uggggagaug ucaagcagga ucugguguca     4740

uacugugguc cauggaagcu agaugccgcc ugggacgggc acagcgaggu gcagcucuug     4800

gccgugcccc ccggagagag agcgaggaac auccagacuc ugcccggaau auuuaagaca     4860

aaggaugggg acauuggagc gguugcgcug gauuacccag caggaacuuc aggaucucca     4920

auccuagaca agugugggag agugauagga cuuuauggca auggggucgu gaucaaaaau     4980

gggaguuaug uuagugccau cacccaaggg aggagggagg aagagacucc uguugagugc     5040

uucgagccuu cgaugcugaa gaagaagcag cuaacugucu uagacuugca uccuggagcu     5100

gggaaaacca ggagaguucu uccugaaaua guccgugaag ccauaaaaac aagacuccgu     5160

acugugaucu uagcuccaac caggguuguc gcugcugaaa uggaggaagc ccuuagaggg     5220

cuuccagugc guuauaugac aacagcaguc aaugucaccc acucuggaac agaaaucguc     5280

gacuuaaugu gccaugccac cuucacuuca cgucuacuac agccaaucag aguccccaac     5340

uauaaucugu auauuaugga ugaggcccac uucacagauc ccucaaguau agcagcaaga     5400

ggauacauuu caacaagggu ugagaugggc gaggcggcug ccaucuucau gaccgccacg     5460

ccaccaggaa cccgugacgc auuuccggac uccaacucac caauuaugga caccgaagug     5520

gaagucccag agagagccug gagcucaggc uuugauuggg ugacggauua uucuggaaaa     5580

acaguuuggu uuguuccaag cgugaggaac ggcaaugaga ucgcagcuug ucugacaaag     5640

gcuggaaaac gggucauaca gcucagcaga aagacuuuug agacagaguu ccagaaaaca     5700

aaacaucaag agugggacuu ugucgugaca acugacauuu cagagauggg cgccaacuuu     5760

aaagcugacc gugucauaga uuccaggaga ugccuaaagc cggucauacu ugauggcgag     5820

agagucauuc uggcuggacc caugccuguc acacaugcca gcgcugccca gaggaggggg     5880

cgcauaggca ggaaucccaa caaaccugga gaugaguauc uguauggagg ugggugcgca     5940

gagacugacg aagaccaugc acacuggcuu gaagcaagaa ugcuccuuga caauauuuac     6000

cuccaagaug gccucauagc cucgcucuau cgaccugagg ccgacaaagu agcagccauu     6060

gagggagagu ucaagcuuag gacggagcaa aggaagaccu uuguggaacu caugaaaaga     6120

ggagaucuuc cuguuuggcu ggccuaucag guugcaucug ccggaauaac cuacacagau     6180

agaagauggu gcuuugaugg cacgaccaac aacaccauaa uggaagacag ugugccggca     6240

gaggugugga ccagacacgg agagaaaaga gugcucaaac cgagguggau ggacgccaga     6300

guuuguucag aucaugcggc ccugaaguca uucaaggagu uugccgcugg gaaaagagga     6360

gcggcuuuug gagugaugga agcccuggga acacugccag gacacaugac agagagauuc     6420

caggaagcca uugacaaccu cgcugugcuc augcgggcag agacuggaag caggccuuac     6480

aaagccgcgg cggcccaauu gccggagacc cuagagacca uuaugcuuuu gggguugcug     6540

ggaacagucu cgcugggaau cuuuuucguc uugaugagga acaagggcau agggaagaug     6600

ggcuuuggaa uggugacucu uggggccagc gcauggcuca uguggcucuc ggaaauugag     6660

ccagccagaa uugcaugugu ccucauuguu guguuccuau ugcugguggu gcucauaccu     6720

gagccagaaa agcaaagauc uccccaggac aaccaaaugg caaucaucau caugguagca     6780

guaggucuuc ugggcuugau uaccgccaau gaacucggau gguuggagag aacaaagagu     6840

gaccuaagcc aucuaauggg aaggagagag gagggggcaa ccaugggauu cucaauggac     6900

auugaccugc ggccagccuc agcuugggcc aucuaugcug ccuugacaac uuucauuacc     6960

ccagccgucc aacaugcagu gaccacuuca uacaacaacu acuccuuaau ggcgauggcc     7020

acgcaagcug gaguguuguu ugguaugggc aaagggaugc cauucuacgc augggacuuu     7080

ggagucccgc ugcuaaugau agguugcuac ucacaauuaa cgccccugac ccuaauagug     7140

gccaucauuu ugcucguggc gcacuacaug uacuugaucc cagggcugca ggcagcagcu     7200

gcgcgugcug cccagaagag aacggcagcu ggcaucauga agaacccugu uguggaugga     7260

auagugguga cugacauuga cacaaugaca auugaccccc aaguggagaa aaagauggga     7320

caggugcuac ucauggcagu agccgucucc agcgccauac ugucgcggac cgccuggggg     7380

uggggggagg cuggggcccu gaucacagcc gcaacuucca cuuuguggga aggcucuccg     7440

aacaaguacu ggaacuccuc uacagccacu ucacugugua acauuuuuag gggaaguuac     7500

uuggcuggag cuucucuaau cuacacagua acaagaaacg cuggcuuggu caagagacgu     7560

ggggguggaa caggagagac ccugggagag aaauggaagg cccgcuugaa ccagaugucg     7620

gcccuggagu ucuacuccua caaaaaguca ggcaucaccg aggugugcag agaagaggcc     7680

cgccgcgccc ucaaggacgg uguggcaacg ggaggccaug cugugucccg aggaagugca     7740

aagcugagau gguuggugga gcggggauac cugcagcccu auggaaaggu cauugaucuu     7800

ggauguggca gagggggcug gaguuacuac gccgccacca uccgcaaagu ucaagaagug     7860

aaaggauaca caaaaggagg cccuggucau gaagaacccg uguuggugca aagcuauggg     7920

uggaacauag uccgucuuaa gaguggggug gacgucuuuc auauggcggc ugagccgugu     7980

gacacguugc ugugugacau aggugaguca ucaucuaguc cugaagugga agaagcacgg     8040

acgcucagag uccucuccau ggugggggau uggcuugaaa aaagaccagg agccuuuugu     8100

auaaaagugu ugugcccaua caccagcacu augauggaaa cccuggagcg acugcagcgu     8160

agguaugggg gaggacuggu cagagugcca cucucccgca acucuacaca ugagauguac     8220

ugggucucug gagcgaaaag caacaccaua aaaagugugu ccaccacgag ccagcuccuc     8280

uuggggcgca uggacgggcc uaggaggcca gugaaauaug aggaggaugu gaaucucggc     8340

ucuggcacgc gggcuguggu aagcugcgcu gaagcuccca acaugaagau cauugguaac     8400

cgcauugaaa ggauccgcag ugagcacgcg gaaacguggu ucuuugacga gaaccaccca     8460

uauaggacau gggcuuacca uggaagcuau gaggccccca cacaaggguc agcguccucu     8520

cuaauaaacg ggguugucag gcuccuguca aaacccuggg auguggugac uggagucaca     8580

ggaauagcca ugaccgacac cacaccguau ggucagcaaa gaguuuucaa ggaaaaagug     8640

gacacuaggg ugccagaccc ccaagaaggu acucgucagg uuaugagcau ggucucuucc     8700

ugguugugga aagagcuagg caaacacaaa cggccacgag ucuguaccaa agaagaguuc     8760

aucaacaagg uucguagcaa ugcagcauua ggggcaauau uugaagagga aaaagagugg     8820

aagacugcag uggaagcugu gaacgaucca agguucuggg cucuagugga caaggaaaga     8880

gagcaccacc ugagaggaga gugccagagu uguguguaca acaugauggg aaaaagagaa     8940

aagaaacaag gggaauuugg aaaggccaag ggcagccgcg ccaucuggua uauguggcua     9000

ggggcuagau uucuagaguu cgaagcccuu ggauucuuga acgaggauca cuggaugggg     9060

agagagaacu caggaggugg uguugaaggg cugggauuac aaagacucgg auauguccua     9120

gaagagauga gucgcauacc aggaggaagg auguaugcag augacacugc uggcugggac     9180

acccgcauca gcagguuuga ucuggagaau gaagcucuaa ucaccaacca aauggagaaa     9240

gggcacaggg ccuuggcauu ggccauaauc aaguacacau accaaaacaa agugguaaag     9300

guccuuagac cagcugaaaa agggaaaaca guuauggaca uuauuucgag acaagaccaa     9360

agggggagcg gacaaguugu cacuuacgcu cuuaacacau uuaccaaccu aguggugcaa     9420

cucauucgga auauggaggc ugaggaaguc cuagagaugc aagacuugug gcugcugcgg     9480

aggucagaga aagugaccaa cugguugcag agcaacggau gggauaggcu caaacgaaug     9540

gcagucagug gagaugauug cguugugaag ccaauugaug auagguuugc acaugcccuc     9600

agguucuuga augauauggg aaaaguuagg aaggacacac aagaguggaa acccucaacu     9660

ggaugggaca acugggaaga aguuccguuu ugcucccacc acuucaacaa gcuccaucuc     9720

aaggacggga gguccauugu gguucccugc cgccaccaag augaacugau uggccgggcc     9780

cgcgucucuc caggggcggg auggagcauc cgggagacug cuugccuagc aaaaucauau     9840

gcgcaaaugu ggcagcuccu uuauuuccac agaagggacc uccgacugau ggccaaugcc     9900

auuuguucau cugugccagu ugacuggguu ccaacuggga gaacuaccug gucaauccau     9960

ggaaagggag aauggaugac cacugaagac augcuugugg uguggaacag aguguggauu    10020

gaggagaacg accacaugga agacaagacc ccaguuacga aauggacaga cauucccuau    10080

uugggaaaaa gggaagacuu guggugugga ucucucauag ggcacagacc gcgcaccacc    10140

ugggcugaga acauuaaaaa cacagucaac auggugcgca ggaucauagg ugaugaagaa    10200

aaguacaugg acuaccuauc cacccaaguu cgcuacuugg gugaagaagg gucuacaccu    10260

ggagugcug                                                            10269


<210>  53
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C CDS mRNA wild type

<400>  53
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcaauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac gg                                                          312


<210>  54
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-ER anchor CDS mRNA wild type

<400>  54
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcaauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac ggggcgcaga uacuaguguc ggaauuguug gccuccugcu gaccacagcu      360

auggca                                                                 366


<210>  55
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) pr CDS mRNA wild type

<400>  55
gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg       60

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      120

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      180

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      240

ugccaucaca aaaaagguga agcacggaga ucuagaaga                             279


<210>  56
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) M CDS mRNA wild type

<400>  56
gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug       60

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      120

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      180

gucauauacu uggucaugau acugcugauu gccccggcau acagc                      225


<210>  57
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prM CDS mRNA wild type

<400>  57
gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg       60

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      120

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      180

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      240

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      300

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      360

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      420

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      480

cugcugauug ccccggcaua cagc                                             504


<210>  58
<211>  1512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) E CDS mRNA wild type

<400>  58
aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg       60

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      120

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      180

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      240

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      300

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      360

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug      420

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu      480

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug      540

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau      600

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac      660

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa      720

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu      780

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca      840

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug      900

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa      960

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1020

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1080

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1140

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1200

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1260

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1320

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1380

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     1440

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     1500

gccgucucug cu                                                         1512


<210>  59
<211>  2016
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME CDS mRNA wild type

<400>  59
gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg       60

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      120

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      180

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      240

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      300

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      360

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      420

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      480

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      540

gaagguaugu caggugggac uuggguugau auugucuugg aacauggagg uugugucacc      600

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      660

auggcggagg uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc      720

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      780

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu      840

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      900

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc      960

guuaaugaca caggacauga aacugaugag aauagagcga agguugagau aacgcccaau     1020

ucaccaagag ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg     1080

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug     1140

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1200

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1260

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1320

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1380

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc     1440

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1500

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1560

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1620

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1680

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1740

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1800

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu     1860

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1920

augugguugg gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     1980

ggaguguuga ucuucuuauc cacagccguc ucugcu                               2016


<210>  60
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS1 CDS mRNA wild type

<400>  60
gauguggggu gcucggugga cuucucaaag aaggagacga gaugcgguac agggguguuc       60

gucuauaacg acguugaagc cuggagggac agguacaagu accauccuga cuccccccgu      120

agauuggcag cagcagucaa gcaagccugg gaagauggua ucugcgggau cuccucuguu      180

ucaagaaugg aaaacaucau guggagauca guagaagggg agcucaacgc aauccuggaa      240

gagaauggag uucaacugac ggucguugug ggaucuguaa aaaaccccau guggagaggu      300

ccacagagau ugcccgugcc ugugaacgag cugccccacg gcuggaaggc uugggggaaa      360

ucgcacuucg ucagagcagc aaagacaaau aacagcuuug ucguggaugg ugacacacug      420

aaggaaugcc cacucaaaca uagagcaugg aacagcuuuc uuguggagga ucauggguuc      480

gggguauuuc acacuagugu cuggcucaag guuagagaag auuauucauu agagugugau      540

ccagccguua uuggaacagc uguuaaggga aaggaggcug uacacaguga ucuaggcuac      600

uggauugaga gugagaagaa ugacacaugg aggcugaaga gggcccaucu gaucgagaug      660

aaaacaugug aauggccaaa gucccacaca uuguggacag auggaauaga agagagugau      720

cugaucauac ccaagucuuu agcugggcca cucagccauc acaauaccag agagggcuac      780

aggacccaaa ugaaagggcc auggcacagu gaagagcuug aaauucgguu ugaggaaugc      840

ccaggcacua agguccacgu ggaggaaaca uguggaacaa gaggaccauc ucugagauca      900

accacugcaa gcggaagggu gaucgaggaa uggugcugca gggagugcac aaugccccca      960

cugucguucc gggcuaaaga uggcuguugg uauggaaugg agauaaggcc caggaaagaa     1020

ccagaaagca acuuaguaag gucaauggug acugca                               1056


<210>  61
<211>  678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS2A CDS mRNA wild type

<400>  61
ggaucaacug aucacaugga ucacuucucc cuuggagugc uugugauucu gcucauggug       60

caggaagggc ugaagaagag aaugaccaca aagaucauca uaagcacauc aauggcagug      120

cugguagcua ugauccuggg aggauuuuca augagugacc uggcuaagcu ugcaauuuug      180

augggugcca ccuucgcgga aaugaacacu ggaggagaug uagcucaucu ggcgcugaua      240

gcggcauuca aagucagacc agcguugcug guaucuuuca ucuucagagc uaauuggaca      300

ccccgugaaa gcaugcugcu ggccuuggcc ucgugucuuu ugcaaacugc gaucuccgcc      360

uuggaaggcg accugauggu ucucaucaau gguuuugcuu uggccugguu ggcaauacga      420

gcgaugguug uuccacgcac ugauaacauc accuuggcaa uccuggcugc ucugacacca      480

cuggcccggg gcacacugcu uguggcgugg agagcaggcc uugcuacuug cgggggguuu      540

augcuccucu cucugaaggg aaaaggcagu gugaagaaga acuuaccauu ugucauggcc      600

cugggacuaa ccgcugugag gcuggucgac cccaucaacg ugguggggcu gcuguugcuc      660

acaaggagug ggaagcgg                                                    678


<210>  62
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS2B CDS mRNA wild type

<400>  62
agcuggcccc cuagcgaagu acucacagcu guuggccuga uaugcgcauu ggcuggaggg       60

uucgccaagg cagauauaga gauggcuggg cccauggccg cggucggucu gcuaauuguc      120

aguuacgugg ucucaggaaa gaguguggac auguacauug aaagagcagg ugacaucaca      180

ugggaaaaag augcggaagu cacuggaaac aguccccggc ucgauguggc gcuagaugag      240

aguggugauu ucucccuggu ggaggaugac ggucccccca ugagagagau cauacucaag      300

gugguccuga ugaccaucug uggcaugaac ccaauagcca uacccuuugc agcuggagcg      360

ugguacguau acgugaagac uggaaaaagg                                       390


<210>  63
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS3 CDS mRNA wild type

<400>  63
aguggugcuc uaugggaugu gccugcuccc aaggaaguaa aaaaggggga gaccacagau       60

ggaguguaca gaguaaugac ucguagacug cuagguucaa cacaaguugg agugggaguu      120

augcaagagg gggucuuuca cacuaugugg cacgucacaa aaggauccgc gcugagaagc      180

ggugaaggga gacuugaucc auacugggga gaugucaagc aggaucuggu gucauacugu      240

gguccaugga agcuagaugc cgccugggac gggcacagcg aggugcagcu cuuggccgug      300

ccccccggag agagagcgag gaacauccag acucugcccg gaauauuuaa gacaaaggau      360

ggggacauug gagcgguugc gcuggauuac ccagcaggaa cuucaggauc uccaauccua      420

gacaagugug ggagagugau aggacuuuau ggcaaugggg ucgugaucaa aaaugggagu      480

uauguuagug ccaucaccca agggaggagg gaggaagaga cuccuguuga gugcuucgag      540

ccuucgaugc ugaagaagaa gcagcuaacu gucuuagacu ugcauccugg agcugggaaa      600

accaggagag uucuuccuga aauaguccgu gaagccauaa aaacaagacu ccguacugug      660

aucuuagcuc caaccagggu ugucgcugcu gaaauggagg aagcccuuag agggcuucca      720

gugcguuaua ugacaacagc agucaauguc acccacucug gaacagaaau cgucgacuua      780

augugccaug ccaccuucac uucacgucua cuacagccaa ucagaguccc caacuauaau      840

cuguauauua uggaugaggc ccacuucaca gaucccucaa guauagcagc aagaggauac      900

auuucaacaa ggguugagau gggcgaggcg gcugccaucu ucaugaccgc cacgccacca      960

ggaacccgug acgcauuucc ggacuccaac ucaccaauua uggacaccga aguggaaguc     1020

ccagagagag ccuggagcuc aggcuuugau ugggugacgg auuauucugg aaaaacaguu     1080

ugguuuguuc caagcgugag gaacggcaau gagaucgcag cuugucugac aaaggcugga     1140

aaacggguca uacagcucag cagaaagacu uuugagacag aguuccagaa aacaaaacau     1200

caagaguggg acuuugucgu gacaacugac auuucagaga ugggcgccaa cuuuaaagcu     1260

gaccguguca uagauuccag gagaugccua aagccgguca uacuugaugg cgagagaguc     1320

auucuggcug gacccaugcc ugucacacau gccagcgcug cccagaggag ggggcgcaua     1380

ggcaggaauc ccaacaaacc uggagaugag uaucuguaug gaggugggug cgcagagacu     1440

gacgaagacc augcacacug gcuugaagca agaaugcucc uugacaauau uuaccuccaa     1500

gauggccuca uagccucgcu cuaucgaccu gaggccgaca aaguagcagc cauugaggga     1560

gaguucaagc uuaggacgga gcaaaggaag accuuugugg aacucaugaa aagaggagau     1620

cuuccuguuu ggcuggccua ucagguugca ucugccggaa uaaccuacac agauagaaga     1680

uggugcuuug auggcacgac caacaacacc auaauggaag acagugugcc ggcagaggug     1740

uggaccagac acggagagaa aagagugcuc aaaccgaggu ggauggacgc cagaguuugu     1800

ucagaucaug cggcccugaa gucauucaag gaguuugccg cugggaaaag a              1851


<210>  64
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS4A CDS mRNA wild type

<400>  64
ggagcggcuu uuggagugau ggaagcccug ggaacacugc caggacacau gacagagaga       60

uuccaggaag ccauugacaa ccucgcugug cucaugcggg cagagacugg aagcaggccu      120

uacaaagccg cggcggccca auugccggag acccuagaga ccauuaugcu uuugggguug      180

cugggaacag ucucgcuggg aaucuuuuuc gucuugauga ggaacaaggg cauagggaag      240

augggcuuug gaauggugac ucuuggggcc agcgcauggc ucauguggcu cucggaaauu      300

gagccagcca gaauugcaug uguccucauu guuguguucc uauugcuggu ggugcucaua      360

ccugagccag aaaagcaaag aucuccccag gacaaccaaa uggcaaucau caucauggua      420

gcaguagguc uucugggcuu gauuaccgcc                                       450


<210>  65
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS4B CDS mRNA wild type

<400>  65
aaugaacucg gaugguugga gagaacaaag agugaccuaa gccaucuaau gggaaggaga       60

gaggaggggg caaccauggg auucucaaug gacauugacc ugcggccagc cucagcuugg      120

gccaucuaug cugccuugac aacuuucauu accccagccg uccaacaugc agugaccacu      180

ucauacaaca acuacuccuu aauggcgaug gccacgcaag cuggaguguu guuugguaug      240

ggcaaaggga ugccauucua cgcaugggac uuuggagucc cgcugcuaau gauagguugc      300

uacucacaau uaacgccccu gacccuaaua guggccauca uuuugcucgu ggcgcacuac      360

auguacuuga ucccagggcu gcaggcagca gcugcgcgug cugcccagaa gagaacggca      420

gcuggcauca ugaagaaccc uguuguggau ggaauagugg ugacugacau ugacacaaug      480

acaauugacc cccaagugga gaaaaagaug ggacaggugc uacucauggc aguagccguc      540

uccagcgcca uacugucgcg gaccgccugg gggugggggg aggcuggggc ccugaucaca      600

gccgcaacuu ccacuuugug ggaaggcucu ccgaacaagu acuggaacuc cucuacagcc      660

acuucacugu guaacauuuu uaggggaagu uacuuggcug gagcuucucu aaucuacaca      720

guaacaagaa acgcuggcuu ggucaagaga cgu                                   753


<210>  66
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS5 CDS mRNA wild type

<400>  66
ggggguggaa caggagagac ccugggagag aaauggaagg cccgcuugaa ccagaugucg       60

gcccuggagu ucuacuccua caaaaaguca ggcaucaccg aggugugcag agaagaggcc      120

cgccgcgccc ucaaggacgg uguggcaacg ggaggccaug cugugucccg aggaagugca      180

aagcugagau gguuggugga gcggggauac cugcagcccu auggaaaggu cauugaucuu      240

ggauguggca gagggggcug gaguuacuac gccgccacca uccgcaaagu ucaagaagug      300

aaaggauaca caaaaggagg cccuggucau gaagaacccg uguuggugca aagcuauggg      360

uggaacauag uccgucuuaa gaguggggug gacgucuuuc auauggcggc ugagccgugu      420

gacacguugc ugugugacau aggugaguca ucaucuaguc cugaagugga agaagcacgg      480

acgcucagag uccucuccau ggugggggau uggcuugaaa aaagaccagg agccuuuugu      540

auaaaagugu ugugcccaua caccagcacu augauggaaa cccuggagcg acugcagcgu      600

agguaugggg gaggacuggu cagagugcca cucucccgca acucuacaca ugagauguac      660

ugggucucug gagcgaaaag caacaccaua aaaagugugu ccaccacgag ccagcuccuc      720

uuggggcgca uggacgggcc uaggaggcca gugaaauaug aggaggaugu gaaucucggc      780

ucuggcacgc gggcuguggu aagcugcgcu gaagcuccca acaugaagau cauugguaac      840

cgcauugaaa ggauccgcag ugagcacgcg gaaacguggu ucuuugacga gaaccaccca      900

uauaggacau gggcuuacca uggaagcuau gaggccccca cacaaggguc agcguccucu      960

cuaauaaacg ggguugucag gcuccuguca aaacccuggg auguggugac uggagucaca     1020

ggaauagcca ugaccgacac cacaccguau ggucagcaaa gaguuuucaa ggaaaaagug     1080

gacacuaggg ugccagaccc ccaagaaggu acucgucagg uuaugagcau ggucucuucc     1140

ugguugugga aagagcuagg caaacacaaa cggccacgag ucuguaccaa agaagaguuc     1200

aucaacaagg uucguagcaa ugcagcauua ggggcaauau uugaagagga aaaagagugg     1260

aagacugcag uggaagcugu gaacgaucca agguucuggg cucuagugga caaggaaaga     1320

gagcaccacc ugagaggaga gugccagagu uguguguaca acaugauggg aaaaagagaa     1380

aagaaacaag gggaauuugg aaaggccaag ggcagccgcg ccaucuggua uauguggcua     1440

ggggcuagau uucuagaguu cgaagcccuu ggauucuuga acgaggauca cuggaugggg     1500

agagagaacu caggaggugg uguugaaggg cugggauuac aaagacucgg auauguccua     1560

gaagagauga gucgcauacc aggaggaagg auguaugcag augacacugc uggcugggac     1620

acccgcauca gcagguuuga ucuggagaau gaagcucuaa ucaccaacca aauggagaaa     1680

gggcacaggg ccuuggcauu ggccauaauc aaguacacau accaaaacaa agugguaaag     1740

guccuuagac cagcugaaaa agggaaaaca guuauggaca uuauuucgag acaagaccaa     1800

agggggagcg gacaaguugu cacuuacgcu cuuaacacau uuaccaaccu aguggugcaa     1860

cucauucgga auauggaggc ugaggaaguc cuagagaugc aagacuugug gcugcugcgg     1920

aggucagaga aagugaccaa cugguugcag agcaacggau gggauaggcu caaacgaaug     1980

gcagucagug gagaugauug cguugugaag ccaauugaug auagguuugc acaugcccuc     2040

agguucuuga augauauggg aaaaguuagg aaggacacac aagaguggaa acccucaacu     2100

ggaugggaca acugggaaga aguuccguuu ugcucccacc acuucaacaa gcuccaucuc     2160

aaggacggga gguccauugu gguucccugc cgccaccaag augaacugau uggccgggcc     2220

cgcgucucuc caggggcggg auggagcauc cgggagacug cuugccuagc aaaaucauau     2280

gcgcaaaugu ggcagcuccu uuauuuccac agaagggacc uccgacugau ggccaaugcc     2340

auuuguucau cugugccagu ugacuggguu ccaacuggga gaacuaccug gucaauccau     2400

ggaaagggag aauggaugac cacugaagac augcuugugg uguggaacag aguguggauu     2460

gaggagaacg accacaugga agacaagacc ccaguuacga aauggacaga cauucccuau     2520

uugggaaaaa gggaagacuu guggugugga ucucucauag ggcacagacc gcgcaccacc     2580

ugggcugaga acauuaaaaa cacagucaac auggugcgca ggaucauagg ugaugaagaa     2640

aaguacaugg acuaccuauc cacccaaguu cgcuacuugg gugaagaagg gucuacaccu     2700

ggagugcug                                                             2709


<210>  67
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS mRNA wild type

<400>  67
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  68
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS mRNA wild type

<400>  68
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  69
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS mRNA wild type

<400>  69
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  70
<211>  10269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein CDS mRNA wild type

<400>  70
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcgauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac gaggcgcaga uacuaguguc ggaauuguug gccuccugcu gaccacagcu      360

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      420

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      480

auggaucuug gacacacgug ugaugccacc augagcuaug aaugcccuau gcuggaugag      540

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      600

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      660

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      720

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      780

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      900

uuuguggaag guaugucagg ugggacuugg guugauguug ucuuggaaca uggagguugu      960

gucacuguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc     1020

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg     1080

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa     1200

gggagccugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc     1260

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1320

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaagu ugagauaacg     1380

cccaauucac caagagccga agccacccug gggggguuug gaagccuagg acuugauugu     1440

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1500

uggcugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1560

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1620

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1680

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1740

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguacugca     1800

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1860

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1920

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1980

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     2040

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     2100

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     2160

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aaucuuugga     2220

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     2280

uugcugaugu gguugggucu gaacgcaaag aauggaucua uuucccuuau gugcuuggcc     2340

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     2400

gacuucucaa agaaggagac gagaugcggu acaggggugu ucgucuauaa cgacguugaa     2460

gccuggaggg acagguacaa guaccauccu gacucccccc guagauuggc agcagcaguc     2520

aagcaagccu gggaagaugg uaucugcggg aucuccucug uuucaagaau ggaaaacauc     2580

auguggagau caguagaagg ggagcucaac gcaauccugg aagagaaugg aguucaacug     2640

acggucguug ugggaucugu aaaaaacccc auguggagag guccacagag auugcccgug     2700

ccugugaacg agcugcccca cggcuggaag gcuuggggga aaucguacuu cgucagagca     2760

gcaaagacaa auaacagcuu ugucguggau ggugacacac ugaaggaaug cccacucaaa     2820

cauagagcau ggaacagcuu ucuuguggag gaucaugggu ucgggguauu ucacacuagu     2880

gucuggcuca agguuagaga agauuauuca uuagagugug auccagccgu uauuggaaca     2940

gcuguuaagg gaaaggaggc uguacacagu gaucuaggcu acuggauuga gagugagaag     3000

aaugacacau ggaggcugaa gagggcccau cugaucgaga ugaaaacaug ugaauggcca     3060

aagucccaca cauuguggac agauggaaua gaagagagug aucugaucau acccaagucu     3120

uuagcugggc cacucagcca ucacaauacc agagagggcu acaggaccca aaugaaaggg     3180

ccauggcaca gugaagagcu ugaaauucgg uuugaggaau gcccaggcac uaagguccac     3240

guggaggaaa cauguggaac gagaggacca ucucugagau caaccacugc aagcggaagg     3300

gugaucgagg aauggugcug cagggagugc acaaugcccc cacugucguu ccgggcuaaa     3360

gauggcuguu gguauggaau ggagauaagg cccaggaaag aaccagaaag caacuuagua     3420

aggucaaugg ugacugcagg aucaacugau cacauggacc acuucucccu uggagugcuu     3480

gugauucugc ucauggugca ggaaggguug aagaagagaa ugaccacaaa gaucaucaua     3540

agcacaucaa uggcagugcu gguagcuaug auccugggag gauuuucaau gagugaccug     3600

gcuaagcuug caauuuugau gggugccacc uucgcggaaa ugaacacugg aggagaugua     3660

gcucaucugg cgcugauagc ggcauucaaa gucagaccag cguugcuggu aucuuucauc     3720

uucagagcua auuggacacc ccgugaaagc augcugcugg ccuuggccuc gugucuuuug     3780

caaacugcga ucuccgccuu ggaaggcgac cugaugguuc ucaucaaugg uuuugcuuug     3840

gccugguugg caauacgagc gaugguuguu ccacgcacug auaacaucac cuuggcaauc     3900

cuggcugcuc ugacaccacu ggcccggggc acacugcuug uggcguggag agcaggccuu     3960

gcuacuugcg ggggguuuau gcuccucucu cugaagggaa aaggcagugu gaagaagaac     4020

uuaccauuug ucauggcccu gggacuaacc gcugugaggc uggucgaccc caucaacgug     4080

gugggacugc uguugcucac aaggaguggg aagcggagcu ggcccccuag cgaaguacuc     4140

acagcuguug gccugauaug cgcauuggcu ggaggguucg ccaaggcaga uauagagaug     4200

gcugggccca uggccgcggu cggucugcua auugucaguu acguggucuc aggaaagagu     4260

guggacaugu acauugaaag agcaggugac aucacauggg aaaaagaugc ggaagucacu     4320

ggaaacaguc cccggcucga uguggcgcua gaugagagug gugauuucuc ccugguggag     4380

gaugacgguc cccccaugag agagaucaua cucaaggugg uccugaugac caucuguggc     4440

augaacccaa uagccauacc cuuugcagcu ggagcguggu acguauacgu gaagacugga     4500

aaaaggagug gugcucuaug ggaugugccu gcucccaagg aaguaaaaaa gggggagacc     4560

acagauggag uguacagagu aaugacucgu agacugcuag guucaacaca aguuggagug     4620

ggaguuaugc aagagggggu cuuucacacu auguggcacg ucacaaaagg auccgcgcug     4680

agaagcggug aagggagacu ugauccauac uggggagaug ucaagcagga ucugguguca     4740

uacugugguc cauggaagcu agaugccgcc ugggacgggc acagcgaggu gcagcucuug     4800

gccgugcccc ccggagagag agcgaggaac auccagacuc ugcccggaau auuuaagaca     4860

aaggaugggg acauuggagc gguugcgcug gauuacccag caggaacuuc aggaucuccu     4920

auccuagaca agugugggag agugauagga cuuuauggca auggggucgu gaucaaaaau     4980

gggaguuaug uuagugccau cacccaaggg aggagggagg aagagacucc uguugagugc     5040

uucgagccuu cgaugcugaa gaagaagcag cuaacugucu uagacuugca uccuggagcu     5100

gggaaaacca ggagaguucu uccugaaaua guccgugaag ccauaaaaac aagacuccgu     5160

acugugaucu uagcuccaac caggguuguc gcugcugaaa uggaggaggc ccuuagaggg     5220

cuuccagugc guuauaugac aacagcaguc aaugucaccc acucuggaac agaaaucguc     5280

gacuuaaugu gccaugccac cuucacuucg cgucuacuac agccaaucag aguccccaac     5340

uauaaucugu auauuaugga ugaggcccac uucacagauc ccucaaguau agcagcaaga     5400

ggauacauuu caacaagggu ugagaugggc gaggcggccg ccaucuucau gaccgccacg     5460

ccaccaggaa cccgugacgc auuuccggac uccaacucac caauuaugga caccgaagug     5520

gaagucccag agagagccug gagcucaggc uuugauuggg ugacggauca uucuggaaaa     5580

acaguuuggu uuguuccaag cgugaggaac ggcaaugaga ucgcagcuug ucugacaaag     5640

gcuggaaaac gggucauaca gcucagcaga aagacuuuug agacagaguu ccagaaaaca     5700

aaacaucaag agugggacuu ugucgugaca acugacauuu cagagauggg cgccaacuuu     5760

aaagcugacc gugucauaga uuccaggaga ugccuaaagc cggucauacu ugauggcgag     5820

agagucauuc uggcuggacc caugccuguc acacaugcca gcgcugccca gaggaggggg     5880

cgcauaggca ggaaucccaa caaaccugga gaugaguauc uguauggagg ugggugcgca     5940

gagacugacg aagaccaugc acacuggcuu gaagcaagaa ugcuccuuga caauauuuac     6000

cuccaagaug gccucauagc cucgcucuau cgaccugagg ccgacaaagu agcagccauu     6060

gagggagagu ucaagcuuag gacggagcaa aggaagaccu uuguggaacu caugaaaaga     6120

ggagaucuuc cuguuuggcu ggccuaucag guugcaucug ccggaauaac cuacacagau     6180

agaagauggu gcuuugaugg cacgaccaac aacaccauaa uggaagacag ugugccggca     6240

gaagugugga ccagacacgg agagaaaaga gugcucaaac cgagguggau ggacgccaga     6300

guuuguucag aucaugcggc ccugaaguca uucaaggagu uugccgcugg gaaaagagga     6360

gcggcuuuug gagugaugga agcccuggga acacugccag gacacaugac agagagauuc     6420

caggaagcca uugacaaccu cgcugugcuc augcgggcag agacuggaag caggccuuac     6480

aaagccgcgg cggcccaauu gccggagacc cuagagacca uuaugcuuuu gggguugcug     6540

ggaacagucu cgcugggaau cuucuucguc uugaugagga acaagggcau agggaagaug     6600

ggcuuuggaa uggugacucu uggggccagc gcauggcuca uguggcucuc ggaaauugag     6660

ccagccagaa uugcaugugu ccucauuguu guguuccuau ugcugguggu gcucauaccu     6720

gagccagaaa agcaaagauc uccccaggac aaccaaaugg caaucaucau caugguagca     6780

guaggucuuc ugggcuugau uaccgccaau gaacucggau gguuggagag aacaaagagu     6840

gaccuaagcc aucuaauggg aaggagagag gagggggcaa ccauaggauu cucaauggac     6900

auugaccugc ggccagccuc agcuugggcc aucuaugcug ccuugacaac uuucauuacc     6960

ccagccgucc aacaugcagu gaccaccuca uacaacaacu acuccuuaau ggcgauggcc     7020

acgcaagcug gaguguuguu ugguaugggc aaagggaugc cauucuacgc augggacuuu     7080

ggagucccgc ugcuaaugau agguugcuac ucacaauuaa caccccugac ccuaauagug     7140

gccaucauuu ugcucguggc gcacuacaug uacuugaucc cagggcugca ggcagcagcu     7200

gcgcgugcug cccagaagag aacggcagcu ggcaucauga agaacccugu uguggaugga     7260

auagugguga cugacauuga cacaaugaca auugaccccc aaguggagaa aaagauggga     7320

caggugcuac ucauagcagu agccgucucc agcgccauac ugucgcggac cgccuggggg     7380

uggggggagg cuggggcccu gaucacagcc gcaacuucca cuuuguggga aggcucuccg     7440

aacaaguacu ggaacuccuc uacagccacu ucacugugua acauuuuuag gggaaguuac     7500

uuggcuggag cuucucuaau cuacacagua acaagaaacg cuggcuuggu caagagacgu     7560

ggggguggaa caggagagac ccugggagag aaauggaagg cccgcuugaa ccagaugucg     7620

gcccuggagu ucuacuccua caaaaaguca ggcaucaccg aggugugcag agaagaggcc     7680

cgccgcgccc ucaaggacgg uguggcaacg ggaggccaug cugugucccg aggaagugca     7740

aagcugagau gguuggugga gcggggauac cugcagcccu auggaaaggu cauugaucuu     7800

ggauguggca gagggggcug gaguuacuac gccgccacca uccgcaaagu ucaagaagug     7860

aaaggauaca caaaaggagg cccuggucau gaagaacccg uguuggugca aagcuauggg     7920

uggaacauag uccgucuuaa gaguggggug gacgucuuuc auauggcggc ugagccgugu     7980

gacacguugc ugugugacau aggugaguca ucaucuaguc cugaagugga agaagcacgg     8040

acgcucagag uccucuccau ggugggggau uggcuugaaa aaagaccagg agccuuuugu     8100

auaaaagugu ugugcccaua caccagcacu augauggaaa cccuggagcg acugcagcgu     8160

agguaugggg gaggacuggu cagagugcca cucucccgca acucuacaca ugagauguac     8220

ugggucucug gagcgaaaag caacaccaua aaaagugugu ccaccacgag ccagcuccuc     8280

uuggggcgca uggacgggcc uaggaggcca gugaaauaug aggaggaugu gaaucucggc     8340

ucuggcacgc gggcuguggu aagcugcgcu gaagcuccca acaugaagau cauugguaac     8400

cgcauugaaa ggauccgcag ugagcacgcg gaaacguggu ucuuugacga gaaccaccca     8460

uauaggacau gggcuuacca uggaagcuau gaggccccca cacaaggguc agcguccucu     8520

cuaauaaacg ggguugucag gcuccuguca aaacccuggg auguggugac uggagucaca     8580

ggaauagcca ugaccgacac cacaccguau ggucagcaaa gaguuuucaa ggaaaaagug     8640

gacacuaggg ugccagaccc ccaagaaggc acucgucagg uuaugagcau ggucucuucc     8700

ugguugugga aagagcuagg caaacacaaa cggccacgag ucuguaccaa agaagaguuc     8760

aucaacaagg uucguagcaa ugcagcauua ggggcaauau uugaagagga aaaagagugg     8820

aagacugcag uggaagcugu gaacgaucca agguucuggg cucuagugga caaggaaaga     8880

gagcaccacc ugagaggaga gugccagagu uguguguaca acaugauggg aaaaagagaa     8940

aagaaacaag gggaauuugg aaaggccaag ggcagccgcg ccaucuggua uauguggcua     9000

ggggcuagau uucuagaguu cgaagcccuu ggauucuuga acgaggauca cuggaugggg     9060

agagagaacu caggaggugg uguugaaggg cugggauuac aaagacucgg auauguccua     9120

gaagagauga gucguauacc aggaggaagg auguaugcag augacacugc uggcugggac     9180

acccgcauua gcagguuuga ucuggagaau gaagcucuaa ucaccaacca aauggagaaa     9240

gggcacaggg ccuuggcauu ggccauaauc aaguacacau accaaaacaa agugguaaag     9300

guccuuagac cagcugaaaa agggaaaaca guuauggaca uuauuucgag acaagaccaa     9360

agggggagcg gacaaguugu cacuuacgcu cuuaacacau uuaccaaccu aguggugcaa     9420

cucauucgga auauggaggc ugaggaaguu cuagagaugc aagacuugug gcugcugcgg     9480

aggucagaga aagugacuaa cugguugcag agcaacggau gggauaggcu caaacgaaug     9540

gcagucagug gagaugauug cguugugaag ccaauugaug auagguuugc acaugcccuc     9600

agguucuuga augauauggg aaaaguuagg aaggacacac aagaguggaa acccucaacu     9660

ggaugggaca acugggaaga aguuccguuu ugcucccacc acuucaacaa gcuccaucuc     9720

aaggacggga gguccauugu gguucccugc cgccaccaag augaacugau uggccgggcc     9780

cgcgucucuc caggggcggg auggagcauc cgggagacug cuugccuagc aaaaucauau     9840

gcgcaaaugu ggcagcuccu uuauuuccac agaagggacc uccgacugau ggccaaugcc     9900

auuuguucau cugugccagu ugacuggguu ccaacuggga gaacuaccug gucaauccau     9960

ggaaagggag aauggaugac cacugaagac augcuugugg uguggaacag aguguggauu    10020

gaggagaacg accacaugga agacaagacc ccaguuacga aauggacaga cauucccuau    10080

uugggaaaaa gggaagacuu guggugugga ucucucauag ggcacagacc gcgcaccacc    10140

ugggcugaga acauuaaaaa cacagucaac auggugcgca ggaucauagg ugaugaagaa    10200

aaguacaugg acuaccuauc cacccaaguu cgcuacuugg gugaagaagg gucuacaccu    10260

ggagugcug                                                            10269


<210>  71
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C CDS mRNA wild type

<400>  71
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcgauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac ga                                                          312


<210>  72
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-ER anchor CDS mRNA wild type

<400>  72
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcgauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac gaggcgcaga uacuaguguc ggaauuguug gccuccugcu gaccacagcu      360

auggca                                                                 366


<210>  73
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) pr CDS mRNA wild type

<400>  73
gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg       60

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      120

cuuggacaca cgugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      180

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      240

ugccaucaca aaaaagguga agcacggaga ucuagaaga                             279


<210>  74
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) M CDS mRNA wild type

<400>  74
gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug       60

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      120

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      180

gucauauacu uggucaugau acugcugauu gccccggcau acagc                      225


<210>  75
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prM CDS mRNA wild type

<400>  75
gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg       60

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      120

cuuggacaca cgugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      180

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      240

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      300

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      360

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      420

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      480

cugcugauug ccccggcaua cagc                                             504


<210>  76
<211>  1512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) E CDS mRNA wild type

<400>  76
aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg       60

guugauguug ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu      120

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      180

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      240

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      300

uggggaaaug gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca      360

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug      420

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu      480

gaugagaaua gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug      540

gggggguuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau      600

uuguauuacu ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac      660

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa      720

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu      780

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca      840

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug      900

aagggcgugu cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa      960

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1020

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1080

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1140

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1200

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1260

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1320

uugggcaagg gcauccauca aaucuuugga gcagcuuuca aaucauuguu uggaggaaug     1380

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacgcaaag     1440

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     1500

gccgucucug cu                                                         1512


<210>  77
<211>  2016
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME CDS mRNA wild type

<400>  77
gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg       60

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      120

cuuggacaca cgugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      180

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      240

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      300

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      360

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      420

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      480

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      540

gaagguaugu caggugggac uuggguugau guugucuugg aacauggagg uugugucacu      600

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      660

auggcggagg uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc      720

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      780

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagc      840

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      900

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc      960

guuaaugaca caggacauga aacugaugag aauagagcga aaguugagau aacgcccaau     1020

ucaccaagag ccgaagccac ccuggggggg uuuggaagcc uaggacuuga uugugaaccg     1080

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacuggcug     1140

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1200

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1260

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1320

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1380

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac ugcagcguuc     1440

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1500

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1560

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1620

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1680

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1740

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1800

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaaucuu uggagcagcu     1860

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1920

augugguugg gucugaacgc aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     1980

ggaguguuga ucuucuuauc cacagccguc ucugcu                               2016


<210>  78
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS1 CDS mRNA wild type

<400>  78
gauguggggu gcucggugga cuucucaaag aaggagacga gaugcgguac agggguguuc       60

gucuauaacg acguugaagc cuggagggac agguacaagu accauccuga cuccccccgu      120

agauuggcag cagcagucaa gcaagccugg gaagauggua ucugcgggau cuccucuguu      180

ucaagaaugg aaaacaucau guggagauca guagaagggg agcucaacgc aauccuggaa      240

gagaauggag uucaacugac ggucguugug ggaucuguaa aaaaccccau guggagaggu      300

ccacagagau ugcccgugcc ugugaacgag cugccccacg gcuggaaggc uugggggaaa      360

ucguacuucg ucagagcagc aaagacaaau aacagcuuug ucguggaugg ugacacacug      420

aaggaaugcc cacucaaaca uagagcaugg aacagcuuuc uuguggagga ucauggguuc      480

gggguauuuc acacuagugu cuggcucaag guuagagaag auuauucauu agagugugau      540

ccagccguua uuggaacagc uguuaaggga aaggaggcug uacacaguga ucuaggcuac      600

uggauugaga gugagaagaa ugacacaugg aggcugaaga gggcccaucu gaucgagaug      660

aaaacaugug aauggccaaa gucccacaca uuguggacag auggaauaga agagagugau      720

cugaucauac ccaagucuuu agcugggcca cucagccauc acaauaccag agagggcuac      780

aggacccaaa ugaaagggcc auggcacagu gaagagcuug aaauucgguu ugaggaaugc      840

ccaggcacua agguccacgu ggaggaaaca uguggaacga gaggaccauc ucugagauca      900

accacugcaa gcggaagggu gaucgaggaa uggugcugca gggagugcac aaugccccca      960

cugucguucc gggcuaaaga uggcuguugg uauggaaugg agauaaggcc caggaaagaa     1020

ccagaaagca acuuaguaag gucaauggug acugca                               1056


<210>  79
<211>  678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS2A CDS mRNA wild type

<400>  79
ggaucaacug aucacaugga ccacuucucc cuuggagugc uugugauucu gcucauggug       60

caggaagggu ugaagaagag aaugaccaca aagaucauca uaagcacauc aauggcagug      120

cugguagcua ugauccuggg aggauuuuca augagugacc uggcuaagcu ugcaauuuug      180

augggugcca ccuucgcgga aaugaacacu ggaggagaug uagcucaucu ggcgcugaua      240

gcggcauuca aagucagacc agcguugcug guaucuuuca ucuucagagc uaauuggaca      300

ccccgugaaa gcaugcugcu ggccuuggcc ucgugucuuu ugcaaacugc gaucuccgcc      360

uuggaaggcg accugauggu ucucaucaau gguuuugcuu uggccugguu ggcaauacga      420

gcgaugguug uuccacgcac ugauaacauc accuuggcaa uccuggcugc ucugacacca      480

cuggcccggg gcacacugcu uguggcgugg agagcaggcc uugcuacuug cgggggguuu      540

augcuccucu cucugaaggg aaaaggcagu gugaagaaga acuuaccauu ugucauggcc      600

cugggacuaa ccgcugugag gcuggucgac cccaucaacg uggugggacu gcuguugcuc      660

acaaggagug ggaagcgg                                                    678


<210>  80
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS2B CDS mRNA wild type

<400>  80
agcuggcccc cuagcgaagu acucacagcu guuggccuga uaugcgcauu ggcuggaggg       60

uucgccaagg cagauauaga gauggcuggg cccauggccg cggucggucu gcuaauuguc      120

aguuacgugg ucucaggaaa gaguguggac auguacauug aaagagcagg ugacaucaca      180

ugggaaaaag augcggaagu cacuggaaac aguccccggc ucgauguggc gcuagaugag      240

aguggugauu ucucccuggu ggaggaugac ggucccccca ugagagagau cauacucaag      300

gugguccuga ugaccaucug uggcaugaac ccaauagcca uacccuuugc agcuggagcg      360

ugguacguau acgugaagac uggaaaaagg                                       390


<210>  81
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS3 CDS mRNA wild type

<400>  81
aguggugcuc uaugggaugu gccugcuccc aaggaaguaa aaaaggggga gaccacagau       60

ggaguguaca gaguaaugac ucguagacug cuagguucaa cacaaguugg agugggaguu      120

augcaagagg gggucuuuca cacuaugugg cacgucacaa aaggauccgc gcugagaagc      180

ggugaaggga gacuugaucc auacugggga gaugucaagc aggaucuggu gucauacugu      240

gguccaugga agcuagaugc cgccugggac gggcacagcg aggugcagcu cuuggccgug      300

ccccccggag agagagcgag gaacauccag acucugcccg gaauauuuaa gacaaaggau      360

ggggacauug gagcgguugc gcuggauuac ccagcaggaa cuucaggauc uccuauccua      420

gacaagugug ggagagugau aggacuuuau ggcaaugggg ucgugaucaa aaaugggagu      480

uauguuagug ccaucaccca agggaggagg gaggaagaga cuccuguuga gugcuucgag      540

ccuucgaugc ugaagaagaa gcagcuaacu gucuuagacu ugcauccugg agcugggaaa      600

accaggagag uucuuccuga aauaguccgu gaagccauaa aaacaagacu ccguacugug      660

aucuuagcuc caaccagggu ugucgcugcu gaaauggagg aggcccuuag agggcuucca      720

gugcguuaua ugacaacagc agucaauguc acccacucug gaacagaaau cgucgacuua      780

augugccaug ccaccuucac uucgcgucua cuacagccaa ucagaguccc caacuauaau      840

cuguauauua uggaugaggc ccacuucaca gaucccucaa guauagcagc aagaggauac      900

auuucaacaa ggguugagau gggcgaggcg gccgccaucu ucaugaccgc cacgccacca      960

ggaacccgug acgcauuucc ggacuccaac ucaccaauua uggacaccga aguggaaguc     1020

ccagagagag ccuggagcuc aggcuuugau ugggugacgg aucauucugg aaaaacaguu     1080

ugguuuguuc caagcgugag gaacggcaau gagaucgcag cuugucugac aaaggcugga     1140

aaacggguca uacagcucag cagaaagacu uuugagacag aguuccagaa aacaaaacau     1200

caagaguggg acuuugucgu gacaacugac auuucagaga ugggcgccaa cuuuaaagcu     1260

gaccguguca uagauuccag gagaugccua aagccgguca uacuugaugg cgagagaguc     1320

auucuggcug gacccaugcc ugucacacau gccagcgcug cccagaggag ggggcgcaua     1380

ggcaggaauc ccaacaaacc uggagaugag uaucuguaug gaggugggug cgcagagacu     1440

gacgaagacc augcacacug gcuugaagca agaaugcucc uugacaauau uuaccuccaa     1500

gauggccuca uagccucgcu cuaucgaccu gaggccgaca aaguagcagc cauugaggga     1560

gaguucaagc uuaggacgga gcaaaggaag accuuugugg aacucaugaa aagaggagau     1620

cuuccuguuu ggcuggccua ucagguugca ucugccggaa uaaccuacac agauagaaga     1680

uggugcuuug auggcacgac caacaacacc auaauggaag acagugugcc ggcagaagug     1740

uggaccagac acggagagaa aagagugcuc aaaccgaggu ggauggacgc cagaguuugu     1800

ucagaucaug cggcccugaa gucauucaag gaguuugccg cugggaaaag a              1851


<210>  82
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS4A CDS mRNA wild type

<400>  82
ggagcggcuu uuggagugau ggaagcccug ggaacacugc caggacacau gacagagaga       60

uuccaggaag ccauugacaa ccucgcugug cucaugcggg cagagacugg aagcaggccu      120

uacaaagccg cggcggccca auugccggag acccuagaga ccauuaugcu uuugggguug      180

cugggaacag ucucgcuggg aaucuucuuc gucuugauga ggaacaaggg cauagggaag      240

augggcuuug gaauggugac ucuuggggcc agcgcauggc ucauguggcu cucggaaauu      300

gagccagcca gaauugcaug uguccucauu guuguguucc uauugcuggu ggugcucaua      360

ccugagccag aaaagcaaag aucuccccag gacaaccaaa uggcaaucau caucauggua      420

gcaguagguc uucugggcuu gauuaccgcc                                       450


<210>  83
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS4B CDS mRNA wild type

<400>  83
aaugaacucg gaugguugga gagaacaaag agugaccuaa gccaucuaau gggaaggaga       60

gaggaggggg caaccauagg auucucaaug gacauugacc ugcggccagc cucagcuugg      120

gccaucuaug cugccuugac aacuuucauu accccagccg uccaacaugc agugaccacc      180

ucauacaaca acuacuccuu aauggcgaug gccacgcaag cuggaguguu guuugguaug      240

ggcaaaggga ugccauucua cgcaugggac uuuggagucc cgcugcuaau gauagguugc      300

uacucacaau uaacaccccu gacccuaaua guggccauca uuuugcucgu ggcgcacuac      360

auguacuuga ucccagggcu gcaggcagca gcugcgcgug cugcccagaa gagaacggca      420

gcuggcauca ugaagaaccc uguuguggau ggaauagugg ugacugacau ugacacaaug      480

acaauugacc cccaagugga gaaaaagaug ggacaggugc uacucauagc aguagccguc      540

uccagcgcca uacugucgcg gaccgccugg gggugggggg aggcuggggc ccugaucaca      600

gccgcaacuu ccacuuugug ggaaggcucu ccgaacaagu acuggaacuc cucuacagcc      660

acuucacugu guaacauuuu uaggggaagu uacuuggcug gagcuucucu aaucuacaca      720

guaacaagaa acgcuggcuu ggucaagaga cgu                                   753


<210>  84
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS5 CDS mRNA wild type

<400>  84
ggggguggaa caggagagac ccugggagag aaauggaagg cccgcuugaa ccagaugucg       60

gcccuggagu ucuacuccua caaaaaguca ggcaucaccg aggugugcag agaagaggcc      120

cgccgcgccc ucaaggacgg uguggcaacg ggaggccaug cugugucccg aggaagugca      180

aagcugagau gguuggugga gcggggauac cugcagcccu auggaaaggu cauugaucuu      240

ggauguggca gagggggcug gaguuacuac gccgccacca uccgcaaagu ucaagaagug      300

aaaggauaca caaaaggagg cccuggucau gaagaacccg uguuggugca aagcuauggg      360

uggaacauag uccgucuuaa gaguggggug gacgucuuuc auauggcggc ugagccgugu      420

gacacguugc ugugugacau aggugaguca ucaucuaguc cugaagugga agaagcacgg      480

acgcucagag uccucuccau ggugggggau uggcuugaaa aaagaccagg agccuuuugu      540

auaaaagugu ugugcccaua caccagcacu augauggaaa cccuggagcg acugcagcgu      600

agguaugggg gaggacuggu cagagugcca cucucccgca acucuacaca ugagauguac      660

ugggucucug gagcgaaaag caacaccaua aaaagugugu ccaccacgag ccagcuccuc      720

uuggggcgca uggacgggcc uaggaggcca gugaaauaug aggaggaugu gaaucucggc      780

ucuggcacgc gggcuguggu aagcugcgcu gaagcuccca acaugaagau cauugguaac      840

cgcauugaaa ggauccgcag ugagcacgcg gaaacguggu ucuuugacga gaaccaccca      900

uauaggacau gggcuuacca uggaagcuau gaggccccca cacaaggguc agcguccucu      960

cuaauaaacg ggguugucag gcuccuguca aaacccuggg auguggugac uggagucaca     1020

ggaauagcca ugaccgacac cacaccguau ggucagcaaa gaguuuucaa ggaaaaagug     1080

gacacuaggg ugccagaccc ccaagaaggc acucgucagg uuaugagcau ggucucuucc     1140

ugguugugga aagagcuagg caaacacaaa cggccacgag ucuguaccaa agaagaguuc     1200

aucaacaagg uucguagcaa ugcagcauua ggggcaauau uugaagagga aaaagagugg     1260

aagacugcag uggaagcugu gaacgaucca agguucuggg cucuagugga caaggaaaga     1320

gagcaccacc ugagaggaga gugccagagu uguguguaca acaugauggg aaaaagagaa     1380

aagaaacaag gggaauuugg aaaggccaag ggcagccgcg ccaucuggua uauguggcua     1440

ggggcuagau uucuagaguu cgaagcccuu ggauucuuga acgaggauca cuggaugggg     1500

agagagaacu caggaggugg uguugaaggg cugggauuac aaagacucgg auauguccua     1560

gaagagauga gucguauacc aggaggaagg auguaugcag augacacugc uggcugggac     1620

acccgcauua gcagguuuga ucuggagaau gaagcucuaa ucaccaacca aauggagaaa     1680

gggcacaggg ccuuggcauu ggccauaauc aaguacacau accaaaacaa agugguaaag     1740

guccuuagac cagcugaaaa agggaaaaca guuauggaca uuauuucgag acaagaccaa     1800

agggggagcg gacaaguugu cacuuacgcu cuuaacacau uuaccaaccu aguggugcaa     1860

cucauucgga auauggaggc ugaggaaguu cuagagaugc aagacuugug gcugcugcgg     1920

aggucagaga aagugacuaa cugguugcag agcaacggau gggauaggcu caaacgaaug     1980

gcagucagug gagaugauug cguugugaag ccaauugaug auagguuugc acaugcccuc     2040

agguucuuga augauauggg aaaaguuagg aaggacacac aagaguggaa acccucaacu     2100

ggaugggaca acugggaaga aguuccguuu ugcucccacc acuucaacaa gcuccaucuc     2160

aaggacggga gguccauugu gguucccugc cgccaccaag augaacugau uggccgggcc     2220

cgcgucucuc caggggcggg auggagcauc cgggagacug cuugccuagc aaaaucauau     2280

gcgcaaaugu ggcagcuccu uuauuuccac agaagggacc uccgacugau ggccaaugcc     2340

auuuguucau cugugccagu ugacuggguu ccaacuggga gaacuaccug gucaauccau     2400

ggaaagggag aauggaugac cacugaagac augcuugugg uguggaacag aguguggauu     2460

gaggagaacg accacaugga agacaagacc ccaguuacga aauggacaga cauucccuau     2520

uugggaaaaa gggaagacuu guggugugga ucucucauag ggcacagacc gcgcaccacc     2580

ugggcugaga acauuaaaaa cacagucaac auggugcgca ggaucauagg ugaugaagaa     2640

aaguacaugg acuaccuauc cacccaaguu cgcuacuugg gugaagaagg gucuacaccu     2700

ggagugcug                                                             2709


<210>  85
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS mRNA wild type

<400>  85
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca cgugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacu guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaaucuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacgc aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  86
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS mRNA wild type

<400>  86
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacgag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca cgugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacu guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaaucuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacgc aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  87
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS mRNA wild type

<400>  87
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacu guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  88
<211>  10257
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein CDS mRNA wild type

<400>  88
augaaaaacc ccaaagaaga aauccggagg auccggauug ucaauaugcu aaaacgcgga       60

guagcccgug uaaaccccuu gggagguuug aagagguugc cagccggacu ucugcugggu      120

cauggaccca ucagaauggu uuuggcgaua cuagccuuuu ugagauuuac agcaaucaag      180

ccaucacugg gccuuaucaa cagauggggu uccgugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuugcugcc auguugagaa uaaucaaugc uaggaaagag      300

aggaagagac guggcgcaga caccagcauc ggaaucauug gccuccugcu gacuacagcc      360

auggcagcag agaucacuag acgcgggagu gcauacuaca uguacuugga uaggagcgau      420

gccgggaagg ccauuucguu ugcuaccaca uugggaguga acaagugcca cguacagauc      480

auggaccucg ggcacaugug ugacgccacc augaguuaug agugcccuau gcuggaugag      540

ggaguggaac cagaugaugu cgauugcugg ugcaacacga caucaacuug gguuguguac      600

ggaaccuguc aucacaaaaa aggugaggca cggcgaucua gaagagccgu gacgcucccu      660

ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu gguuagaauc aagagaauac      720

acgaagcacu ugaucaaggu ugaaaacugg auauucagga accccggguu ugcgcuagug      780

gccguugcca uugccuggcu uuugggaagc ucgacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagu aucaggugca uuggagucag caauagagac      900

uucguggagg gcaugucagg ugggaccugg guugauguug ucuuggaaca uggaggcugc      960

guuaccguga uggcacagga caagccaaca gucgacauag aguuggucac gacgacgguu     1020

aguaacaugg ccgagguaag auccuauugc uacgaggcau cgauaucgga cauggcuucg     1080

gacagucguu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacauuagu ggacagaggu uggggaaacg guuguggacu uuuuggcaaa     1200

gggagcuugg ugacaugugc caaguuuacg uguucuaaga agaugaccgg gaagagcauu     1260

caaccggaaa aucuggagua ucggauaaug cuaucagugc auggcuccca gcauagcggg     1320

augauuggau augaaacuga cgaagauaga gcgaaagucg agguuacgcc uaauucacca     1380

agagcggaag caaccuuggg aggcuuugga agcuuaggac uugacuguga accaaggaca     1440

ggccuugacu uuucagaucu guauuaccug accaugaaca auaagcauug guuggugcac     1500

aaagaguggu uucaugacau cccauugccu uggcaugcug gggcagacac cggaacucca     1560

cacuggaaca acaaagaggc auugguagaa uucaaggaug cccacgccaa gaggcaaacc     1620

gucgucguuc uggggagcca ggaaggagcc guucacacgg cucucgcugg agcucuagag     1680

gcugagaugg auggugcaaa gggaaggcug uucucuggcc auuugaaaug ccgccuaaaa     1740

auggacaagc uuagauugaa gggcguguca uauuccuugu gcacugcggc auucacauuc     1800

accaaggucc cagcugaaac acugcaugga acagucacag uggaggugca guaugcaggg     1860

acagauggac ccugcaagau cccaguccag auggcggugg acaugcagac ccugacccca     1920

guuggaaggc ugauaaccgc caaccccgug auuacugaaa gcacugagaa cucaaagaug     1980

auguuggagc uugacccacc auuuggggau ucuuacauug ucauaggagu uggggacaag     2040

aaaaucaccc accacuggca uaggaguggu agcaccaucg gaaaggcauu ugaggccacu     2100

gugagaggcg ccaagagaau ggcaguccug ggggauacag ccugggacuu cggaucaguc     2160

gggggugugu ucaacucacu ggguaagggc auucaccaga uuuuuggagc agccuucaaa     2220

ucacuguuug gaggaauguc cugguucuca cagauccuca uaggcacgcu gcuagugugg     2280

uuagguuuga acacaaagaa uggaucuauc ucccucacau gcuuggcccu ggggggagug     2340

augaucuucc ucuccacggc uguuucugcu gacguggggu gcucagugga cuucucaaaa     2400

aaggaaacga gauguggcac ggggguauuc aucuauaaug auguugaagc cuggagggac     2460

cgguacaagu accauccuga cuccccccgc agauuggcag cagcagucaa gcaggccugg     2520

gaagagggga ucugugggau cucauccguu ucaagaaugg aaaacaucau guggaaauca     2580

guagaagggg agcucaaugc uauccuagag gagaauggag uucaacugac aguuguugug     2640

ggaucuguaa aaaaccccau guggagaggu ccacaaagau ugccagugcc ugugaaugag     2700

cugccccaug gcuggaaagc cugggggaaa ucguauuuug uuagggcggc aaagaccaac     2760

aacaguuuug uugucgacgg ugacacacug aaggaauguc cgcuugagca cagagcaugg     2820

aauaguuuuc uuguggagga ucacggguuu ggagucuucc acaccagugu cuggcuuaag     2880

gucagagaag auuacucauu agaaugugac ccagccguca uaggaacagc uguuaaggga     2940

agggaggccg cgcacaguga ucugggcuau uggauugaaa gugaaaagaa ugacacaugg     3000

aggcugaaga gggcccaccu gauugagaug aaaacaugug aauggccaaa gucucacaca     3060

uuguggacag auggaguaga agaaagugau cuuaucauac ccaagucuuu agcuggucca     3120

cucagccacc acaacaccag agaggguuac agaacccaag ugaaagggcc auggcacagu     3180

gaagagcuug aaauccgguu ugaggaaugu ccaggcacca agguuuacgu ggaggagaca     3240

ugcggaacua gaggaccauc ucugagauca acuacugcaa guggaagggu cauugaggaa     3300

uggugcugua gggaaugcac aaugccccca cuaucguuuc gagcaaaaga cggcugcugg     3360

uauggaaugg agauaaggcc caggaaagaa ccagagagca acuuagugag gucaauggug     3420

acagcggggu caaccgauca uauggaccac uucucucuug gagugcuugu gauucuacuc     3480

auggugcagg agggguugaa gaagagaaug accacaaaga ucaucaugag cacaucaaug     3540

gcagugcugg uagucaugau cuugggagga uuuucaauga gugaccuggc caagcuugug     3600

auccugaugg gugcuacuuu cgcagaaaug aacacuggag gagauguagc ucacuuggca     3660

uugguagcgg cauuuaaagu cagaccagcc uugcuggucu ccuucauuuu cagagccaau     3720

uggacacccc gugagagcau gcugcuagcc cuggcuucgu gucuucugca aacugcgauc     3780

ucugcucuug aaggugacuu gaugguccuc auuaauggau uugcuuuggc cugguuggca     3840

auucgagcaa uggccgugcc acgcacugac aacaucgcuc uaccaaucuu ggcugcucua     3900

acaccacuag cucgaggcac acugcucgug gcauggagag cgggccuggc uacuugugga     3960

gggaucaugc uccucucccu gaaagggaaa gguaguguga agaagaaccu gccauuuguc     4020

auggcccugg gauugacagc ugugagggua guagacccua uuaauguggu aggacuacug     4080

uuacucacaa ggagugggaa gcggagcugg cccccuagug aaguucucac agccguuggc     4140

cugauaugug cacuggccgg aggguuugcc aaggcagaca uugagauggc uggacccaug     4200

gcugcaguag gcuugcuaau ugucagcuau guggucucgg gaaagagugu ggacauguac     4260

auugaaagag caggugacau cacaugggaa aaggacgcgg aagucacugg aaacaguccu     4320

cggcuugacg uggcacugga ugagaguggu gacuucuccu ugguagagga agauggucca     4380

cccaugagag agaucauacu caaggugguc cugauggcca ucuguggcau gaacccaaua     4440

gcuauaccuu uugcugcagg agcgugguau guguauguga agacugggaa aaggaguggc     4500

gcccucuggg acgugccugc ucccaaagaa gugaagaaag gagagaccac agauggagug     4560

uacagaguga ugacucgcag acugcuaggu ucaacacagg uuggaguggg agucaugcaa     4620

gagggagucu uccacaccau guggcacguu acaaaaggag ccgcacugag gagcggugag     4680

ggaagacuug auccauacug gggggauguc aagcaggacu uggugucaua cugugggccu     4740

uggaaguugg augcagcuug ggauggacuc agcgagguac agcuuuuggc cguaccuccc     4800

ggagagaggg ccagaaacau ucagacccug ccuggaauau ucaagacaaa ggacggggac     4860

aucggagcag uugcucugga cuacccugca gggaccucag gaucuccgau ccuagacaaa     4920

uguggaagag ugauaggacu cuauggcaau gggguuguga ucaagaaugg aagcuauguu     4980

agugcuauaa cccagggaaa gagggaggag gagacuccgg uugaauguuu cgaacccucg     5040

augcugaaga agaagcagcu aacugucuug gaucugcauc caggagccgg aaaaaccagg     5100

agaguucuuc cugaaauagu ccgugaagcc auaaaaaaga gacuccggac agugaucuug     5160

gcaccaacua ggguugucgc ugcugagaug gaggaggccu ugagaggacu uccggugcgu     5220

uacaugacaa cagcagucaa cgucacccau ucugggacag aaaucguuga uuugaugugc     5280

caugccacuu ucacuucacg cuuacuacaa cccaucagag ucccuaauua caaucucaac     5340

aucauggaug aagcccacuu cacagacccc ucaaguauag cugcaagagg auacauauca     5400

acaaggguug aaaugggcga ggcggcugcc auuuuuauga cugccacacc accaggaacc     5460

cgugaugcgu uuccugacuc uaacucacca aucauggaca cagaagugga agucccagag     5520

agagccugga gcucaggcuu ugauugggug acagaccauu cugggaaaac aguuugguuc     5580

guuccaagcg ugagaaacgg aaaugaaauc gcagccuguc ugacaaaggc uggaaagcgg     5640

gucauacagc ucagcaggaa gacuuuugag acagaauuuc agaaaacaaa aaaucaagag     5700

ugggacuuug ucauaacaac ugacaucuca gagaugggcg ccaacuucaa ggcugaccgg     5760

gucauagacu cuaggagaug ccuaaaacca gucauacuug auggugagag agucaucuug     5820

gcugggccca ugccugucac gcaugcuagu gcugcucaga ggagaggacg uauaggcagg     5880

aacccuaaca aaccuggaga ugaguacaug uauggaggug ggugugcaga gacugaugaa     5940

ggccaugcac acuggcuuga agcaagaaug cuucuugaca acaucuaccu ccaggauggc     6000

cucauagccu cgcucuaucg gccugaggcc gauaagguag ccgccauuga gggagaguuu     6060

aagcugagga cagagcaaag gaagaccuuc guggaacuca ugaagagagg agaccuuccc     6120

gucuggcuag ccuaucaggu ugcaucugcc ggaauaacuu acacagacag aagauggugc     6180

uuugauggca caaccaacaa caccauaaug gaagacagug uaccagcaga gguuuggaca     6240

aaguauggag agaagagagu gcucaaaccg agauggaugg augcuagggu cuguucagac     6300

caugcggccc ugaagucguu caaagaauuc gccgcuggaa aaagaggagc ggcuuuggga     6360

guaauggagg cccugggaac acugccagga cacaugacag agagguuuca ggaagccauu     6420

gacaaccucg ccgugcucau gcgagcagag acuggaagca ggccuuauaa ggcagcggca     6480

gcccaacugc cggagacccu agagaccauu augcucuuag guuugcuggg aacaguuuca     6540

cuggggaucu ucuucgucuu gaugcggaau aagggcaucg ggaagauggg cuuuggaaug     6600

guaacccuug gggccagugc auggcucaug uggcuuucgg aaauugaacc agccagaauu     6660

gcaugugucc ucauuguugu guuuuuauua cugguggugc ucauacccga gccagagaag     6720

caaagaucuc cccaagauaa ccagauggca auuaucauca ugguggcagu gggccuucua     6780

gguuugauaa cugcaaacga acuuggaugg cuggaaagaa caaaaaauga cauagcucau     6840

cuaaugggaa ggagagaaga aggagcaacc augggauucu caauggacau ugaucugcgg     6900

ccagccuccg ccugggcuau cuaugccgca uugacaacuc ucaucacccc agcuguccaa     6960

caugcgguaa ccacuucaua caacaacuac uccuuaaugg cgauggccac acaagcugga     7020

gugcuguuug gcaugggcaa agggaugcca uuuaugcaug gggaccuugg agucccgcug     7080

cuaaugaugg guugcuauuc acaauuaaca ccccugacuc ugauaguagc uaucauucug     7140

cuuguggcgc acuacaugua cuugauccca ggccuacaag cggcagcagc gcgugcugcc     7200

cagaaaagga cagcagcugg caucaugaag aaucccguug uggauggaau agugguaacu     7260

gacauugaca caaugacaau agacccccag guggagaaga agaugggaca aguguuacuc     7320

auagcaguag ccaucuccag ugcugugcug cugcggaccg ccuggggaug gggggaggcu     7380

ggagcucuga ucacagcagc gaccuccacc uugugggaag gcucuccaaa caaauacugg     7440

aacuccucua cagccaccuc acugugcaac aucuucagag gaagcuaucu ggcaggagcu     7500

ucccuuaucu auacagugac gagaaacgcu ggccugguua agagacgugg aggugggacg     7560

ggagagacuc ugggagagaa guggaaagcu cgucugaauc agaugucggc ccuggaguuc     7620

uacucuuaua aaaagucagg uaucacugaa guguguagag aggaggcucg ccgugcccuc     7680

aaggauggag uggccacagg aggacaugcc guaucccggg gaagugcaaa gaucagaugg     7740

uuggaggaga gaggauaucu gcagcccuau gggaagguug uugaccucgg auguggcaga     7800

gggggcugga gcuauuaugc cgccaccauc cgcaaagugc aggaggugag aggauacaca     7860

aagggagguc ccggucauga agaacccaug cuggugcaaa gcuaugggug gaacauaguu     7920

cgucucaaga guggagugga cgucuuccac auggcggcug agccguguga cacucugcug     7980

ugugacauag gugagucauc aucuaguccu gaaguggaag agacacgaac acucagagug     8040

cucucuaugg ugggggacug gcuugaaaaa agaccagggg ccuucuguau aaaggugcug     8100

ugcccauaca ccagcacuau gauggaaacc auggagcgac ugcaacguag gcauggggga     8160

ggauuaguca gagugccauu gugucgcaac uccacacaug agauguacug ggucucuggg     8220

gcaaagagca acaucauaaa aagugugucc accacaaguc agcuccuccu gggacgcaug     8280

gauggcccca ggaggccagu gaaauaugag gaggauguga accucggcuc ggguacacga     8340

gcuguggcaa gcugugcuga ggcuccuaac augaaaauca ucggcaggcg cauugagaga     8400

auccgcaaug aacaugcaga aacaugguuu cuugaugaaa accacccaua caggacaugg     8460

gccuaccaug ggagcuacga agcccccacg caaggaucag cgucuucccu cgugaacggg     8520

guuguuagac uccugucaaa gccuugggac guggugacug gaguuacagg aauagccaug     8580

acugacacca caccauacgg ccaacaaaga gucuucaaag aaaaagugga caccagggug     8640

ccagaucccc aagaaggcac ucgccaggua augaacauag ucucuuccug gcuguggaag     8700

gagcugggga aacgcaagcg gccacgcguc ugcaccaaag aagaguuuau caacaaggug     8760

cgcagcaaug cagcacuggg agcaauauuu gaagaggaaa aagaauggaa gacggcugug     8820

gaagcuguga augauccaag guuuugggcc cuaguggaua gggagagaga acaccaccug     8880

agaggagagu gucacagcug uguguacaac augaugggaa aaagagaaaa gaagcaagga     8940

gaguucggga aagcaaaagg uagccgcgcc aucugguaca ugugguuggg agccagauuc     9000

uuggaguuug aagcccuugg auucuugaac gaggaccauu ggaugggaag agaaaacuca     9060

ggagguggag ucgaaggguu aggauugcaa agacuuggau acauucuaga agaaaugaau     9120

cgggcaccag gaggaaagau guacgcagau gacacugcug gcugggacac ccgcauuagu     9180

aaguuugauc uggagaauga agcucugauu accaaccaaa uggaggaagg gcacagaacu     9240

cuggcguugg ccgugauuaa auacacauac caaaacaaag uggugaaggu ucucagacca     9300

gcugaaggag gaaaaacagu uauggacauc auuucaagac aagaccagag agggagugga     9360

caaguuguca cuuaugcucu caacacauuc accaacuugg uggugcagcu uauccggaac     9420

auggaagcug aggaaguguu agagaugcaa gacuuauggu uguugaggaa gccagagaaa     9480

gugaccagau gguugcagag caauggaugg gauagacuca aacgaauggc ggucagugga     9540

gaugacugcg uugugaagcc aaucgaugau agguuugcac augcccucag guucuugaau     9600

gacaugggaa aaguuaggaa agacacacag gaguggaaac ccucgacugg auggagcaau     9660

ugggaagaag ucccguucug cucccaccac uucaacaagc uguaccucaa ggaugggaga     9720

uccauugugg ucccuugccg ccaccaagau gaacugauug gccgagcucg cgucucacca     9780

ggggcaggau ggagcauccg ggagacugcc ugucuugcaa aaucauaugc gcagaugugg     9840

cagcuccuuu auuuccacag aagagaccuu cgacugaugg cuaaugccau uugcucggcu     9900

gugccaguug acuggguacc aacugggaga accaccuggu caauccaugg aaagggagaa     9960

uggaugacca cugaggacau gcucauggug uggaauagag uguggauuga ggagaacgac    10020

cauauggagg acaagacucc uguaacaaaa uggacagaca uucccuaucu aggaaaaagg    10080

gaggacuuau gguguggauc ccuuauaggg cacagacccc gcaccacuug ggcugaaaac    10140

aucaaagaca cagucaacau ggugcgcagg aucauaggug augaagaaaa guacauggac    10200

uaucuaucca cccaaguccg cuacuugggu gaggaagggu ccacacccgg aguguug       10257


<210>  89
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C CDS mRNA wild type

<400>  89
augaaaaacc ccaaagaaga aauccggagg auccggauug ucaauaugcu aaaacgcgga       60

guagcccgug uaaaccccuu gggagguuug aagagguugc cagccggacu ucugcugggu      120

cauggaccca ucagaauggu uuuggcgaua cuagccuuuu ugagauuuac agcaaucaag      180

ccaucacugg gccuuaucaa cagauggggu uccgugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuugcugcc auguugagaa uaaucaaugc uaggaaagag      300

aggaagagac gu                                                          312


<210>  90
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-ER anchor CDS mRNA wild type

<400>  90
augaaaaacc ccaaagaaga aauccggagg auccggauug ucaauaugcu aaaacgcgga       60

guagcccgug uaaaccccuu gggagguuug aagagguugc cagccggacu ucugcugggu      120

cauggaccca ucagaauggu uuuggcgaua cuagccuuuu ugagauuuac agcaaucaag      180

ccaucacugg gccuuaucaa cagauggggu uccgugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuugcugcc auguugagaa uaaucaaugc uaggaaagag      300

aggaagagac guggcgcaga caccagcauc ggaaucauug gccuccugcu gacuacagcc      360

auggca                                                                 366


<210>  91
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) pr CDS mRNA wild type

<400>  91
gcagagauca cuagacgcgg gagugcauac uacauguacu uggauaggag cgaugccggg       60

aaggccauuu cguuugcuac cacauuggga gugaacaagu gccacguaca gaucauggac      120

cucgggcaca ugugugacgc caccaugagu uaugagugcc cuaugcugga ugagggagug      180

gaaccagaug augucgauug cuggugcaac acgacaucaa cuuggguugu guacggaacc      240

ugucaucaca aaaaagguga ggcacggcga ucuagaaga                             279


<210>  92
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) M CDS mRNA wild type

<400>  92
gccgugacgc ucccuucuca cucuacaagg aaguugcaaa cgcggucgca gaccugguua       60

gaaucaagag aauacacgaa gcacuugauc aagguugaaa acuggauauu caggaacccc      120

ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg gaagcucgac gagccaaaaa      180

gucauauacu uggucaugau acugcugauu gccccggcau acagu                      225


<210>  93
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prM CDS mRNA wild type

<400>  93
gcagagauca cuagacgcgg gagugcauac uacauguacu uggauaggag cgaugccggg       60

aaggccauuu cguuugcuac cacauuggga gugaacaagu gccacguaca gaucauggac      120

cucgggcaca ugugugacgc caccaugagu uaugagugcc cuaugcugga ugagggagug      180

gaaccagaug augucgauug cuggugcaac acgacaucaa cuuggguugu guacggaacc      240

ugucaucaca aaaaagguga ggcacggcga ucuagaagag ccgugacgcu cccuucucac      300

ucuacaagga aguugcaaac gcggucgcag accugguuag aaucaagaga auacacgaag      360

cacuugauca agguugaaaa cuggauauuc aggaaccccg gguuugcgcu aguggccguu      420

gccauugccu ggcuuuuggg aagcucgacg agccaaaaag ucauauacuu ggucaugaua      480

cugcugauug ccccggcaua cagu                                             504


<210>  94
<211>  1500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) E CDS mRNA wild type

<400>  94
aucaggugca uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg       60

guugauguug ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca      120

gucgacauag aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc      180

uacgaggcau cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc      240

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu      300

uggggaaacg guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg      360

uguucuaaga agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug      420

cuaucagugc auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga      480

gcgaaagucg agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga      540

agcuuaggac uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug      600

accaugaaca auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu      660

uggcaugcug gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa      720

uucaaggaug cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc      780

guucacacgg cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug      840

uucucuggcc auuugaaaug ccgccuaaaa auggacaagc uuagauugaa gggcguguca      900

uauuccuugu gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga      960

acagucacag uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag     1020

auggcggugg acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug     1080

auuacugaaa gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau     1140

ucuuacauug ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu     1200

agcaccaucg gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug     1260

ggggauacag ccugggacuu cggaucaguc gggggugugu ucaacucacu ggguaagggc     1320

auucaccaga uuuuuggagc agccuucaaa ucacuguuug gaggaauguc cugguucuca     1380

cagauccuca uaggcacgcu gcuagugugg uuagguuuga acacaaagaa uggaucuauc     1440

ucccucacau gcuuggcccu ggggggagug augaucuucc ucuccacggc uguuucugcu     1500


<210>  95
<211>  2004
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME CDS mRNA wild type

<400>  95
gcagagauca cuagacgcgg gagugcauac uacauguacu uggauaggag cgaugccggg       60

aaggccauuu cguuugcuac cacauuggga gugaacaagu gccacguaca gaucauggac      120

cucgggcaca ugugugacgc caccaugagu uaugagugcc cuaugcugga ugagggagug      180

gaaccagaug augucgauug cuggugcaac acgacaucaa cuuggguugu guacggaacc      240

ugucaucaca aaaaagguga ggcacggcga ucuagaagag ccgugacgcu cccuucucac      300

ucuacaagga aguugcaaac gcggucgcag accugguuag aaucaagaga auacacgaag      360

cacuugauca agguugaaaa cuggauauuc aggaaccccg gguuugcgcu aguggccguu      420

gccauugccu ggcuuuuggg aagcucgacg agccaaaaag ucauauacuu ggucaugaua      480

cugcugauug ccccggcaua caguaucagg ugcauuggag ucagcaauag agacuucgug      540

gagggcaugu caggugggac cuggguugau guugucuugg aacauggagg cugcguuacc      600

gugauggcac aggacaagcc aacagucgac auagaguugg ucacgacgac gguuaguaac      660

auggccgagg uaagauccua uugcuacgag gcaucgauau cggacauggc uucggacagu      720

cguugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      780

aaaagaacau uaguggacag agguugggga aacgguugug gacuuuuugg caaagggagc      840

uuggugacau gugccaaguu uacguguucu aagaagauga ccgggaagag cauucaaccg      900

gaaaaucugg aguaucggau aaugcuauca gugcauggcu cccagcauag cgggaugauu      960

ggauaugaaa cugacgaaga uagagcgaaa gucgagguua cgccuaauuc accaagagcg     1020

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu     1080

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1140

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg     1200

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1260

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1320

auggauggug caaagggaag gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1380

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1440

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1500

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1560

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1620

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1680

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1740

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1800

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1860

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     1920

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     1980

uuccucucca cggcuguuuc ugcu                                            2004


<210>  96
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS1 CDS mRNA wild type

<400>  96
gacguggggu gcucagugga cuucucaaaa aaggaaacga gauguggcac ggggguauuc       60

aucuauaaug auguugaagc cuggagggac cgguacaagu accauccuga cuccccccgc      120

agauuggcag cagcagucaa gcaggccugg gaagagggga ucugugggau cucauccguu      180

ucaagaaugg aaaacaucau guggaaauca guagaagggg agcucaaugc uauccuagag      240

gagaauggag uucaacugac aguuguugug ggaucuguaa aaaaccccau guggagaggu      300

ccacaaagau ugccagugcc ugugaaugag cugccccaug gcuggaaagc cugggggaaa      360

ucguauuuug uuagggcggc aaagaccaac aacaguuuug uugucgacgg ugacacacug      420

aaggaauguc cgcuugagca cagagcaugg aauaguuuuc uuguggagga ucacggguuu      480

ggagucuucc acaccagugu cuggcuuaag gucagagaag auuacucauu agaaugugac      540

ccagccguca uaggaacagc uguuaaggga agggaggccg cgcacaguga ucugggcuau      600

uggauugaaa gugaaaagaa ugacacaugg aggcugaaga gggcccaccu gauugagaug      660

aaaacaugug aauggccaaa gucucacaca uuguggacag auggaguaga agaaagugau      720

cuuaucauac ccaagucuuu agcuggucca cucagccacc acaacaccag agaggguuac      780

agaacccaag ugaaagggcc auggcacagu gaagagcuug aaauccgguu ugaggaaugu      840

ccaggcacca agguuuacgu ggaggagaca ugcggaacua gaggaccauc ucugagauca      900

acuacugcaa guggaagggu cauugaggaa uggugcugua gggaaugcac aaugccccca      960

cuaucguuuc gagcaaaaga cggcugcugg uauggaaugg agauaaggcc caggaaagaa     1020

ccagagagca acuuagugag gucaauggug acagcg                               1056


<210>  97
<211>  678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS2A CDS mRNA wild type

<400>  97
gggucaaccg aucauaugga ccacuucucu cuuggagugc uugugauucu acucauggug       60

caggaggggu ugaagaagag aaugaccaca aagaucauca ugagcacauc aauggcagug      120

cugguaguca ugaucuuggg aggauuuuca augagugacc uggccaagcu ugugauccug      180

augggugcua cuuucgcaga aaugaacacu ggaggagaug uagcucacuu ggcauuggua      240

gcggcauuua aagucagacc agccuugcug gucuccuuca uuuucagagc caauuggaca      300

ccccgugaga gcaugcugcu agcccuggcu ucgugucuuc ugcaaacugc gaucucugcu      360

cuugaaggug acuugauggu ccucauuaau ggauuugcuu uggccugguu ggcaauucga      420

gcaauggccg ugccacgcac ugacaacauc gcucuaccaa ucuuggcugc ucuaacacca      480

cuagcucgag gcacacugcu cguggcaugg agagcgggcc uggcuacuug uggagggauc      540

augcuccucu cccugaaagg gaaagguagu gugaagaaga accugccauu ugucauggcc      600

cugggauuga cagcugugag gguaguagac ccuauuaaug ugguaggacu acuguuacuc      660

acaaggagug ggaagcgg                                                    678


<210>  98
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS2B CDS mRNA wild type

<400>  98
agcuggcccc cuagugaagu ucucacagcc guuggccuga uaugugcacu ggccggaggg       60

uuugccaagg cagacauuga gauggcugga cccauggcug caguaggcuu gcuaauuguc      120

agcuaugugg ucucgggaaa gaguguggac auguacauug aaagagcagg ugacaucaca      180

ugggaaaagg acgcggaagu cacuggaaac aguccucggc uugacguggc acuggaugag      240

aguggugacu ucuccuuggu agaggaagau gguccaccca ugagagagau cauacucaag      300

gugguccuga uggccaucug uggcaugaac ccaauagcua uaccuuuugc ugcaggagcg      360

ugguaugugu augugaagac ugggaaaagg                                       390


<210>  99
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS3 CDS mRNA wild type

<400>  99
aguggcgccc ucugggacgu gccugcuccc aaagaaguga agaaaggaga gaccacagau       60

ggaguguaca gagugaugac ucgcagacug cuagguucaa cacagguugg agugggaguc      120

augcaagagg gagucuucca caccaugugg cacguuacaa aaggagccgc acugaggagc      180

ggugagggaa gacuugaucc auacuggggg gaugucaagc aggacuuggu gucauacugu      240

gggccuugga aguuggaugc agcuugggau ggacucagcg agguacagcu uuuggccgua      300

ccucccggag agagggccag aaacauucag acccugccug gaauauucaa gacaaaggac      360

ggggacaucg gagcaguugc ucuggacuac ccugcaggga ccucaggauc uccgauccua      420

gacaaaugug gaagagugau aggacucuau ggcaaugggg uugugaucaa gaauggaagc      480

uauguuagug cuauaaccca gggaaagagg gaggaggaga cuccgguuga auguuucgaa      540

cccucgaugc ugaagaagaa gcagcuaacu gucuuggauc ugcauccagg agccggaaaa      600

accaggagag uucuuccuga aauaguccgu gaagccauaa aaaagagacu ccggacagug      660

aucuuggcac caacuagggu ugucgcugcu gagauggagg aggccuugag aggacuuccg      720

gugcguuaca ugacaacagc agucaacguc acccauucug ggacagaaau cguugauuug      780

augugccaug ccacuuucac uucacgcuua cuacaaccca ucagaguccc uaauuacaau      840

cucaacauca uggaugaagc ccacuucaca gaccccucaa guauagcugc aagaggauac      900

auaucaacaa ggguugaaau gggcgaggcg gcugccauuu uuaugacugc cacaccacca      960

ggaacccgug augcguuucc ugacucuaac ucaccaauca uggacacaga aguggaaguc     1020

ccagagagag ccuggagcuc aggcuuugau ugggugacag accauucugg gaaaacaguu     1080

ugguucguuc caagcgugag aaacggaaau gaaaucgcag ccugucugac aaaggcugga     1140

aagcggguca uacagcucag caggaagacu uuugagacag aauuucagaa aacaaaaaau     1200

caagaguggg acuuugucau aacaacugac aucucagaga ugggcgccaa cuucaaggcu     1260

gaccggguca uagacucuag gagaugccua aaaccaguca uacuugaugg ugagagaguc     1320

aucuuggcug ggcccaugcc ugucacgcau gcuagugcug cucagaggag aggacguaua     1380

ggcaggaacc cuaacaaacc uggagaugag uacauguaug gaggugggug ugcagagacu     1440

gaugaaggcc augcacacug gcuugaagca agaaugcuuc uugacaacau cuaccuccag     1500

gauggccuca uagccucgcu cuaucggccu gaggccgaua agguagccgc cauugaggga     1560

gaguuuaagc ugaggacaga gcaaaggaag accuucgugg aacucaugaa gagaggagac     1620

cuucccgucu ggcuagccua ucagguugca ucugccggaa uaacuuacac agacagaaga     1680

uggugcuuug auggcacaac caacaacacc auaauggaag acaguguacc agcagagguu     1740

uggacaaagu auggagagaa gagagugcuc aaaccgagau ggauggaugc uagggucugu     1800

ucagaccaug cggcccugaa gucguucaaa gaauucgccg cuggaaaaag a              1851


<210>  100
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS4A CDS mRNA wild type

<400>  100
ggagcggcuu ugggaguaau ggaggcccug ggaacacugc caggacacau gacagagagg       60

uuucaggaag ccauugacaa ccucgccgug cucaugcgag cagagacugg aagcaggccu      120

uauaaggcag cggcagccca acugccggag acccuagaga ccauuaugcu cuuagguuug      180

cugggaacag uuucacuggg gaucuucuuc gucuugaugc ggaauaaggg caucgggaag      240

augggcuuug gaaugguaac ccuuggggcc agugcauggc ucauguggcu uucggaaauu      300

gaaccagcca gaauugcaug uguccucauu guuguguuuu uauuacuggu ggugcucaua      360

cccgagccag agaagcaaag aucuccccaa gauaaccaga uggcaauuau caucauggug      420

gcagugggcc uucuagguuu gauaacugca                                       450


<210>  101
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS4B CDS mRNA wild type

<400>  101
aacgaacuug gauggcugga aagaacaaaa aaugacauag cucaucuaau gggaaggaga       60

gaagaaggag caaccauggg auucucaaug gacauugauc ugcggccagc cuccgccugg      120

gcuaucuaug ccgcauugac aacucucauc accccagcug uccaacaugc gguaaccacu      180

ucauacaaca acuacuccuu aauggcgaug gccacacaag cuggagugcu guuuggcaug      240

ggcaaaggga ugccauuuau gcauggggac cuuggagucc cgcugcuaau gauggguugc      300

uauucacaau uaacaccccu gacucugaua guagcuauca uucugcuugu ggcgcacuac      360

auguacuuga ucccaggccu acaagcggca gcagcgcgug cugcccagaa aaggacagca      420

gcuggcauca ugaagaaucc cguuguggau ggaauagugg uaacugacau ugacacaaug      480

acaauagacc cccaggugga gaagaagaug ggacaagugu uacucauagc aguagccauc      540

uccagugcug ugcugcugcg gaccgccugg ggaugggggg aggcuggagc ucugaucaca      600

gcagcgaccu ccaccuugug ggaaggcucu ccaaacaaau acuggaacuc cucuacagcc      660

accucacugu gcaacaucuu cagaggaagc uaucuggcag gagcuucccu uaucuauaca      720

gugacgagaa acgcuggccu gguuaagaga cgu                                   753


<210>  102
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS5 CDS mRNA wild type

<400>  102
ggagguggga cgggagagac ucugggagag aaguggaaag cucgucugaa ucagaugucg       60

gcccuggagu ucuacucuua uaaaaaguca gguaucacug aaguguguag agaggaggcu      120

cgccgugccc ucaaggaugg aguggccaca ggaggacaug ccguaucccg gggaagugca      180

aagaucagau gguuggagga gagaggauau cugcagcccu augggaaggu uguugaccuc      240

ggauguggca gagggggcug gagcuauuau gccgccacca uccgcaaagu gcaggaggug      300

agaggauaca caaagggagg ucccggucau gaagaaccca ugcuggugca aagcuauggg      360

uggaacauag uucgucucaa gaguggagug gacgucuucc acauggcggc ugagccgugu      420

gacacucugc ugugugacau aggugaguca ucaucuaguc cugaagugga agagacacga      480

acacucagag ugcucucuau ggugggggac uggcuugaaa aaagaccagg ggccuucugu      540

auaaaggugc ugugcccaua caccagcacu augauggaaa ccauggagcg acugcaacgu      600

aggcaugggg gaggauuagu cagagugcca uugugucgca acuccacaca ugagauguac      660

ugggucucug gggcaaagag caacaucaua aaaagugugu ccaccacaag ucagcuccuc      720

cugggacgca uggauggccc caggaggcca gugaaauaug aggaggaugu gaaccucggc      780

ucggguacac gagcuguggc aagcugugcu gaggcuccua acaugaaaau caucggcagg      840

cgcauugaga gaauccgcaa ugaacaugca gaaacauggu uucuugauga aaaccaccca      900

uacaggacau gggccuacca ugggagcuac gaagccccca cgcaaggauc agcgucuucc      960

cucgugaacg ggguuguuag acuccuguca aagccuuggg acguggugac uggaguuaca     1020

ggaauagcca ugacugacac cacaccauac ggccaacaaa gagucuucaa agaaaaagug     1080

gacaccaggg ugccagaucc ccaagaaggc acucgccagg uaaugaacau agucucuucc     1140

uggcugugga aggagcuggg gaaacgcaag cggccacgcg ucugcaccaa agaagaguuu     1200

aucaacaagg ugcgcagcaa ugcagcacug ggagcaauau uugaagagga aaaagaaugg     1260

aagacggcug uggaagcugu gaaugaucca agguuuuggg cccuagugga uagggagaga     1320

gaacaccacc ugagaggaga gugucacagc uguguguaca acaugauggg aaaaagagaa     1380

aagaagcaag gagaguucgg gaaagcaaaa gguagccgcg ccaucuggua caugugguug     1440

ggagccagau ucuuggaguu ugaagcccuu ggauucuuga acgaggacca uuggauggga     1500

agagaaaacu caggaggugg agucgaaggg uuaggauugc aaagacuugg auacauucua     1560

gaagaaauga aucgggcacc aggaggaaag auguacgcag augacacugc uggcugggac     1620

acccgcauua guaaguuuga ucuggagaau gaagcucuga uuaccaacca aauggaggaa     1680

gggcacagaa cucuggcguu ggccgugauu aaauacacau accaaaacaa aguggugaag     1740

guucucagac cagcugaagg aggaaaaaca guuauggaca ucauuucaag acaagaccag     1800

agagggagug gacaaguugu cacuuaugcu cucaacacau ucaccaacuu gguggugcag     1860

cuuauccgga acauggaagc ugaggaagug uuagagaugc aagacuuaug guuguugagg     1920

aagccagaga aagugaccag augguugcag agcaauggau gggauagacu caaacgaaug     1980

gcggucagug gagaugacug cguugugaag ccaaucgaug auagguuugc acaugcccuc     2040

agguucuuga augacauggg aaaaguuagg aaagacacac aggaguggaa acccucgacu     2100

ggauggagca auugggaaga agucccguuc ugcucccacc acuucaacaa gcuguaccuc     2160

aaggauggga gauccauugu ggucccuugc cgccaccaag augaacugau uggccgagcu     2220

cgcgucucac caggggcagg auggagcauc cgggagacug ccugucuugc aaaaucauau     2280

gcgcagaugu ggcagcuccu uuauuuccac agaagagacc uucgacugau ggcuaaugcc     2340

auuugcucgg cugugccagu ugacugggua ccaacuggga gaaccaccug gucaauccau     2400

ggaaagggag aauggaugac cacugaggac augcucaugg uguggaauag aguguggauu     2460

gaggagaacg accauaugga ggacaagacu ccuguaacaa aauggacaga cauucccuau     2520

cuaggaaaaa gggaggacuu auggugugga ucccuuauag ggcacagacc ccgcaccacu     2580

ugggcugaaa acaucaaaga cacagucaac auggugcgca ggaucauagg ugaugaagaa     2640

aaguacaugg acuaucuauc cacccaaguc cgcuacuugg gugaggaagg guccacaccc     2700

ggaguguug                                                             2709


<210>  103
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS mRNA wild type

<400>  103
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa     1080

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaagcuua     1140

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug     1200

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauucaag     1320

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac     1380

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu     1440

ggccauuuga aaugccgccu aaaaauggac aagcuuagau ugaagggcgu gucauauucc     1500

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1560

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1620

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1680

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1740

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1800

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1860

acagccuggg acuucggauc agucgggggu guguucaacu cacuggguaa gggcauucac     1920

cagauuuuug gagcagccuu caaaucacug uuuggaggaa uguccugguu cucacagauc     1980

cucauaggca cgcugcuagu gugguuaggu uugaacacaa agaauggauc uaucucccuc     2040

acaugcuugg cccugggggg agugaugauc uuccucucca cggcuguuuc ugcugacgug     2100

gggugcucag uggacuucuc aaaa                                            2124


<210>  104
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS mRNA wild type

<400>  104
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa     1080

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaaguuua     1140

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug     1200

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauuuaag     1320

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac     1380

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu     1440

ggccauuuga aaugccgccu aaaaauggac aagcucagau ugaagggcgu gucauauucc     1500

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1560

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1620

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1680

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1740

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1800

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1860

acagccuggg acuucggauc agucgggggu guguucaacu cacuggguaa gggcauucac     1920

cagauuuuug gagcagccuu caaaucacug uuuggaggaa uguccugguu cucacagauc     1980

cucauaggca cgcugcuagu gugguuaggu uugaacacaa agaauggauc uaucucccuc     2040

acaugcuugg cccugggggg agugaugauc uuccucucca cggcuguuuc ugcugacgug     2100

gggugcucag uggacuucuc aaaa                                            2124


<210>  105
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS mRNA wild type

<400>  105
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      180

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      360

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      420

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca      480

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa      540

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaagcuua      600

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug      660

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau      720

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauucaag      780

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac      840

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu      900

ggccauuuga aaugccgccu aaaaauggac aagcuuagau ugaagggcgu gucauauucc      960

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1020

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1080

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1140

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1200

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1260

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1320

acagccuggg acuucggauc a                                               1341


<210>  106
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS mRNA wild type

<400>  106
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      180

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      360

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      420

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca      480

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa      540

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaaguuua      600

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug      660

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau      720

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauuuaag      780

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac      840

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu      900

ggccauuuga aaugccgccu aaaaauggac aagcucagau ugaagggcgu gucauauucc      960

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1020

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1080

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1140

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1200

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1260

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1320

acagccuggg acuucggauc a                                               1341


<210>  107
<211>  10269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein CDS mRNA GC

<400>  107
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc      360

auggcggccg aggucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      420

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      480

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      600

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      660

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      720

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      780

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      840

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      900

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      960

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug     1020

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc     1080

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac     1140

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc     1260

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1320

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1380

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1440

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1560

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1620

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1680

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1740

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1860

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1920

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1980

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     2040

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     2100

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     2160

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     2220

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     2280

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2340

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcuccguc     2400

gacuucagca agaaggagac ccgcugcggg acgggcgugu ucguguacaa cgacgucgag     2460

gccuggcggg accgcuacaa guaccacccc gacuccccgc gccggcuggc cgccgcggug     2520

aagcaggccu gggaggacgg gaucugcggc aucagcuccg ugagccgcau ggagaacauc     2580

auguggcgga gcgucgaggg ggagcugaac gccauccucg aggagaacgg cgugcagcug     2640

accguggucg ugggcuccgu gaagaacccg auguggcgcg ggccccagcg gcugcccguc     2700

ccggugaacg agcuccccca cggcuggaag gccuggggga agagccacuu cgugcgcgcg     2760

gccaagacca acaacuccuu cgucguggac ggcgacaccc ugaaggagug cccccugaag     2820

caccgggccu ggaacagcuu ccucguggag gaccacgggu ucggcgucuu ccacacgucc     2880

guguggcuga aggugcgcga ggacuacagc cuggagugcg accccgccgu caucggcacc     2940

gccgugaagg ggaaggaggc ggugcacucc gaccucggcu acuggaucga gagcgagaag     3000

aacgacaccu ggcggcugaa gcgcgcccac cugaucgaga ugaagaccug cgaguggccg     3060

aagucccaca cgcucuggac cgacgggauc gaggagagcg accugaucau ccccaagucc     3120

cuggccggcc cccucagcca ccacaacacc cgggaggggu accgcaccca gaugaagggc     3180

cccuggcacu ccgaggagcu ggagauccgg uucgaggagu gcccgggcac gaagguccac     3240

guggaggaga ccugcgggac ccgcggcccc agccugcggu ccaccacggc cagcgggcgc     3300

gucaucgagg aguggugcug ccgggagugc accaugcccc cgcucuccuu ccgcgccaag     3360

gacggcugcu gguacggcau ggagauccgg ccgcgcaagg agcccgagag caaccuggug     3420

cgguccaugg ugaccgcggg gagcaccgac cacauggacc acuucagccu gggcguccuc     3480

gugauccugc ugauggugca ggaggggcuc aagaagcgca ugacgaccaa gaucaucauc     3540

uccaccagca uggccguccu gguggccaug auccugggcg gguucuccau gagcgaccuc     3600

gccaagcugg ccauccugau gggcgcgacc uucgccgaga ugaacaccgg cggggacgug     3660

gcccaccucg cccugaucgc cgcguucaag guccgccccg cccugcucgu guccuucauc     3720

uuccgggcca acuggacgcc ccgcgagagc augcugcugg cccucgcguc cugccugcug     3780

cagaccgcca ucagcgcccu cgagggcgac cugauggugc ugaucaacgg guucgcccuc     3840

gccuggcugg cgauccgggc cauggucgug ccgcgcaccg acaacaucac ccuggccauc     3900

cucgccgccc ugacgccccu ggcgcggggc acccuccugg uggccuggcg cgccgggcug     3960

gccacgugcg gcggcuucau gcuccugucc cugaagggga agggcagcgu caagaagaac     4020

cuccccuucg ugauggcccu ggggcugacc gcggugcggc ucgucgaccc caucaacgug     4080

gugggccugc ugcuccugac gcgcuccggg aagcggagcu ggccgcccuc cgagguccug     4140

accgccgugg gccucaucug cgcccuggcc ggcggguucg cgaaggccga caucgagaug     4200

gccggcccca uggccgccgu ggggcugcuc aucgucagcu acgugguguc cggcaagagc     4260

gucgacaugu acaucgagcg cgcgggggac aucaccuggg agaaggacgc cgaggugacc     4320

ggcaacuccc cgcggcugga cguggcccug gacgagagcg gcgacuucuc ccucgucgag     4380

gacgacgggc cgcccaugcg cgagaucauc cugaaggugg ugcugaugac gaucugcggc     4440

augaacccca ucgccauccc guucgccgcg ggggccuggu acgucuacgu gaagaccggc     4500

aagcggagcg gcgcccugug ggacgugccc gcccccaagg aggucaagaa gggggagacc     4560

accgacggcg uguaccgcgu gaugacgcgg cgccuccugg ggagcaccca ggucggcgug     4620

ggggugaugc aggagggcgu cuuccacacc auguggcacg ugaccaaggg cuccgcccug     4680

cggagcgggg agggccgccu cgaccccuac uggggcgacg ugaagcagga ccuggucucc     4740

uacugcgggc cguggaagcu ggacgcggcc ugggacgggc acagcgaggu gcagcuccug     4800

gccgugcccc cgggcgagcg ggcccgcaac auccagacgc ugcccggcau cuucaagacc     4860

aaggacgggg acaucggcgc ggucgcccuc gacuacccgg ccgggaccuc cggcagcccc     4920

auccuggaca agugcgggcg cgugaucggc cuguacggca acgggguggu caucaagaac     4980

ggcuccuacg ugagcgccau cacccagggg cggcgcgagg aggagacgcc cgucgagugc     5040

uucgagcccu ccaugcucaa gaagaagcag cugaccgugc uggaccucca cccgggcgcc     5100

gggaagaccc ggcgcgugcu gcccgagauc guccgggagg cgaucaagac ccgccugcgg     5160

acggugaucc ucgcccccac ccgcgugguc gccgccgaga uggaggaggc ccugcggggc     5220

cugcccgugc gcuacaugac caccgcggug aacgucacgc acagcggcac cgagaucgug     5280

gaccucaugu gccacgccac cuucaccucc cggcugcugc agccgauccg cgugcccaac     5340

uacaaccucu acaucaugga cgaggcccac uucacggacc ccagcuccau cgccgcccgg     5400

ggguacauca gcacccgcgu cgagaugggc gaggcggccg ccaucuucau gaccgccacc     5460

ccgccgggga cccgggacgc cuuccccgac uccaacagcc ccaucaugga cacggaggug     5520

gaggugcccg agcgcgcgug guccagcggc uucgacuggg ucaccgacua cuccggcaag     5580

accguguggu ucgugccgag cguccggaac gggaacgaga ucgccgccug ccugaccaag     5640

gccggcaagc gcgugaucca gcugagccgg aagacguucg agaccgaguu ccagaagacc     5700

aagcaccagg agugggacuu cguggucacc acggacaucu ccgagauggg ggcgaacuuc     5760

aaggccgacc gcgugaucga cagccggcgc ugccucaagc ccgugauccu ggacggcgag     5820

cgcgucaucc uggccgggcc caugcccgug acccacgccu ccgccgcgca gcggcgcggc     5880

cggaucggcc gcaacccgaa caagcccggg gacgaguacc ucuacggcgg gggcugcgcc     5940

gagaccgacg aggaccacgc ccacuggcug gaggcccgga ugcugcucga caacaucuac     6000

cugcaggacg ggcugaucgc cagccucuac cgccccgagg cggacaaggu ggccgccauc     6060

gagggcgagu ucaagcugcg gaccgagcag cgcaagacgu ucgucgagcu gaugaagcgg     6120

ggcgaccucc cgguguggcu ggccuaccag guggccuccg cggggaucac cuacaccgac     6180

cgccgguggu gcuucgacgg caccacgaac aacaccauca uggaggacag cguccccgcc     6240

gaggugugga cccgccacgg ggagaagcgg gugcugaagc cccgcuggau ggacgcccgg     6300

gucugcuccg accacgccgc gcucaagagc uucaaggagu ucgccgccgg caagcgcggg     6360

gccgccuucg gcgugaugga ggcgcugggc acccugcccg ggcacaugac ggagcgguuc     6420

caggaggcca ucgacaaccu cgccgugcug augcgcgccg agaccggcuc ccggccguac     6480

aaggccgcgg ccgcccagcu gcccgagacc cucgagacca ucaugcugcu ggggcuccug     6540

ggcacgguca gccugggcau cuucuucgug cucaugcgca acaaggggau cggcaagaug     6600

ggguucggca uggugacccu gggggccucc gccuggcuga uguggcucag cgagaucgag     6660

cccgcgcgca ucgccugcgu ccugaucgug guguuccugc uccuggucgu gcugaucccc     6720

gagccggaga agcagcgguc cccgcaggac aaccagaugg ccaucaucau caugguggcc     6780

gucggccucc ugggccugau caccgcgaac gagcucgggu ggcuggagcg caccaagagc     6840

gaccuguccc accucauggg ccggcgcgag gagggggcca cgaugggcuu cagcauggac     6900

aucgaccugc ggcccgccuc cgccugggcc aucuacgcgg cccugaccac cuucaucacc     6960

cccgccgugc agcacgccgu gacgaccagc uacaacaacu acucccucau ggccauggcg     7020

acccaggccg ggguccuguu cggcaugggc aaggggaugc cguucuacgc cugggacuuc     7080

ggcgugccgc ugcucaugau cgggugcuac agccagcuga ccccgcugac gcucaucgug     7140

gccaucaucc ugcuggucgc ccacuacaug uaccucaucc ccggccugca ggcggccgcc     7200

gcccgcgccg cgcagaagcg gaccgccgcc gggaucauga agaacccggu ggucgacggc     7260

aucgugguga ccgacaucga caccaugacg aucgaccccc aggucgagaa gaagaugggc     7320

caggugcugc ucauggccgu ggcggucagc uccgccaucc ugagccgcac cgccuggggg     7380

uggggcgagg ccggggcccu gaucaccgcg gccaccucca cccucuggga gggcagcccc     7440

aacaaguacu ggaacuccag cacggccacc ucccugugca acaucuuccg ggggagcuac     7500

cuggccggcg ccucccucau cuacaccgug acccgcaacg cgggccuggu gaagcggcgc     7560

gggggcggga cgggcgagac ccugggcgag aaguggaagg cccggcucaa ccagaugagc     7620

gcccuggagu ucuacuccua caagaagagc gggaucaccg aggucugccg cgaggaggcc     7680

cggcgcgccc ugaaggacgg cguggcgacc gggggccacg ccgugucccg ggggagcgcc     7740

aagcuccgcu ggcuggucga gcggggcuac cugcagcccu acggcaaggu gaucgaccug     7800

gggugcggcc gcgggggcug guccuacuac gccgcgacga uccggaaggu gcaggagguc     7860

aagggguaca ccaagggcgg cccggggcac gaggagcccg ugcucgugca gagcuacggc     7920

uggaacaucg uccgccugaa guccggggug gacguguucc acauggccgc cgagcccugc     7980

gacacccugc ucugcgacau cggcgagagc uccagcagcc ccgaggucga ggaggcccgc     8040

acccugcggg ugcuguccau ggugggggac uggcucgaga agcgcccggg cgccuucugc     8100

aucaaggucc ugugccccua cacgagcacc augauggaga cccuggagcg gcuccagcgc     8160

cgguacggcg ggggccuggu gcgcgugccc cugucccgga acagcaccca cgagauguac     8220

ugggucuccg gggcgaagag caacacgauc aaguccguga gcaccaccuc ccagcuccug     8280

cugggccgca uggacggccc ccggcgcccg gugaaguacg aggaggacgu caaccucggg     8340

agcggcaccc gggccguggu guccugcgcc gaggccccca acaugaagau caucgggaac     8400

cgcaucgagc ggauccgcag cgagcacgcc gagacguggu ucuucgacga gaaccacccc     8460

uaccggaccu gggcguacca cggcuccuac gaggccccga cccaggggag cgccuccagc     8520

cugaucaacg gcgucgugcg ccugcucucc aagcccuggg acguggucac cggcgugacg     8580

gggaucgcca ugaccgacac cacccccuac ggccagcagc ggguguucaa ggagaagguc     8640

gacacgcgcg ugcccgaccc gcaggagggg acccgccagg ugaugagcau ggucagcucc     8700

uggcugugga aggagcuggg caagcacaag cgcccccgcg ugugcaccaa ggaggaguuc     8760

aucaacaagg ugcggagcaa cgccgcgcuc ggggccaucu ucgaggagga gaaggagugg     8820

aagaccgccg ucgaggccgu gaacgacccc cgcuucuggg cccuggugga caaggagcgg     8880

gagcaccacc uccgcggcga gugccagucc ugcgucuaca acaugauggg caagcgggag     8940

aagaagcagg gggaguucgg caaggcgaag gggagccgcg ccaucuggua cauguggcuc     9000

ggcgcccggu uccuggaguu cgaggcccug ggguuccuca acgaggacca cuggaugggc     9060

cgcgagaacu ccggcggggg cguggagggg cugggccugc agcggcucgg guacgugcug     9120

gaggagauga gccgcauccc cggcggccgg auguacgcgg acgacacggc cgggugggac     9180

acccgcaucu cccgguucga ccuggagaac gaggcccuca ucaccaacca gauggagaag     9240

ggccaccgcg cccuggcccu ggcgaucauc aaguacaccu accagaacaa ggucgugaag     9300

guccuccggc cggccgagaa ggggaagacg gugauggaca ucaucagccg ccaggaccag     9360

cggggcuccg gccagguggu caccuacgcc cugaacaccu ucaccaaccu gguggugcag     9420

cucauccgca acauggaggc cgaggagguc cuggagaugc aggaccugug gcuccugcgg     9480

cgcagcgaga aggugaccaa cuggcugcag uccaacgggu gggaccggcu caagcgcaug     9540

gccgugagcg gcgacgacug cgucgugaag cccaucgacg accgcuucgc gcacgcccug     9600

cgguuccuga acgacauggg gaaggugcgc aaggacacgc aggaguggaa gcccuccacc     9660

ggcugggaca acugggagga gguccccuuc ugcagccacc acuucaacaa gcuccaccug     9720

aaggacgggc gguccaucgu ggugccgugc cgccaccagg acgagcugau cggccgggcc     9780

cgcgucagcc ccggcgccgg gugguccauc cgggagaccg ccugccucgc gaagagcuac     9840

gcccagaugu ggcagcugcu guacuuccac cgccgggacc uccgccugau ggccaacgcc     9900

aucugcucca gcgugcccgu ggacuggguc cccaccggcc ggacgaccug gagcauccac     9960

gggaagggcg aguggaugac caccgaggac augcuggugg uguggaaccg cgucuggauc    10020

gaggagaacg accacaugga ggacaagacg ccggugacca aguggaccga cauccccuac    10080

cucgggaagc gggaggaccu guggugcggc ucccugaucg gccaccgccc ccggaccacg    10140

ugggcggaga acaucaagaa caccgugaac augguccgcc ggaucaucgg ggacgaggag    10200

aaguacaugg acuaccucag cacccaggug cgcuaccugg gcgaggaggg guccaccccc    10260

ggcgugcug                                                            10269


<210>  108
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C CDS mRNA GC

<400>  108
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gc                                                          312


<210>  109
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-ER anchor CDS mRNA GC

<400>  109
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc      360

auggcg                                                                 366


<210>  110
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) pr CDS mRNA GC

<400>  110
gccgagguca cccggcgcgg cuccgccuac uacauguacc ucgaccggaa cgacgccggg       60

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      120

cuggggcaca ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg      240

ugccaccaca agaaggggga ggcccgccgg ucccgccgg                             279


<210>  111
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) M CDS mRNA GC

<400>  111
gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc       60

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      120

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      180

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acucc                      225


<210>  112
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prM CDS mRNA GC

<400>  112
gccgagguca cccggcgcgg cuccgccuac uacauguacc ucgaccggaa cgacgccggg       60

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      120

cuggggcaca ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg      240

ugccaccaca agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac      300

uccacccgca agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag      360

caccugaucc gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc      420

gccaucgccu ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc      480

cuccugaucg cccccgcgua cucc                                             504


<210>  113
<211>  1512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) E CDS mRNA GC

<400>  113
auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg       60

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      120

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      180

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      240

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      300

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      360

ugcuccaaga agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug      420

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg      480

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug      540

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac      600

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      660

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag      720

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc      780

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc      840

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug      900

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag      960

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1020

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1080

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1140

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1200

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1260

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1320

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1380

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     1440

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     1500

gcggugagcg cc                                                         1512


<210>  114
<211>  2016
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME CDS mRNA GC

<400>  114
gccgagguca cccggcgcgg cuccgccuac uacauguacc ucgaccggaa cgacgccggg       60

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      120

cuggggcaca ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg      240

ugccaccaca agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac      300

uccacccgca agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag      360

caccugaucc gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc      420

gccaucgccu ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc      480

cuccugaucg cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug      540

gaggggaugu ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc      600

gugauggccc aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac      660

auggccgagg uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc      720

cggugcccca cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc      780

aagcgcacgc uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc      840

cuggugaccu gcgccaaguu cgccugcucc aagaagauga ccgggaagag cauccagccg      900

gagaaccugg aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc      960

gucaacgaca ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac     1020

agcccccgcg cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc     1080

cggaccggcc uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug     1140

gugcacaagg agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg     1200

accccgcacu ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc     1260

cagaccgugg ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg     1320

cuggaggccg agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc     1380

cugaagaugg acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc     1440

accuucacca agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac     1500

gccgggaccg acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug     1560

acgcccgugg ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc     1620

aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg     1680

gagaagaaga ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag     1740

gccaccgucc gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc     1800

agcgugggcg gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc     1860

uucaagagcc uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug     1920

auguggcucg ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc     1980

ggcguccuga ucuuccucuc caccgcggug agcgcc                               2016


<210>  115
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS1 CDS mRNA GC

<400>  115
gacguggggu gcuccgucga cuucagcaag aaggagaccc gcugcgggac gggcguguuc       60

guguacaacg acgucgaggc cuggcgggac cgcuacaagu accaccccga cuccccgcgc      120

cggcuggccg ccgcggugaa gcaggccugg gaggacggga ucugcggcau cagcuccgug      180

agccgcaugg agaacaucau guggcggagc gucgaggggg agcugaacgc cauccucgag      240

gagaacggcg ugcagcugac cguggucgug ggcuccguga agaacccgau guggcgcggg      300

ccccagcggc ugcccguccc ggugaacgag cucccccacg gcuggaaggc cugggggaag      360

agccacuucg ugcgcgcggc caagaccaac aacuccuucg ucguggacgg cgacacccug      420

aaggagugcc cccugaagca ccgggccugg aacagcuucc ucguggagga ccacggguuc      480

ggcgucuucc acacguccgu guggcugaag gugcgcgagg acuacagccu ggagugcgac      540

cccgccguca ucggcaccgc cgugaagggg aaggaggcgg ugcacuccga ccucggcuac      600

uggaucgaga gcgagaagaa cgacaccugg cggcugaagc gcgcccaccu gaucgagaug      660

aagaccugcg aguggccgaa gucccacacg cucuggaccg acgggaucga ggagagcgac      720

cugaucaucc ccaagucccu ggccggcccc cucagccacc acaacacccg ggagggguac      780

cgcacccaga ugaagggccc cuggcacucc gaggagcugg agauccgguu cgaggagugc      840

ccgggcacga agguccacgu ggaggagacc ugcgggaccc gcggccccag ccugcggucc      900

accacggcca gcgggcgcgu caucgaggag uggugcugcc gggagugcac caugcccccg      960

cucuccuucc gcgccaagga cggcugcugg uacggcaugg agauccggcc gcgcaaggag     1020

cccgagagca accuggugcg guccauggug accgcg                               1056


<210>  116
<211>  678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS2A CDS mRNA GC

<400>  116
gggagcaccg accacaugga ccacuucagc cugggcgucc ucgugauccu gcugauggug       60

caggaggggc ucaagaagcg caugacgacc aagaucauca ucuccaccag cauggccguc      120

cugguggcca ugauccuggg cggguucucc augagcgacc ucgccaagcu ggccauccug      180

augggcgcga ccuucgccga gaugaacacc ggcggggacg uggcccaccu cgcccugauc      240

gccgcguuca agguccgccc cgcccugcuc guguccuuca ucuuccgggc caacuggacg      300

ccccgcgaga gcaugcugcu ggcccucgcg uccugccugc ugcagaccgc caucagcgcc      360

cucgagggcg accugauggu gcugaucaac ggguucgccc ucgccuggcu ggcgauccgg      420

gccauggucg ugccgcgcac cgacaacauc acccuggcca uccucgccgc ccugacgccc      480

cuggcgcggg gcacccuccu gguggccugg cgcgccgggc uggccacgug cggcggcuuc      540

augcuccugu cccugaaggg gaagggcagc gucaagaaga accuccccuu cgugauggcc      600

cuggggcuga ccgcggugcg gcucgucgac cccaucaacg uggugggccu gcugcuccug      660

acgcgcuccg ggaagcgg                                                    678


<210>  117
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS2B CDS mRNA GC

<400>  117
agcuggccgc ccuccgaggu ccugaccgcc gugggccuca ucugcgcccu ggccggcggg       60

uucgcgaagg ccgacaucga gauggccggc cccauggccg ccguggggcu gcucaucguc      120

agcuacgugg uguccggcaa gagcgucgac auguacaucg agcgcgcggg ggacaucacc      180

ugggagaagg acgccgaggu gaccggcaac uccccgcggc uggacguggc ccuggacgag      240

agcggcgacu ucucccucgu cgaggacgac gggccgccca ugcgcgagau cauccugaag      300

guggugcuga ugacgaucug cggcaugaac cccaucgcca ucccguucgc cgcgggggcc      360

ugguacgucu acgugaagac cggcaagcgg                                       390


<210>  118
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS3 CDS mRNA GC

<400>  118
agcggcgccc ugugggacgu gcccgccccc aaggagguca agaaggggga gaccaccgac       60

ggcguguacc gcgugaugac gcggcgccuc cuggggagca cccaggucgg cgugggggug      120

augcaggagg gcgucuucca caccaugugg cacgugacca agggcuccgc ccugcggagc      180

ggggagggcc gccucgaccc cuacuggggc gacgugaagc aggaccuggu cuccuacugc      240

gggccgugga agcuggacgc ggccugggac gggcacagcg aggugcagcu ccuggccgug      300

cccccgggcg agcgggcccg caacauccag acgcugcccg gcaucuucaa gaccaaggac      360

ggggacaucg gcgcggucgc ccucgacuac ccggccggga ccuccggcag ccccauccug      420

gacaagugcg ggcgcgugau cggccuguac ggcaacgggg uggucaucaa gaacggcucc      480

uacgugagcg ccaucaccca ggggcggcgc gaggaggaga cgcccgucga gugcuucgag      540

cccuccaugc ucaagaagaa gcagcugacc gugcuggacc uccacccggg cgccgggaag      600

acccggcgcg ugcugcccga gaucguccgg gaggcgauca agacccgccu gcggacggug      660

auccucgccc ccacccgcgu ggucgccgcc gagauggagg aggcccugcg gggccugccc      720

gugcgcuaca ugaccaccgc ggugaacguc acgcacagcg gcaccgagau cguggaccuc      780

augugccacg ccaccuucac cucccggcug cugcagccga uccgcgugcc caacuacaac      840

cucuacauca uggacgaggc ccacuucacg gaccccagcu ccaucgccgc ccggggguac      900

aucagcaccc gcgucgagau gggcgaggcg gccgccaucu ucaugaccgc caccccgccg      960

gggacccggg acgccuuccc cgacuccaac agccccauca uggacacgga gguggaggug     1020

cccgagcgcg cgugguccag cggcuucgac ugggucaccg acuacuccgg caagaccgug     1080

ugguucgugc cgagcguccg gaacgggaac gagaucgccg ccugccugac caaggccggc     1140

aagcgcguga uccagcugag ccggaagacg uucgagaccg aguuccagaa gaccaagcac     1200

caggaguggg acuucguggu caccacggac aucuccgaga ugggggcgaa cuucaaggcc     1260

gaccgcguga ucgacagccg gcgcugccuc aagcccguga uccuggacgg cgagcgcguc     1320

auccuggccg ggcccaugcc cgugacccac gccuccgccg cgcagcggcg cggccggauc     1380

ggccgcaacc cgaacaagcc cggggacgag uaccucuacg gcgggggcug cgccgagacc     1440

gacgaggacc acgcccacug gcuggaggcc cggaugcugc ucgacaacau cuaccugcag     1500

gacgggcuga ucgccagccu cuaccgcccc gaggcggaca agguggccgc caucgagggc     1560

gaguucaagc ugcggaccga gcagcgcaag acguucgucg agcugaugaa gcggggcgac     1620

cucccggugu ggcuggccua ccagguggcc uccgcgggga ucaccuacac cgaccgccgg     1680

uggugcuucg acggcaccac gaacaacacc aucauggagg acagcguccc cgccgaggug     1740

uggacccgcc acggggagaa gcgggugcug aagccccgcu ggauggacgc ccgggucugc     1800

uccgaccacg ccgcgcucaa gagcuucaag gaguucgccg ccggcaagcg c              1851


<210>  119
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS4A CDS mRNA GC

<400>  119
ggggccgccu ucggcgugau ggaggcgcug ggcacccugc ccgggcacau gacggagcgg       60

uuccaggagg ccaucgacaa ccucgccgug cugaugcgcg ccgagaccgg cucccggccg      120

uacaaggccg cggccgccca gcugcccgag acccucgaga ccaucaugcu gcuggggcuc      180

cugggcacgg ucagccuggg caucuucuuc gugcucaugc gcaacaaggg gaucggcaag      240

augggguucg gcauggugac ccugggggcc uccgccuggc ugauguggcu cagcgagauc      300

gagcccgcgc gcaucgccug cguccugauc gugguguucc ugcuccuggu cgugcugauc      360

cccgagccgg agaagcagcg guccccgcag gacaaccaga uggccaucau caucauggug      420

gccgucggcc uccugggccu gaucaccgcg                                       450


<210>  120
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS4B CDS mRNA GC

<400>  120
aacgagcucg gguggcugga gcgcaccaag agcgaccugu cccaccucau gggccggcgc       60

gaggaggggg ccacgauggg cuucagcaug gacaucgacc ugcggcccgc cuccgccugg      120

gccaucuacg cggcccugac caccuucauc acccccgccg ugcagcacgc cgugacgacc      180

agcuacaaca acuacucccu cauggccaug gcgacccagg ccgggguccu guucggcaug      240

ggcaagggga ugccguucua cgccugggac uucggcgugc cgcugcucau gaucgggugc      300

uacagccagc ugaccccgcu gacgcucauc guggccauca uccugcuggu cgcccacuac      360

auguaccuca uccccggccu gcaggcggcc gccgcccgcg ccgcgcagaa gcggaccgcc      420

gccgggauca ugaagaaccc gguggucgac ggcaucgugg ugaccgacau cgacaccaug      480

acgaucgacc cccaggucga gaagaagaug ggccaggugc ugcucauggc cguggcgguc      540

agcuccgcca uccugagccg caccgccugg ggguggggcg aggccggggc ccugaucacc      600

gcggccaccu ccacccucug ggagggcagc cccaacaagu acuggaacuc cagcacggcc      660

accucccugu gcaacaucuu ccgggggagc uaccuggccg gcgccucccu caucuacacc      720

gugacccgca acgcgggccu ggugaagcgg cgc                                   753


<210>  121
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS5 CDS mRNA GC

<400>  121
gggggcggga cgggcgagac ccugggcgag aaguggaagg cccggcucaa ccagaugagc       60

gcccuggagu ucuacuccua caagaagagc gggaucaccg aggucugccg cgaggaggcc      120

cggcgcgccc ugaaggacgg cguggcgacc gggggccacg ccgugucccg ggggagcgcc      180

aagcuccgcu ggcuggucga gcggggcuac cugcagcccu acggcaaggu gaucgaccug      240

gggugcggcc gcgggggcug guccuacuac gccgcgacga uccggaaggu gcaggagguc      300

aagggguaca ccaagggcgg cccggggcac gaggagcccg ugcucgugca gagcuacggc      360

uggaacaucg uccgccugaa guccggggug gacguguucc acauggccgc cgagcccugc      420

gacacccugc ucugcgacau cggcgagagc uccagcagcc ccgaggucga ggaggcccgc      480

acccugcggg ugcuguccau ggugggggac uggcucgaga agcgcccggg cgccuucugc      540

aucaaggucc ugugccccua cacgagcacc augauggaga cccuggagcg gcuccagcgc      600

cgguacggcg ggggccuggu gcgcgugccc cugucccgga acagcaccca cgagauguac      660

ugggucuccg gggcgaagag caacacgauc aaguccguga gcaccaccuc ccagcuccug      720

cugggccgca uggacggccc ccggcgcccg gugaaguacg aggaggacgu caaccucggg      780

agcggcaccc gggccguggu guccugcgcc gaggccccca acaugaagau caucgggaac      840

cgcaucgagc ggauccgcag cgagcacgcc gagacguggu ucuucgacga gaaccacccc      900

uaccggaccu gggcguacca cggcuccuac gaggccccga cccaggggag cgccuccagc      960

cugaucaacg gcgucgugcg ccugcucucc aagcccuggg acguggucac cggcgugacg     1020

gggaucgcca ugaccgacac cacccccuac ggccagcagc ggguguucaa ggagaagguc     1080

gacacgcgcg ugcccgaccc gcaggagggg acccgccagg ugaugagcau ggucagcucc     1140

uggcugugga aggagcuggg caagcacaag cgcccccgcg ugugcaccaa ggaggaguuc     1200

aucaacaagg ugcggagcaa cgccgcgcuc ggggccaucu ucgaggagga gaaggagugg     1260

aagaccgccg ucgaggccgu gaacgacccc cgcuucuggg cccuggugga caaggagcgg     1320

gagcaccacc uccgcggcga gugccagucc ugcgucuaca acaugauggg caagcgggag     1380

aagaagcagg gggaguucgg caaggcgaag gggagccgcg ccaucuggua cauguggcuc     1440

ggcgcccggu uccuggaguu cgaggcccug ggguuccuca acgaggacca cuggaugggc     1500

cgcgagaacu ccggcggggg cguggagggg cugggccugc agcggcucgg guacgugcug     1560

gaggagauga gccgcauccc cggcggccgg auguacgcgg acgacacggc cgggugggac     1620

acccgcaucu cccgguucga ccuggagaac gaggcccuca ucaccaacca gauggagaag     1680

ggccaccgcg cccuggcccu ggcgaucauc aaguacaccu accagaacaa ggucgugaag     1740

guccuccggc cggccgagaa ggggaagacg gugauggaca ucaucagccg ccaggaccag     1800

cggggcuccg gccagguggu caccuacgcc cugaacaccu ucaccaaccu gguggugcag     1860

cucauccgca acauggaggc cgaggagguc cuggagaugc aggaccugug gcuccugcgg     1920

cgcagcgaga aggugaccaa cuggcugcag uccaacgggu gggaccggcu caagcgcaug     1980

gccgugagcg gcgacgacug cgucgugaag cccaucgacg accgcuucgc gcacgcccug     2040

cgguuccuga acgacauggg gaaggugcgc aaggacacgc aggaguggaa gcccuccacc     2100

ggcugggaca acugggagga gguccccuuc ugcagccacc acuucaacaa gcuccaccug     2160

aaggacgggc gguccaucgu ggugccgugc cgccaccagg acgagcugau cggccgggcc     2220

cgcgucagcc ccggcgccgg gugguccauc cgggagaccg ccugccucgc gaagagcuac     2280

gcccagaugu ggcagcugcu guacuuccac cgccgggacc uccgccugau ggccaacgcc     2340

aucugcucca gcgugcccgu ggacuggguc cccaccggcc ggacgaccug gagcauccac     2400

gggaagggcg aguggaugac caccgaggac augcuggugg uguggaaccg cgucuggauc     2460

gaggagaacg accacaugga ggacaagacg ccggugacca aguggaccga cauccccuac     2520

cucgggaagc gggaggaccu guggugcggc ucccugaucg gccaccgccc ccggaccacg     2580

ugggcggaga acaucaagaa caccgugaac augguccgcc ggaucaucgg ggacgaggag     2640

aaguacaugg acuaccucag cacccaggug cgcuaccugg gcgaggaggg guccaccccc     2700

ggcgugcug                                                             2709


<210>  122
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS mRNA GC

<400>  122
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cuccgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaag                               2136


<210>  123
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS mRNA GC

<400>  123
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

aucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac ggucagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga agguggagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc accccgcacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgugg ucgugcucgg gucccaggag     1380

ggcgccgugc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucagcuacu cccugugcac ggcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggugga gguccaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cgguggacau gcagacccug acccccgucg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgccguuc     1740

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcgcccugaa cucccucggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  124
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS mRNA GC

<400>  124
agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc       60

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      120

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      180

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      300

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      360

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      420

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      480

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      600

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      660

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg      720

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag      840

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc      900

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg      960

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1020

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1080

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1140

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1320

gugcuggggg acaccgcgug ggacuucggc agc                                  1353


<210>  125
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS mRNA GC

<400>  125
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

aucgugcugg agcacggcgg gugcgugacc gucauggcgc aggacaagcc gacgguggac      180

aucgagcucg ugaccaccac cgucagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      360

aacggcugcg ggcucuucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

gugcacggcu cccagcacag cgggaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcugggcggg      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgcgaagcgc cagaccgugg ugguccuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gugagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ucacggugga gguccaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug accccggucg ggcgccucau cacggccaac     1140

ccggugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  126
<211>  10269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein CDS mRNA GC

<400>  126
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc      360

auggcggccg aggucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      420

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      480

auggaccugg ggcacacgug cgacgcgacc auguccuacg agugccccau gcucgacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca cgagcaccug ggucguguac      600

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      660

agccacucca cgcgcaagcu gcagacccgg agccagaccu ggcucgaguc ccgcgaguac      720

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      780

gcggccgcca ucgccuggcu ccuggggagc uccacgagcc agaaggugau cuaccuggug      840

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      900

uucguggagg ggauguccgg cggcaccugg guggacgucg ugcuggagca cgggggcugc      960

gugaccguca uggcccagga caagcccacc guggacaucg agcucgugac gaccaccguc     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggccu ccaucagcga cauggccucc     1080

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacgcaguac     1140

gucugcaagc gcacccuggu ggaccggggc ugggggaacg gcugcggccu cuucgggaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc     1260

cagccggaga accuggagua ccgcaucaug cucuccgucc acggcagcca gcacuccggg     1320

augaucguga acgacacggg ccacgagacc gacgagaacc gggccaaggu ggagaucacc     1380

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1440

gagccccgga cgggccugga cuucagcgac cuguacuacc ucaccaugaa caacaagcac     1500

uggcuggucc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1560

accgggaccc cgcacuggaa caacaaggag gcccucgugg aguucaagga cgcgcacgcc     1620

aagcgccaga cgguggucgu gcugggcucc caggaggggg ccgugcacac cgcccuggcc     1680

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1740

ugccgccuga agauggacaa gcuccggcug aaggggguca gcuacucccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgcggag acgcuccacg gcaccgugac cguggagguc     1860

caguacgccg ggaccgacgg cccgugcaag gugcccgccc agauggccgu ggacaugcag     1920

acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg ugaucaccga gagcacggag     1980

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     2040

gucggggaga agaagaucac ccaccacugg caccggagcg gcuccaccau cgggaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc ugggggacac ggcgugggac     2160

uucggcagcg ucggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     2220

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacc     2280

cugcugaugu ggcucgggcu gaacgccaag aacggcucca ucagccugau gugccucgcg     2340

cugggcggcg ugcugaucuu ccucuccacc gccgugagcg ccgacgucgg gugcuccgug     2400

gacuucagca agaaggagac ccgcugcggg acgggcgugu ucgucuacaa cgacguggag     2460

gccuggcggg accgcuacaa guaccacccc gacuccccgc gccggcuggc cgcggccgug     2520

aagcaggccu gggaggacgg gaucugcggc aucagcuccg ucagccgcau ggagaacauc     2580

auguggcgga gcguggaggg ggagcugaac gccauccucg aggagaacgg cgugcagcug     2640

accgucgugg ugggcuccgu caagaacccg auguggcgcg ggccccagcg gcugcccgug     2700

ccggugaacg agcuccccca cggcuggaag gcguggggga agagcuacuu cguccgcgcc     2760

gccaagacca acaacuccuu cgugguggac ggcgacaccc ugaaggagug cccccugaag     2820

caccgggccu ggaacagcuu ccucgucgag gaccacgggu ucggcguguu ccacacgucc     2880

guguggcuga agguccgcga ggacuacagc cuggagugcg accccgccgu gaucggcacc     2940

gcggugaagg ggaaggaggc cguccacucc gaccucggcu acuggaucga gagcgagaag     3000

aacgacaccu ggcggcugaa gcgcgcccac cugaucgaga ugaagaccug cgaguggccg     3060

aagucccaca cgcucuggac cgacgggauc gaggagagcg accugaucau ccccaagucc     3120

cuggccggcc cccucagcca ccacaacacc cgggaggggu accgcaccca gaugaagggc     3180

cccuggcacu ccgaggagcu ggagauccgg uucgaggagu gcccgggcac gaaggugcac     3240

gucgaggaga ccugcgggac ccgcggcccc agccugcggu ccaccacggc cagcgggcgc     3300

gugaucgagg aguggugcug ccgggagugc accaugccgc cccucuccuu ccgcgcgaag     3360

gacggcugcu gguacggcau ggagauccgg ccgcgcaagg agcccgagag caaccuggug     3420

cgguccaugg ucaccgccgg gagcaccgac cacauggacc acuucagccu gggcgugcuc     3480

gugauccugc ugauggucca ggaggggcuc aagaagcgca ugacgaccaa gaucaucauc     3540

uccaccagca uggccgugcu gguggccaug auccugggcg gguucuccau gagcgaccuc     3600

gccaagcugg cgauccugau gggcgccacc uucgccgaga ugaacaccgg cggggacguc     3660

gcccaccucg cccugaucgc ggccuucaag gugcgccccg cccugcucgu guccuucauc     3720

uuccgggcca acuggacgcc ccgcgagagc augcugcugg cgcucgccuc cugccugcug     3780

cagaccgcca ucagcgcccu cgagggcgac cugauggucc ugaucaacgg guucgcccuc     3840

gcguggcugg ccauccgggc caugguggug ccgcgcaccg acaacaucac ccuggccauc     3900

cucgccgcgc ugacgccccu ggcccggggc acccuccugg ucgccuggcg cgccgggcug     3960

gccacgugcg gcggcuucau gcuccugucc cugaagggga agggcagcgu gaagaagaac     4020

cuccccuucg ugauggcgcu ggggcugacc gccguccggc ucguggaccc caucaacgug     4080

gucggccugc ugcuccugac gcgcuccggg aagcggagcu ggccgcccuc cgaggugcug     4140

accgccgugg gccucaucug cgcccuggcg ggcggguucg ccaaggccga caucgagaug     4200

gccggcccca uggccgcggu cgggcugcuc aucgugagcu acguggucuc cggcaagagc     4260

guggacaugu acaucgagcg cgccggggac aucaccuggg agaaggacgc cgaggugacc     4320

ggcaacuccc cgcggcugga cgucgcccug gacgagagcg gcgacuucuc ccucguggag     4380

gacgacgggc cgcccaugcg cgagaucauc cugaaggugg uccugaugac gaucugcggc     4440

augaacccca ucgccauccc guucgcggcc ggggccuggu acguguacgu gaagaccggc     4500

aagcggagcg gcgcccugug ggacgucccc gcccccaagg aggugaagaa gggggagacc     4560

accgacggcg uguaccgcgu caugacgcgg cgccuccugg ggagcaccca ggugggcgug     4620

ggggucaugc aggagggcgu guuccacacc auguggcacg ugaccaaggg cuccgcgcug     4680

cggagcgggg agggccgccu cgaccccuac uggggcgacg ucaagcagga ccuggugucc     4740

uacugcgggc cguggaagcu ggacgccgcc ugggacgggc acagcgaggu gcagcuccug     4800

gccgucccgc ccggcgagcg ggcgcgcaac auccagacgc ugcccggcau cuucaagacc     4860

aaggacgggg acaucggcgc cguggcccuc gacuacccgg ccgggaccuc cggcagcccc     4920

auccuggaca agugcgggcg cgugaucggc cuguacggca acggggucgu gaucaagaac     4980

ggcuccuacg ucagcgccau cacccagggg cggcgcgagg aggagacgcc cguggagugc     5040

uucgagcccu ccaugcucaa gaagaagcag cugaccgugc uggaccucca cccgggcgcg     5100

gggaagaccc ggcgcguccu gcccgagauc gugcgggagg ccaucaagac ccgccugcgg     5160

acggugaucc ucgcccccac ccgcgucgug gccgccgaga uggaggaggc gcugcggggc     5220

cugcccgugc gcuacaugac caccgccguc aacgugacgc acagcggcac cgagaucgug     5280

gaccucaugu gccacgccac cuucaccucc cggcugcugc agccgauccg cguccccaac     5340

uacaaccucu acaucaugga cgaggcccac uucacggacc ccagcuccau cgccgcgcgg     5400

ggguacauca gcacccgcgu ggagaugggc gaggccgccg ccaucuucau gaccgccacc     5460

ccgccgggga cccgggacgc guuccccgac uccaacagcc ccaucaugga cacggaggug     5520

gagguccccg agcgcgccug guccagcggc uucgacuggg ugaccgacca cuccggcaag     5580

accguguggu ucgucccgag cgugcggaac gggaacgaga ucgccgccug ccugaccaag     5640

gcgggcaagc gcgugaucca gcugagccgg aagacguucg agaccgaguu ccagaagacc     5700

aagcaccagg agugggacuu cgucgugacc acggacaucu ccgagauggg ggccaacuuc     5760

aaggccgacc gcgugaucga cagccggcgc ugccucaagc ccgucauccu ggacggcgag     5820

cgcgugaucc uggccgggcc caugcccgug acccacgccu ccgcggccca gcggcgcggc     5880

cggaucggcc gcaacccgaa caagcccggg gacgaguacc ucuacggcgg gggcugcgcc     5940

gagaccgacg aggaccacgc ccacuggcug gaggcccgga ugcugcucga caacaucuac     6000

cugcaggacg ggcugaucgc gagccucuac cgccccgagg ccgacaaggu cgccgccauc     6060

gagggcgagu ucaagcugcg gaccgagcag cgcaagacgu ucguggagcu gaugaagcgg     6120

ggcgaccucc cgguguggcu ggccuaccag gucgcguccg ccgggaucac cuacaccgac     6180

cgccgguggu gcuucgacgg caccacgaac aacaccauca uggaggacag cgugcccgcc     6240

gaggugugga cccgccacgg ggagaagcgg guccugaagc cccgcuggau ggacgcccgg     6300

gugugcuccg accacgcggc ccucaagagc uucaaggagu ucgccgccgg caagcgcggg     6360

gccgcguucg gcgugaugga ggcccugggc acccugcccg ggcacaugac ggagcgguuc     6420

caggaggcca ucgacaaccu cgccguccug augcgcgccg agaccggcuc ccggccguac     6480

aaggcggccg ccgcccagcu gcccgagacc cucgagacca ucaugcugcu ggggcuccug     6540

ggcacgguga gccugggcau cuucuucgug cucaugcgca acaaggggau cggcaagaug     6600

ggguucggca uggucacccu gggggccucc gcguggcuga uguggcucag cgagaucgag     6660

cccgcccgca ucgccugcgu gcugaucgug gucuuccugc uccugguggu gcugaucccc     6720

gagccggaga agcagcgguc cccgcaggac aaccagaugg ccaucaucau cauggucgcg     6780

gugggccucc ugggccugau caccgccaac gagcucgggu ggcuggagcg caccaagagc     6840

gaccuguccc accucauggg ccggcgcgag gagggggcca cgaucggcuu cagcauggac     6900

aucgaccugc ggcccgccuc cgccugggcg aucuacgccg cccugaccac cuucaucacc     6960

cccgccgugc agcacgccgu cacgaccagc uacaacaacu acucccucau ggcgauggcc     7020

acccaggccg gggugcuguu cggcaugggc aaggggaugc cguucuacgc cugggacuuc     7080

ggcgugcccc ugcucaugau cgggugcuac agccagcuga ccccgcugac gcucaucguc     7140

gccaucaucc ugcugguggc gcacuacaug uaccucaucc ccggccugca ggccgccgcc     7200

gcccgcgcgg cccagaagcg gaccgccgcc gggaucauga agaacccggu cguggacggc     7260

aucgugguca ccgacaucga caccaugacg aucgaccccc agguggagaa gaagaugggc     7320

caggugcugc ucaucgcggu cgccgugagc uccgccaucc ugagccgcac cgccuggggg     7380

uggggcgagg ccggggcgcu gaucaccgcc gccaccucca cccucuggga gggcagcccc     7440

aacaaguacu ggaacuccag cacggccacc ucccugugca acaucuuccg ggggagcuac     7500

cuggccggcg cgucccucau cuacaccgug acccgcaacg ccggccuggu caagcggcgc     7560

gggggcggga cgggcgagac ccugggcgag aaguggaagg cccggcucaa ccagaugagc     7620

gcccuggagu ucuacuccua caagaagagc gggaucaccg aggugugccg cgaggaggcc     7680

cggcgcgcgc ugaaggacgg cguggccacc gggggccacg ccgucucccg ggggagcgcc     7740

aagcuccgcu ggcuggugga gcggggcuac cugcagcccu acggcaaggu gaucgaccug     7800

gggugcggcc gcgggggcug guccuacuac gcggccacga uccggaaggu ccaggaggug     7860

aagggguaca ccaagggcgg cccggggcac gaggagcccg ugcucgucca gagcuacggc     7920

uggaacaucg ugcgccugaa guccggggug gacgucuucc acauggccgc cgagcccugc     7980

gacacccugc ucugcgacau cggcgagagc uccagcagcc ccgaggugga ggaggcccgc     8040

acccugcggg ugcuguccau ggucggggac uggcucgaga agcgcccggg cgcguucugc     8100

aucaaggugc ugugccccua cacgagcacc augauggaga cccuggagcg gcuccagcgc     8160

cgguacggcg ggggccuggu gcgcgucccc cugucccgga acagcaccca cgagauguac     8220

uggguguccg gggccaagag caacacgauc aaguccguga gcaccaccuc ccagcuccug     8280

cugggccgca uggacggccc ccggcgcccg gucaaguacg aggaggacgu gaaccucggg     8340

agcggcaccc gggccguggu cuccugcgcc gaggccccca acaugaagau caucgggaac     8400

cgcaucgagc ggauccgcag cgagcacgcg gagacguggu ucuucgacga gaaccacccc     8460

uaccggaccu gggccuacca cggcuccuac gaggccccga cccaggggag cgccuccagc     8520

cugaucaacg gcguggugcg ccugcucucc aagcccuggg acgucgugac cggcgugacg     8580

gggaucgcca ugaccgacac cacccccuac ggccagcagc gggucuucaa ggagaaggug     8640

gacacgcgcg ugcccgaccc gcaggagggg acccggcagg ucaugagcau ggugagcucc     8700

uggcugugga aggagcuggg caagcacaag cgcccccgcg ugugcaccaa ggaggaguuc     8760

aucaacaagg uccggagcaa cgcggcccuc ggggccaucu ucgaggagga gaaggagugg     8820

aagaccgccg uggaggccgu gaacgacccc cgcuucuggg cgcuggucga caaggagcgg     8880

gagcaccacc uccgcggcga gugccagucc ugcguguaca acaugauggg caagcgggag     8940

aagaagcagg gggaguucgg caaggccaag gggagccgcg ccaucuggua cauguggcuc     9000

ggcgcccggu uccuggaguu cgaggcgcug ggguuccuca acgaggacca cuggaugggc     9060

cgcgagaacu ccggcggggg cguggagggg cugggccugc agcggcucgg guacguccug     9120

gaggagauga gccgcauccc cggcggccgg auguacgccg acgacacggc cgggugggac     9180

acccgcaucu cccgguucga ccuggagaac gaggcccuca ucaccaacca gauggagaag     9240

ggccaccgcg cccuggcgcu ggccaucauc aaguacaccu accagaacaa gguggucaag     9300

gugcuccggc cggccgagaa ggggaagacg gugauggaca ucaucagccg ccaggaccag     9360

cggggcuccg gccaggucgu gaccuacgcc cugaacaccu ucaccaaccu ggugguccag     9420

cucauccgca acauggaggc cgaggaggug cuggagaugc aggaccugug gcuccugcgg     9480

cgcagcgaga aggugaccaa cuggcugcag uccaacgggu gggaccggcu caagcgcaug     9540

gcggucagcg gcgacgacug cguggugaag cccaucgacg accgcuucgc ccacgcccug     9600

cgguuccuga acgacauggg gaagguccgc aaggacacgc aggaguggaa gcccuccacc     9660

ggcugggaca acugggagga ggugcccuuc ugcagccacc acuucaacaa gcuccaccug     9720

aaggacgggc gguccaucgu ggucccgugc cgccaccagg acgagcugau cggccgggcc     9780

cgcgugagcc ccggcgccgg gugguccauc cgggagaccg cgugccucgc caagagcuac     9840

gcccagaugu ggcagcugcu guacuuccac cgccgggacc uccgccugau ggccaacgcg     9900

aucugcucca gcgugcccgu cgacugggug cccaccggcc ggacgaccug gagcauccac     9960

gggaagggcg aguggaugac caccgaggac augcuggugg ucuggaaccg cguguggauc    10020

gaggagaacg accacaugga ggacaagacg ccggugacca aguggaccga cauccccuac    10080

cucgggaagc gggaggaccu guggugcggc ucccugaucg gccaccgccc ccggaccacg    10140

ugggccgaga acaucaagaa caccgucaac auggugcgcc ggaucaucgg ggacgaggag    10200

aaguacaugg acuaccucag cacccaggug cgcuaccugg gcgaggaggg guccaccccc    10260

ggcguccug                                                            10269


<210>  127
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C CDS mRNA GC

<400>  127
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gc                                                          312


<210>  128
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-ER anchor CDS mRNA GC

<400>  128
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc      360

auggcg                                                                 366


<210>  129
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) pr CDS mRNA GC

<400>  129
gccgagguca cccggcgcgg cuccgccuac uacauguacc ucgaccggaa cgacgccggg       60

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      120

cuggggcaca cgugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accacgagca ccugggucgu guacggcacg      240

ugccaccaca agaaggggga ggcccgccgg ucccgccgg                             279


<210>  130
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) M CDS mRNA GC

<400>  130
gccgugaccc ugcccagcca cuccacgcgc aagcugcaga cccggagcca gaccuggcuc       60

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      120

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac gagccagaag      180

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acucc                      225


<210>  131
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prM CDS mRNA GC

<400>  131
gccgagguca cccggcgcgg cuccgccuac uacauguacc ucgaccggaa cgacgccggg       60

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      120

cuggggcaca cgugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accacgagca ccugggucgu guacggcacg      240

ugccaccaca agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac      300

uccacgcgca agcugcagac ccggagccag accuggcucg agucccgcga guacaccaag      360

caccugaucc gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc      420

gccaucgccu ggcuccuggg gagcuccacg agccagaagg ugaucuaccu ggugaugauc      480

cuccugaucg cccccgcgua cucc                                             504


<210>  132
<211>  1512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) E CDS mRNA GC

<400>  132
auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg       60

guggacgucg ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc      120

guggacaucg agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc      180

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      240

uaccuggaca agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc      300

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      360

ugcuccaaga agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug      420

cucuccgucc acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc      480

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug      540

ggcggguucg gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac      600

cuguacuacc ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac      660

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag      720

gcccucgugg aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc      780

caggaggggg ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc      840

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug      900

aaggggguca gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag      960

acgcuccacg gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag     1020

gugcccgccc agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc     1080

gcgaaccccg ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg     1140

cccuucggcg acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg     1200

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg     1260

auggccgugc ugggggacac ggcgugggac uucggcagcg ucggcggggc ccucaacucc     1320

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1380

uccugguuca gccagauccu caucggcacc cugcugaugu ggcucgggcu gaacgccaag     1440

aacggcucca ucagccugau gugccucgcg cugggcggcg ugcugaucuu ccucuccacc     1500

gccgugagcg cc                                                         1512


<210>  133
<211>  2016
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME CDS mRNA GC

<400>  133
gccgagguca cccggcgcgg cuccgccuac uacauguacc ucgaccggaa cgacgccggg       60

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      120

cuggggcaca cgugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accacgagca ccugggucgu guacggcacg      240

ugccaccaca agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac      300

uccacgcgca agcugcagac ccggagccag accuggcucg agucccgcga guacaccaag      360

caccugaucc gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc      420

gccaucgccu ggcuccuggg gagcuccacg agccagaagg ugaucuaccu ggugaugauc      480

cuccugaucg cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug      540

gaggggaugu ccggcggcac cuggguggac gucgugcugg agcacggggg cugcgugacc      600

gucauggccc aggacaagcc caccguggac aucgagcucg ugacgaccac cgucagcaac      660

auggccgagg ugcgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc      720

cggugcccca cccaggggga ggcguaccug gacaagcagu ccgacacgca guacgucugc      780

aagcgcaccc ugguggaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc      840

cuggugaccu gcgccaaguu cgccugcucc aagaagauga ccgggaagag cauccagccg      900

gagaaccugg aguaccgcau caugcucucc guccacggca gccagcacuc cgggaugauc      960

gugaacgaca cgggccacga gaccgacgag aaccgggcca agguggagau cacccccaac     1020

agcccccgcg cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc     1080

cggacgggcc uggacuucag cgaccuguac uaccucacca ugaacaacaa gcacuggcug     1140

guccacaagg agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg     1200

accccgcacu ggaacaacaa ggaggcccuc guggaguuca aggacgcgca cgccaagcgc     1260

cagacggugg ucgugcuggg cucccaggag ggggccgugc acaccgcccu ggccggcgcg     1320

cuggaggccg agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc     1380

cugaagaugg acaagcuccg gcugaagggg gucagcuacu cccugugcac cgccgccuuc     1440

accuucacca agauccccgc ggagacgcuc cacggcaccg ugaccgugga gguccaguac     1500

gccgggaccg acggcccgug caaggugccc gcccagaugg ccguggacau gcagacgcug     1560

acccccgucg ggcgccugau caccgcgaac cccgugauca ccgagagcac ggagaacagc     1620

aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgucggg     1680

gagaagaaga ucacccacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag     1740

gccaccgugc gcggcgccaa gcggauggcc gugcuggggg acacggcgug ggacuucggc     1800

agcgucggcg gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc     1860

uucaagagcc uguucggggg cauguccugg uucagccaga uccucaucgg cacccugcug     1920

auguggcucg ggcugaacgc caagaacggc uccaucagcc ugaugugccu cgcgcugggc     1980

ggcgugcuga ucuuccucuc caccgccgug agcgcc                               2016


<210>  134
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS1 CDS mRNA GC

<400>  134
gacgucgggu gcuccgugga cuucagcaag aaggagaccc gcugcgggac gggcguguuc       60

gucuacaacg acguggaggc cuggcgggac cgcuacaagu accaccccga cuccccgcgc      120

cggcuggccg cggccgugaa gcaggccugg gaggacggga ucugcggcau cagcuccguc      180

agccgcaugg agaacaucau guggcggagc guggaggggg agcugaacgc cauccucgag      240

gagaacggcg ugcagcugac cgucguggug ggcuccguca agaacccgau guggcgcggg      300

ccccagcggc ugcccgugcc ggugaacgag cucccccacg gcuggaaggc gugggggaag      360

agcuacuucg uccgcgccgc caagaccaac aacuccuucg ugguggacgg cgacacccug      420

aaggagugcc cccugaagca ccgggccugg aacagcuucc ucgucgagga ccacggguuc      480

ggcguguucc acacguccgu guggcugaag guccgcgagg acuacagccu ggagugcgac      540

cccgccguga ucggcaccgc ggugaagggg aaggaggccg uccacuccga ccucggcuac      600

uggaucgaga gcgagaagaa cgacaccugg cggcugaagc gcgcccaccu gaucgagaug      660

aagaccugcg aguggccgaa gucccacacg cucuggaccg acgggaucga ggagagcgac      720

cugaucaucc ccaagucccu ggccggcccc cucagccacc acaacacccg ggagggguac      780

cgcacccaga ugaagggccc cuggcacucc gaggagcugg agauccgguu cgaggagugc      840

ccgggcacga aggugcacgu cgaggagacc ugcgggaccc gcggccccag ccugcggucc      900

accacggcca gcgggcgcgu gaucgaggag uggugcugcc gggagugcac caugccgccc      960

cucuccuucc gcgcgaagga cggcugcugg uacggcaugg agauccggcc gcgcaaggag     1020

cccgagagca accuggugcg guccaugguc accgcc                               1056


<210>  135
<211>  678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS2A CDS mRNA GC

<400>  135
gggagcaccg accacaugga ccacuucagc cugggcgugc ucgugauccu gcugaugguc       60

caggaggggc ucaagaagcg caugacgacc aagaucauca ucuccaccag cauggccgug      120

cugguggcca ugauccuggg cggguucucc augagcgacc ucgccaagcu ggcgauccug      180

augggcgcca ccuucgccga gaugaacacc ggcggggacg ucgcccaccu cgcccugauc      240

gcggccuuca aggugcgccc cgcccugcuc guguccuuca ucuuccgggc caacuggacg      300

ccccgcgaga gcaugcugcu ggcgcucgcc uccugccugc ugcagaccgc caucagcgcc      360

cucgagggcg accugauggu ccugaucaac ggguucgccc ucgcguggcu ggccauccgg      420

gccauggugg ugccgcgcac cgacaacauc acccuggcca uccucgccgc gcugacgccc      480

cuggcccggg gcacccuccu ggucgccugg cgcgccgggc uggccacgug cggcggcuuc      540

augcuccugu cccugaaggg gaagggcagc gugaagaaga accuccccuu cgugauggcg      600

cuggggcuga ccgccguccg gcucguggac cccaucaacg uggucggccu gcugcuccug      660

acgcgcuccg ggaagcgg                                                    678


<210>  136
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS2B CDS mRNA GC

<400>  136
agcuggccgc ccuccgaggu gcugaccgcc gugggccuca ucugcgcccu ggcgggcggg       60

uucgccaagg ccgacaucga gauggccggc cccauggccg cggucgggcu gcucaucgug      120

agcuacgugg ucuccggcaa gagcguggac auguacaucg agcgcgccgg ggacaucacc      180

ugggagaagg acgccgaggu gaccggcaac uccccgcggc uggacgucgc ccuggacgag      240

agcggcgacu ucucccucgu ggaggacgac gggccgccca ugcgcgagau cauccugaag      300

gugguccuga ugacgaucug cggcaugaac cccaucgcca ucccguucgc ggccggggcc      360

ugguacgugu acgugaagac cggcaagcgg                                       390


<210>  137
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS3 CDS mRNA GC

<400>  137
agcggcgccc ugugggacgu ccccgccccc aaggagguga agaaggggga gaccaccgac       60

ggcguguacc gcgucaugac gcggcgccuc cuggggagca cccagguggg cguggggguc      120

augcaggagg gcguguucca caccaugugg cacgugacca agggcuccgc gcugcggagc      180

ggggagggcc gccucgaccc cuacuggggc gacgucaagc aggaccuggu guccuacugc      240

gggccgugga agcuggacgc cgccugggac gggcacagcg aggugcagcu ccuggccguc      300

ccgcccggcg agcgggcgcg caacauccag acgcugcccg gcaucuucaa gaccaaggac      360

ggggacaucg gcgccguggc ccucgacuac ccggccggga ccuccggcag ccccauccug      420

gacaagugcg ggcgcgugau cggccuguac ggcaacgggg ucgugaucaa gaacggcucc      480

uacgucagcg ccaucaccca ggggcggcgc gaggaggaga cgcccgugga gugcuucgag      540

cccuccaugc ucaagaagaa gcagcugacc gugcuggacc uccacccggg cgcggggaag      600

acccggcgcg uccugcccga gaucgugcgg gaggccauca agacccgccu gcggacggug      660

auccucgccc ccacccgcgu cguggccgcc gagauggagg aggcgcugcg gggccugccc      720

gugcgcuaca ugaccaccgc cgucaacgug acgcacagcg gcaccgagau cguggaccuc      780

augugccacg ccaccuucac cucccggcug cugcagccga uccgcguccc caacuacaac      840

cucuacauca uggacgaggc ccacuucacg gaccccagcu ccaucgccgc gcggggguac      900

aucagcaccc gcguggagau gggcgaggcc gccgccaucu ucaugaccgc caccccgccg      960

gggacccggg acgcguuccc cgacuccaac agccccauca uggacacgga gguggagguc     1020

cccgagcgcg ccugguccag cggcuucgac ugggugaccg accacuccgg caagaccgug     1080

ugguucgucc cgagcgugcg gaacgggaac gagaucgccg ccugccugac caaggcgggc     1140

aagcgcguga uccagcugag ccggaagacg uucgagaccg aguuccagaa gaccaagcac     1200

caggaguggg acuucgucgu gaccacggac aucuccgaga ugggggccaa cuucaaggcc     1260

gaccgcguga ucgacagccg gcgcugccuc aagcccguca uccuggacgg cgagcgcgug     1320

auccuggccg ggcccaugcc cgugacccac gccuccgcgg cccagcggcg cggccggauc     1380

ggccgcaacc cgaacaagcc cggggacgag uaccucuacg gcgggggcug cgccgagacc     1440

gacgaggacc acgcccacug gcuggaggcc cggaugcugc ucgacaacau cuaccugcag     1500

gacgggcuga ucgcgagccu cuaccgcccc gaggccgaca aggucgccgc caucgagggc     1560

gaguucaagc ugcggaccga gcagcgcaag acguucgugg agcugaugaa gcggggcgac     1620

cucccggugu ggcuggccua ccaggucgcg uccgccggga ucaccuacac cgaccgccgg     1680

uggugcuucg acggcaccac gaacaacacc aucauggagg acagcgugcc cgccgaggug     1740

uggacccgcc acggggagaa gcggguccug aagccccgcu ggauggacgc ccgggugugc     1800

uccgaccacg cggcccucaa gagcuucaag gaguucgccg ccggcaagcg c              1851


<210>  138
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS4A CDS mRNA GC

<400>  138
ggggccgcgu ucggcgugau ggaggcccug ggcacccugc ccgggcacau gacggagcgg       60

uuccaggagg ccaucgacaa ccucgccguc cugaugcgcg ccgagaccgg cucccggccg      120

uacaaggcgg ccgccgccca gcugcccgag acccucgaga ccaucaugcu gcuggggcuc      180

cugggcacgg ugagccuggg caucuucuuc gugcucaugc gcaacaaggg gaucggcaag      240

augggguucg gcauggucac ccugggggcc uccgcguggc ugauguggcu cagcgagauc      300

gagcccgccc gcaucgccug cgugcugauc guggucuucc ugcuccuggu ggugcugauc      360

cccgagccgg agaagcagcg guccccgcag gacaaccaga uggccaucau caucaugguc      420

gcggugggcc uccugggccu gaucaccgcc                                       450


<210>  139
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS4B CDS mRNA GC

<400>  139
aacgagcucg gguggcugga gcgcaccaag agcgaccugu cccaccucau gggccggcgc       60

gaggaggggg ccacgaucgg cuucagcaug gacaucgacc ugcggcccgc cuccgccugg      120

gcgaucuacg ccgcccugac caccuucauc acccccgccg ugcagcacgc cgucacgacc      180

agcuacaaca acuacucccu cauggcgaug gccacccagg ccggggugcu guucggcaug      240

ggcaagggga ugccguucua cgccugggac uucggcgugc cccugcucau gaucgggugc      300

uacagccagc ugaccccgcu gacgcucauc gucgccauca uccugcuggu ggcgcacuac      360

auguaccuca uccccggccu gcaggccgcc gccgcccgcg cggcccagaa gcggaccgcc      420

gccgggauca ugaagaaccc ggucguggac ggcaucgugg ucaccgacau cgacaccaug      480

acgaucgacc cccaggugga gaagaagaug ggccaggugc ugcucaucgc ggucgccgug      540

agcuccgcca uccugagccg caccgccugg ggguggggcg aggccggggc gcugaucacc      600

gccgccaccu ccacccucug ggagggcagc cccaacaagu acuggaacuc cagcacggcc      660

accucccugu gcaacaucuu ccgggggagc uaccuggccg gcgcgucccu caucuacacc      720

gugacccgca acgccggccu ggucaagcgg cgc                                   753


<210>  140
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS5 CDS mRNA GC

<400>  140
gggggcggga cgggcgagac ccugggcgag aaguggaagg cccggcucaa ccagaugagc       60

gcccuggagu ucuacuccua caagaagagc gggaucaccg aggugugccg cgaggaggcc      120

cggcgcgcgc ugaaggacgg cguggccacc gggggccacg ccgucucccg ggggagcgcc      180

aagcuccgcu ggcuggugga gcggggcuac cugcagcccu acggcaaggu gaucgaccug      240

gggugcggcc gcgggggcug guccuacuac gcggccacga uccggaaggu ccaggaggug      300

aagggguaca ccaagggcgg cccggggcac gaggagcccg ugcucgucca gagcuacggc      360

uggaacaucg ugcgccugaa guccggggug gacgucuucc acauggccgc cgagcccugc      420

gacacccugc ucugcgacau cggcgagagc uccagcagcc ccgaggugga ggaggcccgc      480

acccugcggg ugcuguccau ggucggggac uggcucgaga agcgcccggg cgcguucugc      540

aucaaggugc ugugccccua cacgagcacc augauggaga cccuggagcg gcuccagcgc      600

cgguacggcg ggggccuggu gcgcgucccc cugucccgga acagcaccca cgagauguac      660

uggguguccg gggccaagag caacacgauc aaguccguga gcaccaccuc ccagcuccug      720

cugggccgca uggacggccc ccggcgcccg gucaaguacg aggaggacgu gaaccucggg      780

agcggcaccc gggccguggu cuccugcgcc gaggccccca acaugaagau caucgggaac      840

cgcaucgagc ggauccgcag cgagcacgcg gagacguggu ucuucgacga gaaccacccc      900

uaccggaccu gggccuacca cggcuccuac gaggccccga cccaggggag cgccuccagc      960

cugaucaacg gcguggugcg ccugcucucc aagcccuggg acgucgugac cggcgugacg     1020

gggaucgcca ugaccgacac cacccccuac ggccagcagc gggucuucaa ggagaaggug     1080

gacacgcgcg ugcccgaccc gcaggagggg acccggcagg ucaugagcau ggugagcucc     1140

uggcugugga aggagcuggg caagcacaag cgcccccgcg ugugcaccaa ggaggaguuc     1200

aucaacaagg uccggagcaa cgcggcccuc ggggccaucu ucgaggagga gaaggagugg     1260

aagaccgccg uggaggccgu gaacgacccc cgcuucuggg cgcuggucga caaggagcgg     1320

gagcaccacc uccgcggcga gugccagucc ugcguguaca acaugauggg caagcgggag     1380

aagaagcagg gggaguucgg caaggccaag gggagccgcg ccaucuggua cauguggcuc     1440

ggcgcccggu uccuggaguu cgaggcgcug ggguuccuca acgaggacca cuggaugggc     1500

cgcgagaacu ccggcggggg cguggagggg cugggccugc agcggcucgg guacguccug     1560

gaggagauga gccgcauccc cggcggccgg auguacgccg acgacacggc cgggugggac     1620

acccgcaucu cccgguucga ccuggagaac gaggcccuca ucaccaacca gauggagaag     1680

ggccaccgcg cccuggcgcu ggccaucauc aaguacaccu accagaacaa gguggucaag     1740

gugcuccggc cggccgagaa ggggaagacg gugauggaca ucaucagccg ccaggaccag     1800

cggggcuccg gccaggucgu gaccuacgcc cugaacaccu ucaccaaccu ggugguccag     1860

cucauccgca acauggaggc cgaggaggug cuggagaugc aggaccugug gcuccugcgg     1920

cgcagcgaga aggugaccaa cuggcugcag uccaacgggu gggaccggcu caagcgcaug     1980

gcggucagcg gcgacgacug cguggugaag cccaucgacg accgcuucgc ccacgcccug     2040

cgguuccuga acgacauggg gaagguccgc aaggacacgc aggaguggaa gcccuccacc     2100

ggcugggaca acugggagga ggugcccuuc ugcagccacc acuucaacaa gcuccaccug     2160

aaggacgggc gguccaucgu ggucccgugc cgccaccagg acgagcugau cggccgggcc     2220

cgcgugagcc ccggcgccgg gugguccauc cgggagaccg cgugccucgc caagagcuac     2280

gcccagaugu ggcagcugcu guacuuccac cgccgggacc uccgccugau ggccaacgcg     2340

aucugcucca gcgugcccgu cgacugggug cccaccggcc ggacgaccug gagcauccac     2400

gggaagggcg aguggaugac caccgaggac augcuggugg ucuggaaccg cguguggauc     2460

gaggagaacg accacaugga ggacaagacg ccggugacca aguggaccga cauccccuac     2520

cucgggaagc gggaggaccu guggugcggc ucccugaucg gccaccgccc ccggaccacg     2580

ugggccgaga acaucaagaa caccgucaac auggugcgcc ggaucaucgg ggacgaggag     2640

aaguacaugg acuaccucag cacccaggug cgcuaccugg gcgaggaggg guccaccccc     2700

ggcguccug                                                             2709


<210>  141
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS mRNA GC

<400>  141
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cuccgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca cgugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accacgagca ccugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacgcgca agcugcagac ccggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacg      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac cuggguggac      660

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      720

aucgagcucg ugacgaccac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacacgca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca cgggccacga gaccgacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggacgggcc uggacuucag cgaccuguac     1200

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg cucccaggag     1380

ggggccgugc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacgcuc     1560

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1620

gcccagaugg ccguggacau gcagacgcug acccccgucg ggcgccugau caccgcgaac     1680

cccgugauca ccgagagcac ggagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacccacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1860

gugcuggggg acacggcgug ggacuucggc agcgucggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacccugcug auguggcucg ggcugaacgc caagaacggc     2040

uccaucagcc ugaugugccu cgcgcugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  142
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS mRNA GC

<400>  142
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca cgugcgacgc caccaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca cguggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac gcgcagccag      420

accuggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac gugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gacggacgag     1080

aaccgcgcga aggucgagau caccccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacaccggc accccgcacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acacggcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acgcccgugg ggcggcugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgccguuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacgcacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cucccucggg     1920

aaggggaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacgcuccug auguggcugg gccucaacgc caagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccguc     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  143
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS mRNA GC

<400>  143
agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc       60

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac cuggguggac      120

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      180

aucgagcucg ugacgaccac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      300

gacaagcagu ccgacacgca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      420

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      480

guccacggca gccagcacuc cgggaugauc gugaacgaca cgggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      600

uucggcuccc uggggcucga cugcgagccc cggacgggcc uggacuucag cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg      720

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg cucccaggag      840

ggggccgugc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc      900

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg      960

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacgcuc     1020

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1080

gcccagaugg ccguggacau gcagacgcug acccccgucg ggcgccugau caccgcgaac     1140

cccgugauca ccgagagcac ggagaacagc aagaugaugc ucgagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacccacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1320

gugcuggggg acacggcgug ggacuucggc agc                                  1353


<210>  144
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS mRNA GC

<400>  144
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc gacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcucuucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcugggcggg      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

ccggugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  145
<211>  10257
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein CDS mRNA GC

<400>  145
augaagaacc ccaaggagga gauccgccgg auccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg ugaacccgcu cgggggccug aagcggcugc ccgccgggcu ccugcugggc      120

cacgggccca uccgcauggu gcucgcgauc cuggccuucc ugcgguucac cgccaucaag      180

cccagccucg gccugaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcuccgga ucaucaacgc ccgcaaggag      300

cggaagcgcc ggggcgccga caccagcauc gggaucaucg gccugcugcu cacgaccgcc      360

auggcggccg agaucacccg ccgggggucc gccuacuaca uguaccugga ccgcagcgac      420

gccggcaagg ccaucuccuu cgcgaccacg cugggcguga acaagugcca cgugcagauc      480

auggaccucg ggcacaugug cgacgccacc augagcuacg agugcccgau gcuggacgag      540

ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca ccuccacgug gguggucuac      600

gggaccugcc accacaagaa gggcgaggcc cggcgcagcc ggcgcgccgu gacccugccc      660

ucccacagca cccggaagcu ccagacgcgc ucccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugaucaaggu ggagaacugg aucuuccgga accccggcuu cgcccucguc      780

gcgguggcca ucgccuggcu gcuggggucc agcaccuccc agaaggugau cuaccucguc      840

augauccugc ugaucgcccc ggccuacagc auccgcugca ucggcguguc caaccgggac      900

uucguggagg ggaugagcgg cgggacgugg gucgacgugg ugcucgagca cggcggcugc      960

gucaccguga uggcgcagga caagcccacc guggacaucg agcuggucac cacgaccgug     1020

uccaacaugg ccgagguccg cagcuacugc uacgaggcca gcaucuccga cauggccagc     1080

gacucccggu gccccaccca gggggaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacgcucgu ggaccggggc ugggggaacg gcugcgggcu guucggcaag     1200

ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga agaugaccgg gaaguccauc     1260

cagcccgaga accucgagua ccgcaucaug cugagcgugc acggcuccca gcacagcggg     1320

augaucggcu acgagacgga cgaggaccgg gccaaggugg aggucacccc gaacuccccg     1380

cgcgccgagg ccacccuggg cggguucggc agccucgggc uggacugcga gccccggacc     1440

ggccuggacu ucuccgaccu cuacuaccug acgaugaaca acaagcacug gcuggugcac     1500

aaggaguggu uccacgacau cccccucccg uggcacgcgg gggccgacac cggcaccccg     1560

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1620

gucguggugc ugggcagcca ggagggggcg guccacacgg cccuggccgg cgcccucgag     1680

gccgagaugg acggggcgaa gggccggcug uucuccgggc accugaagug ccgccucaag     1740

auggacaagc ugcggcugaa gggcgugagc uacucccucu gcaccgccgc cuucaccuuc     1800

accaaggugc ccgccgagac ccugcacggc acggucaccg uggaggugca guacgccggg     1860

accgacggcc ccugcaagau cccgguccag auggcggugg acaugcagac ccugacgccc     1920

guggggcgcc ucaucaccgc caaccccguc aucaccgaga gcaccgagaa cuccaagaug     1980

augcuggagc uggacccgcc guucggcgac agcuacaucg ugaucggggu gggcgacaag     2040

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     2100

guccgcggcg cgaagcggau ggccgugcuc ggggacaccg ccugggacuu cggcagcgug     2160

ggcggcgucu ucaacucccu ggggaagggg auccaccaga ucuucggcgc cgccuucaag     2220

agccuguucg ggggcauguc cugguucagc cagauccuca ucgggacgcu gcuggugugg     2280

cucggccuga acaccaagaa cggcuccauc agccugaccu gccucgcgcu gggcggcgug     2340

augaucuucc uguccaccgc cgucagcgcc gacguggggu gcuccgugga cuucagcaag     2400

aaggagacgc gcugcgggac cggcgucuuc aucuacaacg acguggaggc cuggcgcgac     2460

cgguacaagu accaccccga cuccccgcgc cggcucgccg cggccgugaa gcaggccugg     2520

gaggagggga ucugcggcau cagcuccguc agccgcaugg agaacaucau guggaagucc     2580

guggaggggg agcugaacgc cauccuggag gagaacggcg ugcagcucac cgucguggug     2640

gggagcguca agaaccccau guggcggggc ccgcagcgcc ugcccgugcc cgugaacgag     2700

cugccgcacg gcuggaaggc cugggggaag agcuacuucg uccgggcggc caagaccaac     2760

aacuccuucg ugguggacgg cgacacgcuc aaggagugcc cccuggagca ccgcgccugg     2820

aacagcuucc uggucgagga ccacggguuc ggcguguucc acaccuccgu guggcucaag     2880

guccgggagg acuacagccu ggagugcgac cccgccguga ucgggaccgc ggucaagggc     2940

cgcgaggccg cccacuccga ccugggcuac uggaucgaga gcgagaagaa cgacaccugg     3000

cggcucaagc gcgcccaccu gaucgagaug aagacgugcg aguggcccaa gucccacacc     3060

cuguggaccg acggggugga ggagagcgac cucaucaucc cgaagucccu ggccggcccc     3120

cugagccacc acaacacccg ggagggguac cgcacccagg ugaagggccc cuggcacucc     3180

gaggagcucg agauccgguu cgaggagugc cccgggacca aggucuacgu ggaggagacc     3240

ugcggcaccc gcggcccgag ccugcggucc acgaccgcga gcgggcgcgu gaucgaggag     3300

uggugcugcc gggagugcac caugccgccc cuguccuucc gcgccaagga cggcugcugg     3360

uacgggaugg agauccggcc ccgcaaggag ccggagagca accucguccg cuccauggug     3420

accgccggca gcacggacca cauggaccac uucucccugg gcgugcuggu cauccuccug     3480

auggugcagg aggggcugaa gaagcggaug accaccaaga ucaucaugag caccagcaug     3540

gccgugcucg ucgugaugau ccugggcggg uucuccauga gcgaccuggc caagcucgug     3600

auccugaugg gcgcgaccuu cgccgagaug aacacgggcg gcgacgucgc ccaccuggcc     3660

cucguggccg cguucaaggu gcgccccgcc cugcuggucu ccuucaucuu ccgggccaac     3720

uggaccccgc gcgagagcau gcuccuggcc cuggccuccu gccuccugca gaccgcgauc     3780

agcgcccugg agggggaccu cauggugcug aucaacgggu ucgcccuggc cuggcucgcg     3840

auccgggcca uggccgugcc ccgcaccgac aacaucgccc ugccgauccu ggccgcgcuc     3900

acgccccugg cccggggcac ccugcucguc gccuggcgcg ccgggcuggc cacgugcggc     3960

gggaucaugc ugcucucccu gaagggcaag ggcagcguga agaagaaccu gcccuucgug     4020

auggcgcucg ggcugaccgc cguccgggug guggacccca ucaacgucgu gggccugcuc     4080

cugcugacgc gcuccgggaa gcggagcugg ccgcccuccg aggugcucac cgccgucggc     4140

cugaucugcg cccuggccgg gggcuucgcg aaggccgaca ucgagauggc cggccccaug     4200

gccgcggugg ggcuccugau cgugagcuac gucguguccg gcaagagcgu ggacauguac     4260

aucgagcgcg ccggggacau caccugggag aaggacgccg aggucaccgg caacuccccg     4320

cggcuggacg uggcccucga cgagagcggg gacuucuccc ugguggagga ggacggcccg     4380

cccaugcgcg agaucauccu gaaggucgug cucauggcca ucugcggcau gaaccccauc     4440

gcgaucccgu ucgccgccgg ggccugguac guguacguca agacgggcaa gcggagcggg     4500

gcccuguggg acgugcccgc gcccaaggag gugaagaagg gcgagaccac cgacggcguc     4560

uaccgcguga ugacccggcg ccugcugggg uccacccagg ugggcgucgg ggugaugcag     4620

gagggcgugu uccacaccau guggcacguc accaaggggg ccgcccuccg gagcggcgag     4680

ggccgccugg accccuacug gggcgacgug aagcaggacc uggucagcua cugcgggccg     4740

uggaagcucg acgccgccug ggacgggcug uccgaggugc agcugcucgc ggugccgccc     4800

ggcgagcgcg cccggaacau ccagacccug cccgggaucu ucaagacgaa ggacggcgac     4860

aucggcgccg ucgcccugga cuacccggcg gggaccagcg gcucccccau ccucgacaag     4920

ugcgggcgcg ugaucggccu guacgggaac ggcgugguca ucaagaacgg cagcuacgug     4980

uccgccauca cccaggggaa gcgggaggag gagacccccg uggagugcuu cgagcccagc     5040

augcugaaga agaagcagcu cacgguccug gaccugcacc cgggcgccgg gaagacccgc     5100

cgggugcucc ccgagaucgu gcgcgaggcc aucaagaagc ggcugcgcac cgucauccug     5160

gcccccaccc gggugguggc ggccgagaug gaggaggccc uccgcggccu gcccguccgg     5220

uacaugacga ccgccgugaa cgugacccac uccgggaccg agaucgucga ccugaugugc     5280

cacgccacgu ucaccagccg ccuccugcag ccgauccggg ugcccaacua caaccugaac     5340

aucauggacg aggcgcacuu caccgacccc uccagcaucg ccgcccgcgg cuacaucucc     5400

acccgggugg agaugggcga ggccgccgcg aucuucauga cggccacccc gccggggacc     5460

cgcgacgccu uccccgacag caacuccccc aucauggaca ccgaggucga ggugcccgag     5520

cgggccugga gcuccggcuu cgacugggug accgaccaca gcgggaagac ggucugguuc     5580

gugccguccg ugcgcaacgg caacgagauc gccgcgugcc ucaccaaggc cggcaagcgg     5640

gucauccagc ugagccgcaa gaccuucgag accgaguucc agaagacgaa gaaccaggag     5700

ugggacuucg ugaucaccac cgacaucucc gagauggggg ccaacuucaa ggccgaccgg     5760

gugaucgaca gccgccgcug ccugaagccc gucauccucg acggcgagcg ggugauccug     5820

gcggggccca ugcccgugac ccacgccagc gccgcccagc gccggggccg caucgggcgg     5880

aacccgaaca agcccggcga cgaguacaug uacggcgggg gcugcgccga gacggacgag     5940

gggcacgcgc acuggcugga ggcccgcaug cuccuggaca acaucuaccu gcaggacggc     6000

cucaucgccu cccuguaccg gcccgaggcc gacaaggucg ccgcgaucga gggggaguuc     6060

aagcugcgca ccgagcagcg gaagaccuuc guggagcuca ugaagcgcgg cgaccugccg     6120

guguggcugg ccuaccaggu cgccagcgcc ggcaucaccu acacggaccg gcgcuggugc     6180

uucgacggga ccaccaacaa caccaucaug gaggacuccg ugcccgccga gguguggacg     6240

aaguacggcg agaagcgggu ccucaagccc cgcuggaugg acgcgcgggu gugcagcgac     6300

cacgccgccc ugaaguccuu caaggaguuc gccgcgggga agcgcggcgc cgcccugggg     6360

gugauggagg cccucggcac ccugcccggc cacaugaccg agcgguucca ggaggccauc     6420

gacaaccugg cgguccucau gcgcgccgag accgggagcc ggccguacaa ggccgccgcc     6480

gcgcagcugc ccgagacgcu ggagaccauc augcuccugg gccugcucgg gaccgugucc     6540

cugggcaucu ucuucgugcu gaugcgcaac aagggcaucg ggaagauggg cuucgggaug     6600

gucacccucg gcgccagcgc cuggcugaug uggcuguccg agaucgagcc cgcccgcauc     6660

gccugcgugc ucaucguggu cuuccugcug cucguggugc ugauccccga gccggagaag     6720

cagcggagcc cccaggacaa ccagauggcg aucaucauca uggucgccgu ggggcugcuc     6780

ggccugauca cggccaacga gcugggcugg cucgagcgca ccaagaacga caucgcccac     6840

cugauggggc ggcgcgagga gggcgcgacc augggguucu ccauggacau cgaccugcgg     6900

cccgccagcg ccugggccau cuacgccgcg cucaccacgc ugaucacccc cgccguccag     6960

cacgccguga ccaccuccua caacaacuac agccugaugg ccauggccac gcaggcgggc     7020

gugcucuucg ggaugggcaa gggcaugccg uucaugcacg gggaccuggg cgucccgcug     7080

cucaugaugg ggugcuacuc ccagcugacc ccgcugaccc ucaucguggc caucauccug     7140

cugguggccc acuacaugua ccucaucccc ggccugcagg ccgccgcggc ccgcgccgcc     7200

cagaagcgga ccgccgcggg gaucaugaag aacccggucg uggacggcau cguggucacg     7260

gacaucgaca ccaugaccau cgacccccag guggagaaga agaugggcca ggugcugcuc     7320

aucgccgucg ccaucagcuc cgccgugcug cugcgcaccg cgugggggug gggcgaggcc     7380

ggggcccuca ucacggccgc caccagcacc cugugggagg gcucccccaa caaguacugg     7440

aacagcagca ccgcgaccuc ccugugcaac aucuuccggg ggagcuaccu cgccggcgcc     7500

ucccugaucu acacggugac ccgcaacgcc ggccugguca agcggcgcgg gggcgggacc     7560

ggcgagaccc ucggcgagaa guggaaggcc cggcugaacc agaugagcgc gcuggaguuc     7620

uacuccuaca agaagagcgg gaucacggag gugugccgcg aggaggcccg gcgcgcccuc     7680

aaggacggcg uggccaccgg gggccacgcc gucucccggg ggagcgcgaa gauccgcugg     7740

cuggaggagc ggggcuaccu gcagcccuac ggcaaggugg uggaccuggg gugcggccgc     7800

gggggcuggu ccuacuacgc cgccaccauc cggaaggucc aggaggugcg cggguacacc     7860

aagggcggcc cggggcacga ggagcccaug cucgugcaga gcuacggcug gaacaucguc     7920

cgccugaagu ccggggugga cguguuccac auggccgcgg agcccugcga cacgcugcuc     7980

ugcgacaucg gcgagagcuc cagcuccccc gaggucgagg agacccggac ccugcgcgug     8040

cugagcaugg ugggggacug gcucgagaag cggccgggcg ccuucugcau caagguccug     8100

ugccccuaca ccuccacgau gauggagacc auggagcgcc ugcagcggcg ccacggcggg     8160

ggccucgugc gggugccccu gugccgcaac agcacccacg agauguacug ggucuccggg     8220

gccaagagca acaucaucaa gagcgugucc accacgagcc agcugcuccu gggccggaug     8280

gacggccccc gccggccggu gaaguacgag gaggacguca accugggguc cggcacccgc     8340

gccguggcca gcugcgcgga ggcccccaac augaagauca ucgggcggcg caucgagcgg     8400

auccgcaacg agcacgccga gaccugguuc cucgacgaga accaccccua ccggaccugg     8460

gccuaccacg gcuccuacga ggccccgacg caggggagcg cguccagccu ggugaacggc     8520

gucgugcgcc ugcucuccaa gcccugggac guggucaccg gcgugaccgg gaucgccaug     8580

accgacacga cccccuacgg ccagcagcgg guguucaagg agaaggucga cacccgcgug     8640

cccgacccgc aggaggggac ccgccaggug augaacaucg ucagcuccug gcuguggaag     8700

gagcugggca agcggaagcg cccccgggug ugcacgaagg aggaguucau caacaaggug     8760

cgcagcaacg ccgcccucgg ggccaucuuc gaggaggaga aggaguggaa gaccgcgguc     8820

gaggccguga acgacccccg guucugggcc cuggucgacc gcgagcggga gcaccaccuc     8880

cgcggcgagu gccacuccug cguguacaac augaugggca agcgggagaa gaagcagggg     8940

gaguucggca aggccaaggg gagccgcgcc aucugguaca uguggcucgg cgcgcgguuc     9000

cuggaguucg aggcccuggg guuccucaac gaggaccacu ggaugggccg cgagaacucc     9060

ggcgggggcg uggaggggcu gggccugcag cggcucgggu acauccugga ggagaugaac     9120

cgcgcccccg gcggcaagau guacgccgac gacaccgcgg ggugggacac ccggaucagc     9180

aaguucgacc uggagaacga ggcccucauc acgaaccaga uggaggaggg ccaccgcacc     9240

cuggcccugg ccgucaucaa guacaccuac cagaacaagg uggugaaggu ccuccggccg     9300

gccgagggcg ggaagaccgu gauggacauc aucucccgcc aggaccagcg gggcagcggc     9360

caggugguca cguacgcgcu gaacaccuuc accaaccugg uggugcagcu cauccgcaac     9420

auggaggccg aggagguccu ggagaugcag gaccuguggc uccugcggaa gcccgagaag     9480

gugacccgcu ggcugcagag caacggcugg gaccgccuca agcggauggc cguguccggg     9540

gacgacugcg ucgugaagcc caucgacgac cgcuucgccc acgcccugcg guuccugaac     9600

gacaugggca aggugcgcaa ggacacccag gaguggaagc ccagcacggg gugguccaac     9660

ugggaggagg ucccguucug cagccaccac uucaacaagc ucuaccugaa ggacggccgg     9720

uccaucgugg ugcccugccg ccaccaggac gagcugaucg gccgggcgcg cgucagcccc     9780

ggggccggcu gguccauccg ggagaccgcc ugccucgcca agagcuacgc ccagaugugg     9840

cagcugcugu acuuccaccg ccgggaccuc cgccugaugg cgaacgccau cugcuccgcc     9900

gugcccgugg acuggguccc gaccgggcgg accacgugga gcauccacgg caagggggag     9960

uggaugacca ccgaggacau gcugauggug uggaaccgcg uguggaucga ggagaacgac    10020

cacauggagg acaagacccc cgucacgaag uggaccgaca uccccuaccu cggcaagcgg    10080

gaggaccugu ggugcggcuc ccugaucggg caccgccccc ggaccaccug ggccgagaac    10140

aucaaggaca cggugaacau ggugcgccgg aucaucggcg acgaggagaa guacauggac    10200

uaccucagca cccagguccg cuaccugggg gaggagggcu ccaccccggg ggugcug       10257


<210>  146
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C CDS mRNA GC

<400>  146
augaagaacc ccaaggagga gauccgccgg auccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg ugaacccgcu cgggggccug aagcggcugc ccgccgggcu ccugcugggc      120

cacgggccca uccgcauggu gcucgcgauc cuggccuucc ugcgguucac cgccaucaag      180

cccagccucg gccugaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcuccgga ucaucaacgc ccgcaaggag      300

cggaagcgcc gg                                                          312


<210>  147
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-ER anchor CDS mRNA GC

<400>  147
augaagaacc ccaaggagga gauccgccgg auccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg ugaacccgcu cgggggccug aagcggcugc ccgccgggcu ccugcugggc      120

cacgggccca uccgcauggu gcucgcgauc cuggccuucc ugcgguucac cgccaucaag      180

cccagccucg gccugaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcuccgga ucaucaacgc ccgcaaggag      300

cggaagcgcc ggggcgccga caccagcauc gggaucaucg gccugcugcu cacgaccgcc      360

auggcg                                                                 366


<210>  148
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) pr CDS mRNA GC

<400>  148
gccgagauca cccgccgggg guccgccuac uacauguacc uggaccgcag cgacgccggc       60

aaggccaucu ccuucgcgac cacgcugggc gugaacaagu gccacgugca gaucauggac      120

cucgggcaca ugugcgacgc caccaugagc uacgagugcc cgaugcugga cgagggcguc      180

gagcccgacg acguggacug cuggugcaac accaccucca cguggguggu cuacgggacc      240

ugccaccaca agaagggcga ggcccggcgc agccggcgc                             279


<210>  149
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) M CDS mRNA GC

<400>  149
gccgugaccc ugcccuccca cagcacccgg aagcuccaga cgcgcuccca gaccuggcug       60

gagagccgcg aguacaccaa gcaccugauc aagguggaga acuggaucuu ccggaacccc      120

ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg gguccagcac cucccagaag      180

gugaucuacc ucgucaugau ccugcugauc gccccggccu acagc                      225


<210>  150
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prM CDS mRNA GC

<400>  150
gccgagauca cccgccgggg guccgccuac uacauguacc uggaccgcag cgacgccggc       60

aaggccaucu ccuucgcgac cacgcugggc gugaacaagu gccacgugca gaucauggac      120

cucgggcaca ugugcgacgc caccaugagc uacgagugcc cgaugcugga cgagggcguc      180

gagcccgacg acguggacug cuggugcaac accaccucca cguggguggu cuacgggacc      240

ugccaccaca agaagggcga ggcccggcgc agccggcgcg ccgugacccu gcccucccac      300

agcacccgga agcuccagac gcgcucccag accuggcugg agagccgcga guacaccaag      360

caccugauca agguggagaa cuggaucuuc cggaaccccg gcuucgcccu cgucgcggug      420

gccaucgccu ggcugcuggg guccagcacc ucccagaagg ugaucuaccu cgucaugauc      480

cugcugaucg ccccggccua cagc                                             504


<210>  151
<211>  1500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) E CDS mRNA GC

<400>  151
auccgcugca ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg       60

gucgacgugg ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc      120

guggacaucg agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc      180

uacgaggcca gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc      240

uaccuggaca agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc      300

ugggggaacg gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg      360

ugcagcaaga agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug      420

cugagcgugc acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg      480

gccaaggugg aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc      540

agccucgggc uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug      600

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg      660

uggcacgcgg gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag      720

uucaaggacg cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg      780

guccacacgg cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug      840

uucuccgggc accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc      900

uacucccucu gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc      960

acggucaccg uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag     1020

auggcggugg acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc     1080

aucaccgaga gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac     1140

agcuacaucg ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc     1200

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc     1260

ggggacaccg ccugggacuu cggcagcgug ggcggcgucu ucaacucccu ggggaagggg     1320

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1380

cagauccuca ucgggacgcu gcuggugugg cucggccuga acaccaagaa cggcuccauc     1440

agccugaccu gccucgcgcu gggcggcgug augaucuucc uguccaccgc cgucagcgcc     1500


<210>  152
<211>  2004
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME CDS mRNA GC

<400>  152
gccgagauca cccgccgggg guccgccuac uacauguacc uggaccgcag cgacgccggc       60

aaggccaucu ccuucgcgac cacgcugggc gugaacaagu gccacgugca gaucauggac      120

cucgggcaca ugugcgacgc caccaugagc uacgagugcc cgaugcugga cgagggcguc      180

gagcccgacg acguggacug cuggugcaac accaccucca cguggguggu cuacgggacc      240

ugccaccaca agaagggcga ggcccggcgc agccggcgcg ccgugacccu gcccucccac      300

agcacccgga agcuccagac gcgcucccag accuggcugg agagccgcga guacaccaag      360

caccugauca agguggagaa cuggaucuuc cggaaccccg gcuucgcccu cgucgcggug      420

gccaucgccu ggcugcuggg guccagcacc ucccagaagg ugaucuaccu cgucaugauc      480

cugcugaucg ccccggccua cagcauccgc ugcaucggcg uguccaaccg ggacuucgug      540

gaggggauga gcggcgggac gugggucgac guggugcucg agcacggcgg cugcgucacc      600

gugauggcgc aggacaagcc caccguggac aucgagcugg ucaccacgac cguguccaac      660

auggccgagg uccgcagcua cugcuacgag gccagcaucu ccgacauggc cagcgacucc      720

cggugcccca cccaggggga ggccuaccug gacaagcaga gcgacaccca guacgugugc      780

aagcgcacgc ucguggaccg gggcuggggg aacggcugcg ggcuguucgg caagggcucc      840

cuggucacgu gcgcgaaguu cacgugcagc aagaagauga ccgggaaguc cauccagccc      900

gagaaccucg aguaccgcau caugcugagc gugcacggcu cccagcacag cgggaugauc      960

ggcuacgaga cggacgagga ccgggccaag guggagguca ccccgaacuc cccgcgcgcc     1020

gaggccaccc ugggcggguu cggcagccuc gggcuggacu gcgagccccg gaccggccug     1080

gacuucuccg accucuacua ccugacgaug aacaacaagc acuggcuggu gcacaaggag     1140

ugguuccacg acaucccccu cccguggcac gcgggggccg acaccggcac cccgcacugg     1200

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug     1260

gugcugggca gccaggaggg ggcgguccac acggcccugg ccggcgcccu cgaggccgag     1320

auggacgggg cgaagggccg gcuguucucc gggcaccuga agugccgccu caagauggac     1380

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac cuucaccaag     1440

gugcccgccg agacccugca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1500

ggccccugca agaucccggu ccagauggcg guggacaugc agacccugac gcccgugggg     1560

cgccucauca ccgccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1620

gagcuggacc cgccguucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1680

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1740

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcag cgugggcggc     1800

gucuucaacu cccuggggaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1860

uucgggggca uguccugguu cagccagauc cucaucggga cgcugcuggu guggcucggc     1920

cugaacacca agaacggcuc caucagccug accugccucg cgcugggcgg cgugaugauc     1980

uuccugucca ccgccgucag cgcc                                            2004


<210>  153
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS1 CDS mRNA GC

<400>  153
gacguggggu gcuccgugga cuucagcaag aaggagacgc gcugcgggac cggcgucuuc       60

aucuacaacg acguggaggc cuggcgcgac cgguacaagu accaccccga cuccccgcgc      120

cggcucgccg cggccgugaa gcaggccugg gaggagggga ucugcggcau cagcuccguc      180

agccgcaugg agaacaucau guggaagucc guggaggggg agcugaacgc cauccuggag      240

gagaacggcg ugcagcucac cgucguggug gggagcguca agaaccccau guggcggggc      300

ccgcagcgcc ugcccgugcc cgugaacgag cugccgcacg gcuggaaggc cugggggaag      360

agcuacuucg uccgggcggc caagaccaac aacuccuucg ugguggacgg cgacacgcuc      420

aaggagugcc cccuggagca ccgcgccugg aacagcuucc uggucgagga ccacggguuc      480

ggcguguucc acaccuccgu guggcucaag guccgggagg acuacagccu ggagugcgac      540

cccgccguga ucgggaccgc ggucaagggc cgcgaggccg cccacuccga ccugggcuac      600

uggaucgaga gcgagaagaa cgacaccugg cggcucaagc gcgcccaccu gaucgagaug      660

aagacgugcg aguggcccaa gucccacacc cuguggaccg acggggugga ggagagcgac      720

cucaucaucc cgaagucccu ggccggcccc cugagccacc acaacacccg ggagggguac      780

cgcacccagg ugaagggccc cuggcacucc gaggagcucg agauccgguu cgaggagugc      840

cccgggacca aggucuacgu ggaggagacc ugcggcaccc gcggcccgag ccugcggucc      900

acgaccgcga gcgggcgcgu gaucgaggag uggugcugcc gggagugcac caugccgccc      960

cuguccuucc gcgccaagga cggcugcugg uacgggaugg agauccggcc ccgcaaggag     1020

ccggagagca accucguccg cuccauggug accgcc                               1056


<210>  154
<211>  675
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS2A CDS mRNA GC

<400>  154
ggcagcacgg accacaugga ccacuucucc cugggcgugc uggucauccu ccugauggug       60

caggaggggc ugaagaagcg gaugaccacc aagaucauca ugagcaccag cauggccgug      120

cucgucguga ugauccuggg cggguucucc augagcgacc uggccaagcu cgugauccug      180

augggcgcga ccuucgccga gaugaacacg ggcggcgacg ucgcccaccu ggcccucgug      240

gccgcguuca aggugcgccc cgcccugcug gucuccuuca ucuuccgggc caacuggacc      300

ccgcgcgaga gcaugcuccu ggcccuggcc uccugccucc ugcagaccgc gaucagcgcc      360

cuggaggggg accucauggu gcugaucaac ggguucgccc uggccuggcu cgcgauccgg      420

gccauggccg ugccccgcac cgacaacauc gcccugccga uccuggccgc gcucacgccc      480

cuggcccggg gcacccugcu cgucgccugg cgcgccgggc uggccacgug cggcgggauc      540

augcugcucu cccugaaggg caagggcagc gugaagaaga accugcccuu cgugauggcg      600

cucgggcuga ccgccguccg ggugguggac cccaucaacg ucgugggccu gcuccugcug      660

acgcgcuccg ggaag                                                       675


<210>  155
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS2B CDS mRNA GC

<400>  155
agcuggccgc ccuccgaggu gcucaccgcc gucggccuga ucugcgcccu ggccgggggc       60

uucgcgaagg ccgacaucga gauggccggc cccauggccg cgguggggcu ccugaucgug      120

agcuacgucg uguccggcaa gagcguggac auguacaucg agcgcgccgg ggacaucacc      180

ugggagaagg acgccgaggu caccggcaac uccccgcggc uggacguggc ccucgacgag      240

agcggggacu ucucccuggu ggaggaggac ggcccgccca ugcgcgagau cauccugaag      300

gucgugcuca uggccaucug cggcaugaac cccaucgcga ucccguucgc cgccggggcc      360

ugguacgugu acgucaagac gggcaagcgg                                       390


<210>  156
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS3 CDS mRNA GC

<400>  156
agcggggccc ugugggacgu gcccgcgccc aaggagguga agaagggcga gaccaccgac       60

ggcgucuacc gcgugaugac ccggcgccug cuggggucca cccagguggg cgucggggug      120

augcaggagg gcguguucca caccaugugg cacgucacca agggggccgc ccuccggagc      180

ggcgagggcc gccuggaccc cuacuggggc gacgugaagc aggaccuggu cagcuacugc      240

gggccgugga agcucgacgc cgccugggac gggcuguccg aggugcagcu gcucgcggug      300

ccgcccggcg agcgcgcccg gaacauccag acccugcccg ggaucuucaa gacgaaggac      360

ggcgacaucg gcgccgucgc ccuggacuac ccggcgggga ccagcggcuc ccccauccuc      420

gacaagugcg ggcgcgugau cggccuguac gggaacggcg uggucaucaa gaacggcagc      480

uacguguccg ccaucaccca ggggaagcgg gaggaggaga cccccgugga gugcuucgag      540

cccagcaugc ugaagaagaa gcagcucacg guccuggacc ugcacccggg cgccgggaag      600

acccgccggg ugcuccccga gaucgugcgc gaggccauca agaagcggcu gcgcaccguc      660

auccuggccc ccacccgggu gguggcggcc gagauggagg aggcccuccg cggccugccc      720

guccgguaca ugacgaccgc cgugaacgug acccacuccg ggaccgagau cgucgaccug      780

augugccacg ccacguucac cagccgccuc cugcagccga uccgggugcc caacuacaac      840

cugaacauca uggacgaggc gcacuucacc gaccccucca gcaucgccgc ccgcggcuac      900

aucuccaccc ggguggagau gggcgaggcc gccgcgaucu ucaugacggc caccccgccg      960

gggacccgcg acgccuuccc cgacagcaac ucccccauca uggacaccga ggucgaggug     1020

cccgagcggg ccuggagcuc cggcuucgac ugggugaccg accacagcgg gaagacgguc     1080

ugguucgugc cguccgugcg caacggcaac gagaucgccg cgugccucac caaggccggc     1140

aagcggguca uccagcugag ccgcaagacc uucgagaccg aguuccagaa gacgaagaac     1200

caggaguggg acuucgugau caccaccgac aucuccgaga ugggggccaa cuucaaggcc     1260

gaccggguga ucgacagccg ccgcugccug aagcccguca uccucgacgg cgagcgggug     1320

auccuggcgg ggcccaugcc cgugacccac gccagcgccg cccagcgccg gggccgcauc     1380

gggcggaacc cgaacaagcc cggcgacgag uacauguacg gcgggggcug cgccgagacg     1440

gacgaggggc acgcgcacug gcuggaggcc cgcaugcucc uggacaacau cuaccugcag     1500

gacggccuca ucgccucccu guaccggccc gaggccgaca aggucgccgc gaucgagggg     1560

gaguucaagc ugcgcaccga gcagcggaag accuucgugg agcucaugaa gcgcggcgac     1620

cugccggugu ggcuggccua ccaggucgcc agcgccggca ucaccuacac ggaccggcgc     1680

uggugcuucg acgggaccac caacaacacc aucauggagg acuccgugcc cgccgaggug     1740

uggacgaagu acggcgagaa gcggguccuc aagccccgcu ggauggacgc gcgggugugc     1800

agcgaccacg ccgcccugaa guccuucaag gaguucgccg cggggaagcg c              1851


<210>  157
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS4A CDS mRNA GC

<400>  157
ggcgccgccc ugggggugau ggaggcccuc ggcacccugc ccggccacau gaccgagcgg       60

uuccaggagg ccaucgacaa ccuggcgguc cucaugcgcg ccgagaccgg gagccggccg      120

uacaaggccg ccgccgcgca gcugcccgag acgcuggaga ccaucaugcu ccugggccug      180

cucgggaccg ugucccuggg caucuucuuc gugcugaugc gcaacaaggg caucgggaag      240

augggcuucg ggauggucac ccucggcgcc agcgccuggc ugauguggcu guccgagauc      300

gagcccgccc gcaucgccug cgugcucauc guggucuucc ugcugcucgu ggugcugauc      360

cccgagccgg agaagcagcg gagcccccag gacaaccaga uggcgaucau caucaugguc      420

gccguggggc ugcucggccu gaucacggcc                                       450


<210>  158
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS4B CDS mRNA GC

<400>  158
aacgagcugg gcuggcucga gcgcaccaag aacgacaucg cccaccugau ggggcggcgc       60

gaggagggcg cgaccauggg guucuccaug gacaucgacc ugcggcccgc cagcgccugg      120

gccaucuacg ccgcgcucac cacgcugauc acccccgccg uccagcacgc cgugaccacc      180

uccuacaaca acuacagccu gauggccaug gccacgcagg cgggcgugcu cuucgggaug      240

ggcaagggca ugccguucau gcacggggac cugggcgucc cgcugcucau gauggggugc      300

uacucccagc ugaccccgcu gacccucauc guggccauca uccugcuggu ggcccacuac      360

auguaccuca uccccggccu gcaggccgcc gcggcccgcg ccgcccagaa gcggaccgcc      420

gcggggauca ugaagaaccc ggucguggac ggcaucgugg ucacggacau cgacaccaug      480

accaucgacc cccaggugga gaagaagaug ggccaggugc ugcucaucgc cgucgccauc      540

agcuccgccg ugcugcugcg caccgcgugg ggguggggcg aggccggggc ccucaucacg      600

gccgccacca gcacccugug ggagggcucc cccaacaagu acuggaacag cagcaccgcg      660

accucccugu gcaacaucuu ccgggggagc uaccucgccg gcgccucccu gaucuacacg      720

gugacccgca acgccggccu ggucaagcgg cgc                                   753


<210>  159
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS5 CDS mRNA GC

<400>  159
gggggcggga ccggcgagac ccucggcgag aaguggaagg cccggcugaa ccagaugagc       60

gcgcuggagu ucuacuccua caagaagagc gggaucacgg aggugugccg cgaggaggcc      120

cggcgcgccc ucaaggacgg cguggccacc gggggccacg ccgucucccg ggggagcgcg      180

aagauccgcu ggcuggagga gcggggcuac cugcagcccu acggcaaggu gguggaccug      240

gggugcggcc gcgggggcug guccuacuac gccgccacca uccggaaggu ccaggaggug      300

cgcggguaca ccaagggcgg cccggggcac gaggagccca ugcucgugca gagcuacggc      360

uggaacaucg uccgccugaa guccggggug gacguguucc acauggccgc ggagcccugc      420

gacacgcugc ucugcgacau cggcgagagc uccagcuccc ccgaggucga ggagacccgg      480

acccugcgcg ugcugagcau ggugggggac uggcucgaga agcggccggg cgccuucugc      540

aucaaggucc ugugccccua caccuccacg augauggaga ccauggagcg ccugcagcgg      600

cgccacggcg ggggccucgu gcgggugccc cugugccgca acagcaccca cgagauguac      660

ugggucuccg gggccaagag caacaucauc aagagcgugu ccaccacgag ccagcugcuc      720

cugggccgga uggacggccc ccgccggccg gugaaguacg aggaggacgu caaccugggg      780

uccggcaccc gcgccguggc cagcugcgcg gaggccccca acaugaagau caucgggcgg      840

cgcaucgagc ggauccgcaa cgagcacgcc gagaccuggu uccucgacga gaaccacccc      900

uaccggaccu gggccuacca cggcuccuac gaggccccga cgcaggggag cgcguccagc      960

cuggugaacg gcgucgugcg ccugcucucc aagcccuggg acguggucac cggcgugacc     1020

gggaucgcca ugaccgacac gacccccuac ggccagcagc ggguguucaa ggagaagguc     1080

gacacccgcg ugcccgaccc gcaggagggg acccgccagg ugaugaacau cgucagcucc     1140

uggcugugga aggagcuggg caagcggaag cgcccccggg ugugcacgaa ggaggaguuc     1200

aucaacaagg ugcgcagcaa cgccgcccuc ggggccaucu ucgaggagga gaaggagugg     1260

aagaccgcgg ucgaggccgu gaacgacccc cgguucuggg cccuggucga ccgcgagcgg     1320

gagcaccacc uccgcggcga gugccacucc ugcguguaca acaugauggg caagcgggag     1380

aagaagcagg gggaguucgg caaggccaag gggagccgcg ccaucuggua cauguggcuc     1440

ggcgcgcggu uccuggaguu cgaggcccug ggguuccuca acgaggacca cuggaugggc     1500

cgcgagaacu ccggcggggg cguggagggg cugggccugc agcggcucgg guacauccug     1560

gaggagauga accgcgcccc cggcggcaag auguacgccg acgacaccgc ggggugggac     1620

acccggauca gcaaguucga ccuggagaac gaggcccuca ucacgaacca gauggaggag     1680

ggccaccgca cccuggcccu ggccgucauc aaguacaccu accagaacaa gguggugaag     1740

guccuccggc cggccgaggg cgggaagacc gugauggaca ucaucucccg ccaggaccag     1800

cggggcagcg gccagguggu cacguacgcg cugaacaccu ucaccaaccu gguggugcag     1860

cucauccgca acauggaggc cgaggagguc cuggagaugc aggaccugug gcuccugcgg     1920

aagcccgaga aggugacccg cuggcugcag agcaacggcu gggaccgccu caagcggaug     1980

gccguguccg gggacgacug cgucgugaag cccaucgacg accgcuucgc ccacgcccug     2040

cgguuccuga acgacauggg caaggugcgc aaggacaccc aggaguggaa gcccagcacg     2100

ggguggucca acugggagga ggucccguuc ugcagccacc acuucaacaa gcucuaccug     2160

aaggacggcc gguccaucgu ggugcccugc cgccaccagg acgagcugau cggccgggcg     2220

cgcgucagcc ccggggccgg cugguccauc cgggagaccg ccugccucgc caagagcuac     2280

gcccagaugu ggcagcugcu guacuuccac cgccgggacc uccgccugau ggcgaacgcc     2340

aucugcuccg ccgugcccgu ggacuggguc ccgaccgggc ggaccacgug gagcauccac     2400

ggcaaggggg aguggaugac caccgaggac augcugaugg uguggaaccg cguguggauc     2460

gaggagaacg accacaugga ggacaagacc cccgucacga aguggaccga cauccccuac     2520

cucggcaagc gggaggaccu guggugcggc ucccugaucg ggcaccgccc ccggaccacc     2580

ugggccgaga acaucaagga cacggugaac auggugcgcc ggaucaucgg cgacgaggag     2640

aaguacaugg acuaccucag cacccagguc cgcuaccugg gggaggaggg cuccaccccg     2700

ggggugcug                                                             2709


<210>  160
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS mRNA GC

<400>  160
cggaucauca acgcccgcaa ggagcggaag cgccggggcg ccgacaccag caucgggauc       60

aucggccugc ugcucacgac cgccauggcg gccgagauca cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc aaggccaucu ccuucgcgac cacgcugggc      180

gugaacaagu gccacgugca gaucauggac cucgggcaca ugugcgacgc caccaugagc      240

uacgagugcc cgaugcugga cgagggcguc gagcccgacg acguggacug cuggugcaac      300

accaccucca cguggguggu cuacgggacc ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgugacccu gcccucccac agcacccgga agcuccagac gcgcucccag      420

accuggcugg agagccgcga guacaccaag caccugauca agguggagaa cuggaucuuc      480

cggaaccccg gcuucgcccu cgucgcggug gccaucgccu ggcugcuggg guccagcacc      540

ucccagaagg ugaucuaccu cgucaugauc cugcugaucg ccccggccua cagcauccgc      600

ugcaucggcg uguccaaccg ggacuucgug gaggggauga gcggcgggac gugggucgac      660

guggugcucg agcacggcgg cugcgucacc gugauggcgc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccagcaucu ccgacauggc cagcgacucc cggugcccca cccaggggga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcacgc ucguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caagggcucc cuggucacgu gcgcgaaguu cacgugcagc      960

aagaagauga ccgggaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc ggcuacgaga cggacgagga ccgggccaag     1080

guggagguca ccccgaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccuc     1140

gggcuggacu gcgagccccg gaccggccug gacuucuccg accucuacua ccugacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu cccguggcac     1260

gcgggggccg acaccggcac cccgcacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagcgcca gaccgucgug gugcugggca gccaggaggg ggcgguccac     1380

acggcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggccg gcuguucucc     1440

gggcaccuga agugccgccu caagauggac aagcugcggc ugaagggcgu gagcuacucc     1500

cucugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggccccugca agaucccggu ccagauggcg     1620

guggacaugc agacccugac gcccgugggg cgccucauca ccgccaaccc cgucaucacc     1680

gagagcaccg agaacuccaa gaugaugcug gagcuggacc cgccguucgg cgacagcuac     1740

aucgugaucg gggugggcga caagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgcgaagc ggauggccgu gcucggggac     1860

accgccuggg acuucggcag cgugggcggc gucuucaacu cccuggggaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggga cgcugcuggu guggcucggc cugaacacca agaacggcuc caucagccug     2040

accugccucg cgcugggcgg cgugaugauc uuccugucca ccgccgucag cgccgacgug     2100

gggugcuccg uggacuucag caag                                            2124


<210>  161
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS mRNA GC

<400>  161
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagccggacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggugacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu gguggccgug gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ucaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucguc gagggcauga gcggcgggac cuggguggac      660

gugguccugg agcacggcgg gugcgugacg gugauggccc aggacaagcc gaccgucgac      720

aucgagcugg ugaccaccac gguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgucacgu gcgccaaguu caccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc ggcuacgaga ccgacgagga ccgcgccaag     1080

gucgagguga cgcccaacuc cccgcgggcc gaggcgaccc ucgggggcuu cgggagccug     1140

ggccuggacu gcgagccccg caccggccuc gacuucuccg accuguacua ccugaccaug     1200

aacaacaagc acuggcucgu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggggccg acacgggcac cccgcacugg aacaacaagg aggcccuggu cgaguucaag     1320

gacgcccacg cgaagcggca gaccguggug guccucggga gccaggaggg cgccgugcac     1380

accgcccugg ccggggcgcu ggaggccgag auggacggcg ccaagggccg ccucuucucc     1440

gggcaccuga agugccggcu gaagauggac aagcuccgcc ugaagggcgu gagcuacucc     1500

cugugcacgg ccgccuucac cuucaccaag guccccgcgg agacccucca cgggaccgug     1560

acgguggagg uccaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1620

guggacaugc agacccugac cccggucggg cggcugauca cggccaaccc ggugaucacc     1680

gagagcaccg agaacuccaa gaugaugcuc gagcuggacc cgcccuucgg cgacagcuac     1740

aucgugaucg gggucggcga caagaagauc acccaccacu ggcaccgcuc cgggagcacg     1800

aucggcaagg ccuucgaggc gaccgugcgg ggcgccaagc gcauggccgu gcugggggac     1860

accgccuggg acuucggcag cgucgggggc guguucaacu cccuggggaa gggcauccac     1920

cagaucuucg gcgccgcguu caagagccuc uucgggggca uguccugguu cagccagauc     1980

cugaucggga cccugcucgu guggcugggc cugaacacga agaacggguc caucagccuc     2040

accugccugg cccugggcgg cgucaugauc uuccucucca ccgccgugag cgccgacgug     2100

gggugcuccg ucgacuucag caag                                            2124


<210>  162
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS mRNA GC

<400>  162
ucccagaagg ugaucuaccu cgucaugauc cugcugaucg ccccggccua cagcauccgc       60

ugcaucggcg uguccaaccg ggacuucgug gaggggauga gcggcgggac gugggucgac      120

guggugcucg agcacggcgg cugcgucacc gugauggcgc aggacaagcc caccguggac      180

aucgagcugg ucaccacgac cguguccaac auggccgagg uccgcagcua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cccaggggga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcacgc ucguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caagggcucc cuggucacgu gcgcgaaguu cacgugcagc      420

aagaagauga ccgggaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc      480

gugcacggcu cccagcacag cgggaugauc ggcuacgaga cggacgagga ccgggccaag      540

guggagguca ccccgaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccuc      600

gggcuggacu gcgagccccg gaccggccug gacuucuccg accucuacua ccugacgaug      660

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu cccguggcac      720

gcgggggccg acaccggcac cccgcacugg aacaacaagg aggcccuggu ggaguucaag      780

gacgcccacg ccaagcgcca gaccgucgug gugcugggca gccaggaggg ggcgguccac      840

acggcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggccg gcuguucucc      900

gggcaccuga agugccgccu caagauggac aagcugcggc ugaagggcgu gagcuacucc      960

cucugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcacgguc     1020

accguggagg ugcaguacgc cgggaccgac ggccccugca agaucccggu ccagauggcg     1080

guggacaugc agacccugac gcccgugggg cgccucauca ccgccaaccc cgucaucacc     1140

gagagcaccg agaacuccaa gaugaugcug gagcuggacc cgccguucgg cgacagcuac     1200

aucgugaucg gggugggcga caagaagauc acgcaccacu ggcaccggag cggcuccacc     1260

aucgggaagg ccuucgaggc caccguccgc ggcgcgaagc ggauggccgu gcucggggac     1320

accgccuggg acuucggcag c                                               1341


<210>  163
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS mRNA GC

<400>  163
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc gacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcucuucgg caaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc ggcuacgaga ccgacgagga ccgggccaag      540

guggagguga cccccaacuc cccgcgcgcc gaggccacgc ugggcggguu cggcagccug      600

gggcucgacu gcgagccccg gaccggccug gacuucuccg accuguacua ccucaccaug      660

aacaacaagc acuggcuggu ccacaaggag ugguuccacg acaucccccu gcccuggcac      720

gcgggggccg acaccggcac gccgcacugg aacaacaagg aggcccucgu ggaguucaag      780

gacgcccacg ccaagcgcca gaccgugguc gugcugggga gccaggaggg cgcggugcac      840

accgcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggccg gcuguucagc      900

gggcaccuga agugccgccu caagauggac aagcugcggc ugaagggcgu cuccuacagc      960

cucugcaccg ccgccuucac guucaccaag gugcccgccg agacccugca cgggaccguc     1020

acgguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcg     1080

guggacaugc agacccugac cccggugggg cgccucauca cggccaaccc ggucaucacc     1140

gaguccaccg agaacagcaa gaugaugcug gagcuggacc cgcccuucgg cgacuccuac     1200

aucgugaucg gggugggcga caagaagauc acccaccacu ggcaccggag cggguccacg     1260

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc ggauggcggu gcucggggac     1320

accgccuggg acuucggcag c                                               1341


<210>  164
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS mRNA optimized

<400>  164
cggaucauca acgcccggaa agagaagaag cgcagaggcg ccgauaccag cgugggcauu       60

gugggccugc ugcugacaac agccauggcc gccgaaguga ccagaagagg cagcgccuac      120

uacauguacc uggaccggaa cgaugccggc gaggccauca gcuuuccaac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gaacccgacg auguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugucaccaca agaagggcga agccagacgg      360

uccagacggg ccgugacacu gccuagccac agcaccagaa agcugcagac ccggucccag      420

accuggcugg aaagcagaga guacaccaag caccugaucc ggguggaaaa cuggaucuuc      480

cggaaccccg gcuuugcccu ggccgcugcu gcuauugcuu ggcugcuggg cagcagcacc      540

ucccagaaag ugaucuaccu cgugaugauc cugcugaucg ccccugccua cagcauccgg      600

uguaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      660

aucgugcugg aacacggcgg cugcgugaca gugauggccc aggauaagcc caccguggau      720

aucgagcucg ugaccaccac cguguccaau auggccgaag ugcggagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccua cacagggcga ggccuaccug      840

gacaagcagu ccgacaccca guacgugugc aagcggaccc ugguggauag aggcuggggc      900

aauggcugcg gccuguuugg caagggcagc cucgugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc     1020

gugcacggca gccagcacuc cggcaugauc gugaacgaca ccggccacga gacagacgag     1080

aaccgggcca agguggaaau cacccccaac agcccuagag ccgaggccac acugggcggc     1140

uuuggaucuc ugggccugga cugcgagccu agaaccggcc uggauuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaaag agugguucca cgacaucccc     1260

cugcccuggc augccggcgc ugauacaggc acaccccacu ggaacaacaa agaggcucug     1320

guggaguuca aggacgccca cgccaagagg cagaccgugg uggugcuggg aucucaggaa     1380

ggcgccgugc auacagcucu ggcuggcgcc cuggaagccg aaauggaugg cgcuaagggc     1440

agacugucca gcggccaccu gaagugccgg cugaagaugg acaagcugcg gcugaagggc     1500

guguccuaca gccuguguac cgccgccuuc accuucacca agauccccgc cgagacacug     1560

cacggcaccg ugacugugga agugcaguac gccggcaccg acggcccuug uaaagugccu     1620

gcucagaugg cuguggacau gcagacccug accccugugg gcaggcugau caccgccaac     1680

ccugugauca ccgagagcac cgagaacagc aagaugaugc uggaacugga cccacccuuc     1740

ggcgacagcu acaucgugau cggcgugggg gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuuugag gcuacagugc ggggagccaa gagaauggcc     1860

gugcugggag auaccgccug ggacuuuggc ucugugggcg gagcccugaa cucucugggc     1920

aagggaaucc accagaucuu cggagccgcc uuuaagagcc uguucggcgg cauguccugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucuccc ugaugugccu ggcucuggga ggcgugcuga ucuuccugag cacagccgug     2100

ucugccgacg ugggcugcag cguggacuuc agcaaa                               2136


<210>  165
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723)  CDS mRNA 
       optimized

<400>  165
ucccagaaag ugaucuaccu cgugaugauc cugcugaucg ccccugccua cagcauccgg       60

uguaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      120

aucgugcugg aacacggcgg cugcgugaca gugauggccc aggauaagcc caccguggau      180

aucgagcucg ugaccaccac cguguccaau auggccgaag ugcggagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccua cacagggcga ggccuaccug      300

gacaagcagu ccgacaccca guacgugugc aagcggaccc ugguggauag aggcuggggc      360

aauggcugcg gccuguuugg caagggcagc cucgugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc      480

gugcacggca gccagcacuc cggcaugauc gugaacgaca ccggccacga gacagacgag      540

aaccgggcca agguggaaau cacccccaac agcccuagag ccgaggccac acugggcggc      600

uuuggaucuc ugggccugga cugcgagccu agaaccggcc uggauuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaaag agugguucca cgacaucccc      720

cugcccuggc augccggcgc ugauacaggc acaccccacu ggaacaacaa agaggcucug      780

guggaguuca aggacgccca cgccaagagg cagaccgugg uggugcuggg aucucaggaa      840

ggcgccgugc auacagcucu ggcuggcgcc cuggaagccg aaauggaugg cgcuaagggc      900

agacugucca gcggccaccu gaagugccgg cugaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccuguguac cgccgccuuc accuucacca agauccccgc cgagacacug     1020

cacggcaccg ugacugugga agugcaguac gccggcaccg acggcccuug uaaagugccu     1080

gcucagaugg cuguggacau gcagacccug accccugugg gcaggcugau caccgccaac     1140

ccugugauca ccgagagcac cgagaacagc aagaugaugc uggaacugga cccacccuuc     1200

ggcgacagcu acaucgugau cggcgugggg gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuuugag gcuacagugc ggggagccaa gagaauggcc     1320

gugcugggag auaccgccug ggacuuuggc ucu                                  1353


<210>  166
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS mRNA optimized

<400>  166
cggaucauca acgcccggaa agagaagaag cgcagaggcg ccgauaccag cgugggcauu       60

gugggccugc ugcugacaac agccauggcc gccgaaguga ccagaagagg cagcgccuac      120

uacauguacc uggaccggaa cgaugccggc gaggccauca gcuuuccaac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ccugugacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gaacccgacg auguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugucaccaca agaagggcga agccagacgg      360

uccagacggg ccgugacacu gccuagccac agcaccagaa agcugcagac ccggucccag      420

accuggcugg aaagcagaga guacaccaag caccugaucc ggguggaaaa cuggaucuuc      480

cggaaccccg gcuuugcccu ggccgcugcu gcuauugcuu ggcugcuggg cagcagcacc      540

ucccagaaag ugaucuaccu cgugaugauc cugcugaucg ccccugccua cagcauccgg      600

uguaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      660

guggugcugg aacauggcgg cugcgugaca gugauggcuc aggacaagcc caccguggac      720

aucgagcucg ugaccaccac cguguccaau auggccgaag ugcggagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccua cacagggcga ggccuaccug      840

gacaagcagu ccgacaccca guacgugugc aagcggaccc ugguggauag aggcuggggc      900

aauggcugcg gccuguuugg caagggcagc cucgugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc     1020

gugcacggca gccagcacuc cggcaugauc gugaacgaca ccggccacga gacagacgag     1080

aaccgggcca agguggaaau cacccccaac agcccuagag ccgaggccac acugggcggc     1140

uuuggaucuc ugggccugga cugcgagccu agaaccggcc uggauuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaaag agugguucca cgacaucccc     1260

cugcccuggc augccggcgc ugauacaggc acaccccacu ggaacaacaa agaggcucug     1320

guggaguuca aggacgccca cgccaagagg cagaccgugg uggugcuggg aucucaggaa     1380

ggcgccgugc auacagcucu ggcuggcgcc cuggaagccg aaauggaugg cgcuaagggc     1440

agacugucca gcggccaccu gaagugccgg cugaagaugg acaagcugcg gcugaagggc     1500

guguccuaca gccuguguac cgccgccuuc accuucacca agauccccgc cgagacacug     1560

cacggcaccg ugacugugga agugcaguac gccggcaccg acggcccuug uaaagugccu     1620

gcucagaugg ccguggauau gcagacccug accccugugg gcaggcugau caccgccaac     1680

ccugugauca ccgagagcac cgagaacagc aagaugaugc uggaacugga cccacccuuc     1740

ggcgacagcu acaucgugau cggcgugggg gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuuugag gcuacagugc ggggagccaa gagaauggcc     1860

gugcugggag auaccgccug ggacuuuggc ucugugggcg gagcccugaa cucucugggc     1920

aagggaaucc accagaucuu cggagccgcc uuuaagagcc uguucggcgg cauguccugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacgc caagaacggc     2040

agcaucuccc ugaugugccu ggcucuggga ggcgugcuga ucuuccugag cacagccgug     2100

ucugccgacg ugggcugcag cguggacuuc agcaaa                               2136


<210>  167
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723)  CDS mRNA optimized

<400>  167
ucccagaaag ugaucuaccu cgugaugauc cugcugaucg ccccugccua cagcauccgg       60

uguaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      120

guggugcugg aacauggcgg cugcgugaca gugauggcuc aggacaagcc caccguggac      180

aucgagcucg ugaccaccac cguguccaau auggccgaag ugcggagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccua cacagggcga ggccuaccug      300

gacaagcagu ccgacaccca guacgugugc aagcggaccc ugguggauag aggcuggggc      360

aauggcugcg gccuguuugg caagggcagc cucgugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc      480

gugcacggca gccagcacuc cggcaugauc gugaacgaca ccggccacga gacagacgag      540

aaccgggcca agguggaaau cacccccaac agcccuagag ccgaggccac acugggcggc      600

uuuggaucuc ugggccugga cugcgagccu agaaccggcc uggauuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaaag agugguucca cgacaucccc      720

cugcccuggc augccggcgc ugauacaggc acaccccacu ggaacaacaa agaggcucug      780

guggaguuca aggacgccca cgccaagagg cagaccgugg uggugcuggg aucucaggaa      840

ggcgccgugc auacagcucu ggcuggcgcc cuggaagccg aaauggaugg cgcuaagggc      900

agacugucca gcggccaccu gaagugccgg cugaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccuguguac cgccgccuuc accuucacca agauccccgc cgagacacug     1020

cacggcaccg ugacugugga agugcaguac gccggcaccg acggcccuug uaaagugccu     1080

gcucagaugg ccguggauau gcagacccug accccugugg gcaggcugau caccgccaac     1140

ccugugauca ccgagagcac cgagaacagc aagaugaugc uggaacugga cccacccuuc     1200

ggcgacagcu acaucgugau cggcgugggg gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuuugag gcuacagugc ggggagccaa gagaauggcc     1320

gugcugggag auaccgccug ggacuuuggc ucu                                  1353


<210>  168
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS mRNA optimized

<400>  168
cggaucauca acgcccggaa agagcggaag cggagaggcg ccgauaccag caucggcauc       60

aucggccugc ugcugaccac agccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuuugccac aacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gaacccgacg auguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugucaccaca agaagggcga ggccagacgg      360

ucuagaaggg ccgugacacu gccuagccac agcacccgga agcugcagac cagaagccag      420

accuggcugg aaagcagaga guacaccaag caccugauca agguggaaaa cuggaucuuc      480

cggaaccccg gcuucgcccu gguggccgug gcuauugcuu ggcugcuggg cagcuccacc      540

ucccagaaag ugaucuaccu cgugaugauc cugcugaucg ccccugccua cagcauccgg      600

uguaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      660

guggugcugg aacauggcgg cugcgugaca gugauggccc aggacaagcc caccguggac      720

aucgagcucg ugaccaccac cguguccaau auggccgaag ugcggagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccua cacaggggga ggccuaccug      840

gauaagcagu ccgacaccca guacgugugc aagcggaccc ugguggauag aggcuggggc      900

aauggcugcg gccuguuugg caagggcagc cucgugaccu gcgccaaguu caccuguagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc     1020

gugcacggca gccagcacag cggaaugauc ggcuacgaga cagacgagga ccgggccaaa     1080

guggaaguga cccccaacag cccuagagcc gaggccacac ugggcggcuu uggaucucug     1140

ggccuggacu gcgagccuag aaccggccug gauuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaagag ugguuccacg acaucccccu gcccuggcau     1260

gccggcgcug auacaggcac accccacugg aacaacaaag aggcucuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggcu cucaggaagg cgccgugcau     1380

acagcucugg cuggcgcccu ggaagccgaa auggauggcg ccaagggcag acuguucagc     1440

ggccaccuga agugccggcu gaagauggac aagcugcggc ugaagggcgu guccuacagc     1500

cuguguaccg ccgccuucac cuucaccaag gugcccgccg aaacccugca cggcacagug     1560

acuguggaag ugcaguacgc cggcaccgac ggcccuugca agaucccugu gcagauggcc     1620

guggauaugc agacccugac cccugugggc aggcugauca ccgccaaccc ugugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gaacuggacc cacccuucgg cgacagcuac     1740

aucgugaucg gcgugggaga caagaagauc acccaccacu ggcaccgcag cggcagcaca     1800

aucggaaagg ccuucgaagc cacagugcgg ggagccaaga gaauggccgu gcugggagau     1860

accgccuggg acuuuggcuc ugugggcggc guguucaacu cccugggcaa gggaauccac     1920

cagaucuucg gagccgccuu uaagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcucgu guggcugggc cugaacacca agaacggcag caucucccug     2040

accugccugg cucugggcgg agugaugauc uuccugagca ccgccguguc cgccgaugug     2100

ggcuguagcg uggacuucag caaa                                            2124


<210>  169
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719)  CDS mRNA optimized

<400>  169
ucccagaaag ugaucuaccu cgugaugauc cugcugaucg ccccugccua cagcauccgg       60

uguaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      120

guggugcugg aacauggcgg cugcgugaca gugauggccc aggacaagcc caccguggac      180

aucgagcucg ugaccaccac cguguccaau auggccgaag ugcggagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccua cacaggggga ggccuaccug      300

gauaagcagu ccgacaccca guacgugugc aagcggaccc ugguggauag aggcuggggc      360

aauggcugcg gccuguuugg caagggcagc cucgugaccu gcgccaaguu caccuguagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc      480

gugcacggca gccagcacag cggaaugauc ggcuacgaga cagacgagga ccgggccaaa      540

guggaaguga cccccaacag cccuagagcc gaggccacac ugggcggcuu uggaucucug      600

ggccuggacu gcgagccuag aaccggccug gauuucagcg accuguacua ccugaccaug      660

aacaacaagc acuggcuggu gcacaaagag ugguuccacg acaucccccu gcccuggcau      720

gccggcgcug auacaggcac accccacugg aacaacaaag aggcucuggu ggaguucaag      780

gacgcccacg ccaagagaca gaccguggug gugcugggcu cucaggaagg cgccgugcau      840

acagcucugg cuggcgcccu ggaagccgaa auggauggcg ccaagggcag acuguucagc      900

ggccaccuga agugccggcu gaagauggac aagcugcggc ugaagggcgu guccuacagc      960

cuguguaccg ccgccuucac cuucaccaag gugcccgccg aaacccugca cggcacagug     1020

acuguggaag ugcaguacgc cggcaccgac ggcccuugca agaucccugu gcagauggcc     1080

guggauaugc agacccugac cccugugggc aggcugauca ccgccaaccc ugugaucacc     1140

gagagcaccg agaacagcaa gaugaugcug gaacuggacc cacccuucgg cgacagcuac     1200

aucgugaucg gcgugggaga caagaagauc acccaccacu ggcaccgcag cggcagcaca     1260

aucggaaagg ccuucgaagc cacagugcgg ggagccaaga gaauggccgu gcugggagau     1320

accgccuggg acuuuggcuc u                                               1341


<210>  170
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS mRNA optimized

<400>  170
cggaucauca acgcccggaa agagaagaag cgcagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugacaac cgccauggcc gccgaaguga ccagacgggg cagcgccuac      120

uacauguacc uggaccggaa cgacgccggc gaggccauca gcuuucccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gaacccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugucaccaca agaagggcga ggccagacgg      360

uccagacggg ccgugacacu gcccagccac agcacccgga agcugcagac cagaagccag      420

accuggcugg aaagcagaga guacaccaag caccugaucc ggguggaaaa cuggaucuuc      480

cggaaccccg gcuucgcccu ggccgcugcc gcuauugcuu ggcugcuggg cagcagcacc      540

agccagaaag ugaucuaccu ggugaugauc cugcugaucg ccccugccua cagcauccgg      600

ugcaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      660

aucgugcugg aacacggcgg cugcgugacc gugauggccc aggacaagcc caccguggau      720

aucgagcugg ugacaaccac cguguccaac auggccgaag ugcggagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccaggggga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcggaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguuugg caagggcagc cuggugacau gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gacagacgag     1080

aaccgggcca agguggaaau cacccccaac agccccagag ccgaggccac acugggcggc     1140

uuuggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaaag agugguucca cgacaucccc     1260

cugcccuggc acgcuggcgc cgauacaggc accccucacu ggaacaacaa agaggcccug     1320

gucgaguuca aggacgccca cgccaagcgg cagaccgugg uggugcuggg aucucaggaa     1380

ggcgccgucc acacagcccu ggcuggcgcc cuggaagccg agauggaugg cgccaagggc     1440

aggcugagca gcggccaccu gaagugccgg cugaagaugg acaagcugcg gcugaagggc     1500

guguccuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacacug     1560

cacggcaccg ugaccgugga agugcaguac gccggcaccg acggcccuug uaaggugcca     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gccggcugau caccgccaac     1680

ccugugauca ccgagagcac cgagaacagc aagaugaugc uggaacugga cccacccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccacagugc ggggagccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuuugga ucugugggcg gagcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggagccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcauuagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg ugggcugcag cguggacuuc uccaaa                               2136


<210>  171
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723)  CDS mRNA 
       optimized

<400>  171
agccagaaag ugaucuaccu ggugaugauc cugcugaucg ccccugccua cagcauccgg       60

ugcaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      120

aucgugcugg aacacggcgg cugcgugacc gugauggccc aggacaagcc caccguggau      180

aucgagcugg ugacaaccac cguguccaac auggccgaag ugcggagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccaggggga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcggaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguuugg caagggcagc cuggugacau gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gacagacgag      540

aaccgggcca agguggaaau cacccccaac agccccagag ccgaggccac acugggcggc      600

uuuggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaaag agugguucca cgacaucccc      720

cugcccuggc acgcuggcgc cgauacaggc accccucacu ggaacaacaa agaggcccug      780

gucgaguuca aggacgccca cgccaagcgg cagaccgugg uggugcuggg aucucaggaa      840

ggcgccgucc acacagcccu ggcuggcgcc cuggaagccg agauggaugg cgccaagggc      900

aggcugagca gcggccaccu gaagugccgg cugaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacacug     1020

cacggcaccg ugaccgugga agugcaguac gccggcaccg acggcccuug uaaggugcca     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gccggcugau caccgccaac     1140

ccugugauca ccgagagcac cgagaacagc aagaugaugc uggaacugga cccacccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccacagugc ggggagccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuuugga ucu                                  1353


<210>  172
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS mRNA optimized

<400>  172
cggaucauca acgcccggaa agagaagaag cgcagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugacaac cgccauggcc gccgaaguga ccagacgggg cagcgccuac      120

uacauguacc uggaccggaa cgacgccggc gaggccauca gcuuucccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ccugugacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gaacccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugucaccaca agaagggcga ggccagacgg      360

uccagacggg ccgugacacu gcccagccac agcacccgga agcugcagac cagaagccag      420

accuggcugg aaagcagaga guacaccaag caccugaucc ggguggaaaa cuggaucuuc      480

cggaaccccg gcuucgcccu ggccgcugcc gcuauugcuu ggcugcuggg cagcagcacc      540

agccagaaag ugaucuaccu ggugaugauc cugcugaucg ccccugccua cagcauccgg      600

ugcaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      660

guggugcugg aacacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugacaaccac cguguccaac auggccgaag ugcggagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccaggggga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcggaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguuugg caagggcagc cuggugacau gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gacagacgag     1080

aaccgggcca agguggaaau cacccccaac agccccagag ccgaggccac acugggcggc     1140

uuuggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaaag agugguucca cgacaucccc     1260

cugcccuggc acgcuggcgc cgauacaggc accccucacu ggaacaacaa agaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgugg uggugcuggg aucucaggaa     1380

ggcgccgugc acacagcccu ggcuggcgcc cuggaagccg agauggaugg cgccaagggc     1440

aggcugagca gcggccaccu gaagugccgg cugaagaugg acaagcugcg gcugaagggc     1500

guguccuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacacug     1560

cacggcaccg ugaccgugga agugcaguac gccggcaccg acggcccuug caaggugcca     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gccggcugau caccgccaac     1680

ccugugauca ccgagagcac cgagaacagc aagaugaugc uggaacugga cccacccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccacagugc ggggagccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuuugga ucugugggcg gagcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggagccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacgc caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg ugggcugcag cguggacuuc uccaaa                               2136


<210>  173
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723)  CDS mRNA optimized

<400>  173
agccagaaag ugaucuaccu ggugaugauc cugcugaucg ccccugccua cagcauccgg       60

ugcaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      120

guggugcugg aacacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugacaaccac cguguccaac auggccgaag ugcggagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccaggggga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcggaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguuugg caagggcagc cuggugacau gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gacagacgag      540

aaccgggcca agguggaaau cacccccaac agccccagag ccgaggccac acugggcggc      600

uuuggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaaag agugguucca cgacaucccc      720

cugcccuggc acgcuggcgc cgauacaggc accccucacu ggaacaacaa agaggcccug      780

guggaguuca aggacgccca cgccaagcgg cagaccgugg uggugcuggg aucucaggaa      840

ggcgccgugc acacagcccu ggcuggcgcc cuggaagccg agauggaugg cgccaagggc      900

aggcugagca gcggccaccu gaagugccgg cugaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacacug     1020

cacggcaccg ugaccgugga agugcaguac gccggcaccg acggcccuug caaggugcca     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gccggcugau caccgccaac     1140

ccugugauca ccgagagcac cgagaacagc aagaugaugc uggaacugga cccacccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccacagugc ggggagccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuuugga ucu                                  1353


<210>  174
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS mRNA optimized

<400>  174
cggaucauca acgcccggaa agagcggaag cggagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagacgggg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gaacccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugucaccaca agaagggcga ggccagacgg      360

uccagacggg ccgugacacu gcccagccac agcacccgga agcugcagac cagaagccag      420

accuggcugg aaagcagaga guacaccaag caccugauca agguggaaaa cuggaucuuc      480

cggaaccccg gcuucgcccu gguggccgug gcuauugccu ggcugcuggg cagcagcacc      540

agccagaaag ugaucuaccu ggugaugauc cugcugaucg ccccugccua cagcauccgg      600

ugcaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      660

guggugcugg aacacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugacaaccac cguguccaac auggccgaag ugcggagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccaggggga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcggaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguuugg caagggcagc cuggugacau gcgccaaguu caccuguagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc ggcuacgaga cagacgagga ccgggccaag     1080

guggaaguga cccccaacag cccuagagcc gaggccacac ugggcggcuu uggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaagag ugguuccacg acaucccccu gcccuggcac     1260

gcuggcgccg auacaggcac cccucacugg aacaacaaag aggcccuggu ggaguucaag     1320

gacgcccacg ccaagcggca gaccguggug gugcugggau cucaggaagg cgccgugcac     1380

acagcccugg cuggcgcccu ggaagccgag auggauggcg ccaagggccg gcuguucagc     1440

ggccaccuga agugccggcu gaagauggac aagcugcggc ugaagggcgu guccuacagc     1500

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacacugca cggcaccgug     1560

accguggaag ugcaguacgc cggcaccgac ggccccugca agaucccugu gcagauggcc     1620

guggacaugc agacccugac ccccgugggc cggcugauca ccgccaaccc ugugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gaacuggacc cacccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc cacagugcgg ggagccaaga gaauggccgu gcugggcgac     1860

accgccuggg auuuuggcuc cgugggcggc guguucaaca gccugggcaa gggcauccac     1920

cagaucuucg gagccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcuggu cuggcugggc cugaacacca agaacggcag caucagccug     2040

accugccugg cccugggcgg agugaugauc uuccugagca ccgccguguc ugccgacgug     2100

ggcugcagcg uggacuucuc caaa                                            2124


<210>  175
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719)  CDS mRNA optimized

<400>  175
agccagaaag ugaucuaccu ggugaugauc cugcugaucg ccccugccua cagcauccgg       60

ugcaucggcg uguccaaccg ggacuucgug gaaggcauga gcggcggcac auggguggac      120

guggugcugg aacacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugacaaccac cguguccaac auggccgaag ugcggagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccaggggga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcggaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguuugg caagggcagc cuggugacau gcgccaaguu caccuguagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aauaccggau caugcugagc      480

gugcacggca gccagcacag cggcaugauc ggcuacgaga cagacgagga ccgggccaag      540

guggaaguga cccccaacag cccuagagcc gaggccacac ugggcggcuu uggcagccug      600

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug      660

aacaacaagc acuggcuggu gcacaaagag ugguuccacg acaucccccu gcccuggcac      720

gcuggcgccg auacaggcac cccucacugg aacaacaaag aggcccuggu ggaguucaag      780

gacgcccacg ccaagcggca gaccguggug gugcugggau cucaggaagg cgccgugcac      840

acagcccugg cuggcgcccu ggaagccgag auggauggcg ccaagggccg gcuguucagc      900

ggccaccuga agugccggcu gaagauggac aagcugcggc ugaagggcgu guccuacagc      960

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacacugca cggcaccgug     1020

accguggaag ugcaguacgc cggcaccgac ggccccugca agaucccugu gcagauggcc     1080

guggacaugc agacccugac ccccgugggc cggcugauca ccgccaaccc ugugaucacc     1140

gagagcaccg agaacagcaa gaugaugcug gaacuggacc cacccuucgg cgacagcuac     1200

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1260

aucggcaagg ccuucgaggc cacagugcgg ggagccaaga gaauggccgu gcugggcgac     1320

accgccuggg auuuuggcuc c                                               1341


<210>  176
<211>  10269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein CDS mRNA optimized

<400>  176
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gcggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc      360

auggccgccg aggugacccg ccgcggcagc gccuacuaca uguaccugga ccgcaacgac      420

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      480

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugauccgcgu ggagaacugg aucuuccgca accccggcuu cgcccuggcc      780

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucguga acgacaccgg ccacgagacc gacgagaacc gcgccaaggu ggagaucacc     1380

cccaacagcc cccgcgccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1440

gagccccgca ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1560

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1620

aagcgccaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1680

ggcgcccugg aggccgagau ggacggcgcc aagggccgcc ugagcagcgg ccaccugaag     1740

ugccgccuga agauggacaa gcugcgccug aagggcguga gcuacagccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1860

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1920

acccugaccc ccgugggccg ccugaucacc gccaaccccg ugaucaccga gagcaccgag     1980

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     2040

gugggcgaga agaagaucac ccaccacugg caccgcagcg gcagcaccau cggcaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc ugggcgacac cgccugggac     2160

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     2220

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     2280

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2340

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     2400

gacuucagca agaaggagac ccgcugcggc accggcgugu ucguguacaa cgacguggag     2460

gccuggcgcg accgcuacaa guaccacccc gacagccccc gccgccuggc cgccgccgug     2520

aagcaggccu gggaggacgg caucugcggc aucagcagcg ugagccgcau ggagaacauc     2580

auguggcgca gcguggaggg cgagcugaac gccauccugg aggagaacgg cgugcagcug     2640

accguggugg ugggcagcgu gaagaacccc auguggcgcg gcccccagcg ccugcccgug     2700

cccgugaacg agcugcccca cggcuggaag gccuggggca agagccacuu cgugcgcgcc     2760

gccaagacca acaacagcuu cgugguggac ggcgacaccc ugaaggagug cccccugaag     2820

caccgcgccu ggaacagcuu ccugguggag gaccacggcu ucggcguguu ccacaccagc     2880

guguggcuga aggugcgcga ggacuacagc cuggagugcg accccgccgu gaucggcacc     2940

gccgugaagg gcaaggaggc cgugcacagc gaccugggcu acuggaucga gagcgagaag     3000

aacgacaccu ggcgccugaa gcgcgcccac cugaucgaga ugaagaccug cgaguggccc     3060

aagagccaca cccuguggac cgacggcauc gaggagagcg accugaucau ccccaagagc     3120

cuggccggcc cccugagcca ccacaacacc cgcgagggcu accgcaccca gaugaagggc     3180

cccuggcaca gcgaggagcu ggagauccgc uucgaggagu gccccggcac caaggugcac     3240

guggaggaga ccugcggcac ccgcggcccc agccugcgca gcaccaccgc cagcggccgc     3300

gugaucgagg aguggugcug ccgcgagugc accaugcccc cccugagcuu ccgcgccaag     3360

gacggcugcu gguacggcau ggagauccgc ccccgcaagg agcccgagag caaccuggug     3420

cgcagcaugg ugaccgccgg cagcaccgac cacauggacc acuucagccu gggcgugcug     3480

gugauccugc ugauggugca ggagggccug aagaagcgca ugaccaccaa gaucaucauc     3540

agcaccagca uggccgugcu gguggccaug auccugggcg gcuucagcau gagcgaccug     3600

gccaagcugg ccauccugau gggcgccacc uucgccgaga ugaacaccgg cggcgacgug     3660

gcccaccugg cccugaucgc cgccuucaag gugcgccccg cccugcuggu gagcuucauc     3720

uuccgcgcca acuggacccc ccgcgagagc augcugcugg cccuggccag cugccugcug     3780

cagaccgcca ucagcgcccu ggagggcgac cugauggugc ugaucaacgg cuucgcccug     3840

gccuggcugg ccauccgcgc caugguggug ccccgcaccg acaacaucac ccuggccauc     3900

cuggccgccc ugaccccccu ggcccgcggc acccugcugg uggccuggcg cgccggccug     3960

gccaccugcg gcggcuucau gcugcugagc cugaagggca agggcagcgu gaagaagaac     4020

cugcccuucg ugauggcccu gggccugacc gccgugcgcc ugguggaccc caucaacgug     4080

gugggccugc ugcugcugac ccgcagcggc aagcgcagcu ggccccccag cgaggugcug     4140

accgccgugg gccugaucug cgcccuggcc ggcggcuucg ccaaggccga caucgagaug     4200

gccggcccca uggccgccgu gggccugcug aucgugagcu acguggugag cggcaagagc     4260

guggacaugu acaucgagcg cgccggcgac aucaccuggg agaaggacgc cgaggugacc     4320

ggcaacagcc cccgccugga cguggcccug gacgagagcg gcgacuucag ccugguggag     4380

gacgacggcc cccccaugcg cgagaucauc cugaaggugg ugcugaugac caucugcggc     4440

augaacccca ucgccauccc cuucgccgcc ggcgccuggu acguguacgu gaagaccggc     4500

aagcgcagcg gcgcccugug ggacgugccc gcccccaagg aggugaagaa gggcgagacc     4560

accgacggcg uguaccgcgu gaugacccgc cgccugcugg gcagcaccca ggugggcgug     4620

ggcgugaugc aggagggcgu guuccacacc auguggcacg ugaccaaggg cagcgcccug     4680

cgcagcggcg agggccgccu ggaccccuac uggggcgacg ugaagcagga ccuggugagc     4740

uacugcggcc ccuggaagcu ggacgccgcc ugggacggcc acagcgaggu gcagcugcug     4800

gccgugcccc ccggcgagcg cgcccgcaac auccagaccc ugcccggcau cuucaagacc     4860

aaggacggcg acaucggcgc cguggcccug gacuaccccg ccggcaccag cggcagcccc     4920

auccuggaca agugcggccg cgugaucggc cuguacggca acggcguggu gaucaagaac     4980

ggcagcuacg ugagcgccau cacccagggc cgccgcgagg aggagacccc cguggagugc     5040

uucgagccca gcaugcugaa gaagaagcag cugaccgugc uggaccugca ccccggcgcc     5100

ggcaagaccc gccgcgugcu gcccgagauc gugcgcgagg ccaucaagac ccgccugcgc     5160

accgugaucc uggcccccac ccgcguggug gccgccgaga uggaggaggc ccugcgcggc     5220

cugcccgugc gcuacaugac caccgccgug aacgugaccc acagcggcac cgagaucgug     5280

gaccugaugu gccacgccac cuucaccagc cgccugcugc agcccauccg cgugcccaac     5340

uacaaccugu acaucaugga cgaggcccac uucaccgacc ccagcagcau cgccgcccgc     5400

ggcuacauca gcacccgcgu ggagaugggc gaggccgccg ccaucuucau gaccgccacc     5460

ccccccggca cccgcgacgc cuuccccgac agcaacagcc ccaucaugga caccgaggug     5520

gaggugcccg agcgcgccug gagcagcggc uucgacuggg ugaccgacua cagcggcaag     5580

accguguggu ucgugcccag cgugcgcaac ggcaacgaga ucgccgccug ccugaccaag     5640

gccggcaagc gcgugaucca gcugagccgc aagaccuucg agaccgaguu ccagaagacc     5700

aagcaccagg agugggacuu cguggugacc accgacauca gcgagauggg cgccaacuuc     5760

aaggccgacc gcgugaucga cagccgccgc ugccugaagc ccgugauccu ggacggcgag     5820

cgcgugaucc uggccggccc caugcccgug acccacgcca gcgccgccca gcgccgcggc     5880

cgcaucggcc gcaaccccaa caagcccggc gacgaguacc uguacggcgg cggcugcgcc     5940

gagaccgacg aggaccacgc ccacuggcug gaggcccgca ugcugcugga caacaucuac     6000

cugcaggacg gccugaucgc cagccuguac cgccccgagg ccgacaaggu ggccgccauc     6060

gagggcgagu ucaagcugcg caccgagcag cgcaagaccu ucguggagcu gaugaagcgc     6120

ggcgaccugc ccguguggcu ggccuaccag guggccagcg ccggcaucac cuacaccgac     6180

cgccgcuggu gcuucgacgg caccaccaac aacaccauca uggaggacag cgugcccgcc     6240

gaggugugga cccgccacgg cgagaagcgc gugcugaagc cccgcuggau ggacgcccgc     6300

gugugcagcg accacgccgc ccugaagagc uucaaggagu ucgccgccgg caagcgcggc     6360

gccgccuucg gcgugaugga ggcccugggc acccugcccg gccacaugac cgagcgcuuc     6420

caggaggcca ucgacaaccu ggccgugcug augcgcgccg agaccggcag ccgccccuac     6480

aaggccgccg ccgcccagcu gcccgagacc cuggagacca ucaugcugcu gggccugcug     6540

ggcaccguga gccugggcau cuucuucgug cugaugcgca acaagggcau cggcaagaug     6600

ggcuucggca uggugacccu gggcgccagc gccuggcuga uguggcugag cgagaucgag     6660

cccgcccgca ucgccugcgu gcugaucgug guguuccugc ugcugguggu gcugaucccc     6720

gagcccgaga agcagcgcag cccccaggac aaccagaugg ccaucaucau caugguggcc     6780

gugggccugc ugggccugau caccgccaac gagcugggcu ggcuggagcg caccaagagc     6840

gaccugagcc accugauggg ccgccgcgag gagggcgcca ccaugggcuu cagcauggac     6900

aucgaccugc gccccgccag cgccugggcc aucuacgccg cccugaccac cuucaucacc     6960

cccgccgugc agcacgccgu gaccaccagc uacaacaacu acagccugau ggccauggcc     7020

acccaggccg gcgugcuguu cggcaugggc aagggcaugc ccuucuacgc cugggacuuc     7080

ggcgugcccc ugcugaugau cggcugcuac agccagcuga ccccccugac ccugaucgug     7140

gccaucaucc ugcugguggc ccacuacaug uaccugaucc ccggccugca ggccgccgcc     7200

gcccgcgccg cccagaagcg caccgccgcc ggcaucauga agaaccccgu gguggacggc     7260

aucgugguga ccgacaucga caccaugacc aucgaccccc agguggagaa gaagaugggc     7320

caggugcugc ugauggccgu ggccgugagc agcgccaucc ugagccgcac cgccuggggc     7380

uggggcgagg ccggcgcccu gaucaccgcc gccaccagca cccuguggga gggcagcccc     7440

aacaaguacu ggaacagcag caccgccacc agccugugca acaucuuccg cggcagcuac     7500

cuggccggcg ccagccugau cuacaccgug acccgcaacg ccggccuggu gaagcgccgc     7560

ggcggcggca ccggcgagac ccugggcgag aaguggaagg cccgccugaa ccagaugagc     7620

gcccuggagu ucuacagcua caagaagagc ggcaucaccg aggugugccg cgaggaggcc     7680

cgccgcgccc ugaaggacgg cguggccacc ggcggccacg ccgugagccg cggcagcgcc     7740

aagcugcgcu ggcuggugga gcgcggcuac cugcagcccu acggcaaggu gaucgaccug     7800

ggcugcggcc gcggcggcug gagcuacuac gccgccacca uccgcaaggu gcaggaggug     7860

aagggcuaca ccaagggcgg ccccggccac gaggagcccg ugcuggugca gagcuacggc     7920

uggaacaucg ugcgccugaa gagcggcgug gacguguucc acauggccgc cgagcccugc     7980

gacacccugc ugugcgacau cggcgagagc agcagcagcc ccgaggugga ggaggcccgc     8040

acccugcgcg ugcugagcau ggugggcgac uggcuggaga agcgccccgg cgccuucugc     8100

aucaaggugc ugugccccua caccagcacc augauggaga cccuggagcg ccugcagcgc     8160

cgcuacggcg gcggccuggu gcgcgugccc cugagccgca acagcaccca cgagauguac     8220

ugggugagcg gcgccaagag caacaccauc aagagcguga gcaccaccag ccagcugcug     8280

cugggccgca uggacggccc ccgccgcccc gugaaguacg aggaggacgu gaaccugggc     8340

agcggcaccc gcgccguggu gagcugcgcc gaggccccca acaugaagau caucggcaac     8400

cgcaucgagc gcauccgcag cgagcacgcc gagaccuggu ucuucgacga gaaccacccc     8460

uaccgcaccu gggccuacca cggcagcuac gaggccccca cccagggcag cgccagcagc     8520

cugaucaacg gcguggugcg ccugcugagc aagcccuggg acguggugac cggcgugacc     8580

ggcaucgcca ugaccgacac cacccccuac ggccagcagc gcguguucaa ggagaaggug     8640

gacacccgcg ugcccgaccc ccaggagggc acccgccagg ugaugagcau ggugagcagc     8700

uggcugugga aggagcuggg caagcacaag cgcccccgcg ugugcaccaa ggaggaguuc     8760

aucaacaagg ugcgcagcaa cgccgcccug ggcgccaucu ucgaggagga gaaggagugg     8820

aagaccgccg uggaggccgu gaacgacccc cgcuucuggg cccuggugga caaggagcgc     8880

gagcaccacc ugcgcggcga gugccagagc ugcguguaca acaugauggg caagcgcgag     8940

aagaagcagg gcgaguucgg caaggccaag ggcagccgcg ccaucuggua cauguggcug     9000

ggcgcccgcu uccuggaguu cgaggcccug ggcuuccuga acgaggacca cuggaugggc     9060

cgcgagaaca gcggcggcgg cguggagggc cugggccugc agcgccuggg cuacgugcug     9120

gaggagauga gccgcauccc cggcggccgc auguacgccg acgacaccgc cggcugggac     9180

acccgcauca gccgcuucga ccuggagaac gaggcccuga ucaccaacca gauggagaag     9240

ggccaccgcg cccuggcccu ggccaucauc aaguacaccu accagaacaa gguggugaag     9300

gugcugcgcc ccgccgagaa gggcaagacc gugauggaca ucaucagccg ccaggaccag     9360

cgcggcagcg gccagguggu gaccuacgcc cugaacaccu ucaccaaccu gguggugcag     9420

cugauccgca acauggaggc cgaggaggug cuggagaugc aggaccugug gcugcugcgc     9480

cgcagcgaga aggugaccaa cuggcugcag agcaacggcu gggaccgccu gaagcgcaug     9540

gccgugagcg gcgacgacug cguggugaag cccaucgacg accgcuucgc ccacgcccug     9600

cgcuuccuga acgacauggg caaggugcgc aaggacaccc aggaguggaa gcccagcacc     9660

ggcugggaca acugggagga ggugcccuuc ugcagccacc acuucaacaa gcugcaccug     9720

aaggacggcc gcagcaucgu ggugcccugc cgccaccagg acgagcugau cggccgcgcc     9780

cgcgugagcc ccggcgccgg cuggagcauc cgcgagaccg ccugccuggc caagagcuac     9840

gcccagaugu ggcagcugcu guacuuccac cgccgcgacc ugcgccugau ggccaacgcc     9900

aucugcagca gcgugcccgu ggacugggug cccaccggcc gcaccaccug gagcauccac     9960

ggcaagggcg aguggaugac caccgaggac augcuggugg uguggaaccg cguguggauc    10020

gaggagaacg accacaugga ggacaagacc cccgugacca aguggaccga cauccccuac    10080

cugggcaagc gcgaggaccu guggugcggc agccugaucg gccaccgccc ccgcaccacc    10140

ugggccgaga acaucaagaa caccgugaac auggugcgcc gcaucaucgg cgacgaggag    10200

aaguacaugg acuaccugag cacccaggug cgcuaccugg gcgaggaggg cagcaccccc    10260

ggcgugcug                                                            10269


<210>  177
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C CDS mRNA optimized

<400>  177
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gc                                                          312


<210>  178
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-ER anchor CDS mRNA optimized

<400>  178
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gcggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc      360

auggcc                                                                 366


<210>  179
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) pr CDS mRNA optimized

<400>  179
gccgagguga cccgccgcgg cagcgccuac uacauguacc uggaccgcaa cgacgccggc       60

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      120

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgc                             279


<210>  180
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) M CDS mRNA optimized

<400>  180
gccgugaccc ugcccagcca cagcacccgc aagcugcaga cccgcagcca gaccuggcug       60

gagagccgcg aguacaccaa gcaccugauc cgcguggaga acuggaucuu ccgcaacccc      120

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      180

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagc                      225


<210>  181
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prM CDS mRNA optimized

<400>  181
gccgagguga cccgccgcgg cagcgccuac uacauguacc uggaccgcaa cgacgccggc       60

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      120

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgcg ccgugacccu gcccagccac      300

agcacccgca agcugcagac ccgcagccag accuggcugg agagccgcga guacaccaag      360

caccugaucc gcguggagaa cuggaucuuc cgcaaccccg gcuucgcccu ggccgccgcc      420

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      480

cugcugaucg cccccgccua cagc                                             504


<210>  182
<211>  1512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) E CDS mRNA optimized

<400>  182
auccgcugca ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg       60

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      120

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc      180

uacgaggcca gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc      240

uaccuggaca agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc      300

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      360

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug      420

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc      480

gacgagaacc gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug      540

ggcggcuucg gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac      600

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      660

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      720

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc      780

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      840

aagggccgcc ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug      900

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag      960

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1020

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc     1080

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1140

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1200

caccgcagcg gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc     1260

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1320

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1380

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     1440

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     1500

gccgugagcg cc                                                         1512


<210>  183
<211>  2016
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME CDS mRNA optimized

<400>  183
gccgagguga cccgccgcgg cagcgccuac uacauguacc uggaccgcaa cgacgccggc       60

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      120

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgcg ccgugacccu gcccagccac      300

agcacccgca agcugcagac ccgcagccag accuggcugg agagccgcga guacaccaag      360

caccugaucc gcguggagaa cuggaucuuc cgcaaccccg gcuucgcccu ggccgccgcc      420

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      480

cugcugaucg cccccgccua cagcauccgc ugcaucggcg ugagcaaccg cgacuucgug      540

gagggcauga gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc      600

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      660

auggccgagg ugcgcagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      720

cgcugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      780

aagcgcaccc ugguggaccg cggcuggggc aacggcugcg gccuguucgg caagggcagc      840

cuggugaccu gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc      900

gagaaccugg aguaccgcau caugcugagc gugcacggca gccagcacag cggcaugauc      960

gugaacgaca ccggccacga gaccgacgag aaccgcgcca agguggagau cacccccaac     1020

agcccccgcg ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc     1080

cgcaccggcc uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug     1140

gugcacaagg agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc     1200

accccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagcgc     1260

cagaccgugg uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc     1320

cuggaggccg agauggacgg cgccaagggc cgccugagca gcggccaccu gaagugccgc     1380

cugaagaugg acaagcugcg ccugaagggc gugagcuaca gccugugcac cgccgccuuc     1440

accuucacca agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac     1500

gccggcaccg acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug     1560

acccccgugg gccgccugau caccgccaac cccgugauca ccgagagcac cgagaacagc     1620

aagaugaugc uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc     1680

gagaagaaga ucacccacca cuggcaccgc agcggcagca ccaucggcaa ggccuucgag     1740

gccaccgugc gcggcgccaa gcgcauggcc gugcugggcg acaccgccug ggacuucggc     1800

agcgugggcg gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc     1860

uucaagagcc uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug     1920

auguggcugg gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc     1980

ggcgugcuga ucuuccugag caccgccgug agcgcc                               2016


<210>  184
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS1 CDS mRNA optimized

<400>  184
gacgugggcu gcagcgugga cuucagcaag aaggagaccc gcugcggcac cggcguguuc       60

guguacaacg acguggaggc cuggcgcgac cgcuacaagu accaccccga cagcccccgc      120

cgccuggccg ccgccgugaa gcaggccugg gaggacggca ucugcggcau cagcagcgug      180

agccgcaugg agaacaucau guggcgcagc guggagggcg agcugaacgc cauccuggag      240

gagaacggcg ugcagcugac cgugguggug ggcagcguga agaaccccau guggcgcggc      300

ccccagcgcc ugcccgugcc cgugaacgag cugccccacg gcuggaaggc cuggggcaag      360

agccacuucg ugcgcgccgc caagaccaac aacagcuucg ugguggacgg cgacacccug      420

aaggagugcc cccugaagca ccgcgccugg aacagcuucc ugguggagga ccacggcuuc      480

ggcguguucc acaccagcgu guggcugaag gugcgcgagg acuacagccu ggagugcgac      540

cccgccguga ucggcaccgc cgugaagggc aaggaggccg ugcacagcga ccugggcuac      600

uggaucgaga gcgagaagaa cgacaccugg cgccugaagc gcgcccaccu gaucgagaug      660

aagaccugcg aguggcccaa gagccacacc cuguggaccg acggcaucga ggagagcgac      720

cugaucaucc ccaagagccu ggccggcccc cugagccacc acaacacccg cgagggcuac      780

cgcacccaga ugaagggccc cuggcacagc gaggagcugg agauccgcuu cgaggagugc      840

cccggcacca aggugcacgu ggaggagacc ugcggcaccc gcggccccag ccugcgcagc      900

accaccgcca gcggccgcgu gaucgaggag uggugcugcc gcgagugcac caugcccccc      960

cugagcuucc gcgccaagga cggcugcugg uacggcaugg agauccgccc ccgcaaggag     1020

cccgagagca accuggugcg cagcauggug accgcc                               1056


<210>  185
<211>  678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS2A CDS mRNA optimized

<400>  185
ggcagcaccg accacaugga ccacuucagc cugggcgugc uggugauccu gcugauggug       60

caggagggcc ugaagaagcg caugaccacc aagaucauca ucagcaccag cauggccgug      120

cugguggcca ugauccuggg cggcuucagc augagcgacc uggccaagcu ggccauccug      180

augggcgcca ccuucgccga gaugaacacc ggcggcgacg uggcccaccu ggcccugauc      240

gccgccuuca aggugcgccc cgcccugcug gugagcuuca ucuuccgcgc caacuggacc      300

ccccgcgaga gcaugcugcu ggcccuggcc agcugccugc ugcagaccgc caucagcgcc      360

cuggagggcg accugauggu gcugaucaac ggcuucgccc uggccuggcu ggccauccgc      420

gccauggugg ugccccgcac cgacaacauc acccuggcca uccuggccgc ccugaccccc      480

cuggcccgcg gcacccugcu gguggccugg cgcgccggcc uggccaccug cggcggcuuc      540

augcugcuga gccugaaggg caagggcagc gugaagaaga accugcccuu cgugauggcc      600

cugggccuga ccgccgugcg ccugguggac cccaucaacg uggugggccu gcugcugcug      660

acccgcagcg gcaagcgc                                                    678


<210>  186
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS2B CDS mRNA optimized

<400>  186
agcuggcccc ccagcgaggu gcugaccgcc gugggccuga ucugcgcccu ggccggcggc       60

uucgccaagg ccgacaucga gauggccggc cccauggccg ccgugggccu gcugaucgug      120

agcuacgugg ugagcggcaa gagcguggac auguacaucg agcgcgccgg cgacaucacc      180

ugggagaagg acgccgaggu gaccggcaac agcccccgcc uggacguggc ccuggacgag      240

agcggcgacu ucagccuggu ggaggacgac ggccccccca ugcgcgagau cauccugaag      300

guggugcuga ugaccaucug cggcaugaac cccaucgcca uccccuucgc cgccggcgcc      360

ugguacgugu acgugaagac cggcaagcgc                                       390


<210>  187
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS3 CDS mRNA optimized

<400>  187
agcggcgccc ugugggacgu gcccgccccc aaggagguga agaagggcga gaccaccgac       60

ggcguguacc gcgugaugac ccgccgccug cugggcagca cccagguggg cgugggcgug      120

augcaggagg gcguguucca caccaugugg cacgugacca agggcagcgc ccugcgcagc      180

ggcgagggcc gccuggaccc cuacuggggc gacgugaagc aggaccuggu gagcuacugc      240

ggccccugga agcuggacgc cgccugggac ggccacagcg aggugcagcu gcuggccgug      300

ccccccggcg agcgcgcccg caacauccag acccugcccg gcaucuucaa gaccaaggac      360

ggcgacaucg gcgccguggc ccuggacuac cccgccggca ccagcggcag ccccauccug      420

gacaagugcg gccgcgugau cggccuguac ggcaacggcg uggugaucaa gaacggcagc      480

uacgugagcg ccaucaccca gggccgccgc gaggaggaga cccccgugga gugcuucgag      540

cccagcaugc ugaagaagaa gcagcugacc gugcuggacc ugcaccccgg cgccggcaag      600

acccgccgcg ugcugcccga gaucgugcgc gaggccauca agacccgccu gcgcaccgug      660

auccuggccc ccacccgcgu gguggccgcc gagauggagg aggcccugcg cggccugccc      720

gugcgcuaca ugaccaccgc cgugaacgug acccacagcg gcaccgagau cguggaccug      780

augugccacg ccaccuucac cagccgccug cugcagccca uccgcgugcc caacuacaac      840

cuguacauca uggacgaggc ccacuucacc gaccccagca gcaucgccgc ccgcggcuac      900

aucagcaccc gcguggagau gggcgaggcc gccgccaucu ucaugaccgc cacccccccc      960

ggcacccgcg acgccuuccc cgacagcaac agccccauca uggacaccga gguggaggug     1020

cccgagcgcg ccuggagcag cggcuucgac ugggugaccg acuacagcgg caagaccgug     1080

ugguucgugc ccagcgugcg caacggcaac gagaucgccg ccugccugac caaggccggc     1140

aagcgcguga uccagcugag ccgcaagacc uucgagaccg aguuccagaa gaccaagcac     1200

caggaguggg acuucguggu gaccaccgac aucagcgaga ugggcgccaa cuucaaggcc     1260

gaccgcguga ucgacagccg ccgcugccug aagcccguga uccuggacgg cgagcgcgug     1320

auccuggccg gccccaugcc cgugacccac gccagcgccg cccagcgccg cggccgcauc     1380

ggccgcaacc ccaacaagcc cggcgacgag uaccuguacg gcggcggcug cgccgagacc     1440

gacgaggacc acgcccacug gcuggaggcc cgcaugcugc uggacaacau cuaccugcag     1500

gacggccuga ucgccagccu guaccgcccc gaggccgaca agguggccgc caucgagggc     1560

gaguucaagc ugcgcaccga gcagcgcaag accuucgugg agcugaugaa gcgcggcgac     1620

cugcccgugu ggcuggccua ccagguggcc agcgccggca ucaccuacac cgaccgccgc     1680

uggugcuucg acggcaccac caacaacacc aucauggagg acagcgugcc cgccgaggug     1740

uggacccgcc acggcgagaa gcgcgugcug aagccccgcu ggauggacgc ccgcgugugc     1800

agcgaccacg ccgcccugaa gagcuucaag gaguucgccg ccggcaagcg c              1851


<210>  188
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS4A CDS mRNA optimized

<400>  188
ggcgccgccu ucggcgugau ggaggcccug ggcacccugc ccggccacau gaccgagcgc       60

uuccaggagg ccaucgacaa ccuggccgug cugaugcgcg ccgagaccgg cagccgcccc      120

uacaaggccg ccgccgccca gcugcccgag acccuggaga ccaucaugcu gcugggccug      180

cugggcaccg ugagccuggg caucuucuuc gugcugaugc gcaacaaggg caucggcaag      240

augggcuucg gcauggugac ccugggcgcc agcgccuggc ugauguggcu gagcgagauc      300

gagcccgccc gcaucgccug cgugcugauc gugguguucc ugcugcuggu ggugcugauc      360

cccgagcccg agaagcagcg cagcccccag gacaaccaga uggccaucau caucauggug      420

gccgugggcc ugcugggccu gaucaccgcc                                       450


<210>  189
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS4B CDS mRNA optimized

<400>  189
aacgagcugg gcuggcugga gcgcaccaag agcgaccuga gccaccugau gggccgccgc       60

gaggagggcg ccaccauggg cuucagcaug gacaucgacc ugcgccccgc cagcgccugg      120

gccaucuacg ccgcccugac caccuucauc acccccgccg ugcagcacgc cgugaccacc      180

agcuacaaca acuacagccu gauggccaug gccacccagg ccggcgugcu guucggcaug      240

ggcaagggca ugcccuucua cgccugggac uucggcgugc cccugcugau gaucggcugc      300

uacagccagc ugaccccccu gacccugauc guggccauca uccugcuggu ggcccacuac      360

auguaccuga uccccggccu gcaggccgcc gccgcccgcg ccgcccagaa gcgcaccgcc      420

gccggcauca ugaagaaccc cgugguggac ggcaucgugg ugaccgacau cgacaccaug      480

accaucgacc cccaggugga gaagaagaug ggccaggugc ugcugauggc cguggccgug      540

agcagcgcca uccugagccg caccgccugg ggcuggggcg aggccggcgc ccugaucacc      600

gccgccacca gcacccugug ggagggcagc cccaacaagu acuggaacag cagcaccgcc      660

accagccugu gcaacaucuu ccgcggcagc uaccuggccg gcgccagccu gaucuacacc      720

gugacccgca acgccggccu ggugaagcgc cgc                                   753


<210>  190
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS5 CDS mRNA optimized

<400>  190
ggcggcggca ccggcgagac ccugggcgag aaguggaagg cccgccugaa ccagaugagc       60

gcccuggagu ucuacagcua caagaagagc ggcaucaccg aggugugccg cgaggaggcc      120

cgccgcgccc ugaaggacgg cguggccacc ggcggccacg ccgugagccg cggcagcgcc      180

aagcugcgcu ggcuggugga gcgcggcuac cugcagcccu acggcaaggu gaucgaccug      240

ggcugcggcc gcggcggcug gagcuacuac gccgccacca uccgcaaggu gcaggaggug      300

aagggcuaca ccaagggcgg ccccggccac gaggagcccg ugcuggugca gagcuacggc      360

uggaacaucg ugcgccugaa gagcggcgug gacguguucc acauggccgc cgagcccugc      420

gacacccugc ugugcgacau cggcgagagc agcagcagcc ccgaggugga ggaggcccgc      480

acccugcgcg ugcugagcau ggugggcgac uggcuggaga agcgccccgg cgccuucugc      540

aucaaggugc ugugccccua caccagcacc augauggaga cccuggagcg ccugcagcgc      600

cgcuacggcg gcggccuggu gcgcgugccc cugagccgca acagcaccca cgagauguac      660

ugggugagcg gcgccaagag caacaccauc aagagcguga gcaccaccag ccagcugcug      720

cugggccgca uggacggccc ccgccgcccc gugaaguacg aggaggacgu gaaccugggc      780

agcggcaccc gcgccguggu gagcugcgcc gaggccccca acaugaagau caucggcaac      840

cgcaucgagc gcauccgcag cgagcacgcc gagaccuggu ucuucgacga gaaccacccc      900

uaccgcaccu gggccuacca cggcagcuac gaggccccca cccagggcag cgccagcagc      960

cugaucaacg gcguggugcg ccugcugagc aagcccuggg acguggugac cggcgugacc     1020

ggcaucgcca ugaccgacac cacccccuac ggccagcagc gcguguucaa ggagaaggug     1080

gacacccgcg ugcccgaccc ccaggagggc acccgccagg ugaugagcau ggugagcagc     1140

uggcugugga aggagcuggg caagcacaag cgcccccgcg ugugcaccaa ggaggaguuc     1200

aucaacaagg ugcgcagcaa cgccgcccug ggcgccaucu ucgaggagga gaaggagugg     1260

aagaccgccg uggaggccgu gaacgacccc cgcuucuggg cccuggugga caaggagcgc     1320

gagcaccacc ugcgcggcga gugccagagc ugcguguaca acaugauggg caagcgcgag     1380

aagaagcagg gcgaguucgg caaggccaag ggcagccgcg ccaucuggua cauguggcug     1440

ggcgcccgcu uccuggaguu cgaggcccug ggcuuccuga acgaggacca cuggaugggc     1500

cgcgagaaca gcggcggcgg cguggagggc cugggccugc agcgccuggg cuacgugcug     1560

gaggagauga gccgcauccc cggcggccgc auguacgccg acgacaccgc cggcugggac     1620

acccgcauca gccgcuucga ccuggagaac gaggcccuga ucaccaacca gauggagaag     1680

ggccaccgcg cccuggcccu ggccaucauc aaguacaccu accagaacaa gguggugaag     1740

gugcugcgcc ccgccgagaa gggcaagacc gugauggaca ucaucagccg ccaggaccag     1800

cgcggcagcg gccagguggu gaccuacgcc cugaacaccu ucaccaaccu gguggugcag     1860

cugauccgca acauggaggc cgaggaggug cuggagaugc aggaccugug gcugcugcgc     1920

cgcagcgaga aggugaccaa cuggcugcag agcaacggcu gggaccgccu gaagcgcaug     1980

gccgugagcg gcgacgacug cguggugaag cccaucgacg accgcuucgc ccacgcccug     2040

cgcuuccuga acgacauggg caaggugcgc aaggacaccc aggaguggaa gcccagcacc     2100

ggcugggaca acugggagga ggugcccuuc ugcagccacc acuucaacaa gcugcaccug     2160

aaggacggcc gcagcaucgu ggugcccugc cgccaccagg acgagcugau cggccgcgcc     2220

cgcgugagcc ccggcgccgg cuggagcauc cgcgagaccg ccugccuggc caagagcuac     2280

gcccagaugu ggcagcugcu guacuuccac cgccgcgacc ugcgccugau ggccaacgcc     2340

aucugcagca gcgugcccgu ggacugggug cccaccggcc gcaccaccug gagcauccac     2400

ggcaagggcg aguggaugac caccgaggac augcuggugg uguggaaccg cguguggauc     2460

gaggagaacg accacaugga ggacaagacc cccgugacca aguggaccga cauccccuac     2520

cugggcaagc gcgaggaccu guggugcggc agccugaucg gccaccgccc ccgcaccacc     2580

ugggccgaga acaucaagaa caccgugaac auggugcgcc gcaucaucgg cgacgaggag     2640

aaguacaugg acuaccugag cacccaggug cgcuaccugg gcgaggaggg cagcaccccc     2700

ggcgugcug                                                             2709


<210>  191
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS mRNA optimized

<400>  191
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga cccgccgcgg cagcgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggcccgccgc      360

agccgccgcg ccgugacccu gcccagccac agcacccgca agcugcagac ccgcagccag      420

accuggcugg agagccgcga guacaccaag caccugaucc gcguggagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc      600

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacccccaac agcccccgcg ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgc cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

cgccugagca gcggccaccu gaagugccgc cugaagaugg acaagcugcg ccugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gccgccugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcggcagca ccaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcgcauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  192
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS mRNA optimized

<400>  192
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga cccgccgcgg cagcgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggcccgccgc      360

agccgccgcg ccgugacccu gcccagccac agcacccgca agcugcagac ccgcagccag      420

accuggcugg agagccgcga guacaccaag caccugaucc gcguggagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc      600

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacccccaac agcccccgcg ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgc cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

cgccugagca gcggccaccu gaagugccgc cugaagaugg acaagcugcg ccugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gccgccugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcggcagca ccaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcgcauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  193
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS mRNA optimized

<400>  193
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc       60

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      120

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgcgcca agguggagau cacccccaac agcccccgcg ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagcgc cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

cgccugagca gcggccaccu gaagugccgc cugaagaugg acaagcugcg ccugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gccgccugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcaccgc     1260

agcggcagca ccaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcgcauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  194
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS mRNA optimized

<400>  194
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc       60

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      120

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgcgcca agguggagau cacccccaac agcccccgcg ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagcgc cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

cgccugagca gcggccaccu gaagugccgc cugaagaugg acaagcugcg ccugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gccgccugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcaccgc     1260

agcggcagca ccaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcgcauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  195
<211>  10269
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein CDS mRNA optimized

<400>  195
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gcggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc      360

auggccgccg aggugacccg ccgcggcagc gccuacuaca uguaccugga ccgcaacgac      420

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      480

auggaccugg gccacaccug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugauccgcgu ggagaacugg aucuuccgca accccggcuu cgcccuggcc      780

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucguga acgacaccgg ccacgagacc gacgagaacc gcgccaaggu ggagaucacc     1380

cccaacagcc cccgcgccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1440

gagccccgca ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1560

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1620

aagcgccaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1680

ggcgcccugg aggccgagau ggacggcgcc aagggccgcc ugagcagcgg ccaccugaag     1740

ugccgccuga agauggacaa gcugcgccug aagggcguga gcuacagccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1860

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1920

acccugaccc ccgugggccg ccugaucacc gccaaccccg ugaucaccga gagcaccgag     1980

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     2040

gugggcgaga agaagaucac ccaccacugg caccgcagcg gcagcaccau cggcaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc ugggcgacac cgccugggac     2160

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     2220

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     2280

cugcugaugu ggcugggccu gaacgccaag aacggcagca ucagccugau gugccuggcc     2340

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     2400

gacuucagca agaaggagac ccgcugcggc accggcgugu ucguguacaa cgacguggag     2460

gccuggcgcg accgcuacaa guaccacccc gacagccccc gccgccuggc cgccgccgug     2520

aagcaggccu gggaggacgg caucugcggc aucagcagcg ugagccgcau ggagaacauc     2580

auguggcgca gcguggaggg cgagcugaac gccauccugg aggagaacgg cgugcagcug     2640

accguggugg ugggcagcgu gaagaacccc auguggcgcg gcccccagcg ccugcccgug     2700

cccgugaacg agcugcccca cggcuggaag gccuggggca agagcuacuu cgugcgcgcc     2760

gccaagacca acaacagcuu cgugguggac ggcgacaccc ugaaggagug cccccugaag     2820

caccgcgccu ggaacagcuu ccugguggag gaccacggcu ucggcguguu ccacaccagc     2880

guguggcuga aggugcgcga ggacuacagc cuggagugcg accccgccgu gaucggcacc     2940

gccgugaagg gcaaggaggc cgugcacagc gaccugggcu acuggaucga gagcgagaag     3000

aacgacaccu ggcgccugaa gcgcgcccac cugaucgaga ugaagaccug cgaguggccc     3060

aagagccaca cccuguggac cgacggcauc gaggagagcg accugaucau ccccaagagc     3120

cuggccggcc cccugagcca ccacaacacc cgcgagggcu accgcaccca gaugaagggc     3180

cccuggcaca gcgaggagcu ggagauccgc uucgaggagu gccccggcac caaggugcac     3240

guggaggaga ccugcggcac ccgcggcccc agccugcgca gcaccaccgc cagcggccgc     3300

gugaucgagg aguggugcug ccgcgagugc accaugcccc cccugagcuu ccgcgccaag     3360

gacggcugcu gguacggcau ggagauccgc ccccgcaagg agcccgagag caaccuggug     3420

cgcagcaugg ugaccgccgg cagcaccgac cacauggacc acuucagccu gggcgugcug     3480

gugauccugc ugauggugca ggagggccug aagaagcgca ugaccaccaa gaucaucauc     3540

agcaccagca uggccgugcu gguggccaug auccugggcg gcuucagcau gagcgaccug     3600

gccaagcugg ccauccugau gggcgccacc uucgccgaga ugaacaccgg cggcgacgug     3660

gcccaccugg cccugaucgc cgccuucaag gugcgccccg cccugcuggu gagcuucauc     3720

uuccgcgcca acuggacccc ccgcgagagc augcugcugg cccuggccag cugccugcug     3780

cagaccgcca ucagcgcccu ggagggcgac cugauggugc ugaucaacgg cuucgcccug     3840

gccuggcugg ccauccgcgc caugguggug ccccgcaccg acaacaucac ccuggccauc     3900

cuggccgccc ugaccccccu ggcccgcggc acccugcugg uggccuggcg cgccggccug     3960

gccaccugcg gcggcuucau gcugcugagc cugaagggca agggcagcgu gaagaagaac     4020

cugcccuucg ugauggcccu gggccugacc gccgugcgcc ugguggaccc caucaacgug     4080

gugggccugc ugcugcugac ccgcagcggc aagcgcagcu ggccccccag cgaggugcug     4140

accgccgugg gccugaucug cgcccuggcc ggcggcuucg ccaaggccga caucgagaug     4200

gccggcccca uggccgccgu gggccugcug aucgugagcu acguggugag cggcaagagc     4260

guggacaugu acaucgagcg cgccggcgac aucaccuggg agaaggacgc cgaggugacc     4320

ggcaacagcc cccgccugga cguggcccug gacgagagcg gcgacuucag ccugguggag     4380

gacgacggcc cccccaugcg cgagaucauc cugaaggugg ugcugaugac caucugcggc     4440

augaacccca ucgccauccc cuucgccgcc ggcgccuggu acguguacgu gaagaccggc     4500

aagcgcagcg gcgcccugug ggacgugccc gcccccaagg aggugaagaa gggcgagacc     4560

accgacggcg uguaccgcgu gaugacccgc cgccugcugg gcagcaccca ggugggcgug     4620

ggcgugaugc aggagggcgu guuccacacc auguggcacg ugaccaaggg cagcgcccug     4680

cgcagcggcg agggccgccu ggaccccuac uggggcgacg ugaagcagga ccuggugagc     4740

uacugcggcc ccuggaagcu ggacgccgcc ugggacggcc acagcgaggu gcagcugcug     4800

gccgugcccc ccggcgagcg cgcccgcaac auccagaccc ugcccggcau cuucaagacc     4860

aaggacggcg acaucggcgc cguggcccug gacuaccccg ccggcaccag cggcagcccc     4920

auccuggaca agugcggccg cgugaucggc cuguacggca acggcguggu gaucaagaac     4980

ggcagcuacg ugagcgccau cacccagggc cgccgcgagg aggagacccc cguggagugc     5040

uucgagccca gcaugcugaa gaagaagcag cugaccgugc uggaccugca ccccggcgcc     5100

ggcaagaccc gccgcgugcu gcccgagauc gugcgcgagg ccaucaagac ccgccugcgc     5160

accgugaucc uggcccccac ccgcguggug gccgccgaga uggaggaggc ccugcgcggc     5220

cugcccgugc gcuacaugac caccgccgug aacgugaccc acagcggcac cgagaucgug     5280

gaccugaugu gccacgccac cuucaccagc cgccugcugc agcccauccg cgugcccaac     5340

uacaaccugu acaucaugga cgaggcccac uucaccgacc ccagcagcau cgccgcccgc     5400

ggcuacauca gcacccgcgu ggagaugggc gaggccgccg ccaucuucau gaccgccacc     5460

ccccccggca cccgcgacgc cuuccccgac agcaacagcc ccaucaugga caccgaggug     5520

gaggugcccg agcgcgccug gagcagcggc uucgacuggg ugaccgacca cagcggcaag     5580

accguguggu ucgugcccag cgugcgcaac ggcaacgaga ucgccgccug ccugaccaag     5640

gccggcaagc gcgugaucca gcugagccgc aagaccuucg agaccgaguu ccagaagacc     5700

aagcaccagg agugggacuu cguggugacc accgacauca gcgagauggg cgccaacuuc     5760

aaggccgacc gcgugaucga cagccgccgc ugccugaagc ccgugauccu ggacggcgag     5820

cgcgugaucc uggccggccc caugcccgug acccacgcca gcgccgccca gcgccgcggc     5880

cgcaucggcc gcaaccccaa caagcccggc gacgaguacc uguacggcgg cggcugcgcc     5940

gagaccgacg aggaccacgc ccacuggcug gaggcccgca ugcugcugga caacaucuac     6000

cugcaggacg gccugaucgc cagccuguac cgccccgagg ccgacaaggu ggccgccauc     6060

gagggcgagu ucaagcugcg caccgagcag cgcaagaccu ucguggagcu gaugaagcgc     6120

ggcgaccugc ccguguggcu ggccuaccag guggccagcg ccggcaucac cuacaccgac     6180

cgccgcuggu gcuucgacgg caccaccaac aacaccauca uggaggacag cgugcccgcc     6240

gaggugugga cccgccacgg cgagaagcgc gugcugaagc cccgcuggau ggacgcccgc     6300

gugugcagcg accacgccgc ccugaagagc uucaaggagu ucgccgccgg caagcgcggc     6360

gccgccuucg gcgugaugga ggcccugggc acccugcccg gccacaugac cgagcgcuuc     6420

caggaggcca ucgacaaccu ggccgugcug augcgcgccg agaccggcag ccgccccuac     6480

aaggccgccg ccgcccagcu gcccgagacc cuggagacca ucaugcugcu gggccugcug     6540

ggcaccguga gccugggcau cuucuucgug cugaugcgca acaagggcau cggcaagaug     6600

ggcuucggca uggugacccu gggcgccagc gccuggcuga uguggcugag cgagaucgag     6660

cccgcccgca ucgccugcgu gcugaucgug guguuccugc ugcugguggu gcugaucccc     6720

gagcccgaga agcagcgcag cccccaggac aaccagaugg ccaucaucau caugguggcc     6780

gugggccugc ugggccugau caccgccaac gagcugggcu ggcuggagcg caccaagagc     6840

gaccugagcc accugauggg ccgccgcgag gagggcgcca ccaucggcuu cagcauggac     6900

aucgaccugc gccccgccag cgccugggcc aucuacgccg cccugaccac cuucaucacc     6960

cccgccgugc agcacgccgu gaccaccagc uacaacaacu acagccugau ggccauggcc     7020

acccaggccg gcgugcuguu cggcaugggc aagggcaugc ccuucuacgc cugggacuuc     7080

ggcgugcccc ugcugaugau cggcugcuac agccagcuga ccccccugac ccugaucgug     7140

gccaucaucc ugcugguggc ccacuacaug uaccugaucc ccggccugca ggccgccgcc     7200

gcccgcgccg cccagaagcg caccgccgcc ggcaucauga agaaccccgu gguggacggc     7260

aucgugguga ccgacaucga caccaugacc aucgaccccc agguggagaa gaagaugggc     7320

caggugcugc ugaucgccgu ggccgugagc agcgccaucc ugagccgcac cgccuggggc     7380

uggggcgagg ccggcgcccu gaucaccgcc gccaccagca cccuguggga gggcagcccc     7440

aacaaguacu ggaacagcag caccgccacc agccugugca acaucuuccg cggcagcuac     7500

cuggccggcg ccagccugau cuacaccgug acccgcaacg ccggccuggu gaagcgccgc     7560

ggcggcggca ccggcgagac ccugggcgag aaguggaagg cccgccugaa ccagaugagc     7620

gcccuggagu ucuacagcua caagaagagc ggcaucaccg aggugugccg cgaggaggcc     7680

cgccgcgccc ugaaggacgg cguggccacc ggcggccacg ccgugagccg cggcagcgcc     7740

aagcugcgcu ggcuggugga gcgcggcuac cugcagcccu acggcaaggu gaucgaccug     7800

ggcugcggcc gcggcggcug gagcuacuac gccgccacca uccgcaaggu gcaggaggug     7860

aagggcuaca ccaagggcgg ccccggccac gaggagcccg ugcuggugca gagcuacggc     7920

uggaacaucg ugcgccugaa gagcggcgug gacguguucc acauggccgc cgagcccugc     7980

gacacccugc ugugcgacau cggcgagagc agcagcagcc ccgaggugga ggaggcccgc     8040

acccugcgcg ugcugagcau ggugggcgac uggcuggaga agcgccccgg cgccuucugc     8100

aucaaggugc ugugccccua caccagcacc augauggaga cccuggagcg ccugcagcgc     8160

cgcuacggcg gcggccuggu gcgcgugccc cugagccgca acagcaccca cgagauguac     8220

ugggugagcg gcgccaagag caacaccauc aagagcguga gcaccaccag ccagcugcug     8280

cugggccgca uggacggccc ccgccgcccc gugaaguacg aggaggacgu gaaccugggc     8340

agcggcaccc gcgccguggu gagcugcgcc gaggccccca acaugaagau caucggcaac     8400

cgcaucgagc gcauccgcag cgagcacgcc gagaccuggu ucuucgacga gaaccacccc     8460

uaccgcaccu gggccuacca cggcagcuac gaggccccca cccagggcag cgccagcagc     8520

cugaucaacg gcguggugcg ccugcugagc aagcccuggg acguggugac cggcgugacc     8580

ggcaucgcca ugaccgacac cacccccuac ggccagcagc gcguguucaa ggagaaggug     8640

gacacccgcg ugcccgaccc ccaggagggc acccgccagg ugaugagcau ggugagcagc     8700

uggcugugga aggagcuggg caagcacaag cgcccccgcg ugugcaccaa ggaggaguuc     8760

aucaacaagg ugcgcagcaa cgccgcccug ggcgccaucu ucgaggagga gaaggagugg     8820

aagaccgccg uggaggccgu gaacgacccc cgcuucuggg cccuggugga caaggagcgc     8880

gagcaccacc ugcgcggcga gugccagagc ugcguguaca acaugauggg caagcgcgag     8940

aagaagcagg gcgaguucgg caaggccaag ggcagccgcg ccaucuggua cauguggcug     9000

ggcgcccgcu uccuggaguu cgaggcccug ggcuuccuga acgaggacca cuggaugggc     9060

cgcgagaaca gcggcggcgg cguggagggc cugggccugc agcgccuggg cuacgugcug     9120

gaggagauga gccgcauccc cggcggccgc auguacgccg acgacaccgc cggcugggac     9180

acccgcauca gccgcuucga ccuggagaac gaggcccuga ucaccaacca gauggagaag     9240

ggccaccgcg cccuggcccu ggccaucauc aaguacaccu accagaacaa gguggugaag     9300

gugcugcgcc ccgccgagaa gggcaagacc gugauggaca ucaucagccg ccaggaccag     9360

cgcggcagcg gccagguggu gaccuacgcc cugaacaccu ucaccaaccu gguggugcag     9420

cugauccgca acauggaggc cgaggaggug cuggagaugc aggaccugug gcugcugcgc     9480

cgcagcgaga aggugaccaa cuggcugcag agcaacggcu gggaccgccu gaagcgcaug     9540

gccgugagcg gcgacgacug cguggugaag cccaucgacg accgcuucgc ccacgcccug     9600

cgcuuccuga acgacauggg caaggugcgc aaggacaccc aggaguggaa gcccagcacc     9660

ggcugggaca acugggagga ggugcccuuc ugcagccacc acuucaacaa gcugcaccug     9720

aaggacggcc gcagcaucgu ggugcccugc cgccaccagg acgagcugau cggccgcgcc     9780

cgcgugagcc ccggcgccgg cuggagcauc cgcgagaccg ccugccuggc caagagcuac     9840

gcccagaugu ggcagcugcu guacuuccac cgccgcgacc ugcgccugau ggccaacgcc     9900

aucugcagca gcgugcccgu ggacugggug cccaccggcc gcaccaccug gagcauccac     9960

ggcaagggcg aguggaugac caccgaggac augcuggugg uguggaaccg cguguggauc    10020

gaggagaacg accacaugga ggacaagacc cccgugacca aguggaccga cauccccuac    10080

cugggcaagc gcgaggaccu guggugcggc agccugaucg gccaccgccc ccgcaccacc    10140

ugggccgaga acaucaagaa caccgugaac auggugcgcc gcaucaucgg cgacgaggag    10200

aaguacaugg acuaccugag cacccaggug cgcuaccugg gcgaggaggg cagcaccccc    10260

ggcgugcug                                                            10269


<210>  196
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C CDS mRNA optimized

<400>  196
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gc                                                          312


<210>  197
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-ER anchor CDS mRNA optimized

<400>  197
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gcggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc      360

auggcc                                                                 366


<210>  198
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) pr CDS mRNA optimized

<400>  198
gccgagguga cccgccgcgg cagcgccuac uacauguacc uggaccgcaa cgacgccggc       60

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      120

cugggccaca ccugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgc                             279


<210>  199
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) M CDS mRNA optimized

<400>  199
gccgugaccc ugcccagcca cagcacccgc aagcugcaga cccgcagcca gaccuggcug       60

gagagccgcg aguacaccaa gcaccugauc cgcguggaga acuggaucuu ccgcaacccc      120

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      180

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagc                      225


<210>  200
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prM CDS mRNA optimized

<400>  200
gccgagguga cccgccgcgg cagcgccuac uacauguacc uggaccgcaa cgacgccggc       60

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      120

cugggccaca ccugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgcg ccgugacccu gcccagccac      300

agcacccgca agcugcagac ccgcagccag accuggcugg agagccgcga guacaccaag      360

caccugaucc gcguggagaa cuggaucuuc cgcaaccccg gcuucgcccu ggccgccgcc      420

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      480

cugcugaucg cccccgccua cagc                                             504


<210>  201
<211>  1512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) E CDS mRNA optimized

<400>  201
auccgcugca ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg       60

guggacgugg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      120

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc      180

uacgaggcca gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc      240

uaccuggaca agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc      300

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      360

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug      420

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc      480

gacgagaacc gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug      540

ggcggcuucg gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac      600

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      660

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      720

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc      780

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      840

aagggccgcc ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug      900

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag      960

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1020

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc     1080

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1140

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1200

caccgcagcg gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc     1260

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1320

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1380

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag     1440

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     1500

gccgugagcg cc                                                         1512


<210>  202
<211>  2016
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME CDS mRNA optimized

<400>  202
gccgagguga cccgccgcgg cagcgccuac uacauguacc uggaccgcaa cgacgccggc       60

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      120

cugggccaca ccugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgcg ccgugacccu gcccagccac      300

agcacccgca agcugcagac ccgcagccag accuggcugg agagccgcga guacaccaag      360

caccugaucc gcguggagaa cuggaucuuc cgcaaccccg gcuucgcccu ggccgccgcc      420

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      480

cugcugaucg cccccgccua cagcauccgc ugcaucggcg ugagcaaccg cgacuucgug      540

gagggcauga gcggcggcac cuggguggac guggugcugg agcacggcgg cugcgugacc      600

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      660

auggccgagg ugcgcagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      720

cgcugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      780

aagcgcaccc ugguggaccg cggcuggggc aacggcugcg gccuguucgg caagggcagc      840

cuggugaccu gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc      900

gagaaccugg aguaccgcau caugcugagc gugcacggca gccagcacag cggcaugauc      960

gugaacgaca ccggccacga gaccgacgag aaccgcgcca agguggagau cacccccaac     1020

agcccccgcg ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc     1080

cgcaccggcc uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug     1140

gugcacaagg agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc     1200

accccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagcgc     1260

cagaccgugg uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc     1320

cuggaggccg agauggacgg cgccaagggc cgccugagca gcggccaccu gaagugccgc     1380

cugaagaugg acaagcugcg ccugaagggc gugagcuaca gccugugcac cgccgccuuc     1440

accuucacca agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac     1500

gccggcaccg acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug     1560

acccccgugg gccgccugau caccgccaac cccgugauca ccgagagcac cgagaacagc     1620

aagaugaugc uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc     1680

gagaagaaga ucacccacca cuggcaccgc agcggcagca ccaucggcaa ggccuucgag     1740

gccaccgugc gcggcgccaa gcgcauggcc gugcugggcg acaccgccug ggacuucggc     1800

agcgugggcg gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc     1860

uucaagagcc uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug     1920

auguggcugg gccugaacgc caagaacggc agcaucagcc ugaugugccu ggcccugggc     1980

ggcgugcuga ucuuccugag caccgccgug agcgcc                               2016


<210>  203
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS1 CDS mRNA optimized

<400>  203
gacgugggcu gcagcgugga cuucagcaag aaggagaccc gcugcggcac cggcguguuc       60

guguacaacg acguggaggc cuggcgcgac cgcuacaagu accaccccga cagcccccgc      120

cgccuggccg ccgccgugaa gcaggccugg gaggacggca ucugcggcau cagcagcgug      180

agccgcaugg agaacaucau guggcgcagc guggagggcg agcugaacgc cauccuggag      240

gagaacggcg ugcagcugac cgugguggug ggcagcguga agaaccccau guggcgcggc      300

ccccagcgcc ugcccgugcc cgugaacgag cugccccacg gcuggaaggc cuggggcaag      360

agcuacuucg ugcgcgccgc caagaccaac aacagcuucg ugguggacgg cgacacccug      420

aaggagugcc cccugaagca ccgcgccugg aacagcuucc ugguggagga ccacggcuuc      480

ggcguguucc acaccagcgu guggcugaag gugcgcgagg acuacagccu ggagugcgac      540

cccgccguga ucggcaccgc cgugaagggc aaggaggccg ugcacagcga ccugggcuac      600

uggaucgaga gcgagaagaa cgacaccugg cgccugaagc gcgcccaccu gaucgagaug      660

aagaccugcg aguggcccaa gagccacacc cuguggaccg acggcaucga ggagagcgac      720

cugaucaucc ccaagagccu ggccggcccc cugagccacc acaacacccg cgagggcuac      780

cgcacccaga ugaagggccc cuggcacagc gaggagcugg agauccgcuu cgaggagugc      840

cccggcacca aggugcacgu ggaggagacc ugcggcaccc gcggccccag ccugcgcagc      900

accaccgcca gcggccgcgu gaucgaggag uggugcugcc gcgagugcac caugcccccc      960

cugagcuucc gcgccaagga cggcugcugg uacggcaugg agauccgccc ccgcaaggag     1020

cccgagagca accuggugcg cagcauggug accgcc                               1056


<210>  204
<211>  678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS2A CDS mRNA optimized

<400>  204
ggcagcaccg accacaugga ccacuucagc cugggcgugc uggugauccu gcugauggug       60

caggagggcc ugaagaagcg caugaccacc aagaucauca ucagcaccag cauggccgug      120

cugguggcca ugauccuggg cggcuucagc augagcgacc uggccaagcu ggccauccug      180

augggcgcca ccuucgccga gaugaacacc ggcggcgacg uggcccaccu ggcccugauc      240

gccgccuuca aggugcgccc cgcccugcug gugagcuuca ucuuccgcgc caacuggacc      300

ccccgcgaga gcaugcugcu ggcccuggcc agcugccugc ugcagaccgc caucagcgcc      360

cuggagggcg accugauggu gcugaucaac ggcuucgccc uggccuggcu ggccauccgc      420

gccauggugg ugccccgcac cgacaacauc acccuggcca uccuggccgc ccugaccccc      480

cuggcccgcg gcacccugcu gguggccugg cgcgccggcc uggccaccug cggcggcuuc      540

augcugcuga gccugaaggg caagggcagc gugaagaaga accugcccuu cgugauggcc      600

cugggccuga ccgccgugcg ccugguggac cccaucaacg uggugggccu gcugcugcug      660

acccgcagcg gcaagcgc                                                    678


<210>  205
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS2B CDS mRNA optimized

<400>  205
agcuggcccc ccagcgaggu gcugaccgcc gugggccuga ucugcgcccu ggccggcggc       60

uucgccaagg ccgacaucga gauggccggc cccauggccg ccgugggccu gcugaucgug      120

agcuacgugg ugagcggcaa gagcguggac auguacaucg agcgcgccgg cgacaucacc      180

ugggagaagg acgccgaggu gaccggcaac agcccccgcc uggacguggc ccuggacgag      240

agcggcgacu ucagccuggu ggaggacgac ggccccccca ugcgcgagau cauccugaag      300

guggugcuga ugaccaucug cggcaugaac cccaucgcca uccccuucgc cgccggcgcc      360

ugguacgugu acgugaagac cggcaagcgc                                       390


<210>  206
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS3 CDS mRNA optimized

<400>  206
agcggcgccc ugugggacgu gcccgccccc aaggagguga agaagggcga gaccaccgac       60

ggcguguacc gcgugaugac ccgccgccug cugggcagca cccagguggg cgugggcgug      120

augcaggagg gcguguucca caccaugugg cacgugacca agggcagcgc ccugcgcagc      180

ggcgagggcc gccuggaccc cuacuggggc gacgugaagc aggaccuggu gagcuacugc      240

ggccccugga agcuggacgc cgccugggac ggccacagcg aggugcagcu gcuggccgug      300

ccccccggcg agcgcgcccg caacauccag acccugcccg gcaucuucaa gaccaaggac      360

ggcgacaucg gcgccguggc ccuggacuac cccgccggca ccagcggcag ccccauccug      420

gacaagugcg gccgcgugau cggccuguac ggcaacggcg uggugaucaa gaacggcagc      480

uacgugagcg ccaucaccca gggccgccgc gaggaggaga cccccgugga gugcuucgag      540

cccagcaugc ugaagaagaa gcagcugacc gugcuggacc ugcaccccgg cgccggcaag      600

acccgccgcg ugcugcccga gaucgugcgc gaggccauca agacccgccu gcgcaccgug      660

auccuggccc ccacccgcgu gguggccgcc gagauggagg aggcccugcg cggccugccc      720

gugcgcuaca ugaccaccgc cgugaacgug acccacagcg gcaccgagau cguggaccug      780

augugccacg ccaccuucac cagccgccug cugcagccca uccgcgugcc caacuacaac      840

cuguacauca uggacgaggc ccacuucacc gaccccagca gcaucgccgc ccgcggcuac      900

aucagcaccc gcguggagau gggcgaggcc gccgccaucu ucaugaccgc cacccccccc      960

ggcacccgcg acgccuuccc cgacagcaac agccccauca uggacaccga gguggaggug     1020

cccgagcgcg ccuggagcag cggcuucgac ugggugaccg accacagcgg caagaccgug     1080

ugguucgugc ccagcgugcg caacggcaac gagaucgccg ccugccugac caaggccggc     1140

aagcgcguga uccagcugag ccgcaagacc uucgagaccg aguuccagaa gaccaagcac     1200

caggaguggg acuucguggu gaccaccgac aucagcgaga ugggcgccaa cuucaaggcc     1260

gaccgcguga ucgacagccg ccgcugccug aagcccguga uccuggacgg cgagcgcgug     1320

auccuggccg gccccaugcc cgugacccac gccagcgccg cccagcgccg cggccgcauc     1380

ggccgcaacc ccaacaagcc cggcgacgag uaccuguacg gcggcggcug cgccgagacc     1440

gacgaggacc acgcccacug gcuggaggcc cgcaugcugc uggacaacau cuaccugcag     1500

gacggccuga ucgccagccu guaccgcccc gaggccgaca agguggccgc caucgagggc     1560

gaguucaagc ugcgcaccga gcagcgcaag accuucgugg agcugaugaa gcgcggcgac     1620

cugcccgugu ggcuggccua ccagguggcc agcgccggca ucaccuacac cgaccgccgc     1680

uggugcuucg acggcaccac caacaacacc aucauggagg acagcgugcc cgccgaggug     1740

uggacccgcc acggcgagaa gcgcgugcug aagccccgcu ggauggacgc ccgcgugugc     1800

agcgaccacg ccgcccugaa gagcuucaag gaguucgccg ccggcaagcg c              1851


<210>  207
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS4A CDS mRNA optimized

<400>  207
ggcgccgccu ucggcgugau ggaggcccug ggcacccugc ccggccacau gaccgagcgc       60

uuccaggagg ccaucgacaa ccuggccgug cugaugcgcg ccgagaccgg cagccgcccc      120

uacaaggccg ccgccgccca gcugcccgag acccuggaga ccaucaugcu gcugggccug      180

cugggcaccg ugagccuggg caucuucuuc gugcugaugc gcaacaaggg caucggcaag      240

augggcuucg gcauggugac ccugggcgcc agcgccuggc ugauguggcu gagcgagauc      300

gagcccgccc gcaucgccug cgugcugauc gugguguucc ugcugcuggu ggugcugauc      360

cccgagcccg agaagcagcg cagcccccag gacaaccaga uggccaucau caucauggug      420

gccgugggcc ugcugggccu gaucaccgcc                                       450


<210>  208
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS4B CDS mRNA optimized

<400>  208
aacgagcugg gcuggcugga gcgcaccaag agcgaccuga gccaccugau gggccgccgc       60

gaggagggcg ccaccaucgg cuucagcaug gacaucgacc ugcgccccgc cagcgccugg      120

gccaucuacg ccgcccugac caccuucauc acccccgccg ugcagcacgc cgugaccacc      180

agcuacaaca acuacagccu gauggccaug gccacccagg ccggcgugcu guucggcaug      240

ggcaagggca ugcccuucua cgccugggac uucggcgugc cccugcugau gaucggcugc      300

uacagccagc ugaccccccu gacccugauc guggccauca uccugcuggu ggcccacuac      360

auguaccuga uccccggccu gcaggccgcc gccgcccgcg ccgcccagaa gcgcaccgcc      420

gccggcauca ugaagaaccc cgugguggac ggcaucgugg ugaccgacau cgacaccaug      480

accaucgacc cccaggugga gaagaagaug ggccaggugc ugcugaucgc cguggccgug      540

agcagcgcca uccugagccg caccgccugg ggcuggggcg aggccggcgc ccugaucacc      600

gccgccacca gcacccugug ggagggcagc cccaacaagu acuggaacag cagcaccgcc      660

accagccugu gcaacaucuu ccgcggcagc uaccuggccg gcgccagccu gaucuacacc      720

gugacccgca acgccggccu ggugaagcgc cgc                                   753


<210>  209
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS5 CDS mRNA optimized

<400>  209
ggcggcggca ccggcgagac ccugggcgag aaguggaagg cccgccugaa ccagaugagc       60

gcccuggagu ucuacagcua caagaagagc ggcaucaccg aggugugccg cgaggaggcc      120

cgccgcgccc ugaaggacgg cguggccacc ggcggccacg ccgugagccg cggcagcgcc      180

aagcugcgcu ggcuggugga gcgcggcuac cugcagcccu acggcaaggu gaucgaccug      240

ggcugcggcc gcggcggcug gagcuacuac gccgccacca uccgcaaggu gcaggaggug      300

aagggcuaca ccaagggcgg ccccggccac gaggagcccg ugcuggugca gagcuacggc      360

uggaacaucg ugcgccugaa gagcggcgug gacguguucc acauggccgc cgagcccugc      420

gacacccugc ugugcgacau cggcgagagc agcagcagcc ccgaggugga ggaggcccgc      480

acccugcgcg ugcugagcau ggugggcgac uggcuggaga agcgccccgg cgccuucugc      540

aucaaggugc ugugccccua caccagcacc augauggaga cccuggagcg ccugcagcgc      600

cgcuacggcg gcggccuggu gcgcgugccc cugagccgca acagcaccca cgagauguac      660

ugggugagcg gcgccaagag caacaccauc aagagcguga gcaccaccag ccagcugcug      720

cugggccgca uggacggccc ccgccgcccc gugaaguacg aggaggacgu gaaccugggc      780

agcggcaccc gcgccguggu gagcugcgcc gaggccccca acaugaagau caucggcaac      840

cgcaucgagc gcauccgcag cgagcacgcc gagaccuggu ucuucgacga gaaccacccc      900

uaccgcaccu gggccuacca cggcagcuac gaggccccca cccagggcag cgccagcagc      960

cugaucaacg gcguggugcg ccugcugagc aagcccuggg acguggugac cggcgugacc     1020

ggcaucgcca ugaccgacac cacccccuac ggccagcagc gcguguucaa ggagaaggug     1080

gacacccgcg ugcccgaccc ccaggagggc acccgccagg ugaugagcau ggugagcagc     1140

uggcugugga aggagcuggg caagcacaag cgcccccgcg ugugcaccaa ggaggaguuc     1200

aucaacaagg ugcgcagcaa cgccgcccug ggcgccaucu ucgaggagga gaaggagugg     1260

aagaccgccg uggaggccgu gaacgacccc cgcuucuggg cccuggugga caaggagcgc     1320

gagcaccacc ugcgcggcga gugccagagc ugcguguaca acaugauggg caagcgcgag     1380

aagaagcagg gcgaguucgg caaggccaag ggcagccgcg ccaucuggua cauguggcug     1440

ggcgcccgcu uccuggaguu cgaggcccug ggcuuccuga acgaggacca cuggaugggc     1500

cgcgagaaca gcggcggcgg cguggagggc cugggccugc agcgccuggg cuacgugcug     1560

gaggagauga gccgcauccc cggcggccgc auguacgccg acgacaccgc cggcugggac     1620

acccgcauca gccgcuucga ccuggagaac gaggcccuga ucaccaacca gauggagaag     1680

ggccaccgcg cccuggcccu ggccaucauc aaguacaccu accagaacaa gguggugaag     1740

gugcugcgcc ccgccgagaa gggcaagacc gugauggaca ucaucagccg ccaggaccag     1800

cgcggcagcg gccagguggu gaccuacgcc cugaacaccu ucaccaaccu gguggugcag     1860

cugauccgca acauggaggc cgaggaggug cuggagaugc aggaccugug gcugcugcgc     1920

cgcagcgaga aggugaccaa cuggcugcag agcaacggcu gggaccgccu gaagcgcaug     1980

gccgugagcg gcgacgacug cguggugaag cccaucgacg accgcuucgc ccacgcccug     2040

cgcuuccuga acgacauggg caaggugcgc aaggacaccc aggaguggaa gcccagcacc     2100

ggcugggaca acugggagga ggugcccuuc ugcagccacc acuucaacaa gcugcaccug     2160

aaggacggcc gcagcaucgu ggugcccugc cgccaccagg acgagcugau cggccgcgcc     2220

cgcgugagcc ccggcgccgg cuggagcauc cgcgagaccg ccugccuggc caagagcuac     2280

gcccagaugu ggcagcugcu guacuuccac cgccgcgacc ugcgccugau ggccaacgcc     2340

aucugcagca gcgugcccgu ggacugggug cccaccggcc gcaccaccug gagcauccac     2400

ggcaagggcg aguggaugac caccgaggac augcuggugg uguggaaccg cguguggauc     2460

gaggagaacg accacaugga ggacaagacc cccgugacca aguggaccga cauccccuac     2520

cugggcaagc gcgaggaccu guggugcggc agccugaucg gccaccgccc ccgcaccacc     2580

ugggccgaga acaucaagaa caccgugaac auggugcgcc gcaucaucgg cgacgaggag     2640

aaguacaugg acuaccugag cacccaggug cgcuaccugg gcgaggaggg cagcaccccc     2700

ggcgugcug                                                             2709


<210>  210
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS mRNA optimized

<400>  210
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga cccgccgcgg cagcgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ccugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggcccgccgc      360

agccgccgcg ccgugacccu gcccagccac agcacccgca agcugcagac ccgcagccag      420

accuggcugg agagccgcga guacaccaag caccugaucc gcguggagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc      600

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacccccaac agcccccgcg ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgc cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

cgccugagca gcggccaccu gaagugccgc cugaagaugg acaagcugcg ccugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gccgccugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcggcagca ccaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcgcauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacgc caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  211
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS mRNA optimized

<400>  211
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga cccgccgcgg cagcgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ccugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggcccgccgc      360

agccgccgcg ccgugacccu gcccagccac agcacccgca agcugcagac ccgcagccag      420

accuggcugg agagccgcga guacaccaag caccugaucc gcguggagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc      600

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacccccaac agcccccgcg ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgc cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

cgccugagca gcggccaccu gaagugccgc cugaagaugg acaagcugcg ccugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gccgccugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcggcagca ccaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcgcauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacgc caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  212
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS mRNA optimized

<400>  212
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc       60

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgcgcca agguggagau cacccccaac agcccccgcg ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagcgc cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

cgccugagca gcggccaccu gaagugccgc cugaagaugg acaagcugcg ccugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gccgccugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcaccgc     1260

agcggcagca ccaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcgcauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  213
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein273-719) CDS mRNA optimized

<400>  213
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc       60

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgcgcca agguggagau cacccccaac agcccccgcg ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagcgc cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

cgccugagca gcggccaccu gaagugccgc cugaagaugg acaagcugcg ccugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gccgccugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcaccgc     1260

agcggcagca ccaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcgcauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  214
<211>  10257
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein CDS mRNA optimized

<400>  214
augaagaacc ccaaggagga gauccgccgc auccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugaacccccu gggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

cgcaagcgcc gcggcgccga caccagcauc ggcaucaucg gccugcugcu gaccaccgcc      360

auggccgccg agaucacccg ccgcggcagc gccuacuaca uguaccugga ccgcagcgac      420

gccggcaagg ccaucagcuu cgccaccacc cugggcguga acaagugcca cgugcagauc      480

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugaucaaggu ggagaacugg aucuuccgca accccggcuu cgcccuggug      780

gccguggcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucacc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucggcu acgagaccga cgaggaccgc gccaaggugg aggugacccc caacagcccc     1380

cgcgccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gccccgcacc     1440

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1500

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1560

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1620

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1680

gccgagaugg acggcgccaa gggccgccug uucagcggcc accugaagug ccgccugaag     1740

auggacaagc ugcgccugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1800

accaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1860

accgacggcc ccugcaagau ccccgugcag auggccgugg acaugcagac ccugaccccc     1920

gugggccgcc ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1980

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     2040

aagaucaccc accacuggca ccgcagcggc agcaccaucg gcaaggccuu cgaggccacc     2100

gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     2160

ggcggcgugu ucaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     2220

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcuggugugg     2280

cugggccuga acaccaagaa cggcagcauc agccugaccu gccuggcccu gggcggcgug     2340

augaucuucc ugagcaccgc cgugagcgcc gacgugggcu gcagcgugga cuucagcaag     2400

aaggagaccc gcugcggcac cggcguguuc aucuacaacg acguggaggc cuggcgcgac     2460

cgcuacaagu accaccccga cagcccccgc cgccuggccg ccgccgugaa gcaggccugg     2520

gaggagggca ucugcggcau cagcagcgug agccgcaugg agaacaucau guggaagagc     2580

guggagggcg agcugaacgc cauccuggag gagaacggcg ugcagcugac cgugguggug     2640

ggcagcguga agaaccccau guggcgcggc ccccagcgcc ugcccgugcc cgugaacgag     2700

cugccccacg gcuggaaggc cuggggcaag agcuacuucg ugcgcgccgc caagaccaac     2760

aacagcuucg ugguggacgg cgacacccug aaggagugcc cccuggagca ccgcgccugg     2820

aacagcuucc ugguggagga ccacggcuuc ggcguguucc acaccagcgu guggcugaag     2880

gugcgcgagg acuacagccu ggagugcgac cccgccguga ucggcaccgc cgugaagggc     2940

cgcgaggccg cccacagcga ccugggcuac uggaucgaga gcgagaagaa cgacaccugg     3000

cgccugaagc gcgcccaccu gaucgagaug aagaccugcg aguggcccaa gagccacacc     3060

cuguggaccg acggcgugga ggagagcgac cugaucaucc ccaagagccu ggccggcccc     3120

cugagccacc acaacacccg cgagggcuac cgcacccagg ugaagggccc cuggcacagc     3180

gaggagcugg agauccgcuu cgaggagugc cccggcacca agguguacgu ggaggagacc     3240

ugcggcaccc gcggccccag ccugcgcagc accaccgcca gcggccgcgu gaucgaggag     3300

uggugcugcc gcgagugcac caugcccccc cugagcuucc gcgccaagga cggcugcugg     3360

uacggcaugg agauccgccc ccgcaaggag cccgagagca accuggugcg cagcauggug     3420

accgccggca gcaccgacca cauggaccac uucagccugg gcgugcuggu gauccugcug     3480

auggugcagg agggccugaa gaagcgcaug accaccaaga ucaucaugag caccagcaug     3540

gccgugcugg uggugaugau ccugggcggc uucagcauga gcgaccuggc caagcuggug     3600

auccugaugg gcgccaccuu cgccgagaug aacaccggcg gcgacguggc ccaccuggcc     3660

cugguggccg ccuucaaggu gcgccccgcc cugcugguga gcuucaucuu ccgcgccaac     3720

uggacccccc gcgagagcau gcugcuggcc cuggccagcu gccugcugca gaccgccauc     3780

agcgcccugg agggcgaccu gauggugcug aucaacggcu ucgcccuggc cuggcuggcc     3840

auccgcgcca uggccgugcc ccgcaccgac aacaucgccc ugcccauccu ggccgcccug     3900

accccccugg cccgcggcac ccugcuggug gccuggcgcg ccggccuggc caccugcggc     3960

ggcaucaugc ugcugagccu gaagggcaag ggcagcguga agaagaaccu gcccuucgug     4020

auggcccugg gccugaccgc cgugcgcgug guggacccca ucaacguggu gggccugcug     4080

cugcugaccc gcagcggcaa gcgcagcugg ccccccagcg aggugcugac cgccgugggc     4140

cugaucugcg cccuggccgg cggcuucgcc aaggccgaca ucgagauggc cggccccaug     4200

gccgccgugg gccugcugau cgugagcuac guggugagcg gcaagagcgu ggacauguac     4260

aucgagcgcg ccggcgacau caccugggag aaggacgccg aggugaccgg caacagcccc     4320

cgccuggacg uggcccugga cgagagcggc gacuucagcc ugguggagga ggacggcccc     4380

cccaugcgcg agaucauccu gaagguggug cugauggcca ucugcggcau gaaccccauc     4440

gccauccccu ucgccgccgg cgccugguac guguacguga agaccggcaa gcgcagcggc     4500

gcccuguggg acgugcccgc ccccaaggag gugaagaagg gcgagaccac cgacggcgug     4560

uaccgcguga ugacccgccg ccugcugggc agcacccagg ugggcguggg cgugaugcag     4620

gagggcgugu uccacaccau guggcacgug accaagggcg ccgcccugcg cagcggcgag     4680

ggccgccugg accccuacug gggcgacgug aagcaggacc uggugagcua cugcggcccc     4740

uggaagcugg acgccgccug ggacggccug agcgaggugc agcugcuggc cgugcccccc     4800

ggcgagcgcg cccgcaacau ccagacccug cccggcaucu ucaagaccaa ggacggcgac     4860

aucggcgccg uggcccugga cuaccccgcc ggcaccagcg gcagccccau ccuggacaag     4920

ugcggccgcg ugaucggccu guacggcaac ggcgugguga ucaagaacgg cagcuacgug     4980

agcgccauca cccagggcaa gcgcgaggag gagacccccg uggagugcuu cgagcccagc     5040

augcugaaga agaagcagcu gaccgugcug gaccugcacc ccggcgccgg caagacccgc     5100

cgcgugcugc ccgagaucgu gcgcgaggcc aucaagaagc gccugcgcac cgugauccug     5160

gcccccaccc gcgugguggc cgccgagaug gaggaggccc ugcgcggccu gcccgugcgc     5220

uacaugacca ccgccgugaa cgugacccac agcggcaccg agaucgugga ccugaugugc     5280

cacgccaccu ucaccagccg ccugcugcag cccauccgcg ugcccaacua caaccugaac     5340

aucauggacg aggcccacuu caccgacccc agcagcaucg ccgcccgcgg cuacaucagc     5400

acccgcgugg agaugggcga ggccgccgcc aucuucauga ccgccacccc ccccggcacc     5460

cgcgacgccu uccccgacag caacagcccc aucauggaca ccgaggugga ggugcccgag     5520

cgcgccugga gcagcggcuu cgacugggug accgaccaca gcggcaagac cgugugguuc     5580

gugcccagcg ugcgcaacgg caacgagauc gccgccugcc ugaccaaggc cggcaagcgc     5640

gugauccagc ugagccgcaa gaccuucgag accgaguucc agaagaccaa gaaccaggag     5700

ugggacuucg ugaucaccac cgacaucagc gagaugggcg ccaacuucaa ggccgaccgc     5760

gugaucgaca gccgccgcug ccugaagccc gugauccugg acggcgagcg cgugauccug     5820

gccggcccca ugcccgugac ccacgccagc gccgcccagc gccgcggccg caucggccgc     5880

aaccccaaca agcccggcga cgaguacaug uacggcggcg gcugcgccga gaccgacgag     5940

ggccacgccc acuggcugga ggcccgcaug cugcuggaca acaucuaccu gcaggacggc     6000

cugaucgcca gccuguaccg ccccgaggcc gacaaggugg ccgccaucga gggcgaguuc     6060

aagcugcgca ccgagcagcg caagaccuuc guggagcuga ugaagcgcgg cgaccugccc     6120

guguggcugg ccuaccaggu ggccagcgcc ggcaucaccu acaccgaccg ccgcuggugc     6180

uucgacggca ccaccaacaa caccaucaug gaggacagcg ugcccgccga gguguggacc     6240

aaguacggcg agaagcgcgu gcugaagccc cgcuggaugg acgcccgcgu gugcagcgac     6300

cacgccgccc ugaagagcuu caaggaguuc gccgccggca agcgcggcgc cgcccugggc     6360

gugauggagg cccugggcac ccugcccggc cacaugaccg agcgcuucca ggaggccauc     6420

gacaaccugg ccgugcugau gcgcgccgag accggcagcc gccccuacaa ggccgccgcc     6480

gcccagcugc ccgagacccu ggagaccauc augcugcugg gccugcuggg caccgugagc     6540

cugggcaucu ucuucgugcu gaugcgcaac aagggcaucg gcaagauggg cuucggcaug     6600

gugacccugg gcgccagcgc cuggcugaug uggcugagcg agaucgagcc cgcccgcauc     6660

gccugcgugc ugaucguggu guuccugcug cugguggugc ugauccccga gcccgagaag     6720

cagcgcagcc cccaggacaa ccagauggcc aucaucauca ugguggccgu gggccugcug     6780

ggccugauca ccgccaacga gcugggcugg cuggagcgca ccaagaacga caucgcccac     6840

cugaugggcc gccgcgagga gggcgccacc augggcuuca gcauggacau cgaccugcgc     6900

cccgccagcg ccugggccau cuacgccgcc cugaccaccc ugaucacccc cgccgugcag     6960

cacgccguga ccaccagcua caacaacuac agccugaugg ccauggccac ccaggccggc     7020

gugcuguucg gcaugggcaa gggcaugccc uucaugcacg gcgaccuggg cgugccccug     7080

cugaugaugg gcugcuacag ccagcugacc ccccugaccc ugaucguggc caucauccug     7140

cugguggccc acuacaugua ccugaucccc ggccugcagg ccgccgccgc ccgcgccgcc     7200

cagaagcgca ccgccgccgg caucaugaag aaccccgugg uggacggcau cguggugacc     7260

gacaucgaca ccaugaccau cgacccccag guggagaaga agaugggcca ggugcugcug     7320

aucgccgugg ccaucagcag cgccgugcug cugcgcaccg ccuggggcug gggcgaggcc     7380

ggcgcccuga ucaccgccgc caccagcacc cugugggagg gcagccccaa caaguacugg     7440

aacagcagca ccgccaccag ccugugcaac aucuuccgcg gcagcuaccu ggccggcgcc     7500

agccugaucu acaccgugac ccgcaacgcc ggccugguga agcgccgcgg cggcggcacc     7560

ggcgagaccc ugggcgagaa guggaaggcc cgccugaacc agaugagcgc ccuggaguuc     7620

uacagcuaca agaagagcgg caucaccgag gugugccgcg aggaggcccg ccgcgcccug     7680

aaggacggcg uggccaccgg cggccacgcc gugagccgcg gcagcgccaa gauccgcugg     7740

cuggaggagc gcggcuaccu gcagcccuac ggcaaggugg uggaccuggg cugcggccgc     7800

ggcggcugga gcuacuacgc cgccaccauc cgcaaggugc aggaggugcg cggcuacacc     7860

aagggcggcc ccggccacga ggagcccaug cuggugcaga gcuacggcug gaacaucgug     7920

cgccugaaga gcggcgugga cguguuccac auggccgccg agcccugcga cacccugcug     7980

ugcgacaucg gcgagagcag cagcagcccc gagguggagg agacccgcac ccugcgcgug     8040

cugagcaugg ugggcgacug gcuggagaag cgccccggcg ccuucugcau caaggugcug     8100

ugccccuaca ccagcaccau gauggagacc auggagcgcc ugcagcgccg ccacggcggc     8160

ggccuggugc gcgugccccu gugccgcaac agcacccacg agauguacug ggugagcggc     8220

gccaagagca acaucaucaa gagcgugagc accaccagcc agcugcugcu gggccgcaug     8280

gacggccccc gccgccccgu gaaguacgag gaggacguga accugggcag cggcacccgc     8340

gccguggcca gcugcgccga ggcccccaac augaagauca ucggccgccg caucgagcgc     8400

auccgcaacg agcacgccga gaccugguuc cuggacgaga accaccccua ccgcaccugg     8460

gccuaccacg gcagcuacga ggcccccacc cagggcagcg ccagcagccu ggugaacggc     8520

guggugcgcc ugcugagcaa gcccugggac guggugaccg gcgugaccgg caucgccaug     8580

accgacacca cccccuacgg ccagcagcgc guguucaagg agaaggugga cacccgcgug     8640

cccgaccccc aggagggcac ccgccaggug augaacaucg ugagcagcug gcuguggaag     8700

gagcugggca agcgcaagcg cccccgcgug ugcaccaagg aggaguucau caacaaggug     8760

cgcagcaacg ccgcccuggg cgccaucuuc gaggaggaga aggaguggaa gaccgccgug     8820

gaggccguga acgacccccg cuucugggcc cugguggacc gcgagcgcga gcaccaccug     8880

cgcggcgagu gccacagcug cguguacaac augaugggca agcgcgagaa gaagcagggc     8940

gaguucggca aggccaaggg cagccgcgcc aucugguaca uguggcuggg cgcccgcuuc     9000

cuggaguucg aggcccuggg cuuccugaac gaggaccacu ggaugggccg cgagaacagc     9060

ggcggcggcg uggagggccu gggccugcag cgccugggcu acauccugga ggagaugaac     9120

cgcgcccccg gcggcaagau guacgccgac gacaccgccg gcugggacac ccgcaucagc     9180

aaguucgacc uggagaacga ggcccugauc accaaccaga uggaggaggg ccaccgcacc     9240

cuggcccugg ccgugaucaa guacaccuac cagaacaagg uggugaaggu gcugcgcccc     9300

gccgagggcg gcaagaccgu gauggacauc aucagccgcc aggaccagcg cggcagcggc     9360

caggugguga ccuacgcccu gaacaccuuc accaaccugg uggugcagcu gauccgcaac     9420

auggaggccg aggaggugcu ggagaugcag gaccuguggc ugcugcgcaa gcccgagaag     9480

gugacccgcu ggcugcagag caacggcugg gaccgccuga agcgcauggc cgugagcggc     9540

gacgacugcg uggugaagcc caucgacgac cgcuucgccc acgcccugcg cuuccugaac     9600

gacaugggca aggugcgcaa ggacacccag gaguggaagc ccagcaccgg cuggagcaac     9660

ugggaggagg ugcccuucug cagccaccac uucaacaagc uguaccugaa ggacggccgc     9720

agcaucgugg ugcccugccg ccaccaggac gagcugaucg gccgcgcccg cgugagcccc     9780

ggcgccggcu ggagcauccg cgagaccgcc ugccuggcca agagcuacgc ccagaugugg     9840

cagcugcugu acuuccaccg ccgcgaccug cgccugaugg ccaacgccau cugcagcgcc     9900

gugcccgugg acugggugcc caccggccgc accaccugga gcauccacgg caagggcgag     9960

uggaugacca ccgaggacau gcugauggug uggaaccgcg uguggaucga ggagaacgac    10020

cacauggagg acaagacccc cgugaccaag uggaccgaca uccccuaccu gggcaagcgc    10080

gaggaccugu ggugcggcag ccugaucggc caccgccccc gcaccaccug ggccgagaac    10140

aucaaggaca ccgugaacau ggugcgccgc aucaucggcg acgaggagaa guacauggac    10200

uaccugagca cccaggugcg cuaccugggc gaggagggca gcacccccgg cgugcug       10257


<210>  215
<211>  312
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C CDS mRNA optimized

<400>  215
augaagaacc ccaaggagga gauccgccgc auccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugaacccccu gggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

cgcaagcgcc gc                                                          312


<210>  216
<211>  366
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-ER anchor CDS mRNA optimized

<400>  216
augaagaacc ccaaggagga gauccgccgc auccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugaacccccu gggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

cgcaagcgcc gcggcgccga caccagcauc ggcaucaucg gccugcugcu gaccaccgcc      360

auggcc                                                                 366


<210>  217
<211>  279
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) pr CDS mRNA optimized

<400>  217
gccgagauca cccgccgcgg cagcgccuac uacauguacc uggaccgcag cgacgccggc       60

aaggccauca gcuucgccac cacccugggc gugaacaagu gccacgugca gaucauggac      120

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgc                             279


<210>  218
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) M CDS mRNA optimized

<400>  218
gccgugaccc ugcccagcca cagcacccgc aagcugcaga cccgcagcca gaccuggcug       60

gagagccgcg aguacaccaa gcaccugauc aagguggaga acuggaucuu ccgcaacccc      120

ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg gcagcagcac cagccagaag      180

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagc                      225


<210>  219
<211>  504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prM CDS mRNA optimized

<400>  219
gccgagauca cccgccgcgg cagcgccuac uacauguacc uggaccgcag cgacgccggc       60

aaggccauca gcuucgccac cacccugggc gugaacaagu gccacgugca gaucauggac      120

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgcg ccgugacccu gcccagccac      300

agcacccgca agcugcagac ccgcagccag accuggcugg agagccgcga guacaccaag      360

caccugauca agguggagaa cuggaucuuc cgcaaccccg gcuucgcccu gguggccgug      420

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      480

cugcugaucg cccccgccua cagc                                             504


<210>  220
<211>  1500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) E CDS mRNA optimized

<400>  220
auccgcugca ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg       60

guggacgugg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      120

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc      180

uacgaggcca gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc      240

uaccuggaca agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc      300

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc      360

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug      420

cugagcgugc acggcagcca gcacagcggc augaucggcu acgagaccga cgaggaccgc      480

gccaaggugg aggugacccc caacagcccc cgcgccgagg ccacccuggg cggcuucggc      540

agccugggcc uggacugcga gccccgcacc ggccuggacu ucagcgaccu guacuaccug      600

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc      660

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag      720

uucaaggacg cccacgccaa gcgccagacc gugguggugc ugggcagcca ggagggcgcc      780

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggccgccug      840

uucagcggcc accugaagug ccgccugaag auggacaagc ugcgccugaa gggcgugagc      900

uacagccugu gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc      960

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag     1020

auggccgugg acaugcagac ccugaccccc gugggccgcc ugaucaccgc caaccccgug     1080

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1140

agcuacaucg ugaucggcgu gggcgacaag aagaucaccc accacuggca ccgcagcggc     1200

agcaccaucg gcaaggccuu cgaggccacc gugcgcggcg ccaagcgcau ggccgugcug     1260

ggcgacaccg ccugggacuu cggcagcgug ggcggcgugu ucaacagccu gggcaagggc     1320

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1380

cagauccuga ucggcacccu gcuggugugg cugggccuga acaccaagaa cggcagcauc     1440

agccugaccu gccuggcccu gggcggcgug augaucuucc ugagcaccgc cgugagcgcc     1500


<210>  221
<211>  2004
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME CDS mRNA optimized

<400>  221
gccgagauca cccgccgcgg cagcgccuac uacauguacc uggaccgcag cgacgccggc       60

aaggccauca gcuucgccac cacccugggc gugaacaagu gccacgugca gaucauggac      120

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      180

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      240

ugccaccaca agaagggcga ggcccgccgc agccgccgcg ccgugacccu gcccagccac      300

agcacccgca agcugcagac ccgcagccag accuggcugg agagccgcga guacaccaag      360

caccugauca agguggagaa cuggaucuuc cgcaaccccg gcuucgcccu gguggccgug      420

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      480

cugcugaucg cccccgccua cagcauccgc ugcaucggcg ugagcaaccg cgacuucgug      540

gagggcauga gcggcggcac cuggguggac guggugcugg agcacggcgg cugcgugacc      600

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      660

auggccgagg ugcgcagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      720

cgcugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      780

aagcgcaccc ugguggaccg cggcuggggc aacggcugcg gccuguucgg caagggcagc      840

cuggugaccu gcgccaaguu caccugcagc aagaagauga ccggcaagag cauccagccc      900

gagaaccugg aguaccgcau caugcugagc gugcacggca gccagcacag cggcaugauc      960

ggcuacgaga ccgacgagga ccgcgccaag guggagguga cccccaacag cccccgcgcc     1020

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug     1080

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1140

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1200

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug     1260

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1320

auggacggcg ccaagggccg ccuguucagc ggccaccuga agugccgccu gaagauggac     1380

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1440

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1500

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1560

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1620

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1680

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1740

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1800

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1860

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     1920

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     1980

uuccugagca ccgccgugag cgcc                                            2004


<210>  222
<211>  1056
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS1 CDS mRNA optimized

<400>  222
gacgugggcu gcagcgugga cuucagcaag aaggagaccc gcugcggcac cggcguguuc       60

aucuacaacg acguggaggc cuggcgcgac cgcuacaagu accaccccga cagcccccgc      120

cgccuggccg ccgccgugaa gcaggccugg gaggagggca ucugcggcau cagcagcgug      180

agccgcaugg agaacaucau guggaagagc guggagggcg agcugaacgc cauccuggag      240

gagaacggcg ugcagcugac cgugguggug ggcagcguga agaaccccau guggcgcggc      300

ccccagcgcc ugcccgugcc cgugaacgag cugccccacg gcuggaaggc cuggggcaag      360

agcuacuucg ugcgcgccgc caagaccaac aacagcuucg ugguggacgg cgacacccug      420

aaggagugcc cccuggagca ccgcgccugg aacagcuucc ugguggagga ccacggcuuc      480

ggcguguucc acaccagcgu guggcugaag gugcgcgagg acuacagccu ggagugcgac      540

cccgccguga ucggcaccgc cgugaagggc cgcgaggccg cccacagcga ccugggcuac      600

uggaucgaga gcgagaagaa cgacaccugg cgccugaagc gcgcccaccu gaucgagaug      660

aagaccugcg aguggcccaa gagccacacc cuguggaccg acggcgugga ggagagcgac      720

cugaucaucc ccaagagccu ggccggcccc cugagccacc acaacacccg cgagggcuac      780

cgcacccagg ugaagggccc cuggcacagc gaggagcugg agauccgcuu cgaggagugc      840

cccggcacca agguguacgu ggaggagacc ugcggcaccc gcggccccag ccugcgcagc      900

accaccgcca gcggccgcgu gaucgaggag uggugcugcc gcgagugcac caugcccccc      960

cugagcuucc gcgccaagga cggcugcugg uacggcaugg agauccgccc ccgcaaggag     1020

cccgagagca accuggugcg cagcauggug accgcc                               1056


<210>  223
<211>  678
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS2A CDS mRNA optimized

<400>  223
ggcagcaccg accacaugga ccacuucagc cugggcgugc uggugauccu gcugauggug       60

caggagggcc ugaagaagcg caugaccacc aagaucauca ugagcaccag cauggccgug      120

cuggugguga ugauccuggg cggcuucagc augagcgacc uggccaagcu ggugauccug      180

augggcgcca ccuucgccga gaugaacacc ggcggcgacg uggcccaccu ggcccuggug      240

gccgccuuca aggugcgccc cgcccugcug gugagcuuca ucuuccgcgc caacuggacc      300

ccccgcgaga gcaugcugcu ggcccuggcc agcugccugc ugcagaccgc caucagcgcc      360

cuggagggcg accugauggu gcugaucaac ggcuucgccc uggccuggcu ggccauccgc      420

gccauggccg ugccccgcac cgacaacauc gcccugccca uccuggccgc ccugaccccc      480

cuggcccgcg gcacccugcu gguggccugg cgcgccggcc uggccaccug cggcggcauc      540

augcugcuga gccugaaggg caagggcagc gugaagaaga accugcccuu cgugauggcc      600

cugggccuga ccgccgugcg cgugguggac cccaucaacg uggugggccu gcugcugcug      660

acccgcagcg gcaagcgc                                                    678


<210>  224
<211>  390
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS2B CDS mRNA optimized

<400>  224
agcuggcccc ccagcgaggu gcugaccgcc gugggccuga ucugcgcccu ggccggcggc       60

uucgccaagg ccgacaucga gauggccggc cccauggccg ccgugggccu gcugaucgug      120

agcuacgugg ugagcggcaa gagcguggac auguacaucg agcgcgccgg cgacaucacc      180

ugggagaagg acgccgaggu gaccggcaac agcccccgcc uggacguggc ccuggacgag      240

agcggcgacu ucagccuggu ggaggaggac ggccccccca ugcgcgagau cauccugaag      300

guggugcuga uggccaucug cggcaugaac cccaucgcca uccccuucgc cgccggcgcc      360

ugguacgugu acgugaagac cggcaagcgc                                       390


<210>  225
<211>  1851
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS3 CDS mRNA optimized

<400>  225
agcggcgccc ugugggacgu gcccgccccc aaggagguga agaagggcga gaccaccgac       60

ggcguguacc gcgugaugac ccgccgccug cugggcagca cccagguggg cgugggcgug      120

augcaggagg gcguguucca caccaugugg cacgugacca agggcgccgc ccugcgcagc      180

ggcgagggcc gccuggaccc cuacuggggc gacgugaagc aggaccuggu gagcuacugc      240

ggccccugga agcuggacgc cgccugggac ggccugagcg aggugcagcu gcuggccgug      300

ccccccggcg agcgcgcccg caacauccag acccugcccg gcaucuucaa gaccaaggac      360

ggcgacaucg gcgccguggc ccuggacuac cccgccggca ccagcggcag ccccauccug      420

gacaagugcg gccgcgugau cggccuguac ggcaacggcg uggugaucaa gaacggcagc      480

uacgugagcg ccaucaccca gggcaagcgc gaggaggaga cccccgugga gugcuucgag      540

cccagcaugc ugaagaagaa gcagcugacc gugcuggacc ugcaccccgg cgccggcaag      600

acccgccgcg ugcugcccga gaucgugcgc gaggccauca agaagcgccu gcgcaccgug      660

auccuggccc ccacccgcgu gguggccgcc gagauggagg aggcccugcg cggccugccc      720

gugcgcuaca ugaccaccgc cgugaacgug acccacagcg gcaccgagau cguggaccug      780

augugccacg ccaccuucac cagccgccug cugcagccca uccgcgugcc caacuacaac      840

cugaacauca uggacgaggc ccacuucacc gaccccagca gcaucgccgc ccgcggcuac      900

aucagcaccc gcguggagau gggcgaggcc gccgccaucu ucaugaccgc cacccccccc      960

ggcacccgcg acgccuuccc cgacagcaac agccccauca uggacaccga gguggaggug     1020

cccgagcgcg ccuggagcag cggcuucgac ugggugaccg accacagcgg caagaccgug     1080

ugguucgugc ccagcgugcg caacggcaac gagaucgccg ccugccugac caaggccggc     1140

aagcgcguga uccagcugag ccgcaagacc uucgagaccg aguuccagaa gaccaagaac     1200

caggaguggg acuucgugau caccaccgac aucagcgaga ugggcgccaa cuucaaggcc     1260

gaccgcguga ucgacagccg ccgcugccug aagcccguga uccuggacgg cgagcgcgug     1320

auccuggccg gccccaugcc cgugacccac gccagcgccg cccagcgccg cggccgcauc     1380

ggccgcaacc ccaacaagcc cggcgacgag uacauguacg gcggcggcug cgccgagacc     1440

gacgagggcc acgcccacug gcuggaggcc cgcaugcugc uggacaacau cuaccugcag     1500

gacggccuga ucgccagccu guaccgcccc gaggccgaca agguggccgc caucgagggc     1560

gaguucaagc ugcgcaccga gcagcgcaag accuucgugg agcugaugaa gcgcggcgac     1620

cugcccgugu ggcuggccua ccagguggcc agcgccggca ucaccuacac cgaccgccgc     1680

uggugcuucg acggcaccac caacaacacc aucauggagg acagcgugcc cgccgaggug     1740

uggaccaagu acggcgagaa gcgcgugcug aagccccgcu ggauggacgc ccgcgugugc     1800

agcgaccacg ccgcccugaa gagcuucaag gaguucgccg ccggcaagcg c              1851


<210>  226
<211>  450
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS4A CDS mRNA optimized

<400>  226
ggcgccgccc ugggcgugau ggaggcccug ggcacccugc ccggccacau gaccgagcgc       60

uuccaggagg ccaucgacaa ccuggccgug cugaugcgcg ccgagaccgg cagccgcccc      120

uacaaggccg ccgccgccca gcugcccgag acccuggaga ccaucaugcu gcugggccug      180

cugggcaccg ugagccuggg caucuucuuc gugcugaugc gcaacaaggg caucggcaag      240

augggcuucg gcauggugac ccugggcgcc agcgccuggc ugauguggcu gagcgagauc      300

gagcccgccc gcaucgccug cgugcugauc gugguguucc ugcugcuggu ggugcugauc      360

cccgagcccg agaagcagcg cagcccccag gacaaccaga uggccaucau caucauggug      420

gccgugggcc ugcugggccu gaucaccgcc                                       450


<210>  227
<211>  753
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS4B CDS mRNA optimized

<400>  227
aacgagcugg gcuggcugga gcgcaccaag aacgacaucg cccaccugau gggccgccgc       60

gaggagggcg ccaccauggg cuucagcaug gacaucgacc ugcgccccgc cagcgccugg      120

gccaucuacg ccgcccugac cacccugauc acccccgccg ugcagcacgc cgugaccacc      180

agcuacaaca acuacagccu gauggccaug gccacccagg ccggcgugcu guucggcaug      240

ggcaagggca ugcccuucau gcacggcgac cugggcgugc cccugcugau gaugggcugc      300

uacagccagc ugaccccccu gacccugauc guggccauca uccugcuggu ggcccacuac      360

auguaccuga uccccggccu gcaggccgcc gccgcccgcg ccgcccagaa gcgcaccgcc      420

gccggcauca ugaagaaccc cgugguggac ggcaucgugg ugaccgacau cgacaccaug      480

accaucgacc cccaggugga gaagaagaug ggccaggugc ugcugaucgc cguggccauc      540

agcagcgccg ugcugcugcg caccgccugg ggcuggggcg aggccggcgc ccugaucacc      600

gccgccacca gcacccugug ggagggcagc cccaacaagu acuggaacag cagcaccgcc      660

accagccugu gcaacaucuu ccgcggcagc uaccuggccg gcgccagccu gaucuacacc      720

gugacccgca acgccggccu ggugaagcgc cgc                                   753


<210>  228
<211>  2709
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS5 CDS mRNA optimized

<400>  228
ggcggcggca ccggcgagac ccugggcgag aaguggaagg cccgccugaa ccagaugagc       60

gcccuggagu ucuacagcua caagaagagc ggcaucaccg aggugugccg cgaggaggcc      120

cgccgcgccc ugaaggacgg cguggccacc ggcggccacg ccgugagccg cggcagcgcc      180

aagauccgcu ggcuggagga gcgcggcuac cugcagcccu acggcaaggu gguggaccug      240

ggcugcggcc gcggcggcug gagcuacuac gccgccacca uccgcaaggu gcaggaggug      300

cgcggcuaca ccaagggcgg ccccggccac gaggagccca ugcuggugca gagcuacggc      360

uggaacaucg ugcgccugaa gagcggcgug gacguguucc acauggccgc cgagcccugc      420

gacacccugc ugugcgacau cggcgagagc agcagcagcc ccgaggugga ggagacccgc      480

acccugcgcg ugcugagcau ggugggcgac uggcuggaga agcgccccgg cgccuucugc      540

aucaaggugc ugugccccua caccagcacc augauggaga ccauggagcg ccugcagcgc      600

cgccacggcg gcggccuggu gcgcgugccc cugugccgca acagcaccca cgagauguac      660

ugggugagcg gcgccaagag caacaucauc aagagcguga gcaccaccag ccagcugcug      720

cugggccgca uggacggccc ccgccgcccc gugaaguacg aggaggacgu gaaccugggc      780

agcggcaccc gcgccguggc cagcugcgcc gaggccccca acaugaagau caucggccgc      840

cgcaucgagc gcauccgcaa cgagcacgcc gagaccuggu uccuggacga gaaccacccc      900

uaccgcaccu gggccuacca cggcagcuac gaggccccca cccagggcag cgccagcagc      960

cuggugaacg gcguggugcg ccugcugagc aagcccuggg acguggugac cggcgugacc     1020

ggcaucgcca ugaccgacac cacccccuac ggccagcagc gcguguucaa ggagaaggug     1080

gacacccgcg ugcccgaccc ccaggagggc acccgccagg ugaugaacau cgugagcagc     1140

uggcugugga aggagcuggg caagcgcaag cgcccccgcg ugugcaccaa ggaggaguuc     1200

aucaacaagg ugcgcagcaa cgccgcccug ggcgccaucu ucgaggagga gaaggagugg     1260

aagaccgccg uggaggccgu gaacgacccc cgcuucuggg cccuggugga ccgcgagcgc     1320

gagcaccacc ugcgcggcga gugccacagc ugcguguaca acaugauggg caagcgcgag     1380

aagaagcagg gcgaguucgg caaggccaag ggcagccgcg ccaucuggua cauguggcug     1440

ggcgcccgcu uccuggaguu cgaggcccug ggcuuccuga acgaggacca cuggaugggc     1500

cgcgagaaca gcggcggcgg cguggagggc cugggccugc agcgccuggg cuacauccug     1560

gaggagauga accgcgcccc cggcggcaag auguacgccg acgacaccgc cggcugggac     1620

acccgcauca gcaaguucga ccuggagaac gaggcccuga ucaccaacca gauggaggag     1680

ggccaccgca cccuggcccu ggccgugauc aaguacaccu accagaacaa gguggugaag     1740

gugcugcgcc ccgccgaggg cggcaagacc gugauggaca ucaucagccg ccaggaccag     1800

cgcggcagcg gccagguggu gaccuacgcc cugaacaccu ucaccaaccu gguggugcag     1860

cugauccgca acauggaggc cgaggaggug cuggagaugc aggaccugug gcugcugcgc     1920

aagcccgaga aggugacccg cuggcugcag agcaacggcu gggaccgccu gaagcgcaug     1980

gccgugagcg gcgacgacug cguggugaag cccaucgacg accgcuucgc ccacgcccug     2040

cgcuuccuga acgacauggg caaggugcgc aaggacaccc aggaguggaa gcccagcacc     2100

ggcuggagca acugggagga ggugcccuuc ugcagccacc acuucaacaa gcuguaccug     2160

aaggacggcc gcagcaucgu ggugcccugc cgccaccagg acgagcugau cggccgcgcc     2220

cgcgugagcc ccggcgccgg cuggagcauc cgcgagaccg ccugccuggc caagagcuac     2280

gcccagaugu ggcagcugcu guacuuccac cgccgcgacc ugcgccugau ggccaacgcc     2340

aucugcagcg ccgugcccgu ggacugggug cccaccggcc gcaccaccug gagcauccac     2400

ggcaagggcg aguggaugac caccgaggac augcugaugg uguggaaccg cguguggauc     2460

gaggagaacg accacaugga ggacaagacc cccgugacca aguggaccga cauccccuac     2520

cugggcaagc gcgaggaccu guggugcggc agccugaucg gccaccgccc ccgcaccacc     2580

ugggccgaga acaucaagga caccgugaac auggugcgcc gcaucaucgg cgacgaggag     2640

aaguacaugg acuaccugag cacccaggug cgcuaccugg gcgaggaggg cagcaccccc     2700

ggcgugcug                                                             2709


<210>  229
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS mRNA optimized

<400>  229
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca cccgccgcgg cagcgccuac      120

uacauguacc uggaccgcag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggcccgccgc      360

agccgccgcg ccgugacccu gcccagccac agcacccgca agcugcagac ccgcagccag      420

accuggcugg agagccgcga guacaccaag caccugauca agguggagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc      600

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag     1080

guggagguga cccccaacag cccccgcgcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagccccg caccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagcgcca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggccg ccuguucagc     1440

ggccaccuga agugccgccu gaagauggac aagcugcgcc ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1620

guggacaugc agacccugac ccccgugggc cgccugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcaccgcag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugcgc ggcgccaagc gcauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug     2040

accugccugg cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcagcg uggacuucag caag                                            2124


<210>  230
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS mRNA optimized

<400>  230
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca cccgccgcgg cagcgccuac      120

uacauguacc uggaccgcag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggcccgccgc      360

agccgccgcg ccgugacccu gcccagccac agcacccgca agcugcagac ccgcagccag      420

accuggcugg agagccgcga guacaccaag caccugauca agguggagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc      600

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag     1080

guggagguga cccccaacag cccccgcgcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagccccg caccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagcgcca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggccg ccuguucagc     1440

ggccaccuga agugccgccu gaagauggac aagcugcgcc ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1620

guggacaugc agacccugac ccccgugggc cgccugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcaccgcag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugcgc ggcgccaagc gcauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug     2040

accugccugg cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcagcg uggacuucag caag                                            2124


<210>  231
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS mRNA optimized

<400>  231
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc       60

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc      480

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag      540

guggagguga cccccaacag cccccgcgcc gaggccaccc ugggcggcuu cggcagccug      600

ggccuggacu gcgagccccg caccggccug gacuucagcg accuguacua ccugaccaug      660

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac      720

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag      780

gacgcccacg ccaagcgcca gaccguggug gugcugggca gccaggaggg cgccgugcac      840

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggccg ccuguucagc      900

ggccaccuga agugccgccu gaagauggac aagcugcgcc ugaagggcgu gagcuacagc      960

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1020

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1080

guggacaugc agacccugac ccccgugggc cgccugauca ccgccaaccc cgugaucacc     1140

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1200

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcaccgcag cggcagcacc     1260

aucggcaagg ccuucgaggc caccgugcgc ggcgccaagc gcauggccgu gcugggcgac     1320

accgccuggg acuucggcag c                                               1341


<210>  232
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS mRNA optimized

<400>  232
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcauccgc       60

ugcaucggcg ugagcaaccg cgacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg cggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugagc      480

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag      540

guggagguga cccccaacag cccccgcgcc gaggccaccc ugggcggcuu cggcagccug      600

ggccuggacu gcgagccccg caccggccug gacuucagcg accuguacua ccugaccaug      660

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac      720

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag      780

gacgcccacg ccaagcgcca gaccguggug gugcugggca gccaggaggg cgccgugcac      840

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggccg ccuguucagc      900

ggccaccuga agugccgccu gaagauggac aagcugcgcc ugaagggcgu gagcuacagc      960

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1020

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1080

guggacaugc agacccugac ccccgugggc cgccugauca ccgccaaccc cgugaucacc     1140

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1200

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcaccgcag cggcagcacc     1260

aucggcaagg ccuucgaggc caccgugcgc ggcgccaagc gcauggccgu gcugggcgac     1320

accgccuggg acuucggcag c                                               1341


<210>  233
<211>  10489
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein mRNA Design 1 wild
       type

<400>  233
gggagaaagc uuaccaugaa aaacccaaaa aagaaauccg gaggauuccg gauugucaau       60

augcuaaaac gcggaguagc ccgugugagc cccuuugggg gcuugaagag gcugccagcc      120

ggacuucugc ugggucaugg gcccaucagg auggucuugg caauucuagc cuuuuugaga      180

uucacggcaa ucaagccauc acugggucuc aucaauagau gggguucagu ggggaaaaaa      240

gaggcuaugg aaauaauaaa gaaguucaag aaagaucugg cugccaugcu gagaauaauc      300

aaugcuagga aggagaagaa gagacggggc gcagauacua gugucggaau uguuggccuc      360

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      420

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      480

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      540

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      600

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      660

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      720

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      780

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      840

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      900

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      960

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug     1020

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua     1080

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa     1140

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu     1200

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug     1260

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1320

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1380

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1440

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1500

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1560

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1620

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1680

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1740

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1800

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1860

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1920

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1980

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     2040

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     2100

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     2160

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     2220

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     2280

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2340

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2400

guggggugcu cgguggacuu cucaaagaag gagacgagau gcgguacagg gguguucguc     2460

uauaacgacg uugaagccug gagggacagg uacaaguacc auccugacuc cccccguaga     2520

uuggcagcag cagucaagca agccugggaa gaugguaucu gcgggaucuc cucuguuuca     2580

agaauggaaa acaucaugug gagaucagua gaaggggagc ucaacgcaau ccuggaagag     2640

aauggaguuc aacugacggu cguuguggga ucuguaaaaa accccaugug gagaggucca     2700

cagagauugc ccgugccugu gaacgagcug ccccacggcu ggaaggcuug ggggaaaucg     2760

cacuucguca gagcagcaaa gacaaauaac agcuuugucg uggaugguga cacacugaag     2820

gaaugcccac ucaaacauag agcauggaac agcuuucuug uggaggauca uggguucggg     2880

guauuucaca cuagugucug gcucaagguu agagaagauu auucauuaga gugugaucca     2940

gccguuauug gaacagcugu uaagggaaag gaggcuguac acagugaucu aggcuacugg     3000

auugagagug agaagaauga cacauggagg cugaagaggg cccaucugau cgagaugaaa     3060

acaugugaau ggccaaaguc ccacacauug uggacagaug gaauagaaga gagugaucug     3120

aucauaccca agucuuuagc ugggccacuc agccaucaca auaccagaga gggcuacagg     3180

acccaaauga aagggccaug gcacagugaa gagcuugaaa uucgguuuga ggaaugccca     3240

ggcacuaagg uccacgugga ggaaacaugu ggaacaagag gaccaucucu gagaucaacc     3300

acugcaagcg gaagggugau cgaggaaugg ugcugcaggg agugcacaau gcccccacug     3360

ucguuccggg cuaaagaugg cuguugguau ggaauggaga uaaggcccag gaaagaacca     3420

gaaagcaacu uaguaagguc aauggugacu gcaggaucaa cugaucacau ggaucacuuc     3480

ucccuuggag ugcuugugau ucugcucaug gugcaggaag ggcugaagaa gagaaugacc     3540

acaaagauca ucauaagcac aucaauggca gugcugguag cuaugauccu gggaggauuu     3600

ucaaugagug accuggcuaa gcuugcaauu uugaugggug ccaccuucgc ggaaaugaac     3660

acuggaggag auguagcuca ucuggcgcug auagcggcau ucaaagucag accagcguug     3720

cugguaucuu ucaucuucag agcuaauugg acaccccgug aaagcaugcu gcuggccuug     3780

gccucguguc uuuugcaaac ugcgaucucc gccuuggaag gcgaccugau gguucucauc     3840

aaugguuuug cuuuggccug guuggcaaua cgagcgaugg uuguuccacg cacugauaac     3900

aucaccuugg caauccuggc ugcucugaca ccacuggccc ggggcacacu gcuuguggcg     3960

uggagagcag gccuugcuac uugcgggggg uuuaugcucc ucucucugaa gggaaaaggc     4020

agugugaaga agaacuuacc auuugucaug gcccugggac uaaccgcugu gaggcugguc     4080

gaccccauca acgugguggg gcugcuguug cucacaagga gugggaagcg gagcuggccc     4140

ccuagcgaag uacucacagc uguuggccug auaugcgcau uggcuggagg guucgccaag     4200

gcagauauag agauggcugg gcccauggcc gcggucgguc ugcuaauugu caguuacgug     4260

gucucaggaa agagugugga cauguacauu gaaagagcag gugacaucac augggaaaaa     4320

gaugcggaag ucacuggaaa caguccccgg cucgaugugg cgcuagauga gaguggugau     4380

uucucccugg uggaggauga cggucccccc augagagaga ucauacucaa ggugguccug     4440

augaccaucu guggcaugaa cccaauagcc auacccuuug cagcuggagc gugguacgua     4500

uacgugaaga cuggaaaaag gaguggugcu cuaugggaug ugccugcucc caaggaagua     4560

aaaaaggggg agaccacaga uggaguguac agaguaauga cucguagacu gcuagguuca     4620

acacaaguug gagugggagu uaugcaagag ggggucuuuc acacuaugug gcacgucaca     4680

aaaggauccg cgcugagaag cggugaaggg agacuugauc cauacugggg agaugucaag     4740

caggaucugg ugucauacug ugguccaugg aagcuagaug ccgccuggga cgggcacagc     4800

gaggugcagc ucuuggccgu gccccccgga gagagagcga ggaacaucca gacucugccc     4860

ggaauauuua agacaaagga uggggacauu ggagcgguug cgcuggauua cccagcagga     4920

acuucaggau cuccaauccu agacaagugu gggagaguga uaggacuuua uggcaauggg     4980

gucgugauca aaaaugggag uuauguuagu gccaucaccc aagggaggag ggaggaagag     5040

acuccuguug agugcuucga gccuucgaug cugaagaaga agcagcuaac ugucuuagac     5100

uugcauccug gagcugggaa aaccaggaga guucuuccug aaauaguccg ugaagccaua     5160

aaaacaagac uccguacugu gaucuuagcu ccaaccaggg uugucgcugc ugaaauggag     5220

gaagcccuua gagggcuucc agugcguuau augacaacag cagucaaugu cacccacucu     5280

ggaacagaaa ucgucgacuu aaugugccau gccaccuuca cuucacgucu acuacagcca     5340

aucagagucc ccaacuauaa ucuguauauu auggaugagg cccacuucac agaucccuca     5400

aguauagcag caagaggaua cauuucaaca aggguugaga ugggcgaggc ggcugccauc     5460

uucaugaccg ccacgccacc aggaacccgu gacgcauuuc cggacuccaa cucaccaauu     5520

auggacaccg aaguggaagu cccagagaga gccuggagcu caggcuuuga uugggugacg     5580

gauuauucug gaaaaacagu uugguuuguu ccaagcguga ggaacggcaa ugagaucgca     5640

gcuugucuga caaaggcugg aaaacggguc auacagcuca gcagaaagac uuuugagaca     5700

gaguuccaga aaacaaaaca ucaagagugg gacuuugucg ugacaacuga cauuucagag     5760

augggcgcca acuuuaaagc ugaccguguc auagauucca ggagaugccu aaagccgguc     5820

auacuugaug gcgagagagu cauucuggcu ggacccaugc cugucacaca ugccagcgcu     5880

gcccagagga gggggcgcau aggcaggaau cccaacaaac cuggagauga guaucuguau     5940

ggaggugggu gcgcagagac ugacgaagac caugcacacu ggcuugaagc aagaaugcuc     6000

cuugacaaua uuuaccucca agauggccuc auagccucgc ucuaucgacc ugaggccgac     6060

aaaguagcag ccauugaggg agaguucaag cuuaggacgg agcaaaggaa gaccuuugug     6120

gaacucauga aaagaggaga ucuuccuguu uggcuggccu aucagguugc aucugccgga     6180

auaaccuaca cagauagaag auggugcuuu gauggcacga ccaacaacac cauaauggaa     6240

gacagugugc cggcagaggu guggaccaga cacggagaga aaagagugcu caaaccgagg     6300

uggauggacg ccagaguuug uucagaucau gcggcccuga agucauucaa ggaguuugcc     6360

gcugggaaaa gaggagcggc uuuuggagug auggaagccc ugggaacacu gccaggacac     6420

augacagaga gauuccagga agccauugac aaccucgcug ugcucaugcg ggcagagacu     6480

ggaagcaggc cuuacaaagc cgcggcggcc caauugccgg agacccuaga gaccauuaug     6540

cuuuuggggu ugcugggaac agucucgcug ggaaucuuuu ucgucuugau gaggaacaag     6600

ggcauaggga agaugggcuu uggaauggug acucuugggg ccagcgcaug gcucaugugg     6660

cucucggaaa uugagccagc cagaauugca uguguccuca uuguuguguu ccuauugcug     6720

guggugcuca uaccugagcc agaaaagcaa agaucucccc aggacaacca aauggcaauc     6780

aucaucaugg uagcaguagg ucuucugggc uugauuaccg ccaaugaacu cggaugguug     6840

gagagaacaa agagugaccu aagccaucua augggaagga gagaggaggg ggcaaccaug     6900

ggauucucaa uggacauuga ccugcggcca gccucagcuu gggccaucua ugcugccuug     6960

acaacuuuca uuaccccagc cguccaacau gcagugacca cuucauacaa caacuacucc     7020

uuaauggcga uggccacgca agcuggagug uuguuuggua ugggcaaagg gaugccauuc     7080

uacgcauggg acuuuggagu cccgcugcua augauagguu gcuacucaca auuaacgccc     7140

cugacccuaa uaguggccau cauuuugcuc guggcgcacu acauguacuu gaucccaggg     7200

cugcaggcag cagcugcgcg ugcugcccag aagagaacgg cagcuggcau caugaagaac     7260

ccuguugugg auggaauagu ggugacugac auugacacaa ugacaauuga cccccaagug     7320

gagaaaaaga ugggacaggu gcuacucaug gcaguagccg ucuccagcgc cauacugucg     7380

cggaccgccu gggggugggg ggaggcuggg gcccugauca cagccgcaac uuccacuuug     7440

ugggaaggcu cuccgaacaa guacuggaac uccucuacag ccacuucacu guguaacauu     7500

uuuaggggaa guuacuuggc uggagcuucu cuaaucuaca caguaacaag aaacgcuggc     7560

uuggucaaga gacguggggg uggaacagga gagacccugg gagagaaaug gaaggcccgc     7620

uugaaccaga ugucggcccu ggaguucuac uccuacaaaa agucaggcau caccgaggug     7680

ugcagagaag aggcccgccg cgcccucaag gacggugugg caacgggagg ccaugcugug     7740

ucccgaggaa gugcaaagcu gagaugguug guggagcggg gauaccugca gcccuaugga     7800

aaggucauug aucuuggaug uggcagaggg ggcuggaguu acuacgccgc caccauccgc     7860

aaaguucaag aagugaaagg auacacaaaa ggaggcccug gucaugaaga acccguguug     7920

gugcaaagcu auggguggaa cauaguccgu cuuaagagug ggguggacgu cuuucauaug     7980

gcggcugagc cgugugacac guugcugugu gacauaggug agucaucauc uaguccugaa     8040

guggaagaag cacggacgcu cagaguccuc uccauggugg gggauuggcu ugaaaaaaga     8100

ccaggagccu uuuguauaaa aguguugugc ccauacacca gcacuaugau ggaaacccug     8160

gagcgacugc agcguaggua ugggggagga cuggucagag ugccacucuc ccgcaacucu     8220

acacaugaga uguacugggu cucuggagcg aaaagcaaca ccauaaaaag uguguccacc     8280

acgagccagc uccucuuggg gcgcauggac gggccuagga ggccagugaa auaugaggag     8340

gaugugaauc ucggcucugg cacgcgggcu gugguaagcu gcgcugaagc ucccaacaug     8400

aagaucauug guaaccgcau ugaaaggauc cgcagugagc acgcggaaac gugguucuuu     8460

gacgagaacc acccauauag gacaugggcu uaccauggaa gcuaugaggc ccccacacaa     8520

gggucagcgu ccucucuaau aaacgggguu gucaggcucc ugucaaaacc cugggaugug     8580

gugacuggag ucacaggaau agccaugacc gacaccacac cguaugguca gcaaagaguu     8640

uucaaggaaa aaguggacac uagggugcca gacccccaag aagguacucg ucagguuaug     8700

agcauggucu cuuccugguu guggaaagag cuaggcaaac acaaacggcc acgagucugu     8760

accaaagaag aguucaucaa caagguucgu agcaaugcag cauuaggggc aauauuugaa     8820

gaggaaaaag aguggaagac ugcaguggaa gcugugaacg auccaagguu cugggcucua     8880

guggacaagg aaagagagca ccaccugaga ggagagugcc agaguugugu guacaacaug     8940

augggaaaaa gagaaaagaa acaaggggaa uuuggaaagg ccaagggcag ccgcgccauc     9000

ugguauaugu ggcuaggggc uagauuucua gaguucgaag cccuuggauu cuugaacgag     9060

gaucacugga uggggagaga gaacucagga ggugguguug aagggcuggg auuacaaaga     9120

cucggauaug uccuagaaga gaugagucgc auaccaggag gaaggaugua ugcagaugac     9180

acugcuggcu gggacacccg caucagcagg uuugaucugg agaaugaagc ucuaaucacc     9240

aaccaaaugg agaaagggca cagggccuug gcauuggcca uaaucaagua cacauaccaa     9300

aacaaagugg uaaagguccu uagaccagcu gaaaaaggga aaacaguuau ggacauuauu     9360

ucgagacaag accaaagggg gagcggacaa guugucacuu acgcucuuaa cacauuuacc     9420

aaccuagugg ugcaacucau ucggaauaug gaggcugagg aaguccuaga gaugcaagac     9480

uuguggcugc ugcggagguc agagaaagug accaacuggu ugcagagcaa cggaugggau     9540

aggcucaaac gaauggcagu caguggagau gauugcguug ugaagccaau ugaugauagg     9600

uuugcacaug cccucagguu cuugaaugau augggaaaag uuaggaagga cacacaagag     9660

uggaaacccu caacuggaug ggacaacugg gaagaaguuc cguuuugcuc ccaccacuuc     9720

aacaagcucc aucucaagga cgggaggucc auugugguuc ccugccgcca ccaagaugaa     9780

cugauuggcc gggcccgcgu cucuccaggg gcgggaugga gcauccggga gacugcuugc     9840

cuagcaaaau cauaugcgca aauguggcag cuccuuuauu uccacagaag ggaccuccga     9900

cugauggcca augccauuug uucaucugug ccaguugacu ggguuccaac ugggagaacu     9960

accuggucaa uccauggaaa gggagaaugg augaccacug aagacaugcu uguggugugg    10020

aacagagugu ggauugagga gaacgaccac auggaagaca agaccccagu uacgaaaugg    10080

acagacauuc ccuauuuggg aaaaagggaa gacuuguggu guggaucucu cauagggcac    10140

agaccgcgca ccaccugggc ugagaacauu aaaaacacag ucaacauggu gcgcaggauc    10200

auaggugaug aagaaaagua cauggacuac cuauccaccc aaguucgcua cuugggugaa    10260

gaagggucua caccuggagu gcuguaagga cuaguuauaa gacugacuag cccgaugggc    10320

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc    10440

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                10489


<210>  234
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 wild 
       type

<400>  234
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  235
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4803/R4833 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 1 wild type

<400>  235
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  236
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 wild
       type

<400>  236
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  237
<211>  10489
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein mRNA Design 1 wild 
       type

<400>  237
gggagaaagc uuaccaugaa aaacccaaaa aagaaauccg gaggauuccg gauugucaau       60

augcuaaaac gcggaguagc ccgugugagc cccuuugggg gcuugaagag gcugccagcc      120

ggacuucugc ugggucaugg gcccaucagg auggucuugg cgauucuagc cuuuuugaga      180

uucacggcaa ucaagccauc acugggucuc aucaauagau gggguucagu ggggaaaaaa      240

gaggcuaugg aaauaauaaa gaaguucaag aaagaucugg cugccaugcu gagaauaauc      300

aaugcuagga aggagaagaa gagacgaggc gcagauacua gugucggaau uguuggccuc      360

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      420

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      480

uguuauauac agaucaugga ucuuggacac acgugugaug ccaccaugag cuaugaaugc      540

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      600

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      660

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      720

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      780

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      840

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      900

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uguugucuug      960

gaacauggag guugugucac uguaauggca caggacaaac cgacugucga cauagagcug     1020

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua     1080

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa     1140

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu     1200

ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu uugcaugcuc caagaaaaug     1260

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1320

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1380

aaaguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg guuuggaagc     1440

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1500

augaauaaca agcacuggcu gguucacaag gagugguucc acgacauucc auuaccuugg     1560

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1620

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1680

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1740

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1800

uccuugugua cugcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1860

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1920

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1980

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     2040

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     2100

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     2160

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     2220

caucaaaucu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     2280

auucucauug gaacguugcu gaugugguug ggucugaacg caaagaaugg aucuauuucc     2340

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2400

guggggugcu cgguggacuu cucaaagaag gagacgagau gcgguacagg gguguucguc     2460

uauaacgacg uugaagccug gagggacagg uacaaguacc auccugacuc cccccguaga     2520

uuggcagcag cagucaagca agccugggaa gaugguaucu gcgggaucuc cucuguuuca     2580

agaauggaaa acaucaugug gagaucagua gaaggggagc ucaacgcaau ccuggaagag     2640

aauggaguuc aacugacggu cguuguggga ucuguaaaaa accccaugug gagaggucca     2700

cagagauugc ccgugccugu gaacgagcug ccccacggcu ggaaggcuug ggggaaaucg     2760

uacuucguca gagcagcaaa gacaaauaac agcuuugucg uggaugguga cacacugaag     2820

gaaugcccac ucaaacauag agcauggaac agcuuucuug uggaggauca uggguucggg     2880

guauuucaca cuagugucug gcucaagguu agagaagauu auucauuaga gugugaucca     2940

gccguuauug gaacagcugu uaagggaaag gaggcuguac acagugaucu aggcuacugg     3000

auugagagug agaagaauga cacauggagg cugaagaggg cccaucugau cgagaugaaa     3060

acaugugaau ggccaaaguc ccacacauug uggacagaug gaauagaaga gagugaucug     3120

aucauaccca agucuuuagc ugggccacuc agccaucaca auaccagaga gggcuacagg     3180

acccaaauga aagggccaug gcacagugaa gagcuugaaa uucgguuuga ggaaugccca     3240

ggcacuaagg uccacgugga ggaaacaugu ggaacgagag gaccaucucu gagaucaacc     3300

acugcaagcg gaagggugau cgaggaaugg ugcugcaggg agugcacaau gcccccacug     3360

ucguuccggg cuaaagaugg cuguugguau ggaauggaga uaaggcccag gaaagaacca     3420

gaaagcaacu uaguaagguc aauggugacu gcaggaucaa cugaucacau ggaccacuuc     3480

ucccuuggag ugcuugugau ucugcucaug gugcaggaag gguugaagaa gagaaugacc     3540

acaaagauca ucauaagcac aucaauggca gugcugguag cuaugauccu gggaggauuu     3600

ucaaugagug accuggcuaa gcuugcaauu uugaugggug ccaccuucgc ggaaaugaac     3660

acuggaggag auguagcuca ucuggcgcug auagcggcau ucaaagucag accagcguug     3720

cugguaucuu ucaucuucag agcuaauugg acaccccgug aaagcaugcu gcuggccuug     3780

gccucguguc uuuugcaaac ugcgaucucc gccuuggaag gcgaccugau gguucucauc     3840

aaugguuuug cuuuggccug guuggcaaua cgagcgaugg uuguuccacg cacugauaac     3900

aucaccuugg caauccuggc ugcucugaca ccacuggccc ggggcacacu gcuuguggcg     3960

uggagagcag gccuugcuac uugcgggggg uuuaugcucc ucucucugaa gggaaaaggc     4020

agugugaaga agaacuuacc auuugucaug gcccugggac uaaccgcugu gaggcugguc     4080

gaccccauca acgugguggg acugcuguug cucacaagga gugggaagcg gagcuggccc     4140

ccuagcgaag uacucacagc uguuggccug auaugcgcau uggcuggagg guucgccaag     4200

gcagauauag agauggcugg gcccauggcc gcggucgguc ugcuaauugu caguuacgug     4260

gucucaggaa agagugugga cauguacauu gaaagagcag gugacaucac augggaaaaa     4320

gaugcggaag ucacuggaaa caguccccgg cucgaugugg cgcuagauga gaguggugau     4380

uucucccugg uggaggauga cggucccccc augagagaga ucauacucaa ggugguccug     4440

augaccaucu guggcaugaa cccaauagcc auacccuuug cagcuggagc gugguacgua     4500

uacgugaaga cuggaaaaag gaguggugcu cuaugggaug ugccugcucc caaggaagua     4560

aaaaaggggg agaccacaga uggaguguac agaguaauga cucguagacu gcuagguuca     4620

acacaaguug gagugggagu uaugcaagag ggggucuuuc acacuaugug gcacgucaca     4680

aaaggauccg cgcugagaag cggugaaggg agacuugauc cauacugggg agaugucaag     4740

caggaucugg ugucauacug ugguccaugg aagcuagaug ccgccuggga cgggcacagc     4800

gaggugcagc ucuuggccgu gccccccgga gagagagcga ggaacaucca gacucugccc     4860

ggaauauuua agacaaagga uggggacauu ggagcgguug cgcuggauua cccagcagga     4920

acuucaggau cuccuauccu agacaagugu gggagaguga uaggacuuua uggcaauggg     4980

gucgugauca aaaaugggag uuauguuagu gccaucaccc aagggaggag ggaggaagag     5040

acuccuguug agugcuucga gccuucgaug cugaagaaga agcagcuaac ugucuuagac     5100

uugcauccug gagcugggaa aaccaggaga guucuuccug aaauaguccg ugaagccaua     5160

aaaacaagac uccguacugu gaucuuagcu ccaaccaggg uugucgcugc ugaaauggag     5220

gaggcccuua gagggcuucc agugcguuau augacaacag cagucaaugu cacccacucu     5280

ggaacagaaa ucgucgacuu aaugugccau gccaccuuca cuucgcgucu acuacagcca     5340

aucagagucc ccaacuauaa ucuguauauu auggaugagg cccacuucac agaucccuca     5400

aguauagcag caagaggaua cauuucaaca aggguugaga ugggcgaggc ggccgccauc     5460

uucaugaccg ccacgccacc aggaacccgu gacgcauuuc cggacuccaa cucaccaauu     5520

auggacaccg aaguggaagu cccagagaga gccuggagcu caggcuuuga uugggugacg     5580

gaucauucug gaaaaacagu uugguuuguu ccaagcguga ggaacggcaa ugagaucgca     5640

gcuugucuga caaaggcugg aaaacggguc auacagcuca gcagaaagac uuuugagaca     5700

gaguuccaga aaacaaaaca ucaagagugg gacuuugucg ugacaacuga cauuucagag     5760

augggcgcca acuuuaaagc ugaccguguc auagauucca ggagaugccu aaagccgguc     5820

auacuugaug gcgagagagu cauucuggcu ggacccaugc cugucacaca ugccagcgcu     5880

gcccagagga gggggcgcau aggcaggaau cccaacaaac cuggagauga guaucuguau     5940

ggaggugggu gcgcagagac ugacgaagac caugcacacu ggcuugaagc aagaaugcuc     6000

cuugacaaua uuuaccucca agauggccuc auagccucgc ucuaucgacc ugaggccgac     6060

aaaguagcag ccauugaggg agaguucaag cuuaggacgg agcaaaggaa gaccuuugug     6120

gaacucauga aaagaggaga ucuuccuguu uggcuggccu aucagguugc aucugccgga     6180

auaaccuaca cagauagaag auggugcuuu gauggcacga ccaacaacac cauaauggaa     6240

gacagugugc cggcagaagu guggaccaga cacggagaga aaagagugcu caaaccgagg     6300

uggauggacg ccagaguuug uucagaucau gcggcccuga agucauucaa ggaguuugcc     6360

gcugggaaaa gaggagcggc uuuuggagug auggaagccc ugggaacacu gccaggacac     6420

augacagaga gauuccagga agccauugac aaccucgcug ugcucaugcg ggcagagacu     6480

ggaagcaggc cuuacaaagc cgcggcggcc caauugccgg agacccuaga gaccauuaug     6540

cuuuuggggu ugcugggaac agucucgcug ggaaucuucu ucgucuugau gaggaacaag     6600

ggcauaggga agaugggcuu uggaauggug acucuugggg ccagcgcaug gcucaugugg     6660

cucucggaaa uugagccagc cagaauugca uguguccuca uuguuguguu ccuauugcug     6720

guggugcuca uaccugagcc agaaaagcaa agaucucccc aggacaacca aauggcaauc     6780

aucaucaugg uagcaguagg ucuucugggc uugauuaccg ccaaugaacu cggaugguug     6840

gagagaacaa agagugaccu aagccaucua augggaagga gagaggaggg ggcaaccaua     6900

ggauucucaa uggacauuga ccugcggcca gccucagcuu gggccaucua ugcugccuug     6960

acaacuuuca uuaccccagc cguccaacau gcagugacca ccucauacaa caacuacucc     7020

uuaauggcga uggccacgca agcuggagug uuguuuggua ugggcaaagg gaugccauuc     7080

uacgcauggg acuuuggagu cccgcugcua augauagguu gcuacucaca auuaacaccc     7140

cugacccuaa uaguggccau cauuuugcuc guggcgcacu acauguacuu gaucccaggg     7200

cugcaggcag cagcugcgcg ugcugcccag aagagaacgg cagcuggcau caugaagaac     7260

ccuguugugg auggaauagu ggugacugac auugacacaa ugacaauuga cccccaagug     7320

gagaaaaaga ugggacaggu gcuacucaua gcaguagccg ucuccagcgc cauacugucg     7380

cggaccgccu gggggugggg ggaggcuggg gcccugauca cagccgcaac uuccacuuug     7440

ugggaaggcu cuccgaacaa guacuggaac uccucuacag ccacuucacu guguaacauu     7500

uuuaggggaa guuacuuggc uggagcuucu cuaaucuaca caguaacaag aaacgcuggc     7560

uuggucaaga gacguggggg uggaacagga gagacccugg gagagaaaug gaaggcccgc     7620

uugaaccaga ugucggcccu ggaguucuac uccuacaaaa agucaggcau caccgaggug     7680

ugcagagaag aggcccgccg cgcccucaag gacggugugg caacgggagg ccaugcugug     7740

ucccgaggaa gugcaaagcu gagaugguug guggagcggg gauaccugca gcccuaugga     7800

aaggucauug aucuuggaug uggcagaggg ggcuggaguu acuacgccgc caccauccgc     7860

aaaguucaag aagugaaagg auacacaaaa ggaggcccug gucaugaaga acccguguug     7920

gugcaaagcu auggguggaa cauaguccgu cuuaagagug ggguggacgu cuuucauaug     7980

gcggcugagc cgugugacac guugcugugu gacauaggug agucaucauc uaguccugaa     8040

guggaagaag cacggacgcu cagaguccuc uccauggugg gggauuggcu ugaaaaaaga     8100

ccaggagccu uuuguauaaa aguguugugc ccauacacca gcacuaugau ggaaacccug     8160

gagcgacugc agcguaggua ugggggagga cuggucagag ugccacucuc ccgcaacucu     8220

acacaugaga uguacugggu cucuggagcg aaaagcaaca ccauaaaaag uguguccacc     8280

acgagccagc uccucuuggg gcgcauggac gggccuagga ggccagugaa auaugaggag     8340

gaugugaauc ucggcucugg cacgcgggcu gugguaagcu gcgcugaagc ucccaacaug     8400

aagaucauug guaaccgcau ugaaaggauc cgcagugagc acgcggaaac gugguucuuu     8460

gacgagaacc acccauauag gacaugggcu uaccauggaa gcuaugaggc ccccacacaa     8520

gggucagcgu ccucucuaau aaacgggguu gucaggcucc ugucaaaacc cugggaugug     8580

gugacuggag ucacaggaau agccaugacc gacaccacac cguaugguca gcaaagaguu     8640

uucaaggaaa aaguggacac uagggugcca gacccccaag aaggcacucg ucagguuaug     8700

agcauggucu cuuccugguu guggaaagag cuaggcaaac acaaacggcc acgagucugu     8760

accaaagaag aguucaucaa caagguucgu agcaaugcag cauuaggggc aauauuugaa     8820

gaggaaaaag aguggaagac ugcaguggaa gcugugaacg auccaagguu cugggcucua     8880

guggacaagg aaagagagca ccaccugaga ggagagugcc agaguugugu guacaacaug     8940

augggaaaaa gagaaaagaa acaaggggaa uuuggaaagg ccaagggcag ccgcgccauc     9000

ugguauaugu ggcuaggggc uagauuucua gaguucgaag cccuuggauu cuugaacgag     9060

gaucacugga uggggagaga gaacucagga ggugguguug aagggcuggg auuacaaaga     9120

cucggauaug uccuagaaga gaugagucgu auaccaggag gaaggaugua ugcagaugac     9180

acugcuggcu gggacacccg cauuagcagg uuugaucugg agaaugaagc ucuaaucacc     9240

aaccaaaugg agaaagggca cagggccuug gcauuggcca uaaucaagua cacauaccaa     9300

aacaaagugg uaaagguccu uagaccagcu gaaaaaggga aaacaguuau ggacauuauu     9360

ucgagacaag accaaagggg gagcggacaa guugucacuu acgcucuuaa cacauuuacc     9420

aaccuagugg ugcaacucau ucggaauaug gaggcugagg aaguucuaga gaugcaagac     9480

uuguggcugc ugcggagguc agagaaagug acuaacuggu ugcagagcaa cggaugggau     9540

aggcucaaac gaauggcagu caguggagau gauugcguug ugaagccaau ugaugauagg     9600

uuugcacaug cccucagguu cuugaaugau augggaaaag uuaggaagga cacacaagag     9660

uggaaacccu caacuggaug ggacaacugg gaagaaguuc cguuuugcuc ccaccacuuc     9720

aacaagcucc aucucaagga cgggaggucc auugugguuc ccugccgcca ccaagaugaa     9780

cugauuggcc gggcccgcgu cucuccaggg gcgggaugga gcauccggga gacugcuugc     9840

cuagcaaaau cauaugcgca aauguggcag cuccuuuauu uccacagaag ggaccuccga     9900

cugauggcca augccauuug uucaucugug ccaguugacu ggguuccaac ugggagaacu     9960

accuggucaa uccauggaaa gggagaaugg augaccacug aagacaugcu uguggugugg    10020

aacagagugu ggauugagga gaacgaccac auggaagaca agaccccagu uacgaaaugg    10080

acagacauuc ccuauuuggg aaaaagggaa gacuuguggu guggaucucu cauagggcac    10140

agaccgcgca ccaccugggc ugagaacauu aaaaacacag ucaacauggu gcgcaggauc    10200

auaggugaug aagaaaagua cauggacuac cuauccaccc aaguucgcua cuugggugaa    10260

gaagggucua caccuggagu gcuguaagga cuaguuauaa gacugacuag cccgaugggc    10320

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc    10440

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                10489


<210>  238
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 wild 
       type

<400>  238
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaaucuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  239
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4815/R4837 ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA 
       Design 1 wild type

<400>  239
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacgagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaaucuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  240
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 wild 
       type

<400>  240
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  241
<211>  10477
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein mRNA Design 1 wild type

<400>  241
gggagaaagc uuaccaugaa aaaccccaaa gaagaaaucc ggaggauccg gauugucaau       60

augcuaaaac gcggaguagc ccguguaaac cccuugggag guuugaagag guugccagcc      120

ggacuucugc ugggucaugg acccaucaga augguuuugg cgauacuagc cuuuuugaga      180

uuuacagcaa ucaagccauc acugggccuu aucaacagau gggguuccgu ggggaaaaaa      240

gaggcuaugg aaauaauaaa gaaguucaag aaagaucuug cugccauguu gagaauaauc      300

aaugcuagga aagagaggaa gagacguggc gcagacacca gcaucggaau cauuggccuc      360

cugcugacua cagccauggc agcagagauc acuagacgcg ggagugcaua cuacauguac      420

uuggauagga gcgaugccgg gaaggccauu ucguuugcua ccacauuggg agugaacaag      480

ugccacguac agaucaugga ccucgggcac augugugacg ccaccaugag uuaugagugc      540

ccuaugcugg augagggagu ggaaccagau gaugucgauu gcuggugcaa cacgacauca      600

acuuggguug uguacggaac cugucaucac aaaaaaggug aggcacggcg aucuagaaga      660

gccgugacgc ucccuucuca cucuacaagg aaguugcaaa cgcggucgca gaccugguua      720

gaaucaagag aauacacgaa gcacuugauc aagguugaaa acuggauauu caggaacccc      780

ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg gaagcucgac gagccaaaaa      840

gucauauacu uggucaugau acugcugauu gccccggcau acaguaucag gugcauugga      900

gucagcaaua gagacuucgu ggagggcaug ucagguggga ccuggguuga uguugucuug      960

gaacauggag gcugcguuac cgugauggca caggacaagc caacagucga cauagaguug     1020

gucacgacga cgguuaguaa cauggccgag guaagauccu auugcuacga ggcaucgaua     1080

ucggacaugg cuucggacag ucguugccca acacaaggug aagccuaccu ugacaagcaa     1140

ucagacacuc aauaugucug caaaagaaca uuaguggaca gagguugggg aaacgguugu     1200

ggacuuuuug gcaaagggag cuuggugaca ugugccaagu uuacguguuc uaagaagaug     1260

accgggaaga gcauucaacc ggaaaaucug gaguaucgga uaaugcuauc agugcauggc     1320

ucccagcaua gcgggaugau uggauaugaa acugacgaag auagagcgaa agucgagguu     1380

acgccuaauu caccaagagc ggaagcaacc uugggaggcu uuggaagcuu aggacuugac     1440

ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu accugaccau gaacaauaag     1500

cauugguugg ugcacaaaga gugguuucau gacaucccau ugccuuggca ugcuggggca     1560

gacaccggaa cuccacacug gaacaacaaa gaggcauugg uagaauucaa ggaugcccac     1620

gccaagaggc aaaccgucgu cguucugggg agccaggaag gagccguuca cacggcucuc     1680

gcuggagcuc uagaggcuga gauggauggu gcaaagggaa ggcuguucuc uggccauuug     1740

aaaugccgcc uaaaaaugga caagcuuaga uugaagggcg ugucauauuc cuugugcacu     1800

gcggcauuca cauucaccaa ggucccagcu gaaacacugc auggaacagu cacaguggag     1860

gugcaguaug cagggacaga uggacccugc aagaucccag uccagauggc gguggacaug     1920

cagacccuga ccccaguugg aaggcugaua accgccaacc ccgugauuac ugaaagcacu     1980

gagaacucaa agaugauguu ggagcuugac ccaccauuug gggauucuua cauugucaua     2040

ggaguugggg acaagaaaau cacccaccac uggcauagga gugguagcac caucggaaag     2100

gcauuugagg ccacugugag aggcgccaag agaauggcag uccuggggga uacagccugg     2160

gacuucggau cagucggggg uguguucaac ucacugggua agggcauuca ccagauuuuu     2220

ggagcagccu ucaaaucacu guuuggagga auguccuggu ucucacagau ccucauaggc     2280

acgcugcuag ugugguuagg uuugaacaca aagaauggau cuaucucccu cacaugcuug     2340

gcccuggggg gagugaugau cuuccucucc acggcuguuu cugcugacgu ggggugcuca     2400

guggacuucu caaaaaagga aacgagaugu ggcacggggg uauucaucua uaaugauguu     2460

gaagccugga gggaccggua caaguaccau ccugacuccc cccgcagauu ggcagcagca     2520

gucaagcagg ccugggaaga ggggaucugu gggaucucau ccguuucaag aauggaaaac     2580

aucaugugga aaucaguaga aggggagcuc aaugcuaucc uagaggagaa uggaguucaa     2640

cugacaguug uugugggauc uguaaaaaac cccaugugga gagguccaca aagauugcca     2700

gugccuguga augagcugcc ccauggcugg aaagccuggg ggaaaucgua uuuuguuagg     2760

gcggcaaaga ccaacaacag uuuuguuguc gacggugaca cacugaagga auguccgcuu     2820

gagcacagag cauggaauag uuuucuugug gaggaucacg gguuuggagu cuuccacacc     2880

agugucuggc uuaaggucag agaagauuac ucauuagaau gugacccagc cgucauagga     2940

acagcuguua agggaaggga ggccgcgcac agugaucugg gcuauuggau ugaaagugaa     3000

aagaaugaca cauggaggcu gaagagggcc caccugauug agaugaaaac augugaaugg     3060

ccaaagucuc acacauugug gacagaugga guagaagaaa gugaucuuau cauacccaag     3120

ucuuuagcug guccacucag ccaccacaac accagagagg guuacagaac ccaagugaaa     3180

gggccauggc acagugaaga gcuugaaauc cgguuugagg aauguccagg caccaagguu     3240

uacguggagg agacaugcgg aacuagagga ccaucucuga gaucaacuac ugcaagugga     3300

agggucauug aggaauggug cuguagggaa ugcacaaugc ccccacuauc guuucgagca     3360

aaagacggcu gcugguaugg aauggagaua aggcccagga aagaaccaga gagcaacuua     3420

gugaggucaa uggugacagc ggggucaacc gaucauaugg accacuucuc ucuuggagug     3480

cuugugauuc uacucauggu gcaggagggg uugaagaaga gaaugaccac aaagaucauc     3540

augagcacau caauggcagu gcugguaguc augaucuugg gaggauuuuc aaugagugac     3600

cuggccaagc uugugauccu gaugggugcu acuuucgcag aaaugaacac uggaggagau     3660

guagcucacu uggcauuggu agcggcauuu aaagucagac cagccuugcu ggucuccuuc     3720

auuuucagag ccaauuggac accccgugag agcaugcugc uagcccuggc uucgugucuu     3780

cugcaaacug cgaucucugc ucuugaaggu gacuugaugg uccucauuaa uggauuugcu     3840

uuggccuggu uggcaauucg agcaauggcc gugccacgca cugacaacau cgcucuacca     3900

aucuuggcug cucuaacacc acuagcucga ggcacacugc ucguggcaug gagagcgggc     3960

cuggcuacuu guggagggau caugcuccuc ucccugaaag ggaaagguag ugugaagaag     4020

aaccugccau uugucauggc ccugggauug acagcuguga ggguaguaga cccuauuaau     4080

gugguaggac uacuguuacu cacaaggagu gggaagcgga gcuggccccc uagugaaguu     4140

cucacagccg uuggccugau augugcacug gccggagggu uugccaaggc agacauugag     4200

auggcuggac ccauggcugc aguaggcuug cuaauuguca gcuauguggu cucgggaaag     4260

aguguggaca uguacauuga aagagcaggu gacaucacau gggaaaagga cgcggaaguc     4320

acuggaaaca guccucggcu ugacguggca cuggaugaga guggugacuu cuccuuggua     4380

gaggaagaug guccacccau gagagagauc auacucaagg ugguccugau ggccaucugu     4440

ggcaugaacc caauagcuau accuuuugcu gcaggagcgu gguaugugua ugugaagacu     4500

gggaaaagga guggcgcccu cugggacgug ccugcuccca aagaagugaa gaaaggagag     4560

accacagaug gaguguacag agugaugacu cgcagacugc uagguucaac acagguugga     4620

gugggaguca ugcaagaggg agucuuccac accauguggc acguuacaaa aggagccgca     4680

cugaggagcg gugagggaag acuugaucca uacugggggg augucaagca ggacuuggug     4740

ucauacugug ggccuuggaa guuggaugca gcuugggaug gacucagcga gguacagcuu     4800

uuggccguac cucccggaga gagggccaga aacauucaga cccugccugg aauauucaag     4860

acaaaggacg gggacaucgg agcaguugcu cuggacuacc cugcagggac cucaggaucu     4920

ccgauccuag acaaaugugg aagagugaua ggacucuaug gcaauggggu ugugaucaag     4980

aauggaagcu auguuagugc uauaacccag ggaaagaggg aggaggagac uccgguugaa     5040

uguuucgaac ccucgaugcu gaagaagaag cagcuaacug ucuuggaucu gcauccagga     5100

gccggaaaaa ccaggagagu ucuuccugaa auaguccgug aagccauaaa aaagagacuc     5160

cggacaguga ucuuggcacc aacuaggguu gucgcugcug agauggagga ggccuugaga     5220

ggacuuccgg ugcguuacau gacaacagca gucaacguca cccauucugg gacagaaauc     5280

guugauuuga ugugccaugc cacuuucacu ucacgcuuac uacaacccau cagagucccu     5340

aauuacaauc ucaacaucau ggaugaagcc cacuucacag accccucaag uauagcugca     5400

agaggauaca uaucaacaag gguugaaaug ggcgaggcgg cugccauuuu uaugacugcc     5460

acaccaccag gaacccguga ugcguuuccu gacucuaacu caccaaucau ggacacagaa     5520

guggaagucc cagagagagc cuggagcuca ggcuuugauu gggugacaga ccauucuggg     5580

aaaacaguuu gguucguucc aagcgugaga aacggaaaug aaaucgcagc cugucugaca     5640

aaggcuggaa agcgggucau acagcucagc aggaagacuu uugagacaga auuucagaaa     5700

acaaaaaauc aagaguggga cuuugucaua acaacugaca ucucagagau gggcgccaac     5760

uucaaggcug accgggucau agacucuagg agaugccuaa aaccagucau acuugauggu     5820

gagagaguca ucuuggcugg gcccaugccu gucacgcaug cuagugcugc ucagaggaga     5880

ggacguauag gcaggaaccc uaacaaaccu ggagaugagu acauguaugg aggugggugu     5940

gcagagacug augaaggcca ugcacacugg cuugaagcaa gaaugcuucu ugacaacauc     6000

uaccuccagg auggccucau agccucgcuc uaucggccug aggccgauaa gguagccgcc     6060

auugagggag aguuuaagcu gaggacagag caaaggaaga ccuucgugga acucaugaag     6120

agaggagacc uucccgucug gcuagccuau cagguugcau cugccggaau aacuuacaca     6180

gacagaagau ggugcuuuga uggcacaacc aacaacacca uaauggaaga caguguacca     6240

gcagagguuu ggacaaagua uggagagaag agagugcuca aaccgagaug gauggaugcu     6300

agggucuguu cagaccaugc ggcccugaag ucguucaaag aauucgccgc uggaaaaaga     6360

ggagcggcuu ugggaguaau ggaggcccug ggaacacugc caggacacau gacagagagg     6420

uuucaggaag ccauugacaa ccucgccgug cucaugcgag cagagacugg aagcaggccu     6480

uauaaggcag cggcagccca acugccggag acccuagaga ccauuaugcu cuuagguuug     6540

cugggaacag uuucacuggg gaucuucuuc gucuugaugc ggaauaaggg caucgggaag     6600

augggcuuug gaaugguaac ccuuggggcc agugcauggc ucauguggcu uucggaaauu     6660

gaaccagcca gaauugcaug uguccucauu guuguguuuu uauuacuggu ggugcucaua     6720

cccgagccag agaagcaaag aucuccccaa gauaaccaga uggcaauuau caucauggug     6780

gcagugggcc uucuagguuu gauaacugca aacgaacuug gauggcugga aagaacaaaa     6840

aaugacauag cucaucuaau gggaaggaga gaagaaggag caaccauggg auucucaaug     6900

gacauugauc ugcggccagc cuccgccugg gcuaucuaug ccgcauugac aacucucauc     6960

accccagcug uccaacaugc gguaaccacu ucauacaaca acuacuccuu aauggcgaug     7020

gccacacaag cuggagugcu guuuggcaug ggcaaaggga ugccauuuau gcauggggac     7080

cuuggagucc cgcugcuaau gauggguugc uauucacaau uaacaccccu gacucugaua     7140

guagcuauca uucugcuugu ggcgcacuac auguacuuga ucccaggccu acaagcggca     7200

gcagcgcgug cugcccagaa aaggacagca gcuggcauca ugaagaaucc cguuguggau     7260

ggaauagugg uaacugacau ugacacaaug acaauagacc cccaggugga gaagaagaug     7320

ggacaagugu uacucauagc aguagccauc uccagugcug ugcugcugcg gaccgccugg     7380

ggaugggggg aggcuggagc ucugaucaca gcagcgaccu ccaccuugug ggaaggcucu     7440

ccaaacaaau acuggaacuc cucuacagcc accucacugu gcaacaucuu cagaggaagc     7500

uaucuggcag gagcuucccu uaucuauaca gugacgagaa acgcuggccu gguuaagaga     7560

cguggaggug ggacgggaga gacucuggga gagaagugga aagcucgucu gaaucagaug     7620

ucggcccugg aguucuacuc uuauaaaaag ucagguauca cugaagugug uagagaggag     7680

gcucgccgug cccucaagga uggaguggcc acaggaggac augccguauc ccggggaagu     7740

gcaaagauca gaugguugga ggagagagga uaucugcagc ccuaugggaa gguuguugac     7800

cucggaugug gcagaggggg cuggagcuau uaugccgcca ccauccgcaa agugcaggag     7860

gugagaggau acacaaaggg aggucccggu caugaagaac ccaugcuggu gcaaagcuau     7920

ggguggaaca uaguucgucu caagagugga guggacgucu uccacauggc ggcugagccg     7980

ugugacacuc ugcuguguga cauaggugag ucaucaucua guccugaagu ggaagagaca     8040

cgaacacuca gagugcucuc uauggugggg gacuggcuug aaaaaagacc aggggccuuc     8100

uguauaaagg ugcugugccc auacaccagc acuaugaugg aaaccaugga gcgacugcaa     8160

cguaggcaug ggggaggauu agucagagug ccauuguguc gcaacuccac acaugagaug     8220

uacugggucu cuggggcaaa gagcaacauc auaaaaagug uguccaccac aagucagcuc     8280

cuccugggac gcauggaugg ccccaggagg ccagugaaau augaggagga ugugaaccuc     8340

ggcucgggua cacgagcugu ggcaagcugu gcugaggcuc cuaacaugaa aaucaucggc     8400

aggcgcauug agagaauccg caaugaacau gcagaaacau gguuucuuga ugaaaaccac     8460

ccauacagga caugggccua ccaugggagc uacgaagccc ccacgcaagg aucagcgucu     8520

ucccucguga acgggguugu uagacuccug ucaaagccuu gggacguggu gacuggaguu     8580

acaggaauag ccaugacuga caccacacca uacggccaac aaagagucuu caaagaaaaa     8640

guggacacca gggugccaga uccccaagaa ggcacucgcc agguaaugaa cauagucucu     8700

uccuggcugu ggaaggagcu ggggaaacgc aagcggccac gcgucugcac caaagaagag     8760

uuuaucaaca aggugcgcag caaugcagca cugggagcaa uauuugaaga ggaaaaagaa     8820

uggaagacgg cuguggaagc ugugaaugau ccaagguuuu gggcccuagu ggauagggag     8880

agagaacacc accugagagg agagugucac agcugugugu acaacaugau gggaaaaaga     8940

gaaaagaagc aaggagaguu cgggaaagca aaagguagcc gcgccaucug guacaugugg     9000

uugggagcca gauucuugga guuugaagcc cuuggauucu ugaacgagga ccauuggaug     9060

ggaagagaaa acucaggagg uggagucgaa ggguuaggau ugcaaagacu uggauacauu     9120

cuagaagaaa ugaaucgggc accaggagga aagauguacg cagaugacac ugcuggcugg     9180

gacacccgca uuaguaaguu ugaucuggag aaugaagcuc ugauuaccaa ccaaauggag     9240

gaagggcaca gaacucuggc guuggccgug auuaaauaca cauaccaaaa caaaguggug     9300

aagguucuca gaccagcuga aggaggaaaa acaguuaugg acaucauuuc aagacaagac     9360

cagagaggga guggacaagu ugucacuuau gcucucaaca cauucaccaa cuugguggug     9420

cagcuuaucc ggaacaugga agcugaggaa guguuagaga ugcaagacuu augguuguug     9480

aggaagccag agaaagugac cagaugguug cagagcaaug gaugggauag acucaaacga     9540

auggcgguca guggagauga cugcguugug aagccaaucg augauagguu ugcacaugcc     9600

cucagguucu ugaaugacau gggaaaaguu aggaaagaca cacaggagug gaaacccucg     9660

acuggaugga gcaauuggga agaagucccg uucugcuccc accacuucaa caagcuguac     9720

cucaaggaug ggagauccau uguggucccu ugccgccacc aagaugaacu gauuggccga     9780

gcucgcgucu caccaggggc aggauggagc auccgggaga cugccugucu ugcaaaauca     9840

uaugcgcaga uguggcagcu ccuuuauuuc cacagaagag accuucgacu gauggcuaau     9900

gccauuugcu cggcugugcc aguugacugg guaccaacug ggagaaccac cuggucaauc     9960

cauggaaagg gagaauggau gaccacugag gacaugcuca ugguguggaa uagagugugg    10020

auugaggaga acgaccauau ggaggacaag acuccuguaa caaaauggac agacauuccc    10080

uaucuaggaa aaagggagga cuuauggugu ggaucccuua uagggcacag accccgcacc    10140

acuugggcug aaaacaucaa agacacaguc aacauggugc gcaggaucau aggugaugaa    10200

gaaaaguaca uggacuaucu auccacccaa guccgcuacu ugggugagga aggguccaca    10260

cccggagugu uguaaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc    10320

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc    10440

ccccccccca aaggcucuuu ucagagccac cagaauu                             10477


<210>  242
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 wild type

<400>  242
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1080

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1140

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1200

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1260

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1320

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1380

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1440

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug     1500

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1560

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1620

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1680

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1740

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1800

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1860

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1920

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1980

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     2040

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2100

gcuguuucug cugacguggg gugcucagug gacuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  243
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4819/R4841 ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 
       1 wild type

<400>  243
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1080

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1140

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1200

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1260

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1320

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1380

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1440

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug     1500

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1560

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1620

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1680

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1740

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1800

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1860

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1920

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1980

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     2040

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2100

gcuguuucug cugacguggg gugcucagug gacuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  244
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 wild type

<400>  244
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      540

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      600

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      660

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      720

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag      780

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc      840

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca      900

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug      960

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1020

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1080

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1140

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1200

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1260

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1320

auggcagucc ugggggauac agccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  245
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 wild type

<400>  245
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      540

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      600

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      660

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      720

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag      780

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc      840

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca      900

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug      960

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1020

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1080

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1140

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1200

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1260

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1320

auggcagucc ugggggauac agccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  246
<211>  10489
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein mRNA Design 1 GC

<400>  246
gggagaaagc uuaccaugaa gaaccccaag aagaagagcg gcggguuccg caucgugaac       60

augcugaagc ggggcgucgc ccgcgugucc ccguucgggg gccucaagcg gcugcccgcc      120

gggcugcucc ugggccacgg ccccauccgc auggugcugg cgauccucgc cuuccugcgg      180

uucaccgcca ucaagcccag ccuggggcuc aucaaccgcu ggggcuccgu cgggaagaag      240

gaggccaugg agaucaucaa gaaguucaag aaggaccugg ccgcgaugcu gcggaucauc      300

aacgcccgca aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc      360

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcuccgccua cuacauguac      420

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      480

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      540

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      600

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      660

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      720

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      780

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      840

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      900

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      960

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc     1020

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc     1080

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag     1140

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc     1200

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug     1260

accgggaaga gcauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1320

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1380

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1440

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1500

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1560

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1620

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1680

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1740

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1800

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1860

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1920

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1980

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     2040

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     2100

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     2160

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     2220

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     2280

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2340

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2400

guggggugcu ccgucgacuu cagcaagaag gagacccgcu gcgggacggg cguguucgug     2460

uacaacgacg ucgaggccug gcgggaccgc uacaaguacc accccgacuc cccgcgccgg     2520

cuggccgccg cggugaagca ggccugggag gacgggaucu gcggcaucag cuccgugagc     2580

cgcauggaga acaucaugug gcggagcguc gagggggagc ugaacgccau ccucgaggag     2640

aacggcgugc agcugaccgu ggucgugggc uccgugaaga acccgaugug gcgcgggccc     2700

cagcggcugc ccgucccggu gaacgagcuc ccccacggcu ggaaggccug ggggaagagc     2760

cacuucgugc gcgcggccaa gaccaacaac uccuucgucg uggacggcga cacccugaag     2820

gagugccccc ugaagcaccg ggccuggaac agcuuccucg uggaggacca cggguucggc     2880

gucuuccaca cguccgugug gcugaaggug cgcgaggacu acagccugga gugcgacccc     2940

gccgucaucg gcaccgccgu gaaggggaag gaggcggugc acuccgaccu cggcuacugg     3000

aucgagagcg agaagaacga caccuggcgg cugaagcgcg cccaccugau cgagaugaag     3060

accugcgagu ggccgaaguc ccacacgcuc uggaccgacg ggaucgagga gagcgaccug     3120

aucaucccca agucccuggc cggcccccuc agccaccaca acacccggga gggguaccgc     3180

acccagauga agggccccug gcacuccgag gagcuggaga uccgguucga ggagugcccg     3240

ggcacgaagg uccacgugga ggagaccugc gggacccgcg gccccagccu gcgguccacc     3300

acggccagcg ggcgcgucau cgaggagugg ugcugccggg agugcaccau gcccccgcuc     3360

uccuuccgcg ccaaggacgg cugcugguac ggcauggaga uccggccgcg caaggagccc     3420

gagagcaacc uggugcgguc cauggugacc gcggggagca ccgaccacau ggaccacuuc     3480

agccugggcg uccucgugau ccugcugaug gugcaggagg ggcucaagaa gcgcaugacg     3540

accaagauca ucaucuccac cagcauggcc guccuggugg ccaugauccu gggcggguuc     3600

uccaugagcg accucgccaa gcuggccauc cugaugggcg cgaccuucgc cgagaugaac     3660

accggcgggg acguggccca ccucgcccug aucgccgcgu ucaagguccg ccccgcccug     3720

cucguguccu ucaucuuccg ggccaacugg acgccccgcg agagcaugcu gcuggcccuc     3780

gcguccugcc ugcugcagac cgccaucagc gcccucgagg gcgaccugau ggugcugauc     3840

aacggguucg cccucgccug gcuggcgauc cgggccaugg ucgugccgcg caccgacaac     3900

aucacccugg ccauccucgc cgcccugacg ccccuggcgc ggggcacccu ccugguggcc     3960

uggcgcgccg ggcuggccac gugcggcggc uucaugcucc ugucccugaa ggggaagggc     4020

agcgucaaga agaaccuccc cuucgugaug gcccuggggc ugaccgcggu gcggcucguc     4080

gaccccauca acgugguggg ccugcugcuc cugacgcgcu ccgggaagcg gagcuggccg     4140

cccuccgagg uccugaccgc cgugggccuc aucugcgccc uggccggcgg guucgcgaag     4200

gccgacaucg agauggccgg ccccauggcc gccguggggc ugcucaucgu cagcuacgug     4260

guguccggca agagcgucga cauguacauc gagcgcgcgg gggacaucac cugggagaag     4320

gacgccgagg ugaccggcaa cuccccgcgg cuggacgugg cccuggacga gagcggcgac     4380

uucucccucg ucgaggacga cgggccgccc augcgcgaga ucauccugaa gguggugcug     4440

augacgaucu gcggcaugaa ccccaucgcc aucccguucg ccgcgggggc cugguacguc     4500

uacgugaaga ccggcaagcg gagcggcgcc cugugggacg ugcccgcccc caaggagguc     4560

aagaaggggg agaccaccga cggcguguac cgcgugauga cgcggcgccu ccuggggagc     4620

acccaggucg gcgugggggu gaugcaggag ggcgucuucc acaccaugug gcacgugacc     4680

aagggcuccg cccugcggag cggggagggc cgccucgacc ccuacugggg cgacgugaag     4740

caggaccugg ucuccuacug cgggccgugg aagcuggacg cggccuggga cgggcacagc     4800

gaggugcagc uccuggccgu gcccccgggc gagcgggccc gcaacaucca gacgcugccc     4860

ggcaucuuca agaccaagga cggggacauc ggcgcggucg cccucgacua cccggccggg     4920

accuccggca gccccauccu ggacaagugc gggcgcguga ucggccugua cggcaacggg     4980

guggucauca agaacggcuc cuacgugagc gccaucaccc aggggcggcg cgaggaggag     5040

acgcccgucg agugcuucga gcccuccaug cucaagaaga agcagcugac cgugcuggac     5100

cuccacccgg gcgccgggaa gacccggcgc gugcugcccg agaucguccg ggaggcgauc     5160

aagacccgcc ugcggacggu gauccucgcc cccacccgcg uggucgccgc cgagauggag     5220

gaggcccugc ggggccugcc cgugcgcuac augaccaccg cggugaacgu cacgcacagc     5280

ggcaccgaga ucguggaccu caugugccac gccaccuuca ccucccggcu gcugcagccg     5340

auccgcgugc ccaacuacaa ccucuacauc auggacgagg cccacuucac ggaccccagc     5400

uccaucgccg cccgggggua caucagcacc cgcgucgaga ugggcgaggc ggccgccauc     5460

uucaugaccg ccaccccgcc ggggacccgg gacgccuucc ccgacuccaa cagccccauc     5520

auggacacgg agguggaggu gcccgagcgc gcguggucca gcggcuucga cugggucacc     5580

gacuacuccg gcaagaccgu gugguucgug ccgagcgucc ggaacgggaa cgagaucgcc     5640

gccugccuga ccaaggccgg caagcgcgug auccagcuga gccggaagac guucgagacc     5700

gaguuccaga agaccaagca ccaggagugg gacuucgugg ucaccacgga caucuccgag     5760

augggggcga acuucaaggc cgaccgcgug aucgacagcc ggcgcugccu caagcccgug     5820

auccuggacg gcgagcgcgu cauccuggcc gggcccaugc ccgugaccca cgccuccgcc     5880

gcgcagcggc gcggccggau cggccgcaac ccgaacaagc ccggggacga guaccucuac     5940

ggcgggggcu gcgccgagac cgacgaggac cacgcccacu ggcuggaggc ccggaugcug     6000

cucgacaaca ucuaccugca ggacgggcug aucgccagcc ucuaccgccc cgaggcggac     6060

aagguggccg ccaucgaggg cgaguucaag cugcggaccg agcagcgcaa gacguucguc     6120

gagcugauga agcggggcga ccucccggug uggcuggccu accagguggc cuccgcgggg     6180

aucaccuaca ccgaccgccg guggugcuuc gacggcacca cgaacaacac caucauggag     6240

gacagcgucc ccgccgaggu guggacccgc cacggggaga agcgggugcu gaagccccgc     6300

uggauggacg cccgggucug cuccgaccac gccgcgcuca agagcuucaa ggaguucgcc     6360

gccggcaagc gcggggccgc cuucggcgug auggaggcgc ugggcacccu gcccgggcac     6420

augacggagc gguuccagga ggccaucgac aaccucgccg ugcugaugcg cgccgagacc     6480

ggcucccggc cguacaaggc cgcggccgcc cagcugcccg agacccucga gaccaucaug     6540

cugcuggggc uccugggcac ggucagccug ggcaucuucu ucgugcucau gcgcaacaag     6600

gggaucggca agaugggguu cggcauggug acccuggggg ccuccgccug gcugaugugg     6660

cucagcgaga ucgagcccgc gcgcaucgcc ugcguccuga ucgugguguu ccugcuccug     6720

gucgugcuga uccccgagcc ggagaagcag cgguccccgc aggacaacca gauggccauc     6780

aucaucaugg uggccgucgg ccuccugggc cugaucaccg cgaacgagcu cggguggcug     6840

gagcgcacca agagcgaccu gucccaccuc augggccggc gcgaggaggg ggccacgaug     6900

ggcuucagca uggacaucga ccugcggccc gccuccgccu gggccaucua cgcggcccug     6960

accaccuuca ucacccccgc cgugcagcac gccgugacga ccagcuacaa caacuacucc     7020

cucauggcca uggcgaccca ggccgggguc cuguucggca ugggcaaggg gaugccguuc     7080

uacgccuggg acuucggcgu gccgcugcuc augaucgggu gcuacagcca gcugaccccg     7140

cugacgcuca ucguggccau cauccugcug gucgcccacu acauguaccu cauccccggc     7200

cugcaggcgg ccgccgcccg cgccgcgcag aagcggaccg ccgccgggau caugaagaac     7260

ccgguggucg acggcaucgu ggugaccgac aucgacacca ugacgaucga cccccagguc     7320

gagaagaaga ugggccaggu gcugcucaug gccguggcgg ucagcuccgc cauccugagc     7380

cgcaccgccu gggggugggg cgaggccggg gcccugauca ccgcggccac cuccacccuc     7440

ugggagggca gccccaacaa guacuggaac uccagcacgg ccaccucccu gugcaacauc     7500

uuccggggga gcuaccuggc cggcgccucc cucaucuaca ccgugacccg caacgcgggc     7560

cuggugaagc ggcgcggggg cgggacgggc gagacccugg gcgagaagug gaaggcccgg     7620

cucaaccaga ugagcgcccu ggaguucuac uccuacaaga agagcgggau caccgagguc     7680

ugccgcgagg aggcccggcg cgcccugaag gacggcgugg cgaccggggg ccacgccgug     7740

ucccggggga gcgccaagcu ccgcuggcug gucgagcggg gcuaccugca gcccuacggc     7800

aaggugaucg accuggggug cggccgcggg ggcugguccu acuacgccgc gacgauccgg     7860

aaggugcagg aggucaaggg guacaccaag ggcggcccgg ggcacgagga gcccgugcuc     7920

gugcagagcu acggcuggaa caucguccgc cugaaguccg ggguggacgu guuccacaug     7980

gccgccgagc ccugcgacac ccugcucugc gacaucggcg agagcuccag cagccccgag     8040

gucgaggagg cccgcacccu gcgggugcug uccauggugg gggacuggcu cgagaagcgc     8100

ccgggcgccu ucugcaucaa gguccugugc cccuacacga gcaccaugau ggagacccug     8160

gagcggcucc agcgccggua cggcgggggc cuggugcgcg ugccccuguc ccggaacagc     8220

acccacgaga uguacugggu cuccggggcg aagagcaaca cgaucaaguc cgugagcacc     8280

accucccagc uccugcuggg ccgcauggac ggcccccggc gcccggugaa guacgaggag     8340

gacgucaacc ucgggagcgg cacccgggcc gugguguccu gcgccgaggc ccccaacaug     8400

aagaucaucg ggaaccgcau cgagcggauc cgcagcgagc acgccgagac gugguucuuc     8460

gacgagaacc accccuaccg gaccugggcg uaccacggcu ccuacgaggc cccgacccag     8520

gggagcgccu ccagccugau caacggcguc gugcgccugc ucuccaagcc cugggacgug     8580

gucaccggcg ugacggggau cgccaugacc gacaccaccc ccuacggcca gcagcgggug     8640

uucaaggaga aggucgacac gcgcgugccc gacccgcagg aggggacccg ccaggugaug     8700

agcaugguca gcuccuggcu guggaaggag cugggcaagc acaagcgccc ccgcgugugc     8760

accaaggagg aguucaucaa caaggugcgg agcaacgccg cgcucggggc caucuucgag     8820

gaggagaagg aguggaagac cgccgucgag gccgugaacg acccccgcuu cugggcccug     8880

guggacaagg agcgggagca ccaccuccgc ggcgagugcc aguccugcgu cuacaacaug     8940

augggcaagc gggagaagaa gcagggggag uucggcaagg cgaaggggag ccgcgccauc     9000

ugguacaugu ggcucggcgc ccgguuccug gaguucgagg cccugggguu ccucaacgag     9060

gaccacugga ugggccgcga gaacuccggc gggggcgugg aggggcuggg ccugcagcgg     9120

cucggguacg ugcuggagga gaugagccgc auccccggcg gccggaugua cgcggacgac     9180

acggccgggu gggacacccg caucucccgg uucgaccugg agaacgaggc ccucaucacc     9240

aaccagaugg agaagggcca ccgcgcccug gcccuggcga ucaucaagua caccuaccag     9300

aacaaggucg ugaagguccu ccggccggcc gagaagggga agacggugau ggacaucauc     9360

agccgccagg accagcgggg cuccggccag guggucaccu acgcccugaa caccuucacc     9420

aaccuggugg ugcagcucau ccgcaacaug gaggccgagg agguccugga gaugcaggac     9480

cuguggcucc ugcggcgcag cgagaaggug accaacuggc ugcaguccaa cgggugggac     9540

cggcucaagc gcauggccgu gagcggcgac gacugcgucg ugaagcccau cgacgaccgc     9600

uucgcgcacg cccugcgguu ccugaacgac auggggaagg ugcgcaagga cacgcaggag     9660

uggaagcccu ccaccggcug ggacaacugg gaggaggucc ccuucugcag ccaccacuuc     9720

aacaagcucc accugaagga cgggcggucc aucguggugc cgugccgcca ccaggacgag     9780

cugaucggcc gggcccgcgu cagccccggc gccggguggu ccauccggga gaccgccugc     9840

cucgcgaaga gcuacgccca gauguggcag cugcuguacu uccaccgccg ggaccuccgc     9900

cugauggcca acgccaucug cuccagcgug cccguggacu ggguccccac cggccggacg     9960

accuggagca uccacgggaa gggcgagugg augaccaccg aggacaugcu gguggugugg    10020

aaccgcgucu ggaucgagga gaacgaccac auggaggaca agacgccggu gaccaagugg    10080

accgacaucc ccuaccucgg gaagcgggag gaccuguggu gcggcucccu gaucggccac    10140

cgcccccgga ccacgugggc ggagaacauc aagaacaccg ugaacauggu ccgccggauc    10200

aucggggacg aggagaagua cauggacuac cucagcaccc aggugcgcua ccugggcgag    10260

gaggggucca cccccggcgu gcugugagga cuaguuauaa gacugacuag cccgaugggc    10320

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc    10440

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                10489


<210>  247
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4804/R4834 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 1 GC

<400>  247
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  248
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 GC

<400>  248
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacau cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac cccgcacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcucgggu cccaggaggg cgccgugcac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcacgg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgccguucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  249
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4826/R4846 ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA 
       Design 1 GC

<400>  249
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      180

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      360

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug      840

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1080

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1140

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  250
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 GC

<400>  250
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacau cgugcuggag cacggcgggu gcgugaccgu cauggcgcag      180

gacaagccga cgguggacau cgagcucgug accaccaccg ucagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

gucgaccggg gcugggggaa cggcugcggg cucuucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ugggcggguu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg cgaagcgcca gaccguggug      840

guccugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg uccaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc ggugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  251
<211>  10489
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein mRNA Design 1 GC

<400>  251
gggagaaagc uuaccaugaa gaaccccaag aagaagagcg gcggguuccg caucgugaac       60

augcugaagc ggggcgucgc ccgcgugucc ccguucgggg gccucaagcg gcugcccgcc      120

gggcugcucc ugggccacgg ccccauccgc auggugcugg cgauccucgc cuuccugcgg      180

uucaccgcca ucaagcccag ccuggggcuc aucaaccgcu ggggcuccgu cgggaagaag      240

gaggccaugg agaucaucaa gaaguucaag aaggaccugg ccgcgaugcu gcggaucauc      300

aacgcccgca aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc      360

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcuccgccua cuacauguac      420

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      480

ugcuacaucc agaucaugga ccuggggcac acgugcgacg cgaccauguc cuacgagugc      540

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccacgagc      600

accugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      660

gccgugaccc ugcccagcca cuccacgcgc aagcugcaga cccggagcca gaccuggcuc      720

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      780

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac gagccagaag      840

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      900

gucagcaacc gggacuucgu ggaggggaug uccggcggca ccugggugga cgucgugcug      960

gagcacgggg gcugcgugac cgucauggcc caggacaagc ccaccgugga caucgagcuc     1020

gugacgacca ccgucagcaa cauggccgag gugcgcagcu acugcuacga ggccuccauc     1080

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag     1140

uccgacacgc aguacgucug caagcgcacc cugguggacc ggggcugggg gaacggcugc     1200

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug     1260

accgggaaga gcauccagcc ggagaaccug gaguaccgca ucaugcucuc cguccacggc     1320

agccagcacu ccgggaugau cgugaacgac acgggccacg agaccgacga gaaccgggcc     1380

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1440

cuggggcucg acugcgagcc ccggacgggc cuggacuuca gcgaccugua cuaccucacc     1500

augaacaaca agcacuggcu gguccacaag gagugguucc acgacauccc gcugcccugg     1560

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cguggaguuc     1620

aaggacgcgc acgccaagcg ccagacggug gucgugcugg gcucccagga gggggccgug     1680

cacaccgccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1740

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggucagcuac     1800

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacgcu ccacggcacc     1860

gugaccgugg agguccagua cgccgggacc gacggcccgu gcaaggugcc cgcccagaug     1920

gccguggaca ugcagacgcu gacccccguc gggcgccuga ucaccgcgaa ccccgugauc     1980

accgagagca cggagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     2040

uacaucguga ucggcgucgg ggagaagaag aucacccacc acuggcaccg gagcggcucc     2100

accaucggga aggccuucga ggccaccgug cgcggcgcca agcggauggc cgugcugggg     2160

gacacggcgu gggacuucgg cagcgucggc ggggcccuca acucccuggg caaggggauc     2220

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     2280

auccucaucg gcacccugcu gauguggcuc gggcugaacg ccaagaacgg cuccaucagc     2340

cugaugugcc ucgcgcuggg cggcgugcug aucuuccucu ccaccgccgu gagcgccgac     2400

gucgggugcu ccguggacuu cagcaagaag gagacccgcu gcgggacggg cguguucguc     2460

uacaacgacg uggaggccug gcgggaccgc uacaaguacc accccgacuc cccgcgccgg     2520

cuggccgcgg ccgugaagca ggccugggag gacgggaucu gcggcaucag cuccgucagc     2580

cgcauggaga acaucaugug gcggagcgug gagggggagc ugaacgccau ccucgaggag     2640

aacggcgugc agcugaccgu cguggugggc uccgucaaga acccgaugug gcgcgggccc     2700

cagcggcugc ccgugccggu gaacgagcuc ccccacggcu ggaaggcgug ggggaagagc     2760

uacuucgucc gcgccgccaa gaccaacaac uccuucgugg uggacggcga cacccugaag     2820

gagugccccc ugaagcaccg ggccuggaac agcuuccucg ucgaggacca cggguucggc     2880

guguuccaca cguccgugug gcugaagguc cgcgaggacu acagccugga gugcgacccc     2940

gccgugaucg gcaccgcggu gaaggggaag gaggccgucc acuccgaccu cggcuacugg     3000

aucgagagcg agaagaacga caccuggcgg cugaagcgcg cccaccugau cgagaugaag     3060

accugcgagu ggccgaaguc ccacacgcuc uggaccgacg ggaucgagga gagcgaccug     3120

aucaucccca agucccuggc cggcccccuc agccaccaca acacccggga gggguaccgc     3180

acccagauga agggccccug gcacuccgag gagcuggaga uccgguucga ggagugcccg     3240

ggcacgaagg ugcacgucga ggagaccugc gggacccgcg gccccagccu gcgguccacc     3300

acggccagcg ggcgcgugau cgaggagugg ugcugccggg agugcaccau gccgccccuc     3360

uccuuccgcg cgaaggacgg cugcugguac ggcauggaga uccggccgcg caaggagccc     3420

gagagcaacc uggugcgguc cauggucacc gccgggagca ccgaccacau ggaccacuuc     3480

agccugggcg ugcucgugau ccugcugaug guccaggagg ggcucaagaa gcgcaugacg     3540

accaagauca ucaucuccac cagcauggcc gugcuggugg ccaugauccu gggcggguuc     3600

uccaugagcg accucgccaa gcuggcgauc cugaugggcg ccaccuucgc cgagaugaac     3660

accggcgggg acgucgccca ccucgcccug aucgcggccu ucaaggugcg ccccgcccug     3720

cucguguccu ucaucuuccg ggccaacugg acgccccgcg agagcaugcu gcuggcgcuc     3780

gccuccugcc ugcugcagac cgccaucagc gcccucgagg gcgaccugau gguccugauc     3840

aacggguucg cccucgcgug gcuggccauc cgggccaugg uggugccgcg caccgacaac     3900

aucacccugg ccauccucgc cgcgcugacg ccccuggccc ggggcacccu ccuggucgcc     3960

uggcgcgccg ggcuggccac gugcggcggc uucaugcucc ugucccugaa ggggaagggc     4020

agcgugaaga agaaccuccc cuucgugaug gcgcuggggc ugaccgccgu ccggcucgug     4080

gaccccauca acguggucgg ccugcugcuc cugacgcgcu ccgggaagcg gagcuggccg     4140

cccuccgagg ugcugaccgc cgugggccuc aucugcgccc uggcgggcgg guucgccaag     4200

gccgacaucg agauggccgg ccccauggcc gcggucgggc ugcucaucgu gagcuacgug     4260

gucuccggca agagcgugga cauguacauc gagcgcgccg gggacaucac cugggagaag     4320

gacgccgagg ugaccggcaa cuccccgcgg cuggacgucg cccuggacga gagcggcgac     4380

uucucccucg uggaggacga cgggccgccc augcgcgaga ucauccugaa ggugguccug     4440

augacgaucu gcggcaugaa ccccaucgcc aucccguucg cggccggggc cugguacgug     4500

uacgugaaga ccggcaagcg gagcggcgcc cugugggacg uccccgcccc caaggaggug     4560

aagaaggggg agaccaccga cggcguguac cgcgucauga cgcggcgccu ccuggggagc     4620

acccaggugg gcgugggggu caugcaggag ggcguguucc acaccaugug gcacgugacc     4680

aagggcuccg cgcugcggag cggggagggc cgccucgacc ccuacugggg cgacgucaag     4740

caggaccugg uguccuacug cgggccgugg aagcuggacg ccgccuggga cgggcacagc     4800

gaggugcagc uccuggccgu cccgcccggc gagcgggcgc gcaacaucca gacgcugccc     4860

ggcaucuuca agaccaagga cggggacauc ggcgccgugg cccucgacua cccggccggg     4920

accuccggca gccccauccu ggacaagugc gggcgcguga ucggccugua cggcaacggg     4980

gucgugauca agaacggcuc cuacgucagc gccaucaccc aggggcggcg cgaggaggag     5040

acgcccgugg agugcuucga gcccuccaug cucaagaaga agcagcugac cgugcuggac     5100

cuccacccgg gcgcggggaa gacccggcgc guccugcccg agaucgugcg ggaggccauc     5160

aagacccgcc ugcggacggu gauccucgcc cccacccgcg ucguggccgc cgagauggag     5220

gaggcgcugc ggggccugcc cgugcgcuac augaccaccg ccgucaacgu gacgcacagc     5280

ggcaccgaga ucguggaccu caugugccac gccaccuuca ccucccggcu gcugcagccg     5340

auccgcgucc ccaacuacaa ccucuacauc auggacgagg cccacuucac ggaccccagc     5400

uccaucgccg cgcgggggua caucagcacc cgcguggaga ugggcgaggc cgccgccauc     5460

uucaugaccg ccaccccgcc ggggacccgg gacgcguucc ccgacuccaa cagccccauc     5520

auggacacgg agguggaggu ccccgagcgc gccuggucca gcggcuucga cugggugacc     5580

gaccacuccg gcaagaccgu gugguucguc ccgagcgugc ggaacgggaa cgagaucgcc     5640

gccugccuga ccaaggcggg caagcgcgug auccagcuga gccggaagac guucgagacc     5700

gaguuccaga agaccaagca ccaggagugg gacuucgucg ugaccacgga caucuccgag     5760

augggggcca acuucaaggc cgaccgcgug aucgacagcc ggcgcugccu caagcccguc     5820

auccuggacg gcgagcgcgu gauccuggcc gggcccaugc ccgugaccca cgccuccgcg     5880

gcccagcggc gcggccggau cggccgcaac ccgaacaagc ccggggacga guaccucuac     5940

ggcgggggcu gcgccgagac cgacgaggac cacgcccacu ggcuggaggc ccggaugcug     6000

cucgacaaca ucuaccugca ggacgggcug aucgcgagcc ucuaccgccc cgaggccgac     6060

aaggucgccg ccaucgaggg cgaguucaag cugcggaccg agcagcgcaa gacguucgug     6120

gagcugauga agcggggcga ccucccggug uggcuggccu accaggucgc guccgccggg     6180

aucaccuaca ccgaccgccg guggugcuuc gacggcacca cgaacaacac caucauggag     6240

gacagcgugc ccgccgaggu guggacccgc cacggggaga agcggguccu gaagccccgc     6300

uggauggacg cccgggugug cuccgaccac gcggcccuca agagcuucaa ggaguucgcc     6360

gccggcaagc gcggggccgc guucggcgug auggaggccc ugggcacccu gcccgggcac     6420

augacggagc gguuccagga ggccaucgac aaccucgccg uccugaugcg cgccgagacc     6480

ggcucccggc cguacaaggc ggccgccgcc cagcugcccg agacccucga gaccaucaug     6540

cugcuggggc uccugggcac ggugagccug ggcaucuucu ucgugcucau gcgcaacaag     6600

gggaucggca agaugggguu cggcaugguc acccuggggg ccuccgcgug gcugaugugg     6660

cucagcgaga ucgagcccgc ccgcaucgcc ugcgugcuga ucguggucuu ccugcuccug     6720

guggugcuga uccccgagcc ggagaagcag cgguccccgc aggacaacca gauggccauc     6780

aucaucaugg ucgcgguggg ccuccugggc cugaucaccg ccaacgagcu cggguggcug     6840

gagcgcacca agagcgaccu gucccaccuc augggccggc gcgaggaggg ggccacgauc     6900

ggcuucagca uggacaucga ccugcggccc gccuccgccu gggcgaucua cgccgcccug     6960

accaccuuca ucacccccgc cgugcagcac gccgucacga ccagcuacaa caacuacucc     7020

cucauggcga uggccaccca ggccggggug cuguucggca ugggcaaggg gaugccguuc     7080

uacgccuggg acuucggcgu gccccugcuc augaucgggu gcuacagcca gcugaccccg     7140

cugacgcuca ucgucgccau cauccugcug guggcgcacu acauguaccu cauccccggc     7200

cugcaggccg ccgccgcccg cgcggcccag aagcggaccg ccgccgggau caugaagaac     7260

ccggucgugg acggcaucgu ggucaccgac aucgacacca ugacgaucga cccccaggug     7320

gagaagaaga ugggccaggu gcugcucauc gcggucgccg ugagcuccgc cauccugagc     7380

cgcaccgccu gggggugggg cgaggccggg gcgcugauca ccgccgccac cuccacccuc     7440

ugggagggca gccccaacaa guacuggaac uccagcacgg ccaccucccu gugcaacauc     7500

uuccggggga gcuaccuggc cggcgcgucc cucaucuaca ccgugacccg caacgccggc     7560

cuggucaagc ggcgcggggg cgggacgggc gagacccugg gcgagaagug gaaggcccgg     7620

cucaaccaga ugagcgcccu ggaguucuac uccuacaaga agagcgggau caccgaggug     7680

ugccgcgagg aggcccggcg cgcgcugaag gacggcgugg ccaccggggg ccacgccguc     7740

ucccggggga gcgccaagcu ccgcuggcug guggagcggg gcuaccugca gcccuacggc     7800

aaggugaucg accuggggug cggccgcggg ggcugguccu acuacgcggc cacgauccgg     7860

aagguccagg aggugaaggg guacaccaag ggcggcccgg ggcacgagga gcccgugcuc     7920

guccagagcu acggcuggaa caucgugcgc cugaaguccg ggguggacgu cuuccacaug     7980

gccgccgagc ccugcgacac ccugcucugc gacaucggcg agagcuccag cagccccgag     8040

guggaggagg cccgcacccu gcgggugcug uccauggucg gggacuggcu cgagaagcgc     8100

ccgggcgcgu ucugcaucaa ggugcugugc cccuacacga gcaccaugau ggagacccug     8160

gagcggcucc agcgccggua cggcgggggc cuggugcgcg ucccccuguc ccggaacagc     8220

acccacgaga uguacugggu guccggggcc aagagcaaca cgaucaaguc cgugagcacc     8280

accucccagc uccugcuggg ccgcauggac ggcccccggc gcccggucaa guacgaggag     8340

gacgugaacc ucgggagcgg cacccgggcc guggucuccu gcgccgaggc ccccaacaug     8400

aagaucaucg ggaaccgcau cgagcggauc cgcagcgagc acgcggagac gugguucuuc     8460

gacgagaacc accccuaccg gaccugggcc uaccacggcu ccuacgaggc cccgacccag     8520

gggagcgccu ccagccugau caacggcgug gugcgccugc ucuccaagcc cugggacguc     8580

gugaccggcg ugacggggau cgccaugacc gacaccaccc ccuacggcca gcagcggguc     8640

uucaaggaga agguggacac gcgcgugccc gacccgcagg aggggacccg gcaggucaug     8700

agcaugguga gcuccuggcu guggaaggag cugggcaagc acaagcgccc ccgcgugugc     8760

accaaggagg aguucaucaa caagguccgg agcaacgcgg cccucggggc caucuucgag     8820

gaggagaagg aguggaagac cgccguggag gccgugaacg acccccgcuu cugggcgcug     8880

gucgacaagg agcgggagca ccaccuccgc ggcgagugcc aguccugcgu guacaacaug     8940

augggcaagc gggagaagaa gcagggggag uucggcaagg ccaaggggag ccgcgccauc     9000

ugguacaugu ggcucggcgc ccgguuccug gaguucgagg cgcugggguu ccucaacgag     9060

gaccacugga ugggccgcga gaacuccggc gggggcgugg aggggcuggg ccugcagcgg     9120

cucggguacg uccuggagga gaugagccgc auccccggcg gccggaugua cgccgacgac     9180

acggccgggu gggacacccg caucucccgg uucgaccugg agaacgaggc ccucaucacc     9240

aaccagaugg agaagggcca ccgcgcccug gcgcuggcca ucaucaagua caccuaccag     9300

aacaaggugg ucaaggugcu ccggccggcc gagaagggga agacggugau ggacaucauc     9360

agccgccagg accagcgggg cuccggccag gucgugaccu acgcccugaa caccuucacc     9420

aaccuggugg uccagcucau ccgcaacaug gaggccgagg aggugcugga gaugcaggac     9480

cuguggcucc ugcggcgcag cgagaaggug accaacuggc ugcaguccaa cgggugggac     9540

cggcucaagc gcauggcggu cagcggcgac gacugcgugg ugaagcccau cgacgaccgc     9600

uucgcccacg cccugcgguu ccugaacgac auggggaagg uccgcaagga cacgcaggag     9660

uggaagcccu ccaccggcug ggacaacugg gaggaggugc ccuucugcag ccaccacuuc     9720

aacaagcucc accugaagga cgggcggucc aucguggucc cgugccgcca ccaggacgag     9780

cugaucggcc gggcccgcgu gagccccggc gccggguggu ccauccggga gaccgcgugc     9840

cucgccaaga gcuacgccca gauguggcag cugcuguacu uccaccgccg ggaccuccgc     9900

cugauggcca acgcgaucug cuccagcgug cccgucgacu gggugcccac cggccggacg     9960

accuggagca uccacgggaa gggcgagugg augaccaccg aggacaugcu gguggucugg    10020

aaccgcgugu ggaucgagga gaacgaccac auggaggaca agacgccggu gaccaagugg    10080

accgacaucc ccuaccucgg gaagcgggag gaccuguggu gcggcucccu gaucggccac    10140

cgcccccgga ccacgugggc cgagaacauc aagaacaccg ucaacauggu gcgccggauc    10200

aucggggacg aggagaagua cauggacuac cucagcaccc aggugcgcua ccugggcgag    10260

gaggggucca cccccggcgu ccugugagga cuaguuauaa gacugacuag cccgaugggc    10320

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc    10440

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                10489


<210>  252
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4816/R4838 ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA 
       Design 1 GC

<400>  252
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacacg      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac cacgagcacc ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacgcgcaag      420

cugcagaccc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccacgag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg     1080

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc     1380

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cccugcugau guggcucggg     2040

cugaacgcca agaacggcuc caucagccug augugccucg cgcugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  253
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 GC

<400>  253
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacacg      240

ugcgacgcca ccauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagacgc gcagccagac cuggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgcgaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac acggcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgccguucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgugggcggc     1920

gcccugaacu cccucgggaa ggggauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacgcca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgucag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  254
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4825/R4845 ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA 
       Design 1 GC

<400>  254
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  255
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 GC

<400>  255
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccga cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cucuucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ugggcggguu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc ggugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  256
<211>  10477
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein mRNA Design 1 GC

<400>  256
gggagaaagc uuaccaugaa gaaccccaag gaggagaucc gccggauccg caucgugaac       60

augcugaagc ggggcgucgc ccgcgugaac ccgcucgggg gccugaagcg gcugcccgcc      120

gggcuccugc ugggccacgg gcccauccgc auggugcucg cgauccuggc cuuccugcgg      180

uucaccgcca ucaagcccag ccucggccug aucaaccgcu ggggcuccgu cgggaagaag      240

gaggccaugg agaucaucaa gaaguucaag aaggaccugg ccgcgaugcu ccggaucauc      300

aacgcccgca aggagcggaa gcgccggggc gccgacacca gcaucgggau caucggccug      360

cugcucacga ccgccauggc ggccgagauc acccgccggg gguccgccua cuacauguac      420

cuggaccgca gcgacgccgg caaggccauc uccuucgcga ccacgcuggg cgugaacaag      480

ugccacgugc agaucaugga ccucgggcac augugcgacg ccaccaugag cuacgagugc      540

ccgaugcugg acgagggcgu cgagcccgac gacguggacu gcuggugcaa caccaccucc      600

acgugggugg ucuacgggac cugccaccac aagaagggcg aggcccggcg cagccggcgc      660

gccgugaccc ugcccuccca cagcacccgg aagcuccaga cgcgcuccca gaccuggcug      720

gagagccgcg aguacaccaa gcaccugauc aagguggaga acuggaucuu ccggaacccc      780

ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg gguccagcac cucccagaag      840

gugaucuacc ucgucaugau ccugcugauc gccccggccu acagcauccg cugcaucggc      900

guguccaacc gggacuucgu ggaggggaug agcggcggga cgugggucga cguggugcuc      960

gagcacggcg gcugcgucac cgugauggcg caggacaagc ccaccgugga caucgagcug     1020

gucaccacga ccguguccaa cauggccgag guccgcagcu acugcuacga ggccagcauc     1080

uccgacaugg ccagcgacuc ccggugcccc acccaggggg aggccuaccu ggacaagcag     1140

agcgacaccc aguacgugug caagcgcacg cucguggacc ggggcugggg gaacggcugc     1200

gggcuguucg gcaagggcuc ccuggucacg ugcgcgaagu ucacgugcag caagaagaug     1260

accgggaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcugag cgugcacggc     1320

ucccagcaca gcgggaugau cggcuacgag acggacgagg accgggccaa gguggagguc     1380

accccgaacu ccccgcgcgc cgaggccacc cugggcgggu ucggcagccu cgggcuggac     1440

ugcgagcccc ggaccggccu ggacuucucc gaccucuacu accugacgau gaacaacaag     1500

cacuggcugg ugcacaagga gugguuccac gacauccccc ucccguggca cgcgggggcc     1560

gacaccggca ccccgcacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1620

gccaagcgcc agaccgucgu ggugcugggc agccaggagg gggcggucca cacggcccug     1680

gccggcgccc ucgaggccga gauggacggg gcgaagggcc ggcuguucuc cgggcaccug     1740

aagugccgcc ucaagaugga caagcugcgg cugaagggcg ugagcuacuc ccucugcacc     1800

gccgccuuca ccuucaccaa ggugcccgcc gagacccugc acggcacggu caccguggag     1860

gugcaguacg ccgggaccga cggccccugc aagaucccgg uccagauggc gguggacaug     1920

cagacccuga cgcccguggg gcgccucauc accgccaacc ccgucaucac cgagagcacc     1980

gagaacucca agaugaugcu ggagcuggac ccgccguucg gcgacagcua caucgugauc     2040

ggggugggcg acaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     2100

gccuucgagg ccaccguccg cggcgcgaag cggauggccg ugcucgggga caccgccugg     2160

gacuucggca gcgugggcgg cgucuucaac ucccugggga aggggaucca ccagaucuuc     2220

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggg     2280

acgcugcugg uguggcucgg ccugaacacc aagaacggcu ccaucagccu gaccugccuc     2340

gcgcugggcg gcgugaugau cuuccugucc accgccguca gcgccgacgu ggggugcucc     2400

guggacuuca gcaagaagga gacgcgcugc gggaccggcg ucuucaucua caacgacgug     2460

gaggccuggc gcgaccggua caaguaccac cccgacuccc cgcgccggcu cgccgcggcc     2520

gugaagcagg ccugggagga ggggaucugc ggcaucagcu ccgucagccg cauggagaac     2580

aucaugugga aguccgugga gggggagcug aacgccaucc uggaggagaa cggcgugcag     2640

cucaccgucg ugguggggag cgucaagaac cccauguggc ggggcccgca gcgccugccc     2700

gugcccguga acgagcugcc gcacggcugg aaggccuggg ggaagagcua cuucguccgg     2760

gcggccaaga ccaacaacuc cuucguggug gacggcgaca cgcucaagga gugcccccug     2820

gagcaccgcg ccuggaacag cuuccugguc gaggaccacg gguucggcgu guuccacacc     2880

uccguguggc ucaagguccg ggaggacuac agccuggagu gcgaccccgc cgugaucggg     2940

accgcgguca agggccgcga ggccgcccac uccgaccugg gcuacuggau cgagagcgag     3000

aagaacgaca ccuggcggcu caagcgcgcc caccugaucg agaugaagac gugcgagugg     3060

cccaaguccc acacccugug gaccgacggg guggaggaga gcgaccucau caucccgaag     3120

ucccuggccg gcccccugag ccaccacaac acccgggagg gguaccgcac ccaggugaag     3180

ggccccuggc acuccgagga gcucgagauc cgguucgagg agugccccgg gaccaagguc     3240

uacguggagg agaccugcgg cacccgcggc ccgagccugc gguccacgac cgcgagcggg     3300

cgcgugaucg aggaguggug cugccgggag ugcaccaugc cgccccuguc cuuccgcgcc     3360

aaggacggcu gcugguacgg gauggagauc cggccccgca aggagccgga gagcaaccuc     3420

guccgcucca uggugaccgc cggcagcacg gaccacaugg accacuucuc ccugggcgug     3480

cuggucaucc uccugauggu gcaggagggg cugaagaagc ggaugaccac caagaucauc     3540

augagcacca gcauggccgu gcucgucgug augauccugg gcggguucuc caugagcgac     3600

cuggccaagc ucgugauccu gaugggcgcg accuucgccg agaugaacac gggcggcgac     3660

gucgcccacc uggcccucgu ggccgcguuc aaggugcgcc ccgcccugcu ggucuccuuc     3720

aucuuccggg ccaacuggac cccgcgcgag agcaugcucc uggcccuggc cuccugccuc     3780

cugcagaccg cgaucagcgc ccuggagggg gaccucaugg ugcugaucaa cggguucgcc     3840

cuggccuggc ucgcgauccg ggccauggcc gugccccgca ccgacaacau cgcccugccg     3900

auccuggccg cgcucacgcc ccuggcccgg ggcacccugc ucgucgccug gcgcgccggg     3960

cuggccacgu gcggcgggau caugcugcuc ucccugaagg gcaagggcag cgugaagaag     4020

aaccugcccu ucgugauggc gcucgggcug accgccgucc ggguggugga ccccaucaac     4080

gucgugggcc ugcuccugcu gacgcgcucc gggaagcgga gcuggccgcc cuccgaggug     4140

cucaccgccg ucggccugau cugcgcccug gccgggggcu ucgcgaaggc cgacaucgag     4200

auggccggcc ccauggccgc gguggggcuc cugaucguga gcuacgucgu guccggcaag     4260

agcguggaca uguacaucga gcgcgccggg gacaucaccu gggagaagga cgccgagguc     4320

accggcaacu ccccgcggcu ggacguggcc cucgacgaga gcggggacuu cucccuggug     4380

gaggaggacg gcccgcccau gcgcgagauc auccugaagg ucgugcucau ggccaucugc     4440

ggcaugaacc ccaucgcgau cccguucgcc gccggggccu gguacgugua cgucaagacg     4500

ggcaagcgga gcggggcccu gugggacgug cccgcgccca aggaggugaa gaagggcgag     4560

accaccgacg gcgucuaccg cgugaugacc cggcgccugc ugggguccac ccaggugggc     4620

gucgggguga ugcaggaggg cguguuccac accauguggc acgucaccaa gggggccgcc     4680

cuccggagcg gcgagggccg ccuggacccc uacuggggcg acgugaagca ggaccugguc     4740

agcuacugcg ggccguggaa gcucgacgcc gccugggacg ggcuguccga ggugcagcug     4800

cucgcggugc cgcccggcga gcgcgcccgg aacauccaga cccugcccgg gaucuucaag     4860

acgaaggacg gcgacaucgg cgccgucgcc cuggacuacc cggcggggac cagcggcucc     4920

cccauccucg acaagugcgg gcgcgugauc ggccuguacg ggaacggcgu ggucaucaag     4980

aacggcagcu acguguccgc caucacccag gggaagcggg aggaggagac ccccguggag     5040

ugcuucgagc ccagcaugcu gaagaagaag cagcucacgg uccuggaccu gcacccgggc     5100

gccgggaaga cccgccgggu gcuccccgag aucgugcgcg aggccaucaa gaagcggcug     5160

cgcaccguca uccuggcccc cacccgggug guggcggccg agauggagga ggcccuccgc     5220

ggccugcccg uccgguacau gacgaccgcc gugaacguga cccacuccgg gaccgagauc     5280

gucgaccuga ugugccacgc cacguucacc agccgccucc ugcagccgau ccgggugccc     5340

aacuacaacc ugaacaucau ggacgaggcg cacuucaccg accccuccag caucgccgcc     5400

cgcggcuaca ucuccacccg gguggagaug ggcgaggccg ccgcgaucuu caugacggcc     5460

accccgccgg ggacccgcga cgccuucccc gacagcaacu cccccaucau ggacaccgag     5520

gucgaggugc ccgagcgggc cuggagcucc ggcuucgacu gggugaccga ccacagcggg     5580

aagacggucu gguucgugcc guccgugcgc aacggcaacg agaucgccgc gugccucacc     5640

aaggccggca agcgggucau ccagcugagc cgcaagaccu ucgagaccga guuccagaag     5700

acgaagaacc aggaguggga cuucgugauc accaccgaca ucuccgagau gggggccaac     5760

uucaaggccg accgggugau cgacagccgc cgcugccuga agcccgucau ccucgacggc     5820

gagcggguga uccuggcggg gcccaugccc gugacccacg ccagcgccgc ccagcgccgg     5880

ggccgcaucg ggcggaaccc gaacaagccc ggcgacgagu acauguacgg cgggggcugc     5940

gccgagacgg acgaggggca cgcgcacugg cuggaggccc gcaugcuccu ggacaacauc     6000

uaccugcagg acggccucau cgccucccug uaccggcccg aggccgacaa ggucgccgcg     6060

aucgaggggg aguucaagcu gcgcaccgag cagcggaaga ccuucgugga gcucaugaag     6120

cgcggcgacc ugccggugug gcuggccuac caggucgcca gcgccggcau caccuacacg     6180

gaccggcgcu ggugcuucga cgggaccacc aacaacacca ucauggagga cuccgugccc     6240

gccgaggugu ggacgaagua cggcgagaag cggguccuca agccccgcug gauggacgcg     6300

cgggugugca gcgaccacgc cgcccugaag uccuucaagg aguucgccgc ggggaagcgc     6360

ggcgccgccc ugggggugau ggaggcccuc ggcacccugc ccggccacau gaccgagcgg     6420

uuccaggagg ccaucgacaa ccuggcgguc cucaugcgcg ccgagaccgg gagccggccg     6480

uacaaggccg ccgccgcgca gcugcccgag acgcuggaga ccaucaugcu ccugggccug     6540

cucgggaccg ugucccuggg caucuucuuc gugcugaugc gcaacaaggg caucgggaag     6600

augggcuucg ggauggucac ccucggcgcc agcgccuggc ugauguggcu guccgagauc     6660

gagcccgccc gcaucgccug cgugcucauc guggucuucc ugcugcucgu ggugcugauc     6720

cccgagccgg agaagcagcg gagcccccag gacaaccaga uggcgaucau caucaugguc     6780

gccguggggc ugcucggccu gaucacggcc aacgagcugg gcuggcucga gcgcaccaag     6840

aacgacaucg cccaccugau ggggcggcgc gaggagggcg cgaccauggg guucuccaug     6900

gacaucgacc ugcggcccgc cagcgccugg gccaucuacg ccgcgcucac cacgcugauc     6960

acccccgccg uccagcacgc cgugaccacc uccuacaaca acuacagccu gauggccaug     7020

gccacgcagg cgggcgugcu cuucgggaug ggcaagggca ugccguucau gcacggggac     7080

cugggcgucc cgcugcucau gauggggugc uacucccagc ugaccccgcu gacccucauc     7140

guggccauca uccugcuggu ggcccacuac auguaccuca uccccggccu gcaggccgcc     7200

gcggcccgcg ccgcccagaa gcggaccgcc gcggggauca ugaagaaccc ggucguggac     7260

ggcaucgugg ucacggacau cgacaccaug accaucgacc cccaggugga gaagaagaug     7320

ggccaggugc ugcucaucgc cgucgccauc agcuccgccg ugcugcugcg caccgcgugg     7380

ggguggggcg aggccggggc ccucaucacg gccgccacca gcacccugug ggagggcucc     7440

cccaacaagu acuggaacag cagcaccgcg accucccugu gcaacaucuu ccgggggagc     7500

uaccucgccg gcgccucccu gaucuacacg gugacccgca acgccggccu ggucaagcgg     7560

cgcgggggcg ggaccggcga gacccucggc gagaagugga aggcccggcu gaaccagaug     7620

agcgcgcugg aguucuacuc cuacaagaag agcgggauca cggaggugug ccgcgaggag     7680

gcccggcgcg cccucaagga cggcguggcc accgggggcc acgccgucuc ccgggggagc     7740

gcgaagaucc gcuggcugga ggagcggggc uaccugcagc ccuacggcaa ggugguggac     7800

cuggggugcg gccgcggggg cugguccuac uacgccgcca ccauccggaa gguccaggag     7860

gugcgcgggu acaccaaggg cggcccgggg cacgaggagc ccaugcucgu gcagagcuac     7920

ggcuggaaca ucguccgccu gaaguccggg guggacgugu uccacauggc cgcggagccc     7980

ugcgacacgc ugcucugcga caucggcgag agcuccagcu cccccgaggu cgaggagacc     8040

cggacccugc gcgugcugag cauggugggg gacuggcucg agaagcggcc gggcgccuuc     8100

ugcaucaagg uccugugccc cuacaccucc acgaugaugg agaccaugga gcgccugcag     8160

cggcgccacg gcgggggccu cgugcgggug ccccugugcc gcaacagcac ccacgagaug     8220

uacugggucu ccggggccaa gagcaacauc aucaagagcg uguccaccac gagccagcug     8280

cuccugggcc ggauggacgg cccccgccgg ccggugaagu acgaggagga cgucaaccug     8340

ggguccggca cccgcgccgu ggccagcugc gcggaggccc ccaacaugaa gaucaucggg     8400

cggcgcaucg agcggauccg caacgagcac gccgagaccu gguuccucga cgagaaccac     8460

cccuaccgga ccugggccua ccacggcucc uacgaggccc cgacgcaggg gagcgcgucc     8520

agccugguga acggcgucgu gcgccugcuc uccaagcccu gggacguggu caccggcgug     8580

accgggaucg ccaugaccga cacgaccccc uacggccagc agcggguguu caaggagaag     8640

gucgacaccc gcgugcccga cccgcaggag gggacccgcc aggugaugaa caucgucagc     8700

uccuggcugu ggaaggagcu gggcaagcgg aagcgccccc gggugugcac gaaggaggag     8760

uucaucaaca aggugcgcag caacgccgcc cucggggcca ucuucgagga ggagaaggag     8820

uggaagaccg cggucgaggc cgugaacgac ccccgguucu gggcccuggu cgaccgcgag     8880

cgggagcacc accuccgcgg cgagugccac uccugcgugu acaacaugau gggcaagcgg     8940

gagaagaagc agggggaguu cggcaaggcc aaggggagcc gcgccaucug guacaugugg     9000

cucggcgcgc gguuccugga guucgaggcc cugggguucc ucaacgagga ccacuggaug     9060

ggccgcgaga acuccggcgg gggcguggag gggcugggcc ugcagcggcu cggguacauc     9120

cuggaggaga ugaaccgcgc ccccggcggc aagauguacg ccgacgacac cgcggggugg     9180

gacacccgga ucagcaaguu cgaccuggag aacgaggccc ucaucacgaa ccagauggag     9240

gagggccacc gcacccuggc ccuggccguc aucaaguaca ccuaccagaa caagguggug     9300

aagguccucc ggccggccga gggcgggaag accgugaugg acaucaucuc ccgccaggac     9360

cagcggggca gcggccaggu ggucacguac gcgcugaaca ccuucaccaa ccugguggug     9420

cagcucaucc gcaacaugga ggccgaggag guccuggaga ugcaggaccu guggcuccug     9480

cggaagcccg agaaggugac ccgcuggcug cagagcaacg gcugggaccg ccucaagcgg     9540

auggccgugu ccggggacga cugcgucgug aagcccaucg acgaccgcuu cgcccacgcc     9600

cugcgguucc ugaacgacau gggcaaggug cgcaaggaca cccaggagug gaagcccagc     9660

acgggguggu ccaacuggga ggaggucccg uucugcagcc accacuucaa caagcucuac     9720

cugaaggacg gccgguccau cguggugccc ugccgccacc aggacgagcu gaucggccgg     9780

gcgcgcguca gccccggggc cggcuggucc auccgggaga ccgccugccu cgccaagagc     9840

uacgcccaga uguggcagcu gcuguacuuc caccgccggg accuccgccu gauggcgaac     9900

gccaucugcu ccgccgugcc cguggacugg gucccgaccg ggcggaccac guggagcauc     9960

cacggcaagg gggaguggau gaccaccgag gacaugcuga ugguguggaa ccgcgugugg    10020

aucgaggaga acgaccacau ggaggacaag acccccguca cgaaguggac cgacaucccc    10080

uaccucggca agcgggagga ccuguggugc ggcucccuga ucgggcaccg cccccggacc    10140

accugggccg agaacaucaa ggacacggug aacauggugc gccggaucau cggcgacgag    10200

gagaaguaca uggacuaccu cagcacccag guccgcuacc ugggggagga gggcuccacc    10260

ccgggggugc ugugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc    10320

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc    10440

ccccccccca aaggcucuuu ucagagccac cagaauu                             10477


<210>  257
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4820/R4842 ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 
       1 GC

<400>  257
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agcggaagcg ccggggcgcc       60

gacaccagca ucgggaucau cggccugcug cucacgaccg ccauggcggc cgagaucacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      180

uucgcgacca cgcugggcgu gaacaagugc cacgugcaga ucauggaccu cgggcacaug      240

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccugc ccucccacag cacccggaag      420

cuccagacgc gcucccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      480

guggagaacu ggaucuuccg gaaccccggc uucgcccucg ucgcgguggc caucgccugg      540

cugcuggggu ccagcaccuc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc      600

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      660

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      840

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      900

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      960

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg     1080

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug     1140

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac     1200

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc     1380

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg     1440

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug     1500

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1620

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1680

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1740

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1860

auggccgugc ucggggacac cgccugggac uucggcagcg ugggcggcgu cuucaacucc     1920

cuggggaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucgggacg cugcuggugu ggcucggccu gaacaccaag     2040

aacggcucca ucagccugac cugccucgcg cugggcggcg ugaugaucuu ccuguccacc     2100

gccgucagcg ccgacguggg gugcuccgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  258
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 GC

<400>  258
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gccggacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacc     1080

gacgaggacc gcgccaaggu cgaggugacg cccaacuccc cgcgggccga ggcgacccuc     1140

gggggcuucg ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggggccgac acgggcaccc cgcacuggaa caacaaggag     1320

gcccuggucg aguucaagga cgcccacgcg aagcggcaga ccgugguggu ccucgggagc     1380

caggagggcg ccgugcacac cgcccuggcc ggggcgcugg aggccgagau ggacggcgcc     1440

aagggccgcc ucuucuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug     1500

aagggcguga gcuacucccu gugcacggcc gccuucaccu ucaccaaggu ccccgcggag     1560

acccuccacg ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc cggucgggcg gcugaucacg     1680

gccaacccgg ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg     1740

cccuucggcg acagcuacau cgugaucggg gucggcgaca agaagaucac ccaccacugg     1800

caccgcuccg ggagcacgau cggcaaggcc uucgaggcga ccgugcgggg cgccaagcgc     1860

auggccgugc ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc     1920

cuggggaagg gcauccacca gaucuucggc gccgcguuca agagccucuu cgggggcaug     1980

uccugguuca gccagauccu gaucgggacc cugcucgugu ggcugggccu gaacacgaag     2040

aacgggucca ucagccucac cugccuggcc cugggcggcg ucaugaucuu ccucuccacc     2100

gccgugagcg ccgacguggg gugcuccguc gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  259
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 GC

<400>  259
gggagaaagc uuaccauguc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc       60

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      120

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      180

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      300

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      420

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg      540

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug      600

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac      660

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc      840

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg      900

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug      960

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1080

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1140

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1200

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1260

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1320

auggccgugc ucggggacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  260
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 GC

<400>  260
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccga cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cucuucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgg cuacgagacc      540

gacgaggacc gggccaaggu ggaggugacc cccaacuccc cgcgcgccga ggccacgcug      600

ggcggguucg gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac      660

cuguacuacc ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc gggggccgac accggcacgc cgcacuggaa caacaaggag      780

gcccucgugg aguucaagga cgcccacgcc aagcgccaga ccguggucgu gcuggggagc      840

caggagggcg cggugcacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg      900

aagggccggc uguucagcgg gcaccugaag ugccgccuca agauggacaa gcugcggcug      960

aagggcgucu ccuacagccu cugcaccgcc gccuucacgu ucaccaaggu gcccgccgag     1020

acccugcacg ggaccgucac gguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggcggu ggacaugcag acccugaccc cgguggggcg ccucaucacg     1140

gccaacccgg ucaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg     1200

cccuucggcg acuccuacau cgugaucggg gugggcgaca agaagaucac ccaccacugg     1260

caccggagcg gguccacgau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1320

auggcggugc ucggggacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  261
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4805/R4835 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 1 optimized

<400>  261
gggagaaagc uuaccaugcg gaucaucaac gcccggaaag agaagaagcg cagaggcgcc       60

gauaccagcg ugggcauugu gggccugcug cugacaacag ccauggccgc cgaagugacc      120

agaagaggca gcgccuacua cauguaccug gaccggaacg augccggcga ggccaucagc      180

uuuccaacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga acccgacgau      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ucaccacaag      360

aagggcgaag ccagacgguc cagacgggcc gugacacugc cuagccacag caccagaaag      420

cugcagaccc ggucccagac cuggcuggaa agcagagagu acaccaagca ccugauccgg      480

guggaaaacu ggaucuuccg gaaccccggc uuugcccugg ccgcugcugc uauugcuugg      540

cugcugggca gcagcaccuc ccagaaagug aucuaccucg ugaugauccu gcugaucgcc      600

ccugccuaca gcauccggug uaucggcgug uccaaccggg acuucgugga aggcaugagc      660

ggcggcacau ggguggacau cgugcuggaa cacggcggcu gcgugacagu gauggcccag      720

gauaagccca ccguggauau cgagcucgug accaccaccg uguccaauau ggccgaagug      780

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augcccuaca      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgugugcaa gcggacccug      900

guggauagag gcuggggcaa uggcugcggc cuguuuggca agggcagccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa     1020

uaccggauca ugcugagcgu gcacggcagc cagcacuccg gcaugaucgu gaacgacacc     1080

ggccacgaga cagacgagaa ccgggccaag guggaaauca cccccaacag cccuagagcc     1140

gaggccacac ugggcggcuu uggaucucug ggccuggacu gcgagccuag aaccggccug     1200

gauuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaagag     1260

ugguuccacg acaucccccu gcccuggcau gccggcgcug auacaggcac accccacugg     1320

aacaacaaag aggcucuggu ggaguucaag gacgcccacg ccaagaggca gaccguggug     1380

gugcugggau cucaggaagg cgccgugcau acagcucugg cuggcgcccu ggaagccgaa     1440

auggauggcg cuaagggcag acuguccagc ggccaccuga agugccggcu gaagauggac     1500

aagcugcggc ugaagggcgu guccuacagc cuguguaccg ccgccuucac cuucaccaag     1560

auccccgccg agacacugca cggcaccgug acuguggaag ugcaguacgc cggcaccgac     1620

ggcccuugua aagugccugc ucagauggcu guggacaugc agacccugac cccugugggc     1680

aggcugauca ccgccaaccc ugugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gaacuggacc cacccuucgg cgacagcuac aucgugaucg gcguggggga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuuugaggc uacagugcgg     1860

ggagccaaga gaauggccgu gcugggagau accgccuggg acuuuggcuc ugugggcgga     1920

gcccugaacu cucugggcaa gggaauccac cagaucuucg gagccgccuu uaagagccug     1980

uucggcggca uguccugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucucccug augugccugg cucugggagg cgugcugauc     2100

uuccugagca cagccguguc ugccgacgug ggcugcagcg uggacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  262
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 
       optimized

<400>  262
gggagaaagc uuaccauguc ccagaaagug aucuaccucg ugaugauccu gcugaucgcc       60

ccugccuaca gcauccggug uaucggcgug uccaaccggg acuucgugga aggcaugagc      120

ggcggcacau ggguggacau cgugcuggaa cacggcggcu gcgugacagu gauggcccag      180

gauaagccca ccguggauau cgagcucgug accaccaccg uguccaauau ggccgaagug      240

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augcccuaca      300

cagggcgagg ccuaccugga caagcagucc gacacccagu acgugugcaa gcggacccug      360

guggauagag gcuggggcaa uggcugcggc cuguuuggca agggcagccu cgugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa      480

uaccggauca ugcugagcgu gcacggcagc cagcacuccg gcaugaucgu gaacgacacc      540

ggccacgaga cagacgagaa ccgggccaag guggaaauca cccccaacag cccuagagcc      600

gaggccacac ugggcggcuu uggaucucug ggccuggacu gcgagccuag aaccggccug      660

gauuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaagag      720

ugguuccacg acaucccccu gcccuggcau gccggcgcug auacaggcac accccacugg      780

aacaacaaag aggcucuggu ggaguucaag gacgcccacg ccaagaggca gaccguggug      840

gugcugggau cucaggaagg cgccgugcau acagcucugg cuggcgcccu ggaagccgaa      900

auggauggcg cuaagggcag acuguccagc ggccaccuga agugccggcu gaagauggac      960

aagcugcggc ugaagggcgu guccuacagc cuguguaccg ccgccuucac cuucaccaag     1020

auccccgccg agacacugca cggcaccgug acuguggaag ugcaguacgc cggcaccgac     1080

ggcccuugua aagugccugc ucagauggcu guggacaugc agacccugac cccugugggc     1140

aggcugauca ccgccaaccc ugugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gaacuggacc cacccuucgg cgacagcuac aucgugaucg gcguggggga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuuugaggc uacagugcgg     1320

ggagccaaga gaauggccgu gcugggagau accgccuggg acuuuggcuc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  263
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4817/R4839 ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA 
       Design 1 optimized

<400>  263
gggagaaagc uuaccaugcg gaucaucaac gcccggaaag agaagaagcg cagaggcgcc       60

gauaccagcg ugggcauugu gggccugcug cugacaacag ccauggccgc cgaagugacc      120

agaagaggca gcgccuacua cauguaccug gaccggaacg augccggcga ggccaucagc      180

uuuccaacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugugacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga acccgacgau      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ucaccacaag      360

aagggcgaag ccagacgguc cagacgggcc gugacacugc cuagccacag caccagaaag      420

cugcagaccc ggucccagac cuggcuggaa agcagagagu acaccaagca ccugauccgg      480

guggaaaacu ggaucuuccg gaaccccggc uuugcccugg ccgcugcugc uauugcuugg      540

cugcugggca gcagcaccuc ccagaaagug aucuaccucg ugaugauccu gcugaucgcc      600

ccugccuaca gcauccggug uaucggcgug uccaaccggg acuucgugga aggcaugagc      660

ggcggcacau ggguggacgu ggugcuggaa cauggcggcu gcgugacagu gauggcucag      720

gacaagccca ccguggacau cgagcucgug accaccaccg uguccaauau ggccgaagug      780

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augcccuaca      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgugugcaa gcggacccug      900

guggauagag gcuggggcaa uggcugcggc cuguuuggca agggcagccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa     1020

uaccggauca ugcugagcgu gcacggcagc cagcacuccg gcaugaucgu gaacgacacc     1080

ggccacgaga cagacgagaa ccgggccaag guggaaauca cccccaacag cccuagagcc     1140

gaggccacac ugggcggcuu uggaucucug ggccuggacu gcgagccuag aaccggccug     1200

gauuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaagag     1260

ugguuccacg acaucccccu gcccuggcau gccggcgcug auacaggcac accccacugg     1320

aacaacaaag aggcucuggu ggaguucaag gacgcccacg ccaagaggca gaccguggug     1380

gugcugggau cucaggaagg cgccgugcau acagcucugg cuggcgcccu ggaagccgaa     1440

auggauggcg cuaagggcag acuguccagc ggccaccuga agugccggcu gaagauggac     1500

aagcugcggc ugaagggcgu guccuacagc cuguguaccg ccgccuucac cuucaccaag     1560

auccccgccg agacacugca cggcaccgug acuguggaag ugcaguacgc cggcaccgac     1620

ggcccuugua aagugccugc ucagauggcc guggauaugc agacccugac cccugugggc     1680

aggcugauca ccgccaaccc ugugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gaacuggacc cacccuucgg cgacagcuac aucgugaucg gcguggggga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuuugaggc uacagugcgg     1860

ggagccaaga gaauggccgu gcugggagau accgccuggg acuuuggcuc ugugggcgga     1920

gcccugaacu cucugggcaa gggaauccac cagaucuucg gagccgccuu uaagagccug     1980

uucggcggca uguccugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucucccug augugccugg cucugggagg cgugcugauc     2100

uuccugagca cagccguguc ugccgacgug ggcugcagcg uggacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  264
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 
       optimized

<400>  264
gggagaaagc uuaccauguc ccagaaagug aucuaccucg ugaugauccu gcugaucgcc       60

ccugccuaca gcauccggug uaucggcgug uccaaccggg acuucgugga aggcaugagc      120

ggcggcacau ggguggacgu ggugcuggaa cauggcggcu gcgugacagu gauggcucag      180

gacaagccca ccguggacau cgagcucgug accaccaccg uguccaauau ggccgaagug      240

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augcccuaca      300

cagggcgagg ccuaccugga caagcagucc gacacccagu acgugugcaa gcggacccug      360

guggauagag gcuggggcaa uggcugcggc cuguuuggca agggcagccu cgugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa      480

uaccggauca ugcugagcgu gcacggcagc cagcacuccg gcaugaucgu gaacgacacc      540

ggccacgaga cagacgagaa ccgggccaag guggaaauca cccccaacag cccuagagcc      600

gaggccacac ugggcggcuu uggaucucug ggccuggacu gcgagccuag aaccggccug      660

gauuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaagag      720

ugguuccacg acaucccccu gcccuggcau gccggcgcug auacaggcac accccacugg      780

aacaacaaag aggcucuggu ggaguucaag gacgcccacg ccaagaggca gaccguggug      840

gugcugggau cucaggaagg cgccgugcau acagcucugg cuggcgcccu ggaagccgaa      900

auggauggcg cuaagggcag acuguccagc ggccaccuga agugccggcu gaagauggac      960

aagcugcggc ugaagggcgu guccuacagc cuguguaccg ccgccuucac cuucaccaag     1020

auccccgccg agacacugca cggcaccgug acuguggaag ugcaguacgc cggcaccgac     1080

ggcccuugua aagugccugc ucagauggcc guggauaugc agacccugac cccugugggc     1140

aggcugauca ccgccaaccc ugugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gaacuggacc cacccuucgg cgacagcuac aucgugaucg gcguggggga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuuugaggc uacagugcgg     1320

ggagccaaga gaauggccgu gcugggagau accgccuggg acuuuggcuc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  265
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4821/R4843 ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 
       1 optimized

<400>  265
gggagaaagc uuaccaugcg gaucaucaac gcccggaaag agcggaagcg gagaggcgcc       60

gauaccagca ucggcaucau cggccugcug cugaccacag ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uuugccacaa cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga acccgacgau      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ucaccacaag      360

aagggcgagg ccagacgguc uagaagggcc gugacacugc cuagccacag cacccggaag      420

cugcagacca gaagccagac cuggcuggaa agcagagagu acaccaagca ccugaucaag      480

guggaaaacu ggaucuuccg gaaccccggc uucgcccugg uggccguggc uauugcuugg      540

cugcugggca gcuccaccuc ccagaaagug aucuaccucg ugaugauccu gcugaucgcc      600

ccugccuaca gcauccggug uaucggcgug uccaaccggg acuucgugga aggcaugagc      660

ggcggcacau ggguggacgu ggugcuggaa cauggcggcu gcgugacagu gauggcccag      720

gacaagccca ccguggacau cgagcucgug accaccaccg uguccaauau ggccgaagug      780

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augcccuaca      840

cagggggagg ccuaccugga uaagcagucc gacacccagu acgugugcaa gcggacccug      900

guggauagag gcuggggcaa uggcugcggc cuguuuggca agggcagccu cgugaccugc      960

gccaaguuca ccuguagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa     1020

uaccggauca ugcugagcgu gcacggcagc cagcacagcg gaaugaucgg cuacgagaca     1080

gacgaggacc gggccaaagu ggaagugacc cccaacagcc cuagagccga ggccacacug     1140

ggcggcuuug gaucucuggg ccuggacugc gagccuagaa ccggccugga uuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac     1260

aucccccugc ccuggcaugc cggcgcugau acaggcacac cccacuggaa caacaaagag     1320

gcucuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcucu     1380

caggaaggcg ccgugcauac agcucuggcu ggcgcccugg aagccgaaau ggauggcgcc     1440

aagggcagac uguucagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug     1500

aagggcgugu ccuacagccu guguaccgcc gccuucaccu ucaccaaggu gcccgccgaa     1560

acccugcacg gcacagugac uguggaagug caguacgccg gcaccgacgg cccuugcaag     1620

aucccugugc agauggccgu ggauaugcag acccugaccc cugugggcag gcugaucacc     1680

gccaacccug ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca     1740

cccuucggcg acagcuacau cgugaucggc gugggagaca agaagaucac ccaccacugg     1800

caccgcagcg gcagcacaau cggaaaggcc uucgaagcca cagugcgggg agccaagaga     1860

auggccgugc ugggagauac cgccugggac uuuggcucug ugggcggcgu guucaacucc     1920

cugggcaagg gaauccacca gaucuucgga gccgccuuua agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcucgugu ggcugggccu gaacaccaag     2040

aacggcagca ucucccugac cugccuggcu cugggcggag ugaugaucuu ccugagcacc     2100

gccguguccg ccgauguggg cuguagcgug gacuucagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  266
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 optimized

<400>  266
gggagaaagc uuaccauguc ccagaaagug aucuaccucg ugaugauccu gcugaucgcc       60

ccugccuaca gcauccggug uaucggcgug uccaaccggg acuucgugga aggcaugagc      120

ggcggcacau ggguggacgu ggugcuggaa cauggcggcu gcgugacagu gauggcccag      180

gacaagccca ccguggacau cgagcucgug accaccaccg uguccaauau ggccgaagug      240

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augcccuaca      300

cagggggagg ccuaccugga uaagcagucc gacacccagu acgugugcaa gcggacccug      360

guggauagag gcuggggcaa uggcugcggc cuguuuggca agggcagccu cgugaccugc      420

gccaaguuca ccuguagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa      480

uaccggauca ugcugagcgu gcacggcagc cagcacagcg gaaugaucgg cuacgagaca      540

gacgaggacc gggccaaagu ggaagugacc cccaacagcc cuagagccga ggccacacug      600

ggcggcuuug gaucucuggg ccuggacugc gagccuagaa ccggccugga uuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac      720

aucccccugc ccuggcaugc cggcgcugau acaggcacac cccacuggaa caacaaagag      780

gcucuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcucu      840

caggaaggcg ccgugcauac agcucuggcu ggcgcccugg aagccgaaau ggauggcgcc      900

aagggcagac uguucagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug      960

aagggcgugu ccuacagccu guguaccgcc gccuucaccu ucaccaaggu gcccgccgaa     1020

acccugcacg gcacagugac uguggaagug caguacgccg gcaccgacgg cccuugcaag     1080

aucccugugc agauggccgu ggauaugcag acccugaccc cugugggcag gcugaucacc     1140

gccaacccug ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca     1200

cccuucggcg acagcuacau cgugaucggc gugggagaca agaagaucac ccaccacugg     1260

caccgcagcg gcagcacaau cggaaaggcc uucgaagcca cagugcgggg agccaagaga     1320

auggccgugc ugggagauac cgccugggac uuuggcucuu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  267
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4806/R4836 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 1 optimized

<400>  267
gggagaaagc uuaccaugcg gaucaucaac gcccggaaag agaagaagcg cagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugacaaccg ccauggccgc cgaagugacc      120

agacggggca gcgccuacua cauguaccug gaccggaacg acgccggcga ggccaucagc      180

uuucccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga acccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ucaccacaag      360

aagggcgagg ccagacgguc cagacgggcc gugacacugc ccagccacag cacccggaag      420

cugcagacca gaagccagac cuggcuggaa agcagagagu acaccaagca ccugauccgg      480

guggaaaacu ggaucuuccg gaaccccggc uucgcccugg ccgcugccgc uauugcuugg      540

cugcugggca gcagcaccag ccagaaagug aucuaccugg ugaugauccu gcugaucgcc      600

ccugccuaca gcauccggug caucggcgug uccaaccggg acuucgugga aggcaugagc      660

ggcggcacau ggguggacau cgugcuggaa cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggauau cgagcuggug acaaccaccg uguccaacau ggccgaagug      780

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcggacccug      900

guggacagag gcuggggcaa cggcugcggc cuguuuggca agggcagccu ggugacaugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa     1020

uaccggauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga cagacgagaa ccgggccaag guggaaauca cccccaacag ccccagagcc     1140

gaggccacac ugggcggcuu uggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaagag     1260

ugguuccacg acaucccccu gcccuggcac gcuggcgccg auacaggcac cccucacugg     1320

aacaacaaag aggcccuggu cgaguucaag gacgcccacg ccaagcggca gaccguggug     1380

gugcugggau cucaggaagg cgccguccac acagcccugg cuggcgcccu ggaagccgag     1440

auggauggcg ccaagggcag gcugagcagc ggccaccuga agugccggcu gaagauggac     1500

aagcugcggc ugaagggcgu guccuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacacugca cggcaccgug accguggaag ugcaguacgc cggcaccgac     1620

ggcccuugua aggugccagc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

cggcugauca ccgccaaccc ugugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gaacuggacc cacccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc cacagugcgg     1860

ggagccaaga gaauggccgu gcugggcgac accgccuggg acuuuggauc ugugggcgga     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gagccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag cauuagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug ggcugcagcg uggacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  268
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 
       optimized

<400>  268
gggagaaagc uuaccaugag ccagaaagug aucuaccugg ugaugauccu gcugaucgcc       60

ccugccuaca gcauccggug caucggcgug uccaaccggg acuucgugga aggcaugagc      120

ggcggcacau ggguggacau cgugcuggaa cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggauau cgagcuggug acaaccaccg uguccaacau ggccgaagug      240

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcggacccug      360

guggacagag gcuggggcaa cggcugcggc cuguuuggca agggcagccu ggugacaugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa      480

uaccggauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga cagacgagaa ccgggccaag guggaaauca cccccaacag ccccagagcc      600

gaggccacac ugggcggcuu uggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaagag      720

ugguuccacg acaucccccu gcccuggcac gcuggcgccg auacaggcac cccucacugg      780

aacaacaaag aggcccuggu cgaguucaag gacgcccacg ccaagcggca gaccguggug      840

gugcugggau cucaggaagg cgccguccac acagcccugg cuggcgcccu ggaagccgag      900

auggauggcg ccaagggcag gcugagcagc ggccaccuga agugccggcu gaagauggac      960

aagcugcggc ugaagggcgu guccuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacacugca cggcaccgug accguggaag ugcaguacgc cggcaccgac     1080

ggcccuugua aggugccagc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

cggcugauca ccgccaaccc ugugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gaacuggacc cacccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc cacagugcgg     1320

ggagccaaga gaauggccgu gcugggcgac accgccuggg acuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  269
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4818/R4840 ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA 
       Design 1 optimized

<400>  269
gggagaaagc uuaccaugcg gaucaucaac gcccggaaag agaagaagcg cagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugacaaccg ccauggccgc cgaagugacc      120

agacggggca gcgccuacua cauguaccug gaccggaacg acgccggcga ggccaucagc      180

uuucccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugugacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga acccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ucaccacaag      360

aagggcgagg ccagacgguc cagacgggcc gugacacugc ccagccacag cacccggaag      420

cugcagacca gaagccagac cuggcuggaa agcagagagu acaccaagca ccugauccgg      480

guggaaaacu ggaucuuccg gaaccccggc uucgcccugg ccgcugccgc uauugcuugg      540

cugcugggca gcagcaccag ccagaaagug aucuaccugg ugaugauccu gcugaucgcc      600

ccugccuaca gcauccggug caucggcgug uccaaccggg acuucgugga aggcaugagc      660

ggcggcacau ggguggacgu ggugcuggaa cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug acaaccaccg uguccaacau ggccgaagug      780

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcggacccug      900

guggacagag gcuggggcaa cggcugcggc cuguuuggca agggcagccu ggugacaugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa     1020

uaccggauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga cagacgagaa ccgggccaag guggaaauca cccccaacag ccccagagcc     1140

gaggccacac ugggcggcuu uggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaagag     1260

ugguuccacg acaucccccu gcccuggcac gcuggcgccg auacaggcac cccucacugg     1320

aacaacaaag aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccguggug     1380

gugcugggau cucaggaagg cgccgugcac acagcccugg cuggcgcccu ggaagccgag     1440

auggauggcg ccaagggcag gcugagcagc ggccaccuga agugccggcu gaagauggac     1500

aagcugcggc ugaagggcgu guccuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacacugca cggcaccgug accguggaag ugcaguacgc cggcaccgac     1620

ggcccuugca aggugccagc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

cggcugauca ccgccaaccc ugugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gaacuggacc cacccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc cacagugcgg     1860

ggagccaaga gaauggccgu gcugggcgac accgccuggg acuuuggauc ugugggcgga     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gagccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug ggcugcagcg uggacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  270
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 
       optimized

<400>  270
gggagaaagc uuaccaugag ccagaaagug aucuaccugg ugaugauccu gcugaucgcc       60

ccugccuaca gcauccggug caucggcgug uccaaccggg acuucgugga aggcaugagc      120

ggcggcacau ggguggacgu ggugcuggaa cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug acaaccaccg uguccaacau ggccgaagug      240

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcggacccug      360

guggacagag gcuggggcaa cggcugcggc cuguuuggca agggcagccu ggugacaugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa      480

uaccggauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga cagacgagaa ccgggccaag guggaaauca cccccaacag ccccagagcc      600

gaggccacac ugggcggcuu uggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaagag      720

ugguuccacg acaucccccu gcccuggcac gcuggcgccg auacaggcac cccucacugg      780

aacaacaaag aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccguggug      840

gugcugggau cucaggaagg cgccgugcac acagcccugg cuggcgcccu ggaagccgag      900

auggauggcg ccaagggcag gcugagcagc ggccaccuga agugccggcu gaagauggac      960

aagcugcggc ugaagggcgu guccuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacacugca cggcaccgug accguggaag ugcaguacgc cggcaccgac     1080

ggcccuugca aggugccagc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

cggcugauca ccgccaaccc ugugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gaacuggacc cacccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc cacagugcgg     1320

ggagccaaga gaauggccgu gcugggcgac accgccuggg acuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  271
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4822/R4844 ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 
       1 optimized

<400>  271
gggagaaagc uuaccaugcg gaucaucaac gcccggaaag agcggaagcg gagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agacggggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga acccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ucaccacaag      360

aagggcgagg ccagacgguc cagacgggcc gugacacugc ccagccacag cacccggaag      420

cugcagacca gaagccagac cuggcuggaa agcagagagu acaccaagca ccugaucaag      480

guggaaaacu ggaucuuccg gaaccccggc uucgcccugg uggccguggc uauugccugg      540

cugcugggca gcagcaccag ccagaaagug aucuaccugg ugaugauccu gcugaucgcc      600

ccugccuaca gcauccggug caucggcgug uccaaccggg acuucgugga aggcaugagc      660

ggcggcacau ggguggacgu ggugcuggaa cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug acaaccaccg uguccaacau ggccgaagug      780

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcggacccug      900

guggacagag gcuggggcaa cggcugcggc cuguuuggca agggcagccu ggugacaugc      960

gccaaguuca ccuguagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa     1020

uaccggauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagaca     1080

gacgaggacc gggccaaggu ggaagugacc cccaacagcc cuagagccga ggccacacug     1140

ggcggcuuug gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac     1260

aucccccugc ccuggcacgc uggcgccgau acaggcaccc cucacuggaa caacaaagag     1320

gcccuggugg aguucaagga cgcccacgcc aagcggcaga ccgugguggu gcugggaucu     1380

caggaaggcg ccgugcacac agcccuggcu ggcgcccugg aagccgagau ggauggcgcc     1440

aagggccggc uguucagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug     1500

aagggcgugu ccuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acacugcacg gcaccgugac cguggaagug caguacgccg gcaccgacgg ccccugcaag     1620

aucccugugc agauggccgu ggacaugcag acccugaccc ccgugggccg gcugaucacc     1680

gccaacccug ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca cagugcgggg agccaagaga     1860

auggccgugc ugggcgacac cgccugggau uuuggcuccg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucgga gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggucu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggag ugaugaucuu ccugagcacc     2100

gccgugucug ccgacguggg cugcagcgug gacuucucca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  272
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 optimized

<400>  272
gggagaaagc uuaccaugag ccagaaagug aucuaccugg ugaugauccu gcugaucgcc       60

ccugccuaca gcauccggug caucggcgug uccaaccggg acuucgugga aggcaugagc      120

ggcggcacau ggguggacgu ggugcuggaa cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug acaaccaccg uguccaacau ggccgaagug      240

cggagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcggacccug      360

guggacagag gcuggggcaa cggcugcggc cuguuuggca agggcagccu ggugacaugc      420

gccaaguuca ccuguagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggaa      480

uaccggauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagaca      540

gacgaggacc gggccaaggu ggaagugacc cccaacagcc cuagagccga ggccacacug      600

ggcggcuuug gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac      720

aucccccugc ccuggcacgc uggcgccgau acaggcaccc cucacuggaa caacaaagag      780

gcccuggugg aguucaagga cgcccacgcc aagcggcaga ccgugguggu gcugggaucu      840

caggaaggcg ccgugcacac agcccuggcu ggcgcccugg aagccgagau ggauggcgcc      900

aagggccggc uguucagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug      960

aagggcgugu ccuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acacugcacg gcaccgugac cguggaagug caguacgccg gcaccgacgg ccccugcaag     1080

aucccugugc agauggccgu ggacaugcag acccugaccc ccgugggccg gcugaucacc     1140

gccaacccug ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca cagugcgggg agccaagaga     1320

auggccgugc ugggcgacac cgccugggau uuuggcuccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  273
<211>  10489
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein mRNA Design 1 
       optimized

<400>  273
gggagaaagc uuaccaugaa gaaccccaag aagaagagcg gcggcuuccg caucgugaac       60

augcugaagc gcggcguggc ccgcgugagc cccuucggcg gccugaagcg ccugcccgcc      120

ggccugcugc ugggccacgg ccccauccgc auggugcugg ccauccuggc cuuccugcgc      180

uucaccgcca ucaagcccag ccugggccug aucaaccgcu ggggcagcgu gggcaagaag      240

gaggccaugg agaucaucaa gaaguucaag aaggaccugg ccgccaugcu gcgcaucauc      300

aacgcccgca aggagaagaa gcgccgcggc gccgacacca gcgugggcau cgugggccug      360

cugcugacca ccgccauggc cgccgaggug acccgccgcg gcagcgccua cuacauguac      420

cuggaccgca acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      480

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      540

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      600

accugggugg uguacggcac cugccaccac aagaagggcg aggcccgccg cagccgccgc      660

gccgugaccc ugcccagcca cagcacccgc aagcugcaga cccgcagcca gaccuggcug      720

gagagccgcg aguacaccaa gcaccugauc cgcguggaga acuggaucuu ccgcaacccc      780

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      840

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcauccg cugcaucggc      900

gugagcaacc gcgacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      960

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug     1020

gugaccacca ccgugagcaa cauggccgag gugcgcagcu acugcuacga ggccagcauc     1080

agcgacaugg ccagcgacag ccgcugcccc acccagggcg aggccuaccu ggacaagcag     1140

agcgacaccc aguacgugug caagcgcacc cugguggacc gcggcugggg caacggcugc     1200

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug     1260

accggcaaga gcauccagcc cgagaaccug gaguaccgca ucaugcugag cgugcacggc     1320

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaaccgcgcc     1380

aagguggaga ucacccccaa cagcccccgc gccgaggcca cccugggcgg cuucggcagc     1440

cugggccugg acugcgagcc ccgcaccggc cuggacuuca gcgaccugua cuaccugacc     1500

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1560

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1620

aaggacgccc acgccaagcg ccagaccgug guggugcugg gcagccagga gggcgccgug     1680

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg ccgccugagc     1740

agcggccacc ugaagugccg ccugaagaug gacaagcugc gccugaaggg cgugagcuac     1800

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1860

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1920

gccguggaca ugcagacccu gacccccgug ggccgccuga ucaccgccaa ccccgugauc     1980

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     2040

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcaccg cagcggcagc     2100

accaucggca aggccuucga ggccaccgug cgcggcgcca agcgcauggc cgugcugggc     2160

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     2220

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     2280

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2340

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2400

gugggcugca gcguggacuu cagcaagaag gagacccgcu gcggcaccgg cguguucgug     2460

uacaacgacg uggaggccug gcgcgaccgc uacaaguacc accccgacag cccccgccgc     2520

cuggccgccg ccgugaagca ggccugggag gacggcaucu gcggcaucag cagcgugagc     2580

cgcauggaga acaucaugug gcgcagcgug gagggcgagc ugaacgccau ccuggaggag     2640

aacggcgugc agcugaccgu gguggugggc agcgugaaga accccaugug gcgcggcccc     2700

cagcgccugc ccgugcccgu gaacgagcug ccccacggcu ggaaggccug gggcaagagc     2760

cacuucgugc gcgccgccaa gaccaacaac agcuucgugg uggacggcga cacccugaag     2820

gagugccccc ugaagcaccg cgccuggaac agcuuccugg uggaggacca cggcuucggc     2880

guguuccaca ccagcgugug gcugaaggug cgcgaggacu acagccugga gugcgacccc     2940

gccgugaucg gcaccgccgu gaagggcaag gaggccgugc acagcgaccu gggcuacugg     3000

aucgagagcg agaagaacga caccuggcgc cugaagcgcg cccaccugau cgagaugaag     3060

accugcgagu ggcccaagag ccacacccug uggaccgacg gcaucgagga gagcgaccug     3120

aucaucccca agagccuggc cggcccccug agccaccaca acacccgcga gggcuaccgc     3180

acccagauga agggccccug gcacagcgag gagcuggaga uccgcuucga ggagugcccc     3240

ggcaccaagg ugcacgugga ggagaccugc ggcacccgcg gccccagccu gcgcagcacc     3300

accgccagcg gccgcgugau cgaggagugg ugcugccgcg agugcaccau gcccccccug     3360

agcuuccgcg ccaaggacgg cugcugguac ggcauggaga uccgcccccg caaggagccc     3420

gagagcaacc uggugcgcag cauggugacc gccggcagca ccgaccacau ggaccacuuc     3480

agccugggcg ugcuggugau ccugcugaug gugcaggagg gccugaagaa gcgcaugacc     3540

accaagauca ucaucagcac cagcauggcc gugcuggugg ccaugauccu gggcggcuuc     3600

agcaugagcg accuggccaa gcuggccauc cugaugggcg ccaccuucgc cgagaugaac     3660

accggcggcg acguggccca ccuggcccug aucgccgccu ucaaggugcg ccccgcccug     3720

cuggugagcu ucaucuuccg cgccaacugg accccccgcg agagcaugcu gcuggcccug     3780

gccagcugcc ugcugcagac cgccaucagc gcccuggagg gcgaccugau ggugcugauc     3840

aacggcuucg cccuggccug gcuggccauc cgcgccaugg uggugccccg caccgacaac     3900

aucacccugg ccauccuggc cgcccugacc ccccuggccc gcggcacccu gcugguggcc     3960

uggcgcgccg gccuggccac cugcggcggc uucaugcugc ugagccugaa gggcaagggc     4020

agcgugaaga agaaccugcc cuucgugaug gcccugggcc ugaccgccgu gcgccuggug     4080

gaccccauca acgugguggg ccugcugcug cugacccgca gcggcaagcg cagcuggccc     4140

cccagcgagg ugcugaccgc cgugggccug aucugcgccc uggccggcgg cuucgccaag     4200

gccgacaucg agauggccgg ccccauggcc gccgugggcc ugcugaucgu gagcuacgug     4260

gugagcggca agagcgugga cauguacauc gagcgcgccg gcgacaucac cugggagaag     4320

gacgccgagg ugaccggcaa cagcccccgc cuggacgugg cccuggacga gagcggcgac     4380

uucagccugg uggaggacga cggccccccc augcgcgaga ucauccugaa gguggugcug     4440

augaccaucu gcggcaugaa ccccaucgcc auccccuucg ccgccggcgc cugguacgug     4500

uacgugaaga ccggcaagcg cagcggcgcc cugugggacg ugcccgcccc caaggaggug     4560

aagaagggcg agaccaccga cggcguguac cgcgugauga cccgccgccu gcugggcagc     4620

acccaggugg gcgugggcgu gaugcaggag ggcguguucc acaccaugug gcacgugacc     4680

aagggcagcg cccugcgcag cggcgagggc cgccuggacc ccuacugggg cgacgugaag     4740

caggaccugg ugagcuacug cggccccugg aagcuggacg ccgccuggga cggccacagc     4800

gaggugcagc ugcuggccgu gccccccggc gagcgcgccc gcaacaucca gacccugccc     4860

ggcaucuuca agaccaagga cggcgacauc ggcgccgugg cccuggacua ccccgccggc     4920

accagcggca gccccauccu ggacaagugc ggccgcguga ucggccugua cggcaacggc     4980

guggugauca agaacggcag cuacgugagc gccaucaccc agggccgccg cgaggaggag     5040

acccccgugg agugcuucga gcccagcaug cugaagaaga agcagcugac cgugcuggac     5100

cugcaccccg gcgccggcaa gacccgccgc gugcugcccg agaucgugcg cgaggccauc     5160

aagacccgcc ugcgcaccgu gauccuggcc cccacccgcg ugguggccgc cgagauggag     5220

gaggcccugc gcggccugcc cgugcgcuac augaccaccg ccgugaacgu gacccacagc     5280

ggcaccgaga ucguggaccu gaugugccac gccaccuuca ccagccgccu gcugcagccc     5340

auccgcgugc ccaacuacaa ccuguacauc auggacgagg cccacuucac cgaccccagc     5400

agcaucgccg cccgcggcua caucagcacc cgcguggaga ugggcgaggc cgccgccauc     5460

uucaugaccg ccaccccccc cggcacccgc gacgccuucc ccgacagcaa cagccccauc     5520

auggacaccg agguggaggu gcccgagcgc gccuggagca gcggcuucga cugggugacc     5580

gacuacagcg gcaagaccgu gugguucgug cccagcgugc gcaacggcaa cgagaucgcc     5640

gccugccuga ccaaggccgg caagcgcgug auccagcuga gccgcaagac cuucgagacc     5700

gaguuccaga agaccaagca ccaggagugg gacuucgugg ugaccaccga caucagcgag     5760

augggcgcca acuucaaggc cgaccgcgug aucgacagcc gccgcugccu gaagcccgug     5820

auccuggacg gcgagcgcgu gauccuggcc ggccccaugc ccgugaccca cgccagcgcc     5880

gcccagcgcc gcggccgcau cggccgcaac cccaacaagc ccggcgacga guaccuguac     5940

ggcggcggcu gcgccgagac cgacgaggac cacgcccacu ggcuggaggc ccgcaugcug     6000

cuggacaaca ucuaccugca ggacggccug aucgccagcc uguaccgccc cgaggccgac     6060

aagguggccg ccaucgaggg cgaguucaag cugcgcaccg agcagcgcaa gaccuucgug     6120

gagcugauga agcgcggcga ccugcccgug uggcuggccu accagguggc cagcgccggc     6180

aucaccuaca ccgaccgccg cuggugcuuc gacggcacca ccaacaacac caucauggag     6240

gacagcgugc ccgccgaggu guggacccgc cacggcgaga agcgcgugcu gaagccccgc     6300

uggauggacg cccgcgugug cagcgaccac gccgcccuga agagcuucaa ggaguucgcc     6360

gccggcaagc gcggcgccgc cuucggcgug auggaggccc ugggcacccu gcccggccac     6420

augaccgagc gcuuccagga ggccaucgac aaccuggccg ugcugaugcg cgccgagacc     6480

ggcagccgcc ccuacaaggc cgccgccgcc cagcugcccg agacccugga gaccaucaug     6540

cugcugggcc ugcugggcac cgugagccug ggcaucuucu ucgugcugau gcgcaacaag     6600

ggcaucggca agaugggcuu cggcauggug acccugggcg ccagcgccug gcugaugugg     6660

cugagcgaga ucgagcccgc ccgcaucgcc ugcgugcuga ucgugguguu ccugcugcug     6720

guggugcuga uccccgagcc cgagaagcag cgcagccccc aggacaacca gauggccauc     6780

aucaucaugg uggccguggg ccugcugggc cugaucaccg ccaacgagcu gggcuggcug     6840

gagcgcacca agagcgaccu gagccaccug augggccgcc gcgaggaggg cgccaccaug     6900

ggcuucagca uggacaucga ccugcgcccc gccagcgccu gggccaucua cgccgcccug     6960

accaccuuca ucacccccgc cgugcagcac gccgugacca ccagcuacaa caacuacagc     7020

cugauggcca uggccaccca ggccggcgug cuguucggca ugggcaaggg caugcccuuc     7080

uacgccuggg acuucggcgu gccccugcug augaucggcu gcuacagcca gcugaccccc     7140

cugacccuga ucguggccau cauccugcug guggcccacu acauguaccu gauccccggc     7200

cugcaggccg ccgccgcccg cgccgcccag aagcgcaccg ccgccggcau caugaagaac     7260

cccguggugg acggcaucgu ggugaccgac aucgacacca ugaccaucga cccccaggug     7320

gagaagaaga ugggccaggu gcugcugaug gccguggccg ugagcagcgc cauccugagc     7380

cgcaccgccu ggggcugggg cgaggccggc gcccugauca ccgccgccac cagcacccug     7440

ugggagggca gccccaacaa guacuggaac agcagcaccg ccaccagccu gugcaacauc     7500

uuccgcggca gcuaccuggc cggcgccagc cugaucuaca ccgugacccg caacgccggc     7560

cuggugaagc gccgcggcgg cggcaccggc gagacccugg gcgagaagug gaaggcccgc     7620

cugaaccaga ugagcgcccu ggaguucuac agcuacaaga agagcggcau caccgaggug     7680

ugccgcgagg aggcccgccg cgcccugaag gacggcgugg ccaccggcgg ccacgccgug     7740

agccgcggca gcgccaagcu gcgcuggcug guggagcgcg gcuaccugca gcccuacggc     7800

aaggugaucg accugggcug cggccgcggc ggcuggagcu acuacgccgc caccauccgc     7860

aaggugcagg aggugaaggg cuacaccaag ggcggccccg gccacgagga gcccgugcug     7920

gugcagagcu acggcuggaa caucgugcgc cugaagagcg gcguggacgu guuccacaug     7980

gccgccgagc ccugcgacac ccugcugugc gacaucggcg agagcagcag cagccccgag     8040

guggaggagg cccgcacccu gcgcgugcug agcauggugg gcgacuggcu ggagaagcgc     8100

cccggcgccu ucugcaucaa ggugcugugc cccuacacca gcaccaugau ggagacccug     8160

gagcgccugc agcgccgcua cggcggcggc cuggugcgcg ugccccugag ccgcaacagc     8220

acccacgaga uguacugggu gagcggcgcc aagagcaaca ccaucaagag cgugagcacc     8280

accagccagc ugcugcuggg ccgcauggac ggcccccgcc gccccgugaa guacgaggag     8340

gacgugaacc ugggcagcgg cacccgcgcc guggugagcu gcgccgaggc ccccaacaug     8400

aagaucaucg gcaaccgcau cgagcgcauc cgcagcgagc acgccgagac cugguucuuc     8460

gacgagaacc accccuaccg caccugggcc uaccacggca gcuacgaggc ccccacccag     8520

ggcagcgcca gcagccugau caacggcgug gugcgccugc ugagcaagcc cugggacgug     8580

gugaccggcg ugaccggcau cgccaugacc gacaccaccc ccuacggcca gcagcgcgug     8640

uucaaggaga agguggacac ccgcgugccc gacccccagg agggcacccg ccaggugaug     8700

agcaugguga gcagcuggcu guggaaggag cugggcaagc acaagcgccc ccgcgugugc     8760

accaaggagg aguucaucaa caaggugcgc agcaacgccg cccugggcgc caucuucgag     8820

gaggagaagg aguggaagac cgccguggag gccgugaacg acccccgcuu cugggcccug     8880

guggacaagg agcgcgagca ccaccugcgc ggcgagugcc agagcugcgu guacaacaug     8940

augggcaagc gcgagaagaa gcagggcgag uucggcaagg ccaagggcag ccgcgccauc     9000

ugguacaugu ggcugggcgc ccgcuuccug gaguucgagg cccugggcuu ccugaacgag     9060

gaccacugga ugggccgcga gaacagcggc ggcggcgugg agggccuggg ccugcagcgc     9120

cugggcuacg ugcuggagga gaugagccgc auccccggcg gccgcaugua cgccgacgac     9180

accgccggcu gggacacccg caucagccgc uucgaccugg agaacgaggc ccugaucacc     9240

aaccagaugg agaagggcca ccgcgcccug gcccuggcca ucaucaagua caccuaccag     9300

aacaaggugg ugaaggugcu gcgccccgcc gagaagggca agaccgugau ggacaucauc     9360

agccgccagg accagcgcgg cagcggccag guggugaccu acgcccugaa caccuucacc     9420

aaccuggugg ugcagcugau ccgcaacaug gaggccgagg aggugcugga gaugcaggac     9480

cuguggcugc ugcgccgcag cgagaaggug accaacuggc ugcagagcaa cggcugggac     9540

cgccugaagc gcauggccgu gagcggcgac gacugcgugg ugaagcccau cgacgaccgc     9600

uucgcccacg cccugcgcuu ccugaacgac augggcaagg ugcgcaagga cacccaggag     9660

uggaagccca gcaccggcug ggacaacugg gaggaggugc ccuucugcag ccaccacuuc     9720

aacaagcugc accugaagga cggccgcagc aucguggugc ccugccgcca ccaggacgag     9780

cugaucggcc gcgcccgcgu gagccccggc gccggcugga gcauccgcga gaccgccugc     9840

cuggccaaga gcuacgccca gauguggcag cugcuguacu uccaccgccg cgaccugcgc     9900

cugauggcca acgccaucug cagcagcgug cccguggacu gggugcccac cggccgcacc     9960

accuggagca uccacggcaa gggcgagugg augaccaccg aggacaugcu gguggugugg    10020

aaccgcgugu ggaucgagga gaacgaccac auggaggaca agacccccgu gaccaagugg    10080

accgacaucc ccuaccuggg caagcgcgag gaccuguggu gcggcagccu gaucggccac    10140

cgcccccgca ccaccugggc cgagaacauc aagaacaccg ugaacauggu gcgccgcauc    10200

aucggcgacg aggagaagua cauggacuac cugagcaccc aggugcgcua ccugggcgag    10260

gagggcagca cccccggcgu gcuguaagga cuaguuauaa gacugacuag cccgaugggc    10320

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc    10440

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                10489


<210>  274
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 
       optimized

<400>  274
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

cgccgcggca gcgccuacua cauguaccug gaccgcaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcag ccgccgcgcc gugacccugc ccagccacag cacccgcaag      420

cugcagaccc gcagccagac cuggcuggag agccgcgagu acaccaagca ccugauccgc      480

guggagaacu ggaucuuccg caaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccccgcgcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggccg ccugagcagc ggccaccuga agugccgccu gaagauggac     1500

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  275
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 
       optimized

<400>  275
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

cgccgcggca gcgccuacua cauguaccug gaccgcaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcag ccgccgcgcc gugacccugc ccagccacag cacccgcaag      420

cugcagaccc gcagccagac cuggcuggag agccgcgagu acaccaagca ccugauccgc      480

guggagaacu ggaucuuccg caaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccccgcgcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggccg ccugagcagc ggccaccuga agugccgccu gaagauggac     1500

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caaguaagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  276
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 
       optimized

<400>  276
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      120

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccccgcgcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggccg ccugagcagc ggccaccuga agugccgccu gaagauggac      960

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  277
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 
       optimized

<400>  277
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      120

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccccgcgcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggccg ccugagcagc ggccaccuga agugccgccu gaagauggac      960

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cuaaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  278
<211>  10489
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein mRNA Design 1 
       optimized

<400>  278
gggagaaagc uuaccaugaa gaaccccaag aagaagagcg gcggcuuccg caucgugaac       60

augcugaagc gcggcguggc ccgcgugagc cccuucggcg gccugaagcg ccugcccgcc      120

ggccugcugc ugggccacgg ccccauccgc auggugcugg ccauccuggc cuuccugcgc      180

uucaccgcca ucaagcccag ccugggccug aucaaccgcu ggggcagcgu gggcaagaag      240

gaggccaugg agaucaucaa gaaguucaag aaggaccugg ccgccaugcu gcgcaucauc      300

aacgcccgca aggagaagaa gcgccgcggc gccgacacca gcgugggcau cgugggccug      360

cugcugacca ccgccauggc cgccgaggug acccgccgcg gcagcgccua cuacauguac      420

cuggaccgca acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      480

ugcuacaucc agaucaugga ccugggccac accugcgacg ccaccaugag cuacgagugc      540

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      600

accugggugg uguacggcac cugccaccac aagaagggcg aggcccgccg cagccgccgc      660

gccgugaccc ugcccagcca cagcacccgc aagcugcaga cccgcagcca gaccuggcug      720

gagagccgcg aguacaccaa gcaccugauc cgcguggaga acuggaucuu ccgcaacccc      780

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      840

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcauccg cugcaucggc      900

gugagcaacc gcgacuucgu ggagggcaug agcggcggca ccugggugga cguggugcug      960

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug     1020

gugaccacca ccgugagcaa cauggccgag gugcgcagcu acugcuacga ggccagcauc     1080

agcgacaugg ccagcgacag ccgcugcccc acccagggcg aggccuaccu ggacaagcag     1140

agcgacaccc aguacgugug caagcgcacc cugguggacc gcggcugggg caacggcugc     1200

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug     1260

accggcaaga gcauccagcc cgagaaccug gaguaccgca ucaugcugag cgugcacggc     1320

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaaccgcgcc     1380

aagguggaga ucacccccaa cagcccccgc gccgaggcca cccugggcgg cuucggcagc     1440

cugggccugg acugcgagcc ccgcaccggc cuggacuuca gcgaccugua cuaccugacc     1500

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1560

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1620

aaggacgccc acgccaagcg ccagaccgug guggugcugg gcagccagga gggcgccgug     1680

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg ccgccugagc     1740

agcggccacc ugaagugccg ccugaagaug gacaagcugc gccugaaggg cgugagcuac     1800

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1860

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1920

gccguggaca ugcagacccu gacccccgug ggccgccuga ucaccgccaa ccccgugauc     1980

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     2040

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcaccg cagcggcagc     2100

accaucggca aggccuucga ggccaccgug cgcggcgcca agcgcauggc cgugcugggc     2160

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     2220

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     2280

auccugaucg gcacccugcu gauguggcug ggccugaacg ccaagaacgg cagcaucagc     2340

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2400

gugggcugca gcguggacuu cagcaagaag gagacccgcu gcggcaccgg cguguucgug     2460

uacaacgacg uggaggccug gcgcgaccgc uacaaguacc accccgacag cccccgccgc     2520

cuggccgccg ccgugaagca ggccugggag gacggcaucu gcggcaucag cagcgugagc     2580

cgcauggaga acaucaugug gcgcagcgug gagggcgagc ugaacgccau ccuggaggag     2640

aacggcgugc agcugaccgu gguggugggc agcgugaaga accccaugug gcgcggcccc     2700

cagcgccugc ccgugcccgu gaacgagcug ccccacggcu ggaaggccug gggcaagagc     2760

uacuucgugc gcgccgccaa gaccaacaac agcuucgugg uggacggcga cacccugaag     2820

gagugccccc ugaagcaccg cgccuggaac agcuuccugg uggaggacca cggcuucggc     2880

guguuccaca ccagcgugug gcugaaggug cgcgaggacu acagccugga gugcgacccc     2940

gccgugaucg gcaccgccgu gaagggcaag gaggccgugc acagcgaccu gggcuacugg     3000

aucgagagcg agaagaacga caccuggcgc cugaagcgcg cccaccugau cgagaugaag     3060

accugcgagu ggcccaagag ccacacccug uggaccgacg gcaucgagga gagcgaccug     3120

aucaucccca agagccuggc cggcccccug agccaccaca acacccgcga gggcuaccgc     3180

acccagauga agggccccug gcacagcgag gagcuggaga uccgcuucga ggagugcccc     3240

ggcaccaagg ugcacgugga ggagaccugc ggcacccgcg gccccagccu gcgcagcacc     3300

accgccagcg gccgcgugau cgaggagugg ugcugccgcg agugcaccau gcccccccug     3360

agcuuccgcg ccaaggacgg cugcugguac ggcauggaga uccgcccccg caaggagccc     3420

gagagcaacc uggugcgcag cauggugacc gccggcagca ccgaccacau ggaccacuuc     3480

agccugggcg ugcuggugau ccugcugaug gugcaggagg gccugaagaa gcgcaugacc     3540

accaagauca ucaucagcac cagcauggcc gugcuggugg ccaugauccu gggcggcuuc     3600

agcaugagcg accuggccaa gcuggccauc cugaugggcg ccaccuucgc cgagaugaac     3660

accggcggcg acguggccca ccuggcccug aucgccgccu ucaaggugcg ccccgcccug     3720

cuggugagcu ucaucuuccg cgccaacugg accccccgcg agagcaugcu gcuggcccug     3780

gccagcugcc ugcugcagac cgccaucagc gcccuggagg gcgaccugau ggugcugauc     3840

aacggcuucg cccuggccug gcuggccauc cgcgccaugg uggugccccg caccgacaac     3900

aucacccugg ccauccuggc cgcccugacc ccccuggccc gcggcacccu gcugguggcc     3960

uggcgcgccg gccuggccac cugcggcggc uucaugcugc ugagccugaa gggcaagggc     4020

agcgugaaga agaaccugcc cuucgugaug gcccugggcc ugaccgccgu gcgccuggug     4080

gaccccauca acgugguggg ccugcugcug cugacccgca gcggcaagcg cagcuggccc     4140

cccagcgagg ugcugaccgc cgugggccug aucugcgccc uggccggcgg cuucgccaag     4200

gccgacaucg agauggccgg ccccauggcc gccgugggcc ugcugaucgu gagcuacgug     4260

gugagcggca agagcgugga cauguacauc gagcgcgccg gcgacaucac cugggagaag     4320

gacgccgagg ugaccggcaa cagcccccgc cuggacgugg cccuggacga gagcggcgac     4380

uucagccugg uggaggacga cggccccccc augcgcgaga ucauccugaa gguggugcug     4440

augaccaucu gcggcaugaa ccccaucgcc auccccuucg ccgccggcgc cugguacgug     4500

uacgugaaga ccggcaagcg cagcggcgcc cugugggacg ugcccgcccc caaggaggug     4560

aagaagggcg agaccaccga cggcguguac cgcgugauga cccgccgccu gcugggcagc     4620

acccaggugg gcgugggcgu gaugcaggag ggcguguucc acaccaugug gcacgugacc     4680

aagggcagcg cccugcgcag cggcgagggc cgccuggacc ccuacugggg cgacgugaag     4740

caggaccugg ugagcuacug cggccccugg aagcuggacg ccgccuggga cggccacagc     4800

gaggugcagc ugcuggccgu gccccccggc gagcgcgccc gcaacaucca gacccugccc     4860

ggcaucuuca agaccaagga cggcgacauc ggcgccgugg cccuggacua ccccgccggc     4920

accagcggca gccccauccu ggacaagugc ggccgcguga ucggccugua cggcaacggc     4980

guggugauca agaacggcag cuacgugagc gccaucaccc agggccgccg cgaggaggag     5040

acccccgugg agugcuucga gcccagcaug cugaagaaga agcagcugac cgugcuggac     5100

cugcaccccg gcgccggcaa gacccgccgc gugcugcccg agaucgugcg cgaggccauc     5160

aagacccgcc ugcgcaccgu gauccuggcc cccacccgcg ugguggccgc cgagauggag     5220

gaggcccugc gcggccugcc cgugcgcuac augaccaccg ccgugaacgu gacccacagc     5280

ggcaccgaga ucguggaccu gaugugccac gccaccuuca ccagccgccu gcugcagccc     5340

auccgcgugc ccaacuacaa ccuguacauc auggacgagg cccacuucac cgaccccagc     5400

agcaucgccg cccgcggcua caucagcacc cgcguggaga ugggcgaggc cgccgccauc     5460

uucaugaccg ccaccccccc cggcacccgc gacgccuucc ccgacagcaa cagccccauc     5520

auggacaccg agguggaggu gcccgagcgc gccuggagca gcggcuucga cugggugacc     5580

gaccacagcg gcaagaccgu gugguucgug cccagcgugc gcaacggcaa cgagaucgcc     5640

gccugccuga ccaaggccgg caagcgcgug auccagcuga gccgcaagac cuucgagacc     5700

gaguuccaga agaccaagca ccaggagugg gacuucgugg ugaccaccga caucagcgag     5760

augggcgcca acuucaaggc cgaccgcgug aucgacagcc gccgcugccu gaagcccgug     5820

auccuggacg gcgagcgcgu gauccuggcc ggccccaugc ccgugaccca cgccagcgcc     5880

gcccagcgcc gcggccgcau cggccgcaac cccaacaagc ccggcgacga guaccuguac     5940

ggcggcggcu gcgccgagac cgacgaggac cacgcccacu ggcuggaggc ccgcaugcug     6000

cuggacaaca ucuaccugca ggacggccug aucgccagcc uguaccgccc cgaggccgac     6060

aagguggccg ccaucgaggg cgaguucaag cugcgcaccg agcagcgcaa gaccuucgug     6120

gagcugauga agcgcggcga ccugcccgug uggcuggccu accagguggc cagcgccggc     6180

aucaccuaca ccgaccgccg cuggugcuuc gacggcacca ccaacaacac caucauggag     6240

gacagcgugc ccgccgaggu guggacccgc cacggcgaga agcgcgugcu gaagccccgc     6300

uggauggacg cccgcgugug cagcgaccac gccgcccuga agagcuucaa ggaguucgcc     6360

gccggcaagc gcggcgccgc cuucggcgug auggaggccc ugggcacccu gcccggccac     6420

augaccgagc gcuuccagga ggccaucgac aaccuggccg ugcugaugcg cgccgagacc     6480

ggcagccgcc ccuacaaggc cgccgccgcc cagcugcccg agacccugga gaccaucaug     6540

cugcugggcc ugcugggcac cgugagccug ggcaucuucu ucgugcugau gcgcaacaag     6600

ggcaucggca agaugggcuu cggcauggug acccugggcg ccagcgccug gcugaugugg     6660

cugagcgaga ucgagcccgc ccgcaucgcc ugcgugcuga ucgugguguu ccugcugcug     6720

guggugcuga uccccgagcc cgagaagcag cgcagccccc aggacaacca gauggccauc     6780

aucaucaugg uggccguggg ccugcugggc cugaucaccg ccaacgagcu gggcuggcug     6840

gagcgcacca agagcgaccu gagccaccug augggccgcc gcgaggaggg cgccaccauc     6900

ggcuucagca uggacaucga ccugcgcccc gccagcgccu gggccaucua cgccgcccug     6960

accaccuuca ucacccccgc cgugcagcac gccgugacca ccagcuacaa caacuacagc     7020

cugauggcca uggccaccca ggccggcgug cuguucggca ugggcaaggg caugcccuuc     7080

uacgccuggg acuucggcgu gccccugcug augaucggcu gcuacagcca gcugaccccc     7140

cugacccuga ucguggccau cauccugcug guggcccacu acauguaccu gauccccggc     7200

cugcaggccg ccgccgcccg cgccgcccag aagcgcaccg ccgccggcau caugaagaac     7260

cccguggugg acggcaucgu ggugaccgac aucgacacca ugaccaucga cccccaggug     7320

gagaagaaga ugggccaggu gcugcugauc gccguggccg ugagcagcgc cauccugagc     7380

cgcaccgccu ggggcugggg cgaggccggc gcccugauca ccgccgccac cagcacccug     7440

ugggagggca gccccaacaa guacuggaac agcagcaccg ccaccagccu gugcaacauc     7500

uuccgcggca gcuaccuggc cggcgccagc cugaucuaca ccgugacccg caacgccggc     7560

cuggugaagc gccgcggcgg cggcaccggc gagacccugg gcgagaagug gaaggcccgc     7620

cugaaccaga ugagcgcccu ggaguucuac agcuacaaga agagcggcau caccgaggug     7680

ugccgcgagg aggcccgccg cgcccugaag gacggcgugg ccaccggcgg ccacgccgug     7740

agccgcggca gcgccaagcu gcgcuggcug guggagcgcg gcuaccugca gcccuacggc     7800

aaggugaucg accugggcug cggccgcggc ggcuggagcu acuacgccgc caccauccgc     7860

aaggugcagg aggugaaggg cuacaccaag ggcggccccg gccacgagga gcccgugcug     7920

gugcagagcu acggcuggaa caucgugcgc cugaagagcg gcguggacgu guuccacaug     7980

gccgccgagc ccugcgacac ccugcugugc gacaucggcg agagcagcag cagccccgag     8040

guggaggagg cccgcacccu gcgcgugcug agcauggugg gcgacuggcu ggagaagcgc     8100

cccggcgccu ucugcaucaa ggugcugugc cccuacacca gcaccaugau ggagacccug     8160

gagcgccugc agcgccgcua cggcggcggc cuggugcgcg ugccccugag ccgcaacagc     8220

acccacgaga uguacugggu gagcggcgcc aagagcaaca ccaucaagag cgugagcacc     8280

accagccagc ugcugcuggg ccgcauggac ggcccccgcc gccccgugaa guacgaggag     8340

gacgugaacc ugggcagcgg cacccgcgcc guggugagcu gcgccgaggc ccccaacaug     8400

aagaucaucg gcaaccgcau cgagcgcauc cgcagcgagc acgccgagac cugguucuuc     8460

gacgagaacc accccuaccg caccugggcc uaccacggca gcuacgaggc ccccacccag     8520

ggcagcgcca gcagccugau caacggcgug gugcgccugc ugagcaagcc cugggacgug     8580

gugaccggcg ugaccggcau cgccaugacc gacaccaccc ccuacggcca gcagcgcgug     8640

uucaaggaga agguggacac ccgcgugccc gacccccagg agggcacccg ccaggugaug     8700

agcaugguga gcagcuggcu guggaaggag cugggcaagc acaagcgccc ccgcgugugc     8760

accaaggagg aguucaucaa caaggugcgc agcaacgccg cccugggcgc caucuucgag     8820

gaggagaagg aguggaagac cgccguggag gccgugaacg acccccgcuu cugggcccug     8880

guggacaagg agcgcgagca ccaccugcgc ggcgagugcc agagcugcgu guacaacaug     8940

augggcaagc gcgagaagaa gcagggcgag uucggcaagg ccaagggcag ccgcgccauc     9000

ugguacaugu ggcugggcgc ccgcuuccug gaguucgagg cccugggcuu ccugaacgag     9060

gaccacugga ugggccgcga gaacagcggc ggcggcgugg agggccuggg ccugcagcgc     9120

cugggcuacg ugcuggagga gaugagccgc auccccggcg gccgcaugua cgccgacgac     9180

accgccggcu gggacacccg caucagccgc uucgaccugg agaacgaggc ccugaucacc     9240

aaccagaugg agaagggcca ccgcgcccug gcccuggcca ucaucaagua caccuaccag     9300

aacaaggugg ugaaggugcu gcgccccgcc gagaagggca agaccgugau ggacaucauc     9360

agccgccagg accagcgcgg cagcggccag guggugaccu acgcccugaa caccuucacc     9420

aaccuggugg ugcagcugau ccgcaacaug gaggccgagg aggugcugga gaugcaggac     9480

cuguggcugc ugcgccgcag cgagaaggug accaacuggc ugcagagcaa cggcugggac     9540

cgccugaagc gcauggccgu gagcggcgac gacugcgugg ugaagcccau cgacgaccgc     9600

uucgcccacg cccugcgcuu ccugaacgac augggcaagg ugcgcaagga cacccaggag     9660

uggaagccca gcaccggcug ggacaacugg gaggaggugc ccuucugcag ccaccacuuc     9720

aacaagcugc accugaagga cggccgcagc aucguggugc ccugccgcca ccaggacgag     9780

cugaucggcc gcgcccgcgu gagccccggc gccggcugga gcauccgcga gaccgccugc     9840

cuggccaaga gcuacgccca gauguggcag cugcuguacu uccaccgccg cgaccugcgc     9900

cugauggcca acgccaucug cagcagcgug cccguggacu gggugcccac cggccgcacc     9960

accuggagca uccacggcaa gggcgagugg augaccaccg aggacaugcu gguggugugg    10020

aaccgcgugu ggaucgagga gaacgaccac auggaggaca agacccccgu gaccaagugg    10080

accgacaucc ccuaccuggg caagcgcgag gaccuguggu gcggcagccu gaucggccac    10140

cgcccccgca ccaccugggc cgagaacauc aagaacaccg ugaacauggu gcgccgcauc    10200

aucggcgacg aggagaagua cauggacuac cugagcaccc aggugcgcua ccugggcgag    10260

gagggcagca cccccggcgu gcuguaagga cuaguuauaa gacugacuag cccgaugggc    10320

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc    10440

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                10489


<210>  279
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 
       optimized

<400>  279
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

cgccgcggca gcgccuacua cauguaccug gaccgcaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcag ccgccgcgcc gugacccugc ccagccacag cacccgcaag      420

cugcagaccc gcagccagac cuggcuggag agccgcgagu acaccaagca ccugauccgc      480

guggagaacu ggaucuuccg caaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccccgcgcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggccg ccugagcagc ggccaccuga agugccgccu gaagauggac     1500

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  280
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 
       optimized

<400>  280
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

cgccgcggca gcgccuacua cauguaccug gaccgcaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcag ccgccgcgcc gugacccugc ccagccacag cacccgcaag      420

cugcagaccc gcagccagac cuggcuggag agccgcgagu acaccaagca ccugauccgc      480

guggagaacu ggaucuuccg caaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccccgcgcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggccg ccugagcagc ggccaccuga agugccgccu gaagauggac     1500

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caaguaagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  281
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 
       optimized

<400>  281
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccccgcgcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggccg ccugagcagc ggccaccuga agugccgccu gaagauggac      960

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  282
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein273-719) mRNA Design 1 
       optimized

<400>  282
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccccgcgcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagccccg caccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggccg ccugagcagc ggccaccuga agugccgccu gaagauggac      960

aagcugcgcc ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcaccgcag cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc gcauggccgu gcugggcgac accgccuggg acuucggcag cuaaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  283
<211>  10477
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein mRNA Design 1 optimized

<400>  283
gggagaaagc uuaccaugaa gaaccccaag gaggagaucc gccgcauccg caucgugaac       60

augcugaagc gcggcguggc ccgcgugaac ccccugggcg gccugaagcg ccugcccgcc      120

ggccugcugc ugggccacgg ccccauccgc auggugcugg ccauccuggc cuuccugcgc      180

uucaccgcca ucaagcccag ccugggccug aucaaccgcu ggggcagcgu gggcaagaag      240

gaggccaugg agaucaucaa gaaguucaag aaggaccugg ccgccaugcu gcgcaucauc      300

aacgcccgca aggagcgcaa gcgccgcggc gccgacacca gcaucggcau caucggccug      360

cugcugacca ccgccauggc cgccgagauc acccgccgcg gcagcgccua cuacauguac      420

cuggaccgca gcgacgccgg caaggccauc agcuucgcca ccacccuggg cgugaacaag      480

ugccacgugc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      540

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      600

accugggugg uguacggcac cugccaccac aagaagggcg aggcccgccg cagccgccgc      660

gccgugaccc ugcccagcca cagcacccgc aagcugcaga cccgcagcca gaccuggcug      720

gagagccgcg aguacaccaa gcaccugauc aagguggaga acuggaucuu ccgcaacccc      780

ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg gcagcagcac cagccagaag      840

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcauccg cugcaucggc      900

gugagcaacc gcgacuucgu ggagggcaug agcggcggca ccugggugga cguggugcug      960

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug     1020

gugaccacca ccgugagcaa cauggccgag gugcgcagcu acugcuacga ggccagcauc     1080

agcgacaugg ccagcgacag ccgcugcccc acccagggcg aggccuaccu ggacaagcag     1140

agcgacaccc aguacgugug caagcgcacc cugguggacc gcggcugggg caacggcugc     1200

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucaccugcag caagaagaug     1260

accggcaaga gcauccagcc cgagaaccug gaguaccgca ucaugcugag cgugcacggc     1320

agccagcaca gcggcaugau cggcuacgag accgacgagg accgcgccaa gguggaggug     1380

acccccaaca gcccccgcgc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1440

ugcgagcccc gcaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1500

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1560

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1620

gccaagcgcc agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1680

gccggcgccc uggaggccga gauggacggc gccaagggcc gccuguucag cggccaccug     1740

aagugccgcc ugaagaugga caagcugcgc cugaagggcg ugagcuacag ccugugcacc     1800

gccgccuuca ccuucaccaa ggugcccgcc gagacccugc acggcaccgu gaccguggag     1860

gugcaguacg ccggcaccga cggccccugc aagauccccg ugcagauggc cguggacaug     1920

cagacccuga cccccguggg ccgccugauc accgccaacc ccgugaucac cgagagcacc     1980

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     2040

ggcgugggcg acaagaagau cacccaccac uggcaccgca gcggcagcac caucggcaag     2100

gccuucgagg ccaccgugcg cggcgccaag cgcauggccg ugcugggcga caccgccugg     2160

gacuucggca gcgugggcgg cguguucaac agccugggca agggcaucca ccagaucuuc     2220

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     2280

acccugcugg uguggcuggg ccugaacacc aagaacggca gcaucagccu gaccugccug     2340

gcccugggcg gcgugaugau cuuccugagc accgccguga gcgccgacgu gggcugcagc     2400

guggacuuca gcaagaagga gacccgcugc ggcaccggcg uguucaucua caacgacgug     2460

gaggccuggc gcgaccgcua caaguaccac cccgacagcc cccgccgccu ggccgccgcc     2520

gugaagcagg ccugggagga gggcaucugc ggcaucagca gcgugagccg cauggagaac     2580

aucaugugga agagcgugga gggcgagcug aacgccaucc uggaggagaa cggcgugcag     2640

cugaccgugg uggugggcag cgugaagaac cccauguggc gcggccccca gcgccugccc     2700

gugcccguga acgagcugcc ccacggcugg aaggccuggg gcaagagcua cuucgugcgc     2760

gccgccaaga ccaacaacag cuucguggug gacggcgaca cccugaagga gugcccccug     2820

gagcaccgcg ccuggaacag cuuccuggug gaggaccacg gcuucggcgu guuccacacc     2880

agcguguggc ugaaggugcg cgaggacuac agccuggagu gcgaccccgc cgugaucggc     2940

accgccguga agggccgcga ggccgcccac agcgaccugg gcuacuggau cgagagcgag     3000

aagaacgaca ccuggcgccu gaagcgcgcc caccugaucg agaugaagac cugcgagugg     3060

cccaagagcc acacccugug gaccgacggc guggaggaga gcgaccugau cauccccaag     3120

agccuggccg gcccccugag ccaccacaac acccgcgagg gcuaccgcac ccaggugaag     3180

ggccccuggc acagcgagga gcuggagauc cgcuucgagg agugccccgg caccaaggug     3240

uacguggagg agaccugcgg cacccgcggc cccagccugc gcagcaccac cgccagcggc     3300

cgcgugaucg aggaguggug cugccgcgag ugcaccaugc ccccccugag cuuccgcgcc     3360

aaggacggcu gcugguacgg cauggagauc cgcccccgca aggagcccga gagcaaccug     3420

gugcgcagca uggugaccgc cggcagcacc gaccacaugg accacuucag ccugggcgug     3480

cuggugaucc ugcugauggu gcaggagggc cugaagaagc gcaugaccac caagaucauc     3540

augagcacca gcauggccgu gcugguggug augauccugg gcggcuucag caugagcgac     3600

cuggccaagc uggugauccu gaugggcgcc accuucgccg agaugaacac cggcggcgac     3660

guggcccacc uggcccuggu ggccgccuuc aaggugcgcc ccgcccugcu ggugagcuuc     3720

aucuuccgcg ccaacuggac cccccgcgag agcaugcugc uggcccuggc cagcugccug     3780

cugcagaccg ccaucagcgc ccuggagggc gaccugaugg ugcugaucaa cggcuucgcc     3840

cuggccuggc uggccauccg cgccauggcc gugccccgca ccgacaacau cgcccugccc     3900

auccuggccg cccugacccc ccuggcccgc ggcacccugc ugguggccug gcgcgccggc     3960

cuggccaccu gcggcggcau caugcugcug agccugaagg gcaagggcag cgugaagaag     4020

aaccugcccu ucgugauggc ccugggccug accgccgugc gcguggugga ccccaucaac     4080

guggugggcc ugcugcugcu gacccgcagc ggcaagcgca gcuggccccc cagcgaggug     4140

cugaccgccg ugggccugau cugcgcccug gccggcggcu ucgccaaggc cgacaucgag     4200

auggccggcc ccauggccgc cgugggccug cugaucguga gcuacguggu gagcggcaag     4260

agcguggaca uguacaucga gcgcgccggc gacaucaccu gggagaagga cgccgaggug     4320

accggcaaca gcccccgccu ggacguggcc cuggacgaga gcggcgacuu cagccuggug     4380

gaggaggacg gcccccccau gcgcgagauc auccugaagg uggugcugau ggccaucugc     4440

ggcaugaacc ccaucgccau ccccuucgcc gccggcgccu gguacgugua cgugaagacc     4500

ggcaagcgca gcggcgcccu gugggacgug cccgccccca aggaggugaa gaagggcgag     4560

accaccgacg gcguguaccg cgugaugacc cgccgccugc ugggcagcac ccaggugggc     4620

gugggcguga ugcaggaggg cguguuccac accauguggc acgugaccaa gggcgccgcc     4680

cugcgcagcg gcgagggccg ccuggacccc uacuggggcg acgugaagca ggaccuggug     4740

agcuacugcg gccccuggaa gcuggacgcc gccugggacg gccugagcga ggugcagcug     4800

cuggccgugc cccccggcga gcgcgcccgc aacauccaga cccugcccgg caucuucaag     4860

accaaggacg gcgacaucgg cgccguggcc cuggacuacc ccgccggcac cagcggcagc     4920

cccauccugg acaagugcgg ccgcgugauc ggccuguacg gcaacggcgu ggugaucaag     4980

aacggcagcu acgugagcgc caucacccag ggcaagcgcg aggaggagac ccccguggag     5040

ugcuucgagc ccagcaugcu gaagaagaag cagcugaccg ugcuggaccu gcaccccggc     5100

gccggcaaga cccgccgcgu gcugcccgag aucgugcgcg aggccaucaa gaagcgccug     5160

cgcaccguga uccuggcccc cacccgcgug guggccgccg agauggagga ggcccugcgc     5220

ggccugcccg ugcgcuacau gaccaccgcc gugaacguga cccacagcgg caccgagauc     5280

guggaccuga ugugccacgc caccuucacc agccgccugc ugcagcccau ccgcgugccc     5340

aacuacaacc ugaacaucau ggacgaggcc cacuucaccg accccagcag caucgccgcc     5400

cgcggcuaca ucagcacccg cguggagaug ggcgaggccg ccgccaucuu caugaccgcc     5460

accccccccg gcacccgcga cgccuucccc gacagcaaca gccccaucau ggacaccgag     5520

guggaggugc ccgagcgcgc cuggagcagc ggcuucgacu gggugaccga ccacagcggc     5580

aagaccgugu gguucgugcc cagcgugcgc aacggcaacg agaucgccgc cugccugacc     5640

aaggccggca agcgcgugau ccagcugagc cgcaagaccu ucgagaccga guuccagaag     5700

accaagaacc aggaguggga cuucgugauc accaccgaca ucagcgagau gggcgccaac     5760

uucaaggccg accgcgugau cgacagccgc cgcugccuga agcccgugau ccuggacggc     5820

gagcgcguga uccuggccgg ccccaugccc gugacccacg ccagcgccgc ccagcgccgc     5880

ggccgcaucg gccgcaaccc caacaagccc ggcgacgagu acauguacgg cggcggcugc     5940

gccgagaccg acgagggcca cgcccacugg cuggaggccc gcaugcugcu ggacaacauc     6000

uaccugcagg acggccugau cgccagccug uaccgccccg aggccgacaa gguggccgcc     6060

aucgagggcg aguucaagcu gcgcaccgag cagcgcaaga ccuucgugga gcugaugaag     6120

cgcggcgacc ugcccgugug gcuggccuac cagguggcca gcgccggcau caccuacacc     6180

gaccgccgcu ggugcuucga cggcaccacc aacaacacca ucauggagga cagcgugccc     6240

gccgaggugu ggaccaagua cggcgagaag cgcgugcuga agccccgcug gauggacgcc     6300

cgcgugugca gcgaccacgc cgcccugaag agcuucaagg aguucgccgc cggcaagcgc     6360

ggcgccgccc ugggcgugau ggaggcccug ggcacccugc ccggccacau gaccgagcgc     6420

uuccaggagg ccaucgacaa ccuggccgug cugaugcgcg ccgagaccgg cagccgcccc     6480

uacaaggccg ccgccgccca gcugcccgag acccuggaga ccaucaugcu gcugggccug     6540

cugggcaccg ugagccuggg caucuucuuc gugcugaugc gcaacaaggg caucggcaag     6600

augggcuucg gcauggugac ccugggcgcc agcgccuggc ugauguggcu gagcgagauc     6660

gagcccgccc gcaucgccug cgugcugauc gugguguucc ugcugcuggu ggugcugauc     6720

cccgagcccg agaagcagcg cagcccccag gacaaccaga uggccaucau caucauggug     6780

gccgugggcc ugcugggccu gaucaccgcc aacgagcugg gcuggcugga gcgcaccaag     6840

aacgacaucg cccaccugau gggccgccgc gaggagggcg ccaccauggg cuucagcaug     6900

gacaucgacc ugcgccccgc cagcgccugg gccaucuacg ccgcccugac cacccugauc     6960

acccccgccg ugcagcacgc cgugaccacc agcuacaaca acuacagccu gauggccaug     7020

gccacccagg ccggcgugcu guucggcaug ggcaagggca ugcccuucau gcacggcgac     7080

cugggcgugc cccugcugau gaugggcugc uacagccagc ugaccccccu gacccugauc     7140

guggccauca uccugcuggu ggcccacuac auguaccuga uccccggccu gcaggccgcc     7200

gccgcccgcg ccgcccagaa gcgcaccgcc gccggcauca ugaagaaccc cgugguggac     7260

ggcaucgugg ugaccgacau cgacaccaug accaucgacc cccaggugga gaagaagaug     7320

ggccaggugc ugcugaucgc cguggccauc agcagcgccg ugcugcugcg caccgccugg     7380

ggcuggggcg aggccggcgc ccugaucacc gccgccacca gcacccugug ggagggcagc     7440

cccaacaagu acuggaacag cagcaccgcc accagccugu gcaacaucuu ccgcggcagc     7500

uaccuggccg gcgccagccu gaucuacacc gugacccgca acgccggccu ggugaagcgc     7560

cgcggcggcg gcaccggcga gacccugggc gagaagugga aggcccgccu gaaccagaug     7620

agcgcccugg aguucuacag cuacaagaag agcggcauca ccgaggugug ccgcgaggag     7680

gcccgccgcg cccugaagga cggcguggcc accggcggcc acgccgugag ccgcggcagc     7740

gccaagaucc gcuggcugga ggagcgcggc uaccugcagc ccuacggcaa ggugguggac     7800

cugggcugcg gccgcggcgg cuggagcuac uacgccgcca ccauccgcaa ggugcaggag     7860

gugcgcggcu acaccaaggg cggccccggc cacgaggagc ccaugcuggu gcagagcuac     7920

ggcuggaaca ucgugcgccu gaagagcggc guggacgugu uccacauggc cgccgagccc     7980

ugcgacaccc ugcugugcga caucggcgag agcagcagca gccccgaggu ggaggagacc     8040

cgcacccugc gcgugcugag cauggugggc gacuggcugg agaagcgccc cggcgccuuc     8100

ugcaucaagg ugcugugccc cuacaccagc accaugaugg agaccaugga gcgccugcag     8160

cgccgccacg gcggcggccu ggugcgcgug ccccugugcc gcaacagcac ccacgagaug     8220

uacuggguga gcggcgccaa gagcaacauc aucaagagcg ugagcaccac cagccagcug     8280

cugcugggcc gcauggacgg cccccgccgc cccgugaagu acgaggagga cgugaaccug     8340

ggcagcggca cccgcgccgu ggccagcugc gccgaggccc ccaacaugaa gaucaucggc     8400

cgccgcaucg agcgcauccg caacgagcac gccgagaccu gguuccugga cgagaaccac     8460

cccuaccgca ccugggccua ccacggcagc uacgaggccc ccacccaggg cagcgccagc     8520

agccugguga acggcguggu gcgccugcug agcaagcccu gggacguggu gaccggcgug     8580

accggcaucg ccaugaccga caccaccccc uacggccagc agcgcguguu caaggagaag     8640

guggacaccc gcgugcccga cccccaggag ggcacccgcc aggugaugaa caucgugagc     8700

agcuggcugu ggaaggagcu gggcaagcgc aagcgccccc gcgugugcac caaggaggag     8760

uucaucaaca aggugcgcag caacgccgcc cugggcgcca ucuucgagga ggagaaggag     8820

uggaagaccg ccguggaggc cgugaacgac ccccgcuucu gggcccuggu ggaccgcgag     8880

cgcgagcacc accugcgcgg cgagugccac agcugcgugu acaacaugau gggcaagcgc     8940

gagaagaagc agggcgaguu cggcaaggcc aagggcagcc gcgccaucug guacaugugg     9000

cugggcgccc gcuuccugga guucgaggcc cugggcuucc ugaacgagga ccacuggaug     9060

ggccgcgaga acagcggcgg cggcguggag ggccugggcc ugcagcgccu gggcuacauc     9120

cuggaggaga ugaaccgcgc ccccggcggc aagauguacg ccgacgacac cgccggcugg     9180

gacacccgca ucagcaaguu cgaccuggag aacgaggccc ugaucaccaa ccagauggag     9240

gagggccacc gcacccuggc ccuggccgug aucaaguaca ccuaccagaa caagguggug     9300

aaggugcugc gccccgccga gggcggcaag accgugaugg acaucaucag ccgccaggac     9360

cagcgcggca gcggccaggu ggugaccuac gcccugaaca ccuucaccaa ccugguggug     9420

cagcugaucc gcaacaugga ggccgaggag gugcuggaga ugcaggaccu guggcugcug     9480

cgcaagcccg agaaggugac ccgcuggcug cagagcaacg gcugggaccg ccugaagcgc     9540

auggccguga gcggcgacga cugcguggug aagcccaucg acgaccgcuu cgcccacgcc     9600

cugcgcuucc ugaacgacau gggcaaggug cgcaaggaca cccaggagug gaagcccagc     9660

accggcugga gcaacuggga ggaggugccc uucugcagcc accacuucaa caagcuguac     9720

cugaaggacg gccgcagcau cguggugccc ugccgccacc aggacgagcu gaucggccgc     9780

gcccgcguga gccccggcgc cggcuggagc auccgcgaga ccgccugccu ggccaagagc     9840

uacgcccaga uguggcagcu gcuguacuuc caccgccgcg accugcgccu gauggccaac     9900

gccaucugca gcgccgugcc cguggacugg gugcccaccg gccgcaccac cuggagcauc     9960

cacggcaagg gcgaguggau gaccaccgag gacaugcuga ugguguggaa ccgcgugugg    10020

aucgaggaga acgaccacau ggaggacaag acccccguga ccaaguggac cgacaucccc    10080

uaccugggca agcgcgagga ccuguggugc ggcagccuga ucggccaccg cccccgcacc    10140

accugggccg agaacaucaa ggacaccgug aacauggugc gccgcaucau cggcgacgag    10200

gagaaguaca uggacuaccu gagcacccag gugcgcuacc ugggcgagga gggcagcacc    10260

cccggcgugc uguaaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc    10320

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc    10440

ccccccccca aaggcucuuu ucagagccac cagaauu                             10477


<210>  284
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 optimized

<400>  284
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

cgccgcggca gcgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcag ccgccgcgcc gugacccugc ccagccacag cacccgcaag      420

cugcagaccc gcagccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      480

guggagaacu ggaucuuccg caaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1080

gacgaggacc gcgccaaggu ggaggugacc cccaacagcc cccgcgccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggccgcc uguucagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

caccgcagcg gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  285
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 optimized

<400>  285
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

cgccgcggca gcgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcag ccgccgcgcc gugacccugc ccagccacag cacccgcaag      420

cugcagaccc gcagccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      480

guggagaacu ggaucuuccg caaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1080

gacgaggacc gcgccaaggu ggaggugacc cccaacagcc cccgcgccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggccgcc uguucagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

caccgcagcg gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca aguaaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  286
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 optimized

<400>  286
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      540

gacgaggacc gcgccaaggu ggaggugacc cccaacagcc cccgcgccga ggccacccug      600

ggcggcuucg gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc      840

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      900

aagggccgcc uguucagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug      960

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc     1140

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

caccgcagcg gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc     1320

auggccgugc ugggcgacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  287
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 optimized

<400>  287
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcauccgcug caucggcgug agcaaccgcg acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagccg cugccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

guggaccgcg gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uaccgcauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      540

gacgaggacc gcgccaaggu ggaggugacc cccaacagcc cccgcgccga ggccacccug      600

ggcggcuucg gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc      840

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      900

aagggccgcc uguucagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug      960

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc     1140

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

caccgcagcg gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc     1320

auggccgugc ugggcgacac cgccugggac uucggcagcu aaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  288
<211>  10654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein mRNA Design 2 wild
       type

<400>  288
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

acccaaaaaa gaaauccgga ggauuccgga uugucaauau gcuaaaacgc ggaguagccc      120

gugugagccc cuuugggggc uugaagaggc ugccagccgg acuucugcug ggucaugggc      180

ccaucaggau ggucuuggca auucuagccu uuuugagauu cacggcaauc aagccaucac      240

ugggucucau caauagaugg gguucagugg ggaaaaaaga ggcuauggaa auaauaaaga      300

aguucaagaa agaucuggcu gccaugcuga gaauaaucaa ugcuaggaag gagaagaaga      360

gacggggcgc agauacuagu gucggaauug uuggccuccu gcugaccaca gcuauggcag      420

cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      480

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      540

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      600

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      660

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      720

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      780

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      840

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      900

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      960

aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu ugugucaccg     1020

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca     1080

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc     1140

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca     1200

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggaguc     1260

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1320

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1380

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1440

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1500

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1560

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1620

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1680

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1740

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1800

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1860

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1920

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1980

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     2040

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     2100

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     2160

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     2220

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu ggagcagcuu     2280

ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga acguugcuga     2340

ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2400

gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcucg guggacuucu     2460

caaagaagga gacgagaugc gguacagggg uguucgucua uaacgacguu gaagccugga     2520

gggacaggua caaguaccau ccugacuccc cccguagauu ggcagcagca gucaagcaag     2580

ccugggaaga ugguaucugc gggaucuccu cuguuucaag aauggaaaac aucaugugga     2640

gaucaguaga aggggagcuc aacgcaaucc uggaagagaa uggaguucaa cugacggucg     2700

uugugggauc uguaaaaaac cccaugugga gagguccaca gagauugccc gugccuguga     2760

acgagcugcc ccacggcugg aaggcuuggg ggaaaucgca cuucgucaga gcagcaaaga     2820

caaauaacag cuuugucgug gauggugaca cacugaagga augcccacuc aaacauagag     2880

cauggaacag cuuucuugug gaggaucaug gguucggggu auuucacacu agugucuggc     2940

ucaagguuag agaagauuau ucauuagagu gugauccagc cguuauugga acagcuguua     3000

agggaaagga ggcuguacac agugaucuag gcuacuggau ugagagugag aagaaugaca     3060

cauggaggcu gaagagggcc caucugaucg agaugaaaac augugaaugg ccaaaguccc     3120

acacauugug gacagaugga auagaagaga gugaucugau cauacccaag ucuuuagcug     3180

ggccacucag ccaucacaau accagagagg gcuacaggac ccaaaugaaa gggccauggc     3240

acagugaaga gcuugaaauu cgguuugagg aaugcccagg cacuaagguc cacguggagg     3300

aaacaugugg aacaagagga ccaucucuga gaucaaccac ugcaagcgga agggugaucg     3360

aggaauggug cugcagggag ugcacaaugc ccccacuguc guuccgggcu aaagauggcu     3420

guugguaugg aauggagaua aggcccagga aagaaccaga aagcaacuua guaaggucaa     3480

uggugacugc aggaucaacu gaucacaugg aucacuucuc ccuuggagug cuugugauuc     3540

ugcucauggu gcaggaaggg cugaagaaga gaaugaccac aaagaucauc auaagcacau     3600

caauggcagu gcugguagcu augauccugg gaggauuuuc aaugagugac cuggcuaagc     3660

uugcaauuuu gaugggugcc accuucgcgg aaaugaacac uggaggagau guagcucauc     3720

uggcgcugau agcggcauuc aaagucagac cagcguugcu gguaucuuuc aucuucagag     3780

cuaauuggac accccgugaa agcaugcugc uggccuuggc cucgugucuu uugcaaacug     3840

cgaucuccgc cuuggaaggc gaccugaugg uucucaucaa ugguuuugcu uuggccuggu     3900

uggcaauacg agcgaugguu guuccacgca cugauaacau caccuuggca auccuggcug     3960

cucugacacc acuggcccgg ggcacacugc uuguggcgug gagagcaggc cuugcuacuu     4020

gcgggggguu uaugcuccuc ucucugaagg gaaaaggcag ugugaagaag aacuuaccau     4080

uugucauggc ccugggacua accgcuguga ggcuggucga ccccaucaac gugguggggc     4140

ugcuguugcu cacaaggagu gggaagcgga gcuggccccc uagcgaagua cucacagcug     4200

uuggccugau augcgcauug gcuggagggu ucgccaaggc agauauagag auggcugggc     4260

ccauggccgc ggucggucug cuaauuguca guuacguggu cucaggaaag aguguggaca     4320

uguacauuga aagagcaggu gacaucacau gggaaaaaga ugcggaaguc acuggaaaca     4380

guccccggcu cgauguggcg cuagaugaga guggugauuu cucccuggug gaggaugacg     4440

guccccccau gagagagauc auacucaagg ugguccugau gaccaucugu ggcaugaacc     4500

caauagccau acccuuugca gcuggagcgu gguacguaua cgugaagacu ggaaaaagga     4560

guggugcucu augggaugug ccugcuccca aggaaguaaa aaagggggag accacagaug     4620

gaguguacag aguaaugacu cguagacugc uagguucaac acaaguugga gugggaguua     4680

ugcaagaggg ggucuuucac acuauguggc acgucacaaa aggauccgcg cugagaagcg     4740

gugaagggag acuugaucca uacuggggag augucaagca ggaucuggug ucauacugug     4800

guccauggaa gcuagaugcc gccugggacg ggcacagcga ggugcagcuc uuggccgugc     4860

cccccggaga gagagcgagg aacauccaga cucugcccgg aauauuuaag acaaaggaug     4920

gggacauugg agcgguugcg cuggauuacc cagcaggaac uucaggaucu ccaauccuag     4980

acaagugugg gagagugaua ggacuuuaug gcaauggggu cgugaucaaa aaugggaguu     5040

auguuagugc caucacccaa gggaggaggg aggaagagac uccuguugag ugcuucgagc     5100

cuucgaugcu gaagaagaag cagcuaacug ucuuagacuu gcauccugga gcugggaaaa     5160

ccaggagagu ucuuccugaa auaguccgug aagccauaaa aacaagacuc cguacuguga     5220

ucuuagcucc aaccaggguu gucgcugcug aaauggagga agcccuuaga gggcuuccag     5280

ugcguuauau gacaacagca gucaauguca cccacucugg aacagaaauc gucgacuuaa     5340

ugugccaugc caccuucacu ucacgucuac uacagccaau cagagucccc aacuauaauc     5400

uguauauuau ggaugaggcc cacuucacag aucccucaag uauagcagca agaggauaca     5460

uuucaacaag gguugagaug ggcgaggcgg cugccaucuu caugaccgcc acgccaccag     5520

gaacccguga cgcauuuccg gacuccaacu caccaauuau ggacaccgaa guggaagucc     5580

cagagagagc cuggagcuca ggcuuugauu gggugacgga uuauucugga aaaacaguuu     5640

gguuuguucc aagcgugagg aacggcaaug agaucgcagc uugucugaca aaggcuggaa     5700

aacgggucau acagcucagc agaaagacuu uugagacaga guuccagaaa acaaaacauc     5760

aagaguggga cuuugucgug acaacugaca uuucagagau gggcgccaac uuuaaagcug     5820

accgugucau agauuccagg agaugccuaa agccggucau acuugauggc gagagaguca     5880

uucuggcugg acccaugccu gucacacaug ccagcgcugc ccagaggagg gggcgcauag     5940

gcaggaaucc caacaaaccu ggagaugagu aucuguaugg aggugggugc gcagagacug     6000

acgaagacca ugcacacugg cuugaagcaa gaaugcuccu ugacaauauu uaccuccaag     6060

auggccucau agccucgcuc uaucgaccug aggccgacaa aguagcagcc auugagggag     6120

aguucaagcu uaggacggag caaaggaaga ccuuugugga acucaugaaa agaggagauc     6180

uuccuguuug gcuggccuau cagguugcau cugccggaau aaccuacaca gauagaagau     6240

ggugcuuuga uggcacgacc aacaacacca uaauggaaga cagugugccg gcagaggugu     6300

ggaccagaca cggagagaaa agagugcuca aaccgaggug gauggacgcc agaguuuguu     6360

cagaucaugc ggcccugaag ucauucaagg aguuugccgc ugggaaaaga ggagcggcuu     6420

uuggagugau ggaagcccug ggaacacugc caggacacau gacagagaga uuccaggaag     6480

ccauugacaa ccucgcugug cucaugcggg cagagacugg aagcaggccu uacaaagccg     6540

cggcggccca auugccggag acccuagaga ccauuaugcu uuugggguug cugggaacag     6600

ucucgcuggg aaucuuuuuc gucuugauga ggaacaaggg cauagggaag augggcuuug     6660

gaauggugac ucuuggggcc agcgcauggc ucauguggcu cucggaaauu gagccagcca     6720

gaauugcaug uguccucauu guuguguucc uauugcuggu ggugcucaua ccugagccag     6780

aaaagcaaag aucuccccag gacaaccaaa uggcaaucau caucauggua gcaguagguc     6840

uucugggcuu gauuaccgcc aaugaacucg gaugguugga gagaacaaag agugaccuaa     6900

gccaucuaau gggaaggaga gaggaggggg caaccauggg auucucaaug gacauugacc     6960

ugcggccagc cucagcuugg gccaucuaug cugccuugac aacuuucauu accccagccg     7020

uccaacaugc agugaccacu ucauacaaca acuacuccuu aauggcgaug gccacgcaag     7080

cuggaguguu guuugguaug ggcaaaggga ugccauucua cgcaugggac uuuggagucc     7140

cgcugcuaau gauagguugc uacucacaau uaacgccccu gacccuaaua guggccauca     7200

uuuugcucgu ggcgcacuac auguacuuga ucccagggcu gcaggcagca gcugcgcgug     7260

cugcccagaa gagaacggca gcuggcauca ugaagaaccc uguuguggau ggaauagugg     7320

ugacugacau ugacacaaug acaauugacc cccaagugga gaaaaagaug ggacaggugc     7380

uacucauggc aguagccguc uccagcgcca uacugucgcg gaccgccugg gggugggggg     7440

aggcuggggc ccugaucaca gccgcaacuu ccacuuugug ggaaggcucu ccgaacaagu     7500

acuggaacuc cucuacagcc acuucacugu guaacauuuu uaggggaagu uacuuggcug     7560

gagcuucucu aaucuacaca guaacaagaa acgcuggcuu ggucaagaga cgugggggug     7620

gaacaggaga gacccuggga gagaaaugga aggcccgcuu gaaccagaug ucggcccugg     7680

aguucuacuc cuacaaaaag ucaggcauca ccgaggugug cagagaagag gcccgccgcg     7740

cccucaagga cgguguggca acgggaggcc augcuguguc ccgaggaagu gcaaagcuga     7800

gaugguuggu ggagcgggga uaccugcagc ccuauggaaa ggucauugau cuuggaugug     7860

gcagaggggg cuggaguuac uacgccgcca ccauccgcaa aguucaagaa gugaaaggau     7920

acacaaaagg aggcccuggu caugaagaac ccguguuggu gcaaagcuau ggguggaaca     7980

uaguccgucu uaagaguggg guggacgucu uucauauggc ggcugagccg ugugacacgu     8040

ugcuguguga cauaggugag ucaucaucua guccugaagu ggaagaagca cggacgcuca     8100

gaguccucuc cauggugggg gauuggcuug aaaaaagacc aggagccuuu uguauaaaag     8160

uguugugccc auacaccagc acuaugaugg aaacccugga gcgacugcag cguagguaug     8220

ggggaggacu ggucagagug ccacucuccc gcaacucuac acaugagaug uacugggucu     8280

cuggagcgaa aagcaacacc auaaaaagug uguccaccac gagccagcuc cucuuggggc     8340

gcauggacgg gccuaggagg ccagugaaau augaggagga ugugaaucuc ggcucuggca     8400

cgcgggcugu gguaagcugc gcugaagcuc ccaacaugaa gaucauuggu aaccgcauug     8460

aaaggauccg cagugagcac gcggaaacgu gguucuuuga cgagaaccac ccauauagga     8520

caugggcuua ccauggaagc uaugaggccc ccacacaagg gucagcgucc ucucuaauaa     8580

acgggguugu caggcuccug ucaaaacccu gggauguggu gacuggaguc acaggaauag     8640

ccaugaccga caccacaccg uauggucagc aaagaguuuu caaggaaaaa guggacacua     8700

gggugccaga cccccaagaa gguacucguc agguuaugag cauggucucu uccugguugu     8760

ggaaagagcu aggcaaacac aaacggccac gagucuguac caaagaagag uucaucaaca     8820

agguucguag caaugcagca uuaggggcaa uauuugaaga ggaaaaagag uggaagacug     8880

caguggaagc ugugaacgau ccaagguucu gggcucuagu ggacaaggaa agagagcacc     8940

accugagagg agagugccag aguugugugu acaacaugau gggaaaaaga gaaaagaaac     9000

aaggggaauu uggaaaggcc aagggcagcc gcgccaucug guauaugugg cuaggggcua     9060

gauuucuaga guucgaagcc cuuggauucu ugaacgagga ucacuggaug gggagagaga     9120

acucaggagg ugguguugaa gggcugggau uacaaagacu cggauauguc cuagaagaga     9180

ugagucgcau accaggagga aggauguaug cagaugacac ugcuggcugg gacacccgca     9240

ucagcagguu ugaucuggag aaugaagcuc uaaucaccaa ccaaauggag aaagggcaca     9300

gggccuuggc auuggccaua aucaaguaca cauaccaaaa caaaguggua aagguccuua     9360

gaccagcuga aaaagggaaa acaguuaugg acauuauuuc gagacaagac caaaggggga     9420

gcggacaagu ugucacuuac gcucuuaaca cauuuaccaa ccuaguggug caacucauuc     9480

ggaauaugga ggcugaggaa guccuagaga ugcaagacuu guggcugcug cggaggucag     9540

agaaagugac caacugguug cagagcaacg gaugggauag gcucaaacga auggcaguca     9600

guggagauga uugcguugug aagccaauug augauagguu ugcacaugcc cucagguucu     9660

ugaaugauau gggaaaaguu aggaaggaca cacaagagug gaaacccuca acuggauggg     9720

acaacuggga agaaguuccg uuuugcuccc accacuucaa caagcuccau cucaaggacg     9780

ggagguccau ugugguuccc ugccgccacc aagaugaacu gauuggccgg gcccgcgucu     9840

cuccaggggc gggauggagc auccgggaga cugcuugccu agcaaaauca uaugcgcaaa     9900

uguggcagcu ccuuuauuuc cacagaaggg accuccgacu gauggccaau gccauuuguu     9960

caucugugcc aguugacugg guuccaacug ggagaacuac cuggucaauc cauggaaagg    10020

gagaauggau gaccacugaa gacaugcuug ugguguggaa cagagugugg auugaggaga    10080

acgaccacau ggaagacaag accccaguua cgaaauggac agacauuccc uauuugggaa    10140

aaagggaaga cuuguggugu ggaucucuca uagggcacag accgcgcacc accugggcug    10200

agaacauuaa aaacacaguc aacauggugc gcaggaucau aggugaugaa gaaaaguaca    10260

uggacuaccu auccacccaa guucgcuacu ugggugaaga agggucuaca ccuggagugc    10320

uguaaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag    10380

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac    10440

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua    10500

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc    10620

ccccccaaag gcucuuuuca gagccaccag aauu                                10654


<210>  289
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 wild 
       type

<400>  289
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac ggggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  290
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4797 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2
       wild type

<400>  290
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac ggggcgcaga uaccaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  291
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 wild
       type

<400>  291
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  292
<211>  10654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein mRNA Design 2 wild 
       type

<400>  292
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

acccaaaaaa gaaauccgga ggauuccgga uugucaauau gcuaaaacgc ggaguagccc      120

gugugagccc cuuugggggc uugaagaggc ugccagccgg acuucugcug ggucaugggc      180

ccaucaggau ggucuuggcg auucuagccu uuuugagauu cacggcaauc aagccaucac      240

ugggucucau caauagaugg gguucagugg ggaaaaaaga ggcuauggaa auaauaaaga      300

aguucaagaa agaucuggcu gccaugcuga gaauaaucaa ugcuaggaag gagaagaaga      360

gacgaggcgc agauacuagu gucggaauug uuggccuccu gcugaccaca gcuauggcag      420

cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      480

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      540

uuggacacac gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      600

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      660

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      720

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      780

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      840

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      900

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      960

aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu ugugucacug     1020

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca     1080

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc     1140

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca     1200

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggagcc     1260

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1320

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1380

uuaaugacac aggacaugaa acugaugaga auagagcgaa aguugagaua acgcccaauu     1440

caccaagagc cgaagccacc cugggggggu uuggaagccu aggacuugau ugugaaccga     1500

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacuggcugg     1560

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1620

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1680

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1740

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1800

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacu gcagcguuca     1860

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1920

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1980

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     2040

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     2100

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     2160

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     2220

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaaucuuu ggagcagcuu     2280

ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga acguugcuga     2340

ugugguuggg ucugaacgca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2400

gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcucg guggacuucu     2460

caaagaagga gacgagaugc gguacagggg uguucgucua uaacgacguu gaagccugga     2520

gggacaggua caaguaccau ccugacuccc cccguagauu ggcagcagca gucaagcaag     2580

ccugggaaga ugguaucugc gggaucuccu cuguuucaag aauggaaaac aucaugugga     2640

gaucaguaga aggggagcuc aacgcaaucc uggaagagaa uggaguucaa cugacggucg     2700

uugugggauc uguaaaaaac cccaugugga gagguccaca gagauugccc gugccuguga     2760

acgagcugcc ccacggcugg aaggcuuggg ggaaaucgua cuucgucaga gcagcaaaga     2820

caaauaacag cuuugucgug gauggugaca cacugaagga augcccacuc aaacauagag     2880

cauggaacag cuuucuugug gaggaucaug gguucggggu auuucacacu agugucuggc     2940

ucaagguuag agaagauuau ucauuagagu gugauccagc cguuauugga acagcuguua     3000

agggaaagga ggcuguacac agugaucuag gcuacuggau ugagagugag aagaaugaca     3060

cauggaggcu gaagagggcc caucugaucg agaugaaaac augugaaugg ccaaaguccc     3120

acacauugug gacagaugga auagaagaga gugaucugau cauacccaag ucuuuagcug     3180

ggccacucag ccaucacaau accagagagg gcuacaggac ccaaaugaaa gggccauggc     3240

acagugaaga gcuugaaauu cgguuugagg aaugcccagg cacuaagguc cacguggagg     3300

aaacaugugg aacgagagga ccaucucuga gaucaaccac ugcaagcgga agggugaucg     3360

aggaauggug cugcagggag ugcacaaugc ccccacuguc guuccgggcu aaagauggcu     3420

guugguaugg aauggagaua aggcccagga aagaaccaga aagcaacuua guaaggucaa     3480

uggugacugc aggaucaacu gaucacaugg accacuucuc ccuuggagug cuugugauuc     3540

ugcucauggu gcaggaaggg uugaagaaga gaaugaccac aaagaucauc auaagcacau     3600

caauggcagu gcugguagcu augauccugg gaggauuuuc aaugagugac cuggcuaagc     3660

uugcaauuuu gaugggugcc accuucgcgg aaaugaacac uggaggagau guagcucauc     3720

uggcgcugau agcggcauuc aaagucagac cagcguugcu gguaucuuuc aucuucagag     3780

cuaauuggac accccgugaa agcaugcugc uggccuuggc cucgugucuu uugcaaacug     3840

cgaucuccgc cuuggaaggc gaccugaugg uucucaucaa ugguuuugcu uuggccuggu     3900

uggcaauacg agcgaugguu guuccacgca cugauaacau caccuuggca auccuggcug     3960

cucugacacc acuggcccgg ggcacacugc uuguggcgug gagagcaggc cuugcuacuu     4020

gcgggggguu uaugcuccuc ucucugaagg gaaaaggcag ugugaagaag aacuuaccau     4080

uugucauggc ccugggacua accgcuguga ggcuggucga ccccaucaac guggugggac     4140

ugcuguugcu cacaaggagu gggaagcgga gcuggccccc uagcgaagua cucacagcug     4200

uuggccugau augcgcauug gcuggagggu ucgccaaggc agauauagag auggcugggc     4260

ccauggccgc ggucggucug cuaauuguca guuacguggu cucaggaaag aguguggaca     4320

uguacauuga aagagcaggu gacaucacau gggaaaaaga ugcggaaguc acuggaaaca     4380

guccccggcu cgauguggcg cuagaugaga guggugauuu cucccuggug gaggaugacg     4440

guccccccau gagagagauc auacucaagg ugguccugau gaccaucugu ggcaugaacc     4500

caauagccau acccuuugca gcuggagcgu gguacguaua cgugaagacu ggaaaaagga     4560

guggugcucu augggaugug ccugcuccca aggaaguaaa aaagggggag accacagaug     4620

gaguguacag aguaaugacu cguagacugc uagguucaac acaaguugga gugggaguua     4680

ugcaagaggg ggucuuucac acuauguggc acgucacaaa aggauccgcg cugagaagcg     4740

gugaagggag acuugaucca uacuggggag augucaagca ggaucuggug ucauacugug     4800

guccauggaa gcuagaugcc gccugggacg ggcacagcga ggugcagcuc uuggccgugc     4860

cccccggaga gagagcgagg aacauccaga cucugcccgg aauauuuaag acaaaggaug     4920

gggacauugg agcgguugcg cuggauuacc cagcaggaac uucaggaucu ccuauccuag     4980

acaagugugg gagagugaua ggacuuuaug gcaauggggu cgugaucaaa aaugggaguu     5040

auguuagugc caucacccaa gggaggaggg aggaagagac uccuguugag ugcuucgagc     5100

cuucgaugcu gaagaagaag cagcuaacug ucuuagacuu gcauccugga gcugggaaaa     5160

ccaggagagu ucuuccugaa auaguccgug aagccauaaa aacaagacuc cguacuguga     5220

ucuuagcucc aaccaggguu gucgcugcug aaauggagga ggcccuuaga gggcuuccag     5280

ugcguuauau gacaacagca gucaauguca cccacucugg aacagaaauc gucgacuuaa     5340

ugugccaugc caccuucacu ucgcgucuac uacagccaau cagagucccc aacuauaauc     5400

uguauauuau ggaugaggcc cacuucacag aucccucaag uauagcagca agaggauaca     5460

uuucaacaag gguugagaug ggcgaggcgg ccgccaucuu caugaccgcc acgccaccag     5520

gaacccguga cgcauuuccg gacuccaacu caccaauuau ggacaccgaa guggaagucc     5580

cagagagagc cuggagcuca ggcuuugauu gggugacgga ucauucugga aaaacaguuu     5640

gguuuguucc aagcgugagg aacggcaaug agaucgcagc uugucugaca aaggcuggaa     5700

aacgggucau acagcucagc agaaagacuu uugagacaga guuccagaaa acaaaacauc     5760

aagaguggga cuuugucgug acaacugaca uuucagagau gggcgccaac uuuaaagcug     5820

accgugucau agauuccagg agaugccuaa agccggucau acuugauggc gagagaguca     5880

uucuggcugg acccaugccu gucacacaug ccagcgcugc ccagaggagg gggcgcauag     5940

gcaggaaucc caacaaaccu ggagaugagu aucuguaugg aggugggugc gcagagacug     6000

acgaagacca ugcacacugg cuugaagcaa gaaugcuccu ugacaauauu uaccuccaag     6060

auggccucau agccucgcuc uaucgaccug aggccgacaa aguagcagcc auugagggag     6120

aguucaagcu uaggacggag caaaggaaga ccuuugugga acucaugaaa agaggagauc     6180

uuccuguuug gcuggccuau cagguugcau cugccggaau aaccuacaca gauagaagau     6240

ggugcuuuga uggcacgacc aacaacacca uaauggaaga cagugugccg gcagaagugu     6300

ggaccagaca cggagagaaa agagugcuca aaccgaggug gauggacgcc agaguuuguu     6360

cagaucaugc ggcccugaag ucauucaagg aguuugccgc ugggaaaaga ggagcggcuu     6420

uuggagugau ggaagcccug ggaacacugc caggacacau gacagagaga uuccaggaag     6480

ccauugacaa ccucgcugug cucaugcggg cagagacugg aagcaggccu uacaaagccg     6540

cggcggccca auugccggag acccuagaga ccauuaugcu uuugggguug cugggaacag     6600

ucucgcuggg aaucuucuuc gucuugauga ggaacaaggg cauagggaag augggcuuug     6660

gaauggugac ucuuggggcc agcgcauggc ucauguggcu cucggaaauu gagccagcca     6720

gaauugcaug uguccucauu guuguguucc uauugcuggu ggugcucaua ccugagccag     6780

aaaagcaaag aucuccccag gacaaccaaa uggcaaucau caucauggua gcaguagguc     6840

uucugggcuu gauuaccgcc aaugaacucg gaugguugga gagaacaaag agugaccuaa     6900

gccaucuaau gggaaggaga gaggaggggg caaccauagg auucucaaug gacauugacc     6960

ugcggccagc cucagcuugg gccaucuaug cugccuugac aacuuucauu accccagccg     7020

uccaacaugc agugaccacc ucauacaaca acuacuccuu aauggcgaug gccacgcaag     7080

cuggaguguu guuugguaug ggcaaaggga ugccauucua cgcaugggac uuuggagucc     7140

cgcugcuaau gauagguugc uacucacaau uaacaccccu gacccuaaua guggccauca     7200

uuuugcucgu ggcgcacuac auguacuuga ucccagggcu gcaggcagca gcugcgcgug     7260

cugcccagaa gagaacggca gcuggcauca ugaagaaccc uguuguggau ggaauagugg     7320

ugacugacau ugacacaaug acaauugacc cccaagugga gaaaaagaug ggacaggugc     7380

uacucauagc aguagccguc uccagcgcca uacugucgcg gaccgccugg gggugggggg     7440

aggcuggggc ccugaucaca gccgcaacuu ccacuuugug ggaaggcucu ccgaacaagu     7500

acuggaacuc cucuacagcc acuucacugu guaacauuuu uaggggaagu uacuuggcug     7560

gagcuucucu aaucuacaca guaacaagaa acgcuggcuu ggucaagaga cgugggggug     7620

gaacaggaga gacccuggga gagaaaugga aggcccgcuu gaaccagaug ucggcccugg     7680

aguucuacuc cuacaaaaag ucaggcauca ccgaggugug cagagaagag gcccgccgcg     7740

cccucaagga cgguguggca acgggaggcc augcuguguc ccgaggaagu gcaaagcuga     7800

gaugguuggu ggagcgggga uaccugcagc ccuauggaaa ggucauugau cuuggaugug     7860

gcagaggggg cuggaguuac uacgccgcca ccauccgcaa aguucaagaa gugaaaggau     7920

acacaaaagg aggcccuggu caugaagaac ccguguuggu gcaaagcuau ggguggaaca     7980

uaguccgucu uaagaguggg guggacgucu uucauauggc ggcugagccg ugugacacgu     8040

ugcuguguga cauaggugag ucaucaucua guccugaagu ggaagaagca cggacgcuca     8100

gaguccucuc cauggugggg gauuggcuug aaaaaagacc aggagccuuu uguauaaaag     8160

uguugugccc auacaccagc acuaugaugg aaacccugga gcgacugcag cguagguaug     8220

ggggaggacu ggucagagug ccacucuccc gcaacucuac acaugagaug uacugggucu     8280

cuggagcgaa aagcaacacc auaaaaagug uguccaccac gagccagcuc cucuuggggc     8340

gcauggacgg gccuaggagg ccagugaaau augaggagga ugugaaucuc ggcucuggca     8400

cgcgggcugu gguaagcugc gcugaagcuc ccaacaugaa gaucauuggu aaccgcauug     8460

aaaggauccg cagugagcac gcggaaacgu gguucuuuga cgagaaccac ccauauagga     8520

caugggcuua ccauggaagc uaugaggccc ccacacaagg gucagcgucc ucucuaauaa     8580

acgggguugu caggcuccug ucaaaacccu gggauguggu gacuggaguc acaggaauag     8640

ccaugaccga caccacaccg uauggucagc aaagaguuuu caaggaaaaa guggacacua     8700

gggugccaga cccccaagaa ggcacucguc agguuaugag cauggucucu uccugguugu     8760

ggaaagagcu aggcaaacac aaacggccac gagucuguac caaagaagag uucaucaaca     8820

agguucguag caaugcagca uuaggggcaa uauuugaaga ggaaaaagag uggaagacug     8880

caguggaagc ugugaacgau ccaagguucu gggcucuagu ggacaaggaa agagagcacc     8940

accugagagg agagugccag aguugugugu acaacaugau gggaaaaaga gaaaagaaac     9000

aaggggaauu uggaaaggcc aagggcagcc gcgccaucug guauaugugg cuaggggcua     9060

gauuucuaga guucgaagcc cuuggauucu ugaacgagga ucacuggaug gggagagaga     9120

acucaggagg ugguguugaa gggcugggau uacaaagacu cggauauguc cuagaagaga     9180

ugagucguau accaggagga aggauguaug cagaugacac ugcuggcugg gacacccgca     9240

uuagcagguu ugaucuggag aaugaagcuc uaaucaccaa ccaaauggag aaagggcaca     9300

gggccuuggc auuggccaua aucaaguaca cauaccaaaa caaaguggua aagguccuua     9360

gaccagcuga aaaagggaaa acaguuaugg acauuauuuc gagacaagac caaaggggga     9420

gcggacaagu ugucacuuac gcucuuaaca cauuuaccaa ccuaguggug caacucauuc     9480

ggaauaugga ggcugaggaa guucuagaga ugcaagacuu guggcugcug cggaggucag     9540

agaaagugac uaacugguug cagagcaacg gaugggauag gcucaaacga auggcaguca     9600

guggagauga uugcguugug aagccaauug augauagguu ugcacaugcc cucagguucu     9660

ugaaugauau gggaaaaguu aggaaggaca cacaagagug gaaacccuca acuggauggg     9720

acaacuggga agaaguuccg uuuugcuccc accacuucaa caagcuccau cucaaggacg     9780

ggagguccau ugugguuccc ugccgccacc aagaugaacu gauuggccgg gcccgcgucu     9840

cuccaggggc gggauggagc auccgggaga cugcuugccu agcaaaauca uaugcgcaaa     9900

uguggcagcu ccuuuauuuc cacagaaggg accuccgacu gauggccaau gccauuuguu     9960

caucugugcc aguugacugg guuccaacug ggagaacuac cuggucaauc cauggaaagg    10020

gagaauggau gaccacugaa gacaugcuug ugguguggaa cagagugugg auugaggaga    10080

acgaccacau ggaagacaag accccaguua cgaaauggac agacauuccc uauuugggaa    10140

aaagggaaga cuuguggugu ggaucucuca uagggcacag accgcgcacc accugggcug    10200

agaacauuaa aaacacaguc aacauggugc gcaggaucau aggugaugaa gaaaaguaca    10260

uggacuaccu auccacccaa guucgcuacu ugggugaaga agggucuaca ccuggagugc    10320

uguaaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag    10380

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac    10440

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua    10500

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc    10620

ccccccaaag gcucuuuuca gagccaccag aauu                                10654


<210>  293
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 wild 
       type

<400>  293
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacacgug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aaucuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacgcaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  294
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 wild 
       type

<400>  294
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacgaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacacgug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aaucuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacgcaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  295
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 wild 
       type

<400>  295
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  296
<211>  10642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein mRNA Design 2 wild type

<400>  296
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

accccaaaga agaaauccgg aggauccgga uugucaauau gcuaaaacgc ggaguagccc      120

guguaaaccc cuugggaggu uugaagaggu ugccagccgg acuucugcug ggucauggac      180

ccaucagaau gguuuuggcg auacuagccu uuuugagauu uacagcaauc aagccaucac      240

ugggccuuau caacagaugg gguuccgugg ggaaaaaaga ggcuauggaa auaauaaaga      300

aguucaagaa agaucuugcu gccauguuga gaauaaucaa ugcuaggaaa gagaggaaga      360

gacguggcgc agacaccagc aucggaauca uuggccuccu gcugacuaca gccauggcag      420

cagagaucac uagacgcggg agugcauacu acauguacuu ggauaggagc gaugccggga      480

aggccauuuc guuugcuacc acauugggag ugaacaagug ccacguacag aucauggacc      540

ucgggcacau gugugacgcc accaugaguu augagugccc uaugcuggau gagggagugg      600

aaccagauga ugucgauugc uggugcaaca cgacaucaac uuggguugug uacggaaccu      660

gucaucacaa aaaaggugag gcacggcgau cuagaagagc cgugacgcuc ccuucucacu      720

cuacaaggaa guugcaaacg cggucgcaga ccugguuaga aucaagagaa uacacgaagc      780

acuugaucaa gguugaaaac uggauauuca ggaaccccgg guuugcgcua guggccguug      840

ccauugccug gcuuuuggga agcucgacga gccaaaaagu cauauacuug gucaugauac      900

ugcugauugc cccggcauac aguaucaggu gcauuggagu cagcaauaga gacuucgugg      960

agggcauguc aggugggacc uggguugaug uugucuugga acauggaggc ugcguuaccg     1020

ugauggcaca ggacaagcca acagucgaca uagaguuggu cacgacgacg guuaguaaca     1080

uggccgaggu aagauccuau ugcuacgagg caucgauauc ggacauggcu ucggacaguc     1140

guugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca     1200

aaagaacauu aguggacaga gguuggggaa acgguugugg acuuuuuggc aaagggagcu     1260

uggugacaug ugccaaguuu acguguucua agaagaugac cgggaagagc auucaaccgg     1320

aaaaucugga guaucggaua augcuaucag ugcauggcuc ccagcauagc gggaugauug     1380

gauaugaaac ugacgaagau agagcgaaag ucgagguuac gccuaauuca ccaagagcgg     1440

aagcaaccuu gggaggcuuu ggaagcuuag gacuugacug ugaaccaagg acaggccuug     1500

acuuuucaga ucuguauuac cugaccauga acaauaagca uugguuggug cacaaagagu     1560

gguuucauga caucccauug ccuuggcaug cuggggcaga caccggaacu ccacacugga     1620

acaacaaaga ggcauuggua gaauucaagg augcccacgc caagaggcaa accgucgucg     1680

uucuggggag ccaggaagga gccguucaca cggcucucgc uggagcucua gaggcugaga     1740

uggauggugc aaagggaagg cuguucucug gccauuugaa augccgccua aaaauggaca     1800

agcuuagauu gaagggcgug ucauauuccu ugugcacugc ggcauucaca uucaccaagg     1860

ucccagcuga aacacugcau ggaacaguca caguggaggu gcaguaugca gggacagaug     1920

gacccugcaa gaucccaguc cagauggcgg uggacaugca gacccugacc ccaguuggaa     1980

ggcugauaac cgccaacccc gugauuacug aaagcacuga gaacucaaag augauguugg     2040

agcuugaccc accauuuggg gauucuuaca uugucauagg aguuggggac aagaaaauca     2100

cccaccacug gcauaggagu gguagcacca ucggaaaggc auuugaggcc acugugagag     2160

gcgccaagag aauggcaguc cugggggaua cagccuggga cuucggauca gucgggggug     2220

uguucaacuc acuggguaag ggcauucacc agauuuuugg agcagccuuc aaaucacugu     2280

uuggaggaau guccugguuc ucacagaucc ucauaggcac gcugcuagug ugguuagguu     2340

ugaacacaaa gaauggaucu aucucccuca caugcuuggc ccugggggga gugaugaucu     2400

uccucuccac ggcuguuucu gcugacgugg ggugcucagu ggacuucuca aaaaaggaaa     2460

cgagaugugg cacgggggua uucaucuaua augauguuga agccuggagg gaccgguaca     2520

aguaccaucc ugacuccccc cgcagauugg cagcagcagu caagcaggcc ugggaagagg     2580

ggaucugugg gaucucaucc guuucaagaa uggaaaacau cauguggaaa ucaguagaag     2640

gggagcucaa ugcuauccua gaggagaaug gaguucaacu gacaguuguu gugggaucug     2700

uaaaaaaccc cauguggaga gguccacaaa gauugccagu gccugugaau gagcugcccc     2760

auggcuggaa agccuggggg aaaucguauu uuguuagggc ggcaaagacc aacaacaguu     2820

uuguugucga cggugacaca cugaaggaau guccgcuuga gcacagagca uggaauaguu     2880

uucuugugga ggaucacggg uuuggagucu uccacaccag ugucuggcuu aaggucagag     2940

aagauuacuc auuagaaugu gacccagccg ucauaggaac agcuguuaag ggaagggagg     3000

ccgcgcacag ugaucugggc uauuggauug aaagugaaaa gaaugacaca uggaggcuga     3060

agagggccca ccugauugag augaaaacau gugaauggcc aaagucucac acauugugga     3120

cagauggagu agaagaaagu gaucuuauca uacccaaguc uuuagcuggu ccacucagcc     3180

accacaacac cagagagggu uacagaaccc aagugaaagg gccauggcac agugaagagc     3240

uugaaauccg guuugaggaa uguccaggca ccaagguuua cguggaggag acaugcggaa     3300

cuagaggacc aucucugaga ucaacuacug caaguggaag ggucauugag gaauggugcu     3360

guagggaaug cacaaugccc ccacuaucgu uucgagcaaa agacggcugc ugguauggaa     3420

uggagauaag gcccaggaaa gaaccagaga gcaacuuagu gaggucaaug gugacagcgg     3480

ggucaaccga ucauauggac cacuucucuc uuggagugcu ugugauucua cucauggugc     3540

aggagggguu gaagaagaga augaccacaa agaucaucau gagcacauca auggcagugc     3600

ugguagucau gaucuuggga ggauuuucaa ugagugaccu ggccaagcuu gugauccuga     3660

ugggugcuac uuucgcagaa augaacacug gaggagaugu agcucacuug gcauugguag     3720

cggcauuuaa agucagacca gccuugcugg ucuccuucau uuucagagcc aauuggacac     3780

cccgugagag caugcugcua gcccuggcuu cgugucuucu gcaaacugcg aucucugcuc     3840

uugaagguga cuugaugguc cucauuaaug gauuugcuuu ggccugguug gcaauucgag     3900

caauggccgu gccacgcacu gacaacaucg cucuaccaau cuuggcugcu cuaacaccac     3960

uagcucgagg cacacugcuc guggcaugga gagcgggccu ggcuacuugu ggagggauca     4020

ugcuccucuc ccugaaaggg aaagguagug ugaagaagaa ccugccauuu gucauggccc     4080

ugggauugac agcugugagg guaguagacc cuauuaaugu gguaggacua cuguuacuca     4140

caaggagugg gaagcggagc uggcccccua gugaaguucu cacagccguu ggccugauau     4200

gugcacuggc cggaggguuu gccaaggcag acauugagau ggcuggaccc auggcugcag     4260

uaggcuugcu aauugucagc uauguggucu cgggaaagag uguggacaug uacauugaaa     4320

gagcagguga caucacaugg gaaaaggacg cggaagucac uggaaacagu ccucggcuug     4380

acguggcacu ggaugagagu ggugacuucu ccuugguaga ggaagauggu ccacccauga     4440

gagagaucau acucaaggug guccugaugg ccaucugugg caugaaccca auagcuauac     4500

cuuuugcugc aggagcgugg uauguguaug ugaagacugg gaaaaggagu ggcgcccucu     4560

gggacgugcc ugcucccaaa gaagugaaga aaggagagac cacagaugga guguacagag     4620

ugaugacucg cagacugcua gguucaacac agguuggagu gggagucaug caagagggag     4680

ucuuccacac cauguggcac guuacaaaag gagccgcacu gaggagcggu gagggaagac     4740

uugauccaua cuggggggau gucaagcagg acuugguguc auacuguggg ccuuggaagu     4800

uggaugcagc uugggaugga cucagcgagg uacagcuuuu ggccguaccu cccggagaga     4860

gggccagaaa cauucagacc cugccuggaa uauucaagac aaaggacggg gacaucggag     4920

caguugcucu ggacuacccu gcagggaccu caggaucucc gauccuagac aaauguggaa     4980

gagugauagg acucuauggc aaugggguug ugaucaagaa uggaagcuau guuagugcua     5040

uaacccaggg aaagagggag gaggagacuc cgguugaaug uuucgaaccc ucgaugcuga     5100

agaagaagca gcuaacuguc uuggaucugc auccaggagc cggaaaaacc aggagaguuc     5160

uuccugaaau aguccgugaa gccauaaaaa agagacuccg gacagugauc uuggcaccaa     5220

cuaggguugu cgcugcugag auggaggagg ccuugagagg acuuccggug cguuacauga     5280

caacagcagu caacgucacc cauucuggga cagaaaucgu ugauuugaug ugccaugcca     5340

cuuucacuuc acgcuuacua caacccauca gagucccuaa uuacaaucuc aacaucaugg     5400

augaagccca cuucacagac cccucaagua uagcugcaag aggauacaua ucaacaaggg     5460

uugaaauggg cgaggcggcu gccauuuuua ugacugccac accaccagga acccgugaug     5520

cguuuccuga cucuaacuca ccaaucaugg acacagaagu ggaaguccca gagagagccu     5580

ggagcucagg cuuugauugg gugacagacc auucugggaa aacaguuugg uucguuccaa     5640

gcgugagaaa cggaaaugaa aucgcagccu gucugacaaa ggcuggaaag cgggucauac     5700

agcucagcag gaagacuuuu gagacagaau uucagaaaac aaaaaaucaa gagugggacu     5760

uugucauaac aacugacauc ucagagaugg gcgccaacuu caaggcugac cgggucauag     5820

acucuaggag augccuaaaa ccagucauac uugaugguga gagagucauc uuggcugggc     5880

ccaugccugu cacgcaugcu agugcugcuc agaggagagg acguauaggc aggaacccua     5940

acaaaccugg agaugaguac auguauggag gugggugugc agagacugau gaaggccaug     6000

cacacuggcu ugaagcaaga augcuucuug acaacaucua ccuccaggau ggccucauag     6060

ccucgcucua ucggccugag gccgauaagg uagccgccau ugagggagag uuuaagcuga     6120

ggacagagca aaggaagacc uucguggaac ucaugaagag aggagaccuu cccgucuggc     6180

uagccuauca gguugcaucu gccggaauaa cuuacacaga cagaagaugg ugcuuugaug     6240

gcacaaccaa caacaccaua auggaagaca guguaccagc agagguuugg acaaaguaug     6300

gagagaagag agugcucaaa ccgagaugga uggaugcuag ggucuguuca gaccaugcgg     6360

cccugaaguc guucaaagaa uucgccgcug gaaaaagagg agcggcuuug ggaguaaugg     6420

aggcccuggg aacacugcca ggacacauga cagagagguu ucaggaagcc auugacaacc     6480

ucgccgugcu caugcgagca gagacuggaa gcaggccuua uaaggcagcg gcagcccaac     6540

ugccggagac ccuagagacc auuaugcucu uagguuugcu gggaacaguu ucacugggga     6600

ucuucuucgu cuugaugcgg aauaagggca ucgggaagau gggcuuugga augguaaccc     6660

uuggggccag ugcauggcuc auguggcuuu cggaaauuga accagccaga auugcaugug     6720

uccucauugu uguguuuuua uuacuggugg ugcucauacc cgagccagag aagcaaagau     6780

cuccccaaga uaaccagaug gcaauuauca ucaugguggc agugggccuu cuagguuuga     6840

uaacugcaaa cgaacuugga uggcuggaaa gaacaaaaaa ugacauagcu caucuaaugg     6900

gaaggagaga agaaggagca accaugggau ucucaaugga cauugaucug cggccagccu     6960

ccgccugggc uaucuaugcc gcauugacaa cucucaucac cccagcuguc caacaugcgg     7020

uaaccacuuc auacaacaac uacuccuuaa uggcgauggc cacacaagcu ggagugcugu     7080

uuggcauggg caaagggaug ccauuuaugc auggggaccu uggagucccg cugcuaauga     7140

uggguugcua uucacaauua acaccccuga cucugauagu agcuaucauu cugcuugugg     7200

cgcacuacau guacuugauc ccaggccuac aagcggcagc agcgcgugcu gcccagaaaa     7260

ggacagcagc uggcaucaug aagaaucccg uuguggaugg aauaguggua acugacauug     7320

acacaaugac aauagacccc cagguggaga agaagauggg acaaguguua cucauagcag     7380

uagccaucuc cagugcugug cugcugcgga ccgccugggg auggggggag gcuggagcuc     7440

ugaucacagc agcgaccucc accuuguggg aaggcucucc aaacaaauac uggaacuccu     7500

cuacagccac cucacugugc aacaucuuca gaggaagcua ucuggcagga gcuucccuua     7560

ucuauacagu gacgagaaac gcuggccugg uuaagagacg uggagguggg acgggagaga     7620

cucugggaga gaaguggaaa gcucgucuga aucagauguc ggcccuggag uucuacucuu     7680

auaaaaaguc agguaucacu gaagugugua gagaggaggc ucgccgugcc cucaaggaug     7740

gaguggccac aggaggacau gccguauccc ggggaagugc aaagaucaga ugguuggagg     7800

agagaggaua ucugcagccc uaugggaagg uuguugaccu cggauguggc agagggggcu     7860

ggagcuauua ugccgccacc auccgcaaag ugcaggaggu gagaggauac acaaagggag     7920

gucccgguca ugaagaaccc augcuggugc aaagcuaugg guggaacaua guucgucuca     7980

agaguggagu ggacgucuuc cacauggcgg cugagccgug ugacacucug cugugugaca     8040

uaggugaguc aucaucuagu ccugaagugg aagagacacg aacacucaga gugcucucua     8100

ugguggggga cuggcuugaa aaaagaccag gggccuucug uauaaaggug cugugcccau     8160

acaccagcac uaugauggaa accauggagc gacugcaacg uaggcauggg ggaggauuag     8220

ucagagugcc auugugucgc aacuccacac augagaugua cugggucucu ggggcaaaga     8280

gcaacaucau aaaaagugug uccaccacaa gucagcuccu ccugggacgc auggauggcc     8340

ccaggaggcc agugaaauau gaggaggaug ugaaccucgg cucggguaca cgagcugugg     8400

caagcugugc ugaggcuccu aacaugaaaa ucaucggcag gcgcauugag agaauccgca     8460

augaacaugc agaaacaugg uuucuugaug aaaaccaccc auacaggaca ugggccuacc     8520

augggagcua cgaagccccc acgcaaggau cagcgucuuc ccucgugaac gggguuguua     8580

gacuccuguc aaagccuugg gacgugguga cuggaguuac aggaauagcc augacugaca     8640

ccacaccaua cggccaacaa agagucuuca aagaaaaagu ggacaccagg gugccagauc     8700

cccaagaagg cacucgccag guaaugaaca uagucucuuc cuggcugugg aaggagcugg     8760

ggaaacgcaa gcggccacgc gucugcacca aagaagaguu uaucaacaag gugcgcagca     8820

augcagcacu gggagcaaua uuugaagagg aaaaagaaug gaagacggcu guggaagcug     8880

ugaaugaucc aagguuuugg gcccuagugg auagggagag agaacaccac cugagaggag     8940

agugucacag cuguguguac aacaugaugg gaaaaagaga aaagaagcaa ggagaguucg     9000

ggaaagcaaa agguagccgc gccaucuggu acaugugguu gggagccaga uucuuggagu     9060

uugaagcccu uggauucuug aacgaggacc auuggauggg aagagaaaac ucaggaggug     9120

gagucgaagg guuaggauug caaagacuug gauacauucu agaagaaaug aaucgggcac     9180

caggaggaaa gauguacgca gaugacacug cuggcuggga cacccgcauu aguaaguuug     9240

aucuggagaa ugaagcucug auuaccaacc aaauggagga agggcacaga acucuggcgu     9300

uggccgugau uaaauacaca uaccaaaaca aaguggugaa gguucucaga ccagcugaag     9360

gaggaaaaac aguuauggac aucauuucaa gacaagacca gagagggagu ggacaaguug     9420

ucacuuaugc ucucaacaca uucaccaacu ugguggugca gcuuauccgg aacauggaag     9480

cugaggaagu guuagagaug caagacuuau gguuguugag gaagccagag aaagugacca     9540

gaugguugca gagcaaugga ugggauagac ucaaacgaau ggcggucagu ggagaugacu     9600

gcguugugaa gccaaucgau gauagguuug cacaugcccu cagguucuug aaugacaugg     9660

gaaaaguuag gaaagacaca caggagugga aacccucgac uggauggagc aauugggaag     9720

aagucccguu cugcucccac cacuucaaca agcuguaccu caaggauggg agauccauug     9780

uggucccuug ccgccaccaa gaugaacuga uuggccgagc ucgcgucuca ccaggggcag     9840

gauggagcau ccgggagacu gccugucuug caaaaucaua ugcgcagaug uggcagcucc     9900

uuuauuucca cagaagagac cuucgacuga uggcuaaugc cauuugcucg gcugugccag     9960

uugacugggu accaacuggg agaaccaccu ggucaaucca uggaaaggga gaauggauga    10020

ccacugagga caugcucaug guguggaaua gaguguggau ugaggagaac gaccauaugg    10080

aggacaagac uccuguaaca aaauggacag acauucccua ucuaggaaaa agggaggacu    10140

uauggugugg aucccuuaua gggcacagac cccgcaccac uugggcugaa aacaucaaag    10200

acacagucaa cauggugcgc aggaucauag gugaugaaga aaaguacaug gacuaucuau    10260

ccacccaagu ccgcuacuug ggugaggaag gguccacacc cggaguguug uaaggacuag    10320

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc    10380

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa    10440

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga    10500

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc    10620

ucuuuucaga gccaccagaa uu                                             10642


<210>  297
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 wild type

<400>  297
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg     1140

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga agcuuaggac     1200

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca     1260

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uucaaggaug     1380

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg     1440

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc     1500

auuugaaaug ccgccuaaaa auggacaagc uuagauugaa gggcguguca uauuccuugu     1560

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1620

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1680

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1740

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1800

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1860

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1920

ccugggacuu cggaucaguc gggggugugu ucaacucacu ggguaagggc auucaccaga     1980

uuuuuggagc agccuucaaa ucacuguuug gaggaauguc cugguucuca cagauccuca     2040

uaggcacgcu gcuagugugg uuagguuuga acacaaagaa uggaucuauc ucccucacau     2100

gcuuggcccu ggggggagug augaucuucc ucuccacggc uguuucugcu gacguggggu     2160

gcucagugga cuucucaaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  298
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 wild type

<400>  298
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg     1140

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga aguuuaggac     1200

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca     1260

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uuuaaggaug     1380

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg     1440

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc     1500

auuugaaaug ccgccuaaaa auggacaagc ucagauugaa gggcguguca uauuccuugu     1560

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1620

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1680

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1740

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1800

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1860

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1920

ccugggacuu cggaucaguc gggggugugu ucaacucacu ggguaagggc auucaccaga     1980

uuuuuggagc agccuucaaa ucacuguuug gaggaauguc cugguucuca cagauccuca     2040

uaggcacgcu gcuagugugg uuagguuuga acacaaagaa uggaucuauc ucccucacau     2100

gcuuggcccu ggggggagug augaucuucc ucuccacggc uguuucugcu gacguggggu     2160

gcucagugga cuucucaaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  299
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 wild type

<400>  299
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      240

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      420

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      480

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc      540

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg      600

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga agcuuaggac      660

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca      720

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug      780

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uucaaggaug      840

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg      900

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc      960

auuugaaaug ccgccuaaaa auggacaagc uuagauugaa gggcguguca uauuccuugu     1020

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1080

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1140

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1200

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1260

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1320

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1380

ccugggacuu cggaucauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  300
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 wild type

<400>  300
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      240

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      420

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      480

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc      540

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg      600

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga aguuuaggac      660

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca      720

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug      780

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uuuaaggaug      840

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg      900

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc      960

auuugaaaug ccgccuaaaa auggacaagc ucagauugaa gggcguguca uauuccuugu     1020

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1080

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1140

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1200

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1260

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1320

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1380

ccugggacuu cggaucauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  301
<211>  10654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein mRNA Design 2 GC

<400>  301
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

accccaagaa gaagagcggc ggguuccgca ucgugaacau gcugaagcgg ggcgucgccc      120

gcgugucccc guucgggggc cucaagcggc ugcccgccgg gcugcuccug ggccacggcc      180

ccauccgcau ggugcuggcg auccucgccu uccugcgguu caccgccauc aagcccagcc      240

uggggcucau caaccgcugg ggcuccgucg ggaagaagga ggccauggag aucaucaaga      300

aguucaagaa ggaccuggcc gcgaugcugc ggaucaucaa cgcccgcaag gagaagaagc      360

ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc gccauggcgg      420

ccgaggucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac gacgccgggg      480

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      540

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      600

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      660

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      720

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      780

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      840

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      900

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      960

aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc ugcgugaccg     1020

ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc gugagcaaca     1080

uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc     1140

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgugugca     1200

agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc     1260

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc auccagccgg     1320

agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc gggaugaucg     1380

ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1440

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1500

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1560

ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1620

ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc     1680

agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1740

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1800

ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc gccgccuuca     1860

ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag gugcaguacg     1920

ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug cagacccuga     1980

cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc gagaacagca     2040

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcguggggg     2100

agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     2160

ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg gacuucggca     2220

gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     2280

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acgcugcuga     2340

uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc gcccugggcg     2400

gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugcucc gucgacuuca     2460

gcaagaagga gacccgcugc gggacgggcg uguucgugua caacgacguc gaggccuggc     2520

gggaccgcua caaguaccac cccgacuccc cgcgccggcu ggccgccgcg gugaagcagg     2580

ccugggagga cgggaucugc ggcaucagcu ccgugagccg cauggagaac aucauguggc     2640

ggagcgucga gggggagcug aacgccaucc ucgaggagaa cggcgugcag cugaccgugg     2700

ucgugggcuc cgugaagaac ccgauguggc gcgggcccca gcggcugccc gucccgguga     2760

acgagcuccc ccacggcugg aaggccuggg ggaagagcca cuucgugcgc gcggccaaga     2820

ccaacaacuc cuucgucgug gacggcgaca cccugaagga gugcccccug aagcaccggg     2880

ccuggaacag cuuccucgug gaggaccacg gguucggcgu cuuccacacg uccguguggc     2940

ugaaggugcg cgaggacuac agccuggagu gcgaccccgc cgucaucggc accgccguga     3000

aggggaagga ggcggugcac uccgaccucg gcuacuggau cgagagcgag aagaacgaca     3060

ccuggcggcu gaagcgcgcc caccugaucg agaugaagac cugcgagugg ccgaaguccc     3120

acacgcucug gaccgacggg aucgaggaga gcgaccugau cauccccaag ucccuggccg     3180

gcccccucag ccaccacaac acccgggagg gguaccgcac ccagaugaag ggccccuggc     3240

acuccgagga gcuggagauc cgguucgagg agugcccggg cacgaagguc cacguggagg     3300

agaccugcgg gacccgcggc cccagccugc gguccaccac ggccagcggg cgcgucaucg     3360

aggaguggug cugccgggag ugcaccaugc ccccgcucuc cuuccgcgcc aaggacggcu     3420

gcugguacgg cauggagauc cggccgcgca aggagcccga gagcaaccug gugcggucca     3480

uggugaccgc ggggagcacc gaccacaugg accacuucag ccugggcguc cucgugaucc     3540

ugcugauggu gcaggagggg cucaagaagc gcaugacgac caagaucauc aucuccacca     3600

gcauggccgu ccugguggcc augauccugg gcggguucuc caugagcgac cucgccaagc     3660

uggccauccu gaugggcgcg accuucgccg agaugaacac cggcggggac guggcccacc     3720

ucgcccugau cgccgcguuc aagguccgcc ccgcccugcu cguguccuuc aucuuccggg     3780

ccaacuggac gccccgcgag agcaugcugc uggcccucgc guccugccug cugcagaccg     3840

ccaucagcgc ccucgagggc gaccugaugg ugcugaucaa cggguucgcc cucgccuggc     3900

uggcgauccg ggccaugguc gugccgcgca ccgacaacau cacccuggcc auccucgccg     3960

cccugacgcc ccuggcgcgg ggcacccucc ugguggccug gcgcgccggg cuggccacgu     4020

gcggcggcuu caugcuccug ucccugaagg ggaagggcag cgucaagaag aaccuccccu     4080

ucgugauggc ccuggggcug accgcggugc ggcucgucga ccccaucaac guggugggcc     4140

ugcugcuccu gacgcgcucc gggaagcgga gcuggccgcc cuccgagguc cugaccgccg     4200

ugggccucau cugcgcccug gccggcgggu ucgcgaaggc cgacaucgag auggccggcc     4260

ccauggccgc cguggggcug cucaucguca gcuacguggu guccggcaag agcgucgaca     4320

uguacaucga gcgcgcgggg gacaucaccu gggagaagga cgccgaggug accggcaacu     4380

ccccgcggcu ggacguggcc cuggacgaga gcggcgacuu cucccucguc gaggacgacg     4440

ggccgcccau gcgcgagauc auccugaagg uggugcugau gacgaucugc ggcaugaacc     4500

ccaucgccau cccguucgcc gcgggggccu gguacgucua cgugaagacc ggcaagcgga     4560

gcggcgcccu gugggacgug cccgccccca aggaggucaa gaagggggag accaccgacg     4620

gcguguaccg cgugaugacg cggcgccucc uggggagcac ccaggucggc guggggguga     4680

ugcaggaggg cgucuuccac accauguggc acgugaccaa gggcuccgcc cugcggagcg     4740

gggagggccg ccucgacccc uacuggggcg acgugaagca ggaccugguc uccuacugcg     4800

ggccguggaa gcuggacgcg gccugggacg ggcacagcga ggugcagcuc cuggccgugc     4860

ccccgggcga gcgggcccgc aacauccaga cgcugcccgg caucuucaag accaaggacg     4920

gggacaucgg cgcggucgcc cucgacuacc cggccgggac cuccggcagc cccauccugg     4980

acaagugcgg gcgcgugauc ggccuguacg gcaacggggu ggucaucaag aacggcuccu     5040

acgugagcgc caucacccag gggcggcgcg aggaggagac gcccgucgag ugcuucgagc     5100

ccuccaugcu caagaagaag cagcugaccg ugcuggaccu ccacccgggc gccgggaaga     5160

cccggcgcgu gcugcccgag aucguccggg aggcgaucaa gacccgccug cggacgguga     5220

uccucgcccc cacccgcgug gucgccgccg agauggagga ggcccugcgg ggccugcccg     5280

ugcgcuacau gaccaccgcg gugaacguca cgcacagcgg caccgagauc guggaccuca     5340

ugugccacgc caccuucacc ucccggcugc ugcagccgau ccgcgugccc aacuacaacc     5400

ucuacaucau ggacgaggcc cacuucacgg accccagcuc caucgccgcc cggggguaca     5460

ucagcacccg cgucgagaug ggcgaggcgg ccgccaucuu caugaccgcc accccgccgg     5520

ggacccggga cgccuucccc gacuccaaca gccccaucau ggacacggag guggaggugc     5580

ccgagcgcgc gugguccagc ggcuucgacu gggucaccga cuacuccggc aagaccgugu     5640

gguucgugcc gagcguccgg aacgggaacg agaucgccgc cugccugacc aaggccggca     5700

agcgcgugau ccagcugagc cggaagacgu ucgagaccga guuccagaag accaagcacc     5760

aggaguggga cuucgugguc accacggaca ucuccgagau gggggcgaac uucaaggccg     5820

accgcgugau cgacagccgg cgcugccuca agcccgugau ccuggacggc gagcgcguca     5880

uccuggccgg gcccaugccc gugacccacg ccuccgccgc gcagcggcgc ggccggaucg     5940

gccgcaaccc gaacaagccc ggggacgagu accucuacgg cgggggcugc gccgagaccg     6000

acgaggacca cgcccacugg cuggaggccc ggaugcugcu cgacaacauc uaccugcagg     6060

acgggcugau cgccagccuc uaccgccccg aggcggacaa gguggccgcc aucgagggcg     6120

aguucaagcu gcggaccgag cagcgcaaga cguucgucga gcugaugaag cggggcgacc     6180

ucccggugug gcuggccuac cagguggccu ccgcggggau caccuacacc gaccgccggu     6240

ggugcuucga cggcaccacg aacaacacca ucauggagga cagcgucccc gccgaggugu     6300

ggacccgcca cggggagaag cgggugcuga agccccgcug gauggacgcc cgggucugcu     6360

ccgaccacgc cgcgcucaag agcuucaagg aguucgccgc cggcaagcgc ggggccgccu     6420

ucggcgugau ggaggcgcug ggcacccugc ccgggcacau gacggagcgg uuccaggagg     6480

ccaucgacaa ccucgccgug cugaugcgcg ccgagaccgg cucccggccg uacaaggccg     6540

cggccgccca gcugcccgag acccucgaga ccaucaugcu gcuggggcuc cugggcacgg     6600

ucagccuggg caucuucuuc gugcucaugc gcaacaaggg gaucggcaag augggguucg     6660

gcauggugac ccugggggcc uccgccuggc ugauguggcu cagcgagauc gagcccgcgc     6720

gcaucgccug cguccugauc gugguguucc ugcuccuggu cgugcugauc cccgagccgg     6780

agaagcagcg guccccgcag gacaaccaga uggccaucau caucauggug gccgucggcc     6840

uccugggccu gaucaccgcg aacgagcucg gguggcugga gcgcaccaag agcgaccugu     6900

cccaccucau gggccggcgc gaggaggggg ccacgauggg cuucagcaug gacaucgacc     6960

ugcggcccgc cuccgccugg gccaucuacg cggcccugac caccuucauc acccccgccg     7020

ugcagcacgc cgugacgacc agcuacaaca acuacucccu cauggccaug gcgacccagg     7080

ccgggguccu guucggcaug ggcaagggga ugccguucua cgccugggac uucggcgugc     7140

cgcugcucau gaucgggugc uacagccagc ugaccccgcu gacgcucauc guggccauca     7200

uccugcuggu cgcccacuac auguaccuca uccccggccu gcaggcggcc gccgcccgcg     7260

ccgcgcagaa gcggaccgcc gccgggauca ugaagaaccc gguggucgac ggcaucgugg     7320

ugaccgacau cgacaccaug acgaucgacc cccaggucga gaagaagaug ggccaggugc     7380

ugcucauggc cguggcgguc agcuccgcca uccugagccg caccgccugg ggguggggcg     7440

aggccggggc ccugaucacc gcggccaccu ccacccucug ggagggcagc cccaacaagu     7500

acuggaacuc cagcacggcc accucccugu gcaacaucuu ccgggggagc uaccuggccg     7560

gcgccucccu caucuacacc gugacccgca acgcgggccu ggugaagcgg cgcgggggcg     7620

ggacgggcga gacccugggc gagaagugga aggcccggcu caaccagaug agcgcccugg     7680

aguucuacuc cuacaagaag agcgggauca ccgaggucug ccgcgaggag gcccggcgcg     7740

cccugaagga cggcguggcg accgggggcc acgccguguc ccgggggagc gccaagcucc     7800

gcuggcuggu cgagcggggc uaccugcagc ccuacggcaa ggugaucgac cuggggugcg     7860

gccgcggggg cugguccuac uacgccgcga cgauccggaa ggugcaggag gucaaggggu     7920

acaccaaggg cggcccgggg cacgaggagc ccgugcucgu gcagagcuac ggcuggaaca     7980

ucguccgccu gaaguccggg guggacgugu uccacauggc cgccgagccc ugcgacaccc     8040

ugcucugcga caucggcgag agcuccagca gccccgaggu cgaggaggcc cgcacccugc     8100

gggugcuguc cauggugggg gacuggcucg agaagcgccc gggcgccuuc ugcaucaagg     8160

uccugugccc cuacacgagc accaugaugg agacccugga gcggcuccag cgccgguacg     8220

gcgggggccu ggugcgcgug ccccuguccc ggaacagcac ccacgagaug uacugggucu     8280

ccggggcgaa gagcaacacg aucaaguccg ugagcaccac cucccagcuc cugcugggcc     8340

gcauggacgg cccccggcgc ccggugaagu acgaggagga cgucaaccuc gggagcggca     8400

cccgggccgu gguguccugc gccgaggccc ccaacaugaa gaucaucggg aaccgcaucg     8460

agcggauccg cagcgagcac gccgagacgu gguucuucga cgagaaccac cccuaccgga     8520

ccugggcgua ccacggcucc uacgaggccc cgacccaggg gagcgccucc agccugauca     8580

acggcgucgu gcgccugcuc uccaagcccu gggacguggu caccggcgug acggggaucg     8640

ccaugaccga caccaccccc uacggccagc agcggguguu caaggagaag gucgacacgc     8700

gcgugcccga cccgcaggag gggacccgcc aggugaugag cauggucagc uccuggcugu     8760

ggaaggagcu gggcaagcac aagcgccccc gcgugugcac caaggaggag uucaucaaca     8820

aggugcggag caacgccgcg cucggggcca ucuucgagga ggagaaggag uggaagaccg     8880

ccgucgaggc cgugaacgac ccccgcuucu gggcccuggu ggacaaggag cgggagcacc     8940

accuccgcgg cgagugccag uccugcgucu acaacaugau gggcaagcgg gagaagaagc     9000

agggggaguu cggcaaggcg aaggggagcc gcgccaucug guacaugugg cucggcgccc     9060

gguuccugga guucgaggcc cugggguucc ucaacgagga ccacuggaug ggccgcgaga     9120

acuccggcgg gggcguggag gggcugggcc ugcagcggcu cggguacgug cuggaggaga     9180

ugagccgcau ccccggcggc cggauguacg cggacgacac ggccgggugg gacacccgca     9240

ucucccgguu cgaccuggag aacgaggccc ucaucaccaa ccagauggag aagggccacc     9300

gcgcccuggc ccuggcgauc aucaaguaca ccuaccagaa caaggucgug aagguccucc     9360

ggccggccga gaaggggaag acggugaugg acaucaucag ccgccaggac cagcggggcu     9420

ccggccaggu ggucaccuac gcccugaaca ccuucaccaa ccugguggug cagcucaucc     9480

gcaacaugga ggccgaggag guccuggaga ugcaggaccu guggcuccug cggcgcagcg     9540

agaaggugac caacuggcug caguccaacg ggugggaccg gcucaagcgc auggccguga     9600

gcggcgacga cugcgucgug aagcccaucg acgaccgcuu cgcgcacgcc cugcgguucc     9660

ugaacgacau ggggaaggug cgcaaggaca cgcaggagug gaagcccucc accggcuggg     9720

acaacuggga ggaggucccc uucugcagcc accacuucaa caagcuccac cugaaggacg     9780

ggcgguccau cguggugccg ugccgccacc aggacgagcu gaucggccgg gcccgcguca     9840

gccccggcgc cggguggucc auccgggaga ccgccugccu cgcgaagagc uacgcccaga     9900

uguggcagcu gcuguacuuc caccgccggg accuccgccu gauggccaac gccaucugcu     9960

ccagcgugcc cguggacugg guccccaccg gccggacgac cuggagcauc cacgggaagg    10020

gcgaguggau gaccaccgag gacaugcugg ugguguggaa ccgcgucugg aucgaggaga    10080

acgaccacau ggaggacaag acgccgguga ccaaguggac cgacaucccc uaccucggga    10140

agcgggagga ccuguggugc ggcucccuga ucggccaccg cccccggacc acgugggcgg    10200

agaacaucaa gaacaccgug aacauggucc gccggaucau cggggacgag gagaaguaca    10260

uggacuaccu cagcacccag gugcgcuacc ugggcgagga gggguccacc cccggcgugc    10320

ugugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag    10380

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac    10440

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua    10500

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc    10620

ccccccaaag gcucuuuuca gagccaccag aauu                                10654


<210>  302
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5050/R5051/R4798 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) 
       mRNA Design 2 GC

<400>  302
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcucc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      720

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      780

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1680

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1740

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc gcggugagcg     2160

ccgacguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  303
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 GC

<400>  303
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacaucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagcccacc guggacaucg      780

agcuggugac caccacgguc agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu ggagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcaccc cgcacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccguggucgu gcucgggucc caggagggcg     1440

ccgugcacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguca     1560

gcuacucccu gugcacggcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu ggacaugcag acccugaccc ccgucgggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg ccguucggcg     1800

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccugaacucc cucgggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  304
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5054/R5055 ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA 
       Design 2 GC

<400>  304
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      120

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      180

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      240

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      360

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      420

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgugc      540

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      660

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      720

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc      780

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg      900

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc      960

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1020

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1080

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1140

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1200

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1320

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1380

ugggggacac cgcgugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  305
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 GC

<400>  305
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacaucg      180

ugcuggagca cggcgggugc gugaccguca uggcgcagga caagccgacg guggacaucg      240

agcucgugac caccaccguc agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      420

gcugcgggcu cuucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgugc      540

acggcuccca gcacagcggg augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcug ggcggguucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcg aagcgccaga ccgugguggu ccuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguga     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgucac gguggagguc caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc cggucgggcg ccucaucacg gccaacccgg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  306
<211>  10654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein mRNA Design 2 GC

<400>  306
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

accccaagaa gaagagcggc ggguuccgca ucgugaacau gcugaagcgg ggcgucgccc      120

gcgugucccc guucgggggc cucaagcggc ugcccgccgg gcugcuccug ggccacggcc      180

ccauccgcau ggugcuggcg auccucgccu uccugcgguu caccgccauc aagcccagcc      240

uggggcucau caaccgcugg ggcuccgucg ggaagaagga ggccauggag aucaucaaga      300

aguucaagaa ggaccuggcc gcgaugcugc ggaucaucaa cgcccgcaag gagaagaagc      360

ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc gccauggcgg      420

ccgaggucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac gacgccgggg      480

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      540

uggggcacac gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      600

agcccgacga cguggacugc uggugcaaca ccacgagcac cugggucgug uacggcacgu      660

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      720

ccacgcgcaa gcugcagacc cggagccaga ccuggcucga gucccgcgag uacaccaagc      780

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      840

ccaucgccug gcuccugggg agcuccacga gccagaaggu gaucuaccug gugaugaucc      900

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      960

aggggauguc cggcggcacc uggguggacg ucgugcugga gcacgggggc ugcgugaccg     1020

ucauggccca ggacaagccc accguggaca ucgagcucgu gacgaccacc gucagcaaca     1080

uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc     1140

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacgcag uacgucugca     1200

agcgcacccu gguggaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc     1260

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc auccagccgg     1320

agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc gggaugaucg     1380

ugaacgacac gggccacgag accgacgaga accgggccaa gguggagauc acccccaaca     1440

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1500

ggacgggccu ggacuucagc gaccuguacu accucaccau gaacaacaag cacuggcugg     1560

uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1620

ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac gccaagcgcc     1680

agacgguggu cgugcugggc ucccaggagg gggccgugca caccgcccug gccggcgcgc     1740

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1800

ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc gccgccuuca     1860

ccuucaccaa gauccccgcg gagacgcucc acggcaccgu gaccguggag guccaguacg     1920

ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug cagacgcuga     1980

cccccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacg gagaacagca     2040

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcgucgggg     2100

agaagaagau cacccaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     2160

ccaccgugcg cggcgccaag cggauggccg ugcuggggga cacggcgugg gacuucggca     2220

gcgucggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     2280

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acccugcuga     2340

uguggcucgg gcugaacgcc aagaacggcu ccaucagccu gaugugccuc gcgcugggcg     2400

gcgugcugau cuuccucucc accgccguga gcgccgacgu cgggugcucc guggacuuca     2460

gcaagaagga gacccgcugc gggacgggcg uguucgucua caacgacgug gaggccuggc     2520

gggaccgcua caaguaccac cccgacuccc cgcgccggcu ggccgcggcc gugaagcagg     2580

ccugggagga cgggaucugc ggcaucagcu ccgucagccg cauggagaac aucauguggc     2640

ggagcgugga gggggagcug aacgccaucc ucgaggagaa cggcgugcag cugaccgucg     2700

uggugggcuc cgucaagaac ccgauguggc gcgggcccca gcggcugccc gugccgguga     2760

acgagcuccc ccacggcugg aaggcguggg ggaagagcua cuucguccgc gccgccaaga     2820

ccaacaacuc cuucguggug gacggcgaca cccugaagga gugcccccug aagcaccggg     2880

ccuggaacag cuuccucguc gaggaccacg gguucggcgu guuccacacg uccguguggc     2940

ugaagguccg cgaggacuac agccuggagu gcgaccccgc cgugaucggc accgcgguga     3000

aggggaagga ggccguccac uccgaccucg gcuacuggau cgagagcgag aagaacgaca     3060

ccuggcggcu gaagcgcgcc caccugaucg agaugaagac cugcgagugg ccgaaguccc     3120

acacgcucug gaccgacggg aucgaggaga gcgaccugau cauccccaag ucccuggccg     3180

gcccccucag ccaccacaac acccgggagg gguaccgcac ccagaugaag ggccccuggc     3240

acuccgagga gcuggagauc cgguucgagg agugcccggg cacgaaggug cacgucgagg     3300

agaccugcgg gacccgcggc cccagccugc gguccaccac ggccagcggg cgcgugaucg     3360

aggaguggug cugccgggag ugcaccaugc cgccccucuc cuuccgcgcg aaggacggcu     3420

gcugguacgg cauggagauc cggccgcgca aggagcccga gagcaaccug gugcggucca     3480

uggucaccgc cgggagcacc gaccacaugg accacuucag ccugggcgug cucgugaucc     3540

ugcugauggu ccaggagggg cucaagaagc gcaugacgac caagaucauc aucuccacca     3600

gcauggccgu gcugguggcc augauccugg gcggguucuc caugagcgac cucgccaagc     3660

uggcgauccu gaugggcgcc accuucgccg agaugaacac cggcggggac gucgcccacc     3720

ucgcccugau cgcggccuuc aaggugcgcc ccgcccugcu cguguccuuc aucuuccggg     3780

ccaacuggac gccccgcgag agcaugcugc uggcgcucgc cuccugccug cugcagaccg     3840

ccaucagcgc ccucgagggc gaccugaugg uccugaucaa cggguucgcc cucgcguggc     3900

uggccauccg ggccauggug gugccgcgca ccgacaacau cacccuggcc auccucgccg     3960

cgcugacgcc ccuggcccgg ggcacccucc uggucgccug gcgcgccggg cuggccacgu     4020

gcggcggcuu caugcuccug ucccugaagg ggaagggcag cgugaagaag aaccuccccu     4080

ucgugauggc gcuggggcug accgccgucc ggcucgugga ccccaucaac guggucggcc     4140

ugcugcuccu gacgcgcucc gggaagcgga gcuggccgcc cuccgaggug cugaccgccg     4200

ugggccucau cugcgcccug gcgggcgggu ucgccaaggc cgacaucgag auggccggcc     4260

ccauggccgc ggucgggcug cucaucguga gcuacguggu cuccggcaag agcguggaca     4320

uguacaucga gcgcgccggg gacaucaccu gggagaagga cgccgaggug accggcaacu     4380

ccccgcggcu ggacgucgcc cuggacgaga gcggcgacuu cucccucgug gaggacgacg     4440

ggccgcccau gcgcgagauc auccugaagg ugguccugau gacgaucugc ggcaugaacc     4500

ccaucgccau cccguucgcg gccggggccu gguacgugua cgugaagacc ggcaagcgga     4560

gcggcgcccu gugggacguc cccgccccca aggaggugaa gaagggggag accaccgacg     4620

gcguguaccg cgucaugacg cggcgccucc uggggagcac ccaggugggc guggggguca     4680

ugcaggaggg cguguuccac accauguggc acgugaccaa gggcuccgcg cugcggagcg     4740

gggagggccg ccucgacccc uacuggggcg acgucaagca ggaccuggug uccuacugcg     4800

ggccguggaa gcuggacgcc gccugggacg ggcacagcga ggugcagcuc cuggccgucc     4860

cgcccggcga gcgggcgcgc aacauccaga cgcugcccgg caucuucaag accaaggacg     4920

gggacaucgg cgccguggcc cucgacuacc cggccgggac cuccggcagc cccauccugg     4980

acaagugcgg gcgcgugauc ggccuguacg gcaacggggu cgugaucaag aacggcuccu     5040

acgucagcgc caucacccag gggcggcgcg aggaggagac gcccguggag ugcuucgagc     5100

ccuccaugcu caagaagaag cagcugaccg ugcuggaccu ccacccgggc gcggggaaga     5160

cccggcgcgu ccugcccgag aucgugcggg aggccaucaa gacccgccug cggacgguga     5220

uccucgcccc cacccgcguc guggccgccg agauggagga ggcgcugcgg ggccugcccg     5280

ugcgcuacau gaccaccgcc gucaacguga cgcacagcgg caccgagauc guggaccuca     5340

ugugccacgc caccuucacc ucccggcugc ugcagccgau ccgcgucccc aacuacaacc     5400

ucuacaucau ggacgaggcc cacuucacgg accccagcuc caucgccgcg cggggguaca     5460

ucagcacccg cguggagaug ggcgaggccg ccgccaucuu caugaccgcc accccgccgg     5520

ggacccggga cgcguucccc gacuccaaca gccccaucau ggacacggag guggaggucc     5580

ccgagcgcgc cugguccagc ggcuucgacu gggugaccga ccacuccggc aagaccgugu     5640

gguucguccc gagcgugcgg aacgggaacg agaucgccgc cugccugacc aaggcgggca     5700

agcgcgugau ccagcugagc cggaagacgu ucgagaccga guuccagaag accaagcacc     5760

aggaguggga cuucgucgug accacggaca ucuccgagau gggggccaac uucaaggccg     5820

accgcgugau cgacagccgg cgcugccuca agcccgucau ccuggacggc gagcgcguga     5880

uccuggccgg gcccaugccc gugacccacg ccuccgcggc ccagcggcgc ggccggaucg     5940

gccgcaaccc gaacaagccc ggggacgagu accucuacgg cgggggcugc gccgagaccg     6000

acgaggacca cgcccacugg cuggaggccc ggaugcugcu cgacaacauc uaccugcagg     6060

acgggcugau cgcgagccuc uaccgccccg aggccgacaa ggucgccgcc aucgagggcg     6120

aguucaagcu gcggaccgag cagcgcaaga cguucgugga gcugaugaag cggggcgacc     6180

ucccggugug gcuggccuac caggucgcgu ccgccgggau caccuacacc gaccgccggu     6240

ggugcuucga cggcaccacg aacaacacca ucauggagga cagcgugccc gccgaggugu     6300

ggacccgcca cggggagaag cggguccuga agccccgcug gauggacgcc cgggugugcu     6360

ccgaccacgc ggcccucaag agcuucaagg aguucgccgc cggcaagcgc ggggccgcgu     6420

ucggcgugau ggaggcccug ggcacccugc ccgggcacau gacggagcgg uuccaggagg     6480

ccaucgacaa ccucgccguc cugaugcgcg ccgagaccgg cucccggccg uacaaggcgg     6540

ccgccgccca gcugcccgag acccucgaga ccaucaugcu gcuggggcuc cugggcacgg     6600

ugagccuggg caucuucuuc gugcucaugc gcaacaaggg gaucggcaag augggguucg     6660

gcauggucac ccugggggcc uccgcguggc ugauguggcu cagcgagauc gagcccgccc     6720

gcaucgccug cgugcugauc guggucuucc ugcuccuggu ggugcugauc cccgagccgg     6780

agaagcagcg guccccgcag gacaaccaga uggccaucau caucaugguc gcggugggcc     6840

uccugggccu gaucaccgcc aacgagcucg gguggcugga gcgcaccaag agcgaccugu     6900

cccaccucau gggccggcgc gaggaggggg ccacgaucgg cuucagcaug gacaucgacc     6960

ugcggcccgc cuccgccugg gcgaucuacg ccgcccugac caccuucauc acccccgccg     7020

ugcagcacgc cgucacgacc agcuacaaca acuacucccu cauggcgaug gccacccagg     7080

ccggggugcu guucggcaug ggcaagggga ugccguucua cgccugggac uucggcgugc     7140

cccugcucau gaucgggugc uacagccagc ugaccccgcu gacgcucauc gucgccauca     7200

uccugcuggu ggcgcacuac auguaccuca uccccggccu gcaggccgcc gccgcccgcg     7260

cggcccagaa gcggaccgcc gccgggauca ugaagaaccc ggucguggac ggcaucgugg     7320

ucaccgacau cgacaccaug acgaucgacc cccaggugga gaagaagaug ggccaggugc     7380

ugcucaucgc ggucgccgug agcuccgcca uccugagccg caccgccugg ggguggggcg     7440

aggccggggc gcugaucacc gccgccaccu ccacccucug ggagggcagc cccaacaagu     7500

acuggaacuc cagcacggcc accucccugu gcaacaucuu ccgggggagc uaccuggccg     7560

gcgcgucccu caucuacacc gugacccgca acgccggccu ggucaagcgg cgcgggggcg     7620

ggacgggcga gacccugggc gagaagugga aggcccggcu caaccagaug agcgcccugg     7680

aguucuacuc cuacaagaag agcgggauca ccgaggugug ccgcgaggag gcccggcgcg     7740

cgcugaagga cggcguggcc accgggggcc acgccgucuc ccgggggagc gccaagcucc     7800

gcuggcuggu ggagcggggc uaccugcagc ccuacggcaa ggugaucgac cuggggugcg     7860

gccgcggggg cugguccuac uacgcggcca cgauccggaa gguccaggag gugaaggggu     7920

acaccaaggg cggcccgggg cacgaggagc ccgugcucgu ccagagcuac ggcuggaaca     7980

ucgugcgccu gaaguccggg guggacgucu uccacauggc cgccgagccc ugcgacaccc     8040

ugcucugcga caucggcgag agcuccagca gccccgaggu ggaggaggcc cgcacccugc     8100

gggugcuguc cauggucggg gacuggcucg agaagcgccc gggcgcguuc ugcaucaagg     8160

ugcugugccc cuacacgagc accaugaugg agacccugga gcggcuccag cgccgguacg     8220

gcgggggccu ggugcgcguc ccccuguccc ggaacagcac ccacgagaug uacugggugu     8280

ccggggccaa gagcaacacg aucaaguccg ugagcaccac cucccagcuc cugcugggcc     8340

gcauggacgg cccccggcgc ccggucaagu acgaggagga cgugaaccuc gggagcggca     8400

cccgggccgu ggucuccugc gccgaggccc ccaacaugaa gaucaucggg aaccgcaucg     8460

agcggauccg cagcgagcac gcggagacgu gguucuucga cgagaaccac cccuaccgga     8520

ccugggccua ccacggcucc uacgaggccc cgacccaggg gagcgccucc agccugauca     8580

acggcguggu gcgccugcuc uccaagcccu gggacgucgu gaccggcgug acggggaucg     8640

ccaugaccga caccaccccc uacggccagc agcgggucuu caaggagaag guggacacgc     8700

gcgugcccga cccgcaggag gggacccggc aggucaugag cauggugagc uccuggcugu     8760

ggaaggagcu gggcaagcac aagcgccccc gcgugugcac caaggaggag uucaucaaca     8820

agguccggag caacgcggcc cucggggcca ucuucgagga ggagaaggag uggaagaccg     8880

ccguggaggc cgugaacgac ccccgcuucu gggcgcuggu cgacaaggag cgggagcacc     8940

accuccgcgg cgagugccag uccugcgugu acaacaugau gggcaagcgg gagaagaagc     9000

agggggaguu cggcaaggcc aaggggagcc gcgccaucug guacaugugg cucggcgccc     9060

gguuccugga guucgaggcg cugggguucc ucaacgagga ccacuggaug ggccgcgaga     9120

acuccggcgg gggcguggag gggcugggcc ugcagcggcu cggguacguc cuggaggaga     9180

ugagccgcau ccccggcggc cggauguacg ccgacgacac ggccgggugg gacacccgca     9240

ucucccgguu cgaccuggag aacgaggccc ucaucaccaa ccagauggag aagggccacc     9300

gcgcccuggc gcuggccauc aucaaguaca ccuaccagaa caaggugguc aaggugcucc     9360

ggccggccga gaaggggaag acggugaugg acaucaucag ccgccaggac cagcggggcu     9420

ccggccaggu cgugaccuac gcccugaaca ccuucaccaa ccuggugguc cagcucaucc     9480

gcaacaugga ggccgaggag gugcuggaga ugcaggaccu guggcuccug cggcgcagcg     9540

agaaggugac caacuggcug caguccaacg ggugggaccg gcucaagcgc auggcgguca     9600

gcggcgacga cugcguggug aagcccaucg acgaccgcuu cgcccacgcc cugcgguucc     9660

ugaacgacau ggggaagguc cgcaaggaca cgcaggagug gaagcccucc accggcuggg     9720

acaacuggga ggaggugccc uucugcagcc accacuucaa caagcuccac cugaaggacg     9780

ggcgguccau cguggucccg ugccgccacc aggacgagcu gaucggccgg gcccgcguga     9840

gccccggcgc cggguggucc auccgggaga ccgcgugccu cgccaagagc uacgcccaga     9900

uguggcagcu gcuguacuuc caccgccggg accuccgccu gauggccaac gcgaucugcu     9960

ccagcgugcc cgucgacugg gugcccaccg gccggacgac cuggagcauc cacgggaagg    10020

gcgaguggau gaccaccgag gacaugcugg uggucuggaa ccgcgugugg aucgaggaga    10080

acgaccacau ggaggacaag acgccgguga ccaaguggac cgacaucccc uaccucggga    10140

agcgggagga ccuguggugc ggcucccuga ucggccaccg cccccggacc acgugggccg    10200

agaacaucaa gaacaccguc aacauggugc gccggaucau cggggacgag gagaaguaca    10260

uggacuaccu cagcacccag gugcgcuacc ugggcgagga gggguccacc cccggcgucc    10320

ugugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag    10380

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac    10440

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua    10500

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc    10620

ccccccaaag gcucuuuuca gagccaccag aauu                                10654


<210>  307
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 GC

<400>  307
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcucc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacacgug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

cgagcaccug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cgcgcaagcu gcagacccgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccacgagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg guggacgucg      720

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      780

agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac cuguacuacc     1260

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg     1380

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc caggaggggg     1440

ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acgcuccacg     1620

gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1680

agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg     1740

ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1920

ugggggacac ggcgugggac uucggcagcg ucggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacc cugcugaugu ggcucgggcu gaacgccaag aacggcucca     2100

ucagccugau gugccucgcg cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  308
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 GC

<400>  308
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacacgug cgacgccacc auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcacgug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacgcgc agccagaccu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggacgugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacg gacgagaacc     1140

gcgcgaaggu cgagaucacc ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac accggcaccc cgcacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac ggcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugacgc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg ccguucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac gcaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ugggcggcgc ccugaacucc cucgggaagg     1980

ggauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacg cuccugaugu ggcugggccu caacgccaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgucagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  309
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 GC

<400>  309
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      120

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg guggacgucg      180

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      240

agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      360

agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc      540

acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      660

gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc      780

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc caggaggggg      900

ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc      960

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1020

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acgcuccacg     1080

gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1140

agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg     1200

ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg caccggagcg     1320

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1380

ugggggacac ggcgugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  310
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 GC

<400>  310
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagccgacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu cuucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcug ggcggguucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaacccgg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  311
<211>  10642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein mRNA Design 2 GC

<400>  311
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

accccaagga ggagauccgc cggauccgca ucgugaacau gcugaagcgg ggcgucgccc      120

gcgugaaccc gcucgggggc cugaagcggc ugcccgccgg gcuccugcug ggccacgggc      180

ccauccgcau ggugcucgcg auccuggccu uccugcgguu caccgccauc aagcccagcc      240

ucggccugau caaccgcugg ggcuccgucg ggaagaagga ggccauggag aucaucaaga      300

aguucaagaa ggaccuggcc gcgaugcucc ggaucaucaa cgcccgcaag gagcggaagc      360

gccggggcgc cgacaccagc aucgggauca ucggccugcu gcucacgacc gccauggcgg      420

ccgagaucac ccgccggggg uccgccuacu acauguaccu ggaccgcagc gacgccggca      480

aggccaucuc cuucgcgacc acgcugggcg ugaacaagug ccacgugcag aucauggacc      540

ucgggcacau gugcgacgcc accaugagcu acgagugccc gaugcuggac gagggcgucg      600

agcccgacga cguggacugc uggugcaaca ccaccuccac gugggugguc uacgggaccu      660

gccaccacaa gaagggcgag gcccggcgca gccggcgcgc cgugacccug cccucccaca      720

gcacccggaa gcuccagacg cgcucccaga ccuggcugga gagccgcgag uacaccaagc      780

accugaucaa gguggagaac uggaucuucc ggaaccccgg cuucgcccuc gucgcggugg      840

ccaucgccug gcugcugggg uccagcaccu cccagaaggu gaucuaccuc gucaugaucc      900

ugcugaucgc cccggccuac agcauccgcu gcaucggcgu guccaaccgg gacuucgugg      960

aggggaugag cggcgggacg ugggucgacg uggugcucga gcacggcggc ugcgucaccg     1020

ugauggcgca ggacaagccc accguggaca ucgagcuggu caccacgacc guguccaaca     1080

uggccgaggu ccgcagcuac ugcuacgagg ccagcaucuc cgacauggcc agcgacuccc     1140

ggugccccac ccagggggag gccuaccugg acaagcagag cgacacccag uacgugugca     1200

agcgcacgcu cguggaccgg ggcuggggga acggcugcgg gcuguucggc aagggcuccc     1260

uggucacgug cgcgaaguuc acgugcagca agaagaugac cgggaagucc auccagcccg     1320

agaaccucga guaccgcauc augcugagcg ugcacggcuc ccagcacagc gggaugaucg     1380

gcuacgagac ggacgaggac cgggccaagg uggaggucac cccgaacucc ccgcgcgccg     1440

aggccacccu gggcggguuc ggcagccucg ggcuggacug cgagccccgg accggccugg     1500

acuucuccga ccucuacuac cugacgauga acaacaagca cuggcuggug cacaaggagu     1560

gguuccacga caucccccuc ccguggcacg cgggggccga caccggcacc ccgcacugga     1620

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagcgccag accgucgugg     1680

ugcugggcag ccaggagggg gcgguccaca cggcccuggc cggcgcccuc gaggccgaga     1740

uggacggggc gaagggccgg cuguucuccg ggcaccugaa gugccgccuc aagauggaca     1800

agcugcggcu gaagggcgug agcuacuccc ucugcaccgc cgccuucacc uucaccaagg     1860

ugcccgccga gacccugcac ggcacgguca ccguggaggu gcaguacgcc gggaccgacg     1920

gccccugcaa gaucccgguc cagauggcgg uggacaugca gacccugacg cccguggggc     1980

gccucaucac cgccaacccc gucaucaccg agagcaccga gaacuccaag augaugcugg     2040

agcuggaccc gccguucggc gacagcuaca ucgugaucgg ggugggcgac aagaagauca     2100

cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc accguccgcg     2160

gcgcgaagcg gauggccgug cucggggaca ccgccuggga cuucggcagc gugggcggcg     2220

ucuucaacuc ccuggggaag gggauccacc agaucuucgg cgccgccuuc aagagccugu     2280

ucgggggcau guccugguuc agccagaucc ucaucgggac gcugcuggug uggcucggcc     2340

ugaacaccaa gaacggcucc aucagccuga ccugccucgc gcugggcggc gugaugaucu     2400

uccuguccac cgccgucagc gccgacgugg ggugcuccgu ggacuucagc aagaaggaga     2460

cgcgcugcgg gaccggcguc uucaucuaca acgacgugga ggccuggcgc gaccgguaca     2520

aguaccaccc cgacuccccg cgccggcucg ccgcggccgu gaagcaggcc ugggaggagg     2580

ggaucugcgg caucagcucc gucagccgca uggagaacau cauguggaag uccguggagg     2640

gggagcugaa cgccauccug gaggagaacg gcgugcagcu caccgucgug guggggagcg     2700

ucaagaaccc cauguggcgg ggcccgcagc gccugcccgu gcccgugaac gagcugccgc     2760

acggcuggaa ggccuggggg aagagcuacu ucguccgggc ggccaagacc aacaacuccu     2820

ucguggugga cggcgacacg cucaaggagu gcccccugga gcaccgcgcc uggaacagcu     2880

uccuggucga ggaccacggg uucggcgugu uccacaccuc cguguggcuc aagguccggg     2940

aggacuacag ccuggagugc gaccccgccg ugaucgggac cgcggucaag ggccgcgagg     3000

ccgcccacuc cgaccugggc uacuggaucg agagcgagaa gaacgacacc uggcggcuca     3060

agcgcgccca ccugaucgag augaagacgu gcgaguggcc caagucccac acccugugga     3120

ccgacggggu ggaggagagc gaccucauca ucccgaaguc ccuggccggc ccccugagcc     3180

accacaacac ccgggagggg uaccgcaccc aggugaaggg ccccuggcac uccgaggagc     3240

ucgagauccg guucgaggag ugccccggga ccaaggucua cguggaggag accugcggca     3300

cccgcggccc gagccugcgg uccacgaccg cgagcgggcg cgugaucgag gaguggugcu     3360

gccgggagug caccaugccg ccccuguccu uccgcgccaa ggacggcugc ugguacggga     3420

uggagauccg gccccgcaag gagccggaga gcaaccucgu ccgcuccaug gugaccgccg     3480

gcagcacgga ccacauggac cacuucuccc ugggcgugcu ggucauccuc cugauggugc     3540

aggaggggcu gaagaagcgg augaccacca agaucaucau gagcaccagc auggccgugc     3600

ucgucgugau gauccugggc ggguucucca ugagcgaccu ggccaagcuc gugauccuga     3660

ugggcgcgac cuucgccgag augaacacgg gcggcgacgu cgcccaccug gcccucgugg     3720

ccgcguucaa ggugcgcccc gcccugcugg ucuccuucau cuuccgggcc aacuggaccc     3780

cgcgcgagag caugcuccug gcccuggccu ccugccuccu gcagaccgcg aucagcgccc     3840

uggaggggga ccucauggug cugaucaacg gguucgcccu ggccuggcuc gcgauccggg     3900

ccauggccgu gccccgcacc gacaacaucg cccugccgau ccuggccgcg cucacgcccc     3960

uggcccgggg cacccugcuc gucgccuggc gcgccgggcu ggccacgugc ggcgggauca     4020

ugcugcucuc ccugaagggc aagggcagcg ugaagaagaa ccugcccuuc gugauggcgc     4080

ucgggcugac cgccguccgg gugguggacc ccaucaacgu cgugggccug cuccugcuga     4140

cgcgcuccgg gaagcggagc uggccgcccu ccgaggugcu caccgccguc ggccugaucu     4200

gcgcccuggc cgggggcuuc gcgaaggccg acaucgagau ggccggcccc auggccgcgg     4260

uggggcuccu gaucgugagc uacgucgugu ccggcaagag cguggacaug uacaucgagc     4320

gcgccgggga caucaccugg gagaaggacg ccgaggucac cggcaacucc ccgcggcugg     4380

acguggcccu cgacgagagc ggggacuucu cccuggugga ggaggacggc ccgcccaugc     4440

gcgagaucau ccugaagguc gugcucaugg ccaucugcgg caugaacccc aucgcgaucc     4500

cguucgccgc cggggccugg uacguguacg ucaagacggg caagcggagc ggggcccugu     4560

gggacgugcc cgcgcccaag gaggugaaga agggcgagac caccgacggc gucuaccgcg     4620

ugaugacccg gcgccugcug ggguccaccc aggugggcgu cggggugaug caggagggcg     4680

uguuccacac cauguggcac gucaccaagg gggccgcccu ccggagcggc gagggccgcc     4740

uggaccccua cuggggcgac gugaagcagg accuggucag cuacugcggg ccguggaagc     4800

ucgacgccgc cugggacggg cuguccgagg ugcagcugcu cgcggugccg cccggcgagc     4860

gcgcccggaa cauccagacc cugcccggga ucuucaagac gaaggacggc gacaucggcg     4920

ccgucgcccu ggacuacccg gcggggacca gcggcucccc cauccucgac aagugcgggc     4980

gcgugaucgg ccuguacggg aacggcgugg ucaucaagaa cggcagcuac guguccgcca     5040

ucacccaggg gaagcgggag gaggagaccc ccguggagug cuucgagccc agcaugcuga     5100

agaagaagca gcucacgguc cuggaccugc acccgggcgc cgggaagacc cgccgggugc     5160

uccccgagau cgugcgcgag gccaucaaga agcggcugcg caccgucauc cuggccccca     5220

cccggguggu ggcggccgag auggaggagg cccuccgcgg ccugcccguc cgguacauga     5280

cgaccgccgu gaacgugacc cacuccggga ccgagaucgu cgaccugaug ugccacgcca     5340

cguucaccag ccgccuccug cagccgaucc gggugcccaa cuacaaccug aacaucaugg     5400

acgaggcgca cuucaccgac cccuccagca ucgccgcccg cggcuacauc uccacccggg     5460

uggagauggg cgaggccgcc gcgaucuuca ugacggccac cccgccgggg acccgcgacg     5520

ccuuccccga cagcaacucc cccaucaugg acaccgaggu cgaggugccc gagcgggccu     5580

ggagcuccgg cuucgacugg gugaccgacc acagcgggaa gacggucugg uucgugccgu     5640

ccgugcgcaa cggcaacgag aucgccgcgu gccucaccaa ggccggcaag cgggucaucc     5700

agcugagccg caagaccuuc gagaccgagu uccagaagac gaagaaccag gagugggacu     5760

ucgugaucac caccgacauc uccgagaugg gggccaacuu caaggccgac cgggugaucg     5820

acagccgccg cugccugaag cccgucaucc ucgacggcga gcgggugauc cuggcggggc     5880

ccaugcccgu gacccacgcc agcgccgccc agcgccgggg ccgcaucggg cggaacccga     5940

acaagcccgg cgacgaguac auguacggcg ggggcugcgc cgagacggac gaggggcacg     6000

cgcacuggcu ggaggcccgc augcuccugg acaacaucua ccugcaggac ggccucaucg     6060

ccucccugua ccggcccgag gccgacaagg ucgccgcgau cgagggggag uucaagcugc     6120

gcaccgagca gcggaagacc uucguggagc ucaugaagcg cggcgaccug ccgguguggc     6180

uggccuacca ggucgccagc gccggcauca ccuacacgga ccggcgcugg ugcuucgacg     6240

ggaccaccaa caacaccauc auggaggacu ccgugcccgc cgaggugugg acgaaguacg     6300

gcgagaagcg gguccucaag ccccgcugga uggacgcgcg ggugugcagc gaccacgccg     6360

cccugaaguc cuucaaggag uucgccgcgg ggaagcgcgg cgccgcccug ggggugaugg     6420

aggcccucgg cacccugccc ggccacauga ccgagcgguu ccaggaggcc aucgacaacc     6480

uggcgguccu caugcgcgcc gagaccggga gccggccgua caaggccgcc gccgcgcagc     6540

ugcccgagac gcuggagacc aucaugcucc ugggccugcu cgggaccgug ucccugggca     6600

ucuucuucgu gcugaugcgc aacaagggca ucgggaagau gggcuucggg auggucaccc     6660

ucggcgccag cgccuggcug auguggcugu ccgagaucga gcccgcccgc aucgccugcg     6720

ugcucaucgu ggucuuccug cugcucgugg ugcugauccc cgagccggag aagcagcgga     6780

gcccccagga caaccagaug gcgaucauca ucauggucgc cguggggcug cucggccuga     6840

ucacggccaa cgagcugggc uggcucgagc gcaccaagaa cgacaucgcc caccugaugg     6900

ggcggcgcga ggagggcgcg accauggggu ucuccaugga caucgaccug cggcccgcca     6960

gcgccugggc caucuacgcc gcgcucacca cgcugaucac ccccgccguc cagcacgccg     7020

ugaccaccuc cuacaacaac uacagccuga uggccauggc cacgcaggcg ggcgugcucu     7080

ucgggauggg caagggcaug ccguucaugc acggggaccu gggcgucccg cugcucauga     7140

uggggugcua cucccagcug accccgcuga cccucaucgu ggccaucauc cugcuggugg     7200

cccacuacau guaccucauc cccggccugc aggccgccgc ggcccgcgcc gcccagaagc     7260

ggaccgccgc ggggaucaug aagaacccgg ucguggacgg caucgugguc acggacaucg     7320

acaccaugac caucgacccc cagguggaga agaagauggg ccaggugcug cucaucgccg     7380

ucgccaucag cuccgccgug cugcugcgca ccgcgugggg guggggcgag gccggggccc     7440

ucaucacggc cgccaccagc acccuguggg agggcucccc caacaaguac uggaacagca     7500

gcaccgcgac cucccugugc aacaucuucc gggggagcua ccucgccggc gccucccuga     7560

ucuacacggu gacccgcaac gccggccugg ucaagcggcg cgggggcggg accggcgaga     7620

cccucggcga gaaguggaag gcccggcuga accagaugag cgcgcuggag uucuacuccu     7680

acaagaagag cgggaucacg gaggugugcc gcgaggaggc ccggcgcgcc cucaaggacg     7740

gcguggccac cgggggccac gccgucuccc gggggagcgc gaagauccgc uggcuggagg     7800

agcggggcua ccugcagccc uacggcaagg ugguggaccu ggggugcggc cgcgggggcu     7860

gguccuacua cgccgccacc auccggaagg uccaggaggu gcgcggguac accaagggcg     7920

gcccggggca cgaggagccc augcucgugc agagcuacgg cuggaacauc guccgccuga     7980

aguccggggu ggacguguuc cacauggccg cggagcccug cgacacgcug cucugcgaca     8040

ucggcgagag cuccagcucc cccgaggucg aggagacccg gacccugcgc gugcugagca     8100

ugguggggga cuggcucgag aagcggccgg gcgccuucug caucaagguc cugugccccu     8160

acaccuccac gaugauggag accauggagc gccugcagcg gcgccacggc gggggccucg     8220

ugcgggugcc ccugugccgc aacagcaccc acgagaugua cugggucucc ggggccaaga     8280

gcaacaucau caagagcgug uccaccacga gccagcugcu ccugggccgg auggacggcc     8340

cccgccggcc ggugaaguac gaggaggacg ucaaccuggg guccggcacc cgcgccgugg     8400

ccagcugcgc ggaggccccc aacaugaaga ucaucgggcg gcgcaucgag cggauccgca     8460

acgagcacgc cgagaccugg uuccucgacg agaaccaccc cuaccggacc ugggccuacc     8520

acggcuccua cgaggccccg acgcagggga gcgcguccag ccuggugaac ggcgucgugc     8580

gccugcucuc caagcccugg gacgugguca ccggcgugac cgggaucgcc augaccgaca     8640

cgacccccua cggccagcag cggguguuca aggagaaggu cgacacccgc gugcccgacc     8700

cgcaggaggg gacccgccag gugaugaaca ucgucagcuc cuggcugugg aaggagcugg     8760

gcaagcggaa gcgcccccgg gugugcacga aggaggaguu caucaacaag gugcgcagca     8820

acgccgcccu cggggccauc uucgaggagg agaaggagug gaagaccgcg gucgaggccg     8880

ugaacgaccc ccgguucugg gcccuggucg accgcgagcg ggagcaccac cuccgcggcg     8940

agugccacuc cugcguguac aacaugaugg gcaagcggga gaagaagcag ggggaguucg     9000

gcaaggccaa ggggagccgc gccaucuggu acauguggcu cggcgcgcgg uuccuggagu     9060

ucgaggcccu gggguuccuc aacgaggacc acuggauggg ccgcgagaac uccggcgggg     9120

gcguggaggg gcugggccug cagcggcucg gguacauccu ggaggagaug aaccgcgccc     9180

ccggcggcaa gauguacgcc gacgacaccg cgggguggga cacccggauc agcaaguucg     9240

accuggagaa cgaggcccuc aucacgaacc agauggagga gggccaccgc acccuggccc     9300

uggccgucau caaguacacc uaccagaaca agguggugaa gguccuccgg ccggccgagg     9360

gcgggaagac cgugauggac aucaucuccc gccaggacca gcggggcagc ggccaggugg     9420

ucacguacgc gcugaacacc uucaccaacc ugguggugca gcucauccgc aacauggagg     9480

ccgaggaggu ccuggagaug caggaccugu ggcuccugcg gaagcccgag aaggugaccc     9540

gcuggcugca gagcaacggc ugggaccgcc ucaagcggau ggccgugucc ggggacgacu     9600

gcgucgugaa gcccaucgac gaccgcuucg cccacgcccu gcgguuccug aacgacaugg     9660

gcaaggugcg caaggacacc caggagugga agcccagcac gggguggucc aacugggagg     9720

aggucccguu cugcagccac cacuucaaca agcucuaccu gaaggacggc cgguccaucg     9780

uggugcccug ccgccaccag gacgagcuga ucggccgggc gcgcgucagc cccggggccg     9840

gcugguccau ccgggagacc gccugccucg ccaagagcua cgcccagaug uggcagcugc     9900

uguacuucca ccgccgggac cuccgccuga uggcgaacgc caucugcucc gccgugcccg     9960

uggacugggu cccgaccggg cggaccacgu ggagcaucca cggcaagggg gaguggauga    10020

ccaccgagga caugcugaug guguggaacc gcguguggau cgaggagaac gaccacaugg    10080

aggacaagac ccccgucacg aaguggaccg acauccccua ccucggcaag cgggaggacc    10140

uguggugcgg cucccugauc gggcaccgcc cccggaccac cugggccgag aacaucaagg    10200

acacggugaa cauggugcgc cggaucaucg gcgacgagga gaaguacaug gacuaccuca    10260

gcacccaggu ccgcuaccug ggggaggagg gcuccacccc gggggugcug ugaggacuag    10320

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc    10380

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa    10440

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga    10500

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc    10620

ucuuuucaga gccaccagaa uu                                             10642


<210>  312
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 GC

<400>  312
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag cggaagcgcc ggggcgccga caccagcauc gggaucaucg      120

gccugcugcu cacgaccgcc auggcggccg agaucacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcaagg ccaucuccuu cgcgaccacg cugggcguga      240

acaagugcca cgugcagauc auggaccucg ggcacaugug cgacgccacc augagcuacg      300

agugcccgau gcuggacgag ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccacgug gguggucuac gggaccugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu gacccugccc ucccacagca cccggaagcu ccagacgcgc ucccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugaucaaggu ggagaacugg aucuuccgga      540

accccggcuu cgcccucguc gcgguggcca ucgccuggcu gcuggggucc agcaccuccc      600

agaaggugau cuaccucguc augauccugc ugaucgcccc ggccuacagc auccgcugca      660

ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg gucgacgugg      720

ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc uacgaggcca      840

gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga     1020

agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg gccaaggugg     1140

aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc agccucgggc     1200

uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug acgaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg uggcacgcgg     1320

gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg guccacacgg     1440

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug uucuccgggc     1500

accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc uacucccucu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc acggucaccg     1620

uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag auggcggugg     1680

acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc aucaccgaga     1740

gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac agcuacaucg     1800

ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1860

ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc ggggacaccg     1920

ccugggacuu cggcagcgug ggcggcgucu ucaacucccu ggggaagggg auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc cagauccuca     2040

ucgggacgcu gcuggugugg cucggccuga acaccaagaa cggcuccauc agccugaccu     2100

gccucgcgcu gggcggcgug augaucuucc uguccaccgc cgucagcgcc gacguggggu     2160

gcuccgugga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  313
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 GC

<400>  313
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc cggacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu gacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgga      540

accccggguu cgcccuggug gccguggcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggucau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccgcugca      660

ucgggguguc caaccgggac uucgucgagg gcaugagcgg cgggaccugg guggacgugg      720

uccuggagca cggcgggugc gugacgguga uggcccagga caagccgacc gucgacaucg      780

agcuggugac caccacggug uccaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ucacgugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucggcu acgagaccga cgaggaccgc gccaaggucg     1140

aggugacgcc caacuccccg cgggccgagg cgacccucgg gggcuucggg agccugggcc     1200

uggacugcga gccccgcacc ggccucgacu ucuccgaccu guacuaccug accaugaaca     1260

acaagcacug gcucgugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gggccgacac gggcaccccg cacuggaaca acaaggaggc ccuggucgag uucaaggacg     1380

cccacgcgaa gcggcagacc gugguggucc ucgggagcca ggagggcgcc gugcacaccg     1440

cccuggccgg ggcgcuggag gccgagaugg acggcgccaa gggccgccuc uucuccgggc     1500

accugaagug ccggcugaag auggacaagc uccgccugaa gggcgugagc uacucccugu     1560

gcacggccgc cuucaccuuc accaaggucc ccgcggagac ccuccacggg accgugacgg     1620

uggaggucca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1680

acaugcagac ccugaccccg gucgggcggc ugaucacggc caacccggug aucaccgaga     1740

gcaccgagaa cuccaagaug augcucgagc uggacccgcc cuucggcgac agcuacaucg     1800

ugaucggggu cggcgacaag aagaucaccc accacuggca ccgcuccggg agcacgaucg     1860

gcaaggccuu cgaggcgacc gugcggggcg ccaagcgcau ggccgugcug ggggacaccg     1920

ccugggacuu cggcagcguc gggggcgugu ucaacucccu ggggaagggc auccaccaga     1980

ucuucggcgc cgcguucaag agccucuucg ggggcauguc cugguucagc cagauccuga     2040

ucgggacccu gcucgugugg cugggccuga acacgaagaa cggguccauc agccucaccu     2100

gccuggcccu gggcggcguc augaucuucc ucuccaccgc cgugagcgcc gacguggggu     2160

gcuccgucga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  314
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 GC

<400>  314
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggugau cuaccucguc augauccugc ugaucgcccc ggccuacagc auccgcugca      120

ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg gucgacgugg      180

ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc guggacaucg      240

agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga      480

agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgugc      540

acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg gccaaggugg      600

aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc agccucgggc      660

uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug acgaugaaca      720

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg uggcacgcgg      780

gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag uucaaggacg      840

cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg guccacacgg      900

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug uucuccgggc      960

accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc uacucccucu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc acggucaccg     1080

uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag auggcggugg     1140

acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc aucaccgaga     1200

gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac agcuacaucg     1260

ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1320

ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc ggggacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  315
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 GC

<400>  315
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagccgacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu cuucggcaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucggcu acgagaccga cgaggaccgg gccaaggugg      600

aggugacccc caacuccccg cgcgccgagg ccacgcuggg cggguucggc agccuggggc      660

ucgacugcga gccccggacc ggccuggacu ucuccgaccu guacuaccuc accaugaaca      720

acaagcacug gcugguccac aaggaguggu uccacgacau cccccugccc uggcacgcgg      780

gggccgacac cggcacgccg cacuggaaca acaaggaggc ccucguggag uucaaggacg      840

cccacgccaa gcgccagacc guggucgugc uggggagcca ggagggcgcg gugcacaccg      900

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug uucagcgggc      960

accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgucucc uacagccucu     1020

gcaccgccgc cuucacguuc accaaggugc ccgccgagac ccugcacggg accgucacgg     1080

uggaggugca guacgccggc accgacggcc ccugcaagau ccccguccag auggcggugg     1140

acaugcagac ccugaccccg guggggcgcc ucaucacggc caacccgguc aucaccgagu     1200

ccaccgagaa cagcaagaug augcuggagc uggacccgcc cuucggcgac uccuacaucg     1260

ugaucggggu gggcgacaag aagaucaccc accacuggca ccggagcggg uccacgaucg     1320

gcaaggccuu cgaggccacc guccgcggcg ccaagcggau ggcggugcuc ggggacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  316
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 
       opimized

<400>  316
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccggaaagag aagaagcgca gaggcgccga uaccagcgug ggcauugugg      120

gccugcugcu gacaacagcc auggccgccg aagugaccag aagaggcagc gccuacuaca      180

uguaccugga ccggaacgau gccggcgagg ccaucagcuu uccaaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggaac ccgacgaugu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccuguc accacaagaa gggcgaagcc agacggucca      420

gacgggccgu gacacugccu agccacagca ccagaaagcu gcagacccgg ucccagaccu      480

ggcuggaaag cagagaguac accaagcacc ugauccgggu ggaaaacugg aucuuccgga      540

accccggcuu ugcccuggcc gcugcugcua uugcuuggcu gcugggcagc agcaccuccc      600

agaaagugau cuaccucgug augauccugc ugaucgcccc ugccuacagc auccggugua      660

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacaucg      720

ugcuggaaca cggcggcugc gugacaguga uggcccagga uaagcccacc guggauaucg      780

agcucgugac caccaccgug uccaauaugg ccgaagugcg gagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gcccuacaca gggcgaggcc uaccuggaca      900

agcaguccga cacccaguac gugugcaagc ggacccuggu ggauagaggc uggggcaaug      960

gcugcggccu guuuggcaag ggcagccucg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc     1080

acggcagcca gcacuccggc augaucguga acgacaccgg ccacgagaca gacgagaacc     1140

gggccaaggu ggaaaucacc cccaacagcc cuagagccga ggccacacug ggcggcuuug     1200

gaucucuggg ccuggacugc gagccuagaa ccggccugga uuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac aucccccugc     1320

ccuggcaugc cggcgcugau acaggcacac cccacuggaa caacaaagag gcucuggugg     1380

aguucaagga cgcccacgcc aagaggcaga ccgugguggu gcugggaucu caggaaggcg     1440

ccgugcauac agcucuggcu ggcgcccugg aagccgaaau ggauggcgcu aagggcagac     1500

uguccagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug aagggcgugu     1560

ccuacagccu guguaccgcc gccuucaccu ucaccaagau ccccgccgag acacugcacg     1620

gcaccgugac uguggaagug caguacgccg gcaccgacgg cccuuguaaa gugccugcuc     1680

agauggcugu ggacaugcag acccugaccc cugugggcag gcugaucacc gccaacccug     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca cccuucggcg     1800

acagcuacau cgugaucggc gugggggaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uuugaggcua cagugcgggg agccaagaga auggccgugc     1920

ugggagauac cgccugggac uuuggcucug ugggcggagc ccugaacucu cugggcaagg     1980

gaauccacca gaucuucgga gccgccuuua agagccuguu cggcggcaug uccugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucucccugau gugccuggcu cugggaggcg ugcugaucuu ccugagcaca gccgugucug     2160

ccgacguggg cugcagcgug gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  317
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 
       opimized

<400>  317
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaagugau cuaccucgug augauccugc ugaucgcccc ugccuacagc auccggugua      120

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacaucg      180

ugcuggaaca cggcggcugc gugacaguga uggcccagga uaagcccacc guggauaucg      240

agcucgugac caccaccgug uccaauaugg ccgaagugcg gagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gcccuacaca gggcgaggcc uaccuggaca      360

agcaguccga cacccaguac gugugcaagc ggacccuggu ggauagaggc uggggcaaug      420

gcugcggccu guuuggcaag ggcagccucg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc      540

acggcagcca gcacuccggc augaucguga acgacaccgg ccacgagaca gacgagaacc      600

gggccaaggu ggaaaucacc cccaacagcc cuagagccga ggccacacug ggcggcuuug      660

gaucucuggg ccuggacugc gagccuagaa ccggccugga uuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac aucccccugc      780

ccuggcaugc cggcgcugau acaggcacac cccacuggaa caacaaagag gcucuggugg      840

aguucaagga cgcccacgcc aagaggcaga ccgugguggu gcugggaucu caggaaggcg      900

ccgugcauac agcucuggcu ggcgcccugg aagccgaaau ggauggcgcu aagggcagac      960

uguccagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug aagggcgugu     1020

ccuacagccu guguaccgcc gccuucaccu ucaccaagau ccccgccgag acacugcacg     1080

gcaccgugac uguggaagug caguacgccg gcaccgacgg cccuuguaaa gugccugcuc     1140

agauggcugu ggacaugcag acccugaccc cugugggcag gcugaucacc gccaacccug     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca cccuucggcg     1260

acagcuacau cgugaucggc gugggggaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uuugaggcua cagugcgggg agccaagaga auggccgugc     1380

ugggagauac cgccugggac uuuggcucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  318
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 
       opimized

<400>  318
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccggaaagag aagaagcgca gaggcgccga uaccagcgug ggcauugugg      120

gccugcugcu gacaacagcc auggccgccg aagugaccag aagaggcagc gccuacuaca      180

uguaccugga ccggaacgau gccggcgagg ccaucagcuu uccaaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaccug ugacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggaac ccgacgaugu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccuguc accacaagaa gggcgaagcc agacggucca      420

gacgggccgu gacacugccu agccacagca ccagaaagcu gcagacccgg ucccagaccu      480

ggcuggaaag cagagaguac accaagcacc ugauccgggu ggaaaacugg aucuuccgga      540

accccggcuu ugcccuggcc gcugcugcua uugcuuggcu gcugggcagc agcaccuccc      600

agaaagugau cuaccucgug augauccugc ugaucgcccc ugccuacagc auccggugua      660

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacgugg      720

ugcuggaaca uggcggcugc gugacaguga uggcucagga caagcccacc guggacaucg      780

agcucgugac caccaccgug uccaauaugg ccgaagugcg gagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gcccuacaca gggcgaggcc uaccuggaca      900

agcaguccga cacccaguac gugugcaagc ggacccuggu ggauagaggc uggggcaaug      960

gcugcggccu guuuggcaag ggcagccucg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc     1080

acggcagcca gcacuccggc augaucguga acgacaccgg ccacgagaca gacgagaacc     1140

gggccaaggu ggaaaucacc cccaacagcc cuagagccga ggccacacug ggcggcuuug     1200

gaucucuggg ccuggacugc gagccuagaa ccggccugga uuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac aucccccugc     1320

ccuggcaugc cggcgcugau acaggcacac cccacuggaa caacaaagag gcucuggugg     1380

aguucaagga cgcccacgcc aagaggcaga ccgugguggu gcugggaucu caggaaggcg     1440

ccgugcauac agcucuggcu ggcgcccugg aagccgaaau ggauggcgcu aagggcagac     1500

uguccagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug aagggcgugu     1560

ccuacagccu guguaccgcc gccuucaccu ucaccaagau ccccgccgag acacugcacg     1620

gcaccgugac uguggaagug caguacgccg gcaccgacgg cccuuguaaa gugccugcuc     1680

agauggccgu ggauaugcag acccugaccc cugugggcag gcugaucacc gccaacccug     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca cccuucggcg     1800

acagcuacau cgugaucggc gugggggaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uuugaggcua cagugcgggg agccaagaga auggccgugc     1920

ugggagauac cgccugggac uuuggcucug ugggcggagc ccugaacucu cugggcaagg     1980

gaauccacca gaucuucgga gccgccuuua agagccuguu cggcggcaug uccugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag aacggcagca     2100

ucucccugau gugccuggcu cugggaggcg ugcugaucuu ccugagcaca gccgugucug     2160

ccgacguggg cugcagcgug gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  319
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 
       opimized

<400>  319
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaagugau cuaccucgug augauccugc ugaucgcccc ugccuacagc auccggugua      120

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacgugg      180

ugcuggaaca uggcggcugc gugacaguga uggcucagga caagcccacc guggacaucg      240

agcucgugac caccaccgug uccaauaugg ccgaagugcg gagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gcccuacaca gggcgaggcc uaccuggaca      360

agcaguccga cacccaguac gugugcaagc ggacccuggu ggauagaggc uggggcaaug      420

gcugcggccu guuuggcaag ggcagccucg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc      540

acggcagcca gcacuccggc augaucguga acgacaccgg ccacgagaca gacgagaacc      600

gggccaaggu ggaaaucacc cccaacagcc cuagagccga ggccacacug ggcggcuuug      660

gaucucuggg ccuggacugc gagccuagaa ccggccugga uuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac aucccccugc      780

ccuggcaugc cggcgcugau acaggcacac cccacuggaa caacaaagag gcucuggugg      840

aguucaagga cgcccacgcc aagaggcaga ccgugguggu gcugggaucu caggaaggcg      900

ccgugcauac agcucuggcu ggcgcccugg aagccgaaau ggauggcgcu aagggcagac      960

uguccagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug aagggcgugu     1020

ccuacagccu guguaccgcc gccuucaccu ucaccaagau ccccgccgag acacugcacg     1080

gcaccgugac uguggaagug caguacgccg gcaccgacgg cccuuguaaa gugccugcuc     1140

agauggccgu ggauaugcag acccugaccc cugugggcag gcugaucacc gccaacccug     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca cccuucggcg     1260

acagcuacau cgugaucggc gugggggaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uuugaggcua cagugcgggg agccaagaga auggccgugc     1380

ugggagauac cgccugggac uuuggcucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  320
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opimized

<400>  320
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccggaaagag cggaagcgga gaggcgccga uaccagcauc ggcaucaucg      120

gccugcugcu gaccacagcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu ugccacaacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggaac ccgacgaugu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccuguc accacaagaa gggcgaggcc agacggucua      420

gaagggccgu gacacugccu agccacagca cccggaagcu gcagaccaga agccagaccu      480

ggcuggaaag cagagaguac accaagcacc ugaucaaggu ggaaaacugg aucuuccgga      540

accccggcuu cgcccuggug gccguggcua uugcuuggcu gcugggcagc uccaccuccc      600

agaaagugau cuaccucgug augauccugc ugaucgcccc ugccuacagc auccggugua      660

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacgugg      720

ugcuggaaca uggcggcugc gugacaguga uggcccagga caagcccacc guggacaucg      780

agcucgugac caccaccgug uccaauaugg ccgaagugcg gagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gcccuacaca gggggaggcc uaccuggaua      900

agcaguccga cacccaguac gugugcaagc ggacccuggu ggauagaggc uggggcaaug      960

gcugcggccu guuuggcaag ggcagccucg ugaccugcgc caaguucacc uguagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc     1080

acggcagcca gcacagcgga augaucggcu acgagacaga cgaggaccgg gccaaagugg     1140

aagugacccc caacagcccu agagccgagg ccacacuggg cggcuuugga ucucugggcc     1200

uggacugcga gccuagaacc ggccuggauu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaagaguggu uccacgacau cccccugccc uggcaugccg     1320

gcgcugauac aggcacaccc cacuggaaca acaaagaggc ucugguggag uucaaggacg     1380

cccacgccaa gagacagacc gugguggugc ugggcucuca ggaaggcgcc gugcauacag     1440

cucuggcugg cgcccuggaa gccgaaaugg auggcgccaa gggcagacug uucagcggcc     1500

accugaagug ccggcugaag auggacaagc ugcggcugaa gggcgugucc uacagccugu     1560

guaccgccgc cuucaccuuc accaaggugc ccgccgaaac ccugcacggc acagugacug     1620

uggaagugca guacgccggc accgacggcc cuugcaagau cccugugcag auggccgugg     1680

auaugcagac ccugaccccu gugggcaggc ugaucaccgc caacccugug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggaac uggacccacc cuucggcgac agcuacaucg     1800

ugaucggcgu gggagacaag aagaucaccc accacuggca ccgcagcggc agcacaaucg     1860

gaaaggccuu cgaagccaca gugcggggag ccaagagaau ggccgugcug ggagauaccg     1920

ccugggacuu uggcucugug ggcggcgugu ucaacucccu gggcaaggga auccaccaga     1980

ucuucggagc cgccuuuaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcucgugugg cugggccuga acaccaagaa cggcagcauc ucccugaccu     2100

gccuggcucu gggcggagug augaucuucc ugagcaccgc cguguccgcc gaugugggcu     2160

guagcgugga cuucagcaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  321
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opimized

<400>  321
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaagugau cuaccucgug augauccugc ugaucgcccc ugccuacagc auccggugua      120

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacgugg      180

ugcuggaaca uggcggcugc gugacaguga uggcccagga caagcccacc guggacaucg      240

agcucgugac caccaccgug uccaauaugg ccgaagugcg gagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gcccuacaca gggggaggcc uaccuggaua      360

agcaguccga cacccaguac gugugcaagc ggacccuggu ggauagaggc uggggcaaug      420

gcugcggccu guuuggcaag ggcagccucg ugaccugcgc caaguucacc uguagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc      540

acggcagcca gcacagcgga augaucggcu acgagacaga cgaggaccgg gccaaagugg      600

aagugacccc caacagcccu agagccgagg ccacacuggg cggcuuugga ucucugggcc      660

uggacugcga gccuagaacc ggccuggauu ucagcgaccu guacuaccug accaugaaca      720

acaagcacug gcuggugcac aaagaguggu uccacgacau cccccugccc uggcaugccg      780

gcgcugauac aggcacaccc cacuggaaca acaaagaggc ucugguggag uucaaggacg      840

cccacgccaa gagacagacc gugguggugc ugggcucuca ggaaggcgcc gugcauacag      900

cucuggcugg cgcccuggaa gccgaaaugg auggcgccaa gggcagacug uucagcggcc      960

accugaagug ccggcugaag auggacaagc ugcggcugaa gggcgugucc uacagccugu     1020

guaccgccgc cuucaccuuc accaaggugc ccgccgaaac ccugcacggc acagugacug     1080

uggaagugca guacgccggc accgacggcc cuugcaagau cccugugcag auggccgugg     1140

auaugcagac ccugaccccu gugggcaggc ugaucaccgc caacccugug aucaccgaga     1200

gcaccgagaa cagcaagaug augcuggaac uggacccacc cuucggcgac agcuacaucg     1260

ugaucggcgu gggagacaag aagaucaccc accacuggca ccgcagcggc agcacaaucg     1320

gaaaggccuu cgaagccaca gugcggggag ccaagagaau ggccgugcug ggagauaccg     1380

ccugggacuu uggcucuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  322
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 
       opimized

<400>  322
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccggaaagag aagaagcgca gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gacaaccgcc auggccgccg aagugaccag acggggcagc gccuacuaca      180

uguaccugga ccggaacgac gccggcgagg ccaucagcuu ucccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggaac ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccuguc accacaagaa gggcgaggcc agacggucca      420

gacgggccgu gacacugccc agccacagca cccggaagcu gcagaccaga agccagaccu      480

ggcuggaaag cagagaguac accaagcacc ugauccgggu ggaaaacugg aucuuccgga      540

accccggcuu cgcccuggcc gcugccgcua uugcuuggcu gcugggcagc agcaccagcc      600

agaaagugau cuaccuggug augauccugc ugaucgcccc ugccuacagc auccggugca      660

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacaucg      720

ugcuggaaca cggcggcugc gugaccguga uggcccagga caagcccacc guggauaucg      780

agcuggugac aaccaccgug uccaacaugg ccgaagugcg gagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggggaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc ggacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guuuggcaag ggcagccugg ugacaugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagaca gacgagaacc     1140

gggccaaggu ggaaaucacc cccaacagcc ccagagccga ggccacacug ggcggcuuug     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac aucccccugc     1320

ccuggcacgc uggcgccgau acaggcaccc cucacuggaa caacaaagag gcccuggucg     1380

aguucaagga cgcccacgcc aagcggcaga ccgugguggu gcugggaucu caggaaggcg     1440

ccguccacac agcccuggcu ggcgcccugg aagccgagau ggauggcgcc aagggcaggc     1500

ugagcagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug aagggcgugu     1560

ccuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acacugcacg     1620

gcaccgugac cguggaagug caguacgccg gcaccgacgg cccuuguaag gugccagccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggccg gcugaucacc gccaacccug     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca cagugcgggg agccaagaga auggccgugc     1920

ugggcgacac cgccugggac uuuggaucug ugggcggagc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucgga gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

uuagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg cugcagcgug gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  323
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 
       opimized

<400>  323
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaagugau cuaccuggug augauccugc ugaucgcccc ugccuacagc auccggugca      120

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacaucg      180

ugcuggaaca cggcggcugc gugaccguga uggcccagga caagcccacc guggauaucg      240

agcuggugac aaccaccgug uccaacaugg ccgaagugcg gagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggggaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc ggacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guuuggcaag ggcagccugg ugacaugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagaca gacgagaacc      600

gggccaaggu ggaaaucacc cccaacagcc ccagagccga ggccacacug ggcggcuuug      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac aucccccugc      780

ccuggcacgc uggcgccgau acaggcaccc cucacuggaa caacaaagag gcccuggucg      840

aguucaagga cgcccacgcc aagcggcaga ccgugguggu gcugggaucu caggaaggcg      900

ccguccacac agcccuggcu ggcgcccugg aagccgagau ggauggcgcc aagggcaggc      960

ugagcagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug aagggcgugu     1020

ccuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acacugcacg     1080

gcaccgugac cguggaagug caguacgccg gcaccgacgg cccuuguaag gugccagccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggccg gcugaucacc gccaacccug     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca cagugcgggg agccaagaga auggccgugc     1380

ugggcgacac cgccugggac uuuggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  324
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 
       opimized

<400>  324
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccggaaagag aagaagcgca gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gacaaccgcc auggccgccg aagugaccag acggggcagc gccuacuaca      180

uguaccugga ccggaacgac gccggcgagg ccaucagcuu ucccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaccug ugacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggaac ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccuguc accacaagaa gggcgaggcc agacggucca      420

gacgggccgu gacacugccc agccacagca cccggaagcu gcagaccaga agccagaccu      480

ggcuggaaag cagagaguac accaagcacc ugauccgggu ggaaaacugg aucuuccgga      540

accccggcuu cgcccuggcc gcugccgcua uugcuuggcu gcugggcagc agcaccagcc      600

agaaagugau cuaccuggug augauccugc ugaucgcccc ugccuacagc auccggugca      660

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacgugg      720

ugcuggaaca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac aaccaccgug uccaacaugg ccgaagugcg gagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggggaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc ggacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guuuggcaag ggcagccugg ugacaugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagaca gacgagaacc     1140

gggccaaggu ggaaaucacc cccaacagcc ccagagccga ggccacacug ggcggcuuug     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac aucccccugc     1320

ccuggcacgc uggcgccgau acaggcaccc cucacuggaa caacaaagag gcccuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgugguggu gcugggaucu caggaaggcg     1440

ccgugcacac agcccuggcu ggcgcccugg aagccgagau ggauggcgcc aagggcaggc     1500

ugagcagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug aagggcgugu     1560

ccuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acacugcacg     1620

gcaccgugac cguggaagug caguacgccg gcaccgacgg cccuugcaag gugccagccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggccg gcugaucacc gccaacccug     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca cagugcgggg agccaagaga auggccgugc     1920

ugggcgacac cgccugggac uuuggaucug ugggcggagc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucgga gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg cugcagcgug gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  325
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 
       opimized

<400>  325
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaagugau cuaccuggug augauccugc ugaucgcccc ugccuacagc auccggugca      120

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacgugg      180

ugcuggaaca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac aaccaccgug uccaacaugg ccgaagugcg gagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggggaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc ggacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guuuggcaag ggcagccugg ugacaugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagaca gacgagaacc      600

gggccaaggu ggaaaucacc cccaacagcc ccagagccga ggccacacug ggcggcuuug      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaagagug guuccacgac aucccccugc      780

ccuggcacgc uggcgccgau acaggcaccc cucacuggaa caacaaagag gcccuggugg      840

aguucaagga cgcccacgcc aagcggcaga ccgugguggu gcugggaucu caggaaggcg      900

ccgugcacac agcccuggcu ggcgcccugg aagccgagau ggauggcgcc aagggcaggc      960

ugagcagcgg ccaccugaag ugccggcuga agauggacaa gcugcggcug aagggcgugu     1020

ccuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acacugcacg     1080

gcaccgugac cguggaagug caguacgccg gcaccgacgg cccuugcaag gugccagccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggccg gcugaucacc gccaacccug     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga acuggaccca cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca cagugcgggg agccaagaga auggccgugc     1380

ugggcgacac cgccugggac uuuggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  326
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opimized

<400>  326
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccggaaagag cggaagcgga gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag acggggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggaac ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccuguc accacaagaa gggcgaggcc agacggucca      420

gacgggccgu gacacugccc agccacagca cccggaagcu gcagaccaga agccagaccu      480

ggcuggaaag cagagaguac accaagcacc ugaucaaggu ggaaaacugg aucuuccgga      540

accccggcuu cgcccuggug gccguggcua uugccuggcu gcugggcagc agcaccagcc      600

agaaagugau cuaccuggug augauccugc ugaucgcccc ugccuacagc auccggugca      660

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacgugg      720

ugcuggaaca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac aaccaccgug uccaacaugg ccgaagugcg gagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggggaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc ggacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guuuggcaag ggcagccugg ugacaugcgc caaguucacc uguagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucggcu acgagacaga cgaggaccgg gccaaggugg     1140

aagugacccc caacagcccu agagccgagg ccacacuggg cggcuuuggc agccugggcc     1200

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaagaguggu uccacgacau cccccugccc uggcacgcug     1320

gcgccgauac aggcaccccu cacuggaaca acaaagaggc ccugguggag uucaaggacg     1380

cccacgccaa gcggcagacc gugguggugc ugggaucuca ggaaggcgcc gugcacacag     1440

cccuggcugg cgcccuggaa gccgagaugg auggcgccaa gggccggcug uucagcggcc     1500

accugaagug ccggcugaag auggacaagc ugcggcugaa gggcgugucc uacagccugu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac acugcacggc accgugaccg     1620

uggaagugca guacgccggc accgacggcc ccugcaagau cccugugcag auggccgugg     1680

acaugcagac ccugaccccc gugggccggc ugaucaccgc caacccugug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggaac uggacccacc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1860

gcaaggccuu cgaggccaca gugcggggag ccaagagaau ggccgugcug ggcgacaccg     1920

ccugggauuu uggcuccgug ggcggcgugu ucaacagccu gggcaagggc auccaccaga     1980

ucuucggagc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcuggucugg cugggccuga acaccaagaa cggcagcauc agccugaccu     2100

gccuggcccu gggcggagug augaucuucc ugagcaccgc cgugucugcc gacgugggcu     2160

gcagcgugga cuucuccaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  327
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opimized

<400>  327
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaagugau cuaccuggug augauccugc ugaucgcccc ugccuacagc auccggugca      120

ucggcguguc caaccgggac uucguggaag gcaugagcgg cggcacaugg guggacgugg      180

ugcuggaaca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac aaccaccgug uccaacaugg ccgaagugcg gagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggggaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc ggacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guuuggcaag ggcagccugg ugacaugcgc caaguucacc uguagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggaaua ccggaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucggcu acgagacaga cgaggaccgg gccaaggugg      600

aagugacccc caacagcccu agagccgagg ccacacuggg cggcuuuggc agccugggcc      660

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca      720

acaagcacug gcuggugcac aaagaguggu uccacgacau cccccugccc uggcacgcug      780

gcgccgauac aggcaccccu cacuggaaca acaaagaggc ccugguggag uucaaggacg      840

cccacgccaa gcggcagacc gugguggugc ugggaucuca ggaaggcgcc gugcacacag      900

cccuggcugg cgcccuggaa gccgagaugg auggcgccaa gggccggcug uucagcggcc      960

accugaagug ccggcugaag auggacaagc ugcggcugaa gggcgugucc uacagccugu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac acugcacggc accgugaccg     1080

uggaagugca guacgccggc accgacggcc ccugcaagau cccugugcag auggccgugg     1140

acaugcagac ccugaccccc gugggccggc ugaucaccgc caacccugug aucaccgaga     1200

gcaccgagaa cagcaagaug augcuggaac uggacccacc cuucggcgac agcuacaucg     1260

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1320

gcaaggccuu cgaggccaca gugcggggag ccaagagaau ggccgugcug ggcgacaccg     1380

ccugggauuu uggcuccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  328
<211>  10654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) complete polyprotein mRNA Design 2 
       optimized

<400>  328
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

accccaagaa gaagagcggc ggcuuccgca ucgugaacau gcugaagcgc ggcguggccc      120

gcgugagccc cuucggcggc cugaagcgcc ugcccgccgg ccugcugcug ggccacggcc      180

ccauccgcau ggugcuggcc auccuggccu uccugcgcuu caccgccauc aagcccagcc      240

ugggccugau caaccgcugg ggcagcgugg gcaagaagga ggccauggag aucaucaaga      300

aguucaagaa ggaccuggcc gccaugcugc gcaucaucaa cgcccgcaag gagaagaagc      360

gccgcggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc gccauggccg      420

ccgaggugac ccgccgcggc agcgccuacu acauguaccu ggaccgcaac gacgccggcg      480

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      540

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      600

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      660

gccaccacaa gaagggcgag gcccgccgca gccgccgcgc cgugacccug cccagccaca      720

gcacccgcaa gcugcagacc cgcagccaga ccuggcugga gagccgcgag uacaccaagc      780

accugauccg cguggagaac uggaucuucc gcaaccccgg cuucgcccug gccgccgccg      840

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      900

ugcugaucgc ccccgccuac agcauccgcu gcaucggcgu gagcaaccgc gacuucgugg      960

agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc ugcgugaccg     1020

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca     1080

uggccgaggu gcgcagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagcc     1140

gcugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca     1200

agcgcacccu gguggaccgc ggcuggggca acggcugcgg ccuguucggc aagggcagcc     1260

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1320

agaaccugga guaccgcauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1380

ugaacgacac cggccacgag accgacgaga accgcgccaa gguggagauc acccccaaca     1440

gcccccgcgc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagcccc     1500

gcaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1560

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1620

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagcgcc     1680

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1740

uggaggccga gauggacggc gccaagggcc gccugagcag cggccaccug aagugccgcc     1800

ugaagaugga caagcugcgc cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1860

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1920

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1980

cccccguggg ccgccugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     2040

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     2100

agaagaagau cacccaccac uggcaccgca gcggcagcac caucggcaag gccuucgagg     2160

ccaccgugcg cggcgccaag cgcauggccg ugcugggcga caccgccugg gacuucggca     2220

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     2280

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2340

uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug gcccugggcg     2400

gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcagc guggacuuca     2460

gcaagaagga gacccgcugc ggcaccggcg uguucgugua caacgacgug gaggccuggc     2520

gcgaccgcua caaguaccac cccgacagcc cccgccgccu ggccgccgcc gugaagcagg     2580

ccugggagga cggcaucugc ggcaucagca gcgugagccg cauggagaac aucauguggc     2640

gcagcgugga gggcgagcug aacgccaucc uggaggagaa cggcgugcag cugaccgugg     2700

uggugggcag cgugaagaac cccauguggc gcggccccca gcgccugccc gugcccguga     2760

acgagcugcc ccacggcugg aaggccuggg gcaagagcca cuucgugcgc gccgccaaga     2820

ccaacaacag cuucguggug gacggcgaca cccugaagga gugcccccug aagcaccgcg     2880

ccuggaacag cuuccuggug gaggaccacg gcuucggcgu guuccacacc agcguguggc     2940

ugaaggugcg cgaggacuac agccuggagu gcgaccccgc cgugaucggc accgccguga     3000

agggcaagga ggccgugcac agcgaccugg gcuacuggau cgagagcgag aagaacgaca     3060

ccuggcgccu gaagcgcgcc caccugaucg agaugaagac cugcgagugg cccaagagcc     3120

acacccugug gaccgacggc aucgaggaga gcgaccugau cauccccaag agccuggccg     3180

gcccccugag ccaccacaac acccgcgagg gcuaccgcac ccagaugaag ggccccuggc     3240

acagcgagga gcuggagauc cgcuucgagg agugccccgg caccaaggug cacguggagg     3300

agaccugcgg cacccgcggc cccagccugc gcagcaccac cgccagcggc cgcgugaucg     3360

aggaguggug cugccgcgag ugcaccaugc ccccccugag cuuccgcgcc aaggacggcu     3420

gcugguacgg cauggagauc cgcccccgca aggagcccga gagcaaccug gugcgcagca     3480

uggugaccgc cggcagcacc gaccacaugg accacuucag ccugggcgug cuggugaucc     3540

ugcugauggu gcaggagggc cugaagaagc gcaugaccac caagaucauc aucagcacca     3600

gcauggccgu gcugguggcc augauccugg gcggcuucag caugagcgac cuggccaagc     3660

uggccauccu gaugggcgcc accuucgccg agaugaacac cggcggcgac guggcccacc     3720

uggcccugau cgccgccuuc aaggugcgcc ccgcccugcu ggugagcuuc aucuuccgcg     3780

ccaacuggac cccccgcgag agcaugcugc uggcccuggc cagcugccug cugcagaccg     3840

ccaucagcgc ccuggagggc gaccugaugg ugcugaucaa cggcuucgcc cuggccuggc     3900

uggccauccg cgccauggug gugccccgca ccgacaacau cacccuggcc auccuggccg     3960

cccugacccc ccuggcccgc ggcacccugc ugguggccug gcgcgccggc cuggccaccu     4020

gcggcggcuu caugcugcug agccugaagg gcaagggcag cgugaagaag aaccugcccu     4080

ucgugauggc ccugggccug accgccgugc gccuggugga ccccaucaac guggugggcc     4140

ugcugcugcu gacccgcagc ggcaagcgca gcuggccccc cagcgaggug cugaccgccg     4200

ugggccugau cugcgcccug gccggcggcu ucgccaaggc cgacaucgag auggccggcc     4260

ccauggccgc cgugggccug cugaucguga gcuacguggu gagcggcaag agcguggaca     4320

uguacaucga gcgcgccggc gacaucaccu gggagaagga cgccgaggug accggcaaca     4380

gcccccgccu ggacguggcc cuggacgaga gcggcgacuu cagccuggug gaggacgacg     4440

gcccccccau gcgcgagauc auccugaagg uggugcugau gaccaucugc ggcaugaacc     4500

ccaucgccau ccccuucgcc gccggcgccu gguacgugua cgugaagacc ggcaagcgca     4560

gcggcgcccu gugggacgug cccgccccca aggaggugaa gaagggcgag accaccgacg     4620

gcguguaccg cgugaugacc cgccgccugc ugggcagcac ccaggugggc gugggcguga     4680

ugcaggaggg cguguuccac accauguggc acgugaccaa gggcagcgcc cugcgcagcg     4740

gcgagggccg ccuggacccc uacuggggcg acgugaagca ggaccuggug agcuacugcg     4800

gccccuggaa gcuggacgcc gccugggacg gccacagcga ggugcagcug cuggccgugc     4860

cccccggcga gcgcgcccgc aacauccaga cccugcccgg caucuucaag accaaggacg     4920

gcgacaucgg cgccguggcc cuggacuacc ccgccggcac cagcggcagc cccauccugg     4980

acaagugcgg ccgcgugauc ggccuguacg gcaacggcgu ggugaucaag aacggcagcu     5040

acgugagcgc caucacccag ggccgccgcg aggaggagac ccccguggag ugcuucgagc     5100

ccagcaugcu gaagaagaag cagcugaccg ugcuggaccu gcaccccggc gccggcaaga     5160

cccgccgcgu gcugcccgag aucgugcgcg aggccaucaa gacccgccug cgcaccguga     5220

uccuggcccc cacccgcgug guggccgccg agauggagga ggcccugcgc ggccugcccg     5280

ugcgcuacau gaccaccgcc gugaacguga cccacagcgg caccgagauc guggaccuga     5340

ugugccacgc caccuucacc agccgccugc ugcagcccau ccgcgugccc aacuacaacc     5400

uguacaucau ggacgaggcc cacuucaccg accccagcag caucgccgcc cgcggcuaca     5460

ucagcacccg cguggagaug ggcgaggccg ccgccaucuu caugaccgcc accccccccg     5520

gcacccgcga cgccuucccc gacagcaaca gccccaucau ggacaccgag guggaggugc     5580

ccgagcgcgc cuggagcagc ggcuucgacu gggugaccga cuacagcggc aagaccgugu     5640

gguucgugcc cagcgugcgc aacggcaacg agaucgccgc cugccugacc aaggccggca     5700

agcgcgugau ccagcugagc cgcaagaccu ucgagaccga guuccagaag accaagcacc     5760

aggaguggga cuucguggug accaccgaca ucagcgagau gggcgccaac uucaaggccg     5820

accgcgugau cgacagccgc cgcugccuga agcccgugau ccuggacggc gagcgcguga     5880

uccuggccgg ccccaugccc gugacccacg ccagcgccgc ccagcgccgc ggccgcaucg     5940

gccgcaaccc caacaagccc ggcgacgagu accuguacgg cggcggcugc gccgagaccg     6000

acgaggacca cgcccacugg cuggaggccc gcaugcugcu ggacaacauc uaccugcagg     6060

acggccugau cgccagccug uaccgccccg aggccgacaa gguggccgcc aucgagggcg     6120

aguucaagcu gcgcaccgag cagcgcaaga ccuucgugga gcugaugaag cgcggcgacc     6180

ugcccgugug gcuggccuac cagguggcca gcgccggcau caccuacacc gaccgccgcu     6240

ggugcuucga cggcaccacc aacaacacca ucauggagga cagcgugccc gccgaggugu     6300

ggacccgcca cggcgagaag cgcgugcuga agccccgcug gauggacgcc cgcgugugca     6360

gcgaccacgc cgcccugaag agcuucaagg aguucgccgc cggcaagcgc ggcgccgccu     6420

ucggcgugau ggaggcccug ggcacccugc ccggccacau gaccgagcgc uuccaggagg     6480

ccaucgacaa ccuggccgug cugaugcgcg ccgagaccgg cagccgcccc uacaaggccg     6540

ccgccgccca gcugcccgag acccuggaga ccaucaugcu gcugggccug cugggcaccg     6600

ugagccuggg caucuucuuc gugcugaugc gcaacaaggg caucggcaag augggcuucg     6660

gcauggugac ccugggcgcc agcgccuggc ugauguggcu gagcgagauc gagcccgccc     6720

gcaucgccug cgugcugauc gugguguucc ugcugcuggu ggugcugauc cccgagcccg     6780

agaagcagcg cagcccccag gacaaccaga uggccaucau caucauggug gccgugggcc     6840

ugcugggccu gaucaccgcc aacgagcugg gcuggcugga gcgcaccaag agcgaccuga     6900

gccaccugau gggccgccgc gaggagggcg ccaccauggg cuucagcaug gacaucgacc     6960

ugcgccccgc cagcgccugg gccaucuacg ccgcccugac caccuucauc acccccgccg     7020

ugcagcacgc cgugaccacc agcuacaaca acuacagccu gauggccaug gccacccagg     7080

ccggcgugcu guucggcaug ggcaagggca ugcccuucua cgccugggac uucggcgugc     7140

cccugcugau gaucggcugc uacagccagc ugaccccccu gacccugauc guggccauca     7200

uccugcuggu ggcccacuac auguaccuga uccccggccu gcaggccgcc gccgcccgcg     7260

ccgcccagaa gcgcaccgcc gccggcauca ugaagaaccc cgugguggac ggcaucgugg     7320

ugaccgacau cgacaccaug accaucgacc cccaggugga gaagaagaug ggccaggugc     7380

ugcugauggc cguggccgug agcagcgcca uccugagccg caccgccugg ggcuggggcg     7440

aggccggcgc ccugaucacc gccgccacca gcacccugug ggagggcagc cccaacaagu     7500

acuggaacag cagcaccgcc accagccugu gcaacaucuu ccgcggcagc uaccuggccg     7560

gcgccagccu gaucuacacc gugacccgca acgccggccu ggugaagcgc cgcggcggcg     7620

gcaccggcga gacccugggc gagaagugga aggcccgccu gaaccagaug agcgcccugg     7680

aguucuacag cuacaagaag agcggcauca ccgaggugug ccgcgaggag gcccgccgcg     7740

cccugaagga cggcguggcc accggcggcc acgccgugag ccgcggcagc gccaagcugc     7800

gcuggcuggu ggagcgcggc uaccugcagc ccuacggcaa ggugaucgac cugggcugcg     7860

gccgcggcgg cuggagcuac uacgccgcca ccauccgcaa ggugcaggag gugaagggcu     7920

acaccaaggg cggccccggc cacgaggagc ccgugcuggu gcagagcuac ggcuggaaca     7980

ucgugcgccu gaagagcggc guggacgugu uccacauggc cgccgagccc ugcgacaccc     8040

ugcugugcga caucggcgag agcagcagca gccccgaggu ggaggaggcc cgcacccugc     8100

gcgugcugag cauggugggc gacuggcugg agaagcgccc cggcgccuuc ugcaucaagg     8160

ugcugugccc cuacaccagc accaugaugg agacccugga gcgccugcag cgccgcuacg     8220

gcggcggccu ggugcgcgug ccccugagcc gcaacagcac ccacgagaug uacuggguga     8280

gcggcgccaa gagcaacacc aucaagagcg ugagcaccac cagccagcug cugcugggcc     8340

gcauggacgg cccccgccgc cccgugaagu acgaggagga cgugaaccug ggcagcggca     8400

cccgcgccgu ggugagcugc gccgaggccc ccaacaugaa gaucaucggc aaccgcaucg     8460

agcgcauccg cagcgagcac gccgagaccu gguucuucga cgagaaccac cccuaccgca     8520

ccugggccua ccacggcagc uacgaggccc ccacccaggg cagcgccagc agccugauca     8580

acggcguggu gcgccugcug agcaagcccu gggacguggu gaccggcgug accggcaucg     8640

ccaugaccga caccaccccc uacggccagc agcgcguguu caaggagaag guggacaccc     8700

gcgugcccga cccccaggag ggcacccgcc aggugaugag cauggugagc agcuggcugu     8760

ggaaggagcu gggcaagcac aagcgccccc gcgugugcac caaggaggag uucaucaaca     8820

aggugcgcag caacgccgcc cugggcgcca ucuucgagga ggagaaggag uggaagaccg     8880

ccguggaggc cgugaacgac ccccgcuucu gggcccuggu ggacaaggag cgcgagcacc     8940

accugcgcgg cgagugccag agcugcgugu acaacaugau gggcaagcgc gagaagaagc     9000

agggcgaguu cggcaaggcc aagggcagcc gcgccaucug guacaugugg cugggcgccc     9060

gcuuccugga guucgaggcc cugggcuucc ugaacgagga ccacuggaug ggccgcgaga     9120

acagcggcgg cggcguggag ggccugggcc ugcagcgccu gggcuacgug cuggaggaga     9180

ugagccgcau ccccggcggc cgcauguacg ccgacgacac cgccggcugg gacacccgca     9240

ucagccgcuu cgaccuggag aacgaggccc ugaucaccaa ccagauggag aagggccacc     9300

gcgcccuggc ccuggccauc aucaaguaca ccuaccagaa caagguggug aaggugcugc     9360

gccccgccga gaagggcaag accgugaugg acaucaucag ccgccaggac cagcgcggca     9420

gcggccaggu ggugaccuac gcccugaaca ccuucaccaa ccugguggug cagcugaucc     9480

gcaacaugga ggccgaggag gugcuggaga ugcaggaccu guggcugcug cgccgcagcg     9540

agaaggugac caacuggcug cagagcaacg gcugggaccg ccugaagcgc auggccguga     9600

gcggcgacga cugcguggug aagcccaucg acgaccgcuu cgcccacgcc cugcgcuucc     9660

ugaacgacau gggcaaggug cgcaaggaca cccaggagug gaagcccagc accggcuggg     9720

acaacuggga ggaggugccc uucugcagcc accacuucaa caagcugcac cugaaggacg     9780

gccgcagcau cguggugccc ugccgccacc aggacgagcu gaucggccgc gcccgcguga     9840

gccccggcgc cggcuggagc auccgcgaga ccgccugccu ggccaagagc uacgcccaga     9900

uguggcagcu gcuguacuuc caccgccgcg accugcgccu gauggccaac gccaucugca     9960

gcagcgugcc cguggacugg gugcccaccg gccgcaccac cuggagcauc cacggcaagg    10020

gcgaguggau gaccaccgag gacaugcugg ugguguggaa ccgcgugugg aucgaggaga    10080

acgaccacau ggaggacaag acccccguga ccaaguggac cgacaucccc uaccugggca    10140

agcgcgagga ccuguggugc ggcagccuga ucggccaccg cccccgcacc accugggccg    10200

agaacaucaa gaacaccgug aacauggugc gccgcaucau cggcgacgag gagaaguaca    10260

uggacuaccu gagcacccag gugcgcuacc ugggcgagga gggcagcacc cccggcgugc    10320

uguaaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag    10380

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac    10440

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua    10500

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc    10620

ccccccaaag gcucuuuuca gagccaccag aauu                                10654


<210>  329
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 
       opimized

<400>  329
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugacccg ccgcggcagc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc      420

gccgcgccgu gacccugccc agccacagca cccgcaagcu gcagacccgc agccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugauccgcgu ggagaacugg aucuuccgca      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      660

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacaucg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  330
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 
       opimized

<400>  330
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugacccg ccgcggcagc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc      420

gccgcgccgu gacccugccc agccacagca cccgcaagcu gcagacccgc agccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugauccgcgu ggagaacugg aucuuccgca      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      660

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacaucg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca aguaaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  331
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 
       opimized

<400>  331
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      120

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacaucg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggccgcc      960

ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg caccgcagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  332
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 
       opimized

<400>  332
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      120

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacaucg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggccgcc      960

ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg caccgcagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu aaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  333
<211>  10654
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) complete polyprotein mRNA Design 2 
       optimized

<400>  333
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

accccaagaa gaagagcggc ggcuuccgca ucgugaacau gcugaagcgc ggcguggccc      120

gcgugagccc cuucggcggc cugaagcgcc ugcccgccgg ccugcugcug ggccacggcc      180

ccauccgcau ggugcuggcc auccuggccu uccugcgcuu caccgccauc aagcccagcc      240

ugggccugau caaccgcugg ggcagcgugg gcaagaagga ggccauggag aucaucaaga      300

aguucaagaa ggaccuggcc gccaugcugc gcaucaucaa cgcccgcaag gagaagaagc      360

gccgcggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc gccauggccg      420

ccgaggugac ccgccgcggc agcgccuacu acauguaccu ggaccgcaac gacgccggcg      480

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      540

ugggccacac cugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      600

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      660

gccaccacaa gaagggcgag gcccgccgca gccgccgcgc cgugacccug cccagccaca      720

gcacccgcaa gcugcagacc cgcagccaga ccuggcugga gagccgcgag uacaccaagc      780

accugauccg cguggagaac uggaucuucc gcaaccccgg cuucgcccug gccgccgccg      840

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      900

ugcugaucgc ccccgccuac agcauccgcu gcaucggcgu gagcaaccgc gacuucgugg      960

agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc ugcgugaccg     1020

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca     1080

uggccgaggu gcgcagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagcc     1140

gcugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca     1200

agcgcacccu gguggaccgc ggcuggggca acggcugcgg ccuguucggc aagggcagcc     1260

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1320

agaaccugga guaccgcauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1380

ugaacgacac cggccacgag accgacgaga accgcgccaa gguggagauc acccccaaca     1440

gcccccgcgc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagcccc     1500

gcaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1560

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1620

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagcgcc     1680

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1740

uggaggccga gauggacggc gccaagggcc gccugagcag cggccaccug aagugccgcc     1800

ugaagaugga caagcugcgc cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1860

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1920

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1980

cccccguggg ccgccugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     2040

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     2100

agaagaagau cacccaccac uggcaccgca gcggcagcac caucggcaag gccuucgagg     2160

ccaccgugcg cggcgccaag cgcauggccg ugcugggcga caccgccugg gacuucggca     2220

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     2280

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2340

uguggcuggg ccugaacgcc aagaacggca gcaucagccu gaugugccug gcccugggcg     2400

gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcagc guggacuuca     2460

gcaagaagga gacccgcugc ggcaccggcg uguucgugua caacgacgug gaggccuggc     2520

gcgaccgcua caaguaccac cccgacagcc cccgccgccu ggccgccgcc gugaagcagg     2580

ccugggagga cggcaucugc ggcaucagca gcgugagccg cauggagaac aucauguggc     2640

gcagcgugga gggcgagcug aacgccaucc uggaggagaa cggcgugcag cugaccgugg     2700

uggugggcag cgugaagaac cccauguggc gcggccccca gcgccugccc gugcccguga     2760

acgagcugcc ccacggcugg aaggccuggg gcaagagcua cuucgugcgc gccgccaaga     2820

ccaacaacag cuucguggug gacggcgaca cccugaagga gugcccccug aagcaccgcg     2880

ccuggaacag cuuccuggug gaggaccacg gcuucggcgu guuccacacc agcguguggc     2940

ugaaggugcg cgaggacuac agccuggagu gcgaccccgc cgugaucggc accgccguga     3000

agggcaagga ggccgugcac agcgaccugg gcuacuggau cgagagcgag aagaacgaca     3060

ccuggcgccu gaagcgcgcc caccugaucg agaugaagac cugcgagugg cccaagagcc     3120

acacccugug gaccgacggc aucgaggaga gcgaccugau cauccccaag agccuggccg     3180

gcccccugag ccaccacaac acccgcgagg gcuaccgcac ccagaugaag ggccccuggc     3240

acagcgagga gcuggagauc cgcuucgagg agugccccgg caccaaggug cacguggagg     3300

agaccugcgg cacccgcggc cccagccugc gcagcaccac cgccagcggc cgcgugaucg     3360

aggaguggug cugccgcgag ugcaccaugc ccccccugag cuuccgcgcc aaggacggcu     3420

gcugguacgg cauggagauc cgcccccgca aggagcccga gagcaaccug gugcgcagca     3480

uggugaccgc cggcagcacc gaccacaugg accacuucag ccugggcgug cuggugaucc     3540

ugcugauggu gcaggagggc cugaagaagc gcaugaccac caagaucauc aucagcacca     3600

gcauggccgu gcugguggcc augauccugg gcggcuucag caugagcgac cuggccaagc     3660

uggccauccu gaugggcgcc accuucgccg agaugaacac cggcggcgac guggcccacc     3720

uggcccugau cgccgccuuc aaggugcgcc ccgcccugcu ggugagcuuc aucuuccgcg     3780

ccaacuggac cccccgcgag agcaugcugc uggcccuggc cagcugccug cugcagaccg     3840

ccaucagcgc ccuggagggc gaccugaugg ugcugaucaa cggcuucgcc cuggccuggc     3900

uggccauccg cgccauggug gugccccgca ccgacaacau cacccuggcc auccuggccg     3960

cccugacccc ccuggcccgc ggcacccugc ugguggccug gcgcgccggc cuggccaccu     4020

gcggcggcuu caugcugcug agccugaagg gcaagggcag cgugaagaag aaccugcccu     4080

ucgugauggc ccugggccug accgccgugc gccuggugga ccccaucaac guggugggcc     4140

ugcugcugcu gacccgcagc ggcaagcgca gcuggccccc cagcgaggug cugaccgccg     4200

ugggccugau cugcgcccug gccggcggcu ucgccaaggc cgacaucgag auggccggcc     4260

ccauggccgc cgugggccug cugaucguga gcuacguggu gagcggcaag agcguggaca     4320

uguacaucga gcgcgccggc gacaucaccu gggagaagga cgccgaggug accggcaaca     4380

gcccccgccu ggacguggcc cuggacgaga gcggcgacuu cagccuggug gaggacgacg     4440

gcccccccau gcgcgagauc auccugaagg uggugcugau gaccaucugc ggcaugaacc     4500

ccaucgccau ccccuucgcc gccggcgccu gguacgugua cgugaagacc ggcaagcgca     4560

gcggcgcccu gugggacgug cccgccccca aggaggugaa gaagggcgag accaccgacg     4620

gcguguaccg cgugaugacc cgccgccugc ugggcagcac ccaggugggc gugggcguga     4680

ugcaggaggg cguguuccac accauguggc acgugaccaa gggcagcgcc cugcgcagcg     4740

gcgagggccg ccuggacccc uacuggggcg acgugaagca ggaccuggug agcuacugcg     4800

gccccuggaa gcuggacgcc gccugggacg gccacagcga ggugcagcug cuggccgugc     4860

cccccggcga gcgcgcccgc aacauccaga cccugcccgg caucuucaag accaaggacg     4920

gcgacaucgg cgccguggcc cuggacuacc ccgccggcac cagcggcagc cccauccugg     4980

acaagugcgg ccgcgugauc ggccuguacg gcaacggcgu ggugaucaag aacggcagcu     5040

acgugagcgc caucacccag ggccgccgcg aggaggagac ccccguggag ugcuucgagc     5100

ccagcaugcu gaagaagaag cagcugaccg ugcuggaccu gcaccccggc gccggcaaga     5160

cccgccgcgu gcugcccgag aucgugcgcg aggccaucaa gacccgccug cgcaccguga     5220

uccuggcccc cacccgcgug guggccgccg agauggagga ggcccugcgc ggccugcccg     5280

ugcgcuacau gaccaccgcc gugaacguga cccacagcgg caccgagauc guggaccuga     5340

ugugccacgc caccuucacc agccgccugc ugcagcccau ccgcgugccc aacuacaacc     5400

uguacaucau ggacgaggcc cacuucaccg accccagcag caucgccgcc cgcggcuaca     5460

ucagcacccg cguggagaug ggcgaggccg ccgccaucuu caugaccgcc accccccccg     5520

gcacccgcga cgccuucccc gacagcaaca gccccaucau ggacaccgag guggaggugc     5580

ccgagcgcgc cuggagcagc ggcuucgacu gggugaccga ccacagcggc aagaccgugu     5640

gguucgugcc cagcgugcgc aacggcaacg agaucgccgc cugccugacc aaggccggca     5700

agcgcgugau ccagcugagc cgcaagaccu ucgagaccga guuccagaag accaagcacc     5760

aggaguggga cuucguggug accaccgaca ucagcgagau gggcgccaac uucaaggccg     5820

accgcgugau cgacagccgc cgcugccuga agcccgugau ccuggacggc gagcgcguga     5880

uccuggccgg ccccaugccc gugacccacg ccagcgccgc ccagcgccgc ggccgcaucg     5940

gccgcaaccc caacaagccc ggcgacgagu accuguacgg cggcggcugc gccgagaccg     6000

acgaggacca cgcccacugg cuggaggccc gcaugcugcu ggacaacauc uaccugcagg     6060

acggccugau cgccagccug uaccgccccg aggccgacaa gguggccgcc aucgagggcg     6120

aguucaagcu gcgcaccgag cagcgcaaga ccuucgugga gcugaugaag cgcggcgacc     6180

ugcccgugug gcuggccuac cagguggcca gcgccggcau caccuacacc gaccgccgcu     6240

ggugcuucga cggcaccacc aacaacacca ucauggagga cagcgugccc gccgaggugu     6300

ggacccgcca cggcgagaag cgcgugcuga agccccgcug gauggacgcc cgcgugugca     6360

gcgaccacgc cgcccugaag agcuucaagg aguucgccgc cggcaagcgc ggcgccgccu     6420

ucggcgugau ggaggcccug ggcacccugc ccggccacau gaccgagcgc uuccaggagg     6480

ccaucgacaa ccuggccgug cugaugcgcg ccgagaccgg cagccgcccc uacaaggccg     6540

ccgccgccca gcugcccgag acccuggaga ccaucaugcu gcugggccug cugggcaccg     6600

ugagccuggg caucuucuuc gugcugaugc gcaacaaggg caucggcaag augggcuucg     6660

gcauggugac ccugggcgcc agcgccuggc ugauguggcu gagcgagauc gagcccgccc     6720

gcaucgccug cgugcugauc gugguguucc ugcugcuggu ggugcugauc cccgagcccg     6780

agaagcagcg cagcccccag gacaaccaga uggccaucau caucauggug gccgugggcc     6840

ugcugggccu gaucaccgcc aacgagcugg gcuggcugga gcgcaccaag agcgaccuga     6900

gccaccugau gggccgccgc gaggagggcg ccaccaucgg cuucagcaug gacaucgacc     6960

ugcgccccgc cagcgccugg gccaucuacg ccgcccugac caccuucauc acccccgccg     7020

ugcagcacgc cgugaccacc agcuacaaca acuacagccu gauggccaug gccacccagg     7080

ccggcgugcu guucggcaug ggcaagggca ugcccuucua cgccugggac uucggcgugc     7140

cccugcugau gaucggcugc uacagccagc ugaccccccu gacccugauc guggccauca     7200

uccugcuggu ggcccacuac auguaccuga uccccggccu gcaggccgcc gccgcccgcg     7260

ccgcccagaa gcgcaccgcc gccggcauca ugaagaaccc cgugguggac ggcaucgugg     7320

ugaccgacau cgacaccaug accaucgacc cccaggugga gaagaagaug ggccaggugc     7380

ugcugaucgc cguggccgug agcagcgcca uccugagccg caccgccugg ggcuggggcg     7440

aggccggcgc ccugaucacc gccgccacca gcacccugug ggagggcagc cccaacaagu     7500

acuggaacag cagcaccgcc accagccugu gcaacaucuu ccgcggcagc uaccuggccg     7560

gcgccagccu gaucuacacc gugacccgca acgccggccu ggugaagcgc cgcggcggcg     7620

gcaccggcga gacccugggc gagaagugga aggcccgccu gaaccagaug agcgcccugg     7680

aguucuacag cuacaagaag agcggcauca ccgaggugug ccgcgaggag gcccgccgcg     7740

cccugaagga cggcguggcc accggcggcc acgccgugag ccgcggcagc gccaagcugc     7800

gcuggcuggu ggagcgcggc uaccugcagc ccuacggcaa ggugaucgac cugggcugcg     7860

gccgcggcgg cuggagcuac uacgccgcca ccauccgcaa ggugcaggag gugaagggcu     7920

acaccaaggg cggccccggc cacgaggagc ccgugcuggu gcagagcuac ggcuggaaca     7980

ucgugcgccu gaagagcggc guggacgugu uccacauggc cgccgagccc ugcgacaccc     8040

ugcugugcga caucggcgag agcagcagca gccccgaggu ggaggaggcc cgcacccugc     8100

gcgugcugag cauggugggc gacuggcugg agaagcgccc cggcgccuuc ugcaucaagg     8160

ugcugugccc cuacaccagc accaugaugg agacccugga gcgccugcag cgccgcuacg     8220

gcggcggccu ggugcgcgug ccccugagcc gcaacagcac ccacgagaug uacuggguga     8280

gcggcgccaa gagcaacacc aucaagagcg ugagcaccac cagccagcug cugcugggcc     8340

gcauggacgg cccccgccgc cccgugaagu acgaggagga cgugaaccug ggcagcggca     8400

cccgcgccgu ggugagcugc gccgaggccc ccaacaugaa gaucaucggc aaccgcaucg     8460

agcgcauccg cagcgagcac gccgagaccu gguucuucga cgagaaccac cccuaccgca     8520

ccugggccua ccacggcagc uacgaggccc ccacccaggg cagcgccagc agccugauca     8580

acggcguggu gcgccugcug agcaagcccu gggacguggu gaccggcgug accggcaucg     8640

ccaugaccga caccaccccc uacggccagc agcgcguguu caaggagaag guggacaccc     8700

gcgugcccga cccccaggag ggcacccgcc aggugaugag cauggugagc agcuggcugu     8760

ggaaggagcu gggcaagcac aagcgccccc gcgugugcac caaggaggag uucaucaaca     8820

aggugcgcag caacgccgcc cugggcgcca ucuucgagga ggagaaggag uggaagaccg     8880

ccguggaggc cgugaacgac ccccgcuucu gggcccuggu ggacaaggag cgcgagcacc     8940

accugcgcgg cgagugccag agcugcgugu acaacaugau gggcaagcgc gagaagaagc     9000

agggcgaguu cggcaaggcc aagggcagcc gcgccaucug guacaugugg cugggcgccc     9060

gcuuccugga guucgaggcc cugggcuucc ugaacgagga ccacuggaug ggccgcgaga     9120

acagcggcgg cggcguggag ggccugggcc ugcagcgccu gggcuacgug cuggaggaga     9180

ugagccgcau ccccggcggc cgcauguacg ccgacgacac cgccggcugg gacacccgca     9240

ucagccgcuu cgaccuggag aacgaggccc ugaucaccaa ccagauggag aagggccacc     9300

gcgcccuggc ccuggccauc aucaaguaca ccuaccagaa caagguggug aaggugcugc     9360

gccccgccga gaagggcaag accgugaugg acaucaucag ccgccaggac cagcgcggca     9420

gcggccaggu ggugaccuac gcccugaaca ccuucaccaa ccugguggug cagcugaucc     9480

gcaacaugga ggccgaggag gugcuggaga ugcaggaccu guggcugcug cgccgcagcg     9540

agaaggugac caacuggcug cagagcaacg gcugggaccg ccugaagcgc auggccguga     9600

gcggcgacga cugcguggug aagcccaucg acgaccgcuu cgcccacgcc cugcgcuucc     9660

ugaacgacau gggcaaggug cgcaaggaca cccaggagug gaagcccagc accggcuggg     9720

acaacuggga ggaggugccc uucugcagcc accacuucaa caagcugcac cugaaggacg     9780

gccgcagcau cguggugccc ugccgccacc aggacgagcu gaucggccgc gcccgcguga     9840

gccccggcgc cggcuggagc auccgcgaga ccgccugccu ggccaagagc uacgcccaga     9900

uguggcagcu gcuguacuuc caccgccgcg accugcgccu gauggccaac gccaucugca     9960

gcagcgugcc cguggacugg gugcccaccg gccgcaccac cuggagcauc cacggcaagg    10020

gcgaguggau gaccaccgag gacaugcugg ugguguggaa ccgcgugugg aucgaggaga    10080

acgaccacau ggaggacaag acccccguga ccaaguggac cgacaucccc uaccugggca    10140

agcgcgagga ccuguggugc ggcagccuga ucggccaccg cccccgcacc accugggccg    10200

agaacaucaa gaacaccgug aacauggugc gccgcaucau cggcgacgag gagaaguaca    10260

uggacuaccu gagcacccag gugcgcuacc ugggcgagga gggcagcacc cccggcgugc    10320

uguaaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag    10380

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac    10440

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua    10500

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc    10620

ccccccaaag gcucuuuuca gagccaccag aauu                                10654


<210>  334
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 
       opimized

<400>  334
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugacccg ccgcggcagc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaccug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc      420

gccgcgccgu gacccugccc agccacagca cccgcaagcu gcagacccgc agccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugauccgcgu ggagaacugg aucuuccgca      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      660

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  335
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 
       opimized

<400>  335
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugacccg ccgcggcagc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaccug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc      420

gccgcgccgu gacccugccc agccacagca cccgcaagcu gcagacccgc agccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugauccgcgu ggagaacugg aucuuccgca      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      660

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca aguaaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  336
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 
       opimized

<400>  336
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      120

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggccgcc      960

ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg caccgcagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  337
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-719) mRNA Design 2 
       opimized

<400>  337
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      120

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu ggagaucacc cccaacagcc cccgcgccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagcgccaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggccgcc      960

ugagcagcgg ccaccugaag ugccgccuga agauggacaa gcugcgccug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggccg ccugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg caccgcagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu aaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  338
<211>  10642
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) complete polyprotein mRNA Design 2 optimized

<400>  338
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

accccaagga ggagauccgc cgcauccgca ucgugaacau gcugaagcgc ggcguggccc      120

gcgugaaccc ccugggcggc cugaagcgcc ugcccgccgg ccugcugcug ggccacggcc      180

ccauccgcau ggugcuggcc auccuggccu uccugcgcuu caccgccauc aagcccagcc      240

ugggccugau caaccgcugg ggcagcgugg gcaagaagga ggccauggag aucaucaaga      300

aguucaagaa ggaccuggcc gccaugcugc gcaucaucaa cgcccgcaag gagcgcaagc      360

gccgcggcgc cgacaccagc aucggcauca ucggccugcu gcugaccacc gccauggccg      420

ccgagaucac ccgccgcggc agcgccuacu acauguaccu ggaccgcagc gacgccggca      480

aggccaucag cuucgccacc acccugggcg ugaacaagug ccacgugcag aucauggacc      540

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      600

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      660

gccaccacaa gaagggcgag gcccgccgca gccgccgcgc cgugacccug cccagccaca      720

gcacccgcaa gcugcagacc cgcagccaga ccuggcugga gagccgcgag uacaccaagc      780

accugaucaa gguggagaac uggaucuucc gcaaccccgg cuucgcccug guggccgugg      840

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      900

ugcugaucgc ccccgccuac agcauccgcu gcaucggcgu gagcaaccgc gacuucgugg      960

agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc ugcgugaccg     1020

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca     1080

uggccgaggu gcgcagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagcc     1140

gcugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca     1200

agcgcacccu gguggaccgc ggcuggggca acggcugcgg ccuguucggc aagggcagcc     1260

uggugaccug cgccaaguuc accugcagca agaagaugac cggcaagagc auccagcccg     1320

agaaccugga guaccgcauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1380

gcuacgagac cgacgaggac cgcgccaagg uggaggugac ccccaacagc ccccgcgccg     1440

aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagccccgc accggccugg     1500

acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug cacaaggagu     1560

gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc ccccacugga     1620

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagcgccag accguggugg     1680

ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug gaggccgaga     1740

uggacggcgc caagggccgc cuguucagcg gccaccugaa gugccgccug aagauggaca     1800

agcugcgccu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc uucaccaagg     1860

ugcccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg     1920

gccccugcaa gauccccgug cagauggccg uggacaugca gacccugacc cccgugggcc     1980

gccugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag augaugcugg     2040

agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgac aagaagauca     2100

cccaccacug gcaccgcagc ggcagcacca ucggcaaggc cuucgaggcc accgugcgcg     2160

gcgccaagcg cauggccgug cugggcgaca ccgccuggga cuucggcagc gugggcggcg     2220

uguucaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc aagagccugu     2280

ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcuggug uggcugggcc     2340

ugaacaccaa gaacggcagc aucagccuga ccugccuggc ccugggcggc gugaugaucu     2400

uccugagcac cgccgugagc gccgacgugg gcugcagcgu ggacuucagc aagaaggaga     2460

cccgcugcgg caccggcgug uucaucuaca acgacgugga ggccuggcgc gaccgcuaca     2520

aguaccaccc cgacagcccc cgccgccugg ccgccgccgu gaagcaggcc ugggaggagg     2580

gcaucugcgg caucagcagc gugagccgca uggagaacau cauguggaag agcguggagg     2640

gcgagcugaa cgccauccug gaggagaacg gcgugcagcu gaccguggug gugggcagcg     2700

ugaagaaccc cauguggcgc ggcccccagc gccugcccgu gcccgugaac gagcugcccc     2760

acggcuggaa ggccuggggc aagagcuacu ucgugcgcgc cgccaagacc aacaacagcu     2820

ucguggugga cggcgacacc cugaaggagu gcccccugga gcaccgcgcc uggaacagcu     2880

uccuggugga ggaccacggc uucggcgugu uccacaccag cguguggcug aaggugcgcg     2940

aggacuacag ccuggagugc gaccccgccg ugaucggcac cgccgugaag ggccgcgagg     3000

ccgcccacag cgaccugggc uacuggaucg agagcgagaa gaacgacacc uggcgccuga     3060

agcgcgccca ccugaucgag augaagaccu gcgaguggcc caagagccac acccugugga     3120

ccgacggcgu ggaggagagc gaccugauca uccccaagag ccuggccggc ccccugagcc     3180

accacaacac ccgcgagggc uaccgcaccc aggugaaggg ccccuggcac agcgaggagc     3240

uggagauccg cuucgaggag ugccccggca ccaaggugua cguggaggag accugcggca     3300

cccgcggccc cagccugcgc agcaccaccg ccagcggccg cgugaucgag gaguggugcu     3360

gccgcgagug caccaugccc ccccugagcu uccgcgccaa ggacggcugc ugguacggca     3420

uggagauccg cccccgcaag gagcccgaga gcaaccuggu gcgcagcaug gugaccgccg     3480

gcagcaccga ccacauggac cacuucagcc ugggcgugcu ggugauccug cugauggugc     3540

aggagggccu gaagaagcgc augaccacca agaucaucau gagcaccagc auggccgugc     3600

ugguggugau gauccugggc ggcuucagca ugagcgaccu ggccaagcug gugauccuga     3660

ugggcgccac cuucgccgag augaacaccg gcggcgacgu ggcccaccug gcccuggugg     3720

ccgccuucaa ggugcgcccc gcccugcugg ugagcuucau cuuccgcgcc aacuggaccc     3780

cccgcgagag caugcugcug gcccuggcca gcugccugcu gcagaccgcc aucagcgccc     3840

uggagggcga ccugauggug cugaucaacg gcuucgcccu ggccuggcug gccauccgcg     3900

ccauggccgu gccccgcacc gacaacaucg cccugcccau ccuggccgcc cugacccccc     3960

uggcccgcgg cacccugcug guggccuggc gcgccggccu ggccaccugc ggcggcauca     4020

ugcugcugag ccugaagggc aagggcagcg ugaagaagaa ccugcccuuc gugauggccc     4080

ugggccugac cgccgugcgc gugguggacc ccaucaacgu ggugggccug cugcugcuga     4140

cccgcagcgg caagcgcagc uggcccccca gcgaggugcu gaccgccgug ggccugaucu     4200

gcgcccuggc cggcggcuuc gccaaggccg acaucgagau ggccggcccc auggccgccg     4260

ugggccugcu gaucgugagc uacgugguga gcggcaagag cguggacaug uacaucgagc     4320

gcgccggcga caucaccugg gagaaggacg ccgaggugac cggcaacagc ccccgccugg     4380

acguggcccu ggacgagagc ggcgacuuca gccuggugga ggaggacggc ccccccaugc     4440

gcgagaucau ccugaaggug gugcugaugg ccaucugcgg caugaacccc aucgccaucc     4500

ccuucgccgc cggcgccugg uacguguacg ugaagaccgg caagcgcagc ggcgcccugu     4560

gggacgugcc cgcccccaag gaggugaaga agggcgagac caccgacggc guguaccgcg     4620

ugaugacccg ccgccugcug ggcagcaccc aggugggcgu gggcgugaug caggagggcg     4680

uguuccacac cauguggcac gugaccaagg gcgccgcccu gcgcagcggc gagggccgcc     4740

uggaccccua cuggggcgac gugaagcagg accuggugag cuacugcggc cccuggaagc     4800

uggacgccgc cugggacggc cugagcgagg ugcagcugcu ggccgugccc cccggcgagc     4860

gcgcccgcaa cauccagacc cugcccggca ucuucaagac caaggacggc gacaucggcg     4920

ccguggcccu ggacuacccc gccggcacca gcggcagccc cauccuggac aagugcggcc     4980

gcgugaucgg ccuguacggc aacggcgugg ugaucaagaa cggcagcuac gugagcgcca     5040

ucacccaggg caagcgcgag gaggagaccc ccguggagug cuucgagccc agcaugcuga     5100

agaagaagca gcugaccgug cuggaccugc accccggcgc cggcaagacc cgccgcgugc     5160

ugcccgagau cgugcgcgag gccaucaaga agcgccugcg caccgugauc cuggccccca     5220

cccgcguggu ggccgccgag auggaggagg cccugcgcgg ccugcccgug cgcuacauga     5280

ccaccgccgu gaacgugacc cacagcggca ccgagaucgu ggaccugaug ugccacgcca     5340

ccuucaccag ccgccugcug cagcccaucc gcgugcccaa cuacaaccug aacaucaugg     5400

acgaggccca cuucaccgac cccagcagca ucgccgcccg cggcuacauc agcacccgcg     5460

uggagauggg cgaggccgcc gccaucuuca ugaccgccac cccccccggc acccgcgacg     5520

ccuuccccga cagcaacagc cccaucaugg acaccgaggu ggaggugccc gagcgcgccu     5580

ggagcagcgg cuucgacugg gugaccgacc acagcggcaa gaccgugugg uucgugccca     5640

gcgugcgcaa cggcaacgag aucgccgccu gccugaccaa ggccggcaag cgcgugaucc     5700

agcugagccg caagaccuuc gagaccgagu uccagaagac caagaaccag gagugggacu     5760

ucgugaucac caccgacauc agcgagaugg gcgccaacuu caaggccgac cgcgugaucg     5820

acagccgccg cugccugaag cccgugaucc uggacggcga gcgcgugauc cuggccggcc     5880

ccaugcccgu gacccacgcc agcgccgccc agcgccgcgg ccgcaucggc cgcaacccca     5940

acaagcccgg cgacgaguac auguacggcg gcggcugcgc cgagaccgac gagggccacg     6000

cccacuggcu ggaggcccgc augcugcugg acaacaucua ccugcaggac ggccugaucg     6060

ccagccugua ccgccccgag gccgacaagg uggccgccau cgagggcgag uucaagcugc     6120

gcaccgagca gcgcaagacc uucguggagc ugaugaagcg cggcgaccug cccguguggc     6180

uggccuacca gguggccagc gccggcauca ccuacaccga ccgccgcugg ugcuucgacg     6240

gcaccaccaa caacaccauc auggaggaca gcgugcccgc cgaggugugg accaaguacg     6300

gcgagaagcg cgugcugaag ccccgcugga uggacgcccg cgugugcagc gaccacgccg     6360

cccugaagag cuucaaggag uucgccgccg gcaagcgcgg cgccgcccug ggcgugaugg     6420

aggcccuggg cacccugccc ggccacauga ccgagcgcuu ccaggaggcc aucgacaacc     6480

uggccgugcu gaugcgcgcc gagaccggca gccgccccua caaggccgcc gccgcccagc     6540

ugcccgagac ccuggagacc aucaugcugc ugggccugcu gggcaccgug agccugggca     6600

ucuucuucgu gcugaugcgc aacaagggca ucggcaagau gggcuucggc auggugaccc     6660

ugggcgccag cgccuggcug auguggcuga gcgagaucga gcccgcccgc aucgccugcg     6720

ugcugaucgu gguguuccug cugcuggugg ugcugauccc cgagcccgag aagcagcgca     6780

gcccccagga caaccagaug gccaucauca ucaugguggc cgugggccug cugggccuga     6840

ucaccgccaa cgagcugggc uggcuggagc gcaccaagaa cgacaucgcc caccugaugg     6900

gccgccgcga ggagggcgcc accaugggcu ucagcaugga caucgaccug cgccccgcca     6960

gcgccugggc caucuacgcc gcccugacca cccugaucac ccccgccgug cagcacgccg     7020

ugaccaccag cuacaacaac uacagccuga uggccauggc cacccaggcc ggcgugcugu     7080

ucggcauggg caagggcaug cccuucaugc acggcgaccu gggcgugccc cugcugauga     7140

ugggcugcua cagccagcug accccccuga cccugaucgu ggccaucauc cugcuggugg     7200

cccacuacau guaccugauc cccggccugc aggccgccgc cgcccgcgcc gcccagaagc     7260

gcaccgccgc cggcaucaug aagaaccccg ugguggacgg caucguggug accgacaucg     7320

acaccaugac caucgacccc cagguggaga agaagauggg ccaggugcug cugaucgccg     7380

uggccaucag cagcgccgug cugcugcgca ccgccugggg cuggggcgag gccggcgccc     7440

ugaucaccgc cgccaccagc acccuguggg agggcagccc caacaaguac uggaacagca     7500

gcaccgccac cagccugugc aacaucuucc gcggcagcua ccuggccggc gccagccuga     7560

ucuacaccgu gacccgcaac gccggccugg ugaagcgccg cggcggcggc accggcgaga     7620

cccugggcga gaaguggaag gcccgccuga accagaugag cgcccuggag uucuacagcu     7680

acaagaagag cggcaucacc gaggugugcc gcgaggaggc ccgccgcgcc cugaaggacg     7740

gcguggccac cggcggccac gccgugagcc gcggcagcgc caagauccgc uggcuggagg     7800

agcgcggcua ccugcagccc uacggcaagg ugguggaccu gggcugcggc cgcggcggcu     7860

ggagcuacua cgccgccacc auccgcaagg ugcaggaggu gcgcggcuac accaagggcg     7920

gccccggcca cgaggagccc augcuggugc agagcuacgg cuggaacauc gugcgccuga     7980

agagcggcgu ggacguguuc cacauggccg ccgagcccug cgacacccug cugugcgaca     8040

ucggcgagag cagcagcagc cccgaggugg aggagacccg cacccugcgc gugcugagca     8100

uggugggcga cuggcuggag aagcgccccg gcgccuucug caucaaggug cugugccccu     8160

acaccagcac caugauggag accauggagc gccugcagcg ccgccacggc ggcggccugg     8220

ugcgcgugcc ccugugccgc aacagcaccc acgagaugua cugggugagc ggcgccaaga     8280

gcaacaucau caagagcgug agcaccacca gccagcugcu gcugggccgc auggacggcc     8340

cccgccgccc cgugaaguac gaggaggacg ugaaccuggg cagcggcacc cgcgccgugg     8400

ccagcugcgc cgaggccccc aacaugaaga ucaucggccg ccgcaucgag cgcauccgca     8460

acgagcacgc cgagaccugg uuccuggacg agaaccaccc cuaccgcacc ugggccuacc     8520

acggcagcua cgaggccccc acccagggca gcgccagcag ccuggugaac ggcguggugc     8580

gccugcugag caagcccugg gacgugguga ccggcgugac cggcaucgcc augaccgaca     8640

ccacccccua cggccagcag cgcguguuca aggagaaggu ggacacccgc gugcccgacc     8700

cccaggaggg cacccgccag gugaugaaca ucgugagcag cuggcugugg aaggagcugg     8760

gcaagcgcaa gcgcccccgc gugugcacca aggaggaguu caucaacaag gugcgcagca     8820

acgccgcccu gggcgccauc uucgaggagg agaaggagug gaagaccgcc guggaggccg     8880

ugaacgaccc ccgcuucugg gcccuggugg accgcgagcg cgagcaccac cugcgcggcg     8940

agugccacag cugcguguac aacaugaugg gcaagcgcga gaagaagcag ggcgaguucg     9000

gcaaggccaa gggcagccgc gccaucuggu acauguggcu gggcgcccgc uuccuggagu     9060

ucgaggcccu gggcuuccug aacgaggacc acuggauggg ccgcgagaac agcggcggcg     9120

gcguggaggg ccugggccug cagcgccugg gcuacauccu ggaggagaug aaccgcgccc     9180

ccggcggcaa gauguacgcc gacgacaccg ccggcuggga cacccgcauc agcaaguucg     9240

accuggagaa cgaggcccug aucaccaacc agauggagga gggccaccgc acccuggccc     9300

uggccgugau caaguacacc uaccagaaca agguggugaa ggugcugcgc cccgccgagg     9360

gcggcaagac cgugauggac aucaucagcc gccaggacca gcgcggcagc ggccaggugg     9420

ugaccuacgc ccugaacacc uucaccaacc ugguggugca gcugauccgc aacauggagg     9480

ccgaggaggu gcuggagaug caggaccugu ggcugcugcg caagcccgag aaggugaccc     9540

gcuggcugca gagcaacggc ugggaccgcc ugaagcgcau ggccgugagc ggcgacgacu     9600

gcguggugaa gcccaucgac gaccgcuucg cccacgcccu gcgcuuccug aacgacaugg     9660

gcaaggugcg caaggacacc caggagugga agcccagcac cggcuggagc aacugggagg     9720

aggugcccuu cugcagccac cacuucaaca agcuguaccu gaaggacggc cgcagcaucg     9780

uggugcccug ccgccaccag gacgagcuga ucggccgcgc ccgcgugagc cccggcgccg     9840

gcuggagcau ccgcgagacc gccugccugg ccaagagcua cgcccagaug uggcagcugc     9900

uguacuucca ccgccgcgac cugcgccuga uggccaacgc caucugcagc gccgugcccg     9960

uggacugggu gcccaccggc cgcaccaccu ggagcaucca cggcaagggc gaguggauga    10020

ccaccgagga caugcugaug guguggaacc gcguguggau cgaggagaac gaccacaugg    10080

aggacaagac ccccgugacc aaguggaccg acauccccua ccugggcaag cgcgaggacc    10140

uguggugcgg cagccugauc ggccaccgcc cccgcaccac cugggccgag aacaucaagg    10200

acaccgugaa cauggugcgc cgcaucaucg gcgacgagga gaaguacaug gacuaccuga    10260

gcacccaggu gcgcuaccug ggcgaggagg gcagcacccc cggcgugcug uaaggacuag    10320

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc    10380

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa    10440

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga    10500

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    10560

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc    10620

ucuuuucaga gccaccagaa uu                                             10642


<210>  339
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opimized

<400>  339
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucacccg ccgcggcagc gccuacuaca      180

uguaccugga ccgcagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc      420

gccgcgccgu gacccugccc agccacagca cccgcaagcu gcagacccgc agccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugaucaaggu ggagaacugg aucuuccgca      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      660

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggaccgc gccaaggugg     1140

aggugacccc caacagcccc cgcgccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gccccgcacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gcgccagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggccgccug uucagcggcc     1500

accugaagug ccgccugaag auggacaagc ugcgccugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1680

acaugcagac ccugaccccc gugggccgcc ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgacaag aagaucaccc accacuggca ccgcagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgugu ucaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcuggugugg cugggccuga acaccaagaa cggcagcauc agccugaccu     2100

gccuggcccu gggcggcgug augaucuucc ugagcaccgc cgugagcgcc gacgugggcu     2160

gcagcgugga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  340
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opimized

<400>  340
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucacccg ccgcggcagc gccuacuaca      180

uguaccugga ccgcagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc      420

gccgcgccgu gacccugccc agccacagca cccgcaagcu gcagacccgc agccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugaucaaggu ggagaacugg aucuuccgca      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      660

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggaccgc gccaaggugg     1140

aggugacccc caacagcccc cgcgccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gccccgcacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gcgccagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggccgccug uucagcggcc     1500

accugaagug ccgccugaag auggacaagc ugcgccugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1680

acaugcagac ccugaccccc gugggccgcc ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgacaag aagaucaccc accacuggca ccgcagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgugu ucaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcuggugugg cugggccuga acaccaagaa cggcagcauc agccugaccu     2100

gccuggcccu gggcggcgug augaucuucc ugagcaccgc cgugagcgcc gacgugggcu     2160

gcagcgugga cuucagcaag uaaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  341
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opimized

<400>  341
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      120

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggaccgc gccaaggugg      600

aggugacccc caacagcccc cgcgccgagg ccacccuggg cggcuucggc agccugggcc      660

uggacugcga gccccgcacc ggccuggacu ucagcgaccu guacuaccug accaugaaca      720

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg      780

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg      840

cccacgccaa gcgccagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg      900

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggccgccug uucagcggcc      960

accugaagug ccgccugaag auggacaagc ugcgccugaa gggcgugagc uacagccugu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1080

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1140

acaugcagac ccugaccccc gugggccgcc ugaucaccgc caaccccgug aucaccgaga     1200

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1260

ugaucggcgu gggcgacaag aagaucaccc accacuggca ccgcagcggc agcaccaucg     1320

gcaaggccuu cgaggccacc gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  342
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opimized

<400>  342
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc auccgcugca      120

ucggcgugag caaccgcgac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugcg cagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccgcggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggaccgc gccaaggugg      600

aggugacccc caacagcccc cgcgccgagg ccacccuggg cggcuucggc agccugggcc      660

uggacugcga gccccgcacc ggccuggacu ucagcgaccu guacuaccug accaugaaca      720

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg      780

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg      840

cccacgccaa gcgccagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg      900

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggccgccug uucagcggcc      960

accugaagug ccgccugaag auggacaagc ugcgccugaa gggcgugagc uacagccugu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1080

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1140

acaugcagac ccugaccccc gugggccgcc ugaucaccgc caaccccgug aucaccgaga     1200

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1260

ugaucggcgu gggcgacaag aagaucaccc accacuggca ccgcagcggc agcaccaucg     1320

gcaaggccuu cgaggccacc gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg     1380

ccugggacuu cggcagcuaa ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  343
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  343

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala 
        


<210>  344
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  344

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala 
        


<210>  345
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  345

Gly Ala Asp Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala 
        


<210>  346
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  346
ggcgcagaua cuagugucgg aauuguuggc cuccugcuga ccacagcuau ggca             54


<210>  347
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  347
ggcgcagaua cuagugucgg aauuguuggc cuccugcuga ccacagcuau ggca             54


<210>  348
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  348
ggcgcagaca ccagcaucgg aaucauuggc cuccugcuga cuacagccau ggca             54


<210>  349
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  349
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcg             54


<210>  350
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  350
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcg             54


<210>  351
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  351
ggcgccgaca ccagcaucgg gaucaucggc cugcugcuca cgaccgccau ggcg             54


<210>  352
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  352
ggcgccgaua ccagcguggg cauugugggc cugcugcuga caacagccau ggcc             54


<210>  353
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  353
ggcgccgaua ccagcguggg cauugugggc cugcugcuga caacagccau ggcc             54


<210>  354
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  354
ggcgccgaua ccagcaucgg caucaucggc cugcugcuga ccacagccau ggcc             54


<210>  355
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  355
ggcgccgaca ccagcguggg caucgugggc cugcugcuga caaccgccau ggcc             54


<210>  356
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  356
ggcgccgaca ccagcguggg caucgugggc cugcugcuga caaccgccau ggcc             54


<210>  357
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  357
ggcgccgaca ccagcaucgg caucaucggc cugcugcuga ccaccgccau ggcc             54


<210>  358
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  358
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggcc             54


<210>  359
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  359
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggcc             54


<210>  360
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  360
ggcgccgaca ccagcaucgg caucaucggc cugcugcuga ccaccgccau ggcc             54


<210>  361
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) X

<400>  361

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg 
1               5                   10          


<210>  362
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) X

<400>  362

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg 
1               5                   10          


<210>  363
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) X

<400>  363

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg 
1               5                   10          


<210>  364
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) X

<400>  364
agaauaauca augcuaggaa ggagaagaag agacgg                                 36


<210>  365
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) X

<400>  365
agaauaauca augcuaggaa ggagaagaag agacga                                 36


<210>  366
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) X

<400>  366
agaauaauca augcuaggaa agagaggaag agacgu                                 36


<210>  367
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) X

<400>  367
cggaucauca acgcccgcaa ggagaagaag cggcgc                                 36


<210>  368
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) X

<400>  368
cggaucauca acgcccgcaa ggagaagaag cggcgc                                 36


<210>  369
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) X

<400>  369
cggaucauca acgcccgcaa ggagcggaag cgccgg                                 36


<210>  370
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) X

<400>  370
cggaucauca acgcccggaa agagaagaag cgcaga                                 36


<210>  371
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) X

<400>  371
cggaucauca acgcccggaa agagaagaag cgcaga                                 36


<210>  372
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) X

<400>  372
cggaucauca acgcccggaa agagcggaag cggaga                                 36


<210>  373
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) X

<400>  373
cggaucauca acgcccggaa agagaagaag cgcaga                                 36


<210>  374
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) X

<400>  374
cggaucauca acgcccggaa agagaagaag cgcaga                                 36


<210>  375
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) X

<400>  375
cggaucauca acgcccggaa agagcggaag cggaga                                 36


<210>  376
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) X

<400>  376
cgcaucauca acgcccgcaa ggagaagaag cgccgc                                 36


<210>  377
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) X

<400>  377
cgcaucauca acgcccgcaa ggagaagaag cgccgc                                 36


<210>  378
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) X

<400>  378
cgcaucauca acgcccgcaa ggagcgcaag cgccgc                                 36


<210>  379
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) XX

<400>  379

Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
1               5                   10  


<210>  380
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) XX

<400>  380

Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
1               5                   10  


<210>  381
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) XX

<400>  381

Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
1               5                   10  


<210>  382
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) XX

<400>  382
gauguggggu gcucggugga cuucucaaag                                        30


<210>  383
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) XX

<400>  383
gauguggggu gcucggugga cuucucaaag                                        30


<210>  384
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) XX

<400>  384
gacguggggu gcucagugga cuucucaaaa                                        30


<210>  385
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) XX

<400>  385
gacguggggu gcuccgucga cuucagcaag                                        30


<210>  386
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) XX

<400>  386
gacgucgggu gcuccgugga cuucagcaag                                        30


<210>  387
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) XX

<400>  387
gacguggggu gcuccgugga cuucagcaag                                        30


<210>  388
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) XX

<400>  388
gacgugggcu gcagcgugga cuucagcaaa                                        30


<210>  389
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) XX

<400>  389
gacgugggcu gcagcgugga cuucagcaaa                                        30


<210>  390
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) XX

<400>  390
gaugugggcu guagcgugga cuucagcaaa                                        30


<210>  391
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) XX

<400>  391
gacgugggcu gcagcgugga cuucuccaaa                                        30


<210>  392
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) XX

<400>  392
gacgugggcu gcagcgugga cuucuccaaa                                        30


<210>  393
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) XX

<400>  393
gacgugggcu gcagcgugga cuucuccaaa                                        30


<210>  394
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) XX

<400>  394
gacgugggcu gcagcgugga cuucagcaag                                        30


<210>  395
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) XX

<400>  395
gacgugggcu gcagcgugga cuucagcaag                                        30


<210>  396
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) XX

<400>  396
gacgugggcu gcagcgugga cuucagcaag                                        30


<210>  397
<211>  794
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-prME

<400>  397

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
    130                 135                 140                 


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
        435                 440                 445             


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
    450                 455                 460                 


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
465                 470                 475                 480 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
                485                 490                 495     


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
            500                 505                 510         


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
        515                 520                 525             


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
    530                 535                 540                 


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
545                 550                 555                 560 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
            580                 585                 590         


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
        595                 600                 605             


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
    610                 615                 620                 


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
625                 630                 635                 640 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
                645                 650                 655     


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
        675                 680                 685             


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
    690                 695                 700                 


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
                725                 730                 735     


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
        755                 760                 765             


Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
    770                 775                 780                 


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
785                 790                 


<210>  398
<211>  794
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-prME

<400>  398

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
    130                 135                 140                 


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Thr Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
        435                 440                 445             


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
    450                 455                 460                 


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
465                 470                 475                 480 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
                485                 490                 495     


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
            500                 505                 510         


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
        515                 520                 525             


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
    530                 535                 540                 


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
545                 550                 555                 560 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
            580                 585                 590         


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
        595                 600                 605             


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
    610                 615                 620                 


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
625                 630                 635                 640 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
                645                 650                 655     


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
        675                 680                 685             


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
    690                 695                 700                 


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
                725                 730                 735     


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
        755                 760                 765             


Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
    770                 775                 780                 


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
785                 790                 


<210>  399
<211>  790
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-prME

<400>  399

Met Lys Asn Pro Lys Glu Glu Ile Arg Arg Ile Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Asn Pro Leu Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr Ser Ile Gly Ile 
            100                 105                 110         


Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Ile Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp Ala Gly Lys Ala 
    130                 135                 140                 


Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys His Val Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu 
        435                 440                 445             


Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala 
    450                 455                 460                 


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
465                 470                 475                 480 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
                485                 490                 495     


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
            500                 505                 510         


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
        515                 520                 525             


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
    530                 535                 540                 


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
545                 550                 555                 560 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys 
                565                 570                 575     


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
            580                 585                 590         


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu 
        595                 600                 605             


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
    610                 615                 620                 


Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
625                 630                 635                 640 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
                645                 650                 655     


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
            660                 665                 670         


Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg 
        675                 680                 685             


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
    690                 695                 700                 


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
705                 710                 715                 720 


Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
                725                 730                 735     


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
            740                 745                 750         


Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly 
        755                 760                 765             


Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu 
    770                 775                 780                 


Ser Thr Ala Val Ser Ala 
785                 790 


<210>  400
<211>  2382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-prME

<400>  400
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcaauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac ggggcgcaga uacuaguguc ggaauuguug gccuccugcu gaccacagcu      360

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      420

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      480

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      540

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      600

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      660

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      720

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      780

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      900

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      960

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc     1020

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg     1080

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa     1200

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc     1260

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1320

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1380

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1440

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1500

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1560

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1620

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1680

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1740

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1800

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1860

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1920

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1980

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     2040

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     2100

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     2160

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     2220

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     2280

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2340

uuagggggag uguugaucuu cuuauccaca gccgucucug cu                        2382


<210>  401
<211>  2382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-prME

<400>  401
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcgauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac gaggcgcaga uacuaguguc ggaauuguug gccuccugcu gaccacagcu      360

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      420

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      480

auggaucuug gacacacgug ugaugccacc augagcuaug aaugcccuau gcuggaugag      540

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      600

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      660

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      720

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      780

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      900

uuuguggaag guaugucagg ugggacuugg guugauguug ucuuggaaca uggagguugu      960

gucacuguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc     1020

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg     1080

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa     1200

gggagccugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc     1260

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1320

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaagu ugagauaacg     1380

cccaauucac caagagccga agccacccug gggggguuug gaagccuagg acuugauugu     1440

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1500

uggcugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1560

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1620

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1680

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1740

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguacugca     1800

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1860

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1920

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1980

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     2040

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     2100

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     2160

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aaucuuugga     2220

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     2280

uugcugaugu gguugggucu gaacgcaaag aauggaucua uuucccuuau gugcuuggcc     2340

uuagggggag uguugaucuu cuuauccaca gccgucucug cu                        2382


<210>  402
<211>  2370
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-prME

<400>  402
augaaaaacc ccaaagaaga aauccggagg auccggauug ucaauaugcu aaaacgcgga       60

guagcccgug uaaaccccuu gggagguuug aagagguugc cagccggacu ucugcugggu      120

cauggaccca ucagaauggu uuuggcgaua cuagccuuuu ugagauuuac agcaaucaag      180

ccaucacugg gccuuaucaa cagauggggu uccgugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuugcugcc auguugagaa uaaucaaugc uaggaaagag      300

aggaagagac guggcgcaga caccagcauc ggaaucauug gccuccugcu gacuacagcc      360

auggcagcag agaucacuag acgcgggagu gcauacuaca uguacuugga uaggagcgau      420

gccgggaagg ccauuucguu ugcuaccaca uugggaguga acaagugcca cguacagauc      480

auggaccucg ggcacaugug ugacgccacc augaguuaug agugcccuau gcuggaugag      540

ggaguggaac cagaugaugu cgauugcugg ugcaacacga caucaacuug gguuguguac      600

ggaaccuguc aucacaaaaa aggugaggca cggcgaucua gaagagccgu gacgcucccu      660

ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu gguuagaauc aagagaauac      720

acgaagcacu ugaucaaggu ugaaaacugg auauucagga accccggguu ugcgcuagug      780

gccguugcca uugccuggcu uuugggaagc ucgacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagu aucaggugca uuggagucag caauagagac      900

uucguggagg gcaugucagg ugggaccugg guugauguug ucuuggaaca uggaggcugc      960

guuaccguga uggcacagga caagccaaca gucgacauag aguuggucac gacgacgguu     1020

aguaacaugg ccgagguaag auccuauugc uacgaggcau cgauaucgga cauggcuucg     1080

gacagucguu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacauuagu ggacagaggu uggggaaacg guuguggacu uuuuggcaaa     1200

gggagcuugg ugacaugugc caaguuuacg uguucuaaga agaugaccgg gaagagcauu     1260

caaccggaaa aucuggagua ucggauaaug cuaucagugc auggcuccca gcauagcggg     1320

augauuggau augaaacuga cgaagauaga gcgaaagucg agguuacgcc uaauucacca     1380

agagcggaag caaccuuggg aggcuuugga agcuuaggac uugacuguga accaaggaca     1440

ggccuugacu uuucagaucu guauuaccug accaugaaca auaagcauug guuggugcac     1500

aaagaguggu uucaugacau cccauugccu uggcaugcug gggcagacac cggaacucca     1560

cacuggaaca acaaagaggc auugguagaa uucaaggaug cccacgccaa gaggcaaacc     1620

gucgucguuc uggggagcca ggaaggagcc guucacacgg cucucgcugg agcucuagag     1680

gcugagaugg auggugcaaa gggaaggcug uucucuggcc auuugaaaug ccgccuaaaa     1740

auggacaagc uuagauugaa gggcguguca uauuccuugu gcacugcggc auucacauuc     1800

accaaggucc cagcugaaac acugcaugga acagucacag uggaggugca guaugcaggg     1860

acagauggac ccugcaagau cccaguccag auggcggugg acaugcagac ccugacccca     1920

guuggaaggc ugauaaccgc caaccccgug auuacugaaa gcacugagaa cucaaagaug     1980

auguuggagc uugacccacc auuuggggau ucuuacauug ucauaggagu uggggacaag     2040

aaaaucaccc accacuggca uaggaguggu agcaccaucg gaaaggcauu ugaggccacu     2100

gugagaggcg ccaagagaau ggcaguccug ggggauacag ccugggacuu cggaucaguc     2160

gggggugugu ucaacucacu ggguaagggc auucaccaga uuuuuggagc agccuucaaa     2220

ucacuguuug gaggaauguc cugguucuca cagauccuca uaggcacgcu gcuagugugg     2280

uuagguuuga acacaaagaa uggaucuauc ucccucacau gcuuggcccu ggggggagug     2340

augaucuucc ucuccacggc uguuucugcu                                      2370


<210>  403
<211>  2382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-prME

<400>  403
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc      360

auggcggccg aggucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      420

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      480

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      600

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      660

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      720

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      780

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      840

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      900

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      960

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug     1020

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc     1080

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac     1140

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc     1260

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1320

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1380

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1440

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1560

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1620

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1680

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1740

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1860

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1920

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1980

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     2040

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     2100

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     2160

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     2220

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     2280

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2340

cugggcggcg uccugaucuu ccucuccacc gcggugagcg cc                        2382


<210>  404
<211>  2382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-prME

<400>  404
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc      360

auggcggccg aggucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      420

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      480

auggaccugg ggcacacgug cgacgcgacc auguccuacg agugccccau gcucgacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca cgagcaccug ggucguguac      600

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      660

agccacucca cgcgcaagcu gcagacccgg agccagaccu ggcucgaguc ccgcgaguac      720

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      780

gcggccgcca ucgccuggcu ccuggggagc uccacgagcc agaaggugau cuaccuggug      840

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      900

uucguggagg ggauguccgg cggcaccugg guggacgucg ugcuggagca cgggggcugc      960

gugaccguca uggcccagga caagcccacc guggacaucg agcucgugac gaccaccguc     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggccu ccaucagcga cauggccucc     1080

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacgcaguac     1140

gucugcaagc gcacccuggu ggaccggggc ugggggaacg gcugcggccu cuucgggaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc     1260

cagccggaga accuggagua ccgcaucaug cucuccgucc acggcagcca gcacuccggg     1320

augaucguga acgacacggg ccacgagacc gacgagaacc gggccaaggu ggagaucacc     1380

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1440

gagccccgga cgggccugga cuucagcgac cuguacuacc ucaccaugaa caacaagcac     1500

uggcuggucc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1560

accgggaccc cgcacuggaa caacaaggag gcccucgugg aguucaagga cgcgcacgcc     1620

aagcgccaga cgguggucgu gcugggcucc caggaggggg ccgugcacac cgcccuggcc     1680

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1740

ugccgccuga agauggacaa gcuccggcug aaggggguca gcuacucccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgcggag acgcuccacg gcaccgugac cguggagguc     1860

caguacgccg ggaccgacgg cccgugcaag gugcccgccc agauggccgu ggacaugcag     1920

acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg ugaucaccga gagcacggag     1980

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     2040

gucggggaga agaagaucac ccaccacugg caccggagcg gcuccaccau cgggaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc ugggggacac ggcgugggac     2160

uucggcagcg ucggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     2220

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacc     2280

cugcugaugu ggcucgggcu gaacgccaag aacggcucca ucagccugau gugccucgcg     2340

cugggcggcg ugcugaucuu ccucuccacc gccgugagcg cc                        2382


<210>  405
<211>  2370
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-prME

<400>  405
augaagaacc ccaaggagga gauccgccgg auccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg ugaacccgcu cgggggccug aagcggcugc ccgccgggcu ccugcugggc      120

cacgggccca uccgcauggu gcucgcgauc cuggccuucc ugcgguucac cgccaucaag      180

cccagccucg gccugaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcuccgga ucaucaacgc ccgcaaggag      300

cggaagcgcc ggggcgccga caccagcauc gggaucaucg gccugcugcu cacgaccgcc      360

auggcggccg agaucacccg ccgggggucc gccuacuaca uguaccugga ccgcagcgac      420

gccggcaagg ccaucuccuu cgcgaccacg cugggcguga acaagugcca cgugcagauc      480

auggaccucg ggcacaugug cgacgccacc augagcuacg agugcccgau gcuggacgag      540

ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca ccuccacgug gguggucuac      600

gggaccugcc accacaagaa gggcgaggcc cggcgcagcc ggcgcgccgu gacccugccc      660

ucccacagca cccggaagcu ccagacgcgc ucccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugaucaaggu ggagaacugg aucuuccgga accccggcuu cgcccucguc      780

gcgguggcca ucgccuggcu gcuggggucc agcaccuccc agaaggugau cuaccucguc      840

augauccugc ugaucgcccc ggccuacagc auccgcugca ucggcguguc caaccgggac      900

uucguggagg ggaugagcgg cgggacgugg gucgacgugg ugcucgagca cggcggcugc      960

gucaccguga uggcgcagga caagcccacc guggacaucg agcuggucac cacgaccgug     1020

uccaacaugg ccgagguccg cagcuacugc uacgaggcca gcaucuccga cauggccagc     1080

gacucccggu gccccaccca gggggaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacgcucgu ggaccggggc ugggggaacg gcugcgggcu guucggcaag     1200

ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga agaugaccgg gaaguccauc     1260

cagcccgaga accucgagua ccgcaucaug cugagcgugc acggcuccca gcacagcggg     1320

augaucggcu acgagacgga cgaggaccgg gccaaggugg aggucacccc gaacuccccg     1380

cgcgccgagg ccacccuggg cggguucggc agccucgggc uggacugcga gccccggacc     1440

ggccuggacu ucuccgaccu cuacuaccug acgaugaaca acaagcacug gcuggugcac     1500

aaggaguggu uccacgacau cccccucccg uggcacgcgg gggccgacac cggcaccccg     1560

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1620

gucguggugc ugggcagcca ggagggggcg guccacacgg cccuggccgg cgcccucgag     1680

gccgagaugg acggggcgaa gggccggcug uucuccgggc accugaagug ccgccucaag     1740

auggacaagc ugcggcugaa gggcgugagc uacucccucu gcaccgccgc cuucaccuuc     1800

accaaggugc ccgccgagac ccugcacggc acggucaccg uggaggugca guacgccggg     1860

accgacggcc ccugcaagau cccgguccag auggcggugg acaugcagac ccugacgccc     1920

guggggcgcc ucaucaccgc caaccccguc aucaccgaga gcaccgagaa cuccaagaug     1980

augcuggagc uggacccgcc guucggcgac agcuacaucg ugaucggggu gggcgacaag     2040

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     2100

guccgcggcg cgaagcggau ggccgugcuc ggggacaccg ccugggacuu cggcagcgug     2160

ggcggcgucu ucaacucccu ggggaagggg auccaccaga ucuucggcgc cgccuucaag     2220

agccuguucg ggggcauguc cugguucagc cagauccuca ucgggacgcu gcuggugugg     2280

cucggccuga acaccaagaa cggcuccauc agccugaccu gccucgcgcu gggcggcgug     2340

augaucuucc uguccaccgc cgucagcgcc                                      2370


<210>  406
<211>  2382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-prME

<400>  406
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gcggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc      360

auggccgccg aggugacccg ccgcggcagc gccuacuaca uguaccugga ccgcaacgac      420

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      480

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugauccgcgu ggagaacugg aucuuccgca accccggcuu cgcccuggcc      780

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucguga acgacaccgg ccacgagacc gacgagaacc gcgccaaggu ggagaucacc     1380

cccaacagcc cccgcgccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1440

gagccccgca ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1560

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1620

aagcgccaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1680

ggcgcccugg aggccgagau ggacggcgcc aagggccgcc ugagcagcgg ccaccugaag     1740

ugccgccuga agauggacaa gcugcgccug aagggcguga gcuacagccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1860

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1920

acccugaccc ccgugggccg ccugaucacc gccaaccccg ugaucaccga gagcaccgag     1980

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     2040

gugggcgaga agaagaucac ccaccacugg caccgcagcg gcagcaccau cggcaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc ugggcgacac cgccugggac     2160

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     2220

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     2280

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2340

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg cc                        2382


<210>  407
<211>  2382
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-prME

<400>  407
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gcggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc      360

auggccgccg aggugacccg ccgcggcagc gccuacuaca uguaccugga ccgcaacgac      420

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      480

auggaccugg gccacaccug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugauccgcgu ggagaacugg aucuuccgca accccggcuu cgcccuggcc      780

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucguga acgacaccgg ccacgagacc gacgagaacc gcgccaaggu ggagaucacc     1380

cccaacagcc cccgcgccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1440

gagccccgca ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1560

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1620

aagcgccaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1680

ggcgcccugg aggccgagau ggacggcgcc aagggccgcc ugagcagcgg ccaccugaag     1740

ugccgccuga agauggacaa gcugcgccug aagggcguga gcuacagccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1860

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1920

acccugaccc ccgugggccg ccugaucacc gccaaccccg ugaucaccga gagcaccgag     1980

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     2040

gugggcgaga agaagaucac ccaccacugg caccgcagcg gcagcaccau cggcaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc ugggcgacac cgccugggac     2160

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     2220

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     2280

cugcugaugu ggcugggccu gaacgccaag aacggcagca ucagccugau gugccuggcc     2340

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg cc                        2382


<210>  408
<211>  2370
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-prME

<400>  408
augaagaacc ccaaggagga gauccgccgc auccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugaacccccu gggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

cgcaagcgcc gcggcgccga caccagcauc ggcaucaucg gccugcugcu gaccaccgcc      360

auggccgccg agaucacccg ccgcggcagc gccuacuaca uguaccugga ccgcagcgac      420

gccggcaagg ccaucagcuu cgccaccacc cugggcguga acaagugcca cgugcagauc      480

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugaucaaggu ggagaacugg aucuuccgca accccggcuu cgcccuggug      780

gccguggcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucacc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucggcu acgagaccga cgaggaccgc gccaaggugg aggugacccc caacagcccc     1380

cgcgccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gccccgcacc     1440

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1500

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1560

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1620

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1680

gccgagaugg acggcgccaa gggccgccug uucagcggcc accugaagug ccgccugaag     1740

auggacaagc ugcgccugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1800

accaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1860

accgacggcc ccugcaagau ccccgugcag auggccgugg acaugcagac ccugaccccc     1920

gugggccgcc ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1980

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     2040

aagaucaccc accacuggca ccgcagcggc agcaccaucg gcaaggccuu cgaggccacc     2100

gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     2160

ggcggcgugu ucaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     2220

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcuggugugg     2280

cugggccuga acaccaagaa cggcagcauc agccugaccu gccuggcccu gggcggcgug     2340

augaucuucc ugagcaccgc cgugagcgcc                                      2370


<210>  409
<211>  1146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-prME-NS1

<400>  409

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
    130                 135                 140                 


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
        435                 440                 445             


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
    450                 455                 460                 


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
465                 470                 475                 480 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
                485                 490                 495     


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
            500                 505                 510         


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
        515                 520                 525             


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
    530                 535                 540                 


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
545                 550                 555                 560 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
            580                 585                 590         


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
        595                 600                 605             


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
    610                 615                 620                 


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
625                 630                 635                 640 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
                645                 650                 655     


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
        675                 680                 685             


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
    690                 695                 700                 


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
                725                 730                 735     


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
        755                 760                 765             


Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
    770                 775                 780                 


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
785                 790                 795                 800 


Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr 
                805                 810                 815     


Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser 
            820                 825                 830         


Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile 
        835                 840                 845             


Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser 
    850                 855                 860                 


Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu 
865                 870                 875                 880 


Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln 
                885                 890                 895     


Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp 
            900                 905                 910         


Gly Lys Ser His Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val 
        915                 920                 925             


Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp 
    930                 935                 940                 


Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser 
945                 950                 955                 960 


Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala 
                965                 970                 975     


Val Ile Gly Thr Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu 
            980                 985                 990         


Gly Tyr Trp Ile Glu Ser Glu Lys  Asn Asp Thr Trp Arg  Leu Lys Arg 
        995                 1000                 1005             


Ala His  Leu Ile Glu Met Lys  Thr Cys Glu Trp Pro  Lys Ser His 
    1010                 1015                 1020             


Thr Leu  Trp Thr Asp Gly Ile  Glu Glu Ser Asp Leu  Ile Ile Pro 
    1025                 1030                 1035             


Lys Ser  Leu Ala Gly Pro Leu  Ser His His Asn Thr  Arg Glu Gly 
    1040                 1045                 1050             


Tyr Arg  Thr Gln Met Lys Gly  Pro Trp His Ser Glu  Glu Leu Glu 
    1055                 1060                 1065             


Ile Arg  Phe Glu Glu Cys Pro  Gly Thr Lys Val His  Val Glu Glu 
    1070                 1075                 1080             


Thr Cys  Gly Thr Arg Gly Pro  Ser Leu Arg Ser Thr  Thr Ala Ser 
    1085                 1090                 1095             


Gly Arg  Val Ile Glu Glu Trp  Cys Cys Arg Glu Cys  Thr Met Pro 
    1100                 1105                 1110             


Pro Leu  Ser Phe Arg Ala Lys  Asp Gly Cys Trp Tyr  Gly Met Glu 
    1115                 1120                 1125             


Ile Arg  Pro Arg Lys Glu Pro  Glu Ser Asn Leu Val  Arg Ser Met 
    1130                 1135                 1140             


Val Thr  Ala 
    1145     


<210>  410
<211>  1146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-prME-NS1

<400>  410

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
    130                 135                 140                 


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Thr Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
        435                 440                 445             


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
    450                 455                 460                 


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
465                 470                 475                 480 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
                485                 490                 495     


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
            500                 505                 510         


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
        515                 520                 525             


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
    530                 535                 540                 


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
545                 550                 555                 560 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
            580                 585                 590         


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
        595                 600                 605             


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
    610                 615                 620                 


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
625                 630                 635                 640 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
                645                 650                 655     


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
        675                 680                 685             


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
    690                 695                 700                 


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
                725                 730                 735     


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
        755                 760                 765             


Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
    770                 775                 780                 


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
785                 790                 795                 800 


Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr 
                805                 810                 815     


Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser 
            820                 825                 830         


Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile 
        835                 840                 845             


Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser 
    850                 855                 860                 


Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu 
865                 870                 875                 880 


Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln 
                885                 890                 895     


Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp 
            900                 905                 910         


Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val 
        915                 920                 925             


Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp 
    930                 935                 940                 


Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser 
945                 950                 955                 960 


Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala 
                965                 970                 975     


Val Ile Gly Thr Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu 
            980                 985                 990         


Gly Tyr Trp Ile Glu Ser Glu Lys  Asn Asp Thr Trp Arg  Leu Lys Arg 
        995                 1000                 1005             


Ala His  Leu Ile Glu Met Lys  Thr Cys Glu Trp Pro  Lys Ser His 
    1010                 1015                 1020             


Thr Leu  Trp Thr Asp Gly Ile  Glu Glu Ser Asp Leu  Ile Ile Pro 
    1025                 1030                 1035             


Lys Ser  Leu Ala Gly Pro Leu  Ser His His Asn Thr  Arg Glu Gly 
    1040                 1045                 1050             


Tyr Arg  Thr Gln Met Lys Gly  Pro Trp His Ser Glu  Glu Leu Glu 
    1055                 1060                 1065             


Ile Arg  Phe Glu Glu Cys Pro  Gly Thr Lys Val His  Val Glu Glu 
    1070                 1075                 1080             


Thr Cys  Gly Thr Arg Gly Pro  Ser Leu Arg Ser Thr  Thr Ala Ser 
    1085                 1090                 1095             


Gly Arg  Val Ile Glu Glu Trp  Cys Cys Arg Glu Cys  Thr Met Pro 
    1100                 1105                 1110             


Pro Leu  Ser Phe Arg Ala Lys  Asp Gly Cys Trp Tyr  Gly Met Glu 
    1115                 1120                 1125             


Ile Arg  Pro Arg Lys Glu Pro  Glu Ser Asn Leu Val  Arg Ser Met 
    1130                 1135                 1140             


Val Thr  Ala 
    1145     


<210>  411
<211>  1142
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-prME-NS1

<400>  411

Met Lys Asn Pro Lys Glu Glu Ile Arg Arg Ile Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Asn Pro Leu Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr Ser Ile Gly Ile 
            100                 105                 110         


Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Ile Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp Ala Gly Lys Ala 
    130                 135                 140                 


Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys His Val Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu 
        435                 440                 445             


Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala 
    450                 455                 460                 


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
465                 470                 475                 480 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
                485                 490                 495     


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
            500                 505                 510         


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
        515                 520                 525             


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
    530                 535                 540                 


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
545                 550                 555                 560 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys 
                565                 570                 575     


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
            580                 585                 590         


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu 
        595                 600                 605             


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
    610                 615                 620                 


Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
625                 630                 635                 640 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
                645                 650                 655     


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
            660                 665                 670         


Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg 
        675                 680                 685             


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
    690                 695                 700                 


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
705                 710                 715                 720 


Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
                725                 730                 735     


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
            740                 745                 750         


Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly 
        755                 760                 765             


Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu 
    770                 775                 780                 


Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
785                 790                 795                 800 


Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Ile Tyr Asn Asp Val Glu 
                805                 810                 815     


Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu 
            820                 825                 830         


Ala Ala Ala Val Lys Gln Ala Trp Glu Glu Gly Ile Cys Gly Ile Ser 
        835                 840                 845             


Ser Val Ser Arg Met Glu Asn Ile Met Trp Lys Ser Val Glu Gly Glu 
    850                 855                 860                 


Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val 
865                 870                 875                 880 


Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val 
                885                 890                 895     


Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser Tyr 
            900                 905                 910         


Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp 
        915                 920                 925             


Thr Leu Lys Glu Cys Pro Leu Glu His Arg Ala Trp Asn Ser Phe Leu 
    930                 935                 940                 


Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys 
945                 950                 955                 960 


Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr 
                965                 970                 975     


Ala Val Lys Gly Arg Glu Ala Ala His Ser Asp Leu Gly Tyr Trp Ile 
            980                 985                 990         


Glu Ser Glu Lys Asn Asp Thr Trp  Arg Leu Lys Arg Ala  His Leu Ile 
        995                 1000                 1005             


Glu Met  Lys Thr Cys Glu Trp  Pro Lys Ser His Thr  Leu Trp Thr 
    1010                 1015                 1020             


Asp Gly  Val Glu Glu Ser Asp  Leu Ile Ile Pro Lys  Ser Leu Ala 
    1025                 1030                 1035             


Gly Pro  Leu Ser His His Asn  Thr Arg Glu Gly Tyr  Arg Thr Gln 
    1040                 1045                 1050             


Val Lys  Gly Pro Trp His Ser  Glu Glu Leu Glu Ile  Arg Phe Glu 
    1055                 1060                 1065             


Glu Cys  Pro Gly Thr Lys Val  Tyr Val Glu Glu Thr  Cys Gly Thr 
    1070                 1075                 1080             


Arg Gly  Pro Ser Leu Arg Ser  Thr Thr Ala Ser Gly  Arg Val Ile 
    1085                 1090                 1095             


Glu Glu  Trp Cys Cys Arg Glu  Cys Thr Met Pro Pro  Leu Ser Phe 
    1100                 1105                 1110             


Arg Ala  Lys Asp Gly Cys Trp  Tyr Gly Met Glu Ile  Arg Pro Arg 
    1115                 1120                 1125             


Lys Glu  Pro Glu Ser Asn Leu  Val Arg Ser Met Val  Thr Ala 
    1130                 1135                 1140         


<210>  412
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-prME-NS1

<400>  412
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcaauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac ggggcgcaga uacuaguguc ggaauuguug gccuccugcu gaccacagcu      360

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      420

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      480

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      540

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      600

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      660

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      720

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      780

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      900

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      960

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc     1020

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg     1080

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa     1200

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc     1260

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1320

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1380

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1440

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1500

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1560

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1620

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1680

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1740

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1800

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1860

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1920

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1980

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     2040

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     2100

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     2160

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     2220

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     2280

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2340

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     2400

gacuucucaa agaaggagac gagaugcggu acaggggugu ucgucuauaa cgacguugaa     2460

gccuggaggg acagguacaa guaccauccu gacucccccc guagauuggc agcagcaguc     2520

aagcaagccu gggaagaugg uaucugcggg aucuccucug uuucaagaau ggaaaacauc     2580

auguggagau caguagaagg ggagcucaac gcaauccugg aagagaaugg aguucaacug     2640

acggucguug ugggaucugu aaaaaacccc auguggagag guccacagag auugcccgug     2700

ccugugaacg agcugcccca cggcuggaag gcuuggggga aaucgcacuu cgucagagca     2760

gcaaagacaa auaacagcuu ugucguggau ggugacacac ugaaggaaug cccacucaaa     2820

cauagagcau ggaacagcuu ucuuguggag gaucaugggu ucgggguauu ucacacuagu     2880

gucuggcuca agguuagaga agauuauuca uuagagugug auccagccgu uauuggaaca     2940

gcuguuaagg gaaaggaggc uguacacagu gaucuaggcu acuggauuga gagugagaag     3000

aaugacacau ggaggcugaa gagggcccau cugaucgaga ugaaaacaug ugaauggcca     3060

aagucccaca cauuguggac agauggaaua gaagagagug aucugaucau acccaagucu     3120

uuagcugggc cacucagcca ucacaauacc agagagggcu acaggaccca aaugaaaggg     3180

ccauggcaca gugaagagcu ugaaauucgg uuugaggaau gcccaggcac uaagguccac     3240

guggaggaaa cauguggaac aagaggacca ucucugagau caaccacugc aagcggaagg     3300

gugaucgagg aauggugcug cagggagugc acaaugcccc cacugucguu ccgggcuaaa     3360

gauggcuguu gguauggaau ggagauaagg cccaggaaag aaccagaaag caacuuagua     3420

aggucaaugg ugacugca                                                   3438


<210>  413
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-prME-NS1

<400>  413
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcgauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcugagaa uaaucaaugc uaggaaggag      300

aagaagagac gaggcgcaga uacuaguguc ggaauuguug gccuccugcu gaccacagcu      360

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      420

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      480

auggaucuug gacacacgug ugaugccacc augagcuaug aaugcccuau gcuggaugag      540

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      600

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      660

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      720

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      780

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      900

uuuguggaag guaugucagg ugggacuugg guugauguug ucuuggaaca uggagguugu      960

gucacuguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc     1020

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg     1080

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa     1200

gggagccugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc     1260

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1320

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaagu ugagauaacg     1380

cccaauucac caagagccga agccacccug gggggguuug gaagccuagg acuugauugu     1440

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1500

uggcugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1560

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1620

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1680

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1740

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguacugca     1800

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1860

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1920

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1980

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     2040

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     2100

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     2160

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aaucuuugga     2220

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     2280

uugcugaugu gguugggucu gaacgcaaag aauggaucua uuucccuuau gugcuuggcc     2340

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     2400

gacuucucaa agaaggagac gagaugcggu acaggggugu ucgucuauaa cgacguugaa     2460

gccuggaggg acagguacaa guaccauccu gacucccccc guagauuggc agcagcaguc     2520

aagcaagccu gggaagaugg uaucugcggg aucuccucug uuucaagaau ggaaaacauc     2580

auguggagau caguagaagg ggagcucaac gcaauccugg aagagaaugg aguucaacug     2640

acggucguug ugggaucugu aaaaaacccc auguggagag guccacagag auugcccgug     2700

ccugugaacg agcugcccca cggcuggaag gcuuggggga aaucguacuu cgucagagca     2760

gcaaagacaa auaacagcuu ugucguggau ggugacacac ugaaggaaug cccacucaaa     2820

cauagagcau ggaacagcuu ucuuguggag gaucaugggu ucgggguauu ucacacuagu     2880

gucuggcuca agguuagaga agauuauuca uuagagugug auccagccgu uauuggaaca     2940

gcuguuaagg gaaaggaggc uguacacagu gaucuaggcu acuggauuga gagugagaag     3000

aaugacacau ggaggcugaa gagggcccau cugaucgaga ugaaaacaug ugaauggcca     3060

aagucccaca cauuguggac agauggaaua gaagagagug aucugaucau acccaagucu     3120

uuagcugggc cacucagcca ucacaauacc agagagggcu acaggaccca aaugaaaggg     3180

ccauggcaca gugaagagcu ugaaauucgg uuugaggaau gcccaggcac uaagguccac     3240

guggaggaaa cauguggaac gagaggacca ucucugagau caaccacugc aagcggaagg     3300

gugaucgagg aauggugcug cagggagugc acaaugcccc cacugucguu ccgggcuaaa     3360

gauggcuguu gguauggaau ggagauaagg cccaggaaag aaccagaaag caacuuagua     3420

aggucaaugg ugacugca                                                   3438


<210>  414
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-prME-NS1

<400>  414
augaaaaacc ccaaagaaga aauccggagg auccggauug ucaauaugcu aaaacgcgga       60

guagcccgug uaaaccccuu gggagguuug aagagguugc cagccggacu ucugcugggu      120

cauggaccca ucagaauggu uuuggcgaua cuagccuuuu ugagauuuac agcaaucaag      180

ccaucacugg gccuuaucaa cagauggggu uccgugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuugcugcc auguugagaa uaaucaaugc uaggaaagag      300

aggaagagac guggcgcaga caccagcauc ggaaucauug gccuccugcu gacuacagcc      360

auggcagcag agaucacuag acgcgggagu gcauacuaca uguacuugga uaggagcgau      420

gccgggaagg ccauuucguu ugcuaccaca uugggaguga acaagugcca cguacagauc      480

auggaccucg ggcacaugug ugacgccacc augaguuaug agugcccuau gcuggaugag      540

ggaguggaac cagaugaugu cgauugcugg ugcaacacga caucaacuug gguuguguac      600

ggaaccuguc aucacaaaaa aggugaggca cggcgaucua gaagagccgu gacgcucccu      660

ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu gguuagaauc aagagaauac      720

acgaagcacu ugaucaaggu ugaaaacugg auauucagga accccggguu ugcgcuagug      780

gccguugcca uugccuggcu uuugggaagc ucgacgagcc aaaaagucau auacuugguc      840

augauacugc ugauugcccc ggcauacagu aucaggugca uuggagucag caauagagac      900

uucguggagg gcaugucagg ugggaccugg guugauguug ucuuggaaca uggaggcugc      960

guuaccguga uggcacagga caagccaaca gucgacauag aguuggucac gacgacgguu     1020

aguaacaugg ccgagguaag auccuauugc uacgaggcau cgauaucgga cauggcuucg     1080

gacagucguu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau     1140

gucugcaaaa gaacauuagu ggacagaggu uggggaaacg guuguggacu uuuuggcaaa     1200

gggagcuugg ugacaugugc caaguuuacg uguucuaaga agaugaccgg gaagagcauu     1260

caaccggaaa aucuggagua ucggauaaug cuaucagugc auggcuccca gcauagcggg     1320

augauuggau augaaacuga cgaagauaga gcgaaagucg agguuacgcc uaauucacca     1380

agagcggaag caaccuuggg aggcuuugga agcuuaggac uugacuguga accaaggaca     1440

ggccuugacu uuucagaucu guauuaccug accaugaaca auaagcauug guuggugcac     1500

aaagaguggu uucaugacau cccauugccu uggcaugcug gggcagacac cggaacucca     1560

cacuggaaca acaaagaggc auugguagaa uucaaggaug cccacgccaa gaggcaaacc     1620

gucgucguuc uggggagcca ggaaggagcc guucacacgg cucucgcugg agcucuagag     1680

gcugagaugg auggugcaaa gggaaggcug uucucuggcc auuugaaaug ccgccuaaaa     1740

auggacaagc uuagauugaa gggcguguca uauuccuugu gcacugcggc auucacauuc     1800

accaaggucc cagcugaaac acugcaugga acagucacag uggaggugca guaugcaggg     1860

acagauggac ccugcaagau cccaguccag auggcggugg acaugcagac ccugacccca     1920

guuggaaggc ugauaaccgc caaccccgug auuacugaaa gcacugagaa cucaaagaug     1980

auguuggagc uugacccacc auuuggggau ucuuacauug ucauaggagu uggggacaag     2040

aaaaucaccc accacuggca uaggaguggu agcaccaucg gaaaggcauu ugaggccacu     2100

gugagaggcg ccaagagaau ggcaguccug ggggauacag ccugggacuu cggaucaguc     2160

gggggugugu ucaacucacu ggguaagggc auucaccaga uuuuuggagc agccuucaaa     2220

ucacuguuug gaggaauguc cugguucuca cagauccuca uaggcacgcu gcuagugugg     2280

uuagguuuga acacaaagaa uggaucuauc ucccucacau gcuuggcccu ggggggagug     2340

augaucuucc ucuccacggc uguuucugcu gacguggggu gcucagugga cuucucaaaa     2400

aaggaaacga gauguggcac ggggguauuc aucuauaaug auguugaagc cuggagggac     2460

cgguacaagu accauccuga cuccccccgc agauuggcag cagcagucaa gcaggccugg     2520

gaagagggga ucugugggau cucauccguu ucaagaaugg aaaacaucau guggaaauca     2580

guagaagggg agcucaaugc uauccuagag gagaauggag uucaacugac aguuguugug     2640

ggaucuguaa aaaaccccau guggagaggu ccacaaagau ugccagugcc ugugaaugag     2700

cugccccaug gcuggaaagc cugggggaaa ucguauuuug uuagggcggc aaagaccaac     2760

aacaguuuug uugucgacgg ugacacacug aaggaauguc cgcuugagca cagagcaugg     2820

aauaguuuuc uuguggagga ucacggguuu ggagucuucc acaccagugu cuggcuuaag     2880

gucagagaag auuacucauu agaaugugac ccagccguca uaggaacagc uguuaaggga     2940

agggaggccg cgcacaguga ucugggcuau uggauugaaa gugaaaagaa ugacacaugg     3000

aggcugaaga gggcccaccu gauugagaug aaaacaugug aauggccaaa gucucacaca     3060

uuguggacag auggaguaga agaaagugau cuuaucauac ccaagucuuu agcuggucca     3120

cucagccacc acaacaccag agaggguuac agaacccaag ugaaagggcc auggcacagu     3180

gaagagcuug aaauccgguu ugaggaaugu ccaggcacca agguuuacgu ggaggagaca     3240

ugcggaacua gaggaccauc ucugagauca acuacugcaa guggaagggu cauugaggaa     3300

uggugcugua gggaaugcac aaugccccca cuaucguuuc gagcaaaaga cggcugcugg     3360

uauggaaugg agauaaggcc caggaaagaa ccagagagca acuuagugag gucaauggug     3420

acagcg                                                                3426


<210>  415
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-prME-NS1

<400>  415
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc      360

auggcggccg aggucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      420

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      480

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      600

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      660

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      720

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      780

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      840

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      900

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      960

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug     1020

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc     1080

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac     1140

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc     1260

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1320

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1380

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1440

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1560

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1620

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1680

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1740

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1860

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1920

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1980

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     2040

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     2100

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     2160

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     2220

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     2280

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2340

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcuccguc     2400

gacuucagca agaaggagac ccgcugcggg acgggcgugu ucguguacaa cgacgucgag     2460

gccuggcggg accgcuacaa guaccacccc gacuccccgc gccggcuggc cgccgcggug     2520

aagcaggccu gggaggacgg gaucugcggc aucagcuccg ugagccgcau ggagaacauc     2580

auguggcgga gcgucgaggg ggagcugaac gccauccucg aggagaacgg cgugcagcug     2640

accguggucg ugggcuccgu gaagaacccg auguggcgcg ggccccagcg gcugcccguc     2700

ccggugaacg agcuccccca cggcuggaag gccuggggga agagccacuu cgugcgcgcg     2760

gccaagacca acaacuccuu cgucguggac ggcgacaccc ugaaggagug cccccugaag     2820

caccgggccu ggaacagcuu ccucguggag gaccacgggu ucggcgucuu ccacacgucc     2880

guguggcuga aggugcgcga ggacuacagc cuggagugcg accccgccgu caucggcacc     2940

gccgugaagg ggaaggaggc ggugcacucc gaccucggcu acuggaucga gagcgagaag     3000

aacgacaccu ggcggcugaa gcgcgcccac cugaucgaga ugaagaccug cgaguggccg     3060

aagucccaca cgcucuggac cgacgggauc gaggagagcg accugaucau ccccaagucc     3120

cuggccggcc cccucagcca ccacaacacc cgggaggggu accgcaccca gaugaagggc     3180

cccuggcacu ccgaggagcu ggagauccgg uucgaggagu gcccgggcac gaagguccac     3240

guggaggaga ccugcgggac ccgcggcccc agccugcggu ccaccacggc cagcgggcgc     3300

gucaucgagg aguggugcug ccgggagugc accaugcccc cgcucuccuu ccgcgccaag     3360

gacggcugcu gguacggcau ggagauccgg ccgcgcaagg agcccgagag caaccuggug     3420

cgguccaugg ugaccgcg                                                   3438


<210>  416
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-prME-NS1

<400>  416
augaagaacc ccaagaagaa gagcggcggg uuccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg uguccccguu cgggggccuc aagcggcugc ccgccgggcu gcuccugggc      120

cacggcccca uccgcauggu gcuggcgauc cucgccuucc ugcgguucac cgccaucaag      180

cccagccugg ggcucaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcugcgga ucaucaacgc ccgcaaggag      300

aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc      360

auggcggccg aggucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      420

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      480

auggaccugg ggcacacgug cgacgcgacc auguccuacg agugccccau gcucgacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca cgagcaccug ggucguguac      600

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      660

agccacucca cgcgcaagcu gcagacccgg agccagaccu ggcucgaguc ccgcgaguac      720

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      780

gcggccgcca ucgccuggcu ccuggggagc uccacgagcc agaaggugau cuaccuggug      840

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      900

uucguggagg ggauguccgg cggcaccugg guggacgucg ugcuggagca cgggggcugc      960

gugaccguca uggcccagga caagcccacc guggacaucg agcucgugac gaccaccguc     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggccu ccaucagcga cauggccucc     1080

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacgcaguac     1140

gucugcaagc gcacccuggu ggaccggggc ugggggaacg gcugcggccu cuucgggaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc     1260

cagccggaga accuggagua ccgcaucaug cucuccgucc acggcagcca gcacuccggg     1320

augaucguga acgacacggg ccacgagacc gacgagaacc gggccaaggu ggagaucacc     1380

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1440

gagccccgga cgggccugga cuucagcgac cuguacuacc ucaccaugaa caacaagcac     1500

uggcuggucc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1560

accgggaccc cgcacuggaa caacaaggag gcccucgugg aguucaagga cgcgcacgcc     1620

aagcgccaga cgguggucgu gcugggcucc caggaggggg ccgugcacac cgcccuggcc     1680

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1740

ugccgccuga agauggacaa gcuccggcug aaggggguca gcuacucccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgcggag acgcuccacg gcaccgugac cguggagguc     1860

caguacgccg ggaccgacgg cccgugcaag gugcccgccc agauggccgu ggacaugcag     1920

acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg ugaucaccga gagcacggag     1980

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     2040

gucggggaga agaagaucac ccaccacugg caccggagcg gcuccaccau cgggaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc ugggggacac ggcgugggac     2160

uucggcagcg ucggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     2220

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacc     2280

cugcugaugu ggcucgggcu gaacgccaag aacggcucca ucagccugau gugccucgcg     2340

cugggcggcg ugcugaucuu ccucuccacc gccgugagcg ccgacgucgg gugcuccgug     2400

gacuucagca agaaggagac ccgcugcggg acgggcgugu ucgucuacaa cgacguggag     2460

gccuggcggg accgcuacaa guaccacccc gacuccccgc gccggcuggc cgcggccgug     2520

aagcaggccu gggaggacgg gaucugcggc aucagcuccg ucagccgcau ggagaacauc     2580

auguggcgga gcguggaggg ggagcugaac gccauccucg aggagaacgg cgugcagcug     2640

accgucgugg ugggcuccgu caagaacccg auguggcgcg ggccccagcg gcugcccgug     2700

ccggugaacg agcuccccca cggcuggaag gcguggggga agagcuacuu cguccgcgcc     2760

gccaagacca acaacuccuu cgugguggac ggcgacaccc ugaaggagug cccccugaag     2820

caccgggccu ggaacagcuu ccucgucgag gaccacgggu ucggcguguu ccacacgucc     2880

guguggcuga agguccgcga ggacuacagc cuggagugcg accccgccgu gaucggcacc     2940

gcggugaagg ggaaggaggc cguccacucc gaccucggcu acuggaucga gagcgagaag     3000

aacgacaccu ggcggcugaa gcgcgcccac cugaucgaga ugaagaccug cgaguggccg     3060

aagucccaca cgcucuggac cgacgggauc gaggagagcg accugaucau ccccaagucc     3120

cuggccggcc cccucagcca ccacaacacc cgggaggggu accgcaccca gaugaagggc     3180

cccuggcacu ccgaggagcu ggagauccgg uucgaggagu gcccgggcac gaaggugcac     3240

gucgaggaga ccugcgggac ccgcggcccc agccugcggu ccaccacggc cagcgggcgc     3300

gugaucgagg aguggugcug ccgggagugc accaugccgc cccucuccuu ccgcgcgaag     3360

gacggcugcu gguacggcau ggagauccgg ccgcgcaagg agcccgagag caaccuggug     3420

cgguccaugg ucaccgcc                                                   3438


<210>  417
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-prME-NS1

<400>  417
augaagaacc ccaaggagga gauccgccgg auccgcaucg ugaacaugcu gaagcggggc       60

gucgcccgcg ugaacccgcu cgggggccug aagcggcugc ccgccgggcu ccugcugggc      120

cacgggccca uccgcauggu gcucgcgauc cuggccuucc ugcgguucac cgccaucaag      180

cccagccucg gccugaucaa ccgcuggggc uccgucggga agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcg augcuccgga ucaucaacgc ccgcaaggag      300

cggaagcgcc ggggcgccga caccagcauc gggaucaucg gccugcugcu cacgaccgcc      360

auggcggccg agaucacccg ccgggggucc gccuacuaca uguaccugga ccgcagcgac      420

gccggcaagg ccaucuccuu cgcgaccacg cugggcguga acaagugcca cgugcagauc      480

auggaccucg ggcacaugug cgacgccacc augagcuacg agugcccgau gcuggacgag      540

ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca ccuccacgug gguggucuac      600

gggaccugcc accacaagaa gggcgaggcc cggcgcagcc ggcgcgccgu gacccugccc      660

ucccacagca cccggaagcu ccagacgcgc ucccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugaucaaggu ggagaacugg aucuuccgga accccggcuu cgcccucguc      780

gcgguggcca ucgccuggcu gcuggggucc agcaccuccc agaaggugau cuaccucguc      840

augauccugc ugaucgcccc ggccuacagc auccgcugca ucggcguguc caaccgggac      900

uucguggagg ggaugagcgg cgggacgugg gucgacgugg ugcucgagca cggcggcugc      960

gucaccguga uggcgcagga caagcccacc guggacaucg agcuggucac cacgaccgug     1020

uccaacaugg ccgagguccg cagcuacugc uacgaggcca gcaucuccga cauggccagc     1080

gacucccggu gccccaccca gggggaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacgcucgu ggaccggggc ugggggaacg gcugcgggcu guucggcaag     1200

ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga agaugaccgg gaaguccauc     1260

cagcccgaga accucgagua ccgcaucaug cugagcgugc acggcuccca gcacagcggg     1320

augaucggcu acgagacgga cgaggaccgg gccaaggugg aggucacccc gaacuccccg     1380

cgcgccgagg ccacccuggg cggguucggc agccucgggc uggacugcga gccccggacc     1440

ggccuggacu ucuccgaccu cuacuaccug acgaugaaca acaagcacug gcuggugcac     1500

aaggaguggu uccacgacau cccccucccg uggcacgcgg gggccgacac cggcaccccg     1560

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1620

gucguggugc ugggcagcca ggagggggcg guccacacgg cccuggccgg cgcccucgag     1680

gccgagaugg acggggcgaa gggccggcug uucuccgggc accugaagug ccgccucaag     1740

auggacaagc ugcggcugaa gggcgugagc uacucccucu gcaccgccgc cuucaccuuc     1800

accaaggugc ccgccgagac ccugcacggc acggucaccg uggaggugca guacgccggg     1860

accgacggcc ccugcaagau cccgguccag auggcggugg acaugcagac ccugacgccc     1920

guggggcgcc ucaucaccgc caaccccguc aucaccgaga gcaccgagaa cuccaagaug     1980

augcuggagc uggacccgcc guucggcgac agcuacaucg ugaucggggu gggcgacaag     2040

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     2100

guccgcggcg cgaagcggau ggccgugcuc ggggacaccg ccugggacuu cggcagcgug     2160

ggcggcgucu ucaacucccu ggggaagggg auccaccaga ucuucggcgc cgccuucaag     2220

agccuguucg ggggcauguc cugguucagc cagauccuca ucgggacgcu gcuggugugg     2280

cucggccuga acaccaagaa cggcuccauc agccugaccu gccucgcgcu gggcggcgug     2340

augaucuucc uguccaccgc cgucagcgcc gacguggggu gcuccgugga cuucagcaag     2400

aaggagacgc gcugcgggac cggcgucuuc aucuacaacg acguggaggc cuggcgcgac     2460

cgguacaagu accaccccga cuccccgcgc cggcucgccg cggccgugaa gcaggccugg     2520

gaggagggga ucugcggcau cagcuccguc agccgcaugg agaacaucau guggaagucc     2580

guggaggggg agcugaacgc cauccuggag gagaacggcg ugcagcucac cgucguggug     2640

gggagcguca agaaccccau guggcggggc ccgcagcgcc ugcccgugcc cgugaacgag     2700

cugccgcacg gcuggaaggc cugggggaag agcuacuucg uccgggcggc caagaccaac     2760

aacuccuucg ugguggacgg cgacacgcuc aaggagugcc cccuggagca ccgcgccugg     2820

aacagcuucc uggucgagga ccacggguuc ggcguguucc acaccuccgu guggcucaag     2880

guccgggagg acuacagccu ggagugcgac cccgccguga ucgggaccgc ggucaagggc     2940

cgcgaggccg cccacuccga ccugggcuac uggaucgaga gcgagaagaa cgacaccugg     3000

cggcucaagc gcgcccaccu gaucgagaug aagacgugcg aguggcccaa gucccacacc     3060

cuguggaccg acggggugga ggagagcgac cucaucaucc cgaagucccu ggccggcccc     3120

cugagccacc acaacacccg ggagggguac cgcacccagg ugaagggccc cuggcacucc     3180

gaggagcucg agauccgguu cgaggagugc cccgggacca aggucuacgu ggaggagacc     3240

ugcggcaccc gcggcccgag ccugcggucc acgaccgcga gcgggcgcgu gaucgaggag     3300

uggugcugcc gggagugcac caugccgccc cuguccuucc gcgccaagga cggcugcugg     3360

uacgggaugg agauccggcc ccgcaaggag ccggagagca accucguccg cuccauggug     3420

accgcc                                                                3426


<210>  418
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C-prME-NS1

<400>  418
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gcggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc      360

auggccgccg aggugacccg ccgcggcagc gccuacuaca uguaccugga ccgcaacgac      420

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      480

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugauccgcgu ggagaacugg aucuuccgca accccggcuu cgcccuggcc      780

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucguga acgacaccgg ccacgagacc gacgagaacc gcgccaaggu ggagaucacc     1380

cccaacagcc cccgcgccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1440

gagccccgca ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1560

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1620

aagcgccaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1680

ggcgcccugg aggccgagau ggacggcgcc aagggccgcc ugagcagcgg ccaccugaag     1740

ugccgccuga agauggacaa gcugcgccug aagggcguga gcuacagccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1860

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1920

acccugaccc ccgugggccg ccugaucacc gccaaccccg ugaucaccga gagcaccgag     1980

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     2040

gugggcgaga agaagaucac ccaccacugg caccgcagcg gcagcaccau cggcaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc ugggcgacac cgccugggac     2160

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     2220

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     2280

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2340

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     2400

gacuucagca agaaggagac ccgcugcggc accggcgugu ucguguacaa cgacguggag     2460

gccuggcgcg accgcuacaa guaccacccc gacagccccc gccgccuggc cgccgccgug     2520

aagcaggccu gggaggacgg caucugcggc aucagcagcg ugagccgcau ggagaacauc     2580

auguggcgca gcguggaggg cgagcugaac gccauccugg aggagaacgg cgugcagcug     2640

accguggugg ugggcagcgu gaagaacccc auguggcgcg gcccccagcg ccugcccgug     2700

cccgugaacg agcugcccca cggcuggaag gccuggggca agagccacuu cgugcgcgcc     2760

gccaagacca acaacagcuu cgugguggac ggcgacaccc ugaaggagug cccccugaag     2820

caccgcgccu ggaacagcuu ccugguggag gaccacggcu ucggcguguu ccacaccagc     2880

guguggcuga aggugcgcga ggacuacagc cuggagugcg accccgccgu gaucggcacc     2940

gccgugaagg gcaaggaggc cgugcacagc gaccugggcu acuggaucga gagcgagaag     3000

aacgacaccu ggcgccugaa gcgcgcccac cugaucgaga ugaagaccug cgaguggccc     3060

aagagccaca cccuguggac cgacggcauc gaggagagcg accugaucau ccccaagagc     3120

cuggccggcc cccugagcca ccacaacacc cgcgagggcu accgcaccca gaugaagggc     3180

cccuggcaca gcgaggagcu ggagauccgc uucgaggagu gccccggcac caaggugcac     3240

guggaggaga ccugcggcac ccgcggcccc agccugcgca gcaccaccgc cagcggccgc     3300

gugaucgagg aguggugcug ccgcgagugc accaugcccc cccugagcuu ccgcgccaag     3360

gacggcugcu gguacggcau ggagauccgc ccccgcaagg agcccgagag caaccuggug     3420

cgcagcaugg ugaccgcc                                                   3438


<210>  419
<211>  3438
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C-prME-NS1

<400>  419
augaagaacc ccaagaagaa gagcggcggc uuccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugagccccuu cggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

aagaagcgcc gcggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc      360

auggccgccg aggugacccg ccgcggcagc gccuacuaca uguaccugga ccgcaacgac      420

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      480

auggaccugg gccacaccug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugauccgcgu ggagaacugg aucuuccgca accccggcuu cgcccuggcc      780

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucguga acgacaccgg ccacgagacc gacgagaacc gcgccaaggu ggagaucacc     1380

cccaacagcc cccgcgccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1440

gagccccgca ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1500

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1560

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1620

aagcgccaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1680

ggcgcccugg aggccgagau ggacggcgcc aagggccgcc ugagcagcgg ccaccugaag     1740

ugccgccuga agauggacaa gcugcgccug aagggcguga gcuacagccu gugcaccgcc     1800

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1860

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1920

acccugaccc ccgugggccg ccugaucacc gccaaccccg ugaucaccga gagcaccgag     1980

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     2040

gugggcgaga agaagaucac ccaccacugg caccgcagcg gcagcaccau cggcaaggcc     2100

uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc ugggcgacac cgccugggac     2160

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     2220

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     2280

cugcugaugu ggcugggccu gaacgccaag aacggcagca ucagccugau gugccuggcc     2340

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     2400

gacuucagca agaaggagac ccgcugcggc accggcgugu ucguguacaa cgacguggag     2460

gccuggcgcg accgcuacaa guaccacccc gacagccccc gccgccuggc cgccgccgug     2520

aagcaggccu gggaggacgg caucugcggc aucagcagcg ugagccgcau ggagaacauc     2580

auguggcgca gcguggaggg cgagcugaac gccauccugg aggagaacgg cgugcagcug     2640

accguggugg ugggcagcgu gaagaacccc auguggcgcg gcccccagcg ccugcccgug     2700

cccgugaacg agcugcccca cggcuggaag gccuggggca agagcuacuu cgugcgcgcc     2760

gccaagacca acaacagcuu cgugguggac ggcgacaccc ugaaggagug cccccugaag     2820

caccgcgccu ggaacagcuu ccugguggag gaccacggcu ucggcguguu ccacaccagc     2880

guguggcuga aggugcgcga ggacuacagc cuggagugcg accccgccgu gaucggcacc     2940

gccgugaagg gcaaggaggc cgugcacagc gaccugggcu acuggaucga gagcgagaag     3000

aacgacaccu ggcgccugaa gcgcgcccac cugaucgaga ugaagaccug cgaguggccc     3060

aagagccaca cccuguggac cgacggcauc gaggagagcg accugaucau ccccaagagc     3120

cuggccggcc cccugagcca ccacaacacc cgcgagggcu accgcaccca gaugaagggc     3180

cccuggcaca gcgaggagcu ggagauccgc uucgaggagu gccccggcac caaggugcac     3240

guggaggaga ccugcggcac ccgcggcccc agccugcgca gcaccaccgc cagcggccgc     3300

gugaucgagg aguggugcug ccgcgagugc accaugcccc cccugagcuu ccgcgccaag     3360

gacggcugcu gguacggcau ggagauccgc ccccgcaagg agcccgagag caaccuggug     3420

cgcagcaugg ugaccgcc                                                   3438


<210>  420
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C-prME-NS1

<400>  420
augaagaacc ccaaggagga gauccgccgc auccgcaucg ugaacaugcu gaagcgcggc       60

guggcccgcg ugaacccccu gggcggccug aagcgccugc ccgccggccu gcugcugggc      120

cacggcccca uccgcauggu gcuggccauc cuggccuucc ugcgcuucac cgccaucaag      180

cccagccugg gccugaucaa ccgcuggggc agcgugggca agaaggaggc cauggagauc      240

aucaagaagu ucaagaagga ccuggccgcc augcugcgca ucaucaacgc ccgcaaggag      300

cgcaagcgcc gcggcgccga caccagcauc ggcaucaucg gccugcugcu gaccaccgcc      360

auggccgccg agaucacccg ccgcggcagc gccuacuaca uguaccugga ccgcagcgac      420

gccggcaagg ccaucagcuu cgccaccacc cugggcguga acaagugcca cgugcagauc      480

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      540

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      600

ggcaccugcc accacaagaa gggcgaggcc cgccgcagcc gccgcgccgu gacccugccc      660

agccacagca cccgcaagcu gcagacccgc agccagaccu ggcuggagag ccgcgaguac      720

accaagcacc ugaucaaggu ggagaacugg aucuuccgca accccggcuu cgcccuggug      780

gccguggcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      840

augauccugc ugaucgcccc cgccuacagc auccgcugca ucggcgugag caaccgcgac      900

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      960

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug     1020

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc     1080

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac     1140

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag     1200

ggcagccugg ugaccugcgc caaguucacc ugcagcaaga agaugaccgg caagagcauc     1260

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc     1320

augaucggcu acgagaccga cgaggaccgc gccaaggugg aggugacccc caacagcccc     1380

cgcgccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gccccgcacc     1440

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1500

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1560

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1620

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1680

gccgagaugg acggcgccaa gggccgccug uucagcggcc accugaagug ccgccugaag     1740

auggacaagc ugcgccugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1800

accaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1860

accgacggcc ccugcaagau ccccgugcag auggccgugg acaugcagac ccugaccccc     1920

gugggccgcc ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1980

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     2040

aagaucaccc accacuggca ccgcagcggc agcaccaucg gcaaggccuu cgaggccacc     2100

gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     2160

ggcggcgugu ucaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     2220

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcuggugugg     2280

cugggccuga acaccaagaa cggcagcauc agccugaccu gccuggcccu gggcggcgug     2340

augaucuucc ugagcaccgc cgugagcgcc gacgugggcu gcagcgugga cuucagcaag     2400

aaggagaccc gcugcggcac cggcguguuc aucuacaacg acguggaggc cuggcgcgac     2460

cgcuacaagu accaccccga cagcccccgc cgccuggccg ccgccgugaa gcaggccugg     2520

gaggagggca ucugcggcau cagcagcgug agccgcaugg agaacaucau guggaagagc     2580

guggagggcg agcugaacgc cauccuggag gagaacggcg ugcagcugac cgugguggug     2640

ggcagcguga agaaccccau guggcgcggc ccccagcgcc ugcccgugcc cgugaacgag     2700

cugccccacg gcuggaaggc cuggggcaag agcuacuucg ugcgcgccgc caagaccaac     2760

aacagcuucg ugguggacgg cgacacccug aaggagugcc cccuggagca ccgcgccugg     2820

aacagcuucc ugguggagga ccacggcuuc ggcguguucc acaccagcgu guggcugaag     2880

gugcgcgagg acuacagccu ggagugcgac cccgccguga ucggcaccgc cgugaagggc     2940

cgcgaggccg cccacagcga ccugggcuac uggaucgaga gcgagaagaa cgacaccugg     3000

cgccugaagc gcgcccaccu gaucgagaug aagaccugcg aguggcccaa gagccacacc     3060

cuguggaccg acggcgugga ggagagcgac cugaucaucc ccaagagccu ggccggcccc     3120

cugagccacc acaacacccg cgagggcuac cgcacccagg ugaagggccc cuggcacagc     3180

gaggagcugg agauccgcuu cgaggagugc cccggcacca agguguacgu ggaggagacc     3240

ugcggcaccc gcggccccag ccugcgcagc accaccgcca gcggccgcgu gaucgaggag     3300

uggugcugcc gcgagugcac caugcccccc cugagcuucc gcgccaagga cggcugcugg     3360

uacggcaugg agauccgccc ccgcaaggag cccgagagca accuggugcg cagcauggug     3420

accgcc                                                                3426


<210>  421
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS-prME

<400>  421

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              


Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala 
    690 


<210>  422
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS-prME

<400>  422

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              


Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Met Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala 
    690 


<210>  423
<211>  686
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS-prME

<400>  423

Gly Ala Asp Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly 
        35                  40                  45              


Val Asn Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr 
            340                 345                 350         


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
        355                 360                 365             


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
    370                 375                 380                 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
385                 390                 395                 400 


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
                405                 410                 415     


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
            420                 425                 430         


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
        435                 440                 445             


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
    450                 455                 460                 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
465                 470                 475                 480 


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
                485                 490                 495     


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
            500                 505                 510         


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala 
        515                 520                 525             


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
    530                 535                 540                 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
545                 550                 555                 560 


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
                565                 570                 575     


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
            580                 585                 590         


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
        595                 600                 605             


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
    610                 615                 620                 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
625                 630                 635                 640 


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
                645                 650                 655     


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
            660                 665                 670         


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685     


<210>  424
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS-prME

<400>  424
ggcgcagaua cuagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag       60

gucacuagac gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc      120

auaucuuuuc caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga      180

cacaugugug augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca      240

gaugacgucg auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau      300

cacaaaaaag gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu      360

aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug      420

auuagagucg aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc      480

gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu      600

augucaggug ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug      660

gcacaggaca aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg      720

gagguaagau ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acguuagugg acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug      900

acaugcgcua aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau      960

cuggaguacc ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau     1020

gacacaggac augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca     1080

agagccgaag ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca     1140

ggccuugacu uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac     1200

aaggaguggu uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu     1320

gucgugguuc uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag     1380

gcugagaugg auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa     1440

auggauaaac uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc     1500

accaagaucc cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg     1560

acagauggac cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca     1620

guugggaggu ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug     1680

augcuggaac uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag     1740

aagaucaccc accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu     1800

gugagaggug ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa     1920

ucauuguuug gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg     1980

uugggucuga acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug     2040

uugaucuucu uauccacagc cgucucugcu                                      2070


<210>  425
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS-prME

<400>  425
ggcgcagaua cuagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag       60

gucacuagac gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc      120

auaucuuuuc caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga      180

cacacgugug augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca      240

gaugacgucg auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau      300

cacaaaaaag gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu      360

aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug      420

auuagagucg aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc      480

gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu      600

augucaggug ggacuugggu ugauguuguc uuggaacaug gagguugugu cacuguaaug      660

gcacaggaca aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg      720

gagguaagau ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acguuagugg acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagccuggug      900

acaugcgcua aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau      960

cuggaguacc ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau     1020

gacacaggac augaaacuga ugagaauaga gcgaaaguug agauaacgcc caauucacca     1080

agagccgaag ccacccuggg gggguuugga agccuaggac uugauuguga accgaggaca     1140

ggccuugacu uuucagauuu guauuacuug acuaugaaua acaagcacug gcugguucac     1200

aaggaguggu uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu     1320

gucgugguuc uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag     1380

gcugagaugg auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa     1440

auggauaaac uuagauugaa gggcguguca uacuccuugu guacugcagc guucacauuc     1500

accaagaucc cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg     1560

acagauggac cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca     1620

guugggaggu ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug     1680

augcuggaac uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag     1740

aagaucaccc accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu     1800

gugagaggug ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa ucuuuggagc agcuuucaaa     1920

ucauuguuug gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg     1980

uugggucuga acgcaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug     2040

uugaucuucu uauccacagc cgucucugcu                                      2070


<210>  426
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS-prME

<400>  426
ggcgcagaca ccagcaucgg aaucauuggc cuccugcuga cuacagccau ggcagcagag       60

aucacuagac gcgggagugc auacuacaug uacuuggaua ggagcgaugc cgggaaggcc      120

auuucguuug cuaccacauu gggagugaac aagugccacg uacagaucau ggaccucggg      180

cacaugugug acgccaccau gaguuaugag ugcccuaugc uggaugaggg aguggaacca      240

gaugaugucg auugcuggug caacacgaca ucaacuuggg uuguguacgg aaccugucau      300

cacaaaaaag gugaggcacg gcgaucuaga agagccguga cgcucccuuc ucacucuaca      360

aggaaguugc aaacgcgguc gcagaccugg uuagaaucaa gagaauacac gaagcacuug      420

aucaagguug aaaacuggau auucaggaac cccggguuug cgcuaguggc cguugccauu      480

gccuggcuuu ugggaagcuc gacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacaguau caggugcauu ggagucagca auagagacuu cguggagggc      600

augucaggug ggaccugggu ugauguuguc uuggaacaug gaggcugcgu uaccgugaug      660

gcacaggaca agccaacagu cgacauagag uuggucacga cgacgguuag uaacauggcc      720

gagguaagau ccuauugcua cgaggcaucg auaucggaca uggcuucgga cagucguugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acauuagugg acagagguug gggaaacggu uguggacuuu uuggcaaagg gagcuuggug      900

acaugugcca aguuuacgug uucuaagaag augaccggga agagcauuca accggaaaau      960

cuggaguauc ggauaaugcu aucagugcau ggcucccagc auagcgggau gauuggauau     1020

gaaacugacg aagauagagc gaaagucgag guuacgccua auucaccaag agcggaagca     1080

accuugggag gcuuuggaag cuuaggacuu gacugugaac caaggacagg ccuugacuuu     1140

ucagaucugu auuaccugac caugaacaau aagcauuggu uggugcacaa agagugguuu     1200

caugacaucc cauugccuug gcaugcuggg gcagacaccg gaacuccaca cuggaacaac     1260

aaagaggcau ugguagaauu caaggaugcc cacgccaaga ggcaaaccgu cgucguucug     1320

gggagccagg aaggagccgu ucacacggcu cucgcuggag cucuagaggc ugagauggau     1380

ggugcaaagg gaaggcuguu cucuggccau uugaaaugcc gccuaaaaau ggacaagcuu     1440

agauugaagg gcgugucaua uuccuugugc acugcggcau ucacauucac caagguccca     1500

gcugaaacac ugcauggaac agucacagug gaggugcagu augcagggac agauggaccc     1560

ugcaagaucc caguccagau ggcgguggac augcagaccc ugaccccagu uggaaggcug     1620

auaaccgcca accccgugau uacugaaagc acugagaacu caaagaugau guuggagcuu     1680

gacccaccau uuggggauuc uuacauuguc auaggaguug gggacaagaa aaucacccac     1740

cacuggcaua ggagugguag caccaucgga aaggcauuug aggccacugu gagaggcgcc     1800

aagagaaugg caguccuggg ggauacagcc ugggacuucg gaucagucgg ggguguguuc     1860

aacucacugg guaagggcau ucaccagauu uuuggagcag ccuucaaauc acuguuugga     1920

ggaauguccu gguucucaca gauccucaua ggcacgcugc uagugugguu agguuugaac     1980

acaaagaaug gaucuaucuc ccucacaugc uuggcccugg ggggagugau gaucuuccuc     2040

uccacggcug uuucugcu                                                   2058


<210>  427
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS-prME

<400>  427
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag       60

gucacccggc gcggcuccgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc      120

aucagcuucc cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg      180

cacaugugcg acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac      300

cacaagaagg gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc      360

cgcaagcugc agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug      420

auccgggucg agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc      480

gccuggcucc uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug      540

aucgcccccg cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg      600

auguccggcg gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc      720

gagguccgca gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc      780

cccacccagg gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc      840

acgcuggucg accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug      900

accugcgcca aguucgccug cuccaagaag augaccggga agagcaucca gccggagaac      960

cuggaguacc gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac     1020

gacaccggcc acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc     1080

cgcgcggagg ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc     1140

ggccuggacu ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc     1320

gugguggucc ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag     1380

gccgagaugg acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg     1560

accgacggcc cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc     1620

guggggcgcc ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag     1740

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

guccgcggcg ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug     1860

ggcggggccc ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg     1980

cucgggcuga acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc     2040

cugaucuucc ucuccaccgc ggugagcgcc                                      2070


<210>  428
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS-prME

<400>  428
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag       60

gucacccggc gcggcuccgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc      120

aucagcuucc cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg      180

cacacgugcg acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacg agcaccuggg ucguguacgg cacgugccac      300

cacaagaagg gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacg      360

cgcaagcugc agacccggag ccagaccugg cucgaguccc gcgaguacac caagcaccug      420

auccgggucg agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc      480

gccuggcucc uggggagcuc cacgagccag aaggugaucu accuggugau gauccuccug      540

aucgcccccg cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg      600

auguccggcg gcaccugggu ggacgucgug cuggagcacg ggggcugcgu gaccgucaug      660

gcccaggaca agcccaccgu ggacaucgag cucgugacga ccaccgucag caacauggcc      720

gaggugcgca gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc      780

cccacccagg gggaggcgua ccuggacaag caguccgaca cgcaguacgu cugcaagcgc      840

acccuggugg accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug      900

accugcgcca aguucgccug cuccaagaag augaccggga agagcaucca gccggagaac      960

cuggaguacc gcaucaugcu cuccguccac ggcagccagc acuccgggau gaucgugaac     1020

gacacgggcc acgagaccga cgagaaccgg gccaaggugg agaucacccc caacagcccc     1080

cgcgcggagg ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacg     1140

ggccuggacu ucagcgaccu guacuaccuc accaugaaca acaagcacug gcugguccac     1200

aaggaguggu uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg     1260

cacuggaaca acaaggaggc ccucguggag uucaaggacg cgcacgccaa gcgccagacg     1320

guggucgugc ugggcuccca ggagggggcc gugcacaccg cccuggccgg cgcgcuggag     1380

gccgagaugg acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggggucagc uacucccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgcggagac gcuccacggc accgugaccg uggaggucca guacgccggg     1560

accgacggcc cgugcaaggu gcccgcccag auggccgugg acaugcagac gcugaccccc     1620

gucgggcgcc ugaucaccgc gaaccccgug aucaccgaga gcacggagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac uccuacaucg ugaucggcgu cggggagaag     1740

aagaucaccc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

gugcgcggcg ccaagcggau ggccgugcug ggggacacgg cgugggacuu cggcagcguc     1860

ggcggggccc ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucggcacccu gcugaugugg     1980

cucgggcuga acgccaagaa cggcuccauc agccugaugu gccucgcgcu gggcggcgug     2040

cugaucuucc ucuccaccgc cgugagcgcc                                      2070


<210>  429
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS-prME

<400>  429
ggcgccgaca ccagcaucgg gaucaucggc cugcugcuca cgaccgccau ggcggccgag       60

aucacccgcc ggggguccgc cuacuacaug uaccuggacc gcagcgacgc cggcaaggcc      120

aucuccuucg cgaccacgcu gggcgugaac aagugccacg ugcagaucau ggaccucggg      180

cacaugugcg acgccaccau gagcuacgag ugcccgaugc uggacgaggg cgucgagccc      240

gacgacgugg acugcuggug caacaccacc uccacguggg uggucuacgg gaccugccac      300

cacaagaagg gcgaggcccg gcgcagccgg cgcgccguga cccugcccuc ccacagcacc      360

cggaagcucc agacgcgcuc ccagaccugg cuggagagcc gcgaguacac caagcaccug      420

aucaaggugg agaacuggau cuuccggaac cccggcuucg cccucgucgc gguggccauc      480

gccuggcugc ugggguccag caccucccag aaggugaucu accucgucau gauccugcug      540

aucgccccgg ccuacagcau ccgcugcauc ggcgugucca accgggacuu cguggagggg      600

augagcggcg ggacgugggu cgacguggug cucgagcacg gcggcugcgu caccgugaug      660

gcgcaggaca agcccaccgu ggacaucgag cuggucacca cgaccguguc caacauggcc      720

gagguccgca gcuacugcua cgaggccagc aucuccgaca uggccagcga cucccggugc      780

cccacccagg gggaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagcgc      840

acgcucgugg accggggcug ggggaacggc ugcgggcugu ucggcaaggg cucccugguc      900

acgugcgcga aguucacgug cagcaagaag augaccggga aguccaucca gcccgagaac      960

cucgaguacc gcaucaugcu gagcgugcac ggcucccagc acagcgggau gaucggcuac     1020

gagacggacg aggaccgggc caagguggag gucaccccga acuccccgcg cgccgaggcc     1080

acccugggcg gguucggcag ccucgggcug gacugcgagc cccggaccgg ccuggacuuc     1140

uccgaccucu acuaccugac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1200

cacgacaucc cccucccgug gcacgcgggg gccgacaccg gcaccccgca cuggaacaac     1260

aaggaggccc ugguggaguu caaggacgcc cacgccaagc gccagaccgu cguggugcug     1320

ggcagccagg agggggcggu ccacacggcc cuggccggcg cccucgaggc cgagauggac     1380

ggggcgaagg gccggcuguu cuccgggcac cugaagugcc gccucaagau ggacaagcug     1440

cggcugaagg gcgugagcua cucccucugc accgccgccu ucaccuucac caaggugccc     1500

gccgagaccc ugcacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccc     1560

ugcaagaucc cgguccagau ggcgguggac augcagaccc ugacgcccgu ggggcgccuc     1620

aucaccgcca accccgucau caccgagagc accgagaacu ccaagaugau gcuggagcug     1680

gacccgccgu ucggcgacag cuacaucgug aucggggugg gcgacaagaa gaucacgcac     1740

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcg     1800

aagcggaugg ccgugcucgg ggacaccgcc ugggacuucg gcagcguggg cggcgucuuc     1860

aacucccugg ggaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1920

ggcauguccu gguucagcca gauccucauc gggacgcugc ugguguggcu cggccugaac     1980

accaagaacg gcuccaucag ccugaccugc cucgcgcugg gcggcgugau gaucuuccug     2040

uccaccgccg ucagcgcc                                                   2058


<210>  430
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS-prME

<400>  430
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag       60

gugacccgcc gcggcagcgc cuacuacaug uaccuggacc gcaacgacgc cggcgaggcc      120

aucagcuucc ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcagccgc cgcgccguga cccugcccag ccacagcacc      360

cgcaagcugc agacccgcag ccagaccugg cuggagagcc gcgaguacac caagcaccug      420

auccgcgugg agaacuggau cuuccgcaac cccggcuucg cccuggccgc cgccgccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau ccgcugcauc ggcgugagca accgcgacuu cguggagggc      600

augagcggcg gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugcgca gcuacugcua cgaggccagc aucagcgaca uggccagcga cagccgcugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagcgc      840

acccuggugg accgcggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguacc gcaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaccggcc acgagaccga cgagaaccgc gccaaggugg agaucacccc caacagcccc     1080

cgcgccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gccccgcacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggccgccug agcagcggcc accugaagug ccgccugaag     1440

auggacaagc ugcgccugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggccgcc ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag     1740

aagaucaccc accacuggca ccgcagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgccc ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug     2040

cugaucuucc ugagcaccgc cgugagcgcc                                      2070


<210>  431
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS-prME

<400>  431
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag       60

gugacccgcc gcggcagcgc cuacuacaug uaccuggacc gcaacgacgc cggcgaggcc      120

aucagcuucc ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc      180

cacaccugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcagccgc cgcgccguga cccugcccag ccacagcacc      360

cgcaagcugc agacccgcag ccagaccugg cuggagagcc gcgaguacac caagcaccug      420

auccgcgugg agaacuggau cuuccgcaac cccggcuucg cccuggccgc cgccgccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau ccgcugcauc ggcgugagca accgcgacuu cguggagggc      600

augagcggcg gcaccugggu ggacguggug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugcgca gcuacugcua cgaggccagc aucagcgaca uggccagcga cagccgcugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagcgc      840

acccuggugg accgcggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguacc gcaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaccggcc acgagaccga cgagaaccgc gccaaggugg agaucacccc caacagcccc     1080

cgcgccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gccccgcacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggccgccug agcagcggcc accugaagug ccgccugaag     1440

auggacaagc ugcgccugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggccgcc ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag     1740

aagaucaccc accacuggca ccgcagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgccc ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg     1980

cugggccuga acgccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug     2040

cugaucuucc ugagcaccgc cgugagcgcc                                      2070


<210>  432
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS-prME

<400>  432
ggcgccgaca ccagcaucgg caucaucggc cugcugcuga ccaccgccau ggccgccgag       60

aucacccgcc gcggcagcgc cuacuacaug uaccuggacc gcagcgacgc cggcaaggcc      120

aucagcuucg ccaccacccu gggcgugaac aagugccacg ugcagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcagccgc cgcgccguga cccugcccag ccacagcacc      360

cgcaagcugc agacccgcag ccagaccugg cuggagagcc gcgaguacac caagcaccug      420

aucaaggugg agaacuggau cuuccgcaac cccggcuucg cccugguggc cguggccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau ccgcugcauc ggcgugagca accgcgacuu cguggagggc      600

augagcggcg gcaccugggu ggacguggug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugcgca gcuacugcua cgaggccagc aucagcgaca uggccagcga cagccgcugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagcgc      840

acccuggugg accgcggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucaccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguacc gcaucaugcu gagcgugcac ggcagccagc acagcggcau gaucggcuac     1020

gagaccgacg aggaccgcgc caagguggag gugaccccca acagcccccg cgccgaggcc     1080

acccugggcg gcuucggcag ccugggccug gacugcgagc cccgcaccgg ccuggacuuc     1140

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1200

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1260

aaggaggccc ugguggaguu caaggacgcc cacgccaagc gccagaccgu gguggugcug     1320

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1380

ggcgccaagg gccgccuguu cagcggccac cugaagugcc gccugaagau ggacaagcug     1440

cgccugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caaggugccc     1500

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1560

ugcaagaucc ccgugcagau ggccguggac augcagaccc ugacccccgu gggccgccug     1620

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1680

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgacaagaa gaucacccac     1740

cacuggcacc gcagcggcag caccaucggc aaggccuucg aggccaccgu gcgcggcgcc     1800

aagcgcaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcguguuc     1860

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1920

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugguguggcu gggccugaac     1980

accaagaacg gcagcaucag ccugaccugc cuggcccugg gcggcgugau gaucuuccug     2040

agcaccgccg ugagcgcc                                                   2058


<210>  433
<211>  1042
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS-prME-NS1

<400>  433

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              


Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg 
    690                 695                 700                 


Cys Gly Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp 
705                 710                 715                 720 


Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val 
                725                 730                 735     


Lys Gln Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser Ser Val Ser Arg 
            740                 745                 750         


Met Glu Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile 
        755                 760                 765             


Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys 
    770                 775                 780                 


Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu 
785                 790                 795                 800 


Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser His Phe Val Arg Ala 
                805                 810                 815     


Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu 
            820                 825                 830         


Cys Pro Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His 
        835                 840                 845             


Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp 
    850                 855                 860                 


Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly 
865                 870                 875                 880 


Lys Glu Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys 
                885                 890                 895     


Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr 
            900                 905                 910         


Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu 
        915                 920                 925             


Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His 
    930                 935                 940                 


Asn Thr Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser 
945                 950                 955                 960 


Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His 
                965                 970                 975     


Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr 
            980                 985                 990         


Ala Ser Gly Arg Val Ile Glu Glu  Trp Cys Cys Arg Glu  Cys Thr Met 
        995                 1000                 1005             


Pro Pro  Leu Ser Phe Arg Ala  Lys Asp Gly Cys Trp  Tyr Gly Met 
    1010                 1015                 1020             


Glu Ile  Arg Pro Arg Lys Glu  Pro Glu Ser Asn Leu  Val Arg Ser 
    1025                 1030                 1035             


Met Val  Thr Ala 
    1040         


<210>  434
<211>  1042
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS-prME-NS1

<400>  434

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              


Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Met Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg 
    690                 695                 700                 


Cys Gly Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp 
705                 710                 715                 720 


Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val 
                725                 730                 735     


Lys Gln Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser Ser Val Ser Arg 
            740                 745                 750         


Met Glu Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile 
        755                 760                 765             


Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys 
    770                 775                 780                 


Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu 
785                 790                 795                 800 


Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala 
                805                 810                 815     


Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu 
            820                 825                 830         


Cys Pro Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His 
        835                 840                 845             


Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp 
    850                 855                 860                 


Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly 
865                 870                 875                 880 


Lys Glu Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys 
                885                 890                 895     


Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr 
            900                 905                 910         


Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu 
        915                 920                 925             


Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His 
    930                 935                 940                 


Asn Thr Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser 
945                 950                 955                 960 


Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His 
                965                 970                 975     


Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr 
            980                 985                 990         


Ala Ser Gly Arg Val Ile Glu Glu  Trp Cys Cys Arg Glu  Cys Thr Met 
        995                 1000                 1005             


Pro Pro  Leu Ser Phe Arg Ala  Lys Asp Gly Cys Trp  Tyr Gly Met 
    1010                 1015                 1020             


Glu Ile  Arg Pro Arg Lys Glu  Pro Glu Ser Asn Leu  Val Arg Ser 
    1025                 1030                 1035             


Met Val  Thr Ala 
    1040         


<210>  435
<211>  1038
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS-prME-NS1

<400>  435

Gly Ala Asp Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly 
        35                  40                  45              


Val Asn Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr 
            340                 345                 350         


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
        355                 360                 365             


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
    370                 375                 380                 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
385                 390                 395                 400 


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
                405                 410                 415     


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
            420                 425                 430         


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
        435                 440                 445             


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
    450                 455                 460                 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
465                 470                 475                 480 


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
                485                 490                 495     


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
            500                 505                 510         


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala 
        515                 520                 525             


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
    530                 535                 540                 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
545                 550                 555                 560 


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
                565                 570                 575     


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
            580                 585                 590         


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
        595                 600                 605             


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
    610                 615                 620                 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
625                 630                 635                 640 


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
                645                 650                 655     


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
            660                 665                 670         


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
        675                 680                 685             


Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly 
    690                 695                 700                 


Val Phe Ile Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr 
705                 710                 715                 720 


His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp 
                725                 730                 735     


Glu Glu Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile 
            740                 745                 750         


Met Trp Lys Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn 
        755                 760                 765             


Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp 
    770                 775                 780                 


Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly 
785                 790                 795                 800 


Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn 
                805                 810                 815     


Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Glu 
            820                 825                 830         


His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val 
        835                 840                 845             


Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu 
    850                 855                 860                 


Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Arg Glu Ala Ala 
865                 870                 875                 880 


His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp Thr Trp 
                885                 890                 895     


Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu Trp Pro 
            900                 905                 910         


Lys Ser His Thr Leu Trp Thr Asp Gly Val Glu Glu Ser Asp Leu Ile 
        915                 920                 925             


Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr Arg Glu 
    930                 935                 940                 


Gly Tyr Arg Thr Gln Val Lys Gly Pro Trp His Ser Glu Glu Leu Glu 
945                 950                 955                 960 


Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val Tyr Val Glu Glu Thr 
                965                 970                 975     


Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser Gly Arg 
            980                 985                 990         


Val Ile Glu Glu Trp Cys Cys Arg  Glu Cys Thr Met Pro  Pro Leu Ser 
        995                 1000                 1005             


Phe Arg  Ala Lys Asp Gly Cys  Trp Tyr Gly Met Glu  Ile Arg Pro 
    1010                 1015                 1020             


Arg Lys  Glu Pro Glu Ser Asn  Leu Val Arg Ser Met  Val Thr Ala 
    1025                 1030                 1035             


<210>  436
<211>  3126
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS-prME-NS1

<400>  436
ggcgcagaua cuagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag       60

gucacuagac gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc      120

auaucuuuuc caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga      180

cacaugugug augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca      240

gaugacgucg auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau      300

cacaaaaaag gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu      360

aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug      420

auuagagucg aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc      480

gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu      600

augucaggug ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug      660

gcacaggaca aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg      720

gagguaagau ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acguuagugg acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug      900

acaugcgcua aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau      960

cuggaguacc ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau     1020

gacacaggac augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca     1080

agagccgaag ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca     1140

ggccuugacu uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac     1200

aaggaguggu uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu     1320

gucgugguuc uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag     1380

gcugagaugg auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa     1440

auggauaaac uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc     1500

accaagaucc cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg     1560

acagauggac cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca     1620

guugggaggu ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug     1680

augcuggaac uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag     1740

aagaucaccc accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu     1800

gugagaggug ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa     1920

ucauuguuug gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg     1980

uugggucuga acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug     2040

uugaucuucu uauccacagc cgucucugcu gauguggggu gcucggugga cuucucaaag     2100

aaggagacga gaugcgguac agggguguuc gucuauaacg acguugaagc cuggagggac     2160

agguacaagu accauccuga cuccccccgu agauuggcag cagcagucaa gcaagccugg     2220

gaagauggua ucugcgggau cuccucuguu ucaagaaugg aaaacaucau guggagauca     2280

guagaagggg agcucaacgc aauccuggaa gagaauggag uucaacugac ggucguugug     2340

ggaucuguaa aaaaccccau guggagaggu ccacagagau ugcccgugcc ugugaacgag     2400

cugccccacg gcuggaaggc uugggggaaa ucgcacuucg ucagagcagc aaagacaaau     2460

aacagcuuug ucguggaugg ugacacacug aaggaaugcc cacucaaaca uagagcaugg     2520

aacagcuuuc uuguggagga ucauggguuc gggguauuuc acacuagugu cuggcucaag     2580

guuagagaag auuauucauu agagugugau ccagccguua uuggaacagc uguuaaggga     2640

aaggaggcug uacacaguga ucuaggcuac uggauugaga gugagaagaa ugacacaugg     2700

aggcugaaga gggcccaucu gaucgagaug aaaacaugug aauggccaaa gucccacaca     2760

uuguggacag auggaauaga agagagugau cugaucauac ccaagucuuu agcugggcca     2820

cucagccauc acaauaccag agagggcuac aggacccaaa ugaaagggcc auggcacagu     2880

gaagagcuug aaauucgguu ugaggaaugc ccaggcacua agguccacgu ggaggaaaca     2940

uguggaacaa gaggaccauc ucugagauca accacugcaa gcggaagggu gaucgaggaa     3000

uggugcugca gggagugcac aaugccccca cugucguucc gggcuaaaga uggcuguugg     3060

uauggaaugg agauaaggcc caggaaagaa ccagaaagca acuuaguaag gucaauggug     3120

acugca                                                                3126


<210>  437
<211>  3126
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS-prME-NS1

<400>  437
ggcgcagaua cuagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag       60

gucacuagac gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc      120

auaucuuuuc caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga      180

cacacgugug augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca      240

gaugacgucg auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau      300

cacaaaaaag gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu      360

aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug      420

auuagagucg aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc      480

gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu      600

augucaggug ggacuugggu ugauguuguc uuggaacaug gagguugugu cacuguaaug      660

gcacaggaca aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg      720

gagguaagau ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acguuagugg acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagccuggug      900

acaugcgcua aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau      960

cuggaguacc ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau     1020

gacacaggac augaaacuga ugagaauaga gcgaaaguug agauaacgcc caauucacca     1080

agagccgaag ccacccuggg gggguuugga agccuaggac uugauuguga accgaggaca     1140

ggccuugacu uuucagauuu guauuacuug acuaugaaua acaagcacug gcugguucac     1200

aaggaguggu uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu     1320

gucgugguuc uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag     1380

gcugagaugg auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa     1440

auggauaaac uuagauugaa gggcguguca uacuccuugu guacugcagc guucacauuc     1500

accaagaucc cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg     1560

acagauggac cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca     1620

guugggaggu ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug     1680

augcuggaac uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag     1740

aagaucaccc accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu     1800

gugagaggug ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa ucuuuggagc agcuuucaaa     1920

ucauuguuug gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg     1980

uugggucuga acgcaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug     2040

uugaucuucu uauccacagc cgucucugcu gauguggggu gcucggugga cuucucaaag     2100

aaggagacga gaugcgguac agggguguuc gucuauaacg acguugaagc cuggagggac     2160

agguacaagu accauccuga cuccccccgu agauuggcag cagcagucaa gcaagccugg     2220

gaagauggua ucugcgggau cuccucuguu ucaagaaugg aaaacaucau guggagauca     2280

guagaagggg agcucaacgc aauccuggaa gagaauggag uucaacugac ggucguugug     2340

ggaucuguaa aaaaccccau guggagaggu ccacagagau ugcccgugcc ugugaacgag     2400

cugccccacg gcuggaaggc uugggggaaa ucguacuucg ucagagcagc aaagacaaau     2460

aacagcuuug ucguggaugg ugacacacug aaggaaugcc cacucaaaca uagagcaugg     2520

aacagcuuuc uuguggagga ucauggguuc gggguauuuc acacuagugu cuggcucaag     2580

guuagagaag auuauucauu agagugugau ccagccguua uuggaacagc uguuaaggga     2640

aaggaggcug uacacaguga ucuaggcuac uggauugaga gugagaagaa ugacacaugg     2700

aggcugaaga gggcccaucu gaucgagaug aaaacaugug aauggccaaa gucccacaca     2760

uuguggacag auggaauaga agagagugau cugaucauac ccaagucuuu agcugggcca     2820

cucagccauc acaauaccag agagggcuac aggacccaaa ugaaagggcc auggcacagu     2880

gaagagcuug aaauucgguu ugaggaaugc ccaggcacua agguccacgu ggaggaaaca     2940

uguggaacga gaggaccauc ucugagauca accacugcaa gcggaagggu gaucgaggaa     3000

uggugcugca gggagugcac aaugccccca cugucguucc gggcuaaaga uggcuguugg     3060

uauggaaugg agauaaggcc caggaaagaa ccagaaagca acuuaguaag gucaauggug     3120

acugca                                                                3126


<210>  438
<211>  3114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS-prME-NS1

<400>  438
ggcgcagaca ccagcaucgg aaucauuggc cuccugcuga cuacagccau ggcagcagag       60

aucacuagac gcgggagugc auacuacaug uacuuggaua ggagcgaugc cgggaaggcc      120

auuucguuug cuaccacauu gggagugaac aagugccacg uacagaucau ggaccucggg      180

cacaugugug acgccaccau gaguuaugag ugcccuaugc uggaugaggg aguggaacca      240

gaugaugucg auugcuggug caacacgaca ucaacuuggg uuguguacgg aaccugucau      300

cacaaaaaag gugaggcacg gcgaucuaga agagccguga cgcucccuuc ucacucuaca      360

aggaaguugc aaacgcgguc gcagaccugg uuagaaucaa gagaauacac gaagcacuug      420

aucaagguug aaaacuggau auucaggaac cccggguuug cgcuaguggc cguugccauu      480

gccuggcuuu ugggaagcuc gacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacaguau caggugcauu ggagucagca auagagacuu cguggagggc      600

augucaggug ggaccugggu ugauguuguc uuggaacaug gaggcugcgu uaccgugaug      660

gcacaggaca agccaacagu cgacauagag uuggucacga cgacgguuag uaacauggcc      720

gagguaagau ccuauugcua cgaggcaucg auaucggaca uggcuucgga cagucguugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acauuagugg acagagguug gggaaacggu uguggacuuu uuggcaaagg gagcuuggug      900

acaugugcca aguuuacgug uucuaagaag augaccggga agagcauuca accggaaaau      960

cuggaguauc ggauaaugcu aucagugcau ggcucccagc auagcgggau gauuggauau     1020

gaaacugacg aagauagagc gaaagucgag guuacgccua auucaccaag agcggaagca     1080

accuugggag gcuuuggaag cuuaggacuu gacugugaac caaggacagg ccuugacuuu     1140

ucagaucugu auuaccugac caugaacaau aagcauuggu uggugcacaa agagugguuu     1200

caugacaucc cauugccuug gcaugcuggg gcagacaccg gaacuccaca cuggaacaac     1260

aaagaggcau ugguagaauu caaggaugcc cacgccaaga ggcaaaccgu cgucguucug     1320

gggagccagg aaggagccgu ucacacggcu cucgcuggag cucuagaggc ugagauggau     1380

ggugcaaagg gaaggcuguu cucuggccau uugaaaugcc gccuaaaaau ggacaagcuu     1440

agauugaagg gcgugucaua uuccuugugc acugcggcau ucacauucac caagguccca     1500

gcugaaacac ugcauggaac agucacagug gaggugcagu augcagggac agauggaccc     1560

ugcaagaucc caguccagau ggcgguggac augcagaccc ugaccccagu uggaaggcug     1620

auaaccgcca accccgugau uacugaaagc acugagaacu caaagaugau guuggagcuu     1680

gacccaccau uuggggauuc uuacauuguc auaggaguug gggacaagaa aaucacccac     1740

cacuggcaua ggagugguag caccaucgga aaggcauuug aggccacugu gagaggcgcc     1800

aagagaaugg caguccuggg ggauacagcc ugggacuucg gaucagucgg ggguguguuc     1860

aacucacugg guaagggcau ucaccagauu uuuggagcag ccuucaaauc acuguuugga     1920

ggaauguccu gguucucaca gauccucaua ggcacgcugc uagugugguu agguuugaac     1980

acaaagaaug gaucuaucuc ccucacaugc uuggcccugg ggggagugau gaucuuccuc     2040

uccacggcug uuucugcuga cguggggugc ucaguggacu ucucaaaaaa ggaaacgaga     2100

uguggcacgg ggguauucau cuauaaugau guugaagccu ggagggaccg guacaaguac     2160

cauccugacu ccccccgcag auuggcagca gcagucaagc aggccuggga agaggggauc     2220

ugugggaucu cauccguuuc aagaauggaa aacaucaugu ggaaaucagu agaaggggag     2280

cucaaugcua uccuagagga gaauggaguu caacugacag uuguuguggg aucuguaaaa     2340

aaccccaugu ggagaggucc acaaagauug ccagugccug ugaaugagcu gccccauggc     2400

uggaaagccu gggggaaauc guauuuuguu agggcggcaa agaccaacaa caguuuuguu     2460

gucgacggug acacacugaa ggaauguccg cuugagcaca gagcauggaa uaguuuucuu     2520

guggaggauc acggguuugg agucuuccac accagugucu ggcuuaaggu cagagaagau     2580

uacucauuag aaugugaccc agccgucaua ggaacagcug uuaagggaag ggaggccgcg     2640

cacagugauc ugggcuauug gauugaaagu gaaaagaaug acacauggag gcugaagagg     2700

gcccaccuga uugagaugaa aacaugugaa uggccaaagu cucacacauu guggacagau     2760

ggaguagaag aaagugaucu uaucauaccc aagucuuuag cugguccacu cagccaccac     2820

aacaccagag aggguuacag aacccaagug aaagggccau ggcacaguga agagcuugaa     2880

auccgguuug aggaaugucc aggcaccaag guuuacgugg aggagacaug cggaacuaga     2940

ggaccaucuc ugagaucaac uacugcaagu ggaaggguca uugaggaaug gugcuguagg     3000

gaaugcacaa ugcccccacu aucguuucga gcaaaagacg gcugcuggua uggaauggag     3060

auaaggccca ggaaagaacc agagagcaac uuagugaggu caauggugac agcg           3114


<210>  439
<211>  3126
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS-prME-NS1

<400>  439
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag       60

gucacccggc gcggcuccgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc      120

aucagcuucc cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg      180

cacaugugcg acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac      300

cacaagaagg gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc      360

cgcaagcugc agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug      420

auccgggucg agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc      480

gccuggcucc uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug      540

aucgcccccg cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg      600

auguccggcg gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc      720

gagguccgca gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc      780

cccacccagg gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc      840

acgcuggucg accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug      900

accugcgcca aguucgccug cuccaagaag augaccggga agagcaucca gccggagaac      960

cuggaguacc gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac     1020

gacaccggcc acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc     1080

cgcgcggagg ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc     1140

ggccuggacu ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc     1320

gugguggucc ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag     1380

gccgagaugg acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg     1560

accgacggcc cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc     1620

guggggcgcc ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag     1740

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

guccgcggcg ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug     1860

ggcggggccc ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg     1980

cucgggcuga acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc     2040

cugaucuucc ucuccaccgc ggugagcgcc gacguggggu gcuccgucga cuucagcaag     2100

aaggagaccc gcugcgggac gggcguguuc guguacaacg acgucgaggc cuggcgggac     2160

cgcuacaagu accaccccga cuccccgcgc cggcuggccg ccgcggugaa gcaggccugg     2220

gaggacggga ucugcggcau cagcuccgug agccgcaugg agaacaucau guggcggagc     2280

gucgaggggg agcugaacgc cauccucgag gagaacggcg ugcagcugac cguggucgug     2340

ggcuccguga agaacccgau guggcgcggg ccccagcggc ugcccguccc ggugaacgag     2400

cucccccacg gcuggaaggc cugggggaag agccacuucg ugcgcgcggc caagaccaac     2460

aacuccuucg ucguggacgg cgacacccug aaggagugcc cccugaagca ccgggccugg     2520

aacagcuucc ucguggagga ccacggguuc ggcgucuucc acacguccgu guggcugaag     2580

gugcgcgagg acuacagccu ggagugcgac cccgccguca ucggcaccgc cgugaagggg     2640

aaggaggcgg ugcacuccga ccucggcuac uggaucgaga gcgagaagaa cgacaccugg     2700

cggcugaagc gcgcccaccu gaucgagaug aagaccugcg aguggccgaa gucccacacg     2760

cucuggaccg acgggaucga ggagagcgac cugaucaucc ccaagucccu ggccggcccc     2820

cucagccacc acaacacccg ggagggguac cgcacccaga ugaagggccc cuggcacucc     2880

gaggagcugg agauccgguu cgaggagugc ccgggcacga agguccacgu ggaggagacc     2940

ugcgggaccc gcggccccag ccugcggucc accacggcca gcgggcgcgu caucgaggag     3000

uggugcugcc gggagugcac caugcccccg cucuccuucc gcgccaagga cggcugcugg     3060

uacggcaugg agauccggcc gcgcaaggag cccgagagca accuggugcg guccauggug     3120

accgcg                                                                3126


<210>  440
<211>  3126
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS-prME-NS1

<400>  440
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag       60

gucacccggc gcggcuccgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc      120

aucagcuucc cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg      180

cacacgugcg acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacg agcaccuggg ucguguacgg cacgugccac      300

cacaagaagg gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacg      360

cgcaagcugc agacccggag ccagaccugg cucgaguccc gcgaguacac caagcaccug      420

auccgggucg agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc      480

gccuggcucc uggggagcuc cacgagccag aaggugaucu accuggugau gauccuccug      540

aucgcccccg cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg      600

auguccggcg gcaccugggu ggacgucgug cuggagcacg ggggcugcgu gaccgucaug      660

gcccaggaca agcccaccgu ggacaucgag cucgugacga ccaccgucag caacauggcc      720

gaggugcgca gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc      780

cccacccagg gggaggcgua ccuggacaag caguccgaca cgcaguacgu cugcaagcgc      840

acccuggugg accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug      900

accugcgcca aguucgccug cuccaagaag augaccggga agagcaucca gccggagaac      960

cuggaguacc gcaucaugcu cuccguccac ggcagccagc acuccgggau gaucgugaac     1020

gacacgggcc acgagaccga cgagaaccgg gccaaggugg agaucacccc caacagcccc     1080

cgcgcggagg ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacg     1140

ggccuggacu ucagcgaccu guacuaccuc accaugaaca acaagcacug gcugguccac     1200

aaggaguggu uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg     1260

cacuggaaca acaaggaggc ccucguggag uucaaggacg cgcacgccaa gcgccagacg     1320

guggucgugc ugggcuccca ggagggggcc gugcacaccg cccuggccgg cgcgcuggag     1380

gccgagaugg acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggggucagc uacucccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgcggagac gcuccacggc accgugaccg uggaggucca guacgccggg     1560

accgacggcc cgugcaaggu gcccgcccag auggccgugg acaugcagac gcugaccccc     1620

gucgggcgcc ugaucaccgc gaaccccgug aucaccgaga gcacggagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac uccuacaucg ugaucggcgu cggggagaag     1740

aagaucaccc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

gugcgcggcg ccaagcggau ggccgugcug ggggacacgg cgugggacuu cggcagcguc     1860

ggcggggccc ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucggcacccu gcugaugugg     1980

cucgggcuga acgccaagaa cggcuccauc agccugaugu gccucgcgcu gggcggcgug     2040

cugaucuucc ucuccaccgc cgugagcgcc gacgucgggu gcuccgugga cuucagcaag     2100

aaggagaccc gcugcgggac gggcguguuc gucuacaacg acguggaggc cuggcgggac     2160

cgcuacaagu accaccccga cuccccgcgc cggcuggccg cggccgugaa gcaggccugg     2220

gaggacggga ucugcggcau cagcuccguc agccgcaugg agaacaucau guggcggagc     2280

guggaggggg agcugaacgc cauccucgag gagaacggcg ugcagcugac cgucguggug     2340

ggcuccguca agaacccgau guggcgcggg ccccagcggc ugcccgugcc ggugaacgag     2400

cucccccacg gcuggaaggc gugggggaag agcuacuucg uccgcgccgc caagaccaac     2460

aacuccuucg ugguggacgg cgacacccug aaggagugcc cccugaagca ccgggccugg     2520

aacagcuucc ucgucgagga ccacggguuc ggcguguucc acacguccgu guggcugaag     2580

guccgcgagg acuacagccu ggagugcgac cccgccguga ucggcaccgc ggugaagggg     2640

aaggaggccg uccacuccga ccucggcuac uggaucgaga gcgagaagaa cgacaccugg     2700

cggcugaagc gcgcccaccu gaucgagaug aagaccugcg aguggccgaa gucccacacg     2760

cucuggaccg acgggaucga ggagagcgac cugaucaucc ccaagucccu ggccggcccc     2820

cucagccacc acaacacccg ggagggguac cgcacccaga ugaagggccc cuggcacucc     2880

gaggagcugg agauccgguu cgaggagugc ccgggcacga aggugcacgu cgaggagacc     2940

ugcgggaccc gcggccccag ccugcggucc accacggcca gcgggcgcgu gaucgaggag     3000

uggugcugcc gggagugcac caugccgccc cucuccuucc gcgcgaagga cggcugcugg     3060

uacggcaugg agauccggcc gcgcaaggag cccgagagca accuggugcg guccaugguc     3120

accgcc                                                                3126


<210>  441
<211>  3114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS-prME-NS1

<400>  441
ggcgccgaca ccagcaucgg gaucaucggc cugcugcuca cgaccgccau ggcggccgag       60

aucacccgcc ggggguccgc cuacuacaug uaccuggacc gcagcgacgc cggcaaggcc      120

aucuccuucg cgaccacgcu gggcgugaac aagugccacg ugcagaucau ggaccucggg      180

cacaugugcg acgccaccau gagcuacgag ugcccgaugc uggacgaggg cgucgagccc      240

gacgacgugg acugcuggug caacaccacc uccacguggg uggucuacgg gaccugccac      300

cacaagaagg gcgaggcccg gcgcagccgg cgcgccguga cccugcccuc ccacagcacc      360

cggaagcucc agacgcgcuc ccagaccugg cuggagagcc gcgaguacac caagcaccug      420

aucaaggugg agaacuggau cuuccggaac cccggcuucg cccucgucgc gguggccauc      480

gccuggcugc ugggguccag caccucccag aaggugaucu accucgucau gauccugcug      540

aucgccccgg ccuacagcau ccgcugcauc ggcgugucca accgggacuu cguggagggg      600

augagcggcg ggacgugggu cgacguggug cucgagcacg gcggcugcgu caccgugaug      660

gcgcaggaca agcccaccgu ggacaucgag cuggucacca cgaccguguc caacauggcc      720

gagguccgca gcuacugcua cgaggccagc aucuccgaca uggccagcga cucccggugc      780

cccacccagg gggaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagcgc      840

acgcucgugg accggggcug ggggaacggc ugcgggcugu ucggcaaggg cucccugguc      900

acgugcgcga aguucacgug cagcaagaag augaccggga aguccaucca gcccgagaac      960

cucgaguacc gcaucaugcu gagcgugcac ggcucccagc acagcgggau gaucggcuac     1020

gagacggacg aggaccgggc caagguggag gucaccccga acuccccgcg cgccgaggcc     1080

acccugggcg gguucggcag ccucgggcug gacugcgagc cccggaccgg ccuggacuuc     1140

uccgaccucu acuaccugac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1200

cacgacaucc cccucccgug gcacgcgggg gccgacaccg gcaccccgca cuggaacaac     1260

aaggaggccc ugguggaguu caaggacgcc cacgccaagc gccagaccgu cguggugcug     1320

ggcagccagg agggggcggu ccacacggcc cuggccggcg cccucgaggc cgagauggac     1380

ggggcgaagg gccggcuguu cuccgggcac cugaagugcc gccucaagau ggacaagcug     1440

cggcugaagg gcgugagcua cucccucugc accgccgccu ucaccuucac caaggugccc     1500

gccgagaccc ugcacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccc     1560

ugcaagaucc cgguccagau ggcgguggac augcagaccc ugacgcccgu ggggcgccuc     1620

aucaccgcca accccgucau caccgagagc accgagaacu ccaagaugau gcuggagcug     1680

gacccgccgu ucggcgacag cuacaucgug aucggggugg gcgacaagaa gaucacgcac     1740

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcg     1800

aagcggaugg ccgugcucgg ggacaccgcc ugggacuucg gcagcguggg cggcgucuuc     1860

aacucccugg ggaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1920

ggcauguccu gguucagcca gauccucauc gggacgcugc ugguguggcu cggccugaac     1980

accaagaacg gcuccaucag ccugaccugc cucgcgcugg gcggcgugau gaucuuccug     2040

uccaccgccg ucagcgccga cguggggugc uccguggacu ucagcaagaa ggagacgcgc     2100

ugcgggaccg gcgucuucau cuacaacgac guggaggccu ggcgcgaccg guacaaguac     2160

caccccgacu ccccgcgccg gcucgccgcg gccgugaagc aggccuggga ggaggggauc     2220

ugcggcauca gcuccgucag ccgcauggag aacaucaugu ggaaguccgu ggagggggag     2280

cugaacgcca uccuggagga gaacggcgug cagcucaccg ucgugguggg gagcgucaag     2340

aaccccaugu ggcggggccc gcagcgccug cccgugcccg ugaacgagcu gccgcacggc     2400

uggaaggccu gggggaagag cuacuucguc cgggcggcca agaccaacaa cuccuucgug     2460

guggacggcg acacgcucaa ggagugcccc cuggagcacc gcgccuggaa cagcuuccug     2520

gucgaggacc acggguucgg cguguuccac accuccgugu ggcucaaggu ccgggaggac     2580

uacagccugg agugcgaccc cgccgugauc gggaccgcgg ucaagggccg cgaggccgcc     2640

cacuccgacc ugggcuacug gaucgagagc gagaagaacg acaccuggcg gcucaagcgc     2700

gcccaccuga ucgagaugaa gacgugcgag uggcccaagu cccacacccu guggaccgac     2760

gggguggagg agagcgaccu caucaucccg aagucccugg ccggcccccu gagccaccac     2820

aacacccggg agggguaccg cacccaggug aagggccccu ggcacuccga ggagcucgag     2880

auccgguucg aggagugccc cgggaccaag gucuacgugg aggagaccug cggcacccgc     2940

ggcccgagcc ugcgguccac gaccgcgagc gggcgcguga ucgaggagug gugcugccgg     3000

gagugcacca ugccgccccu guccuuccgc gccaaggacg gcugcuggua cgggauggag     3060

auccggcccc gcaaggagcc ggagagcaac cucguccgcu ccauggugac cgcc           3114


<210>  442
<211>  3126
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS-prME-NS1

<400>  442
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag       60

gugacccgcc gcggcagcgc cuacuacaug uaccuggacc gcaacgacgc cggcgaggcc      120

aucagcuucc ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcagccgc cgcgccguga cccugcccag ccacagcacc      360

cgcaagcugc agacccgcag ccagaccugg cuggagagcc gcgaguacac caagcaccug      420

auccgcgugg agaacuggau cuuccgcaac cccggcuucg cccuggccgc cgccgccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau ccgcugcauc ggcgugagca accgcgacuu cguggagggc      600

augagcggcg gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugcgca gcuacugcua cgaggccagc aucagcgaca uggccagcga cagccgcugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagcgc      840

acccuggugg accgcggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguacc gcaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaccggcc acgagaccga cgagaaccgc gccaaggugg agaucacccc caacagcccc     1080

cgcgccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gccccgcacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggccgccug agcagcggcc accugaagug ccgccugaag     1440

auggacaagc ugcgccugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggccgcc ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag     1740

aagaucaccc accacuggca ccgcagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgccc ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug     2040

cugaucuucc ugagcaccgc cgugagcgcc gacgugggcu gcagcgugga cuucagcaag     2100

aaggagaccc gcugcggcac cggcguguuc guguacaacg acguggaggc cuggcgcgac     2160

cgcuacaagu accaccccga cagcccccgc cgccuggccg ccgccgugaa gcaggccugg     2220

gaggacggca ucugcggcau cagcagcgug agccgcaugg agaacaucau guggcgcagc     2280

guggagggcg agcugaacgc cauccuggag gagaacggcg ugcagcugac cgugguggug     2340

ggcagcguga agaaccccau guggcgcggc ccccagcgcc ugcccgugcc cgugaacgag     2400

cugccccacg gcuggaaggc cuggggcaag agccacuucg ugcgcgccgc caagaccaac     2460

aacagcuucg ugguggacgg cgacacccug aaggagugcc cccugaagca ccgcgccugg     2520

aacagcuucc ugguggagga ccacggcuuc ggcguguucc acaccagcgu guggcugaag     2580

gugcgcgagg acuacagccu ggagugcgac cccgccguga ucggcaccgc cgugaagggc     2640

aaggaggccg ugcacagcga ccugggcuac uggaucgaga gcgagaagaa cgacaccugg     2700

cgccugaagc gcgcccaccu gaucgagaug aagaccugcg aguggcccaa gagccacacc     2760

cuguggaccg acggcaucga ggagagcgac cugaucaucc ccaagagccu ggccggcccc     2820

cugagccacc acaacacccg cgagggcuac cgcacccaga ugaagggccc cuggcacagc     2880

gaggagcugg agauccgcuu cgaggagugc cccggcacca aggugcacgu ggaggagacc     2940

ugcggcaccc gcggccccag ccugcgcagc accaccgcca gcggccgcgu gaucgaggag     3000

uggugcugcc gcgagugcac caugcccccc cugagcuucc gcgccaagga cggcugcugg     3060

uacggcaugg agauccgccc ccgcaaggag cccgagagca accuggugcg cagcauggug     3120

accgcc                                                                3126


<210>  443
<211>  3126
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS-prME-NS1

<400>  443
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag       60

gugacccgcc gcggcagcgc cuacuacaug uaccuggacc gcaacgacgc cggcgaggcc      120

aucagcuucc ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc      180

cacaccugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcagccgc cgcgccguga cccugcccag ccacagcacc      360

cgcaagcugc agacccgcag ccagaccugg cuggagagcc gcgaguacac caagcaccug      420

auccgcgugg agaacuggau cuuccgcaac cccggcuucg cccuggccgc cgccgccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau ccgcugcauc ggcgugagca accgcgacuu cguggagggc      600

augagcggcg gcaccugggu ggacguggug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugcgca gcuacugcua cgaggccagc aucagcgaca uggccagcga cagccgcugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagcgc      840

acccuggugg accgcggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguacc gcaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaccggcc acgagaccga cgagaaccgc gccaaggugg agaucacccc caacagcccc     1080

cgcgccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gccccgcacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggccgccug agcagcggcc accugaagug ccgccugaag     1440

auggacaagc ugcgccugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggccgcc ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag     1740

aagaucaccc accacuggca ccgcagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgccc ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg     1980

cugggccuga acgccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug     2040

cugaucuucc ugagcaccgc cgugagcgcc gacgugggcu gcagcgugga cuucagcaag     2100

aaggagaccc gcugcggcac cggcguguuc guguacaacg acguggaggc cuggcgcgac     2160

cgcuacaagu accaccccga cagcccccgc cgccuggccg ccgccgugaa gcaggccugg     2220

gaggacggca ucugcggcau cagcagcgug agccgcaugg agaacaucau guggcgcagc     2280

guggagggcg agcugaacgc cauccuggag gagaacggcg ugcagcugac cgugguggug     2340

ggcagcguga agaaccccau guggcgcggc ccccagcgcc ugcccgugcc cgugaacgag     2400

cugccccacg gcuggaaggc cuggggcaag agcuacuucg ugcgcgccgc caagaccaac     2460

aacagcuucg ugguggacgg cgacacccug aaggagugcc cccugaagca ccgcgccugg     2520

aacagcuucc ugguggagga ccacggcuuc ggcguguucc acaccagcgu guggcugaag     2580

gugcgcgagg acuacagccu ggagugcgac cccgccguga ucggcaccgc cgugaagggc     2640

aaggaggccg ugcacagcga ccugggcuac uggaucgaga gcgagaagaa cgacaccugg     2700

cgccugaagc gcgcccaccu gaucgagaug aagaccugcg aguggcccaa gagccacacc     2760

cuguggaccg acggcaucga ggagagcgac cugaucaucc ccaagagccu ggccggcccc     2820

cugagccacc acaacacccg cgagggcuac cgcacccaga ugaagggccc cuggcacagc     2880

gaggagcugg agauccgcuu cgaggagugc cccggcacca aggugcacgu ggaggagacc     2940

ugcggcaccc gcggccccag ccugcgcagc accaccgcca gcggccgcgu gaucgaggag     3000

uggugcugcc gcgagugcac caugcccccc cugagcuucc gcgccaagga cggcugcugg     3060

uacggcaugg agauccgccc ccgcaaggag cccgagagca accuggugcg cagcauggug     3120

accgcc                                                                3126


<210>  444
<211>  3114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS-prME-NS1

<400>  444
ggcgccgaca ccagcaucgg caucaucggc cugcugcuga ccaccgccau ggccgccgag       60

aucacccgcc gcggcagcgc cuacuacaug uaccuggacc gcagcgacgc cggcaaggcc      120

aucagcuucg ccaccacccu gggcgugaac aagugccacg ugcagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcagccgc cgcgccguga cccugcccag ccacagcacc      360

cgcaagcugc agacccgcag ccagaccugg cuggagagcc gcgaguacac caagcaccug      420

aucaaggugg agaacuggau cuuccgcaac cccggcuucg cccugguggc cguggccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau ccgcugcauc ggcgugagca accgcgacuu cguggagggc      600

augagcggcg gcaccugggu ggacguggug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugcgca gcuacugcua cgaggccagc aucagcgaca uggccagcga cagccgcugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagcgc      840

acccuggugg accgcggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucaccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguacc gcaucaugcu gagcgugcac ggcagccagc acagcggcau gaucggcuac     1020

gagaccgacg aggaccgcgc caagguggag gugaccccca acagcccccg cgccgaggcc     1080

acccugggcg gcuucggcag ccugggccug gacugcgagc cccgcaccgg ccuggacuuc     1140

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1200

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1260

aaggaggccc ugguggaguu caaggacgcc cacgccaagc gccagaccgu gguggugcug     1320

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1380

ggcgccaagg gccgccuguu cagcggccac cugaagugcc gccugaagau ggacaagcug     1440

cgccugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caaggugccc     1500

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1560

ugcaagaucc ccgugcagau ggccguggac augcagaccc ugacccccgu gggccgccug     1620

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1680

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgacaagaa gaucacccac     1740

cacuggcacc gcagcggcag caccaucggc aaggccuucg aggccaccgu gcgcggcgcc     1800

aagcgcaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcguguuc     1860

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1920

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugguguggcu gggccugaac     1980

accaagaacg gcagcaucag ccugaccugc cuggcccugg gcggcgugau gaucuuccug     2040

agcaccgccg ugagcgccga cgugggcugc agcguggacu ucagcaagaa ggagacccgc     2100

ugcggcaccg gcguguucau cuacaacgac guggaggccu ggcgcgaccg cuacaaguac     2160

caccccgaca gcccccgccg ccuggccgcc gccgugaagc aggccuggga ggagggcauc     2220

ugcggcauca gcagcgugag ccgcauggag aacaucaugu ggaagagcgu ggagggcgag     2280

cugaacgcca uccuggagga gaacggcgug cagcugaccg uggugguggg cagcgugaag     2340

aaccccaugu ggcgcggccc ccagcgccug cccgugcccg ugaacgagcu gccccacggc     2400

uggaaggccu ggggcaagag cuacuucgug cgcgccgcca agaccaacaa cagcuucgug     2460

guggacggcg acacccugaa ggagugcccc cuggagcacc gcgccuggaa cagcuuccug     2520

guggaggacc acggcuucgg cguguuccac accagcgugu ggcugaaggu gcgcgaggac     2580

uacagccugg agugcgaccc cgccgugauc ggcaccgccg ugaagggccg cgaggccgcc     2640

cacagcgacc ugggcuacug gaucgagagc gagaagaacg acaccuggcg ccugaagcgc     2700

gcccaccuga ucgagaugaa gaccugcgag uggcccaaga gccacacccu guggaccgac     2760

ggcguggagg agagcgaccu gaucaucccc aagagccugg ccggcccccu gagccaccac     2820

aacacccgcg agggcuaccg cacccaggug aagggccccu ggcacagcga ggagcuggag     2880

auccgcuucg aggagugccc cggcaccaag guguacgugg aggagaccug cggcacccgc     2940

ggccccagcc ugcgcagcac caccgccagc ggccgcguga ucgaggagug gugcugccgc     3000

gagugcacca ugcccccccu gagcuuccgc gccaaggacg gcugcuggua cggcauggag     3060

auccgccccc gcaaggagcc cgagagcaac cuggugcgca gcauggugac cgcc           3114


<210>  445
<211>  544
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 10aa-SS-E-10aa

<400>  445

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ile Arg 
            20                  25                  30          


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        35                  40                  45              


Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    50                  55                  60                  


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
65                  70                  75                  80  


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                85                  90                  95      


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            100                 105                 110         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        115                 120                 125             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    130                 135                 140                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
145                 150                 155                 160 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                165                 170                 175     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            180                 185                 190         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        195                 200                 205             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    210                 215                 220                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
225                 230                 235                 240 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                245                 250                 255     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            260                 265                 270         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        275                 280                 285             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    290                 295                 300                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
305                 310                 315                 320 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                325                 330                 335     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            340                 345                 350         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        355                 360                 365             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    370                 375                 380                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
385                 390                 395                 400 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                405                 410                 415     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            420                 425                 430         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        435                 440                 445             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    450                 455                 460                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
465                 470                 475                 480 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                485                 490                 495     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly 
            500                 505                 510         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        515                 520                 525             


Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    530                 535                 540                 


<210>  446
<211>  544
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 10aa-SS-E-10aa

<400>  446

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ile Arg 
            20                  25                  30          


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        35                  40                  45              


Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    50                  55                  60                  


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
65                  70                  75                  80  


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                85                  90                  95      


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            100                 105                 110         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        115                 120                 125             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    130                 135                 140                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
145                 150                 155                 160 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                165                 170                 175     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            180                 185                 190         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        195                 200                 205             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    210                 215                 220                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
225                 230                 235                 240 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                245                 250                 255     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            260                 265                 270         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        275                 280                 285             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    290                 295                 300                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
305                 310                 315                 320 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                325                 330                 335     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            340                 345                 350         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        355                 360                 365             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    370                 375                 380                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
385                 390                 395                 400 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                405                 410                 415     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            420                 425                 430         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        435                 440                 445             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    450                 455                 460                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
465                 470                 475                 480 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                485                 490                 495     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys Asn Gly 
            500                 505                 510         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        515                 520                 525             


Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    530                 535                 540                 


<210>  447
<211>  540
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) 10aa-SS-E-10aa

<400>  447

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ile Arg 
            20                  25                  30          


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        35                  40                  45              


Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    50                  55                  60                  


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
65                  70                  75                  80  


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                85                  90                  95      


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            100                 105                 110         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        115                 120                 125             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    130                 135                 140                 


Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
145                 150                 155                 160 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                165                 170                 175     


Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys 
            180                 185                 190         


Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        195                 200                 205             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    210                 215                 220                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
225                 230                 235                 240 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                245                 250                 255     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            260                 265                 270         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        275                 280                 285             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    290                 295                 300                 


Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys 
305                 310                 315                 320 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                325                 330                 335     


Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val 
            340                 345                 350         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro 
        355                 360                 365             


Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    370                 375                 380                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
385                 390                 395                 400 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                405                 410                 415     


Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            420                 425                 430         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        435                 440                 445             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe 
    450                 455                 460                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
465                 470                 475                 480 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                485                 490                 495     


Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            500                 505                 510         


Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val 
        515                 520                 525             


Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    530                 535                 540 


<210>  448
<211>  1632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 10aa-SS-E-10aa

<400>  448
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca aucaggugca uaggagucag caauagggac      120

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      180

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      240

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      300

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      360

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      420

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      480

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg      540

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg      600

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu      660

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac      720

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac      780

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc      840

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu      900

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa      960

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1020

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1080

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1140

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1200

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1260

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1320

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1380

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1440

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1500

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     1560

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     1620

gacuucucaa ag                                                         1632


<210>  449
<211>  1632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 10aa-SS-E-10aa

<400>  449
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca aucaggugca uaggagucag caauagggac      120

uuuguggaag guaugucagg ugggacuugg guugauguug ucuuggaaca uggagguugu      180

gucacuguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      240

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      300

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      360

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      420

gggagccugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      480

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg      540

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaagu ugagauaacg      600

cccaauucac caagagccga agccacccug gggggguuug gaagccuagg acuugauugu      660

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac      720

uggcugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac      780

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc      840

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu      900

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa      960

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguacugca     1020

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1080

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1140

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1200

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1260

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1320

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1380

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aaucuuugga     1440

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1500

uugcugaugu gguugggucu gaacgcaaag aauggaucua uuucccuuau gugcuuggcc     1560

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     1620

gacuucucaa ag                                                         1632


<210>  450
<211>  1620
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) 10aa-SS-E-10aa

<400>  450
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca aucaggugca uuggagucag caauagagac      120

uucguggagg gcaugucagg ugggaccugg guugauguug ucuuggaaca uggaggcugc      180

guuaccguga uggcacagga caagccaaca gucgacauag aguuggucac gacgacgguu      240

aguaacaugg ccgagguaag auccuauugc uacgaggcau cgauaucgga cauggcuucg      300

gacagucguu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      360

gucugcaaaa gaacauuagu ggacagaggu uggggaaacg guuguggacu uuuuggcaaa      420

gggagcuugg ugacaugugc caaguuuacg uguucuaaga agaugaccgg gaagagcauu      480

caaccggaaa aucuggagua ucggauaaug cuaucagugc auggcuccca gcauagcggg      540

augauuggau augaaacuga cgaagauaga gcgaaagucg agguuacgcc uaauucacca      600

agagcggaag caaccuuggg aggcuuugga agcuuaggac uugacuguga accaaggaca      660

ggccuugacu uuucagaucu guauuaccug accaugaaca auaagcauug guuggugcac      720

aaagaguggu uucaugacau cccauugccu uggcaugcug gggcagacac cggaacucca      780

cacuggaaca acaaagaggc auugguagaa uucaaggaug cccacgccaa gaggcaaacc      840

gucgucguuc uggggagcca ggaaggagcc guucacacgg cucucgcugg agcucuagag      900

gcugagaugg auggugcaaa gggaaggcug uucucuggcc auuugaaaug ccgccuaaaa      960

auggacaagc uuagauugaa gggcguguca uauuccuugu gcacugcggc auucacauuc     1020

accaaggucc cagcugaaac acugcaugga acagucacag uggaggugca guaugcaggg     1080

acagauggac ccugcaagau cccaguccag auggcggugg acaugcagac ccugacccca     1140

guuggaaggc ugauaaccgc caaccccgug auuacugaaa gcacugagaa cucaaagaug     1200

auguuggagc uugacccacc auuuggggau ucuuacauug ucauaggagu uggggacaag     1260

aaaaucaccc accacuggca uaggaguggu agcaccaucg gaaaggcauu ugaggccacu     1320

gugagaggcg ccaagagaau ggcaguccug ggggauacag ccugggacuu cggaucaguc     1380

gggggugugu ucaacucacu ggguaagggc auucaccaga uuuuuggagc agccuucaaa     1440

ucacuguuug gaggaauguc cugguucuca cagauccuca uaggcacgcu gcuagugugg     1500

uuagguuuga acacaaagaa uggaucuauc ucccucacau gcuuggcccu ggggggagug     1560

augaucuucc ucuccacggc uguuucugcu gacguggggu gcucagugga cuucucaaaa     1620


<210>  451
<211>  1632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 10aa-SS-E-10aa

<400>  451
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg auccgcugca ucggcgucag caaccgggac      120

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      180

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      240

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      300

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      360

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      420

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc      480

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg      540

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc      600

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc      660

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac      720

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac      780

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc      840

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc      900

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag      960

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1020

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1080

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1140

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1200

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1260

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1320

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1380

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1440

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1500

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     1560

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcuccguc     1620

gacuucagca ag                                                         1632


<210>  452
<211>  1632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 10aa-SS-E-10aa

<400>  452
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg auccgcugca ucggcgucag caaccgggac      120

uucguggagg ggauguccgg cggcaccugg guggacgucg ugcuggagca cgggggcugc      180

gugaccguca uggcccagga caagcccacc guggacaucg agcucgugac gaccaccguc      240

agcaacaugg ccgaggugcg cagcuacugc uacgaggccu ccaucagcga cauggccucc      300

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacgcaguac      360

gucugcaagc gcacccuggu ggaccggggc ugggggaacg gcugcggccu cuucgggaag      420

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc      480

cagccggaga accuggagua ccgcaucaug cucuccgucc acggcagcca gcacuccggg      540

augaucguga acgacacggg ccacgagacc gacgagaacc gggccaaggu ggagaucacc      600

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc      660

gagccccgga cgggccugga cuucagcgac cuguacuacc ucaccaugaa caacaagcac      720

uggcuggucc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac      780

accgggaccc cgcacuggaa caacaaggag gcccucgugg aguucaagga cgcgcacgcc      840

aagcgccaga cgguggucgu gcugggcucc caggaggggg ccgugcacac cgcccuggcc      900

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag      960

ugccgccuga agauggacaa gcuccggcug aaggggguca gcuacucccu gugcaccgcc     1020

gccuucaccu ucaccaagau ccccgcggag acgcuccacg gcaccgugac cguggagguc     1080

caguacgccg ggaccgacgg cccgugcaag gugcccgccc agauggccgu ggacaugcag     1140

acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg ugaucaccga gagcacggag     1200

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1260

gucggggaga agaagaucac ccaccacugg caccggagcg gcuccaccau cgggaaggcc     1320

uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc ugggggacac ggcgugggac     1380

uucggcagcg ucggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1440

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacc     1500

cugcugaugu ggcucgggcu gaacgccaag aacggcucca ucagccugau gugccucgcg     1560

cugggcggcg ugcugaucuu ccucuccacc gccgugagcg ccgacgucgg gugcuccgug     1620

gacuucagca ag                                                         1632


<210>  453
<211>  1620
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) 10aa-SS-E-10aa

<400>  453
cggaucauca acgcccgcaa ggagcggaag cgccggggcg ccgacaccag caucgggauc       60

aucggccugc ugcucacgac cgccauggcg auccgcugca ucggcguguc caaccgggac      120

uucguggagg ggaugagcgg cgggacgugg gucgacgugg ugcucgagca cggcggcugc      180

gucaccguga uggcgcagga caagcccacc guggacaucg agcuggucac cacgaccgug      240

uccaacaugg ccgagguccg cagcuacugc uacgaggcca gcaucuccga cauggccagc      300

gacucccggu gccccaccca gggggaggcc uaccuggaca agcagagcga cacccaguac      360

gugugcaagc gcacgcucgu ggaccggggc ugggggaacg gcugcgggcu guucggcaag      420

ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga agaugaccgg gaaguccauc      480

cagcccgaga accucgagua ccgcaucaug cugagcgugc acggcuccca gcacagcggg      540

augaucggcu acgagacgga cgaggaccgg gccaaggugg aggucacccc gaacuccccg      600

cgcgccgagg ccacccuggg cggguucggc agccucgggc uggacugcga gccccggacc      660

ggccuggacu ucuccgaccu cuacuaccug acgaugaaca acaagcacug gcuggugcac      720

aaggaguggu uccacgacau cccccucccg uggcacgcgg gggccgacac cggcaccccg      780

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc      840

gucguggugc ugggcagcca ggagggggcg guccacacgg cccuggccgg cgcccucgag      900

gccgagaugg acggggcgaa gggccggcug uucuccgggc accugaagug ccgccucaag      960

auggacaagc ugcggcugaa gggcgugagc uacucccucu gcaccgccgc cuucaccuuc     1020

accaaggugc ccgccgagac ccugcacggc acggucaccg uggaggugca guacgccggg     1080

accgacggcc ccugcaagau cccgguccag auggcggugg acaugcagac ccugacgccc     1140

guggggcgcc ucaucaccgc caaccccguc aucaccgaga gcaccgagaa cuccaagaug     1200

augcuggagc uggacccgcc guucggcgac agcuacaucg ugaucggggu gggcgacaag     1260

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1320

guccgcggcg cgaagcggau ggccgugcuc ggggacaccg ccugggacuu cggcagcgug     1380

ggcggcgucu ucaacucccu ggggaagggg auccaccaga ucuucggcgc cgccuucaag     1440

agccuguucg ggggcauguc cugguucagc cagauccuca ucgggacgcu gcuggugugg     1500

cucggccuga acaccaagaa cggcuccauc agccugaccu gccucgcgcu gggcggcgug     1560

augaucuucc uguccaccgc cgucagcgcc gacguggggu gcuccgugga cuucagcaag     1620


<210>  454
<211>  1632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 10aa-SS-E-10aa

<400>  454
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc auccgcugca ucggcgugag caaccgcgac      120

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      180

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      240

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc      300

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      360

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag      420

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      480

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc      540

augaucguga acgacaccgg ccacgagacc gacgagaacc gcgccaaggu ggagaucacc      600

cccaacagcc cccgcgccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc      660

gagccccgca ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac      720

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac      780

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc      840

aagcgccaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc      900

ggcgcccugg aggccgagau ggacggcgcc aagggccgcc ugagcagcgg ccaccugaag      960

ugccgccuga agauggacaa gcugcgccug aagggcguga gcuacagccu gugcaccgcc     1020

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1080

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1140

acccugaccc ccgugggccg ccugaucacc gccaaccccg ugaucaccga gagcaccgag     1200

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1260

gugggcgaga agaagaucac ccaccacugg caccgcagcg gcagcaccau cggcaaggcc     1320

uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc ugggcgacac cgccugggac     1380

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1440

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1500

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     1560

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     1620

gacuucagca ag                                                         1632


<210>  455
<211>  1632
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 10aa-SS-E-10aa

<400>  455
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc auccgcugca ucggcgugag caaccgcgac      120

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      180

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      240

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc      300

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      360

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag      420

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      480

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc      540

augaucguga acgacaccgg ccacgagacc gacgagaacc gcgccaaggu ggagaucacc      600

cccaacagcc cccgcgccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc      660

gagccccgca ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac      720

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac      780

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc      840

aagcgccaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc      900

ggcgcccugg aggccgagau ggacggcgcc aagggccgcc ugagcagcgg ccaccugaag      960

ugccgccuga agauggacaa gcugcgccug aagggcguga gcuacagccu gugcaccgcc     1020

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1080

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1140

acccugaccc ccgugggccg ccugaucacc gccaaccccg ugaucaccga gagcaccgag     1200

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1260

gugggcgaga agaagaucac ccaccacugg caccgcagcg gcagcaccau cggcaaggcc     1320

uucgaggcca ccgugcgcgg cgccaagcgc auggccgugc ugggcgacac cgccugggac     1380

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1440

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1500

cugcugaugu ggcugggccu gaacgccaag aacggcagca ucagccugau gugccuggcc     1560

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     1620

gacuucagca ag                                                         1632


<210>  456
<211>  1620
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) 10aa-SS-E-10aa

<400>  456
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc auccgcugca ucggcgugag caaccgcgac      120

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      180

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      240

agcaacaugg ccgaggugcg cagcuacugc uacgaggcca gcaucagcga cauggccagc      300

gacagccgcu gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      360

gugugcaagc gcacccuggu ggaccgcggc uggggcaacg gcugcggccu guucggcaag      420

ggcagccugg ugaccugcgc caaguucacc ugcagcaaga agaugaccgg caagagcauc      480

cagcccgaga accuggagua ccgcaucaug cugagcgugc acggcagcca gcacagcggc      540

augaucggcu acgagaccga cgaggaccgc gccaaggugg aggugacccc caacagcccc      600

cgcgccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gccccgcacc      660

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac      720

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc      780

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc      840

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag      900

gccgagaugg acggcgccaa gggccgccug uucagcggcc accugaagug ccgccugaag      960

auggacaagc ugcgccugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1020

accaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1080

accgacggcc ccugcaagau ccccgugcag auggccgugg acaugcagac ccugaccccc     1140

gugggccgcc ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1200

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     1260

aagaucaccc accacuggca ccgcagcggc agcaccaucg gcaaggccuu cgaggccacc     1320

gugcgcggcg ccaagcgcau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1380

ggcggcgugu ucaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1440

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcuggugugg     1500

cugggccuga acaccaagaa cggcagcauc agccugaccu gccuggcccu gggcggcgug     1560

augaucuucc ugagcaccgc cgugagcgcc gacgugggcu gcagcgugga cuucagcaag     1620


<210>  457
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human ribosomal protein Large 32 lacking the 5 terminal 
       oligopyrimidine tract

<400>  457
ggcgctgcct acggaggtgg cagccatctc cttctcggca tc                          42


<210>  458
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human ribosomal protein Large 32 lacking the 5 terminal 
       oligopyrimidine tract mRNA

<400>  458
ggcgcugccu acggaggugg cagccaucuc cuucucggca uc                          42


<210>  459
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human mitochondrial ATP synthase subunit alpha lacking 
       the 5 terminal oligopyrimidine tract

<400>  459
gcggctcggc cattttgtcc cagtcagtcc ggaggctgcg gctgcagaag taccgcctgc       60

ggagtaactg caaag                                                        75


<210>  460
<211>  75
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human mitochondrial ATP synthase subunit alpha lacking 
       the 5 terminal oligopyrimidine tract mRNA

<400>  460
gcggcucggc cauuuugucc cagucagucc ggaggcugcg gcugcagaag uaccgccugc       60

ggaguaacug caaag                                                        75


<210>  461
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human ribosomal protein Large 32 lacking the 5 terminal 
       oligopyrimidine tract

<400>  461
gagaggcgct gcctacggag gtggcagcca tctccttctc ggcatc                      46


<210>  462
<211>  46
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human ribosomal protein Large 32 lacking the 5 terminal 
       oligopyrimidine tract mRNA

<400>  462
gagaggcgcu gccuacggag guggcagcca ucuccuucuc ggcauc                      46


<210>  463
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human peroxisomal multifunctional enzyme type 2 lacking 
       the 5 terminal oligopyrimidine tract

<400>  463
gtcccgcagt cggcgtccag cggctctgct tgttcgtgtg tgtgtcgttg caggccttat       60

tc                                                                      62


<210>  464
<211>  62
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human peroxisomal multifunctional enzyme type 2 lacking 
       the 5 terminal oligopyrimidine tract mRNA

<400>  464
gucccgcagu cggcguccag cggcucugcu uguucgugug ugugucguug caggccuuau       60

uc                                                                      62


<210>  465
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human androgen-induced gene 1 protein lacking the 5 
       terminal oligopyrimidine tract

<400>  465
gccgcccagc cggtccaggc ctctggcgaa c                                      31


<210>  466
<211>  31
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human androgen-induced gene 1 protein lacking the 5 
       terminal oligopyrimidine tract mRNA

<400>  466
gccgcccagc cgguccaggc cucuggcgaa c                                      31


<210>  467
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human acid ceramidase lacking the 5 terminal 
       oligopyrimidine tract

<400>  467
gcctctgctg gagtccgggg agtggcgttg gctgctagag cg                          42


<210>  468
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human acid ceramidase lacking the 5 terminal 
       oligopyrimidine tract mRNA

<400>  468
gccucugcug gaguccgggg aguggcguug gcugcuagag cg                          42


<210>  469
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human cytochrome c oxidase subunit 6C lacking the 5 
       terminal oligopyrimidine tract

<400>  469
agtcaggaag gacgttggtg ttgaggttag catacgtatc aaggacagta actacc           56


<210>  470
<211>  56
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human cytochrome c oxidase subunit 6C lacking the 5 
       terminal oligopyrimidine tract mRNA

<400>  470
agucaggaag gacguuggug uugagguuag cauacguauc aaggacagua acuacc           56


<210>  471
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human 60S ribosomal protein L35a lacking the 5 terminal 
       oligopyrimidine tract

<400>  471
accgccatct tggctcctgt ggaggcctgc tgggaacggg acttctaaaa ggaact           56


<210>  472
<211>  56
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human 60S ribosomal protein L35a lacking the 5 terminal 
       oligopyrimidine tract mRNA

<400>  472
accgccaucu uggcuccugu ggaggccugc ugggaacggg acuucuaaaa ggaacu           56


<210>  473
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human ATP binding cassette subfamily B member 7 lacking 
       the 5 terminal oligopyrimidine tract

<400>  473
ggttcctctt tcctcgctca ag                                                22


<210>  474
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-UTR of human ATP binding cassette subfamily B member 7 lacking 
       the 5 terminal oligopyrimidine tract mRNA

<400>  474
gguuccucuu uccucgcuca ag                                                22


<210>  475
<211>  186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3-UTR of human albumin

<400>  475
catcacattt aaaagcatct cagcctacca tgagaataag agaaagaaaa tgaagatcaa       60

aagcttattc atctgttttt ctttttcgtt ggtgtaaagc caacaccctg tctaaaaaac      120

ataaatttct ttaatcattt tgcctctttt ctctgtgctt caattaataa aaaatggaaa      180

gaatct                                                                 186


<210>  476
<211>  186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3-UTR of human albumin mRNA

<400>  476
caucacauuu aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa       60

aagcuuauuc aucuguuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac      120

auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa      180

gaaucu                                                                 186


<210>  477
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3-UTR of Homo sapiens hemoglobin, alpha 1 (HBA1)

<400>  477
gctggagcct cggtggccat gcttcttgcc ccttgggcct ccccccagcc cctcctcccc       60

ttcctgcacc cgtacccccg tggtctttga ataaagtctg agtgggcggc                 110


<210>  478
<211>  110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3-UTR of Homo sapiens hemoglobin, alpha 1 (HBA1) mRNA

<400>  478
gcuggagccu cgguggccau gcuucuugcc ccuugggccu ccccccagcc ccuccucccc       60

uuccugcacc cguacccccg uggucuuuga auaaagucug agugggcggc                 110


<210>  479
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3-UTR of Homo sapiens hemoglobin, alpha 2 (HBA2)

<400>  479
gctggagcct cggtagccgt tcctcctgcc cgctgggcct cccaacgggc cctcctcccc       60

tccttgcacc ggcccttcct ggtctttgaa taaagtctga gtgggcag                   108


<210>  480
<211>  108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3-UTR of Homo sapiens hemoglobin, alpha 2 (HBA2) mRNA

<400>  480
gcuggagccu cgguagccgu uccuccugcc cgcugggccu cccaacgggc ccuccucccc       60

uccuugcacc ggcccuuccu ggucuuugaa uaaagucuga gugggcag                   108


<210>  481
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3-UTR of Homo sapiens hemoglobin, beta (HBB)

<400>  481
gctcgctttc ttgctgtcca atttctatta aaggttcctt tgttccctaa gtccaactac       60

taaactgggg gatattatga agggccttga gcatctggat tctgcctaat aaaaaacatt      120

tattttcatt gc                                                          132


<210>  482
<211>  132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3-UTR of Homo sapiens hemoglobin, beta (HBB) mRNA

<400>  482
gcucgcuuuc uugcugucca auuucuauua aagguuccuu uguucccuaa guccaacuac       60

uaaacugggg gauauuauga agggccuuga gcaucuggau ucugccuaau aaaaaacauu      120

uauuuucauu gc                                                          132


<210>  483
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Center, alpha-complex-binding portion of the 3UTR of an 
       alpha-globin gene (muag)

<400>  483
gcccgatggg cctcccaacg ggccctcctc ccctccttgc accg                        44


<210>  484
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Center, alpha-complex-binding portion of the 3UTR of an 
       alpha-globin gene (muag) mRNA

<400>  484
gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accg                        44


<210>  485
<211>  186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  albumin7 3UTR

<400>  485
catcacattt aaaagcatct cagcctacca tgagaataag agaaagaaaa tgaagatcaa       60

tagcttattc atctcttttt ctttttcgtt ggtgtaaagc caacaccctg tctaaaaaac      120

ataaatttct ttaatcattt tgcctctttt ctctgtgctt caattaataa aaaatggaaa      180

gaacct                                                                 186


<210>  486
<211>  186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  albumin7 3UTR mRNA

<400>  486
caucacauuu aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa       60

uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac      120

auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa      180

gaaccu                                                                 186


<210>  487
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  histone stem-loop sequence

<400>  487
caaaggctct tttcagagcc acca                                              24


<210>  488
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  histone stem-loop sequence mRNA

<400>  488
caaaggcucu uuucagagcc acca                                              24


<210>  489
<211>  1918
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R1548, R2429, Flu A HA

<400>  489
gggagaaagc uuaccaugaa ggccauccug gugguccucc uguacaccuu cgccaccgcg       60

aacgccgaca cgcugugcau cggcuaccac gccaacaaca gcaccgacac cguggacacc      120

gugcucgaga agaacgucac ggugacccac uccgugaacc ugcuggagga caagcacaac      180

gggaagcucu gcaagcugcg gggcgucgcc ccgcugcacc ucgggaagug caacaucgcc      240

ggcuggaucc uggggaaccc ggagugcgag agccugucca ccgcgagcuc cuggagcuac      300

aucguggaga ccuccagcuc cgacaacggc acgugcuacc ccggcgacuu caucgacuac      360

gaggagcucc gcgagcagcu gagcuccgug agcuccuucg agcgguucga gaucuucccc      420

aagaccagcu ccuggcccaa ccacgacagc aacaaggggg ucaccgccgc cugcccgcac      480

gccggcgcga aguccuucua caagaaccug aucuggcucg ugaagaaggg gaacagcuac      540

cccaagcugu ccaagagcua caucaacgac aagggcaagg aggugcuggu ccucuggggg      600

auccaccacc ccagcaccuc cgccgaccag cagagccugu accagaacgc cgacgccuac      660

guguucgugg gcuccagccg cuacuccaag aaguucaagc ccgagaucgc cauccggccg      720

aagguccgcg accaggaggg ccggaugaac uacuacugga cgcuggugga gcccggggac      780

aagaucaccu ucgaggcgac cggcaaccuc gugguccccc gcuacgccuu cgccauggag      840

cggaacgccg ggagcggcau caucaucucc gacacccccg ugcacgacug caacacgacc      900

ugccagaccc cgaagggcgc caucaacacc agccugcccu uccagaacau ccaccccauc      960

acgaucggga agugccccaa guacgugaag uccaccaagc ugcgccucgc gaccggccug     1020

cggaacgucc cgagcaucca gucccgcggg cuguucggcg ccaucgccgg guucaucgag     1080

ggcggcugga ccgggauggu ggacggcugg uacggguacc accaccagaa cgagcagggc     1140

agcggguacg ccgccgaccu caaguccacg cagaacgcga ucgacgagau caccaacaag     1200

gugaacagcg ucaucgagaa gaugaacacc caguucaccg ccgugggcaa ggaguucaac     1260

caccuggaga agcggaucga gaaccugaac aagaaggucg acgacggcuu ccucgacauc     1320

uggacguaca acgccgagcu gcuggugcuc cuggagaacg agcgcacccu ggacuaccac     1380

gacuccaacg ugaagaaccu cuacgagaag guccggagcc agcugaagaa caacgccaag     1440

gagaucggga acggcugcuu cgaguucuac cacaagugcg acaacaccug cauggagucc     1500

gugaagaacg ggaccuacga cuaccccaag uacagcgagg aggccaagcu gaaccgcgag     1560

gagaucgacg gcgugaagcu cgaguccacg cggaucuacc agauccuggc gaucuacagc     1620

accgucgcca gcucccuggu gcucgugguc agccuggggg ccaucuccuu cuggaugugc     1680

agcaacggcu cccugcagug ccgcaucugc aucugaccac uaguuauaag acugacuagc     1740

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1860

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1918


<210>  490
<211>  1870
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R1265, R2569, Luciferase

<400>  490
gggagaaagc uugaggaugg aggacgccaa gaacaucaag aagggcccgg cgcccuucua       60

cccgcuggag gacgggaccg ccggcgagca gcuccacaag gccaugaagc gguacgcccu      120

ggugccgggc acgaucgccu ucaccgacgc ccacaucgag gucgacauca ccuacgcgga      180

guacuucgag augagcgugc gccuggccga ggccaugaag cgguacggcc ugaacaccaa      240

ccaccggauc guggugugcu cggagaacag ccugcaguuc uucaugccgg ugcugggcgc      300

ccucuucauc ggcguggccg ucgccccggc gaacgacauc uacaacgagc gggagcugcu      360

gaacagcaug gggaucagcc agccgaccgu gguguucgug agcaagaagg gccugcagaa      420

gauccugaac gugcagaaga agcugcccau cauccagaag aucaucauca uggacagcaa      480

gaccgacuac cagggcuucc agucgaugua cacguucgug accagccacc ucccgccggg      540

cuucaacgag uacgacuucg ucccggagag cuucgaccgg gacaagacca ucgcccugau      600

caugaacagc agcggcagca ccggccugcc gaagggggug gcccugccgc accggaccgc      660

cugcgugcgc uucucgcacg cccgggaccc caucuucggc aaccagauca ucccggacac      720

cgccauccug agcguggugc cguuccacca cggcuucggc auguucacga cccugggcua      780

ccucaucugc ggcuuccggg ugguccugau guaccgguuc gaggaggagc uguuccugcg      840

gagccugcag gacuacaaga uccagagcgc gcugcucgug ccgacccugu ucagcuucuu      900

cgccaagagc acccugaucg acaaguacga ccugucgaac cugcacgaga ucgccagcgg      960

gggcgccccg cugagcaagg aggugggcga ggccguggcc aagcgguucc accucccggg     1020

cauccgccag ggcuacggcc ugaccgagac cacgagcgcg auccugauca cccccgaggg     1080

ggacgacaag ccgggcgccg ugggcaaggu ggucccguuc uucgaggcca agguggugga     1140

ccuggacacc ggcaagaccc ugggcgugaa ccagcggggc gagcugugcg ugcgggggcc     1200

gaugaucaug agcggcuacg ugaacaaccc ggaggccacc aacgcccuca ucgacaagga     1260

cggcuggcug cacagcggcg acaucgccua cugggacgag gacgagcacu ucuucaucgu     1320

cgaccggcug aagucgcuga ucaaguacaa gggcuaccag guggcgccgg ccgagcugga     1380

gagcauccug cuccagcacc ccaacaucuu cgacgccggc guggccgggc ugccggacga     1440

cgacgccggc gagcugccgg ccgcgguggu ggugcuggag cacggcaaga ccaugacgga     1500

gaaggagauc gucgacuacg uggccagcca ggugaccacc gccaagaagc ugcggggcgg     1560

cgugguguuc guggacgagg ucccgaaggg ccugaccggg aagcucgacg cccggaagau     1620

ccgcgagauc cugaucaagg ccaagaaggg cggcaagauc gccguguaag acuaguuaua     1680

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     1740

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1860

caccagaauu                                                            1870


<210>  491
<211>  713
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804)

<400>  491

Met Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp 
1               5                   10                  15      


Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
            20                  25                  30          


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
        35                  40                  45              


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
    50                  55                  60                  


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
65                  70                  75                  80  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
                85                  90                  95      


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
            100                 105                 110         


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
        115                 120                 125             


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
    130                 135                 140                 


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
145                 150                 155                 160 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
                165                 170                 175     


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
            180                 185                 190         


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
        195                 200                 205             


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
    210                 215                 220                 


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
225                 230                 235                 240 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
                245                 250                 255     


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
            260                 265                 270         


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
        275                 280                 285             


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
    290                 295                 300                 


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
305                 310                 315                 320 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
                325                 330                 335     


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
            340                 345                 350         


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
        355                 360                 365             


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
    370                 375                 380                 


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
385                 390                 395                 400 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
                405                 410                 415     


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
            420                 425                 430         


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
        435                 440                 445             


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
    450                 455                 460                 


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
465                 470                 475                 480 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
                485                 490                 495     


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
            500                 505                 510         


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
        515                 520                 525             


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
    530                 535                 540                 


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
545                 550                 555                 560 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
                565                 570                 575     


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
            580                 585                 590         


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
        595                 600                 605             


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
    610                 615                 620                 


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
625                 630                 635                 640 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
                645                 650                 655     


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
            660                 665                 670         


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
        675                 680                 685             


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 700                 


Val Gly Cys Ser Val Asp Phe Ser Lys 
705                 710             


<210>  492
<211>  452
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723)

<400>  492

Met Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
1               5                   10                  15      


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
            20                  25                  30          


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
        35                  40                  45              


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
    50                  55                  60                  


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
65                  70                  75                  80  


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
                85                  90                  95      


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
            100                 105                 110         


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
        115                 120                 125             


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
    130                 135                 140                 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
145                 150                 155                 160 


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
                165                 170                 175     


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
            180                 185                 190         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        195                 200                 205             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    210                 215                 220                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
225                 230                 235                 240 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                245                 250                 255     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            260                 265                 270         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        275                 280                 285             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
    290                 295                 300                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
305                 310                 315                 320 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
                325                 330                 335     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            340                 345                 350         


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
        355                 360                 365             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    370                 375                 380                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
385                 390                 395                 400 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
                405                 410                 415     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            420                 425                 430         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        435                 440                 445             


Asp Phe Gly Ser 
    450         


<210>  493
<211>  713
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804)

<400>  493

Met Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp 
1               5                   10                  15      


Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
            20                  25                  30          


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
        35                  40                  45              


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
    50                  55                  60                  


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met 
65                  70                  75                  80  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
                85                  90                  95      


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
            100                 105                 110         


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
        115                 120                 125             


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
    130                 135                 140                 


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
145                 150                 155                 160 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
                165                 170                 175     


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
            180                 185                 190         


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
        195                 200                 205             


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
    210                 215                 220                 


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
225                 230                 235                 240 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
                245                 250                 255     


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
            260                 265                 270         


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
        275                 280                 285             


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
    290                 295                 300                 


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
305                 310                 315                 320 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
                325                 330                 335     


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
            340                 345                 350         


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
        355                 360                 365             


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
    370                 375                 380                 


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
385                 390                 395                 400 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
                405                 410                 415     


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
            420                 425                 430         


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
        435                 440                 445             


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
    450                 455                 460                 


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
465                 470                 475                 480 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
                485                 490                 495     


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
            500                 505                 510         


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
        515                 520                 525             


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
    530                 535                 540                 


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
545                 550                 555                 560 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
                565                 570                 575     


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
            580                 585                 590         


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
        595                 600                 605             


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
    610                 615                 620                 


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
625                 630                 635                 640 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
                645                 650                 655     


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
            660                 665                 670         


Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
        675                 680                 685             


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 700                 


Val Gly Cys Ser Val Asp Phe Ser Lys 
705                 710             


<210>  494
<211>  452
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723)

<400>  494

Met Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
1               5                   10                  15      


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
            20                  25                  30          


Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly 
        35                  40                  45              


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
    50                  55                  60                  


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
65                  70                  75                  80  


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
                85                  90                  95      


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
            100                 105                 110         


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
        115                 120                 125             


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
    130                 135                 140                 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
145                 150                 155                 160 


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
                165                 170                 175     


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
            180                 185                 190         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        195                 200                 205             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    210                 215                 220                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
225                 230                 235                 240 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                245                 250                 255     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            260                 265                 270         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        275                 280                 285             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
    290                 295                 300                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
305                 310                 315                 320 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
                325                 330                 335     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            340                 345                 350         


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
        355                 360                 365             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    370                 375                 380                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
385                 390                 395                 400 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
                405                 410                 415     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            420                 425                 430         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        435                 440                 445             


Asp Phe Gly Ser 
    450         


<210>  495
<211>  709
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800)

<400>  495

Met Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp 
1               5                   10                  15      


Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
            20                  25                  30          


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
        35                  40                  45              


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
    50                  55                  60                  


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
65                  70                  75                  80  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
                85                  90                  95      


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
            100                 105                 110         


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
        115                 120                 125             


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
    130                 135                 140                 


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
145                 150                 155                 160 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
                165                 170                 175     


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
            180                 185                 190         


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
        195                 200                 205             


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
    210                 215                 220                 


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
225                 230                 235                 240 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
                245                 250                 255     


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
            260                 265                 270         


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
        275                 280                 285             


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
    290                 295                 300                 


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
305                 310                 315                 320 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
                325                 330                 335     


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly 
            340                 345                 350         


Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser 
    690                 695                 700                 


Val Asp Phe Ser Lys 
705                 


<210>  496
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719)

<400>  496

Met Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
1               5                   10                  15      


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
            20                  25                  30          


Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly 
        35                  40                  45              


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
    50                  55                  60                  


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
65                  70                  75                  80  


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
                85                  90                  95      


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
            100                 105                 110         


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
        115                 120                 125             


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met 
    130                 135                 140                 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
145                 150                 155                 160 


Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp 
                165                 170                 175     


Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu 
            180                 185                 190         


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
        195                 200                 205             


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
    210                 215                 220                 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
225                 230                 235                 240 


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
                245                 250                 255     


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
            260                 265                 270         


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
        275                 280                 285             


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu 
    290                 295                 300                 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
305                 310                 315                 320 


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr 
                325                 330                 335     


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
            340                 345                 350         


Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr 
        355                 360                 365             


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
    370                 375                 380                 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
385                 390                 395                 400 


Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His 
                405                 410                 415     


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
            420                 425                 430         


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
        435                 440                 445             


<210>  497
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  497
ggcgcagaua ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggca             54


<210>  498
<211>  54
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  498
ggcgcagaua cgagugucgg aauuguuggc cuccugcuga ccacagcuau ggca             54


<210>  499
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  499

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
            20                  25                  30  


<210>  500
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  500

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
            20                  25                  30  


<210>  501
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  501

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala 
            20                  25                  30  


<210>  502
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  502
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca                                        90


<210>  503
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  503
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca                                        90


<210>  504
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  504
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca                                        90


<210>  505
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  505
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg                                        90


<210>  506
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  506
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg                                        90


<210>  507
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  507
cggaucauca acgcccgcaa ggagcggaag cgccggggcg ccgacaccag caucgggauc       60

aucggccugc ugcucacgac cgccauggcg                                        90


<210>  508
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  508
cggaucauca acgcccggaa agagaagaag cgcagaggcg ccgauaccag cgugggcauu       60

gugggccugc ugcugacaac agccauggcc                                        90


<210>  509
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  509
cggaucauca acgcccggaa agagaagaag cgcagaggcg ccgauaccag cgugggcauu       60

gugggccugc ugcugacaac agccauggcc                                        90


<210>  510
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  510
cggaucauca acgcccggaa agagcggaag cggagaggcg ccgauaccag caucggcauc       60

aucggccugc ugcugaccac agccauggcc                                        90


<210>  511
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  511
cggaucauca acgcccggaa agagaagaag cgcagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugacaac cgccauggcc                                        90


<210>  512
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  512
cggaucauca acgcccggaa agagaagaag cgcagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugacaac cgccauggcc                                        90


<210>  513
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  513
cggaucauca acgcccggaa agagcggaag cggagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc                                        90


<210>  514
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  514
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc                                        90


<210>  515
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  515
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc                                        90


<210>  516
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) SS

<400>  516
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc                                        90


<210>  517
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) SS

<400>  517
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca                                        90


<210>  518
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) SS

<400>  518
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca                                        90


<210>  519
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C

<400>  519

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu 
                85                  90          


<210>  520
<211>  342
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS1

<400>  520

Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr Asn Asp Val Glu 
1               5                   10                  15      


Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu 
            20                  25                  30          


Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser 
        35                  40                  45              


Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser Val Glu Gly Glu 
    50                  55                  60                  


Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val 
65                  70                  75                  80  


Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val 
                85                  90                  95      


Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser His 
            100                 105                 110         


Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp 
        115                 120                 125             


Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp Asn Ser Phe Leu 
    130                 135                 140                 


Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys 
145                 150                 155                 160 


Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr 
                165                 170                 175     


Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu Gly Tyr Trp Ile 
            180                 185                 190         


Glu Ser Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile 
        195                 200                 205             


Glu Met Lys Thr Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp 
    210                 215                 220                 


Gly Ile Glu Glu Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro 
225                 230                 235                 240 


Leu Ser His His Asn Thr Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly 
                245                 250                 255     


Pro Trp His Ser Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly 
            260                 265                 270         


Thr Lys Val His Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu 
        275                 280                 285             


Arg Ser Thr Thr Ala Ser Gly Arg Val Ile Glu Glu Trp Cys Cys Arg 
    290                 295                 300                 


Glu Cys Thr Met Pro Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp 
305                 310                 315                 320 


Tyr Gly Met Glu Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val 
                325                 330                 335     


Arg Ser Met Val Thr Ala 
            340         


<210>  521
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C

<400>  521

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu 
                85                  90          


<210>  522
<211>  342
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS1

<400>  522

Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr Asn Asp Val Glu 
1               5                   10                  15      


Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu 
            20                  25                  30          


Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser 
        35                  40                  45              


Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser Val Glu Gly Glu 
    50                  55                  60                  


Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val 
65                  70                  75                  80  


Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val 
                85                  90                  95      


Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser Tyr 
            100                 105                 110         


Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp 
        115                 120                 125             


Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp Asn Ser Phe Leu 
    130                 135                 140                 


Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys 
145                 150                 155                 160 


Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr 
                165                 170                 175     


Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu Gly Tyr Trp Ile 
            180                 185                 190         


Glu Ser Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile 
        195                 200                 205             


Glu Met Lys Thr Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp 
    210                 215                 220                 


Gly Ile Glu Glu Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro 
225                 230                 235                 240 


Leu Ser His His Asn Thr Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly 
                245                 250                 255     


Pro Trp His Ser Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly 
            260                 265                 270         


Thr Lys Val His Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu 
        275                 280                 285             


Arg Ser Thr Thr Ala Ser Gly Arg Val Ile Glu Glu Trp Cys Cys Arg 
    290                 295                 300                 


Glu Cys Thr Met Pro Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp 
305                 310                 315                 320 


Tyr Gly Met Glu Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val 
                325                 330                 335     


Arg Ser Met Val Thr Ala 
            340         


<210>  523
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C

<400>  523

Met Lys Asn Pro Lys Glu Glu Ile Arg Arg Ile Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Asn Pro Leu Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu 
                85                  90          


<210>  524
<211>  342
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS1

<400>  524

Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Ile Tyr Asn Asp Val Glu 
1               5                   10                  15      


Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu 
            20                  25                  30          


Ala Ala Ala Val Lys Gln Ala Trp Glu Glu Gly Ile Cys Gly Ile Ser 
        35                  40                  45              


Ser Val Ser Arg Met Glu Asn Ile Met Trp Lys Ser Val Glu Gly Glu 
    50                  55                  60                  


Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val 
65                  70                  75                  80  


Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val 
                85                  90                  95      


Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser Tyr 
            100                 105                 110         


Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp 
        115                 120                 125             


Thr Leu Lys Glu Cys Pro Leu Glu His Arg Ala Trp Asn Ser Phe Leu 
    130                 135                 140                 


Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys 
145                 150                 155                 160 


Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr 
                165                 170                 175     


Ala Val Lys Gly Arg Glu Ala Ala His Ser Asp Leu Gly Tyr Trp Ile 
            180                 185                 190         


Glu Ser Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile 
        195                 200                 205             


Glu Met Lys Thr Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp 
    210                 215                 220                 


Gly Val Glu Glu Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro 
225                 230                 235                 240 


Leu Ser His His Asn Thr Arg Glu Gly Tyr Arg Thr Gln Val Lys Gly 
                245                 250                 255     


Pro Trp His Ser Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly 
            260                 265                 270         


Thr Lys Val Tyr Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu 
        275                 280                 285             


Arg Ser Thr Thr Ala Ser Gly Arg Val Ile Glu Glu Trp Cys Cys Arg 
    290                 295                 300                 


Glu Cys Thr Met Pro Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp 
305                 310                 315                 320 


Tyr Gly Met Glu Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val 
                325                 330                 335     


Arg Ser Met Val Thr Ala 
            340         


<210>  525
<211>  276
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) C CDS mRNA wild type

<400>  525
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcaauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcug                                276


<210>  526
<211>  1026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) NS1 CDS mRNA wild type

<400>  526
aaggagacga gaugcgguac agggguguuc gucuauaacg acguugaagc cuggagggac       60

agguacaagu accauccuga cuccccccgu agauuggcag cagcagucaa gcaagccugg      120

gaagauggua ucugcgggau cuccucuguu ucaagaaugg aaaacaucau guggagauca      180

guagaagggg agcucaacgc aauccuggaa gagaauggag uucaacugac ggucguugug      240

ggaucuguaa aaaaccccau guggagaggu ccacagagau ugcccgugcc ugugaacgag      300

cugccccacg gcuggaaggc uugggggaaa ucgcacuucg ucagagcagc aaagacaaau      360

aacagcuuug ucguggaugg ugacacacug aaggaaugcc cacucaaaca uagagcaugg      420

aacagcuuuc uuguggagga ucauggguuc gggguauuuc acacuagugu cuggcucaag      480

guuagagaag auuauucauu agagugugau ccagccguua uuggaacagc uguuaaggga      540

aaggaggcug uacacaguga ucuaggcuac uggauugaga gugagaagaa ugacacaugg      600

aggcugaaga gggcccaucu gaucgagaug aaaacaugug aauggccaaa gucccacaca      660

uuguggacag auggaauaga agagagugau cugaucauac ccaagucuuu agcugggcca      720

cucagccauc acaauaccag agagggcuac aggacccaaa ugaaagggcc auggcacagu      780

gaagagcuug aaauucgguu ugaggaaugc ccaggcacua agguccacgu ggaggaaaca      840

uguggaacaa gaggaccauc ucugagauca accacugcaa gcggaagggu gaucgaggaa      900

uggugcugca gggagugcac aaugccccca cugucguucc gggcuaaaga uggcuguugg      960

uauggaaugg agauaaggcc caggaaagaa ccagaaagca acuuaguaag gucaauggug     1020

acugca                                                                1026


<210>  527
<211>  276
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) C CDS mRNA wild type

<400>  527
augaaaaacc caaaaaagaa auccggagga uuccggauug ucaauaugcu aaaacgcgga       60

guagcccgug ugagccccuu ugggggcuug aagaggcugc cagccggacu ucugcugggu      120

caugggccca ucaggauggu cuuggcgauu cuagccuuuu ugagauucac ggcaaucaag      180

ccaucacugg gucucaucaa uagauggggu ucagugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuggcugcc augcug                                276


<210>  528
<211>  1026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) NS1 CDS mRNA wild type

<400>  528
aaggagacga gaugcgguac agggguguuc gucuauaacg acguugaagc cuggagggac       60

agguacaagu accauccuga cuccccccgu agauuggcag cagcagucaa gcaagccugg      120

gaagauggua ucugcgggau cuccucuguu ucaagaaugg aaaacaucau guggagauca      180

guagaagggg agcucaacgc aauccuggaa gagaauggag uucaacugac ggucguugug      240

ggaucuguaa aaaaccccau guggagaggu ccacagagau ugcccgugcc ugugaacgag      300

cugccccacg gcuggaaggc uugggggaaa ucguacuucg ucagagcagc aaagacaaau      360

aacagcuuug ucguggaugg ugacacacug aaggaaugcc cacucaaaca uagagcaugg      420

aacagcuuuc uuguggagga ucauggguuc gggguauuuc acacuagugu cuggcucaag      480

guuagagaag auuauucauu agagugugau ccagccguua uuggaacagc uguuaaggga      540

aaggaggcug uacacaguga ucuaggcuac uggauugaga gugagaagaa ugacacaugg      600

aggcugaaga gggcccaucu gaucgagaug aaaacaugug aauggccaaa gucccacaca      660

uuguggacag auggaauaga agagagugau cugaucauac ccaagucuuu agcugggcca      720

cucagccauc acaauaccag agagggcuac aggacccaaa ugaaagggcc auggcacagu      780

gaagagcuug aaauucgguu ugaggaaugc ccaggcacua agguccacgu ggaggaaaca      840

uguggaacga gaggaccauc ucugagauca accacugcaa gcggaagggu gaucgaggaa      900

uggugcugca gggagugcac aaugccccca cugucguucc gggcuaaaga uggcuguugg      960

uauggaaugg agauaaggcc caggaaagaa ccagaaagca acuuaguaag gucaauggug     1020

acugca                                                                1026


<210>  529
<211>  276
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) C CDS mRNA wild type

<400>  529
augaaaaacc ccaaagaaga aauccggagg auccggauug ucaauaugcu aaaacgcgga       60

guagcccgug uaaaccccuu gggagguuug aagagguugc cagccggacu ucugcugggu      120

cauggaccca ucagaauggu uuuggcgaua cuagccuuuu ugagauuuac agcaaucaag      180

ccaucacugg gccuuaucaa cagauggggu uccgugggga aaaaagaggc uauggaaaua      240

auaaagaagu ucaagaaaga ucuugcugcc auguug                                276


<210>  530
<211>  1026
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) NS1 CDS mRNA wild type

<400>  530
aaggaaacga gauguggcac ggggguauuc aucuauaaug auguugaagc cuggagggac       60

cgguacaagu accauccuga cuccccccgc agauuggcag cagcagucaa gcaggccugg      120

gaagagggga ucugugggau cucauccguu ucaagaaugg aaaacaucau guggaaauca      180

guagaagggg agcucaaugc uauccuagag gagaauggag uucaacugac aguuguugug      240

ggaucuguaa aaaaccccau guggagaggu ccacaaagau ugccagugcc ugugaaugag      300

cugccccaug gcuggaaagc cugggggaaa ucguauuuug uuagggcggc aaagaccaac      360

aacaguuuug uugucgacgg ugacacacug aaggaauguc cgcuugagca cagagcaugg      420

aauaguuuuc uuguggagga ucacggguuu ggagucuucc acaccagugu cuggcuuaag      480

gucagagaag auuacucauu agaaugugac ccagccguca uaggaacagc uguuaaggga      540

agggaggccg cgcacaguga ucugggcuau uggauugaaa gugaaaagaa ugacacaugg      600

aggcugaaga gggcccaccu gauugagaug aaaacaugug aauggccaaa gucucacaca      660

uuguggacag auggaguaga agaaagugau cuuaucauac ccaagucuuu agcuggucca      720

cucagccacc acaacaccag agaggguuac agaacccaag ugaaagggcc auggcacagu      780

gaagagcuug aaauccgguu ugaggaaugu ccaggcacca agguuuacgu ggaggagaca      840

ugcggaacua gaggaccauc ucugagauca acuacugcaa guggaagggu cauugaggaa      900

uggugcugua gggaaugcac aaugccccca cuaucguuuc gagcaaaaga cggcugcugg      960

uauggaaugg agauaaggcc caggaaagaa ccagagagca acuuagugag gucaauggug     1020

acagcg                                                                1026


<210>  531
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak

<400>  531
gccgccacca tgg                                                          13


<210>  532
<211>  13
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Kozak mRNA

<400>  532
gccgccacca ugg                                                          13


<210>  533
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804)

<400>  533

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  534
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804)

<400>  534

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  535
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800)

<400>  535

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr 
            340                 345                 350         


Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
        355                 360                 365             


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
    370                 375                 380                 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
385                 390                 395                 400 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
                405                 410                 415     


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
            420                 425                 430         


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
        435                 440                 445             


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
    450                 455                 460                 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser 
465                 470                 475                 480 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
                485                 490                 495     


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
            500                 505                 510         


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
        515                 520                 525             


Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln 
    530                 535                 540                 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
545                 550                 555                 560 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
                565                 570                 575     


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
            580                 585                 590         


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
        595                 600                 605             


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
    610                 615                 620                 


Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His 
625                 630                 635                 640 


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
                645                 650                 655     


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn 
            660                 665                 670         


Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val 
        675                 680                 685             


Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
    690                 695                 700                 


Asp Phe Ser Lys 
705             


<210>  536
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804)

<400>  536

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  537
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)

<400>  537

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  538
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)

<400>  538

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  539
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)

<400>  539

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly 
                325                 330                 335     


Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser 
            340                 345                 350         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        355                 360                 365             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    370                 375                 380                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
385                 390                 395                 400 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                405                 410                 415     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            420                 425                 430         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        435                 440                 445             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe 
    450                 455                 460                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
465                 470                 475                 480 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val 
                485                 490                 495     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            500                 505                 510         


Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met 
        515                 520                 525             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    530                 535                 540                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
545                 550                 555                 560 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr 
                565                 570                 575     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            580                 585                 590         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        595                 600                 605             


Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile 
    610                 615                 620                 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
625                 630                 635                 640 


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu 
                645                 650                 655     


Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly 
            660                 665                 670         


Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680             


<210>  540
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)

<400>  540

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  541
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723)

<400>  541

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  542
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723)

<400>  542

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  543
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719)

<400>  543

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu 
                165                 170                 175     


Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala 
            180                 185                 190         


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
        195                 200                 205             


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
    210                 215                 220                 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
225                 230                 235                 240 


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
                245                 250                 255     


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
            260                 265                 270         


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
        275                 280                 285             


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys 
    290                 295                 300                 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
305                 310                 315                 320 


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu 
                325                 330                 335     


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
            340                 345                 350         


Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
        355                 360                 365             


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
    370                 375                 380                 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
385                 390                 395                 400 


Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg 
                405                 410                 415     


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
            420                 425                 430         


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
        435                 440                 445         


<210>  544
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723)

<400>  544

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  545
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S)

<400>  545

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Ser Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  546
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S)

<400>  546

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Ser Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  547
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S)

<400>  547

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Ser Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly 
                325                 330                 335     


Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser 
            340                 345                 350         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        355                 360                 365             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    370                 375                 380                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
385                 390                 395                 400 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                405                 410                 415     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            420                 425                 430         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        435                 440                 445             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe 
    450                 455                 460                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
465                 470                 475                 480 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val 
                485                 490                 495     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            500                 505                 510         


Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met 
        515                 520                 525             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    530                 535                 540                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
545                 550                 555                 560 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr 
                565                 570                 575     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            580                 585                 590         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        595                 600                 605             


Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile 
    610                 615                 620                 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
625                 630                 635                 640 


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu 
                645                 650                 655     


Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly 
            660                 665                 670         


Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680             


<210>  548
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S)

<400>  548

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Ser Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  549
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q)

<400>  549

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Gln Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  550
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q)

<400>  550

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Gln Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  551
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q)

<400>  551

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Gln Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  552
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500)

<400>  552

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Leu Gly Lys 
            580                 585                 590         


Ala Phe Ser Thr Thr Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Ile Gly Gly Val Phe Asn Ser Ile 
    610                 615                 620                 


Gly Arg Ala Val His Gln Val Phe Gly Gly Ala Phe Arg Thr Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Ile Thr Gln Gly Leu Met Gly Ala Leu Leu Leu 
                645                 650                 655     


Trp Met Gly Val Asn Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe Leu 
            660                 665                 670         


Ala Thr Gly Gly Val Leu Val Phe Leu Ala Thr Asn Val His Ala 
        675                 680                 685         


<210>  553
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500)

<400>  553

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Leu Gly Lys 
            580                 585                 590         


Ala Phe Ser Thr Thr Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Ile Gly Gly Val Phe Asn Ser Ile 
    610                 615                 620                 


Gly Arg Ala Val His Gln Val Phe Gly Gly Ala Phe Arg Thr Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Ile Thr Gln Gly Leu Met Gly Ala Leu Leu Leu 
                645                 650                 655     


Trp Met Gly Val Asn Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe Leu 
            660                 665                 670         


Ala Thr Gly Gly Val Leu Val Phe Leu Ala Thr Asn Val His Ala 
        675                 680                 685         


<210>  554
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500)

<400>  554

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly 
                325                 330                 335     


Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser 
            340                 345                 350         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        355                 360                 365             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    370                 375                 380                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
385                 390                 395                 400 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                405                 410                 415     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            420                 425                 430         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        435                 440                 445             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe 
    450                 455                 460                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
465                 470                 475                 480 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val 
                485                 490                 495     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            500                 505                 510         


Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met 
        515                 520                 525             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    530                 535                 540                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
545                 550                 555                 560 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr 
                565                 570                 575     


His His Trp His Arg Ser Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr 
            580                 585                 590         


Thr Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp 
        595                 600                 605             


Asp Phe Gly Ser Ile Gly Gly Val Phe Asn Ser Ile Gly Arg Ala Val 
    610                 615                 620                 


His Gln Val Phe Gly Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser 
625                 630                 635                 640 


Trp Ile Thr Gln Gly Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val 
                645                 650                 655     


Asn Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly 
            660                 665                 670         


Val Leu Val Phe Leu Ala Thr Asn Val His Ala 
        675                 680             


<210>  555
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500)

<400>  555

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Leu Gly Lys 
            580                 585                 590         


Ala Phe Ser Thr Thr Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Ile Gly Gly Val Phe Asn Ser Ile 
    610                 615                 620                 


Gly Arg Ala Val His Gln Val Phe Gly Gly Ala Phe Arg Thr Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Ile Thr Gln Gly Leu Met Gly Ala Leu Leu Leu 
                645                 650                 655     


Trp Met Gly Val Asn Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe Leu 
            660                 665                 670         


Ala Thr Gly Gly Val Leu Val Phe Leu Ala Thr Asn Val His Ala 
        675                 680                 685         


<210>  556
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804)

<400>  556

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  557
<211>  711
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  557

Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser 
1               5                   10                  15      


Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val 
            20                  25                  30          


Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala 
        35                  40                  45              


Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr 
    50                  55                  60                  


Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr 
65                  70                  75                  80  


Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys 
                85                  90                  95      


Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His 
            100                 105                 110         


Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser 
        115                 120                 125             


His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser 
    130                 135                 140                 


Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg 
145                 150                 155                 160 


Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly 
                165                 170                 175     


Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile 
            180                 185                 190         


Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe 
        195                 200                 205             


Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His 
    210                 215                 220                 


Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile 
225                 230                 235                 240 


Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr 
                245                 250                 255     


Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro 
            260                 265                 270         


Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val 
        275                 280                 285             


Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu 
    290                 295                 300                 


Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys 
305                 310                 315                 320 


Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile 
                325                 330                 335     


Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp 
            340                 345                 350         


Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro 
        355                 360                 365             


Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly 
    370                 375                 380                 


Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr 
385                 390                 395                 400 


Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His 
                405                 410                 415     


Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His 
            420                 425                 430         


Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys 
        435                 440                 445             


Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr 
    450                 455                 460                 


Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg 
465                 470                 475                 480 


Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg 
                485                 490                 495     


Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr 
            500                 505                 510         


Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln 
        515                 520                 525             


Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val 
    530                 535                 540                 


Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro 
545                 550                 555                 560 


Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp 
                565                 570                 575     


Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys 
            580                 585                 590         


Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe 
        595                 600                 605             


Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr 
    610                 615                 620                 


Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys 
625                 630                 635                 640 


Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly 
                645                 650                 655     


Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu 
            660                 665                 670         


Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu 
        675                 680                 685             


Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly 
    690                 695                 700                 


Cys Ser Val Asp Phe Ser Lys 
705                 710     


<210>  558
<211>  710
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  558

Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val 
1               5                   10                  15      


Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr 
            20                  25                  30          


Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly 
        35                  40                  45              


Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile 
    50                  55                  60                  


Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu 
65                  70                  75                  80  


Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp 
                85                  90                  95      


Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys 
            100                 105                 110         


Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His 
        115                 120                 125             


Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg 
    130                 135                 140                 


Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn 
145                 150                 155                 160 


Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser 
                165                 170                 175     


Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala 
            180                 185                 190         


Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val 
        195                 200                 205             


Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly 
    210                 215                 220                 


Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu 
225                 230                 235                 240 


Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys 
                245                 250                 255     


Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr 
            260                 265                 270         


Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys 
        275                 280                 285             


Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe 
    290                 295                 300                 


Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys 
305                 310                 315                 320 


Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met 
                325                 330                 335     


Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr 
            340                 345                 350         


Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn 
        355                 360                 365             


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
    370                 375                 380                 


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
385                 390                 395                 400 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
                405                 410                 415     


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
            420                 425                 430         


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
        435                 440                 445             


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
    450                 455                 460                 


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
465                 470                 475                 480 


Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
                485                 490                 495     


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
            500                 505                 510         


Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
        515                 520                 525             


Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp 
    530                 535                 540                 


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
545                 550                 555                 560 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
                565                 570                 575     


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile 
            580                 585                 590         


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
        595                 600                 605             


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
    610                 615                 620                 


Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly 
625                 630                 635                 640 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
                645                 650                 655     


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly 
            660                 665                 670         


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly 
        675                 680                 685             


Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 700                 


Ser Val Asp Phe Ser Lys 
705                 710 


<210>  559
<211>  709
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  559

Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly 
1               5                   10                  15      


Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser 
    690                 695                 700                 


Val Asp Phe Ser Lys 
705                 


<210>  560
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  560

Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
1               5                   10                  15      


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
            20                  25                  30          


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
        35                  40                  45              


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
    50                  55                  60                  


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
65                  70                  75                  80  


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
                85                  90                  95      


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
            100                 105                 110         


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
        115                 120                 125             


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
    130                 135                 140                 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
145                 150                 155                 160 


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
                165                 170                 175     


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
            180                 185                 190         


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
        195                 200                 205             


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
    210                 215                 220                 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
225                 230                 235                 240 


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
                245                 250                 255     


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
            260                 265                 270         


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
        275                 280                 285             


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
    290                 295                 300                 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
305                 310                 315                 320 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
                325                 330                 335     


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
            340                 345                 350         


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
        355                 360                 365             


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
    370                 375                 380                 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
385                 390                 395                 400 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
                405                 410                 415     


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
            420                 425                 430         


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
        435                 440                 445             


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
    450                 455                 460                 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
465                 470                 475                 480 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
                485                 490                 495     


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
            500                 505                 510         


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
        515                 520                 525             


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
    530                 535                 540                 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
545                 550                 555                 560 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
                565                 570                 575     


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
            580                 585                 590         


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
        595                 600                 605             


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
    610                 615                 620                 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
625                 630                 635                 640 


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
                645                 650                 655     


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
            660                 665                 670         


Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
        675                 680                 685             


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
    690                 695                 700                 


Asp Phe Ser Lys 
705             


<210>  561
<211>  707
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  561

Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val 
1               5                   10                  15      


Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly 
            20                  25                  30          


Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile 
        35                  40                  45              


Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met 
    50                  55                  60                  


Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met 
65                  70                  75                  80  


Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr 
                85                  90                  95      


Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu 
            100                 105                 110         


Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg 
        115                 120                 125             


Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr 
    130                 135                 140                 


Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe 
145                 150                 155                 160 


Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser 
                165                 170                 175     


Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr 
            180                 185                 190         


Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met 
        195                 200                 205             


Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val 
    210                 215                 220                 


Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr 
225                 230                 235                 240 


Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala 
                245                 250                 255     


Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu 
            260                 265                 270         


Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr 
        275                 280                 285             


Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly 
    290                 295                 300                 


Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly 
305                 310                 315                 320 


Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val 
                325                 330                 335     


His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu 
            340                 345                 350         


Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg 
        355                 360                 365             


Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu 
    370                 375                 380                 


Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn 
385                 390                 395                 400 


Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu 
                405                 410                 415     


Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys 
            420                 425                 430         


Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val 
        435                 440                 445             


Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly 
    450                 455                 460                 


Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly 
465                 470                 475                 480 


His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val 
                485                 490                 495     


Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala 
            500                 505                 510         


Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr 
        515                 520                 525             


Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr 
    530                 535                 540                 


Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu 
545                 550                 555                 560 


Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly 
                565                 570                 575     


Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His 
            580                 585                 590         


Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val 
        595                 600                 605             


Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe 
    610                 615                 620                 


Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln 
625                 630                 635                 640 


Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe 
                645                 650                 655     


Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr 
            660                 665                 670         


Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu 
        675                 680                 685             


Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp 
    690                 695                 700                 


Phe Ser Lys 
705         


<210>  562
<211>  706
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  562

Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly 
1               5                   10                  15      


Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser 
            20                  25                  30          


Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser 
        35                  40                  45              


Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp 
    50                  55                  60                  


Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu 
65                  70                  75                  80  


Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr 
                85                  90                  95      


Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala 
            100                 105                 110         


Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys 
        115                 120                 125             


Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys 
    130                 135                 140                 


His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala 
145                 150                 155                 160 


Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln 
                165                 170                 175     


Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
            180                 185                 190         


Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
        195                 200                 205             


Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr 
    210                 215                 220                 


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
225                 230                 235                 240 


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
                245                 250                 255     


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
            260                 265                 270         


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
        275                 280                 285             


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
    290                 295                 300                 


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
305                 310                 315                 320 


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
                325                 330                 335     


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
            340                 345                 350         


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
        355                 360                 365             


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
    370                 375                 380                 


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
385                 390                 395                 400 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
                405                 410                 415     


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
            420                 425                 430         


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
        435                 440                 445             


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
    450                 455                 460                 


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
465                 470                 475                 480 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
                485                 490                 495     


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
            500                 505                 510         


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
        515                 520                 525             


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
    530                 535                 540                 


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
545                 550                 555                 560 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
                565                 570                 575     


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
            580                 585                 590         


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
        595                 600                 605             


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
    610                 615                 620                 


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
625                 630                 635                 640 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
                645                 650                 655     


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
            660                 665                 670         


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
        675                 680                 685             


Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe 
    690                 695                 700                 


Ser Lys 
705     


<210>  563
<211>  705
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  563

Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu 
1               5                   10                  15      


Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
        595                 600                 605             


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe 
625                 630                 635                 640 


Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln 
                645                 650                 655     


Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn 
            660                 665                 670         


Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe 
        675                 680                 685             


Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser 
    690                 695                 700                 


Lys 
705 


<210>  564
<211>  704
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  564

Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu 
1               5                   10                  15      


Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        595                 600                 605             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    610                 615                 620                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
625                 630                 635                 640 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                645                 650                 655     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly 
            660                 665                 670         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        675                 680                 685             


Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695                 700                 


<210>  565
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  565

Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu 
1               5                   10                  15      


Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr 
            20                  25                  30          


Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr 
        35                  40                  45              


Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His 
    50                  55                  60                  


Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly 
65                  70                  75                  80  


Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp 
                85                  90                  95      


Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser 
            100                 105                 110         


Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr 
        115                 120                 125             


Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile 
    130                 135                 140                 


Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala 
145                 150                 155                 160 


Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile 
                165                 170                 175     


Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys 
            180                 185                 190         


Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr 
        195                 200                 205             


Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala 
    210                 215                 220                 


Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser 
225                 230                 235                 240 


Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp 
                245                 250                 255     


Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp 
            260                 265                 270         


Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg 
        275                 280                 285             


Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr 
    290                 295                 300                 


Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln 
305                 310                 315                 320 


Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln 
                325                 330                 335     


His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn 
            340                 345                 350         


Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr 
        355                 360                 365             


Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly 
    370                 375                 380                 


Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp 
385                 390                 395                 400 


Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala 
                405                 410                 415     


Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val 
            420                 425                 430         


Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly 
        435                 440                 445             


Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala 
    450                 455                 460                 


Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys 
465                 470                 475                 480 


Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu 
                485                 490                 495     


Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His 
            500                 505                 510         


Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys 
        515                 520                 525             


Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val 
    530                 535                 540                 


Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn 
545                 550                 555                 560 


Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile 
                565                 570                 575     


Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser 
            580                 585                 590         


Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys 
        595                 600                 605             


Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 
    610                 615                 620                 


Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala 
625                 630                 635                 640 


Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu 
                645                 650                 655     


Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser 
            660                 665                 670         


Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser 
        675                 680                 685             


Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695                 700             


<210>  566
<211>  702
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  566

Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr 
1               5                   10                  15      


Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met 
            20                  25                  30          


Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr 
        35                  40                  45              


Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met 
    50                  55                  60                  


Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val 
65                  70                  75                  80  


Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val 
                85                  90                  95      


Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg 
            100                 105                 110         


Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg 
        115                 120                 125             


Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg 
    130                 135                 140                 


Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala 
145                 150                 155                 160 


Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr 
                165                 170                 175     


Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile 
            180                 185                 190         


Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp 
        195                 200                 205             


Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln 
    210                 215                 220                 


Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn 
225                 230                 235                 240 


Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met 
                245                 250                 255     


Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys 
            260                 265                 270         


Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly 
        275                 280                 285             


Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys 
    290                 295                 300                 


Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro 
305                 310                 315                 320 


Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His 
                325                 330                 335     


Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg 
            340                 345                 350         


Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
        355                 360                 365             


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 
    370                 375                 380                 


Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 
385                 390                 395                 400 


Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 
                405                 410                 415     


Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu 
            420                 425                 430         


Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 
        435                 440                 445             


Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 
    450                 455                 460                 


Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg 
465                 470                 475                 480 


Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 
                485                 490                 495     


Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly 
            500                 505                 510         


Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 
        515                 520                 525             


Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 
    530                 535                 540                 


Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 
545                 550                 555                 560 


Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 
                565                 570                 575     


Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly 
            580                 585                 590         


Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 
        595                 600                 605             


Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly 
    610                 615                 620                 


Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala 
625                 630                 635                 640 


Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile 
                645                 650                 655     


Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile 
            660                 665                 670         


Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr 
        675                 680                 685             


Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695                 700         


<210>  567
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  567

Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr 
1               5                   10                  15      


Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr 
            20                  25                  30          


Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu 
        35                  40                  45              


Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys 
    50                  55                  60                  


Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu 
65                  70                  75                  80  


Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val 
                85                  90                  95      


Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
            100                 105                 110         


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
        115                 120                 125             


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
    130                 135                 140                 


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
145                 150                 155                 160 


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
                165                 170                 175     


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
            180                 185                 190         


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
        195                 200                 205             


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
    210                 215                 220                 


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
225                 230                 235                 240 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
                245                 250                 255     


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
            260                 265                 270         


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
        275                 280                 285             


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
    290                 295                 300                 


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
305                 310                 315                 320 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
                325                 330                 335     


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
            340                 345                 350         


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
        515                 520                 525             


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
    610                 615                 620                 


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695                 700     


<210>  568
<211>  700
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  568

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              


Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695                 700 


<210>  569
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  569

Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met 
1               5                   10                  15      


Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp 
            20                  25                  30          


Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met 
        35                  40                  45              


Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala 
    50                  55                  60                  


Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp 
65                  70                  75                  80  


Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly 
                85                  90                  95      


Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val 
            100                 105                 110         


Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr 
        115                 120                 125             


Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn 
    130                 135                 140                 


Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala 
145                 150                 155                 160 


Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met 
                165                 170                 175     


Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser 
            180                 185                 190         


Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile 
        195                 200                 205             


Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro 
    210                 215                 220                 


Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu 
225                 230                 235                 240 


Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp 
                245                 250                 255     


Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp 
            260                 265                 270         


Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn 
        275                 280                 285             


Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe 
    290                 295                 300                 


Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu 
305                 310                 315                 320 


Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met 
                325                 330                 335     


Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val 
            340                 345                 350         


Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe 
        355                 360                 365             


Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser 
    370                 375                 380                 


Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys 
385                 390                 395                 400 


Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr 
                405                 410                 415     


Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp 
            420                 425                 430         


Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly 
        435                 440                 445             


Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly 
    450                 455                 460                 


Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met 
465                 470                 475                 480 


Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala 
                485                 490                 495     


Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr 
            500                 505                 510         


Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala 
        515                 520                 525             


Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile 
    530                 535                 540                 


Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met 
545                 550                 555                 560 


Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val 
                565                 570                 575     


Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile 
            580                 585                 590         


Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val 
        595                 600                 605             


Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn 
    610                 615                 620                 


Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser 
625                 630                 635                 640 


Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu 
                645                 650                 655     


Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met 
            660                 665                 670         


Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser 
        675                 680                 685             


Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695                 


<210>  570
<211>  698
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  570

Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
1               5                   10                  15      


Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
    610                 615                 620                 


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
                645                 650                 655     


Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
            660                 665                 670         


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685             


Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695             


<210>  571
<211>  697
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  571

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
        675                 680                 685             


Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695         


<210>  572
<211>  697
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  572

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Ala Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
        675                 680                 685             


Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695         


<210>  573
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  573

Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr 
1               5                   10                  15      


Ala Met Ala Ala Ala Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr 
            20                  25                  30          


Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu 
        35                  40                  45              


Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys 
    50                  55                  60                  


Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu 
65                  70                  75                  80  


Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val 
                85                  90                  95      


Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
            100                 105                 110         


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
        115                 120                 125             


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
    130                 135                 140                 


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
145                 150                 155                 160 


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
                165                 170                 175     


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
            180                 185                 190         


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
        195                 200                 205             


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
    210                 215                 220                 


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
225                 230                 235                 240 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
                245                 250                 255     


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
            260                 265                 270         


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
        275                 280                 285             


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
    290                 295                 300                 


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
305                 310                 315                 320 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
                325                 330                 335     


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
            340                 345                 350         


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
        515                 520                 525             


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
    610                 615                 620                 


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe Ser Lys 
    690                 695                 700     


<210>  574
<211>  709
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  574

Arg Gly Leu Ser Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val 
1               5                   10                  15      


Gln Phe Leu Ile Leu Gly Met Leu Leu Met Thr Gly Gly Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser 
    690                 695                 700                 


Val Asp Phe Ser Lys 
705                 


<210>  575
<211>  709
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  575

Arg Gly Leu Ser Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val 
1               5                   10                  15      


Gln Phe Leu Ile Leu Gly Met Leu Leu Met Thr Gly Ala Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser 
    690                 695                 700                 


Val Asp Phe Ser Lys 
705                 


<210>  576
<211>  706
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  576

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys 
705     


<210>  577
<211>  705
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  577

Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser 
1               5                   10                  15      


Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val 
            20                  25                  30          


Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala 
        35                  40                  45              


Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr 
    50                  55                  60                  


Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr 
65                  70                  75                  80  


Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys 
                85                  90                  95      


Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His 
            100                 105                 110         


Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser 
        115                 120                 125             


His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser 
    130                 135                 140                 


Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg 
145                 150                 155                 160 


Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly 
                165                 170                 175     


Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile 
            180                 185                 190         


Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe 
        195                 200                 205             


Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His 
    210                 215                 220                 


Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile 
225                 230                 235                 240 


Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr 
                245                 250                 255     


Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro 
            260                 265                 270         


Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val 
        275                 280                 285             


Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu 
    290                 295                 300                 


Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys 
305                 310                 315                 320 


Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile 
                325                 330                 335     


Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp 
            340                 345                 350         


Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro 
        355                 360                 365             


Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly 
    370                 375                 380                 


Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr 
385                 390                 395                 400 


Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His 
                405                 410                 415     


Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His 
            420                 425                 430         


Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys 
        435                 440                 445             


Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr 
    450                 455                 460                 


Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg 
465                 470                 475                 480 


Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg 
                485                 490                 495     


Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr 
            500                 505                 510         


Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln 
        515                 520                 525             


Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val 
    530                 535                 540                 


Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro 
545                 550                 555                 560 


Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp 
                565                 570                 575     


Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys 
            580                 585                 590         


Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe 
        595                 600                 605             


Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr 
    610                 615                 620                 


Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys 
625                 630                 635                 640 


Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly 
                645                 650                 655     


Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu 
            660                 665                 670         


Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu 
        675                 680                 685             


Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly 
    690                 695                 700                 


Cys 
705 


<210>  578
<211>  704
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  578

Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val 
1               5                   10                  15      


Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr 
            20                  25                  30          


Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly 
        35                  40                  45              


Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile 
    50                  55                  60                  


Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu 
65                  70                  75                  80  


Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp 
                85                  90                  95      


Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys 
            100                 105                 110         


Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His 
        115                 120                 125             


Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg 
    130                 135                 140                 


Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn 
145                 150                 155                 160 


Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser 
                165                 170                 175     


Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala 
            180                 185                 190         


Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val 
        195                 200                 205             


Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly 
    210                 215                 220                 


Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu 
225                 230                 235                 240 


Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys 
                245                 250                 255     


Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr 
            260                 265                 270         


Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys 
        275                 280                 285             


Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe 
    290                 295                 300                 


Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys 
305                 310                 315                 320 


Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met 
                325                 330                 335     


Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr 
            340                 345                 350         


Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn 
        355                 360                 365             


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
    370                 375                 380                 


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
385                 390                 395                 400 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
                405                 410                 415     


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
            420                 425                 430         


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
        435                 440                 445             


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
    450                 455                 460                 


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
465                 470                 475                 480 


Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
                485                 490                 495     


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
            500                 505                 510         


Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
        515                 520                 525             


Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp 
    530                 535                 540                 


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
545                 550                 555                 560 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
                565                 570                 575     


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile 
            580                 585                 590         


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
        595                 600                 605             


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
    610                 615                 620                 


Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly 
625                 630                 635                 640 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
                645                 650                 655     


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly 
            660                 665                 670         


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly 
        675                 680                 685             


Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 700                 


<210>  579
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  579

Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly 
1               5                   10                  15      


Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 700             


<210>  580
<211>  702
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  580

Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
1               5                   10                  15      


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
            20                  25                  30          


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
        35                  40                  45              


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
    50                  55                  60                  


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
65                  70                  75                  80  


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
                85                  90                  95      


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
            100                 105                 110         


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
        115                 120                 125             


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
    130                 135                 140                 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
145                 150                 155                 160 


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
                165                 170                 175     


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
            180                 185                 190         


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
        195                 200                 205             


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
    210                 215                 220                 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
225                 230                 235                 240 


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
                245                 250                 255     


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
            260                 265                 270         


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
        275                 280                 285             


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
    290                 295                 300                 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
305                 310                 315                 320 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
                325                 330                 335     


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
            340                 345                 350         


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
        355                 360                 365             


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
    370                 375                 380                 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
385                 390                 395                 400 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
                405                 410                 415     


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
            420                 425                 430         


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
        435                 440                 445             


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
    450                 455                 460                 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
465                 470                 475                 480 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
                485                 490                 495     


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
            500                 505                 510         


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
        515                 520                 525             


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
    530                 535                 540                 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
545                 550                 555                 560 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
                565                 570                 575     


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
            580                 585                 590         


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
        595                 600                 605             


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
    610                 615                 620                 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
625                 630                 635                 640 


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
                645                 650                 655     


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
            660                 665                 670         


Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
        675                 680                 685             


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 700         


<210>  581
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  581

Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val 
1               5                   10                  15      


Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly 
            20                  25                  30          


Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile 
        35                  40                  45              


Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met 
    50                  55                  60                  


Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met 
65                  70                  75                  80  


Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr 
                85                  90                  95      


Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu 
            100                 105                 110         


Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg 
        115                 120                 125             


Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr 
    130                 135                 140                 


Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe 
145                 150                 155                 160 


Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser 
                165                 170                 175     


Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr 
            180                 185                 190         


Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met 
        195                 200                 205             


Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val 
    210                 215                 220                 


Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr 
225                 230                 235                 240 


Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala 
                245                 250                 255     


Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu 
            260                 265                 270         


Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr 
        275                 280                 285             


Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly 
    290                 295                 300                 


Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly 
305                 310                 315                 320 


Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val 
                325                 330                 335     


His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu 
            340                 345                 350         


Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg 
        355                 360                 365             


Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu 
    370                 375                 380                 


Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn 
385                 390                 395                 400 


Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu 
                405                 410                 415     


Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys 
            420                 425                 430         


Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val 
        435                 440                 445             


Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly 
    450                 455                 460                 


Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly 
465                 470                 475                 480 


His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val 
                485                 490                 495     


Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala 
            500                 505                 510         


Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr 
        515                 520                 525             


Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr 
    530                 535                 540                 


Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu 
545                 550                 555                 560 


Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly 
                565                 570                 575     


Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His 
            580                 585                 590         


Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val 
        595                 600                 605             


Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe 
    610                 615                 620                 


Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln 
625                 630                 635                 640 


Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe 
                645                 650                 655     


Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr 
            660                 665                 670         


Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu 
        675                 680                 685             


Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 700     


<210>  582
<211>  700
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  582

Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly 
1               5                   10                  15      


Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser 
            20                  25                  30          


Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser 
        35                  40                  45              


Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp 
    50                  55                  60                  


Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu 
65                  70                  75                  80  


Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr 
                85                  90                  95      


Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala 
            100                 105                 110         


Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys 
        115                 120                 125             


Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys 
    130                 135                 140                 


His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala 
145                 150                 155                 160 


Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln 
                165                 170                 175     


Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
            180                 185                 190         


Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
        195                 200                 205             


Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr 
    210                 215                 220                 


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
225                 230                 235                 240 


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
                245                 250                 255     


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
            260                 265                 270         


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
        275                 280                 285             


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
    290                 295                 300                 


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
305                 310                 315                 320 


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
                325                 330                 335     


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
            340                 345                 350         


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
        355                 360                 365             


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
    370                 375                 380                 


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
385                 390                 395                 400 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
                405                 410                 415     


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
            420                 425                 430         


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
        435                 440                 445             


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
    450                 455                 460                 


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
465                 470                 475                 480 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
                485                 490                 495     


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
            500                 505                 510         


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
        515                 520                 525             


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
    530                 535                 540                 


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
545                 550                 555                 560 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
                565                 570                 575     


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
            580                 585                 590         


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
        595                 600                 605             


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
    610                 615                 620                 


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
625                 630                 635                 640 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
                645                 650                 655     


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
            660                 665                 670         


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
        675                 680                 685             


Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 700 


<210>  583
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  583

Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu 
1               5                   10                  15      


Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
        595                 600                 605             


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe 
625                 630                 635                 640 


Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln 
                645                 650                 655     


Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn 
            660                 665                 670         


Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe 
        675                 680                 685             


Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 


<210>  584
<211>  698
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  584

Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu 
1               5                   10                  15      


Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        595                 600                 605             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    610                 615                 620                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
625                 630                 635                 640 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                645                 650                 655     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly 
            660                 665                 670         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        675                 680                 685             


Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695             


<210>  585
<211>  697
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  585

Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu 
1               5                   10                  15      


Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr 
            20                  25                  30          


Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr 
        35                  40                  45              


Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His 
    50                  55                  60                  


Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly 
65                  70                  75                  80  


Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp 
                85                  90                  95      


Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser 
            100                 105                 110         


Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr 
        115                 120                 125             


Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile 
    130                 135                 140                 


Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala 
145                 150                 155                 160 


Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile 
                165                 170                 175     


Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys 
            180                 185                 190         


Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr 
        195                 200                 205             


Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala 
    210                 215                 220                 


Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser 
225                 230                 235                 240 


Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp 
                245                 250                 255     


Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp 
            260                 265                 270         


Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg 
        275                 280                 285             


Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr 
    290                 295                 300                 


Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln 
305                 310                 315                 320 


Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln 
                325                 330                 335     


His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn 
            340                 345                 350         


Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr 
        355                 360                 365             


Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly 
    370                 375                 380                 


Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp 
385                 390                 395                 400 


Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala 
                405                 410                 415     


Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val 
            420                 425                 430         


Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly 
        435                 440                 445             


Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala 
    450                 455                 460                 


Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys 
465                 470                 475                 480 


Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu 
                485                 490                 495     


Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His 
            500                 505                 510         


Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys 
        515                 520                 525             


Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val 
    530                 535                 540                 


Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn 
545                 550                 555                 560 


Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile 
                565                 570                 575     


Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser 
            580                 585                 590         


Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys 
        595                 600                 605             


Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 
    610                 615                 620                 


Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala 
625                 630                 635                 640 


Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu 
                645                 650                 655     


Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser 
            660                 665                 670         


Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser 
        675                 680                 685             


Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695         


<210>  586
<211>  696
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  586

Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr 
1               5                   10                  15      


Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met 
            20                  25                  30          


Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr 
        35                  40                  45              


Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met 
    50                  55                  60                  


Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val 
65                  70                  75                  80  


Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val 
                85                  90                  95      


Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg 
            100                 105                 110         


Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg 
        115                 120                 125             


Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg 
    130                 135                 140                 


Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala 
145                 150                 155                 160 


Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr 
                165                 170                 175     


Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile 
            180                 185                 190         


Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp 
        195                 200                 205             


Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln 
    210                 215                 220                 


Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn 
225                 230                 235                 240 


Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met 
                245                 250                 255     


Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys 
            260                 265                 270         


Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly 
        275                 280                 285             


Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys 
    290                 295                 300                 


Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro 
305                 310                 315                 320 


Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His 
                325                 330                 335     


Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg 
            340                 345                 350         


Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
        355                 360                 365             


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 
    370                 375                 380                 


Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 
385                 390                 395                 400 


Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 
                405                 410                 415     


Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu 
            420                 425                 430         


Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 
        435                 440                 445             


Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 
    450                 455                 460                 


Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg 
465                 470                 475                 480 


Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 
                485                 490                 495     


Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly 
            500                 505                 510         


Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 
        515                 520                 525             


Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 
    530                 535                 540                 


Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 
545                 550                 555                 560 


Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 
                565                 570                 575     


Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly 
            580                 585                 590         


Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 
        595                 600                 605             


Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly 
    610                 615                 620                 


Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala 
625                 630                 635                 640 


Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile 
                645                 650                 655     


Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile 
            660                 665                 670         


Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr 
        675                 680                 685             


Ala Val Ser Ala Asp Val Gly Cys 
    690                 695     


<210>  587
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  587

Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr 
1               5                   10                  15      


Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr 
            20                  25                  30          


Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu 
        35                  40                  45              


Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys 
    50                  55                  60                  


Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu 
65                  70                  75                  80  


Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val 
                85                  90                  95      


Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
            100                 105                 110         


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
        115                 120                 125             


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
    130                 135                 140                 


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
145                 150                 155                 160 


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
                165                 170                 175     


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
            180                 185                 190         


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
        195                 200                 205             


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
    210                 215                 220                 


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
225                 230                 235                 240 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
                245                 250                 255     


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
            260                 265                 270         


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
        275                 280                 285             


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
    290                 295                 300                 


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
305                 310                 315                 320 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
                325                 330                 335     


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
            340                 345                 350         


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
        515                 520                 525             


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
    610                 615                 620                 


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala Asp Val Gly Cys 
    690                 695 


<210>  588
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  588

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              


Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala Asp Val Gly Cys 
    690                 


<210>  589
<211>  693
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  589

Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met 
1               5                   10                  15      


Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp 
            20                  25                  30          


Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met 
        35                  40                  45              


Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala 
    50                  55                  60                  


Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp 
65                  70                  75                  80  


Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly 
                85                  90                  95      


Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val 
            100                 105                 110         


Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr 
        115                 120                 125             


Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn 
    130                 135                 140                 


Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala 
145                 150                 155                 160 


Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met 
                165                 170                 175     


Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser 
            180                 185                 190         


Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile 
        195                 200                 205             


Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro 
    210                 215                 220                 


Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu 
225                 230                 235                 240 


Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp 
                245                 250                 255     


Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp 
            260                 265                 270         


Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn 
        275                 280                 285             


Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe 
    290                 295                 300                 


Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu 
305                 310                 315                 320 


Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met 
                325                 330                 335     


Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val 
            340                 345                 350         


Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe 
        355                 360                 365             


Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser 
    370                 375                 380                 


Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys 
385                 390                 395                 400 


Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr 
                405                 410                 415     


Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp 
            420                 425                 430         


Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly 
        435                 440                 445             


Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly 
    450                 455                 460                 


Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met 
465                 470                 475                 480 


Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala 
                485                 490                 495     


Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr 
            500                 505                 510         


Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala 
        515                 520                 525             


Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile 
    530                 535                 540                 


Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met 
545                 550                 555                 560 


Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val 
                565                 570                 575     


Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile 
            580                 585                 590         


Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val 
        595                 600                 605             


Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn 
    610                 615                 620                 


Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser 
625                 630                 635                 640 


Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu 
                645                 650                 655     


Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met 
            660                 665                 670         


Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser 
        675                 680                 685             


Ala Asp Val Gly Cys 
    690             


<210>  590
<211>  692
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  590

Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
1               5                   10                  15      


Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
    610                 615                 620                 


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
                645                 650                 655     


Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
            660                 665                 670         


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685             


Asp Val Gly Cys 
    690         


<210>  591
<211>  691
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  591

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
        675                 680                 685             


Val Gly Cys 
    690     


<210>  592
<211>  691
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  592

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Ala Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
        675                 680                 685             


Val Gly Cys 
    690     


<210>  593
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  593

Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr 
1               5                   10                  15      


Ala Met Ala Ala Ala Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr 
            20                  25                  30          


Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu 
        35                  40                  45              


Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys 
    50                  55                  60                  


Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu 
65                  70                  75                  80  


Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val 
                85                  90                  95      


Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
            100                 105                 110         


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
        115                 120                 125             


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
    130                 135                 140                 


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
145                 150                 155                 160 


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
                165                 170                 175     


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
            180                 185                 190         


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
        195                 200                 205             


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
    210                 215                 220                 


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
225                 230                 235                 240 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
                245                 250                 255     


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
            260                 265                 270         


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
        275                 280                 285             


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
    290                 295                 300                 


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
305                 310                 315                 320 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
                325                 330                 335     


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
            340                 345                 350         


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
        515                 520                 525             


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
    610                 615                 620                 


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala Asp Val Gly Cys 
    690                 695 


<210>  594
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  594

Arg Gly Leu Ser Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val 
1               5                   10                  15      


Gln Phe Leu Ile Leu Gly Met Leu Leu Met Thr Gly Gly Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 700             


<210>  595
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  595

Arg Gly Leu Ser Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val 
1               5                   10                  15      


Gln Phe Leu Ile Leu Gly Met Leu Leu Met Thr Gly Ala Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys 
    690                 695                 700             


<210>  596
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  596

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 700             


<210>  597
<211>  702
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  597

Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser 
1               5                   10                  15      


Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val 
            20                  25                  30          


Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala 
        35                  40                  45              


Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr 
    50                  55                  60                  


Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr 
65                  70                  75                  80  


Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys 
                85                  90                  95      


Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His 
            100                 105                 110         


Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser 
        115                 120                 125             


His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser 
    130                 135                 140                 


Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg 
145                 150                 155                 160 


Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly 
                165                 170                 175     


Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile 
            180                 185                 190         


Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe 
        195                 200                 205             


Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His 
    210                 215                 220                 


Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile 
225                 230                 235                 240 


Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr 
                245                 250                 255     


Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro 
            260                 265                 270         


Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val 
        275                 280                 285             


Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu 
    290                 295                 300                 


Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys 
305                 310                 315                 320 


Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile 
                325                 330                 335     


Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp 
            340                 345                 350         


Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro 
        355                 360                 365             


Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly 
    370                 375                 380                 


Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr 
385                 390                 395                 400 


Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His 
                405                 410                 415     


Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His 
            420                 425                 430         


Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys 
        435                 440                 445             


Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr 
    450                 455                 460                 


Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg 
465                 470                 475                 480 


Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg 
                485                 490                 495     


Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr 
            500                 505                 510         


Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln 
        515                 520                 525             


Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val 
    530                 535                 540                 


Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro 
545                 550                 555                 560 


Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp 
                565                 570                 575     


Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys 
            580                 585                 590         


Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe 
        595                 600                 605             


Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr 
    610                 615                 620                 


Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys 
625                 630                 635                 640 


Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly 
                645                 650                 655     


Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu 
            660                 665                 670         


Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu 
        675                 680                 685             


Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 700         


<210>  598
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  598

Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val 
1               5                   10                  15      


Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr 
            20                  25                  30          


Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly 
        35                  40                  45              


Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile 
    50                  55                  60                  


Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu 
65                  70                  75                  80  


Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp 
                85                  90                  95      


Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys 
            100                 105                 110         


Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His 
        115                 120                 125             


Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg 
    130                 135                 140                 


Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn 
145                 150                 155                 160 


Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser 
                165                 170                 175     


Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala 
            180                 185                 190         


Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val 
        195                 200                 205             


Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly 
    210                 215                 220                 


Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu 
225                 230                 235                 240 


Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys 
                245                 250                 255     


Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr 
            260                 265                 270         


Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys 
        275                 280                 285             


Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe 
    290                 295                 300                 


Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys 
305                 310                 315                 320 


Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met 
                325                 330                 335     


Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr 
            340                 345                 350         


Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn 
        355                 360                 365             


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
    370                 375                 380                 


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
385                 390                 395                 400 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
                405                 410                 415     


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
            420                 425                 430         


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
        435                 440                 445             


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
    450                 455                 460                 


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
465                 470                 475                 480 


Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
                485                 490                 495     


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
            500                 505                 510         


Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
        515                 520                 525             


Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp 
    530                 535                 540                 


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
545                 550                 555                 560 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
                565                 570                 575     


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile 
            580                 585                 590         


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
        595                 600                 605             


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
    610                 615                 620                 


Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly 
625                 630                 635                 640 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
                645                 650                 655     


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly 
            660                 665                 670         


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly 
        675                 680                 685             


Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 700     


<210>  599
<211>  700
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  599

Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly 
1               5                   10                  15      


Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 700 


<210>  600
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  600

Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
1               5                   10                  15      


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
            20                  25                  30          


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
        35                  40                  45              


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
    50                  55                  60                  


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
65                  70                  75                  80  


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
                85                  90                  95      


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
            100                 105                 110         


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
        115                 120                 125             


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
    130                 135                 140                 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
145                 150                 155                 160 


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
                165                 170                 175     


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
            180                 185                 190         


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
        195                 200                 205             


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
    210                 215                 220                 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
225                 230                 235                 240 


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
                245                 250                 255     


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
            260                 265                 270         


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
        275                 280                 285             


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
    290                 295                 300                 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
305                 310                 315                 320 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
                325                 330                 335     


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
            340                 345                 350         


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
        355                 360                 365             


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
    370                 375                 380                 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
385                 390                 395                 400 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
                405                 410                 415     


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
            420                 425                 430         


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
        435                 440                 445             


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
    450                 455                 460                 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
465                 470                 475                 480 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
                485                 490                 495     


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
            500                 505                 510         


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
        515                 520                 525             


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
    530                 535                 540                 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
545                 550                 555                 560 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
                565                 570                 575     


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
            580                 585                 590         


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
        595                 600                 605             


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
    610                 615                 620                 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
625                 630                 635                 640 


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
                645                 650                 655     


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
            660                 665                 670         


Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
        675                 680                 685             


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 


<210>  601
<211>  698
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  601

Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val 
1               5                   10                  15      


Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly 
            20                  25                  30          


Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile 
        35                  40                  45              


Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met 
    50                  55                  60                  


Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met 
65                  70                  75                  80  


Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr 
                85                  90                  95      


Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu 
            100                 105                 110         


Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg 
        115                 120                 125             


Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr 
    130                 135                 140                 


Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe 
145                 150                 155                 160 


Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser 
                165                 170                 175     


Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr 
            180                 185                 190         


Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met 
        195                 200                 205             


Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val 
    210                 215                 220                 


Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr 
225                 230                 235                 240 


Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala 
                245                 250                 255     


Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu 
            260                 265                 270         


Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr 
        275                 280                 285             


Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly 
    290                 295                 300                 


Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly 
305                 310                 315                 320 


Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val 
                325                 330                 335     


His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu 
            340                 345                 350         


Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg 
        355                 360                 365             


Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu 
    370                 375                 380                 


Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn 
385                 390                 395                 400 


Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu 
                405                 410                 415     


Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys 
            420                 425                 430         


Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val 
        435                 440                 445             


Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly 
    450                 455                 460                 


Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly 
465                 470                 475                 480 


His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val 
                485                 490                 495     


Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala 
            500                 505                 510         


Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr 
        515                 520                 525             


Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr 
    530                 535                 540                 


Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu 
545                 550                 555                 560 


Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly 
                565                 570                 575     


Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His 
            580                 585                 590         


Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val 
        595                 600                 605             


Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe 
    610                 615                 620                 


Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln 
625                 630                 635                 640 


Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe 
                645                 650                 655     


Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr 
            660                 665                 670         


Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu 
        675                 680                 685             


Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695             


<210>  602
<211>  697
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  602

Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly 
1               5                   10                  15      


Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser 
            20                  25                  30          


Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser 
        35                  40                  45              


Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp 
    50                  55                  60                  


Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu 
65                  70                  75                  80  


Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr 
                85                  90                  95      


Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala 
            100                 105                 110         


Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys 
        115                 120                 125             


Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys 
    130                 135                 140                 


His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala 
145                 150                 155                 160 


Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln 
                165                 170                 175     


Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
            180                 185                 190         


Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
        195                 200                 205             


Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr 
    210                 215                 220                 


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
225                 230                 235                 240 


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
                245                 250                 255     


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
            260                 265                 270         


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
        275                 280                 285             


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
    290                 295                 300                 


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
305                 310                 315                 320 


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
                325                 330                 335     


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
            340                 345                 350         


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
        355                 360                 365             


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
    370                 375                 380                 


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
385                 390                 395                 400 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
                405                 410                 415     


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
            420                 425                 430         


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
        435                 440                 445             


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
    450                 455                 460                 


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
465                 470                 475                 480 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
                485                 490                 495     


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
            500                 505                 510         


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
        515                 520                 525             


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
    530                 535                 540                 


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
545                 550                 555                 560 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
                565                 570                 575     


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
            580                 585                 590         


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
        595                 600                 605             


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
    610                 615                 620                 


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
625                 630                 635                 640 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
                645                 650                 655     


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
            660                 665                 670         


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
        675                 680                 685             


Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695         


<210>  603
<211>  696
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  603

Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu 
1               5                   10                  15      


Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
        595                 600                 605             


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe 
625                 630                 635                 640 


Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln 
                645                 650                 655     


Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn 
            660                 665                 670         


Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe 
        675                 680                 685             


Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695     


<210>  604
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  604

Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu 
1               5                   10                  15      


Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        595                 600                 605             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    610                 615                 620                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
625                 630                 635                 640 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                645                 650                 655     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly 
            660                 665                 670         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        675                 680                 685             


Ser Thr Ala Val Ser Ala Asp 
    690                 695 


<210>  605
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  605

Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu 
1               5                   10                  15      


Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr 
            20                  25                  30          


Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr 
        35                  40                  45              


Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His 
    50                  55                  60                  


Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly 
65                  70                  75                  80  


Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp 
                85                  90                  95      


Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser 
            100                 105                 110         


Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr 
        115                 120                 125             


Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile 
    130                 135                 140                 


Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala 
145                 150                 155                 160 


Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile 
                165                 170                 175     


Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys 
            180                 185                 190         


Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr 
        195                 200                 205             


Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala 
    210                 215                 220                 


Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser 
225                 230                 235                 240 


Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp 
                245                 250                 255     


Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp 
            260                 265                 270         


Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg 
        275                 280                 285             


Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr 
    290                 295                 300                 


Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln 
305                 310                 315                 320 


Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln 
                325                 330                 335     


His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn 
            340                 345                 350         


Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr 
        355                 360                 365             


Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly 
    370                 375                 380                 


Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp 
385                 390                 395                 400 


Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala 
                405                 410                 415     


Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val 
            420                 425                 430         


Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly 
        435                 440                 445             


Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala 
    450                 455                 460                 


Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys 
465                 470                 475                 480 


Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu 
                485                 490                 495     


Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His 
            500                 505                 510         


Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys 
        515                 520                 525             


Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val 
    530                 535                 540                 


Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn 
545                 550                 555                 560 


Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile 
                565                 570                 575     


Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser 
            580                 585                 590         


Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys 
        595                 600                 605             


Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 
    610                 615                 620                 


Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala 
625                 630                 635                 640 


Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu 
                645                 650                 655     


Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser 
            660                 665                 670         


Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser 
        675                 680                 685             


Thr Ala Val Ser Ala Asp 
    690                 


<210>  606
<211>  693
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  606

Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr 
1               5                   10                  15      


Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met 
            20                  25                  30          


Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr 
        35                  40                  45              


Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met 
    50                  55                  60                  


Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val 
65                  70                  75                  80  


Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val 
                85                  90                  95      


Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg 
            100                 105                 110         


Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg 
        115                 120                 125             


Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg 
    130                 135                 140                 


Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala 
145                 150                 155                 160 


Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr 
                165                 170                 175     


Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile 
            180                 185                 190         


Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp 
        195                 200                 205             


Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln 
    210                 215                 220                 


Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn 
225                 230                 235                 240 


Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met 
                245                 250                 255     


Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys 
            260                 265                 270         


Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly 
        275                 280                 285             


Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys 
    290                 295                 300                 


Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro 
305                 310                 315                 320 


Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His 
                325                 330                 335     


Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg 
            340                 345                 350         


Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
        355                 360                 365             


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 
    370                 375                 380                 


Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 
385                 390                 395                 400 


Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 
                405                 410                 415     


Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu 
            420                 425                 430         


Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 
        435                 440                 445             


Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 
    450                 455                 460                 


Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg 
465                 470                 475                 480 


Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 
                485                 490                 495     


Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly 
            500                 505                 510         


Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 
        515                 520                 525             


Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 
    530                 535                 540                 


Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 
545                 550                 555                 560 


Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 
                565                 570                 575     


Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly 
            580                 585                 590         


Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 
        595                 600                 605             


Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly 
    610                 615                 620                 


Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala 
625                 630                 635                 640 


Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile 
                645                 650                 655     


Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile 
            660                 665                 670         


Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr 
        675                 680                 685             


Ala Val Ser Ala Asp 
    690             


<210>  607
<211>  692
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  607

Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr 
1               5                   10                  15      


Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr 
            20                  25                  30          


Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu 
        35                  40                  45              


Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys 
    50                  55                  60                  


Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu 
65                  70                  75                  80  


Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val 
                85                  90                  95      


Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
            100                 105                 110         


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
        115                 120                 125             


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
    130                 135                 140                 


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
145                 150                 155                 160 


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
                165                 170                 175     


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
            180                 185                 190         


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
        195                 200                 205             


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
    210                 215                 220                 


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
225                 230                 235                 240 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
                245                 250                 255     


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
            260                 265                 270         


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
        275                 280                 285             


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
    290                 295                 300                 


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
305                 310                 315                 320 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
                325                 330                 335     


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
            340                 345                 350         


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
        515                 520                 525             


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
    610                 615                 620                 


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala Asp 
    690         


<210>  608
<211>  691
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  608

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              


Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala Asp 
    690     


<210>  609
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  609

Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met 
1               5                   10                  15      


Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp 
            20                  25                  30          


Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met 
        35                  40                  45              


Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala 
    50                  55                  60                  


Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp 
65                  70                  75                  80  


Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly 
                85                  90                  95      


Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val 
            100                 105                 110         


Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr 
        115                 120                 125             


Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn 
    130                 135                 140                 


Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala 
145                 150                 155                 160 


Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met 
                165                 170                 175     


Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser 
            180                 185                 190         


Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile 
        195                 200                 205             


Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro 
    210                 215                 220                 


Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu 
225                 230                 235                 240 


Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp 
                245                 250                 255     


Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp 
            260                 265                 270         


Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn 
        275                 280                 285             


Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe 
    290                 295                 300                 


Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu 
305                 310                 315                 320 


Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met 
                325                 330                 335     


Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val 
            340                 345                 350         


Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe 
        355                 360                 365             


Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser 
    370                 375                 380                 


Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys 
385                 390                 395                 400 


Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr 
                405                 410                 415     


Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp 
            420                 425                 430         


Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly 
        435                 440                 445             


Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly 
    450                 455                 460                 


Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met 
465                 470                 475                 480 


Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala 
                485                 490                 495     


Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr 
            500                 505                 510         


Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala 
        515                 520                 525             


Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile 
    530                 535                 540                 


Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met 
545                 550                 555                 560 


Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val 
                565                 570                 575     


Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile 
            580                 585                 590         


Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val 
        595                 600                 605             


Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn 
    610                 615                 620                 


Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser 
625                 630                 635                 640 


Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu 
                645                 650                 655     


Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met 
            660                 665                 670         


Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser 
        675                 680                 685             


Ala Asp 
    690 


<210>  610
<211>  689
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  610

Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
1               5                   10                  15      


Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
    610                 615                 620                 


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
                645                 650                 655     


Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
            660                 665                 670         


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685             


Asp 
    


<210>  611
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  611

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
        675                 680                 685             


<210>  612
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  612

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Ala Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
        675                 680                 685             


<210>  613
<211>  692
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  613

Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr 
1               5                   10                  15      


Ala Met Ala Ala Ala Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr 
            20                  25                  30          


Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu 
        35                  40                  45              


Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys 
    50                  55                  60                  


Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu 
65                  70                  75                  80  


Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val 
                85                  90                  95      


Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
            100                 105                 110         


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
        115                 120                 125             


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
    130                 135                 140                 


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
145                 150                 155                 160 


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
                165                 170                 175     


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
            180                 185                 190         


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
        195                 200                 205             


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
    210                 215                 220                 


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
225                 230                 235                 240 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
                245                 250                 255     


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
            260                 265                 270         


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
        275                 280                 285             


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
    290                 295                 300                 


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
305                 310                 315                 320 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
                325                 330                 335     


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
            340                 345                 350         


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
        515                 520                 525             


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
    610                 615                 620                 


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala Asp 
    690         


<210>  614
<211>  700
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  614

Arg Gly Leu Ser Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val 
1               5                   10                  15      


Gln Phe Leu Ile Leu Gly Met Leu Leu Met Thr Gly Gly Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 700 


<210>  615
<211>  700
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  615

Arg Gly Leu Ser Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val 
1               5                   10                  15      


Gln Phe Leu Ile Leu Gly Met Leu Leu Met Thr Gly Ala Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp 
    690                 695                 700 


<210>  616
<211>  702
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  616

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695                 700         


<210>  617
<211>  700
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  617

Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val 
1               5                   10                  15      


Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr 
            20                  25                  30          


Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly 
        35                  40                  45              


Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile 
    50                  55                  60                  


Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu 
65                  70                  75                  80  


Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp 
                85                  90                  95      


Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys 
            100                 105                 110         


Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His 
        115                 120                 125             


Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg 
    130                 135                 140                 


Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn 
145                 150                 155                 160 


Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser 
                165                 170                 175     


Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala 
            180                 185                 190         


Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val 
        195                 200                 205             


Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly 
    210                 215                 220                 


Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu 
225                 230                 235                 240 


Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys 
                245                 250                 255     


Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr 
            260                 265                 270         


Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys 
        275                 280                 285             


Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe 
    290                 295                 300                 


Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys 
305                 310                 315                 320 


Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met 
                325                 330                 335     


Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr 
            340                 345                 350         


Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn 
        355                 360                 365             


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
    370                 375                 380                 


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
385                 390                 395                 400 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
                405                 410                 415     


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
            420                 425                 430         


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
        435                 440                 445             


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
    450                 455                 460                 


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
465                 470                 475                 480 


Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
                485                 490                 495     


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
            500                 505                 510         


Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
        515                 520                 525             


Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp 
    530                 535                 540                 


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
545                 550                 555                 560 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
                565                 570                 575     


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile 
            580                 585                 590         


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
        595                 600                 605             


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
    610                 615                 620                 


Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly 
625                 630                 635                 640 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
                645                 650                 655     


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly 
            660                 665                 670         


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly 
        675                 680                 685             


Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695                 700 


<210>  618
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  618

Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser 
1               5                   10                  15      


Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val 
            20                  25                  30          


Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala 
        35                  40                  45              


Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr 
    50                  55                  60                  


Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr 
65                  70                  75                  80  


Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys 
                85                  90                  95      


Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His 
            100                 105                 110         


Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser 
        115                 120                 125             


His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser 
    130                 135                 140                 


Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg 
145                 150                 155                 160 


Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly 
                165                 170                 175     


Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile 
            180                 185                 190         


Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe 
        195                 200                 205             


Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His 
    210                 215                 220                 


Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile 
225                 230                 235                 240 


Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr 
                245                 250                 255     


Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro 
            260                 265                 270         


Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val 
        275                 280                 285             


Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu 
    290                 295                 300                 


Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys 
305                 310                 315                 320 


Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile 
                325                 330                 335     


Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp 
            340                 345                 350         


Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro 
        355                 360                 365             


Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly 
    370                 375                 380                 


Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr 
385                 390                 395                 400 


Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His 
                405                 410                 415     


Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His 
            420                 425                 430         


Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys 
        435                 440                 445             


Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr 
    450                 455                 460                 


Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg 
465                 470                 475                 480 


Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg 
                485                 490                 495     


Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr 
            500                 505                 510         


Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln 
        515                 520                 525             


Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val 
    530                 535                 540                 


Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro 
545                 550                 555                 560 


Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp 
                565                 570                 575     


Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys 
            580                 585                 590         


Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe 
        595                 600                 605             


Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr 
    610                 615                 620                 


Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys 
625                 630                 635                 640 


Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly 
                645                 650                 655     


Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu 
            660                 665                 670         


Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu 
        675                 680                 685             


Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695                 700     


<210>  619
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  619

Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly 
1               5                   10                  15      


Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695                 


<210>  620
<211>  698
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  620

Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
1               5                   10                  15      


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
            20                  25                  30          


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
        35                  40                  45              


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
    50                  55                  60                  


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
65                  70                  75                  80  


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
                85                  90                  95      


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
            100                 105                 110         


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
        115                 120                 125             


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
    130                 135                 140                 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
145                 150                 155                 160 


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
                165                 170                 175     


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
            180                 185                 190         


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
        195                 200                 205             


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
    210                 215                 220                 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
225                 230                 235                 240 


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
                245                 250                 255     


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
            260                 265                 270         


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
        275                 280                 285             


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
    290                 295                 300                 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
305                 310                 315                 320 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
                325                 330                 335     


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
            340                 345                 350         


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
        355                 360                 365             


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
    370                 375                 380                 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
385                 390                 395                 400 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
                405                 410                 415     


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
            420                 425                 430         


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
        435                 440                 445             


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
    450                 455                 460                 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
465                 470                 475                 480 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
                485                 490                 495     


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
            500                 505                 510         


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
        515                 520                 525             


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
    530                 535                 540                 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
545                 550                 555                 560 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
                565                 570                 575     


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
            580                 585                 590         


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
        595                 600                 605             


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
    610                 615                 620                 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
625                 630                 635                 640 


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
                645                 650                 655     


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
            660                 665                 670         


Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
        675                 680                 685             


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695             


<210>  621
<211>  697
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  621

Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val 
1               5                   10                  15      


Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly 
            20                  25                  30          


Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile 
        35                  40                  45              


Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met 
    50                  55                  60                  


Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met 
65                  70                  75                  80  


Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr 
                85                  90                  95      


Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu 
            100                 105                 110         


Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg 
        115                 120                 125             


Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr 
    130                 135                 140                 


Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe 
145                 150                 155                 160 


Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser 
                165                 170                 175     


Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr 
            180                 185                 190         


Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met 
        195                 200                 205             


Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val 
    210                 215                 220                 


Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr 
225                 230                 235                 240 


Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala 
                245                 250                 255     


Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu 
            260                 265                 270         


Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr 
        275                 280                 285             


Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly 
    290                 295                 300                 


Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly 
305                 310                 315                 320 


Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val 
                325                 330                 335     


His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu 
            340                 345                 350         


Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg 
        355                 360                 365             


Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu 
    370                 375                 380                 


Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn 
385                 390                 395                 400 


Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu 
                405                 410                 415     


Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys 
            420                 425                 430         


Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val 
        435                 440                 445             


Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly 
    450                 455                 460                 


Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly 
465                 470                 475                 480 


His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val 
                485                 490                 495     


Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala 
            500                 505                 510         


Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr 
        515                 520                 525             


Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr 
    530                 535                 540                 


Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu 
545                 550                 555                 560 


Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly 
                565                 570                 575     


Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His 
            580                 585                 590         


Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val 
        595                 600                 605             


Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe 
    610                 615                 620                 


Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln 
625                 630                 635                 640 


Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe 
                645                 650                 655     


Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr 
            660                 665                 670         


Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu 
        675                 680                 685             


Ile Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695         


<210>  622
<211>  696
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  622

Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly 
1               5                   10                  15      


Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser 
            20                  25                  30          


Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser 
        35                  40                  45              


Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp 
    50                  55                  60                  


Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu 
65                  70                  75                  80  


Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr 
                85                  90                  95      


Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala 
            100                 105                 110         


Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys 
        115                 120                 125             


Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys 
    130                 135                 140                 


His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala 
145                 150                 155                 160 


Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln 
                165                 170                 175     


Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
            180                 185                 190         


Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
        195                 200                 205             


Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr 
    210                 215                 220                 


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
225                 230                 235                 240 


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
                245                 250                 255     


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
            260                 265                 270         


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
        275                 280                 285             


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
    290                 295                 300                 


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
305                 310                 315                 320 


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
                325                 330                 335     


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
            340                 345                 350         


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
        355                 360                 365             


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
    370                 375                 380                 


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
385                 390                 395                 400 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
                405                 410                 415     


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
            420                 425                 430         


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
        435                 440                 445             


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
    450                 455                 460                 


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
465                 470                 475                 480 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
                485                 490                 495     


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
            500                 505                 510         


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
        515                 520                 525             


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
    530                 535                 540                 


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
545                 550                 555                 560 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
                565                 570                 575     


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
            580                 585                 590         


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
        595                 600                 605             


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
    610                 615                 620                 


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
625                 630                 635                 640 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
                645                 650                 655     


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
            660                 665                 670         


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
        675                 680                 685             


Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695     


<210>  623
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  623

Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu 
1               5                   10                  15      


Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
        595                 600                 605             


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe 
625                 630                 635                 640 


Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln 
                645                 650                 655     


Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn 
            660                 665                 670         


Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe 
        675                 680                 685             


Leu Ser Thr Ala Val Ser Ala 
    690                 695 


<210>  624
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  624

Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu 
1               5                   10                  15      


Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        595                 600                 605             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    610                 615                 620                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
625                 630                 635                 640 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                645                 650                 655     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly 
            660                 665                 670         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        675                 680                 685             


Ser Thr Ala Val Ser Ala 
    690                 


<210>  625
<211>  693
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  625

Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu 
1               5                   10                  15      


Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr 
            20                  25                  30          


Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr 
        35                  40                  45              


Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His 
    50                  55                  60                  


Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly 
65                  70                  75                  80  


Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp 
                85                  90                  95      


Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser 
            100                 105                 110         


Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr 
        115                 120                 125             


Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile 
    130                 135                 140                 


Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala 
145                 150                 155                 160 


Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile 
                165                 170                 175     


Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys 
            180                 185                 190         


Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr 
        195                 200                 205             


Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala 
    210                 215                 220                 


Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser 
225                 230                 235                 240 


Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp 
                245                 250                 255     


Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp 
            260                 265                 270         


Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg 
        275                 280                 285             


Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr 
    290                 295                 300                 


Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln 
305                 310                 315                 320 


Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln 
                325                 330                 335     


His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn 
            340                 345                 350         


Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr 
        355                 360                 365             


Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly 
    370                 375                 380                 


Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp 
385                 390                 395                 400 


Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala 
                405                 410                 415     


Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val 
            420                 425                 430         


Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly 
        435                 440                 445             


Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala 
    450                 455                 460                 


Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys 
465                 470                 475                 480 


Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu 
                485                 490                 495     


Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His 
            500                 505                 510         


Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys 
        515                 520                 525             


Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val 
    530                 535                 540                 


Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn 
545                 550                 555                 560 


Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile 
                565                 570                 575     


Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser 
            580                 585                 590         


Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys 
        595                 600                 605             


Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 
    610                 615                 620                 


Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala 
625                 630                 635                 640 


Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu 
                645                 650                 655     


Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser 
            660                 665                 670         


Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser 
        675                 680                 685             


Thr Ala Val Ser Ala 
    690             


<210>  626
<211>  692
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  626

Arg Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr 
1               5                   10                  15      


Thr Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met 
            20                  25                  30          


Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr 
        35                  40                  45              


Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met 
    50                  55                  60                  


Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val 
65                  70                  75                  80  


Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val 
                85                  90                  95      


Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg 
            100                 105                 110         


Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg 
        115                 120                 125             


Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg 
    130                 135                 140                 


Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala 
145                 150                 155                 160 


Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr 
                165                 170                 175     


Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile 
            180                 185                 190         


Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp 
        195                 200                 205             


Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln 
    210                 215                 220                 


Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn 
225                 230                 235                 240 


Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met 
                245                 250                 255     


Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys 
            260                 265                 270         


Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly 
        275                 280                 285             


Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys 
    290                 295                 300                 


Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro 
305                 310                 315                 320 


Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His 
                325                 330                 335     


Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg 
            340                 345                 350         


Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
        355                 360                 365             


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 
    370                 375                 380                 


Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 
385                 390                 395                 400 


Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 
                405                 410                 415     


Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu 
            420                 425                 430         


Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 
        435                 440                 445             


Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 
    450                 455                 460                 


Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg 
465                 470                 475                 480 


Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 
                485                 490                 495     


Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly 
            500                 505                 510         


Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 
        515                 520                 525             


Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 
    530                 535                 540                 


Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 
545                 550                 555                 560 


Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 
                565                 570                 575     


Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly 
            580                 585                 590         


Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 
        595                 600                 605             


Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly 
    610                 615                 620                 


Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala 
625                 630                 635                 640 


Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile 
                645                 650                 655     


Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile 
            660                 665                 670         


Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr 
        675                 680                 685             


Ala Val Ser Ala 
    690         


<210>  627
<211>  691
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  627

Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr 
1               5                   10                  15      


Ala Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr 
            20                  25                  30          


Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu 
        35                  40                  45              


Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys 
    50                  55                  60                  


Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu 
65                  70                  75                  80  


Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val 
                85                  90                  95      


Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
            100                 105                 110         


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
        115                 120                 125             


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
    130                 135                 140                 


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
145                 150                 155                 160 


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
                165                 170                 175     


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
            180                 185                 190         


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
        195                 200                 205             


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
    210                 215                 220                 


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
225                 230                 235                 240 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
                245                 250                 255     


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
            260                 265                 270         


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
        275                 280                 285             


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
    290                 295                 300                 


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
305                 310                 315                 320 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
                325                 330                 335     


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
            340                 345                 350         


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
        515                 520                 525             


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
    610                 615                 620                 


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala 
    690     


<210>  628
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  628

Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              


Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala 
    690 


<210>  629
<211>  689
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  629

Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met 
1               5                   10                  15      


Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp 
            20                  25                  30          


Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met 
        35                  40                  45              


Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala 
    50                  55                  60                  


Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp 
65                  70                  75                  80  


Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly 
                85                  90                  95      


Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val 
            100                 105                 110         


Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr 
        115                 120                 125             


Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn 
    130                 135                 140                 


Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala 
145                 150                 155                 160 


Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met 
                165                 170                 175     


Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser 
            180                 185                 190         


Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile 
        195                 200                 205             


Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro 
    210                 215                 220                 


Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu 
225                 230                 235                 240 


Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp 
                245                 250                 255     


Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp 
            260                 265                 270         


Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn 
        275                 280                 285             


Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe 
    290                 295                 300                 


Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu 
305                 310                 315                 320 


Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met 
                325                 330                 335     


Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val 
            340                 345                 350         


Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe 
        355                 360                 365             


Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser 
    370                 375                 380                 


Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys 
385                 390                 395                 400 


Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr 
                405                 410                 415     


Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp 
            420                 425                 430         


Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly 
        435                 440                 445             


Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly 
    450                 455                 460                 


Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met 
465                 470                 475                 480 


Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala 
                485                 490                 495     


Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr 
            500                 505                 510         


Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala 
        515                 520                 525             


Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile 
    530                 535                 540                 


Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met 
545                 550                 555                 560 


Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val 
                565                 570                 575     


Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile 
            580                 585                 590         


Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val 
        595                 600                 605             


Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn 
    610                 615                 620                 


Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser 
625                 630                 635                 640 


Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu 
                645                 650                 655     


Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met 
            660                 665                 670         


Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser 
        675                 680                 685             


Ala 
    


<210>  630
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  630

Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
1               5                   10                  15      


Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
    610                 615                 620                 


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
                645                 650                 655     


Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
            660                 665                 670         


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685             


<210>  631
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)

<400>  631

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  632
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  632

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Ala Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  633
<211>  691
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  633

Arg Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr 
1               5                   10                  15      


Ala Met Ala Ala Ala Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr 
            20                  25                  30          


Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu 
        35                  40                  45              


Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys 
    50                  55                  60                  


Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu 
65                  70                  75                  80  


Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val 
                85                  90                  95      


Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg 
            100                 105                 110         


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
        115                 120                 125             


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
    130                 135                 140                 


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
145                 150                 155                 160 


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
                165                 170                 175     


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
            180                 185                 190         


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
        195                 200                 205             


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
    210                 215                 220                 


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
225                 230                 235                 240 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
                245                 250                 255     


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
            260                 265                 270         


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
        275                 280                 285             


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
    290                 295                 300                 


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
305                 310                 315                 320 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
                325                 330                 335     


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
            340                 345                 350         


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
        515                 520                 525             


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
    610                 615                 620                 


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala 
    690     


<210>  634
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  634

Arg Gly Leu Ser Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val 
1               5                   10                  15      


Gln Phe Leu Ile Leu Gly Met Leu Leu Met Thr Gly Gly Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695                 


<210>  635
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant

<400>  635

Arg Gly Leu Ser Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val 
1               5                   10                  15      


Gln Phe Leu Ile Leu Gly Met Leu Leu Met Thr Gly Ala Val Thr Arg 
            20                  25                  30          


Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu 
        35                  40                  45              


Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln 
    50                  55                  60                  


Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys 
65                  70                  75                  80  


Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys 
                85                  90                  95      


Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys 
            100                 105                 110         


Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser 
        115                 120                 125             


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
    130                 135                 140                 


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
145                 150                 155                 160 


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
                165                 170                 175     


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
            180                 185                 190         


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
        195                 200                 205             


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
    210                 215                 220                 


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
225                 230                 235                 240 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
                245                 250                 255     


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
            260                 265                 270         


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
        275                 280                 285             


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
    290                 295                 300                 


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
305                 310                 315                 320 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
                325                 330                 335     


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
            340                 345                 350         


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
        355                 360                 365             


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
    370                 375                 380                 


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
385                 390                 395                 400 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
                405                 410                 415     


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
            420                 425                 430         


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
        435                 440                 445             


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
    450                 455                 460                 


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
465                 470                 475                 480 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
                485                 490                 495     


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
            500                 505                 510         


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
        515                 520                 525             


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
    530                 535                 540                 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
545                 550                 555                 560 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
                565                 570                 575     


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
            580                 585                 590         


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
        595                 600                 605             


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
    610                 615                 620                 


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
625                 630                 635                 640 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
                645                 650                 655     


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
            660                 665                 670         


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
        675                 680                 685             


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
    690                 695                 


<210>  636
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long

<400>  636

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  637
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long

<400>  637

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  638
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long

<400>  638

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr 
            340                 345                 350         


Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
        355                 360                 365             


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
    370                 375                 380                 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
385                 390                 395                 400 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
                405                 410                 415     


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
            420                 425                 430         


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
        435                 440                 445             


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
    450                 455                 460                 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser 
465                 470                 475                 480 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
                485                 490                 495     


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
            500                 505                 510         


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
        515                 520                 525             


Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln 
    530                 535                 540                 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
545                 550                 555                 560 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
                565                 570                 575     


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
            580                 585                 590         


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
        595                 600                 605             


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
    610                 615                 620                 


Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His 
625                 630                 635                 640 


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
                645                 650                 655     


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn 
            660                 665                 670         


Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val 
        675                 680                 685             


Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
    690                 695                 700                 


Asp Phe Ser Lys 
705             


<210>  639
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long

<400>  639

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  640
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long

<400>  640

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly Tyr Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Lys Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  641
<211>  706
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long

<400>  641

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Thr Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 
        355                 360                 365             


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
    370                 375                 380                 


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
385                 390                 395                 400 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
                405                 410                 415     


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
            420                 425                 430         


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
        435                 440                 445             


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
    450                 455                 460                 


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 
465                 470                 475                 480 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
                485                 490                 495     


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu 
            500                 505                 510         


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
        515                 520                 525             


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
    530                 535                 540                 


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
545                 550                 555                 560 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
                565                 570                 575     


Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp 
            580                 585                 590         


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
        595                 600                 605             


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
    610                 615                 620                 


Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
625                 630                 635                 640 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
                645                 650                 655     


Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys 
            660                 665                 670         


Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 
        675                 680                 685             


Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe 
    690                 695                 700                 


Ser Lys 
705     


<210>  642
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long

<400>  642

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


Arg Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly Tyr Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Lys Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  643
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long

<400>  643

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Thr Val Asn 
            340                 345                 350         


Asp Ile Gly Tyr Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Lys Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  644
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long

<400>  644

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr 
            340                 345                 350         


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
        355                 360                 365             


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
    370                 375                 380                 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
385                 390                 395                 400 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
                405                 410                 415     


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
            420                 425                 430         


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
        435                 440                 445             


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
    450                 455                 460                 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser 
465                 470                 475                 480 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
                485                 490                 495     


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
            500                 505                 510         


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
        515                 520                 525             


Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln 
    530                 535                 540                 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
545                 550                 555                 560 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
                565                 570                 575     


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
            580                 585                 590         


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
        595                 600                 605             


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
    610                 615                 620                 


Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His 
625                 630                 635                 640 


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
                645                 650                 655     


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn 
            660                 665                 670         


Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val 
        675                 680                 685             


Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
    690                 695                 700                 


Asp Phe Ser Lys 
705             


<210>  645
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long

<400>  645

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Arg Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  646
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long

<400>  646

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Ala 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  647
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long

<400>  647

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Leu Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  648
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long

<400>  648

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp Tyr Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  649
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long

<400>  649

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Ile Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  650
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long

<400>  650

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Leu Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  651
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long

<400>  651

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Thr Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  652
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long

<400>  652

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Val Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  653
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long

<400>  653

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Ala Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Pro Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  654
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long

<400>  654

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Lys Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  655
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long

<400>  655

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Arg Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  656
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long

<400>  656

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Ser Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Thr Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  657
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long

<400>  657

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Asn Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Arg Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  658
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long

<400>  658

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Glu Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  659
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long

<400>  659

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Leu Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  660
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long

<400>  660

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Asn Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Met Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  661
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long

<400>  661

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Leu 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Ile Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  662
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long

<400>  662

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Ser Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Thr Ile Ser Asp Ile Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Ala Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  663
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long

<400>  663

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Thr Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  664
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long

<400>  664

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Leu 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  665
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long

<400>  665

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Glu Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Ile Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  666
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long

<400>  666

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Ile Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  667
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long

<400>  667

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Val Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Phe Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  668
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long

<400>  668

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Lys Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  669
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long

<400>  669

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Glu Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  670
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long

<400>  670

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Ala Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  671
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long

<400>  671

Arg Ile Ile Asn Ala Gly Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Asn Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Arg Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  672
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long

<400>  672

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Glu Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Lys Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  673
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long

<400>  673

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Val Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  674
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long

<400>  674

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Asn Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Met Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  675
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long

<400>  675

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Ala Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Thr Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  676
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long

<400>  676

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Glu Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Leu Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  677
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long

<400>  677

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Ile Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ile Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  678
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long

<400>  678

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Ile Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  679
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long

<400>  679

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Gln Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  680
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long

<400>  680

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Ala Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  681
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long

<400>  681

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  682
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long

<400>  682

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Thr Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Thr Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Gly Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  683
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long

<400>  683

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Ala Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Leu Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Ala His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Ala Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Val Asp Gly Thr Val Thr Val Glu Gly 
        515                 520                 525             


Gln Tyr Gly Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Ile Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Gly Gly Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  684
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long

<400>  684

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Thr Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Ser Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Glu Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  685
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long

<400>  685

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Thr Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  686
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long

<400>  686

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Thr Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Asn Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Met Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  687
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long

<400>  687

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Thr Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Val Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  688
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long

<400>  688

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Thr Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Ala Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  689
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long

<400>  689

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Val Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  690
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long

<400>  690

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp Tyr Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  691
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long

<400>  691

Arg Ile Ile Asn Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val Glu 
            20                  25                  30          


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Pro Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  692
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long

<400>  692

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Thr Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  693
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long

<400>  693

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Asn Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Val His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  694
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long

<400>  694

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  695
<211>  706
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long

<400>  695

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Asn Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 
        355                 360                 365             


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
    370                 375                 380                 


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
385                 390                 395                 400 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
                405                 410                 415     


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
            420                 425                 430         


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
        435                 440                 445             


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
    450                 455                 460                 


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 
465                 470                 475                 480 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
                485                 490                 495     


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu 
            500                 505                 510         


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
        515                 520                 525             


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
    530                 535                 540                 


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
545                 550                 555                 560 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
                565                 570                 575     


Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp 
            580                 585                 590         


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
        595                 600                 605             


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
    610                 615                 620                 


Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
625                 630                 635                 640 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
                645                 650                 655     


Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys 
            660                 665                 670         


Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 
        675                 680                 685             


Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe 
    690                 695                 700                 


Ser Lys 
705     


<210>  696
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long

<400>  696

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  697
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long

<400>  697

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys Gln 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Val Cys Thr Ala 
            500                 505                 510         


Ala Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  698
<211>  706
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long

<400>  698

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Ala 
1               5                   10                  15      


Ser Ile Gly Ile Val Ser Leu Leu Leu Thr Thr Val Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Val Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 
        355                 360                 365             


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
    370                 375                 380                 


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
385                 390                 395                 400 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
                405                 410                 415     


Trp His Ser Gly Ala Asp Thr Glu Thr Pro His Trp Asn Asn Lys Glu 
            420                 425                 430         


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
        435                 440                 445             


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
    450                 455                 460                 


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
465                 470                 475                 480 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
                485                 490                 495     


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu 
            500                 505                 510         


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Arg Asp 
        515                 520                 525             


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
    530                 535                 540                 


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
545                 550                 555                 560 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
                565                 570                 575     


Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp 
            580                 585                 590         


His Arg Ser Gly Ser Ile Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
        595                 600                 605             


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
    610                 615                 620                 


Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
625                 630                 635                 640 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
                645                 650                 655     


Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys 
            660                 665                 670         


Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 
        675                 680                 685             


Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe 
    690                 695                 700                 


Ser Lys 
705     


<210>  699
<211>  706
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long

<400>  699

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu 
                165                 170                 175     


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 
        355                 360                 365             


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
    370                 375                 380                 


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
385                 390                 395                 400 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
                405                 410                 415     


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
            420                 425                 430         


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
        435                 440                 445             


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
    450                 455                 460                 


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 
465                 470                 475                 480 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
                485                 490                 495     


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu 
            500                 505                 510         


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
        515                 520                 525             


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
    530                 535                 540                 


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
545                 550                 555                 560 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
                565                 570                 575     


Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp 
            580                 585                 590         


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
        595                 600                 605             


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
    610                 615                 620                 


Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
625                 630                 635                 640 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
                645                 650                 655     


Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys 
            660                 665                 670         


Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 
        675                 680                 685             


Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val Asp Phe 
    690                 695                 700                 


Ser Lys 
705     


<210>  700
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long

<400>  700

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Thr Arg Arg Ser Arg Arg Ser Val Ser Leu Arg 
        115                 120                 125             


Tyr His Tyr Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Lys Lys His Leu Ile Met Val Glu Asn Trp Ile Phe 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Ala Ile Val Ser Val Ala Ile Thr Trp Leu Met 
                165                 170                 175     


Gly Ser Leu Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Val Leu 
            180                 185                 190         


Ile Val Pro Ala Tyr Ser Ile Ser Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Leu Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Glu Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Met Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Leu Ser Asp Met Ala Ser Ala Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Pro Ser Leu Asp Lys Gln Ser Asp Thr Gln Ser 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Gly Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Ile Phe Gly Lys Gly Ser Leu Val Thr Cys Ser Lys Phe Thr Cys Cys 
305                 310                 315                 320 


Lys Lys Met Pro Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Pro Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Ser Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe Ser Lys 
705                 710         


<210>  701
<211>  710
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long

<400>  701

Arg Ile Ile Asn Ala Arg Lys Glu Arg Lys Arg Arg Gly Ala Asp Thr 
1               5                   10                  15      


Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
            20                  25                  30          


Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser Asp 
        35                  40                  45              


Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Met Gly Val Asn Lys Cys 
    50                  55                  60                  


His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
65                  70                  75                  80  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
                85                  90                  95      


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
            100                 105                 110         


His Lys Lys Gly Glu Ala Arg Pro Ser Arg Arg Ala Val Thr Leu Pro 
        115                 120                 125             


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
    130                 135                 140                 


Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Leu 
145                 150                 155                 160 


Arg Asn Pro Gly Phe Thr Val Ala Ala Leu Ala Met Ala Trp Leu Leu 
                165                 170                 175     


Gly Asn Ser Thr Ser Gln Arg Val Ile Tyr Leu Val Met Ile Leu Leu 
            180                 185                 190         


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
        195                 200                 205             


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
    210                 215                 220                 


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
225                 230                 235                 240 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
                245                 250                 255     


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
            260                 265                 270         


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
        275                 280                 285             


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
    290                 295                 300                 


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser 
305                 310                 315                 320 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
                325                 330                 335     


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
            340                 345                 350         


Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr 
        355                 360                 365             


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
    370                 375                 380                 


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
385                 390                 395                 400 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
                405                 410                 415     


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
            420                 425                 430         


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
        435                 440                 445             


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
    450                 455                 460                 


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
465                 470                 475                 480 


Arg Leu Phe Ser Gly His Leu Lys Cys Arg Ile Lys Met Asp Lys Leu 
                485                 490                 495     


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
            500                 505                 510         


Ser Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
        515                 520                 525             


Gln Tyr Ala Gly Arg Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
    530                 535                 540                 


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
545                 550                 555                 560 


Pro Ile Ile Thr Glu Ser Thr Glu Asn Ser Arg Met Met Leu Glu Leu 
                565                 570                 575     


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys 
            580                 585                 590         


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
        595                 600                 605             


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
    610                 615                 620                 


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly 
625                 630                 635                 640 


Lys Gly Val His Gln Val Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
                645                 650                 655     


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp 
            660                 665                 670         


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Ala Cys Leu Ala 
        675                 680                 685             


Leu Gly Gly Ala Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
    690                 695                 700                 


Gly Cys Ser Val Asp Phe 
705                 710 


<210>  702
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short

<400>  702

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  703
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short

<400>  703

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  704
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short

<400>  704

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly 
                325                 330                 335     


Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser 
            340                 345                 350         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        355                 360                 365             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    370                 375                 380                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
385                 390                 395                 400 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                405                 410                 415     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            420                 425                 430         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        435                 440                 445             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe 
    450                 455                 460                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
465                 470                 475                 480 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val 
                485                 490                 495     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            500                 505                 510         


Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met 
        515                 520                 525             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    530                 535                 540                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
545                 550                 555                 560 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr 
                565                 570                 575     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            580                 585                 590         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        595                 600                 605             


Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile 
    610                 615                 620                 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
625                 630                 635                 640 


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu 
                645                 650                 655     


Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly 
            660                 665                 670         


Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680             


<210>  705
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short

<400>  705

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  706
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short

<400>  706

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly Tyr Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Lys Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  707
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short

<400>  707

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His Arg Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly Tyr Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Lys Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  708
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short

<400>  708

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Thr Val 
                325                 330                 335     


Asn Asp Ile Gly Tyr Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Lys Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  709
<211>  681
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short

<400>  709

Thr Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Thr Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg 
            340                 345                 350         


Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu 
        355                 360                 365             


Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn 
    370                 375                 380                 


Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu 
385                 390                 395                 400 


Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys 
                405                 410                 415     


Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val 
            420                 425                 430         


Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly 
        435                 440                 445             


Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly 
    450                 455                 460                 


His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val 
465                 470                 475                 480 


Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala 
                485                 490                 495     


Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr 
            500                 505                 510         


Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr 
        515                 520                 525             


Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu 
    530                 535                 540                 


Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly 
545                 550                 555                 560 


Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His 
                565                 570                 575     


Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val 
            580                 585                 590         


Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe 
        595                 600                 605             


Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln 
    610                 615                 620                 


Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe 
625                 630                 635                 640 


Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr 
                645                 650                 655     


Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met 
            660                 665                 670         


Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680     


<210>  710
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short

<400>  710

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly 
                325                 330                 335     


Tyr Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser 
            340                 345                 350         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        355                 360                 365             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    370                 375                 380                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
385                 390                 395                 400 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                405                 410                 415     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            420                 425                 430         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        435                 440                 445             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe 
    450                 455                 460                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
465                 470                 475                 480 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val 
                485                 490                 495     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            500                 505                 510         


Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met 
        515                 520                 525             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    530                 535                 540                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
545                 550                 555                 560 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr 
                565                 570                 575     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            580                 585                 590         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        595                 600                 605             


Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile 
    610                 615                 620                 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
625                 630                 635                 640 


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu 
                645                 650                 655     


Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly 
            660                 665                 670         


Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680             


<210>  711
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short

<400>  711

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Arg Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  712
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short

<400>  712

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Leu Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  713
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short

<400>  713

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp Tyr Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  714
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short

<400>  714

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Thr Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  715
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short

<400>  715

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Ile Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  716
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short

<400>  716

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Leu Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  717
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short

<400>  717

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Thr Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  718
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short

<400>  718

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Val Val Ser Ala 
        675                 680                 685         


<210>  719
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short

<400>  719

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Ala Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Pro Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  720
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short

<400>  720

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Lys Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  721
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short

<400>  721

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Arg Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  722
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short

<400>  722

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Thr Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  723
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short

<400>  723

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Leu Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  724
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short

<400>  724

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Ile Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  725
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short

<400>  725

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Leu Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Ile Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  726
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short

<400>  726

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Ser Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Thr Ile Ser Asp Ile Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Ala Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  727
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short

<400>  727

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Leu Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  728
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short

<400>  728

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Ile Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  729
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short

<400>  729

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Val Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Phe Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  730
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short

<400>  730

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Lys Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  731
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short

<400>  731

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Lys 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  732
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short

<400>  732

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Glu Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  733
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short

<400>  733

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Ala Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Thr Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  734
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short

<400>  734

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Ala Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  735
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short

<400>  735

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Leu Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  736
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short

<400>  736

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Val Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  737
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short

<400>  737

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Ile Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ile 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  738
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short

<400>  738

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Ile Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  739
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short

<400>  739

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Gln Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  740
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short

<400>  740

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Ala Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  741
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short

<400>  741

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Ser Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Glu Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  742
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short

<400>  742

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Thr Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Gly Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  743
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short

<400>  743

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  744
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short

<400>  744

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Asn Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Met Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  745
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short

<400>  745

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Val Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  746
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short

<400>  746

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Asn Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Val Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  747
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short

<400>  747

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Ala Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  748
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short

<400>  748

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Val Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  749
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short

<400>  749

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp Tyr Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  750
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short

<400>  750

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Val 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Pro Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  751
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short

<400>  751

Thr Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Thr Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  752
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short

<400>  752

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Asn Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Val His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  753
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short

<400>  753

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  754
<211>  681
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short

<400>  754

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Asn Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg 
            340                 345                 350         


Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu 
        355                 360                 365             


Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn 
    370                 375                 380                 


Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu 
385                 390                 395                 400 


Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys 
                405                 410                 415     


Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val 
            420                 425                 430         


Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly 
        435                 440                 445             


Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly 
    450                 455                 460                 


His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val 
465                 470                 475                 480 


Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala 
                485                 490                 495     


Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr 
            500                 505                 510         


Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr 
        515                 520                 525             


Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu 
    530                 535                 540                 


Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly 
545                 550                 555                 560 


Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His 
                565                 570                 575     


Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val 
            580                 585                 590         


Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe 
        595                 600                 605             


Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln 
    610                 615                 620                 


Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe 
625                 630                 635                 640 


Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr 
                645                 650                 655     


Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met 
            660                 665                 670         


Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680     


<210>  755
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short

<400>  755

Thr Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  756
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short

<400>  756

Thr Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


Gln His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Val Cys Thr 
                485                 490                 495     


Ala Ala Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  757
<211>  681
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short

<400>  757

Thr Ser Ile Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg 
            340                 345                 350         


Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu 
        355                 360                 365             


Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn 
    370                 375                 380                 


Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu 
385                 390                 395                 400 


Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys 
                405                 410                 415     


Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val 
            420                 425                 430         


Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly 
        435                 440                 445             


Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly 
    450                 455                 460                 


His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val 
465                 470                 475                 480 


Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala 
                485                 490                 495     


Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr 
            500                 505                 510         


Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr 
        515                 520                 525             


Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu 
    530                 535                 540                 


Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly 
545                 550                 555                 560 


Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His 
                565                 570                 575     


Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val 
            580                 585                 590         


Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe 
        595                 600                 605             


Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln 
    610                 615                 620                 


Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe 
625                 630                 635                 640 


Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr 
                645                 650                 655     


Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met 
            660                 665                 670         


Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680     


<210>  758
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short

<400>  758

Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Ser 
            20                  25                  30          


Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn Lys 
        35                  40                  45              


Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Thr Arg Arg Ser Arg Arg Ser Val Ser Leu 
            100                 105                 110         


Arg Tyr His Tyr Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Lys Lys His Leu Ile Met Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Ile Val Ser Val Ala Ile Thr Trp Leu 
145                 150                 155                 160 


Met Gly Ser Leu Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Val 
                165                 170                 175     


Leu Ile Val Pro Ala Tyr Ser Ile Ser Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Leu Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Glu Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Met Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Leu Ser Asp Met Ala Ser Ala Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Pro Ser Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Ser Val Cys Lys Arg Thr Leu Gly Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Ile Phe Gly Lys Gly Ser Leu Val Thr Cys Ser Lys Phe Thr Cys 
    290                 295                 300                 


Cys Lys Lys Met Pro Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Pro Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Ser Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  759
<211>  686
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short

<400>  759

Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu 
1               5                   10                  15      


Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp 
            20                  25                  30          


Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys 
        35                  40                  45              


Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser 
    50                  55                  60                  


Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp 
65                  70                  75                  80  


Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His 
                85                  90                  95      


His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro 
            100                 105                 110         


Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu 
        115                 120                 125             


Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe 
    130                 135                 140                 


Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu 
145                 150                 155                 160 


Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu 
                165                 170                 175     


Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp 
            180                 185                 190         


Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu 
        195                 200                 205             


His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp 
    210                 215                 220                 


Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser 
225                 230                 235                 240 


Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys 
                245                 250                 255     


Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr 
            260                 265                 270         


Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly 
        275                 280                 285             


Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser 
    290                 295                 300                 


Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg 
305                 310                 315                 320 


Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn 
                325                 330                 335     


Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr 
            340                 345                 350         


Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu 
        355                 360                 365             


Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr 
    370                 375                 380                 


Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe 
385                 390                 395                 400 


His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro 
                405                 410                 415     


His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala 
            420                 425                 430         


Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His 
        435                 440                 445             


Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly 
    450                 455                 460                 


Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu 
465                 470                 475                 480 


Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe 
                485                 490                 495     


Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val 
            500                 505                 510         


Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala 
        515                 520                 525             


Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn 
    530                 535                 540                 


Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu 
545                 550                 555                 560 


Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys 
                565                 570                 575     


Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala 
            580                 585                 590         


Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp 
        595                 600                 605             


Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly 
    610                 615                 620                 


Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly 
625                 630                 635                 640 


Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp 
                645                 650                 655     


Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala 
            660                 665                 670         


Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685     


<210>  760
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short

<400>  760

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Arg Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  761
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short

<400>  761

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Met 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  762
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short

<400>  762

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Met Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
            500                 505                 510         


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685         


<210>  763
<211>  687
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short

<400>  763

Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala 
1               5                   10                  15      


Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn 
            20                  25                  30          


Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys 
        35                  40                  45              


Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr Met 
    50                  55                  60                  


Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val 
65                  70                  75                  80  


Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys 
                85                  90                  95      


His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu 
            100                 105                 110         


Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu 
        115                 120                 125             


Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile 
    130                 135                 140                 


Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu 
145                 150                 155                 160 


Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu 
                165                 170                 175     


Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg 
            180                 185                 190         


Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu 
        195                 200                 205             


Glu His Gly Gly Cys Val Thr Ala Met Ala Gln Asp Lys Pro Thr Val 
    210                 215                 220                 


Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg 
225                 230                 235                 240 


Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg 
                245                 250                 255     


Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln 
            260                 265                 270         


Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys 
        275                 280                 285             


Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys 
    290                 295                 300                 


Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr 
305                 310                 315                 320 


Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Leu Val 
                325                 330                 335     


Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile 
            340                 345                 350         


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
        355                 360                 365             


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
    370                 375                 380                 


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Ala His Lys Glu Trp 
385                 390                 395                 400 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Ala Thr Gly Thr 
                405                 410                 415     


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
            420                 425                 430         


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
        435                 440                 445             


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
    450                 455                 460                 


Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
465                 470                 475                 480 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
                485                 490                 495     


Phe Thr Lys Ile Pro Ala Glu Thr Val Asp Gly Thr Val Thr Val Glu 
            500                 505                 510         


Gly Gln Tyr Gly Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met 
        515                 520                 525             


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
    530                 535                 540                 


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
545                 550                 555                 560 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu 
                565                 570                 575     


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
            580                 585                 590         


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
        595                 600                 605             


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu 
    610                 615                 620                 


Gly Lys Gly Ile His Gln Ile Ile Gly Ala Ala Phe Lys Ser Leu Phe 
625                 630                 635                 640 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met 
                645                 650                 655     


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu 
            660                 665                 670         


Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Gly 
        675                 680                 685         


<210>  764
<211>  684
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short

<400>  764

Gly Ala Asp Ala Ser Ile Gly Ile Val Ser Leu Leu Leu Thr Thr Val 
1               5                   10                  15      


Met Ala Ala Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Ser Asp Ala Gly Lys Ala Ile Ser Phe Val Thr Thr Leu Gly 
        35                  40                  45              


Val Asn Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu 
    130                 135                 140                 


Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn 
            340                 345                 350         


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
        355                 360                 365             


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
    370                 375                 380                 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
385                 390                 395                 400 


Ile Pro Leu Pro Trp His Ser Gly Ala Asp Thr Glu Thr Pro His Trp 
                405                 410                 415     


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
            420                 425                 430         


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
        435                 440                 445             


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
    450                 455                 460                 


Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
465                 470                 475                 480 


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
                485                 490                 495     


Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
            500                 505                 510         


Ala Gly Arg Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp 
        515                 520                 525             


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
    530                 535                 540                 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
545                 550                 555                 560 


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile 
                565                 570                 575     


Thr His His Trp His Arg Ser Gly Ser Ile Ile Gly Lys Ala Phe Glu 
            580                 585                 590         


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
        595                 600                 605             


Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly 
    610                 615                 620                 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
625                 630                 635                 640 


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly 
                645                 650                 655     


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly 
            660                 665                 670         


Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 


<210>  765
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short

<400>  765

Gly Ala Asp Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      


Met Ala Ala Glu Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          


Asp Arg Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Met Gly 
        35                  40                  45              


Val Asn Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  


Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  


Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      


Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Pro Ser Arg Arg Ala 
            100                 105                 110         


Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             


Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu 
    130                 135                 140                 


Asn Trp Ile Leu Arg Asn Pro Gly Phe Thr Val Ala Ala Leu Ala Met 
145                 150                 155                 160 


Ala Trp Leu Leu Gly Asn Ser Thr Ser Gln Arg Val Ile Tyr Leu Val 
                165                 170                 175     


Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val 
            180                 185                 190         


Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp 
        195                 200                 205             


Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys 
    210                 215                 220                 


Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala 
225                 230                 235                 240 


Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser 
                245                 250                 255     


Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser 
            260                 265                 270         


Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly 
        275                 280                 285             


Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys 
    290                 295                 300                 


Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn 
305                 310                 315                 320 


Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly 
                325                 330                 335     


Met Ile Val Asn Asp Ile Gly His Glu Thr Asp Glu Asn Arg Ala Lys 
            340                 345                 350         


Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Ile Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Ser Lys Val Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Arg Asp Gly Pro Cys Lys Val Pro 
        515                 520                 525             


Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Ile Ile Thr Glu Ser Thr Glu Asn Ser Arg Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Val His Gln Val Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Ala Cys Leu Ala Leu Gly Gly Ala Met Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala 
    690 


<210>  766
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE

<400>  766

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  767
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE

<400>  767

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  768
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE

<400>  768

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu 
                165                 170                 175     


Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala 
            180                 185                 190         


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
        195                 200                 205             


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
    210                 215                 220                 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
225                 230                 235                 240 


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
                245                 250                 255     


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
            260                 265                 270         


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
        275                 280                 285             


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys 
    290                 295                 300                 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
305                 310                 315                 320 


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu 
                325                 330                 335     


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
            340                 345                 350         


Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
        355                 360                 365             


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
    370                 375                 380                 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
385                 390                 395                 400 


Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg 
                405                 410                 415     


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
            420                 425                 430         


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
        435                 440                 445         


<210>  769
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE

<400>  769

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  770
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE

<400>  770

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly Tyr 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  771
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE

<400>  771

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Thr Val Asn Asp Ile Gly Tyr 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  772
<211>  445
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE

<400>  772

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Glu Asn Arg 
                165                 170                 175     


Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
            180                 185                 190         


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 
        195                 200                 205             


Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 
    210                 215                 220                 


Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 
225                 230                 235                 240 


Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu 
                245                 250                 255     


Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 
            260                 265                 270         


Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 
        275                 280                 285             


Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg 
    290                 295                 300                 


Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 
305                 310                 315                 320 


Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly 
                325                 330                 335     


Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 
            340                 345                 350         


Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 
        355                 360                 365             


Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 
    370                 375                 380                 


Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 
385                 390                 395                 400 


Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly 
                405                 410                 415     


Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 
            420                 425                 430         


Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
        435                 440                 445 


<210>  773
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE

<400>  773

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu 
                165                 170                 175     


Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala 
            180                 185                 190         


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
        195                 200                 205             


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
    210                 215                 220                 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
225                 230                 235                 240 


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
                245                 250                 255     


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
            260                 265                 270         


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
        275                 280                 285             


Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser Gly His Leu Lys 
    290                 295                 300                 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
305                 310                 315                 320 


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu 
                325                 330                 335     


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
            340                 345                 350         


Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
        355                 360                 365             


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
    370                 375                 380                 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
385                 390                 395                 400 


Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg 
                405                 410                 415     


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
            420                 425                 430         


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
        435                 440                 445         


<210>  774
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE

<400>  774

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Leu Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  775
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE

<400>  775

Ser Lys Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  776
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE

<400>  776

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp Tyr Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  777
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE

<400>  777

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  778
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE

<400>  778

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Thr Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  779
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE

<400>  779

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Ile Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  780
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE

<400>  780

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Arg Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  781
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE

<400>  781

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Val Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  782
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE

<400>  782

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Ile Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  783
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE

<400>  783

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Ile Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  784
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE

<400>  784

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Leu Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  785
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE

<400>  785

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Thr Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  786
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE

<400>  786

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Leu Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  787
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE

<400>  787

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Glu Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  788
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE

<400>  788

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Ala Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  789
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE

<400>  789

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Ser Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Thr Ile Ser Asp Ile Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Ala Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  790
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE

<400>  790

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Ser Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Glu Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  791
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE

<400>  791

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Leu Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  792
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE

<400>  792

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Ile Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ile Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  793
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE

<400>  793

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Val Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  794
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE

<400>  794

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Ala Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  795
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE

<400>  795

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Thr Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Gly Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  796
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE

<400>  796

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Ala Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Leu Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Ala His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Ala Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Val Asp Gly Thr Val Thr Val Glu Gly Gln Tyr Gly Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  797
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE

<400>  797

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Ala Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  798
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE

<400>  798

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Asn Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  799
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE

<400>  799

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Ala Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  800
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE

<400>  800

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  801
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE

<400>  801

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp Tyr Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  802
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE

<400>  802

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  803
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE

<400>  803

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  804
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE

<400>  804

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Val Cys Thr Ala Ala Lys Val Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  805
<211>  445
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE

<400>  805

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Glu Asn Arg 
                165                 170                 175     


Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
            180                 185                 190         


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 
        195                 200                 205             


Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 
    210                 215                 220                 


Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ser Gly 
225                 230                 235                 240 


Ala Asp Thr Glu Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu 
                245                 250                 255     


Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 
            260                 265                 270         


Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 
        275                 280                 285             


Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg 
    290                 295                 300                 


Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 
305                 310                 315                 320 


Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly 
                325                 330                 335     


Thr Val Thr Val Glu Val Gln Tyr Ala Gly Arg Asp Gly Pro Cys Lys 
            340                 345                 350         


Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 
        355                 360                 365             


Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 
    370                 375                 380                 


Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 
385                 390                 395                 400 


Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly 
                405                 410                 415     


Ser Ile Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 
            420                 425                 430         


Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
        435                 440                 445 


<210>  806
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE

<400>  806

Ser Gln Lys Val Ile Tyr Leu Val Met Ile Val Leu Ile Val Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Ser Cys Ile Gly Val Ser Asn Arg Asp Leu Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Glu Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Met Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Leu Ser Asp Met Ala Ser Ala Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Pro Ser Leu Asp Lys Gln Ser Asp Thr Gln Ser Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Gly Asp Arg Gly Trp Gly Asn Gly Cys Gly Ile Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ser Lys Phe Thr Cys Cys Lys Lys Met Pro 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Pro 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Ser Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  807
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE

<400>  807

Arg Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  808
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE

<400>  808

Ser Gln Arg Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             


Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr 
    130                 135                 140                 


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 


Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His 
                165                 170                 175     


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro 
            180                 185                 190         


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser 
    290                 295                 300                 


Gly His Leu Lys Cys Arg Ile Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Ser Lys Val Pro 
                325                 330                 335     


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         


Arg Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Ile Ile Thr 
    370                 375                 380                 


Glu Ser Thr Glu Asn Ser Arg Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                405                 410                 415     


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
            420                 425                 430         


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
        435                 440                 445             


Phe Gly Ser 
    450     


<210>  809
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS wild type

<400>  809
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  810
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS wild type

<400>  810
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacgag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca cgugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacu guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaaucuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacgc aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  811
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS wild type

<400>  811
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa     1080

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaaguuua     1140

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug     1200

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauuuaag     1320

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac     1380

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu     1440

ggccauuuga aaugccgccu aaaaauggac aagcucagau ugaagggcgu gucauauucc     1500

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1560

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1620

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1680

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1740

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1800

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1860

acagccuggg acuucggauc agucgggggu guguucaacu cacuggguaa gggcauucac     1920

cagauuuuug gagcagccuu caaaucacug uuuggaggaa uguccugguu cucacagauc     1980

cucauaggca cgcugcuagu gugguuaggu uugaacacaa agaauggauc uaucucccuc     2040

acaugcuugg cccugggggg agugaugauc uuccucucca cggcuguuuc ugcugacgug     2100

gggugcucag uggacuucuc aaaa                                            2124


<210>  812
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) CDS wild type

<400>  812
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guccacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  813
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS wild type

<400>  813
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  814
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) CDS wild type

<400>  814
acgagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacacgugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucacuguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg ggggguuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu ggcugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa aucuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacgcaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  815
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) CDS wild type

<400>  815
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag ucgacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaacgg uuguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugauuggaua ugaaacugac     1020

gaagauagag cgaaagucga gguuacgccu aauucaccaa gagcggaagc aaccuuggga     1080

ggcuuuggaa guuuaggacu ugacugugaa ccaaggacag gccuugacuu uucagaucug     1140

uauuaccuga ccaugaacaa uaagcauugg uuggugcaca aagagugguu ucaugacauc     1200

ccauugccuu ggcaugcugg ggcagacacc ggaacuccac acuggaacaa caaagaggca     1260

uugguagaau uuaaggaugc ccacgccaag aggcaaaccg ucgucguucu ggggagccag     1320

gaaggagccg uucacacggc ucucgcugga gcucuagagg cugagaugga uggugcaaag     1380

ggaaggcugu ucucuggcca uuugaaaugc cgccuaaaaa uggacaagcu cagauugaag     1440

ggcgugucau auuccuugug cacugcggca uucacauuca ccaagguccc agcugaaaca     1500

cugcauggaa cagucacagu ggaggugcag uaugcaggga cagauggacc cugcaagauc     1560

ccaguccaga uggcggugga caugcagacc cugaccccag uuggaaggcu gauaaccgcc     1620

aaccccguga uuacugaaag cacugagaac ucaaagauga uguuggagcu ugacccacca     1680

uuuggggauu cuuacauugu cauaggaguu ggggacaaga aaaucaccca ccacuggcau     1740

aggaguggua gcaccaucgg aaaggcauuu gaggccacug ugagaggcgc caagagaaug     1800

gcaguccugg gggauacagc cugggacuuc ggaucagucg gggguguguu caacucacug     1860

gguaagggca uucaccagau uuuuggagca gccuucaaau cacuguuugg aggaaugucc     1920

ugguucucac agauccucau aggcacgcug cuaguguggu uagguuugaa cacaaagaau     1980

ggaucuaucu cccucacaug cuuggcccug gggggaguga ugaucuuccu cuccacggcu     2040

guuucugcu                                                             2049


<210>  816
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) CDS wild type

<400>  816
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguuggucca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  817
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS wild type

<400>  817
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  818
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS wild type

<400>  818
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacu guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  819
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS wild type

<400>  819
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      180

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      360

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      420

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca      480

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa      540

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaaguuua      600

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug      660

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau      720

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauuuaag      780

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac      840

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu      900

ggccauuuga aaugccgccu aaaaauggac aagcucagau ugaagggcgu gucauauucc      960

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1020

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1080

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1140

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1200

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1260

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1320

acagccuggg acuucggauc a                                               1341


<210>  820
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) CDS wild type

<400>  820
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guccacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  821
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) CDS wild 
       type

<400>  821
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uccggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  822
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) CDS wild type

<400>  822
acgagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacacgugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucacuguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uccggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg ggggguuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu ggcugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa aucuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacgcaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  823
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) CDS wild type

<400>  823
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag ucgacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaacgg uuguggacuu uccggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugauuggaua ugaaacugac     1020

gaagauagag cgaaagucga gguuacgccu aauucaccaa gagcggaagc aaccuuggga     1080

ggcuuuggaa guuuaggacu ugacugugaa ccaaggacag gccuugacuu uucagaucug     1140

uauuaccuga ccaugaacaa uaagcauugg uuggugcaca aagagugguu ucaugacauc     1200

ccauugccuu ggcaugcugg ggcagacacc ggaacuccac acuggaacaa caaagaggca     1260

uugguagaau uuaaggaugc ccacgccaag aggcaaaccg ucgucguucu ggggagccag     1320

gaaggagccg uucacacggc ucucgcugga gcucuagagg cugagaugga uggugcaaag     1380

ggaaggcugu ucucuggcca uuugaaaugc cgccuaaaaa uggacaagcu cagauugaag     1440

ggcgugucau auuccuugug cacugcggca uucacauuca ccaagguccc agcugaaaca     1500

cugcauggaa cagucacagu ggaggugcag uaugcaggga cagauggacc cugcaagauc     1560

ccaguccaga uggcggugga caugcagacc cugaccccag uuggaaggcu gauaaccgcc     1620

aaccccguga uuacugaaag cacugagaac ucaaagauga uguuggagcu ugacccacca     1680

uuuggggauu cuuacauugu cauaggaguu ggggacaaga aaaucaccca ccacuggcau     1740

aggaguggua gcaccaucgg aaaggcauuu gaggccacug ugagaggcgc caagagaaug     1800

gcaguccugg gggauacagc cugggacuuc ggaucagucg gggguguguu caacucacug     1860

gguaagggca uucaccagau uuuuggagca gccuucaaau cacuguuugg aggaaugucc     1920

ugguucucac agauccucau aggcacgcug cuaguguggu uagguuugaa cacaaagaau     1980

ggaucuaucu cccucacaug cuuggcccug gggggaguga ugaucuuccu cuccacggcu     2040

guuucugcu                                                             2049


<210>  824
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) CDS wild type

<400>  824
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uccggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguuggucca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  825
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) CDS wild 
       type

<400>  825
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuca ggacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  826
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) CDS wild type

<400>  826
acgagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacacgugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucacuguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuca ggacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg ggggguuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu ggcugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa aucuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacgcaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  827
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) CDS wild type

<400>  827
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuca ggacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguuggucca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  828
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS wild type

<400>  828
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcacgcug ggcaaggccu uuucaacaac uuugaaggga     1800

gcucaaagac uggcagcguu gggcgacaca gccugggacu uuggcucuau uggagggguc     1860

uucaacucca uaggaagagc cguucaccaa guguuuggug gugccuucag aacacucuuu     1920

gggggaaugu cuuggaucac acaagggcua augggugccc uacugcucug gaugggcguc     1980

aacgcacgag accgaucaau ugcuuuggcc uucuuagcca caggaggugu gcucguguuc     2040

uuagcgacca augugcacgc c                                               2061


<210>  829
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS wild type

<400>  829
acgagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacacgugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucacuguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg ggggguuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu ggcugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcacgcug ggcaaggccu uuucaacaac uuugaaggga     1800

gcucaaagac uggcagcguu gggcgacaca gccugggacu uuggcucuau uggagggguc     1860

uucaacucca uaggaagagc cguucaccaa guguuuggug gugccuucag aacacucuuu     1920

gggggaaugu cuuggaucac acaagggcua augggugccc uacugcucug gaugggcguc     1980

aacgcacgag accgaucaau ugcuuuggcc uucuuagcca caggaggugu gcucguguuc     2040

uuagcgacca augugcacgc c                                               2061


<210>  830
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       CDS wild type

<400>  830
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag ucgacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaacgg uuguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugauuggaua ugaaacugac     1020

gaagauagag cgaaagucga gguuacgccu aauucaccaa gagcggaagc aaccuuggga     1080

ggcuuuggaa guuuaggacu ugacugugaa ccaaggacag gccuugacuu uucagaucug     1140

uauuaccuga ccaugaacaa uaagcauugg uuggugcaca aagagugguu ucaugacauc     1200

ccauugccuu ggcaugcugg ggcagacacc ggaacuccac acuggaacaa caaagaggca     1260

uugguagaau uuaaggaugc ccacgccaag aggcaaaccg ucgucguucu ggggagccag     1320

gaaggagccg uucacacggc ucucgcugga gcucuagagg cugagaugga uggugcaaag     1380

ggaaggcugu ucucuggcca uuugaaaugc cgccuaaaaa uggacaagcu cagauugaag     1440

ggcgugucau auuccuugug cacugcggca uucacauuca ccaagguccc agcugaaaca     1500

cugcauggaa cagucacagu ggaggugcag uaugcaggga cagauggacc cugcaagauc     1560

ccaguccaga uggcggugga caugcagacc cugaccccag uuggaaggcu gauaaccgcc     1620

aaccccguga uuacugaaag cacugagaac ucaaagauga uguuggagcu ugacccacca     1680

uuuggggauu cuuacauugu cauaggaguu ggggacaaga aaaucaccca ccacuggcau     1740

aggaguggca gcacgcuggg caaggccuuu ucaacaacuu ugaagggagc ucaaagacug     1800

gcagcguugg gcgacacagc cugggacuuu ggcucuauug gaggggucuu caacuccaua     1860

ggaagagccg uucaccaagu guuugguggu gccuucagaa cacucuuugg gggaaugucu     1920

uggaucacac aagggcuaau gggugcccua cugcucugga ugggcgucaa cgcacgagac     1980

cgaucaauug cuuuggccuu cuuagccaca ggaggugugc ucguguucuu agcgaccaau     2040

gugcacgcc                                                             2049


<210>  831
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS wild type

<400>  831
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguuggucca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcacgcug ggcaaggccu uuucaacaac uuugaaggga     1800

gcucaaagac uggcagcguu gggcgacaca gccugggacu uuggcucuau uggagggguc     1860

uucaacucca uaggaagagc cguucaccaa guguuuggug gugccuucag aacacucuuu     1920

gggggaaugu cuuggaucac acaagggcua augggugccc uacugcucug gaugggcguc     1980

aacgcacgag accgaucaau ugcuuuggcc uucuuagcca caggaggugu gcucguguuc     2040

uuagcgacca augugcacgc c                                               2061


<210>  832
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS wild type

<400>  832
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  833
<211>  2133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  833
auaaucaaug cuaggaagga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugaugugg ggugcucggu ggacuucuca aag                                  2133


<210>  834
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  834
aucaaugcua ggaaggagaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gauguggggu gcucggugga cuucucaaag                                      2130


<210>  835
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  835
aaugcuagga aggagaagaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100

guggggugcu cgguggacuu cucaaag                                         2127


<210>  836
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  836
gcuaggaagg agaagaagag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugaugug     2100

gggugcucgg uggacuucuc aaag                                            2124


<210>  837
<211>  2121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  837
aggaaggaga agaagagacg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcucggugg acuucucaaa g                                               2121


<210>  838
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  838
aaggagaaga agagacgggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga uguggggugc     2100

ucgguggacu ucucaaag                                                   2118


<210>  839
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  839
gagaagaaga gacggggcgc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcucg     2100

guggacuucu caaag                                                      2115


<210>  840
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  840
aagaagagac ggggcgcaga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     2100

gacuucucaa ag                                                         2112


<210>  841
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  841
aagagacggg gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cucgguggac     2100

uucucaaag                                                             2109


<210>  842
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  842
agacggggcg cagauaccag ugucggaauu guuggccucc ugcugaccac agcuauggca       60

gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg      120

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      180

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      240

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      300

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      360

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      420

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      480

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      540

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      600

gaagguaugu caggugggac uuggguugau auugucuugg aacauggagg uugugucacc      660

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      720

auggcggagg uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc      780

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      840

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu      900

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      960

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc     1020

guuaaugaca caggacauga aacugaugag aauagagcga agguugagau aacgcccaau     1080

ucaccaagag ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg     1140

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug     1200

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1260

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1320

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1380

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1440

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc     1500

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1560

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1620

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1680

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1740

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1800

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1860

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu     1920

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1980

augugguugg gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     2040

ggaguguuga ucuucuuauc cacagccguc ucugcugaug uggggugcuc gguggacuuc     2100

ucaaag                                                                2106


<210>  843
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  843
cggggcgcag auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg       60

gaggucacua gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag      120

gccauaucuu uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu      180

ggacacaugu gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa      240

ccagaugacg ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc      300

caucacaaaa aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc      360

acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac      420

uugauuagag ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc      480

aucgcuuggc uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug      540

cugauugccc cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa      600

gguaugucag gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua      660

auggcacagg acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug      720

gcggagguaa gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc      780

ugcccaacac aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa      840

agaacguuag uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug      900

gugacaugcg cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag      960

aaucuggagu accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu     1020

aaugacacag gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca     1080

ccaagagccg aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg     1140

acaggccuug acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu     1200

cacaaggagu gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa     1320

acugucgugg uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug     1380

gaggcugaga uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug     1440

aaaauggaua aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca     1500

uucaccaaga ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca     1560

gggacagaug gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc     1620

ccaguuggga gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag     1680

augaugcugg aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag     1740

aagaagauca cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc     1800

acugugagag gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca     1860

guuggaggcg cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc     1920

aaaucauugu uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug     1980

ugguuggguc ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga     2040

guguugaucu ucuuauccac agccgucucu gcugaugugg ggugcucggu ggacuucuca     2100

aag                                                                   2103


<210>  844
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  844
ggcgcagaua ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag       60

gucacuagac gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc      120

auaucuuuuc caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga      180

cacaugugug augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca      240

gaugacgucg auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau      300

cacaaaaaag gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu      360

aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug      420

auuagagucg aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc      480

gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu      600

augucaggug ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug      660

gcacaggaca aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg      720

gagguaagau ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acguuagugg acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug      900

acaugcgcua aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau      960

cuggaguacc ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau     1020

gacacaggac augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca     1080

agagccgaag ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca     1140

ggccuugacu uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac     1200

aaggaguggu uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu     1320

gucgugguuc uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag     1380

gcugagaugg auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa     1440

auggauaaac uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc     1500

accaagaucc cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg     1560

acagauggac cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca     1620

guugggaggu ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug     1680

augcuggaac uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag     1740

aagaucaccc accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu     1800

gugagaggug ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa     1920

ucauuguuug gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg     1980

uugggucuga acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug     2040

uugaucuucu uauccacagc cgucucugcu gauguggggu gcucggugga cuucucaaag     2100


<210>  845
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  845
gcagauacca gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc       60

acuagacgug ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua      120

ucuuuuccaa ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac      180

augugugaug ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau      240

gacgucgauu guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac      300

aaaaaaggug aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg      360

aagcugcaaa cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu      420

agagucgaaa auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu      480

uggcuuuugg gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu      540

gccccggcau acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug      600

ucagguggga cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca      660

caggacaaac cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag      720

guaagauccu acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca      780

acacaaggug aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg      840

uuaguggaca gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca      900

ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug      960

gaguaccgga uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac     1020

acaggacaug aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga     1080

gccgaagcca cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc     1140

cuugacuuuu cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag     1200

gagugguucc acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc     1320

gugguucuag ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu     1380

gagauggaug gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug     1440

gauaaacuua gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc     1500

aagaucccgg cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca     1560

gauggaccuu gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu     1620

gggagguuga uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug     1680

cuggaacuug auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag     1740

aucacccacc acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug     1800

agaggugcca agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga     1860

ggcgcucuca acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca     1920

uuguuuggag gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug     1980

ggucugaaca caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug     2040

aucuucuuau ccacagccgu cucugcugau guggggugcu cgguggacuu cucaaag        2097


<210>  846
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  846
gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1800

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1860

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1920

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     1980

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2040

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaag           2094


<210>  847
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  847
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc ugaugugggg ugcucggugg acuucucaaa g              2091


<210>  848
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  848
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcggc cgucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc ugaugugggg ugcucggugg acuucucaaa g              2091


<210>  849
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  849
cggggcgcag auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg       60

gccgucacua gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag      120

gccauaucuu uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu      180

ggacacaugu gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa      240

ccagaugacg ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc      300

caucacaaaa aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc      360

acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac      420

uugauuagag ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc      480

aucgcuuggc uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug      540

cugauugccc cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa      600

gguaugucag gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua      660

auggcacagg acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug      720

gcggagguaa gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc      780

ugcccaacac aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa      840

agaacguuag uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug      900

gugacaugcg cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag      960

aaucuggagu accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu     1020

aaugacacag gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca     1080

ccaagagccg aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg     1140

acaggccuug acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu     1200

cacaaggagu gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa     1320

acugucgugg uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug     1380

gaggcugaga uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug     1440

aaaauggaua aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca     1500

uucaccaaga ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca     1560

gggacagaug gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc     1620

ccaguuggga gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag     1680

augaugcugg aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag     1740

aagaagauca cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc     1800

acugugagag gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca     1860

guuggaggcg cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc     1920

aaaucauugu uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug     1980

ugguuggguc ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga     2040

guguugaucu ucuuauccac agccgucucu gcugaugugg ggugcucggu ggacuucuca     2100

aag                                                                   2103


<210>  850
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  850
agaggauugu ccucaaggaa acgccguucc caugauguuc ugacugugca auuccuaauu       60

uugggaaugc uguugaugac ggguggagug acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu ccgucgacuu cagcaag                                         2127


<210>  851
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  851
agaggauugu ccucaaggaa acgccguucc caugauguuc ugacugugca auuccuaauu       60

uugggaaugc uguugaugac gggugccgug acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu ccgucgacuu cagcaag                                         2127


<210>  852
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  852
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugc                                                   2118


<210>  853
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  853
auaaucaaug cuaggaagga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugaugugg ggugc                                                      2115


<210>  854
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  854
aucaaugcua ggaaggagaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gauguggggu gc                                                         2112


<210>  855
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  855
aaugcuagga aggagaagaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100

guggggugc                                                             2109


<210>  856
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  856
gcuaggaagg agaagaagag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugaugug     2100

gggugc                                                                2106


<210>  857
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  857
aggaaggaga agaagagacg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugc                                                                   2103


<210>  858
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  858
aaggagaaga agagacgggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga uguggggugc     2100


<210>  859
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  859
gagaagaaga gacggggcgc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugc        2097


<210>  860
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  860
aagaagagac ggggcgcaga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugc           2094


<210>  861
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  861
aagagacggg gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug c              2091


<210>  862
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  862
agacggggcg cagauaccag ugucggaauu guuggccucc ugcugaccac agcuauggca       60

gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg      120

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      180

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      240

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      300

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      360

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      420

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      480

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      540

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      600

gaagguaugu caggugggac uuggguugau auugucuugg aacauggagg uugugucacc      660

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      720

auggcggagg uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc      780

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      840

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu      900

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      960

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc     1020

guuaaugaca caggacauga aacugaugag aauagagcga agguugagau aacgcccaau     1080

ucaccaagag ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg     1140

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug     1200

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1260

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1320

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1380

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1440

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc     1500

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1560

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1620

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1680

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1740

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1800

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1860

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu     1920

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1980

augugguugg gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     2040

ggaguguuga ucuucuuauc cacagccguc ucugcugaug uggggugc                  2088


<210>  863
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  863
cggggcgcag auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg       60

gaggucacua gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag      120

gccauaucuu uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu      180

ggacacaugu gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa      240

ccagaugacg ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc      300

caucacaaaa aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc      360

acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac      420

uugauuagag ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc      480

aucgcuuggc uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug      540

cugauugccc cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa      600

gguaugucag gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua      660

auggcacagg acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug      720

gcggagguaa gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc      780

ugcccaacac aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa      840

agaacguuag uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug      900

gugacaugcg cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag      960

aaucuggagu accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu     1020

aaugacacag gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca     1080

ccaagagccg aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg     1140

acaggccuug acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu     1200

cacaaggagu gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa     1320

acugucgugg uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug     1380

gaggcugaga uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug     1440

aaaauggaua aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca     1500

uucaccaaga ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca     1560

gggacagaug gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc     1620

ccaguuggga gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag     1680

augaugcugg aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag     1740

aagaagauca cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc     1800

acugugagag gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca     1860

guuggaggcg cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc     1920

aaaucauugu uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug     1980

ugguuggguc ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga     2040

guguugaucu ucuuauccac agccgucucu gcugaugugg ggugc                     2085


<210>  864
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  864
ggcgcagaua ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag       60

gucacuagac gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc      120

auaucuuuuc caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga      180

cacaugugug augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca      240

gaugacgucg auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau      300

cacaaaaaag gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu      360

aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug      420

auuagagucg aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc      480

gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu      600

augucaggug ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug      660

gcacaggaca aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg      720

gagguaagau ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acguuagugg acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug      900

acaugcgcua aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau      960

cuggaguacc ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau     1020

gacacaggac augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca     1080

agagccgaag ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca     1140

ggccuugacu uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac     1200

aaggaguggu uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu     1320

gucgugguuc uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag     1380

gcugagaugg auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa     1440

auggauaaac uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc     1500

accaagaucc cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg     1560

acagauggac cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca     1620

guugggaggu ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug     1680

augcuggaac uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag     1740

aagaucaccc accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu     1800

gugagaggug ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa     1920

ucauuguuug gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg     1980

uugggucuga acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug     2040

uugaucuucu uauccacagc cgucucugcu gauguggggu gc                        2082


<210>  865
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  865
gcagauacca gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc       60

acuagacgug ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua      120

ucuuuuccaa ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac      180

augugugaug ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau      240

gacgucgauu guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac      300

aaaaaaggug aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg      360

aagcugcaaa cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu      420

agagucgaaa auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu      480

uggcuuuugg gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu      540

gccccggcau acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug      600

ucagguggga cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca      660

caggacaaac cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag      720

guaagauccu acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca      780

acacaaggug aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg      840

uuaguggaca gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca      900

ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug      960

gaguaccgga uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac     1020

acaggacaug aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga     1080

gccgaagcca cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc     1140

cuugacuuuu cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag     1200

gagugguucc acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc     1320

gugguucuag ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu     1380

gagauggaug gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug     1440

gauaaacuua gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc     1500

aagaucccgg cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca     1560

gauggaccuu gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu     1620

gggagguuga uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug     1680

cuggaacuug auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag     1740

aucacccacc acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug     1800

agaggugcca agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga     1860

ggcgcucuca acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca     1920

uuguuuggag gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug     1980

ggucugaaca caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug     2040

aucuucuuau ccacagccgu cucugcugau guggggugc                            2079


<210>  866
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  866
gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1800

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1860

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1920

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     1980

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2040

uucuuaucca cagccgucuc ugcugaugug gggugc                               2076


<210>  867
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  867
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc ugaugugggg ugc                                  2073


<210>  868
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  868
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcggc cgucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc ugaugugggg ugc                                  2073


<210>  869
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  869
cggggcgcag auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg       60

gccgucacua gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag      120

gccauaucuu uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu      180

ggacacaugu gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa      240

ccagaugacg ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc      300

caucacaaaa aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc      360

acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac      420

uugauuagag ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc      480

aucgcuuggc uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug      540

cugauugccc cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa      600

gguaugucag gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua      660

auggcacagg acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug      720

gcggagguaa gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc      780

ugcccaacac aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa      840

agaacguuag uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug      900

gugacaugcg cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag      960

aaucuggagu accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu     1020

aaugacacag gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca     1080

ccaagagccg aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg     1140

acaggccuug acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu     1200

cacaaggagu gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa     1320

acugucgugg uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug     1380

gaggcugaga uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug     1440

aaaauggaua aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca     1500

uucaccaaga ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca     1560

gggacagaug gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc     1620

ccaguuggga gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag     1680

augaugcugg aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag     1740

aagaagauca cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc     1800

acugugagag gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca     1860

guuggaggcg cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc     1920

aaaucauugu uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug     1980

ugguuggguc ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga     2040

guguugaucu ucuuauccac agccgucucu gcugaugugg ggugc                     2085


<210>  870
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  870
agaggauugu ccucaaggaa acgccguucc caugauguuc ugacugugca auuccuaauu       60

uugggaaugc uguugaugac ggguggagug acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugc                                                             2109


<210>  871
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  871
agaggauugu ccucaaggaa acgccguucc caugauguuc ugacugugca auuccuaauu       60

uugggaaugc uguugaugac gggugccgug acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugc                                                             2109


<210>  872
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  872
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugau                                                             2109


<210>  873
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  873
auaaucaaug cuaggaagga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugau                                                                2106


<210>  874
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  874
aucaaugcua ggaaggagaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gau                                                                   2103


<210>  875
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  875
aaugcuagga aggagaagaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100


<210>  876
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  876
gcuaggaagg agaagaagag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugau        2097


<210>  877
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  877
aggaaggaga agaagagacg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugau           2094


<210>  878
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  878
aaggagaaga agagacgggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga u              2091


<210>  879
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  879
gagaagaaga gacggggcgc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugau                  2088


<210>  880
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  880
aagaagagac ggggcgcaga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugau                     2085


<210>  881
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  881
aagagacggg gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug au                        2082


<210>  882
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  882
agacggggcg cagauaccag ugucggaauu guuggccucc ugcugaccac agcuauggca       60

gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg      120

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      180

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      240

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      300

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      360

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      420

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      480

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      540

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      600

gaagguaugu caggugggac uuggguugau auugucuugg aacauggagg uugugucacc      660

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      720

auggcggagg uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc      780

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      840

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu      900

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      960

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc     1020

guuaaugaca caggacauga aacugaugag aauagagcga agguugagau aacgcccaau     1080

ucaccaagag ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg     1140

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug     1200

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1260

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1320

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1380

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1440

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc     1500

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1560

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1620

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1680

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1740

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1800

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1860

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu     1920

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1980

augugguugg gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     2040

ggaguguuga ucuucuuauc cacagccguc ucugcugau                            2079


<210>  883
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  883
cggggcgcag auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg       60

gaggucacua gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag      120

gccauaucuu uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu      180

ggacacaugu gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa      240

ccagaugacg ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc      300

caucacaaaa aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc      360

acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac      420

uugauuagag ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc      480

aucgcuuggc uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug      540

cugauugccc cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa      600

gguaugucag gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua      660

auggcacagg acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug      720

gcggagguaa gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc      780

ugcccaacac aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa      840

agaacguuag uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug      900

gugacaugcg cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag      960

aaucuggagu accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu     1020

aaugacacag gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca     1080

ccaagagccg aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg     1140

acaggccuug acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu     1200

cacaaggagu gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa     1320

acugucgugg uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug     1380

gaggcugaga uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug     1440

aaaauggaua aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca     1500

uucaccaaga ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca     1560

gggacagaug gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc     1620

ccaguuggga gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag     1680

augaugcugg aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag     1740

aagaagauca cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc     1800

acugugagag gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca     1860

guuggaggcg cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc     1920

aaaucauugu uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug     1980

ugguuggguc ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga     2040

guguugaucu ucuuauccac agccgucucu gcugau                               2076


<210>  884
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  884
ggcgcagaua ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag       60

gucacuagac gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc      120

auaucuuuuc caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga      180

cacaugugug augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca      240

gaugacgucg auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau      300

cacaaaaaag gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu      360

aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug      420

auuagagucg aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc      480

gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu      600

augucaggug ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug      660

gcacaggaca aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg      720

gagguaagau ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acguuagugg acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug      900

acaugcgcua aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau      960

cuggaguacc ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau     1020

gacacaggac augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca     1080

agagccgaag ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca     1140

ggccuugacu uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac     1200

aaggaguggu uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu     1320

gucgugguuc uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag     1380

gcugagaugg auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa     1440

auggauaaac uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc     1500

accaagaucc cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg     1560

acagauggac cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca     1620

guugggaggu ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug     1680

augcuggaac uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag     1740

aagaucaccc accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu     1800

gugagaggug ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa     1920

ucauuguuug gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg     1980

uugggucuga acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug     2040

uugaucuucu uauccacagc cgucucugcu gau                                  2073


<210>  885
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  885
gcagauacca gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc       60

acuagacgug ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua      120

ucuuuuccaa ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac      180

augugugaug ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau      240

gacgucgauu guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac      300

aaaaaaggug aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg      360

aagcugcaaa cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu      420

agagucgaaa auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu      480

uggcuuuugg gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu      540

gccccggcau acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug      600

ucagguggga cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca      660

caggacaaac cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag      720

guaagauccu acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca      780

acacaaggug aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg      840

uuaguggaca gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca      900

ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug      960

gaguaccgga uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac     1020

acaggacaug aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga     1080

gccgaagcca cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc     1140

cuugacuuuu cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag     1200

gagugguucc acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc     1320

gugguucuag ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu     1380

gagauggaug gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug     1440

gauaaacuua gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc     1500

aagaucccgg cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca     1560

gauggaccuu gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu     1620

gggagguuga uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug     1680

cuggaacuug auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag     1740

aucacccacc acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug     1800

agaggugcca agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga     1860

ggcgcucuca acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca     1920

uuguuuggag gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug     1980

ggucugaaca caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug     2040

aucuucuuau ccacagccgu cucugcugau                                      2070


<210>  886
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  886
gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1800

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1860

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1920

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     1980

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2040

uucuuaucca cagccgucuc ugcugau                                         2067


<210>  887
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  887
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc ugau                                            2064


<210>  888
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  888
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcggc cgucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc ugau                                            2064


<210>  889
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  889
cggggcgcag auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg       60

gccgucacua gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag      120

gccauaucuu uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu      180

ggacacaugu gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa      240

ccagaugacg ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc      300

caucacaaaa aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc      360

acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac      420

uugauuagag ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc      480

aucgcuuggc uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug      540

cugauugccc cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa      600

gguaugucag gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua      660

auggcacagg acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug      720

gcggagguaa gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc      780

ugcccaacac aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa      840

agaacguuag uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug      900

gugacaugcg cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag      960

aaucuggagu accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu     1020

aaugacacag gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca     1080

ccaagagccg aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg     1140

acaggccuug acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu     1200

cacaaggagu gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa     1320

acugucgugg uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug     1380

gaggcugaga uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug     1440

aaaauggaua aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca     1500

uucaccaaga ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca     1560

gggacagaug gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc     1620

ccaguuggga gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag     1680

augaugcugg aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag     1740

aagaagauca cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc     1800

acugugagag gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca     1860

guuggaggcg cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc     1920

aaaucauugu uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug     1980

ugguuggguc ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga     2040

guguugaucu ucuuauccac agccgucucu gcugau                               2076


<210>  890
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  890
agaggauugu ccucaaggaa acgccguucc caugauguuc ugacugugca auuccuaauu       60

uugggaaugc uguugaugac ggguggagug acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100


<210>  891
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  891
agaggauugu ccucaaggaa acgccguucc caugauguuc ugacugugca auuccuaauu       60

uugggaaugc uguugaugac gggugccgug acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100


<210>  892
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  892
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcu                                                                2106


<210>  893
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  893
aucaaugcua ggaaggagaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100


<210>  894
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  894
auaaucaaug cuaggaagga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcu                                                                   2103


<210>  895
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  895
aaugcuagga aggagaagaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcu        2097


<210>  896
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  896
gcuaggaagg agaagaagag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcu           2094


<210>  897
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  897
aggaaggaga agaagagacg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc u              2091


<210>  898
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  898
aaggagaaga agagacgggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcu                  2088


<210>  899
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  899
gagaagaaga gacggggcgc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcu                     2085


<210>  900
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  900
aagaagagac ggggcgcaga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cu                        2082


<210>  901
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  901
aagagacggg gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcu                            2079


<210>  902
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  902
agacggggcg cagauaccag ugucggaauu guuggccucc ugcugaccac agcuauggca       60

gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg      120

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      180

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      240

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      300

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      360

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      420

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      480

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      540

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      600

gaagguaugu caggugggac uuggguugau auugucuugg aacauggagg uugugucacc      660

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      720

auggcggagg uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc      780

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      840

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu      900

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      960

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc     1020

guuaaugaca caggacauga aacugaugag aauagagcga agguugagau aacgcccaau     1080

ucaccaagag ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg     1140

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug     1200

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1260

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1320

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1380

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1440

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc     1500

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1560

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1620

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1680

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1740

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1800

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1860

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu     1920

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1980

augugguugg gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     2040

ggaguguuga ucuucuuauc cacagccguc ucugcu                               2076


<210>  903
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  903
cggggcgcag auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg       60

gaggucacua gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag      120

gccauaucuu uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu      180

ggacacaugu gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa      240

ccagaugacg ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc      300

caucacaaaa aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc      360

acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac      420

uugauuagag ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc      480

aucgcuuggc uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug      540

cugauugccc cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa      600

gguaugucag gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua      660

auggcacagg acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug      720

gcggagguaa gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc      780

ugcccaacac aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa      840

agaacguuag uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug      900

gugacaugcg cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag      960

aaucuggagu accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu     1020

aaugacacag gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca     1080

ccaagagccg aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg     1140

acaggccuug acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu     1200

cacaaggagu gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa     1320

acugucgugg uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug     1380

gaggcugaga uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug     1440

aaaauggaua aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca     1500

uucaccaaga ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca     1560

gggacagaug gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc     1620

ccaguuggga gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag     1680

augaugcugg aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag     1740

aagaagauca cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc     1800

acugugagag gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca     1860

guuggaggcg cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc     1920

aaaucauugu uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug     1980

ugguuggguc ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga     2040

guguugaucu ucuuauccac agccgucucu gcu                                  2073


<210>  904
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  904
ggcgcagaua ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag       60

gucacuagac gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc      120

auaucuuuuc caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga      180

cacaugugug augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca      240

gaugacgucg auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau      300

cacaaaaaag gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu      360

aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug      420

auuagagucg aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc      480

gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug      540

auugccccgg cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu      600

augucaggug ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug      660

gcacaggaca aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg      720

gagguaagau ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acguuagugg acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug      900

acaugcgcua aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau      960

cuggaguacc ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau     1020

gacacaggac augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca     1080

agagccgaag ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca     1140

ggccuugacu uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac     1200

aaggaguggu uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu     1320

gucgugguuc uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag     1380

gcugagaugg auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa     1440

auggauaaac uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc     1500

accaagaucc cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg     1560

acagauggac cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca     1620

guugggaggu ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug     1680

augcuggaac uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag     1740

aagaucaccc accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu     1800

gugagaggug ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa     1920

ucauuguuug gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg     1980

uugggucuga acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug     2040

uugaucuucu uauccacagc cgucucugcu                                      2070


<210>  905
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  905
gcagauacca gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc       60

acuagacgug ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua      120

ucuuuuccaa ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac      180

augugugaug ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau      240

gacgucgauu guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac      300

aaaaaaggug aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg      360

aagcugcaaa cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu      420

agagucgaaa auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu      480

uggcuuuugg gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu      540

gccccggcau acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug      600

ucagguggga cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca      660

caggacaaac cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag      720

guaagauccu acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca      780

acacaaggug aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg      840

uuaguggaca gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca      900

ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug      960

gaguaccgga uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac     1020

acaggacaug aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga     1080

gccgaagcca cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc     1140

cuugacuuuu cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag     1200

gagugguucc acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc     1320

gugguucuag ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu     1380

gagauggaug gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug     1440

gauaaacuua gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc     1500

aagaucccgg cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca     1560

gauggaccuu gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu     1620

gggagguuga uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug     1680

cuggaacuug auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag     1740

aucacccacc acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug     1800

agaggugcca agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga     1860

ggcgcucuca acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca     1920

uuguuuggag gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug     1980

ggucugaaca caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug     2040

aucuucuuau ccacagccgu cucugcu                                         2067


<210>  906
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  906
gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1800

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1860

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1920

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     1980

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2040

uucuuaucca cagccgucuc ugcu                                            2064


<210>  907
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS wild type

<400>  907
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  908
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  908
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcggc cgucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  909
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  909
cggggcgcag auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg       60

gccgucacua gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag      120

gccauaucuu uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu      180

ggacacaugu gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa      240

ccagaugacg ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc      300

caucacaaaa aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc      360

acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac      420

uugauuagag ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc      480

aucgcuuggc uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug      540

cugauugccc cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa      600

gguaugucag gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua      660

auggcacagg acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug      720

gcggagguaa gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc      780

ugcccaacac aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa      840

agaacguuag uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug      900

gugacaugcg cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag      960

aaucuggagu accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu     1020

aaugacacag gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca     1080

ccaagagccg aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg     1140

acaggccuug acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu     1200

cacaaggagu gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa     1320

acugucgugg uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug     1380

gaggcugaga uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug     1440

aaaauggaua aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca     1500

uucaccaaga ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca     1560

gggacagaug gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc     1620

ccaguuggga gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag     1680

augaugcugg aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag     1740

aagaagauca cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc     1800

acugugagag gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca     1860

guuggaggcg cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc     1920

aaaucauugu uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug     1980

ugguuggguc ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga     2040

guguugaucu ucuuauccac agccgucucu gcu                                  2073


<210>  910
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  910
agaggauugu ccucaaggaa acgccguucc caugauguuc ugacugugca auuccuaauu       60

uugggaaugc uguugaugac ggguggagug acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgcc        2097


<210>  911
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS wild type

<400>  911
agaggauugu ccucaaggaa acgccguucc caugauguuc ugacugugca auuccuaauu       60

uugggaaugc uguugaugac gggugccgug acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgcc        2097


<210>  912
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long CDS wild type

<400>  912
agaauaauca augcuaggaa ggagaagaag agacggggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  913
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long CDS wild type

<400>  913
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca cgugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacu guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaaucuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacgc aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  914
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long CDS wild type

<400>  914
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa     1080

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaagcuua     1140

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug     1200

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauucaag     1320

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac     1380

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu     1440

ggccauuuga aaugccgccu aaaaauggac aagcuuagau ugaagggcgu gucauauucc     1500

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1560

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1620

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1680

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1740

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1800

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1860

acagccuggg acuucggauc agucgggggu guguucaacu cacuggguaa gggcauucac     1920

cagauuuuug gagcagccuu caaaucacug uuuggaggaa uguccugguu cucacagauc     1980

cucauaggca cgcugcuagu gugguuaggu uugaacacaa agaauggauc uaucucccuc     2040

acaugcuugg cccugggggg agugaugauc uuccucucca cggcuguuuc ugcugacgug     2100

gggugcucag uggacuucuc aaaa                                            2124


<210>  915
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long CDS wild type

<400>  915
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guccacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  916
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long CDS wild type

<400>  916
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacaguugac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugauu gucaaugaua caggauauga aacugacgaa     1080

aauagagcga aagucgaggu uacgccuaau ucaccaagag cggaagcaac cuugggaggc     1140

uuuggaagcu uaggacuuga cugugaacca aggacaggcc uugacuuuuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uugccuuggc augcuggggc agacaccgga acuccacacu ggaacaacaa agaggcauug     1320

guagaauuca aggaugccca cgccaagagg caaaccgucg ucguucuggg gagccaggaa     1380

ggagccguuc acacggcucu cgcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aagcuguucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac ugcggcauuc acauucacca aggucccagc ugaaacacug     1560

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagauccca     1620

guccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaacuca aagaugaugu uggagcuuga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccacca cuggcauagg     1800

agugguagca ccaucggaaa ggcauuugag gccacuguga gaggcgccaa gagaauggca     1860

guccuggggg auacagccug ggacuucgga ucagucgggg guguguucaa cucacugggu     1920

aagggcauuc accagauuuu uggagcagcc uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacgcugcua gugugguuag guuugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu ggcccugggg ggagugauga ucuuccucuc cacggcuguu     2100

ucugcugacg uggggugcuc aguggacuuc ucaaag                               2136


<210>  917
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long CDS wild type

<400>  917
agaauaauca augcuaggaa ggagaggaag agacguggcg cagacaccag caucggaauc       60

guuggccucc uguugacuac agccauggca gcagagauca cuagacgugg gagugcauac      120

uacauguacu uggauaggag cgaugcaggg aaggccauuu cuuucgcuac cacauugggg      180

gugaacaaau gccaugugca gaucauggac cucgggcaca ugugugacgc caccaugagc      240

uaugaaugcc cuaugcugga cgagggggug gaaccagaug acgucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaua aaaaagguga agcacggcga      360

ucuagaagag ccgucacgcu cccaucucac uccacaagga aauugcaaac gcggucgcag      420

acuuggcuag aaucaagaga auacacaaag caccugauca agguugaaaa uuggauauuc      480

aggaacccug guuuuacgcu aguggcuguc gccaucgccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauaggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugac      660

guuguccugg aacauggagg cugcgucacc gugauggcac aggacaagcc aacaguugac      720

auagagcugg ucacaacaac gguuaguaac auggccgagg ugagauccua uuguuacgag      780

gcaucaauau cggacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auauguuugc aaaagaacau ugguggacag agguugggga      900

aaugggugug gacucuuugg caaagggagu uuggugacau gugcuaaguu cacgugcucc      960

aagaagauga cugggaagag cauucagccg gagaaccugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcacag ugggaugauu guuaaugaug aaaacagagc gaaggucgag     1080

guuacgccca auucaccaag agcagaagca acccugggag gcuuuggaag cuuaggacuu     1140

gauugugaac caaggacagg ccuugacuuu ucagaucugu auuaccuaac caugaauaac     1200

aagcauuggu uggugcacaa agagugguuu caugacaucc cauugcccug gcaugcuggg     1260

gcagacacug gaacuccaca uuggaacaac aaggaggcau uaguggaauu caaggacgcc     1320

cacgccaaga ggcaaaccgu cgugguuuug gggagccagg aaggagccgu ccacacggcu     1380

cuugcuggag cucuagaggc ugagauggau ggugcaaagg gaaggcuauu cucuggccac     1440

uugaaauguc gcuuaaaaau ggacaagcuu agauugaagg gcgugucaua uuccuugugc     1500

accgcggcau ucacauucac caaggucccg gcugaaacac uacauggaac agucacagug     1560

gaggugcagu augcagggac agauggaccc ugcaaggucc cagcccagau ggcgguggac     1620

augcagaccu ugaccccagu cggaaggcug auaaccgcca accccgugau uacugaaagc     1680

acugagaauu caaagaugau guuggagcuc gacccaccau uuggggauuc uuacauuguc     1740

auaggaguug gggauaagaa aaucacccau cacuggcaua ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggcgcu aagagaaugg caguccuggg ggacacagcu     1860

ugggacuuug gaucagucgg agguguguuu aacucauugg gcaagggcau ucaucagauu     1920

uuuggagcag cuuucaaauc acuguuugga ggaauguccu gguucucaca gauccucaua     1980

ggcacucugc uggugugguu aggucugaac acaaagaaug ggucuaucuc ccucacaugc     2040

uuagcccugg ggggagugau gaucuuccuc uccacggcug uuucugcuga cguggggugc     2100

ucgguggacu ucucaaag                                                   2118


<210>  918
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long CDS wild type

<400>  918
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucgca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacaguugac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugauu gucaaugaua caggauauga aacugacgaa     1080

aauagagcga aagucgaggu uacgccuaau ucaccaagag cggaagcaac cuugggaggc     1140

uuuggaagcu uaggacuuga cugugaacca aggacagguc uugacuuuuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uugccuuggc augcuggggc agacaccgga acuccacacu ggaacaacaa agaggcauug     1320

guagaauuca aggaugccca cgccaagagg caaaccgucg ucguucuggg gagccaggaa     1380

ggagccguuc acacggcucu cgcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aagcuguucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac ugcggcauuc acauucacca aggucccagc ugaaacacug     1560

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagauccca     1620

guccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaacuca aagaugaugu uggagcuuga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccacca cuggcauagg     1800

agugguagca ccaucggaaa ggcauuugag gccacuguga gaggcgccaa gagaauggca     1860

guccuggggg auacagccug ggacuucgga ucagucgggg guguguucaa cucacugggu     1920

aagggcauuc accagauuuu uggagcagcc uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacgcugcua gugugguuag guuugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu ggcccugggg ggagugauga ucuuccucuc cacggcuguu     2100

ucugcugacg uggggugcuc aguggacuuc ucaaag                               2136


<210>  919
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long CDS wild type

<400>  919
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacaguugac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugacu gucaaugaua uaggauauga aacugacgaa     1080

aauagagcga aagucgaggu uacgccuaau ucaccaagag cggaagcaac cuugggaggc     1140

uuuggaagcu uaggacuuga cugugaacca aggacaggcc uugacuuuuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uugccuuggc augcuggggc agacacugga acuccacacu ggaacaacaa agaggcauug     1320

guagaauuca aggaugccca cgccaagagg caaaccgucg ucguucuggg gagccaggaa     1380

ggagccguuc acacggcucu cgcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aagcuguucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac ugcggcauuc acauucacca aggucccagc ugaaacacug     1560

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagauccca     1620

guccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaacuca aagaugaugu uggagcuuga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccacca cuggcauagg     1800

agugguagca ccaucggaaa ggcauuugag gccacuguga gaggcgccaa gagaauggca     1860

guccuggggg auacagccug ggacuucgga ucagucgggg guguguucaa cucacugggu     1920

aagggcauuc accagauuuu uggagcagcc uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacgcugcua gugugguuag guuugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu ggcccugggg ggagugauga ucuuccucuc cacggcuguu     2100

ucugcugacg uggggugcuc aguggacuuc ucaaag                               2136


<210>  920
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long CDS wild type

<400>  920
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaccggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaaa uagagcgaaa     1080

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaagcuua     1140

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug     1200

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauucaag     1320

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac     1380

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaaa gcuguucucu     1440

ggccauuuga aaugccgccu aaaaauggac aagcuuagau ugaagggcgu gucauauucc     1500

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1560

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1620

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1680

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1740

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1800

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1860

acagccuggg acuucggauc agucgggggu guguucaacu cacuggguaa gggcauucac     1920

cagauuuuug gagcagccuu caaaucacug uuuggaggaa uguccugguu cucacagauc     1980

cucauaggca cgcugcuagu gugguuaggu uugaacacaa agaauggauc uaucucccuc     2040

acaugcuugg cccugggggg agugaugauc uuccucucca cggcuguuuc ugcugacgug     2100

gggugcucag uggacuucuc aaag                                            2124


<210>  921
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long CDS wild type

<400>  921
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aggggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  922
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long CDS wild type

<400>  922
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagaugcuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  923
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long CDS wild type

<400>  923
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagaa agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gccucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  924
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long CDS wild type

<400>  924
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc uacugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cugguacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  925
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long CDS wild type

<400>  925
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggaga uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guggaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcugcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  926
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long CDS wild type

<400>  926
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucua     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  927
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long CDS wild type

<400>  927
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuacgcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccagcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg ucacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagua ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  928
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long CDS wild type

<400>  928
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacug auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacuccu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagucguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  929
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long CDS wild type

<400>  929
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucag cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggccgcaa      420

accugguugg aaucaagaga auacacaaag cauuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaaca      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  930
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long CDS wild type

<400>  930
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aaguucaacg      540

agcaaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg gacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  931
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long CDS wild type

<400>  931
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

cgccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacug auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacuccu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  932
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long CDS wild type

<400>  932
agaauaauca augcuaggaa ggagaagaag agacgaagcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc cacuaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaauaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacac gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  933
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long CDS wild type

<400>  933
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuaa ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

acuugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucc aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccag gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  934
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long CDS wild type

<400>  934
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagaaacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag ccgugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug guuucgcuuu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aauguuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  935
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long CDS wild type

<400>  935
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccaccagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cauuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaaca      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagccucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucugacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  936
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long CDS wild type

<400>  936
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuaa ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa cuggauguuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguccca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  937
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long CDS wild type

<400>  937
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugagugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauacuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauauuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacucag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggaaucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  938
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long CDS wild type

<400>  938
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuucaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaacaauau cagacauagc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugccg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  939
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long CDS wild type

<400>  939
agaauaauca augccaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauc       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggggg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcu uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gacucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  940
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long CDS wild type

<400>  940
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccaccagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucugacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga aaguugagau aacgcccaau ucaccgagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga guuacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  941
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long CDS wild type

<400>  941
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagaaacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggauagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag ccgugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gaaucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gauaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcccagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  942
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long CDS wild type

<400>  942
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcgcu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua uagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac cuuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcau ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gugcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucaucgg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  943
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long CDS wild type

<400>  943
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg uguaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aaaaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuuaggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccu uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  944
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long CDS wild type

<400>  944
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uacauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgccu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aagcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc ucaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  945
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long CDS wild type

<400>  945
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uacauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcu uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggaagcaca ugcuaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc ucaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  946
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long CDS wild type

<400>  946
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca ccagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu ugguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcauugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcaggggcag auggacccug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  947
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long CDS wild type

<400>  947
agaauaauca augcugggaa ggagaagaag agacgaggcg cagauacuaa ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

acuugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acguucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggaggcugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucc aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccag gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  948
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long CDS wild type

<400>  948
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagaaacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa agaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag ccgugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug guuucgcuuu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auauguuugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aauguuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcauagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  949
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long CDS wild type

<400>  949
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcagagguca cuagacgugg gagugcauac      120

uacauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggu uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuucuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc ucaugugcuu ggccuuaggg ggagugcuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  950
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long CDS wild type

<400>  950
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuaa ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

augacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugaccu gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcagucc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccuguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

agugguagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaacgga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  951
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long CDS wild type

<400>  951
agaauaauca augccaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugcgaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaau      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccgc ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccuguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa cucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  952
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long CDS wild type

<400>  952
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagaaacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag ccgugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug guuucgcuuu agcagcagcu gccaucgcgu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug cccuggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aauguuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcccagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  953
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long CDS wild type

<400>  953
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uacauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccauagcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggaa ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca guaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaaucc uaggacugga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc ucaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  954
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long CDS wild type

<400>  954
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggaaguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca cgugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccauuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

caugggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacgc aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  955
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long CDS wild type

<400>  955
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uacauguacu uggacagaaa cgaugcuggg gaggccauau cuuucccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacagaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag uuauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg cugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacaccca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacugga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag auacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc ucaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  956
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long CDS wild type

<400>  956
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugccggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaagc gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua uagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacuguugac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc uuggugacau gcgcuaaguu ugcaugcucc      960

aagaagauga ccgggaagag cauccagcca gagaauuugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aacagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaaag agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gugcucuuaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  957
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long CDS wild type

<400>  957
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaag cgaugcuggg gaggccauau cuuuuccaac cacacugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggua gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcag      420

accugguugg agucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagauaccca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg aaaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guccauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guccacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg acaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggacccug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagua ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gccugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  958
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long CDS wild type

<400>  958
agaauaauca augcuaggaa ggagaagaag agacgaggca cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaag cgaugcuggg gaggccauau cuuuuccaac cacacugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggua gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

uccagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucaguaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaacaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acacuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaggcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcguucuuaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaauac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

uccgcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  959
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long CDS wild type

<400>  959
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu cccuucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc gcaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auauguuugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugcuc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug gcucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agccaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacagug     1560

gacgggacag ucacagugga gggacaguac ggagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcagacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuau uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg gacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucagguggug uggggugcuc gguggacuuc ucaaag                               2136


<210>  960
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long CDS wild type

<400>  960
agaauaauca augcuaggaa ggagaagaag agacgaggca cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agccauggca gcggaaguca cuagacgugg gagugcauac      120

uauauguacu uggacagaag cgaugcuggg gaggccauau cuuuuccaac cacacugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggua gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaccaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu ggcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucucca agaucccggc ugaaacacug     1560

cacgggacag ucacagagga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccgguaauca cugaaagcac agagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaauac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  961
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long CDS wild type

<400>  961
agaauaauca augccaggaa ggagaagaag agacgaggca cagacacuag ugucggaauu       60

guuggccucc ugcugaccac agccauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaag cgaugcuggg gaggccauau cuuuuccaac cacacugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggua gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaccaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccac uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcucuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug cuccggcaua uagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaaggcga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgccaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccgaau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccugggaggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaauuca aggaugcaca ugccaaaagg caaacugucg ugguucuagg gagucaagag     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu cccuguguac cgcagcguuc acauucacua agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccuguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaauac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  962
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long CDS wild type

<400>  962
agaauaauca augcuaggaa ggagaagaag agacgaggca cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agccauggca guggagguca cuagacgugg gaaugcauac      120

uauauguacu uggacagaag cgaugcuggg gaggccauau cuuuuccaac cacaaugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggua gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaccaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auacacaaag caccugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uuguguuacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcuugcucu      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaua caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc augcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg cagacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacua agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccuguaauca cugaaagcac ugagaacucc aagaugaugc uggaacugga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gaaaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguugggg gugcucucaa cucacugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug gugugguugg gucugaauac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  963
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long CDS wild type

<400>  963
agaauaauca augcuaggaa ggaaaagaag agacgaggca cagacacuag ugucggaauu       60

guuggccucc ugcugaccac agccauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaag cgaugcuggg gaggccauau cuuuuccaac cacacugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggua gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaccaca aaaaagguga agcgcggaga      360

ucuagaagag cugugacgcu ccccucccac uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu ggcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua uagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

gucgucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcagagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgccaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccgaau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccugggaggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggaugcaca ugccaaaagg caaacugucg ugguccuagg gagucaagag     1380

ggagcaguuc acacggcucu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag acugaagggc     1500

gugucauacu cccuguguac cgcagcguuc acauucacua agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccuguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aaggguaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaauac aaagaaugga     2040

ucuauuuccc uugugugcuu ggccuuaggg ggaguguuga ucuucuuguc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  964
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long CDS wild type

<400>  964
agaauaauca augcuaggaa ggagaagaag agacgaggca cagauacuag ugucggaauu       60

guuggccucc ugcugaccac agccauggca guggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaag cgaugcuggg gaggccauau cuuuuccaac cacacugggg      180

augaacaagu guuacauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuauguugga ugagggggua gaaccagaug acgucgauug uuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugccaccaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auauacaaag caccugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgccu ggcuuuuggg aaguucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uuguguuacc guaauggcac aggacaaacc ggcugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua uugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcagu cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag cugaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc augcuggggc agacaccgga acuccacauu ggaacaacaa agaagcauug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggacccug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggaggcugau aaccgcuaac     1680

ccuguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccauca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggauuuugga ucaguuggag gugcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug gugugguugg gucugaauac aaagaaugga     2040

ucuauuuccc uuacgugcuu ggccuuaggg ggaguguuga ucuuuuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  965
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long CDS wild type

<400>  965
agaauaauca augcuaggaa ggagaagaag agacgaggcg cagauacuag cgucggaauu       60

guuggccucc uccugaccac agccauggca guagagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaag cgaugcuggg gaggccauau cuuuuccaac cacacugggg      180

augaauaagu guuacauaca aaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuauguugga ugagggggua gaaccagaug acgucgauug cuggugcaac      300

acgacaucaa cuuggguugu guauggaacc ugccaccaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auacacaaag caccugauua gaguugaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gucaucgcuu ggcuuuuggg aaguucaacg      540

agccaaaaag ucauauaucu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

guugucuugg aacauggagg uuguguuacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cggauauggc uucggacagc cgcugcccaa cacaagguga ggccuaccuu      840

gacaagcagu cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuugggagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uaccugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc augcuggggc agacacugga acuccacauu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugcaaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg agccaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuugugcac ugcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggauau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccuguaauca cugaaagcac cgagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccauca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguugggg gugcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguucggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug gugugguugg gucugaauac aaagaaugga     2040

ucuauuuccc uuacgugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguu     2100

ucugcugaug uggggugcuc gguggacuuc ucaaag                               2136


<210>  966
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long CDS wild type

<400>  966
agaauaauca augcuaggaa ggagaagaag agacguggcg cagacaccag ugucggaauu       60

guuggccucc ugcugaccac agccauggca guggagguca ccagacgugg gagugcauac      120

uauauguacu uagacagaag cgaugcuggg gaggccauau cuuuuccaac cacacugggg      180

gugaauaagu guuacauaca gaucauggau cuuggacaca ugugugaugc cacaaugagc      240

uaugaaugcc cuauguugga ugagggggua gaaccagaug acgucgauug cuggugcaac      300

acgacaucga cuuggguugu guacggaacc ugccaucaca aaaaagguga ggcacggaga      360

ucuagaagag cugugacgcu ccccucucau uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auacacaaag cacuugauca gagucgaaaa uuggauauuc      480

aggaacccug gcuuugcguu ggcagcagcu gccauugcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cuguugauug ccccggcaua caguaucagg      600

ugcauaggag ucagcaauag ggauuuugug gaagguaugu caggugggac cuggguugau      660

guugucuugg aacauggagg uuguguuacc guaauggcac aggacaagcc aacuguugau      720

auagaguugg ucacaacaac gguuagcaac auggcggagg uaagauccua cugcuacgag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcagu cagacacuca auauguuugc aaaagaacgu uaguggacag agguugggga      900

aauggaugug gacucuuugg caaagggagc cuggugacau gcgccaaguu ugcaugcucc      960

aagaaaauga cugggaagag cauccagcca gagaaccugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauu guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccugggaggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcauugguug gugcacaagg agugguucca ugacauucca     1260

cuaccuuggc augcuggggc agacaccgga acuccacauu ggaacaacaa agaagcauug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagccguuc acacggcucu ugcuggagcc cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc uugaaaaugg acaaacuuag auugaagggc     1500

gugucauacu ccuuauguac cgcggcguuc acauucacca agaucccggc ugaaacgcug     1560

caugggacag ucacagugga gguacaguau gcagggacag auggacccug caagguucca     1620

gcucagaugg cgguggauau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccugugauca cugaaagcac ugagaauuca aagaugaugu uggaacuuga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gauaagaaga ucacccacca cugguacagg     1800

aguggcagca ccaucggaaa agcauuugaa gccacuguga gaggcgccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucagucggag gugcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauagg aacguugcug gugugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuacgugcuu ggccuuaggg ggaguguuga ucuuccuauc uacagccguc     2100

ucugcugaug ugggguguuc gguggacuuc ucaaag                               2136


<210>  967
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long CDS wild type

<400>  967
agaauaauca augcuaggaa ggagaagaag agacguggcg cagacaccag ugucggaauu       60

guuggccucc ugcugaccac agccauggca guggagguca ccagacgugg gagugcauac      120

uauauguacu uagacagaag cgaugcuggg aaggccauau cuuuuccaac cacacugggg      180

gugaauaagu guuacauaca gaucauggau cuuggacaca ugugugaugc cacaaugagc      240

uaugaaugcc cuauguugga ugagggggua gaaccagaug acgucgauug cuggugcaac      300

acgacaucga cuuggguugu guacggaacc ugccaucaca aaaaagguga ggcacggaga      360

ucuagaagag cugugacgcu ccccucucau uccacuagga agcugcaaac gcggucgcag      420

accugguugg aaucaagaga auacacaaag cacuugauca gagucgaaaa uuggauauuc      480

aggaacccug gcuuugcguu ggcagcagcu gccauugcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cuguugauug ccccggcaua caguaucagg      600

ugcauaggag ucagcaauag ggauuuugug gaagguaugu caggugggac cuggguugau      660

guugucuugg aacauggagg uuguguuacc guaauggcac aggacaagcc aacuguugau      720

auagaguugg ucacaacaac gguuagcaac auggcggagg uaagauccua cugcuacgag      780

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcagu cagacacuca auauguuugc aaaagaacgu uaguggacag agguugggga      900

aauggaugug gacucuuugg caaagggagc cuggugacau gcgccaaguu ugcaugcucc      960

aagaaaauga cugggaagag cauccagcca gagaaccugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauu guuaaugaca uaggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccugggaggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcauugguug gugcacaagg agugguucca ugacauucca     1260

cuaccuuggc augcuggggc agacaccgga acuccacauu ggaacaacaa agaagcauug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagccguuc acacggcucu ugcuggagcc cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc uugaaaaugg acaaacuuag auugaagggc     1500

gugucauacu ccuuauguac cgcggcguuc acauucacca agaucccggc ugaaacgcug     1560

caugggacag ucacagugga gguacaguau gcagggacag auggacccug caagguucca     1620

gcucagaugg cgguggauau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

ccugugauca cugaaagcac ugagaauuca aagaugaugu uggaacuuga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gauaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccaucggaaa agcauuugaa gccacuguga gaggcgccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucagucggag gugcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uccucauagg aacguugcug gugugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuacgugcuu ggccuuaggg ggaguguuga ucuuccuauc uacagccguc     2100

ucugcugaug ugggguguuc gguggacuuc ucaaag                               2136


<210>  968
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long CDS wild type

<400>  968
agaauaauca augcuaggaa ggagaggaag agacguggcg cagacaccag caucggaauc       60

guuggccucc uguugacuac agccauggca gcagagauca cuagacgugg gagugcauac      120

uacauguacu uggauaggag cgaugcaggg aaggccauuu cuuucgcuac cacauugggg      180

gugaacaaau gccaugugca gaucauggac cucgggcaca ugugugacgc caccaugagc      240

uaugaaugcc cuaugcugga cgagggggug gaaccagaug acgucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaua aaaaagguga agcacggcga      360

ucuagaagag ccgucacgcu cccaucucac uccacaagga aauugcaaac gcggucgcag      420

acuuggcuag aaucaagaga auacacaaag caccugauca agguugaaaa uuggauauuc      480

aggaacccug guuuuacgcu aguggcuguc gccaucgccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauaggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugac      660

guuguccugg aacauggagg cugcgucacc gugauggcac aggacaagcc aacaguugac      720

auagagcugg ucacaacaac gguuaguaac auggccgagg ugagauccua uuguuacgag      780

gcaucaauau cggacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auauguuugc aaaagaacau ugguggacag agguugggga      900

aaugggugug gacucuuugg caaagggagu uuggugacau gugcuaaguu cacgugcucc      960

aagaagauga cugggaagag cauucagccg gagaaccugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcacag ugggaugauu guuaaugaca caggacauga aacugaugaa     1080

aacagagcga aggucgaggu uacgcccaau ucaccaagag cagaagcaac ccugggaggc     1140

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau     1200

uaccuaacca ugaauaacaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uugcccuggc augcuggggc agacacugga acuccacauu ggaacaacaa ggaggcauua     1320

guggaauuca aggacgccca cgccaagagg caaaccgucg ugguuuuggg gagccaggaa     1380

ggagccgucc acacggcucu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aggcuauucu cuggccacuu gaaaugucgc uuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac cgcggcauuc acauucacca aggucccggc ugaaacacua     1560

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1620

gcccagaugg cgguggacau gcagaccuug accccagucg gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gauaagaaaa ucacccauca cuggcauagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggcgcuaa gagaauggca     1860

guccuggggg acacagcuug ggacuuugga ucagucggag guguguuuaa cucauugggc     1920

aagggcauuc aucagauuuu uggagcagcu uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacucugcug gugugguuag gucugaacac aaagaauggg     2040

ucuaucuccc ucacaugcuu agcccugggg ggagugauga ucuuccucuc cacggcuguu     2100

ucugcugacg uggggugcuc gguggacuuc ucaaag                               2136


<210>  969
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long CDS wild type

<400>  969
agaauaauca augcuaggaa ggagaggaag agacguggug cagacaccag caucggaauc       60

auuggucucc ugcugacuac agccauggca gcggagauua ccagacgugg aagugcauac      120

uacauguacu uggacaggag cgaugcuggg aaggccaucu ccuuugcuac caacuuggga      180

guuaacaaau gccauguaca gaucauggau cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuauguugga cgagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga agcacggcga      360

ucuagaagag cugugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag caccugauca agguugaaaa cuggauauuu      480

aggaaccccg gguuugcgcu cgcagcuguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu gguuaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauuggag ucagcaauag agacuucgug gaaggcaugu caggugggac cuggguugau      660

gucgucuugg aacauggagg uugcgucacc guuauggcac aggacaagcc aacaguugac      720

auagaguugg ucacgacaac gguuaguaac auggccgagg uaagauccua cuguuaugag      780

gcaucaauau cagacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      840

gauaagcaau cagacaccca auaugugugc aaaagaacau ugguggacag agguugggga      900

aaugggugug gacuuuuugg caaagggagc cuggugacau gugccaaguu cacguguucc      960

aagaagauga cugggaagag cauucagccg gagaaucugg aguaucgaau aaugcuauca     1020

gugcauggcu cccagcacag cgggaugauu guuaaugaca uaggacauga aacugacgaa     1080

aacagagcga aagucgaggu cacgcccaau ucaccaagag cagaagcaac cuugggaggc     1140

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uggacuuuuc agauuuguac     1200

uacuugacca ugaacaacaa gcacugguug gugcacaaag agugguuuca ugacauccca     1260

uugccuuggc augcuggggc agauaccgga acuccacacu ggaacaacaa ggaggcauug     1320

guagaguuca aagaugccca cgccaagagg caaaccgucg uuguucuagg gagccaggaa     1380

ggagccguuc acacggcucu ugcuggagcu cuggaggcug agauggaugg ugcaaaagga     1440

aggcuguucu ccggccauuu gaaaugccgc uuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac cgcagcguuc acauuuacca aggucccagc ugaaacauug     1560

cauggaacag uuacagugga ggugcaguau gcagggacag auggacccug caagguccca     1620

gcccagaugg cgguggacau gcagacccug accccagucg ggaggcugau aaccgccaac     1680

ccugugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccauca cuggcauagg     1800

agugguagca ccaucggaaa agcauuugag gccacuguga gaggugccaa gagaauggca     1860

guucuggggg auacagccug ggacuucgga ucagucgggg gcguguucaa cucauugggc     1920

aaggguguuc accagauuuu uggagcagcu uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacgcugcug gugugguugg guuugaacac aaagaaugga     2040

uccaucuccc ucacaugcuu ggcccugggg ggagugauga ucuuccucuc cacggcuguc     2100

ucugcugacg uggggugcuc gguggacuuc ucaaag                               2136


<210>  970
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long CDS wild type

<400>  970
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggcga      360

ucuagaagag ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      420

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacaguugac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aaugguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu cacguguucu      960

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca     1020

gugcauggcu cccagcauag cgggaugauu gucaaugaua uaggacauga aacugacgaa     1080

aacagagcga aagucgaggu uacgccuaau ucaccaagag cggaagcaac cuugggaggc     1140

uuuggaagcu uaggacuuga cugugaacca aggacaggcc uugacuuuuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uugccuuggc augcuggggc agacaccgga acuccacacu ggaacaacaa agaggcauug     1320

guagaauuca aggaugccca cgccaagagg caaaccgucg ucguucuggg gagccaggaa     1380

ggagccguuc acacggcucu cgcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aggcuguucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac ugcggcauuc acauucacca aggucccagc ugaaacacug     1560

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1620

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaacuca aagaugaugu uggagcuuga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccacca cuggcauagg     1800

agugguagca ccaucggaaa ggcauuugaa gccacuguga gaggcgccaa gagaauggca     1860

guccuggggg auacagccug ggacuucgga ucagucgggg guguguucaa cucacugggu     1920

aagggcauuc accagauuuu uggagcagcc uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacgcugcua gugugguuag guuugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu ggcccugggg ggagugauga ucuuccucuc cacggcuguu     2100

ucugcugacg uggggugcuc aguggacuuc ucaaag                               2136


<210>  971
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long CDS wild type

<400>  971
agaauaauca augcuaggaa ggagaggaag agacguggug cagacaccag caucggaauc       60

auuggucucc ugcugacuac agccauggca gcagagauua ccagacgugg aagugcauac      120

uacauguacu uggacaggag cgaugcuggg aaggccaucu ccuuugcuac caauuuggga      180

guuaacaaau gccauguaca gaucauggau cucgggcaca ugugugacgc caccaugagu      240

uaugaguguc cuauguugga cgagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga agcacggcga      360

ucuagaagag cugugacgcu cccuucucac ucuacaagga agcugcaaac gcggucgcag      420

acuugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      480

aggaaccccg gguuugcgcu cgcagcuguu gccauugccu ggcuuuuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauuggag ucagcaauag agacuucgug gaaggcaugu caggugggac cuggguugau      660

gucgucuugg aacauggagg uugcgucacc guaauggcac aggacaagcc aacaguugac      720

auagaguugg ucacgacaac ggucaguaac auggccgagg uaagauccua cuguuaugag      780

gcaucaauau cggacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auauguaugc aaaagaacau ugguggacag agguugggga      900

aaugggugug gacuuuuugg caaagggagc cuggugacau gugccaaguu cacguguucc      960

aagaagauga ccgggaagag cauucagccg gagaaucugg aguaucgaau aaugcuauca     1020

gugcauggcu cccagcacag cgggaugauu guuaaugacg aaaauagggc gaaagucgag     1080

gucacgccca auucaccaag agcagaagca accuugggag gcuuuggaag cuuaggacuu     1140

gauugugaac caaggacagg ccuggacuuu ucagauuugu acuacuugac caugaacaau     1200

aagcacuggu uagugcacaa agagugguuu caugacaucc cauugcccug gcaugcuggg     1260

gcagacaccg gaacuccaca uuggaacaac aaggaggcau ugguagaguu caaagaugcc     1320

cacgcuaaga ggcaaaccgu cguuguucua gggagccagg aaggagccgu ucacacggcu     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaaag gaagguuguu cuccggccau     1440

uugaaaugcc gcuuaaaaau ggacaagcuu agauugaagg gcgugucaua uuccuugugc     1500

accgcagcgu ucacauucac caagguccca gcugaaacau ugcauggaac aguuacagug     1560

gaggugcagu augcagggac agauggaccc ugcaaggucc cagcucagau ggcgguggac     1620

augcagaccc ugaccccagu cggaaggcug auaaccgcca accccgugau uacugaaagc     1680

acugagaauu caaagaugau guuggagcuc gacccaccau uuggggauuc uuacauuguc     1740

auaggaguug gggacaagaa aauuacccac cacuggcaua ggagugguag caccaucgga     1800

aaagcauuug aggccacugu gagaggugcc aagagaaugg caguucuggg ggacacagcc     1860

ugggacuuug gaucagucgg gggcguguuu aacucauugg gcaagggcau ccaccagauu     1920

uuuggagcag ccuucaaauc acuguuugga ggaauguccu gguucucaca gauccucaua     1980

ggcacgcugc uggugugguu ggguuugaac acaaagaaug gauccaucuc ccucacaugc     2040

uuggcccugg ggggagugau gaucuuccuc uccacggcug ucucugcuga cguggggugc     2100

ucgguggacu ucucaaag                                                   2118


<210>  972
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long CDS wild type

<400>  972
agaauuauca augcuaggaa ggagaggaag agacguggcg cugacaccag caucggaauc       60

gucggccucc ugcugaccac agccauggca gccgagauca cuagacgugg gagugcauac      120

uacauguacu uggacaggag cgaugcuggu aaggccauuu cuuuugccac cacauugggg      180

gugaacaaau gccauguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc ccaugcuaga cgagggagug gagccagaug acgucgauug cuggugcaac      300

acgacaucga cuuggguugu guacggaacc ugucaucaua aaaaagguga agcacgacga      360

uccagaagag ccgugacgcu uccuucucac uccacgagga agcugcaaac gcgaucgcag      420

acuuggcuag agucaagaga auacacaaag caccugauca agguugaaaa uuggauauuc      480

aggaaccccg gguuugcgcu aguggcugua gcuauagccu ggcuccuggg aagcucgacg      540

agccaaaaag uuauauacuu ggucaugaua uuguugauug ccccggcaua cagcauuagg      600

ugcauaggag uuagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggggg uugcgucacc gugauggcac aggacaagcc aacaguugau      720

aucgaguugg ucacgacaac gguuagcaac auggccgagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cguuguccaa cacaagguga agccuaccuu      840

gacaagcagu cagacacuca auaugucugc aagagaacau ugguggauag agguugggga      900

aaugggugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacgugcucc      960

aagaaaauga caggcaagag cauccagccg gagaacuugg aguaccggau aaugcuauca     1020

gugcauggau cccagcacag ugggaugauu gugaaugaca caggacauga aacugacgaa     1080

aacagagcaa aagucgaggu cacacccaau ucaccaagag cagaagcaac cuugggaggu     1140

uuuggaagcu ugggacuuga cugugaacca aggacaggcc uugacuucuc agaucuguau     1200

uacuugacca ugaacaauaa gcauugguug gugcacaagg agugguuuca ugacauccca     1260

uuaccuuggc augcuggugc agacacugga acuccacacu ggaacaacaa agaggcauug     1320

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagag     1380

ggagcuguuc auacggcccu cgcuggagcu uuggaggcug agauggaugg ugcaaaggga     1440

aggcuauucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu     1500

gugucauauu cccuguguac cgcagcguuc acauuuacca aggucccagc ugaaacauug     1560

cauggaacag ucacagugga ggugcaguau gcagggacag acggacccug caaaguccca     1620

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaau     1680

ccugugauca cugaaaguac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1800

agugguagca ccaucggaaa ggcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuuggggg acacagccug ggacuuugga ucaguugggg guguguuuaa uucauugggu     1920

aaggguauuc accagaucuu uggagcagcu uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacacuguug gugugguuag guuugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu agcccugggg ggagugauga ucuuccuuuc cacggcuguu     2100

ucugcugaug uugggugcuc gguggacuuc ucaaag                               2136


<210>  973
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long CDS wild type

<400>  973
agaauuauca augcuaggaa ggagaggaag agacguggcg cugacaccag caucggaauc       60

gucggccucc ugcugaccac agccauggca gccgagauca ccagacgugg gagugcauac      120

uacauguacu uggacaggag cgaugcuggu aaggccauuu cuuuugcuac cacauugggg      180

gugaacaaau gccauguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc ccaugcugga cgagggagug gagccagaug acgucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaacaua aaaaagguga agcacgacga      360

uccagaagag ccgugacgcu uccuucucac uccacaagga agcugcaaac gcgaucacag      420

acuuggcuag aaucaagaga auacacaaag caccugauca agguugagaa uuggauauuc      480

aggaaccccg gguuugcgcu aguggcugua gcuauugccu ggcuccuggg aagcucgacg      540

agccaaaaag ucauauacuu gguaaugaua uuguugauug ccccggcaua cagcaucagg      600

ugcauaggag uuagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggggg uugcgucacc gugauggcac aggacaagcc aacaguugac      720

aucgaguugg ucacgacaac gguuagcaac auggccgagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cgcuguccaa cacaagguga agccuaccuu      840

gacaagcagu cagacacuca auaugucugc aagagaacau ugguggauag agguugggga      900

aaugggugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacgugcucc      960

aagaaaauga caggcaagag cauccagccg gagaacuugg aguaccggau aaugcuauca     1020

gugcauggau cccagcacag ugggaugauu gugaaugaca uaggacauga aacugacgaa     1080

aacagagcaa aagucgaggu cacacccaau ucaccaagag cagaagcaac cuugggaggu     1140

uuuggaagcu ugggacuuga cugugaacca aggacaggcc uugacuucuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaagg agugguuuca ugacauccca     1260

uuaccuuggc augcuggugc agacacugga acuccacacu ggaacaacaa agaggcauug     1320

guggaguuua aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagag     1380

ggagcuguuc acacggcccu cgcuggagcu uuggaggcug agauggaugg ugcaaaggga     1440

aggcuauucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu     1500

gugucauauu cccuguguac cgcagugugu accgcagcga aggucccagc ugaaacauug     1560

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1620

gcccagaugg cgguagacau gcagacccug accccaguug gaaggcugau aaccgccaac     1680

ccugugauca cugaaaguac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1800

agugguagca ccaucggaaa ggcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuuggggg acacagccug ggacuuugga ucaguugggg guguguuuaa uucauugggu     1920

aaggguauuc accagaucuu uggagcagcu uuuaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacacuguug gugugguuag gucugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu agcccugggg ggagugauga ucuuccuuuc cacggcuguu     2100

ucugcugaug uugggugcuc gguggacuuc ucaaag                               2136


<210>  974
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long CDS wild type

<400>  974
agaauaauca augcuaggaa ggagaggaag agacguggag cugaugccag caucggaauc       60

gucagccucc ugcugacuac agucauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggacaggag cgaugcuggu aaggccauuu cuuucguuac cacacugggg      180

gugaacaaau gccaugugca gaucauggac cucgggcaua ugugugacgc caccaugagu      240

uaugagugcc ccaugcugga cgagggagug gagccagaug acgucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaua aaaaagguga agcacgacga      360

uccagaagag ccgugacgcu uccuucucac ucuacaagga aguugcaaac gcgaucgcag      420

acuuggcuag aaucaagaga auacacaaag caccugauca agguugagaa uuggauauuc      480

aggaaccccg gguuugcgcu aguggcugua gcuauugccu ggcuccuggg aagcucgacg      540

agccaaaaag ucauauacuu ggucaugaua uuguugauug ccccggcaua caguaucagg      600

ugcauaggag uuagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg uugugucacc gugauggcac aggacaagcc aacaguugac      720

auagaguugg ucacgacaac gguuagcaac auggccgagg ugagauccua cugcuacgag      780

gcaucaauau cggacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      840

gacaagcagu cagacacuca auaugucugu aaaagaacau ugguggacag agguugggga      900

aaugggugug gacuuuuugg caaggggagc uuggugacgu gugccaaguu uacaugcucc      960

aagaaaauga cagggaagag cauccagccg gagaacuugg aguaccggau aaugcuauca     1020

gugcauggau cccagcacag ugggaugauu gugaaugacg aaaacagagc aaaagucgag     1080

guuacaccca auucaccaag agcagaagca accuugggag guuuuggaag ccugggacuu     1140

gauugugaac caaggacagg ccuugacuuu ucagaucugu auuaccugac caugaacaau     1200

aagcauuggu uggugcacaa agagugguuu caugacaucc cauuaccuug gcauucuggu     1260

gcagacacug aaacuccaca cuggaacaac aaagaggcac ugguggaguu caaggacgcc     1320

cacgccaaga ggcaaacugu ugugguucug gggagccaag aaggagccgu ucacacggcu     1380

cucgcuggag cucuggaggc ugagauggau ggugcgaagg gaaggcuauc cucaggccau     1440

uugaaaugcc gccuaaaaau ggacaagcuu agguugaagg gugugucaua uucccugugu     1500

accgcagcgu ucacauucac caagguucca gcugaaacau ugcauggaac agucacagug     1560

gaggugcagu augcagggag ggauggaccc ugcaaggucc cagcccagau ggcgguggac     1620

augcagaccc ugaccccagu uggaaggcug auaacggcua acccugugau cacugaaagc     1680

acugagaauu caaagaugau guuggagcuc gacccaccau uuggggauuc uuacauuguc     1740

auaggagucg gggacaagaa aaucacccau cacuggcauc ggagugguag caucaucgga     1800

aaggcauuug aagccacugu gagaggcgcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg ggguguguuu aacucauugg gcaaggguau ucaccagauc     1920

uuuggagcag cuuucaaauc acuguucgga ggaauguccu gguucucaca gauccucaua     1980

ggcacacugu uggugugguu gggucugaac acaaagaaug gaucuaucuc ccucacaugc     2040

uuggccuugg gaggagugau gaucuuccuu uccacggcug uuucugcuga uguggggugu     2100

ucgguggacu ucucaaag                                                   2118


<210>  975
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long CDS wild type

<400>  975
agaauuauca augcuaggaa ggagaggaag agacguggcg cugacaccag caucggaauc       60

gucggccucc ugcugaccac agccauggca gccgagauca cuagacgugg gagugcauac      120

uacauguacu uggacaggag cgaugcuggu aaggccauuu cuuuugccac cacauugggg      180

gugaacaaau gccauguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc ccaugcuaga cgagggagug gagccagaug acgucgauug cuggugcaac      300

acgacaucga cuuggguugu guacggaacc ugucaucaua aaaaagguga agcacgacga      360

uccagaagag ccgugacgcu uccuucucac uccacgagga agcugcaaac gcgaucgcag      420

acuuggcuag agucaagaga auacacaaag caccugauca agguugaaaa uuggauauuc      480

aggaaccccg gguuugcgcu aguggcugua gcuauagccu ggcuccuggg aagcucgacg      540

agccaaaaag uuauauacuu ggucaugaua uuguugauug ccccggcaua cagcauuagg      600

ugcauaggag uuagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggggg uugcgucacc gugauggcac aggacaagcc aacaguugau      720

aucgaguugg ucacgacaac gguuagcaac auggccgagg uaagauccua cugcuaugag      780

gcaucaauau cggacauggc uucggacagc cguuguccaa cacaagguga agccuaccuu      840

gacaagcagu cagacacuca auaugucugc aagagaacau ugguggauag agguugggga      900

aaugggugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacgugcucc      960

aagaaaauga caggcaagag cauccagccg gagaacuugg aguaccggau aaugcuauca     1020

gugcauggau cccagcacag ugggaugauu gugaaugacg aaaacagagc aaaagucgag     1080

gucacaccca auucaccaag agcagaagca accuugggag guuuuggaag cuugggacuu     1140

gacugugaac caaggacagg ccuugacuuc ucagaucugu auuaccugac caugaacaau     1200

aagcauuggu uggugcacaa ggagugguuu caugacaucc cauuaccuug gcaugcuggu     1260

gcagacacug gaacuccaca cuggaacaac aaagaggcau ugguggaguu caaggacgcc     1320

cacgccaaga ggcaaacugu ugugguucug gggagccaag agggagcugu ucauacggcc     1380

cucgcuggag cuuuggaggc ugagauggau ggugcaaagg gaaggcuauu cucuggccau     1440

uugaaaugcc gccuaaaaau ggacaagcuu agguugaagg gugugucaua uucccugugu     1500

accgcagcgu ucacauuuac caagguccca gcugaaacau ugcauggaac agucacagug     1560

gaggugcagu augcagggac agacggaccc ugcaaagucc cagcccagau ggcgguggac     1620

augcagaccc ugaccccagu uggaaggcug auaaccgcca auccugugau cacugaaagu     1680

acugagaauu caaagaugau guuggagcuc gacccaccau uuggggauuc uuacauuguc     1740

auaggagucg gggacaagaa aaucacccau cacuggcauc ggagugguag caccaucgga     1800

aaggcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg ggacacagcc     1860

ugggacuuug gaucaguugg ggguguguuu aauucauugg guaaggguau ucaccagauc     1920

uuuggagcag cuuucaaauc acuguuugga ggaauguccu gguucucaca gauccucaua     1980

ggcacacugu uggugugguu agguuugaac acaaagaaug gaucuaucuc ccucacaugc     2040

uuagcccugg ggggagugau gaucuuccuu uccacggcug uuucugcuga uguugggugc     2100

ucgguggacu ucucaaag                                                   2118


<210>  976
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long CDS wild type

<400>  976
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacauuggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga gacacggcga      360

ucuagaagau cugugucgcu ccguuaucac uauacaagga aguugcaaac gcggucgcag      420

acaugguuag aaucaagaga auacaagaag cacuugauca uggucgaaaa cuggauauuc      480

aggaaccccg gguuugccau aguguccguu gccauuaccu ggcugauggg aagcuugacg      540

agccaaaaag ucauauacuu ggucaugaua guguugauug ucccggcaua caguaucagc      600

ugcauuggag ucagcaauag agacuuagug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg gugcguuacc gagauggcac aggacaagcc aacaguugac      720

auagaguugg ucacgaugac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucguuau ccgacauggc uucggccagu cguugcccaa cacaaggcga acccucccuc      840

gacaagcaau cagacacuca aucuguaugc aaaagaacau uaggagacag agguugggga      900

aaugguugug ggauuuuugg caaagggagc uuggugacau guuccaaguu cacguguugu      960

aagaagaugc ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuccca     1020

gugcauggcu cccagcauag cgggaugauu gugaaugaca uaggacauga aacugacgaa     1080

aacagagcga aagucgaggu cacacccaau ucaccaagag cagaagcaac cuugggaggu     1140

uuuggaagcu ugggacuuga cugugaacca aggacaggcc uugacuucuc agaucuguau     1200

uaucugacca ugaacaacaa gcauugguug gugcacaagg agugguuuca ugacauccca     1260

uuaccuuggc augcuggugc agacacugga acuccacacu ggaacaacaa agaggcauug     1320

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagag     1380

ggagcuguuc acacggcccu cgcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuauucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu     1500

gugucauauu cccuguguac ugcagcguuc acauuuacca aggucccagc ugaaacauug     1560

cauggaacag uuacagugga ggugcagucu gcagggacag auggacccug caagguccca     1620

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaacuca aagaugaugu uggagcuuga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccacca cuggcauagg     1800

agugguagca ccaucggaaa ggcauuugaa gccacuguga gaggcgccaa gagaauggca     1860

guccuggggg auacagccug ggacuucgga ucagucgggg guguguucaa cucacugggu     1920

aagggcauuc accagauuuu uggagcagcc uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacgcugcua gugugguuag guuugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu ggcccugggg ggagugauga ucuuccucuc cacggcuguu     2100

ucugcugacg uggggugcuc aguggacuuc ucaaag                               2136


<210>  977
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long CDS wild type

<400>  977
agaauaauca augcuaggaa agagaggaag agacguggcg cagacaccag caucggaauc       60

auuggccucc ugcugacuac agccauggca gcagagauca cuagacgcgg gagugcauac      120

uacauguacu uggauaggag cgaugccggg aaggccauuu cguuugcuac cacaauggga      180

gugaacaagu gccacguaca gaucauggac cucgggcaca ugugugacgc caccaugagu      240

uaugagugcc cuaugcugga ugagggagug gaaccagaug augucgauug cuggugcaac      300

acgacaucaa cuuggguugu guacggaacc ugucaucaca aaaaagguga ggcacggccc      360

ucaagaagag cugucacgcu uccuucccau uccacaagaa aguuacagac acggucacaa      420

accuggcuug aaucacggga guacacgaag caccugauca aaguugagaa cuggauacuc      480

cggaauccag gguucacagu ugcugcucua gcaauggccu ggcucuuggg aaauuccacg      540

agccagcgag ugaucuaucu ggugaugauc cuguugauug ccccggcaua caguaucagg      600

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      660

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      720

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      780

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      900

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      960

aagaagauga ccgggaagag cauucaaccg gagaauuugg aguacagaau uaugcuguca     1020

gugcacggcu cccaacacag cgggaugauu gugaaugaca uaggacauga gaccgaugaa     1080

aacagagcaa aaguggaggu cacaccaaac ucaccaagag cugaagcgac ccuagggggc     1140

uucgggaguu ugggacucga uugugagcca aggaccgguu uagauuucuc cgaccuguac     1200

uauuuaacca ugaacaacaa gcauugguug gugcacaagg agugguucca cgacauucca     1260

uugccauggc acgcuggagc ggacacaggg acuccccauu ggaacaacaa agaggcauug     1320

guugaguuca aggaugccca cgccaaaaga cagacuguug ucguguuggg aagucaggag     1380

ggugccgucc acacagcccu ggcaggagcu uuggaagcgg agauggaugg agccaaagga     1440

aggcuguucu caggucauuu gaagugccgg auaaagaugg acaagcuuag guugaagggu     1500

gucucauacu cauugugcac ugccgccuuc acauucucaa agguuccggc ugaaacgcug     1560

caugggacag ucacaguuga ggugcaguau gcgggcagag auggaccuug caaggugcca     1620

gcccaaaugg caguggauau gcagacucuu accccagugg gaagguugau aacggcaaau     1680

cccaucauaa cugagagcac ugaaaacucg agaaugaugu uggagcugga cccacccuuu     1740

ggagacucuu acauugucau ugggguugga gauaagaaaa uuacccauca uuggcacaga     1800

aguggaagca cgauugggaa ggcauuugag gccacuguga gaggugcaaa aaggauggca     1860

guccugggag auaccgccug ggauuuuggu ucuguuggag gcguguucaa uucucugggc     1920

aagggaguuc aucaggucuu cggagcagcc uuuaaauccu uauuuggcgg aauguccugg     1980

uuuucacaaa uucuuauugg cacacuuuua gugugguugg gcuugaauac aaaaaaugga     2040

uccauuuccc uugcaugucu ggcgcuugga ggggcgauga ucuuucucuc cacggccguc     2100

ucggcagacg uggggugcuc aguugacuuc                                      2130


<210>  978
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short CDS wild type

<400>  978
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  979
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short CDS wild type

<400>  979
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacacgugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucacuguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg ggggguuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu ggcugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa aucuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacgcaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  980
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short CDS wild type

<400>  980
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag ucgacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaacgg uuguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugauuggaua ugaaacugac     1020

gaagauagag cgaaagucga gguuacgccu aauucaccaa gagcggaagc aaccuuggga     1080

ggcuuuggaa gcuuaggacu ugacugugaa ccaaggacag gccuugacuu uucagaucug     1140

uauuaccuga ccaugaacaa uaagcauugg uuggugcaca aagagugguu ucaugacauc     1200

ccauugccuu ggcaugcugg ggcagacacc ggaacuccac acuggaacaa caaagaggca     1260

uugguagaau ucaaggaugc ccacgccaag aggcaaaccg ucgucguucu ggggagccag     1320

gaaggagccg uucacacggc ucucgcugga gcucuagagg cugagaugga uggugcaaag     1380

ggaaggcugu ucucuggcca uuugaaaugc cgccuaaaaa uggacaagcu uagauugaag     1440

ggcgugucau auuccuugug cacugcggca uucacauuca ccaagguccc agcugaaaca     1500

cugcauggaa cagucacagu ggaggugcag uaugcaggga cagauggacc cugcaagauc     1560

ccaguccaga uggcggugga caugcagacc cugaccccag uuggaaggcu gauaaccgcc     1620

aaccccguga uuacugaaag cacugagaac ucaaagauga uguuggagcu ugacccacca     1680

uuuggggauu cuuacauugu cauaggaguu ggggacaaga aaaucaccca ccacuggcau     1740

aggaguggua gcaccaucgg aaaggcauuu gaggccacug ugagaggcgc caagagaaug     1800

gcaguccugg gggauacagc cugggacuuc ggaucagucg gggguguguu caacucacug     1860

gguaagggca uucaccagau uuuuggagca gccuucaaau cacuguuugg aggaaugucc     1920

ugguucucac agauccucau aggcacgcug cuaguguggu uagguuugaa cacaaagaau     1980

ggaucuaucu cccucacaug cuuggcccug gggggaguga ugaucuuccu cuccacggcu     2040

guuucugcu                                                             2049


<210>  981
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short CDS wild type

<400>  981
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguuggucca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  982
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short CDS wild type

<400>  982
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag ucgacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaacgg uuguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugauugucaa ugauacagga     1020

uaugaaacug acgaaaauag agcgaaaguc gagguuacgc cuaauucacc aagagcggaa     1080

gcaaccuugg gaggcuuugg aagcuuagga cuugacugug aaccaaggac aggccuugac     1140

uuuucagauc uguauuaccu gaccaugaac aauaagcauu gguuggugca caaagagugg     1200

uuucaugaca ucccauugcc uuggcaugcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagagg cauugguaga auucaaggau gcccacgcca agaggcaaac cgucgucguu     1320

cuggggagcc aggaaggagc cguucacacg gcucucgcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaagcu guucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcacugcgg cauucacauu caccaagguc     1500

ccagcugaaa cacugcaugg aacagucaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaaga ucccagucca gauggcggug gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga acucaaagau gauguuggag     1680

cuugacccac cauuugggga uucuuacauu gucauaggag uuggggacaa gaaaaucacc     1740

caccacuggc auaggagugg uagcaccauc ggaaaggcau uugaggccac ugugagaggc     1800

gccaagagaa uggcaguccu gggggauaca gccugggacu ucggaucagu cgggggugug     1860

uucaacucac uggguaaggg cauucaccag auuuuuggag cagccuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacgc ugcuagugug guuagguuug     1980

aacacaaaga auggaucuau cucccucaca ugcuuggccc uggggggagu gaugaucuuc     2040

cucuccacgg cuguuucugc u                                               2061


<210>  983
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short CDS wild type

<400>  983
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucgcaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag uugacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaacgg uuguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugauugucaa ugauacagga     1020

uaugaaacug acgaaaauag agcgaaaguc gagguuacgc cuaauucacc aagagcggaa     1080

gcaaccuugg gaggcuuugg aagcuuagga cuugacugug aaccaaggac aggucuugac     1140

uuuucagauc uguauuaccu gaccaugaac aauaagcauu gguuggugca caaagagugg     1200

uuucaugaca ucccauugcc uuggcaugcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagagg cauugguaga auucaaggau gcccacgcca agaggcaaac cgucgucguu     1320

cuggggagcc aggaaggagc cguucacacg gcucucgcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaagcu guucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcacugcgg cauucacauu caccaagguc     1500

ccagcugaaa cacugcaugg aacagucaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaaga ucccagucca gauggcggug gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga acucaaagau gauguuggag     1680

cuugacccac cauuugggga uucuuacauu gucauaggag uuggggacaa gaaaaucacc     1740

caccacuggc auaggagugg uagcaccauc ggaaaggcau uugaggccac ugugagaggc     1800

gccaagagaa uggcaguccu gggggauaca gccugggacu ucggaucagu cgggggugug     1860

uucaacucac uggguaaggg cauucaccag auuuuuggag cagccuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacgc ugcuagugug guuagguuug     1980

aacacaaaga auggaucuau cucccucaca ugcuuggccc uggggggagu gaugaucuuc     2040

cucuccacgg cuguuucugc u                                               2061


<210>  984
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short CDS wild type

<400>  984
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag uugacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaacgg uuguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugacugucaa ugauauagga     1020

uaugaaacug acgaaaauag agcgaaaguc gagguuacgc cuaauucacc aagagcggaa     1080

gcaaccuugg gaggcuuugg aagcuuagga cuugacugug aaccaaggac aggccuugac     1140

uuuucagauc uguauuaccu gaccaugaac aauaagcauu gguuggugca caaagagugg     1200

uuucaugaca ucccauugcc uuggcaugcu ggggcagaca cuggaacucc acacuggaac     1260

aacaaagagg cauugguaga auucaaggau gcccacgcca agaggcaaac cgucgucguu     1320

cuggggagcc aggaaggagc cguucacacg gcucucgcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaagcu guucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcacugcgg cauucacauu caccaagguc     1500

ccagcugaaa cacugcaugg aacagucaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaaga ucccagucca gauggcggug gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga acucaaagau gauguuggag     1680

cuugacccac cauuugggga uucuuacauu gucauaggag uuggggacaa gaaaaucacc     1740

caccacuggc auaggagugg uagcaccauc ggaaaggcau uugaggccac ugugagaggc     1800

gccaagagaa uggcaguccu gggggauaca gccugggacu ucggaucagu cgggggugug     1860

uucaacucac uggguaaggg cauucaccag auuuuuggag cagccuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacgc ugcuagugug guuagguuug     1980

aacacaaaga auggaucuau cucccucaca ugcuuggccc uggggggagu gaugaucuuc     2040

cucuccacgg cuguuucugc u                                               2061


<210>  985
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short CDS wild type

<400>  985
accagcaucg gaaucguugg ccuccuguug acuacagcca uggcagcaga gaucacuaga       60

cgugggagug cauacuacau guacuuggau aggagcgaug cagggaaggc cauuucuuuc      120

gcuaccacau ugggggugaa caaaugccau gugcagauca uggaccucgg gcacaugugu      180

gacgccacca ugagcuauga augcccuaug cuggacgagg ggguggaacc agaugacguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucauaaaaaa      300

ggugaagcac ggcgaucuag aagagccguc acgcucccau cucacuccac aaggaaauug      360

caaacgcggu cgcagacuug gcuagaauca agagaauaca caaagcaccu gaucaagguu      420

gaaaauugga uauucaggaa cccugguuuu acgcuagugg cugucgccau cgccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau aggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugacguugu ccuggaacau ggaggcugcg ucaccgugau ggcacaggac      660

aagccaacag uugacauaga gcuggucaca acaacgguua guaacauggc cgaggugaga      720

uccuauuguu acgaggcauc aauaucggac auggcuucgg acagucgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug uuugcaaaag aacauuggug      840

gacagagguu ggggaaaugg guguggacuc uuuggcaaag ggaguuuggu gacaugugcu      900

aaguucacgu gcuccaagaa gaugacuggg aagagcauuc agccggagaa ccuggaguau      960

cggauaaugc uaucagugca uggcucccag cacaguggga ugauuguuaa ugaugaaaac     1020

agagcgaagg ucgagguuac gcccaauuca ccaagagcag aagcaacccu gggaggcuuu     1080

ggaagcuuag gacuugauug ugaaccaagg acaggccuug acuuuucaga ucuguauuac     1140

cuaaccauga auaacaagca uugguuggug cacaaagagu gguuucauga caucccauug     1200

cccuggcaug cuggggcaga cacuggaacu ccacauugga acaacaagga ggcauuagug     1260

gaauucaagg acgcccacgc caagaggcaa accgucgugg uuuuggggag ccaggaagga     1320

gccguccaca cggcucuugc uggagcucua gaggcugaga uggauggugc aaagggaagg     1380

cuauucucug gccacuugaa augucgcuua aaaauggaca agcuuagauu gaagggcgug     1440

ucauauuccu ugugcaccgc ggcauucaca uucaccaagg ucccggcuga aacacuacau     1500

ggaacaguca caguggaggu gcaguaugca gggacagaug gacccugcaa ggucccagcc     1560

cagauggcgg uggacaugca gaccuugacc ccagucggaa ggcugauaac cgccaacccc     1620

gugauuacug aaagcacuga gaauucaaag augauguugg agcucgaccc accauuuggg     1680

gauucuuaca uugucauagg aguuggggau aagaaaauca cccaucacug gcauaggagu     1740

ggcagcacca uuggaaaagc auuugaagcc acugugagag gcgcuaagag aauggcaguc     1800

cugggggaca cagcuuggga cuuuggauca gucggaggug uguuuaacuc auugggcaag     1860

ggcauucauc agauuuuugg agcagcuuuc aaaucacugu uuggaggaau guccugguuc     1920

ucacagaucc ucauaggcac ucugcuggug ugguuagguc ugaacacaaa gaaugggucu     1980

aucucccuca caugcuuagc ccugggggga gugaugaucu uccucuccac ggcuguuucu     2040

gcu                                                                   2043


<210>  986
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short CDS wild type

<400>  986
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag ucgacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaacgg uuguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguac      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugauuggaua ugaaacugac     1020

gaaaauagag cgaaagucga gguuacgccu aauucaccaa gagcggaagc aaccuuggga     1080

ggcuuuggaa gcuuaggacu ugacugugaa ccaaggacag gccuugacuu uucagaucug     1140

uauuaccuga ccaugaacaa uaagcauugg uuggugcaca aagagugguu ucaugacauc     1200

ccauugccuu ggcaugcugg ggcagacacc ggaacuccac acuggaacaa caaagaggca     1260

uugguagaau ucaaggaugc ccacgccaag aggcaaaccg ucgucguucu ggggagccag     1320

gaaggagccg uucacacggc ucucgcugga gcucuagagg cugagaugga uggugcaaag     1380

ggaaagcugu ucucuggcca uuugaaaugc cgccuaaaaa uggacaagcu uagauugaag     1440

ggcgugucau auuccuugug cacugcggca uucacauuca ccaagguccc agcugaaaca     1500

cugcauggaa cagucacagu ggaggugcag uaugcaggga cagauggacc cugcaagauc     1560

ccaguccaga uggcggugga caugcagacc cugaccccag uuggaaggcu gauaaccgcc     1620

aaccccguga uuacugaaag cacugagaac ucaaagauga uguuggagcu ugacccacca     1680

uuuggggauu cuuacauugu cauaggaguu ggggacaaga aaaucaccca ccacuggcau     1740

aggaguggua gcaccaucgg aaaggcauuu gaggccacug ugagaggcgc caagagaaug     1800

gcaguccugg gggauacagc cugggacuuc ggaucagucg gggguguguu caacucacug     1860

gguaagggca uucaccagau uuuuggagca gccuucaaau cacuguuugg aggaaugucc     1920

ugguucucac agauccucau aggcacgcug cuaguguggu uagguuugaa cacaaagaau     1980

ggaucuaucu cccucacaug cuuggcccug gggggaguga ugaucuuccu cuccacggcu     2040

guuucugcu                                                             2049


<210>  987
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short CDS wild type

<400>  987
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcagggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  988
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short CDS wild type

<400>  988
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uagaaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggccuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  989
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short CDS wild type

<400>  989
acuagugucg gaauuguugg ccuccuacug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggu acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  990
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short CDS wild type

<400>  990
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacua ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aauaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacacgcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  991
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short CDS wild type

<400>  991
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagauaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacuggugga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cugcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  992
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short CDS wild type

<400>  992
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uucuagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  993
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short CDS wild type

<400>  993
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuacg cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugcccca      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcuggucaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg caguaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  994
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short CDS wild type

<400>  994
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacugaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cuccuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ucgucucugc u                                               2061


<210>  995
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short CDS wild type

<400>  995
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucagcuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggc cgcaaaccug guuggaauca agagaauaca caaagcauuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacaagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  996
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short CDS wild type

<400>  996
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaaguu caacgagcaa aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auugggacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  997
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short CDS wild type

<400>  997
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgcgcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacugaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cuccuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  998
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short CDS wild type

<400>  998
acuagugucg gaaucguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggggguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagcuuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggacuc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  999
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short CDS wild type

<400>  999
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac caggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcauuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacaagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagcc ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucug acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1000
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short CDS wild type

<400>  1000
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggau agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagccgug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaauc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugauaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagccca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1001
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short CDS wild type

<400>  1001
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga gugcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uacucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uauuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cucagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggaa ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1002
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short CDS wild type

<400>  1002
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuuca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcaac aauaucagac auagcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugccgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1003
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short CDS wild type

<400>  1003
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac caggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucug acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc gagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggaguuac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1004
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short CDS wild type

<400>  1004
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcgcuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauauagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccaccuugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcauugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggugcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc aucggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1005
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short CDS wild type

<400>  1005
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gaugguguaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaaaau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu aggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uuccuuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1006
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short CDS wild type

<400>  1006
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuacau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgccuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaagcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg ccaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccucaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1007
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short CDS wild type

<400>  1007
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaaagaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagccgug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccugguuuc gcuuuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug uuugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugu ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc auaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1008
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short CDS wild type

<400>  1008
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuacau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagcuuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggaa gcacaugcua aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg ccaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccucaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1009
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short CDS wild type

<400>  1009
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugc      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaauacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccgcccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccugu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaacucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1010
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short CDS wild type

<400>  1010
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucaccaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuggug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcauu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg ggcagaugga     1560

cccugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg ccaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1011
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short CDS wild type

<400>  1011
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagccgug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccugguuuc gcuuuagcag cagcugccau cgcguggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugcccug      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugu ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagccca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1012
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short CDS wild type

<400>  1012
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcaga ggucacuaga       60

cgugggagug cauacuacau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac augguuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uucucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg ccaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccucaug ugcuuggccu uagggggagu gcugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1013
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short CDS wild type

<400>  1013
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuacau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau agcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggaaucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaguaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aauccuagga cuggauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg ccaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccucaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1014
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short CDS wild type

<400>  1014
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga agucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacacgugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccau uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaaaguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg ggggguuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu ggcugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcaugg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacgcaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1015
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short CDS wild type

<400>  1015
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuacau guacuuggac agaaacgaug cuggggaggc cauaucuuuc      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac agagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaaguuaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggaggcugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acccaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuggauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg ccaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagauaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccucaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1016
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short CDS wild type

<400>  1016
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug ccggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caagcgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauauagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug uugacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagcuuggu gacaugcgcu      900

aaguuugcau gcuccaagaa gaugaccggg aagagcaucc agccagagaa uuuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaacag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaagagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggugcu     1860

cuuaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1017
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short CDS wild type

<400>  1017
acuagugucg gaauuguugg ccuccugcug accacagcca uggcagcgga agucacuaga       60

cgugggagug cauacuauau guacuuggac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacac uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguagaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ccacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuggcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu cuccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca gaggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccggu aaucacugaa agcacagaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aauacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1018
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short CDS wild type

<400>  1018
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacac uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguagaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagauccag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagu aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaac aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacacu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa ggcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcguu     1860

cuuaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aauacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucuccgc u                                               2061


<210>  1019
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short CDS wild type

<400>  1019
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacac uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguagaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaguca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagau acccaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggaaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucagucca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguuggucca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggacaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

cccugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg caguaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggccug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1020
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short CDS wild type

<400>  1020
acuagugucg gaauuguugg ccuccugcug accacagcca uggcagugga ggucacuaga       60

cgugggaaug cauacuauau guacuuggac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacaa uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguagaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ccacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaca caaagcaccu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug uuaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcuu gcucuaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugauacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcaugcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcagac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu cacuaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccugu aaucacugaa agcacugaga acuccaagau gaugcuggaa     1680

cuggauccac cauuugggga cucuuacauu gucauaggag ucggggaaaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu ugggggugcu     1860

cucaacucac ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcuggugug guugggucug     1980

aauacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1021
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short CDS wild type

<400>  1021
acuagugucg gaauuguugg ccuccugcug accacagcca uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacac uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguagaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ccacaaaaaa      300

ggugaagcgc ggagaucuag aagagcugug acgcuccccu cccacuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuggcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauauagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugaugucgu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc agagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcc      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cgaauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gagguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggau gcacaugcca aaaggcaaac ugucgugguc     1320

cuagggaguc aagagggagc aguucacacg gcucuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagacuga agggcguguc auacucccug uguaccgcag cguucacauu cacuaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccugu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg uauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aauacaaaga auggaucuau uucccuugug ugcuuggccu uagggggagu guugaucuuc     2040

uuguccacag ccgucucugc u                                               2061


<210>  1022
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short CDS wild type

<400>  1022
acuagugucg gaauuguugg ccuccugcug accacagcca uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacac uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguagaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ccacaaaaaa      300

ggugaagcgc ggagaucuag aagagcugug acgcuccccu cccacuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuggcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauauagca ucaggugcau aggagucaac aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugaugucgu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc agagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcc      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cgaauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gagguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggau gcacaugcca aaaggcaaac ugucgugguc     1320

cuagggaguc aagagggagc aguucacacg gcucuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagacuga agggcguguc auacucccug uguaccgcag cguucacauu cacuaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccugu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg uauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aauacaaaga auggaucuau uucccuugug ugcuuggccu uagggggagu guugaucuuc     2040

uuguccacag ccgucucugc u                                               2061


<210>  1023
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short CDS wild type

<400>  1023
acuagugucg gaauuguugg ccuccugcug accacagcca uggcagugga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacac uggggaugaa caaguguuac auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug uuggaugagg ggguagaacc agaugacguc      240

gauuguuggu gcaacacgac aucaacuugg guuguguacg gaaccugcca ccacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaua caaagcaccu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgccuggcuu      480

uugggaaguu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug uuaccguaau ggcacaggac      660

aaaccggcug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuauugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcagucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagcugaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcaugcu ggggcagaca ccggaacucc acauuggaac     1260

aacaaagaag cauugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

cccugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

cugauaaccg cuaacccugu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caucacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggauu uuggaucagu uggaggugcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcuggugug guugggucug     1980

aauacaaaga auggaucuau uucccuuacg ugcuuggccu uagggggagu guugaucuuu     2040

uuauccacag ccgucucugc u                                               2061


<210>  1024
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short CDS wild type

<400>  1024
acuagcgucg gaauuguugg ccuccuccug accacagcca uggcaguaga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacac uggggaugaa uaaguguuac auacaaauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug uuggaugagg ggguagaacc agaugacguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguaug gaaccugcca ccacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaca caaagcaccu gauuagaguu      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugucau cgcuuggcuu      480

uugggaaguu caacgagcca aaaagucaua uaucugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug uuaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggau auggcuucgg acagccgcug cccaacacaa      780

ggugaggccu accuugacaa gcagucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg gagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuaccu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcaugcu ggggcagaca cuggaacucc acauuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcaa aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggagcca agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug ugcacugcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gauaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccugu aaucacugaa agcaccgaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caucacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu ugggggugcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuc     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcuggugug guugggucug     1980

aauacaaaga auggaucuau uucccuuacg ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccguuucugc u                                               2061


<210>  1025
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short CDS wild type

<400>  1025
accagugucg gaauuguugg ccuccugcug accacagcca uggcagugga ggucaccaga       60

cgugggagug cauacuauau guacuuagac agaagcgaug cuggggaggc cauaucuuuu      120

ccaaccacac ugggggugaa uaaguguuac auacagauca uggaucuugg acacaugugu      180

gaugccacaa ugagcuauga augcccuaug uuggaugagg ggguagaacc agaugacguc      240

gauugcuggu gcaacacgac aucgacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaggcac ggagaucuag aagagcugug acgcuccccu cucauuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaca caaagcacuu gaucagaguc      420

gaaaauugga uauucaggaa cccuggcuuu gcguuggcag cagcugccau ugcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacuguu gauugccccg      540

gcauacagua ucaggugcau aggagucagc aauagggauu uuguggaagg uaugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggagguugug uuaccguaau ggcacaggac      660

aagccaacug uugauauaga guuggucaca acaacgguua gcaacauggc ggagguaaga      720

uccuacugcu acgaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcagucagac acucaauaug uuugcaaaag aacguuagug      840

gacagagguu ggggaaaugg auguggacuc uuuggcaaag ggagccuggu gacaugcgcc      900

aaguuugcau gcuccaagaa aaugacuggg aagagcaucc agccagagaa ccuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugauuguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gagguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcauu gguuggugca caaggagugg     1200

uuccaugaca uuccacuacc uuggcaugcu ggggcagaca ccggaacucc acauuggaac     1260

aacaaagaag cauugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc cguucacacg gcucuugcug gagcccugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgcuugaa aauggacaaa     1440

cuuagauuga agggcguguc auacuccuua uguaccgcgg cguucacauu caccaagauc     1500

ccggcugaaa cgcugcaugg gacagucaca guggagguac aguaugcagg gacagaugga     1560

cccugcaagg uuccagcuca gauggcggug gauaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccugu gaucacugaa agcacugaga auucaaagau gauguuggaa     1680

cuugacccac cauuugggga uucuuacauu gucauaggag uuggggauaa gaagaucacc     1740

caccacuggu acaggagugg cagcaccauc ggaaaagcau uugaagccac ugugagaggc     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu cggaggugcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auaggaacgu ugcuggugug guugggucug     1980

aacacaaaga auggaucuau uucccuuacg ugcuuggccu uagggggagu guugaucuuc     2040

cuaucuacag ccgucucugc u                                               2061


<210>  1026
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short CDS wild type

<400>  1026
accagugucg gaauuguugg ccuccugcug accacagcca uggcagugga ggucaccaga       60

cgugggagug cauacuauau guacuuagac agaagcgaug cugggaaggc cauaucuuuu      120

ccaaccacac ugggggugaa uaaguguuac auacagauca uggaucuugg acacaugugu      180

gaugccacaa ugagcuauga augcccuaug uuggaugagg ggguagaacc agaugacguc      240

gauugcuggu gcaacacgac aucgacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaggcac ggagaucuag aagagcugug acgcuccccu cucauuccac uaggaagcug      360

caaacgcggu cgcagaccug guuggaauca agagaauaca caaagcacuu gaucagaguc      420

gaaaauugga uauucaggaa cccuggcuuu gcguuggcag cagcugccau ugcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacuguu gauugccccg      540

gcauacagua ucaggugcau aggagucagc aauagggauu uuguggaagg uaugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggagguugug uuaccguaau ggcacaggac      660

aagccaacug uugauauaga guuggucaca acaacgguua gcaacauggc ggagguaaga      720

uccuacugcu acgaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcagucagac acucaauaug uuugcaaaag aacguuagug      840

gacagagguu ggggaaaugg auguggacuc uuuggcaaag ggagccuggu gacaugcgcc      900

aaguuugcau gcuccaagaa aaugacuggg aagagcaucc agccagagaa ccuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugauuguuaa ugacauagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gagguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcauu gguuggugca caaggagugg     1200

uuccaugaca uuccacuacc uuggcaugcu ggggcagaca ccggaacucc acauuggaac     1260

aacaaagaag cauugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc cguucacacg gcucuugcug gagcccugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgcuugaa aauggacaaa     1440

cuuagauuga agggcguguc auacuccuua uguaccgcgg cguucacauu caccaagauc     1500

ccggcugaaa cgcugcaugg gacagucaca guggagguac aguaugcagg gacagaugga     1560

cccugcaagg uuccagcuca gauggcggug gauaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccugu gaucacugaa agcacugaga auucaaagau gauguuggaa     1680

cuugacccac cauuugggga uucuuacauu gucauaggag uuggggauaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauc ggaaaagcau uugaagccac ugugagaggc     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu cggaggugcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauccuc auaggaacgu ugcuggugug guugggucug     1980

aacacaaaga auggaucuau uucccuuacg ugcuuggccu uagggggagu guugaucuuc     2040

cuaucuacag ccgucucugc u                                               2061


<210>  1027
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short CDS wild type

<400>  1027
accagcaucg gaaucguugg ccuccuguug acuacagcca uggcagcaga gaucacuaga       60

cgugggagug cauacuacau guacuuggau aggagcgaug cagggaaggc cauuucuuuc      120

gcuaccacau ugggggugaa caaaugccau gugcagauca uggaccucgg gcacaugugu      180

gacgccacca ugagcuauga augcccuaug cuggacgagg ggguggaacc agaugacguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucauaaaaaa      300

ggugaagcac ggcgaucuag aagagccguc acgcucccau cucacuccac aaggaaauug      360

caaacgcggu cgcagacuug gcuagaauca agagaauaca caaagcaccu gaucaagguu      420

gaaaauugga uauucaggaa cccugguuuu acgcuagugg cugucgccau cgccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau aggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugacguugu ccuggaacau ggaggcugcg ucaccgugau ggcacaggac      660

aagccaacag uugacauaga gcuggucaca acaacgguua guaacauggc cgaggugaga      720

uccuauuguu acgaggcauc aauaucggac auggcuucgg acagucgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug uuugcaaaag aacauuggug      840

gacagagguu ggggaaaugg guguggacuc uuuggcaaag ggaguuuggu gacaugugcu      900

aaguucacgu gcuccaagaa gaugacuggg aagagcauuc agccggagaa ccuggaguau      960

cggauaaugc uaucagugca uggcucccag cacaguggga ugauuguuaa ugacacagga     1020

caugaaacug augaaaacag agcgaagguc gagguuacgc ccaauucacc aagagcagaa     1080

gcaacccugg gaggcuuugg aagcuuagga cuugauugug aaccaaggac aggccuugac     1140

uuuucagauc uguauuaccu aaccaugaau aacaagcauu gguuggugca caaagagugg     1200

uuucaugaca ucccauugcc cuggcaugcu ggggcagaca cuggaacucc acauuggaac     1260

aacaaggagg cauuagugga auucaaggac gcccacgcca agaggcaaac cgucgugguu     1320

uuggggagcc aggaaggagc cguccacacg gcucuugcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaggcu auucucuggc cacuugaaau gucgcuuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcaccgcgg cauucacauu caccaagguc     1500

ccggcugaaa cacuacaugg aacagucaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaagg ucccagccca gauggcggug gacaugcaga ccuugacccc agucggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag uuggggauaa gaaaaucacc     1740

caucacuggc auaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggc     1800

gcuaagagaa uggcaguccu gggggacaca gcuugggacu uuggaucagu cggaggugug     1860

uuuaacucau ugggcaaggg cauucaucag auuuuuggag cagcuuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacuc ugcuggugug guuaggucug     1980

aacacaaaga augggucuau cucccucaca ugcuuagccc uggggggagu gaugaucuuc     2040

cucuccacgg cuguuucugc u                                               2061


<210>  1028
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short CDS wild type

<400>  1028
accagcaucg gaaucauugg ucuccugcug acuacagcca uggcagcgga gauuaccaga       60

cguggaagug cauacuacau guacuuggac aggagcgaug cugggaaggc caucuccuuu      120

gcuaccaacu ugggaguuaa caaaugccau guacagauca uggaucucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug uuggacgagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaagcac ggcgaucuag aagagcugug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcaccu gaucaagguu      420

gaaaacugga uauuuaggaa ccccggguuu gcgcucgcag cuguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguua ugauacugcu gauugccccg      540

gcauacagca ucaggugcau uggagucagc aauagagacu ucguggaagg caugucaggu      600

gggaccuggg uugaugucgu cuuggaacau ggagguugcg ucaccguuau ggcacaggac      660

aagccaacag uugacauaga guuggucacg acaacgguua guaacauggc cgagguaaga      720

uccuacuguu augaggcauc aauaucagac auggcuucgg acagucgcug cccaacacaa      780

ggugaagccu accuugauaa gcaaucagac acccaauaug ugugcaaaag aacauuggug      840

gacagagguu ggggaaaugg guguggacuu uuuggcaaag ggagccuggu gacaugugcc      900

aaguucacgu guuccaagaa gaugacuggg aagagcauuc agccggagaa ucuggaguau      960

cgaauaaugc uaucagugca uggcucccag cacagcggga ugauuguuaa ugacauagga     1020

caugaaacug acgaaaacag agcgaaaguc gaggucacgc ccaauucacc aagagcagaa     1080

gcaaccuugg gaggcuuugg aagcuuagga cuugauugug aaccaaggac aggccuggac     1140

uuuucagauu uguacuacuu gaccaugaac aacaagcacu gguuggugca caaagagugg     1200

uuucaugaca ucccauugcc uuggcaugcu ggggcagaua ccggaacucc acacuggaac     1260

aacaaggagg cauugguaga guucaaagau gcccacgcca agaggcaaac cgucguuguu     1320

cuagggagcc aggaaggagc cguucacacg gcucuugcug gagcucugga ggcugagaug     1380

gauggugcaa aaggaaggcu guucuccggc cauuugaaau gccgcuuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcaccgcag cguucacauu uaccaagguc     1500

ccagcugaaa cauugcaugg aacaguuaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaagg ucccagccca gauggcggug gacaugcaga cccugacccc agucgggagg     1620

cugauaaccg ccaacccugu gauuacugaa agcacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag uuggggacaa gaaaaucacc     1740

caucacuggc auaggagugg uagcaccauc ggaaaagcau uugaggccac ugugagaggu     1800

gccaagagaa uggcaguucu gggggauaca gccugggacu ucggaucagu cgggggcgug     1860

uucaacucau ugggcaaggg uguucaccag auuuuuggag cagcuuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacgc ugcuggugug guuggguuug     1980

aacacaaaga auggauccau cucccucaca ugcuuggccc uggggggagu gaugaucuuc     2040

cucuccacgg cugucucugc u                                               2061


<210>  1029
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short CDS wild type

<400>  1029
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaggcac ggcgaucuag aagagccgug acgcucccuu cucacucuac aaggaaguug      360

caaacgcggu cgcagaccug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcuagugg ccguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagua ucaggugcau uggagucagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggaggcugcg uuaccgugau ggcacaggac      660

aagccaacag uugacauaga guuggucacg acgacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc gauaucggac auggcuucgg acagucguug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacauuagug      840

gacagagguu ggggaaaugg uuguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguucacgu guucuaagaa gaugaccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc uaucagugca uggcucccag cauagcggga ugauugucaa ugauauagga     1020

caugaaacug acgaaaacag agcgaaaguc gagguuacgc cuaauucacc aagagcggaa     1080

gcaaccuugg gaggcuuugg aagcuuagga cuugacugug aaccaaggac aggccuugac     1140

uuuucagauc uguauuaccu gaccaugaac aauaagcauu gguuggugca caaagagugg     1200

uuucaugaca ucccauugcc uuggcaugcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagagg cauugguaga auucaaggau gcccacgcca agaggcaaac cgucgucguu     1320

cuggggagcc aggaaggagc cguucacacg gcucucgcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaggcu guucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcacugcgg cauucacauu caccaagguc     1500

ccagcugaaa cacugcaugg aacagucaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaagg ucccagccca gauggcggug gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga acucaaagau gauguuggag     1680

cuugacccac cauuugggga uucuuacauu gucauaggag uuggggacaa gaaaaucacc     1740

caccacuggc auaggagugg uagcaccauc ggaaaggcau uugaagccac ugugagaggc     1800

gccaagagaa uggcaguccu gggggauaca gccugggacu ucggaucagu cgggggugug     1860

uucaacucac uggguaaggg cauucaccag auuuuuggag cagccuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacgc ugcuagugug guuagguuug     1980

aacacaaaga auggaucuau cucccucaca ugcuuggccc uggggggagu gaugaucuuc     2040

cucuccacgg cuguuucugc u                                               2061


<210>  1030
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short CDS wild type

<400>  1030
accagcaucg gaaucauugg ucuccugcug acuacagcca uggcagcaga gauuaccaga       60

cguggaagug cauacuacau guacuuggac aggagcgaug cugggaaggc caucuccuuu      120

gcuaccaauu ugggaguuaa caaaugccau guacagauca uggaucucgg gcacaugugu      180

gacgccacca ugaguuauga guguccuaug uuggacgagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugaagcac ggcgaucuag aagagcugug acgcucccuu cucacucuac aaggaagcug      360

caaacgcggu cgcagacuug guuagaauca agagaauaca cgaagcacuu gaucaagguu      420

gaaaacugga uauucaggaa ccccggguuu gcgcucgcag cuguugccau ugccuggcuu      480

uugggaagcu cgacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau uggagucagc aauagagacu ucguggaagg caugucaggu      600

gggaccuggg uugaugucgu cuuggaacau ggagguugcg ucaccguaau ggcacaggac      660

aagccaacag uugacauaga guuggucacg acaacgguca guaacauggc cgagguaaga      720

uccuacuguu augaggcauc aauaucggac auggcuucgg acagucgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug uaugcaaaag aacauuggug      840

gacagagguu ggggaaaugg guguggacuu uuuggcaaag ggagccuggu gacaugugcc      900

aaguucacgu guuccaagaa gaugaccggg aagagcauuc agccggagaa ucuggaguau      960

cgaauaaugc uaucagugca uggcucccag cacagcggga ugauuguuaa ugacgaaaau     1020

agggcgaaag ucgaggucac gcccaauuca ccaagagcag aagcaaccuu gggaggcuuu     1080

ggaagcuuag gacuugauug ugaaccaagg acaggccugg acuuuucaga uuuguacuac     1140

uugaccauga acaauaagca cugguuagug cacaaagagu gguuucauga caucccauug     1200

cccuggcaug cuggggcaga caccggaacu ccacauugga acaacaagga ggcauuggua     1260

gaguucaaag augcccacgc uaagaggcaa accgucguug uucuagggag ccaggaagga     1320

gccguucaca cggcucuugc uggagcucug gaggcugaga uggauggugc aaaaggaagg     1380

uuguucuccg gccauuugaa augccgcuua aaaauggaca agcuuagauu gaagggcgug     1440

ucauauuccu ugugcaccgc agcguucaca uucaccaagg ucccagcuga aacauugcau     1500

ggaacaguua caguggaggu gcaguaugca gggacagaug gacccugcaa ggucccagcu     1560

cagauggcgg uggacaugca gacccugacc ccagucggaa ggcugauaac cgccaacccc     1620

gugauuacug aaagcacuga gaauucaaag augauguugg agcucgaccc accauuuggg     1680

gauucuuaca uugucauagg aguuggggac aagaaaauua cccaccacug gcauaggagu     1740

gguagcacca ucggaaaagc auuugaggcc acugugagag gugccaagag aauggcaguu     1800

cugggggaca cagccuggga cuuuggauca gucgggggcg uguuuaacuc auugggcaag     1860

ggcauccacc agauuuuugg agcagccuuc aaaucacugu uuggaggaau guccugguuc     1920

ucacagaucc ucauaggcac gcugcuggug ugguuggguu ugaacacaaa gaauggaucc     1980

aucucccuca caugcuuggc ccugggggga gugaugaucu uccucuccac ggcugucucu     2040

gcu                                                                   2043


<210>  1031
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short CDS wild type

<400>  1031
accagcaucg gaaucgucgg ccuccugcug accacagcca uggcagccga gaucacuaga       60

cgugggagug cauacuacau guacuuggac aggagcgaug cugguaaggc cauuucuuuu      120

gccaccacau ugggggugaa caaaugccau guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugccccaug cuagacgagg gaguggagcc agaugacguc      240

gauugcuggu gcaacacgac aucgacuugg guuguguacg gaaccuguca ucauaaaaaa      300

ggugaagcac gacgauccag aagagccgug acgcuuccuu cucacuccac gaggaagcug      360

caaacgcgau cgcagacuug gcuagaguca agagaauaca caaagcaccu gaucaagguu      420

gaaaauugga uauucaggaa ccccggguuu gcgcuagugg cuguagcuau agccuggcuc      480

cugggaagcu cgacgagcca aaaaguuaua uacuugguca ugauauuguu gauugccccg      540

gcauacagca uuaggugcau aggaguuagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggggguugcg ucaccgugau ggcacaggac      660

aagccaacag uugauaucga guuggucacg acaacgguua gcaacauggc cgagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccguug uccaacacaa      780

ggugaagccu accuugacaa gcagucagac acucaauaug ucugcaagag aacauuggug      840

gauagagguu ggggaaaugg guguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu gcuccaagaa aaugacaggc aagagcaucc agccggagaa cuuggaguac      960

cggauaaugc uaucagugca uggaucccag cacaguggga ugauugugaa ugacacagga     1020

caugaaacug acgaaaacag agcaaaaguc gaggucacac ccaauucacc aagagcagaa     1080

gcaaccuugg gagguuuugg aagcuuggga cuugacugug aaccaaggac aggccuugac     1140

uucucagauc uguauuacuu gaccaugaac aauaagcauu gguuggugca caaggagugg     1200

uuucaugaca ucccauuacc uuggcaugcu ggugcagaca cuggaacucc acacuggaac     1260

aacaaagagg cauuggugga guucaaggac gcccacgcca agaggcaaac uguugugguu     1320

cuggggagcc aagagggagc uguucauacg gcccucgcug gagcuuugga ggcugagaug     1380

gauggugcaa agggaaggcu auucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagguuga aggguguguc auauucccug uguaccgcag cguucacauu uaccaagguc     1500

ccagcugaaa cauugcaugg aacagucaca guggaggugc aguaugcagg gacagacgga     1560

cccugcaaag ucccagccca gauggcggug gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaauccugu gaucacugaa aguacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag ucggggacaa gaaaaucacc     1740

caucacuggc aucggagugg uagcaccauc ggaaaggcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggggacaca gccugggacu uuggaucagu ugggggugug     1860

uuuaauucau uggguaaggg uauucaccag aucuuuggag cagcuuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacac uguuggugug guuagguuug     1980

aacacaaaga auggaucuau cucccucaca ugcuuagccc uggggggagu gaugaucuuc     2040

cuuuccacgg cuguuucugc u                                               2061


<210>  1032
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short CDS wild type

<400>  1032
accagcaucg gaaucgucgg ccuccugcug accacagcca uggcagccga gaucaccaga       60

cgugggagug cauacuacau guacuuggac aggagcgaug cugguaaggc cauuucuuuu      120

gcuaccacau ugggggugaa caaaugccau guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugccccaug cuggacgagg gaguggagcc agaugacguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca acauaaaaaa      300

ggugaagcac gacgauccag aagagccgug acgcuuccuu cucacuccac aaggaagcug      360

caaacgcgau cacagacuug gcuagaauca agagaauaca caaagcaccu gaucaagguu      420

gagaauugga uauucaggaa ccccggguuu gcgcuagugg cuguagcuau ugccuggcuc      480

cugggaagcu cgacgagcca aaaagucaua uacuugguaa ugauauuguu gauugccccg      540

gcauacagca ucaggugcau aggaguuagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggggguugcg ucaccgugau ggcacaggac      660

aagccaacag uugacaucga guuggucacg acaacgguua gcaacauggc cgagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug uccaacacaa      780

ggugaagccu accuugacaa gcagucagac acucaauaug ucugcaagag aacauuggug      840

gauagagguu ggggaaaugg guguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu gcuccaagaa aaugacaggc aagagcaucc agccggagaa cuuggaguac      960

cggauaaugc uaucagugca uggaucccag cacaguggga ugauugugaa ugacauagga     1020

caugaaacug acgaaaacag agcaaaaguc gaggucacac ccaauucacc aagagcagaa     1080

gcaaccuugg gagguuuugg aagcuuggga cuugacugug aaccaaggac aggccuugac     1140

uucucagauc uguauuaccu gaccaugaac aauaagcauu gguuggugca caaggagugg     1200

uuucaugaca ucccauuacc uuggcaugcu ggugcagaca cuggaacucc acacuggaac     1260

aacaaagagg cauuggugga guuuaaggac gcccacgcca agaggcaaac uguugugguu     1320

cuggggagcc aagagggagc uguucacacg gcccucgcug gagcuuugga ggcugagaug     1380

gauggugcaa agggaaggcu auucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagguuga aggguguguc auauucccug uguaccgcag uguguaccgc agcgaagguc     1500

ccagcugaaa cauugcaugg aacagucaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaagg ucccagccca gauggcggua gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaacccugu gaucacugaa aguacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag ucggggacaa gaaaaucacc     1740

caucacuggc aucggagugg uagcaccauc ggaaaggcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggggacaca gccugggacu uuggaucagu ugggggugug     1860

uuuaauucau uggguaaggg uauucaccag aucuuuggag cagcuuuuaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacac uguuggugug guuaggucug     1980

aacacaaaga auggaucuau cucccucaca ugcuuagccc uggggggagu gaugaucuuc     2040

cuuuccacgg cuguuucugc u                                               2061


<210>  1033
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short CDS wild type

<400>  1033
accagcaucg gaaucgucgg ccuccugcug accacagcca uggcagccga gaucacuaga       60

cgugggagug cauacuacau guacuuggac aggagcgaug cugguaaggc cauuucuuuu      120

gccaccacau ugggggugaa caaaugccau guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugccccaug cuagacgagg gaguggagcc agaugacguc      240

gauugcuggu gcaacacgac aucgacuugg guuguguacg gaaccuguca ucauaaaaaa      300

ggugaagcac gacgauccag aagagccgug acgcuuccuu cucacuccac gaggaagcug      360

caaacgcgau cgcagacuug gcuagaguca agagaauaca caaagcaccu gaucaagguu      420

gaaaauugga uauucaggaa ccccggguuu gcgcuagugg cuguagcuau agccuggcuc      480

cugggaagcu cgacgagcca aaaaguuaua uacuugguca ugauauuguu gauugccccg      540

gcauacagca uuaggugcau aggaguuagc aauagagacu ucguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggggguugcg ucaccgugau ggcacaggac      660

aagccaacag uugauaucga guuggucacg acaacgguua gcaacauggc cgagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccguug uccaacacaa      780

ggugaagccu accuugacaa gcagucagac acucaauaug ucugcaagag aacauuggug      840

gauagagguu ggggaaaugg guguggacuu uuuggcaaag ggagcuuggu gacaugugcc      900

aaguuuacgu gcuccaagaa aaugacaggc aagagcaucc agccggagaa cuuggaguac      960

cggauaaugc uaucagugca uggaucccag cacaguggga ugauugugaa ugacgaaaac     1020

agagcaaaag ucgaggucac acccaauuca ccaagagcag aagcaaccuu gggagguuuu     1080

ggaagcuugg gacuugacug ugaaccaagg acaggccuug acuucucaga ucuguauuac     1140

cugaccauga acaauaagca uugguuggug cacaaggagu gguuucauga caucccauua     1200

ccuuggcaug cuggugcaga cacuggaacu ccacacugga acaacaaaga ggcauuggug     1260

gaguucaagg acgcccacgc caagaggcaa acuguugugg uucuggggag ccaagaggga     1320

gcuguucaua cggcccucgc uggagcuuug gaggcugaga uggauggugc aaagggaagg     1380

cuauucucug gccauuugaa augccgccua aaaauggaca agcuuagguu gaagggugug     1440

ucauauuccc uguguaccgc agcguucaca uuuaccaagg ucccagcuga aacauugcau     1500

ggaacaguca caguggaggu gcaguaugca gggacagacg gacccugcaa agucccagcc     1560

cagauggcgg uggacaugca gacccugacc ccaguuggaa ggcugauaac cgccaauccu     1620

gugaucacug aaaguacuga gaauucaaag augauguugg agcucgaccc accauuuggg     1680

gauucuuaca uugucauagg agucggggac aagaaaauca cccaucacug gcaucggagu     1740

gguagcacca ucggaaaggc auuugaagcc acugugagag gugccaagag aauggcaguc     1800

uugggggaca cagccuggga cuuuggauca guugggggug uguuuaauuc auuggguaag     1860

gguauucacc agaucuuugg agcagcuuuc aaaucacugu uuggaggaau guccugguuc     1920

ucacagaucc ucauaggcac acuguuggug ugguuagguu ugaacacaaa gaauggaucu     1980

aucucccuca caugcuuagc ccugggggga gugaugaucu uccuuuccac ggcuguuucu     2040

gcu                                                                   2043


<210>  1034
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short CDS wild type

<400>  1034
accagcaucg gaaucauugg ccuccugcug acuacagcca uggcagcaga gaucacuaga       60

cgcgggagug cauacuacau guacuuggau aggagcgaug ccgggaaggc cauuucguuu      120

gcuaccacau ugggagugaa caagugccac guacagauca uggaccucgg gcacaugugu      180

gacgccacca ugaguuauga gugcccuaug cuggaugagg gaguggaacc agaugauguc      240

gauugcuggu gcaacacgac aucaacuugg guuguguacg gaaccuguca ucacaaaaaa      300

ggugagacac ggcgaucuag aagaucugug ucgcuccguu aucacuauac aaggaaguug      360

caaacgcggu cgcagacaug guuagaauca agagaauaca agaagcacuu gaucaugguc      420

gaaaacugga uauucaggaa ccccggguuu gccauagugu ccguugccau uaccuggcug      480

augggaagcu ugacgagcca aaaagucaua uacuugguca ugauaguguu gauugucccg      540

gcauacagua ucagcugcau uggagucagc aauagagacu uaguggaggg caugucaggu      600

gggaccuggg uugauguugu cuuggaacau ggagggugcg uuaccgagau ggcacaggac      660

aagccaacag uugacauaga guuggucacg augacgguua guaacauggc cgagguaaga      720

uccuauugcu acgaggcauc guuauccgac auggcuucgg ccagucguug cccaacacaa      780

ggcgaacccu cccucgacaa gcaaucagac acucaaucug uaugcaaaag aacauuagga      840

gacagagguu ggggaaaugg uugugggauu uuuggcaaag ggagcuuggu gacauguucc      900

aaguucacgu guuguaagaa gaugcccggg aagagcauuc aaccggaaaa ucuggaguau      960

cggauaaugc ucccagugca uggcucccag cauagcggga ugauugugaa ugacauagga     1020

caugaaacug acgaaaacag agcgaaaguc gaggucacac ccaauucacc aagagcagaa     1080

gcaaccuugg gagguuuugg aagcuuggga cuugacugug aaccaaggac aggccuugac     1140

uucucagauc uguauuaucu gaccaugaac aacaagcauu gguuggugca caaggagugg     1200

uuucaugaca ucccauuacc uuggcaugcu ggugcagaca cuggaacucc acacuggaac     1260

aacaaagagg cauuggugga guucaaggac gcccacgcca agaggcaaac uguugugguu     1320

cuggggagcc aagagggagc uguucacacg gcccucgcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu auucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagguuga aggguguguc auauucccug uguacugcag cguucacauu uaccaagguc     1500

ccagcugaaa cauugcaugg aacaguuaca guggaggugc agucugcagg gacagaugga     1560

cccugcaagg ucccagccca gauggcggug gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga acucaaagau gauguuggag     1680

cuugacccac cauuugggga uucuuacauu gucauaggag uuggggacaa gaaaaucacc     1740

caccacuggc auaggagugg uagcaccauc ggaaaggcau uugaagccac ugugagaggc     1800

gccaagagaa uggcaguccu gggggauaca gccugggacu ucggaucagu cgggggugug     1860

uucaacucac uggguaaggg cauucaccag auuuuuggag cagccuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacgc ugcuagugug guuagguuug     1980

aacacaaaga auggaucuau cucccucaca ugcuuggccc uggggggagu gaugaucuuc     2040

cucuccacgg cuguuucugc u                                               2061


<210>  1035
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short CDS wild type

<400>  1035
agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu       60

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      120

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      180

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      240

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      300

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      360

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      420

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      480

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      540

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      600

acuuggguug auguugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      660

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      720

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      780

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      840

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gccuggugac augcgcuaag      900

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg      960

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1020

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1080

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1140

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1200

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1260

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1320

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1380

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1440

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1500

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1560

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1620

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1680

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1740

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1800

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1860

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1920

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     1980

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2040

uccacagccg ucucugcu                                                   2058


<210>  1036
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short CDS wild type

<400>  1036
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaacuug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acuccaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaggagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1037
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short CDS wild type

<400>  1037
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaacugga uguucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg ucccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1038
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short CDS wild type

<400>  1038
accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacaugac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucggac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagccuggu gaccugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguccacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1620

uugauaaccg cuaacccugu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg uagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     1980

aacacaaaga acggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucugc u                                               2061


<210>  1039
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short CDS wild type

<400>  1039
acuagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga       60

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      120

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      180

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      240

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      300

ggugaagcac ggagaucuag aagagcugug acgcucccuu cccauuccac uaggaagcug      360

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      420

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      480

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      540

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      600

gggacuuggg uugauguugu cuuggaacau ggagguugug ucaccgcaau ggcacaggac      660

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      720

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      780

ggugaagccu accuugacaa gcaaucagac acucaauaug uuugcaaaag aacguuagug      840

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      900

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac      960

cggauaaugc ugucaguuca uggcucccag cacaguggga ugcucguuaa ugacacagga     1020

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1080

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1140

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguuggcuca caaggagugg     1200

uuccacgaca uuccauuacc uuggcacgcu ggggcagcca ccggaacucc acacuggaac     1260

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1320

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1380

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1440

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1500

ccggcugaaa caguggacgg gacagucaca guggagggac aguacggagg gacagaugga     1560

ccuugcaagg uuccagcuca gauggcggug gacaugcaga cucugacccc aguugggagg     1620

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1680

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1740

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1860

cucaacucau ugggcaaggg cauccaucaa auuauuggag cagcuuucaa aucauuguuu     1920

ggaggaaugu ccugguucuc acaaauucuc auugggacgu ugcugaugug guugggucug     1980

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2040

uuauccacag ccgucucagg u                                               2061


<210>  1040
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short CDS wild type

<400>  1040
ggagcugaug ccagcaucgg aaucgucagc cuccugcuga cuacagucau ggcagcagag       60

aucacuagac gcgggagugc auacuacaug uacuuggaca ggagcgaugc ugguaaggcc      120

auuucuuucg uuaccacacu gggggugaac aaaugccaug ugcagaucau ggaccucggg      180

cauaugugug acgccaccau gaguuaugag ugccccaugc uggacgaggg aguggagcca      240

gaugacgucg auugcuggug caacacgaca ucaacuuggg uuguguacgg aaccugucau      300

cauaaaaaag gugaagcacg acgauccaga agagccguga cgcuuccuuc ucacucuaca      360

aggaaguugc aaacgcgauc gcagacuugg cuagaaucaa gagaauacac aaagcaccug      420

aucaagguug agaauuggau auucaggaac cccggguuug cgcuaguggc uguagcuauu      480

gccuggcucc ugggaagcuc gacgagccaa aaagucauau acuuggucau gauauuguug      540

auugccccgg cauacaguau caggugcaua ggaguuagca auagagacuu cguggagggc      600

augucaggug ggaccugggu ugauguuguc uuggaacaug gagguugugu caccgugaug      660

gcacaggaca agccaacagu ugacauagag uuggucacga caacgguuag caacauggcc      720

gaggugagau ccuacugcua cgaggcauca auaucggaca uggcuucgga cagucgcugc      780

ccaacacaag gugaagccua ccuugacaag cagucagaca cucaauaugu cuguaaaaga      840

acauuggugg acagagguug gggaaauggg uguggacuuu uuggcaaggg gagcuuggug      900

acgugugcca aguuuacaug cuccaagaaa augacaggga agagcaucca gccggagaac      960

uuggaguacc ggauaaugcu aucagugcau ggaucccagc acagugggau gauugugaau     1020

gacgaaaaca gagcaaaagu cgagguuaca cccaauucac caagagcaga agcaaccuug     1080

ggagguuuug gaagccuggg acuugauugu gaaccaagga caggccuuga cuuuucagau     1140

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1200

aucccauuac cuuggcauuc uggugcagac acugaaacuc cacacuggaa caacaaagag     1260

gcacuggugg aguucaagga cgcccacgcc aagaggcaaa cuguuguggu ucuggggagc     1320

caagaaggag ccguucacac ggcucucgcu ggagcucugg aggcugagau ggauggugcg     1380

aagggaaggc uauccucagg ccauuugaaa ugccgccuaa aaauggacaa gcuuagguug     1440

aagggugugu cauauucccu guguaccgca gcguucacau ucaccaaggu uccagcugaa     1500

acauugcaug gaacagucac aguggaggug caguaugcag ggagggaugg acccugcaag     1560

gucccagccc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacg     1620

gcuaacccug ugaucacuga aagcacugag aauucaaaga ugauguugga gcucgaccca     1680

ccauuugggg auucuuacau ugucauagga gucggggaca agaaaaucac ccaucacugg     1740

caucggagug guagcaucau cggaaaggca uuugaagcca cugugagagg cgccaagaga     1800

auggcagucu ugggagacac agccugggac uuuggaucag uugggggugu guuuaacuca     1860

uugggcaagg guauucacca gaucuuugga gcagcuuuca aaucacuguu cggaggaaug     1920

uccugguucu cacagauccu cauaggcaca cuguuggugu gguugggucu gaacacaaag     1980

aauggaucua ucucccucac augcuuggcc uugggaggag ugaugaucuu ccuuuccacg     2040

gcuguuucug cu                                                         2052


<210>  1041
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short CDS wild type

<400>  1041
ggcgcagaca ccagcaucgg aaucauuggc cuccugcuga cuacagccau ggcagcagag       60

aucacuagac gcgggagugc auacuacaug uacuuggaua ggagcgaugc cgggaaggcc      120

auuucguuug cuaccacaau gggagugaac aagugccacg uacagaucau ggaccucggg      180

cacaugugug acgccaccau gaguuaugag ugcccuaugc uggaugaggg aguggaacca      240

gaugaugucg auugcuggug caacacgaca ucaacuuggg uuguguacgg aaccugucau      300

cacaaaaaag gugaggcacg gcccucaaga agagcuguca cgcuuccuuc ccauuccaca      360

agaaaguuac agacacgguc acaaaccugg cuugaaucac gggaguacac gaagcaccug      420

aucaaaguug agaacuggau acuccggaau ccaggguuca caguugcugc ucuagcaaug      480

gccuggcucu ugggaaauuc cacgagccag cgagugaucu aucuggugau gauccuguug      540

auugccccgg cauacaguau caggugcauu ggagucagca auagagacuu cguggagggc      600

augucaggug ggaccugggu ugauguuguc uuggaacaug gaggcugcgu uaccgugaug      660

gcacaggaca agccaacagu cgacauagag uuggucacga cgacgguuag uaacauggcc      720

gagguaagau ccuauugcua cgaggcaucg auaucggaca uggcuucgga cagucguugc      780

ccaacacaag gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga      840

acauuagugg acagagguug gggaaacggu uguggacuuu uuggcaaagg gagcuuggug      900

acaugugcca aguuuacgug uucuaagaag augaccggga agagcauuca accggagaau      960

uuggaguaca gaauuaugcu gucagugcac ggcucccaac acagcgggau gauugugaau     1020

gacauaggac augagaccga ugaaaacaga gcaaaagugg aggucacacc aaacucacca     1080

agagcugaag cgacccuagg gggcuucggg aguuugggac ucgauuguga gccaaggacc     1140

gguuuagauu ucuccgaccu guacuauuua accaugaaca acaagcauug guuggugcac     1200

aaggaguggu uccacgacau uccauugcca uggcacgcug gagcggacac agggacuccc     1260

cauuggaaca acaaagaggc auugguugag uucaaggaug cccacgccaa aagacagacu     1320

guugucgugu ugggaaguca ggagggugcc guccacacag cccuggcagg agcuuuggaa     1380

gcggagaugg auggagccaa aggaaggcug uucucagguc auuugaagug ccggauaaag     1440

auggacaagc uuagguugaa gggugucuca uacucauugu gcacugccgc cuucacauuc     1500

ucaaagguuc cggcugaaac gcugcauggg acagucacag uugaggugca guaugcgggc     1560

agagauggac cuugcaaggu gccagcccaa auggcagugg auaugcagac ucuuacccca     1620

gugggaaggu ugauaacggc aaaucccauc auaacugaga gcacugaaaa cucgagaaug     1680

auguuggagc uggacccacc cuuuggagac ucuuacauug ucauuggggu uggagauaag     1740

aaaauuaccc aucauuggca cagaagugga agcacgauug ggaaggcauu ugaggccacu     1800

gugagaggug caaaaaggau ggcaguccug ggagauaccg ccugggauuu ugguucuguu     1860

ggaggcgugu ucaauucucu gggcaaggga guucaucagg ucuucggagc agccuuuaaa     1920

uccuuauuug gcggaauguc cugguuuuca caaauucuua uuggcacacu uuuagugugg     1980

uugggcuuga auacaaaaaa uggauccauu ucccuugcau gucuggcgcu uggaggggcg     2040

augaucuuuc ucuccacggc cgucucggca                                      2070


<210>  1042
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE CDS wild type

<400>  1042
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1043
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE CDS wild type

<400>  1043
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacu guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1044
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE CDS wild type

<400>  1044
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      180

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      360

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      420

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca      480

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa      540

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaagcuua      600

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug      660

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau      720

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauucaag      780

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac      840

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu      900

ggccauuuga aaugccgccu aaaaauggac aagcuuagau ugaagggcgu gucauauucc      960

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1020

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1080

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1140

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1200

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1260

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1320

acagccuggg acuucggauc a                                               1341


<210>  1045
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE CDS wild type

<400>  1045
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guccacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1046
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE CDS wild type

<400>  1046
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      180

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      360

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      420

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca      480

gugcauggcu cccagcauag cgggaugauu gucaaugaua caggauauga aacugacgaa      540

aauagagcga aagucgaggu uacgccuaau ucaccaagag cggaagcaac cuugggaggc      600

uuuggaagcu uaggacuuga cugugaacca aggacaggcc uugacuuuuc agaucuguau      660

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca      720

uugccuuggc augcuggggc agacaccgga acuccacacu ggaacaacaa agaggcauug      780

guagaauuca aggaugccca cgccaagagg caaaccgucg ucguucuggg gagccaggaa      840

ggagccguuc acacggcucu cgcuggagcu cuagaggcug agauggaugg ugcaaaggga      900

aagcuguucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag auugaagggc      960

gugucauauu ccuugugcac ugcggcauuc acauucacca aggucccagc ugaaacacug     1020

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagauccca     1080

guccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaac     1140

cccgugauua cugaaagcac ugagaacuca aagaugaugu uggagcuuga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccacca cuggcauagg     1260

agugguagca ccaucggaaa ggcauuugag gccacuguga gaggcgccaa gagaauggca     1320

guccuggggg auacagccug ggacuucgga uca                                  1353


<210>  1047
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE CDS wild type

<400>  1047
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacaguugac      180

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      360

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      420

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca      480

gugcauggcu cccagcauag cgggaugacu gucaaugaua uaggauauga aacugacgaa      540

aauagagcga aagucgaggu uacgccuaau ucaccaagag cggaagcaac cuugggaggc      600

uuuggaagcu uaggacuuga cugugaacca aggacaggcc uugacuuuuc agaucuguau      660

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca      720

uugccuuggc augcuggggc agacacugga acuccacacu ggaacaacaa agaggcauug      780

guagaauuca aggaugccca cgccaagagg caaaccgucg ucguucuggg gagccaggaa      840

ggagccguuc acacggcucu cgcuggagcu cuagaggcug agauggaugg ugcaaaggga      900

aagcuguucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag auugaagggc      960

gugucauauu ccuugugcac ugcggcauuc acauucacca aggucccagc ugaaacacug     1020

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagauccca     1080

guccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaac     1140

cccgugauua cugaaagcac ugagaacuca aagaugaugu uggagcuuga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccacca cuggcauagg     1260

agugguagca ccaucggaaa ggcauuugag gccacuguga gaggcgccaa gagaauggca     1320

guccuggggg auacagccug ggacuucgga uca                                  1353


<210>  1048
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE CDS wild type

<400>  1048
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauaggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugac      120

guuguccugg aacauggagg cugcgucacc gugauggcac aggacaagcc aacaguugac      180

auagagcugg ucacaacaac gguuaguaac auggccgagg ugagauccua uuguuacgag      240

gcaucaauau cggacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auauguuugc aaaagaacau ugguggacag agguugggga      360

aaugggugug gacucuuugg caaagggagu uuggugacau gugcuaaguu cacgugcucc      420

aagaagauga cugggaagag cauucagccg gagaaccugg aguaucggau aaugcuauca      480

gugcauggcu cccagcacag ugggaugauu guuaaugaug aaaacagagc gaaggucgag      540

guuacgccca auucaccaag agcagaagca acccugggag gcuuuggaag cuuaggacuu      600

gauugugaac caaggacagg ccuugacuuu ucagaucugu auuaccuaac caugaauaac      660

aagcauuggu uggugcacaa agagugguuu caugacaucc cauugcccug gcaugcuggg      720

gcagacacug gaacuccaca uuggaacaac aaggaggcau uaguggaauu caaggacgcc      780

cacgccaaga ggcaaaccgu cgugguuuug gggagccagg aaggagccgu ccacacggcu      840

cuugcuggag cucuagaggc ugagauggau ggugcaaagg gaaggcuauu cucuggccac      900

uugaaauguc gcuuaaaaau ggacaagcuu agauugaagg gcgugucaua uuccuugugc      960

accgcggcau ucacauucac caaggucccg gcugaaacac uacauggaac agucacagug     1020

gaggugcagu augcagggac agauggaccc ugcaaggucc cagcccagau ggcgguggac     1080

augcagaccu ugaccccagu cggaaggcug auaaccgcca accccgugau uacugaaagc     1140

acugagaauu caaagaugau guuggagcuc gacccaccau uuggggauuc uuacauuguc     1200

auaggaguug gggauaagaa aaucacccau cacuggcaua ggaguggcag caccauugga     1260

aaagcauuug aagccacugu gagaggcgcu aagagaaugg caguccuggg ggacacagcu     1320

ugggacuuug gauca                                                      1335


<210>  1049
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE CDS wild type

<400>  1049
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      180

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      360

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      420

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaccggau aaugcuauca      480

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaaa uagagcgaaa      540

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaagcuua      600

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug      660

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau      720

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauucaag      780

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac      840

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaaa gcuguucucu      900

ggccauuuga aaugccgccu aaaaauggac aagcuuagau ugaagggcgu gucauauucc      960

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1020

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1080

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1140

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1200

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1260

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1320

acagccuggg acuucggauc a                                               1341


<210>  1050
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE CDS wild type

<400>  1050
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagccucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucugacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1051
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE CDS wild type

<400>  1051
agcaaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1052
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE CDS wild type

<400>  1052
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cugguacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1053
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE CDS wild type

<400>  1053
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aagcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1054
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE CDS wild type

<400>  1054
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaauaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacac gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1055
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE CDS wild type

<400>  1055
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggaga uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guggaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1056
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE CDS wild type

<400>  1056
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucc aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccag gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1057
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE CDS wild type

<400>  1057
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggu uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuucuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1058
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE CDS wild type

<400>  1058
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua uagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac cuuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcau ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1059
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE CDS wild type

<400>  1059
agccaaaaag ucauauauuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacucag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggaaucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1060
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE CDS wild type

<400>  1060
agccaaaaag ucauauacuu ggucaugaua cugcugauug cccuggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aauguuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcccagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1061
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE CDS wild type

<400>  1061
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggggg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcu uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gacucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1062
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE CDS wild type

<400>  1062
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucua     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1063
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE CDS wild type

<400>  1063
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcu uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggaagcaca ugcuaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1064
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE CDS wild type

<400>  1064
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccgc ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

ccuguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1065
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE CDS wild type

<400>  1065
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuucaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaacaauau cagacauagc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugccg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1066
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE CDS wild type

<400>  1066
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucucca agaucccggc ugaaacacug     1020

cacgggacag ucacagagga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

ccgguaauca cugaaagcac agagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1067
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE CDS wild type

<400>  1067
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucugacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga aaguugagau aacgcccaau ucaccgagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga guuacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1068
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE CDS wild type

<400>  1068
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggaa ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca guaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaaucc uaggacugga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1069
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE CDS wild type

<400>  1069
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg uguaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aaaaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuuaggag acacagccug ggacuuugga uca                                  1353


<210>  1070
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE CDS wild type

<400>  1070
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu ugguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcauugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcaggggcag auggacccug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgccaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1071
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE CDS wild type

<400>  1071
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucaguaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaacaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acacuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaggcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1072
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE CDS wild type

<400>  1072
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc gcaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auauguuugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugcuc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug gcucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agccaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacagug     1020

gacgggacag ucacagugga gggacaguac ggagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcagacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1073
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE CDS wild type

<400>  1073
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uuguguuacc guaauggcac aggacaaacc ggcugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua uugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcagu cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag cugaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc augcuggggc agacaccgga acuccacauu ggaacaacaa agaagcauug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggacccug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggaggcugau aaccgcuaac     1140

ccuguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccauca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggauuuugga uca                                  1353


<210>  1074
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE CDS wild type

<400>  1074
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua uagcaucagg       60

ugcauaggag ucaacaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

gucgucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcagagg uaagauccua cugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgccaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccgaau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccugggaggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggaugcaca ugccaaaagg caaacugucg ugguccuagg gagucaagag      840

ggagcaguuc acacggcucu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag acugaagggc      960

gugucauacu cccuguguac cgcagcguuc acauucacua agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

ccuguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1075
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE CDS wild type

<400>  1075
agccaaaaag ucauauauuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg cuguguuacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac aguuagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cggauauggc uucggacagc cgcugcccaa cacaagguga ggccuaccuu      300

gacaagcagu cagacacuca auaugucugc aaaagaacgu uaguggauag aggcugggga      360

aauggaugug gacuuuuugg aaaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaccugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uaccugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc augcuggggc agacacugga acuccacauu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacucag auugaagggu      960

gugucauacu ccuugugugc ugcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1080

gcucagaugg cgguggauau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

ccuguaauca cugaaagcac cgagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1076
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE CDS wild type

<400>  1076
agccaaaaag ucauauacuu ggucaugaua cuguugauug ccccggcaua caguaucagg       60

ugcauaggag ucagcaauag ggauuuugug gaagguaugu caggugggac cuggguugau      120

guugucuugg aacauggagg uuguguuacc guaauggcac aggacaagcc aacuguugau      180

auagaguugg ucacaacaac gguuagcaac auggcggagg uaagauccua cugcuacgag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcagu cagacacuca auauguuugc aaaagaacgu uaguggacag agguugggga      360

aauggaugug gacucuuugg caaagggagc cuggugacau gcgccaaguu ugcaugcucc      420

aagaaaauga cugggaagag cauccagcca gagaaccugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauu guuaaugaca uaggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccugggaggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcauugguug gugcacaagg agugguucca ugacauucca      720

cuaccuuggc augcuggggc agacaccgga acuccacauu ggaacaacaa agaagcauug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagccguuc acacggcucu ugcuggagcc cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc uugaaaaugg acaaacuuag auugaagggc      960

gugucauacu ccuuauguac cgcggcguuc acauucacca agaucccggc ugaaacgcug     1020

caugggacag ucacagugga gguacaguau gcagggacag auggacccug caagguucca     1080

gcucagaugg cgguggauau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

ccugugauca cugaaagcac ugagaauuca aagaugaugu uggaacuuga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gauaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccaucggaaa agcauuugaa gccacuguga gaggcgccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1077
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE CDS wild type

<400>  1077
agccaaaaag ucauauacuu ggucaugaua cuguugauug ccccggcaua caguaucagg       60

ugcauaggag ucagcaauag ggauuuugug gaagguaugu caggugggac cuggguugau      120

guugucuugg aacauggagg uuguguuacc guaauggcac aggacaagcc aacuguugau      180

auagaguugg ucacaacaac gguuagcaac auggcggagg uaagauccua cugcuacgag      240

gcaucaauau cggacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcagu cagacacuca auauguuugc aaaagaacgu uaguggacag agguugggga      360

aauggaugug gacucuuugg caaagggagc cuggugacau gcgccaaguu ugcaugcucc      420

aagaaaauga cugggaagag cauccagcca gagaaccugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauu guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccugggaggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcauugguug gugcacaagg agugguucca ugacauucca      720

cuaccuuggc augcuggggc agacaccgga acuccacauu ggaacaacaa agaagcauug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagccguuc acacggcucu ugcuggagcc cuggaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc uugaaaaugg acaaacuuag auugaagggc      960

gugucauacu ccuuauguac cgcggcguuc acauucacca agaucccggc ugaaacgcug     1020

caugggacag ucacagugga gguacaguau gcagggacag auggacccug caagguucca     1080

gcucagaugg cgguggauau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

ccugugauca cugaaagcac ugagaauuca aagaugaugu uggaacuuga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gauaagaaga ucacccacca cugguacagg     1260

aguggcagca ccaucggaaa agcauuugaa gccacuguga gaggcgccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1078
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE CDS wild type

<400>  1078
agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg       60

ugcauaggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugac      120

guuguccugg aacauggagg cugcgucacc gugauggcac aggacaagcc aacaguugac      180

auagagcugg ucacaacaac gguuaguaac auggccgagg ugagauccua uuguuacgag      240

gcaucaauau cggacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auauguuugc aaaagaacau ugguggacag agguugggga      360

aaugggugug gacucuuugg caaagggagu uuggugacau gugcuaaguu cacgugcucc      420

aagaagauga cugggaagag cauucagccg gagaaccugg aguaucggau aaugcuauca      480

gugcauggcu cccagcacag ugggaugauu guuaaugaca caggacauga aacugaugaa      540

aacagagcga aggucgaggu uacgcccaau ucaccaagag cagaagcaac ccugggaggc      600

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau      660

uaccuaacca ugaauaacaa gcauugguug gugcacaaag agugguuuca ugacauccca      720

uugcccuggc augcuggggc agacacugga acuccacauu ggaacaacaa ggaggcauua      780

guggaauuca aggacgccca cgccaagagg caaaccgucg ugguuuuggg gagccaggaa      840

ggagccgucc acacggcucu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga      900

aggcuauucu cuggccacuu gaaaugucgc uuaaaaaugg acaagcuuag auugaagggc      960

gugucauauu ccuugugcac cgcggcauuc acauucacca aggucccggc ugaaacacua     1020

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1080

gcccagaugg cgguggacau gcagaccuug accccagucg gaaggcugau aaccgccaac     1140

cccgugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gauaagaaaa ucacccauca cuggcauagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggcgcuaa gagaauggca     1320

guccuggggg acacagcuug ggacuuugga uca                                  1353


<210>  1079
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE CDS wild type

<400>  1079
agccaaaaag ucauauacuu gguuaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauuggag ucagcaauag agacuucgug gaaggcaugu caggugggac cuggguugau      120

gucgucuugg aacauggagg uugcgucacc guuauggcac aggacaagcc aacaguugac      180

auagaguugg ucacgacaac gguuaguaac auggccgagg uaagauccua cuguuaugag      240

gcaucaauau cagacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      300

gauaagcaau cagacaccca auaugugugc aaaagaacau ugguggacag agguugggga      360

aaugggugug gacuuuuugg caaagggagc cuggugacau gugccaaguu cacguguucc      420

aagaagauga cugggaagag cauucagccg gagaaucugg aguaucgaau aaugcuauca      480

gugcauggcu cccagcacag cgggaugauu guuaaugaca uaggacauga aacugacgaa      540

aacagagcga aagucgaggu cacgcccaau ucaccaagag cagaagcaac cuugggaggc      600

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uggacuuuuc agauuuguac      660

uacuugacca ugaacaacaa gcacugguug gugcacaaag agugguuuca ugacauccca      720

uugccuuggc augcuggggc agauaccgga acuccacacu ggaacaacaa ggaggcauug      780

guagaguuca aagaugccca cgccaagagg caaaccgucg uuguucuagg gagccaggaa      840

ggagccguuc acacggcucu ugcuggagcu cuggaggcug agauggaugg ugcaaaagga      900

aggcuguucu ccggccauuu gaaaugccgc uuaaaaaugg acaagcuuag auugaagggc      960

gugucauauu ccuugugcac cgcagcguuc acauuuacca aggucccagc ugaaacauug     1020

cauggaacag uuacagugga ggugcaguau gcagggacag auggacccug caagguccca     1080

gcccagaugg cgguggacau gcagacccug accccagucg ggaggcugau aaccgccaac     1140

ccugugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccauca cuggcauagg     1260

agugguagca ccaucggaaa agcauuugag gccacuguga gaggugccaa gagaauggca     1320

guucuggggg auacagccug ggacuucgga uca                                  1353


<210>  1080
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE CDS wild type

<400>  1080
agccaaaaag ucauauacuu gguaaugaua uuguugauug ccccggcaua cagcaucagg       60

ugcauaggag uuagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggggg uugcgucacc gugauggcac aggacaagcc aacaguugac      180

aucgaguugg ucacgacaac gguuagcaac auggccgagg uaagauccua cugcuaugag      240

gcaucaauau cggacauggc uucggacagc cgcuguccaa cacaagguga agccuaccuu      300

gacaagcagu cagacacuca auaugucugc aagagaacau ugguggauag agguugggga      360

aaugggugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacgugcucc      420

aagaaaauga caggcaagag cauccagccg gagaacuugg aguaccggau aaugcuauca      480

gugcauggau cccagcacag ugggaugauu gugaaugaca uaggacauga aacugacgaa      540

aacagagcaa aagucgaggu cacacccaau ucaccaagag cagaagcaac cuugggaggu      600

uuuggaagcu ugggacuuga cugugaacca aggacaggcc uugacuucuc agaucuguau      660

uaccugacca ugaacaauaa gcauugguug gugcacaagg agugguuuca ugacauccca      720

uuaccuuggc augcuggugc agacacugga acuccacacu ggaacaacaa agaggcauug      780

guggaguuua aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagag      840

ggagcuguuc acacggcccu cgcuggagcu uuggaggcug agauggaugg ugcaaaggga      900

aggcuauucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu      960

gugucauauu cccuguguac cgcagugugu accgcagcga aggucccagc ugaaacauug     1020

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1080

gcccagaugg cgguagacau gcagacccug accccaguug gaaggcugau aaccgccaac     1140

ccugugauca cugaaaguac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1200

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1260

agugguagca ccaucggaaa ggcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuuggggg acacagccug ggacuuugga uca                                  1353


<210>  1081
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE CDS wild type

<400>  1081
agccaaaaag ucauauacuu ggucaugaua uuguugauug ccccggcaua caguaucagg       60

ugcauaggag uuagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg uugugucacc gugauggcac aggacaagcc aacaguugac      180

auagaguugg ucacgacaac gguuagcaac auggccgagg ugagauccua cugcuacgag      240

gcaucaauau cggacauggc uucggacagu cgcugcccaa cacaagguga agccuaccuu      300

gacaagcagu cagacacuca auaugucugu aaaagaacau ugguggacag agguugggga      360

aaugggugug gacuuuuugg caaggggagc uuggugacgu gugccaaguu uacaugcucc      420

aagaaaauga cagggaagag cauccagccg gagaacuugg aguaccggau aaugcuauca      480

gugcauggau cccagcacag ugggaugauu gugaaugacg aaaacagagc aaaagucgag      540

guuacaccca auucaccaag agcagaagca accuugggag guuuuggaag ccugggacuu      600

gauugugaac caaggacagg ccuugacuuu ucagaucugu auuaccugac caugaacaau      660

aagcauuggu uggugcacaa agagugguuu caugacaucc cauuaccuug gcauucuggu      720

gcagacacug aaacuccaca cuggaacaac aaagaggcac ugguggaguu caaggacgcc      780

cacgccaaga ggcaaacugu ugugguucug gggagccaag aaggagccgu ucacacggcu      840

cucgcuggag cucuggaggc ugagauggau ggugcgaagg gaaggcuauc cucaggccau      900

uugaaaugcc gccuaaaaau ggacaagcuu agguugaagg gugugucaua uucccugugu      960

accgcagcgu ucacauucac caagguucca gcugaaacau ugcauggaac agucacagug     1020

gaggugcagu augcagggag ggauggaccc ugcaaggucc cagcccagau ggcgguggac     1080

augcagaccc ugaccccagu uggaaggcug auaacggcua acccugugau cacugaaagc     1140

acugagaauu caaagaugau guuggagcuc gacccaccau uuggggauuc uuacauuguc     1200

auaggagucg gggacaagaa aaucacccau cacuggcauc ggagugguag caucaucgga     1260

aaggcauuug aagccacugu gagaggcgcc aagagaaugg cagucuuggg agacacagcc     1320

ugggacuuug gauca                                                      1335


<210>  1082
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE CDS wild type

<400>  1082
agccaaaaag ucauauacuu ggucaugaua guguugauug ucccggcaua caguaucagc       60

ugcauuggag ucagcaauag agacuuagug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg gugcguuacc gagauggcac aggacaagcc aacaguugac      180

auagaguugg ucacgaugac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucguuau ccgacauggc uucggccagu cguugcccaa cacaaggcga acccucccuc      300

gacaagcaau cagacacuca aucuguaugc aaaagaacau uaggagacag agguugggga      360

aaugguugug ggauuuuugg caaagggagc uuggugacau guuccaaguu cacguguugu      420

aagaagaugc ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuccca      480

gugcauggcu cccagcauag cgggaugauu gugaaugaca uaggacauga aacugacgaa      540

aacagagcga aagucgaggu cacacccaau ucaccaagag cagaagcaac cuugggaggu      600

uuuggaagcu ugggacuuga cugugaacca aggacaggcc uugacuucuc agaucuguau      660

uaucugacca ugaacaacaa gcauugguug gugcacaagg agugguuuca ugacauccca      720

uuaccuuggc augcuggugc agacacugga acuccacacu ggaacaacaa agaggcauug      780

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagag      840

ggagcuguuc acacggcccu cgcuggagcu cuggaggcug agauggaugg ugcaaaggga      900

aggcuauucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu      960

gugucauauu cccuguguac ugcagcguuc acauuuacca aggucccagc ugaaacauug     1020

cauggaacag uuacagugga ggugcagucu gcagggacag auggacccug caagguccca     1080

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaac     1140

cccgugauua cugaaagcac ugagaacuca aagaugaugu uggagcuuga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gacaagaaaa ucacccacca cuggcauagg     1260

agugguagca ccaucggaaa ggcauuugaa gccacuguga gaggcgccaa gagaauggca     1320

guccuggggg auacagccug ggacuucgga uca                                  1353


<210>  1083
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE CDS wild type

<400>  1083
cgccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg       60

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      120

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      180

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      240

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      360

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      420

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      480

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      540

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      600

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      660

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      720

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug      780

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa      840

ggagcaguuc acacggcccu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga      900

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc      960

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1020

cacgggacag ucacagugga gguacaguac gcagggacug auggaccuug caagguucca     1080

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1140

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1200

ggggacuccu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga uca                                  1353


<210>  1084
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE CDS wild type

<400>  1084
agccagcgag ugaucuaucu ggugaugauc cuguugauug ccccggcaua caguaucagg       60

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      120

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      180

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      240

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      300

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      360

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      420

aagaagauga ccgggaagag cauucaaccg gagaauuugg aguacagaau uaugcuguca      480

gugcacggcu cccaacacag cgggaugauu gugaaugaca uaggacauga gaccgaugaa      540

aacagagcaa aaguggaggu cacaccaaac ucaccaagag cugaagcgac ccuagggggc      600

uucgggaguu ugggacucga uugugagcca aggaccgguu uagauuucuc cgaccuguac      660

uauuuaacca ugaacaacaa gcauugguug gugcacaagg agugguucca cgacauucca      720

uugccauggc acgcuggagc ggacacaggg acuccccauu ggaacaacaa agaggcauug      780

guugaguuca aggaugccca cgccaaaaga cagacuguug ucguguuggg aagucaggag      840

ggugccgucc acacagcccu ggcaggagcu uuggaagcgg agauggaugg agccaaagga      900

aggcuguucu caggucauuu gaagugccgg auaaagaugg acaagcuuag guugaagggu      960

gucucauacu cauugugcac ugccgccuuc acauucucaa agguuccggc ugaaacgcug     1020

caugggacag ucacaguuga ggugcaguau gcgggcagag auggaccuug caaggugcca     1080

gcccaaaugg caguggauau gcagacucuu accccagugg gaagguugau aacggcaaau     1140

cccaucauaa cugagagcac ugaaaacucg agaaugaugu uggagcugga cccacccuuu     1200

ggagacucuu acauugucau ugggguugga gauaagaaaa uuacccauca uuggcacaga     1260

aguggaagca cgauugggaa ggcauuugag gccacuguga gaggugcaaa aaggauggca     1320

guccugggag auaccgccug ggauuuuggu ucu                                  1353


<210>  1085
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS GC

<400>  1085
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cuccgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaag                               2136


<210>  1086
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS GC

<400>  1086
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cuccgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca cgugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accacgagca ccugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacgcgca agcugcagac ccggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacg      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac cuggguggac      660

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      720

aucgagcucg ugacgaccac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacacgca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca cgggccacga gaccgacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggacgggcc uggacuucag cgaccuguac     1200

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg cucccaggag     1380

ggggccgugc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacgcuc     1560

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1620

gcccagaugg ccguggacau gcagacgcug acccccgucg ggcgccugau caccgcgaac     1680

cccgugauca ccgagagcac ggagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacccacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1860

gugcuggggg acacggcgug ggacuucggc agcgucggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacccugcug auguggcucg ggcugaacgc caagaacggc     2040

uccaucagcc ugaugugccu cgcgcugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1087
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS GC

<400>  1087
cggaucauca acgcccgcaa ggagcggaag cgccggggcg ccgacaccag caucgggauc       60

aucggccugc ugcucacgac cgccauggcg gccgagauca cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc aaggccaucu ccuucgcgac cacgcugggc      180

gugaacaagu gccacgugca gaucauggac cucgggcaca ugugcgacgc caccaugagc      240

uacgagugcc cgaugcugga cgagggcguc gagcccgacg acguggacug cuggugcaac      300

accaccucca cguggguggu cuacgggacc ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgugacccu gcccucccac agcacccgga agcuccagac gcgcucccag      420

accuggcugg agagccgcga guacaccaag caccugauca agguggagaa cuggaucuuc      480

cggaaccccg gcuucgcccu cgucgcggug gccaucgccu ggcugcuggg guccagcacc      540

ucccagaagg ugaucuaccu cgucaugauc cugcugaucg ccccggccua cagcauccgc      600

ugcaucggcg uguccaaccg ggacuucgug gaggggauga gcggcgggac gugggucgac      660

guggugcucg agcacggcgg cugcgucacc gugauggcgc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccagcaucu ccgacauggc cagcgacucc cggugcccca cccaggggga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcacgc ucguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caagggcucc cuggucacgu gcgcgaaguu cacgugcagc      960

aagaagauga ccgggaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc ggcuacgaga cggacgagga ccgggccaag     1080

guggagguca ccccgaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccuc     1140

gggcuggacu gcgagccccg gaccggccug gacuucuccg accucuacua ccugacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu cccguggcac     1260

gcgggggccg acaccggcac cccgcacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagcgcca gaccgucgug gugcugggca gccaggaggg ggcgguccac     1380

acggcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggccg gcuguucucc     1440

gggcaccuga agugccgccu caagauggac aagcugcggc ugaagggcgu gagcuacucc     1500

cucugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggccccugca agaucccggu ccagauggcg     1620

guggacaugc agacccugac gcccgugggg cgccucauca ccgccaaccc cgucaucacc     1680

gagagcaccg agaacuccaa gaugaugcug gagcuggacc cgccguucgg cgacagcuac     1740

aucgugaucg gggugggcga caagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgcgaagc ggauggccgu gcucggggac     1860

accgccuggg acuucggcag cgugggcggc gucuucaacu cccuggggaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggga cgcugcuggu guggcucggc cugaacacca agaacggcuc caucagccug     2040

accugccucg cgcugggcgg cgugaugauc uuccugucca ccgccgucag cgccgacgug     2100

gggugcuccg uggacuucag caag                                            2124


<210>  1088
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) CDS GC

<400>  1088
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cuccgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      720

aucgagcucg ugaccacgac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

guggaguuca aggacgcgca cgccaagcgc cagaccgugg ucgugcuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgucg ggcgccugau caccgcgaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgucggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgcggug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1089
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS GC

<400>  1089
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1090
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) CDS GC

<400>  1090
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacacgugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac gagcaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac gcgcaagcug      360

cagacccgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccacgagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacg accaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acgcaguacg ucugcaagcg cacccuggug      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugaa cgacacgggc     1020

cacgagaccg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac gggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac gguggucgug     1320

cugggcuccc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cgcuccacgg caccgugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cgcugacccc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcacggaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccgugcu gggggacacg gcgugggacu ucggcagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacgccaaga acggcuccau cagccugaug ugccucgcgc ugggcggcgu gcugaucuuc     2040

cucuccaccg ccgugagcgc c                                               2061


<210>  1091
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) CDS GC

<400>  1091
accagcaucg ggaucaucgg ccugcugcuc acgaccgcca uggcggccga gaucacccgc       60

cggggguccg ccuacuacau guaccuggac cgcagcgacg ccggcaaggc caucuccuuc      120

gcgaccacgc ugggcgugaa caagugccac gugcagauca uggaccucgg gcacaugugc      180

gacgccacca ugagcuacga gugcccgaug cuggacgagg gcgucgagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccacgugg guggucuacg ggaccugcca ccacaagaag      300

ggcgaggccc ggcgcagccg gcgcgccgug acccugcccu cccacagcac ccggaagcuc      360

cagacgcgcu cccagaccug gcuggagagc cgcgaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuuccggaa ccccggcuuc gcccucgucg cgguggccau cgccuggcug      480

cuggggucca gcaccuccca gaaggugauc uaccucguca ugauccugcu gaucgccccg      540

gccuacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

gggacguggg ucgacguggu gcucgagcac ggcggcugcg ucaccgugau ggcgcaggac      660

aagcccaccg uggacaucga gcuggucacc acgaccgugu ccaacauggc cgagguccgc      720

agcuacugcu acgaggccag caucuccgac auggccagcg acucccggug ccccacccag      780

ggggaggccu accuggacaa gcagagcgac acccaguacg ugugcaagcg cacgcucgug      840

gaccggggcu gggggaacgg cugcgggcug uucggcaagg gcucccuggu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ugagcgugca cggcucccag cacagcggga ugaucggcua cgagacggac     1020

gaggaccggg ccaaggugga ggucaccccg aacuccccgc gcgccgaggc cacccugggc     1080

ggguucggca gccucgggcu ggacugcgag ccccggaccg gccuggacuu cuccgaccuc     1140

uacuaccuga cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccucccgu ggcacgcggg ggccgacacc ggcaccccgc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag cgccagaccg ucguggugcu gggcagccag     1320

gagggggcgg uccacacggc ccuggccggc gcccucgagg ccgagaugga cggggcgaag     1380

ggccggcugu ucuccgggca ccugaagugc cgccucaaga uggacaagcu gcggcugaag     1440

ggcgugagcu acucccucug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc cugcaagauc     1560

ccgguccaga uggcggugga caugcagacc cugacgcccg uggggcgccu caucaccgcc     1620

aaccccguca ucaccgagag caccgagaac uccaagauga ugcuggagcu ggacccgccg     1680

uucggcgaca gcuacaucgu gaucggggug ggcgacaaga agaucacgca ccacuggcac     1740

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc gaagcggaug     1800

gccgugcucg gggacaccgc cugggacuuc ggcagcgugg gcggcgucuu caacucccug     1860

gggaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1920

ugguucagcc agauccucau cgggacgcug cugguguggc ucggccugaa caccaagaac     1980

ggcuccauca gccugaccug ccucgcgcug ggcggcguga ugaucuuccu guccaccgcc     2040

gucagcgcc                                                             2049


<210>  1092
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) CDS GC

<400>  1092
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacc acgaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacgcuggug      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac cguggucgug     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cccugacgcc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu gcugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1093
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS GC

<400>  1093
agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc       60

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      120

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      180

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      300

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      360

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      420

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      480

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      600

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      660

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg      720

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag      840

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc      900

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg      960

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1020

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1080

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1140

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1320

gugcuggggg acaccgcgug ggacuucggc agc                                  1353


<210>  1094
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS GC

<400>  1094
agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc       60

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac cuggguggac      120

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      180

aucgagcucg ugacgaccac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      300

gacaagcagu ccgacacgca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      420

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      480

guccacggca gccagcacuc cgggaugauc gugaacgaca cgggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      600

uucggcuccc uggggcucga cugcgagccc cggacgggcc uggacuucag cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg      720

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg cucccaggag      840

ggggccgugc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc      900

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg      960

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacgcuc     1020

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1080

gcccagaugg ccguggacau gcagacgcug acccccgucg ggcgccugau caccgcgaac     1140

cccgugauca ccgagagcac ggagaacagc aagaugaugc ucgagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacccacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1320

gugcuggggg acacggcgug ggacuucggc agc                                  1353


<210>  1095
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS GC

<400>  1095
ucccagaagg ugaucuaccu cgucaugauc cugcugaucg ccccggccua cagcauccgc       60

ugcaucggcg uguccaaccg ggacuucgug gaggggauga gcggcgggac gugggucgac      120

guggugcucg agcacggcgg cugcgucacc gugauggcgc aggacaagcc caccguggac      180

aucgagcugg ucaccacgac cguguccaac auggccgagg uccgcagcua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cccaggggga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcacgc ucguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caagggcucc cuggucacgu gcgcgaaguu cacgugcagc      420

aagaagauga ccgggaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc      480

gugcacggcu cccagcacag cgggaugauc ggcuacgaga cggacgagga ccgggccaag      540

guggagguca ccccgaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccuc      600

gggcuggacu gcgagccccg gaccggccug gacuucuccg accucuacua ccugacgaug      660

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu cccguggcac      720

gcgggggccg acaccggcac cccgcacugg aacaacaagg aggcccuggu ggaguucaag      780

gacgcccacg ccaagcgcca gaccgucgug gugcugggca gccaggaggg ggcgguccac      840

acggcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggccg gcuguucucc      900

gggcaccuga agugccgccu caagauggac aagcugcggc ugaagggcgu gagcuacucc      960

cucugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcacgguc     1020

accguggagg ugcaguacgc cgggaccgac ggccccugca agaucccggu ccagauggcg     1080

guggacaugc agacccugac gcccgugggg cgccucauca ccgccaaccc cgucaucacc     1140

gagagcaccg agaacuccaa gaugaugcug gagcuggacc cgccguucgg cgacagcuac     1200

aucgugaucg gggugggcga caagaagauc acgcaccacu ggcaccggag cggcuccacc     1260

aucgggaagg ccuucgaggc caccguccgc ggcgcgaagc ggauggccgu gcucggggac     1320

accgccuggg acuucggcag c                                               1341


<210>  1096
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) CDS GC

<400>  1096
agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc       60

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      120

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      180

aucgagcucg ugaccacgac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      300

gacaagcagu ccgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      360

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      420

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      480

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gacggacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      600

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      660

uaccucacga ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg      720

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgcgca cgccaagcgc cagaccgugg ucgugcuggg cucccaggag      840

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc      900

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg      960

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1020

cacggcacgg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acgcccgucg ggcgccugau caccgcgaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacgcacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1320

gugcuggggg acaccgcgug ggacuucggc agc                                  1353


<210>  1097
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) CDS GC

<400>  1097
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uccgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1098
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) CDS GC

<400>  1098
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacacgugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac gagcaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac gcgcaagcug      360

cagacccgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccacgagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacg accaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acgcaguacg ucugcaagcg cacccuggug      840

gaccggggcu gggggaacgg cugcggccuc uccgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugaa cgacacgggc     1020

cacgagaccg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac gggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac gguggucgug     1320

cugggcuccc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cgcuccacgg caccgugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cgcugacccc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcacggaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccgugcu gggggacacg gcgugggacu ucggcagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacgccaaga acggcuccau cagccugaug ugccucgcgc ugggcggcgu gcugaucuuc     2040

cucuccaccg ccgugagcgc c                                               2061


<210>  1099
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) CDS GC

<400>  1099
accagcaucg ggaucaucgg ccugcugcuc acgaccgcca uggcggccga gaucacccgc       60

cggggguccg ccuacuacau guaccuggac cgcagcgacg ccggcaaggc caucuccuuc      120

gcgaccacgc ugggcgugaa caagugccac gugcagauca uggaccucgg gcacaugugc      180

gacgccacca ugagcuacga gugcccgaug cuggacgagg gcgucgagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccacgugg guggucuacg ggaccugcca ccacaagaag      300

ggcgaggccc ggcgcagccg gcgcgccgug acccugcccu cccacagcac ccggaagcuc      360

cagacgcgcu cccagaccug gcuggagagc cgcgaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuuccggaa ccccggcuuc gcccucgucg cgguggccau cgccuggcug      480

cuggggucca gcaccuccca gaaggugauc uaccucguca ugauccugcu gaucgccccg      540

gccuacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

gggacguggg ucgacguggu gcucgagcac ggcggcugcg ucaccgugau ggcgcaggac      660

aagcccaccg uggacaucga gcuggucacc acgaccgugu ccaacauggc cgagguccgc      720

agcuacugcu acgaggccag caucuccgac auggccagcg acucccggug ccccacccag      780

ggggaggccu accuggacaa gcagagcgac acccaguacg ugugcaagcg cacgcucgug      840

gaccggggcu gggggaacgg cugcgggcug uccggcaagg gcucccuggu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ugagcgugca cggcucccag cacagcggga ugaucggcua cgagacggac     1020

gaggaccggg ccaaggugga ggucaccccg aacuccccgc gcgccgaggc cacccugggc     1080

ggguucggca gccucgggcu ggacugcgag ccccggaccg gccuggacuu cuccgaccuc     1140

uacuaccuga cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccucccgu ggcacgcggg ggccgacacc ggcaccccgc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag cgccagaccg ucguggugcu gggcagccag     1320

gagggggcgg uccacacggc ccuggccggc gcccucgagg ccgagaugga cggggcgaag     1380

ggccggcugu ucuccgggca ccugaagugc cgccucaaga uggacaagcu gcggcugaag     1440

ggcgugagcu acucccucug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc cugcaagauc     1560

ccgguccaga uggcggugga caugcagacc cugacgcccg uggggcgccu caucaccgcc     1620

aaccccguca ucaccgagag caccgagaac uccaagauga ugcuggagcu ggacccgccg     1680

uucggcgaca gcuacaucgu gaucggggug ggcgacaaga agaucacgca ccacuggcac     1740

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc gaagcggaug     1800

gccgugcucg gggacaccgc cugggacuuc ggcagcgugg gcggcgucuu caacucccug     1860

gggaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1920

ugguucagcc agauccucau cgggacgcug cugguguggc ucggccugaa caccaagaac     1980

ggcuccauca gccugaccug ccucgcgcug ggcggcguga ugaucuuccu guccaccgcc     2040

gucagcgcc                                                             2049


<210>  1100
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) CDS GC

<400>  1100
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacc acgaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacgcuggug      840

gaccggggcu gggggaacgg cugcggccuc uccgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac cguggucgug     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cccugacgcc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu gcugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1101
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) CDS GC

<400>  1101
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucca ggacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1102
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) CDS GC

<400>  1102
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacacgugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac gagcaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac gcgcaagcug      360

cagacccgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccacgagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacg accaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acgcaguacg ucugcaagcg cacccuggug      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugca ggacacgggc     1020

cacgagaccg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac gggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac gguggucgug     1320

cugggcuccc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cgcuccacgg caccgugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cgcugacccc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcacggaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccgugcu gggggacacg gcgugggacu ucggcagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacgccaaga acggcuccau cagccugaug ugccucgcgc ugggcggcgu gcugaucuuc     2040

cucuccaccg ccgugagcgc c                                               2061


<210>  1103
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) CDS GC

<400>  1103
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacc acgaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacgcuggug      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugca ggacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac cguggucgug     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cccugacgcc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu gcugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1104
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS GC

<400>  1104
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cagcacccug gggaaggccu ucuccaccac gcucaagggc     1800

gcccagcgcc uggcggcccu gggggacacc gccugggacu ucggcagcau cgggggcgug     1860

uucaacucca ucggccgggc cguccaccag guguucgggg gcgccuuccg cacccucuuc     1920

gggggcauga gcuggaucac ccaggggcug augggcgcgc ugcuccugug gaugggggug     1980

aacgcccggg accgcuccau cgcccuggcc uuccucgcga cgggcggcgu ccugguguuc     2040

cuggccacca acgugcacgc c                                               2061


<210>  1105
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS GC

<400>  1105
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacacgugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac gagcaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac gcgcaagcug      360

cagacccgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccacgagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacg accaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acgcaguacg ucugcaagcg cacccuggug      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugaa cgacacgggc     1020

cacgagaccg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac gggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac gguggucgug     1320

cugggcuccc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cgcuccacgg caccgugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cgcugacccc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcacggaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accggagcgg cagcacccug gggaaggccu ucuccaccac gcucaagggc     1800

gcccagcgcc uggcggcccu gggggacacc gccugggacu ucggcagcau cgggggcgug     1860

uucaacucca ucggccgggc cguccaccag guguucgggg gcgccuuccg cacccucuuc     1920

gggggcauga gcuggaucac ccaggggcug augggcgcgc ugcuccugug gaugggggug     1980

aacgcccggg accgcuccau cgcccuggcc uuccucgcga cgggcggcgu ccugguguuc     2040

cuggccacca acgugcacgc c                                               2061


<210>  1106
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       CDS GC

<400>  1106
accagcaucg ggaucaucgg ccugcugcuc acgaccgcca uggcggccga gaucacccgc       60

cggggguccg ccuacuacau guaccuggac cgcagcgacg ccggcaaggc caucuccuuc      120

gcgaccacgc ugggcgugaa caagugccac gugcagauca uggaccucgg gcacaugugc      180

gacgccacca ugagcuacga gugcccgaug cuggacgagg gcgucgagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccacgugg guggucuacg ggaccugcca ccacaagaag      300

ggcgaggccc ggcgcagccg gcgcgccgug acccugcccu cccacagcac ccggaagcuc      360

cagacgcgcu cccagaccug gcuggagagc cgcgaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuuccggaa ccccggcuuc gcccucgucg cgguggccau cgccuggcug      480

cuggggucca gcaccuccca gaaggugauc uaccucguca ugauccugcu gaucgccccg      540

gccuacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

gggacguggg ucgacguggu gcucgagcac ggcggcugcg ucaccgugau ggcgcaggac      660

aagcccaccg uggacaucga gcuggucacc acgaccgugu ccaacauggc cgagguccgc      720

agcuacugcu acgaggccag caucuccgac auggccagcg acucccggug ccccacccag      780

ggggaggccu accuggacaa gcagagcgac acccaguacg ugugcaagcg cacgcucgug      840

gaccggggcu gggggaacgg cugcgggcug uucggcaagg gcucccuggu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ugagcgugca cggcucccag cacagcggga ugaucggcua cgagacggac     1020

gaggaccggg ccaaggugga ggucaccccg aacuccccgc gcgccgaggc cacccugggc     1080

ggguucggca gccucgggcu ggacugcgag ccccggaccg gccuggacuu cuccgaccuc     1140

uacuaccuga cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccucccgu ggcacgcggg ggccgacacc ggcaccccgc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag cgccagaccg ucguggugcu gggcagccag     1320

gagggggcgg uccacacggc ccuggccggc gcccucgagg ccgagaugga cggggcgaag     1380

ggccggcugu ucuccgggca ccugaagugc cgccucaaga uggacaagcu gcggcugaag     1440

ggcgugagcu acucccucug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc cugcaagauc     1560

ccgguccaga uggcggugga caugcagacc cugacgcccg uggggcgccu caucaccgcc     1620

aaccccguca ucaccgagag caccgagaac uccaagauga ugcuggagcu ggacccgccg     1680

uucggcgaca gcuacaucgu gaucggggug ggcgacaaga agaucacgca ccacuggcac     1740

cggagcggca gcacccuggg gaaggccuuc uccaccacgc ucaagggcgc ccagcgccug     1800

gcggcccugg gggacaccgc cugggacuuc ggcagcaucg ggggcguguu caacuccauc     1860

ggccgggccg uccaccaggu guucgggggc gccuuccgca cccucuucgg gggcaugagc     1920

uggaucaccc aggggcugau gggcgcgcug cuccugugga ugggggugaa cgcccgggac     1980

cgcuccaucg cccuggccuu ccucgcgacg ggcggcgucc ugguguuccu ggccaccaac     2040

gugcacgcc                                                             2049


<210>  1107
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS GC

<400>  1107
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacc acgaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacgcuggug      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac cguggucgug     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cccugacgcc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacg     1740

caccacuggc accggagcgg cagcacccug gggaaggccu ucuccaccac gcucaagggc     1800

gcccagcgcc uggcggcccu gggggacacc gccugggacu ucggcagcau cgggggcgug     1860

uucaacucca ucggccgggc cguccaccag guguucgggg gcgccuuccg cacccucuuc     1920

gggggcauga gcuggaucac ccaggggcug augggcgcgc ugcuccugug gaugggggug     1980

aacgcccggg accgcuccau cgcccuggcc uuccucgcga cgggcggcgu ccugguguuc     2040

cuggccacca acgugcacgc c                                               2061


<210>  1108
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS GC

<400>  1108
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaag                               2136


<210>  1109
<211>  2133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1109
aucaucaacg cccgcaagga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgacgugg ggugcuccgu cgacuucagc aag                                  2133


<210>  1110
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1110
aucaacgccc gcaaggagaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gacguggggu gcuccgucga cuucagcaag                                      2130


<210>  1111
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1111
aacgcccgca aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu ccgucgacuu cagcaag                                         2127


<210>  1112
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1112
gcccgcaagg agaagaagcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacgug     2100

gggugcuccg ucgacuucag caag                                            2124


<210>  1113
<211>  2121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1113
cgcaaggaga agaagcggcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcuccgucg acuucagcaa g                                               2121


<210>  1114
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1114
aaggagaaga agcggcgcgg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cguggggugc     2100

uccgucgacu ucagcaag                                                   2118


<210>  1115
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1115
gagaagaagc ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugcucc     2100

gucgacuuca gcaag                                                      2115


<210>  1116
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1116
aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcuccguc     2100

gacuucagca ag                                                         2112


<210>  1117
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1117
aagcggcgcg gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cuccgucgac     2100

uucagcaag                                                             2109


<210>  1118
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1118
cggcgcggcg ccgacaccag cguggggauc gugggccucc ugcugacgac cgccauggcg       60

gccgagguca cccggcgcgg cagcgccuac uacauguacc ucgaccggaa cgacgccggg      120

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      180

cuggggcaca ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg      300

ugccaccaca agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac      360

uccacccgca agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag      420

caccugaucc gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc      480

gccaucgccu ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc      540

cuccugaucg cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug      600

gaggggaugu ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc      660

gugauggccc aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac      720

auggccgagg uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc      780

cggugcccca cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc      840

aagcgcacgc uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc      900

cuggugaccu gcgccaaguu cgccugcucc aagaagauga ccgggaaguc cauccagccg      960

gagaaccugg aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc     1020

gucaacgaca ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac     1080

agcccccgcg cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc     1140

cggaccggcc uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg     1260

accccgcacu ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc     1320

cagaccgugg ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg     1380

cuggaggccg agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc     1440

cugaagaugg acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc     1500

accuucacca agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac     1560

gccgggaccg acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug     1620

acgcccgugg ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc     1680

aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg     1740

gagaagaaga ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc     1860

agcgugggcg gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug     1980

auguggcucg ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc     2040

ggcguccuga ucuuccucuc caccgcggug agcgccgacg uggggugcuc cgucgacuuc     2100

agcaag                                                                2106


<210>  1119
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1119
cgcggcgccg acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc       60

gaggucaccc ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag      120

gccaucagcu ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug      180

gggcacaugu gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc      300

caccacaaga agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc      360

acccgcaagc ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac      420

cugauccggg ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc      480

aucgccuggc uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc      540

cugaucgccc ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag      600

gggauguccg gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug      660

auggcccagg acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug      720

gccgaggucc gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg      780

ugccccaccc agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag      840

cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug      900

gugaccugcg ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag      960

aaccuggagu accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc     1020

aacgacaccg gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc     1080

ccccgcgcgg aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg     1140

accggccugg acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc     1260

ccgcacugga acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag     1320

accguggugg uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug     1380

gaggccgaga uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug     1440

aagauggaca agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg     1620

cccguggggc gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag     1680

augaugcucg agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc     1860

gugggcgggg cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug     1980

uggcucgggc ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc     2040

guccugaucu uccucuccac cgcggugagc gccgacgugg ggugcuccgu cgacuucagc     2100

aag                                                                   2103


<210>  1120
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1120
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag       60

gucacccggc gcggcagcgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc      120

aucagcuucc cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg      180

cacaugugcg acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac      300

cacaagaagg gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc      360

cgcaagcugc agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug      420

auccgggucg agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc      480

gccuggcucc uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug      540

aucgcccccg cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg      600

auguccggcg gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc      720

gagguccgca gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc      780

cccacccagg gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc      840

acgcuggucg accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug      900

accugcgcca aguucgccug cuccaagaag augaccggga aguccaucca gccggagaac      960

cuggaguacc gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac     1020

gacaccggcc acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc     1080

cgcgcggagg ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc     1140

ggccuggacu ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc     1320

gugguggucc ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag     1380

gccgagaugg acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg     1560

accgacggcc cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc     1620

guggggcgcc ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag     1740

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

guccgcggcg ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug     1860

ggcggggccc ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg     1980

cucgggcuga acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc     2040

cugaucuucc ucuccaccgc ggugagcgcc gacguggggu gcuccgucga cuucagcaag     2100


<210>  1121
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1121
gccgacacca gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc       60

acccggcgcg gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc      120

agcuucccga ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac      180

augugcgacg cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac      240

gacguggacu gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac      300

aagaaggggg aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc      360

aagcugcaga cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc      420

cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc      480

uggcuccugg ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc      540

gcccccgcgu acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug      600

uccggcggca cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc      660

caggacaagc ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag      720

guccgcagcu acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc      780

acccaggggg aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg      840

cuggucgacc ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc      900

ugcgccaagu ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug      960

gaguaccgca ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac     1020

accggccacg agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc     1080

gcggaggcca cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc     1140

cuggacuuca gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug     1320

gugguccugg gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc     1380

gagauggacg gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug     1440

gacaagcucc ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc     1500

aagauccccg cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggcccgu gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug     1620

gggcgccuga ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug     1680

cucgagcugg acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcca agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc     1860

ggggcccuca acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc     1980

gggcugaaca ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug     2040

aucuuccucu ccaccgcggu gagcgccgac guggggugcu ccgucgacuu cagcaag        2097


<210>  1122
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1122
gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc       60

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      360

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      660

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      840

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1020

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1140

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1320

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1560

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1620

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1740

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1800

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1860

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1920

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     1980

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2040

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caag           2094


<210>  1123
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1123
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc cgacgugggg ugcuccgucg acuucagcaa g              2091


<210>  1124
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1124
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccgc agucacccgg       60

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc cgacgugggg ugcuccgucg acuucagcaa g              2091


<210>  1125
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1125
cgcggcgccg acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc       60

gcagucaccc ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag      120

gccaucagcu ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug      180

gggcacaugu gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc      300

caccacaaga agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc      360

acccgcaagc ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac      420

cugauccggg ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc      480

aucgccuggc uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc      540

cugaucgccc ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag      600

gggauguccg gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug      660

auggcccagg acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug      720

gccgaggucc gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg      780

ugccccaccc agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag      840

cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug      900

gugaccugcg ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag      960

aaccuggagu accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc     1020

aacgacaccg gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc     1080

ccccgcgcgg aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg     1140

accggccugg acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc     1260

ccgcacugga acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag     1320

accguggugg uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug     1380

gaggccgaga uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug     1440

aagauggaca agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg     1620

cccguggggc gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag     1680

augaugcucg agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc     1860

gugggcgggg cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug     1980

uggcucgggc ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc     2040

guccugaucu uccucuccac cgcggugagc gccgacgugg ggugcuccgu cgacuucagc     2100

aag                                                                   2103


<210>  1126
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1126
cggggccuga gcucccgcaa gcggcgcagc cacgacgugc ucaccgucca guuccugauc       60

cuggggaugc uccugaugac cggcgggguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu ccgucgacuu cagcaag                                         2127


<210>  1127
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1127
cggggccuga gcucccgcaa gcggcgcagc cacgacgugc ucaccgucca guuccugauc       60

cuggggaugc uccugaugac cggcgcaguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu ccgucgacuu cagcaag                                         2127


<210>  1128
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1128
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugc                                                   2118


<210>  1129
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1129
aucaucaacg cccgcaagga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgacgugg ggugc                                                      2115


<210>  1130
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1130
aucaacgccc gcaaggagaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gacguggggu gc                                                         2112


<210>  1131
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1131
aacgcccgca aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugc                                                             2109


<210>  1132
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1132
gcccgcaagg agaagaagcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacgug     2100

gggugc                                                                2106


<210>  1133
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1133
cgcaaggaga agaagcggcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugc                                                                   2103


<210>  1134
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1134
aaggagaaga agcggcgcgg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cguggggugc     2100


<210>  1135
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1135
gagaagaagc ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugc        2097


<210>  1136
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1136
aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugc           2094


<210>  1137
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1137
aagcggcgcg gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug c              2091


<210>  1138
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1138
cggcgcggcg ccgacaccag cguggggauc gugggccucc ugcugacgac cgccauggcg       60

gccgagguca cccggcgcgg cagcgccuac uacauguacc ucgaccggaa cgacgccggg      120

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      180

cuggggcaca ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg      300

ugccaccaca agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac      360

uccacccgca agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag      420

caccugaucc gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc      480

gccaucgccu ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc      540

cuccugaucg cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug      600

gaggggaugu ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc      660

gugauggccc aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac      720

auggccgagg uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc      780

cggugcccca cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc      840

aagcgcacgc uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc      900

cuggugaccu gcgccaaguu cgccugcucc aagaagauga ccgggaaguc cauccagccg      960

gagaaccugg aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc     1020

gucaacgaca ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac     1080

agcccccgcg cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc     1140

cggaccggcc uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg     1260

accccgcacu ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc     1320

cagaccgugg ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg     1380

cuggaggccg agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc     1440

cugaagaugg acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc     1500

accuucacca agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac     1560

gccgggaccg acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug     1620

acgcccgugg ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc     1680

aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg     1740

gagaagaaga ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc     1860

agcgugggcg gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug     1980

auguggcucg ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc     2040

ggcguccuga ucuuccucuc caccgcggug agcgccgacg uggggugc                  2088


<210>  1139
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1139
cgcggcgccg acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc       60

gaggucaccc ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag      120

gccaucagcu ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug      180

gggcacaugu gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc      300

caccacaaga agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc      360

acccgcaagc ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac      420

cugauccggg ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc      480

aucgccuggc uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc      540

cugaucgccc ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag      600

gggauguccg gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug      660

auggcccagg acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug      720

gccgaggucc gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg      780

ugccccaccc agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag      840

cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug      900

gugaccugcg ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag      960

aaccuggagu accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc     1020

aacgacaccg gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc     1080

ccccgcgcgg aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg     1140

accggccugg acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc     1260

ccgcacugga acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag     1320

accguggugg uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug     1380

gaggccgaga uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug     1440

aagauggaca agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg     1620

cccguggggc gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag     1680

augaugcucg agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc     1860

gugggcgggg cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug     1980

uggcucgggc ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc     2040

guccugaucu uccucuccac cgcggugagc gccgacgugg ggugc                     2085


<210>  1140
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1140
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag       60

gucacccggc gcggcagcgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc      120

aucagcuucc cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg      180

cacaugugcg acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac      300

cacaagaagg gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc      360

cgcaagcugc agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug      420

auccgggucg agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc      480

gccuggcucc uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug      540

aucgcccccg cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg      600

auguccggcg gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc      720

gagguccgca gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc      780

cccacccagg gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc      840

acgcuggucg accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug      900

accugcgcca aguucgccug cuccaagaag augaccggga aguccaucca gccggagaac      960

cuggaguacc gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac     1020

gacaccggcc acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc     1080

cgcgcggagg ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc     1140

ggccuggacu ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc     1320

gugguggucc ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag     1380

gccgagaugg acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg     1560

accgacggcc cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc     1620

guggggcgcc ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag     1740

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

guccgcggcg ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug     1860

ggcggggccc ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg     1980

cucgggcuga acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc     2040

cugaucuucc ucuccaccgc ggugagcgcc gacguggggu gc                        2082


<210>  1141
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1141
gccgacacca gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc       60

acccggcgcg gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc      120

agcuucccga ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac      180

augugcgacg cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac      240

gacguggacu gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac      300

aagaaggggg aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc      360

aagcugcaga cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc      420

cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc      480

uggcuccugg ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc      540

gcccccgcgu acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug      600

uccggcggca cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc      660

caggacaagc ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag      720

guccgcagcu acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc      780

acccaggggg aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg      840

cuggucgacc ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc      900

ugcgccaagu ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug      960

gaguaccgca ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac     1020

accggccacg agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc     1080

gcggaggcca cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc     1140

cuggacuuca gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug     1320

gugguccugg gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc     1380

gagauggacg gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug     1440

gacaagcucc ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc     1500

aagauccccg cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggcccgu gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug     1620

gggcgccuga ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug     1680

cucgagcugg acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcca agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc     1860

ggggcccuca acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc     1980

gggcugaaca ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug     2040

aucuuccucu ccaccgcggu gagcgccgac guggggugc                            2079


<210>  1142
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1142
gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc       60

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      360

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      660

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      840

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1020

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1140

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1320

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1560

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1620

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1740

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1800

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1860

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1920

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     1980

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2040

uuccucucca ccgcggugag cgccgacgug gggugc                               2076


<210>  1143
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1143
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc cgacgugggg ugc                                  2073


<210>  1144
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1144
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccgc agucacccgg       60

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc cgacgugggg ugc                                  2073


<210>  1145
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1145
cgcggcgccg acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc       60

gcagucaccc ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag      120

gccaucagcu ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug      180

gggcacaugu gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc      300

caccacaaga agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc      360

acccgcaagc ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac      420

cugauccggg ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc      480

aucgccuggc uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc      540

cugaucgccc ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag      600

gggauguccg gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug      660

auggcccagg acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug      720

gccgaggucc gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg      780

ugccccaccc agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag      840

cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug      900

gugaccugcg ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag      960

aaccuggagu accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc     1020

aacgacaccg gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc     1080

ccccgcgcgg aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg     1140

accggccugg acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc     1260

ccgcacugga acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag     1320

accguggugg uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug     1380

gaggccgaga uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug     1440

aagauggaca agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg     1620

cccguggggc gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag     1680

augaugcucg agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc     1860

gugggcgggg cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug     1980

uggcucgggc ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc     2040

guccugaucu uccucuccac cgcggugagc gccgacgugg ggugc                     2085


<210>  1146
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1146
cggggccuga gcucccgcaa gcggcgcagc cacgacgugc ucaccgucca guuccugauc       60

cuggggaugc uccugaugac cggcgggguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugc                                                             2109


<210>  1147
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1147
cggggccuga gcucccgcaa gcggcgcagc cacgacgugc ucaccgucca guuccugauc       60

cuggggaugc uccugaugac cggcgcaguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugc                                                             2109


<210>  1148
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1148
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgac                                                             2109


<210>  1149
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1149
aucaucaacg cccgcaagga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgac                                                                2106


<210>  1150
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1150
aucaacgccc gcaaggagaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gac                                                                   2103


<210>  1151
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1151
aacgcccgca aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100


<210>  1152
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1152
gcccgcaagg agaagaagcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgac        2097


<210>  1153
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1153
cgcaaggaga agaagcggcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgac           2094


<210>  1154
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1154
aaggagaaga agcggcgcgg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga c              2091


<210>  1155
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1155
gagaagaagc ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgac                  2088


<210>  1156
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1156
aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgac                     2085


<210>  1157
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1157
aagcggcgcg gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg ac                        2082


<210>  1158
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1158
cggcgcggcg ccgacaccag cguggggauc gugggccucc ugcugacgac cgccauggcg       60

gccgagguca cccggcgcgg cagcgccuac uacauguacc ucgaccggaa cgacgccggg      120

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      180

cuggggcaca ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg      300

ugccaccaca agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac      360

uccacccgca agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag      420

caccugaucc gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc      480

gccaucgccu ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc      540

cuccugaucg cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug      600

gaggggaugu ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc      660

gugauggccc aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac      720

auggccgagg uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc      780

cggugcccca cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc      840

aagcgcacgc uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc      900

cuggugaccu gcgccaaguu cgccugcucc aagaagauga ccgggaaguc cauccagccg      960

gagaaccugg aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc     1020

gucaacgaca ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac     1080

agcccccgcg cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc     1140

cggaccggcc uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg     1260

accccgcacu ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc     1320

cagaccgugg ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg     1380

cuggaggccg agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc     1440

cugaagaugg acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc     1500

accuucacca agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac     1560

gccgggaccg acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug     1620

acgcccgugg ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc     1680

aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg     1740

gagaagaaga ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc     1860

agcgugggcg gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug     1980

auguggcucg ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc     2040

ggcguccuga ucuuccucuc caccgcggug agcgccgac                            2079


<210>  1159
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1159
cgcggcgccg acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc       60

gaggucaccc ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag      120

gccaucagcu ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug      180

gggcacaugu gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc      300

caccacaaga agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc      360

acccgcaagc ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac      420

cugauccggg ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc      480

aucgccuggc uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc      540

cugaucgccc ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag      600

gggauguccg gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug      660

auggcccagg acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug      720

gccgaggucc gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg      780

ugccccaccc agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag      840

cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug      900

gugaccugcg ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag      960

aaccuggagu accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc     1020

aacgacaccg gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc     1080

ccccgcgcgg aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg     1140

accggccugg acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc     1260

ccgcacugga acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag     1320

accguggugg uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug     1380

gaggccgaga uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug     1440

aagauggaca agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg     1620

cccguggggc gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag     1680

augaugcucg agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc     1860

gugggcgggg cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug     1980

uggcucgggc ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc     2040

guccugaucu uccucuccac cgcggugagc gccgac                               2076


<210>  1160
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1160
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag       60

gucacccggc gcggcagcgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc      120

aucagcuucc cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg      180

cacaugugcg acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac      300

cacaagaagg gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc      360

cgcaagcugc agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug      420

auccgggucg agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc      480

gccuggcucc uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug      540

aucgcccccg cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg      600

auguccggcg gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc      720

gagguccgca gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc      780

cccacccagg gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc      840

acgcuggucg accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug      900

accugcgcca aguucgccug cuccaagaag augaccggga aguccaucca gccggagaac      960

cuggaguacc gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac     1020

gacaccggcc acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc     1080

cgcgcggagg ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc     1140

ggccuggacu ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc     1320

gugguggucc ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag     1380

gccgagaugg acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg     1560

accgacggcc cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc     1620

guggggcgcc ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag     1740

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

guccgcggcg ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug     1860

ggcggggccc ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg     1980

cucgggcuga acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc     2040

cugaucuucc ucuccaccgc ggugagcgcc gac                                  2073


<210>  1161
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1161
gccgacacca gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc       60

acccggcgcg gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc      120

agcuucccga ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac      180

augugcgacg cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac      240

gacguggacu gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac      300

aagaaggggg aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc      360

aagcugcaga cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc      420

cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc      480

uggcuccugg ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc      540

gcccccgcgu acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug      600

uccggcggca cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc      660

caggacaagc ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag      720

guccgcagcu acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc      780

acccaggggg aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg      840

cuggucgacc ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc      900

ugcgccaagu ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug      960

gaguaccgca ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac     1020

accggccacg agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc     1080

gcggaggcca cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc     1140

cuggacuuca gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug     1320

gugguccugg gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc     1380

gagauggacg gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug     1440

gacaagcucc ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc     1500

aagauccccg cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggcccgu gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug     1620

gggcgccuga ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug     1680

cucgagcugg acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcca agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc     1860

ggggcccuca acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc     1980

gggcugaaca ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug     2040

aucuuccucu ccaccgcggu gagcgccgac                                      2070


<210>  1162
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1162
gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc       60

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      360

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      660

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      840

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1020

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1140

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1320

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1560

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1620

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1740

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1800

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1860

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1920

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     1980

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2040

uuccucucca ccgcggugag cgccgac                                         2067


<210>  1163
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1163
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc cgac                                            2064


<210>  1164
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1164
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccgc agucacccgg       60

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc cgac                                            2064


<210>  1165
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1165
cgcggcgccg acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc       60

gcagucaccc ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag      120

gccaucagcu ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug      180

gggcacaugu gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc      300

caccacaaga agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc      360

acccgcaagc ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac      420

cugauccggg ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc      480

aucgccuggc uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc      540

cugaucgccc ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag      600

gggauguccg gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug      660

auggcccagg acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug      720

gccgaggucc gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg      780

ugccccaccc agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag      840

cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug      900

gugaccugcg ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag      960

aaccuggagu accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc     1020

aacgacaccg gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc     1080

ccccgcgcgg aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg     1140

accggccugg acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc     1260

ccgcacugga acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag     1320

accguggugg uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug     1380

gaggccgaga uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug     1440

aagauggaca agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg     1620

cccguggggc gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag     1680

augaugcucg agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc     1860

gugggcgggg cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug     1980

uggcucgggc ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc     2040

guccugaucu uccucuccac cgcggugagc gccgac                               2076


<210>  1166
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1166
cggggccuga gcucccgcaa gcggcgcagc cacgacgugc ucaccgucca guuccugauc       60

cuggggaugc uccugaugac cggcgggguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100


<210>  1167
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1167
cggggccuga gcucccgcaa gcggcgcagc cacgacgugc ucaccgucca guuccugauc       60

cuggggaugc uccugaugac cggcgcaguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100


<210>  1168
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1168
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgcc                                                                2106


<210>  1169
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1169
aucaacgccc gcaaggagaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100


<210>  1170
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1170
aucaucaacg cccgcaagga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gcc                                                                   2103


<210>  1171
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1171
aacgcccgca aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgcc        2097


<210>  1172
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1172
gcccgcaagg agaagaagcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgcc           2094


<210>  1173
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1173
cgcaaggaga agaagcggcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc c              2091


<210>  1174
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1174
aaggagaaga agcggcgcgg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgcc                  2088


<210>  1175
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1175
gagaagaagc ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgcc                     2085


<210>  1176
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1176
aagaagcggc gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg cc                        2082


<210>  1177
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1177
aagcggcgcg gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgcc                            2079


<210>  1178
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1178
cggcgcggcg ccgacaccag cguggggauc gugggccucc ugcugacgac cgccauggcg       60

gccgagguca cccggcgcgg cagcgccuac uacauguacc ucgaccggaa cgacgccggg      120

gaggccauca gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac      180

cuggggcaca ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg      300

ugccaccaca agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac      360

uccacccgca agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag      420

caccugaucc gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc      480

gccaucgccu ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc      540

cuccugaucg cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug      600

gaggggaugu ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc      660

gugauggccc aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac      720

auggccgagg uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc      780

cggugcccca cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc      840

aagcgcacgc uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc      900

cuggugaccu gcgccaaguu cgccugcucc aagaagauga ccgggaaguc cauccagccg      960

gagaaccugg aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc     1020

gucaacgaca ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac     1080

agcccccgcg cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc     1140

cggaccggcc uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg     1260

accccgcacu ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc     1320

cagaccgugg ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg     1380

cuggaggccg agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc     1440

cugaagaugg acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc     1500

accuucacca agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac     1560

gccgggaccg acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug     1620

acgcccgugg ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc     1680

aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg     1740

gagaagaaga ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc     1860

agcgugggcg gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug     1980

auguggcucg ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc     2040

ggcguccuga ucuuccucuc caccgcggug agcgcc                               2076


<210>  1179
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1179
cgcggcgccg acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc       60

gaggucaccc ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag      120

gccaucagcu ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug      180

gggcacaugu gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc      300

caccacaaga agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc      360

acccgcaagc ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac      420

cugauccggg ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc      480

aucgccuggc uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc      540

cugaucgccc ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag      600

gggauguccg gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug      660

auggcccagg acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug      720

gccgaggucc gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg      780

ugccccaccc agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag      840

cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug      900

gugaccugcg ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag      960

aaccuggagu accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc     1020

aacgacaccg gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc     1080

ccccgcgcgg aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg     1140

accggccugg acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc     1260

ccgcacugga acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag     1320

accguggugg uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug     1380

gaggccgaga uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug     1440

aagauggaca agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg     1620

cccguggggc gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag     1680

augaugcucg agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc     1860

gugggcgggg cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug     1980

uggcucgggc ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc     2040

guccugaucu uccucuccac cgcggugagc gcc                                  2073


<210>  1180
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1180
ggcgccgaca ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag       60

gucacccggc gcggcagcgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc      120

aucagcuucc cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg      180

cacaugugcg acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac      300

cacaagaagg gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc      360

cgcaagcugc agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug      420

auccgggucg agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc      480

gccuggcucc uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug      540

aucgcccccg cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg      600

auguccggcg gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc      720

gagguccgca gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc      780

cccacccagg gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc      840

acgcuggucg accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug      900

accugcgcca aguucgccug cuccaagaag augaccggga aguccaucca gccggagaac      960

cuggaguacc gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac     1020

gacaccggcc acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc     1080

cgcgcggagg ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc     1140

ggccuggacu ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc     1320

gugguggucc ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag     1380

gccgagaugg acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg     1560

accgacggcc cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc     1620

guggggcgcc ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag     1740

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

guccgcggcg ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug     1860

ggcggggccc ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg     1980

cucgggcuga acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc     2040

cugaucuucc ucuccaccgc ggugagcgcc                                      2070


<210>  1181
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1181
gccgacacca gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc       60

acccggcgcg gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc      120

agcuucccga ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac      180

augugcgacg cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac      240

gacguggacu gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac      300

aagaaggggg aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc      360

aagcugcaga cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc      420

cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc      480

uggcuccugg ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc      540

gcccccgcgu acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug      600

uccggcggca cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc      660

caggacaagc ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag      720

guccgcagcu acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc      780

acccaggggg aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg      840

cuggucgacc ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc      900

ugcgccaagu ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug      960

gaguaccgca ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac     1020

accggccacg agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc     1080

gcggaggcca cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc     1140

cuggacuuca gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug     1320

gugguccugg gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc     1380

gagauggacg gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug     1440

gacaagcucc ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc     1500

aagauccccg cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggcccgu gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug     1620

gggcgccuga ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug     1680

cucgagcugg acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcca agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc     1860

ggggcccuca acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc     1980

gggcugaaca ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug     2040

aucuuccucu ccaccgcggu gagcgcc                                         2067


<210>  1182
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1182
gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc       60

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      360

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      660

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      840

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1020

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1140

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1320

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1560

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1620

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1740

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1800

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1860

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1920

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     1980

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2040

uuccucucca ccgcggugag cgcc                                            2064


<210>  1183
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS GC

<400>  1183
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1184
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1184
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccgc agucacccgg       60

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1185
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1185
cgcggcgccg acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc       60

gcagucaccc ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag      120

gccaucagcu ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug      180

gggcacaugu gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc      300

caccacaaga agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc      360

acccgcaagc ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac      420

cugauccggg ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc      480

aucgccuggc uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc      540

cugaucgccc ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag      600

gggauguccg gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug      660

auggcccagg acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug      720

gccgaggucc gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg      780

ugccccaccc agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag      840

cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug      900

gugaccugcg ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag      960

aaccuggagu accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc     1020

aacgacaccg gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc     1080

ccccgcgcgg aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg     1140

accggccugg acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc     1260

ccgcacugga acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag     1320

accguggugg uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug     1380

gaggccgaga uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug     1440

aagauggaca agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg     1620

cccguggggc gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag     1680

augaugcucg agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc     1860

gugggcgggg cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug     1980

uggcucgggc ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc     2040

guccugaucu uccucuccac cgcggugagc gcc                                  2073


<210>  1186
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1186
cggggccuga gcucccgcaa gcggcgcagc cacgacgugc ucaccgucca guuccugauc       60

cuggggaugc uccugaugac cggcgggguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgcc        2097


<210>  1187
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS GC

<400>  1187
cggggccuga gcucccgcaa gcggcgcagc cacgacgugc ucaccgucca guuccugauc       60

cuggggaugc uccugaugac cggcgcaguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgcc        2097


<210>  1188
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long CDS GC

<400>  1188
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cuccgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaag                               2136


<210>  1189
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long CDS GC

<400>  1189
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cuccgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca cgugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accacgagca ccugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacgcgca agcugcagac ccggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacg      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac cuggguggac      660

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      720

aucgagcucg ugacgaccac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacacgca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca cgggccacga gaccgacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggacgggcc uggacuucag cgaccuguac     1200

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg cucccaggag     1380

ggggccgugc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacgcuc     1560

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1620

gcccagaugg ccguggacau gcagacgcug acccccgucg ggcgccugau caccgcgaac     1680

cccgugauca ccgagagcac ggagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacccacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1860

gugcuggggg acacggcgug ggacuucggc agcgucggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacccugcug auguggcucg ggcugaacgc caagaacggc     2040

uccaucagcc ugaugugccu cgcgcugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1190
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long CDS GC

<400>  1190
cggaucauca acgcccgcaa ggagcggaag cgccggggcg ccgacaccag caucgggauc       60

aucggccugc ugcucacgac cgccauggcg gccgagauca cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc aaggccaucu ccuucgcgac cacgcugggc      180

gugaacaagu gccacgugca gaucauggac cucgggcaca ugugcgacgc caccaugagc      240

uacgagugcc cgaugcugga cgagggcguc gagcccgacg acguggacug cuggugcaac      300

accaccucca cguggguggu cuacgggacc ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgugacccu gcccucccac agcacccgga agcuccagac gcgcucccag      420

accuggcugg agagccgcga guacaccaag caccugauca agguggagaa cuggaucuuc      480

cggaaccccg gcuucgcccu cgucgcggug gccaucgccu ggcugcuggg guccagcacc      540

ucccagaagg ugaucuaccu cgucaugauc cugcugaucg ccccggccua cagcauccgc      600

ugcaucggcg uguccaaccg ggacuucgug gaggggauga gcggcgggac gugggucgac      660

guggugcucg agcacggcgg cugcgucacc gugauggcgc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccagcaucu ccgacauggc cagcgacucc cggugcccca cccaggggga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagcgcacgc ucguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caagggcucc cuggucacgu gcgcgaaguu cacgugcagc      960

aagaagauga ccgggaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc ggcuacgaga cggacgagga ccgggccaag     1080

guggagguca ccccgaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccuc     1140

gggcuggacu gcgagccccg gaccggccug gacuucuccg accucuacua ccugacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu cccguggcac     1260

gcgggggccg acaccggcac cccgcacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagcgcca gaccgucgug gugcugggca gccaggaggg ggcgguccac     1380

acggcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggccg gcuguucucc     1440

gggcaccuga agugccgccu caagauggac aagcugcggc ugaagggcgu gagcuacucc     1500

cucugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggccccugca agaucccggu ccagauggcg     1620

guggacaugc agacccugac gcccgugggg cgccucauca ccgccaaccc cgucaucacc     1680

gagagcaccg agaacuccaa gaugaugcug gagcuggacc cgccguucgg cgacagcuac     1740

aucgugaucg gggugggcga caagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgcgaagc ggauggccgu gcucggggac     1860

accgccuggg acuucggcag cgugggcggc gucuucaacu cccuggggaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggga cgcugcuggu guggcucggc cugaacacca agaacggcuc caucagccug     2040

accugccucg cgcugggcgg cgugaugauc uuccugucca ccgccgucag cgccgacgug     2100

gggugcuccg uggacuucag caag                                            2124


<210>  1191
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long CDS GC

<400>  1191
cggaucauca acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cuccgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      720

aucgagcucg ugaccacgac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

guggaguuca aggacgcgca cgccaagcgc cagaccgugg ucgugcuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgucg ggcgccugau caccgcgaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgucggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgcggug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1192
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long CDS GC

<400>  1192
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggugacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu gguggccgug gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ucaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucguc gagggcauga gcggcgggac cuggguggac      660

gugguccugg agcacggcgg gugcgugacg gugauggccc aggacaagcc gaccgucgac      720

aucgagcugg ugaccaccac gguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgucacgu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc gucaacgaca ccggcuacga gaccgacgag     1080

aaccgcgcca agguggaggu gacccccaac uccccgcggg ccgaggcgac ccucgggggc     1140

uucgggagcc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggggc cgacaccggc accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgugg ucgugcucgg gagccaggag     1380

ggcgccgugc acacggcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

aagcuguucu ccgggcaccu caagugccgc cugaagaugg acaagcuccg gcugaagggc     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca aggugcccgc ggagacccuc     1560

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caagaucccc     1620

gugcagaugg ccgucgacau gcagacccug acgccggugg ggcgccugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gacaagaaga ucacgcacca cuggcaccgg     1800

uccgggagca ccaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcguguucaa cucccucggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacgcugcuc guguggcugg gccugaacac caagaacggg     2040

uccaucagcc ugaccugccu cgcccugggc ggcgugauga ucuuccucuc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1193
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long CDS GC

<400>  1193
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

gugggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gucaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggucacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca agguggagaa cuggaucuuc      480

cggaaccccg gguucacccu gguggccguc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggugaugauc cugcucaucg cccccgcgua cagcauccgc      600

ugcaucgggg ucuccaaccg ggacuucgug gagggcauga gcggcgggac guggguggac      660

gucgugcugg agcacggcgg gugcgugacc gucauggccc aggacaagcc gaccguggac      720

aucgagcugg ugaccacgac cgucuccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cagcgacucc cggugcccca cccagggcga ggcguaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgacg agaaccgcgc caagguggag     1080

gucaccccca acuccccgcg ggccgaggcc acccucggcg gguucggcag ccuggggcug     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccugu acuaccugac caugaacaac     1200

aagcacuggc ucgugcacaa ggagugguuc cacgacaucc cccugcccug gcacgcgggc     1260

gccgacacgg ggaccccgca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaagc ggcagaccgu cguggugcuc ggcagccagg agggggcggu ccacaccgcc     1380

cuggccggcg cccuggaggc ggagauggac ggggccaagg gccgccucuu cuccggccac     1440

cugaagugcc ggcugaagau ggacaagcuc cgccugaagg gggugagcua cucccugugc     1500

acggccgccu ucaccuucac caaggugccc gccgagaccc uccacggcac cgucacggug     1560

gaggugcagu acgcggggac cgacggcccc ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugaccccggu gggccggcug aucacggcca accccgucau caccgagagc     1680

accgagaacu ccaagaugau gcucgagcug gacccgcccu ucggggacag cuacaucgug     1740

aucggcgugg gggacaagaa gaucacccac cacuggcacc gcuccggcag cacgaucggg     1800

aaggccuucg aggcgaccgu ccggggcgcc aagcgcaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg ggagcguggg cggggucuuc aacucccucg gcaaggggau ccaccagauc     1920

uucggcgccg cguucaagag ccuguucggc gggauguccu gguucagcca gauccugauc     1980

ggcacccugc ucguguggcu ggggcugaac acgaagaacg gcuccaucag ccugaccugc     2040

cuggcccucg ggggcgugau gaucuuccuc uccaccgccg ucagcgccga cgugggcugc     2100

uccguggacu ucagcaag                                                   2118


<210>  1194
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long CDS GC

<400>  1194
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccgca agaagggcga ggcccggcgc      360

agccggcgcg cggugacccu ccccucccac agcacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gguucgcccu gguggccgug gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ucaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgg      600

ugcaucgggg uguccaaccg cgacuucguc gagggcauga gcggcgggac cuggguggac      660

gugguccugg agcacggcgg gugcgugacg gugauggccc aggacaagcc gaccgucgac      720

aucgagcugg ugaccaccac gguguccaac auggccgagg uccggagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcggaccc ugguggaccg cggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgucacgu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc gucaacgaca ccggcuacga gaccgacgag     1080

aaccgggcca agguggaggu gacccccaac uccccgcgcg ccgaggcgac ccucgggggc     1140

uucgggagcc ugggccugga cugcgagccc cggaccggcc ucgacuucuc cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggggc cgacaccggc accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcucgg gagccaggag     1380

ggcgccgugc acacggcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

aagcuguucu ccgggcaccu caagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca aggugcccgc ggagacccuc     1560

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caagaucccc     1620

gugcagaugg ccgucgacau gcagacccug acgccggugg ggcggcugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gacaagaaga ucacgcacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gcgaccgugc ggggcgccaa gcgcauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcguguucaa cucccucggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacgcugcuc guguggcugg gccugaacac caagaacggg     2040

uccaucagcc ugaccugccu cgcccugggc ggcgugauga ucuuccucuc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1195
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long CDS GC

<400>  1195
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggugacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu gguggccgug gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ucaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucguc gagggcauga gcggcgggac cuggguggac      660

gugguccugg agcacggcgg gugcgugacg gugauggccc aggacaagcc gaccgucgac      720

aucgagcugg ugaccaccac gguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgucacgu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugacc gucaacgaca ucggcuacga gaccgacgag     1080

aaccgcgcca agguggaggu gacccccaac uccccgcggg ccgaggcgac ccucgggggc     1140

uucgggagcc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggggc cgacaccggc accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgugg ucgugcucgg gagccaggag     1380

ggcgccgugc acacggcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

aagcuguucu ccgggcaccu caagugccgc cugaagaugg acaagcuccg gcugaagggc     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca aggugcccgc ggagacccuc     1560

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caagaucccc     1620

gugcagaugg ccgucgacau gcagacccug acgccggugg ggcgccugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gacaagaaga ucacgcacca cuggcaccgg     1800

uccgggagca ccaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcguguucaa cucccucggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacgcugcuc guguggcugg gccugaacac caagaacggg     2040

uccaucagcc ugaccugccu cgcccugggc ggcgugauga ucuuccucuc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1196
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long CDS GC

<400>  1196
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggugacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu gguggccgug gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ucaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucguc gagggcauga gcggcgggac cuggguggac      660

gugguccugg agcacggcgg gugcgugacg gugauggccc aggacaagcc gaccgucgac      720

aucgagcugg ugaccaccac gguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgucacgu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc ggcuacgaga ccgacgagaa ccgcgccaag     1080

gucgagguga cccccaacuc cccgcgggcc gaggcgaccc ucgggggcuu cgggagccug     1140

ggccuggacu gcgagccccg caccggccuc gacuucuccg accuguacua ccugaccaug     1200

aacaacaagc acuggcucgu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggggccg acacgggcac cccgcacugg aacaacaagg aggcccuggu cgaguucaag     1320

gacgcccacg cgaagcggca gaccguggug guccucggga gccaggaggg cgccgugcac     1380

accgcccugg ccggggcgcu ggaggccgag auggacggcg ccaagggcaa gcuguucucc     1440

gggcaccuca agugccgccu gaagauggac aagcuccggc ugaagggcgu gagcuacucc     1500

cugugcacgg ccgccuucac cuucaccaag guccccgcgg agacccucca cgggaccgug     1560

acgguggagg uccaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1620

guggacaugc agacccugac cccggucggg cgccugauca cggccaaccc cgugaucacc     1680

gagagcaccg agaacuccaa gaugaugcuc gagcuggacc cgcccuucgg cgacagcuac     1740

aucgugaucg gggucggcga caagaagauc acccaccacu ggcaccgguc cgggagcacg     1800

aucggcaagg ccuucgaggc gaccgugcgc ggcgccaagc ggauggccgu gcugggggac     1860

accgccuggg acuucggcag cgucgggggc guguucaacu cccucgggaa gggcauccac     1920

cagaucuucg gcgccgcguu caagagccug uucgggggca uguccugguu cagccagauc     1980

cugaucggga cccugcucgu guggcugggc cugaacacga agaacggguc caucagccug     2040

accugccucg cccugggcgg cgucaugauc uuccucucca ccgccgugag cgccgacgug     2100

gggugcuccg ucgacuucag caag                                            2124


<210>  1197
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long CDS GC

<400>  1197
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

cggggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1198
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long CDS GC

<400>  1198
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacgcgag cguggggauc       60

gucggccugc uccugaccac cgccauggcc gccgagguga cgcgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgcgggc gaggccauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

acgaccagca ccuggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg cggucacgcu ccccagccac uccacccgga agcugcagac ccgcagccag      420

accuggcugg agucccggga guacacgaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggccgccgcc gcgaucgccu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc cacgguggac      720

aucgagcugg ucaccaccac cgugagcaac auggcggagg ugcgcuccua cugcuacgag      780

gccagcaucu ccgacauggc cagcgacagc cggugcccca cgcagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugaccu gcgccaaguu cgcgugcucc      960

aagaagauga cgggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau caccccgaac agcccccggg ccgaggccac gcucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc ggacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgcggucc acaccgcccu ggccggggcc cuggaggccg agauggacgg cgcgaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgccgccuuc acguucacca agauccccgc cgagacccuc     1560

cacgggaccg ugaccgucga ggugcaguac gccggcacgg acggccccug caaggugccg     1620

gcgcagaugg ccgucgacau gcagacccug acccccgugg ggcggcugau caccgccaac     1680

cccgugauca cggagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccacggugc ggggcgcgaa gcgcauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgcggcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac caagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc cacggccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1199
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long CDS GC

<400>  1199
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg ggcuggcgcu ggccgccgcc gccaucgcgu ggcuccuggg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cuccugaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcucg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccug      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cuggugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccucga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcuc     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcuggg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cucgaggccg agauggacgg cgccaagggc     1440

cgccugagcu ccgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc     1500

gugagcuacu cccucugcac cgcggccuuc accuucacca agauccccgc cgagacccug     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcucau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccucgggg acaccgccug ggacuucggc agcgucgggg gcgcccugaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg gacccugcug auguggcucg gccugaacac gaagaacggg     2040

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1200
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long CDS GC

<400>  1200
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cugguaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1201
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long CDS GC

<400>  1201
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgaga uccgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga agguggagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgugg ucgugcucgg gucccaggag     1380

ggcgccgugc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggugga gguccaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cgguggacau gcagacccug acccccgucg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1202
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long CDS GC

<400>  1202
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugcug     1620

gcccagaugg cggucgacau gcagacccug accccggugg ggcggcucau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccucgggg acaccgccug ggacuucggc agcgucgggg gcgcccugaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg gacccugcug auguggcucg gccugaacac gaagaacggg     2040

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1203
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long CDS GC

<400>  1203
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgacccugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca cguggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac gcgcagccag      420

accuggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac gugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gacggacgag     1080

aaccgcgcga aggucgagau caccccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acgcccgugg ggcggcugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacgcacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacgcuccug auguggcugg gccucaacac caagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1204
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long CDS GC

<400>  1204
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccguggug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaag                               2136


<210>  1205
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long CDS GC

<400>  1205
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagcg ccuggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg cggucacgcu ccccagccac uccacccgga agcugcagac ccgcccccag      420

accuggcugg agagccggga guacacgaag caccucaucc gcguggagaa cuggaucuuc      480

cggaacccgg gguucgcccu ggccgccgcc gcgaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg cccccgccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc cacgguggac      720

aucgagcugg ucaccaccac cguguccaac auggcggagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cgcagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cgcgugcagc      960

aagaagauga cgggcaaguc cauccagccg gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac agcccccggg ccgaggccac gcucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggggc ggacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgcggucc acaccgcccu ggccggggcc cuggaggccg agauggacgg cgcgaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgccgccuuc acguucacca agauccccgc cgagacccuc     1560

cacgggaccg ugaccgucga ggugcaguac gccggcacgg acggcccgug caaggugccc     1620

gcgcagaugg ccgucgacau gcagacccug acccccgugg ggcggcugau caccgccaac     1680

cccgugauca cggaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggucca ccaucggcaa ggccuucgag gccacggugc ggggcgcgaa gcgcauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgcggcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug auguggcugg gccucaacac caagaacggg     2040

agcaucuccc ugaugugccu ggcccucggc ggcgugcuga ucuuccugag cacggccgug     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1206
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long CDS GC

<400>  1206
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agcaagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1207
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long CDS GC

<400>  1207
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

cgccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cagcauccgg      600

ugcaucgggg uguccaaccg cgacuucgug gagggcauga gcggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac gguguccaac auggccgagg ugcggagcua cugcuacgag      780

gcguccauca gcgacauggc cuccgacagc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcggaccc ugguggaccg cggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgggcga aggucgagau cacgccgaac agcccccgcg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cggaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cggcucucca gcgggcaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggc     1500

guguccuaca gccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcgccugau cacggccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1800

agcgggucca cgaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

agcaucuccc ugaugugccu ggcccucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1208
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long CDS GC

<400>  1208
cgcaucauca acgcccggaa ggagaagaag cgccggagcg ccgacaccuc cgugggcauc       60

gucgggcugc uccugaccac ggcgauggcc gccgagguga cccgccgggg cagcgccuac      120

uacauguacc uggaccgcaa cgacgccggg gaggcgaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc cacgaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacggcacg ugccaccaca agaaggggga ggcccggcgc      360

agccggcgcg ccgucacccu ccccucccac agcacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gcuucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg guccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cagcauccgc      600

ugcaucggcg uguccaaccg ggacuucgug gaggggauga gcggcggcac cugggucgac      660

guggugcugg agcacggggg cugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac gguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gcguccauca gcgacauggc cagcgacucc cggugcccca cccaggggga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggc      900

aacgggugcg gccuguucgg gaagggcucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccgggaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cggcaugauc gugaacgaca ccgggcacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucggcggg     1140

uucggcuccc uggggcugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggcgc cgacacgggg acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg cagccaggag     1380

ggggccgucc acaccgcgcu ggccggcgcc cuggaggccg agauggacgg ggccaagggc     1440

cgccucucca gcggccaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggg     1500

guguccuaca gccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ugacggucga ggugcaguac gccgggaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacac ccagacccug acgcccgugg gccggcugau caccgccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacuccu acaucgucau cggcgugggg gagaagaaga ucacgcacca cuggcaccgc     1800

agcggcagca ccaucgggaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccugggcg acaccgccug ggacuucggg uccgugggcg gggcccucaa cagccugggc     1920

aaggggaucc accagaucuu cggcgccgcg uucaaguccc uguucggcgg gaugagcugg     1980

uucucccaga uccugaucgg cacgcuccug auguggcugg ggcucaacac caagaacggc     2040

agcaucuccc ugaugugccu ggcccucggg ggcgugcuga ucuuccugag caccgccguc     2100

uccgccgacg ugggcugcag cguggacuuc uccaag                               2136


<210>  1209
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long CDS GC

<400>  1209
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccaa cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg gagcgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgaucu ccuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgucacccu ccccucccac agcacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac gguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gcguccauca gcgacauggc cuccgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggucca cgaucggcaa ggccuucgag gccaccgugc ggggcgcccg ccggauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

agcaucuccc ugaugugccu ggcccucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1210
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long CDS GC

<400>  1210
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgagaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1211
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long CDS GC

<400>  1211
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cucccugcgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcucg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccug      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cuggugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccucga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcuc     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcuggg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cucgaggccg agauggacgg cgccaagggc     1440

cgccugagcu ccgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc     1500

gugagcuacu cccucugcac cgcggccuuc accuucacca agauccccgc cgagacccug     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcucau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccucgggg acaccgccug ggacuucggc agcgucgggg gcgcccugaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg gacccugcug auguggcucg gccugaacac gaagaacggg     2040

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1212
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long CDS GC

<400>  1212
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccaa cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg gagcgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgaucu ccuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgucacccu ccccucccac agcacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggauguuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac gguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gcguccauca gcgacauggc cuccgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggucca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

agcaucuccc ugaugugccu ggcccucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1213
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long CDS GC

<400>  1213
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggauccug      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcuccuggg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cuccugaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcucg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccug      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cuggugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccucga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcuc     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcuggg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cucgaggccg agauggacgg cgccaagggc     1440

cgccugagcu ccgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc     1500

gugagcuacu cccucugcac cgcggccuuc accuucacca agauccccgc cgagacccug     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcucau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cgggaucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

gugcucgggg acaccgccug ggacuucggc agcgucgggg gcgcccugaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg gacccugcug auguggcucg gccugaacac gaagaacggg     2040

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1214
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long CDS GC

<400>  1214
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucagcaccac cguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gcgacgaucu ccgacaucgc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgccg ucgugcucgg gucccaggag     1380

ggcgcggugc acaccgcccu ggccggggcc cuggaggccg agauggacgg cgcgaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggugga gguccaguac gccggcaccg acggccccug caaggugccg     1620

gcgcagaugg ccguggacau gcagacccug acccccgucg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgcgaa gcgcauggcc     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgcggcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1215
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long CDS GC

<400>  1215
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gacccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acgcccgugg ggcggcugau caccgccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacgcacca cuggcaccgc     1800

agcgggucca ccaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacgcuccug auguggcugg gccucaacac caagaacggg     2040

agcaucuccc ugaugugccu ggcccucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1216
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long CDS GC

<400>  1216
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga gcugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cgguggacau gcagacccug acccccgucg ggcggcucau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

gugcucgggg acaccgccug ggacuucggc agcgucgggg gcgcccugaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg gacccugcug auguggcucg gccugaacac gaagaacggg     2040

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1217
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long CDS GC

<400>  1217
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgagaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcaucgagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ucagcaaccg ggacuucgug gagggcaugu ccggcgggac cuggguggac      660

gucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac ggucagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga agguggagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgugg ucgugcucgg gucccaggag     1380

ggcgccgugc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggugga gguccaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cgguggacau gcagacccug acccccgucg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1218
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long CDS GC

<400>  1218
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcau cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccacggugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac caagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc cacggccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1219
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long CDS GC

<400>  1219
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgugaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgccgcguuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ucacggugga ggugcaguac gccggcaccg acggccccug caaggucccg     1620

gcccagaugg ccguggacau gcagacccug acccccgugg ggcggcugau cacggcgaac     1680

cccgucauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgucc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcu ucaugugccu ggcgcugggc ggcgugcuca ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaag                               2136


<210>  1220
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long CDS GC

<400>  1220
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

aagcucagcu ccgggcaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcgccugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1221
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long CDS GC

<400>  1221
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggaggccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1222
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long CDS GC

<400>  1222
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcgccg acggccccug caaggugccg     1620

gcgcagaugg ccgucgacau gcagacccug acccccgugg ggcggcugau caccgccaac     1680

cccgugauca cggagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccacggugc ggggcgcgaa gcgcauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgcggcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac caagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc cacggccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1223
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long CDS GC

<400>  1223
cgcaucauca acgccggcaa ggagaagaag cggcgcgggg ccgacaccaa cgugggcauc       60

gucgggcugc uccugaccac ggcgauggcc gccgagguga cccggcgcgg cagcgccuac      120

uacauguacc uggaccggaa cgacgccggg gaggcgaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc cgaugcugga cgagggggug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

agccgccggg ccgucacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucaucc ggguggagaa cuggaucuuc      480

cgcaaccccg gcuucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg guccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cagcauccgg      600

ugcaucggcg uguccaaccg cgacuucgug gagggcauga gcgggggcac cugggucgac      660

guggugcugg agcacggggg cugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac gguguccaac auggccgagg ugcggagcua cugcuacgag      780

gcguccauca gcgacauggc cuccgacagc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcggaccc ugguggaccg cggguggggc      900

aacgggugcg gccuguucgg gaagggcucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccggau caugcugagc     1020

guccacgggu cccagcacag cggcaugauc gugaacgaca ccgggcacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccgcg ccgaggccac ccucggcggg     1140

uucggcuccc ugggccugga cugcgagccc cggaccgggc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggcgc cgacacgggg acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcucgg cagccaggag     1380

ggggccgucc acaccgcgcu ggccggcgcc cuggaggccg agauggacgg cgccaagggg     1440

cggcucucca gcggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

guguccuaca gccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ugacggucga ggugcaguac gccggcaccg acgggcccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg gccgccugau cacggccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacuccu acaucgucau cggcgugggg gagaagaaga ucacccacca cuggcaccgg     1800

agcggcucca cgaucggcaa ggccuucgag gccaccgugc gcggggcccg gcgcauggcg     1860

guccugggcg acaccgccug ggacuucggg agcgugggcg gggcccucaa cagccugggc     1920

aagggcaucc accagaucuu cggggccgcg uucaaguccc uguucggcgg gaugagcugg     1980

uucucccaga uccugaucgg cacccuccug auguggcugg ggcucaacac gaagaacggc     2040

agcaucuccc ugaugugccu ggcccucggc ggggugcuga ucuuccugag caccgccguc     2100

uccgccgacg ugggcugcag cguggacuuc uccaag                               2136


<210>  1224
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long CDS GC

<400>  1224
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgagaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa ggacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1225
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long CDS GC

<400>  1225
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggu cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgugugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgucacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacgccgaac agcccccggg cggaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcccug     1320

gucgaguuca aggacgcgca cgccaagcgg cagaccgugg ugguccucgg gucccaggag     1380

ggcgccgugc acaccgcccu ggcgggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgccgcguuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ucacggugga ggugcaguac gccggcaccg acggccccug caaggucccg     1620

gcccagaugg ccguggacau gcagacccug acccccgugg ggcggcugau cacggcgaac     1680

cccgucauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgucc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcgcucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaag                               2136


<210>  1226
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long CDS GC

<400>  1226
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccaa cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg gagcgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgaucu ccuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

augaccucca ccuggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgucacgcu ccccucccac agcacccgga agcugcagac ccgcucccag      420

accuggcugg agagccggga guacacgaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc cacgguggac      720

aucgagcugg ucaccaccac cguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gcguccauca gcgacauggc cuccgacagc cggugcccca cgcagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cgccugcagc      960

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau caccccgaac agcccccggg ccgaggccac gcucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgcggccuuc acguucacca agauccccgc cgagacccuc     1560

cacgggaccg ugaccgucga ggugcaguac gccggcacgg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau caccgccaac     1680

cccgugauca cggaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggucca ccaucggcaa ggccuucgag gccacggugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug auguggcugg gccucaacac caagaacggg     2040

agcaucuccc ugaugugccu ggcccucggc ggcgugcuga ucuuccugag cacggccgug     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1227
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long CDS GC

<400>  1227
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccgc ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc ggacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgcggucc acaccgcccu ggccggggcc cuggaggccg agauggacgg cgcgaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgccgccuuc acguucacca agauccccgc cgagacccuc     1560

cacgggaccg ugaccgucga ggugcaguac gccggcacgg acggccccug caaggugccg     1620

gcgcagaugg ccgucgacau gcagacccug acccccgugg ggcggcugau caccgccaac     1680

cccgugauca cggagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccacggugc ggggcgcgaa gcgcauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgcggcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga cccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1228
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long CDS GC

<400>  1228
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgagaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg cccuggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc gaccguggac      720

aucgagcucg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccug      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cuggugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcucucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgcccaac agcccgcggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccucga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggggc cgacacgggc accccgcacu ggaacaacaa ggaggcgcuc     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcuggg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cucgaggccg agauggacgg cgccaagggc     1440

cgccugagcu ccgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc     1500

gugagcuacu cccucugcac ggcggccuuc accuucacca agauccccgc cgagacccug     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg cggucgacau gcagacccug accccggugg ggcggcucau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccucgggg acaccgccug ggacuucggc agcgucgggg gcgcccugaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg gacccugcug auguggcucg gccugaacac gaagaacggg     2040

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1229
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long CDS GC

<400>  1229
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucggga ucagcaaccg ggacuucgug gagggcaugu ccggcgggac cuggguggac      660

gucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac ggucagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga agguggagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucgggaucc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgugg ucgugcucgg gagccaggag     1380

ggcgccgugc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucuccuaca gccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggugga gguccaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cgguggacau gcagacccug acccccgucg ggcggcugau cacggccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggucca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

agcaucuccc ugaugugccu ggcccucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg ucgggugcag cguggacuuc uccaag                               2136


<210>  1230
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long CDS GC

<400>  1230
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca cgugcgacgc caccaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca cguggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccauccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacgc caagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc cacggccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1231
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long CDS GC

<400>  1231
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccagcgg      360

ucccgccggg ccgucacccu ccccagccac uccacccgca agcugcagac ccggagccag      420

acguggcugg agucccgcga guacaccaag caccucaucc ggguggagaa cuggaucuuc      480

cgcaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgg      600

ugcaucgggg ugagcaaccg cgacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac ggugagcaac auggccgagg ugcgguccua cugcuacgag      780

gcgagcaucu ccgacauggc cagcgacagc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcggaccc ugguggaccg cggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccggau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccgcg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cggaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcucgg gucccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cggcucagcu ccgggcaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggc     1500

gugagcuacu cccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcgccugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1800

uccgggagca cgaucggcaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcg     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1232
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long CDS GC

<400>  1232
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcaggc gcgcagccag      420

accuggcugg agucccggga guacacgaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggccgccgcc gcgaucgccu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc cacgguggac      720

aucgagcugg ucaccaccac cgugagcaac auggcggagg ugcgcuccua cugcuacgag      780

gccagcaucu ccgacauggc cagcgacagc cggugcccca cgcagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugaccu gcgccaaguu cgcgugcucc      960

aagaagauga cgggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau caccccgaac agcccccggg ccgaggccac gcucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc ggacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gucccaggag     1380

ggcgcggucc acaccgcccu ggccggggcc cuggaggccg agauggacgg cgcgaagggc     1440

cgccucagcu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgccgccuuc acguucacca agauccccgc cgagacccuc     1560

cacgggaccg ugaccgucga ggugcaguac gccggcacgg acggccccug caaggugccg     1620

gcgcagaugg ccgucgacau gcagacccug acccccgugg ggcggcugau caccgccaac     1680

cccgugauca cggagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccacggugc ggggcgcgaa gcgcauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgcggcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac caagaacggg     2040

uccaucagcc ugaugugccu ggcccucggc ggcgugcuga ucuuccuguc cacggccgug     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1233
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long CDS GC

<400>  1233
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc gaggcgaucu ccuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgucacccu ccccucccac agcacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccaccac gguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gcguccauca gcgacauggc cagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcga aggucgagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcgcug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcgcu ggccggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgcggccuuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg cggucgacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1860

guccuggggg acaccgccug ggacuucggc uccgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

agcaucuccc ugaugugccu ggcccucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1234
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long CDS GC

<400>  1234
cgcaucauca acgcccggaa ggagaagaag cgccggggca ccgacaccag cguggggauc       60

gucggccugc uccugacgac cgccauggcg gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc gaggccaucu ccuuccccac cacgcucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc gaccaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca cguggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgucacccu ccccucccac agcacccgga agcugcagac gcgcucccag      420

accuggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggcggccgcc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg cgccggccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac gugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gacggacgag     1080

aaccgcgcca aggucgagau caccccgaac agcccccggg cggaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acaccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgccgccuuc accuucacca agauccccgc ggagacgcuc     1560

cacgggaccg ugaccgucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg ccgucgacau gcagacgcug acccccgugg ggcggcugau caccgccaac     1680

cccgugauca ccgagggcac ggagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacagcu acaucgucau cggcgugggg gagaagaaga ucacccacca cuggcaccgc     1800

uccggcagca ccaucggcaa ggcguucgag gccaccgugc gcggggccaa gcggauggcc     1860

guccugggcg acacggccug ggacuucggg agcgugggcg gggugcucaa cucccugggc     1920

aagggcaucc accagaucuu cggggcggcc uucaagagcc uguucggcgg gauguccugg     1980

uucagccaga uccugaucgg cacccuccug auguggcugg ggcucaacac caagaacggc     2040

uccaucagcc ugaugugccu ggcccucggc gggguccuga ucuuccuguc caccgccgug     2100

agcgcggacg ugggcugcuc cgucgacuuc agcaag                               2136


<210>  1235
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long CDS GC

<400>  1235
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcaa cgacgccggc gaggcgauca gcuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccagca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

ucccggcgcg ccgucacccu ccccagccac uccacccgga agcugcagac ccgcagccag      420

acguggcugg agucccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcgcu ggccgccgcc gccaucgcgu ggcugcucgg cagcuccacc      540

agccagaagg ugaucuaccu ggucaugauc cugcucaucg ccccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacg gccauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac ggucagcaac auggcggagg ugcgcuccua cugcuacgag      780

gccagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugacgu gcgccaaguu cgcgugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

gugcacggca gccagcacuc cgggaugcuc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacgccgaac agcccccggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccuguac     1200

uaccucacca ugaacaacaa gcacuggcug gcccacaagg agugguucca cgacaucccc     1260

cugccguggc acgcgggggc cgccacgggc acgccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgg cagaccgugg ugguccuggg gucccaggag     1380

ggcgccgugc acaccgcccu ggccggggcg cucgaggccg agauggacgg cgccaagggc     1440

cgccugagcu ccgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc     1500

gugagcuacu cccucugcac cgccgccuuc accuucacca agauccccgc ggagaccguc     1560

gacgggaccg ugacggugga gggccaguac ggcgggaccg acggccccug caaggucccg     1620

gcccagaugg ccguggacau gcagacccug acccccgugg ggcggcugau cacggccaac     1680

cccgucauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcagca cgaucgggaa ggccuucgag gcgaccgucc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcgcgcucaa cucccugggc     1920

aaggggaucc accagaucau cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggc     2040

uccaucagcc ugaugugccu ggcccucggg ggcgugcuga ucuuccuguc caccgccgug     2100

agcgggggcg uggggugcuc cgucgacuuc agcaag                               2136


<210>  1236
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long CDS GC

<400>  1236
cgcaucauca acgcccggaa ggagaagaag cgccggggca ccgacaccag cguggggauc       60

gucggccugc uccugacgac cgccauggcg gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc gaggccaucu ccuuccccac cacgcucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc gaccaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca cguggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgucacccu ccccucccac agcacccgga agcugcagac gcgcucccag      420

accuggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggcggccgcc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg cgccggccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac gugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gacggacgag     1080

aaccgcgcca aggucgagau caccccgaac agcccccggg cggaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgcgca cgccaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcccu ggcgggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgccgcguuc accuucucca agauccccgc cgagacccuc     1560

cacgggaccg ugacggagga gguccaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg ccguggacau gcagacccug acccccgucg ggcggcugau cacggcgaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggucca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgucgggg gcgcccucaa cucccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccuccug auguggcugg gccucaacac gaagaacggg     2040

uccaucagcc ugaugugccu ggcgcucggc ggcgugcuga ucuuccuguc caccgccgug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1237
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long CDS GC

<400>  1237
cgcaucauca acgcccggaa ggagaagaag cgccggggca ccgacaccag cguggggauc       60

gucggccugc uccugacgac cgccauggcg gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc gaggccaucu ccuuccccac cacgcucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc gaccaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca cguggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgucacccu ccccucccac agcacccgga agcugcagac gcgcucccag      420

accuggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggcggccgcc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg cgccggccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac gugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gacggacgag     1080

aaccgcgcca aggucgagau caccccgaac agcccccggg cggaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgcgca cgccaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcccu ggcgggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgccgcguuc accuucacca agauccccgc cgagacccuc     1560

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg ccgucgacau gcagacccug acgcccgugg ggcggcugau caccgcgaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacgcacca cuggcaccgc     1800

agcgggagca ccaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcc     1860

guccuggggg acaccgcgug ggacuucggc uccgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacgcuccug auguggcugg gccucaacac caagaacggg     2040

agcaucuccc ugaugugccu ggcgcucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1238
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long CDS GC

<400>  1238
cgcaucauca acgcccggaa ggagaagaag cgccggggca ccgacaccag cguggggauc       60

gucggccugc uccugacgac cgccauggcg guggagguga cccgccgggg gaacgccuac      120

uacauguacc uggaccgcuc cgacgccggc gaggccauca gcuuccccac cacgaugggg      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc cgaugcugga cgagggcguc gagcccgacg acguggacug cuggugcaac      300

accaccagca cguggguggu cuacgggacc ugccaccaca agaagggcga ggcgcggcgc      360

ucccggcgcg ccgugacccu gcccagccac uccacccgga agcuccagac gcgcagccag      420

accuggcugg agucccggga guacaccaag caccugaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu cgccgcggcc gccaucgccu ggcugcuggg cagcuccacc      540

agccagaagg ucaucuaccu cgugaugauc cugcugaucg ccccggcgua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucguc gagggcaugu ccggcgggac guggguggac      660

gugguccucg agcacggcgg gugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcugg ugaccacgac cgugagcaac auggccgagg uccgcuccua cugcuacgag      780

gccagcaucu ccgacauggc cagcgacagc cggugcccca cccagggcga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc ucguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccucg aguaccgcau caugcugucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgcgcca agguggagau caccccgaac agcccccggg ccgaggcgac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc uggacuucag cgaccucuac     1200

uaccugacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cucccguggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

gucgaguuca aggacgccca cgcgaagcgg cagaccgugg ugguccuggg gucccaggag     1380

ggcgccgugc acaccgcccu cgccggggcg cuggaggccg agauggacgg cgccaagggc     1440

cgccugagcu ccgggcaccu caagugccgg cugaagaugg acaagcugcg ccucaagggc     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccug     1560

cacgggacgg ucaccgugga ggugcaguac gccggcaccg acggccccug caaggucccg     1620

gcccagaugg ccguggacau gcagacccuc acgcccgugg ggcggcugau caccgccaac     1680

cccgucauca ccgagagcac cgagaacucc aagaugaugc uggagcucga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggugggc gagaagaaga ucacgcacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggcguucgag gccaccgucc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcgcgcugaa cucccucggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacgcugcuc gucuggcugg gccugaacac caagaacggg     2040

uccaucagcc ucaugugccu ggcccugggc ggcgugcuca ucuuccuguc caccgcggug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1239
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long CDS GC

<400>  1239
cgcaucauca acgcccggaa ggagaagaag cgccggggca ccgacaccag cguggggauc       60

gucggccugc uccugacgac cgccauggcg gccgagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc gaggccaucu ccuuccccac cacgcucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc gaccaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca cguggguggu guacgggacc ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgucacccu ccccucccac agcacccgga agcugcagac gcgcucccag      420

accuggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggcggccgcc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg cgccggccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac gugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gacggacgag     1080

aaccgcgcca aggucgagau caccccgaac agcccccggg cggaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgcgca cgccaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcccu ggcgggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgccgcguuc accuucacca agauccccgc cgagacccuc     1560

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg ccgucgacau gcagacccug acgcccgugg ggcggcugau caccgcgaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacgcacca cuggcaccgc     1800

agcgggagca ccaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcc     1860

guccuggggg acaccgcgug ggacuucggc uccgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacgcuccug auguggcugg gccucaacac caagaacggg     2040

agcaucuccc uggugugccu ggcgcucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg uggggugcag cgucgacuuc uccaag                               2136


<210>  1240
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long CDS GC

<400>  1240
cgcaucauca acgcccggaa ggagaagaag cgccggggca ccgacaccag cguggggauc       60

gucggccugc uccugacgac cgccauggcg guggagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc gaggccaucu ccuuccccac cacgcucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc cgaugcugga cgagggcguc gagcccgacg acguggacug cuggugcaac      300

accaccucca cguggguggu cuacgggacc ugccaccaca agaagggcga ggcgcggcgc      360

agccggcgcg ccgugacccu ccccucccac agcacccgga agcugcagac gcgcucccag      420

accuggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggccgcggcc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ucaucuaccu ggugaugauc cugcucaucg ccccggcgua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucguc gagggcauga gcggcgggac guggguggac      660

gugguccugg agcacggcgg gugcgugacc gugauggccc aggacaagcc cgccgucgac      720

aucgagcugg ugaccaccac gguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgucacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacgccgaac agcccccggg cggaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcccug     1320

gucgaguuca aggacgcgca cgccaagcgg cagaccgugg ugguccucgg gagccaggag     1380

ggcgccgugc acaccgcccu ggcgggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

guguccuaca gccugugcac cgccgcguuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ucacggugga ggugcaguac gccggcaccg acggccccug caaggucccg     1620

gcccagaugg ccguggacau gcagacccug acccccgugg ggcggcugau cacggcgaac     1680

cccgucauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggagca cgaucggcaa ggccuucgag gccaccgucc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgcgug ggacuucggc uccgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug guguggcugg gccucaacac gaagaacggg     2040

agcaucuccc ugaccugccu ggcgcucggc ggcgugcuga ucuuccugag caccgccgug     2100

uccgccgacg ucgggugcag cguggacuuc uccaag                               2136


<210>  1241
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long CDS GC

<400>  1241
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc guggagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc gaggccaucu ccuuccccac cacccucggg      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc gacgaugagc      240

uacgagugcc cgaugcugga cgagggcguc gagcccgacg acguggacug cuggugcaac      300

accaccucca ccuggguggu cuacgggacg ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgugacccu ccccucccac agcacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccucaucc gcguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggcggccgcc gucaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggugaugauc cugcucaucg ccccggcgua cagcauccgc      600

ugcaucgggg ucuccaaccg ggacuucgug gagggcauga gcggcgggac cuggguggac      660

gucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac ggucuccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cagcgacucc cggugcccca cccagggcga ggcguaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacgccgaac agcccccggg ccgaggcgac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgugg ucgugcucgg gagccaggag     1380

ggcgccgugc acaccgcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucuccuaca gccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacgggaccg ugacggugga gguccaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg ccguggacau gcagacccug acccccgucg ggcggcugau cacggccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgc     1800

agcgggagca cgaucggcaa ggcguucgag gccaccgugc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgccug ggacuucggc uccgucgggg gcgcgcucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug guguggcugg gccucaacac gaagaacggg     2040

agcaucuccc ugaccugccu ggcccucggc ggcgugcuga ucuuccugag caccgcgguc     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1242
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long CDS GC

<400>  1242
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc guggagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc gaggccaucu ccuuccccac cacccucggg      180

gucaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc gacgaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgugacccu ccccucccac agcacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggcggccgcc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggugaugauc cugcucaucg cgccggccua cagcauccgc      600

ugcaucgggg ucuccaaccg ggacuucgug gagggcauga gcggcgggac cuggguggac      660

gucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac ggucuccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggagau cacgccgaac agcccccggg cggaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacacgggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgcgca cgccaagcgg cagaccgugg ucgugcucgg gagccaggag     1380

ggcgccgugc acaccgcccu ggcgggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucuccuaca gccugugcac cgccgcguuc accuucacca agauccccgc cgagacccuc     1560

cacgggaccg ugacggugga gguccaguac gccggcaccg acggccccug caaggugccg     1620

gcccagaugg ccguggacau gcagacccug acccccgucg ggcggcugau cacggcgaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggggucggc gacaagaaga ucacccacca cugguaccgc     1800

agcgggagca cgaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgcgug ggacuucggc uccgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug guguggcugg gccucaacac gaagaacggg     2040

agcaucuccc ugaccugccu ggcgcucggc ggcgugcuga ucuuccugag caccgccguc     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1243
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long CDS GC

<400>  1243
cgcaucauca acgcccggaa ggagaagaag cgccggggcg ccgacaccag cguggggauc       60

gucggccugc uccugaccac ggcgauggcc guggagguga cccgccgggg guccgccuac      120

uacauguacc uggaccgcag cgacgccggc aaggccaucu ccuuccccac cacccucggg      180

gucaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc gacgaugagc      240

uacgagugcc cgaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga ggcccggcgc      360

agccggcgcg ccgugacccu ccccucccac agcacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggcggccgcc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggugaugauc cugcucaucg cgccggccua cagcauccgc      600

ugcaucgggg ucuccaaccg ggacuucgug gagggcauga gcggcgggac cuggguggac      660

gucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac ggucuccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca agguggagau caccccgaac agcccccggg cggaggccac gcucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgcgca cgccaagcgg cagaccgugg ucgugcucgg gagccaggag     1380

ggcgccgugc acaccgcccu ggcgggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucuccuaca gccugugcac cgccgcguuc acguucacca agauccccgc cgagacccuc     1560

cacgggaccg ugaccgugga gguccaguac gccggcacgg acggccccug caaggugccg     1620

gcccagaugg ccguggacau gcagacccug acccccgucg ggcggcugau caccgcgaac     1680

cccgugauca cggaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggggucggc gacaagaaga ucacccacca cuggcaccgc     1800

agcgggagca ccaucggcaa ggccuucgag gccacggugc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgcgug ggacuucggc uccgucgggg gcgcccucaa cagccugggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg gacccuccug guguggcugg gccucaacac caagaacggg     2040

agcaucuccc ugacgugccu ggcgcucggc ggcgugcuga ucuuccugag caccgccguc     2100

uccgccgacg uggggugcag cguggacuuc uccaag                               2136


<210>  1244
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long CDS GC

<400>  1244
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

gugggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gucaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggucacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca agguggagaa cuggaucuuc      480

cggaaccccg gguucacccu gguggccguc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggugaugauc cugcucaucg cccccgcgua cagcauccgc      600

ugcaucgggg ucuccaaccg ggacuucgug gagggcauga gcggcgggac guggguggac      660

gucgugcugg agcacggcgg gugcgugacc gucauggccc aggacaagcc gaccguggac      720

aucgagcugg ugaccacgac cgucuccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc cagcgacucc cggugcccca cccagggcga ggcguaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggaggu cacccccaac uccccgcggg ccgaggccac ccucgggggc     1140

uucgggagcc ugggccugga cugcgagccc cgcacgggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgcgggggc cgacaccggc accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagacggucg uggugcucgg gagccaggag     1380

ggcgcggucc acaccgcccu ggccggggcc cuggaggcgg agauggacgg cgccaagggc     1440

cgccucuucu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca aggugcccgc cgagacgcuc     1560

cacgggaccg ucaccgugga ggugcaguac gcgggcaccg acggccccug caaggucccc     1620

gcccagaugg ccguggacau gcagacgcug accccggugg ggcggcugau caccgccaac     1680

cccgucauca ccgagagcac ggagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggugggc gacaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gcgaccgucc ggggcgccaa gcgcauggcc     1860

gugcuggggg acacggccug ggacuucggc agcgucgggg gcguguucaa cucccucggg     1920

aagggcaucc accagaucuu cggcgccgcg uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccugcuc guguggcugg gccugaacac caagaacggg     2040

uccaucagcc ugaccugccu cgcccugggc ggcgugauga ucuuccucuc cacggccguc     2100

agcgccgacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1245
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long CDS GC

<400>  1245
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac caaccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

acgaccucca ccugggucgu guacgggacc ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggugacgcu ccccucccac agcacccgca agcugcagac ccggucccag      420

accuggcugg agagccgcga guacacgaag caccucauca aggucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggccgccgug gccaucgcgu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg cccccgccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc gacgguggac      720

aucgagcugg ucaccaccac cguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gcguccauca gcgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu gacccccaac uccccgcggg cggaggccac gcucgggggc     1140

uucgggagcc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccug     1320

gucgaguuca aggacgcgca cgccaagcgg cagaccgugg ugguccucgg gagccaggag     1380

ggcgccgugc acaccgcccu ggcgggggcc cuggaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgccgcguuc acguucacca agguccccgc cgagacccuc     1560

cacgggaccg ugaccgugga gguccaguac gccggcacgg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug accccggucg ggcggcugau caccgcgaac     1680

cccgugauca cggagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccacggugc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgucgggg gcguguucaa cucccucggg     1920

aagggcgugc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccugcuc gucuggcugg gccugaacac caagaacggg     2040

uccaucagcc ucacgugccu ggcccugggc ggcgugauga ucuuccucuc caccgcggug     2100

agcgccgacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1246
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long CDS GC

<400>  1246
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggugacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu gguggccgug gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ucaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucguc gagggcauga gcggcgggac cuggguggac      660

gugguccugg agcacggcgg gugcgugacg gugauggccc aggacaagcc gaccgucgac      720

aucgagcugg ugaccaccac gguguccaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgucacgu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

gugcacggcu cccagcacag cgggaugauc gucaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca agguggaggu gacccccaac uccccgcggg ccgaggcgac ccucgggggc     1140

uucgggagcc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggggc cgacacgggc accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgugg ucgugcucgg gagccaggag     1380

ggcgccgugc acaccgcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucagcuacu cccugugcac ggccgccuuc accuucacca aggugcccgc ggagacccuc     1560

cacgggaccg ugacggucga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccgucgacau gcagacccug accccggugg ggcggcugau cacggccaac     1680

cccgugauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggggugggc gacaagaaga ucacccacca cuggcaccgc     1800

uccgggagca cgaucggcaa ggcguucgag gccaccgugc ggggcgccaa gcgcauggcc     1860

guccuggggg acaccgccug ggacuucggc agcgucgggg gcguguucaa cucccucggg     1920

aagggcaucc accagaucuu cggcgcggcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccugcuc guguggcugg gccugaacac gaagaacggg     2040

uccaucagcc ugaccugccu cgcccugggc ggcgugauga ucuuccucuc caccgccgug     2100

agcgcggacg ucgggugcuc cguggacuuc agcaag                               2136


<210>  1247
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long CDS GC

<400>  1247
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac caaccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

acgaccucca ccugggucgu guacgggacc ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggugacgcu ccccucccac agcacccgca agcugcagac ccggucccag      420

accuggcugg agagccgcga guacacgaag caccucauca aggucgagaa cuggaucuuc      480

cggaaccccg gguucgcccu ggccgccgug gccaucgcgu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggucaugauc cugcucaucg cccccgccua cagcauccgc      600

ugcaucgggg uguccaaccg ggacuucgug gagggcauga gcggcgggac cugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc gacgguggac      720

aucgagcugg ucaccaccac cguguccaac auggccgagg ugcgcagcua cugcuacgag      780

gcguccauca gcgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugaccu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgacg agaaccgcgc caaggucgag     1080

gugaccccca acuccccgcg ggcggaggcc acccucggcg gguucggcag ccuggggcug     1140

gacugcgagc cccgcacggg ccucgacuuc uccgaccugu acuaccugac caugaacaac     1200

aagcacuggc ucgugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc uggucgaguu caaggacgcg     1320

cacgccaagc ggcagacggu ggugguccuc ggcagccagg agggggccgu gcacaccgcc     1380

cuggcgggcg cccuggaggc cgagauggac ggggccaagg gccgccucuu cuccggccac     1440

cugaagugcc ggcugaagau ggacaagcuc cgccugaagg gggugagcua cucccugugc     1500

accgccgcgu ucaccuucac gaaggucccc gccgagaccc uccacggcac cgugaccgug     1560

gagguccagu acgccgggac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagacgc ugaccccggu cggccggcug aucaccgcga accccgugau caccgagagc     1680

acggagaacu ccaagaugau gcucgagcug gacccgcccu ucggggacag cuacaucgug     1740

aucggcgucg gggacaagaa gaucacccac cacuggcacc gcuccggcag caccaucggg     1800

aaggccuucg aggccaccgu gcggggcgcc aagcgcaugg ccgugcuggg cgacacggcg     1860

ugggacuucg ggagcgucgg cgggguguuc aacucccucg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc gggauguccu gguucagcca gauccugauc     1980

ggcacccugc ucguguggcu ggggcugaac accaagaacg gcuccaucag ccugaccugc     2040

cuggcccucg ggggcgucau gaucuuccuc uccacggcgg ugagcgccga cgugggcugc     2100

uccgucgacu ucagcaag                                                   2118


<210>  1248
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long CDS GC

<400>  1248
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

gugggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gucaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggucacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca agguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu gguggccguc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgc      600

ugcaucgggg ucuccaaccg ggacuucgug gagggcauga gcggcgggac cuggguggac      660

gucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc gaccguggac      720

aucgagcugg ugaccaccac ggucuccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca agguggaggu cacccccaac uccccgcggg ccgaggcgac ccucgggggc     1140

uucgggagcc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggggc cgacaccggc accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acacggcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca aggugcccgc ggagacccuc     1560

cacgggacgg ucaccgugga ggugcaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgccggugg ggcggcugau caccgccaac     1680

cccgucauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggugggc gacaagaaga ucacgcacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggcguucgag gccaccgucc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcguguucaa cucccucggg     1920

aagggcaucc accagaucuu cggcgcggcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacgcugcuc guguggcugg gccugaacac caagaacggg     2040

uccaucagcc ugaccugccu cgcccugggc ggcgugauga ucuuccucuc caccgccguc     2100

agcgcggacg uggggugcuc cguggacuuc agcaag                               2136


<210>  1249
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long CDS GC

<400>  1249
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

gugggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gucaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacgggacg ugccagcaca agaagggcga ggcccgccgg      360

agccgccggg cggucacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca agguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu gguggccguc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgc      600

ugcaucgggg ucuccaaccg ggacuucgug gagggcauga gcggcgggac cuggguggac      660

gucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc gaccguggac      720

aucgagcugg ugaccaccac ggucuccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca agguggaggu cacccccaac uccccgcggg ccgaggcgac ccucgggggc     1140

uucgggagcc ugggccugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggggc cgacacgggc accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgucg uggugcucgg gagccaggag     1380

ggcgccgucc acaccgcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gugagcuacu cccugugcac ggccgugugc accgccgcga agguccccgc cgagacccuc     1560

cacgggaccg ugaccgugga gguccaguac gccggcacgg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug accccggucg ggcggcugau caccgcgaac     1680

cccgugauca cggagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggccuucgag gccacggugc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgucgggg gcguguucaa cucccucggg     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccugcuc guguggcugg gccugaacac caagaacggg     2040

uccaucagcc ucacgugccu ggcccugggc ggcgucauga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaag                               2136


<210>  1250
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long CDS GC

<400>  1250
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacgcgag caucgggauc       60

gugucccugc uccugaccac cgucauggcc gccgagauca cgcggcgcgg cagcgccuac      120

uacauguacc uggaccgguc cgacgccggg aaggcgauca gcuucgugac cacccucggc      180

gugaacaagu gccacgucca gaucauggac cuggggcaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

acgaccagca ccugggucgu guacgggacc ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacgcu ccccagccac uccacccgca agcugcagac ccggagccag      420

accuggcugg agucccgcga guacacgaag caccucauca aggucgagaa cuggaucuuc      480

cggaaccccg gcuucgcgcu gguggccgug gccaucgccu ggcugcucgg gagcuccacc      540

agccagaagg ucaucuaccu ggugaugauc cugcucaucg cccccgcgua cuccauccgc      600

ugcaucggcg ugagcaaccg ggacuucguc gaggggaugu ccggcggcac cuggguggac      660

gugguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc gacggucgac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg uccgcuccua cugcuacgag      780

gccagcauca gcgacauggc cuccgacagc cggugcccca cgcaggggga ggcguaccuc      840

gacaagcagu ccgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggc      900

aacgggugcg gccuguucgg gaagggcagc cucgugaccu gcgccaaguu cacgugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

gugcacggca gccagcacuc cggcaugauc gucaacgacg agaaccgcgc caagguggag     1080

gugaccccca acagcccgcg ggccgaggcc acccucgggg gcuucggguc ccugggccug     1140

gacugcgagc cccgcacggg gcucgacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccugcccug gcacuccggc     1260

gcggacaccg agaccccgca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaagc ggcagacggu ggucgugcuc ggcagccagg agggggccgu gcacaccgcg     1380

cuggccggcg cccuggaggc cgagauggac ggggcgaagg gccgccucuc cagcgggcac     1440

cugaagugcc ggcugaagau ggacaagcuc cgccugaagg gcgucuccua cagccugugc     1500

accgccgccu ucaccuucac gaaggugccc gccgagaccc uccacggcac cgugaccguc     1560

gaggugcagu acgccgggcg ggacggcccc ugcaaggugc ccgcgcagau ggccgucgac     1620

augcagaccc ugacgccggu ggggcgccug aucaccgcca accccgugau caccgagucc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacag cuacaucguc     1740

aucggcgugg gggacaagaa gaucacgcac cacuggcacc gguccggcag caucaucggg     1800

aaggccuucg aggccaccgu gcgcggcgcg aagcggaugg ccguccuggg ggacaccgcc     1860

ugggacuucg gcuccguggg cgggguguuc aacagccucg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaaguc ccuguucggg ggcaugagcu gguucuccca gauccugauc     1980

ggcacccugc ucgucuggcu ggggcugaac acgaagaacg gcagcaucuc ccugaccugc     2040

cuggcgcucg ggggcgugau gaucuuccuc agcaccgccg uguccgccga cgucgggugc     2100

agcguggacu ucuccaag                                                   2118


<210>  1251
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long CDS GC

<400>  1251
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

gugggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gucaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccuggguggu guacgggacg ugccaccaca agaagggcga ggcccgccgg      360

agccgccggg cggucacccu ccccucccac agcacccgca agcugcagac ccggucccag      420

acguggcugg agagccgcga guacaccaag caccucauca agguggagaa cuggaucuuc      480

cggaaccccg gguucgcccu gguggccguc gccaucgccu ggcugcucgg cuccagcacc      540

ucccagaagg ugaucuaccu ggugaugauc cugcucaucg cgcccgccua cagcauccgc      600

ugcaucgggg ucuccaaccg ggacuucgug gagggcauga gcggcgggac cuggguggac      660

gucgugcugg agcacggcgg gugcgugacg gucauggccc aggacaagcc gaccguggac      720

aucgagcugg ugaccaccac ggucuccaac auggccgagg ugcgcagcua cugcuacgag      780

gccuccauca gcgacauggc gagcgacucc cggugcccca cccagggcga ggccuaccuc      840

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggucc cucgugacgu gcgccaaguu cacgugcagc      960

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcugagc     1020

guccacggcu cccagcacag cgggaugauc gugaacgacg agaaccgcgc caagguggag     1080

gucaccccca acuccccgcg ggccgaggcg acccucggcg gguucggcag ccuggggcug     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccugu acuaccugac caugaacaac     1200

aagcacuggc ucgugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg ggacgccgca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgcgaagc ggcagaccgu cguggugcuc ggcagccagg agggggccgu ccacaccgcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccgccucuu cuccggccac     1440

cugaagugcc ggcugaagau ggacaagcuc cgccugaagg gggugagcua cucccugugc     1500

accgccgccu ucacguucac caaggugccc gcggagaccc uccacggcac cgucaccgug     1560

gaggugcagu acgccgggac ggacggcccc ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugaccccggu gggccggcug aucaccgcca accccgucau cacggagagc     1680

accgagaacu ccaagaugau gcucgagcug gacccgcccu ucggggacag cuacaucgug     1740

aucggcgugg gggacaagaa gaucacccac cacuggcacc gcuccggcag caccaucggg     1800

aaggcguucg aggccacggu ccggggcgcc aagcgcaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg ggagcguggg cggggucuuc aacucccucg gcaaggggau ccaccagauc     1920

uucggcgcgg ccuucaagag ccuguucggc gggauguccu gguucagcca gauccugauc     1980

ggcacccugc ucguguggcu ggggcugaac accaagaacg gcuccaucag ccucacgugc     2040

cuggcccucg ggggcgugau gaucuuccug uccaccgccg ucagcgcgga cgugggcugc     2100

uccguggacu ucagcaag                                                   2118


<210>  1252
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long CDS GC

<400>  1252
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac cacccucggg      180

gugaacaagu gccacgucca gaucauggac cugggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagccggacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga gacccgccgg      360

agccgccggu ccgugagccu ccgcuaccac uacacccgga agcugcagac ccgcucccag      420

acguggcugg agagccggga guacaagaag caccucauca uggucgagaa cuggaucuuc      480

cgcaaccccg gguucgccau cguguccgug gcgaucaccu ggcugauggg cagccugacc      540

ucccagaagg ucaucuaccu cgugaugauc gugcugaucg uccccgccua cagcaucucc      600

ugcaucgggg ugagcaaccg ggaccuggug gagggcaugu ccggcgggac cugggucgac      660

guggugcucg agcacggcgg gugcgucacg gagauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaugac cgucagcaac auggccgagg ugcgcuccua cugcuacgag      780

gccagccuga gcgacauggc guccgccagc cggugcccga cgcagggcga gcccucccuc      840

gacaagcaga gcgacaccca guccgugugc aagcgcaccc ugggcgaccg gggguggggc      900

aacgggugcg gcaucuucgg gaagggcagc cuggugaccu gcuccaaguu cacgugcugc      960

aagaagaugc ccggcaagag cauccagccc gagaaccucg aguaccgcau caugcugccg     1020

guccacgggu cccagcacag cggcaugauc gucaacgaca ucgggcacga gaccgacgag     1080

aaccgcgcca agguggaggu gacccccaac agcccccggg ccgaggccac ccugggcggg     1140

uucggcuccc ucggccugga cugcgagccc cgcacggggc uggacuucuc cgaccucuac     1200

uaccugacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg     1260

cuccccuggc acgcgggcgc cgacaccggg acgccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgugg ucgugcuggg cagccaggag     1380

ggggcggugc acaccgcccu cgccggcgcc cuggaggccg agauggacgg cgcgaagggg     1440

cgccuguucu ccggccaccu caagugccgg cugaagaugg acaagcugcg ccucaagggg     1500

gucagcuacu cccugugcac cgccgccuuc accuucacga aggugcccgc cgagacccug     1560

cacggcaccg ugaccgucga ggugcagagc gcgggcaccg acgggccgug caaggugccc     1620

gcccagaugg ccgucgacau gcagacgcuc acccccgugg gccggcugau caccgccaac     1680

cccgugauca ccgaguccac ggagaacagc aagaugaugc uggagcucga cccgcccuuc     1740

ggggacagcu acaucgucau cggcgugggg gacaagaaga ucacccacca cuggcaccgc     1800

uccggcagca ccaucggcaa ggccuucgag gcgaccgugc gcggggccaa gcggauggcc     1860

guccugggcg acacggccug ggacuucggg uccgugggcg ggguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggggccgcg uucaaguccc ucuucggcgg gaugagcugg     1980

uucucccaga uccugaucgg cacccugcug gucuggcucg ggcugaacac caagaacggc     2040

agcaucuccc ugaccugccu cgcccugggc ggggugauga ucuuccugag cacggccgug     2100

uccgccgacg ucggcugcag cguggacuuc uccaag                               2136


<210>  1253
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long CDS GC

<400>  1253
cgcaucauca acgcccggaa ggagcgcaag cggcgcggcg ccgacaccag caucgggauc       60

aucggccugc uccugaccac ggcgauggcc gccgagauca cccggcgcgg guccgccuac      120

uacauguacc uggaccggag cgacgccggc aaggcgaucu ccuucgccac caccaugggg      180

gugaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc cacgaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagccggacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacg ugccaccaca agaagggcga ggcccgcccc      360

agccggcgcg cggugacccu gcccucccac agcacccgga agcuccagac ccgcucccag      420

acguggcugg agagccggga guacaccaag caccugauca aggucgagaa cuggauccuc      480

cgcaaccccg gguucaccgu ggccgcccug gccauggccu ggcugcucgg caacuccacc      540

agccagcggg ugaucuaccu ggucaugauc cugcucaucg cgccggccua cuccauccgc      600

ugcaucgggg ugagcaaccg ggacuucgug gagggcaugu ccggcgggac gugggucgac      660

guggugcugg agcacggcgg gugcgucacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ucaccacgac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      780

gccagcaucu ccgacauggc gagcgacagc cggugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      900

aacggcugcg ggcuguucgg caaggggagc cucgugaccu gcgccaaguu cacgugcucc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguaccgcau caugcugucc     1020

guccacggca gccagcacuc cgggaugauc gugaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu gaccccgaac agcccccggg ccgaggcgac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcaccggcc ucgacuucag cgaccuguac     1200

uaccugacga ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugccguggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcccug     1320

gucgaguuca aggacgccca cgcgaagcgg cagaccgugg ugguccucgg gucccaggag     1380

ggcgccgugc acaccgcccu ggccggggcg cuggaggccg agauggacgg cgccaagggc     1440

cgccucuuca gcgggcaccu gaagugccgg aucaagaugg acaagcugcg ccucaagggc     1500

guguccuaca gccugugcac cgccgccuuc accuucucca agguccccgc ggagacccug     1560

cacgggaccg ugacggugga gguccaguac gccggccggg acggccccug caaggugccg     1620

gcccagaugg ccguggacau gcagacccuc acccccgucg ggcgccugau caccgccaac     1680

cccaucauca cggagagcac cgagaacucc cggaugaugc uggagcucga cccgcccuuc     1740

ggcgacagcu acaucgugau cggggugggc gacaagaaga ucacccacca cuggcaccgc     1800

uccgggagca ccaucggcaa ggcguucgag gccacggucc ggggcgccaa gcgcauggcc     1860

gugcuggggg acaccgccug ggacuucggc agcgucgggg gcguguucaa cucccugggg     1920

aagggcgugc accagguguu cggcgcggcc uucaagagcc ucuucggggg cauguccugg     1980

uucagccaga uccugaucgg gacccugcug gucuggcucg gccugaacac caagaacggg     2040

uccaucagcc uggccugccu cgcccugggc ggcgcgauga ucuuccuguc cacggccgug     2100

agcgccgacg uggggugcuc cgucgacuuc                                      2130


<210>  1254
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short CDS GC

<400>  1254
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      660

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1620

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1255
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short CDS GC

<400>  1255
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacacgugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac gagcaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac gcgcaagcug      360

cagacccgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccacgagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacg accaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acgcaguacg ucugcaagcg cacccuggug      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugaa cgacacgggc     1020

cacgagaccg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac gggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac gguggucgug     1320

cugggcuccc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cgcuccacgg caccgugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cgcugacccc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcacggaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccgugcu gggggacacg gcgugggacu ucggcagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacgccaaga acggcuccau cagccugaug ugccucgcgc ugggcggcgu gcugaucuuc     2040

cucuccaccg ccgugagcgc c                                               2061


<210>  1256
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short CDS GC

<400>  1256
accagcaucg ggaucaucgg ccugcugcuc acgaccgcca uggcggccga gaucacccgc       60

cggggguccg ccuacuacau guaccuggac cgcagcgacg ccggcaaggc caucuccuuc      120

gcgaccacgc ugggcgugaa caagugccac gugcagauca uggaccucgg gcacaugugc      180

gacgccacca ugagcuacga gugcccgaug cuggacgagg gcgucgagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccacgugg guggucuacg ggaccugcca ccacaagaag      300

ggcgaggccc ggcgcagccg gcgcgccgug acccugcccu cccacagcac ccggaagcuc      360

cagacgcgcu cccagaccug gcuggagagc cgcgaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuuccggaa ccccggcuuc gcccucgucg cgguggccau cgccuggcug      480

cuggggucca gcaccuccca gaaggugauc uaccucguca ugauccugcu gaucgccccg      540

gccuacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

gggacguggg ucgacguggu gcucgagcac ggcggcugcg ucaccgugau ggcgcaggac      660

aagcccaccg uggacaucga gcuggucacc acgaccgugu ccaacauggc cgagguccgc      720

agcuacugcu acgaggccag caucuccgac auggccagcg acucccggug ccccacccag      780

ggggaggccu accuggacaa gcagagcgac acccaguacg ugugcaagcg cacgcucgug      840

gaccggggcu gggggaacgg cugcgggcug uucggcaagg gcucccuggu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ugagcgugca cggcucccag cacagcggga ugaucggcua cgagacggac     1020

gaggaccggg ccaaggugga ggucaccccg aacuccccgc gcgccgaggc cacccugggc     1080

ggguucggca gccucgggcu ggacugcgag ccccggaccg gccuggacuu cuccgaccuc     1140

uacuaccuga cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccucccgu ggcacgcggg ggccgacacc ggcaccccgc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag cgccagaccg ucguggugcu gggcagccag     1320

gagggggcgg uccacacggc ccuggccggc gcccucgagg ccgagaugga cggggcgaag     1380

ggccggcugu ucuccgggca ccugaagugc cgccucaaga uggacaagcu gcggcugaag     1440

ggcgugagcu acucccucug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc cugcaagauc     1560

ccgguccaga uggcggugga caugcagacc cugacgcccg uggggcgccu caucaccgcc     1620

aaccccguca ucaccgagag caccgagaac uccaagauga ugcuggagcu ggacccgccg     1680

uucggcgaca gcuacaucgu gaucggggug ggcgacaaga agaucacgca ccacuggcac     1740

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc gaagcggaug     1800

gccgugcucg gggacaccgc cugggacuuc ggcagcgugg gcggcgucuu caacucccug     1860

gggaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1920

ugguucagcc agauccucau cgggacgcug cugguguggc ucggccugaa caccaagaac     1980

ggcuccauca gccugaccug ccucgcgcug ggcggcguga ugaucuuccu guccaccgcc     2040

gucagcgcc                                                             2049


<210>  1257
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short CDS GC

<400>  1257
accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg       60

cgcggcuccg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      120

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      360

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      420

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagcccaccg uggacaucga gcucgugacc acgaccguca gcaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacgcuggug      840

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggg aagagcaucc agccggagaa ccuggaguac      960

cgcaucaugc ucuccgucca cggcagccag cacuccggga ugaucgugaa cgacaccggc     1020

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcgcacgcca agcgccagac cguggucgug     1320

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggugacc guggaggucc aguacgccgg gaccgacggc     1560

ccgugcaagg ugcccgccca gauggccgug gacaugcaga cccugacgcc cgucgggcgc     1620

cugaucaccg cgaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ucggggagaa gaagaucacg     1740

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     1980

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu gcugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1258
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short CDS GC

<400>  1258
accagcaucg gcaucaucgg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgugaa caagugccac guccagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccgug acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggugg cgguggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggucauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgugucc aaccgcgacu ucgucgaggg gaugagcggc      600

ggcaccuggg uggacguggu ccuggagcac gggggcugcg ugacggugau ggcgcaggac      660

aagccgaccg ucgacaucga gcuggugacc accacggugu ccaacauggc cgagguccgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacccuggug      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgugca cggcucccag cacagcggca ugaucgucaa cgacaccggg     1020

uacgagaccg acgagaaccg cgccaaggug gaggugaccc ccaacucccc gcgggccgag     1080

gccacccucg gcggguucgg cagccugggg cuggacugcg agccccgcac cggccucgac     1140

uucuccgacc uguacuaccu gacgaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgcggaca ccgggacccc gcacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcggcagac cguggucgug     1320

cucggcagcc aggagggggc cgugcacacg gcgcuggccg gcgcccugga ggccgagaug     1380

gacggggcga agggcaagcu guucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccgccuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccuccacgg cacggugacc gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaaga uccccgugca gauggcgguc gacaugcaga cccugacgcc ggugggccgg     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggacaa gaagaucacg     1740

caccacuggc accgcuccgg cagcaccauc gggaaggccu ucgaggccac cgugcggggc     1800

gccaagcgca uggcgguccu gggcgacacc gccugggacu ucgggagcgu gggcggggug     1860

uucaacuccc ucggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcacgc ugcucgucug gcuggggcug     1980

aacaccaaga acggcuccau cagccugacc ugccucgccc ucgggggcgu gaugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1259
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short CDS GC

<400>  1259
accagcaucg gcaucaucgg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgugaa caagugccac guccagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg gcacgugcca ccggaagaag      300

ggggaggccc gccggagccg ccgggccgug acccuccccu cccacagcac ccgcaagcug      360

cagacccggu cccagacgug gcuggagagc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggugg cgguggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggucauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccgcugcau cggcgugucc aaccgggacu ucgucgaggg gaugagcggc      600

ggcaccuggg uggacguggu ccuggagcac gggggcugcg ugacggugau ggcgcaggac      660

aagccgaccg ucgacaucga gcuggugacc accacggugu ccaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgugca cggcucccag cacagcggca ugaucgucaa cgacaccggg     1020

uacgagaccg acgagaaccg ggccaaggug gaggugaccc ccaacucccc gcgcgccgag     1080

gccacccucg gcggguucgg cagccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gacgaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgcggaca ccgggacccc gcacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcgccagac cguggucgug     1320

cucggcagcc aggagggggc cgugcacacg gcgcuggccg gcgcccugga ggccgagaug     1380

gacggggcga agggcaagcu guucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccuccacgg cacggugacc gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaaga uccccgugca gauggcgguc gacaugcaga cccugacgcc ggugggccgc     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggacaa gaagaucacg     1740

caccacuggc accgguccgg cagcaccauc gggaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggcgguccu gggcgacacc gccugggacu ucgggagcgu gggcggggug     1860

uucaacuccc ucggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcacgc ugcucgucug gcuggggcug     1980

aacaccaaga acggcuccau cagccugacc ugccucgccc ucgggggcgu gaugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1260
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short CDS GC

<400>  1260
accagcaucg gcaucaucgg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgugaa caagugccac guccagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccgug acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggugg cgguggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggucauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgugucc aaccgcgacu ucgucgaggg gaugagcggc      600

ggcaccuggg uggacguggu ccuggagcac gggggcugcg ugacggugau ggcgcaggac      660

aagccgaccg ucgacaucga gcuggugacc accacggugu ccaacauggc cgagguccgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacccuggug      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgugca cggcucccag cacagcggca ugaccgucaa cgacaucggg     1020

uacgagaccg acgagaaccg cgccaaggug gaggugaccc ccaacucccc gcgggccgag     1080

gccacccucg gcggguucgg cagccugggg cuggacugcg agccccgcac cggccucgac     1140

uucuccgacc uguacuaccu gacgaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgcggaca ccgggacccc gcacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcggcagac cguggucgug     1320

cucggcagcc aggagggggc cgugcacacg gcgcuggccg gcgcccugga ggccgagaug     1380

gacggggcga agggcaagcu guucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccgccuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccuccacgg cacggugacc gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaaga uccccgugca gauggcgguc gacaugcaga cccugacgcc ggugggccgg     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggacaa gaagaucacg     1740

caccacuggc accgcuccgg cagcaccauc gggaaggccu ucgaggccac cgugcggggc     1800

gccaagcgca uggcgguccu gggcgacacc gccugggacu ucgggagcgu gggcggggug     1860

uucaacuccc ucggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcacgc ugcucgucug gcuggggcug     1980

aacaccaaga acggcuccau cagccugacc ugccucgccc ucgggggcgu gaugaucuuc     2040

cucuccaccg cggugagcgc c                                               2061


<210>  1261
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short CDS GC

<400>  1261
accagcaucg gcaucguggg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac gugcagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaaggug      420

gagaacugga ucuuccgcaa ccccggcuuc acccuggugg ccgucgcgau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgucucc aaccgcgacu ucguggaggg gaugagcggc      600

ggcacguggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagccgaccg uggacaucga gcuggugacc acgaccgucu ccaacauggc ggaggugcgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacgcugguc      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gaccugcgcc      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacgagaac     1020

cgcgcgaagg uggaggucac ccccaacucc ccgcgggccg aggccacccu cgggggcuuc     1080

gggagccugg gccuggacug cgagccccgc accgggcucg acuucuccga ccuguacuac     1140

cugaccauga acaacaagca cuggcucgug cacaaggagu gguuccacga caucccccug     1200

cccuggcacg ccggcgccga cacgggcacc ccgcacugga acaacaagga ggcgcuggug     1260

gaguucaagg acgcccacgc caagcggcag accgucgugg ugcucgggag ccaggagggc     1320

gccguccaca ccgcccuggc gggggcccug gaggccgaga uggacggcgc caaggggcgc     1380

cucuucuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggcgug     1440

agcuacuccc ugugcacggc ggccuucacc uucaccaagg ugcccgccga gacccuccac     1500

gggaccguca cgguggaggu gcaguacgcc ggcaccgacg ggcccugcaa gguccccgcc     1560

cagauggcgg uggacaugca gacccugacc ccggugggcc gccugaucac ggccaacccc     1620

gucaucaccg agagcaccga gaacuccaag augaugcucg agcuggaccc gcccuucggc     1680

gacagcuaca ucgugaucgg ggugggcgac aagaagauca cccaccacug gcaccggucc     1740

gggagcacga ucggcaaggc cuucgaggcc accguccgcg gggccaagcg gauggcggug     1800

cugggcgaca ccgccuggga cuucggcagc gucgggggcg uguucaacuc ccucgggaag     1860

ggcauccacc agaucuucgg ggccgccuuc aagagccugu ucggcggcau guccugguuc     1920

agccagaucc ugaucgggac ccugcucgug uggcugggcc ugaacacgaa gaacgggucc     1980

aucagccuga ccugccucgc ccugggcggg gugaugaucu uccucuccac cgcggucagc     2040

gcc                                                                   2043


<210>  1262
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short CDS GC

<400>  1262
accagcaucg gcaucaucgg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgugaa caagugccac guccagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccgug acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggugg cgguggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggucauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgugucc aaccgcgacu ucgucgaggg gaugagcggc      600

ggcaccuggg uggacguggu ccuggagcac gggggcugcg ugacggugau ggcgcaggac      660

aagccgaccg ucgacaucga gcuggugacc accacggugu ccaacauggc cgagguccgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacccuggug      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgugca cggcucccag cacagcggca ugaucgggua cgagaccgac     1020

gagaaccgcg ccaaggucga ggugaccccc aacuccccgc gggccgaggc cacccucggc     1080

ggguucggca gccuggggcu ggacugcgag ccccgcaccg gccucgacuu cuccgaccug     1140

uacuaccuga ccaugaacaa caagcacugg cucgugcaca aggagugguu ccacgacauc     1200

ccccugcccu ggcacgccgg cgcggacacg gggaccccgc acuggaacaa caaggaggcc     1260

cuggucgagu ucaaggacgc ccacgccaag cggcagaccg uggugguccu cggcagccag     1320

gagggggccg ugcacaccgc gcuggccggc gcccuggagg ccgagaugga cggggcgaag     1380

ggcaagcugu ucuccggcca ccugaagugc cgccugaaga uggacaagcu ccgccugaag     1440

ggggugagcu acucccugug cacggccgcc uucaccuuca ccaagguccc cgccgagacc     1500

cuccacggca ccgugacggu ggagguccag uacgccggga ccgacggccc cugcaagauc     1560

cccgugcaga uggcggugga caugcagacc cugaccccgg ucggccggcu gaucacggcc     1620

aaccccguga ucaccgagag caccgagaac uccaagauga ugcucgagcu ggacccgccc     1680

uucggggaca gcuacaucgu gaucggcguc ggggacaaga agaucaccca ccacuggcac     1740

cgcuccggca gcacgaucgg gaaggccuuc gaggccaccg ugcggggcgc caagcgcaug     1800

gcggugcugg gcgacaccgc cugggacuuc gggagcgucg gcgggguguu caacucccuc     1860

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg cgggaugucc     1920

ugguucagcc agauccugau cggcacccug cucguguggc uggggcugaa cacgaagaac     1980

ggcuccauca gccugaccug ccucgcccuc gggggcguca ugaucuuccu cuccaccgcg     2040

gugagcgcc                                                             2049


<210>  1263
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short CDS GC

<400>  1263
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggccgggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1264
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short CDS GC

<400>  1264
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggccug gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguca ugauccuccu gaucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcucgucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccuc uucgggaagg gcagccuggu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc ucuccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cugggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcucga ggccgagaug     1380

gacggggcca agggccgccu gagcuccggc caccugaagu gccggcucaa gauggacaag     1440

cugcgccuga agggggugag cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccugcacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cucaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcuggag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cugaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacacgaaga acggcuccau cagccugaug ugccucgccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1265
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short CDS GC

<400>  1265
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggu accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1266
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short CDS GC

<400>  1266
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacacccaga cccugacgcc cgugggccgg     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accgcuccgg cagcaccauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcacgc uccugaugug gcuggggcuc     1980

aacaccaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1267
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short CDS GC

<400>  1267
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgagauccgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ugugcaagcg cacccugguc      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgugca cgggagccag cacuccggca ugaucgucaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cguggucgug     1320

cucggcuccc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg guggaggucc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucgggagcgu cggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggugagcgc c                                               2061


<210>  1268
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short CDS GC

<400>  1268
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugccccaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugcuggccca gauggccguc gacaugcaga cccugacccc ggugggccgg     1620

cucaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcuggag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cugaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacacgaaga acggcuccau cagccugaug ugccucgccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1269
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short CDS GC

<400>  1269
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgacc cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcacgugg gugguguacg ggaccugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacgcgca gccagaccug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcacguggg ucgacguggu gcuggagcac gggggcugcg ucaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc acgaccguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacgcuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cggcagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagacgg acgagaaccg ggccaagguc gagaucaccc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gacgaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca ccgggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggugacc gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacgcc cgugggccgg     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accgcuccgg cagcaccauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcacgc uccugaugug gcuggggcuc     1980

aacaccaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1270
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short CDS GC

<400>  1270
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg ucgugagcgc g                                               2061


<210>  1271
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short CDS GC

<400>  1271
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcgcgugg gugguguacg ggaccugcca ccacaagaag      300

ggcgaggccc ggcgcucccg gcgcgccguc acgcucccca gccacuccac ccggaagcug      360

cagacccgcc cccagaccug gcuggagagc cgggaguaca cgaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa cccgggcuuc gcccuggcgg ccgccgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguca ugauccugcu caucgcgccc      540

gccuacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucaccgugau ggcccaggac      660

aagcccacgg uggacaucga gcuggucacc accaccgugu ccaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggcguccg acagccggug ccccacgcag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugacgggg aaguccaucc agccggagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucaccc ccaacagccc ccgcgcggag     1080

gccacgcucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcgcacgcca agcgccagac cgucguggug     1320

cucggcagcc aggagggggc cguccacacc gcccuggcgg gcgcccugga ggccgagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga aggggguguc cuacagccug ugcaccgccg cguucacguu caccaagauc     1500

cccgccgaga cccuccacgg caccgugacc gucgaggugc aguacgccgg gacggacggc     1560

ccgugcaagg ugcccgccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucaccg cgaaccccgu gaucacggag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcagcgg cuccaccauc gggaaggccu ucgaggccac ggugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gcgugggacu ucgggagcgu gggcggggcc     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacaccaaga acggcagcau cucccugaug ugccuggcgc ucgggggcgu gcugaucuuc     2040

cugagcacgg ccgucuccgc c                                               2061


<210>  1272
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short CDS GC

<400>  1272
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcaa gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1273
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short CDS GC

<400>  1273
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccacccgcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacagca uccggugcau cggcgugucc aaccgcgacu ucguggaggg gaugagcggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacggugu ccaacauggc cgaggugcgg      720

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccgcug ccccacccag      780

ggggaggcgu accucgacaa gcagagcgac acccaguacg ucugcaagcg gacccuggug      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcc      900

aaguucgccu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg cgccaagguc gagaucacgc cgaacagccc ccgggccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccgcac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcagcc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccggcu cuccagcggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga aggggguguc cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgc     1620

cugaucacgg ccaaccccgu gaucaccgag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accggagcgg cuccacgauc gggaaggcgu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcagcau cucccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cugagcaccg cggucuccgc c                                               2061


<210>  1274
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short CDS GC

<400>  1274
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcaccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga aggggguguc cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg cacggugacc gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacgcc cgugggccgg     1620

cugaucaccg ccaaccccgu gaucaccgag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gucaucggcg ugggggagaa gaagaucacg     1740

caccacuggc accgcagcgg cuccaccauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcacgc uccugaugug gcuggggcuc     1980

aacaccaaga acggcagcau cucccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cugagcaccg cggucuccgc c                                               2061


<210>  1275
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short CDS GC

<400>  1275
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacuccc ugcgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcucgucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccuc uucgggaagg gcagccuggu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc ucuccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cugggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcucga ggccgagaug     1380

gacggggcca agggccgccu gagcuccggc caccugaagu gccggcucaa gauggacaag     1440

cugcgccuga agggggugag cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccugcacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cucaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcuggag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cugaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacacgaaga acggcuccau cagccugaug ugccucgccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1276
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short CDS GC

<400>  1276
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcauc      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguga ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugacggucau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accacgguca gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ugugcaagcg cacccugguc      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgugca cgggagccag cacuccggca ugaucgucaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cguggucgug     1320

cucggcuccc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggucag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg guggaggucc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucgggagcgu cggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggugagcgc c                                               2061


<210>  1277
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short CDS GC

<400>  1277
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga uccugcggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcuc      480

cuggggagcu ccaccagcca gaaggugauc uaccugguca ugauccuccu gaucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcucgucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccuc uucgggaagg gcagccuggu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc ucuccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cugggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcucga ggccgagaug     1380

gacggggcca agggccgccu gagcuccggc caccugaagu gccggcucaa gauggacaag     1440

cugcgccuga agggggugag cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccugcacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cucaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcuggag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggca ucggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccgugcu cggcgacacc gccugggacu ucgggagcgu cggcggggcg     1860

cugaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacacgaaga acggcuccau cagccugaug ugccucgccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggugagcgc c                                               2061


<210>  1278
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short CDS GC

<400>  1278
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucagc accaccgugu ccaacauggc cgaggugcgc      720

agcuacugcu acgaggccac gaucuccgac aucgccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgccgucgug     1320

cucggcuccc aggagggggc cgugcacacc gcccuggcgg gcgcccugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggucag cuacucccug ugcaccgccg cguucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgugacg guggaggucc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cugaucacgg cgaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggccu ucgaggccac cgugcggggc     1800

gccaagcgca uggccgugcu gggcgacacc gcgugggacu ucgggagcgu cggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggcgc ucgggggcgu gcugaucuuc     2040

cuguccaccg ccgugagcgc c                                               2061


<210>  1279
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short CDS GC

<400>  1279
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgagcugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cucaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcuggag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccgugcu cggcgacacc gccugggacu ucgggagcgu cggcggggcg     1860

cugaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacacgaaga acggcuccau cagccugaug ugccucgccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggugagcgc c                                               2061


<210>  1280
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short CDS GC

<400>  1280
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaucgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcaccauc gggaaggcgu ucgaggccac ggugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacaccaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccacgg cggucagcgc c                                               2061


<210>  1281
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short CDS GC

<400>  1281
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacgggguga agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacg guggaggugc aguacgcggg gaccgacggc     1560

cccugcaagg ucccggccca gauggccgug gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu caucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggccu ucgaggcgac cguccggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucgggagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg cggccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagcuucaug ugccuggccc ucgggggcgu ccugaucuuc     2040

cuguccaccg ccgugagcgc g                                               2061


<210>  1282
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short CDS GC

<400>  1282
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggcaagcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgc     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgguccgg cagcacgauc gggaaggcgu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1283
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short CDS GC

<400>  1283
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaaggacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1284
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short CDS GC

<400>  1284
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggag gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1285
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short CDS GC

<400>  1285
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcggcccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca ccgggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcgcacgcca agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggcgg gcgcccugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg cguucacguu caccaagauc     1500

cccgccgaga cccuccacgg caccgugacc gucgaggugc aguacgccgg gacggacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucaccg cgaaccccgu gaucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcaccauc gggaaggccu ucgaggccac ggugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gcgugggacu ucgggagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagacccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggcgc ucgggggcgu gcugaucuuc     2040

cuguccaccg ccgucagcgc c                                               2061


<210>  1286
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short CDS GC

<400>  1286
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg ggccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucaccg cgaaccccgu gaucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcaccauc gggaaggccu ucgaggccac ggugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gcgugggacu ucgggagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacaccaaga acggcuccau cagccugaug ugccuggcgc ucgggggcgu gcugaucuuc     2040

cuguccacgg ccgucagcgc c                                               2061


<210>  1287
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short CDS GC

<400>  1287
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugccccaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgcccug      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagccgaccg uggacaucga gcucgucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accuggacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccuc uucgggaagg gcagccuggu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc ucuccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc ccaacagccc gcgcgccgag     1080

gcgacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca cggggacccc gcacuggaac     1260

aacaaggagg cccucgugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cugggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcucga ggccgagaug     1380

gacggggcca agggccgccu gagcuccggc caccugaagu gccggcucaa gauggacaag     1440

cugcgccuga agggggugag cuacucccuc ugcacggccg ccuucaccuu caccaagauc     1500

cccgcggaga cccugcacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugcccgccca gauggccguc gacaugcaga cccugacccc ggugggccgg     1620

cucaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcuggag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cugaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccuc aucggcaccc ugcugaugug gcucgggcug     1980

aacacgaaga acggcuccau cagccugaug ugccucgccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1288
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short CDS GC

<400>  1288
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggucagcg acagccggug ccccacccag      780

ggggaggccu accucgacaa gcaguccgac acccaguacg ugugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu cacgugcgcg      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgugca cgggagccag cacuccggca ugaucgucaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucacgc cgaacagccc ccgcgccgag     1080

gccacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcg ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggucga guucaaggac gcccacgcca agcgccagac cguggugguc     1320

cucggcuccc aggagggggc ggugcacacc gcccuggccg gcgcccugga ggcggagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacg guggaggugc aguacgcggg gaccgacggc     1560

cccugcaagg ucccggccca gauggccgug gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu caucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggccu ucgaggcgac cguccggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucgggagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg cggccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu ccugaucuuc     2040

cuguccaccg ccgugagcgc g                                               2061


<210>  1289
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short CDS GC

<400>  1289
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcaucagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugacggucau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accacgguca gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ugugcaagcg cacccugguc      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgugca cgggagccag cacuccggca ugaucgucaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cauccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cguggucgug     1320

cucggcagcc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggucuc cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg guggaggucc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accgcagcgg cuccacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucgggagcgu cggcggggcg     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcagcau cucccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cugagcaccg cgguguccgc c                                               2061


<210>  1290
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short CDS GC

<400>  1290
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacacgugc      180

gacgccacca uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcacgugg gugguguacg ggaccugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccau ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cggcagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacgccaaga acggcuccau cagccugaug ugccuggcgc ucgggggcgu gcugaucuuc     2040

cuguccacgg ccgucagcgc c                                               2061


<210>  1291
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short CDS GC

<400>  1291
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc agcggucccg ccgggccguc acccucccca gccacuccac ccgcaagcug      360

cagacccgga gccagacgug gcuggagucc cgcgaguaca ccaagcaccu cauccgggug      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccggugcau cggcgugagc aaccgcgacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgg      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccgcug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg gacccuggug      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cggaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg cgccaagguc gagaucacgc cgaacagccc ccgggccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccgcac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcggcagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgc     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgguccgg cagcacgauc gggaaggcgu ucgaggccac cgugcgcggc     1800

gccaagcgga uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1292
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short CDS GC

<400>  1292
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

caggcccgca gccagaccug gcuggagucc cgggaguaca cgaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggcgg ccgccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgcgccg      540

gccuacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucaccgugau ggcccaggac      660

aagcccacgg uggacaucga gcuggucacc accaccguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggcgagcg acagccggug ccccacgcag      780

ggggaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugacgggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucaccc cgaacagccc ccgcgcggag     1080

gccacgcucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca ccgggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcgcacgcca agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggcgg gcgcccugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg cguucacguu caccaagauc     1500

cccgccgaga cccuccacgg caccgugacc gucgaggugc aguacgccgg gacggacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucaccg cgaaccccgu gaucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcaccauc gggaaggccu ucgaggccac ggugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gcgugggacu ucgggagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacaccaaga acggcuccau cagccugaug ugccuggcgc ucgggggcgu gcugaucuuc     2040

cuguccacgg ccgucagcgc c                                               2061


<210>  1293
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short CDS GC

<400>  1293
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccggggaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacggugu ccaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacccag      780

ggggaggcgu accucgacaa gcagagcgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcc      900

aaguucgccu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcagcc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga aggggguguc cuacagccug ugcaccgccg ccuucaccuu cuccaagauc     1500

cccgcggaga cccuccacgg caccgugacc gaggaggucc aguacgccgg gacggacggc     1560

cccugcaagg ugccggccca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cugaucaccg ccaaccccgu gaucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accgcagcgg cuccaccauc gggaaggcgu ucgaggccac ggugcggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucgggagcgu cggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacaccaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccacgg cggugagcgc c                                               2061


<210>  1294
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short CDS GC

<400>  1294
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccggggaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacggugu ccaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacccag      780

ggggaggcgu accucgacaa gcagagcgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcc      900

aaguucgccu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacacg ggcgccgaca ccgggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcgccagac cgucguggug     1320

cucggcagcc aggagggggc gguccacacc gcccuggccg gcgcccugga ggcggagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga aggggguguc cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg cacggugacc gucgaggugc aguacgcggg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacgcc cgugggccgg     1620

cugaucaccg ccaaccccgu gaucaccgag gggaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucggcga cagcuacauc gucaucgggg ugggcgagaa gaagaucacg     1740

caccacuggc accgcuccgg gagcaccauc ggcaaggccu ucgaggcgac cgugcggggc     1800

gccaagcgca uggccguccu gggggacacc gccugggacu ucggcagcgu cgggggcgug     1860

cucaacuccc uggggaaggg cauccaccag aucuucggcg ccgcguucaa gagccuguuc     1920

gggggcaugu ccugguucag ccagauccug aucgggacgc uccugaugug gcugggccuc     1980

aacaccaaga acggguccau cagccugaug ugccuggccc ucggcggcgu gcugaucuuc     2040

cuguccaccg ccgugagcgc c                                               2061


<210>  1295
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short CDS GC

<400>  1295
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccggggaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacggugu ccaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacccag      780

ggggaggcgu accucgacaa gcagagcgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcc      900

aaguucgccu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcagcc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga aggggguguc cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcagcgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucggguccgu gggcggggcg     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcagcau cucccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cugagcaccg cggucuccgc c                                               2061


<210>  1296
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short CDS GC

<400>  1296
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgugga ggugacccgc       60

cggggcaacg cguacuacau guaccuggac cgcuccgacg ccggggaggc caucagcuuc      120

cccaccacca ugggcaugaa caagugcuac auccagauca uggaccucgg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcgucgagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg guggucuacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcucccg gcgcgcggug acccugccca gccacuccac ccggaagcuc      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu gauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccucgccg ccgcggccau cgccuggcug      480

cuggggagcu ccaccagcca gaaggucauc uaccucguga ugauccugcu gaucgccccg      540

gccuacucca uccgcugcau cggcgugagc aaccgggacu ucgucgaggg gauguccggc      600

ggcaccuggg uggacguggu ccucgagcac gggggcugcg ugacggugau ggcgcaggac      660

aagcccaccg ucgacaucga gcuggugacc accacgguga gcaacauggc cgagguccgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggccu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacccucgug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccuggu cacgugcgcg      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccucgaguac      960

cgcaucaugc uguccgugca cgggagccag cacuccggca ugaucgucaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucacgc cgaacagccc ccgcgccgag     1080

gccacccugg gcggguucgg cucccucggg cuggacugcg agccccggac cggccuggac     1140

uucagcgacc ucuacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccuccc guggcacgcg ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggucga guucaaggac gcccacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc ggugcacacc gcccucgccg gcgcccugga ggcggagaug     1380

gacggggcca agggccgccu gagcuccggc caccucaagu gccggcugaa gauggacaag     1440

cugcgccuca agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgucacg guggaggugc aguacgcggg gaccgacggc     1560

cccugcaagg ucccggccca gauggccgug gacaugcaga cccucacccc cgugggccgg     1620

cugaucacgg ccaaccccgu caucaccgag agcaccgaga acuccaagau gaugcuggag     1680

cucgacccgc ccuucgggga cagcuacauc gugaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggccu ucgaggcgac cguccggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucgggagcgu gggcggggcc     1860

cugaacuccc ucggcaaggg gauccaccag aucuucggcg cggccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc ugcucgucug gcuggggcug     1980

aacacgaaga acggcuccau cagccucaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg ccgugagcgc g                                               2061


<210>  1297
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short CDS GC

<400>  1297
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccggggaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacagca uccgcugcau cggcgugucc aaccgggacu ucguggaggg gaugagcggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacggugu ccaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacccag      780

ggggaggcgu accucgacaa gcagagcgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcc      900

aaguucgccu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcagcc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga aggggguguc cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcagcgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucggguccgu gggcggggcg     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcagcau cucccuggug ugccuggccc ucgggggcgu ccugaucuuc     2040

cugagcaccg cgguguccgc c                                               2061


<210>  1298
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short CDS GC

<400>  1298
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccggggaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacagca uccgcugcau cggcgugaac aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccuggug ugccuggccc ucgggggcgu ccugaucuuc     2040

cuguccaccg cggugagcgc c                                               2061


<210>  1299
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short CDS GC

<400>  1299
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgugga ggugacccgc       60

cggggcuccg cguacuacau guaccuggac cgcagcgacg ccggggaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcgucgagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg guggucuacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgcggug acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg ccgcggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggucauc uaccugguga ugauccugcu caucgccccg      540

gccuacagca uccgcugcau cggcgugucc aaccgggacu ucgucgaggg gaugagcggc      600

ggcaccuggg uggacguggu ccuggagcac gggggcugcg ugacggugau ggcgcaggac      660

aagcccgccg ucgacaucga gcuggugacc accaccgugu ccaacauggc cgagguccgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacgcag      780

ggggaggcgu accucgacaa gcagagcgac acccaguacg ugugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugacgggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgucaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucaccc cgaacagccc ccgcgccgag     1080

gcgacgcucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca ccgggacgcc ccacuggaac     1260

aacaaggagg cccuggucga guucaaggac gcccacgcga agcgccagac cguggugguc     1320

cucggcagcc aggagggggc cgugcacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga aggggguguc cuacagccug ugcaccgccg ccuucacguu caccaagauc     1500

cccgcggaga cccuccacgg caccgucacc guggaggugc aguacgccgg gacggacggc     1560

cccugcaagg ucccggccca gauggccgug gacaugcaga cccugacccc cgugggccgg     1620

cugaucaccg ccaaccccgu caucacggag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gugaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcagcgg cagcaccauc gggaaggcgu ucgaggccac gguccggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucggguccgu gggcggggcg     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccuggucug gcuggggcuc     1980

aacaccaaga acggcagcau cucccugacg ugccuggccc ucgggggcgu gcugaucuuc     2040

cugagcaccg cgguguccgc c                                               2061


<210>  1300
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short CDS GC

<400>  1300
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgugga ggugacccgc       60

cggggcuccg cguacuacau guaccuggac cgcagcgacg ccggggaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcgucgagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg guggucuacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgcggug acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg ccgcggucau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguga ugauccugcu caucgccccg      540

gccuacagca uccgcugcau cggcgucucc aaccgggacu ucguggaggg gaugagcggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugacggucau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accacggucu ccaacauggc ggaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg cacccugguc      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcc      900

aaguucgcgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucacgc cgaacagccc ccgcgccgag     1080

gccacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgcggaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcgccagac cguggucgug     1320

cucggcagcc aggagggggc cgugcacacc gcgcuggccg gcgcccugga ggccgagaug     1380

gacggggcga agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggucuc cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgugacg guggaggucc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggcgca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gugaucggcg ucggggagaa gaagaucacc     1740

caccacuggc accgcagcgg cagcacgauc gggaaggccu ucgaggccac cgugcggggc     1800

gcgaagcgca uggccgugcu gggcgacacc gccugggacu ucggguccgu cggcggggcc     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg ccgcguucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccuggugug gcuggggcuc     1980

aacacgaaga acggcagcau cucccugacc ugccuggccc ucgggggcgu gcugaucuuc     2040

cugagcaccg ccgucuccgc c                                               2061


<210>  1301
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short CDS GC

<400>  1301
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgugga ggugacccgc       60

cggggcuccg cguacuacau guaccuggac cgcagcgacg ccggggaggc caucuccuuc      120

cccaccaccc ucggcgucaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgcggug acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg ccgcggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguga ugauccugcu caucgccccg      540

gccuacagca uccgcugcau cggcgucucc aaccgggacu ucguggaggg gaugagcggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugacggucau ggcgcaggac      660

aagcccaccg uggacaucga gcuggugacc accacggucu ccaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg cacccugguc      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcg      900

aaguucgccu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucacgc cgaacagccc ccgcgccgag     1080

gccacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcg ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcgccagac cguggucgug     1320

cucggcagcc aggagggggc ggugcacacc gcccuggccg gcgcccugga ggcggagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggucuc cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgugacg guggaggucc aguacgcggg gaccgacggc     1560

cccugcaagg ugccggccca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gugaucggcg ucggggacaa gaagaucacc     1740

caccacuggu accgcagcgg cagcacgauc gggaaggccu ucgaggcgac cgugcggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucggguccgu cggcggggcc     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg cggccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccuggugug gcuggggcuc     1980

aacacgaaga acggcagcau cucccugacc ugccuggccc ucgggggcgu gcugaucuuc     2040

cugagcaccg ccgucuccgc g                                               2061


<210>  1302
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short CDS GC

<400>  1302
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgugga ggugacccgc       60

cggggcuccg cguacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

cccaccaccc ucggcgucaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga ugagcuacga gugcccgaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgcggug acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg ccgcggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguga ugauccugcu caucgccccg      540

gccuacagca uccgcugcau cggcgucucc aaccgggacu ucguggaggg gaugagcggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugacggucau ggcgcaggac      660

aagcccaccg uggacaucga gcuggugacc accacggucu ccaacauggc cgaggugcgc      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccggug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg cacccugguc      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcg      900

aaguucgccu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaucggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucaccc cgaacagccc ccgcgccgag     1080

gccacgcucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcg ggcgccgaca ccgggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcgccagac cguggucgug     1320

cucggcagcc aggagggggc ggugcacacc gcccuggccg gcgcccugga ggcggagaug     1380

gacggggcca agggccgccu cuccagcggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggucuc cuacagccug ugcaccgccg ccuucacguu caccaagauc     1500

cccgccgaga cccuccacgg caccgugacc guggaggucc aguacgcggg gacggacggc     1560

cccugcaagg ugccggccca gauggccgug gacaugcaga cccugacccc cgucggccgg     1620

cugaucaccg ccaaccccgu gaucacggag uccaccgaga acagcaagau gaugcucgag     1680

cuggacccgc ccuucgggga cuccuacauc gugaucggcg ucggggacaa gaagaucacc     1740

caccacuggc accgcagcgg cagcaccauc gggaaggccu ucgaggcgac ggugcggggc     1800

gccaagcgca uggccgugcu gggcgacacc gccugggacu ucggguccgu cggcggggcc     1860

cucaacagcc ugggcaaggg gauccaccag aucuucggcg cggccuucaa gucccuguuc     1920

ggcgggauga gcugguucuc ccagauccug aucggcaccc uccuggugug gcuggggcuc     1980

aacaccaaga acggcagcau cucccugacg ugccuggccc ucgggggcgu gcugaucuuc     2040

cugagcaccg ccgucuccgc g                                               2061


<210>  1303
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short CDS GC

<400>  1303
accagcaucg gcaucguggg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac gugcagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaaggug      420

gagaacugga ucuuccgcaa ccccggcuuc acccuggugg ccgucgcgau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgucucc aaccgcgacu ucguggaggg gaugagcggc      600

ggcacguggg uggacgucgu gcuggagcac gggggcugcg ugaccgucau ggcccaggac      660

aagccgaccg uggacaucga gcuggugacc acgaccgucu ccaacauggc ggaggugcgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacgcugguc      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gaccugcgcc      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg cgcgaaggug gaggucaccc ccaacucccc gcgggccgag     1080

gccacccucg gcggguucgg cagccugggg cuggacugcg agccccgcac gggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1260

aacaaggagg cgcuggugga guucaaggac gcccacgcca agcggcagac ggucguggug     1320

cucggcagcc aggagggggc cguccacacc gcccuggcgg gcgcccugga ggccgagaug     1380

gacggggcca agggccgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgcgg ccuucaccuu caccaaggug     1500

cccgccgaga cgcuccacgg caccgucacc guggaggugc aguacgccgg gaccgacggc     1560

cccugcaagg uccccgccca gauggcggug gacaugcaga cgcugacccc ggugggccgc     1620

cugaucaccg ccaaccccgu caucaccgag agcacggaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ugggggacaa gaagaucacc     1740

caccacuggc accgguccgg cagcaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gccaagcgga uggcggugcu gggcgacacg gccugggacu ucgggagcgu gggcgggguc     1860

uucaacuccc ucggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc ugcucgugug gcuggggcug     1980

aacaccaaga acggcuccau cagccugacc ugccuggccc ucgggggcgu gaugaucuuc     2040

cucuccacgg cggucagcgc c                                               2061


<210>  1304
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short CDS GC

<400>  1304
accagcaucg gcaucaucgg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaacc ucggcgugaa caagugccac guccagauca uggaccuggg gcacaugugc      180

gacgcgacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacacgac cuccaccugg gucguguacg ggaccugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccgug acgcuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagaccug gcuggagagc cgggaguaca cgaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggcgg ccguggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguca ugauccugcu caucgccccc      540

gcguacagca uccggugcau cggcgugucc aaccgcgacu ucguggaggg gaugagcggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucaccgugau ggcccaggac      660

aagccgacgg uggacaucga gcuggucacc accaccgugu ccaacauggc cgaggugcgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacgcag      780

ggggaggcgu accucgacaa gcagagcgac acccaguacg ucugcaagcg gacccuggug      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gaccugcgcc      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaucggg     1020

cacgagaccg acgagaaccg cgccaagguc gaggugaccc ccaacucccc gcgggccgag     1080

gccacgcucg gcggguucgg cagccugggg cuggacugcg agccccgcac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcg ggcgccgaca ccgggacgcc gcacuggaac     1260

aacaaggagg cccuggucga guucaaggac gcccacgcca agcggcagac cguggugguc     1320

cucggcagcc aggagggggc ggugcacacc gcccuggccg gcgcccugga ggcggagaug     1380

gacggggcca agggccgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucacguu caccaagguc     1500

cccgccgaga cccuccacgg caccgugacc guggaggucc aguacgcggg gacggacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc ggucggccgc     1620

cugaucaccg ccaaccccgu gaucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ucggggacaa gaagaucacc     1740

caccacuggc accgguccgg cagcaccauc gggaaggccu ucgaggcgac ggugcgcggc     1800

gccaagcgga uggccgugcu gggcgacacc gccugggacu ucgggagcgu cggcggggug     1860

uucaacuccc ucggcaaggg ggugcaccag aucuucggcg ccgcguucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc ugcucgucug gcuggggcug     1980

aacaccaaga acggcuccau cagccucacg ugccuggccc ugggcggcgu gaugaucuuc     2040

cucuccaccg ccgugagcgc c                                               2061


<210>  1305
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short CDS GC

<400>  1305
accagcaucg gcaucaucgg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgugaa caagugccac guccagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccgug acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggugg cgguggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggucauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgugucc aaccgcgacu ucgucgaggg gaugagcggc      600

ggcaccuggg uggacguggu ccuggagcac gggggcugcg ugacggugau ggcgcaggac      660

aagccgaccg ucgacaucga gcuggugacc accacggugu ccaacauggc cgagguccgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacccuggug      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu cacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgugca cggcucccag cacagcggca ugaucgucaa cgacaucggg     1020

cacgagaccg acgagaaccg cgccaaggug gaggugaccc ccaacucccc gcgggccgag     1080

gccacccucg gcggguucgg cagccugggg cuggacugcg agccccgcac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgcggaca cggggacccc gcacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcggcagac cguggucgug     1320

cucggcagcc aggagggggc cgugcacacc gcgcuggccg gcgcccugga ggccgagaug     1380

gacggggcga agggccgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggucag cuacucccug ugcacggccg ccuucaccuu caccaaggug     1500

cccgccgaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugcccgcgca gauggccguc gacaugcaga cccugacccc ggugggccgc     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggacaa gaagaucacc     1740

caccacuggc accgguccgg cagcacgauc gggaaggccu ucgaggccac cgugcgcggc     1800

gcgaagcgga uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggug     1860

uucaacuccc ucggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc ugcucgucug gcuggggcug     1980

aacacgaaga acggcuccau cagccugacc ugccuggcgc ucgggggcgu gaugaucuuc     2040

cucuccaccg ccgugagcgc c                                               2061


<210>  1306
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short CDS GC

<400>  1306
accagcaucg gcaucaucgg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaacc ucggcgugaa caagugccac guccagauca uggaccuggg gcacaugugc      180

gacgcgacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacacgac cuccaccugg gucguguacg ggaccugcca ccacaagaag      300

ggcgaggccc ggcgcagccg gcgcgccgug acgcuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagaccug gcuggagagc cgggaguaca cgaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggcgg ccguggccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguca ugauccugcu caucgccccc      540

gcguacagca uccggugcau cggcgugucc aaccgcgacu ucguggaggg gaugagcggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucaccgugau ggcccaggac      660

aagccgacgg uggacaucga gcuggucacc accaccgugu ccaacauggc cgaggugcgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacgcag      780

ggggaggcgu accucgacaa gcagagcgac acccaguacg ucugcaagcg gacccuggug      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gaccugcgcc      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacgagaac     1020

cgcgccaagg ucgaggugac ccccaacucc ccgcgggccg aggccacccu cgggggcuuc     1080

gggagccugg gccuggacug cgagccccgc acggggcucg acuucuccga ccuguacuac     1140

cugaccauga acaacaagca cuggcucgug cacaaggagu gguuccacga caucccccug     1200

cccuggcacg cgggcgccga caccggcacc ccgcacugga acaacaagga ggcccugguc     1260

gaguucaagg acgcccacgc caagcggcag acgguggugg uccucgggag ccaggagggc     1320

gcggugcaca ccgcccuggc cggggcccug gaggcggaga uggacggcgc caaggggcgc     1380

cucuucuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggcgug     1440

agcuacuccc ugugcaccgc cgccuucacc uucacgaagg uccccgccga gacccuccac     1500

gggaccguga ccguggaggu ccaguacgcg ggcaccgacg ggcccugcaa ggugcccgcc     1560

cagauggccg uggacaugca gacgcugacc ccggucggcc gccugaucac cgccaacccc     1620

gugaucaccg agagcacgga gaacuccaag augaugcucg agcuggaccc gcccuucggc     1680

gacagcuaca ucgugaucgg ggucggcgac aagaagauca cccaccacug gcaccggucc     1740

gggagcacca ucggcaaggc cuucgaggcg accgugcgcg gggccaagcg gauggccgug     1800

cugggcgaca cggccuggga cuucggcagc gucgggggcg uguucaacuc ccucgggaag     1860

ggcauccacc agaucuucgg ggccgcguuc aagagccugu ucggcggcau guccugguuc     1920

agccagaucc ugaucgggac ccugcucgug uggcugggcc ugaacaccaa gaacgggucc     1980

aucagccuga ccugccucgc ccugggcggg gucaugaucu uccucuccac ggccgugagc     2040

gcc                                                                   2043


<210>  1307
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short CDS GC

<400>  1307
accagcaucg gcaucguggg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac gugcagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaaggug      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggugg cggucgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgucucc aaccgcgacu ucguggaggg gaugagcggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugacggucau ggcgcaggac      660

aagccgaccg uggacaucga gcuggugacc accacggucu ccaacauggc cgaggugcgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacccugguc      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg cgccaaggug gaggucaccc ccaacucccc gcgggccgag     1080

gccacccucg gcggguucgg cagccugggg cuggacugcg agccccgcac cggccucgac     1140

uucuccgacc uguacuaccu gacgaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgcggaca ccgggacccc gcacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcggcagac cgucguggug     1320

cucggcagcc aggagggggc cguccacacg gcgcuggccg gcgcccugga ggccgagaug     1380

gacggggcga agggccgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1560

cccugcaagg uccccgcgca gauggccgug gacaugcaga cccugacgcc ggugggccgc     1620

cugaucaccg ccaaccccgu caucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ugggggacaa gaagaucacg     1740

caccacuggc accgguccgg cagcaccauc gggaaggccu ucgaggccac cguccgcggc     1800

gcgaagcgga uggccgugcu gggcgacacc gccugggacu ucgggagcgu gggcgggguc     1860

uucaacuccc ucggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcacgc ugcucgugug gcuggggcug     1980

aacaccaaga acggcuccau cagccugacc ugccuggcgc ucgggggcgu gaugaucuuc     2040

cucuccaccg ccgucagcgc c                                               2061


<210>  1308
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short CDS GC

<400>  1308
accagcaucg gcaucguggg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac gugcagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca gcacaagaag      300

ggggaggccc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaaggug      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggugg cggucgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgucucc aaccgcgacu ucguggaggg gaugagcggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugacggucau ggcgcaggac      660

aagccgaccg uggacaucga gcuggugacc accacggucu ccaacauggc cgaggugcgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacccugguc      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacaucggg     1020

cacgagaccg acgagaaccg cgccaaggug gaggucaccc ccaacucccc gcgggccgag     1080

gccacccucg gcggguucgg cagccugggg cuggacugcg agccccgcac cggccucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgcggaca cggggacccc gcacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcggcagac cgucguggug     1320

cucggcagcc aggagggggc cguccacacc gcgcuggccg gcgcccugga ggccgagaug     1380

gacggggcga agggccgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggggugag cuacucccug ugcacggccg ugugcaccgc cgccaagguc     1500

cccgccgaga cccuccacgg caccgugacc guggaggucc aguacgcggg gacggacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc ggucggccgc     1620

cugaucaccg ccaaccccgu gaucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ucggggacaa gaagaucacc     1740

caccacuggc accgguccgg cagcaccauc gggaaggccu ucgaggcgac ggugcgcggc     1800

gccaagcgga uggccgugcu gggcgacacc gccugggacu ucgggagcgu cggcggggug     1860

uucaacuccc ucggcaaggg gauccaccag aucuucggcg ccgcguucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc ugcucgugug gcuggggcug     1980

aacaccaaga acggcuccau cagccucacg ugccuggccc ucgggggcgu caugaucuuc     2040

cuguccaccg ccgugagcgc c                                               2061


<210>  1309
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short CDS GC

<400>  1309
accagcaucg gcaucguggg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac gugcagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggccc ggcgcagccg gcgcgccguc acccuccccu cccacagcac ccggaagcug      360

cagacccgcu cccagacgug gcuggagagc cgggaguaca ccaagcaccu caucaaggug      420

gagaacugga ucuuccgcaa ccccggcuuc gcccuggugg cggucgccau cgccuggcug      480

cucgggucca gcaccuccca gaaggugauc uaccugguga ugauccugcu caucgccccc      540

gccuacagca uccggugcau cggcgucucc aaccgcgacu ucguggaggg gaugagcggc      600

ggcaccuggg uggacgucgu gcuggagcac gggggcugcg ugacggucau ggcgcaggac      660

aagccgaccg uggacaucga gcuggugacc accacggucu ccaacauggc cgaggugcgg      720

agcuacugcu acgaggccuc caucagcgac auggccagcg acucccgcug ccccacccag      780

ggggaggccu accucgacaa gcagagcgac acccaguacg ugugcaagcg gacccugguc      840

gaccgcggcu ggggcaacgg gugcggccug uucgggaagg gcucccucgu gacgugcgcg      900

aaguucacgu gcagcaagaa gaugaccggg aaguccaucc agcccgagaa ccuggaguac      960

cggaucaugc ugagcgucca cggcucccag cacagcggca ugaucgugaa cgacgagaac     1020

cgcgccaagg uggaggucac ccccaacucc ccgcgggccg aggccacccu cgggggcuuc     1080

gggagccugg gccuggacug cgagccccgc accgggcucg acuucuccga ccuguacuac     1140

cugaccauga acaacaagca cuggcucgug cacaaggagu gguuccacga caucccccug     1200

cccuggcacg ccggcgcgga caccggcacg ccgcacugga acaacaagga ggcccuggug     1260

gaguucaagg acgcccacgc caagcggcag accgucgugg ugcucgggag ccaggagggc     1320

gccguccaca ccgcgcuggc cggggcccug gaggccgaga uggacggcgc gaaggggcgc     1380

cucuucuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggcgug     1440

agcuacuccc ugugcaccgc cgccuucacg uucaccaagg ugcccgccga gacccuccac     1500

gggaccguca ccguggaggu gcaguacgcc ggcacggacg ggcccugcaa gguccccgcg     1560

cagauggccg uggacaugca gacccugacc ccggugggcc gccugaucac cgccaacccc     1620

gucaucacgg agagcaccga gaacuccaag augaugcucg agcuggaccc gcccuucggc     1680

gacagcuaca ucgugaucgg ggugggcgac aagaagauca cccaccacug gcaccggucc     1740

gggagcacca ucggcaaggc cuucgaggcc acgguccgcg gggcgaagcg gauggccgug     1800

cugggcgaca ccgccuggga cuucggcagc gucgggggcg uguucaacuc ccucgggaag     1860

ggcauccacc agaucuucgg ggccgccuuc aagagccugu ucggcggcau guccugguuc     1920

agccagaucc ugaucgggac ccugcucgug uggcugggcc ugaacaccaa gaacgggucc     1980

aucagccuca cgugccuggc gcugggcggg gugaugaucu uccucuccac cgccgucagc     2040

gcc                                                                   2043


<210>  1310
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short CDS GC

<400>  1310
accagcaucg gcaucaucgg gcugcuccug accacggcca uggccgcgga gaucacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcagcgacg ccgggaaggc caucuccuuc      120

gccaccaccc ucggcgugaa caagugccac guccagauca uggaccuggg gcacaugugc      180

gacgcgacga ugagcuacga gugccccaug cuggacgagg gcguggagcc ggacgacgug      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg gcacgugcca ccacaagaag      300

ggggagaccc ggcgcagccg gcgcuccgug agccuccggu accacuacac ccgcaagcug      360

cagacccggu cccagacgug gcuggagagc cgcgaguaca agaagcaccu caucaugguc      420

gagaacugga ucuuccggaa ccccggcuuc gccaucgugu ccguggccau caccuggcug      480

auggggagcc ugaccuccca gaaggucauc uaccucguga ugaucgugcu gaucgucccc      540

gccuacagca ucuccugcau cggcgugagc aaccgcgacc ugguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcucgagcac gggggcugcg ucacggagau ggcgcaggac      660

aagcccaccg uggacaucga gcuggugacc augaccguca gcaacauggc cgaggugcgg      720

uccuacugcu acgaggccag ccugagcgac auggccuccg ccagccgcug cccgacgcag      780

ggggagcccu cccucgacaa gcagagcgac acccaguccg ugugcaagcg gacccugggc      840

gaccgcggcu gggggaacgg cugcgggauc uucggcaagg ggagccuggu gaccugcucc      900

aaguucacgu gcugcaagaa gaugcccggc aagagcaucc agcccgagaa ccucgaguac      960

cggaucaugc ugccggugca cggcucccag cacagcggga ugaucgucaa cgacaucggc     1020

cacgagaccg acgagaaccg cgcgaaggug gaggugaccc ccaacagccc ccgggccgag     1080

gccacccucg ggggcuucgg gucccugggc cuggacugcg agccccgcac gggccuggac     1140

uucuccgacc ucuacuaccu gaccaugaac aacaagcacu ggcuggucca caaggagugg     1200

uuccacgaca ucccgcuccc cuggcacgcc ggggccgaca ccggcacgcc ccacuggaac     1260

aacaaggagg cgcuggugga guucaaggac gcccacgcca agcggcagac cguggucgug     1320

cuggggagcc aggagggcgc cgugcacacc gcccucgcgg gggcccugga ggccgagaug     1380

gacggcgcca agggccgccu guucuccggg caccucaagu gccgccugaa gauggacaag     1440

cugcggcuca agggcgucag cuacucccug ugcaccgccg cguucaccuu cacgaaggug     1500

cccgccgaga cccugcacgg gaccgugacc gucgaggugc agagcgccgg caccgacggc     1560

ccgugcaagg ugcccgccca gauggcgguc gacaugcaga cgcucacccc cguggggcgc     1620

cugaucaccg ccaaccccgu gaucaccgag uccacggaga acagcaagau gaugcuggag     1680

cucgacccgc ccuucggcga cagcuacauc gucaucgggg ugggcgacaa gaagaucacc     1740

caccacuggc accgguccgg gagcaccauc ggcaaggccu ucgaggccac cgugcgcggc     1800

gccaagcgga uggcgguccu gggggacacg gccugggacu ucggcuccgu cgggggcgug     1860

uucaacagcc uggggaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

gggggcauga gcugguucuc ccagauccug aucgggaccc ugcuggucug gcucggccug     1980

aacaccaaga acgggagcau cucccugacc ugccucgccc ugggcggcgu gaugaucuuc     2040

cugagcacgg cgguguccgc c                                               2061


<210>  1311
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short CDS GC

<400>  1311
agcgugggca ucgucgggcu gcuccugacc accgccaugg ccgcggaggu gacgcgccgg       60

ggcuccgccu acuacaugua ccuggaccgc aacgacgccg gggaggccau cagcuucccc      120

accacccucg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      180

gccaccaugu ccuacgagug cccgaugcug gacgagggcg uggagcccga cgacgucgac      240

ugcuggugca acacgaccag caccugggug guguacggga ccugccacca caagaagggg      300

gaggcgcggc gcucccggcg cgccgucacg cuccccagcc acuccacccg gaagcugcag      360

acccgcagcc agaccuggcu ggagucccgg gaguacacga agcaccucau ccgcguggag      420

aacuggaucu uccggaaccc cggcuucgcc cuggccgcgg ccgccaucgc cuggcugcuc      480

gggagcucca ccagccagaa ggugaucuac cuggucauga uccugcucau cgccccggcg      540

uacuccaucc gcugcaucgg cgugagcaac cgggacuucg uggaggggau guccggcggc      600

accugggucg acguggugcu ggagcacggg ggcugcguca ccgugauggc ccaggacaag      660

cccacggugg acaucgagcu ggucaccacc accgugagca acauggccga ggugcgcucc      720

uacugcuacg aggccagcau cuccgacaug gccagcgaca gccggugccc cacgcagggg      780

gaggcguacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccugguggac      840

cggggcuggg gcaacgggug cggccuguuc gggaagggca gccucgugac cugcgccaag      900

uucgccugcu ccaagaagau gacgggcaag agcauccagc ccgagaaccu ggaguaccgc      960

aucaugcugu ccguccacgg cagccagcac uccggcauga ucgugaacga caccgggcac     1020

gagaccgacg agaaccgggc caaggucgag aucaccccga acagcccccg cgccgaggcg     1080

acgcucggcg gguucggcuc ccuggggcug gacugcgagc cccggaccgg ccucgacuuc     1140

agcgaccugu acuaccugac caugaacaac aagcacuggc ucgugcacaa ggagugguuc     1200

cacgacaucc cccugccgug gcacgccggc gccgacaccg ggacgcccca cuggaacaac     1260

aaggaggccc ugguggaguu caaggacgcc cacgcgaagc gccagaccgu cguggugcuc     1320

ggcucccagg agggggccgu ccacaccgcc cuggccggcg cgcuggaggc cgagauggac     1380

ggggccaagg gccgccucag cuccggccac cugaagugcc ggcugaagau ggacaagcuc     1440

cgccugaagg gggugagcua cucccugugc accgccgccu ucacguucac caagaucccc     1500

gcggagaccc uccacggcac cgugaccguc gaggugcagu acgccgggac ggacggcccc     1560

ugcaaggugc cggcccagau ggccgucgac augcagaccc ugacccccgu gggccggcug     1620

aucaccgcca accccgugau cacggagagc accgagaacu ccaagaugau gcucgagcug     1680

gacccgcccu ucggggacag cuacaucguc aucggcgugg gggagaagaa gaucacccac     1740

cacuggcacc gcuccggcag caccaucggg aaggcguucg aggccacggu gcggggcgcc     1800

aagcgcaugg ccguccuggg cgacaccgcc ugggacuucg ggagcguggg cggggcgcuc     1860

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1920

gggauguccu gguucagcca gauccugauc ggcacccucc ugauguggcu ggggcucaac     1980

accaagaacg gcuccaucag ccugaugugc cuggcccucg ggggcgugcu gaucuuccug     2040

uccacggcgg ucagcgcc                                                   2058


<210>  1312
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short CDS GC

<400>  1312
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gcccgccgga uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1313
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short CDS GC

<400>  1313
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga uguuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggugau ggcccaggac      660

aagcccaccg uggacaucga gcuggucacc accacgguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacccag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cgggagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucacgc cgaacagccc ccgcgccgag     1080

gcgacccucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca cggggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacg gucgaggugc aguacgccgg gaccgacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu gaucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaggcgu ucgaggccac cgugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccaccg cggucagcgc c                                               2061


<210>  1314
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short CDS GC

<400>  1314
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaugac cagcaccugg gugguguacg ggaccugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acgcucccca gccacuccac ccggaagcug      360

cagacccgca gccagaccug gcuggagucc cgggaguaca cgaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucaccgugau ggcccaggac      660

aagcccacgg uggacaucga gcuggucacc accaccguga gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggccagcg acagccggug ccccacgcag      780

ggggaggcgu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gaccugcgcc      900

aaguucgccu gcuccaagaa gaugacgggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgucca cggcagccag cacuccggca ugaucgugaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaagguc gagaucaccc cgaacagccc ccgcgccgag     1080

gcgacgcucg gcggguucgg cucccugggg cuggacugcg agccccggac cggccucgac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgaca ccgggacgcc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcga agcgccagac cgucguggug     1320

cucggcuccc aggagggggc cguccacacc gcccuggccg gcgcgcugga ggccgagaug     1380

gacggggcca agggccgccu cagcuccggc caccugaagu gccggcugaa gauggacaag     1440

cuccgccuga agggggugag cuacucccug ugcaccgccg ccuucacguu caccaagauc     1500

cccgcggaga cccuccacgg caccgugacc gucgaggugc aguacgccgg gacggacggc     1560

cccugcaagg ugccggccca gauggccguc gacaugcaga cccugacccc cgugggccgg     1620

cugaucaccg ccaaccccgu gaucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gucaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcaccauc gggaaggcgu ucgaggccac ggugcggggc     1800

gccaagcgca uggccguccu gggcgacacc gccugggacu ucgggagcgu gggcggggcg     1860

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacaccaaga acggcuccau cagccugaug ugccuggccc ucgggggcgu gcugaucuuc     2040

cuguccacgg cggucagcgc c                                               2061


<210>  1315
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short CDS GC

<400>  1315
accagcgugg gcaucgucgg gcugcuccug accacggcca uggccgcgga ggugacccgc       60

cggggcuccg ccuacuacau guaccuggac cgcaacgacg ccggggaggc caucagcuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      180

gacgccacga uguccuacga gugcccgaug cuggacgagg gcguggagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcacgugcca ccacaagaag      300

ggggaggcgc ggcgcucccg gcgcgccguc acccucccca gccacuccac ccggaagcug      360

cagacccgca gccagacgug gcuggagucc cgggaguaca ccaagcaccu cauccgcgug      420

gagaacugga ucuuccggaa ccccggcuuc gcccuggccg cggccgccau cgccuggcug      480

cucgggagcu ccaccagcca gaaggugauc uaccugguca ugauccugcu caucgccccg      540

gcguacucca uccgcugcau cggcgugagc aaccgggacu ucguggaggg gauguccggc      600

ggcaccuggg ucgacguggu gcuggagcac gggggcugcg ucacggccau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accacgguca gcaacauggc cgaggugcgc      720

uccuacugcu acgaggccag caucuccgac auggcgagcg acagccggug ccccacccag      780

ggggaggccu accucgacaa gcaguccgac acccaguacg ugugcaagcg cacccugguc      840

gaccggggcu ggggcaacgg gugcggccug uucgggaagg gcagccucgu gacgugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

cgcaucaugc uguccgugca cgggagccag cacuccggca ugcucgucaa cgacaccggg     1020

cacgagaccg acgagaaccg ggccaaggug gagaucacgc cgaacagccc ccgcgcggag     1080

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1140

uucagcgacc uguacuaccu caccaugaac aacaagcacu ggcuggccca caaggagugg     1200

uuccacgaca ucccccugcc guggcacgcc ggcgccgcga cggggacgcc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cguggugguc     1320

cugggcuccc aggagggggc ggugcacacc gcccuggccg gcgcccucga ggccgagaug     1380

gacggggcga agggccgccu gagcuccggc caccugaagu gccggcucaa gauggacaag     1440

cugcgccuga agggggugag cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga ccgucgacgg caccgugacg guggaggggc aguacggcgg caccgacggg     1560

cccugcaagg ucccggccca gauggcggug gacaugcaga cccugacccc cgugggccgg     1620

cugaucacgg ccaaccccgu caucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucgggga cagcuacauc gugaucggcg ugggggagaa gaagaucacc     1740

caccacuggc accgcuccgg cagcacgauc ggcaaggccu ucgaggccac cguccgcggg     1800

gccaagcgga uggcggugcu gggcgacacc gccugggacu ucgggagcgu gggcggggcc     1860

cucaacuccc ugggcaaggg cauccaccag aucaucgggg ccgcguucaa gagccuguuc     1920

ggcgggaugu ccugguucag ccagauccug aucggcaccc uccugaugug gcuggggcuc     1980

aacacgaaga acggcuccau cagccugaug ugccuggccc ucggcggggu ccugaucuuc     2040

cuguccaccg ccgugagcgg c                                               2061


<210>  1316
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short CDS GC

<400>  1316
ggcgccgacg ccagcaucgg gaucgugucc cugcuccuga ccaccgucau ggcggccgag       60

aucacgcgcc ggggcagcgc cuacuacaug uaccuggacc gcuccgacgc cgggaaggcc      120

aucagcuucg ugaccacccu cggcgugaac aagugccacg uccagaucau ggaccugggg      180

cacaugugcg acgcgaccau guccuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacacgacc agcaccuggg ucguguacgg gaccugccac      300

cacaagaagg gggaggcccg gcgcucccgg cgcgccguga cgcuccccag ccacuccacc      360

cggaagcugc agacccgcag ccagaccugg cuggaguccc gggaguacac gaagcaccuc      420

aucaaggucg agaacuggau cuuccgcaac cccggcuucg cccugguggc gguggccauc      480

gccuggcugc ucgggagcuc caccagccag aaggucaucu accuggugau gauccugcuc      540

aucgcccccg ccuacuccau ccggugcauc ggcgugagca accgcgacuu cgucgagggg      600

auguccggcg gcaccugggu ggacgugguc cuggagcacg ggggcugcgu gaccgugaug      660

gcgcaggaca agccgacggu cgacaucgag cuggugacca ccaccgugag caacauggcc      720

gagguccggu ccuacugcua cgaggccagc aucagcgaca uggccuccga cagccgcugc      780

cccacgcagg gggaggccua ccucgacaag caguccgaca cccaguacgu gugcaagcgg      840

acccuggucg accgcggcug gggcaacggg ugcggccugu ucgggaaggg cagccucgug      900

accugcgcga aguucacgug cuccaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguacc ggaucaugcu guccgugcac ggcagccagc acuccggcau gaucgucaac     1020

gacgagaacc gcgccaaggu ggaggugacc cccaacagcc cgcgggccga ggccacccuc     1080

gggggcuucg ggucccuggg ccuggacugc gagccccgca cggggcucga cuucagcgac     1140

cuguacuacc ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1200

aucccccugc ccuggcacuc cggcgccgac accgagaccc cgcacuggaa caacaaggag     1260

gcgcuggugg aguucaagga cgcccacgcc aagcggcaga cgguggucgu gcucggcagc     1320

caggaggggg ccgugcacac cgcccuggcg ggcgcccugg aggccgagau ggacggggcc     1380

aagggccgcc ucuccagcgg gcaccugaag ugccgccuga agauggacaa gcuccggcug     1440

aagggcgucu ccuacagccu gugcaccgcg gccuucaccu ucacgaaggu gcccgccgag     1500

acccuccacg gcaccgugac cgucgaggug caguacgccg ggcgcgacgg ccccugcaag     1560

gugcccgccc agauggcggu cgacaugcag acccugacgc cgguggggcg gcugaucacc     1620

gccaaccccg ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg     1680

cccuucggcg acagcuacau cgucaucggc gugggggaca agaagaucac gcaccacugg     1740

caccgcuccg gcagcaucau cgggaaggcc uucgaggcca ccgugcgggg cgccaagcgc     1800

auggcggucc ugggggacac cgccugggac uucggcuccg ugggcggggu guucaacagc     1860

cucggcaagg ggauccacca gaucuucggc gccgccuuca agucccuguu cgggggcaug     1920

agcugguucu cccagauccu gaucggcacc cugcucgucu ggcuggggcu gaacacgaag     1980

aacggcagca ucucccugac cugccuggcc cucgggggcg ugaugaucuu ccucagcacc     2040

gcgguguccg cc                                                         2052


<210>  1317
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short CDS GC

<400>  1317
ggcgccgaca ccagcaucgg gaucaucggc cugcuccuga ccacggccau ggcggccgag       60

aucacccgcc ggggguccgc cuacuacaug uaccuggacc gcagcgacgc cggcaaggcc      120

aucuccuucg cgaccaccau gggggugaac aagugccacg uccagaucau ggaccucggc      180

cacaugugcg acgccacgau gagcuacgag ugccccaugc uggacgaggg cguggagccg      240

gacgacgugg acugcuggug caacaccacc uccaccuggg ucguguacgg gacgugccac      300

cacaagaagg gcgaggcccg gcccagccgc cgggccguga cccugcccuc ccacagcacc      360

cgcaagcucc agacccgguc ccagacgugg cuggagagcc gcgaguacac caagcaccug      420

aucaaggucg agaacuggau ccuccggaac cccggguuca ccguggcggc ccuggccaug      480

gccuggcugc ucggcaacuc caccagccag cgcgugaucu accuggucau gauccugcuc      540

aucgccccgg cguacuccau ccggugcauc ggggugagca accgcgacuu cguggagggc      600

auguccggcg ggacgugggu cgacguggug cuggagcacg gcgggugcgu caccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggucacca cgaccgugag caacauggcc      720

gaggugcggu ccuacugcua cgaggccagc aucuccgaca uggccagcga cagccgcugc      780

cccacccagg gcgaggcgua ccucgacaag caguccgaca cccaguacgu cugcaagcgg      840

acgcuggugg accgcggcug ggggaacggc ugcgggcugu ucggcaaggg gagccucgug      900

accugcgcca aguucacgug cuccaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguacc ggaucaugcu guccguccac ggcagccagc acuccgggau gaucgugaac     1020

gacaucggcc acgagaccga cgagaaccgc gccaaggucg aggugacccc gaacagcccc     1080

cgggccgagg ccacccucgg gggcuucggg ucccugggcc uggacugcga gccccgcacc     1140

ggccucgacu ucagcgaccu guacuaccug acgaugaaca acaagcacug gcucgugcac     1200

aaggaguggu uccacgacau cccccugccg uggcacgcgg gggccgacac cggcacgccc     1260

cacuggaaca acaaggaggc ccuggucgag uucaaggacg cccacgccaa gcggcagacc     1320

gugguggucc ucggguccca ggagggcgcg gugcacaccg cccuggccgg ggcccuggag     1380

gcggagaugg acggcgccaa gggccgccuc uucagcgggc accugaagug ccgcaucaag     1440

auggacaagc ugcggcucaa gggcgugucc uacagccugu gcaccgccgc cuucaccuuc     1500

uccaaggucc ccgccgagac ccugcacggg accgugacgg uggaggucca guacgcgggc     1560

cgcgacggcc ccugcaaggu gccggcccag auggccgugg acaugcagac ccucaccccc     1620

gucgggcggc ugaucaccgc caaccccauc aucacggaga gcaccgagaa cucccgcaug     1680

augcuggagc ucgacccgcc cuucggcgac agcuacaucg ugaucggggu gggcgacaag     1740

aagaucaccc accacuggca ccgguccggg agcaccaucg gcaaggccuu cgaggcgacg     1800

guccgcggcg ccaagcggau ggccgugcug ggggacaccg ccugggacuu cggcagcguc     1860

gggggcgugu ucaacucccu ggggaagggc gugcaccagg uguucggcgc cgcguucaag     1920

agccucuucg ggggcauguc cugguucagc cagauccuga ucgggacccu gcuggucugg     1980

cucggccuga acaccaagaa cggguccauc agccuggccu gccucgcccu gggcggcgcc     2040

augaucuucc uguccacggc ggugagcgcc                                      2070


<210>  1318
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE CDS GC

<400>  1318
agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc       60

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      120

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      180

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      300

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      360

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      420

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      480

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      600

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      660

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg      720

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag      840

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc      900

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg      960

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1020

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1080

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1140

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1320

gugcuggggg acaccgcgug ggacuucggc agc                                  1353


<210>  1319
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE CDS GC

<400>  1319
agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc       60

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac cuggguggac      120

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      180

aucgagcucg ugacgaccac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      300

gacaagcagu ccgacacgca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      420

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      480

guccacggca gccagcacuc cgggaugauc gugaacgaca cgggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      600

uucggcuccc uggggcucga cugcgagccc cggacgggcc uggacuucag cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg      720

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg cucccaggag      840

ggggccgugc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc      900

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg      960

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacgcuc     1020

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1080

gcccagaugg ccguggacau gcagacgcug acccccgucg ggcgccugau caccgcgaac     1140

cccgugauca ccgagagcac ggagaacagc aagaugaugc ucgagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacccacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1320

gugcuggggg acacggcgug ggacuucggc agc                                  1353


<210>  1320
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE CDS GC

<400>  1320
ucccagaagg ugaucuaccu cgucaugauc cugcugaucg ccccggccua cagcauccgc       60

ugcaucggcg uguccaaccg ggacuucgug gaggggauga gcggcgggac gugggucgac      120

guggugcucg agcacggcgg cugcgucacc gugauggcgc aggacaagcc caccguggac      180

aucgagcugg ucaccacgac cguguccaac auggccgagg uccgcagcua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cccaggggga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcacgc ucguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caagggcucc cuggucacgu gcgcgaaguu cacgugcagc      420

aagaagauga ccgggaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc      480

gugcacggcu cccagcacag cgggaugauc ggcuacgaga cggacgagga ccgggccaag      540

guggagguca ccccgaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccuc      600

gggcuggacu gcgagccccg gaccggccug gacuucuccg accucuacua ccugacgaug      660

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu cccguggcac      720

gcgggggccg acaccggcac cccgcacugg aacaacaagg aggcccuggu ggaguucaag      780

gacgcccacg ccaagcgcca gaccgucgug gugcugggca gccaggaggg ggcgguccac      840

acggcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggccg gcuguucucc      900

gggcaccuga agugccgccu caagauggac aagcugcggc ugaagggcgu gagcuacucc      960

cucugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcacgguc     1020

accguggagg ugcaguacgc cgggaccgac ggccccugca agaucccggu ccagauggcg     1080

guggacaugc agacccugac gcccgugggg cgccucauca ccgccaaccc cgucaucacc     1140

gagagcaccg agaacuccaa gaugaugcug gagcuggacc cgccguucgg cgacagcuac     1200

aucgugaucg gggugggcga caagaagauc acgcaccacu ggcaccggag cggcuccacc     1260

aucgggaagg ccuucgaggc caccguccgc ggcgcgaagc ggauggccgu gcucggggac     1320

accgccuggg acuucggcag c                                               1341


<210>  1321
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE CDS GC

<400>  1321
agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc       60

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      120

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      180

aucgagcucg ugaccacgac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      300

gacaagcagu ccgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      360

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      420

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      480

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gacggacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      600

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      660

uaccucacga ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg      720

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgcgca cgccaagcgc cagaccgugg ucgugcuggg cucccaggag      840

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc      900

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg      960

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1020

cacggcacgg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acgcccgucg ggcgccugau caccgcgaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacgcacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1320

gugcuggggg acaccgcgug ggacuucggc agc                                  1353


<210>  1322
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE CDS GC

<400>  1322
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggcuacga gaccgacgag      540

aaccgggcca agguggaggu cacgcccaac uccccgcgcg ccgaggccac ccucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggggc cgacacgggc accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcuggg gagccaggag      840

ggcgcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg ggcgaagggc      900

aagcuguuca gcgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc      960

guguccuaca gccucugcac ggccgccuuc accuucacca agguccccgc cgagacccug     1020

cacgggacgg ugaccgugga gguccaguac gccggcaccg acggccccug caagaucccc     1080

gugcagaugg cgguggacau gcagacccug acgccggucg ggcggcucau caccgccaac     1140

cccgugauca ccgaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggggucggc gacaagaaga ucacgcacca cuggcaccgc     1260

agcgggucca ccaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1320

gugcucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1323
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE CDS GC

<400>  1323
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugacc gugaacgaca ucggcuacga gaccgacgag      540

aaccgggcca agguggaggu cacgcccaac uccccgcgcg ccgaggccac ccucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggggc cgacacgggc accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcuggg gagccaggag      840

ggcgcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg ggcgaagggc      900

aagcuguuca gcgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc      960

guguccuaca gccucugcac ggccgccuuc accuucacca agguccccgc cgagacccug     1020

cacgggacgg ugaccgugga gguccaguac gccggcaccg acggccccug caagaucccc     1080

gugcagaugg cgguggacau gcagacccug acgccggucg ggcggcucau caccgccaac     1140

cccgugauca ccgaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggggucggc gacaagaaga ucacgcacca cuggcaccgc     1260

agcgggucca ccaucggcaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcg     1320

gugcucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1324
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE CDS GC

<400>  1324
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg gccucuucgg gaaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgacg agaaccgggc caagguggag      540

gucaccccca acuccccgcg cgccgaggcc acccugggcg gguucggcag ccugggccuc      600

gacugcgagc cccggacggg gcuggacuuc uccgaccugu acuaccucac caugaacaac      660

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgcgggc      720

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucguggaguu caaggacgcc      780

cacgccaagc gccagacggu cguggugcug ggcagccagg agggggcggu ccacaccgcc      840

cuggccggcg cccucgaggc cgagauggac ggcgcgaagg ggcggcuguu cagcggccac      900

cugaagugcc gccucaagau ggacaagcug cggcugaagg ggguguccua cagccucugc      960

accgccgccu ucaccuucac gaaggucccc gccgagaccc ugcacggcac cgugaccgug     1020

gagguccagu acgccgggac ggacggcccc ugcaaggugc ccgcgcagau ggccguggac     1080

augcagaccc ugaccccggu cggccgccuc aucaccgcca accccgugau cacggagucc     1140

accgagaaca gcaagaugau gcuggagcug gacccgcccu ucggggacuc cuacaucgug     1200

aucggcgucg gggacaagaa gaucacccac cacuggcacc ggagcggcuc caccaucggg     1260

aaggccuucg aggccacggu gcgcggcgcg aagcggaugg ccgugcucgg cgacaccgcc     1320

ugggacuucg ggagc                                                      1335


<210>  1325
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE CDS GC

<400>  1325
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc ggcuacgaga ccgacgagaa ccgggccaag      540

guggagguga cccccaacuc cccgcgcgcc gaggccacgc ucgggggcuu cggcagccug      600

gggcucgacu gcgagccccg gaccggccug gacuucuccg accuguacua ccucaccaug      660

aacaacaagc acuggcuggu ccacaaggag ugguuccacg acaucccccu gcccuggcac      720

gcgggggccg acaccggcac gccgcacugg aacaacaagg aggcccucgu ggaguucaag      780

gacgcccacg ccaagcgcca gaccgugguc gugcugggga gccaggaggg cgcggugcac      840

accgcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggcaa gcuguucagc      900

gggcaccuga agugccggcu caagauggac aagcugcgcc ugaagggcgu cuccuacagc      960

cucugcaccg ccgccuucac guucaccaag gugcccgccg agacccugca cgggaccguc     1020

acgguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcg     1080

guggacaugc agacccugac cccggugggg cggcucauca cggccaaccc cgucaucacc     1140

gaguccaccg agaacagcaa gaugaugcug gagcuggacc cgcccuucgg cgacuccuac     1200

aucgugaucg gggugggcga caagaagauc acccaccacu ggcaccgcag cggguccacg     1260

aucggcaagg ccuucgaggc caccguccgg ggcgccaagc gcauggcggu gcucggggac     1320

accgccuggg acuucggcag c                                               1341


<210>  1326
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE CDS GC

<400>  1326
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cucccugcgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcucg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcugg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ucguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcugga cugcgagccc cggaccggcc uggacuucuc cgaccucuac      660

uaccugacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu cgccggcgcc cuggaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu caagugccgc cugaagaugg acaagcugcg gcucaagggc      960

gucagcuacu cccugugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccuc accccggugg ggcgccugau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcucga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccuggggg acaccgccug ggacuucggc ucc                                  1353


<210>  1327
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE CDS GC

<400>  1327
agcaagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1328
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE CDS GC

<400>  1328
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cugguaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1329
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE CDS GC

<400>  1329
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

aagcugagcu ccgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcggcucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc ggggcgccaa gcgcauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1330
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE CDS GC

<400>  1330
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacac ccagacccug acgccggugg ggcgccucau caccgccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacgcacca cuggcaccgg     1260

uccgggagca ccaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1331
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE CDS GC

<400>  1331
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgaga uccgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

gugcacggcu cccagcacag cgggaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgcgaagcgc cagaccgugg ugguccuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gugagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ucacggugga gguccaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug accccggucg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1332
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE CDS GC

<400>  1332
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgcccg gcgcauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1333
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE CDS GC

<400>  1333
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggu cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgccaaguu cgcgugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca aggucgagau cacccccaac uccccgcgcg ccgaggccac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcuggg gagccaggag      840

ggcgcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg ggcgaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gugagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ucacggugga ggugcaguac gccggcaccg acggccccug caaggucccc     1080

gcgcagaugg ccguggacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgucauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgugau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gccaccgucc gcggcgcgaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1334
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE CDS GC

<400>  1334
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcau cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca ccaucggcaa ggccuucgag gcgacggugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1335
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE CDS GC

<400>  1335
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cgggaucggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1336
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE CDS GC

<400>  1336
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccuggccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcucg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcugg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ucguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac agcccccgcg ccgaggcgac gcucgggggc      600

uucggcuccc uggggcugga cugcgagccg cggaccggcc uggacuucuc cgaccucuac      660

uaccugacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggggc cgacaccggc acgccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu cgccggcgcc cuggaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu caagugccgc cugaagaugg acaagcugcg gcucaagggc      960

gucagcuacu cccugugcac cgccgccuuc acguucacca agaucccggc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgugg ggcgccugau cacggccaac     1140

ccggugauca ccgagagcac cgagaacucc aagaugaugc uggagcucga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccuggggg acaccgccug ggacuucggc ucc                                  1353


<210>  1337
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE CDS GC

<400>  1337
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gacccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagca gcgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucuccuaca gccucugcac ggccgccuuc accuucacca agauccccgc cgagacccug     1020

cacgggacgg ugaccgucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug acgccggugg ggcgccucau caccgccaac     1140

cccgugauca ccgaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacgcacca cuggcaccgg     1260

agcgggucca ccaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1338
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE CDS GC

<400>  1338
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugcug     1080

gcccagaugg ccgucgacau gcagacccuc acccccgugg ggcgccugau cacggccaac     1140

ccggugauca ccgagagcac cgagaacucc aagaugaugc uggagcucga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccuggggg acaccgccug ggacuucggc ucc                                  1353


<210>  1339
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE CDS GC

<400>  1339
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggaggccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1340
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE CDS GC

<400>  1340
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcggc ccucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggacgggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcg cucgaggccg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc accuucacga agauccccgc ggagacccug     1020

cacgggaccg ugaccgucga ggugcaguac gccggcacgg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau caccgccaac     1140

cccgugauca cggagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca ccaucggcaa ggcguucgag gccacggugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgcgug ggacuucggc ucc                                  1353


<210>  1341
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE CDS GC

<400>  1341
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucagcaccac cguguccaac auggccgagg ugcgcagcua cugcuacgag      240

gccaccaucu ccgacaucgc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgccg ugguccuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcg cucgaggccg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gugagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc ggagacccug     1020

cacgggaccg ucacggugga gguccaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug accccggucg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggcguucgag gccaccgugc gcggcgccaa gcggauggcc     1320

gugcucgggg acaccgcgug ggacuucggc ucc                                  1353


<210>  1342
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE CDS GC

<400>  1342
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucagca agauccccgc cgagacccug     1020

cacgggaccg ugaccgagga gguccaguac gcgggcacgg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug accccggucg ggcgccucau caccgccaac     1140

cccgugauca cggaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgg     1260

agcgggucca ccaucggcaa ggccuucgag gcgacggugc gcggcgccaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1343
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE CDS GC

<400>  1343
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga gcugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccuc accccggucg ggcgccugau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcucga cccgcccuuc     1200

ggcgacagcu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

gugcuggggg acaccgccug ggacuucggc ucc                                  1353


<210>  1344
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE CDS GC

<400>  1344
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggca ucagcaaccg ggacuucgug gaggggaugu ccggcgggac cuggguggac      120

gucgugcugg agcacggcgg gugcgugacc gucauggcgc aggacaagcc cacgguggac      180

aucgagcucg ugaccaccac cgucagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

gugcacggcu cccagcacag cgggaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcaucc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgcgaagcgc cagaccgugg ugguccuggg gucccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagca gcgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ucacggugga gguccaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug accccggucg ggcgccucau cacggccaac     1140

cccgugauca ccgaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggggucggc gagaagaaga ucacccacca cuggcaccgg     1260

agcgggucca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1345
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE CDS GC

<400>  1345
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggucaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gugagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ucacggugga ggugcaguac gccggcaccg acggccccug caaggucccc     1080

gcgcagaugg ccguggacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgucauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgugau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gccaccgucc gcggcgcgaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1346
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE CDS GC

<400>  1346
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcgccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau caccgccaac     1140

cccgugauca cggagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca ccaucggcaa ggcguucgag gccacggugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgcgug ggacuucggc ucc                                  1353


<210>  1347
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE CDS GC

<400>  1347
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acaccggggc cgacacgggc accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgcggugc acaccgcccu ggccggcgcc cucgaggccg agauggacgg ggcgaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac ggccgccuuc accuucacca agauccccgc cgagacccug     1020

cacgggacgg ugaccgucga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcgcagaugg ccgucgacau gcagacccug acgccggugg ggcgccucau caccgccaac     1140

cccgugauca ccgagggcac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggggacuccu acaucgucau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1260

agcggcucca ccaucggcaa ggccuucgag gccaccgugc gcggggcgaa gcggauggcc     1320

guccucggcg acaccgccug ggacuucggg agc                                  1353


<210>  1348
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE CDS GC

<400>  1348
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gcgauggccc aggacaagcc cacgguggac      180

aucgagcucg ugaccaccac cgucagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggcguaccug      300

gacaagcaga gcgacaccca guacgugugc aagcgcaccc uggucgaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

gugcacggcu cccagcacag cgggaugcug gucaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg cggaggccac gcucgggggc      600

uucggcagcc ucgggcugga cugcgagccc cggaccggcc uggacuucuc cgaccucuac      660

uaccugacca ugaacaacaa gcacuggcug gcccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggggc cgcgaccggc acgccgcacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcuggg gagccaggag      840

ggcgccgucc acaccgcgcu cgccggcgcc cuggaggccg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu caagugccgc cugaagaugg acaagcugcg gcucaagggc      960

gugagcuacu cccugugcac cgcggccuuc acguucacca agauccccgc cgagaccgug     1020

gacgggaccg ucacggugga gggccaguac ggcgggaccg acggccccug caaggucccc     1080

gcccagaugg ccguggacau gcagacccug accccggugg ggcgccucau cacggcgaac     1140

cccgucauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgugau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccggcagca cgaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcg     1320

gugcucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1349
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE CDS GC

<400>  1349
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cgccguggac      180

aucgagcucg ucacgaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cccagggcga ggcguaccug      300

gacaagcaga gcgacacgca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca cgggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg cggaggccac ccucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggacgggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcg cucgaggccg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc accuucacga agauccccgc ggagacccug     1020

cacgggaccg ugaccgucga ggugcaguac gccggcacgg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau caccgccaac     1140

cccgugauca cggagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca ccaucggcaa ggcguucgag gccacggugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgcgug ggacuucggc ucc                                  1353


<210>  1350
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE CDS GC

<400>  1350
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugaacaaccg ggacuucgug gaggggauga gcggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cguguccaac auggccgagg ugcgcagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cggugcccca cgcagggcga ggccuaccug      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggagc cuggugaccu gcgcgaaguu cgccugcucc      420

aagaagauga cgggcaagag cauccagccc gagaaccugg aguaccgcau caugcucucc      480

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac agcccgcgcg ccgaggcgac gcucgggggc      600

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gucccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagca gcgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucuccuaca gccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgugauca ccgaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

agcgggucca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1351
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE CDS GC

<400>  1351
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcgc cgccgccuuc accuucacga agauccccgc ggagacccug     1020

cacgggaccg ugaccgucga ggugcaguac gccggcacgg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau caccgccaac     1140

cccgugauca cggagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgg     1260

uccgggagca ccaucggcaa ggcguucgag gccacggugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgcgug ggacuucggc ucc                                  1353


<210>  1352
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE CDS GC

<400>  1352
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ucggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac ccucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggacgggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagacggugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc accuucacga agauccccgc cgagacccug     1020

cacgggaccg ugaccgucga ggugcaguac gcgggcacgg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau caccgccaac     1140

cccgugauca cggagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gacaagaaga ucacccacca cuggcaccgg     1260

uccgggagca ccaucggcaa ggccuucgag gcgacggugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1353
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE CDS GC

<400>  1353
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cgccugcagc      420

aagaagauga cgggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggagau cacccccaac uccccgcgcg ccgaggcgac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgc cagaccgugg ucgugcuggg gagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cggcugagcu ccgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

gucagcuacu cccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau cacggccaac     1140

cccgugauca ccgagagcac cgagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacagcu acaucgucau cggggugggc gacaagaaga ucacccacca cugguaccgg     1260

uccgggagca cgaucggcaa ggccuucgag gcgaccgugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc ucc                                  1353


<210>  1354
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE CDS GC

<400>  1354
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgggcca agguggaggu cacgcccaac uccccgcgcg ccgaggccac ccucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggggc cgacacgggc accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcuggg gagccaggag      840

ggcgcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg ggcgaagggc      900

cggcuguuca gcgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccucugcac ggccgccuuc accuucacca agguccccgc cgagacccug     1020

cacgggacgg ugaccgugga gguccaguac gccggcaccg acggccccug caaggugccc     1080

gcgcagaugg ccguggacau gcagacccug acgccggucg ggcgccucau caccgccaac     1140

cccgugauca ccgaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggggucggc gacaagaaga ucacgcacca cuggcaccgg     1260

agcgggucca ccaucggcaa ggccuucgag gccaccgugc gcggcgcgaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1355
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE CDS GC

<400>  1355
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ucggccacga gaccgacgag      540

aaccgggcca agguggaggu cacccccaac uccccgcgcg ccgaggccac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcuggg gagccaggag      840

ggcgcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg ggcgaagggc      900

cggcuguuca gcgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccucugcac cgccgccuuc acguucacca agguccccgc cgagacccug     1020

cacgggaccg ugacggugga gguccaguac gccggcaccg acggccccug caaggugccc     1080

gcgcagaugg ccguggacau gcagacccug accccggucg ggcgccucau cacggccaac     1140

cccgugauca ccgaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggggucggc gacaagaaga ucacccacca cuggcaccgg     1260

agcgggucca cgaucggcaa ggccuucgag gccaccgugc gcggcgcgaa gcggauggcc     1320

gugcucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1356
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE CDS GC

<400>  1356
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ucggccacga gaccgacgag      540

aaccgggcca agguggaggu cacccccaac uccccgcgcg ccgaggccac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cggaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagaccgucg uggugcuggg gagccaggag      840

ggcgcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg ggcgaagggc      900

cggcuguuca gcgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccucugcac cgccgucugc acggccgcca aggugcccgc cgagacccug     1020

cacgggaccg ugaccgucga ggugcaguac gcgggcacgg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcgccucau caccgccaac     1140

cccgugauca cggaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgucau cggggugggc gacaagaaga ucacccacca cuggcaccgg     1260

agcgggucca ccaucggcaa ggccuucgag gcgacggugc gcggcgccaa gcggauggcc     1320

guccucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1357
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE CDS GC

<400>  1357
agccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgc       60

ugcaucggcg ugagcaaccg ggacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgcuccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cggugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      360

aacggcugcg gccucuucgg gaaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccgcau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgacg agaaccgggc caagguggag      540

gucaccccca acuccccgcg cgccgaggcc acccugggcg gguucggcag ccugggccuc      600

gacugcgagc cccggacggg gcuggacuuc uccgaccugu acuaccucac caugaacaac      660

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacagcggc      720

gcggacaccg agaccccgca cuggaacaac aaggaggccc ucguggaguu caaggacgcc      780

cacgccaagc gccagacggu cguggugcug gggagccagg agggcgccgu ccacaccgcg      840

cuggccgggg cccucgaggc cgagauggac ggcgccaagg gccggcuguc cagcgggcac      900

cugaagugcc gccucaagau ggacaagcug cggcugaagg gcguguccua cagccucugc      960

accgcggccu ucaccuucac gaaggucccc gccgagaccc ugcacgggac cgugaccgug     1020

gagguccagu acgccggccg cgacgggccc ugcaaggugc ccgcccagau ggcgguggac     1080

augcagacgc ugaccccggu cggccggcuc aucaccgcca accccgugau caccgagucc     1140

acggagaaca gcaagaugau gcuggagcug gacccgcccu ucggcgacuc cuacaucgug     1200

aucggggucg gcgacaagaa gaucacccac cacuggcacc gcagcggguc caucaucggc     1260

aaggccuucg aggccaccgu gcgcggggcc aagcggaugg cggugcucgg cgacaccgcc     1320

ugggacuucg gcagc                                                      1335


<210>  1358
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE CDS GC

<400>  1358
agccagaagg ugaucuaccu ggucaugauc gugcucaucg ugcccgccua cuccaucagc       60

ugcaucggcg ucuccaaccg cgaccuggug gaggggauga gcggcgggac cuggguggac      120

gucgugcugg agcacggcgg gugcgugacc gagauggccc aggacaagcc cacggucgac      180

aucgagcucg ugaccaugac cguguccaac auggcggagg uccggagcua cugcuacgag      240

gccucccuga gcgacauggc cuccgccagc cgcugcccca cccagggcga gcccucccug      300

gacaagcaga gcgacacgca guccgugugc aagcggaccc ucggcgaccg cggguggggc      360

aacgggugcg gcaucuucgg gaagggcagc cuggugaccu gcuccaaguu cacgugcugc      420

aagaagaugc ccggcaagag cauccagccg gagaaccugg aguaccggau caugcucccc      480

guccacgggu cccagcacag cggcaugauc gugaacgaca ucgggcacga gaccgacgag      540

aaccgcgcca agguggaggu cacccccaac agcccccggg cggaggccac ccugggcggc      600

uucggguccc ugggccucga cugcgagccg cgcaccgggc uggacuucag cgaccuguac      660

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggg acgccccacu ggaacaacaa ggaggcgcuc      780

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcuggg cucccaggag      840

ggcgccgucc acaccgcccu ggcgggggcc cucgaggccg agauggacgg cgccaagggg      900

cgccuguuca gcggccaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggg      960

guguccuaca gccucugcac cgccgcguuc accuucacca aggucccggc cgagacgcug     1020

cacggcaccg ugaccgugga gguccagucc gccggcaccg acgggcccug caaggugccc     1080

gcccagaugg ccguggacau gcagacgcug acccccgucg gccggcucau caccgcgaac     1140

ccggugauca ccgagagcac ggagaacucc aagaugaugc uggagcugga cccgcccuuc     1200

ggggacagcu acaucgugau cggcgucggg gacaagaaga ucacccacca cuggcaccgc     1260

uccggcagca ccaucggcaa ggccuucgag gccaccgugc gcggggccaa gcggauggcc     1320

gugcucggcg acacggcgug ggacuucggg ucc                                  1353


<210>  1359
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE CDS GC

<400>  1359
cgccagaagg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cagcauccgg       60

ugcaucggcg uguccaaccg cgacuucgug gaggggauga gcggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cguguccaac auggccgagg ugcggagcua cugcuacgag      240

gccuccauca gcgacauggc cuccgacagc cgcugcccca cgcagggcga ggccuaccug      300

gacaagcagu ccgacaccca guacgucugc aagcggaccc ugguggaccg cggcuggggg      360

aacggcugcg ggcuguucgg caaggggagc cuggugaccu gcgcgaaguu cgccugcucc      420

aagaagauga cgggcaagag cauccagccc gagaaccugg aguaccggau caugcucucc      480

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gaccgacgag      540

aaccgcgcca agguggagau cacccccaac agcccgcggg ccgaggcgac gcucgggggc      600

uucggcuccc uggggcucga cugcgagccc cgcaccggcc uggacuucag cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgcgaagcgg cagaccgugg ucgugcuggg gucccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cucgaggcgg agauggacgg ggccaagggc      900

cgccugagca gcgggcaccu gaagugccgg cucaagaugg acaagcugcg ccugaagggc      960

gucuccuaca gccucugcac cgccgccuuc acguucacca agauccccgc cgagacccug     1020

cacgggaccg ugacggucga ggugcaguac gcgggcaccg acggccccug caaggugccc     1080

gcccagaugg ccgucgacau gcagacccug accccggugg ggcggcucau cacggccaac     1140

cccgugauca ccgaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgucau cggggugggc gagaagaaga ucacccacca cuggcaccgc     1260

agcgggucca cgaucggcaa ggccuucgag gcgaccgugc ggggcgccaa gcgcauggcc     1320

guccucgggg acaccgccug ggacuucggc agc                                  1353


<210>  1360
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE CDS GC

<400>  1360
agccagcgcg ugaucuaccu ggucaugauc cuccugaucg cccccgccua cuccauccgg       60

ugcaucggcg ugagcaaccg cgacuucgug gaggggaugu ccggcgggac cugggucgac      120

guggugcugg agcacggcgg gugcgucacc gugauggcgc aggacaagcc cacgguggac      180

aucgagcucg ucaccaccac cgugagcaac auggccgagg ugcgguccua cugcuacgag      240

gccagcaucu ccgacauggc cagcgacucc cgcugcccca cgcagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgucugc aagcggaccc ugguggaccg cggcuggggg      360

aacggcugcg ggcuguucgg caaggggucc cuggugaccu gcgcgaaguu cacgugcagc      420

aagaagauga ccggcaaguc cauccagccc gagaaccugg aguaccggau caugcucagc      480

guccacggcu cccagcacag cgggaugauc gugaacgaca ucggccacga gaccgacgag      540

aaccgcgcca agguggaggu cacccccaac uccccgcggg ccgaggccac gcucgggggc      600

uucggcagcc uggggcucga cugcgagccc cgcaccggcc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggggc cgacaccggc acgccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcuggg gagccaggag      840

ggcgcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg ggcgaagggc      900

cgccuguuca gcgggcaccu gaagugccgg aucaagaugg acaagcuccg ccugaagggc      960

guguccuaca gccugugcac cgccgccuuc acguucucca agguccccgc cgagacccuc     1020

cacgggaccg ugaccgugga gguccaguac gccggccggg acggccccug caaggugccc     1080

gcgcagaugg ccguggacau gcagacgcug accccggucg ggcgccugau caccgccaac     1140

cccaucauca ccgagagcac ggagaacucc cggaugaugc ucgagcugga cccgcccuuc     1200

ggcgacagcu acaucgugau cggggugggc gacaagaaga ucacccacca cuggcaccgc     1260

uccgggagca ccaucggcaa ggccuucgag gccaccgucc ggggcgcgaa gcgcauggcc     1320

gugcuggggg acacggccug ggacuucggc ucc                                  1353


<210>  1361
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  1361
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1362
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS opt

<400>  1362
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ccugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacgc caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1363
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS opt

<400>  1363
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag     1080

gucgagguca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gucuucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucgu cuggcucggc cucaacacca agaacggcuc caucucccuc     2040

accugccucg cccucggcgg cgucaugauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugcuccg ucgacuucuc caag                                            2124


<210>  1364
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) CDS opt

<400>  1364
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1365
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  1365
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1366
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) CDS opt

<400>  1366
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaccugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacgccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1367
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) CDS opt

<400>  1367
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucggcua cgagaccgac     1020

gaggaccgcg ccaaggucga ggucaccccc aacucccccc gcgccgaggc cacccucggc     1080

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1140

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1200

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1320

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1380

ggccgccucu ucuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1440

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagguccc cgccgagacc     1500

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagauc     1560

cccguccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1620

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1680

uucggcgacu ccuacaucgu caucggcguc ggcgacaaga agaucaccca ccacuggcac     1740

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1800

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgucuu caacucccuc     1860

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1920

ugguucuccc agauccucau cggcacccuc cucgucuggc ucggccucaa caccaagaac     1980

ggcuccaucu cccucaccug ccucgcccuc ggcggcguca ugaucuuccu cuccaccgcc     2040

gucuccgcc                                                             2049


<210>  1368
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) CDS opt

<400>  1368
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1369
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS opt

<400>  1369
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1370
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS opt

<400>  1370
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1371
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS opt

<400>  1371
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag      540

gucgagguca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc      600

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug      660

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac      720

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag      780

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac      840

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc      900

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc      960

cucugcaccg ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc     1020

accgucgagg uccaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcc     1080

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1140

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1200

aucgucaucg gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc     1260

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1320

accgccuggg acuucggcuc c                                               1341


<210>  1372
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) CDS opt

<400>  1372
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1373
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) CDS opt

<400>  1373
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uccggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1374
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) CDS opt

<400>  1374
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaccugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uccggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacgccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1375
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) CDS opt

<400>  1375
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uccggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucggcua cgagaccgac     1020

gaggaccgcg ccaaggucga ggucaccccc aacucccccc gcgccgaggc cacccucggc     1080

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1140

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1200

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1320

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1380

ggccgccucu ucuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1440

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagguccc cgccgagacc     1500

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagauc     1560

cccguccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1620

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1680

uucggcgacu ccuacaucgu caucggcguc ggcgacaaga agaucaccca ccacuggcac     1740

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1800

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgucuu caacucccuc     1860

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1920

ugguucuccc agauccucau cggcacccuc cucgucuggc ucggccucaa caccaagaac     1980

ggcuccaucu cccucaccug ccucgcccuc ggcggcguca ugaucuuccu cuccaccgcc     2040

gucuccgcc                                                             2049


<210>  1376
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) CDS opt

<400>  1376
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uccggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1377
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) CDS opt

<400>  1377
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucca ggacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1378
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) CDS opt

<400>  1378
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaccugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucca ggacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacgccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1379
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) CDS opt

<400>  1379
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucca ggacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1380
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1380
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccacccuc ggcaaggccu ucuccaccac ccucaagggc     1800

gcccagcgcc ucgccgcccu cggcgacacc gccugggacu ucggcuccau cggcggcguc     1860

uucaacucca ucggccgcgc cguccaccag gucuucggcg gcgccuuccg cacccucuuc     1920

ggcggcaugu ccuggaucac ccagggccuc augggcgccc uccuccucug gaugggcguc     1980

aacgcccgcg accgcuccau cgcccucgcc uuccucgcca ccggcggcgu ccucgucuuc     2040

cucgccacca acguccacgc c                                               2061


<210>  1381
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1381
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaccugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccacccuc ggcaaggccu ucuccaccac ccucaagggc     1800

gcccagcgcc ucgccgcccu cggcgacacc gccugggacu ucggcuccau cggcggcguc     1860

uucaacucca ucggccgcgc cguccaccag gucuucggcg gcgccuuccg cacccucuuc     1920

ggcggcaugu ccuggaucac ccagggccuc augggcgccc uccuccucug gaugggcguc     1980

aacgcccgcg accgcuccau cgcccucgcc uuccucgcca ccggcggcgu ccucgucuuc     2040

cucgccacca acguccacgc c                                               2061


<210>  1382
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       CDS opt

<400>  1382
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucggcua cgagaccgac     1020

gaggaccgcg ccaaggucga ggucaccccc aacucccccc gcgccgaggc cacccucggc     1080

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1140

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1200

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1320

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1380

ggccgccucu ucuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1440

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagguccc cgccgagacc     1500

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagauc     1560

cccguccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1620

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1680

uucggcgacu ccuacaucgu caucggcguc ggcgacaaga agaucaccca ccacuggcac     1740

cgcuccggcu ccacccucgg caaggccuuc uccaccaccc ucaagggcgc ccagcgccuc     1800

gccgcccucg gcgacaccgc cugggacuuc ggcuccaucg gcggcgucuu caacuccauc     1860

ggccgcgccg uccaccaggu cuucggcggc gccuuccgca cccucuucgg cggcaugucc     1920

uggaucaccc agggccucau gggcgcccuc cuccucugga ugggcgucaa cgcccgcgac     1980

cgcuccaucg cccucgccuu ccucgccacc ggcggcgucc ucgucuuccu cgccaccaac     2040

guccacgcc                                                             2049


<210>  1383
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1383
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccacccuc ggcaaggccu ucuccaccac ccucaagggc     1800

gcccagcgcc ucgccgcccu cggcgacacc gccugggacu ucggcuccau cggcggcguc     1860

uucaacucca ucggccgcgc cguccaccag gucuucggcg gcgccuuccg cacccucuuc     1920

ggcggcaugu ccuggaucac ccagggccuc augggcgccc uccuccucug gaugggcguc     1980

aacgcccgcg accgcuccau cgcccucgcc uuccucgcca ccggcggcgu ccucgucuuc     2040

cucgccacca acguccacgc c                                               2061


<210>  1384
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  1384
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1385
<211>  2133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1385
aucaucaacg cccgcaagga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgacgucg gcugcuccgu cgacuucucc aag                                  2133


<210>  1386
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1386
aucaacgccc gcaaggagaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gacgucggcu gcuccgucga cuucuccaag                                      2130


<210>  1387
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1387
aacgcccgca aggagaagaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugcu ccgucgacuu cuccaag                                         2127


<210>  1388
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1388
gcccgcaagg agaagaagcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugcuccg ucgacuucuc caag                                            2124


<210>  1389
<211>  2121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1389
cgcaaggaga agaagcgccg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcuccgucg acuucuccaa g                                               2121


<210>  1390
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1390
aaggagaaga agcgccgcgg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

uccgucgacu ucuccaag                                                   2118


<210>  1391
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1391
gagaagaagc gccgcggcgc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgacgu cggcugcucc     2100

gucgacuucu ccaag                                                      2115


<210>  1392
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1392
aagaagcgcc gcggcgccga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgacgucgg cugcuccguc     2100

gacuucucca ag                                                         2112


<210>  1393
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1393
aagcgccgcg gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cuccgucgac     2100

uucuccaag                                                             2109


<210>  1394
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1394
cgccgcggcg ccgacaccuc cgucggcauc gucggccucc uccucaccac cgccauggcc       60

gccgagguca cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc      120

gaggccaucu ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac      180

cucggccaca ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc      240

gagcccgacg acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc      300

ugccaccaca agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac      360

uccacccgca agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag      420

caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc      480

gccaucgccu ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc      540

cuccucaucg cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc      600

gagggcaugu ccggcggcac cugggucgac aucguccucg agcacggcgg cugcgucacc      660

gucauggccc aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac      720

auggccgagg uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc      780

cgcugcccca cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc      840

aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc      900

cucgucaccu gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc      960

gagaaccucg aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc     1020

gucaacgaca ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac     1080

uccccccgcg ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc     1140

cgcaccggcc ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc     1200

guccacaagg agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc     1320

cagaccgucg ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc     1380

cucgaggccg agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc     1440

cucaagaugg acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac     1560

gccggcaccg acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc     1620

acccccgucg gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc     1680

aagaugaugc ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc     1740

gagaagaaga ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc     1860

uccgucggcg gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaaguccc ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc     1980

auguggcucg gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc     2040

ggcguccuca ucuuccucuc caccgccguc uccgccgacg ucggcugcuc cgucgacuuc     2100

uccaag                                                                2106


<210>  1395
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1395
cgcggcgccg acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc       60

gaggucaccc gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag      120

gccaucuccu uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc      180

ggccacaugu gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag      240

cccgacgacg ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc      300

caccacaaga agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc      360

acccgcaagc uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac      420

cucauccgcg ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc      480

aucgccuggc uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc      540

cucaucgccc ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag      600

ggcauguccg gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc      660

auggcccagg acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug      720

gccgaggucc gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc      780

ugccccaccc agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag      840

cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc      900

gucaccugcg ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag      960

aaccucgagu accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc     1020

aacgacaccg gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc     2040

guccucaucu uccucuccac cgccgucucc gccgacgucg gcugcuccgu cgacuucucc     2100

aag                                                                   2103


<210>  1396
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1396
ggcgccgaca ccuccgucgg caucgucggc cuccuccuca ccaccgccau ggccgccgag       60

gucacccgcc gcggcuccgc cuacuacaug uaccucgacc gcaacgacgc cggcgaggcc      120

aucuccuucc ccaccacccu cggcaugaac aagugcuaca uccagaucau ggaccucggc      180

cacaugugcg acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc      240

gacgacgucg acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc      360

cgcaagcucc agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc      420

auccgcgucg agaacuggau cuuccgcaac cccggcuucg cccucgccgc cgccgccauc      480

gccuggcucc ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc      540

aucgcccccg ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc      600

auguccggcg gcaccugggu cgacaucguc cucgagcacg gcggcugcgu caccgucaug      660

gcccaggaca agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc      720

gagguccgcu ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc      780

cccacccagg gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc      840

acccucgucg accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc      900

accugcgcca aguucgccug cuccaagaag augaccggca aguccaucca gcccgagaac      960

cucgaguacc gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac     1020

gacaccggcc acgagaccga cgagaaccgc gccaaggucg agaucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

accgacggcc ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgagaag     1740

aagaucaccc accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc     1800

guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc     1860

ggcggcgccc ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

ucccucuucg gcggcauguc cugguucucc cagauccuca ucggcacccu ccucaugugg     1980

cucggccuca acaccaagaa cggcuccauc ucccucaugu gccucgcccu cggcggcguc     2040

cucaucuucc ucuccaccgc cgucuccgcc gacgucggcu gcuccgucga cuucuccaag     2100


<210>  1397
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1397
gccgacaccu ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc       60

acccgccgcg gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc      120

uccuucccca ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac      180

augugcgacg ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac      240

gacgucgacu gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac      300

aagaagggcg aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc      360

aagcuccaga cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc      420

cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc      480

uggcuccucg gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc      540

gcccccgccu acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug      600

uccggcggca ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc      660

caggacaagc ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag      720

guccgcuccu acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc      780

acccagggcg aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc      840

cucgucgacc gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc      900

ugcgccaagu ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc      960

gaguaccgca ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac     1020

accggccacg agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagauccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgcccuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc     2040

aucuuccucu ccaccgccgu cuccgccgac gucggcugcu ccgucgacuu cuccaag        2097


<210>  1398
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1398
gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1800

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1860

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1920

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1980

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2040

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caag           2094


<210>  1399
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1399
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc cgacgucggc ugcuccgucg acuucuccaa g              2091


<210>  1400
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1400
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccgc cgucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc cgacgucggc ugcuccgucg acuucuccaa g              2091


<210>  1401
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1401
cgcggcgccg acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc       60

gccgucaccc gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag      120

gccaucuccu uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc      180

ggccacaugu gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag      240

cccgacgacg ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc      300

caccacaaga agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc      360

acccgcaagc uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac      420

cucauccgcg ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc      480

aucgccuggc uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc      540

cucaucgccc ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag      600

ggcauguccg gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc      660

auggcccagg acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug      720

gccgaggucc gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc      780

ugccccaccc agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag      840

cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc      900

gucaccugcg ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag      960

aaccucgagu accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc     1020

aacgacaccg gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc     2040

guccucaucu uccucuccac cgccgucucc gccgacgucg gcugcuccgu cgacuucucc     2100

aag                                                                   2103


<210>  1402
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1402
cgcggccucu ccucccgcaa gcgccgcucc cacgacgucc ucaccgucca guuccucauc       60

cucggcaugc uccucaugac cggcggcguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugcu ccgucgacuu cuccaag                                         2127


<210>  1403
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1403
cgcggccucu ccucccgcaa gcgccgcucc cacgacgucc ucaccgucca guuccucauc       60

cucggcaugc uccucaugac cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugcu ccgucgacuu cuccaag                                         2127


<210>  1404
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1404
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugc                                                   2118


<210>  1405
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1405
aucaucaacg cccgcaagga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgacgucg gcugc                                                      2115


<210>  1406
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1406
aucaacgccc gcaaggagaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gacgucggcu gc                                                         2112


<210>  1407
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1407
aacgcccgca aggagaagaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugc                                                             2109


<210>  1408
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1408
gcccgcaagg agaagaagcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugc                                                                2106


<210>  1409
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1409
cgcaaggaga agaagcgccg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugc                                                                   2103


<210>  1410
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1410
aaggagaaga agcgccgcgg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100


<210>  1411
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1411
gagaagaagc gccgcggcgc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgacgu cggcugc        2097


<210>  1412
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1412
aagaagcgcc gcggcgccga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgacgucgg cugc           2094


<210>  1413
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1413
aagcgccgcg gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug c              2091


<210>  1414
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1414
cgccgcggcg ccgacaccuc cgucggcauc gucggccucc uccucaccac cgccauggcc       60

gccgagguca cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc      120

gaggccaucu ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac      180

cucggccaca ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc      240

gagcccgacg acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc      300

ugccaccaca agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac      360

uccacccgca agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag      420

caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc      480

gccaucgccu ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc      540

cuccucaucg cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc      600

gagggcaugu ccggcggcac cugggucgac aucguccucg agcacggcgg cugcgucacc      660

gucauggccc aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac      720

auggccgagg uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc      780

cgcugcccca cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc      840

aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc      900

cucgucaccu gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc      960

gagaaccucg aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc     1020

gucaacgaca ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac     1080

uccccccgcg ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc     1140

cgcaccggcc ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc     1200

guccacaagg agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc     1320

cagaccgucg ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc     1380

cucgaggccg agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc     1440

cucaagaugg acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac     1560

gccggcaccg acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc     1620

acccccgucg gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc     1680

aagaugaugc ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc     1740

gagaagaaga ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc     1860

uccgucggcg gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaaguccc ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc     1980

auguggcucg gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc     2040

ggcguccuca ucuuccucuc caccgccguc uccgccgacg ucggcugc                  2088


<210>  1415
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1415
cgcggcgccg acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc       60

gaggucaccc gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag      120

gccaucuccu uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc      180

ggccacaugu gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag      240

cccgacgacg ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc      300

caccacaaga agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc      360

acccgcaagc uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac      420

cucauccgcg ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc      480

aucgccuggc uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc      540

cucaucgccc ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag      600

ggcauguccg gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc      660

auggcccagg acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug      720

gccgaggucc gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc      780

ugccccaccc agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag      840

cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc      900

gucaccugcg ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag      960

aaccucgagu accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc     1020

aacgacaccg gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc     2040

guccucaucu uccucuccac cgccgucucc gccgacgucg gcugc                     2085


<210>  1416
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1416
ggcgccgaca ccuccgucgg caucgucggc cuccuccuca ccaccgccau ggccgccgag       60

gucacccgcc gcggcuccgc cuacuacaug uaccucgacc gcaacgacgc cggcgaggcc      120

aucuccuucc ccaccacccu cggcaugaac aagugcuaca uccagaucau ggaccucggc      180

cacaugugcg acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc      240

gacgacgucg acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc      360

cgcaagcucc agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc      420

auccgcgucg agaacuggau cuuccgcaac cccggcuucg cccucgccgc cgccgccauc      480

gccuggcucc ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc      540

aucgcccccg ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc      600

auguccggcg gcaccugggu cgacaucguc cucgagcacg gcggcugcgu caccgucaug      660

gcccaggaca agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc      720

gagguccgcu ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc      780

cccacccagg gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc      840

acccucgucg accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc      900

accugcgcca aguucgccug cuccaagaag augaccggca aguccaucca gcccgagaac      960

cucgaguacc gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac     1020

gacaccggcc acgagaccga cgagaaccgc gccaaggucg agaucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

accgacggcc ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgagaag     1740

aagaucaccc accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc     1800

guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc     1860

ggcggcgccc ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

ucccucuucg gcggcauguc cugguucucc cagauccuca ucggcacccu ccucaugugg     1980

cucggccuca acaccaagaa cggcuccauc ucccucaugu gccucgcccu cggcggcguc     2040

cucaucuucc ucuccaccgc cgucuccgcc gacgucggcu gc                        2082


<210>  1417
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1417
gccgacaccu ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc       60

acccgccgcg gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc      120

uccuucccca ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac      180

augugcgacg ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac      240

gacgucgacu gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac      300

aagaagggcg aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc      360

aagcuccaga cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc      420

cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc      480

uggcuccucg gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc      540

gcccccgccu acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug      600

uccggcggca ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc      660

caggacaagc ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag      720

guccgcuccu acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc      780

acccagggcg aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc      840

cucgucgacc gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc      900

ugcgccaagu ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc      960

gaguaccgca ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac     1020

accggccacg agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagauccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgcccuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc     2040

aucuuccucu ccaccgccgu cuccgccgac gucggcugc                            2079


<210>  1418
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1418
gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1800

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1860

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1920

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1980

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2040

uuccucucca ccgccgucuc cgccgacguc ggcugc                               2076


<210>  1419
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1419
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc cgacgucggc ugc                                  2073


<210>  1420
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1420
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccgc cgucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc cgacgucggc ugc                                  2073


<210>  1421
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1421
cgcggcgccg acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc       60

gccgucaccc gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag      120

gccaucuccu uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc      180

ggccacaugu gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag      240

cccgacgacg ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc      300

caccacaaga agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc      360

acccgcaagc uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac      420

cucauccgcg ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc      480

aucgccuggc uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc      540

cucaucgccc ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag      600

ggcauguccg gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc      660

auggcccagg acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug      720

gccgaggucc gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc      780

ugccccaccc agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag      840

cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc      900

gucaccugcg ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag      960

aaccucgagu accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc     1020

aacgacaccg gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc     2040

guccucaucu uccucuccac cgccgucucc gccgacgucg gcugc                     2085


<210>  1422
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1422
cgcggccucu ccucccgcaa gcgccgcucc cacgacgucc ucaccgucca guuccucauc       60

cucggcaugc uccucaugac cggcggcguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugc                                                             2109


<210>  1423
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1423
cgcggccucu ccucccgcaa gcgccgcucc cacgacgucc ucaccgucca guuccucauc       60

cucggcaugc uccucaugac cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugc                                                             2109


<210>  1424
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1424
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgac                                                             2109


<210>  1425
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1425
aucaucaacg cccgcaagga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgac                                                                2106


<210>  1426
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1426
aucaacgccc gcaaggagaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gac                                                                   2103


<210>  1427
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1427
aacgcccgca aggagaagaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100


<210>  1428
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1428
gcccgcaagg agaagaagcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgac        2097


<210>  1429
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1429
cgcaaggaga agaagcgccg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgac           2094


<210>  1430
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1430
aaggagaaga agcgccgcgg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga c              2091


<210>  1431
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1431
gagaagaagc gccgcggcgc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgac                  2088


<210>  1432
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1432
aagaagcgcc gcggcgccga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgac                     2085


<210>  1433
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1433
aagcgccgcg gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg ac                        2082


<210>  1434
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1434
cgccgcggcg ccgacaccuc cgucggcauc gucggccucc uccucaccac cgccauggcc       60

gccgagguca cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc      120

gaggccaucu ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac      180

cucggccaca ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc      240

gagcccgacg acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc      300

ugccaccaca agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac      360

uccacccgca agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag      420

caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc      480

gccaucgccu ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc      540

cuccucaucg cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc      600

gagggcaugu ccggcggcac cugggucgac aucguccucg agcacggcgg cugcgucacc      660

gucauggccc aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac      720

auggccgagg uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc      780

cgcugcccca cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc      840

aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc      900

cucgucaccu gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc      960

gagaaccucg aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc     1020

gucaacgaca ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac     1080

uccccccgcg ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc     1140

cgcaccggcc ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc     1200

guccacaagg agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc     1320

cagaccgucg ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc     1380

cucgaggccg agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc     1440

cucaagaugg acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac     1560

gccggcaccg acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc     1620

acccccgucg gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc     1680

aagaugaugc ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc     1740

gagaagaaga ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc     1860

uccgucggcg gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaaguccc ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc     1980

auguggcucg gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc     2040

ggcguccuca ucuuccucuc caccgccguc uccgccgac                            2079


<210>  1435
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1435
cgcggcgccg acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc       60

gaggucaccc gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag      120

gccaucuccu uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc      180

ggccacaugu gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag      240

cccgacgacg ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc      300

caccacaaga agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc      360

acccgcaagc uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac      420

cucauccgcg ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc      480

aucgccuggc uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc      540

cucaucgccc ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag      600

ggcauguccg gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc      660

auggcccagg acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug      720

gccgaggucc gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc      780

ugccccaccc agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag      840

cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc      900

gucaccugcg ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag      960

aaccucgagu accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc     1020

aacgacaccg gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc     2040

guccucaucu uccucuccac cgccgucucc gccgac                               2076


<210>  1436
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1436
ggcgccgaca ccuccgucgg caucgucggc cuccuccuca ccaccgccau ggccgccgag       60

gucacccgcc gcggcuccgc cuacuacaug uaccucgacc gcaacgacgc cggcgaggcc      120

aucuccuucc ccaccacccu cggcaugaac aagugcuaca uccagaucau ggaccucggc      180

cacaugugcg acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc      240

gacgacgucg acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc      360

cgcaagcucc agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc      420

auccgcgucg agaacuggau cuuccgcaac cccggcuucg cccucgccgc cgccgccauc      480

gccuggcucc ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc      540

aucgcccccg ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc      600

auguccggcg gcaccugggu cgacaucguc cucgagcacg gcggcugcgu caccgucaug      660

gcccaggaca agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc      720

gagguccgcu ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc      780

cccacccagg gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc      840

acccucgucg accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc      900

accugcgcca aguucgccug cuccaagaag augaccggca aguccaucca gcccgagaac      960

cucgaguacc gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac     1020

gacaccggcc acgagaccga cgagaaccgc gccaaggucg agaucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

accgacggcc ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgagaag     1740

aagaucaccc accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc     1800

guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc     1860

ggcggcgccc ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

ucccucuucg gcggcauguc cugguucucc cagauccuca ucggcacccu ccucaugugg     1980

cucggccuca acaccaagaa cggcuccauc ucccucaugu gccucgcccu cggcggcguc     2040

cucaucuucc ucuccaccgc cgucuccgcc gac                                  2073


<210>  1437
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1437
gccgacaccu ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc       60

acccgccgcg gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc      120

uccuucccca ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac      180

augugcgacg ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac      240

gacgucgacu gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac      300

aagaagggcg aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc      360

aagcuccaga cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc      420

cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc      480

uggcuccucg gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc      540

gcccccgccu acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug      600

uccggcggca ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc      660

caggacaagc ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag      720

guccgcuccu acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc      780

acccagggcg aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc      840

cucgucgacc gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc      900

ugcgccaagu ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc      960

gaguaccgca ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac     1020

accggccacg agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagauccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgcccuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc     2040

aucuuccucu ccaccgccgu cuccgccgac                                      2070


<210>  1438
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1438
gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1800

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1860

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1920

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1980

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2040

uuccucucca ccgccgucuc cgccgac                                         2067


<210>  1439
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1439
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc cgac                                            2064


<210>  1440
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1440
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccgc cgucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc cgac                                            2064


<210>  1441
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1441
cgcggcgccg acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc       60

gccgucaccc gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag      120

gccaucuccu uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc      180

ggccacaugu gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag      240

cccgacgacg ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc      300

caccacaaga agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc      360

acccgcaagc uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac      420

cucauccgcg ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc      480

aucgccuggc uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc      540

cucaucgccc ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag      600

ggcauguccg gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc      660

auggcccagg acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug      720

gccgaggucc gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc      780

ugccccaccc agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag      840

cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc      900

gucaccugcg ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag      960

aaccucgagu accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc     1020

aacgacaccg gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc     2040

guccucaucu uccucuccac cgccgucucc gccgac                               2076


<210>  1442
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1442
cgcggccucu ccucccgcaa gcgccgcucc cacgacgucc ucaccgucca guuccucauc       60

cucggcaugc uccucaugac cggcggcguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100


<210>  1443
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1443
cgcggccucu ccucccgcaa gcgccgcucc cacgacgucc ucaccgucca guuccucauc       60

cucggcaugc uccucaugac cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100


<210>  1444
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1444
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgcc                                                                2106


<210>  1445
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1445
aucaacgccc gcaaggagaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100


<210>  1446
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1446
aucaucaacg cccgcaagga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gcc                                                                   2103


<210>  1447
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1447
aacgcccgca aggagaagaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgcc        2097


<210>  1448
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1448
gcccgcaagg agaagaagcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgcc           2094


<210>  1449
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1449
cgcaaggaga agaagcgccg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc c              2091


<210>  1450
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1450
aaggagaaga agcgccgcgg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgcc                  2088


<210>  1451
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1451
gagaagaagc gccgcggcgc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgcc                     2085


<210>  1452
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1452
aagaagcgcc gcggcgccga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg cc                        2082


<210>  1453
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1453
aagcgccgcg gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgcc                            2079


<210>  1454
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1454
cgccgcggcg ccgacaccuc cgucggcauc gucggccucc uccucaccac cgccauggcc       60

gccgagguca cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc      120

gaggccaucu ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac      180

cucggccaca ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc      240

gagcccgacg acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc      300

ugccaccaca agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac      360

uccacccgca agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag      420

caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc      480

gccaucgccu ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc      540

cuccucaucg cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc      600

gagggcaugu ccggcggcac cugggucgac aucguccucg agcacggcgg cugcgucacc      660

gucauggccc aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac      720

auggccgagg uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc      780

cgcugcccca cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc      840

aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc      900

cucgucaccu gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc      960

gagaaccucg aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc     1020

gucaacgaca ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac     1080

uccccccgcg ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc     1140

cgcaccggcc ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc     1200

guccacaagg agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc     1320

cagaccgucg ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc     1380

cucgaggccg agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc     1440

cucaagaugg acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac     1560

gccggcaccg acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc     1620

acccccgucg gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc     1680

aagaugaugc ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc     1740

gagaagaaga ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc     1860

uccgucggcg gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaaguccc ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc     1980

auguggcucg gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc     2040

ggcguccuca ucuuccucuc caccgccguc uccgcc                               2076


<210>  1455
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1455
cgcggcgccg acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc       60

gaggucaccc gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag      120

gccaucuccu uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc      180

ggccacaugu gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag      240

cccgacgacg ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc      300

caccacaaga agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc      360

acccgcaagc uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac      420

cucauccgcg ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc      480

aucgccuggc uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc      540

cucaucgccc ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag      600

ggcauguccg gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc      660

auggcccagg acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug      720

gccgaggucc gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc      780

ugccccaccc agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag      840

cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc      900

gucaccugcg ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag      960

aaccucgagu accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc     1020

aacgacaccg gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc     2040

guccucaucu uccucuccac cgccgucucc gcc                                  2073


<210>  1456
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1456
ggcgccgaca ccuccgucgg caucgucggc cuccuccuca ccaccgccau ggccgccgag       60

gucacccgcc gcggcuccgc cuacuacaug uaccucgacc gcaacgacgc cggcgaggcc      120

aucuccuucc ccaccacccu cggcaugaac aagugcuaca uccagaucau ggaccucggc      180

cacaugugcg acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc      240

gacgacgucg acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc      360

cgcaagcucc agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc      420

auccgcgucg agaacuggau cuuccgcaac cccggcuucg cccucgccgc cgccgccauc      480

gccuggcucc ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc      540

aucgcccccg ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc      600

auguccggcg gcaccugggu cgacaucguc cucgagcacg gcggcugcgu caccgucaug      660

gcccaggaca agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc      720

gagguccgcu ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc      780

cccacccagg gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc      840

acccucgucg accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc      900

accugcgcca aguucgccug cuccaagaag augaccggca aguccaucca gcccgagaac      960

cucgaguacc gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac     1020

gacaccggcc acgagaccga cgagaaccgc gccaaggucg agaucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

accgacggcc ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgagaag     1740

aagaucaccc accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc     1800

guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc     1860

ggcggcgccc ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

ucccucuucg gcggcauguc cugguucucc cagauccuca ucggcacccu ccucaugugg     1980

cucggccuca acaccaagaa cggcuccauc ucccucaugu gccucgcccu cggcggcguc     2040

cucaucuucc ucuccaccgc cgucuccgcc                                      2070


<210>  1457
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1457
gccgacaccu ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc       60

acccgccgcg gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc      120

uccuucccca ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac      180

augugcgacg ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac      240

gacgucgacu gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac      300

aagaagggcg aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc      360

aagcuccaga cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc      420

cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc      480

uggcuccucg gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc      540

gcccccgccu acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug      600

uccggcggca ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc      660

caggacaagc ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag      720

guccgcuccu acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc      780

acccagggcg aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc      840

cucgucgacc gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc      900

ugcgccaagu ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc      960

gaguaccgca ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac     1020

accggccacg agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagauccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgcccuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc     2040

aucuuccucu ccaccgccgu cuccgcc                                         2067


<210>  1458
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1458
gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1800

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1860

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1920

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1980

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2040

uuccucucca ccgccgucuc cgcc                                            2064


<210>  1459
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  1459
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1460
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1460
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccgc cgucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1461
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1461
cgcggcgccg acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc       60

gccgucaccc gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag      120

gccaucuccu uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc      180

ggccacaugu gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag      240

cccgacgacg ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc      300

caccacaaga agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc      360

acccgcaagc uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac      420

cucauccgcg ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc      480

aucgccuggc uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc      540

cucaucgccc ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag      600

ggcauguccg gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc      660

auggcccagg acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug      720

gccgaggucc gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc      780

ugccccaccc agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag      840

cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc      900

gucaccugcg ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag      960

aaccucgagu accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc     1020

aacgacaccg gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc     2040

guccucaucu uccucuccac cgccgucucc gcc                                  2073


<210>  1462
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1462
cgcggccucu ccucccgcaa gcgccgcucc cacgacgucc ucaccgucca guuccucauc       60

cucggcaugc uccucaugac cggcggcguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgcc        2097


<210>  1463
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1463
cgcggccucu ccucccgcaa gcgccgcucc cacgacgucc ucaccgucca guuccucauc       60

cucggcaugc uccucaugac cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgcc        2097


<210>  1464
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long CDS opt

<400>  1464
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1465
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long CDS opt

<400>  1465
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ccugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacgc caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1466
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long CDS opt

<400>  1466
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag     1080

gucgagguca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gucuucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucgu cuggcucggc cucaacacca agaacggcuc caucucccuc     2040

accugccucg cccucggcgg cgucaugauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugcuccg ucgacuucuc caag                                            2124


<210>  1467
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long CDS opt

<400>  1467
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1468
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long CDS opt

<400>  1468
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggcuacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

aagcucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caagaucccc     1620

guccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1469
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long CDS opt

<400>  1469
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucacccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgacg agaaccgcgc caaggucgag     1080

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgucuuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucgucuggcu cggccucaac accaagaacg gcuccaucuc ccucaccugc     2040

cucgcccucg gcggcgucau gaucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

uccgucgacu ucuccaag                                                   2118


<210>  1470
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long CDS opt

<400>  1470
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccgca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggcuacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

aagcucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caagaucccc     1620

guccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1471
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long CDS opt

<400>  1471
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugacc gucaacgaca ucggcuacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

aagcucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caagaucccc     1620

guccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1472
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long CDS opt

<400>  1472
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc ggcuacgaga ccgacgagaa ccgcgccaag     1080

gucgagguca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggcaa gcucuucucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gucuucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucgu cuggcucggc cucaacacca agaacggcuc caucucccuc     2040

accugccucg cccucggcgg cgucaugauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugcuccg ucgacuucuc caag                                            2124


<210>  1473
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long CDS opt

<400>  1473
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

cgcggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1474
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long CDS opt

<400>  1474
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacgccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1475
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long CDS opt

<400>  1475
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gccucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1476
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long CDS opt

<400>  1476
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cugguaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1477
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long CDS opt

<400>  1477
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgaga uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1478
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long CDS opt

<400>  1478
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caagguccuc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1479
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long CDS opt

<400>  1479
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccacccucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1480
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long CDS opt

<400>  1480
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgucguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1481
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long CDS opt

<400>  1481
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccuccg ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcccccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1482
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long CDS opt

<400>  1482
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

uccaagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1483
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long CDS opt

<400>  1483
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

cgccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1484
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long CDS opt

<400>  1484
cgcaucauca acgcccgcaa ggagaagaag cgccgcuccg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacac ccagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1485
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long CDS opt

<400>  1485
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccaa cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgcccg ccgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1486
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long CDS opt

<400>  1486
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgagaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1487
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long CDS opt

<400>  1487
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cucccuccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1488
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long CDS opt

<400>  1488
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccaa cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggauguuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1489
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long CDS opt

<400>  1489
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggauccuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcaucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1490
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long CDS opt

<400>  1490
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucuccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccaccaucu ccgacaucgc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgccg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1491
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long CDS opt

<400>  1491
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cacccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1492
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long CDS opt

<400>  1492
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gcuccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1493
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long CDS opt

<400>  1493
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgagaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcaucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1494
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long CDS opt

<400>  1494
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccau cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1495
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long CDS opt

<400>  1495
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgucaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccu ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1496
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long CDS opt

<400>  1496
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

aagcucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1497
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long CDS opt

<400>  1497
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggaggccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1498
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long CDS opt

<400>  1498
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcgccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1499
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long CDS opt

<400>  1499
cgcaucauca acgccggcaa ggagaagaag cgccgcggcg ccgacaccaa cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgcccg ccgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1500
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long CDS opt

<400>  1500
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgagaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa ggacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1501
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long CDS opt

<400>  1501
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggu cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1502
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long CDS opt

<400>  1502
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccaa cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

augaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1503
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long CDS opt

<400>  1503
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccgc ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga cccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1504
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long CDS opt

<400>  1504
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgagaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccucgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1505
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long CDS opt

<400>  1505
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggca ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcaucc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1506
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long CDS opt

<400>  1506
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ccugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccauccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacgc caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1507
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long CDS opt

<400>  1507
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccagcgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1508
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long CDS opt

<400>  1508
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccaggc ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1509
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long CDS opt

<400>  1509
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1510
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long CDS opt

<400>  1510
cgcaucauca acgcccgcaa ggagaagaag cgccgcggca ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acaccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgagggcac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcguccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1511
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long CDS opt

<400>  1511
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gccauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugcuc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc gcccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgccaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagaccguc     1560

gacggcaccg ucaccgucga gggccaguac ggcggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucau cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccggcggcg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1512
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long CDS opt

<400>  1512
cgcaucauca acgcccgcaa ggagaagaag cgccgcggca ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucucca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgagga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1513
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long CDS opt

<400>  1513
cgcaucauca acgcccgcaa ggagaagaag cgccgcggca ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1514
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long CDS opt

<400>  1514
cgcaucauca acgcccgcaa ggagaagaag cgccgcggca ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gucgagguca cccgccgcgg caacgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac caccaugggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1515
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long CDS opt

<400>  1515
cgcaucauca acgcccgcaa ggagaagaag cgccgcggca ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucgucugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1516
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long CDS opt

<400>  1516
cgcaucauca acgcccgcaa ggagaagaag cgccgcggca ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gucgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc cgccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1517
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long CDS opt

<400>  1517
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gucgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gucaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1518
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long CDS opt

<400>  1518
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gucgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

gucaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cugguaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1519
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long CDS opt

<400>  1519
cgcaucauca acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gucgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuuccccac cacccucggc      180

gucaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1520
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long CDS opt

<400>  1520
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucacccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1521
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long CDS opt

<400>  1521
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac caaccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcgucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1522
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long CDS opt

<400>  1522
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1523
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long CDS opt

<400>  1523
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac caaccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgacg agaaccgcgc caaggucgag     1080

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgucuuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucgucuggcu cggccucaac accaagaacg gcuccaucuc ccucaccugc     2040

cucgcccucg gcggcgucau gaucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

uccgucgacu ucuccaag                                                   2118


<210>  1524
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long CDS opt

<400>  1524
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1525
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long CDS opt

<400>  1525
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccagcaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgucugc accgccgcca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1526
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long CDS opt

<400>  1526
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacgccuc caucggcauc       60

gucucccucc uccucaccac cgucauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgucac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgacg agaaccgcgc caaggucgag     1080

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacuccggc     1260

gccgacaccg agacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggccg cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caucaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgucuuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucgucuggcu cggccucaac accaagaacg gcuccaucuc ccucaccugc     2040

cucgcccucg gcggcgucau gaucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

uccgucgacu ucuccaag                                                   2118


<210>  1527
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long CDS opt

<400>  1527
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgacg agaaccgcgc caaggucgag     1080

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgucuuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucgucuggcu cggccucaac accaagaacg gcuccaucuc ccucaccugc     2040

cucgcccucg gcggcgucau gaucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

uccgucgacu ucuccaag                                                   2118


<210>  1528
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long CDS opt

<400>  1528
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac cacccucggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga gacccgccgc      360

ucccgccgcu ccgucucccu ccgcuaccac uacacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaagaag caccucauca uggucgagaa cuggaucuuc      480

cgcaaccccg gcuucgccau cgucuccguc gccaucaccu ggcucauggg cucccucacc      540

ucccagaagg ucaucuaccu cgucaugauc guccucaucg uccccgccua cuccaucucc      600

ugcaucggcg ucuccaaccg cgaccucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gagauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaugac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccucccucu ccgacauggc cuccgccucc cgcugcccca cccagggcga gcccucccuc      840

gacaagcagu ccgacaccca guccgucugc aagcgcaccc ucggcgaccg cggcuggggc      900

aacggcugcg gcaucuucgg caagggcucc cucgucaccu gcuccaaguu caccugcugc      960

aagaagaugc ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucccc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccagucc gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaag                               2136


<210>  1529
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long CDS opt

<400>  1529
cgcaucauca acgcccgcaa ggagcgcaag cgccgcggcg ccgacaccuc caucggcauc       60

aucggccucc uccucaccac cgccauggcc gccgagauca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcuc cgacgccggc aaggccaucu ccuucgccac caccaugggc      180

gucaacaagu gccacgucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgcccc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggauccuc      480

cgcaaccccg gcuucaccgu cgccgcccuc gccauggccu ggcuccucgg caacuccacc      540

ucccagcgcg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc aucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucucca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggccgcg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccaucauca ccgaguccac cgagaacucc cgcaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcgucc accaggucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucgccugccu cgcccucggc ggcgccauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc                                      2130


<210>  1530
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short CDS opt

<400>  1530
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1531
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short CDS opt

<400>  1531
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaccugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacgccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1532
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short CDS opt

<400>  1532
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucggcua cgagaccgac     1020

gaggaccgcg ccaaggucga ggucaccccc aacucccccc gcgccgaggc cacccucggc     1080

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1140

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1200

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1320

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1380

ggccgccucu ucuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1440

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagguccc cgccgagacc     1500

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagauc     1560

cccguccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1620

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1680

uucggcgacu ccuacaucgu caucggcguc ggcgacaaga agaucaccca ccacuggcac     1740

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1800

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgucuu caacucccuc     1860

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1920

ugguucuccc agauccucau cggcacccuc cucgucuggc ucggccucaa caccaagaac     1980

ggcuccaucu cccucaccug ccucgcccuc ggcggcguca ugaucuuccu cuccaccgcc     2040

gucuccgcc                                                             2049


<210>  1533
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short CDS opt

<400>  1533
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1534
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short CDS opt

<400>  1534
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

uacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggcaagcu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaaga uccccgucca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1535
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short CDS opt

<400>  1535
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccgcaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

uacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggcaagcu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaaga uccccgucca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1536
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short CDS opt

<400>  1536
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaccgucaa cgacaucggc     1020

uacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggcaagcu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaaga uccccgucca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1537
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short CDS opt

<400>  1537
accuccaucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc acccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacgagaac     1020

cgcgccaagg ucgaggucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1080

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1140

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1200

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1260

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1320

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1380

cucuucuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1440

uccuacuccc ucugcaccgc cgccuucacc uucaccaagg uccccgccga gacccuccac     1500

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1560

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1620

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1680

gacuccuaca ucgucaucgg cgucggcgac aagaagauca cccaccacug gcaccgcucc     1740

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1800

cucggcgaca ccgccuggga cuucggcucc gucggcggcg ucuucaacuc ccucggcaag     1860

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1920

ucccagaucc ucaucggcac ccuccucguc uggcucggcc ucaacaccaa gaacggcucc     1980

aucucccuca ccugccucgc ccucggcggc gucaugaucu uccucuccac cgccgucucc     2040

gcc                                                                   2043


<210>  1538
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short CDS opt

<400>  1538
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucggcua cgagaccgac     1020

gagaaccgcg ccaaggucga ggucaccccc aacucccccc gcgccgaggc cacccucggc     1080

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1140

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1200

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1320

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1380

ggcaagcucu ucuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1440

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagguccc cgccgagacc     1500

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagauc     1560

cccguccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1620

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1680

uucggcgacu ccuacaucgu caucggcguc ggcgacaaga agaucaccca ccacuggcac     1740

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1800

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgucuu caacucccuc     1860

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1920

ugguucuccc agauccucau cggcacccuc cucgucuggc ucggccucaa caccaagaac     1980

ggcuccaucu cccucaccug ccucgcccuc ggcggcguca ugaucuuccu cuccaccgcc     2040

gucuccgcc                                                             2049


<210>  1539
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short CDS opt

<400>  1539
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggccgcgg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1540
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short CDS opt

<400>  1540
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggccuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1541
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short CDS opt

<400>  1541
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggu accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1542
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short CDS opt

<400>  1542
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacacccaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1543
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short CDS opt

<400>  1543
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagauccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1544
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short CDS opt

<400>  1544
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccucgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1545
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short CDS opt

<400>  1545
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccacc cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1546
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short CDS opt

<400>  1546
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ucgucuccgc c                                               2061


<210>  1547
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short CDS opt

<400>  1547
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccgccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcc cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1548
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short CDS opt

<400>  1548
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccaa gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1549
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short CDS opt

<400>  1549
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccacccgcca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1550
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short CDS opt

<400>  1550
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcaccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1551
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short CDS opt

<400>  1551
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacuccc uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1552
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short CDS opt

<400>  1552
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcauc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1553
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short CDS opt

<400>  1553
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga uccuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggca ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1554
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short CDS opt

<400>  1554
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucucc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccac caucuccgac aucgccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgccgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1555
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short CDS opt

<400>  1555
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgagcucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1556
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short CDS opt

<400>  1556
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaucgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1557
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short CDS opt

<400>  1557
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcguca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cuccuucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1558
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short CDS opt

<400>  1558
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggcaagcu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1559
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short CDS opt

<400>  1559
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaaggacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1560
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short CDS opt

<400>  1560
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggag gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1561
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short CDS opt

<400>  1561
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccgcccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagacccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1562
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short CDS opt

<400>  1562
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg cgccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1563
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short CDS opt

<400>  1563
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgcccuc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1564
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short CDS opt

<400>  1564
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggucuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1565
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short CDS opt

<400>  1565
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcaucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cauccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1566
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short CDS opt

<400>  1566
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaccugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccau ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacgccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1567
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short CDS opt

<400>  1567
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc agcgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1568
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short CDS opt

<400>  1568
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

caggcccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1569
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short CDS opt

<400>  1569
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu cuccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gaggaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1570
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short CDS opt

<400>  1570
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacacc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag ggcaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1571
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short CDS opt

<400>  1571
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1572
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short CDS opt

<400>  1572
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgucga ggucacccgc       60

cgcggcaacg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccacca ugggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1573
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short CDS opt

<400>  1573
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucguc ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1574
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short CDS opt

<400>  1574
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucaac aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucguc ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1575
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short CDS opt

<400>  1575
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgucga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccgccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1576
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short CDS opt

<400>  1576
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgucga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgucau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1577
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short CDS opt

<400>  1577
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgucga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcgucaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggu accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1578
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short CDS opt

<400>  1578
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgucga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

cccaccaccc ucggcgucaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaucggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1579
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short CDS opt

<400>  1579
accuccaucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc acccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1580
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short CDS opt

<400>  1580
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaacc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaucggc     1020

cacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cguccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1581
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short CDS opt

<400>  1581
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaucggc     1020

cacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1582
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short CDS opt

<400>  1582
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaacc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacgagaac     1020

cgcgccaagg ucgaggucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1080

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1140

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1200

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1260

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1320

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1380

cucuucuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1440

uccuacuccc ucugcaccgc cgccuucacc uucaccaagg uccccgccga gacccuccac     1500

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1560

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1620

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1680

gacuccuaca ucgucaucgg cgucggcgac aagaagauca cccaccacug gcaccgcucc     1740

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1800

cucggcgaca ccgccuggga cuucggcucc gucggcggcg ucuucaacuc ccucggcaag     1860

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1920

ucccagaucc ucaucggcac ccuccucguc uggcucggcc ucaacaccaa gaacggcucc     1980

aucucccuca ccugccucgc ccucggcggc gucaugaucu uccucuccac cgccgucucc     2040

gcc                                                                   2043


<210>  1583
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short CDS opt

<400>  1583
accuccaucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1584
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short CDS opt

<400>  1584
accuccaucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca gcacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaucggc     1020

cacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ucugcaccgc cgccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1585
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short CDS opt

<400>  1585
accuccaucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu caucaagguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgucg ccgucgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucaccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacgagaac     1020

cgcgccaagg ucgaggucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1080

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1140

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1200

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1260

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1320

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1380

cucuucuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1440

uccuacuccc ucugcaccgc cgccuucacc uucaccaagg uccccgccga gacccuccac     1500

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1560

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1620

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1680

gacuccuaca ucgucaucgg cgucggcgac aagaagauca cccaccacug gcaccgcucc     1740

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1800

cucggcgaca ccgccuggga cuucggcucc gucggcggcg ucuucaacuc ccucggcaag     1860

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1920

ucccagaucc ucaucggcac ccuccucguc uggcucggcc ucaacaccaa gaacggcucc     1980

aucucccuca ccugccucgc ccucggcggc gucaugaucu uccucuccac cgccgucucc     2040

gcc                                                                   2043


<210>  1586
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short CDS opt

<400>  1586
accuccaucg gcaucaucgg ccuccuccuc accaccgcca uggccgccga gaucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcuccgacg ccggcaaggc caucuccuuc      120

gccaccaccc ucggcgucaa caagugccac guccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgagaccc gccgcucccg ccgcuccguc ucccuccgcu accacuacac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca agaagcaccu caucaugguc      420

gagaacugga ucuuccgcaa ccccggcuuc gccaucgucu ccgucgccau caccuggcuc      480

augggcuccc ucaccuccca gaaggucauc uaccucguca ugaucguccu caucgucccc      540

gccuacucca ucuccugcau cggcgucucc aaccgcgacc ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgagau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc augaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc ccucuccgac auggccuccg ccucccgcug ccccacccag      780

ggcgagcccu cccucgacaa gcaguccgac acccaguccg ucugcaagcg cacccucggc      840

gaccgcggcu ggggcaacgg cugcggcauc uucggcaagg gcucccucgu caccugcucc      900

aaguucaccu gcugcaagaa gaugcccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc uccccgucca cggcucccag cacuccggca ugaucgucaa cgacaucggc     1020

cacgagaccg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguccgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1587
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short CDS opt

<400>  1587
uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc       60

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      120

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      180

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      240

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      300

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      360

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      420

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      480

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      540

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      600

accugggucg acgucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      660

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      720

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      780

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      840

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      900

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc      960

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1020

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1080

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1140

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1200

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1260

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1320

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1380

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1440

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1500

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1560

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1620

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1680

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1740

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1800

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1860

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1920

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     1980

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2040

uccaccgccg ucuccgcc                                                   2058


<210>  1588
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short CDS opt

<400>  1588
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gcccgccgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1589
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short CDS opt

<400>  1589
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga uguuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1590
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short CDS opt

<400>  1590
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaugac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  1591
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short CDS opt

<400>  1591
accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc       60

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      120

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      240

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      300

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      360

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      480

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      540

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      600

ggcaccuggg ucgacgucgu ccucgagcac ggcggcugcg ucaccgccau ggcccaggac      660

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      720

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      780

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      900

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac      960

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugcucgucaa cgacaccggc     1020

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgccca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgcca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga ccgucgacgg caccgucacc gucgagggcc aguacggcgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1860

cucaacuccc ucggcaaggg cauccaccag aucaucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     1980

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2040

cucuccaccg ccgucuccgg c                                               2061


<210>  1592
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short CDS opt

<400>  1592
ggcgccgacg ccuccaucgg caucgucucc cuccuccuca ccaccgucau ggccgccgag       60

aucacccgcc gcggcuccgc cuacuacaug uaccucgacc gcuccgacgc cggcaaggcc      120

aucuccuucg ucaccacccu cggcgucaac aagugccacg uccagaucau ggaccucggc      180

cacaugugcg acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc      240

gacgacgucg acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac      300

cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc      360

cgcaagcucc agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc      420

aucaaggucg agaacuggau cuuccgcaac cccggcuucg cccucgucgc cgucgccauc      480

gccuggcucc ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc      540

aucgcccccg ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc      600

auguccggcg gcaccugggu cgacgucguc cucgagcacg gcggcugcgu caccgucaug      660

gcccaggaca agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc      720

gagguccgcu ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc      780

cccacccagg gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc      840

acccucgucg accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc      900

accugcgcca aguucaccug cuccaagaag augaccggca aguccaucca gcccgagaac      960

cucgaguacc gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac     1020

gacgagaacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1080

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1140

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1200

aucccccucc ccuggcacuc cggcgccgac accgagaccc cccacuggaa caacaaggag     1260

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1320

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1380

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1440

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1500

acccuccacg gcaccgucac cgucgagguc caguacgccg gccgcgacgg ccccugcaag     1560

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1620

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1680

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1740

caccgcuccg gcuccaucau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1800

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1860

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1920

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     1980

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     2040

gccgucuccg cc                                                         2052


<210>  1593
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short CDS opt

<400>  1593
ggcgccgaca ccuccaucgg caucaucggc cuccuccuca ccaccgccau ggccgccgag       60

aucacccgcc gcggcuccgc cuacuacaug uaccucgacc gcuccgacgc cggcaaggcc      120

aucuccuucg ccaccaccau gggcgucaac aagugccacg uccagaucau ggaccucggc      180

cacaugugcg acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc      240

gacgacgucg acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac      300

cacaagaagg gcgaggcccg ccccucccgc cgcgccguca cccuccccuc ccacuccacc      360

cgcaagcucc agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc      420

aucaaggucg agaacuggau ccuccgcaac cccggcuuca ccgucgccgc ccucgccaug      480

gccuggcucc ucggcaacuc caccucccag cgcgucaucu accucgucau gauccuccuc      540

aucgcccccg ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc      600

auguccggcg gcaccugggu cgacgucguc cucgagcacg gcggcugcgu caccgucaug      660

gcccaggaca agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc      720

gagguccgcu ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc      780

cccacccagg gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc      840

acccucgucg accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc      900

accugcgcca aguucaccug cuccaagaag augaccggca aguccaucca gcccgagaac      960

cucgaguacc gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac     1020

gacaucggcc acgagaccga cgagaaccgc gccaaggucg aggucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uucuccggcc accucaagug ccgcaucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

uccaaggucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

cgcgacggcc ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccauc aucaccgagu ccaccgagaa cucccgcaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgacaag     1740

aagaucaccc accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc     1800

guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc     1860

ggcggcgucu ucaacucccu cggcaagggc guccaccagg ucuucggcgc cgccuucaag     1920

ucccucuucg gcggcauguc cugguucucc cagauccuca ucggcacccu ccucgucugg     1980

cucggccuca acaccaagaa cggcuccauc ucccucgccu gccucgcccu cggcggcgcc     2040

augaucuucc ucuccaccgc cgucuccgcc                                      2070


<210>  1594
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE CDS opt

<400>  1594
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1595
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE CDS opt

<400>  1595
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1596
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE CDS opt

<400>  1596
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag      540

gucgagguca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc      600

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug      660

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac      720

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag      780

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac      840

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc      900

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc      960

cucugcaccg ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc     1020

accgucgagg uccaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcc     1080

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1140

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1200

aucgucaucg gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc     1260

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1320

accgccuggg acuucggcuc c                                               1341


<210>  1597
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE CDS opt

<400>  1597
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1598
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE CDS opt

<400>  1598
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggcuacga gaccgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

aagcucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caagaucccc     1080

guccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1599
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE CDS opt

<400>  1599
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugacc gucaacgaca ucggcuacga gaccgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

aagcucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caagaucccc     1080

guccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1600
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE CDS opt

<400>  1600
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgacg agaaccgcgc caaggucgag      540

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc      600

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac      660

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc      720

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc      780

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc      840

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac      900

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc      960

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1020

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1080

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1140

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1200

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1260

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1320

ugggacuucg gcucc                                                      1335


<210>  1601
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE CDS opt

<400>  1601
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc ggcuacgaga ccgacgagaa ccgcgccaag      540

gucgagguca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc      600

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug      660

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac      720

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag      780

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac      840

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggcaa gcucuucucc      900

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc      960

cucugcaccg ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc     1020

accgucgagg uccaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcc     1080

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1140

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1200

aucgucaucg gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc     1260

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1320

accgccuggg acuucggcuc c                                               1341


<210>  1602
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE CDS opt

<400>  1602
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cucccuccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1603
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE CDS opt

<400>  1603
uccaagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1604
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE CDS opt

<400>  1604
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cugguaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1605
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE CDS opt

<400>  1605
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

aagcucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1606
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE CDS opt

<400>  1606
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacac ccagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1607
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE CDS opt

<400>  1607
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgaga uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1608
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE CDS opt

<400>  1608
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgcccg ccgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1609
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE CDS opt

<400>  1609
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggu cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1610
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE CDS opt

<400>  1610
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccau cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1611
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE CDS opt

<400>  1611
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcaucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1612
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE CDS opt

<400>  1612
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccucgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1613
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE CDS opt

<400>  1613
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cacccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1614
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE CDS opt

<400>  1614
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caagguccuc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1615
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE CDS opt

<400>  1615
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggaggccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1616
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE CDS opt

<400>  1616
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccgc ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1617
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE CDS opt

<400>  1617
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucuccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccaccaucu ccgacaucgc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgccg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1618
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE CDS opt

<400>  1618
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucucca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgagga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1619
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE CDS opt

<400>  1619
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gcuccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1620
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE CDS opt

<400>  1620
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggca ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcaucc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1621
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE CDS opt

<400>  1621
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgucaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1622
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE CDS opt

<400>  1622
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcgccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1623
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE CDS opt

<400>  1623
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acaccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgagggcac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1624
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE CDS opt

<400>  1624
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gccauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugcuc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc gcccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgccaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagaccguc     1020

gacggcaccg ucaccgucga gggccaguac ggcggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1625
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE CDS opt

<400>  1625
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc cgccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1626
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE CDS opt

<400>  1626
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucaacaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1627
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE CDS opt

<400>  1627
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcgc cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1628
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE CDS opt

<400>  1628
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1629
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE CDS opt

<400>  1629
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cugguaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1630
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE CDS opt

<400>  1630
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1631
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE CDS opt

<400>  1631
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1632
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE CDS opt

<400>  1632
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgucugc accgccgcca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1633
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE CDS opt

<400>  1633
ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgacg agaaccgcgc caaggucgag      540

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc      600

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac      660

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacuccggc      720

gccgacaccg agacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc      780

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc      840

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac      900

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc      960

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1020

gagguccagu acgccggccg cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1080

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1140

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1200

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caucaucggc     1260

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1320

ugggacuucg gcucc                                                      1335


<210>  1634
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE CDS opt

<400>  1634
ucccagaagg ucaucuaccu cgucaugauc guccucaucg uccccgccua cuccaucucc       60

ugcaucggcg ucuccaaccg cgaccucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gagauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaugac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccucccucu ccgacauggc cuccgccucc cgcugcccca cccagggcga gcccucccuc      300

gacaagcagu ccgacaccca guccgucugc aagcgcaccc ucggcgaccg cggcuggggc      360

aacggcugcg gcaucuucgg caagggcucc cucgucaccu gcuccaaguu caccugcugc      420

aagaagaugc ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucccc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccagucc gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1635
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE CDS opt

<400>  1635
cgccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      540

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1636
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE CDS opt

<400>  1636
ucccagcgcg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc       60

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      120

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      180

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      240

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      300

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      360

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      420

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      480

guccacggcu cccagcacuc cggcaugauc gucaacgaca ucggccacga gaccgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuucu ccggccaccu caagugccgc aucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucucca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggccgcg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccaucauca ccgaguccac cgagaacucc cgcaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc ucc                                  1353


<210>  1637
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  1637
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

auaguguuag agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac      720

auugagcugg ucaccacgac uguuucaaau auggcugaag ugcguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aaguggaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guggaguuua aagacgcgca cgcaaagagg cagaccguag uugugcuggg cagccaggag     1380

ggggccgucc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagacccug acuccugucg gcagguuaau uacagccaac     1680

ccagugauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggagugcuga uuuuccuguc aacggccguu     2100

agugcugacg ucggcugcuc uguggauuuu agcaaa                               2136


<210>  1638
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS opt

<400>  1638
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca cuugugacgc caccauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

acaaccagua cuuggguggu cuaugguaca ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacccu accgucccau ucuacgagga agcuccaaac ucggucgcag      420

accuggcugg agucacgcga auacacaaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguacu      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac cuggguugac      660

guggucuuag agcauggcgg gugugugaca gugauggcac aggauaagcc aaccgucgac      720

auagagcugg uuacgacuac agugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cccaaggaga agccuaccuu      840

gacaaacaga gcgauacuca guaugugugc aagagaacau uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugaccu gcgcaaaguu ugcuugcucu      960

aagaaaauga cggggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua ccggccauga gacagacgag     1080

aaucgagcca aagucgaaau cacuccaaac ucaccccgcg cugaggccac ccuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaacagggc ucgacuuuag ugaucuguau     1200

uacuuaacua ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauaccgga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auacugcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ggccgcauuc acauuuacca aaauaccugc ggaaacucuc     1560

cauggcaccg ugacaguuga ggugcaguac gcugguaccg acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacucug acaccugugg gcagguuaau cacggccaac     1680

ccaguuauua ccgaaaguac ugaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacacacca cuggcaucgu     1800

ucuggaucaa ccaucgguaa ggcuuuugag gccacagugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacugccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacccuccug auguggcuug gccugaaugc aaagaacggu     2040

agcauauccu ugaugugucu cgccuuaggg ggaguucuga ucuuccuguc aacggcuguc     2100

agugccgacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1639
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS opt

<400>  1639
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacauugggg      180

gugaauaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgucacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauaa aaguggaaaa uuggaucuuu      480

agaaacccgg gauucgcucu gguugccgug gcgauugcau gguugcucgg caguuccaca      540

ucucagaagg ucaucuaccu ggugaugauu cugcucaucg cccccgcuua uagcauaagg      600

ugcauuggcg uauccaaucg ggauuuuguu gaggggaugu caggagguac uuggguggac      660

gucguguuag aacacggcgg gugugugacc gucauggccc aggauaaacc uacaguugac      720

aucgagcugg ugaccacgac uguaaguaac auggcagagg ugcguagcua cugcuacgag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgucugc aaaagaacuu ugguggacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuaguuacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauccagccc gaaaaccucg aguaucggau caugcugucg     1020

gugcacggau cucaacauuc cgguaugauu ggcuacgaaa ccgaugagga ccgagcaaag     1080

gucgagguga caccuaauuc accacgcgcu gaagccacuc uugggggauu cggcagucug     1140

gguuuggauu gugagcccag aaccgggcuc gacuuuagcg aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu acauaaggaa ugguuccacg acauaccucu cccauggcac     1260

gcuggcgccg auacuggaac cccgcauugg aacaauaaag aggcacuugu ugaauuuaag     1320

gacgcccacg cuaagaggca gacagugguc gugcugggcu cccaggaggg ggcgguccau     1380

accgcauugg ccggagcucu agaggccgaa auggauggug caaaaggccg gcuguucucu     1440

gggcaccuca agugccgccu gaaaauggac aaacuuagac ugaagggagu gagcuauuca     1500

uuguguacug ccgcuuuuac guucacaaag guucccgccg agacccucca uggcacugug     1560

accguagaag uccaguacgc agguacagac gggccuugca agaucccagu gcaaauggcg     1620

guugauaugc agacccugac ucccgugggc agguuaauua cagcuaaccc ugucaucacg     1680

gagaguaccg aaaauuccaa aaugaugcug gagcuagacc cacccuuugg agauagcuac     1740

auagugauug gcguagggga caagaaaauc acucaccacu ggcaucguuc uggaucaaca     1800

aucgguaagg ccuucgaggc aaccgugcgg ggcgccaaac gaauggcugu ucucggggau     1860

acagccuggg acuucggaag ugucggcggu guguuuaaca gccuggggaa gggcauucac     1920

cagaucuucg gagcagcuuu uaagucccuu uucggcggga ugucuugguu uucgcaaauu     1980

uugauaggaa cucugcucgu guggcugggc cuuaacacca aaaaugguag caucucccug     2040

acguguuugg cccucggggg agucaugauu uuccugucaa ccgcgguuag ugccgaugug     2100

ggcugcucug uggacuuuag caag                                            2124


<210>  1640
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) CDS opt

<400>  1640
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1641
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  1641
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc a                                               2061


<210>  1642
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) CDS opt

<400>  1642
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacacuugu      180

gaugccacca ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacacaac caguacuugg guggucuaug gcacauguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acccuaccgu cccauucuac gaggaaacuc      360

caaacucggu cgcagaccug gcuggaguca cgcgaguaca caaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacuuccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaaccuggg uugauguggu cuuagaacau gguggcugug ugacagugau ggcucaggac      660

aagccaaccg ucgauauuga gcugguuacg acuacagugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacccaa      780

ggggaggccu accuugacaa gcagagcgac acucaguaug ugugcaaaag gacauugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gaccugcgcg      900

aaauuugccu gcucuaagaa aaugacgggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacaccggu     1020

caugaaacag augagaaccg cgcaaagguc gagauuacuc caaauucccc cagagcugaa     1080

gccacccuug gcggguucgg aucacugggc uuggacugug agccuaggac aggucucgau     1140

uuuagugacc uguauuacuu aacuaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca ccggcacacc ccauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac cguugugguc     1320

cugggaagcc aggagggcgc ggugcauacu gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacggccg cuuucacauu uaccaagauc     1500

ccugccgaga cucuccaugg aaccgugaca guugaagugc aguacgcagg caccgacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cucugacacc uguggggagg     1620

uuaauuacgg ccaacccagu uauaaccgag aguacugaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucaca     1740

caccacuggc aucggucugg gucaaccaua ggaaaggcau uugaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacu gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggaccc uccugaugug gcuuggacug     1980

aacgccaaga auggcagcau cuccuugaug ugucucgcuc uggguggggu uuuaauuuuc     2040

cugucaacgg cagucagugc c                                               2061


<210>  1643
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) CDS opt

<400>  1643
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uauuaaggug      420

gagaacugga ucuuuagaaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uuaggugcau cggcguaucc aaccgggacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcac gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcgc      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag aacuuuggug      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcaucc agcccgaaaa ucucgaauau      960

cggauuaugc ugucggugca cgggucucaa cauuccggaa ugaucgguua cgagaccgac     1020

gaagaucgug cgaaggucga ggugacaccu aacucaccac gcgccgaggc aacucuuggc     1080

ggguucggaa gucugggcuu ggacugugaa cccagaaccg gucucgauuu uagcgaccug     1140

uauuacuuaa caaugaauaa caaacacugg cugguacaua aggagugguu ccacgauauc     1200

ccucucccau ggcacgcugg ggccgacacu ggcaccccgc auuggaauaa caaagaagcu     1260

cuuguugagu uuaaggaugc ccacgcaaaa aggcagacag uggucgugcu gggaucccag     1320

gaaggcgccg uccauaccgc uuuggcgggg gcacuagagg ccgagaugga cggagcuaag     1380

ggucggcugu ucucuggcca ccucaagugc cgacugaaaa uggauaaacu ucgccugaag     1440

ggggugagcu auucauugug uacugccgca uuuacguuca caaagguucc cgccgaaacc     1500

cuccauggaa cugugaccgu agagguccag uacgcuggca cagacggucc uugcaaaauu     1560

ccagugcaaa uggccguuga caugcagacc cugacucccg uggggagauu aauaacagca     1620

aauccuguca ucacggaaag uaccgagaac uccaagauga ugcuggaacu agauccaccc     1680

uuuggcgaca gcuacauugu gaucggagua ggcgauaaga aaauaacuca ccacuggcau     1740

aggucugggu caacaauugg aaaggcguuc gaggcuaccg ugcggggugc caaacgcaug     1800

gcaguucucg gcgacacagc cugggauuuc gggagugucg gaggcguguu uaauagccug     1860

gguaagggga uccaccagau cuucggcgcu gccuuuaaau cccuuuucgg aggcaugucu     1920

ugguuuucgc aaauuuugau cgggacucug cucguguggc ugggacuuaa caccaagaac     1980

ggcagcauuu cccugacgug uuuggcacuc ggugggguca ugauauuccu gucaaccgcu     2040

guuagugcc                                                             2049


<210>  1644
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) CDS opt

<400>  1644
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1645
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS opt

<400>  1645
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

aucgugcugg aacacggcgg gugugucaca gugauggcac aagacaaacc uacuguugau      180

auugaguugg ugacgaccac agucaguaau auggccgaag ugcggagcua cugcuaugag      240

gcguccaucu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguaugc aaacgaacac ugguugaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauacaaccg gagaaucugg aguacagaau caugcuuagc      480

guccauggau cccagcacuc agguaugauu gugaacgaua caggccauga aaccgacgag      540

aauagggcua agguggaaau cacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guccauaagg aaugguuuca cgacauuccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guugaguuca aggaugcaca cgccaagaga cagacagugg uaguguuggg cucccaggaa      840

ggggcugucc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugaguuacu cucucugcac ggccgcauuu acuuucacaa agauccccgc ugagacccug     1020

cacggcacug uuacagugga aguccaauau gccgguaccg acgggccuug uaaagugcca     1080

gcucagaugg ccguagauau gcagacguua accccgguug gccgacugau aacagcaaau     1140

cccgugauua cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acaucgucau uggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguca ggggcgcgaa acggauggca     1320

gugcuggggg acacagccug ggauuuugga uca                                  1353


<210>  1646
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS opt

<400>  1646
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guucuggggg acacagccug ggauuuugga uca                                  1353


<210>  1647
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS opt

<400>  1647
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc ggcuaugaaa ccgaugagga cagggccaag      540

guggaaguca cgcccaacag uccucgggcu gaggcaacuc ugggaggguu uggcagcuug      600

ggucucgauu gcgagccacg caccgggcug gacuucucug aucuguacua ccucacaaug      660

aauaacaaac auugguuagu ucacaaggaa ugguuucaug acauuccccu gccuuggcac      720

gccggagcug acacuggcac cccacacugg aauaacaaag aggcccuugu ggaguucaag      780

gaugcgcaug caaagagaca gacaguagug gucuugggcu cccaggaagg ggccgugcac      840

accgcucugg ccggagcacu agaagcugag auggacggug ccaaaggcag gcucuuuuca      900

gggcaucuga agugucggcu uaaaauggau aagcugcgcu ugaaaggagu uagcuauagu      960

cucugcacgg cggccuucac uuuuacaaag gugcccgcag agacccugca cggcacuguc     1020

acaguggaag uacaauacgc ugguaccgac gggccuugua aaaucccagu ucagauggcc     1080

guggauaugc agacguuaac cccggucggc cgacugauua cagcuaaucc cgugaucacu     1140

gagucuaccg aaaacuccaa gaugaugcuc gagcuugacc cuccauucgg agauagcuau     1200

auagucauug gcguggggga caagaaaauc acacaccauu ggcacagauc gggaucuacu     1260

auugguaagg ccuuugaagc aaccguuagg ggcgccaaac ggauggcugu gcugggggau     1320

acagcguggg acuucggauc c                                               1341


<210>  1648
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) CDS opt

<400>  1648
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guucuggggg acacagccug ggauuuugga uca                                  1353


<210>  1649
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) CDS opt

<400>  1649
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug aguggaaagg gcucucuagu cacaugcgcg      900

aaauucgccu gcuccaagaa aaugaccggc aagagcauuc agccugaaaa ccucgaguac      960

cguaucaugc ugucggugca cgggucucaa cacuccggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucacc cagagcugaa     1080

gccacucuug gcggguuugg aagucugggc uuggacugug agccuaggac cggucucgau     1140

uucagcgacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuucacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga guucaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggauccc aggaaggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaag     1440

cugagacuga aagggguguc auauaguuug uguacugccg cuuuuacguu cacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag uccaccgaaa acagcaagau gaugcuggag     1680

cuagaucccc cguuuggcga cucuuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucagg gaguacaauu ggaaaggcau ucgaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu uugggagcgu aggaggcgcu     1860

cuuaauuccc uugguaaggg gauccaccag aucuucggcg ccgcguucaa aucuuuguuu     1920

ggaggcaugu cgugguucag ccaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcuccau uucauugaug ugucucgccc uggguggggu guuaauauuu     2040

cugaguacgg cuguuucugc a                                               2061


<210>  1650
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) CDS opt

<400>  1650
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacacuugu      180

gaugccacca ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacacaac caguacuugg guggucuaug gcacauguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acccuaccgu cccauucuac gaggaaacuc      360

caaacucggu cgcagaccug gcuggaguca cgcgaguaca caaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacuuccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaaccuggg uugauguggu cuuagaacau gguggcugug ugacagugau ggcucaggac      660

aagccaaccg ucgauauuga gcugguuacg acuacagugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacccaa      780

ggggaggccu accuugacaa gcagagcgac acucaguaug ugugcaaaag gacauugguc      840

gaucggggau ggggcaaugg uugugggcug aguggaaagg gcucucuagu gaccugcgcg      900

aaauucgccu gcuccaagaa aaugacgggc aagagcauac agccugaaaa ccucgaguac      960

cguauuaugc ugucgguuca cgggucucaa cacuccggaa ugaucgugaa ugacaccggu     1020

caugaaacag augagaaccg cgcaaagguc gagauuacuc caaauucacc cagagcugaa     1080

gccacccuug gcggguuugg aagucugggc uuggacugug agccuaggac aggucucgau     1140

uucagcgacc uguauuacuu aacuaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuucacgaua ucccacuccc guggcacgcu ggggccgaca ccggcacacc ccauuggaac     1260

aauaaagagg cacuuguaga guucaaggau gcccacgcua aacggcagac cguugugguc     1320

cugggauccc aggaaggcgc ggugcauacu gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaag     1440

cugagacuga aaggggucuc auauaguuug uguacggccg cuuuuacauu caccaagauc     1500

ccugccgaga cucuccaugg aaccgugaca guugaagugc aguacgcagg caccgacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cucugacacc uguggggagg     1620

uuaauuacgg ccaacccagu uauaaccgag uccacugaaa acagcaagau gaugcuggag     1680

cuagaucccc cguuuggcga cucuuacauc gugauuggag ucggcgagaa aaagaucaca     1740

caccacuggc aucggucagg gaguaccaua ggaaaggcau ucgaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacu gcaugggacu uugggagcgu gggaggcgcu     1860

cuuaauuccc uugguaaggg gauucaccag aucuucggcg ccgcguucaa aucuuuguuu     1920

ggaggcaugu cgugguucag ccaaauccug auugggaccc uccugaugug gcuuggacug     1980

aacgccaaga auggcuccau cucauugaug ugucucgcuc uggguggggu uuuaauuuuu     2040

cugaguacgg cagucucugc c                                               2061


<210>  1651
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) CDS opt

<400>  1651
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uauuaaggug      420

gagaacugga ucuuuagaaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uuaggugcau cggcguaucc aaccgggacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcac gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcgc      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag aacuuuggug      840

gauaggggau ggggcaacgg uugugggcug ucuggaaagg gcucccuagu uacaugcgcc      900

aaauuuaccu gcagcaagaa gaugacgggc aaaucgaucc agcccgaaaa ucucgaauau      960

cggauuaugc ugucugugca cgggucccaa cauucaggaa ugaucgguua cgagaccgac     1020

gaagaucgug cgaaggucga ggugacaccu aacaguccac gcgccgaggc aacucuuggc     1080

ggguucggaa gccugggcuu ggacugugaa cccagaaccg gucucgauuu cuccgaccug     1140

uauuacuuaa caaugaauaa caaacacugg cugguacaua aggagugguu ucacgauauc     1200

ccucucccau ggcacgcugg ggccgacacu ggcaccccgc auuggaauaa caaagaagcu     1260

cuuguugagu ucaaggaugc ccacgcaaaa aggcagacag uggucgugcu gggaucucag     1320

gaaggcgccg uccauaccgc uuuggcgggg gcacuagagg ccgagaugga cggagcuaag     1380

ggucggcugu uuagcggcca ccucaagugc cgacugaaaa uggauaaacu ucgccugaag     1440

ggggugucau auaguuugug uacugccgca uucacguuua caaagguucc cgccgaaacc     1500

cuccauggaa cugugaccgu agagguccag uacgcuggca cagacggucc uugcaaaauu     1560

ccagugcaaa uggccguuga caugcagacc cugacucccg uggggagauu aauaacagca     1620

aauccuguca ucacggaauc caccgagaac agcaagauga ugcuggaacu agauccaccc     1680

uucggcgacu cuuacauugu gaucggagua ggcgauaaga aaauaacuca ccacuggcau     1740

aggucaggga guacaauugg aaaggcguuu gaggcuaccg ugcggggugc caaacgcaug     1800

gcaguucucg gcgacacagc cugggauuuc gggagcgucg gaggcguguu caauucccug     1860

gguaagggga uccaccagau cuuuggcgcu gccuucaaau cucuuuuugg aggcaugucg     1920

ugguucagcc aaauuuugau cgggacucug cucguguggc ugggacuuaa caccaagaac     1980

ggcuccauuu cacugacgug uuuggcacuc ggugggguca ugauauuucu gaguaccgcu     2040

guuucugcc                                                             2049


<210>  1652
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) CDS opt

<400>  1652
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug aguggaaagg gcucucuagu gacaugcgcg      900

aaauucgccu gcuccaagaa aaugaccggc aagagcauac agccugaaaa ccucgaguac      960

cguauuaugc ugucgguuca cgggucucaa cacuccggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucacc cagagcugaa     1080

gccacucuug gcggguuugg aagucugggc uuggacugug agccuaggac cggucucgau     1140

uucagcgacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuucacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga guucaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggauccc aggaaggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaag     1440

cugagacuga aaggggucuc auauaguuug uguacugccg cuuuuacguu cacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag uccaccgaaa acagcaagau gaugcuggag     1680

cuagaucccc cguuuggcga cucuuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucagg gaguacaaua ggaaaggcau ucgaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu uugggagcgu gggaggcgcu     1860

cuuaauuccc uugguaaggg gauucaccag aucuucggcg ccgcguucaa aucuuuguuu     1920

ggaggcaugu cgugguucag ccaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcuccau cucauugaug ugucucgccc uggguggggu uuuaauuuuu     2040

cugaguacgg cugucucugc a                                               2061


<210>  1653
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) CDS opt

<400>  1653
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuca ggacacgggu     1020

caugaaaccg augagaaucg cgcaaaggug gagaucacac caaacucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaau aacaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaau     1260

aacaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aauacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca gauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaauccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaacagcc uugguaaggg gauccaccaa aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcagauucug aucgggacuc uccugaugug gcuuggacug     1980

aauaccaaga acggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc a                                               2061


<210>  1654
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) CDS opt

<400>  1654
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacacuugu      180

gaugccacca ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacacaac caguacuugg guggucuaug gcacauguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acccuaccgu cccauucuac gaggaaacuc      360

caaacucggu cgcagaccug gcuggaguca cgcgaguaca caaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacuuccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaaccuggg uugauguggu cuuagaacau gguggcugug ugacagugau ggcucaggac      660

aagccaaccg ucgauauuga gcugguuacg acuacagugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacccaa      780

ggggaggccu accuugacaa gcagagcgac acucaguaug ugugcaaaag gacauugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gaccugcgcg      900

aaauuugccu gcucuaagaa aaugacgggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugca ggacaccggu     1020

caugaaacag augagaaucg cgcaaagguc gagauuacuc caaacucccc cagagcugaa     1080

gccacccuug gcggguucgg aucacugggc uuggacugug agccuaggac aggucucgau     1140

uuuagugacc uguauuacuu aacuaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca ccggcacacc ccauuggaau     1260

aacaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac cguugugguc     1320

cugggaagcc aggagggcgc ggugcauacu gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacggccg cuuucacauu uaccaagauc     1500

ccugccgaga cucuccaugg aaccgugaca guugaagugc aauacgcagg caccgacggu     1560

ccaugcaaag uacccgcgca gauggcuguc gacaugcaga cucugacacc uguggggagg     1620

uuaauuacgg ccaauccagu uauaaccgag aguacugaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucaca     1740

caccacuggc aucggucugg gucaaccaua ggaaaggcau uugaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacu gcaugggacu ucgggagugu gggaggcgcu     1860

cugaacagcc uugguaaggg gauucaccaa aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcagauccug auugggaccc uccugaugug gcuuggacug     1980

aaugccaaga acggcagcau cuccuugaug ugucucgcuc uggguggggu uuuaauuuuc     2040

cugucaacgg cagucagugc c                                               2061


<210>  1655
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) CDS opt

<400>  1655
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugca ggacacgggu     1020

caugaaaccg augagaaucg cgcaaagguc gagauuacac caaacucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaau     1260

aacaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aauacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca gauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaauccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaacagcc uugguaaggg gauucaccaa aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcagauccug auugggacuc uccugaugug gcuuggacug     1980

aauaccaaga acggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1656
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1656
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacacuc ggaaaggcau uuaguaccac acugaaaggu     1800

gcccagcgcc uugcugccuu gggcgauacu gcaugggacu ucgggagcau uggaggcguc     1860

uucaauucca ucgguagagc ugugcaccaa guauuugggg gcgccuucag gacccuguuu     1920

ggaggcaugu cuuggaucac gcaggggcuc augggagcgc ugcuucugug gaugggcgug     1980

aacgcccgug aucggucgau ugcuuuggca uuccucgcca ccgguggggu ucuggucuuu     2040

uuagcuacaa augugcaugc c                                               2061


<210>  1657
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1657
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacacuugu      180

gaugccacca ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacacaac caguacuugg guggucuaug gcacauguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acccuaccgu cccauucuac gaggaaacuc      360

caaacucggu cgcagaccug gcuggaguca cgcgaguaca caaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacuuccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaaccuggg uugauguggu cuuagaacau gguggcugug ugacagugau ggcucaggac      660

aagccaaccg ucgauauuga gcugguuacg acuacagugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacccaa      780

ggggaggccu accuugacaa gcagagcgac acucaguaug ugugcaaaag gacauugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gaccugcgcg      900

aaauuugccu gcucuaagaa aaugacgggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacaccggu     1020

caugaaacag augagaaccg cgcaaagguc gagauuacuc caaauucccc cagagcugaa     1080

gccacccuug gcggguucgg aucacugggc uuggacugug agccuaggac aggucucgau     1140

uuuagugacc uguauuacuu aacuaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca ccggcacacc ccauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac cguugugguc     1320

cugggaagcc aggagggcgc ggugcauacu gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacggccg cuuucacauu uaccaagauc     1500

ccugccgaga cucuccaugg aaccgugaca guugaagugc aguacgcagg caccgacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cucugacacc uguggggagg     1620

uuaauuacgg ccaacccagu uauaaccgag aguacugaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucaca     1740

caccacuggc aucggucugg gucaacccuc ggaaaggcau uuaguacaac ucugaaaggu     1800

gcccagcgcc uugcugccuu gggcgauacc gcaugggacu ucgggagcau aggaggcgug     1860

uucaauucca uugguagagc uguacaccaa guguuugggg gcgccuucag gacgcuguuu     1920

ggaggcaugu cuuggaucac ccaggggcuc augggagcgc ugcuucugug gaugggcguu     1980

aacgcccgug aucggucgau cgcuuuggca uuccucgcca cagguggggu ccugguguuu     2040

uuagcuacua augugcaugc c                                               2061


<210>  1658
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       CDS opt

<400>  1658
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uauuaaggug      420

gagaacugga ucuuuagaaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uuaggugcau cggcguaucc aaccgggacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcac gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcgc      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag aacuuuggug      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcaucc agcccgaaaa ucucgaauau      960

cggauuaugc ugucggugca cgggucucaa cauuccggaa ugaucgguua cgagaccgac     1020

gaagaucgug cgaaggucga ggugacaccu aacucaccac gcgccgaggc aacucuuggc     1080

ggguucggaa gucugggcuu ggacugugaa cccagaaccg gucucgauuu uagcgaccug     1140

uauuacuuaa caaugaauaa caaacacugg cugguacaua aggagugguu ccacgauauc     1200

ccucucccau ggcacgcugg ggccgacacu ggcaccccgc auuggaauaa caaagaagcu     1260

cuuguugagu uuaaggaugc ccacgcaaaa aggcagacag uggucgugcu gggaucccag     1320

gaaggcgccg uccauaccgc uuuggcgggg gcacuagagg ccgagaugga cggagcuaag     1380

ggucggcugu ucucuggcca ccucaagugc cgacugaaaa uggauaaacu ucgccugaag     1440

ggggugagcu auucauugug uacugccgca uuuacguuca caaagguucc cgccgaaacc     1500

cuccauggaa cugugaccgu agagguccag uacgcuggca cagacggucc uugcaaaauu     1560

ccagugcaaa uggccguuga caugcagacc cugacucccg uggggagauu aauaacagca     1620

aauccuguca ucacggaaag uaccgagaac uccaagauga ugcuggaacu agauccaccc     1680

uuuggcgaca gcuacauugu gaucggagua ggcgauaaga aaauaacuca ccacuggcau     1740

aggucugggu caacacucgg aaaggcguuc aguaccacac ugaaaggugc ucagcggcuu     1800

gccgcauugg gcgacacugc cugggauuuc gggagcauug gaggcguguu uaauuccauc     1860

ggucgcgcug uucaccaagu cuucgggggc gccuuuagaa cccuguucgg aggcaugucu     1920

uggaucacgc aggggcucau gggagcacug cuucugugga ugggcgugaa cgcuagggac     1980

cguucgauug ccuuggcguu ucucgccacc gguggggugc uggucuucuu agcuacaaac     2040

guucaugca                                                             2049


<210>  1659
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1659
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacacuc ggaaaggcau uuaguaccac acugaaaggu     1800

gcccagcgcc uugcugccuu gggcgauacu gcaugggacu ucgggagcau aggaggcgug     1860

uucaauucca uugguagagc uguacaccaa guguuugggg gcgccuucag gacccuguuu     1920

ggaggcaugu cuuggaucac gcaggggcuc augggagcgc ugcuucugug gaugggcguu     1980

aacgcccgug aucggucgau cgcuuuggca uuccucgcca ccgguggggu ccugguguuu     2040

uuagcuacaa augugcaugc c                                               2061


<210>  1660
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  1660
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

auaguguuag agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac      720

auugagcugg ucaccacgac uguuucaaau auggcugaag ugcguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aaguggaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guggaguuua aagacgcgca cgcaaagagg cagaccguag uugugcuggg cagccaggag     1380

ggggccgucc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagacccug acuccugucg gcagguuaau uacagccaac     1680

ccagugauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggagugcuga uuuuccuguc aacggccguu     2100

agugcugacg ucggcugcuc uguggauuuu agcaaa                               2136


<210>  1661
<211>  2133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1661
aucauuaacg ccagaaagga gaaaaagagg cggggcgcug acaccagcgu ggggauaguc       60

ggacugcucc uuacaacugc aauggccgcg gaaguuacgc gccgagguuc cgcuuacuau      120

auguaccugg aucguaauga cgccggcgag gcaaucucuu uccccaccac auuggggaug      180

aacaaaugcu auauucagau cauggauuua ggacacaugu gugacgccac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaauacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaga aaggggaggc uagaaggagc      360

cggcgcgcag ugacacuacc gucccauucu accagaaagc uccaaacgag gucgcagacu      420

uggcuggagu cacgggaaua caccaagcac cuuauucgcg uugagaacug gaucuuucga      480

aaucccggau ucgcccuggc ugccgcggca auagccuggu ugcucggcag caguacaucc      540

cagaaaguga ucuaucuggu caugauucug cucaucgcuc cugccuacuc uauuagaugc      600

aucggcguga gcaacaggga uuuuguagaa gggauguccg gagguacuug gguugacaua      660

guguuagagc auggcgggug ugucaccgug auggcacagg auaagccaac aguggacauu      720

gagcugguca ccacgacugu uucaaauaug gcugaagugc ggaguuacug cuaugaggcc      780

agcaucucug auauggcguc cgacucacgu ugucccacac aaggagaagc cuaccuugac      840

aaacagagcg auacccagua uguaugcaag cgcacuuugg uggacagagg cugggguaac      900

ggguguggac uguucggcaa aggcagucua gucacaugcg caaaguuugc uugcucuaag      960

aaaaugaccg ggaaguccau ucagccugag aaucucgaau acaggaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugaucguu aacgauacgg gccaugagac cgacgagaau     1080

cgggccaaag uggaaauuac accaaacuca ccccgagcug aggccacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucgc accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga auaacaagca cuggcugguc cauaaagagu gguuccacga cauaccacuc     1260

ccguggcacg caggcgccga uacuggaaca ccccauugga auaacaagga agcucuugug     1320

gaguuuaaag acgcgcacgc aaagagacag accguaguug ugcugggcag ccaggagggg     1380

gccguccaua ccgcuuuggc cggagcacua gaagccgaga uggauggugc uaagggcagg     1440

cuguccucug ggcaccucaa augccggcug aagauggaca aacuucgccu gaagggagug     1500

agcuauucau uguguacugc cgcauucacg uuuacaaaaa ucccugcgga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcu gguacagacg ggccaugcaa ggugcccgcc     1620

caaauggcag uagauaugca gacccugacu ccugucggca gauuaauuac agccaaccca     1680

gugaucacgg aaaguaccga aaauuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uaguuauugg cgugggggag aaaaagauca cucaccacug gcauaggucu     1800

ggaucaacaa ucgguaaggc uuuugaggcc accguccgug gcgcaaaacg gauggcugug     1860

cucggggaca cagccuggga uuucggaagu guaggcggug cgcugaacag ccuugggaaa     1920

ggcauucacc agaucuucgg agccgcuuuu aaguccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugauaggaac ucuccugaug uggcuuggcc ugaauaccaa gaacgguagc     2040

aucuccuuga ugugucucgc auuaggggga gugcugauuu uccugucaac ggccguuagu     2100

gcugacgucg gcugcucugu ggauuuuagc aaa                                  2133


<210>  1662
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1662
aucaacgcca gaaaggagaa aaagaggcgg ggcgcugaca ccagcguggg gauugucgga       60

cugcuccuua caacugcaau ggccgcggaa guuacgcgcc gagguuccgc uuacuauaug      120

uaccuggauc guaaugacgc cggcgaggca auaucuuucc ccaccacauu ggggaugaac      180

aaaugcuaua uccagauuau ggauuuagga cacaugugug acgccacuau gucauacgaa      240

ugcccuaugc uggaugaggg cguggaacca gacgauguag acuguuggug caauaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaagaaag gggaggcuag aaggagccgg      360

cgcgcaguga cacuaccguc ccauucuacc agaaagcucc aaacgagguc gcagacuugg      420

cuggagucac gggaauacac caagcaccuu auccgcguug agaacuggau uuuucgaaau      480

cccggauucg cccuggcugc cgcggcaauc gccugguugc ucggcagcag uacaucccag      540

aaagugauau aucuggucau gauccugcuc auugcuccug ccuacucuau cagaugcauu      600

ggcgugagca acagggauuu uguagaaggg auguccggag guacuugggu ugacaucgug      660

uuagagcaug gcgggugugu caccgugaug gcacaggaua agccaacagu ggacauagag      720

cuggucacca cgacuguuuc aaauauggcu gaagugcgga guuacugcua ugaggccagc      780

auuucugaua uggcguccga cucacguugu cccacacaag gagaagccua ccuugacaaa      840

cagagcgaua cccaguaugu augcaagcgc acuuuggugg acagaggcug ggguaacggg      900

uguggacugu ucggcaaagg cagucuaguc acaugcgcaa aguuugcuug cucuaagaaa      960

augaccggga aguccaucca gccugagaau cucgaauaca ggauuaugcu gagcgugcac     1020

ggaucgcaac acucugguau gaucguuaac gauacgggcc augagaccga cgagaaucgg     1080

gccaaagugg aaaucacacc aaacucaccc cgagcugagg ccacucuugg gggauucggc     1140

ucccuggguu uggauuguga accucgcacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaua acaagcacug gcugguccau aaagaguggu uccacgacau uccacucccg     1260

uggcacgcag gcgccgauac uggaacaccc cauuggaaua acaaggaagc ucuuguggag     1320

uuuaaagacg cgcacgcaaa gagacagacc guaguugugc ugggcagcca ggagggggcc     1380

guccauaccg cuuuggccgg agcacuagaa gccgagaugg auggugcuaa gggcaggcug     1440

uccucugggc accucaaaug ccggcugaag auggacaaac uucgccugaa gggagugagc     1500

uauucauugu guacugccgc auucacguuu acaaaaauac cugcggaaac ccuccauggc     1560

acugucaccg uggagguuca guacgcuggu acagacgggc caugcaaggu gcccgcccaa     1620

auggcaguag auaugcagac ccugacuccu gucggcagau uaaucacagc caacccagug     1680

auuacggaaa guaccgaaaa uuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacaucg uuauaggcgu gggggagaaa aagauuacuc accacuggca uaggucugga     1800

ucaacaaucg guaaggcuuu ugaggccacc guccguggcg caaaacggau ggcugugcuc     1860

ggggacacag ccugggauuu cggaagugua ggcggugcgc ugaacagccu ugggaaaggc     1920

auccaccaga uuuucggagc cgcuuuuaag uccuuguucg gcgggauguc uugguuuucg     1980

caaauccuga uuggaacucu ccugaugugg cuuggccuga auaccaagaa cgguagcaua     2040

uccuugaugu gucucgcauu agggggagug cugaucuucc ugucaacggc cguuagugcu     2100

gacgucggcu gcucugugga uuuuagcaaa                                      2130


<210>  1663
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1663
aacgccagaa aggagaaaaa gaggcggggc gcugacacca gcguggggau cgucggacug       60

cuccuuacaa cugcaauggc cgcggaaguu acgcgccgag guuccgcuua cuauauguac      120

cuggaucgua augacgccgg cgaggcaauu ucuuucccca ccacauuggg gaugaacaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg ccacuauguc auacgaaugc      240

ccuaugcugg augagggcgu ggaaccagac gauguagacu guuggugcaa uaccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aggcuagaag gagccggcgc      360

gcagugacac uaccguccca uucuaccaga aagcuccaaa cgaggucgca gacuuggcug      420

gagucacggg aauacaccaa gcaccuuauu cgcguugaga acuggaucuu ucgaaauccc      480

ggauucgccc uggcugccgc ggcaauugcc ugguugcucg gcagcaguac aucccagaaa      540

gugaucuauc uggucaugau acugcucauc gcuccugccu acucuauuag augcaucggc      600

gugagcaaca gggauuuugu agaagggaug uccggaggua cuuggguuga cauuguguua      660

gagcauggcg ggugugucac cgugauggca caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa uauggcugaa gugcggaguu acugcuauga ggccagcaua      780

ucugauaugg cguccgacuc acguuguccc acacaaggag aagccuaccu ugacaaacag      840

agcgauaccc aguauguaug caagcgcacu uugguggaca gaggcugggg uaacgggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgcaaagu uugcuugcuc uaagaaaaug      960

accgggaagu ccauucagcc ugagaaucuc gaauacagga ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaacgau acgggccaug agaccgacga gaaucgggcc     1080

aaaguggaaa ucacaccaaa cucaccccga gcugaggcca cucuuggggg auucggcucc     1140

cuggguuugg auugugaacc ucgcaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaauaaca agcacuggcu gguccauaaa gagugguucc acgacauccc acucccgugg     1260

cacgcaggcg ccgauacugg aacaccccau uggaauaaca aggaagcucu uguggaguuu     1320

aaagacgcgc acgcaaagag acagaccgua guugugcugg gcagccagga gggggccguc     1380

cauaccgcuu uggccggagc acuagaagcc gagauggaug gugcuaaggg caggcugucc     1440

ucugggcacc ucaaaugccg gcugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucauugugua cugccgcauu cacguuuaca aaaauuccug cggaaacccu ccauggcacu     1560

gucaccgugg agguucagua cgcugguaca gacgggccau gcaaggugcc cgcccaaaug     1620

gcaguagaua ugcagacccu gacuccuguc ggcagauuaa uaacagccaa cccagugauc     1680

acggaaagua ccgaaaauuc caagaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaaaaag auaacucacc acuggcauag gucuggauca     1800

acaauuggua aggcuuuuga ggccaccguc cguggcgcaa aacggauggc ugugcucggg     1860

gacacagccu gggauuucgg aaguguaggc ggugcgcuga acagccuugg gaaaggcauc     1920

caccagaucu ucggagccgc uuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaua ccaagaacgg uagcauuucc     2040

uugauguguc ucgcauuagg gggagugcug auauuccugu caacggccgu uagugcugac     2100

gucggcugcu cuguggauuu uagcaaa                                         2127


<210>  1664
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1664
gccagaaagg agaaaaagag gcggggcgcu gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug caauggccgc ggaaguuacg cgccgagguu ccgcuuacua uauguaccug      120

gaucguaacg acgccggcga ggcaauuucu uuccccacca cauuggggau gaauaaaugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcca cuaugucaua cgaaugcccu      240

augcuggaug agggcgugga accagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaag aaaggggagg cuagaaggag ccggcgcgca      360

gugacacuac cgucccauuc uaccagaaag cuccaaacga ggucgcagac uuggcuggag      420

ucacgggaau acaccaagca ccuuauucgc guugagaauu ggaucuuucg aaaccccgga      480

uucgcccugg cugccgcggc aauugccugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcu ccugccuacu cuauuagaug caucggcgug      600

agcaauaggg auuuuguaga agggaugucc ggagguacuu ggguugacau uguguuagag      660

cauggcgggu gugucaccgu gauggcacag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcugaagug cggaguuacu gcuaugaggc cagcauaucu      780

gauauggcgu ccgacucacg uugucccaca caaggagaag ccuaccuuga caaacagagc      840

gauacccagu auguaugcaa gcgcacuuug guggacagag gcugggguaa ugggugugga      900

cuguucggca aaggcagucu agucacaugc gcaaaguuug cuugcucuaa gaaaaugacc      960

gggaagucca uucagccuga gaaccucgaa uacaggauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaga ccgacgagaa ccgggccaaa     1080

guggaaauca caccaaauuc accccgagcu gaggccacuc uugggggauu cggcucccug     1140

gguuuggauu gugaaccucg caccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaagc acuggcuggu ccauaaagag ugguuccacg acaucccacu cccguggcac     1260

gcaggcgccg auacuggaac accccauugg aacaauaagg aagcucuugu ggaguuuaaa     1320

gacgcgcacg caaagagaca gaccguaguu gugcugggca gccaggaggg ggccguccau     1380

accgcuuugg ccggagcacu agaagccgag auggauggug cuaagggcag gcuguccucu     1440

gggcaccuca aaugccggcu gaagauggac aaacuucgcc ugaagggagu gagcuauuca     1500

uuguguacug ccgcauucac guuuacaaaa auuccugcgg aaacccucca uggcacuguc     1560

accguggagg uucaguacgc ugguacagac gggccaugca aggugcccgc ccaaauggca     1620

guagauaugc agacccugac uccugucggc agauuaauaa cagccaaccc agugaucacg     1680

gaaaguaccg agaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagguc uggaucaaca     1800

auugguaagg cuuuugaagc caccguccgu ggcgcaaaac ggauggcugu gcucggggac     1860

acagccuggg auuucggaag uguaggcggu gcgcugaaua gccuugggaa aggcauccac     1920

cagaucuucg gagccgcuuu uaaguccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cauuaggggg agugcugaua uuccugucaa cggccguuag ugcugacguc     2100

ggcugcucug uggauuuuag caaa                                            2124


<210>  1665
<211>  2121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1665
agaaaggaga aaaagaggcg gggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga aguuacgcgc cgagguuccg cguacuauau guaccuggau      120

cguaacgacg cuggcgaggc cauuucuuuc cccaccacau uggggaugaa uaaaugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaagaaa ggggaggcca gaaggagccg gcgcgcugug      360

acacuaccgu cccauucuac cagaaagcuc caaacgaggu cgcagacuug gcuggaguca      420

cgggaauaca ccaagcaccu uauucgcguu gagaauugga ucuuucgaaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uuagaugcau cggcgugagc      600

aauagggauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaagugcgg aguuacugcu augaggcuag cauaucugau      780

auggccuccg acucacguug ucccacacaa ggagaagcgu accuugacaa acagagcgau      840

acccaguaug uaugcaagcg cacuuuggug gacagaggcu gggguaaugg guguggacug      900

uucggcaaag gcagucuagu cacaugcgcc aaguuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac aggaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgagaaccg ggcuaaagug     1080

gaaaucacac caaauucacc ccgagccgag gcuacucuug ggggauucgg cucccugggu     1140

uuggauugug aaccucgcac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaagcacu ggcuggucca uaaagagugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaggaag cccuugugga guuuaaagac     1320

gcucacgcga agagacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga agcagagaug gauggugcca agggcaggcu guccucuggg     1440

caccucaaau gccggcugaa gauggacaaa cuucgccuga agggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaaaauu ccugcagaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcggg uacagacggg ccaugcaagg ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggcaga uuaauaacag caaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc auaggucugg aucaacaauu     1800

gguaaagccu uugaagcuac cguccguggc gccaagcgga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaagg cauccaccag     1920

aucuucggag cggccuuuaa guccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuu uagggggagu gcugauauuc cugucaacgg caguuagugc cgacgucggc     2100

ugcucugugg auuuuagcaa a                                               2121


<210>  1666
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1666
aaggagaaaa agagaagggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaagu uacgcggcgc gguuccgcgu acuauaugua ccuggaucga      120

aacgacgcug gcgaggccau uucuuucccc accacauugg ggaugaauaa augcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgaaug cccuaugcug      240

gaugagggcg uggaaccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caagaaaggg gaggcccgua gaagcaggcg ggcugugaca      360

cuaccguccc auucuacccg caagcuccaa acgagaucgc agacuuggcu ggagucaagg      420

gaauacacca agcaccuuau ucggguugag aauuggaucu uucgcaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc gaugcaucgg cgugagcaau      600

agagauuuug uagaagggau guccggaggu acuuggguug acauuguguu agagcauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga agugaggagu uacugcuaug aggcuagcau aucugauaug      780

gccuccgacu cacggugucc cacacaagga gaagcguacc uugacaaaca gagcgauacc      840

caguauguau gcaagcguac uuugguggac cgcggcuggg guaaugggug uggacuguuc      900

ggcaaaggca gucuagucac augcgccaag uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugagaaccu cgaauacaga aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gagaccgacg agaacagggc uaaaguggaa     1080

aucacaccaa auucaccccg ggccgaggcu acucuugggg gauucggcuc ccuggguuug     1140

gauugugaac cucgaaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aagcacuggc ugguccauaa agagugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaggaagccc uuguggaguu uaaagacgcu     1320

cacgcgaagc gccagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaagc agagauggau ggugccaagg gcagacuguc cucugggcac     1440

cucaaaugca ggcugaagau ggacaaacuu cggcugaagg gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaaaauuccu gcagaaaccc uccauggcac ugucaccgug     1560

gagguucagu acgcggguac agacgggcca ugcaaggugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggccgcuua auaacagcaa acccagugau cacggaaagu     1680

accgagaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcaua gaucuggauc aacaauuggu     1800

aaagccuuug aagcuaccgu caggggcgcc aagcguaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaaaggcau ccaccagauc     1920

uucggagcgg ccuuuaaguc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcuuuag ggggagugcu gauauuccug ucaacggcag uuagugccga cgucggcugc     2100

ucuguggauu uuagcaaa                                                   2118


<210>  1667
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1667
gagaagaaaa gaaggggcgc cgacaccagc guggggaucg ucggacugcu ccuuacaacu       60

gcuauggcag ccgaaguuac gcggcgcggu uccgcguacu auauguaccu ggaucgaaac      120

gacgcuggcg aggccauuuc uuuccccacc acauugggga ugaauaagug cuauauacag      180

aucauggauu uaggacacau gugugacgca acuaugucau acgaaugccc uaugcuggau      240

gagggcgugg aaccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uaugguacuu gucaucacaa aaagggggag gcccguagaa gcaggcgggc ugugacacua      360

ccgucccauu cuacccgcaa acuccaaacg agaucgcaga cuuggcugga gucaagggaa      420

uacaccaagc accuuauucg gguugagaau uggaucuuuc gcaaccccgg auucgcacug      480

gccgcugccg cgauugcaug guugcucggc agcaguacau cccagaaggu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucgau gcaucggcgu gagcaauaga      600

gauuuuguag aagggauguc cggagguacu uggguugaca uuguguuaga gcauggcggg      660

ugugucaccg ugauggccca ggauaaacca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaagu gaggaguuac ugcuaugagg cuagcauauc ugauauggcc      780

uccgacucac ggugucccac acaaggagaa gcguaccuug acaagcagag cgauacccag      840

uauguaugca aacguacuuu gguggaccgc ggcuggggua augggugugg acuguucggc      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaagaugac cgggaaaucc      960

auucagccug agaaccucga auacagaauc augcugagcg ugcacggauc gcaacacucu     1020

gguaugauug uuaaugauac gggccaugag accgacgaga acagggcuaa gguggaaauc     1080

acaccaaauu caccccgggc cgaggcuacu cuugggggau ucggcucccu ggguuuggau     1140

ugugaaccuc gaaccgggcu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga gugguuccac gacaucccac ucccguggca cgccggcgca     1260

gauacuggaa caccccauug gaacaauaaa gaagcccuug uggaguuuaa ggacgcucac     1320

gcgaaacgcc agaccguagu ugugcugggc agccaggagg gggcagucca uaccgccuug     1380

gcuggagccc uagaagcaga gauggauggu gccaagggca gacuguccuc ugggcaccuc     1440

aagugcaggc ugaaaaugga caagcugcgg cugaaaggag ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gaaacccucc auggcacugu caccguggag     1560

guucaguacg cggguacaga cgggccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg ccgcuuaaua acagcaaacc cagugaucac ggaaaguacc     1680

gagaacucca agaugaugcu ggagcuagau cccccguucg gagacagcua cauuguuauc     1740

ggcguggggg aaaagaaaau aacucaccac uggcauagau cuggaucaac aauugguaag     1800

gccuuugagg cuaccgucag gggcgccaaa cguauggcag ugcucgggga cacagcuugg     1860

gauuucggaa guguaggcgg ugcccugaau agccuuggga agggcaucca ccagaucuuc     1920

ggagcggccu uuaaauccuu guucggcggg augucuuggu uuucgcaaau ucugaucgga     1980

acucuccuga uguggcuugg ccugaacacc aagaauggua gcauuuccuu gaugugucuc     2040

gcucuugggg gaguguuaau auuccuguca acggcaguua gugccgacgu cggcugcucu     2100

guggauuuua gcaag                                                      2115


<210>  1668
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1668
aagaaaagaa ggggcgccga caccagcgug gggaucgucg gacugcuccu uacaacugcu       60

auggcagccg agguuacgcg gcgcgguucc gcguacuaua uguaccugga ucgaaacgac      120

gcuggcgaag ccauuucuuu ccccaccaca uuggggauga auaagugcua uauacagauc      180

auggauuuag gacacaugug ugacgcaacu augucauacg agugcccuau gcuggaugaa      240

ggcguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

gguacuuguc aucacaaaaa gggggaagcc cguagaagca ggcgggcugu gacacuaccg      360

ucccauucua cccgcaaacu ccaaacgaga ucgcagacuu ggcuggaguc aagggaguac      420

accaagcacc uuauucgggu ugaaaauugg aucuuucgca accccggauu cgcacuggcc      480

gcugccgcga uugcaugguu gcucggcagc aguacauccc agaaggugau cuaucugguc      540

augauacugc ucaucgcccc ugcuuacucu auucgaugca ucggcgugag caauagagau      600

uuuguagagg ggauguccgg agguacuugg guugacauug uguuagaaca uggcgggugu      660

gucaccguga uggcccagga uaaaccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg cagaggugag gaguuacugc uaugaagcua gcauaucuga uauggccucc      780

gacucacggu gucccacaca aggagaggcg uaccuugaca agcagagcga uacccaguau      840

guaugcaaac guacuuuggu ggaccgcggc ugggguaaug gguguggacu guucggcaag      900

ggcagucuag ucacaugcgc caaauuugca ugcucuaaga agaugaccgg gaaauccauu      960

cagccugaaa accucgagua cagaaucaug cugagcgugc acggaucgca acacucuggu     1020

augauuguua augauacggg ccaugaaacc gacgagaaca gggcuaaggu ggagaucaca     1080

ccaaauucac cccgggccga agcuacucuu gggggauucg gcucccuggg uuuggauugu     1140

gagccucgaa ccgggcucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc cggcgcagau     1260

acuggaacac cccauuggaa caauaaagag gcccuugugg aauuuaagga cgcucacgcg     1320

aaacgccaga ccguaguugu gcugggcagc caggaggggg caguccauac cgccuuggcu     1380

ggagcccuag aggcagaaau ggauggugcc aagggcagac uguccucugg gcaccucaag     1440

ugcaggcuga aaauggacaa gcugcggcug aaaggaguga gcuauucauu guguacugcu     1500

gccuucacgu uuacaaagau uccugcagag acccuccaug gcacugucac cguggaaguu     1560

caguacgcgg guacagacgg gccaugcaaa gugcccgcuc aaauggccgu agauaugcag     1620

acccugacuc cugucggccg cuuaauaaca gcaaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggag acagcuacau uguuaucggc     1740

gugggggaga agaaaauaac ucaccacugg cauagaucug gaucaacaau ugguaaggcc     1800

uuugaagcua ccgucagggg cgccaaacgu auggcagugc ucggggacac agcuugggau     1860

uucggaagug uaggcggugc ccugaauagc cuugggaagg gcauccacca gaucuucgga     1920

gcggccuuua aauccuuguu cggcgggaug ucuugguuuu cgcaaauucu gaucggaacu     1980

cuccugaugu ggcuuggccu gaacaccaag aaugguagca uuuccuugau gugucucgcu     2040

cuugggggag uguuaauauu ccugucaacg gcaguuagug ccgacgucgg cugcucugug     2100

gauuuuagca ag                                                         2112


<210>  1669
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1669
aagagaaggg gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug       60

gcagccgagg uuacgcggcg cgguuccgcg uacuauaugu accuggaucg aaacgacgcu      120

ggcgaagcca uuucuuuccc caccacauug gggaugaaua aaugcuauau acagaucaug      180

gauuuaggac acauguguga cgcaacuaug ucauacgagu gcccuaugcu ggaugaaggc      240

guggagccag acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu      300

acuugucauc acaagaaagg ggaagcccgu agaagcaggc gggcugugac acuaccgucc      360

cauucuaccc gcaagcucca aacgagaucg cagacuuggc uggagucaag ggaguacacc      420

aaacaccuua uucggguuga aaauuggauc uuucgcaacc ccggauucgc acuggccgcu      480

gccgcgauug caugguugcu cggcagcagu acaucccaga aggugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cgaugcaucg gcgugagcaa uagagauuuu      600

guagagggga uguccggagg uacuuggguu gacauugugu uagaacaugg cggguguguc      660

accgugaugg cccaggauaa gccaacagug gacaucgagc uggucaccac gacuguuuca      720

aacauggcag aggugaggag uuacugcuau gaagcuagca uaucugauau ggccuccgac      780

ucacgguguc ccacacaagg agaggcguac cuugacaaac agagcgauac ccaguaugua      840

ugcaagcgua cuuuggugga ccgcggcugg gguaaugggu guggacuguu cggcaaaggc      900

agucuaguca caugcgccaa guuugcaugc ucuaaaaaga ugaccgggaa guccauucag      960

ccugaaaacc ucgaguacag aaucaugcug agcgugcacg gaucgcaaca cucugguaug     1020

auuguuaaug auacgggcca ugaaaccgac gagaacaggg cuaaagugga gaucacacca     1080

aauucacccc gggccgaagc uacucuuggg ggauucggcu cccuggguuu ggauugugag     1140

ccucgaaccg ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaagcacugg     1200

cugguccaua aagaaugguu ccacgacauc ccacucccgu ggcacgccgg cgcagauacu     1260

ggaacacccc auuggaacaa uaaggaggcc cuuguggaau uuaaagacgc ucacgcgaag     1320

cgccagaccg uaguugugcu gggcagccag gagggggcag uccauaccgc cuuggcugga     1380

gcccuagagg cagaaaugga uggugccaaa ggcagacugu ccucugggca ccucaagugc     1440

aggcugaaga uggacaaacu ucggcugaag ggagugagcu auucauugug uacugcugcc     1500

uucacguuua caaaaauucc ugcagagacc cuccauggca cugucaccgu ggaaguucag     1560

uacgcgggua cagacgggcc augcaaggug cccgcucaaa uggccguaga uaugcagacc     1620

cugacuccug ucggccgcuu aauaacagca aacccaguga ucacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggagaca gcuacauugu uaucggcgug     1740

ggggagaaga agauaacuca ccacuggcau agaucuggau caacaauugg uaaagccuuu     1800

gaagcuaccg ucaggggcgc caagcguaug gcagugcucg gggacacagc uugggauuuc     1860

ggaaguguag gcggugcccu gaauagccuu gggaaaggca uccaccagau cuucggagcg     1920

gccuuuaagu ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc     1980

cugauguggc uuggccugaa caccaaaaau gguagcauuu ccuugaugug ucucgcuuua     2040

gggggagugc ugauauuccu gucaacggca guuagugccg acgucggcug cucuguggau     2100

uuuagcaag                                                             2109


<210>  1670
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1670
agaaggggcg ccgacaccag cguggggauc gucggacugc uccuuacaac ugcuauggca       60

gccgagguua cgcggcgcgg uuccgcguac uauauguacc uggaucgaaa cgacgcuggc      120

gaagccauuu cuuuccccac cacauugggg augaauaagu gcuauauaca gaucauggau      180

uuaggacaca ugugugacgc aacuauguca uacgagugcc cuaugcugga ugaaggcgug      240

gagccagacg auguagacug uuggugcaac accacaagua ccuggguggu cuaugguacu      300

ugucaucaca aaaaggggga agcccguaga agcaggcggg cugugacacu accgucccau      360

ucuacccgca aacuccaaac gagaucgcag acuuggcugg agucaaggga guacaccaag      420

caccuuauuc ggguugaaaa uuggaucuuu cgcaaccccg gauucgcacu ggccgcugcc      480

gcgauugcau gguugcucgg cagcaguaca ucccagaaag ugaucuaucu ggucaugaua      540

cugcucaucg ccccugcuua cucuauucga ugcaucggcg ugagcaauag agauuuugua      600

gaggggaugu ccggagguac uuggguugac auuguguuag aacauggcgg gugugucacc      660

gugauggccc aggauaagcc aacaguggac aucgagcugg ucaccacgac uguuucaaac      720

auggcagagg ugaggaguua cugcuaugaa gcuagcauau cugauauggc cuccgacuca      780

cgguguccca cacaaggaga ggcguaccuu gacaagcaga gcgauaccca guauguaugc      840

aaacguacuu ugguggaccg cggcuggggu aaugggugug gacuguucgg caagggcagu      900

cuagucacau gcgccaaauu ugcaugcucu aagaaaauga ccgggaaguc cauucagccu      960

gaaaaccucg aguacagaau caugcugagc gugcacggau cgcaacacuc ugguaugauu     1020

guuaaugaua cgggccauga aaccgacgag aacagggcua agguggagau cacaccaaau     1080

ucaccccggg ccgaagcuac ucuuggggga uucggcuccc uggguuugga uugugagccu     1140

cgaaccgggc ucgacuuuag ugaucuguau uacuuaacaa ugaacaauaa acacuggcug     1200

guccauaagg aaugguucca cgacauccca cucccguggc acgccggcgc agauacugga     1260

acaccccauu ggaacaauaa agaggcccuu guggaauuua aggacgcuca cgcgaaacgc     1320

cagaccguag uugugcuggg cagccaggag ggggcagucc auaccgccuu ggcuggagcc     1380

cuagaggcag aaauggaugg ugccaagggc agacuguccu cugggcaccu caaaugcagg     1440

cugaagaugg acaagcugcg gcugaaagga gugagcuauu cauuguguac ugcugccuuc     1500

acguuuacaa agauuccugc agagacccuc cauggcacug ucaccgugga aguucaguac     1560

gcggguacag acgggccaug caaagugccc gcucaaaugg ccguagauau gcagacccug     1620

acuccugucg gccgcuuaau aacagcaaac ccagugauca cggagaguac cgaaaacucc     1680

aagaugaugc uggagcuaga ucccccguuc ggagacagcu acauuguuau cggcgugggg     1740

gagaaaaaga uaacucacca cuggcauaga ucuggaucaa caauugguaa ggccuuugaa     1800

gcuaccguca ggggcgccaa acguauggca gugcucgggg acacagcuug ggauuucgga     1860

aguguaggcg gugcccugaa uagccuuggg aagggcaucc accagaucuu cggagcggcc     1920

uuuaaauccu uguucggcgg gaugucuugg uuuucgcaaa uucugaucgg aacucuccug     1980

auguggcuug gccugaacac caagaauggu agcauuuccu ugaugugucu cgcucuuggg     2040

ggaguguuaa uauuccuguc aacggcaguu agugccgacg ucggcugcuc uguggauuuu     2100

agcaaa                                                                2106


<210>  1671
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1671
agaggcgccg acaccagcgu ggggaucguc ggacugcucc uuacaacugc uauggcagcc       60

gagguuacga ggcgggguuc cgcguacuau auguaccugg aucgcaacga cgcuggcgaa      120

gccauuucuu uccccaccac auuggggaug aauaagugcu auauacagau cauggauuua      180

ggacacaugu gugacgcaac uaugucauac gagugcccua ugcuggauga aggcguggag      240

ccagacgaug uagacuguug gugcaacacc acaaguaccu ggguggucua ugguacuugu      300

caucacaaaa agggggaagc ccgacguagc agaagggcug ugacacuacc gucccauucu      360

acccggaaac uccaaacgcg cucgcagacu uggcuggagu caagagagua caccaagcac      420

cuuauuaggg uugaaaauug gaucuuucgg aaccccggau ucgcacuggc cgcugccgcg      480

auugcauggu ugcucggcag caguacaucc cagaaaguga ucuaucuggu caugauacug      540

cucaucgccc cugcuuacuc uauucgcugc aucggcguga gcaaucgaga uuuuguagag      600

gggauguccg gagguacuug gguugacauu guguuagaac auggcgggug ugucaccgug      660

auggcccagg auaagccaac aguggacauc gagcugguca ccacgacugu uucaaacaug      720

gcagagguga gaaguuacug cuaugaagcu agcauaucug auauggccuc cgacucaagg      780

ugucccacac aaggagaggc guaccuugac aagcagagcg auacccagua uguaugcaaa      840

cggacuuugg uggaccgugg cugggguaau ggguguggac uguucggcaa gggcagucua      900

gucacaugcg ccaaauuugc augcucuaag aaaaugaccg ggaaguccau ucagccugaa      960

aaccucgagu accgcaucau gcugagcgug cacggaucgc aacacucugg uaugauuguu     1020

aaugauacgg gccaugaaac cgacgagaac agagcuaagg uggagaucac accaaauucc     1080

cccagggccg aagcuacucu ugggggauuc ggcucacugg guuuggauug ugagccucgg     1140

accgggcucg acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc     1200

cauaaggaau gguuccacga caucccacuc ccguggcacg ccggcgcaga uacuggaaca     1260

ccccauugga acaauaaaga ggcccuugug gaauuuaagg acgcucacgc gaaacgacag     1320

accguaguug ugcugggcag ccaggagggg gcaguccaua ccgccuuggc uggagcccua     1380

gaggcagaaa uggauggugc caagggccgc cuguccucug ggcaccucaa augcagacug     1440

aagauggaca agcugaggcu gaaaggagug agcuauucau uguguacugc ugccuucacg     1500

uuuacaaaga uuccugcaga gacccuccau ggcacuguca ccguggaagu ucaguacgcg     1560

gguacagacg ggccaugcaa agugcccgcu caaauggccg uagauaugca gacccugacu     1620

ccugucggcc gguuaauaac agcaaaccca gugaucacgg agaguaccga aaacuccaag     1680

augaugcugg agcuagaucc cccguucgga gacagcuaca uuguuaucgg cgugggggag     1740

aaaaagauaa cucaccacug gcaucgcucu ggaucaacaa uugguaaggc cuuugaagcu     1800

accgucagag gcgccaaaag gauggcagug cucggggaca cagcuuggga uuucggaagu     1860

guaggcggug cccugaauag ccuugggaag ggcauccacc agaucuucgg agcggccuuu     1920

aaauccuugu ucggcgggau gucuugguuu ucgcaaauuc ugaucggaac ucuccugaug     1980

uggcuuggcc ugaacaccaa gaaugguagc auuuccuuga ugugucucgc ucuuggggga     2040

guguuaauau uccugucaac ggcaguuagu gccgacgucg gcugcucugu ggauuuuagc     2100

aaa                                                                   2103


<210>  1672
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1672
ggcgccgaca ccagcguggg gaucgucgga cugcuccuua caacugcuau ggcagccgag       60

guuacgagaa gggguuccgc guacuauaug uaccuggauc ggaacgacgc uggcgaagcc      120

auuucuuucc ccaccacauu ggggaugaau aagugcuaua uacagaucau ggauuuagga      180

cacaugugug acgcaacuau gucauacgag ugcccuaugc uggaugaagg cguggagcca      240

gacgauguag acuguuggug caacaccaca aguaccuggg uggucuaugg uacuugucau      300

cacaaaaagg gggaagcccg ccgaagccgu agagcuguga cacuaccguc ccauucuacc      360

aggaaacucc aaacgcgguc gcagacuugg cuggagucac gcgaguacac caagcaccuu      420

auuagaguug aaaauuggau cuuuaggaac cccggauucg cacuggccgc ugccgcgauu      480

gcaugguugc ucggcagcag uacaucccag aaagugaucu aucuggucau gauacugcuc      540

aucgccccug cuuacucuau ucggugcauc ggcgugagca aucgcgauuu uguagagggg      600

auguccggag guacuugggu ugacauugug uuagaacaug gcgggugugu caccgugaug      660

gcccaggaua agccaacagu ggacaucgag cuggucacca cgacuguuuc aaacauggca      720

gaggugcgaa guuacugcua ugaagcuagc auaucugaua uggccuccga cucaagaugu      780

cccacacaag gagaggcgua ccuugacaag cagagcgaua cccaguaugu augcaaaagg      840

acuuuggugg accggggcug ggguaauggg uguggacugu ucggcaaggg cagucuaguc      900

acaugcgcca aauuugcaug cucuaagaaa augaccggga aguccauuca gccugaaaac      960

cucgaguacc guaucaugcu gagcgugcac ggaucgcaac acucugguau gauuguuaau     1020

gauacgggcc augaaaccga cgagaaccgc gcuaaggugg agaucacacc aaauuccccc     1080

agagccgaag cuacucuugg gggauucggc ucacuggguu uggauuguga gccuaggacc     1140

gggcucgacu uuagugaucu guauuacuua acaaugaaca auaaacacug gcugguccau     1200

aaggaauggu uccacgacau cccacucccg uggcacgccg gcgcagauac uggaacaccc     1260

cauuggaaca auaaagaggc ccuuguggaa uuuaaggacg cucacgcgaa acggcagacc     1320

guaguugugc ugggcagcca ggagggggca guccauaccg ccuuggcugg agcccuagag     1380

gcagaaaugg auggugccaa gggccgacug uccucugggc accucaaaug ccgccugaag     1440

auggacaagc ugagacugaa aggagugagc uauucauugu guacugcugc cuucacguuu     1500

acaaagauuc cugcagagac ccuccauggc acugucaccg uggaaguuca guacgcgggu     1560

acagacgggc caugcaaagu gcccgcucaa auggccguag auaugcagac ccugacuccu     1620

gucggcaggu uaauaacagc aaacccagug aucacggaga guaccgaaaa cuccaagaug     1680

augcuggagc uagauccccc guucggagac agcuacauug uuaucggcgu gggggagaaa     1740

aagauaacuc accacuggca ucggucugga ucaacaauug guaaggccuu ugaagcuacc     1800

guccgcggcg ccaaaagaau ggcagugcuc ggggacacag cuugggauuu cggaagugua     1860

ggcggugccc ugaauagccu ugggaagggc auccaccaga ucuucggagc ggccuuuaaa     1920

uccuuguucg gcgggauguc uugguuuucg caaauucuga ucggaacucu ccugaugugg     1980

cuuggccuga acaccaagaa ugguagcauu uccuugaugu gucucgcucu ugggggagug     2040

uuaauauucc ugucaacggc aguuagugcc gacgucggcu gcucugugga uuuuagcaaa     2100


<210>  1673
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1673
gccgacacca gcgugggcau cgucgggcug cuccuuacaa cugcuauggc agccgagguu       60

acgagaaggg gauccgcgua cuauauguac cuggaucgga acgacgcugg ugaagccauu      120

ucuuucccca ccacauuggg caugaauaag ugcuauauac agaucaugga uuuagggcac      180

augugugacg caacuauguc auacgagugc ccuaugcugg augaaggagu ggagccagac      240

gauguagacu guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac      300

aaaaagggug aagcccgccg aagccguaga gcugugacac uaccguccca uucuaccagg      360

aaacuccaaa cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu      420

agaguugaaa auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca      480

ugguugcucg gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc      540

gccccugcuu acucuauucg gugcaucggc gugagcaauc gcgauuuugu agagggcaug      600

uccgggggaa cuuggguuga cauuguguua gaacauggug gcugugucac cgugauggcc      660

caggauaagc caacagugga caucgagcug gucaccacga cuguuucaaa cauggcagag      720

gugcgaaguu acugcuauga agcuagcaua ucugauaugg ccuccgacuc aagauguccc      780

acacaagggg aggcguaccu ugacaagcag agcgauaccc aguauguaug caaaaggacu      840

uugguggacc ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca      900

ugcgccaaau uugcaugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc      960

gaguaccgua ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugau     1020

acgggucaug aaaccgacga gaaccgcgcu aagguggaga ucacaccaaa uucccccaga     1080

gccgaagcua cucuuggcgg guucggauca cugggcuugg auugugagcc uaggaccggu     1140

cucgacuuua gugaucugua uuacuuaaca augaacaaua aacacuggcu gguccauaag     1200

gaaugguucc acgacauccc acucccgugg cacgccgggg cagauacugg cacaccccau     1260

uggaacaaua aagaggcccu uguggaauuu aaggacgcuc acgcgaaacg gcagaccgua     1320

guugugcugg gaagccagga gggcgcaguc cauaccgccu uggcuggggc ccuagaggca     1380

gaaauggaug gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug     1440

gacaaacuua gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca     1500

aagauuccug cagagacccu ccauggaacu gucaccgugg aaguucagua cgcgggcaca     1560

gacgguccau gcaaagugcc cgcucaaaug gccguagaua ugcagacccu gacuccuguc     1620

gggagguuaa uaacagcaaa cccagugauc acggagagua ccgaaaacuc caagaugaug     1680

cuggagcuag aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag     1740

auaacucacc acuggcaucg gucuggguca acaauuggaa aggccuuuga agcuaccguc     1800

cgcggugcca aaagaauggc agugcucggc gacacagcuu gggauuucgg gaguguagga     1860

ggcgcccuga auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc     1920

uuguucggag gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu     1980

ggacugaaca ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua     2040

auauuccugu caacggcagu uagugccgac gucggaugcu cuguggauuu uagcaaa        2097


<210>  1674
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1674
gacaccagcg ugggcaucgu cgggcugcuc cuuacaacug ccauggcugc agagguuacg       60

agaaggggau ccgccuacua uauguaccug gaucggaacg acgcggguga agcuauuucu      120

uuccccacca cauugggcau gaauaagugc uauauacaga ucauggauuu agggcacaug      180

ugugacgcca cuaugucaua cgagugcccu augcuggaug aaggagugga gccagacgau      240

guagacuguu ggugcaacac cacaaguacc uggguggucu auggcacuug ucaucacaaa      300

aagggugaag cacgccgaag ccguagagcc gugacacuac cgucccauuc uaccaggaaa      360

cuccaaacgc ggucgcagac uuggcuggag ucacgcgagu acaccaagca ccuuauuaga      420

guugaaaauu ggaucuuuag gaaccccggg uucgcucugg cagccgcugc cauugcgugg      480

uugcucggaa gcaguacauc ccagaaagug aucuaucugg ucaugauacu gcucaucgca      540

cccgccuacu cuauucggug caucggcgug agcaaucgcg auuuuguaga gggcaugucc      600

gggggaacuu ggguugacau uguguuagaa caugguggcu gugucaccgu gauggcucag      660

gauaagccaa caguggacau cgagcugguc accacgacug uuucaaacau ggccgaggug      720

cgaaguuacu gcuaugaagc aagcauaucu gauauggcuu ccgacucaag augucccaca      780

caaggggagg ccuaccuuga caagcagagc gauacccagu auguaugcaa aaggacuuug      840

guggaccggg gauggggcaa ugguuguggg cuguucggaa agggcagucu agucacaugc      900

gcgaaauuug ccugcucuaa gaaaaugacc ggcaagucca uucagccuga aaaccucgag      960

uaccguauca ugcugagcgu gcacgggucg caacacucug gaaugauugu uaaugauacg     1020

ggucaugaaa ccgacgagaa ccgcgcaaag guggagauca caccaaauuc ccccagagcu     1080

gaagccacuc uuggcggguu cggaucacug ggcuuggauu gugagccuag gaccggucuc     1140

gacuuuagug aucuguauua cuuaacaaug aacaauaaac acuggcuggu ccauaaggaa     1200

ugguuccacg acaucccacu cccguggcac gcuggggccg auacuggcac cccucauugg     1260

aacaauaaag aggcacuugu ggaauuuaag gacgcccacg cuaaacggca gacaguaguu     1320

gugcugggaa gccaggaggg cgcgguccau accgcauugg ccggggcucu agaggccgaa     1380

auggauggag caaagggucg acuguccucu ggccaccuca aaugccgccu gaagauggac     1440

aaacuuagac ugaagggggu gagcuauuca uuguguacug ccgcuuucac guuuacaaag     1500

auuccugccg agacccucca uggaacuguc accguggaag uucaguacgc aggcacagac     1560

gguccaugca aagugcccgc gcaaauggcu guagauaugc agacccugac uccugucggg     1620

agguuaauaa cagccaaccc agugaucacg gagaguaccg aaaacuccaa gaugaugcug     1680

gagcuagauc ccccguucgg cgacagcuac auuguuaucg gagugggcga gaaaaagaua     1740

acucaccacu ggcaucgguc ugggucaaca auuggaaagg cauuugaagc caccguccgc     1800

ggugcuaaaa gaauggccgu gcucggcgac acagcauggg auuucgggag uguaggaggc     1860

gcucugaaua gccuugguaa ggggauccac cagaucuucg gcgccgcguu uaaauccuug     1920

uucggaggca ugucuugguu uucgcaaauu cugaucggga cucuccugau guggcuugga     1980

cugaacacca agaauggcag cauuuccuug augugucucg cccugggugg gguguuaaua     2040

uuccugucaa cggcuguuag ugcagacguc ggaugcucug uggauuuuag caaa           2094


<210>  1675
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1675
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc agaugucgga ugcucugugg acuuuagcaa a              2091


<210>  1676
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1676
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcagc cguuacgaga       60

aggggauccg cguacuauau guaccuggac cggaacgaug cuggugaggc cauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugcaacua ugucauacga augcccuaug cuggacgagg gaguggaacc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaggccc gccgaagccg uagagcugug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaauca cgcgaguaca ccaagcaccu uauuagaguu      420

gagaauugga ucuuuaggaa ccccggguuc gcacuggccg cugccgcgau ugcaugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcuuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaagg cauguccggg      600

ggaacuuggg uugauauugu guuagagcau gguggcugug ucaccgugau ggcccaggac      660

aagccaacag uggauaucga acuggucacc acgacuguuu caaacauggc agaggugcga      720

aguuacugcu augaggcuag cauaucugac auggccuccg auucaagaug ucccacacaa      780

ggggaagcgu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcc      900

aaauuugcau gcucuaagaa aaugaccggc aaguccauuc agccugagaa ccucgaauac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugagaccg augaaaaccg cgcuaaggug gagaucacac caaauucccc cagagccgag     1080

gcuacucuug gcggguucgg aucacugggc uuggacugug aaccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggagugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcagaca cuggcacacc ccauuggaac     1260

aauaaagaag cccuugugga guuuaaggau gcucacgcga aacggcagac cguaguugug     1320

cugggaagcc aggaaggcgc aguccauacc gccuuggcug gggcccuaga ggcagagaug     1380

gacggagcca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugcug ccuucacguu uacaaagauu     1500

ccugcagaaa cccuccaugg aacugucacc guggagguuc aguacgcggg cacagacggu     1560

ccaugcaaag ugcccgcuca aauggccgua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag caaacccagu gaucacggaa aguaccgaga acuccaagau gaugcuggaa     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggccu uugaggcuac cguccgcggu     1800

gccaaaagaa uggcagugcu cggcgauaca gcuugggacu ucgggagugu aggaggcgcc     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg cggccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgcuc uggguggggu guuaauauuc     2040

cugucaacgg caguuagugc cgaugucgga ugcucugugg acuuuagcaa a              2091


<210>  1677
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1677
agaggcgccg acaccagcgu ggggaucguc ggacugcucc uuacaacugc uauggcagcc       60

gcgguuacga ggcgggguuc cgcuuacuau auguaccugg aucgcaacga cgccggcgag      120

gcaauuucuu uccccaccac auuggggaug aauaagugcu auauacagau cauggauuua      180

ggacacaugu gugacgccac uaugucauac gaaugcccua ugcuggauga gggcguggaa      240

ccagacgaug uagacuguug gugcaacacc acaaguaccu ggguggucua ugguacuugu      300

caucacaaaa agggggaggc ucgacguagc agaagggcag ugacacuacc gucccauucu      360

acccggaaac uccaaacgcg cucgcagacu uggcuggaau caagagagua caccaagcac      420

cuuauuaggg uugagaauug gaucuuucgg aaccccggau ucgcccuggc ugccgcggca      480

auugccuggu ugcucggcag caguacaucc cagaaaguga ucuaucuggu caugauacug      540

cucaucgcuc cugccuacuc uauucgcugc aucggcguga gcaaucgaga uuuuguagaa      600

gggauguccg gagguacuug gguugacauu guguuagagc auggcgggug ugucaccgug      660

auggcacagg auaagccaac aguggacauc gaacugguca ccacgacugu uucaaacaug      720

gcugagguga gaaguuacug cuaugaggcc agcauaucug auauggcguc cgacucaagg      780

ugucccacac aaggagaagc cuaccuugac aagcagagcg auacccagua uguaugcaaa      840

cggacuuugg uggaccgugg cugggguaau ggguguggac uguucggcaa gggcagucua      900

gucacaugcg caaaauuugc uugcucuaag aaaaugaccg ggaaguccau ucagccugag      960

aaccucgaau accgcaucau gcugagcgug cacggaucgc aacacucugg uaugauuguu     1020

aaugauacgg gccaugagac cgacgaaaac agagccaagg uggagaucac accaaauucc     1080

cccagggcug aggccacucu ugggggauuc ggcucacugg guuuggauug ugaaccucgg     1140

accgggcucg acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc     1200

cauaaggagu gguuccacga caucccacuc ccguggcacg caggcgccga uacuggaaca     1260

ccccauugga acaauaaaga agcucuugug gaguuuaagg acgcgcacgc aaaacgacag     1320

accguaguug ugcugggcag ccaggaaggg gccguccaua ccgcuuuggc cggagcacua     1380

gaggccgaga uggauggugc uaagggccgc cuguccucug ggcaccucaa augcagacug     1440

aagauggaca agcugaggcu gaaaggagug agcuauucau uguguacugc cgcauucacg     1500

uuuacaaaga uuccugcgga aacccuccau ggcacuguca ccguggaggu ucaguacgcu     1560

gguacagacg ggccaugcaa agugcccgcc caaauggcag uagauaugca gacccugacu     1620

ccugucggcc gguuaauaac agccaaccca gugaucacgg aaaguaccga gaacuccaag     1680

augaugcugg aacuagaucc cccguucgga gacagcuaca uuguuaucgg cgugggggag     1740

aaaaagauaa cucaccacug gcaucgcucu ggaucaacaa uugguaaggc uuuugaggcc     1800

accgucagag gcgcaaaaag gauggcugug cucggggaca cagccuggga uuucggaagu     1860

guaggcggug cgcugaauag ccuugggaag ggcauccacc agaucuucgg agccgcuuuu     1920

aaauccuugu ucggcgggau gucuugguuu ucgcaaauuc ugaucggaac ucuccugaug     1980

uggcuuggcc ugaacaccaa gaaugguagc auuuccuuga ugugucucgc acuuggggga     2040

guguuaauau uccugucaac ggccguuagu gcugacgucg gcugcucugu ggauuuuagc     2100

aaa                                                                   2103


<210>  1678
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1678
agaggccuga gcuccaggaa gcggcgcucu cacgacgugc ucaccgucca guuccuuauc       60

cuggggaugu uguuaaugac aggagguguu acucgacgug gcucagccua cuauauguac      120

cuggauagaa acgacgcugg ggaggcaauu aguuuuccca cgacccuagg aaugaauaaa      180

ugcuauauac aaaucaugga ucucggccau augugugacg ccacaaugag cuacgaaugc      240

ccuaugcugg augagggugu ggaaccagac gauguagacu guuggugcaa cacuaccucc      300

acaugggugg ucuaugggac cugucaccau aagaaaggag aggcgaggcg gucucgcaga      360

gcugugacuc uuccgucgca cucaacaagg aagcugcaga cccggagcca gacgugguug      420

gaaagucgcg aguacacuaa acaucucauu cgaguugaga auuggaucuu cagaaacccc      480

ggcuuugccc uggcagccgc ugcaauugcc uggcugcucg gcuccucuac cagccagaag      540

gugaucuauu uagucaugau acugcuuauc gcuccugccu acuccauuag gugcaucggg      600

gugucaaauc gggauuucgu agaaggaaug agugguggca cauggguuga cauuguguug      660

gagcacgggg gaugugucac ugugauggcg caagauaagc caaccgugga caucgaacug      720

gucacaacca cgguuagcaa cauggcagag gugcguucuu acugcuauga ggccuccaua      780

ucagauaugg cuagcgacag ucgcuguccc acucagggcg aagccuaccu agacaaacag      840

ucugauacac aguauguaug caagagaacc cucguggaca gggguugggg gaauggaugu      900

ggccuguuug gcaaaggguc ccuugucacu ugcgcaaagu ucgcuugcag caaaaagaug      960

acaggaaagu cgauucaacc ugagaaccug gaauaccgga ucauguuguc ugugcacggu     1020

ucccagcauu caggcaugau uguuaaugau accgggcacg agacggacga aaaccgagcc     1080

aaaguggaga ucaccccaaa uaguccccgc gcggaggcca cacucggagg cuuugguagc     1140

cugggguuag auugugaacc uagaacuggc cuggacuucu ccgaucucua uuaccuuacc     1200

augaacaaua agcauuggcu gguccacaaa gagugguuuc acgacauccc auugccgugg     1260

caugcaggag cugauacagg cacuccccac uggaacaaua aggaagcccu aguggaguuc     1320

aaagacgcuc augccaagag gcagaccgua guugugcugg ggucucagga aggagcaguc     1380

cacacagccc ucgcuggugc gcuggaggca gagauggaug gcgccaaagg gcggcuuagc     1440

ucaggacauc ugaagugccg cuugaagaug gacaaacuca gacugaaggg cgugaguuau     1500

uccuuaugua ccgcugccuu uacuuucacg aaaauuccug cagaaacacu gcacgguacc     1560

gucacugugg agguucaaua cgccgggacc gacggcccau gcaaggugcc cgcucagaug     1620

gccguagaua ugcagacacu aaccccuguc ggaaggcuca uaacugcaaa cccagugauc     1680

acagaaagca cggagaacuc uaaaaugaug cuggaacuug aucccccguu uggcgacuca     1740

uacauuguua ucgggguggg agagaagaag auaacccacc auuggcaccg uagugguagc     1800

acuauuggca aagcguucga ggcuacaguc cgaggggcca agcggauggc aguguuggga     1860

gacaccgccu gggauuucgg cuccguaggu ggggcucuca auucucuggg caaaggaauc     1920

caucaaaucu uuggcgccgc auucaagucg cuguuugggg gaaugagcug guucucccag     1980

auucuuaucg gcacacuguu gauguggcuc ggucugaaca cuaaaaaugg gucaauuagu     2040

uuaauguguc uggcucuagg aggcgugcuc auauuucugu cuaccgccgu uagcgcggac     2100

gucgguugcu ccguggauuu cucaaag                                         2127


<210>  1679
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1679
agaggccuga gcuccaggaa gcggcgcucu cacgacgugc ucaccgucca guuccuuauc       60

cuggggaugu uguuaaugac aggagccguu acucgacgug guucagcuua cuauauguac      120

cuggauagaa acgacgcagg cgaggccauu aguuuuccca cgacccuagg gaugaauaaa      180

ugcuauauac aaaucaugga ucucggacau augugugacg cgacaaugag cuacgaaugc      240

ccuaugcugg augagggcgu ggaaccagac gauguagacu guuggugcaa cacuaccucc      300

acaugggugg ucuaugguac cugucaccau aagaaagggg aggcuaggcg gucucgcaga      360

gccgugacuc uuccgucgca cucaacaagg aagcugcaga cccggagcca gacgugguug      420

gaaagucgcg aguacacuaa acaucucauu cgaguugaga auuggaucuu cagaaacccc      480

ggauuugcac uggccgcugc agccauugcu uggcugcucg gcuccucuac cagccagaag      540

gugaucuauu uagucaugau acugcuuauc gccccugcgu acuccauuag gugcaucggc      600

gugucaaauc gggauuucgu agaagggaug aguggaggua cauggguuga cauuguguug      660

gagcacggcg ggugugucac ugugauggca caagauaagc caaccgugga caucgaacug      720

gucacaacca cgguuagcaa cauggccgag gugcguucuu acugcuauga ggcuuccaua      780

ucagauaugg ccagcgacag ucgcuguccc acucagggag aagcauaccu agacaaacag      840

ucugauacac aguauguaug caagagaacc cucguggaca ggggcugggg uaaugggugu      900

ggacuguuug gcaaaggcuc ccuugucacu ugcgcuaagu ucgccugcag caaaaagaug      960

acagggaagu cgauucaacc ugagaaccug gaauaccgga ucauguuguc ugugcacgga     1020

ucccagcauu cagguaugau uguuaaugau accggccacg agacggacga aaaccgagcg     1080

aaaguggaga ucaccccaaa uaguccccgc gccgaggcaa cacucggggg auuuggcagc     1140

cuggguuuag auugugaacc uagaacuggg cuggacuucu ccgaucucua uuaccuuacc     1200

augaacaaua agcauuggcu gguccacaaa gagugguuuc acgacauccc auugccgugg     1260

caugcuggcg ccgauacagg aacuccccac uggaacaaua aggaagcucu aguggaguuc     1320

aaagacgccc augcaaagag gcagaccgua guugugcugg gcucucagga aggggccguc     1380

cacacagcuc ucgcgggagc acuggaggcc gagauggaug gugcuaaagg ccggcuuagc     1440

ucagggcauc ugaagugccg cuugaagaug gacaaacuca gacugaaggg agugaguuau     1500

uccuuaugua ccgccgcauu uacuuucacg aaaauuccug ccgaaacacu gcacggcacc     1560

gucacugugg agguucaaua cgcugguacc gacgggccau gcaaggugcc cgcccagaug     1620

gcaguagaua ugcagacacu aaccccuguc ggcaggcuca uaacugcgaa cccagugauc     1680

acagaaagca cggagaacuc uaaaaugaug cuggaacuug aucccccguu uggagacuca     1740

uacauuguua ucggcguggg ggagaagaag auaacccacc auuggcaccg uaguggaagc     1800

acuauuggua aggcuuucga ggccacaguc cggggcgcaa aacgaauggc cguguugggg     1860

gacaccgcuu gggauuucgg auccguaggc ggugcccuca auucucuggg gaaaggcauc     1920

caucaaaucu uuggagcagc uuucaagucg cuguuuggcg ggaugagcug guucucccag     1980

auucuuaucg gaacacuguu gauguggcuc ggccugaaca cuaaaaaugg uucaauuagu     2040

uuaauguguc uggcccuagg gggagugcuc auauuucugu cuaccgcggu uagcgccgac     2100

gucggcugcu ccguggauuu cucaaag                                         2127


<210>  1680
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1680
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

auaguguuag agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac      720

auugagcugg ucaccacgac uguuucaaau auggcugaag ugcguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aaguggaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guggaguuua aagacgcgca cgcaaagagg cagaccguag uugugcuggg cagccaggag     1380

ggggccgucc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagacccug acuccugucg gcagguuaau uacagccaac     1680

ccagugauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggagugcuga uuuuccuguc aacggccguu     2100

agugcugacg ucggcugc                                                   2118


<210>  1681
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1681
aucauuaacg ccagaaagga gaaaaagagg cggggcgcug acaccagcgu ggggauaguc       60

ggacugcucc uuacaacugc aauggccgcg gaaguuacgc gccgagguuc cgcuuacuau      120

auguaccugg aucguaauga cgccggcgag gcaaucucuu uccccaccac auuggggaug      180

aacaaaugcu auauucagau cauggauuua ggacacaugu gugacgccac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaauacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaga aaggggaggc uagaaggagc      360

cggcgcgcag ugacacuacc gucccauucu accagaaagc uccaaacgag gucgcagacu      420

uggcuggagu cacgggaaua caccaagcac cuuauucgcg uugagaacug gaucuuucga      480

aaucccggau ucgcccuggc ugccgcggca auagccuggu ugcucggcag caguacaucc      540

cagaaaguga ucuaucuggu caugauucug cucaucgcuc cugccuacuc uauuagaugc      600

aucggcguga gcaacaggga uuuuguagaa gggauguccg gagguacuug gguugacaua      660

guguuagagc auggcgggug ugucaccgug auggcacagg auaagccaac aguggacauu      720

gagcugguca ccacgacugu uucaaauaug gcugaagugc ggaguuacug cuaugaggcc      780

agcaucucug auauggcguc cgacucacgu ugucccacac aaggagaagc cuaccuugac      840

aaacagagcg auacccagua uguaugcaag cgcacuuugg uggacagagg cugggguaac      900

ggguguggac uguucggcaa aggcagucua gucacaugcg caaaguuugc uugcucuaag      960

aaaaugaccg ggaaguccau ucagccugag aaucucgaau acaggaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugaucguu aacgauacgg gccaugagac cgacgagaau     1080

cgggccaaag uggaaauuac accaaacuca ccccgagcug aggccacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucgc accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga auaacaagca cuggcugguc cauaaagagu gguuccacga cauaccacuc     1260

ccguggcacg caggcgccga uacuggaaca ccccauugga auaacaagga agcucuugug     1320

gaguuuaaag acgcgcacgc aaagagacag accguaguug ugcugggcag ccaggagggg     1380

gccguccaua ccgcuuuggc cggagcacua gaagccgaga uggauggugc uaagggcagg     1440

cuguccucug ggcaccucaa augccggcug aagauggaca aacuucgccu gaagggagug     1500

agcuauucau uguguacugc cgcauucacg uuuacaaaaa ucccugcgga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcu gguacagacg ggccaugcaa ggugcccgcc     1620

caaauggcag uagauaugca gacccugacu ccugucggca gauuaauuac agccaaccca     1680

gugaucacgg aaaguaccga aaauuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uaguuauugg cgugggggag aaaaagauca cucaccacug gcauaggucu     1800

ggaucaacaa ucgguaaggc uuuugaggcc accguccgug gcgcaaaacg gauggcugug     1860

cucggggaca cagccuggga uuucggaagu guaggcggug cgcugaacag ccuugggaaa     1920

ggcauucacc agaucuucgg agccgcuuuu aaguccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugauaggaac ucuccugaug uggcuuggcc ugaauaccaa gaacgguagc     2040

aucuccuuga ugugucucgc auuaggggga gugcugauuu uccugucaac ggccguuagu     2100

gcugacgucg gcugc                                                      2115


<210>  1682
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1682
aucaacgcca gaaaggagaa aaagaggcgg ggcgcugaca ccagcguggg gauugucgga       60

cugcuccuua caacugcaau ggccgcggaa guuacgcgcc gagguuccgc uuacuauaug      120

uaccuggauc guaaugacgc cggcgaggca auaucuuucc ccaccacauu ggggaugaac      180

aaaugcuaua uccagauuau ggauuuagga cacaugugug acgccacuau gucauacgaa      240

ugcccuaugc uggaugaggg cguggaacca gacgauguag acuguuggug caauaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaagaaag gggaggcuag aaggagccgg      360

cgcgcaguga cacuaccguc ccauucuacc agaaagcucc aaacgagguc gcagacuugg      420

cuggagucac gggaauacac caagcaccuu auccgcguug agaacuggau uuuucgaaau      480

cccggauucg cccuggcugc cgcggcaauc gccugguugc ucggcagcag uacaucccag      540

aaagugauau aucuggucau gauccugcuc auugcuccug ccuacucuau cagaugcauu      600

ggcgugagca acagggauuu uguagaaggg auguccggag guacuugggu ugacaucgug      660

uuagagcaug gcgggugugu caccgugaug gcacaggaua agccaacagu ggacauagag      720

cuggucacca cgacuguuuc aaauauggcu gaagugcgga guuacugcua ugaggccagc      780

auuucugaua uggcguccga cucacguugu cccacacaag gagaagccua ccuugacaaa      840

cagagcgaua cccaguaugu augcaagcgc acuuuggugg acagaggcug ggguaacggg      900

uguggacugu ucggcaaagg cagucuaguc acaugcgcaa aguuugcuug cucuaagaaa      960

augaccggga aguccaucca gccugagaau cucgaauaca ggauuaugcu gagcgugcac     1020

ggaucgcaac acucugguau gaucguuaac gauacgggcc augagaccga cgagaaucgg     1080

gccaaagugg aaaucacacc aaacucaccc cgagcugagg ccacucuugg gggauucggc     1140

ucccuggguu uggauuguga accucgcacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaua acaagcacug gcugguccau aaagaguggu uccacgacau uccacucccg     1260

uggcacgcag gcgccgauac uggaacaccc cauuggaaua acaaggaagc ucuuguggag     1320

uuuaaagacg cgcacgcaaa gagacagacc guaguugugc ugggcagcca ggagggggcc     1380

guccauaccg cuuuggccgg agcacuagaa gccgagaugg auggugcuaa gggcaggcug     1440

uccucugggc accucaaaug ccggcugaag auggacaaac uucgccugaa gggagugagc     1500

uauucauugu guacugccgc auucacguuu acaaaaauac cugcggaaac ccuccauggc     1560

acugucaccg uggagguuca guacgcuggu acagacgggc caugcaaggu gcccgcccaa     1620

auggcaguag auaugcagac ccugacuccu gucggcagau uaaucacagc caacccagug     1680

auuacggaaa guaccgaaaa uuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacaucg uuauaggcgu gggggagaaa aagauuacuc accacuggca uaggucugga     1800

ucaacaaucg guaaggcuuu ugaggccacc guccguggcg caaaacggau ggcugugcuc     1860

ggggacacag ccugggauuu cggaagugua ggcggugcgc ugaacagccu ugggaaaggc     1920

auccaccaga uuuucggagc cgcuuuuaag uccuuguucg gcgggauguc uugguuuucg     1980

caaauccuga uuggaacucu ccugaugugg cuuggccuga auaccaagaa cgguagcaua     2040

uccuugaugu gucucgcauu agggggagug cugaucuucc ugucaacggc cguuagugcu     2100

gacgucggcu gc                                                         2112


<210>  1683
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1683
aacgccagaa aggagaaaaa gaggcggggc gcugacacca gcguggggau cgucggacug       60

cuccuuacaa cugcaauggc cgcggaaguu acgcgccgag guuccgcuua cuauauguac      120

cuggaucgua augacgccgg cgaggcaauu ucuuucccca ccacauuggg gaugaacaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg ccacuauguc auacgaaugc      240

ccuaugcugg augagggcgu ggaaccagac gauguagacu guuggugcaa uaccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aggcuagaag gagccggcgc      360

gcagugacac uaccguccca uucuaccaga aagcuccaaa cgaggucgca gacuuggcug      420

gagucacggg aauacaccaa gcaccuuauu cgcguugaga acuggaucuu ucgaaauccc      480

ggauucgccc uggcugccgc ggcaauugcc ugguugcucg gcagcaguac aucccagaaa      540

gugaucuauc uggucaugau acugcucauc gcuccugccu acucuauuag augcaucggc      600

gugagcaaca gggauuuugu agaagggaug uccggaggua cuuggguuga cauuguguua      660

gagcauggcg ggugugucac cgugauggca caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa uauggcugaa gugcggaguu acugcuauga ggccagcaua      780

ucugauaugg cguccgacuc acguuguccc acacaaggag aagccuaccu ugacaaacag      840

agcgauaccc aguauguaug caagcgcacu uugguggaca gaggcugggg uaacgggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgcaaagu uugcuugcuc uaagaaaaug      960

accgggaagu ccauucagcc ugagaaucuc gaauacagga ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaacgau acgggccaug agaccgacga gaaucgggcc     1080

aaaguggaaa ucacaccaaa cucaccccga gcugaggcca cucuuggggg auucggcucc     1140

cuggguuugg auugugaacc ucgcaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaauaaca agcacuggcu gguccauaaa gagugguucc acgacauccc acucccgugg     1260

cacgcaggcg ccgauacugg aacaccccau uggaauaaca aggaagcucu uguggaguuu     1320

aaagacgcgc acgcaaagag acagaccgua guugugcugg gcagccagga gggggccguc     1380

cauaccgcuu uggccggagc acuagaagcc gagauggaug gugcuaaggg caggcugucc     1440

ucugggcacc ucaaaugccg gcugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucauugugua cugccgcauu cacguuuaca aaaauuccug cggaaacccu ccauggcacu     1560

gucaccgugg agguucagua cgcugguaca gacgggccau gcaaggugcc cgcccaaaug     1620

gcaguagaua ugcagacccu gacuccuguc ggcagauuaa uaacagccaa cccagugauc     1680

acggaaagua ccgaaaauuc caagaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaaaaag auaacucacc acuggcauag gucuggauca     1800

acaauuggua aggcuuuuga ggccaccguc cguggcgcaa aacggauggc ugugcucggg     1860

gacacagccu gggauuucgg aaguguaggc ggugcgcuga acagccuugg gaaaggcauc     1920

caccagaucu ucggagccgc uuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaua ccaagaacgg uagcauuucc     2040

uugauguguc ucgcauuagg gggagugcug auauuccugu caacggccgu uagugcugac     2100

gucggcugc                                                             2109


<210>  1684
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1684
gccagaaagg agaaaaagag gcggggcgcu gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug caauggccgc ggaaguuacg cgccgagguu ccgcuuacua uauguaccug      120

gaucguaacg acgccggcga ggcaauuucu uuccccacca cauuggggau gaauaaaugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcca cuaugucaua cgaaugcccu      240

augcuggaug agggcgugga accagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaag aaaggggagg cuagaaggag ccggcgcgca      360

gugacacuac cgucccauuc uaccagaaag cuccaaacga ggucgcagac uuggcuggag      420

ucacgggaau acaccaagca ccuuauucgc guugagaauu ggaucuuucg aaaccccgga      480

uucgcccugg cugccgcggc aauugccugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcu ccugccuacu cuauuagaug caucggcgug      600

agcaauaggg auuuuguaga agggaugucc ggagguacuu ggguugacau uguguuagag      660

cauggcgggu gugucaccgu gauggcacag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcugaagug cggaguuacu gcuaugaggc cagcauaucu      780

gauauggcgu ccgacucacg uugucccaca caaggagaag ccuaccuuga caaacagagc      840

gauacccagu auguaugcaa gcgcacuuug guggacagag gcugggguaa ugggugugga      900

cuguucggca aaggcagucu agucacaugc gcaaaguuug cuugcucuaa gaaaaugacc      960

gggaagucca uucagccuga gaaccucgaa uacaggauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaga ccgacgagaa ccgggccaaa     1080

guggaaauca caccaaauuc accccgagcu gaggccacuc uugggggauu cggcucccug     1140

gguuuggauu gugaaccucg caccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaagc acuggcuggu ccauaaagag ugguuccacg acaucccacu cccguggcac     1260

gcaggcgccg auacuggaac accccauugg aacaauaagg aagcucuugu ggaguuuaaa     1320

gacgcgcacg caaagagaca gaccguaguu gugcugggca gccaggaggg ggccguccau     1380

accgcuuugg ccggagcacu agaagccgag auggauggug cuaagggcag gcuguccucu     1440

gggcaccuca aaugccggcu gaagauggac aaacuucgcc ugaagggagu gagcuauuca     1500

uuguguacug ccgcauucac guuuacaaaa auuccugcgg aaacccucca uggcacuguc     1560

accguggagg uucaguacgc ugguacagac gggccaugca aggugcccgc ccaaauggca     1620

guagauaugc agacccugac uccugucggc agauuaauaa cagccaaccc agugaucacg     1680

gaaaguaccg agaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagguc uggaucaaca     1800

auugguaagg cuuuugaagc caccguccgu ggcgcaaaac ggauggcugu gcucggggac     1860

acagccuggg auuucggaag uguaggcggu gcgcugaaua gccuugggaa aggcauccac     1920

cagaucuucg gagccgcuuu uaaguccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cauuaggggg agugcugaua uuccugucaa cggccguuag ugcugacguc     2100

ggcugc                                                                2106


<210>  1685
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1685
agaaaggaga aaaagaggcg gggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga aguuacgcgc cgagguuccg cguacuauau guaccuggau      120

cguaacgacg cuggcgaggc cauuucuuuc cccaccacau uggggaugaa uaaaugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaagaaa ggggaggcca gaaggagccg gcgcgcugug      360

acacuaccgu cccauucuac cagaaagcuc caaacgaggu cgcagacuug gcuggaguca      420

cgggaauaca ccaagcaccu uauucgcguu gagaauugga ucuuucgaaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uuagaugcau cggcgugagc      600

aauagggauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaagugcgg aguuacugcu augaggcuag cauaucugau      780

auggccuccg acucacguug ucccacacaa ggagaagcgu accuugacaa acagagcgau      840

acccaguaug uaugcaagcg cacuuuggug gacagaggcu gggguaaugg guguggacug      900

uucggcaaag gcagucuagu cacaugcgcc aaguuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac aggaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgagaaccg ggcuaaagug     1080

gaaaucacac caaauucacc ccgagccgag gcuacucuug ggggauucgg cucccugggu     1140

uuggauugug aaccucgcac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaagcacu ggcuggucca uaaagagugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaggaag cccuugugga guuuaaagac     1320

gcucacgcga agagacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga agcagagaug gauggugcca agggcaggcu guccucuggg     1440

caccucaaau gccggcugaa gauggacaaa cuucgccuga agggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaaaauu ccugcagaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcggg uacagacggg ccaugcaagg ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggcaga uuaauaacag caaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc auaggucugg aucaacaauu     1800

gguaaagccu uugaagcuac cguccguggc gccaagcgga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaagg cauccaccag     1920

aucuucggag cggccuuuaa guccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuu uagggggagu gcugauauuc cugucaacgg caguuagugc cgacgucggc     2100

ugc                                                                   2103


<210>  1686
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1686
aaggagaaaa agagaagggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaagu uacgcggcgc gguuccgcgu acuauaugua ccuggaucga      120

aacgacgcug gcgaggccau uucuuucccc accacauugg ggaugaauaa augcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgaaug cccuaugcug      240

gaugagggcg uggaaccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caagaaaggg gaggcccgua gaagcaggcg ggcugugaca      360

cuaccguccc auucuacccg caagcuccaa acgagaucgc agacuuggcu ggagucaagg      420

gaauacacca agcaccuuau ucggguugag aauuggaucu uucgcaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc gaugcaucgg cgugagcaau      600

agagauuuug uagaagggau guccggaggu acuuggguug acauuguguu agagcauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga agugaggagu uacugcuaug aggcuagcau aucugauaug      780

gccuccgacu cacggugucc cacacaagga gaagcguacc uugacaaaca gagcgauacc      840

caguauguau gcaagcguac uuugguggac cgcggcuggg guaaugggug uggacuguuc      900

ggcaaaggca gucuagucac augcgccaag uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugagaaccu cgaauacaga aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gagaccgacg agaacagggc uaaaguggaa     1080

aucacaccaa auucaccccg ggccgaggcu acucuugggg gauucggcuc ccuggguuug     1140

gauugugaac cucgaaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aagcacuggc ugguccauaa agagugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaggaagccc uuguggaguu uaaagacgcu     1320

cacgcgaagc gccagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaagc agagauggau ggugccaagg gcagacuguc cucugggcac     1440

cucaaaugca ggcugaagau ggacaaacuu cggcugaagg gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaaaauuccu gcagaaaccc uccauggcac ugucaccgug     1560

gagguucagu acgcggguac agacgggcca ugcaaggugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggccgcuua auaacagcaa acccagugau cacggaaagu     1680

accgagaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcaua gaucuggauc aacaauuggu     1800

aaagccuuug aagcuaccgu caggggcgcc aagcguaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaaaggcau ccaccagauc     1920

uucggagcgg ccuuuaaguc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcuuuag ggggagugcu gauauuccug ucaacggcag uuagugccga cgucggcugc     2100


<210>  1687
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1687
gagaagaaaa gaaggggcgc cgacaccagc guggggaucg ucggacugcu ccuuacaacu       60

gcuauggcag ccgaaguuac gcggcgcggu uccgcguacu auauguaccu ggaucgaaac      120

gacgcuggcg aggccauuuc uuuccccacc acauugggga ugaauaagug cuauauacag      180

aucauggauu uaggacacau gugugacgca acuaugucau acgaaugccc uaugcuggau      240

gagggcgugg aaccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uaugguacuu gucaucacaa aaagggggag gcccguagaa gcaggcgggc ugugacacua      360

ccgucccauu cuacccgcaa acuccaaacg agaucgcaga cuuggcugga gucaagggaa      420

uacaccaagc accuuauucg gguugagaau uggaucuuuc gcaaccccgg auucgcacug      480

gccgcugccg cgauugcaug guugcucggc agcaguacau cccagaaggu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucgau gcaucggcgu gagcaauaga      600

gauuuuguag aagggauguc cggagguacu uggguugaca uuguguuaga gcauggcggg      660

ugugucaccg ugauggccca ggauaaacca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaagu gaggaguuac ugcuaugagg cuagcauauc ugauauggcc      780

uccgacucac ggugucccac acaaggagaa gcguaccuug acaagcagag cgauacccag      840

uauguaugca aacguacuuu gguggaccgc ggcuggggua augggugugg acuguucggc      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaagaugac cgggaaaucc      960

auucagccug agaaccucga auacagaauc augcugagcg ugcacggauc gcaacacucu     1020

gguaugauug uuaaugauac gggccaugag accgacgaga acagggcuaa gguggaaauc     1080

acaccaaauu caccccgggc cgaggcuacu cuugggggau ucggcucccu ggguuuggau     1140

ugugaaccuc gaaccgggcu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga gugguuccac gacaucccac ucccguggca cgccggcgca     1260

gauacuggaa caccccauug gaacaauaaa gaagcccuug uggaguuuaa ggacgcucac     1320

gcgaaacgcc agaccguagu ugugcugggc agccaggagg gggcagucca uaccgccuug     1380

gcuggagccc uagaagcaga gauggauggu gccaagggca gacuguccuc ugggcaccuc     1440

aagugcaggc ugaaaaugga caagcugcgg cugaaaggag ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gaaacccucc auggcacugu caccguggag     1560

guucaguacg cggguacaga cgggccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg ccgcuuaaua acagcaaacc cagugaucac ggaaaguacc     1680

gagaacucca agaugaugcu ggagcuagau cccccguucg gagacagcua cauuguuauc     1740

ggcguggggg aaaagaaaau aacucaccac uggcauagau cuggaucaac aauugguaag     1800

gccuuugagg cuaccgucag gggcgccaaa cguauggcag ugcucgggga cacagcuugg     1860

gauuucggaa guguaggcgg ugcccugaau agccuuggga agggcaucca ccagaucuuc     1920

ggagcggccu uuaaauccuu guucggcggg augucuuggu uuucgcaaau ucugaucgga     1980

acucuccuga uguggcuugg ccugaacacc aagaauggua gcauuuccuu gaugugucuc     2040

gcucuugggg gaguguuaau auuccuguca acggcaguua gugccgacgu cggcugc        2097


<210>  1688
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1688
aagaaaagaa ggggcgccga caccagcgug gggaucgucg gacugcuccu uacaacugcu       60

auggcagccg agguuacgcg gcgcgguucc gcguacuaua uguaccugga ucgaaacgac      120

gcuggcgaag ccauuucuuu ccccaccaca uuggggauga auaagugcua uauacagauc      180

auggauuuag gacacaugug ugacgcaacu augucauacg agugcccuau gcuggaugaa      240

ggcguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

gguacuuguc aucacaaaaa gggggaagcc cguagaagca ggcgggcugu gacacuaccg      360

ucccauucua cccgcaaacu ccaaacgaga ucgcagacuu ggcuggaguc aagggaguac      420

accaagcacc uuauucgggu ugaaaauugg aucuuucgca accccggauu cgcacuggcc      480

gcugccgcga uugcaugguu gcucggcagc aguacauccc agaaggugau cuaucugguc      540

augauacugc ucaucgcccc ugcuuacucu auucgaugca ucggcgugag caauagagau      600

uuuguagagg ggauguccgg agguacuugg guugacauug uguuagaaca uggcgggugu      660

gucaccguga uggcccagga uaaaccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg cagaggugag gaguuacugc uaugaagcua gcauaucuga uauggccucc      780

gacucacggu gucccacaca aggagaggcg uaccuugaca agcagagcga uacccaguau      840

guaugcaaac guacuuuggu ggaccgcggc ugggguaaug gguguggacu guucggcaag      900

ggcagucuag ucacaugcgc caaauuugca ugcucuaaga agaugaccgg gaaauccauu      960

cagccugaaa accucgagua cagaaucaug cugagcgugc acggaucgca acacucuggu     1020

augauuguua augauacggg ccaugaaacc gacgagaaca gggcuaaggu ggagaucaca     1080

ccaaauucac cccgggccga agcuacucuu gggggauucg gcucccuggg uuuggauugu     1140

gagccucgaa ccgggcucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc cggcgcagau     1260

acuggaacac cccauuggaa caauaaagag gcccuugugg aauuuaagga cgcucacgcg     1320

aaacgccaga ccguaguugu gcugggcagc caggaggggg caguccauac cgccuuggcu     1380

ggagcccuag aggcagaaau ggauggugcc aagggcagac uguccucugg gcaccucaag     1440

ugcaggcuga aaauggacaa gcugcggcug aaaggaguga gcuauucauu guguacugcu     1500

gccuucacgu uuacaaagau uccugcagag acccuccaug gcacugucac cguggaaguu     1560

caguacgcgg guacagacgg gccaugcaaa gugcccgcuc aaauggccgu agauaugcag     1620

acccugacuc cugucggccg cuuaauaaca gcaaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggag acagcuacau uguuaucggc     1740

gugggggaga agaaaauaac ucaccacugg cauagaucug gaucaacaau ugguaaggcc     1800

uuugaagcua ccgucagggg cgccaaacgu auggcagugc ucggggacac agcuugggau     1860

uucggaagug uaggcggugc ccugaauagc cuugggaagg gcauccacca gaucuucgga     1920

gcggccuuua aauccuuguu cggcgggaug ucuugguuuu cgcaaauucu gaucggaacu     1980

cuccugaugu ggcuuggccu gaacaccaag aaugguagca uuuccuugau gugucucgcu     2040

cuugggggag uguuaauauu ccugucaacg gcaguuagug ccgacgucgg cugc           2094


<210>  1689
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1689
aagagaaggg gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug       60

gcagccgagg uuacgcggcg cgguuccgcg uacuauaugu accuggaucg aaacgacgcu      120

ggcgaagcca uuucuuuccc caccacauug gggaugaaua aaugcuauau acagaucaug      180

gauuuaggac acauguguga cgcaacuaug ucauacgagu gcccuaugcu ggaugaaggc      240

guggagccag acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu      300

acuugucauc acaagaaagg ggaagcccgu agaagcaggc gggcugugac acuaccgucc      360

cauucuaccc gcaagcucca aacgagaucg cagacuuggc uggagucaag ggaguacacc      420

aaacaccuua uucggguuga aaauuggauc uuucgcaacc ccggauucgc acuggccgcu      480

gccgcgauug caugguugcu cggcagcagu acaucccaga aggugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cgaugcaucg gcgugagcaa uagagauuuu      600

guagagggga uguccggagg uacuuggguu gacauugugu uagaacaugg cggguguguc      660

accgugaugg cccaggauaa gccaacagug gacaucgagc uggucaccac gacuguuuca      720

aacauggcag aggugaggag uuacugcuau gaagcuagca uaucugauau ggccuccgac      780

ucacgguguc ccacacaagg agaggcguac cuugacaaac agagcgauac ccaguaugua      840

ugcaagcgua cuuuggugga ccgcggcugg gguaaugggu guggacuguu cggcaaaggc      900

agucuaguca caugcgccaa guuugcaugc ucuaaaaaga ugaccgggaa guccauucag      960

ccugaaaacc ucgaguacag aaucaugcug agcgugcacg gaucgcaaca cucugguaug     1020

auuguuaaug auacgggcca ugaaaccgac gagaacaggg cuaaagugga gaucacacca     1080

aauucacccc gggccgaagc uacucuuggg ggauucggcu cccuggguuu ggauugugag     1140

ccucgaaccg ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaagcacugg     1200

cugguccaua aagaaugguu ccacgacauc ccacucccgu ggcacgccgg cgcagauacu     1260

ggaacacccc auuggaacaa uaaggaggcc cuuguggaau uuaaagacgc ucacgcgaag     1320

cgccagaccg uaguugugcu gggcagccag gagggggcag uccauaccgc cuuggcugga     1380

gcccuagagg cagaaaugga uggugccaaa ggcagacugu ccucugggca ccucaagugc     1440

aggcugaaga uggacaaacu ucggcugaag ggagugagcu auucauugug uacugcugcc     1500

uucacguuua caaaaauucc ugcagagacc cuccauggca cugucaccgu ggaaguucag     1560

uacgcgggua cagacgggcc augcaaggug cccgcucaaa uggccguaga uaugcagacc     1620

cugacuccug ucggccgcuu aauaacagca aacccaguga ucacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggagaca gcuacauugu uaucggcgug     1740

ggggagaaga agauaacuca ccacuggcau agaucuggau caacaauugg uaaagccuuu     1800

gaagcuaccg ucaggggcgc caagcguaug gcagugcucg gggacacagc uugggauuuc     1860

ggaaguguag gcggugcccu gaauagccuu gggaaaggca uccaccagau cuucggagcg     1920

gccuuuaagu ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc     1980

cugauguggc uuggccugaa caccaaaaau gguagcauuu ccuugaugug ucucgcuuua     2040

gggggagugc ugauauuccu gucaacggca guuagugccg acgucggcug c              2091


<210>  1690
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1690
agaaggggcg ccgacaccag cguggggauc gucggacugc uccuuacaac ugcuauggca       60

gccgagguua cgcggcgcgg uuccgcguac uauauguacc uggaucgaaa cgacgcuggc      120

gaagccauuu cuuuccccac cacauugggg augaauaagu gcuauauaca gaucauggau      180

uuaggacaca ugugugacgc aacuauguca uacgagugcc cuaugcugga ugaaggcgug      240

gagccagacg auguagacug uuggugcaac accacaagua ccuggguggu cuaugguacu      300

ugucaucaca aaaaggggga agcccguaga agcaggcggg cugugacacu accgucccau      360

ucuacccgca aacuccaaac gagaucgcag acuuggcugg agucaaggga guacaccaag      420

caccuuauuc ggguugaaaa uuggaucuuu cgcaaccccg gauucgcacu ggccgcugcc      480

gcgauugcau gguugcucgg cagcaguaca ucccagaaag ugaucuaucu ggucaugaua      540

cugcucaucg ccccugcuua cucuauucga ugcaucggcg ugagcaauag agauuuugua      600

gaggggaugu ccggagguac uuggguugac auuguguuag aacauggcgg gugugucacc      660

gugauggccc aggauaagcc aacaguggac aucgagcugg ucaccacgac uguuucaaac      720

auggcagagg ugaggaguua cugcuaugaa gcuagcauau cugauauggc cuccgacuca      780

cgguguccca cacaaggaga ggcguaccuu gacaagcaga gcgauaccca guauguaugc      840

aaacguacuu ugguggaccg cggcuggggu aaugggugug gacuguucgg caagggcagu      900

cuagucacau gcgccaaauu ugcaugcucu aagaaaauga ccgggaaguc cauucagccu      960

gaaaaccucg aguacagaau caugcugagc gugcacggau cgcaacacuc ugguaugauu     1020

guuaaugaua cgggccauga aaccgacgag aacagggcua agguggagau cacaccaaau     1080

ucaccccggg ccgaagcuac ucuuggggga uucggcuccc uggguuugga uugugagccu     1140

cgaaccgggc ucgacuuuag ugaucuguau uacuuaacaa ugaacaauaa acacuggcug     1200

guccauaagg aaugguucca cgacauccca cucccguggc acgccggcgc agauacugga     1260

acaccccauu ggaacaauaa agaggcccuu guggaauuua aggacgcuca cgcgaaacgc     1320

cagaccguag uugugcuggg cagccaggag ggggcagucc auaccgccuu ggcuggagcc     1380

cuagaggcag aaauggaugg ugccaagggc agacuguccu cugggcaccu caaaugcagg     1440

cugaagaugg acaagcugcg gcugaaagga gugagcuauu cauuguguac ugcugccuuc     1500

acguuuacaa agauuccugc agagacccuc cauggcacug ucaccgugga aguucaguac     1560

gcggguacag acgggccaug caaagugccc gcucaaaugg ccguagauau gcagacccug     1620

acuccugucg gccgcuuaau aacagcaaac ccagugauca cggagaguac cgaaaacucc     1680

aagaugaugc uggagcuaga ucccccguuc ggagacagcu acauuguuau cggcgugggg     1740

gagaaaaaga uaacucacca cuggcauaga ucuggaucaa caauugguaa ggccuuugaa     1800

gcuaccguca ggggcgccaa acguauggca gugcucgggg acacagcuug ggauuucgga     1860

aguguaggcg gugcccugaa uagccuuggg aagggcaucc accagaucuu cggagcggcc     1920

uuuaaauccu uguucggcgg gaugucuugg uuuucgcaaa uucugaucgg aacucuccug     1980

auguggcuug gccugaacac caagaauggu agcauuuccu ugaugugucu cgcucuuggg     2040

ggaguguuaa uauuccuguc aacggcaguu agugccgacg ucggcugc                  2088


<210>  1691
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1691
agaggcgccg acaccagcgu ggggaucguc ggacugcucc uuacaacugc uauggcagcc       60

gagguuacga ggcgggguuc cgcguacuau auguaccugg aucgcaacga cgcuggcgaa      120

gccauuucuu uccccaccac auuggggaug aauaagugcu auauacagau cauggauuua      180

ggacacaugu gugacgcaac uaugucauac gagugcccua ugcuggauga aggcguggag      240

ccagacgaug uagacuguug gugcaacacc acaaguaccu ggguggucua ugguacuugu      300

caucacaaaa agggggaagc ccgacguagc agaagggcug ugacacuacc gucccauucu      360

acccggaaac uccaaacgcg cucgcagacu uggcuggagu caagagagua caccaagcac      420

cuuauuaggg uugaaaauug gaucuuucgg aaccccggau ucgcacuggc cgcugccgcg      480

auugcauggu ugcucggcag caguacaucc cagaaaguga ucuaucuggu caugauacug      540

cucaucgccc cugcuuacuc uauucgcugc aucggcguga gcaaucgaga uuuuguagag      600

gggauguccg gagguacuug gguugacauu guguuagaac auggcgggug ugucaccgug      660

auggcccagg auaagccaac aguggacauc gagcugguca ccacgacugu uucaaacaug      720

gcagagguga gaaguuacug cuaugaagcu agcauaucug auauggccuc cgacucaagg      780

ugucccacac aaggagaggc guaccuugac aagcagagcg auacccagua uguaugcaaa      840

cggacuuugg uggaccgugg cugggguaau ggguguggac uguucggcaa gggcagucua      900

gucacaugcg ccaaauuugc augcucuaag aaaaugaccg ggaaguccau ucagccugaa      960

aaccucgagu accgcaucau gcugagcgug cacggaucgc aacacucugg uaugauuguu     1020

aaugauacgg gccaugaaac cgacgagaac agagcuaagg uggagaucac accaaauucc     1080

cccagggccg aagcuacucu ugggggauuc ggcucacugg guuuggauug ugagccucgg     1140

accgggcucg acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc     1200

cauaaggaau gguuccacga caucccacuc ccguggcacg ccggcgcaga uacuggaaca     1260

ccccauugga acaauaaaga ggcccuugug gaauuuaagg acgcucacgc gaaacgacag     1320

accguaguug ugcugggcag ccaggagggg gcaguccaua ccgccuuggc uggagcccua     1380

gaggcagaaa uggauggugc caagggccgc cuguccucug ggcaccucaa augcagacug     1440

aagauggaca agcugaggcu gaaaggagug agcuauucau uguguacugc ugccuucacg     1500

uuuacaaaga uuccugcaga gacccuccau ggcacuguca ccguggaagu ucaguacgcg     1560

gguacagacg ggccaugcaa agugcccgcu caaauggccg uagauaugca gacccugacu     1620

ccugucggcc gguuaauaac agcaaaccca gugaucacgg agaguaccga aaacuccaag     1680

augaugcugg agcuagaucc cccguucgga gacagcuaca uuguuaucgg cgugggggag     1740

aaaaagauaa cucaccacug gcaucgcucu ggaucaacaa uugguaaggc cuuugaagcu     1800

accgucagag gcgccaaaag gauggcagug cucggggaca cagcuuggga uuucggaagu     1860

guaggcggug cccugaauag ccuugggaag ggcauccacc agaucuucgg agcggccuuu     1920

aaauccuugu ucggcgggau gucuugguuu ucgcaaauuc ugaucggaac ucuccugaug     1980

uggcuuggcc ugaacaccaa gaaugguagc auuuccuuga ugugucucgc ucuuggggga     2040

guguuaauau uccugucaac ggcaguuagu gccgacgucg gcugc                     2085


<210>  1692
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1692
ggcgccgaca ccagcguggg gaucgucgga cugcuccuua caacugcuau ggcagccgag       60

guuacgagaa gggguuccgc guacuauaug uaccuggauc ggaacgacgc uggcgaagcc      120

auuucuuucc ccaccacauu ggggaugaau aagugcuaua uacagaucau ggauuuagga      180

cacaugugug acgcaacuau gucauacgag ugcccuaugc uggaugaagg cguggagcca      240

gacgauguag acuguuggug caacaccaca aguaccuggg uggucuaugg uacuugucau      300

cacaaaaagg gggaagcccg ccgaagccgu agagcuguga cacuaccguc ccauucuacc      360

aggaaacucc aaacgcgguc gcagacuugg cuggagucac gcgaguacac caagcaccuu      420

auuagaguug aaaauuggau cuuuaggaac cccggauucg cacuggccgc ugccgcgauu      480

gcaugguugc ucggcagcag uacaucccag aaagugaucu aucuggucau gauacugcuc      540

aucgccccug cuuacucuau ucggugcauc ggcgugagca aucgcgauuu uguagagggg      600

auguccggag guacuugggu ugacauugug uuagaacaug gcgggugugu caccgugaug      660

gcccaggaua agccaacagu ggacaucgag cuggucacca cgacuguuuc aaacauggca      720

gaggugcgaa guuacugcua ugaagcuagc auaucugaua uggccuccga cucaagaugu      780

cccacacaag gagaggcgua ccuugacaag cagagcgaua cccaguaugu augcaaaagg      840

acuuuggugg accggggcug ggguaauggg uguggacugu ucggcaaggg cagucuaguc      900

acaugcgcca aauuugcaug cucuaagaaa augaccggga aguccauuca gccugaaaac      960

cucgaguacc guaucaugcu gagcgugcac ggaucgcaac acucugguau gauuguuaau     1020

gauacgggcc augaaaccga cgagaaccgc gcuaaggugg agaucacacc aaauuccccc     1080

agagccgaag cuacucuugg gggauucggc ucacuggguu uggauuguga gccuaggacc     1140

gggcucgacu uuagugaucu guauuacuua acaaugaaca auaaacacug gcugguccau     1200

aaggaauggu uccacgacau cccacucccg uggcacgccg gcgcagauac uggaacaccc     1260

cauuggaaca auaaagaggc ccuuguggaa uuuaaggacg cucacgcgaa acggcagacc     1320

guaguugugc ugggcagcca ggagggggca guccauaccg ccuuggcugg agcccuagag     1380

gcagaaaugg auggugccaa gggccgacug uccucugggc accucaaaug ccgccugaag     1440

auggacaagc ugagacugaa aggagugagc uauucauugu guacugcugc cuucacguuu     1500

acaaagauuc cugcagagac ccuccauggc acugucaccg uggaaguuca guacgcgggu     1560

acagacgggc caugcaaagu gcccgcucaa auggccguag auaugcagac ccugacuccu     1620

gucggcaggu uaauaacagc aaacccagug aucacggaga guaccgaaaa cuccaagaug     1680

augcuggagc uagauccccc guucggagac agcuacauug uuaucggcgu gggggagaaa     1740

aagauaacuc accacuggca ucggucugga ucaacaauug guaaggccuu ugaagcuacc     1800

guccgcggcg ccaaaagaau ggcagugcuc ggggacacag cuugggauuu cggaagugua     1860

ggcggugccc ugaauagccu ugggaagggc auccaccaga ucuucggagc ggccuuuaaa     1920

uccuuguucg gcgggauguc uugguuuucg caaauucuga ucggaacucu ccugaugugg     1980

cuuggccuga acaccaagaa ugguagcauu uccuugaugu gucucgcucu ugggggagug     2040

uuaauauucc ugucaacggc aguuagugcc gacgucggcu gc                        2082


<210>  1693
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1693
gccgacacca gcgugggcau cgucgggcug cuccuuacaa cugcuauggc agccgagguu       60

acgagaaggg gauccgcgua cuauauguac cuggaucgga acgacgcugg ugaagccauu      120

ucuuucccca ccacauuggg caugaauaag ugcuauauac agaucaugga uuuagggcac      180

augugugacg caacuauguc auacgagugc ccuaugcugg augaaggagu ggagccagac      240

gauguagacu guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac      300

aaaaagggug aagcccgccg aagccguaga gcugugacac uaccguccca uucuaccagg      360

aaacuccaaa cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu      420

agaguugaaa auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca      480

ugguugcucg gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc      540

gccccugcuu acucuauucg gugcaucggc gugagcaauc gcgauuuugu agagggcaug      600

uccgggggaa cuuggguuga cauuguguua gaacauggug gcugugucac cgugauggcc      660

caggauaagc caacagugga caucgagcug gucaccacga cuguuucaaa cauggcagag      720

gugcgaaguu acugcuauga agcuagcaua ucugauaugg ccuccgacuc aagauguccc      780

acacaagggg aggcguaccu ugacaagcag agcgauaccc aguauguaug caaaaggacu      840

uugguggacc ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca      900

ugcgccaaau uugcaugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc      960

gaguaccgua ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugau     1020

acgggucaug aaaccgacga gaaccgcgcu aagguggaga ucacaccaaa uucccccaga     1080

gccgaagcua cucuuggcgg guucggauca cugggcuugg auugugagcc uaggaccggu     1140

cucgacuuua gugaucugua uuacuuaaca augaacaaua aacacuggcu gguccauaag     1200

gaaugguucc acgacauccc acucccgugg cacgccgggg cagauacugg cacaccccau     1260

uggaacaaua aagaggcccu uguggaauuu aaggacgcuc acgcgaaacg gcagaccgua     1320

guugugcugg gaagccagga gggcgcaguc cauaccgccu uggcuggggc ccuagaggca     1380

gaaauggaug gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug     1440

gacaaacuua gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca     1500

aagauuccug cagagacccu ccauggaacu gucaccgugg aaguucagua cgcgggcaca     1560

gacgguccau gcaaagugcc cgcucaaaug gccguagaua ugcagacccu gacuccuguc     1620

gggagguuaa uaacagcaaa cccagugauc acggagagua ccgaaaacuc caagaugaug     1680

cuggagcuag aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag     1740

auaacucacc acuggcaucg gucuggguca acaauuggaa aggccuuuga agcuaccguc     1800

cgcggugcca aaagaauggc agugcucggc gacacagcuu gggauuucgg gaguguagga     1860

ggcgcccuga auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc     1920

uuguucggag gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu     1980

ggacugaaca ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua     2040

auauuccugu caacggcagu uagugccgac gucggaugc                            2079


<210>  1694
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1694
gacaccagcg ugggcaucgu cgggcugcuc cuuacaacug ccauggcugc agagguuacg       60

agaaggggau ccgccuacua uauguaccug gaucggaacg acgcggguga agcuauuucu      120

uuccccacca cauugggcau gaauaagugc uauauacaga ucauggauuu agggcacaug      180

ugugacgcca cuaugucaua cgagugcccu augcuggaug aaggagugga gccagacgau      240

guagacuguu ggugcaacac cacaaguacc uggguggucu auggcacuug ucaucacaaa      300

aagggugaag cacgccgaag ccguagagcc gugacacuac cgucccauuc uaccaggaaa      360

cuccaaacgc ggucgcagac uuggcuggag ucacgcgagu acaccaagca ccuuauuaga      420

guugaaaauu ggaucuuuag gaaccccggg uucgcucugg cagccgcugc cauugcgugg      480

uugcucggaa gcaguacauc ccagaaagug aucuaucugg ucaugauacu gcucaucgca      540

cccgccuacu cuauucggug caucggcgug agcaaucgcg auuuuguaga gggcaugucc      600

gggggaacuu ggguugacau uguguuagaa caugguggcu gugucaccgu gauggcucag      660

gauaagccaa caguggacau cgagcugguc accacgacug uuucaaacau ggccgaggug      720

cgaaguuacu gcuaugaagc aagcauaucu gauauggcuu ccgacucaag augucccaca      780

caaggggagg ccuaccuuga caagcagagc gauacccagu auguaugcaa aaggacuuug      840

guggaccggg gauggggcaa ugguuguggg cuguucggaa agggcagucu agucacaugc      900

gcgaaauuug ccugcucuaa gaaaaugacc ggcaagucca uucagccuga aaaccucgag      960

uaccguauca ugcugagcgu gcacgggucg caacacucug gaaugauugu uaaugauacg     1020

ggucaugaaa ccgacgagaa ccgcgcaaag guggagauca caccaaauuc ccccagagcu     1080

gaagccacuc uuggcggguu cggaucacug ggcuuggauu gugagccuag gaccggucuc     1140

gacuuuagug aucuguauua cuuaacaaug aacaauaaac acuggcuggu ccauaaggaa     1200

ugguuccacg acaucccacu cccguggcac gcuggggccg auacuggcac cccucauugg     1260

aacaauaaag aggcacuugu ggaauuuaag gacgcccacg cuaaacggca gacaguaguu     1320

gugcugggaa gccaggaggg cgcgguccau accgcauugg ccggggcucu agaggccgaa     1380

auggauggag caaagggucg acuguccucu ggccaccuca aaugccgccu gaagauggac     1440

aaacuuagac ugaagggggu gagcuauuca uuguguacug ccgcuuucac guuuacaaag     1500

auuccugccg agacccucca uggaacuguc accguggaag uucaguacgc aggcacagac     1560

gguccaugca aagugcccgc gcaaauggcu guagauaugc agacccugac uccugucggg     1620

agguuaauaa cagccaaccc agugaucacg gagaguaccg aaaacuccaa gaugaugcug     1680

gagcuagauc ccccguucgg cgacagcuac auuguuaucg gagugggcga gaaaaagaua     1740

acucaccacu ggcaucgguc ugggucaaca auuggaaagg cauuugaagc caccguccgc     1800

ggugcuaaaa gaauggccgu gcucggcgac acagcauggg auuucgggag uguaggaggc     1860

gcucugaaua gccuugguaa ggggauccac cagaucuucg gcgccgcguu uaaauccuug     1920

uucggaggca ugucuugguu uucgcaaauu cugaucggga cucuccugau guggcuugga     1980

cugaacacca agaauggcag cauuuccuug augugucucg cccugggugg gguguuaaua     2040

uuccugucaa cggcuguuag ugcagacguc ggaugc                               2076


<210>  1695
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1695
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc agaugucgga ugc                                  2073


<210>  1696
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1696
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcagc cguuacgaga       60

aggggauccg cguacuauau guaccuggac cggaacgaug cuggugaggc cauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugcaacua ugucauacga augcccuaug cuggacgagg gaguggaacc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaggccc gccgaagccg uagagcugug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaauca cgcgaguaca ccaagcaccu uauuagaguu      420

gagaauugga ucuuuaggaa ccccggguuc gcacuggccg cugccgcgau ugcaugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcuuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaagg cauguccggg      600

ggaacuuggg uugauauugu guuagagcau gguggcugug ucaccgugau ggcccaggac      660

aagccaacag uggauaucga acuggucacc acgacuguuu caaacauggc agaggugcga      720

aguuacugcu augaggcuag cauaucugac auggccuccg auucaagaug ucccacacaa      780

ggggaagcgu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcc      900

aaauuugcau gcucuaagaa aaugaccggc aaguccauuc agccugagaa ccucgaauac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugagaccg augaaaaccg cgcuaaggug gagaucacac caaauucccc cagagccgag     1080

gcuacucuug gcggguucgg aucacugggc uuggacugug aaccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggagugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcagaca cuggcacacc ccauuggaac     1260

aauaaagaag cccuugugga guuuaaggau gcucacgcga aacggcagac cguaguugug     1320

cugggaagcc aggaaggcgc aguccauacc gccuuggcug gggcccuaga ggcagagaug     1380

gacggagcca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugcug ccuucacguu uacaaagauu     1500

ccugcagaaa cccuccaugg aacugucacc guggagguuc aguacgcggg cacagacggu     1560

ccaugcaaag ugcccgcuca aauggccgua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag caaacccagu gaucacggaa aguaccgaga acuccaagau gaugcuggaa     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggccu uugaggcuac cguccgcggu     1800

gccaaaagaa uggcagugcu cggcgauaca gcuugggacu ucgggagugu aggaggcgcc     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg cggccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgcuc uggguggggu guuaauauuc     2040

cugucaacgg caguuagugc cgaugucgga ugc                                  2073


<210>  1697
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1697
agaggcgccg acaccagcgu ggggaucguc ggacugcucc uuacaacugc uauggcagcc       60

gcgguuacga ggcgggguuc cgcuuacuau auguaccugg aucgcaacga cgccggcgag      120

gcaauuucuu uccccaccac auuggggaug aauaagugcu auauacagau cauggauuua      180

ggacacaugu gugacgccac uaugucauac gaaugcccua ugcuggauga gggcguggaa      240

ccagacgaug uagacuguug gugcaacacc acaaguaccu ggguggucua ugguacuugu      300

caucacaaaa agggggaggc ucgacguagc agaagggcag ugacacuacc gucccauucu      360

acccggaaac uccaaacgcg cucgcagacu uggcuggaau caagagagua caccaagcac      420

cuuauuaggg uugagaauug gaucuuucgg aaccccggau ucgcccuggc ugccgcggca      480

auugccuggu ugcucggcag caguacaucc cagaaaguga ucuaucuggu caugauacug      540

cucaucgcuc cugccuacuc uauucgcugc aucggcguga gcaaucgaga uuuuguagaa      600

gggauguccg gagguacuug gguugacauu guguuagagc auggcgggug ugucaccgug      660

auggcacagg auaagccaac aguggacauc gaacugguca ccacgacugu uucaaacaug      720

gcugagguga gaaguuacug cuaugaggcc agcauaucug auauggcguc cgacucaagg      780

ugucccacac aaggagaagc cuaccuugac aagcagagcg auacccagua uguaugcaaa      840

cggacuuugg uggaccgugg cugggguaau ggguguggac uguucggcaa gggcagucua      900

gucacaugcg caaaauuugc uugcucuaag aaaaugaccg ggaaguccau ucagccugag      960

aaccucgaau accgcaucau gcugagcgug cacggaucgc aacacucugg uaugauuguu     1020

aaugauacgg gccaugagac cgacgaaaac agagccaagg uggagaucac accaaauucc     1080

cccagggcug aggccacucu ugggggauuc ggcucacugg guuuggauug ugaaccucgg     1140

accgggcucg acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc     1200

cauaaggagu gguuccacga caucccacuc ccguggcacg caggcgccga uacuggaaca     1260

ccccauugga acaauaaaga agcucuugug gaguuuaagg acgcgcacgc aaaacgacag     1320

accguaguug ugcugggcag ccaggaaggg gccguccaua ccgcuuuggc cggagcacua     1380

gaggccgaga uggauggugc uaagggccgc cuguccucug ggcaccucaa augcagacug     1440

aagauggaca agcugaggcu gaaaggagug agcuauucau uguguacugc cgcauucacg     1500

uuuacaaaga uuccugcgga aacccuccau ggcacuguca ccguggaggu ucaguacgcu     1560

gguacagacg ggccaugcaa agugcccgcc caaauggcag uagauaugca gacccugacu     1620

ccugucggcc gguuaauaac agccaaccca gugaucacgg aaaguaccga gaacuccaag     1680

augaugcugg aacuagaucc cccguucgga gacagcuaca uuguuaucgg cgugggggag     1740

aaaaagauaa cucaccacug gcaucgcucu ggaucaacaa uugguaaggc uuuugaggcc     1800

accgucagag gcgcaaaaag gauggcugug cucggggaca cagccuggga uuucggaagu     1860

guaggcggug cgcugaauag ccuugggaag ggcauccacc agaucuucgg agccgcuuuu     1920

aaauccuugu ucggcgggau gucuugguuu ucgcaaauuc ugaucggaac ucuccugaug     1980

uggcuuggcc ugaacaccaa gaaugguagc auuuccuuga ugugucucgc acuuggggga     2040

guguuaauau uccugucaac ggccguuagu gcugacgucg gcugc                     2085


<210>  1698
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1698
agaggccuga gcuccaggaa gcggcgcucu cacgacgugc ucaccgucca guuccuuauc       60

cuggggaugu uguuaaugac aggagguguu acucgacgug gcucagccua cuauauguac      120

cuggauagaa acgacgcugg ggaggcaauu aguuuuccca cgacccuagg aaugaauaaa      180

ugcuauauac aaaucaugga ucucggccau augugugacg ccacaaugag cuacgaaugc      240

ccuaugcugg augagggugu ggaaccagac gauguagacu guuggugcaa cacuaccucc      300

acaugggugg ucuaugggac cugucaccau aagaaaggag aggcgaggcg gucucgcaga      360

gcugugacuc uuccgucgca cucaacaagg aagcugcaga cccggagcca gacgugguug      420

gaaagucgcg aguacacuaa acaucucauu cgaguugaga auuggaucuu cagaaacccc      480

ggcuuugccc uggcagccgc ugcaauugcc uggcugcucg gcuccucuac cagccagaag      540

gugaucuauu uagucaugau acugcuuauc gcuccugccu acuccauuag gugcaucggg      600

gugucaaauc gggauuucgu agaaggaaug agugguggca cauggguuga cauuguguug      660

gagcacgggg gaugugucac ugugauggcg caagauaagc caaccgugga caucgaacug      720

gucacaacca cgguuagcaa cauggcagag gugcguucuu acugcuauga ggccuccaua      780

ucagauaugg cuagcgacag ucgcuguccc acucagggcg aagccuaccu agacaaacag      840

ucugauacac aguauguaug caagagaacc cucguggaca gggguugggg gaauggaugu      900

ggccuguuug gcaaaggguc ccuugucacu ugcgcaaagu ucgcuugcag caaaaagaug      960

acaggaaagu cgauucaacc ugagaaccug gaauaccgga ucauguuguc ugugcacggu     1020

ucccagcauu caggcaugau uguuaaugau accgggcacg agacggacga aaaccgagcc     1080

aaaguggaga ucaccccaaa uaguccccgc gcggaggcca cacucggagg cuuugguagc     1140

cugggguuag auugugaacc uagaacuggc cuggacuucu ccgaucucua uuaccuuacc     1200

augaacaaua agcauuggcu gguccacaaa gagugguuuc acgacauccc auugccgugg     1260

caugcaggag cugauacagg cacuccccac uggaacaaua aggaagcccu aguggaguuc     1320

aaagacgcuc augccaagag gcagaccgua guugugcugg ggucucagga aggagcaguc     1380

cacacagccc ucgcuggugc gcuggaggca gagauggaug gcgccaaagg gcggcuuagc     1440

ucaggacauc ugaagugccg cuugaagaug gacaaacuca gacugaaggg cgugaguuau     1500

uccuuaugua ccgcugccuu uacuuucacg aaaauuccug cagaaacacu gcacgguacc     1560

gucacugugg agguucaaua cgccgggacc gacggcccau gcaaggugcc cgcucagaug     1620

gccguagaua ugcagacacu aaccccuguc ggaaggcuca uaacugcaaa cccagugauc     1680

acagaaagca cggagaacuc uaaaaugaug cuggaacuug aucccccguu uggcgacuca     1740

uacauuguua ucgggguggg agagaagaag auaacccacc auuggcaccg uagugguagc     1800

acuauuggca aagcguucga ggcuacaguc cgaggggcca agcggauggc aguguuggga     1860

gacaccgccu gggauuucgg cuccguaggu ggggcucuca auucucuggg caaaggaauc     1920

caucaaaucu uuggcgccgc auucaagucg cuguuugggg gaaugagcug guucucccag     1980

auucuuaucg gcacacuguu gauguggcuc ggucugaaca cuaaaaaugg gucaauuagu     2040

uuaauguguc uggcucuagg aggcgugcuc auauuucugu cuaccgccgu uagcgcggac     2100

gucgguugc                                                             2109


<210>  1699
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1699
agaggccuga gcuccaggaa gcggcgcucu cacgacgugc ucaccgucca guuccuuauc       60

cuggggaugu uguuaaugac aggagccguu acucgacgug guucagcuua cuauauguac      120

cuggauagaa acgacgcagg cgaggccauu aguuuuccca cgacccuagg gaugaauaaa      180

ugcuauauac aaaucaugga ucucggacau augugugacg cgacaaugag cuacgaaugc      240

ccuaugcugg augagggcgu ggaaccagac gauguagacu guuggugcaa cacuaccucc      300

acaugggugg ucuaugguac cugucaccau aagaaagggg aggcuaggcg gucucgcaga      360

gccgugacuc uuccgucgca cucaacaagg aagcugcaga cccggagcca gacgugguug      420

gaaagucgcg aguacacuaa acaucucauu cgaguugaga auuggaucuu cagaaacccc      480

ggauuugcac uggccgcugc agccauugcu uggcugcucg gcuccucuac cagccagaag      540

gugaucuauu uagucaugau acugcuuauc gccccugcgu acuccauuag gugcaucggc      600

gugucaaauc gggauuucgu agaagggaug aguggaggua cauggguuga cauuguguug      660

gagcacggcg ggugugucac ugugauggca caagauaagc caaccgugga caucgaacug      720

gucacaacca cgguuagcaa cauggccgag gugcguucuu acugcuauga ggcuuccaua      780

ucagauaugg ccagcgacag ucgcuguccc acucagggag aagcauaccu agacaaacag      840

ucugauacac aguauguaug caagagaacc cucguggaca ggggcugggg uaaugggugu      900

ggacuguuug gcaaaggcuc ccuugucacu ugcgcuaagu ucgccugcag caaaaagaug      960

acagggaagu cgauucaacc ugagaaccug gaauaccgga ucauguuguc ugugcacgga     1020

ucccagcauu cagguaugau uguuaaugau accggccacg agacggacga aaaccgagcg     1080

aaaguggaga ucaccccaaa uaguccccgc gccgaggcaa cacucggggg auuuggcagc     1140

cuggguuuag auugugaacc uagaacuggg cuggacuucu ccgaucucua uuaccuuacc     1200

augaacaaua agcauuggcu gguccacaaa gagugguuuc acgacauccc auugccgugg     1260

caugcuggcg ccgauacagg aacuccccac uggaacaaua aggaagcucu aguggaguuc     1320

aaagacgccc augcaaagag gcagaccgua guugugcugg gcucucagga aggggccguc     1380

cacacagcuc ucgcgggagc acuggaggcc gagauggaug gugcuaaagg ccggcuuagc     1440

ucagggcauc ugaagugccg cuugaagaug gacaaacuca gacugaaggg agugaguuau     1500

uccuuaugua ccgccgcauu uacuuucacg aaaauuccug ccgaaacacu gcacggcacc     1560

gucacugugg agguucaaua cgcugguacc gacgggccau gcaaggugcc cgcccagaug     1620

gcaguagaua ugcagacacu aaccccuguc ggcaggcuca uaacugcgaa cccagugauc     1680

acagaaagca cggagaacuc uaaaaugaug cuggaacuug aucccccguu uggagacuca     1740

uacauuguua ucggcguggg ggagaagaag auaacccacc auuggcaccg uaguggaagc     1800

acuauuggua aggcuuucga ggccacaguc cggggcgcaa aacgaauggc cguguugggg     1860

gacaccgcuu gggauuucgg auccguaggc ggugcccuca auucucuggg gaaaggcauc     1920

caucaaaucu uuggagcagc uuucaagucg cuguuuggcg ggaugagcug guucucccag     1980

auucuuaucg gaacacuguu gauguggcuc ggccugaaca cuaaaaaugg uucaauuagu     2040

uuaauguguc uggcccuagg gggagugcuc auauuucugu cuaccgcggu uagcgccgac     2100

gucggcugc                                                             2109


<210>  1700
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1700
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

auaguguuag agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac      720

auugagcugg ucaccacgac uguuucaaau auggcugaag ugcguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aaguggaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guggaguuua aagacgcgca cgcaaagagg cagaccguag uugugcuggg cagccaggag     1380

ggggccgucc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagacccug acuccugucg gcagguuaau uacagccaac     1680

ccagugauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggagugcuga uuuuccuguc aacggccguu     2100

agugcugac                                                             2109


<210>  1701
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1701
aucauuaacg ccagaaagga gaaaaagagg cggggcgcug acaccagcgu ggggauaguc       60

ggacugcucc uuacaacugc aauggccgcg gaaguuacgc gccgagguuc cgcuuacuau      120

auguaccugg aucguaauga cgccggcgag gcaaucucuu uccccaccac auuggggaug      180

aacaaaugcu auauucagau cauggauuua ggacacaugu gugacgccac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaauacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaga aaggggaggc uagaaggagc      360

cggcgcgcag ugacacuacc gucccauucu accagaaagc uccaaacgag gucgcagacu      420

uggcuggagu cacgggaaua caccaagcac cuuauucgcg uugagaacug gaucuuucga      480

aaucccggau ucgcccuggc ugccgcggca auagccuggu ugcucggcag caguacaucc      540

cagaaaguga ucuaucuggu caugauucug cucaucgcuc cugccuacuc uauuagaugc      600

aucggcguga gcaacaggga uuuuguagaa gggauguccg gagguacuug gguugacaua      660

guguuagagc auggcgggug ugucaccgug auggcacagg auaagccaac aguggacauu      720

gagcugguca ccacgacugu uucaaauaug gcugaagugc ggaguuacug cuaugaggcc      780

agcaucucug auauggcguc cgacucacgu ugucccacac aaggagaagc cuaccuugac      840

aaacagagcg auacccagua uguaugcaag cgcacuuugg uggacagagg cugggguaac      900

ggguguggac uguucggcaa aggcagucua gucacaugcg caaaguuugc uugcucuaag      960

aaaaugaccg ggaaguccau ucagccugag aaucucgaau acaggaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugaucguu aacgauacgg gccaugagac cgacgagaau     1080

cgggccaaag uggaaauuac accaaacuca ccccgagcug aggccacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucgc accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga auaacaagca cuggcugguc cauaaagagu gguuccacga cauaccacuc     1260

ccguggcacg caggcgccga uacuggaaca ccccauugga auaacaagga agcucuugug     1320

gaguuuaaag acgcgcacgc aaagagacag accguaguug ugcugggcag ccaggagggg     1380

gccguccaua ccgcuuuggc cggagcacua gaagccgaga uggauggugc uaagggcagg     1440

cuguccucug ggcaccucaa augccggcug aagauggaca aacuucgccu gaagggagug     1500

agcuauucau uguguacugc cgcauucacg uuuacaaaaa ucccugcgga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcu gguacagacg ggccaugcaa ggugcccgcc     1620

caaauggcag uagauaugca gacccugacu ccugucggca gauuaauuac agccaaccca     1680

gugaucacgg aaaguaccga aaauuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uaguuauugg cgugggggag aaaaagauca cucaccacug gcauaggucu     1800

ggaucaacaa ucgguaaggc uuuugaggcc accguccgug gcgcaaaacg gauggcugug     1860

cucggggaca cagccuggga uuucggaagu guaggcggug cgcugaacag ccuugggaaa     1920

ggcauucacc agaucuucgg agccgcuuuu aaguccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugauaggaac ucuccugaug uggcuuggcc ugaauaccaa gaacgguagc     2040

aucuccuuga ugugucucgc auuaggggga gugcugauuu uccugucaac ggccguuagu     2100

gcugac                                                                2106


<210>  1702
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1702
aucaacgcca gaaaggagaa aaagaggcgg ggcgcugaca ccagcguggg gauugucgga       60

cugcuccuua caacugcaau ggccgcggaa guuacgcgcc gagguuccgc uuacuauaug      120

uaccuggauc guaaugacgc cggcgaggca auaucuuucc ccaccacauu ggggaugaac      180

aaaugcuaua uccagauuau ggauuuagga cacaugugug acgccacuau gucauacgaa      240

ugcccuaugc uggaugaggg cguggaacca gacgauguag acuguuggug caauaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaagaaag gggaggcuag aaggagccgg      360

cgcgcaguga cacuaccguc ccauucuacc agaaagcucc aaacgagguc gcagacuugg      420

cuggagucac gggaauacac caagcaccuu auccgcguug agaacuggau uuuucgaaau      480

cccggauucg cccuggcugc cgcggcaauc gccugguugc ucggcagcag uacaucccag      540

aaagugauau aucuggucau gauccugcuc auugcuccug ccuacucuau cagaugcauu      600

ggcgugagca acagggauuu uguagaaggg auguccggag guacuugggu ugacaucgug      660

uuagagcaug gcgggugugu caccgugaug gcacaggaua agccaacagu ggacauagag      720

cuggucacca cgacuguuuc aaauauggcu gaagugcgga guuacugcua ugaggccagc      780

auuucugaua uggcguccga cucacguugu cccacacaag gagaagccua ccuugacaaa      840

cagagcgaua cccaguaugu augcaagcgc acuuuggugg acagaggcug ggguaacggg      900

uguggacugu ucggcaaagg cagucuaguc acaugcgcaa aguuugcuug cucuaagaaa      960

augaccggga aguccaucca gccugagaau cucgaauaca ggauuaugcu gagcgugcac     1020

ggaucgcaac acucugguau gaucguuaac gauacgggcc augagaccga cgagaaucgg     1080

gccaaagugg aaaucacacc aaacucaccc cgagcugagg ccacucuugg gggauucggc     1140

ucccuggguu uggauuguga accucgcacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaua acaagcacug gcugguccau aaagaguggu uccacgacau uccacucccg     1260

uggcacgcag gcgccgauac uggaacaccc cauuggaaua acaaggaagc ucuuguggag     1320

uuuaaagacg cgcacgcaaa gagacagacc guaguugugc ugggcagcca ggagggggcc     1380

guccauaccg cuuuggccgg agcacuagaa gccgagaugg auggugcuaa gggcaggcug     1440

uccucugggc accucaaaug ccggcugaag auggacaaac uucgccugaa gggagugagc     1500

uauucauugu guacugccgc auucacguuu acaaaaauac cugcggaaac ccuccauggc     1560

acugucaccg uggagguuca guacgcuggu acagacgggc caugcaaggu gcccgcccaa     1620

auggcaguag auaugcagac ccugacuccu gucggcagau uaaucacagc caacccagug     1680

auuacggaaa guaccgaaaa uuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacaucg uuauaggcgu gggggagaaa aagauuacuc accacuggca uaggucugga     1800

ucaacaaucg guaaggcuuu ugaggccacc guccguggcg caaaacggau ggcugugcuc     1860

ggggacacag ccugggauuu cggaagugua ggcggugcgc ugaacagccu ugggaaaggc     1920

auccaccaga uuuucggagc cgcuuuuaag uccuuguucg gcgggauguc uugguuuucg     1980

caaauccuga uuggaacucu ccugaugugg cuuggccuga auaccaagaa cgguagcaua     2040

uccuugaugu gucucgcauu agggggagug cugaucuucc ugucaacggc cguuagugcu     2100

gac                                                                   2103


<210>  1703
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1703
aacgccagaa aggagaaaaa gaggcggggc gcugacacca gcguggggau cgucggacug       60

cuccuuacaa cugcaauggc cgcggaaguu acgcgccgag guuccgcuua cuauauguac      120

cuggaucgua augacgccgg cgaggcaauu ucuuucccca ccacauuggg gaugaacaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg ccacuauguc auacgaaugc      240

ccuaugcugg augagggcgu ggaaccagac gauguagacu guuggugcaa uaccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aggcuagaag gagccggcgc      360

gcagugacac uaccguccca uucuaccaga aagcuccaaa cgaggucgca gacuuggcug      420

gagucacggg aauacaccaa gcaccuuauu cgcguugaga acuggaucuu ucgaaauccc      480

ggauucgccc uggcugccgc ggcaauugcc ugguugcucg gcagcaguac aucccagaaa      540

gugaucuauc uggucaugau acugcucauc gcuccugccu acucuauuag augcaucggc      600

gugagcaaca gggauuuugu agaagggaug uccggaggua cuuggguuga cauuguguua      660

gagcauggcg ggugugucac cgugauggca caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa uauggcugaa gugcggaguu acugcuauga ggccagcaua      780

ucugauaugg cguccgacuc acguuguccc acacaaggag aagccuaccu ugacaaacag      840

agcgauaccc aguauguaug caagcgcacu uugguggaca gaggcugggg uaacgggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgcaaagu uugcuugcuc uaagaaaaug      960

accgggaagu ccauucagcc ugagaaucuc gaauacagga ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaacgau acgggccaug agaccgacga gaaucgggcc     1080

aaaguggaaa ucacaccaaa cucaccccga gcugaggcca cucuuggggg auucggcucc     1140

cuggguuugg auugugaacc ucgcaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaauaaca agcacuggcu gguccauaaa gagugguucc acgacauccc acucccgugg     1260

cacgcaggcg ccgauacugg aacaccccau uggaauaaca aggaagcucu uguggaguuu     1320

aaagacgcgc acgcaaagag acagaccgua guugugcugg gcagccagga gggggccguc     1380

cauaccgcuu uggccggagc acuagaagcc gagauggaug gugcuaaggg caggcugucc     1440

ucugggcacc ucaaaugccg gcugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucauugugua cugccgcauu cacguuuaca aaaauuccug cggaaacccu ccauggcacu     1560

gucaccgugg agguucagua cgcugguaca gacgggccau gcaaggugcc cgcccaaaug     1620

gcaguagaua ugcagacccu gacuccuguc ggcagauuaa uaacagccaa cccagugauc     1680

acggaaagua ccgaaaauuc caagaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaaaaag auaacucacc acuggcauag gucuggauca     1800

acaauuggua aggcuuuuga ggccaccguc cguggcgcaa aacggauggc ugugcucggg     1860

gacacagccu gggauuucgg aaguguaggc ggugcgcuga acagccuugg gaaaggcauc     1920

caccagaucu ucggagccgc uuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaua ccaagaacgg uagcauuucc     2040

uugauguguc ucgcauuagg gggagugcug auauuccugu caacggccgu uagugcugac     2100


<210>  1704
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1704
gccagaaagg agaaaaagag gcggggcgcu gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug caauggccgc ggaaguuacg cgccgagguu ccgcuuacua uauguaccug      120

gaucguaacg acgccggcga ggcaauuucu uuccccacca cauuggggau gaauaaaugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcca cuaugucaua cgaaugcccu      240

augcuggaug agggcgugga accagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaag aaaggggagg cuagaaggag ccggcgcgca      360

gugacacuac cgucccauuc uaccagaaag cuccaaacga ggucgcagac uuggcuggag      420

ucacgggaau acaccaagca ccuuauucgc guugagaauu ggaucuuucg aaaccccgga      480

uucgcccugg cugccgcggc aauugccugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcu ccugccuacu cuauuagaug caucggcgug      600

agcaauaggg auuuuguaga agggaugucc ggagguacuu ggguugacau uguguuagag      660

cauggcgggu gugucaccgu gauggcacag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcugaagug cggaguuacu gcuaugaggc cagcauaucu      780

gauauggcgu ccgacucacg uugucccaca caaggagaag ccuaccuuga caaacagagc      840

gauacccagu auguaugcaa gcgcacuuug guggacagag gcugggguaa ugggugugga      900

cuguucggca aaggcagucu agucacaugc gcaaaguuug cuugcucuaa gaaaaugacc      960

gggaagucca uucagccuga gaaccucgaa uacaggauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaga ccgacgagaa ccgggccaaa     1080

guggaaauca caccaaauuc accccgagcu gaggccacuc uugggggauu cggcucccug     1140

gguuuggauu gugaaccucg caccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaagc acuggcuggu ccauaaagag ugguuccacg acaucccacu cccguggcac     1260

gcaggcgccg auacuggaac accccauugg aacaauaagg aagcucuugu ggaguuuaaa     1320

gacgcgcacg caaagagaca gaccguaguu gugcugggca gccaggaggg ggccguccau     1380

accgcuuugg ccggagcacu agaagccgag auggauggug cuaagggcag gcuguccucu     1440

gggcaccuca aaugccggcu gaagauggac aaacuucgcc ugaagggagu gagcuauuca     1500

uuguguacug ccgcauucac guuuacaaaa auuccugcgg aaacccucca uggcacuguc     1560

accguggagg uucaguacgc ugguacagac gggccaugca aggugcccgc ccaaauggca     1620

guagauaugc agacccugac uccugucggc agauuaauaa cagccaaccc agugaucacg     1680

gaaaguaccg agaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagguc uggaucaaca     1800

auugguaagg cuuuugaagc caccguccgu ggcgcaaaac ggauggcugu gcucggggac     1860

acagccuggg auuucggaag uguaggcggu gcgcugaaua gccuugggaa aggcauccac     1920

cagaucuucg gagccgcuuu uaaguccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cauuaggggg agugcugaua uuccugucaa cggccguuag ugcugac        2097


<210>  1705
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1705
agaaaggaga aaaagaggcg gggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga aguuacgcgc cgagguuccg cguacuauau guaccuggau      120

cguaacgacg cuggcgaggc cauuucuuuc cccaccacau uggggaugaa uaaaugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaagaaa ggggaggcca gaaggagccg gcgcgcugug      360

acacuaccgu cccauucuac cagaaagcuc caaacgaggu cgcagacuug gcuggaguca      420

cgggaauaca ccaagcaccu uauucgcguu gagaauugga ucuuucgaaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uuagaugcau cggcgugagc      600

aauagggauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaagugcgg aguuacugcu augaggcuag cauaucugau      780

auggccuccg acucacguug ucccacacaa ggagaagcgu accuugacaa acagagcgau      840

acccaguaug uaugcaagcg cacuuuggug gacagaggcu gggguaaugg guguggacug      900

uucggcaaag gcagucuagu cacaugcgcc aaguuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac aggaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgagaaccg ggcuaaagug     1080

gaaaucacac caaauucacc ccgagccgag gcuacucuug ggggauucgg cucccugggu     1140

uuggauugug aaccucgcac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaagcacu ggcuggucca uaaagagugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaggaag cccuugugga guuuaaagac     1320

gcucacgcga agagacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga agcagagaug gauggugcca agggcaggcu guccucuggg     1440

caccucaaau gccggcugaa gauggacaaa cuucgccuga agggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaaaauu ccugcagaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcggg uacagacggg ccaugcaagg ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggcaga uuaauaacag caaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc auaggucugg aucaacaauu     1800

gguaaagccu uugaagcuac cguccguggc gccaagcgga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaagg cauccaccag     1920

aucuucggag cggccuuuaa guccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuu uagggggagu gcugauauuc cugucaacgg caguuagugc cgac           2094


<210>  1706
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1706
aaggagaaaa agagaagggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaagu uacgcggcgc gguuccgcgu acuauaugua ccuggaucga      120

aacgacgcug gcgaggccau uucuuucccc accacauugg ggaugaauaa augcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgaaug cccuaugcug      240

gaugagggcg uggaaccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caagaaaggg gaggcccgua gaagcaggcg ggcugugaca      360

cuaccguccc auucuacccg caagcuccaa acgagaucgc agacuuggcu ggagucaagg      420

gaauacacca agcaccuuau ucggguugag aauuggaucu uucgcaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc gaugcaucgg cgugagcaau      600

agagauuuug uagaagggau guccggaggu acuuggguug acauuguguu agagcauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga agugaggagu uacugcuaug aggcuagcau aucugauaug      780

gccuccgacu cacggugucc cacacaagga gaagcguacc uugacaaaca gagcgauacc      840

caguauguau gcaagcguac uuugguggac cgcggcuggg guaaugggug uggacuguuc      900

ggcaaaggca gucuagucac augcgccaag uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugagaaccu cgaauacaga aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gagaccgacg agaacagggc uaaaguggaa     1080

aucacaccaa auucaccccg ggccgaggcu acucuugggg gauucggcuc ccuggguuug     1140

gauugugaac cucgaaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aagcacuggc ugguccauaa agagugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaggaagccc uuguggaguu uaaagacgcu     1320

cacgcgaagc gccagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaagc agagauggau ggugccaagg gcagacuguc cucugggcac     1440

cucaaaugca ggcugaagau ggacaaacuu cggcugaagg gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaaaauuccu gcagaaaccc uccauggcac ugucaccgug     1560

gagguucagu acgcggguac agacgggcca ugcaaggugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggccgcuua auaacagcaa acccagugau cacggaaagu     1680

accgagaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcaua gaucuggauc aacaauuggu     1800

aaagccuuug aagcuaccgu caggggcgcc aagcguaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaaaggcau ccaccagauc     1920

uucggagcgg ccuuuaaguc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcuuuag ggggagugcu gauauuccug ucaacggcag uuagugccga c              2091


<210>  1707
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1707
gagaagaaaa gaaggggcgc cgacaccagc guggggaucg ucggacugcu ccuuacaacu       60

gcuauggcag ccgaaguuac gcggcgcggu uccgcguacu auauguaccu ggaucgaaac      120

gacgcuggcg aggccauuuc uuuccccacc acauugggga ugaauaagug cuauauacag      180

aucauggauu uaggacacau gugugacgca acuaugucau acgaaugccc uaugcuggau      240

gagggcgugg aaccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uaugguacuu gucaucacaa aaagggggag gcccguagaa gcaggcgggc ugugacacua      360

ccgucccauu cuacccgcaa acuccaaacg agaucgcaga cuuggcugga gucaagggaa      420

uacaccaagc accuuauucg gguugagaau uggaucuuuc gcaaccccgg auucgcacug      480

gccgcugccg cgauugcaug guugcucggc agcaguacau cccagaaggu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucgau gcaucggcgu gagcaauaga      600

gauuuuguag aagggauguc cggagguacu uggguugaca uuguguuaga gcauggcggg      660

ugugucaccg ugauggccca ggauaaacca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaagu gaggaguuac ugcuaugagg cuagcauauc ugauauggcc      780

uccgacucac ggugucccac acaaggagaa gcguaccuug acaagcagag cgauacccag      840

uauguaugca aacguacuuu gguggaccgc ggcuggggua augggugugg acuguucggc      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaagaugac cgggaaaucc      960

auucagccug agaaccucga auacagaauc augcugagcg ugcacggauc gcaacacucu     1020

gguaugauug uuaaugauac gggccaugag accgacgaga acagggcuaa gguggaaauc     1080

acaccaaauu caccccgggc cgaggcuacu cuugggggau ucggcucccu ggguuuggau     1140

ugugaaccuc gaaccgggcu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga gugguuccac gacaucccac ucccguggca cgccggcgca     1260

gauacuggaa caccccauug gaacaauaaa gaagcccuug uggaguuuaa ggacgcucac     1320

gcgaaacgcc agaccguagu ugugcugggc agccaggagg gggcagucca uaccgccuug     1380

gcuggagccc uagaagcaga gauggauggu gccaagggca gacuguccuc ugggcaccuc     1440

aagugcaggc ugaaaaugga caagcugcgg cugaaaggag ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gaaacccucc auggcacugu caccguggag     1560

guucaguacg cggguacaga cgggccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg ccgcuuaaua acagcaaacc cagugaucac ggaaaguacc     1680

gagaacucca agaugaugcu ggagcuagau cccccguucg gagacagcua cauuguuauc     1740

ggcguggggg aaaagaaaau aacucaccac uggcauagau cuggaucaac aauugguaag     1800

gccuuugagg cuaccgucag gggcgccaaa cguauggcag ugcucgggga cacagcuugg     1860

gauuucggaa guguaggcgg ugcccugaau agccuuggga agggcaucca ccagaucuuc     1920

ggagcggccu uuaaauccuu guucggcggg augucuuggu uuucgcaaau ucugaucgga     1980

acucuccuga uguggcuugg ccugaacacc aagaauggua gcauuuccuu gaugugucuc     2040

gcucuugggg gaguguuaau auuccuguca acggcaguua gugccgac                  2088


<210>  1708
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1708
aagaaaagaa ggggcgccga caccagcgug gggaucgucg gacugcuccu uacaacugcu       60

auggcagccg agguuacgcg gcgcgguucc gcguacuaua uguaccugga ucgaaacgac      120

gcuggcgaag ccauuucuuu ccccaccaca uuggggauga auaagugcua uauacagauc      180

auggauuuag gacacaugug ugacgcaacu augucauacg agugcccuau gcuggaugaa      240

ggcguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

gguacuuguc aucacaaaaa gggggaagcc cguagaagca ggcgggcugu gacacuaccg      360

ucccauucua cccgcaaacu ccaaacgaga ucgcagacuu ggcuggaguc aagggaguac      420

accaagcacc uuauucgggu ugaaaauugg aucuuucgca accccggauu cgcacuggcc      480

gcugccgcga uugcaugguu gcucggcagc aguacauccc agaaggugau cuaucugguc      540

augauacugc ucaucgcccc ugcuuacucu auucgaugca ucggcgugag caauagagau      600

uuuguagagg ggauguccgg agguacuugg guugacauug uguuagaaca uggcgggugu      660

gucaccguga uggcccagga uaaaccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg cagaggugag gaguuacugc uaugaagcua gcauaucuga uauggccucc      780

gacucacggu gucccacaca aggagaggcg uaccuugaca agcagagcga uacccaguau      840

guaugcaaac guacuuuggu ggaccgcggc ugggguaaug gguguggacu guucggcaag      900

ggcagucuag ucacaugcgc caaauuugca ugcucuaaga agaugaccgg gaaauccauu      960

cagccugaaa accucgagua cagaaucaug cugagcgugc acggaucgca acacucuggu     1020

augauuguua augauacggg ccaugaaacc gacgagaaca gggcuaaggu ggagaucaca     1080

ccaaauucac cccgggccga agcuacucuu gggggauucg gcucccuggg uuuggauugu     1140

gagccucgaa ccgggcucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc cggcgcagau     1260

acuggaacac cccauuggaa caauaaagag gcccuugugg aauuuaagga cgcucacgcg     1320

aaacgccaga ccguaguugu gcugggcagc caggaggggg caguccauac cgccuuggcu     1380

ggagcccuag aggcagaaau ggauggugcc aagggcagac uguccucugg gcaccucaag     1440

ugcaggcuga aaauggacaa gcugcggcug aaaggaguga gcuauucauu guguacugcu     1500

gccuucacgu uuacaaagau uccugcagag acccuccaug gcacugucac cguggaaguu     1560

caguacgcgg guacagacgg gccaugcaaa gugcccgcuc aaauggccgu agauaugcag     1620

acccugacuc cugucggccg cuuaauaaca gcaaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggag acagcuacau uguuaucggc     1740

gugggggaga agaaaauaac ucaccacugg cauagaucug gaucaacaau ugguaaggcc     1800

uuugaagcua ccgucagggg cgccaaacgu auggcagugc ucggggacac agcuugggau     1860

uucggaagug uaggcggugc ccugaauagc cuugggaagg gcauccacca gaucuucgga     1920

gcggccuuua aauccuuguu cggcgggaug ucuugguuuu cgcaaauucu gaucggaacu     1980

cuccugaugu ggcuuggccu gaacaccaag aaugguagca uuuccuugau gugucucgcu     2040

cuugggggag uguuaauauu ccugucaacg gcaguuagug ccgac                     2085


<210>  1709
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1709
aagagaaggg gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug       60

gcagccgagg uuacgcggcg cgguuccgcg uacuauaugu accuggaucg aaacgacgcu      120

ggcgaagcca uuucuuuccc caccacauug gggaugaaua aaugcuauau acagaucaug      180

gauuuaggac acauguguga cgcaacuaug ucauacgagu gcccuaugcu ggaugaaggc      240

guggagccag acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu      300

acuugucauc acaagaaagg ggaagcccgu agaagcaggc gggcugugac acuaccgucc      360

cauucuaccc gcaagcucca aacgagaucg cagacuuggc uggagucaag ggaguacacc      420

aaacaccuua uucggguuga aaauuggauc uuucgcaacc ccggauucgc acuggccgcu      480

gccgcgauug caugguugcu cggcagcagu acaucccaga aggugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cgaugcaucg gcgugagcaa uagagauuuu      600

guagagggga uguccggagg uacuuggguu gacauugugu uagaacaugg cggguguguc      660

accgugaugg cccaggauaa gccaacagug gacaucgagc uggucaccac gacuguuuca      720

aacauggcag aggugaggag uuacugcuau gaagcuagca uaucugauau ggccuccgac      780

ucacgguguc ccacacaagg agaggcguac cuugacaaac agagcgauac ccaguaugua      840

ugcaagcgua cuuuggugga ccgcggcugg gguaaugggu guggacuguu cggcaaaggc      900

agucuaguca caugcgccaa guuugcaugc ucuaaaaaga ugaccgggaa guccauucag      960

ccugaaaacc ucgaguacag aaucaugcug agcgugcacg gaucgcaaca cucugguaug     1020

auuguuaaug auacgggcca ugaaaccgac gagaacaggg cuaaagugga gaucacacca     1080

aauucacccc gggccgaagc uacucuuggg ggauucggcu cccuggguuu ggauugugag     1140

ccucgaaccg ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaagcacugg     1200

cugguccaua aagaaugguu ccacgacauc ccacucccgu ggcacgccgg cgcagauacu     1260

ggaacacccc auuggaacaa uaaggaggcc cuuguggaau uuaaagacgc ucacgcgaag     1320

cgccagaccg uaguugugcu gggcagccag gagggggcag uccauaccgc cuuggcugga     1380

gcccuagagg cagaaaugga uggugccaaa ggcagacugu ccucugggca ccucaagugc     1440

aggcugaaga uggacaaacu ucggcugaag ggagugagcu auucauugug uacugcugcc     1500

uucacguuua caaaaauucc ugcagagacc cuccauggca cugucaccgu ggaaguucag     1560

uacgcgggua cagacgggcc augcaaggug cccgcucaaa uggccguaga uaugcagacc     1620

cugacuccug ucggccgcuu aauaacagca aacccaguga ucacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggagaca gcuacauugu uaucggcgug     1740

ggggagaaga agauaacuca ccacuggcau agaucuggau caacaauugg uaaagccuuu     1800

gaagcuaccg ucaggggcgc caagcguaug gcagugcucg gggacacagc uugggauuuc     1860

ggaaguguag gcggugcccu gaauagccuu gggaaaggca uccaccagau cuucggagcg     1920

gccuuuaagu ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc     1980

cugauguggc uuggccugaa caccaaaaau gguagcauuu ccuugaugug ucucgcuuua     2040

gggggagugc ugauauuccu gucaacggca guuagugccg ac                        2082


<210>  1710
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1710
agaaggggcg ccgacaccag cguggggauc gucggacugc uccuuacaac ugcuauggca       60

gccgagguua cgcggcgcgg uuccgcguac uauauguacc uggaucgaaa cgacgcuggc      120

gaagccauuu cuuuccccac cacauugggg augaauaagu gcuauauaca gaucauggau      180

uuaggacaca ugugugacgc aacuauguca uacgagugcc cuaugcugga ugaaggcgug      240

gagccagacg auguagacug uuggugcaac accacaagua ccuggguggu cuaugguacu      300

ugucaucaca aaaaggggga agcccguaga agcaggcggg cugugacacu accgucccau      360

ucuacccgca aacuccaaac gagaucgcag acuuggcugg agucaaggga guacaccaag      420

caccuuauuc ggguugaaaa uuggaucuuu cgcaaccccg gauucgcacu ggccgcugcc      480

gcgauugcau gguugcucgg cagcaguaca ucccagaaag ugaucuaucu ggucaugaua      540

cugcucaucg ccccugcuua cucuauucga ugcaucggcg ugagcaauag agauuuugua      600

gaggggaugu ccggagguac uuggguugac auuguguuag aacauggcgg gugugucacc      660

gugauggccc aggauaagcc aacaguggac aucgagcugg ucaccacgac uguuucaaac      720

auggcagagg ugaggaguua cugcuaugaa gcuagcauau cugauauggc cuccgacuca      780

cgguguccca cacaaggaga ggcguaccuu gacaagcaga gcgauaccca guauguaugc      840

aaacguacuu ugguggaccg cggcuggggu aaugggugug gacuguucgg caagggcagu      900

cuagucacau gcgccaaauu ugcaugcucu aagaaaauga ccgggaaguc cauucagccu      960

gaaaaccucg aguacagaau caugcugagc gugcacggau cgcaacacuc ugguaugauu     1020

guuaaugaua cgggccauga aaccgacgag aacagggcua agguggagau cacaccaaau     1080

ucaccccggg ccgaagcuac ucuuggggga uucggcuccc uggguuugga uugugagccu     1140

cgaaccgggc ucgacuuuag ugaucuguau uacuuaacaa ugaacaauaa acacuggcug     1200

guccauaagg aaugguucca cgacauccca cucccguggc acgccggcgc agauacugga     1260

acaccccauu ggaacaauaa agaggcccuu guggaauuua aggacgcuca cgcgaaacgc     1320

cagaccguag uugugcuggg cagccaggag ggggcagucc auaccgccuu ggcuggagcc     1380

cuagaggcag aaauggaugg ugccaagggc agacuguccu cugggcaccu caaaugcagg     1440

cugaagaugg acaagcugcg gcugaaagga gugagcuauu cauuguguac ugcugccuuc     1500

acguuuacaa agauuccugc agagacccuc cauggcacug ucaccgugga aguucaguac     1560

gcggguacag acgggccaug caaagugccc gcucaaaugg ccguagauau gcagacccug     1620

acuccugucg gccgcuuaau aacagcaaac ccagugauca cggagaguac cgaaaacucc     1680

aagaugaugc uggagcuaga ucccccguuc ggagacagcu acauuguuau cggcgugggg     1740

gagaaaaaga uaacucacca cuggcauaga ucuggaucaa caauugguaa ggccuuugaa     1800

gcuaccguca ggggcgccaa acguauggca gugcucgggg acacagcuug ggauuucgga     1860

aguguaggcg gugcccugaa uagccuuggg aagggcaucc accagaucuu cggagcggcc     1920

uuuaaauccu uguucggcgg gaugucuugg uuuucgcaaa uucugaucgg aacucuccug     1980

auguggcuug gccugaacac caagaauggu agcauuuccu ugaugugucu cgcucuuggg     2040

ggaguguuaa uauuccuguc aacggcaguu agugccgac                            2079


<210>  1711
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1711
agaggcgccg acaccagcgu ggggaucguc ggacugcucc uuacaacugc uauggcagcc       60

gagguuacga ggcgggguuc cgcguacuau auguaccugg aucgcaacga cgcuggcgaa      120

gccauuucuu uccccaccac auuggggaug aauaagugcu auauacagau cauggauuua      180

ggacacaugu gugacgcaac uaugucauac gagugcccua ugcuggauga aggcguggag      240

ccagacgaug uagacuguug gugcaacacc acaaguaccu ggguggucua ugguacuugu      300

caucacaaaa agggggaagc ccgacguagc agaagggcug ugacacuacc gucccauucu      360

acccggaaac uccaaacgcg cucgcagacu uggcuggagu caagagagua caccaagcac      420

cuuauuaggg uugaaaauug gaucuuucgg aaccccggau ucgcacuggc cgcugccgcg      480

auugcauggu ugcucggcag caguacaucc cagaaaguga ucuaucuggu caugauacug      540

cucaucgccc cugcuuacuc uauucgcugc aucggcguga gcaaucgaga uuuuguagag      600

gggauguccg gagguacuug gguugacauu guguuagaac auggcgggug ugucaccgug      660

auggcccagg auaagccaac aguggacauc gagcugguca ccacgacugu uucaaacaug      720

gcagagguga gaaguuacug cuaugaagcu agcauaucug auauggccuc cgacucaagg      780

ugucccacac aaggagaggc guaccuugac aagcagagcg auacccagua uguaugcaaa      840

cggacuuugg uggaccgugg cugggguaau ggguguggac uguucggcaa gggcagucua      900

gucacaugcg ccaaauuugc augcucuaag aaaaugaccg ggaaguccau ucagccugaa      960

aaccucgagu accgcaucau gcugagcgug cacggaucgc aacacucugg uaugauuguu     1020

aaugauacgg gccaugaaac cgacgagaac agagcuaagg uggagaucac accaaauucc     1080

cccagggccg aagcuacucu ugggggauuc ggcucacugg guuuggauug ugagccucgg     1140

accgggcucg acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc     1200

cauaaggaau gguuccacga caucccacuc ccguggcacg ccggcgcaga uacuggaaca     1260

ccccauugga acaauaaaga ggcccuugug gaauuuaagg acgcucacgc gaaacgacag     1320

accguaguug ugcugggcag ccaggagggg gcaguccaua ccgccuuggc uggagcccua     1380

gaggcagaaa uggauggugc caagggccgc cuguccucug ggcaccucaa augcagacug     1440

aagauggaca agcugaggcu gaaaggagug agcuauucau uguguacugc ugccuucacg     1500

uuuacaaaga uuccugcaga gacccuccau ggcacuguca ccguggaagu ucaguacgcg     1560

gguacagacg ggccaugcaa agugcccgcu caaauggccg uagauaugca gacccugacu     1620

ccugucggcc gguuaauaac agcaaaccca gugaucacgg agaguaccga aaacuccaag     1680

augaugcugg agcuagaucc cccguucgga gacagcuaca uuguuaucgg cgugggggag     1740

aaaaagauaa cucaccacug gcaucgcucu ggaucaacaa uugguaaggc cuuugaagcu     1800

accgucagag gcgccaaaag gauggcagug cucggggaca cagcuuggga uuucggaagu     1860

guaggcggug cccugaauag ccuugggaag ggcauccacc agaucuucgg agcggccuuu     1920

aaauccuugu ucggcgggau gucuugguuu ucgcaaauuc ugaucggaac ucuccugaug     1980

uggcuuggcc ugaacaccaa gaaugguagc auuuccuuga ugugucucgc ucuuggggga     2040

guguuaauau uccugucaac ggcaguuagu gccgac                               2076


<210>  1712
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1712
ggcgccgaca ccagcguggg gaucgucgga cugcuccuua caacugcuau ggcagccgag       60

guuacgagaa gggguuccgc guacuauaug uaccuggauc ggaacgacgc uggcgaagcc      120

auuucuuucc ccaccacauu ggggaugaau aagugcuaua uacagaucau ggauuuagga      180

cacaugugug acgcaacuau gucauacgag ugcccuaugc uggaugaagg cguggagcca      240

gacgauguag acuguuggug caacaccaca aguaccuggg uggucuaugg uacuugucau      300

cacaaaaagg gggaagcccg ccgaagccgu agagcuguga cacuaccguc ccauucuacc      360

aggaaacucc aaacgcgguc gcagacuugg cuggagucac gcgaguacac caagcaccuu      420

auuagaguug aaaauuggau cuuuaggaac cccggauucg cacuggccgc ugccgcgauu      480

gcaugguugc ucggcagcag uacaucccag aaagugaucu aucuggucau gauacugcuc      540

aucgccccug cuuacucuau ucggugcauc ggcgugagca aucgcgauuu uguagagggg      600

auguccggag guacuugggu ugacauugug uuagaacaug gcgggugugu caccgugaug      660

gcccaggaua agccaacagu ggacaucgag cuggucacca cgacuguuuc aaacauggca      720

gaggugcgaa guuacugcua ugaagcuagc auaucugaua uggccuccga cucaagaugu      780

cccacacaag gagaggcgua ccuugacaag cagagcgaua cccaguaugu augcaaaagg      840

acuuuggugg accggggcug ggguaauggg uguggacugu ucggcaaggg cagucuaguc      900

acaugcgcca aauuugcaug cucuaagaaa augaccggga aguccauuca gccugaaaac      960

cucgaguacc guaucaugcu gagcgugcac ggaucgcaac acucugguau gauuguuaau     1020

gauacgggcc augaaaccga cgagaaccgc gcuaaggugg agaucacacc aaauuccccc     1080

agagccgaag cuacucuugg gggauucggc ucacuggguu uggauuguga gccuaggacc     1140

gggcucgacu uuagugaucu guauuacuua acaaugaaca auaaacacug gcugguccau     1200

aaggaauggu uccacgacau cccacucccg uggcacgccg gcgcagauac uggaacaccc     1260

cauuggaaca auaaagaggc ccuuguggaa uuuaaggacg cucacgcgaa acggcagacc     1320

guaguugugc ugggcagcca ggagggggca guccauaccg ccuuggcugg agcccuagag     1380

gcagaaaugg auggugccaa gggccgacug uccucugggc accucaaaug ccgccugaag     1440

auggacaagc ugagacugaa aggagugagc uauucauugu guacugcugc cuucacguuu     1500

acaaagauuc cugcagagac ccuccauggc acugucaccg uggaaguuca guacgcgggu     1560

acagacgggc caugcaaagu gcccgcucaa auggccguag auaugcagac ccugacuccu     1620

gucggcaggu uaauaacagc aaacccagug aucacggaga guaccgaaaa cuccaagaug     1680

augcuggagc uagauccccc guucggagac agcuacauug uuaucggcgu gggggagaaa     1740

aagauaacuc accacuggca ucggucugga ucaacaauug guaaggccuu ugaagcuacc     1800

guccgcggcg ccaaaagaau ggcagugcuc ggggacacag cuugggauuu cggaagugua     1860

ggcggugccc ugaauagccu ugggaagggc auccaccaga ucuucggagc ggccuuuaaa     1920

uccuuguucg gcgggauguc uugguuuucg caaauucuga ucggaacucu ccugaugugg     1980

cuuggccuga acaccaagaa ugguagcauu uccuugaugu gucucgcucu ugggggagug     2040

uuaauauucc ugucaacggc aguuagugcc gac                                  2073


<210>  1713
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1713
gccgacacca gcgugggcau cgucgggcug cuccuuacaa cugcuauggc agccgagguu       60

acgagaaggg gauccgcgua cuauauguac cuggaucgga acgacgcugg ugaagccauu      120

ucuuucccca ccacauuggg caugaauaag ugcuauauac agaucaugga uuuagggcac      180

augugugacg caacuauguc auacgagugc ccuaugcugg augaaggagu ggagccagac      240

gauguagacu guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac      300

aaaaagggug aagcccgccg aagccguaga gcugugacac uaccguccca uucuaccagg      360

aaacuccaaa cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu      420

agaguugaaa auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca      480

ugguugcucg gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc      540

gccccugcuu acucuauucg gugcaucggc gugagcaauc gcgauuuugu agagggcaug      600

uccgggggaa cuuggguuga cauuguguua gaacauggug gcugugucac cgugauggcc      660

caggauaagc caacagugga caucgagcug gucaccacga cuguuucaaa cauggcagag      720

gugcgaaguu acugcuauga agcuagcaua ucugauaugg ccuccgacuc aagauguccc      780

acacaagggg aggcguaccu ugacaagcag agcgauaccc aguauguaug caaaaggacu      840

uugguggacc ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca      900

ugcgccaaau uugcaugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc      960

gaguaccgua ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugau     1020

acgggucaug aaaccgacga gaaccgcgcu aagguggaga ucacaccaaa uucccccaga     1080

gccgaagcua cucuuggcgg guucggauca cugggcuugg auugugagcc uaggaccggu     1140

cucgacuuua gugaucugua uuacuuaaca augaacaaua aacacuggcu gguccauaag     1200

gaaugguucc acgacauccc acucccgugg cacgccgggg cagauacugg cacaccccau     1260

uggaacaaua aagaggcccu uguggaauuu aaggacgcuc acgcgaaacg gcagaccgua     1320

guugugcugg gaagccagga gggcgcaguc cauaccgccu uggcuggggc ccuagaggca     1380

gaaauggaug gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug     1440

gacaaacuua gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca     1500

aagauuccug cagagacccu ccauggaacu gucaccgugg aaguucagua cgcgggcaca     1560

gacgguccau gcaaagugcc cgcucaaaug gccguagaua ugcagacccu gacuccuguc     1620

gggagguuaa uaacagcaaa cccagugauc acggagagua ccgaaaacuc caagaugaug     1680

cuggagcuag aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag     1740

auaacucacc acuggcaucg gucuggguca acaauuggaa aggccuuuga agcuaccguc     1800

cgcggugcca aaagaauggc agugcucggc gacacagcuu gggauuucgg gaguguagga     1860

ggcgcccuga auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc     1920

uuguucggag gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu     1980

ggacugaaca ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua     2040

auauuccugu caacggcagu uagugccgac                                      2070


<210>  1714
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1714
gacaccagcg ugggcaucgu cgggcugcuc cuuacaacug ccauggcugc agagguuacg       60

agaaggggau ccgccuacua uauguaccug gaucggaacg acgcggguga agcuauuucu      120

uuccccacca cauugggcau gaauaagugc uauauacaga ucauggauuu agggcacaug      180

ugugacgcca cuaugucaua cgagugcccu augcuggaug aaggagugga gccagacgau      240

guagacuguu ggugcaacac cacaaguacc uggguggucu auggcacuug ucaucacaaa      300

aagggugaag cacgccgaag ccguagagcc gugacacuac cgucccauuc uaccaggaaa      360

cuccaaacgc ggucgcagac uuggcuggag ucacgcgagu acaccaagca ccuuauuaga      420

guugaaaauu ggaucuuuag gaaccccggg uucgcucugg cagccgcugc cauugcgugg      480

uugcucggaa gcaguacauc ccagaaagug aucuaucugg ucaugauacu gcucaucgca      540

cccgccuacu cuauucggug caucggcgug agcaaucgcg auuuuguaga gggcaugucc      600

gggggaacuu ggguugacau uguguuagaa caugguggcu gugucaccgu gauggcucag      660

gauaagccaa caguggacau cgagcugguc accacgacug uuucaaacau ggccgaggug      720

cgaaguuacu gcuaugaagc aagcauaucu gauauggcuu ccgacucaag augucccaca      780

caaggggagg ccuaccuuga caagcagagc gauacccagu auguaugcaa aaggacuuug      840

guggaccggg gauggggcaa ugguuguggg cuguucggaa agggcagucu agucacaugc      900

gcgaaauuug ccugcucuaa gaaaaugacc ggcaagucca uucagccuga aaaccucgag      960

uaccguauca ugcugagcgu gcacgggucg caacacucug gaaugauugu uaaugauacg     1020

ggucaugaaa ccgacgagaa ccgcgcaaag guggagauca caccaaauuc ccccagagcu     1080

gaagccacuc uuggcggguu cggaucacug ggcuuggauu gugagccuag gaccggucuc     1140

gacuuuagug aucuguauua cuuaacaaug aacaauaaac acuggcuggu ccauaaggaa     1200

ugguuccacg acaucccacu cccguggcac gcuggggccg auacuggcac cccucauugg     1260

aacaauaaag aggcacuugu ggaauuuaag gacgcccacg cuaaacggca gacaguaguu     1320

gugcugggaa gccaggaggg cgcgguccau accgcauugg ccggggcucu agaggccgaa     1380

auggauggag caaagggucg acuguccucu ggccaccuca aaugccgccu gaagauggac     1440

aaacuuagac ugaagggggu gagcuauuca uuguguacug ccgcuuucac guuuacaaag     1500

auuccugccg agacccucca uggaacuguc accguggaag uucaguacgc aggcacagac     1560

gguccaugca aagugcccgc gcaaauggcu guagauaugc agacccugac uccugucggg     1620

agguuaauaa cagccaaccc agugaucacg gagaguaccg aaaacuccaa gaugaugcug     1680

gagcuagauc ccccguucgg cgacagcuac auuguuaucg gagugggcga gaaaaagaua     1740

acucaccacu ggcaucgguc ugggucaaca auuggaaagg cauuugaagc caccguccgc     1800

ggugcuaaaa gaauggccgu gcucggcgac acagcauggg auuucgggag uguaggaggc     1860

gcucugaaua gccuugguaa ggggauccac cagaucuucg gcgccgcguu uaaauccuug     1920

uucggaggca ugucuugguu uucgcaaauu cugaucggga cucuccugau guggcuugga     1980

cugaacacca agaauggcag cauuuccuug augugucucg cccugggugg gguguuaaua     2040

uuccugucaa cggcuguuag ugcagac                                         2067


<210>  1715
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1715
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc agau                                            2064


<210>  1716
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1716
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcagc cguuacgaga       60

aggggauccg cguacuauau guaccuggac cggaacgaug cuggugaggc cauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugcaacua ugucauacga augcccuaug cuggacgagg gaguggaacc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaggccc gccgaagccg uagagcugug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaauca cgcgaguaca ccaagcaccu uauuagaguu      420

gagaauugga ucuuuaggaa ccccggguuc gcacuggccg cugccgcgau ugcaugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcuuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaagg cauguccggg      600

ggaacuuggg uugauauugu guuagagcau gguggcugug ucaccgugau ggcccaggac      660

aagccaacag uggauaucga acuggucacc acgacuguuu caaacauggc agaggugcga      720

aguuacugcu augaggcuag cauaucugac auggccuccg auucaagaug ucccacacaa      780

ggggaagcgu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcc      900

aaauuugcau gcucuaagaa aaugaccggc aaguccauuc agccugagaa ccucgaauac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugagaccg augaaaaccg cgcuaaggug gagaucacac caaauucccc cagagccgag     1080

gcuacucuug gcggguucgg aucacugggc uuggacugug aaccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggagugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcagaca cuggcacacc ccauuggaac     1260

aauaaagaag cccuugugga guuuaaggau gcucacgcga aacggcagac cguaguugug     1320

cugggaagcc aggaaggcgc aguccauacc gccuuggcug gggcccuaga ggcagagaug     1380

gacggagcca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugcug ccuucacguu uacaaagauu     1500

ccugcagaaa cccuccaugg aacugucacc guggagguuc aguacgcggg cacagacggu     1560

ccaugcaaag ugcccgcuca aauggccgua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag caaacccagu gaucacggaa aguaccgaga acuccaagau gaugcuggaa     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggccu uugaggcuac cguccgcggu     1800

gccaaaagaa uggcagugcu cggcgauaca gcuugggacu ucgggagugu aggaggcgcc     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg cggccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgcuc uggguggggu guuaauauuc     2040

cugucaacgg caguuagugc cgau                                            2064


<210>  1717
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1717
agaggcgccg acaccagcgu ggggaucguc ggacugcucc uuacaacugc uauggcagcc       60

gcgguuacga ggcgggguuc cgcuuacuau auguaccugg aucgcaacga cgccggcgag      120

gcaauuucuu uccccaccac auuggggaug aauaagugcu auauacagau cauggauuua      180

ggacacaugu gugacgccac uaugucauac gaaugcccua ugcuggauga gggcguggaa      240

ccagacgaug uagacuguug gugcaacacc acaaguaccu ggguggucua ugguacuugu      300

caucacaaaa agggggaggc ucgacguagc agaagggcag ugacacuacc gucccauucu      360

acccggaaac uccaaacgcg cucgcagacu uggcuggaau caagagagua caccaagcac      420

cuuauuaggg uugagaauug gaucuuucgg aaccccggau ucgcccuggc ugccgcggca      480

auugccuggu ugcucggcag caguacaucc cagaaaguga ucuaucuggu caugauacug      540

cucaucgcuc cugccuacuc uauucgcugc aucggcguga gcaaucgaga uuuuguagaa      600

gggauguccg gagguacuug gguugacauu guguuagagc auggcgggug ugucaccgug      660

auggcacagg auaagccaac aguggacauc gaacugguca ccacgacugu uucaaacaug      720

gcugagguga gaaguuacug cuaugaggcc agcauaucug auauggcguc cgacucaagg      780

ugucccacac aaggagaagc cuaccuugac aagcagagcg auacccagua uguaugcaaa      840

cggacuuugg uggaccgugg cugggguaau ggguguggac uguucggcaa gggcagucua      900

gucacaugcg caaaauuugc uugcucuaag aaaaugaccg ggaaguccau ucagccugag      960

aaccucgaau accgcaucau gcugagcgug cacggaucgc aacacucugg uaugauuguu     1020

aaugauacgg gccaugagac cgacgaaaac agagccaagg uggagaucac accaaauucc     1080

cccagggcug aggccacucu ugggggauuc ggcucacugg guuuggauug ugaaccucgg     1140

accgggcucg acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc     1200

cauaaggagu gguuccacga caucccacuc ccguggcacg caggcgccga uacuggaaca     1260

ccccauugga acaauaaaga agcucuugug gaguuuaagg acgcgcacgc aaaacgacag     1320

accguaguug ugcugggcag ccaggaaggg gccguccaua ccgcuuuggc cggagcacua     1380

gaggccgaga uggauggugc uaagggccgc cuguccucug ggcaccucaa augcagacug     1440

aagauggaca agcugaggcu gaaaggagug agcuauucau uguguacugc cgcauucacg     1500

uuuacaaaga uuccugcgga aacccuccau ggcacuguca ccguggaggu ucaguacgcu     1560

gguacagacg ggccaugcaa agugcccgcc caaauggcag uagauaugca gacccugacu     1620

ccugucggcc gguuaauaac agccaaccca gugaucacgg aaaguaccga gaacuccaag     1680

augaugcugg aacuagaucc cccguucgga gacagcuaca uuguuaucgg cgugggggag     1740

aaaaagauaa cucaccacug gcaucgcucu ggaucaacaa uugguaaggc uuuugaggcc     1800

accgucagag gcgcaaaaag gauggcugug cucggggaca cagccuggga uuucggaagu     1860

guaggcggug cgcugaauag ccuugggaag ggcauccacc agaucuucgg agccgcuuuu     1920

aaauccuugu ucggcgggau gucuugguuu ucgcaaauuc ugaucggaac ucuccugaug     1980

uggcuuggcc ugaacaccaa gaaugguagc auuuccuuga ugugucucgc acuuggggga     2040

guguuaauau uccugucaac ggccguuagu gcugac                               2076


<210>  1718
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1718
agaggccuga gcuccaggaa gcggcgcucu cacgacgugc ucaccgucca guuccuuauc       60

cuggggaugu uguuaaugac aggagguguu acucgacgug gcucagccua cuauauguac      120

cuggauagaa acgacgcugg ggaggcaauu aguuuuccca cgacccuagg aaugaauaaa      180

ugcuauauac aaaucaugga ucucggccau augugugacg ccacaaugag cuacgaaugc      240

ccuaugcugg augagggugu ggaaccagac gauguagacu guuggugcaa cacuaccucc      300

acaugggugg ucuaugggac cugucaccau aagaaaggag aggcgaggcg gucucgcaga      360

gcugugacuc uuccgucgca cucaacaagg aagcugcaga cccggagcca gacgugguug      420

gaaagucgcg aguacacuaa acaucucauu cgaguugaga auuggaucuu cagaaacccc      480

ggcuuugccc uggcagccgc ugcaauugcc uggcugcucg gcuccucuac cagccagaag      540

gugaucuauu uagucaugau acugcuuauc gcuccugccu acuccauuag gugcaucggg      600

gugucaaauc gggauuucgu agaaggaaug agugguggca cauggguuga cauuguguug      660

gagcacgggg gaugugucac ugugauggcg caagauaagc caaccgugga caucgaacug      720

gucacaacca cgguuagcaa cauggcagag gugcguucuu acugcuauga ggccuccaua      780

ucagauaugg cuagcgacag ucgcuguccc acucagggcg aagccuaccu agacaaacag      840

ucugauacac aguauguaug caagagaacc cucguggaca gggguugggg gaauggaugu      900

ggccuguuug gcaaaggguc ccuugucacu ugcgcaaagu ucgcuugcag caaaaagaug      960

acaggaaagu cgauucaacc ugagaaccug gaauaccgga ucauguuguc ugugcacggu     1020

ucccagcauu caggcaugau uguuaaugau accgggcacg agacggacga aaaccgagcc     1080

aaaguggaga ucaccccaaa uaguccccgc gcggaggcca cacucggagg cuuugguagc     1140

cugggguuag auugugaacc uagaacuggc cuggacuucu ccgaucucua uuaccuuacc     1200

augaacaaua agcauuggcu gguccacaaa gagugguuuc acgacauccc auugccgugg     1260

caugcaggag cugauacagg cacuccccac uggaacaaua aggaagcccu aguggaguuc     1320

aaagacgcuc augccaagag gcagaccgua guugugcugg ggucucagga aggagcaguc     1380

cacacagccc ucgcuggugc gcuggaggca gagauggaug gcgccaaagg gcggcuuagc     1440

ucaggacauc ugaagugccg cuugaagaug gacaaacuca gacugaaggg cgugaguuau     1500

uccuuaugua ccgcugccuu uacuuucacg aaaauuccug cagaaacacu gcacgguacc     1560

gucacugugg agguucaaua cgccgggacc gacggcccau gcaaggugcc cgcucagaug     1620

gccguagaua ugcagacacu aaccccuguc ggaaggcuca uaacugcaaa cccagugauc     1680

acagaaagca cggagaacuc uaaaaugaug cuggaacuug aucccccguu uggcgacuca     1740

uacauuguua ucgggguggg agagaagaag auaacccacc auuggcaccg uagugguagc     1800

acuauuggca aagcguucga ggcuacaguc cgaggggcca agcggauggc aguguuggga     1860

gacaccgccu gggauuucgg cuccguaggu ggggcucuca auucucuggg caaaggaauc     1920

caucaaaucu uuggcgccgc auucaagucg cuguuugggg gaaugagcug guucucccag     1980

auucuuaucg gcacacuguu gauguggcuc ggucugaaca cuaaaaaugg gucaauuagu     2040

uuaauguguc uggcucuagg aggcgugcuc auauuucugu cuaccgccgu uagcgcggac     2100


<210>  1719
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1719
agaggccuga gcuccaggaa gcggcgcucu cacgacgugc ucaccgucca guuccuuauc       60

cuggggaugu uguuaaugac aggagccguu acucgacgug guucagcuua cuauauguac      120

cuggauagaa acgacgcagg cgaggccauu aguuuuccca cgacccuagg gaugaauaaa      180

ugcuauauac aaaucaugga ucucggacau augugugacg cgacaaugag cuacgaaugc      240

ccuaugcugg augagggcgu ggaaccagac gauguagacu guuggugcaa cacuaccucc      300

acaugggugg ucuaugguac cugucaccau aagaaagggg aggcuaggcg gucucgcaga      360

gccgugacuc uuccgucgca cucaacaagg aagcugcaga cccggagcca gacgugguug      420

gaaagucgcg aguacacuaa acaucucauu cgaguugaga auuggaucuu cagaaacccc      480

ggauuugcac uggccgcugc agccauugcu uggcugcucg gcuccucuac cagccagaag      540

gugaucuauu uagucaugau acugcuuauc gccccugcgu acuccauuag gugcaucggc      600

gugucaaauc gggauuucgu agaagggaug aguggaggua cauggguuga cauuguguug      660

gagcacggcg ggugugucac ugugauggca caagauaagc caaccgugga caucgaacug      720

gucacaacca cgguuagcaa cauggccgag gugcguucuu acugcuauga ggcuuccaua      780

ucagauaugg ccagcgacag ucgcuguccc acucagggag aagcauaccu agacaaacag      840

ucugauacac aguauguaug caagagaacc cucguggaca ggggcugggg uaaugggugu      900

ggacuguuug gcaaaggcuc ccuugucacu ugcgcuaagu ucgccugcag caaaaagaug      960

acagggaagu cgauucaacc ugagaaccug gaauaccgga ucauguuguc ugugcacgga     1020

ucccagcauu cagguaugau uguuaaugau accggccacg agacggacga aaaccgagcg     1080

aaaguggaga ucaccccaaa uaguccccgc gccgaggcaa cacucggggg auuuggcagc     1140

cuggguuuag auugugaacc uagaacuggg cuggacuucu ccgaucucua uuaccuuacc     1200

augaacaaua agcauuggcu gguccacaaa gagugguuuc acgacauccc auugccgugg     1260

caugcuggcg ccgauacagg aacuccccac uggaacaaua aggaagcucu aguggaguuc     1320

aaagacgccc augcaaagag gcagaccgua guugugcugg gcucucagga aggggccguc     1380

cacacagcuc ucgcgggagc acuggaggcc gagauggaug gugcuaaagg ccggcuuagc     1440

ucagggcauc ugaagugccg cuugaagaug gacaaacuca gacugaaggg agugaguuau     1500

uccuuaugua ccgccgcauu uacuuucacg aaaauuccug ccgaaacacu gcacggcacc     1560

gucacugugg agguucaaua cgcugguacc gacgggccau gcaaggugcc cgcccagaug     1620

gcaguagaua ugcagacacu aaccccuguc ggcaggcuca uaacugcgaa cccagugauc     1680

acagaaagca cggagaacuc uaaaaugaug cuggaacuug aucccccguu uggagacuca     1740

uacauuguua ucggcguggg ggagaagaag auaacccacc auuggcaccg uaguggaagc     1800

acuauuggua aggcuuucga ggccacaguc cggggcgcaa aacgaauggc cguguugggg     1860

gacaccgcuu gggauuucgg auccguaggc ggugcccuca auucucuggg gaaaggcauc     1920

caucaaaucu uuggagcagc uuucaagucg cuguuuggcg ggaugagcug guucucccag     1980

auucuuaucg gaacacuguu gauguggcuc ggccugaaca cuaaaaaugg uucaauuagu     2040

uuaauguguc uggcccuagg gggagugcuc auauuucugu cuaccgcggu uagcgccgac     2100


<210>  1720
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1720
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

auaguguuag agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac      720

auugagcugg ucaccacgac uguuucaaau auggcugaag ugcguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aaguggaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guggaguuua aagacgcgca cgcaaagagg cagaccguag uugugcuggg cagccaggag     1380

ggggccgucc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagacccug acuccugucg gcagguuaau uacagccaac     1680

ccagugauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggagugcuga uuuuccuguc aacggccguu     2100

agugcu                                                                2106


<210>  1721
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1721
aucaacgcca gaaaggagaa aaagaggcgg ggcgcugaca ccagcguggg gauugucgga       60

cugcuccuua caacugcaau ggccgcggaa guuacgcgcc gagguuccgc uuacuauaug      120

uaccuggauc guaaugacgc cggcgaggca auaucuuucc ccaccacauu ggggaugaac      180

aaaugcuaua uccagauuau ggauuuagga cacaugugug acgccacuau gucauacgaa      240

ugcccuaugc uggaugaggg cguggaacca gacgauguag acuguuggug caauaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaagaaag gggaggcuag aaggagccgg      360

cgcgcaguga cacuaccguc ccauucuacc agaaagcucc aaacgagguc gcagacuugg      420

cuggagucac gggaauacac caagcaccuu auccgcguug agaacuggau uuuucgaaau      480

cccggauucg cccuggcugc cgcggcaauc gccugguugc ucggcagcag uacaucccag      540

aaagugauau aucuggucau gauccugcuc auugcuccug ccuacucuau cagaugcauu      600

ggcgugagca acagggauuu uguagaaggg auguccggag guacuugggu ugacaucgug      660

uuagagcaug gcgggugugu caccgugaug gcacaggaua agccaacagu ggacauagag      720

cuggucacca cgacuguuuc aaauauggcu gaagugcgga guuacugcua ugaggccagc      780

auuucugaua uggcguccga cucacguugu cccacacaag gagaagccua ccuugacaaa      840

cagagcgaua cccaguaugu augcaagcgc acuuuggugg acagaggcug ggguaacggg      900

uguggacugu ucggcaaagg cagucuaguc acaugcgcaa aguuugcuug cucuaagaaa      960

augaccggga aguccaucca gccugagaau cucgaauaca ggauuaugcu gagcgugcac     1020

ggaucgcaac acucugguau gaucguuaac gauacgggcc augagaccga cgagaaucgg     1080

gccaaagugg aaaucacacc aaacucaccc cgagcugagg ccacucuugg gggauucggc     1140

ucccuggguu uggauuguga accucgcacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaua acaagcacug gcugguccau aaagaguggu uccacgacau uccacucccg     1260

uggcacgcag gcgccgauac uggaacaccc cauuggaaua acaaggaagc ucuuguggag     1320

uuuaaagacg cgcacgcaaa gagacagacc guaguugugc ugggcagcca ggagggggcc     1380

guccauaccg cuuuggccgg agcacuagaa gccgagaugg auggugcuaa gggcaggcug     1440

uccucugggc accucaaaug ccggcugaag auggacaaac uucgccugaa gggagugagc     1500

uauucauugu guacugccgc auucacguuu acaaaaauac cugcggaaac ccuccauggc     1560

acugucaccg uggagguuca guacgcuggu acagacgggc caugcaaggu gcccgcccaa     1620

auggcaguag auaugcagac ccugacuccu gucggcagau uaaucacagc caacccagug     1680

auuacggaaa guaccgaaaa uuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacaucg uuauaggcgu gggggagaaa aagauuacuc accacuggca uaggucugga     1800

ucaacaaucg guaaggcuuu ugaggccacc guccguggcg caaaacggau ggcugugcuc     1860

ggggacacag ccugggauuu cggaagugua ggcggugcgc ugaacagccu ugggaaaggc     1920

auccaccaga uuuucggagc cgcuuuuaag uccuuguucg gcgggauguc uugguuuucg     1980

caaauccuga uuggaacucu ccugaugugg cuuggccuga auaccaagaa cgguagcaua     2040

uccuugaugu gucucgcauu agggggagug cugaucuucc ugucaacggc cguuagugcu     2100


<210>  1722
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1722
aucauuaacg ccagaaagga gaaaaagagg cggggcgcug acaccagcgu ggggauaguc       60

ggacugcucc uuacaacugc aauggccgcg gaaguuacgc gccgagguuc cgcuuacuau      120

auguaccugg aucguaauga cgccggcgag gcaaucucuu uccccaccac auuggggaug      180

aacaaaugcu auauucagau cauggauuua ggacacaugu gugacgccac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaauacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaga aaggggaggc uagaaggagc      360

cggcgcgcag ugacacuacc gucccauucu accagaaagc uccaaacgag gucgcagacu      420

uggcuggagu cacgggaaua caccaagcac cuuauucgcg uugagaacug gaucuuucga      480

aaucccggau ucgcccuggc ugccgcggca auagccuggu ugcucggcag caguacaucc      540

cagaaaguga ucuaucuggu caugauucug cucaucgcuc cugccuacuc uauuagaugc      600

aucggcguga gcaacaggga uuuuguagaa gggauguccg gagguacuug gguugacaua      660

guguuagagc auggcgggug ugucaccgug auggcacagg auaagccaac aguggacauu      720

gagcugguca ccacgacugu uucaaauaug gcugaagugc ggaguuacug cuaugaggcc      780

agcaucucug auauggcguc cgacucacgu ugucccacac aaggagaagc cuaccuugac      840

aaacagagcg auacccagua uguaugcaag cgcacuuugg uggacagagg cugggguaac      900

ggguguggac uguucggcaa aggcagucua gucacaugcg caaaguuugc uugcucuaag      960

aaaaugaccg ggaaguccau ucagccugag aaucucgaau acaggaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugaucguu aacgauacgg gccaugagac cgacgagaau     1080

cgggccaaag uggaaauuac accaaacuca ccccgagcug aggccacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucgc accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga auaacaagca cuggcugguc cauaaagagu gguuccacga cauaccacuc     1260

ccguggcacg caggcgccga uacuggaaca ccccauugga auaacaagga agcucuugug     1320

gaguuuaaag acgcgcacgc aaagagacag accguaguug ugcugggcag ccaggagggg     1380

gccguccaua ccgcuuuggc cggagcacua gaagccgaga uggauggugc uaagggcagg     1440

cuguccucug ggcaccucaa augccggcug aagauggaca aacuucgccu gaagggagug     1500

agcuauucau uguguacugc cgcauucacg uuuacaaaaa ucccugcgga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcu gguacagacg ggccaugcaa ggugcccgcc     1620

caaauggcag uagauaugca gacccugacu ccugucggca gauuaauuac agccaaccca     1680

gugaucacgg aaaguaccga aaauuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uaguuauugg cgugggggag aaaaagauca cucaccacug gcauaggucu     1800

ggaucaacaa ucgguaaggc uuuugaggcc accguccgug gcgcaaaacg gauggcugug     1860

cucggggaca cagccuggga uuucggaagu guaggcggug cgcugaacag ccuugggaaa     1920

ggcauucacc agaucuucgg agccgcuuuu aaguccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugauaggaac ucuccugaug uggcuuggcc ugaauaccaa gaacgguagc     2040

aucuccuuga ugugucucgc auuaggggga gugcugauuu uccugucaac ggccguuagu     2100

gcu                                                                   2103


<210>  1723
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1723
aacgccagaa aggagaaaaa gaggcggggc gcugacacca gcguggggau cgucggacug       60

cuccuuacaa cugcaauggc cgcggaaguu acgcgccgag guuccgcuua cuauauguac      120

cuggaucgua augacgccgg cgaggcaauu ucuuucccca ccacauuggg gaugaacaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg ccacuauguc auacgaaugc      240

ccuaugcugg augagggcgu ggaaccagac gauguagacu guuggugcaa uaccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aggcuagaag gagccggcgc      360

gcagugacac uaccguccca uucuaccaga aagcuccaaa cgaggucgca gacuuggcug      420

gagucacggg aauacaccaa gcaccuuauu cgcguugaga acuggaucuu ucgaaauccc      480

ggauucgccc uggcugccgc ggcaauugcc ugguugcucg gcagcaguac aucccagaaa      540

gugaucuauc uggucaugau acugcucauc gcuccugccu acucuauuag augcaucggc      600

gugagcaaca gggauuuugu agaagggaug uccggaggua cuuggguuga cauuguguua      660

gagcauggcg ggugugucac cgugauggca caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa uauggcugaa gugcggaguu acugcuauga ggccagcaua      780

ucugauaugg cguccgacuc acguuguccc acacaaggag aagccuaccu ugacaaacag      840

agcgauaccc aguauguaug caagcgcacu uugguggaca gaggcugggg uaacgggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgcaaagu uugcuugcuc uaagaaaaug      960

accgggaagu ccauucagcc ugagaaucuc gaauacagga ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaacgau acgggccaug agaccgacga gaaucgggcc     1080

aaaguggaaa ucacaccaaa cucaccccga gcugaggcca cucuuggggg auucggcucc     1140

cuggguuugg auugugaacc ucgcaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaauaaca agcacuggcu gguccauaaa gagugguucc acgacauccc acucccgugg     1260

cacgcaggcg ccgauacugg aacaccccau uggaauaaca aggaagcucu uguggaguuu     1320

aaagacgcgc acgcaaagag acagaccgua guugugcugg gcagccagga gggggccguc     1380

cauaccgcuu uggccggagc acuagaagcc gagauggaug gugcuaaggg caggcugucc     1440

ucugggcacc ucaaaugccg gcugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucauugugua cugccgcauu cacguuuaca aaaauuccug cggaaacccu ccauggcacu     1560

gucaccgugg agguucagua cgcugguaca gacgggccau gcaaggugcc cgcccaaaug     1620

gcaguagaua ugcagacccu gacuccuguc ggcagauuaa uaacagccaa cccagugauc     1680

acggaaagua ccgaaaauuc caagaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaaaaag auaacucacc acuggcauag gucuggauca     1800

acaauuggua aggcuuuuga ggccaccguc cguggcgcaa aacggauggc ugugcucggg     1860

gacacagccu gggauuucgg aaguguaggc ggugcgcuga acagccuugg gaaaggcauc     1920

caccagaucu ucggagccgc uuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaua ccaagaacgg uagcauuucc     2040

uugauguguc ucgcauuagg gggagugcug auauuccugu caacggccgu uagugcu        2097


<210>  1724
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1724
gccagaaagg agaaaaagag gcggggcgcu gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug caauggccgc ggaaguuacg cgccgagguu ccgcuuacua uauguaccug      120

gaucguaacg acgccggcga ggcaauuucu uuccccacca cauuggggau gaauaaaugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcca cuaugucaua cgaaugcccu      240

augcuggaug agggcgugga accagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaag aaaggggagg cuagaaggag ccggcgcgca      360

gugacacuac cgucccauuc uaccagaaag cuccaaacga ggucgcagac uuggcuggag      420

ucacgggaau acaccaagca ccuuauucgc guugagaauu ggaucuuucg aaaccccgga      480

uucgcccugg cugccgcggc aauugccugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcu ccugccuacu cuauuagaug caucggcgug      600

agcaauaggg auuuuguaga agggaugucc ggagguacuu ggguugacau uguguuagag      660

cauggcgggu gugucaccgu gauggcacag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcugaagug cggaguuacu gcuaugaggc cagcauaucu      780

gauauggcgu ccgacucacg uugucccaca caaggagaag ccuaccuuga caaacagagc      840

gauacccagu auguaugcaa gcgcacuuug guggacagag gcugggguaa ugggugugga      900

cuguucggca aaggcagucu agucacaugc gcaaaguuug cuugcucuaa gaaaaugacc      960

gggaagucca uucagccuga gaaccucgaa uacaggauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaga ccgacgagaa ccgggccaaa     1080

guggaaauca caccaaauuc accccgagcu gaggccacuc uugggggauu cggcucccug     1140

gguuuggauu gugaaccucg caccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaagc acuggcuggu ccauaaagag ugguuccacg acaucccacu cccguggcac     1260

gcaggcgccg auacuggaac accccauugg aacaauaagg aagcucuugu ggaguuuaaa     1320

gacgcgcacg caaagagaca gaccguaguu gugcugggca gccaggaggg ggccguccau     1380

accgcuuugg ccggagcacu agaagccgag auggauggug cuaagggcag gcuguccucu     1440

gggcaccuca aaugccggcu gaagauggac aaacuucgcc ugaagggagu gagcuauuca     1500

uuguguacug ccgcauucac guuuacaaaa auuccugcgg aaacccucca uggcacuguc     1560

accguggagg uucaguacgc ugguacagac gggccaugca aggugcccgc ccaaauggca     1620

guagauaugc agacccugac uccugucggc agauuaauaa cagccaaccc agugaucacg     1680

gaaaguaccg agaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagguc uggaucaaca     1800

auugguaagg cuuuugaagc caccguccgu ggcgcaaaac ggauggcugu gcucggggac     1860

acagccuggg auuucggaag uguaggcggu gcgcugaaua gccuugggaa aggcauccac     1920

cagaucuucg gagccgcuuu uaaguccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cauuaggggg agugcugaua uuccugucaa cggccguuag ugcu           2094


<210>  1725
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1725
agaaaggaga aaaagaggcg gggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga aguuacgcgc cgagguuccg cguacuauau guaccuggau      120

cguaacgacg cuggcgaggc cauuucuuuc cccaccacau uggggaugaa uaaaugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaagaaa ggggaggcca gaaggagccg gcgcgcugug      360

acacuaccgu cccauucuac cagaaagcuc caaacgaggu cgcagacuug gcuggaguca      420

cgggaauaca ccaagcaccu uauucgcguu gagaauugga ucuuucgaaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uuagaugcau cggcgugagc      600

aauagggauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaagugcgg aguuacugcu augaggcuag cauaucugau      780

auggccuccg acucacguug ucccacacaa ggagaagcgu accuugacaa acagagcgau      840

acccaguaug uaugcaagcg cacuuuggug gacagaggcu gggguaaugg guguggacug      900

uucggcaaag gcagucuagu cacaugcgcc aaguuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac aggaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgagaaccg ggcuaaagug     1080

gaaaucacac caaauucacc ccgagccgag gcuacucuug ggggauucgg cucccugggu     1140

uuggauugug aaccucgcac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaagcacu ggcuggucca uaaagagugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaggaag cccuugugga guuuaaagac     1320

gcucacgcga agagacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga agcagagaug gauggugcca agggcaggcu guccucuggg     1440

caccucaaau gccggcugaa gauggacaaa cuucgccuga agggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaaaauu ccugcagaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcggg uacagacggg ccaugcaagg ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggcaga uuaauaacag caaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc auaggucugg aucaacaauu     1800

gguaaagccu uugaagcuac cguccguggc gccaagcgga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaagg cauccaccag     1920

aucuucggag cggccuuuaa guccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuu uagggggagu gcugauauuc cugucaacgg caguuagugc c              2091


<210>  1726
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1726
aaggagaaaa agagaagggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaagu uacgcggcgc gguuccgcgu acuauaugua ccuggaucga      120

aacgacgcug gcgaggccau uucuuucccc accacauugg ggaugaauaa augcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgaaug cccuaugcug      240

gaugagggcg uggaaccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caagaaaggg gaggcccgua gaagcaggcg ggcugugaca      360

cuaccguccc auucuacccg caagcuccaa acgagaucgc agacuuggcu ggagucaagg      420

gaauacacca agcaccuuau ucggguugag aauuggaucu uucgcaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc gaugcaucgg cgugagcaau      600

agagauuuug uagaagggau guccggaggu acuuggguug acauuguguu agagcauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga agugaggagu uacugcuaug aggcuagcau aucugauaug      780

gccuccgacu cacggugucc cacacaagga gaagcguacc uugacaaaca gagcgauacc      840

caguauguau gcaagcguac uuugguggac cgcggcuggg guaaugggug uggacuguuc      900

ggcaaaggca gucuagucac augcgccaag uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugagaaccu cgaauacaga aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gagaccgacg agaacagggc uaaaguggaa     1080

aucacaccaa auucaccccg ggccgaggcu acucuugggg gauucggcuc ccuggguuug     1140

gauugugaac cucgaaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aagcacuggc ugguccauaa agagugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaggaagccc uuguggaguu uaaagacgcu     1320

cacgcgaagc gccagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaagc agagauggau ggugccaagg gcagacuguc cucugggcac     1440

cucaaaugca ggcugaagau ggacaaacuu cggcugaagg gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaaaauuccu gcagaaaccc uccauggcac ugucaccgug     1560

gagguucagu acgcggguac agacgggcca ugcaaggugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggccgcuua auaacagcaa acccagugau cacggaaagu     1680

accgagaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcaua gaucuggauc aacaauuggu     1800

aaagccuuug aagcuaccgu caggggcgcc aagcguaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaaaggcau ccaccagauc     1920

uucggagcgg ccuuuaaguc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcuuuag ggggagugcu gauauuccug ucaacggcag uuagugcc                  2088


<210>  1727
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1727
gagaagaaaa gaaggggcgc cgacaccagc guggggaucg ucggacugcu ccuuacaacu       60

gcuauggcag ccgaaguuac gcggcgcggu uccgcguacu auauguaccu ggaucgaaac      120

gacgcuggcg aggccauuuc uuuccccacc acauugggga ugaauaagug cuauauacag      180

aucauggauu uaggacacau gugugacgca acuaugucau acgaaugccc uaugcuggau      240

gagggcgugg aaccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uaugguacuu gucaucacaa aaagggggag gcccguagaa gcaggcgggc ugugacacua      360

ccgucccauu cuacccgcaa acuccaaacg agaucgcaga cuuggcugga gucaagggaa      420

uacaccaagc accuuauucg gguugagaau uggaucuuuc gcaaccccgg auucgcacug      480

gccgcugccg cgauugcaug guugcucggc agcaguacau cccagaaggu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucgau gcaucggcgu gagcaauaga      600

gauuuuguag aagggauguc cggagguacu uggguugaca uuguguuaga gcauggcggg      660

ugugucaccg ugauggccca ggauaaacca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaagu gaggaguuac ugcuaugagg cuagcauauc ugauauggcc      780

uccgacucac ggugucccac acaaggagaa gcguaccuug acaagcagag cgauacccag      840

uauguaugca aacguacuuu gguggaccgc ggcuggggua augggugugg acuguucggc      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaagaugac cgggaaaucc      960

auucagccug agaaccucga auacagaauc augcugagcg ugcacggauc gcaacacucu     1020

gguaugauug uuaaugauac gggccaugag accgacgaga acagggcuaa gguggaaauc     1080

acaccaaauu caccccgggc cgaggcuacu cuugggggau ucggcucccu ggguuuggau     1140

ugugaaccuc gaaccgggcu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga gugguuccac gacaucccac ucccguggca cgccggcgca     1260

gauacuggaa caccccauug gaacaauaaa gaagcccuug uggaguuuaa ggacgcucac     1320

gcgaaacgcc agaccguagu ugugcugggc agccaggagg gggcagucca uaccgccuug     1380

gcuggagccc uagaagcaga gauggauggu gccaagggca gacuguccuc ugggcaccuc     1440

aagugcaggc ugaaaaugga caagcugcgg cugaaaggag ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gaaacccucc auggcacugu caccguggag     1560

guucaguacg cggguacaga cgggccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg ccgcuuaaua acagcaaacc cagugaucac ggaaaguacc     1680

gagaacucca agaugaugcu ggagcuagau cccccguucg gagacagcua cauuguuauc     1740

ggcguggggg aaaagaaaau aacucaccac uggcauagau cuggaucaac aauugguaag     1800

gccuuugagg cuaccgucag gggcgccaaa cguauggcag ugcucgggga cacagcuugg     1860

gauuucggaa guguaggcgg ugcccugaau agccuuggga agggcaucca ccagaucuuc     1920

ggagcggccu uuaaauccuu guucggcggg augucuuggu uuucgcaaau ucugaucgga     1980

acucuccuga uguggcuugg ccugaacacc aagaauggua gcauuuccuu gaugugucuc     2040

gcucuugggg gaguguuaau auuccuguca acggcaguua gugcc                     2085


<210>  1728
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1728
aagaaaagaa ggggcgccga caccagcgug gggaucgucg gacugcuccu uacaacugcu       60

auggcagccg agguuacgcg gcgcgguucc gcguacuaua uguaccugga ucgaaacgac      120

gcuggcgaag ccauuucuuu ccccaccaca uuggggauga auaagugcua uauacagauc      180

auggauuuag gacacaugug ugacgcaacu augucauacg agugcccuau gcuggaugaa      240

ggcguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

gguacuuguc aucacaaaaa gggggaagcc cguagaagca ggcgggcugu gacacuaccg      360

ucccauucua cccgcaaacu ccaaacgaga ucgcagacuu ggcuggaguc aagggaguac      420

accaagcacc uuauucgggu ugaaaauugg aucuuucgca accccggauu cgcacuggcc      480

gcugccgcga uugcaugguu gcucggcagc aguacauccc agaaggugau cuaucugguc      540

augauacugc ucaucgcccc ugcuuacucu auucgaugca ucggcgugag caauagagau      600

uuuguagagg ggauguccgg agguacuugg guugacauug uguuagaaca uggcgggugu      660

gucaccguga uggcccagga uaaaccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg cagaggugag gaguuacugc uaugaagcua gcauaucuga uauggccucc      780

gacucacggu gucccacaca aggagaggcg uaccuugaca agcagagcga uacccaguau      840

guaugcaaac guacuuuggu ggaccgcggc ugggguaaug gguguggacu guucggcaag      900

ggcagucuag ucacaugcgc caaauuugca ugcucuaaga agaugaccgg gaaauccauu      960

cagccugaaa accucgagua cagaaucaug cugagcgugc acggaucgca acacucuggu     1020

augauuguua augauacggg ccaugaaacc gacgagaaca gggcuaaggu ggagaucaca     1080

ccaaauucac cccgggccga agcuacucuu gggggauucg gcucccuggg uuuggauugu     1140

gagccucgaa ccgggcucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc cggcgcagau     1260

acuggaacac cccauuggaa caauaaagag gcccuugugg aauuuaagga cgcucacgcg     1320

aaacgccaga ccguaguugu gcugggcagc caggaggggg caguccauac cgccuuggcu     1380

ggagcccuag aggcagaaau ggauggugcc aagggcagac uguccucugg gcaccucaag     1440

ugcaggcuga aaauggacaa gcugcggcug aaaggaguga gcuauucauu guguacugcu     1500

gccuucacgu uuacaaagau uccugcagag acccuccaug gcacugucac cguggaaguu     1560

caguacgcgg guacagacgg gccaugcaaa gugcccgcuc aaauggccgu agauaugcag     1620

acccugacuc cugucggccg cuuaauaaca gcaaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggag acagcuacau uguuaucggc     1740

gugggggaga agaaaauaac ucaccacugg cauagaucug gaucaacaau ugguaaggcc     1800

uuugaagcua ccgucagggg cgccaaacgu auggcagugc ucggggacac agcuugggau     1860

uucggaagug uaggcggugc ccugaauagc cuugggaagg gcauccacca gaucuucgga     1920

gcggccuuua aauccuuguu cggcgggaug ucuugguuuu cgcaaauucu gaucggaacu     1980

cuccugaugu ggcuuggccu gaacaccaag aaugguagca uuuccuugau gugucucgcu     2040

cuugggggag uguuaauauu ccugucaacg gcaguuagug cc                        2082


<210>  1729
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1729
aagagaaggg gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug       60

gcagccgagg uuacgcggcg cgguuccgcg uacuauaugu accuggaucg aaacgacgcu      120

ggcgaagcca uuucuuuccc caccacauug gggaugaaua aaugcuauau acagaucaug      180

gauuuaggac acauguguga cgcaacuaug ucauacgagu gcccuaugcu ggaugaaggc      240

guggagccag acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu      300

acuugucauc acaagaaagg ggaagcccgu agaagcaggc gggcugugac acuaccgucc      360

cauucuaccc gcaagcucca aacgagaucg cagacuuggc uggagucaag ggaguacacc      420

aaacaccuua uucggguuga aaauuggauc uuucgcaacc ccggauucgc acuggccgcu      480

gccgcgauug caugguugcu cggcagcagu acaucccaga aggugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cgaugcaucg gcgugagcaa uagagauuuu      600

guagagggga uguccggagg uacuuggguu gacauugugu uagaacaugg cggguguguc      660

accgugaugg cccaggauaa gccaacagug gacaucgagc uggucaccac gacuguuuca      720

aacauggcag aggugaggag uuacugcuau gaagcuagca uaucugauau ggccuccgac      780

ucacgguguc ccacacaagg agaggcguac cuugacaaac agagcgauac ccaguaugua      840

ugcaagcgua cuuuggugga ccgcggcugg gguaaugggu guggacuguu cggcaaaggc      900

agucuaguca caugcgccaa guuugcaugc ucuaaaaaga ugaccgggaa guccauucag      960

ccugaaaacc ucgaguacag aaucaugcug agcgugcacg gaucgcaaca cucugguaug     1020

auuguuaaug auacgggcca ugaaaccgac gagaacaggg cuaaagugga gaucacacca     1080

aauucacccc gggccgaagc uacucuuggg ggauucggcu cccuggguuu ggauugugag     1140

ccucgaaccg ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaagcacugg     1200

cugguccaua aagaaugguu ccacgacauc ccacucccgu ggcacgccgg cgcagauacu     1260

ggaacacccc auuggaacaa uaaggaggcc cuuguggaau uuaaagacgc ucacgcgaag     1320

cgccagaccg uaguugugcu gggcagccag gagggggcag uccauaccgc cuuggcugga     1380

gcccuagagg cagaaaugga uggugccaaa ggcagacugu ccucugggca ccucaagugc     1440

aggcugaaga uggacaaacu ucggcugaag ggagugagcu auucauugug uacugcugcc     1500

uucacguuua caaaaauucc ugcagagacc cuccauggca cugucaccgu ggaaguucag     1560

uacgcgggua cagacgggcc augcaaggug cccgcucaaa uggccguaga uaugcagacc     1620

cugacuccug ucggccgcuu aauaacagca aacccaguga ucacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggagaca gcuacauugu uaucggcgug     1740

ggggagaaga agauaacuca ccacuggcau agaucuggau caacaauugg uaaagccuuu     1800

gaagcuaccg ucaggggcgc caagcguaug gcagugcucg gggacacagc uugggauuuc     1860

ggaaguguag gcggugcccu gaauagccuu gggaaaggca uccaccagau cuucggagcg     1920

gccuuuaagu ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc     1980

cugauguggc uuggccugaa caccaaaaau gguagcauuu ccuugaugug ucucgcuuua     2040

gggggagugc ugauauuccu gucaacggca guuagugcc                            2079


<210>  1730
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1730
agaaggggcg ccgacaccag cguggggauc gucggacugc uccuuacaac ugcuauggca       60

gccgagguua cgcggcgcgg uuccgcguac uauauguacc uggaucgaaa cgacgcuggc      120

gaagccauuu cuuuccccac cacauugggg augaauaagu gcuauauaca gaucauggau      180

uuaggacaca ugugugacgc aacuauguca uacgagugcc cuaugcugga ugaaggcgug      240

gagccagacg auguagacug uuggugcaac accacaagua ccuggguggu cuaugguacu      300

ugucaucaca aaaaggggga agcccguaga agcaggcggg cugugacacu accgucccau      360

ucuacccgca aacuccaaac gagaucgcag acuuggcugg agucaaggga guacaccaag      420

caccuuauuc ggguugaaaa uuggaucuuu cgcaaccccg gauucgcacu ggccgcugcc      480

gcgauugcau gguugcucgg cagcaguaca ucccagaaag ugaucuaucu ggucaugaua      540

cugcucaucg ccccugcuua cucuauucga ugcaucggcg ugagcaauag agauuuugua      600

gaggggaugu ccggagguac uuggguugac auuguguuag aacauggcgg gugugucacc      660

gugauggccc aggauaagcc aacaguggac aucgagcugg ucaccacgac uguuucaaac      720

auggcagagg ugaggaguua cugcuaugaa gcuagcauau cugauauggc cuccgacuca      780

cgguguccca cacaaggaga ggcguaccuu gacaagcaga gcgauaccca guauguaugc      840

aaacguacuu ugguggaccg cggcuggggu aaugggugug gacuguucgg caagggcagu      900

cuagucacau gcgccaaauu ugcaugcucu aagaaaauga ccgggaaguc cauucagccu      960

gaaaaccucg aguacagaau caugcugagc gugcacggau cgcaacacuc ugguaugauu     1020

guuaaugaua cgggccauga aaccgacgag aacagggcua agguggagau cacaccaaau     1080

ucaccccggg ccgaagcuac ucuuggggga uucggcuccc uggguuugga uugugagccu     1140

cgaaccgggc ucgacuuuag ugaucuguau uacuuaacaa ugaacaauaa acacuggcug     1200

guccauaagg aaugguucca cgacauccca cucccguggc acgccggcgc agauacugga     1260

acaccccauu ggaacaauaa agaggcccuu guggaauuua aggacgcuca cgcgaaacgc     1320

cagaccguag uugugcuggg cagccaggag ggggcagucc auaccgccuu ggcuggagcc     1380

cuagaggcag aaauggaugg ugccaagggc agacuguccu cugggcaccu caaaugcagg     1440

cugaagaugg acaagcugcg gcugaaagga gugagcuauu cauuguguac ugcugccuuc     1500

acguuuacaa agauuccugc agagacccuc cauggcacug ucaccgugga aguucaguac     1560

gcggguacag acgggccaug caaagugccc gcucaaaugg ccguagauau gcagacccug     1620

acuccugucg gccgcuuaau aacagcaaac ccagugauca cggagaguac cgaaaacucc     1680

aagaugaugc uggagcuaga ucccccguuc ggagacagcu acauuguuau cggcgugggg     1740

gagaaaaaga uaacucacca cuggcauaga ucuggaucaa caauugguaa ggccuuugaa     1800

gcuaccguca ggggcgccaa acguauggca gugcucgggg acacagcuug ggauuucgga     1860

aguguaggcg gugcccugaa uagccuuggg aagggcaucc accagaucuu cggagcggcc     1920

uuuaaauccu uguucggcgg gaugucuugg uuuucgcaaa uucugaucgg aacucuccug     1980

auguggcuug gccugaacac caagaauggu agcauuuccu ugaugugucu cgcucuuggg     2040

ggaguguuaa uauuccuguc aacggcaguu agugcc                               2076


<210>  1731
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1731
agaggcgccg acaccagcgu ggggaucguc ggacugcucc uuacaacugc uauggcagcc       60

gagguuacga ggcgggguuc cgcguacuau auguaccugg aucgcaacga cgcuggcgaa      120

gccauuucuu uccccaccac auuggggaug aauaagugcu auauacagau cauggauuua      180

ggacacaugu gugacgcaac uaugucauac gagugcccua ugcuggauga aggcguggag      240

ccagacgaug uagacuguug gugcaacacc acaaguaccu ggguggucua ugguacuugu      300

caucacaaaa agggggaagc ccgacguagc agaagggcug ugacacuacc gucccauucu      360

acccggaaac uccaaacgcg cucgcagacu uggcuggagu caagagagua caccaagcac      420

cuuauuaggg uugaaaauug gaucuuucgg aaccccggau ucgcacuggc cgcugccgcg      480

auugcauggu ugcucggcag caguacaucc cagaaaguga ucuaucuggu caugauacug      540

cucaucgccc cugcuuacuc uauucgcugc aucggcguga gcaaucgaga uuuuguagag      600

gggauguccg gagguacuug gguugacauu guguuagaac auggcgggug ugucaccgug      660

auggcccagg auaagccaac aguggacauc gagcugguca ccacgacugu uucaaacaug      720

gcagagguga gaaguuacug cuaugaagcu agcauaucug auauggccuc cgacucaagg      780

ugucccacac aaggagaggc guaccuugac aagcagagcg auacccagua uguaugcaaa      840

cggacuuugg uggaccgugg cugggguaau ggguguggac uguucggcaa gggcagucua      900

gucacaugcg ccaaauuugc augcucuaag aaaaugaccg ggaaguccau ucagccugaa      960

aaccucgagu accgcaucau gcugagcgug cacggaucgc aacacucugg uaugauuguu     1020

aaugauacgg gccaugaaac cgacgagaac agagcuaagg uggagaucac accaaauucc     1080

cccagggccg aagcuacucu ugggggauuc ggcucacugg guuuggauug ugagccucgg     1140

accgggcucg acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc     1200

cauaaggaau gguuccacga caucccacuc ccguggcacg ccggcgcaga uacuggaaca     1260

ccccauugga acaauaaaga ggcccuugug gaauuuaagg acgcucacgc gaaacgacag     1320

accguaguug ugcugggcag ccaggagggg gcaguccaua ccgccuuggc uggagcccua     1380

gaggcagaaa uggauggugc caagggccgc cuguccucug ggcaccucaa augcagacug     1440

aagauggaca agcugaggcu gaaaggagug agcuauucau uguguacugc ugccuucacg     1500

uuuacaaaga uuccugcaga gacccuccau ggcacuguca ccguggaagu ucaguacgcg     1560

gguacagacg ggccaugcaa agugcccgcu caaauggccg uagauaugca gacccugacu     1620

ccugucggcc gguuaauaac agcaaaccca gugaucacgg agaguaccga aaacuccaag     1680

augaugcugg agcuagaucc cccguucgga gacagcuaca uuguuaucgg cgugggggag     1740

aaaaagauaa cucaccacug gcaucgcucu ggaucaacaa uugguaaggc cuuugaagcu     1800

accgucagag gcgccaaaag gauggcagug cucggggaca cagcuuggga uuucggaagu     1860

guaggcggug cccugaauag ccuugggaag ggcauccacc agaucuucgg agcggccuuu     1920

aaauccuugu ucggcgggau gucuugguuu ucgcaaauuc ugaucggaac ucuccugaug     1980

uggcuuggcc ugaacaccaa gaaugguagc auuuccuuga ugugucucgc ucuuggggga     2040

guguuaauau uccugucaac ggcaguuagu gcc                                  2073


<210>  1732
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1732
ggcgccgaca ccagcguggg gaucgucgga cugcuccuua caacugcuau ggcagccgag       60

guuacgagaa gggguuccgc guacuauaug uaccuggauc ggaacgacgc uggcgaagcc      120

auuucuuucc ccaccacauu ggggaugaau aagugcuaua uacagaucau ggauuuagga      180

cacaugugug acgcaacuau gucauacgag ugcccuaugc uggaugaagg cguggagcca      240

gacgauguag acuguuggug caacaccaca aguaccuggg uggucuaugg uacuugucau      300

cacaaaaagg gggaagcccg ccgaagccgu agagcuguga cacuaccguc ccauucuacc      360

aggaaacucc aaacgcgguc gcagacuugg cuggagucac gcgaguacac caagcaccuu      420

auuagaguug aaaauuggau cuuuaggaac cccggauucg cacuggccgc ugccgcgauu      480

gcaugguugc ucggcagcag uacaucccag aaagugaucu aucuggucau gauacugcuc      540

aucgccccug cuuacucuau ucggugcauc ggcgugagca aucgcgauuu uguagagggg      600

auguccggag guacuugggu ugacauugug uuagaacaug gcgggugugu caccgugaug      660

gcccaggaua agccaacagu ggacaucgag cuggucacca cgacuguuuc aaacauggca      720

gaggugcgaa guuacugcua ugaagcuagc auaucugaua uggccuccga cucaagaugu      780

cccacacaag gagaggcgua ccuugacaag cagagcgaua cccaguaugu augcaaaagg      840

acuuuggugg accggggcug ggguaauggg uguggacugu ucggcaaggg cagucuaguc      900

acaugcgcca aauuugcaug cucuaagaaa augaccggga aguccauuca gccugaaaac      960

cucgaguacc guaucaugcu gagcgugcac ggaucgcaac acucugguau gauuguuaau     1020

gauacgggcc augaaaccga cgagaaccgc gcuaaggugg agaucacacc aaauuccccc     1080

agagccgaag cuacucuugg gggauucggc ucacuggguu uggauuguga gccuaggacc     1140

gggcucgacu uuagugaucu guauuacuua acaaugaaca auaaacacug gcugguccau     1200

aaggaauggu uccacgacau cccacucccg uggcacgccg gcgcagauac uggaacaccc     1260

cauuggaaca auaaagaggc ccuuguggaa uuuaaggacg cucacgcgaa acggcagacc     1320

guaguugugc ugggcagcca ggagggggca guccauaccg ccuuggcugg agcccuagag     1380

gcagaaaugg auggugccaa gggccgacug uccucugggc accucaaaug ccgccugaag     1440

auggacaagc ugagacugaa aggagugagc uauucauugu guacugcugc cuucacguuu     1500

acaaagauuc cugcagagac ccuccauggc acugucaccg uggaaguuca guacgcgggu     1560

acagacgggc caugcaaagu gcccgcucaa auggccguag auaugcagac ccugacuccu     1620

gucggcaggu uaauaacagc aaacccagug aucacggaga guaccgaaaa cuccaagaug     1680

augcuggagc uagauccccc guucggagac agcuacauug uuaucggcgu gggggagaaa     1740

aagauaacuc accacuggca ucggucugga ucaacaauug guaaggccuu ugaagcuacc     1800

guccgcggcg ccaaaagaau ggcagugcuc ggggacacag cuugggauuu cggaagugua     1860

ggcggugccc ugaauagccu ugggaagggc auccaccaga ucuucggagc ggccuuuaaa     1920

uccuuguucg gcgggauguc uugguuuucg caaauucuga ucggaacucu ccugaugugg     1980

cuuggccuga acaccaagaa ugguagcauu uccuugaugu gucucgcucu ugggggagug     2040

uuaauauucc ugucaacggc aguuagugcc                                      2070


<210>  1733
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1733
gccgacacca gcgugggcau cgucgggcug cuccuuacaa cugcuauggc agccgagguu       60

acgagaaggg gauccgcgua cuauauguac cuggaucgga acgacgcugg ugaagccauu      120

ucuuucccca ccacauuggg caugaauaag ugcuauauac agaucaugga uuuagggcac      180

augugugacg caacuauguc auacgagugc ccuaugcugg augaaggagu ggagccagac      240

gauguagacu guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac      300

aaaaagggug aagcccgccg aagccguaga gcugugacac uaccguccca uucuaccagg      360

aaacuccaaa cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu      420

agaguugaaa auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca      480

ugguugcucg gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc      540

gccccugcuu acucuauucg gugcaucggc gugagcaauc gcgauuuugu agagggcaug      600

uccgggggaa cuuggguuga cauuguguua gaacauggug gcugugucac cgugauggcc      660

caggauaagc caacagugga caucgagcug gucaccacga cuguuucaaa cauggcagag      720

gugcgaaguu acugcuauga agcuagcaua ucugauaugg ccuccgacuc aagauguccc      780

acacaagggg aggcguaccu ugacaagcag agcgauaccc aguauguaug caaaaggacu      840

uugguggacc ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca      900

ugcgccaaau uugcaugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc      960

gaguaccgua ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugau     1020

acgggucaug aaaccgacga gaaccgcgcu aagguggaga ucacaccaaa uucccccaga     1080

gccgaagcua cucuuggcgg guucggauca cugggcuugg auugugagcc uaggaccggu     1140

cucgacuuua gugaucugua uuacuuaaca augaacaaua aacacuggcu gguccauaag     1200

gaaugguucc acgacauccc acucccgugg cacgccgggg cagauacugg cacaccccau     1260

uggaacaaua aagaggcccu uguggaauuu aaggacgcuc acgcgaaacg gcagaccgua     1320

guugugcugg gaagccagga gggcgcaguc cauaccgccu uggcuggggc ccuagaggca     1380

gaaauggaug gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug     1440

gacaaacuua gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca     1500

aagauuccug cagagacccu ccauggaacu gucaccgugg aaguucagua cgcgggcaca     1560

gacgguccau gcaaagugcc cgcucaaaug gccguagaua ugcagacccu gacuccuguc     1620

gggagguuaa uaacagcaaa cccagugauc acggagagua ccgaaaacuc caagaugaug     1680

cuggagcuag aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag     1740

auaacucacc acuggcaucg gucuggguca acaauuggaa aggccuuuga agcuaccguc     1800

cgcggugcca aaagaauggc agugcucggc gacacagcuu gggauuucgg gaguguagga     1860

ggcgcccuga auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc     1920

uuguucggag gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu     1980

ggacugaaca ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua     2040

auauuccugu caacggcagu uagugcc                                         2067


<210>  1734
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1734
gacaccagcg ugggcaucgu cgggcugcuc cuuacaacug ccauggcugc agagguuacg       60

agaaggggau ccgccuacua uauguaccug gaucggaacg acgcggguga agcuauuucu      120

uuccccacca cauugggcau gaauaagugc uauauacaga ucauggauuu agggcacaug      180

ugugacgcca cuaugucaua cgagugcccu augcuggaug aaggagugga gccagacgau      240

guagacuguu ggugcaacac cacaaguacc uggguggucu auggcacuug ucaucacaaa      300

aagggugaag cacgccgaag ccguagagcc gugacacuac cgucccauuc uaccaggaaa      360

cuccaaacgc ggucgcagac uuggcuggag ucacgcgagu acaccaagca ccuuauuaga      420

guugaaaauu ggaucuuuag gaaccccggg uucgcucugg cagccgcugc cauugcgugg      480

uugcucggaa gcaguacauc ccagaaagug aucuaucugg ucaugauacu gcucaucgca      540

cccgccuacu cuauucggug caucggcgug agcaaucgcg auuuuguaga gggcaugucc      600

gggggaacuu ggguugacau uguguuagaa caugguggcu gugucaccgu gauggcucag      660

gauaagccaa caguggacau cgagcugguc accacgacug uuucaaacau ggccgaggug      720

cgaaguuacu gcuaugaagc aagcauaucu gauauggcuu ccgacucaag augucccaca      780

caaggggagg ccuaccuuga caagcagagc gauacccagu auguaugcaa aaggacuuug      840

guggaccggg gauggggcaa ugguuguggg cuguucggaa agggcagucu agucacaugc      900

gcgaaauuug ccugcucuaa gaaaaugacc ggcaagucca uucagccuga aaaccucgag      960

uaccguauca ugcugagcgu gcacgggucg caacacucug gaaugauugu uaaugauacg     1020

ggucaugaaa ccgacgagaa ccgcgcaaag guggagauca caccaaauuc ccccagagcu     1080

gaagccacuc uuggcggguu cggaucacug ggcuuggauu gugagccuag gaccggucuc     1140

gacuuuagug aucuguauua cuuaacaaug aacaauaaac acuggcuggu ccauaaggaa     1200

ugguuccacg acaucccacu cccguggcac gcuggggccg auacuggcac cccucauugg     1260

aacaauaaag aggcacuugu ggaauuuaag gacgcccacg cuaaacggca gacaguaguu     1320

gugcugggaa gccaggaggg cgcgguccau accgcauugg ccggggcucu agaggccgaa     1380

auggauggag caaagggucg acuguccucu ggccaccuca aaugccgccu gaagauggac     1440

aaacuuagac ugaagggggu gagcuauuca uuguguacug ccgcuuucac guuuacaaag     1500

auuccugccg agacccucca uggaacuguc accguggaag uucaguacgc aggcacagac     1560

gguccaugca aagugcccgc gcaaauggcu guagauaugc agacccugac uccugucggg     1620

agguuaauaa cagccaaccc agugaucacg gagaguaccg aaaacuccaa gaugaugcug     1680

gagcuagauc ccccguucgg cgacagcuac auuguuaucg gagugggcga gaaaaagaua     1740

acucaccacu ggcaucgguc ugggucaaca auuggaaagg cauuugaagc caccguccgc     1800

ggugcuaaaa gaauggccgu gcucggcgac acagcauggg auuucgggag uguaggaggc     1860

gcucugaaua gccuugguaa ggggauccac cagaucuucg gcgccgcguu uaaauccuug     1920

uucggaggca ugucuugguu uucgcaaauu cugaucggga cucuccugau guggcuugga     1980

cugaacacca agaauggcag cauuuccuug augugucucg cccugggugg gguguuaaua     2040

uuccugucaa cggcuguuag ugca                                            2064


<210>  1735
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  1735
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc a                                               2061


<210>  1736
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1736
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcagc cguuacgaga       60

aggggauccg cguacuauau guaccuggac cggaacgaug cuggugaggc cauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugcaacua ugucauacga augcccuaug cuggacgagg gaguggaacc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaggccc gccgaagccg uagagcugug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaauca cgcgaguaca ccaagcaccu uauuagaguu      420

gagaauugga ucuuuaggaa ccccggguuc gcacuggccg cugccgcgau ugcaugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcuuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaagg cauguccggg      600

ggaacuuggg uugauauugu guuagagcau gguggcugug ucaccgugau ggcccaggac      660

aagccaacag uggauaucga acuggucacc acgacuguuu caaacauggc agaggugcga      720

aguuacugcu augaggcuag cauaucugac auggccuccg auucaagaug ucccacacaa      780

ggggaagcgu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcc      900

aaauuugcau gcucuaagaa aaugaccggc aaguccauuc agccugagaa ccucgaauac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugagaccg augaaaaccg cgcuaaggug gagaucacac caaauucccc cagagccgag     1080

gcuacucuug gcggguucgg aucacugggc uuggacugug aaccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggagugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcagaca cuggcacacc ccauuggaac     1260

aauaaagaag cccuugugga guuuaaggau gcucacgcga aacggcagac cguaguugug     1320

cugggaagcc aggaaggcgc aguccauacc gccuuggcug gggcccuaga ggcagagaug     1380

gacggagcca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugcug ccuucacguu uacaaagauu     1500

ccugcagaaa cccuccaugg aacugucacc guggagguuc aguacgcggg cacagacggu     1560

ccaugcaaag ugcccgcuca aauggccgua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag caaacccagu gaucacggaa aguaccgaga acuccaagau gaugcuggaa     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggccu uugaggcuac cguccgcggu     1800

gccaaaagaa uggcagugcu cggcgauaca gcuugggacu ucgggagugu aggaggcgcc     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg cggccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgcuc uggguggggu guuaauauuc     2040

cugucaacgg caguuagugc c                                               2061


<210>  1737
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1737
agaggcgccg acaccagcgu ggggaucguc ggacugcucc uuacaacugc uauggcagcc       60

gcgguuacga ggcgggguuc cgcuuacuau auguaccugg aucgcaacga cgccggcgag      120

gcaauuucuu uccccaccac auuggggaug aauaagugcu auauacagau cauggauuua      180

ggacacaugu gugacgccac uaugucauac gaaugcccua ugcuggauga gggcguggaa      240

ccagacgaug uagacuguug gugcaacacc acaaguaccu ggguggucua ugguacuugu      300

caucacaaaa agggggaggc ucgacguagc agaagggcag ugacacuacc gucccauucu      360

acccggaaac uccaaacgcg cucgcagacu uggcuggaau caagagagua caccaagcac      420

cuuauuaggg uugagaauug gaucuuucgg aaccccggau ucgcccuggc ugccgcggca      480

auugccuggu ugcucggcag caguacaucc cagaaaguga ucuaucuggu caugauacug      540

cucaucgcuc cugccuacuc uauucgcugc aucggcguga gcaaucgaga uuuuguagaa      600

gggauguccg gagguacuug gguugacauu guguuagagc auggcgggug ugucaccgug      660

auggcacagg auaagccaac aguggacauc gaacugguca ccacgacugu uucaaacaug      720

gcugagguga gaaguuacug cuaugaggcc agcauaucug auauggcguc cgacucaagg      780

ugucccacac aaggagaagc cuaccuugac aagcagagcg auacccagua uguaugcaaa      840

cggacuuugg uggaccgugg cugggguaau ggguguggac uguucggcaa gggcagucua      900

gucacaugcg caaaauuugc uugcucuaag aaaaugaccg ggaaguccau ucagccugag      960

aaccucgaau accgcaucau gcugagcgug cacggaucgc aacacucugg uaugauuguu     1020

aaugauacgg gccaugagac cgacgaaaac agagccaagg uggagaucac accaaauucc     1080

cccagggcug aggccacucu ugggggauuc ggcucacugg guuuggauug ugaaccucgg     1140

accgggcucg acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc     1200

cauaaggagu gguuccacga caucccacuc ccguggcacg caggcgccga uacuggaaca     1260

ccccauugga acaauaaaga agcucuugug gaguuuaagg acgcgcacgc aaaacgacag     1320

accguaguug ugcugggcag ccaggaaggg gccguccaua ccgcuuuggc cggagcacua     1380

gaggccgaga uggauggugc uaagggccgc cuguccucug ggcaccucaa augcagacug     1440

aagauggaca agcugaggcu gaaaggagug agcuauucau uguguacugc cgcauucacg     1500

uuuacaaaga uuccugcgga aacccuccau ggcacuguca ccguggaggu ucaguacgcu     1560

gguacagacg ggccaugcaa agugcccgcc caaauggcag uagauaugca gacccugacu     1620

ccugucggcc gguuaauaac agccaaccca gugaucacgg aaaguaccga gaacuccaag     1680

augaugcugg aacuagaucc cccguucgga gacagcuaca uuguuaucgg cgugggggag     1740

aaaaagauaa cucaccacug gcaucgcucu ggaucaacaa uugguaaggc uuuugaggcc     1800

accgucagag gcgcaaaaag gauggcugug cucggggaca cagccuggga uuucggaagu     1860

guaggcggug cgcugaauag ccuugggaag ggcauccacc agaucuucgg agccgcuuuu     1920

aaauccuugu ucggcgggau gucuugguuu ucgcaaauuc ugaucggaac ucuccugaug     1980

uggcuuggcc ugaacaccaa gaaugguagc auuuccuuga ugugucucgc acuuggggga     2040

guguuaauau uccugucaac ggccguuagu gcu                                  2073


<210>  1738
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1738
agaggccuga gcuccaggaa gcggcgcucu cacgacgugc ucaccgucca guuccuuauc       60

cuggggaugu uguuaaugac aggagguguu acucgacgug gcucagccua cuauauguac      120

cuggauagaa acgacgcugg ggaggcaauu aguuuuccca cgacccuagg aaugaauaaa      180

ugcuauauac aaaucaugga ucucggccau augugugacg ccacaaugag cuacgaaugc      240

ccuaugcugg augagggugu ggaaccagac gauguagacu guuggugcaa cacuaccucc      300

acaugggugg ucuaugggac cugucaccau aagaaaggag aggcgaggcg gucucgcaga      360

gcugugacuc uuccgucgca cucaacaagg aagcugcaga cccggagcca gacgugguug      420

gaaagucgcg aguacacuaa acaucucauu cgaguugaga auuggaucuu cagaaacccc      480

ggcuuugccc uggcagccgc ugcaauugcc uggcugcucg gcuccucuac cagccagaag      540

gugaucuauu uagucaugau acugcuuauc gcuccugccu acuccauuag gugcaucggg      600

gugucaaauc gggauuucgu agaaggaaug agugguggca cauggguuga cauuguguug      660

gagcacgggg gaugugucac ugugauggcg caagauaagc caaccgugga caucgaacug      720

gucacaacca cgguuagcaa cauggcagag gugcguucuu acugcuauga ggccuccaua      780

ucagauaugg cuagcgacag ucgcuguccc acucagggcg aagccuaccu agacaaacag      840

ucugauacac aguauguaug caagagaacc cucguggaca gggguugggg gaauggaugu      900

ggccuguuug gcaaaggguc ccuugucacu ugcgcaaagu ucgcuugcag caaaaagaug      960

acaggaaagu cgauucaacc ugagaaccug gaauaccgga ucauguuguc ugugcacggu     1020

ucccagcauu caggcaugau uguuaaugau accgggcacg agacggacga aaaccgagcc     1080

aaaguggaga ucaccccaaa uaguccccgc gcggaggcca cacucggagg cuuugguagc     1140

cugggguuag auugugaacc uagaacuggc cuggacuucu ccgaucucua uuaccuuacc     1200

augaacaaua agcauuggcu gguccacaaa gagugguuuc acgacauccc auugccgugg     1260

caugcaggag cugauacagg cacuccccac uggaacaaua aggaagcccu aguggaguuc     1320

aaagacgcuc augccaagag gcagaccgua guugugcugg ggucucagga aggagcaguc     1380

cacacagccc ucgcuggugc gcuggaggca gagauggaug gcgccaaagg gcggcuuagc     1440

ucaggacauc ugaagugccg cuugaagaug gacaaacuca gacugaaggg cgugaguuau     1500

uccuuaugua ccgcugccuu uacuuucacg aaaauuccug cagaaacacu gcacgguacc     1560

gucacugugg agguucaaua cgccgggacc gacggcccau gcaaggugcc cgcucagaug     1620

gccguagaua ugcagacacu aaccccuguc ggaaggcuca uaacugcaaa cccagugauc     1680

acagaaagca cggagaacuc uaaaaugaug cuggaacuug aucccccguu uggcgacuca     1740

uacauuguua ucgggguggg agagaagaag auaacccacc auuggcaccg uagugguagc     1800

acuauuggca aagcguucga ggcuacaguc cgaggggcca agcggauggc aguguuggga     1860

gacaccgccu gggauuucgg cuccguaggu ggggcucuca auucucuggg caaaggaauc     1920

caucaaaucu uuggcgccgc auucaagucg cuguuugggg gaaugagcug guucucccag     1980

auucuuaucg gcacacuguu gauguggcuc ggucugaaca cuaaaaaugg gucaauuagu     2040

uuaauguguc uggcucuagg aggcgugcuc auauuucugu cuaccgccgu uagcgcg        2097


<210>  1739
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1739
agaggccuga gcuccaggaa gcggcgcucu cacgacgugc ucaccgucca guuccuuauc       60

cuggggaugu uguuaaugac aggagccguu acucgacgug guucagcuua cuauauguac      120

cuggauagaa acgacgcagg cgaggccauu aguuuuccca cgacccuagg gaugaauaaa      180

ugcuauauac aaaucaugga ucucggacau augugugacg cgacaaugag cuacgaaugc      240

ccuaugcugg augagggcgu ggaaccagac gauguagacu guuggugcaa cacuaccucc      300

acaugggugg ucuaugguac cugucaccau aagaaagggg aggcuaggcg gucucgcaga      360

gccgugacuc uuccgucgca cucaacaagg aagcugcaga cccggagcca gacgugguug      420

gaaagucgcg aguacacuaa acaucucauu cgaguugaga auuggaucuu cagaaacccc      480

ggauuugcac uggccgcugc agccauugcu uggcugcucg gcuccucuac cagccagaag      540

gugaucuauu uagucaugau acugcuuauc gccccugcgu acuccauuag gugcaucggc      600

gugucaaauc gggauuucgu agaagggaug aguggaggua cauggguuga cauuguguug      660

gagcacggcg ggugugucac ugugauggca caagauaagc caaccgugga caucgaacug      720

gucacaacca cgguuagcaa cauggccgag gugcguucuu acugcuauga ggcuuccaua      780

ucagauaugg ccagcgacag ucgcuguccc acucagggag aagcauaccu agacaaacag      840

ucugauacac aguauguaug caagagaacc cucguggaca ggggcugggg uaaugggugu      900

ggacuguuug gcaaaggcuc ccuugucacu ugcgcuaagu ucgccugcag caaaaagaug      960

acagggaagu cgauucaacc ugagaaccug gaauaccgga ucauguuguc ugugcacgga     1020

ucccagcauu cagguaugau uguuaaugau accggccacg agacggacga aaaccgagcg     1080

aaaguggaga ucaccccaaa uaguccccgc gccgaggcaa cacucggggg auuuggcagc     1140

cuggguuuag auugugaacc uagaacuggg cuggacuucu ccgaucucua uuaccuuacc     1200

augaacaaua agcauuggcu gguccacaaa gagugguuuc acgacauccc auugccgugg     1260

caugcuggcg ccgauacagg aacuccccac uggaacaaua aggaagcucu aguggaguuc     1320

aaagacgccc augcaaagag gcagaccgua guugugcugg gcucucagga aggggccguc     1380

cacacagcuc ucgcgggagc acuggaggcc gagauggaug gugcuaaagg ccggcuuagc     1440

ucagggcauc ugaagugccg cuugaagaug gacaaacuca gacugaaggg agugaguuau     1500

uccuuaugua ccgccgcauu uacuuucacg aaaauuccug ccgaaacacu gcacggcacc     1560

gucacugugg agguucaaua cgcugguacc gacgggccau gcaaggugcc cgcccagaug     1620

gcaguagaua ugcagacacu aaccccuguc ggcaggcuca uaacugcgaa cccagugauc     1680

acagaaagca cggagaacuc uaaaaugaug cuggaacuug aucccccguu uggagacuca     1740

uacauuguua ucggcguggg ggagaagaag auaacccacc auuggcaccg uaguggaagc     1800

acuauuggua aggcuuucga ggccacaguc cggggcgcaa aacgaauggc cguguugggg     1860

gacaccgcuu gggauuucgg auccguaggc ggugcccuca auucucuggg gaaaggcauc     1920

caucaaaucu uuggagcagc uuucaagucg cuguuuggcg ggaugagcug guucucccag     1980

auucuuaucg gaacacuguu gauguggcuc ggccugaaca cuaaaaaugg uucaauuagu     2040

uuaauguguc uggcccuagg gggagugcuc auauuucugu cuaccgcggu uagcgcc        2097


<210>  1740
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long CDS opt

<400>  1740
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

auaguguuag agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac      720

auugagcugg ucaccacgac uguuucaaau auggcugaag ugcguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aaguggaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guggaguuua aagacgcgca cgcaaagagg cagaccguag uugugcuggg cagccaggag     1380

ggggccgucc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagacccug acuccugucg gcagguuaau uacagccaac     1680

ccagugauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggagugcuga uuuuccuguc aacggccguu     2100

agugcugacg ucggcugcuc uguggauuuu agcaaa                               2136


<210>  1741
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long CDS opt

<400>  1741
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca cuugugacgc caccauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

acaaccagua cuuggguggu cuaugguaca ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacccu accgucccau ucuacgagga agcuccaaac ucggucgcag      420

accuggcugg agucacgcga auacacaaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguacu      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac cuggguugac      660

guggucuuag agcauggcgg gugugugaca gugauggcac aggauaagcc aaccgucgac      720

auagagcugg uuacgacuac agugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cccaaggaga agccuaccuu      840

gacaaacaga gcgauacuca guaugugugc aagagaacau uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugaccu gcgcaaaguu ugcuugcucu      960

aagaaaauga cggggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua ccggccauga gacagacgag     1080

aaucgagcca aagucgaaau cacuccaaac ucaccccgcg cugaggccac ccuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaacagggc ucgacuuuag ugaucuguau     1200

uacuuaacua ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauaccgga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auacugcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ggccgcauuc acauuuacca aaauaccugc ggaaacucuc     1560

cauggcaccg ugacaguuga ggugcaguac gcugguaccg acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacucug acaccugugg gcagguuaau cacggccaac     1680

ccaguuauua ccgaaaguac ugaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacacacca cuggcaucgu     1800

ucuggaucaa ccaucgguaa ggcuuuugag gccacagugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacugccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacccuccug auguggcuug gccugaaugc aaagaacggu     2040

agcauauccu ugaugugucu cgccuuaggg ggaguucuga ucuuccuguc aacggcuguc     2100

agugccgacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1742
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long CDS opt

<400>  1742
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacauugggg      180

gugaauaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgucacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauaa aaguggaaaa uuggaucuuu      480

agaaacccgg gauucgcucu gguugccgug gcgauugcau gguugcucgg caguuccaca      540

ucucagaagg ucaucuaccu ggugaugauu cugcucaucg cccccgcuua uagcauaagg      600

ugcauuggcg uauccaaucg ggauuuuguu gaggggaugu caggagguac uuggguggac      660

gucguguuag aacacggcgg gugugugacc gucauggccc aggauaaacc uacaguugac      720

aucgagcugg ugaccacgac uguaaguaac auggcagagg ugcguagcua cugcuacgag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgucugc aaaagaacuu ugguggacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuaguuacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauccagccc gaaaaccucg aguaucggau caugcugucg     1020

gugcacggau cucaacauuc cgguaugauu ggcuacgaaa ccgaugagga ccgagcaaag     1080

gucgagguga caccuaauuc accacgcgcu gaagccacuc uugggggauu cggcagucug     1140

gguuuggauu gugagcccag aaccgggcuc gacuuuagcg aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu acauaaggaa ugguuccacg acauaccucu cccauggcac     1260

gcuggcgccg auacuggaac cccgcauugg aacaauaaag aggcacuugu ugaauuuaag     1320

gacgcccacg cuaagaggca gacagugguc gugcugggcu cccaggaggg ggcgguccau     1380

accgcauugg ccggagcucu agaggccgaa auggauggug caaaaggccg gcuguucucu     1440

gggcaccuca agugccgccu gaaaauggac aaacuuagac ugaagggagu gagcuauuca     1500

uuguguacug ccgcuuuuac guucacaaag guucccgccg agacccucca uggcacugug     1560

accguagaag uccaguacgc agguacagac gggccuugca agaucccagu gcaaauggcg     1620

guugauaugc agacccugac ucccgugggc agguuaauua cagcuaaccc ugucaucacg     1680

gagaguaccg aaaauuccaa aaugaugcug gagcuagacc cacccuuugg agauagcuac     1740

auagugauug gcguagggga caagaaaauc acucaccacu ggcaucguuc uggaucaaca     1800

aucgguaagg ccuucgaggc aaccgugcgg ggcgccaaac gaauggcugu ucucggggau     1860

acagccuggg acuucggaag ugucggcggu guguuuaaca gccuggggaa gggcauucac     1920

cagaucuucg gagcagcuuu uaagucccuu uucggcggga ugucuugguu uucgcaaauu     1980

uugauaggaa cucugcucgu guggcugggc cuuaacacca aaaaugguag caucucccug     2040

acguguuugg cccucggggg agucaugauu uuccugucaa ccgcgguuag ugccgaugug     2100

ggcugcucug uggacuuuag caag                                            2124


<210>  1743
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long CDS opt

<400>  1743
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1744
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long CDS opt

<400>  1744
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacauugggg      180

gugaauaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgucacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauaa aaguggaaaa uuggaucuuu      480

agaaacccgg gauucgcucu gguugccgug gcgauugcau gguugcucgg caguuccaca      540

ucucagaagg ucaucuaccu ggugaugauu cugcucaucg cccccgcuua uagcauaagg      600

ugcauuggcg uauccaaucg ggauuuuguu gaggggaugu caggagguac uuggguggac      660

gucguguuag aacacggcgg gugugugacc gucauggccc aggauaaacc uacaguugac      720

aucgagcugg ugaccacgac uguaaguaac auggcagagg ugcguagcua cugcuacgag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgucugc aaaagaacuu ugguggacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuaguuacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauccagccc gaaaaccucg aguaucggau caugcugucg     1020

gugcacggau cucaacauuc cgguaugauu gucaaugaua ccggcuacga aacagacgag     1080

aaccgagcaa agguggaggu aacuccuaau ucaccacgcg cugaagccac ccuuggggga     1140

uucggcaguc uggguuugga uugugagccc agaacagggc ucgacuuuag cgaucuguau     1200

uacuuaacua ugaacaauaa acacuggcug guucauaagg aaugguucca cgacauaccu     1260

cucccauggc acgcuggcgc cgauaccgga acaccgcauu ggaacaauaa agaggcacuu     1320

guggaauuua aggacgccca cgcuaagagg cagaccgucg ugguccuggg cucccaggag     1380

ggggcggugc auacugcauu ggccggagcu cuagaggccg aaauggaugg ugcaaaaggc     1440

aagcuguucu cugggcaccu caaaugccgg cugaagaugg acaaacuucg ccugaaggga     1500

guuagcuauu cauuguguac ggccgcuuuu acauucacca aggugcccgc cgagacucuc     1560

cauggcaccg uaacagucga agugcaguac gcagguaccg acgggccuug caaaauccca     1620

guucaaaugg cgguggauau gcagacucug acacccgucg gcagauuaau uacggcuaac     1680

ccugugauca ccgagaguac ugaaaacucc aagaugaugc uggagcuaga cccacccuuu     1740

ggagauagcu acauaguaau uggcgugggg gacaaaaaga ucacacacca cuggcauagg     1800

ucuggaucaa ccaucgguaa agccuucgag gcaacaguuc guggcgccaa gcggauggcu     1860

guccucgggg auacugccug ggacuucgga agugugggcg guguguuuaa uagccugggg     1920

aagggcauuc accagaucuu cggagcagcu uuuaaauccc uuuucggcgg gaugucuugg     1980

uuuucgcaaa uuuugauagg aacccugcuc gucuggcugg gccuuaacac gaagaauggu     2040

agcaucuccc ugaccuguuu ggcccucggg ggaguuauga uuuuccuguc aacagcggug     2100

agugccgaug ugggcugcuc uguagacuuu agcaaa                               2136


<210>  1745
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long CDS opt

<400>  1745
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

gugggacugc uccuuacaac ugcaauggcc gcggaaauua cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaaucu cauucgccac cacauugggg      180

gucaauaaau gccacguuca gauuauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcgug gagccugacg auguagacug uuggugcaac      300

accacaagca ccuggguggu cuacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgugacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauca aaguugaaaa uuggauauuu      480

agaaacccgg gauucacacu gguggcuguc gccaucgcgu gguugcucgg caguuccacu      540

ucucagaagg ugauuuaccu gguaaugauc cugcucauug cacccgccua uagcaucagg      600

ugcauaggcg uuuccaaucg ggauuuugug gaggggaugu caggagguac cugggucgac      660

gugguguuag aacacggcgg gugugucaca guuauggcuc aggauaaacc uaccguggac      720

auugagcugg uaacgacuac agugaguaac auggccgagg uccguagcua cugcuacgaa      780

gcaucuaucu ccgauauggc uucagacagc cgcuguccaa cccaaggaga ggccuaucuu      840

gacaagcaga gugauacuca guacgugugc aaaagaacau ugguugacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuagugaccu gcgcgaaguu uacgugcucc      960

aaaaagauga ccgggaaaag cauucagccc gaaaaccucg aguaucggau caugcugucg     1020

guccacggau cucaacauuc cgguaugauc gugaaugaug aaaaccgagc caagguagag     1080

guuacaccua auucaccacg cgcagaggcu acucuuggcg gguucggaag ucugggcuug     1140

gacugugaac ccagaaccgg ucucgauuuu agcgaccugu acuauuuaac aaugaacaau     1200

aaacacuggc uggugcauaa ggagugguuc cacgauauuc cucucccaug gcacgccggg     1260

gcugacacug gcaccccgca uuggaacaau aaagaagccc uugucgaguu uaaggaugca     1320

cacgccaaga ggcagacagu ggucgugcug ggaucccagg aaggcgcugu ucauaccgcg     1380

uuggcagggg cccuagaggc ugagauggac ggagccaaag gucggcuguu cucuggccac     1440

cucaagugcc gccugaaaau ggauaaacuu agacugaagg gggugagcua cucauugugu     1500

acugcagccu uuacguucac aaagguaccc gcugaaaccc uccauggaac ugucaccgug     1560

gagguucagu augccggcac agacgguccu ugcaaggugc cagcacaaau ggcggucgac     1620

augcagaccc ugacucccgu ggggagguua auaacagcua acccuguaau cacggaaagu     1680

accgagaacu ccaaaaugau gcuggaacua gauccacccu uuggcgacag cuacauugug     1740

aucggaguug gcgauaagaa aauaacucac cacuggcauc guucuggguc aacaauugga     1800

aaggccuucg aggcaaccgu ccggggugcc aaacgaaugg cugugcucgg cgacacagcc     1860

ugggauuucg ggaguguggg aggcgucuuu aauagccugg guaaggggau ccaccagauc     1920

uucggcgcag cuuuuaaguc ccuuuucgga ggcaugucuu gguuuucgca aauuuugauc     1980

gggacucugc ucguuuggcu gggacuuaac accaaaaaug gcagcauuuc ccugacgugu     2040

uuggcccucg guggggugau gauauuccug ucaaccgcgg ugagugccga cguaggaugc     2100

ucugucgauu uuagcaag                                                   2118


<210>  1746
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long CDS opt

<400>  1746
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacauugggg      180

gugaauaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccgga agaaagggga agcacgcaga      360

uccaggcggg ccgucacacu accaucucau ucgacccgca agcuccaaac gcgaucacag      420

acuuggcugg agagcagaga guauaccaag caccuuauaa aaguggaaaa uuggaucuuu      480

aggaacccgg gauucgcucu gguugccgug gcgauugcau gguugcucgg caguuccaca      540

ucucagaagg ucaucuaccu ggugaugauu cugcucaucg cccccgcuua uagcauacgg      600

ugcauuggcg uauccaaucg ugauuuuguu gaggggaugu caggagguac uuggguggac      660

gucguguuag aacauggcgg gugugugacc gucauggccc aggauaaacc uacaguugac      720

aucgagcugg ugaccacgac uguaaguaac auggcagagg ugcgcagcua cugcuacgag      780

gcuucuauuu ccgauauggc cucagacagc agauguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgucugc aaaaggacuu ugguggaccg gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuaguuacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauccagccc gaaaaccucg aguaucgaau caugcugucg     1020

gugcacggau cucaacacuc cgguaugauu gucaaugaua ccggcuacga aacagacgag     1080

aaccgcgcaa agguggaggu aacuccuaau ucaccaagag cugaagccac ccuuggggga     1140

uucggcaguc uggguuugga uugugagccc aggacagggc ucgacuuuag cgaucuguau     1200

uacuuaacua ugaacaauaa acauuggcug guucacaagg aaugguucca ugacauaccu     1260

cucccauggc acgcuggcgc cgauaccgga acaccgcacu ggaacaauaa agaggcacuu     1320

guggaauuua aggacgccca ugcuaagcgg cagaccgucg ugguccuggg cucccaggag     1380

ggggcggugc acacugcauu ggccggagcu cuagaggccg aaauggaugg ugcaaaaggc     1440

aagcuguucu cugggcaucu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

guuagcuauu cauuguguac ggccgcuuuu acauucacca aggugcccgc cgagacucuc     1560

cacggcaccg uaacagucga agugcaguac gcagguaccg acgggccuug caaaauccca     1620

guucaaaugg cgguggauau gcagacucug acacccgucg gcagguuaau uacggcuaac     1680

ccugugauca ccgagaguac ugaaaacucc aagaugaugc uggagcuaga cccacccuuu     1740

ggagauagcu acauaguaau uggcgugggg gacaaaaaga ucacacauca cuggcaccgu     1800

ucuggaucaa ccaucgguaa agccuucgag gcaacaguuc ggggcgccaa gcgaauggcu     1860

guccucgggg auacugccug ggacuucgga agugugggcg guguguuuaa uagccugggg     1920

aagggcauuc aucagaucuu cggagcagcu uuuaaauccc uuuucggcgg gaugucuugg     1980

uuuucgcaaa uuuugauagg aacccugcuc gucuggcugg gccuuaacac gaagaauggu     2040

agcaucuccc ugaccuguuu ggcccucggg ggaguuauga uuuuccuguc aacagcggug     2100

agugccgaug ugggcugcuc uguagacuuu agcaaa                               2136


<210>  1747
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long CDS opt

<400>  1747
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacauugggg      180

gugaauaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgucacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauaa aaguggaaaa uuggaucuuu      480

agaaacccgg gauucgcucu gguugccgug gcgauugcau gguugcucgg caguuccaca      540

ucucagaagg ucaucuaccu ggugaugauu cugcucaucg cccccgcuua uagcauaagg      600

ugcauuggcg uauccaaucg ggauuuuguu gaggggaugu caggagguac uuggguggac      660

gucguguuag aacacggcgg gugugugacc gucauggccc aggauaaacc uacaguugac      720

aucgagcugg ugaccacgac uguaaguaac auggcagagg ugcguagcua cugcuacgag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgucugc aaaagaacuu ugguggacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuaguuacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauccagccc gaaaaccucg aguaucggau caugcugucg     1020

gugcacggau cucaacauuc cgguaugacc gucaaugaua uuggcuacga aacagacgag     1080

aaccgagcaa agguggaggu aacuccuaau ucaccacgcg cugaagccac ccuuggggga     1140

uucggcaguc uggguuugga uugugagccc agaacagggc ucgacuuuag cgaucuguau     1200

uacuuaacua ugaacaauaa acacuggcug guucauaagg aaugguucca cgacauaccu     1260

cucccauggc acgcuggcgc cgauaccgga acaccgcauu ggaacaauaa agaggcacuu     1320

guggaauuua aggacgccca cgcuaagagg cagaccgucg ugguccuggg cucccaggag     1380

ggggcggugc auacugcauu ggccggagcu cuagaggccg aaauggaugg ugcaaaaggc     1440

aagcuguucu cugggcaccu caaaugccgg cugaagaugg acaaacuucg ccugaaggga     1500

guuagcuauu cauuguguac ggccgcuuuu acauucacca aggugcccgc cgagacucuc     1560

cauggcaccg uaacagucga agugcaguac gcagguaccg acgggccuug caaaauccca     1620

guucaaaugg cgguggauau gcagacucug acacccgucg gcagauuaau uacggcuaac     1680

ccugugauca ccgagaguac ugaaaacucc aagaugaugc uggagcuaga cccacccuuu     1740

ggagauagcu acauaguaau uggcgugggg gacaaaaaga ucacacacca cuggcauagg     1800

ucuggaucaa ccaucgguaa agccuucgag gcaacaguuc guggcgccaa gcggauggcu     1860

guccucgggg auacugccug ggacuucgga agugugggcg guguguuuaa uagccugggg     1920

aagggcauuc accagaucuu cggagcagcu uuuaaauccc uuuucggcgg gaugucuugg     1980

uuuucgcaaa uuuugauagg aacccugcuc gucuggcugg gccuuaacac gaagaauggu     2040

agcaucuccc ugaccuguuu ggcccucggg ggaguuauga uuuuccuguc aacagcggug     2100

agugccgaug ugggcugcuc uguagacuuu agcaaa                               2136


<210>  1748
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long CDS opt

<400>  1748
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacauugggg      180

gugaauaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgucacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauaa aaguggaaaa uuggaucuuu      480

agaaacccgg gauucgcucu gguugccgug gcgauugcau gguugcucgg caguuccaca      540

ucucagaagg ucaucuaccu ggugaugauu cugcucaucg cccccgcuua uagcauaagg      600

ugcauuggcg uauccaaucg ggauuuuguu gaggggaugu caggagguac uuggguggac      660

gucguguuag aacacggcgg gugugugacc gucauggccc aggauaaacc uacaguugac      720

aucgagcugg ugaccacgac uguaaguaac auggcagagg ugcguagcua cugcuacgag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgucugc aaaagaacuu ugguggacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuaguuacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauccagccc gaaaaccucg aguaucggau caugcugucg     1020

gugcacggau cucaacauuc cgguaugauu ggcuacgaaa ccgaugagaa ucgagcaaag     1080

gucgagguga caccuaacuc accacgcgcu gaagccacuc uugggggauu cggcagucug     1140

gguuuggacu gugagcccag aaccgggcuc gauuuuagcg accuguauua cuuaacaaug     1200

aauaacaaac acuggcuggu acauaaggaa ugguuccacg auauaccucu cccauggcac     1260

gcuggcgccg acacuggaac cccgcauugg aauaacaaag aggcacuugu ugaauuuaag     1320

gaugcccacg cuaagaggca gacagugguc gugcugggcu cccaggaggg ggcgguccau     1380

accgcauugg ccggagcucu agaggccgaa auggacggug caaaaggcaa gcuguucucu     1440

gggcaccuca aaugccggcu gaagauggau aaacuucgcc ugaagggagu gagcuauuca     1500

uuguguacug ccgcuuuuac guucacaaag guucccgccg agacccucca uggcacugug     1560

accguagaag uccaguacgc agguacagac gggccuugca aaaucccagu gcaaauggcg     1620

guugacaugc agacccugac ucccgugggc agauuaauua cagcuaaucc ugucaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc cacccuuugg agacagcuac     1740

auagugauug gcguagggga uaaaaagauc acucaccacu ggcauagguc uggaucaaca     1800

aucgguaaag ccuucgaggc aaccgugcgu ggcgccaagc ggauggcugu ucucggggac     1860

acagccuggg auuucggaag ugucggcggu guguuuaaca gccuggggaa gggcauucac     1920

cagaucuucg gagcagcuuu uaaaucccuu uucggcggga ugucuugguu uucgcaaauu     1980

uugauaggaa cucugcucgu guggcugggc cuuaauacca agaacgguag caucucccug     2040

acguguuugg cccucggggg agucaugauu uuccugucaa ccgcgguuag ugccgacgug     2100

ggcugcucug uggauuuuag caaa                                            2124


<210>  1749
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long CDS opt

<400>  1749
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

agaggcaucc accagauuuu cggagccgcu uuuaaauccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaag                               2136


<210>  1750
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long CDS opt

<400>  1750
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacgcaag cguggggaua       60

gucggacugc uccuuaccac agccauggcg gcugaaguua cucgacgugg uuccgccuac      120

uauauguacc uggauagaaa ugacgcaggc gaggccaucu cuuuccccac gaccuugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc uacaauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

acuaccagua cauggguggu cuaugguacc ugucaucaca agaaagggga ggcaaggcgg      360

agccgcagag ccgugacucu accgucccau ucuacaagga agcuccaaac ccggucgcag      420

acguggcugg agucacgcga auacacuaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcucu ggccgcggca gccauagcuu gguugcucgg cagcaguacc      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg ccccugcaua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac auggguugac      660

guggucuuag agcauggcgg gugugugacu gugauggcuc aggauaagcc aaccgucgac      720

auagagcugg uuacaaccac ggugucaaau auggccgaag uacguaguua cugcuaugag      780

gcgagcauuu cugauauggc cuccgacuca cgcuguccca cucaaggaga agcauaccuu      840

gacaaacaga gcgauacaca guaugugugc aagagaaccu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacuu gcgcuaaguu ugccugcucu      960

aagaaaauga cagggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua ccggccauga gacggacgag     1080

aaucgagcua aagucgaaau caccccaaac ucaccccgcg ccgaggcaac acuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaacugggc ucgacuuuag ugaucuguau     1200

uacuuaacca ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc ugauacagga acuccccauu ggaauaacaa ggaagcgcuu     1320

guagaguuua aagacgcaca cgccaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggcugugc auacagccuu ggcaggagcc cuagaagcug agauggaugg ugccaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac cgcagcguuc acuuuuacga aaauaccugc ugaaacacuc     1560

cauggcaccg ugacuguuga ggugcaguac gccgguaccg acgggccaug caagguaccc     1620

gcacaaaugg ccgucgauau gcagacacug accccugugg gcagguuaau cacugcuaac     1680

ccaguuauua cagaaaguac ggaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacccacca cuggcaucgu     1800

ucuggaucaa cuaucgguaa agccuuugag gcaacagugc ggggcgcuaa gcgaauggcc     1860

guacucgggg acaccgcgug ggauuucgga agugugggcg gugcccugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagcugca uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacacuccug auguggcuug gccugaauac uaagaacggu     2040

agcauauccu ugaugugucu cgccuuaggg ggaguucuga ucuuccuguc aaccgcuguc     2100

agugccgacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1751
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long CDS opt

<400>  1751
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gacuggccuu ggcugccgcg gcaauagccu ggcuccuggg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cucuuaaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuucgua gaagggaugu ccggagguac uuggguugac      660

gugguccugg agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcuug uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuacuug      840

gacaaacaga gcgauaccca guaugugugc aagagaacuc uggucgacag gggcuggggu      900

aacgggugug gacuauuugg caaaggcagu cucgugacau gcgcaaaguu cgcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucugg aauaccggau uaugcuuagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucugggaggg     1140

uuuggcuccu ugggucucga uugugaaccu agaaccgggc uggacuucag ugauuuauau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguuuca cgacauucca     1260

cuuccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucug     1320

guagaguuca aagacgcgca cgcaaagagg cagaccguug uggucuuggg cagccaggag     1380

ggggccgugc auaccgcucu agccggagca cuggaagccg agauggaugg ugcuaagggc     1440

cggcucuccu cugggcaccu gaaaugccgc cuuaagaugg acaaacugag auugaaggga     1500

gucagcuauu cacucuguac ugccgcauuu acguucacaa aaauaccugc ggaaacccug     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagaccuua acuccugugg gcaggcugau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aagaugaugc uagagcucga ucccccguuu     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuucgag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacuggggg acacagccug ggauuuugga agugugggcg gugcgcuuaa cagcuugggg     1920

aaaggcaucc accagauuuu cggagccgcu uucaaguccc uguuuggcgg gaugucuugg     1980

uucucgcaaa uccucauugg aacucugcuu auguggcugg gcuugaauac caagaacggu     2040

agcauauccc ucaugugucu ggcauuaggg ggaguucuga ucuuucuauc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuc agcaaa                               2136


<210>  1752
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long CDS opt

<400>  1752
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cugguaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc aucagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1753
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long CDS opt

<400>  1753
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaaa uucguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aaguggaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guggaguuua aagacgcgca cgcaaagagg cagaccguag uugugcuggg cagccaggag     1380

ggggccgucc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagacccug acuccugucg gcagguuaau uacagccaac     1680

ccagugauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggagugcuga uuuuccuguc aacggccguu     2100

agugcugacg ucggcugcuc uguggauuuu agcaaa                               2136


<210>  1754
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long CDS opt

<400>  1754
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguacug     1620

gcccaaaugg cagucgauau gcagaccuua acucccgugg gcaggcugau cacagccaac     1680

ccuguuauua cggaaaguac cgaaaauucc aagaugaugc uagagcucga cccacccuuc     1740

ggagauagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacuggggg acacagccug ggauuucgga agugugggcg gugcgcuuaa cagcuugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuucgcaaa uccucauugg aacucugcuu auguggcugg gcuugaauac caagaacggu     2040

agcauauccc ucaugugucu ggcauuaggg ggaguucuga ucuuccuauc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1755
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long CDS opt

<400>  1755
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuacccugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

acaaccagua cuuggguggu cuaugguaca ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacccu accgucccau ucuacgagga agcuccaaac ucggucgcag      420

accuggcugg agucacgcga auacacaaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguacu      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac cuggguugac      660

guggucuuag agcauggcgg gugugugaca gugauggcac aggauaagcc aaccgucgac      720

auagagcugg uuacgacuac agugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cccaaggaga agccuaccuu      840

gacaaacaga gcgauacuca guaugugugc aagagaacau uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugaccu gcgcaaaguu ugcuugcucu      960

aagaaaauga cggggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua ccggccauga gacagacgag     1080

aaucgagcca aagucgaaau cacuccaaac ucaccccgcg cugaggccac ccuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaacagggc ucgacuuuag ugaucuguau     1200

uacuuaacua ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauaccgga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auacugcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ggccgcauuc acauuuacca aaauaccugc ggaaacucuc     1560

cauggcaccg ugacaguuga ggugcaguac gcugguaccg acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacucug acaccugugg gcagguuaau cacggccaac     1680

ccaguuauua ccgaaaguac ugaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacacacca cuggcaucgu     1800

ucuggaucaa ccaucgguaa ggcuuuugag gccacagugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacugccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacccuccug auguggcuug gccugaauac gaagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aaccgccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1756
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long CDS opt

<400>  1756
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aacggucgug     2100

agugccgacg ugggcugcuc ugucgauuuu agcaaa                               2136


<210>  1757
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long CDS opt

<400>  1757
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagug cuuggguggu cuaugguacc ugucaucaca agaaagggga ggcaaggcgg      360

agccgcagag ccgugacucu accgucccau ucuacaagga agcuccaaac ccggccccag      420

acguggcugg agucgcgcga auacacuaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaauccug gauucgcucu ggccgcggca gccauagcuu gguugcucgg cucaagcacc      540

agucagaaag ugaucuaucu ggucaugauu cugcucaucg ccccagcaua cuccauuagg      600

ugcaucggcg ugucuaaccg ggauuuugua gaagggauga gcggagguac auggguugac      660

guggucuuag agcauggcgg gugugugacu gugauggcuc aggauaagcc caccgucgac      720

auagagcugg uuacaaccac gguguccaau auggccgaag uacguucaua cugcuaugag      780

gcgaguauua gcgauauggc cucugacucc cgcuguccua cucaaggaga agcauaccuu      840

gacaaacagu cagauacaca guaugugugc aagagaaccu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagc cuagugacuu gcgcuaaguu ugccugcagu      960

aagaaaauga cagggaaguc uauccagcca gagaaucucg aauaccggau uaugcugucc     1020

guucacggaa gccaacacuc ggguaugauc gugaacgaua ccggccauga gacggacgag     1080

aaucgagcua aagucgaaau cacccccaac ucuccucgcg ccgaggcaac acuuggggga     1140

uucggcuccc uggguuugga uugugaacca agaacugggc ucgacuuuuc agaucuguau     1200

uacuuaacca ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauuccg     1260

cuccccuggc acgccggcgc ugauacagga acuccucauu ggaauaacaa ggaagcgcuu     1320

guagaguuua aagacgcaca cgccaagagg cagaccguug ugguccuggg cagucaggag     1380

ggggcugugc auacagccuu ggcaggagcc cuagaagcug agauggaugg ugccaagggc     1440

cggcugagcu ccgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucuuaua gcuuguguac cgcagcguuc acuuuuacga aaauaccagc ugaaacacuc     1560

cauggcaccg ugacuguuga ggugcaguac gccgguaccg acgggcccug caagguaccu     1620

gcacaaaugg ccgucgauau gcagacacug accccagugg gcagguuaau cacugcuaac     1680

cccguuauua cagaaucaac ggagaauagu aagaugaugc uggagcuaga cccgccuuuc     1740

ggagauuccu acaucgugau aggcgucggg gagaaaaaga uuacccacca cuggcaucgu     1800

agcggaucua cuaucgguaa agccuuugaa gcaacagugc ggggcgcuaa gcgaauggcc     1860

guacucgggg acaccgcgug ggauuucgga ucagugggcg gugcccugaa cagucuuggg     1920

aaaggcaucc accagauuuu cggagcugca uuuaagagcu uguucggcgg gauguccugg     1980

uuuucucaaa uccugauugg aacacuccug auguggcuug gccugaauac uaagaacggu     2040

ucgaucagcu ugaugugucu cgccuuaggg ggaguucuga uauuccuguc caccgcuguc     2100

ucagccgacg ugggcugcag uguggauuuu ucuaaa                               2136


<210>  1758
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long CDS opt

<400>  1758
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

uccaaaaagg ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaaacc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacagggaga agccuaccuu      840

gacaagcaaa gcgauaccca guaugugugc aaaagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caagggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aaaaagauga ccgggaaauc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcagcacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aggucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa acacuggcug gugcauaagg agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa agaagcucuu     1320

guagaguuua aggacgcgca cgcaaagagg caaaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaaaggc     1440

cggcuguccu cugggcaccu caagugccgc cugaaaaugg acaagcugag acugaaagga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa agauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccagaugg cagucgauau gcaaacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aaaaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaagaaaa uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aagggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaga uccugauugg aacucuccug auguggcuug gccugaauac caaaaacggu     2040

agcauauccu ugaugugucu cgcacuuggg ggaguuuuaa ucuuccuguc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaag                               2136


<210>  1759
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long CDS opt

<400>  1759
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

aggcagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cuccauucgg      600

ugcaucggcg ugucuaaccg ugauuuugua gaagggauga gcggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac uguguccaau auggcugaag uacgcucaua cugcuaugag      780

gccaguauua gcgauauggc gucugacucc agauguccca cacaaggaga agccuaccuu      840

gacaaacagu cagauaccca guaugugugc aagaggacuu uggucgaccg gggcuggggu      900

aacgggugug gacuguucgg caaaggcagc cuagugacau gcgcaaaguu ugcuugcagu      960

aagaaaauga ccgggaaguc uauccagccu gagaaucucg aauaccgaau uaugcugucc     1020

guucacggaa gccaacacuc ggguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgcgcca aagucgaaau cacaccaaac ucucccagag cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu aggaccgggc ucgacuuuuc agaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagcgg cagaccguug ugguccuggg cagucaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cgccugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuuag gcugaaggga     1500

gucucuuaua gcuuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gccguuuaau cacagccaac     1680

ccaguuauua cggaaucaac cgagaauagu aagaugaugc uggagcuaga ucccccguuc     1740

ggagacuccu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgg     1800

agcggaucua caaucgguaa ggcuuuugaa gccaccgugc gaggcgcaaa aagaauggcu     1860

guacucgggg acacagccug ggauuucgga ucagugggcg gugcgcugaa cagucuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcu uguucggcgg gauguccugg     1980

uuuucucaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

ucgaucagcu ugaugugucu cgcauuaggg ggaguucuga uauuccuguc cacggccguc     2100

ucagcugacg ugggcugcag uguggauuuu ucuaaa                               2136


<210>  1760
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long CDS opt

<400>  1760
agaaucauua acgccaggaa ggagaaaaag cggcgcagcg cugacaccuc cgugggcaua       60

gucgggcugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg aucugcuuac      120

uauauguacc uggauagaaa ugacgccggu gaggcaaucu cauuccccac cacauugggc      180

augaacaaau gcuauauuca gaucauggau uuagggcaca ugugugacgc cacuaugagu      240

uacgaaugcc cuaugcugga ugagggagug gaaccagacg auguagacug uuggugcaau      300

accacaagca ccuggguggu cuauggcacu ugucaucaca agaaagguga ggcuaggcgg      360

ucccgcagag cagugacacu accgucucau ucgaccagga agcuccaaac gcggucacag      420

acuuggcugg agagccgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gguucgcccu ggcugccgcg gcaauagccu gguugcucgg aaguuccaca      540

ucucagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cagcauuagg      600

ugcaucggcg uguccaaccg ggauuuugua gaaggcaugu cagggggaac uuggguugac      660

guggucuuag agcauggugg cugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugaguaau auggcugaag uacguagcua cugcuaugag      780

gccucuauuu ccgauauggc gucagacagc cgcuguccca cacaagggga agccuaccuu      840

gacaaacaga gugauaccca guaugugugc aagagaacuu uggucgacag gggauggggc      900

aacgguugug ggcuguucgg aaaaggcucu cuagugacau gcgcaaaguu ugcuugcucc      960

aagaaaauga ccggcaagag cauccagccu gagaaucucg aauaccggau uaugcugucg     1020

guucacgggu cucaacacuc cggaaugauc gugaacgaua cgggucauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggcggg     1140

uucggaaguc ugggcuugga uugugaaccu agaaccgguc ucgacuuuag cgaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggggc cgauacuggc acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg aucccaggag     1380

ggcgccgugc auaccgcuuu ggccggggca cuagaagccg agauggaugg agcuaagggu     1440

cggcugucua gcggccaccu caaaugccgc cugaagaugg acaaacuuag acugaagggg     1500

gucucauaua guuuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggaacug ugaccguuga ggugcaguac gcuggcacag acgguccaug caagguaccc     1620

gcccaaaugg cagucgauac ccagacucug acaccugugg ggagguuaau cacggccaac     1680

ccaguuauua ccgaauccac ugagaauagc aagaugaugc uggagcuaga ucccccguuc     1740

ggcgacucuu acaucgugau aggagucggc gagaaaaaga uuacacacca cuggcaucgu     1800

ucagggagua ccaucggaaa ggcuuuugaa gccacagugc ggggugcaaa acgaauggcu     1860

guacucggcg acacugccug ggauuucggg agcgugggag gcgcgcugaa cucccuuggu     1920

aaagggaucc accagauuuu cggcgccgcu uuuaagucuu uguucggagg caugucgugg     1980

uuuagccaaa uccugauugg gacccuccug auguggcuug gacugaauac gaagaacggc     2040

uccauaucau ugaugugucu cgcacugggu gggguuuuaa ucuuccugag uaccgccguc     2100

ucugcugacg ugggaugcag cguggauuuu uccaaa                               2136


<210>  1761
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long CDS opt

<400>  1761
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccaa uguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uagcgcuuac      120

uauauguacc uggauagaaa cgacgccggc gaggcaaucu ccuuccccac cacauugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuaugucu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaucaa ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agucgcagag cagugacacu accgagccau uccaccagga agcuccaaac gcggucucag      420

acuuggcugg agucgcgcga auacaccaag caccuuauuc gaguugagaa uuggaucuuu      480

agaaaccccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cucaagcaca      540

agucagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cuccauuagg      600

ugcaucggcg ugucuaaucg ggauuuugua gaagggauga gcggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac uguguccaac auggcugaag uacguucaua cugcuaugag      780

gccaguauua gcgauauggc gucugacucc cgcuguccca cacaaggaga agccuaccuu      840

gacaaacagu cagauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcagc cuagugacau gcgcaaaguu ugcuugcagu      960

aagaaaauga ccgggaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

guucacggaa gccaacacuc ggguaugauc gugaaugaua cgggccauga gaccgacgag     1080

aaccgagcca aagucgaaau cacaccaaau ucuccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuuc agaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaacaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagucaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcugagcu ccgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucuuaua gcuuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau cacagccaau     1680

ccaguuauua cggaaucaac cgagaacagu aagaugaugc uggagcuaga ucccccguuc     1740

ggagacuccu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

agcggaucua caaucgguaa ggcuuuugaa gccaccgugc ggggcgcacg aagaauggcu     1860

guacucgggg acacagccug ggauuucgga ucagugggcg gugcgcugaa uagucuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcu uguucggcgg gauguccugg     1980

uuuucucaaa uccugauugg aacucuccug auguggcuug gccugaacac caaaaauggu     2040

ucgaucagcu ugaugugucu cgcauuaggg ggaguucuga uauuccuguc cacggccguc     2100

ucagcugacg ugggcugcag uguggauuuu ucuaag                               2136


<210>  1762
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long CDS opt

<400>  1762
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugaaaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggagguua cgcgacgugg uuccgcuuac      120

uauauguacc uggacagaaa ugaugccggc gaagcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggac uuaggacaca ugugugaugc cacuauguca      240

uacgagugcc cuaugcugga cgaaggcgug gagccagaug acguagauug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg aaucacgcga guacaccaag caccuuauuc gaguugaaaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggacuuugua gaggggaugu ccggagguac uuggguugau      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggacaagcc aacagucgau      720

auagaacugg uuaccacgac ugugucaaau auggcugagg uacguaguua cugcuaugaa      780

gccagcauuu cugacauggc guccgauuca cgcuguccca cacaaggaga ggccuaccuu      840

gacaaacaga gcgacaccca guaugugugc aagagaacuu uggucgauag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gaaaaucucg aguaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaca cgggccauga gaccgaugaa     1080

aaucgagcca aagucgagau cacaccaaac ucaccccgcg cugaagccac ucuuggggga     1140

uucggcuccc uggguuugga cugugagccu agaaccgggc ucgauuuuag ugaccuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag aaugguucca cgauauucca     1260

cucccguggc acgcaggcgc cgacacugga acaccccauu ggaauaacaa ggaggcucuu     1320

guagaguuua aagaugcgca cgcaaagagg cagaccguug ugguccuggg cagccaggaa     1380

ggggccgugc auaccgcuuu ggccggagca cuagaggccg aaauggacgg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg auaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggagacccuc     1560

cauggcacug ugaccguuga agugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgacau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggagaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugaa gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg auacagccug ggacuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aacggccguc     2100

agugcugaug ugggcugcuc uguggacuuu agcaaa                               2136


<210>  1763
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long CDS opt

<400>  1763
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuuuaagg      600

ugcauuggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

gugguccugg agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

aucgagcuug uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauau cugauauggc guccgacuca cgcuguccca cacaaggaga agccuacuug      840

gacaaacaga gcgauaccca guaugugugc aagagaacuc uggucgacag gggcuggggu      900

aacgggugug gacuauucgg caaaggcagu cucgugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucugg aauaccggau caugcuuagc     1020

guucacggau cgcaacacuc ugguaugauu gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucugggaggg     1140

uucggcuccu ugggucucga uugugaaccu agaaccgggc uggacuuuag ugauuuauau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacauccca     1260

cuuccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucug     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug uggucuuggg cagccaggag     1380

ggggccgugc auaccgcucu agccggagca cuggaagccg agauggaugg ugcuaagggc     1440

cggcucuccu cugggcaccu gaaaugccgc cuuaagaugg acaaacugag auugaaggga     1500

gucagcuauu cacucuguac ugccgcauuc acguuuacaa aaauuccugc ggaaacccug     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagaccuua acuccugugg gcaggcugau aacagccaac     1680

ccaguuauca cggaaaguac cgaaaauucc aagaugaugc uagagcucga ucccccguuc     1740

ggagacagcu acauugugau cggcgucggg gagaaaaaga uaacucacca cuggcaucgu     1800

ucuggaucaa caauugguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacuggggg acacagccug ggauuucgga agugugggcg gugcgcuuaa cagcuugggg     1920

aaaggcaucc accagaucuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuucgcaaa uucucaucgg aacucugcuu auguggcugg gcuugaauac caagaacggu     2040

agcauuuccc ucaugugucu ggcauuaggg ggaguucuga uauuccuauc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1764
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long CDS opt

<400>  1764
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccaa uguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uagcgcuuac      120

uauauguacc uggauagaaa cgacgccggc gaggcaaucu ccuuccccac cacauugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuaugucu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaucaa ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agucgcagag cagugacacu accgagccau uccaccagga agcuccaaac gcggucucag      420

acuuggcugg agucgcgcga auacaccaag caccuuauuc gaguugagaa uuggauguuu      480

agaaaccccg gauucgcccu ggcugccgcg gcaaucgccu gguugcucgg cucaagcaca      540

agucagaaag ugauauaucu ggucaugauc cugcucauug cuccugccua cuccaucagg      600

ugcauuggcg ugucuaaucg ggauuuugua gaagggauga gcggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

aucgagcugg uuaccacgac uguguccaac auggcugaag uacguucaua cugcuaugag      780

gccaguauaa gcgauauggc gucugacucc cgcuguccca cacaaggaga agccuaccuu      840

gacaaacagu cagauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcagc cuagugacau gcgcaaaguu ugcuugcagu      960

aagaaaauga ccgggaaguc uauucagccu gagaaccucg aauaccggau caugcugucc     1020

guucacggaa gccaacacuc ggguaugauu gugaaugaua cgggccauga gaccgacgag     1080

aaccgagcca aagucgaaau cacaccaaau ucuccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuuc agaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauccca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaacaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagucaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcugagcu ccgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucuuaua gcuuguguac ugccgcauuc acguuuacaa aaauuccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau aacagccaau     1680

ccaguuauca cggaaucaac cgagaacagu aagaugaugc uggagcuaga ucccccguuc     1740

ggagacuccu acauugugau cggcgucggg gagaaaaaga uaacucacca cuggcaucgu     1800

agcggaucua caauugguaa ggcuuuugaa gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacagccug ggauuucgga ucagugggcg gugcgcugaa uagucuuggg     1920

aaaggcaucc accagaucuu cggagccgcu uuuaagagcu uguucggcgg gauguccugg     1980

uuuucucaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

ucgauuagcu ugaugugucu cgcauuaggg ggaguucuga uauuccuguc cacggccguc     2100

ucagcugacg ugggcugcag uguggauuuu ucuaaa                               2136


<210>  1765
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long CDS opt

<400>  1765
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggauccug      480

agaaaucccg gauuugccuu ggcugccgcg gcaauagccu ggcuccuggg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cucuuaaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuucgua gaagggaugu ccggagguac uuggguugac      660

gugguccugg agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcuug uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuacuug      840

gacaaacaga gcgauaccca guaugugugc aagagaacuc uggucgacag gggcuggggu      900

aacgggugug gacuauuugg caaaggcagu cucgugacau gcgcaaaguu cgcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucugg aauaccggau uaugcuuagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucugggaggg     1140

uuuggcuccu ugggucucga uugugaaccu agaaccgggc uggacuucag ugauuuauau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguuuca cgacauucca     1260

cuuccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucug     1320

guagaguuca aagacgcgca cgcaaagagg cagaccguug uggucuuggg cagccaggag     1380

ggggccgugc auaccgcucu agccggagca cuggaagccg agauggaugg ugcuaagggc     1440

cggcucuccu cugggcaccu gaaaugccgc cuuaagaugg acaaacugag auugaaggga     1500

gucagcuauu cacucuguac ugccgcauuu acguucacaa aaauaccugc ggaaacccug     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagaccuua acuccugugg gcaggcugau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aagaugaugc uagagcucga ucccccguuu     1740

ggagacagcu acaucgugau aggcauuggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuucgag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcuggggg acacagccug ggauuuugga aguguaggcg gugcgcuuaa cagcuugggg     1920

aaaggcauuc accagaucuu cggagccgcu uucaaguccc uguuuggcgg gaugucuugg     1980

uucucgcaaa uucucauagg aacucugcuu auguggcugg gcuugaauac caagaacggu     2040

agcaucuccc ucaugugucu ggcauuaggg ggagugcuga uuuuucuauc aacggccguu     2100

agugcugacg ucggcugcuc uguggauuuc agcaaa                               2136


<210>  1766
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long CDS opt

<400>  1766
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuucaaccac ggugaguaau auggcugaag uacguagcua cugcuaugag      780

gccacuauuu cugauaucgc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccgccg uugugcuggg cagccaggag     1380

ggggcugucc auaccgccuu ggcaggagcc cuagaagcug agauggaugg ugccaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugcagcguuc acguuuacaa aaaucccugc ugaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gccgguacag acgggccaug caaggugccc     1620

gcacaaaugg ccguagauau gcagacccug acuccugucg gcagguuaau uacagcuaac     1680

ccagugauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa agccuuugag gcaaccgucc ggggcgcuaa gcgaauggcc     1860

gugcucgggg acacagcgug ggauuucgga aguguaggcg gugcccugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagcugca uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgccuuaggg ggagugcuga uuuuccuguc aacggcuguu     2100

agugccgacg ucggcugcuc uguggauuuu agcaaa                               2136


<210>  1767
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long CDS opt

<400>  1767
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac agcccccgcg cugaggccac ucuuggggga     1140

uucggcucac uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cacccaggag     1380

ggggccgugc auacugcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcugagcu ccgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucuuaua gcuuguguac ggccgcauuc acauuuacca aaauaccugc ggaaacucuc     1560

cauggcaccg ugacaguuga ggugcaguac gcugguaccg acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacucug acaccugugg gcagguuaau cacggccaac     1680

ccaguuauua ccgaaucaac ugagaauagu aagaugaugc uggagcuaga ucccccguuc     1740

ggagacuccu acaucgugau aggcgucggg gagaaaaaga uuacacacca cuggcaucgu     1800

agcggaucua ccaucgguaa ggcuuuugaa gccacagugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacugccug ggauuucgga ucagugggcg gugcgcugaa cagucuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcu uguucggcgg gauguccugg     1980

uuuucucaaa uccugauugg aacccuccug auguggcuug gccugaauac gaagaacggu     2040

ucgauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc caccgccguc     2100

ucagcugacg ugggcugcag uguggauuuu ucuaaa                               2136


<210>  1768
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long CDS opt

<400>  1768
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga gcugcaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagaccuua acuccugucg gcaggcugau cacagccaac     1680

ccagugauua cggaaaguac cgaaaauucc aagaugaugc uagagcucga ucccccguuc     1740

ggagacagcu acaucguuau aggcgugggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcuggggg acacagccug ggauuucgga aguguaggcg gugcgcuuaa cagcuugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuucgcaaa uccucauugg aacucugcuu auguggcugg gcuugaauac caagaacggu     2040

agcauauccc ucaugugucu ggcauuaggg ggagugcuga ucuuccuauc aacggccguu     2100

agugcugacg ucggcugcuc uguggauuuu agcaaa                               2136


<210>  1769
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long CDS opt

<400>  1769
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugaaaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggagguua cgcgacgugg uuccgcuuac      120

uauauguacc uggacagaaa ugaugccggc gaagcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggac uuaggacaca ugugugaugc cacuauguca      240

uacgagugcc cuaugcugga cgaaggcgug gagccagaug acguagauug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg aaucacgcga guacaccaag caccuuauuc gaaucgaaaa cuggauauuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaaucgccu gguugcucgg cagcaguaca      540

ucccagaaag uuauuuaucu ggugaugauc cugcucauug cuccugccua cucuaucagg      600

ugcauaggcg ucagcaaccg ggacuuugug gaggggaugu ccggagguac uuggguagau      660

guuguguuag agcauggcgg gugugucacc gugauggcac aggacaagcc aacaguggau      720

auugaacugg ucaccacgac uguuucaaau auggcugagg ugcguaguua cugcuaugaa      780

gccagcaucu cugacauggc guccgauuca cgcuguccca cacaaggaga ggccuaccuu      840

gacaaacaga gcgacaccca guauguaugc aagagaacuu ugguggauag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gaaaaucucg aguaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaca cgggccauga gaccgaugaa     1080

aaucgagcca aaguggagau uacaccaaac ucaccccgcg cugaagccac ucuuggggga     1140

uucggcuccc uggguuugga cugugagccu agaaccgggc ucgauuuuag ugaccuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag aaugguucca cgauauacca     1260

cucccguggc acgcaggcgc cgacacugga acaccccauu ggaauaacaa ggaggcucuu     1320

guggaguuua aagaugcgca cgcaaagagg cagaccguag uugugcuggg cagccaggaa     1380

ggggccgucc auaccgcuuu ggccggagca cuagaggccg aaauggacgg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg auaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggagacccuc     1560

cauggcacug ucaccgugga aguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagacau gcagacccug acuccugucg gcagguuaau uacagccaac     1680

ccagugauca cggagaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauaguuau uggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugaa gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg auacagccug ggacuucgga aguguaggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggagugcuga uuuuccuguc aacggccguu     2100

agugcugaug ucggcugcuc uguggacuuu agcaaa                               2136


<210>  1770
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long CDS opt

<400>  1770
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggaaaguau cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauagugau uggcgucggg gagaaaaaga ucacccacca cuggcaucgu     1800

ucuggaucaa cuaucgguaa ggcuuuugag gccacagugc ggggcgcaaa acgaauggcu     1860

guacucgggg acaccgccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacacuccug auguggcuug gccugaauac uaagaacggu     2040

agcaucuccu ugaugugucu cgcauuaggg ggaguucuga uuuuccuguc aaccgccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1771
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long CDS opt

<400>  1771
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugucaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauaccugc agaaacccuc     1560

cauggcacug uuaccgugga gguacaguac gcggguacag acgggccaug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acuccuguug gcagguuaau cacagcaaac     1680

ccagugauua cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgucau aggcgugggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa agccuuugag gcuaccguac ggggcgccaa gcgaauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguuggcg gugcccugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ucauguguuu ggcucucggg ggaguccuga ucuuuuuauc aacggcagug     2100

agugccgacg ugggcugcuc ugucgauuuc agcaaa                               2136


<210>  1772
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long CDS opt

<400>  1772
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

aaacuguccu cugggcaccu caagugccgg cugaaaaugg acaagcugcg ccugaaagga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa agauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagauuaau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aaaaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaagaaaa uuacucacca cuggcauagg     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc guggcgcaaa acggauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aagggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caaaaacggu     2040

agcauauccu ugaugugucu cgcacuuggg ggaguuuuaa ucuuccuguc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaag                               2136


<210>  1773
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long CDS opt

<400>  1773
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagaggcgca cgcaaagagg cagaccguug ugguccuggg cagccaggaa     1380

ggggccgugc auaccgcuuu ggccggagca cuagaggccg aaauggacgg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg auaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggagacccuc     1560

cauggcacug ugaccguuga agugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgacau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggagaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugaa gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg auacagccug ggacuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aacggccguc     2100

agugcugaug ugggcugcuc uguggacuuu agcaaa                               2136


<210>  1774
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long CDS opt

<400>  1774
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcuggugccg acgggccaug caagguaccc     1620

gcacaaaugg ccgucgauau gcagacacug accccugugg gcagguuaau cacugcuaac     1680

ccaguuauua cagaaaguac ggaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacccacca cuggcaucgu     1800

ucuggaucaa cuaucgguaa agccuuugag gcaacagugc ggggcgcuaa gcgaauggcc     1860

guacucgggg acaccgcgug ggauuucgga agugugggcg gugcccugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagcugca uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacacuccug auguggcuug gccugaauac uaagaacggu     2040

agcauauccu ugaugugucu cgccuuaggg ggaguucuga ucuuccuguc aaccgcuguc     2100

agugccgacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1775
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long CDS opt

<400>  1775
agaaucauua acgccggcaa ggagaaaaag aggcgcgggg cugacaccaa ugugggaaua       60

gucggucugc uccuuacaac ugcaauggcc gcggaaguua cgcggcgagg cagcgcuuac      120

uauauguacc uggaucguaa cgacgccggg gaggcaaucu ccuuccccac cacauuggga      180

augaauaaau gcuauauuca gaucauggau uuaggccaca ugugugacgc cacuaugucu      240

uacgaaugcc cuaugcugga ugagggugug gaaccagacg auguagacug uuggugcaac      300

accacaucaa ccuggguggu cuaugggacu ugucaucaca agaaaggaga ggcuagaagg      360

agucggcgcg cagugacacu accgagccau uccaccagaa agcuccaaac gaggucucag      420

acuuggcugg agucgcggga auacaccaag caccuuauuc gcguugagaa uuggaucuuu      480

cgaaaccccg gcuucgcccu ggcugccgcg gcaauagccu gguugcucgg cucaagcaca      540

agucagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cuccauuaga      600

ugcaucgggg ugucuaauag ggauuuugua gaaggaauga gcgguggcac uuggguugac      660

guggucuuag agcauggggg augugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac uguguccaac auggcugaag uacggucaua cugcuaugag      780

gccaguauua gcgauauggc gucugacucc cguuguccca cacaaggcga agccuaccuu      840

gacaaacagu cagauaccca guaugugugc aagcgcacuu uggucgacag agguuggggg      900

aauggaugug gccuguucgg caaagggagc cuagugacau gcgcaaaguu ugcuugcagu      960

aagaaaauga ccggaaaguc uauccagccu gagaaccucg aauacaggau uaugcugucc     1020

guucacggua gccaacacuc gggcaugauc gugaaugaua cggggcauga gaccgacgag     1080

aaccgggcca aagucgaaau cacaccaaau ucuccccgag cugaggccac ucuuggaggc     1140

uucgguuccc ugggguugga uugugaaccu cgcaccggcc ucgacuuuuc agaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggagc cgauacuggc acaccccauu ggaacaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagaga cagaccguug ugguccuggg gagucaggag     1380

ggagccgugc auaccgcuuu ggccggugca cuagaagccg agauggaugg cgcuaagggg     1440

aggcugagcu ccggacaccu caaaugccgg cugaagaugg acaaacuucg ccugaagggc     1500

gucucuuaua gcuuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

caugguacug ugaccguuga ggugcaguac gcugggacag acggcccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gaagauuaau cacagccaau     1680

ccaguuauua cggaaucaac cgagaacagu aagaugaugc uggagcuaga ucccccguuc     1740

ggcgacuccu acaucgugau aggggucgga gagaaaaaga uuacucacca cuggcauagg     1800

agcgguucua caaucggcaa ggcuuuugaa gccaccgugc guggggcacg gcgaauggcu     1860

guacucggag acacagccug ggauuucggc ucagugggug gggcgcugaa uagucuuggc     1920

aaaggaaucc accagauuuu cggcgccgcu uuuaagagcu uguucggggg aauguccugg     1980

uuuucucaaa uccugauugg cacucuccug auguggcuug gucugaacac caaaaauggg     2040

ucgaucagcu ugaugugucu cgcacuggga ggcguuuuaa uauuccuguc cacggccguc     2100

ucagcugacg uggguugcag uguggauuuu ucuaag                               2136


<210>  1776
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long CDS opt

<400>  1776
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugaaaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggagguua cgcgacgugg uuccgcuuac      120

uauauguacc uggacagaaa agaugccggc gaagcaaucu cuuuccccac cacauugggg      180

augaauaagu gcuauauuca gaucauggac uuaggacaca ugugugaugc cacuauguca      240

uacgagugcc cuaugcugga cgaaggcgug gagccagaug acguagauug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca aaaaggggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg aaucacgcga guacaccaaa caccuuauuc gaguugaaaa uuggaucuuu      480

agaaaccccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaagg ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaucg ggacuuugua gaggggaugu ccggagguac uuggguugau      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggacaaacc aacagucgau      720

auagaacugg uuaccacgac ugugucaaac auggcugagg uacguaguua cugcuaugaa      780

gccagcauuu cugacauggc guccgauuca cgcuguccca cacaaggaga ggccuaccuu      840

gacaagcaga gcgacaccca guaugugugc aaaagaacuu uggucgauag gggcuggggu      900

aaugggugug gacuguucgg caagggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aaaaagauga ccgggaaauc cauccagccu gaaaaccucg aguaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaaugaca cgggccauga gaccgaugaa     1080

aaccgagcca aggucgagau cacaccaaau ucaccccgcg cugaagccac ucuuggggga     1140

uucggcuccc uggguuugga cugugagccu agaaccgggc ucgauuuuag ugaccuguau     1200

uacuuaacaa ugaacaauaa acacuggcug gugcauaagg aaugguucca cgauauucca     1260

cucccguggc acgcaggcgc cgacacugga acaccccauu ggaacaauaa agaggcucuu     1320

guagaguuua aggaugcgca cgcaaagagg cagaccguug ugguccuggg cagccaggaa     1380

ggggccgugc auaccgcuuu ggccggagca cuagaggccg aaauggacgg ugcuaaaggc     1440

cggcuguccu cugggcaccu caagugccgc cugaaaaugg auaagcugag acugaaagga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa agauaccugc ggagacccuc     1560

cauggcacug ugaccguuga agugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgacau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggagaguac cgagaacucc aaaaugaugc uggaacuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaagaaaa uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugaa gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg auacagccug ggacuucgga agugugggcg gugcgcugaa uagccuuggg     1920

aagggcaucc accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaacac caaaaauggu     2040

agcauauccu ugaugugucu cgcacuuggg ggaguuuuaa ucuuccuguc aacggccguc     2100

agugcugaug ugggcugcuc uguggacuuu agcaag                               2136


<210>  1777
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long CDS opt

<400>  1777
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggu guccgacuca cgcuguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guaugucugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuaguuacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc gucaacgaua cgggccauga gaccgacgag     1080

aaucgagcua aaguggaaau cacaccaaac ucaccccgcg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guacauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaauaacaa ggaagcccuu     1320

guugaguuua aagacgcuca cgcgaagagg cagaccgugg ucgugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauaccugc agaaacccuc     1560

cauggcacug uuaccgugga gguacaguac gcggguacag acgggccaug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acuccuguug gcagguuaau cacagcaaac     1680

ccagugauua cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgucau aggcgugggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa agccuuugag gcuaccguac ggggcgccaa gcgaauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguuggcg gugcccugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcuuuaggg ggaguccuga ucuuccuguc aacggcagug     2100

agugccgacg ugggcugcuc ugucgauuuu agcaaa                               2136


<210>  1778
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long CDS opt

<400>  1778
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccaa uguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uagcgcuuac      120

uauauguacc uggauagaaa cgacgccggc gaggcaaucu ccuuccccac cacauugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuaugucu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

augaccucaa cauggguggu cuaugguacc ugucaucaca agaaagggga ggcuaggcgg      360

agucgcagag cagugacucu accgagccau uccacaagga agcuccaaac ccggucucag      420

acguggcugg agucgcgcga auacacuaag caccuuauuc gaguugagaa uuggaucuuu      480

agaaaccccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cucaagcacc      540

agucagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cuccauuagg      600

ugcaucggcg ugucuaaucg ggauuuugua gaagggauga gcggagguac auggguugac      660

guggucuuag agcauggcgg gugugugacu gugauggcac aggauaagcc aaccgucgac      720

auagagcugg uuacaaccac gguguccaac auggcugaag uacguucaua cugcuaugag      780

gccaguauua gcgauauggc gucugacucc cgcuguccca cucaaggaga agccuaccuu      840

gacaaacagu cagauacaca guaugugugc aagagaaccu uggucgacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcagc cuagugacuu gcgcaaaguu ugcuugcagu      960

aagaaaauga cagggaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

guucacggaa gccaacacuc ggguaugauc gugaaugaua ccggccauga gacggacgag     1080

aaccgagcca aagucgaaau caccccaaau ucuccccgcg cugaggccac acuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaacugggc ucgacuuuuc agaucuguau     1200

uacuuaacca ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacagga acuccccauu ggaacaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagucaggag     1380

ggggccgugc auacagcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcugagcu ccgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucuuaua gcuuguguac cgccgcauuc acuuuuacga aaauaccugc ggaaacacuc     1560

cauggcaccg ugacuguuga ggugcaguac gcugguaccg acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacacug accccugugg gcagguuaau cacugccaau     1680

ccaguuauua cagaaucaac ggagaacagu aagaugaugc uggagcuaga ucccccguuc     1740

ggagacuccu acaucgugau aggcgucggg gagaaaaaga uuacccacca cuggcaucgu     1800

agcggaucua cuaucgguaa ggcuuuugaa gccacagugc ggggcgcaaa acgaauggcu     1860

guacucgggg acaccgccug ggauuucgga ucagugggcg gugcgcugaa uagucuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcu uguucggcgg gauguccugg     1980

uuuucucaaa uccugauugg aacacuccug auguggcuug gccugaacac uaagaauggu     2040

ucgaucagcu ugaugugucu cgcauuaggg ggaguucuga uauuccuguc caccgccguc     2100

ucagcugacg ugggcugcag uguggauuuu ucuaaa                               2136


<210>  1779
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long CDS opt

<400>  1779
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau cacaccaaac ucaccccgcg cugaggccgc acuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaacugggc ucgacuuuag ugaucuguau     1200

uacuuaacca ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc ugauacagga acuccccauu ggaauaacaa ggaagcgcuu     1320

guagaguuua aagacgcaca cgccaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggcugugc auacagccuu ggcaggagcc cuagaagcug agauggaugg ugccaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac cgcagcguuc acuuuuacga aaauaccugc ugaaacacuc     1560

cauggcaccg ugacuguuga ggugcaguac gccgguaccg acgggccaug caagguaccc     1620

gcacaaaugg ccgucgauau gcagacacug accccugugg gcagguuaau cacugcuaac     1680

ccaguuauua cagaaaguac ggaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacccacca cuggcaucgu     1800

ucuggaucaa cuaucgguaa agccuuugag gcaacagugc ggggcgcuaa gcgaauggcc     1860

guacucgggg acaccgcgug ggauuucgga agugugggcg gugcccugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagcugca uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa cacugaucgg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauuuccu ugaugugucu cgccuuaggg ggaguucuga uauuccuguc aacggcuguc     2100

agugccgacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1780
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long CDS opt

<400>  1780
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugaaaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggagguua cgcgacgugg uuccgcuuac      120

uauauguacc uggacagaaa ugaugccggc gaagcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggac uuaggacaca ugugugaugc cacuauguca      240

uacgagugcc cuaugcugga cgaaggcgug gagccagaug acguagauug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg aaucacgcga guacaccaag caccuuauuc gaguugaaaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuuuagccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggacuuugua gaggggaugu ccggagguac uuggguugau      660

gugguccugg agcauggcgg gugugugacc gugauggcac aggacaagcc uacagucgau      720

auagaacuug uuaccacgac ugugucaaau auggcugagg uacguaguua cugcuaugaa      780

gccagcauuu cugacauggc guccgauuca cgcuguccaa cacaaggaga ggccuacuug      840

gacaaacaga gcgacaccca guaugugugc aagagaacuc uggucgauag gggcuggggu      900

aacgggugug gacuauucgg caaaggcagu cucgugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccagccc gaaaaucugg aguaccggau uaugcuuagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaca cgggccauga gaccgaugaa     1080

aaucgagcca aagucgagau cacaccuaac uccccacgcg cugaagccac ucugggaggg     1140

uucggcucau ugggucucga cugugagccc agaaccgggc uggauuuuag ugacuuauau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag aaugguucca cgauauuccu     1260

cuuccauggc acgcaggcgc cgacacugga accccgcauu ggaauaacaa ggaggcucug     1320

guagaguuua aagaugcgca cgcaaagagg cagacaguug uggucuuggg cagccaggaa     1380

ggggccgugc auaccgcucu agccggagca cuggaggccg aaauggacgg ugcuaagggc     1440

cggcucuccu cugggcaccu gaaaugccgc cuuaagaugg auaaacugag auugaaggga     1500

gucagcuauu cacucuguac ugccgcauuc acguuuacaa aaauacccgc ggagacccug     1560

cauggcacug ugaccguuga agugcaguac gcugguacag acgggccuug caagguacca     1620

gcccaaaugg cagucgacau gcagaccuua acucccgugg gcaggcugau cacagccaac     1680

ccuguuauua cggagaguac cgaaaauucc aagaugaugc uagagcucga uccacccuuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugaa gccaccgugc ggggcgcaaa acgaauggcu     1860

guacuggggg auacagccug ggacuucgga agugugggcg gugcgcuuaa cagcuugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuucgcaaa uccucauugg aacucugcuu auguggcugg gcuugaauac caagaacggu     2040

agcauauccc ucaugugucu ggcauuaggg ggaguucuga ucuuccuauc aacggccguc     2100

agugcugaug ugggcugcuc uguggacuuu agcaaa                               2136


<210>  1781
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long CDS opt

<400>  1781
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggca uaagcaaccg ggauuuugug gaagggaugu ccggagguac uuggguagac      660

guuguguuag agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac      720

auugagcugg ucaccacgac uguuucaaau auggcugaag ugcguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aaguggaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcauac uggguuugga uugugaaccu agaaccgggc ucgacuuuuc cgaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guggaguuua aagacgcgca cgcaaagagg cagaccguag uugugcuggg cagucaggag     1380

ggggccgucc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcugagcu ccgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugucuuaua gcuuguguac ugccgcauuc acguuuacaa aaauuccugc ggaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc     1620

gcccaaaugg caguagauau gcagacccug acuccugucg gcagguuaau cacagccaac     1680

ccagugauaa cggaaucaac cgagaauagu aagaugaugc uggagcuaga ucccccguuc     1740

ggagacuccu acauuguuau cggcgugggg gagaaaaaga ucacucacca cuggcaucgu     1800

agcggaucua caauugguaa ggcuuuugaa gccaccgucc ggggcgcaaa acgaauggcu     1860

gugcucgggg acacagccug ggauuucgga ucaguaggcg gugcgcugaa cagucuuggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcu uguucggcgg gauguccugg     1980

uuuucucaaa uacugaucgg aacucuccug auguggcuug gccugaauac caagaacggu     2040

ucgauuagcu ugaugugucu cgcauuaggg ggagugcuga ucuuccuguc cacggccguu     2100

ucagcugacg ucggcugcag uguggauuuu ucuaaa                               2136


<210>  1782
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long CDS opt

<400>  1782
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca cuugugacgc caccauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

acaaccagua cuuggguggu cuaugguaca ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacccu accgucccau ucuauuagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuaucc gaguugagaa cuggauauuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaaucgccu gguugcucgg cagcaguaca      540

ucccagaaag ugauuuaucu ggucaugauc cugcucauug cuccugccua cucuaucagg      600

ugcauaggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auugagcugg uuaccacgac ugugucaaau auggcugaag uacguaguua cugcuaugag      780

gccagcaucu cugauauggc guccgacuca cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaucucg aauaccggau caugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgagcca aagucgaaau uacaccaaac ucaccccgcg cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauacca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaaucccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau uacagccaac     1680

ccaguuauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauagugau uggcgucggg gagaaaaaga ucacucacca cuggcaucgu     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc ggggcgcaaa acgaauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugauagg aacucuccug auguggcuug gccugaaugc aaagaacggu     2040

agcaucuccu ugaugugucu cgccuuaggg ggaguucuga uuuuccuguc aaccgcuguc     2100

agugccgacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1783
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long CDS opt

<400>  1783
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcucaaagg      360

agccggcgcg cagugacacu accgucccau ucuaccagaa agcuccagac gaggucgcag      420

acuuggcugg agucacggga auacaccaag caccuuauuc gcguugagaa cuggaucuuu      480

cgaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuaga      600

ugcaucggcg ugagcaacag ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aagauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugucaaau auggcugaag uacggaguua cugcuaugag      780

gccagcauuu cugauauggc guccgacuca cguuguccca cacagggaga agccuaccuu      840

gacaaacaga gcgauaccca guaugugugc aagcgcacuu uggucgacag aggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacau gcgcaaaguu ugcuugcucu      960

aagaaaauga ccgggaaguc cauccaaccu gagaaucucg aauacaggau uaugcugagc     1020

guucacggau cgcagcacuc ugguaugauc gugaacgaua cgggccauga gaccgacgag     1080

aaucgggcca aagucgaaau cacaccaaac ucaccccgag cugaggccac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgcaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagaga cagaccguug ugguccuggg cagccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

aggcuguccu cugggcaccu caaaugccgg cugaagaugg acaaacuucg ccugaaggga     1500

gucagcuauu cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaauac gcugguacag acgggccaug caagguaccc     1620

gcccagaugg cagucgauau gcagacccug acuccugugg gcagauuaau cacagccaac     1680

ccaguuauua cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcauagg     1800

ucuggaucaa caaucgguaa ggcuuuugag gccaccgugc guggcgcaaa acggauggcu     1860

guacucgggg acacagccug ggauuucgga agugugggcg gugcgcugaa cagccuuggg     1920

aaaggcaucc accaaauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaga uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu     2040

agcauauccu ugaugugucu cgcauuaggg ggaguucuga ucuuccuguc aacggccguc     2100

agugcugacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1784
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long CDS opt

<400>  1784
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaagc ccggucgcag      420

acguggcugg agucacgcga auacacuaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcucu ggccgcggca gccauagcuu gguugcucgg cagcaguacc      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg ccccugcaua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac auggguugac      660

guggucuuag agcauggcgg gugugugacu gugauggcuc aggauaagcc aaccgucgac      720

auagagcugg uuacaaccac ggugucaaau auggccgaag uacguaguua cugcuaugag      780

gcgagcauuu cugauauggc cuccgacuca cgcuguccca cucaaggaga agcauaccuu      840

gacaaacaga gcgauacaca guaugugugc aagagaaccu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagugacuu gcgcuaaguu ugccugcucu      960

aagaaaauga cagggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

guucacggau cgcaacacuc ugguaugauc gugaacgaua ccggccauga gacggacgag     1080

aaucgagcua aagucgaaau caccccaaac ucaccccgcg ccgaggcaac acuuggggga     1140

uucggcuccc uggguuugga uugugaaccu agaacugggc ucgacuuuag ugaucuguau     1200

uacuuaacca ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc ugauacagga acuccccauu ggaauaacaa ggaagcgcuu     1320

guagaguuua aagacgcaca cgccaagagg cagaccguug ugguccuggg cagccaggag     1380

ggggcugugc auacagccuu ggcaggagcc cuagaagcug agauggaugg ugccaagggc     1440

cggcuguccu cugggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cauuguguac cgcagcguuc acuuuuacga aaauaccugc ugaaacacuc     1560

cauggcaccg ugacuguuga ggugcaguac gccgguaccg acgggccaug caagguaccc     1620

gcacaaaugg ccgucgauau gcagacacug accccugugg gcagguuaau cacugcuaac     1680

ccaguuauua cagaaaguac ggaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acaucgugau aggcgucggg gagaaaaaga uuacccacca cuggcaucgu     1800

ucuggaucaa cuaucgguaa agccuuugag gcaacagugc ggggcgcuaa gcgaauggcc     1860

guacucgggg acaccgcgug ggauuucgga agugugggcg gugcccugaa cagccuuggg     1920

aaaggcaucc accagauuuu cggagcugca uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uccugauugg aacacuccug auguggcuug gccugaauac uaagaacggu     2040

agcauauccu ugaugugucu cgccuuaggg ggaguucuga ucuuccuguc aaccgcuguc     2100

agugccgacg ugggcugcuc uguggauuuu agcaaa                               2136


<210>  1785
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long CDS opt

<400>  1785
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagauc ugacgccggc gaggcaaucu cauuccccac cacauugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuaugagu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagca ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

ucccgcagag cagugacacu accgucucau ucgaccagga agcuccaaac gcggucacag      420

acuuggcugg agagccgcga auacaccaag caccuuauuc gaguugagaa uuggaucuuu      480

agaaaccccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg caguuccaca      540

ucucagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cagcauuagg      600

ugcaucggcg uguccaaucg ggauuuugua gaagggaugu caggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gugauggcac aggauaagcc aacagucgac      720

auagagcugg uuaccacgac ugugaguaac auggcugaag uacguagcua cugcuaugag      780

gccucuauuu ccgauauggc gucagacagc cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gugauaccca guaugugugc aagagaacuu uggucgacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcucu cuagugacau gcgcaaaguu ugcuugcucc      960

aagaaaauga ccgggaaaag cauccagccu gagaaccucg aauaccggau uaugcugucg     1020

guucacggau cucaacacuc cgguaugauc gugaaugaua cgggccauga gaccgacgag     1080

aaccgagcca aagucgaaau cacaccaaau ucaccccgcg cugaggccac ucuuggggga     1140

uucggcaguc uggguuugga uugugaaccu agaaccgggc ucgacuuuag cgaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgcaggcgc cgauacugga acaccccauu ggaacaauaa ggaagcucuu     1320

guagaguuua aagacgcgca cgcaaagagg cagaccguug ugguccuggg cucccaggag     1380

ggggccgugc auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc     1440

cggcugucua gcgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucauaua guuuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc     1560

cauggcacug ugaccguuga ggugcaguac gcugguacag acgggccaug caagguaccc     1620

gcccaaaugg cagucgauau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccaguuauua cggaauccac cgagaacagc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacucuu acaucgugau aggcgucggg gagaaaaaga uuacucacca cuggcaucgu     1800

ucaggaagua caaucgguaa ggcuuuugaa gccaccgugc ggggcgcaaa gcgaauggcu     1860

guacucgggg acacagccug ggauuucgga agcgugggcg gugcgcuuaa uucccugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagucuu uguucggcgg gaugucgugg     1980

uuuagccaaa uccugauugg aacucuccug auguggcuug gccugaacac caagaauggu     2040

uccauaucau ugaugugucu cgcauuaggg ggaguucuga ucuuccugag uacggccguc     2100

ucugcugacg ugggcugcag cguggauuuu uccaaa                               2136


<210>  1786
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long CDS opt

<400>  1786
agaaucauua acgccaggaa ggagaaaaag cggcgcggca ccgacacaag cguggggaua       60

gucggacugc uccuuacuac ggcuauggca gccgaaguua cccgacgugg uuccgcguac      120

uauauguacc uggauagauc ugacgcuggc gaggccaucu cauuccccac aacuuugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc aaccaugagu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

acaaccagca cuuggguggu cuaugguaca ugucaucaca agaaagggga ggccaggcgg      360

ucccgcagag cugugacccu accgucucau ucgacgagga agcuccaaac ucggucacag      420

accuggcugg agagccgcga auacacaaag caccuuauuc gaguugagaa uuggaucuuu      480

agaaaccccg gauucgcacu ggccgcugcc gcgauagcau gguugcucgg caguuccacu      540

ucucagaaag ugaucuaucu ggucaugauu cugcucaucg ccccugcuua cagcauuagg      600

ugcaucggcg uguccaaucg ggauuuugua gaagggaugu caggagguac cuggguugac      660

guggucuuag agcauggcgg gugugugaca gugauggccc aggauaagcc aaccgucgac      720

auagagcugg uuacgacuac agugaguaac auggcagaag uacguagcua cugcuaugag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccca cccaaggaga agcguaccuu      840

gacaaacaga gugauacuca guaugugugc aagagaacau uggucgacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcucu cuagugaccu gcgccaaguu ugcaugcucc      960

aagaaaauga cggggaaaag cauccagccu gagaaccucg aauaccggau uaugcugucg     1020

guucacggau cucaacacuc cgguaugauc gugaaugaua ccggccauga gacagacgag     1080

aaccgagcua aagucgaaau cacuccaaau ucaccccgcg ccgaggcuac ccuuggggga     1140

uucggcaguc uggguuugga uugugaaccu agaacagggc ucgacuuuag cgaucuguau     1200

uacuuaacua ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acaccggcgc cgauacagga acuccccauu ggaacaauaa ggaagcacuu     1320

guagaguuua aagacgccca cgcuaagagg cagaccguug ugguccuggg cucccaggag     1380

ggggcggugc auacggcauu ggccggagcu cuagaagccg agauggaugg ugcaaagggc     1440

cggcugucua gcgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucauaua guuuguguac agccgcuuuc accuuuacua aaauaccugc cgaaacccuc     1560

cauggcacag ugaccguuga ggugcaguac gcagguacug acgggccaug caagguaccc     1620

gcgcaaaugg cugucgauau gcagacacug acgccugugg gcagguuaau caccgccaac     1680

ccaguuauua cugaaggaac agagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggcgacagcu acaucgugau aggggucgga gaaaagaaga uuacccacca cuggcaucgu     1800

ucugguucaa caaucggcaa agcauuugag gccacugugc gaggggcuaa gcggauggcc     1860

guacucggag acaccgcaug ggauuucggc agugugggug ggguucugaa uagccuuggc     1920

aaaggaaucc accagauuuu cggcgcugcc uuuaaguccu uguucggggg aaugucuugg     1980

uuuucgcaaa uccugauugg cacgcuccug auguggcuug gucugaacac caagaauggg     2040

agcauauccu ugaugugucu cgcgcuggga ggcgucuuaa ucuuccuguc aacagccgug     2100

agugcugacg uggguugcuc ugucgauuuu agcaaa                               2136


<210>  1787
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long CDS opt

<400>  1787
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc gcggaaguua cgcgacgugg uuccgcuuac      120

uauauguacc uggauagaaa ugacgccggc gaggcaaucu cuuuccccac cacauugggg      180

augaacaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc cacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuaggcgg      360

agccgcagag cagugacacu accgucccau ucuaccagga agcuccaaac gcggucgcag      420

acuuggcugg agucacgcga auacaccaag caccuuauuc gaguugagaa cuggaucuuu      480

agaaaucccg gauucgcccu ggcugccgcg gcaauagccu gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugauu cugcucaucg cuccugccua cucuauuagg      600

ugcaucggcg ugagcaaccg ggauuuugua gaagggaugu ccggagguac uuggguugac      660

guggucuuag agcauggcgg gugugugacc gcaauggcuc aggauaagcc aacaguggac      720

auagagcugg ucaccacgac uguuucaaau auggccgaag ugcguaguua cugcuaugag      780

gcgagcauuu cugauauggc cuccgacuca cgcuguccca cacaaggaga agcauaccuu      840

gacaaacaga gcgauaccca guauguaugc aagagaacuu ugguggacag gggcuggggu      900

aacgggugug gacuguucgg caaaggcagu cuagucacau gcgcuaaguu ugccugcucu      960

aagaaaauga ccgggaaguc cauccagccu gagaaucucg aauaccggau uaugcugagc     1020

gugcacggau cgcaacacuc ugguaugcuu guuaacgaua cgggccauga gaccgacgag     1080

aaucgagcua aaguggaaau cacaccaaac ucaccccgcg ccgaggcaac ucugggaggg     1140

uucggcuccu ugggucucga uugugaaccu agaaccgggc uggacuuuag ugauuuauau     1200

uaccugacaa ugaauaacaa gcacuggcuc gcccauaaag agugguucca cgacauccca     1260

cuuccguggc acgcuggcgc ggcaacugga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagaugcuca cgccaagagg cagaccgugg uaguuuuggg cagccaggag     1380

ggggcagugc auaccgcccu agcuggagcc cuggaagcag agauggacgg ugcgaagggc     1440

cggcucuccu cugggcaccu gaaaugccgc cuuaagaugg auaaacugag auugaaggga     1500

gucagcuauu cacucuguac ugcugccuuc acguuuacaa aaauuccugc agaaaccgug     1560

gacggcacug ucaccgugga gggucaguac gggggcacag acggaccaug caagguuccc     1620

gcccaaaugg cuguggauau gcagacccug acuccuguag gcagguuaau aacagccaac     1680

ccagucauca cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc     1740

ggggacagcu acauugugau cggaguuggu gagaaaaaga uaacucauca cuggcaccgu     1800

ucuggcucaa caauugggaa agcauuugag gcuaccgugc ggggagccaa gcgaauggcg     1860

guccucggcg acacagccug ggauuucggu aguguagggg gcgcucugaa cagccuugga     1920

aaaggcaucc aucagaucau uggggcagcc uucaaguccu uguuuggagg caugucuugg     1980

uucucgcaaa uccugauugg uacucuccug auguggcuug ggcugaauac caagaacgga     2040

agcauauccu ugaugugucu cgcucugggc gguguguuaa ucuuucuguc aacggccgug     2100

aguggcgggg uuggaugcuc ugucgacuuc agcaaa                               2136


<210>  1788
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long CDS opt

<400>  1788
agaaucauua acgccaggaa ggagaaaaag cggcgcggca ccgacacaag cguggggaua       60

gucggacugc uccuuacuac ggcuauggca gccgaaguua cccgacgugg uuccgcguac      120

uauauguacc uggauagauc ugacgcuggc gaggccaucu cauuccccac aacuuugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc aaccaugagu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

acaaccagca cuuggguggu cuaugguaca ugucaucaca agaaagggga ggccaggcgg      360

ucccgcagag cugugacccu accgucucau ucgacgagga agcuccaaac ucggucacag      420

accuggcugg agagccgcga auacacaaag caccuuauuc gaguugagaa uuggaucuuu      480

agaaaccccg gauucgcacu ggccgcugcc gcgauagcau gguugcucgg caguuccacu      540

ucucagaaag ugaucuaucu ggucaugauu cugcucaucg ccccugcuua cagcauuagg      600

ugcaucggcg uguccaaucg ggauuuugua gaagggaugu caggagguac cuggguugac      660

guggucuuag agcauggcgg gugugugaca gugauggccc aggauaagcc aaccgucgac      720

auagagcugg uuacgacuac agugaguaac auggcagaag uacguagcua cugcuaugag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccca cccaaggaga agcguaccuu      840

gacaaacaga gugauacuca guaugugugc aagagaacau uggucgacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcucu cuagugaccu gcgccaaguu ugcaugcucc      960

aagaaaauga cggggaaguc uauccagccu gagaaccucg aauaccggau uaugcugucg     1020

guucacggau cucaacacuc cgguaugauc gugaaugaua ccggccauga gacagacgag     1080

aaccgagcua aagucgaaau cacuccaaau ucaccccgcg ccgaggcuac ccuuggggga     1140

uucggcaguc uggguuugga uugugaaccu agaacagggc ucgacuuuag cgaucuguau     1200

uacuuaacua ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc agauaccgga acaccccauu ggaacaauaa ggaagcccuu     1320

guagaguuua aagacgcuca cgcgaagagg cagaccguug ugguccuggg cucccaggag     1380

ggggcagugc auacugccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

cggcugucua gcgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucauaua guuuguguac ggcugccuuc acauuuucca aaauaccugc agaaacccuc     1560

cauggcacug ugaccgagga aguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gcagguuaau cacagcaaac     1680

ccagugauua cggagagcac cgagaacucu aagaugaugc uggaacuaga ucccccguuc     1740

ggagacucau acaucguuau aggcgugggg gagaaaaaga uuacucacca cuggcaucgu     1800

aguggaagca caaucgguaa agccuuugaa gcuaccgucc ggggcgccaa gcgaauggca     1860

gugcucgggg acacagcuug ggauuucgga uccguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagauuuu cggagcggcc uuuaagucgu uguucggcgg gaugagcugg     1980

uuuucccaaa uccugauugg aacucuccug auguggcuug gccugaacac caagaauggu     2040

ucaauaaguu ugaugugucu cgcuuuaggg ggagugcuga ucuuccuguc uacggcaguu     2100

agcgccgacg ucggcugcuc cguggauuuu ucaaaa                               2136


<210>  1789
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long CDS opt

<400>  1789
agaaucauua acgccaggaa ggagaaaaag cggcgcggca ccgacacaag cguggggaua       60

gucggacugc uccuuacuac ggcuauggca gccgaaguua cccgacgugg uuccgcguac      120

uauauguacc uggauagauc ugacgcuggc gaggccaucu cauuccccac aacuuugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc aaccaugagu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

acaaccagca cuuggguggu cuaugguaca ugucaucaca agaaagggga ggccaggcgg      360

ucccgcagag cugugacccu accgucucau ucgacgagga agcuccaaac ucggucacag      420

accuggcugg agagccgcga auacacaaag caccuuauuc gaguugagaa uuggaucuuu      480

agaaaccccg gauucgcacu ggccgcugcc gcgauagcau gguugcucgg caguuccacu      540

ucucagaaag ugaucuaucu ggucaugauu cugcucaucg ccccugcuua cagcauuagg      600

ugcaucggcg uguccaaucg ggauuuugua gaagggaugu caggagguac cuggguugac      660

guggucuuag agcauggcgg gugugugaca gugauggccc aggauaagcc aaccgucgac      720

auagagcugg uuacgacuac agugaguaac auggcagaag uacguagcua cugcuaugag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccca cccaaggaga agcguaccuu      840

gacaaacaga gugauacuca guaugugugc aagagaacau uggucgacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcucu cuagugaccu gcgccaaguu ugcaugcucc      960

aagaaaauga cggggaaaag cauccagccu gagaaccucg aauaccggau uaugcugucg     1020

guucacggau cucaacacuc cgguaugauc gugaaugaua ccggccauga gacagacgag     1080

aaccgagcua aagucgaaau cacuccaaau ucaccccgcg ccgaggcuac ccuuggggga     1140

uucggcaguc uggguuugga uugugaaccu agaacagggc ucgacuuuag cgaucuguau     1200

uacuuaacua ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc agauaccgga acaccccauu ggaacaauaa ggaagcccuu     1320

guagaguuua aagacgcuca cgcgaagagg cagaccguug ugguccuggg cucccaggag     1380

ggggcagugc auacugccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

cggcugucua gcgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucauaua guuuguguac ggcugccuuc acauuuacca aaauaccugc agaaacucuc     1560

cauggcaccg ugacaguuga ggugcaguac gcggguaccg acgggccaug caagguaccc     1620

gcucaaaugg ccgucgauau gcagacucug acaccugugg gcagguuaau cacggcaaac     1680

ccaguuauua ccgaauccac ugagaacagc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacucuu acaucgugau aggcgucggg gaaaagaaga uuacacacca cuggcaucgu     1800

ucaggaagua ccaucgguaa agccuuugag gcuacagugc ggggcgccaa gcgaauggca     1860

guacucgggg acacugcuug ggauuucgga agcgugggcg gugcccuuaa uucccugggg     1920

aaaggcaucc accagauuuu cggagcggcc uuuaagucuu uguucggcgg gaugucgugg     1980

uuuagccaaa uccugauugg aacccuccug auguggcuug gccugaacac gaagaauggu     2040

uccauaucau ugaugugucu cgcuuuaggg ggaguucuga ucuuccugag uaccgcaguc     2100

ucugccgacg ugggcugcag cguggauuuu uccaaa                               2136


<210>  1790
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long CDS opt

<400>  1790
agaaucauua acgccaggaa ggagaaaaag cggcgcggca ccgacacaag cguggggaua       60

gucggacugc uccuuacuac ggcuauggca guugaaguga cccgacgugg uaaugccuac      120

uauauguacc uggauagauc cgacgcgggc gaggcuaucu cuuuccccac aacuaugggg      180

augaacaaau gcuauauuca gaucauggau uugggacaca ugugugacgc caccauguca      240

uacgaaugcc cuauguuaga ugagggcgua gaaccagacg auguggacug uuggugcaau      300

acaaccagua cuugggucgu guaugguaca ugucaucaca agaaagggga ggcaaggcgg      360

agccgcagag ccguuacccu gccgucccau ucuacgagga agcuacaaac ucggucgcag      420

accuggcucg agucacgcga auacacaaag caccugauuc gaguggagaa cuggaucuuu      480

agaaaucccg gauucgcucu ugcagccgcu gccauagcgu ggcuguuggg cagcaguacu      540

ucccagaaag ucaucuaucu cgugaugauu cugcugaucg cacccgccua cucuauuagg      600

ugcaucggcg uaagcaaccg ggauuuuguu gaagggaugu ccggagguac cuggguggac      660

gucgugcucg agcauggcgg gugugugaca gucauggcuc aggauaagcc aaccguugac      720

auagaguuag ugacgacuac aguaucaaau auggccgaag ugcguaguua cugcuaugag      780

gcaagcauuu cugauauggc uuccgacuca cgcuguccca cccaaggaga agccuaccug      840

gacaaacaga gcgauacuca guaugucugc aagagaacac uuguggacag gggcuggggu      900

aacgggugug gauuguucgg caaaggcagu cugguuaccu gcgcgaaguu ugccugcucu      960

aagaaaauga cggggaaguc cauccagccu gagaaucuag aauaccggau uaugcucagc     1020

gugcacggau cgcaacacuc ugguaugauc gucaacgaua ccggccauga gacagacgag     1080

aaucgagcaa aaguggaaau cacuccaaac uccccucgcg cugaggccac ccuuggggga     1140

uucggcucac ugggucugga uugugaaccu agaacagggu uggacuuuag ugaucucuau     1200

uaccugacua ugaauaacaa gcacugguua guacauaaag agugguucca cgacauucca     1260

cugccguggc acgcuggcgc cgauaccgga acaccccauu ggaauaacaa ggaagcacuc     1320

guugaguuua aagacgccca cgcuaagagg cagaccgugg ucgugcuugg cagccaggag     1380

ggggcggucc auacugcacu ggccggagcu uuggaagccg agauggaugg ugcaaagggc     1440

cggcuauccu cugggcaccu gaaaugccgc cucaagaugg acaaacugag acuuaaggga     1500

gugagcuauu cacuguguac ggccgcuuuc acauuuacca aaauaccugc cgaaacuuug     1560

cauggcaccg uuacagugga gguacaguac gcagguaccg acgggccaug caaggucccc     1620

gcgcaaaugg cuguggauau gcagacucuc acaccuguug gcaggcugau cacggccaac     1680

ccagugauua ccgaaaguac ugaaaauucc aagaugaugu uagagcugga ucccccguuc     1740

ggagacagcu acaucgucau aggcgugggg gagaaaaaga uuacacacca cuggcaucgu     1800

ucuggaucaa ccaucgguaa agcauuugag gccacaguac ggggcgcuaa gcgaauggcc     1860

gugcuagggg acacugcaug ggauuucgga aguguuggcg gugcucucaa cagccugggg     1920

aaaggcaucc accagauuuu cggagccgcg uuuaaguccc uuuucggcgg gaugucuugg     1980

uuuucgcaaa ucuugauugg aacccugcuc gucuggcugg gccuuaauac gaagaacggu     2040

agcauauccc ugauguguuu ggcccucggg ggagugcuga ucuucuuauc aaccgcugug     2100

agugcagacg ucggcugcuc uguugauuuu agcaaa                               2136


<210>  1791
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long CDS opt

<400>  1791
agaaucauua acgccaggaa ggagaaaaag cggcgcggca ccgacacaag cguggggaua       60

gucggacugc uccuuacuac ggcuauggca gccgaaguua cccgacgugg uuccgcguac      120

uauauguacc uggauagauc ugacgcuggc gaggccaucu cauuccccac aacuuugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc aaccaugagu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

acaaccagca cuuggguggu cuaugguaca ugucaucaca agaaagggga ggccaggcgg      360

ucccgcagag cugugacccu accgucucau ucgacgagga agcuccaaac ucggucacag      420

accuggcugg agagccgcga auacacaaag caccuuauuc gaguugagaa uuggaucuuu      480

agaaaccccg gauucgcacu ggccgcugcc gcgauagcau gguugcucgg caguuccacu      540

ucucagaaag ugaucuaucu ggucaugauu cugcucaucg ccccugcuua cagcauuagg      600

ugcaucggcg uguccaaucg ggauuuugua gaagggaugu caggagguac cuggguugac      660

guggucuuag agcauggcgg gugugugaca gugauggccc aggauaagcc aaccgucgac      720

auagagcugg uuacgacuac agugaguaac auggcagaag uacguagcua cugcuaugag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccca cccaaggaga agcguaccuu      840

gacaaacaga gugauacuca guaugugugc aagagaacau uggucgacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcucu cuagugaccu gcgccaaguu ugcaugcucc      960

aagaaaauga cggggaaaag cauccagccu gagaaccucg aauaccggau uaugcugucg     1020

guucacggau cucaacacuc cgguaugauc gugaaugaua ccggccauga gacagacgag     1080

aaccgagcua aagucgaaau cacuccaaau ucaccccgcg ccgaggcuac ccuuggggga     1140

uucggcaguc uggguuugga uugugaaccu agaacagggc ucgacuuuag cgaucuguau     1200

uacuuaacua ugaacaauaa gcacuggcug gugcauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc agauaccgga acaccccauu ggaacaauaa ggaagcccuu     1320

guagaguuua aagacgcuca cgcgaagagg cagaccguug ugguccuggg cucccaggag     1380

ggggcagugc auacugccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

cggcugucua gcgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gucucauaua guuuguguac ggcugccuuc acauuuacca aaauaccugc agaaacucuc     1560

cauggcaccg ugacaguuga ggugcaguac gcggguaccg acgggccaug caagguaccc     1620

gcucaaaugg ccgucgauau gcagacucug acaccugugg gcagguuaau cacggcaaac     1680

ccaguuauua ccgaauccac ugagaacagc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacucuu acaucgugau aggcgucggg gaaaagaaga uuacacacca cuggcaucgu     1800

ucaggaagua ccaucgguaa agccuuugag gcuacagugc ggggcgccaa gcgaauggca     1860

guacucgggg acacugcuug ggauuucgga agcgugggcg gugcccuuaa uucccugggg     1920

aaaggcaucc accagauuuu cggagcggcc uuuaagucuu uguucggcgg gaugucgugg     1980

uuuagccaaa uccugauugg aacccuccug auguggcuug gccugaacac gaagaauggu     2040

uccauaucau ugguuugucu cgcuuuaggg ggaguccuga ucuuccugag uaccgcagug     2100

ucugccgacg ugggcugcag cgucgauuuu uccaaa                               2136


<210>  1792
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long CDS opt

<400>  1792
agaaucauua acgccaggaa ggagaaaaag cggcgcggca ccgacacaag cguggggaua       60

gucggacugc uccuuacuac ggcuauggca guugaaguga cccgacgugg uuccgccuac      120

uauauguacc uggauagauc ugacgcgggc gaggcuaucu cauuccccac aacuuugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc caccaugagu      240

uacgaaugcc cuaugcugga ugagggcgua gaaccagacg auguggacug uuggugcaac      300

acaaccagca cuugggucgu guaugguaca ugucaucaca agaaagggga ggcaaggcgg      360

ucccgcagag ccguuacccu accgucucau ucgacgagga agcuccaaac ucggucacag      420

accuggcugg agagccgcga auacacaaag caccuuauuc gaguggagaa uuggaucuuu      480

agaaaccccg gauucgcucu ggcagccgcu gccauagcgu gguugcucgg caguuccacu      540

ucucagaaag ucaucuaucu ggugaugauu cugcucaucg ccccugcaua cagcauuagg      600

ugcaucggcg uauccaaucg ggauuuuguu gaagggaugu caggagguac cuggguggac      660

gucguguuag agcauggcgg gugugugaca gucauggcuc aggauaagcc agccguugac      720

auagagcugg ugaccacgac uguaaguaac auggcagaag ugcguagcua cugcuaugag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccca cacaaggaga agcguaccuu      840

gacaaacaga gugauaccca guaugucugc aagagaacuu ugguggacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcucu cuaguuacau gcgccaaguu ugcaugcucc      960

aagaaaauga ccgggaaaag cauccagccu gagaaccucg aauaccggau uaugcugucg     1020

gugcacggau cucaacacuc cgguaugauc gucaaugaua cgggccauga gaccgacgag     1080

aaccgagcua aaguggaaau cacaccaaau ucaccccgcg ccgaggcuac ucuuggggga     1140

uucggcaguc uggguuugga uugugaaccu agaaccgggc ucgacuuuag cgaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guacauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guugaguuua aagacgcuca cgcgaagagg cagaccgugg ucgugcuggg cucccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

cggcugucua gcgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

gugucauaua guuuguguac ugcugccuuc acguuuacaa aaauaccugc agaaacccuc     1560

cauggcacug uuaccgugga gguacaguac gcggguacag acgggccaug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acuccuguug gcagguuaau cacagcaaac     1680

ccagugauua cggaauccac cgagaacagc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacucuu acaucgucau aggcgugggg gaaaagaaga uuacucacca cuggcaucgu     1800

ucaggaagua caaucgguaa agccuuugag gcuaccguac ggggcgccaa gcgaauggca     1860

gugcucgggg acacagcuug ggauuucgga agcguuggcg gugcccuuaa uucccugggg     1920

aaaggcaucc accagauuuu cggagcggcc uuuaagucuu uguucggcgg gaugucgugg     1980

uuuagccaaa uccugauugg aacucuccug gucuggcuug gccugaacac caagaauggu     2040

uccauaucau ugacgugucu cgcuuuaggg ggagugcuga ucuuccugag uaccgcagug     2100

ucugccgacg ucggcugcag cguugauuuu uccaaa                               2136


<210>  1793
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long CDS opt

<400>  1793
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc guugaaguga cgcgacgugg uuccgcguac      120

uauauguacc uggauagauc ugacgcuggc gaggccaucu cauuccccac cacauugggg      180

augaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc aacuaugagu      240

uacgaaugcc cuaugcugga ugagggcgua gaaccagacg auguggacug uuggugcaac      300

accacaagca ccugggucgu guaugguacu ugucaucaca agaaagggga ggccaggcgg      360

ucccgcagag cuguuacacu accgucucau ucgaccagga agcuccaaac gcggucacag      420

acuuggcugg agagccgcga auacaccaag caccuuauuc gaguggagaa uuggaucuuu      480

agaaaccccg gauucgcacu ggccgcugcc gucauagcgu gguugcucgg caguuccaca      540

ucucagaaag ugaucuaucu gguaaugauu cugcucaucg ccccugcaua cagcauuagg      600

ugcaucggcg uuuccaaucg ggauuuugug gaagggaugu caggagguac uugggucgac      660

gugguguuag agcauggcgg gugugucacc guuauggcuc aggauaagcc aacaguggac      720

auagagcugg uaaccacgac ugugaguaac auggccgaag uccguagcua cugcuaugag      780

gcaucuauuu ccgauauggc uucagacagc cgcuguccca cacaaggaga agccuaccuu      840

gacaaacaga gugauaccca guaugugugc aagagaacuu ugguugacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcucu cuagugacau gcgcgaaguu ugccugcucc      960

aagaaaauga ccgggaaaag cauccagccu gagaaccucg aauaccggau uaugcugucg     1020

guccacggau cucaacacuc cgguaugauc gugaaugaua cgggccauga gaccgacgag     1080

aaccgagcaa aaguagaaau cacaccaaau ucaccccgcg cugaggccac ucuuggggga     1140

uucggcaguc uggguuugga uugugaaccu agaaccgggc ucgacuuuag cgaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guucauaaag agugguucca cgacauucca     1260

cucccguggc acgcuggcgc cgauacugga acaccccauu ggaacaauaa ggaagcacuu     1320

guggaguuua aagacgccca cgcuaagagg cagaccgucg ugguccuggg cucccaggag     1380

ggggcggugc auaccgcauu ggccggagcu cuagaagccg agauggaugg ugcaaagggc     1440

cggcugucua gcgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

guuucauaua guuuguguac ugccgcuuuc acguuuacaa aaauaccugc cgaaacccuc     1560

cauggcacug ugaccguaga gguccaguac gcagguacag acgggccaug caaggugccc     1620

gcgcaaaugg cuguugauau gcagacccug acuccugugg gcagguuaau cacagccaac     1680

ccagucauua cggaauccac cgagaacagc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacucuu acaucgugau aggcguaggg gaaaagaaga uuacucacca cuggcaucgu     1800

ucaggaagua caaucgguaa agcauuugag gccaccgugc ggggcgcuaa gcgaauggcc     1860

guucucgggg acacagcaug ggauuucgga agcgucggcg gugcucuuaa uucccugggg     1920

aaaggcaucc accagauuuu cggagccgcg uuuaagucuu uguucggcgg gaugucgugg     1980

uuuagccaaa uccugauugg aacucuccug guguggcuug gccugaacac caagaauggu     2040

uccauaucau ugacgugucu cgccuuaggg ggagugcuga ucuuccugag uaccgcuguc     2100

ucugcagacg uuggcugcag cguggauuuu uccaaa                               2136


<210>  1794
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long CDS opt

<400>  1794
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc guugaaguga cgcgacgugg uuccgcguac      120

uauauguacc uggauagauc ugacgcuggc gaggccaucu cauuccccac cacauugggg      180

guaaauaaau gcuauauuca gaucauggau uuaggacaca ugugugacgc aacuaugagu      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg augucgacug uuggugcaac      300

accacaagca ccuggguggu uuaugguacu ugucaucaca agaaagggga ggccaggcgg      360

ucccgcagag cugugacacu accgucucau ucgaccagga agcuccaaac gcggucacag      420

acuuggcugg agagccgcga auacaccaag caccuuauuc gagucgagaa uuggaucuuu      480

agaaaccccg gauucgcacu ggccgcugcc gcgauagcau gguugcucgg caguuccaca      540

ucucagaaag ugaucuaucu gguaaugauu cugcucaucg ccccugcuua cagcauuagg      600

ugcaucggcg uuuccaaucg ggauuuugug gaagggaugu caggagguac uugggucgac      660

gugguguuag agcauggcgg gugugucacc guuauggccc aggauaagcc aacaguggac      720

auagagcugg uaaccacgac ugugaguaac auggcagaag uccguagcua cugcuaugag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccca cacaaggaga agcguaccuu      840

gacaaacaga gugauaccca guaugugugc aagagaacuu ugguugacag gggcuggggu      900

aaugggugug gacuguucgg caaaggcucu cuagugacau gcgccaaguu ugcaugcucc      960

aagaaaauga ccgggaaguc cauccagccu gagaaccucg aauaccggau uaugcugucg     1020

guccacggau cucaacacuc cgguaugauc gugaaugaua cgggccauga gaccgacgag     1080

aaccgagcua aaguagaaau cacaccaaau ucaccccgcg ccgaggcuac ucuuggggga     1140

uucggcaguc uggguuugga uugugaaccu agaaccgggc ucgacuuuag cgaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guucauaaag agugguucca cgacauucca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagagg cagaccgucg ugguccuggg cucccaggag     1380

ggggcagugc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

cggcugucua gcgggcaccu caaaugccgc cugaagaugg acaaacuuag acugaaggga     1500

guuucauaua guuuguguac ugcugccuuc acguuuacaa aaauaccugc agaaacccuc     1560

cauggcacug ugaccguaga gguccaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguugauau gcagacccug acuccugugg gcagguuaau cacagcaaac     1680

ccagucauua cggaauccac cgagaacagc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacucuu acaucgugau aggcguaggg gacaaaaaga uuacucacca cugguaucgu     1800

ucaggaagua caaucgguaa agccuuugaa gcuaccgugc ggggcgccaa gcgaauggca     1860

guucucgggg auacagcuug ggacuucgga agcgucggcg gugcccugaa uagccuuggg     1920

aaaggcaucc aucagauuuu cggagcggcc uuuaagucuu uguucggcgg gaugucgugg     1980

uuuagccaaa uccugauugg aacucuccug guguggcuug gccugaacac caagaauggu     2040

uccauaucau ugacgugucu cgcuuuaggg ggagugcuga ucuuccugag uaccgcaguc     2100

ucugccgaug uuggcugcag cguggacuuu uccaaa                               2136


<210>  1795
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long CDS opt

<400>  1795
agaaucauua acgccaggaa ggagaaaaag cggcgcggcg cugacaccag cguggggaua       60

gucggacugc uccuuacaac ugcaauggcc guugaaguga cgcgacgugg uuccgcguac      120

uauauguacc uggauagauc ugacgcuggc aaagccaucu cauuccccac cacauugggg      180

guaaauaagu gcuauauuca gaucauggau uuaggacaca ugugugacgc aacuaugagu      240

uacgagugcc cuaugcugga ugaaggcgug gagccagacg augucgacug uuggugcaac      300

accacaagca ccuggguggu uuaugguacu ugucaucaca aaaaggggga agccaggcgg      360

ucccgcagag cugugacacu accgucucau ucgaccagga agcuccaaac gcggucacag      420

acuuggcugg agagccgcga guacaccaaa caccuuauuc gagucgaaaa uuggaucuuu      480

agaaaccccg gauucgcacu ggccgcugcc gcgauagcau gguugcucgg caguuccaca      540

ucucagaagg ugaucuaucu gguaaugauu cugcucaucg ccccugcuua cagcauuagg      600

ugcaucggcg uuuccaaucg ggauuuugug gaggggaugu caggagguac uugggucgac      660

gugguguuag aacauggcgg gugugucacc guuauggccc aggauaaacc aacaguggac      720

auagagcugg uaaccacgac ugugaguaac auggcagagg uccguagcua cugcuaugag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccca cacaaggaga agcguaccuu      840

gacaagcaga gugauaccca guaugugugc aaaagaacuu ugguugacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuagugacau gcgccaaguu ugcaugcucc      960

aaaaagauga ccgggaaaag cauccagccu gaaaaccucg aguaccggau uaugcugucg     1020

guccacggau cucaacacuc cgguaugauc gugaaugaua ucggccauga aacggacgag     1080

aaccgagcua agguagagau uaccccaaau ucaccccgcg ccgaagcuac acuuggggga     1140

uucggcaguc uggguuugga uugugagccu agaacugggc ucgacuuuag cgaucuguau     1200

uacuuaacca ugaacaauaa acacuggcug guucauaagg aaugguucca cgacauacca     1260

cucccguggc acgccggcgc agauacagga acuccccauu ggaacaauaa agaggcccuu     1320

guggaauuua aggacgcuca cgcgaagagg cagaccgucg ugguccuggg cucccaggag     1380

ggggcagugc auacagccuu ggcuggagcc cuagaggcag aaauggaugg ugccaaaggc     1440

cggcugucua gcgggcaccu caagugccgc cugaaaaugg acaagcugag acugaaagga     1500

guuucauaua guuuguguac cgcugccuuc acuuuuacga agaucccugc agagacacuc     1560

cauggcaccg ugacuguaga aguccaguac gcggguaccg acgggccaug caaggugccc     1620

gcucaaaugg ccguugauau gcagacacug accccugugg gcagguuaau uacugcaaac     1680

ccagucauca cagaguccac ggaaaacagc aaaaugaugc uggagcuaga ucccccguuc     1740

ggagacucuu acauagugau uggcguaggg gacaagaaaa ucacccacca cuggcaucgu     1800

ucaggaagua cuaucgguaa ggccuuugag gcuacagugc ggggcgccaa acgaauggca     1860

guucucgggg auaccgcuug ggacuucgga agcgucggcg gugcccuuaa uucccuuggg     1920

aagggcauuc accagaucuu cggagcggcc uuuaagucuu uguucggcgg gaugucgugg     1980

uuuagccaaa uucugauagg aacacuccug guguggcuug gccugaacac uaaaaauggu     2040

uccaucucau ugaccugucu cgcuuuaggg ggagugcuga uuuuccugag uacggcaguc     2100

ucugccgaug uuggcugcag cguggacuuu uccaag                               2136


<210>  1796
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long CDS opt

<400>  1796
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

gugggacugc uccuuacaac ugcaauggcc gcggaaauua cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaaucu cauucgccac cacauugggg      180

gucaauaaau gccacguuca gauuauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcgug gagccugacg auguagacug uuggugcaac      300

accacaagca ccuggguggu cuacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgugacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauca aaguugaaaa uuggauauuu      480

agaaacccgg gauucacacu gguggcuguc gccaucgcgu gguugcucgg caguuccacu      540

ucucagaagg ugauuuaccu gguaaugauc cugcucauug cacccgccua uagcaucagg      600

ugcauaggcg uuuccaaucg ggauuuugug gaggggaugu caggagguac cugggucgac      660

gugguguuag aacacggcgg gugugucaca guuauggcuc aggauaaacc uaccguggac      720

auugagcugg uaacgacuac agugaguaac auggccgagg uccguagcua cugcuacgaa      780

gcaucuaucu ccgauauggc uucagacagc cgcuguccaa cccaaggaga ggccuaucuu      840

gacaagcaga gugauacuca guacgugugc aaaagaacau ugguugacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuagugaccu gcgcgaaguu uacgugcucc      960

aaaaagauga ccgggaaaag cauucagccc gaaaaccucg aguaucggau caugcugucg     1020

guccacggau cucaacauuc cgguaugauc gugaaugaua caggccacga aacugacgag     1080

aaccgagcca agguagaggu uaccccuaau ucaccacgcg cagaagcuac acuuggggga     1140

uucggcaguc uggguuugga uugugagccc agaacugggc ucgacuuuag cgaucuguac     1200

uauuuaacca ugaacaauaa acauuggcug gugcacaagg aaugguucca cgacauuccu     1260

cucccauggc augccggcgc ugauacagga accccgcacu ggaacaauaa agaggcccuu     1320

gucgaauuua aggacgcaca ugccaagagg cagacugugg ucgugcuggg cucccaggag     1380

ggggcuguuc acacggcguu ggcaggagcc cuagaggcug aaauggaugg ugccaaaggc     1440

cggcuguucu cugggcaucu caagugccgc cugaaaaugg acaaacuuag acugaaggga     1500

gugagcuacu cauuguguac agcagccuuu accuucacua agguacccgc ugagacccuc     1560

cacggcacag ucaccgugga aguucaguau gccgguacug acgggccuug caaggugcca     1620

gcacaaaugg cggucgauau gcagacacug acgcccgugg gcagguuaau aaccgcuaac     1680

ccuguaauca cugagaguac agaaaacucc aaaaugaugc uggagcuaga cccacccuuu     1740

ggagauagcu acauugugau cggcguuggg gacaagaaaa uaacccacca uuggcaccgu     1800

ucuggaucaa caauugguaa ggccuucgag gcaacugucc ggggcgccaa acgaauggcu     1860

gugcucgggg auaccgccug ggacuucgga agugugggcg gugucuuuaa uagccugggg     1920

aagggcaucc aucagaucuu cggagcagcu uuuaaguccc uuuucggcgg gaugucuugg     1980

uuuucgcaaa uuuugaucgg aacgcugcuc guuuggcugg gccuuaacac caaaaauggu     2040

agcauuuccc ugacauguuu ggcccucggg ggagugauga uauuccuguc aacugcggug     2100

agugccgaug uaggcugcuc ugucgacuuu agcaag                               2136


<210>  1797
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long CDS opt

<400>  1797
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac caauuugggg      180

gugaacaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacaaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaau      300

acuaccagca cauggguagu guacgguacc ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgucacucu accaucucac ucgacacgga agcuccaaac ccgcucacag      420

acguggcugg agagccgaga guauacuaag caucuuauaa aaguggaaaa cuggaucuuu      480

agaaauccgg gauucgcucu ggccgcgguu gcaauugccu gguugcucgg caguuccacc      540

ucucagaagg ugaucuaccu ggucaugauu cugcucaucg cucccgccua uagcauaagg      600

ugcauuggcg uguccaaccg ggauuuugua gaggggaugu caggagguac auggguugac      660

guggucuuag aacacggcgg gugugugacu gugauggcac aggauaaacc uaccgucgac      720

aucgagcugg uuacaaccac ggugaguaau auggcugagg uacguagcua cugcuacgaa      780

gccucuauuu ccgauauggc gucagacagc cgcuguccaa cucaaggaga ggccuaucuu      840

gacaagcaga gugauacaca guacgugugc aaaagaaccu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caagggcucu cuagugacuu gcgcaaaguu uacaugcucc      960

aaaaagauga ccgggaaaag cauccagccc gaaaaucucg aguaucggau caugcugucg     1020

guucacggau cucaacauuc cgguaugauu gugaacgaua uaggccacga aacggacgag     1080

aaucgagcua aggucgaggu gaccccuaac ucaccacgcg ccgaagcuac acuuggggga     1140

uucggcaguc uggguuugga uugugagccc agaacugggc ucgacuuuag cgaucuguac     1200

uauuuaacca ugaauaacaa acauuggcug guacacaagg aaugguucca cgacaucccu     1260

cucccauggc augccggcgc agauacagga acuccgcacu ggaauaacaa agaggcccuu     1320

guugaauuua aggacgcuca ugcgaagagg cagaccgugg ucgugcuggg cucccaggag     1380

ggggcagucc acacagccuu ggcuggagcc cuagaggcag aaauggaugg ugccaaaggc     1440

cggcuguucu cugggcaucu caagugccgc cugaaaaugg acaaacuuag acugaaggga     1500

gugagcuacu cauuguguac cgcugccuuu acuuucacga agguucccgc agagacacuc     1560

cacggcaccg ugacuguaga aguccaguau gcggguaccg acgggccuug caaggugcca     1620

gcucaaaugg ccguugauau gcagacacug acccccgugg gcagguuaau uacugcaaac     1680

ccugucauca cagagaguac ggaaaauucc aaaaugaugc uggagcuaga cccacccuuu     1740

ggagauagcu acauagugau uggcguaggg gacaagaaaa ucacccacca uuggcaccgu     1800

ucuggaucaa cuaucgguaa ggccuucgag gcuacagugc ggggcgccaa acgaauggca     1860

guucucgggg auaccgcuug ggacuucgga agugucggcg guguguuuaa cagccugggg     1920

aagggcgugc aucagauuuu cggagccgcg uuuaaguccc uuuucggcgg gaugucuugg     1980

uuuucgcaaa ucuugauugg aacacugcuc gucuggcugg gccuuaauac uaaaaacggu     2040

agcauauccc ugaccuguuu ggcccucggg ggaguuauga ucuuccuguc aacggcugug     2100

agugcagaug ugggcugcuc uguagacuuu agcaag                               2136


<210>  1798
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long CDS opt

<400>  1798
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacauugggg      180

gugaauaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgucacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauaa aaguggaaaa uuggaucuuu      480

agaaacccgg gauucgcucu gguugccgug gcgauugcau gguugcucgg caguuccaca      540

ucucagaagg ucaucuaccu ggugaugauu cugcucaucg cccccgcuua uagcauaagg      600

ugcauuggcg uauccaaucg ggauuuuguu gaggggaugu caggagguac uuggguggac      660

gucguguuag aacacggcgg gugugugacc gucauggccc aggauaaacc uacaguugac      720

aucgagcugg ugaccacgac uguaaguaac auggcagagg ugcguagcua cugcuacgag      780

gcuucuauuu ccgauauggc cucagacagc cgcuguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgucugc aaaagaacuu ugguggacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuaguuacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauccagccc gaaaaccucg aguaucggau caugcugucg     1020

gugcacggau cucaacauuc cgguaugauu gucaaugaua uaggccacga aaccgacgag     1080

aaccgagcaa agguggaggu aacaccuaau ucaccacgcg cugaagccac ucuuggggga     1140

uucggcaguc uggguuugga uugugagccc agaaccgggc ucgacuuuag cgaucuguac     1200

uauuuaacaa ugaacaauaa acauuggcug guucacaagg aaugguucca cgacaucccu     1260

cucccauggc augcuggcgc cgauacugga accccgcacu ggaacaauaa agaggcacuu     1320

guggaauuua aggacgccca ugcuaagagg cagacagucg ugguccuggg cucccaggag     1380

ggggcggugc acaccgcauu ggccggagcu cuagaggccg aaauggaugg ugcaaaaggc     1440

cggcuguucu cugggcaucu caagugccgc cugaaaaugg acaaacuuag acugaaggga     1500

guuagcuacu cauuguguac ugccgcuuuu acguucacaa aggugcccgc cgagacccuc     1560

cacggcacug uaaccgucga agugcaguau gcagguacag acgggccuug caagguucca     1620

gcgcaaaugg cuguggauau gcagacccug acucccgucg gcagguuaau uacagccaac     1680

ccugugauca cggagaguac cgaaaacucc aaaaugaugc uggagcuaga cccacccuuu     1740

ggagauagcu acauaguaau uggcgugggg gacaagaaaa ucacucacca uuggcaccgu     1800

ucuggaucaa caaucgguaa ggcauucgag gccaccguuc ggggcgcuaa acgaauggcc     1860

guccucgggg auacagcaug ggacuucgga agugugggcg guguguuuaa uagccugggg     1920

aagggcauuc aucagaucuu cggagcugcc uuuaaguccc uuuucggcgg gaugucuugg     1980

uuuucgcaaa uuuugauagg aacucugcuc gucuggcugg gccuuaacac caaaaauggu     2040

agcaucuccc ugacguguuu ggcgcucggg ggaguuauga uuuuccuguc aaccgccgug     2100

agugcugaug ugggcugcuc uguagacuuu agcaag                               2136


<210>  1799
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long CDS opt

<400>  1799
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac caauuugggg      180

gugaacaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacaaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaau      300

acuaccagca cauggguagu guacgguacc ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgucacucu accaucucac ucgacacgga agcuccaaac ccgcucacag      420

acguggcugg agagccgaga guauacuaag caucuuauaa aaguggaaaa cuggaucuuu      480

agaaauccgg gauucgcucu ggccgcgguu gcaauugccu gguugcucgg caguuccacc      540

ucucagaagg ugaucuaccu ggucaugauu cugcucaucg cucccgccua uagcauaagg      600

ugcauuggcg uguccaaccg ggauuuugua gaggggaugu caggagguac auggguugac      660

guggucuuag aacacggcgg gugugugacu gugauggcac aggauaaacc uaccgucgac      720

aucgagcugg uuacaaccac ggugaguaau auggcugagg uacguagcua cugcuacgaa      780

gccucuauuu ccgauauggc gucagacagc cgcuguccaa cucaaggaga ggccuaucuu      840

gacaagcaga gugauacaca guacgugugc aaaagaaccu uggucgacag gggcuggggu      900

aacgggugug gacuguucgg caagggcucu cuagugacuu gcgcaaaguu uacaugcucc      960

aaaaagauga ccgggaaaag cauccagccc gaaaaucucg aguaucggau caugcugucg     1020

guucacggau cucaacauuc cgguaugauu gugaacgaug aaaaucgagc uaaggucgag     1080

gugacgccua acucaccacg cgccgaggcu acccuuggcg gguucggaag ucugggcuug     1140

gacugugaac ccagaacagg ucucgauuuu agcgaccugu acuauuuaac uaugaauaac     1200

aaacacuggc ugguacauaa ggagugguuc cacgauauac cucucccaug gcacgccggg     1260

gcagacaccg gcacaccgca uuggaauaac aaagaagccc uuguugaguu uaaggaugcu     1320

cacgcgaaga ggcagacugu ggucgugcug ggaucccagg aaggcgcagu ccauaccgcc     1380

uuggcugggg cccuagaggc agagauggac ggagccaaag gucggcuguu cucuggccac     1440

cucaagugcc gccugaaaau ggauaaacuu agacugaaag gggugagcua cucauugugu     1500

acagcugccu uuaccuucac uaagguuccc gcagaaacgc uccauggaac agugaccgua     1560

gagguccagu augcgggcac ugacgguccu ugcaaggugc cagcucaaau ggccguugac     1620

augcagaccc ugacacccgu ggggagguua aucaccgcaa acccugucau uacugaaagu     1680

acagaaaauu ccaaaaugau gcuggagcua gauccacccu uuggcgacag cuacaucgug     1740

auaggaguag gcgauaagaa aauuacgcac cacuggcauc guucuggguc aaccaucgga     1800

aaggccuucg aggcuacugu gcggggugcc aaacgaaugg caguucucgg cgacacagcu     1860

ugggauuucg ggagugucgg aggcguguuu aacagccugg guaaggggau ccaccagauu     1920

uucggcgccg cguuuaaguc ccuuuucgga ggcaugucuu gguuuucgca aaucuugauu     1980

gggacccugc ucguguggcu gggacuuaau acaaagaacg gcagcauauc ccugacuugu     2040

uuggcccucg guggggucau gaucuuccug ucaaccgcug uuagugcaga cgugggaugc     2100

ucuguggauu uuagcaag                                                   2118


<210>  1800
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long CDS opt

<400>  1800
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

gugggacugc uccuuacaac ugcaauggcc gcggaaauua cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaaucu cauucgccac cacauugggg      180

gucaauaaau gccacguuca gauuauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcgug gagccugacg auguagacug uuggugcaac      300

accacaagca ccuggguggu cuacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgugacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauca aaguugaaaa uuggauauuu      480

agaaacccgg gauucgcucu gguggccguc gcgaucgcau gguugcucgg caguuccaca      540

ucucagaagg ugauuuaccu gguaaugauc cugcucauug cccccgcuua uagcaucagg      600

ugcauaggcg uuuccaaucg ggauuuugug gaggggaugu caggagguac uugggucgac      660

gugguguuag aacacggcgg gugugucacc guuauggccc aggauaaacc uacaguggac      720

auugagcugg uaaccacgac ugugaguaac auggcagagg uccguagcua cugcuacgag      780

gcuucuaucu ccgauauggc cucagacagc cgcuguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgugugc aaaagaacuu ugguugacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuagugacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauucagccc gaaaaccucg aguaucggau caugcugucg     1020

guccacggau cucaacauuc cgguaugauc gugaaugaua ccggccacga aacagacgag     1080

aaccgagcaa agguagaggu uacuccuaau ucaccacgcg cugaagccac ccuuggggga     1140

uucggcaguc uggguuugga uugugagccc agaacagggc ucgacuuuag cgaucuguac     1200

uauuuaacua ugaacaauaa acauuggcug gugcacaagg aaugguucca cgacauuccu     1260

cucccauggc augcuggcgc cgauaccgga acaccgcacu ggaacaauaa agaggcacuu     1320

gucgaauuua aggacgccca ugcuaagagg cagaccgugg ucgugcuggg cucccaggag     1380

ggggcgguuc acacugcauu ggccggagcu cuagaggccg aaauggaugg ugcaaaaggc     1440

cggcuguucu cugggcaucu caagugccgc cugaaaaugg acaaacuuag acugaaggga     1500

gugagcuacu cauuguguac ggccgcuuuu acauucacca agguacccgc cgagacucuc     1560

cacggcaccg ucacagugga aguucaguau gcagguaccg acgggccuug caaggugcca     1620

gcgcaaaugg cugucgauau gcagacucug acacccgugg gcagguuaau aacggccaac     1680

ccuguaauca ccgagaguac ugaaaacucc aaaaugaugc uggagcuaga cccacccuuu     1740

ggagauagcu acauugugau cggcguuggg gacaagaaaa uaacacacca uuggcaccgu     1800

ucuggaucaa ccauugguaa ggcauucgag gccacagucc ggggcgcuaa acgaauggcc     1860

gugcucgggg auacugcaug ggacuucgga agugugggcg gugucuuuaa uagccugggg     1920

aagggcaucc aucagaucuu cggagcugcc uuuaaguccc uuuucggcgg gaugucuugg     1980

uuuucgcaaa uuuugaucgg aacccugcuc guuuggcugg gccuuaacac gaaaaauggu     2040

agcauuuccc ugaccuguuu ggcgcucggg ggagugauga uauuccuguc aacagccgug     2100

agugcugaug uaggcugcuc ugucgacuuu agcaag                               2136


<210>  1801
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long CDS opt

<400>  1801
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

gugggacugc uccuuacaac ugcaauggcc gcggaaauua cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaaucu cauucgccac cacauugggg      180

gucaauaaau gccacguuca gauuauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcgug gagccugacg auguagacug uuggugcaac      300

accacaagca ccuggguggu cuacgguacu ugucaacaca agaaagggga agcacggcgc      360

uccagaaggg ccgugacacu accaucucau ucgacccgga agcuccagac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caccuuauca aaguugaaaa uuggauauuu      480

agaaacccgg gauucgcucu gguggccguc gcgaucgcau gguugcucgg caguuccaca      540

ucucagaagg ugauuuaccu gguaaugauc cugcucauug cccccgcuua uagcaucagg      600

ugcauaggcg uuuccaaucg ggauuuugug gaggggaugu caggagguac uugggucgac      660

gugguguuag aacauggcgg gugugucacc guuauggccc aagauaaacc uacaguggac      720

auugagcugg uaaccacgac ugugaguaac auggcagagg uccguagcua cugcuacgag      780

gcuucuaucu ccgauauggc cucagacagc cgcuguccaa cacagggaga agcguaucuu      840

gacaagcaga gugauaccca guacgugugc aaaagaacuu ugguugacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuagugacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauucaaccc gaaaaccucg aguaucggau caugcugucg     1020

guccacggau cucagcacuc cgguaugauc gugaaugaua uuggccauga aaccgacgag     1080

aaccgagcaa agguagaggu uacaccuaau ucaccacgcg cugaagccac ucuuggggga     1140

uucggcaguc uggguuugga uugugagccc agaaccgggc ucgacuuuag cgaucuguac     1200

uauuuaacaa ugaacaauaa acacuggcug gugcauaagg aaugguucca cgacauaccu     1260

cucccauggc acgcuggcgc cgauacugga accccgcauu ggaacaauaa agaggcacuu     1320

gucgaauuua aggacgccca cgcuaagagg cagacagugg ucgugcuggg cucccaggag     1380

ggggcgguuc auaccgcauu ggccggagcu cuagaggccg aaauggaugg ugcaaaaggc     1440

cggcuguucu cugggcaccu caagugccgc cugaaaaugg acaaacuuag acugaaggga     1500

gugagcuacu cauuguguac ugccguaugc acggcugcca agguccccgc agagacacuc     1560

cauggcaccg ugacuguuga agugcaauau gcggguaccg acgggccuug uaagguccca     1620

gcucagaugg ccguggauau gcagacacug acccccguag gcagguuaau cacugcaaac     1680

ccugugauua cagagaguac ggaaaacucc aaaaugaugc uggagcuaga cccacccuuu     1740

ggagauagcu acaucguuau aggcgucggg gacaagaaaa uuacccacca cuggcaucgu     1800

ucuggaucaa cuaucgguaa ggccuucgag gcuacagugc ggggcgccaa acgaauggca     1860

gugcucgggg auaccgcuug ggacuuugga agugucggcg guguuuucaa uagccugggg     1920

aagggcaucc accaaauuuu cggagccgcg uuuaaguccc uuuucggcgg gaugucuugg     1980

uuuucgcaga ucuugauugg aacacugcuc guguggcugg gccuuaacac uaaaaauggu     2040

agcauauccc ugaccugcuu ggcccucggg ggagugauga ucuuccuguc aacggcugua     2100

agugcagaug ucggcuguuc uguggacuuu agcaag                               2136


<210>  1802
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long CDS opt

<400>  1802
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacgcaag cauagggauc       60

gugucccugc uccuuaccac agucauggcc gcggaaauua cucguagagg aucugcuuac      120

uauauguacc uggauagguc agacgccggu aaggcaauca guuucguuac gaccuugggc      180

gugaauaaau gccacguaca gauuauggau uuagggcaua ugugugacgc cacaaugagc      240

uaugagugcc ccaugcugga ugaaggagug gagccugacg augucgacug uuggugcaac      300

acuaccucca cauggguggu uuacggcacc ugucaccaua agaaagguga agcucggcgc      360

ucuagaaggg cagugacucu accaucgcac ucaacacgga agcuccaaac ccgcagccag      420

acguggcugg agagucgaga guauacuaag caucuuauca aagucgaaaa uuggauauuu      480

agaaacccgg gguucgcccu gguggcugua gccaucgcgu gguugcucgg auccucuacc      540

agccagaagg uuauuuaccu ggugaugauc cugcucauug cacccgccua uuccaucagg      600

ugcauaggcg ucucaaaucg ggauuuugug gagggcauga gugggggaac auggguggac      660

gucguuuuag aacacggugg cugugugacu guaauggcuc aggauaaacc uaccguggac      720

auugagcugg ucacaaccac ggugagcaac auggccgagg uucguucuua cugcuacgaa      780

gcauccaucu cagauauggc uagcgacagu cgcuguccaa cucaagggga ggccuaucuu      840

gacaagcagu cugauacaca guacgugugc aaaagaaccu uggucgacag gggauggggc      900

aaugguugug ggcuguucgg aaagggcucc cuagugacuu gcgcgaaguu uacaugcagc      960

aaaaagauga ccggcaaauc gauucagccc gaaaaccucg aguaucggau caugcugucu     1020

guacacgggu cccaacauuc aggaaugauc guuaaugaug aaaaccgagc caagguggag     1080

gucacgccua auaguccacg cgcagaggcu acccuuggug gcuucgggag ccugggauug     1140

gacugugaac ccagaacagg ccucgauuuu uccgaccugu acuauuuaac uaugaacaau     1200

aaacacuggc uggugcauaa ggagugguuc cacgauauuc cucucccaug gcacucuggu     1260

gccgacaccg aaacaccgca uuggaacaau aaagaggcuc uugucgaauu uaaggaugcc     1320

cacgcaaaga ggcagacugu gguugugcug gggagccagg agggcgccgu acauaccgcu     1380

uuggcgggag cacuagaggc cgaaauggac ggcgcuaaag ggcggcuguc aaguggacac     1440

cucaagugcc gccugaaaau ggauaaacuu agacugaaag gugucuccua cagcuugugu     1500

acagccgcau ucaccuuuac uaaggugccc gccgagacgc uccauggcac aguuaccgug     1560

gaaguccagu augcugggag ggacggaccu ugcaaggugc cagcccaaau ggcaguagac     1620

augcagacuc ugacccccgu gggccguuua auaacagcga acccuguuau caccgagucu     1680

acugaaaacu caaaaaugau gcuggagcua gauccacccu ucggugacag uuacauuguc     1740

aucggggugg gcgauaagaa aauaacacac cacuggcauc ggagcggauc cauuaucggc     1800

aaggcuuuug aggccacggu gcgaggggca aaaagaaugg ccguccucgg agacaccgcu     1860

ugggauuucg guucuguugg cgggguguuc aauucgcugg gaaagggcau ccaccagauu     1920

uuuggugccg cauucaagag ccuuuuuggg ggcauguccu gguucucaca aaucuugauu     1980

ggaacucugc ucguguggcu gggccuuaac acaaagaaug ggaguauauc ucugaccugu     2040

uuggcucucg gaggcguaau gaucuuucug agcacagccg ucuccgcgga cguggguugc     2100

ucaguugauu ucagcaag                                                   2118


<210>  1803
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long CDS opt

<400>  1803
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

gugggacugc uccuuacaac ugcaauggcc gcggaaauua cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaaucu cauucgccac cacauugggg      180

gucaauaaau gccacguuca gauuauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcgug gagccugacg auguagacug uuggugcaac      300

accacaagca ccuggguggu cuacgguacu ugucaccaua agaaagggga agcacggcgc      360

uccagaaggg ccgugacacu accaucucac ucgacccgga agcuccaaac gcgcucacag      420

acuuggcugg agagccgaga guauaccaag caucuuauca aaguugaaaa uuggauauuu      480

agaaacccgg gauucgcucu gguggccguc gcgaucgcau gguugcucgg caguuccaca      540

ucucagaagg ugauuuaccu gguaaugauc cugcucauug cccccgcuua uagcaucagg      600

ugcauaggcg uuuccaaucg ggauuuugug gaggggaugu caggagguac uugggucgac      660

gugguguuag aacacggcgg gugugucacc guuauggccc aggauaaacc uacaguggac      720

auugagcugg uaaccacgac ugugaguaac auggcagagg uccguagcua cugcuacgag      780

gcuucuaucu ccgauauggc cucagacagc cgcuguccaa cacaaggaga agcguaucuu      840

gacaagcaga gugauaccca guacgugugc aaaagaacuu ugguugacag gggcuggggu      900

aaugggugug gacuguucgg caagggcucu cuagugacau gcgccaaguu uaccugcucc      960

aaaaagauga cggggaaaag cauucagccc gaaaaccucg aguaucggau caugcugucg     1020

guccacggau cucaacauuc cgguaugauc gugaaugaug aaaaccgagc aaagguagag     1080

guuaccccua auucaccacg cgcugaggcc acacuuggcg gguucggaag ucugggcuug     1140

gacugugaac ccagaacugg ucucgauuuu agcgaccugu acuauuuaac caugaacaau     1200

aaacacuggc uggugcauaa ggagugguuc cacgauauuc cucucccaug gcacgcuggg     1260

gccgacacag gcacuccgca uuggaacaau aaagaagcac uugucgaguu uaaggaugcc     1320

cacgcuaaga ggcagaccgu ggucgugcug ggaucccagg aaggcgcggu ucauacagca     1380

uuggccgggg cucuagaggc cgagauggac ggagcaaaag gucggcuguu cucuggccac     1440

cucaagugcc gccugaaaau ggauaaacuu agacugaagg gggugagcua cucauugugu     1500

accgccgcuu uuacuuucac gaagguaccc gccgaaacac uccauggaac cgucacugug     1560

gagguucagu augcaggcac cgacgguccu ugcaaggugc cagcgcaaau ggcugucgac     1620

augcagacac ugacccccgu ggggagguua auaacugcca acccuguaau cacagaaagu     1680

acggagaacu ccaaaaugau gcuggaacua gauccacccu uuggcgacag cuacauugug     1740

aucggaguug gcgauaagaa aauaacccac cacuggcauc guucuggguc aacuauugga     1800

aaggcauucg aggccacagu ccggggugcu aaacgaaugg ccgugcucgg cgacaccgca     1860

ugggauuucg ggaguguggg aggcgucuuu aauagccugg guaaggggau ccaccagauc     1920

uucggcgcug ccuuuaaguc ccuuuucgga ggcaugucuu gguuuucgca aauuuugauc     1980

gggacacugc ucguuuggcu gggacuuaac acuaaaaaug gcagcauuuc ccugaccugu     2040

uuggcgcucg guggggugau gauauuccug ucaacggccg ugagugcuga cguaggaugc     2100

ucugucgauu uuagcaag                                                   2118


<210>  1804
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long CDS opt

<400>  1804
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacauugggg      180

gugaauaaau gccacgucca gaucauggau uuaggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccaugcugga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccaua agaaagggga aacacggcgc      360

uccagaaggu cugucucgcu acgguaucac uacacccgca agcuccaaac gcgaucacag      420

acuuggcugg agagcagaga guauaagaaa caucuuauaa ugguggaaaa uuggaucuuu      480

aggaacccag gauucgcaau uguuagugug gccaucaccu ggcugauggg cuccuugaca      540

ucucagaagg ucauuuaccu cgugaugauc guacugauag uuccggcuua cagcauuucc      600

ugcaucggcg ugucaaaucg ggaucugguc gaggggauga guggagguac uuggguggac      660

gugguccucg aacacggcgg guguguuacc gagauggccc aggauaaacc cacaguggac      720

auugaguuag uaaccaugac ggugagcaac auggcggaag uccguucuua uugcuacgag      780

gcaucccugu cagauauggc cagcgcuagu cgcuguccua cucaaggaga accaucucuu      840

gacaagcagu ccgacacaca gagcgugugc aaaagaaccu ugggcgauag ggguuggggg      900

aauggaugug gcaucuuugg caaggggucg cugguuacuu gcucuaaguu cacaugcugu      960

aaaaagaugc ccggaaaauc cauccagccu gagaaccuag aauaucggau uaugcuccca     1020

gugcacgguu cacaacauag uggcaugaua gucaaugaca ucgggcacga gaccgaugag     1080

aaccgagcca agguggaagu aacgcccaau agcccucgcg cagaggcuac ccugggaggc     1140

uuugguuccc uugggcugga cugcgaacca agaacaggcu uggauuucuc ugaccucuac     1200

uaucugacua ugaacaauaa acauugguua guucacaagg agugguuuca cgauauuccg     1260

cugcccuggc augccggagc ggacaccggc acaccucacu ggaacaauaa agaagcccuc     1320

guggaguuca aggaugcaca ugcuaagagg cagacugucg ugguccuugg gagccaggag     1380

ggagccgugc acaccgcucu ggccggugca uuggaagccg agauggacgg cgcuaaaggg     1440

cggcuauuuu caggacaucu gaagugucgc cucaaaaugg auaagcugag acuuaaaggc     1500

guuaguuacu cccugugcac agcggcauuc accuuuacua aggugccagc cgaaacguug     1560

cacgguacag uaaccgucga ggugcagagc gcugggacug acggccccug uaagguuccu     1620

gcccaaaugg caguggacau gcagacccuc acaccagucg gaaggcugau caccgccaac     1680

cccgugauaa cugaaucuac agagaacuca aaaaugaugu uagagcugga uccgccuuuc     1740

ggcgacaguu auauuguaau cgggguggga gauaagaaaa ucacgcacca uuggcaccgu     1800

agcgguucca ccauuggcaa ggcuuuugaa gccacuguuc gaggggcaaa acggauggcg     1860

guccuaggag acacagcuug ggauuucggc ucugugggug ggguguucaa uucgcucggc     1920

aagggaaucc aucagauuuu uggcgccgca uucaagagcc uguuuggggg aauguccugg     1980

uucucacaaa uacuuaucgg caccuugcug gucuggcucg gucugaacac uaaaaauggg     2040

aguauuucuc uuacaugccu ggccuuggga ggcguuauga ucuuucucag caccgcugug     2100

uccgccgacg uggguuguuc aguagauuuc agcaag                               2136


<210>  1805
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long CDS opt

<400>  1805
agaaucauua acgccaggaa ggagcggaaa cgccgaggcg cugacaccag cauagggauc       60

auuggacugc uccuuacaac ugcaauggcc gcggaaauca cgcguagagg uuccgcuuac      120

uauauguacc uggauagguc ugacgccggc aaggcaauuu cauucgccac cacaaugggg      180

gugaauaaau gccacgucca gaucauggau uugggacaua ugugugacgc uacuaugagu      240

uaugagugcc ccauguuaga ugaaggcguu gagccugacg auguggacug uuggugcaac      300

accacaagca ccuggguagu guacgguacu ugucaccaua agaaagggga agcacggcca      360

ucccgcagag ccgucacacu gccgucucac ucgaccagga agcuacaaac gcggucacag      420

acuuggcucg agagccgcga guauaccaag caucugauaa aaguggaaaa uuggauccuu      480

cgaaaccccg gauuuacagu ugcugcccug gcgauggcau gguugcucgg caauaguacu      540

ucccagagag ugauuuaccu ggucaugauc cugcucauug ccccugcuua uucuaucagg      600

ugcauaggcg ugagcaaccg ggauuucgua gaggggaugu ccggagguac cuggguugac      660

guggucuuag aacacggcgg gugugugaca gugauggccc aggauaagcc aaccgucgac      720

auugagcugg uuacgacuac agugucaaau auggcagagg uacguaguua cugcuacgaa      780

gcuagcaucu cugauauggc cuccgacuca cgcuguccca cccaaggaga ggcguaucuu      840

gacaaacaga gcgauacuca guacgugugc aagagaacau uggucgacag gggcuggggu      900

aacgggugug gacuguuugg caaaggcagu cuagugaccu gcgccaaguu cacgugcucu      960

aagaaaauga ccgggaaguc cauucagccu gaaaaucucg aguaucggau caugcugagc     1020

guucacggau cgcaacauuc ugguaugauc gugaacgaua uuggccacga aacagacgag     1080

aaucgagcaa aagucgaggu gacuccaaac ucaccccgcg cugaagccac ccuuggggga     1140

uuuggcuccc uggguuugga uugugagccu agaacagggc ucgacuucag ugaucuguac     1200

uauuuaacua ugaauaacaa gcauuggcug guacacaaag aaugguuuca cgacauacca     1260

cucccguggc augcuggcgc cgauaccgga acaccccacu ggaauaacaa ggaggcacuu     1320

guugaguuca aagacgccca ugcuaagagg cagaccgugg ucgugcuggg cagccaggaa     1380

ggggcggucc acacugcauu ggccggagcu cuagaggccg aaauggaugg ugcaaagggc     1440

cggcuguuuu ccgggcaucu caaaugccgc aucaagaugg acaaacugag acuuaaggga     1500

gugucuuaca gccuguguac ggccgcuuuc acauuuucaa aaguuccugc cgagaccuug     1560

cacggcacug ugaccguaga aguccaguau gcagguaggg acgggccaug caaggugccc     1620

gcgcaaaugg cuguugauau gcagacacuc accccugugg gccgucugau uacugccaac     1680

ccaaucauaa cagagaguac ggaaaauucc cggaugaugu uagagcugga ucccccguuc     1740

ggagacagcu acauugucau cggcgugggg gacaagaaaa ucacccacca uuggcaccga     1800

ucuggaucaa cuauugguaa ggcauuugag gccacaguaa gaggcgcuaa aaggauggcc     1860

gugcuagggg auaccgcaug ggacuucgga aguguuggcg gugucuucaa cagccucggg     1920

aagggcgugc aucagguguu uggagcugcc uucaaauccc uguuuggcgg gaugucuugg     1980

uucucgcaaa uccuuauugg aacauugcug gucuggcucg gccugaauac uaagaacggu     2040

agcauauccc uugcgugucu ggcccucggg ggagcuauga ucuuuuuguc aaccgcaguu     2100

agugccgaug ugggcugcuc uguggacuuc                                      2130


<210>  1806
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short CDS opt

<400>  1806
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauauugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc a                                               2061


<210>  1807
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short CDS opt

<400>  1807
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacacuugu      180

gaugccacca ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacacaac caguacuugg guggucuaug gcacauguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acccuaccgu cccauucuac gaggaaacuc      360

caaacucggu cgcagaccug gcuggaguca cgcgaguaca caaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacuuccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaaccuggg uugauguggu cuuagaacau gguggcugug ugacagugau ggcucaggac      660

aagccaaccg ucgauauuga gcugguuacg acuacagugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacccaa      780

ggggaggccu accuugacaa gcagagcgac acucaguaug ugugcaaaag gacauugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gaccugcgcg      900

aaauuugccu gcucuaagaa aaugacgggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacaccggu     1020

caugaaacag augagaaccg cgcaaagguc gagauuacuc caaauucccc cagagcugaa     1080

gccacccuug gcggguucgg aucacugggc uuggacugug agccuaggac aggucucgau     1140

uuuagugacc uguauuacuu aacuaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca ccggcacacc ccauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac cguugugguc     1320

cugggaagcc aggagggcgc ggugcauacu gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacggccg cuuucacauu uaccaagauc     1500

ccugccgaga cucuccaugg aaccgugaca guugaagugc aguacgcagg caccgacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cucugacacc uguggggagg     1620

uuaauuacgg ccaacccagu uauaaccgag aguacugaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucaca     1740

caccacuggc aucggucugg gucaaccaua ggaaaggcau uugaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacu gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggaccc uccugaugug gcuuggacug     1980

aacgccaaga auggcagcau cuccuugaug ugucucgcuc uggguggggu uuuaauuuuc     2040

cugucaacgg cagucagugc c                                               2061


<210>  1808
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short CDS opt

<400>  1808
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uauuaaggug      420

gagaacugga ucuuuagaaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uuaggugcau cggcguaucc aaccgggacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcac gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcgc      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag aacuuuggug      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcaucc agcccgaaaa ucucgaauau      960

cggauuaugc ugucggugca cgggucucaa cauuccggaa ugaucgguua cgagaccgac     1020

gaagaucgug cgaaggucga ggugacaccu aacucaccac gcgccgaggc aacucuuggc     1080

ggguucggaa gucugggcuu ggacugugaa cccagaaccg gucucgauuu uagcgaccug     1140

uauuacuuaa caaugaauaa caaacacugg cugguacaua aggagugguu ccacgauauc     1200

ccucucccau ggcacgcugg ggccgacacu ggcaccccgc auuggaauaa caaagaagcu     1260

cuuguugagu uuaaggaugc ccacgcaaaa aggcagacag uggucgugcu gggaucccag     1320

gaaggcgccg uccauaccgc uuuggcgggg gcacuagagg ccgagaugga cggagcuaag     1380

ggucggcugu ucucuggcca ccucaagugc cgacugaaaa uggauaaacu ucgccugaag     1440

ggggugagcu auucauugug uacugccgca uuuacguuca caaagguucc cgccgaaacc     1500

cuccauggaa cugugaccgu agagguccag uacgcuggca cagacggucc uugcaaaauu     1560

ccagugcaaa uggccguuga caugcagacc cugacucccg uggggagauu aauaacagca     1620

aauccuguca ucacggaaag uaccgagaac uccaagauga ugcuggaacu agauccaccc     1680

uuuggcgaca gcuacauugu gaucggagua ggcgauaaga aaauaacuca ccacuggcau     1740

aggucugggu caacaauugg aaaggcguuc gaggcuaccg ugcggggugc caaacgcaug     1800

gcaguucucg gcgacacagc cugggauuuc gggagugucg gaggcguguu uaauagccug     1860

gguaagggga uccaccagau cuucggcgcu gccuuuaaau cccuuuucgg aggcaugucu     1920

ugguuuucgc aaauuuugau cgggacucug cucguguggc ugggacuuaa caccaagaac     1980

ggcagcauuu cccugacgug uuuggcacuc ggugggguca ugauauuccu gucaaccgcu     2040

guuagugcc                                                             2049


<210>  1809
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short CDS opt

<400>  1809
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1810
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short CDS opt

<400>  1810
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uauuaaggug      420

gagaacugga ucuuuagaaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uuaggugcau cggcguaucc aaccgggacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcac gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcgc      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag aacuuuggug      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcaucc agcccgaaaa ucucgaauau      960

cggauuaugc ugucggugca cgggucucaa cauuccggaa ugaucgucaa cgacaccggu     1020

uacgagacag augaaaaucg ugcgaaggug gagguaacuc cuaacucacc acgcgccgag     1080

gcaacccuug gcggguucgg aagucugggc uuggacugug aacccagaac aggucucgau     1140

uuuagcgacc uguauuacuu aacuaugaau aacaaacacu ggcugguuca uaaggagugg     1200

uuccacgaua ucccucuccc auggcacgcu ggggccgaca ccggcacacc gcauuggaau     1260

aacaaagaag cucuugugga guuuaaggau gcccacgcaa aaaggcagac cgucgugguc     1320

cugggauccc aggaaggcgc cgugcauacu gcuuuggcgg gggcacuaga ggccgagaug     1380

gacggagcua aggguaagcu guucucuggc caccucaaau gccggcugaa gauggauaaa     1440

cuucgacuga aggggguuag cuauucauug uguacggccg cauuuacauu caccaaagug     1500

cccgccgaaa cucuccaugg aaccguaaca gucgaggugc aguacgcugg caccgacggu     1560

ccuugcaaga uuccaguuca aauggccgug gacaugcaga cucugacacc cgucgggcgc     1620

uuaauaacgg caaauccugu gaucaccgaa aguacugaga acuccaagau gaugcuggaa     1680

cuagauccac ccuuuggcga cagcuacauu guaaucggag ugggcgauaa aaagauaaca     1740

caccacuggc auagaucugg gucaaccauu ggaaaagcgu ucgaggcuac aguuaggggu     1800

gccaagcgga uggcaguccu cggcgacacu gccugggauu ucgggagugu gggaggcgug     1860

uuuaacagcc uggguaaagg gauccaccag aucuucggcg cugccuuuaa gucccuuuuc     1920

ggaggcaugu cuugguuuuc gcaaauuuug aucgggaccc ugcucgucug gcugggacuu     1980

aauacgaaga acggcagcau uucccugacc uguuuggcac ucgguggggu uaugauauuc     2040

cugucaacag cugugagugc c                                               2061


<210>  1811
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short CDS opt

<400>  1811
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccgcaagaaa      300

ggugaggccc gacguuccag aagggcuguc acacuaccau cucauucgac ccggaagcuc      360

caaacgcgcu cacagacuug gcuggaaagc agagaguaua ccaaacaccu uauuaaggug      420

gagaacugga ucuuuaggaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uucggugcau cggcguaucc aaccgcgacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcau gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcga      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagcagaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag gacuuuggug      840

gaucggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcaucc agcccgaaaa ucucgaauau      960

cguauuaugc ugucggugca cgggucucaa cacuccggaa ugaucgucaa cgacaccggu     1020

uacgagacag augaaaaucg cgcgaaggug gagguaacuc cuaacucacc aagagccgag     1080

gcaacccuug gcggguucgg aagucugggc uuggacugug aacccaggac aggucucgau     1140

uuuagcgacc uguauuacuu aacuaugaau aacaaacauu ggcugguuca caaggagugg     1200

uuccaugaua ucccucuccc auggcacgcu ggggccgaca ccggcacacc gcacuggaau     1260

aacaaagaag cucuugugga guuuaaggau gcccaugcaa aacggcagac cgucgugguc     1320

cugggauccc aggaaggcgc cgugcacacu gcuuuggcgg gggcacuaga ggccgagaug     1380

gacggagcua aggguaagcu guucucuggc caucucaaau gccgacugaa gauggauaaa     1440

cuucgccuga aggggguuag cuauucauug uguacggccg cauuuacauu caccaaagug     1500

cccgccgaaa cucuccacgg aaccguaaca gucgaggugc aguacgcugg caccgacggu     1560

ccuugcaaga uuccaguuca aauggccgug gacaugcaga cucugacacc cgucgggaga     1620

uuaauaacgg caaauccugu gaucaccgaa aguacugaga acuccaagau gaugcuggaa     1680

cuagauccac ccuuuggcga cagcuacauu guaaucggag ugggcgauaa aaagauaaca     1740

caucacuggc acaggucugg gucaaccauu ggaaaagcgu ucgaggcuac aguucggggu     1800

gccaagcgca uggcaguccu cggcgacacu gccugggauu ucgggagugu gggaggcgug     1860

uuuaacagcc uggguaaagg gauccaucag aucuucggcg cugccuuuaa gucccuuuuc     1920

ggaggcaugu cuugguuuuc gcaaauuuug aucgggaccc ugcucgucug gcugggacuu     1980

aauacgaaga acggcagcau uucccugacc uguuuggcac ucgguggggu uaugauauuc     2040

cugucaacag cugugagugc c                                               2061


<210>  1812
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short CDS opt

<400>  1812
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uauuaaggug      420

gagaacugga ucuuuagaaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uuaggugcau cggcguaucc aaccgggacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcac gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcgc      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag aacuuuggug      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcaucc agcccgaaaa ucucgaauau      960

cggauuaugc ugucggugca cgggucucaa cauuccggaa ugaccgucaa cgacaucggu     1020

uacgagacag augaaaaucg ugcgaaggug gagguaacuc cuaacucacc acgcgccgag     1080

gcaacccuug gcggguucgg aagucugggc uuggacugug aacccagaac aggucucgau     1140

uuuagcgacc uguauuacuu aacuaugaau aacaaacacu ggcugguuca uaaggagugg     1200

uuccacgaua ucccucuccc auggcacgcu ggggccgaca ccggcacacc gcauuggaau     1260

aacaaagaag cucuugugga guuuaaggau gcccacgcaa aaaggcagac cgucgugguc     1320

cugggauccc aggaaggcgc cgugcauacu gcuuuggcgg gggcacuaga ggccgagaug     1380

gacggagcua aggguaagcu guucucuggc caccucaaau gccggcugaa gauggauaaa     1440

cuucgacuga aggggguuag cuauucauug uguacggccg cauuuacauu caccaaagug     1500

cccgccgaaa cucuccaugg aaccguaaca gucgaggugc aguacgcugg caccgacggu     1560

ccuugcaaga uuccaguuca aauggccgug gacaugcaga cucugacacc cgucgggcgc     1620

uuaauaacgg caaauccugu gaucaccgaa aguacugaga acuccaagau gaugcuggaa     1680

cuagauccac ccuuuggcga cagcuacauu guaaucggag ugggcgauaa aaagauaaca     1740

caccacuggc auagaucugg gucaaccauu ggaaaagcgu ucgaggcuac aguuaggggu     1800

gccaagcgga uggcaguccu cggcgacacu gccugggauu ucgggagugu gggaggcgug     1860

uuuaacagcc uggguaaagg gauccaccag aucuucggcg cugccuuuaa gucccuuuuc     1920

ggaggcaugu cuugguuuuc gcaaauuuug aucgggaccc ugcucgucug gcugggacuu     1980

aauacgaaga acggcagcau uucccugacc uguuuggcac ucgguggggu uaugauauuc     2040

cugucaacag cugugagugc c                                               2061


<210>  1813
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short CDS opt

<400>  1813
accagcaucg gcauuguggg gcugcuccuu acaacugcca uggcugcaga gauaacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uaucucauuc      120

gccaccacau ugggcgucaa caaaugccac guucagauua uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguggaacc ugaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcugug acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uaucaagguu      420

gagaacugga uuuuuagaaa uccgggguuc acacuggugg cagucgccau cgcuugguug      480

cucggaaguu ccacuucuca gaaggugaua uaccugguaa ugauccugcu cauugccccc      540

gcguauagca ucaggugcau uggcguuucc aaccgggacu uuguggaagg caugucaggg      600

ggaaccuggg ucgauguggu guuagagcac gguggcugug ucacaguuau ggcacaggac      660

aaaccuaccg uggauaucga acugguaacg acuacaguga guaauauggc cgagguccgc      720

agcuacugcu acgaggcuuc uauauccgac auggccucag auagccgaug uccaacccaa      780

ggggaagcau aucuugacaa gcagagugac acucaguacg ugugcaaaag aacauugguu      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gaccugcgcu      900

aaauuuacgu gcuccaagaa gaugaccggc aaaagcauuc agcccgagaa ucucgaauau      960

cggaucaugc ugucggucca cgggucucaa cauuccggaa ugauugugaa cgacgagaau     1020

cgugccaagg uagaaguuac accuaacuca ccacgcgcgg aggccacucu ugguggcuuc     1080

gggagucugg gauuggauug ugagcccaga accggccucg acuuuagcga ucuguacuau     1140

uuaacaauga auaacaaaca cuggcuggug cauaaggaau gguuccacga caucccucuc     1200

ccauggcacg caggggcuga uacugggacc ccgcauugga auaacaaaga ggcccuuguc     1260

gaauuuaagg acgcucacgc caaaaggcag acaguggucg ugcugggcuc ccaggaggga     1320

gcaguucaua ccgccuuggc uggcgcgcua gaagcagaga uggauggggc caagggacgg     1380

cuguucucug gucaccucaa gugccgacug aaaauggaca aacuucgccu gaagggcgug     1440

agcuacucau uguguacugc ugccuuuacg uucacaaagg uacccgcaga gacccuccau     1500

gggacuguca ccguggaagu ucaguaugcc ggaacagaug gcccuugcaa agugccagcu     1560

caaauggccg ucgacaugca gacccugacu cccgugggua gauuaaucac agcaaauccu     1620

guaauuacgg agaguaccga aaacuccaag augaugcugg agcuagaccc acccuuuggg     1680

gauagcuaca uagugaucgg cguuggagac aagaaaauua cucaccacug gcauaggucu     1740

ggcucaacaa ucgggaaggc guucgaagcu accguccggg gagccaaacg cauggcagug     1800

cucggugaua cagccuggga cuucggcagu guggguggag ucuuuaacag ccugggcaag     1860

gguauacacc agauuuucgg ggcugccuuu aaaucccuuu ucggcggaau gucuugguuu     1920

ucgcaaaucu ugaucggcac ucugcucguu uggcuggggc uuaauaccaa gaacggaagc     1980

auuucccuga cguguuuggc acucggcggu gugaugaucu uccugucaac cgcugugagu     2040

gcc                                                                   2043


<210>  1814
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short CDS opt

<400>  1814
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uauuaaggug      420

gagaacugga ucuuuagaaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uuaggugcau cggcguaucc aaccgggacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcac gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcgc      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag aacuuuggug      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcaucc agcccgagaa ucucgaauau      960

cggauuaugc ugucggugca cgggucucaa cauuccggaa ugaucgguua cgagaccgac     1020

gaaaaccgug cgaaggucga ggugacaccu aauucaccac gcgccgaggc aacucuuggc     1080

ggguucggaa gucugggcuu ggauugugaa cccagaaccg gucucgacuu uagcgaucug     1140

uauuacuuaa caaugaacaa uaaacacugg cugguacaua aggagugguu ccacgacauc     1200

ccucucccau ggcacgcugg ggccgauacu ggcaccccgc auuggaacaa uaaagaagcu     1260

cuuguugagu uuaaggacgc ccacgcaaaa aggcagacag uggucgugcu gggaucccag     1320

gaaggcgccg uccauaccgc uuuggcgggg gcacuagagg ccgagaugga uggagcuaag     1380

gguaagcugu ucucuggcca ccucaaaugc cggcugaaga uggacaaacu ucgacugaag     1440

ggggugagcu auucauugug uacugccgca uuuacguuca caaaaguucc cgccgaaacc     1500

cuccauggaa cugugaccgu agagguccag uacgcuggca cagauggucc uugcaagauu     1560

ccagugcaaa uggccguuga caugcagacc cugacucccg uggggcgcuu aauaacagca     1620

aacccuguca ucacggaaag uaccgaaaau uccaagauga ugcuggaacu agacccaccc     1680

uuuggcgaua gcuacauugu gaucggagua ggcgacaaaa agauaacuca ccacuggcau     1740

agaucugggu caacaauugg aaaagcguuc gaggcuaccg ugaggggugc caagcggaug     1800

gcaguucucg gcgauacagc cugggacuuc gggagugucg gaggcguguu uaacagccug     1860

gguaaaggga uccaccagau cuucggcgcu gccuuuaagu cccuuuucgg aggcaugucu     1920

ugguuuucgc aaauuuugau cgggacucug cucguguggc ugggacuuaa caccaagaau     1980

ggcagcauuu cccugacgug uuuggcacuc ggugggguca ugauauuccu gucaaccgcu     2040

guuagugcc                                                             2049


<210>  1815
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short CDS opt

<400>  1815
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguagggg gauucaccag aucuucggcg ccgcguuuaa guccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaaa auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1816
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short CDS opt

<400>  1816
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccgggcug gcuuuggcag ccgcugccau ugcguggcuc      480

cugggaagca guacauccca gaaagugauc uaucugguca ugauacucuu aaucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu ucguagaggg cauguccggg      600

ggaacuuggg uugauguggu ccuggaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcuuguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu acuuggacaa gcagagcgac acccaguaug ugugcaaaag gacucugguc      840

gaucggggau ggggcaaugg uugugggcua uuuggaaagg gcagucucgu gacaugcgcg      900

aaauucgccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccuggaguac      960

cguauuaugc uuagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucugg gcggguuugg aucauugggc cucgacugug agccuaggac cggucuggau     1140

uucagugacu uauauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuucacgaua ucccacuucc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacugguaga guucaaggau gcccacgcua aacggcagac aguugugguc     1320

uugggaagcc aggaaggcgc ggugcauacc gcacuagccg gggcucugga ggccgaaaug     1380

gacggagcaa agggucgacu cuccucuggc caccugaaau gccgccuuaa gauggauaag     1440

cugagauuga aaggggucag cuauucacuc uguacugccg cuuuuacguu cacaaagauc     1500

ccugccgaga cccugcaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga ccuuaacucc uguggggagg     1620

cugauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuagag     1680

cucgaucccc cguuuggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau ucgaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu gggcgauaca gcaugggacu uugggagugu gggaggcgcu     1860

cuuaauagcu uggguaaggg gauucaccag aucuucggcg ccgcguucaa aucccuguuu     1920

ggaggcaugu cuugguucuc gcaaauccuc auugggacuc ugcuuaugug gcugggauug     1980

aacaccaaga auggcagcau cucccucaug ugucuggccu uagguggggu ucugauuuuu     2040

cuaucaacgg cugucagugc a                                               2061


<210>  1817
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short CDS opt

<400>  1817
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggu aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaucag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1818
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short CDS opt

<400>  1818
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacacccaga cucugacacc uguggggagg     1620

uuaauuacgg ccaacccagu uauaaccgag aguacugaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucaca     1740

caccacuggc aucggucugg gucaaccaua ggaaaggcau uugaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacu gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggaccc uccugaugug gcuuggacug     1980

aacacgaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaaccg cugucagugc a                                               2061


<210>  1819
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short CDS opt

<400>  1819
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagauccga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc a                                               2061


<210>  1820
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short CDS opt

<400>  1820
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacuggcgca aauggcuguc gacaugcaga ccuuaacucc cguggggagg     1620

cugauuacag ccaacccugu uauaacggag aguaccgaaa acuccaagau gaugcuagag     1680

cucgauccac ccuucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu gggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cuuaauagcu uggguaaggg gauucaccag aucuucggcg ccgcguuuaa aucccuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccuc auugggacuc ugcuuaugug gcugggauug     1980

aacaccaaga auggcagcau cucccucaug ugucuggccu uagguggggu ucugauuuuc     2040

cuaucaacgg cugucagugc a                                               2061


<210>  1821
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short CDS opt

<400>  1821
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuacc cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacacaac caguacuugg guggucuaug gcacauguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acccuaccgu cccauucuac gaggaaacuc      360

caaacucggu cgcagaccug gcuggaguca cgcgaguaca caaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacuuccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaaccuggg uugauguggu cuuagaacau gguggcugug ugacagugau ggcucaggac      660

aagccaaccg ucgauauuga gcugguuacg acuacagugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacccaa      780

ggggaggccu accuugacaa gcagagcgac acucaguaug ugugcaaaag gacauugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gaccugcgcg      900

aaauuugccu gcucuaagaa aaugacgggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacaccggu     1020

caugaaacag augagaaccg cgcaaagguc gagauuacuc caaauucccc cagagcugaa     1080

gccacccuug gcggguucgg aucacugggc uuggacugug agccuaggac aggucucgau     1140

uuuagugacc uguauuacuu aacuaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca ccggcacacc ccauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac cguugugguc     1320

cugggaagcc aggagggcgc ggugcauacu gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacggccg cuuucacauu uaccaagauc     1500

ccugccgaga cucuccaugg aaccgugaca guugaagugc aguacgcagg caccgacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cucugacacc uguggggagg     1620

uuaauuacgg ccaacccagu uauaaccgag aguacugaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucaca     1740

caccacuggc aucggucugg gucaaccaua ggaaaggcau uugaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacu gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggaccc uccugaugug gcuuggacug     1980

aacacgaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaaccg cugucagugc a                                               2061


<210>  1822
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short CDS opt

<400>  1822
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg ucgugagugc u                                               2061


<210>  1823
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short CDS opt

<400>  1823
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aagugcaugg guggucuaug gcaccuguca ucacaaaaag      300

ggugaagccc gccgaagccg uagagcugug acucuaccgu cccauucuac aaggaaacuc      360

caaacccggc cccagacgug gcuggagucg cgcgaguaca cuaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa cccuggguuc gcacuggccg cugccgcgau ugcaugguug      480

cucggaucaa gcaccaguca gaaagugauc uaucugguca ugauacugcu caucgcccca      540

gcuuacucca uucggugcau cggcgugucu aaucgcgacu uuguagaggg caugagcggg      600

ggaacauggg uugauguggu cuuagaacau gguggcugug ugacugugau ggcccaggac      660

aagcccaccg ucgauauuga gcugguuaca accacggugu ccaacauggc agagguacga      720

ucauacugcu augaagcuag uaucagcgac auggccucug auuccagaug uccuacucaa      780

ggggaggcgu accuugacaa gcagucagac acacaguaug ugugcaaaag gaccuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagccuagu gacuugcgcc      900

aaauuugcau gcaguaagaa aaugacaggc aagucuauac agccagaaaa ccucgaguac      960

cguauuaugc uguccguuca cgggagccaa cacucgggaa ugaucgugaa ugacaccggu     1020

caugaaacgg augagaaccg cgcuaagguc gagauuaccc ccaauucucc uagagccgaa     1080

gcuacacuug gcggguucgg aucccugggc uuggacugug agccaaggac uggucucgau     1140

uuuucagacc uguauuacuu aaccaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccgcuccc cuggcacgcc ggggcagaca caggcacucc ucauuggaac     1260

aauaaagagg cccuuguaga auuuaaggau gcucacgcga aacggcagac cguugugguc     1320

cugggaaguc aggagggcgc agugcauaca gccuuggcug gggcccuaga ggcagaaaug     1380

gacggagcca agggucgacu gagcuccggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga aaggggucuc uuauagcuug uguaccgcug ccuucacuuu uacgaagauc     1500

ccagcagaga cacuccaugg aaccgugacu guugaagugc aguacgcggg caccgacggu     1560

cccugcaaag uaccugcuca aauggccguc gacaugcaga cacugacccc aguggggagg     1620

uuaauuacug caaaccccgu uauaacagag ucaacggaaa acaguaagau gaugcuggag     1680

cuagauccgc cuuucggcga cuccuacauc gugauuggag ucggcgagaa aaagaucacc     1740

caccacuggc aucggagcgg gucuacuaua ggaaaggccu uugaagcuac agugcgcggu     1800

gccaaaagaa uggcaguacu cggcgauacc gcuugggacu ucgggucagu gggaggcgcc     1860

cugaauaguc uugguaaggg gauucaccag aucuucggcg cggccuuuaa gagcuuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug auugggacac uccugaugug gcuuggacug     1980

aacacuaaga auggcucgau cagcuugaug ugucucgcuc uggguggggu uuuaauuuuc     2040

cuguccaccg cagucucagc c                                               2061


<210>  1824
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short CDS opt

<400>  1824
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccaa aaaggugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacag      780

ggggaggccu accuugacaa acaaagcgac acccaguaug ugugcaagag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaaag gcagucuagu gacaugcgcg      900

aaguuugccu gcucuaaaaa gaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcag cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaaguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaagcacu ggcuggugca uaaagaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaggagg cacuuguaga auuuaaagau gcccacgcua agcggcaaac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa aaggucgacu guccucuggc caccucaagu gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaaaauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaagg uacccgcgca gauggcuguc gacaugcaaa cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaaaau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa gaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaagcau uugaagccac cgugcgcggu     1800

gcuaagagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaagg gauucaccag aucuucggcg ccgcguuuaa guccuuguuc     1920

ggaggcaugu cuugguuuuc gcagauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaaa auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1825
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short CDS opt

<400>  1825
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacacggca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucca uucgcugcau cggcgugucu aaucgagacu uuguagaggg caugagcggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu ccaacauggc cgagguaaga      720

ucauacugcu augaagcaag uaucagcgac auggcuucug auuccaggug ucccacacaa      780

ggggaggccu accuugacaa gcagucagac acccaguaug ugugcaaacg gacuuugguc      840

gaucguggau ggggcaaugg uugugggcug uucggaaagg gcagccuagu gacaugcgcg      900

aaauuugccu gcaguaagaa aaugaccggc aagucuauac agccugaaaa ccucgaguac      960

cgcauuaugc uguccguuca cgggagccaa cacucgggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaacag agcaaagguc gagauuacac caaauucucc cagggcugaa     1080

gccacucuug gcggguucgg aucccugggc uuggacugug agccucggac cggucucgau     1140

uuuucagacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacgacagac aguugugguc     1320

cugggaaguc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgccu gagcuccggc caccucaaau gcagacugaa gauggauaaa     1440

cuuaggcuga aaggggucuc uuauagcuug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggcgg     1620

uuaauuacag ccaacccagu uauaacggag ucaaccgaaa acaguaagau gaugcuggag     1680

cuagaucccc cguucggcga cuccuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucgcagcgg gucuacaaua ggaaaggcau uugaagccac cgugagaggu     1800

gcuaaaagga uggccguacu cggcgauaca gcaugggacu ucgggucagu gggaggcgcu     1860

cugaauaguc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa gagcuuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcucgau cagcuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cuguccacgg cugucucagc a                                               2061


<210>  1826
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short CDS opt

<400>  1826
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaaccc aggagggcgc ggugcauacu gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gagcuccggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga aaggggucuc uuauagcuug uguacggccg cuuucacauu uaccaagauc     1500

ccugccgaga cucuccaugg aaccgugaca guugaagugc aguacgcagg caccgacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cucugacacc uguggggagg     1620

uuaauuacgg ccaacccagu uauaaccgag ucaacugaaa acaguaagau gaugcuggag     1680

cuagaucccc cguucggcga cuccuacauc gugauuggag ucggcgagaa aaagaucaca     1740

caccacuggc aucggagcgg gucuaccaua ggaaaggcau uugaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacu gcaugggacu ucgggucagu gggaggcgcu     1860

cugaauaguc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa gagcuuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug auugggaccc uccugaugug gcuuggacug     1980

aacacgaaga auggcucgau cagcuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cuguccaccg cugucucagc a                                               2061


<210>  1827
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short CDS opt

<400>  1827
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucuu uacggugcau uggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu ccuggaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauaucga gcuuguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag cauuucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu acuuggacaa gcagagcgac acccaguaug ugugcaaaag gacucugguc      840

gaucggggau ggggcaaugg uugugggcua uucggaaagg gcagucucgu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccaucc agccugaaaa ccuggaguac      960

cguauaaugc uuagcguuca cgggucgcaa cacucuggaa ugauugugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagaucacac caaauucccc cagagcugaa     1080

gccacucugg gcggguucgg aucauugggc cucgacugug agccuaggac cggucuggau     1140

uuuagugacu uauauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccacuucc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacugguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

uugggaagcc aggagggcgc ggugcauacc gcacuagccg gggcucugga ggccgaaaug     1380

gacggagcaa agggucgacu cuccucuggc caccugaaau gccgccuuaa gauggauaag     1440

cugagauuga aaggggucag cuauucacuc uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccugcaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga ccuuaacucc uguggggagg     1620

cugaucacag ccaacccagu uauuacggag aguaccgaaa acuccaagau gaugcuagag     1680

cucgaucccc cguucggcga cagcuacaua gugaucggag ucggcgagaa aaagauuacu     1740

caccacuggc aucggucugg gucaacaauc ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu gggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cuuaauagcu uggguaaggg gauacaccag auuuucggcg ccgcguuuaa aucccuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccuc aucgggacuc ugcuuaugug gcugggauug     1980

aacaccaaga auggcagcau uucccucaug ugucuggccu uagguggggu ucugaucuuc     2040

cuaucaacgg cugucagugc a                                               2061


<210>  1828
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short CDS opt

<400>  1828
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaauc      420

gaaaauugga uuuuuaggaa ccccggguuc gcucuggcag ccgcugccau cgcgugguug      480

cucggaagca guacauccca gaaaguuaua uaucugguga ugauccugcu cauugcaccc      540

gccuacucua uccggugcau uggcgucagc aaucgcgacu uuguggaggg cauguccggg      600

ggaacuuggg uagauguugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaagcc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag ccaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu guuaauauuc     2040

cugucaacgg cuguuagugc a                                               2061


<210>  1829
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short CDS opt

<400>  1829
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga uccugaggaa ccccggguuu gcuuuggcag ccgcugccau ugcguggcuc      480

cugggaagca guacauccca gaaagugauc uaucugguca ugauacucuu aaucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu ucguagaggg cauguccggg      600

ggaacuuggg uugauguggu ccuggaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcuuguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu acuuggacaa gcagagcgac acccaguaug ugugcaaaag gacucugguc      840

gaucggggau ggggcaaugg uugugggcua uuuggaaagg gcagucucgu gacaugcgcg      900

aaauucgccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccuggaguac      960

cguauuaugc uuagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucugg gcggguuugg aucauugggc cucgacugug agccuaggac cggucuggau     1140

uucagugacu uauauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuucacgaua ucccacuucc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacugguaga guucaaggau gcccacgcua aacggcagac aguugugguc     1320

uugggaagcc aggaaggcgc ggugcauacc gcacuagccg gggcucugga ggccgaaaug     1380

gacggagcaa agggucgacu cuccucuggc caccugaaau gccgccuuaa gauggauaag     1440

cugagauuga aaggggucag cuauucacuc uguacugccg cuuuuacguu cacaaagauc     1500

ccugccgaga cccugcaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga ccuuaacucc uguggggagg     1620

cugauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuagag     1680

cucgaucccc cguuuggcga cagcuacauc gugauuggaa ucggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau ucgaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu gggcgauaca gcaugggacu uugggagugu aggaggcgcu     1860

cuuaauagcu uggguaaggg gauccaccag aucuucggcg ccgcguucaa aucccuguuu     1920

ggaggcaugu cuugguucuc gcaaauucuc aucgggacuc ugcuuaugug gcugggauug     1980

aacaccaaga auggcagcau uucccucaug ugucuggccu uagguggggu gcugauauuu     2040

cuaucaacgg cuguuagugc a                                               2061


<210>  1830
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short CDS opt

<400>  1830
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuuca accacgguga guaacauggc cgagguacga      720

agcuacugcu augaagcaac uaucucugac auagcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcguuca cgggucgcaa cacucuggaa ugauugugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac agcgguugug     1320

cugggaagcc aggagggcgc aguccauacc gccuuggcug gggcccuaga ggcagaaaug     1380

gacggagcca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugcug ccuucacguu uacaaagauu     1500

ccugcagaga cccuccaugg aacugucacc guggaaguuc aguacgcggg cacagacggu     1560

ccaugcaaag ugcccgcuca aauggccgua gacaugcaga cccugacucc ugucgggagg     1620

uuaauaacag caaacccagu gaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu guuaucggag ugggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggccu uugaagcuac cguccgcggu     1800

gccaaaagaa uggcagugcu cggcgauaca gcuugggacu ucgggagugu aggaggcgcc     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg cggccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgcuc uggguggggu guuaauauuc     2040

cugucaacgg caguuagugc c                                               2061


<210>  1831
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short CDS opt

<400>  1831
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaacugc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga ccuuaacucc ugucgggagg     1620

cugauuacag ccaacccagu gauaacggag aguaccgaaa acuccaagau gaugcuagag     1680

cucgaucccc cguucggcga cagcuacauc guuauuggag ugggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu gggcgauaca gcaugggacu ucgggagugu aggaggcgcu     1860

cuuaauagcu uggguaaggg gauucaccag aucuucggcg ccgcguuuaa aucccuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccuc auugggacuc ugcuuaugug gcugggauug     1980

aacaccaaga auggcagcau cucccucaug ugucuggccu uagguggggu gcugauuuuc     2040

cuaucaacgg cuguuagugc a                                               2061


<210>  1832
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short CDS opt

<400>  1832
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaucgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu gugaucggag ucggcgagaa aaagauaacc     1740

caccacuggc aucggucugg gucaacuauu ggaaaggcau uugaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacc gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacac uccugaugug gcuuggacug     1980

aacacuaaga auggcagcau uuccuugaug ugucucgccc uggguggggu uuuaauauuc     2040

cugucaaccg cugucagugc a                                               2061


<210>  1833
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short CDS opt

<400>  1833
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggaguca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaag     1440

uuaagacuga aaggggugag cuauucauug uguacugcag ccuucacguu uacaaagauc     1500

ccugcugaga cccuccaugg aacuguuacc guggaaguac aguacgccgg cacagacggu     1560

ccaugcaaag uccccgcaca aauggcggug gacaugcaga cccugacucc uguugggagg     1620

uuaauuacag cuaacccagu gauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gucauuggag ugggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggccu uugaagcaac cguacgcggu     1800

gccaaaagaa uggcugugcu cggcgauaca gccugggacu ucgggagugu uggaggcgca     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg cugccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuucaug uguuuggcgc ucgguggggu ccugauuuuu     2040

cuuucaacgg ccgugagugc u                                               2061


<210>  1834
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short CDS opt

<400>  1834
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa aggguaaacu guccucuggc caccucaagu gccgacugaa gauggauaaa     1440

cuucgccuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaaaauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaagg uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggaga     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaaaau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa gaagaucacu     1740

caccacuggc auaggucugg gucaacaaua ggaaaagcau uugaagccac cgugcggggu     1800

gcuaagcgca uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaagg gauucaccag aucuucggcg ccgcguuuaa guccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaaa auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1835
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short CDS opt

<400>  1835
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaaggaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa caaaugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaauaccac aaguaccugg guggucuaug gcacuuguca ucacaagaaa      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaagcuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaaacaccu uauuagaguu      420

gaaaacugga ucuuuaggaa ucccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaggugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaccgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaauauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa acagagcgac acccaguaug ugugcaagag gacuuugguc      840

gaucggggau ggggcaacgg uugugggcug uucggaaaag gcagucuagu gacaugcgcg      900

aaguuugccu gcucuaaaaa gaugaccggc aaguccauac agccugaaaa ucucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa cgacacgggu     1020

caugaaaccg augagaaucg cgcaaaaguc gagauuacac caaacucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaau aacaagcacu ggcuggugca uaaagaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaau     1260

aacaaggagg cacuuguaga auuuaaagau gcccacgcua agcggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa aaggucgacu guccucuggc caccucaagu gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaaaauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaagg uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaauccagu uauaacggag aguaccgaaa acuccaaaau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa gaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaagcau uugaagccac cgugcgcggu     1800

gcuaagagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaacagcc uugguaaagg gauucaccag aucuucggcg ccgcguuuaa guccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aauaccaaaa acggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1836
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short CDS opt

<400>  1836
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggag gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga agccgagaug     1380

gauggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggacaaa     1440

cuuagacuga aaggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaaa cccuccaugg aacugugacc guugaggugc aguacgcagg cacagauggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggaa aguaccgaga acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgaaaa gaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaggccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgacaca gcaugggauu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1837
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short CDS opt

<400>  1837
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccgcucuug gcggguucgg aucacugggc uuggacugug agccuaggac uggucucgau     1140

uuuagugacc uguauuacuu aaccaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcagaca caggcacucc ccauuggaac     1260

aauaaagagg cccuuguaga auuuaaggau gcucacgcga aacggcagac cguugugguc     1320

cugggaagcc aggagggcgc agugcauaca gccuuggcug gggcccuaga ggcagaaaug     1380

gacggagcca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguaccgcug ccuucacuuu uacgaagauc     1500

ccugcagaga cacuccaugg aaccgugacu guugaagugc aguacgcggg caccgacggu     1560

ccaugcaaag uacccgcuca aauggccguc gacaugcaga cacugacccc uguggggagg     1620

uuaauuacug caaacccagu uauaacagag aguacggaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacc     1740

caccacuggc aucggucugg gucaacuaua ggaaaggccu uugaagcuac agugcgcggu     1800

gccaaaagaa uggcaguacu cggcgauacc gcuugggacu ucgggagugu gggaggcgcc     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg cggccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaacacug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgcuc uggguggggu uuuaaucuuc     2040

cugucaacgg cagucagugc c                                               2061


<210>  1838
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short CDS opt

<400>  1838
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cgcggacggu     1560

ccaugcaaag uacccgcuca aauggccguc gacaugcaga cacugacccc uguggggagg     1620

uuaauuacug caaacccagu uauaacagag aguacggaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacc     1740

caccacuggc aucggucugg gucaacuaua ggaaaggccu uugaagcuac agugcgcggu     1800

gccaaaagaa uggcaguacu cggcgauacc gcuugggacu ucgggagugu gggaggcgcc     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg cggccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacac uccugaugug gcuuggacug     1980

aacacuaaga auggcagcau cuccuugaug ugucucgcuc uggguggggu uuuaauuuuc     2040

cugucaaccg cagucagugc c                                               2061


<210>  1839
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short CDS opt

<400>  1839
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcauua      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu ccuggaacau gguggcugug ugaccgugau ggcucaggac      660

aagccuacag ucgauauuga gcuuguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug uccaacacaa      780

ggggaggccu acuuggacaa gcagagcgac acccaguaug ugugcaaaag gacucugguc      840

gaucggggau ggggcaaugg uugugggcua uucggaaagg gcagucucgu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agcccgaaaa ccuggaguac      960

cguauuaugc uuagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac cuaauucccc aagagcugaa     1080

gccacucugg gcggguucgg aucauugggc cucgacugug agcccaggac cggucuggau     1140

uuuagugacu uauauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua ucccucuucc auggcacgcu ggggccgaca cuggcacccc gcauuggaac     1260

aauaaagagg cacugguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

uugggaagcc aggagggcgc ggugcauacc gcacuagccg gggcucugga ggccgaaaug     1380

gacggagcaa agggucgacu cuccucuggc caccugaaau gccgccuuaa gauggauaag     1440

cugagauuga aaggggucag cuauucacuc uguacugccg cuuucacguu uacaaagauc     1500

cccgccgaga cccugcaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccuugcaaag uaccagcgca aauggcuguc gacaugcaga ccuuaacucc cguggggagg     1620

cugauuacag ccaacccugu uauaacggag aguaccgaaa acuccaagau gaugcuagag     1680

cucgauccac ccuucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu gggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cuuaauagcu uggguaaggg gauucaccag aucuucggcg ccgcguuuaa aucccuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccuc auugggacuc ugcuuaugug gcugggauug     1980

aacaccaaga auggcagcau cucccucaug ugucuggccu uagguggggu ucugauuuuc     2040

cuaucaacgg cugucagugc a                                               2061


<210>  1840
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short CDS opt

<400>  1840
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac augguguccg auucaagaug ucccacacaa      780

ggggaggcuu accuugacaa gcagagcgac acccaguaug ucugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu uacaugcgcc      900

aaauuugcgu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcgugca cgggucgcaa cacucuggaa ugaucgucaa ugacacgggu     1020

caugaaaccg augagaaccg cgccaaggug gagauuacac caaauucccc cagagcagaa     1080

gcuacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcugguaca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcugaca cuggcacccc ucauuggaac     1260

aauaaagagg cccuuguuga auuuaaggau gcacacgcca aacggcagac aguggucgug     1320

cugggaagcc aggagggcgc uguccauacc gcguuggcag gggcccuaga ggcugaaaug     1380

gacggagcca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggugag cuauucauug uguacugcag ccuucacguu uacaaagauc     1500

ccugcugaga cccuccaugg aacuguuacc guggaaguac aguacgccgg cacagacggu     1560

ccaugcaaag uccccgcaca aauggcggug gacaugcaga cccugacucc uguugggagg     1620

uuaauuacag cuaacccagu gauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gucauuggag ugggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggccu uugaagcaac cguacgcggu     1800

gccaaaagaa uggcugugcu cggcgauaca gccugggacu ucgggagugu uggaggcgca     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg cugccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuugaug ugucucgcgc uggguggggu cuuaauuuuc     2040

cugucaacgg ccgugagugc u                                               2061


<210>  1841
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short CDS opt

<400>  1841
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcauuagc aaucgcgacu uuguggaggg cauguccggg      600

ggaacuuggg uagauguugu guuagaacau gguggcugug ucaccgugau ggcucaggac      660

aagccaacag uggauaucga gcuggucacc acgacuguuu caaacauggc cgaggugcga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcgugca cgggucgcaa cacucuggaa ugauuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaaggug gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aauccugggc uuggacugug agccuaggac cggucucgau     1140

uuuucagacc uguauuacuu aacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua uuccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuugugga auuuaaggau gcccacgcua aacggcagac aguaguugug     1320

cugggaaguc aggagggcgc gguccauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gagcuccggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga aagggguguc uuauagcuug uguacugccg cuuucacguu uacaaagaua     1500

ccugccgaga cccuccaugg aacugucacc guggaaguuc aguacgcagg cacagacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cccugacucc ugucgggagg     1620

uuaaucacag ccaacccagu gauuacggag ucaaccgaaa acaguaagau gaugcuggag     1680

cuagaucccc cguucggcga cuccuacauc guuauaggag ugggcgagaa aaagauuacu     1740

caccacuggc aucggagcgg gucuacaauc ggaaaggcau uugaagccac cguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauaca gcaugggacu ucgggucagu aggaggcgcu     1860

cugaauaguc uugguaaggg gauccaccag auuuucggcg ccgcguuuaa gagcuuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcucgau cagcuugaug ugucucgccc uggguggggu guuaauauuc     2040

cuguccacgg cuguuucagc a                                               2061


<210>  1842
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short CDS opt

<400>  1842
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacacuugu      180

gaugccacca ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacacaac caguacuugg guggucuaug gcacauguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acccuaccgu cccauucuau uaggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uaucagaguu      420

gaaaauugga uuuuuaggaa ccccggguuc gcucuggcag ccgcugccau cgcgugguug      480

cucggaagca guacauccca gaaagugaua uaucugguca ugauccugcu cauugcaccc      540

gccuacucua uccggugcau uggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauaucga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag cauaucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauuc agccugaaaa ccucgaguac      960

cguaucaugc ugagcguuca cgggucgcaa cacucuggaa ugauugugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauu     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauaacag ccaacccagu uaucacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauu gugaucggag ucggcgagaa aaagauaacu     1740

caccacuggc aucggucugg gucaacaauu ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauccaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauucug aucgggacuc uccugaugug gcuuggacug     1980

aacgccaaga auggcagcau uuccuugaug ugucucgcuc uggguggggu uuuaauauuc     2040

cugucaaccg cagucagugc c                                               2061


<210>  1843
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short CDS opt

<400>  1843
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac aacgcagccg acgugccgug acacuaccgu cccauucuac cagaaaacuc      360

cagacgaggu cgcagacuug gcuggaguca cgggaguaca ccaagcaccu uauucgcguu      420

gaaaauugga ucuuuagaaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uuaggugcau cggcgugagc aaucgggacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaagac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacgc      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucacgaug ucccacacag      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag aacuuugguc      840

gauaggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac aaccugaaaa ccucgaguac      960

cggauuaugc ugagcguuca cgggucgcag cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg ugcaaagguc gagauuacac caaauucccc ucgcgcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuagaac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacacc ccauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aaaggcagac cguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucggcu guccucuggc caccucaaau gccgacugaa gauggauaaa     1440

cuucgccuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aauacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca gauggcuguc gacaugcaga cccugacucc uguggggaga     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc auaggucugg gucaacaaua ggaaaggcau uugaagccac cgugcggggu     1800

gcuaaacgca uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccaa aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcagauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1844
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short CDS opt

<400>  1844
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caagcucggu cgcagacgug gcuggaguca cgcgaguaca cuaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcacuggccg cugccgcgau ugcaugguug      480

cucggaagca guaccuccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcuuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacauggg uugauguggu cuuagaacau gguggcugug ugacugugau ggcccaggac      660

aagccaaccg ucgauauuga gcugguuaca accacggugu caaacauggc agagguacga      720

aguuacugcu augaagcuag caucucugac auggccuccg auucaagaug ucccacucaa      780

ggggaggcgu accuugacaa gcagagcgac acacaguaug ugugcaaaag gaccuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacuugcgcc      900

aaauuugcau gcucuaagaa aaugacaggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacaccggu     1020

caugaaacgg augagaaccg cgcuaagguc gagauuaccc caaauucccc cagagccgaa     1080

gcuacacuug gcggguucgg aucacugggc uuggacugug agccuaggac uggucucgau     1140

uuuagugacc uguauuacuu aaccaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcagaca caggcacucc ccauuggaac     1260

aauaaagagg cccuuguaga auuuaaggau gcucacgcga aacggcagac cguugugguc     1320

cugggaagcc aggagggcgc agugcauaca gccuuggcug gggcccuaga ggcagaaaug     1380

gacggagcca agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguaccgcug ccuucacuuu uacgaagauc     1500

ccugcagaga cacuccaugg aaccgugacu guugaagugc aguacgcggg caccgacggu     1560

ccaugcaaag uacccgcuca aauggccguc gacaugcaga cacugacccc uguggggagg     1620

uuaauuacug caaacccagu uauaacagag aguacggaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacc     1740

caccacuggc aucggucugg gucaacuaua ggaaaggccu uugaagcuac agugcgcggu     1800

gccaaaagaa uggcaguacu cggcgauacc gcuugggacu ucgggagugu gggaggcgcc     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg cggccuuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacac uccugaugug gcuuggacug     1980

aacacuaaga auggcagcau cuccuugaug ugucucgcuc uggguggggu uuuaauuuuc     2040

cugucaaccg cagucagugc c                                               2061


<210>  1845
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short CDS opt

<400>  1845
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cgggugaagc uauuucauuc      120

cccaccacau ugggcaugaa caagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugaguuacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaucccg uagagccgug acacuaccgu cucauucgac caggaaacuc      360

caaacgcggu cacagacuug gcuggagagc cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaacugga ucuuuaggaa ucccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaaguu ccacaucuca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacagca uucggugcau cggcgugucc aaccgcgacu uuguagaggg caugucaggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacuguga guaauauggc cgagguacga      720

agcuacugcu augaagcauc uaucuccgac auggcuucag auagcagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagugac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcg      900

aaauuugccu gcuccaagaa aaugaccggc aagagcauac agccugaaaa ucucgaguac      960

cguauuaugc ugucgguuca cgggucucaa cacuccggaa ugaucgugaa cgacacgggu     1020

caugaaaccg augagaaucg cgcaaagguc gagauuacac caaacucacc cagagcugaa     1080

gccacucuug gcggguucgg aagucugggc uuggacugug agccuaggac cggucucgau     1140

uuuagcgacc uguauuacuu aacaaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaau     1260

aacaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggauccc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga aaggggucuc auauaguuug uguacugccg cuuucacguu uuccaagauc     1500

ccugccgaga cacuccaugg aaccgugacu gaagagguuc aguacgcagg caccgacggu     1560

ccaugcaaag ugcccgcgca aauggcugua gacaugcaga cacugacccc ugucgggagg     1620

uuaauuacug ccaauccagu gauaacagaa agcacggaga acucuaagau gaugcuggag     1680

cuagaucccc cguucggcga cucauacauc guuauuggag ugggcgaaaa gaagaucacc     1740

caccacuggc aucggagugg gagcacuaua ggaaaggcau uugaggccac aguccgcggu     1800

gcuaaaagaa uggccgugcu cggcgauacc gcaugggacu ucggguccgu aggaggcgcu     1860

cugaacucuc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa aucguuguuc     1920

ggaggcauga gcugguuuuc ccaaauccug auugggacac uccugaugug gcuuggacug     1980

aauacuaaga acggcucaau caguuugaug ugucucgccc uggguggggu guuaauuuuc     2040

cugucuaccg cuguuagcgc a                                               2061


<210>  1846
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short CDS opt

<400>  1846
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cgggugaagc uauuucauuc      120

cccaccacau ugggcaugaa caagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugaguuacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaucccg uagagccgug acacuaccgu cucauucgac caggaaacuc      360

caaacgcggu cacagacuug gcuggagagc cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaacugga ucuuuaggaa ucccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaaguu ccacaucuca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacagca uucggugcau cggcgugucc aaccgcgacu uuguagaggg caugucaggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacuguga guaauauggc cgagguacga      720

agcuacugcu augaagcauc uaucuccgac auggcuucag auagcagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagugac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcg      900

aaauuugccu gcuccaagaa aaugaccggc aagagcauac agccugaaaa ucucgaguac      960

cguauuaugc ugucgguuca cgggucucaa cacuccggaa ugaucgugaa cgacacgggu     1020

caugaaaccg augagaaucg cgcaaagguc gagauuacac caaacucacc cagagcugaa     1080

gccacucuug gcggguucgg aagucugggc uuggacugug agccuaggac cggucucgau     1140

uuuagcgacc uguauuacuu aacaaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacacu ggggcugaca ccggcacacc ccauuggaau     1260

aacaaagagg cccuuguaga auuuaaggau gcacacgcca aacggcagac cguugugguc     1320

cugggauccc aggagggcgc ugugcauacu gcguuggcag gggcccuaga ggcugaaaug     1380

gacggagcca agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaag     1440

cugagacuga aaggggucuc auauaguuug uguacggcag ccuucacauu uaccaagauc     1500

ccugcugaga cucuccaugg aaccgugaca guugaagugc aguacgccgg caccgacggu     1560

ccaugcaaag uacccgcaca aauggcgguc gacaugcaga cucugacacc uguggggagg     1620

uuaauuacgg cuaauccagu uauaaccgag ggcacugaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggaga cagcuacauc gugauuggcg ucggggagaa aaagaucaca     1740

caccacuggc aucggucugg aucaaccaua gguaaggccu uugaagcaac agugcgcggc     1800

gccaaaagaa uggcuguacu cggggauacu gccugggacu ucggaagugu gggcgguguu     1860

cugaacagcc uugggaaggg cauucaccag aucuucggag cagcuuuuaa auccuuguuc     1920

ggcgggaugu cuugguuuuc gcaaauccug auuggaaccc uccugaugug gcuuggccug     1980

aauacgaaga acgguagcau cuccuugaug ugucucgccc uugggggagu cuuaauuuuc     2040

cugucaaccg cggugagugc c                                               2061


<210>  1847
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short CDS opt

<400>  1847
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cgggugaagc uauuucauuc      120

cccaccacau ugggcaugaa caagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugaguuacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaucccg uagagccgug acacuaccgu cucauucgac caggaaacuc      360

caaacgcggu cacagacuug gcuggagagc cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaacugga ucuuuaggaa ucccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaaguu ccacaucuca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacagca uucggugcau cggcgugucc aaccgcgacu uuguagaggg caugucaggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacuguga guaauauggc cgagguacga      720

agcuacugcu augaagcauc uaucuccgac auggcuucag auagcagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagugac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcg      900

aaauuugccu gcuccaagaa aaugaccggc aagagcauac agccugaaaa ucucgaguac      960

cguauuaugc ugucgguuca cgggucucaa cacuccggaa ugaucgugaa cgacacgggu     1020

caugaaaccg augagaaucg cgcaaagguc gagauuacac caaacucacc cagagcugaa     1080

gccacucuug gcggguucgg aagucugggc uuggacugug agccuaggac cggucucgau     1140

uuuagcgacc uguauuacuu aacaaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaau     1260

aacaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggauccc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucuc auauaguuug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaauccagu uauaacggag uccaccgaaa acagcaagau gaugcuggag     1680

cuagaucccc cguucggcga cucuuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucagg gaguacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagcgu gggaggcgcu     1860

cugaacuccc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa aucuuuguuc     1920

ggaggcaugu cgugguuuag ccaaauccug auugggacuc uccugaugug gcuuggacug     1980

aauaccaaga acggcuccau cucauugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugaguacgg cugucucugc a                                               2061


<210>  1848
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short CDS opt

<400>  1848
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcuguuga ggugacgaga       60

aggggaaacg cauacuauau guaccuggac cgguccgaug ccggugaagc gauuucuuuc      120

cccaccacaa ugggcaugaa uaagugcuau auacagauca uggacuuggg gcacaugugu      180

gaugcuacua ugucauacga gugcccuaug uuagacgaag gaguagagcc agaugacgug      240

gauuguuggu gcaacaccac aaguaccugg gucguguaug gcacuuguca ucacaaaaag      300

ggugaagccc gccgaagccg uagagcaguu acacugccgu cccauucuac caggaaacua      360

caaacgcggu cgcagacuug gcucgaguca cgcgaguaca ccaagcaccu gauuagagug      420

gaaaauugga ucuuuaggaa ccccggguuc gcccuugcug cagccgcuau ugccuggcug      480

uugggaagca guacauccca gaaagucauc uaucucguga ugauacugcu gaucgcgccu      540

gcauacucua uucggugcau cggcguaagc aaucgcgacu uuguugaggg cauguccggg      600

ggaacuuggg uggaugucgu gcucgaacau gguggcugug ugaccgucau ggcccaggac      660

aagccaacag uugauauuga guuagugacc acgacuguau caaacauggc ugaggugcga      720

aguuacugcu augaagccag caucucugac auggcauccg auucaagaug ucccacacaa      780

ggggaggcuu accuggacaa gcagagcgac acccaguaug ucugcaaaag gacucuugug      840

gaucggggau ggggcaaugg uuguggguug uucggaaagg gcagucuggu uacaugcgcc      900

aaauuugcgu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccuagaguac      960

cguauuaugc ucagcgugca cgggucgcaa cacucuggaa ugaucgucaa ugacacgggu     1020

caugaaaccg augagaaccg cgccaaggug gagauuacac caaauucccc cagagcagaa     1080

gcuacucugg gcggguucgg aucacuuggc cuggacugug agccuaggac cgguuuggau     1140

uuuagugacc ucuauuaccu gacaaugaac aauaaacacu gguuaguaca uaaggaaugg     1200

uuccacgaua ucccacugcc guggcacgcc ggggcugaca cuggcacacc ccauuggaac     1260

aauaaagagg cccucguuga auuuaaggau gcacacgcca aacggcagac cguggucgug     1320

cuuggaagcc aggagggcgc uguccauacc gcgcuggcag gggccuugga ggcugaaaug     1380

gacggagcca agggucgacu auccucuggc caccugaaau gccgccucaa gauggauaag     1440

cugagacuua aaggggugag cuauucacug uguacugcag ccuucacguu uacaaagauc     1500

ccugcugaga ccuugcaugg aacuguuacc guggaaguac aguacgccgg cacagacggu     1560

ccaugcaaag uccccgcaca aauggcggug gacaugcaga cccucacucc uguugggagg     1620

cugauuacag cuaacccagu gauaacggag aguaccgaaa acuccaagau gauguuagag     1680

cuggaucccc cguucggcga cagcuacauc gucauuggag ugggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggccu uugaagcaac cguacgcggu     1800

gccaaaagaa uggcugugcu aggcgauaca gccugggacu ucgggagugu uggaggcgca     1860

cucaauagcc uggguaaggg gauucaccag aucuucggcg cugccuuuaa aucccuuuuc     1920

ggaggcaugu cuugguuuuc gcaaaucuug auugggacuc ugcucgucug gcugggacuu     1980

aacaccaaga auggcagcau cucccugaug uguuuggcgc ucgguggggu gcugauuuuc     2040

uuaucaacgg ccgugagugc u                                               2061


<210>  1849
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short CDS opt

<400>  1849
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cgggugaagc uauuucauuc      120

cccaccacau ugggcaugaa caagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugaguuacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaucccg uagagccgug acacuaccgu cucauucgac caggaaacuc      360

caaacgcggu cacagacuug gcuggagagc cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaacugga ucuuuaggaa ucccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaaguu ccacaucuca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacagca uucggugcau cggcgugucc aaccgcgacu uuguagaggg caugucaggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacuguga guaauauggc cgagguacga      720

agcuacugcu augaagcauc uaucuccgac auggcuucag auagcagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagugac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcg      900

aaauuugccu gcuccaagaa aaugaccggc aagagcauac agccugaaaa ucucgaguac      960

cguauuaugc ugucgguuca cgggucucaa cacuccggaa ugaucgugaa cgacacgggu     1020

caugaaaccg augagaaucg cgcaaagguc gagauuacac caaacucacc cagagcugaa     1080

gccacucuug gcggguucgg aagucugggc uuggacugug agccuaggac cggucucgau     1140

uuuagcgacc uguauuacuu aacaaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaau     1260

aacaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggauccc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucuc auauaguuug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaauccagu uauaacggag uccaccgaaa acagcaagau gaugcuggag     1680

cuagaucccc cguucggcga cucuuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucagg gaguacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagcgu gggaggcgcu     1860

cugaacuccc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa aucuuuguuc     1920

ggaggcaugu cgugguuuag ccaaauccug auugggacuc uccugaugug gcuuggacug     1980

aauaccaaga acggcuccau cucauugguu ugucucgccc uggguggggu cuuaauuuuc     2040

cugaguacgg cugugucugc a                                               2061


<210>  1850
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short CDS opt

<400>  1850
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cgggugaagc uauuucauuc      120

cccaccacau ugggcaugaa caagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugaguuacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaucccg uagagccgug acacuaccgu cucauucgac caggaaacuc      360

caaacgcggu cacagacuug gcuggagagc cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaacugga ucuuuaggaa ucccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaaguu ccacaucuca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacagca uucggugcau cggcgugaac aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau cuccuugguu ugucucgccc uggguggggu cuuaauuuuc     2040

cugucaacgg cugugagugc a                                               2061


<210>  1851
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short CDS opt

<400>  1851
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcuguuga ggugacgaga       60

aggggauccg cauacuauau guaccuggac cggucugaug ccggugaagc gauuucauuc      120

cccaccacau ugggcaugaa caagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugcuacua ugaguuacga gugcccuaug cuggacgaag gaguagagcc agaugacgug      240

gauuguuggu gcaauaccac aagcaccugg gucguguaug gcacuuguca ucacaaaaag      300

ggugaagccc gccgaucccg uagagcaguu acacuaccgu cucauucgac caggaaacuc      360

caaacgcggu cacagacuug gcuggagagc cgcgaguaca ccaagcaccu uauuagagug      420

gaaaacugga ucuuuaggaa ucccggguuc gcccuggcug cagccgcuau ugccugguug      480

cucggaaguu ccacaucuca gaaagucauc uaucugguga ugauacugcu caucgcgccu      540

gcauacagca uucggugcau cggcguaucc aaccgcgacu uuguugaggg caugucaggg      600

ggaacuuggg uggaugucgu guuagaacau gguggcugug ugaccgucau ggcccaggac      660

aagccagcug uugauauuga gcuggugaca accacgguaa guaauauggc cgaggugcga      720

agcuacugcu augaagcauc uaucuccgac auggcuucag auagcagaug ucccacucaa      780

ggggaggccu accuugacaa gcagagugac acacaguaug ucugcaaaag gaccuuggug      840

gaucggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacuugcgcg      900

aaauuugccu gcuccaagaa aaugacaggc aagagcauac agccugaaaa ucucgaguac      960

cguauuaugc ugucggugca cgggucucaa cacuccggaa ugaucgucaa cgacaccggu     1020

caugaaacgg augagaaucg cgcaaaggug gagauuaccc caaacucacc cagagcugaa     1080

gccacacuug gcggguucgg aagucugggc uuggacugug agccuaggac uggucucgau     1140

uuuagcgacc uguauuacuu aaccaugaau aacaaacacu ggcugguaca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca caggcacucc ccauuggaau     1260

aacaaagagg cacuuguuga auuuaaggau gcccacgcua aacggcagac cguggucgug     1320

cugggauccc aggagggcgc gguccauaca gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga aggggguguc auauaguuug uguaccgccg cuuucacuuu uacgaagauc     1500

ccugccgaga cacuccaugg aaccguuacu guggaaguac aguacgcagg caccgacggu     1560

ccaugcaaag uccccgcgca aauggcugug gacaugcaga cacugacccc uguugggagg     1620

uuaauuacug ccaauccagu gauaacagag uccacggaaa acagcaagau gaugcuggag     1680

cuagaucccc cguucggcga cucuuacauc gucauuggag ugggcgagaa aaagaucacc     1740

caccacuggc aucggucagg gaguacuaua ggaaaggcau uugaagccac aguacgcggu     1800

gcuaaaagaa uggccgugcu cggcgauacc gcaugggacu ucgggagcgu uggaggcgcu     1860

cugaacuccc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa aucuuuguuc     1920

ggaggcaugu cgugguuuag ccaaauccug auugggacac uccuggucug gcuuggacug     1980

aauacuaaga acggcuccau cucauugacc ugucucgccc uggguggggu guuaauuuuc     2040

cugaguacgg cugugucugc a                                               2061


<210>  1852
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short CDS opt

<400>  1852
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcuguuga ggugacgaga       60

aggggauccg cauacuauau guaccuggac cggucugaug ccggugaagc gauuucauuc      120

cccaccacau ugggcaugaa caagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugcuacua ugaguuacga gugcccuaug cuggacgaag gaguagagcc agaugacgug      240

gauuguuggu gcaauaccac aagcaccugg gucguguaug gcacuuguca ucacaaaaag      300

ggugaagccc gccgaucccg uagagcaguu acacuaccgu cucauucgac caggaaacuc      360

caaacgcggu cacagacuug gcuggagagc cgcgaguaca ccaagcaccu uauuagagug      420

gaaaacugga ucuuuaggaa ucccggguuc gcccuggcug cagccgucau ugcuugguug      480

cucggaaguu ccacaucuca gaaagugauc uaucugguaa ugauacugcu caucgccccu      540

gcguacagca uucggugcau cggcguuucc aaccgcgacu uuguggaggg caugucaggg      600

ggaacuuggg ucgauguggu guuagaacau gguggcugug ucaccguuau ggcacaggac      660

aagccaacag uggauauuga gcugguaacc acgacuguga guaauauggc cgagguccga      720

agcuacugcu augaggcuuc uaucuccgac auggccucag auagcagaug ucccacacaa      780

ggggaagcau accuugacaa gcagagugac acccaguaug ugugcaaaag gacuuugguu      840

gaucggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcu      900

aaauuugccu gcuccaagaa aaugaccggc aagagcauac agccugaaaa ucucgaguac      960

cguauuaugc ugucggucca cgggucucaa cacuccggaa ugaucgugaa cgacacgggu     1020

caugaaaccg augagaaucg cgcgaaggua gagauuacac caaacucacc cagagccgaa     1080

gcaacucuug gcggguucgg aagucugggc uuggacugug agccuaggac cggucucgau     1140

uuuagcgacc uguauuacuu aacaaugaau aacaaacacu ggcugguuca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacacc ccauuggaau     1260

aacaaagagg cucuugugga auuuaaggau gcccacgcaa aacggcagac cgucgugguc     1320

cugggauccc aggagggcgc cgugcauacc gcuuuggcgg gggcacuaga ggccgaaaug     1380

gacggagcua agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga aggggguuuc auauaguuug uguacugccg cauucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guagaagucc aguacgcugg cacagacggu     1560

ccaugcaaag ugcccgccca aauggcaguu gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag cgaauccagu cauaacggag uccaccgaaa acagcaagau gaugcuggag     1680

cuagaucccc cguucggcga cucuuacauc gugauuggag uaggcgagaa aaagaucacu     1740

caccacuggc aucggucagg gaguacaaua ggaaaggcuu uugaagccac cgugcgcggu     1800

gcaaaaagaa uggccguucu cggcgauaca gcuugggacu ucgggagcgu cggaggcgcc     1860

cugaacuccc uugguaaggg gauucaccag aucuucggcg cagcuuuuaa aucuuuguuc     1920

ggaggcaugu cgugguuuag ccaaauccug auugggacuc uccuggugug gcuuggacug     1980

aauaccaaga acggcuccau cucauugacg ugucucgccc uggguggggu guuaauuuuc     2040

cugaguaccg cggucucugc c                                               2061


<210>  1853
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short CDS opt

<400>  1853
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcuguuga ggugacgaga       60

aggggauccg cauacuauau guaccuggac cggucugaug ccggugaagc gauuucauuc      120

cccaccacau ugggcguaaa caagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugcuacua ugaguuacga gugcccuaug cuggacgaag gaguggagcc agaugacguc      240

gauuguuggu gcaauaccac aagcaccugg gugguuuaug gcacuuguca ucacaaaaag      300

ggugaagccc gccgaucccg uagagcagug acacuaccgu cucauucgac caggaaacuc      360

caaacgcggu cacagacuug gcuggagagc cgcgaguaca ccaagcaccu uauuagaguc      420

gaaaacugga ucuuuaggaa ucccggguuc gcccuggcug cagccgcuau ugccugguug      480

cucggaaguu ccacaucuca gaaagugauc uaucugguaa ugauacugcu caucgcgccu      540

gcauacagca uucggugcau cggcguuucc aaccgcgacu uuguggaggg caugucaggg      600

ggaacuuggg ucgauguggu guuagaacau gguggcugug ucaccguuau ggcccaggac      660

aagccaacag uggauauuga gcugguaacc acgacuguga guaauauggc ugagguccga      720

agcuacugcu augaagccuc uaucuccgac auggcaucag auagcagaug ucccacacaa      780

ggggaggcuu accuugacaa gcagagugac acccaguaug ugugcaaaag gacuuugguu      840

gaucggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcc      900

aaauuugcgu gcuccaagaa aaugaccggc aagagcauac agccugaaaa ucucgaguac      960

cguauuaugc ugucggucca cgggucucaa cacuccggaa ugaucgugaa cgacacgggu     1020

caugaaaccg augagaaucg cgccaaggua gagauuacac caaacucacc cagagcagaa     1080

gcuacucuug gcggguucgg aagucugggc uuggacugug agccuaggac cggucucgau     1140

uuuagcgacc uguauuacuu aacaaugaau aacaaacacu ggcugguuca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcugaca cuggcacacc ccauuggaau     1260

aacaaagagg cccuugugga auuuaaggau gcacacgcca aacggcagac cgucgugguc     1320

cugggauccc aggagggcgc ugugcauacc gcguuggcag gggcccuaga ggcugaaaug     1380

gacggagcca agggucgacu gucuagcggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga aggggguuuc auauaguuug uguacugcag ccuucacguu uacaaagauc     1500

ccugcugaga cccuccaugg aacugugacc guagaagucc aguacgccgg cacagacggu     1560

ccaugcaaag ugcccgcaca aauggcgguu gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag cuaauccagu cauaacggag uccaccgaaa acagcaagau gaugcuggag     1680

cuagaucccc cguucggcga cucuuacauc gugauuggag uaggcgauaa aaagaucacu     1740

caccacuggu aucggucagg gaguacaaua ggaaaggccu uugaggcaac cgugcgcggu     1800

gccaaaagaa uggcuguucu cggcgacaca gccugggauu ucgggagcgu cggaggcgca     1860

cugaacuccc uugguaaggg gauucaucag aucuucggcg cugccuuuaa aucuuuguuc     1920

ggaggcaugu cgugguuuag ccaaauccug auugggacuc uccuggugug gcuuggacug     1980

aauaccaaga acggcuccau cucauugacg ugucucgcgc uggguggggu guuaauuuuc     2040

cugaguaccg ccgucucugc u                                               2061


<210>  1854
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short CDS opt

<400>  1854
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcuguuga ggugacgaga       60

aggggauccg cauacuauau guaccuggac cggucugaug ccgguaaggc gauuucauuc      120

cccaccacau ugggcguaaa caaaugcuau auacagauca uggacuuagg gcacaugugu      180

gaugcuacua ugaguuacga augcccuaug cuggacgagg gaguggaacc agaugacguc      240

gauuguuggu gcaauaccac aagcaccugg gugguuuaug gcacuuguca ucacaagaaa      300

ggugaggccc gccgaucccg uagagcagug acacuaccgu cucauucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaca ccaaacaccu uauuagaguc      420

gagaacugga ucuuuaggaa ucccggguuc gcccuggcug cagccgcuau ugccugguug      480

cucggaaguu ccacaucuca gaaggugauc uaucugguaa ugauacugcu caucgcgccu      540

gcauacagca uucggugcau cggcguuucc aaccgcgacu uuguggaagg caugucaggg      600

ggaacuuggg ucgauguggu guuagagcau gguggcugug ucaccguuau ggcccaggac      660

aagccaacag uggauauuga acugguaacc acgacuguga guaauauggc ugagguccga      720

agcuacugcu augaggccuc uaucuccgac auggcaucag auagcagaug ucccacacaa      780

ggggaggcuu accuugacaa acagagugac acccaguaug ugugcaagag gacuuugguu      840

gaucggggau ggggcaacgg uugugggcug uucggaaaag gcucucuagu gacaugcgcc      900

aaguuugcgu gcuccaaaaa gaugaccggc aagagcauac agccugaaaa ucucgaauac      960

cguauuaugc ugucggucca cgggucucaa cacuccggaa ugaucgugaa cgacauuggu     1020

caugagacgg augaaaaucg cgccaaagua gagaucaccc caaacucacc cagagcagag     1080

gcuacacuug gcggguucgg aagucugggc uuggacugug aaccuaggac uggucucgau     1140

uuuagcgacc uguauuacuu aaccaugaau aacaagcacu ggcugguuca uaaagagugg     1200

uuccacgaua ucccacuccc guggcacgcc ggggcugaca caggcacucc ccauuggaau     1260

aacaaggaag cccuugugga guuuaaagau gcacacgcca agcggcagac cgucgugguc     1320

cugggauccc aggaaggcgc ugugcauaca gcguuggcag gggcccuaga ggcugagaug     1380

gacggagcca aaggucgacu gucuagcggc caccucaagu gccgccugaa gauggauaaa     1440

cuuagacuga aggggguuuc auauaguuug uguaccgcag ccuucacuuu uacgaaaauu     1500

ccugcugaaa cacuccaugg aaccgugacu guagaggucc aguacgccgg caccgacggu     1560

ccaugcaagg ugcccgcaca aauggcgguu gacaugcaga cacugacccc uguggggagg     1620

uuaauaacug cuaauccagu caucacagaa uccacggaga acagcaaaau gaugcuggaa     1680

cuagaucccc cguucggcga cucuuacauu gugaucggag uaggcgauaa gaagauaacc     1740

caccacuggc aucggucagg gaguacuauu ggaaaagccu uugaggcaac agugcgcggu     1800

gccaagagaa uggcuguucu cggcgacacc gccugggauu ucgggagcgu cggaggcgca     1860

cugaacuccc uugguaaagg gauccaccag aucuucggcg cugccuuuaa gucuuuguuc     1920

ggaggcaugu cgugguuuag ccaaauucug aucgggacac uccuggugug gcuuggacug     1980

aauacuaaaa acggcuccau uucauugacc ugucucgcgc uggguggggu guuaauauuc     2040

cugaguacgg ccgucucugc u                                               2061


<210>  1855
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short CDS opt

<400>  1855
accagcaucg gcauuguggg gcugcuccuu acaacugcca uggcugcaga gauaacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uaucucauuc      120

gccaccacau ugggcgucaa caaaugccac guucagauua uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguggaacc ugaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcugug acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uaucaagguu      420

gagaacugga uuuuuagaaa uccgggguuc acacuggugg cagucgccau cgcuugguug      480

cucggaaguu ccacuucuca gaaggugaua uaccugguaa ugauccugcu cauugccccc      540

gcguauagca ucaggugcau uggcguuucc aaccgggacu uuguggaagg caugucaggg      600

ggaaccuggg ucgauguggu guuagagcac gguggcugug ucacaguuau ggcacaggac      660

aaaccuaccg uggauaucga acugguaacg acuacaguga guaauauggc cgagguccgc      720

agcuacugcu acgaggcuuc uauauccgac auggccucag auagccgaug uccaacccaa      780

ggggaagcau aucuugacaa gcagagugac acucaguacg ugugcaaaag aacauugguu      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gaccugcgcu      900

aaauuuacgu gcuccaagaa gaugaccggc aaaagcauuc agcccgagaa ucucgaauau      960

cggaucaugc ugucggucca cgggucucaa cauuccggaa ugauugugaa cgacacaggu     1020

cacgagacug augaaaaucg ugccaaggua gagguuaccc cuaacucacc acgcgcggag     1080

gccacacuug gcggguucgg aagucugggc uuggacugug aacccagaac uggucucgau     1140

uuuagcgacc uguacuauuu aaccaugaau aacaaacauu ggcuggugca caaggagugg     1200

uuccacgaua ucccucuccc auggcaugca ggggcugaca caggcacccc gcacuggaau     1260

aacaaagaag cccuugucga guuuaaggau gcucaugcca aaaggcagac uguggucgug     1320

cugggauccc aggaaggcgc aguucacacg gccuuggcug gggcgcuaga ggcagagaug     1380

gacggagcca agggucggcu guucucuggc caucucaagu gccgacugaa aauggauaaa     1440

cuucgccuga agggggugag cuacucauug uguacagcug ccuuuaccuu cacuaaggua     1500

cccgcagaaa cccuccacgg aacagucacc guggagguuc aguaugccgg cacugacggu     1560

ccuugcaaag ugccagcuca aauggccguc gacaugcaga cacugacgcc cguggggaga     1620

uuaaucaccg caaauccugu aauuacugaa aguacagaga acuccaagau gaugcuggaa     1680

cuagauccac ccuuuggcga cagcuacaua gugaucggag uuggcgauaa gaaaauuacc     1740

caccauuggc acaggucugg gucaacaauc ggaaaggcgu ucgaggcuac uguccggggu     1800

gccaaacgca uggcagugcu cggcgacacc gccugggauu ucgggagugu gggaggcguc     1860

uuuaacagcc uggguaaggg gauacaucag auuuucggcg cugccuuuaa aucccuuuuc     1920

ggaggcaugu cuugguuuuc gcaaaucuug aucgggacgc ugcucguuug gcugggacuu     1980

aauaccaaga acggcagcau uucccugaca uguuuggcac ucgguggggu gaugaucuuc     2040

cugucaacug cugugagugc c                                               2061


<210>  1856
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short CDS opt

<400>  1856
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccaacu ugggcgugaa uaaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacaa ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaacacuac cagcacaugg guaguguacg gcaccuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acucuaccau cucacucgac aaggaagcuc      360

caaacccggu cacagacgug gcuggaaagc cgcgaguaua cuaaacaucu uauuaaggug      420

gagaauugga ucuuuagaaa cccgggguuc gcacuggccg cuguugccau agcgugguug      480

cucggaaguu ccaccucuca gaaggugauc uaccugguca ugauucugcu caucgcaccc      540

gccuauagca uuaggugcau cggcgugucc aaucgggacu uuguagaagg caugucaggg      600

ggaacauggg uugauguggu cuuagagcac gguggcugug ugacugugau ggcucaggac      660

aaaccuaccg ucgauauaga acugguuaca accacgguga guaacauggc cgagguacgc      720

agcuacugcu acgaggcauc uauuuccgac auggcuucag auagccgaug uccaacucaa      780

ggggaagccu aucuugacaa gcagagugac acacaguacg ugugcaaaag aaccuugguc      840

gauaggggau ggggcaaugg uugugggcug uucggaaagg gcucucuagu gacuugcgcg      900

aaauuuacau gcuccaagaa gaugaccggc aaaagcaucc agcccgagaa ccucgaauau      960

cggauuaugc ugucgguuca cgggucucaa cauuccggaa ugaucgugaa ugacaucggu     1020

cacgagacgg augaaaaccg ugccaagguc gaggugaccc cuaauucacc acgcgcagag     1080

gcuacacuug gcggguucgg aagucugggc uuggacugug aacccagaac uggucucgau     1140

uuuagcgacc uguacuauuu aaccaugaac aauaaacauu ggcugguaca caaggagugg     1200

uuccacgaua uuccucuccc auggcaugcc ggggcugaca caggcacucc gcacuggaac     1260

aauaaagaag cccuuguuga guuuaaggau gcacaugcca aaaggcagac cguggucgug     1320

cugggauccc aggaaggcgc uguccacaca gcguuggcag gggcccuaga ggcugagaug     1380

gacggagcca agggucggcu guucucuggc caucucaagu gccgacugaa aauggauaaa     1440

cuucgccuga agggggugag cuacucauug uguaccgcag ccuuuacuuu cacgaagguu     1500

cccgcugaaa cacuccacgg aaccgugacu guagaggucc aguaugccgg caccgacggu     1560

ccuugcaaag ugccagcaca aauggcgguu gacaugcaga cacugacccc cguggggaga     1620

uuaauaacug cuaacccugu caucacagaa aguacggaga acuccaagau gaugcuggaa     1680

cuagauccac ccuuuggcga cagcuacauu gugaucggag uaggcgauaa gaaaauaacc     1740

caccauuggc acaggucugg gucaacuauu ggaaaggccu ucgaggcaac agugcggggu     1800

gccaaacgca uggcuguucu cggcgacacc gccugggauu ucgggagugu cggaggcgug     1860

uuuaauagcc uggguaaggg ggugcaucag aucuucggcg cagcuuuuaa aucccuuuuc     1920

ggaggcaugu cuugguuuuc gcaaaucuug auugggacac ugcucgucug gcugggacuu     1980

aacacuaaga auggcagcau cucccugacc uguuuggccc ucgguggggu uaugauuuuc     2040

cugucaacgg cggugagugc c                                               2061


<210>  1857
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short CDS opt

<400>  1857
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uauuaaggug      420

gagaacugga ucuuuagaaa uccgggguuc gcacugguug ccguggcuau agccugguug      480

cucggaaguu ccacaucuca gaaggucauc uaccugguga ugauucugcu caucgcgccc      540

gcauauagca uuaggugcau cggcguaucc aaccgggacu uuguugaagg caugucaggg      600

ggaacuuggg uggaugucgu guuagagcac gguggcugug ugaccgucau ggcccaggac      660

aaaccuacag uugauauaga acuggugacc acgacuguaa guaauauggc ugaggugcgc      720

agcuacugcu acgaggccuc uauuuccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ucugcaaaag aacuuuggug      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu uacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcaucc agcccgaaaa ucucgaauau      960

cggauuaugc ugucggugca cgggucucaa cauuccggaa ugaucgucaa cgacaucggu     1020

cacgagaccg augaaaaucg ugcgaaggug gagguaacac cuaacucacc acgcgccgag     1080

gcaacucuug gcggguucgg aagucugggc uuggacugug aacccagaac cggucucgau     1140

uuuagcgacc uguacuauuu aacaaugaau aacaaacauu ggcugguuca caaggagugg     1200

uuccacgaua uuccucuccc auggcaugcu ggggccgaca cuggcacccc gcacuggaau     1260

aacaaagaag cucuugugga guuuaaggau gcccaugcaa aaaggcagac agucgugguc     1320

cugggauccc aggaaggcgc cgugcacacc gcuuuggcgg gggcacuaga ggccgagaug     1380

gacggagcua agggucggcu guucucuggc caucucaagu gccgacugaa aauggauaaa     1440

cuucgccuga aggggguuag cuacucauug uguacugccg cauuuacguu cacaaaggug     1500

cccgccgaaa cccuccacgg aacuguaacc gucgaggugc aguaugcugg cacagacggu     1560

ccuugcaaag uuccagccca aauggcagug gacaugcaga cccugacucc cgucgggaga     1620

uuaauaacag cgaauccugu gaucacggaa aguaccgaga acuccaagau gaugcuggaa     1680

cuagauccac ccuuuggcga cagcuacauu guaaucggag ugggcgauaa gaaaauaacu     1740

caccauuggc acaggucugg gucaacaauu ggaaaggcuu ucgaggccac cguucggggu     1800

gcaaaacgca uggccguccu cggcgacaca gcuugggauu ucgggagugu gggaggcgug     1860

uuuaacagcc uggguaaggg gauccaucag aucuucggcg ccgcauuuaa aucccuuuuc     1920

ggaggcaugu cuugguuuuc gcaaauuuug aucgggacuc ugcucgucug gcugggacuu     1980

aauaccaaga acggcagcau uucccugacg uguuuggcuc ucgguggggu uaugauauuc     2040

cugucaaccg ccgugagugc g                                               2061


<210>  1858
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short CDS opt

<400>  1858
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccaacu ugggcgugaa uaaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacaa ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaacacuac cagcacaugg guaguguacg gcaccuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcuguc acucuaccau cucacucgac aaggaagcuc      360

caaacccggu cacagacgug gcuggaaagc cgcgaguaua cuaaacaucu uauuaaggug      420

gagaauugga ucuuuagaaa cccgggguuc gcacuggccg cuguugccau agcgugguug      480

cucggaaguu ccaccucuca gaaggugauc uaccugguca ugauucugcu caucgcaccc      540

gccuauagca uuaggugcau cggcgugucc aaucgggacu uuguagaagg caugucaggg      600

ggaacauggg uugauguggu cuuagagcac gguggcugug ugacugugau ggcucaggac      660

aaaccuaccg ucgauauaga acugguuaca accacgguga guaacauggc cgagguacgc      720

agcuacugcu acgaggcauc uauuuccgac auggcuucag auagccgaug uccaacucaa      780

ggggaagccu aucuugacaa gcagagugac acacaguacg ugugcaaaag aaccuugguc      840

gauaggggau ggggcaaugg uugugggcug uucggaaagg gcucucuagu gacuugcgcg      900

aaauuuacau gcuccaagaa gaugaccggc aaaagcaucc agcccgagaa ccucgaauau      960

cggauuaugc ugucgguuca cgggucucaa cauuccggaa ugaucgugaa ugacgagaac     1020

cgugccaagg ucgaagugac gccuaauuca ccacgcgcag aggcuacccu ugguggcuuc     1080

gggagucugg gauuggauug ugagcccaga acaggccucg acuuuagcga ucuguacuau     1140

uuaacuauga acaauaaaca cuggcuggua cauaaggaau gguuccacga caucccucuc     1200

ccauggcacg ccggugcuga uaccgggaca ccgcauugga acaauaaaga ggcccuuguu     1260

gaauuuaagg acgcacacgc caaaaggcag acuguggucg ugcugggcuc ccaggaggga     1320

gcuguccaua ccgcguuggc aggcgcccua gaagcugaga uggauggggc caagggacgg     1380

cuguucucug gucaccucaa gugccgacug aaaauggaca aacuucgccu gaagggcgug     1440

agcuacucau uguguacagc agccuuuacc uucacuaagg uucccgcuga gacgcuccau     1500

gggacaguga ccguagaagu ccaguaugcc ggaacugaug gcccuugcaa agugccagca     1560

caaauggcgg uugacaugca gacccugaca cccgugggua gauuaauuac cgcuaacccu     1620

gucauaacug agaguacaga aaacuccaag augaugcugg agcuagaccc acccuuuggg     1680

gauagcuaca ucgugauugg cguaggagac aagaaaauca cgcaccacug gcauaggucu     1740

ggcucaacca uagggaaggc cuucgaagca acugugcggg gagccaaacg cauggcuguu     1800

cucggugaua cagccuggga cuucggcagu gucgggggag uguuuaauag ccugggcaag     1860

gguauucacc agaucuucgg ggcagcuuuu aaaucccuuu ucggcggaau gucuugguuu     1920

ucgcaaaucu ugauuggcac ccugcucgug uggcuggggc uuaacacaaa gaauggaagc     1980

aucucccuga cuuguuuggc ccucggcggu gucaugauuu uccugucaac cgcgguuagu     2040

gcc                                                                   2043


<210>  1859
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short CDS opt

<400>  1859
accagcaucg gcauuguggg gcugcuccuu acaacugcca uggcugcaga gauaacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uaucucauuc      120

gccaccacau ugggcgucaa caaaugccac guucagauua uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguggaacc ugaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcugug acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uaucaagguu      420

gagaacugga uuuuuagaaa uccgggguuc gcacuggugg ccgucgcuau cgccugguug      480

cucggaaguu ccacaucuca gaaggugaua uaccugguaa ugauccugcu cauugcgccc      540

gcauauagca ucaggugcau uggcguuucc aaccgggacu uuguggaagg caugucaggg      600

ggaacuuggg ucgauguggu guuagagcac gguggcugug ucaccguuau ggcccaggac      660

aaaccuacag uggauaucga acugguaacc acgacuguga guaauauggc ugagguccgc      720

agcuacugcu acgaggccuc uauauccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ugugcaaaag aacuuugguu      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcauuc agcccgaaaa ucucgaauau      960

cggaucaugc ugucggucca cgggucucaa cauuccggaa ugauugugaa cgacaccggu     1020

cacgagacag augaaaaucg ugcgaaggua gagguuacuc cuaacucacc acgcgccgag     1080

gcaacccuug gcggguucgg aagucugggc uuggacugug aacccagaac aggucucgau     1140

uuuagcgacc uguacuauuu aacuaugaau aacaaacauu ggcuggugca caaggagugg     1200

uuccacgaua ucccucuccc auggcaugcu ggggccgaca ccggcacacc gcacuggaau     1260

aacaaagaag cucuugucga guuuaaggau gcccaugcaa aaaggcagac cguggucgug     1320

cugggauccc aggaaggcgc cguucacacu gcuuuggcgg gggcacuaga ggccgagaug     1380

gacggagcua agggucggcu guucucuggc caucucaagu gccgacugaa aauggauaaa     1440

cuucgccuga agggggugag cuacucauug uguacggccg cauuuacauu caccaaggua     1500

cccgccgaaa cucuccacgg aaccgucaca guggagguuc aguaugcugg caccgacggu     1560

ccuugcaaag ugccagccca aauggcaguc gacaugcaga cucugacacc cguggggaga     1620

uuaaucacgg cgaauccugu aauuaccgaa aguacugaga acuccaagau gaugcuggaa     1680

cuagauccac ccuuuggcga cagcuacaua gugaucggag uuggcgauaa gaaaauuaca     1740

caccauuggc acaggucugg gucaaccauc ggaaaggcuu ucgaggccac aguccggggu     1800

gcaaaacgca uggccgugcu cggcgacacu gcuugggauu ucgggagugu gggaggcguc     1860

uuuaacagcc uggguaaggg gauacaucag auuuucggcg ccgcauuuaa aucccuuuuc     1920

ggaggcaugu cuugguuuuc gcaaaucuug aucgggaccc ugcucguuug gcugggacuu     1980

aauacgaaga acggcagcau uucccugacc uguuuggcuc ucgguggggu gaugaucuuc     2040

cugucaacag ccgugagugc g                                               2061


<210>  1860
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short CDS opt

<400>  1860
accagcaucg gcauuguggg gcugcuccuu acaacugcca uggcugcaga gauaacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uaucucauuc      120

gccaccacau ugggcgucaa caaaugccac guucagauua uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguggaacc ugaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuacg gcacuuguca acacaagaaa      300

ggugaggccc gccgaucccg uagagcugug acacuaccau cucauucgac caggaagcuc      360

cagacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaccu uaucaagguu      420

gagaacugga uuuuuagaaa uccgggguuc gcacuggugg ccgucgcuau cgccugguug      480

cucggaaguu ccacaucuca gaaggugaua uaccugguaa ugauccugcu cauugcgccc      540

gcauauagca ucaggugcau uggcguuucc aaccgggacu uuguggaagg caugucaggg      600

ggaacuuggg ucgauguggu guuagagcau gguggcugug ucaccguuau ggcccaagac      660

aaaccuacag uggauaucga acugguaacc acgacuguga guaauauggc ugagguccgc      720

agcuacugcu acgaggccuc uauauccgac auggcaucag auagccgaug uccaacacag      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ugugcaaaag aacuuugguu      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcauuc aacccgaaaa ucucgaauau      960

cggaucaugc ugucggucca cgggucucag cacuccggaa ugauugugaa cgacaucggu     1020

caugagaccg augaaaaucg ugcgaaggua gagguuacac cuaacucacc acgcgccgag     1080

gcaacucuug gcggguucgg aagucugggc uuggacugug aacccagaac cggucucgau     1140

uuuagcgacc uguacuauuu aacaaugaau aacaaacacu ggcuggugca uaaggagugg     1200

uuccacgaua ucccucuccc auggcacgcu ggggccgaca cuggcacccc gcauuggaau     1260

aacaaagaag cucuugucga guuuaaggau gcccacgcaa aaaggcagac aguggucgug     1320

cugggauccc aggaaggcgc cguucauacc gcuuuggcgg gggcacuaga ggccgagaug     1380

gacggagcua agggucggcu guucucuggc caccucaagu gccgacugaa aauggauaaa     1440

cuucgccuga agggggugag cuacucauug uguacugccg uaugcacggc agccaagguc     1500

cccgcugaaa cacuccaugg aaccgugacu guugaggugc aauaugccgg caccgacggu     1560

ccuuguaaag ucccagcaca gauggcggug gacaugcaga cacugacccc cguagggaga     1620

uuaauuacug cuaauccugu gauaacagaa aguacggaga acuccaagau gaugcuggaa     1680

cuagauccac ccuuuggcga cagcuacauc guuauuggag ucggcgauaa gaaaaucacc     1740

caccacuggc auaggucugg gucaacuaua ggaaaggccu ucgaggcaac agugcggggu     1800

gccaaacgca uggcugugcu cggcgacacc gccugggauu uugggagugu cggaggcguu     1860

uucaacagcc uggguaaggg gauucaccaa aucuucggcg cagcuuuuaa aucccuuuuc     1920

ggaggcaugu cuugguuuuc gcagaucuug auugggacac ugcucgugug gcugggacuu     1980

aauacuaaga acggcagcau cucccugacc ugcuuggccc ucgguggggu gaugauuuuc     2040

cugucaacgg cgguaagugc c                                               2061


<210>  1861
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short CDS opt

<400>  1861
accagcaucg gcauuguggg gcugcuccuu acaacugcca uggcugcaga gauaacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uaucucauuc      120

gccaccacau ugggcgucaa caaaugccac guucagauua uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguggaacc ugaugacgua      240

gauuguuggu gcaauaccac aagcaccugg guggucuacg gcacuuguca ccauaagaaa      300

ggugaggccc gccgaucccg uagagcugug acacuaccau cucacucgac caggaagcuc      360

caaacgcggu cacagacuug gcuggaaagc cgcgaguaua ccaaacaucu uaucaagguu      420

gagaacugga uuuuuagaaa uccgggguuc gcacuggugg ccgucgcuau cgccugguug      480

cucggaaguu ccacaucuca gaaggugaua uaccugguaa ugauccugcu cauugcgccc      540

gcauauagca ucaggugcau uggcguuucc aaccgggacu uuguggaagg caugucaggg      600

ggaacuuggg ucgauguggu guuagagcac gguggcugug ucaccguuau ggcccaggac      660

aaaccuacag uggauaucga acugguaacc acgacuguga guaauauggc ugagguccgc      720

agcuacugcu acgaggccuc uauauccgac auggcaucag auagccgaug uccaacacaa      780

ggggaggcuu aucuugacaa gcagagugac acccaguacg ugugcaaaag aacuuugguu      840

gauaggggau ggggcaacgg uugugggcug uucggaaagg gcucucuagu gacaugcgcc      900

aaauuuaccu gcuccaagaa gaugacgggc aaaagcauuc agcccgaaaa ucucgaauau      960

cggaucaugc ugucggucca cgggucucaa cauuccggaa ugauugugaa cgacgagaau     1020

cgugcgaagg uagaaguuac cccuaacuca ccacgcgccg aggcaacacu ugguggcuuc     1080

gggagucugg gauuggauug ugagcccaga acuggccucg acuuuagcga ucuguacuau     1140

uuaaccauga auaacaaaca cuggcuggug cauaaggaau gguuccacga caucccucuc     1200

ccauggcacg cuggugccga uacagggacu ccgcauugga auaacaaaga ggcucuuguc     1260

gaauuuaagg acgcccacgc aaaaaggcag accguggucg ugcugggcuc ccaggaggga     1320

gccguucaua cagcuuuggc gggcgcacua gaagccgaga uggauggggc uaagggacgg     1380

cuguucucug gucaccucaa gugccgacug aaaauggaca aacuucgccu gaagggcgug     1440

agcuacucau uguguaccgc cgcauuuacu uucacgaagg uacccgccga gacacuccau     1500

gggaccguca cuguggaagu ucaguaugcu ggaaccgaug gcccuugcaa agugccagcc     1560

caaauggcag ucgacaugca gacacugacc cccgugggua gauuaaucac ugcgaauccu     1620

guaauuacag agaguacgga aaacuccaag augaugcugg agcuagaccc acccuuuggg     1680

gauagcuaca uagugaucgg cguuggagac aagaaaauua cccaccacug gcauaggucu     1740

ggcucaacua ucgggaaggc uuucgaagcc acaguccggg gagcaaaacg cauggccgug     1800

cucggugaua ccgcuuggga cuucggcagu gucgggggag uguuuaacag ccugggcaag     1860

gguauacacc agauuuucgg ggccgcauuu aaaucccuuu ucggcggaau gucuugguuu     1920

ucgcaaaucu ugaucggcac acugcucguu uggcuggggc uuaauacuaa gaacggaagc     1980

auuucccuga ccuguuuggc ucucggcggu gugaugaucu uccugucaac ggccgugagu     2040

gcg                                                                   2043


<210>  1862
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short CDS opt

<400>  1862
accagcaucg gcauuauagg gcugcuccuu acaacugcca uggcugcaga gaucacgaga       60

aggggauccg ccuacuauau guaccuggac cggucugaug cggguaaggc uauuucauuc      120

gccaccacau ugggcgugaa caaaugccac guccagauca uggacuuagg gcauaugugu      180

gaugcaacua ugaguuauga augccccaug cuggacgagg gaguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aagcaccugg guaguguacg gcacuuguca ccauaagaaa      300

ggugagacac gccgaucccg uagaucuguc ucgcuaaggu aucacuacac ccggaagcuc      360

caaacgcgcu cacagacuug gcuggaaagc agagaguaua aaaagcaucu uauuauggug      420

gagaacugga ucuuuaggaa uccaggguuc gccauaguua guguggcuau caccuggcug      480

augggauccu ugacaucuca gaaggucauu uaccucguga ugaucguacu gauuguuccg      540

gcauacagca ucuccugcau aggcguguca aaccgggacc uggucgaagg caugaguggg      600

ggaacuuggg uggauguggu ccucgagcac gguggcugug uuaccgaaau ggcccaggac      660

aaacccacag uggauauuga guuaguaacc augacgguga gcaauauggc ugagguccgc      720

ucuuauugcu acgaagccuc ccugucagac auggcgagcg caagucgaug uccuacucaa      780

ggggagccau cucuugauaa gcaguccgac acacagagcg ugugcaaaag aaccuuggga      840

gacaggggcu gggguaacgg guguggaauc uuuggcaagg gcucgcuggu uacuugcucu      900

aaauucacau gcuguaagaa gaugcccggg aaauccauuc agccugaaaa ucuagaguau      960

cggaucaugc ucccagugca cggaucacaa cauaguggua ugaucgucaa cgauauuggc     1020

cacgaaaccg acgagaaucg ugccaaggug gagguaacgc ccaacagccc ucgcgcugaa     1080

gccacccugg gaggguuugg cucccuuggu cuggauugcg agccaagaac aggguuggac     1140

uucucugauc ucuacuaucu gacuaugaau aacaaacauu gguuaguuca caaggaaugg     1200

uuucacgaca uaccgcugcc cuggcaugca ggcgcugaua ccggaacacc ucacuggaau     1260

aacaaagagg cccucgugga guucaaggac gcgcaugcca aaaggcagac ugucgugguc     1320

cuuggcagcc aggaaggggc agugcacacc gcucuggccg gagcuuugga ggccgaaaug     1380

gauggugcaa agggccggcu auuuucaggg caucugaagu gucgacucaa aauggacaag     1440

cugcgccuua aaggaguuag uuacucccug ugcacagccg cuuucaccuu uacuaaggug     1500

ccagcggaga cguugcacgg cacaguaacc gucgaagugc agagcgcagg uacugauggg     1560

cccuguaaag uuccugccca aauggcugug gacaugcaga cccucacacc agucggcaga     1620

cugaucaccg ccaaucccgu gauuacugag ucuacagaaa acucaaagau gauguuagag     1680

cuggacccgc cuuucggaga uaguuauauc guaauaggcg ugggggacaa gaaaauuacg     1740

caccauuggc acaggagcgg auccaccauc gguaaggcau uugaggccac uguucggggc     1800

gcuaaacgca uggccguccu aggggauaca gcaugggacu ucggaucugu gggcggugug     1860

uucaacucgc ucgggaaggg cauccaucag auuuuuggag cggcuuucaa aagccuguuu     1920

ggcgggaugu ccugguucuc acaaauccuu auuggaaccu ugcuggucug gcucggccug     1980

aauacaaaga acgguaguau aucucuuacu ugccuggccc ucgggggagu uaugaucuuu     2040

uugagcaccg caguguccgc c                                               2061


<210>  1863
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short CDS opt

<400>  1863
agcgugggca ucgucgggcu gcuccuuacc acagccaugg cugcagaggu uacuagaagg       60

ggauccgccu acuauaugua ccuggaccgg aacgaugcgg gugaagcuau uucuuucccc      120

acgaccuugg gcaugaauaa gugcuauaua cagaucaugg acuuagggca caugugugau      180

gccacaaugu cauacgagug cccuaugcug gacgaaggag uggagccaga ugacguagau      240

uguuggugca acacuaccag uacaugggug gucuauggca ccugucauca caaaaagggu      300

gaagcacgcc gaagccguag agccgugacu cuaccguccc auucuacaag gaaacuccaa      360

acccggucgc agacguggcu ggagucacgc gaguacacua agcaccuuau uagaguugaa      420

aauuggaucu uuaggaaccc cggguucgcu cuggcagccg cugccauugc gugguugcuc      480

ggaagcagua ccucccagaa agugaucuau cuggucauga uacugcucau cgccccugca      540

uacucuauuc ggugcaucgg cgugagcaau cgcgacuuug uagagggcau guccggggga      600

acauggguug auguggucuu agaacauggu ggcuguguga cugugauggc ucaggacaag      660

ccaaccgucg auauugagcu gguuacaacc acggugucaa acauggccga gguacgaagu      720

uacugcuaug aagcaagcau cucugacaug gcuuccgauu caagaugucc cacucaaggg      780

gaggccuacc uugacaagca gagcgacaca caguaugugu gcaaaaggac cuuggucgau      840

cggggauggg gcaaugguug ugggcuguuc ggaaagggca gucuagugac uugcgcgaaa      900

uuugccugcu cuaagaaaau gacaggcaag uccauacagc cugaaaaccu cgaguaccgu      960

auuaugcuga gcguucacgg gucgcaacac ucuggaauga ucgugaauga caccggucau     1020

gaaacggaug agaaccgcgc aaaggucgag auuaccccaa auucccccag agcugaagcc     1080

acacuuggcg gguucggauc acugggcuug gacugugagc cuaggacugg ucucgauuuu     1140

agugaccugu auuacuuaac caugaacaau aaacacuggc uggugcauaa ggaaugguuc     1200

cacgauaucc cacucccgug gcacgcuggg gccgacacag gcacucccca uuggaacaau     1260

aaagaggcac uuguagaauu uaaggaugcc cacgcuaaac ggcagaccgu ugugguccug     1320

ggaagccagg agggcgcggu gcauacagca uuggccgggg cucuagaggc cgaaauggac     1380

ggagcaaagg gucgacuguc cucuggccac cucaaaugcc gccugaagau ggauaaacuu     1440

agacugaagg gggucagcua uucauugugu accgccgcuu ucacuuuuac gaagaucccu     1500

gccgagacac uccauggaac cgugacuguu gaagugcagu acgcaggcac cgacggucca     1560

ugcaaaguac ccgcgcaaau ggcugucgac augcagacac ugaccccugu ggggagguua     1620

auuacugcca acccaguuau aacagagagu acggaaaacu ccaagaugau gcuggagcua     1680

gaucccccgu ucggcgacag cuacaucgug auuggagucg gcgagaaaaa gaucacccac     1740

cacuggcauc ggucuggguc aacuauagga aaggcauuug aagccacagu gcgcggugcu     1800

aaaagaaugg ccguacucgg cgauaccgca ugggacuucg ggaguguggg aggcgcucug     1860

aauagccuug guaaggggau ucaccagauc uucggcgccg cguuuaaauc cuuguucgga     1920

ggcaugucuu gguuuucgca aauccugauu gggacacucc ugauguggcu uggacugaac     1980

acuaagaaug gcagcaucuc cuugaugugu cucgcccugg gugggguuuu aauuuuccug     2040

ucaaccgcug ucagugca                                                   2058


<210>  1864
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short CDS opt

<400>  1864
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauauuga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagauuacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaauuacag ccaacccagu uauaacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacu     1740

caccacuggc aucggucugg gucaacaaua ggaaaggcau uugaagccac cgugcgcggu     1800

gcuagaagga uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaagg gauucaccag aucuucggcg ccgcguuuaa guccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacuc uccugaugug gcuuggacug     1980

aacaccaaaa auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1865
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short CDS opt

<400>  1865
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga uguuuaggaa ccccggguuc gcucuggcag ccgcugccau cgcgugguug      480

cucggaagca guacauccca gaaagugauu uaucugguca ugauccugcu cauagcaccc      540

gccuacucua uccggugcau uggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgugau ggcucaggac      660

aagccaacag ucgauaucga gcugguuacc acgacugugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag cauuucugac auggcuuccg auucaagaug ucccacacaa      780

ggggaggccu accuugacaa gcagagcgac acccaguaug ugugcaaaag gacuuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacaugcgcg      900

aaauuugccu gcucuaagaa aaugaccggc aaguccaucc agccugaaaa ccucgaguac      960

cguauaaugc ugagcguuca cgggucgcaa cacucuggaa ugauugugaa ugacacgggu     1020

caugaaaccg augagaaccg cgcaaagguc gagaucacac caaauucccc cagagcugaa     1080

gccacucuug gcggguucgg aucacugggc uuggacugug agccuaggac cggucucgau     1140

uuuagugacc uguauuacuu aacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua uuccacuccc guggcacgcu ggggccgaca cuggcacccc ucauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac aguugugguc     1320

cugggaagcc aggagggcgc ggugcauacc gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguacugccg cuuucacguu uacaaagauc     1500

ccugccgaga cccuccaugg aacugugacc guugaagugc aguacgcagg cacagacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cccugacucc uguggggagg     1620

uuaaucacag ccaacccagu uauuacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacaua gugaucggag ucggcgagaa aaagauuacu     1740

caccacuggc aucggucugg gucaacaauc ggaaaggcau uugaagccac cgugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauaca gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauacaccag auuuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug aucgggacuc uccugaugug gcuuggacug     1980

aacaccaaga auggcagcau uuccuugaug ugucucgccc uggguggggu uuuaaucuuc     2040

cugucaacgg cugucagugc a                                               2061


<210>  1866
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short CDS opt

<400>  1866
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaugac caguacaugg guggucuaug gcaccuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acucuaccgu cccauucuac aaggaaacuc      360

caaacccggu cgcagacgug gcuggaguca cgcgaguaca cuaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guaccuccca gaaagugauc uaucugguca ugauacugcu caucgccccu      540

gcauacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacauggg uugauguggu cuuagaacau gguggcugug ugacugugau ggcucaggac      660

aagccaaccg ucgauauuga gcugguuaca accacggugu caaacauggc cgagguacga      720

aguuacugcu augaagcaag caucucugac auggcuuccg auucaagaug ucccacucaa      780

ggggaggccu accuugacaa gcagagcgac acacaguaug ugugcaaaag gaccuugguc      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu gacuugcgcg      900

aaauuugccu gcucuaagaa aaugacaggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcguuca cgggucgcaa cacucuggaa ugaucgugaa ugacaccggu     1020

caugaaacgg augagaaccg cgcaaagguc gagauuaccc caaauucccc cagagcugaa     1080

gccacacuug gcggguucgg aucacugggc uuggacugug agccuaggac uggucucgau     1140

uuuagugacc uguauuacuu aaccaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccacuccc guggcacgcu ggggccgaca caggcacucc ccauuggaac     1260

aauaaagagg cacuuguaga auuuaaggau gcccacgcua aacggcagac cguugugguc     1320

cugggaagcc aggagggcgc ggugcauaca gcauuggccg gggcucuaga ggccgaaaug     1380

gacggagcaa agggucgacu guccucuggc caccucaaau gccgccugaa gauggauaaa     1440

cuuagacuga agggggucag cuauucauug uguaccgccg cuuucacuuu uacgaagauc     1500

ccugccgaga cacuccaugg aaccgugacu guugaagugc aguacgcagg caccgacggu     1560

ccaugcaaag uacccgcgca aauggcuguc gacaugcaga cacugacccc uguggggagg     1620

uuaauuacug ccaacccagu uauaacagag aguacggaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacauc gugauuggag ucggcgagaa aaagaucacc     1740

caccacuggc aucggucugg gucaacuaua ggaaaggcau uugaagccac agugcgcggu     1800

gcuaaaagaa uggccguacu cggcgauacc gcaugggacu ucgggagugu gggaggcgcu     1860

cugaauagcc uugguaaggg gauucaccag aucuucggcg ccgcguuuaa auccuuguuc     1920

ggaggcaugu cuugguuuuc gcaaauccug auugggacac uccugaugug gcuuggacug     1980

aacacuaaga auggcagcau cuccuugaug ugucucgccc uggguggggu uuuaauuuuc     2040

cugucaaccg cugucagugc a                                               2061


<210>  1867
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short CDS opt

<400>  1867
accagcgugg gcaucgucgg gcugcuccuu acaacugcca uggcugcaga gguuacgaga       60

aggggauccg ccuacuauau guaccuggac cggaacgaug cgggugaagc uauuucuuuc      120

cccaccacau ugggcaugaa uaagugcuau auacagauca uggacuuagg gcacaugugu      180

gaugccacua ugucauacga gugcccuaug cuggacgaag gaguggagcc agaugacgua      240

gauuguuggu gcaacaccac aaguaccugg guggucuaug gcacuuguca ucacaaaaag      300

ggugaagcac gccgaagccg uagagccgug acacuaccgu cccauucuac caggaaacuc      360

caaacgcggu cgcagacuug gcuggaguca cgcgaguaca ccaagcaccu uauuagaguu      420

gaaaauugga ucuuuaggaa ccccggguuc gcucuggcag ccgcugccau ugcgugguug      480

cucggaagca guacauccca gaaagugauc uaucugguca ugauacugcu caucgcaccc      540

gccuacucua uucggugcau cggcgugagc aaucgcgacu uuguagaggg cauguccggg      600

ggaacuuggg uugauguggu cuuagaacau gguggcugug ugaccgcuau ggcccaggac      660

aagccaacag uggauauuga gcuggucacc acgacuguuu caaacauggc agaggugcga      720

aguuacugcu augaagcuag caucucugac auggccuccg auucaagaug ucccacacaa      780

ggggaggcgu accuugacaa gcagagcgac acccaguaug uaugcaaaag gacuuuggug      840

gaucggggau ggggcaaugg uugugggcug uucggaaagg gcagucuagu cacaugcgcc      900

aaauuugcau gcucuaagaa aaugaccggc aaguccauac agccugaaaa ccucgaguac      960

cguauuaugc ugagcgugca cgggucgcaa cacucuggaa ugcuuguuaa ugacacgggu     1020

caugaaaccg augagaaccg cgcuaaggug gagaucacac caaauucccc cagagccgaa     1080

gcuacucugg gcggguucgg aucauugggc cucgacugug agccuaggac cggucuggau     1140

uuuagugacu uauauuaccu gacaaugaac aauaaacacu ggcucgccca uaaggaaugg     1200

uuccacgaua uuccacuucc guggcacgca ggggccgcua cuggcacccc ucauuggaac     1260

aauaaagagg cgcuggucga auuuaaggac gcacacgcca aacggcagac agugguaguu     1320

uugggaagcc aggagggcgc ugugcauacc gcccuagcag gggcccugga ggcugaaaug     1380

gauggagcca agggucgacu cuccucuggc caccugaaau gccgccuuaa gauggacaag     1440

cugagauuga aaggggucag cuauucacuc uguacugcag cguucacguu uacaaagauc     1500

ccugcugaga ccguggaugg aacugucacc guggaaggcc aguacggugg gacagacggc     1560

ccaugcaaag uucccgccca aauggcagug gacaugcaga cccugacucc uguaggaagg     1620

uuaaucacag ccaacccagu cauuacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagaucccc cguucggcga cagcuacaua gugaucgggg uuggagagaa aaagauuacu     1740

caucacuggc accggucugg uucaacaauc ggcaaggcuu uugaagccac cgugcgcggg     1800

gcaaaaagaa uggcuguccu cggagauaca gccugggacu ucggcagugu ggguggggcg     1860

cugaauagcc uuggcaaggg aauacaucag auuaucggcg ccgcuuucaa auccuuguuu     1920

gggggaaugu cuugguucuc gcaaauccug auuggcacuc uccugaugug gcuuggucug     1980

aacaccaaga augggagcau cuccuugaug ugucucgcac ugggaggcgu auuaauuuuu     2040

cugucaacgg ccgugagugg u                                               2061


<210>  1868
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short CDS opt

<400>  1868
ggcgccgacg cuagcaucgg gauugugucc cugcuccuua ccacagucau ggcagccgag       60

auaacuagaa ggggaucugc guacuauaug uaccuggauc ggucagacgc ugguaaggcc      120

aucaguuucg uuacgaccuu gggcgugaac aaaugccacg uacagauuau ggauuuaggg      180

cauaugugug acgcaacaau gagcuaugaa ugccccaugc uggaugaggg aguggaaccu      240

gacgaugucg acuguuggug caauacuacc uccacauggg ugguuuacgg caccugucac      300

cauaagaaag gugaggcccg ccgaucucgu agagcuguga cucuaccauc gcacucaaca      360

aggaagcucc aaacccggag ccagacgugg cuggaaaguc gcgaguauac uaaacaucuu      420

aucaaggucg agaacuggau uuuuagaaau ccgggguucg cacugguggc cguagcuauc      480

gccugguugc ucggauccuc uaccagccag aagguuauau accuggugau gauccugcuc      540

auugcgcccg cauauuccau caggugcauu ggcgucucaa accgggauuu uguggaaggc      600

augagugggg gaacaugggu ggacgucguu uuagagcacg guggcugugu gacuguaaug      660

gcccaggaua aaccuaccgu ggacaucgaa cuggucacaa ccacggugag caauauggcu      720

gagguucgcu cuuacugcua cgaggccucc auaucagaua uggcaagcga cagucgaugu      780

ccaacucaag gggaggcuua ucuugacaag cagucugaua cacaguacgu gugcaaaaga      840

accuuggucg acaggggaug gggcaacggu ugugggcugu ucggaaaggg cucccuagug      900

acuugcgcca aauuuacaug cagcaagaag augaccggca aaucgauuca gcccgaaaau      960

cucgaauauc ggaucaugcu gucuguacac gggucccaac auucaggaau gauuguuaac     1020

gaugagaauc gugcgaaggu ggaagucacg ccuaacaguc cacgcgccga ggcaacccuu     1080

gguggcuucg ggagccuggg auuggacugu gagcccagaa caggccucga uuuuuccgac     1140

cuguacuauu uaacuaugaa uaacaaacac uggcuggugc auaaggaaug guuccacgau     1200

aucccucucc cauggcacuc uggugcugac accgagacac cgcauuggaa uaacaaagaa     1260

gcccuugucg aguuuaagga ugcucacgcc aaaaggcaga cugugguugu gcuggggagc     1320

caggaaggcg caguacauac cgccuuggcu ggagcgcuag aggcagagau ggacggcgcc     1380

aaggggcggc ugucaagugg acaccucaag ugccgacuga aaauggauaa acuucgccug     1440

aagggugucu ccuacagcuu guguacagcu gccuucaccu uuacuaaggu gcccgcagaa     1500

acgcuccaug gcacaguuac cguggagguc caguaugccg ggagagacgg accuugcaaa     1560

gugccagcuc aaauggccgu agacaugcag acucugaccc ccgugggcag guuaaucaca     1620

gcaaauccug uuauuaccga aucuacugag aacucaaaga ugaugcugga acuagaucca     1680

cccuucggug acaguuacau agucaucggg gugggcgaua agaaaauuac acaccacugg     1740

caucggagcg gauccaucau aggcaaggcg uuugaggcua cggugcgcgg ggccaaaaga     1800

auggcagucc ucggagacac cgccugggau uucgguucug uuggcggggu guucaacucg     1860

cugggaaagg gcauucacca gaucuuuggu gcugccuuca aaagccuuuu ugggggcaug     1920

uccugguucu cacaaaucuu gauuggaacu cugcucgugu ggcugggccu uaauacaaag     1980

aacgggagua ucucucugac cuguuuggca cucggaggcg uaaugauuuu ucugagcaca     2040

gcugucuccg cc                                                         2052


<210>  1869
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short CDS opt

<400>  1869
ggcgccgaca ccagcaucgg gauuauagga cugcuccuua caacugcuau ggcagccgag       60

aucacgagaa gggguuccgc guacuauaug uaccuggauc ggucugacgc uggcaaggcc      120

auuucauucg caaccacaau gggggugaac aaaugccacg uccagaucau ggauuuggga      180

cauaugugug acgccacuau gaguuaugaa ugccccaugu uagaugaggg cguugaaccu      240

gacgaugugg acuguuggug caauaccaca agcaccuggg uaguguacgg uacuugucac      300

cauaagaaag gggaggcucg cccaucccga cgugcaguca cacugccguc ucacucgacc      360

agaaagcuac aaacgagguc acagacuugg cucgaaagcc gggaguauac caaacaucug      420

auuaaggugg agaacuggau ccuucgcaau cccggauuua caguugccgc ucuggccaug      480

gcgugguugc ucggcaacag uacuucccag agagugauau accuggucau gauccugcuc      540

auugcacccg ccuauucuau caggugcauu ggcgugagca aucgggauuu cguagaaggg      600

auguccggag guaccugggu ugacgugguc uuagagcacg gcgggugugu gacagugaug      660

gcucaggaua agccaaccgu cgacaucgaa cugguuacga cuacaguguc aaacauggcc      720

gagguacgca guuacugcua cgaggcaagc auaucugaua uggcuuccga cucacgaugu      780

cccacccaag gagaagccua ucuugacaaa cagagcgaua cucaguacgu gugcaagaga      840

acauuggucg acaggggcug ggguaauggg uguggacugu uuggcaaagg cagucuagug      900

accugcgcga aguucacgug cucuaaaaag augaccggga aguccauuca gccugagaac      960

cucgaauauc ggaucaugcu gagcguucac ggaucgcaac auucugguau gauugugaau     1020

gauaucggcc acgagacaga cgaaaaccgu gccaaagucg aggugacucc aaauuccccu     1080

cgcgcagagg cuacccuugg gggauuuggc ucacuggguu uggauuguga accuagaaca     1140

gggcucgacu ucagugaucu guacuauuua acuaugaaca auaagcauug gcugguacac     1200

aaagaguggu uucacgacau cccacucccg uggcaugccg gcgcugauac cggaacaccc     1260

cacuggaaca auaaggaagc ccuuguugag uucaaagacg cacaugccaa gaggcagacc     1320

guggucgugc ugggcagcca ggaaggggcu guccacacug cguuggcagg agcccuagag     1380

gcugagaugg auggugccaa aggccggcug uuuuccgggc aucucaagug ccgaauuaag     1440

auggacaaac ugcgccuuaa gggagugucu uacagccugu guacggcagc cuucacauuu     1500

ucaaaaguuc cugcugaaac cuugcacggc acugugaccg uagaggucca guaugccggu     1560

agagacgggc caugcaaggu gcccgcacaa auggcgguug auaugcagac acucaccccu     1620

gugggcaggc ugauaacugc uaacccaauc auuacagaaa guacggagaa cucccggaug     1680

auguuagaac uggauccccc guucggagac agcuacaucg ucauaggcgu gggggacaaa     1740

aagauuaccc accauuggca ccgcucugga ucaacuaucg guaaggccuu ugaggcaaca     1800

guaagaggcg ccaaaaggau ggcugugcua ggggauaccg ccugggacuu cggaaguguu     1860

ggcggugucu ucaauagccu cgggaagggc gugcaucagg uguuuggagc agcuuucaaa     1920

ucccuguuug gcgggauguc uugguucucg caaauccuua uuggaacauu gcuggucugg     1980

cucggccuga acacuaagaa ugguagcauc ucccuugccu gucuggcguu gggaggggcc     2040

augauuuuuc ucucaaccgc uguuagugca                                      2070


<210>  1870
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE CDS opt

<400>  1870
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

aucgugcugg aacacggcgg gugugucaca gugauggcac aagacaaacc uacuguugau      180

auugaguugg ugacgaccac agucaguaau auggccgaag ugcggagcua cugcuaugag      240

gcguccaucu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguaugc aaacgaacac ugguugaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauacaaccg gagaaucugg aguacagaau caugcuuagc      480

guccauggau cccagcacuc agguaugauu gugaacgaua caggccauga aaccgacgag      540

aauagggcua agguggaaau cacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guccauaagg aaugguuuca cgacauuccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guugaguuca aggaugcaca cgccaagaga cagacagugg uaguguuggg cucccaggaa      840

ggggcugucc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugaguuacu cucucugcac ggccgcauuu acuuucacaa agauccccgc ugagacccug     1020

cacggcacug uuacagugga aguccaauau gccgguaccg acgggccuug uaaagugcca     1080

gcucagaugg ccguagauau gcagacguua accccgguug gccgacugau aacagcaaau     1140

cccgugauua cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acaucgucau uggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguca ggggcgcgaa acggauggca     1320

gugcuggggg acacagccug ggauuuugga uca                                  1353


<210>  1871
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE CDS opt

<400>  1871
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guucuggggg acacagccug ggauuuugga uca                                  1353


<210>  1872
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE CDS opt

<400>  1872
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc ggcuaugaaa ccgaugagga cagggccaag      540

guggaaguca cgcccaacag uccucgggcu gaggcaacuc ugggaggguu uggcagcuug      600

ggucucgauu gcgagccacg caccgggcug gacuucucug aucuguacua ccucacaaug      660

aauaacaaac auugguuagu ucacaaggaa ugguuucaug acauuccccu gccuuggcac      720

gccggagcug acacuggcac cccacacugg aauaacaaag aggcccuugu ggaguucaag      780

gaugcgcaug caaagagaca gacaguagug gucuugggcu cccaggaagg ggccgugcac      840

accgcucugg ccggagcacu agaagcugag auggacggug ccaaaggcag gcucuuuuca      900

gggcaucuga agugucggcu uaaaauggau aagcugcgcu ugaaaggagu uagcuauagu      960

cucugcacgg cggccuucac uuuuacaaag gugcccgcag agacccugca cggcacuguc     1020

acaguggaag uacaauacgc ugguaccgac gggccuugua aaaucccagu ucagauggcc     1080

guggauaugc agacguuaac cccggucggc cgacugauua cagcuaaucc cgugaucacu     1140

gagucuaccg aaaacuccaa gaugaugcuc gagcuugacc cuccauucgg agauagcuau     1200

auagucauug gcguggggga caagaaaauc acacaccauu ggcacagauc gggaucuacu     1260

auugguaagg ccuuugaagc aaccguuagg ggcgccaaac ggauggcugu gcugggggau     1320

acagcguggg acuucggauc c                                               1341


<210>  1873
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE CDS opt

<400>  1873
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guucuggggg acacagccug ggauuuugga uca                                  1353


<210>  1874
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE CDS opt

<400>  1874
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua ccggcuauga aacggacgag      540

aauagggcca aggucgaagu uacucccaac aguccucggg cugaggcaac ccugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcacagggc uggacuucuc ugaucuguac      660

uaccucacua ugaauaacaa acauugguua gugcacaagg aaugguuuca ugacauuccc      720

cugccuuggc acgccggagc ugacaccggc acaccacacu ggaauaacaa agaggcccuu      780

guagaguuca aggaugcgca ugcaaagaga cagaccgugg ucguguuggg cucccaggaa      840

ggggccguuc acacggcucu ggccggagca cuagaagcug agauggacgg ugccaaaggc      900

aagcucuuuu cagggcaucu gaaauguagg cuuaagaugg auaaacugcg guugaaggga      960

gugagcuaua gucucugcac ugcggccuuc acauuuacca aaguccccgc agagacucug     1020

cacggcacag ugaccguaga aguucaauac gcugguacgg acgggccuug uaagauccca     1080

gugcagaugg ccgucgauau gcagaccuua acaccggugg gccgccugau uacugcuaau     1140

cccgucauca ccgagucuac agaaaacucc aagaugaugc ucgagcuuga cccuccauuc     1200

ggagauagcu auauagugau uggcguuggg gacaaaaaga ucacucacca uuggcaccga     1260

ucgggaucua ccauugguaa agccuuugaa gcaacaguga gaggcgccaa gaggauggcu     1320

guacuggggg auaccgcgug ggacuucgga ucc                                  1353


<210>  1875
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE CDS opt

<400>  1875
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugacc gugaacgaua ucggcuauga aacggacgag      540

aauagggcca aggucgaagu uacucccaac aguccucggg cugaggcaac ccugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcacagggc uggacuucuc ugaucuguac      660

uaccucacua ugaauaacaa acauugguua gugcacaagg aaugguuuca ugacauuccc      720

cugccuuggc acgccggagc ugacaccggc acaccacacu ggaauaacaa agaggcccuu      780

guagaguuca aggaugcgca ugcaaagaga cagaccgugg ucguguuggg cucccaggaa      840

ggggccguuc acacggcucu ggccggagca cuagaagcug agauggacgg ugccaaaggc      900

aagcucuuuu cagggcaucu gaaauguagg cuuaagaugg auaaacugcg guugaaggga      960

gugagcuaua gucucugcac ugcggccuuc acauuuacca aaguccccgc agagacucug     1020

cacggcacag ugaccguaga aguucaauac gcugguacgg acgggccuug uaagauccca     1080

gugcagaugg ccgucgauau gcagaccuua acaccggugg gccgccugau uacugcuaau     1140

cccgucauca ccgagucuac agaaaacucc aagaugaugc ucgagcuuga cccuccauuc     1200

ggagauagcu auauagugau uggcguuggg gacaaaaaga ucacucacca uuggcaccga     1260

ucgggaucua ccauugguaa agccuuugaa gcaacaguga gaggcgccaa gaggauggcu     1320

guacuggggg auaccgcgug ggacuucgga ucc                                  1353


<210>  1876
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE CDS opt

<400>  1876
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaug aaaauagggc caaggucgag      540

guuaccccca acaguccucg ggcugaagca acgcugggcg gguuuggcag cuugggacuc      600

gacugcgagc cacgcacugg ucuggauuuc ucugaccugu auuaccucac caugaauaac      660

aaacauuggu uagugcacaa ggagugguuu caugauauuc cccugccuug gcacgccggg      720

gcugacacag gaacuccaca cuggaauaac aaagaagccc uuguagaguu caaggacgcg      780

caugcaaaga gacagaccgu ggucguguug ggcucccagg aaggcgccgu ucacacagcu      840

cuggccgggg cacuagaggc ugaaauggau ggagccaaag guaggcucuu uucaggccau      900

cugaaguguc ggcuuaaaau ggacaagcug cgcuugaaag gggugagcua cagucucugc      960

accgcggccu ucacguuuac uaaggucccc gcagagacac ugcacggaac cgugacugua     1020

gagguucaau augcuggcac agaugguccu uguaaagugc cagcccagau ggcugucgac     1080

augcagaccu uaacgccggu ggggcgacug aucaccgcca aucccgucau uacagaaucu     1140

acugagaacu ccaagaugau gcucgaacuu gauccuccau ucggcgacag cuacaucgug     1200

auaggaguug gcgauaagaa aauuacccac cauuggcaca gaucgggguc uacaaucgga     1260

aaggcauuug aggccacugu gaggggugcu aaacggaugg cgguacuggg cgacaccgca     1320

ugggauuucg ggucc                                                      1335


<210>  1877
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE CDS opt

<400>  1877
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc ggcuaugaaa ccgaugagaa cagggccaag      540

guggaaguca cgcccaauag uccucgggcu gaggcaacuc ugggaggguu uggcagcuug      600

ggucucgacu gcgagccacg caccgggcug gauuucucug accuguacua ccucacaaug      660

aacaauaaac auugguuagu ucacaaggaa ugguuucaug auauuccccu gccuuggcac      720

gccggagcug acacuggcac cccacacugg aacaauaaag aggcccuugu ggaguucaag      780

gacgcgcaug caaagagaca gacaguagug gucuugggcu cccaggaagg ggccgugcac      840

accgcucugg ccggagcacu agaagcugag auggauggug ccaaaggcaa gcucuuuuca      900

gggcaucuga aauguaggcu uaagauggac aaacugcggu ugaagggagu uagcuauagu      960

cucugcacgg cggccuucac uuuuacaaaa gugcccgcag agacccugca cggcacuguc     1020

acaguggaag uacaauacgc ugguaccgau gggccuugua agaucccagu ucagauggcc     1080

guggacaugc agacguuaac cccggucggc cgccugauua cagcuaaccc cgugaucacu     1140

gagucuaccg aaaauuccaa gaugaugcuc gagcuugauc cuccauucgg agacagcuau     1200

auagucauug gcguggggga uaaaaagauc acacaccauu ggcaccgauc gggaucuacu     1260

auugguaaag ccuuugaagc aaccguuaga ggcgccaaga ggauggcugu gcugggggac     1320

acagcguggg auuucggauc c                                               1341


<210>  1878
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE CDS opt

<400>  1878
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uucccugaga       60

ugcaucggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

guggucuugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

auugaguuag ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccaucu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuaccug      300

gacaagcaga gcgauaccca guauguuugc aaacgaacau ugguggaccg uggcuggggu      360

aacgggugug gacucuuugg caagggcagu cuggugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauucaaccg gagaaucuug aguacagaau caugcugagc      480

guccauggau cccagcacuc agguaugaua gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau cacgcccaac aguccucggg cagaggccac ucuaggggga      600

uuuggcagcc ucggucugga uugcgagcca cgcaccgggc uggacuucuc ugaucucuau      660

uacuuaacaa ugaauaacaa acacuggcug guucauaagg aaugguuuca cgacauuccc      720

cuuccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcguug      780

guggaguuca aggaugcaca cgccaagaga cagacaguag ugguccuggg cucccaggaa      840

ggggcugugc auaccgcccu agcaggagcu cucgaagccg agauggacgg ugcgaaaggc      900

aggcugucaa gcgggcaccu uaagugucgg cugaaaaugg auaaguugcg ccucaaagga      960

guuaguuacu cucugugcac ggccgcauuu acuuucacaa agauccccgc ugagaccuua     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacgcug accccggugg gccgacucau uacagcaaau     1140

cccgucauca cugaguccac cgaaaacagc aagaugaugc uugagcugga cccuccauuu     1200

ggagauucgu acauagugau uggcgucggg gaaaagaaaa ucacacauca cuggcauaga     1260

ucuggaucca cuauugguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guuuuggggg acacagccug ggauuuugga uca                                  1353


<210>  1879
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE CDS opt

<400>  1879
agcaagaaag ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aggacaagcc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucaaggaga agccuacuua      300

gacaaacaga gcgauaccca guauguuugc aagcgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caaaggcagu cucgugaccu gcgcaaaguu cgccuguucc      420

aagaaaauga cugggaaguc uauccagccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccaacacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aagucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa gcacugguua guucauaaag aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa ggaggcgcuu      780

guggaguuca aggaugcaca cgccaaaaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaagggc      900

aggcucucaa gcgggcaccu gaaaugucgg cuuaagaugg auaaacugcg cuugaaggga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa aaauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaguau gccgguaccg acgggccuug uaagguacca     1080

gcucaaaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gagaaaaaga uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa agccuucgaa gcuaccguga ggggcgcgaa gcggauggca     1320

guucuggggg acacagccug ggauuuugga uca                                  1353


<210>  1880
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE CDS opt

<400>  1880
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacacauca cugguauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guucuggggg acacagccug ggauuuugga uca                                  1353


<210>  1881
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE CDS opt

<400>  1881
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aagcucucaa gcgggcaccu gaaauguagg cuuaagaugg auaaacugcg guugaaggga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa aaauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaagguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgccugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gagaaaaaga uuacacauca cuggcaucga     1260

ucuggaucca cuaucgguaa agccuucgaa gcuaccguga gaggcgcgaa gaggauggca     1320

guucuggggg acacagccug ggauuuugga uca                                  1353


<210>  1882
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE CDS opt

<400>  1882
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauac gcagaccuua acaccggugg gccgacugau cacugcaaau     1140

cccgucauaa ccgaguccac agaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacucauca cuggcauaga     1260

ucuggaucca ccaucgguaa ggccuucgag gcuacaguga ggggcgcgaa acggauggca     1320

guucuggggg acaccgccug ggauuuugga uca                                  1353


<210>  1883
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE CDS opt

<400>  1883
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaaa uucggagcua cugcuaugag      240

gcguccaucu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguaugc aaacgaacac ugguugaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauacaaccg gagaaucugg aguacagaau caugcuuagc      480

guccauggau cccagcacuc agguaugauu gugaacgaua caggccauga aaccgacgag      540

aauagggcua agguggaaau cacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guccauaagg aaugguuuca cgacauuccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guugaguuca aggaugcaca cgccaagaga cagacagugg uaguguuggg cucccaggaa      840

ggggcugucc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugaguuacu cucucugcac ggccgcauuu acuuucacaa agauccccgc ugagacccug     1020

cacggcacug uuacagugga aguccaauau gccgguaccg acgggccuug uaaagugcca     1080

gcucagaugg ccguagauau gcagacguua accccgguug gccgacugau aacagcaaau     1140

cccgugauua cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acaucgucau uggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguca ggggcgcgaa acggauggca     1320

gugcuggggg acacagccug ggauuuugga uca                                  1353


<210>  1884
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE CDS opt

<400>  1884
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgcg gcguauggca     1320

guucuggggg acacagccug ggauuuugga uca                                  1353


<210>  1885
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE CDS opt

<400>  1885
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggu uucggauuca cgcuguccaa cucagggaga ggcuuacuua      300

gacaagcaga gcgauaccca guaugugugc aaacgaacac uggucgaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgccaaauu cgcauguucc      420

aagaagauga cugggaaauc uauccaaccg gaaaaucugg aguacagaau aaugcuuagc      480

gugcauggau cccagcacuc agguaugauc gucaacgaua caggccauga aaccgacgag      540

aauagggcca agguugaaau uacgcccaac aguccucggg cugaggcaac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua gugcauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgccggagc ugacacuggc accccacauu ggaauaacaa agaggcccuu      780

guagaguuca aggaugcgca cgcaaagaga cagacagugg ucguguuggg cucccaggaa      840

ggggccguuc auaccgcucu ggccggagca cuagaagcug agauggacgg ugccaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugaguuacu cucucugcac ggcggccuuu acuuucacaa agauucccgc agagacccug     1020

cacggcacug ucacagugga aguacaauau gcugguaccg acgggccuug uaaaguucca     1080

gcccagaugg cuguggauau gcagacguua accccggucg gccgacugau cacagccaau     1140

cccgugauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugucau cggcgugggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggcauucgag gccaccguua ggggcgcuaa acggauggcg     1320

gugcuggggg acacagcaug ggauuuugga uca                                  1353


<210>  1886
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE CDS opt

<400>  1886
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccau ugaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acaucgugau uggcgucggg gaaaagaaaa ucacccauca cuggcauaga     1260

ucuggaucca caaucgguaa ggccuucgag gcuacuguga ggggcgcgaa acggauggca     1320

guucuggggg acaccgccug ggauuuugga uca                                  1353


<210>  1887
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE CDS opt

<400>  1887
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcauuggg gaaaagaaaa ucacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguca ggggcgcgaa acggauggca     1320

gugcuggggg acacagccug ggauuuugga uca                                  1353


<210>  1888
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE CDS opt

<400>  1888
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccuggcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

guggucuugg aacacggcgg gugugugaca guuauggcac aagacaaacc cacuguggau      180

aucgaguuag ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccua cucagggaga agccuaccug      300

gacaagcaga gcgauaccca guauguuugc aaacgaacau ugguggaccg uggcuggggu      360

aacgggugug gacucuuugg caagggcagu cuggugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaacca gagaaucuug aguacagaau aaugcugagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgccgaac aguccccggg cagaggccac ucuaggggga      600

uuuggcagcc ucggucugga uugcgagccu cgcaccgggc uggacuucuc ugaucucuau      660

uacuuaacaa ugaauaacaa acacuggcug guucauaagg aaugguuuca cgacauccca      720

cuucccuggc acgcuggagc cgacacuggc accccucauu ggaauaacaa agaggcguug      780

guggaguuca aggaugcaca cgccaagaga cagacaguag ugguccuggg cucccaggaa      840

ggggcugugc auaccgcccu agcaggagcu cucgaagccg agauggacgg ugcgaaaggc      900

aggcugucaa gcgggcaccu uaagugucgg cugaaaaugg auaaguugcg ccucaaagga      960

guuaguuacu cucugugcac ggccgcauuu acuuucacaa agauuccagc ugagaccuua     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggcccug uaaaguaccu     1080

gcucagaugg ccguugauau gcagacgcug accccagugg gccgacucau cacagcaaau     1140

ccggucauaa cugaguccac cgaaaacagc aagaugaugc uugagcugga ucccccuuuu     1200

ggagacucgu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guuuuggggg acacagccug ggauuuugga uca                                  1353


<210>  1889
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE CDS opt

<400>  1889
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cacccaggaa      840

ggggcugugc auacggcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucuccu cagggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuagcuaca gucucugcac ugccgcauuu acauucacca agauucccgc ugagacucug     1020

cacggcacag ugaccgucga agugcaauau gccgguacgg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagaccuua acaccggugg gccgacugau cacugcaaau     1140

cccgucauaa ccgagucuac agaaaacucc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauagcu acauugugau cggcgucggg gaaaagaaaa uuacucauca cuggcauaga     1260

ucgggaucua ccaucgguaa ggccuucgag gcuacaguga ggggcgcgaa acggauggca     1320

guucuggggg acaccgccug ggauuuugga ucc                                  1353


<210>  1890
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE CDS opt

<400>  1890
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguauua     1080

gcucagaugg ccguugauau gcagacgcug accccagugg gccgacucau cacagcaaau     1140

ccggucauaa cugaguccac cgaaaacagc aagaugaugc uugagcugga ucccccuuuu     1200

ggagacucgu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guuuuggggg acacagccug ggauuuugga uca                                  1353


<210>  1891
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE CDS opt

<400>  1891
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaagcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaggccg agauggaugg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg acaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugaaacccug     1020

cacggcacug ugacagucga ggugcaauau gccgguaccg augggccuug uaaaguacca     1080

gcucagaugg ccguugacau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaauccac cgagaacagc aagaugaugc ucgaacuuga uccuccauuu     1200

ggagacucgu acauugugau cggcgucggg gagaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guucuggggg auacagccug ggacuuugga uca                                  1353


<210>  1892
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE CDS opt

<400>  1892
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccgc ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcacugggc uggacuucuc ugaucuguau      660

uaccucacca ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgccggagc ggacacaggc acuccacauu ggaauaacaa agaggcacuu      780

guggaguuca aggaugccca cgcuaagaga cagaccguag uggucuuggg cucccaggaa      840

ggggccgugc auacagcacu ggcuggagcc cuagaagcgg agauggacgg ugccaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac cgcagcuuuu acguucacua agauucccgc cgagacacug     1020

cacggcaccg ugacugucga agugcaauau gcugguacag acgggccuug uaaaguacca     1080

gcccagaugg caguugauau gcagaccuua acgccggugg gccgacugau caccgccaau     1140

cccgucauaa cagaguccac ugaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

ucuggaucca caaucgguaa ggcuuucgag gcgacuguga ggggcgcaaa acggauggcc     1320

guucuggggg acaccgcuug ggauuuugga uca                                  1353


<210>  1893
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE CDS opt

<400>  1893
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucaguacgac cgugagcaau auggccgaag uacgguccua cugcuaugag      240

gcgacaauuu cugacaucgc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauacaaccg gagaaucugg aguacagaau caugcuuagc      480

guccauggau cccagcacuc agguaugauu gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau cacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacauuccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacagcug uaguguuggg cucccaggaa      840

ggggccgucc auaccgcacu ggcuggagcc cuagaagcgg agauggacgg ugccaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugaguuacu cucucugcac ggcagcuuuu acuuucacaa agauccccgc cgagacccug     1020

cacggcacug uuacagugga aguccaauau gcugguaccg acgggccuug uaaagugcca     1080

gcccagaugg caguagauau gcagacguua accccgguug gccgacugau aacagccaau     1140

cccgugauua cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acaucgucau uggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggcuuucgag gcgaccguca ggggcgcaaa acggauggcc     1320

gugcuggggg acacagcuug ggauuuugga uca                                  1353


<210>  1894
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE CDS opt

<400>  1894
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucucca agauucccgc ugagacacug     1020

cacggcaccg ugacugaaga gguccaauau gccgguacag acgggccuug uaaagugcca     1080

gcucagaugg ccguagauau gcagaccuua acgccgguug gccgacugau caccgcaaau     1140

cccgugauaa cagaaagcac ugagaacucg aagaugaugc ucgaacuuga cccuccauuu     1200

ggagauucuu acauugucau cggcgugggg gagaagaaaa uuacccauca cuggcauaga     1260

uccggaucaa caaucgguaa ggccuucgag gcuacuguca ggggcgcgaa acggauggca     1320

gugcuggggg acaccgccug ggauuuugga agu                                  1353


<210>  1895
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE CDS opt

<400>  1895
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga auuacaauau gccgguaccg acgggccuug uaaagugcca     1080

gcucagaugg ccguagauau gcagacgcug accccgguug gccgacucau cacagcaaau     1140

cccgugauaa cugaguccac cgaaaacagc aagaugaugc uugagcugga cccuccauuu     1200

ggagauucgu acauugucau cggcgugggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggccuucgag gcuaccguca ggggcgcgaa acggauggca     1320

guguuggggg acacagccug ggauuuugga uca                                  1353


<210>  1896
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE CDS opt

<400>  1896
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggca ucucuaacag ggacuucguu gaggggaugu caggagguac cuggguggau      120

guagugcugg aacacggcgg gugugucaca gugauggcac aagacaaacc uacuguugau      180

auugaguugg ugacgaccac agucaguaau auggccgaag ugcggagcua cugcuaugag      240

gcguccaucu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguaugc aaacgaacac ugguugaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauacaaccg gagaaucugg aguacagaau caugcuuagc      480

guccauggau cccagcacuc agguaugauu gugaacgaua caggccauga aaccgacgag      540

aauagggcua agguggaaau cacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcauuu ugggucucga uugcgagcca cgcaccgggc uggacuucag cgaucuguau      660

uaccucacaa ugaauaacaa acacugguua guccauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guugaguuca aggaugcaca cgccaagaga cagacagugg uaguguuggg cucucaggaa      840

ggggcugucc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucuccu cagggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugagcuaca gucucugcac ggccgcauuu acuuucacaa agauacccgc ugagacccug     1020

cacggcacug uuacagugga aguccaauau gccgguaccg acgggccuug uaaagugcca     1080

gcucagaugg ccguagauau gcagacguua accccgguug gccgacugau uacagcaaau     1140

cccgugauca cugagucuac cgaaaacucc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauagcu acauugucau cggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

ucgggaucua cuauugguaa ggccuucgag gcuaccguca ggggcgcgaa acggauggca     1320

gugcuggggg acacagccug ggauuuugga ucc                                  1353


<210>  1897
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE CDS opt

<400>  1897
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg uguuaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugaguuacu cucucugcac ggcggccuuu acuuucacaa agauucccgc agagacccug     1020

cacggcacug ucacagugga aguacaauau gcugguaccg acgggccuug uaaaguucca     1080

gcccagaugg cuguggauau gcagacguua accccggucg gccgacugau cacagccaau     1140

cccgugauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugucau cggcgugggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucuggaucca cuaucgguaa ggcauucgag gccaccguua ggggcgcuaa acggauggcg     1320

gugcuggggg acacagcaug ggauuuugga uca                                  1353


<210>  1898
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE CDS opt

<400>  1898
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccggugcug acgggccuug uaaaguacca     1080

gcccagaugg caguugauau gcagaccuua acgccggugg gccgacugau caccgccaau     1140

cccgucauaa cagaguccac ugaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

ucuggaucca caaucgguaa ggcuuucgag gcgacuguga ggggcgcaaa acggauggcc     1320

guucuggggg acaccgcuug ggauuuugga uca                                  1353


<210>  1899
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE CDS opt

<400>  1899
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acacuggagc ugacaccggc acaccacauu ggaauaacaa agaggcccuu      780

guggaguuca aggaugcgca cgcaaagaga cagaccguag uggucuuggg cucccaggaa      840

ggggccgugc auacggcucu ggccggagca cuagaagcug agauggacgg ugccaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ugcggccuuu acauucacca agauucccgc agagacucug     1020

cacggcacag ugaccgucga agugcaauau gcugguacgg acgggccuug uaaaguacca     1080

gcccagaugg cuguugauau gcagaccuua acaccggugg gccgacugau cacugccaau     1140

cccgucauaa ccgagggaac agaaaacucc aagaugaugc ucgagcuuga cccuccauuu     1200

ggcgauagcu acauugugau cggggucgga gaaaagaaaa uuacucauca cuggcauaga     1260

ucggguucua ccaucggcaa ggcauucgag gccacaguga ggggagcuaa acggauggcg     1320

guucuggggg acaccgcaug ggauuuuggc ucc                                  1353


<210>  1900
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE CDS opt

<400>  1900
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca gcaauggccc aagacaaacc uacuguugau      180

aucgaguugg ugacgaccac agucaguaau auggcggaag ugcggagcua cugcuaugag      240

gcuuccauuu cugacauggc cucggauuca cgcuguccaa cucagggaga agcauacuua      300

gacaagcaga gcgauaccca guauguaugc aaacgaacac ugguugaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgccaaauu cgcuuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugcug gugaacgaua caggccauga aaccgacgag      540

aauagggcaa agguggaaau cacgcccaac aguccucggg ccgaggcuac ucucggggga      600

uuuggcagcu ugggucugga uugcgagcca cgcaccgggc uggacuucuc ugaucucuau      660

uacuuaacaa ugaauaacaa acacuggcug gcccauaagg aaugguuuca cgacauuccc      720

cuuccuuggc acgcgggagc agccacuggc accccacauu ggaauaacaa agaggcuuug      780

gucgaguuca aggacgccca cgcaaagaga cagacaguug ugguacuggg cucccaggaa      840

ggggcugugc auaccgcccu agcgggagcc cucgaagcag agauggaugg ugcuaaaggc      900

aggcugucaa gcgggcaccu uaagugucgg cugaaaaugg acaaguugcg ccucaaagga      960

gucaguuacu cucugugcac ggccgcuuuu acuuucacaa agauccccgc cgagaccgug     1020

gauggcacug uuacagugga aggucaauau gggggcaccg acggaccuug uaaaguccca     1080

gcacagaugg ccguggauau gcagacguua accccgguag gccgacugau uacagcuaau     1140

cccguuauca cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggggauucgu acauagugau uggagucggu gaaaagaaaa ucacacacca uuggcacaga     1260

ucuggcucca cuauugggaa ggcguucgag gcaaccguga ggggagccaa acggauggcu     1320

guccugggcg acacagccug ggauuuuggu uca                                  1353


<210>  1901
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE CDS opt

<400>  1901
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc ugccguggau      180

aucgaguugg ucacuacgac cgugaguaau auggcggaag uacggagcua cugcuaugag      240

gcuuccauuu cugacauggc cucggauuca cgcuguccaa cacagggaga agcauacuua      300

gacaagcaga gcgauacuca guauguuugc aaacgaaccc ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgucacau gcgccaaauu cgcuuguucc      420

aagaagauga ccgggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

gugcauggau cccagcacuc agguaugauc gugaacgaua cuggccauga aacagacgag      540

aauagggcaa aggucgaaau uacccccaac aguccucggg ccgaggcuac gcugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcacugggc uggacuucuc ugaucuguau      660

uaccucacca ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgccggagc ggacacaggc acuccacauu ggaauaacaa agaggcacuu      780

guggaguuca aggaugccca cgcuaagaga cagaccguag uggucuuggg cucccaggaa      840

ggggccgugc auacagcacu ggcuggagcc cuagaagcgg agauggacgg ugccaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac cgcagcuuuu acguucacua agauucccgc cgagacacug     1020

cacggcaccg ugacugucga agugcaauau gcugguacag acgggccuug uaaaguacca     1080

gcccagaugg caguugauau gcagaccuua acgccggugg gccgacugau caccgccaau     1140

cccgucauaa cagaguccac ugaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

ucuggaucca caaucgguaa ggcuuucgag gcgacuguga ggggcgcaaa acggauggcc     1320

guucuggggg acaccgcuug ggauuuugga uca                                  1353


<210>  1902
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE CDS opt

<400>  1902
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuaacaauag ggacuucgug gaggggaugu cuggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugucaaac auggccgaag uacggaguua cugcuaugag      240

gcgagcauuu ccgacauggc uucugauucg cgcuguccaa cucagggaga agccuacuua      300

gacaagcagu cagauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aaugggugug gacuauuugg caagggcagc cucgugaccu gcgcaaaauu cgccuguagu      420

aagaagauga cugggaaauc cauccaaccg gagaaccugg aguacagaau aaugcuuucu      480

guccauggaa gccagcacuc cgguaugauc gugaaugaua caggccauga aaccgacgag      540

aacagggcua aggucgaaau uacgcccaau ucaccucggg cagaggccac ucugggaggg      600

uuuggcaguu ugggucucga uugcgagcca cgcaccgggc uggacuucag cgaucuguau      660

uaccucacaa ugaacaauaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaacaauaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucucaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucuccu cagggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuagcuaca gucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaac     1140

cccgucauaa cugagucuac cgaaaauucc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauagcu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

ucgggaucua cuaucgguaa ggccuucgag gcuaccguga ggggcgcgaa acggauggca     1320

guucuggggg acacagccug ggauuuugga ucc                                  1353


<210>  1903
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE CDS opt

<400>  1903
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcgc cgcagcuuuu acguucacua agauucccgc cgagacacug     1020

cacggcaccg ugacugucga agugcaauau gcugguacag acgggccuug uaaaguacca     1080

gcccagaugg caguugauau gcagaccuua acgccggugg gccgacugau caccgccaau     1140

cccgucauaa cagaguccac ugaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

ucuggaucca caaucgguaa ggcuuucgag gcgacuguga ggggcgcaaa acggauggcc     1320

guucuggggg acaccgcuug ggauuuugga uca                                  1353


<210>  1904
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE CDS opt

<400>  1904
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua uuggccauga aacagacgag      540

aauagggcua aggucgaaau cacccccaac aguccucggg cagaggccac gcugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcacugggc uggacuucuc ugaucuguau      660

uaccucacca ugaauaacaa acacugguua guucauaagg aaugguuuca cgacauuccc      720

cugccuuggc acgcuggagc cgacacaggc acuccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagaccguag uggucuuggg cucccaggaa      840

ggggcugugc auacagcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac cgccgcauuu acguucacua agauccccgc ugagacacug     1020

cacggcaccg ugacugucga agugcaauau gccgguacag acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagaccuua acgccggugg gccgacugau aaccgcaaau     1140

cccgucauua cagaguccac ugaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acaucgugau uggcgucggg gacaagaaaa ucacccauca cuggcauaga     1260

ucuggaucca caaucgguaa ggccuucgaa gcuacuguga ggggcgcgaa acggauggca     1320

guucuggggg auaccgccug ggacuuugga uca                                  1353


<210>  1905
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE CDS opt

<400>  1905
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cgccuguucc      420

aagaagauga cugggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua caggccauga aaccgacgag      540

aauagggcua aggucgaaau uacgcccaac aguccucggg cagaggccac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagaga cagacaguag uggucuuggg cucccaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

aggcucucaa gcgggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

guuaguuacu cucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gccgacugau cacagcaaau     1140

cccgucauaa cugaguccac cgaaaacagc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauucgu acauugugau cggcgucggg gacaagaaaa uuacacauca cugguauaga     1260

ucuggaucca cuaucgguaa ggccuucgaa gcuaccguga ggggcgcgaa acggauggca     1320

guucuggggg auacagccug ggacuuugga uca                                  1353


<210>  1906
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE CDS opt

<400>  1906
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua ccggccauga aacggacgag      540

aauagggcca aggucgaagu uacucccaac aguccucggg cugaggcaac ccugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcacagggc uggacuucuc ugaucuguau      660

uaccucacua ugaauaacaa acacugguua gugcauaagg aaugguuuca cgacauuccc      720

cugccuuggc acgccggagc ugacaccggc acaccacauu ggaauaacaa agaggcccuu      780

guagaguuca aggaugcgca cgcaaagaga cagaccgugg ucguguuggg cucccaggaa      840

ggggccguuc auacggcucu ggccggagca cuagaagcug agauggacgg ugccaaaggc      900

aggcucuuuu cagggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugagcuaca gucucugcac ugcggccuuc acauuuacca agguccccgc agagacucug     1020

cacggcacag ugaccguaga aguucaauau gcugguacgg acgggccuug uaaagugcca     1080

gcccagaugg cugucgauau gcagaccuua acaccggugg gccgacugau cacugccaau     1140

cccgucauua ccgagucuac agaaaacucc aagaugaugc ucgagcuuga cccuccauuc     1200

ggagauagcu acaucgugau aggcguuggg gacaagaaaa uuacucauca cuggcauaga     1260

ucgggaucua ccaucgguaa ggcauuugaa gccacaguga ggggcgcuaa acggauggcg     1320

guacuggggg auaccgcaug ggacuucgga ucc                                  1353


<210>  1907
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE CDS opt

<400>  1907
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua uuggccauga aaccgacgag      540

aauagggcca aggucgaagu uacgcccaac aguccucggg cugaggcaac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua gugcauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgccggagc ugacacuggc accccacauu ggaauaacaa agaggcccuu      780

guagaguuca aggaugcgca cgcaaagaga cagacagugg ucguguuggg cucccaggaa      840

ggggccguuc auaccgcucu ggccggagca cuagaagcug agauggacgg ugccaaaggc      900

aggcucuuuu cagggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugagcuaca gucucugcac ggcggccuuc acuuuuacaa agguccccgc agagacccug     1020

cacggcacug ugacaguaga aguucaauau gcugguaccg acgggccuug uaaagugcca     1080

gcccagaugg cugucgauau gcagacguua accccggugg gccgacugau uacagccaau     1140

cccgucauca cugagucuac cgaaaacucc aagaugaugc ucgagcuuga cccuccauuc     1200

ggagauagcu acauagugau uggcguuggg gacaagaaaa ucacacauca cuggcauaga     1260

ucgggaucua cuauugguaa ggcauuugaa gccaccguga ggggcgcuaa acggauggcg     1320

guacuggggg auacagcaug ggacuucgga ucc                                  1353


<210>  1908
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE CDS opt

<400>  1908
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua uuggccauga aaccgacgag      540

aauagggcca aggucgaagu uacgcccaac aguccucggg cugaggcaac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca cgcaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa acacugguua gugcauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgccggagc ugacacuggc accccacauu ggaauaacaa agaggcccuu      780

guagaguuca aggaugcgca cgcaaagaga cagacagugg ucguguuggg cucccaggaa      840

ggggccguuc auaccgcucu ggccggagca cuagaagcug agauggacgg ugccaaaggc      900

aggcucuuuu cagggcaccu gaagugucgg cuuaaaaugg auaagcugcg cuugaaagga      960

gugagcuaca gucucugcac ggcggucugu acugccgcaa aggugcccgc ugagacacug     1020

cacggcaccg uaacuguuga agugcaauau gccgguacag acgggccuug caaaguccca     1080

gcucagaugg ccguggauau gcagaccuua acgccggucg gccgacugau uaccgcaaau     1140

cccgugauca cagagucuac ugaaaacucc aagaugaugc ucgagcuuga cccuccauuc     1200

ggagauagcu acauaguuau uggcgugggg gacaagaaaa ucacccauca cuggcauaga     1260

ucgggaucua caauugguaa ggccuuugaa gcuacuguaa ggggcgcgaa acggauggca     1320

guccuggggg auaccgccug ggacuucgga ucc                                  1353


<210>  1909
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE CDS opt

<400>  1909
agccagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucaga       60

ugcauuggcg uuucuaacag ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacggagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgcuguccaa cucagggaga agccuacuua      300

gacaagcaga gcgauaccca guauguuugc aaacgaacac ugguggaccg uggcuggggu      360

aacgggugug gacuauuugg caagggcagu cucgugaccu gcgcaaaauu cacuuguucc      420

aagaagauga cagggaaauc uauccaaccg gagaaucugg aguacagaau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaug aaaauagggc caaggucgag      540

guuaccccca acaguccucg ggcugaagca acgcugggcg gguuuggcag cuugggacuc      600

gacugcgagc cacgcacugg ucuggauuuc ucugaccugu auuaccucac caugaauaac      660

aaacauuggu uagugcacaa ggagugguuu caugauauuc cccugccuug gcacuccggg      720

gccgacacag aaacuccaca cuggaauaac aaagaggcuc uuguagaguu caaggacgcc      780

caugcgaaga gacagaccgu ggucguguug ggaucacagg aaggcgcagu ucacacagcc      840

cuggcuggcg cccuagaagc agagauggau ggggcuaaag gaaggcucag caguggucau      900

cugaaguguc ggcuuaaaau ggacaagcug cgcuugaaag gcgugucuua cucccucugc      960

accgccgcgu uuacguucac uaaggucccc gccgagacac ugcacgggac cgugacugua     1020

gaaguucaau augcaggacg agauggcccu uguaaagugc cagcucagau ggccgucgac     1080

augcagacau uaaccccggu ggguagacug aucacggcua aucccgucau uaccgagagc     1140

acagaaaacu cgaagaugau gcucgagcuu gauccuccau uuggggacuc uuacaucgug     1200

auaggcguug gagauaagaa aauuacucac cauuggcaca gguccggcuc aaucauuggg     1260

aaggccuucg aagcaaccgu gcggggagcc aaacguaugg cuguacuggg ugacacagcg     1320

ugggauuuug gcagu                                                      1335


<210>  1910
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE CDS opt

<400>  1910
agccagaagg ugaucuaccu ggucaugauu guucucauag ugcccgccua uuccaucucu       60

ugcauuggcg uaucaaacag agaccuugug gaggggauga guggagguac cugggucgau      120

gugguucugg aacacggcgg gugugugaca gagauggcuc aagacaaacc uacugucgau      180

aucgaauugg ugacgaugac cguaagcaau auggcagagg uuagguccua cugcuaugaa      240

gccucuuuau cggacauggc gucagcuagc cgguguccaa cacagggaga gccgagucug      300

gauaagcagu ccgacacuca gucugugugc aaacgcaccc uaggcgaucg agguuggggg      360

aacggaugug gcauuuucgg caaggggagc cucgucacau gcuccaaauu uaccuguugc      420

aagaagaugc ccggaaaauc aauccaaccu gagaaucugg aauaccguau aaugcuucca      480

gugcauggua gucagcacag cggcaugauc gugaacgaca uugggcauga gacugaugaa      540

aauagagcca aggucgaggu uacacccaac ucuccuaggg cagaggccac ccugggaggc      600

uucgguuccu uggggcucga cugugaacca cggacgggac uggauuuuuc agaccuguau      660

uaccucacua ugaauaacaa acacugguua gugcauaagg agugguucca cgauaucccc      720

cugccuuggc acgcuggcgc agacaccggc acaccacauu ggaauaacaa agaagcccuu      780

guagaguuua aggacgcuca cgccaagcgc cagacugugg ucguguuggg gagccaggaa      840

ggagcgguuc auaccgcacu ggccggugcu cuagaggccg agauggaugg cgcaaaaggg      900

agacucuuca guggacaccu gaagugcagg cuuaaaaugg acaagcugcg guugaaaggc      960

gugucuuauu cccucuguac agcugccuuu accuucacga aggucccggc ggaaacucug     1020

cacgguacag ugaccguaga gguucaaagc gccgggacug auggccccug caaagugccu     1080

gcacagaugg cugucgacau gcagacauua accccagugg gacgccugau uacggccaau     1140

cccgucauca ccgaaucgac agagaacucu aagaugaugc ucgaacuuga uccuccauuu     1200

ggcgacuccu acauagugau ugggguugga gauaagaaaa ucacucauca cuggcaucga     1260

ucagguagua ccauuggcaa ggcuuucgag gccacaguga gaggggcaaa aaggauggcc     1320

guacugggag acacugcuug ggauuuuggc agc                                  1353


<210>  1911
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE CDS opt

<400>  1911
agacagaagg ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uagcaucagg       60

ugcauuggcg uuuccaaccg ggacuucgug gaggggaugu cuggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaaacc uacuguggau      180

aucgaguugg ucacgaccac agugucaaau auggccgaag uacgcaguua cugcuaugag      240

gcgagcauuu ccgacauggc uucugauucg cgauguccaa cucagggaga agccuacuua      300

gacaagcagu cagauaccca guauguuugc aaacguacac ugguggacag aggcuggggu      360

aacgggugug gacuauuugg caagggcagc cucgugaccu gcgcaaaauu cgccuguagu      420

aagaagauga cugggaaauc cauccaaccg gagaaucugg aguacaggau aaugcuuucu      480

guccauggaa gccagcacuc cgguaugauc gugaacgaua caggccauga aaccgacgag      540

aaucgggcua aggucgaaau uacgcccaac ucaccucgcg cagaggccac ucugggaggg      600

uuuggcaguu ugggucucga uugcgagcca agaaccgggc uggacuucag cgaucuguau      660

uaccucacaa ugaauaacaa acacugguua guucauaagg aaugguuuca cgacaucccc      720

cugccuuggc acgcuggagc cgacacuggc accccacauu ggaauaacaa agaggcgcuu      780

guggaguuca aggaugcaca cgccaagagg cagacaguag uggucuuggg cucucaggaa      840

ggggcugugc auaccgcccu ggcaggagcu cuagaagccg agauggacgg ugcgaaaggc      900

cggcucuccu cagggcaccu gaagugucgc cuuaaaaugg auaagcugcg auugaaagga      960

guuagcuaca gucucugcac ggccgcauuu acuuucacaa agauucccgc ugagacccug     1020

cacggcacug ugacagucga agugcaauau gccgguaccg acgggccuug uaaaguacca     1080

gcucagaugg ccguugauau gcagacguua accccggugg gcagacugau cacagcaaau     1140

cccgucauaa cugagucuac cgaaaacucc aagaugaugc ucgagcuuga cccuccauuu     1200

ggagauagcu acauugugau cggcgucggg gaaaagaaaa uuacacauca cuggcauagg     1260

ucgggaucua cuaucgguaa ggccuucgag gcuaccgugc ggggcgcgaa acguauggca     1320

guucuggggg acacagccug ggauuuugga ucc                                  1353


<210>  1912
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE CDS opt

<400>  1912
agccagagag ugaucuaccu ggucaugauu cuccuuauag cccccgcuua uuccaucagg       60

ugcauuggcg uuucuaaccg ggacuucgug gaggggaugu caggagguac cuggguagau      120

gugguccugg aacacggcgg gugugugaca guuauggcac aagacaagcc uacuguggau      180

aucgaguugg ucacgaccac agugaguaau auggccgaag uacgcagcua cugcuaugag      240

gcguccauuu cugacauggc uucggauuca cgauguccaa cucagggaga agccuacuua      300

gacaaacaga gcgauaccca guauguuugc aagcguacac ugguggacag aggcuggggu      360

aacgggugug gacuauuugg caaaggcagu cucgugaccu gcgcaaaguu cacuuguucc      420

aaaaagauga cagggaaguc uauccaaccg gagaaucugg aguacaggau aaugcuuagc      480

guccauggau cccagcacuc agguaugauc gugaacgaua uuggccauga aaccgacgag      540

aaucgggcca aagucgaagu uacgcccaac aguccucgcg cugaggcaac ucugggaggg      600

uuuggcagcu ugggucucga uugcgagcca agaaccgggc uggacuucuc ugaucuguau      660

uaccucacaa ugaauaacaa gcacugguua gugcauaaag aaugguuuca cgacaucccc      720

cugccuuggc acgccggagc ugacacuggc accccacauu ggaauaacaa ggaggcccuu      780

guagaguuca aagaugcgca cgcaaagagg cagacagugg ucguguuggg cucccaggaa      840

ggggccguuc auaccgcucu ggccggagca cuagaagcug agauggacgg ugccaagggc      900

cggcucuuuu cagggcaccu gaaaugucgc auuaagaugg auaaacuucg acugaaggga      960

gugagcuaca guuugugcac ggcggccuuc acuuuuucua aaguccccgc agagacacuc     1020

cacggcaccg ugacuguaga aguucaauau gcugguagag acgggccuug uaaggugcca     1080

gcccagaugg cugucgauau gcagacacug accccggugg gcagguuaau cacggccaau     1140

cccauaauua ccgaguccac agaaaacagc cggaugaugc uggagcucga cccuccauuc     1200

ggagauucgu acaucgucau uggcgugggg gacaaaaaga ucacucauca cuggcaucgu     1260

ucuggaucca ccaucgguaa ggcauuugaa gccacaguuc gcggcgcuaa aagaauggcg     1320

gugcuugggg auacugcaug ggacuucgga uca                                  1353


<210>  1913
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  1913
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  1914
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS opt

<400>  1914
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ccugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacgc caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  1915
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS opt

<400>  1915
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga cagagccaag     1080

guggagguga cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug     2040

accugccugg cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcagcg uggacuucag caag                                            2124


<210>  1916
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) CDS opt

<400>  1916
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  1917
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  1917
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1918
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) CDS opt

<400>  1918
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaccugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacgccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1919
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) CDS opt

<400>  1919
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucggcua cgagaccgac     1020

gaggacagag ccaaggugga ggugaccccc aacagcccca gagccgaggc cacccugggc     1080

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1140

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1320

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1380

ggcagacugu ucagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1440

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaagauc     1560

cccgugcaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1620

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1680

uucggcgaca gcuacaucgu gaucggcgug ggcgacaaga agaucaccca ccacuggcac     1740

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1800

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcguguu caacagccug     1860

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1920

ugguucagcc agauccugau cggcacccug cugguguggc ugggccugaa caccaagaac     1980

ggcagcauca gccugaccug ccuggcccug ggcggcguga ugaucuuccu gagcaccgcc     2040

gugagcgcc                                                             2049


<210>  1920
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) CDS opt

<400>  1920
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1921
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS opt

<400>  1921
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  1922
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS opt

<400>  1922
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  1923
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS opt

<400>  1923
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga cagagccaag      540

guggagguga cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug      600

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug      660

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac      720

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag      780

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac      840

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc      900

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc      960

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1020

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1080

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1140

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1200

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1260

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1320

accgccuggg acuucggcag c                                               1341


<210>  1924
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) CDS opt

<400>  1924
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  1925
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) CDS opt

<400>  1925
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug agcggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1926
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) CDS opt

<400>  1926
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaccugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug agcggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacgccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1927
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) CDS opt

<400>  1927
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug agcggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucggcua cgagaccgac     1020

gaggacagag ccaaggugga ggugaccccc aacagcccca gagccgaggc cacccugggc     1080

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1140

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1320

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1380

ggcagacugu ucagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1440

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaagauc     1560

cccgugcaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1620

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1680

uucggcgaca gcuacaucgu gaucggcgug ggcgacaaga agaucaccca ccacuggcac     1740

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1800

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcguguu caacagccug     1860

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1920

ugguucagcc agauccugau cggcacccug cugguguggc ugggccugaa caccaagaac     1980

ggcagcauca gccugaccug ccuggcccug ggcggcguga ugaucuuccu gagcaccgcc     2040

gugagcgcc                                                             2049


<210>  1928
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) CDS opt

<400>  1928
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug agcggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1929
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) CDS opt

<400>  1929
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugca ggacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1930
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) CDS opt

<400>  1930
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaccugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugca ggacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacgccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1931
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) CDS opt

<400>  1931
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugca ggacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  1932
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1932
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcacccug ggcaaggccu ucagcaccac ccugaagggc     1800

gcccagagac uggccgcccu gggcgacacc gccugggacu ucggcagcau cggcggcgug     1860

uucaacagca ucggcagagc cgugcaccag guguucggcg gcgccuucag aacccuguuc     1920

ggcggcauga gcuggaucac ccagggccug augggcgccc ugcugcugug gaugggcgug     1980

aacgccagag acagaagcau cgcccuggcc uuccuggcca ccggcggcgu gcugguguuc     2040

cuggccacca acgugcacgc c                                               2061


<210>  1933
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1933
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaccugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcacccug ggcaaggccu ucagcaccac ccugaagggc     1800

gcccagagac uggccgcccu gggcgacacc gccugggacu ucggcagcau cggcggcgug     1860

uucaacagca ucggcagagc cgugcaccag guguucggcg gcgccuucag aacccuguuc     1920

ggcggcauga gcuggaucac ccagggccug augggcgccc ugcugcugug gaugggcgug     1980

aacgccagag acagaagcau cgcccuggcc uuccuggcca ccggcggcgu gcugguguuc     2040

cuggccacca acgugcacgc c                                               2061


<210>  1934
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       CDS opt

<400>  1934
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucggcua cgagaccgac     1020

gaggacagag ccaaggugga ggugaccccc aacagcccca gagccgaggc cacccugggc     1080

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1140

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1320

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1380

ggcagacugu ucagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1440

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaagauc     1560

cccgugcaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1620

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1680

uucggcgaca gcuacaucgu gaucggcgug ggcgacaaga agaucaccca ccacuggcac     1740

agaagcggca gcacccuggg caaggccuuc agcaccaccc ugaagggcgc ccagagacug     1800

gccgcccugg gcgacaccgc cugggacuuc ggcagcaucg gcggcguguu caacagcauc     1860

ggcagagccg ugcaccaggu guucggcggc gccuucagaa cccuguucgg cggcaugagc     1920

uggaucaccc agggccugau gggcgcccug cugcugugga ugggcgugaa cgccagagac     1980

agaagcaucg cccuggccuu ccuggccacc ggcggcgugc ugguguuccu ggccaccaac     2040

gugcacgcc                                                             2049


<210>  1935
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  1935
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcacccug ggcaaggccu ucagcaccac ccugaagggc     1800

gcccagagac uggccgcccu gggcgacacc gccugggacu ucggcagcau cggcggcgug     1860

uucaacagca ucggcagagc cgugcaccag guguucggcg gcgccuucag aacccuguuc     1920

ggcggcauga gcuggaucac ccagggccug augggcgccc ugcugcugug gaugggcgug     1980

aacgccagag acagaagcau cgcccuggcc uuccuggcca ccggcggcgu gcugguguuc     2040

cuggccacca acgugcacgc c                                               2061


<210>  1936
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  1936
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  1937
<211>  2133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1937
aucaucaacg ccagaaagga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgacgugg gcugcagcgu ggacuucagc aag                                  2133


<210>  1938
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1938
aucaacgcca gaaaggagaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gacgugggcu gcagcgugga cuucagcaag                                      2130


<210>  1939
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1939
aacgccagaa aggagaagaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugca gcguggacuu cagcaag                                         2127


<210>  1940
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1940
gccagaaagg agaagaagag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcagcg uggacuucag caag                                            2124


<210>  1941
<211>  2121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1941
agaaaggaga agaagagaag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcagcgugg acuucagcaa g                                               2121


<210>  1942
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1942
aaggagaaga agagaagagg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

agcguggacu ucagcaag                                                   2118


<210>  1943
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1943
gagaagaaga gaagaggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcagc     2100

guggacuuca gcaag                                                      2115


<210>  1944
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1944
aagaagagaa gaggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     2100

gacuucagca ag                                                         2112


<210>  1945
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1945
aagagaagag gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cagcguggac     2100

uucagcaag                                                             2109


<210>  1946
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1946
agaagaggcg ccgacaccag cgugggcauc gugggccugc ugcugaccac cgccauggcc       60

gccgagguga ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc      120

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      180

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      300

ugccaccaca agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac      360

agcaccagaa agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag      420

caccugauca gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc      480

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      540

cugcugaucg cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug      600

gagggcauga gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc      660

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      720

auggccgagg ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      780

agaugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      840

aagagaaccc ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc      900

cuggugaccu gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc      960

gagaaccugg aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc     1020

gugaacgaca ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac     1080

agccccagag ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc     1140

agaaccggcc uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga     1320

cagaccgugg uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc     1380

cuggaggccg agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga     1440

cugaagaugg acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac     1560

gccggcaccg acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug     1620

acccccgugg gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc     1680

aagaugaugc uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc     1740

gagaagaaga ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag     1800

gccaccguga gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc     1860

agcgugggcg gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug     1980

auguggcugg gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc     2040

ggcgugcuga ucuuccugag caccgccgug agcgccgacg ugggcugcag cguggacuuc     2100

agcaag                                                                2106


<210>  1947
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1947
agaggcgccg acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc       60

gaggugacca gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag      120

gccaucagcu uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug      180

ggccacaugu gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc      300

caccacaaga agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc      360

accagaaagc ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac      420

cugaucagag uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc      480

aucgccuggc ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug      540

cugaucgccc ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag      600

ggcaugagcg gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug      660

auggcccagg acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug      720

gccgagguga gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga      780

ugccccaccc agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag      840

agaacccugg uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug      900

gugaccugcg ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag      960

aaccuggagu acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug     1020

aacgacaccg gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc     2040

gugcugaucu uccugagcac cgccgugagc gccgacgugg gcugcagcgu ggacuucagc     2100

aag                                                                   2103


<210>  1948
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1948
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag       60

gugaccagaa gaggcagcgc cuacuacaug uaccuggaca gaaacgacgc cggcgaggcc      120

aucagcuucc ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc      360

agaaagcugc agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug      420

aucagagugg agaacuggau cuucagaaac cccggcuucg cccuggccgc cgccgccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc      600

augagcggcg gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugagaa gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga      840

acccuggugg acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguaca gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaccggcc acgagaccga cgagaacaga gccaaggugg agaucacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag     1740

aagaucaccc accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugagaggcg ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgccc ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug     2040

cugaucuucc ugagcaccgc cgugagcgcc gacgugggcu gcagcgugga cuucagcaag     2100


<210>  1949
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1949
gccgacacca gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug       60

accagaagag gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc      120

agcuucccca ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac      180

augugcgacg ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac      240

gacguggacu gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac      300

aagaagggcg aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga      360

aagcugcaga ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc      420

agaguggaga acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc      480

uggcugcugg gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc      540

gcccccgccu acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug      600

agcggcggca ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc      660

caggacaagc ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag      720

gugagaagcu acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc      780

acccagggcg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc      840

cugguggaca gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc      900

ugcgccaagu ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug      960

gaguacagaa ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac     1020

accggccacg agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aagauccccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcgcccuga acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug     2040

aucuuccuga gcaccgccgu gagcgccgac gugggcugca gcguggacuu cagcaag        2097


<210>  1950
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1950
gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1800

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1860

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1920

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1980

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2040

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caag           2094


<210>  1951
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1951
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc cgacgugggc ugcagcgugg acuucagcaa g              2091


<210>  1952
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1952
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccgc cgugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc cgacgugggc ugcagcgugg acuucagcaa g              2091


<210>  1953
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1953
agaggcgccg acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc       60

gccgugacca gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag      120

gccaucagcu uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug      180

ggccacaugu gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc      300

caccacaaga agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc      360

accagaaagc ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac      420

cugaucagag uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc      480

aucgccuggc ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug      540

cugaucgccc ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag      600

ggcaugagcg gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug      660

auggcccagg acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug      720

gccgagguga gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga      780

ugccccaccc agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag      840

agaacccugg uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug      900

gugaccugcg ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag      960

aaccuggagu acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug     1020

aacgacaccg gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc     2040

gugcugaucu uccugagcac cgccgugagc gccgacgugg gcugcagcgu ggacuucagc     2100

aag                                                                   2103


<210>  1954
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1954
agaggccuga gcagcagaaa gagaagaagc cacgacgugc ugaccgugca guuccugauc       60

cugggcaugc ugcugaugac cggcggcgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugca gcguggacuu cagcaag                                         2127


<210>  1955
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1955
agaggccuga gcagcagaaa gagaagaagc cacgacgugc ugaccgugca guuccugauc       60

cugggcaugc ugcugaugac cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugca gcguggacuu cagcaag                                         2127


<210>  1956
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1956
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugc                                                   2118


<210>  1957
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1957
aucaucaacg ccagaaagga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgacgugg gcugc                                                      2115


<210>  1958
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1958
aucaacgcca gaaaggagaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gacgugggcu gc                                                         2112


<210>  1959
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1959
aacgccagaa aggagaagaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugc                                                             2109


<210>  1960
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1960
gccagaaagg agaagaagag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugc                                                                2106


<210>  1961
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1961
agaaaggaga agaagagaag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugc                                                                   2103


<210>  1962
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1962
aaggagaaga agagaagagg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100


<210>  1963
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1963
gagaagaaga gaagaggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugc        2097


<210>  1964
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1964
aagaagagaa gaggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugc           2094


<210>  1965
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1965
aagagaagag gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug c              2091


<210>  1966
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1966
agaagaggcg ccgacaccag cgugggcauc gugggccugc ugcugaccac cgccauggcc       60

gccgagguga ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc      120

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      180

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      300

ugccaccaca agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac      360

agcaccagaa agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag      420

caccugauca gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc      480

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      540

cugcugaucg cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug      600

gagggcauga gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc      660

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      720

auggccgagg ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      780

agaugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      840

aagagaaccc ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc      900

cuggugaccu gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc      960

gagaaccugg aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc     1020

gugaacgaca ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac     1080

agccccagag ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc     1140

agaaccggcc uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga     1320

cagaccgugg uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc     1380

cuggaggccg agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga     1440

cugaagaugg acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac     1560

gccggcaccg acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug     1620

acccccgugg gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc     1680

aagaugaugc uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc     1740

gagaagaaga ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag     1800

gccaccguga gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc     1860

agcgugggcg gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug     1980

auguggcugg gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc     2040

ggcgugcuga ucuuccugag caccgccgug agcgccgacg ugggcugc                  2088


<210>  1967
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1967
agaggcgccg acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc       60

gaggugacca gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag      120

gccaucagcu uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug      180

ggccacaugu gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc      300

caccacaaga agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc      360

accagaaagc ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac      420

cugaucagag uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc      480

aucgccuggc ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug      540

cugaucgccc ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag      600

ggcaugagcg gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug      660

auggcccagg acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug      720

gccgagguga gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga      780

ugccccaccc agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag      840

agaacccugg uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug      900

gugaccugcg ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag      960

aaccuggagu acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug     1020

aacgacaccg gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc     2040

gugcugaucu uccugagcac cgccgugagc gccgacgugg gcugc                     2085


<210>  1968
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1968
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag       60

gugaccagaa gaggcagcgc cuacuacaug uaccuggaca gaaacgacgc cggcgaggcc      120

aucagcuucc ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc      360

agaaagcugc agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug      420

aucagagugg agaacuggau cuucagaaac cccggcuucg cccuggccgc cgccgccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc      600

augagcggcg gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugagaa gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga      840

acccuggugg acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguaca gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaccggcc acgagaccga cgagaacaga gccaaggugg agaucacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag     1740

aagaucaccc accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugagaggcg ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgccc ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug     2040

cugaucuucc ugagcaccgc cgugagcgcc gacgugggcu gc                        2082


<210>  1969
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1969
gccgacacca gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug       60

accagaagag gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc      120

agcuucccca ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac      180

augugcgacg ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac      240

gacguggacu gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac      300

aagaagggcg aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga      360

aagcugcaga ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc      420

agaguggaga acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc      480

uggcugcugg gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc      540

gcccccgccu acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug      600

agcggcggca ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc      660

caggacaagc ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag      720

gugagaagcu acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc      780

acccagggcg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc      840

cugguggaca gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc      900

ugcgccaagu ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug      960

gaguacagaa ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac     1020

accggccacg agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aagauccccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcgcccuga acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug     2040

aucuuccuga gcaccgccgu gagcgccgac gugggcugc                            2079


<210>  1970
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1970
gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1800

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1860

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1920

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1980

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2040

uuccugagca ccgccgugag cgccgacgug ggcugc                               2076


<210>  1971
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1971
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc cgacgugggc ugc                                  2073


<210>  1972
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1972
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccgc cgugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc cgacgugggc ugc                                  2073


<210>  1973
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1973
agaggcgccg acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc       60

gccgugacca gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag      120

gccaucagcu uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug      180

ggccacaugu gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc      300

caccacaaga agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc      360

accagaaagc ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac      420

cugaucagag uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc      480

aucgccuggc ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug      540

cugaucgccc ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag      600

ggcaugagcg gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug      660

auggcccagg acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug      720

gccgagguga gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga      780

ugccccaccc agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag      840

agaacccugg uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug      900

gugaccugcg ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag      960

aaccuggagu acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug     1020

aacgacaccg gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc     2040

gugcugaucu uccugagcac cgccgugagc gccgacgugg gcugc                     2085


<210>  1974
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1974
agaggccuga gcagcagaaa gagaagaagc cacgacgugc ugaccgugca guuccugauc       60

cugggcaugc ugcugaugac cggcggcgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugc                                                             2109


<210>  1975
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1975
agaggccuga gcagcagaaa gagaagaagc cacgacgugc ugaccgugca guuccugauc       60

cugggcaugc ugcugaugac cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugc                                                             2109


<210>  1976
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1976
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgac                                                             2109


<210>  1977
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1977
aucaucaacg ccagaaagga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgac                                                                2106


<210>  1978
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1978
aucaacgcca gaaaggagaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gac                                                                   2103


<210>  1979
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1979
aacgccagaa aggagaagaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100


<210>  1980
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1980
gccagaaagg agaagaagag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgac        2097


<210>  1981
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1981
agaaaggaga agaagagaag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgac           2094


<210>  1982
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1982
aaggagaaga agagaagagg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga c              2091


<210>  1983
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1983
gagaagaaga gaagaggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgac                  2088


<210>  1984
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1984
aagaagagaa gaggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgac                     2085


<210>  1985
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1985
aagagaagag gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg ac                        2082


<210>  1986
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1986
agaagaggcg ccgacaccag cgugggcauc gugggccugc ugcugaccac cgccauggcc       60

gccgagguga ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc      120

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      180

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      300

ugccaccaca agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac      360

agcaccagaa agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag      420

caccugauca gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc      480

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      540

cugcugaucg cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug      600

gagggcauga gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc      660

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      720

auggccgagg ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      780

agaugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      840

aagagaaccc ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc      900

cuggugaccu gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc      960

gagaaccugg aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc     1020

gugaacgaca ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac     1080

agccccagag ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc     1140

agaaccggcc uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga     1320

cagaccgugg uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc     1380

cuggaggccg agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga     1440

cugaagaugg acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac     1560

gccggcaccg acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug     1620

acccccgugg gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc     1680

aagaugaugc uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc     1740

gagaagaaga ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag     1800

gccaccguga gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc     1860

agcgugggcg gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug     1980

auguggcugg gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc     2040

ggcgugcuga ucuuccugag caccgccgug agcgccgac                            2079


<210>  1987
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1987
agaggcgccg acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc       60

gaggugacca gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag      120

gccaucagcu uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug      180

ggccacaugu gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc      300

caccacaaga agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc      360

accagaaagc ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac      420

cugaucagag uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc      480

aucgccuggc ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug      540

cugaucgccc ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag      600

ggcaugagcg gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug      660

auggcccagg acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug      720

gccgagguga gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga      780

ugccccaccc agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag      840

agaacccugg uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug      900

gugaccugcg ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag      960

aaccuggagu acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug     1020

aacgacaccg gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc     2040

gugcugaucu uccugagcac cgccgugagc gccgac                               2076


<210>  1988
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1988
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag       60

gugaccagaa gaggcagcgc cuacuacaug uaccuggaca gaaacgacgc cggcgaggcc      120

aucagcuucc ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc      360

agaaagcugc agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug      420

aucagagugg agaacuggau cuucagaaac cccggcuucg cccuggccgc cgccgccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc      600

augagcggcg gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugagaa gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga      840

acccuggugg acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguaca gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaccggcc acgagaccga cgagaacaga gccaaggugg agaucacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag     1740

aagaucaccc accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugagaggcg ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgccc ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug     2040

cugaucuucc ugagcaccgc cgugagcgcc gac                                  2073


<210>  1989
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1989
gccgacacca gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug       60

accagaagag gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc      120

agcuucccca ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac      180

augugcgacg ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac      240

gacguggacu gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac      300

aagaagggcg aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga      360

aagcugcaga ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc      420

agaguggaga acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc      480

uggcugcugg gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc      540

gcccccgccu acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug      600

agcggcggca ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc      660

caggacaagc ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag      720

gugagaagcu acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc      780

acccagggcg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc      840

cugguggaca gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc      900

ugcgccaagu ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug      960

gaguacagaa ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac     1020

accggccacg agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aagauccccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcgcccuga acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug     2040

aucuuccuga gcaccgccgu gagcgccgac                                      2070


<210>  1990
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1990
gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1800

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1860

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1920

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1980

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2040

uuccugagca ccgccgugag cgccgac                                         2067


<210>  1991
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1991
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc cgac                                            2064


<210>  1992
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1992
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccgc cgugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc cgac                                            2064


<210>  1993
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1993
agaggcgccg acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc       60

gccgugacca gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag      120

gccaucagcu uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug      180

ggccacaugu gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc      300

caccacaaga agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc      360

accagaaagc ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac      420

cugaucagag uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc      480

aucgccuggc ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug      540

cugaucgccc ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag      600

ggcaugagcg gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug      660

auggcccagg acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug      720

gccgagguga gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga      780

ugccccaccc agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag      840

agaacccugg uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug      900

gugaccugcg ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag      960

aaccuggagu acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug     1020

aacgacaccg gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc     2040

gugcugaucu uccugagcac cgccgugagc gccgac                               2076


<210>  1994
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1994
agaggccuga gcagcagaaa gagaagaagc cacgacgugc ugaccgugca guuccugauc       60

cugggcaugc ugcugaugac cggcggcgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100


<210>  1995
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1995
agaggccuga gcagcagaaa gagaagaagc cacgacgugc ugaccgugca guuccugauc       60

cugggcaugc ugcugaugac cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100


<210>  1996
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1996
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgcc                                                                2106


<210>  1997
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1997
aucaacgcca gaaaggagaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100


<210>  1998
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1998
aucaucaacg ccagaaagga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gcc                                                                   2103


<210>  1999
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  1999
aacgccagaa aggagaagaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgcc        2097


<210>  2000
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2000
gccagaaagg agaagaagag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgcc           2094


<210>  2001
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2001
agaaaggaga agaagagaag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc c              2091


<210>  2002
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2002
aaggagaaga agagaagagg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgcc                  2088


<210>  2003
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2003
gagaagaaga gaagaggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgcc                     2085


<210>  2004
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2004
aagaagagaa gaggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg cc                        2082


<210>  2005
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2005
aagagaagag gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgcc                            2079


<210>  2006
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2006
agaagaggcg ccgacaccag cgugggcauc gugggccugc ugcugaccac cgccauggcc       60

gccgagguga ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc      120

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      180

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      300

ugccaccaca agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac      360

agcaccagaa agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag      420

caccugauca gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc      480

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      540

cugcugaucg cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug      600

gagggcauga gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc      660

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      720

auggccgagg ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      780

agaugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      840

aagagaaccc ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc      900

cuggugaccu gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc      960

gagaaccugg aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc     1020

gugaacgaca ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac     1080

agccccagag ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc     1140

agaaccggcc uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga     1320

cagaccgugg uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc     1380

cuggaggccg agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga     1440

cugaagaugg acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac     1560

gccggcaccg acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug     1620

acccccgugg gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc     1680

aagaugaugc uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc     1740

gagaagaaga ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag     1800

gccaccguga gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc     1860

agcgugggcg gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug     1980

auguggcugg gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc     2040

ggcgugcuga ucuuccugag caccgccgug agcgcc                               2076


<210>  2007
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2007
agaggcgccg acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc       60

gaggugacca gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag      120

gccaucagcu uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug      180

ggccacaugu gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc      300

caccacaaga agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc      360

accagaaagc ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac      420

cugaucagag uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc      480

aucgccuggc ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug      540

cugaucgccc ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag      600

ggcaugagcg gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug      660

auggcccagg acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug      720

gccgagguga gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga      780

ugccccaccc agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag      840

agaacccugg uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug      900

gugaccugcg ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag      960

aaccuggagu acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug     1020

aacgacaccg gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc     2040

gugcugaucu uccugagcac cgccgugagc gcc                                  2073


<210>  2008
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2008
ggcgccgaca ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag       60

gugaccagaa gaggcagcgc cuacuacaug uaccuggaca gaaacgacgc cggcgaggcc      120

aucagcuucc ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc      360

agaaagcugc agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug      420

aucagagugg agaacuggau cuucagaaac cccggcuucg cccuggccgc cgccgccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc      600

augagcggcg gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugagaa gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga      840

acccuggugg acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguaca gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaccggcc acgagaccga cgagaacaga gccaaggugg agaucacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaagaucc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag     1740

aagaucaccc accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugagaggcg ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgccc ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug     2040

cugaucuucc ugagcaccgc cgugagcgcc                                      2070


<210>  2009
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2009
gccgacacca gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug       60

accagaagag gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc      120

agcuucccca ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac      180

augugcgacg ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac      240

gacguggacu gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac      300

aagaagggcg aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga      360

aagcugcaga ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc      420

agaguggaga acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc      480

uggcugcugg gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc      540

gcccccgccu acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug      600

agcggcggca ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc      660

caggacaagc ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag      720

gugagaagcu acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc      780

acccagggcg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc      840

cugguggaca gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc      900

ugcgccaagu ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug      960

gaguacagaa ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac     1020

accggccacg agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aagauccccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcgcccuga acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug     2040

aucuuccuga gcaccgccgu gagcgcc                                         2067


<210>  2010
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2010
gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1800

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1860

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1920

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1980

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2040

uuccugagca ccgccgugag cgcc                                            2064


<210>  2011
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  2011
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2012
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2012
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccgc cgugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2013
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2013
agaggcgccg acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc       60

gccgugacca gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag      120

gccaucagcu uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug      180

ggccacaugu gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag      240

cccgacgacg uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc      300

caccacaaga agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc      360

accagaaagc ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac      420

cugaucagag uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc      480

aucgccuggc ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug      540

cugaucgccc ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag      600

ggcaugagcg gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug      660

auggcccagg acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug      720

gccgagguga gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga      780

ugccccaccc agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag      840

agaacccugg uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug      900

gugaccugcg ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag      960

aaccuggagu acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug     1020

aacgacaccg gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaaga uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc     2040

gugcugaucu uccugagcac cgccgugagc gcc                                  2073


<210>  2014
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2014
agaggccuga gcagcagaaa gagaagaagc cacgacgugc ugaccgugca guuccugauc       60

cugggcaugc ugcugaugac cggcggcgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgcc        2097


<210>  2015
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2015
agaggccuga gcagcagaaa gagaagaagc cacgacgugc ugaccgugca guuccugauc       60

cugggcaugc ugcugaugac cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgcc        2097


<210>  2016
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long CDS opt

<400>  2016
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2017
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long CDS opt

<400>  2017
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ccugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacgc caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2018
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long CDS opt

<400>  2018
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga cagagccaag     1080

guggagguga cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug     2040

accugccugg cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcagcg uggacuucag caag                                            2124


<210>  2019
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long CDS opt

<400>  2019
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2020
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long CDS opt

<400>  2020
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggcuacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

aagcuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caagaucccc     1620

gugcagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2021
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long CDS opt

<400>  2021
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucacccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgacg agaacagagc caagguggag     1080

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcguguuc aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugguguggcu gggccugaac accaagaacg gcagcaucag ccugaccugc     2040

cuggcccugg gcggcgugau gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

agcguggacu ucagcaag                                                   2118


<210>  2022
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long CDS opt

<400>  2022
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccacagaa agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggcuacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

aagcuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caagaucccc     1620

gugcagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2023
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long CDS opt

<400>  2023
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugacc gugaacgaca ucggcuacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

aagcuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caagaucccc     1620

gugcagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2024
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long CDS opt

<400>  2024
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagaa cagagccaag     1080

guggagguga cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcaa gcuguucagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug     2040

accugccugg cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcagcg uggacuucag caag                                            2124


<210>  2025
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long CDS opt

<400>  2025
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

agaggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2026
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long CDS opt

<400>  2026
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacgccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2027
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long CDS opt

<400>  2027
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gccuggcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2028
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long CDS opt

<400>  2028
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cugguacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2029
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long CDS opt

<400>  2029
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgaga ucagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2030
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long CDS opt

<400>  2030
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugcug     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2031
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long CDS opt

<400>  2031
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccacccugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2032
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long CDS opt

<400>  2032
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccguggug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2033
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long CDS opt

<400>  2033
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagcg ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaccccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2034
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long CDS opt

<400>  2034
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agcaagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2035
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long CDS opt

<400>  2035
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agacagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2036
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long CDS opt

<400>  2036
agaaucauca acgccagaaa ggagaagaag agaagaagcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacac ccagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2037
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long CDS opt

<400>  2037
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccaa cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccag aagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2038
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long CDS opt

<400>  2038
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgagaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2039
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long CDS opt

<400>  2039
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagccugaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2040
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long CDS opt

<400>  2040
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccaa cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggauguuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2041
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long CDS opt

<400>  2041
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggauccug      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcaucggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2042
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long CDS opt

<400>  2042
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugagcaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccaccauca gcgacaucgc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgccg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2043
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long CDS opt

<400>  2043
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cacccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2044
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long CDS opt

<400>  2044
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga gcugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2045
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long CDS opt

<400>  2045
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgagaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaaucgagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2046
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long CDS opt

<400>  2046
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcau cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2047
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long CDS opt

<400>  2047
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgugaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcu ucaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2048
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long CDS opt

<400>  2048
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

aagcugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2049
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long CDS opt

<400>  2049
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggaggccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2050
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long CDS opt

<400>  2050
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcgccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2051
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long CDS opt

<400>  2051
agaaucauca acgccggcaa ggagaagaag agaagaggcg ccgacaccaa cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccag aagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2052
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long CDS opt

<400>  2052
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgagaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa ggacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2053
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long CDS opt

<400>  2053
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggu gagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2054
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long CDS opt

<400>  2054
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccaa cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

augaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2055
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long CDS opt

<400>  2055
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccgc ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga cccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2056
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long CDS opt

<400>  2056
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgagaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccuggccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2057
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long CDS opt

<400>  2057
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggca ucagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcaucc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2058
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long CDS opt

<400>  2058
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ccugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaucagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacgc caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2059
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long CDS opt

<400>  2059
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggcccagaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2060
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long CDS opt

<400>  2060
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcaggc cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2061
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long CDS opt

<400>  2061
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2062
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long CDS opt

<400>  2062
agaaucauca acgccagaaa ggagaagaag agaagaggca ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acaccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagggcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgugcugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2063
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long CDS opt

<400>  2063
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gccauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugcug gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gcccacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgccaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagaccgug     1560

gacggcaccg ugaccgugga gggccaguac ggcggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucau cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcggcggcg ugggcugcag cguggacuuc agcaag                               2136


<210>  2064
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long CDS opt

<400>  2064
agaaucauca acgccagaaa ggagaagaag agaagaggca ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucagca agauccccgc cgagacccug     1560

cacggcaccg ugaccgagga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2065
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long CDS opt

<400>  2065
agaaucauca acgccagaaa ggagaagaag agaagaggca ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2066
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long CDS opt

<400>  2066
agaaucauca acgccagaaa ggagaagaag agaagaggca ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc guggagguga ccagaagagg caacgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac caccaugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2067
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long CDS opt

<400>  2067
agaaucauca acgccagaaa ggagaagaag agaagaggca ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc uggugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2068
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long CDS opt

<400>  2068
agaaucauca acgccagaaa ggagaagaag agaagaggca ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc guggagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc cgccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2069
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long CDS opt

<400>  2069
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc guggagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gugaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2070
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long CDS opt

<400>  2070
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc guggagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc gaggccauca gcuuccccac cacccugggc      180

gugaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cugguacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2071
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long CDS opt

<400>  2071
agaaucauca acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc guggagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuuccccac cacccugggc      180

gugaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2072
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long CDS opt

<400>  2072
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucacccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2073
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long CDS opt

<400>  2073
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac caaccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcgugc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2074
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long CDS opt

<400>  2074
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2075
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long CDS opt

<400>  2075
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac caaccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgacg agaacagagc caagguggag     1080

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcguguuc aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugguguggcu gggccugaac accaagaacg gcagcaucag ccugaccugc     2040

cuggcccugg gcggcgugau gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

agcguggacu ucagcaag                                                   2118


<210>  2076
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long CDS opt

<400>  2076
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2077
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long CDS opt

<400>  2077
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccagcaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgugugc accgccgcca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2078
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long CDS opt

<400>  2078
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacgccag caucggcauc       60

gugagccugc ugcugaccac cgugauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgugac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgacg agaacagagc caagguggag     1080

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacagcggc     1260

gccgacaccg agacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcag agacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caucaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcguguuc aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugguguggcu gggccugaac accaagaacg gcagcaucag ccugaccugc     2040

cuggcccugg gcggcgugau gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

agcguggacu ucagcaag                                                   2118


<210>  2079
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long CDS opt

<400>  2079
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgacg agaacagagc caagguggag     1080

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcguguuc aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugguguggcu gggccugaac accaagaacg gcagcaucag ccugaccugc     2040

cuggcccugg gcggcgugau gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

agcguggacu ucagcaag                                                   2118


<210>  2080
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long CDS opt

<400>  2080
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac cacccugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga gaccagaaga      360

agcagaagaa gcgugagccu gagauaccac uacaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaagaag caccugauca ugguggagaa cuggaucuuc      480

agaaaccccg gcuucgccau cgugagcgug gccaucaccu ggcugauggg cagccugacc      540

agccagaagg ugaucuaccu ggugaugauc gugcugaucg ugcccgccua cagcaucagc      600

ugcaucggcg ugagcaacag agaccuggug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gagauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaugac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagccuga gcgacauggc cagcgccagc agaugcccca cccagggcga gcccagccug      840

gacaagcaga gcgacaccca gagcgugugc aagagaaccc ugggcgacag aggcuggggc      900

aacggcugcg gcaucuucgg caagggcagc cuggugaccu gcagcaaguu caccugcugc      960

aagaagaugc ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugccc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcagagc gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaag                               2136


<210>  2081
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long CDS opt

<400>  2081
agaaucauca acgccagaaa ggagagaaag agaagaggcg ccgacaccag caucggcauc       60

aucggccugc ugcugaccac cgccauggcc gccgagauca ccagaagagg cagcgccuac      120

uacauguacc uggacagaag cgacgccggc aaggccauca gcuucgccac caccaugggc      180

gugaacaagu gccacgugca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaccc      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggauccug      480

agaaaccccg gcuucaccgu ggccgcccug gccauggccu ggcugcuggg caacagcacc      540

agccagagag ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga aucaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucagca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcagag acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccaucauca ccgagagcac cgagaacagc agaaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcgugc accagguguu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc uggccugccu ggcccugggc ggcgccauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc                                      2130


<210>  2082
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short CDS opt

<400>  2082
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2083
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short CDS opt

<400>  2083
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaccugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacgccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2084
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short CDS opt

<400>  2084
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucggcua cgagaccgac     1020

gaggacagag ccaaggugga ggugaccccc aacagcccca gagccgaggc cacccugggc     1080

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1140

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1320

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1380

ggcagacugu ucagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1440

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaagauc     1560

cccgugcaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1620

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1680

uucggcgaca gcuacaucgu gaucggcgug ggcgacaaga agaucaccca ccacuggcac     1740

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1800

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcguguu caacagccug     1860

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1920

ugguucagcc agauccugau cggcacccug cugguguggc ugggccugaa caccaagaac     1980

ggcagcauca gccugaccug ccuggcccug ggcggcguga ugaucuuccu gagcaccgcc     2040

gugagcgcc                                                             2049


<210>  2085
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short CDS opt

<400>  2085
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2086
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short CDS opt

<400>  2086
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

uacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcaagcu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaaga uccccgugca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2087
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short CDS opt

<400>  2087
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca cagaaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

uacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcaagcu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaaga uccccgugca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2088
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short CDS opt

<400>  2088
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaccgugaa cgacaucggc     1020

uacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcaagcu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaaga uccccgugca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2089
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short CDS opt

<400>  2089
accagcaucg gcaucguggg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc acccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacgagaac     1020

agagccaagg uggaggugac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1080

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1140

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1200

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1260

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1320

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1380

cuguucagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1440

agcuacagcc ugugcaccgc cgccuucacc uucaccaagg ugcccgccga gacccugcac     1500

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1560

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1620

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1680

gacagcuaca ucgugaucgg cgugggcgac aagaagauca cccaccacug gcacagaagc     1740

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1800

cugggcgaca ccgccuggga cuucggcagc gugggcggcg uguucaacag ccugggcaag     1860

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1920

agccagaucc ugaucggcac ccugcuggug uggcugggcc ugaacaccaa gaacggcagc     1980

aucagccuga ccugccuggc ccugggcggc gugaugaucu uccugagcac cgccgugagc     2040

gcc                                                                   2043


<210>  2090
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short CDS opt

<400>  2090
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucggcua cgagaccgac     1020

gagaacagag ccaaggugga ggugaccccc aacagcccca gagccgaggc cacccugggc     1080

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1140

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1200

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1260

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1320

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1380

ggcaagcugu ucagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1440

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc     1500

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaagauc     1560

cccgugcaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1620

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1680

uucggcgaca gcuacaucgu gaucggcgug ggcgacaaga agaucaccca ccacuggcac     1740

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1800

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcguguu caacagccug     1860

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1920

ugguucagcc agauccugau cggcacccug cugguguggc ugggccugaa caccaagaac     1980

ggcagcauca gccugaccug ccuggcccug ggcggcguga ugaucuuccu gagcaccgcc     2040

gugagcgcc                                                             2049


<210>  2091
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short CDS opt

<400>  2091
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcagagg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2092
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short CDS opt

<400>  2092
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggccug gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2093
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short CDS opt

<400>  2093
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggu acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2094
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short CDS opt

<400>  2094
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacacccaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2095
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short CDS opt

<400>  2095
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgagaucaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2096
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short CDS opt

<400>  2096
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcuggccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2097
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short CDS opt

<400>  2097
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccacc cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2098
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short CDS opt

<400>  2098
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg uggugagcgc c                                               2061


<210>  2099
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short CDS opt

<400>  2099
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcgccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagac cccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2100
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short CDS opt

<400>  2100
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcaa gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2101
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short CDS opt

<400>  2101
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagaca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2102
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short CDS opt

<400>  2102
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcaccc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2103
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short CDS opt

<400>  2103
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagcc ugagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2104
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short CDS opt

<400>  2104
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagaauc      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2105
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short CDS opt

<400>  2105
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga uccugagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggca ucggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2106
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short CDS opt

<400>  2106
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugagc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccac caucagcgac aucgccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgccguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2107
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short CDS opt

<400>  2107
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggagcugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2108
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short CDS opt

<400>  2108
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaucgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2109
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short CDS opt

<400>  2109
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcguga agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagcuucaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2110
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short CDS opt

<400>  2110
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcaagcu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2111
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short CDS opt

<400>  2111
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaaggacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2112
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short CDS opt

<400>  2112
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggag gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2113
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short CDS opt

<400>  2113
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccgcccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagacccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2114
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short CDS opt

<400>  2114
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg cgccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2115
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short CDS opt

<400>  2115
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgcccug      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2116
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short CDS opt

<400>  2116
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggugagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2117
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short CDS opt

<400>  2117
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcaucagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cauccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2118
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short CDS opt

<400>  2118
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaccugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcau cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacgccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2119
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short CDS opt

<400>  2119
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggccc agagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2120
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short CDS opt

<400>  2120
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

caggccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2121
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short CDS opt

<400>  2121
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu cagcaagauc     1500

cccgccgaga cccugcacgg caccgugacc gaggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2122
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short CDS opt

<400>  2122
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacacc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag ggcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2123
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short CDS opt

<400>  2123
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2124
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short CDS opt

<400>  2124
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgugga ggugaccaga       60

agaggcaacg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccacca ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2125
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short CDS opt

<400>  2125
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccuggug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2126
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short CDS opt

<400>  2126
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugaac aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccuggug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2127
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short CDS opt

<400>  2127
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgugga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccgccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2128
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short CDS opt

<400>  2128
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgugga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgugau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2129
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short CDS opt

<400>  2129
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgugga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcgugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggu acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2130
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short CDS opt

<400>  2130
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgugga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

cccaccaccc ugggcgugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaucggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2131
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short CDS opt

<400>  2131
accagcaucg gcaucguggg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc acccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2132
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short CDS opt

<400>  2132
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaacc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaucggc     1020

cacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cgugcaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2133
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short CDS opt

<400>  2133
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaucggc     1020

cacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2134
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short CDS opt

<400>  2134
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaacc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacgagaac     1020

agagccaagg uggaggugac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1080

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1140

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1200

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1260

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1320

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1380

cuguucagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1440

agcuacagcc ugugcaccgc cgccuucacc uucaccaagg ugcccgccga gacccugcac     1500

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1560

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1620

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1680

gacagcuaca ucgugaucgg cgugggcgac aagaagauca cccaccacug gcacagaagc     1740

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1800

cugggcgaca ccgccuggga cuucggcagc gugggcggcg uguucaacag ccugggcaag     1860

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1920

agccagaucc ugaucggcac ccugcuggug uggcugggcc ugaacaccaa gaacggcagc     1980

aucagccuga ccugccuggc ccugggcggc gugaugaucu uccugagcac cgccgugagc     2040

gcc                                                                   2043


<210>  2135
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short CDS opt

<400>  2135
accagcaucg gcaucguggg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2136
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short CDS opt

<400>  2136
accagcaucg gcaucguggg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca gcacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaucggc     1020

cacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ugugcaccgc cgccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2137
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short CDS opt

<400>  2137
accagcaucg gcaucguggg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucaaggug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggugg ccguggccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucaccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacgagaac     1020

agagccaagg uggaggugac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1080

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1140

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1200

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1260

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1320

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1380

cuguucagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1440

agcuacagcc ugugcaccgc cgccuucacc uucaccaagg ugcccgccga gacccugcac     1500

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1560

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1620

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1680

gacagcuaca ucgugaucgg cgugggcgac aagaagauca cccaccacug gcacagaagc     1740

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1800

cugggcgaca ccgccuggga cuucggcagc gugggcggcg uguucaacag ccugggcaag     1860

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1920

agccagaucc ugaucggcac ccugcuggug uggcugggcc ugaacaccaa gaacggcagc     1980

aucagccuga ccugccuggc ccugggcggc gugaugaucu uccugagcac cgccgugagc     2040

gcc                                                                   2043


<210>  2138
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short CDS opt

<400>  2138
accagcaucg gcaucaucgg ccugcugcug accaccgcca uggccgccga gaucaccaga       60

agaggcagcg ccuacuacau guaccuggac agaagcgacg ccggcaaggc caucagcuuc      120

gccaccaccc ugggcgugaa caagugccac gugcagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgagacca gaagaagcag aagaagcgug agccugagau accacuacac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca agaagcaccu gaucauggug      420

gagaacugga ucuucagaaa ccccggcuuc gccaucguga gcguggccau caccuggcug      480

augggcagcc ugaccagcca gaaggugauc uaccugguga ugaucgugcu gaucgugccc      540

gccuacagca ucagcugcau cggcgugagc aacagagacc ugguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgagau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc augaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag ccugagcgac auggccagcg ccagcagaug ccccacccag      780

ggcgagccca gccuggacaa gcagagcgac acccagagcg ugugcaagag aacccugggc      840

gacagaggcu ggggcaacgg cugcggcauc uucggcaagg gcagccuggu gaccugcagc      900

aaguucaccu gcugcaagaa gaugcccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugcccgugca cggcagccag cacagcggca ugaucgugaa cgacaucggc     1020

cacgagaccg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc agagcgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2139
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short CDS opt

<400>  2139
agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga       60

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      120

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      180

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      240

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      300

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      360

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      420

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      480

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      540

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      600

accugggugg acguggugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      660

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      720

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      780

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      840

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      900

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga      960

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1020

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1080

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1140

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1200

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1260

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1320

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1380

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1440

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1500

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1560

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1620

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1680

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1740

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1800

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1860

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1920

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     1980

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2040

agcaccgccg ugagcgcc                                                   2058


<210>  2140
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short CDS opt

<400>  2140
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccagaagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2141
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short CDS opt

<400>  2141
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga uguucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2142
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short CDS opt

<400>  2142
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaugac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgc c                                               2061


<210>  2143
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short CDS opt

<400>  2143
accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga       60

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      120

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      180

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      240

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      300

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      360

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      420

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      480

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      540

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      600

ggcaccuggg uggacguggu gcuggagcac ggcggcugcg ugaccgccau ggcccaggac      660

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      720

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      780

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      840

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      900

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac      960

agaaucaugc ugagcgugca cggcagccag cacagcggca ugcuggugaa cgacaccggc     1020

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggccca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgcca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1500

cccgccgaga ccguggacgg caccgugacc guggagggcc aguacggcgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1860

cugaacagcc ugggcaaggg cauccaccag aucaucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     1980

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2040

cugagcaccg ccgugagcgg c                                               2061


<210>  2144
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short CDS opt

<400>  2144
ggcgccgacg ccagcaucgg caucgugagc cugcugcuga ccaccgugau ggccgccgag       60

aucaccagaa gaggcagcgc cuacuacaug uaccuggaca gaagcgacgc cggcaaggcc      120

aucagcuucg ugaccacccu gggcgugaac aagugccacg ugcagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc      360

agaaagcugc agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug      420

aucaaggugg agaacuggau cuucagaaac cccggcuucg cccugguggc cguggccauc      480

gccuggcugc ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc      600

augagcggcg gcaccugggu ggacguggug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugagaa gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga      840

acccuggugg acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucaccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguaca gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacgagaaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1080

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1140

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1200

aucccccugc ccuggcacag cggcgccgac accgagaccc cccacuggaa caacaaggag     1260

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1320

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1380

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1440

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1500

acccugcacg gcaccgugac cguggaggug caguacgccg gcagagacgg ccccugcaag     1560

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1620

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1680

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1740

cacagaagcg gcagcaucau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1800

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1860

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1920

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     1980

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2040

gccgugagcg cc                                                         2052


<210>  2145
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short CDS opt

<400>  2145
ggcgccgaca ccagcaucgg caucaucggc cugcugcuga ccaccgccau ggccgccgag       60

aucaccagaa gaggcagcgc cuacuacaug uaccuggaca gaagcgacgc cggcaaggcc      120

aucagcuucg ccaccaccau gggcgugaac aagugccacg ugcagaucau ggaccugggc      180

cacaugugcg acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc      240

gacgacgugg acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac      300

cacaagaagg gcgaggccag acccagcaga agagccguga cccugcccag ccacagcacc      360

agaaagcugc agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug      420

aucaaggugg agaacuggau ccugagaaac cccggcuuca ccguggccgc ccuggccaug      480

gccuggcugc ugggcaacag caccagccag agagugaucu accuggugau gauccugcug      540

aucgcccccg ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc      600

augagcggcg gcaccugggu ggacguggug cuggagcacg gcggcugcgu gaccgugaug      660

gcccaggaca agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc      720

gaggugagaa gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc      780

cccacccagg gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga      840

acccuggugg acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug      900

accugcgcca aguucaccug cagcaagaag augaccggca agagcaucca gcccgagaac      960

cuggaguaca gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac     1020

gacaucggcc acgagaccga cgagaacaga gccaaggugg aggugacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug uucagcggcc accugaagug cagaaucaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

agcaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

agagacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccauc aucaccgaga gcaccgagaa cagcagaaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     1740

aagaucaccc accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugagaggcg ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgugu ucaacagccu gggcaagggc gugcaccagg uguucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcuggugugg     1980

cugggccuga acaccaagaa cggcagcauc agccuggccu gccuggcccu gggcggcgcc     2040

augaucuucc ugagcaccgc cgugagcgcc                                      2070


<210>  2146
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE CDS opt

<400>  2146
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2147
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE CDS opt

<400>  2147
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2148
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE CDS opt

<400>  2148
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga cagagccaag      540

guggagguga cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug      600

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug      660

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac      720

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag      780

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac      840

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc      900

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc      960

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1020

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1080

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1140

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1200

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1260

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1320

accgccuggg acuucggcag c                                               1341


<210>  2149
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE CDS opt

<400>  2149
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2150
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE CDS opt

<400>  2150
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggcuacga gaccgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

aagcuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caagaucccc     1080

gugcagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2151
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE CDS opt

<400>  2151
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugacc gugaacgaca ucggcuacga gaccgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

aagcuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caagaucccc     1080

gugcagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2152
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE CDS opt

<400>  2152
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgacg agaacagagc caagguggag      540

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug      600

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac      660

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc      720

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc      780

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc      840

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac      900

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc      960

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1020

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1080

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1140

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1200

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1260

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1320

ugggacuucg gcagc                                                      1335


<210>  2153
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE CDS opt

<400>  2153
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagaa cagagccaag      540

guggagguga cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug      600

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug      660

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac      720

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag      780

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac      840

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcaa gcuguucagc      900

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc      960

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1020

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1080

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1140

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1200

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1260

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1320

accgccuggg acuucggcag c                                               1341


<210>  2154
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE CDS opt

<400>  2154
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagccugaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2155
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE CDS opt

<400>  2155
agcaagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2156
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE CDS opt

<400>  2156
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cugguacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2157
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE CDS opt

<400>  2157
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

aagcugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2158
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE CDS opt

<400>  2158
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacac ccagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2159
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE CDS opt

<400>  2159
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgaga ucagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2160
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE CDS opt

<400>  2160
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccag aagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2161
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE CDS opt

<400>  2161
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggu gagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2162
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE CDS opt

<400>  2162
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcau cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2163
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE CDS opt

<400>  2163
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcaucggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2164
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE CDS opt

<400>  2164
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccuggccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2165
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE CDS opt

<400>  2165
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cacccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2166
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE CDS opt

<400>  2166
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugcug     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2167
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE CDS opt

<400>  2167
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggaggccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2168
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE CDS opt

<400>  2168
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccgc ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2169
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE CDS opt

<400>  2169
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugagcaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccaccauca gcgacaucgc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgccg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2170
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE CDS opt

<400>  2170
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucagca agauccccgc cgagacccug     1020

cacggcaccg ugaccgagga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2171
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE CDS opt

<400>  2171
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga gcugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2172
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE CDS opt

<400>  2172
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggca ucagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcaucc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2173
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE CDS opt

<400>  2173
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgugaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2174
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE CDS opt

<400>  2174
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcgccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2175
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE CDS opt

<400>  2175
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acaccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagggcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2176
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE CDS opt

<400>  2176
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gccauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugcug gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gcccacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgccaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagaccgug     1020

gacggcaccg ugaccgugga gggccaguac ggcggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2177
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE CDS opt

<400>  2177
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc cgccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2178
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE CDS opt

<400>  2178
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugaacaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2179
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE CDS opt

<400>  2179
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcgc cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2180
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE CDS opt

<400>  2180
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2181
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE CDS opt

<400>  2181
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cugguacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2182
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE CDS opt

<400>  2182
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2183
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE CDS opt

<400>  2183
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2184
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE CDS opt

<400>  2184
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgugugc accgccgcca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2185
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE CDS opt

<400>  2185
agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgacg agaacagagc caagguggag      540

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug      600

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac      660

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacagcggc      720

gccgacaccg agacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc      780

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc      840

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac      900

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc      960

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1020

gaggugcagu acgccggcag agacggcccc ugcaaggugc ccgcccagau ggccguggac     1080

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1140

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1200

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caucaucggc     1260

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1320

ugggacuucg gcagc                                                      1335


<210>  2186
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE CDS opt

<400>  2186
agccagaagg ugaucuaccu ggugaugauc gugcugaucg ugcccgccua cagcaucagc       60

ugcaucggcg ugagcaacag agaccuggug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gagauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaugac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagccuga gcgacauggc cagcgccagc agaugcccca cccagggcga gcccagccug      300

gacaagcaga gcgacaccca gagcgugugc aagagaaccc ugggcgacag aggcuggggc      360

aacggcugcg gcaucuucgg caagggcagc cuggugaccu gcagcaaguu caccugcugc      420

aagaagaugc ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugccc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcagagc gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2187
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE CDS opt

<400>  2187
agacagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      540

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2188
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE CDS opt

<400>  2188
agccagagag ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga       60

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      120

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      180

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      240

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      300

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      360

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      420

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      480

gugcacggca gccagcacag cggcaugauc gugaacgaca ucggccacga gaccgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacuguuca gcggccaccu gaagugcaga aucaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucagca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcagag acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccaucauca ccgagagcac cgagaacagc agaaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agc                                  1353


<210>  2189
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  2189
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaau auggccgaag ucagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguugaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcgca cgcuaagaga cagacggugg ucgugcucgg cucucaggag     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccgugauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcgugcuca ucuuccugag caccgcuguu     2100

ucugccgacg uggggugcuc cgucgauuuu agcaaa                               2136


<210>  2190
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS opt

<400>  2190
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca caugugacgc caccaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

acaaccucca cgugggucgu guauggaacc ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacacu ucccagccau uccacccgca agcugcaaac aagaucucag      420

accuggcucg agagccggga auacacgaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcaca      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac cugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggcuc aggauaagcc uaccguggac      720

auugagcuug ugaccacaac ggucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cccaaggaga agccuaccug      840

gacaaacagu cugauacaca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggucacau gcgcuaaguu ugcgugcucc      960

aagaaaauga ccggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

gugcacggcu cccaacacag cgggaugauc guuaacgaua cgggacauga gacagacgag     1080

aauagagcca aaguggaaau uaccccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggacaggcc uggacuuuuc cgaucuuuau     1200

uaccugacca ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauacagga acgccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagaccgucg ugguucucgg cucucaggag     1380

ggggccgugc auacagcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac cgcggccuuc acauuuacca aaauuccggc ugaaacgcug     1560

cauggcaccg ugacaguuga ggugcaguac gccgggaccg acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacacug accccugugg gccgccucau cacggcgaac     1680

cccguuauca cagaauccac cgagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acacagccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacgcuccug auguggcugg gccucaaugc caagaacggg     2040

agcaucuccc ugaugugucu ugcucuggga ggcguucuca uuuuccugag caccgccgug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2191
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS opt

<400>  2191
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aacccuggga      180

gugaauaaau gccacgucca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cggugacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauua aaguugaaaa uuggaucuuu      480

agaaaccccg gcuucgcccu gguggcuguc gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaagg ugaucuaccu uguuaugauu cugcucaucg ccccugcgua uagcauucgg      600

ugcaucggag ugucuaaucg cgauuuuguc gagggcaugu ccgggggaac auggguggac      660

gugguccugg aacacggcgg guguguuacc gugauggccc aggauaaacc cacaguggac      720

aucgagcuug ucaccaccac agugagcaac auggcugagg uuagaucuua cugcuacgaa      780

gccuccauua gcgauauggc uuccgacucu cgguguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgugugc aaaagaacac ucgucgaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cuggugaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauccagccc gaaaaccucg aguaucggau uaugcugucc     1020

guucacggca gccaacauuc ugggaugauc ggauacgaaa cggaugagga cagagcuaag     1080

guggagguca caccuaauag cccgcgcgcg gaagccaccc uuggcggcuu cgggucccug     1140

ggacucgauu gugagccccg gaccggccug gacuuuucug aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccucu gcccuggcac     1260

gcuggggccg auaccggaac accucauugg aacaauaaag aggcccuggu cgaauuuaag     1320

gacgcucacg ccaagagaca gacggugguu gugcucggca gccaggaggg ggcgguccau     1380

accgcucugg ccggcgcccu ugaggcugaa auggauggag ccaaaggccg ccuguucucc     1440

gggcaccuca agugccggcu gaaaauggac aaacuuagac ugaagggagu gagcuauucu     1500

cucuguacag cugccuuuac cuucacaaag guucccgcgg agacccugca uggcacggug     1560

accgucgaag ugcaguacgc cgggacagac ggaccgugca agaucccugu gcaaauggcu     1620

guugauaugc agacccugac acccgucggc cgccucauca ccgccaaccc ugugauuacg     1680

gaguccacag aaaauagcaa aaugaugcug gagcuugacc cucccuuugg ggauucuuac     1740

aucgugauug gcgucggaga caagaaaauc acccaccacu ggcaucgguc cggcagcaca     1800

auugggaagg cuuucgaggc caccgugaga ggagcgaaac gcauggccgu ucugggcgau     1860

accgcuuggg acuucggguc cgugggaggc gucuuuaacu cucucgggaa gggcauccac     1920

cagaucuucg gagccgcuuu uaagagccug uucggcggga uguccugguu uucucaaauu     1980

cugaucggaa cacuucucgu guggcugggc cugaacacga aaaaugggag cauuucccuc     2040

accugucugg cccuuggagg cguuaugauc uuccugagca cagccguguc ugcugauguc     2100

gggugcuccg uggacuuuag caag                                            2124


<210>  2192
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) CDS opt

<400>  2192
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2193
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  2193
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc c                                               2061


<210>  2194
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) CDS opt

<400>  2194
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacacaugu      180

gaugccacca ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacacaac cuccacgugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acacuuccca gccauuccac ccggaaacug      360

caaacacgcu cucagaccug gcucgagagc agagaguaca cgaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacaucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggaccuggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcgcaggac      660

aagccuaccg uggauauuga gcuugugacc acaacggucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacccaa      780

ggggaggcuu accuggacaa gcagucugac acacaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu cacaugcgcc      900

aaauuugccu gcuccaagaa aaugaccggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacacgggg     1020

caugaaacag augagaaccg ggcuaaggug gagauuaccc ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac aggacuggau     1140

uuuuccgacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca cagggacgcc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac cgucgugguu     1320

cucggaucuc aggagggcgc ggugcauaca gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguaccgcug ccuucacauu uaccaagauu     1500

ccggcggaga cgcugcaugg aaccgugaca guugaagugc aguacgccgg caccgacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacgg cuaaccccgu uauuacagag uccaccgaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauaca gccugggacu ucggcuccgu ugggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacgc uccugaugug gcugggacuc     1980

aacgcuaaga auggcagcau cucccugaug ugucuugcgc uugggggagu gcucauuuuc     2040

cugagcaccg ccgugucugc u                                               2061


<210>  2195
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) CDS opt

<400>  2195
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gaucaagguu      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uucgcugcau cggggugucu aacagagacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcac ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgg      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaaag aacacucguc      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcauuaugc uguccguuca cggaagccaa cauucuggca ugaucgggua cgagacggac     1020

gaagauagag ccaaggugga ggucacaccu aacagcccgc gggccgaggc uacccuugga     1080

ggcuucggcu cccuggggcu cgacugugaa cccagaaccg gacuggauuu uucugaccuu     1140

uauuaccuga caaugaauaa caaacacugg cucgugcaua aggagugguu ccacgauauu     1200

ccucugcccu ggcacgcggg cgccgacacc gggacaccuc auuggaauaa caaagaagcu     1260

cuggucgagu uuaaggaugc ccacgccaaa cgccagacgg ugguugugcu cggaagccag     1320

gaaggcgcug uccauaccgc ccuggcgggg gcucuugagg ccgagaugga cggcgccaag     1380

ggacggcugu ucuccggcca ccucaagugc agacugaaaa uggauaaacu ucgccugaag     1440

ggggugagcu auucucucug uacagcugcc uuuaccuuca caaagguucc cgcugaaacc     1500

cugcauggaa cggugaccgu cgaggugcag uacgccggca cagacgggcc gugcaaaauc     1560

ccugugcaaa uggcgguuga caugcagacc cugacacccg ucggacggcu cauuaccgcc     1620

aauccuguga ucacggaauc cacagagaac agcaagauga ugcuggaacu ugauccuccc     1680

uuuggcgacu cuuacaucgu gauugggguc ggcgauaaga aaaucaccca ccacuggcau     1740

agauccggaa gcacaauugg caaggcuuuc gaggccaccg ugcgcggggc uaaacggaug     1800

gccguucugg gagacaccgc gugggauuuc ggcuccgucg ggggaguguu uaauucucuc     1860

ggcaagggga uccaccagau uuucggcgcc gcuuuuaaaa gccuguucgg aggcaugucc     1920

ugguuuucuc aaauccugau cgggacacuu cucguguggc ugggacugaa cacgaagaac     1980

ggcagcauuu cccucaccug ucuggcccuu gggggaguua ugaucuuccu gagcacagcu     2040

gugucugcc                                                             2049


<210>  2196
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) CDS opt

<400>  2196
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2197
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS opt

<400>  2197
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

auuguccuug aacacggggg augugugaca guuauggccc aagacaaacc uacgguggau      180

aucgagcugg ucaccacaac cguguccaau auggcggaag uucgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugugugc aaacggacac uggucgaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauucaaccc gagaaucucg aguacagaau caugcugagc      480

gugcaugggu cucagcacuc cggaaugauu gucaacgaua ccggccauga aacagacgag      540

aaucgggcua agguugaaau caccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacauuccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

guggaguuca aggaugcuca cgccaagcgg cagacagucg ugguucucgg gagccaggaa      840

ggagcggugc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gucagcuacu cccugugcac cgccgcuuuu acauucacga agauccccgc ggagacccuu     1020

cacgggacag ugaccguuga agugcaauau gccggaacag acggcccuug uaaagucccc     1080

gcucagaugg ccguggauau gcagacccug acgccugucg ggcgccucau uacagccaau     1140

cccgugauca ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucguuau uggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

agcggcucua ccauuggaaa ggcuuucgag gccacagucc ggggcgcgaa acgcauggcu     1320

gugcucgggg acacggccug ggauuuugga agc                                  1353


<210>  2198
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS opt

<400>  2198
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg acacggccug ggauuuugga agc                                  1353


<210>  2199
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS opt

<400>  2199
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc ggcuaugaaa cagaugagga ccgggccaag      540

guugaaguga ccccuaacag cccccgcgcu gaggccacac ucgggggauu uggcucucug      600

ggccuggauu gcgagccuag aaccgggcuu gacuucuccg aucuguacua ccucacgaug      660

aauaacaaac auuggcuggu gcacaaggaa ugguuucaug acauuccccu uccuuggcac      720

gcgggagccg acacaggcac cccgcacugg aauaacaaag aggcucuggu cgaguucaag      780

gaugcccaug cuaagcggca gacagugguu gugcucggga gccaggaagg agccguccac      840

accgcgcugg ccggcgcucu ggaagccgag auggacgggg cuaaaggacg ccucuuuucc      900

ggccaucuga aguguagacu uaaaauggau aagcugcggc ucaaaggcgu gucuuauagc      960

cugugcaccg ccgccuucac auuuacgaag guucccgcug agacccuuca cgggacagug     1020

accgucgaag ugcaauacgc gggaacagac ggcccuugua aaauccccgu ccagauggcc     1080

guggauaugc agacccugac gccuguuggg cgccucauua cagcuaaucc cgugaucacc     1140

gaguccaccg aaaacagcaa gaugaugcug gagcuggacc cgccuuucgg agauucuuau     1200

auugucaucg gcguggggga caagaaaauc acacaccauu ggcacagauc cggcagcacc     1260

auuggaaagg ccuuugaagc cacaguucgg ggcgcuaaac gcauggccgu gcucggggau     1320

acggcguggg acuucggauc u                                               1341


<210>  2200
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) CDS opt

<400>  2200
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg acacggccug ggauuuugga agc                                  1353


<210>  2201
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) CDS opt

<400>  2201
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagccuc uauuuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug agcggaaagg gcucccuggu gaccugcgcc      900

aaauucgccu gcagcaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc uguccgucca cggaagccaa cacucuggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac cgaauagccc cagagcggaa     1080

gccacccuug gaggcuuugg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uucucugacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuucacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga guucaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaagcc aggaaggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aagggguguc uuauucccuc uguacagcug ccuuuaccuu cacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag agcacagaaa acucuaagau gaugcuggag     1680

cuugaucccc cuuuuggcga cuccuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcagcgg auccacaauu ggcaaggccu ucgaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu uuggcucugu cgggggagcu     1860

cucaauagcc ugggcaaggg gauccaccag auuuucggcg ccgccuucaa aucccuguuu     1920

ggaggcaugu cuugguucag ccaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcuccau uagccugaug ugucuugcuc uugggggagu gcucaucuuu     2040

cugucuaccg cgguuuccgc c                                               2061


<210>  2202
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) CDS opt

<400>  2202
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacacaugu      180

gaugccacca ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacacaac cuccacgugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acacuuccca gccauuccac ccggaaacug      360

caaacacgcu cucagaccug gcucgagagc agagaguaca cgaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacaucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggaccuggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcgcaggac      660

aagccuaccg uggauauuga gcuugugacc acaacggucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacccaa      780

ggggaggcuu accuggacaa gcagucugac acacaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug agcggaaagg gcucccuggu cacaugcgcc      900

aaauucgccu gcagcaagaa aaugaccggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc uguccgugca cggaagccaa cacucuggca ugaucguuaa ugacacgggg     1020

caugaaacag augagaaccg ggcuaaggug gagauuaccc cgaauagccc cagagcggaa     1080

gccacccuug gaggcuuugg cucccugggg cucgacugug agccucgcac aggacuggau     1140

uucucugacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuucacgaua ucccccugcc uuggcacgcu ggcgccgaca cagggacgcc ccauuggaac     1260

aauaaagagg cccuggugga guucaaggau gcucacgcca aacggcagac cgucgugguu     1320

cucggaagcc aggaaggcgc ggugcauaca gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucuc uuauucccuc uguaccgcug ccuuuacauu caccaagauu     1500

ccggcggaga cgcugcaugg aaccgugaca guugaagugc aguacgccgg caccgacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacgg cuaaccccgu uauuacagag agcaccgaaa acucuaagau gaugcuggag     1680

cuugaucccc cuuuuggcga cuccuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcagcgg auccaccauc ggcaaggccu ucgaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauaca gccugggacu uuggcucugu ugggggagcu     1860

cucaauagcc ugggcaaggg gauucaccag aucuucggcg ccgccuucaa aucccuguuu     1920

ggaggcaugu cuugguucag ccaaauucuu aucgggacgc uccugaugug gcugggacuc     1980

aacgcuaaga auggcuccau cagccugaug ugucuugcgc uugggggagu gcucauuuuu     2040

cugucuaccg ccguguccgc u                                               2061


<210>  2203
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) CDS opt

<400>  2203
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gaucaagguu      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uucgcugcau cggggugucu aacagagacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcac ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgg      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaaag aacacucguc      840

gaucggggau ggggcaacgg cugugggcug uccggaaagg gcagccuggu gaccugcgcu      900

aaauucacau gcucuaagaa gaugaccggg aaauccaucc agcccgagaa ucucgaauau      960

cgcauuaugc ugagcguuca cggaucucaa cauagcggca ugaucgggua cgagacggac     1020

gaagauagag ccaaggugga ggucacaccu aacuccccgc gggccgaggc uacccuugga     1080

ggcuuuggcu cucuggggcu cgacugugaa cccagaaccg gacuggauuu cagcgaccuu     1140

uauuaccuga caaugaauaa caaacacugg cucgugcaua aggagugguu ucacgauauu     1200

ccucugcccu ggcacgcggg cgccgacacc gggacaccuc auuggaauaa caaagaagcu     1260

cuggucgagu ucaaggaugc ccacgccaaa cgccagacgg ugguugugcu cggaucccag     1320

gaaggcgcug uccauaccgc ccuggcgggg gcucuugagg ccgagaugga cggcgccaag     1380

ggacggcugu uuagcggcca ccucaagugc agacugaaaa uggauaaacu ucgccugaag     1440

ggggugucuu auucccucug uacagcugcc uucaccuuua caaagguucc cgcugaaacc     1500

cugcauggaa cggugaccgu cgaggugcag uacgccggca cagacgggcc gugcaaaauc     1560

ccugugcaaa uggcgguuga caugcagacc cugacacccg ucggacggcu cauuaccgcc     1620

aauccuguga ucacggaaag cacagagaac ucuaagauga ugcuggaacu ugauccuccc     1680

uucggcgacu ccuacaucgu gauugggguc ggcgauaaga aaaucaccca ccacuggcau     1740

agaagcggau ccacaauugg caaggcuuuu gaggccaccg ugcgcggggc uaaacggaug     1800

gccguucugg gagacaccgc gugggauuuc ggcucugucg ggggaguguu caauagccuc     1860

ggcaagggga uccaccagau uuuuggcgcc gcuuucaaau cccuguuugg aggcaugucu     1920

ugguucagcc aaauccugau cgggacacuu cucguguggc ugggacugaa cacgaagaac     1980

ggcuccauua gccucaccug ucuggcccuu gggggaguua ugaucuuucu gucuacagcu     2040

guguccgcc                                                             2049


<210>  2204
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) CDS opt

<400>  2204
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug agcggaaagg gcucccuggu gaccugcgcc      900

aaauucgccu gcagcaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc uguccgugca cggaagccaa cacucuggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac cgaauagccc cagagcggaa     1080

gccacccuug gaggcuuugg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uucucugacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuucacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga guucaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaagcc aggaaggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucuc uuauucccuc uguacagcug ccuuuaccuu cacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag agcacagaaa acucuaagau gaugcuggag     1680

cuugaucccc cuuuuggcga cuccuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcagcgg auccacaauc ggcaaggccu ucgaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu uuggcucugu cgggggagcu     1860

cucaauagcc ugggcaaggg gauucaccag aucuucggcg ccgccuucaa aucccuguuu     1920

ggaggcaugu cuugguucag ccaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcuccau cagccugaug ugucuugcuc uugggggagu ucucauuuuu     2040

cugucuaccg cgguguccgc c                                               2061


<210>  2205
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) CDS opt

<400>  2205
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugca ggacaccggg     1020

caugaaacgg augagaaucg ggcuaagguu gagaucacac cgaacucucc cagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaau aacaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaau     1260

aacaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aauacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca gauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaucccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaacucuc ugggcaaggg gauccaccaa auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucagauccuu aucgggacac uccugaugug gcugggacuc     1980

aauacgaaga acggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc c                                               2061


<210>  2206
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) CDS opt

<400>  2206
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacacaugu      180

gaugccacca ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacacaac cuccacgugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acacuuccca gccauuccac ccggaaacug      360

caaacacgcu cucagaccug gcucgagagc agagaguaca cgaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacaucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggaccuggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcgcaggac      660

aagccuaccg uggauauuga gcuugugacc acaacggucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacccaa      780

ggggaggcuu accuggacaa gcagucugac acacaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu cacaugcgcc      900

aaauuugccu gcuccaagaa aaugaccggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuca ggacacgggg     1020

caugaaacag augagaaucg ggcuaaggug gagauuaccc cgaacucucc cagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac aggacuggau     1140

uuuuccgacc uuuauuaccu gaccaugaau aacaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca cagggacgcc ccauuggaau     1260

aacaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac cgucgugguu     1320

cucggaucuc aggagggcgc ggugcauaca gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguaccgcug ccuucacauu uaccaagauu     1500

ccggcggaga cgcugcaugg aaccgugaca guugaagugc aauacgccgg caccgacggg     1560

ccuugcaaag uccccgcuca gauggccgug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacgg cuaaucccgu uauuacagag uccaccgaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauaca gccugggacu ucggcuccgu ugggggagcu     1860

cucaacucuc ugggcaaggg gauucaccaa aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucagauucuu aucgggacgc uccugaugug gcugggacuc     1980

aaugcuaaga acggcagcau cucccugaug ugucuugcgc uugggggagu gcucauuuuc     2040

cugagcaccg ccgugucugc u                                               2061


<210>  2207
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) CDS opt

<400>  2207
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuca ggacaccggg     1020

caugaaacgg augagaaucg ggcuaaggug gagauuacac cgaacucucc cagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaau aacaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaau     1260

aacaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aauacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca gauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaucccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaacucuc ugggcaaggg gauucaccaa aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucagauucuu aucgggacac uccugaugug gcugggacuc     1980

aauacgaaga acggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2208
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2208
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaaa     1440

cuucggcuga agggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacacug ggcaaggccu uuuccaccac ccucaaaggg     1800

gcgcagcggc uggccgcucu gggagauaca gccugggacu ucggcucuau ugggggagug     1860

uucaauagca ucggcagagc ugugcaccaa gucuuugggg gcgccuuccg cacgcuuuuu     1920

ggaggcaugu ccuggauuac ccaggggcuc augggagccc ugcugcucug gaugggcgug     1980

aacgcucggg auagaucuau cgcgcuggcc uuccuugcua cagggggagu ucugguguuu     2040

cucgccacca auguccaugc c                                               2061


<210>  2209
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2209
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacacaugu      180

gaugccacca ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacacaac cuccacgugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acacuuccca gccauuccac ccggaaacug      360

caaacacgcu cucagaccug gcucgagagc agagaguaca cgaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacaucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggaccuggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcgcaggac      660

aagccuaccg uggauauuga gcuugugacc acaacggucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacccaa      780

ggggaggcuu accuggacaa gcagucugac acacaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu cacaugcgcc      900

aaauuugccu gcuccaagaa aaugaccggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacacgggg     1020

caugaaacag augagaaccg ggcuaaggug gagauuaccc ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac aggacuggau     1140

uuuuccgacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca cagggacgcc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac cgucgugguu     1320

cucggaucuc aggagggcgc ggugcauaca gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaaa     1440

cuucggcuga agggggucag cuauucucuc uguaccgcug ccuucacauu uaccaagauu     1500

ccggcggaga cgcugcaugg aaccgugaca guugaagugc aguacgccgg caccgacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacgg cuaaccccgu uauuacagag uccaccgaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcacccug ggcaaggccu uuuccaccac acucaaaggg     1800

gcgcagcggc uggccgcucu gggagauacg gccugggacu ucggcucuau cgggggagug     1860

uucaauagca uuggcagagc uguccaccaa guguuugggg gcgccuuccg cacccuuuuu     1920

ggaggcaugu ccuggaucac acaggggcuc augggagccc ugcugcucug gaugggcguu     1980

aacgcucggg auagaucuau ugcgcuggcc uuccuugcua ccgggggagu gcuggucuuu     2040

cucgccacaa augugcaugc c                                               2061


<210>  2210
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       CDS opt

<400>  2210
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gaucaagguu      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uucgcugcau cggggugucu aacagagacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcac ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgg      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaaag aacacucguc      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcauuaugc uguccguuca cggaagccaa cauucuggca ugaucgggua cgagacggac     1020

gaagauagag ccaaggugga ggucacaccu aacagcccgc gggccgaggc uacccuugga     1080

ggcuucggcu cccuggggcu cgacugugaa cccagaaccg gacuggauuu uucugaccuu     1140

uauuaccuga caaugaauaa caaacacugg cucgugcaua aggagugguu ccacgauauu     1200

ccucugcccu ggcacgcggg cgccgacacc gggacaccuc auuggaauaa caaagaagcu     1260

cuggucgagu uuaaggaugc ccacgccaaa cgccagacgg ugguugugcu cggaagccag     1320

gaaggcgcug uccauaccgc ccuggcgggg gcucuugagg ccgagaugga cggcgccaag     1380

ggacggcugu ucuccggcca ccucaagugc agacugaaaa uggauaaacu ucgccugaag     1440

ggggugagcu auucucucug uacagcugcc uuuaccuuca caaagguucc cgcugaaacc     1500

cugcauggaa cggugaccgu cgaggugcag uacgccggca cagacgggcc gugcaaaauc     1560

ccugugcaaa uggcgguuga caugcagacc cugacacccg ucggacggcu cauuaccgcc     1620

aauccuguga ucacggaauc cacagagaac agcaagauga ugcuggaacu ugauccuccc     1680

uuuggcgacu cuuacaucgu gauugggguc ggcgauaaga aaaucaccca ccacuggcau     1740

agauccggaa gcacacuggg caaggcuuuc uccaccaccc ucaaaggggc ccagcgccug     1800

gcugcccugg gagacacagc gugggauuuc ggcucuauug ggggaguguu uaauagcauc     1860

ggccgggccg uucaccaagu guucgggggc gcuuuuagaa cgcuuuucgg aggcaugucc     1920

uggauuaccc aggggcucau gggagcccug cugcucugga ugggcgucaa cgcucgcgac     1980

cggucuaucg cccuggccuu ucuugcuaca gggggagugc ugguuuuccu cgcgaccaac     2040

gugcaugcc                                                             2049


<210>  2211
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2211
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaaa     1440

cuucggcuga agggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacacug ggcaaggccu uuuccaccac ccucaaaggg     1800

gcgcagcggc uggccgcucu gggagauaca gccugggacu ucggcucuau cgggggagug     1860

uucaauagca uuggcagagc uguccaccaa guguuugggg gcgccuuccg cacgcuuuuu     1920

ggaggcaugu ccuggaucac ccaggggcuc augggagccc ugcugcucug gaugggcguu     1980

aacgcucggg auagaucuau ugcgcuggcc uuccuugcua cagggggagu gcuggucuuu     2040

cucgccacca augugcaugc c                                               2061


<210>  2212
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  2212
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaau auggccgaag ucagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguugaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcgca cgcuaagaga cagacggugg ucgugcucgg cucucaggag     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccgugauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcgugcuca ucuuccugag caccgcuguu     2100

ucugccgacg uggggugcuc cgucgauuuu agcaaa                               2136


<210>  2213
<211>  2133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2213
aucauuaacg ccagaaagga gaaaaagcgg cgcggcgcug acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc cauggcggcu gaaguuacca gacggggcuc cgccuacuau      120

auguaccuug aucgcaauga cgccggggag gcuauuucuu uccccacaac ccugggaaug      180

aacaaaugcu auauccagau uauggaucuc ggccacaugu gugacgccac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaauacc      300

acauccaccu gggucgugua uggaacgugu caucacaaga aaggcgaggc uagacggucu      360

cgcagagccg uuacccuucc cagccauucc acacggaagc ugcaaacccg cucucagaca      420

uggcucgaga gcagagaaua caccaagcac cugauccggg uggagaacug gaucuuucgc      480

aauccuggcu ucgcgcuggc cgcugccgcu auugccuggc uccugggguc cagcacgucu      540

cagaaaguca ucuaucuugu gaugauucug cucaucgcgc ccgccuacuc cauuagaugc      600

aucggaguua gcaaccggga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcucagg auaagccuac aguugacauc      720

gagcuuguga ccaccacagu guccaauaug gccgaagucc gcagcuacug cuaugaggcu      780

ucuaucuccg auauggccag cgacuccaga ugucccacgc aaggagaagc cuaccuggac      840

aaacagucug auacccagua ugugugcaag cggacacucg uugacagagg cuggggcaac      900

ggguguggac uguucggcaa agggagccug gugaccugcg cuaaguuugc gugcuccaag      960

aaaaugacag gaaagagcau ucagccugag aaucucgaau accgcaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aacgauaccg gacaugagac ggacgagaau     1080

cgggccaaag uugaaaucac accgaacucu cccagagcug aggccacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccucgc accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga auaacaagca cuggcucgug cauaaagagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggcuga uaccggaaca ccccauugga auaacaagga agcccugguc     1320

gaguuuaaag acgcgcacgc uaagcggcag acgguggucg ugcucggcuc ucaggagggg     1380

gccguucaua ccgcccuggc uggcgcccuu gaagcugaga uggauggagc caagggcaga     1440

cugagcuccg ggcaccucaa augccgccug aagauggaca aacuucggcu gaagggagug     1500

agcuauucuc ucuguacagc ggccuucacc uuuacaaaaa ucccggcuga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcc gggacagacg gaccuugcaa ggugcccgcu     1620

caaauggccg ucgauaugca gacccugaca ccugugggca gacucaucac cgcgaacccc     1680

gugauuacgg aauccacaga gaauagcaag augaugcugg agcuugaucc cccuuucggg     1740

gacucuuaca ucguuauugg cgucggagag aaaaagauca cccaccacug gcaucgcucc     1800

ggcagcacaa uugggaaagc cuuugaagcu accgugcggg gagccaagag aauggcugug     1860

cugggcgaca ccgccuggga uuucgggucc gucggaggcg cccucaacuc ucuggggaaa     1920

ggcauccacc agaucuucgg agcugcguuu aagagccugu ucggcgggau guccugguuu     1980

ucucaaauuc uuaucggaac acuccugaug uggcugggcc ucaauacgaa gaacgggagc     2040

auuucccuga ugugucuugc ccugggaggc gugcucaucu uccugagcac cgcuguuucu     2100

gccgacgugg ggugcuccgu cgauuuuagc aaa                                  2133


<210>  2214
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2214
aucaacgcca gaaaggagaa aaagcggcgc ggcgcugaca ccagcguggg gauugucgga       60

cugcuccuga caacggccau ggcggcugaa guuaccagac ggggcuccgc cuacuauaug      120

uaccuugauc gcaaugacgc cggggaggcu aucucuuucc ccacaacccu gggaaugaac      180

aaaugcuaua uucagaucau ggaucucggc cacaugugug acgccacaau gagcuacgaa      240

ugcccuaugc uggaugaggg gguggaaccg gacgaugugg acuguuggug caauaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaagaaag gcgaggcuag acggucucgc      360

agagccguua cccuucccag ccauuccaca cggaagcugc aaacccgcuc ucagacaugg      420

cucgagagca gagaauacac caagcaccug auucgggugg agaacuggau cuuucgcaau      480

ccuggcuucg cgcuggccgc ugccgcuauc gccuggcucc ugggguccag cacgucucag      540

aaagucauuu aucuugugau gauccugcuc auugcgcccg ccuacuccau cagaugcauu      600

ggaguuagca accgggauuu uguggaaggc augucugggg gaacaugggu cgacaucgug      660

cuggagcaug gcgggugugu gaccgucaug gcucaggaua agccuacagu ugacauugag      720

cuugugacca ccacaguguc caauauggcc gaaguccgca gcuacugcua ugaggcuucu      780

aucuccgaua uggccagcga cuccagaugu cccacgcaag gagaagccua ccuggacaaa      840

cagucugaua cccaguaugu gugcaagcgg acacucguug acagaggcug gggcaacggg      900

uguggacugu ucggcaaagg gagccuggug accugcgcua aguuugcgug cuccaagaaa      960

augacaggaa agagcaucca gccugagaau cucgaauacc gcauuaugcu gucuguccac     1020

ggcucccaac acagcgggau gaucgugaac gauaccggac augagacgga cgagaaucgg     1080

gccaaaguug aaauuacacc gaacucuccc agagcugagg ccacccuugg cggcuucggg     1140

agccugggac ucgauuguga accucgcacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaua acaagcacug gcucgugcau aaagaguggu uccacgacau cccccugccu     1260

uggcacgccg gggcugauac cggaacaccc cauuggaaua acaaggaagc ccuggucgag     1320

uuuaaagacg cgcacgcuaa gcggcagacg guggucgugc ucggcucuca ggagggggcc     1380

guucauaccg cccuggcugg cgcccuugaa gcugagaugg auggagccaa gggcagacug     1440

agcuccgggc accucaaaug ccgccugaag auggacaaac uucggcugaa gggagugagc     1500

uauucucucu guacagcggc cuucaccuuu acaaaaauuc cggcugaaac ccugcauggc     1560

acggucaccg uggagguuca guacgccggg acagacggac cuugcaaggu gcccgcucaa     1620

auggccgucg auaugcagac ccugacaccu gugggcagac ucaucaccgc gaaccccgug     1680

aucacggaau ccacagagaa uagcaagaug augcuggagc uugauccccc uuucggggac     1740

ucuuacauug uuaucggcgu cggagagaaa aagauuaccc accacuggca ucgcuccggc     1800

agcacaaucg ggaaagccuu ugaagcuacc gugcggggag ccaagagaau ggcugugcug     1860

ggcgacaccg ccugggauuu cggguccguc ggaggcgccc ucaacucucu ggggaaaggc     1920

auucaccaga ucuucggagc ugcguuuaag agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua uuggaacacu ccugaugugg cugggccuca auacgaagaa cgggagcauc     2040

ucccugaugu gucuugcccu gggaggcgug cucauuuucc ugagcaccgc uguuucugcc     2100

gacguggggu gcuccgucga uuuuagcaaa                                      2130


<210>  2215
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2215
aacgccagaa aggagaaaaa gcggcgcggc gcugacacca gcguggggau cgucggacug       60

cuccugacaa cggccauggc ggcugaaguu accagacggg gcuccgccua cuauauguac      120

cuugaucgca augacgccgg ggaggcuauu ucuuucccca caacccuggg aaugaacaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg ccacaaugag cuacgaaugc      240

ccuaugcugg augagggggu ggaaccggac gauguggacu guuggugcaa uaccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aggcuagacg gucucgcaga      360

gccguuaccc uucccagcca uuccacacgg aagcugcaaa cccgcucuca gacauggcuc      420

gagagcagag aauacaccaa gcaccugauc cggguggaga acuggauuuu ucgcaauccu      480

ggcuucgcgc uggccgcugc cgcuaucgcc uggcuccugg gguccagcac gucucagaaa      540

gucaucuauc uugugaugau ucugcucauc gcgcccgccu acuccauuag augcaucgga      600

guuagcaacc gggauuuugu ggaaggcaug ucugggggaa caugggucga cauugugcug      660

gagcauggcg ggugugugac cgucauggcu caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa uauggccgaa guccgcagcu acugcuauga ggcuucuauu      780

uccgauaugg ccagcgacuc cagauguccc acgcaaggag aagccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagcggaca cucguugaca gaggcugggg caacgggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgcuaagu uugcgugcuc caagaaaaug      960

acaggaaaga gcauccagcc ugagaaucuc gaauaccgca ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaacgau accggacaug agacggacga gaaucgggcc     1080

aaaguugaaa ucacaccgaa cucucccaga gcugaggcca cccuuggcgg cuucgggagc     1140

cugggacucg auugugaacc ucgcaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaauaaca agcacuggcu cgugcauaaa gagugguucc acgacauucc ccugccuugg     1260

cacgccgggg cugauaccgg aacaccccau uggaauaaca aggaagcccu ggucgaguuu     1320

aaagacgcgc acgcuaagcg gcagacggug gucgugcucg gcucucagga gggggccguu     1380

cauaccgccc uggcuggcgc ccuugaagcu gagauggaug gagccaaggg cagacugagc     1440

uccgggcacc ucaaaugccg ccugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucucucugua cagcggccuu caccuuuaca aaaaucccgg cugaaacccu gcauggcacg     1560

gucaccgugg agguucagua cgccgggaca gacggaccuu gcaaggugcc cgcucaaaug     1620

gccgucgaua ugcagacccu gacaccugug ggcagacuca uuaccgcgaa ccccgugauc     1680

acggaaucca cagagaauag caagaugaug cuggagcuug aucccccuuu cggggacucu     1740

uacaucguua uuggcgucgg agagaaaaag aucacccacc acuggcaucg cuccggcagc     1800

acaauuggga aagccuuuga agcuaccgug cggggagcca agagaauggc ugugcugggc     1860

gacaccgccu gggauuucgg guccgucgga ggcgcccuca acucucuggg gaaaggcauc     1920

caccagauuu ucggagcugc guuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaua cgaagaacgg gagcauuucc     2040

cugauguguc uugcccuggg aggcgugcuc aucuuccuga gcaccgcugu uucugccgac     2100

guggggugcu ccgucgauuu uagcaaa                                         2127


<210>  2216
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2216
gccagaaagg agaaaaagcg gcgcggcgcu gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg ccauggcggc ugaaguuacc agacggggcu ccgccuacua uauguaccuu      120

gaucgcaacg acgccgggga ggcuauuucu uuccccacaa cccugggaau gaauaaaugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcca caaugagcua cgaaugcccu      240

augcuggaug agggggugga accggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaag aaaggcgagg cuagacgguc ucgcagagcc      360

guuacccuuc ccagccauuc cacacggaag cugcaaaccc gcucucagac auggcucgag      420

agcagagaau acaccaagca ccugauccgg guggagaauu ggauuuuucg caacccuggc      480

uucgcgcugg ccgcugccgc uaucgccugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcg cccgccuacu ccauuagaug caucggaguu      600

agcaaucggg auuuugugga aggcaugucu gggggaacau gggucgacau ugugcuggag      660

cauggcgggu gugugaccgu cauggcucag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggccgaaguc cgcagcuacu gcuaugaggc uucuauuucc      780

gauauggcca gcgacuccag augucccacg caaggagaag ccuaccugga caaacagucu      840

gauacccagu augugugcaa gcggacacuc guugacagag gcuggggcaa ugggugugga      900

cuguucggca aagggagccu ggugaccugc gcuaaguuug cgugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga gaaccucgaa uaccgcauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaga cggacgagaa ccgggccaaa     1080

guugaaauca cacccaauuc ucccagagcu gaggccaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugaaccucg caccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaagc acuggcucgu gcauaaagag ugguuccacg acauuccccu gccuuggcac     1260

gccggggcug auaccggaac accccauugg aacaauaagg aagcccuggu cgaguuuaaa     1320

gacgcgcacg cuaagcggca gacggugguc gugcucggcu cucaggaggg ggccguucau     1380

accgcccugg cuggcgcccu ugaagcugag auggauggag ccaagggcag acugagcucc     1440

gggcaccuca aaugccgccu gaagauggac aaacuucggc ugaagggagu gagcuauucu     1500

cucuguacag cggccuucac cuuuacaaaa aucccggcug aaacccugca uggcacgguc     1560

accguggagg uucaguacgc cgggacagac ggaccuugca aggugcccgc ucaaauggcc     1620

gucgauaugc agacccugac accugugggc agacucauua ccgcgaaccc cgugaucacg     1680

gaauccacag agaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcaucgcuc cggcagcaca     1800

auugggaaag ccuuugaagc uaccgugcgg ggagccaaga gaauggcugu gcugggcgac     1860

accgccuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa aggcauccac     1920

cagauuuucg gagcugcguu uaagagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cccugggagg cgugcucauc uuccugagca ccgcuguuuc ugccgacgug     2100

gggugcuccg ucgauuuuag caaa                                            2124


<210>  2217
<211>  2121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2217
agaaaggaga aaaagcggcg cggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga aguuaccaga cggggcuccg cuuacuauau guaccuugau      120

cgcaacgacg ccggggaggc cauuucuuuc cccacaaccc ugggaaugaa uaaaugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaagaaa ggcgaggcca gacggucucg cagagcuguu      360

acccuuccca gccauuccac acggaagcug caaacccgcu cucagacaug gcucgagagc      420

agagaauaca ccaagcaccu gauccgggug gagaauugga uuuuucgcaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uuagaugcau cggaguuagc      600

aaucgggauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaaguccgc agcuacugcu augaggccuc uauuuccgau      780

auggcuagcg acuccagaug ucccacgcaa ggagaagccu accuggacaa acagucugau      840

acccaguaug ugugcaagcg gacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaaag ggagccuggu gaccugcgcc aaguuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cgcaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgagaaccg ggcgaaaguu     1080

gaaaucacac ccaauucucc cagagccgag gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucgcac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaagcacu ggcucgugca uaaagagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaggaag cucuggucga guuuaaagac     1320

gcccacgcga agcggcagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga agccgagaug gauggagcua agggcagacu gagcuccggg     1440

caccucaaau gccgccugaa gauggacaaa cuucggcuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaaaauc ccggccgaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgcugg gacagacgga ccuugcaagg ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggcaga cucauuaccg ccaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgcuccgg cagcacaauu     1800

gggaaagcgu uugaagccac cgugcgggga gcuaagagaa uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaagg cauccaccag     1920

auuuucggag ccgcuuuuaa gagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacgugggg     2100

ugcuccgucg auuuuagcaa a                                               2121


<210>  2218
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2218
aaggagaaaa agagacgggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaagu uacccgcaga ggcuccgcuu acuauaugua ccuugaucgg      120

aacgacgccg gggaggccau uucuuucccc acaacccugg gaaugaauaa augcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgaaug cccuaugcug      240

gaugaggggg uggaaccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caagaaaggc gaggcccgca gaucucggcg cgcuguuacc      360

cuucccagcc auuccacaag aaagcugcaa acccggucuc agacauggcu cgagagccgc      420

gaauacacca agcaccugau cagaguggag aauuggauuu uucggaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauuc gcugcaucgg aguuagcaau      600

agagauuuug uggaaggcau gucuggggga acaugggucg acauugugcu ggagcauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga aguccggagc uacugcuaug aggccucuau uuccgauaug      780

gcuagcgacu cccgcugucc cacgcaagga gaagccuacc uggacaaaca gucugauacc      840

caguaugugu gcaagagaac acucguugac cggggcuggg gcaaugggug uggacuguuc      900

ggcaaaggga gccuggugac cugcgccaag uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugagaaccu cgaauacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gagacggacg agaaccgcgc gaaaguugaa     1080

aucacaccca auucuccccg ggccgaggcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugaac cuagaaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aagcacuggc ucgugcauaa agagugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaggaagcuc uggucgaguu uaaagacgcc     1320

cacgcgaagc gccagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaagc cgagauggau ggagcuaagg gccggcugag cuccgggcac     1440

cucaaaugca gacugaagau ggacaaacuu cgccugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaaaaucccg gccgaaaccc ugcauggcac ggucaccgug     1560

gagguucagu acgcugggac agacggaccu ugcaaggugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggccggcuc auuaccgcca accccgugau cacggaaucc     1680

acagagaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcaua gauccggcag cacaauuggg     1800

aaagcguuug aagccaccgu gcgcggagcu aagcggaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaaaggcau ccaccagauu     1920

uucggagccg cuuuuaagag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga cguggggugc     2100

uccgucgauu uuagcaaa                                                   2118


<210>  2219
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2219
gagaagaaaa gacggggcgc cgacaccagc guggggaucg ucggacugcu ccugacaacg       60

gcuauggccg cggaaguuac ccgcagaggc uccgcuuacu auauguaccu ugaucggaac      120

gacgccgggg aggccauuuc uuuccccaca acccugggaa ugaauaagug cuauauccag      180

auuauggauc ucggccacau gugugacgcu acaaugagcu acgaaugccc uaugcuggau      240

gagggggugg aaccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uauggaacgu gucaucacaa aaagggcgag gcccgcagau cucggcgcgc uguuacccuu      360

cccagccauu ccacaagaaa acugcaaacc cggucucaga cauggcucga gagccgcgaa      420

uacaccaagc accugaucag aguggagaau uggauuuuuc ggaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggg uccagcacgu cucagaaggu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauucgcu gcaucggagu uagcaauaga      600

gauuuugugg aaggcauguc ugggggaaca ugggucgaca uugugcugga gcauggcggg      660

ugugugaccg ucauggccca ggauaaaccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaagu ccggagcuac ugcuaugagg ccucuauuuc cgauauggcu      780

agcgacuccc gcugucccac gcaaggagaa gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aaagaacacu cguugaccgg ggcuggggca augggugugg acuguucggc      900

aaggggagcc uggugaccug cgccaaauuu gcuugcucca agaagaugac aggaaaaagc      960

auccagccug agaaccucga auacagaauc augcugucug uccacggcuc ccaacacagc     1020

gggaugauug ugaaugauac cggacaugag acggacgaga accgcgcgaa gguugaaauc     1080

acacccaauu cuccccgggc cgaggcuacc cuuggcggcu ucgggagccu gggacucgau     1140

ugugaaccua gaaccggccu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga gugguuccac gacauucccc ugccuuggca cgccggggcc     1260

gauaccggaa caccccauug gaacaauaaa gaagcucugg ucgaguuuaa ggacgcccac     1320

gcgaaacgcc agacgguggu cgugcucggc ucucaggagg gggcuguuca uaccgcccug     1380

gccggcgcuc uugaagccga gauggaugga gcuaagggcc ggcugagcuc cgggcaccuc     1440

aagugcagac ugaaaaugga caaacuucgc cugaagggag ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gaaacccugc auggcacggu caccguggag     1560

guucaguacg cugggacaga cggaccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg ccggcucauu accgccaacc ccgugaucac ggaauccaca     1680

gagaacagca agaugaugcu ggagcuugac ccgccuuucg gggauucuua caucguuauu     1740

ggcgucggag aaaagaaaau cacccaccac uggcauagau ccggcagcac aauugggaag     1800

gcguuugagg ccaccgugcg cggagcuaaa cggauggccg ugcugggcga caccgcuugg     1860

gauuucgggu ccgucggagg cgcccucaau ucucugggga agggcaucca ccagauuuuc     1920

ggagccgcuu uuaaaagccu guucggcggg auguccuggu uuucucaaau ccuuaucgga     1980

acacuccuga uguggcuggg ccucaacacg aagaauggga gcauuucccu gaugugucuu     2040

gcgcugggag gcgugcucau cuuccugagc accgccguuu cugcugacgu ggggugcucc     2100

gucgauuuua gcaag                                                      2115


<210>  2220
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2220
aagaaaagac ggggcgccga caccagcgug gggaucgucg gacugcuccu gacaacggcu       60

auggccgcgg agguuacccg cagaggcucc gcuuacuaua uguaccuuga ucggaacgac      120

gccggggaag ccauuucuuu ccccacaacc cugggaauga auaagugcua uauccagauu      180

auggaucucg gccacaugug ugacgcuaca augagcuacg agugcccuau gcuggaugaa      240

gggguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

ggaacguguc aucacaaaaa gggcgaagcc cgcagaucuc ggcgcgcugu uacccuuccc      360

agccauucca caagaaaacu gcaaacccgg ucucagacau ggcucgagag ccgcgaguac      420

accaagcacc ugaucagagu ggaaaauugg auuuuucgga acccuggcuu cgcccuggcg      480

gccgcugcca ucgcuuggcu ccuggggucc agcacgucuc agaaggucau cuaucuugug      540

augauucugc ucaucgcccc cgcguacucc auucgcugca ucggaguuag caauagagau      600

uuuguggagg gcaugucugg gggaacaugg gucgacauug ugcuggaaca uggcgggugu      660

gugaccguca uggcccagga uaaaccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg cugagguccg gagcuacugc uaugaagccu cuauuuccga uauggcuagc      780

gacucccgcu gucccacgca aggagaggcc uaccuggaca agcagucuga uacccaguau      840

gugugcaaaa gaacacucgu ugaccggggc uggggcaaug gguguggacu guucggcaag      900

gggagccugg ugaccugcgc caaauuugcu ugcuccaaga agaugacagg aaaaagcauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggcuccca acacagcggg     1020

augauuguga augauaccgg acaugaaacg gacgagaacc gcgcgaaggu ugagaucaca     1080

cccaauucuc cccgggccga agcuacccuu ggcggcuucg ggagccuggg acucgauugu     1140

gagccuagaa ccggccugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc cggggccgau     1260

accggaacac cccauuggaa caauaaagag gcucuggucg aauuuaagga cgcccacgcg     1320

aaacgccaga cgguggucgu gcucggcucu caggaggggg cuguucauac cgcccuggcc     1380

ggcgcucuug aggccgaaau ggauggagcu aagggccggc ugagcuccgg gcaccucaag     1440

ugcagacuga aaauggacaa acuucgccug aagggaguga gcuauucucu cuguacagcc     1500

gcguucaccu uuacaaagau cccggccgag acccugcaug gcacggucac cguggaaguu     1560

caguacgcug ggacagacgg accuugcaaa gugcccgccc aaauggcugu cgauaugcag     1620

acccugacac cugugggccg gcucauuacc gccaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucgggg auucuuacau cguuauuggc     1740

gucggagaga agaaaaucac ccaccacugg cauagauccg gcagcacaau ugggaaggcg     1800

uuugaagcca ccgugcgcgg agcuaaacgg auggccgugc ugggcgacac cgcuugggau     1860

uucggguccg ucggaggcgc ccucaauucu cuggggaagg gcauccacca gauuuucgga     1920

gccgcuuuua aaagccuguu cggcgggaug uccugguuuu cucaaauccu uaucggaaca     1980

cuccugaugu ggcugggccu caacacgaag aaugggagca uuucccugau gugucuugcg     2040

cugggaggcg ugcucaucuu ccugagcacc gccguuucug cugacguggg gugcuccguc     2100

gauuuuagca ag                                                         2112


<210>  2221
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2221
aagagacggg gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug       60

gccgcggagg uuacccgcag aggcuccgcu uacuauaugu accuugaucg gaacgacgcc      120

ggggaagcca uuucuuuccc cacaacccug ggaaugaaua aaugcuauau ccagauuaug      180

gaucucggcc acauguguga cgcuacaaug agcuacgagu gcccuaugcu ggaugaaggg      240

guggagccgg acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga      300

acgugucauc acaagaaagg cgaagcccgc agaucucggc gcgcuguuac ccuucccagc      360

cauuccacaa gaaagcugca aacccggucu cagacauggc ucgagagccg cgaguacacc      420

aaacaccuga ucagagugga aaauuggauu uuucggaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggguccagc acgucucaga aggucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu cgcugcaucg gaguuagcaa uagagauuuu      600

guggagggca ugucuggggg aacauggguc gacauugugc uggaacaugg cgggugugug      660

accgucaugg cccaggauaa gccuacaguu gacaucgagc uugugaccac cacagugucc      720

aacauggcug agguccggag cuacugcuau gaagccucua uuuccgauau ggcuagcgac      780

ucccgcuguc ccacgcaagg agaggccuac cuggacaaac agucugauac ccaguaugug      840

ugcaagagaa cacucguuga ccggggcugg ggcaaugggu guggacuguu cggcaaaggg      900

agccugguga ccugcgccaa guuugcuugc uccaaaaaga ugacaggaaa gagcauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gcucccaaca cagcgggaug     1020

auugugaaug auaccggaca ugaaacggac gagaaccgcg cgaaaguuga gaucacaccc     1080

aauucucccc gggccgaagc uacccuuggc ggcuucggga gccugggacu cgauugugag     1140

ccuagaaccg gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaagcacugg     1200

cucgugcaua aagaaugguu ccacgacauu ccccugccuu ggcacgccgg ggccgauacc     1260

ggaacacccc auuggaacaa uaaggaggcu cuggucgaau uuaaagacgc ccacgcgaag     1320

cgccagacgg uggucgugcu cggcucucag gagggggcug uucauaccgc ccuggccggc     1380

gcucuugagg ccgaaaugga uggagcuaaa ggccggcuga gcuccgggca ccucaagugc     1440

agacugaaga uggacaaacu ucgccugaag ggagugagcu auucucucug uacagccgcg     1500

uucaccuuua caaaaauccc ggccgagacc cugcauggca cggucaccgu ggaaguucag     1560

uacgcuggga cagacggacc uugcaaggug cccgcccaaa uggcugucga uaugcagacc     1620

cugacaccug ugggccggcu cauuaccgcc aaccccguga ucacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugacccgccu uucggggauu cuuacaucgu uauuggcguc     1740

ggagagaaga agaucaccca ccacuggcau agauccggca gcacaauugg gaaagcguuu     1800

gaagccaccg ugcgcggagc uaagcggaug gccgugcugg gcgacaccgc uugggauuuc     1860

ggguccgucg gaggcgcccu caauucucug gggaaaggca uccaccagau uuucggagcc     1920

gcuuuuaaga gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc     1980

cugauguggc ugggccucaa cacgaaaaau gggagcauuu cccugaugug ucuugcgcug     2040

ggaggcgugc ucaucuuccu gagcaccgcc guuucugcug acguggggug cuccgucgau     2100

uuuagcaag                                                             2109


<210>  2222
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2222
agacggggcg ccgacaccag cguggggauc gucggacugc uccugacaac ggcuauggcc       60

gcggagguua cccgcagagg cuccgcuuac uauauguacc uugaucggaa cgacgccggg      120

gaagccauuu cuuuccccac aacccuggga augaauaagu gcuauaucca gauuauggau      180

cucggccaca ugugugacgc uacaaugagc uacgagugcc cuaugcugga ugaaggggug      240

gagccggacg auguggacug uuggugcaac accacaucca ccugggucgu guauggaacg      300

ugucaucaca aaaagggcga agcccgcaga ucucggcgcg cuguuacccu ucccagccau      360

uccacaagaa aacugcaaac ccggucucag acauggcucg agagccgcga guacaccaag      420

caccugauca gaguggaaaa uuggauuuuu cggaacccug gcuucgcccu ggcggccgcu      480

gccaucgcuu ggcuccuggg guccagcacg ucucagaaag ucaucuaucu ugugaugauu      540

cugcucaucg cccccgcgua cuccauucgc ugcaucggag uuagcaauag agauuuugug      600

gagggcaugu cugggggaac augggucgac auugugcugg aacauggcgg gugugugacc      660

gucauggccc aggauaagcc uacaguugac aucgagcuug ugaccaccac aguguccaac      720

auggcugagg uccggagcua cugcuaugaa gccucuauuu ccgauauggc uagcgacucc      780

cgcuguccca cgcaaggaga ggccuaccug gacaagcagu cugauaccca guaugugugc      840

aaaagaacac ucguugaccg gggcuggggc aaugggugug gacuguucgg caaggggagc      900

cuggugaccu gcgccaaauu ugcuugcucc aagaaaauga caggaaagag cauccagccu      960

gaaaaccucg aguacagaau caugcugucu guccacggcu cccaacacag cgggaugauu     1020

gugaaugaua ccggacauga aacggacgag aaccgcgcga agguugagau cacacccaau     1080

ucuccccggg ccgaagcuac ccuuggcggc uucgggagcc ugggacucga uugugagccu     1140

agaaccggcc uggacuuuuc cgaucuuuau uaccugacaa ugaacaauaa acacuggcuc     1200

gugcauaagg aaugguucca cgacauuccc cugccuuggc acgccggggc cgauaccgga     1260

acaccccauu ggaacaauaa agaggcucug gucgaauuua aggacgccca cgcgaaacgc     1320

cagacggugg ucgugcucgg cucucaggag ggggcuguuc auaccgcccu ggccggcgcu     1380

cuugaggccg aaauggaugg agcuaagggc cggcugagcu ccgggcaccu caaaugcaga     1440

cugaagaugg acaaacuucg ccugaaggga gugagcuauu cucucuguac agccgcguuc     1500

accuuuacaa agaucccggc cgagacccug cauggcacgg ucaccgugga aguucaguac     1560

gcugggacag acggaccuug caaagugccc gcccaaaugg cugucgauau gcagacccug     1620

acaccugugg gccggcucau uaccgccaac cccgugauca cggaguccac agaaaacagc     1680

aagaugaugc uggagcuuga cccgccuuuc ggggauucuu acaucguuau uggcgucgga     1740

gagaaaaaga ucacccacca cuggcauaga uccggcagca caauugggaa ggcguuugaa     1800

gccaccgugc gcggagcuaa acggauggcc gugcugggcg acaccgcuug ggauuucggg     1860

uccgucggag gcgcccucaa uucucugggg aagggcaucc accagauuuu cggagccgcu     1920

uuuaaaagcc uguucggcgg gauguccugg uuuucucaaa uccuuaucgg aacacuccug     1980

auguggcugg gccucaacac gaagaauggg agcauuuccc ugaugugucu ugcgcuggga     2040

ggcgugcuca ucuuccugag caccgccguu ucugcugacg uggggugcuc cgucgauuuu     2100

agcaaa                                                                2106


<210>  2223
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2223
agaggcgccg acaccagcgu ggggaucguc ggacugcucc ugacaacggc uauggccgcg       60

gagguuaccc ggcgcggcuc cgcuuacuau auguaccuug auagaaacga cgccggggaa      120

gccauuucuu uccccacaac ccugggaaug aauaagugcu auauccagau uauggaucuc      180

ggccacaugu gugacgcuac aaugagcuac gagugcccua ugcuggauga agggguggag      240

ccggacgaug uggacuguug gugcaacacc acauccaccu gggucgugua uggaacgugu      300

caucacaaaa agggcgaagc ccggcgcucu agacgggcug uuacccuucc cagccauucc      360

acacgcaaac ugcaaaccag aucucagaca uggcucgaga gccgggagua caccaagcac      420

cugauccgcg uggaaaauug gauuuuuaga aacccuggcu ucgcccuggc ggccgcugcc      480

aucgcuuggc uccugggguc cagcacgucu cagaaaguca ucuaucuugu gaugauucug      540

cucaucgccc ccgcguacuc cauucggugc aucggaguua gcaaucgcga uuuuguggag      600

ggcaugucug ggggaacaug ggucgacauu gugcuggaac auggcgggug ugugaccguc      660

auggcccagg auaagccuac aguugacauc gagcuuguga ccaccacagu guccaacaug      720

gcugagguca gaagcuacug cuaugaagcc ucuauuuccg auauggcuag cgacucccgg      780

ugucccacgc aaggagaggc cuaccuggac aagcagucug auacccagua ugugugcaaa      840

cgcacacucg uugacagagg cuggggcaau ggguguggac uguucggcaa ggggagccug      900

gugaccugcg ccaaauuugc uugcuccaag aaaaugacag gaaagagcau ccagccugaa      960

aaccucgagu accggaucau gcugucuguc cacggcuccc aacacagcgg gaugauugug     1020

aaugauaccg gacaugaaac ggacgagaac agagcgaagg uugagaucac acccaauucu     1080

ccccgcgccg aagcuacccu uggcggcuuc gggagccugg gacucgauug ugagccucgg     1140

accggccugg acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug     1200

cauaaggaau gguuccacga cauuccccug ccuuggcacg ccggggccga uaccggaaca     1260

ccccauugga acaauaaaga ggcucugguc gaauuuaagg acgcccacgc gaaaagacag     1320

acgguggucg ugcucggcuc ucaggagggg gcuguucaua ccgcccuggc cggcgcucuu     1380

gaggccgaaa uggauggagc uaagggccgc cugagcuccg ggcaccucaa augccggcug     1440

aagauggaca aacuuagacu gaagggagug agcuauucuc ucuguacagc cgcguucacc     1500

uuuacaaaga ucccggccga gacccugcau ggcacgguca ccguggaagu ucaguacgcu     1560

gggacagacg gaccuugcaa agugcccgcc caaauggcug ucgauaugca gacccugaca     1620

ccugugggcc gccucauuac cgccaacccc gugaucacgg aguccacaga aaacagcaag     1680

augaugcugg agcuugaccc gccuuucggg gauucuuaca ucguuauugg cgucggagag     1740

aaaaagauca cccaccacug gcaucggucc ggcagcacaa uugggaaggc guuugaagcc     1800

accgugagag gagcuaaacg cauggccgug cugggcgaca ccgcuuggga uuucgggucc     1860

gucggaggcg cccucaauuc ucuggggaag ggcauccacc agauuuucgg agccgcuuuu     1920

aaaagccugu ucggcgggau guccugguuu ucucaaaucc uuaucggaac acuccugaug     1980

uggcugggcc ucaacacgaa gaaugggagc auuucccuga ugugucuugc gcugggaggc     2040

gugcucaucu uccugagcac cgccguuucu gcugacgugg ggugcuccgu cgauuuuagc     2100

aaa                                                                   2103


<210>  2224
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2224
ggcgccgaca ccagcguggg gaucgucgga cugcuccuga caacggcuau ggccgcggag       60

guuaccagac ggggcuccgc uuacuauaug uaccuugauc gcaacgacgc cggggaagcc      120

auuucuuucc ccacaacccu gggaaugaau aagugcuaua uccagauuau ggaucucggc      180

cacaugugug acgcuacaau gagcuacgag ugcccuaugc uggaugaagg gguggagccg      240

gacgaugugg acuguuggug caacaccaca uccaccuggg ucguguaugg aacgugucau      300

cacaaaaagg gcgaagccag acggucucgc agagcuguua cccuucccag ccauuccaca      360

cggaaacugc aaacccgcuc ucagacaugg cucgagagca gagaguacac caagcaccug      420

auccgggugg aaaauuggau uuuucgcaac ccuggcuucg cccuggcggc cgcugccauc      480

gcuuggcucc ugggguccag cacgucucag aaagucaucu aucuugugau gauucugcuc      540

aucgcccccg cguacuccau uagaugcauc ggaguuagca aucgggauuu uguggagggc      600

augucugggg gaacaugggu cgacauugug cuggaacaug gcgggugugu gaccgucaug      660

gcccaggaua agccuacagu ugacaucgag cuugugacca ccacaguguc caacauggcu      720

gagguccgca gcuacugcua ugaagccucu auuuccgaua uggcuagcga cuccagaugu      780

cccacgcaag gagaggccua ccuggacaag cagucugaua cccaguaugu gugcaaacgg      840

acacucguug accgcggcug gggcaauggg uguggacugu ucggcaaggg gagccuggug      900

accugcgcca aauuugcuug cuccaagaaa augacaggaa agagcaucca gccugaaaac      960

cucgaguaca gaaucaugcu gucuguccac ggcucccaac acagcgggau gauugugaau     1020

gauaccggac augaaacgga cgagaaccgg gcgaagguug agaucacacc caauucuccc     1080

agagccgaag cuacccuugg cggcuucggg agccugggac ucgauuguga gccucgcacc     1140

ggccuggacu uuuccgaucu uuauuaccug acaaugaaca auaaacacug gcucgugcau     1200

aaggaauggu uccacgacau uccccugccu uggcacgccg gggccgauac cggaacaccc     1260

cauuggaaca auaaagaggc ucuggucgaa uuuaaggacg cccacgcgaa acggcagacg     1320

guggucgugc ucggcucuca ggagggggcu guucauaccg cccuggccgg cgcucuugag     1380

gccgaaaugg auggagcuaa gggcagacug agcuccgggc accucaaaug ccgccugaag     1440

auggacaaac uucggcugaa gggagugagc uauucucucu guacagccgc guucaccuuu     1500

acaaagaucc cggccgagac ccugcauggc acggucaccg uggaaguuca guacgcuggg     1560

acagacggac cuugcaaagu gcccgcccaa auggcugucg auaugcagac ccugacaccu     1620

gugggcagac ucauuaccgc caaccccgug aucacggagu ccacagaaaa cagcaagaug     1680

augcuggagc uugacccgcc uuucggggau ucuuacaucg uuauuggcgu cggagagaaa     1740

aagaucaccc accacuggca ucgcuccggc agcacaauug ggaaggcguu ugaagccacc     1800

gugcggggag cuaaaagaau ggccgugcug ggcgacaccg cuugggauuu cggguccguc     1860

ggaggcgccc ucaauucucu ggggaagggc auccaccaga uuuucggagc cgcuuuuaaa     1920

agccuguucg gcgggauguc cugguuuucu caaauccuua ucggaacacu ccugaugugg     1980

cugggccuca acacgaagaa ugggagcauu ucccugaugu gucuugcgcu gggaggcgug     2040

cucaucuucc ugagcaccgc cguuucugcu gacguggggu gcuccgucga uuuuagcaaa     2100


<210>  2225
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2225
gccgacacca gcgugggcau cgucgggcug cuccugacaa cggcuauggc cgcggagguu       60

accagacggg gauccgcuua cuauauguac cuugaucgca acgacgccgg cgaagccauu      120

ucuuucccca caacccuggg gaugaauaag ugcuauaucc agauuaugga ucucggacac      180

augugugacg cuacaaugag cuacgagugc ccuaugcugg augaaggcgu ggagccggac      240

gauguggacu guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac      300

aaaaagggag aagccagacg gucucgcaga gcuguuaccc uucccagcca uuccacacgg      360

aaacugcaaa cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc      420

cggguggaaa auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu      480

uggcuccugg gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc      540

gcccccgcgu acuccauuag augcaucggg guuagcaauc gggauuuugu ggagggaaug      600

ucuggcggga caugggucga cauugugcug gaacauggag gcugugugac cgucauggcc      660

caggauaagc cuacaguuga caucgagcuu gugaccacca caguguccaa cauggcugag      720

guccgcagcu acugcuauga agccucuauu uccgauaugg cuagcgacuc cagauguccc      780

acgcaagggg aggccuaccu ggacaagcag ucugauaccc aguaugugug caaacggaca      840

cucguugacc gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc      900

ugcgccaaau uugcuugcuc caagaaaaug acaggaaaga gcauccagcc ugaaaaccuc      960

gaguacagaa ucaugcuguc uguccacggg ucccaacaca gcggcaugau ugugaaugau     1020

accgggcaug aaacggacga gaaccgggcg aagguugaga ucacacccaa uucucccaga     1080

gccgaagcua cccuuggagg cuucggcagc cuggggcucg auugugagcc ucgcaccgga     1140

cuggacuuuu ccgaucuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag     1200

gaaugguucc acgacauucc ccugccuugg cacgccggcg ccgauaccgg gacaccccau     1260

uggaacaaua aagaggcucu ggucgaauuu aaggacgccc acgcgaaacg gcagacggug     1320

gucgugcucg gaucucagga gggcgcuguu cauaccgccc uggccggggc ucuugaggcc     1380

gaaauggaug gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug     1440

gacaagcugc ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca     1500

aagaucccgg ccgagacccu gcauggaacg gucaccgugg aaguucagua cgcuggcaca     1560

gacgggccuu gcaaagugcc cgcccaaaug gcugucgaua ugcagacccu gacaccugug     1620

ggaagacuca uuaccgccaa ccccgugauc acggagucca cagaaaacag caagaugaug     1680

cuggagcuug acccgccuuu cggcgauucu uacaucguua uuggggucgg cgagaaaaag     1740

aucacccacc acuggcaucg cuccggaagc acaauuggca aggcguuuga agccaccgug     1800

agaggggcua aacggauggc cgugcuggga gacaccgcuu gggauuucgg cuccgucggg     1860

ggagcccuca auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc     1920

cuguucggag gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug     1980

ggacucaaca cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc     2040

aucuuccuga gcaccgccgu uucugcugac gugggcugcu ccgucgauuu uagcaaa        2097


<210>  2226
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2226
gacaccagcg ugggcaucgu cgggcugcuc cugacaacgg ccauggcugc cgagguuacc       60

agacggggau ccgcguacua uauguaccuu gaucgcaacg acgcuggcga agccauuucu      120

uuccccacaa cccuggggau gaauaagugc uauauccaga uuauggaucu cggacacaug      180

ugugacgcca caaugagcua cgagugcccu augcuggaug aaggcgugga gccggacgau      240

guggacuguu ggugcaacac cacauccacc ugggucgugu augggacgug ucaucacaaa      300

aagggagaag cuagacgguc ucgcagagcc guuacccuuc ccagccauuc cacacggaaa      360

cugcaaaccc gcucucagac auggcucgag agcagagagu acaccaagca ccugauccgg      420

guggaaaauu ggauuuuucg caacccuggc uucgcucugg ccgcggccgc uaucgccugg      480

cuccugggcu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcu      540

cccgccuacu ccauuagaug caucgggguu agcaaucggg auuuugugga gggaaugucu      600

ggcgggacau gggucgacau ugugcuggaa cauggaggcu gugugaccgu cauggcgcag      660

gauaagccua caguugacau cgagcuugug accaccacag uguccaacau ggccgagguc      720

cgcagcuacu gcuaugaagc uagcauuucc gauauggcca gcgacuccag augucccacg      780

caaggggagg cuuaccugga caagcagucu gauacccagu augugugcaa acggacacuc      840

guugaccgcg gauggggcaa uggcuguggg cuguucggaa agggcagccu ggugaccugc      900

gccaaauuug ccugcuccaa gaaaaugaca gggaagucua uccagccuga aaaccucgag      960

uacagaauca ugcugucugu ccacggaucc caacacagcg gcaugauugu gaaugauacc     1020

gggcaugaaa cggacgagaa ccgggcuaag guugagauca cacccaauuc ucccagagcg     1080

gaagccaccc uuggaggcuu cggcagccug gggcucgauu gugagccucg caccggacug     1140

gacuuuuccg aucuuuauua ccugacaaug aacaauaaac acuggcucgu gcauaaggaa     1200

ugguuccacg acauuccccu gccuuggcac gcuggcgccg auaccgggac accccauugg     1260

aacaauaaag aggcccuggu cgaauuuaag gacgcucacg ccaaacggca gacggugguc     1320

gugcucggau cucaggaggg cgcgguucau accgcucugg ccggggcccu ugaggcugaa     1380

auggauggcg ccaagggaag acugagcucc ggccaccuca aaugccgccu gaagauggac     1440

aagcugcggc ugaaaggggu gagcuauucu cucuguacag cugccuucac cuuuacaaag     1500

aucccggcgg agacccugca uggaacgguc accguggaag uucaguacgc cggcacagac     1560

gggccuugca aagugcccgc ucaaauggcc gucgauaugc agacccugac accuguggga     1620

agacucauua ccgcuaaccc cgugaucacg gaguccacag aaaacagcaa gaugaugcug     1680

gagcuugacc cgccuuucgg cgauucuuac aucguuauug gggucggcga gaaaaagauc     1740

acccaccacu ggcaucgcuc cggaagcaca auuggcaagg ccuuugaagc gaccgugaga     1800

ggggccaaac ggauggcugu gcugggagac accgccuggg auuucggcuc cgucggggga     1860

gcucucaauu cucugggcaa ggggauccac cagauuuucg gcgccgccuu uaaaagccug     1920

uucggaggca uguccugguu uucucaaauc cuuaucggga cacuccugau guggcuggga     1980

cucaacacga agaauggcag cauuucccug augugucuug cucuuggggg agugcucauc     2040

uuccugagca ccgcgguuuc ugccgacgug ggcugcuccg ucgauuuuag caaa           2094


<210>  2227
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2227
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc cgaugugggc ugcuccgucg acuuuagcaa a              2091


<210>  2228
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2228
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccgc gguuaccaga       60

cggggauccg cuuacuauau guaccuugac cgcaacgaug ccggcgaggc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugcuacaa ugagcuacga augcccuaug cuggacgagg gcguggaacc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaggcca gacggucucg cagagcuguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgaaagc agagaguaca ccaagcaccu gauccgggug      420

gagaauugga uuuuucgcaa cccuggcuuc gcccuggcgg ccgcugccau cgcuuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgccccc      540

gcguacucca uuagaugcau cgggguuagc aaucgggacu uuguggaagg aaugucuggc      600

gggacauggg ucgauauugu gcuggagcau ggaggcugug ugaccgucau ggcccaggac      660

aagccuacag uugauaucga acuugugacc accacagugu ccaacauggc ugagguccgc      720

agcuacugcu augaggccuc uauuuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaagccu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugcuu gcuccaagaa aaugacaggg aagucuaucc agccugagaa ccucgaauac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugagacgg augaaaaccg ggcgaagguu gagaucacac cgaauagccc cagagccgag     1080

gcuacccuug gaggcuucgg cagccugggg cucgacugug aaccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggagugg     1200

uuccacgaua uuccccugcc uuggcacgcc ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagaag cucuggucga guuuaaggau gcccacgcga aacggcagac gguggucgug     1320

cucggaucuc aggaaggcgc uguucauacc gcccuggccg gggcucuuga ggccgagaug     1380

gacggcgcua agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagccg cguucaccuu uacaaagauc     1500

ccggccgaaa cccugcaugg aacggucacc guggagguuc aguacgcugg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcuguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg ccaaccccgu gaucacggaa uccacagaga acagcaagau gaugcuggaa     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggcgu uugaggccac cgugagaggg     1800

gcuaaacgga uggccgugcu gggagauacc gcuugggacu ucggcuccgu cgggggagcc     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcgc uugggggagu gcucaucuuc     2040

cugagcaccg ccguuucugc ugaugugggc ugcuccgucg acuuuagcaa a              2091


<210>  2229
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2229
agaggcgccg acaccagcgu ggggaucguc ggacugcucc ugacaacggc uauggccgcg       60

gcuguuaccc ggcgcggcuc cgccuacuau auguaccuug auagaaacga cgccggggag      120

gcuauuucuu uccccacaac ccugggaaug aauaagugcu auauccagau uauggaucuc      180

ggccacaugu gugacgccac aaugagcuac gaaugcccua ugcuggauga ggggguggaa      240

ccggacgaug uggacuguug gugcaacacc acauccaccu gggucgugua uggaacgugu      300

caucacaaaa agggcgaggc ucggcgcucu agacgggccg uuacccuucc cagccauucc      360

acacgcaaac ugcaaaccag aucucagaca uggcucgaaa gccgggagua caccaagcac      420

cugauccgcg uggagaauug gauuuuuaga aacccuggcu ucgcgcuggc cgcugccgcu      480

aucgccuggc uccugggguc cagcacgucu cagaaaguca ucuaucuugu gaugauucug      540

cucaucgcgc ccgccuacuc cauucggugc aucggaguua gcaaucgcga uuuuguggaa      600

ggcaugucug ggggaacaug ggucgacauu gugcuggagc auggcgggug ugugaccguc      660

auggcucagg auaagccuac aguugacauc gaacuuguga ccaccacagu guccaacaug      720

gccgagguca gaagcuacug cuaugaggcu ucuauuuccg auauggccag cgacucccgg      780

ugucccacgc aaggagaagc cuaccuggac aagcagucug auacccagua ugugugcaaa      840

cgcacacucg uugacagagg cuggggcaau ggguguggac uguucggcaa ggggagccug      900

gugaccugcg cuaaauuugc gugcuccaag aaaaugacag gaaagagcau ccagccugag      960

aaccucgaau accggaucau gcugucuguc cacggcuccc aacacagcgg gaugauugug     1020

aaugauaccg gacaugagac ggacgaaaac agagccaagg uugagaucac acccaauucu     1080

ccccgcgcug aggccacccu uggcggcuuc gggagccugg gacucgauug ugaaccucgg     1140

accggccugg acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug     1200

cauaaggagu gguuccacga cauuccccug ccuuggcacg ccggggcuga uaccggaaca     1260

ccccauugga acaauaaaga agcccugguc gaguuuaagg acgcgcacgc uaaaagacag     1320

acgguggucg ugcucggcuc ucaggaaggg gccguucaua ccgcccuggc uggcgcccuu     1380

gaggcugaga uggauggagc caagggccgc cugagcuccg ggcaccucaa augccggcug     1440

aagauggaca aacuuagacu gaagggagug agcuauucuc ucuguacagc ggccuucacc     1500

uuuacaaaga ucccggcuga aacccugcau ggcacgguca ccguggaggu ucaguacgcc     1560

gggacagacg gaccuugcaa agugcccgcu caaauggccg ucgauaugca gacccugaca     1620

ccugugggcc gccucauuac cgcgaacccc gugaucacgg aauccacaga gaacagcaag     1680

augaugcugg aacuugaccc gccuuucggg gauucuuaca ucguuauugg cgucggagag     1740

aaaaagauca cccaccacug gcaucggucc ggcagcacaa uugggaaggc cuuugaggcu     1800

accgugagag gagccaaacg cauggcugug cugggcgaca ccgccuggga uuucgggucc     1860

gucggaggcg cccucaauuc ucuggggaag ggcauccacc agauuuucgg agcugcguuu     1920

aaaagccugu ucggcgggau guccugguuu ucucaaaucc uuaucggaac acuccugaug     1980

uggcugggcc ucaacacgaa gaaugggagc auuucccuga ugugucuugc ccugggaggc     2040

gugcucaucu uccugagcac cgcuguuucu gccgacgugg ggugcuccgu cgauuuuagc     2100

aaa                                                                   2103


<210>  2230
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2230
agaggccuga gcucccggaa gcgcagaucu cacgacgugc ucaccgucca guuccugauc       60

cuugggaugc ugcucaugac aggaggcguu acgcggcgcg ggagcgccua cuauauguac      120

cuggauagaa acgacgcugg agaggccauu uccuuuccca ccacacuugg caugaauaaa      180

ugcuauaucc aaauuaugga ucuggggcau augugugacg cgaccauguc uuacgaaugc      240

ccuaugcucg augagggagu ggaaccggac gauguggacu guuggugcaa cacaaccagc      300

acaugggucg uguauggcac cugucaccau aagaaaggcg aggcucggcg cuccagacgg      360

gccguuacgc ugcccucuca cagcacccgc aagcugcaga caagauccca gaccuggcuc      420

gaaagccggg aguacacaaa acaucugauc cgcguggaga auuggauuuu cagaaacccu      480

ggguuugccc uugcugccgc ugccaucgcg uggcugcucg gaucuuccac cagccagaag      540

gucaucuauc uggugaugau ucuucugauc gcccccgcuu acucuauucg gugcaucggc      600

guuuccaauc gcgauuucgu ggaagggaug agcggaggca cgugggucga cauugugcuc      660

gagcacgggg gaugugugac agucauggcc caagauaagc cuaccguuga caucgaacug      720

gugacaacca ccgugucuaa cauggcugag gucagauccu acugcuauga ggccagcauu      780

uccgauaugg cgucugacag ccgguguccc acacagggcg aagccuaccu ggacaaacag      840

uccgauacgc aguaugugug caagagaacc cucguugacc gcggcugggg gaauggaugu      900

ggccuguuug ggaaaggaag ccuugugaca ugcgcuaagu ucgccugcuc uaaaaagaug      960

accggcaagu ccauccaacc ugagaaccug gaauaccgga ucaugcucag cguccacggg     1020

ucucagcaua gcggaaugau ugugaaugau acaggccacg agaccgacga aaacagagcu     1080

aaaguugaga ucacgcccaa uuccccgcgc gccgaggcca cacugggcgg guuuggaucu     1140

cuuggccugg auugugaacc ucggaccggg cucgacuuca gcgaucugua uuaccugacc     1200

augaacaaua agcauuggcu cgugcacaaa gagugguuuc acgacauucc ccugccuugg     1260

caugcuggag cggauacagg cacaccccac uggaacaaua aggaagcccu ugucgaguuc     1320

aaagacgcuc augccaagag acagacagug gucgugcugg ggucccagga aggcgccguu     1380

cacacggcuc ucgccggagc gcuggaggcu gagauggaug gcgccaaagg gcgccuuagc     1440

ucuggacauc ugaagugccg gcucaagaug gacaaacuga gacugaaggg cguguccuau     1500

agccucugua ccgccgcuuu uacauucacc aaaaucccgg ccgaaacacu gcacgggacc     1560

gucacggugg agguucaaua cgcuggaacc gacggcccuu gcaaggugcc cgcccagaug     1620

gcggucgaua ugcagacacu uaccccugug gggcgccuga uuacagccaa ccccgugauc     1680

accgaaucua cggagaacuc caaaaugaug cucgaacugg aucccccuuu uggcgacagc     1740

uacaucguua uuggagucgg cgagaagaag aucacacacc auuggcaccg guccgggucu     1800

accauuggaa aggcuuucga ggccacagug agaggcgcua aacgcauggc cgugcugggg     1860

gacaccgcgu gggauuucgg aagcgucggc ggggcccuua auucccucgg caaaggaauc     1920

caucaaauuu uuggcgcugc cuucaagucu cuguuugggg gaaugagcug guucucccag     1980

auccugaucg gcacccuccu gauguggcuu gggcugaaca ccaaaaaugg aagcauuucu     2040

cucauguguc uggcucuggg cggggugcuu aucuuucucu ccacggccgu uagcgccgac     2100

gugggcugcu cugucgauuu cuccaag                                         2127


<210>  2231
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2231
agaggccuga gcucccggaa gcgcagaucu cacgacgugc ucaccgucca guuccugauc       60

cuugggaugc ugcucaugac aggagccguu acgcggcgcg gcagcgcuua cuauauguac      120

cuggauagaa acgacgccgg ggaggcgauu uccuuuccca ccacacuugg aaugaauaaa      180

ugcuauaucc aaauuaugga ucugggccau augugugacg cuaccauguc uuacgaaugc      240

ccuaugcucg augagggggu ggaaccggac gauguggacu guuggugcaa cacaaccagc      300

acaugggucg uguauggaac cugucaccau aagaaaggcg aggcccggcg cuccagacgg      360

gccguuacgc ugcccucuca cagcacccgc aagcugcaga caagauccca gaccuggcuc      420

gaaagccggg aguacacaaa acaucugauc cgcguggaga auuggauuuu cagaaacccu      480

ggcuuugcuc uugccgcugc cgcgaucgcc uggcugcucg ggucuuccac cagccagaag      540

gucaucuauc uggugaugau ucuucugauc gcucccgccu acucuauucg gugcaucgga      600

guuuccaauc gcgauuucgu ggaaggcaug agcgggggaa cgugggucga cauugugcuc      660

gagcacggcg ggugugugac agucauggcu caagauaagc cuaccguuga caucgaacug      720

gugacaacca ccgugucuaa cauggccgag gucagauccu acugcuauga ggcgagcauu      780

uccgauaugg ccucugacag ccgguguccc acacagggag aggcuuaccu ggacaaacag      840

uccgauacgc aguaugugug caagagaacc cucguugacc gcggcugggg caaugggugu      900

ggacuguuug gcaaagggag ccuugugaca ugcgccaagu ucgcuugcuc uaaaaagaug      960

accggaaagu ccauccaacc ugaaaaccug gaauaccgga ucaugcucag cguccacggc     1020

ucucagcaua gcgggaugau ugugaaugau acaggacacg agaccgacga aaacagagcc     1080

aaaguugaga ucacgcccaa uuccccgcgc gccgaggcua cacugggcgg cuuugggucu     1140

cuuggacugg auugugaacc ucggaccggc cucgacuuca gcgaucugua uuaccugacc     1200

augaacaaua agcauuggcu cgugcacaaa gagugguuuc acgacauucc ccugccuugg     1260

caugcggggg ccgauacagg aacaccccac uggaacaaua aggaagcucu ugucgaguuc     1320

aaagacgccc augccaagag acagacagug gucgugcugg gcucccagga aggggcuguu     1380

cacacggccc ucgcgggcgc ucuggaggcc gagauggaug gagccaaagg ccgccuuagc     1440

ucugggcauc ugaagugccg gcucaagaug gacaaacuga gacugaaggg aguguccuau     1500

agccucugua ccgcugccuu uacauucacc aaaaucccgg cugaaacacu gcacggcacc     1560

gucacggugg agguucaaua cgccgggacc gacggaccuu gcaaggugcc cgcgcagaug     1620

gccgucgaua ugcagacacu uaccccugug ggccgccuga uuacagcuaa ccccgugauc     1680

accgaaucua cggagaacuc caaaaugaug cucgaacugg aucccccuuu uggggacagc     1740

uacaucguua uuggcgucgg agagaagaag aucacacacc auuggcaccg guccggcucu     1800

accauuggga aagccuucga ggcuacagug agaggagcca agcgcauggc ggugcugggc     1860

gacaccgccu gggauuucgg gagcgucgga ggcgcucuua auucccucgg gaaaggcauc     1920

caucaaauuu uuggagccgc uuucaagucu cuguuuggcg ggaugagcug guucucccag     1980

auccugaucg gaacccuccu gauguggcuu ggccugaaca ccaaaaaugg gagcauuucu     2040

cucauguguc uggcccuggg aggcgugcuu aucuuucucu ccacggccgu uagcgcugac     2100

guggggugcu cugucgauuu cuccaag                                         2127


<210>  2232
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2232
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaau auggccgaag ucagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguugaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcgca cgcuaagaga cagacggugg ucgugcucgg cucucaggag     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccgugauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcgugcuca ucuuccugag caccgcuguu     2100

ucugccgacg uggggugc                                                   2118


<210>  2233
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2233
aucauuaacg ccagaaagga gaaaaagcgg cgcggcgcug acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc cauggcggcu gaaguuacca gacggggcuc cgccuacuau      120

auguaccuug aucgcaauga cgccggggag gcuauuucuu uccccacaac ccugggaaug      180

aacaaaugcu auauccagau uauggaucuc ggccacaugu gugacgccac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaauacc      300

acauccaccu gggucgugua uggaacgugu caucacaaga aaggcgaggc uagacggucu      360

cgcagagccg uuacccuucc cagccauucc acacggaagc ugcaaacccg cucucagaca      420

uggcucgaga gcagagaaua caccaagcac cugauccggg uggagaacug gaucuuucgc      480

aauccuggcu ucgcgcuggc cgcugccgcu auugccuggc uccugggguc cagcacgucu      540

cagaaaguca ucuaucuugu gaugauucug cucaucgcgc ccgccuacuc cauuagaugc      600

aucggaguua gcaaccggga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcucagg auaagccuac aguugacauc      720

gagcuuguga ccaccacagu guccaauaug gccgaagucc gcagcuacug cuaugaggcu      780

ucuaucuccg auauggccag cgacuccaga ugucccacgc aaggagaagc cuaccuggac      840

aaacagucug auacccagua ugugugcaag cggacacucg uugacagagg cuggggcaac      900

ggguguggac uguucggcaa agggagccug gugaccugcg cuaaguuugc gugcuccaag      960

aaaaugacag gaaagagcau ucagccugag aaucucgaau accgcaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aacgauaccg gacaugagac ggacgagaau     1080

cgggccaaag uugaaaucac accgaacucu cccagagcug aggccacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccucgc accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga auaacaagca cuggcucgug cauaaagagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggcuga uaccggaaca ccccauugga auaacaagga agcccugguc     1320

gaguuuaaag acgcgcacgc uaagcggcag acgguggucg ugcucggcuc ucaggagggg     1380

gccguucaua ccgcccuggc uggcgcccuu gaagcugaga uggauggagc caagggcaga     1440

cugagcuccg ggcaccucaa augccgccug aagauggaca aacuucggcu gaagggagug     1500

agcuauucuc ucuguacagc ggccuucacc uuuacaaaaa ucccggcuga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcc gggacagacg gaccuugcaa ggugcccgcu     1620

caaauggccg ucgauaugca gacccugaca ccugugggca gacucaucac cgcgaacccc     1680

gugauuacgg aauccacaga gaauagcaag augaugcugg agcuugaucc cccuuucggg     1740

gacucuuaca ucguuauugg cgucggagag aaaaagauca cccaccacug gcaucgcucc     1800

ggcagcacaa uugggaaagc cuuugaagcu accgugcggg gagccaagag aauggcugug     1860

cugggcgaca ccgccuggga uuucgggucc gucggaggcg cccucaacuc ucuggggaaa     1920

ggcauccacc agaucuucgg agcugcguuu aagagccugu ucggcgggau guccugguuu     1980

ucucaaauuc uuaucggaac acuccugaug uggcugggcc ucaauacgaa gaacgggagc     2040

auuucccuga ugugucuugc ccugggaggc gugcucaucu uccugagcac cgcuguuucu     2100

gccgacgugg ggugc                                                      2115


<210>  2234
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2234
aucaacgcca gaaaggagaa aaagcggcgc ggcgcugaca ccagcguggg gauugucgga       60

cugcuccuga caacggccau ggcggcugaa guuaccagac ggggcuccgc cuacuauaug      120

uaccuugauc gcaaugacgc cggggaggcu aucucuuucc ccacaacccu gggaaugaac      180

aaaugcuaua uucagaucau ggaucucggc cacaugugug acgccacaau gagcuacgaa      240

ugcccuaugc uggaugaggg gguggaaccg gacgaugugg acuguuggug caauaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaagaaag gcgaggcuag acggucucgc      360

agagccguua cccuucccag ccauuccaca cggaagcugc aaacccgcuc ucagacaugg      420

cucgagagca gagaauacac caagcaccug auucgggugg agaacuggau cuuucgcaau      480

ccuggcuucg cgcuggccgc ugccgcuauc gccuggcucc ugggguccag cacgucucag      540

aaagucauuu aucuugugau gauccugcuc auugcgcccg ccuacuccau cagaugcauu      600

ggaguuagca accgggauuu uguggaaggc augucugggg gaacaugggu cgacaucgug      660

cuggagcaug gcgggugugu gaccgucaug gcucaggaua agccuacagu ugacauugag      720

cuugugacca ccacaguguc caauauggcc gaaguccgca gcuacugcua ugaggcuucu      780

aucuccgaua uggccagcga cuccagaugu cccacgcaag gagaagccua ccuggacaaa      840

cagucugaua cccaguaugu gugcaagcgg acacucguug acagaggcug gggcaacggg      900

uguggacugu ucggcaaagg gagccuggug accugcgcua aguuugcgug cuccaagaaa      960

augacaggaa agagcaucca gccugagaau cucgaauacc gcauuaugcu gucuguccac     1020

ggcucccaac acagcgggau gaucgugaac gauaccggac augagacgga cgagaaucgg     1080

gccaaaguug aaauuacacc gaacucuccc agagcugagg ccacccuugg cggcuucggg     1140

agccugggac ucgauuguga accucgcacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaua acaagcacug gcucgugcau aaagaguggu uccacgacau cccccugccu     1260

uggcacgccg gggcugauac cggaacaccc cauuggaaua acaaggaagc ccuggucgag     1320

uuuaaagacg cgcacgcuaa gcggcagacg guggucgugc ucggcucuca ggagggggcc     1380

guucauaccg cccuggcugg cgcccuugaa gcugagaugg auggagccaa gggcagacug     1440

agcuccgggc accucaaaug ccgccugaag auggacaaac uucggcugaa gggagugagc     1500

uauucucucu guacagcggc cuucaccuuu acaaaaauuc cggcugaaac ccugcauggc     1560

acggucaccg uggagguuca guacgccggg acagacggac cuugcaaggu gcccgcucaa     1620

auggccgucg auaugcagac ccugacaccu gugggcagac ucaucaccgc gaaccccgug     1680

aucacggaau ccacagagaa uagcaagaug augcuggagc uugauccccc uuucggggac     1740

ucuuacauug uuaucggcgu cggagagaaa aagauuaccc accacuggca ucgcuccggc     1800

agcacaaucg ggaaagccuu ugaagcuacc gugcggggag ccaagagaau ggcugugcug     1860

ggcgacaccg ccugggauuu cggguccguc ggaggcgccc ucaacucucu ggggaaaggc     1920

auucaccaga ucuucggagc ugcguuuaag agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua uuggaacacu ccugaugugg cugggccuca auacgaagaa cgggagcauc     2040

ucccugaugu gucuugcccu gggaggcgug cucauuuucc ugagcaccgc uguuucugcc     2100

gacguggggu gc                                                         2112


<210>  2235
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2235
aacgccagaa aggagaaaaa gcggcgcggc gcugacacca gcguggggau cgucggacug       60

cuccugacaa cggccauggc ggcugaaguu accagacggg gcuccgccua cuauauguac      120

cuugaucgca augacgccgg ggaggcuauu ucuuucccca caacccuggg aaugaacaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg ccacaaugag cuacgaaugc      240

ccuaugcugg augagggggu ggaaccggac gauguggacu guuggugcaa uaccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aggcuagacg gucucgcaga      360

gccguuaccc uucccagcca uuccacacgg aagcugcaaa cccgcucuca gacauggcuc      420

gagagcagag aauacaccaa gcaccugauc cggguggaga acuggauuuu ucgcaauccu      480

ggcuucgcgc uggccgcugc cgcuaucgcc uggcuccugg gguccagcac gucucagaaa      540

gucaucuauc uugugaugau ucugcucauc gcgcccgccu acuccauuag augcaucgga      600

guuagcaacc gggauuuugu ggaaggcaug ucugggggaa caugggucga cauugugcug      660

gagcauggcg ggugugugac cgucauggcu caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa uauggccgaa guccgcagcu acugcuauga ggcuucuauu      780

uccgauaugg ccagcgacuc cagauguccc acgcaaggag aagccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagcggaca cucguugaca gaggcugggg caacgggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgcuaagu uugcgugcuc caagaaaaug      960

acaggaaaga gcauccagcc ugagaaucuc gaauaccgca ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaacgau accggacaug agacggacga gaaucgggcc     1080

aaaguugaaa ucacaccgaa cucucccaga gcugaggcca cccuuggcgg cuucgggagc     1140

cugggacucg auugugaacc ucgcaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaauaaca agcacuggcu cgugcauaaa gagugguucc acgacauucc ccugccuugg     1260

cacgccgggg cugauaccgg aacaccccau uggaauaaca aggaagcccu ggucgaguuu     1320

aaagacgcgc acgcuaagcg gcagacggug gucgugcucg gcucucagga gggggccguu     1380

cauaccgccc uggcuggcgc ccuugaagcu gagauggaug gagccaaggg cagacugagc     1440

uccgggcacc ucaaaugccg ccugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucucucugua cagcggccuu caccuuuaca aaaaucccgg cugaaacccu gcauggcacg     1560

gucaccgugg agguucagua cgccgggaca gacggaccuu gcaaggugcc cgcucaaaug     1620

gccgucgaua ugcagacccu gacaccugug ggcagacuca uuaccgcgaa ccccgugauc     1680

acggaaucca cagagaauag caagaugaug cuggagcuug aucccccuuu cggggacucu     1740

uacaucguua uuggcgucgg agagaaaaag aucacccacc acuggcaucg cuccggcagc     1800

acaauuggga aagccuuuga agcuaccgug cggggagcca agagaauggc ugugcugggc     1860

gacaccgccu gggauuucgg guccgucgga ggcgcccuca acucucuggg gaaaggcauc     1920

caccagauuu ucggagcugc guuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaua cgaagaacgg gagcauuucc     2040

cugauguguc uugcccuggg aggcgugcuc aucuuccuga gcaccgcugu uucugccgac     2100

guggggugc                                                             2109


<210>  2236
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2236
gccagaaagg agaaaaagcg gcgcggcgcu gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg ccauggcggc ugaaguuacc agacggggcu ccgccuacua uauguaccuu      120

gaucgcaacg acgccgggga ggcuauuucu uuccccacaa cccugggaau gaauaaaugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcca caaugagcua cgaaugcccu      240

augcuggaug agggggugga accggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaag aaaggcgagg cuagacgguc ucgcagagcc      360

guuacccuuc ccagccauuc cacacggaag cugcaaaccc gcucucagac auggcucgag      420

agcagagaau acaccaagca ccugauccgg guggagaauu ggauuuuucg caacccuggc      480

uucgcgcugg ccgcugccgc uaucgccugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcg cccgccuacu ccauuagaug caucggaguu      600

agcaaucggg auuuugugga aggcaugucu gggggaacau gggucgacau ugugcuggag      660

cauggcgggu gugugaccgu cauggcucag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggccgaaguc cgcagcuacu gcuaugaggc uucuauuucc      780

gauauggcca gcgacuccag augucccacg caaggagaag ccuaccugga caaacagucu      840

gauacccagu augugugcaa gcggacacuc guugacagag gcuggggcaa ugggugugga      900

cuguucggca aagggagccu ggugaccugc gcuaaguuug cgugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga gaaccucgaa uaccgcauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaga cggacgagaa ccgggccaaa     1080

guugaaauca cacccaauuc ucccagagcu gaggccaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugaaccucg caccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaagc acuggcucgu gcauaaagag ugguuccacg acauuccccu gccuuggcac     1260

gccggggcug auaccggaac accccauugg aacaauaagg aagcccuggu cgaguuuaaa     1320

gacgcgcacg cuaagcggca gacggugguc gugcucggcu cucaggaggg ggccguucau     1380

accgcccugg cuggcgcccu ugaagcugag auggauggag ccaagggcag acugagcucc     1440

gggcaccuca aaugccgccu gaagauggac aaacuucggc ugaagggagu gagcuauucu     1500

cucuguacag cggccuucac cuuuacaaaa aucccggcug aaacccugca uggcacgguc     1560

accguggagg uucaguacgc cgggacagac ggaccuugca aggugcccgc ucaaauggcc     1620

gucgauaugc agacccugac accugugggc agacucauua ccgcgaaccc cgugaucacg     1680

gaauccacag agaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcaucgcuc cggcagcaca     1800

auugggaaag ccuuugaagc uaccgugcgg ggagccaaga gaauggcugu gcugggcgac     1860

accgccuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa aggcauccac     1920

cagauuuucg gagcugcguu uaagagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cccugggagg cgugcucauc uuccugagca ccgcuguuuc ugccgacgug     2100

gggugc                                                                2106


<210>  2237
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2237
agaaaggaga aaaagcggcg cggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga aguuaccaga cggggcuccg cuuacuauau guaccuugau      120

cgcaacgacg ccggggaggc cauuucuuuc cccacaaccc ugggaaugaa uaaaugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaagaaa ggcgaggcca gacggucucg cagagcuguu      360

acccuuccca gccauuccac acggaagcug caaacccgcu cucagacaug gcucgagagc      420

agagaauaca ccaagcaccu gauccgggug gagaauugga uuuuucgcaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uuagaugcau cggaguuagc      600

aaucgggauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaaguccgc agcuacugcu augaggccuc uauuuccgau      780

auggcuagcg acuccagaug ucccacgcaa ggagaagccu accuggacaa acagucugau      840

acccaguaug ugugcaagcg gacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaaag ggagccuggu gaccugcgcc aaguuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cgcaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgagaaccg ggcgaaaguu     1080

gaaaucacac ccaauucucc cagagccgag gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucgcac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaagcacu ggcucgugca uaaagagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaggaag cucuggucga guuuaaagac     1320

gcccacgcga agcggcagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga agccgagaug gauggagcua agggcagacu gagcuccggg     1440

caccucaaau gccgccugaa gauggacaaa cuucggcuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaaaauc ccggccgaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgcugg gacagacgga ccuugcaagg ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggcaga cucauuaccg ccaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgcuccgg cagcacaauu     1800

gggaaagcgu uugaagccac cgugcgggga gcuaagagaa uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaagg cauccaccag     1920

auuuucggag ccgcuuuuaa gagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacgugggg     2100

ugc                                                                   2103


<210>  2238
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2238
aaggagaaaa agagacgggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaagu uacccgcaga ggcuccgcuu acuauaugua ccuugaucgg      120

aacgacgccg gggaggccau uucuuucccc acaacccugg gaaugaauaa augcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgaaug cccuaugcug      240

gaugaggggg uggaaccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caagaaaggc gaggcccgca gaucucggcg cgcuguuacc      360

cuucccagcc auuccacaag aaagcugcaa acccggucuc agacauggcu cgagagccgc      420

gaauacacca agcaccugau cagaguggag aauuggauuu uucggaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauuc gcugcaucgg aguuagcaau      600

agagauuuug uggaaggcau gucuggggga acaugggucg acauugugcu ggagcauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga aguccggagc uacugcuaug aggccucuau uuccgauaug      780

gcuagcgacu cccgcugucc cacgcaagga gaagccuacc uggacaaaca gucugauacc      840

caguaugugu gcaagagaac acucguugac cggggcuggg gcaaugggug uggacuguuc      900

ggcaaaggga gccuggugac cugcgccaag uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugagaaccu cgaauacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gagacggacg agaaccgcgc gaaaguugaa     1080

aucacaccca auucuccccg ggccgaggcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugaac cuagaaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aagcacuggc ucgugcauaa agagugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaggaagcuc uggucgaguu uaaagacgcc     1320

cacgcgaagc gccagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaagc cgagauggau ggagcuaagg gccggcugag cuccgggcac     1440

cucaaaugca gacugaagau ggacaaacuu cgccugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaaaaucccg gccgaaaccc ugcauggcac ggucaccgug     1560

gagguucagu acgcugggac agacggaccu ugcaaggugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggccggcuc auuaccgcca accccgugau cacggaaucc     1680

acagagaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcaua gauccggcag cacaauuggg     1800

aaagcguuug aagccaccgu gcgcggagcu aagcggaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaaaggcau ccaccagauu     1920

uucggagccg cuuuuaagag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga cguggggugc     2100


<210>  2239
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2239
gagaagaaaa gacggggcgc cgacaccagc guggggaucg ucggacugcu ccugacaacg       60

gcuauggccg cggaaguuac ccgcagaggc uccgcuuacu auauguaccu ugaucggaac      120

gacgccgggg aggccauuuc uuuccccaca acccugggaa ugaauaagug cuauauccag      180

auuauggauc ucggccacau gugugacgcu acaaugagcu acgaaugccc uaugcuggau      240

gagggggugg aaccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uauggaacgu gucaucacaa aaagggcgag gcccgcagau cucggcgcgc uguuacccuu      360

cccagccauu ccacaagaaa acugcaaacc cggucucaga cauggcucga gagccgcgaa      420

uacaccaagc accugaucag aguggagaau uggauuuuuc ggaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggg uccagcacgu cucagaaggu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauucgcu gcaucggagu uagcaauaga      600

gauuuugugg aaggcauguc ugggggaaca ugggucgaca uugugcugga gcauggcggg      660

ugugugaccg ucauggccca ggauaaaccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaagu ccggagcuac ugcuaugagg ccucuauuuc cgauauggcu      780

agcgacuccc gcugucccac gcaaggagaa gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aaagaacacu cguugaccgg ggcuggggca augggugugg acuguucggc      900

aaggggagcc uggugaccug cgccaaauuu gcuugcucca agaagaugac aggaaaaagc      960

auccagccug agaaccucga auacagaauc augcugucug uccacggcuc ccaacacagc     1020

gggaugauug ugaaugauac cggacaugag acggacgaga accgcgcgaa gguugaaauc     1080

acacccaauu cuccccgggc cgaggcuacc cuuggcggcu ucgggagccu gggacucgau     1140

ugugaaccua gaaccggccu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga gugguuccac gacauucccc ugccuuggca cgccggggcc     1260

gauaccggaa caccccauug gaacaauaaa gaagcucugg ucgaguuuaa ggacgcccac     1320

gcgaaacgcc agacgguggu cgugcucggc ucucaggagg gggcuguuca uaccgcccug     1380

gccggcgcuc uugaagccga gauggaugga gcuaagggcc ggcugagcuc cgggcaccuc     1440

aagugcagac ugaaaaugga caaacuucgc cugaagggag ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gaaacccugc auggcacggu caccguggag     1560

guucaguacg cugggacaga cggaccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg ccggcucauu accgccaacc ccgugaucac ggaauccaca     1680

gagaacagca agaugaugcu ggagcuugac ccgccuuucg gggauucuua caucguuauu     1740

ggcgucggag aaaagaaaau cacccaccac uggcauagau ccggcagcac aauugggaag     1800

gcguuugagg ccaccgugcg cggagcuaaa cggauggccg ugcugggcga caccgcuugg     1860

gauuucgggu ccgucggagg cgcccucaau ucucugggga agggcaucca ccagauuuuc     1920

ggagccgcuu uuaaaagccu guucggcggg auguccuggu uuucucaaau ccuuaucgga     1980

acacuccuga uguggcuggg ccucaacacg aagaauggga gcauuucccu gaugugucuu     2040

gcgcugggag gcgugcucau cuuccugagc accgccguuu cugcugacgu ggggugc        2097


<210>  2240
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2240
aagaaaagac ggggcgccga caccagcgug gggaucgucg gacugcuccu gacaacggcu       60

auggccgcgg agguuacccg cagaggcucc gcuuacuaua uguaccuuga ucggaacgac      120

gccggggaag ccauuucuuu ccccacaacc cugggaauga auaagugcua uauccagauu      180

auggaucucg gccacaugug ugacgcuaca augagcuacg agugcccuau gcuggaugaa      240

gggguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

ggaacguguc aucacaaaaa gggcgaagcc cgcagaucuc ggcgcgcugu uacccuuccc      360

agccauucca caagaaaacu gcaaacccgg ucucagacau ggcucgagag ccgcgaguac      420

accaagcacc ugaucagagu ggaaaauugg auuuuucgga acccuggcuu cgcccuggcg      480

gccgcugcca ucgcuuggcu ccuggggucc agcacgucuc agaaggucau cuaucuugug      540

augauucugc ucaucgcccc cgcguacucc auucgcugca ucggaguuag caauagagau      600

uuuguggagg gcaugucugg gggaacaugg gucgacauug ugcuggaaca uggcgggugu      660

gugaccguca uggcccagga uaaaccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg cugagguccg gagcuacugc uaugaagccu cuauuuccga uauggcuagc      780

gacucccgcu gucccacgca aggagaggcc uaccuggaca agcagucuga uacccaguau      840

gugugcaaaa gaacacucgu ugaccggggc uggggcaaug gguguggacu guucggcaag      900

gggagccugg ugaccugcgc caaauuugcu ugcuccaaga agaugacagg aaaaagcauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggcuccca acacagcggg     1020

augauuguga augauaccgg acaugaaacg gacgagaacc gcgcgaaggu ugagaucaca     1080

cccaauucuc cccgggccga agcuacccuu ggcggcuucg ggagccuggg acucgauugu     1140

gagccuagaa ccggccugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc cggggccgau     1260

accggaacac cccauuggaa caauaaagag gcucuggucg aauuuaagga cgcccacgcg     1320

aaacgccaga cgguggucgu gcucggcucu caggaggggg cuguucauac cgcccuggcc     1380

ggcgcucuug aggccgaaau ggauggagcu aagggccggc ugagcuccgg gcaccucaag     1440

ugcagacuga aaauggacaa acuucgccug aagggaguga gcuauucucu cuguacagcc     1500

gcguucaccu uuacaaagau cccggccgag acccugcaug gcacggucac cguggaaguu     1560

caguacgcug ggacagacgg accuugcaaa gugcccgccc aaauggcugu cgauaugcag     1620

acccugacac cugugggccg gcucauuacc gccaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucgggg auucuuacau cguuauuggc     1740

gucggagaga agaaaaucac ccaccacugg cauagauccg gcagcacaau ugggaaggcg     1800

uuugaagcca ccgugcgcgg agcuaaacgg auggccgugc ugggcgacac cgcuugggau     1860

uucggguccg ucggaggcgc ccucaauucu cuggggaagg gcauccacca gauuuucgga     1920

gccgcuuuua aaagccuguu cggcgggaug uccugguuuu cucaaauccu uaucggaaca     1980

cuccugaugu ggcugggccu caacacgaag aaugggagca uuucccugau gugucuugcg     2040

cugggaggcg ugcucaucuu ccugagcacc gccguuucug cugacguggg gugc           2094


<210>  2241
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2241
aagagacggg gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug       60

gccgcggagg uuacccgcag aggcuccgcu uacuauaugu accuugaucg gaacgacgcc      120

ggggaagcca uuucuuuccc cacaacccug ggaaugaaua aaugcuauau ccagauuaug      180

gaucucggcc acauguguga cgcuacaaug agcuacgagu gcccuaugcu ggaugaaggg      240

guggagccgg acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga      300

acgugucauc acaagaaagg cgaagcccgc agaucucggc gcgcuguuac ccuucccagc      360

cauuccacaa gaaagcugca aacccggucu cagacauggc ucgagagccg cgaguacacc      420

aaacaccuga ucagagugga aaauuggauu uuucggaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggguccagc acgucucaga aggucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu cgcugcaucg gaguuagcaa uagagauuuu      600

guggagggca ugucuggggg aacauggguc gacauugugc uggaacaugg cgggugugug      660

accgucaugg cccaggauaa gccuacaguu gacaucgagc uugugaccac cacagugucc      720

aacauggcug agguccggag cuacugcuau gaagccucua uuuccgauau ggcuagcgac      780

ucccgcuguc ccacgcaagg agaggccuac cuggacaaac agucugauac ccaguaugug      840

ugcaagagaa cacucguuga ccggggcugg ggcaaugggu guggacuguu cggcaaaggg      900

agccugguga ccugcgccaa guuugcuugc uccaaaaaga ugacaggaaa gagcauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gcucccaaca cagcgggaug     1020

auugugaaug auaccggaca ugaaacggac gagaaccgcg cgaaaguuga gaucacaccc     1080

aauucucccc gggccgaagc uacccuuggc ggcuucggga gccugggacu cgauugugag     1140

ccuagaaccg gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaagcacugg     1200

cucgugcaua aagaaugguu ccacgacauu ccccugccuu ggcacgccgg ggccgauacc     1260

ggaacacccc auuggaacaa uaaggaggcu cuggucgaau uuaaagacgc ccacgcgaag     1320

cgccagacgg uggucgugcu cggcucucag gagggggcug uucauaccgc ccuggccggc     1380

gcucuugagg ccgaaaugga uggagcuaaa ggccggcuga gcuccgggca ccucaagugc     1440

agacugaaga uggacaaacu ucgccugaag ggagugagcu auucucucug uacagccgcg     1500

uucaccuuua caaaaauccc ggccgagacc cugcauggca cggucaccgu ggaaguucag     1560

uacgcuggga cagacggacc uugcaaggug cccgcccaaa uggcugucga uaugcagacc     1620

cugacaccug ugggccggcu cauuaccgcc aaccccguga ucacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugacccgccu uucggggauu cuuacaucgu uauuggcguc     1740

ggagagaaga agaucaccca ccacuggcau agauccggca gcacaauugg gaaagcguuu     1800

gaagccaccg ugcgcggagc uaagcggaug gccgugcugg gcgacaccgc uugggauuuc     1860

ggguccgucg gaggcgcccu caauucucug gggaaaggca uccaccagau uuucggagcc     1920

gcuuuuaaga gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc     1980

cugauguggc ugggccucaa cacgaaaaau gggagcauuu cccugaugug ucuugcgcug     2040

ggaggcgugc ucaucuuccu gagcaccgcc guuucugcug acguggggug c              2091


<210>  2242
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2242
agacggggcg ccgacaccag cguggggauc gucggacugc uccugacaac ggcuauggcc       60

gcggagguua cccgcagagg cuccgcuuac uauauguacc uugaucggaa cgacgccggg      120

gaagccauuu cuuuccccac aacccuggga augaauaagu gcuauaucca gauuauggau      180

cucggccaca ugugugacgc uacaaugagc uacgagugcc cuaugcugga ugaaggggug      240

gagccggacg auguggacug uuggugcaac accacaucca ccugggucgu guauggaacg      300

ugucaucaca aaaagggcga agcccgcaga ucucggcgcg cuguuacccu ucccagccau      360

uccacaagaa aacugcaaac ccggucucag acauggcucg agagccgcga guacaccaag      420

caccugauca gaguggaaaa uuggauuuuu cggaacccug gcuucgcccu ggcggccgcu      480

gccaucgcuu ggcuccuggg guccagcacg ucucagaaag ucaucuaucu ugugaugauu      540

cugcucaucg cccccgcgua cuccauucgc ugcaucggag uuagcaauag agauuuugug      600

gagggcaugu cugggggaac augggucgac auugugcugg aacauggcgg gugugugacc      660

gucauggccc aggauaagcc uacaguugac aucgagcuug ugaccaccac aguguccaac      720

auggcugagg uccggagcua cugcuaugaa gccucuauuu ccgauauggc uagcgacucc      780

cgcuguccca cgcaaggaga ggccuaccug gacaagcagu cugauaccca guaugugugc      840

aaaagaacac ucguugaccg gggcuggggc aaugggugug gacuguucgg caaggggagc      900

cuggugaccu gcgccaaauu ugcuugcucc aagaaaauga caggaaagag cauccagccu      960

gaaaaccucg aguacagaau caugcugucu guccacggcu cccaacacag cgggaugauu     1020

gugaaugaua ccggacauga aacggacgag aaccgcgcga agguugagau cacacccaau     1080

ucuccccggg ccgaagcuac ccuuggcggc uucgggagcc ugggacucga uugugagccu     1140

agaaccggcc uggacuuuuc cgaucuuuau uaccugacaa ugaacaauaa acacuggcuc     1200

gugcauaagg aaugguucca cgacauuccc cugccuuggc acgccggggc cgauaccgga     1260

acaccccauu ggaacaauaa agaggcucug gucgaauuua aggacgccca cgcgaaacgc     1320

cagacggugg ucgugcucgg cucucaggag ggggcuguuc auaccgcccu ggccggcgcu     1380

cuugaggccg aaauggaugg agcuaagggc cggcugagcu ccgggcaccu caaaugcaga     1440

cugaagaugg acaaacuucg ccugaaggga gugagcuauu cucucuguac agccgcguuc     1500

accuuuacaa agaucccggc cgagacccug cauggcacgg ucaccgugga aguucaguac     1560

gcugggacag acggaccuug caaagugccc gcccaaaugg cugucgauau gcagacccug     1620

acaccugugg gccggcucau uaccgccaac cccgugauca cggaguccac agaaaacagc     1680

aagaugaugc uggagcuuga cccgccuuuc ggggauucuu acaucguuau uggcgucgga     1740

gagaaaaaga ucacccacca cuggcauaga uccggcagca caauugggaa ggcguuugaa     1800

gccaccgugc gcggagcuaa acggauggcc gugcugggcg acaccgcuug ggauuucggg     1860

uccgucggag gcgcccucaa uucucugggg aagggcaucc accagauuuu cggagccgcu     1920

uuuaaaagcc uguucggcgg gauguccugg uuuucucaaa uccuuaucgg aacacuccug     1980

auguggcugg gccucaacac gaagaauggg agcauuuccc ugaugugucu ugcgcuggga     2040

ggcgugcuca ucuuccugag caccgccguu ucugcugacg uggggugc                  2088


<210>  2243
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2243
agaggcgccg acaccagcgu ggggaucguc ggacugcucc ugacaacggc uauggccgcg       60

gagguuaccc ggcgcggcuc cgcuuacuau auguaccuug auagaaacga cgccggggaa      120

gccauuucuu uccccacaac ccugggaaug aauaagugcu auauccagau uauggaucuc      180

ggccacaugu gugacgcuac aaugagcuac gagugcccua ugcuggauga agggguggag      240

ccggacgaug uggacuguug gugcaacacc acauccaccu gggucgugua uggaacgugu      300

caucacaaaa agggcgaagc ccggcgcucu agacgggcug uuacccuucc cagccauucc      360

acacgcaaac ugcaaaccag aucucagaca uggcucgaga gccgggagua caccaagcac      420

cugauccgcg uggaaaauug gauuuuuaga aacccuggcu ucgcccuggc ggccgcugcc      480

aucgcuuggc uccugggguc cagcacgucu cagaaaguca ucuaucuugu gaugauucug      540

cucaucgccc ccgcguacuc cauucggugc aucggaguua gcaaucgcga uuuuguggag      600

ggcaugucug ggggaacaug ggucgacauu gugcuggaac auggcgggug ugugaccguc      660

auggcccagg auaagccuac aguugacauc gagcuuguga ccaccacagu guccaacaug      720

gcugagguca gaagcuacug cuaugaagcc ucuauuuccg auauggcuag cgacucccgg      780

ugucccacgc aaggagaggc cuaccuggac aagcagucug auacccagua ugugugcaaa      840

cgcacacucg uugacagagg cuggggcaau ggguguggac uguucggcaa ggggagccug      900

gugaccugcg ccaaauuugc uugcuccaag aaaaugacag gaaagagcau ccagccugaa      960

aaccucgagu accggaucau gcugucuguc cacggcuccc aacacagcgg gaugauugug     1020

aaugauaccg gacaugaaac ggacgagaac agagcgaagg uugagaucac acccaauucu     1080

ccccgcgccg aagcuacccu uggcggcuuc gggagccugg gacucgauug ugagccucgg     1140

accggccugg acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug     1200

cauaaggaau gguuccacga cauuccccug ccuuggcacg ccggggccga uaccggaaca     1260

ccccauugga acaauaaaga ggcucugguc gaauuuaagg acgcccacgc gaaaagacag     1320

acgguggucg ugcucggcuc ucaggagggg gcuguucaua ccgcccuggc cggcgcucuu     1380

gaggccgaaa uggauggagc uaagggccgc cugagcuccg ggcaccucaa augccggcug     1440

aagauggaca aacuuagacu gaagggagug agcuauucuc ucuguacagc cgcguucacc     1500

uuuacaaaga ucccggccga gacccugcau ggcacgguca ccguggaagu ucaguacgcu     1560

gggacagacg gaccuugcaa agugcccgcc caaauggcug ucgauaugca gacccugaca     1620

ccugugggcc gccucauuac cgccaacccc gugaucacgg aguccacaga aaacagcaag     1680

augaugcugg agcuugaccc gccuuucggg gauucuuaca ucguuauugg cgucggagag     1740

aaaaagauca cccaccacug gcaucggucc ggcagcacaa uugggaaggc guuugaagcc     1800

accgugagag gagcuaaacg cauggccgug cugggcgaca ccgcuuggga uuucgggucc     1860

gucggaggcg cccucaauuc ucuggggaag ggcauccacc agauuuucgg agccgcuuuu     1920

aaaagccugu ucggcgggau guccugguuu ucucaaaucc uuaucggaac acuccugaug     1980

uggcugggcc ucaacacgaa gaaugggagc auuucccuga ugugucuugc gcugggaggc     2040

gugcucaucu uccugagcac cgccguuucu gcugacgugg ggugc                     2085


<210>  2244
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2244
ggcgccgaca ccagcguggg gaucgucgga cugcuccuga caacggcuau ggccgcggag       60

guuaccagac ggggcuccgc uuacuauaug uaccuugauc gcaacgacgc cggggaagcc      120

auuucuuucc ccacaacccu gggaaugaau aagugcuaua uccagauuau ggaucucggc      180

cacaugugug acgcuacaau gagcuacgag ugcccuaugc uggaugaagg gguggagccg      240

gacgaugugg acuguuggug caacaccaca uccaccuggg ucguguaugg aacgugucau      300

cacaaaaagg gcgaagccag acggucucgc agagcuguua cccuucccag ccauuccaca      360

cggaaacugc aaacccgcuc ucagacaugg cucgagagca gagaguacac caagcaccug      420

auccgggugg aaaauuggau uuuucgcaac ccuggcuucg cccuggcggc cgcugccauc      480

gcuuggcucc ugggguccag cacgucucag aaagucaucu aucuugugau gauucugcuc      540

aucgcccccg cguacuccau uagaugcauc ggaguuagca aucgggauuu uguggagggc      600

augucugggg gaacaugggu cgacauugug cuggaacaug gcgggugugu gaccgucaug      660

gcccaggaua agccuacagu ugacaucgag cuugugacca ccacaguguc caacauggcu      720

gagguccgca gcuacugcua ugaagccucu auuuccgaua uggcuagcga cuccagaugu      780

cccacgcaag gagaggccua ccuggacaag cagucugaua cccaguaugu gugcaaacgg      840

acacucguug accgcggcug gggcaauggg uguggacugu ucggcaaggg gagccuggug      900

accugcgcca aauuugcuug cuccaagaaa augacaggaa agagcaucca gccugaaaac      960

cucgaguaca gaaucaugcu gucuguccac ggcucccaac acagcgggau gauugugaau     1020

gauaccggac augaaacgga cgagaaccgg gcgaagguug agaucacacc caauucuccc     1080

agagccgaag cuacccuugg cggcuucggg agccugggac ucgauuguga gccucgcacc     1140

ggccuggacu uuuccgaucu uuauuaccug acaaugaaca auaaacacug gcucgugcau     1200

aaggaauggu uccacgacau uccccugccu uggcacgccg gggccgauac cggaacaccc     1260

cauuggaaca auaaagaggc ucuggucgaa uuuaaggacg cccacgcgaa acggcagacg     1320

guggucgugc ucggcucuca ggagggggcu guucauaccg cccuggccgg cgcucuugag     1380

gccgaaaugg auggagcuaa gggcagacug agcuccgggc accucaaaug ccgccugaag     1440

auggacaaac uucggcugaa gggagugagc uauucucucu guacagccgc guucaccuuu     1500

acaaagaucc cggccgagac ccugcauggc acggucaccg uggaaguuca guacgcuggg     1560

acagacggac cuugcaaagu gcccgcccaa auggcugucg auaugcagac ccugacaccu     1620

gugggcagac ucauuaccgc caaccccgug aucacggagu ccacagaaaa cagcaagaug     1680

augcuggagc uugacccgcc uuucggggau ucuuacaucg uuauuggcgu cggagagaaa     1740

aagaucaccc accacuggca ucgcuccggc agcacaauug ggaaggcguu ugaagccacc     1800

gugcggggag cuaaaagaau ggccgugcug ggcgacaccg cuugggauuu cggguccguc     1860

ggaggcgccc ucaauucucu ggggaagggc auccaccaga uuuucggagc cgcuuuuaaa     1920

agccuguucg gcgggauguc cugguuuucu caaauccuua ucggaacacu ccugaugugg     1980

cugggccuca acacgaagaa ugggagcauu ucccugaugu gucuugcgcu gggaggcgug     2040

cucaucuucc ugagcaccgc cguuucugcu gacguggggu gc                        2082


<210>  2245
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2245
gccgacacca gcgugggcau cgucgggcug cuccugacaa cggcuauggc cgcggagguu       60

accagacggg gauccgcuua cuauauguac cuugaucgca acgacgccgg cgaagccauu      120

ucuuucccca caacccuggg gaugaauaag ugcuauaucc agauuaugga ucucggacac      180

augugugacg cuacaaugag cuacgagugc ccuaugcugg augaaggcgu ggagccggac      240

gauguggacu guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac      300

aaaaagggag aagccagacg gucucgcaga gcuguuaccc uucccagcca uuccacacgg      360

aaacugcaaa cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc      420

cggguggaaa auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu      480

uggcuccugg gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc      540

gcccccgcgu acuccauuag augcaucggg guuagcaauc gggauuuugu ggagggaaug      600

ucuggcggga caugggucga cauugugcug gaacauggag gcugugugac cgucauggcc      660

caggauaagc cuacaguuga caucgagcuu gugaccacca caguguccaa cauggcugag      720

guccgcagcu acugcuauga agccucuauu uccgauaugg cuagcgacuc cagauguccc      780

acgcaagggg aggccuaccu ggacaagcag ucugauaccc aguaugugug caaacggaca      840

cucguugacc gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc      900

ugcgccaaau uugcuugcuc caagaaaaug acaggaaaga gcauccagcc ugaaaaccuc      960

gaguacagaa ucaugcuguc uguccacggg ucccaacaca gcggcaugau ugugaaugau     1020

accgggcaug aaacggacga gaaccgggcg aagguugaga ucacacccaa uucucccaga     1080

gccgaagcua cccuuggagg cuucggcagc cuggggcucg auugugagcc ucgcaccgga     1140

cuggacuuuu ccgaucuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag     1200

gaaugguucc acgacauucc ccugccuugg cacgccggcg ccgauaccgg gacaccccau     1260

uggaacaaua aagaggcucu ggucgaauuu aaggacgccc acgcgaaacg gcagacggug     1320

gucgugcucg gaucucagga gggcgcuguu cauaccgccc uggccggggc ucuugaggcc     1380

gaaauggaug gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug     1440

gacaagcugc ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca     1500

aagaucccgg ccgagacccu gcauggaacg gucaccgugg aaguucagua cgcuggcaca     1560

gacgggccuu gcaaagugcc cgcccaaaug gcugucgaua ugcagacccu gacaccugug     1620

ggaagacuca uuaccgccaa ccccgugauc acggagucca cagaaaacag caagaugaug     1680

cuggagcuug acccgccuuu cggcgauucu uacaucguua uuggggucgg cgagaaaaag     1740

aucacccacc acuggcaucg cuccggaagc acaauuggca aggcguuuga agccaccgug     1800

agaggggcua aacggauggc cgugcuggga gacaccgcuu gggauuucgg cuccgucggg     1860

ggagcccuca auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc     1920

cuguucggag gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug     1980

ggacucaaca cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc     2040

aucuuccuga gcaccgccgu uucugcugac gugggcugc                            2079


<210>  2246
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2246
gacaccagcg ugggcaucgu cgggcugcuc cugacaacgg ccauggcugc cgagguuacc       60

agacggggau ccgcguacua uauguaccuu gaucgcaacg acgcuggcga agccauuucu      120

uuccccacaa cccuggggau gaauaagugc uauauccaga uuauggaucu cggacacaug      180

ugugacgcca caaugagcua cgagugcccu augcuggaug aaggcgugga gccggacgau      240

guggacuguu ggugcaacac cacauccacc ugggucgugu augggacgug ucaucacaaa      300

aagggagaag cuagacgguc ucgcagagcc guuacccuuc ccagccauuc cacacggaaa      360

cugcaaaccc gcucucagac auggcucgag agcagagagu acaccaagca ccugauccgg      420

guggaaaauu ggauuuuucg caacccuggc uucgcucugg ccgcggccgc uaucgccugg      480

cuccugggcu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcu      540

cccgccuacu ccauuagaug caucgggguu agcaaucggg auuuugugga gggaaugucu      600

ggcgggacau gggucgacau ugugcuggaa cauggaggcu gugugaccgu cauggcgcag      660

gauaagccua caguugacau cgagcuugug accaccacag uguccaacau ggccgagguc      720

cgcagcuacu gcuaugaagc uagcauuucc gauauggcca gcgacuccag augucccacg      780

caaggggagg cuuaccugga caagcagucu gauacccagu augugugcaa acggacacuc      840

guugaccgcg gauggggcaa uggcuguggg cuguucggaa agggcagccu ggugaccugc      900

gccaaauuug ccugcuccaa gaaaaugaca gggaagucua uccagccuga aaaccucgag      960

uacagaauca ugcugucugu ccacggaucc caacacagcg gcaugauugu gaaugauacc     1020

gggcaugaaa cggacgagaa ccgggcuaag guugagauca cacccaauuc ucccagagcg     1080

gaagccaccc uuggaggcuu cggcagccug gggcucgauu gugagccucg caccggacug     1140

gacuuuuccg aucuuuauua ccugacaaug aacaauaaac acuggcucgu gcauaaggaa     1200

ugguuccacg acauuccccu gccuuggcac gcuggcgccg auaccgggac accccauugg     1260

aacaauaaag aggcccuggu cgaauuuaag gacgcucacg ccaaacggca gacggugguc     1320

gugcucggau cucaggaggg cgcgguucau accgcucugg ccggggcccu ugaggcugaa     1380

auggauggcg ccaagggaag acugagcucc ggccaccuca aaugccgccu gaagauggac     1440

aagcugcggc ugaaaggggu gagcuauucu cucuguacag cugccuucac cuuuacaaag     1500

aucccggcgg agacccugca uggaacgguc accguggaag uucaguacgc cggcacagac     1560

gggccuugca aagugcccgc ucaaauggcc gucgauaugc agacccugac accuguggga     1620

agacucauua ccgcuaaccc cgugaucacg gaguccacag aaaacagcaa gaugaugcug     1680

gagcuugacc cgccuuucgg cgauucuuac aucguuauug gggucggcga gaaaaagauc     1740

acccaccacu ggcaucgcuc cggaagcaca auuggcaagg ccuuugaagc gaccgugaga     1800

ggggccaaac ggauggcugu gcugggagac accgccuggg auuucggcuc cgucggggga     1860

gcucucaauu cucugggcaa ggggauccac cagauuuucg gcgccgccuu uaaaagccug     1920

uucggaggca uguccugguu uucucaaauc cuuaucggga cacuccugau guggcuggga     1980

cucaacacga agaauggcag cauuucccug augugucuug cucuuggggg agugcucauc     2040

uuccugagca ccgcgguuuc ugccgacgug ggcugc                               2076


<210>  2247
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2247
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc cgaugugggc ugc                                  2073


<210>  2248
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2248
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccgc gguuaccaga       60

cggggauccg cuuacuauau guaccuugac cgcaacgaug ccggcgaggc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugcuacaa ugagcuacga augcccuaug cuggacgagg gcguggaacc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaggcca gacggucucg cagagcuguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgaaagc agagaguaca ccaagcaccu gauccgggug      420

gagaauugga uuuuucgcaa cccuggcuuc gcccuggcgg ccgcugccau cgcuuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgccccc      540

gcguacucca uuagaugcau cgggguuagc aaucgggacu uuguggaagg aaugucuggc      600

gggacauggg ucgauauugu gcuggagcau ggaggcugug ugaccgucau ggcccaggac      660

aagccuacag uugauaucga acuugugacc accacagugu ccaacauggc ugagguccgc      720

agcuacugcu augaggccuc uauuuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaagccu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugcuu gcuccaagaa aaugacaggg aagucuaucc agccugagaa ccucgaauac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugagacgg augaaaaccg ggcgaagguu gagaucacac cgaauagccc cagagccgag     1080

gcuacccuug gaggcuucgg cagccugggg cucgacugug aaccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggagugg     1200

uuccacgaua uuccccugcc uuggcacgcc ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagaag cucuggucga guuuaaggau gcccacgcga aacggcagac gguggucgug     1320

cucggaucuc aggaaggcgc uguucauacc gcccuggccg gggcucuuga ggccgagaug     1380

gacggcgcua agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagccg cguucaccuu uacaaagauc     1500

ccggccgaaa cccugcaugg aacggucacc guggagguuc aguacgcugg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcuguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg ccaaccccgu gaucacggaa uccacagaga acagcaagau gaugcuggaa     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggcgu uugaggccac cgugagaggg     1800

gcuaaacgga uggccgugcu gggagauacc gcuugggacu ucggcuccgu cgggggagcc     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcgc uugggggagu gcucaucuuc     2040

cugagcaccg ccguuucugc ugaugugggc ugc                                  2073


<210>  2249
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2249
agaggcgccg acaccagcgu ggggaucguc ggacugcucc ugacaacggc uauggccgcg       60

gcuguuaccc ggcgcggcuc cgccuacuau auguaccuug auagaaacga cgccggggag      120

gcuauuucuu uccccacaac ccugggaaug aauaagugcu auauccagau uauggaucuc      180

ggccacaugu gugacgccac aaugagcuac gaaugcccua ugcuggauga ggggguggaa      240

ccggacgaug uggacuguug gugcaacacc acauccaccu gggucgugua uggaacgugu      300

caucacaaaa agggcgaggc ucggcgcucu agacgggccg uuacccuucc cagccauucc      360

acacgcaaac ugcaaaccag aucucagaca uggcucgaaa gccgggagua caccaagcac      420

cugauccgcg uggagaauug gauuuuuaga aacccuggcu ucgcgcuggc cgcugccgcu      480

aucgccuggc uccugggguc cagcacgucu cagaaaguca ucuaucuugu gaugauucug      540

cucaucgcgc ccgccuacuc cauucggugc aucggaguua gcaaucgcga uuuuguggaa      600

ggcaugucug ggggaacaug ggucgacauu gugcuggagc auggcgggug ugugaccguc      660

auggcucagg auaagccuac aguugacauc gaacuuguga ccaccacagu guccaacaug      720

gccgagguca gaagcuacug cuaugaggcu ucuauuuccg auauggccag cgacucccgg      780

ugucccacgc aaggagaagc cuaccuggac aagcagucug auacccagua ugugugcaaa      840

cgcacacucg uugacagagg cuggggcaau ggguguggac uguucggcaa ggggagccug      900

gugaccugcg cuaaauuugc gugcuccaag aaaaugacag gaaagagcau ccagccugag      960

aaccucgaau accggaucau gcugucuguc cacggcuccc aacacagcgg gaugauugug     1020

aaugauaccg gacaugagac ggacgaaaac agagccaagg uugagaucac acccaauucu     1080

ccccgcgcug aggccacccu uggcggcuuc gggagccugg gacucgauug ugaaccucgg     1140

accggccugg acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug     1200

cauaaggagu gguuccacga cauuccccug ccuuggcacg ccggggcuga uaccggaaca     1260

ccccauugga acaauaaaga agcccugguc gaguuuaagg acgcgcacgc uaaaagacag     1320

acgguggucg ugcucggcuc ucaggaaggg gccguucaua ccgcccuggc uggcgcccuu     1380

gaggcugaga uggauggagc caagggccgc cugagcuccg ggcaccucaa augccggcug     1440

aagauggaca aacuuagacu gaagggagug agcuauucuc ucuguacagc ggccuucacc     1500

uuuacaaaga ucccggcuga aacccugcau ggcacgguca ccguggaggu ucaguacgcc     1560

gggacagacg gaccuugcaa agugcccgcu caaauggccg ucgauaugca gacccugaca     1620

ccugugggcc gccucauuac cgcgaacccc gugaucacgg aauccacaga gaacagcaag     1680

augaugcugg aacuugaccc gccuuucggg gauucuuaca ucguuauugg cgucggagag     1740

aaaaagauca cccaccacug gcaucggucc ggcagcacaa uugggaaggc cuuugaggcu     1800

accgugagag gagccaaacg cauggcugug cugggcgaca ccgccuggga uuucgggucc     1860

gucggaggcg cccucaauuc ucuggggaag ggcauccacc agauuuucgg agcugcguuu     1920

aaaagccugu ucggcgggau guccugguuu ucucaaaucc uuaucggaac acuccugaug     1980

uggcugggcc ucaacacgaa gaaugggagc auuucccuga ugugucuugc ccugggaggc     2040

gugcucaucu uccugagcac cgcuguuucu gccgacgugg ggugc                     2085


<210>  2250
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2250
agaggccuga gcucccggaa gcgcagaucu cacgacgugc ucaccgucca guuccugauc       60

cuugggaugc ugcucaugac aggaggcguu acgcggcgcg ggagcgccua cuauauguac      120

cuggauagaa acgacgcugg agaggccauu uccuuuccca ccacacuugg caugaauaaa      180

ugcuauaucc aaauuaugga ucuggggcau augugugacg cgaccauguc uuacgaaugc      240

ccuaugcucg augagggagu ggaaccggac gauguggacu guuggugcaa cacaaccagc      300

acaugggucg uguauggcac cugucaccau aagaaaggcg aggcucggcg cuccagacgg      360

gccguuacgc ugcccucuca cagcacccgc aagcugcaga caagauccca gaccuggcuc      420

gaaagccggg aguacacaaa acaucugauc cgcguggaga auuggauuuu cagaaacccu      480

ggguuugccc uugcugccgc ugccaucgcg uggcugcucg gaucuuccac cagccagaag      540

gucaucuauc uggugaugau ucuucugauc gcccccgcuu acucuauucg gugcaucggc      600

guuuccaauc gcgauuucgu ggaagggaug agcggaggca cgugggucga cauugugcuc      660

gagcacgggg gaugugugac agucauggcc caagauaagc cuaccguuga caucgaacug      720

gugacaacca ccgugucuaa cauggcugag gucagauccu acugcuauga ggccagcauu      780

uccgauaugg cgucugacag ccgguguccc acacagggcg aagccuaccu ggacaaacag      840

uccgauacgc aguaugugug caagagaacc cucguugacc gcggcugggg gaauggaugu      900

ggccuguuug ggaaaggaag ccuugugaca ugcgcuaagu ucgccugcuc uaaaaagaug      960

accggcaagu ccauccaacc ugagaaccug gaauaccgga ucaugcucag cguccacggg     1020

ucucagcaua gcggaaugau ugugaaugau acaggccacg agaccgacga aaacagagcu     1080

aaaguugaga ucacgcccaa uuccccgcgc gccgaggcca cacugggcgg guuuggaucu     1140

cuuggccugg auugugaacc ucggaccggg cucgacuuca gcgaucugua uuaccugacc     1200

augaacaaua agcauuggcu cgugcacaaa gagugguuuc acgacauucc ccugccuugg     1260

caugcuggag cggauacagg cacaccccac uggaacaaua aggaagcccu ugucgaguuc     1320

aaagacgcuc augccaagag acagacagug gucgugcugg ggucccagga aggcgccguu     1380

cacacggcuc ucgccggagc gcuggaggcu gagauggaug gcgccaaagg gcgccuuagc     1440

ucuggacauc ugaagugccg gcucaagaug gacaaacuga gacugaaggg cguguccuau     1500

agccucugua ccgccgcuuu uacauucacc aaaaucccgg ccgaaacacu gcacgggacc     1560

gucacggugg agguucaaua cgcuggaacc gacggcccuu gcaaggugcc cgcccagaug     1620

gcggucgaua ugcagacacu uaccccugug gggcgccuga uuacagccaa ccccgugauc     1680

accgaaucua cggagaacuc caaaaugaug cucgaacugg aucccccuuu uggcgacagc     1740

uacaucguua uuggagucgg cgagaagaag aucacacacc auuggcaccg guccgggucu     1800

accauuggaa aggcuuucga ggccacagug agaggcgcua aacgcauggc cgugcugggg     1860

gacaccgcgu gggauuucgg aagcgucggc ggggcccuua auucccucgg caaaggaauc     1920

caucaaauuu uuggcgcugc cuucaagucu cuguuugggg gaaugagcug guucucccag     1980

auccugaucg gcacccuccu gauguggcuu gggcugaaca ccaaaaaugg aagcauuucu     2040

cucauguguc uggcucuggg cggggugcuu aucuuucucu ccacggccgu uagcgccgac     2100

gugggcugc                                                             2109


<210>  2251
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2251
agaggccuga gcucccggaa gcgcagaucu cacgacgugc ucaccgucca guuccugauc       60

cuugggaugc ugcucaugac aggagccguu acgcggcgcg gcagcgcuua cuauauguac      120

cuggauagaa acgacgccgg ggaggcgauu uccuuuccca ccacacuugg aaugaauaaa      180

ugcuauaucc aaauuaugga ucugggccau augugugacg cuaccauguc uuacgaaugc      240

ccuaugcucg augagggggu ggaaccggac gauguggacu guuggugcaa cacaaccagc      300

acaugggucg uguauggaac cugucaccau aagaaaggcg aggcccggcg cuccagacgg      360

gccguuacgc ugcccucuca cagcacccgc aagcugcaga caagauccca gaccuggcuc      420

gaaagccggg aguacacaaa acaucugauc cgcguggaga auuggauuuu cagaaacccu      480

ggcuuugcuc uugccgcugc cgcgaucgcc uggcugcucg ggucuuccac cagccagaag      540

gucaucuauc uggugaugau ucuucugauc gcucccgccu acucuauucg gugcaucgga      600

guuuccaauc gcgauuucgu ggaaggcaug agcgggggaa cgugggucga cauugugcuc      660

gagcacggcg ggugugugac agucauggcu caagauaagc cuaccguuga caucgaacug      720

gugacaacca ccgugucuaa cauggccgag gucagauccu acugcuauga ggcgagcauu      780

uccgauaugg ccucugacag ccgguguccc acacagggag aggcuuaccu ggacaaacag      840

uccgauacgc aguaugugug caagagaacc cucguugacc gcggcugggg caaugggugu      900

ggacuguuug gcaaagggag ccuugugaca ugcgccaagu ucgcuugcuc uaaaaagaug      960

accggaaagu ccauccaacc ugaaaaccug gaauaccgga ucaugcucag cguccacggc     1020

ucucagcaua gcgggaugau ugugaaugau acaggacacg agaccgacga aaacagagcc     1080

aaaguugaga ucacgcccaa uuccccgcgc gccgaggcua cacugggcgg cuuugggucu     1140

cuuggacugg auugugaacc ucggaccggc cucgacuuca gcgaucugua uuaccugacc     1200

augaacaaua agcauuggcu cgugcacaaa gagugguuuc acgacauucc ccugccuugg     1260

caugcggggg ccgauacagg aacaccccac uggaacaaua aggaagcucu ugucgaguuc     1320

aaagacgccc augccaagag acagacagug gucgugcugg gcucccagga aggggcuguu     1380

cacacggccc ucgcgggcgc ucuggaggcc gagauggaug gagccaaagg ccgccuuagc     1440

ucugggcauc ugaagugccg gcucaagaug gacaaacuga gacugaaggg aguguccuau     1500

agccucugua ccgcugccuu uacauucacc aaaaucccgg cugaaacacu gcacggcacc     1560

gucacggugg agguucaaua cgccgggacc gacggaccuu gcaaggugcc cgcgcagaug     1620

gccgucgaua ugcagacacu uaccccugug ggccgccuga uuacagcuaa ccccgugauc     1680

accgaaucua cggagaacuc caaaaugaug cucgaacugg aucccccuuu uggggacagc     1740

uacaucguua uuggcgucgg agagaagaag aucacacacc auuggcaccg guccggcucu     1800

accauuggga aagccuucga ggcuacagug agaggagcca agcgcauggc ggugcugggc     1860

gacaccgccu gggauuucgg gagcgucgga ggcgcucuua auucccucgg gaaaggcauc     1920

caucaaauuu uuggagccgc uuucaagucu cuguuuggcg ggaugagcug guucucccag     1980

auccugaucg gaacccuccu gauguggcuu ggccugaaca ccaaaaaugg gagcauuucu     2040

cucauguguc uggcccuggg aggcgugcuu aucuuucucu ccacggccgu uagcgcugac     2100

guggggugc                                                             2109


<210>  2252
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2252
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaau auggccgaag ucagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguugaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcgca cgcuaagaga cagacggugg ucgugcucgg cucucaggag     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccgugauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcgugcuca ucuuccugag caccgcuguu     2100

ucugccgac                                                             2109


<210>  2253
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2253
aucauuaacg ccagaaagga gaaaaagcgg cgcggcgcug acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc cauggcggcu gaaguuacca gacggggcuc cgccuacuau      120

auguaccuug aucgcaauga cgccggggag gcuauuucuu uccccacaac ccugggaaug      180

aacaaaugcu auauccagau uauggaucuc ggccacaugu gugacgccac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaauacc      300

acauccaccu gggucgugua uggaacgugu caucacaaga aaggcgaggc uagacggucu      360

cgcagagccg uuacccuucc cagccauucc acacggaagc ugcaaacccg cucucagaca      420

uggcucgaga gcagagaaua caccaagcac cugauccggg uggagaacug gaucuuucgc      480

aauccuggcu ucgcgcuggc cgcugccgcu auugccuggc uccugggguc cagcacgucu      540

cagaaaguca ucuaucuugu gaugauucug cucaucgcgc ccgccuacuc cauuagaugc      600

aucggaguua gcaaccggga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcucagg auaagccuac aguugacauc      720

gagcuuguga ccaccacagu guccaauaug gccgaagucc gcagcuacug cuaugaggcu      780

ucuaucuccg auauggccag cgacuccaga ugucccacgc aaggagaagc cuaccuggac      840

aaacagucug auacccagua ugugugcaag cggacacucg uugacagagg cuggggcaac      900

ggguguggac uguucggcaa agggagccug gugaccugcg cuaaguuugc gugcuccaag      960

aaaaugacag gaaagagcau ucagccugag aaucucgaau accgcaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aacgauaccg gacaugagac ggacgagaau     1080

cgggccaaag uugaaaucac accgaacucu cccagagcug aggccacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccucgc accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga auaacaagca cuggcucgug cauaaagagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggcuga uaccggaaca ccccauugga auaacaagga agcccugguc     1320

gaguuuaaag acgcgcacgc uaagcggcag acgguggucg ugcucggcuc ucaggagggg     1380

gccguucaua ccgcccuggc uggcgcccuu gaagcugaga uggauggagc caagggcaga     1440

cugagcuccg ggcaccucaa augccgccug aagauggaca aacuucggcu gaagggagug     1500

agcuauucuc ucuguacagc ggccuucacc uuuacaaaaa ucccggcuga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcc gggacagacg gaccuugcaa ggugcccgcu     1620

caaauggccg ucgauaugca gacccugaca ccugugggca gacucaucac cgcgaacccc     1680

gugauuacgg aauccacaga gaauagcaag augaugcugg agcuugaucc cccuuucggg     1740

gacucuuaca ucguuauugg cgucggagag aaaaagauca cccaccacug gcaucgcucc     1800

ggcagcacaa uugggaaagc cuuugaagcu accgugcggg gagccaagag aauggcugug     1860

cugggcgaca ccgccuggga uuucgggucc gucggaggcg cccucaacuc ucuggggaaa     1920

ggcauccacc agaucuucgg agcugcguuu aagagccugu ucggcgggau guccugguuu     1980

ucucaaauuc uuaucggaac acuccugaug uggcugggcc ucaauacgaa gaacgggagc     2040

auuucccuga ugugucuugc ccugggaggc gugcucaucu uccugagcac cgcuguuucu     2100

gccgac                                                                2106


<210>  2254
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2254
aucaacgcca gaaaggagaa aaagcggcgc ggcgcugaca ccagcguggg gauugucgga       60

cugcuccuga caacggccau ggcggcugaa guuaccagac ggggcuccgc cuacuauaug      120

uaccuugauc gcaaugacgc cggggaggcu aucucuuucc ccacaacccu gggaaugaac      180

aaaugcuaua uucagaucau ggaucucggc cacaugugug acgccacaau gagcuacgaa      240

ugcccuaugc uggaugaggg gguggaaccg gacgaugugg acuguuggug caauaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaagaaag gcgaggcuag acggucucgc      360

agagccguua cccuucccag ccauuccaca cggaagcugc aaacccgcuc ucagacaugg      420

cucgagagca gagaauacac caagcaccug auucgggugg agaacuggau cuuucgcaau      480

ccuggcuucg cgcuggccgc ugccgcuauc gccuggcucc ugggguccag cacgucucag      540

aaagucauuu aucuugugau gauccugcuc auugcgcccg ccuacuccau cagaugcauu      600

ggaguuagca accgggauuu uguggaaggc augucugggg gaacaugggu cgacaucgug      660

cuggagcaug gcgggugugu gaccgucaug gcucaggaua agccuacagu ugacauugag      720

cuugugacca ccacaguguc caauauggcc gaaguccgca gcuacugcua ugaggcuucu      780

aucuccgaua uggccagcga cuccagaugu cccacgcaag gagaagccua ccuggacaaa      840

cagucugaua cccaguaugu gugcaagcgg acacucguug acagaggcug gggcaacggg      900

uguggacugu ucggcaaagg gagccuggug accugcgcua aguuugcgug cuccaagaaa      960

augacaggaa agagcaucca gccugagaau cucgaauacc gcauuaugcu gucuguccac     1020

ggcucccaac acagcgggau gaucgugaac gauaccggac augagacgga cgagaaucgg     1080

gccaaaguug aaauuacacc gaacucuccc agagcugagg ccacccuugg cggcuucggg     1140

agccugggac ucgauuguga accucgcacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaua acaagcacug gcucgugcau aaagaguggu uccacgacau cccccugccu     1260

uggcacgccg gggcugauac cggaacaccc cauuggaaua acaaggaagc ccuggucgag     1320

uuuaaagacg cgcacgcuaa gcggcagacg guggucgugc ucggcucuca ggagggggcc     1380

guucauaccg cccuggcugg cgcccuugaa gcugagaugg auggagccaa gggcagacug     1440

agcuccgggc accucaaaug ccgccugaag auggacaaac uucggcugaa gggagugagc     1500

uauucucucu guacagcggc cuucaccuuu acaaaaauuc cggcugaaac ccugcauggc     1560

acggucaccg uggagguuca guacgccggg acagacggac cuugcaaggu gcccgcucaa     1620

auggccgucg auaugcagac ccugacaccu gugggcagac ucaucaccgc gaaccccgug     1680

aucacggaau ccacagagaa uagcaagaug augcuggagc uugauccccc uuucggggac     1740

ucuuacauug uuaucggcgu cggagagaaa aagauuaccc accacuggca ucgcuccggc     1800

agcacaaucg ggaaagccuu ugaagcuacc gugcggggag ccaagagaau ggcugugcug     1860

ggcgacaccg ccugggauuu cggguccguc ggaggcgccc ucaacucucu ggggaaaggc     1920

auucaccaga ucuucggagc ugcguuuaag agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua uuggaacacu ccugaugugg cugggccuca auacgaagaa cgggagcauc     2040

ucccugaugu gucuugcccu gggaggcgug cucauuuucc ugagcaccgc uguuucugcc     2100

gac                                                                   2103


<210>  2255
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2255
aacgccagaa aggagaaaaa gcggcgcggc gcugacacca gcguggggau cgucggacug       60

cuccugacaa cggccauggc ggcugaaguu accagacggg gcuccgccua cuauauguac      120

cuugaucgca augacgccgg ggaggcuauu ucuuucccca caacccuggg aaugaacaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg ccacaaugag cuacgaaugc      240

ccuaugcugg augagggggu ggaaccggac gauguggacu guuggugcaa uaccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aggcuagacg gucucgcaga      360

gccguuaccc uucccagcca uuccacacgg aagcugcaaa cccgcucuca gacauggcuc      420

gagagcagag aauacaccaa gcaccugauc cggguggaga acuggauuuu ucgcaauccu      480

ggcuucgcgc uggccgcugc cgcuaucgcc uggcuccugg gguccagcac gucucagaaa      540

gucaucuauc uugugaugau ucugcucauc gcgcccgccu acuccauuag augcaucgga      600

guuagcaacc gggauuuugu ggaaggcaug ucugggggaa caugggucga cauugugcug      660

gagcauggcg ggugugugac cgucauggcu caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa uauggccgaa guccgcagcu acugcuauga ggcuucuauu      780

uccgauaugg ccagcgacuc cagauguccc acgcaaggag aagccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagcggaca cucguugaca gaggcugggg caacgggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgcuaagu uugcgugcuc caagaaaaug      960

acaggaaaga gcauccagcc ugagaaucuc gaauaccgca ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaacgau accggacaug agacggacga gaaucgggcc     1080

aaaguugaaa ucacaccgaa cucucccaga gcugaggcca cccuuggcgg cuucgggagc     1140

cugggacucg auugugaacc ucgcaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaauaaca agcacuggcu cgugcauaaa gagugguucc acgacauucc ccugccuugg     1260

cacgccgggg cugauaccgg aacaccccau uggaauaaca aggaagcccu ggucgaguuu     1320

aaagacgcgc acgcuaagcg gcagacggug gucgugcucg gcucucagga gggggccguu     1380

cauaccgccc uggcuggcgc ccuugaagcu gagauggaug gagccaaggg cagacugagc     1440

uccgggcacc ucaaaugccg ccugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucucucugua cagcggccuu caccuuuaca aaaaucccgg cugaaacccu gcauggcacg     1560

gucaccgugg agguucagua cgccgggaca gacggaccuu gcaaggugcc cgcucaaaug     1620

gccgucgaua ugcagacccu gacaccugug ggcagacuca uuaccgcgaa ccccgugauc     1680

acggaaucca cagagaauag caagaugaug cuggagcuug aucccccuuu cggggacucu     1740

uacaucguua uuggcgucgg agagaaaaag aucacccacc acuggcaucg cuccggcagc     1800

acaauuggga aagccuuuga agcuaccgug cggggagcca agagaauggc ugugcugggc     1860

gacaccgccu gggauuucgg guccgucgga ggcgcccuca acucucuggg gaaaggcauc     1920

caccagauuu ucggagcugc guuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaua cgaagaacgg gagcauuucc     2040

cugauguguc uugcccuggg aggcgugcuc aucuuccuga gcaccgcugu uucugccgac     2100


<210>  2256
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2256
gccagaaagg agaaaaagcg gcgcggcgcu gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg ccauggcggc ugaaguuacc agacggggcu ccgccuacua uauguaccuu      120

gaucgcaacg acgccgggga ggcuauuucu uuccccacaa cccugggaau gaauaaaugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcca caaugagcua cgaaugcccu      240

augcuggaug agggggugga accggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaag aaaggcgagg cuagacgguc ucgcagagcc      360

guuacccuuc ccagccauuc cacacggaag cugcaaaccc gcucucagac auggcucgag      420

agcagagaau acaccaagca ccugauccgg guggagaauu ggauuuuucg caacccuggc      480

uucgcgcugg ccgcugccgc uaucgccugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcg cccgccuacu ccauuagaug caucggaguu      600

agcaaucggg auuuugugga aggcaugucu gggggaacau gggucgacau ugugcuggag      660

cauggcgggu gugugaccgu cauggcucag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggccgaaguc cgcagcuacu gcuaugaggc uucuauuucc      780

gauauggcca gcgacuccag augucccacg caaggagaag ccuaccugga caaacagucu      840

gauacccagu augugugcaa gcggacacuc guugacagag gcuggggcaa ugggugugga      900

cuguucggca aagggagccu ggugaccugc gcuaaguuug cgugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga gaaccucgaa uaccgcauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaga cggacgagaa ccgggccaaa     1080

guugaaauca cacccaauuc ucccagagcu gaggccaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugaaccucg caccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaagc acuggcucgu gcauaaagag ugguuccacg acauuccccu gccuuggcac     1260

gccggggcug auaccggaac accccauugg aacaauaagg aagcccuggu cgaguuuaaa     1320

gacgcgcacg cuaagcggca gacggugguc gugcucggcu cucaggaggg ggccguucau     1380

accgcccugg cuggcgcccu ugaagcugag auggauggag ccaagggcag acugagcucc     1440

gggcaccuca aaugccgccu gaagauggac aaacuucggc ugaagggagu gagcuauucu     1500

cucuguacag cggccuucac cuuuacaaaa aucccggcug aaacccugca uggcacgguc     1560

accguggagg uucaguacgc cgggacagac ggaccuugca aggugcccgc ucaaauggcc     1620

gucgauaugc agacccugac accugugggc agacucauua ccgcgaaccc cgugaucacg     1680

gaauccacag agaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcaucgcuc cggcagcaca     1800

auugggaaag ccuuugaagc uaccgugcgg ggagccaaga gaauggcugu gcugggcgac     1860

accgccuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa aggcauccac     1920

cagauuuucg gagcugcguu uaagagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cccugggagg cgugcucauc uuccugagca ccgcuguuuc ugccgac        2097


<210>  2257
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2257
agaaaggaga aaaagcggcg cggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga aguuaccaga cggggcuccg cuuacuauau guaccuugau      120

cgcaacgacg ccggggaggc cauuucuuuc cccacaaccc ugggaaugaa uaaaugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaagaaa ggcgaggcca gacggucucg cagagcuguu      360

acccuuccca gccauuccac acggaagcug caaacccgcu cucagacaug gcucgagagc      420

agagaauaca ccaagcaccu gauccgggug gagaauugga uuuuucgcaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uuagaugcau cggaguuagc      600

aaucgggauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaaguccgc agcuacugcu augaggccuc uauuuccgau      780

auggcuagcg acuccagaug ucccacgcaa ggagaagccu accuggacaa acagucugau      840

acccaguaug ugugcaagcg gacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaaag ggagccuggu gaccugcgcc aaguuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cgcaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgagaaccg ggcgaaaguu     1080

gaaaucacac ccaauucucc cagagccgag gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucgcac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaagcacu ggcucgugca uaaagagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaggaag cucuggucga guuuaaagac     1320

gcccacgcga agcggcagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga agccgagaug gauggagcua agggcagacu gagcuccggg     1440

caccucaaau gccgccugaa gauggacaaa cuucggcuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaaaauc ccggccgaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgcugg gacagacgga ccuugcaagg ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggcaga cucauuaccg ccaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgcuccgg cagcacaauu     1800

gggaaagcgu uugaagccac cgugcgggga gcuaagagaa uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaagg cauccaccag     1920

auuuucggag ccgcuuuuaa gagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugac           2094


<210>  2258
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2258
aaggagaaaa agagacgggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaagu uacccgcaga ggcuccgcuu acuauaugua ccuugaucgg      120

aacgacgccg gggaggccau uucuuucccc acaacccugg gaaugaauaa augcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgaaug cccuaugcug      240

gaugaggggg uggaaccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caagaaaggc gaggcccgca gaucucggcg cgcuguuacc      360

cuucccagcc auuccacaag aaagcugcaa acccggucuc agacauggcu cgagagccgc      420

gaauacacca agcaccugau cagaguggag aauuggauuu uucggaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauuc gcugcaucgg aguuagcaau      600

agagauuuug uggaaggcau gucuggggga acaugggucg acauugugcu ggagcauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga aguccggagc uacugcuaug aggccucuau uuccgauaug      780

gcuagcgacu cccgcugucc cacgcaagga gaagccuacc uggacaaaca gucugauacc      840

caguaugugu gcaagagaac acucguugac cggggcuggg gcaaugggug uggacuguuc      900

ggcaaaggga gccuggugac cugcgccaag uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugagaaccu cgaauacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gagacggacg agaaccgcgc gaaaguugaa     1080

aucacaccca auucuccccg ggccgaggcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugaac cuagaaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aagcacuggc ucgugcauaa agagugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaggaagcuc uggucgaguu uaaagacgcc     1320

cacgcgaagc gccagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaagc cgagauggau ggagcuaagg gccggcugag cuccgggcac     1440

cucaaaugca gacugaagau ggacaaacuu cgccugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaaaaucccg gccgaaaccc ugcauggcac ggucaccgug     1560

gagguucagu acgcugggac agacggaccu ugcaaggugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggccggcuc auuaccgcca accccgugau cacggaaucc     1680

acagagaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcaua gauccggcag cacaauuggg     1800

aaagcguuug aagccaccgu gcgcggagcu aagcggaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaaaggcau ccaccagauu     1920

uucggagccg cuuuuaagag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga c              2091


<210>  2259
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2259
gagaagaaaa gacggggcgc cgacaccagc guggggaucg ucggacugcu ccugacaacg       60

gcuauggccg cggaaguuac ccgcagaggc uccgcuuacu auauguaccu ugaucggaac      120

gacgccgggg aggccauuuc uuuccccaca acccugggaa ugaauaagug cuauauccag      180

auuauggauc ucggccacau gugugacgcu acaaugagcu acgaaugccc uaugcuggau      240

gagggggugg aaccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uauggaacgu gucaucacaa aaagggcgag gcccgcagau cucggcgcgc uguuacccuu      360

cccagccauu ccacaagaaa acugcaaacc cggucucaga cauggcucga gagccgcgaa      420

uacaccaagc accugaucag aguggagaau uggauuuuuc ggaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggg uccagcacgu cucagaaggu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauucgcu gcaucggagu uagcaauaga      600

gauuuugugg aaggcauguc ugggggaaca ugggucgaca uugugcugga gcauggcggg      660

ugugugaccg ucauggccca ggauaaaccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaagu ccggagcuac ugcuaugagg ccucuauuuc cgauauggcu      780

agcgacuccc gcugucccac gcaaggagaa gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aaagaacacu cguugaccgg ggcuggggca augggugugg acuguucggc      900

aaggggagcc uggugaccug cgccaaauuu gcuugcucca agaagaugac aggaaaaagc      960

auccagccug agaaccucga auacagaauc augcugucug uccacggcuc ccaacacagc     1020

gggaugauug ugaaugauac cggacaugag acggacgaga accgcgcgaa gguugaaauc     1080

acacccaauu cuccccgggc cgaggcuacc cuuggcggcu ucgggagccu gggacucgau     1140

ugugaaccua gaaccggccu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga gugguuccac gacauucccc ugccuuggca cgccggggcc     1260

gauaccggaa caccccauug gaacaauaaa gaagcucugg ucgaguuuaa ggacgcccac     1320

gcgaaacgcc agacgguggu cgugcucggc ucucaggagg gggcuguuca uaccgcccug     1380

gccggcgcuc uugaagccga gauggaugga gcuaagggcc ggcugagcuc cgggcaccuc     1440

aagugcagac ugaaaaugga caaacuucgc cugaagggag ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gaaacccugc auggcacggu caccguggag     1560

guucaguacg cugggacaga cggaccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg ccggcucauu accgccaacc ccgugaucac ggaauccaca     1680

gagaacagca agaugaugcu ggagcuugac ccgccuuucg gggauucuua caucguuauu     1740

ggcgucggag aaaagaaaau cacccaccac uggcauagau ccggcagcac aauugggaag     1800

gcguuugagg ccaccgugcg cggagcuaaa cggauggccg ugcugggcga caccgcuugg     1860

gauuucgggu ccgucggagg cgcccucaau ucucugggga agggcaucca ccagauuuuc     1920

ggagccgcuu uuaaaagccu guucggcggg auguccuggu uuucucaaau ccuuaucgga     1980

acacuccuga uguggcuggg ccucaacacg aagaauggga gcauuucccu gaugugucuu     2040

gcgcugggag gcgugcucau cuuccugagc accgccguuu cugcugac                  2088


<210>  2260
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2260
aagaaaagac ggggcgccga caccagcgug gggaucgucg gacugcuccu gacaacggcu       60

auggccgcgg agguuacccg cagaggcucc gcuuacuaua uguaccuuga ucggaacgac      120

gccggggaag ccauuucuuu ccccacaacc cugggaauga auaagugcua uauccagauu      180

auggaucucg gccacaugug ugacgcuaca augagcuacg agugcccuau gcuggaugaa      240

gggguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

ggaacguguc aucacaaaaa gggcgaagcc cgcagaucuc ggcgcgcugu uacccuuccc      360

agccauucca caagaaaacu gcaaacccgg ucucagacau ggcucgagag ccgcgaguac      420

accaagcacc ugaucagagu ggaaaauugg auuuuucgga acccuggcuu cgcccuggcg      480

gccgcugcca ucgcuuggcu ccuggggucc agcacgucuc agaaggucau cuaucuugug      540

augauucugc ucaucgcccc cgcguacucc auucgcugca ucggaguuag caauagagau      600

uuuguggagg gcaugucugg gggaacaugg gucgacauug ugcuggaaca uggcgggugu      660

gugaccguca uggcccagga uaaaccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg cugagguccg gagcuacugc uaugaagccu cuauuuccga uauggcuagc      780

gacucccgcu gucccacgca aggagaggcc uaccuggaca agcagucuga uacccaguau      840

gugugcaaaa gaacacucgu ugaccggggc uggggcaaug gguguggacu guucggcaag      900

gggagccugg ugaccugcgc caaauuugcu ugcuccaaga agaugacagg aaaaagcauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggcuccca acacagcggg     1020

augauuguga augauaccgg acaugaaacg gacgagaacc gcgcgaaggu ugagaucaca     1080

cccaauucuc cccgggccga agcuacccuu ggcggcuucg ggagccuggg acucgauugu     1140

gagccuagaa ccggccugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc cggggccgau     1260

accggaacac cccauuggaa caauaaagag gcucuggucg aauuuaagga cgcccacgcg     1320

aaacgccaga cgguggucgu gcucggcucu caggaggggg cuguucauac cgcccuggcc     1380

ggcgcucuug aggccgaaau ggauggagcu aagggccggc ugagcuccgg gcaccucaag     1440

ugcagacuga aaauggacaa acuucgccug aagggaguga gcuauucucu cuguacagcc     1500

gcguucaccu uuacaaagau cccggccgag acccugcaug gcacggucac cguggaaguu     1560

caguacgcug ggacagacgg accuugcaaa gugcccgccc aaauggcugu cgauaugcag     1620

acccugacac cugugggccg gcucauuacc gccaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucgggg auucuuacau cguuauuggc     1740

gucggagaga agaaaaucac ccaccacugg cauagauccg gcagcacaau ugggaaggcg     1800

uuugaagcca ccgugcgcgg agcuaaacgg auggccgugc ugggcgacac cgcuugggau     1860

uucggguccg ucggaggcgc ccucaauucu cuggggaagg gcauccacca gauuuucgga     1920

gccgcuuuua aaagccuguu cggcgggaug uccugguuuu cucaaauccu uaucggaaca     1980

cuccugaugu ggcugggccu caacacgaag aaugggagca uuucccugau gugucuugcg     2040

cugggaggcg ugcucaucuu ccugagcacc gccguuucug cugac                     2085


<210>  2261
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2261
aagagacggg gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug       60

gccgcggagg uuacccgcag aggcuccgcu uacuauaugu accuugaucg gaacgacgcc      120

ggggaagcca uuucuuuccc cacaacccug ggaaugaaua aaugcuauau ccagauuaug      180

gaucucggcc acauguguga cgcuacaaug agcuacgagu gcccuaugcu ggaugaaggg      240

guggagccgg acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga      300

acgugucauc acaagaaagg cgaagcccgc agaucucggc gcgcuguuac ccuucccagc      360

cauuccacaa gaaagcugca aacccggucu cagacauggc ucgagagccg cgaguacacc      420

aaacaccuga ucagagugga aaauuggauu uuucggaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggguccagc acgucucaga aggucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu cgcugcaucg gaguuagcaa uagagauuuu      600

guggagggca ugucuggggg aacauggguc gacauugugc uggaacaugg cgggugugug      660

accgucaugg cccaggauaa gccuacaguu gacaucgagc uugugaccac cacagugucc      720

aacauggcug agguccggag cuacugcuau gaagccucua uuuccgauau ggcuagcgac      780

ucccgcuguc ccacgcaagg agaggccuac cuggacaaac agucugauac ccaguaugug      840

ugcaagagaa cacucguuga ccggggcugg ggcaaugggu guggacuguu cggcaaaggg      900

agccugguga ccugcgccaa guuugcuugc uccaaaaaga ugacaggaaa gagcauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gcucccaaca cagcgggaug     1020

auugugaaug auaccggaca ugaaacggac gagaaccgcg cgaaaguuga gaucacaccc     1080

aauucucccc gggccgaagc uacccuuggc ggcuucggga gccugggacu cgauugugag     1140

ccuagaaccg gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaagcacugg     1200

cucgugcaua aagaaugguu ccacgacauu ccccugccuu ggcacgccgg ggccgauacc     1260

ggaacacccc auuggaacaa uaaggaggcu cuggucgaau uuaaagacgc ccacgcgaag     1320

cgccagacgg uggucgugcu cggcucucag gagggggcug uucauaccgc ccuggccggc     1380

gcucuugagg ccgaaaugga uggagcuaaa ggccggcuga gcuccgggca ccucaagugc     1440

agacugaaga uggacaaacu ucgccugaag ggagugagcu auucucucug uacagccgcg     1500

uucaccuuua caaaaauccc ggccgagacc cugcauggca cggucaccgu ggaaguucag     1560

uacgcuggga cagacggacc uugcaaggug cccgcccaaa uggcugucga uaugcagacc     1620

cugacaccug ugggccggcu cauuaccgcc aaccccguga ucacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugacccgccu uucggggauu cuuacaucgu uauuggcguc     1740

ggagagaaga agaucaccca ccacuggcau agauccggca gcacaauugg gaaagcguuu     1800

gaagccaccg ugcgcggagc uaagcggaug gccgugcugg gcgacaccgc uugggauuuc     1860

ggguccgucg gaggcgcccu caauucucug gggaaaggca uccaccagau uuucggagcc     1920

gcuuuuaaga gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc     1980

cugauguggc ugggccucaa cacgaaaaau gggagcauuu cccugaugug ucuugcgcug     2040

ggaggcgugc ucaucuuccu gagcaccgcc guuucugcug ac                        2082


<210>  2262
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2262
agacggggcg ccgacaccag cguggggauc gucggacugc uccugacaac ggcuauggcc       60

gcggagguua cccgcagagg cuccgcuuac uauauguacc uugaucggaa cgacgccggg      120

gaagccauuu cuuuccccac aacccuggga augaauaagu gcuauaucca gauuauggau      180

cucggccaca ugugugacgc uacaaugagc uacgagugcc cuaugcugga ugaaggggug      240

gagccggacg auguggacug uuggugcaac accacaucca ccugggucgu guauggaacg      300

ugucaucaca aaaagggcga agcccgcaga ucucggcgcg cuguuacccu ucccagccau      360

uccacaagaa aacugcaaac ccggucucag acauggcucg agagccgcga guacaccaag      420

caccugauca gaguggaaaa uuggauuuuu cggaacccug gcuucgcccu ggcggccgcu      480

gccaucgcuu ggcuccuggg guccagcacg ucucagaaag ucaucuaucu ugugaugauu      540

cugcucaucg cccccgcgua cuccauucgc ugcaucggag uuagcaauag agauuuugug      600

gagggcaugu cugggggaac augggucgac auugugcugg aacauggcgg gugugugacc      660

gucauggccc aggauaagcc uacaguugac aucgagcuug ugaccaccac aguguccaac      720

auggcugagg uccggagcua cugcuaugaa gccucuauuu ccgauauggc uagcgacucc      780

cgcuguccca cgcaaggaga ggccuaccug gacaagcagu cugauaccca guaugugugc      840

aaaagaacac ucguugaccg gggcuggggc aaugggugug gacuguucgg caaggggagc      900

cuggugaccu gcgccaaauu ugcuugcucc aagaaaauga caggaaagag cauccagccu      960

gaaaaccucg aguacagaau caugcugucu guccacggcu cccaacacag cgggaugauu     1020

gugaaugaua ccggacauga aacggacgag aaccgcgcga agguugagau cacacccaau     1080

ucuccccggg ccgaagcuac ccuuggcggc uucgggagcc ugggacucga uugugagccu     1140

agaaccggcc uggacuuuuc cgaucuuuau uaccugacaa ugaacaauaa acacuggcuc     1200

gugcauaagg aaugguucca cgacauuccc cugccuuggc acgccggggc cgauaccgga     1260

acaccccauu ggaacaauaa agaggcucug gucgaauuua aggacgccca cgcgaaacgc     1320

cagacggugg ucgugcucgg cucucaggag ggggcuguuc auaccgcccu ggccggcgcu     1380

cuugaggccg aaauggaugg agcuaagggc cggcugagcu ccgggcaccu caaaugcaga     1440

cugaagaugg acaaacuucg ccugaaggga gugagcuauu cucucuguac agccgcguuc     1500

accuuuacaa agaucccggc cgagacccug cauggcacgg ucaccgugga aguucaguac     1560

gcugggacag acggaccuug caaagugccc gcccaaaugg cugucgauau gcagacccug     1620

acaccugugg gccggcucau uaccgccaac cccgugauca cggaguccac agaaaacagc     1680

aagaugaugc uggagcuuga cccgccuuuc ggggauucuu acaucguuau uggcgucgga     1740

gagaaaaaga ucacccacca cuggcauaga uccggcagca caauugggaa ggcguuugaa     1800

gccaccgugc gcggagcuaa acggauggcc gugcugggcg acaccgcuug ggauuucggg     1860

uccgucggag gcgcccucaa uucucugggg aagggcaucc accagauuuu cggagccgcu     1920

uuuaaaagcc uguucggcgg gauguccugg uuuucucaaa uccuuaucgg aacacuccug     1980

auguggcugg gccucaacac gaagaauggg agcauuuccc ugaugugucu ugcgcuggga     2040

ggcgugcuca ucuuccugag caccgccguu ucugcugac                            2079


<210>  2263
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2263
agaggcgccg acaccagcgu ggggaucguc ggacugcucc ugacaacggc uauggccgcg       60

gagguuaccc ggcgcggcuc cgcuuacuau auguaccuug auagaaacga cgccggggaa      120

gccauuucuu uccccacaac ccugggaaug aauaagugcu auauccagau uauggaucuc      180

ggccacaugu gugacgcuac aaugagcuac gagugcccua ugcuggauga agggguggag      240

ccggacgaug uggacuguug gugcaacacc acauccaccu gggucgugua uggaacgugu      300

caucacaaaa agggcgaagc ccggcgcucu agacgggcug uuacccuucc cagccauucc      360

acacgcaaac ugcaaaccag aucucagaca uggcucgaga gccgggagua caccaagcac      420

cugauccgcg uggaaaauug gauuuuuaga aacccuggcu ucgcccuggc ggccgcugcc      480

aucgcuuggc uccugggguc cagcacgucu cagaaaguca ucuaucuugu gaugauucug      540

cucaucgccc ccgcguacuc cauucggugc aucggaguua gcaaucgcga uuuuguggag      600

ggcaugucug ggggaacaug ggucgacauu gugcuggaac auggcgggug ugugaccguc      660

auggcccagg auaagccuac aguugacauc gagcuuguga ccaccacagu guccaacaug      720

gcugagguca gaagcuacug cuaugaagcc ucuauuuccg auauggcuag cgacucccgg      780

ugucccacgc aaggagaggc cuaccuggac aagcagucug auacccagua ugugugcaaa      840

cgcacacucg uugacagagg cuggggcaau ggguguggac uguucggcaa ggggagccug      900

gugaccugcg ccaaauuugc uugcuccaag aaaaugacag gaaagagcau ccagccugaa      960

aaccucgagu accggaucau gcugucuguc cacggcuccc aacacagcgg gaugauugug     1020

aaugauaccg gacaugaaac ggacgagaac agagcgaagg uugagaucac acccaauucu     1080

ccccgcgccg aagcuacccu uggcggcuuc gggagccugg gacucgauug ugagccucgg     1140

accggccugg acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug     1200

cauaaggaau gguuccacga cauuccccug ccuuggcacg ccggggccga uaccggaaca     1260

ccccauugga acaauaaaga ggcucugguc gaauuuaagg acgcccacgc gaaaagacag     1320

acgguggucg ugcucggcuc ucaggagggg gcuguucaua ccgcccuggc cggcgcucuu     1380

gaggccgaaa uggauggagc uaagggccgc cugagcuccg ggcaccucaa augccggcug     1440

aagauggaca aacuuagacu gaagggagug agcuauucuc ucuguacagc cgcguucacc     1500

uuuacaaaga ucccggccga gacccugcau ggcacgguca ccguggaagu ucaguacgcu     1560

gggacagacg gaccuugcaa agugcccgcc caaauggcug ucgauaugca gacccugaca     1620

ccugugggcc gccucauuac cgccaacccc gugaucacgg aguccacaga aaacagcaag     1680

augaugcugg agcuugaccc gccuuucggg gauucuuaca ucguuauugg cgucggagag     1740

aaaaagauca cccaccacug gcaucggucc ggcagcacaa uugggaaggc guuugaagcc     1800

accgugagag gagcuaaacg cauggccgug cugggcgaca ccgcuuggga uuucgggucc     1860

gucggaggcg cccucaauuc ucuggggaag ggcauccacc agauuuucgg agccgcuuuu     1920

aaaagccugu ucggcgggau guccugguuu ucucaaaucc uuaucggaac acuccugaug     1980

uggcugggcc ucaacacgaa gaaugggagc auuucccuga ugugucuugc gcugggaggc     2040

gugcucaucu uccugagcac cgccguuucu gcugac                               2076


<210>  2264
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2264
ggcgccgaca ccagcguggg gaucgucgga cugcuccuga caacggcuau ggccgcggag       60

guuaccagac ggggcuccgc uuacuauaug uaccuugauc gcaacgacgc cggggaagcc      120

auuucuuucc ccacaacccu gggaaugaau aagugcuaua uccagauuau ggaucucggc      180

cacaugugug acgcuacaau gagcuacgag ugcccuaugc uggaugaagg gguggagccg      240

gacgaugugg acuguuggug caacaccaca uccaccuggg ucguguaugg aacgugucau      300

cacaaaaagg gcgaagccag acggucucgc agagcuguua cccuucccag ccauuccaca      360

cggaaacugc aaacccgcuc ucagacaugg cucgagagca gagaguacac caagcaccug      420

auccgggugg aaaauuggau uuuucgcaac ccuggcuucg cccuggcggc cgcugccauc      480

gcuuggcucc ugggguccag cacgucucag aaagucaucu aucuugugau gauucugcuc      540

aucgcccccg cguacuccau uagaugcauc ggaguuagca aucgggauuu uguggagggc      600

augucugggg gaacaugggu cgacauugug cuggaacaug gcgggugugu gaccgucaug      660

gcccaggaua agccuacagu ugacaucgag cuugugacca ccacaguguc caacauggcu      720

gagguccgca gcuacugcua ugaagccucu auuuccgaua uggcuagcga cuccagaugu      780

cccacgcaag gagaggccua ccuggacaag cagucugaua cccaguaugu gugcaaacgg      840

acacucguug accgcggcug gggcaauggg uguggacugu ucggcaaggg gagccuggug      900

accugcgcca aauuugcuug cuccaagaaa augacaggaa agagcaucca gccugaaaac      960

cucgaguaca gaaucaugcu gucuguccac ggcucccaac acagcgggau gauugugaau     1020

gauaccggac augaaacgga cgagaaccgg gcgaagguug agaucacacc caauucuccc     1080

agagccgaag cuacccuugg cggcuucggg agccugggac ucgauuguga gccucgcacc     1140

ggccuggacu uuuccgaucu uuauuaccug acaaugaaca auaaacacug gcucgugcau     1200

aaggaauggu uccacgacau uccccugccu uggcacgccg gggccgauac cggaacaccc     1260

cauuggaaca auaaagaggc ucuggucgaa uuuaaggacg cccacgcgaa acggcagacg     1320

guggucgugc ucggcucuca ggagggggcu guucauaccg cccuggccgg cgcucuugag     1380

gccgaaaugg auggagcuaa gggcagacug agcuccgggc accucaaaug ccgccugaag     1440

auggacaaac uucggcugaa gggagugagc uauucucucu guacagccgc guucaccuuu     1500

acaaagaucc cggccgagac ccugcauggc acggucaccg uggaaguuca guacgcuggg     1560

acagacggac cuugcaaagu gcccgcccaa auggcugucg auaugcagac ccugacaccu     1620

gugggcagac ucauuaccgc caaccccgug aucacggagu ccacagaaaa cagcaagaug     1680

augcuggagc uugacccgcc uuucggggau ucuuacaucg uuauuggcgu cggagagaaa     1740

aagaucaccc accacuggca ucgcuccggc agcacaauug ggaaggcguu ugaagccacc     1800

gugcggggag cuaaaagaau ggccgugcug ggcgacaccg cuugggauuu cggguccguc     1860

ggaggcgccc ucaauucucu ggggaagggc auccaccaga uuuucggagc cgcuuuuaaa     1920

agccuguucg gcgggauguc cugguuuucu caaauccuua ucggaacacu ccugaugugg     1980

cugggccuca acacgaagaa ugggagcauu ucccugaugu gucuugcgcu gggaggcgug     2040

cucaucuucc ugagcaccgc cguuucugcu gac                                  2073


<210>  2265
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2265
gccgacacca gcgugggcau cgucgggcug cuccugacaa cggcuauggc cgcggagguu       60

accagacggg gauccgcuua cuauauguac cuugaucgca acgacgccgg cgaagccauu      120

ucuuucccca caacccuggg gaugaauaag ugcuauaucc agauuaugga ucucggacac      180

augugugacg cuacaaugag cuacgagugc ccuaugcugg augaaggcgu ggagccggac      240

gauguggacu guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac      300

aaaaagggag aagccagacg gucucgcaga gcuguuaccc uucccagcca uuccacacgg      360

aaacugcaaa cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc      420

cggguggaaa auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu      480

uggcuccugg gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc      540

gcccccgcgu acuccauuag augcaucggg guuagcaauc gggauuuugu ggagggaaug      600

ucuggcggga caugggucga cauugugcug gaacauggag gcugugugac cgucauggcc      660

caggauaagc cuacaguuga caucgagcuu gugaccacca caguguccaa cauggcugag      720

guccgcagcu acugcuauga agccucuauu uccgauaugg cuagcgacuc cagauguccc      780

acgcaagggg aggccuaccu ggacaagcag ucugauaccc aguaugugug caaacggaca      840

cucguugacc gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc      900

ugcgccaaau uugcuugcuc caagaaaaug acaggaaaga gcauccagcc ugaaaaccuc      960

gaguacagaa ucaugcuguc uguccacggg ucccaacaca gcggcaugau ugugaaugau     1020

accgggcaug aaacggacga gaaccgggcg aagguugaga ucacacccaa uucucccaga     1080

gccgaagcua cccuuggagg cuucggcagc cuggggcucg auugugagcc ucgcaccgga     1140

cuggacuuuu ccgaucuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag     1200

gaaugguucc acgacauucc ccugccuugg cacgccggcg ccgauaccgg gacaccccau     1260

uggaacaaua aagaggcucu ggucgaauuu aaggacgccc acgcgaaacg gcagacggug     1320

gucgugcucg gaucucagga gggcgcuguu cauaccgccc uggccggggc ucuugaggcc     1380

gaaauggaug gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug     1440

gacaagcugc ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca     1500

aagaucccgg ccgagacccu gcauggaacg gucaccgugg aaguucagua cgcuggcaca     1560

gacgggccuu gcaaagugcc cgcccaaaug gcugucgaua ugcagacccu gacaccugug     1620

ggaagacuca uuaccgccaa ccccgugauc acggagucca cagaaaacag caagaugaug     1680

cuggagcuug acccgccuuu cggcgauucu uacaucguua uuggggucgg cgagaaaaag     1740

aucacccacc acuggcaucg cuccggaagc acaauuggca aggcguuuga agccaccgug     1800

agaggggcua aacggauggc cgugcuggga gacaccgcuu gggauuucgg cuccgucggg     1860

ggagcccuca auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc     1920

cuguucggag gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug     1980

ggacucaaca cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc     2040

aucuuccuga gcaccgccgu uucugcugac                                      2070


<210>  2266
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2266
gacaccagcg ugggcaucgu cgggcugcuc cugacaacgg ccauggcugc cgagguuacc       60

agacggggau ccgcguacua uauguaccuu gaucgcaacg acgcuggcga agccauuucu      120

uuccccacaa cccuggggau gaauaagugc uauauccaga uuauggaucu cggacacaug      180

ugugacgcca caaugagcua cgagugcccu augcuggaug aaggcgugga gccggacgau      240

guggacuguu ggugcaacac cacauccacc ugggucgugu augggacgug ucaucacaaa      300

aagggagaag cuagacgguc ucgcagagcc guuacccuuc ccagccauuc cacacggaaa      360

cugcaaaccc gcucucagac auggcucgag agcagagagu acaccaagca ccugauccgg      420

guggaaaauu ggauuuuucg caacccuggc uucgcucugg ccgcggccgc uaucgccugg      480

cuccugggcu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcu      540

cccgccuacu ccauuagaug caucgggguu agcaaucggg auuuugugga gggaaugucu      600

ggcgggacau gggucgacau ugugcuggaa cauggaggcu gugugaccgu cauggcgcag      660

gauaagccua caguugacau cgagcuugug accaccacag uguccaacau ggccgagguc      720

cgcagcuacu gcuaugaagc uagcauuucc gauauggcca gcgacuccag augucccacg      780

caaggggagg cuuaccugga caagcagucu gauacccagu augugugcaa acggacacuc      840

guugaccgcg gauggggcaa uggcuguggg cuguucggaa agggcagccu ggugaccugc      900

gccaaauuug ccugcuccaa gaaaaugaca gggaagucua uccagccuga aaaccucgag      960

uacagaauca ugcugucugu ccacggaucc caacacagcg gcaugauugu gaaugauacc     1020

gggcaugaaa cggacgagaa ccgggcuaag guugagauca cacccaauuc ucccagagcg     1080

gaagccaccc uuggaggcuu cggcagccug gggcucgauu gugagccucg caccggacug     1140

gacuuuuccg aucuuuauua ccugacaaug aacaauaaac acuggcucgu gcauaaggaa     1200

ugguuccacg acauuccccu gccuuggcac gcuggcgccg auaccgggac accccauugg     1260

aacaauaaag aggcccuggu cgaauuuaag gacgcucacg ccaaacggca gacggugguc     1320

gugcucggau cucaggaggg cgcgguucau accgcucugg ccggggcccu ugaggcugaa     1380

auggauggcg ccaagggaag acugagcucc ggccaccuca aaugccgccu gaagauggac     1440

aagcugcggc ugaaaggggu gagcuauucu cucuguacag cugccuucac cuuuacaaag     1500

aucccggcgg agacccugca uggaacgguc accguggaag uucaguacgc cggcacagac     1560

gggccuugca aagugcccgc ucaaauggcc gucgauaugc agacccugac accuguggga     1620

agacucauua ccgcuaaccc cgugaucacg gaguccacag aaaacagcaa gaugaugcug     1680

gagcuugacc cgccuuucgg cgauucuuac aucguuauug gggucggcga gaaaaagauc     1740

acccaccacu ggcaucgcuc cggaagcaca auuggcaagg ccuuugaagc gaccgugaga     1800

ggggccaaac ggauggcugu gcugggagac accgccuggg auuucggcuc cgucggggga     1860

gcucucaauu cucugggcaa ggggauccac cagauuuucg gcgccgccuu uaaaagccug     1920

uucggaggca uguccugguu uucucaaauc cuuaucggga cacuccugau guggcuggga     1980

cucaacacga agaauggcag cauuucccug augugucuug cucuuggggg agugcucauc     2040

uuccugagca ccgcgguuuc ugccgac                                         2067


<210>  2267
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2267
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc cgau                                            2064


<210>  2268
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2268
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccgc gguuaccaga       60

cggggauccg cuuacuauau guaccuugac cgcaacgaug ccggcgaggc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugcuacaa ugagcuacga augcccuaug cuggacgagg gcguggaacc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaggcca gacggucucg cagagcuguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgaaagc agagaguaca ccaagcaccu gauccgggug      420

gagaauugga uuuuucgcaa cccuggcuuc gcccuggcgg ccgcugccau cgcuuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgccccc      540

gcguacucca uuagaugcau cgggguuagc aaucgggacu uuguggaagg aaugucuggc      600

gggacauggg ucgauauugu gcuggagcau ggaggcugug ugaccgucau ggcccaggac      660

aagccuacag uugauaucga acuugugacc accacagugu ccaacauggc ugagguccgc      720

agcuacugcu augaggccuc uauuuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaagccu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugcuu gcuccaagaa aaugacaggg aagucuaucc agccugagaa ccucgaauac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugagacgg augaaaaccg ggcgaagguu gagaucacac cgaauagccc cagagccgag     1080

gcuacccuug gaggcuucgg cagccugggg cucgacugug aaccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggagugg     1200

uuccacgaua uuccccugcc uuggcacgcc ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagaag cucuggucga guuuaaggau gcccacgcga aacggcagac gguggucgug     1320

cucggaucuc aggaaggcgc uguucauacc gcccuggccg gggcucuuga ggccgagaug     1380

gacggcgcua agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagccg cguucaccuu uacaaagauc     1500

ccggccgaaa cccugcaugg aacggucacc guggagguuc aguacgcugg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcuguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg ccaaccccgu gaucacggaa uccacagaga acagcaagau gaugcuggaa     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggcgu uugaggccac cgugagaggg     1800

gcuaaacgga uggccgugcu gggagauacc gcuugggacu ucggcuccgu cgggggagcc     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcgc uugggggagu gcucaucuuc     2040

cugagcaccg ccguuucugc ugau                                            2064


<210>  2269
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2269
agaggcgccg acaccagcgu ggggaucguc ggacugcucc ugacaacggc uauggccgcg       60

gcuguuaccc ggcgcggcuc cgccuacuau auguaccuug auagaaacga cgccggggag      120

gcuauuucuu uccccacaac ccugggaaug aauaagugcu auauccagau uauggaucuc      180

ggccacaugu gugacgccac aaugagcuac gaaugcccua ugcuggauga ggggguggaa      240

ccggacgaug uggacuguug gugcaacacc acauccaccu gggucgugua uggaacgugu      300

caucacaaaa agggcgaggc ucggcgcucu agacgggccg uuacccuucc cagccauucc      360

acacgcaaac ugcaaaccag aucucagaca uggcucgaaa gccgggagua caccaagcac      420

cugauccgcg uggagaauug gauuuuuaga aacccuggcu ucgcgcuggc cgcugccgcu      480

aucgccuggc uccugggguc cagcacgucu cagaaaguca ucuaucuugu gaugauucug      540

cucaucgcgc ccgccuacuc cauucggugc aucggaguua gcaaucgcga uuuuguggaa      600

ggcaugucug ggggaacaug ggucgacauu gugcuggagc auggcgggug ugugaccguc      660

auggcucagg auaagccuac aguugacauc gaacuuguga ccaccacagu guccaacaug      720

gccgagguca gaagcuacug cuaugaggcu ucuauuuccg auauggccag cgacucccgg      780

ugucccacgc aaggagaagc cuaccuggac aagcagucug auacccagua ugugugcaaa      840

cgcacacucg uugacagagg cuggggcaau ggguguggac uguucggcaa ggggagccug      900

gugaccugcg cuaaauuugc gugcuccaag aaaaugacag gaaagagcau ccagccugag      960

aaccucgaau accggaucau gcugucuguc cacggcuccc aacacagcgg gaugauugug     1020

aaugauaccg gacaugagac ggacgaaaac agagccaagg uugagaucac acccaauucu     1080

ccccgcgcug aggccacccu uggcggcuuc gggagccugg gacucgauug ugaaccucgg     1140

accggccugg acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug     1200

cauaaggagu gguuccacga cauuccccug ccuuggcacg ccggggcuga uaccggaaca     1260

ccccauugga acaauaaaga agcccugguc gaguuuaagg acgcgcacgc uaaaagacag     1320

acgguggucg ugcucggcuc ucaggaaggg gccguucaua ccgcccuggc uggcgcccuu     1380

gaggcugaga uggauggagc caagggccgc cugagcuccg ggcaccucaa augccggcug     1440

aagauggaca aacuuagacu gaagggagug agcuauucuc ucuguacagc ggccuucacc     1500

uuuacaaaga ucccggcuga aacccugcau ggcacgguca ccguggaggu ucaguacgcc     1560

gggacagacg gaccuugcaa agugcccgcu caaauggccg ucgauaugca gacccugaca     1620

ccugugggcc gccucauuac cgcgaacccc gugaucacgg aauccacaga gaacagcaag     1680

augaugcugg aacuugaccc gccuuucggg gauucuuaca ucguuauugg cgucggagag     1740

aaaaagauca cccaccacug gcaucggucc ggcagcacaa uugggaaggc cuuugaggcu     1800

accgugagag gagccaaacg cauggcugug cugggcgaca ccgccuggga uuucgggucc     1860

gucggaggcg cccucaauuc ucuggggaag ggcauccacc agauuuucgg agcugcguuu     1920

aaaagccugu ucggcgggau guccugguuu ucucaaaucc uuaucggaac acuccugaug     1980

uggcugggcc ucaacacgaa gaaugggagc auuucccuga ugugucuugc ccugggaggc     2040

gugcucaucu uccugagcac cgcuguuucu gccgac                               2076


<210>  2270
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2270
agaggccuga gcucccggaa gcgcagaucu cacgacgugc ucaccgucca guuccugauc       60

cuugggaugc ugcucaugac aggaggcguu acgcggcgcg ggagcgccua cuauauguac      120

cuggauagaa acgacgcugg agaggccauu uccuuuccca ccacacuugg caugaauaaa      180

ugcuauaucc aaauuaugga ucuggggcau augugugacg cgaccauguc uuacgaaugc      240

ccuaugcucg augagggagu ggaaccggac gauguggacu guuggugcaa cacaaccagc      300

acaugggucg uguauggcac cugucaccau aagaaaggcg aggcucggcg cuccagacgg      360

gccguuacgc ugcccucuca cagcacccgc aagcugcaga caagauccca gaccuggcuc      420

gaaagccggg aguacacaaa acaucugauc cgcguggaga auuggauuuu cagaaacccu      480

ggguuugccc uugcugccgc ugccaucgcg uggcugcucg gaucuuccac cagccagaag      540

gucaucuauc uggugaugau ucuucugauc gcccccgcuu acucuauucg gugcaucggc      600

guuuccaauc gcgauuucgu ggaagggaug agcggaggca cgugggucga cauugugcuc      660

gagcacgggg gaugugugac agucauggcc caagauaagc cuaccguuga caucgaacug      720

gugacaacca ccgugucuaa cauggcugag gucagauccu acugcuauga ggccagcauu      780

uccgauaugg cgucugacag ccgguguccc acacagggcg aagccuaccu ggacaaacag      840

uccgauacgc aguaugugug caagagaacc cucguugacc gcggcugggg gaauggaugu      900

ggccuguuug ggaaaggaag ccuugugaca ugcgcuaagu ucgccugcuc uaaaaagaug      960

accggcaagu ccauccaacc ugagaaccug gaauaccgga ucaugcucag cguccacggg     1020

ucucagcaua gcggaaugau ugugaaugau acaggccacg agaccgacga aaacagagcu     1080

aaaguugaga ucacgcccaa uuccccgcgc gccgaggcca cacugggcgg guuuggaucu     1140

cuuggccugg auugugaacc ucggaccggg cucgacuuca gcgaucugua uuaccugacc     1200

augaacaaua agcauuggcu cgugcacaaa gagugguuuc acgacauucc ccugccuugg     1260

caugcuggag cggauacagg cacaccccac uggaacaaua aggaagcccu ugucgaguuc     1320

aaagacgcuc augccaagag acagacagug gucgugcugg ggucccagga aggcgccguu     1380

cacacggcuc ucgccggagc gcuggaggcu gagauggaug gcgccaaagg gcgccuuagc     1440

ucuggacauc ugaagugccg gcucaagaug gacaaacuga gacugaaggg cguguccuau     1500

agccucugua ccgccgcuuu uacauucacc aaaaucccgg ccgaaacacu gcacgggacc     1560

gucacggugg agguucaaua cgcuggaacc gacggcccuu gcaaggugcc cgcccagaug     1620

gcggucgaua ugcagacacu uaccccugug gggcgccuga uuacagccaa ccccgugauc     1680

accgaaucua cggagaacuc caaaaugaug cucgaacugg aucccccuuu uggcgacagc     1740

uacaucguua uuggagucgg cgagaagaag aucacacacc auuggcaccg guccgggucu     1800

accauuggaa aggcuuucga ggccacagug agaggcgcua aacgcauggc cgugcugggg     1860

gacaccgcgu gggauuucgg aagcgucggc ggggcccuua auucccucgg caaaggaauc     1920

caucaaauuu uuggcgcugc cuucaagucu cuguuugggg gaaugagcug guucucccag     1980

auccugaucg gcacccuccu gauguggcuu gggcugaaca ccaaaaaugg aagcauuucu     2040

cucauguguc uggcucuggg cggggugcuu aucuuucucu ccacggccgu uagcgccgac     2100


<210>  2271
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2271
agaggccuga gcucccggaa gcgcagaucu cacgacgugc ucaccgucca guuccugauc       60

cuugggaugc ugcucaugac aggagccguu acgcggcgcg gcagcgcuua cuauauguac      120

cuggauagaa acgacgccgg ggaggcgauu uccuuuccca ccacacuugg aaugaauaaa      180

ugcuauaucc aaauuaugga ucugggccau augugugacg cuaccauguc uuacgaaugc      240

ccuaugcucg augagggggu ggaaccggac gauguggacu guuggugcaa cacaaccagc      300

acaugggucg uguauggaac cugucaccau aagaaaggcg aggcccggcg cuccagacgg      360

gccguuacgc ugcccucuca cagcacccgc aagcugcaga caagauccca gaccuggcuc      420

gaaagccggg aguacacaaa acaucugauc cgcguggaga auuggauuuu cagaaacccu      480

ggcuuugcuc uugccgcugc cgcgaucgcc uggcugcucg ggucuuccac cagccagaag      540

gucaucuauc uggugaugau ucuucugauc gcucccgccu acucuauucg gugcaucgga      600

guuuccaauc gcgauuucgu ggaaggcaug agcgggggaa cgugggucga cauugugcuc      660

gagcacggcg ggugugugac agucauggcu caagauaagc cuaccguuga caucgaacug      720

gugacaacca ccgugucuaa cauggccgag gucagauccu acugcuauga ggcgagcauu      780

uccgauaugg ccucugacag ccgguguccc acacagggag aggcuuaccu ggacaaacag      840

uccgauacgc aguaugugug caagagaacc cucguugacc gcggcugggg caaugggugu      900

ggacuguuug gcaaagggag ccuugugaca ugcgccaagu ucgcuugcuc uaaaaagaug      960

accggaaagu ccauccaacc ugaaaaccug gaauaccgga ucaugcucag cguccacggc     1020

ucucagcaua gcgggaugau ugugaaugau acaggacacg agaccgacga aaacagagcc     1080

aaaguugaga ucacgcccaa uuccccgcgc gccgaggcua cacugggcgg cuuugggucu     1140

cuuggacugg auugugaacc ucggaccggc cucgacuuca gcgaucugua uuaccugacc     1200

augaacaaua agcauuggcu cgugcacaaa gagugguuuc acgacauucc ccugccuugg     1260

caugcggggg ccgauacagg aacaccccac uggaacaaua aggaagcucu ugucgaguuc     1320

aaagacgccc augccaagag acagacagug gucgugcugg gcucccagga aggggcuguu     1380

cacacggccc ucgcgggcgc ucuggaggcc gagauggaug gagccaaagg ccgccuuagc     1440

ucugggcauc ugaagugccg gcucaagaug gacaaacuga gacugaaggg aguguccuau     1500

agccucugua ccgcugccuu uacauucacc aaaaucccgg cugaaacacu gcacggcacc     1560

gucacggugg agguucaaua cgccgggacc gacggaccuu gcaaggugcc cgcgcagaug     1620

gccgucgaua ugcagacacu uaccccugug ggccgccuga uuacagcuaa ccccgugauc     1680

accgaaucua cggagaacuc caaaaugaug cucgaacugg aucccccuuu uggggacagc     1740

uacaucguua uuggcgucgg agagaagaag aucacacacc auuggcaccg guccggcucu     1800

accauuggga aagccuucga ggcuacagug agaggagcca agcgcauggc ggugcugggc     1860

gacaccgccu gggauuucgg gagcgucgga ggcgcucuua auucccucgg gaaaggcauc     1920

caucaaauuu uuggagccgc uuucaagucu cuguuuggcg ggaugagcug guucucccag     1980

auccugaucg gaacccuccu gauguggcuu ggccugaaca ccaaaaaugg gagcauuucu     2040

cucauguguc uggcccuggg aggcgugcuu aucuuucucu ccacggccgu uagcgcugac     2100


<210>  2272
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2272
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaau auggccgaag ucagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguugaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcgca cgcuaagaga cagacggugg ucgugcucgg cucucaggag     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccgugauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcgugcuca ucuuccugag caccgcuguu     2100

ucugcc                                                                2106


<210>  2273
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2273
aucaacgcca gaaaggagaa aaagcggcgc ggcgcugaca ccagcguggg gauugucgga       60

cugcuccuga caacggccau ggcggcugaa guuaccagac ggggcuccgc cuacuauaug      120

uaccuugauc gcaaugacgc cggggaggcu aucucuuucc ccacaacccu gggaaugaac      180

aaaugcuaua uucagaucau ggaucucggc cacaugugug acgccacaau gagcuacgaa      240

ugcccuaugc uggaugaggg gguggaaccg gacgaugugg acuguuggug caauaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaagaaag gcgaggcuag acggucucgc      360

agagccguua cccuucccag ccauuccaca cggaagcugc aaacccgcuc ucagacaugg      420

cucgagagca gagaauacac caagcaccug auucgggugg agaacuggau cuuucgcaau      480

ccuggcuucg cgcuggccgc ugccgcuauc gccuggcucc ugggguccag cacgucucag      540

aaagucauuu aucuugugau gauccugcuc auugcgcccg ccuacuccau cagaugcauu      600

ggaguuagca accgggauuu uguggaaggc augucugggg gaacaugggu cgacaucgug      660

cuggagcaug gcgggugugu gaccgucaug gcucaggaua agccuacagu ugacauugag      720

cuugugacca ccacaguguc caauauggcc gaaguccgca gcuacugcua ugaggcuucu      780

aucuccgaua uggccagcga cuccagaugu cccacgcaag gagaagccua ccuggacaaa      840

cagucugaua cccaguaugu gugcaagcgg acacucguug acagaggcug gggcaacggg      900

uguggacugu ucggcaaagg gagccuggug accugcgcua aguuugcgug cuccaagaaa      960

augacaggaa agagcaucca gccugagaau cucgaauacc gcauuaugcu gucuguccac     1020

ggcucccaac acagcgggau gaucgugaac gauaccggac augagacgga cgagaaucgg     1080

gccaaaguug aaauuacacc gaacucuccc agagcugagg ccacccuugg cggcuucggg     1140

agccugggac ucgauuguga accucgcacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaua acaagcacug gcucgugcau aaagaguggu uccacgacau cccccugccu     1260

uggcacgccg gggcugauac cggaacaccc cauuggaaua acaaggaagc ccuggucgag     1320

uuuaaagacg cgcacgcuaa gcggcagacg guggucgugc ucggcucuca ggagggggcc     1380

guucauaccg cccuggcugg cgcccuugaa gcugagaugg auggagccaa gggcagacug     1440

agcuccgggc accucaaaug ccgccugaag auggacaaac uucggcugaa gggagugagc     1500

uauucucucu guacagcggc cuucaccuuu acaaaaauuc cggcugaaac ccugcauggc     1560

acggucaccg uggagguuca guacgccggg acagacggac cuugcaaggu gcccgcucaa     1620

auggccgucg auaugcagac ccugacaccu gugggcagac ucaucaccgc gaaccccgug     1680

aucacggaau ccacagagaa uagcaagaug augcuggagc uugauccccc uuucggggac     1740

ucuuacauug uuaucggcgu cggagagaaa aagauuaccc accacuggca ucgcuccggc     1800

agcacaaucg ggaaagccuu ugaagcuacc gugcggggag ccaagagaau ggcugugcug     1860

ggcgacaccg ccugggauuu cggguccguc ggaggcgccc ucaacucucu ggggaaaggc     1920

auucaccaga ucuucggagc ugcguuuaag agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua uuggaacacu ccugaugugg cugggccuca auacgaagaa cgggagcauc     2040

ucccugaugu gucuugcccu gggaggcgug cucauuuucc ugagcaccgc uguuucugcc     2100


<210>  2274
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2274
aucauuaacg ccagaaagga gaaaaagcgg cgcggcgcug acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc cauggcggcu gaaguuacca gacggggcuc cgccuacuau      120

auguaccuug aucgcaauga cgccggggag gcuauuucuu uccccacaac ccugggaaug      180

aacaaaugcu auauccagau uauggaucuc ggccacaugu gugacgccac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaauacc      300

acauccaccu gggucgugua uggaacgugu caucacaaga aaggcgaggc uagacggucu      360

cgcagagccg uuacccuucc cagccauucc acacggaagc ugcaaacccg cucucagaca      420

uggcucgaga gcagagaaua caccaagcac cugauccggg uggagaacug gaucuuucgc      480

aauccuggcu ucgcgcuggc cgcugccgcu auugccuggc uccugggguc cagcacgucu      540

cagaaaguca ucuaucuugu gaugauucug cucaucgcgc ccgccuacuc cauuagaugc      600

aucggaguua gcaaccggga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcucagg auaagccuac aguugacauc      720

gagcuuguga ccaccacagu guccaauaug gccgaagucc gcagcuacug cuaugaggcu      780

ucuaucuccg auauggccag cgacuccaga ugucccacgc aaggagaagc cuaccuggac      840

aaacagucug auacccagua ugugugcaag cggacacucg uugacagagg cuggggcaac      900

ggguguggac uguucggcaa agggagccug gugaccugcg cuaaguuugc gugcuccaag      960

aaaaugacag gaaagagcau ucagccugag aaucucgaau accgcaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aacgauaccg gacaugagac ggacgagaau     1080

cgggccaaag uugaaaucac accgaacucu cccagagcug aggccacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccucgc accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga auaacaagca cuggcucgug cauaaagagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggcuga uaccggaaca ccccauugga auaacaagga agcccugguc     1320

gaguuuaaag acgcgcacgc uaagcggcag acgguggucg ugcucggcuc ucaggagggg     1380

gccguucaua ccgcccuggc uggcgcccuu gaagcugaga uggauggagc caagggcaga     1440

cugagcuccg ggcaccucaa augccgccug aagauggaca aacuucggcu gaagggagug     1500

agcuauucuc ucuguacagc ggccuucacc uuuacaaaaa ucccggcuga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcc gggacagacg gaccuugcaa ggugcccgcu     1620

caaauggccg ucgauaugca gacccugaca ccugugggca gacucaucac cgcgaacccc     1680

gugauuacgg aauccacaga gaauagcaag augaugcugg agcuugaucc cccuuucggg     1740

gacucuuaca ucguuauugg cgucggagag aaaaagauca cccaccacug gcaucgcucc     1800

ggcagcacaa uugggaaagc cuuugaagcu accgugcggg gagccaagag aauggcugug     1860

cugggcgaca ccgccuggga uuucgggucc gucggaggcg cccucaacuc ucuggggaaa     1920

ggcauccacc agaucuucgg agcugcguuu aagagccugu ucggcgggau guccugguuu     1980

ucucaaauuc uuaucggaac acuccugaug uggcugggcc ucaauacgaa gaacgggagc     2040

auuucccuga ugugucuugc ccugggaggc gugcucaucu uccugagcac cgcuguuucu     2100

gcc                                                                   2103


<210>  2275
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2275
aacgccagaa aggagaaaaa gcggcgcggc gcugacacca gcguggggau cgucggacug       60

cuccugacaa cggccauggc ggcugaaguu accagacggg gcuccgccua cuauauguac      120

cuugaucgca augacgccgg ggaggcuauu ucuuucccca caacccuggg aaugaacaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg ccacaaugag cuacgaaugc      240

ccuaugcugg augagggggu ggaaccggac gauguggacu guuggugcaa uaccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aggcuagacg gucucgcaga      360

gccguuaccc uucccagcca uuccacacgg aagcugcaaa cccgcucuca gacauggcuc      420

gagagcagag aauacaccaa gcaccugauc cggguggaga acuggauuuu ucgcaauccu      480

ggcuucgcgc uggccgcugc cgcuaucgcc uggcuccugg gguccagcac gucucagaaa      540

gucaucuauc uugugaugau ucugcucauc gcgcccgccu acuccauuag augcaucgga      600

guuagcaacc gggauuuugu ggaaggcaug ucugggggaa caugggucga cauugugcug      660

gagcauggcg ggugugugac cgucauggcu caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa uauggccgaa guccgcagcu acugcuauga ggcuucuauu      780

uccgauaugg ccagcgacuc cagauguccc acgcaaggag aagccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagcggaca cucguugaca gaggcugggg caacgggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgcuaagu uugcgugcuc caagaaaaug      960

acaggaaaga gcauccagcc ugagaaucuc gaauaccgca ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaacgau accggacaug agacggacga gaaucgggcc     1080

aaaguugaaa ucacaccgaa cucucccaga gcugaggcca cccuuggcgg cuucgggagc     1140

cugggacucg auugugaacc ucgcaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaauaaca agcacuggcu cgugcauaaa gagugguucc acgacauucc ccugccuugg     1260

cacgccgggg cugauaccgg aacaccccau uggaauaaca aggaagcccu ggucgaguuu     1320

aaagacgcgc acgcuaagcg gcagacggug gucgugcucg gcucucagga gggggccguu     1380

cauaccgccc uggcuggcgc ccuugaagcu gagauggaug gagccaaggg cagacugagc     1440

uccgggcacc ucaaaugccg ccugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucucucugua cagcggccuu caccuuuaca aaaaucccgg cugaaacccu gcauggcacg     1560

gucaccgugg agguucagua cgccgggaca gacggaccuu gcaaggugcc cgcucaaaug     1620

gccgucgaua ugcagacccu gacaccugug ggcagacuca uuaccgcgaa ccccgugauc     1680

acggaaucca cagagaauag caagaugaug cuggagcuug aucccccuuu cggggacucu     1740

uacaucguua uuggcgucgg agagaaaaag aucacccacc acuggcaucg cuccggcagc     1800

acaauuggga aagccuuuga agcuaccgug cggggagcca agagaauggc ugugcugggc     1860

gacaccgccu gggauuucgg guccgucgga ggcgcccuca acucucuggg gaaaggcauc     1920

caccagauuu ucggagcugc guuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaua cgaagaacgg gagcauuucc     2040

cugauguguc uugcccuggg aggcgugcuc aucuuccuga gcaccgcugu uucugcc        2097


<210>  2276
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2276
gccagaaagg agaaaaagcg gcgcggcgcu gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg ccauggcggc ugaaguuacc agacggggcu ccgccuacua uauguaccuu      120

gaucgcaacg acgccgggga ggcuauuucu uuccccacaa cccugggaau gaauaaaugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcca caaugagcua cgaaugcccu      240

augcuggaug agggggugga accggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaag aaaggcgagg cuagacgguc ucgcagagcc      360

guuacccuuc ccagccauuc cacacggaag cugcaaaccc gcucucagac auggcucgag      420

agcagagaau acaccaagca ccugauccgg guggagaauu ggauuuuucg caacccuggc      480

uucgcgcugg ccgcugccgc uaucgccugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcg cccgccuacu ccauuagaug caucggaguu      600

agcaaucggg auuuugugga aggcaugucu gggggaacau gggucgacau ugugcuggag      660

cauggcgggu gugugaccgu cauggcucag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggccgaaguc cgcagcuacu gcuaugaggc uucuauuucc      780

gauauggcca gcgacuccag augucccacg caaggagaag ccuaccugga caaacagucu      840

gauacccagu augugugcaa gcggacacuc guugacagag gcuggggcaa ugggugugga      900

cuguucggca aagggagccu ggugaccugc gcuaaguuug cgugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga gaaccucgaa uaccgcauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaga cggacgagaa ccgggccaaa     1080

guugaaauca cacccaauuc ucccagagcu gaggccaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugaaccucg caccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaagc acuggcucgu gcauaaagag ugguuccacg acauuccccu gccuuggcac     1260

gccggggcug auaccggaac accccauugg aacaauaagg aagcccuggu cgaguuuaaa     1320

gacgcgcacg cuaagcggca gacggugguc gugcucggcu cucaggaggg ggccguucau     1380

accgcccugg cuggcgcccu ugaagcugag auggauggag ccaagggcag acugagcucc     1440

gggcaccuca aaugccgccu gaagauggac aaacuucggc ugaagggagu gagcuauucu     1500

cucuguacag cggccuucac cuuuacaaaa aucccggcug aaacccugca uggcacgguc     1560

accguggagg uucaguacgc cgggacagac ggaccuugca aggugcccgc ucaaauggcc     1620

gucgauaugc agacccugac accugugggc agacucauua ccgcgaaccc cgugaucacg     1680

gaauccacag agaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcaucgcuc cggcagcaca     1800

auugggaaag ccuuugaagc uaccgugcgg ggagccaaga gaauggcugu gcugggcgac     1860

accgccuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa aggcauccac     1920

cagauuuucg gagcugcguu uaagagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cccugggagg cgugcucauc uuccugagca ccgcuguuuc ugcc           2094


<210>  2277
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2277
agaaaggaga aaaagcggcg cggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga aguuaccaga cggggcuccg cuuacuauau guaccuugau      120

cgcaacgacg ccggggaggc cauuucuuuc cccacaaccc ugggaaugaa uaaaugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaagaaa ggcgaggcca gacggucucg cagagcuguu      360

acccuuccca gccauuccac acggaagcug caaacccgcu cucagacaug gcucgagagc      420

agagaauaca ccaagcaccu gauccgggug gagaauugga uuuuucgcaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uuagaugcau cggaguuagc      600

aaucgggauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaaguccgc agcuacugcu augaggccuc uauuuccgau      780

auggcuagcg acuccagaug ucccacgcaa ggagaagccu accuggacaa acagucugau      840

acccaguaug ugugcaagcg gacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaaag ggagccuggu gaccugcgcc aaguuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cgcaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgagaaccg ggcgaaaguu     1080

gaaaucacac ccaauucucc cagagccgag gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucgcac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaagcacu ggcucgugca uaaagagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaggaag cucuggucga guuuaaagac     1320

gcccacgcga agcggcagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga agccgagaug gauggagcua agggcagacu gagcuccggg     1440

caccucaaau gccgccugaa gauggacaaa cuucggcuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaaaauc ccggccgaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgcugg gacagacgga ccuugcaagg ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggcaga cucauuaccg ccaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgcuccgg cagcacaauu     1800

gggaaagcgu uugaagccac cgugcgggga gcuaagagaa uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaagg cauccaccag     1920

auuuucggag ccgcuuuuaa gagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc u              2091


<210>  2278
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2278
aaggagaaaa agagacgggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaagu uacccgcaga ggcuccgcuu acuauaugua ccuugaucgg      120

aacgacgccg gggaggccau uucuuucccc acaacccugg gaaugaauaa augcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgaaug cccuaugcug      240

gaugaggggg uggaaccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caagaaaggc gaggcccgca gaucucggcg cgcuguuacc      360

cuucccagcc auuccacaag aaagcugcaa acccggucuc agacauggcu cgagagccgc      420

gaauacacca agcaccugau cagaguggag aauuggauuu uucggaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauuc gcugcaucgg aguuagcaau      600

agagauuuug uggaaggcau gucuggggga acaugggucg acauugugcu ggagcauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga aguccggagc uacugcuaug aggccucuau uuccgauaug      780

gcuagcgacu cccgcugucc cacgcaagga gaagccuacc uggacaaaca gucugauacc      840

caguaugugu gcaagagaac acucguugac cggggcuggg gcaaugggug uggacuguuc      900

ggcaaaggga gccuggugac cugcgccaag uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugagaaccu cgaauacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gagacggacg agaaccgcgc gaaaguugaa     1080

aucacaccca auucuccccg ggccgaggcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugaac cuagaaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aagcacuggc ucgugcauaa agagugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaggaagcuc uggucgaguu uaaagacgcc     1320

cacgcgaagc gccagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaagc cgagauggau ggagcuaagg gccggcugag cuccgggcac     1440

cucaaaugca gacugaagau ggacaaacuu cgccugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaaaaucccg gccgaaaccc ugcauggcac ggucaccgug     1560

gagguucagu acgcugggac agacggaccu ugcaaggugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggccggcuc auuaccgcca accccgugau cacggaaucc     1680

acagagaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcaua gauccggcag cacaauuggg     1800

aaagcguuug aagccaccgu gcgcggagcu aagcggaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaaaggcau ccaccagauu     1920

uucggagccg cuuuuaagag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcu                  2088


<210>  2279
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2279
gagaagaaaa gacggggcgc cgacaccagc guggggaucg ucggacugcu ccugacaacg       60

gcuauggccg cggaaguuac ccgcagaggc uccgcuuacu auauguaccu ugaucggaac      120

gacgccgggg aggccauuuc uuuccccaca acccugggaa ugaauaagug cuauauccag      180

auuauggauc ucggccacau gugugacgcu acaaugagcu acgaaugccc uaugcuggau      240

gagggggugg aaccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uauggaacgu gucaucacaa aaagggcgag gcccgcagau cucggcgcgc uguuacccuu      360

cccagccauu ccacaagaaa acugcaaacc cggucucaga cauggcucga gagccgcgaa      420

uacaccaagc accugaucag aguggagaau uggauuuuuc ggaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggg uccagcacgu cucagaaggu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauucgcu gcaucggagu uagcaauaga      600

gauuuugugg aaggcauguc ugggggaaca ugggucgaca uugugcugga gcauggcggg      660

ugugugaccg ucauggccca ggauaaaccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaagu ccggagcuac ugcuaugagg ccucuauuuc cgauauggcu      780

agcgacuccc gcugucccac gcaaggagaa gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aaagaacacu cguugaccgg ggcuggggca augggugugg acuguucggc      900

aaggggagcc uggugaccug cgccaaauuu gcuugcucca agaagaugac aggaaaaagc      960

auccagccug agaaccucga auacagaauc augcugucug uccacggcuc ccaacacagc     1020

gggaugauug ugaaugauac cggacaugag acggacgaga accgcgcgaa gguugaaauc     1080

acacccaauu cuccccgggc cgaggcuacc cuuggcggcu ucgggagccu gggacucgau     1140

ugugaaccua gaaccggccu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga gugguuccac gacauucccc ugccuuggca cgccggggcc     1260

gauaccggaa caccccauug gaacaauaaa gaagcucugg ucgaguuuaa ggacgcccac     1320

gcgaaacgcc agacgguggu cgugcucggc ucucaggagg gggcuguuca uaccgcccug     1380

gccggcgcuc uugaagccga gauggaugga gcuaagggcc ggcugagcuc cgggcaccuc     1440

aagugcagac ugaaaaugga caaacuucgc cugaagggag ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gaaacccugc auggcacggu caccguggag     1560

guucaguacg cugggacaga cggaccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg ccggcucauu accgccaacc ccgugaucac ggaauccaca     1680

gagaacagca agaugaugcu ggagcuugac ccgccuuucg gggauucuua caucguuauu     1740

ggcgucggag aaaagaaaau cacccaccac uggcauagau ccggcagcac aauugggaag     1800

gcguuugagg ccaccgugcg cggagcuaaa cggauggccg ugcugggcga caccgcuugg     1860

gauuucgggu ccgucggagg cgcccucaau ucucugggga agggcaucca ccagauuuuc     1920

ggagccgcuu uuaaaagccu guucggcggg auguccuggu uuucucaaau ccuuaucgga     1980

acacuccuga uguggcuggg ccucaacacg aagaauggga gcauuucccu gaugugucuu     2040

gcgcugggag gcgugcucau cuuccugagc accgccguuu cugcu                     2085


<210>  2280
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2280
aagaaaagac ggggcgccga caccagcgug gggaucgucg gacugcuccu gacaacggcu       60

auggccgcgg agguuacccg cagaggcucc gcuuacuaua uguaccuuga ucggaacgac      120

gccggggaag ccauuucuuu ccccacaacc cugggaauga auaagugcua uauccagauu      180

auggaucucg gccacaugug ugacgcuaca augagcuacg agugcccuau gcuggaugaa      240

gggguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

ggaacguguc aucacaaaaa gggcgaagcc cgcagaucuc ggcgcgcugu uacccuuccc      360

agccauucca caagaaaacu gcaaacccgg ucucagacau ggcucgagag ccgcgaguac      420

accaagcacc ugaucagagu ggaaaauugg auuuuucgga acccuggcuu cgcccuggcg      480

gccgcugcca ucgcuuggcu ccuggggucc agcacgucuc agaaggucau cuaucuugug      540

augauucugc ucaucgcccc cgcguacucc auucgcugca ucggaguuag caauagagau      600

uuuguggagg gcaugucugg gggaacaugg gucgacauug ugcuggaaca uggcgggugu      660

gugaccguca uggcccagga uaaaccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg cugagguccg gagcuacugc uaugaagccu cuauuuccga uauggcuagc      780

gacucccgcu gucccacgca aggagaggcc uaccuggaca agcagucuga uacccaguau      840

gugugcaaaa gaacacucgu ugaccggggc uggggcaaug gguguggacu guucggcaag      900

gggagccugg ugaccugcgc caaauuugcu ugcuccaaga agaugacagg aaaaagcauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggcuccca acacagcggg     1020

augauuguga augauaccgg acaugaaacg gacgagaacc gcgcgaaggu ugagaucaca     1080

cccaauucuc cccgggccga agcuacccuu ggcggcuucg ggagccuggg acucgauugu     1140

gagccuagaa ccggccugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc cggggccgau     1260

accggaacac cccauuggaa caauaaagag gcucuggucg aauuuaagga cgcccacgcg     1320

aaacgccaga cgguggucgu gcucggcucu caggaggggg cuguucauac cgcccuggcc     1380

ggcgcucuug aggccgaaau ggauggagcu aagggccggc ugagcuccgg gcaccucaag     1440

ugcagacuga aaauggacaa acuucgccug aagggaguga gcuauucucu cuguacagcc     1500

gcguucaccu uuacaaagau cccggccgag acccugcaug gcacggucac cguggaaguu     1560

caguacgcug ggacagacgg accuugcaaa gugcccgccc aaauggcugu cgauaugcag     1620

acccugacac cugugggccg gcucauuacc gccaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucgggg auucuuacau cguuauuggc     1740

gucggagaga agaaaaucac ccaccacugg cauagauccg gcagcacaau ugggaaggcg     1800

uuugaagcca ccgugcgcgg agcuaaacgg auggccgugc ugggcgacac cgcuugggau     1860

uucggguccg ucggaggcgc ccucaauucu cuggggaagg gcauccacca gauuuucgga     1920

gccgcuuuua aaagccuguu cggcgggaug uccugguuuu cucaaauccu uaucggaaca     1980

cuccugaugu ggcugggccu caacacgaag aaugggagca uuucccugau gugucuugcg     2040

cugggaggcg ugcucaucuu ccugagcacc gccguuucug cu                        2082


<210>  2281
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2281
aagagacggg gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug       60

gccgcggagg uuacccgcag aggcuccgcu uacuauaugu accuugaucg gaacgacgcc      120

ggggaagcca uuucuuuccc cacaacccug ggaaugaaua aaugcuauau ccagauuaug      180

gaucucggcc acauguguga cgcuacaaug agcuacgagu gcccuaugcu ggaugaaggg      240

guggagccgg acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga      300

acgugucauc acaagaaagg cgaagcccgc agaucucggc gcgcuguuac ccuucccagc      360

cauuccacaa gaaagcugca aacccggucu cagacauggc ucgagagccg cgaguacacc      420

aaacaccuga ucagagugga aaauuggauu uuucggaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggguccagc acgucucaga aggucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu cgcugcaucg gaguuagcaa uagagauuuu      600

guggagggca ugucuggggg aacauggguc gacauugugc uggaacaugg cgggugugug      660

accgucaugg cccaggauaa gccuacaguu gacaucgagc uugugaccac cacagugucc      720

aacauggcug agguccggag cuacugcuau gaagccucua uuuccgauau ggcuagcgac      780

ucccgcuguc ccacgcaagg agaggccuac cuggacaaac agucugauac ccaguaugug      840

ugcaagagaa cacucguuga ccggggcugg ggcaaugggu guggacuguu cggcaaaggg      900

agccugguga ccugcgccaa guuugcuugc uccaaaaaga ugacaggaaa gagcauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gcucccaaca cagcgggaug     1020

auugugaaug auaccggaca ugaaacggac gagaaccgcg cgaaaguuga gaucacaccc     1080

aauucucccc gggccgaagc uacccuuggc ggcuucggga gccugggacu cgauugugag     1140

ccuagaaccg gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaagcacugg     1200

cucgugcaua aagaaugguu ccacgacauu ccccugccuu ggcacgccgg ggccgauacc     1260

ggaacacccc auuggaacaa uaaggaggcu cuggucgaau uuaaagacgc ccacgcgaag     1320

cgccagacgg uggucgugcu cggcucucag gagggggcug uucauaccgc ccuggccggc     1380

gcucuugagg ccgaaaugga uggagcuaaa ggccggcuga gcuccgggca ccucaagugc     1440

agacugaaga uggacaaacu ucgccugaag ggagugagcu auucucucug uacagccgcg     1500

uucaccuuua caaaaauccc ggccgagacc cugcauggca cggucaccgu ggaaguucag     1560

uacgcuggga cagacggacc uugcaaggug cccgcccaaa uggcugucga uaugcagacc     1620

cugacaccug ugggccggcu cauuaccgcc aaccccguga ucacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugacccgccu uucggggauu cuuacaucgu uauuggcguc     1740

ggagagaaga agaucaccca ccacuggcau agauccggca gcacaauugg gaaagcguuu     1800

gaagccaccg ugcgcggagc uaagcggaug gccgugcugg gcgacaccgc uugggauuuc     1860

ggguccgucg gaggcgcccu caauucucug gggaaaggca uccaccagau uuucggagcc     1920

gcuuuuaaga gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc     1980

cugauguggc ugggccucaa cacgaaaaau gggagcauuu cccugaugug ucuugcgcug     2040

ggaggcgugc ucaucuuccu gagcaccgcc guuucugcu                            2079


<210>  2282
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2282
agacggggcg ccgacaccag cguggggauc gucggacugc uccugacaac ggcuauggcc       60

gcggagguua cccgcagagg cuccgcuuac uauauguacc uugaucggaa cgacgccggg      120

gaagccauuu cuuuccccac aacccuggga augaauaagu gcuauaucca gauuauggau      180

cucggccaca ugugugacgc uacaaugagc uacgagugcc cuaugcugga ugaaggggug      240

gagccggacg auguggacug uuggugcaac accacaucca ccugggucgu guauggaacg      300

ugucaucaca aaaagggcga agcccgcaga ucucggcgcg cuguuacccu ucccagccau      360

uccacaagaa aacugcaaac ccggucucag acauggcucg agagccgcga guacaccaag      420

caccugauca gaguggaaaa uuggauuuuu cggaacccug gcuucgcccu ggcggccgcu      480

gccaucgcuu ggcuccuggg guccagcacg ucucagaaag ucaucuaucu ugugaugauu      540

cugcucaucg cccccgcgua cuccauucgc ugcaucggag uuagcaauag agauuuugug      600

gagggcaugu cugggggaac augggucgac auugugcugg aacauggcgg gugugugacc      660

gucauggccc aggauaagcc uacaguugac aucgagcuug ugaccaccac aguguccaac      720

auggcugagg uccggagcua cugcuaugaa gccucuauuu ccgauauggc uagcgacucc      780

cgcuguccca cgcaaggaga ggccuaccug gacaagcagu cugauaccca guaugugugc      840

aaaagaacac ucguugaccg gggcuggggc aaugggugug gacuguucgg caaggggagc      900

cuggugaccu gcgccaaauu ugcuugcucc aagaaaauga caggaaagag cauccagccu      960

gaaaaccucg aguacagaau caugcugucu guccacggcu cccaacacag cgggaugauu     1020

gugaaugaua ccggacauga aacggacgag aaccgcgcga agguugagau cacacccaau     1080

ucuccccggg ccgaagcuac ccuuggcggc uucgggagcc ugggacucga uugugagccu     1140

agaaccggcc uggacuuuuc cgaucuuuau uaccugacaa ugaacaauaa acacuggcuc     1200

gugcauaagg aaugguucca cgacauuccc cugccuuggc acgccggggc cgauaccgga     1260

acaccccauu ggaacaauaa agaggcucug gucgaauuua aggacgccca cgcgaaacgc     1320

cagacggugg ucgugcucgg cucucaggag ggggcuguuc auaccgcccu ggccggcgcu     1380

cuugaggccg aaauggaugg agcuaagggc cggcugagcu ccgggcaccu caaaugcaga     1440

cugaagaugg acaaacuucg ccugaaggga gugagcuauu cucucuguac agccgcguuc     1500

accuuuacaa agaucccggc cgagacccug cauggcacgg ucaccgugga aguucaguac     1560

gcugggacag acggaccuug caaagugccc gcccaaaugg cugucgauau gcagacccug     1620

acaccugugg gccggcucau uaccgccaac cccgugauca cggaguccac agaaaacagc     1680

aagaugaugc uggagcuuga cccgccuuuc ggggauucuu acaucguuau uggcgucgga     1740

gagaaaaaga ucacccacca cuggcauaga uccggcagca caauugggaa ggcguuugaa     1800

gccaccgugc gcggagcuaa acggauggcc gugcugggcg acaccgcuug ggauuucggg     1860

uccgucggag gcgcccucaa uucucugggg aagggcaucc accagauuuu cggagccgcu     1920

uuuaaaagcc uguucggcgg gauguccugg uuuucucaaa uccuuaucgg aacacuccug     1980

auguggcugg gccucaacac gaagaauggg agcauuuccc ugaugugucu ugcgcuggga     2040

ggcgugcuca ucuuccugag caccgccguu ucugcu                               2076


<210>  2283
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2283
agaggcgccg acaccagcgu ggggaucguc ggacugcucc ugacaacggc uauggccgcg       60

gagguuaccc ggcgcggcuc cgcuuacuau auguaccuug auagaaacga cgccggggaa      120

gccauuucuu uccccacaac ccugggaaug aauaagugcu auauccagau uauggaucuc      180

ggccacaugu gugacgcuac aaugagcuac gagugcccua ugcuggauga agggguggag      240

ccggacgaug uggacuguug gugcaacacc acauccaccu gggucgugua uggaacgugu      300

caucacaaaa agggcgaagc ccggcgcucu agacgggcug uuacccuucc cagccauucc      360

acacgcaaac ugcaaaccag aucucagaca uggcucgaga gccgggagua caccaagcac      420

cugauccgcg uggaaaauug gauuuuuaga aacccuggcu ucgcccuggc ggccgcugcc      480

aucgcuuggc uccugggguc cagcacgucu cagaaaguca ucuaucuugu gaugauucug      540

cucaucgccc ccgcguacuc cauucggugc aucggaguua gcaaucgcga uuuuguggag      600

ggcaugucug ggggaacaug ggucgacauu gugcuggaac auggcgggug ugugaccguc      660

auggcccagg auaagccuac aguugacauc gagcuuguga ccaccacagu guccaacaug      720

gcugagguca gaagcuacug cuaugaagcc ucuauuuccg auauggcuag cgacucccgg      780

ugucccacgc aaggagaggc cuaccuggac aagcagucug auacccagua ugugugcaaa      840

cgcacacucg uugacagagg cuggggcaau ggguguggac uguucggcaa ggggagccug      900

gugaccugcg ccaaauuugc uugcuccaag aaaaugacag gaaagagcau ccagccugaa      960

aaccucgagu accggaucau gcugucuguc cacggcuccc aacacagcgg gaugauugug     1020

aaugauaccg gacaugaaac ggacgagaac agagcgaagg uugagaucac acccaauucu     1080

ccccgcgccg aagcuacccu uggcggcuuc gggagccugg gacucgauug ugagccucgg     1140

accggccugg acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug     1200

cauaaggaau gguuccacga cauuccccug ccuuggcacg ccggggccga uaccggaaca     1260

ccccauugga acaauaaaga ggcucugguc gaauuuaagg acgcccacgc gaaaagacag     1320

acgguggucg ugcucggcuc ucaggagggg gcuguucaua ccgcccuggc cggcgcucuu     1380

gaggccgaaa uggauggagc uaagggccgc cugagcuccg ggcaccucaa augccggcug     1440

aagauggaca aacuuagacu gaagggagug agcuauucuc ucuguacagc cgcguucacc     1500

uuuacaaaga ucccggccga gacccugcau ggcacgguca ccguggaagu ucaguacgcu     1560

gggacagacg gaccuugcaa agugcccgcc caaauggcug ucgauaugca gacccugaca     1620

ccugugggcc gccucauuac cgccaacccc gugaucacgg aguccacaga aaacagcaag     1680

augaugcugg agcuugaccc gccuuucggg gauucuuaca ucguuauugg cgucggagag     1740

aaaaagauca cccaccacug gcaucggucc ggcagcacaa uugggaaggc guuugaagcc     1800

accgugagag gagcuaaacg cauggccgug cugggcgaca ccgcuuggga uuucgggucc     1860

gucggaggcg cccucaauuc ucuggggaag ggcauccacc agauuuucgg agccgcuuuu     1920

aaaagccugu ucggcgggau guccugguuu ucucaaaucc uuaucggaac acuccugaug     1980

uggcugggcc ucaacacgaa gaaugggagc auuucccuga ugugucuugc gcugggaggc     2040

gugcucaucu uccugagcac cgccguuucu gcu                                  2073


<210>  2284
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2284
ggcgccgaca ccagcguggg gaucgucgga cugcuccuga caacggcuau ggccgcggag       60

guuaccagac ggggcuccgc uuacuauaug uaccuugauc gcaacgacgc cggggaagcc      120

auuucuuucc ccacaacccu gggaaugaau aagugcuaua uccagauuau ggaucucggc      180

cacaugugug acgcuacaau gagcuacgag ugcccuaugc uggaugaagg gguggagccg      240

gacgaugugg acuguuggug caacaccaca uccaccuggg ucguguaugg aacgugucau      300

cacaaaaagg gcgaagccag acggucucgc agagcuguua cccuucccag ccauuccaca      360

cggaaacugc aaacccgcuc ucagacaugg cucgagagca gagaguacac caagcaccug      420

auccgggugg aaaauuggau uuuucgcaac ccuggcuucg cccuggcggc cgcugccauc      480

gcuuggcucc ugggguccag cacgucucag aaagucaucu aucuugugau gauucugcuc      540

aucgcccccg cguacuccau uagaugcauc ggaguuagca aucgggauuu uguggagggc      600

augucugggg gaacaugggu cgacauugug cuggaacaug gcgggugugu gaccgucaug      660

gcccaggaua agccuacagu ugacaucgag cuugugacca ccacaguguc caacauggcu      720

gagguccgca gcuacugcua ugaagccucu auuuccgaua uggcuagcga cuccagaugu      780

cccacgcaag gagaggccua ccuggacaag cagucugaua cccaguaugu gugcaaacgg      840

acacucguug accgcggcug gggcaauggg uguggacugu ucggcaaggg gagccuggug      900

accugcgcca aauuugcuug cuccaagaaa augacaggaa agagcaucca gccugaaaac      960

cucgaguaca gaaucaugcu gucuguccac ggcucccaac acagcgggau gauugugaau     1020

gauaccggac augaaacgga cgagaaccgg gcgaagguug agaucacacc caauucuccc     1080

agagccgaag cuacccuugg cggcuucggg agccugggac ucgauuguga gccucgcacc     1140

ggccuggacu uuuccgaucu uuauuaccug acaaugaaca auaaacacug gcucgugcau     1200

aaggaauggu uccacgacau uccccugccu uggcacgccg gggccgauac cggaacaccc     1260

cauuggaaca auaaagaggc ucuggucgaa uuuaaggacg cccacgcgaa acggcagacg     1320

guggucgugc ucggcucuca ggagggggcu guucauaccg cccuggccgg cgcucuugag     1380

gccgaaaugg auggagcuaa gggcagacug agcuccgggc accucaaaug ccgccugaag     1440

auggacaaac uucggcugaa gggagugagc uauucucucu guacagccgc guucaccuuu     1500

acaaagaucc cggccgagac ccugcauggc acggucaccg uggaaguuca guacgcuggg     1560

acagacggac cuugcaaagu gcccgcccaa auggcugucg auaugcagac ccugacaccu     1620

gugggcagac ucauuaccgc caaccccgug aucacggagu ccacagaaaa cagcaagaug     1680

augcuggagc uugacccgcc uuucggggau ucuuacaucg uuauuggcgu cggagagaaa     1740

aagaucaccc accacuggca ucgcuccggc agcacaauug ggaaggcguu ugaagccacc     1800

gugcggggag cuaaaagaau ggccgugcug ggcgacaccg cuugggauuu cggguccguc     1860

ggaggcgccc ucaauucucu ggggaagggc auccaccaga uuuucggagc cgcuuuuaaa     1920

agccuguucg gcgggauguc cugguuuucu caaauccuua ucggaacacu ccugaugugg     1980

cugggccuca acacgaagaa ugggagcauu ucccugaugu gucuugcgcu gggaggcgug     2040

cucaucuucc ugagcaccgc cguuucugcu                                      2070


<210>  2285
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2285
gccgacacca gcgugggcau cgucgggcug cuccugacaa cggcuauggc cgcggagguu       60

accagacggg gauccgcuua cuauauguac cuugaucgca acgacgccgg cgaagccauu      120

ucuuucccca caacccuggg gaugaauaag ugcuauaucc agauuaugga ucucggacac      180

augugugacg cuacaaugag cuacgagugc ccuaugcugg augaaggcgu ggagccggac      240

gauguggacu guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac      300

aaaaagggag aagccagacg gucucgcaga gcuguuaccc uucccagcca uuccacacgg      360

aaacugcaaa cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc      420

cggguggaaa auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu      480

uggcuccugg gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc      540

gcccccgcgu acuccauuag augcaucggg guuagcaauc gggauuuugu ggagggaaug      600

ucuggcggga caugggucga cauugugcug gaacauggag gcugugugac cgucauggcc      660

caggauaagc cuacaguuga caucgagcuu gugaccacca caguguccaa cauggcugag      720

guccgcagcu acugcuauga agccucuauu uccgauaugg cuagcgacuc cagauguccc      780

acgcaagggg aggccuaccu ggacaagcag ucugauaccc aguaugugug caaacggaca      840

cucguugacc gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc      900

ugcgccaaau uugcuugcuc caagaaaaug acaggaaaga gcauccagcc ugaaaaccuc      960

gaguacagaa ucaugcuguc uguccacggg ucccaacaca gcggcaugau ugugaaugau     1020

accgggcaug aaacggacga gaaccgggcg aagguugaga ucacacccaa uucucccaga     1080

gccgaagcua cccuuggagg cuucggcagc cuggggcucg auugugagcc ucgcaccgga     1140

cuggacuuuu ccgaucuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag     1200

gaaugguucc acgacauucc ccugccuugg cacgccggcg ccgauaccgg gacaccccau     1260

uggaacaaua aagaggcucu ggucgaauuu aaggacgccc acgcgaaacg gcagacggug     1320

gucgugcucg gaucucagga gggcgcuguu cauaccgccc uggccggggc ucuugaggcc     1380

gaaauggaug gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug     1440

gacaagcugc ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca     1500

aagaucccgg ccgagacccu gcauggaacg gucaccgugg aaguucagua cgcuggcaca     1560

gacgggccuu gcaaagugcc cgcccaaaug gcugucgaua ugcagacccu gacaccugug     1620

ggaagacuca uuaccgccaa ccccgugauc acggagucca cagaaaacag caagaugaug     1680

cuggagcuug acccgccuuu cggcgauucu uacaucguua uuggggucgg cgagaaaaag     1740

aucacccacc acuggcaucg cuccggaagc acaauuggca aggcguuuga agccaccgug     1800

agaggggcua aacggauggc cgugcuggga gacaccgcuu gggauuucgg cuccgucggg     1860

ggagcccuca auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc     1920

cuguucggag gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug     1980

ggacucaaca cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc     2040

aucuuccuga gcaccgccgu uucugcu                                         2067


<210>  2286
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2286
gacaccagcg ugggcaucgu cgggcugcuc cugacaacgg ccauggcugc cgagguuacc       60

agacggggau ccgcguacua uauguaccuu gaucgcaacg acgcuggcga agccauuucu      120

uuccccacaa cccuggggau gaauaagugc uauauccaga uuauggaucu cggacacaug      180

ugugacgcca caaugagcua cgagugcccu augcuggaug aaggcgugga gccggacgau      240

guggacuguu ggugcaacac cacauccacc ugggucgugu augggacgug ucaucacaaa      300

aagggagaag cuagacgguc ucgcagagcc guuacccuuc ccagccauuc cacacggaaa      360

cugcaaaccc gcucucagac auggcucgag agcagagagu acaccaagca ccugauccgg      420

guggaaaauu ggauuuuucg caacccuggc uucgcucugg ccgcggccgc uaucgccugg      480

cuccugggcu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcu      540

cccgccuacu ccauuagaug caucgggguu agcaaucggg auuuugugga gggaaugucu      600

ggcgggacau gggucgacau ugugcuggaa cauggaggcu gugugaccgu cauggcgcag      660

gauaagccua caguugacau cgagcuugug accaccacag uguccaacau ggccgagguc      720

cgcagcuacu gcuaugaagc uagcauuucc gauauggcca gcgacuccag augucccacg      780

caaggggagg cuuaccugga caagcagucu gauacccagu augugugcaa acggacacuc      840

guugaccgcg gauggggcaa uggcuguggg cuguucggaa agggcagccu ggugaccugc      900

gccaaauuug ccugcuccaa gaaaaugaca gggaagucua uccagccuga aaaccucgag      960

uacagaauca ugcugucugu ccacggaucc caacacagcg gcaugauugu gaaugauacc     1020

gggcaugaaa cggacgagaa ccgggcuaag guugagauca cacccaauuc ucccagagcg     1080

gaagccaccc uuggaggcuu cggcagccug gggcucgauu gugagccucg caccggacug     1140

gacuuuuccg aucuuuauua ccugacaaug aacaauaaac acuggcucgu gcauaaggaa     1200

ugguuccacg acauuccccu gccuuggcac gcuggcgccg auaccgggac accccauugg     1260

aacaauaaag aggcccuggu cgaauuuaag gacgcucacg ccaaacggca gacggugguc     1320

gugcucggau cucaggaggg cgcgguucau accgcucugg ccggggcccu ugaggcugaa     1380

auggauggcg ccaagggaag acugagcucc ggccaccuca aaugccgccu gaagauggac     1440

aagcugcggc ugaaaggggu gagcuauucu cucuguacag cugccuucac cuuuacaaag     1500

aucccggcgg agacccugca uggaacgguc accguggaag uucaguacgc cggcacagac     1560

gggccuugca aagugcccgc ucaaauggcc gucgauaugc agacccugac accuguggga     1620

agacucauua ccgcuaaccc cgugaucacg gaguccacag aaaacagcaa gaugaugcug     1680

gagcuugacc cgccuuucgg cgauucuuac aucguuauug gggucggcga gaaaaagauc     1740

acccaccacu ggcaucgcuc cggaagcaca auuggcaagg ccuuugaagc gaccgugaga     1800

ggggccaaac ggauggcugu gcugggagac accgccuggg auuucggcuc cgucggggga     1860

gcucucaauu cucugggcaa ggggauccac cagauuuucg gcgccgccuu uaaaagccug     1920

uucggaggca uguccugguu uucucaaauc cuuaucggga cacuccugau guggcuggga     1980

cucaacacga agaauggcag cauuucccug augugucuug cucuuggggg agugcucauc     2040

uuccugagca ccgcgguuuc ugcc                                            2064


<210>  2287
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  2287
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc c                                               2061


<210>  2288
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2288
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccgc gguuaccaga       60

cggggauccg cuuacuauau guaccuugac cgcaacgaug ccggcgaggc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugcuacaa ugagcuacga augcccuaug cuggacgagg gcguggaacc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaggcca gacggucucg cagagcuguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgaaagc agagaguaca ccaagcaccu gauccgggug      420

gagaauugga uuuuucgcaa cccuggcuuc gcccuggcgg ccgcugccau cgcuuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgccccc      540

gcguacucca uuagaugcau cgggguuagc aaucgggacu uuguggaagg aaugucuggc      600

gggacauggg ucgauauugu gcuggagcau ggaggcugug ugaccgucau ggcccaggac      660

aagccuacag uugauaucga acuugugacc accacagugu ccaacauggc ugagguccgc      720

agcuacugcu augaggccuc uauuuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaagccu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugcuu gcuccaagaa aaugacaggg aagucuaucc agccugagaa ccucgaauac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugagacgg augaaaaccg ggcgaagguu gagaucacac cgaauagccc cagagccgag     1080

gcuacccuug gaggcuucgg cagccugggg cucgacugug aaccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggagugg     1200

uuccacgaua uuccccugcc uuggcacgcc ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagaag cucuggucga guuuaaggau gcccacgcga aacggcagac gguggucgug     1320

cucggaucuc aggaaggcgc uguucauacc gcccuggccg gggcucuuga ggccgagaug     1380

gacggcgcua agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagccg cguucaccuu uacaaagauc     1500

ccggccgaaa cccugcaugg aacggucacc guggagguuc aguacgcugg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcuguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg ccaaccccgu gaucacggaa uccacagaga acagcaagau gaugcuggaa     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggcgu uugaggccac cgugagaggg     1800

gcuaaacgga uggccgugcu gggagauacc gcuugggacu ucggcuccgu cgggggagcc     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcgc uugggggagu gcucaucuuc     2040

cugagcaccg ccguuucugc u                                               2061


<210>  2289
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2289
agaggcgccg acaccagcgu ggggaucguc ggacugcucc ugacaacggc uauggccgcg       60

gcuguuaccc ggcgcggcuc cgccuacuau auguaccuug auagaaacga cgccggggag      120

gcuauuucuu uccccacaac ccugggaaug aauaagugcu auauccagau uauggaucuc      180

ggccacaugu gugacgccac aaugagcuac gaaugcccua ugcuggauga ggggguggaa      240

ccggacgaug uggacuguug gugcaacacc acauccaccu gggucgugua uggaacgugu      300

caucacaaaa agggcgaggc ucggcgcucu agacgggccg uuacccuucc cagccauucc      360

acacgcaaac ugcaaaccag aucucagaca uggcucgaaa gccgggagua caccaagcac      420

cugauccgcg uggagaauug gauuuuuaga aacccuggcu ucgcgcuggc cgcugccgcu      480

aucgccuggc uccugggguc cagcacgucu cagaaaguca ucuaucuugu gaugauucug      540

cucaucgcgc ccgccuacuc cauucggugc aucggaguua gcaaucgcga uuuuguggaa      600

ggcaugucug ggggaacaug ggucgacauu gugcuggagc auggcgggug ugugaccguc      660

auggcucagg auaagccuac aguugacauc gaacuuguga ccaccacagu guccaacaug      720

gccgagguca gaagcuacug cuaugaggcu ucuauuuccg auauggccag cgacucccgg      780

ugucccacgc aaggagaagc cuaccuggac aagcagucug auacccagua ugugugcaaa      840

cgcacacucg uugacagagg cuggggcaau ggguguggac uguucggcaa ggggagccug      900

gugaccugcg cuaaauuugc gugcuccaag aaaaugacag gaaagagcau ccagccugag      960

aaccucgaau accggaucau gcugucuguc cacggcuccc aacacagcgg gaugauugug     1020

aaugauaccg gacaugagac ggacgaaaac agagccaagg uugagaucac acccaauucu     1080

ccccgcgcug aggccacccu uggcggcuuc gggagccugg gacucgauug ugaaccucgg     1140

accggccugg acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug     1200

cauaaggagu gguuccacga cauuccccug ccuuggcacg ccggggcuga uaccggaaca     1260

ccccauugga acaauaaaga agcccugguc gaguuuaagg acgcgcacgc uaaaagacag     1320

acgguggucg ugcucggcuc ucaggaaggg gccguucaua ccgcccuggc uggcgcccuu     1380

gaggcugaga uggauggagc caagggccgc cugagcuccg ggcaccucaa augccggcug     1440

aagauggaca aacuuagacu gaagggagug agcuauucuc ucuguacagc ggccuucacc     1500

uuuacaaaga ucccggcuga aacccugcau ggcacgguca ccguggaggu ucaguacgcc     1560

gggacagacg gaccuugcaa agugcccgcu caaauggccg ucgauaugca gacccugaca     1620

ccugugggcc gccucauuac cgcgaacccc gugaucacgg aauccacaga gaacagcaag     1680

augaugcugg aacuugaccc gccuuucggg gauucuuaca ucguuauugg cgucggagag     1740

aaaaagauca cccaccacug gcaucggucc ggcagcacaa uugggaaggc cuuugaggcu     1800

accgugagag gagccaaacg cauggcugug cugggcgaca ccgccuggga uuucgggucc     1860

gucggaggcg cccucaauuc ucuggggaag ggcauccacc agauuuucgg agcugcguuu     1920

aaaagccugu ucggcgggau guccugguuu ucucaaaucc uuaucggaac acuccugaug     1980

uggcugggcc ucaacacgaa gaaugggagc auuucccuga ugugucuugc ccugggaggc     2040

gugcucaucu uccugagcac cgcuguuucu gcc                                  2073


<210>  2290
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2290
agaggccuga gcucccggaa gcgcagaucu cacgacgugc ucaccgucca guuccugauc       60

cuugggaugc ugcucaugac aggaggcguu acgcggcgcg ggagcgccua cuauauguac      120

cuggauagaa acgacgcugg agaggccauu uccuuuccca ccacacuugg caugaauaaa      180

ugcuauaucc aaauuaugga ucuggggcau augugugacg cgaccauguc uuacgaaugc      240

ccuaugcucg augagggagu ggaaccggac gauguggacu guuggugcaa cacaaccagc      300

acaugggucg uguauggcac cugucaccau aagaaaggcg aggcucggcg cuccagacgg      360

gccguuacgc ugcccucuca cagcacccgc aagcugcaga caagauccca gaccuggcuc      420

gaaagccggg aguacacaaa acaucugauc cgcguggaga auuggauuuu cagaaacccu      480

ggguuugccc uugcugccgc ugccaucgcg uggcugcucg gaucuuccac cagccagaag      540

gucaucuauc uggugaugau ucuucugauc gcccccgcuu acucuauucg gugcaucggc      600

guuuccaauc gcgauuucgu ggaagggaug agcggaggca cgugggucga cauugugcuc      660

gagcacgggg gaugugugac agucauggcc caagauaagc cuaccguuga caucgaacug      720

gugacaacca ccgugucuaa cauggcugag gucagauccu acugcuauga ggccagcauu      780

uccgauaugg cgucugacag ccgguguccc acacagggcg aagccuaccu ggacaaacag      840

uccgauacgc aguaugugug caagagaacc cucguugacc gcggcugggg gaauggaugu      900

ggccuguuug ggaaaggaag ccuugugaca ugcgcuaagu ucgccugcuc uaaaaagaug      960

accggcaagu ccauccaacc ugagaaccug gaauaccgga ucaugcucag cguccacggg     1020

ucucagcaua gcggaaugau ugugaaugau acaggccacg agaccgacga aaacagagcu     1080

aaaguugaga ucacgcccaa uuccccgcgc gccgaggcca cacugggcgg guuuggaucu     1140

cuuggccugg auugugaacc ucggaccggg cucgacuuca gcgaucugua uuaccugacc     1200

augaacaaua agcauuggcu cgugcacaaa gagugguuuc acgacauucc ccugccuugg     1260

caugcuggag cggauacagg cacaccccac uggaacaaua aggaagcccu ugucgaguuc     1320

aaagacgcuc augccaagag acagacagug gucgugcugg ggucccagga aggcgccguu     1380

cacacggcuc ucgccggagc gcuggaggcu gagauggaug gcgccaaagg gcgccuuagc     1440

ucuggacauc ugaagugccg gcucaagaug gacaaacuga gacugaaggg cguguccuau     1500

agccucugua ccgccgcuuu uacauucacc aaaaucccgg ccgaaacacu gcacgggacc     1560

gucacggugg agguucaaua cgcuggaacc gacggcccuu gcaaggugcc cgcccagaug     1620

gcggucgaua ugcagacacu uaccccugug gggcgccuga uuacagccaa ccccgugauc     1680

accgaaucua cggagaacuc caaaaugaug cucgaacugg aucccccuuu uggcgacagc     1740

uacaucguua uuggagucgg cgagaagaag aucacacacc auuggcaccg guccgggucu     1800

accauuggaa aggcuuucga ggccacagug agaggcgcua aacgcauggc cgugcugggg     1860

gacaccgcgu gggauuucgg aagcgucggc ggggcccuua auucccucgg caaaggaauc     1920

caucaaauuu uuggcgcugc cuucaagucu cuguuugggg gaaugagcug guucucccag     1980

auccugaucg gcacccuccu gauguggcuu gggcugaaca ccaaaaaugg aagcauuucu     2040

cucauguguc uggcucuggg cggggugcuu aucuuucucu ccacggccgu uagcgcc        2097


<210>  2291
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2291
agaggccuga gcucccggaa gcgcagaucu cacgacgugc ucaccgucca guuccugauc       60

cuugggaugc ugcucaugac aggagccguu acgcggcgcg gcagcgcuua cuauauguac      120

cuggauagaa acgacgccgg ggaggcgauu uccuuuccca ccacacuugg aaugaauaaa      180

ugcuauaucc aaauuaugga ucugggccau augugugacg cuaccauguc uuacgaaugc      240

ccuaugcucg augagggggu ggaaccggac gauguggacu guuggugcaa cacaaccagc      300

acaugggucg uguauggaac cugucaccau aagaaaggcg aggcccggcg cuccagacgg      360

gccguuacgc ugcccucuca cagcacccgc aagcugcaga caagauccca gaccuggcuc      420

gaaagccggg aguacacaaa acaucugauc cgcguggaga auuggauuuu cagaaacccu      480

ggcuuugcuc uugccgcugc cgcgaucgcc uggcugcucg ggucuuccac cagccagaag      540

gucaucuauc uggugaugau ucuucugauc gcucccgccu acucuauucg gugcaucgga      600

guuuccaauc gcgauuucgu ggaaggcaug agcgggggaa cgugggucga cauugugcuc      660

gagcacggcg ggugugugac agucauggcu caagauaagc cuaccguuga caucgaacug      720

gugacaacca ccgugucuaa cauggccgag gucagauccu acugcuauga ggcgagcauu      780

uccgauaugg ccucugacag ccgguguccc acacagggag aggcuuaccu ggacaaacag      840

uccgauacgc aguaugugug caagagaacc cucguugacc gcggcugggg caaugggugu      900

ggacuguuug gcaaagggag ccuugugaca ugcgccaagu ucgcuugcuc uaaaaagaug      960

accggaaagu ccauccaacc ugaaaaccug gaauaccgga ucaugcucag cguccacggc     1020

ucucagcaua gcgggaugau ugugaaugau acaggacacg agaccgacga aaacagagcc     1080

aaaguugaga ucacgcccaa uuccccgcgc gccgaggcua cacugggcgg cuuugggucu     1140

cuuggacugg auugugaacc ucggaccggc cucgacuuca gcgaucugua uuaccugacc     1200

augaacaaua agcauuggcu cgugcacaaa gagugguuuc acgacauucc ccugccuugg     1260

caugcggggg ccgauacagg aacaccccac uggaacaaua aggaagcucu ugucgaguuc     1320

aaagacgccc augccaagag acagacagug gucgugcugg gcucccagga aggggcuguu     1380

cacacggccc ucgcgggcgc ucuggaggcc gagauggaug gagccaaagg ccgccuuagc     1440

ucugggcauc ugaagugccg gcucaagaug gacaaacuga gacugaaggg aguguccuau     1500

agccucugua ccgcugccuu uacauucacc aaaaucccgg cugaaacacu gcacggcacc     1560

gucacggugg agguucaaua cgccgggacc gacggaccuu gcaaggugcc cgcgcagaug     1620

gccgucgaua ugcagacacu uaccccugug ggccgccuga uuacagcuaa ccccgugauc     1680

accgaaucua cggagaacuc caaaaugaug cucgaacugg aucccccuuu uggggacagc     1740

uacaucguua uuggcgucgg agagaagaag aucacacacc auuggcaccg guccggcucu     1800

accauuggga aagccuucga ggcuacagug agaggagcca agcgcauggc ggugcugggc     1860

gacaccgccu gggauuucgg gagcgucgga ggcgcucuua auucccucgg gaaaggcauc     1920

caucaaauuu uuggagccgc uuucaagucu cuguuuggcg ggaugagcug guucucccag     1980

auccugaucg gaacccuccu gauguggcuu ggccugaaca ccaaaaaugg gagcauuucu     2040

cucauguguc uggcccuggg aggcgugcuu aucuuucucu ccacggccgu uagcgcu        2097


<210>  2292
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long CDS opt

<400>  2292
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaau auggccgaag ucagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguugaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcgca cgcuaagaga cagacggugg ucgugcucgg cucucaggag     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccgugauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcgugcuca ucuuccugag caccgcuguu     2100

ucugccgacg uggggugcuc cgucgauuuu agcaaa                               2136


<210>  2293
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long CDS opt

<400>  2293
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca caugugacgc caccaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

acaaccucca cgugggucgu guauggaacc ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacacu ucccagccau uccacccgca agcugcaaac aagaucucag      420

accuggcucg agagccggga auacacgaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcaca      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac cugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggcuc aggauaagcc uaccguggac      720

auugagcuug ugaccacaac ggucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cccaaggaga agccuaccug      840

gacaaacagu cugauacaca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggucacau gcgcuaaguu ugcgugcucc      960

aagaaaauga ccggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

gugcacggcu cccaacacag cgggaugauc guuaacgaua cgggacauga gacagacgag     1080

aauagagcca aaguggaaau uaccccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggacaggcc uggacuuuuc cgaucuuuau     1200

uaccugacca ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauacagga acgccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagaccgucg ugguucucgg cucucaggag     1380

ggggccgugc auacagcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac cgcggccuuc acauuuacca aaauuccggc ugaaacgcug     1560

cauggcaccg ugacaguuga ggugcaguac gccgggaccg acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacacug accccugugg gccgccucau cacggcgaac     1680

cccguuauca cagaauccac cgagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acacagccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacgcuccug auguggcugg gccucaaugc caagaacggg     2040

agcaucuccc ugaugugucu ugcucuggga ggcguucuca uuuuccugag caccgccgug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2294
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long CDS opt

<400>  2294
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aacccuggga      180

gugaauaaau gccacgucca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cggugacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauua aaguugaaaa uuggaucuuu      480

agaaaccccg gcuucgcccu gguggcuguc gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaagg ugaucuaccu uguuaugauu cugcucaucg ccccugcgua uagcauucgg      600

ugcaucggag ugucuaaucg cgauuuuguc gagggcaugu ccgggggaac auggguggac      660

gugguccugg aacacggcgg guguguuacc gugauggccc aggauaaacc cacaguggac      720

aucgagcuug ucaccaccac agugagcaac auggcugagg uuagaucuua cugcuacgaa      780

gccuccauua gcgauauggc uuccgacucu cgguguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgugugc aaaagaacac ucgucgaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cuggugaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauccagccc gaaaaccucg aguaucggau uaugcugucc     1020

guucacggca gccaacauuc ugggaugauc ggauacgaaa cggaugagga cagagcuaag     1080

guggagguca caccuaauag cccgcgcgcg gaagccaccc uuggcggcuu cgggucccug     1140

ggacucgauu gugagccccg gaccggccug gacuuuucug aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccucu gcccuggcac     1260

gcuggggccg auaccggaac accucauugg aacaauaaag aggcccuggu cgaauuuaag     1320

gacgcucacg ccaagagaca gacggugguu gugcucggca gccaggaggg ggcgguccau     1380

accgcucugg ccggcgcccu ugaggcugaa auggauggag ccaaaggccg ccuguucucc     1440

gggcaccuca agugccggcu gaaaauggac aaacuuagac ugaagggagu gagcuauucu     1500

cucuguacag cugccuuuac cuucacaaag guucccgcgg agacccugca uggcacggug     1560

accgucgaag ugcaguacgc cgggacagac ggaccgugca agaucccugu gcaaauggcu     1620

guugauaugc agacccugac acccgucggc cgccucauca ccgccaaccc ugugauuacg     1680

gaguccacag aaaauagcaa aaugaugcug gagcuugacc cucccuuugg ggauucuuac     1740

aucgugauug gcgucggaga caagaaaauc acccaccacu ggcaucgguc cggcagcaca     1800

auugggaagg cuuucgaggc caccgugaga ggagcgaaac gcauggccgu ucugggcgau     1860

accgcuuggg acuucggguc cgugggaggc gucuuuaacu cucucgggaa gggcauccac     1920

cagaucuucg gagccgcuuu uaagagccug uucggcggga uguccugguu uucucaaauu     1980

cugaucggaa cacuucucgu guggcugggc cugaacacga aaaaugggag cauuucccuc     2040

accugucugg cccuuggagg cguuaugauc uuccugagca cagccguguc ugcugauguc     2100

gggugcuccg uggacuuuag caag                                            2124


<210>  2295
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long CDS opt

<400>  2295
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2296
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long CDS opt

<400>  2296
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aacccuggga      180

gugaauaaau gccacgucca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cggugacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauua aaguugaaaa uuggaucuuu      480

agaaaccccg gcuucgcccu gguggcuguc gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaagg ugaucuaccu uguuaugauu cugcucaucg ccccugcgua uagcauucgg      600

ugcaucggag ugucuaaucg cgauuuuguc gagggcaugu ccgggggaac auggguggac      660

gugguccugg aacacggcgg guguguuacc gugauggccc aggauaaacc cacaguggac      720

aucgagcuug ucaccaccac agugagcaac auggcugagg uuagaucuua cugcuacgaa      780

gccuccauua gcgauauggc uuccgacucu cgguguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgugugc aaaagaacac ucgucgaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cuggugaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauccagccc gaaaaccucg aguaucggau uaugcugucc     1020

guucacggca gccaacauuc ugggaugauc gugaaugaua cgggauacga aacagacgag     1080

aacagagcua aggucgaggu gaccccuaau agcccgcgcg cggaagccac ccuuggcggc     1140

uucggguccc ugggacucga uugugagccc cggacaggcc uggacuuuuc ugaucuuuau     1200

uaccugacca ugaacaauaa acacuggcuc guccauaagg aaugguucca cgacauuccu     1260

cugcccuggc acgcuggggc cgauacagga acgccucauu ggaacaauaa agaggcccug     1320

guggaauuua aggacgcuca cgccaagaga cagaccguug ugguccucgg cagccaggag     1380

ggggcggugc auacagcucu ggccggcgcc cuugaggcug aaauggaugg agccaaaggc     1440

aagcuguucu ccgggcaccu caaaugccgc cugaagaugg acaaacuucg gcugaaggga     1500

guuagcuauu cucucuguac cgcugccuuu acauucacca aggugcccgc ggagacgcug     1560

cauggcaccg ucacagugga agugcaguac gccgggaccg acggaccgug caaaaucccu     1620

guucaaaugg cugucgauau gcagacacug acccccgugg gcagacucau cacggccaac     1680

ccugugauua cagaguccac cgaaaacagc aagaugaugc uggagcuuga cccucccuuu     1740

ggggauucuu acaucgucau uggcguggga gacaaaaaga ucacacacca cuggcaucgc     1800

uccggcagca ccauugggaa ggcuuucgag gccaccguuc ggggagcgaa aagaauggcc     1860

gugcugggcg auacagcuug ggacuucggg uccgucggag gcguguuuaa uucucucggg     1920

aagggcaucc accagaucuu cggagccgcu uuuaaaagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucugaucgg aacgcuucuc guuuggcugg gccugaacac caagaauggg     2040

agcauuuccc ucacaugucu ggcccuugga ggcgugauga ucuuccugag caccgccguc     2100

ucugcugaug uggggugcuc cguugacuuu agcaaa                               2136


<210>  2297
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long CDS opt

<400>  2297
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

gugggacugc uccugacaac ggccauggcg gcugaaauca cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauua gcuucgccac aacccuggga      180

gucaauaaau gccacguuca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaaggggug gagcccgacg auguggacug uuggugcaac      300

accacaucua ccugggucgu guacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cgguuacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauca aaguggaaaa uuggauuuuu      480

agaaaccccg gcuucacgcu ggucgccgug gcuaucgccu ggcuccuggg gagcucuaca      540

ucccagaagg uuauuuaccu ugugaugauc cugcucauug cuccugccua uagcauccgg      600

ugcauuggag ucucuaaucg cgauuuugug gagggcaugu ccgggggaac cuggguggac      660

gucguucugg aacacggcgg gugugugaca gugauggcgc aggauaaacc caccgucgac      720

aucgagcuug ugaccacaac gguuagcaac auggccgagg ugagaucuua cugcuacgaa      780

gccuccauca gcgauauggc uuccgacucu cgguguccua cccaaggaga ggcuuaucug      840

gacaagcaga gcgauacaca guacgucugc aaaagaaccc ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cugguuacau gcgccaaguu uaccugcagc      960

aaaaagauga cgggaaaauc uauucagccc gaaaaccucg aguaucggau caugcugucc     1020

gugcacggca gccaacauuc ugggaugauu gucaaugaug aaaacagagc caagguggag     1080

gucacaccua auagcccgcg cgcugaggcg acccuuggag gcuucggcuc ccuggggcuc     1140

gacugugaac cccggaccgg acuggauuuu ucugaccuuu acuaucugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggagugguuc cacgauaucc cucugcccug gcacgccggc     1260

gcugacaccg ggacaccuca uuggaacaau aaagaagccc ugguugaguu uaaggaugcc     1320

cacgcuaaga gacagacggu ggucgugcuc ggaagccagg aaggcgccgu ucauaccgcg     1380

cuggcugggg cccuugaggc cgagauggac ggcgcuaaag gacgccuguu cuccggccac     1440

cucaagugcc ggcugaaaau ggauaaacuu agacugaagg gggugagcua cucucucugu     1500

acagccgcuu uuaccuucac aaaggucccc gccgaaaccc ugcauggaac ggugaccgug     1560

gagguucagu augcgggcac agacgggccg ugcaaggucc cugcccaaau ggcuguggac     1620

augcagaccc ugacacccgu gggacgccuc auuaccgcca acccugucau cacggaaucc     1680

acagagaaca gcaaaaugau gcuggaacuu gauccucccu uuggcgacuc uuacaucgug     1740

auugggguug gcgauaagaa aaucacccac cacuggcauc gguccggaag cacaauuggc     1800

aaggcuuucg aggccaccgu gagaggggcg aaacgcaugg ccguccuggg agacaccgcu     1860

ugggauuucg gcuccguugg gggaguguuu aauucucucg gcaaggggau ccaccagauu     1920

uucggcgccg cuuuuaagag ccuguucgga ggcauguccu gguuuucuca aauccugauc     1980

gggacacuuc ucguguggcu gggacugaac acgaaaaaug gcagcauuuc ccucaccugu     2040

cuggcccuug ggggagucau gaucuuccug agcacagccg ugucugcuga cguuggcugc     2100

uccguggauu uuagcaag                                                   2118


<210>  2298
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long CDS opt

<400>  2298
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aacccuggga      180

gugaauaaau gccacgucca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccgca agaaaggcga agccagacgg      360

agccgcagag cggugacccu uccgucccau ucuacacgga agcugcaaac ccgcagccag      420

acauggcucg aguccagaga guauaccaag caccugauua aaguugaaaa uuggaucuuu      480

cggaaccccg gcuucgcccu gguggcuguc gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaagg ugaucuaccu uguuaugauu cugcucaucg ccccugcgua uagcauucgc      600

ugcaucggag ugucuaauag agauuuuguc gagggcaugu ccgggggaac auggguggac      660

gugguccugg aacauggcgg guguguuacc gugauggccc aggauaaacc cacaguggac      720

aucgagcuug ucaccaccac agugagcaac auggcugagg uucggucuua cugcuacgaa      780

gccuccauua gcgauauggc uuccgacucu agauguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgugugc aaacgcacac ucgucgaccg gggcuggggc      900

aaugggugug gacuguucgg caaggggucc cuggugaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauccagccc gaaaaccucg aguauagaau uaugcugucc     1020

guucacggca gccaacacuc ugggaugauc gugaaugaua cgggauacga aacagacgag     1080

aaccgcgcua aggucgaggu gaccccuaau agcccgcggg cggaagccac ccuuggcggc     1140

uucggguccc ugggacucga uugugagccc agaacaggcc uggacuuuuc ugaucuuuau     1200

uaccugacca ugaacaauaa acauuggcuc guccacaagg aaugguucca ugacauuccu     1260

cugcccuggc acgcuggggc cgauacagga acgccucacu ggaacaauaa agaggcccug     1320

guggaauuua aggacgcuca ugccaagcgc cagaccguug ugguccucgg cagccaggag     1380

ggggcggugc acacagcucu ggccggcgcc cuugaggcug aaauggaugg agccaaaggc     1440

aagcuguucu ccgggcaucu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

guuagcuauu cucucuguac cgcugccuuu acauucacca aggugcccgc ggagacgcug     1560

cacggcaccg ucacagugga agugcaguac gccgggaccg acggaccgug caaaaucccu     1620

guucaaaugg cugucgauau gcagacacug acccccgugg gccgccucau cacggccaac     1680

ccugugauua cagaguccac cgaaaacagc aagaugaugc uggagcuuga cccucccuuu     1740

ggggauucuu acaucgucau uggcguggga gacaaaaaga ucacacauca cuggcaccgg     1800

uccggcagca ccauugggaa ggcuuucgag gccaccguua gaggagcgaa acgcauggcc     1860

gugcugggcg auacagcuug ggacuucggg uccgucggag gcguguuuaa uucucucggg     1920

aagggcaucc aucagaucuu cggagccgcu uuuaaaagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucugaucgg aacgcuucuc guuuggcugg gccugaacac caagaauggg     2040

agcauuuccc ucacaugucu ggcccuugga ggcgugauga ucuuccugag caccgccguc     2100

ucugcugaug uggggugcuc cguugacuuu agcaaa                               2136


<210>  2299
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long CDS opt

<400>  2299
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aacccuggga      180

gugaauaaau gccacgucca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cggugacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauua aaguugaaaa uuggaucuuu      480

agaaaccccg gcuucgcccu gguggcuguc gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaagg ugaucuaccu uguuaugauu cugcucaucg ccccugcgua uagcauucgg      600

ugcaucggag ugucuaaucg cgauuuuguc gagggcaugu ccgggggaac auggguggac      660

gugguccugg aacacggcgg guguguuacc gugauggccc aggauaaacc cacaguggac      720

aucgagcuug ucaccaccac agugagcaac auggcugagg uuagaucuua cugcuacgaa      780

gccuccauua gcgauauggc uuccgacucu cgguguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgugugc aaaagaacac ucgucgaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cuggugaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauccagccc gaaaaccucg aguaucggau uaugcugucc     1020

guucacggca gccaacauuc ugggaugacg gugaaugaua ucggauacga aacagacgag     1080

aacagagcua aggucgaggu gaccccuaau agcccgcgcg cggaagccac ccuuggcggc     1140

uucggguccc ugggacucga uugugagccc cggacaggcc uggacuuuuc ugaucuuuau     1200

uaccugacca ugaacaauaa acacuggcuc guccauaagg aaugguucca cgacauuccu     1260

cugcccuggc acgcuggggc cgauacagga acgccucauu ggaacaauaa agaggcccug     1320

guggaauuua aggacgcuca cgccaagaga cagaccguug ugguccucgg cagccaggag     1380

ggggcggugc auacagcucu ggccggcgcc cuugaggcug aaauggaugg agccaaaggc     1440

aagcuguucu ccgggcaccu caaaugccgc cugaagaugg acaaacuucg gcugaaggga     1500

guuagcuauu cucucuguac cgcugccuuu acauucacca aggugcccgc ggagacgcug     1560

cauggcaccg ucacagugga agugcaguac gccgggaccg acggaccgug caaaaucccu     1620

guucaaaugg cugucgauau gcagacacug acccccgugg gcagacucau cacggccaac     1680

ccugugauua cagaguccac cgaaaacagc aagaugaugc uggagcuuga cccucccuuu     1740

ggggauucuu acaucgucau uggcguggga gacaaaaaga ucacacacca cuggcaucgc     1800

uccggcagca ccauugggaa ggcuuucgag gccaccguuc ggggagcgaa aagaauggcc     1860

gugcugggcg auacagcuug ggacuucggg uccgucggag gcguguuuaa uucucucggg     1920

aagggcaucc accagaucuu cggagccgcu uuuaaaagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucugaucgg aacgcuucuc guuuggcugg gccugaacac caagaauggg     2040

agcauuuccc ucacaugucu ggcccuugga ggcgugauga ucuuccugag caccgccguc     2100

ucugcugaug uggggugcuc cguugacuuu agcaaa                               2136


<210>  2300
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long CDS opt

<400>  2300
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aacccuggga      180

gugaauaaau gccacgucca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cggugacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauua aaguugaaaa uuggaucuuu      480

agaaaccccg gcuucgcccu gguggcuguc gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaagg ugaucuaccu uguuaugauu cugcucaucg ccccugcgua uagcauucgg      600

ugcaucggag ugucuaaucg cgauuuuguc gagggcaugu ccgggggaac auggguggac      660

gugguccugg aacacggcgg guguguuacc gugauggccc aggauaaacc cacaguggac      720

aucgagcuug ucaccaccac agugagcaac auggcugagg uuagaucuua cugcuacgaa      780

gccuccauua gcgauauggc uuccgacucu cgguguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgugugc aaaagaacac ucgucgaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cuggugaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauccagccc gaaaaccucg aguaucggau uaugcugucc     1020

guucacggca gccaacauuc ugggaugauc ggauacgaaa cggaugagaa uagagcuaag     1080

guggagguca caccuaacag cccgcgcgcg gaagccaccc uuggcggcuu cgggucccug     1140

ggacucgacu gugagccccg gaccggccug gauuuuucug accuuuauua ccugacaaug     1200

aauaacaaac acuggcucgu gcauaaggaa ugguuccacg auauuccucu gcccuggcac     1260

gcuggggccg acaccggaac accucauugg aauaacaaag aggcccuggu cgaauuuaag     1320

gaugcucacg ccaagagaca gacggugguu gugcucggca gccaggaggg ggcgguccau     1380

accgcucugg ccggcgcccu ugaggcugaa auggacggag ccaaaggcaa gcuguucucc     1440

gggcaccuca aaugccgccu gaagauggau aaacuucggc ugaagggagu gagcuauucu     1500

cucuguacag cugccuuuac cuucacaaag guucccgcgg agacccugca uggcacggug     1560

accgucgaag ugcaguacgc cgggacagac ggaccgugca aaaucccugu gcaaauggcu     1620

guugacaugc agacccugac acccgucggc agacucauca ccgccaaucc ugugauuacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugauc cucccuuugg ggacucuuac     1740

aucgugauug gcgucggaga uaaaaagauc acccaccacu ggcaucgcuc cggcagcaca     1800

auugggaagg cuuucgaggc caccgugcgg ggagcgaaaa gaauggccgu ucugggcgac     1860

accgcuuggg auuucggguc cgugggaggc gucuuuaacu cucucgggaa gggcauccac     1920

cagaucuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauu     1980

cugaucggaa cacuucucgu guggcugggc cugaauacga agaacgggag cauuucccuc     2040

accugucugg cccuuggagg cguuaugauc uuccugagca cagccguguc ugcugacguc     2100

gggugcuccg uggauuuuag caaa                                            2124


<210>  2301
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long CDS opt

<400>  2301
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

cggggcauuc accagaucuu cggagcugcg uuuaaaagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaag                               2136


<210>  2302
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long CDS opt

<400>  2302
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacgccag cguggggauc       60

gucggacugc uccugaccac agcgauggcu gccgaaguua cgcggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgcuggg gaggccauuu cuuuccccac cacacuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uaccaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

acaaccucca caugggucgu guauggaacc ugucaucaca agaaaggcga ggcccggcgc      360

ucuagacggg cgguuacgcu ucccagccau uccacccgca agcugcaaac aagaucucag      420

accuggcucg agagccggga auacacaaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcccu ggcugccgcu gccauugcgu ggcuccuggg guccagcacc      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcuua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac gugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggccc aggauaagcc uaccguggac      720

auugagcuug ugacaaccac cgucuccaau auggcugaag ugagaagcua cugcuaugag      780

gccucuaucu ccgauauggc cagcgacucc cgguguccca cacaaggaga ggcuuaccug      840

gacaaacagu cugauacgca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggucacau gcgcgaaguu ugccugcucc      960

aagaaaauga ccggaaagag cauccagccu gaaaaucucg aauaccggau uaugcugucu     1020

gugcacggcu cccaacacag cgggaugauc guuaacgaua caggacauga gaccgacgag     1080

aauagagcua aaguggaaau uacgccgaac ucuccccgcg ccgaggccac acuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacca ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgcuggggc cgauacagga acaccccauu ggaauaacaa ggaagcgcug     1320

guggaguuua aagacgcuca cgccaagaga cagaccgucg ugguucucgg cucucaggag     1380

ggggccgugc auacggcucu ggccggcgcu cuugaagccg agauggaugg agcgaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac cgccgcuuuc acauuuacca aaauuccggc cgaaacacug     1560

cauggcaccg ugacgguuga ggugcaguac gcugggaccg acggaccuug caaggucccc     1620

gcccaaaugg cgguggauau gcagacacug accccugugg gccgccucau cacagccaac     1680

cccguuauca ccgaauccac ggagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa ggcuuuugaa gccacaguga gaggagcuaa acgcauggcc     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcucucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcggcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacccuccug auguggcugg gccucaauac aaagaacggg     2040

agcaucuccc ugaugugucu ugcucuggga ggcguucuca uuuuccugag cacggccgug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2303
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long CDS opt

<400>  2303
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gccuggcgcu cgccgcugcc gcuauugccu ggcugcuugg guccagcacg      540

ucucagaaag ucaucuaucu ggugaugauu cuccugaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuucgug gaaggcaugu cugggggaac augggucgac      660

guggugcuug agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcugg ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccuc      840

gacaaacagu cugauaccca guauguuugc aagagaacac ugguggaccg cggcuggggc      900

aacgggugug gacuguuugg caaagggagc cucgugaccu gcgcuaaguu cgcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucugg aauaccggau uaugcuuucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccugggcggc     1140

uuugggagcc ucggacugga uugugaaccu cggaccggcc uugacuucuc cgaucuguau     1200

uaccucacaa ugaauaacaa gcacuggcug guccauaaag agugguuuca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccuc     1320

guggaguuca aagacgcgca cgcuaagaga cagacggucg ugguucuggg cucucaggag     1380

ggggccgugc auaccgcccu ugcuggcgcc cuggaagcug agauggaugg agccaagggc     1440

cgccucagcu ccgggcaccu gaaaugccgg cuuaagaugg acaaacugag acucaaggga     1500

gucagcuauu cucuguguac agcggccuuu accuucacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccuc acaccugugg gccgccugau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uugagcugga ucccccuuuu     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuucgaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccucggcg acaccgccug ggauuuuggg uccgugggag gcgcccugaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uucaagagcc uuuuuggcgg gauguccugg     1980

uucucucaaa uccucauugg aacacugcug auguggcucg gccugaauac gaagaacggg     2040

agcaucuccc uuaugugucu ggcccucgga ggcguucuga uuuuucugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuc agcaaa                               2136


<210>  2304
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long CDS opt

<400>  2304
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cugguaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc aucagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2305
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long CDS opt

<400>  2305
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaaa ucagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguugaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcgca cgcuaagaga cagacggugg ucgugcucgg cucucaggag     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccgugauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcgugcuca ucuuccugag caccgcuguu     2100

ucugccgacg uggggugcuc cgucgauuuu agcaaa                               2136


<210>  2306
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long CDS opt

<400>  2306
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc ccaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caagguccug     1620

gcucaaaugg ccguggauau gcagacccuc acacccgugg gccgccugau caccgcgaac     1680

ccuguuauca cggaauccac agagaauagc aagaugaugc uugagcugga cccucccuuc     1740

ggggauucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccucggcg acaccgccug ggauuucggg uccgugggag gcgcccugaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uuuucggcgg gauguccugg     1980

uuuucucaaa uccucauugg aacacugcug auguggcucg gccugaauac gaagaacggg     2040

agcaucuccc uuaugugucu ggcccucgga ggcguucuga uuuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2307
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long CDS opt

<400>  2307
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuacccugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

acaaccucca cgugggucgu guauggaacc ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacacu ucccagccau uccacccgca agcugcaaac aagaucucag      420

accuggcucg agagccggga auacacgaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcaca      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac cugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggcuc aggauaagcc uaccguggac      720

auugagcuug ugaccacaac ggucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cccaaggaga agccuaccug      840

gacaaacagu cugauacaca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggucacau gcgcuaaguu ugcgugcucc      960

aagaaaauga ccggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

gugcacggcu cccaacacag cgggaugauc guuaacgaua cgggacauga gacagacgag     1080

aauagagcca aaguggaaau uaccccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggacaggcc uggacuuuuc cgaucuuuau     1200

uaccugacca ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauacagga acgccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagaccgucg ugguucucgg cucucaggag     1380

ggggccgugc auacagcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac cgcggccuuc acauuuacca aaauuccggc ugaaacgcug     1560

cauggcaccg ugacaguuga ggugcaguac gccgggaccg acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacacug accccugugg gccgccucau cacggcgaac     1680

cccguuauca cagaauccac cgagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acacagccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacgcuccug auguggcugg gccucaauac caagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag cacagcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2308
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long CDS opt

<400>  2308
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgugguc     2100

ucugcugacg uggggugcuc cguugauuuu agcaaa                               2136


<210>  2309
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long CDS opt

<400>  2309
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacauccg cuugggucgu guauggaacc ugucaucaca agaaaggcga ggcccggcgc      360

ucuagacggg cgguuacgcu ucccagccau uccacccgca agcugcaaac aagaccucag      420

accuggcucg agucucggga auacacaaag caccugaucc gcguggagaa cuggaucuuu      480

agaaaucccg gcuucgcccu ggcugccgcu gccauugcgu ggcuccuggg gagcuccacc      540

agccagaaag ucaucuaucu ugugaugauu cugcucaucg ccccugcuua cucuauucgg      600

ugcaucggag uuuccaaccg cgauuuugug gaaggcauga gcgggggaac gugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggccc aggauaagcc caccguggac      720

auugagcuug ugacaaccac cgucucuaau auggcugaag ugagauccua cugcuaugag      780

gccagcaucu cugauauggc cuccgacagc cgguguccua cacaaggaga ggcuuaccug      840

gacaaacagu ccgauacgca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggucu cuggucacau gcgcgaaguu ugccugcagc      960

aagaaaauga ccggaaaguc cauccagccg gagaaucucg aauaccggau uaugcugagc     1020

gugcacggcu cucaacacuc cgggaugauc guuaacgaua caggacauga gaccgacgag     1080

aauagagcua aaguggaaau uacgcccaac agcccucgcg ccgaggccac acuuggcggc     1140

uucgggucuc ugggacucga uugugaaccc cggaccggcc uggacuuuag cgaucuuuau     1200

uaccugacca ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccu     1260

cugcccuggc acgcuggggc cgauacagga accccgcauu ggaauaacaa ggaagcgcug     1320

guggaguuua aagacgcuca cgccaagaga cagacagucg ugguucucgg cucccaggag     1380

ggggccgugc auacggcucu ggccggcgcu cuugaagccg agauggaugg agcgaagggc     1440

cgccugucua gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucuccuaua gccucuguac cgccgcuuuc acauuuacca aaauuccugc cgaaacacug     1560

cauggcaccg ugacgguuga ggugcaguac gcugggaccg acggacccug caaggucccu     1620

gcccaaaugg cgguggauau gcagacacug acccccgugg gccgccucau cacagccaac     1680

cccguuauca ccgaaucuac ggaaaauucc aagaugaugc uggagcuuga cccuccguuc     1740

ggggauagcu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

ucuggcucca ccaucgggaa ggcuuuugaa gccacaguga gaggagcuaa acgcauggcc     1860

guccugggcg acaccgccug ggauuucggg agcgugggag gcgcucucaa cucccugggg     1920

aaaggcauuc accagaucuu cggagcggcc uuuaagucuc uguucggcgg gaugagcugg     1980

uuuucccaaa uccuuauugg aacccuccug auguggcugg gccucaauac aaagaacggg     2040

ucuaucagcc ugaugugucu ugcucuggga ggcguucuca uuuuccuguc cacggccgug     2100

agcgccgacg ucgggugcuc uguggauuuu uccaaa                               2136


<210>  2310
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long CDS opt

<400>  2310
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucuaaaaagg ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaaacc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcagggaga agccuaccug      840

gacaagcaau cugauaccca guauguuugc aaaagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaggggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aaaaagauga caggaaaaag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccagcacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca agguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa acacuggcuc guccauaagg agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa agaagcccug     1320

guggaguuua aggacgcgca cgcuaagaga caaacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaaaggc     1440

cgccugagcu ccgggcaccu caagugccgg cugaaaaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa agauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucagaugg ccguggauau gcaaacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aaaaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gaaaagaaaa uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa ggccuuugag gcuaccguga gaggagccaa acgcauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aagggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaga uccuuauugg aacacuccug auguggcugg gccucaauac gaaaaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaag                               2136


<210>  2311
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long CDS opt

<400>  2311
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

cggcagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cucuauucgc      600

ugcaucggag uuuccaacag agauuuugug gaaggcauga gcgggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucucuaau auggccgaag ugcgguccua cugcuaugag      780

gcuagcaucu cugauauggc cuccgacagc agauguccca cgcaaggaga agccuaccug      840

gacaaacagu ccgauaccca guauguuugc aagcgcacac ucguggaccg gggcuggggc      900

aacgggugug gacuguucgg caaagggucu cuggugaccu gcgcuaaguu ugcgugcagc      960

aagaaaauga caggaaaguc cauccagccu gagaaucucg aauacagaau uaugcugagc     1020

guccacggcu cucaacacuc cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aaucgcgcca aaguggaaau uacaccgaac agcccccggg cugaggccac ccuuggcggc     1140

uucgggucuc ugggacucga uugugaaccu agaaccggcc uggacuuuag cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagcgc cagacggucg ugguucucgg cucccaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cggcugucua gcgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gucuccuaua gccucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccggcucau caccgcgaac     1680

cccguuauca cggaaucuac agaaaauucc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacagcu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcauaga     1800

ucuggcucca caaucgggaa agccuuugag gcuaccgugc gcggagccaa gcggauggcu     1860

guccugggcg acaccgccug ggauuucggg agcgugggag gcgcccucaa cucccugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagucuc uguucggcgg gaugagcugg     1980

uuuucccaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

ucuaucagcc ugaugugucu ugcccuggga ggcguucuca uuuuccuguc caccgcugug     2100

agcgccgacg ucgggugcuc uguggauuuu uccaaa                               2136


<210>  2312
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long CDS opt

<400>  2312
agaaucauua acgcccggaa ggagaaaaag cgcagaagcg cugacaccuc cgugggcauc       60

gucgggcugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg aucugccuac      120

uauauguacc uugauagaaa ugacgccggc gaggcuauua gcuuccccac aacccugggg      180

augaacaaau gcuauaucca gauuauggau cucggacaca ugugugacgc cacaaugucc      240

uacgaaugcc cuaugcugga ugagggcgug gaaccggacg auguggacug uuggugcaau      300

accacaucua ccugggucgu guaugggacg ugucaucaca agaaaggaga ggcucggcgc      360

agcagacggg ccguuacccu ucccucccau ucuacacgca agcugcaaac cagaagccag      420

acauggcucg agucccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg cagcucuacg      540

ucccagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cagcauucgg      600

ugcaucgggg uuucuaaccg cgauuuugug gaaggaaugu ccggcgggac augggucgac      660

guggugcugg agcauggagg cugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucagcaau auggccgaag ugagaucuua cugcuaugag      780

gcuuccauca gcgauauggc cuccgacucu cgguguccca cgcaagggga agccuaccug      840

gacaaacaga gcgauaccca guauguuugc aagagaacac ucguggaccg cggauggggc      900

aacggcugug ggcuguucgg aaaaggcucc cuggugaccu gcgcuaaguu ugcgugcagc      960

aagaaaauga cagggaaguc uauccagccu gagaaucucg aauaccggau uaugcugucc     1020

gugcacggaa gccaacacuc uggcaugauc guuaacgaua ccgggcauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac agcccccgcg cugaggccac ccuuggaggc     1140

uucggcuccc uggggcucga uugugaaccu cggaccggac uggacuuuuc ugaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggcgc ugauaccggg acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg aagccaggag     1380

ggcgccgugc auaccgcccu ggcuggggcc cuugaagcug agauggaugg cgccaaggga     1440

cgccugucca gcggccaccu caaaugccgg cugaagaugg acaaacuuag acugaagggg     1500

gucucuuauu cccucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggaacgg ugaccguuga ggugcaguac gccggcacag acgggccuug caaggucccc     1620

gcucaaaugg ccguggauac ccagacacug accccugugg gacgccucau cacggcgaac     1680

cccguuauca cagaaagcac cgaaaauucu aagaugaugc uggagcuuga ucccccuuuc     1740

ggcgacuccu acauugucau cggggugggc gagaaaaaga uuacacacca cuggcaucgg     1800

agcggaucca ccaucggcaa agccuuugag gcuaccguga gaggggccaa gcgcauggcu     1860

guccugggag acacagccug ggauuucggc ucugucgggg gagcccucaa cagccugggc     1920

aaagggauuc accagaucuu cggcgcugcg uuuaaguccc uguucggagg caugucuugg     1980

uuuagccaaa uccuuauugg gacgcuccug auguggcugg gacucaauac caagaacggc     2040

uccaucagcc ugaugugucu ugcccucggg ggaguucuga uuuuccuguc uacagcugug     2100

uccgccgacg ucggcugcag cguggauuuu ucuaaa                               2136


<210>  2313
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long CDS opt

<400>  2313
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccaa uguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cagcgccuac      120

uauauguacc uugauagaaa cgacgccggg gaggcuauuu ccuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugucu      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaagca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

uccagacggg ccguuacccu ucccucucau agcacacgca agcugcaaac cagaucccag      420

acauggcucg agucucggga auacaccaag caccugaucc gcguggagaa uuggaucuuu      480

agaaacccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg gagcuccacg      540

agccagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cucuauucgg      600

ugcaucggag uuuccaaucg cgauuuugug gaaggcauga gcgggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucucuaac auggccgaag ugagauccua cugcuaugag      780

gcuagcaucu cugauauggc cuccgacagc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu ccgauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucu cuggugaccu gcgcuaaguu ugcgugcagc      960

aagaaaauga caggaaaguc cauccagccu gagaaccucg aauaccggau uaugcugagc     1020

guccacggcu cucaacacuc cgggaugauc guuaaugaua ccggacauga gacggacgag     1080

aacagagcca aaguggaaau uacaccgaau agcccccgcg cugaggccac ccuuggcggc     1140

uucgggucuc ugggacucga uugugaaccu cggaccggcc uggacuuuag cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaacaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucccaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugucua gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucuccuaua gccucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaau     1680

cccguuauca cggaaucuac agagaacucc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacagcu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

ucuggcucca caaucgggaa agccuuugaa gcuaccguga gaggagcccg ccggauggcu     1860

guccugggcg acaccgccug ggauuucggg agcgugggag gcgcccucaa uucccugggg     1920

aagggcauuc accagaucuu cggagcugcg uuuaaaucuc uguucggcgg gaugagcugg     1980

uuuucccaaa uccuuauugg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

ucuaucagcc ugaugugucu ugcccuggga ggcguucuca uuuuccuguc caccgcugug     2100

agcgccgacg ucgggugcuc uguggauuuu uccaag                               2136


<210>  2314
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long CDS opt

<400>  2314
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugaaaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugagguua cccggcgcgg cuccgccuac      120

uauauguacc uugacagaaa ugaugccggg gaagcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggac cucggccaca ugugugaugc cacaaugagc      240

uacgagugcc cuaugcugga cgaaggggug gagccggaug acguggauug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg aaagccggga guacaccaag caccugaucc gcguggaaaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgacuuugug gagggcaugu cugggggaac augggucgau      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggacaagcc uacaguggau      720

auugaacuug ugaccaccac agucuccaau auggccgagg ugagaagcua cugcuaugag      780

gcuucuaucu ccgacauggc cagcgauucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugacaccca guauguuugc aagagaacac ucguggaucg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gaaaaucucg aguaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaca ccggacauga gacggaugaa     1080

aauagagcca aaguggagau uacaccgaac ucuccccgcg cugaagccac ccuuggcggc     1140

uucgggagcc ugggacucga cugugagccu cggaccggcc uggauuuuuc cgaccuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag aaugguucca cgauaucccc     1260

cugccuuggc acgccggggc ugacaccgga acaccccauu ggaauaacaa ggaggcccug     1320

guggaguuua aagaugcgca cgcuaagaga cagacggucg ugguucucgg cucucaggaa     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaggcug aaauggacgg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg auaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugagacccug     1560

cauggcacgg ugaccguuga agugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggacau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaguccac agagaauagc aagaugaugc uggaacuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg auaccgccug ggacuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgaug ucgggugcuc cguggacuuu agcaaa                               2136


<210>  2315
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long CDS opt

<400>  2315
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cucccugcgg      600

ugcauuggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcuug agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

aucgagcugg ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuauuu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccuc      840

gacaaacagu cugauaccca guauguuugc aagagaacac ugguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cucgugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucugg aauaccggau caugcuuucu     1020

guccacggcu cccaacacag cgggaugauu guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau cacaccgaac ucuccccgcg cugaggccac ccugggcggc     1140

uucgggagcc ucggacugga uugugaaccu cggaccggcc uugacuuuuc cgaucuguau     1200

uaccucacaa ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccuc     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucuggg cucucaggag     1380

ggggccgugc auaccgcccu ugcuggcgcc cuggaagcug agauggaugg agccaagggc     1440

cgccucagcu ccgggcaccu gaaaugccgg cuuaagaugg acaaacugag acucaaggga     1500

gucagcuauu cucuguguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccuc acaccugugg gccgccugau uaccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uugagcugga ucccccuuuc     1740

ggggacucuu acaucgucau uggcguggga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccucggcg acaccgccug ggauuucggg uccgugggag gcgcccugaa cucucugggg     1920

aaaggcaucc accagauuuu cggagcugcg uuuaagagcc uuuucggcgg gauguccugg     1980

uuuucucaaa uccucaucgg aacacugcug auguggcucg gccugaauac gaagaacggg     2040

agcauuuccc uuaugugucu ggcccucgga ggcguucuga ucuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2316
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long CDS opt

<400>  2316
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccaa uguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cagcgccuac      120

uauauguacc uugauagaaa cgacgccggg gaggcuauuu ccuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugucu      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaagca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

uccagacggg ccguuacccu ucccucucau agcacacgca agcugcaaac cagaucccag      420

acauggcucg agucucggga auacaccaag caccugaucc gcguggagaa uuggauguuu      480

agaaacccug gcuucgcgcu ggccgcugcc gcuaucgccu ggcuccuggg gagcuccacg      540

agccagaaag ucauuuaucu ugugaugauc cugcucauug cgcccgccua cucuauccgg      600

ugcauuggag uuuccaaucg cgauuuugug gaaggcauga gcgggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

aucgagcuug ugaccaccac agucucuaac auggccgaag ugagauccua cugcuaugag      780

gcuagcauuu cugauauggc cuccgacagc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu ccgauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucu cuggugaccu gcgcuaaguu ugcgugcagc      960

aagaaaauga caggaaaguc cauccagccu gagaaccucg aauaccggau caugcugagc     1020

guccacggcu cucaacacuc cgggaugauu guuaaugaua ccggacauga gacggacgag     1080

aacagagcca aaguggaaau cacaccgaau agcccccgcg cugaggccac ccuuggcggc     1140

uucgggucuc ugggacucga uugugaaccu cggaccggcc uggacuuuag cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaacaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucccaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugucua gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucuccuaua gccucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau uaccgcgaau     1680

cccguuauca cggaaucuac agagaacucc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacagcu acaucgucau uggcguggga gagaaaaaga ucacccacca cuggcaucgg     1800

ucuggcucca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg agcgugggag gcgcccucaa uucccugggg     1920

aaaggcaucc accagauuuu cggagcugcg uuuaagucuc uguucggcgg gaugagcugg     1980

uuuucccaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

ucuauuagcc ugaugugucu ugcccuggga ggcguucuca ucuuccuguc caccgcugug     2100

agcgccgacg ucgggugcuc uguggauuuu uccaaa                               2136


<210>  2317
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long CDS opt

<400>  2317
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggauccug      480

agaaauccug gcuuugcgcu cgccgcugcc gcuauugccu ggcugcuugg guccagcacg      540

ucucagaaag ucaucuaucu ggugaugauu cuccugaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuucgug gaaggcaugu cugggggaac augggucgac      660

guggugcuug agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcugg ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccuc      840

gacaaacagu cugauaccca guauguuugc aagagaacac ugguggaccg cggcuggggc      900

aacgggugug gacuguuugg caaagggagc cucgugaccu gcgcuaaguu cgcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucugg aauaccggau uaugcuuucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccugggcggc     1140

uuugggagcc ucggacugga uugugaaccu cggaccggcc uugacuucuc cgaucuguau     1200

uaccucacaa ugaauaacaa gcacuggcug guccauaaag agugguuuca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccuc     1320

guggaguuca aagacgcgca cgcuaagaga cagacggucg ugguucuggg cucucaggag     1380

ggggccgugc auaccgcccu ugcuggcgcc cuggaagcug agauggaugg agccaagggc     1440

cgccucagcu ccgggcaccu gaaaugccgg cuuaagaugg acaaacugag acucaaggga     1500

gucagcuauu cucuguguac agcggccuuu accuucacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccuc acaccugugg gccgccugau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uugagcugga ucccccuuuu     1740

ggggacucuu acauugucau cggcauugga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuucgaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcucggcg acaccgccug ggauuuuggg uccgucggag gcgcccugaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uucaagagcc uuuuuggcgg gauguccugg     1980

uucucucaaa uucucaucgg aacacugcug auguggcucg gccugaauac gaagaacggg     2040

agcauuuccc uuaugugucu ggcccucgga ggcgugcuga ucuuucugag caccgcuguu     2100

ucugccgacg uggggugcuc cgucgauuuc agcaaa                               2136


<210>  2318
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long CDS opt

<400>  2318
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug uguccaccac cgucagcaau auggccgaag ugagaucuua cugcuaugag      780

gcuacaaucu ccgauaucgc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggccg ucgugcucgg cucucaggag     1380

ggggccguuc auaccgcucu ggccggcgcu cuugaagccg agauggaugg agcgaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agccgcuuuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cggucgauau gcagacccug acaccugugg gccgccucau caccgccaac     1680

cccgugauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa ggcuuuugaa gccaccguga gaggagcuaa acgcauggcc     1860

gugcugggcg acaccgccug ggauuucggg uccgucggag gcgcucucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcucuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugccgacg uggggugcuc cgucgauuuu agcaaa                               2136


<210>  2319
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long CDS opt

<400>  2319
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cacccaggag     1380

ggggccgugc auacagcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugucua gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucuccuaua gccucuguac cgcggccuuc acauuuacca aaauuccggc ugaaacgcug     1560

cauggcaccg ugacaguuga ggugcaguac gccgggaccg acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacacug accccugugg gccgccucau cacggcgaac     1680

cccguuauca cagaaucuac cgaaaauucc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacagcu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

ucuggcucca ccaucgggaa agccuuugag gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acacagccug ggauuucggg agcgugggag gcgcccucaa cucccugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagucuc uguucggcgg gaugagcugg     1980

uuuucccaaa uccuuauugg aacgcuccug auguggcugg gccucaauac caagaacggg     2040

ucuaucagcc ugaugugucu ugcccuggga ggcguucuca uuuuccuguc cacagcugug     2100

agcgccgacg ucgggugcuc uguggauuuu uccaaa                               2136


<210>  2320
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long CDS opt

<400>  2320
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga gcugcaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccuc acaccugugg gccgccugau caccgcgaac     1680

cccgugauca cggaauccac agagaauagc aagaugaugc uugagcugga ucccccuuuc     1740

ggggacucuu acauuguuau cggcgucgga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcucggcg acaccgccug ggauuucggg uccgucggag gcgcccugaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uuuucggcgg gauguccugg     1980

uuuucucaaa uccucauugg aacacugcug auguggcucg gccugaauac gaagaacggg     2040

agcaucuccc uuaugugucu ggcccucgga ggcgugcuga uuuuccugag caccgcuguu     2100

ucugccgacg uggggugcuc cgucgauuuu agcaaa                               2136


<210>  2321
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long CDS opt

<400>  2321
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugaaaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugagguua cccggcgcgg cuccgccuac      120

uauauguacc uugacagaaa ugaugccggg gaagcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggac cucggccaca ugugugaugc cacaaugagc      240

uacgagugcc cuaugcugga cgaaggggug gagccggaug acguggauug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg aaagccggga guacaccaag caccugaucc gcaucgaaaa cuggauuuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuaucgccu ggcuccuggg guccagcacg      540

ucucagaaag ugauuuaucu ugucaugauc cugcucauug cgcccgccua cuccauccgg      600

ugcauuggag ugagcaaccg cgacuuuguu gagggcaugu cugggggaac auggguggau      660

gucgugcugg agcauggcgg gugugugacc gucauggcuc aggacaagcc uacaguugau      720

aucgaacuug ugaccaccac aguguccaau auggccgagg ucagaagcua cugcuaugag      780

gcuucuaucu ccgacauggc cagcgauucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugacaccca guaugugugc aagagaacac ucguugaucg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gaaaaucucg aguaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaca ccggacauga gacggaugaa     1080

aauagagcca aaguugagau cacaccgaac ucuccccgcg cugaagccac ccuuggcggc     1140

uucgggagcc ugggacucga cugugagccu cggaccggcc uggauuuuuc cgaccuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag aaugguucca cgauauuccc     1260

cugccuuggc acgccggggc ugacaccgga acaccccauu ggaauaacaa ggaggcccug     1320

gucgaguuua aagaugcgca cgcuaagaga cagacggugg ucgugcucgg cucucaggaa     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaggcug aaauggacgg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg auaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugagacccug     1560

cauggcacgg ucaccgugga aguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgacau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccgugauua cggaguccac agagaauagc aagaugaugc uggaacuuga ucccccuuuc     1740

ggggacucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg auaccgccug ggacuucggg uccgucggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcgugcuca ucuuccugag caccgcuguu     2100

ucugccgaug uggggugcuc cgucgacuuu agcaaa                               2136


<210>  2322
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long CDS opt

<400>  2322
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaauccau ugagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucgucau uggcguggga gagaaaaaga ucacacacca cuggcaucgg     1800

uccggcagca ccauugggaa agccuuugaa gcuacaguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacccuccug auguggcugg gccucaauac aaagaacggg     2040

agcauuuccc ugaugugucu ugcccuggga ggcguucuca ucuuccugag cacggcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2323
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long CDS opt

<400>  2323
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agucaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaauuccggc cgaaacccug     1560

cauggcacgg uuaccgugga gguccaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cuguggauau gcagacccug acaccuguug gccgccucau caccgccaac     1680

cccgucauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugugau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agcguuugaa gccaccguca gaggagcuaa gcgcauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccguuggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccu ucaugugucu ggcgcuugga ggcgugcuga uuuuucucag caccgccguc     2100

ucugcugacg uggggugcuc cguugauuuc agcaaa                               2136


<210>  2324
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long CDS opt

<400>  2324
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

aaacugagcu ccgggcaccu caagugccgc cugaaaaugg acaaacuucg gcugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa agauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gcagacucau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aaaaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gaaaagaaaa uuacccacca cuggcaucgc     1800

uccggcagca caaucgggaa ggccuuugag gcuaccgugc ggggagccaa aagaauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aagggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaaaaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaag                               2136


<210>  2325
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long CDS opt

<400>  2325
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagaggcgca cgcuaagaga cagacggucg ugguucucgg cucucaggaa     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaggcug aaauggacgg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg auaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugagacccug     1560

cauggcacgg ugaccguuga agugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggacau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaguccac agagaauagc aagaugaugc uggaacuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg auaccgccug ggacuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgaug ucgggugcuc cguggacuuu agcaaa                               2136


<210>  2326
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long CDS opt

<400>  2326
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccggggcug acggaccuug caaggucccc     1620

gcccaaaugg cgguggauau gcagacacug accccugugg gccgccucau cacagccaac     1680

cccguuauca ccgaauccac ggagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa ggcuuuugaa gccacaguga gaggagcuaa acgcauggcc     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcucucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcggcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacccuccug auguggcugg gccucaauac aaagaacggg     2040

agcaucuccc ugaugugucu ugcucuggga ggcguucuca uuuuccugag cacggccgug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2327
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long CDS opt

<400>  2327
agaaucauua acgccggcaa ggagaaaaag cggcgcgggg cugacaccaa ugugggaauc       60

gucggccugc uccugacaac ggccauggcg gcugaaguua ccagacgggg gagcgccuac      120

uauauguacc uugaucgcaa cgacgccgga gaggcuauuu ccuuccccac aacccugggc      180

augaauaaau gcuauaucca gauuauggau cucgggcaca ugugugacgc cacaaugucu      240

uacgaaugcc cuaugcugga ugagggagug gaaccggacg auguggacug uuggugcaac      300

accacaagca ccugggucgu guauggcacg ugucaucaca agaaaggcga ggcuagacgg      360

ucccgcagag ccguuacccu ucccucucau agcacacgga agcugcaaac ccgcucccag      420

acauggcucg agucuagaga auacaccaag caccugaucc ggguggagaa uuggaucuuu      480

cgcaacccug gguucgcgcu ggccgcugcc gcuauugccu ggcuccuggg aagcuccacg      540

agccagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cucuauuaga      600

ugcaucggcg uuuccaaucg ggauuuugug gaagggauga gcggaggcac augggucgac      660

guggugcugg agcauggggg augugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucucuaac auggccgaag ugcgcuccua cugcuaugag      780

gcuagcaucu cugauauggc cuccgacagc agauguccca cgcaaggcga agccuaccug      840

gacaaacagu ccgauaccca guauguuugc aagcggacac ucguggacag aggcuggggg      900

aauggaugug gccuguucgg gaaaggaucu cuggugaccu gcgcuaaguu ugcgugcagc      960

aagaaaauga caggcaaguc cauccagccu gagaaccucg aauaccgcau uaugcugagc     1020

guccacgggu cucaacacuc cggaaugauc guuaaugaua ccggccauga gacggacgag     1080

aaccgggcca aaguggaaau uacaccgaau agccccagag cugaggccac ccuuggcggg     1140

uucggaucuc ugggccucga uugugaaccu cgcaccgggc uggacuuuag cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggagc ugauaccggc acaccccauu ggaacaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagcgg cagacggucg ugguucucgg gucccaggag     1380

ggcgccgugc auaccgcccu ggcuggagcc cuugaagcug agauggaugg cgccaagggg     1440

agacugucua gcggacaccu caaaugccgc cugaagaugg acaaacuucg gcugaagggc     1500

gucuccuaua gccucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

caugggacgg ugaccguuga ggugcaguac gccggaacag acggcccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg ggagacucau caccgcgaau     1680

cccguuauca cggaaucuac agagaacucc aagaugaugc uggagcuuga ucccccuuuc     1740

ggcgacagcu acauugucau cggagugggc gagaaaaaga uuacccacca cuggcaucgc     1800

ucugggucca caaucggaaa agccuuugaa gcuaccgugc ggggcgccag acgcauggcu     1860

guccuggggg acaccgccug ggauuucgga agcgugggcg gggcccucaa uucccugggc     1920

aagggcauuc accagaucuu cggagcugcg uuuaaaucuc uguucggggg aaugagcugg     1980

uuuucccaaa uccuuauugg cacacuccug auguggcugg ggcucaacac gaagaaugga     2040

ucuaucagcc ugaugugucu ugcccugggc gggguucuca uuuuccuguc caccgcugug     2100

agcgccgacg ucggcugcuc uguggauuuu uccaag                               2136


<210>  2328
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long CDS opt

<400>  2328
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugaaaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugagguua cccggcgcgg cuccgccuac      120

uauauguacc uugacagaaa agaugccggg gaagcuauuu cuuuccccac aacccuggga      180

augaauaagu gcuauaucca gauuauggac cucggccaca ugugugaugc cacaaugagc      240

uacgagugcc cuaugcugga cgaaggggug gagccggaug acguggauug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca aaaagggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg aaagccggga guacaccaaa caccugaucc gcguggaaaa uuggaucuuu      480

agaaacccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaagg ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaucg cgacuuugug gagggcaugu cugggggaac augggucgau      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggacaaacc uacaguggau      720

auugaacuug ugaccaccac agucuccaac auggccgagg ugagaagcua cugcuaugag      780

gcuucuaucu ccgacauggc cagcgauucc cgguguccca cgcaaggaga agccuaccug      840

gacaagcagu cugacaccca guauguuugc aaaagaacac ucguggaucg cggcuggggc      900

aaugggugug gacuguucgg caaggggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aaaaagauga caggaaaaag cauccagccu gaaaaccucg aguaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaaugaca ccggacauga gacggaugaa     1080

aacagagcca agguggagau uacacccaau ucuccgcgcg cugaagccac ccuuggcggc     1140

uucgggagcc ugggacucga cugugagccu cggaccggcc uggauuuuuc cgaccuuuau     1200

uaccugacaa ugaacaauaa acacuggcuc guccauaagg aaugguucca cgauaucccc     1260

cugccuuggc acgccggggc ugacaccgga acaccccauu ggaacaauaa agaggcccug     1320

guggaguuua aggaugcgca cgcuaagaga cagacggucg ugguucucgg cucucaggaa     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaggcug aaauggacgg agccaaaggc     1440

cgccugagcu ccgggcaccu caagugccgg cugaaaaugg auaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa agauuccggc ugagacccug     1560

cauggcacgg ugaccguuga agugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggacau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaguccac agagaacagc aaaaugaugc uggaacuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaagaaaa uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa ggccuuugaa gcuaccguga gaggagccaa acgcauggcu     1860

guccugggcg auaccgccug ggacuucggg uccgugggag gcgcccucaa uucucugggg     1920

aagggcauuc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaacac gaaaaauggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgaug ucgggugcuc cguggacuuu agcaag                               2136


<210>  2329
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long CDS opt

<400>  2329
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggu uagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucgucgaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guucacggcu cccaacacag cgggaugauc gugaacgaua ccggacauga gacggacgag     1080

aauagagcga aagucgaaau uacaccgaac ucuccccgcg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaauaacaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagaga cagacggugg uugugcucgg cucucaggag     1380

ggggcugucc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaauuccggc cgaaacccug     1560

cauggcacgg uuaccgugga gguccaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cuguggauau gcagacccug acaccuguug gccgccucau caccgccaac     1680

cccgucauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugugau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agcguuugaa gccaccguca gaggagcuaa gcgcauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccguuggag gcgcccucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcgcuggga ggcgugcuca uuuuccugag caccgccguc     2100

ucugcugacg uggggugcuc cguugauuuu agcaaa                               2136


<210>  2330
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long CDS opt

<400>  2330
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccaa uguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cagcgccuac      120

uauauguacc uugauagaaa cgacgccggg gaggcuauuu ccuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugucu      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

augaccagca caugggucgu guauggaacc ugucaucaca agaaaggcga ggcucggcgc      360

uccagacggg ccguuacgcu ucccucucau agcacccgca agcugcaaac aagaucccag      420

accuggcucg agucucggga auacacaaag caccugaucc gcguggagaa uuggaucuuu      480

agaaacccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg gagcuccacc      540

agccagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cucuauucgg      600

ugcaucggag uuuccaaucg cgauuuugug gaaggcauga gcgggggaac gugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggcuc aggauaagcc uaccguggac      720

auugagcuug ugacaaccac cgucucuaac auggccgaag ugagauccua cugcuaugag      780

gcuagcaucu cugauauggc cuccgacagc cgguguccca cacaaggaga agccuaccug      840

gacaaacagu ccgauacgca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucu cuggucacau gcgcuaaguu ugcgugcagc      960

aagaaaauga ccggaaaguc cauccagccu gagaaccucg aauaccggau uaugcugagc     1020

gugcacggcu cucaacacuc cgggaugauc guuaaugaua caggacauga gaccgacgag     1080

aacagagcca aaguggaaau uacgccgaau agcccccgcg cugaggccac acuuggcggc     1140

uucgggucuc ugggacucga uugugaaccu cggaccggcc uggacuuuag cgaucuuuau     1200

uaccugacca ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauacagga acaccccauu ggaacaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagaccgucg ugguucucgg cucccaggag     1380

ggggccgugc auacggcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugucua gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucuccuaua gccucuguac cgcggccuuc acauuuacca aaauuccggc ugaaacacug     1560

cauggcaccg ugacgguuga ggugcaguac gccgggaccg acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacacug accccugugg gccgccucau cacagcgaau     1680

cccguuauca ccgaaucuac ggagaacucc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacagcu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

ucuggcucca ccaucgggaa agccuuugaa gcuacaguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg agcgugggag gcgcccucaa uucccugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagucuc uguucggcgg gaugagcugg     1980

uuuucccaaa uccuuauugg aacccuccug auguggcugg gccucaacac aaagaauggg     2040

ucuaucagcc ugaugugucu ugcccuggga ggcguucuca uuuuccuguc cacggcugug     2100

agcgccgacg ucgggugcuc uguggauuuu uccaaa                               2136


<210>  2331
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long CDS opt

<400>  2331
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau uacaccgaac ucuccccgcg cugaggccgc ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacca ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgcuggggc cgauacagga acaccccauu ggaauaacaa ggaagcgcug     1320

guggaguuua aagacgcuca cgccaagaga cagaccgucg ugguucucgg cucucaggag     1380

ggggccgugc auacggcucu ggccggcgcu cuugaagccg agauggaugg agcgaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac cgccgcuuuc acauuuacca aaauuccggc cgaaacacug     1560

cauggcaccg ugacgguuga ggugcaguac gcugggaccg acggaccuug caaggucccc     1620

gcccaaaugg cgguggauau gcagacacug accccugugg gccgccucau cacagccaac     1680

cccguuauca ccgaauccac ggagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa ggcuuuugaa gccacaguga gaggagcuaa acgcauggcc     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcucucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcggcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa cccuuaucgg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcauuuccc ugaugugucu ugcucuggga ggcguucuca ucuuccugag caccgccgug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2332
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long CDS opt

<400>  2332
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugaaaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugagguua cccggcgcgg cuccgccuac      120

uauauguacc uugacagaaa ugaugccggg gaagcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggac cucggccaca ugugugaugc cacaaugagc      240

uacgagugcc ccaugcugga cgaaggggug gagccggaug acguggauug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg aaagccggga guacaccaag caccugaucc gcguggaaaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcuggccua cuccauucgg      600

ugcaucggag uuagcaaccg cgacuuugug gagggcaugu cugggggaac augggucgau      660

guggugcuug agcauggcgg gugugucacc guuauggcuc aggacaagcc cacaguggau      720

auugaacugg ugaccaccac agucuccaau auggccgagg ugagaagcua cugcuaugag      780

gcuucuaucu ccgacauggc cagcgauucc cgguguccua cgcaaggaga agccuaccuc      840

gacaaacagu cugacaccca guauguuugc aagagaacac ugguggaucg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cucgugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccagccc gaaaaucugg aguaccggau uaugcuuucu     1020

guccacggcu cccaacacag cgggaugauc guuaacgaca ccggacauga gacggaugaa     1080

aauagagcca aaguggagau uacaccuaac ucuccgcgcg cugaagccac ccugggcggc     1140

uucgggagcc ucggacugga cugugagccc cggaccggcc uugauuuuuc cgaccuguau     1200

uaccucacaa ugaauaacaa gcacuggcug guccauaaag aaugguucca cgauaucccu     1260

cugcccuggc acgccggggc ugacaccgga acaccucauu ggaauaacaa ggaggcccuc     1320

guggaguuua aagaugcgca cgcuaagaga cagacggucg ugguucuggg cucucaggaa     1380

ggggccgugc auaccgcccu ugcuggcgcc cuggaggcug aaauggacgg agccaagggc     1440

cgccucagcu ccgggcaccu gaaaugccgg cuuaagaugg auaaacugag acucaaggga     1500

gucagcuauu cucuguguac agcggccuuc accuuuacaa aaauucccgc ugagacccug     1560

cauggcacgg ugaccguuga agugcaguac gccgggacag acggaccgug caaggucccu     1620

gcucaaaugg ccguggacau gcagacccuc acacccgugg gccgccugau caccgcgaac     1680

ccuguuauca cggaguccac agagaauagc aagaugaugc uugaacugga uccucccuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcagca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccucggcg auaccgccug ggacuucggg uccgugggag gcgcccugaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaagagcc uuuucggcgg gauguccugg     1980

uuuucucaaa uccucauugg aacacugcug auguggcucg gccugaauac gaagaacggg     2040

agcaucuccc uuaugugucu ggcccucgga ggcguucuga uuuuccugag caccgcugug     2100

ucugccgaug ucgggugcuc cguggacuuu agcaaa                               2136


<210>  2333
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long CDS opt

<400>  2333
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggaa uuagcaaccg cgauuuuguu gaaggcaugu cugggggaac auggguggac      660

gucgugcugg agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaau auggccgaag ucagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguugaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggauuc ugggacucga uugugaaccu cggaccggcc uggacuuuag cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcgca cgcuaagaga cagacggugg ucgugcucgg cucccaggag     1380

ggggccguuc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugucua gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

guguccuaua gccucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccgucgauau gcagacccug acaccugugg gccgccucau uaccgcgaac     1680

cccgugauca cggaaucuac agaaaauucc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacagcu acauuguuau cggcgucgga gagaaaaaga uuacccacca cuggcaucgg     1800

ucuggcucca caaucgggaa agccuuugag gcuaccguga gaggagccaa gcgcauggcu     1860

gugcugggcg acaccgccug ggauuucggg agcgucggag gcgcccucaa cucccugggg     1920

aaaggcaucc accagauuuu cggagcugcg uuuaagucuc uguucggcgg gaugagcugg     1980

uuuucccaaa uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

ucuaucagcc ugaugugucu ugcccuggga ggcgugcuca uuuuccuguc caccgcuguu     2100

agcgccgacg uggggugcuc ugucgauuuu uccaaa                               2136


<210>  2334
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long CDS opt

<400>  2334
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca caugugacgc caccaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

acaaccucca cgugggucgu guauggaacc ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacacu ucccagccau uccauccgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggauuuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuaucgccu ggcuccuggg guccagcacg      540

ucucagaaag ucauuuaucu ugugaugauc cugcucauug cgcccgccua cuccauccgg      600

ugcauuggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

aucgagcuug ugaccaccac agucuccaau auggccgaag ugagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauucagccu gagaaucucg aauaccggau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu guuaacgaua ccggacauga gacggacgag     1080

aauagagcca aaguggaaau cacaccgaac ucuccccgcg cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaaucccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauua cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acaucgucau uggcguggga gagaaaaaga ucacccacca cuggcaucgg     1800

uccggcagca caauugggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcaucc accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucuuaucgg aacacuccug auguggcugg gccucaaugc caagaacggg     2040

agcauuuccc ugaugugucu ugcucuggga ggcguucuca ucuuccugag cacggccgug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2335
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long CDS opt

<400>  2335
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucaacgg      360

ucucgcagag ccguuacccu ucccagccau uccacacgga agcugcagac ccgcucucag      420

acauggcucg agagcagaga auacaccaag caccugaucc ggguggagaa cuggaucuuu      480

cgcaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauuaga      600

ugcaucggag uuagcaaccg ggauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aagauaagcc uacaguggac      720

auugagcuug ugaccaccac agucuccaau auggccgaag ugcgcagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc agauguccca cgcagggaga agccuaccug      840

gacaaacagu cugauaccca guauguuugc aagcggacac ucguggacag aggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc      960

aagaaaauga caggaaagag cauccaaccu gagaaucucg aauaccgcau uaugcugucu     1020

guccacggcu cccagcacag cgggaugauc guuaacgaua ccggacauga gacggacgag     1080

aaucgggcca aaguggaaau uacaccgaac ucucccagag cugaggccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cgcaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagcgg cagacggucg ugguucucgg cucucaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

agacugagcu ccgggcaccu caaaugccgc cugaagaugg acaaacuucg gcugaaggga     1500

gucagcuauu cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaauac gccgggacag acggaccuug caaggucccc     1620

gcucagaugg ccguggauau gcagacccug acaccugugg gcagacucau caccgcgaac     1680

cccguuauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgc     1800

uccggcagca caaucgggaa agccuuugaa gcuaccgugc ggggagccaa gagaauggcu     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcccucaa cucucugggg     1920

aaaggcauuc accaaaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaga uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg     2040

agcaucuccc ugaugugucu ugcccuggga ggcguucuca uuuuccugag caccgcugug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2336
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long CDS opt

<400>  2336
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaagc gagaucucag      420

accuggcucg agagccggga auacacaaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcccu ggcugccgcu gccauugcgu ggcuccuggg guccagcacc      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcuua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac gugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggccc aggauaagcc uaccguggac      720

auugagcuug ugacaaccac cgucuccaau auggcugaag ugagaagcua cugcuaugag      780

gccucuaucu ccgauauggc cagcgacucc cgguguccca cacaaggaga ggcuuaccug      840

gacaaacagu cugauacgca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggucacau gcgcgaaguu ugccugcucc      960

aagaaaauga ccggaaagag cauccagccu gaaaaucucg aauaccggau uaugcugucu     1020

gugcacggcu cccaacacag cgggaugauc guuaacgaua caggacauga gaccgacgag     1080

aauagagcua aaguggaaau uacgccgaac ucuccccgcg ccgaggccac acuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu cggaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacca ugaauaacaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgcuggggc cgauacagga acaccccauu ggaauaacaa ggaagcgcug     1320

guggaguuua aagacgcuca cgccaagaga cagaccgucg ugguucucgg cucucaggag     1380

ggggccgugc auacggcucu ggccggcgcu cuugaagccg agauggaugg agcgaagggc     1440

cgccugagcu ccgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucagcuauu cucucuguac cgccgcuuuc acauuuacca aaauuccggc cgaaacacug     1560

cauggcaccg ugacgguuga ggugcaguac gcugggaccg acggaccuug caaggucccc     1620

gcccaaaugg cgguggauau gcagacacug accccugugg gccgccucau cacagccaac     1680

cccguuauca ccgaauccac ggagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacucuu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa ggcuuuugaa gccacaguga gaggagcuaa acgcauggcc     1860

guccugggcg acaccgccug ggauuucggg uccgugggag gcgcucucaa cucucugggg     1920

aaaggcauuc accagaucuu cggagcggcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuauugg aacccuccug auguggcugg gccucaauac aaagaacggg     2040

agcaucuccc ugaugugucu ugcucuggga ggcguucuca uuuuccugag cacggccgug     2100

ucugccgacg ucgggugcuc cguggauuuu agcaaa                               2136


<210>  2337
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long CDS opt

<400>  2337
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagauc ugacgccggg gaggcuauua gcuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugucc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucua ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

agcagacggg ccguuacccu ucccucccau ucuacacgca agcugcaaac cagaagccag      420

acauggcucg agucccggga auacaccaag caccugaucc gcguggagaa uuggaucuuu      480

agaaacccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg gagcucuacg      540

ucccagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cagcauucgg      600

ugcaucggag uuucuaaucg cgauuuugug gaaggcaugu ccgggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc guuauggcuc aggauaagcc uacaguggac      720

auugagcuug ugaccaccac agucagcaac auggccgaag ugagaucuua cugcuaugag      780

gcuuccauca gcgauauggc cuccgacucu cgguguccca cgcaaggaga agccuaccug      840

gacaaacaga gcgauaccca guauguuugc aagagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucc cuggugaccu gcgcuaaguu ugcgugcagc      960

aagaaaauga caggaaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

guccacggca gccaacacuc ugggaugauc guuaaugaua ccggacauga gacggacgag     1080

aacagagcca aaguggaaau uacaccgaau agcccccgcg cugaggccac ccuuggcggc     1140

uucggguccc ugggacucga uugugaaccu cggaccggcc uggacuuuuc ugaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc ugauaccgga acaccccauu ggaacaauaa ggaagcccug     1320

guggaguuua aagacgcgca cgcuaagaga cagacggucg ugguucucgg cagccaggag     1380

ggggccgugc auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugucca gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucucuuauu cccucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug     1560

cauggcacgg ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acaccugugg gccgccucau caccgcgaac     1680

cccguuauca cggaaagcac agagaacucu aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacuccu acauugucau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

agcggcucca caaucgggaa agccuuugaa gcuaccguga gaggagccaa gcgcauggcu     1860

guccugggcg acaccgccug ggauuucggg ucugugggag gcgcccucaa uagccugggg     1920

aaaggcauuc accagaucuu cggagcugcg uuuaaguccc uguucggcgg gaugucuugg     1980

uuuagccaaa uccuuauugg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

uccaucagcc ugaugugucu ugcccuggga ggcguucuca uuuuccuguc uaccgcugug     2100

uccgccgacg ucgggugcag cguggauuuu ucuaaa                               2136


<210>  2338
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long CDS opt

<400>  2338
agaaucauua acgcccggaa ggagaaaaag cgcagaggca ccgacacaag cguggggauc       60

gucggacugc uccugacgac cgcuauggcc gcggaaguua cacggcgcgg cuccgcuuac      120

uauauguacc uugauagauc ugacgccggg gaggccauua gcuuccccac cacacuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uaccaugucc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

acaaccucua cgugggucgu guauggaacc ugucaucaca agaaaggcga ggcccggcgc      360

agcagacggg cuguuacacu ucccucccau ucuacccgca agcugcaaac aagaagccag      420

accuggcucg agucccggga auacacgaag caccugaucc gcguggagaa uuggaucuuu      480

agaaacccug gcuucgcccu ggcggccgcu gccauugcuu ggcuccuggg gagcucuaca      540

ucccagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cagcauucgg      600

ugcaucggag uuucuaaucg cgauuuugug gaaggcaugu ccgggggaac cugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggccc aggauaagcc uaccguggac      720

auugagcuug ugaccacaac ggucagcaac auggcugaag ugagaucuua cugcuaugag      780

gccuccauca gcgauauggc uuccgacucu cgguguccca cccaaggaga agccuaccug      840

gacaaacaga gcgauacaca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucc cuggucacau gcgccaaguu ugcuugcagc      960

aagaaaauga ccggaaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

gugcacggca gccaacacuc ugggaugauc guuaaugaua cgggacauga gacagacgag     1080

aacagagcga aaguggaaau uaccccgaau agcccccgcg ccgaggcuac ccuuggcggc     1140

uucggguccc ugggacucga uugugaaccu cggacaggcc uggacuuuuc ugaucuuuau     1200

uaccugacca ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acacaggggc cgauacggga acaccccauu ggaacaauaa ggaagcccug     1320

guggaguuua aagacgcuca cgccaagaga cagaccgucg ugguucucgg cagccaggag     1380

ggggcggugc auaccgcucu ggccggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugucca gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucucuuauu cccucuguac agcugccuuc accuuuacga aaauuccggc ggaaacccug     1560

cauggcacag ugaccguuga ggugcaguac gccgggacag acggaccuug caaggucccc     1620

gcucaaaugg ccguggauau gcagacccug acgccugugg gccgccucau cacagcuaac     1680

cccguuauca ccgaagggac agagaacagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggcgacucuu acauugucau cggagugggc gagaaaaaga uuacccacca cuggcaucgg     1800

uccgggagca ccaucggaaa agccuuugaa gcgacaguga gaggcgccaa gcgcauggcu     1860

guccuggggg acacggccug ggauuucgga uccgugggcg ggguucucaa uucucugggc     1920

aaaggcauuc accagaucuu cggagcugcc uuuaagagcc uguucggggg aauguccugg     1980

uuuucucaaa uccuuauugg cacccuccug auguggcugg ggcucaacac aaagaaugga     2040

agcaucuccc ugaugugucu ugcccugggc ggggugcuca uuuuccugag caccgcuguc     2100

ucugcggacg ugggcugcuc cguugauuuu agcaaa                               2136


<210>  2339
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long CDS opt

<400>  2339
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg gcugaaguua cccggcgcgg cuccgccuac      120

uauauguacc uugauagaaa ugacgccggg gaggcuauuu cuuuccccac aacccuggga      180

augaacaaau gcuauaucca gauuauggau cucggccaca ugugugacgc cacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccguuacccu ucccagccau uccacacgca agcugcaaac cagaucucag      420

acauggcucg agagccggga auacaccaag caccugaucc gcguggagaa cuggaucuuu      480

agaaauccug gcuucgcgcu ggccgcugcc gcuauugccu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cgcccgccua cuccauucgg      600

ugcaucggag uuagcaaccg cgauuuugug gaaggcaugu cugggggaac augggucgac      660

guggugcugg agcauggcgg gugugucacc gcuauggccc aggauaagcc uacaguugac      720

auugagcuug ugaccaccac aguguccaau auggcugaag ucagaagcua cugcuaugag      780

gccucuaucu ccgauauggc cagcgacucc cgguguccca cgcaaggaga ggcuuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg cggcuggggc      900

aacgggugug gacuguucgg caaagggagc cuggugaccu gcgcgaaguu ugccugcucc      960

aagaaaauga caggaaagag cauccagccu gaaaaucucg aauaccggau uaugcugucu     1020

guccacggcu cccaacacag cgggaugcuu gugaacgaua ccggacauga gacggacgag     1080

aauagagcua aaguugaaau cacaccgaac ucuccccgcg ccgaggccac ccugggcggc     1140

uucgggagcc ucggacugga uugugaaccu cggaccggcc uugacuuuuc cgaucuguau     1200

uaccucacaa ugaauaacaa gcacuggcug gcucauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc ggcuaccgga acaccccauu ggaauaacaa ggaagcccuc     1320

guggaguuua aagaugccca cgcuaagaga cagacggucg ugguccuggg cucucaggag     1380

ggggccgugc auaccgcucu ugccggcgcg cuggaagccg agauggacgg agcuaagggc     1440

cgccucagcu ccgggcaccu gaaaugccgg cuuaagaugg auaaacugag acucaaggga     1500

guuagcuauu cucuguguac agccgcuuuc accuuuacaa aaaucccggc cgaaaccgug     1560

gacggcacgg ucaccgugga ggggcaguac ggaggcacag acgggccuug caagguuccc     1620

gcgcaaaugg ccguggauau gcagacccug acaccugucg gccgccucau uaccgcuaac     1680

cccgugauca cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc     1740

ggagacucuu acaucgugau uggcguuggg gagaaaaaga ucacccauca cuggcaccgg     1800

uccggaagca caauuggcaa agccuuugaa gcuaccguca gaggggccaa gcgcauggcc     1860

gugcugggag acaccgcuug ggauuucggc uccgucgggg gcgcgcucaa cucucuggga     1920

aaaggcaucc aucagauuau cggggccgcu uucaagagcc uguuuggagg cauguccugg     1980

uucucucaaa uccuuauugg gacacuccug auguggcugg gacucaauac gaagaacggc     2040

agcaucuccc ugaugugucu ugcccucggg ggcgugcuga uuuuucugag caccgccgug     2100

ucuggaggcg uugggugcuc cguggacuuc agcaaa                               2136


<210>  2340
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long CDS opt

<400>  2340
agaaucauua acgcccggaa ggagaaaaag cgcagaggca ccgacacaag cguggggauc       60

gucggacugc uccugacgac cgcuauggcc gcggaaguua cacggcgcgg cuccgcuuac      120

uauauguacc uugauagauc ugacgccggg gaggccauua gcuuccccac cacacuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uaccaugucc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

acaaccucua cgugggucgu guauggaacc ugucaucaca agaaaggcga ggcccggcgc      360

agcagacggg cuguuacacu ucccucccau ucuacccgca agcugcaaac aagaagccag      420

accuggcucg agucccggga auacacgaag caccugaucc gcguggagaa uuggaucuuu      480

agaaacccug gcuucgcccu ggcggccgcu gccauugcuu ggcuccuggg gagcucuaca      540

ucccagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cagcauucgg      600

ugcaucggag uuucuaaucg cgauuuugug gaaggcaugu ccgggggaac cugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggccc aggauaagcc uaccguggac      720

auugagcuug ugaccacaac ggucagcaac auggcugaag ugagaucuua cugcuaugag      780

gccuccauca gcgauauggc uuccgacucu cgguguccca cccaaggaga agccuaccug      840

gacaaacaga gcgauacaca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucc cuggucacau gcgccaaguu ugcuugcagc      960

aagaaaauga ccggaaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

gugcacggca gccaacacuc ugggaugauc guuaaugaua cgggacauga gacagacgag     1080

aacagagcga aaguggaaau uaccccgaau agcccccgcg ccgaggcuac ccuuggcggc     1140

uucggguccc ugggacucga uugugaaccu cggacaggcc uggacuuuuc ugaucuuuau     1200

uaccugacca ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc cgauacagga acgccccauu ggaacaauaa ggaagcucug     1320

guggaguuua aagacgccca cgcgaagaga cagaccgucg ugguucucgg cagccaggag     1380

ggggcugugc auacagcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cgccugucca gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucucuuauu cccucuguac cgccgcguuc acauuuagca aaauuccggc cgaaacccug     1560

cauggcacgg ugaccgagga aguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccgccucau caccgccaac     1680

cccgugauca cggagucuac agagaacucc aagaugaugc uggaacuuga ucccccuuuc     1740

ggggacagcu acauuguuau cggcgucgga gagaaaaaga uuacccacca cuggcaucgg     1800

uccggcucua caaucgggaa agcguuugaa gccaccguga gaggagcuaa gcgcauggcc     1860

gugcugggcg acaccgcuug ggauuucggg agcgucggag gcgcccucaa uucccugggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaagucuc uguucggcgg gaugagcugg     1980

uuuucccaaa uccuuauugg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcaucucuc ugaugugucu ugcgcuggga ggcgugcuca uuuuccuguc caccgccguu     2100

agcgcugacg uggggugcuc ugucgauuuu uccaaa                               2136


<210>  2341
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long CDS opt

<400>  2341
agaaucauua acgcccggaa ggagaaaaag cgcagaggca ccgacacaag cguggggauc       60

gucggacugc uccugacgac cgcuauggcc gcggaaguua cacggcgcgg cuccgcuuac      120

uauauguacc uugauagauc ugacgccggg gaggccauua gcuuccccac cacacuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uaccaugucc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

acaaccucua cgugggucgu guauggaacc ugucaucaca agaaaggcga ggcccggcgc      360

agcagacggg cuguuacacu ucccucccau ucuacccgca agcugcaaac aagaagccag      420

accuggcucg agucccggga auacacgaag caccugaucc gcguggagaa uuggaucuuu      480

agaaacccug gcuucgcccu ggcggccgcu gccauugcuu ggcuccuggg gagcucuaca      540

ucccagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cagcauucgg      600

ugcaucggag uuucuaaucg cgauuuugug gaaggcaugu ccgggggaac cugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggccc aggauaagcc uaccguggac      720

auugagcuug ugaccacaac ggucagcaac auggcugaag ugagaucuua cugcuaugag      780

gccuccauca gcgauauggc uuccgacucu cgguguccca cccaaggaga agccuaccug      840

gacaaacaga gcgauacaca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucc cuggucacau gcgccaaguu ugcuugcagc      960

aagaaaauga ccggaaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

gugcacggca gccaacacuc ugggaugauc guuaaugaua cgggacauga gacagacgag     1080

aacagagcga aaguggaaau uaccccgaau agcccccgcg ccgaggcuac ccuuggcggc     1140

uucggguccc ugggacucga uugugaaccu cggacaggcc uggacuuuuc ugaucuuuau     1200

uaccugacca ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc cgauacagga acgccccauu ggaacaauaa ggaagcucug     1320

guggaguuua aagacgccca cgcgaagaga cagaccgucg ugguucucgg cagccaggag     1380

ggggcugugc auacagcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cgccugucca gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucucuuauu cccucuguac cgccgcguuc acauuuacca aaauuccggc cgaaacgcug     1560

cauggcaccg ugacaguuga ggugcaguac gcugggaccg acggaccuug caaggucccc     1620

gcccaaaugg cuguggauau gcagacacug accccugugg gccgccucau cacggccaac     1680

cccguuauca cagaaagcac cgagaacucu aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacuccu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

agcggcucca ccaucgggaa agcguuugaa gccaccguga gaggagcuaa gcgcauggcc     1860

guccugggcg acacagcuug ggauuucggg ucugugggag gcgcccucaa uagccugggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuagccaaa uccuuauugg aacgcuccug auguggcugg gccucaacac caagaauggg     2040

uccaucagcc ugaugugucu ugcgcuggga ggcguucuca uuuuccuguc uacagccgug     2100

uccgcugacg ucgggugcag cguggauuuu ucuaaa                               2136


<210>  2342
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long CDS opt

<400>  2342
agaaucauua acgcccggaa ggagaaaaag cgcagaggca ccgacacaag cguggggauc       60

gucggacugc uccugacgac cgcuauggcc guugaaguga cacggcgcgg caaugcguac      120

uauauguacc uugauagauc cgacgcuggg gaggccauuu cuuuccccac cacaauggga      180

augaacaaau gcuauaucca gauuauggau cugggccaca ugugugacgc caccaugagc      240

uacgaaugcc cuaugcucga ugagggggug gaaccggacg augucgacug uuggugcaau      300

acaaccucca cguggguggu uuauggaacc ugucaucaca agaaaggcga ggcucggcgc      360

ucuagacggg ccgugacacu gcccagccau uccacccgca aacuucaaac aagaucucag      420

accuggcugg agagccggga auacacgaag caccucaucc gcgucgagaa cuggaucuuu      480

agaaauccug gcuucgcucu ggccgcggcc gcuauugccu ggcugcucgg guccagcaca      540

ucucagaaag ugaucuaucu gguuaugauu cuucugaucg cucccgccua cuccauucgg      600

ugcaucggag ugagcaaccg cgauuuuguc gaaggcaugu cugggggaac cuggguggac      660

gugguccucg agcauggcgg guguguuaca gugauggcgc aggauaagcc uaccguggac      720

auugagcugg ucaccacaac gguguccaau auggccgaag uuagaagcua cugcuaugag      780

gcuucuaucu ccgauauggc cagcgacucc cgguguccca cccaaggaga ggcuuaccuu      840

gacaaacagu cugauacaca guaugugugc aagagaaccc uggucgaccg cggcuggggc      900

aacgggugug gacucuucgg caaagggagc cuggugacau gcgccaaguu ugccugcucc      960

aagaaaauga ccggaaagag cauccagccu gaaaaucugg aauaccggau uaugcucucu     1020

guucacggcu cccaacacag cgggaugauc gugaacgaua cgggacauga gacagacgag     1080

aauagagcua aagucgaaau uaccccgaac ucuccccgcg cggaggccac ccugggcggc     1140

uucgggagcc uuggacugga uugugaaccu cggacaggcc ucgacuuuuc cgaucuguau     1200

uaccuuacca ugaauaacaa gcacuggcug gugcauaaag agugguucca cgacaucccc     1260

cucccuuggc acgcuggggc cgauacagga acgccccauu ggaauaacaa ggaagcccug     1320

gucgaguuua aagacgcuca cgccaagaga cagaccgugg uugugcuggg cucucaggag     1380

ggggcggucc auacagcucu cgccggcgcc cuggaagcug agauggaugg agccaagggc     1440

cgccuuagcu ccgggcaccu gaaaugccgg cucaagaugg acaaacugag acuuaaggga     1500

gugagcuauu cucuguguac cgcugccuuc acauuuacca aaauuccggc ggaaacgcuc     1560

cauggcaccg uuacagugga gguccaguac gccgggaccg acggaccuug caaggugccc     1620

gcucaaaugg ccguggauau gcagacacug accccuguug gccgccugau cacggcuaac     1680

cccgucauca cagaauccac cgagaauagc aagaugaugc ucgagcugga ucccccuuuc     1740

ggggacucuu acauugugau cggcguggga gaaaagaaga uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa agccuuugag gcgaccguca gaggagccaa gcgcauggcu     1860

gugcuuggcg acacagccug ggauuucggg uccguuggag gcgcucugaa cucucucggg     1920

aaaggcauuc accagaucuu cggagccgcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccugauugg aacgcuucuc guguggcugg gccugaauac caagaacggg     2040

agcaucuccc ucaugugucu ggcucuugga ggcguccuga uuuuccucag cacagcggug     2100

ucugccgacg uugggugcuc cguggauuuu agcaaa                               2136


<210>  2343
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long CDS opt

<400>  2343
agaaucauua acgcccggaa ggagaaaaag cgcagaggca ccgacacaag cguggggauc       60

gucggacugc uccugacgac cgcuauggcc gcggaaguua cacggcgcgg cuccgcuuac      120

uauauguacc uugauagauc ugacgccggg gaggccauua gcuuccccac cacacuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uaccaugucc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

acaaccucua cgugggucgu guauggaacc ugucaucaca agaaaggcga ggcccggcgc      360

agcagacggg cuguuacacu ucccucccau ucuacccgca agcugcaaac aagaagccag      420

accuggcucg agucccggga auacacgaag caccugaucc gcguggagaa uuggaucuuu      480

agaaacccug gcuucgcccu ggcggccgcu gccauugcuu ggcuccuggg gagcucuaca      540

ucccagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cagcauucgg      600

ugcaucggag uuucuaaucg cgauuuugug gaaggcaugu ccgggggaac cugggucgac      660

guggugcugg agcauggcgg gugugucaca guuauggccc aggauaagcc uaccguggac      720

auugagcuug ugaccacaac ggucagcaac auggcugaag ugagaucuua cugcuaugag      780

gccuccauca gcgauauggc uuccgacucu cgguguccca cccaaggaga agccuaccug      840

gacaaacaga gcgauacaca guauguuugc aagagaaccc ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucc cuggucacau gcgccaaguu ugcuugcagc      960

aagaaaauga ccggaaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

gugcacggca gccaacacuc ugggaugauc guuaaugaua cgggacauga gacagacgag     1080

aacagagcga aaguggaaau uaccccgaau agcccccgcg ccgaggcuac ccuuggcggc     1140

uucggguccc ugggacucga uugugaaccu cggacaggcc uggacuuuuc ugaucuuuau     1200

uaccugacca ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc cgauacagga acgccccauu ggaacaauaa ggaagcucug     1320

guggaguuua aagacgccca cgcgaagaga cagaccgucg ugguucucgg cagccaggag     1380

ggggcugugc auacagcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cgccugucca gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gucucuuauu cccucuguac cgccgcguuc acauuuacca aaauuccggc cgaaacgcug     1560

cauggcaccg ugacaguuga ggugcaguac gcugggaccg acggaccuug caaggucccc     1620

gcccaaaugg cuguggauau gcagacacug accccugugg gccgccucau cacggccaac     1680

cccguuauca cagaaagcac cgagaacucu aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacuccu acauugucau cggcguggga gagaaaaaga uuacacacca cuggcaucgg     1800

agcggcucca ccaucgggaa agcguuugaa gccaccguga gaggagcuaa gcgcauggcc     1860

guccugggcg acacagcuug ggauuucggg ucugugggag gcgcccucaa uagccugggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuagccaaa uccuuauugg aacgcuccug auguggcugg gccucaacac caagaauggg     2040

uccaucagcc ugguuugucu ugcgcuggga ggcgugcuca uuuuccuguc uacagccguc     2100

uccgcugacg uggggugcag cguugauuuu ucuaaa                               2136


<210>  2344
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long CDS opt

<400>  2344
agaaucauua acgcccggaa ggagaaaaag cgcagaggca ccgacacaag cguggggauc       60

gucggacugc uccugacgac cgcuauggcc guugaaguga cacggcgcgg cuccgcguac      120

uauauguacc uugauagauc ugacgcuggg gaggccauua gcuuccccac cacacuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc caccaugucc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg augucgacug uuggugcaac      300

acaaccucua cguggguggu uuauggaacc ugucaucaca agaaaggcga ggcucggcgc      360

agcagacggg ccgugacacu ucccucccau ucuacccgca agcugcaaac aagaagccag      420

accuggcucg agucccggga auacacgaag caccugaucc gcgucgagaa uuggaucuuu      480

agaaacccug gcuucgcucu ggccgcggcc gcuauugccu ggcuccuggg gagcucuaca      540

ucccagaaag ugaucuaucu uguuaugauu cugcucaucg cucccgccua cagcauucgg      600

ugcaucggag ugucuaaucg cgauuuuguc gaaggcaugu ccgggggaac cuggguggac      660

gugguccugg agcauggcgg guguguuaca gugauggcgc aggauaagcc ugccguggac      720

auugagcuug ucaccaccac agugagcaac auggcugaag uuagaucuua cugcuaugag      780

gccuccauca gcgauauggc uuccgacucu cgguguccca cgcaaggaga agccuaccug      840

gacaaacaga gcgauaccca guaugugugc aagagaacac ucgucgaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucc cuggugaccu gcgccaaguu ugcuugcagc      960

aagaaaauga caggaaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

guucacggca gccaacacuc ugggaugauc gugaaugaua ccggacauga gacggacgag     1080

aacagagcga aagucgaaau uacaccgaau agcccccgcg ccgaggcuac ccuuggcggc     1140

uucggguccc ugggacucga uugugaaccu cggaccggcc uggacuuuuc ugaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagaga cagacggugg uugugcucgg cagccaggag     1380

ggggcugucc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cgccugucca gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

gugucuuauu cccucuguac agccgcguuc accuuuacaa aaauuccggc cgaaacccug     1560

cauggcacgg uuaccgugga gguccaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cuguggauau gcagacccug acaccuguug gccgccucau caccgccaac     1680

cccgucauca cggaaagcac agagaacucu aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacuccu acauugugau cggcguggga gagaaaaaga uuacccacca cuggcaucgg     1800

agcggcucca caaucgggaa agcguuugaa gccaccguca gaggagcuaa gcgcauggcc     1860

gugcugggcg acaccgcuug ggauuucggg ucuguuggag gcgcccucaa uagccugggg     1920

aaaggcauuc accagaucuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuagccaaa uccuuauugg aacacuccug guguggcugg gccucaacac gaagaauggg     2040

uccaucagcc ugaccugucu ugcgcuggga ggcguccuca uuuuccuguc uacagccgug     2100

uccgcugacg uugggugcag cguggauuuu ucuaaa                               2136


<210>  2345
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long CDS opt

<400>  2345
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg guugaaguga cccggcgcgg cuccgcuuac      120

uauauguacc uugauagauc ugacgccggg gaggccauua gcuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugucc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg augucgacug uuggugcaac      300

accacaucua ccuggguggu uuauggaacg ugucaucaca agaaaggcga ggcccggcgc      360

agcagacggg cugugacccu ucccucccau ucuacacgca agcugcaaac cagaagccag      420

acauggcucg agucccggga auacaccaag caccugaucc gcgucgagaa uuggaucuuu      480

agaaacccug gcuucgcccu ggcggccgcu gugauugccu ggcuccuggg gagcucuacg      540

ucccagaaag uuaucuaucu ugugaugauu cugcucaucg cucccgccua cagcauucgg      600

ugcaucggag ucucuaaucg cgauuuugug gaaggcaugu ccgggggaac auggguggac      660

gucguucugg agcauggcgg gugugugacc gugauggcgc aggauaagcc uacagucgac      720

auugagcuug ugaccaccac aguuagcaac auggccgaag ugagaucuua cugcuaugag      780

gcuuccauca gcgauauggc cuccgacucu cgguguccca cgcaaggaga ggcuuaccug      840

gacaaacaga gcgauaccca guaugucugc aagagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucc cugguuaccu gcgccaaguu ugccugcagc      960

aagaaaauga caggaaaguc uauccagccu gaaaaccucg aauaccggau uaugcugucc     1020

gugcacggca gccaacacuc ugggaugauc gucaaugaua ccggacauga gacggacgag     1080

aacagagcua aaguggaaau uacaccgaau agcccccgcg cggaggccac ccuuggcggc     1140

uucggguccc ugggacucga uugugaaccu cggaccggcc uggacuuuuc ugaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgcuggggc cgauaccgga acaccccauu ggaacaauaa ggaagcccug     1320

guggaguuua aagacgcuca cgccaagaga cagacgguug ugguccucgg cagccaggag     1380

ggggcggugc auaccgcucu ggccggcgcc cuugaagcug agauggaugg agccaagggc     1440

cgccugucca gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

guuucuuauu cccucuguac agcugccuuc accuuuacaa aaauuccggc ggaaacccug     1560

cauggcacgg ugaccgucga ggugcaguac gccgggacag acggaccuug caaggugccc     1620

gcucaaaugg ccguugauau gcagacccug acaccugucg gccgccucau caccgcuaac     1680

cccgugauca cggaaagcac agagaacucu aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacuccu acauugugau cggcgucgga gagaaaaaga uuacccacca cuggcaucgg     1800

agcggcucca caaucgggaa agccuuugaa gcgaccguga gaggagccaa gcgcauggcu     1860

guucugggcg acaccgccug ggauuucggg ucugugggag gcgcucucaa uagccugggg     1920

aaaggcauuc accagaucuu cggagccgcc uuuaaguccc uguucggcgg gaugucuugg     1980

uuuagccaaa uccuuauugg aacacuccug gucuggcugg gccucaacac gaagaauggg     2040

uccaucagcc ugaccugucu ugcucuggga ggcgugcuca uuuuccuguc uacagcgguu     2100

uccgccgacg uggggugcag cgucgauuuu ucuaaa                               2136


<210>  2346
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long CDS opt

<400>  2346
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg guugaaguga cccggcgcgg cuccgcuuac      120

uauauguacc uugauagauc ugacgccggg gaggccauua gcuuccccac aacccuggga      180

gugaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugucc      240

uacgaaugcc cuaugcugga ugaggggguc gaaccggacg auguggacug uuggugcaac      300

accacaucua ccuggguugu guauggaacg ugucaucaca agaaaggcga ggcccggcgc      360

agcagacggg cugucacccu ucccucccau ucuacacgca agcugcaaac cagaagccag      420

acauggcucg agucccggga auacaccaag caccugaucc gcguggagaa uuggaucuuu      480

agaaacccug gcuucgcccu ggcggccgcu gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaaag uuaucuaucu ugugaugauu cugcucaucg cccccgcgua cagcauucgg      600

ugcaucggag ucucuaaucg cgauuuugug gaaggcaugu ccgggggaac auggguggac      660

gucguucugg agcauggcgg gugugugacc gugauggccc aggauaagcc uacagucgac      720

auugagcuug ugaccaccac aguuagcaac auggcugaag ugagaucuua cugcuaugag      780

gccuccauca gcgauauggc uuccgacucu cgguguccca cgcaaggaga agccuaccug      840

gacaaacaga gcgauaccca guaugucugc aagagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaagggucc cugguuaccu gcgccaaguu ugcuugcagc      960

aagaaaauga caggaaaguc uauccagccu gagaaccucg aauaccggau uaugcugucc     1020

gugcacggca gccaacacuc ugggaugauc gucaaugaua ccggacauga gacggacgag     1080

aacagagcga aaguggaaau uacaccgaau agcccccgcg ccgaggcuac ccuuggcggc     1140

uucggguccc ugggacucga uugugaaccu cggaccggcc uggacuuuuc ugaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc guccauaaag agugguucca cgacaucccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

guggaguuua aagacgccca cgcgaagaga cagacgguug ugguccucgg cagccaggag     1380

ggggcugugc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cgccugucca gcgggcaccu caaaugccgg cugaagaugg acaaacuuag acugaaggga     1500

guuucuuauu cccucuguac agccgcguuc accuuuacaa aaauuccggc cgaaacccug     1560

cauggcacgg ugaccgucga ggugcaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cuguugauau gcagacccug acaccugucg gccgccucau caccgccaac     1680

cccgugauca cggaaagcac agagaacucu aagaugaugc uggagcuuga ucccccuuuc     1740

ggggacuccu acauugugau cggcgucgga gacaaaaaga uuacccacca cugguaucgg     1800

agcggcucca caaucgggaa agcguuugaa gccaccguga gaggagcuaa gcgcauggcc     1860

guucugggcg auaccgcuug ggacuucggg ucugugggag gcgcccucaa uagccugggg     1920

aaaggcauuc aucagaucuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuagccaaa uccuuauugg aacacuccug gucuggcugg gccucaacac gaagaauggg     2040

uccaucagcc ugaccugucu ugcgcuggga ggcgugcuca uuuuccuguc uacagccguu     2100

uccgcugaug uggggugcag cgucgacuuu ucuaaa                               2136


<210>  2347
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long CDS opt

<400>  2347
agaaucauua acgcccggaa ggagaaaaag cgcagaggcg cugacaccag cguggggauc       60

gucggacugc uccugacaac ggccauggcg guugaaguga cccggcgcgg cuccgcuuac      120

uauauguacc uugauagauc ugacgccggg aaagccauua gcuuccccac aacccuggga      180

gugaauaagu gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugucc      240

uacgagugcc cuaugcugga ugaagggguc gagccggacg auguggacug uuggugcaac      300

accacaucua ccuggguugu guauggaacg ugucaucaca aaaagggcga agcccggcgc      360

agcagacggg cugucacccu ucccucccau ucuacacgca agcugcaaac cagaagccag      420

acauggcucg agucccggga guacaccaaa caccugaucc gcguggaaaa uuggaucuuu      480

agaaacccug gcuucgcccu ggcggccgcu gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaagg uuaucuaucu ugugaugauu cugcucaucg cccccgcgua cagcauucgg      600

ugcaucggag ucucuaaucg cgauuuugug gagggcaugu ccgggggaac auggguggac      660

gucguucugg aacauggcgg gugugugacc gugauggccc aggauaaacc uacagucgac      720

auugagcuug ugaccaccac aguuagcaac auggcugagg ugagaucuua cugcuaugaa      780

gccuccauca gcgauauggc uuccgacucu cgguguccca cgcaaggaga ggccuaccug      840

gacaagcaga gcgauaccca guaugucugc aaaagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cugguuaccu gcgccaaguu ugcuugcagc      960

aaaaagauga caggaaaauc uauccagccu gaaaaccucg aguaccggau uaugcugucc     1020

gugcacggca gccaacacuc ugggaugauc gucaaugaua uuggacauga aaccgacgag     1080

aacagagcga agguggagau cacgccgaau agcccccgcg ccgaagcuac acuuggcggc     1140

uucggguccc ugggacucga uugugagccu cggaccggcc uggacuuuuc ugaucuuuau     1200

uaccugacca ugaacaauaa acacuggcuc guccauaagg aaugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauacagga acaccccauu ggaacaauaa agaggcucug     1320

guggaauuua aggacgccca cgcgaagaga cagacaguug ugguccucgg cagccaggag     1380

ggggcugugc auacggcccu ggccggcgcu cuugaggccg aaauggaugg agcuaaaggc     1440

cgccugucca gcgggcaccu caagugccgg cugaaaaugg acaaacuuag acugaaggga     1500

guuucuuauu cccucuguac cgccgcguuc acauuuacca agaucccggc cgagacacug     1560

cauggcaccg ugacggucga agugcaguac gcugggaccg acggaccuug caaggugccc     1620

gcccaaaugg cuguugauau gcagacacug accccugucg gccgccucau cacagccaac     1680

cccgugauua ccgagagcac ggaaaacucu aaaaugaugc uggagcuuga ucccccuuuc     1740

ggggacuccu acaucgugau uggcgucgga gacaagaaaa ucacacacca cuggcaucgg     1800

agcggcucca ccauugggaa ggcguuugag gccacaguga gaggagcuaa acgcauggcc     1860

guucugggcg auaccgcuug ggacuucggg ucugugggag gcgcccucaa uagccugggg     1920

aagggcaucc accagaucuu cggagccgcu uuuaaguccc uguucggcgg gaugucuugg     1980

uuuagccaaa uucuuaucgg aacccuccug gucuggcugg gccucaacac caaaaauggg     2040

uccauuagcc ugacgugucu ugcgcuggga ggcgugcuca ucuuccuguc uaccgccguu     2100

uccgcugaug uggggugcag cgucgacuuu ucuaag                               2136


<210>  2348
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long CDS opt

<400>  2348
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

gugggacugc uccugacaac ggccauggcg gcugaaauca cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauua gcuucgccac aacccuggga      180

gucaauaaau gccacguuca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaaggggug gagcccgacg auguggacug uuggugcaac      300

accacaucua ccugggucgu guacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cgguuacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauca aaguggaaaa uuggauuuuu      480

agaaaccccg gcuucacgcu ggucgccgug gcuaucgccu ggcuccuggg gagcucuaca      540

ucccagaagg uuauuuaccu ugugaugauc cugcucauug cuccugccua uagcauccgg      600

ugcauuggag ucucuaaucg cgauuuugug gagggcaugu ccgggggaac cuggguggac      660

gucguucugg aacacggcgg gugugugaca gugauggcgc aggauaaacc caccgucgac      720

aucgagcuug ugaccacaac gguuagcaac auggccgagg ugagaucuua cugcuacgaa      780

gccuccauca gcgauauggc uuccgacucu cgguguccua cccaaggaga ggcuuaucug      840

gacaagcaga gcgauacaca guacgucugc aaaagaaccc ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cugguuacau gcgccaaguu uaccugcagc      960

aaaaagauga cgggaaaauc uauucagccc gaaaaccucg aguaucggau caugcugucc     1020

gugcacggca gccaacauuc ugggaugauu gucaaugaua caggacacga aaccgacgag     1080

aacagagcca agguggaggu caccccuaau agcccgcgcg cugaagcgac acuuggcggc     1140

uucggguccc ugggacucga uugugagccc cggaccggcc uggacuuuuc ugaucuuuac     1200

uaucugacaa ugaacaauaa acauuggcuc gugcacaagg aaugguucca cgacaucccu     1260

cugcccuggc augccggggc ugauacggga accccucacu ggaacaauaa agaggcccug     1320

guugaauuua aggacgccca ugcuaagaga cagacagugg ucgugcucgg cagccaggag     1380

ggggccguuc acaccgcgcu ggcuggcgcc cuugaggccg aaauggaugg agcuaaaggc     1440

cgccuguucu ccgggcaucu caagugccgg cugaaaaugg acaaacuuag acugaaagga     1500

gugagcuacu cucucuguac agccgcuuuu accuucacga agguccccgc cgagacccug     1560

cacggcacag ugaccgugga aguucaguau gcggggacag acggaccgug caaggucccu     1620

gcccaaaugg cuguggauau gcagacccug acgcccgugg gccgccucau uacagccaac     1680

ccugucauca ccgaguccac agaaaacagc aaaaugaugc uggagcuuga cccucccuuu     1740

ggggauucuu acaucgugau uggcguugga gacaagaaaa ucacccacca uuggcaccgg     1800

uccggcagca ccauugggaa ggcuuucgag gccacaguga gaggagcgaa acgcauggcc     1860

guccugggcg auacggcuug ggacuucggg uccgugggag gcguuuuuaa uucucucggg     1920

aagggcaucc aucagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccugaucgg aacccuucuc guguggcugg gccugaacac aaagaauggg     2040

agcauuuccc ucaccugucu ggcccuugga ggcgucauga ucuuccugag cacagccgug     2100

ucugcugaug uugggugcuc cguggacuuu agcaag                               2136


<210>  2349
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long CDS opt

<400>  2349
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aaaucuggga      180

gugaacaaau gccacgucca gaucauggau cucggccaua ugugugacgc uaccaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaau      300

acaaccucua cauggguggu cuacggaacc ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cggugacgcu uccgucccac ucuaccagaa agcugcaaac acggagccag      420

accuggcucg agucccgcga guauacaaag caucugauua aaguugaaaa cuggaucuuu      480

agaaaucccg gcuucgcccu ggcugccgug gcuauugccu ggcuccuggg gagcucuacc      540

ucccagaagg ucaucuaccu ugugaugauu cugcucaucg cgccugccua uagcauucgg      600

ugcaucggag uuucuaaccg cgauuuugug gagggcaugu ccgggggaac gugggucgac      660

guggugcugg aacacggcgg gugugucaca guuauggcuc aggauaaacc caccguggac      720

aucgagcuug ugacaaccac cgucagcaau auggccgagg ugagaucuua cugcuacgag      780

gcuuccauua gcgauauggc cuccgacucu cgguguccua cacaaggaga agccuaucug      840

gacaagcaga gcgauacgca guacguuugc aaaagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaggggucc cuggucacau gcgcuaaguu uaccugcagc      960

aaaaagauga caggaaaauc uauccagccc gaaaaucucg aguaucggau uaugcugucc     1020

gugcacggca gccaacauuc ugggaugauc guuaacgaua uuggacacga aaccgacgag     1080

aauagagcga agguggaggu cacgccuaac agcccgcgcg ccgaagcuac acuuggcggc     1140

uucggguccc ugggacucga uugugagccc cggaccggcc uggacuuuuc ugaucuuuac     1200

uaucugacca ugaauaacaa acauuggcuc gugcacaagg aaugguucca cgacaucccu     1260

cugcccuggc augccggggc cgauacagga accccucacu ggaauaacaa agaggcucug     1320

gucgaauuua aggacgccca ugcgaagaga cagacagugg uugugcucgg cagccaggag     1380

ggggcugucc acacggcccu ggccggcgcu cuugaggccg aaauggaugg agcuaaaggc     1440

cgccuguucu ccgggcaucu caagugccgg cugaaaaugg acaaacuuag acugaaagga     1500

gugagcuacu cucucuguac cgccgcguuu acauucacca agguucccgc cgagacacug     1560

cacggcaccg ugacggucga agugcaguau gcugggaccg acggaccgug caaggugccu     1620

gcccaaaugg cuguugauau gcagacacug acccccgucg gccgccucau cacagccaac     1680

ccugugauua ccgaguccac ggaaaauagc aaaaugaugc uggagcuuga cccucccuuu     1740

ggggauucuu acaucgugau uggcgucgga gacaagaaaa ucacacacca uuggcaccgg     1800

uccggcagca ccauugggaa ggcguucgag gccacaguga gaggagcuaa acgcauggcc     1860

guucugggcg auaccgcuug ggacuucggg uccgugggag gcgucuuuaa cucucucggg     1920

aagggcgugc aucagaucuu cggagccgcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccugauugg aacccuucuc guuuggcugg gccugaauac aaagaacggg     2040

agcaucuccc ucacgugucu ggcucuugga ggcgugauga uuuuccugag caccgcgguc     2100

ucugccgaug uggggugcuc cguugacuuu agcaag                               2136


<210>  2350
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long CDS opt

<400>  2350
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aacccuggga      180

gugaauaaau gccacgucca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cggugacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauua aaguugaaaa uuggaucuuu      480

agaaaccccg gcuucgcccu gguggcuguc gccauugcuu ggcuccuggg gagcucuacg      540

ucccagaagg ugaucuaccu uguuaugauu cugcucaucg ccccugcgua uagcauucgg      600

ugcaucggag ugucuaaucg cgauuuuguc gagggcaugu ccgggggaac auggguggac      660

gugguccugg aacacggcgg guguguuacc gugauggccc aggauaaacc cacaguggac      720

aucgagcuug ucaccaccac agugagcaac auggcugagg uuagaucuua cugcuacgaa      780

gccuccauua gcgauauggc uuccgacucu cgguguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgugugc aaaagaacac ucgucgaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cuggugaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauccagccc gaaaaccucg aguaucggau uaugcugucc     1020

guucacggca gccaacauuc ugggaugauc gugaaugaua uuggacacga aacggacgag     1080

aacagagcua aggucgaggu gacaccuaau agcccgcgcg cggaagccac ccuuggcggc     1140

uucggguccc ugggacucga uugugagccc cggaccggcc uggacuuuuc ugaucuuuac     1200

uaucugacaa ugaacaauaa acauuggcuc guccacaagg aaugguucca cgacaucccu     1260

cugcccuggc augcuggggc cgauaccgga acaccucacu ggaacaauaa agaggcccug     1320

guggaauuua aggacgcuca ugccaagaga cagacgguug ugguccucgg cagccaggag     1380

ggggcggugc acaccgcucu ggccggcgcc cuugaggcug aaauggaugg agccaaaggc     1440

cgccuguucu ccgggcaucu caagugccgg cugaaaaugg acaaacuuag acugaaggga     1500

guuagcuacu cucucuguac agcugccuuu accuucacaa aggugcccgc ggagacccug     1560

cacggcacgg ucaccgugga agugcaguau gccgggacag acggaccgug caagguuccu     1620

gcucaaaugg ccgucgauau gcagacccug acacccgugg gccgccucau caccgcuaac     1680

ccugugauua cggaguccac agaaaacagc aaaaugaugc uggagcuuga cccucccuuu     1740

ggggauucuu acaucgucau uggcguggga gacaagaaaa ucacccacca uuggcaccgg     1800

uccggcagca caauugggaa ggccuucgag gcgaccguua gaggagccaa acgcauggcu     1860

gugcugggcg auaccgccug ggacuucggg uccgucggag gcguguuuaa uucucucggg     1920

aagggcaucc aucagaucuu cggagcugcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uucugaucgg aacacuucuc guuuggcugg gccugaacac gaaaaauggg     2040

agcauuuccc ucaccugucu ggcccuugga ggcgugauga ucuuccugag cacagcuguc     2100

ucugcggaug uggggugcuc cguugacuuu agcaag                               2136


<210>  2351
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long CDS opt

<400>  2351
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aaaucuggga      180

gugaacaaau gccacgucca gaucauggau cucggccaua ugugugacgc uaccaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagcccgacg auguggacug uuggugcaau      300

acaaccucua cauggguggu cuacggaacc ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cggugacgcu uccgucccac ucuaccagaa agcugcaaac acggagccag      420

accuggcucg agucccgcga guauacaaag caucugauua aaguugaaaa cuggaucuuu      480

agaaaucccg gcuucgcccu ggcugccgug gcuauugccu ggcuccuggg gagcucuacc      540

ucccagaagg ucaucuaccu ugugaugauu cugcucaucg cgccugccua uagcauucgg      600

ugcaucggag uuucuaaccg cgauuuugug gagggcaugu ccgggggaac gugggucgac      660

guggugcugg aacacggcgg gugugucaca guuauggcuc aggauaaacc caccguggac      720

aucgagcuug ugacaaccac cgucagcaau auggccgagg ugagaucuua cugcuacgag      780

gcuuccauua gcgauauggc cuccgacucu cgguguccua cacaaggaga agccuaucug      840

gacaagcaga gcgauacgca guacguuugc aaaagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguucgg caaggggucc cuggucacau gcgcuaaguu uaccugcagc      960

aaaaagauga caggaaaauc uauccagccc gaaaaucucg aguaucggau uaugcugucc     1020

gugcacggca gccaacauuc ugggaugauc guuaacgaug aaaauagagc gaagguggag     1080

gucaccccua acagcccgcg cgccgaggcu acgcuuggag gcuucggcuc ccuggggcuc     1140

gacugugaac cccggacagg acuggauuuu ucugaccuuu acuaucugac caugaauaac     1200

aaacacuggc ucgugcauaa ggagugguuc cacgauauuc cucugcccug gcacgccggc     1260

gccgacaccg ggacaccuca uuggaauaac aaagaagcuc uggucgaguu uaaggaugcc     1320

cacgcgaaga gacagaccgu gguugugcuc ggaagccagg aaggcgcugu ccauacagcc     1380

cuggccgggg cucuugaggc cgagauggac ggcgcuaaag gacgccuguu cuccggccac     1440

cucaagugcc ggcugaaaau ggauaaacuu agacugaagg gggugagcua cucucucugu     1500

acggccgcgu uuaccuucac aaagguuccc gccgaaaccc ugcauggaac agugaccguc     1560

gaggugcagu augcuggcac ggacgggccg ugcaaggugc cugcccaaau ggcuguugac     1620

augcagaccc ugacacccgu cggacgccuc aucaccgcca acccugugau cacagaaucc     1680

accgagaaua gcaaaaugau gcuggaacuu gauccucccu uuggcgacuc uuacauugug     1740

aucggggucg gcgauaagaa aauuacgcac cacuggcauc gguccggaag cacaaucggc     1800

aaggcguucg aggccaccgu gagaggggcu aaacgcaugg ccguucuggg agacacagcu     1860

ugggauuucg gcuccgucgg gggaguguuu aacucucucg gcaaggggau ucaccagauc     1920

uucggcgccg ccuuuaagag ccuguucgga ggcauguccu gguuuucuca aauccugauu     1980

gggacccuuc ucguguggcu gggacugaau accaaaaacg gcagcaucuc ccucacaugu     2040

cuggcucuug ggggaguuau gauuuuccug agcacggcgg ugucugccga cgucggcugc     2100

uccguggauu uuagcaag                                                   2118


<210>  2352
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long CDS opt

<400>  2352
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

gugggacugc uccugacaac ggccauggcg gcugaaauca cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauua gcuucgccac aacccuggga      180

gucaauaaau gccacguuca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaaggggug gagcccgacg auguggacug uuggugcaac      300

accacaucua ccugggucgu guacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cgguuacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauca aaguggaaaa uuggauuuuu      480

agaaaccccg gcuucgcccu ggucgcugug gccaucgcuu ggcuccuggg gagcucuacg      540

ucccagaagg uuauuuaccu ugugaugauc cugcucauug ccccugcgua uagcauccgg      600

ugcauuggag ucucuaaucg cgauuuugug gagggcaugu ccgggggaac auggguggac      660

gucguucugg aacacggcgg gugugugacc gugauggccc aggauaaacc cacagucgac      720

aucgagcuug ugaccaccac aguuagcaac auggcugagg ugagaucuua cugcuacgaa      780

gccuccauca gcgauauggc uuccgacucu cgguguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgucugc aaaagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cugguuaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauucagccc gaaaaccucg aguaucggau caugcugucc     1020

gugcacggca gccaacauuc ugggaugauu gucaaugaua cgggacacga aacagacgag     1080

aacagagcua agguggaggu caccccuaau agcccgcgcg cggaagccac ccuuggcggc     1140

uucggguccc ugggacucga uugugagccc cggacaggcc uggacuuuuc ugaucuuuac     1200

uaucugacca ugaacaauaa acauuggcuc gugcacaagg aaugguucca cgacaucccu     1260

cugcccuggc augcuggggc cgauacagga acgccucacu ggaacaauaa agaggcccug     1320

guugaauuua aggacgcuca ugccaagaga cagaccgugg ucgugcucgg cagccaggag     1380

ggggcgguuc acacagcucu ggccggcgcc cuugaggcug aaauggaugg agccaaaggc     1440

cgccuguucu ccgggcaucu caagugccgg cugaaaaugg acaaacuuag acugaaggga     1500

gugagcuacu cucucuguac cgcugccuuu acauucacca agguccccgc ggagacgcug     1560

cacggcaccg ugacagugga aguucaguau gccgggaccg acggaccgug caaggucccu     1620

gcucaaaugg ccguggauau gcagacacug acccccgugg gccgccucau uacggcuaac     1680

ccugucauca cagaguccac cgaaaacagc aaaaugaugc uggagcuuga cccucccuuu     1740

ggggauucuu acaucgugau uggcguugga gacaagaaaa ucacacacca uuggcaccgg     1800

uccggcagca ccauugggaa ggccuucgag gcgaccguga gaggagccaa acgcauggcu     1860

guccugggcg auacagccug ggacuucggg uccgugggag gcguuuuuaa uucucucggg     1920

aagggcaucc aucagauuuu cggagcugcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccugaucgg aacgcuucuc guguggcugg gccugaacac caaaaauggg     2040

agcauuuccc ucacaugucu ggcccuugga ggcgucauga ucuuccugag caccgcugug     2100

ucugcggaug uugggugcuc cguggacuuu agcaag                               2136


<210>  2353
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long CDS opt

<400>  2353
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

gugggacugc uccugacaac ggccauggcg gcugaaauca cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauua gcuucgccac aacccuggga      180

gucaauaaau gccacguuca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaaggggug gagcccgacg auguggacug uuggugcaac      300

accacaucua ccugggucgu guacggaacg ugucaacaca agaaaggcga agcccgcaga      360

agccggcgcg cgguuacccu uccgucccau ucuacaagaa agcugcagac ccggagccag      420

acauggcucg agucccgcga guauaccaag caccugauca aaguggaaaa uuggauuuuu      480

agaaaccccg gcuucgcccu ggucgcugug gccaucgcuu ggcuccuggg gagcucuacg      540

ucccagaagg uuauuuaccu ugugaugauc cugcucauug ccccugcgua uagcauccgg      600

ugcauuggag ucucuaaucg cgauuuugug gagggcaugu ccgggggaac auggguggac      660

gucguucugg aacauggcgg gugugugacc gugauggccc aagauaaacc cacagucgac      720

aucgagcuug ugaccaccac aguuagcaac auggcugagg ugagaucuua cugcuacgaa      780

gccuccauca gcgauauggc uuccgacucu cgguguccua cgcagggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgucugc aaaagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cugguuaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauucaaccc gaaaaccucg aguaucggau caugcugucc     1020

gugcacggca gccagcacuc ugggaugauu gucaaugaua ucggacauga aacggacgag     1080

aacagagcua agguggaggu cacaccuaau agcccgcgcg cggaagccac ccuuggcggc     1140

uucggguccc ugggacucga uugugagccc cggaccggcc uggacuuuuc ugaucuuuac     1200

uaucugacaa ugaacaauaa acacuggcuc gugcauaagg aaugguucca cgacauuccu     1260

cugcccuggc acgcuggggc cgauaccgga acaccucauu ggaacaauaa agaggcccug     1320

guugaauuua aggacgcuca cgccaagaga cagacggugg ucgugcucgg cagccaggag     1380

ggggcgguuc auaccgcucu ggccggcgcc cuugaggcug aaauggaugg agccaaaggc     1440

cgccuguucu ccgggcaccu caagugccgg cugaaaaugg acaaacuuag acugaaagga     1500

gugagcuacu cucucuguac agcugucugc accgccgcga aggugcccgc cgagacacug     1560

cauggcaccg ugacgguuga aguccaauau gcugggaccg acggaccgug uaaggugccu     1620

gcccagaugg cuguggauau gcagacacug acccccgucg gccgccucau cacagccaac     1680

ccugugauca ccgaguccac ggaaaacagc aaaaugaugc uggagcuuga cccucccuuu     1740

ggggauucuu acauuguuau cggcguggga gacaagaaaa uuacacacca cuggcaucgg     1800

uccggcagca ccaucgggaa ggcguucgag gccacaguca gaggagcuaa acgcauggcc     1860

gugcugggcg auaccgcuug ggacuuuggg uccguuggag gcguguucaa uucucucggg     1920

aagggcauuc accaaaucuu cggagccgcc uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaga uccugauugg aacccuucuc gucuggcugg gccugaacac aaagaauggg     2040

agcaucuccc ucacgugccu ggcucuugga ggcgugauga uuuuccugag caccgcgguu     2100

ucugccgaug ugggguguuc cgucgacuuu agcaag                               2136


<210>  2354
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long CDS opt

<400>  2354
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacgccag caucgggauu       60

gugucccugc uccugaccac agucauggcg gcugaaauca cgcgcagagg aucugccuac      120

uauauguacc uugaucggag cgacgccggc aaggcuauuu ccuucguuac cacacugggg      180

gugaauaaau gccacgugca gaucauggau cucggacaua ugugugacgc caccaugucu      240

uaugagugcc ccaugcugga ugaaggcguc gagcccgacg auguggacug uuggugcaac      300

acaaccagca cauggguugu guacgggacc ugucaccaua agaaaggaga agcucgcaga      360

ucccggcgcg ccgucacgcu uccgucucac agcaccagaa agcugcaaac acggucccag      420

accuggcucg agagccgcga guauacaaag caucugauca aaguggaaaa uuggauuuuu      480

agaaaccccg gcuucgcgcu gguugccgug gcuaucgccu ggcuccuggg aucuuccacc      540

agccagaagg ucauuuaccu ugugaugauc cugcucauug cuccugccua uucuauccgg      600

ugcauugggg uguccaaucg cgauuuuguc gagggaauga gcggcgggac guggguugac      660

guggugcugg aacacggagg cugugucaca gugauggcgc aggauaaacc caccguugac      720

aucgagcuug ugacaaccac cgucucuaac auggccgagg ugagauccua cugcuacgaa      780

gcuagcaucu ccgauauggc cucugacagc cgguguccua cacaagggga ggcuuaucug      840

gacaagcagu ccgauacgca guacguuugc aaaagaaccc ucguggaccg cggauggggc      900

aauggcugug ggcuguucgg aaagggcagc cuggucacau gcgccaaguu uaccugcucu      960

aaaaagauga cagggaaauc cauucagccc gaaaaccucg aguaucggau caugcugagc     1020

gugcacggau cucaacauag cggcaugauu gucaaugaug aaaacagagc caagguggag     1080

guuaccccua auuccccgcg cgcugaggcg acgcuugggg gauucggcuc ucugggccuc     1140

gacugugaac cccggacagg gcuggauuuu agcgaccuuu acuaucugac caugaacaau     1200

aaacacuggc ucgugcauaa ggagugguuc cacgauaucc cucugcccug gcacuccgga     1260

gccgacaccg aaacaccuca uuggaacaau aaagaggcuc uggucgaauu uaaggaugcc     1320

cacgccaaga gacagaccgu gguugugcuc ggcagccagg agggggcugu ccauacagcc     1380

cuggcgggag cucuugaggc cgaaauggac ggcgccaaag ggcgccuguc uuccggccac     1440

cucaagugcc ggcugaaaau ggauaaacuu agacugaaag gagugagcua cucucucugu     1500

acggcugccu ucaccuuuac aaaggugccc gcugagaccc ugcauggcac aguuaccguc     1560

gaagugcagu augccgggcg cgacggaccg ugcaaggugc cugcgcaaau ggccgucgac     1620

augcagacgc ugacccccgu gggccggcuc auuacagcua acccuguuau caccgagucc     1680

acagaaaaca gcaaaaugau gcuggagcuu gauccucccu ucggggacuc cuacaucgug     1740

auuggagucg gcgauaagaa aaucacccac cacuggcaua gaucugggag cauuaucggc     1800

aaggccuuug aggcuacggu gcgcggagcc aaacggaugg cgguucuggg cgacacagcc     1860

ugggauuucg gguccguggg aggcgucuuc aauucucucg ggaagggcau ucaccagauc     1920

uuuggcgcug ccuucaagag ccuguuuggg ggcauguccu gguucagcca aauccugauu     1980

ggaacccuuc ucguguggcu gggccugaac acaaagaaug ggucuaucuc ccucaccugu     2040

cuggcucuug gaggcguuau gauuuuucug agcaccgccg ugucugccga cgucgggugc     2100

uccguggauu ucagcaag                                                   2118


<210>  2355
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long CDS opt

<400>  2355
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

gugggacugc uccugacaac ggccauggcg gcugaaauca cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauua gcuucgccac aacccuggga      180

gucaauaaau gccacguuca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaaggggug gagcccgacg auguggacug uuggugcaac      300

accacaucua ccugggucgu guacggaacg ugucaccaua agaaaggcga agcccgcaga      360

agccggcgcg cgguuacccu uccgucccac ucuacaagaa agcugcaaac ccggagccag      420

acauggcucg agucccgcga guauaccaag caucugauca aaguggaaaa uuggauuuuu      480

agaaaccccg gcuucgcccu ggucgcugug gccaucgcuu ggcuccuggg gagcucuacg      540

ucccagaagg uuauuuaccu ugugaugauc cugcucauug ccccugcgua uagcauccgg      600

ugcauuggag ucucuaaucg cgauuuugug gagggcaugu ccgggggaac auggguggac      660

gucguucugg aacacggcgg gugugugacc gugauggccc aggauaaacc cacagucgac      720

aucgagcuug ugaccaccac aguuagcaac auggcugagg ugagaucuua cugcuacgaa      780

gccuccauca gcgauauggc uuccgacucu cgguguccua cgcaaggaga ggccuaucug      840

gacaagcaga gcgauaccca guacgucugc aaaagaacac ucguggaccg cggcuggggc      900

aaugggugug gacuguucgg caaggggucc cugguuaccu gcgccaaguu uacaugcagc      960

aaaaagauga ccggaaaauc uauucagccc gaaaaccucg aguaucggau caugcugucc     1020

gugcacggca gccaacauuc ugggaugauu gucaaugaug aaaacagagc uaagguggag     1080

gucacgccua auagcccgcg cgcggaggcc acacuuggag gcuucggcuc ccuggggcuc     1140

gacugugaac cccggaccgg acuggauuuu ucugaccuuu acuaucugac caugaacaau     1200

aaacacuggc ucgugcauaa ggagugguuc cacgauaucc cucugcccug gcacgcuggc     1260

gccgacacag ggaccccuca uuggaacaau aaagaagccc ugguugaguu uaaggaugcu     1320

cacgccaaga gacagacagu ggucgugcuc ggaagccagg aaggcgcggu ucauacggcu     1380

cuggccgggg cccuugaggc ugagauggac ggcgccaaag gacgccuguu cuccggccac     1440

cucaagugcc ggcugaaaau ggauaaacuu agacugaaag gggugagcua cucucucugu     1500

accgcugccu uuacauucac caaggucccc gcggaaacac ugcauggaac cgugacggug     1560

gagguucagu augccggcac cgacgggccg ugcaaggucc cugcucaaau ggccguggac     1620

augcagacac ugacccccgu gggacgccuc auuacagcua acccugucau caccgaaucc     1680

acggagaaca gcaaaaugau gcuggaacuu gauccucccu uuggcgacuc uuacaucgug     1740

auugggguug gcgauaagaa aaucacacac cacuggcauc gguccggaag caccauuggc     1800

aaggccuucg aggcgacagu gagaggggcc aaacgcaugg cuguccuggg agacaccgcc     1860

ugggauuucg gcuccguugg gggaguguuu aauucucucg gcaaggggau ccaccagauu     1920

uucggcgcug ccuuuaagag ccuguucgga ggcauguccu gguuuucuca aauccugauc     1980

gggacccuuc ucguguggcu gggacugaac acaaagaaug gcagcauuuc ccucacgugu     2040

cuggcccuug ggggagucau gaucuuccug agcaccgcug ugucugcgga cguuggcugc     2100

uccguggauu uuagcaag                                                   2118


<210>  2356
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long CDS opt

<400>  2356
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aacccuggga      180

gugaauaaau gccacgucca gaucauggau cucggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcugga ugaagggguu gagccugacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccaua agaaaggcga aacccgcaga      360

agccggcgcu ccgugucucu uagauaucac uacacacgga agcugcaaac ccgcagccag      420

acauggcucg aguccagaga guauaagaaa caucugauua ugguugaaaa uuggaucuuu      480

cggaacccgg gcuucgccau ugugagcguc gcgaucaccu ggcugauggg gucucucacg      540

ucccagaagg ugauuuaccu gguuaugauc gugcuuauug uccccgccua cagcaucucu      600

ugcaucggag uguccaaucg cgaucuggug gagggcauga gcgggggaac augggucgac      660

guugugcucg aacacggcgg gugugugacc gagauggcuc aggauaaacc uacagucgac      720

auugagcugg ugaccaugac cguuucuaac auggccgaag ugagauccua uugcuacgag      780

gcuagccuuu ccgauauggc cucugcgagc cgguguccca cacaaggaga accuucccug      840

gacaagcaga gcgacacgca gucugucugc aaaagaaccc ucggcgaucg cggcuggggg      900

aauggaugug gcaucuuugg gaagggaucc cuggugacau gcagcaaguu uaccugcugu      960

aaaaagaugc ccggcaaauc uauucagccu gagaaccugg aauaucggau caugcucccg     1020

guucacggga gccaacauuc cggaaugauu gugaaugaca ucggccacga gacagaugag     1080

aacagagcca aggucgaagu gacccccaau ucuccucgcg cugaggccac gcugggcggg     1140

uuuggaagcc uuggccugga cugcgaaccc cggacagggc ucgauuucuc cgaccuguac     1200

uaucuuacca ugaacaauaa acauuggcug guccacaagg agugguuuca cgauaucccu     1260

cuccccuggc augcuggagc cgacaccggc acaccgcacu ggaacaauaa agaagcccug     1320

guggaguuca aggaugcuca ugcgaagaga cagaccguug ugguccuggg gagccaggag     1380

ggcgccgugc acacagcucu cgccggagcc cuggaagcug agauggacgg cgccaaaggg     1440

cgccuuuucu cuggacaucu gaagugucgg cucaaaaugg auaagcugag acuuaaaggc     1500

guuuccuaca gccugugcac ggcggcuuuc accuuuacaa aggugccugc cgaaacccuc     1560

cacgggacag ucaccgugga ggugcagucu gccggaacgg acggccccug uaagguuccu     1620

gcucaaaugg ccgucgacau gcagacccug acacccgugg ggcgccugau uaccgcuaac     1680

cccgugauca cagaauccac cgagaacagc aaaaugaugc ucgagcugga uccuccguuc     1740

ggcgacuccu auauugucau cggagugggc gauaagaaaa uuacgcacca uuggcaccgg     1800

ucugggagca caaucggaaa ggccuuugaa gcgaccguua gaggcgccaa acgcauggcu     1860

gugcuugggg acacagccug ggauuucgga uccgucggcg ggguguucaa uucucugggc     1920

aagggaaucc aucagauuuu uggcgcugcc uucaagagcc ucuuuggggg aauguccugg     1980

uucagccaaa uccugauugg cacccugcuu guuuggcucg ggcugaacac caaaaaugga     2040

ucuaucuccc ugacaugccu cgcgcugggc ggggugauga uuuuucuuag cacggccguc     2100

ucugcugacg ugggcuguuc cguugauuuc agcaag                               2136


<210>  2357
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long CDS opt

<400>  2357
agaaucauua acgcccggaa ggagcgcaaa agacggggcg cugacaccag caucgggauu       60

aucggacugc uccugacaac ggccauggcg gcugaaauua cccgcagagg cuccgccuac      120

uauauguacc uugaucgguc ugacgccggg aaggcuauca gcuucgccac aaccauggga      180

gugaauaaau gccacgucca gaucauggau cugggccaua ugugugacgc uacaaugucc      240

uaugagugcc ccaugcucga ugaagggguu gagccugacg auguggacug uuggugcaac      300

accacaucua ccuggguggu cuacggaacg ugucaccaua agaaaggcga agcccgcccg      360

agcagacggg cggugacccu gcccucccac ucuacacgca aacuucaaac cagaagccag      420

acauggcugg agucccggga guauaccaag caucucauua agguugaaaa uuggauccug      480

cgcaacccug gcuuuacggu ggccgcucug gccauggcuu ggcuccuggg gaauagcaca      540

ucucagagag ucauuuaccu ugugaugauc cugcucauug cccccgcgua uuccauccgg      600

ugcauuggag uuagcaaccg cgauuucgug gagggcaugu cugggggaac cugggucgac      660

guggugcugg aacacggcgg gugugucaca guuauggccc aggauaagcc uaccguggac      720

aucgagcuug ugaccacaac ggucuccaau auggcugagg ugagaagcua cugcuacgaa      780

gccucuaucu ccgauauggc uagcgacucc cgguguccca cccaaggaga ggccuaucug      840

gacaaacagu cugauacaca guacguuugc aagagaaccc ucguggaccg cggcuggggc      900

aacgggugug gacuguuugg caaagggagc cuggucacau gcgccaaguu uaccugcucc      960

aagaaaauga cgggaaagag cauucagccu gaaaaucucg aguaucggau caugcugucu     1020

gugcacggcu cccaacauag cgggaugauu guuaacgaua ucggacacga aacagacgag     1080

aauagagcua aaguggaggu caccccgaac ucuccccgcg cggaagccac ccuuggcggc     1140

uucgggagcc ugggacucga uugugagccu cggacaggcc uggacuucuc cgaucuuuac     1200

uaucugacca ugaauaacaa gcauuggcuc gugcacaaag aaugguuuca cgacauuccc     1260

cugccuuggc augcuggggc cgauacagga acgccccacu ggaauaacaa ggaggcccug     1320

gucgaguuca aagacgcuca ugccaagaga cagaccgugg uugugcucgg cucucaggaa     1380

ggggcggucc acacagcucu ggccggcgcc cuugaggcug aaauggaugg agccaagggc     1440

cgccuguuua gcgggcaucu caaaugccgg aucaagaugg acaaacugag acuuaaggga     1500

guguccuaca gccuguguac cgcugccuuc acauuuucua aaguuccggc ggagacccuc     1560

cacggcacgg ugaccgucga agugcaguau gccgggcgcg acggaccuug caaggugccc     1620

gcucaaaugg ccguugauau gcagacacug accccugucg gccggcugau cacagcuaac     1680

cccauuauca ccgaguccac ggaaaauagc agaaugaugc ucgagcugga ucccccuuuc     1740

ggggacucuu acauugugau cggcguggga gacaagaaaa uuacacacca uuggcaccgc     1800

uccggcagca ccaucgggaa ggccuuugag gcgacagucc ggggagccaa aagaauggcu     1860

gugcuuggcg auaccgccug ggacuucggg uccguuggag gcguguucaa cucucugggg     1920

aagggcgucc aucagguguu uggagcugcc uucaaaagcc ucuuuggcgg gauguccugg     1980

uucucucaaa uccugauugg aacccugcuu guuuggcucg gccugaauac aaagaacggg     2040

agcaucuccc uggccugucu cgcucuggga ggcgcgauga uuuuucuuag cacggccgug     2100

ucugcugaug ucgggugcuc cguggacuuc                                      2130


<210>  2358
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short CDS opt

<400>  2358
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauauugu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc c                                               2061


<210>  2359
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short CDS opt

<400>  2359
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacacaugu      180

gaugccacca ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacacaac cuccacgugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acacuuccca gccauuccac ccggaaacug      360

caaacacgcu cucagaccug gcucgagagc agagaguaca cgaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacaucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggaccuggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcgcaggac      660

aagccuaccg uggauauuga gcuugugacc acaacggucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacccaa      780

ggggaggcuu accuggacaa gcagucugac acacaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu cacaugcgcc      900

aaauuugccu gcuccaagaa aaugaccggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacacgggg     1020

caugaaacag augagaaccg ggcuaaggug gagauuaccc ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac aggacuggau     1140

uuuuccgacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca cagggacgcc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac cgucgugguu     1320

cucggaucuc aggagggcgc ggugcauaca gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguaccgcug ccuucacauu uaccaagauu     1500

ccggcggaga cgcugcaugg aaccgugaca guugaagugc aguacgccgg caccgacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacgg cuaaccccgu uauuacagag uccaccgaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauaca gccugggacu ucggcuccgu ugggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacgc uccugaugug gcugggacuc     1980

aacgcuaaga auggcagcau cucccugaug ugucuugcgc uugggggagu gcucauuuuc     2040

cugagcaccg ccgugucugc u                                               2061


<210>  2360
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short CDS opt

<400>  2360
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gaucaagguu      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uucgcugcau cggggugucu aacagagacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcac ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgg      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaaag aacacucguc      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcauuaugc uguccguuca cggaagccaa cauucuggca ugaucgggua cgagacggac     1020

gaagauagag ccaaggugga ggucacaccu aacagcccgc gggccgaggc uacccuugga     1080

ggcuucggcu cccuggggcu cgacugugaa cccagaaccg gacuggauuu uucugaccuu     1140

uauuaccuga caaugaauaa caaacacugg cucgugcaua aggagugguu ccacgauauu     1200

ccucugcccu ggcacgcggg cgccgacacc gggacaccuc auuggaauaa caaagaagcu     1260

cuggucgagu uuaaggaugc ccacgccaaa cgccagacgg ugguugugcu cggaagccag     1320

gaaggcgcug uccauaccgc ccuggcgggg gcucuugagg ccgagaugga cggcgccaag     1380

ggacggcugu ucuccggcca ccucaagugc agacugaaaa uggauaaacu ucgccugaag     1440

ggggugagcu auucucucug uacagcugcc uuuaccuuca caaagguucc cgcugaaacc     1500

cugcauggaa cggugaccgu cgaggugcag uacgccggca cagacgggcc gugcaaaauc     1560

ccugugcaaa uggcgguuga caugcagacc cugacacccg ucggacggcu cauuaccgcc     1620

aauccuguga ucacggaauc cacagagaac agcaagauga ugcuggaacu ugauccuccc     1680

uuuggcgacu cuuacaucgu gauugggguc ggcgauaaga aaaucaccca ccacuggcau     1740

agauccggaa gcacaauugg caaggcuuuc gaggccaccg ugcgcggggc uaaacggaug     1800

gccguucugg gagacaccgc gugggauuuc ggcuccgucg ggggaguguu uaauucucuc     1860

ggcaagggga uccaccagau uuucggcgcc gcuuuuaaaa gccuguucgg aggcaugucc     1920

ugguuuucuc aaauccugau cgggacacuu cucguguggc ugggacugaa cacgaagaac     1980

ggcagcauuu cccucaccug ucuggcccuu gggggaguua ugaucuuccu gagcacagcu     2040

gugucugcc                                                             2049


<210>  2361
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short CDS opt

<400>  2361
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2362
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short CDS opt

<400>  2362
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gaucaagguu      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uucgcugcau cggggugucu aacagagacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcac ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgg      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaaag aacacucguc      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcauuaugc uguccguuca cggaagccaa cauucuggca ugaucgugaa cgacacgggg     1020

uacgagacag augaaaauag agccaagguc gaggugaccc cuaacagccc gcgggccgag     1080

gcuacccuug gaggcuucgg cucccugggg cucgacugug aacccagaac aggacuggau     1140

uuuucugacc uuuauuaccu gaccaugaau aacaaacacu ggcucgucca uaaggagugg     1200

uuccacgaua uuccucugcc cuggcacgcg ggcgccgaca cagggacgcc ucauuggaau     1260

aacaaagaag cucuggugga guuuaaggau gcccacgcca aacgccagac cguugugguc     1320

cucggaagcc aggaaggcgc ugugcauaca gcccuggcgg gggcucuuga ggccgagaug     1380

gacggcgcca agggaaagcu guucuccggc caccucaaau gccggcugaa gauggauaaa     1440

cuuagacuga aggggguuag cuauucucuc uguaccgcug ccuuuacauu caccaaagug     1500

cccgcugaaa cgcugcaugg aaccgucaca guggaggugc aguacgccgg caccgacggg     1560

ccgugcaaga ucccuguuca aauggcgguc gacaugcaga cacugacccc cgugggacgc     1620

cucauuacgg ccaauccugu gaucacagaa uccaccgaga acagcaagau gaugcuggaa     1680

cuugauccuc ccuuuggcga cucuuacauc gucauugggg ugggcgauaa aaagaucaca     1740

caccacuggc aucgguccgg aagcaccauu ggcaaggcuu ucgaggccac cguuagaggg     1800

gcuaaacgca uggccgugcu gggagacaca gcgugggauu ucggcuccgu cgggggagug     1860

uuuaacucuc ucggcaaagg gauccaccag auuuucggcg ccgcuuuuaa gagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug aucgggacgc uucucguuug gcugggacug     1980

aauaccaaga acggcagcau uucccucaca ugucuggccc uugggggagu gaugaucuuc     2040

cugagcaccg cugucucugc c                                               2061


<210>  2363
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short CDS opt

<400>  2363
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca cagaaagaaa      300

ggagaggccc ggcgcagcag acgggcugug acccuuccgu cccauucuac acgcaagcug      360

caaaccagaa gccagacaug gcucgaaucc cgggaguaua ccaaacaccu gaucaagguu      420

gagaacugga ucuuucgcaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uuagaugcau cggggugucu aaccgggacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcau ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgc      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucuagaug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaacg gacacucguc      840

gaucgcggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

agaauuaugc uguccguuca cggaagccaa cacucuggca ugaucgugaa cgacacgggg     1020

uacgagacag augaaaaucg ggccaagguc gaggugaccc cuaacagccc gagagccgag     1080

gcuacccuug gaggcuucgg cucccugggg cucgacugug aaccccgcac aggacuggau     1140

uuuucugacc uuuauuaccu gaccaugaau aacaaacauu ggcucgucca caaggagugg     1200

uuccaugaua uuccucugcc cuggcacgcg ggcgccgaca cagggacgcc ucacuggaau     1260

aacaaagaag cucuggugga guuuaaggau gcccaugcca aacggcagac cguugugguc     1320

cucggaagcc aggaaggcgc ugugcacaca gcccuggcgg gggcucuuga ggccgagaug     1380

gacggcgcca agggaaagcu guucuccggc caucucaaau gcagacugaa gauggauaaa     1440

cuucgccuga aggggguuag cuauucucuc uguaccgcug ccuuuacauu caccaaagug     1500

cccgcugaaa cgcugcacgg aaccgucaca guggaggugc aguacgccgg caccgacggg     1560

ccgugcaaga ucccuguuca aauggcgguc gacaugcaga cacugacccc cgugggacgg     1620

cucauuacgg ccaauccugu gaucacagaa uccaccgaga acagcaagau gaugcuggaa     1680

cuugauccuc ccuuuggcga cucuuacauc gucauugggg ugggcgauaa aaagaucaca     1740

caucacuggc acagauccgg aagcaccauu ggcaaggcuu ucgaggccac cguucgcggg     1800

gcuaaacgga uggccgugcu gggagacaca gcgugggauu ucggcuccgu cgggggagug     1860

uuuaacucuc ucggcaaagg gauccaucag auuuucggcg ccgcuuuuaa gagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug aucgggacgc uucucguuug gcugggacug     1980

aauaccaaga acggcagcau uucccucaca ugucuggccc uugggggagu gaugaucuuc     2040

cugagcaccg cugucucugc c                                               2061


<210>  2364
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short CDS opt

<400>  2364
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gaucaagguu      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uucgcugcau cggggugucu aacagagacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcac ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgg      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaaag aacacucguc      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcauuaugc uguccguuca cggaagccaa cauucuggca ugacggugaa cgacaucggg     1020

uacgagacag augaaaauag agccaagguc gaggugaccc cuaacagccc gcgggccgag     1080

gcuacccuug gaggcuucgg cucccugggg cucgacugug aacccagaac aggacuggau     1140

uuuucugacc uuuauuaccu gaccaugaau aacaaacacu ggcucgucca uaaggagugg     1200

uuccacgaua uuccucugcc cuggcacgcg ggcgccgaca cagggacgcc ucauuggaau     1260

aacaaagaag cucuggugga guuuaaggau gcccacgcca aacgccagac cguugugguc     1320

cucggaagcc aggaaggcgc ugugcauaca gcccuggcgg gggcucuuga ggccgagaug     1380

gacggcgcca agggaaagcu guucuccggc caccucaaau gccggcugaa gauggauaaa     1440

cuuagacuga aggggguuag cuauucucuc uguaccgcug ccuuuacauu caccaaagug     1500

cccgcugaaa cgcugcaugg aaccgucaca guggaggugc aguacgccgg caccgacggg     1560

ccgugcaaga ucccuguuca aauggcgguc gacaugcaga cacugacccc cgugggacgc     1620

cucauuacgg ccaauccugu gaucacagaa uccaccgaga acagcaagau gaugcuggaa     1680

cuugauccuc ccuuuggcga cucuuacauc gucauugggg ugggcgauaa aaagaucaca     1740

caccacuggc aucgguccgg aagcaccauu ggcaaggcuu ucgaggccac cguuagaggg     1800

gcuaaacgca uggccgugcu gggagacaca gcgugggauu ucggcuccgu cgggggagug     1860

uuuaacucuc ucggcaaagg gauccaccag auuuucggcg ccgcuuuuaa gagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug aucgggacgc uucucguuug gcugggacug     1980

aauaccaaga acggcagcau uucccucaca ugucuggccc uugggggagu gaugaucuuc     2040

cugagcaccg cugucucugc c                                               2061


<210>  2365
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short CDS opt

<400>  2365
accagcaucg gcauuguggg gcugcuccug acaacggcca uggcugccga gaucaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc cauuagcuuc      120

gccacaaccc ugggggucaa caaaugccac guucagauca uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguggaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcuguu acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gauuaaggug      420

gagaacugga ucuuucggaa ucccggcuuc acgcuggucg ccguggcgau cgccuggcuc      480

cugggcagcu cuacauccca gaagguuauu uaccuuguga ugauccugcu cauugcuccu      540

gccuauagca uccgcugcau uggggucucu aacagagacu uuguggaagg aauguccggc      600

gggaccuggg uggaugucgu ucuggagcac ggaggcugug ugacagugau ggcucaggac      660

aaacccaccg ucgauaucga acuugugacc acaacgguua gcaauauggc cgaggugcgg      720

ucuuacugcu acgaggcguc cauuagcgac auggccuccg auucucgcug uccuacccaa      780

ggggaggcuu aucuggacaa gcagagcgac acacaguacg ucugcaaaag aacccucgug      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu uacaugcgcc      900

aaauuuaccu gcagcaagaa gaugacgggg aaaucuaucc agcccgaaaa ucucgaauau      960

cgcaucaugc uguccgugca cggaagccaa cauucuggca ugauugucaa cgacgagaau     1020

agagcuaagg uggaagucac accuaacagc ccgcgggccg aggccacccu ugggggauuc     1080

ggcucccugg gccucgauug ugagcccaga accgggcugg acuuuucuga ucuuuacuau     1140

cugacaauga auaacaaaca cuggcucgug cauaaggaau gguuccacga caucccucug     1200

cccuggcacg cuggagcgga uaccggcaca ccucauugga auaacaaaga ggcccugguu     1260

gaauuuaagg acgcucacgc caaacgccag acgguggucg ugcucgggag ccaggaggga     1320

gccguucaua ccgcucuggc cggcgcgcuu gaagcugaga uggauggggc caagggccgg     1380

cuguucuccg gacaccucaa gugcagacug aaaauggaca aacuucgccu gaagggcgug     1440

agcuacucuc ucuguacagc cgcuuuuacc uucacaaagg uccccgccga gacccugcau     1500

gggacgguga ccguggaagu ucaguaugcu ggaacagaug gcccgugcaa agucccugcc     1560

caaauggcgg uggacaugca gacccugaca cccguggggc ggcucauuac cgccaauccu     1620

gucaucacgg aguccacaga aaacagcaag augaugcugg agcuugaccc ucccuuugga     1680

gauucuuaca uugugaucgg cguuggggac aagaaaauca cccaccacug gcauagaucc     1740

ggcagcacaa uuggaaaggc uuucgaagcc accgugcgcg gcgcuaaacg gauggccguc     1800

cugggggaua ccgcguggga cuucggaucc gugggcgggg uuuuuaacuc ucucggaaag     1860

ggcauccacc agauuuucgg ggccgcuuuu aaaagccugu ucggcggaau guccugguuu     1920

ucucaaaucc ugauuggcac acuucucgug uggcuggggc ugaauacgaa gaacggaagc     1980

aucucccuca ccugucuggc ccuuggcggg gucaugaucu uccugagcac agcugugucu     2040

gcc                                                                   2043


<210>  2366
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short CDS opt

<400>  2366
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gaucaagguu      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uucgcugcau cggggugucu aacagagacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcac ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgg      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaaag aacacucguc      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcauuaugc uguccguuca cggaagccaa cauucuggca ugaucgggua cgagacggac     1020

gaaaacagag ccaaggugga ggucacaccu aauagcccgc gggccgaggc uacccuugga     1080

ggcuucggcu cccuggggcu cgauugugaa cccagaaccg gacuggacuu uucugaucuu     1140

uauuaccuga caaugaacaa uaaacacugg cucgugcaua aggagugguu ccacgacauu     1200

ccucugcccu ggcacgcggg cgccgauacc gggacaccuc auuggaacaa uaaagaagcu     1260

cuggucgagu uuaaggacgc ccacgccaaa cgccagacgg ugguugugcu cggaagccag     1320

gaaggcgcug uccauaccgc ccuggcgggg gcucuugagg ccgagaugga uggcgccaag     1380

ggaaagcugu ucuccggcca ccucaaaugc cggcugaaga uggacaaacu uagacugaag     1440

ggggugagcu auucucucug uacagcugcc uuuaccuuca caaaaguucc cgcugaaacc     1500

cugcauggaa cggugaccgu cgaggugcag uacgccggca cagaugggcc gugcaagauc     1560

ccugugcaaa uggcgguuga caugcagacc cugacacccg ucggacgccu cauuaccgcc     1620

aacccuguga ucacggaauc cacagagaau agcaagauga ugcuggaacu ugacccuccc     1680

uuuggcgauu cuuacaucgu gauugggguc ggcgacaaaa agaucaccca ccacuggcau     1740

cgguccggaa gcacaauugg caaggcuuuc gaggccaccg ugagaggggc uaaacgcaug     1800

gccguucugg gagauaccgc gugggacuuc ggcuccgucg ggggaguguu uaacucucuc     1860

ggcaaaggga uccaccagau uuucggcgcc gcuuuuaaga gccuguucgg aggcaugucc     1920

ugguuuucuc aaauccugau cgggacacuu cucguguggc ugggacugaa cacgaagaau     1980

ggcagcauuu cccucaccug ucuggcccuu gggggaguua ugaucuuccu gagcacagcu     2040

gugucugcc                                                             2049


<210>  2367
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short CDS opt

<400>  2367
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggccgcgg gauucaccag aucuucggcg ccgccuuuaa gagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaaa auggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2368
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short CDS opt

<400>  2368
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggccug gcucucgccg cggccgcuau cgccuggcug      480

cuuggcucca gcacgucuca gaaagucauc uaucugguga ugauucuccu gaucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu ucguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuugaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuggugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accucgacaa gcagucugac acccaguaug uuugcaaacg gacacuggug      840

gaucgcggau ggggcaaugg cugugggcug uuuggaaagg gcagccucgu gaccugcgcc      900

aaauucgccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccuggaguac      960

agaaucaugc uuucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccugg gaggcuuugg cagccucggg cuggacugug agccucgcac cggacuugau     1140

uucuccgacc uguauuaccu cacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuucacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccucgugga guucaaggau gcucacgcca aacggcagac ggucgugguu     1320

cugggaucuc aggaaggcgc ggugcauacc gcucuugccg gggcccugga ggcugaaaug     1380

gacggcgcca agggaagacu cagcuccggc caccugaaau gccgccuuaa gauggauaag     1440

cugcggcuca aaggggucag cuauucucug uguacagcug ccuuuaccuu cacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccucacacc ugugggaaga     1620

cugaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuugag     1680

cuggaucccc cuuuuggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu ucgaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu cggagauacc gccugggacu uuggcuccgu cgggggagcu     1860

cuuaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuucaa aagccuguuu     1920

ggaggcaugu ccugguucuc ucaaauucuc aucgggacac ugcugaugug gcucggacug     1980

aacacgaaga auggcagcau cucccuuaug ugucuggcuc ucgggggagu ucugauuuuu     2040

cugagcaccg cggugucugc c                                               2061


<210>  2369
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short CDS opt

<400>  2369
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggu aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauucaucag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2370
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short CDS opt

<400>  2370
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacacccaga cacugacccc ugugggaaga     1620

cucaucacgg cuaaccccgu uauuacagag uccaccgaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauaca gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacgc uccugaugug gcugggacuc     1980

aacaccaaga auggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcacag cggugucugc c                                               2061


<210>  2371
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short CDS opt

<400>  2371
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgagauccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc c                                               2061


<210>  2372
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short CDS opt

<400>  2372
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacagga aagagcauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cgggucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc cagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccuggcuca aauggccgug gacaugcaga cccucacacc cgugggaaga     1620

cugaucaccg cuaacccugu uauuacggag uccacagaaa acagcaagau gaugcuugag     1680

cuggauccgc ccuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu cggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cuuaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuuuuc     1920

ggaggcaugu ccugguuuuc ucaaauucuc aucgggacac ugcugaugug gcucggacug     1980

aacacgaaga auggcagcau cucccuuaug ugucuggcuc ucgggggagu ucugauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2373
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short CDS opt

<400>  2373
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuacc cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacacaac cuccacgugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acacuuccca gccauuccac ccggaaacug      360

caaacacgcu cucagaccug gcucgagagc agagaguaca cgaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacaucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggaccuggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcgcaggac      660

aagccuaccg uggauauuga gcuugugacc acaacggucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacccaa      780

ggggaggcuu accuggacaa gcagucugac acacaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu cacaugcgcc      900

aaauuugccu gcuccaagaa aaugaccggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacacgggg     1020

caugaaacag augagaaccg ggcuaaggug gagauuaccc ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac aggacuggau     1140

uuuuccgacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca cagggacgcc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac cgucgugguu     1320

cucggaucuc aggagggcgc ggugcauaca gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguaccgcug ccuucacauu uaccaagauu     1500

ccggcggaga cgcugcaugg aaccgugaca guugaagugc aguacgccgg caccgacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacgg cuaaccccgu uauuacagag uccaccgaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauaca gccugggacu ucggcuccgu ugggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacgc uccugaugug gcugggacuc     1980

aacaccaaga auggcagcau cucccugaug ugucuugcuc uugggggagu gcucauuuuc     2040

cugagcacag cggugucugc c                                               2061


<210>  2374
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short CDS opt

<400>  2374
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcaccg uggucucugc g                                               2061


<210>  2375
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short CDS opt

<400>  2375
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccgcuugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcca gacggucucg cagagcuguu acgcuuccca gccauuccac ccggaaacug      360

caaacacgcc cucagaccug gcucgagucu agagaguaca caaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa ccccggcuuc gcccuggcgg ccgcugccau cgcuuggcuc      480

cugggcagcu ccaccagcca gaaagucauc uaucuuguga ugauucugcu caucgccccu      540

gcguacucua uuagaugcau cgggguuucc aaucgggacu uuguggaggg aaugagcggc      600

gggacguggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcccaggac      660

aagcccaccg uggauauuga gcuugugaca accaccgucu cuaacauggc ugaggugcgc      720

uccuacugcu augaagccag caucucugac auggcuuccg auagcagaug uccuacacaa      780

ggggaggccu accuggacaa gcaguccgac acgcaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcucucuggu cacaugcgcc      900

aaauuugcuu gcagcaagaa aaugaccggg aaguccauuc agccggaaaa ccucgaguac      960

agaaucaugc ugagcgugca cggaucucaa cacuccggca ugaucguuaa ugacacaggg     1020

caugaaaccg augagaaccg ggcgaaggug gagauuacgc ccaauagccc uagagccgaa     1080

gcuacacuug gaggcuucgg cucucugggg cucgacugug agccccgcac cggacuggau     1140

uuuagcgacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccucugcc cuggcacgcc ggcgccgaca cagggacccc gcauuggaac     1260

aauaaagagg cucuggugga auuuaaggau gcccacgcga aacggcagac agucgugguu     1320

cucggauccc aggagggcgc ugugcauacg gcccuggccg gggcucuuga ggccgaaaug     1380

gacggcgcua agggaagacu gucuagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucuc cuauagccuc uguaccgccg cguucacauu uaccaagauu     1500

ccugccgaga cacugcaugg aaccgugacg guugaagugc aguacgcugg caccgacggg     1560

cccugcaaag ucccugccca aauggcugug gacaugcaga cacugacccc cgugggaaga     1620

cucaucacag ccaaccccgu uauuaccgag ucuacggaaa acuccaagau gaugcuggag     1680

cuugauccuc cguucggcga cagcuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcucugg auccaccauc ggcaaggcgu uugaagccac agugagaggg     1800

gcuaaacgga uggccguccu gggagauacc gcuugggacu ucggcagcgu ugggggagcc     1860

cucaauuccc ugggcaaggg gauucaccag aucuucggcg ccgcuuuuaa aucucuguuc     1920

ggaggcauga gcugguuuuc ccaaauucuu aucgggaccc uccugaugug gcugggacuc     1980

aacacaaaga auggcucuau cagccugaug ugucuugcgc uugggggagu gcucauuuuc     2040

cuguccacgg ccgugagcgc u                                               2061


<210>  2376
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short CDS opt

<400>  2376
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuaa aaaggucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcag      780

ggggaggcuu accuggacaa acaaucugac acccaguaug uuugcaagcg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaaag gcagccuggu gaccugcgcc      900

aaguuugccu gcuccaaaaa gaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccag cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaagug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaagcacu ggcucgucca uaaagaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaggagg cccuggugga auuuaaagau gcucacgcca agcggcaaac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca aaggaagacu gagcuccggc caccucaagu gccgccugaa gauggauaaa     1440

cuucggcuga agggggucag cuauucucuc uguacagcug ccuucaccuu uacaaaaauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaagg uccccgcuca gauggccgug gacaugcaaa cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaaaau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa gaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaagccu uugaagcgac cgugagaggg     1800

gccaagcgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaagg gauucaccag aucuucggcg ccgccuuuaa gagccuguuc     1920

ggaggcaugu ccugguuuuc ucagauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaaa auggcagcau cucccugaug ugucuugcuc ucgggggagu ucugauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2377
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short CDS opt

<400>  2377
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgagaca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucua uucggugcau cgggguuucc aaucgcgacu uuguggaggg aaugagcggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu cuaacauggc cgaggugaga      720

uccuacugcu augaagcuag caucucugac auggccuccg auagccggug ucccacgcaa      780

ggggaggcuu accuggacaa gcaguccgac acccaguaug uuugcaaacg cacacucgug      840

gauagaggau ggggcaaugg cugugggcug uucggaaagg gcucucuggu gaccugcgcc      900

aaauuugccu gcagcaagaa aaugacaggg aaguccauuc agccugaaaa ccucgaguac      960

cggaucaugc ugagcgugca cggaucucaa cacuccggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaacag agcuaaggug gagauuacac cgaauagccc ccgcgcggaa     1080

gccacccuug gaggcuucgg cucucugggg cucgacugug agccucggac cggacuggau     1140

uuuagcgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aaagacagac ggucgugguu     1320

cucggauccc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggacgccu gucuagcggc caccucaaau gccggcugaa gauggauaag     1440

cugagacuga aaggggucuc cuauagccuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggacgc     1620

cucaucaccg cuaaccccgu uauuacggag ucuacagaaa acuccaagau gaugcuggag     1680

cuugaucccc cuuucggcga cagcuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucggucugg auccacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgca uggcuguccu gggagauacc gccugggacu ucggcagcgu cgggggagcu     1860

cucaauuccc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aucucuguuc     1920

ggaggcauga gcugguuuuc ccaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcucuau cagccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cuguccaccg cggugagcgc c                                               2061


<210>  2378
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short CDS opt

<400>  2378
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaaccc aggagggcgc ggugcauaca gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gucuagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucuc cuauagccuc uguaccgcug ccuucacauu uaccaagauu     1500

ccggcggaga cgcugcaugg aaccgugaca guugaagugc aguacgccgg caccgacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacgg cuaaccccgu uauuacagag ucuaccgaaa acuccaagau gaugcuggag     1680

cuugaucccc cuuucggcga cagcuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcucugg auccaccauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauaca gccugggacu ucggcagcgu cgggggagcu     1860

cucaauuccc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aucucuguuc     1920

ggaggcauga gcugguuuuc ccaaauucuu aucgggacgc uccugaugug gcugggacuc     1980

aacaccaaga auggcucuau cagccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cuguccacag cggugagcgc c                                               2061


<210>  2379
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short CDS opt

<400>  2379
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacuccc ugagaugcau ugggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuugaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauaucga gcuggugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accucgacaa gcagucugac acccaguaug uuugcaaacg gacacuggug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccucgu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccuggaguac      960

agaauuaugc uuucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagaucacac ccaauucucc gagagcggaa     1080

gccacccugg gaggcuucgg cagccucggg cuggacugug agccucgcac cggacuugau     1140

uuuuccgacc uguauuaccu cacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccucgugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cugggaucuc aggagggcgc ggugcauacc gcucuugccg gggcccugga ggcugaaaug     1380

gacggcgcca agggaagacu cagcuccggc caccugaaau gccgccuuaa gauggauaag     1440

cugcggcuca aaggggucag cuauucucug uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccucacacc ugugggaaga     1620

cugauuaccg cuaaccccgu uaucacggag uccacagaaa acagcaagau gaugcuugag     1680

cuggaucccc cuuucggcga cucuuacauu gucaucgggg ugggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu cggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cuuaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuc auugggacac ugcugaugug gcucggacug     1980

aacacgaaga auggcagcau cucccuuaug ugucuggcuc ucgggggagu ucugaucuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2380
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short CDS opt

<400>  2380
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccggauu      420

gaaaauugga ucuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagugauu uaucuuguca ugauccugcu cauugcuccc      540

gccuacucca ucagaugcau uggggugagc aaucgggacu uuguugaggg aaugucuggc      600

gggacauggg uggaugucgu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggaucuc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcuc uugggggagu gcucaucuuc     2040

cugagcaccg cgguuucugc c                                               2061


<210>  2381
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short CDS opt

<400>  2381
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uucugcgcaa cccuggcuuu gcucucgccg cggccgcuau cgccuggcug      480

cuuggcucca gcacgucuca gaaagucauc uaucugguga ugauucuccu gaucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu ucguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuugaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuggugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accucgacaa gcagucugac acccaguaug uuugcaaacg gacacuggug      840

gaucgcggau ggggcaaugg cugugggcug uuuggaaagg gcagccucgu gaccugcgcc      900

aaauucgccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccuggaguac      960

agaaucaugc uuucugucca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccugg gaggcuuugg cagccucggg cuggacugug agccucgcac cggacuugau     1140

uucuccgacc uguauuaccu cacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuucacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccucgugga guucaaggau gcucacgcca aacggcagac ggucgugguu     1320

cugggaucuc aggaaggcgc ggugcauacc gcucuugccg gggcccugga ggcugaaaug     1380

gacggcgcca agggaagacu cagcuccggc caccugaaau gccgccuuaa gauggauaag     1440

cugcggcuca aaggggucag cuauucucug uguacagcug ccuuuaccuu cacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccucacacc ugugggaaga     1620

cugaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuugag     1680

cuggaucccc cuuuuggcga cucuuacauc gucaucggga uuggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu ucgaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu cggagauacc gccugggacu uuggcuccgu cgggggagcu     1860

cuuaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuucaa aagccuguuu     1920

ggaggcaugu ccugguucuc ucaaauccuc aucgggacac ugcugaugug gcucggacug     1980

aacacgaaga auggcagcau uucccuuaug ugucuggcuc ucgggggagu gcugaucuuu     2040

cugagcaccg cgguuucugc c                                               2061


<210>  2382
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short CDS opt

<400>  2382
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugucc accaccguca gcaacauggc cgaggugcgc      720

ucuuacugcu augaagcuac aaucuccgac auugccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauuguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagaucacac cgaauagccc cagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggcggucgug     1320

cucggaucuc aggagggcgc uguucauacc gcccuggccg gggcucuuga ggccgaaaug     1380

gacggcgcua agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagccg cguucaccuu uacaaagauc     1500

ccggccgaga cccugcaugg aacggucacc guggaaguuc aguacgcugg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcuguc gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg ccaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc guuauugggg ucggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggcgu uugaagccac cgugagaggg     1800

gcuaaacgga uggccgugcu gggagauacc gcuugggacu ucggcuccgu cgggggagcc     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau uucccugaug ugucuugcgc uugggggagu gcucaucuuc     2040

cugagcaccg ccguuucugc u                                               2061


<210>  2383
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short CDS opt

<400>  2383
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaacugc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccucacacc ugugggaaga     1620

cugaucaccg cuaaccccgu gauuacggag uccacagaaa acagcaagau gaugcuugag     1680

cuggaucccc cuuucggcga cucuuacauc guuaucgggg ucggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu cggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cuuaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuuuuc     1920

ggaggcaugu ccugguuuuc ucaaauucuc aucgggacac ugcugaugug gcucggacug     1980

aacacgaaga auggcagcau cucccuuaug ugucuggcuc ucgggggagu gcugauuuuc     2040

cugagcaccg cgguuucugc c                                               2061


<210>  2384
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short CDS opt

<400>  2384
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccaucgaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucauugggg ugggcgagaa aaagaucaca     1740

caccacuggc aucgcuccgg aagcaccauu ggcaaggccu uugaagcgac agugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggaccc uccugaugug gcugggacuc     1980

aacacaaaga auggcagcau uucccugaug ugucuugcuc uugggggagu ucucaucuuc     2040

cugagcacgg cggugucugc c                                               2061


<210>  2385
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short CDS opt

<400>  2385
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcguca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaaa     1440

cuucggcuga agggggugag cuauucucuc uguacagccg cuuucaccuu uacaaagauu     1500

ccggccgaga cccugcaugg aacgguuacc guggaagucc aguacgcggg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcugug gacaugcaga cccugacacc uguuggaaga     1620

cucaucaccg ccaaccccgu cauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gugaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggcuu uugaagccac cgucagaggg     1800

gcgaaacgga uggccgugcu gggagauacc gcuugggacu ucggcuccgu ugggggagcc     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg cugccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cuccuucaug ugucuggccc uugggggagu gcugauuuuu     2040

cucagcaccg cugucucugc g                                               2061


<210>  2386
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short CDS opt

<400>  2386
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaaaacu gagcuccggc caccucaagu gcagacugaa gauggauaaa     1440

cuucgccuga agggggucag cuauucucuc uguacagcug ccuucaccuu uacaaaaauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaagg uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggacgg     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaaaau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa gaagauuacc     1740

caccacuggc auagauccgg aagcacaauc ggcaaagccu uugaagcgac cgugcgcggg     1800

gccaagcgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaagg gauucaccag aucuucggcg ccgccuuuaa gagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaaa auggcagcau cucccugaug ugucuugcuc ucgggggagu ucugauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2387
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short CDS opt

<400>  2387
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaaggaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa caaaugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaauaccac auccaccugg gucguguaug ggacguguca ucacaagaaa      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaaacaccu gauccgggug      420

gaaaacugga uuuuucgcaa uccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaggucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaccgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaauauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa acagucugac acccaguaug uuugcaagcg gacacucgug      840

gaucgcggau ggggcaacgg cugugggcug uucggaaaag gcagccuggu gaccugcgcc      900

aaguuugccu gcuccaaaaa gaugacaggg aagucuauuc agccugaaaa ucucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa cgacaccggg     1020

caugaaacgg augagaaucg ggcuaaagug gagauuacac cgaacucucc cagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaau aacaagcacu ggcucgucca uaaagaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaau     1260

aacaaggagg cccuggugga auuuaaagau gcucacgcca agcggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca aaggaagacu gagcuccggc caccucaagu gccgccugaa gauggauaaa     1440

cuucggcuga agggggucag cuauucucuc uguacagcug ccuucaccuu uacaaaaauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaagg uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaucccgu uauuacggag uccacagaaa acagcaaaau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa gaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaagccu uugaagcgac cgugagaggg     1800

gccaagcgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaacucuc ugggcaaagg gauucaccag aucuucggcg ccgccuuuaa gagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aauacgaaaa acggcagcau cucccugaug ugucuugcuc ucgggggagu ucugauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2388
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short CDS opt

<400>  2388
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacagga aagagcauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cgggucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc cagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggag gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga agcugagaug     1380

gauggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggacaaa     1440

cuucggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaaa cccugcaugg aacggugacc guugaggugc aguacgccgg cacagauggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggaa uccacagaga acagcaagau gaugcuggag     1680

cuugacccgc cuuucggcga uucuuacauc gucaucgggg ugggcgaaaa gaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaggcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagacacc gccugggauu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugcuc ucgggggagu ucugauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2389
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short CDS opt

<400>  2389
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacagga aagagcauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cgggucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc cagagcggaa     1080

gccgcucuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcc ggcgccgaca cagggacccc gcauuggaac     1260

aauaaagagg cucuggugga auuuaaggau gcccacgcga aacggcagac agucgugguu     1320

cucggaucuc aggagggcgc ugugcauacg gcccuggccg gggcucuuga ggccgaaaug     1380

gacggcgcua agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguaccgccg cguucacauu uaccaagauu     1500

ccggccgaga cacugcaugg aaccgugacg guugaagugc aguacgcugg caccgacggg     1560

ccuugcaaag uccccgccca aauggcugug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacag ccaaccccgu uauuaccgag uccacggaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggcgu uugaagccac agugagaggg     1800

gcuaaacgga uggccguccu gggagauacc gcuugggacu ucggcuccgu cgggggagcc     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaacccuu auugggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugcgc uugggggagu ucucaucuuc     2040

cugagcaccg ccgugucugc u                                               2061


<210>  2390
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short CDS opt

<400>  2390
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cgcugacggg     1560

ccuugcaaag uccccgccca aauggcugug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacag ccaaccccgu uauuaccgag uccacggaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggcgu uugaagccac agugagaggg     1800

gcuaaacgga uggccguccu gggagauacc gcuugggacu ucggcuccgu cgggggagcc     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggaccc uccugaugug gcugggacuc     1980

aacacaaaga auggcagcau cucccugaug ugucuugcgc uugggggagu ucucauuuuc     2040

cugagcacgg ccgugucugc u                                               2061


<210>  2391
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short CDS opt

<400>  2391
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugccccaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcucug      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuugaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagcccacag uggauauuga gcuggugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug uccuacgcaa      780

ggggaggcuu accucgacaa gcagucugac acccaguaug uuugcaaacg gacacuggug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccucgu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agcccgaaaa ccuggaguac      960

agaaucaugc uuucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac cuaauucucc gagagcggaa     1080

gccacccugg gaggcuucgg cagccucggg cuggacugug agccccgcac cggacuugau     1140

uuuuccgacc uguauuaccu cacaaugaac aauaaacacu ggcuggucca uaaggaaugg     1200

uuccacgaua ucccucugcc cuggcacgcu ggcgccgaca ccgggacacc ucauuggaac     1260

aauaaagagg cccucgugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cugggaucuc aggagggcgc ggugcauacc gcucuugccg gggcccugga ggcugaaaug     1380

gacggcgcca agggaagacu cagcuccggc caccugaaau gccgccuuaa gauggauaag     1440

cugcggcuca aaggggucag cuauucucug uguacagcug ccuucaccuu uacaaagauu     1500

cccgcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccgugcaaag ucccugcuca aauggccgug gacaugcaga cccucacacc cgugggaaga     1620

cugaucaccg cuaacccugu uauuacggag uccacagaaa acagcaagau gaugcuugag     1680

cuggauccuc ccuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu cggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cuuaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuc aucgggacac ugcugaugug gcucggacug     1980

aacacgaaga auggcagcau cucccuuaug ugucuggcuc ucgggggagu ucugauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2392
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short CDS opt

<400>  2392
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac augguuagcg auuccagaug ucccacgcaa      780

ggggaggccu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguc      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcu      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucuguuca cggaucccaa cacagcggca ugaucgugaa ugacaccggg     1020

caugaaacgg augagaaccg ggccaagguc gagauuacac ccaauucucc gagagcugaa     1080

gcgacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcc ggcgcugaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcccacgcua aacggcagac ggugguugug     1320

cucggaucuc aggagggcgc cguccauacc gcgcuggcug gggcccuuga ggccgaaaug     1380

gacggcgcua agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggugag cuauucucuc uguacagccg cuuucaccuu uacaaagauu     1500

ccggccgaga cccugcaugg aacgguuacc guggaagucc aguacgcggg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcugug gacaugcaga cccugacacc uguuggaaga     1620

cucaucaccg ccaaccccgu cauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gugaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggcuu uugaagccac cgucagaggg     1800

gcgaaacgga uggccgugcu gggagauacc gcuugggacu ucggcuccgu ugggggagcc     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg cugccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugccc uugggggagu gcucauuuuc     2040

cugagcaccg cugucucugc g                                               2061


<210>  2393
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short CDS opt

<400>  2393
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggauuagc aaucgggacu uuguugaggg aaugucuggc      600

gggacauggg uggaugucgu gcuggaacau ggaggcugug ugaccgucau ggcgcaggac      660

aagccuacag uugauaucga gcuugugacc accacagugu ccaacauggc cgagguccgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugauugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaagguu gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cauucugggg cucgacugug agccucgcac cggacuggau     1140

uuuagcgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac gguggucgug     1320

cucggauccc aggagggcgc gguucauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gucuagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aagggguguc cuauagccuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggucacc guggaaguuc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu gaucacggag ucuacagaaa acuccaagau gaugcuggag     1680

cuugaucccc cuuucggcga cagcuacauu guuaucgggg ucggcgagaa aaagauuacc     1740

caccacuggc aucgcucugg auccacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcagcgu cgggggagcu     1860

cucaauuccc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aucucuguuc     1920

ggaggcauga gcugguuuuc ccaaauccuu auugggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcucuau cagccugaug ugucuugcuc uugggggagu gcucauuuuc     2040

cuguccaccg cgguuagcgc c                                               2061


<210>  2394
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short CDS opt

<400>  2394
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacacaugu      180

gaugccacca ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacacaac cuccacgugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acacuuccca gccauuccau ccggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauucgggug      420

gaaaauugga ucuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauu uaucuuguga ugauccugcu cauugcuccc      540

gccuacucca ucagaugcau ugggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauaucga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugauuguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu uaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucauugggg ugggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu aucgggacac uccugaugug gcugggacuc     1980

aacgcuaaga auggcagcau uucccugaug ugucuugcgc uugggggagu ucucaucuuc     2040

cugagcacgg ccgugucugc u                                               2061


<210>  2395
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short CDS opt

<400>  2395
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcuc aaagaucucg gcgcgccguu acccuuccca gccauuccac aagaaaacug      360

cagacccggu cucagacaug gcucgagagc cgcgaguaca ccaagcaccu gaucagagug      420

gaaaauugga uuuuucggaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uucgcugcau cgggguuagc aauagagacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaagac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgg      720

agcuacugcu augaagcuag caucuccgac auggccagcg auucccgcug ucccacgcag      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaaag aacacucgug      840

gaucggggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc aaccugaaaa ccucgaguac      960

cgcaucaugc ugucugugca cggaucccag cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaacag agcuaaggug gagauuacac ccaauucucc gcgggcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccuagaac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacgccagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggacggcu gagcuccggc caccucaaau gcagacugaa gauggauaag     1440

cugcgccuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aauacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca gauggccgug gacaugcaga cccugacacc ugugggacgg     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc auagauccgg aagcacaauc ggcaaggccu uugaagcgac cgugcgcggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauucaccaa aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucagauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2396
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short CDS opt

<400>  2396
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caagcucgcu cucagaccug gcucgagagc agagaguaca caaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcccuggcgg ccgcugccau cgcuuggcuc      480

cugggcucca gcaccucuca gaaagucauc uaucuuguga ugauucugcu caucgccccc      540

gcguacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacguggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcccaggac      660

aagccuaccg uggauauuga gcuugugaca accaccgucu ccaacauggc ugaggugcgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacacaa      780

ggggaggccu accuggacaa gcagucugac acgcaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu cacaugcgcc      900

aaauuugcuu gcuccaagaa aaugaccgga aagagcauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cgggucccaa cacagcggca ugaucguuaa ugacacaggg     1020

caugaaaccg augagaaccg ggcgaaggug gagauuacgc ccaauucucc cagagccgaa     1080

gcuacacuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcc ggcgccgaca cagggacccc gcauuggaac     1260

aauaaagagg cucuggugga auuuaaggau gcccacgcga aacggcagac agucgugguu     1320

cucggaucuc aggagggcgc ugugcauacg gcccuggccg gggcucuuga ggccgaaaug     1380

gacggcgcua agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguaccgccg cguucacauu uaccaagauu     1500

ccggccgaga cacugcaugg aaccgugacg guugaagugc aguacgcugg caccgacggg     1560

ccuugcaaag uccccgccca aauggcugug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacag ccaaccccgu uauuaccgag uccacggaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggcgu uugaagccac agugagaggg     1800

gcuaaacgga uggccguccu gggagauacc gcuugggacu ucggcuccgu ugggggagcc     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggaccc uccugaugug gcugggacuc     1980

aacacaaaga auggcagcau cucccugaug ugucuugcgc uugggggagu gcucauuuuc     2040

cugagcacgg ccgugucugc u                                               2061


<210>  2397
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short CDS opt

<400>  2397
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcgaagc cauuagcuuc      120

cccacaaccc uggggaugaa caagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggagccg cagagccguu acccuucccu cccauucuac acggaaacug      360

caaacccgca gccagacaug gcucgagucc agagaguaca ccaagcaccu gauccgggug      420

gaaaacugga uuuuucgcaa uccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcagcu cuacguccca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacagca uuagaugcau cgggguuucu aaccgggacu uuguggaggg aauguccggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacaguca gcaauauggc cgaggugcgc      720

ucuuacugcu augaagccuc caucagcgac auggcuuccg auucuagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagagcgac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcc      900

aaauuugccu gcagcaagaa aaugacaggg aagucuauuc agccugaaaa ucucgaguac      960

agaaucaugc uguccgucca cggaagccaa cacucuggca ugaucguuaa cgacaccggg     1020

caugaaacgg augagaaucg ggcuaaggug gagauuacac cgaacagccc cagagcggaa     1080

gccacccuug gaggcuucgg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uuuucugacc uuuauuaccu gacaaugaau aacaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaau     1260

aacaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaagcc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaaa     1440

cuucggcuga aaggggucuc uuauucccuc uguacagcug ccuucaccuu uagcaagauu     1500

ccggcggaga cacugcaugg aaccgugacg gaagagguuc aguacgccgg caccgacggg     1560

ccuugcaaag ugcccgcuca aauggccguc gacaugcaga cacugacccc ugugggaaga     1620

cucaucacag cuaaucccgu gauuaccgaa ucuacggaga acuccaagau gaugcuggag     1680

cuugaucccc cuuucggcga cagcuacauc guuaucgggg ucggcgaaaa gaagauuaca     1740

caccacuggc aucgcuccgg aucuaccauc ggcaaggccu uugaggcgac agugagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcagcgu cgggggagcu     1860

cucaacuccc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aucucuguuc     1920

ggaggcauga gcugguuuuc ccaaauucuu aucgggaccc uccugaugug gcugggacuc     1980

aauacaaaga acggcagcau cucucugaug ugucuugcuc ucgggggagu gcugauuuuc     2040

cuguccacgg cgguuagcgc c                                               2061


<210>  2398
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short CDS opt

<400>  2398
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcgaagc cauuagcuuc      120

cccacaaccc uggggaugaa caagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggagccg cagagccguu acccuucccu cccauucuac acggaaacug      360

caaacccgca gccagacaug gcucgagucc agagaguaca ccaagcaccu gauccgggug      420

gaaaacugga uuuuucgcaa uccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcagcu cuacguccca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacagca uuagaugcau cgggguuucu aaccgggacu uuguggaggg aauguccggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacaguca gcaauauggc cgaggugcgc      720

ucuuacugcu augaagccuc caucagcgac auggcuuccg auucuagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagagcgac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcc      900

aaauuugccu gcagcaagaa aaugacaggg aagucuauuc agccugaaaa ucucgaguac      960

agaaucaugc uguccgucca cggaagccaa cacucuggca ugaucguuaa cgacaccggg     1020

caugaaacgg augagaaucg ggcuaaggug gagauuacac cgaacagccc cagagcggaa     1080

gccacccuug gaggcuucgg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uuuucugacc uuuauuaccu gacaaugaau aacaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacacc ggcgcugaca cagggacgcc ccauuggaau     1260

aacaaagagg cccuggugga auuuaaggau gcccacgcua aacggcagac cgucgugguu     1320

cucggaagcc aggagggcgc cgugcauaca gcgcuggcug gggcccuuga ggccgaaaug     1380

gacggcgcua agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaaa     1440

cuucggcuga agggggucuc uuauucccuc uguaccgccg cuuucacauu uaccaagauu     1500

ccggccgaga cgcugcaugg aaccgugaca guugaagugc aguacgcggg caccgacggg     1560

ccuugcaaag uccccgccca aauggcugug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacgg ccaaucccgu uauuacagag ggcaccgaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucgggga cucuuacauc gucaucggcg ugggagagaa aaagauuaca     1740

caccacuggc aucgcuccgg cagcaccauc gggaaggcuu uugaagccac cgugcgggga     1800

gcgaaaagaa uggccguccu gggcgauaca gcuugggacu ucggguccgu gggaggcguu     1860

cucaacucuc uggggaaggg cauucaccag aucuucggag ccgcuuuuaa aagccuguuc     1920

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacgc uccugaugug gcugggccuc     1980

aauaccaaga acgggagcau cucccugaug ugucuugccc ugggaggcgu gcucauuuuc     2040

cugagcacag ccgucucugc u                                               2061


<210>  2399
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short CDS opt

<400>  2399
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcgaagc cauuagcuuc      120

cccacaaccc uggggaugaa caagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggagccg cagagccguu acccuucccu cccauucuac acggaaacug      360

caaacccgca gccagacaug gcucgagucc agagaguaca ccaagcaccu gauccgggug      420

gaaaacugga uuuuucgcaa uccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcagcu cuacguccca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacagca uuagaugcau cgggguuucu aaccgggacu uuguggaggg aauguccggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacaguca gcaauauggc cgaggugcgc      720

ucuuacugcu augaagccuc caucagcgac auggcuuccg auucuagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagagcgac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcc      900

aaauuugccu gcagcaagaa aaugacaggg aagucuauuc agccugaaaa ucucgaguac      960

agaaucaugc uguccgugca cggaagccaa cacucuggca ugaucguuaa cgacaccggg     1020

caugaaacgg augagaaucg ggcuaaggug gagauuacac cgaacagccc cagagcggaa     1080

gccacccuug gaggcuucgg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uuuucugacc uuuauuaccu gacaaugaau aacaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaau     1260

aacaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaagcc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucuc uuauucccuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaucccgu uauuacggag agcacagaaa acucuaagau gaugcuggag     1680

cuugaucccc cuuucggcga cuccuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcagcgg auccacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcucugu cgggggagcu     1860

cucaacagcc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aucccuguuc     1920

ggaggcaugu cuugguuuag ccaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aauacgaaga acggcuccau cagccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugucuaccg cgguguccgc c                                               2061


<210>  2400
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short CDS opt

<400>  2400
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcuguuga ggugaccaga       60

cggggaaacg ccuacuauau guaccuugac cgcuccgaug cgggcgaagc uauuucuuuc      120

cccacaacca uggggaugaa uaagugcuau auccagauua uggaccuggg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cucgacgaag gcguggagcc ggaugacguc      240

gauuguuggu gcaacaccac auccaccugg gugguuuaug ggacguguca ucacaaaaag      300

ggagaagcca gacggucucg cagagcugug acccugccca gccauuccac acggaaacuu      360

caaacccgcu cucagacaug gcuggagagc agagaguaca ccaagcaccu cauccggguc      420

gaaaauugga uuuuucgcaa cccuggcuuc gcccuggcug ccgcggccau cgcuuggcug      480

cucggcucca gcacgucuca gaaagugauc uaucugguua ugauucuucu gaucgccccc      540

gcuuacucca uuagaugcau cggggugagc aaucgggacu uugucgaggg aaugucuggc      600

gggacauggg uggauguggu ccucgaacau ggaggcugug uuaccgugau ggcccaggac      660

aagccuacag uggauauuga gcuggucacc accacagugu ccaacauggc ggagguucgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaggccu accuugacaa gcagucugac acccaguaug ugugcaaacg gacacugguc      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcu      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccuggaguac      960

agaaucaugc ucucuguuca cggaucccaa cacagcggca ugaucgugaa ugacaccggg     1020

caugaaacgg augagaaccg ggccaagguc gagauuacac cgaauagccc cagagcugaa     1080

gcgacccugg gaggcuucgg cagccuuggg cuggacugug agccucgcac cggacucgau     1140

uuuuccgacc uguauuaccu uacaaugaac aauaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaua ucccccuccc uuggcacgcc ggcgcugaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggucga auuuaaggau gcccacgcua aacggcagac ggugguugug     1320

cugggaucuc aggagggcgc cguccauacc gcgcucgcug gggcccugga ggccgaaaug     1380

gacggcgcua agggaagacu uagcuccggc caccugaaau gccgccucaa gauggauaag     1440

cugcggcuua aaggggugag cuauucucug uguacagccg cuuucaccuu uacaaagauu     1500

ccggccgaga cccuccaugg aacgguuacc guggaagucc aguacgcggg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcugug gacaugcaga cccugacacc uguuggaaga     1620

cugaucaccg ccaaccccgu cauuacggag uccacagaaa acagcaagau gaugcucgag     1680

cuggaucccc cuuucggcga cucuuacauc gugaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggcuu uugaagccac cgucagaggg     1800

gcgaaacgga uggccgugcu uggagauacc gcuugggacu ucggcuccgu ugggggagcc     1860

cucaauucuc ucggcaaggg gauucaccag aucuucggcg cugccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucug aucgggacac uucucgugug gcugggacug     1980

aacacgaaga auggcagcau cucccucaug ugucuggccc uugggggagu ccugauuuuc     2040

cucagcaccg cugugucugc g                                               2061


<210>  2401
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short CDS opt

<400>  2401
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcgaagc cauuagcuuc      120

cccacaaccc uggggaugaa caagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggagccg cagagccguu acccuucccu cccauucuac acggaaacug      360

caaacccgca gccagacaug gcucgagucc agagaguaca ccaagcaccu gauccgggug      420

gaaaacugga uuuuucgcaa uccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcagcu cuacguccca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacagca uuagaugcau cgggguuucu aaccgggacu uuguggaggg aauguccggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacaguca gcaauauggc cgaggugcgc      720

ucuuacugcu augaagccuc caucagcgac auggcuuccg auucuagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagagcgac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcc      900

aaauuugccu gcagcaagaa aaugacaggg aagucuauuc agccugaaaa ucucgaguac      960

agaaucaugc uguccgugca cggaagccaa cacucuggca ugaucguuaa cgacaccggg     1020

caugaaacgg augagaaucg ggcuaaggug gagauuacac cgaacagccc cagagcggaa     1080

gccacccuug gaggcuucgg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uuuucugacc uuuauuaccu gacaaugaau aacaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaau     1260

aacaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaagcc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucuc uuauucccuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaucccgu uauuacggag agcacagaaa acucuaagau gaugcuggag     1680

cuugaucccc cuuucggcga cuccuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcagcgg auccacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcucugu cgggggagcu     1860

cucaacagcc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aucccuguuc     1920

ggaggcaugu cuugguuuag ccaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aauacgaaga acggcuccau cagccugguu ugucuugcuc uugggggagu gcucauuuuc     2040

cugucuaccg cggucuccgc c                                               2061


<210>  2402
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short CDS opt

<400>  2402
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcgaagc cauuagcuuc      120

cccacaaccc uggggaugaa caagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggagccg cagagccguu acccuucccu cccauucuac acggaaacug      360

caaacccgca gccagacaug gcucgagucc agagaguaca ccaagcaccu gauccgggug      420

gaaaacugga uuuuucgcaa uccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcagcu cuacguccca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacagca uuagaugcau cgggguuaac aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugguu ugucuugcuc uugggggagu gcucauuuuc     2040

cugagcaccg cggucucugc c                                               2061


<210>  2403
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short CDS opt

<400>  2403
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcuguuga ggugaccaga       60

cggggauccg ccuacuauau guaccuugac cgcucugaug cgggcgaagc uauuagcuuc      120

cccacaaccc uggggaugaa caagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga gugcccuaug cuggacgaag gcguggagcc ggaugacguc      240

gauuguuggu gcaauaccac aucuaccugg gugguuuaug ggacguguca ucacaaaaag      300

ggagaagcca gacggagccg cagagcugug acccuucccu cccauucuac acggaaacug      360

caaacccgca gccagacaug gcucgagucc agagaguaca ccaagcaccu gauccggguc      420

gaaaacugga uuuuucgcaa uccuggcuuc gcccuggcug ccgcggccau cgcuuggcuc      480

cugggcagcu cuacguccca gaaagugauc uaucuuguua ugauucugcu caucgccccc      540

gcuuacagca uuagaugcau cggggugucu aaccgggacu uugucgaggg aauguccggc      600

gggacauggg uggauguggu ccuggaacau ggaggcugug uuaccgugau ggcccaggac      660

aagccugcgg uggauauuga gcuugucaca accaccguga gcaauauggc cgagguucgc      720

ucuuacugcu augaagccuc caucagcgac auggcuuccg auucuagaug ucccacacaa      780

ggggaggcuu accuggacaa gcagagcgac acgcaguaug ugugcaaacg gacccucguc      840

gaucgcggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gacaugcgcc      900

aaauuugccu gcagcaagaa aaugaccggg aagucuauuc agccugaaaa ucucgaguac      960

agaaucaugc uguccguuca cggaagccaa cacucuggca ugaucgugaa cgacacaggg     1020

caugaaaccg augagaaucg ggcuaagguc gagauuacgc cgaacagccc cagagcggaa     1080

gccacacuug gaggcuucgg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uuuucugacc uuuauuaccu gaccaugaau aacaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca cagggacacc ccauuggaau     1260

aacaaagagg cccuggucga auuuaaggau gcucacgcca aacggcagac cgugguugug     1320

cucggaagcc aggagggcgc gguccauacg gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aagggguguc uuauucccuc uguaccgcug ccuucacauu uaccaagauu     1500

ccggcggaga cacugcaugg aaccguuacg guggaagucc aguacgccgg caccgacggg     1560

ccuugcaaag ugcccgcuca aauggccgug gacaugcaga cacugacccc uguuggaaga     1620

cucaucacag cuaaucccgu cauuaccgag agcacggaaa acucuaagau gaugcuggag     1680

cuugaucccc cuuucggcga cuccuacauc gugaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcagcgg auccaccauc ggcaaggccu uugaagcgac agucagaggg     1800

gccaaacgga uggcugugcu gggagauacc gccugggacu ucggcucugu ugggggagcu     1860

cucaacagcc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aucccuguuc     1920

ggaggcaugu cuugguuuag ccaaauucuu aucgggaccc uccuggugug gcugggacuc     1980

aauacaaaga acggcuccau cagccugacg ugucuugcuc uugggggagu ccucauuuuc     2040

cugucuaccg cgguguccgc c                                               2061


<210>  2404
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short CDS opt

<400>  2404
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcuguuga ggugaccaga       60

cggggauccg ccuacuauau guaccuugac cgcucugaug cgggcgaagc uauuagcuuc      120

cccacaaccc uggggaugaa caagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga gugcccuaug cuggacgaag gcguggagcc ggaugacguc      240

gauuguuggu gcaauaccac aucuaccugg gugguuuaug ggacguguca ucacaaaaag      300

ggagaagcca gacggagccg cagagcugug acccuucccu cccauucuac acggaaacug      360

caaacccgca gccagacaug gcucgagucc agagaguaca ccaagcaccu gauccggguc      420

gaaaacugga uuuuucgcaa uccuggcuuc gcccuggcug ccgcggugau cgccuggcuc      480

cugggcagcu cuacguccca gaaaguuauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacagca uuagaugcau cggggucucu aaccgggacu uuguggaggg aauguccggc      600

gggacauggg uggaugucgu ucuggaacau ggaggcugug ugaccgugau ggcucaggac      660

aagccuacag ucgauauuga gcuugugacc accacaguua gcaauauggc cgaggugcgc      720

ucuuacugcu augaagcguc caucagcgac auggccuccg auucuagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagagcgac acccaguaug ucugcaaacg gacacucgug      840

gaucgcggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu uaccugcgcc      900

aaauuugcuu gcagcaagaa aaugacaggg aagucuauuc agccugaaaa ucucgaguac      960

agaaucaugc uguccgugca cggaagccaa cacucuggca ugaucgucaa cgacaccggg     1020

caugaaacgg augagaaucg ggccaaggug gagauuacac cgaacagccc cagagccgaa     1080

gcuacccuug gaggcuucgg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uuuucugacc uuuauuaccu gacaaugaau aacaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcg ggcgccgaca ccgggacacc ccauuggaau     1260

aacaaagagg cucuggugga auuuaaggau gcccacgcca aacggcagac gguugugguc     1320

cucggaagcc aggagggcgc ugugcauacc gcccuggcgg gggcucuuga ggccgaaaug     1380

gacggcgcca agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aagggguuuc uuauucccuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcugaga cccugcaugg aacggugacc gucgaagugc aguacgccgg cacagacggg     1560

ccuugcaaag ugcccgcgca aauggccguu gacaugcaga cccugacacc ugucggaaga     1620

cucaucaccg cuaaucccgu gauuacggag agcacagaaa acucuaagau gaugcuggag     1680

cuugaucccc cuuucggcga cuccuacauc gugaucgggg ucggcgagaa aaagauuacc     1740

caccacuggc aucgcagcgg auccacaauc ggcaaggccu uugaagcuac cgugagaggg     1800

gccaaacgga uggcgguucu gggagauacc gccugggacu ucggcucugu cgggggagcu     1860

cucaacagcc ugggcaaggg gauucaccag aucuucggcg ccgcuuuuaa aucccuguuc     1920

ggaggcaugu cuugguuuag ccaaauucuu aucgggacac uccuggugug gcugggacuc     1980

aauacgaaga acggcuccau cagccugacc ugucuugccc uugggggagu gcucauuuuc     2040

cugucuacag ccguuuccgc u                                               2061


<210>  2405
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short CDS opt

<400>  2405
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcuguuga ggugaccaga       60

cggggauccg ccuacuauau guaccuugac cgcucugaug cgggcgaagc uauuagcuuc      120

cccacaaccc ugggggugaa caagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga gugcccuaug cuggacgaag gcgucgagcc ggaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guuguguaug ggacguguca ucacaaaaag      300

ggagaagcca gacggagccg cagagcuguc acccuucccu cccauucuac acggaaacug      360

caaacccgca gccagacaug gcucgagucc agagaguaca ccaagcaccu gauccgggug      420

gaaaacugga uuuuucgcaa uccuggcuuc gcccuggcug ccgcggccau cgcuuggcuc      480

cugggcagcu cuacguccca gaaaguuauc uaucuuguga ugauucugcu caucgccccc      540

gcuuacagca uuagaugcau cggggucucu aaccgggacu uuguggaggg aauguccggc      600

gggacauggg uggaugucgu ucuggaacau ggaggcugug ugaccgugau ggcccaggac      660

aagccuacag ucgauauuga gcuugugacc accacaguua gcaauauggc ggaggugcgc      720

ucuuacugcu augaagccuc caucagcgac auggcuuccg auucuagaug ucccacgcaa      780

ggggaggccu accuggacaa gcagagcgac acccaguaug ucugcaaacg gacacucgug      840

gaucgcggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu uaccugcgcu      900

aaauuugccu gcagcaagaa aaugacaggg aagucuauuc agccugaaaa ucucgaguac      960

agaaucaugc uguccgugca cggaagccaa cacucuggca ugaucgucaa cgacaccggg     1020

caugaaacgg augagaaucg ggccaaggug gagauuacac cgaacagccc cagagcugaa     1080

gcgacccuug gaggcuucgg cucccugggg cucgacugug agccucgcac cggacuggau     1140

uuuucugacc uuuauuaccu gacaaugaau aacaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcc ggcgcugaca ccgggacacc ccauuggaau     1260

aacaaagagg cccuggugga auuuaaggau gcccacgcua aacggcagac gguugugguc     1320

cucggaagcc aggagggcgc cgugcauacc gcgcuggcug gggcccuuga ggccgaaaug     1380

gacggcgcua agggaagacu guccagcggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aagggguuuc uuauucccuc uguacagccg cuuucaccuu uacaaagauu     1500

ccggccgaga cccugcaugg aacggugacc gucgaagugc aguacgcggg cacagacggg     1560

ccuugcaaag ugcccgccca aauggcuguu gacaugcaga cccugacacc ugucggaaga     1620

cucaucaccg ccaaucccgu gauuacggag agcacagaaa acucuaagau gaugcuggag     1680

cuugaucccc cuuucggcga cuccuacauc gugaucgggg ucggcgauaa aaagauuacc     1740

caccacuggu aucgcagcgg auccacaauc ggcaaggcuu uugaggccac cgugagaggg     1800

gcgaaacgga uggccguucu gggagacacc gcuugggauu ucggcucugu cgggggagcc     1860

cucaacagcc ugggcaaggg gauucaucag aucuucggcg cugccuuuaa aucccuguuc     1920

ggaggcaugu cuugguuuag ccaaauucuu aucgggacac uccuggugug gcugggacuc     1980

aauacgaaga acggcuccau cagccugacc ugucuugccc uugggggagu gcucauuuuc     2040

cugucuacag cuguuuccgc g                                               2061


<210>  2406
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short CDS opt

<400>  2406
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcuguuga ggugaccaga       60

cggggauccg ccuacuauau guaccuugac cgcucugaug cgggcaaggc uauuagcuuc      120

cccacaaccc ugggggugaa caaaugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa uguccuacga augcccuaug cuggacgagg gcgucgaacc ggaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guuguguaug ggacguguca ucacaagaaa      300

ggagaggcca gacggagccg cagagcuguc acccuucccu cccauucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaca ccaaacaccu gauccgggug      420

gagaacugga uuuuucgcaa uccuggcuuc gcccuggcug ccgcggccau cgcuuggcuc      480

cugggcagcu cuacguccca gaagguuauc uaucuuguga ugauucugcu caucgccccc      540

gcuuacagca uuagaugcau cggggucucu aaccgggacu uuguggaagg aauguccggc      600

gggacauggg uggaugucgu ucuggagcau ggaggcugug ugaccgugau ggcccaggac      660

aagccuacag ucgauauuga acuugugacc accacaguua gcaauauggc ggaggugcgc      720

ucuuacugcu augaggccuc caucagcgac auggcuuccg auucuagaug ucccacgcaa      780

ggggaagccu accuggacaa acagagcgac acccaguaug ucugcaagcg gacacucgug      840

gaucgcggau ggggcaacgg cugugggcug uucggaaaag gcucccuggu uaccugcgcu      900

aaguuugccu gcagcaaaaa gaugacaggg aagucuauuc agccugagaa ucucgaauac      960

agaaucaugc uguccgugca cggaagccaa cacucuggca ugaucgucaa cgacauuggg     1020

caugagaccg augaaaaucg ggccaaagug gagaucacgc cgaacagccc cagagcugag     1080

gcgacacuug gaggcuucgg cucccugggg cucgacugug aaccucgcac cggacuggau     1140

uuuucugacc uuuauuaccu gaccaugaau aacaagcacu ggcucgucca uaaagagugg     1200

uuccacgaua uuccccugcc uuggcacgcc ggcgcugaca cagggacacc ccauuggaau     1260

aacaaggaag cccuggugga guuuaaagau gcccacgcua agcggcagac aguugugguc     1320

cucggaagcc aggaaggcgc cgugcauacg gcgcuggcug gggcccuuga ggccgagaug     1380

gacggcgcua aaggaagacu guccagcggc caccucaagu gccgccugaa gauggauaaa     1440

cuucggcuga aggggguuuc uuauucccuc uguaccgccg cuuucacauu uaccaaaauc     1500

ccggccgaaa cacugcaugg aaccgugacg gucgaggugc aguacgcggg caccgacggg     1560

ccuugcaagg ugcccgccca aauggcuguu gacaugcaga cacugacccc ugucggaaga     1620

cucauuacag ccaaucccgu gaucaccgaa agcacggaga acucuaaaau gaugcuggaa     1680

cuugaucccc cuuucggcga cuccuacauc gugauugggg ucggcgauaa gaagaucaca     1740

caccacuggc aucgcagcgg auccaccauu ggcaaggcuu uugaggccac agugagaggg     1800

gcgaaacgga uggccguucu gggagacacc gcuugggauu ucggcucugu cgggggagcc     1860

cucaacagcc ugggcaaagg gauccaccag auuuucggcg cugccuuuaa gucccuguuc     1920

ggaggcaugu cuugguuuag ccaaauccuu aucgggaccc uccuggugug gcugggacuc     1980

aauaccaaaa acggcuccau uagccugacg ugucuugccc ucgggggagu gcugaucuuc     2040

cugucuaccg cuguuuccgc g                                               2061


<210>  2407
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short CDS opt

<400>  2407
accagcaucg gcauuguggg gcugcuccug acaacggcca uggcugccga gaucaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc cauuagcuuc      120

gccacaaccc ugggggucaa caaaugccac guucagauca uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguggaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcuguu acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gauuaaggug      420

gagaacugga ucuuucggaa ucccggcuuc acgcuggucg ccguggcgau cgccuggcuc      480

cugggcagcu cuacauccca gaagguuauu uaccuuguga ugauccugcu cauugcuccu      540

gccuauagca uccgcugcau uggggucucu aacagagacu uuguggaagg aauguccggc      600

gggaccuggg uggaugucgu ucuggagcac ggaggcugug ugacagugau ggcucaggac      660

aaacccaccg ucgauaucga acuugugacc acaacgguua gcaauauggc cgaggugcgg      720

ucuuacugcu acgaggcguc cauuagcgac auggccuccg auucucgcug uccuacccaa      780

ggggaggcuu aucuggacaa gcagagcgac acacaguacg ucugcaaaag aacccucgug      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu uacaugcgcc      900

aaauuuaccu gcagcaagaa gaugacgggg aaaucuaucc agcccgaaaa ucucgaauau      960

cgcaucaugc uguccgugca cggaagccaa cauucuggca ugauugucaa cgacacaggg     1020

cacgagaccg augaaaauag agcuaaggug gaggucaccc cuaacagccc gcgggccgag     1080

gccacacuug gaggcuucgg cucccugggg cucgacugug aacccagaac cggacuggau     1140

uuuucugacc uuuacuaucu gacaaugaau aacaaacauu ggcucgugca caaggagugg     1200

uuccacgaua ucccucugcc cuggcaugcu ggcgcggaca cggggacccc ucacuggaau     1260

aacaaagaag cccugguuga guuuaaggau gcucaugcca aacgccagac aguggucgug     1320

cucggaagcc aggaaggcgc cguucacacc gcucuggccg gggcgcuuga ggcugagaug     1380

gacggcgcca agggacggcu guucuccggc caucucaagu gcagacugaa aauggauaaa     1440

cuucgccuga agggggugag cuacucucuc uguacagccg cuuuuaccuu cacgaagguc     1500

cccgccgaaa cccugcacgg aacagugacc guggagguuc aguaugcugg cacagacggg     1560

ccgugcaaag ucccugccca aauggcggug gacaugcaga cccugacgcc cgugggacgg     1620

cucauuacag ccaauccugu caucaccgaa uccacagaga acagcaagau gaugcuggaa     1680

cuugauccuc ccuuuggcga cucuuacauu gugaucgggg uuggcgauaa gaaaaucacc     1740

caccauuggc acagauccgg aagcaccauu ggcaaggcuu ucgaggccac agugcgcggg     1800

gcuaaacgga uggccguccu gggagacacg gcgugggauu ucggcuccgu ugggggagug     1860

uuuaacucuc ucggcaaggg gauccaucag auuuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug auugggaccc uucucgugug gcugggacug     1980

aauacaaaga acggcagcau cucccucacc ugucuggccc uugggggagu caugaucuuc     2040

cugagcacag cugugucugc c                                               2061


<210>  2408
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short CDS opt

<400>  2408
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaacc ugggggugaa uaaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacca uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaacacaac cucuacaugg guggucuacg ggaccuguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acgcuuccgu cccacucuac ccggaagcug      360

caaacacgca gccagaccug gcucgaaucc agagaguaua caaaacaucu gaucaagguu      420

gagaauugga ucuuucggaa ccccggcuuc gcccuggcgg ccguggcuau ugccuggcuc      480

cugggcagcu cuaccuccca gaaggucauc uaccuuguga ugauucugcu caucgcuccu      540

gccuauagca uucgcugcau cgggguuucu aauagagacu uuguggaagg aauguccggc      600

gggacguggg ucgauguggu gcuggagcac ggaggcugug ucacaguuau ggcgcaggac      660

aaacccaccg uggauauuga acuugugaca accaccguca gcaacauggc cgaggugcgg      720

ucuuacugcu acgaggcuuc caucagcgac auggccuccg auucucgcug uccuacacaa      780

ggggaggcuu aucuggacaa gcagagcgac acgcaguacg uuugcaaaag aacccucgug      840

gaucggggau ggggcaaugg cugugggcug uucggaaagg gcucccuggu cacaugcgcc      900

aaauuuaccu gcagcaagaa gaugacaggg aaaucuaucc agcccgaaaa ccucgaauau      960

cgcauuaugc uguccgugca cggaagccaa cauucuggca ugaucguuaa ugacauuggg     1020

cacgagaccg augaaaacag agccaaggug gaggucacgc cuaauagccc gcgggcugag     1080

gcgacacuug gaggcuucgg cucccugggg cucgacugug aacccagaac cggacuggau     1140

uuuucugacc uuuacuaucu gaccaugaac aauaaacauu ggcucgugca caaggagugg     1200

uuccacgaua ucccucugcc cuggcaugcc ggcgcugaca cagggacccc ucacuggaac     1260

aauaaagaag cccuggucga guuuaaggau gcccaugcua aacgccagac agugguugug     1320

cucggaagcc aggaaggcgc cguccacacg gcgcuggcug gggcccuuga ggccgagaug     1380

gacggcgcua agggacggcu guucuccggc caucucaagu gcagacugaa aauggauaaa     1440

cuucgccuga agggggugag cuacucucuc uguaccgccg cuuuuacauu caccaagguu     1500

cccgccgaaa cacugcacgg aaccgugacg gucgaggugc aguaugcggg caccgacggg     1560

ccgugcaaag ugccugccca aauggcuguu gacaugcaga cacugacccc cgucggacgg     1620

cucauuacag ccaacccugu gaucaccgaa uccacggaga acagcaagau gaugcuggaa     1680

cuugauccuc ccuuuggcga cucuuacauc gugauugggg ucggcgauaa gaaaaucaca     1740

caccauuggc acagauccgg aagcaccauu ggcaaggcuu ucgaggccac agugcgcggg     1800

gcgaaacgga uggccguucu gggagacacc gcuugggauu ucggcuccgu cgggggagug     1860

uuuaauucuc ucggcaaggg ggugcaucag aucuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucug aucgggaccc uucucguuug gcugggacug     1980

aacacaaaga auggcagcau cucccucacg ugucuggccc uugggggagu gaugauuuuc     2040

cugagcaccg ccgucucugc u                                               2061


<210>  2409
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short CDS opt

<400>  2409
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gaucaagguu      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggugg cggucgccau ugcuuggcuc      480

cugggcagcu cuacguccca gaaggugauc uaccuuguua ugauucugcu caucgccccu      540

gcuuauagca uucgcugcau cggggugucu aacagagacu uugucgaagg aauguccggc      600

gggacauggg uggauguggu ccuggagcac ggaggcugug uuaccgugau ggcccaggac      660

aaacccacag uggauauuga acuugucacc accacaguga gcaauauggc ggagguucgg      720

ucuuacugcu acgaggccuc caucagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ugugcaaaag aacacucguc      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu gaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcauuaugc uguccguuca cggaagccaa cauucuggca ugaucgugaa cgacauuggg     1020

cacgagacgg augaaaauag agccaagguc gaggugacac cuaacagccc gcgggccgag     1080

gcuacccuug gaggcuucgg cucccugggg cucgacugug aacccagaac cggacuggau     1140

uuuucugacc uuuacuaucu gacaaugaau aacaaacauu ggcucgucca caaggagugg     1200

uuccacgaua ucccucugcc cuggcaugcg ggcgccgaca ccgggacacc ucacuggaau     1260

aacaaagaag cucuggugga guuuaaggau gcccaugcca aacgccagac gguugugguc     1320

cucggaagcc aggaaggcgc ugugcacacc gcccuggcgg gggcucuuga ggccgagaug     1380

gacggcgcca agggacggcu guucuccggc caucucaagu gcagacugaa aauggauaaa     1440

cuucgccuga aggggguuag cuacucucuc uguacagcug ccuuuaccuu cacaaaggug     1500

cccgcugaaa cccugcacgg aacggucacc guggaggugc aguaugccgg cacagacggg     1560

ccgugcaaag uuccugcgca aauggccguc gacaugcaga cccugacacc cgugggacgg     1620

cucauuaccg cuaauccugu gaucacggaa uccacagaga acagcaagau gaugcuggaa     1680

cuugauccuc ccuuuggcga cucuuacauc gucauugggg ugggcgauaa gaaaaucacc     1740

caccauuggc acagauccgg aagcacaauu ggcaaggccu ucgaggcuac cguucgcggg     1800

gccaaacgga uggcggugcu gggagacacc gccugggauu ucggcuccgu cgggggagug     1860

uuuaacucuc ucggcaaggg gauccaucag auuuucggcg cugccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug aucgggacac uucucguuug gcugggacug     1980

aauacgaaga acggcagcau uucccucacc ugucuggcuc uugggggagu gaugaucuuc     2040

cugagcacag ccgucucugc c                                               2061


<210>  2410
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short CDS opt

<400>  2410
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaacc ugggggugaa uaaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacca uguccuauga augccccaug cuggacgagg gcguugaacc cgaugacgug      240

gauuguuggu gcaacacaac cucuacaugg guggucuacg ggaccuguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcugug acgcuuccgu cccacucuac ccggaagcug      360

caaacacgca gccagaccug gcucgaaucc agagaguaua caaaacaucu gaucaagguu      420

gagaauugga ucuuucggaa ccccggcuuc gcccuggcgg ccguggcuau ugccuggcuc      480

cugggcagcu cuaccuccca gaaggucauc uaccuuguga ugauucugcu caucgcuccu      540

gccuauagca uucgcugcau cgggguuucu aauagagacu uuguggaagg aauguccggc      600

gggacguggg ucgauguggu gcuggagcac ggaggcugug ucacaguuau ggcgcaggac      660

aaacccaccg uggauauuga acuugugaca accaccguca gcaacauggc cgaggugcgg      720

ucuuacugcu acgaggcuuc caucagcgac auggccuccg auucucgcug uccuacacaa      780

ggggaggcuu aucuggacaa gcagagcgac acgcaguacg uuugcaaaag aacccucgug      840

gaucggggau ggggcaaugg cugugggcug uucggaaagg gcucccuggu cacaugcgcc      900

aaauuuaccu gcagcaagaa gaugacaggg aaaucuaucc agcccgaaaa ccucgaauau      960

cgcauuaugc uguccgugca cggaagccaa cauucuggca ugaucguuaa ugacgagaac     1020

agagccaagg uggaagucac cccuaauagc ccgcgggcug aggcgacgcu ugggggauuc     1080

ggcucccugg gccucgauug ugagcccaga acagggcugg acuuuucuga ucuuuacuau     1140

cugaccauga acaauaaaca cuggcucgug cauaaggaau gguuccacga cauuccucug     1200

cccuggcacg ccggagcuga uaccggcaca ccucauugga acaauaaaga ggcccugguc     1260

gaauuuaagg acgcccacgc uaaacgccag accgugguug ugcucgggag ccaggaggga     1320

gccguccaua cagcgcuggc uggcgcccuu gaagccgaga uggauggggc uaagggccgg     1380

cuguucuccg gacaccucaa gugcagacug aaaauggaca aacuucgccu gaagggcgug     1440

agcuacucuc ucuguacggc cgcuuuuacc uucacaaagg uucccgccga gacccugcau     1500

gggacaguga ccgucgaagu gcaguaugcg ggaacggaug gcccgugcaa agugccugcc     1560

caaauggcug uugacaugca gacccugaca cccgucgggc ggcucaucac cgccaacccu     1620

gugauuacag aguccaccga aaacagcaag augaugcugg agcuugaccc ucccuuugga     1680

gauucuuaca ucgugaucgg cgucggggac aagaaaauua cgcaccacug gcauagaucc     1740

ggcagcacaa ucggaaaggc uuucgaagcc accgugcgcg gcgcgaaacg gauggccguu     1800

cugggggaua cagcuuggga cuucggaucc gugggcgggg ucuuuaauuc ucucggaaag     1860

ggcauucacc agaucuucgg ggccgcuuuu aaaagccugu ucggcggaau guccugguuu     1920

ucucaaauuc ugaucggcac ccuucucgug uggcuggggc ugaacaccaa gaauggaagc     1980

aucucccuca caugucuggc ccuuggcggg guuaugauuu uccugagcac ggccgugucu     2040

gcu                                                                   2043


<210>  2411
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short CDS opt

<400>  2411
accagcaucg gcauuguggg gcugcuccug acaacggcca uggcugccga gaucaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc cauuagcuuc      120

gccacaaccc ugggggucaa caaaugccac guucagauca uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguggaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcuguu acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gauuaaggug      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggucg cgguggccau cgcuuggcuc      480

cugggcagcu cuacguccca gaagguuauu uaccuuguga ugauccugcu cauugccccu      540

gcuuauagca uccgcugcau uggggucucu aacagagacu uuguggaagg aauguccggc      600

gggacauggg uggaugucgu ucuggagcac ggaggcugug ugaccgugau ggcccaggac      660

aaacccacag ucgauaucga acuugugacc accacaguua gcaauauggc ggaggugcgg      720

ucuuacugcu acgaggccuc cauuagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ucugcaaaag aacacucgug      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu uaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcaucaugc uguccgugca cggaagccaa cauucuggca ugauugucaa cgacacgggg     1020

cacgagacag augaaaauag agccaaggug gaggucaccc cuaacagccc gcgggccgag     1080

gcuacccuug gaggcuucgg cucccugggg cucgacugug aacccagaac aggacuggau     1140

uuuucugacc uuuacuaucu gaccaugaau aacaaacauu ggcucgugca caaggagugg     1200

uuccacgaua ucccucugcc cuggcaugcg ggcgccgaca cagggacgcc ucacuggaau     1260

aacaaagaag cucugguuga guuuaaggau gcccaugcca aacgccagac cguggucgug     1320

cucggaagcc aggaaggcgc uguucacaca gcccuggcgg gggcucuuga ggccgagaug     1380

gacggcgcca agggacggcu guucuccggc caucucaagu gcagacugaa aauggauaaa     1440

cuucgccuga agggggugag cuacucucuc uguaccgcug ccuuuacauu caccaagguc     1500

cccgcugaaa cgcugcacgg aaccgugaca guggagguuc aguaugccgg caccgacggg     1560

ccgugcaaag ucccugcgca aauggccgug gacaugcaga cacugacccc cgugggacgg     1620

cucauuacgg cuaauccugu caucacagaa uccaccgaga acagcaagau gaugcuggaa     1680

cuugauccuc ccuuuggcga cucuuacauu gugaucgggg uuggcgauaa gaaaaucaca     1740

caccauuggc acagauccgg aagcaccauu ggcaaggccu ucgaggcuac cgugcgcggg     1800

gccaaacgga uggcgguccu gggagacaca gccugggauu ucggcuccgu ugggggagug     1860

uuuaacucuc ucggcaaggg gauccaucag auuuucggcg cugccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccug auugggacgc uucucgugug gcugggacug     1980

aauaccaaga acggcagcau cucccucaca ugucuggcuc uugggggagu caugaucuuc     2040

cugagcaccg ccgugucugc c                                               2061


<210>  2412
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short CDS opt

<400>  2412
accagcaucg gcauuguggg gcugcuccug acaacggcca uggcugccga gaucaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc cauuagcuuc      120

gccacaaccc ugggggucaa caaaugccac guucagauca uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguggaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguacg ggacguguca acacaagaaa      300

ggagaggcca gacggagccg cagagcuguu acccuuccgu cccauucuac acggaagcug      360

cagacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaccu gauuaaggug      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggucg cgguggccau cgcuuggcuc      480

cugggcagcu cuacguccca gaagguuauu uaccuuguga ugauccugcu cauugccccu      540

gcuuauagca uccgcugcau uggggucucu aacagagacu uuguggaagg aauguccggc      600

gggacauggg uggaugucgu ucuggagcau ggaggcugug ugaccgugau ggcccaagac      660

aaacccacag ucgauaucga acuugugacc accacaguua gcaauauggc ggaggugcgg      720

ucuuacugcu acgaggccuc cauuagcgac auggcuuccg auucucgcug uccuacgcag      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ucugcaaaag aacacucgug      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu uaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc aacccgagaa ucucgaauau      960

cgcaucaugc uguccgugca cggaagccag cacucuggca ugauugucaa cgacaucggg     1020

caugagacgg augaaaauag agccaaggug gaggucacac cuaacagccc gcgggccgag     1080

gcuacccuug gaggcuucgg cucccugggg cucgacugug aacccagaac cggacuggau     1140

uuuucugacc uuuacuaucu gacaaugaau aacaaacacu ggcucgugca uaaggagugg     1200

uuccacgaua uuccucugcc cuggcacgcg ggcgccgaca ccgggacacc ucauuggaau     1260

aacaaagaag cucugguuga guuuaaggau gcccacgcca aacgccagac gguggucgug     1320

cucggaagcc aggaaggcgc uguucauacc gcccuggcgg gggcucuuga ggccgagaug     1380

gacggcgcca agggacggcu guucuccggc caccucaagu gcagacugaa aauggauaaa     1440

cuucgccuga agggggugag cuacucucuc uguacagcug ucugcaccgc cgcuaaggug     1500

cccgccgaaa cacugcaugg aaccgugacg guugaggucc aauaugcggg caccgacggg     1560

ccguguaaag ugccugccca gauggcugug gacaugcaga cacugacccc cgucggacgg     1620

cucaucacag ccaauccugu gauuaccgaa uccacggaga acagcaagau gaugcuggaa     1680

cuugauccuc ccuuuggcga cucuuacauc guuaucgggg ugggcgauaa gaaaauuaca     1740

caccacuggc auagauccgg aagcaccauc ggcaaggcuu ucgaggccac aguccgcggg     1800

gcgaaacgga uggccgugcu gggagacacc gcuugggauu uuggcuccgu ugggggagug     1860

uucaacucuc ucggcaaggg gauucaccaa aucuucggcg ccgcuuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucagauucug aucgggaccc uucucgucug gcugggacug     1980

aauacaaaga acggcagcau cucccucacg ugccuggccc uugggggagu gaugauuuuc     2040

cugagcaccg ccguuucugc u                                               2061


<210>  2413
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short CDS opt

<400>  2413
accagcaucg gcauuguggg gcugcuccug acaacggcca uggcugccga gaucaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc cauuagcuuc      120

gccacaaccc ugggggucaa caaaugccac guucagauca uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguggaacc cgaugacgug      240

gauuguuggu gcaauaccac aucuaccugg gucguguacg ggacguguca ccauaagaaa      300

ggagaggcca gacggagccg cagagcuguu acccuuccgu cccacucuac acggaagcug      360

caaacccgca gccagacaug gcucgaaucc agagaguaua ccaaacaucu gauuaaggug      420

gagaacugga ucuuucggaa ucccggcuuc gcccuggucg cgguggccau cgcuuggcuc      480

cugggcagcu cuacguccca gaagguuauu uaccuuguga ugauccugcu cauugccccu      540

gcuuauagca uccgcugcau uggggucucu aacagagacu uuguggaagg aauguccggc      600

gggacauggg uggaugucgu ucuggagcac ggaggcugug ugaccgugau ggcccaggac      660

aaacccacag ucgauaucga acuugugacc accacaguua gcaauauggc ggaggugcgg      720

ucuuacugcu acgaggccuc cauuagcgac auggcuuccg auucucgcug uccuacgcaa      780

ggggaagccu aucuggacaa gcagagcgac acccaguacg ucugcaaaag aacacucgug      840

gaucggggau ggggcaacgg cugugggcug uucggaaagg gcucccuggu uaccugcgcu      900

aaauuuacau gcagcaagaa gaugaccggg aaaucuaucc agcccgagaa ucucgaauau      960

cgcaucaugc uguccgugca cggaagccaa cauucuggca ugauugucaa cgacgagaau     1020

agagccaagg uggaagucac gccuaacagc ccgcgggccg aggcuacacu ugggggauuc     1080

ggcucccugg gccucgauug ugagcccaga accgggcugg acuuuucuga ucuuuacuau     1140

cugaccauga auaacaaaca cuggcucgug cauaaggaau gguuccacga caucccucug     1200

cccuggcacg cgggagccga uacaggcacc ccucauugga auaacaaaga ggcucugguu     1260

gaauuuaagg acgcccacgc caaacgccag acaguggucg ugcucgggag ccaggaggga     1320

gcuguucaua cggcccuggc gggcgcucuu gaagccgaga uggauggggc caagggccgg     1380

cuguucuccg gacaccucaa gugcagacug aaaauggaca aacuucgccu gaagggcgug     1440

agcuacucuc ucuguaccgc ugccuuuaca uucaccaagg uccccgcuga gacacugcau     1500

gggaccguga cgguggaagu ucaguaugcc ggaaccgaug gcccgugcaa agucccugcg     1560

caaauggccg uggacaugca gacacugacc cccguggggc ggcucauuac agcuaauccu     1620

gucaucaccg aguccacgga aaacagcaag augaugcugg agcuugaccc ucccuuugga     1680

gauucuuaca uugugaucgg cguuggggac aagaaaauca cacaccacug gcauagaucc     1740

ggcagcacca uuggaaaggc cuucgaagcu acagugcgcg gcgccaaacg gauggcgguc     1800

cugggggaua ccgccuggga cuucggaucc gugggcgggg uuuuuaacuc ucucggaaag     1860

ggcauccacc agauuuucgg ggcugccuuu aaaagccugu ucggcggaau guccugguuu     1920

ucucaaaucc ugauuggcac ccuucucgug uggcuggggc ugaauacaaa gaacggaagc     1980

aucucccuca cgugucuggc ucuuggcggg gucaugaucu uccugagcac cgccgugucu     2040

gcc                                                                   2043


<210>  2414
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short CDS opt

<400>  2414
accagcaucg gcauuaucgg gcugcuccug acaacggcca uggcugccga gauuaccaga       60

cggggauccg cguacuauau guaccuugac cgcucugaug cuggcaaggc caucagcuuc      120

gccacaaccc ugggggugaa caaaugccac guccagauua uggaccucgg acauaugugu      180

gaugcuacaa uguccuauga augccccaug cuggacgagg gcguugaacc ugaugacgug      240

gauuguuggu gcaauaccac aucuaccugg guggucuacg ggacguguca ccauaagaaa      300

ggagagacca gacggagccg cagauccgug ucucuucggu aucacuacac acgcaagcug      360

caaaccagaa gccagacaug gcucgaaucc cgggaguaua aaaagcaucu gaucaugguu      420

gagaacugga ucuuucgcaa uccgggcuuc gccauuguga gcgucgcuau caccuggcug      480

augggcucuc ucacguccca gaaggugauu uaccugguua ugaucgugcu uauugucccc      540

gccuacagca ucucuugcau uggggugucc aacagagacc ugguggaagg aaugagcggc      600

gggacauggg ucgauguugu gcucgagcac ggaggcugug ugaccgaaau ggcgcaggac      660

aaaccuacag ucgauaucga gcuggugacc augaccguuu cuaauauggc cgaggugcgg      720

uccuauugcu acgaagcuag ccuuuccgac auggccucug cuagccgcug ucccacacaa      780

ggggagccuu cccuggauaa gcagagcgac acgcagucug ucugcaaaag aacccucgga      840

gaccggggcu ggggcaacgg guguggaauc uuuggcaagg ggucccuggu gacaugcagc      900

aaauuuaccu gcuguaagaa gaugcccgga aaaucuauuc agccugaaaa ucuggaguau      960

cgcaucaugc ucccgguuca cggcagccaa cauuccggga ugauugugaa cgauaucgga     1020

cacgaaacag acgagaauag agccaagguc gaggugaccc ccaacucucc ucgggcggaa     1080

gccacgcugg gcggcuuugg gagccuugga cuggauugcg agcccagaac aggccucgac     1140

uucuccgauc uguacuaucu uaccaugaau aacaaacauu ggcuggucca caaggaaugg     1200

uuucacgaca uuccucuccc cuggcaugcu ggggccgaua ccggaacacc gcacuggaau     1260

aacaaagagg cucuggugga guucaaggac gcccaugcca aacgccagac cguugugguc     1320

cugggcagcc aggaaggggc ugugcacaca gcgcucgccg gcgcucugga ggccgaaaug     1380

gauggagcca agggccggcu uuucucuggg caucugaagu guagacucaa aauggacaag     1440

cugcgccuua aaggaguuuc cuacagccug ugcacggcug ccuucaccuu uacaaaggug     1500

ccugcggaga cccuccacgg cacagucacc guggaagugc agucugcugg gacggaugga     1560

cccuguaaag uuccugccca aauggccguc gacaugcaga cccugacacc cgugggccgg     1620

cugaucaccg cuaaucccgu gaucacagag uccaccgaaa acagcaagau gaugcucgag     1680

cuggacccuc cguucgggga uuccuauauu gucaucggcg ugggagacaa gaaaauuacg     1740

caccauuggc acagaucugg cagcacaauc gggaaggccu uugaggcuac cguucgcgga     1800

gccaaacgga uggcggugcu uggcgauaca gccugggacu ucggguccgu cggaggcgug     1860

uucaacucuc uggggaaggg cauucaucag aucuuuggag cugccuucaa aagccucuuu     1920

ggcgggaugu ccugguucag ccaaauccug auuggaaccc ugcuuguuug gcucggccug     1980

aauaccaaga acgggucuau cucccugaca ugccucgcuc ugggaggcgu gaugauuuuu     2040

cuuagcacgg ccgucucugc g                                               2061


<210>  2415
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short CDS opt

<400>  2415
agcgugggca ucgucgggcu gcuccugacc acagccaugg cugccgaggu uacgagacgg       60

ggauccgcgu acuauaugua ccuugaccgc aacgaugcug gcgaagccau uucuuucccc      120

accacacugg ggaugaauaa gugcuauauc cagauuaugg accucggaca caugugugau      180

gccaccauga gcuacgagug cccuaugcug gacgaaggcg uggagccgga ugacguggau      240

uguuggugca acacaaccuc cacauggguc guguauggga ccugucauca caaaaaggga      300

gaagcuagac ggucucgcag agccguuacg cuucccagcc auuccacccg gaaacugcaa      360

acacgcucuc agaccuggcu cgagagcaga gaguacacaa agcaccugau ccggguggaa      420

aauuggauuu uucgcaaccc uggcuucgcu cuggccgcgg ccgcuaucgc cuggcuccug      480

ggcuccagca ccucucagaa agucaucuau cuugugauga uucugcucau cgcucccgcc      540

uacuccauua gaugcaucgg gguuagcaau cgggacuuug uggagggaau gucuggcggg      600

acgugggucg auguggugcu ggaacaugga ggcuguguca caguuauggc gcaggacaag      660

ccuaccgugg auauugagcu ugugacaacc accgucucca acauggccga ggugcgcagc      720

uacugcuaug aagccucuau cuccgacaug gcuagcgauu ccagaugucc cacacaaggg      780

gaggcuuacc uggacaagca gucugacacg caguauguuu gcaaacggac ccucguggau      840

cgcggauggg gcaauggcug ugggcuguuc ggaaagggca gccuggucac augcgccaaa      900

uuugccugcu ccaagaaaau gaccggaaag agcauucagc cugaaaaccu cgaguacaga      960

aucaugcugu cugugcacgg gucccaacac agcggcauga ucguuaauga cacagggcau     1020

gaaaccgaug agaaccgggc uaagguggag auuacgccca auucucccag agcggaagcc     1080

acacuuggag gcuucggcag ccuggggcuc gacugugagc cucgcaccgg acuggauuuu     1140

uccgaccuuu auuaccugac caugaacaau aaacacuggc ucguccauaa ggaaugguuc     1200

cacgauaucc cccugccuug gcacgcuggc gccgacacag ggaccccgca uuggaacaau     1260

aaagaggccc ugguggaauu uaaggaugcu cacgccaaac ggcagacagu cgugguucuc     1320

ggaucucagg agggcgcggu gcauacggcu cuggccgggg cccuugaggc ugaaauggac     1380

ggcgccaagg gaagacugag cuccggccac cucaaaugcc gccugaagau ggauaagcug     1440

cggcugaaag gggucagcua uucucucugu accgcugccu ucacauuuac caagauuccg     1500

gcggagacac ugcauggaac cgugacgguu gaagugcagu acgccggcac cgacgggccu     1560

ugcaaagucc ccgcucaaau ggccguggac augcagacac ugaccccugu gggaagacuc     1620

aucacagcua accccguuau uaccgagucc acggaaaaca gcaagaugau gcuggagcuu     1680

gaucccccuu ucggcgacuc uuacaucguc aucggggugg gcgagaaaaa gauuacacac     1740

cacuggcauc gcuccggaag caccaucggc aaggccuuug aagcgacagu gagaggggcc     1800

aaacggaugg cuguccuggg agauaccgcc ugggacuucg gcuccguugg gggagcucuc     1860

aauucucugg gcaaggggau ucaccagauc uucggcgccg ccuuuaaaag ccuguucgga     1920

ggcauguccu gguuuucuca aauucuuauc gggacccucc ugauguggcu gggacucaac     1980

acaaagaaug gcagcaucuc ccugaugugu cuugcucuug ggggagugcu cauuuuccug     2040

agcacggcgg ugucugcc                                                   2058


<210>  2416
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short CDS opt

<400>  2416
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauauuga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag caucuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagauuacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauu     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucaucaccg cuaaccccgu uauuacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuacc     1740

caccacuggc aucgcuccgg aagcacaauc ggcaaggccu uugaagcgac cgugagaggg     1800

gcccggcgca uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaagg gauucaccag aucuucggcg ccgccuuuaa gagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggacac uccugaugug gcugggacuc     1980

aacacgaaaa auggcagcau cucccugaug ugucuugcuc uugggggagu ucucauuuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2417
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short CDS opt

<400>  2417
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uguuucgcaa cccuggcuuc gcucuggccg cggccgcuau ugccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauccugcu cauugcuccc      540

gccuacucca ucagaugcau ugggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccguuau ggcgcaggac      660

aagccuacag uggauaucga gcuugugacc accacagucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagcuag cauuuccgac auggccagcg auuccagaug ucccacgcaa      780

ggggaggcuu accuggacaa gcagucugac acccaguaug uuugcaaacg gacacucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugccu gcuccaagaa aaugacaggg aagucuaucc agccugaaaa ccucgaguac      960

agaauuaugc ugucugugca cggaucccaa cacagcggca ugaucguuaa ugacaccggg     1020

caugaaacgg augagaaccg ggcuaaggug gagaucacac ccaauucucc gagagcggaa     1080

gccacccuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gacaaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcu ggcgccgaca ccgggacacc ccauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac ggucgugguu     1320

cucggaucuc aggagggcgc ggugcauacc gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguacagcug ccuucaccuu uacaaagauc     1500

ccggcggaga cccugcaugg aacggugacc guugaagugc aguacgccgg cacagacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cccugacacc ugugggaaga     1620

cucauuaccg cuaaccccgu uaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauu gucaucgggg ugggcgagaa aaagaucacc     1740

caccacuggc aucgcuccgg aagcacaauu ggcaaggccu uugaagcgac cgugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu cgggggagcu     1860

cucaauucuc ugggcaaggg gauccaccag auuuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauccuu auugggacac uccugaugug gcugggacuc     1980

aacacgaaga auggcagcau cucccugaug ugucuugcuc uugggggagu ucucaucuuc     2040

cugagcaccg cggugucugc c                                               2061


<210>  2418
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short CDS opt

<400>  2418
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaugac cuccacaugg gucguguaug ggaccuguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acgcuuccca gccauuccac ccggaaacug      360

caaacacgcu cucagaccug gcucgagagc agagaguaca caaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcaccucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacguggg ucgauguggu gcuggaacau ggaggcugug ucacaguuau ggcgcaggac      660

aagccuaccg uggauauuga gcuugugaca accaccgucu ccaacauggc cgaggugcgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacacaa      780

ggggaggcuu accuggacaa gcagucugac acgcaguaug uuugcaaacg gacccucgug      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu cacaugcgcc      900

aaauuugccu gcuccaagaa aaugaccgga aagagcauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugugca cgggucccaa cacagcggca ugaucguuaa ugacacaggg     1020

caugaaaccg augagaaccg ggcuaaggug gagauuacgc ccaauucucc cagagcggaa     1080

gccacacuug gaggcuucgg cagccugggg cucgacugug agccucgcac cggacuggau     1140

uuuuccgacc uuuauuaccu gaccaugaac aauaaacacu ggcucgucca uaaggaaugg     1200

uuccacgaua ucccccugcc uuggcacgcu ggcgccgaca cagggacccc gcauuggaac     1260

aauaaagagg cccuggugga auuuaaggau gcucacgcca aacggcagac agucgugguu     1320

cucggaucuc aggagggcgc ggugcauacg gcucuggccg gggcccuuga ggcugaaaug     1380

gacggcgcca agggaagacu gagcuccggc caccucaaau gccgccugaa gauggauaag     1440

cugcggcuga aaggggucag cuauucucuc uguaccgcug ccuucacauu uaccaagauu     1500

ccggcggaga cacugcaugg aaccgugacg guugaagugc aguacgccgg caccgacggg     1560

ccuugcaaag uccccgcuca aauggccgug gacaugcaga cacugacccc ugugggaaga     1620

cucaucacag cuaaccccgu uauuaccgag uccacggaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauc gucaucgggg ugggcgagaa aaagauuaca     1740

caccacuggc aucgcuccgg aagcaccauc ggcaaggccu uugaagcgac agugagaggg     1800

gccaaacgga uggcuguccu gggagauacc gccugggacu ucggcuccgu ugggggagcu     1860

cucaauucuc ugggcaaggg gauucaccag aucuucggcg ccgccuuuaa aagccuguuc     1920

ggaggcaugu ccugguuuuc ucaaauucuu aucgggaccc uccugaugug gcugggacuc     1980

aacacaaaga auggcagcau cucccugaug ugucuugcuc uugggggagu gcucauuuuc     2040

cugagcacgg cggugucugc c                                               2061


<210>  2419
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short CDS opt

<400>  2419
accagcgugg gcaucgucgg gcugcuccug acaacggcca uggcugccga gguuaccaga       60

cggggauccg cguacuauau guaccuugac cgcaacgaug cuggcgaagc cauuucuuuc      120

cccacaaccc uggggaugaa uaagugcuau auccagauua uggaccucgg acacaugugu      180

gaugccacaa ugagcuacga gugcccuaug cuggacgaag gcguggagcc ggaugacgug      240

gauuguuggu gcaacaccac auccaccugg gucguguaug ggacguguca ucacaaaaag      300

ggagaagcua gacggucucg cagagccguu acccuuccca gccauuccac acggaaacug      360

caaacccgcu cucagacaug gcucgagagc agagaguaca ccaagcaccu gauccgggug      420

gaaaauugga uuuuucgcaa cccuggcuuc gcucuggccg cggccgcuau cgccuggcuc      480

cugggcucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcuccc      540

gccuacucca uuagaugcau cgggguuagc aaucgggacu uuguggaggg aaugucuggc      600

gggacauggg ucgauguggu gcuggaacau ggaggcugug ucaccgcgau ggcccaggac      660

aagccuacag uugauauuga gcuugugacc accacagugu ccaacauggc ugagguccgc      720

agcuacugcu augaagccuc uaucuccgac auggcuagcg auuccagaug ucccacgcaa      780

ggggaggccu accuggacaa gcagucugac acccaguaug ugugcaaacg gacacucguu      840

gaucgcggau ggggcaaugg cugugggcug uucggaaagg gcagccuggu gaccugcgcc      900

aaauuugcuu gcuccaagaa aaugacaggg aagucuauuc agccugaaaa ccucgaguac      960

agaaucaugc ugucugucca cggaucccaa cacagcggca ugcuugugaa ugacaccggg     1020

caugaaacgg augagaaccg ggcgaagguu gagaucacac cgaauagccc cagagccgaa     1080

gcuacccugg gaggcuucgg cagccucggg cuggacugug agccucgcac cggacuugau     1140

uuuuccgacc uguauuaccu cacaaugaac aauaaacacu ggcuggccca uaaggaaugg     1200

uuccacgaua uuccccugcc uuggcacgcc ggcgcugcca ccgggacacc ccauuggaac     1260

aauaaagagg cgcucgugga auuuaaggac gcucacgcca aacggcagac ggucgugguc     1320

cugggaucuc aggagggcgc cgugcauacc gcucuugccg gggcucugga ggccgaaaug     1380

gauggcgcga agggaagacu cagcuccggc caccugaaau gccgccuuaa gauggacaag     1440

cugcggcuca aagggguuag cuauucucug uguacagccg cuuucaccuu uacaaagauc     1500

ccggccgaga ccguggaugg aacggucacc guggaaggcc aguacggggg aacagacggc     1560

ccuugcaaag uucccgcuca aauggccgug gacaugcaga cccugacacc ugucgggaga     1620

cucauuaccg cgaaccccgu gaucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugaucccc cuuucggcga cucuuacauu gugaucggag uuggcgagaa aaagaucacc     1740

caucacuggc accgcuccgg gagcacaauu ggaaaggccu uugaagcuac cguccggggc     1800

gccaaaagaa uggcugugcu gggggauacc gccugggacu ucggauccgu gggcggggcc     1860

cucaauucuc ugggcaaggg aauccaucag auuaucggcg cugcguucaa aagccuguuu     1920

gggggaaugu ccugguucuc ucaaauucuu aucggcacac uccugaugug gcuggggcuc     1980

aacacgaaga auggaagcau cucccugaug ugucuugccc ugggcggggu ccucauuuuu     2040

cugagcaccg cugugucugg c                                               2061


<210>  2420
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short CDS opt

<400>  2420
ggcgccgacg cuagcaucgg gauugugucc cugcuccuga ccacagucau ggccgcggag       60

aucacgagac ggggaucugc uuacuauaug uaccuugauc gcagcgacgc cggcaaggcc      120

auuuccuucg uuaccacacu gggggugaac aaaugccacg ugcagaucau ggaucucgga      180

cauaugugug acgcuaccau gucuuaugaa ugccccaugc uggaugaggg cgucgaaccc      240

gacgaugugg acuguuggug caauacaacc agcacauggg uuguguacgg gaccugucac      300

cauaagaaag gagaggccag acggucccgc agagcuguca cgcuuccguc ucacagcacc      360

cggaagcugc aaacacgcuc ccagaccugg cucgaaagca gagaguauac aaaacaucug      420

auuaaggugg agaacuggau cuuucggaau cccggcuucg cccugguugc gguggccauc      480

gcuuggcucc ugggaucuuc caccagccag aaggucauuu accuugugau gauccugcuc      540

auugccccug cuuauucuau ccgcugcauu ggggugucca acagagauuu ugucgaagga      600

augagcggcg ggacgugggu ugacguggug cuggagcacg gaggcugugu cacagugaug      660

gcccaggaua aacccaccgu ugacaucgaa cuugugacaa ccaccgucuc uaauauggcg      720

gaggugcggu ccuacugcua cgaggccagc auuuccgaua uggcuucuga cagccgcugu      780

ccuacacaag gggaagccua ucuggacaag caguccgaua cgcaguacgu uugcaaaaga      840

acccucgugg accggggaug gggcaacggc ugugggcugu ucggaaaggg cagccugguc      900

acaugcgcua aauuuaccug cucuaagaag augacaggga aauccaucca gcccgagaau      960

cucgaauauc gcaucaugcu gagcgugcac ggaucucaac auagcggcau gauugucaac     1020

gaugagaaua gagccaaggu ggaaguuacc ccuaacuccc cgcgggccga ggcuacgcuu     1080

gggggauucg gcucucuggg ccucgacugu gagcccagaa cagggcugga uuuuagcgac     1140

cuuuacuauc ugaccaugaa uaacaaacac uggcucgugc auaaggaaug guuccacgau     1200

aucccucugc ccuggcacuc cggagcggac accgagacac cucauuggaa uaacaaagaa     1260

gcccuggucg aguuuaagga ugcucacgcc aaacgccaga ccgugguugu gcucggcagc     1320

caggaagggg ccguccauac agcucuggcc ggagcgcuug aggcugagau ggacggcgcc     1380

aaggggcggc ugucuuccgg ccaccucaag ugcagacuga aaauggauaa acuucgccug     1440

aagggaguga gcuacucucu cuguacggcc gcuuucaccu uuacaaaggu gcccgccgaa     1500

acccugcaug gcacaguuac cgucgaggug caguaugcug ggcgggacgg accgugcaaa     1560

gugccugccc aaauggcggu cgacaugcag acgcugaccc ccgugggcag acucauuaca     1620

gccaauccug uuaucaccga auccacagag aacagcaaga ugaugcugga acuugauccu     1680

cccuucgggg acuccuacau ugugaucgga gucggcgaua agaaaaucac ccaccacugg     1740

caucgcucug ggagcauuau cggcaaggcu uuugaggcca cggugcgggg agcuaaaaga     1800

auggccguuc ugggcgacac agcgugggau uucggguccg ugggaggcgu cuucaacucu     1860

cucgggaagg gcauucacca gaucuuuggc gccgcuuuca aaagccuguu ugggggcaug     1920

uccugguuca gccaaauucu gaucggaacc cuucucgugu ggcugggccu gaauacaaag     1980

aacgggucua ucucccucac cugucuggcc cuuggaggcg uuaugauuuu ucugagcacc     2040

gcugugucug cc                                                         2052


<210>  2421
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short CDS opt

<400>  2421
ggcgccgaca ccagcaucgg gauuaucgga cugcuccuga caacggcuau ggccgcggag       60

auuaccagac ggggcuccgc uuacuauaug uaccuugauc gcucugacgc cgggaaggcc      120

aucagcuucg cuacaaccau gggagugaac aaaugccacg uccagauuau ggaucugggc      180

cauaugugug acgccacaau guccuaugaa ugccccaugc ucgaugaggg gguugaaccu      240

gacgaugugg acuguuggug caauaccaca ucuaccuggg uggucuacgg aacgugucac      300

cauaagaaag gcgaggcuag accgagccgg cgcgccguga cccugcccuc ccacucuaca      360

agaaaacuuc aaacccggag ccagacaugg cuggaauccc gcgaguauac caagcaucuc      420

aucaagguug agaacuggau ccugagaaau ccuggcuuua cgguggcggc ccuggcuaug      480

gccuggcucc uggggaacag cacaucucag cgggucauuu accuugugau gauccugcuc      540

auugcucccg ccuauuccau ccgcugcauu ggaguuagca auagagauuu cguggaaggc      600

augucugggg gaaccugggu cgacguggug cuggagcacg gcgggugugu cacaguuaug      660

gcgcaggaua agccuaccgu ggacaucgaa cuugugacca caacggucuc caacauggcc      720

gaggugcgga gcuacugcua cgaggcuucu auuuccgaua uggccagcga cucccgcugu      780

cccacccaag gagaggcuua ucuggacaaa cagucugaua cacaguacgu uugcaagaga      840

acccucgugg accggggcug gggcaauggg uguggacugu uuggcaaagg gagccugguc      900

acaugcgcca aguuuaccug cuccaaaaag augacgggaa agagcaucca gccugaaaac      960

cucgaauauc gcaucaugcu gucugugcac ggcucccaac auagcgggau gauuguuaau     1020

gauaucggac acgagacaga cgaaaacaga gccaaagugg aggucacccc caauucuccc     1080

cgggcugagg cgacccuugg cggcuucggg agccugggac ucgauuguga accuagaaca     1140

ggccuggacu ucuccgaucu uuacuaucug accaugaaca auaagcauug gcucgugcac     1200

aaagaguggu uucacgacau uccccugccu uggcaugccg gggcugauac aggaacgccc     1260

cacuggaaca auaaggaagc ccuggucgag uucaaagacg cccaugcuaa gcgccagacc     1320

gugguugugc ucggcucuca ggaaggggcc guccacacag cgcuggcugg cgcccuugag     1380

gccgagaugg auggagcuaa aggccggcug uuuagcgggc aucucaagug cagaaucaag     1440

auggacaaac ugcgccuuaa gggagugucc uacagccugu guaccgccgc uuucacauuu     1500

ucuaaaguuc cggccgaaac ccuccacggc acggugaccg ucgaggugca guaugcgggg     1560

cgggacggac cuugcaaggu gcccgcccaa auggcuguug auaugcagac acugaccccu     1620

gucggcagac ugauuacagc caaccccauc aucaccgaau ccacggagaa cagccgcaug     1680

augcucgaac uggacccgcc uuucggggau ucuuacauug ugaucggcgu gggagacaaa     1740

aagauuacac accauuggca ccgguccggc agcaccaucg ggaaggcuuu ugaggccaca     1800

gucagaggag cgaaacgcau ggccgugcuu ggcgauaccg cuugggacuu cggguccguu     1860

ggaggcgugu ucaauucucu ggggaagggc guccaucagg uguuuggagc cgcuuucaaa     1920

agccucuuug gcgggauguc cugguucucu caaauucuga ucggaacccu gcuuguuugg     1980

cucggccuga acacaaagaa ugggagcauc ucccuggccu gucucgcccu gggaggcgcu     2040

augauuuuuc uuagcacggc ggugucugcc                                      2070


<210>  2422
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE CDS opt

<400>  2422
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

auuguccuug aacacggggg augugugaca guuauggccc aagacaaacc uacgguggau      180

aucgagcugg ucaccacaac cguguccaau auggcggaag uucgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugugugc aaacggacac uggucgaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauucaaccc gagaaucucg aguacagaau caugcugagc      480

gugcaugggu cucagcacuc cggaaugauu gucaacgaua ccggccauga aacagacgag      540

aaucgggcua agguugaaau caccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacauuccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

guggaguuca aggaugcuca cgccaagcgg cagacagucg ugguucucgg gagccaggaa      840

ggagcggugc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gucagcuacu cccugugcac cgccgcuuuu acauucacga agauccccgc ggagacccuu     1020

cacgggacag ugaccguuga agugcaauau gccggaacag acggcccuug uaaagucccc     1080

gcucagaugg ccguggauau gcagacccug acgccugucg ggcgccucau uacagccaau     1140

cccgugauca ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucguuau uggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

agcggcucua ccauuggaaa ggcuuucgag gccacagucc ggggcgcgaa acgcauggcu     1320

gugcucgggg acacggccug ggauuuugga agc                                  1353


<210>  2423
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE CDS opt

<400>  2423
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg acacggccug ggauuuugga agc                                  1353


<210>  2424
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE CDS opt

<400>  2424
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc ggcuaugaaa cagaugagga ccgggccaag      540

guugaaguga ccccuaacag cccccgcgcu gaggccacac ucgggggauu uggcucucug      600

ggccuggauu gcgagccuag aaccgggcuu gacuucuccg aucuguacua ccucacgaug      660

aauaacaaac auuggcuggu gcacaaggaa ugguuucaug acauuccccu uccuuggcac      720

gcgggagccg acacaggcac cccgcacugg aauaacaaag aggcucuggu cgaguucaag      780

gaugcccaug cuaagcggca gacagugguu gugcucggga gccaggaagg agccguccac      840

accgcgcugg ccggcgcucu ggaagccgag auggacgggg cuaaaggacg ccucuuuucc      900

ggccaucuga aguguagacu uaaaauggau aagcugcggc ucaaaggcgu gucuuauagc      960

cugugcaccg ccgccuucac auuuacgaag guucccgcug agacccuuca cgggacagug     1020

accgucgaag ugcaauacgc gggaacagac ggcccuugua aaauccccgu ccagauggcc     1080

guggauaugc agacccugac gccuguuggg cgccucauua cagcuaaucc cgugaucacc     1140

gaguccaccg aaaacagcaa gaugaugcug gagcuggacc cgccuuucgg agauucuuau     1200

auugucaucg gcguggggga caagaaaauc acacaccauu ggcacagauc cggcagcacc     1260

auuggaaagg ccuuugaagc cacaguucgg ggcgcuaaac gcauggccgu gcucggggau     1320

acggcguggg acuucggauc u                                               1341


<210>  2425
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE CDS opt

<400>  2425
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg acacggccug ggauuuugga agc                                  1353


<210>  2426
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE CDS opt

<400>  2426
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua caggcuauga aaccgacgag      540

aaucgggcca agguggaagu gacaccuaac agcccccgcg cugaggccac ccucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaacggggc uugacuucuc cgaucuguac      660

uaccucacaa ugaauaacaa acauuggcug guccacaagg aaugguuuca ugacauuccc      720

cuuccuuggc acgcgggagc cgacaccggc acaccgcacu ggaauaacaa agaggcucug      780

guggaguuca aggaugccca ugcuaagcgg cagaccguug ugguccucgg gagccaggaa      840

ggagccgugc acaccgcgcu ggccggcgcu cuggaagccg agauggacgg ggcuaaagga      900

aagcucuuuu ccggccaucu gaaaugucgc cuuaagaugg auaaacugag acucaagggc      960

guuucuuaua gccugugcac agccgccuuc acguuuacca aagugcccgc ugagacacuu     1020

cacgggaccg ucacagugga aguccaauac gcgggaaccg acggcccuug uaagaucccc     1080

gugcagaugg ccguugauau gcagacgcug acaccugugg ggcggcucau uaccgcuaau     1140

cccgucauca ccgaguccac agaaaacagc aagaugaugc uggagcugga cccgccuuuc     1200

ggagauucuu auauugugau cggcguuggg gacaaaaaga ucacccacca uuggcaccgc     1260

uccggcagca caauuggaaa agccuuugaa gccacgguga gaggcgcuaa gcggauggcc     1320

guccucgggg auaccgcgug ggacuucgga ucu                                  1353


<210>  2427
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE CDS opt

<400>  2427
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugaca guuaacgaua ucggcuauga aaccgacgag      540

aaucgggcca agguggaagu gacaccuaac agcccccgcg cugaggccac ccucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaacggggc uugacuucuc cgaucuguac      660

uaccucacaa ugaauaacaa acauuggcug guccacaagg aaugguuuca ugacauuccc      720

cuuccuuggc acgcgggagc cgacaccggc acaccgcacu ggaauaacaa agaggcucug      780

guggaguuca aggaugccca ugcuaagcgg cagaccguug ugguccucgg gagccaggaa      840

ggagccgugc acaccgcgcu ggccggcgcu cuggaagccg agauggacgg ggcuaaagga      900

aagcucuuuu ccggccaucu gaaaugucgc cuuaagaugg auaaacugag acucaagggc      960

guuucuuaua gccugugcac agccgccuuc acguuuacca aagugcccgc ugagacacuu     1020

cacgggaccg ucacagugga aguccaauac gcgggaaccg acggcccuug uaagaucccc     1080

gugcagaugg ccguugauau gcagacgcug acaccugugg ggcggcucau uaccgcuaau     1140

cccgucauca ccgaguccac agaaaacagc aagaugaugc uggagcugga cccgccuuuc     1200

ggagauucuu auauugugau cggcguuggg gacaaaaaga ucacccacca uuggcaccgc     1260

uccggcagca caauuggaaa agccuuugaa gccacgguga gaggcgcuaa gcggauggcc     1320

guccucgggg auaccgcgug ggacuucgga ucu                                  1353


<210>  2428
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE CDS opt

<400>  2428
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaug aaaaucgggc caagguggag      540

gugacaccua acagcccccg cgcugaagcc acccugggcg gguuuggauc ucucggccug      600

gacugcgagc cuagaacagg ccuugauuuc uccgaccugu auuaccucac caugaauaac      660

aaacauuggc ugguccacaa ggagugguuu caugauauuc cccuuccuug gcacgcgggg      720

gccgacacgg gaacaccgca cuggaauaac aaagaagcuc ugguggaguu caaggacgcc      780

caugcuaagc ggcagaccgu ugugguccuc ggcagccagg aaggggccgu gcacacagcg      840

cuggccggag cucuggaggc cgaaauggau ggcgcuaaag ggcgccucuu uuccggacau      900

cugaagugua gacuuaaaau ggacaagcug cggcucaaag gcguuucuua cagccugugc      960

accgccgccu ucaccuuuac aaaggugccc gcugagacgc uucacggcac cgucacagug     1020

gagguccaau augcggggac cgauggaccu uguaaagugc ccgcccagau ggcuguugac     1080

augcagacac ugaccccugu gggccgccuc aucacggcca aucccgucau uacagaaucc     1140

accgagaaca gcaagaugau gcuggaacug gauccgccuu ucggggacuc uuacaucgug     1200

auuggaguug gcgauaagaa aaucacccac cauuggcaca gauccgggag cacaaucggc     1260

aaggccuuug aggcuaccgu gcggggagcc aaacgcaugg cgguccucgg cgacacagcu     1320

ugggauuucg ggucu                                                      1335


<210>  2429
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE CDS opt

<400>  2429
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc ggcuaugaaa cagaugagaa ccgggccaag      540

guugaaguga ccccuaauag cccccgcgcu gaggccacac ucgggggauu uggcucucug      600

ggccuggacu gcgagccuag aaccgggcuu gauuucuccg accuguacua ccucacgaug      660

aacaauaaac auuggcuggu gcacaaggaa ugguuucaug auauuccccu uccuuggcac      720

gcgggagccg acacaggcac cccgcacugg aacaauaaag aggcucuggu cgaguucaag      780

gacgcccaug cuaagcggca gacagugguu gugcucggga gccaggaagg agccguccac      840

accgcgcugg ccggcgcucu ggaagccgag auggaugggg cuaaaggaaa gcucuuuucc      900

ggccaucuga aaugucgccu uaagauggac aaacugagac ucaagggcgu gucuuauagc      960

cugugcaccg ccgccuucac auuuacgaaa guucccgcug agacccuuca cgggacagug     1020

accgucgaag ugcaauacgc gggaacagau ggcccuugua agauccccgu ccagauggcc     1080

guggacaugc agacccugac gccuguuggg cggcucauua cagcuaaccc cgugaucacc     1140

gaguccaccg aaaauagcaa gaugaugcug gagcuggauc cgccuuucgg agacucuuau     1200

auugucaucg gcguggggga uaaaaagauc acacaccauu ggcaccgcuc cggcagcacc     1260

auuggaaaag ccuuugaagc cacaguuaga ggcgcuaagc ggauggccgu gcucggggac     1320

acggcguggg auuucggauc u                                               1341


<210>  2430
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE CDS opt

<400>  2430
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uucccuuaga       60

ugcauuggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcugg aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

aucgagcucg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcauuu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccug      300

gacaagcagu ccgauaccca guaugucugc aaacggacac uuguggaccg cgggugggga      360

aacggcugug gccuguuugg gaagggaagc cucgugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucugg aguacagaau caugcugagc      480

guccaugggu cucagcacuc cggaaugauu guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau caccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccuuga uugcgagccu agaaccgggc uggacuucuc cgaucucuau      660

uaccugacga ugaauaacaa acacuggcuu gugcauaagg aaugguuuca cgacauuccc      720

cugccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccuc      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcuggg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cucgaagcug agauggacgg ggccaaagga      900

cgccuguccu cuggccaccu uaaguguaga cugaaaaugg auaagcuccg gcugaaaggc      960

gugagcuacu cccuuugcac cgccgcuuuu acauucacga agauccccgc ggagacccug     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccuc acgccugugg ggcgccugau uacagccaau     1140

cccguuauca ccgagagcac cgaaaacucu aagaugaugc uggagcucga cccgccuuuu     1200

ggagauuccu acauugugau cggcgucggg gaaaagaaaa ucacacauca cuggcauaga     1260

agcggcucua ccauuggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucuggggg acacggccug ggauuuugga agc                                  1353


<210>  2431
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE CDS opt

<400>  2431
agcaagaaag ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aggacaagcc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacaaggcga agccuaccuc      300

gacaaacagu ccgauaccca guaugucugc aagcggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaaaggaagc cuggugaccu gcgcuaaguu cgccuguucu      420

aagaaaauga cgggcaaguc cauccagccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucaacacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua aaguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa gcacuggcug gugcauaaag aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa ggaggcccug      780

gucgaguuca aggaugcuca cgccaaacgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaggga      900

cgccucuccu cuggccaccu gaaauguaga cuuaagaugg auaaacugcg gcucaagggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga aaauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaguau gccggaacag acggcccuug uaaggugccc     1080

gcucaaaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gagaaaaaga uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgaa gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg acacggccug ggauuuugga agc                                  1353


<210>  2432
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE CDS opt

<400>  2432
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cugguauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg acacggccug ggauuuugga agc                                  1353


<210>  2433
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE CDS opt

<400>  2433
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

aagcucuccu cuggccaccu gaaaugucgc cuuaagaugg auaaacugag acucaagggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga aaauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaggugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcggcucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gagaaaaaga uuacacauca cuggcaucgc     1260

agcggcucua ccaucggaaa ggcuuucgaa gccacaguga gaggcgcgaa acggauggcu     1320

guucucgggg acacggccug ggauuuugga agc                                  1353


<210>  2434
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE CDS opt

<400>  2434
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauac ccagacgcug acaccugugg ggcgccucau caccgccaau     1140

cccguuauua ccgagagcac agaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

agcggcucua caaucggaaa ggcuuucgag gccacggugc ggggcgcgaa acgcauggcu     1320

guucucgggg acaccgccug ggauuuugga agc                                  1353


<210>  2435
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE CDS opt

<400>  2435
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaaa uccgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugugugc aaacggacac uggucgaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauucaaccc gagaaucucg aguacagaau caugcugagc      480

gugcaugggu cucagcacuc cggaaugauu gucaacgaua ccggccauga aacagacgag      540

aaucgggcua agguugaaau caccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacauuccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

guggaguuca aggaugcuca cgccaagcgg cagacagucg ugguucucgg gagccaggaa      840

ggagcggugc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gucagcuacu cccugugcac cgccgcuuuu acauucacga agauccccgc ggagacccuu     1020

cacgggacag ugaccguuga agugcaauau gccggaacag acggcccuug uaaagucccc     1080

gcucagaugg ccguggauau gcagacccug acgccugucg ggcgccucau uacagccaau     1140

cccgugauca ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucguuau uggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

agcggcucua ccauuggaaa ggcuuucgag gccacagucc ggggcgcgaa acgcauggcu     1320

gugcucgggg acacggccug ggauuuugga agc                                  1353


<210>  2436
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE CDS opt

<400>  2436
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgcg cagaauggcu     1320

guucucgggg acacggccug ggauuuugga agc                                  1353


<210>  2437
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE CDS opt

<400>  2437
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggu cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugugugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cugguuaccu gcgccaaauu cgcuuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc gugaacgaua ccggccauga aacagacgag      540

aaucgggcca agguggaaau uaccccuaac agcccccgcg cugaggccac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug guccauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgcgggagc cgacacaggc accccgcauu ggaauaacaa agaggcucug      780

guggaguuca aggaugccca cgcuaagcgg cagacaguug ugguccucgg gagccaggaa      840

ggagccgugc auaccgcgcu ggccggcgcu cuggaagccg agauggacgg ggcuaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

guuagcuacu cccugugcac cgccgccuuu acauucacga agauucccgc ugagacccuu     1020

cacgggacag ugaccgucga agugcaauau gcgggaacag acggcccuug uaaagucccc     1080

gcccagaugg cuguggauau gcagacccug acgccuguug ggcgccucau cacagccaau     1140

cccgugauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgucau cggcgugggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggccuucgag gcuacaguuc ggggcgccaa acgcauggcg     1320

gugcucgggg acacggcuug ggauuuugga agc                                  1353


<210>  2438
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE CDS opt

<400>  2438
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcau cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau uggcgucggg gaaaagaaaa ucacccauca cuggcauaga     1260

agcggcucua caauuggaaa ggcuuucgag gccaccgugc ggggcgcgaa acgcauggcu     1320

guucucgggg acacagccug ggauuuugga agc                                  1353


<210>  2439
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE CDS opt

<400>  2439
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcauuggg gaaaagaaaa ucacacauca cuggcauaga     1260

agcggcucua ccauuggaaa ggcuuucgag gccacagucc ggggcgcgaa acgcauggcu     1320

gugcucgggg acacggccug ggauuuugga agc                                  1353


<210>  2440
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE CDS opt

<400>  2440
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccuugcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcugg aacacggggg auguguuaca gugauggccc aagacaaacc cacggucgau      180

auugagcucg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccua cacagggcga agccuaccug      300

gacaagcagu ccgauaccca guaugucugc aaacggacac uuguggaccg cgggugggga      360

aacggcugug gccuguuugg gaagggaagc cucgugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccg gagaaucugg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uacccccaac agcccucgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccuuga uugcgagccc agaaccgggc uggacuucuc cgaucucuau      660

uaccugacga ugaauaacaa acacuggcuu gugcauaagg aaugguuuca cgacaucccu      720

cugcccuggc acgccggagc ugacacaggc accccucauu ggaauaacaa agaggcccuc      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcuggg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cucgaagcug agauggacgg ggccaaagga      900

cgccuguccu cuggccaccu uaaguguaga cugaaaaugg auaagcuccg gcugaaaggc      960

gugagcuacu cccuuugcac cgccgcuuuu acauucacga agauuccggc ggagacccug     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggccccug uaaagugccu     1080

gcucagaugg ccgucgauau gcagacccuc acgcccgugg ggcgccugau cacagccaau     1140

ccuguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcucga ucccccguuu     1200

ggagacuccu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucuggggg acacggccug ggauuuugga agc                                  1353


<210>  2441
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE CDS opt

<400>  2441
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gacccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucagcu ccggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugucuuaca gccugugcac agccgcuuuu acguucacca agauucccgc ggagacacuu     1020

cacgggaccg uuacagugga aguccaauau gccggaaccg acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacgcug acaccugugg ggcgccucau caccgccaau     1140

cccguuauua ccgaguccac agaaaacagc aagaugaugc uggagcugga cccgccuuuu     1200

ggagauucuu acaucgugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

uccggcagca caaucggaaa ggcuuucgag gccacggugc ggggcgcgaa acgcauggcu     1320

guucucgggg acaccgccug ggauuuugga ucu                                  1353


<210>  2442
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE CDS opt

<400>  2442
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugcug     1080

gcucagaugg ccgucgauau gcagacccuc acgcccgugg ggcgccugau cacagccaau     1140

ccuguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcucga ucccccguuu     1200

ggagacuccu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucuggggg acacggccug ggauuuugga agc                                  1353


<210>  2443
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE CDS opt

<400>  2443
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaagcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaggcug agauggaugg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg acaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggaaacccuu     1020

cacgggacag uuaccgugga gguccaauau gccggaacag auggcccuug uaaagugccc     1080

gcucagaugg ccgucgacau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgaaagcac cgagaacucu aagaugaugc uggaacugga uccgccuuuu     1200

ggagacuccu acaucgugau cggcgucggg gagaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg auacggccug ggacuuugga agc                                  1353


<210>  2444
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE CDS opt

<400>  2444
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcggc ccucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaacagggc uugacuucuc cgaucuguau      660

uaccucacca ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgcuggagc cgacacgggc acaccgcauu ggaauaacaa agaggcucug      780

gucgaguuca aggaugccca cgcgaagcgg cagaccgugg uugugcucgg gagccaggaa      840

ggagccgucc auacagcucu ggccggcgcu cuggaagccg agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgcugcguuu accuucacaa agauucccgc cgagacgcuu     1020

cacgggaccg uuacagugga aguccaauau gcuggaaccg acggcccuug uaaagugccc     1080

gcccagaugg ccgucgauau gcagacacug accccugugg ggcgccucau cacggcuaau     1140

cccguuauua cagagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

agcggcucua caaucggaaa ggccuucgag gcgaccgugc ggggcgcuaa acgcauggcc     1320

guucucgggg acacagccug ggauuuugga agc                                  1353


<210>  2445
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE CDS opt

<400>  2445
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg uguccaccac aguuucuaau auggcggaag ugcgcagcua cugcuaugag      240

gcuaccaucu ccgacaucgc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauucaaccc gagaaucucg aguacagaau caugcugagc      480

guccaugggu cucagcacuc cggaaugauu guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau caccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacauuccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagcgg ugguucucgg gagccaggaa      840

ggagccgugc auaccgcucu ggccggcgcu cuggaagccg agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gucagcuacu cccugugcac cgcugcguuu acauucacga agauccccgc cgagacccuu     1020

cacgggacag ugaccguuga agugcaauau gcuggaacag acggcccuug uaaagucccc     1080

gcccagaugg ccguggauau gcagacccug acgccugucg ggcgccucau uacagcuaau     1140

cccgugauca ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucguuau uggcgugggg gaaaagaaaa ucacacauca cuggcauaga     1260

agcggcucua ccauuggaaa ggccuucgag gcgacagucc ggggcgcuaa acgcauggcc     1320

gugcucgggg acacggccug ggauuuugga agc                                  1353


<210>  2446
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE CDS opt

<400>  2446
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucagca agauucccgc ggagacgcuu     1020

cacgggaccg uuacagaaga ggugcaauau gccggaaccg acggcccuug uaaagucccc     1080

gcucagaugg ccguggauau gcagacacug accccugucg ggcgccucau cacggccaau     1140

cccgugauua cagaaucuac cgagaacucc aagaugaugc uggaacugga cccgccuuuu     1200

ggagauagcu acaucguuau cggcgugggg gagaagaaaa uuacccauca cuggcauaga     1260

ucuggcagca caaucggaaa ggcuuucgag gccaccgucc ggggcgcgaa acgcauggcu     1320

gugcucgggg acacagccug ggauuuugga ucc                                  1353


<210>  2447
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE CDS opt

<400>  2447
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga acugcaauau gccggaacag acggcccuug uaaagucccc     1080

gcucagaugg ccguggauau gcagacccuc acgccugucg ggcgccugau cacagccaau     1140

cccgugauua ccgagagcac cgaaaacucu aagaugaugc uggagcucga cccgccuuuu     1200

ggagauuccu acaucguuau cggcgugggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggcuuucgag gccacagucc ggggcgcgaa acgcauggcu     1320

gugcuggggg acacggccug ggauuuugga agc                                  1353


<210>  2448
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE CDS opt

<400>  2448
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggca uuucuaaccg ggacuucguu gaggggauga gcggaggcac cuggguggau      120

gugguccuug aacacggggg augugugaca guuauggccc aagacaaacc uacgguggau      180

aucgagcugg ucaccacaac cguguccaau auggcggaag uucgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugugugc aaacggacac uggucgaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauucaaccc gagaaucucg aguacagaau caugcugagc      480

gugcaugggu cucagcacuc cggaaugauu gucaacgaua ccggccauga aacagacgag      540

aaucgggcua agguugaaau caccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcauuc ugggccugga uugcgagccu agaaccgggc uugacuucuc ugaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

guggaguuca aggaugcuca cgccaagcgg cagacagucg ugguucucgg gucccaggaa      840

ggagcggugc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucagcu ccggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gucucuuaca gccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag ugaccguuga agugcaauau gccggaacag acggcccuug uaaagucccc     1080

gcucagaugg ccguggauau gcagacccug acgccugucg ggcgccucau cacagccaau     1140

cccgugauca ccgaguccac cgaaaacagc aagaugaugc uggagcugga cccgccuuuu     1200

ggagauucuu acauuguuau cggcgugggg gaaaagaaaa uuacacauca cuggcauaga     1260

uccggcagca ccaucggaaa ggcuuucgag gccacagucc ggggcgcgaa acgcauggcu     1320

gugcucgggg acacggccug ggauuuugga ucu                                  1353


<210>  2449
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE CDS opt

<400>  2449
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggugaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

guuagcuacu cccugugcac cgccgccuuu acauucacga agauucccgc ugagacccuu     1020

cacgggacag ugaccgucga agugcaauau gcgggaacag acggcccuug uaaagucccc     1080

gcccagaugg cuguggauau gcagacccug acgccuguug ggcgccucau cacagccaau     1140

cccgugauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgucau cggcgugggg gaaaagaaaa uuacacauca cuggcauaga     1260

agcggcucua ccaucggaaa ggccuucgag gcuacaguuc ggggcgccaa acgcauggcg     1320

gugcucgggg acacggcuug ggauuuugga agc                                  1353


<210>  2450
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE CDS opt

<400>  2450
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggagcug acggcccuug uaaagugccc     1080

gcccagaugg ccgucgauau gcagacacug accccugugg ggcgccucau cacggcuaau     1140

cccguuauua cagagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

agcggcucua caaucggaaa ggccuucgag gcgaccgugc ggggcgcuaa acgcauggcc     1320

guucucgggg acacagccug ggauuuugga agc                                  1353


<210>  2451
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE CDS opt

<400>  2451
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acacaggagc cgacaccggc acaccgcauu ggaauaacaa agaggcucug      780

gucgaguuca aggaugccca cgcuaagcgg cagaccgugg uugugcucgg gagccaggaa      840

ggagccgucc auaccgcgcu ggccggcgcu cuggaagccg agauggacgg ggcuaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac agccgccuuu acguucacca agauucccgc ugagacacuu     1020

cacgggaccg uuacagugga aguccaauau gcgggaaccg acggcccuug uaaagugccc     1080

gcccagaugg cugucgauau gcagacgcug acaccugugg ggcgccucau caccgccaau     1140

cccguuauua ccgagggaac agaaaacagc aagaugaugc uggagcugga cccgccuuuu     1200

ggcgauucuu acaucgugau cggggucggc gaaaagaaaa uuacccauca cuggcauaga     1260

uccggaagca caaucggcaa ggccuucgag gcuacggugc gcggggccaa acggauggcg     1320

guucucggag acaccgcuug ggauuuuggc ucu                                  1353


<210>  2452
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE CDS opt

<400>  2452
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gccauggcgc aagacaaacc uacgguggau      180

auugagcugg ucaccacaac cguguccaau auggcugaag uucgcucuua cugcuaugag      240

gccagcaucu ccgacauggc cucugauagc agauguccga cacagggcga ggcuuaccuc      300

gacaagcagu ccgauaccca guaugugugc aaacggacac uggucgaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgccaaauu cgcuuguucu      420

aagaagauga cgggcaaauc cauccaaccc gaaaaucucg aguacagaau uaugcugagc      480

gugcaugggu cucagcacuc cggaaugcug gucaacgaua ccggccauga aacagacgag      540

aaucgggcca agguugaaau caccccuaac agcccccgcg cggaggccac acucggggga      600

uuuggcucuc ugggccuuga uugcgagccu agaaccgggc uggacuucuc cgaucucuau      660

uaccugacga ugaauaacaa acacuggcuu gcucauaagg aaugguuuca cgacauuccc      720

cugccuuggc acgccggagc ugccacaggc accccgcauu ggaauaacaa agaggcgcuc      780

guggaguuca aggacgccca cgcuaagcgg cagacagugg ucgugcuggg gagccaggaa      840

ggagccguuc auaccgcucu ggccggcgcc cucgaagcug agauggaugg ggcgaaagga      900

cgccuguccu cuggccaccu uaaguguaga cugaaaaugg acaagcuccg gcugaaaggc      960

gugagcuacu cccuuugcac cgccgcuuuu acauucacga agauccccgc cgagaccguc     1020

gaugggacag ugaccguuga aggacaauau ggcgggacag acggaccuug uaaagugccc     1080

gcccagaugg cugucgauau gcagacccug acgccugugg gccgccucau uacagccaau     1140

cccgucauca ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggggauuccu acauugugau cggcguugga gaaaagaaaa ucacacacca uuggcacaga     1260

agcggcucua ccauugggaa ggcguucgag gcuacagugc ggggagccaa acgcauggcc     1320

guccucggcg acacggcuug ggauuuuggg agc                                  1353


<210>  2453
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE CDS opt

<400>  2453
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc ugcggucgau      180

auugagcugg ugacgaccac aguuuccaau auggcugaag ugcgcucuua cugcuaugag      240

gccagcaucu ccgacauggc cucugauagc agauguccga cccagggcga ggcuuaccuc      300

gacaagcagu ccgauacaca guaugucugc aaacggaccc ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugacau gcgccaaauu cgcuuguucu      420

aagaagauga ccggcaaauc cauccaaccc gaaaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua cgggccauga aaccgacgag      540

aaucgggcca agguggaaau uacaccuaac agcccccgcg cggaggccac ccucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaacagggc uugacuucuc cgaucuguau      660

uaccucacca ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgcuggagc cgacacgggc acaccgcauu ggaauaacaa agaggcucug      780

gucgaguuca aggaugccca cgcgaagcgg cagaccgugg uugugcucgg gagccaggaa      840

ggagccgucc auacagcucu ggccggcgcu cuggaagccg agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgcugcguuu accuucacaa agauucccgc cgagacgcuu     1020

cacgggaccg uuacagugga aguccaauau gcuggaaccg acggcccuug uaaagugccc     1080

gcccagaugg ccgucgauau gcagacacug accccugugg ggcgccucau cacggcuaau     1140

cccguuauua cagagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

agcggcucua caaucggaaa ggccuucgag gcgaccgugc ggggcgcuaa acgcauggcc     1320

guucucgggg acacagccug ggauuuugga agc                                  1353


<210>  2454
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE CDS opt

<400>  2454
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuaacaaucg ggacuucgug gaggggaugu cuggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuagcaac auggcggaag ugcgcuccua cugcuaugag      240

gcuucuauca gcgacauggc cuccgauucu agauguccga cacagggcga agccuaccuc      300

gacaagcaga gcgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aauggcugug gccuuuuugg gaagggaucc cuggugaccu gcgcuaaauu cgccuguagc      420

aagaagauga cgggcaaauc uauccaaccc gagaaccucg aguacagaau uaugcugucc      480

guccauggga gccagcacuc uggaaugauc guuaaugaua ccggccauga aacagacgag      540

aaccgggcua agguggaaau uaccccuaau agcccccgcg ccgaggcgac acucggggga      600

uuuggcuccc ugggccugga uugcgagccu agaaccgggc uugacuucuc ugaucuguau      660

uaccucacga ugaacaauaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaacaauaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gucccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucagcu ccggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugucuuaca gccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaac     1140

cccguuauua ccgaguccac cgaaaauagc aagaugaugc uggagcugga cccgccuuuu     1200

ggagauucuu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcauaga     1260

uccggcagca ccaucggaaa ggcuuucgag gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg acacggccug ggauuuugga ucu                                  1353


<210>  2455
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE CDS opt

<400>  2455
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcgc cgcugcguuu accuucacaa agauucccgc cgagacgcuu     1020

cacgggaccg uuacagugga aguccaauau gcuggaaccg acggcccuug uaaagugccc     1080

gcccagaugg ccgucgauau gcagacacug accccugugg ggcgccucau cacggcuaau     1140

cccguuauua cagagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gaaaagaaaa uuacccauca cuggcauaga     1260

agcggcucua caaucggaaa ggccuucgag gcgaccgugc ggggcgcuaa acgcauggcc     1320

guucucgggg acacagccug ggauuuugga agc                                  1353


<210>  2456
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE CDS opt

<400>  2456
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua uuggccauga aaccgacgag      540

aaucgggcua agguggaaau cacaccuaac agcccccgcg ccgaggcgac ccucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaacagggc uugacuucuc cgaucuguau      660

uaccucacca ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacauuccc      720

cuuccuuggc acgccggagc ugacacgggc acaccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagaccgugg uugugcucgg gagccaggaa      840

ggagcggucc auacagcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu accuucacaa agauccccgc ggagacgcuu     1020

cacgggaccg uuacagugga aguccaauau gccggaaccg acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacacug accccugugg ggcgccucau uacggccaau     1140

cccguuauca cagagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau uggcgucggg gacaagaaaa ucacccauca cuggcauaga     1260

agcggcucua caauuggaaa ggcuuucgaa gccaccgugc ggggcgcgaa acgcauggcu     1320

guucucgggg auacagccug ggacuuugga agc                                  1353


<210>  2457
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE CDS opt

<400>  2457
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cgccuguucu      420

aagaagauga cgggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgggcua agguggaaau uaccccuaac agcccccgcg ccgaggcgac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgg cagacagugg uugugcucgg gagccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

cgccucuccu cuggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

gugagcuacu cccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggcgccucau cacagccaau     1140

cccguuauua ccgagagcac cgaaaacucu aagaugaugc uggagcugga cccgccuuuu     1200

ggagauuccu acaucgugau cggcgucggg gacaagaaaa uuacacauca cugguauaga     1260

agcggcucua ccaucggaaa ggcuuucgaa gccacagugc ggggcgcgaa acgcauggcu     1320

guucucgggg auacggccug ggacuuugga agc                                  1353


<210>  2458
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE CDS opt

<400>  2458
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua caggccauga aaccgacgag      540

aaucgggcca agguggaagu gacaccuaac agcccccgcg cugaggccac ccucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaacggggc uugacuucuc cgaucuguau      660

uaccucacaa ugaauaacaa acacuggcug guccauaagg aaugguuuca cgacauuccc      720

cuuccuuggc acgcgggagc cgacaccggc acaccgcauu ggaauaacaa agaggcucug      780

guggaguuca aggaugccca cgcuaagcgg cagaccguug ugguccucgg gagccaggaa      840

ggagccgugc auaccgcgcu ggccggcgcu cuggaagccg agauggacgg ggcuaaagga      900

cgccucuuuu ccggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

guuucuuaca gccugugcac agccgccuuc acguuuacca aggugcccgc ugagacacuu     1020

cacgggaccg ucacagugga aguccaauau gcgggaaccg acggcccuug uaaagugccc     1080

gcccagaugg cuguugauau gcagacgcug acaccugugg ggcgccucau caccgccaau     1140

cccgucauua ccgaguccac agaaaacagc aagaugaugc uggagcugga cccgccuuuc     1200

ggagauucuu acaucgugau uggcguuggg gacaagaaaa ucacccauca cuggcauaga     1260

uccggcagca caaucggaaa ggccuuugaa gcuacggugc ggggcgccaa acgcauggcg     1320

guccucgggg auaccgcuug ggacuucgga ucu                                  1353


<210>  2459
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE CDS opt

<400>  2459
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua uuggccauga aacagacgag      540

aaucgggcca agguggaagu gaccccuaac agcccccgcg cugaggccac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug guccauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgcgggagc cgacacaggc accccgcauu ggaauaacaa agaggcucug      780

guggaguuca aggaugccca cgcuaagcgg cagacaguug ugguccucgg gagccaggaa      840

ggagccgugc auaccgcgcu ggccggcgcu cuggaagccg agauggacgg ggcuaaagga      900

cgccucuuuu ccggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

guuucuuaca gccugugcac cgccgccuuc acauuuacga aggugcccgc ugagacccuu     1020

cacgggacag ucaccgugga aguccaauau gcgggaacag acggcccuug uaaagugccc     1080

gcccagaugg cuguugauau gcagacccug acgccugugg ggcgccucau uacagccaau     1140

cccgucauca ccgaguccac cgaaaacagc aagaugaugc uggagcugga cccgccuuuc     1200

ggagauucuu acauugugau cggcguuggg gacaagaaaa ucacacauca cuggcauaga     1260

uccggcagca ccauuggaaa ggccuuugaa gcuacagugc ggggcgccaa acgcauggcg     1320

guccucgggg auacggcuug ggacuucgga ucu                                  1353


<210>  2460
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE CDS opt

<400>  2460
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua uuggccauga aacagacgag      540

aaucgggcca agguggaagu gaccccuaac agcccccgcg cugaggccac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu agaaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa acacuggcug guccauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgcgggagc cgacacaggc accccgcauu ggaauaacaa agaggcucug      780

guggaguuca aggaugccca cgcuaagcgg cagacaguug ugguccucgg gagccaggaa      840

ggagccgugc auaccgcgcu ggccggcgcu cuggaagccg agauggacgg ggcuaaagga      900

cgccucuuuu ccggccaccu gaaguguaga cuuaaaaugg auaagcugcg gcucaaaggc      960

guuucuuaca gccugugcac cgccgugugu acagccgcua agguccccgc ggagacgcuu     1020

cacgggaccg ugacagucga agugcaauau gccggaaccg acggcccuug caaaguuccc     1080

gcucagaugg ccguggauau gcagacacug accccugucg ggcgccucau uacggccaau     1140

cccgugauca cagaguccac cgaaaacagc aagaugaugc uggagcugga cccgccuuuc     1200

ggagauucuu acauuguuau cggcgugggg gacaagaaaa ucacccauca cuggcauaga     1260

uccggcagca caauuggaaa ggcuuuugaa gccaccgucc ggggcgcgaa acgcauggcu     1320

gugcucgggg auacagccug ggacuucgga ucu                                  1353


<210>  2461
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE CDS opt

<400>  2461
agccagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauuaga       60

ugcaucggcg uuucuaaccg ggacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugcgcucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc agauguccga cacagggcga agccuaccuc      300

gacaagcagu ccgauaccca guaugucugc aaacggacac ugguggaccg cgggugggga      360

aacggcugug gccuuuuugg gaagggaagc cuggugaccu gcgcuaaauu cacguguucu      420

aagaagauga ccggcaaauc cauccaaccc gagaaucucg aguacagaau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaug aaaaucgggc caagguggag      540

gugacaccua acagcccccg cgcugaagcc acccugggcg gguuuggauc ucucggccug      600

gacugcgagc cuagaacagg ccuugauuuc uccgaccugu auuaccucac caugaauaac      660

aaacauuggc ugguccacaa ggagugguuu caugauauuc cccuuccuug gcacagcggg      720

gcggacacgg aaacaccgca cuggaauaac aaagaggccc ugguggaguu caaggacgcu      780

caugccaagc ggcagaccgu ugugguccuc ggaucccagg aaggcgcugu gcacacagcc      840

cuggcggggg cccuggaagc ugagauggau ggagccaaag gccgccucuc uagcgggcau      900

cugaagugua gacuuaaaau ggacaagcug cggcucaaag gaguuuccua cagccugugc      960

accgcugccu uuaccuucac aaaggugccc gccgagacgc uucacggcac cgucacagug     1020

gaaguccaau augcuggccg cgaugggccu uguaaagugc ccgcgcagau ggccguugac     1080

augcagaccc ugacaccugu gggaagacuc aucaccgcua aucccgucau uacggagucu     1140

acagaaaacu ccaagaugau gcuggagcug gauccgccuu uuggcgacag cuacaucgug     1200

auugggguug gagauaagaa aaucacccac cauuggcacc ggucuggcag caucauuggg     1260

aaggccuucg aagccaccgu gcgcggcgcu aaaagaaugg ccguccucgg agacacagcg     1320

ugggauuuug gcucc                                                      1335


<210>  2462
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE CDS opt

<400>  2462
agccagaagg ugaucuaccu ggucaugauu guucucaucg ugcccgccua uuccauuucu       60

ugcaucggcg ugagcaacag agaccugguc gaggggaugu ccggaggcac cuggguggau      120

guugugcuug aacacggggg augugucaca gagauggcuc aagacaaacc uacgguggau      180

auugaacugg uuaccaugac agugucuaau auggccgagg uccggagcua cugcuaugaa      240

gcgucccucu cugacauggc uagcgccucc cgcuguccga cccagggcga gcccagccug      300

gauaagcagu cugacacaca guccgugugc aaaagaaccc uuggggaucg gggauggggc      360

aacggcugug ggaucuucgg aaagggcagc cuggugacau gcucuaaauu uaccuguugc      420

aagaagaugc cugggaaauc cauccaaccc gagaaucucg aauaccgcau uaugcugccu      480

guccauggaa gccagcacuc uggcaugauc guuaacgaca uugggcauga gacggaugaa      540

aauagagcca agguggaggu gacccccaac uccccucggg cugaggccac acugggaggc      600

uucggcagcc ucgggcugga cugugaaccg cgcaccggac uugauuuuuc cgaccuguau      660

uaccucacaa ugaauaacaa acacuggcug guccauaagg agugguucca cgauaucccc      720

cuuccuuggc acgcuggcgc cgacaccggg acgccccauu ggaauaacaa agaagcgcug      780

guggaguuua aggacgccca cgcuaagaga cagacaguug ugguccucgg aucucaggaa      840

ggcgccgugc auaccgcucu ggccggggcg cuggaggccg agauggaugg agcuaaaggc      900

cgccucuuca gcggccaccu gaagugccgg cuuaaaaugg acaagcugag acucaaaggg      960

guuuccuaua gccuguguac agccgcuuuu accuucacca aggugccugc cgaaacacuu     1020

cacggaacgg ucaccgugga gguccaaucu gccggcacag augggcccug caaagugccg     1080

gcucagaugg cgguugacau gcagacccug acaccugugg gacggcucau uaccgccaau     1140

cccgucauca cggaauccac agagaacagc aagaugaugc uggaacugga uccucccuuu     1200

ggcgacucuu acauugugau cgggguuggc gauaagaaaa ucacccauca cuggcaucgc     1260

agcggaucca ccauuggcaa ggcuuucgag gccacaguga gaggggccaa acggauggcu     1320

guccucggag acaccgccug ggauuuuggc ucu                                  1353


<210>  2463
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE CDS opt

<400>  2463
agacagaagg ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uagcauucgg       60

ugcaucggcg uuuccaaccg cgacuucgug gaggggaugu cuggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaaacc uacggucgau      180

auugagcugg ugaccacaac cguuagcaau auggcggaag ugagauccua cugcuaugag      240

gcuucuauca gcgacauggc cuccgauucu cgguguccga cacagggcga agccuaccuc      300

gacaagcaga gcgauaccca guaugucugc aaacgcacac ugguggacag agggugggga      360

aacggcugug gccuuuuugg gaagggaucc cuggugaccu gcgcuaaauu cgccuguagc      420

aagaagauga cgggcaaauc uauccaaccc gagaaucucg aguaccggau uaugcugucc      480

guccauggga gccagcacuc uggaaugauc guuaacgaua ccggccauga aacagacgag      540

aaucgcgcua agguggaaau uaccccuaac ucccccagag ccgaggcgac acucggggga      600

uuuggcagcc ugggccugga uugcgagccu cggaccgggc uugacuucuc ugaucuguau      660

uaccucacga ugaauaacaa acacuggcug gugcauaagg aaugguuuca cgacaucccc      720

cuuccuuggc acgccggagc ugacacaggc accccgcauu ggaauaacaa agaggcccug      780

gucgaguuca aggaugcuca cgccaagcgc cagacagugg uugugcucgg gucccaggaa      840

ggagcggucc auaccgcccu ggcuggcgcc cuggaagcug agauggacgg ggccaaagga      900

agacucagcu ccggccaccu gaagugucgg cuuaaaaugg auaagcugcg ccucaaaggc      960

gugucuuaca gccugugcac cgccgcuuuu acauucacga agauucccgc ggagacccuu     1020

cacgggacag uuaccgugga aguccaauau gccggaacag acggcccuug uaaagugccc     1080

gcucagaugg ccgucgauau gcagacccug acgccugugg ggagacucau cacagccaau     1140

cccguuauua ccgaguccac cgaaaacagc aagaugaugc uggagcugga cccgccuuuu     1200

ggagauucuu acaucgugau cggcgucggg gaaaagaaaa uuacacauca cuggcaucgg     1260

uccggcagca ccaucggaaa ggcuuucgag gccacagugc gcggcgcgaa aagaauggcu     1320

guucucgggg acacggccug ggauuuugga ucu                                  1353


<210>  2464
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE CDS opt

<400>  2464
agccagagag ugaucuaccu ggucaugauu cuccugaucg cccccgcuua uuccauucgg       60

ugcaucggcg uuucuaaccg cgacuucgug gaggggauga gcggaggcac cuggguggau      120

gucgugcuug aacacggggg auguguuaca gugauggccc aagacaagcc uacggucgau      180

auugagcugg ugaccacaac cguuuccaau auggcggaag ugagaucuua cugcuaugag      240

gcuagcaucu ccgacauggc cucugauagc cgguguccga cacagggcga agccuaccuc      300

gacaaacagu ccgauaccca guaugucugc aagcgcacac ugguggacag agggugggga      360

aacggcugug gccuuuuugg gaaaggaagc cuggugaccu gcgcuaaguu cacguguucu      420

aaaaagauga ccggcaaguc cauccaaccc gagaaucucg aguaccggau uaugcugagc      480

guccaugggu cucagcacuc cggaaugauc guuaacgaua uuggccauga aacagacgag      540

aaucgcgcca aaguggaagu gaccccuaac agccccagag cugaggccac acucggggga      600

uuuggcucuc ugggccugga uugcgagccu cggaccgggc uugacuucuc cgaucuguau      660

uaccucacga ugaauaacaa gcacuggcug guccauaaag aaugguuuca cgacaucccc      720

cuuccuuggc acgcgggagc cgacacaggc accccgcauu ggaauaacaa ggaggcucug      780

guggaguuca aagaugccca cgcuaagcgc cagacaguug ugguccucgg gagccaggaa      840

ggagccgugc auaccgcgcu ggccggcgcu cuggaagccg agauggacgg ggcuaaggga      900

agacucuuuu ccggccaccu gaaaugucgg auuaagaugg auaaacuucg ccugaagggc      960

guuucuuaca gccucugcac cgccgccuuc acauuuucca aagugcccgc ugagacgcug     1020

cacgggaccg ucacagugga aguccaauau gcgggaagag acggcccuug uaaggugccc     1080

gcccagaugg cuguugauau gcagacccuu acaccugugg ggcggcugau caccgccaau     1140

cccauuauca cggagagcac agaaaacucu cgcaugaugc ucgagcugga cccgccuuuc     1200

ggagauuccu acaucgucau uggcgugggg gacaaaaaga ucacccauca cuggcauaga     1260

agcggcucua ccauuggaaa ggccuuugaa gcuacaguuc ggggcgccaa aagaauggcg     1320

gugcuggggg auaccgcuug ggacuucgga agc                                  1353


<210>  2465
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  2465
aggauaauaa augccagaaa ggaaaaaaaa agaagaggag cugauacaag uguaggaaua       60

guaggauuau uauugacaac agcaauggca gcugaaguaa cgagaagagg uucagcauau      120

uauauguacu uagacaggaa ugaugcaggu gaagcaauau cauucccaac aacacuagga      180

augaauaaau guuauauaca aauuauggac cucggacaua ugugugaugc uacgauguca      240

uaugaaugcc cuaugcugga cgagggaguu gaaccagaug auguugauug uuggugcaac      300

acaacaagua caugggucgu auauggaaca ugucaucaua aaaaaggaga agcacgaagg      360

aguagaagag cuguaacauu accaucacau ucaacgcgaa agcuacaaac gcgaucacaa      420

acaugguuag aaagcagaga auacaccaaa caucuaauua gaguagagaa uuggauuuuu      480

agaaauccag gauuugcacu ggcggccgcu gcaauugcau ggcuauuagg aaguucaacc      540

agucaaaaag uaauauaccu aguaaugaua uuacuaauag cgccagcuua uagcauaaga      600

ugcauaggag uuaguaaccg agauuuuguc gagggaaugu caggugguac uuggguagac      660

auagugcuag aacauggugg auguguaaca guuauggcac aggacaaacc aacaguagac      720

auugaacucg uaacaacaac uguaaguaau auggcagaag uacggucaua cuguuaugaa      780

gcuaguauau cagauauggc aagcgacagc agaugcccaa cucaagggga agcauacuua      840

gauaaacaaa gugauacuca guauguaugu aaacgaacau uaguagacag aggaugggga      900

aacggaugug gacuuuucgg uaaagguagc cuagugacau gugcaaaauu ugcuuguagu      960

aaaaaaauga cuggaaaauc aauacaaccc gaaaaucuag aauauagaau aauguuguca     1020

guccacggau cacagcauuc ugguaugauu guaaaugaua cuggacauga aacagaugaa     1080

aauagagcaa aaguagaaau aacaccaaac aguccaaggg cagaagcaac auuagguggu     1140

uuuggaucac uaggguuaga uugugaacca aggacgggau uagauuuuag cgacuuauac     1200

uauuuaacca ugaacaauaa acacugguua guacacaaag aaugguuuca cgauauaccg     1260

uugccguggc augcgggagc ugauacaggc acaccacauu ggaauaauaa agaagcccua     1320

guagaauuca aagacgcaca cgcgaaaaga caaacaguag uaguauuagg uucgcaagaa     1380

ggcgcagugc auaccgcauu ggcaggugca uuggaagcag aaauggaugg agcaaaagga     1440

agguuaaguu cuggacauuu gaaauguaga uugaaaaugg auaaacuaag acuaaaagga     1500

gugagcuauu cauuauguac cgcagcauuu acauucacaa aaauuccagc agaaacguua     1560

cacggaacag uuacgguaga aguacaauau gcagggacag auggccccug uaaaguacca     1620

gcucaaaugg cgguugauau gcaaacccua accccagugg gaagauuaau uacagcuaac     1680

ccaguaauaa cagaaaguac cgaaaauagc aaaaugaugc uugaacuaga cccaccauuu     1740

ggagauucau acauaguaau aggaguugga gaaaaaaaga uaacacauca uuggcauagg     1800

aguggaagua cuaucggaaa agcguuugaa gcgacaguua gaggagcaaa aagaauggcu     1860

guguuagggg auacagcaug ggauuuugga agcgucggag gagccuuaaa uucuuuagga     1920

aaaggaauuc aucaaauauu uggggccgca uucaaaaguc uauuuggugg aaugucaugg     1980

uuuucacaaa uauuaauagg aacauuguua auguggcuag gauuaaacac uaaaaacgga     2040

agcauaucac uaaugugucu agcuuugggu ggaguacuaa uauuucuauc cacugcugua     2100

ucagcagacg ugggguguag cguggacuuc aguaaa                               2136


<210>  2466
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS opt

<400>  2466
agaauaauca augcgaggaa agaaaagaaa agaagaggag ccgacacuag cgucgguaua       60

guagguuuau uauuaacaac ugccauggcc gccgaaguua caagaagagg uucagcauac      120

uacauguauu uagauagaaa ugaugcagga gaagcuauuu ccuuuccuac aacucuugga      180

augaauaaau guuauaucca aauuauggau cuaggacaua cgugcgaugc aacaaugagc      240

uacgaauguc caauguuaga ugaaggagua gaaccagaug acguugauug cugguguaac      300

acaacuucaa cauggguagu uuauggcaca ugucaucaua agaaaggaga agcaagaaga      360

aguagacgug caguaacauu accaagucau ucaacuagaa aacuacaaac uaggagccaa      420

acauggcuug aaaguagaga guauacaaaa caccuuauua gaguagaaaa uuggauauuu      480

aggaauccag gauucgcguu agcagcagcu gcaauagcau gguugcuagg aagcucaaca      540

agucaaaaag uaauauauuu aguaaugaua cuucuaauag caccagcaua uucaauaaga      600

uguaucggag uaucaaaccg agauuuuguc gaaggaaugu cuggugguac auggguagac      660

guaguauuag agcacggagg auguguaaca guaauggcac aagauaaacc aacaguggau      720

auagaauugg ucacaacaac cguaucaaau auggcugaag ucagaagcua cugcuaugaa      780

gcaucgauaa gugauauggc gagugacucu agaugcccaa cccaaggaga agcauacuua      840

gauaaacaau cagacacaca auacguaugu aaaagaacuu uaguagauag agguuggggc      900

aacggaugug gacucuuugg aaaaggaucg cuaguuacau gugcaaaauu ugccuguuca      960

aagaaaauga cgggaaaauc aauacagcca gaaaauuuag aauauagaau aauguugagu     1020

guacauggau cccaacauuc aggaaugaua guaaaugaua caggacauga aacugacgaa     1080

aauagagcaa aaguugaaau aacaccaaau ucaccacgug cagaggcgac acucgguggu     1140

uucgggucau ugggucuuga uugugaacca agaacaggau uagauuuuuc agauuuauau     1200

uacuuaacaa ugaacaauaa acacugguua guacauaaag aaugguuuca ugauauaccg     1260

uuaccauggc augcaggagc agauacaggc acaccacauu ggaauaauaa agaagcauua     1320

guugaguuca aagaugcgca cgcuaaaaga caaacaguug ucguauuagg uagucaagaa     1380

ggagcaguac auacagcgcu agcuggagca cuagaagcug aaauggaugg agcaaaagga     1440

agacuaagua guggucauuu aaaauguaga uuaaaaaugg acaaacuuag guuaaaagga     1500

guuaguuacu cauuauguac ugcggcuuuu acauuuacaa aaauaccugc agaaacauua     1560

cacgguacag uuacagucga aguacaauau gcugguacag acggaccuug uaaaguacca     1620

gcucaaaugg caguagauau gcaaacacua acaccaguug gacgccuaau aaccgcaaau     1680

ccuguuauaa cagaaucuac ggaaaacuca aaaaugaugu uagaauuaga ucccccauuu     1740

ggggauucau auauaguaau ugggguagga gaaaagaaaa uaacacauca cuggcacaga     1800

aguggaagua caauaggaaa agcauucgaa gcaacaguaa gaggagcaaa aagaauggca     1860

guccuagggg acacugcaug ggauuucgga agcguaggag gggcuuuaaa uagucucggg     1920

aaaggaaucc aucaaaucuu uggagcagcg uuuaaaucac uauuuggagg aaugucaugg     1980

uuuucacaaa uauuaauagg uacuuuacua augugguuag gacuaaaugc uaaaaacggc     2040

ucaauuucau uaauguguuu agcauuagga ggaguuuuga uauucuuauc aacagcaguu     2100

agcgcugacg uuggauguag uguagauuuu agcaaa                               2136


<210>  2467
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS opt

<400>  2467
cgaauaauaa acgcgagaaa agaacguaaa cgucgaggag cagacacaag cauaggcaua       60

auaggauuac ugcuaacaac cgcaauggca gccgaaauaa caagaagagg aucagccuau      120

uauauguauc uagauagaag ugaugcaggu aaagccauau cauuugcaac cacguuagga      180

guaaauaaau gccauguaca aauaauggac cuuggacaua ugugcgaugc aacaaugucu      240

uaugaauguc cuaugcuaga ugaaggagua gaaccagaug acguugauug uugguguaau      300

acaacuucca cauggguagu cuacggaaca ugccaucaca aaaaagguga agcuaggagg      360

ucaagacgag ccguaacauu accaucucau ucaacaagaa aauuacaaac aagaucacaa      420

acauggcuag aaucaaggga auauacaaaa caucuuauaa aaguagaaaa uuggauuuuc      480

agaaauccug guuuugcacu aguagcagua gcaauagccu ggcucuuagg uagcaguacc      540

agccaaaaag uaauauaucu cgucaugauu cuauuaauag cuccagccua cagcauaaga      600

uguauuggag uaucaaauag agauuuugua gaagguaugu cuggaggaac auggguugau      660

guaguuuuag aacauggggg auguguaaca guaauggcac aagauaaacc aacaguggac      720

auugaauuag ucaccacaac uguaucuaau auggcagagg uacgaucaua uuguuaugaa      780

gcaagcauaa gcgauauggc gagugauuca agaugcccca cacaaggaga agcuuauuua      840

gauaaacaaa gugauacaca guauguaugc aaaagaaccc uaguggauag agguuggggu      900

aaugguugcg gauuauuugg aaaaggaagu cuaguaacau gugcaaaauu cacuuguuca      960

aaaaaaauga cagggaaaag uauacaacca gaaaauuuag aauauagaau aauguuaagc     1020

guacaugguu cacaacauuc gggaaugaua ggauaugaaa cagaugaaga uagggccaaa     1080

guagaaguaa caccaaacag uccaagagca gaagccacac uuggugguuu cggaucucuc     1140

ggauuagacu gugaaccucg aacagggcuu gacuuuucag aucuauacua uuuaacaaug     1200

aauaauaagc auuggcuagu ucacaaagaa ugguuucacg auauuccgcu uccauggcau     1260

gcgggagcag auacaggaac uccacauugg aauaauaaag aagcacuagu ugaauucaaa     1320

gaugcacaug caaaaagaca aacagucgua guuuuaggau cacaagaagg agcaguacau     1380

acagcacuag ccggagcuuu agaagcagaa auggauggug caaaaggacg acuauuuagc     1440

ggacaccuaa aaugucgucu aaaaauggau aaacuuagau uaaaaggagu aucguacucg     1500

uuauguacag cagcauuuac auuuacuaaa gugcccgcag aaacacuaca uggaacagug     1560

acuguugaag uacaauaugc aggaacagau ggcccaugua aaauuccugu ucaaauggca     1620

guugauaugc aaacauuaac uccaguugga agacuaauaa cagcaaaccc uguuauaaca     1680

gaaucaacug aaaauaguaa aaugauguug gaacuggacc caccauucgg agauaguuac     1740

auaguaauag guguaggaga uaagaaaaua acacaucauu ggcacagauc agguaguacc     1800

auugguaaag cauucgaagc aacaguuaga ggagcaaaaa gaauggcagu ucucggagau     1860

acagcauggg auuuugguag ugugggaggu guuuuuaaca guuuagguaa aggaauacau     1920

caaaucuuug gugcagcuuu uaaaagccua uuuggaggaa ugucaugguu cucucaaauu     1980

cuaauaggua cacuauuagu uuggcuagga cuuaauacga aaaauggguc uauuaguuua     2040

acuuguuuag cacucggagg aguaaugaua uucuuaagua cagcaguauc cgcugacguc     2100

gguuguucag uagauuucuc aaaa                                            2124


<210>  2468
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) CDS opt

<400>  2468
agaauaauaa augcaaggaa agaaaagaaa agaagaggag cagacacaag cguagguaua       60

guaggguuau uauuaacaac ugcuauggcu gcugaaguaa caaggagggg uagugcauau      120

uauauguauu uagacagaaa cgaugcaggg gaagcaauaa guuuuccuac aacauuagga      180

augaauaaau guuauaucca aauuauggac uuaggccaua ugugugacgc uacaaugagu      240

uacgaauguc caauguuaga ugaaggugua gaaccagacg auguggauug uugguguaac      300

acaacaucaa cauggguagu auaugguaca ugucaucaua agaaaggaga agcaagaaga      360

ucaaggagag cuguaacucu accuucacac agcacaagaa aauuacaaac uagaucacag      420

accugguuag aaucuagaga auauacaaaa cauuuaauca gaguagaaaa uuggaucuuc      480

agaaaccccg gguuugcucu cgcagcagca gcaauagcau gguuacuugg aaguucuaca      540

ucacaaaaag uuauuuaccu aguaaugauu cuacuaauag caccagcuua uagcauaaga      600

ugcauuggag uuaguaacag agacuuugua gaaggaaugu caggugguac uugggucgau      660

gugguauuag aacauggggg augcguaacu guuauggcac aagacaaacc aacaguagau      720

aucgagcuag uaacaacaac gguaagcaac auggcagaag ugagaaguua uuguuaugaa      780

gcaagcauaa gugauauggc auccgauuca cgauguccaa cacaagguga agcauaccua      840

gauaaacaau cagauacaca auauguaugu aaaagaacau uaguugauag ggguugggga      900

aauggaugug gacuauucgg aaaaggguca uuagugacgu gugcaaaauu ugccugcagu      960

aagaaaauga cugggaaaag cauucaacca gaaaaucuag aauauagaau aaugcuaagu     1020

guacauggau cucaacauag uggcaugaua guaaacgaua caggacacga aacugacgaa     1080

aacagagcua aaguagaaau cacucccaau agcccaagag cugaagcaac acuaggagga     1140

uuuggaucau uaggacuuga cugugaaccu agaacaggau uggacuuuag cgaucuauac     1200

uaucuaacaa ugaauaauaa acauuggcua guacauaaag aaugguuuca ugauauucca     1260

cuuccauggc acgccggcgc agacacaggg accccucacu ggaacaauaa agaagcauua     1320

guagaguuca aagaugcuca ugcaaaaaga caaacaguag uuguauuagg uucgcaagaa     1380

ggagccguac auacugcacu ugcaggagcu uuagaagcag aaauggaugg agcaaaagga     1440

cgauuaucua guggacaucu aaaauguaga cuaaaaaugg acaaauuaag auuaaaagga     1500

guaagcuauu cauuauguac agcagcauuu acauucacaa aaauaccugc ugaaacccua     1560

cacggcacag uaacaguaga aguucaauau gcaggaacug auggaccuug caaaguaccc     1620

gcacaaaugg caguugauau gcaaaccuua accccuguug gcagacuaau cacagcaaac     1680

ccaguuauaa cagaaucaac agaaaauagc aaaaugaugc uagaacugga uccaccuuuc     1740

ggugauucau auauaguaau aggaguaggg gaaaagaaaa uuacucauca cuggcauaga     1800

ucaggaucaa caaucgguaa agcauucgaa gcuacaguac gaggggcaaa aagaauggca     1860

guacuaggag auacugcaug ggauuuuggu aguguaggug gagcacuaaa uuccuuggga     1920

aaaggaauac accaaauauu uggagcugca uuuaaaaguc uauuuggagg aaugagcugg     1980

uuuagccaga uucuaauugg aacauuacuu auguggcucg gauuaaacac aaagaaugga     2040

aguauuucau uaauguguuu agcucucggu ggcguauuaa uauuuuuaag uacagcaguu     2100

agcgcagacg uuggauguuc aguagauuuu aguaaa                               2136


<210>  2469
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  2469
acaagcguag gaauuguugg acuauugcua acaacugcaa uggcugcaga gguaacucga       60

agaggcagcg cauacuacau guauuuggau agaaaugacg caggggaagc aauaagcuuu      120

ccaacgacau uaggaaugaa uaaauguuau auacaaauaa uggauuuagg acacaugugu      180

gaugcaacua ugaguuauga gugcccaaug cuugaugaag gagucgaacc agaugauguu      240

gauuguuggu guaauaccac aaguacaugg guugucuacg gaacauguca ccauaaaaaa      300

ggugaagcua gaagaaguag aagagcagua acacuaccca gucacucaac uagaaaacua      360

caaaccagau cccaaacaug guuagaaagu agggaauaca caaaacaucu aauuagaguu      420

gaaaacugga uauuuagaaa uccagguuuc gcuuuagcag cagcagcaau ugcuugguua      480

uuaggaaguu caacaucaca gaaaguuaua uaccuaguaa ugauacuacu aauugcaccc      540

gcauauagua uacgauguau uggaguuagc aaucgugauu ucguugaagg aaugucagga      600

ggaacuuggg uugacauagu auuagaacau ggagggugug uaacgguuau ggcacaagau      660

aaaccaacgg uugauauaga gcuaguaaca acaacaguua gcaauauggc agaaguacga      720

ucauauugcu augaagcuuc aauaucagau auggcaucag auucgagaug uccaacacaa      780

ggggaagcau auuuagauaa acaaagugau acacaauaug uauguaaacg aacacuagua      840

gauagagguu gggggaaugg augugguuua uucggaaaag gaucacuugu aacaugugca      900

aaguuugcau guucaaaaaa aaugacaggc aaaagcauac aaccagaaaa ucuagaauac      960

agaaucaugu uaucaguaca uggaucucaa cauagcggaa ugauaguuaa ugacacagga     1020

caugaaacag augaaaauag agcgaaagua gagauaacac cuaauagucc aagagcagaa     1080

gcaacauugg gaggauucgg aucucuagga cuagauugug aaccacgaac aggacuugac     1140

uuuucugauu uauacuaucu aacaaugaac aauaaacacu ggcuaguaca uaaagaaugg     1200

uuucacgaua uaccacuacc auggcacgcc ggagcagaua ccggaacacc acauuggaau     1260

aauaaagaag cauuaguaga auuuaaagau gcccaugcua aaagacaaac aguuguagug     1320

cuuggaaguc aagaaggagc aguacauaca gcauuggcug gugcauuaga agcagaaaug     1380

gauggugcaa aaggaaggcu aaguucugga caucuaaaau guaggcuaaa aauggauaaa     1440

uuaagauuaa aaggaguauc auauucauua uguaccgcug ccuucacuuu uacaaaaaua     1500

ccagcagaaa cgcugcacgg aacuguaaca guugaaguac aauaugcugg aacagaugga     1560

ccaugcaaag uuccugcaca aauggcugua gacaugcaaa cacugacucc uguugggaga     1620

cuaauuacag caaaccccgu aauaacagaa aguaccgaaa acucuaaaau gauguuagaa     1680

uuagauccac cuuucgggga uaguuauaua guaauaggag uaggggaaaa aaaaauaacu     1740

caucacuggc auaggagcgg caguacuaua gguaaagccu uugaagcaac ugucagaggu     1800

gcaaaaagaa uggcaguguu aggugacaca gcaugggauu ucggaucagu aggaggugca     1860

uuaaauaguu uaggcaaagg cauucaucaa auauuugggg ccgcauucaa aagucuguuc     1920

ggaggaauga gcugguucag ucagauauua auaggaacac uauuaaugug gcuaggucua     1980

aacacaaaaa acgggaguau uagucucaug ugucuugcau uaggaggagu auuaauauuu     2040

uuaaguacag cagucucagc a                                               2061


<210>  2470
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) CDS opt

<400>  2470
accucaguag guauaguagg uuuacuauua acaaccgcaa uggcagcaga agugacaaga       60

agaggaucag cauacuauau guauuuagau agaaaugaug caggagaggc aauuucauuc      120

ccaacaaccu uaggaaugaa caaauguuau auacaaauaa uggauuuagg acauacaugu      180

gacgcaacaa ugaguuacga auguccaaug cuagacgaag guguagaacc agaugacgua      240

gacugcuggu guaauacaac aucaacaugg guuguuuacg gaaccuguca ucauaagaaa      300

ggugaagcaa gaagaaguag acgagccgua acucuaccau cacacucaac cagaaaacuc      360

caaacaagaa gucagacaug gcuugaaagc cgagaauaca cuaaacauuu aauaagaguu      420

gaaaauugga uuuuuagaaa cccaggauuc gcauuagcag cagcagcaau agcgugguua      480

cuagguucau caacgucaca aaaaguuauu uauuuaguca ugauucuguu aauagcacca      540

gcuuacagua uaagauguau cggaguauca aauagggacu uuguugaagg aauguccgga      600

gguacuuggg uagauguagu auuagaacau ggaggaugug uaacaguaau ggcacaagac      660

aaaccaaccg uagauauaga gcuaguaacu acuacaguau ccaauauggc agaaguacgu      720

aguuauuguu augaagccag cauaagugau auggcaucag auucaagaug cccaacacaa      780

ggugaagcau auuuagauaa acaaagugac acacaauacg uauguaaaag aacauuagua      840

gacagaggau ggggaaaugg uuguggauua uucgggaagg gaucacuugu aacuugugcc      900

aaauucgcau gcucaaagaa aaugacaggg aaaucgauac aaccagaaaa uuuagaauac      960

agaauaaugc ucaguguaca uggaagccaa cauucaggaa ugaucguuaa ugauacuggu     1020

caugaaacag acgaaaacag agccaaaguu gaaauaacac cuaauucacc aagagcugaa     1080

gcaacauuag gaggauucgg aucauuaggu cuagacugug aaccaagaac aggucuagac     1140

uuuagcgauu uauauuaccu aacuaugaau aauaaacauu ggcuaguuca uaaagaaugg     1200

uuucaugaua uaccacuccc uuggcaugca ggagcagaca caggaacacc acauuggaau     1260

aauaaagaag ccuuaguaga auuuaaagau gcacaugcua aaagacagac aguuguugua     1320

cucggaucuc aagaaggagc aguacauaca gcauuagcag gagcauuaga agcagaaaug     1380

gauggugcaa aagguagauu aucuaguggu caccuuaaau gucgauuaaa aauggauaaa     1440

uuaagauuaa aagggguuuc auauagucuu ugcacugcug cguuuacauu uacuaaaaua     1500

ccagcagaaa cucuacacgg cacaguaacu guagaaguac aauaugcugg aacugacggc     1560

cccuguaaag uaccagcgca aauggcuguc gauaugcaaa cccuaacacc cguaggaagg     1620

cugauaacag caaauccugu aauaacagaa aguacggaaa auucaaaaau gauguuagaa     1680

cuagauccac cauucggcga uucauacaua guaauuggag uaggagaaaa gaaaauaacc     1740

caccauuggc auaggagcgg aagcacaaua ggaaaagcau ucgaagcaac aguaagaggu     1800

gcaaaaagaa uggcaguacu aggagauaca gcuugggacu uuggauccgu ugguggagca     1860

uuaaauucgu uagguaaagg aauacaccaa auuuucggag cagcuuucaa aucccuauuu     1920

ggaggaaugu caugguuuuc ccaaaucuua auaggaacac uauuaaugug gcuaggccua     1980

aaugcuaaaa auggaucuau aucauuaaug ugcuuagcac uaggcggcgu ucugauauuu     2040

uuaagcacug caguaucagc a                                               2061


<210>  2471
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) CDS opt

<400>  2471
acaaguauag gaaucauugg auuacuacua acaacugcaa uggcagcaga aauaacuaga       60

cgaggaucag cauauuacau guauuuagau agauccgaug cuggcaaagc aauuucauuu      120

gcaacaacac uaggaguaaa caaaugucau guucaaauaa uggaucuagg acauaugugu      180

gaugccacaa ugucguauga guguccaaug uuagaugaag guguagaacc agacgaugua      240

gauugcuggu guaauacgac auccacaugg guaguauaug gaacuuguca ucacaagaaa      300

ggagaggcaa gacgcuccag gagagcaguu acauuaccaa gucauaguac aagaaaauua      360

caaacaagaa gccaaacuug gcuugaaucg agagaauaua caaaacauuu aauuaaagua      420

gaaaauugga uauuuagaaa cccaggauuc gcauuagugg cagucgcaau ugccuggcua      480

uuagggagca guacaucaca aaaaguaaua uaucucguaa ugauacuauu aauagcuccu      540

gcauacagca uaagaugcau aggaguuuca aaccgagauu uuguagaagg uaugagcgga      600

ggcacauggg uagacguugu acuagaacau ggaggaugug ucacugugau ggcacaagac      660

aaaccaacag uagauaucga acugguaaca acaacaguaa guaauauggc agaaguaaga      720

uccuauugcu augaagcauc gauaucagac auggcaagug auucuagaug cccaacucaa      780

ggagaagcau auuuagauaa acaaagcgac acacaauaug uaugcaagag gacucuagua      840

gauagagguu gggguaaugg auguggcuua uuuggaaaag gaagucucgu aacaugcgca      900

aaguucacau guucaaagaa aaugacugga aaaucuauac aaccagaaaa uuuagaguau      960

agaauaaugc ucagcguaca ugguucacaa cacaguggaa ugaucggaua cgaaacugac     1020

gaagaccgag caaaaguuga aguaaccccu aauucaccaa gagcagaagc aacauuagga     1080

ggguucggaa gccucggauu agacugcgaa ccacgaacag gauuagauuu uucagaucua     1140

uacuauuuaa caaugaacaa caaacauugg uuaguucaua aagaaugguu ucaugauaua     1200

ccacuaccau ggcaugcagg cgcugauaca ggcacuccac auuggaauaa caaagaagca     1260

uuaguagaau uuaaagacgc acacgcaaaa cgacaaacag uaguaguuuu aggaagccaa     1320

gaaggagcgg uacacacugc auuagccggu gcauuagaag cagaaaugga uggugcaaaa     1380

ggaagacuau uuucugguca uuuaaaaugu cgauuaaaaa uggauaaauu aagacucaaa     1440

ggaguaucuu acaguuuaug uacagcagca uucacauuca caaaaguacc ugcagaaaca     1500

uuacauggaa cgguuacagu agaagugcaa uaugcuggaa cagauggacc auguaaaaua     1560

ccagugcaaa uggcuguaga uaugcagacu uuaacaccag ucggaagauu aauaaccgca     1620

aauccaguaa uaacagaaag uaccgaaaau ucaaaaauga uguuagaauu agauccacca     1680

uuuggcgaca guuauauugu uauaggagua ggcgauaaga aaauaacaca ucacuggcau     1740

agaagcggau cuacaauagg aaaagccuuu gaagcaacag uaagaggagc caaaagaaug     1800

gcaguacucg gugauacagc uugggauuuc ggaucuguug gaggaguuuu caauucauua     1860

ggcaaaggaa uacaucaaau auucggagcu gccuuuaaau cauuauuugg uggaauguca     1920

ugguuuucuc aaauucuaau agguacauua cuuguauggc uaggacuaaa cacuaaaaau     1980

gguucaauau cacuaacaug ucuagcuuua ggcggaguaa ugaucuucuu aagcacagcu     2040

guaucagca                                                             2049


<210>  2472
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) CDS opt

<400>  2472
acaucaguag gaauagucgg acuuuuguua acgacagcca uggcagcuga aguaacaaga       60

agaggcucug cauacuauau guaucuagac cggaacgacg ccggagaagc aauaucauuc      120

ccaacaacau uaggcaugaa uaaaugcuac auacaaauua uggaucuugg acauaugugc      180

gaugcaacaa ugucuuauga auguccaaug uuggaugaag gaguugaacc ugacgaugua      240

gacugcuggu gcaacacaac aucaacaugg guuguauaug guaccuguca ucauaaaaaa      300

ggcgaagcca gaagaucaag aagggcagua acacugccua gucauaguac uagaaaauua      360

caaacuagau cacaaacuug guuagaauca agggaauaca caaaacaccu aauacgagua      420

gaaaauugga uauuucgaaa uccaggguuc gcacuagcag cagcugcaau agcauggcuu      480

uuaggaaguu caacaagcca aaaagucaua uaucuaguua ugauucuacu uauagcaccu      540

gcauacucaa uacgaugcau aggagucuca aauagggauu uuguagaagg uaugucagga      600

gggaccuggg uagacguagu acuagaacau gguggaugug uaacagucau ggcacaagau      660

aaaccaacag uagacauaga auuaguaaca acgacugucu ccaauauggc cgaaguuaga      720

ucauacugcu acgaagcuuc uauaucagau auggcaagcg auucaagaug ucccacccaa      780

ggugaagcau aucuagacaa acaaucagau acacaauacg uuuguaaaag aacauuagua      840

gacagagguu ggggaaaugg auguggauua uuuggaaaag guaguuuagu aacaugcgca      900

aaauuugcuu gcagcaaaaa aaugacagga aaauccauac aaccagaaaa ccuugaguau      960

agaauaaugc ucaguguuca uggaucacaa cauucuggaa ugauaguaaa ugacacagga     1020

cacgaaacug acgagaaucg ugcuaaagua gaaauaaccc caaauagucc uagagcagaa     1080

gcaacauuag gcggauuugg aaguuuagga uuagauugug aaccaagaac aggcuuggau     1140

uuuagugauc uauauuacuu aacaaugaau aacaaacauu gguuggucca uaaagaaugg     1200

uuccaugaua uaccauuacc auggcacgcu ggagcagaua cuggaacacc gcauuggaau     1260

aauaaagaag cacuuguuga auucaaagac gcucaugcua aacgacaaac aguaguugua     1320

cucggaagcc aagaaggcgc aguccacaca gcucuagcag gagcguuaga agcugaaaug     1380

gauggcgcaa agggaagauu aagcucagga caccuaaaau gcagacuuaa aauggacaaa     1440

cuaagauuaa aaggaguuag uuauagccua uguacagcag cauucacauu uacuaaaaua     1500

ccagcugaaa cauuacaugg aacuguuaca guagaaguac aauaugcagg aacagauggu     1560

ccauguaaag uaccugcaca aauggcagua gauaugcaaa cauuaacacc ugucggaaga     1620

uuaauuacag caaauccugu aauaacggaa aguaccgaaa auucaaaaau gaugcuagaa     1680

uuagacccac cuuuuggcga uagcuacaua guaauuggag uaggagaaaa aaaaauaaca     1740

caucauuggc accgaagugg aagcacaaua ggaaaagcau uugaagcaac aguuagagga     1800

gcaaaaagaa uggcaguauu aggggauacc gccugggauu ucgguucugu aggaggugca     1860

cuaaauaguc uuggaaaagg cauacaccaa auuuuuggag cagcuuucaa aucacuguuu     1920

gguggaauga guugguuuuc acaaauucua auaggaacau uauuaaugug guuaggauua     1980

aauacaaaaa acggaucaau aagcuuaaug ugucuagccu uaggaggcgu auuaauauuc     2040

cuaucgacag ccguaagugc a                                               2061


<210>  2473
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS opt

<400>  2473
agucaaaaag uaauauaucu agucaugaua uuauuaauag caccagcaua uucaauaaga       60

uguauaggag ucucaaacag ggauuucgua gaaggaauga guggagguac guggguagau      120

auaguuuuag aacauggagg auguguaacc guuauggcac aagacaaacc aacaguagac      180

auagaguuag ucacaacaac aguaagcaau auggcagaag uaagaucaua cuguuaugaa      240

gcaaguauaa gugauauggc aagugacuca agauguccaa cucagggaga agcauaccua      300

gacaaacaau cagacacaca auacguaugu aaacgaacac uaguggauag aggaugggga      360

aaugguugug gacuauuugg aaaaggaagc cugguuacau gcgcuaaauu ugccugcuca      420

aaaaaaauga caggaaaauc aauacaacca gaaaaucuag aauaucggau uauguuaagc      480

guacauggaa gccaacacuc aggaaugaua guaaaugaua caggacauga aacagaugaa      540

aaucgagcaa aaguggaaau aaccccaaac agcccaagag cagaagcuac acuaggagga      600

uuuggauccu uaggacuaga uugugaaccc agaacaggac uagacuuuuc ugauuuauac      660

uaucuuacaa ugaauaauaa acauugguua guacacaaag aaugguucca ugauauacca      720

uuaccauggc augcaggagc agauacgggc acaccacacu ggaauaauaa agaagcauua      780

guagaauuua aagaugcaca cgcaaaaaga caaacaguag uaguauuagg aucacaagaa      840

ggcgcaguuc auacagcccu agcaggagca cuagaagcag aaauggaugg agcaaaagga      900

aggcuuagca gcggucauuu aaaaugcaga uuaaaaaugg auaaauuaag acuaaaagga      960

guaaguuaua gucuauguac agccgcauuu acauuuacaa aaauaccagc cgaaacuuua     1020

cauggaacag uaacaguaga gguacaauau gcaggaacgg acggaccaug uaaaguacca     1080

gcacaaaugg cuguagacau gcaaacacua accccaguag gcagauuaau aacagcuaau     1140

ccaguuauaa cagaaaguac agaaaauucu aaaaugaugc uagaauuaga cccaccuuuu     1200

ggagacucau auauaguaau agguguagga gaaaaaaaaa uaacacauca uuggcauaga     1260

ucaggaagua caauaggaaa agcauucgaa gcaaccguaa gaggagccaa aagaauggcg     1320

guacuaggug auacagcaug ggauuuuggc uca                                  1353


<210>  2474
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS opt

<400>  2474
agucaaaaag uuauauauuu aguuaugauu uuacuuauag cuccggcaua caguauaaga       60

uguauaggag uaucaaauag agauuucgua gagggaauga guggaggaac auggguagau      120

guaguauuag aacauggagg uuguguaaca guaauggcac aagacaaacc aacaguagau      180

auagaauuag uuacaaccac aguaucaaau auggcagaag uaagaagcua uugcuaugaa      240

gcaaguauaa gugacauggc aagugauagc agauguccaa cacaaggaga agcauauuua      300

gauaaacaaa gugauacaca auacguuugu aaaagaacau uaguagauag agguugggga      360

aacggaugug guuuauuugg aaaagggucu uuaguaacuu gugcgaaauu ugcuugcagu      420

aaaaaaauga caggaaaauc aauacaacca gaaaauuuag aauauaggau aauguuauca      480

guacauggau cucaacauag cgguaugaua guaaaugaca caggacauga aacagaugaa      540

aauagagcaa aaguagaaau aacaccgaau ucgcccagag cagaagcaac acugggagga      600

uuuggaagcc uaggacuaga uugugaacca agaacgggac uagauuuuuc agaucuguau      660

uaucuuacaa ugaauaauaa acauuggcua gugcauaaag agugguuuca ugauauacca      720

cuaccauggc augcaggagc agacacaggu acaccacauu ggaauaauaa agaagcacua      780

guagaauuca aagacgcaca ugcaaaaaga caaacaguug uuguacuagg aagccaagaa      840

ggagcaguac auacagcauu agcaggggca uuagaagcag aaauggacgg agcaaaaggu      900

agacuaucga gcggacaccu aaaauguaga uuaaaaaugg auaaacuacg auuaaaagga      960

guaaguuaca guuuaugcac agcugcauuu acauuuacaa aaauaccggc agaaacauua     1020

cauggaacgg uuacaguaga aguacaauac gcuggaacag auggacccug uaaagugccu     1080

gcgcaaaugg caguggacau gcaaacauug acaccaguag gaagacuaau aacagcaaau     1140

ccaguaauaa cagaaaguac ggaaaauagu aaaaugaugu uagaacuaga uccuccauuu     1200

ggagacucau auauaguaau aggaguagga gaaaaaaaaa ucacacacca cuggcacaga     1260

ucaggaucga caauaggaaa agccuuugaa gcaacaguaa gaggagcaaa aagaauggcu     1320

guauuagggg acacagcaug ggacuuugga agu                                  1353


<210>  2475
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS opt

<400>  2475
ucacaaaaag uaauuuaucu aguuaugaua uuauuaauag cuccagcaua caguauaaga       60

uguauaggag uaucaaacag agacuuugua gaaggaaugu caggaggaac auggguugac      120

guaguauuag aacacggugg augcguaaca guaauggcac aggacaaacc aacaguugau      180

auagaauuag uuacaacaac aguuucaaau auggcugaag uaagaucaua uuguuaugaa      240

gcaaguauaa gugacauggc cagcgacagu agaugcccua cacaaggaga agcauaccua      300

gauaaacaaa gcgauacaca auacgucugc aaaagaaccc uaguagacag agguuggggc      360

aacgguugug gacuauuugg uaaagguagc uugguaacau gugcaaaauu uacauguuca      420

aagaaaauga caggaaaauc aauacaaccc gaaaaucuug aauauagaau aaugcugagu      480

guacauggaa gucaacauuc aggaaugauc ggauacgaaa cagacgaaga uagagcaaaa      540

guagaaguaa caccaaauuc accgagagca gaagcaacac uaggaggauu uggcucacua      600

ggauuagacu gugaaccaag aacaggacua gauuucagug aucuauacua ccuaacaaug      660

aacaacaaac acugguuagu acauaaagaa ugguuucaug acauaccauu accuuggcac      720

gcaggagcag auacaggaac accacauugg aauaauaaag aagcauuagu ggaauuuaaa      780

gaugcucaug caaaaagaca aacaguagua guacuaggaa gucaagaagg cgccguacac      840

acagcucuag caggagcauu agaagcagaa auggauggag caaaaggaag auuauuuagu      900

ggacacuuaa aauguagauu aaaaauggau aaacuaagau uaaaaggugu aucauauagu      960

uuauguacag cagcuuucac auuuacaaaa guaccagcag aaacauuaca uggaacaguc     1020

acaguagaag uacaauacgc aggaacagau ggaccaugua aaauaccagu acaaauggca     1080

guagacaugc aaacacuaac uccaguagga agguuaauaa cagcaaaucc aguaauaaca     1140

gaaaguacag aaaauaguaa aaugauguua gaauuagauc ccccauuugg agacaguuau     1200

auagugauag gcguaggaga caagaaaaua acacaucauu ggcauagauc agguucaacu     1260

auaggaaaag cauuugaagc uacaguaaga ggugcgaaaa gaauggcagu acuaggagau     1320

acagcauggg acuuuggaag u                                               1341


<210>  2476
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) CDS opt

<400>  2476
agucaaaaag uuauauauuu gguaaugaua uuacuaauug cuccagcaua uucaauacga       60

ugcauaggag uaucaaauag agauuucguc gaaggaauga guggaggaac auggguagau      120

guaguauuag aacauggagg augcguaaca guaauggcac aagauaaacc aacaguagau      180

auagaacuag uaacaacaac cguaucaaac auggcagaag uaagaucaua uuguuaugaa      240

gcaucaauaa gcgacauggc gucugauagu agaugcccaa cacaagguga ggcauauuua      300

gauaaacaaa gcgauacaca auauguuugc aaaagaacau uagucgauag aggaugggga      360

aauggaugug gauuguucgg aaaaggauca cuagucacau gugcaaaguu ugcauguagu      420

aaaaaaauga caggaaaaag uauccaacca gaaaacuuag aauauagaau aaugcucagu      480

guacauggua gucaacauag uggcaugaua gugaaugaua caggucauga aacagaugaa      540

aaucgagcga aaguagaaau uacacccaau ucuccuagag cagaagcaac uuuaggagga      600

uuuggaaguu uaggauuaga uugcgaacca agaacaggau uagacuucag cgaucuauac      660

uauuuaacaa ugaacaauaa acauuggcuc guacauaaag aaugguuuca ugacauaccu      720

cuaccuuggc acgcaggagc agauacagga acuccacauu ggaauaauaa agaagccuua      780

guagaauuua aagaugcaca ugcaaaaaga caaacaguag ucguacuagg aagccaagaa      840

ggagcaguac auacugcauu ggcaggagca uuagaagcag aaauggacgg ugcaaaagga      900

agguuaucaa guggacaucu aaaaugcaga cuuaaaaugg auaaauuaag auuaaaagga      960

guaaguuauu cauuaugcac agcagcauuu acauuuacaa aaaucccagc agaaacacua     1020

cauggaacag uaacaguaga aguacaauac gcaggaacug auggaccaug uaaaguacca     1080

gcacaaaugg ccguugauau gcaaacauua acaccaguag gaagauuaau aacagcaaau     1140

ccaguaauaa cagaaaguac cgaaaacagu aaaaugaugu uagaacuaga uccuccauuu     1200

ggagacaguu acauaguuau aggaguagga gaaaaaaaaa uaacacacca uuggcacaga     1260

aguggaagca caauagguaa agcauuugaa gcaacaguaa ggggagcaaa aagaauggca     1320

guacuaggag auacagcaug ggauuucgga uca                                  1353


<210>  2477
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) CDS opt

<400>  2477
acaucaguag gaauuguugg auuauuacua acaacagcaa uggcagcaga aguaacaaga       60

cgaggaagcg cauauuauau guaucuggau agaaaugaug cuggugaagc aauaaguuuu      120

ccaacaaccu uagguaugaa uaaauguuac auucaaauca uggauuuagg acacaugugu      180

gaugcaacaa ugucauauga auguccuaug cuagaugaag gcguugaacc agacgaugug      240

gauuguuggu gcaacacaac aaguacaugg gugguauaug guacguguca ucacaagaaa      300

ggagaagcaa gaagaucaag gagagcaguu acacuuccaa gccauaguac aagaaaauua      360

caaacaagaa gucaaacaug guuagaauca agagaguaca caaaacaccu aauuagagua      420

gaaaauugga uauucagaaa uccaggguuc gcacucgcag cagcagcuau agcaugguua      480

cuaggaagua guacaaguca aaaaguaaua uaucuaguaa ugauacuauu gaucgcgcca      540

gcauauucua uaagauguau aggaguaagc aauagagauu uuguagaagg uaugucugga      600

gguacauggg uagauauagu auuagaacac ggcggaugcg ucacaguaau ggcccaagau      660

aaaccaacag uagauauaga acucguaaca acaacaguua guaauauggc agaaguaaga      720

agcuacuguu acgaagccag uauaucagac auggcaagug auucaagaug uccaacacag      780

ggagaagcau auuuagauaa acaaucugac acacaauaug uuugcaaaag aaccuuagua      840

gacagaggau ggggaaacgg augcggacua agcggaaaag guagcuuagu aacaugugca      900

aaauucgcau gcucaaagaa aaugacugga aaaagcauac aaccagaaaa cuuagaauau      960

agaauuaugu uaaguguaca uggaucacaa cacaguggaa ugauaguaaa cgacacugga     1020

caugaaaccg augaaaaucg agcaaaagua gaaauaacac cuaauagccc gagagcggaa     1080

gccacacuag gaggauucgg aaguuuagga cuagacugug aaccaagaac aggauuagac     1140

uuuagugauc uauauuauuu aaccaugaac aauaaacauu gguuaguuca uaaagaaugg     1200

uuccaugaca uaccauuacc auggcacgca ggagcagaua ccgguacgcc gcauuggaau     1260

aacaaagaag cucuaguaga auuuaaagau gcacaugcaa aacgacaaac uguaguggua     1320

cuaggaucac aagaaggagc aguucauaca gcuuuagcgg gggcgcucga agcagagaug     1380

gacggugcaa aagguagauu aaguucagga caccuaaaau guagacuaaa aauggauaaa     1440

cuaagauuaa aaggaguauc guauucuuua uguacagcug cguuuaccuu uacaaaaauu     1500

ccagcagaaa cauuacaugg aaccgucaca guugaagugc aauacgcagg aacugauggu     1560

ccaugcaaag ucccugcaca gauggcagua gauaugcaaa cgcuaacacc gguaggacga     1620

uugaucacug cgaauccugu gauaacugaa agcaccgaaa auucaaaaau gaugcuggaa     1680

uuagauccuc cauuuggaga caguuacaua guaauaggag uaggagaaaa gaaaauaaca     1740

caucauuggc acagaucagg aaguacaaua ggcaaagcau uugaagcuac agugagggga     1800

gcaaaacgaa uggcaguauu aggugauaca gcaugggacu uuggcagugu aggaggggca     1860

cuaaauucuu ugggaaaagg uauacaucaa aucuuuggag cagcauuuaa aaguuuauuu     1920

ggaggaauga gcugguuuuc acaaauuuug auaggcacuu uauuaaugug guuaggacua     1980

aauacuaaaa auggaucaau aucauuaaug uguuuagcuu uaggaggcgu auuaauauuu     2040

uuauccacag cagugagcgc u                                               2061


<210>  2478
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) CDS opt

<400>  2478
acauccguag gaauuguagg auuauuacua acaaccgcaa uggcagcaga aguaacgcga       60

cgaggaagug cauauuacau guacuuagau agaaaugacg cuggugaagc aauaaguuuu      120

ccuacaacuc uugggaugaa caaauguuac auccaaauaa uggaucuugg ccauacaugu      180

gaugcaacaa ugucauauga auguccaaug uuagaugaag gagucgaacc cgacgauguc      240

gauuguuggu gcaauaccac aucaacaugg guuguauaug gaacaugcca ucauaagaaa      300

ggagaagcgc ggagaaguag acgagcagua acauuaccau cacauucgac acgaaaauua      360

caaacaagaa gucaaacaug guuagaaagc agagaauaca caaaacauuu aauaagagua      420

gaaaacugga uauuuagaaa uccaggguuu gcauuagcag cagcagcaau agcauggcuu      480

cuaggaucca guaccucaca aaaaguaaua uacuuaguaa ugauauuauu gauugcacca      540

gcuuauucua uaagauguau uggaguuagc aauagagauu uuguagaagg gaugagugga      600

ggaacauggg uagauguagu uuuagaacac ggagguugug uaacaguaau ggcacaagau      660

aaaccaacag uagauauaga gcuaguaaca acuacuguga gcaauauggc agaaguucga      720

ucauacuguu acgaagcauc aauaagugac auggcaucgg auagcagaug uccaacacaa      780

ggugaagcau aucuagauaa acaaucagau acucaauaug uauguaaacg aacuuuagua      840

gauagaggau ggggaaaugg augcgguuua agcggaaaag gcucauuagu uacaugugcu      900

aaauucgcuu guaguaagaa aaugacagga aaaucaauac aacccgaaaa ucuagaauau      960

cgaauaaugu uauccguaca uggaagccaa cauucaggua ugauaguaaa ugacacagga     1020

caugaaacag acgaaaauag agcuaaagua gaaauaacuc cuaauucacc aagagcagaa     1080

gcuacacuag gagguuucgg uagccuaggg uuagauugug aaccaagaac aggauuagac     1140

uuuucagacu uauauuaccu aacaaugaau aauaaacauu gguuaguuca caaagaaugg     1200

uuucacgaua uaccauugcc uuggcaugca ggagcagaua cuggaacacc acacuggaau     1260

aauaaagaag cauuaguaga auuuaaagau gcacacgcaa aaagacagac aguugucgua     1320

uuagggucac aagaaggagc uguacauaca gcauuagccg gagccuuaga agcagaaaug     1380

gacggagcaa aaggaagacu aaguagcgga caucuuaaau guagacuaaa aauggacaaa     1440

cuacgacuga aaggaguauc auauucauua uguacagcag cuuuuacauu cacaaaaaua     1500

ccagcugaaa cccuacaugg aacaguaaca gucgaaguuc aauacgcagg aacggacgga     1560

ccauguaaag uaccagcuca aauggcagua gacaugcaga cuuuaacacc aguagggagg     1620

cuaauuacag caaacccugu uauaacagaa ucaacggaaa acucaaaaau gauguuggaa     1680

uuagauccac cuuuuggaga cucauacaua guaauaggag uaggagaaaa gaaaauaaca     1740

caucacuggc aucgaagugg aucaacuauu gguaaagcau uugaagcaac cguaagagga     1800

gcuaaaagaa uggcuguuuu aggugauaca gcgugggauu uuggcucagu gggaggugca     1860

uuaaacucuu uaggcaaagg gauacaccaa auauuuggug ccgcauuuaa aucauuauuc     1920

ggaggcaugu caugguucag ccaaauauua auaggaacau uauuaaugug guuaggucua     1980

aacgcaaaga acggaaguau uucucuaaug uguuuagcau uaggaggagu ucuaauauuu     2040

cuaucaaccg caguuucagc a                                               2061


<210>  2479
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) CDS opt

<400>  2479
acaucaauug gaauaaucgg cuuauugcua acuacagcaa uggcagcuga aauuacaaga       60

agaggaucag cuuauuauau guaucuagac agaucagaug cugguaaagc aauuucauuc      120

gcaacaacac uaggcguaaa uaaaugucac guacaaauaa uggauuuagg ccauaugugc      180

gaugcaacaa ugucguauga auguccaaug uuagacgaag gaguggaacc cgaugaugua      240

gacuguuggu gcaacacaac uucaacaugg guaguauacg gaacaugcca ucacaaaaaa      300

ggagaagcaa gaagaucaag acgagcaguu acucuaccaa gccauaguac aaggaaacua      360

caaaccagau cacaaacaug gcuagaauca cgugaauaca caaagcauuu aauaaaagua      420

gaaaacugga uauuuagaaa uccaggguuc gcauuagugg caguagcaau agcauggcua      480

uuaggaucau caacaucaca aaaaguaaua uauuuaguaa ugauauuauu aauagcgcca      540

gcauauagua uacgauguau aggaguauca aacagagauu uuguggaagg aaugucagga      600

gguacauggg uggacguagu ucuagaacac ggaggcugug uaacaguaau ggcacaagac      660

aaaccuaccg uagauauaga acuagugacc acaacaguau caaacauggc ggaaguaagg      720

ucuuauugcu augaagccag uauaucagau auggcaucug auagcagaug cccaacacaa      780

ggagaagcau aucuugauaa acaaucugau acacaauaug uauguaaaag aaccuuagua      840

gaucgaggau ggggaaaugg uuguggacua ucagguaaag guucauuagu cacaugugcu      900

aaauucacuu guaguaaaaa aaugacagga aaaucuauac aaccagaaaa ucuugaauau      960

aggauaaugu uaagugucca cggaagucaa cauucaggga ugauagguua ugaaacagau     1020

gaagacaggg caaaaguaga aguaacacca aacagcccaa gagcggaagc aacauuagga     1080

ggauucggaa gccucggacu agacugugaa ccacggacgg gauuagacuu cagcgaucuu     1140

uauuauuuaa caaugaauaa uaaacacugg cuaguucaua aagaaugguu ccaugauauc     1200

ccucuaccau ggcaugcagg agcagauaca ggaacacccc acuggaauaa uaaagaagcc     1260

uuaguagaau uuaaagaugc ccacgccaaa agacaaacgg uaguuguauu aggaucacaa     1320

gaaggagcag uacauacggc guuagcuggc gcccuagaag cagaaaugga uggugcgaaa     1380

ggaagacuau uuucaggcca ucucaaaugc agauugaaaa uggauaaacu uagacuaaaa     1440

ggaguaucau auucauuaug uacagcagca uuuacauuua ccaaaguacc agcagaaaca     1500

uuacauggaa caguuacagu agaaguucaa uaugcaggua cagauggacc uuguaagaua     1560

ccaguacaaa uggcgguaga uaugcaaaca cucacuccag uugguagauu aauuacugca     1620

aacccaguaa uaacagaauc gacggaaaau ucuaaaauga uguuagaauu agacccacca     1680

uuuggagaua guuauauagu gauaggugug ggagauaaaa aaauaacaca ucauuggcau     1740

cgaucagggu caacuauagg uaaagcauuc gaagcaacag ugagaggggc aaaaaggaug     1800

gcaguacuag gagauacugc augggauuuu ggcagugucg gugggguuuu uaauagccua     1860

gguaaaggaa uacaccaaau auuuggcgca gcguucaaau cauuauuugg cggaauguca     1920

ugguuuucuc aaauacuuau aggaaccuua cuaguauggu uggguuuaaa caccaaaaau     1980

ggaaguauuu cacuaacaug uuuagcccug ggaggaguaa ugauauuucu aagcacagca     2040

guaagugca                                                             2049


<210>  2480
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) CDS opt

<400>  2480
acaucaguag gaauaguagg acuacuauua acaacagcaa uggcagcuga gguaacaaga       60

agaggaagcg cuuauuauau guauuuagau agaaaugaug caggagaagc uaucagcuuc      120

ccaacaacac uaggaaugaa caaauguuau auacaaauaa uggauuuagg ccacaugugc      180

gaugcaacaa ugagcuauga auguccaaug uuagaugaag gaguagaacc ugaugauguu      240

gauuguuggu gcaacaccac aaguacaugg guaguuuaug gaacaugcca ucacaagaaa      300

ggugaagcac gaagaucaag acgagcagua acacugccau cucauucaac acgaaaauua      360

cagacaagaa gucagacaug gcuugaaagu agagaauaua cgaaacaccu aauacgcgua      420

gaaaauugga uauuuagaaa cccuggauuu gccuuggcgg cagcagcaau agcuugguua      480

uuagguucua guacaucaca aaaaguaaua uaucuaguaa ugauacuacu aauagcacca      540

gcauacucaa uaagaugcau aggaguaagc aacagagauu ucguagaagg aaugagugga      600

ggaaccuggg uagauguagu ccuagagcac ggaggaugug uaacaguaau ggcacaagac      660

aaaccaacug uagauauaga acuuguaaca acaacgguau caaacauggc agaaguacga      720

aguuacugcu acgaagcauc aauaucugac auggcuucag auucaagaug cccaacacaa      780

ggagaagccu aucuagauaa acaaucagac acacaauacg uuugcaaacg aacauuagua      840

gaccgagguu ggggaaacgg augugggcua ucagggaaag guucacuugu aacaugugca      900

aaauuugcau gcucaaagaa aaugaccggu aaaaguauuc aaccagaaaa ucuagaauac      960

agaauuaugc uaucaguaca cggaucacaa cauaguggua ugauaguaaa ugacacugga     1020

caugagacug augaaaauag agcaaaagua gaaauaacac caaauagucc aagagcagaa     1080

gcaacauuag gaggauuugg uaguuuagga cucgacugug aaccacggac ugggcuggau     1140

uuuagugacu uauauuacuu aaccaugaau aauaaacacu gguuaguaca caaagaaugg     1200

uuccaugaua uaccuuugcc auggcaugcu ggugcagaua caggaacucc ccauuggaau     1260

aauaaagaag cacuuguaga auuuaaagau gcacacgcaa aacgacaaac aguaguugua     1320

cuagguuccc aagaaggagc aguacauaca gcacuugcag gagcucuaga agcugaaaug     1380

gauggagcua aaggacgauu aaguagugga caccuaaaau guagacuuaa aauggacaaa     1440

uuaagguuaa aaggaguaag uuauucauua uguacagcag cauucaccuu cacuaaaaua     1500

ccugcggaaa cacuccacgg uacaguaacu guagaaguac aauaugcagg aacagaugga     1560

ccauguaaag uaccagcaca aauggcugug gauaugcaaa cacuaacucc cguaggaaga     1620

cuaauaacug caaacccagu aaucaccgag aguacagaaa acucuaaaau gauguuagaa     1680

uuagauccac cauuuggaga uucauacauu guaauuggag uaggugaaaa gaaaauaacu     1740

caccauuggc auagaucugg aaguacaaua ggaaaagccu ucgaagcaac aguaagaggu     1800

gcuaaacgga uggcaguauu aggagauacg gcaugggauu uugguucugu agguggagca     1860

cuaaacuccc uagggaaagg gauucaccaa auuuuuggag cagcauuuaa aucauuauuu     1920

ggaggcauga gcugguuuuc acagauacua auagguacau uacuuaugug guuggguuua     1980

aacacuaaaa acgguucaau uucauuaaug ugcuuagcac uagguggugu guuaauauuu     2040

cuaaguacag cgguuagugc u                                               2061


<210>  2481
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) CDS opt

<400>  2481
accagcguag gaauuguagg gcucuuauua acaacagcua uggcagcaga agugacaaga       60

agggguagug ccuauuacau guacuuagau agaaaugacg caggugaagc aauuucauuu      120

ccaacaacau uaggaaugaa uaaauguuac auacaaauaa uggaccuagg acauaugugu      180

gacgcaacaa ugaguuauga augucccaug uuagacgagg ggguugaacc agaugaugua      240

gauugcuggu guaauacaac gaguacgugg guaguauaug gaacguguca ucauaaaaaa      300

ggagaagcuc guagaaguag gagagcagua acuuuaccaa gucacucaac aagaaaauua      360

caaacaagaa gccaaacaug guuagaauca agagaauaua caaaacaucu cauaagagua      420

gaaaauugga uauuuagaaa uccaggauuu gcauuagcag cugcugcaau ugccugguua      480

cucggaaguu ccaccucaca aaaaguaaua uaccucguaa ugaucuuguu aauagcacca      540

gcuuauucga uaagauguau cggaguuagu aauagagauu uuguugaagg caugagcgga      600

gggacauggg uagauauagu acuugaacau ggcggaugug uuacaguaau ggcacaagau      660

aaaccaacgg uagauauuga auuaguaaca acaacaguca guaacauggc ugaaguaaga      720

ucauauugcu augaagcuag uauaucugau auggcuagug auaguagaug uccaacucaa      780

ggagaagccu auuuagauaa acaaagugac acucaauaug uuuguaaaag aacauuagua      840

gacagaggau ggggaaaugg uuguggauua uucggaaaag gaagcuuggu aacaugugca      900

aaauucgcau guaguaaaaa aaugacagga aaaucuauuc aaccagaaaa cuuagaauau      960

aggauaaugu uaucagugca cggcucacaa cauaguggaa ugauaguaca agacacaggg     1020

caugaaacag augaaaauag agcaaaagua gaaauaacuc caaacagucc uagagcugaa     1080

gcaacucuag guggauuugg aaguuuaggc uuagauugug aaccaaggac aggauuagau     1140

uuuagugauu uauauuaccu aacaaugaau aauaaacauu ggcuuguaca uaaagaaugg     1200

uuccaugaua uuccacuacc auggcaugca ggagcagaca cugguacucc acauuggaau     1260

aacaaagaag cauuaguuga auuuaaagau gcccaugcua aaagacaaac aguaguagua     1320

uuaggaaguc aagaaggagc uguucacacu gcacuugcag gagcacuaga agcagaaaug     1380

gauggagcaa aaggacgguu aagcagugga cauuuaaaau gcagacuaaa aauggauaaa     1440

cuucgauuaa aagggguauc auauaguuua uguacugcag cuuuuacguu uacaaaaaua     1500

ccagcggaaa cacuacaugg aacaguuaca guugaaguac aguaugcagg cacugaugga     1560

ccuuguaagg uaccagcgca aauggcagua gacaugcaaa cacuaacucc agugggacga     1620

cuaauaacag caaauccagu aauaacagaa agcacagaaa auaguaaaau gaugcuagaa     1680

cuagacccac cauuuggaga cucauacaua gucauaggcg uaggagaaaa aaaaauaacc     1740

caucauuggc acagaagugg aucaacuaua ggaaaagcau uugaagcaac aguucgugga     1800

gcaaagagaa uggcaguauu gggagacacc gcaugggauu ucgguagugu uggaggugca     1860

cuuaauucau ugggaaaagg aauacaucaa auuuuuggag cagcuuucaa aaguuuauuc     1920

ggcgggaugu caugguucag ucaaauauua auagguacuu uacuaaugug guuaggauua     1980

aauacaaaaa augguagcau uucacuuaug ugucuagcau uaggaggugu uuugauauuu     2040

cuaucaacag cugugucagc u                                               2061


<210>  2482
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) CDS opt

<400>  2482
acaucagucg gaauagucgg acuucuauua acuacugcaa uggcagcaga agucacaaga       60

agaggaucag cuuauuacau guacuuagau aggaaugaug cuggagaagc cauuagcuuu      120

cccaccacuu uaggcaugaa uaaauguuac auacaaauaa uggauuuagg acauacaugu      180

gaugcaacaa ugucauacga augucccaug uuagaugaag guguagaacc agaugauguu      240

gauuguuggu gcaauacaac aucaacaugg guaguauacg gaacaugcca ucauaagaaa      300

ggagaagcaa ggcgaucaag acgagcaguu acauugccua gucauaguac cagaaaauua      360

caaacaagau cucaaacaug guuagaaagu agagaauaca caaaacaucu aauaagaguu      420

gaaaacugga uauucagaaa uccaggauuu gcacuagcag cagcagccau agcauggcua      480

uuaggaucaa gcacaaguca aaaaguuaua uacuuaguga ugauauuauu aauagcacca      540

gcuuacucua uaagauguau cggagucucc aacagagauu uuguagaagg aaugucagga      600

ggaacauggg uugauguagu auuagaacau gguggaugug uaacaguaau ggcacaagau      660

aaaccaaccg uagauauaga auuagucaca acaacuguau caaauauggc cgaaguaaga      720

aguuacuguu augaagcauc aauuucugac auggcuucag auagccgcug ccccacacaa      780

ggcgaggcau auuuagacaa acaaagcgau acacaauaug uauguaaaag aacucuaguu      840

gauagaggau gggguaaugg auguggacua uucgguaaag guucauuagu aacaugugca      900

aaauuugcuu guagcaagaa aaugacaggg aaaucaaucc aaccagaaaa ucuagaauau      960

agaauaaugu uaucagugca cggaagucag cacucaggua ugauaguuca agauacagga     1020

caugaaacag acgaaaauag agcaaaagug gaaaucacgc caaauucacc aagagcugaa     1080

gcuacccuag guggcuuugg aucauuaggc uuagauugcg aaccaaggac aggauuagau     1140

uuuucagauc ucuacuauuu aacaaugaau aauaaacacu ggcuagucca caaagaaugg     1200

uuccaugaua ucccauugcc uuggcaugcu ggagcagaua cagguacacc acauuggaau     1260

aacaaagaag cacucguaga guucaaagau gcacaugcaa aaagacaaac aguaguagua     1320

cuaggauccc aagaaggugc aguucacacu gcacuggcag gagccuuaga agcagaaaug     1380

gauggagcaa aaggaagauu aucaagugga caccuaaaau guagauuaaa aauggauaaa     1440

cuaagauuaa aaggaguauc cuauucccuc ugcaccgcag ccuucacuuu uacuaaaaua     1500

ccagcagaaa cauugcaugg aacaguuacc guagaaguac aauacgcagg aacugaugga     1560

cccuguaaag uacccgcaca aauggcagua gauaugcaaa cacucacacc aguaggaaga     1620

uuaauaacag caaauccagu aauaaccgaa ucaacagaaa acagcaaaau gaugcuugaa     1680

uuagauccac cuuuuggaga uucauauaua guaauuggug uaggcgaaaa gaaaaucaca     1740

caucauuggc auagaagugg aucaaccaua ggaaaagcau uugaagcaac aguaagaggg     1800

gcaaaacgaa uggcgguccu aggagacacg gcaugggauu uuggaucagu aggaggagca     1860

uuaaauucac uaggaaaagg cauucaucaa auauucggag cugcauucaa aaguuuauuc     1920

ggaggaaugu caugguuuag ucaaauacuu auaggcacau uauuaaugug gcuaggauua     1980

aacgccaaaa augggucaau aucacucaug uguuuagcuu uagguggagu auuaauauuu     2040

uuauccacag caguaucugc a                                               2061


<210>  2483
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) CDS opt

<400>  2483
acaagcgugg gaauaguugg acuacuauua acaacagcaa uggcagcaga agugacucgu       60

cggggaagug cauauuauau guauuuagau agaaaugacg caggagaagc aauuaguuuu      120

ccaacaacau uaggaaugaa uaaauguuac auacaaauua uggaucuagg acacaugugu      180

gaugcaacaa ugucauauga auguccgaug cuagacgaag gaguagagcc agaugauguu      240

gacugcuggu gcaauacaac aagcacaugg guagucuacg gaacuugcca ucauaagaaa      300

ggagaggcac gaagaagccg gagagcugua acuuuaccua gccauucuac ucgaaaauua      360

caaacaagau cacaaacuug gcuagaaagu cgagaauaua caaaacauuu aauaagagua      420

gaaaacugga uauuccguaa uccuggguuu gcucuugcag cagcagcaau agcguggcua      480

cuaggaagua gcacaaguca aaaaguaaua uacuuaguga ugauauuauu aauagcucca      540

gcauauucaa uuagauguau cggugugagu aauagagauu ucguagaagg aaugagugga      600

gggacuuggg uagauguagu uuuagaacac ggaggaugug ucacagugau ggcucaagac      660

aaaccaacug uagacauaga acuuguaacu accacaguau caaacauggc agaaguuaga      720

aguuauuguu augaagcauc uauaucagau auggcaucag auucacgaug uccaacacaa      780

ggcgaagcgu aucuagauaa acaaucggau acucaauaug uauguaaaag aacuuuagua      840

gauagaggau ggggaaaugg auguggccua uucggaaaag gaucauuagu gacaugcgcu      900

aaauuugccu guagcaagaa aaugaccggu aaaaguauuc aaccagaaaa uuuagaauau      960

agaauaaugu uaucaguuca cggaucacaa cauaguggaa ugauaguuca agauacaggc     1020

cacgaaacug acgaaaauag agcaaaagua gaaauaacac caaauucucc aagagcagaa     1080

gcaacacuag gcggauuugg aucauuagga uuggauugug aaccucgaac aggauuagac     1140

uuuagugauu uguauuauuu aacaaugaac aacaaacauu gguuaguaca caaagaaugg     1200

uuucacgaua uaccacuacc auggcaugca ggagcagaua cgggaacacc ccauuggaau     1260

aauaaagaag cgcuuguaga auuuaaagau gcucaugcaa aaaggcaaac aguaguagua     1320

uuaggaaguc aagaaggagc aguacauaca gcucuagcug gagcccugga agcugaaaug     1380

gauggggcua aaggaagacu uagcucagga caucuaaagu gccgauuaaa gauggacaaa     1440

uuaagauuaa aaggcguaag uuauucuuua uguacagcag cauuuacauu uacaaaaaua     1500

ccagcagaaa cgcugcaugg uacuguaaca guagaaguac aauaugcagg aacugacgga     1560

ccauguaaag uaccagcaca aauggcgguu gauaugcaaa cauuaacacc uguaggaaga     1620

cucauuacag caaacccagu uauaacagaa ucuacagaaa auuccaagau gaugcuggag     1680

uuagaucccc cauuugguga uaguuauaua guuaucggug uaggugagaa gaaaauaaca     1740

caucacuggc accgaagugg aucaacaaua gguaaagcau ucgaagcaac aguaagagga     1800

gcaaaaagga uggcaguauu aggugacaca gccugggauu uuggaucugu aggaggagca     1860

cuuaauucau uaggaaaagg cauucaccaa auauuuggag cagcauuuaa aucauuauuc     1920

gguggaaugu caugguuuuc acaaauuuug auagguacac uacuaaugug gcuaggacua     1980

aacaccaaaa augggucaau aagucuaaug ugcuuagcgu uaggaggagu acuaauauuc     2040

cuaaguacag caguaucagc a                                               2061


<210>  2484
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2484
acauccguag guauaguagg uuuacuauua acuacugcga uggccgcaga agucacaaga       60

agagguucag cuuacuauau guaucuagau cguaaugacg caggagaagc aauaucauuc      120

ccuacaacau uagguaugaa uaaauguuau auccaaauaa uggauuuagg gcauaugugu      180

gaugcaacaa ugaguuauga augcccaaug cuagaugaag gaguagaacc agacgacguu      240

gacugcuggu guaauacaac aagcacaugg guaguauacg gaacauguca ccauaaaaaa      300

ggagaagcuc gaaggucacg acgagcgguu acauugccau cucauaguac aagaaaacua      360

cagacaagau cacagacaug gcuagaaagu agagaauaca caaaacaucu aauucgagua      420

gaaaacugga uauuuagaaa uccaggcuuu gcacucgcag cagcugcaau agcauggcuc      480

uugggaaguu caacaucaca gaaaguaaua uaccuaguaa ugauauuacu uauagcacca      540

gccuauagua uuagguguau aggaguaucu aauagagauu uuguagaagg aaugagcgga      600

gggacauggg uagauauagu acuagaacau ggaggaugug uaacuguaau ggcacaagau      660

aaaccaacag uugacauaga acuaguaaca acaacaguau caaauauggc agaaguaaga      720

aguuauuguu augaagcgag uauaagugau auggcaucag auucaagaug cccuacacaa      780

ggagaagcau accuagacaa acaaucggau acacaauaug uauguaaaag gacauuaguu      840

gauagagguu gggggaacgg auguggguua uucggaaaag gaaguuuagu aaccugcgcc      900

aaauuugcau guaguaagaa aaugacaggg aaaaguaucc aaccugaaaa ucuggaauac      960

agaauuaugu uaagugugca uggaagucaa cacaguggaa ugauaguaaa ugauacggga     1020

cacgaaacag augaaaauag ggcaaaaguc gaaauuacac caaacagccc cagagcagaa     1080

gcaaccuuag gaggauuugg aucauuagga uuagauugcg aaccaagaac cggauuagac     1140

uuuagcgauc uguacuauuu gacaaugaau aauaaacauu ggcuaguaca uaaagaaugg     1200

uuccaugaua uaccauuacc auggcacgca ggugcugaua cuggcacacc acauuggaac     1260

aacaaagaag cacucguuga auucaaagac gcccaugcaa aacgacaaac aguagucguu     1320

uuaggaucac aagaaggagc cguacacacu gcauuagcag gagcauuaga agcagaaaug     1380

gauggcgcca aagggagacu aucgaguggu caucuuaagu gcagacuaaa aauggauaaa     1440

uuaagguuaa aaggaguaag uuacucucua ugcacagcag cauuuacauu uacuaaaaua     1500

ccugcugaga cauuacaugg aacuguaacu guagaaguac aauacgcagg gacugaugga     1560

ccauguaaag ugccagcaca aauggcagua gauaugcaaa cauuaacacc aguaggacga     1620

uuaauaacgg caaauccagu aaucacugaa ucaacugaaa auaguaaaau gauguuagag     1680

uuagauccac cauucggcga uaguuauaua guaauaggag ugggcgaaaa aaaaaucacu     1740

caucauuggc acagaagcgg caguacauua ggaaaagcau ucagcacaac auuaaaagga     1800

gcucaaagau uagcagcacu aggugauaca gcaugggauu ucggaucaau uggaggagua     1860

uucaauucaa uagggagagc agugcaucaa guauucggug gcgcauuuag aacucuguuc     1920

ggaggaauga guuggauaac acaaggacua augggugccu uauuauuaug gaugggagua     1980

aaugcacgag accgaucaau agcacuagca uuucuagcaa cuggaggugu auuaguauuu     2040

cuggcaacua acguccacgc a                                               2061


<210>  2485
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2485
accaguguag gaauaguagg uuuacuccuu accacagcaa uggcggcaga aguaacacgg       60

agaggaucug cauauuacau guaccuugac agaaaugacg cgggagaagc aauaaguuuc      120

ccaacaacac uagggaugaa uaaaugcuac auucaaauua uggaucuagg acauacaugu      180

gaugcgacga ugagcuauga augcccaaug uuagaugaag guguagaacc agaugaugua      240

gacugcuggu guaauacuac aucgaccugg guuguauaug gaacauguca ucauaagaaa      300

ggagaagcac gaagaagcag gagagcagua acauuaccca gucauucaac aagaaaauua      360

caaaccagau cacaaacaug gcuagaauca agagaauaua caaaacauuu aauaagagua      420

gaaaacugga uuuuuagaaa uccaggauuu gcauuagcug cugcagcuau agcauggcuu      480

cuuggcaguu caaccagcca aaaaguuaua uauuugguca ugauuuugcu aauagcacca      540

gcauauagua uacgauguau aggaguauca aauagagacu uuguugaagg aaugucugga      600

ggaacauggg ucgauguagu acuagaacau ggaggaugug uaacaguaau ggcacaagau      660

aaaccaacag uugauauaga auuaguaaca acaacaguau cgaauauggc cgaaguaaga      720

aguuauuguu augaagcauc aauauccgau auggcaucag auagcagaug uccaacucaa      780

ggagaagcau accuagauaa acaaucagau acucaauaug uuuguaaaag aacauuaguu      840

gacagaggcu gggguaaugg augcgguuua uucggcaaag gcucauuagu aacaugugca      900

aaguuugcau guucaaagaa aaugacagga aaaucaauac aaccagaaaa uuuagaauau      960

cgaauaaugu uaaguguaca uggaucccag cacucaggaa ugauaguuaa ugauacaggu     1020

cacgaaaccg augagaauag agcaaaagua gagauaacuc caaauagucc aagagcagaa     1080

gcuacauuag gaggauuugg uagcuuaggu uuagauugug aaccacgaac aggauuagau     1140

uucagugauc uauauuaucu aacuaugaau aauaaacacu gguuaguaca uaaagaaugg     1200

uuucaugaua ucccacuucc auggcaugca ggagcagaua caggaacacc ccauuggaau     1260

aauaaagaag cauuggucga auuuaaagau gcacaugcaa aaaggcaaac aguuguagug     1320

uuaggaucac aagaaggagc uguacacaca gcacuugcug gugcacuuga ggcagaaaug     1380

gauggagcaa aaggaagacu gagcagcggg cauuuaaaau guagauugaa aauggauaaa     1440

uuacgccuga aaggaguaag uuauagucua uguaccgcag cauuuacauu uacaaaaaua     1500

ccagcugaaa cgcuacaugg aacggucaca guagaaguac aauaugcagg uacagaugga     1560

ccauguaaag uaccagcaca aauggcagua gacaugcaaa cacuaacacc aguuggaaga     1620

uugaucacug caaauccagu uauaacagaa ucaacugaaa auucgaaaau gauguuagag     1680

uuagacccuc cguuugguga cucauauaua guaaucggag uaggagaaaa gaaaauaaca     1740

caucacuggc auagaucagg aaguacacua ggaaaggcuu uuagcacaac auuaaaaggu     1800

gcucaaagau uagcagcauu aggcgauaca gcuugggacu uuggaucaau aggcggagua     1860

uuuaacagua uaggaagggc cguacaucaa guauuuggag gagcauuuag aacacucuuu     1920

ggaggaauga gcuggauaac acaagguuua augggagcau uauuacuuug gaugggagua     1980

aaugcacgag aucgaaguau agcauuagca uuucuagcaa cugguggagu ccuaguauuu     2040

cuagcaacaa acguacaugc u                                               2061


<210>  2486
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       CDS opt

<400>  2486
acaucaauag guaucauagg ccuauuacua acuacagcaa uggcagcaga aauaacuaga       60

agagguucag cauauuacau guaucuagau agaagugaug cagguaaagc aauaaguuuu      120

gcaacaacau uaggaguaaa uaaaugccau guacaaauaa uggaucuagg ucauaugugu      180

gacgcaacaa ugaguuauga augcccuaug cuagacgaag gaguugaacc ugaugacgua      240

gauuguuggu gcaacacaac aaguacuugg gugguauaug gaacuuguca ucacaagaaa      300

ggagaagcaa gacgaucaag acgagccgua acucuaccau cucacucaac aagaaaacua      360

caaacacgcu cacagacaug guuagaauca agagaauaca cuaaacauuu aauuaaaguu      420

gaaaacugga uuuuucgaaa uccagguuuu gcauuaguag cuguagccau agcauggcua      480

cuaggaaguu cuacaucuca aaaaguaaua uaucuuguaa ugauacuacu aauagcucca      540

gcauauucaa uuagauguau aggaguaagc aauagagauu ucguagaagg aaugagugga      600

ggaacauggg uagauguagu auuagaacau ggugguugug uaacaguaau ggcacaagac      660

aaaccaacag ucgacauaga auuaguuaca acuacgguaa guaacauggc agaaguuagg      720

aguuauuguu augaggcauc aauaagcgau auggcgucag auucaagaug cccaacacaa      780

ggagaagcau auuuagacaa acaaagugau acgcaauacg uauguaaaag aacucuagua      840

gacagggguu ggggaaaugg augcgguuua uuuggaaaag gcagucuagu aacaugcgca      900

aaauuuacau guucaaagaa aaugacagga aaaucuauuc aaccugagaa uuuagaauau      960

cgaauaaugu uaucuguaca uggaucucaa cauucaggaa ugauagggua ugaaacugac     1020

gaagauagag caaaaguaga aguaacgccc aauucuccaa gagcagaagc aaccuuaggc     1080

ggauuuggaa guuuaggacu agauugugaa ccaagaacag gucuagacuu uucagaucuc     1140

uacuaucuaa caaugaauaa uaaacacugg cuugugcaua aggaaugguu ucaugauauc     1200

ccauuaccau ggcaugcagg agcagacaca ggaacaccuc auuggaauaa uaaagaggcc     1260

uuaguagaau uuaaagaugc acaugcgaaa agacaaacag uaguaguuuu aggaagucaa     1320

gagggagcag uucauacagc auuagccgga gcacuugaag cggaaaugga uggagccaaa     1380

ggacgucuau uuaguggaca cuuaaaaugc agauuaaaaa uggauaaacu uagacuaaaa     1440

ggugucucau acucucuaug cacagccgcg uuuacauuua ccaagguacc agccgaaaca     1500

cuucacggaa ccguaacugu agaaguacaa uaugcaggaa cagauggacc auguaaaauu     1560

ccuguacaaa uggcaguaga uaugcaaaca uuaacacccg uagguagauu aauaacagca     1620

aaucccguaa uuacagaauc aacagaaaau aguaaaauga uguuagaacu agauccucca     1680

uuuggagauu cauauauagu aauaggagua ggugacaaga aaauaacaca ucauuggcac     1740

agaaguggua gcacauuagg aaaagcauuu aguacaacac ucaaaggagc acaaagauua     1800

gcagcacuag gagauacagc augggacuuu ggaagcauug gagguguauu uaauaguauc     1860

gguagagcag uucaucaagu guuuggagga gcauucagaa cacuauuugg agguaugucu     1920

uggauuacac aagguuuaau gggugcauua uuauugugga ugggaguaaa ugcacgagau     1980

cguucaauag cauuggccuu uuuagcaacc gguggagucc ugguguuuuu agcaacuaau     2040

guccaugca                                                             2049


<210>  2487
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2487
acaucuguag guauaguagg acuccuuuua acaacagcaa uggcagcaga aguaacgaga       60

agagguagcg cauauuauau guauuuagau agaaacgacg ccggagaagc aauuagcuuu      120

ccuacaacau uaggcaugaa uaaauguuau auacaaauaa uggaccuugg acauaugugu      180

gaugcaacua ugagcuauga augcccuaug cuagaugagg gaguagaacc agaugacgua      240

gauuguuggu guaacacaac aagcacaugg gucguuuaug gcacaugcca ucauaaaaaa      300

ggagaggcaa gacguagucg aagagcagua acacuaccau cgcauucaac acgaaaauua      360

caaacuagaa gccaaacaug gcuagaauca cgagaguaca caaaacauuu aauaagagua      420

gaaaacugga ucuuuaggaa uccugguuuu gcacuagcag cagcagcaau cgcauggcua      480

cucggaagua guacgaguca aaaaguaauc uaucuaguaa ugauuuuacu aauagcaccc      540

gcuuacucaa uaagguguau agguguauca aauagagauu uuguggaagg uaugaguggu      600

ggaacauggg uugauguagu acuagaacau ggaggaugug ucacaguuau ggcucaagau      660

aaaccaaccg uugauauaga guuaguuacu acuaccguuu ccaauauggc agaaguaaga      720

agcuacugcu augaagcaag caucucagau auggcaucag auucaagaug cccaacccag      780

ggagaagcau auuuagacaa acaaucagau acacaauaug uaugcaaaag gacauugguu      840

gauagaggau gggguaaugg augcggauua uuuggcaaag gaucucuagu aaccugugcu      900

aaauucgcau gcucuaaaaa aaugacagga aaaucgauuc aaccggaaaa ucuugaauac      960

cgaauaaugc uuucaguaca uggaagccaa cauucaggaa ugaucguaaa cgauacaggu     1020

cacgaaacag augagaauag agcuaaagua gaaauaacuc caaauagccc acgugcugaa     1080

gcuaccuuag gaggauuugg gucuuuagga uuagauugcg aaccacgaac aggauuagau     1140

uucucagacc ucuauuaucu cacaaugaac aauaaacauu gguuagucca uaaagaaugg     1200

uuccaugaca ucccacuacc auggcacgca ggugcggaca cuggaacacc gcacuggaac     1260

aacaaagaag cacuagucga auucaaggau gcucacgcaa aaagacaaac aguaguagua     1320

cugggaucac aagaaggagc aguacacaca gcuuuagcag gagcauuaga agcagaaaug     1380

gacggagcaa aaggaagauu aagcagcgga caucuuaaau guagauuaaa aauggauaaa     1440

uugagauuaa aaggagucuc guauucauua ugcacagccg cauuuacauu uacaaaaaua     1500

cccgccgaaa cacuacaugg aacaguuacu gucgaaguac aauaugcagg gacagacggu     1560

ccauguaaag uaccugcaca aauggcaguu gacaugcaaa cacuaacacc aguaggaaga     1620

uuaaucacag caaacccagu aauaacagaa ucuacagaaa acaguaaaau gauguuagaa     1680

uuagauccac cuuuuggcga uuccuauaua guaauuggag uaggagaaaa aaaaauuaca     1740

caucauuggc auagaagugg uucaacacua ggcaaagcuu uuucaaccac acuaaaaggu     1800

gcucagcgac uagcagcacu uggagauaca gcaugggauu ucggaaguau cggaggaguc     1860

uuuaacucaa uaggucgagc aguucaucaa guauucggcg gagcauuuag aacacuauuu     1920

gguggaaugu cauggauaac acaaggacua augggagccu uauuauuaug gaugggagua     1980

aaugcgagag auagaaguau ugcauuagca uuuuuagcaa cugguggagu acuaguauuu     2040

uuagcaacua acguacaugc g                                               2061


<210>  2488
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  2488
agaauuauaa acgcucgaaa agaaaaaaaa agacgaggag cagauacaag uguugguaua       60

guagguuuau uguuaacuac ugcaauggca gcagaaguaa caagacgagg aagcgcuuau      120

uauauguauc uagacagaaa ugaugcagga gaagcgauaa guuucccaac uacauuaggu      180

augaauaaau guuacauaca aauaauggau uuagggcaua ugugugaugc aacaaugagu      240

uaugaaugcc caauguuaga ugagggagua gaacccgaug acguagauug cuggugcaac      300

acuacaagca cauggguagu auaugguaca ugucaucaua aaaaaggcga agcaagaaga      360

agccgaagag caguuacacu accaucacau ucaacaagaa aacuucaaac gcgaucacag      420

acuuggcuag aaucaagaga auauacaaaa caucuaauua ggguagaaaa uuggaucuuc      480

agaaacccag gauuugcgcu agcagcugcu gcuauugcuu gguuauuagg aucaucaaca      540

agccaaaaag uaauuuaucu ugugaugauu cuacuaauug cacccgcaua uucuauacgc      600

ugcauaggag ugucgaaccg agauuuuguc gaaggaaugu ccgggggaac cuggguagau      660

aucguauuag aacauggagg uuguguuacu gugauggcac aagauaaacc aacaguagau      720

auagaauuag uuacaacaac aguaagcaau auggcagaag uuagauccua cuguuaugaa      780

gcauccauua gcgauauggc uagugauagc agauguccaa cacaaggaga agcuuacuua      840

gauaaacaaa gugacacaca auauguaugc aaaaggacau ugguagaccg aggauggggc      900

aauggaugcg guuuauucgg uaaaggcuca uuaguaacuu gugcaaaauu ugcguguuca      960

aaaaaaauga caggaaaaag uauacaacca gaaaauuuag aauacagaau aauguuauca     1020

guacacggau cccaacacuc aggaaugaua guaaaugaca caggacauga aacagaugaa     1080

aacagagcaa aaguagagau aacaccaaau ucaccaagag cugaagccac auuaggagga     1140

uucggaaguu uagguuuaga cugugaacca agaacuggau uagauuuuag cgacuuauau     1200

uauuuaacaa ugaacaauaa acauugguua guacauaaag aaugguuuca ugauauacca     1260

uuacccuggc augcaggagc ugauacaggg acuccccauu ggaacaauaa agaagcacuu     1320

guagaauuca aggaugcaca ugcgaaaaga caaacaguag uagucuuagg cagccaagaa     1380

ggggcaguuc acacagcauu agcaggagcu uuagaagcgg agauggaugg cgcaaaagga     1440

agauuaagcu caggccacuu aaaaugcagg uuaaagaugg acaaauuaag auuaaaagga     1500

guaaguuaua gccuaugcac agcagcauuu acauucacua aaauaccagc cgaaacauua     1560

cacggaaccg uuacuguuga aguacaauac gcaggaacag acggacccug caaaguacca     1620

gcucaaaugg ccguagauau gcaaacauua acucccguug ggagacuaau aacagccaau     1680

ccaguaauaa cagaaaguac ugaaaauuca aaaaugaugu uagaauuaga uccaccuuuu     1740

ggagacucau acauaguuau agguguaggg gaaaagaaaa uaacacacca cuggcacaga     1800

ucaggcucaa cuauugguaa agcauuugaa gcuacaguca gaggcgcgaa aagaauggca     1860

guacuaggag auacagcaug ggauuucgga ucaguuggag gggcauuaaa caguuuagga     1920

aaaggaauac aucaaaucuu cggagcagcu uucaaaucuu uauuuggagg uaugucuugg     1980

uuuucucaga uacuaauagg uacauuauua augugguuag gacuaaauac aaaaaauggg     2040

ucaaucucuu ugauguguuu agcacuaggu ggaguguuga uauuuuuauc aacagcagua     2100

agugcagacg uagguuguuc aguagauuuu ucaaaa                               2136


<210>  2489
<211>  2133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2489
auaauuaacg cuaggaaaga aaaaaaacga agaggggcag auacauccgu aggcauagua       60

gguuuguuau uaacaacagc cauggcagca gaaguaacac gaagaggcuc agcauacuac      120

auguauuuag auagaaauga ugcaggagaa gcaauaagcu uuccaacuac auuaggaaug      180

aauaaauguu auauacaaau cauggaucua ggacacaugu gugaugcgac aaugucauau      240

gaaugcccaa uguuggauga aggaguugaa ccagaugaug ucgauugcug guguaauaca      300

acaaguacau ggguaguaua uggaacaugc caucauaaaa aaggagaagc cagaagaagc      360

agacgcgcag uaacacuccc aucucauagu acaagaaaac uacaaacaag aucucaaacc      420

uggcuagaga gcagagaaua cacaaaacau uuaauaagag uagagaauug gauuuucaga      480

aauccuggau uugcccuggc agcagcagcu auugcauggc uacuugguuc gagcacgagc      540

caaaaaguaa ucuaucucgu uaugauacua cuaauagcuc cagcuuacag cauaagaugu      600

auuggaguau caaauagaga uuuuguagaa gguaugucag gaggaacaug gguagauauc      660

guuuuagaac auggaggaug uguaacagua auggcgcaag acaaaccaac gguagacauc      720

gaauuaguaa caacgacagu aaguaauaug gcagaaguaa gaaguuacug cuacgaagca      780

aguauuucag auauggcauc agacucacga uguccaacac aaggagaagc auauuuggau      840

aaacaaucag acacacaaua uguaugcaaa agaacacuag uagacagagg augggggaac      900

ggauguggac uuuuuggaaa agguucuuua guaacaugug caaaauuugc gugcucaaaa      960

aagaugacag gaaaaaguau acaaccagaa aaucuugaau auagaauaau guuaucagua     1020

cacgggagcc agcauagcgg caugauaguu aacgacacug gacacgaaac agacgaaaac     1080

agagcuaaag uagaaauaac cccaaauagu cccagggcag aagcaacguu aggaggauuu     1140

ggaaguuuag gacuagacug ugaaccacga acaggacuag auuuuagcga ucuauacuac     1200

uuaacaauga acaacaaaca cuggcuaguc cauaaagaau gguuucauga cauaccguua     1260

ccauggcaug caggagccga caccggaacu ccacauugga auaacaaaga ggcauuagua     1320

gaauuuaaag augcacaugc aaaaagacag acuguaguag uacuaggaag ucaagaagga     1380

gcuguacaca cagcacuugc aggagcauua gaagcagaaa uggacggagc aaaagggcgg     1440

uugucauccg gacaucuaaa augucgauua aaaauggaca aauuaaggcu uaaaggagua     1500

ucuuacaguc uaugcacagc agcauuuaca uuuacaaaaa uaccagcaga aacauuacau     1560

gguacaguaa caguugaagu acaauaugca ggaacagaug gcccauguaa aguuccagca     1620

cagauggcag uagauaugca aacuuuaacc ccaguaggaa gauugauaac agcuaauccc     1680

guaauaacag aaaguacaga aaauucaaaa augauguuag aacuggaucc accauuugga     1740

gauagcuaua uaguaauagg aguaggagag aaaaagauaa cacaucauug gcacagaagu     1800

ggaagcacaa ucggaaaagc uuuugaagca acgguaaggg gcgcaaaaag aauggcagua     1860

uuaggagaca ccgcguggga uuuuggaagc guaggaggag cauuaaauag uuuaggaaaa     1920

ggaauacauc aaaucuucgg agcugcauuc aaaucauuau uuggugguau gucaugguuu     1980

ucacaaauac uaauagguac ccuucuaaug ugguuaggau uaaauaccaa aaacggcagc     2040

auaagcuuaa uguguuuagc auuggguggu guuuuaauuu uuuuaucuac agcaguuuca     2100

gcagaugucg guuguagcgu ggauuuuagc aag                                  2133


<210>  2490
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2490
auaaaugcaa gaaaagaaaa aaaacgaaga ggagcagaca caagugucgg aauaguaggu       60

uuauuauuaa cuaccgcaau ggcggcagaa guaacgagaa gaggaucugc auauuacaug      120

uauuuagauc guaaugaugc uggagaagca auaucuuucc caacaacacu aggaaugaau      180

aaaugcuaua uucaaauaau ggaccuagga cauaugugcg augcaacaau gaguuaugaa      240

uguccaaugu uagacgaagg aguagaacca gacgaugugg acuguuggug uaauacaacu      300

agcacguggg uagucuacgg aacaugccau cauaaaaaag gggaagcaag aagaaguaga      360

cgagcaguaa cguuaccauc acauagcacc agaaaauuac aaaccagguc acaaacuugg      420

cuagaaucac gagaauacac aaaacaucua auuagaguag aaaacuggau auuucgaaac      480

ccgggauuug ccuuagcugc agcagcaaua gcauggcuau uaggaucaag uacuucacaa      540

aaaguaauuu aucuaguaau gauacuauua auagcgccag cauauucaau aagguguauu      600

ggaguuagca auagagauuu uguagaagga augaguggag gaacuugggu cgauaucgua      660

cuagaacaug gaggaugugu aacaguaaug gcacaagaca aaccaacagu agauauagaa      720

cuaguuacaa caacaguaag uaauauggcc gaagugagaa gcuauuguua ugaagccuca      780

auaucagaua uggcaaguga cucaaggugu ccaacucaag gagaagcaua ucuggauaag      840

caaucagaca cacaauaugu cuguaaaaga acguuaguag auagagguug gggaaaugga      900

uguggucucu ucggaaaagg aucauuagua acaugugcaa aauuugccug cagcaaaaaa      960

augacaggaa aaagcauaca accugaaaac cuggaauaua gaauaaugcu cucaguacau     1020

gguagccaac auucagguau gauagugaau gauacaggac augaaacuga ugaaaaucga     1080

gcaaaaguag aaaucacgcc uaauaguccg agagcugaag cuacacuagg aggauuugga     1140

aguuuaggau uagacuguga accaagaaca ggauuagauu uuagugaccu cuacuauuua     1200

acaaugaaua auaaacauug gcucguacau aaagaguggu uucacgacau accauuacca     1260

uggcacgcag gugccgauac aggaacuccg cauuggaaua acaaagaagc auugguugaa     1320

uuuaaagaug cacaugcaaa gagacaaaca guaguuguuu uaggaagcca agaaggagca     1380

guccacacag cuuuagcagg agcuuuagag gcagaaaugg auggagcaaa aggaagacuu     1440

agcucuggcc accuaaaaug cagauugaaa auggauaaac ugagacuaaa aggaguaucu     1500

uauagucuau guacagccgc auucacauuu acuaaaauuc cagcagaaac uuuacauggu     1560

acaguaacug uagaaguuca auaugcagga acagauggcc cuuguaaagu accugcccaa     1620

auggccguag auaugcaaac auuaacacca guaggaagau uaauaacagc aaauccagua     1680

auaacagaaa gcaccgaaaa uagcaaaaug auguuagaau uagauccacc auuuggugau     1740

aguuauauag uaauuggagu aggcgaaaaa aaaauaacac aucacuggca uagaucaggg     1800

ucaacaauag guaaagcauu ugaagccaca guaagaggag caaaaagaau ggcaguuuua     1860

ggagauaccg ccugggacuu ugguucagua ggaggagcuc uuaauucuuu aggaaaagga     1920

auacaccaaa uauuuggcgc agcauuuaag aguuuauucg gaggaauguc augguuuuca     1980

caaauucuaa uaggaacguu auuaaugugg cuagguuuaa auacaaaaaa ugggagcauc     2040

ucccuaaugu gucuugcauu aggaggagua cuuauauuuu uaagcacugc aguaagugcg     2100

gacguagguu guaguguaga uuuuagcaaa                                      2130


<210>  2491
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2491
aacgcuagaa aagaaaaaaa acguagaggu gccgacacaa gcguaggaau uguaggguua       60

uuauuaacaa cagcaauggc agcugaagua acuagaagag guucagcaua cuauauguac      120

uuagauagaa augacgcugg agaagcaaua aguuuuccaa caacauuagg uaugaauaaa      180

uguuauauac aaauaaugga ucuuggucau augugcgaug ccacaauguc guacgaaugu      240

ccaaugcuag augaaggagu cgaaccagau gauguagauu guugguguaa cacaacaagc      300

acuuggguag uguaugguac augucaucac aaaaaaggag aggcaagaag aaguagacgg      360

gcuguuacac uucccagcca cucuacgaga aaacuacaaa cacgaaguca aacauggcua      420

gaaucaagag aauacacaaa acaccuaaua agaguagaaa auuggauauu uagaaaucca      480

ggguucgcuc uugcagcagc agcaauagca uggcuauuag gaaguaguac uagucaaaaa      540

gugauauauc uaguaaugau acuuuuaaua gcgccagccu acagcauaag auguaucgga      600

guaaguaaua gagauuuugu cgaaggaaug ucuggaggua cuuggguaga cauaguacua      660

gaacacggug gaugcgugac uguaauggca caggacaaac ccacaguaga cauugaauua      720

guaacaacaa caguaucaaa cauggcagaa guaagaucau auuguuauga agcaaguaua      780

agugauaugg caucagauag ucgaugucca acacaaggag aagcuuauuu agauaaacaa      840

agugauacgc aauacguaug uaaaagaacg cuaguagaca gggguugggg aaacggaugu      900

gguuuauucg guaaggguuc auuaguaaca ugugccaaau uugcauguag uaaaaaaaug      960

acuggaaaau cuauacaacc ugaaaauuua gaauacagaa uuauguuauc gguucaugga     1020

agccagcaca gcggaaugau aguuaaugau acaggacaug aaacggauga aaauagagca     1080

aaaguagaaa uaacaccaaa caguccaaga gcagaagcaa cauuaggagg auuuggaagc     1140

uuaggauugg auugugagcc gagaacagga uuagauuuua gcgaucuaua cuauuuaaca     1200

augaauaaca aacauugguu aguacauaaa gaaugguuuc acgauauucc acuaccgugg     1260

caugcaggag cagauacagg uacaccgcau uggaauaaca aagaggccuu aguugaguuc     1320

aaagaugcuc augcuaaaag acaaacgguc guaguacuag gaucacaaga aggagcagua     1380

cacacagcac uagcaggagc ucuugaagcc gaaauggaug gugcgaaagg acgauuauca     1440

aguggacauu uaaaaugcag acuaaaaaug gauaaauuga gauuaaaagg aguaaguuac     1500

agcuuaugua cagcugccuu uacuuucaca aaaaucccag cugaaacauu acauggaaca     1560

guuacaguug aaguucaaua ugcaggaaca gaugguccau guaaaguacc agcacaaaug     1620

gcaguagaua ugcaaacauu aacuccagua ggaagauuaa uaacugccaa uccuguaauc     1680

acagaaagua ccgaaaauuc aaaaaugaug uuagaauugg auccgccauu uggagauucu     1740

uauauaguca uagguguagg ugaaaaaaaa auaacacauc auuggcauag aagugguuca     1800

acaauaggua aagcauucga agcaacagua aggggagcaa aaagaauggc aguacuagga     1860

gauacagcuu gggacuuugg uagcgucgga ggugcuuuaa auagcuuagg aaaagguauu     1920

caucaaauau uuggugccgc auuuaaaagu uuauucggag ggaugaguug guuuagucaa     1980

auacucauug gaacucuuuu gauguggcua ggauuaaaca caaaaaaugg aaguauuuca     2040

cuaauguguu uagcauuagg aggaguauua auauuccuau cuacagcagu cucagcugau     2100

guaggaugcu cagucgacuu uaguaaa                                         2127


<210>  2492
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2492
gcaagaaaag aaaagaaaag gagaggagca gacacaagug uaggaauagu agguuuauua       60

cugacuacgg caauggcagc agaaguuaca agaaggggaa gcgcauauua uauguauuua      120

gauagaaaug augcaggaga agcaauauca uucccaacca cauuaggaau gaacaaaugu      180

uacauacaaa uaauggaccu cggacauaug ugcgaugcca caaugucaua ugaaugcccg      240

auguuagacg aaggaguaga accagaugau guagauuguu gguguaauac uacuagcaca      300

uggguaguau acggaacaug ccaucauaag aaaggagaag cucgaagauc uagaagggcu      360

guaacuuuac ccucacauuc aacacguaaa uuacagacaa gaagccaaac auggcuagaa      420

ucaagggaau auacaaaaca cuuaauuaga guagaaaauu ggauauucag aaauccagga      480

uucgcguuag cagccgcagc gauagcuugg uuauuaggau caaguacauc ucaaaaagua      540

auauaucuag ucaugauacu acuaauagcg ccagcuuaca guauaagaug cauaggagua      600

aguaacagag acuuuguaga aggaauguca ggaggcacau ggguagacau aguacuagaa      660

caugguggau guguaacagu gauggcacaa gacaaaccaa caguagauau agaauuagua      720

acaacgacag uaagcaauau ggcagaagua aggaguuauu guuaugaagc aucaauuuca      780

gauauggcaa gcgauucaag augucccacu caaggugaag cauaucuaga uaaacaaagc      840

gauacgcaau auguauguaa aagaacuuua guagaucgag gcugggguaa cggaugcggu      900

uuauuuggaa aagggagcuu agucacaugu gcaaaauucg cauguucgaa gaaaaugacu      960

ggaaaaagua uacaaccuga aaaucuagaa uacagaauua uguuaucagu acauggaagu     1020

caacauagug gaaugauugu aaacgauaca ggucaugaaa cggacgaaaa ucgcgcaaaa     1080

guagaaauaa caccuaauag uccacgagca gaagcuacuu uaggggguuu cgggucauua     1140

ggauuggacu gugaaccaag aacagguuua gacuuuagug accuauauua cuuaacaaug     1200

aacaauaaac acugguuggu acacaaagaa ugguuucaug acauaccauu acccuggcau     1260

gcaggagcag acacuggaac uccacauugg aauaauaaag aagcgcuagu agaauuuaaa     1320

gaugcacaug caaaaagaca aacuguaguu guccuaggau cacaagaagg agcaguacau     1380

acagcauuag cuggagcauu agaggcagaa auggauggag caaaaggaag auuauccagu     1440

gggcaucuaa aaugcagguu aaaaauggau aaacuuaggc uaaaaggagu aucuuacuca     1500

cuauguaccg cugcauucac auuuacaaaa auuccugcag aaacauuaca uggaacagug     1560

acaguagaag ugcaauaugc uggaacugac gguccaugua aaguuccagc acaaauggca     1620

guagauaugc aaacauuaac uccuguaggu agacuuauaa cagcaaaccc aguaauaaca     1680

gaaaguacag aaaauucaaa aaugaugcua gaauuagauc caccauucgg agauagcuau     1740

auagugauug gaguagguga aaagaaaauu acacaccacu ggcacaggag ugguaguaca     1800

auaggaaaag cguuugaagc aacuguaaga ggugcaaaaa gaauggcugu cuuaggugac     1860

acugcauggg auuuugguag uguuggagga gcuuuaaauu cacuaggaaa agguauacac     1920

caaauauucg gagcagcauu uaaaagucua uuuggcggua uguccugguu uucacaaaua     1980

uuaauaggaa cguuauuaau gugguuagga cuaaacacga aaaauggaag cauaucauug     2040

auguguuuag cucuaggagg aguuuuaaua uuucuuucaa cagcagucuc agcagauguu     2100

ggauguucag uagacuuuag uaaa                                            2124


<210>  2493
<211>  2121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2493
agaaaagaaa aaaaaagacg aggagcagac acaucagucg gaauuguagg acuacuuuua       60

acaacagcaa uggcagcaga gguuacuagg agagguucag ccuauuauau guauuuagau      120

agaaacgaug cuggcgaagc aauuaguuuc cccacaacau uaggaaugaa uaaauguuau      180

auacaaauua uggauuuggg ucauaugugc gacgcaacaa ugaguuauga augccccaug      240

cuggaugaag gggucgaacc agacgaugua gauuguuggu guaacacaac aucaaccugg      300

guaguauacg ggacauguca ucacaaaaaa ggagaagcga gacgaagcag aagagcaguu      360

acacuaccaa gucacucaac acgaaaacua caaacacgau cacaaacaug guuagaaagu      420

agagaguaca caaaacaucu uauucgagua gagaauugga uauuuaggaa uccaggauuu      480

gcauuagcag cagcagcaau agcaugguua uuagguucau caacaaguca aaaaguaaua      540

uaccuuguaa ugauacuacu uauagcaccu gcuuauagua uaagauguau uggaguauca      600

aauagagacu uugucgaagg aaugagugga ggcacguggg uagauauagu auuagaacac      660

ggaggaugug uaacagucau ggcacaagac aaaccuacag uagacaucga acucgucacu      720

accacaguau caaauauggc agaaguaaga ucauacuguu augaagccag uauaagugau      780

auggcaagug acucaagaug uccaacacaa ggugaagcau aucucgauaa acaauccgau      840

acucaauaug uauguaaaag aacauuagua gauagaggau ggggcaaugg auguggacuu      900

uuuggaaaag ggucacuagu aacaugcgca aaauuugcau guaguaaaaa aaugacaggu      960

aaaucaauac aaccagaaaa ccuugaauau cgaauaaugu uaaguguuca uggaagccaa     1020

cacucgggca ugaucgugaa cgacacaggg cacgaaaccg augagaauag agcaaaggua     1080

gaaauaacac caaauagccc aagagcagaa gcuaccuuag gaggauucgg aucauuaggc     1140

uuagauugug aaccaagaac aggguuagac uuuucagauc uauacuaucu aaccaugaau     1200

aauaaacauu ggcuaguuca caaagaaugg uuucaugaua uaccauuacc auggcacgca     1260

ggagcagaca cgggaacacc acauuggaac aacaaagaag cauuagugga auuuaaagau     1320

gcacacgcaa aaagacaaac uguuguggua cuaggaaguc aggaaggagc aguacauaca     1380

gcauuagcgg gagcgcuaga agcagaaaug gauggagcaa aaggaagacu aucaucuggu     1440

cacuuaaaau guagauugaa aauggauaag uuaagauuaa aggggguuag cuauucucua     1500

uguacagcgg ccuucacuuu uacaaaaauu ccggcagaaa cacugcaugg aacaguaaca     1560

guagaagucc aguaugcagg uacagacgga ccauguaaag uacccgccca aauggcagua     1620

gauaugcaaa cauugacucc aguaggaaga cucauaaccg cuaauccggu uauaacagag     1680

aguacagaaa acucaaaaau gaugcuagaa cucgauccgc cauucggaga uucguauaua     1740

guuauaggug uuggagaaaa aaaaauaaca caccauuggc auagaucagg cuccacaaua     1800

ggaaaagccu uugaagcuac cguaagggga gcaaaaagaa uggcaguauu aggagauacu     1860

gcaugggauu ucggaucagu uggaggagca cuaaauagcu uaggaaaagg aauacaucaa     1920

auauuuggag cagcauuuaa aucuuuguuu ggaggaaugu caugguucag ucaaauauua     1980

auaggaacgu uauuaaugug guuaggucug aauaccaaaa auggaagcau uaguuuaaug     2040

uguuuagcau uaggaggagu auuaauauuu cuaucgacag cuguaucagc agaugugggu     2100

uguucaguag auuuuuccaa a                                               2121


<210>  2494
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2494
aaagaaaaaa aaagaagagg agcagauaca ucaguaggaa ucguaggauu acuauugaca       60

acagcuaugg cagcagaagu aacaagaaga ggaucggcau auuauaugua uuuagaucgc      120

aacgaugcug gagaagcaau uucauuccca accacuuugg gaaugaauaa augcuacaua      180

caaauaaugg auuugggaca uaugugugac gcgacuaugu cauacgagug uccgaugcua      240

gaugaaggag uagaaccuga ugacguagau uguuggugua auacuacaag cacuugggua      300

gucuauggua cgugccauca uaaaaaaggu gaagcucgaa gaagcagaag agcaguuacu      360

uuaccuucac acucaacaag aaaauuacaa acgagaaguc aaacaugguu agaaucaaga      420

gaauauacaa aacauuuaau aagaguugaa aauuggauau uuagaaaucc ugguuuugca      480

uuagcugcgg cagcaauagc cugguuauua ggaucaucaa cuagucaaaa aguaauuuau      540

uuaguaauga uuuuauuaau agcaccugcg uauaguauaa gauguauagg aguaaguaau      600

agagauuuug uagaagguau gucaggagga acauggguag acauaguauu ggaacauggg      660

gggugcguaa cagucauggc ucaagacaaa ccaacaguag acauagaauu aguuacaaca      720

acuguaucca auauggcaga aguaagaagc uauuguuacg aagcuucuau aagcgacaug      780

gcuucagauu caagaugucc uacacaaggu gaagcauauu uagacaaaca aagugauacc      840

caauacguau gcaaaagaac auugguggau agaggauggg guaauggaug cggauuauuu      900

ggaaagggau cacuaguaac augugcaaaa uuugcaugua gcaaaaaaau gacaggaaaa      960

aguauacaac cggaaaaucu agaauauaga auaauguuau cgguacacgg uagucaacau     1020

ucaggaauga uaguaaauga uacaggacau gaaacagaug agaacagagc uaaaguugaa     1080

auuacaccga auucuccaag agcagaagca acgcuaggag gauuuggaag ucuagguuua     1140

gauugcgaac caagaacagg uuuagacuuc agugacuuau acuacuuaac aaugaacaau     1200

aaacauuggu uaguacacaa agaaugguuu caugauauac cacuaccaug gcaugcagga     1260

gcggauacug gaacaccaca cuggaacaau aaagaagcau uaguagaauu uaaagaugca     1320

caugcaaaaa gacaaacagu aguagugcuc gguucacaag aaggagcagu gcacacagca     1380

uuggccggag cacuagaagc agaaauggau ggagcaaaag gaagacuauc aucaggacau     1440

cuaaaaugca ggcuaaaaau ggauaagcuu agauuaaaag gaguaucaua uucacugugc     1500

acagcagcuu ucacauucac caaaauuccu gcagaaaccu ugcacgguac cguaacagua     1560

gaaguacagu augccgggac agauggacca uguaaaguac cagcgcaaau ggcggucgau     1620

augcagacuc uaacaccagu gggaagguua auaacagcua aucccgugau aacagaauca     1680

acagaaaaua guaaaaugau guuagaacua gauccuccau uuggagauuc auacauagua     1740

auaggaguug gagaaaaaaa aauaacacau cacuggcaua ggagcggcuc aaccauugga     1800

aaagcauucg aagcuacagu aagaggcgca aaacguaugg caguauuagg agauacagca     1860

ugggauuuug gaucaguugg aggugcauua aacucacucg gaaaaggaau acaucaaaua     1920

uucggagcug cuuucaaauc cuuguucgga gguaugaguu gguuuucuca aauauuaaua     1980

ggaacacuau ugaugugguu aggauuaaau acaaaaaacg gaagcauauc auuaaugugu     2040

uuagcauuag ggggugucuu aauuuucuua ucaacagcag ugucagcaga ugucgggugu     2100

agcguugauu uuaguaaa                                                   2118


<210>  2495
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2495
gaaaagaaaa gaagaggagc agauacauca gugggaauug uagggcuauu auuaacaacc       60

gcaauggcag cagaaguaac aagaagggga agcgcauacu auauguauuu agaccguaau      120

gacgcuggag aagcgauuuc cuuucccaca acauuaggaa ugaauaaaug cuacauacaa      180

auaauggacu uaggacauau gugugaugcc acaaugucau acgaaugucc aaugcuugac      240

gaaggaguag aaccagauga cgucgacugu uggugcaaua caacaucuac uuggguuguu      300

uaugggacuu gucaucacaa gaaaggagag gccagacgaa gucguagagc aguaacuuua      360

ccaagccacu caacaagaaa acuacagacu agaagucaaa cgugguuaga aucaagagaa      420

uauacaaaac auuuaauacg aguggaaaac uggauauuca gaaacccugg auucgcauug      480

gcagcagcug caauugcaug guuauuagga ucaaguacau cacaaaaagu aauauauuua      540

guuaugauau uacuaauugc acccgcuuau ucaauaagau guauaggagu aucuaacaga      600

gauuuuguug aaggcaugag cggaggaaca uggguagaua uaguauugga acauggggga      660

ugcguuacag uaauggcaca agauaaacca acaguggaua uagaauuagu cacaacaaca      720

guuaguaaca uggcagaggu acgaucuuau uguuacgaag caaguauaag ugauauggca      780

ucggauagua gauguccuac acaaggagag gcuuauuuag acaaacaaag ugauacacaa      840

uauguuugua aaagaacacu uguugauaga gguuggggua acgguugcgg auuauuuggu      900

aaaggcucac ugguuacgug ugcaaaauuc gcauguagca agaaaaugac uggaaaaagu      960

auacaaccag aaaaucuaga auauagaaua auguuaagug uacacggaag ccaacauuca     1020

ggaaugauag uaaaugacac aggacacgaa acagacgaaa auagagcaaa aguugaaauc     1080

acuccuaauu caccaagagc agaagcaaca cuaggagguu uuggaucucu aggccucgac     1140

ugugaaccac gcacaggauu agacuuuuca gacuuauacu aucuuacaau gaauaacaaa     1200

cauuggcuag uacauaaaga augguuccac gacauuccac uaccauggca cgcaggagca     1260

gacacaggaa caccacauug gaacaacaaa gaagcacuug uagaauuuaa agaugcccau     1320

gccaaaagac aaacaguagu aguauuagga ucacaagaag gcgcuguaca uacugcccua     1380

gccggcgcac uagaagccga aauggauggc gcaaaaggaa gacuaucaag ugggcauuua     1440

aaauguagac uuaaaaugga uaaacugcga cuaaaaggag uaucguacuc auuauguacg     1500

gcagcuuuua cauuuaccaa aauaccagca gaaacacuac acggaacugu aacgguggaa     1560

guucaauacg cuggcacaga uggaccuugu aaaguaccag cacaaauggc aguagacaug     1620

caaacuuuaa caccaguagg aaggcuaaua acagccaacc cgguaauaac ugaaaguaca     1680

gaaaauucaa aaaugaugcu agaauuagau ccuccguucg gagauagcua cauaguaauu     1740

gguguaggag aaaagaaaau aacccaucau uggcaccgaa guggaucuac aaucggaaaa     1800

gcauucgaag caacaguaag aggagccaaa agaauggcug uacuugggga uacagcaugg     1860

gauuuuggau caguaggggg agcgcucaau aguuuaggaa aaggaauaca ucaaauauuu     1920

ggagcagcuu uuaaaaguuu auuuggagga augaguuggu ucucacaaau auuaauaggu     1980

acccuacuaa ugugguuagg acuuaauacu aaaaauggaa guauaucauu gauguguuua     2040

gcauuaggug gaguucuaau auuuuuauca acagcaguaa gugcagaugu agguugcuca     2100

guagacuuca gcaaa                                                      2115


<210>  2496
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2496
aaaaaaagga gaggagcgga uacaucagua ggaauuguag gauuacuguu aacuacugca       60

auggcagcag aaguuacaag aagaggaagu gccuauuaua uguauuugga cagaaacgac      120

gcaggagaag caauaucauu uccaacaaca uuaggaauga auaaauguua uauacaaaua      180

auggauuuag gacauaugug ugaugccaca augucuuaug aauguccuau guuagacgaa      240

ggaguagaac cugaugacgu ugauuguugg ugcaacacaa caaguacuug gguaguauau      300

ggaacaugcc aucacaaaaa aggggaagca aggagaucaa gaagggcagu uacauuacca      360

ucucacucua cgagaaaauu acagacaaga ucacaaacgu gguuagaaag uagagaauau      420

acaaaacacu ugauacgagu cgaaaauugg aucuuccgga auccagguuu ugcgcuagca      480

gcagcagcaa uagcauggcu auuaggaagu ucaacaaguc aaaaaguaau auacuuagug      540

augauuuugu uaauugcacc ugcguacagu auaagaugua uaggaguguc uaauagagac      600

uuuguagaag gaaugucagg cggaacaugg guagauauug uacuagaaca cggaggaugc      660

gugacaguaa uggcacaaga caaacccaca guagauauag aauuaguaac cacaacagua      720

agcaauaugg cagaaguaag aagcuauugu uaugaggcaa guauaucaga uauggcaagu      780

gauucaagau guccaacuca gggagaagcu uauuuagaua aacaaagcga cacacaauau      840

guauguaaaa gaacacuugu agacagagga uggggaaacg ggugcgguuu auuuggaaaa      900

gguucauuag uaaccugugc aaaauuugca ugcucuaaaa aaaugacugg aaaaaguaua      960

caacccgaaa auuuagaaua uagaauaaug cuaaguguac acggaucaca acauucaggg     1020

augauaguaa augauacggg acaugaaaca gaugaaaaua gagcaaaagu agaaauaacu     1080

cccaacaguc caagagcaga agcgacacua gguggguuug guaguuuagg gcuggacugc     1140

gaaccaagaa cggguuuaga cuuuucagau uuguauuauc uaacuaugaa caauaaacau     1200

ugguuaguac auaaagagug guuucacgac auuccgcuuc cauggcaugc cggugcagac     1260

acaggaacac cacauuggaa caauaaagaa gcauuaguag aauuuaaaga ugcacaugca     1320

aaaagacaga ccguaguagu cuuaggaagu caagaaggag cgguacacac ugcacuagca     1380

ggagcacuag aagcagaaau ggacggagca aaaggaagac ugucaagugg acaccuaaaa     1440

uguagguuaa aaauggauaa acuaagauua aaagguguuu cauauucacu auguacagca     1500

gcauuuacau uuacgaaaau accagcagaa acauuacacg guacaguaac aguagaagua     1560

caguaugcag gaacagaugg accuugcaaa guaccugcac agauggcugu agauaugcaa     1620

acacuuacac caguaggcag acuaauaaca gcaaauccag uaauuaccga aaguaccgaa     1680

aauucaaaaa ugauguuaga acuugaucca ccuuuuggag acagcuauau aguaauaggg     1740

gugggagaaa aaaaaauaac acaccauugg cauagaagug gaaguaccau agggaaagca     1800

uuugaagcaa caguuagggg agcaaaaaga auggcgguau uaggggacac agcuugggac     1860

uuuggaagug uugguggagc auuaaauuca uuaggaaaag gaauacauca aauauuugga     1920

gcagcauuua aaucauuauu uggagggaug aguugguuua gucaaauauu aauaggaaca     1980

cuauuaaugu gguuaggguu aaauacuaaa aauggcagua uaagcuuaau guguuuagca     2040

cuagggggag ugcuaauauu ucuaaguaca gccguaagug cagacgucgg augcaguguu     2100

gauuuuucca aa                                                         2112


<210>  2497
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2497
aaaagaagag gugcagacac gaguguugga auagugggau uguuacuaac aacagcaaug       60

gcugcagagg uuaccagaag aggaucugca uauuauaugu auuuagacag aaacgaugcc      120

ggugaagcaa uaaguuuccc aacgacucua ggaaugaaua aaugcuacau acaaauuaug      180

gacuuaggac auaugugcga ugcaacaaug agcuaugaau gcccaauguu agaugaggga      240

guagaaccag acgauguaga uuguuggugu aauacaacau caacuugggu aguuuacggu      300

acuugccauc auaaaaaagg agaagcaaga agaucaagaa gagcaguaac auugccuagu      360

cauucaacac gcaaacucca aacaagauca caaacauggu uggaaucaag agaauacaca      420

aaacauuuaa uaagaguaga aaauuggauc uuucgaaauc caggauuugc acuagcagca      480

gcagcaauag caugguuauu aggaaguucu acaucacaaa aaguaauaua ccuagucaug      540

auacucuuga uagccccugc auauucuaua agaugcauag gaguuaguaa uagagauuuc      600

guugaaggaa uguccggagg aacuuggguu gauauagucu uagaacacgg aggaugugua      660

acuguaaugg cacaagauaa accaacugua gauauagaau uaguuacaac aacaguaucu      720

aauauggcag agguacgauc auacuguuau gaggccagua uaagugauau ggcauccgac      780

ucacgauguc caacacaagg agaagcuuac cuugauaaac aaagugauac gcaauaugua      840

uguaaaagaa cauuaguaga ucgaggaugg ggcaauggcu gcggauuauu uggcaaagga      900

uccuuaguaa caugugcaaa auucgcaugu agcaaaaaaa ugacaggaaa aucaauacaa      960

ccagaaaauu uagaauauag aauaaugcua ucaguacaug gaucacaaca uagcggaaug     1020

auaguuaacg auaccggaca ugaaaccgac gaaaauagag caaaaguaga aauaacacca     1080

aacagcccaa gggcagaagc aacauuaggc ggauuuggau ccuuagguuu agacugugaa     1140

ccacgcacug guuuggauuu uagugauuua uacuaccuua caaugaacaa uaaacauugg     1200

uuaguccaca aagaaugguu ucaugauaua ccauuaccau ggcaugcugg ugcagauacu     1260

gggaccccac auuggaauaa uaaagaagca uuaguagagu uuaaagaugc acaugcaaaa     1320

agacaaacag ucgucguccu agggagucaa gaaggagcag uacacacagc acuagcagga     1380

gcccuugaag cagaaaugga cggagcaaaa ggaagauuaa guucaggaca uuuaaagugu     1440

cgccuuaaaa uggacaaauu aagacuaaaa ggagucaguu auucgcuaug uaccgcagcg     1500

uucacguuua caaaaauucc ggcagaaacu cuacauggua cuguuacugu agagguccaa     1560

uaugcaggca cagacggacc auguaaagua ccagcucaaa uggccgucga caugcaaaca     1620

cucacuccag uaggaagauu aauaacagca aauccaguaa uuacagaauc aacagaaaau     1680

aguaaaauga uguuagaauu ggauccaccc uuuggagaca guuauauugu aauaggaguc     1740

ggagaaaaaa aaauaacaca ucauuggcau cgaucaggau caacgauagg aaaagcauuu     1800

gaggcaaccg ucagaggagc uaaaagaaug gcaguucuag gagacaccgc augggauuuc     1860

ggaucagucg gaggggcacu aaauucucuc gguaaaggga uacaucaaau auuuggagcc     1920

gcauuuaaau ccuuauucgg agguaugagu ugguuuucac aaauccuaau aggaacauug     1980

cuaauguggc ugggacuaaa uacgaaaaau gguaguauau cauugaugug cuuagcucua     2040

ggagguguau uaaucuuucu uagcacagca guuucggcag acgucggaug uaguguagau     2100

uucaguaaa                                                             2109


<210>  2498
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2498
agaagaggag ccgacacuuc agugggaauu guaggauuau uauuaacaac cgcaauggca       60

gcagaaguua caagaagggg aucagcauau uauauguauc uugauagaaa ugacgcaggu      120

gaagccauua guuuccccac aaccuuaggu augaauaaau gcuauauaca aauaauggau      180

uuaggacaua ugugugaugc aaccaugagu uacgaaugcc caauguuaga cgaagggguu      240

gaacccgaug auguagauug cugguguaac acuacaagua cauggguagu auauggcaca      300

ugucaucaca aaaaagguga agcaagaaga ucaagacgag caguuaccuu accuagccau      360

aguacacgca aauuacaaac aagaucacag acuugguuag aaaguaggga auauaccaaa      420

cacuuaauaa gaguugaaaa uuggauauuu agaaaucccg gauuugcauu agcagcagca      480

gcaauagcgu gguuacuagg cagcucaacu agucaaaaag uaauauaucu aguaaugauc      540

uuauuaauag cuccagcaua uucaauaaga uguauaggag ugagcaauag agauuuugua      600

gaaggaauga guggagguac auggguagac auaguacuag aacacggagg cuguguaaca      660

gucauggcac aagauaaacc aacaguugau aucgaauugg uaacaacaac aguaagcaac      720

auggcggaag uaagaagcua cugcuaugaa gcuaguauaa gcgacauggc aagugauuca      780

agauguccua cacaaggaga agcguaucua gauaaacaaa gugauacaca auauguuugu      840

aaaagaacau uaguagauag aggaugggga aauggaugug gauuguuugg uaaaggguca      900

uuggucacuu gcgcuaaauu ugcauguagu aaaaaaauga caggaaaaag cauucaacca      960

gaaaaccuag aauauagaau aaugcuuagu guccauggau cacagcauuc agguaugaua     1020

guaaaugaca caggacauga aacagacgaa aauagagcaa aaguagaaau aacaccgaac     1080

agcccaagag cagaagcuac uuuaggagga uuuggaaguu uaggacuaga cugugaacca     1140

agaacaggcu uagauuuuag ugacuuauau uauuugacga ugaauaauaa acacugguua     1200

guacacaaag aaugguucca ugauauacca uugccauggc augcaggugc agauacagga     1260

acaccacacu ggaauaacaa agaggcccug guagaguuca aagaugcaca ugcaaaaagg     1320

caaacaguug uaguauuagg aucucaagaa ggugcaguuc acacugcccu agccggagca     1380

uuagaagccg aaauggaugg agcuaaagga agauugagua gcggacaucu uaaaugcaga     1440

uuaaaaaugg auaaauuaag acucaaaggu guaagcuaua guuuauguac agcagccuuu     1500

acauuuacua aaauacccgc agaaacacua cauggaacag uuacaguuga aguacaauac     1560

gcaggaacgg augguccaug uaaaguaccu gcucaaaugg caguagacau gcagacacua     1620

acgccgguag guagauuaau aacagcgaau cccguuauaa cugaaucaac agaaaauuca     1680

aaaaugaugc uagaauuaga uccaccuuuu ggagacagcu auauaguaau aggaguagga     1740

gaaaaaaaaa uaacacauca cuggcauaga ucaggcagca caauugguaa agcuuuugaa     1800

gcuacaguuc gaggagcaaa aagaauggca guacuaggag auacagccug ggauuuugga     1860

agcguaggug gagcuuuaaa cucuuuggga aaagguauac aucaaauuuu uggagcugcu     1920

uuuaagaguu uguuuggugg aaugucgugg uuuagucaaa uauugauagg cacauuacuc     1980

augugguuag gacuaaauac aaaaaacgga ucaauaaguc uaauguguuu agcacuagga     2040

ggaguauuaa uauuuuuauc uacagcagua agcgcagacg uuggauguuc aguagauuuc     2100

aguaaa                                                                2106


<210>  2499
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2499
aggggagcag auacaucagu aggaauagua ggauuauuau uaacaacagc aauggcagca       60

gaaguuacaa ggaggggaag ugccuacuac auguacuuag auagaaacga ugcaggagaa      120

gcaauuaguu uuccuacaac auuaggaaug aauaaaugcu auauacaaau aauggacuua      180

ggucauaugu gcgacgcaac uaugucuuac gaaugcccaa uguuagauga aggaguagaa      240

ccagacgaug uagacuguug guguaauacc acaaguacgu ggguaguaua uggaacuugu      300

caucacaaga aaggagaagc aagaagaucc aggagagcag uaacuuuacc aucacacuca      360

acgagaaaau uacagacaag aagccaaaca ugguuagaau caagagaaua uacaaaacac      420

uuaaucagag uagaaaauug gauauuucga aauccaggau ucgcacuugc ggcagcugcu      480

auagcauggc ucuuaggaag uucuaccagc caaaaaguaa uuuauuuagu aaugauacuu      540

uuaauagcac ccgcauauuc aauaaggugu aucggaguuu cuaauagaga uuuuguggaa      600

ggaaugucag gcggaacuug gguggacauc guuuuagaac acggaggaug uguuacagua      660

auggcucaag auaaaccaac uguagauaua gaauuaguaa cuacgacagu gucaaauaug      720

gcagaaguaa gaucuuauug uuaugaggcu ucaaucagug acauggcauc agauaguaga      780

uguccaacac aaggagaagc auauuuagac aaacaaagug acacacaaua ugucuguaaa      840

aggacauuag uagacagagg guggggaaau ggauguggac ucuuuggaaa aggaucucua      900

guaacuugcg cuaaauuugc auguucaaag aaaaugacag gaaaaucaau acaaccagaa      960

aaucucgaau auagaauuau guuaucagua cauggcucac aacauagugg aaugauaguu     1020

aaugauacag gacaugaaac agaugaaaac agagcaaaag uagaaauuac accuaacuca     1080

ccuagagccg aagccacacu aggugguuuu ggaaguuuag gacuagauug ugaaccaagg     1140

acaggucuag auuuuagcga cuuauauuau uuaacaauga auaauaaaca uugguugguc     1200

cauaaagaau gguuccacga uauuccauua ccuuggcaug caggagcaga uacaggaaca     1260

ccacauugga auaacaaaga agcauuaguu gaauuuaaag augcacaugc uaaaagacaa     1320

acuguaguug uacuuggauc ccaagaagga gcaguucaua cagcauuagc gggugcacuu     1380

gaagcagaaa uggauggagc aaaagguaga cuaucaagcg gacaucugaa augucgacua     1440

aaaauggaua aacugagauu gaaaggaguu aguuauaguu uaugcacagc agcauucaca     1500

uuuacaaaaa ucccagcaga aacauuacau ggaacaguaa cuguagaagu acaauaugcc     1560

ggaacugaug gacccuguaa aguucccgca cagauggcgg uagacaugca gacauuaacu     1620

ccaguaggua gauuaauaac ggcaaauccu gucauaacgg aaucuacaga aaauucaaaa     1680

augauguugg aauuagaccc accauuugga gauuccuaca uaguuauagg agucggugaa     1740

aagaaaauaa cacaucauug gcacaggagc ggaaguacca uugguaaggc uuuugaagca     1800

acaguaagag gagcaaaaag aauggcaguu uugggagaua cagcauggga uuucgguagu     1860

guuggaggag cucuuaauuc uuuaggcaaa ggaauacauc aaauauucgg agcagcuuuu     1920

aaaagucuau ucggagggau gaguugguuu agccaaauac uaauuggaac gcuacugaug     1980

ugguuaggac ucaacacuaa aaacgggagc auuaguuuaa ugugccuagc auuaggugga     2040

guacuaauau uucuaucaac agcaguuuca gccgaugucg gcuguucagu agauuuuucc     2100

aaa                                                                   2103


<210>  2500
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2500
ggugcagaua caucaguugg aauaguagga cugcuauuaa caacagcaau ggcggcagaa       60

guaacaagac ggggaucagc uuauuacaug uaucuagaua gaaaugaugc aggagaagca      120

auaucauucc caacgacacu aggaaugaac aaauguuaca uccaaauaau ggaccuagga      180

cauaugugcg acgccacuau gagcuaugaa ugcccaaugc uagaugaagg aguagaaccg      240

gaugauguug acuguuggug uaauacgaca ucaacuuggg uaguauacgg aacuugucau      300

cauaagaaag gagaagcaag acgaaguaga cgagcaguaa cacuaccauc acacaguaca      360

agaaaauuac aaacaagaag ucagacaugg cuugaaagca gagaauauac aaaacauuua      420

auaagaguag aaaauuggau uuuuagaaau ccagguuucg ccuuagcugc agcugcgaua      480

gcauggcucu ugggaucuag cacuucacaa aaaguaauau acuuaguaau gauuuuacua      540

auugcaccag cauauucaau cagauguauu ggagucagca auagggacuu uguagaaggc      600

augucaggug gaacgugggu agauaucgua cuagaacacg gagggugcgu aacaguaaug      660

gcacaggaua aaccaacggu agacauagaa uuaguaacga caacgguauc aaauauggca      720

gaaguaagaa guuacuguua cgaggcaucu auuagugaca uggcuucaga cucacgaugu      780

ccaacacaag gagaagcaua uuuagauaaa caaagugaua cgcaauacgu augcaaaaga      840

acauuaguag acagagggug ggguaaugga ugcgguuuau uuggaaaagg aucauuagua      900

acaugugcaa aauuugcaug uucuaagaaa augacaggaa aaaguaucca accagagaau      960

uuagaauaca ggauuauguu aaguguacac ggaucacaac auucagggau gauaguaaau     1020

gacacaggac augaaacuga ugaaaaucga gcaaaaguag aaauaacucc caauucacca     1080

agagcugaag cuacauuagg gggauuugga ucacuaggac uagauuguga accgagaaca     1140

ggauuagauu uuagugauuu auauuauuua acaaugaaca auaaacacug guuaguacau     1200

aaagaauggu uucaugacau accauuaccg uggcacgcag gggcagauac uggaacacca     1260

cauuggaaua auaaagaggc acugguugag uucaaagacg cucacgcaaa aagacaaaca     1320

guagucgugu uaggcucuca ggaaggagca guacauaccg cguuagcagg cgcacuagaa     1380

gcagaaaugg auggcgcuaa aggcagacuu agcagcggac acuugaaaug cagacuaaaa     1440

auggacaaau uacgguuaaa aggcgugagu uauucauuau guacagccgc auucacauuc     1500

acaaaaauac cugcugaaac uuuacauggg acagugacgg uagaaguaca guaugcgggg     1560

acagacggac cuuguaaagu accugcacaa auggcaguag auaugcaaac uuuaacacca     1620

guaggaagau uaauaacagc aaauccagua auuacagaaa guacagaaaa uaguaaaaug     1680

augcuagaau uagacccacc uuuuggcgac ucauauauag ucauaggagu aggugaaaag     1740

aaaauaacac accauuggca caggagugga ucuacaauag gaaaagcauu ugaagccaca     1800

guaagaggag ccaaaagaau ggcaguacua ggagacacag cuugggauuu uggaagcguc     1860

ggaggagcac uaaauaguuu aggaaaagga auacaucaaa uauucggagc agccuucaaa     1920

ucauuauuug gugguauguc cugguuuagu caaauauuaa uaggaacauu auuaaugugg     1980

uuaggacuaa auacuaaaaa cgguaguauc ucacuuaugu guuuagcccu ggguggagua     2040

uuaauauuuu uaucaaccgc uguaucagcu gauguuggau guucaguaga uuucucaaaa     2100


<210>  2501
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2501
gcagauacau ccguaggaau aguaggacuu uuauuaacaa cagcaauggc agcggaagua       60

acaagaagag guucagccua uuacauguau uuggauagga augacgcagg agaagcaauu      120

agcuucccua caacacuugg gaugaacaaa uguuauauac aaaucaugga ccuaggacau      180

augugugaug caacuaugag uuacgaaugu ccaaugcuug augagggagu agaaccagac      240

gacgucgauu guuggugcaa uacuacaagu acuuggguag uauaugguac augucaucau      300

aaaaaaggag aagcaagacg cucaaggaga gcaguaacau uaccuaguca cucaacaaga      360

aaacugcaaa cacgaaguca aacauggcuc gagagccgag aauauacaaa acaucuaauu      420

agaguagaga acuggauauu cagaaaucca ggauucgccu uagcggcagc agcaauagca      480

ugguuacuag gaaguucaac aucacaaaaa guaauauauc uagucaugau uuuauuaaua      540

gcuccugcau auucuauacg auguauagga gucaguaaua gggauuuugu agaaggaaug      600

aguggaggua ccuggguaga uauuguauua gaacacggag gcuguguaac uguuauggca      660

caagauaaac cuacaguaga uauagaacua guaacgacaa cagugagcaa cauggcugaa      720

gugagaucau auuguuauga agcuagcaua agcgauaugg ccagugauuc uagaugucca      780

acccaaggag aagcauacuu agauaagcaa ucugauaccc aauauguaug caaaagaaca      840

cuaguagacc gaggaugggg caauggaugc ggucuauucg gaaaaggaag cuuagugaca      900

ugugcaaaau uugcauguuc caaaaaaaug acaggcaaaa gcauacaacc agaaaauuua      960

gaauacagga uaaugcuauc aguacaugga ucucaacaua gcggaaugau aguaaaugau     1020

acaggacacg aaacugacga aaauagagcc aaaguagaga ucacacccaa caguccaaga     1080

gcagaagcaa cgcuaggagg auuuggaagc cucggauuag auugugaacc aagaacagga     1140

cuagauuuca gcgaucuuua cuauuuaacc augaacaaca aacauugguu aguacauaaa     1200

gagugguuuc augauauacc guuaccuugg caugcaggag cagauacagg aacaccacac     1260

uggaauaaua aagaagcccu uguagaauuu aaagacgcac augcaaaaag acaaacggua     1320

guuguauuag guagucaaga aggagcagua cauacagcac uagcuggugc auuagaagca     1380

gaaauggacg gagcaaaagg aagauuaagc agcggacauc uaaagugcag acuaaaaaug     1440

gauaaacuuc gccuaaaagg aguaucauac ucauuaugua cugccgcuuu uacauucacc     1500

aaaauaccug cugaaacuuu acacggaaca gucacuguag aaguacaaua ugcagguaca     1560

gaugguccau guaaagugcc cgcucaaaug gcaguagaua ugcaaacacu aacaccugua     1620

ggaagauuaa uaacagcaaa cccuguaaua acagaaucaa cagaaaacuc gaaaaugaug     1680

cucgaacuag acccaccguu uggagauagu uacauuguaa uugguguagg agaaaaaaaa     1740

auuacacauc auuggcaccg uucagggagu acuauaggaa aagcauuuga agcaacugua     1800

agaggagcaa aaagaauggc aguauuaggu gauacagccu gggacuucgg uaguguagga     1860

ggagcauuga acucccuagg aaaaggcauu caucaaauau uuggagcagc auuuaaaagu     1920

uuauuuggag gaaugucaug guuuagucaa auauuaauag gcacgcuacu uaugugguua     1980

ggacuaaaua caaaaaacgg aaguauuagc cuaaugugcu uagcuuuagg gggcguguua     2040

auuuuuuuga gcacagcagu uagcgcagac guaggaugua guguugauuu uucaaaa        2097


<210>  2502
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2502
gacacuagug uaggaauagu aggucuacua cuaacaacag caauggcagc agaaguuacu       60

cgaagaggua gcgcauauua uauguauuua gauagaaaug acgcaggaga agcuauaagu      120

uucccaacaa cacuaggaau gaacaaaugu uacauucaaa uaauggaccu gggacauaug      180

ugcgaugcaa ccaugucuua cgaauguccu augcuugacg aaggaguaga accugaugau      240

guugacuguu gguguaauac aaccagcacu uggguaguau auggaacaug ucaucauaaa      300

aaaggggaag caagacgauc aagaagggca gugacauugc caagucacuc aacuagaaaa      360

uuacaaacua gaucacaaac augguuggaa ucaagagaau acacaaaaca uuuaauuaga      420

guggaaaauu ggauauuccg aaacccagga uucgcauuag cggcagcagc aauagcuugg      480

uuauugggaa gcucaacgag ucaaaagguu auauaccuag ugaugauacu auuaauagcu      540

ccagcauauu ccauaagaug cauaggagua aguaaccgag acuuuguaga aggaaugagu      600

ggaggaacau ggguagauau aguauuggaa cauggaggau guguaacagu aauggcacaa      660

gauaaaccaa caguagauau agaacuaguu acaacaacag uuucaaauau ggccgaagua      720

agaucguacu guuaugaggc uagcauauca gacauggcau cugauucaag auguccaacu      780

caaggcgaag cauacuuaga uaaacagagu gacacgcaau auguaugcaa aagaacacuc      840

guggauagag gaugggguaa cggguguggc cuguucggca aagguagucu aguaacaugu      900

gcuaaauuug cuuguucaaa aaaaaugaca ggaaaaucaa uacaaccgga aaaccuagaa      960

uacagaauaa uguuaagugu ccacgggagu caacauucag gaaugauugu uaaugauaca     1020

ggucaugaga cugaugaaaa uagagcaaaa guagagauca caccaaauuc accacgagca     1080

gaagcaacac uaggugguuu uggaaguuua ggauuagauu gugaaccaag aacaggauua     1140

gauuuuucag acuuguauua cuuaacaaug aauaauaaac acugguuagu ccacaaagaa     1200

ugguuucaug auauuccacu gccauggcau gcaggagcag auacaggcac accacacugg     1260

aauaacaaag aagcauuagu ugaauuuaaa gaugcacacg cuaaaagaca aacaguaguc     1320

guauuaggaa gucaagaagg agccguucac acagcacuag caggugcauu agaagcugaa     1380

auggacggag cuaaagguag auuaucaagu ggacaccuaa aaugcagacu gaaaauggac     1440

aaauuaagac uaaaaggagu aaguuauagc uuauguacag cggcauuuac auuuacaaaa     1500

auaccggcag aaacauuaca ugguacagua acuguagaag uacaauacgc cggaacagau     1560

ggaccaugca aaguaccugc ucaaauggcc gucgauaugc aaacccuaac accgguugga     1620

agacucauaa cagcaaaucc aguaauaaca gagucaacag aaaauaguaa aaugauguua     1680

gaauuagacc caccauuugg ugacucguac auagugauag gaguugguga aaaaaaaaua     1740

acacaccauu ggcacagaag uggaagcacu auuggaaaag cauuugaagc aacuguuaga     1800

ggcgcaaaaa gaauggcagu ucuuggagau acagcauggg auuuuggaag uguuggagga     1860

gcauuaaaca guuuaggaaa aggcauacau caaauuuuug gagcagcuuu uaaaucacua     1920

uuuggaggaa ugucaugguu uucacaaaua uuaauuggua cuuuacuaau gugguuagga     1980

cuaaauacua aaaaugguag uauaaguuua augugucuag cauuaggcgg agucuuaauc     2040

uucuuaucaa cagcaguaag cgcagacgua ggguguagug uagacuucuc aaaa           2094


<210>  2503
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2503
accagcguug gaauaguagg uuuacuauua acaaccgcaa uggcagcaga agugacaaga       60

agaggaagug cauacuauau guacuuagau cguaacgaug caggugaagc uauaucuuuu      120

ccaacaacgu uaggaaugaa uaaauguuac auacaaauaa uggacuuagg acauaugugu      180

gacgcaacua ugucauauga auguccaaug uuagaugaag gaguugaacc agacgacgua      240

gauuguuggu guaauacuac caguacaugg guaguauaug gaacaugcca ucauaaaaaa      300

ggagaggcua gaagaaguag gagagcagua acauuaccau cucauucaac aagaaaacuu      360

caaacacgau cacaaaccug gcuagaaagc cgagaauaca caaaacaucu aauacgagug      420

gaaaauugga uauuuagaaa uccaggauuc gcacuagcug cagccgcuau cgcauggcua      480

cuagguaguu caacaucaca aaaaguaauu uacuuaguaa ugauccuacu cauugccccg      540

gcauauucaa uaagaugcau cgggguuagc aacagagacu uuguugaagg caugucggga      600

ggcacauggg uagauauugu acuugagcac ggagggugcg uaacuguuau ggcucaagau      660

aaaccaacag uugacauaga acuaguaaca acaacaguau caaacauggc ggaaguaaga      720

aguuauugcu augaagcaag cauaucugac auggcaucag auucuagaug uccaacacaa      780

ggagaagcuu auuuagauaa acaaucagac acacaauacg uauguaaaag aacauuagua      840

gacagaggcu ggggaaaugg auguggauug uuuggaaaag gaucauuggu aacaugugcc      900

aaauucgcau guaguaaaaa aaugacugga aaaaguaucc aaccagaaaa uuuagaauau      960

cgaauuaugu uaagcgucca ugguucccaa cauucaggua ugauaguaaa cgauacagga     1020

cacgagacug augaaaauag agcaaaagug gaaauaacuc caaauagucc aagagcagaa     1080

gcaacuuuag gugguuuugg aucccucgga uuggauugug aaccaagaac cggauuagac     1140

uucagugauc uauacuacuu aacaaugaau aauaaacacu ggcuugucca caaagaaugg     1200

uuccacgaca ucccauuacc auggcacgca ggcgcagaua caggcacccc acauuggaau     1260

aauaaagaag cacuaguaga auucaaagac gcacaugcaa aaagacaaac gguaguagua     1320

uuaggaaguc aagaaggagc uguacauaca gcccuagcug gagcacuaga agcagaaaug     1380

gacggagcaa aagguagauu aaguagugga caccugaaau gcagauuaaa aauggauaaa     1440

cuaagguuaa aaggaguuag cuauucuuua uguacugcag cauuuacauu cacuaaaaua     1500

ccagcagaaa ccuuacaugg aacagugaca guagaaguac aauaugcagg aacugaugga     1560

ccauguaaag uaccagcaca aauggcaguu gauaugcaaa cauuaacucc aguagggaga     1620

cuuauaacag caaauccagu aauaaccgaa ucaacagaaa auagcaaaau gauguuagaa     1680

uuagacccac cuuucggcga cagcuacaua gucauaggag uuggugaaaa aaaaauaacc     1740

caucauuggc auagaagugg aagcacaaua ggcaaagcau uugaagcaac ugucagaggg     1800

gccaaaagaa uggcaguucu aggagauacc gcaugggacu ucggaagugu aggaggagca     1860

cuaaacaguu uaggaaaagg aauacaucag auauuugggg cagcauucaa aagucuauuu     1920

gggggcauga gcugguuuuc acaaauuuua auuggaacau uguuaaugug gcuaggauua     1980

aauacaaaaa augguagcau aagcuuaaug ugucuagcuc uaggaggagu acugauuuuu     2040

uuaagcacag cuguuagugc ugauguaggg uguucuguag auuuuucaaa a              2091


<210>  2504
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2504
acaaguguug guauaguggg guuauuauua acgacugcaa uggcagcagc aguaacaaga       60

agaggaucag cuuauuauau guauuuagac agaaaugaug caggggaagc aaucucauuu      120

cccaccacuc uagguaugaa uaaauguuau auucaaauaa uggaucucgg ucauaugugu      180

gaugcuacua ugaguuauga auguccaaug uuagaugaag gaguugaacc agaugauguc      240

gacuguuggu gcaauacaac aaguacuugg guaguauaug gaaccuguca ucauaagaaa      300

ggagaagcua gacguaguag gagagcagua acacuuccaa gucauucaac aagaaaacua      360

caaacuagaa gccaaacaug guuagaaagu agagaauaua caaaacaccu aauaagagua      420

gaaaacugga uuuuuagaaa cccaggauuu gcccuggcag cugcugcuau agccugguua      480

cugggaucca guacaucaca aaaaguaaua uacuuaguaa ugauauuauu gauagcuccc      540

gcuuauagca uaagauguau uggcgucucu aauagagauu ucguagaagg gaugaguggu      600

gggacauggg uagauauagu auuagaacac ggagguugcg uaacaguaau ggcacaagac      660

aaaccaacag uagauauaga auuagugaca acaacaguga gcaauauggc ugaaguacga      720

ucauauugcu augaagcauc aaucucagau auggcuucag acucaagaug uccaacgcaa      780

ggagaagcau aucuagacaa acaaagugau acacaauacg uauguaaaag aacauuagua      840

gauagagguu gggguaaugg uuguggacua uuuggaaaag gcuccuuagu uacaugugcu      900

aaauucgcuu gcuccaagaa aaugacagga aaaucaauac aaccugaaaa ucuugaauac      960

agaauaaugc uaaguguaca uggaagccaa cauaguggga ugauaguaaa ugauacggga     1020

caugaaacug augaaaauag agcaaaagua gaaauaacuc caaauagccc aagagcagaa     1080

gcaacuuuag gcggauucgg cagccuugga uuagauugug aaccaagaac aggcuuagau     1140

uuuagcgacu uauauuacuu aacaaugaau aacaaacauu gguuagucca uaaagaaugg     1200

uuucacgaua uaccauuacc auggcaugcg ggugcugaua ccgguacacc acauuggaau     1260

aacaaagaag cauugguaga guucaaagac gcacacgcaa aaaggcaaac agucguaguc     1320

uugggaagcc aagaaggagc cguacauacc gcauuagcag gagcacuaga agcagaaaug     1380

gacggagcaa aaggaagauu aagcagugga cauuuaaaau gucgauuaaa aauggauaaa     1440

cuuagauuaa aaggagucuc auauucacuu uguacagcag cauuuacuuu uacaaaaaua     1500

ccugcagaaa cauuacaugg gacagugaca guggaaguac aauaugcagg aacagaugga     1560

ccauguaaag uaccagcuca aauggcuguu gauaugcaaa cacuaacacc aguaggacga     1620

cuaauaacug cuaauccagu aauaacagaa ucaacugaaa auagcaaaau gaugcuagaa     1680

cuugacccgc cuuuugguga caguuauaua guuauaggug ucggugaaaa gaaaaucaca     1740

caucacuggc auaggagugg gucaacaaua gggaaagcgu uugaagcaac agucaggggu     1800

gcaaaaagaa uggcuguacu aggagauaca gcaugggauu uuggaagugu aggaggggca     1860

uugaauaguc uaggaaaagg uauacaucaa auauuugggg cagcauuuaa aagucuauuu     1920

ggcggaaugu caugguuuuc acaaauucua auaggaacuu uacuuaugug gcucggauug     1980

aauacgaaaa auggaucaau cagcuuaaug uguuuagcuu uaggaggugu gcuaauauuu     2040

uuaaguacag ccguaucagc cgauguagga uguucuguag auuuuaguaa a              2091


<210>  2505
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2505
agaggagcag auacaagugu ugggauagua ggacugcuuc uaacuacugc aauggccgcu       60

gcgguaacga gacgaggaag ugcauauuac auguauuuag acagaaacga ugcaggagaa      120

gcuauaaguu uuccaacaac gcuagguaug aacaaaugcu acauacagau aauggaucua      180

ggacacaugu gugacgcaac uaugucauac gaaugcccaa uguuagauga agguguagaa      240

ccagaugaug ucgacuguug gugcaacaca acuucgacau ggguaguaua uggaacaugu      300

caucauaaaa aaggagaagc acgccgauca agaagggcag ucacauuacc aucacacagu      360

acaaggaaau uacaaacaag aagucaaaca uggcuugaau caagagaaua uacaaaacac      420

uuaauuaggg uagaaaauug gauuuucaga aauccaggau uugcauuagc agccgcagca      480

auagcuuggu uauuaggaag uaguacaagc caaaaaguaa uuuauuuagu uaugauacuu      540

uuaauagcuc cagcauauuc aauacgaugu auagguguau caaacagaga uuuuguugaa      600

ggaaugagug gaggaacaug gguagauaua guucuagaac auggagguug uguaacagua      660

auggcacagg acaaaccaac aguagacaua gaauuaguaa caacaacagu uucaaauaug      720

gcagaaguaa gaucuuauug uuacgaagca aguauaagcg auauggcuag cgauucacga      780

ugucccacac aaggagaagc uuaucuagau aaacaaucag auacacaaua cguauguaaa      840

cgaacauugg uagacagagg auggggaaau ggauguggau uauuuggaaa agguagccua      900

guaacaugcg caaaauucgc cuguaguaaa aaaaugacag gaaaaucaau ucaaccagaa      960

aacuuagaau auagaauaau gcuauccguu cacggcucgc aacauucagg gaugauugua     1020

aaugauacag gacaugaaac agaugaaaac agggcuaagg uagaaauaac ucccaauuca     1080

ccaagagcug aagcaacauu aggcggguuu ggaucacucg gucuagacug ugaaccacga     1140

acuggauuag auuuuucaga ucucuacuau cugacuauga auaauaaaca uugguuaguc     1200

cauaaagaau gguuucacga uauaccacua cccuggcaug caggugcaga uacuggaaca     1260

ccucacugga auaauaaaga agcucuugua gaguuuaaag augcacaugc aaaaagacaa     1320

acaguaguag uauuaggaag ucaagaagga gcaguacaca cagcauuagc cggugcucua     1380

gaagcagaaa uggacggagc aaaaggcaga cucucaagug gacauuuaaa augucgauua     1440

aaaauggaca aauuaagguu aaaaggugua aguuacaguc uauguacagc ugcauuuaca     1500

uuuacaaaaa uaccugcuga aacgcuccau ggaacaguca caguugaagu ccaauaugcc     1560

ggaaccgaug gcccauguaa agucccugca caaauggcgg uagacaugca aacauuaaca     1620

ccaguuggua gauuaauaac cgcaaauccg guuauaacag aaaguacaga aaauuccaaa     1680

augauguuag aauuagaucc accauuugga gacuccuaua uaguaauugg aguaggagaa     1740

aaaaaaauaa cacaucauug gcacagauca gguucaacaa uaggaaaagc guucgaagcg     1800

acagugagag gagcaaaaag aauggcugua uuaggagaua cagcuuggga cuuugggucc     1860

guaggcggug cacuuaauuc acuuggaaaa ggcauacauc aaauuuucgg ggcggcuuuu     1920

aaaucacuuu ucggaggaau gucaugguuu ucacagauau uaauaggaac auuauuaaug     1980

ugguuaggac uuaauacaaa aaauggaagc auuaguuuaa ugugccuagc gcugggaggu     2040

guacuuauau uucuuucaac cgcaguaagu gcugauguag gguguucugu agacuuuucc     2100

aaa                                                                   2103


<210>  2506
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2506
agaggacugu caucgagaaa aagaagaagc caugaugucc uaacaguuca auuucuaaua       60

uuagggaugu uacuaaugac gggaggagua acuagacgug gaucagcaua uuauauguac      120

cuagauagaa acgaugcugg ugaagcaaua ucauuuccaa caaccuuagg aaugaauaaa      180

uguuacauuc aaauuaugga uuugggucau augugcgaug caaccauguc cuaugaaugu      240

ccaauguuag acgaaggagu ggaaccagau gauguagauu guuggugcaa uacaacguca      300

acuuggguag uauauggaac uugucaucau aagaaaggag aagcaagaag aucuagacga      360

gcaguaacau uaccaucaca uucaacuaga aaacuacaaa caagaagcca aacaugguua      420

gagaguagag aauacaccaa acaucuaaua aggguugaaa auuggauauu ucguaauccu      480

gguuucgcau uagcagcagc agcuauagca ugguuauuag gaagcaguac aucacaaaaa      540

guaauauacu uagucaugau auuacuaauc gcaccagccu auucaauaag auguauagga      600

guuaguaaua gagauuuugu ggaagguaug agugguggaa cauggguuga caucguacua      660

gaacauggag gguguguuac uguaauggca caagauaaac cgacaguuga uaucgaauua      720

gugacaacaa caguaucuaa uauggcagaa guaagaagcu auuguuauga agcuagcaua      780

uccgauaugg caucagacag cagauguccc acacagggag aagcauaucu ugauaaacag      840

ucagacacac aguauguaug caaaagaaca uuaguagaca gagggugggg aaacggaugu      900

gguuuauuug gaaaaggauc auuaguaacc ugugcuaaau uugcaugcag caagaaaaug      960

acuggcaaaa guauacaacc ggaaaaucua gaauaccgaa uaaugcucag uguucacgga     1020

ucacaacauu cagguaugau uguaaacgau acuggacaug aaacagauga aaacagagca     1080

aaagucgaaa uaacuccaaa caguccacga gcagaagcua cuuuaggcgg uuuuggaucc     1140

cuuggauuag auugugagcc acgaacugga uuagauuuca gugauuuaua uuauuuaaca     1200

augaauaaua aacacugguu aguacacaaa gaaugguucc augauauacc auuaccuugg     1260

caugcaggag cugauacagg gacaccacac uggaauaaca aagaagcccu aguagaauuu     1320

aaagaugcac augcgaaacg acaaacaguu guugucuuag gaucucaaga aggugcagua     1380

cauacagcau uagcaggagc ccuagaagca gaaauggacg gugcuaaagg gcgauuaucu     1440

agcggacauu uaaaauguag auuaaaaaug gauaaauuaa gacuaaaagg uguuagcuau     1500

aguuuaugua cagccgcauu cacauucacu aaaauaccug cagagacucu acauggaaca     1560

guaacuguag aaguacaaua ugcagguacu gacggaccuu gcaaaguucc agcacaaaug     1620

gcaguugaca ugcaaacacu aacuccagua ggaagauuaa uaacggcgaa cccaguuaua     1680

acagaaucaa cugaaaauag caaaaugaug cuugaacuag auccaccauu uggagacuca     1740

uauauaguaa uaggaguagg cgaaaagaaa auaacacauc acuggcauag aagugguagc     1800

acuauuggua aagcauuuga agcaacugua agaggugcaa aaagaauggc aguauuggga     1860

gauacugcau gggauuuugg aucaguaggc ggggcacuaa auucacuugg gaaaggaaua     1920

caucaaauau uuggagcagc auuuaaaucu uuauuuggag gcaugucaug guucucacaa     1980

auauugauug gaacccuacu uauguggcua gguuuaaaca cuaaaaaugg aucaauuuca     2040

uugaugugcu uagcauuggg aggaguauua auauucuuaa gcacagcagu aagugcagac     2100

guaggaugca guguugacuu uaguaaa                                         2127


<210>  2507
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2507
agaggauuaa guagcagaaa aagaagaagc caugacgugu uaacaguaca auuucugaua       60

cugggaaugc uacuaaugac uggagcagua acuagaagag gaagcgcaua uuauauguac      120

uuagauagaa augaugcugg ugaagcuaua uccuuuccaa ccacauuggg aaugaauaaa      180

uguuauauac aaauuaugga cuuaggacau augugugaug cuacaauguc auaugagugu      240

ccuaugcuag augaaggagu agaaccagau gacguagauu guugguguaa uacaacguca      300

acauggguag uauauggaac uugucaucau aagaaaggag aagcaagaag aucaaggaga      360

gcugugacac uaccuucaca uagcacaaga aaacuucaaa ccagaagcca aacauggcuc      420

gaaucaagag aauauacuaa acaucuaaua cguguugaaa auuggauauu uagaaaccca      480

ggauucgcac uugccgcagc agcaauagca ugguuauugg guucgaguac aucucagaaa      540

guaauauacu uaguaaugau ucuauuaauc gcaccagcau auucaauaag augcauagga      600

gugagcaacc gagauuuugu ugaaggcaug aguggaggaa cguggguuga uauaguucug      660

gaacacggag gauguguuac aguaauggca caagacaaac caacaguaga cauagaauug      720

gucaccacaa caguaaguaa uauggcagaa guaagaagcu acuguuacga ggccaguauu      780

agugauaugg caucagauuc cagauguccu acacaaggag aagcauauuu agacaaacaa      840

ucagauacac aauauguaug uaaaaggaca uuaguugaca gaggaugggg aaauggaugc      900

ggauuauuug guaaaggauc auuagugaca ugugcuaaau uugcaugcag uaagaaaaug      960

acaggaaaaa gcauacaacc ggaaaaucuu gaguauagga uaauguuaag uguacacgga     1020

ucacaacauu cuggaaugau uguaaaugau acuggacaug aaacagauga aaaucgagca     1080

aaaguagaaa uaacucccaa uucaccaaga gcugaggcaa cacuaggagg auuugggagc     1140

uuaggauuag auugugaacc uaggacugga cuugauuuua gugaucuaua uuauuuaacg     1200

augaauaaua aacauuggcu aguacauaaa gaaugguuuc augacauccc auuaccaugg     1260

caugccgggg cagauacagg aacaccacac uggaauaaca aagaagcguu gguagaauuu     1320

aaagaugcac augcaaaaag acaaacaguu guaguauuag gaagccaaga gggagcagua     1380

cauacugcac uugcaggagc auuagaagca gaaauggaug gugcaaaagg aagacuauca     1440

ucagggcauu uaaaauguag acuuaaaaug gauaaauuaa gacuaaaagg cguaagcuau     1500

ucauuaugua cagcagcauu uacuuucaca aaaauaccag cagaaacauu acauggaaca     1560

guaacagugg aaguacaaua ugcgggaaca gauggaccau guaaaguucc agcacaaaug     1620

gcaguugaua ugcaaacauu aacaccaguu ggaagacuaa uaacagcaaa uccaguaaua     1680

acugaaucca cagaaaacuc aaaaaugaug cuugaacuag auccaccauu uggagauagu     1740

uauauaguga uuggcguugg ggagaagaaa auaacacauc auuggcauag aucaggauca     1800

acaauaggaa aagcauuuga agcaacugua agaggagcaa aaagaauggc aguucuagga     1860

gauacagcau gggauuuugg aagcguugga ggagcgcuaa auagcuuggg gaaagggauu     1920

caccaaauau uuggagcugc auuuaaauca uuauuuggag gaaugaguug guuuagccaa     1980

auacuaauag gaaccuuauu gaugugguua ggauuaaaua cuaaaaacgg aaguauaagu     2040

uuaauguguc uagcacuagg gggaguuuua auauuuuuaa guacagcagu aucagccgau     2100

guaggcugua guguagauuu uucaaaa                                         2127


<210>  2508
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2508
agaauuauaa augcaaggaa agagaaaaaa agaagaggug cagauacaag cguugguaua       60

guaggacuac uauuaacaac ugcaauggcg gcagaaguaa caagacgugg uucagcuuau      120

uacauguauu uagauagaaa cgaugcugga gaggcaauau cauuuccuac aacuuuagga      180

augaauaaau gcuauauaca aauaauggau uuaggacaua ugugugacgc aacaaugagu      240

uaugaaugcc cuaugcuaga ugaaggaguu gaacccgacg auguagauug uuggugcaau      300

acuacaagua cauggguugu auauggaaca ugucaucaca aaaaaggaga ggcuagaaga      360

ucaagaagag caguaacauu accuucacac agcacacgaa aacuacaaac aagaucacaa      420

acaugguuag aaucaagaga auacacaaaa caucuaauua ggguagaaaa uuggaucuuu      480

agaaauccug gcuuugcacu ugcagcagca gcaauagcuu gguuauuagg uagcaguaca      540

ucucaaaaag uaauauauuu aguaaugaua cuacuaaucg caccagcaua cagcauaagg      600

ugcauaggag uaucuaauag ggauuuugua gaaggaaugu caggaggaac auggguugac      660

auuguacuag aacauggugg uuguguaaca guaauggcgc aagauaaacc aacagucgau      720

auagaacuag uaacaacaac cguaucaaau auggcugaag uuagaucaua uuguuaugaa      780

gcaucaauca gcgauauggc aucagacagc agaugcccaa cucaaggaga agcuuauuua      840

gauaagcaau cagauacuca auacguaugu aaaagaacac uagucgauag aggauggggu      900

aacggaugug guuuauucgg caaaggauca uuaguuaccu gugccaaauu ugcauguuca      960

aaaaaaauga cuggaaaaag cauacaaccc gagaauuuag aauauaggau aauguugucu     1020

guccauggau cucaacacuc agguaugaua guuaaugaua caggacacga aacagaugaa     1080

aauagggcua aaguagaaau aacacccaau aguccucgag cugaagcaac acucggagga     1140

uuuggaaguc uaggacuaga cugugaacca agaacaggac uagacuuuag ugaucuauau     1200

uauuuaacaa ugaacaacaa acauuggcua guacauaaag aaugguuuca cgacauaccu     1260

cuaccuuggc acgcaggcgc ugauacagga acaccacacu ggaacaauaa agaagcacua     1320

guugaauuua aagaugcuca cgcaaaaaga caaacagucg ucguacuagg aagucaagaa     1380

ggagcuguac auaccgcgcu agccggcgcu uuagaagccg agauggacgg agcaaaagga     1440

agauuaucau cgggacacuu aaaauguaga uuaaaaaugg acaagcuaag auuaaaagga     1500

gucucauauu cacuaugcac agcagcauuu acauucacaa aaauuccugc ggaaacacua     1560

cauggaacag uaacuguuga aguacaauau gcaggaacag auggacccug caagguuccu     1620

gcacaaaugg cuguagauau gcaaacacua acaccaguag gaagacuaau aacagcaaac     1680

ccaguaauaa cagaaucuac agaaaauuca aaaaugaugu uggaauuaga uccaccauuu     1740

ggggauaguu auauaguaau agguguagga gaaaaaaaaa uaacacauca cuggcauaga     1800

aguggcucca cgauaggaaa agcauucgaa gcaacaguac gaggagcaaa aagaauggca     1860

guacuaggag acacagcaug ggauuucgga uccguuggag gagcauugaa uucauuaggu     1920

aaagguaucc accaaauauu uggagcggca uuuaaaucgu uauucggagg aaugaguugg     1980

uuuucucaaa uccuaauugg uacacuacuc augugguuag guuuaaacac aaaaaauggu     2040

ucaaucucau uaaugugcuu agcgcuugga ggaguacuaa uauuucucag uacagcugug     2100

agugcugacg uagguugu                                                   2118


<210>  2509
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2509
auuauuaaug caagaaaaga aaagaaaaga agaggugccg auacaagcgu aggaauagua       60

ggacuacuac uuacaacagc aauggcagcc gaaguaacaa gacgcgguuc ugcuuauuau      120

auguaucuag acagaaauga ugcuggagaa gccauuaguu ucccaacaac auuaggaaug      180

aauaaauguu acauacaaau aauggaccua ggacacaugu gugacgcaac aaugucauau      240

gaaugcccaa uguuagacga agguguugaa ccagacgaug uagauuguug guguaauaca      300

acaucaacau ggguuguuua uggaacaugc caucauaaga aaggcgaggc uagaagauca      360

agacgagcag ucacauuacc uagucauagu acaagaaaau uacaaacaag aucucagaca      420

uggcuugaau caagagaaua uacaaaacac uuaauuagag uugaaaauug gaucuuuaga      480

aacccugguu uugcacuagc cgcagcugca auagcuuggc uauuaggauc aucuacauca      540

caaaaaguaa uauauuuagu caugauucua cuaauagcac cagcauauuc aaucagaugc      600

auaggaguga gcaauagaga uuuuguggaa ggcaugucug gagggacaug gguagauauu      660

guauuagaac auggagggug cguaacuguu auggcacagg auaaaccaac aguagacaua      720

gaauuaguua caacaacagu uucaaauaug gcggaaguua gaaguuacug uuaugaagca      780

ucaauuucag auauggcauc agauaguaga uguccaacac aaggggaggc uuauuuagau      840

aaacaaagug acacucaaua uguauguaaa cgaacauuag uagauagagg auggggcaac      900

gguugugguu uauuuggcaa aggaaguuua guaacaugug caaaauuugc guguaguaag      960

aaaaugacag gcaaaucaau acaaccagaa aacuuagaau aucgaaucau gcuuagugua     1020

cacggcucac aacauucagg aaugauugua aaugacacug gacaugagac agaugaaaac     1080

agagcaaaag uggaaauuac accuaacuca ccaagagcag aagcuacguu aggagguuuc     1140

ggaucucuug gauuagacug ugaaccaaga acaggcuuag acuucucuga ucuauauuau     1200

cuuacaauga auaacaaaca uugguuagua cauaaagagu gguuucauga uauaccauua     1260

ccauggcacg caggagcaga uacagggacg ccacauugga acaauaaaga agcucuagua     1320

gaauuuaaag augcacacgc aaaaagacaa acaguaguug uuuuagguuc ucaagaagga     1380

gccguacaca cagcacuagc uggagccuua gaagcagaaa uggacggggc uaaagguaga     1440

uuaucaucug gacacuuaaa auguagauua aaaauggaua aauuaagauu aaaaggagua     1500

aguuauagcc uguguacagc cgcauuuacu uuuaccaaaa uaccagcaga aacauuacau     1560

gguacaguaa ccguagaagu gcaguaugca ggaacagaug ggccuuguaa agucccagcu     1620

caaauggcag uagauaugca aacacuuacc ccaguaggca gacuuauaac ugcaaaucca     1680

gugauaacag aaucaaccga gaacuccaaa augaugcuag aacuagaucc accauucgga     1740

gauaguuaua uaguuaucgg agucggagaa aagaaaauaa cucaucauug gcauagauca     1800

gguagcacga uaggaaaagc auuugaagca acagucagag gugccaaaag aauggcagua     1860

uugggagaua cagcauggga cuuuggaagu gucggaggag cacuaaauag cuuaggaaaa     1920

ggaauacauc aaauuuucgg agcagcauuu aaaucuuuau uuggaggaau gagcugguuc     1980

ucacaaauac uuauaggaac auugcugaug ugguuaggac ucaauaccaa aaacggaagc     2040

auaucauuaa ugugucuagc acugggaggu gucuuaauau uccuaucgac agcaguauca     2100

gcagaugugg gaugu                                                      2115


<210>  2510
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2510
aucaacgcaa gaaaagaaaa aaaaagacga ggagcagaca caaguguagg aauaguagga       60

uuacuuuuga caacugcaau ggcagcggaa guaacaagaa gaggaagugc auauuauaug      120

uauuuagacc gcaaugaugc cggugaagcg auaucauucc caacuacuuu aggaaugaau      180

aaauguuaua uacaaauaau ggauuuagga cauaugugug augcaacgau gagcuaugaa      240

ugccccaugu uagaugaagg aguagaacca gacgauguag acuguuggug uaauacaaca      300

aguacauggg uaguauaugg aacgugucau cacaaaaaag gagaagcaag gagaaguagg      360

agagcaguaa ccuuaccauc ccauagcacu cgaaaauuac aaacaagaag ccaaacaugg      420

uuagaaaguc gagaauauac aaaacauuua auaagagucg aaaauuggau auuuagaaau      480

ccaggauucg cauuagcagc agcugcaaua gcauggcuau uagguucauc aacaagucaa      540

aaaguaauau auuuaguaau gauuuuacua auagcaccag cauauucaau aagauguauu      600

ggaguaagca accgcgauuu uguagaagga augucaggag gaacaugggu agacauagua      660

cuagaacaug gagggugugu cacaguaaug gcacaagaua aaccaacggu agauauagaa      720

uuaguaacga ccaccguuuc uaacauggcu gaaguacgau cauauugcua cgaagcauca      780

auaagcgaca uggcaucaga uucuagaugu ccaacucaag gagaagcgua uuuggauaaa      840

caauccgaua cacaauacgu augcaagagg acucuaguag auagggguug gggaaacgga      900

uguggguuau uuggaaaagg aaguuuaguu acaugugcua aguuugcgug uaguaaaaaa      960

augacaggaa agucaauuca accagaaaau uuggaauaca gaauaauguu aagcguacau     1020

ggcucacaac acagcggaau gauagugaau gacacaggac augaaacaga ugaaaauaga     1080

gcaaaaguag aaauaacacc aaauagucca agagcagaag cgacacuagg agguuuuggu     1140

ucauuagguc uagauugcga accucgaaca gguuuagacu uuucagaucu guauuauuua     1200

acaaugaaca auaaacauug guuaguccau aaagaauggu uucacgauau accuuuaccg     1260

uggcacgccg gagcagacac aggaacaccc cauuggaaua auaaagaagc acuaguagag     1320

uuuaaagacg cucaugcaaa aagacaaacu guaguaguuu uggguagcca agaaggagcc     1380

guacauacag cauuagcagg agcgcuagaa gcagagaugg acggcgcuaa aggaagauua     1440

uccuccggac auuuaaaaug ucgauuaaaa auggauaaau uaagacuaaa aggaguauca     1500

uauaguuuau gcaccgcagc uuuuacauuu acaaaaauac cugcagaaac ucuacaugga     1560

accguaacag uagaaguuca auaugcaggc acagauggac cauguaaagu uccugcacaa     1620

auggcuguag auaugcaaac acucacaccc guuggaagau uaauaacagc gaacccagua     1680

auaacugaaa gcacagaaaa uaguaaaaug auguuagaau uagacccacc auucggagau     1740

aguuauauag uaauaggugu aggagaaaaa aaaauaacuc accauuggca cagaucaggg     1800

ucaacuauug gaaaagcauu ugaggcaaca guaagaggag cgaaacguau ggcaguauua     1860

ggagacaccg cgugggacuu ugguagcgua ggaggagcau uaaacucguu aggaaaagga     1920

auccaucaga uauucggagc agccuuuaaa agucucuucg gaggaaugag cugguucuca     1980

caaauuuuaa uaggaacauu acuaaugugg cuaggauuaa auacaaaaaa uggcaguaua     2040

ucauuaaugu gcuuagcucu aggaggagua cuaauauucu uaaguacugc agucucagcu     2100

gaugugggau gc                                                         2112


<210>  2511
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2511
aacgcaagaa aagaaaaaaa acgucguggu gccgauacaa guguaggaau aguaggauua       60

uuguuaacaa cagcaauggc agcagaagua acacgaagag gaucagcaua uuacauguau      120

uuggauagaa augaugcggg agaagcaaua ucauuuccaa cuacacuugg aaugaauaaa      180

uguuauauuc aaauuaugga uuuaggacau augugcgacg cuacaauguc auacgaaugc      240

ccgauguuag acgaaggggu ggaaccagau gacgucgauu guugguguaa uacaacguca      300

acguggguag uauauggaac augccaucac aaaaaaggcg aagcuagaag aagucguaga      360

gcaguaacau uaccaagcca uaguacaaga aaauuacaaa cacgauccca aacuugguua      420

gaaucaaggg aauacacuaa acauuugaua agaguagaaa auuggauauu uagaaauccg      480

gguuucgccc uugcggcagc agcaauagca ugguuacuag gaucuucaac aagucaaaaa      540

guaauauauu uaguaaugau acuuuuaaua gcaccagcuu auagcauaag augcauuggu      600

guaaguaaua gggacuuugu agaagguaug ucagguggaa cauggguaga uauaguacua      660

gaacauggag guugcguaac aguuauggca caagauaaac caacggucga cauagaacua      720

guuacaacaa caguuagcaa uauggcagaa guaagaucau auuguuacga agcaucuaua      780

agcgacaugg caagugauuc uagaugucca acacaaggag aggcauaccu ugauaaacaa      840

agugauacuc aauauguaug caaaagaaca uuaguagauc gaggaugggg aaacgggugu      900

ggucuauuug guaaaggauc auuaguaacg ugugcaaaau uugcauguuc gaaaaaaaug      960

acaggaaaau caauacaacc agaaaacuua gaauauagaa uaaugcucag cguacauggu     1020

ucacaacauu caggaaugau uguaaaugau acaggucaug aaacagauga aaaccgagca     1080

aaaguagaaa uuacuccaaa uaguccaaga gcugaagcaa cacuuggagg uuuuggaagu     1140

cuaggacuag acugcgaacc aagaacugga uuagauuuuu cugaucuaua cuaucuaaca     1200

augaacaaua agcauugguu aguacauaaa gagugguuuc augauauucc acuaccuugg     1260

caugcaggug cugauacagg aaccccacau uggaauaaua aagaagcuuu gguagaauuu     1320

aaagaugcac augcaaaaag acaaacugua guaguauuag gaucacaaga aggagcagua     1380

cacaccgcac uagcaggagc uuuagaagcu gaaauggaug gagccaaagg aagauuaucc     1440

aguggacauu uaaaaugcag auuaaagaug gacaaacuac gacuaaaagg aguaagcuau     1500

ucauuaugua cagcagcauu uacauuuacc aaaauaccug cugaaacucu acacggaaca     1560

guaacaguag aaguacaaua ugcggguaca gauggaccau guaaaguacc agcucaaaug     1620

gcuguagaua ugcagacuuu aacaccggua ggaagauuga uaacugcaaa uccuguaaua     1680

acugaaucaa cagaaaauag uaaaaugaug uuagaauuag auccaccauu cggagauuca     1740

uacauaguaa uuggugucgg ggaaaaaaaa auaacccauc auuggcauag aucaggaagu     1800

acaauaggaa aagcuuuuga agcaacagua agaggagcaa aaagaauggc ggugcucgga     1860

gauacagcuu gggauuuugg caguguaggu ggugcacuaa auucauuagg uaaaggaaua     1920

caucaaauuu uuggagcugc guuuaaauca cuauucggag gaaugucuug guuuucucaa     1980

auauuaauug ggacuuuguu aaugugguua ggucuaaaua caaaaaaugg gucaauauca     2040

cuaauguguu uagcacuggg aggaguauua auuuuuuuaa guacagcagu aagcgcagac     2100

guaggaugu                                                             2109


<210>  2512
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2512
gccagaaaag aaaaaaaaag aagaggagcu gauacaucag uaggaauagu aggacuauug       60

uugacaacag caauggcagc agagguaaca agacgaggua gcgcauauua cauguauuua      120

gauagaaacg augcaggaga agcuauauca uucccaacua cauuaggaau gaacaaaugc      180

uauauacaaa uuauggauuu aggacauaug ugugaugcaa caaugaguua ugaaugcccu      240

auguuagaug aaggaguaga acccgacgau guugacugcu ggugcaauac gacaaguaca      300

uggguuguau augguacaug ucaucauaaa aaaggagaag caagacgaag uagacgcgca      360

gucacauuac cgagccauag cacgcguaaa cuacaaacac gaucacaaac uuggcuagag      420

aguagagaau auacuaaaca uuuaaucaga gucgaaaacu ggauauucag gaaucccgga      480

uuugcauuag cagcagcagc aaucgcaugg cuauuaggcu caaguacuag ucaaaaagua      540

auauauuuag uaaugauauu acuaauugcc ccagcauaca guauaagaug uauaggugua      600

agcaaccgag acuucguaga aggaaugucu ggaggaacau ggguugacau uguacuagaa      660

cacggagguu gugucacagu gauggcgcaa gauaaaccua caguugauau ugaacuaguu      720

acaacaacag uaaguaacau ggcugaagua agaucauacu guuaugaagc aucuauaagu      780

gauauggcua gugacagucg auguccaaca caaggcgaag cauacuuaga uaaacaauca      840

gauacucaau auguauguaa aagaacacua gucgacagag gguggggaaa cggaugugga      900

cuauuuggaa aaggaucgcu agugacaugu gcaaaauuug caugcucaaa aaaaaugaca      960

ggcaaaucaa uacaaccuga aaauuuagaa uaccggauca uguuaucagu ccaugguuca     1020

caacauagcg gaaugauagu caaugauaca ggacaugaaa cggaugaaaa cagggcaaaa     1080

guagaaauaa cuccaaacag uccaagagca gaagccacau ugggaggcuu uggaagcuua     1140

ggauuagacu gcgaaccgcg uacaggcuua gauuucuccg aucuuuauua ucuaacuaug     1200

aauaauaaac auugguuggu acauaaagaa ugguuucacg acaucccauu accuuggcau     1260

gcaggagcag auacaggaac accacauugg aacaauaagg aagcauuagu agaauucaaa     1320

gacgcacaug caaaaagaca aacaguagua guacuaggau cacaagaagg agcaguccau     1380

acugcucucg caggagcguu agaagcagaa auggacggag caaaagggcg uuugagcuca     1440

ggacauuuaa aaugcagauu aaaaauggau aaauuacgau uaaaaggagu aucuuacucg     1500

cuauguaccg cagcuuucac auucacaaaa auaccagcag aaaccuuaca ugggacagua     1560

acgguugaag ugcaauaugc agggacagau ggcccaugua aaguacccgc ucaaauggca     1620

guugacaugc aaacucuaac accaguagga agauuaauua cagcaaaccc aguaauuaca     1680

gaaaguacug aaaacaguaa aaugauguug gaacuagauc caccauuugg agacagcuau     1740

auaguuaucg gcguagguga aaaaaaaaua acacaucauu ggcauagaag ugguaguacg     1800

auaggaaaag cuuucgaagc uacgguaaga ggagcaaaaa gaauggcagu cuugggagau     1860

accgcauggg auuuugguag uguugguggu gcauuaaauu cauuagguaa aggaauacac     1920

caaauauuug gagcagcauu caaaucuuua uucggaggua ugaguugguu uucacaaaua     1980

uugauaggaa cauuacuaau guggcuaggc uuaaauacaa aaaaugguuc cauaaguuua     2040

auguguuuag cauuaggggg uguguuaaua uuuuuaucua cugcaguuag ugcugaugua     2100

ggcugu                                                                2106


<210>  2513
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2513
agaaaagaaa agaaaagaag aggugcagac acuucaguag gaauuguggg auugcuacuu       60

acaacugcaa uggcagcaga aguaacacga cguggaucug cauacuauau guacuuagac      120

aggaaugacg cuggugaagc aauaaguuuu ccaacaacau uaggcaugaa caaaugcuac      180

auacaaauaa uggaccuagg acauaugugu gacgcaacca ugaguuacga auguccaaug      240

uuagaugaag gaguagagcc cgaugaugua gauugcuggu guaauacaac aagcacaugg      300

guaguguaug gaacauguca ccauaagaaa ggugaagcaa gaagaucaag gagagcagua      360

acucuuccau cacauucaac gcgaaaauua caaacaaggu cacaaacaug guuagaaagu      420

agagaauaua cgaaacaccu cauaagagua gaaaauugga uauucagaaa ucccggcuuu      480

gcacuagcag cagcagcaau agcaugguua cugggaagua guacaucaca gaaaguaaua      540

uauuugguaa ugauauuacu uauagcccca gcauauucaa uuagauguau aggaguuucg      600

aauagagauu ucguggaagg caugagugga ggaacguggg uagacauagu uuuagaacau      660

ggaggaugug ucacagugau ggcacaagau aaaccgacag uagauauaga auuagugaca      720

acuacaguaa guaauauggc agaaguuaga aguuauuguu augaagcauc cauaagcgau      780

auggcuucag auucaagaug uccgacucaa ggagaagcau auuuagauaa acaaagugac      840

acacaauacg uauguaaaag gacucuagua gauagaggau gggguaaugg auguggguua      900

uuugguaaag gaucuuuagu cacaugugca aaauucgcau gcaguaagaa aaugacaggc      960

aaaaguaucc agccagaaaa uuuggaauau aggauaaugc uaucaguaca cgguucucaa     1020

cauuccggaa ugauugucaa ugauacaggu caugaaacag augaaaacag ggcgaaagug     1080

gagauuacac caaacucacc aagagcggaa gcaacacuag guggauuugg uucauuggga     1140

uuagauugcg aaccaagaac aggauuagau uuuucagauc uguacuauuu aacuaugaac     1200

aacaaacauu gguuaguaca uaaagaaugg uuucaugaua uaccauuacc auggcaugcg     1260

ggagcagaua caggaacgcc acacuggaac aacaaagaag cacucguaga auuuaaagac     1320

gcacaugcga aacgacaaac aguaguagua uuaggaagcc aggaaggagc aguacauaca     1380

gcacuagcag gcgcauuaga agcagaaaug gauggagcaa aagguagauu aaguagugga     1440

cauuuaaaau guagguuaaa aauggauaaa cuaagauuaa aaggaguaag uuauaguuua     1500

ugcacugccg cauucacauu uaccaaaaua ccugcagaga cauuacaugg cacaguaaca     1560

guugaaguac aauacgcagg cacagauggg ccaugcaaag ugcccgcuca aauggcagua     1620

gauaugcaaa ccuuaacgcc aguuggcaga uuaauaacug caaacccagu uauaacugaa     1680

ucgacagaaa acagcaaaau gauguuagaa cuagauccgc cuuuuggaga uucauauaua     1740

guaauuggag uaggagaaaa gaaaauaaca caucacuggc auagaagugg aagcacaaua     1800

ggaaaagcau uugaagcgac aguaagggga gcgaaaagaa uggccguauu aggagauaca     1860

gcaugggacu ucgguagugu aggaggagcu uuaaauaguc uaggaaaagg aauacaucaa     1920

auauuuggag cagcuuuuaa aucacuauuu ggugguaugu caugguuuuc acaaauauua     1980

auaggaacau uacuaaugug gcuaggccua aauaccaaaa auggaaguau aaguuuaaug     2040

uguuuagcau uagguggugu auuaauauuu cuaaguaccg caguaucagc agauguugga     2100

ugu                                                                   2103


<210>  2514
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2514
aaagaaaaga aaagaagagg ugcagauaca uccguaggca uaguagguuu guuauuaaca       60

acagcaaugg cugccgaagu gacuagacgu ggaagugcau acuacaugua uuuagauaga      120

aaugaugcug gagaagcuau uucauuuccu accacauuag gaaugaauaa auguuacaua      180

caaauaaugg aucuagguca caugugcgau gccacaauga gcuacgaaug uccaauguua      240

gaugagggag uagaaccaga cgauguugau uguuggugua auacaacauc aacgugggua      300

guuuauggua caugccauca uaagaaagga gaagcuagaa gaucaagacg agcaguaaca      360

uuaccaaguc acucaacuag aaaacuacaa acaagaagcc aaacauggcu agaaagcaga      420

gaauauacua aacaccuuau uagaguagaa aauuggauau uuaggaaccc aggauuugcu      480

uuagcggcug cugcaauugc auggcugcuu ggaucaagua caucucagaa agucauauau      540

cugguaauga ucuuauuaau agccccugca uacaguauca ggugcauagg aguaucgaau      600

cgugauuucg uagaaggaau gagcggggga acauggguug acauaguauu agagcaugga      660

ggauguguua caguaauggc acaagauaaa ccaacugucg auauagaauu aguaaccacc      720

acaguaagca acauggcaga aguaagaagu uauuguuaug aagcaucaau aagcgauaug      780

gcaucugacu cacgaugucc aacacaaggg gaagcauauc ucgauaaaca gucagauacu      840

caauauguuu guaaaagaac ucuaguagau cgaggcuggg gaaacggaug cggucucuuc      900

ggaaaaggau cauuaguaac augugcaaag uucgcaugua guaagaaaau gacaggaaaa      960

uccauacaac cagaaaauuu agaauauaga auuauguuaa gcguacaugg uucucaacac     1020

ucaggaauga uaguaaauga uacaggccau gaaacagacg aaaacagagc caaaguagag     1080

auaacaccaa acaguccacg agcugaagcu acauuaggag gguucggauc auuaggacug     1140

gauugugaac ccagaacugg ucuagacuuu ucagaccuuu acuauuuaac aaugaauaau     1200

aaacauuggc uaguacacaa agaaugguuu cacgacauuc cguuaccaug gcaugcgggu     1260

gcagauacag gcacaccaca uuggaauaau aaagaagcuc uaguagaguu uaaagaugca     1320

caugcaaaac gacaaacagu cguaguacua gguagucaag aaggagcagu acauacugca     1380

uuagcuggag cauuagaagc agaaauggau ggagcaaaag gaaggcuauc auccggucau     1440

cuaaaaugua gacuaaaaau ggauaaacug agauuaaagg gagucagcua uucauuaugu     1500

acagcagcau ucacauucac caaaauccca gcagagacuu uacauggaac aguaacaguc     1560

gaaguacaau augcagguac agauggacca uguaaaguuc cagcacaaau ggcuguagau     1620

augcaaacgu uaacacccgu ugguagacua auaacagcua acccaguuau uacagaaagc     1680

acagaaaauu caaaaaugau guuagagcua gacccaccau ucggugauag cuauauuguc     1740

auaggagucg gagaaaagaa aauaacacac cauuggcaca gaucaggauc aacaauagga     1800

aaagccuuug aagcuacagu aagaggagca aaacgaaugg ccguauuagg ugauacagca     1860

ugggauuuug gaucaguugg aggagcacuu aacucucuag guaaaggaau acaucaaaua     1920

uuuggagcag cuuucaaauc ucuauuuggu gguaugaguu gguuuaguca aauauuaauu     1980

gguacauuac uaaugugguu aggucuaaau acuaaaaaug guucaauaag uuuaaugugu     2040

uuagcauuag gaggcguuuu aauauuuuua aguacagcag uaagcgcuga cguuggcugu     2100


<210>  2515
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2515
gaaaagaaaa gaagaggagc agauacaagu guaggaauug uuggacuauu auuaacuaca       60

gccauggcag cagaaguaac gagaaggggu agugcuuauu acauguaucu agacaggaau      120

gaugcagggg aagcaauauc uuucccaacu acauuaggca ugaauaaaug uuauauucaa      180

auaauggauc uaggacauau gugugaugca acaaugucau augaaugccc aauguuagau      240

gagggugugg aacccgauga uguagauugc ugguguaaua caacaagcac auggguugua      300

uauggaaccu gccaucauaa gaaaggagaa gcaagaagau cuaggagagc agugacguua      360

ccaagucauu ccacaagaaa auuacaaaca agaucgcaga caugguuaga aagcagagaa      420

uacacaaaac acuuaauaag aguagaaaau uggauuuuca gaaauccagg uuuugcauua      480

gcagccgcag caauagcaug gcuccuagga ucaaguacau cucaaaaagu uauauauuua      540

gucaugauac uacuaauugc uccugcauau aguauaagau gcauaggugu uaguaauaga      600

gauuuuguag aagggauguc aggagguaca uggguagaua uaguauuaga acauggagga      660

uguguaacug uaauggcaca agacaaaccu acaguagaua uagaacucgu aacuacaacc      720

guaaguaaua uggcagaggu aagaucauau uguuacgaag caucaauauc agauauggca      780

ucagauagua gaugcccuac gcaaggagaa gcauaucuag auaaacaaag cgauacucaa      840

uauguaugua aacgaacuuu aguagauaga ggguggggaa auggaugugg acuauuuggu      900

aaaggaucuu uaguaacuug ugcaaaauuu gcuuguucaa agaaaaugac uggaaaauca      960

auucaaccag aaaauuuaga auaucgaaua auguuaucag uacauggauc gcaacacagc     1020

ggaaugauag uaaacgauac cggacacgaa acugacgaaa acagagcaaa aguagagaua     1080

acacccaaua guccaagagc agaagcuaca cuaggaggau uuggcagccu uggacucgac     1140

ugugagccaa gaacgggauu agacuuuagc gaucuauauu acuuaacaau gaauaacaaa     1200

cauugguuag uccauaaaga augguuucau gacauuccac uaccauggca cgcuggugca     1260

gauacuggaa caccgcacug gaauaauaaa gaagcccuag uugaauuuaa agaugcacau     1320

gcaaaaagac aaacagucgu aguauuaggu ucucaggaag gagcaguuca uacagcauua     1380

gccggugcau uagaagcaga aauggaugga gcaaaaggaa gauuaaguuc uggacaucug     1440

aaaugucgac uaaaaaugga caaacuaaga uuaaaaggag uaucauacuc auuauguaca     1500

gcagcauuua cauucacuaa aauaccagca gaaacauugc acgguacagu aacaguagaa     1560

gugcaauaug cuggaacuga uggcccgugu aaaguaccgg cacaaauggc uguagauaug     1620

cagacauuaa caccaguagg aagauuaaua acagcaaauc cuguaauaac agaaagcaca     1680

gaaaacagua agaugauguu agaauuagac ccacccuucg gggacagcua uauaguuaua     1740

ggaguuggag aaaagaaaau aacacaucac uggcauaggu caggaucaac aauagggaaa     1800

gcauucgaag cgacuguuag aggagcaaaa agaauggcgg uauugggaga uacugcaugg     1860

gauuucggau caguaggagg agcauugaau agucuaggca aagguauaca ccaaauauuc     1920

ggggcagcau uuaaauccuu auuuggugga augaguuggu uuagucaaau uuuaauagga     1980

acuuuacuga ugugguuagg acuaaauacu aaaaauggau ccaucucauu aaugugucuc     2040

gcgcuaggag gaguacuaau uuucuuaucu acagcaguau cagcggacgu aggaugc        2097


<210>  2516
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2516
aaaaaaagaa ggggagcuga uacaagcguc ggaauaguag gacuauuauu aacaacagca       60

auggcagcag aaguaacacg aagaggcagc gcuuauuaua uguauuuaga uagaaaugau      120

gcaggugaag ccauaucauu uccaacaacu cuaggaauga auaaauguua cauacaaaua      180

auggauuuag gacauaugug ugaugcaaca augaguuaug aaugcccaau gcuggaugaa      240

gguguugaac cugaugaugu agauuguugg uguaauacaa caucaacaug gguaguauac      300

ggaacaugcc aucacaagaa aggagaagca agaagaucaa gaagagcagu aacacuacca      360

agucacucua caagaaaauu acaaacaaga agucaaacgu gguuagaauc aagagaauau      420

acaaaacacu ugauacgagu agaaaacugg aucuuuagaa auccaggauu cgcccuugca      480

gccgcugcaa uugcaugguu acuagguagu aguacuaguc aaaaaguuau auaccuuguc      540

augauauuau uaauagcucc agcauacuca auaagaugca uaggcguaag uaauagagau      600

uuugucgaag gaauguccgg aggaacaugg guugacauag uauuagaaca cggaggaugu      660

guaacaguaa uggcacaaga uaaaccaacu guugauauug aguuaguuac aacuacagua      720

ucaaauaugg cagagguacg aaguuauugu uacgaggcaa guauaucuga uauggcaucu      780

gauucuagau guccaacuca aggagaagca uaucuugaca aacaaucuga uacacaauau      840

gucuguaaaa gaacauuagu agaucgugga ugggggaacg gauguggauu auucggaaaa      900

ggaucacuag uaaccugugc uaaauuugca uguuccaaaa aaaugacugg uaaaucaauc      960

caaccagaaa auuuagaaua caggauaaug uuaucagucc acgguaguca acauagcggu     1020

augauaguua augauacagg acaugaaaca gaugaaaauc gagcaaaagu ugaaauaacc     1080

ccaaauucac caagagcaga agccacacua gggggcuuug gaucauuggg auuagacugc     1140

gaaccucgaa caggauugga cuucucugau uuauauuauc uaacgaugaa caauaaacac     1200

ugguuaguac acaaagaaug guuucacgau auaccucuac cauggcaugc aggugcagac     1260

acaggaacac cacauuggaa caauaaagag gcacuaguag aauucaaaga ugcgcacgcc     1320

aaacgacaaa cagucguagu uuuagguuca caagaaggag cuguacauac agcuuuagca     1380

ggggcguuag aagcagaaau ggauggugcg aaaggacguu ugucaucugg ucauuuaaaa     1440

ugucgauuga aaauggauaa acuacgacua aaaggaguaa gcuacucacu uuguacugcg     1500

gcuuuuacau uuacaaaaau accagcagaa acccugcaug gaacuguuac uguagaagua     1560

caauaugcug gcacagacgg accuuguaaa guuccagccc aaauggcagu agauaugcaa     1620

acacuaacuc caguaggaag acuuauaaca gcaaauccag ugauuacaga aucaacagaa     1680

aauaguaaaa ugauguuaga acucgaucca ccauuuggag auagcuacau agucauagga     1740

guaggcgaaa aaaaaauaac acaccauugg cauagaagcg gaaguaccau aggaaaggcc     1800

uuugaagcga caguaagagg agcuaaacga auggcaguac uaggggauac ggcaugggac     1860

uuugguagcg uaggaggagc auuaaacucu cucggaaaag gaauacacca aaucuuugga     1920

gcugcauuua aaucauuauu ugguggcaug aguugguuua gccaaauccu aauaggaaca     1980

uuauuaaugu gguuagguuu aaacacaaaa aauggaucca uuucauugau gugucuggca     2040

cuuggaggag uauuaaucuu ucuuuccacu gcaguaagcg cagauguagg augu           2094


<210>  2517
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2517
aagcgaagag gagcagauac uucaguagga auaguaggac uauuauuaac aacagcaaug       60

gccgcagaag ucacaagaag aggaucagca uacuauaugu accuagauag aaacgaugcu      120

ggagaagcaa uaucguuccc aacaacacua ggaaugaaua aauguuauau ucagauaaug      180

gaucucggac auaugugcga ugcaacaaug ucauaugaau guccaauguu agaugaagga      240

gucgaaccug augauguaga uuguuggugu aauacaacau caacaugggu aguauaugga      300

acaugucauc acaaaaaggg cgaggcuaga agaucccgua gagccgucac auuaccaucg      360

cauaguacaa gaaagcuaca aacacguucu cagacauggc uagaaaguag agaauauaca      420

aaacauuuaa uccgagucga aaacuggaua uuccgcaauc caggguuugc acuagcugcc      480

gcagcuauug cauggcugcu agguucauca acgucacaaa aaguuauaua ccuagugaug      540

auauuauuaa ucgcaccagc auauaguauu agauguauag guguaaguaa uagagacuuu      600

guagaaggua ugagcggagg aacauggguc gauauaguau uagaacaugg cggaugugug      660

accguaaugg cacaagauaa acccacggua gauauagaau uggucacaac uacuguauca      720

aauauggcag aaguaagauc auacugcuac gaagcaucaa ucucagauau ggccucagau      780

uccagauguc cgacccaagg agaagcauau cuagacaaac aaucggauac acaauaugua      840

uguaaacgaa cgcuaguuga uagaggaugg ggaaauggau guggauuauu uggaaaagga      900

aguuuaguca caugugcaaa auucgcaugc agcaagaaaa ugaccggaaa auccauacaa      960

ccagaaaauc uagaauauag aauaaugcua ucaguacacg gaagccagca uaguggaaug     1020

auuguaaaug auaccggaca ugaaacugac gaaaauagag caaaaguaga aauaacacca     1080

aauaguccaa gagcagaagc aacauuaggg ggauucggau cacuagguuu agacugcgaa     1140

ccacgaaccg gauuagauuu cagugacuua uacuauuuaa caaugaauaa uaaacauugg     1200

uuaguacaca aagaaugguu ucaugacaua ccauuaccau ggcaugcagg agcagacaca     1260

ggaacaccac acuggaacaa uaaagaagca uuaguagaau uuaaagaugc acaugcaaaa     1320

cggcaaacag uaguaguauu aggaagucaa gaaggcgcug uacacacagc acuagcugga     1380

gcauuggaag cagaaaugga cggagcaaaa ggaagguuau caaguggaca cuuaaaaugu     1440

agauuaaaga uggauaaacu aagacuaaaa ggaguaaguu auucauuaug cacagcugcu     1500

uuuacauuua cuaaaauccc agcagaaaca cugcacggga caguaacagu ugaaguacaa     1560

uaugcaggua cugauggacc auguaaagua ccugcucaaa uggcaguuga uaugcaaaca     1620

uuaacaccag uagggagauu aauuacugca aacccaguaa uaacagaauc uacugaaaau     1680

ucaaaaauga uguuagaauu agauccaccg uuuggagaua guuauauagu aauaggaguu     1740

ggagagaaaa agauaacaca ucauuggcac agaagcggau caacuauagg aaaagcauuu     1800

gaagccacag uaagaggagc aaaaagaaug gcaguguuag gagauacagc augggauuuu     1860

ggaucagugg gaggugcacu uaauucauua ggaaaaggua uucaccaaau auucggagca     1920

gccuuuaaaa gucuuuucgg ggguaugagu ugguucagcc aaauacuaau aggaacacua     1980

cuuauguggu uaggauuaaa uacuaaaaau gguaguauau cuuuaaugug uuuagcauua     2040

ggcggaguac ugauauuuuu aucaacagcg guuagcgcug acguggguug c              2091


<210>  2518
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2518
aggcguggcg cagauacauc aguagggauu guaggauuau uauuaacaac agcaauggca       60

gcagaaguua caagaagagg aagugcauac uacauguauu uagauagaaa cgaugccgga      120

gaagcaauau ccuuuccaac aacauuagga augaauaaau guuauauaca aauaauggac      180

uuaggacaca ugugugacgc aacuauguca uaugaaugcc caaugcuaga ugaaggaguc      240

gaaccugaug acguagauug cugguguaau acaacaagua cauggguagu auauggaaca      300

ugucaucaua agaaaggcga agcaagacga ucaaggagag caguuacacu gcccucacac      360

ucaacaagaa aacuacaaac aagaucacaa acuugguuag aaucuagaga auauacaaaa      420

caucuuauaa gagucgaaaa uuggauauuu agaaacccag gauuugcauu agccgcagca      480

gcaauagcau gguuauuagg gaguucaaca ucacagaaag uuauauauuu aguaaugaua      540

uuauuaauug caccagcaua cucaauaaga uguauaggag uuucgaauag ggauuucgua      600

gaaggaaugu cgggagguac cuggguagau auaguuuuag aacauggcgg uuguguaaca      660

guuauggcac aagauaaacc uacaguagau auagaacuug uaacaacaac aguuagcaac      720

auggcugaag uaagaagcua uuguuacgaa gcaucaauau cagacauggc auccgauuca      780

agaugcccga cacaaggaga agcauaucua gauaaacaau cagauacaca auauguaugc      840

aaacgaacuc uaguagacag aggcugggga aacgguugcg gacuauucgg aaaagguagc      900

cuaguaaccu gugcuaaauu cgcauguuca aagaaaauga cuggaaaauc aauucaaccu      960

gaaaacuuag aauauagaau uauguuaagc gugcauggaa gccaacauuc agguaugauu     1020

gucaaugaua cgggacauga aacagaugaa aaccgugcaa aaguagaaau aacaccaaau     1080

uccccgaggg cagaagcaac acuaggggga uuuggaucac uaggcuuaga uugugaaccu     1140

agaacuggau uagauuuuag ugaucuauau uaccuaacaa ugaacaauaa acauugguua     1200

guucauaaag aaugguuuca ugauauucca uuaccguggc acgccggugc agauacaggu     1260

acgccacacu ggaauaacaa agaagcauua guagaauuua aagaugcaca ugcuaaacgu     1320

caaacugucg uaguauuagg uucacaagaa ggagcaguac auacagcauu agcaggagcc     1380

cuagaagcgg aaauggaugg agcaaaagga cgccuaucaa guggacacuu aaaaugucgc     1440

uuaaaaaugg acaagcuaag auuaaaagga gucaguuauu cucuaugcac agccgcuuuc     1500

acauucacga aaaucccugc ggaaacacuu cacggaacag uaacuguaga aguucaauac     1560

gcagguacag acggcccgug uaaaguacca gcccaaaugg ccguagauau gcaaacauua     1620

acaccaguag gaagacuaau aacugcaaau ccaguuauaa cagaaucgac agaaaacagc     1680

aaaaugaugu uagaacuaga cccaccauuu ggagauucuu acaucguuau cggaguagga     1740

gaaaagaaaa uuacacacca cuggcauaga aguggaucaa caauuggaaa agcauucgaa     1800

gcaacgguaa gaggagccaa acgaauggca guuuuaggag acacugcaug ggacuucgga     1860

agcguaggag gugcauuaaa cucguuaggg aaaggcauuc aucagauauu uggagcagca     1920

uucaaaucau uauucggagg uaugucaugg uuuagucaaa uacuaauugg aacuuuacua     1980

augugguugg guuuaaacac uaaaaacggu aguauaucac uuauguguuu agccuuaggc     2040

ggaguguuaa uauuuuuauc aacagcagua agcgcugaug uagguugu                  2088


<210>  2519
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2519
agaggagcug auacaucagu aggaauugua gguuuauuac uaacaacagc aauggcagca       60

gaaguuacaa gaagaggauc agccuauuau auguauuuag aucgaaacga ugccggagaa      120

gcaauaaguu uuccaacaac uuuaggaaug aauaaaugcu auauacaaau cauggaucuu      180

ggacacaugu gugacgcaac aauguccuac gaauguccaa uguuagacga aggaguagaa      240

ccugacgacg ucgauuguug guguaauacc acaucaacau ggguaguuua cgguacaugc      300

caucauaaga aaggagaagc aaggcgauca aggagagcag uaacauuacc cucacauagu      360

acaagaaaau uacagacuag aucacaaaca uggcuugaau cgagagaaua cacuaaacac      420

cuaauaagag uagaaaacug gauuuuuaga aauccaggau uugccuuagc agcagcagcg      480

auagcauggc uacuuggguc uaguacaagu caaaaaguaa uuuacuuagu aaugaucuua      540

cuaauugcac cagcauacuc aauaagaugc auuggaguau caaauagaga cuuugucgaa      600

gguaugagcg guggaacuug gguagacauu gucuuggagc acgguggaug cguaacugua      660

auggcacaag acaaaccaac aguggacaua gaauugguaa caacaaccgu aaguaauaug      720

gcagaaguua gauccuauug cuacgaggca ucaauaagug acauggcaag ugauucaaga      780

uguccaacac aaggagaggc uuaucucgau aaacaaagug auacccaaua uguauguaaa      840

agaacauuag uugacagagg uuggggaaau ggcuguggac uauucgguaa agggagcuua      900

guaacaugug caaaauuugc auguucaaag aaaaugacag gaaaaucuau acaaccagaa      960

aaucuagaau auaggauuau guuaagcguu cauggaucgc aacauagugg aaugauuguc     1020

aaugacacag gacaugaaac ugacgaaaac cgagcuaaag uggaaauaac accaaauuca     1080

ccacgagcag aagcuacacu cggaggcuuc ggaucacuag gauuggacug ugaaccaaga     1140

acagguuuag auuucagcga ucuauauuau uuaacuauga auaauaaaca uugguuagug     1200

cauaaagaau gguuucauga uauaccauua ccuuggcacg caggagcaga uacaggcaca     1260

ccacacugga auaauaaaga agcccuagua gaauuuaaag augcacacgc uaaaagacaa     1320

acaguaguug uacuaggauc acaagaaggu gcuguacaua cagcacuugc cggggcauua     1380

gaagcagaaa uggauggagc uaaaggaaga uuaagcucag gucacuuaaa auguagauua     1440

aaaauggaua aauuaaggcu aaaaggagua agcuacucuu uauguaccgc agcuuucaca     1500

uuuacaaaaa uaccagcaga aacauuacau ggaacaguaa cuguagaagu acaauaugca     1560

gguacagaug guccauguaa aguaccagca cagauggcag uagauaugca aacauuaaca     1620

ccaguaggga gauugauaac agcuaauccu gucauaacag aaucaacuga aaauagcaaa     1680

augaugcuag aacuagaucc accauucggu gauucauaua uaguaauagg aguuggagaa     1740

aagaaaauaa cgcaucacug gcauagaagc gguaguacaa uaggaaaagc auuugaggca     1800

acaguaaggg gcgcaaaaag aauggcugua uuaggagaua cagcauggga uuucggaagc     1860

gucgguggag cacuaaauuc acuaggaaaa gguauucauc aaauauucgg agcagcuuuu     1920

aaaucuuugu uuggaggaau gaguugguuu ucucaaauac uaauuggaac auugcugaug     1980

ugguuaggac uaaacacuaa aaacggauca auuagcuuaa ugugucuagc ucucggaggu     2040

guauugauau uuuuaucaac agcuguauca gcggacguug gaugc                     2085


<210>  2520
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2520
ggagcugaua caagugucgg aauagucgga cuauuacuaa cuacagcuau ggcagcggaa       60

guuaccagaa gaggcagcgc auauuauaug uauuuagaua gaaaugaugc aggagaagca      120

auauccuuuc cuaccacucu aggaaugaau aaauguuaua uacaaauaau ggaccuagga      180

cacaugugcg augccacuau gaguuacgaa ugcccuaugc uugaugaagg agucgaaccu      240

gaugauguag auugcuggug uaauacaaca aguacauggg uaguauacgg aacaugccau      300

cauaagaaag gagaggccag aagaucaagg agagcaguaa cacuacccuc ucauucaaca      360

agaaaauuac aaacaagaag ccaaacaugg uuggaaagua gagaauauac aaaacaucua      420

auucgaguag aaaauuggau auuuaggaac ccaggauuug cacuagcagc agcagcaauu      480

gcuugguuac uaggaucauc aacaucacaa aaaguaauau accuaguaau gauacuauua      540

auagcaccag cauauaguau aagauguaua ggaguaucua accgagauuu uguugaagga      600

augagcgggg gaacaugggu agauauagug uuagaacaug gaggaugugu uaccguaaug      660

gcacaagaua aacccacagu ggacauagag cuaguuacaa cuaccgucuc aaauauggca      720

gagguacgaa gcuauugcua ugaagcgagc auaagugaua uggcaaguga uagccgaugu      780

ccuacccaag gagaagcaua ucuagauaaa caaucagaca cucaauaugu augcaaacga      840

acauuaguug acagaggaug ggggaaugga uguggacuau uugggaaagg aucucuagua      900

acaugugcaa aauuugcuug uagcaagaaa augacaggua aaucaauaca accagaaaau      960

uuagaguauc gaauuaugcu aagugugcau ggaucacaac auucggguau gauaguaaau     1020

gacacuggac augaaacuga cgaaaauaga gcgaaagucg aaauaacacc aaauucccca     1080

agagcggaag cuacucuagg aggcuucgga agucuggguu uagauuguga accuagaacc     1140

ggacuagauu ucucugauuu auauuauuua acaaugaaua auaaacauug guuaguacau     1200

aaagaguggu uucaugauau uccacuacca uggcaugcug gugcggauac aggaacucca     1260

cauuggaaca auaaagaagc auuaguugaa uuuaaagacg cacaugcuaa acgacaaaca     1320

gucguaguac uaggaaguca agaaggagcu guucacacag cucuagcagg agcauuagaa     1380

gcagaaaugg auggagcaaa aggaagauua ucaaguggac aucuaaaaug uagauuaaaa     1440

auggacaaac uuagauuaaa aggaguaucu uauucucuau guacagcugc cuuuacauuu     1500

acaaaaauac cugcugagac auuacacgga acaguuacag uugaaguuca auaugcaggc     1560

acugauggac cauguaaggu cccagcucaa auggcaguag auaugcaaac auuaacacca     1620

guuggaagau uaauaaccgc aaauccugua auaacagaaa gcacagaaaa uucaaaaaug     1680

auguuagagc uagauccacc auuuggagac ucauacauag uaauaggagu aggugaaaag     1740

aaaauuacac aucauuggca uagaucagga aguacaauag guaaagcauu ugaagcaaca     1800

guaagaggag caaaaagaau ggcaguccuu ggcgauacug caugggauuu uggaucaguu     1860

ggaggagcac uaaacucucu aggcaaggga auacaucaaa uauuuggagc agcuuucaaa     1920

aguuuauuug gaggaauguc augguuuucu caaauacuaa uaggaacauu acuuaugugg     1980

uuaggacuaa auacuaaaaa cggcaguaua ucucuaaugu guuuagcauu aggagguguu     2040

uuaauauuuc uuucuacagc cguaucggca gauguuggau gu                        2082


<210>  2521
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2521
gcugacacau caguuggaau aguaggacua uuauuaacaa ccgcaauggc agcagaagua       60

accagaagag gaucagccua uuauauguau cucgauagga acgacgcagg cgaagcaaua      120

ucauuuccua caacacuagg uaugaauaaa uguuauauuc aaauaaugga ucugggacau      180

augugcgacg caaccauguc cuaugaaugu ccuauguuag augaaggugu agagccagau      240

gauguugacu guugguguaa uacaacuagu acuuggguag uauacggaac augucaucau      300

aaaaaaggag aggcccgaag aucaaggaga gccguaaccu uaccaaguca uucaacaaga      360

aaauuacaaa cuaggaguca gacuuggcua gaaagucgag aauauaccaa acauuuaaua      420

cgaguagaaa auuggauuuu ucgaaaucca ggauuugcac uugcugcugc ugcgauugcc      480

ugguuacuug gaagcucaac aagucaaaaa guaauuuauu uaguuaugau auuauuaaua      540

gcaccagcuu acagcauuag auguauagga gugucaaaua gagacuuugu agagggcaug      600

aguggaggua cguggguaga uauaguauua gaacauggag gaugcgugac aguaauggca      660

caagacaaac caacgguaga uauagaauua guaacaacua ccguaucgaa uauggcagaa      720

guacgaucau auugcuauga agcaucaaua agugauaugg cuuccgacag cagaugcccu      780

acucaaggag aagcauacuu agauaagcaa agugauaccc aauauguuug caaacgcaca      840

cuaguagaua gaggaugggg uaauggaugu gguuuauuug gcaaaggaag ucugguaaca      900

ugugcuaaau uugcaugcuc aaaaaaaaug acaggaaaau caauacaacc cgaaaauuua      960

gaauauagaa uaauguuaag ugugcauggu ucacaacaua gcggaaugau uguaaaugau     1020

acuggacaug aaacagauga aaauagagca aaaguagaaa uaacaccaaa caguccaagg     1080

gcggaagcca cacuaggugg auuugguuca uugggacuag acugugaacc gcgaacuggc     1140

uuagauuuua gcgauuuaua uuauuuaaca augaauaaca aacauugguu aguacacaaa     1200

gaaugguucc augacauucc acuuccaugg cacgcuggcg cagauacugg aacaccucac     1260

uggaauaaca aagaagcacu aguggaauuc aaggacgcac acgcaaaaag acaaacagua     1320

guaguucuag gaucacaaga aggagcagua cauacagcuu uagcgggagc auuagaagca     1380

gaaauggaug gagcaaaagg aagacuauca agcggacauc uaaaauguag guuaaaaaug     1440

gacaaacuaa gauuaaaagg agugucauau aguuuaugua cagcagcauu cacuuuuaca     1500

aaaauuccag cugaaacauu acaugguaca guaacaguug aaguucaaua ugcuggaaca     1560

gauggaccau guaaaguacc agcacaaaug gcaguagaca ugcaaacacu aacacccguu     1620

ggccggcuaa ucacagcaaa uccaguaauu acagaaucaa cugaaaauuc aaaaaugaug     1680

uuagaauuag auccacccuu uggagauagu uacauaguaa uaggcguagg agaaaaaaaa     1740

auuacacauc auuggcauag aucuggguca acaauuggaa aagcguuuga agcaacagua     1800

aggggcgcua aacguauggc aguucuagga gauacggcuu gggauuucgg cucaguagga     1860

ggagcacuaa auucauuagg aaaaggaaua caucaaauuu uuggagcagc auucaaauca     1920

uuauuuggag gaaugagcug guucucacaa auuuuaauag gcacauugcu aauguggcua     1980

ggauuaaaua ccaaaaaugg uucaauaucc uuaauguguu uagcauuagg aggaguuuua     2040

auauuuuuaa guacagcagu aucagcagac guuggaugu                            2079


<210>  2522
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2522
gauacaucag uugguauagu aggacuauug uuaacaacag caauggcggc agaaguaaca       60

agaagaggau cagcguacua cauguaucua gauagaaacg augcagguga agcaauauca      120

uucccaacga cccuaggcau gaacaaaugu uauauacaaa ucauggaucu aggucauaug      180

ugugaugcaa caaugucuua ugaauguccc auguuagaug aaggcguaga gccugaugac      240

guagacuguu gguguaauac aacaaguaca uggguaguuu auggaacaug ucaucauaaa      300

aaaggagaag caagaagaag cagacgagcu gugacacuac cuucacacag uacaagaaaa      360

uuacaaacaa gaucacaaac cugguuggaa agcagagaau auacaaaaca cuuaauacga      420

guagagaacu ggauuuuuag aaauccaggu uucgcauuag cugcagcagc aauagcaugg      480

cuauuaggua guucaacauc ucaaaaagua auauauuugg uaaugauauu acuaauagcu      540

ccagcuuaua guaucagaug uaucggcgua ucaaauagag acuuuguuga agguauguca      600

ggaggaacau gggucgauau aguucuugaa caugguggau guguaacagu aauggcacaa      660

gacaaaccua caguagauau agaauuagua accacaacag uaucaaacau ggcugaagua      720

agaucuuauu guuaugaagc aagcauauca gauauggcaa gcgacagcag augcccaaca      780

caaggagaag cuuaccuaga caaacaaagu gauacucagu acguauguaa acggacacuu      840

guggauagag guuggggaaa cggaugugga uuauuuggaa aaggcaguuu aguaacaugc      900

gcaaaauuug ccuguucaaa aaaaaugacc ggaaaaucaa uacagccaga aaaucuagaa      960

uaccguauaa uguuaagugu acaugguagu caacauagug ggaugaucgu aaaugauaca     1020

ggacaugaaa cagacgaaaa cagagcgaaa guagaaauua caccuaauuc accaagagca     1080

gaagcaacau uagguggauu uggcucacuu ggauuagauu gcgaaccaag aacuggacua     1140

gauuuuucag auuuauacua uuuaacaaug aauaauaaac auugguuagu ucacaaagaa     1200

ugguuucaug auauaccucu accuuggcac gcgggagcag acacaggaac accucacugg     1260

aauaauaaag aagcuuuagu ugaauuuaaa gaugcucacg caaaacgaca aacuguaguu     1320

gucuuagguu cucaagaagg agcaguacau acugcauuag caggcgcucu agaggcagag     1380

auggauggag caaaagguag auuaucgagu ggacauuuga aaugcagauu aaaaauggac     1440

aaauuaaggu uaaaaggagu aaguuacuca uuauguacag cagcauuuac auuuacaaaa     1500

auaccagcag aaacauuaca cggaacugua accguugaag uacaauaugc agguacagau     1560

ggaccaugca aaguaccagc acaaauggcc guagacaugc agacauugac uccaguagga     1620

agauuaauaa cagcaaaucc aguaauaacu gaaagcacgg aaaauaguaa aaugaugcuc     1680

gaauuagauc caccauuugg agauaguuac aucguaaucg gaguuggcga aaaaaaaauu     1740

acacaccacu ggcauagauc ugguucaaca auaggaaaag cauucgaagc aacaguacgc     1800

ggagccaaac gaauggcagu uuuaggagau acagccuggg auuuuggcag uguaggaggc     1860

gcucucaaua gucuuggaaa aggaauacau caaaucuuug gagcagccuu uaagagucug     1920

uucggaggaa ugaguugguu cucacaaaua cuaauaggua cguuauuaau guggcuagga     1980

uuaaacacaa agaaugguuc aauaucuuug augugucuag cuuuaggagg cguacucauc     2040

uuuuugucaa cagcaguauc agcugaugua ggaugc                               2076


<210>  2523
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2523
acaaguguag gaauuguagg auuacuacua acaacagcua uggcagcaga aguaacaaga       60

aggggcucag cauauuauau guauuuagau agaaacgaug cuggggaagc aauaagcuuc      120

ccuacaacau uaggaaugaa caaauguuac auacaaauua uggacuuagg gcauaugugu      180

gaugccacaa ugucuuauga augcccaaug uuagaugaag gaguagagcc ugacgaugua      240

gacuguuggu guaauacaac aucaacuugg gugguauaug gcacauguca ucauaaaaaa      300

ggcgaagcac gaagauccag gagagcugua acacuaccau cacauaguac aagaaaauua      360

caaacaaggu cucaaacaug guuagaaucu cgcgaauaua ccaaacaccu uauaagagua      420

gaaaauugga uauucagaaa uccaggcuuc gcacuagcag cugcagcuau agcaugguua      480

uuaggaucgu ccacaucaca aaaagucaua uauuuaguaa ugauacuauu aaucgcacca      540

gcauauucaa uaagguguau aggaguguca aacagagauu uuguugaagg aauguccgga      600

ggaacauggg uggacauagu ucuugaacac ggaggaugug uuacaguuau ggcucaagau      660

aaaccaaccg uugacauaga auuaguaaca acaacuguaa guaauauggc agaaguaaga      720

aguuacuguu augaagcaag cauaucagau auggcaucag acucaagaug cccaacacaa      780

ggugaagcau auuuggauaa acaaucagau acacaauaug uauguaaaag aacacuagua      840

gauagagguu gggggaacgg auguggauua uuuggaaaag gcagcuuagu aacaugugca      900

aaauuugcau gcagcaaaaa aaugacagga aaaucuauac aaccagaaaa ucuagaauac      960

agaauaaugu ugucaguaca ugggucacaa cauucaggaa ugauaguaaa cgauaccggg     1020

cacgaaacag acgagaaccg agcgaaaguu gaaauaacac caaacagccc aagagcugaa     1080

gcaacauuag ggggauucgg cucauuagga cuagauugcg agccaagaac aggauuagac     1140

uuuucagauc uguauuauuu aacaaugaac aauaaacauu gguuaguuca uaaagaaugg     1200

uuucacgaua uuccauuacc auggcaugcg ggagcagaua ccggaacacc acauuggaau     1260

aacaaagaag cuuuaguaga auucaaagac gcacaugcaa aaagacaaac aguuguagua     1320

uuagguagcc aggaaggggc cguacauaca gcacuugcag gagcauuaga ggcagaaaug     1380

gauggugcua aaggcagacu aaguagugga caucuaaaau guaggcuaaa aauggauaaa     1440

uugagacuca aaggagucuc auauagucua uguaccgcag cauucacauu uacaaaaaua     1500

ccugcagaaa cacuucaugg aacaguaacu guugaaguac aauacgcagg gacugaugga     1560

ccauguaaag ucccagcaca aauggcagua gauaugcaaa cauuaacacc uguugguagg     1620

uuaauaacag caaacccugu gauaacugaa ucuacagaaa acagcaaaau gauguuagag     1680

uuagacccac cauuuggaga uaguuauaua gucauaggag uaggcgaaaa aaaaauaacu     1740

caucauuggc acagaucagg caguacgaua gguaaagcau uugaggcaac aguacgcgga     1800

gcgaaacgaa uggcaguacu aggagauaca gcaugggauu ucggcucagu uggaggagca     1860

uuaaauucac uuggaaaagg cauacaccaa auauuuggug cagcauucaa aagccuauuu     1920

ggaggaaugu caugguuuag ucaaauauua auaggaacau ugcuaaugug gcuuggauua     1980

aauacaaaaa auggauccau aucacuaaug ugccuagcau uaggaggagu auuaauauuc     2040

cuaucuacgg cagugagcgc cgauguagga ugc                                  2073


<210>  2524
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2524
acuagcguag gaauagucgg acuucuauug acaaccgcua uggcagcagc aguuacaaga       60

agaggcuccg cauauuacau guaccuagac agaaaugaug ccggagaagc cauaagcuuu      120

ccaacaacac uaggaaugaa uaaauguuac auucaaauca uggaucucgg acauaugugu      180

gaugcaacaa ugaguuauga auguccaaug uuagacgaag gaguagaacc ugaugauguc      240

gauugcuggu gcaauacaac aucaacaugg gucguauaug gcacuugcca ccauaaaaag      300

ggagaagcua ggcguucacg aagagcagua acacuaccau cccauucaac uagaaaauua      360

caaacccgaa gucaaacuug guuagaaucc agagaauaua caaaacacuu gauaagagua      420

gaaaauugga uauuuagaaa uccagguuuc gccuuagcag cugcagcaau ugcaugguua      480

cuaggaagcu caacaucaca aaaaguuaua uacuuaguaa ugauacuauu aauagcucca      540

gcuuacucaa uaagauguau aggaguuuca aauagggauu ucguagaagg gaugucagga      600

gguacauggg ucgacauagu auuagaacau ggaggaugcg uaacaguuau ggcucaagau      660

aaaccaacgg ucgauaucga acuuguuaca acuacaguau cuaauauggc agaaguaaga      720

aguuauuguu augaggcauc uauaagugau auggcuagug auaguagaug uccaacacaa      780

ggugaagcau acuuagauaa acaaucugac acacaauaug uuuguaaaag aacacuagua      840

gaccgaggau ggggcaacgg guguggauua uuuggaaaag gaaguuuagu aacaugcgca      900

aaauuugcau guucaaaaaa aaugacagga aaaucgauuc aaccugaaaa ucuagaauau      960

agaaucaugc uuucaguuca cggaucacag cauaguggaa ugauaguaaa ugauacaggu     1020

cacgaaacgg acgaaaauag agccaaagua gaaauaacuc cgaauucacc aagagcggaa     1080

gccacucuug gcggauuugg aucauuagga uuagauugug aaccaaggac aggacuagau     1140

uucucagauc uauauuaccu uacaaugaau aacaaacauu gguuaguaca caaagaaugg     1200

uuucaugaua uaccacuacc uuggcacgca ggagcugaua cugguacacc acacuggaac     1260

aacaaagaag cguuaguaga auuuaaagau gcacaugcaa aaagacaaac cguaguagua     1320

cucggaagcc aagaaggagc aguacauaca gcucuagcug gagcauuaga agcagaaaug     1380

gacggagcaa aaggaagauu aucuaguggc caccuuaaau guagauuaaa aauggauaag     1440

uuaagacuca aagguguauc auacucauua uguacugcug cauuuacauu cacgaaaaua     1500

ccugcagaaa cacuacaugg aacuguaacu guugaagucc aauaugcagg uacagaugga     1560

ccauguaaag uaccagcaca aauggcuguc gauaugcaaa cguuaacucc uguaggacga     1620

uuaauaacag caaauccagu uauaacggaa uccacagaaa acucaaaaau gauguuagaa     1680

uuagauccac cauuuggaga uucauauaua guuaucggug uuggagaaaa aaaaauaacu     1740

caucauuggc auagaagugg uucaaccaua ggaaaagcau uugaggcaac uguaagagga     1800

gcaaaaagaa uggcugugcu gggagauaca gcuugggauu uuggaagugu gggcggagca     1860

uuaaauagcu uagguaaagg uauacaucaa auauuuggag cagcauuuaa aucacuauuu     1920

gguggaaugu caugguuuag ucaaauacug auagguacuc uuuugaugug guuaggauua     1980

aauacaaaaa auggaagcau aaguuuaaug uguuuagcau uaggaggagu auuaauauuu     2040

cuaagcacag caguaucagc ugacguagga ugu                                  2073


<210>  2525
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2525
agaggagcag auaccagugu aggaauagua ggucuauuau uaacaacagc uauggcagca       60

gccguaacca gaagaggaag ugcauauuau auguaccuug acaggaauga ugcgggugaa      120

gcaauuaguu uuccaacaac acuugggaug aauaaauguu auauucaaau uauggaucuu      180

ggucauaugu gugaugcaac aaugagcuac gaauguccua ugcucgauga aggaguagaa      240

ccagaugaug uagauuguug guguaauaca acgagcacuu ggguaguaua uggaaccugc      300

caucauaaga aaggggaagc uagaagaagu aggagagcag uaacacugcc aucacauucg      360

acaagaaaac uacagacaag aagucaaaca ugguuggaaa guagagaaua cacaaaacau      420

uuaauuagag uagaaaacug gauauuuaga aauccuggau ucgcauuagc agcagccgcc      480

auagcuuggu uguuaggaag uagcacaagu caaaaaguga uauauuuagu uaugauauua      540

cuaauagcuc cugcauauag uauaagaugc auaggaguaa guaauagaga cuuuguugaa      600

gguauguccg ggggaacaug gguagacaua guacuagaac auggaggaug uguuacaguu      660

auggcacaag auaaaccuac aguggauauu gaacuuguaa cuacaacggu cucaaauaug      720

gcagaaguac gaucauauug uuacgaagca aguauaucug auauggcauc ugacucaaga      780

ugcccaacgc aaggagaagc auaccuugau aaacaaagug auacacaaua cguaugcaaa      840

agaacucuag uagacagagg auggggaaau ggcuguggau uauucggcaa aggaucucuu      900

guuacaugcg caaaauuugc cugcucgaag aaaaugacag gaaaaaguau ucaaccagaa      960

aauuuagaau auagaauaau guuaagugug cacgguucac aacauuccgg uaugaucgua     1020

aacgauacag gacaugaaac agaugaaaau agagcuaaag uagagauuac accaaauagu     1080

ccacgagccg aagcaacacu cggcggauuu ggcaguuuag guuuagauug cgaaccgaga     1140

acagguuuag auuuuucaga uuuauauuau uuaaccauga acaauaaaca uugguuaguc     1200

cauaaagaau gguuucacga cauaccucua ccauggcaug caggagcaga uacagguacc     1260

ccucauugga auaauaaaga ggcuuuagua gaauuuaaag augcacacgc aaaaagacaa     1320

acagugguug uauuagguag ccaagaaggu gcaguacaua cagcucuagc aggagcauua     1380

gaagcggaaa uggauggugc aaaaggaaga uuaaguucag ggcacuuaaa auguagauua     1440

aagauggaca aguuacgauu aaaaggagua ucauauagcc uauguacagc ggcauuuaca     1500

uuuacuaaaa uaccagcaga aacacuacac ggaacaguaa caguugaagu acaauaugcu     1560

ggaacagaug gccccuguaa aguaccagca caaauggcag uagacaugca aacacuaacc     1620

ccaguaggaa gauuaauaac agcaaauccg guaauuacug aaucuacuga aaauagcaaa     1680

augaugcuag aauuagaucc accauucggc gacagcuaua uagucauagg ggugggagaa     1740

aagaaaauua cacaucauug gcacagauca ggaaguacua ucgguaaagc auuugaagca     1800

acaguaagag gagcuaaaag aauggcagua cuaggggaua cagcauggga cuuuggaagu     1860

gucgguggag cacuaaauuc uuugggaaaa gggauacacc aaauauuugg ugcagcauuu     1920

aaaaguuuau ucgguggaau gucuugguuc agucagauac uaauuggcac ucuuuuaaug     1980

ugguuagguc uaaauacgaa aaauggguca auaucucuaa ugugucuagc auuaggaggu     2040

guuuuaaucu ucuuaagcac agcaguauca gcagacguag gaugu                     2085


<210>  2526
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2526
cgaggacugu caucaagaaa aagaagauca caugauguac uuacaguaca auucuuaaua       60

uuaggaaugu uacuaaugac aggaggcgua acaagaagag guagcgccua cuauauguau      120

cuagauagaa augaugcagg agaagcaauc aguuucccua caacccuugg uaugaauaaa      180

ugcuauauac aaauaaugga uuuggggcac augugugacg cuacaaugag uuacgaaugu      240

ccaauguuag augaaggagu cgaaccagau gauguagauu gcugguguaa caccacauca      300

acaugggugg uauacgggac augccaucau aagaaaggag aagcaagacg uucaagacga      360

gcuguaacau uaccaaguca uagcacaaga aaauuacaaa cuagaucaca aacaugguua      420

gaaucccgag aguacacaaa acaucuaaua agaguggaaa auuggauuuu uagaaaucca      480

gguuucgcau uagcugcagc cgcuauagcc ugguuauuag guucaucuac aagucaaaaa      540

guaauauauu uaguaaugau auuacuaaua gcaccagcau auucuauaag auguauagga      600

gucucaaaua gagauuucgu agaagguaug agcggaggaa cuuggguaga uauuguacua      660

gaacauggug gcuguguaac aguaauggcu caagauaaac cuacaguaga uauagaauua      720

guaacaacga caguaucaaa uauggcagaa guuagaucuu auuguuacga agcaagcauc      780

ucagauaugg caagugacag uagaugccca acacaagggg aagcauaucu agacaaacaa      840

ucagacacuc aauauguaug uaaaagaacu uuaguugacc gaggaugggg aaaugguugu      900

ggacuauucg guaaaggauc auuaguuacu ugugcgaaau ucgccugcag caagaaaaug      960

acaggaaaau caauccaacc agaaaacuua gaauacagaa uaaugcuaag uguacaugga     1020

agccagcaca guggaaugau aguaaaugau acaggccaug aaacagauga aaauagagca     1080

aaaguagaaa uaacaccaaa uucaccgaga gcugaggcaa cacuaggagg auuugggagc     1140

uuaggauuag auugugaacc aagaacaggc cuagacuuua gcgauuuaua uuacuuaaca     1200

augaauaaua aacacuggcu aguacacaaa gaaugguuuc augacauacc uuuaccaugg     1260

caugcaggag cagacacugg uacaccacau uggaacaaua aagaagcauu aguagaauuu     1320

aaagacgccc augcgaaaag acaaacggua guaguacuag gaagucaaga aggggcagua     1380

cauacagcuu uagcuggugc auuagaagcu gaaauggaug gagcaaaagg uagacuaucc     1440

ucaggucacu uaaaauguag acuaaaaaug gacaaauuaa gguugaaagg aguaucauac     1500

aguuuaugua cagcagcauu uacauuuacu aaaauaccug cagagacacu gcauggaacc     1560

guuacagugg aaguacaaua cgcaggaaca gauggaccau guaaaguacc agcacaaaug     1620

gcgguagaua ugcaaacacu aaccccggua gguagauuaa uaacagcaaa cccugugaua     1680

acagaaagca cugaaaacuc aaagaugaug uuagaauuag acccaccauu cggugauagu     1740

uauauaguaa uaggagucgg agaaaagaaa auaacacauc acuggcauag aagugguuca     1800

acaauaggua aagcguucga agcaacaguu cgaggagcaa aaagaauggc cguacuagga     1860

gauacugcau gggacuuugg aucaguagga ggagcuuuaa acucucuagg uaaagguauu     1920

caucaaauau ucggggcugc uuuuaagagc uuauuuggcg gaaugucuug guuuucacaa     1980

auauuaauag gaacacuauu aauguggcua ggacuaaaua cgaaaaaugg aagcauaagu     2040

cugauguguu uagcacuggg aggaguacuc aucuuccuaa gcacagcagu uucagccgau     2100

guaggaugu                                                             2109


<210>  2527
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2527
cgaggacuau caucaagaaa aagaagauca caugacgucc uuacaguaca auuuuuaaua       60

uuaggaaugu uauuaaugac cggagcagua acuagacgag gaucagcaua uuacauguac      120

cuagaccgaa augacgcagg agaagcuaua ucauuuccua caacccuagg aaugaacaaa      180

uguuacauac aaauaaugga uuuaggacau augugugaug ccacaauguc auaugaaugu      240

ccaaugcucg augaaggagu agaaccggau gacgucgauu guuggugcaa cacaacuucg      300

acuuggguag uauacgguac uugucaucau aagaaaggag aagcgagacg uucaaggaga      360

gcagugacac uaccgucuca cucaacaaga aaauuacaaa caagaucaca aacgugguua      420

gaaagcagag aauauacaaa acacuuaauc agaguggaaa auuggauauu uaggaaccca      480

ggcuucgcuu uagcagcggc agcaauagca ugguuacuag gauccagcac aucacaaaaa      540

guaauauacu ugguaaugau auuacuaauu gcuccagcau acucaauuag auguauaggu      600

guaaguaaca gagauuucgu ugaaggaaug aguggaggaa caugggucga uauaguacuu      660

gagcauggcg gaugcguaac cguaauggca caagauaagc caacuguaga uauagaacug      720

guuacaacaa cagucucuaa uauggcagaa guaagaagcu auuguuauga agcgucaaua      780

ucagauaugg cgucagacuc aagaugcccc acccaaggag aagcauaucu agauaaacaa      840

agugacacac aauauguaug caaaaggacu cuaguagaua gaggaugggg aaaugggugu      900

ggauuauucg gaaaaggguc ccuaguaaca ugcgcaaaau uugccugcuc aaagaaaaug      960

acagggaaau caauucaacc agaaaaccua gaauacagaa uaauguuaag cguucaugga     1020

agucaacauu cggguaugau uguaaacgau acaggacaug aaacugacga aaauagagcg     1080

aaaguugaaa uaacaccuaa uucaccaaga gcagaagcaa cucucggagg auucggaagu     1140

cuuggacugg auugcgaacc acgaacaggg uuagauuuua gcgaccuaua uuaucuaacg     1200

augaauaaca aacauugguu aguacacaaa gaaugguuuc augacauccc acuaccaugg     1260

caugcaggag cagacacagg uacgccacau uggaauaaca aagaagccuu aguagaauuu     1320

aaggaugcac augcaaaaag gcaaacugua guugucuuag gaagccaaga aggcgcagua     1380

cauacagcau uagcuggagc uuuagaggcu gaaauggaug gagcaaaagg uagguuauca     1440

aguggacauu uaaaaugucg auuaaaaaug gauaaauuaa gguuaaaagg aguaucuuau     1500

ucauuaugca cagcagcauu uacauuuaca aaaauaccag cagaaacccu acaugguaca     1560

guaacaguag aaguccaaua ugcaggcaca gauggaccau gcaaaguucc agcucaaaug     1620

gccguagaua ugcaaacuuu aacacccguu ggaagacuaa uaacagcaaa uccaguaaua     1680

acagaaucaa cagaaaauag uaaaaugaug cuagaacuag aucccccauu uggcgauagc     1740

uauauaguca uaggggucgg agaaaagaaa auuacacauc acuggcaucg aucaggcagu     1800

acaauaggua aagcauuuga agcaacagua cgaggagcua aaagaauggc aguccuagga     1860

gauacagcuu gggacuuugg uucaguagga ggagcauuaa acucauuagg aaaagguaua     1920

caucaaauau uuggagcagc uuucaaauca uuauuuggag gcaugucuug guuuagucag     1980

auacuaauag gaacucuauu aaugugguua ggcuuaaaua cuaaaaaugg aagcauaucu     2040

uuaauguguc uagcuuuagg uggaguauua auauuuuuaa gcacagcugu aucagcugau     2100

guaggcugu                                                             2109


<210>  2528
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2528
aggauaauaa augcccgcaa agaaaagaaa cggagaggag ccgauacauc uguagguauu       60

guaggauuau uauuaacaac agcgauggca gcagaaguaa caagaagagg aucagcuuau      120

uauauguauu uagacagaaa cgaugcagga gaagcaauua gcuuuccaac uacauuaggu      180

augaauaaau guuauauaca aauaauggau uuaggacaua ugugugaugc gacaaugucc      240

uaugaauguc caaugcuaga ugaaggugua gagccagaug auguagauug cugguguaau      300

acaacaucaa caugggucgu auauggaaca ugccaucaua agaaagguga agcaaggcgu      360

aguaggagag caguuacauu accaucucau ucuacuagaa aacuacaaac aagaucacaa      420

acaugguuag aaagucgaga auauacuaaa cauuuaauaa gaguagaaaa cuggauuuuu      480

agaaacccag gauuugcacu agcugcagcu gcaauagcau gguuguuggg aagcaguaca      540

agucagaaag uaauuuauuu aguaaugaua uuauuaauug caccagcaua uucaauuaga      600

uguauaggug uaucuaacag agacuuugua gagggcaugu caggaggaac auggguugau      660

auuguuuuag aacauggugg auguguaacu guaauggcuc aagauaaacc aacaguagac      720

aucgaauuag ugacuacaac aguaucaaac auggcugagg uuagauccua uuguuaugaa      780

gccucaauca gcgauauggc cagugauagu agauguccaa cacaagguga agcauauuua      840

gauaaacaaa gugauacgca auauguuugu aaaaggacgu uaguagaucg gggcugggga      900

aacggaugcg gcuuauuugg aaaaggaagu uuaguaacau gugcaaaauu ugcaugcuca      960

aagaaaauga cagguaaaag cauucagccu gaaaauuuag aauauagaau aaugcuaagu     1020

guacauggca gucaacauuc aggaaugauu guaaaugaca cgggacacga aacagacgaa     1080

aacagagcaa aagucgaaau aacgccuaau aguccaagag cagaagcaac uuuaggugga     1140

uuuggaagcc uuggacuaga uugugaacca cgaacugguu uggauuucag ugauuuauac     1200

uauuuaacua ugaauaacaa acacugguua guacauaaag aaugguuuca ugacauccca     1260

uuaccauggc augcaggagc ggauacuggg acaccacauu ggaacaauaa agaggcguua     1320

gucgaguuca aagaugcaca ugcgaaacga cagacaguag uaguauuggg aagucaagaa     1380

ggagccguac acacagcauu agcuggagca uuagaagcug aaauggacgg agcaaaagga     1440

agauuaucau cuggacacuu gaaauguaga uuaaaaaugg auaaacucag acugaaagga     1500

guaagcuaca gucuauguac agcagcauuc acauuuacaa aaauuccugc agaaacacua     1560

cauggaacag uuacaguaga aguacaauau gcaggaacag auggaccaug uaaaguaccg     1620

gcacaaaugg cuguagacau gcaaacacuu acgccuguag gaagguuaau uacagcaaac     1680

ccugugauaa cagaaaguac ggaaaauuca aaaaugaugu uagaacuaga cccuccauuc     1740

ggugauaguu auauagucau aggaguaggu gaaaagaaaa uuacacacca uuggcaucgc     1800

ucaggcucaa caauaggaaa agcguuugaa gcgacaguaa ggggagcaaa aagaauggca     1860

guguuagggg auacagcaug ggauuucgga ucgguaggag gugcauugaa uagucuagga     1920

aaagguauuc accaaauauu uggagcagcg uucaaaucau uauuuggggg uaugaguugg     1980

uucucacaaa uauuaauagg gaccuuauua auguggcuag gacuaaauac gaaaaauggu     2040

ucaauuaguc ucauguguuu agcauuaggu ggaguauuga uauuccuaag uacagcagua     2100

uccgcagau                                                             2109


<210>  2529
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2529
auaauuaacg caagaaaaga aaaaaagaga aggggggcag acacaucugu aggaaucgua       60

ggguuauuau uaacaacagc uauggcagcc gaaguaacgc gaagaggauc agcuuauuau      120

auguauuuag acagaaacga ugcaggagaa gcaauaaguu uuccaacuac ucuaggaaug      180

aauaaauguu acauacaaau aauggauuua ggacacaugu gugacgcaac aaugagcuau      240

gaaugcccaa uguuggauga aggcguagag cccgaugaug uggauuguug guguaauaca      300

acaagcacuu gggugguaua uggcacuugc caucacaaaa aaggcgaagc aagaaggucu      360

agaagagcag uaacauuacc aucccacuca acaagaaaau uacaaacaag aucccaaaca      420

uggcuagaau cuagagaaua uacaaaacau uuaauucgcg uagaaaauug gauauucaga      480

aauccggggu uugcacuagc agcagcugca auagcauggc uauuaggauc aucgacaagc      540

caaaaaguaa uauauuuagu aaugauacua cucauagcac cugcauacuc aauaagaugu      600

aucgguguau caaauagaga uuucguggaa ggcaugagug guggaacaug gguugacaua      660

guuuuagaac auggagguug uguuacugua auggcucaag auaaaccaac aguagauaua      720

gaacucguaa caacaacagu uucaaauaug gcggaaguaa gaaguuauug uuaugaagcu      780

ucuauuucag auauggccag cgacagucgu ugcccaacac aaggugaagc auaucuagac      840

aagcaaagug auacucaaua uguaugcaaa agaacacuag uagacagagg uugggguaau      900

ggaugugguu uauuuggaaa aggaagccua guaacaugcg caaaauuugc uugcaguaaa      960

aaaaugacag ggaaaaguau acagcccgaa aaccucgagu auagaauaau guuaucugua     1020

cauggaucac aacacagcgg aaugauagua aaugauacag gacacgaaac agaugaaaac     1080

agagcaaaag ucgaaauaac accaaauagu ccucgagcug aagcuacauu aggaggauuc     1140

ggaucgcuag gguuagauug cgaaccuaga acuggauuag acuuuucuga uuuauauuac     1200

cugacaauga acaacaaaca cuggcuugua cauaaagaau gguuccauga uauuccauua     1260

ccauggcaug ccggagcaga uacaggaacc ccccauugga auaauaaaga agcauuagua     1320

gaauucaaag acgcgcacgc uaaaagacaa acaguaguag uacuaggauc acaagaagga     1380

gcuguacaua cagcauuggc aggagccuua gaagccgaaa uggacggagc aaaaggucgu     1440

cuuucaagcg gacauuugaa auguagacua aaaauggaua aacucagacu aaaaggggua     1500

ucauauucac uauguacagc agcauuuaca uuuacaaaaa uaccagcaga aacauuacau     1560

gguacaguca caguagaagu ucaauaugca ggaacagaug guccguguaa aguaccugca     1620

caaauggcag uagauaugca gacauuaaca ccgguuggua gguuaauuac cgcaaaucca     1680

guaauaacag aaaguacaga aaauucaaaa augauguuag aauuagaucc accauuugga     1740

gacagcuaua uaguaauagg aguaggugaa aaaaaaauaa cacaucauug gcauagaagu     1800

ggaucaacaa uaggaaaagc auuugaagca acggucagag gagcaaaaag aauggccguu     1860

uuaggggaua cagcauggga uuuuggaagu guagguggag cauuaaacuc uuuagguaaa     1920

ggcauacauc aaauauuugg agcggcuuuu aaaaguuuau ucggugguau gucaugguuu     1980

agucaaauac uaauaggaac uuuauuaaug ugguugggau uaaauacaaa aaacgguucg     2040

auuaguuuaa ugugcuuagc auuaggcgga guauuaauau ucuuaaguac ggcgguaagu     2100

gcugau                                                                2106


<210>  2530
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2530
auaaaugcaa ggaaagaaaa aaaaagaaga ggagcagaua cauccguugg aauaguugga       60

cugcuauuaa caacagcuau ggcagcagag guuacacgaa gaggaagcgc auauuauaug      120

uaucuagaua gaaaugaugc aggagaagcu auaaguuuuc cgacaacauu aggaaugaau      180

aaauguuaua uacaaauaau ggaccuagga cauaugugug augcaacaau gaguuaugaa      240

uguccaaugu uggacgaagg aguagaaccc gacgacguag auuguuggug uaauacaaca      300

agcaccuggg uaguauaugg cacaugucac cauaagaaag gagaagcaag aagaucacga      360

agagcgguaa cauuaccauc acauucuaca cgaaaauuac aaacaagguc acaaacaugg      420

uuagaaagca gagaauauac aaaacauuua auucgagugg aaaauuggau auuuagaaau      480

ccaggauuug cacuagccgc agcagcaaua gcaugguuac uaggaucauc aacaucacaa      540

aaaguaauau auuuaguaau gauacuacuu auagcaccag ccuacucaau uagaugcauu      600

ggaguaucua auagagauuu cguagaagga augagcggag gaacuugggu ggauauugua      660

uuagaacacg gaggaugugu aacaguaaug gcucaagaua agccaacagu agacauagaa      720

uuaguaacaa cuacaguaag uaauauggcc gaggucagaa gcuacugcua ugaagcauca      780

auuucagaua uggcaagcga uucaagaugu ccaacacaag gugaagcuua uuuggacaaa      840

caaagugaca cacaauaugu augcaaacga acacugguag aucgcggaug gggaaaugga      900

uguggauuau uuggaaaagg gucauuagua acaugugcaa aauucgccug uucaaaaaaa      960

augacuggaa aauccauuca acccgaaaau cuagaauaua gaauaauguu aaguguacau     1020

ggaucacaac auucugguau gauaguaaau gauacagguc augaaacaga ugaaaaccgc     1080

gcaaaaguag aaauaacacc uaacucaccu agagcagaag caacacuagg aggauuugga     1140

agcuuagggu uggacuguga accuagaaca ggacuggacu uuucugacuu auauuaucua     1200

acaaugaaca auaaacauug guuaguacau aaagaauggu uccaugauau accauuacca     1260

uggcaugcag gagcugauac aggaacaccu cacuggaaca auaaagaagc acucguagaa     1320

uuuaaagaug cccaugcgaa aagacaaacu guaguaguuu uaggauccca agaaggcgcu     1380

guacacacug cacuagcugg ggcauuagaa gccgaaaugg acggugcaaa gggacgauua     1440

ucauccggac auuugaaaug ucguuuaaaa auggacaaau uaagacuaaa aggaguaucg     1500

uauucacuau guacagcagc cuuuacuuuu acaaaaauac cagcagaaac auuacaugga     1560

acaguuacag uugaaguaca auaugccgga acagacggac caugcaaagu uccagcacaa     1620

auggcuguag auaugcaaac auuaacucca guaggcagau ugauaacagc aaacccuguc     1680

auaacagaau caacugaaaa caguaaaaug augcuugaac uagacccccc auuuggagac     1740

ucauacauag uaauaggggu aggugaaaaa aaaauaacac accauuggca uagaagugga     1800

uccacaauag guaaagccuu ugaagcaacu guacguggag caaaaagaau ggcagucuua     1860

ggggauacag caugggauuu uggaucagua ggaggagcuu uaaacucuuu aggaaaagga     1920

auacaucaaa uauuuggagc agcauucaaa ucacuuuuug gaggaauguc augguuuucu     1980

caaauucuaa ucgggacacu acuuaugugg cuaggauuaa auaccaaaaa cggcaguaua     2040

ucacugaugu guuuggcauu agguggaguu uuaauauuuu ugagcacagc ggugucagca     2100

gau                                                                   2103


<210>  2531
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2531
aaugcgagga aagaaaagaa aagaagaggu gcagauacau cagucggaau aguaggacug       60

uuauugacaa cagcgauggc agcagaagua acaagaagag gaagcgcaua uuauauguac      120

uuagauagaa acgaugcugg ugaagcaaua ucauuuccua caacauuagg aaugaacaaa      180

uguuauauac aaauaaugga uuugggacau augugugaug cuacaaugag uuaugaaugu      240

ccaauguugg augaaggagu agagccagac gauguagauu guugguguaa cacaacauca      300

acauggguag uauaugggac uugucaccau aagaaagggg aagcucgacg aagccguaga      360

gcgguaacgc uaccaagcca uagcacaaga aaauuacaaa cgagaaguca aacuugguua      420

gaaagcagag aauacacaaa acauuuaaua agaguagaaa auuggauauu cagaaaccca      480

ggguuugcac uagcggcggc ugcaauagca uggcuccuag gaucaucaac aucacagaaa      540

guaauauacc uaguaaugau acuacuaaua gcuccugcau auaguaucag auguauagga      600

guaaguaauc gagauuuugu agaagguaug uccgguggaa cguggguaga cauaguacua      660

gaacauggag gauguguaac agucauggca caagacaaac cgacaguaga cauagaauua      720

guuacgacaa caguaucaaa uauggcagaa gugagaucuu auuguuacga agcaagcaua      780

agcgacaugg caagugacag ccguugucca acacaaggag aagcauacuu agauaaacaa      840

agugauacac aauauguaug uaaaagaacg cuaguugaca ggggcugggg aaacggaugc      900

ggcuuauucg gaaaaggauc ucuaguuaca ugugcaaaau uugcauguuc aaagaaaaug      960

acaggcaaau caauacaacc ggaaaauuua gaauauagga uaauguuauc agugcauggu     1020

ucacaacauu caggaaugau aguuaacgau accggucaug agacagacga aaauagagca     1080

aaaguagaaa uaacacccaa cucaccaaga gcagaagcaa cacuaggugg auucgguagc     1140

uuaggauuag acugugaacc aaggacagga uuggauuuua gugaccuaua uuaccuaaca     1200

augaauaaua aacacuggcu aguacauaaa gaaugguuuc augauauacc guuaccuugg     1260

caugcuggug cugauacagg gacuccacau uggaauaaua aagaagcauu aguagaauuu     1320

aaagaugcgc acgcgaaaag acaaacggua guaguauuag ggucacagga aggagcagua     1380

cauacugcac uagcaggagc auuagaagca gaaauggaug gagcuaaagg aagacucagc     1440

ucagggcacu uaaaaugcag auugaaaaug gauaaauuaa gacuuaaagg uguuaguuau     1500

ucucucugua cugcagcauu uacuuucacu aaaauaccgg cagaaacacu ucauggcaca     1560

guaacagucg aaguacagua cgcaggaaca gaugggccau guaaaguacc agcacaaaug     1620

gcuguagaua ugcaaacguu aacaccugua ggacgauuaa uaaccgcaaa ccccguaauu     1680

acagaaucaa cagaaaacuc aaaaaugaug cuugaauuag acccaccauu cggagauagc     1740

uauauaguaa uaggaguggg agaaaagaaa auaacacauc auuggcauag aagcgguucu     1800

acaauaggua aagcauucga ggcaacgguu agaggggcaa aaagaauggc gguauuagga     1860

gacacagcuu gggauuucgg aagcguaggu ggagcauuaa auucacuugg aaaagguaua     1920

caucaaauau uuggagcagc auucaaaagu uuauucggag guaugucaug guuuagucaa     1980

auuuugauag gaacauuacu gaugugguua ggguuaaaua cuaaaaacgg aagcauuagc     2040

uuaauguguc uugcucuagg aggcguauua auauucuuaa gcacagcagu uagugcagac     2100


<210>  2532
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2532
gcuagaaaag aaaagaaacg aagaggcgca gauacaucag uaggaauagu uggucuauua       60

cuaacaacug caauggcagc agaaguaacc agaaggggaa gugccuauua uauguaucuu      120

gauaggaaug augcugguga ggcaauauca uuuccuacaa cucuuggaau gaauaaaugc      180

uacauacaaa uaauggauuu aggucauaug ugcgaugcca caaugucaua cgaaugccca      240

augcuagaug aaggaguaga accugaugau guagacuguu ggugcaacac aaccaguaca      300

uggguaguau auggaacuug ucaucacaag aaaggagaag caagaagaag uagacgagca      360

guaacgcuac caagccacuc aacacggaaa uuacaaacaa gaagccaaac augguuagaa      420

agcagagagu auacgaaaca ccucaucaga guagaaaacu ggauauuuag aaacccggga      480

uuugcauuag cagcagcggc aauagcaugg cuauuaggaa guuccacaag ucaaaaaguu      540

aucuacuuag uaaugauacu uuugauagca ccagcauacu caauuagaug uaucgggguu      600

ucuaauagag auuuuguaga aggaaugagu ggagguacau ggguagauau aguauuagaa      660

cacggaggau gugucacagu aauggcacaa gauaaaccaa caguugacau agaauuagug      720

acaacaacag uaagcaauau ggcagaagua cgauccuacu guuacgaagc aaguauaagc      780

gauauggcua gugacucacg auguccuacu caaggugagg cuuaccuaga uaaacaaagc      840

gauacgcagu auguaugcaa aagaacauua guagacaggg gaugggguaa cggaugcggg      900

cuauuuggaa aaggcucucu uguaacaugu gcaaaauucg cuuguaguaa gaaaaugacg      960

gguaaaucaa uacaaccaga aaaucuagaa uauagaauaa uguuaagugu acauggaucu     1020

caacauagug ggaugaucgu aaaugauaca ggucacgaaa cagacgaaaa cagagcuaaa     1080

guagaaauaa caccaaauag cccgagagca gaagcuaccu ugggaggauu uggaagccua     1140

ggucuugauu gugaacccag aacaggauua gauuucucag auuuauauua ccuaaccaug     1200

aauaauaaac auuggcuggu acauaaagaa ugguuucaug auauaccuuu accauggcac     1260

gcgggggcag acaccgguac uccacacugg aauaauaaag aggcauuagu agaauuuaaa     1320

gacgcacaug caaaaaggca aacaguagug guacuaggua gccaagaagg agcaguacau     1380

accgcauuag caggagcacu agaggcugaa auggauggag caaaaggaag acuaucauca     1440

ggacaucuua aaugucgauu aaaaauggac aaauuaagau uaaaaggggu aagcuauagu     1500

uuaugcacgg cagcuuuuac auuuaccaaa auaccagcag aaaccuuaca ugguaccgua     1560

accguugaag uucaauaugc uggaacagac ggaccaugua aaguacccgc acaaauggca     1620

guagauaugc aaacacuaac accaguuggg cgacugauaa cugcaaaucc aguaauaaca     1680

gagucaacag aaaacucaaa gaugauguua gaauuagauc caccauuugg ugauucauau     1740

auaguaauag gaguuggaga aaagaaaauc acacaucauu ggcauagaag cggaaguacu     1800

auaggaaagg cuuuugaagc aacaguaaga ggagcaaaac gaauggcagu cuuaggagau     1860

acugcuuggg auuucggaag uguaggagga gcuuuaaaca gccucgguaa agggauacau     1920

caaauauuug gagcagcauu uaaaaguuua uuuggaggaa ugaguugguu uucacaaaua     1980

uuaauuggca cauuacuuau guggcuaggu uuaaauacga aaaacggaag cauauccuug     2040

augugcuuag cacuuggagg gguacuaaua uucuugagua cagcaguaag ugcagau        2097


<210>  2533
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2533
agaaaagaga aaaaacgaag aggcgcagau acuaguguag gcaucguagg acuauuauua       60

acaacugcua uggcagcaga aguuacaaga agaggcagcg cauauuacau guauuuagac      120

cgaaaugacg caggagaagc uauuucauuc ccaacaacau uaggaaugaa caaauguuau      180

auacaaauua uggacuuagg ccacaugugu gaugcaacaa ugucauauga auguccaaug      240

uuagaugaag gaguagaacc agaugaugua gacuguuggu gcaauacaac aaguacaugg      300

guaguauacg guacauguca ucauaaaaaa ggugaagcaa gaagaucuag gagagcagua      360

accuuaccau cacauucaac aagaaaauua cagacaagaa gucaaacgug guuagaaagu      420

agagaauaua caaaacaucu aauuagggua gaaaauugga uauucagaaa uccgggauuu      480

gcauuagcag ccgcagcaau agcauggcua uuagggucuu caacaaguca gaaaguaauu      540

uaccuaguaa ugauauuacu aauagcaccg gccuacagua uacgguguau aggaguaagu      600

aacagggauu ucguugaagg aaugagugga gguacauggg uagacauagu auuagaacau      660

gguggaugug uaacuguuau ggcacaagau aaaccuacag uagacauuga acuaguuaca      720

acaacaguua guaacauggc agaaguaagg aguuauuguu augaagcuuc aauaagugau      780

auggcaucag auucacguug uccuacacaa ggagaagcuu auuuagauaa acaaagugau      840

acacaauacg uauguaaaag aacauuagua gaucgaggau ggggaaaugg uuguggucua      900

uucggcaaag guucuuuagu aaccugugca aaauuugcuu gcagcaaaaa aaugacugga      960

aaaaguaucc aaccagagaa cuuagaauau agaauaaugu uaaguguaca uggaagccaa     1020

cacucaggaa ugauaguaaa ugauacagga caugaaaccg augagaauag agcuaaagua     1080

gaaauaacac caaacucacc ucgagcugaa gcaacauuag gaggauucgg aucguuggga     1140

uuagacugug aaccgagaac uggacuagau uuuucugauu uauauuauuu aacaaugaau     1200

aauaaacacu gguuaguuca uaaagaaugg uuucaugaua uaccauuacc uuggcaugca     1260

ggugcugaua cuggaacacc acacuggaau aauaaagaag cauuaguaga auuuaaggau     1320

gcacacgcga aaagacaaac aguaguaguu uuaggaagcc aagagggagc aguacacaca     1380

gcucuagcag gagcguuaga agcagaaaug gauggugcaa aaggacgauu aucaucagga     1440

caucuuaaau gucgacuaaa aauggacaaa cugagacuaa aaggaguauc auauaguuua     1500

uguacagcag cguuuacauu uacuaaaaua ccugcggaaa cacuucaugg aacuguuacg     1560

guagaaguac aauaugcagg aacugaugga ccaugcaaag uaccggccca aauggcagua     1620

gacaugcaaa cuuuaacacc agucggaaga uuaauaacug caaaccccgu aauaacagaa     1680

agcacugaaa acaguaaaau gauguuagaa cuugaucccc cauucggaga uagcuauaua     1740

guuauaggcg uaggagaaaa gaaaauaaca caucauuggc acagaucagg aucaacaaua     1800

ggaaaagcuu uugaagcuac agugagagga gcaaaaagaa uggcaguauu gggagauaca     1860

gcuugggauu ucgguucugu uggcggagcg cuaaauaguc uaggaaaagg aauacaucaa     1920

auauucggug cugcauuuaa aucauuguuc ggaggcaugu caugguuuag ccaaauuuua     1980

auaggaacac uauuaaugug guuaggauua aacaccaaaa auggcucuau uucacuaaug     2040

ugcuuagcau uaggaggugu auuaauauuc uuaaguacag cgguuagugc ggac           2094


<210>  2534
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2534
aaagagaaaa aaagaagagg agcagauacg ucaguaggaa uuguaggauu auuauugaca       60

acagcaaugg cagcugaagu gacucguaga ggaucagcau acuauaugua uuuggaucgu      120

aaugacgcag gugaagcaau uucuuuuccu acaacauuag guaugaauaa guguuauaua      180

caaauaaugg acuuaggaca caugugugau gcaacaaugu cauaugaaug cccuauguua      240

gacgaagggg uagaaccaga cgauguugau uguuggugua auacuacauc aacaugggua      300

guauacggaa caugucacca uaaaaaagga gaagcacgca gaaguagacg agcaguaaca      360

cuaccuucac auucaacaag aaaacuacag acgagaucac aaacuuggcu agaaaguaga      420

gaauacacaa aacaucuaau aagaguagaa aauuggaucu uuagaaaucc agguuuugcg      480

uuagcagcag cagcaauagc uugguuguua gggucgucaa cuagccaaaa aguaauauau      540

cuaguaauga uauuauuaau cgcaccggcu uauaguauaa gaugcauagg aguuaguaau      600

cgagauuuug uagagggaau gucaggagga acuuggguag auaucguucu agaacacgga      660

ggauguguaa caguaauggc acaggauaaa ccaacaguug auauugaauu aguaacaaca      720

acaguaagca acauggcaga aguaaggagu uauugcuacg aagcuaguau aucagauaug      780

gcaagugauu caagaugucc uacacaaggu gaagcauauu uagauaaaca aagcgacaca      840

caauaugucu guaaaagaac auugguugau agagguuggg gaaaugguug cggauuauuc      900

ggcaaagguu cucuaguaac uugcgcaaaa uuugcauguu caaaaaaaau gacagguaaa      960

agcauacaac cagaaaacuu agaauauagg auuauguuau cgguucaugg cucgcaacau     1020

agcgguauga uuguaaauga uacaggacac gaaacagacg aaaacagagc aaaaguagaa     1080

auaacuccaa auagcccaag agcagaagcc acacuaggug gauuugguag uuuaggacua     1140

gauugugaac caagaacagg auuagauuuu agugaccuau auuauuuaac aaugaauaau     1200

aaacacuggu uaguucauaa agaaugguuu cacgauauuc cauuaccuug gcaugcaggu     1260

gcggacacag gaacgccaca uuggaacaau aaagaagcuu uaguagaauu caaagaugcu     1320

caugcaaaaa gacaaacagu aguaguacua ggaagucaag aaggagcggu acauacagca     1380

cuagcaggug cauuagaagc agaaauggau ggagcgaaag gcagacuauc aucuggacau     1440

cuaaaaugua gauuaaaaau ggacaaacua aggcuaaaag gaguaaguua cucacuaugu     1500

acagcagcau ucacauuuac aaaaauucca gccgaaacgu uacacggaac aguaacgguc     1560

gaagugcaau augcaggaac ugauggaccc uguaaaguac cugcucaaau ggcaguagau     1620

augcaaacac uuacaccagu aggaagauua auaacagcua auccaguaau aacagaaucu     1680

acagagaacu caaaaaugau gcucgaauua gacccaccau ucggugauag uuauauagua     1740

auaggaguag gagaaaagaa aauaacacau cacuggcaca gaucagguuc aacaauugga     1800

aaggcauuug aagcuacugu uagaggagca aaacgaaugg caguauuggg ugacacggca     1860

ugggauuuug gaucaguagg aggagcuuug aauucucuag gcaaaggaau acaccaaaua     1920

uucggagcag ccuucaaauc ucuauuugga gguaugagcu gguucucaca aauauuaaua     1980

gguacuuuau uaaugugguu aggauuaaac acuaaaaaug gaucaauauc ccuaaugugu     2040

uuagcguuag gagguguauu aauauuccua aguacagcug ucagcgccga u              2091


<210>  2535
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2535
gaaaaaaaaa gacgaggggc ggauacauca guaggaauag uugguuuauu auuaacaacc       60

gcaauggcag cagaaguaac uagaagagga ucagcauauu auauguauuu agauagaaau      120

gaugcaggag aggcaauauc auuuccuaca acacuuggaa ugaauaaaug uuacauacag      180

auuauggauu ugggacacau gugugacgca acaaugaguu acgaaugccc aaugcuagau      240

gaagggguag aaccagacga cguggauugu ugguguaaca caaccucaac gugggucgua      300

uacggaacau gccaccauaa aaaaggagaa gcccgacgga gucgaagagc agugacauua      360

ccaagucauu caacgagaaa auuacaaacu agaagccaaa caugguuaga aagccgagaa      420

uacacaaaac aucuuauucg aguagaaaau uggauauuua gaaaucccgg guuugcacua      480

gcagcagcag caauagcaug guuacuagga ucaucaacau cccaaaaagu gaucuacuua      540

guaaugauau ugcuuauagc gccagcauau aguauaaggu guaucggagu aaguaauaga      600

gauuucguag aagguaugag uggaggaaca uggguugaca uaguuuugga gcauggcggg      660

ugcguaacag uaauggcaca agacaaaccc acgguagaca uagaauuagu gacuacuacu      720

guaucaaaua uggcagaagu uagaagcuac uguuacgaag caucaauauc ugacauggca      780

agugauagua gauguccaac ccaaggagaa gcauaucuag auaaacaauc agauacacaa      840

uacguaugua aaagaacucu uguagaucga gguuggggaa augguugcgg cuuauuugga      900

aaaggaaguu uaguaacaug ugcaaaauuu gcauguucua aaaaaaugac cgguaaaucg      960

auacagccgg aaaaccuaga auaccgaauu auguuaagug uacaugguuc gcagcauuca     1020

ggaaugauag ugaacgacac aggucaugaa acagaugaaa aucgugcuaa gguagaaauu     1080

acaccaaacu cgccaagagc agaagcaaca cuaggaggau uugguucacu gggauuggau     1140

ugugaaccua gaacaggccu ugauuucuca gauuuauauu auuuaacaau gaauaauaaa     1200

cauuggcuug uacauaaaga augguuucau gauauaccau uaccauggca cgcuggagca     1260

gacacgggua caccacauug gaacaacaaa gaagcacuag uagaauuuaa ggacgcccau     1320

gcaaaaagac aaacuguggu aguacuaggu agucaagaag gagcuguaca uacugcucug     1380

gcaggggcau uagaagcuga aauggauggu gcaaaaggaa gauuaucaag uggacacuua     1440

aaaugcagac uaaaaaugga caaauuaaga uuaaaaggag uuucauauag uuuauguaca     1500

gcagcauuua cuuuuacaaa aauuccagca gaaacauuac auggcacagu aacuguagaa     1560

guccaauaug caggaacaga cggaccuugu aaaguuccag cacaaauggc aguagacaug     1620

caaacguuaa caccaguugg aagacuuaua acugcaaauc caguaauaac agaaaguaca     1680

gagaauagua aaaugaugcu agaauuagau ccaccuuucg gagauucaua uauuguaaua     1740

gguguaggag aaaaaaaaau aacacaccac uggcaucgaa gugguaguac uauaggaaaa     1800

gcauuugaag cuacagucag gggagcaaag agaauggccg uacuaggaga uacagcaugg     1860

gacuucggaa gcguuggagg ugcccuaaau ucauuaggga aagguauaca ccaaauauuc     1920

ggcgccgcau uuaaaagccu auuuggagga augaguuggu uuucacaaau acuaauaggc     1980

acauuauuaa ugugguuagg cuuaaacaca aaaaauggau caauaagucu aauguguuua     2040

gcauuaggag gaguauuaau uuuucuauca acagcuguau cugcugac                  2088


<210>  2536
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2536
aaaaaaagaa gaggagcaga cacaagugua ggaauaguag gacuauuauu aacaacugca       60

auggcagcag aaguaacaag aagaggauca gcguauuaua uguaucucga uagaaaugau      120

gcaggagagg caauauccuu cccaacaacu cuuggaauga acaaaugcua uauacaaauu      180

auggauuuag gacauaugug cgaugcaacc auguccuacg aauguccaau guuagaugaa      240

gguguagaac cugaugaugu agauuguugg uguaauacaa caaguacuug ggugguauau      300

ggaacaugcc aucauaaaaa aggagaggca agaagaagca ggagagcggu aacacuacca      360

ucccauagca cacgaaaacu acaaacuaga agucaaacau ggcuagaaag cagagaauac      420

acuaaacauc uuauaagagu agaaaauugg auauuuagaa acccaggguu ugcgcuagca      480

gccgcagcca uugcuugguu auuaggcuca ucaacaagcc aaaaaguaau auaccuagua      540

augauacuac uaauagcgcc ugcauauuca auuagaugca uuggagucuc caauagagau      600

uuuguagaag gaaugagugg agggacaugg gucgacauag uacuagaaca cggagguugu      660

guuacaguga uggcgcaaga uaaaccaacc gucgauauag aauuaguaac uacaacagua      720

ucaaauaugg cagaaguaag auccuauugu uaugaagcau cgauaagcga uauggccuca      780

gauucuagau gucccacaca aggagaagcu uacuuagaua aacaaucuga uacacaauau      840

gucuguaaaa gaacacucgu agauagagga uggggaaaug gauguggccu auucggaaaa      900

gguaguuuag uaacaugugc aaaauuugca uguagcaaaa aaaugacagg aaaaagcauu      960

caaccagaaa aucuggaaua uagaauaaug uuaucuguac auggaaguca acauucaggg     1020

augauaguua augauacagg acacgaaaca gaugaaaauc gagcaaaagu ugaaauaaca     1080

ccuaauaguc caagagcaga agcaacauua ggaggauuug gaagucuagg auuagacugu     1140

gaaccuagaa cuggauugga uuuuagugac uuauauuauc uuacuaugaa uaauaaacac     1200

uggcuuguuc auaaagaaug guuccacgau auaccacuac cauggcaugc aggcgcugac     1260

acagguacac cccauuggaa uaacaaagaa gcacuaguag aauucaaaga ugcucaugca     1320

aaaagacaaa cagucguagu ucuaggaagu caagaaggag caguacacac agcuuuagcu     1380

ggagcauuag aagcagaaau ggauggugca aaaggacgac uaucaucagg acacuuaaaa     1440

ugcagacuaa aaauggauaa auuaagacua aaaggaguau cauauaguuu guguacagcc     1500

gcauuuaccu uuacaaaaau uccagcugaa acauuacacg gaacuguaac aguggaagua     1560

caauaugcag guacagacgg accguguaag guaccagcuc agauggcagu agauaugcaa     1620

acauuaacgc ccguugguag auuaauaacu gcaaauccag ugauaacaga aucaacagag     1680

aauagcaaaa ugaugcuaga auuagauccc ccauuuggag auagcuauau aguaauugga     1740

guaggagaaa aaaaaauaac acaucauugg caucgaucag gauccacuau agguaaagcu     1800

uuugaagcaa caguaagagg ggcuaaaaga auggcgguau uaggagauac agccugggau     1860

uuuggaucag ucggaggugc auuaaacagu uuagguaaag gaauucauca aauauuugga     1920

gcagcauuca aaagcuuauu uggagguaug ucaugguuuu cgcaaauacu gauuggaaca     1980

uugcuaaugu ggcuaggacu aaauacuaaa aauggaagua ucucauuaau gugucuagca     2040

cuaggaggug uauuaauauu ucucagcaca gcagucucag cagau                     2085


<210>  2537
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2537
aaaagaagag gugcagauac aucaguagga auaguugggu uauuacuaac aacagcaaug       60

gcagcagaag uaacgagaag ggguagugcg uacuauaugu accuagaccg aaaugaugca      120

ggugaagcua uaagcuuucc aaccacacua ggcaugaaua aauguuauau acaaauaaug      180

gaucuaggac auauguguga ugcuacaaug aguuacgaau guccaaugcu agacgaagga      240

guagagccag acgauguaga cuguuggugu aauacgacau caacaugggu aguauauggu      300

acaugucauc acaaaaaagg cgaagcaaga cgaucaagga gagccgucac ucuaccaagu      360

cacagcacaa gaaaacuaca aacuagaagu caaacauggu uagaaucaag agaauauaca      420

aaacaucuaa uaagggugga aaacuggaua uucagaaauc cagguuuugc acuagcagca      480

gcagcgauag cuugguuauu agguaguucu acuucacaaa aaguuauaua cuugguaaug      540

auuuuauuaa uagcacccgc auacaguaua aggugcauug gaguaaguaa uagagauuuu      600

guagagggaa ugaguggagg aacaugggua gauauaguau uagaacaugg aggcugugua      660

acugugaugg cacaagacaa accuacagua gacauugaau uaguaacgac aacaguuuca      720

aauauggcag aaguaagauc uuauuguuau gaagcaagua uaagcgauau ggcaagugac      780

agcagauguc caacacaagg ggaagcuuau cuugacaagc aaucagacac gcaauacguu      840

uguaaacgga cauuaguaga uaggggcugg gggaacggau guggauuauu uggaaaaggg      900

uccuuaguaa caugcgcaaa auucgccugu aguaaaaaaa ugacaggaaa aucuauacaa      960

ccagaaaauc ucgaauauag aauaauguua ucaguacaug gaagucaaca uucagggaug     1020

auaguaaaug auacuggcca ugaaacagac gaaaauagag caaaaguaga aauaacaccu     1080

aauaguccca gagcagaagc aacauuagga ggauucggau cauuaggauu agacugugaa     1140

ccaagaacag gccuagauuu cucugacuua uauuaccuaa caaugaauaa uaaacacugg     1200

cuaguucaca aagagugguu ucaugacaua ccgcuaccuu ggcaugcagg ugcagauaca     1260

ggaacgccac auuggaauaa uaaagaagca cuaguagagu uuaaagaugc ucaugcaaaa     1320

agacaaacgg uaguaguauu aggaagccaa gaaggagcag uacacacagc ucuagcagga     1380

gcucuugaag cagaaaugga uggugcuaaa ggucgauuau cauccggaca cuuaaaaugu     1440

agauuaaaaa uggacaaacu aagauuaaaa ggaguaagcu acagccuaug uacagcagca     1500

uuuacauuua caaaaauacc agcagaaacu uuacauggaa caguaacagu ugaaguacaa     1560

uaugcaggaa cagaugggcc auguaaaguu ccugcgcaaa uggcaguaga caugcaaaca     1620

uuaacaccag uaggaagauu gauaacagca aauccaguaa uaacugaauc aacugaaaac     1680

ucaaaaauga ugcuagaacu agacccacca uucggagaua gcuauauagu aauuggaguu     1740

ggcgaaaaaa aaauaacaca ucauuggcac agaaguggaa guacgauugg aaaagcauuu     1800

gaggcaacag uaagaggugc uaaaagaaug gcaguacuug gagacacagc uugggauuuu     1860

gguagcguag gaggagcauu aaauucacuu ggaaaaggaa uucaucaaau auucggagca     1920

gccuucaaau cacuauuugg aggaaugagu ugguuuucac agauauuaau ugggacacuu     1980

cuaauguggc uaggauugaa uacaaaaaau gggaguauau cuuuaaugug ucuagcauua     2040

ggggguguau uaauauucuu gucaacagcu gugucugccg au                        2082


<210>  2538
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2538
agaagaggcg cagauacauc agucggaauu guuggucuuu uguuaacuac agcaauggca       60

gcagaaguaa caagacgugg aagugcguac uacauguacc uagacagaaa ugaugcagga      120

gaagcaaucu cauuuccuac uacucugggu augaacaaau gcuauauaca aauaauggau      180

cuaggccaua ugugugaugc gacaauguca uaugaguguc caaugcuaga cgaagguguu      240

gaaccugacg auguagauug cugguguaac acaacaucaa cauggguagu auauggaaca      300

ugccaccaua agaaaggaga agcuagaaga ucaagacgag caguaacauu acccagucau      360

aguacaagaa aauuacagac aagaagccaa acauggcuag aaucgagaga auauacuaaa      420

caucuaauaa gaguagaaaa uuggauauuc cgaaacccag gauuugccuu ggcagcagcu      480

gccaucgcau ggcuuuuagg aaguucgaca agccaaaaag uaauauaucu aguuaugaua      540

cuucucauag caccagcuua uucaauaaga uguaucggug uuaguaacag agauuuugua      600

gaagguaugu ccggagguac uuggguugau auaguauuag aacauggugg auguguuacu      660

guaauggcac aagauaaacc aacaguagau auagaauuag uaacaacuac aguaagcaac      720

auggcagaag uaagaucuua uuguuaugaa gcaucaauau cagauauggc aagugauagu      780

agaugcccaa cccaaggaga agcauacuua gauaaacagu cagauacaca auauguaugu      840

aaacgaacuu uaguugaucg agggugggga aacggaugug guuuauuugg aaaaggaagu      900

uuaguaacau gugcuaaauu ugcaugcuca aagaaaauga caggaaaauc aauacaacca      960

gaaaauuuag aauauaggau aaugcuuagc guacauggau cacaacauuc cggaaugauu     1020

guaaaugaca cgggacauga aacugaugaa aacagagcua aagucgaaau aacaccuaau     1080

aguccucgag cagaagcaac auuaggagga uuugguagcu uaggacuuga cugugaaccu     1140

agaacaggac uagauuuuag ugaucuauac uaccuuacca ugaauaauaa acauuggcua     1200

guacacaaag aaugguuuca ugacauacca uuaccauggc augccggagc agacacaggu     1260

acaccucacu ggaauaauaa agaagcccua guagaauuua aagaugcaca cgcaaaacga     1320

caaacagugg uggucuuagg aagccaagaa ggagcaguac auacagcauu agccggugcu     1380

cuagaagcag aaauggaugg ugcaaaagga agacuuagcu caggacauuu aaaauguagg     1440

uuaaaaaugg auaaauuaag guuaaaagga guaucauaua gucuaugcac agcagcauuu     1500

acuuucacaa aaauacccgc cgaaacgcua cacggaacag uaacaguaga aguacaauac     1560

gcaggaacug augguccaug uaaaguacca gcucaaaugg caguagacau gcaaacauua     1620

acaccaguag guagguuaau aaccgcgaau ccaguaauaa cugaaucaac agaaaauagc     1680

aaaaugaugu uagaacuaga cccuccauuc ggagauagcu auauaguaau agguguagga     1740

gagaagaaaa ucacacauca uuggcauaga aguggaucaa caauaggaaa agcauuugaa     1800

gcuacaguaa gaggagcaaa acguauggca guauugggag auacugcaug ggauuuugga     1860

aguguagggg gagcucuaaa uucacuuggc aaaggaauac aucaaauauu uggagcagca     1920

uuuaaauccu uauucggagg aaugucuugg uuuagccaaa uacuaauagg aacuuuauua     1980

auguggcuug gacuaaacac uaaaaaugga ucaauuucuu uaaugugucu agcucuagga     2040

ggaguacuua ucuuucuaag uacagcagug agugcagac                            2079


<210>  2539
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2539
agaggagcag acacaagugu agguauaguu ggauuauuau uaacaacagc aauggcagcc       60

gaaguaacaa gaagagguuc agcuuauuau auguauuuag acagaaauga ugcaggagaa      120

gcaauaucau ucccaaccac gcuuggaaug aauaaaugcu auauacaaau aauggauuua      180

ggacacaugu gugaugcaac aaugucauau gaaugcccca uguuagauga agguguagaa      240

ccagaugacg uagacuguug guguaauaca acgagcacau gggucguaua uggaacuugc      300

caucauaaga aaggagaagc uagacgaagc agacgagcag uaacgcuacc aagucacuca      360

accagaaaau ugcaaacaag gagucaaaca uggcuagaau caagagaaua uacuaaacau      420

cuuaucagag uagaaaauug gauuuuucga aauccaggau uugcacuugc ugcagcugcu      480

auagcauggc uauuagguuc aagcaccuca caaaaaguaa uauaucuagu uaugauacua      540

uuaauugccc cugcauacuc aauuagaugu auaggaguau caaauagaga cuucguagag      600

ggaaugucag gaggaacuug gguagauaua guacuagaac augguggaug uguaacagua      660

auggcacaag auaaaccaac uguugacaua gaauuaguaa caacaaccgu aaguaauaug      720

gcagaaguaa gauccuacug cuaugaggca aguauaagcg auauggcauc agauagucga      780

uguccaacac aaggggaagc auauuuagau aaacaaucag auacgcaaua cgucuguaaa      840

agaacauuag uggacagagg augggguaau gguuguggac uauucggaaa agguagcuua      900

guuacaugug caaaauuugc auguaguaag aaaaugacag gaaaaucaau ucaacccgaa      960

aacuuagaau auagaauaau gcuaucagua cauggaaguc aacauucagg uaugauugua     1020

aacgauaccg gccaugaaac cgaugaaaau agagcuaaag uagaaauaac gccuaauagc     1080

ccaagagcgg aagcaacacu gggaggauuu gggagcuuag gauuggauug ugaaccaaga     1140

acaggacuag auuucucuga cuuauauuac cuaaccauga auaauaaaca uugguuagua     1200

cauaaagaau gguuucauga uauaccacua ccguggcaug caggggcaga uaccggaacc     1260

ccacacugga auaauaaaga agccuuagua gaauuuaaag acgcgcaugc aaaaagacaa     1320

acuguaguag uauuaggauc acaagaaggg gcaguucaca ccgccuuagc aggagcacua     1380

gaagcagaaa uggauggagc uaaaggucga cuaucaucag gacauuuaaa auguagacuu     1440

aaaauggaua aauuaagauu aaaaggaguu aguuacucau uaugcacugc agcauucacu     1500

uucaccaaaa uaccagcaga aacauuacau ggcacuguaa caguagaggu acaauaugcc     1560

ggaacagaug gacccugcaa aguaccagcg cagauggcug ucgauaugca aacauuaacu     1620

ccaguugguc gacucauaac ugcuaauccc guaauaacag aaucaacaga aaauucaaaa     1680

augaugcuug aauuagaucc ucccuuugga gauucauaua uaguaauagg aguaggggaa     1740

aagaaaauaa cccaccauug gcauagaagu ggaaguacaa uugguaaagc auuugaagca     1800

acaguuagag gagcaaaaag aauggcggua uuaggagaua cagcguggga cuucggcagc     1860

guaggaggcg ccuuaaauuc uuuaggaaaa gggauacauc aaauauuugg agcagcauuc     1920

aaaucauuau ucggaggaau gucaugguuu ucacaaaucu uaauaggaac ucuauuaaug     1980

ugguuagguc uaaauacuaa aaacggcuca auaaguuuaa uguguuuggc acuaggcgga     2040

guacuaauuu uccuaucgac agcaguaucc gcagac                               2076


<210>  2540
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2540
ggagcggaua caucaguugg uauaguagga cuguuauuaa caacagcuau ggcugcagaa       60

guaaccagaa gaggaucagc auauuauaug uauuuagaua gaaaugaugc uggagaagcu      120

auaucauuuc ccaccaccuu aggaaugaau aaauguuaca uucaaauaau ggauuuggga      180

cacaugugcg augcaacaau gagcuaugag ugucccaugu uagacgaagg aguugaacca      240

gaugacguag auuguuggug uaauacgaca aguacuuggg uaguauacgg cacaugucau      300

cacaagaaag gagaagcacg ccgcucaaga cgagcuguaa cccuucccag ucauaguaca      360

aggaaauugc aaacucguag ccaaacaugg uuagaaucca gagaauauac caaacaucuc      420

auaagaguag aaaacuggau auuuagaaau ccaggauucg cauuagcugc agcagcaaua      480

gcauggcucc uaggaucaag cacaucccaa aaaguuauau accuaguuau gauauuauua      540

auagcuccag cauauucaau uagauguaua ggaguuucaa accgugacuu uguugaaggc      600

augucaggag gaacaugggu agauauagua cuagaacacg gaggaugugu aacuguaaug      660

gcacaagaca aacccacagu ugauauagag cuaguuacua caacaguaag uaauauggca      720

gaaguaagaa guuauugcua cgaagcauca auaagugaua uggcaaguga caguagaugc      780

ccaacccaag gagaagcgua cuuagacaaa caaagugaua cccaauaugu uuguaaaaga      840

acacuuguug aucgaggaug gggaaaugga ugcggacucu uugguaaagg aucauuagua      900

acaugugcaa aauuugccug uagcaagaaa augacaggaa aaucaauaca accagaaaac      960

cuagaauaua gaauaauguu aucuguacau gguucacaac auucagguau gauugucaac     1020

gacacaggac augaaacuga ugaaaauaga gcaaaagucg aaauaacucc aaauucacca     1080

cgagcagaag ccacuuuagg ggguuuugga agucuaggau uagacuguga accaaggaca     1140

ggauuagauu uuucagaucu auauuauuua acaaugaaca acaaacauug gcuaguacac     1200

aaagaauggu uucaugacau accauuaccc uggcacgcag gcgcugacac uggaacuccg     1260

cacuggaaua auaaagaagc ccuaguugag uucaaagacg cacaugcaaa aagacaaaca     1320

guaguuguau uaggaucaca agaaggagcu guacauacag cauuggccgg agcauuagaa     1380

gcugaaaugg auggagcaaa agguagacua agcagcggcc aucucaaaug cagauuaaaa     1440

auggacaaac uaagacuaaa aggcguuuca uacucacuau guacagcagc uuuuacauuu     1500

acuaaaauac cugcagaaac auuacauggu acaguaacag uugaaguaca auaugcagga     1560

acagauggac caugcaaagu accagcacaa auggcagucg auaugcagac auuaacgcca     1620

guaggacgac uaauaacagc aaauccugua auaacagaaa gcacagaaaa uucaaaaaug     1680

augcuagaac ucgacccucc cuucggggau ucauauauag uaauaggagu aggcgaaaag     1740

aaaauaacac accauuggca ucguucagga aguacuauag gaaaagcauu cgaagccaca     1800

guaagaggug caaaaaggau ggcaguguua ggagauacag caugggauuu uggaucaguc     1860

ggaggagcac uaaauagcuu aggaaaagga auacaucaga uauuuggagc agcauuuaaa     1920

aguuuauucg guggaauguc uugguucucu caaauuuuaa uaggaaccuu acuuaugugg     1980

uuggggcuaa auaccaaaaa uggaucaaua ucauuaaugu gcuuagcucu aggaggugua     2040

cuaauauuuc uaucgacagc aguguccgca gau                                  2073


<210>  2541
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2541
gcugauacau caguaggcau aguaggucua cuuuuaacaa cagcaauggc agcugaagua       60

acaaggcgag guagcgcuua uuacauguac uuagauagaa augaugcagg ggaagcuaua      120

ucuuuuccga caacauuagg aaugaauaaa ugcuauauuc aaauaaugga ccugggacau      180

augugugaug cgacaaugag uuaugaaugc ccaauguuag augaaggagu ugaaccggau      240

gacguagacu gcugguguaa uacaacaagu acaugggucg uauaugguac augucaucau      300

aaaaaaggag aagcaagaag aucaagaaga gcaguuacau uaccuucaca cucuacaaga      360

aaauugcaaa cacgaagcca aacguggcuu gaaucaagag aguacacaaa acauuuaauc      420

cgaguagaaa auuggauuuu cagaaaucca ggauucgcac uagccgcagc agcaauagca      480

ugguuacucg gaucaaguac aucacaaaaa guaauuuacu uaguaaugau auuacuaaua      540

gccccagcau auaguauaag auguauagga guaaguaaua gagauuuugu ggaaggaaug      600

aguggaggua cguggguaga uauuguauua gaacauggug gauguguaac aguaauggca      660

caggauaaac caacaguaga cauugaauua guaacaacaa ccguaucaaa cauggcagaa      720

guaagaucau acuguuauga agcaaguaua ucugauaugg cgagcgacuc aagauguccu      780

acacaaggug aagcguaucu agauaaacaa uccgauacuc aauauguaug uaaaagaacc      840

cuaguagaca gagggugggg caaugguugu gguuuauuug gcaaaggauc auugguaaca      900

ugugcaaaau ucgcaugcuc aaaaaaaaug acuggaaaau caauucagcc ggaaaaccua      960

gaauauagaa uaauguuauc uguucacgga agucaacacu caggaaugau uguaaacgau     1020

acuggucacg aaacugauga aaauagagcu aaaguagaaa uaacaccaaa uaguccaaga     1080

gcagaagcca cccuuggugg auucggaagu cuaggauuag auugcgaacc aaggaccgga     1140

uuagacuucu cagauuuaua uuaucuaacu augaauaaua aacauuggcu aguacacaaa     1200

gaaugguuuc acgauauacc uuuaccaugg caugcaggag cagauaccgg uacaccacac     1260

uggaacaaua aagaagcuuu aguagaauuu aaagaugcuc acgcaaaacg acaaacgguu     1320

guaguacuag gaucacaaga aggagcaguc cacacagcau uagcgggagc cuuagaagca     1380

gaaauggaug gagcaaaagg aagacuuagu ucaggacauu uaaaaugccg auuaaaaaug     1440

gauaagcuua gacuaaaagg aguaaguuac agccugugca cagcugcauu uacauuuaca     1500

aaaaucccag cggaaacauu acauggaacc gucacaguag aaguacaaua cgccggaaca     1560

gauggcccau gcaaaguacc agcucaaaug gcuguagaua ugcaaacacu aacaccagua     1620

ggaagacuaa uaacagccaa cccaguaaua accgagucua cagaaaacag uaaaaugaug     1680

uuagaacucg auccuccauu uggagauagc uauauaguaa uugguguagg agaaaaaaaa     1740

auaacacauc auuggcaucg aucagguuca acaauaggua aagcauuuga agcuacagua     1800

cgaggagcca aaagaauggc aguauugggu gauacagcau gggauuuugg aucugucgga     1860

ggagcauuaa acucauuagg aaaagguaua caucaaauau uuggugcugc auucaaaagc     1920

uuauuuggag gaaugucaug guuuucccaa auucuaauag gaacauuauu aaugugguua     1980

ggauuaaaca cgaaaaaugg gucaauaagu cuaaugugcc uugcauuagg uggaguacua     2040

auauuucuaa gcacugcagu aucagcugau                                      2070


<210>  2542
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2542
gacacaagug uaggaauagu aggauuguua uuaacaacug caauggcagc agaagucacu       60

cgucguggua gcgcauacua uauguauuua gauagaaaug augcaggaga agcaauaucu      120

uuuccgacaa cauuaggaau gaacaaaugu uauauacaaa uaauggauuu aggucauaug      180

ugugacgcua cgaugucaua ugaaugucca auguuggaug aaggaguaga accagaugac      240

gucgauuguu ggugcaauac gacauccaca uggguaguuu augggacaug ucaucauaag      300

aaaggagaag cgaggagaag caggagagca guaaccuuac caagucacag cacaagaaaa      360

cuacaaacaa gaagucaaac augguuagaa ucaagagaau auaccaaaca cuuaauaagg      420

guggaaaauu ggauauuucg aaauccagga uuugcauuag cagcagcugc aaucgcgugg      480

uuauuaggau caucaacauc acagaaagua auauaccuag uuaugauauu auuaauagca      540

ccagcauaua gcauaagaug cauaggagug aguaauagag auuuuguaga aggaaugagu      600

ggaggcaccu ggguagauau aguacuagaa cauggcggau gugucaccgu uauggcucaa      660

gauaaaccaa caguagauau agaauuagua acuacaacag uaucaaauau ggcagaagua      720

agaaguuacu gcuaugaggc uucaauaagu gacauggcuu cagauucaag auguccgaca      780

caaggagaag cauauuuaga uaaacagucu gauacucaau auguaugcaa aagaacccua      840

guagacagag gauggggaaa ugguuguggu uuauucggaa aaggaagucu gguuacaugu      900

gcaaaauucg caugcucuaa gaaaaugaca ggaaaaucaa uccaaccaga aaaccuagaa      960

uacagaauaa uguuaucagu acacggcuca caacauagug gaaugauagu gaaugauacu     1020

ggacacgaaa cugaugaaaa ucgagcaaaa guagaaauaa cacccaacag uccgagagca     1080

gaagcuacau uaggaggauu ugguucauua ggauuggauu gcgaaccaag aacagguuua     1140

gacuuuagug auuuauauua ucuuaccaug aauaacaaac auuggcuugu acauaaagaa     1200

ugguuucaug acauuccucu accauggcau gccggagcag auacggguac uccacacugg     1260

aauaauaaag aagcauuggu agaauuuaaa gaugcacaug caaaaagaca aacuguagua     1320

guauuaggaa gucaagaagg agcaguucau acugcguuag cuggagcauu agaagccgaa     1380

auggauggag caaagggaag auuaucaagu ggacaccuua aaugccgacu gaaaauggau     1440

aaacuaagac uuaaaggugu uucuuauucu uuauguacug cggcuuucac auuuacgaaa     1500

auacccgcug aaacacuuca ugguacugua acaguagaag uacaauaugc aggaacagac     1560

ggaccaugua aaguaccagc acaaauggca gucgauaugc aaacacuuac accaguaggu     1620

agacuuauca cagcgaaucc aguuauaacu gaaaguacag aaaauaguaa aaugaugcug     1680

gaauuagauc cuccuuucgg agacaguuac auaguuauug guguagggga aaagaaaaua     1740

acacaucacu ggcauagaag uggaucaacu auaggaaaag cauuugaagc aacaguccgu     1800

ggagcaaaac gaauggcagu acuaggcgau acugcauggg auuuugguag cguuggugga     1860

gcacuaaauu cacuuggaaa aggaauacac caaauauuug gagcagcauu uaaaucacuu     1920

uuugguggca ugucaugguu uucacaaauu uuaauuggaa cauuauuaau guggcuagga     1980

uuaaauacua aaaacgguag uauaucguua augugucuag cacuaggugg agucuuaaua     2040

uuucuuagua cagcaguuag ugcagau                                         2067


<210>  2543
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2543
acaagugucg gaauaguagg auuauuguua acaacggcaa uggccgcaga agugacgaga       60

agggggucug cuuauuauau guaucuugau agaaaugaug caggagaagc uauaaguuuu      120

ccaacaacuc uaggaaugaa uaaauguuac auucagauaa uggacuuagg gcauaugugc      180

gaugcaacaa ugucauacga gugcccaaug cuagaugaag gaguagaacc agaugacguu      240

gacugcuggu guaauacaac uucaacaugg guagucuacg guacauguca ucacaaaaaa      300

ggagaagcaa gaagaucuag aagggcagug acauuaccua gucauagcac gaggaaacuc      360

caaacccgau cacaaacaug gcuagaaagc agagaauaua caaagcaccu aauaagagua      420

gaaaauugga uuuuuagaaa uccaggauuc gcauuagcag ccgcugcgau ugcaugguua      480

cuuggaucaa guacgucgca aaaaguuaua uacuuaguaa ugauauugcu aaucgcacca      540

gcauauucga ucagaugcau aggaguauca aacagagacu ucguagaagg aaugagugga      600

ggaacauggg uagauauagu acuagaacau ggagguugug ugacaguaau ggcacaagau      660

aaaccaacug uggacauaga acuagugaca acaacaguau caaacauggc agaaguacga      720

ucuuacuguu augaagcguc aauaagcgau auggcaagcg auaguagaug cccaacacaa      780

ggagaagcau acuuagauaa acaaucagau acucaauaug uauguaaacg aacacuagua      840

gauagaggau ggggaaaugg auguggauua uuuggcaaag gaagucuggu aacuugugca      900

aaauuugcuu gcaguaaaaa aaugacagga aaaaguauac aaccagaaaa ccuugaauau      960

aggauuaugu uaaguguuca cgggagucaa cauaguggga ugauugucaa ugauacagga     1020

caugaaacag acgaaaauag agcaaaaguc gaaauaacgc caaacagucc aagagcugaa     1080

gcgacauuag gaggauuugg aucauuagga cuagauugug aaccaagaac ugggcuagac     1140

uuuucagacu uauacuaucu aacaaugaau aauaagcacu gguuaguuca uaaagaaugg     1200

uuucaugaca uaccacuacc uuggcaugca ggagcagaua cuggaacucc gcauuggaau     1260

aauaaagaag cauuaguaga auucaaagau gcacaugcaa aacgacagac aguagucgua     1320

cuuggaaguc aagaaggagc ggugcauaca gccuuagcag gagcauuaga agcagagaug     1380

gacggcgcua aagguagacu uagcagcgga caucuaaaau guagacuaaa aauggauaaa     1440

cuaagacuaa aaggaguauc auauucauua uguaccgcag cguucacauu uacaaaaaua     1500

ccagcggaaa cauuacaugg aacuguaaca guagaaguac aauaugcagg aacugaugga     1560

ccauguaaag uaccagcaca aauggcagua gauaugcaaa cauuaacacc cguaggaaga     1620

cuaauaacag caaauccagu aauaacagaa aguacagaaa auucuaaaau gauguuggaa     1680

uuagacccac cuuuuggaga caguuacaua guaauaggag uaggagaaaa aaaaauaaca     1740

caccauuggc auaggagugg aagcaccaua ggaaaagcau uugaagcuac uguaagagga     1800

gcgaaacgaa uggcuguucu aggagauacg gcuugggacu uuggaagugu aggaggagcg     1860

uuaaauaguc ucggcaaagg aauucaucaa auauucggag cagcauuuaa aucacuauuu     1920

gguggaaugu caugguuuag ucaaauauug auaggaacac uauuaaugug guuaggauua     1980

aauacuaaaa acggcaguau aucacuaaug ugcuuagcau uagguggagu uuuaauauuc     2040

cuaaguacag caguaagugc agau                                            2064


<210>  2544
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2544
acaucaguag gaauuguugg uuuacuauua acaacagcaa uggcagccgc aguaacacgc       60

cgaggcucgg cuuacuauau guauuuagau agaaacgaug caggagaagc aauaucauuu      120

ccaaccacac uggguaugaa uaaaugcuau auccaaauaa uggaucuagg acauaugugc      180

gacgcaacaa ugucauauga auguccaaug cuagaugaag gagucgaacc agaugaugua      240

gauugcuggu guaauaccac aaguaccugg guaguauaug gcacauguca ucauaagaaa      300

ggcgaagcaa gaagaaguag acgagcagua acauuaccau cacauucaac aagaaaacuu      360

caaacaagaa gccaaacuug gcuagaaagc agagaauaua caaaacauuu aauaagagug      420

gaaaauugga uauucagaaa uccuggcuuu gcauuagcug cagcggcuau agcuugguua      480

cuuggaucua guacuucaca gaaaguaaua uaucuaguaa ugauacuauu aauagcacca      540

gcuuacagua uccgaugcau aggaguaucg aacagagauu uuguagaagg gaugucaggu      600

ggaacauggg uagauauagu auuagaacac ggcggaugcg uaacaguaau ggcacaagac      660

aaaccaacag uagacauaga acuuguaaca acaacaguau caaauauggc agaaguuaga      720

ucauacugcu augaagcauc aauaucagac auggcuucag auaguagaug uccaacacaa      780

ggagaagccu acuuagauaa acaguccgau acucaauaug uauguaaaag aacccuagua      840

gaccgcgguu ggggaaaugg uugcggacua uucggaaaag gaagucuagu aaccugugca      900

aaauuugcau guaguaagaa aaugaccgga aaaaguauuc aaccagaaaa uuuagaauau      960

agaauaaugu uaaguguaca uggcucucaa cauucaggaa ugauagucaa ugauacaggu     1020

caugaaacag augaaaauag agcuaaaguc gaaauaacac caaauucacc acgugcugaa     1080

gcaacacuag gaggauuugg aucacucgga uuagauugug aaccaaggac aggcuuggau     1140

uucucagauu uauauuaucu aacuaugaac aacaaacauu gguuagucca uaaagaaugg     1200

uuucacgaua uaccucuacc auggcaugcu ggugcagaua caggaacacc ccauuggaau     1260

aauaaagaag cucuagucga auuuaaagau gcacacgcaa aacgccagac aguaguugua     1320

cuaggaagcc aagaaggagc aguacacaca gcucuagcag gagcuuuaga agcagaaaug     1380

gauggugcaa aaggaagacu aagcucagga caucugaaau gcaggcuaaa aauggauaaa     1440

uuaagauuaa aaggaguaag cuauucauua uguacagcag cauuuacauu cacaaagaua     1500

ccagcggaaa cauuacacgg gaccguaacc guagaaguac aauaugcagg uacagacgga     1560

ccauguaaag uaccagcaca aauggcagua gacaugcaaa ccuuaacacc aguaggucga     1620

uuaauaacag ccaauccagu aauaacagaa ucaaccgaaa auuccaaaau gaugcuagaa     1680

uuagauccac cuuucggaga uaguuauaua guaauaggag uaggagaaaa gaaaaucaca     1740

caucacuggc auagaucugg cuccacuaua ggaaaagcau uugaagcuac cguuagagga     1800

gcaaaaagaa uggcugucuu aggagauacu gcaugggacu uuggaagugu uggaggugca     1860

cuuaacagcc uaggaaaagg aauacaucaa auauucggug cagcuuuuaa aagucucuuc     1920

gguggaaugu ccugguucuc ucaaauuuua auagguacac uacuuaugug gcuuggucua     1980

aacaccaaaa auggaucaau aucauuaaug ugccuggcac uaggaggagu acuaauauuc     2040

uuaucaacag cuguaagcgc agau                                            2064


<210>  2545
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2545
agaggagcag auacaagugu ugguauagua ggcuuacuau uaacaacagc aauggccgcu       60

gcuguaacac gaagaggaag ugcauauuac auguacuuag accgaaacga ugcaggagaa      120

gccauaucau uuccaacaac guuaggaaug aacaaaugcu acauacagau aauggaucua      180

ggacauaugu gugacgcaac gaugucauac gaauguccaa uguuagauga aggcguagaa      240

ccagaugaug uagauuguug gugcaacaca acaaguacau ggguaguaua uggcacaugu      300

caccauaaaa aaggggaagc cagaagaagc agaagagcgg uaacacuacc uucacauagu      360

acuagaaaac uucaaacgcg cucucaaaca uggcuugaga guagagagua uacaaaacau      420

uuaauuagag uagaaaauug gauauuucga aaucccgguu uugcauuagc agcagcagca      480

auagcauggc ugcucggauc aucaacuuca caaaaaguua uauauuuagu aaugauauua      540

cuaauagcac cagcauacag cauacgaugc auaggaguuu caaacagaga uuuuguagaa      600

ggaaugucug gaggaaccug gguagacauu gugcuugagc auggaggaug cguaacuguu      660

auggcacagg acaaaccaac gguugauauc gaauuaguua caacuacagu aucaaauaug      720

gcggagguua gauccuauug uuaugaggca ucuauaucag auauggcauc agauucgaga      780

uguccuacuc aaggugaagc uuaucuagac aaacaaagug acacgcaaua uguuuguaaa      840

agaacuuuag uugacagagg auggggaaau ggcugugguu uauuuggaaa agggaguuua      900

guaacaugug caaaauuugc cuguucuaaa aaaaugacag gcaaaucaau acaaccagaa      960

aauuuagaau auagaauaau guuaucagua cauggaaguc aacauagcgg aaugauaguu     1020

aaugauacag gacacgaaac cgaugaaaac cgugcuaaag uugaaauaac accaaauuca     1080

ccaagagcag aagcaacauu aggaggauuc ggauccuuag gacuugacug ugaaccaaga     1140

acaggacuag acuuuaguga cuuauauuau uugacaauga auaauaaaca uugguuagua     1200

cauaaagaau gguuucacga uauaccauua ccuuggcacg caggagcaga uacaggcaca     1260

ccacauugga auaacaaaga agcuuuagua gaauucaaag acgcacacgc aaaacgacaa     1320

acuguugucg uacuaggauc acaagagggu gcaguacaca cagcgcuagc uggagcauua     1380

gaagcagaaa uggacggagc uaaaggacgc uuaucaucag ggcaccuaaa augucgacua     1440

aaaauggaua aacuaagguu aaaaggagua uccuauuccc uauguacagc agcauucaca     1500

uucacaaaaa uaccagcaga aacauuacac ggaacaguua cuguggaagu acaauaugca     1560

ggaacagaug gaccauguaa aguuccagcu caaauggccg uagacaugca aacguuaaca     1620

ccaguaggaa gacuuauuac agcgaaccca guuauaacag aaaguacuga aaauaguaaa     1680

augauguugg aauuagaucc uccauuugga gauucuuaua uaguaaucgg cguaggggaa     1740

aaaaagauaa cacaucauug gcacagaagc ggaucuacaa ucggaaaagc auuugaagca     1800

acaguaagag gcgcaaaaag aauggcagua cuaggagaua ccgcauggga uuuugguagu     1860

guaggaggag cacuaaauag ccuaggaaaa ggaauacacc aaauauucgg ggcagcauuc     1920

aaaagcuuau uugguggaau gucaugguuc agccaaauac uaauaggaac auuauuaaug     1980

uggcuuggac ucaacaccaa aaauggaucu auuagccuaa ugugcuuagc guuaggagga     2040

guacuaauau ucuuaucaac agcaguaagu gcagau                               2076


<210>  2546
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2546
cgaggauuau caucaaggaa acgaagaagc caugacgucu uaacuguaca auucuuaaua       60

cuaggaaugc uauuaaugac aggcggugua acaagaagag gaagugcaua cuacauguau      120

cuugacagaa acgaugcggg ugaagccaua agcuuuccaa caaccuuagg aaugaauaaa      180

uguuacauac aaauuaugga cuuagggcau augugcgaug caacaaugag uuaugaaugu      240

ccuaugcuag augagggagu agaaccugac gauguagacu guuggugcaa cacaacauca      300

acuugggucg uuuaugggac uugucaccau aaaaaaggug aggcaagaag aaguagaaga      360

gcaguaacau uaccuucaca uuccacaaga aaauugcaaa cacgaucaca aacaugguua      420

gaaaguagag aauacacaaa acaccuuaua agagucgaaa auuggauauu ucgaaaucca      480

ggcuuugccu uggcugcugc agcaauugcc uggcuauuag guaguucuac uagucaaaaa      540

gugauauauu uagugaugau uuuauugaua gcaccggcau auagcauaag auguauuggc      600

guaaguaaua gagauuuugu ugaagguaug agugguggua ccuggguaga uauaguccua      660

gaacauggag gauguguaac aguaauggca caagauaaac caacaguaga uaucgaacua      720

guaacuacaa cugucucaaa cauggcagag guuagaaguu auuguuacga agcaaguaua      780

agugauaugg caagcgauuc ucgaugucca acacaaggug aagcuuacuu agauaaacaa      840

ucagauacac aauauguaug uaaaagaaca cuaguugaca gaggaugggg uaauggaugu      900

ggacuauuug gaaaaggauc cuuagucacu ugcgcuaaau uugccuguag caaaaaaaug      960

acggguaaau caauacaacc cgaaaauuua gaauaccgaa uuauguugag uguucaugga     1020

ucucaacauu cagguaugau uguaaaugau acaggacaug aaacagauga aaaccgagca     1080

aaaguagaaa ucacuccaaa cagcccuaga gcagaggcaa cccuuggagg auucggaagu     1140

uuaggccuag auugugaacc aagaacagga uuagauuuua gugacuuaua cuaccuaaca     1200

augaauaaua aacauugguu aguacauaaa gaaugguuuc augauauacc cuuaccuugg     1260

caugcuggag cagacacagg aacgccacau uggaauaaca aagaagcacu aguugaauuu     1320

aaagaugcac augcaaaaag acaaacagua guaguauuag gaagucaaga gggagcagug     1380

cacacagcuu uagcaggcgc acuagaagcu gaaauggacg gugcaaaagg uagacuauca     1440

aguggacacu uaaaauguag acuaaaaaug gacaaauuaa gauuaaaagg ggugucauau     1500

ucuuuaugua cagcugcauu uacauucacu aaaauuccag cagaaacacu acauggaaca     1560

guaacagugg aaguacaaua ugcaggaaca gauggaccau gcaaagugcc ugcacaaaug     1620

gcaguagaua ugcaaaccuu aaccccagua ggaagauuaa uaacggcaaa cccgguaaua     1680

accgaaucaa cugaaaacag uaaaaugaug uuggaacuag auccaccauu uggagauuca     1740

uauauaguua uaggaguggg agaaaaaaaa auaacccauc auuggcauag aaguggaagc     1800

acaauaggaa aggcauucga agcuacaguc agaggagcaa aaagaauggc uguucuugga     1860

gauacagcau gggauuuugg uaguguuggu ggagcuuuaa auagccuugg aaaaggaauu     1920

caucaaauau uuggagcagc auucaaaagc uuauuuggag gaaugucuug guuuucacaa     1980

auauugauag gaacacuuuu aaugugguua ggauugaaca caaagaacgg aaguauaucu     2040

uuaauguguu uagcauuagg ugguguucua auuuuucuau caaccgcagu aagugcagau     2100


<210>  2547
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2547
agaggauuga guaguagaaa acgacgaagu cacgauguuc uaacaguaca auuucuaauu       60

uuagguaugc uauugaugac aggagcagua acaagacgag gaucagcaua uuauauguau      120

uuagauagaa augaugcagg agaagcaaua aguuuuccaa cuacuuuagg aaugaacaaa      180

uguuauauuc aaauaaugga uuuaggccau augugugaug cuacaaugag uuaugaaugc      240

ccaauguuag acgaaggagu agaaccagac gacguagauu guugguguaa uacaacauca      300

acauggguag uguauggcac augucaucac aagaaaggag aagcgagaag aaguagacga      360

gcaguuacac uaccaaguca uagcacgaga aaauuacaaa caagaucaca gaccugguua      420

gaaucaagag aauacacuaa acaccugaua agaguagaaa auuggauuuu uagaaauccc      480

ggauuugcac uagcagcugc agcuauagcu uggcuauuag guaguuccac uagccaaaaa      540

guaauuuacc uaguaaugau auuacuaaua gcaccagcau auaguauaag augcauagga      600

gugucaaaua gagauuuugu agaaggaaug agcgguggua caugggugga uauagucuua      660

gaacauggag gauguguaac cguaauggca caagacaaac caacaguuga uaucgaacuu      720

guaacaacaa cgguaucaaa uauggcugaa guaagaucau acuguuauga agcaaguaua      780

ucagauaugg caagugacuc aagaugccca acacaaggag aagcauaucu agauaaacaa      840

ucagacacac aauaugugug caaaagaaca uuaguagaca gaggaugggg aaauggaugu      900

ggacuauucg gaaaagggag cuuaguaaca ugcgcuaaau ucgcgugcag uaagaaaaug      960

acaggaaaaa guauacaacc agaaaauuua gaauauagaa uaaugcuauc aguacacgga     1020

ucgcaacauu cagguaugau aguaaacgau acaggacaug aaacagauga aaaccgagcu     1080

aaaguggaaa uaacgccaaa uucacccaga gccgaagcaa cauuaggggg uuucggaagu     1140

uugggauugg auugcgaacc uagaacgggg uuagauuuuu cagauuuaua uuaccuaaca     1200

augaauaaua aacauuggcu cguacacaaa gaaugguuuc augauauacc uuuaccaugg     1260

caugcaggag ccgauacugg uacuccacau uggaauaaua aagaagcccu aguagaauuu     1320

aaggaugcuc acgcaaaaag acaaacaguu gugguucuag gaagucaaga aggagcggua     1380

cauacagcau uagcaggcgc auuagaagcu gaaauggaug gagcgaaagg ucgacugagu     1440

ucaggacauc uaaaauguag auuaaagaug gauaaacuaa gacuaaaagg aguaucauau     1500

ucauuaugua cugcagcuuu uacguuuacc aagauuccag cagaaacacu acauggaacu     1560

guuacaguag aaguucaaua ugcuggaaca gacggaccau gcaaaguacc cgcacagaug     1620

gcuguagaca ugcagacauu aaccccagua ggacgacuaa uaacggcuaa cccagucauc     1680

acugaaucaa cugaaaauuc gaaaaugaug cuagaacuag acccaccuuu uggagacagu     1740

uauauaguua uuggaguugg agaaaagaaa auaacacauc acuggcauag aagcggaagu     1800

acaauaggua aagcauuuga agcaacagua cgcggugcaa aaagaauggc aguuuuagga     1860

gauacugcau gggauuucgg aucuguaggu ggagcacuaa acucucuugg aaaagguauu     1920

caucaaauau uuggagccgc uuuuaaaagu uuguuuggag gaaugagcug guuuucacag     1980

auauuaaucg gaaccuuacu aauguggcua ggucuaaaua ccaaaaaugg aucaauaagu     2040

uuaauguguu uagcacuugg aggaguauua auauuccuga gcacagcagu aagugcggac     2100


<210>  2548
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2548
cgaauaauaa acgccagaaa agaaaaaaag agaagagggg cagauacauc uguggguauc       60

guaggauuau uacuuacaac cgcuauggcg gcggaaguaa caagaagagg aucagccuac      120

uauauguacc uagacagaaa ugaugcuggu gaagcaauau cuuucccuac aacuuuagga      180

augaauaaau guuacaucca aauaauggac uuaggacaua ugugcgaugc aacuaugucg      240

uaugaauguc cuauguuaga ugaaggagua gaaccggaug acguugauug cugguguaac      300

acaacaagca ccuggguagu auacgggaca ugccaucaua aaaaagggga agcaagacga      360

agccgaagag cuguuacuuu accaagucau ucaacgagaa aacuucaaac gagaucucaa      420

acauggcuug aauccagaga auauacaaaa cauuuaauca ggguagaaaa cuggauuuuu      480

agaaauccag gauucgcauu agcagccgcg gcgauagcau ggcugcuagg gucaagcaca      540

ucucaaaaag uaauauaucu aguaaugaua cuauuaauag ccccagcaua uucaauaaga      600

uguauaggag uaaguaacag agauuuugua gaaggaaugu cugguggaac cuggguagau      660

auaguauuag aacauggagg auguguaacu gucauggcac aagauaaacc caccguagac      720

auagaacugg uaacaacaac aguaucaaau auggcugaag uacguaguua uugcuaugaa      780

gcaucaauau cagauauggc cucagauuca cgauguccua cccaaggaga agccuacuua      840

gauaaacaau ccgacacaca auacgugugu aaaagaacau uagucgauag aggcugggga      900

aauggcugug gauuauuugg aaaaggauca cuaguaacau gugcaaaauu cgcgugcuca      960

aaaaaaauga caggaaaauc cauucaaccu gaaaacuuag aauauagaau uauguuaagu     1020

guacacgggu cacaacauag cggaaugaua guaaacgaca caggacauga aacagacgag     1080

aacagagcca aaguagaaau aacaccaaau agcccaagag cugaagcaac acuaggugga     1140

uucggaaguu uaggauuaga cugugagcca cgaacugguu uagacuucag cgaucuguac     1200

uauuuaacaa ugaacaauaa acacuggcua guacauaaag aaugguucca ugauauacca     1260

uugccauggc augcaggagc agauacagga acaccacauu ggaauaauaa agaagcauua     1320

guagaauuua aagaugcaca cgcaaaaaga cagacgguag uaguuuuagg aucacaagaa     1380

ggagcugucc auacagcucu cgcgggcgca cuugaagcug aaauggacgg agcgaaaggc     1440

agacuaucau ccggucacuu aaaauguaga cuaaaaaugg auaaacuaag acuuaaagga     1500

guaucauauu cauuauguac ugcugcauuu acauuuacua aaauaccagc agaaacacuu     1560

cauggaacgg uaacuguaga aguacaauau gcugggacag auggaccaug caaaguacca     1620

gcacaaaugg caguugacau gcaaacauug acaccaguug gaagauuaau uacagcaaau     1680

ccuguaauaa cagaaagcac agagaauuca aaaaugaugu uagaauuaga uccaccguuc     1740

ggagauagcu auauaguaau uggaguagga gaaaaaaaaa uaacacauca uuggcacaga     1800

ucaggaucaa cuauaggcaa agcuuuugaa gcaacaguua ggggcgcaaa aaggauggca     1860

guauuaggag auacagcaug ggacuuugga agcguaggag gugccuuaaa uagucuugga     1920

aaaggaauac aucaaauuuu uggagcagca uuuaaaaguc uauuuggugg aaugaguugg     1980

uuuagucaaa uucuaauagg aacucuauua auguggcuag gacuaaauac aaaaaacggg     2040

ucaauaucau ugaugugucu ugcauuagga ggagugcuaa uauucuuaag uacagccgua     2100

agcgcu                                                                2106


<210>  2549
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2549
auaaacgcaa gaaaggaaaa aaaaagaaga ggugccgaua caucaguagg aauuguuggu       60

uuguuguuaa caacagcaau ggcagcagaa guuacgagaa gaggaucagc auacuacaug      120

uaucuugacc gaaacgaugc aggcgaagca auaagcuuuc ccacaacauu aggaaugaau      180

aaauguuaua uucagauaau ggauuuagga cauaugugcg augcuacgau gaguuaugaa      240

ugucccaugc uagaugaagg aguugaacca gaugaugugg auuguuggug uaauacaaca      300

ucuacguggg uaguauacgg aacaugccau cauaaaaaag gcgaagcaag aagaucaaga      360

cgagcgguaa cauuaccuuc acauucaacu agaaaacucc aaacgagaag ccagacaugg      420

cuggaaucaa gagaauauac aaaacaucuu auuagaguag aaaacuggau auuucgaaau      480

ccagguuucg cuuuagcagc agcagcaaua gcauggcuac uaggaucuuc uacaucacaa      540

aaaguaauau auuuaguaau gauuuuguua auagcaccag cauacaguau aagguguaua      600

gguguaagua auagagacuu uguagaagga augagcggag gaacgugggu agacauagua      660

uuagaacaug gaggaugugu uacaguaaug gcacaggaua aaccuaccgu agacauagaa      720

uuggucacaa cuacagucag caacauggcu gaaguacgaa guuauugcua ugaagcuuca      780

auuagugaua uggcaucaga uagcaggugu ccaacacaag gugaagcaua uuuagauaaa      840

caaagcgaua cacaauaugu auguaaaaga acauuaguag acagaggaug ggggaauggu      900

uguggacuau uugguaaagg aagcuuagua acaugcgcua aauuugcaug cucaaaaaaa      960

augacuggca aaagcauaca accagaaaau uuagaguauc gaauaauguu aucagugcac     1020

ggaagucaac acucaggaau gaucgugaau gacacaggcc augaaacgga ugaaaacaga     1080

gcaaaaguag aaauaacucc aaacucacca agagcagaag caacacuagg aggauuugga     1140

ucauuaggac uagacugcga accuagaaca ggacuagauu ucucagauuu auacuaucuu     1200

acaaugaaua acaaacauug guuaguccau aaagaauggu uucaugauau uccauuacca     1260

uggcaugcag gagcugauac aggaacgccc cauuggaaua auaaggaagc uuuaguagaa     1320

uuuaaagaug cacacgcaaa aagacaaaca guaguuguau uagggaguca agagggugca     1380

guacacacag cauuagcugg ugcgcuagaa gcagaaaugg acggggcaaa aggaagacuu     1440

aguucuggac auuuaaaaug cagacuaaaa auggauaaau uaagauuaaa aggcguaagu     1500

uauaguuuau gcacugcugc auucacauuu acaaaaauac cagcagaaac auuacaugga     1560

acgguaacag uagaaguaca auaugcagga acugauggac caugcaaagu gcccgcacaa     1620

auggcagugg auaugcaaac gcucacacca guaggaagau uaauaacagc uaauccaguc     1680

auaacagaau caacugaaaa uucaaagaug auguuagaau uagacccacc uuuuggagau     1740

ucauacauug uaauaggagu aggagaaaaa aaaauaacac aucauuggca uagaagugga     1800

ucaacaauag gaaaagcauu ugaagcaaca guacgagggg ccaaaagaau ggcaguacua     1860

ggagauacag caugggacuu uggaucggua gggggagcuc uaaauuccuu aggaaaaggc     1920

auacaucaaa uauuuggagc ggcguucaaa ucuuuauuug gcgguauguc uugguucucu     1980

caaauuuuaa uaggaacccu ucuaaugugg uuagguuuaa auacuaaaaa uggaucuauc     2040

agcuuaaugu gcuuagcccu aggaggugua uuaauuuuuu uaucaacagc aguuucagcu     2100


<210>  2550
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2550
auaauaaaug cuaggaaaga aaaaaaaaga cggggagcag auacaagugu aggaauagua       60

ggauuacuac uaacaaccgc cauggcagca gaagucacaa gaagagguuc agcauacuau      120

auguacuuag acaggaacga ugcaggagaa gccauuaguu uuccgacaac acuagguaug      180

aacaaauguu auauacaaau aauggauuua ggacauaugu gcgaugcaac aaugucauau      240

gaaugcccaa uguuagacga aggaguagaa ccagaugaug ucgauuguug gugcaauaca      300

acaucaacau ggguaguaua cgguacgugu caucacaaaa aaggcgaagc acguagaagu      360

cgaagagcag uaacucuacc aagccauagu acaagaaaac uacaaacucg aucacaaacg      420

ugguuagaaa gcagggaaua uacuaaacac uuaauaagag uagaaaacug gauauuucga      480

aauccagguu uugcuuuagc ugcagcagca auagcauggc uauuaggguc auccacguca      540

caaaaaguaa uauauuuagu aaugauauua cuaauagcac cagcguacuc cauaagaugu      600

auaggaguua gcaauagaga cuucguugaa ggaaugucug guggaacaug gguugauaua      660

guccuugaac acggaggaug uguaacgguc auggcacaag auaaaccaac uguugauaua      720

gaacuaguaa ccacaacagu aagcaauaug gcagaaguca gaucauauug uuacgaagcc      780

aguauaagcg auauggcauc agauucaaga uguccaacuc agggugaagc auauuuggau      840

aaacaaucag auacacaaua ugucugcaaa cgcaccuuag uagauagagg gugggguaau      900

gguuguggac uauuuggaaa aggaucacua guaacaugcg caaaauucgc auguucaaaa      960

aaaaugacag ggaaauccau acaaccagaa aauuuagaau acagaauaau gcuaucaguc     1020

cauggaucac aacauagugg uaugauuguu aaugauacag gucacgaaac agaugaaaau     1080

agagcaaaag uagaaauaac accaaauagu ccaagagcag aagcaacauu gggaggauuu     1140

ggaucucuag gucuagauug ugaaccuaga acaggacugg acuuuaguga ccuuuauuac     1200

uuaacaauga acaauaaaca uuggcuagua cauaaagagu gguuccauga uauaccacua     1260

ccauggcaug ccggagcaga uacaggaacu ccccacugga auaauaaaga agcucuagua     1320

gaauuuaaag acgcacaugc caaaagacaa acaguagucg uauuaggauc ccaagaagga     1380

gccguacaua cagcacuagc cggagcauua gaagcagaaa uggauggagc aaaaggacga     1440

cugagcucag gucaucuaaa auguagauua aaaauggaca aauuaagauu aaaaggggug     1500

ucauacaguc uauguacagc cgcauuuacu uucacaaaaa uaccagcuga aacauuacac     1560

ggcacaguga ccguugaagu acaauaugca ggaacagaug guccaugcaa aguacccgca     1620

caaauggcag ucgauaugca aacauuaaca ccaguaggaa gauuaaucac agcaaaucca     1680

guaauaacag aaaguaccga aaauucaaaa augauguugg aauuagaucc accauuugga     1740

gauaguuaua uaguaauagg aguuggagaa aaaaaaauca cccaccacug gcacagaagu     1800

ggcaguacaa uagguaaagc guucgaagca acugucagag gagcaaaaag aauggccgua     1860

cuuggagaca cagcauggga uuuuggauca guuggcggcg cucugaacuc ccuaggaaaa     1920

ggaauccauc aaauauuugg agcagcauuu aaaaguuuau uuggagguau gaguugguuc     1980

ucacaaauac uaauuggaac acuucuaaug uggcuaggac uaaauacaaa gaacggcagu     2040

auaucauuga uguguuuagc gcuaggaggc guacuaaucu uucuaagcac agcgguuuca     2100

gcu                                                                   2103


<210>  2551
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2551
aaugcaagaa aagaaaagaa aagaagaggu gccgauacau ccguagguau aguuggcuua       60

uuauuaacaa cagccauggc agcugaagug acuagaagag guucagcaua uuacauguau      120

cuagauagaa augacgcagg agaagcaaua aguuuuccca cuacauuagg aaugaauaaa      180

uguuauauac aaauaaugga ucuuggacac augugcgaug cuacaaugag uuaugaaugu      240

ccaauguuag augaaggagu ggaaccagau gauguagauu guugguguaa cacgacauca      300

acuuggguag uuuauggaac augucaucau aagaaaggag aagcaagacg aucaagacga      360

gcaguaacac ucccaagcca cucuacaaga aaauuacaga caagaucaca aacaugguua      420

gaaucccgag aauacacaaa acaccuaauu agaguagaga acuggauuuu uaggaaucca      480

ggauuugcau uagcggccgc ugcaauagca uggcuacuag gaucaaguac aagucaaaaa      540

gucaucuauu uaguaaugau auuauuaaua gccccugcau acucaauaag auguauuggc      600

guaaguaaua gagacuuugu agaagggaug aguggaggaa cuuggguaga uauaguauua      660

gagcauggag gauguguaac aguaauggca caagacaaac caacuguaga uauugaguua      720

guaacaacaa cugugaguaa cauggcagaa guaagauccu auugcuacga agcauccauu      780

ucagauaugg cuucagauag uagaugucca acucaaggag aagccuaucu agacaaacaa      840

agugacacac aauauguuug uaaaagaaca uuaguagaua gaggaugggg uaauggaugu      900

ggcuuauucg gaaaagguuc auuaguuaca ugcgcaaaau uugcuuguuc aaagaaaaug      960

accggcaaau caauucaacc cgaaaauuua gaauauagaa uaaugcucag uguacacgga     1020

agccaacauu cagguaugau aguaaacgau acugggcaug aaacagacga aaauagagcu     1080

aaaguagaaa uuacaccaaa uuccccaaga gcagaagcua cauugggagg auuugggagu     1140

cuaggacuug auugugaacc aagaacaggu cuagacuuca gugaucugua cuaucuuaca     1200

augaauaaca aacauuggcu uguccauaaa gaaugguuuc acgacauacc acuaccaugg     1260

caugcaggag cagauacugg aacgccacac uggaauaaca aagaagcauu aguugaauuu     1320

aaagaugcac augcgaaaag acaaacagua guaguuuuag guagccaaga aggagcugua     1380

cauacagcau uagcaggagc auuagaagca gaaauggaug gagcgaaagg uagauuauca     1440

ucaggacauc uaaaaugucg acuaaaaaug gauaaauuaa gacucaaagg aguuucauac     1500

ucauuaugua cagcagccuu uacuuucacc aaaauaccag cagaaacacu gcauggaacu     1560

guaacaguug aaguacaaua cgcaggaaca gauggaccau guaaaguacc agcgcagaug     1620

gcaguagaua ugcaaacacu cacaccugua gguagacuaa ucacagcaaa uccgguaaua     1680

acagaaagua cagaaaauuc uaaaaugaug cuagaacuag auccgccauu cggagacagc     1740

uacauaguaa uaggaguugg ugagaagaaa auaacacacc auuggcacag aucaggaagu     1800

acaauaggaa aagcauuuga agcaaccgua agaggagcca aaaggauggc agucuuagga     1860

gauacagcau gggauuuugg aaguguaggg ggugccuuaa auuccuuggg uaaagguaua     1920

caucaaauuu uuggagcagc auuuaaauca uuauuuggcg gcauguccug guucucacaa     1980

auacugauag gaacucuauu aauguggcuu ggcuuaaaua cgaaaaaugg aaguauauca     2040

uugaugugcc uagcauuagg cggaguucua auauuuuuaa guacagcagu aucagca        2097


<210>  2552
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2552
gcaagaaaag aaaagaaaag aagaggagcg gacacaagug ugggaauagu aggacuauua       60

uuaacaacag cuauggcagc agagguaacg aggagaggau cagcauauua uauguaucua      120

gaccgaaaug augcaggaga agcuauuuca uucccaacga cucuaggaau gaacaaaugc      180

uacauacaaa uaauggacuu aggacauaug ugugacgcca caaugaguua ugaaugccca      240

augcuagaug aaggcguaga accagaugac guagauuguu ggugcaauac aaccucgacc      300

uggguagucu acggaacaug ucaccauaag aaaggagaag caagaagguc aagacgagcc      360

gucacuuuac cuagccauuc aacacgaaaa cuacaaacaa gaucacaaac auggcuagaa      420

aguagagaau auaccaaaca uuuaauaaga guagaaaauu ggauauucag aaacccagga      480

uucgcauuag cugcagcagc uauagcuugg cuauuaggca gcucaacaag ucaaaaagug      540

auauaucugg uaaugauacu acuuauagcu ccugcauacu caauuagaug uauaggcgua      600

ucaaauagag auuuuguuga aggaaugagc ggaggaacau ggguagacau aguauuagaa      660

cacgguggau gugugacagu uauggcacaa gauaaaccaa caguagauau ugagcuaguu      720

acuacaacag uaucaaacau ggccgaagua agaaguuacu gcuacgaagc aucaauaagu      780

gauauggcau cagauagcag auguccaacc caaggugaag cauauuuaga uaaacaauca      840

gauacucaau auguuuguaa aagaaccuua guagauagag gauggggaaa uggauguggg      900

uuauuuggaa aaggaaguuu aguaacaugc gcuaaauuug ccuguucgaa gaaaaugaca      960

ggaaaaucua uucaaccaga aaaucuagaa uaccgaauaa ugcuuagcgu gcauggaagu     1020

caacacagcg gaaugauagu caacgauacu ggacaugaaa cggaugaaaa cagagcaaaa     1080

guugaaauaa caccgaauag ucccagagca gaggcaacau uaggaggauu ugguuccuua     1140

ggacuagacu gcgagccaag aacaggccua gauuucagcg auuuauauua ucuaacuaug     1200

aacaauaaac acugguuggu ccauaaagaa ugguuucaug auauacccuu accuuggcau     1260

gcuggugcag auacaggaac accacauugg aauaauaaag aagcacuugu agaauuuaaa     1320

gaugcacaug caaaaagaca aacaguagua gucuuaggau cucaagaggg agcaguacac     1380

acggcacuug cuggggcauu agaagcagaa auggauggag cuaaaggucg auuaaguagu     1440

ggacacuuaa aaugccgauu aaaaauggac aaauuaagau uaaaaggagu aucauauuca     1500

cuaugcacag cugcuuucac auuuacaaaa auaccagcug aaacacuaca uggaacagua     1560

acaguagaag uacaauacgc aggaacagau ggaccgugua aaguaccagc acaaauggca     1620

guggauaugc aaacacuuac gccaguagga agauuaauaa cagcuaaucc cguaauuacu     1680

gaaaguacag aaaauucuaa aaugaugcua gaacuagacc caccuuucgg ugauaguuau     1740

auaguaauag gaguaggaga aaagaaaauc acacaucauu ggcaucgauc aggaaguaca     1800

auaggaaaag cauuugaagc uacaguaaga ggcgcuaaac gaauggcagu ucugggagau     1860

acugcauggg auuuuggaag uguaggagga gcacuaaacu cauuaggaaa aggcauccac     1920

caaauauuug gagcugcauu uaaaagucua uucggaggua ugucaugguu cagucaaaua     1980

uuaauaggaa cguuauugau gugguuaggc uuaaauacua aaaacgguuc aauuucacua     2040

augugucuag cacuaggagg aguauuaaua uucuuaagua cggcaguaag ugca           2094


<210>  2553
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2553
cggaaagaaa aaaaaagaag aggugcugau acaaguguag gaaucguagg acuauuauua       60

acaacagcca uggcagccga aguaacaagg agaggcuccg cauauuauau guaucuagau      120

cgaaacgaug cuggugaagc aauaucuuuu ccaacaacac uaggaaugaa uaaauguuau      180

auacaaauua uggaucuagg gcauaugugc gaugcaacua ugaguuauga augcccaaug      240

uuagacgagg guguagaacc cgaugauguu gauuguuggu guaauacaac aagcacaugg      300

guaguauaug gaacaugcca ccacaaaaaa ggugaggcaa gaagaagccg aagagcugua      360

acauuaccau cacauagcac uagaaaauua caaacgagaa gccaaacaug guuagaauca      420

agagaguaca cuaagcaucu aauuagagua gaaaacugga uauuuagaaa uccaggauuc      480

gcacuugcag cagcggcaau agcauggcua uuaggaucau caacaaguca aaaaguaaua      540

uauuuaguua ugauacuuuu aauagcaccc gcauauagca ucagaugcau uggaguauca      600

aauagagacu uugucgaagg uaugucugga gguaccuggg uagauauagu auuagaacau      660

gguggaugug uaacgguaau ggcacaagac aaaccaacug uggauauaga acuaguaacu      720

acaacaguaa guaauauggc agagguaaga ucuuacugcu augaagcauc aauaucagac      780

auggcaagcg acucaagaug cccgacacaa ggagaagccu auuuagauaa acaaucggac      840

acgcaauaug uuuguaaacg cacuuuagua gauagaggau gggguaaugg augugguuua      900

uuuggaaaag gaagcuuagu uacaugugcu aaauucgcau guucaaaaaa aaugacagga      960

aaaucaauac aacccgaaaa cuuggaauau cgaauaaugu uaagcguaca ugguucacaa     1020

cacucaggua ugauugucaa ugacacaggu caugaaacag acgaaaacag agcaaaagua     1080

gaaauaacgc caaacucacc uagagcugaa gcaacacuag gugguuuugg aucucuggga     1140

cuagauugcg aaccaagaac aggacucgau uucagugauu uauauuacuu aacuaugaau     1200

aauaaacacu ggcuagucca caaagaaugg uuucaugaua uaccacugcc auggcacgca     1260

ggugcagaua ccgguacacc ccauuggaau aacaaagaag cacuagugga auuuaaagau     1320

gcacaugcca aacgucaaac agugguaguu uuaggaaguc aagaaggagc aguccauacg     1380

gcucuagcag gugcacuaga ggcagaaaug gauggggcua aaggaagauu aucaagugga     1440

cauuuaaagu guagacuaaa aauggauaaa uugagauuaa aagggguaag uuacucauua     1500

ugcacagcug cauuuacauu cacaaaaauu ccagcagaaa cacuacaugg cacaguaaca     1560

guagaagucc aauacgcagg aaccgauggu ccguguaagg uaccugcaca aauggcagua     1620

gacaugcaaa cauuaacacc agucggccga cuaauaaccg caaauccagu aauuacagaa     1680

aguacagaaa auaguaaaau gauguuagaa uuagauccac cauuuggaga uuccuacauu     1740

guuauaggag uaggugagaa aaaaauaacg caucauuggc acagaagugg aaguacuaua     1800

ggcaaagccu uugaagcuac aguaagaggg gcgaaaagaa uggcaguauu aggagauaca     1860

gcuugggauu uuggaagugu cggcggcgca cuuaauaguu uaggcaaagg uauucaccaa     1920

auauuuggag cggcauucaa aucacuauuc ggaggaaugu cuugguuuuc ucaaauauua     1980

auaggaacau uacuaaugug guuaggacua aauaccaaaa auggcuccau aucacucaug     2040

uguuuagcau uaggaggggu acuaauauuc uuaagcacag caguaucagc a              2091


<210>  2554
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2554
aaagaaaaaa aaagaagagg agcagauaca aguguaggua uaguaggauu guuacuaaca       60

acagcuaugg cagcagaagu aacaagaaga ggaagugcau auuauaugua ccuagauaga      120

aaugaugcag gugaagcaau aaguuuucca acaacauugg gaaugaauaa auguuauaua      180

caaauaaugg auuuaggaca uaugugugac gcuaccaugu cauacgaaug uccaaugcua      240

gaugaaggag uagaaccaga ugauguagac uguuggugua acacuacuuc aacaugggua      300

guauauggaa caugccauca uaaaaaagga gaagcaagaa gaucaagaag agcaguaaca      360

cuaccaucac auucuacaag aaaauuacaa acacgaagcc aaacauggcu ugaaucacga      420

gaauauacaa aacaucuaau acguguagaa aacuggauau uuagaaaccc gggauucgcu      480

uuagcagccg cugcaauagc auggcugcua gguucaucaa ccucacaaaa agucauauau      540

cuaguuauga uacuacuaau agcucccgca uacucaauca gauguauagg aguaagcaau      600

agagauuuug uugaaggaau gucaggaggu acauggguag auauaguguu agaacaugga      660

ggauguguaa caguaauggc gcaagauaaa ccaacaguag auauagaauu aguaacaacu      720

acaguaagua acauggcuga gguuagaagu uacuguuacg aggcgucuau aagcgauaug      780

gcaucagaca guagaugucc aacccaaggu gaagcauauu uagauaagca gagugacaca      840

caauauguau gcaaaagaac guuaguugau cgaggauggg gaaauggaug uggauuauuu      900

gguaaaggaa gucuaguaac augugccaaa uuugcaugua guaaaaaaau gacaggaaaa      960

agcauacaac cugaaaaucu agaauauagg auaauguuaa guguacaugg aagccaacac     1020

ucuggcauga uuguaaauga cacaggucau gaaacggacg agaacagagc gaaaguagaa     1080

auaaccccga auagccccag agcugaagca acauuagggg gauuugguag ucuagguuua     1140

gauugugaac caagaacagg guuagauuuu agugauuuau auuacuugac aaugaacaac     1200

aaacauuggc uaguacacaa agaaugguuu cacgauauac cacuacccug gcaugcugga     1260

gcggacacag guacacccca cuggaauaau aaagaagcuc uagucgaauu uaaagaugca     1320

caugcaaaaa gacaaacagu aguaguuuua ggaucacaag aaggagcagu acauacggca     1380

cuagcaggag cguuagaagc agaaauggau ggggcuaaag ggcgauuauc aucaggacac     1440

uuaaaauguc gauuaaaaau ggauaaacua agauuaaaag gaguaucaua uaguuuaugc     1500

acagcagcau uuacuuuuac aaaaauacca gccgaaacac uucaugguac uguaacagug     1560

gaaguccagu augcuggaac cgauggacca uguaaagugc cagcgcaaau ggcaguagau     1620

augcaaacau uaaccccggu aggcagacuu auaacugcca acccuguaau aacagaauca     1680

acggaaaacu caaaaaugau gcuugaauua gauccgccau uuggagacag cuacauugua     1740

auagguguag gagaaaaaaa aauaacacac cacuggcaua ggucaggauc gaccauuggu     1800

aaagcguuug aagcaacagu acgaggagcu aaaagaaugg caguacuugg agacacagcu     1860

ugggauuuug guucuguagg aggugcauua aacagccuag gaaaaggaau acaucaaaua     1920

uuuggagcag cuuuuaaaag ucuauuuggu ggaaugaguu gguuuagcca aauauuaauu     1980

ggaacguuau uaaugugguu aggguuaaau acaaagaacg guaguauuag ucuuaugugc     2040

uuagcuuuag gaggaguacu aauauuuuua agcacagccg uaucagca                  2088


<210>  2555
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2555
gaaaagaaaa gaagaggggc ggauacaucc guaggaauag uuggacuauu auuaacaacu       60

gcaauggccg cagaaguaac acguagagga ucagcuuauu auauguaucu agauagaaac      120

gaugcaggag aagcuauaag uuuuccaaca acacuaggaa ugaauaaaug uuauauacaa      180

auaauggacc ucggccacau gugugacgcu acaaugucuu augaaugucc cauguuagau      240

gaaggagucg aaccagauga cguagauugc ugguguaaua caacaucuac uuggguagua      300

uaugguacau gucaucacaa gaaaggugaa gcaagaagau cacgaagggc aguaacuuua      360

ccaucucaca gcacuagaaa auuacaaacu agaagccaaa caugguuaga aucuagagaa      420

uauacaaaac auuuaauacg aguagaaaau uggauauuua gaaacccugg uuuugccuug      480

gcagccgccg cuauagcaug guuacuagga ucaucaacaa gccaaaaagu aauauaccua      540

guuaugauac uacuaauugc accagcauau ucaauuaggu guauaggagu uagcaauaga      600

gauuuuguag aagguauguc agguggaaca uggguagaca uuguauuaga acacggaggu      660

ugugugacug uaauggcgca agauaaacca acaguagaca uagaauuagu aacuacaacu      720

guaaguaaca uggcagaggu aagaucguau ugcuaugaag caaguauaag ugacauggcu      780

ucagauucaa gauguccaac acaaggugag gcauaucuag acaaacaauc agacacacaa      840

uacguaugua aaagaacuuu aguagaccgc ggcuggggaa acggaugcgg acuauuuggu      900

aaaggaaguu uaguaacaug cgcaaaauuc gcauguucua agaaaaugac aggaaaauca      960

auacaaccug aaaaucuaga auauagaaua augcuaagug uacauggaag ucaacauuca     1020

ggaaugauug ugaacgacac uggacaugaa accgaugaaa auagagcaaa aguagaaaua     1080

acaccaaauu cgccaagagc agaagcaacu cuuggggguu uuggaucauu gggauuagau     1140

ugcgagccua gaacaggauu agauuucagc gaucuuuauu acuugacaau gaauaauaaa     1200

cauugguuag uacauaaaga augguuccau gauauaccac uaccauggca ugcgggagca     1260

gauacuggua caccucauug gaacaauaaa gaagcacugg uagaguucaa agaugcccau     1320

gcaaaacgac aaacaguagu aguauuaggg ucacaagaag gagccguuca cacagcacuc     1380

gcuggcgcau uagaagccga aauggauggc gcaaaaggaa gauugucuuc aggacaucua     1440

aaaugccgau uaaaaaugga caaauuaaga cuaaaagggg uaagcuauuc auuauguaca     1500

gcggcauuua cauuuacaaa aauaccugca gaaacucuac auggaacagu aacaguggaa     1560

guacaauaug cagguacuga cgguccuugu aaaguaccag cacaaauggc aguagacaug     1620

caaacuuuaa caccaguagg gagauuaauu acugcgaauc caguuauaac agaaaguaca     1680

gaaaauucaa aaaugauguu agaauuagau ccaccauucg gagauagcua uauaguaaua     1740

gguguaggag aaaagaaaau aacucaucac uggcacagaa guggauccac aauaggaaaa     1800

gcauucgaag caacaguaag aggcgcaaaa agaauggcag uauuaggcga cacugcaugg     1860

gauuuugggu caguaggagg agcauugaau agucucggua aagguaucca ucaaauauuu     1920

ggagccgccu uuaaaaguuu auucggagga augucuuggu uuucucaaau cuuaauaggu     1980

acacuacuaa uguggcucgg acuaaauacg aaaaauggcu cgauaucacu aauguguuua     2040

gcucuaggag guguacuaau auuuuuauca acagcaguga gcgcc                     2085


<210>  2556
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2556
aagaaaagaa ggggagcaga uacaagugua ggaauaguag gacuauuacu gacaacagca       60

auggccgcgg aaguaacaag aagaggauca gcuuacuaca uguacuuaga ccgaaaugau      120

gcaggagaag caauaucguu uccaaccaca uuaggaauga auaaauguua uauacaaaua      180

auggauuuag gacauaugug ugacgcaaca augagcuaug aaugcccaau gcuagaugaa      240

ggagucgaac cugaugaugu agauuguugg ugcaacacaa cuagcacuug gguaguauac      300

ggaacauguc accauaagaa aggugaagcu agacgaagua ggagagcugu aacauuaccc      360

agucauagca caagaaaauu acagacaaga agccaaacau gguuagaauc aagagaauac      420

acaaaacacc uaauaagagu agaaaauugg auauuuagaa auccaggcuu cgcucuggca      480

gcagcagcga uagccuggcu acuaggaagu ucgacaaguc aaaaaguaau auauuuaguu      540

augauuuuac ugauugcucc agcauauagu auaagaugua uaggagucag uaaccgagau      600

uuuguagaag gcaugucagg aggaacaugg guagauauug uauuagaaca uggagguugu      660

gucacaguaa uggcacaaga caaaccaaca guagauauag aauuaguaac aacuacagua      720

aguaauaugg cagagguaag aucauauugu uaugaagcau ccauaaguga uauggcauca      780

gauucaagau guccuacuca gggagaagca uauuuagaua aacaaaguga uacacaauau      840

guauguaaaa ggacauuagu agaccgugga uggggaaaug gauguggacu auuuggaaaa      900

ggaucauuag uuacaugcgc aaaauuugca uguucuaaga aaaugacagg uaaaagcaua      960

caaccagaaa auuuagaaua ucgcauuaug cuaucaguuc auggauccca acauucaggu     1020

augaucguga acgauacagg gcaugaaaca gaugaaaaua gagcaaaagu agaaauaaca     1080

ccaaacagcc caagagcaga agcaacuuua ggcggcuuug gaaguuuagg auuagacugu     1140

gaaccaagaa cagguuuaga uuuuucagau cuauacuauu uaacaaugaa caauaaacau     1200

ugguuagucc auaaagaaug guuucacgac auacccuuac cauggcacgc gggugcagau     1260

acagguacuc cacauuggaa caacaaagaa gcauuaguag aauuuaagga cgcucaugca     1320

aaaaggcaaa cugugguagu auuaggauca caagagggag cuguucacac agcauuagca     1380

ggagcucuag aagcagagau ggauggagca aaaggaagau uaaguucggg acaucuaaaa     1440

ugucgacuaa aaauggacaa gcuaagauua aaaggaguaa guuauagucu augcacagca     1500

gcuuuuacau uuaccaaaau accagcugaa acgcuacaug ggacaguaac aguagaagua     1560

caauaugcag guacagaugg gccuuguaaa guaccagcac aaauggcagu agauaugcaa     1620

acacuaacac caguaggacg auuaauaaca gcaaauccag ugauuacuga aagcacagaa     1680

aacaguaaaa ugaugcuaga auuagaccca cccuuuggag acaguuauau aguaauaggc     1740

guaggagaaa agaaaauaac ucaucacugg cauagaagug gaagcacaau aggaaaagca     1800

uuugaagcaa caguuagagg agcaaaacga auggcugugc uaggagauac agcuugggau     1860

uuuggcucag uaggaggggc auuaaauuca uuagguaaag gaauacauca aauauucggu     1920

gcagcauuua aaagucuauu ugguggaaug aguugguuuu cacaaauauu aauagguacu     1980

cuacuaaugu gguugggauu aaacacgaaa aacggaucaa ucagucuaau gugccuagcg     2040

cuugggggcg uguuaauauu uuuaaguaca gcaguaagug ca                        2082


<210>  2557
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2557
aaaagacgug gagcugauac aagugucggc auuguagguc uguuauuaac aacagcaaug       60

gccgcagaag uaacaagaag aggaucagca uauuauaugu auuuagauag aaaugaugcc      120

ggagaagcaa uaucuuuucc uacaacauua gggaugaaua aaugcuauau ucaaauaaug      180

gauuuagggc acauguguga ugcaacgaug aguuacgaau gcccaauguu agaugaagga      240

guagaaccgg acgauguaga uuguuggugu aacacuacau caacaugggu uguuuacgga      300

acaugucauc auaagaaagg agaagcaagg agaagucgca gagcagucac auuaccuagu      360

cacucaacaa gaaaauuaca aacaagaagu caaacauggu uagaaaguag agaauauacc      420

aaacaucuaa uaagagucga aaauuggauu uuuagaaacc cgggauucgc acuggcagca      480

gcugccauug cauggcuccu gggaucaagu acgucacaaa aaguaauaua uuuaguaaug      540

aucuuauuaa ucgcaccggc auacucaauu agauguauag gaguaagcaa cagagauuuu      600

guagaaggga ugucaggugg aacaugggua gauaucguau uagaacaugg cggguguguc      660

acaguaaugg cacaagauaa accuacaguu gauauagaau uagugacaac cacaguuagu      720

aauauggcag aaguacgauc auauuguuau gaggcuucca uaucagacau ggcaagugac      780

ucacgaugcc caacucaagg agaagccuau uuagacaaac aaucugauac acaauacgua      840

ugcaaaagga ccuuagucga cagagguugg ggaaauggau gcggacuauu ugggaaaggu      900

agcuuaguaa caugugcaaa auuugcaugc ucgaagaaaa ugacaggaaa aagcauacaa      960

ccagaaaauu uagaauauag aauaauguua agcguucaug gaucacaaca caguggaaug     1020

auaguaaaug auacagggca ugaaacagac gaaaauagag cuaaaguaga aauaacucca     1080

aacagcccaa gagcggaagc aacauuagga ggauuuggaa guuuaggacu agauugcgaa     1140

cccagaacag gacuggacuu uagcgaccua uauuauuuaa caaugaauaa uaaacauugg     1200

cugguucaua aagaaugguu ucaugauauu ccuuuaccgu ggcaugccgg ugcagauaca     1260

gguacaccac auuggaauaa caaagaagca cucguagaau uuaaagaugc acaugcaaaa     1320

agacaaacag uaguagucuu aggaucccaa gaaggagcag uacacacugc ucuagcggga     1380

gcccuagaag cagaaaugga cggcgcuaaa ggaagauuau cuucaggaca ucuaaaaugu     1440

agacuaaaaa uggauaagcu aagauuaaaa ggcguaucuu auagccuaug uacugcagcu     1500

uuuacauuua caaaaauacc agcagaaacu uuacauggaa caguaacagu agaaguacaa     1560

uacgcaggaa cugacggacc augcaaaguu cccgcacaaa uggcagucga caugcagaca     1620

cuaacaccag uuggaagauu aauaacagca aauccaguaa uaacagaauc aacagaaaau     1680

ucuaaaauga ugcuugaauu agacccgccc uucggagauu cauauauagu aauuggugua     1740

ggagaaaaga aaauaaccca ucacuggcac agaaguggau ccacaauagg uaaagcauuu     1800

gaagcaacag uaagaggugc caaaaggaug gcaguccuag gagauacagc cugggauuuu     1860

ggaaguguug guggggcacu uaacaguuua ggaaaaggaa uacaucaaau uuuuggagca     1920

gcauucaaaa gucuauucgg aggaaugagu ugguuuucac aaauacuaau cggaacacua     1980

uuaauguggu uaggauuaaa uacuaaaaac ggaagcauau cauuaaugug ucuugcauua     2040

ggcggagugc uaaucuuuuu aagcacagca guaagcgca                            2079


<210>  2558
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2558
agaaggggug cggauacauc uguaggaaua guaggauuau uauuaacaac ggcaauggca       60

gcagaaguua caagaagagg aagugcuuac uacauguauu uagauagaaa cgaugcuggu      120

gaagccauaa guuucccaac uacauuagga augaacaaau gcuacaucca aaucauggac      180

cuuggacaua ugugugaugc uacaauguca uacgaauguc caaugcugga ugaaggugua      240

gaacccgaug auguagacug uugguguaac acaacaucua cuuggguugu uuaugguacg      300

ugucaccaua agaaagguga agcaagacga ucaagacgag caguaacauu acccagucac      360

ucaaccagaa aacuacaaac aagaucacaa acaugguuag aaucaagaga auauacaaaa      420

caucuuauaa gaguagaaaa cuggaucuuu agaaacccug gauuugcauu agcagcagca      480

gcuauagcau gguuacuagg uucgaguaca agucaaaaag uuauuuaucu cguaaugaua      540

cuauuaauag caccagcuua uagcauaaga uguauaggug uaucaaauag agauuuugua      600

gaagggaugu caggagggac auggguagau aucguauuag aacauggagg auguguaaca      660

gucauggcgc aagacaaacc aacaguugau auagaauuag uaacaacaac aguuuccaau      720

auggcagaag uuagaucaua uuguuaugaa gcuagcaucu cagauauggc uuccgacuca      780

agauguccaa cacaaggaga agcauauuua gacaaacaau cugauacaca auauguaugu      840

aaaagaacuc uaguagauag agguugggga aaugguugug gauuauuugg caaaggaucu      900

uuaguuacau gcgcaaaauu ugcauguagu aagaaaauga caggaaaaag cauucaaccu      960

gaaaauuuag aauauagaau aaugcuauca guacacggaa gucaacauag cgguaugaua     1020

guaaacgaua caggacauga aacagaugaa aacagagcaa aaguggaaau aacaccaaac     1080

ucaccuagag cagaagcaac auuaggcgga uuugguaguu uaggauuaga cugugaacca     1140

agaacagggu uagauuuuag ugauuuguau uauuuaacaa ugaauaacaa acauuggcua     1200

guacauaaag aaugguucca cgauauacca uuaccguggc augcaggggc agauacugga     1260

acuccccauu ggaacaauaa agaagccuug guagaauuua aagacgcaca ugcaaaacga     1320

caaacagucg uaguauuagg cucacaagaa ggcgcaguac auaccgcuuu agcaggcgcu     1380

uuagaagcag aaauggaugg agccaaagga aggcuaucau cuggucaucu uaaauguaga     1440

cuaaaaaugg auaaacuaag acuaaaagga guuucuuaca gucucuguac agcagcauuu     1500

acauucacga aaauaccagc ugaaacacua cauggaacug uaacaguaga aguacaauau     1560

gcaggaaccg auggaccaug uaaaguacca gcacaaaugg caguugauau gcaaacauua     1620

accccaguag ggagacuaau aaccgcaaau ccaguuauua cagaaucaac agaaaacagu     1680

aaaaugaugc uagaauuaga ucccccuuuu ggcgauucau acaucguaau agguguggga     1740

gaaaagaaaa uaacgcauca cuggcaucga ucaggaagca caauaggaaa agcauuugaa     1800

gcuacaguca gaggagcuaa aagaauggcc guucuaggag acacugcuug ggacuucgga     1860

ucaguuggug gagcauuaaa uucucuggga aaaggaauac aucaaauauu uggugcagcc     1920

uucaaaucac uauuuggugg caugaguugg uucucacaga uacuaauagg uaccuuauua     1980

auguggcuag gauuaaauac uaaaaacgga ucaauaucau ugaugugcuu agcccuagga     2040

ggaguuuuaa uauuucuaag cacagcuguc agugca                               2076


<210>  2559
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2559
cgaggagccg auacaagcgu uggaauaguu gguuuauuau uaacaacagc uauggcagca       60

gaaguaacca gacguggauc ggcauauuau auguaccuag acaggaauga ugcaggagaa      120

gcaauaucuu uucccacaac uuuaggaaug aauaaaugcu auauacaaau aauggauuua      180

ggacauaugu gugaugcaac uaugagcuau gaaugcccua uguuggauga agggguugaa      240

ccagaugaug uagauuguug gugcaacacu acaucaaccu ggguuguaua uggaacaugu      300

caucacaaga aaggagaagc ucgcagaagc agacgagcag uaacacuacc uucacauagc      360

acaagaaaac uacaaacacg aucucaaaca ugguuagaaa gcagagaaua cacaaaacac      420

cuaauaaggg uagaaaauug gauauuuaga aacccagggu uugcauuagc agcagcggcu      480

auagccuggc uauuaggauc uaguacauca caaaaaguaa ucuacuuagu gaugauauua      540

uuaauugcac cagcauacuc aauaagaugu auaggaguua gcaauagaga uuucgucgaa      600

ggaaugucag gcggaacaug gguagauauu guauuggaac auggaggaug cguaacagua      660

auggcacaag auaaaccuac uguagacaua gaauuaguaa caacaacugu aagcaacaug      720

gcagaaguaa ggucauauug uuacgaagca aguauaucag auauggcaag ugauucgaga      780

uguccaacac aaggcgaagc auauuuagac aaacaaucag auacacaaua uguauguaaa      840

agaacccuag uagauagagg auggggaaau ggaugugguc uauuuggaaa aggaaguuua      900

guaacaugcg cuaaauucgc augcagcaag aaaaugacag gaaaaucuau acaaccagaa      960

aauuuagaau acaggauaau guuaucaguc cacggaaguc aacauucagg aaugauaguc     1020

aaugacacag gacaugaaac ugacgaaaau agagcaaaag uagaaauaac accaaacuca     1080

ccaagagcag aagcaacacu agguggcuuc gguucauuag gacuggacug ugaaccaaga     1140

acaggauuag acuucagcga uuuauacuac uuaacuauga auaacaaaca uuggcuagua     1200

cauaaagaau gguuccauga cauaccauua ccuuggcaug cuggugcaga uacuggaaca     1260

ccacauugga auaauaaaga agcgcuagug gaguucaaag augcacaugc uaaacgacaa     1320

acaguaguag uauuaggcuc acaagaagga gccguccaua cagcacuugc aggagcacuu     1380

gaagcagaaa uggauggagc aaaaggaaga uuaucgagug gccacuuaaa augucgacua     1440

aaaauggaua aacuaagauu aaaaggaguu ucauauaguu uauguacagc agcauuuaca     1500

uucaccaaaa uuccagcaga aacauuacau ggaaccguaa cuguggaagu acaauaugca     1560

ggaacagaug gacccuguaa aguaccagca caaauggcag ucgauaugca aaccuuaaca     1620

ccaguaggaa gauuaaucac cgcaaacccu gugauaacag aaucuacaga aaacaguaaa     1680

augaugcuag aauuggaucc uccauucgga gauuccuaua uaguaauagg aguaggagaa     1740

aagaaaauua cucaccacug gcacagauca gguagcacca uagguaaagc auuugaagcu     1800

acaguaagag gugcaaaaag gauggcugua uuaggcgaca cagcauggga uuucggauca     1860

guuggaggcg cuuuaaacuc acucggaaaa ggcauucauc aaauauuugg agcagcauuu     1920

aaaaguuuau uuggaggaau gucaugguuu ucacaaauuc uaauaggaac auuacuaaug     1980

uggcuggguc ucaauaccaa aaacggcagu aucagcuuga ugugucuagc auuaggaggc     2040

guacuaauau uccuaagcac agcaguauca gcu                                  2073


<210>  2560
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2560
ggcgcagaua caaguguagg aauuguaggg uuauuacuaa caacagcaau ggcagcagaa       60

guaacaagaa gagguagugc auauuauaug uaucuugaua gaaacgaugc aggagaagcu      120

auuucauuuc caacaacacu aggcaugaau aaauguuaua uacaaaucau ggauuuagga      180

cacaugugcg augcuacaau gaguuaugaa uguccaaugc uagacgaagg cguugaacca      240

gaugauguag auugcuggug uaacacaaca aguacuuggg uaguuuaugg caccugucau      300

cacaagaaag gagaagcgcg aagaaguaga cgagcaguaa cauuaccauc acauagcaca      360

agaaaauugc agacuagaag ucaaacaugg cuugaaucaa gagaauauac aaaacauuua      420

auacgaguug aaaacuggau auuccgaaau ccagguuuug cacuagcagc agcagcaaua      480

gcauggcuac ugggaucaag uacuagccaa aaaguaauau auuuaguaau gauauuacua      540

auagcccccg cguacaguau aaggugcaua gguguaucga auagggauuu uguugaagga      600

augagcggag ggacuugggu ggauauagua cuagaacaug gagguugugu uacaguaaug      660

gcacaagaua aaccaacagu ugacauagaa uuagugacaa caacuguaag caauauggcu      720

gaaguaagga gcuauugcua ugaagcaagu auaucagaua uggcaaguga uucaagaugu      780

ccaacacaag gugaagcaua cuuagauaaa caauccgaua cucaauaugu auguaaaaga      840

acucuaguag aucgaggaug gggaaacgga uguggauuau uuggaaaagg gaguuuagua      900

acaugcgcaa aauuugcaug uaguaagaaa augacaggaa aaucaauaca gccagaaaau      960

cuagaauauc ggauaaugcu aucaguacau gguagucaac auucaggaau gauaguaaac     1020

gauacagguc acgaaacaga cgaaaaucga gcaaaaguug aaauaacacc uaacagucca     1080

cgagcugaag caacauuagg cggauuugga ucccuaggau uagacuguga accgaggaca     1140

ggacuagauu uuucagaucu auauuauuua acaaugaaca auaaacauug gcuaguucau     1200

aaagaauggu uucaugauau accauuacca uggcaugcug gugcagacac aggaacaccu     1260

cauuggaaua auaaagaagc auuaguagaa uuuaaagacg cacacgcaaa aagacaaaca     1320

guuguaguau uaggaagcca agaaggagcu guacauacag cacuugcugg agcacuagaa     1380

gcugaaaugg auggugcuaa aggcagauua agcagugguc auuuaaaaug cagauugaaa     1440

auggauaaau uacgauuaaa aggaguauca uauuccuuau gcacagcagc auuuacauuu     1500

acaaaaauac cagcagaaac auuacaugga acaguaacag uagaagucca auaugcagga     1560

acagacggac caugcaaagu accagcacaa auggcaguug auaugcagac acuaacacca     1620

guaggaagau uaauaacugc aaacccggua auaaccgaaa guacagaaaa uucaaaaaug     1680

auguuggagc uggauccacc auuuggggac ucauacauag uaauuggcgu aggagaaaag     1740

aaaauuacac accacuggca ucgaagugga agcacaauag gaaaagcauu cgaagccaca     1800

guaaggggag caaaaagaau ggcaguauua ggugacacag caugggacuu cgguucagua     1860

ggaggugcuu uaaauaguuu agguaaagga auacaccaaa uauuuggagc agcauuuaaa     1920

ucauuauuug gaggaaugag uugguuuuca caaauucuga uaggaacguu acuaaugugg     1980

cuuggcuuaa auacgaaaaa uggaagcauu ucauuaaugu guuuagcgcu ugguggaguu     2040

uuaauauuuu uaucuacugc aguuagcgcg                                      2070


<210>  2561
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2561
gcagauacgu cugucggaau aguuggauua cuacuaacaa cugcaauggc cgcagaagug       60

acuagacgag guucggcgua cuacauguau cuagacagaa augaugcugg agaagccaua      120

ucauuuccaa cuacauuagg aaugaauaaa ugcuacauac aaauuaugga ccuaggacau      180

augugugacg caacaauguc auaugaaugu ccaauguuag augaaggggu agaaccagac      240

gacguagauu gcugguguaa cacaacaucu acaugggugg uuuacgguac augucaucau      300

aagaaaggug aagcaagacg cucaagacga gcaguaacac ugccaucaca uaguacaaga      360

aaacuacaaa caagaucaca aacauggcuc gaaucgagag aauacacaaa acaucuuaua      420

agaguagaaa auuggauauu cagaaauccc ggcuucgcac uagcugcagc agcaauagca      480

ugguuacuug gaaguucuac gagucaaaaa guaauauauc uaguaaugau acuucuaauc      540

gcgccugcau auagcauaag guguaucggg gucucaaaua gagacuuugu agaaggaaug      600

ucaggaggaa cauggguaga uauuguguua gaacauggug gaugugucac aguaauggca      660

caagacaaac ccacaguaga cauagaauua guuacuacca caguaucaaa cauggcagaa      720

guucgaucgu auuguuauga agcauccaua agugauaugg caucagacag uagaugcccu      780

acccaaggag aagcauauuu agauaaacaa agcgauacuc aauacguaug uaaaagaacc      840

cuaguagauc guggaugggg uaacggaugu gguuuauuug guaaaggcag uuuaguaacu      900

ugugcaaagu uugcauguuc aaagaaaaug acagguaaau cuauacaacc ggaaaaccua      960

gaauaucgaa uaaugcuaag uguacaugga ucucagcaua guggaaugau aguaaaugac     1020

acagggcaug aaacagauga aaacagagcg aaaguagaaa uaacuccaaa cagcccaaga     1080

gcagaagcaa cauuaggagg auuuggcagu cuaggacuag auugugaacc aagaacugga     1140

uuagauuuuu cagaucuuua cuacuuaacu augaacaaca aacauugguu aguacacaaa     1200

gaaugguuuc augacauacc gcuaccaugg cacgcuggug cagauaccgg cacaccucau     1260

uggaauaaua aagaagccuu aguagaauuu aaagacgcac augcaaaaag acaaacagua     1320

guaguacuag gaucacagga aggagcagua cauacagccc uagcuggagc auuagaagcg     1380

gaaauggacg gagcuaaagg aagauuguca agcggacacu uaaaaugucg acuaaaaaug     1440

gauaaacuaa gacuuaaagg aguaucauau agcuuaugca cagcagcauu uacauuuaca     1500

aaaauaccgg cagaaaccuu gcaugggacu guaacaguag aaguacaaua ugcagggaca     1560

gauggcccau guaaaguacc agcacaaaug gcaguagaua ugcaaacccu aacuccagua     1620

ggaagauuaa uaaccgcaaa uccaguaaua acugaaagca cggaaaacuc aaaaaugaug     1680

cuagaacuag auccaccauu cggagacagu uauauaguaa uaggcguagg agaaaagaaa     1740

auaacgcacc auuggcauag aagcggaagu acaaucggaa aagcauuuga agcaacagua     1800

agaggcgcaa aaagaauggc aguacuagga gauacagcau gggacuuugg aucgguagga     1860

ggggcauuaa acaguuuagg gaaaggaaua caucaaauau uuggagcagc auuuaaauca     1920

cuauucggag gaaugagcug guucucacaa auauuaauag ggacauuauu gauguggcua     1980

ggccuuaaca cuaaaaaugg aucaauaagu cuaauguguu uggcauuagg cggaguacua     2040

auauuuuuau caacagcagu aucagca                                         2067


<210>  2562
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2562
gauaccucag uuggaauagu aggacugcua uuaacaacag cuauggcugc cgaaguaaca       60

agaagaggaa gcgcauacua uauguacuua gauagaaaug augcgggaga agcaauaagu      120

uuuccaacca cacuaggaau gaauaaaugu uacauacaaa uaauggaucu agggcauaug      180

ugugaugcga caaugucaua ugaaugucca auguuggaug aaggcguaga acccgaugau      240

guagacuguu gguguaacac aacaagcaca uggguaguau auggaacaug ucaucacaag      300

aaaggagaag cacgaagaag uaggagagca guuacauuac caucacauag uacuagaaaa      360

uugcaaacua gaucacaaac augguuagaa agccgcgaau auacaaaaca uuugauaaga      420

guagaaaauu ggauuuuuag aaacccaggc uucgcacuag ccgcagcagc aaucgcaugg      480

cuucuaggaa gcucuaccuc acaaaaagua auauauuugg uaaugauauu auuaauagca      540

ccagcuuaua guauaagaug uaucggagua aguaauagag auuuuguaga aggaaugagu      600

ggaggaacau ggguagacau agucuuagaa cauggaggau guguuacagu aauggcgcaa      660

gauaaaccaa caguagauau agaauuagua accacaacag uaaguaauau ggcagaagua      720

agaaguuauu guuaugaagc aagcauuagu gauauggcau cagauagucg auguccaaca      780

cagggagaag cauacuugga caaacaaagu gacacucagu auguauguaa gagaacauug      840

guagauagag gaugggguaa ugguugugga uuauucggaa aaggaucguu ggucaccugu      900

gcaaaauuug cuuguagcaa gaaaaugacc ggaaaaucaa uucagccaga aaacuuagaa      960

uauagaauaa uguuaagcgu ucauggaagu caacauagcg gcaugauagu aaaugauaca     1020

ggacacgaaa cagacgaaaa cagagcaaaa guagaaauua caccaaauag uccaagagca     1080

gaagcaacau uaggagguuu uggaucucuu ggauuggauu gugaaccaag gaccggacug     1140

gauuuuucag accuauauua cuuaacaaug aauaauaaac auugguuagu acacaaagaa     1200

ugguuucacg acauuccauu accuuggcac gccggagcag auacugggac accgcauugg     1260

aauaauaaag aagcauuagu agaauuuaaa gacgcacacg caaaacgaca aacaguaguu     1320

guucuaggaa gucaagaggg ggcaguucau acagccuuag caggggcacu agaggcagaa     1380

auggauggag cuaaaggaag acuaaguagu ggacacuuaa aauguagauu aaaaauggac     1440

aaauuacguc ucaagggagu cuccuauagc uuguguacag cagcauuuac uuucacaaaa     1500

auaccagcag aaacuuuaca uggaacagua accguagaag uacaauaugc aggaacagau     1560

ggaccaugca aaguaccagc acaaauggca gucgacaugc aaacacuaac accaguaggg     1620

agacuaauca cagcaaaucc uguaauaaca gaaucaacug aaaauagcaa gaugauguua     1680

gaauuagauc caccauuugg agauucauac auaguaauug gaguagguga aaagaagaua     1740

acacaucacu ggcacagaag uggaucaacc auaggaaagg cuuuugaagc aaccguaaga     1800

ggugcaaaac gaauggcugu uuuaggagac acagcauggg acuuuggguc aguaggugga     1860

gcauuaaaua gucuaggaaa agguauucau caaauauuug gagcagcuuu uaaaaguuua     1920

uucggaggaa ugucaugguu cagccaaaua uugauaggaa cuuuauuaau guggcuagga     1980

uugaauacca aaaaugguag uauuagucua augugucuug cguuaggagg aguauuaaua     2040

uuuuuaagca cagcaguauc agca                                            2064


<210>  2563
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  2563
acaagcguag gaauaguugg auuauuacua acaacagcaa uggcagcuga aguaacuaga       60

agaggaagcg cauauuauau guaccuagau cgaaacgacg cuggagaagc aauaucuuuc      120

ccaacuacau uaggcaugaa uaaaugcuau auacaaauaa uggauuuagg acauaugugc      180

gaugcaacaa ugucauacga augucccaug uuagacgaag gaguagaacc agaugaugua      240

gauuguuggu gcaauacaac aucaacaugg gucgucuaug gcacauguca ucauaagaaa      300

ggugaagcac ggagaagucg cagagcaguu acacuaccgu cacauucaac aagaaaacua      360

caaacuagau cacaaaccug guuggaaagu agagaauaua caaaacaucu aauaagggua      420

gaaaauugga uauucagaaa ccccggauuu gcuuuagcug cagcagcaau agcauggcua      480

cuaggaagua gcacaucaca aaaaguaaua uaucuaguaa ugauacuacu aauagcgcca      540

gcguauagua uaagauguau aggaguaucu aaccgugauu uuguggaagg gaugucaggu      600

ggaacauggg uagauauugu auuagaacau ggaggaugug uaacagucau ggcgcaagac      660

aaaccaacug uagauauaga gcuaguaacc acaacuguau caaauauggc agaaguuaga      720

uccuauugcu acgaagcauc gauuagcgau auggcaagug auuccagaug uccaacccaa      780

ggagaggcuu acuuagauaa acaaagugau acacaauaug uaugcaaaag aacauuaguc      840

gauagaggau ggggaaaugg augcggguua uucggaaaag gaucuuuagu gacaugugca      900

aaauuugcuu guucuaagaa aaugacaggc aaaucaauac aaccagaaaa cuuagaauau      960

agaauuaugu uaucaguaca cggaucacaa cauagcggaa ugauugucaa ugauacagga     1020

caugaaacag augaaaauag agcaaaagua gaaauaacac cuaauucacc uagagcagaa     1080

gcaacacuag gaggauucgg aaguuuaggu uuggauugcg aaccacgaac uggucuagau     1140

uuuucagauu uauacuauuu gacaaugaau aauaaacauu gguugguaca uaaagaaugg     1200

uuucaugaca uaccacuacc uuggcaugca ggagcagaca cuggaacacc acacuggaac     1260

aauaaagaag cacuugucga guucaaagau gcacaugcua aaagacaaac aguuguugua     1320

uuaggaagcc aagaaggagc aguacauaca gcauuagcag gggcguuaga agcagaaaug     1380

gauggagcaa aaggcagacu uucuuccgga cauuuaaaau guagauuaaa aauggauaaa     1440

uuacgccuaa aaggugucuc auacucauua uguacagcag cauuuaccuu uaccaaaauu     1500

ccagcggaaa cccuucaugg aaccguaaca guugaaguuc aauaugcugg cacagacgga     1560

ccaugcaaag uaccugcaca aauggcagua gauaugcaaa cucuaacucc aguugggaga     1620

cuaauaacag ccaauccagu uaucacagaa agcacagaaa auucaaagau gaugcucgaa     1680

uuagauccgc cauucgguga cucuuauaua guuauaggag uaggugaaaa gaaaauaaca     1740

caucauuggc accguagcgg aagcacuauu ggaaaagcau uugaagcaac aguuagaggu     1800

gcaaaaagaa uggcaguacu aggagauacc gcaugggauu ucggaagugu cggcggagca     1860

cuaaauucau uagguaaagg uauacaucaa auauuuggag cagcuuuuaa aagccuauuu     1920

gguggaauga guugguucuc gcaaauauua auaggaaccc uuuuaaugug gcuaggacua     1980

aauacuaaaa auggaaguau aagccucaug ugucuagcac uugggggagu auuaauauuc     2040

uuauccacag cuguaucagc a                                               2061


<210>  2564
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2564
acgucaguag gaauugucgg auuauuauua acuacagcaa uggcagcagc uguaaccaga       60

aggggaucag ccuacuauau guauuuggac cgaaaugaug cuggugaagc aauaucauuu      120

ccaacuacau uagguaugaa uaaaugcuau auacaaauaa uggaucuugg acauaugugu      180

gaugcaacca ugaguuauga auguccuaug uuagaugaag gaguagaacc ugaugacgua      240

gauugcuggu guaacacaac uucaacaugg guaguauaug gaacaugcca ucacaagaaa      300

ggagaagcaa gacgaagccg gagagcagua acauuaccaa gucacucaac gagaaaauua      360

caaacaagau cacaaacaug gcuugaaagu agagaauaca cuaaacauuu aauaagaguc      420

gaaaacugga uauuuagaaa uccaggauuu gcacuagcag cugcagccau ugcaugguua      480

uuaggaucaa guacaucuca aaaagucauu uacuuaguaa ugauauuacu cauagcuccu      540

gcauauucaa ucagaugcau aggcguuuca aacagagauu ucguagaagg aaugucaggu      600

ggaacauggg uagauaucgu acuagaacac ggaggaugcg uuacaguaau ggcacaggau      660

aaaccaaccg uagacauaga auuaguaaca acuacuguaa guaauauggc cgaaguaaga      720

ucauauuguu augaagccag uauuagcgau auggccagcg auagcagaug uccaacgcaa      780

ggagaagcau acuuagauaa acaaagugau acucaauaug uauguaaacg aacauuagua      840

gaucgaggau gggguaacgg augcgguuua uuuggaaaag gaucauuagu aacaugcgca      900

aaauuugcau gcucuaagaa aaugacugga aaaucaauac aaccagaaaa ucuagaauac      960

agaauaaugu uaucgguaca uggaagccaa cauucaggaa ugauaguaaa cgacacugga     1020

caugaaaccg acgaaaauag agccaaagua gaaauaacac cuaauucccc aagagcagaa     1080

gcaacauuag gaggauucgg aagccuagga cuagauugug aaccaagaac uggucuugac     1140

uucucagacu uauacuaccu aaccaugaac aacaaacacu gguuaguaca uaaagaaugg     1200

uuccacgaca uaccauuacc uuggcacgcu ggagcagaca caggcacacc ccacuggaau     1260

aauaaagagg cuuuaguuga guucaaagau gcucacgcua aaagacaaac aguaguagua     1320

cuagguucac aagaaggugc aguacauaca gcuuuagcag gagcacucga agcagaaaug     1380

gauggagcaa aaggaagauu aucaagugga caucuaaagu gcaggcuaaa aauggauaag     1440

uuaaggcuaa aaggaguauc auauucucua ugcacagcag cauuuacauu cacaaaaaua     1500

ccagcagaaa ccuuacaugg caccguaaca guagaaguac aauaugcagg uacagauggc     1560

ccuuguaaag ucccagcaca aauggccgua gauaugcaaa cauuaacacc agugggcaga     1620

uuaauaacag caaaccccgu aauaacugaa aguacagaaa auaguaaaau gaugcuagaa     1680

uuagauccuc cauuuggcga uaguuauaua guaauaggag uaggagaaaa gaaaauaaca     1740

caucacuggc accgcagcgg aaguacuauu ggaaaagcau uugaagcaac uguaagagga     1800

gcaaaaagaa uggcuguucu aggagacaca gcuugggacu uugguucugu aggaggagca     1860

cuaaauaguu ugggaaaagg cauacaucaa auuuuuggcg cugcauucaa aucacuauuu     1920

ggaggaauga guugguuuuc acaaauucuc auaggaacau uauuaaugug gcuaggguua     1980

aacacgaaaa auggaaguau aucauuaaug uguuuagccu uagguggagu auuaaucuuc     2040

uuaucuacag caguaucugc u                                               2061


<210>  2565
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2565
cguggagcug auaccucagu ugguauagua ggcuuacuau uaacaacagc aauggcugcc       60

gcuguuacua gaagaggauc agcauauuau auguacuuag auagaaauga ugcaggagaa      120

gcaaucucau uucccacuac uuuaggcaug aauaaauguu acauucaaau aauggauuua      180

ggacauaugu gugaugcaac aaugucauac gaaugcccaa uguuagauga aggcguagag      240

cccgaugaug uagauuguug guguaauacu acaucuacuu ggguagugua uggaacaugu      300

caucacaaaa aaggagaagc aagacgaagc agacgagcag uaacauugcc aucucacuca      360

acaagaaaau uacaaacaag aucacaaaca uggcuagaau cuagagaaua cacaaaacau      420

uuaauaagag uagaaaauug gauauuuaga aauccaggau ucgcguuggc ggccgcagcg      480

auagcauggu uauuaggauc aaguacauca caaaaaguua ucuauuuagu aaugauccua      540

cuaauagcac cagcguauag uauuagaugu auaggcguau caaauagaga uuucguagag      600

gggaugagug guggaacaug gguagauaua guuuuagaac auggugggug uguaacggua      660

auggcucaag auaaaccaac cguagauaua gaguuaguca caacuacagu cagcaauaug      720

gccgaaguaa gaucauacug uuaugaagca ucaaucucag auauggccag ugacaguaga      780

uguccaacuc aaggagaagc uuaucucgac aaacaaagug auacacaaua uguauguaaa      840

aggacacuag uggauagagg uugggguaau gguuguggac ucuuuggcaa agguagucua      900

gucacgugcg cuaaauuugc uugcucaaaa aaaaugacgg gaaaaagcau acaaccugaa      960

aauuuagaau accgaauaau guuaucaguu cauggcaguc aacauagcgg gaugauaguu     1020

aacgauacag gacaugaaac agaugaaaau agagcaaaag uggaaauaac accuaacuca     1080

ccuagagcgg aagccacguu aggaggauuu ggaaguuuag gauuagauug cgaaccaagg     1140

acaggauuag auuucucaga uuuauauuau cuaacuauga auaacaaaca cugguuagua     1200

cauaaagagu gguuccacga uauuccacua ccauggcaug cuggggcaga cacuggaaca     1260

ccucauugga auaauaaaga agcauuagua gaauucaaag acgcacaugc uaaaagacaa     1320

acuguagugg uacuaggaag ucaagaagga gcaguacaua ccgcacuugc uggagcacua     1380

gaagcagaaa uggauggugc aaaaggaaga cuuucaagug gacaucuaaa auguagauua     1440

aaaauggaca aauuaagauu aaaaggagua ucauacucau uauguacagc agccuuuaca     1500

uucacaaaaa uaccugcaga aacacuucau ggaacaguaa caguagaagu acaauaugcu     1560

ggaacugaug gcccguguaa aguucccgcu caaauggcag uugauaugca aacauuaacc     1620

ccugucggga gauugauaac agcuaaucca guuauaacag aaucuacuga aaacucaaaa     1680

augauguuag aauuagaccc accauucgga gauagcuaua uaguaaucgg gguaggugaa     1740

aaaaaaauua cacaccauug gcacagaagu ggaucuacaa uaggaaaagc auuugaagcc     1800

acuguuagag gagcaaaacg aauggcagua cuaggugaca ccgcauggga uuuuggauca     1860

gucggcggug cauuaaacuc acuaggaaaa ggaauccauc aaauauuugg agcugcauuu     1920

aaaagccuau uugguggaau gucaugguuu agccaaauac uaauuggaac uuuauuaaug     1980

uggcuaggau uaaauacaaa aaauggauca auuagcuuaa uguguuuagc cuuaggagga     2040

guuuuaauau ucuuaagcac agcuguaagu gca                                  2073


<210>  2566
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2566
agaggacuau cuagcagaaa aagaagauca caugacguau uaaccguuca guucuuaaua       60

uuaggaaugu uauuaaugac aggaggagua acaagacgug gcuccgcgua uuauauguac      120

cuagauagaa augaugcagg agaagcaauu ucauuuccua caacuuuagg aaugaauaaa      180

ugcuauauac aaaucaugga ccuaggacau augugcgaug ccacaaugag cuaugaaugu      240

ccaauguuag augaaggcgu agaaccagau gacgucgauu gcugguguaa cacaacuuca      300

accuggguag uauauggaac cugucaucau aagaaaggag aagcaagacg aucaaggcga      360

gcaguaacau uaccaucuca cucaacaaga aaauuacaga caagaucaca aacauggcua      420

gaaaguagag aauauacaaa acauuuaaua agagucgaaa auuggaucuu ucguaauccu      480

gguuuugcac uagcugcagc ugcuauagcu ugguuauuag gcucaagcac aucacaaaaa      540

guuauuuacu uaguaaugau auuacuaaua gcaccagcuu acucaauaag auguauaggu      600

guuuccaaca gagauuuugu agaagguaug ucuggaggaa cuuggguuga uaucgucuug      660

gagcauggug gauguguaac gguaauggcu caagauaaac cuacaguaga uauagagcua      720

guuacgacaa caguuagcaa uauggcugaa guccgaaguu auugcuacga agcaagcaua      780

agugauaugg caagugauuc aagaugccca acacaaggag aagcauaucu agauaaacaa      840

agugacacac aauacguuug uaaaagaacu cuuguagaua gggguugggg caauggaugu      900

gguuuauucg gaaaaggaag uuuaguaaca ugugcuaaau uugcuuguuc uaagaaaaug      960

acuggcaaau caauucaacc agaaaauuua gaauacagaa uaaugcuauc aguucauggu     1020

ucacaacauu cuggaaugau aguaaaugau acaggacaug aaacagauga aaaucgugcu     1080

aaaguagaaa uuacaccaaa cucaccaaga gcagaagcaa cucuuggugg auuuggcuca     1140

uugggguugg auugcgaacc aagaacugga uuagauuuca gugauuugua uuauuuaacu     1200

augaauaaua aacacuggcu aguucauaaa gagugguucc acgacauacc auuaccaugg     1260

cacgcaggag cagauacagg aacaccacau uggaacaaua aagaagcacu cguugaauuu     1320

aaagaugcac augcaaaaag acaaaccgug guaguauugg gaucucaaga aggagcaguu     1380

cacacagcac uagcaggagc auuagaagcc gaaauggacg gagcaaaagg aagguuaucu     1440

ucagggcauc uaaaauguag auuaaaaaug gacaaacuaa gauuaaaagg gguaaguuau     1500

ucacuaugua cagcagcuuu uacauucaca aaaauaccug cagaaacacu gcacgggaca     1560

guaacaguag aaguacaaua ugcaggaaca gaugguccau guaagguacc cgcacaaaug     1620

gcaguugaca ugcaaacucu aacgccagua ggaagacuaa ucacagcuaa uccaguaaua     1680

acagaaucaa cugaaaacuc aaaaaugaug cuagagcucg auccaccuuu cggagauuca     1740

uacauuguga uagguguugg agaaaagaaa auuacacauc auuggcauag aucaggauca     1800

acuauaggaa aagcauuuga agccaccgua agaggagcaa aaagaauggc ugugcuagga     1860

gauacugccu gggauuuugg aaguguagga ggagcauuaa auucacuagg gaaaggaaua     1920

caucaaauau uuggagcagc auuuaaauca cuauuuggug guaugagcug guuuucacaa     1980

auucuaauag gaacauuauu aaugugguua ggucuaaaca cuaaaaacgg aagcauaucu     2040

uuaaugugcc uagcacuagg ugggguacuc auauuucuaa gcacugcugu gagugca        2097


<210>  2567
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2567
agaggguuau caagucgaaa aaggagauca caugauguuu uaacaguaca auuuuuaauu       60

uugggcaugc uacuuaugac uggagcagua acaaggagag gaagugccua uuauauguau      120

cuugauagaa augaugcagg agaagcaauu ucauuuccaa caacauuagg aaugaauaaa      180

uguuauauac aaauaaugga uuuaggacac augugugaug caacaauguc auacgaaugu      240

ccaaugcuag augaaggugu ggaaccugau gauguagauu gcugguguaa cacaacaagc      300

acuuggguag uauacggaac augucaccac aagaaagggg aagcaagaag aaguagaagg      360

gcaguuaccc uaccaagcca uucaacaaga aaauuacaaa caagaucaca aacauggcua      420

gaaaguagag aauacacaaa acacuuaaua agaguagaaa acuggaucuu uagaaaucca      480

ggauucgcac uagcagcugc ugcuauugcu uggcuauuag gaagcaguac uagccaaaag      540

guaauauauc uuguuaugau auuacuaaua gcaccagcau acucaauaag gugcauagga      600

guaucaaaua gagauuuugu agaaggaaug uccggaggua caugggugga uauuguacua      660

gaacauggcg gcugcgucac agugauggca caagacaaac caacaguaga uauagaauua      720

guuacaacaa cagucucgaa uauggcagaa guaagaaguu auuguuacga agcaagcaua      780

agugauaugg caagcgacag ucggugcccu acucagggag aagcauauuu agauaaacaa      840

ucagauaccc aauacguaug uaaacgaacu cuaguagaca gagggugggg aaauggaugu      900

ggacuauuug guaaaggauc acugguaaca ugcgcaaaau uugcaugcag uaagaaaaug      960

acaggcaaaa guauucaacc ugaaaauuug gaauauagaa uaaugcuuag cguacaugga     1020

ucacaacacu caggaaugau agugaaugau acaggacacg aaacagauga aaauagagca     1080

aaaguagaaa uaacaccuaa uaguccuaga gcggaagcaa cacuaggagg auucggauca     1140

uugggucuug acugugaacc acgaacuggg uuagauuuuu cagaucuaua uuauuuaaca     1200

augaauaaua aacauugguu aguacacaaa gagugguucc augauauacc auugccaugg     1260

caugcaggag cggauacugg aacaccgcau uggaacaaca aagaagcacu uguagaauuu     1320

aaagaugcac augcaaaaag gcaaacagua guaguacuug gaucucaaga aggagcagua     1380

cauacugcuu uagcaggagc auuagaagcu gaaauggaug gagcaaaagg uagacuaagc     1440

ucgggacacu uaaaaugcag acuaaagaug gauaaauuaa gauuaaaagg ugugucguac     1500

aguuuaugua cagcugcauu uaccuuuacu aaaauaccag cagaaacauu acauggcacu     1560

guaacaguug aaguacaaua ugcaggaacc gaugguccau gcaaaguacc agcucaaaug     1620

gcaguagaua ugcaaacgcu uacuccagua ggaagguuaa uaacagcuaa uccaguaaua     1680

acugaaucca ccgaaaauag caaaaugaug uuagaauuag auccaccauu uggggauuca     1740

uauauaguua uaggaguagg agaaaagaaa auaacucacc auuggcacag aucaggaagu     1800

acaauaggaa aagcguucga agcaacagua aggggagcaa aacgaauggc aguauuagga     1860

gacacagcau gggacuuugg uucaguaggc ggagcauuga auagcuuagg aaaaggaaua     1920

caccagauau uuggcgcagc uuucaagagc uuauuuggag gaaugucgug guuuagccaa     1980

auacuaauag gaacuuuauu gauguggcua ggacugaaca cuaaaaacgg aaguauuuca     2040

uuaauguguu uagcauuagg aggaguauua aucuuuuuau caacagcagu aagugcu        2097


<210>  2568
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long CDS opt

<400>  2568
cguauaauaa augcaagaaa agaaaagaaa agaagaggcg cagauacaag cguaggcaua       60

guuggguuac uguuaacaac agcuauggcu gcagaaguaa caagaagagg uagcgcuuau      120

uacauguauu uagacaggaa ugacgcugga gaagcaauua gcuuuccaac aacuuuagga      180

augaauaaau guuauauuca aauaauggau cuaggacaca ugugcgacgc aaccaugagu      240

uaugaauguc caaugcuaga cgaaggagua gaacccgaug auguugauug uugguguaau      300

acaacgucaa ccuggguugu auauggcaca ugucaucaua agaaaggaga agcaagaaga      360

aguaggagag caguaacacu accuucacau ucaacuagaa aacuacaaac ucguagccaa      420

acauggcuag aaucacgaga auauacaaaa caucugauaa gaguagaaaa uuggauuuuu      480

agaaauccug gauuugcucu agcugcagcc gcaauagcau ggcuacuagg aucuaguacu      540

agccaaaaag ugauauacuu aguaaugaua uuacuaauag cuccugccua caguauacgc      600

ugcauaggag uaagcaauag ggacuucgua gagggaaugu caggaggcac auggguagau      660

auaguacuag aacacggagg auguguaaca guaauggccc aggauaaacc aacuguagau      720

auugagcuag uaacuacaac uguaucaaac auggcugaag ucagaaguua cuguuacgaa      780

gcaaguauaa gugauauggc cucagauuca cgauguccaa cucaaggaga agccuaucua      840

gauaaacaau cagauacuca auauguaugu aaaagaacuu uaguagauag aggcugggga      900

aauggcugug gauuauuugg aaaaggcagc uuagucacau gcgcaaaauu ugccugcagc      960

aagaaaauga cgggaaaauc gauacaaccg gaaaauuuag aauauagaau aauguuauca     1020

guacacggga gccaacauuc aggaaugaua guaaaugaua caggacacga aacggaugaa     1080

aauagagcca aaguugaaau aacaccuaac aguccaagag cagaagcuac acuaggugga     1140

uucggcucau uaggauugga uugugaacca cgaacuggcu uagacuucuc ugacuuauac     1200

uacuuaacua ugaauaauaa acauuggcua guacauaaag aaugguucca ugacaucccu     1260

uuaccauggc augcaggagc agauacagga acaccucauu ggaauaauaa agaagcacuu     1320

guagaguuca aagaugcaca ugcuaaaaga caaacaguag uaguauuagg aucacaagaa     1380

ggugccguac acacugcgcu agcuggugca uuagaagcgg aaauggaugg agccaaagga     1440

agacuaucuu ccggacaucu uaaauguaga uuaaaaaugg auaaacuaag auuaaaagga     1500

guaagcuaua guuuaugcac agcagcuuuu acauuuacaa aaauaccggc agaaacauua     1560

cauggaacag uaacaguuga aguucaauau gcgggaacgg auggaccuug caaaguccca     1620

gcccaaaugg cuguggacau gcaaacauua acgccaguag gaagacucau uaccgcaaau     1680

ccaguaauaa cugaaucaac ggaaaacagu aaaaugaugc uagaacuaga cccuccauuu     1740

ggagacagcu auauaguaau aggaguagga gaaaagaaaa uaacacauca uuggcaucga     1800

ucuggaagua ccaucggaaa agcauuugaa gcgacaguac gaggagccaa aagaauggcc     1860

guauuaggcg auacagcuug ggauuucggu ucggucggag gcgcauuaaa uaguuuagga     1920

aaaggaauac aucaaauuuu cggagcagca uucaaaagcc uauuuggggg caugagcugg     1980

uuuucacaaa uacucaucgg uacuuuauua auguggcuag gacucaauac gaaaaauggu     2040

aguaucucau uaauguguuu agcauuaggc ggaguauuga uuuuucuuag uacagcagua     2100

agugccgacg uaggaugcuc aguagauuuc aguaaa                               2136


<210>  2569
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long CDS opt

<400>  2569
agaauuauaa acgcuagaaa agagaaaaag cguagaggag cugauacaag cguugggaua       60

guagguuugu uacuaacaac agcaauggca gcagaaguua caagaagagg gagugcauau      120

uacauguauc uagacaggaa cgaugcuggu gaagcaauuu cauucccaac aacauuagga      180

augaauaaau guuauauaca aauaauggac uuaggacaca caugcgaugc aacaauguca      240

uaugaguguc cgauguuaga ugagggagua gaaccagaug acguagauug uugguguaau      300

acaacaagua caugggucgu guauggaaca ugccaccaua agaaaggaga agcaagaaga      360

aguagacgag caguaacauu accaagucac aguacaagaa aacuacaaac uagaagucaa      420

acaugguuag aaucuagaga guauacaaaa cacuuaauca gaguagaaaa uuggauauuu      480

agaaauccag gauuugcauu agcagcugcg gcaauagcuu ggcuauuagg aagcucaaca      540

ucucaaaaag uaauauauuu aguaaugaua cuauuaauag caccagcuua uagcauaagg      600

uguauuggag uaucaaauag agauuucguu gaaggaaugu caggaggaac auggguagau      660

guuguucuag aacauggagg uugcguuaca gucauggcac aagauaaacc aacaguagau      720

auagaauuag ucacaacaac aguuaguaac auggcagaag uacgcucaua uuguuaugaa      780

gcaagcauaa gcgauauggc aucagauuca agauguccaa cacaaggaga agcauauuua      840

gauaaacaau cugacacaca auauguaugu aaacguacgu uaguagaccg uggauggggg      900

aacggaugug gauuauuugg uaaaggaucg uuaguaacau gcgcaaaauu cgcaugcagu      960

aagaaaauga ccggaaaaag cauacaaccc gagaacuuag aauauagaau aauguuaagc     1020

guacauggca gccaacauag uggaaugauc gucaacgaua caggacauga aacagaugaa     1080

aauagagcua aaguagaaau aaccccaaac agcccaagag cagaagcaac auuaggugga     1140

uuugguucac uaggauuaga uugcgaaccu agaacgggcc uggauuuuag ugaccuauac     1200

uauuuaacaa ugaauaauaa acacugguua guucauaaag aaugguuuca ugauauuccu     1260

cuaccauggc augcaggcgc agauacaggu acaccacacu ggaacaauaa agaagcauua     1320

guagaguuca aagaugcaca cgcaaagaga caaacugucg ugguacuagg uagucaagaa     1380

ggugcaguuc auacagcacu cgcaggagcg uuagaagcag aaauggaugg agcgaaagga     1440

agauugaguu caggucaucu aaaaugccga cuaaaaaugg auaaauuaag guuaaaaggu     1500

guaucauauu cauuaugcac agccgcuuuc acauuuacaa aaaucccagc agaaacuuua     1560

cauggaacag uaacuguaga agugcaauau gccggaaccg auggaccaug caaagucccu     1620

gcucaaaugg cuguagacau gcaaacauua acgccaguug gaagacuaau aacagcaaac     1680

cccguaauaa cggaaagcac cgaaaacagc aaaaugaugc uagaauuaga uccaccuuuu     1740

ggagacaguu auaucguuau aggcguaggc gaaaagaaaa uuacccauca uuggcauaga     1800

aguggaagca caauuggcaa agcauucgag gcaacaguaa gaggcgccaa aagaauggcu     1860

gucuuaggag acacagcaug ggauuuugga agugucggag gagcauuaaa uagccuugga     1920

aaaggaauac aucaaauauu uggggcagca uucaaaucac uauuuggagg aaugagcugg     1980

uuuucacaaa uuuuaauagg uacccuauua augugguuag gacuaaaugc uaaaaaugga     2040

aguauaaguu uaaugugucu ugcauuagga ggaguauuaa uauuucuauc aacggcugua     2100

ucagcugaug uaggauguag uguagacuuu ucaaaa                               2136


<210>  2570
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long CDS opt

<400>  2570
agaauaauca acgcucgaaa agaacgaaaa agaagaggag cagauacuag uauuggcaua       60

auaggucuau uacuuacuac cgcuauggca gcugaaauaa caagaagagg aagcgcauac      120

uacauguauu uagauagaag ugaugcagga aaagcaauuu cuuuugcaac gacauuggga      180

guaaauaaau gccacguuca aauaauggau cuaggacaua ugugugaugc uacuauguca      240

uacgaauguc cgauguuaga ugaaggagua gaaccagacg acgucgauug uugguguaau      300

acaaccagca cauggguagu guacggaaca ugccaccaua aaaaaggaga ggcaagacga      360

agcaggcgag caguaacauu gccgagucac ucaacaagaa aacuacaaac uaggucacaa      420

acaugguuag aaaguagaga auauacaaaa cauuuaauaa aaguggaaaa uuggauauuu      480

agaaauccag gauucgcucu aguugcugug gcaauagcuu gguuauuagg uaguaguacu      540

agucaaaaag ucauauaucu aguuaugauu cuacugauag caccagccua uucuauaaga      600

uguauaggag uaucuaacag agacuuugua gaagguaugu cgggaggaac uuggguagau      660

guaguacuag aacauggagg uuguguaacg gucauggcac aagauaaacc uacuguagac      720

auagaauuag uaacaaccac cguaucgaac auggcagaag uaagaucuua uugcuaugaa      780

gcaaguauua gcgauauggc aagcgacuca agaugcccua cucaaggaga agcauacuua      840

gacaaacagu ccgauacuca auaugugugu aaaagaacuu uaguagauag aggaugggga      900

aacggcugug gacuauuugg caaaggauca uuagugacau gugcaaaauu uacauguagc      960

aaaaagauga cagguaaaag uauacaacca gaaaauuuag aauacagaau aauguuaagu     1020

guacauggcu cucaacauag cggaaugaua ggauaugaaa cagaugagga uagagcaaaa     1080

guagaaguaa caccuaauag uccucgugca gaagcaacuc uggguggauu cggaagccua     1140

ggauuggauu gugaaccacg aacaggauua gauuuuagug aucuauauua cuuaacaaug     1200

aauaauaaac auuggcuagu ccauaaagaa ugguuucaug acauuccauu accauggcac     1260

gcaggugcag auaccggaac accacacugg aauaauaaag aagcuuuagu agaauuuaaa     1320

gacgcgcaug caaagaggca aacgguggua guauugggau cacaagaggg agcaguacau     1380

acagcucuag caggagcacu ugaagcagag auggacggug caaaaggaag auuauuuuca     1440

ggacacuuaa aauguagacu caaaauggau aaauuaaggu uaaaaggagu cagcuauagc     1500

uuaugcacag cagcauuuac auucacaaaa guaccagcag agacauuaca ugggacaguu     1560

acaguugaag uacaauaugc gggaacagau ggaccaugua aaauacccgu gcaaauggca     1620

guugacaugc aaacauuaac accuguagga agauuaauua cagcgaaccc aguaauaaca     1680

gaaucuacag aaaauaguaa aaugauguua gaacuagacc caccauuugg agauaguuau     1740

auagugauag gaguggguga uaaaaaaauc acacaucacu ggcauaggag uggaucuacu     1800

auuggaaaag cuuuugaagc aacaguaaga ggagcaaaaa gaauggcagu uuuaggagau     1860

acagcauggg auuucggaag cguaggaggg guauuuaaua gccuagggaa agguauucau     1920

caaauauuug gagccgcauu uaaaagucua uucgguggaa ugaguugguu cucacagaua     1980

uuaauaggaa cauuauuagu cugguuagga uuaaauacaa aaaauggguc aauaucucug     2040

acaugucuag cacuaggugg aguaaugaua uuucuaagua cagcaguaag cgcagauguc     2100

ggauguagcg uagacuuuuc gaaa                                            2124


<210>  2571
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long CDS opt

<400>  2571
agaauaauaa augcgagaaa ggaaaaaaaa cgacgaggag cagauacauc aguuggaaua       60

guaggcuuau uauuaacaac ggcaauggcc gcagaaguga caagaagagg aagugcauau      120

uacauguauu uagauagaaa cgaugcugga gaagcuauau cuuuuccaac cacauuagga      180

augaauaaau guuacauaca aauaauggau uuaggucaua ugugugaugc uacaauguca      240

uaugaguguc caauguuaga ugaaggugua gaaccugaug acguagauug uuggugcaac      300

acaacaagua cauggguagu auauggaaca ugucaucaua aaaaaggaga agccagaaga      360

agcagacgug cuguaacauu acccucacau agcacaagaa aacuacaaac ccgaagucaa      420

acgugguuag aaucuagaga auacacaaaa caucuuauaa gaguagagaa uuggauauuu      480

agaaauccug guuucgcauu ggcagcagca gcaauugcgu ggcuacuagg aucgucuaca      540

agucaaaaag uaauauacuu aguaaugaua uuacuuauag cgccugcaua uaguauaaga      600

uguauaggag uaagcaauag agauuuugua gaagguaugu caggggguac guggguagau      660

guaguauuag aacauggugg augcgucaca guaauggcac aagacaaacc uacaguagau      720

auugaacuag uaacaacaac aguaucaaau auggccgaag uaagaucaua uuguuaugaa      780

gcaucuauaa gugauauggc uagugacagu agauguccaa cacaagguga agcauaucua      840

gauaaacaaa gcgauacaca auaugugugu aaaagaacau uaguagauag aggauggggu      900

aacggaugug guuuauuugg aaaaggaucc uuaguaacgu gugcaaaauu ugcuuguagc      960

aaaaaaauga caggaaaauc aauccaacca gaaaauuuag aauauagaau cauguugucc     1020

gugcauggua gucaacacuc uggaaugauu guaaaugaua caggacauga aaccgaugaa     1080

aauagagcaa aaguugaaau aacaccgaau agcccaaggg cagaagccac auuaggagga     1140

uuuggcucgu uaggucuaga uugugaacca agaaccggau uagauuuuag ugaccuauau     1200

uaucuaacua ugaacaacaa acacugguua guucacaaag aaugguuuca cgauauaccc     1260

uuacccuggc augcuggggc agacacaggu acaccacauu ggaauaauaa agaagcacuu     1320

guagaauuca aagacgcuca ugccaagaga caaacuguag uugugcucgg uagucaggaa     1380

ggugcuguac acacagcacu ugcaggagca cuugaagcag aaauggaugg agcaaagggu     1440

agauuaucua guggucauuu aaaauguaga uuaaaaaugg acaaauuaag auuaaaaggc     1500

guaaguuauu cacuauguac ugcagcauuu acauuuacca aaauaccagc agaaacacua     1560

cauggaacug ucacaguaga aguacaguau gccggaacag augguccaug uaaagugccg     1620

gcucaaaugg caguugauau gcaaacauua acaccaguag gaagauuaau aacagcaaau     1680

ccgguuauua cagagucuac ggaaaauucg aaaaugaugc uugaacuaga cccaccauuc     1740

ggugacaguu acauugucau ugguguaggu gaaaaaaaaa uaacacacca uuggcauaga     1800

ucaggaagua caauaggaaa agcauuugaa gcuacaguga gaggagcgaa acgaauggca     1860

guacucggug auacagcaug ggauuuugga ucuguaggug gagcauuaaa uaguuuagga     1920

aaagguauac aucaaauauu uggagcagcu uuuaaaucac uauuuggagg aaugucaugg     1980

uuuucucaaa uucucauagg uacacuauua auguggcuag gccuaaauac aaaaaacgga     2040

agcauaaguu uaauguguuu agcuuuagga gguguuuuaa uuuuuuuaag uacagcggua     2100

ucagcagaug uaggauguuc aguugauuuu ucaaaa                               2136


<210>  2572
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long CDS opt

<400>  2572
agaauaauaa augcaagaaa agagcgaaaa cguagaggag cagauacaag cauuggaauc       60

auagguuuac uauugacaac agcaauggcu gcugaaauaa cuagaagagg cuccgccuau      120

uauauguacc uagacagguc agacgcaggu aaagccauaa gcuuugcaac aacauuaggc      180

guaaauaaau gucauguaca aauaauggau uuaggacaua ugugugacgc uacaaugagu      240

uaugaauguc caauguuaga ugaaggagug gaaccagacg auguugauug uugguguaau      300

accacuagua cauggguagu auaugguacg ugucaccaca aaaaaggcga agcaagaaga      360

uccagaagag cggucacauu accaucacau ucaacuagaa aacuacaaac aagaucccaa      420

acauggcuug aaagcagaga guacacuaaa caccuaauaa aaguagaaaa uuggauauuc      480

agaaacccug gauuugcauu gguagcagua gcaauagcau gguuauuagg aaguagcacc      540

ucacaaaaag uaauauaucu aguaaugauu cuacuaauug caccagcaua uucaauaaga      600

ugcauaggug uuuccaauag agauuuugua gaaggaaugu caggaggaac auggguagac      660

gucguccuag aacacggugg gugcguaacu guaauggcac aggauaaacc aacuguagau      720

auagaauuag uaacaacaac uguaucuaau auggcagaag uaagaucaua uuguuacgaa      780

gcaucaauau cugauauggc aagugauucc agaugcccaa cacaaggaga agcauauuua      840

gauaaacaau cagauacaca auauguuugu aaaagaaccc uggucgaucg agguuggggu      900

aauggaugug gucuuuuugg uaaaggaagc cuaguaacau gugcaaaauu uacauguagc      960

aaaaaaauga caggaaaauc uauacaacca gaaaaucuug aauauagaau aauguuaagu     1020

guucauggaa gccaacauuc aggcaugaua gugaaugaua cagguuauga aacagaugaa     1080

aacagggcga aaguagaagu aacaccaaac ucaccacgag cagaagccac uuuaggagga     1140

uucggaucau uaggacuaga cugugagcca agaacaggau uggauuuuag ugaucuauac     1200

uauuuaacaa ugaauaauaa acacugguua guacacaaag aaugguucca ugacauacca     1260

uuaccuuggc acgccggagc agacacagga acaccccacu ggaauaauaa agaagcgcua     1320

guagaauuua aagaugcaca cgccaaaaga caaacugucg uaguauuagg aucacaagaa     1380

ggugcaguac auacagcucu cgcaggagca uuagaagcag aaauggacgg agccaaaggu     1440

aaacuuuuuu caggacauuu aaaauguaga uuaaaaaugg auaaauugcg acuaaaaggu     1500

guaagcuauu cacuauguac agcagcauuu acauucacaa aaguacccgc agaaacacua     1560

cauggaaccg ugacaguaga aguucaauau gcaggaacag auggcccaug uaaaauaccu     1620

guacaaaugg caguagauau gcaaacacua acucccgucg gcagauuaau uacagcaaau     1680

ccaguaauaa cugaaaguac agaaaauuca aaaaugaugc uagaauuaga uccgccauuc     1740

ggagauucau acauaguuau uggcguggga gacaaaaaaa uuacacauca cuggcauaga     1800

agcggaagua ccauaggaaa agcuuuugaa gcaacaguuc gaggagcaaa aagaauggca     1860

guauuaggug acacugccug ggauuucggu uccguaggag gaguuuucaa uucauuaggc     1920

aaagguauuc accaaauauu uggugcggca uucaaaucau uauuuggagg uaugucaugg     1980

uuuagucaaa uacuaauagg aacauuacua guaugguuag gauuaaauac aaaaaacggg     2040

uccauaucuu uaacauguuu agcucuaggu ggcguuauga uauuuuuaag uacugcagua     2100

agugcagaug uuggcugcuc agucgauuuu ucuaag                               2136


<210>  2573
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long CDS opt

<400>  2573
agaauaauaa augcaagaaa agaaagaaaa agaagagggg cugauacaag uauagguaua       60

guaggacugc uauuaacaac ggcaauggcu gcagaaauaa caaggagggg aucagcauau      120

uacauguauc uagauagauc ugaugcggga aaagcaauau cuuuugcgac aacuuuagga      180

guaaauaaau gccauguaca gauaauggau uuaggacaua ugugcgaugc gacuaugagu      240

uaugaaugcc caauguuaga cgaagggguu gaaccagacg acgucgauug uugguguaau      300

acaacgagua cuuggguagu auauggaaca ugccaucaca agaaaggcga agcaagaaga      360

agcagacgag cgguaacauu accaucacau ucaacaagaa aacuacaaac aaggucacaa      420

acauggcuag aaagcagaga auauacaaaa cauuuaauca aaguagaaaa uuggaucuuc      480

agaaauccag guuuuacucu aguagccguc gcaauugcau gguuauuagg aaguaguaca      540

ucacaaaaag uaauauauuu aguaaugaua cuauuaauag cgccagcaua uaguaucaga      600

uguauaggag uaagcaauag agauuuugua gaaggaaugu caggagguac auggguagac      660

gugguacuug aacauggagg uuguguuacu guaauggcac aagauaagcc gacuguagau      720

auagaauuag uuacaacaac uguaaguaau auggcagaag uucgaaguua uuguuacgaa      780

gcaucgauaa gugauauggc aucagacuca agauguccua cacaagguga agcauaucua      840

gacaaacaau cagauacaca auacguaugu aaaagaacau uaguagaucg ugggugggga      900

aauggaugcg gauuguuugg aaaaggaagc cuaguaacau gcgcuaaauu uaccuguagu      960

aaaaagauga cuggaaaauc aauucagccg gaaaaccuag aauacagaau aaugcuauca     1020

guacacggaa gucagcauuc agguaugauc guaaaugacg aaaauagagc uaaaguagaa     1080

gugacgccua acaguccaag agcagaagcu acgcuaggag guuuugggag ucucggauua     1140

gauugugaac cuagaacagg auuggauuuu agcgauuuau acuacuuaac uaugaauaau     1200

aaacauuggc uuguacauaa ggaaugguuu caugacauac cauuaccaug gcaugcagga     1260

gcagauacag guacacccca uuggaauaau aaagaagcuc uaguugaguu caaggaugca     1320

caugcuaaaa gacaaacagu cguaguauug gggucacaag aaggugcagu acauacagcu     1380

cuugcuggag cacuagaagc ugaaauggau ggagcuaaag gaagacuguu uaguggucau     1440

uuaaaauguc gauuaaaaau ggacaaauua agauugaaag ggguaagcua cucuuuaugu     1500

acugcagcau ucacguucac uaaaguaccg gcugaaacau uacauggaac aguaacagua     1560

gaaguacaau augcggguac agauggacca uguaaaguac cagcucaaau ggcgguagau     1620

augcaaacac uaacaccagu ugggagauug auaacagcga acccaguaau aacggaaagu     1680

acagaaaaua guaaaaugau guuggaacuu gacccaccau ucggagauag uuauauaguu     1740

aucggaguag gagauaagaa aauuacacau cauuggcaua gaaguggaag uacaauuggg     1800

aaagcauuug aagccacugu uagaggggca aaaaggaugg caguuuuagg agauacagca     1860

ugggacuuug gaagcguagg cgguguuuuu aauagucuag guaaagggau acaucaaaua     1920

uuuggugcag cauucaaaag uuuauuugga gggaugucau gguucaguca aauucuaauu     1980

gguacacuau uaguaugguu aggucuaaau acgaaaaaug gaaguauuuc auugacaugu     2040

cuugcauuag guggaguaau gauuuuccua aguacagcug uuagugcaga ugugggaugu     2100

aguguagauu uuucaaaa                                                   2118


<210>  2574
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long CDS opt

<400>  2574
agaauaauaa acgcuagaaa agaaagaaaa agaagaggag cagauacauc cauaggaaua       60

auaggauuac uucucacaac agcgauggca gcagaaauca caagacgcgg aagugcauac      120

uauauguauc uagauagauc cgaugcagga aaggcgauau cauuugcaac gacauuaggc      180

guuaauaaau gucauguaca aauuauggau uugggccaca ugugugaugc uaccaugagu      240

uacgaauguc cuaugcuaga ugaaggagua gaaccugaug auguggauug uuggugcaac      300

acaacaucaa cauggguggu uuaugguacu ugccacagaa agaaagguga agcaagaaga      360

agcagacgag caguaacacu uccaucccau ucaacacgga aacuacaaac aagaucacaa      420

acaugguuag aaucuagaga auacacaaaa caucuuauaa aaguagaaaa uuggauuuuu      480

agaaacccag gauuugcauu aguagcuguu gcuauagcau ggcuucuagg uucaucaaca      540

ucacaaaaag uaauuuauuu aguuaugaua uuauuaauag cuccagcaua uaguauuaga      600

uguauuggag uaucgaauag agauuuuguu gaagguauga guggaggcac auggguggac      660

gucguauuag aacacggggg auguguaaca guaauggcac aagacaaacc aacaguugac      720

auagagcuag uaacuacaac aguaaguaac auggcagaag uaagaagcua uuguuaugaa      780

gccaguauca gugacauggc aagugacagu agauguccua cacaaggaga ggcuuauuua      840

gauaaacaaa gugauacaca guauguaugu aaaagaacau uaguagauag agguugggga      900

aauggcugug gacucuuugg aaaaggaucg cuaguaacau gugcaaaauu uacauguuca      960

aagaaaauga cuggaaaauc aauucaacca gaaaacuugg aauaccgaau aaugcuaagc     1020

gugcauggau cccaacauag cgggaugaua guaaacgaua caggauauga aacugacgaa     1080

aacagagcaa aaguagaagu aacuccaaau aguccaagag cagaagcaac cuuaggagga     1140

uuugguucuu uaggacuaga uugcgaaccc agaacaggau uagacuuuag ugaccuuuau     1200

uauuuaacca ugaauaacaa acauuggcua guucauaaag aaugguuuca ugauauacca     1260

uuaccauggc augcaggcgc agauacuggc acuccacauu ggaacaauaa agaagcgcua     1320

guagaauuua aagacgcuca ugccaagaga caaacagugg ucguacuugg aagccaagaa     1380

ggcgcagugc auacagcucu agcaggagca cuagaagcag aaauggaugg ugcaaaagga     1440

aaguuauuua guggacauuu aaaauguaga cuaaaaaugg auaaauuaag auuaaaagga     1500

gucagcuacu cgcucugcac cgcagcauuu acauuuacaa aaguaccagc agaaacauua     1560

cauggaacag uuacaguuga aguacaauau gcagguacag auggaccaug uaaaauacca     1620

guucaaaugg cuguagacau gcaaacauua acgccuguag ggagacuaau aacagcaaau     1680

ccagucauaa cggaaucaac agaaaacagu aaaaugaugc uagaacucga cccuccauuc     1740

ggagacucau acauaguaau uggaguugga gacaagaaaa uuacucacca uuggcacaga     1800

ucagggucaa caauaggaaa agccuucgaa gcaacuguaa gaggagcuaa aagaauggcu     1860

guauuaggcg acacagcaug ggauuuugga uccgucggag gaguauuuaa uagcuuagga     1920

aaaggaauac accaaauauu uggagcggcu uuuaaaucac uuuuuggcgg aaugucaugg     1980

uuuucacaaa uacuuauagg aacucuauua guaugguuag gccuaaauac uaaaaaugga     2040

aguauaucau uaacaugucu agcauuagga ggagucauga uauucuuaag cacagcagua     2100

agugccgacg uaggaugcuc aguagauuuu aguaaa                               2136


<210>  2575
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long CDS opt

<400>  2575
agaauaauaa augcaagaaa agaaagaaaa aggagaggug ccgauacguc uauagguaua       60

auaggauuau uacuaacuac agcaauggcg gcagaaauaa cacgaagagg aagugcguac      120

uacauguauc uagacagaag ugacgcagga aaagcaauua gcuucgcaac aacacuuggu      180

guaaauaaau gucaugucca aauaauggau uuaggacaua ugugugaugc cacaauguca      240

uaugaauguc caaugcuaga ugaaggagug gagcccgaug acguugacug uugguguaau      300

acaacaagca cuuggguagu auaugguacu ugccaucaua agaaagguga agccagaaga      360

agcagacgag caguuacauu accaucacac agcacuagaa aauuacaaac aaggagucag      420

acuugguuag aaagucgaga auacacaaaa caccuaauua aaguagaaaa uuggauauuu      480

agaaacccug gcuucgcauu aguagcaguu gcaauagcau ggcuacuagg uaguaguaca      540

agucagaaag uaauuuauuu agucaugauc cuauugauag caccagcaua uucaauaaga      600

uguauugggg uuucaaauag agauuucgua gaggggauga guggcggaac auggguagac      660

guaguauuag aacauggagg cuguguuacg guaauggcgc aagauaaacc aacugucgau      720

auagagcuag uaacaacaac aguaucaaau auggcagagg uaagaucuua cuguuaugag      780

gcaaguauaa gugauauggc aucagacagu agauguccua cacaaggaga agcauauuua      840

gauaaacaau cggauacaca auauguaugu aaaaggacgc uaguagaucg aggaugggga      900

aacggaugug gauuauucgg aaaaggaagu uuaguuacau gcgcaaaauu cacauguuca      960

aagaagauga caggaaaauc aauacaacca gaaaaccuag aauacagaau aauguuaucu     1020

guacacggaa gucagcauuc aggaaugaca guaaaugaca uaggauacga gacugaugaa     1080

aauagagcua aaguagaagu aacuccaaau ucaccgagag cagaagcuac auuaggagga     1140

uuuggaaguc uaggacuaga cugugaaccu cgaacagguu uagauuuuag ugauuuauau     1200

uacuuaacaa ugaacaacaa acacuggcua guucauaaag aaugguuuca ugacauacca     1260

uuacccuggc acgcuggugc cgauacagga accccacauu ggaacaauaa agaagcauua     1320

guagaauuua aagaugcaca ugcaaaaaga caaacaguag uagucuuggg uucacaagaa     1380

ggcgccguac auacggcauu agcaggggcu uuagaagcag agauggaugg cgcaaaaggu     1440

aaauuauuuu cgggacaccu aaaaugcaga uuaaaaaugg auaaauuacg auuaaaagga     1500

guaucauauu cacuauguac agcagcauuu acauuuacaa aaguaccagc ugaaacucug     1560

cacggaacag ugacgguaga aguucaauac gccgguacag auggaccaug caaaauaccu     1620

guacaaaugg cuguagauau gcaaaccuua acaccaguag gaagauuaau uacugcaaau     1680

ccaguaauua cagagagcac cgagaauagc aaaaugaugc uagaauuaga uccaccuuuu     1740

ggugauucau auauaguaau cgguguugga gauaagaaaa uaacacacca cuggcauaga     1800

ucaggaucaa caauagguaa agcauucgaa gcaacaguaa gaggcgcaaa acgaauggcu     1860

guacuaggug auacagcuug ggauuuugga ucaguaggag gaguauuuaa cagucuagga     1920

aaaggaauac aucagauuuu uggggcagca uucaaaaguu uauuuggagg caugagcugg     1980

uucagucaaa uauuaauugg aacacuuuua guauggcuag gauuaaauac gaaaaauggu     2040

aguauaaguc ugacaugucu agcauuaggu gguguaauga uauuucuuag cacagcgguc     2100

agcgcagaug uaggauguuc uguggacuuu aguaaa                               2136


<210>  2576
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long CDS opt

<400>  2576
cgcauaauaa augccagaaa agaaagaaaa aggagaggug cagauacauc cauaggaaua       60

auaggacugu ugcuaacgac cgcaauggca gcagaaauua caaggagggg cucagcauau      120

uauauguauc uagauagguc ugaugcagga aaagcaauaa guuuugcaac aacacuaggg      180

guaaauaaau gccauguuca aauaauggau uuaggacaua ugugugaugc aacaaugagu      240

uacgaaugcc cuaugcuaga cgaaggagug gaaccggacg acgucgauug cugguguaau      300

acaacuucaa cauggguugu auaugguacu ugccaucaua aaaaaggaga agcaagaaga      360

agccgaagag caguaacacu accaagccau agcacucgaa aauugcaaac aagaucucag      420

acauggcuag aaucaagaga auauacaaaa cauuuaauca aaguagaaaa uuggauauuu      480

agaaauccag gauucgcccu aguagcagug gcaauagcau gguuauuagg aucuucaaca      540

agucaaaaag ucauauauuu agugaugauu uugcuaauag cuccagcaua uucuauaaga      600

uguauaggag uaucaaacag agacuuuguu gaaggaauga guggcggaac auggguagau      660

guaguauuag aacauggagg augugugaca guaauggcuc aagauaaacc aacuguugau      720

auugaacuug uaacaacaac aguaucuaac auggcugaag uaagaaguua uuguuaugag      780

gcuucuauau cagauauggc cucagauagu aggugcccua cacaaggaga agcauacuua      840

gauaaacaaa gugacacaca auacguaugu aaacgaacuu uaguagauag agguugggga      900

aauggcugug gucuauuugg aaaaggaagu cuaguaacau gugcaaaauu uacauguuca      960

aaaaaaauga cuggaaaauc aauucaacca gaaaauuuag aguauagaau aaugcuauca     1020

guacauggaa gucaacacag ugggaugaua ggauacgaaa cagacgaaaa uagggccaaa     1080

guagaaguaa caccgaacuc uccaagagcg gaagcgacau uagggggauu cggaucauua     1140

ggauuagauu gugaaccaag aacaggauua gauuuuagcg aucuauauua cuuaacaaug     1200

aauaacaaac auuggcuagu acauaaagaa ugguuucaug auauaccucu accauggcau     1260

gcaggagcag auacugguac gccacauugg aacaauaaag aagcauuggu agaauuuaaa     1320

gaugcacaug cuaagaggca aacgguugua guacucggau cacaagaagg agcaguacau     1380

acugccuuag cuggugcacu agaagcagaa auggauggug caaaaggaaa acuauuuagu     1440

ggacauuuaa aaugucgacu aaaaauggac aaacuaagac uaaaaggagu uucauauucu     1500

uuaugcacag ccgcauuuac auucacaaaa gucccagcag aaacacuuca cggaacagug     1560

acaguugaag uacaauaugc aggaacggau ggaccgugua aaauaccugu acaaauggca     1620

guagauaugc aaacacuaac accaguagga agauugauaa cagcaaaccc aguaauaaca     1680

gaaucaacag aaaacucaaa aaugauguua gaguuggauc cgccauucgg agauaguuau     1740

auuguuauag gaguaggcga caagaaaaua acucaucauu ggcauagauc aggaagcacu     1800

auagguaaag cguucgaggc aacggugaga ggggcuaaaa gaauggcagu auuaggagau     1860

acagcauggg auuuuggaag uguaggagga guauucaacu cacuaggaaa aggaauacau     1920

caaauauuug gagcagcuuu uaaaucacua uucgguggca ugagcugguu uucacaaaua     1980

uuaauaggca cacuauuagu augguuagga cuaaacacaa aaaauggaag uauaagucua     2040

acauguuuag cacuaggagg aguaaugaua uuucuaucaa cagcagugag ugcugaugua     2100

gguugcagug uugauuucuc gaaa                                            2124


<210>  2577
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long CDS opt

<400>  2577
agaauaauaa augcaagaaa agaaaagaaa agacguggag cugauacauc aguaggaaua       60

guaggauuac uauuaacaac ugcgauggcc gcagagguaa cacgaagagg aucagccuac      120

uauauguauc uggacagaaa ugaugcagga gaagcaauaa guuuuccaac uacauuaggu      180

augaauaaau guuauauaca aauuauggac uuaggacaua ugugcgaugc uacaauguca      240

uacgaaugcc caauguuaga ugaaggugua gaacccgacg auguugauug uugguguaac      300

acaacaagua cauggguagu uuacgggacc ugucaccaua agaaaggaga agcgagaaga      360

ucaaggagag cagucacucu gccaagccac ucuacaagaa aauugcaaac aagaagucaa      420

acauggcuag aaaguaggga auacacaaaa cauuuaauca gaguagaaaa cuggauauuu      480

aggaauccag gcuuugcacu agcagcagca gcaaucgcuu gguugcuagg uagcaguaca      540

agucagaaag uaauauauuu aguaaugauu uuauuaauag caccagccua uucaauucga      600

uguauaggug ucagcaauag agauuuugua gaaggaaugu cuggcggaac auggguugau      660

guaguacuug aacauggagg augugugaca guaauggcuc aagacaaacc aacuguagac      720

auagaauuag uaaccacuac uguaaguaau auggcagaag uaagaaguua uuguuaugag      780

gcuucaauau cagauauggc aucagacuca agauguccaa cacaaggaga agcauaucuu      840

gacaaacaau cagauacgca auauguaugu aaaagaacuc uagucgauag aggauggggu      900

aacggaugug gauuauucgg aaaagguagc uugguaacuu gugcaaaauu ugcauguagu      960

aagaaaauga cagggaaaag uauacaaccc gaaaaucuag aauaucgaau aauguuaagu     1020

gugcauggau cgcaacauag uggaaugaua guaaaugaua caggacauga aacagaugaa     1080

aauagagcua aaguagaaau aacaccaaau ucaccgagag cagaagcaac cuuaggaggg     1140

uuuggaaguu ugggauuaga uugugaaccc agaacaggcu uagacuuuuc agaucuauau     1200

uaccuaacua ugaacaauaa acauugguua guacauaaag aaugguuuca ugauauaccu     1260

uuaccauggc augcaggagc agauacuggu acaccgcauu ggaauaauaa agaagcauua     1320

gucgaauuua aggaugcaca cgcaaaaaga caaacaguag uaguacuagg aucucaggaa     1380

ggcgcagugc auacagcacu cgcuggugca uuagaagcag aaauggaugg agcaaaagga     1440

agauuaagcu cgggacaucu aaaauguaga uuaaaaaugg acaaauugag auuaaaaggu     1500

guaagcuaca guuuaugcac agccgccuuc acauucacaa aaauaccugc agaaacauua     1560

cauggaaccg uuacgguaga aguacaauac gcaggaacag auggaccaug uaaaguccca     1620

gcacaaaugg caguagauau gcaaacauua acaccaguag guagauugau aacugcaaau     1680

ccaguaauua cggaaucgac agaaaauagu aaaaugaugc uagaauuaga uccaccauuc     1740

ggcgauaguu auauaguaau aggaguugga gagaaaaaga uaacacauca cuggcaucga     1800

agugguucaa caauaggcaa agcauucgaa gcaacaguca gaggagcaaa aagaauggcu     1860

guauuaggcg acacagcgug ggacuucgga aguguaggug gagcacuaaa uucauuagga     1920

agaggaauac aucaaauauu uggagcagca uuuaaaucac uauuuggagg uaugagcugg     1980

uuuagucaaa uauuaauugg aacauuauua augugguuag gacuaaauac uaaaaauggu     2040

ucuauaaguu uaauguguuu agcacuagga ggaguacuaa uuuuucucag uacagcugua     2100

ucagcagaug uugguuguuc aguugauuuc agcaaa                               2136


<210>  2578
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long CDS opt

<400>  2578
agaauaauaa acgcacgaaa agagaaaaag agaagaggcg cagaugccag cguaggaaua       60

guaggauuac uacuaacaac ugcaauggca gccgaaguca cucgaagagg aagugcauau      120

uauauguauu uagauaggaa ugaugcaggg gaagcaauau cauuccccac aacauuagga      180

augaauaaau gcuacauaca aauaauggau uuaggacaua ugugugacgc aacgauguca      240

uacgaauguc cuauguuaga cgagggaguu gaaccagaug acguagauug cuggugcaau      300

acaacaucaa cauggguugu auauggcacu ugucaccaua agaaaggaga agcgagaaga      360

ucuaggagag caguaacacu accaucacau aguacgagaa aacuacaaac aagaucucaa      420

acaugguuag aaucaagaga auauacaaaa cacuuaauaa gaguagaaaa cuggauauuc      480

agaaacccag gauucgcauu agcagcagca gcaauugcgu gguuacuagg aaguaguaca      540

agccaaaaag uuauauaucu ugucaugauu cuauuaauag caccagcaua uaguauuagg      600

ugcauaggcg uuagcaacag agauuucgua gaagggaugu caggaggaac auggguagau      660

guuguauuag agcauggggg augugucacc guaauggcac aggauaaacc uacaguagac      720

aucgaauuag uuacaacaac aguaucaaau auggcagaag uaagaucuua uugcuaugaa      780

gcaucaauau cagacauggc uagugauagc cgaugcccua cacaaggaga agcauacuua      840

gauaaacaau cagauacaca auauguaugu aaaagaaccc uaguagauag aggaugggga      900

aauggaugug gauuauucgg aaaaggauca uugguaacuu gugcaaaauu ugcauguuca      960

aagaaaauga cuggaaaaag cauacagccu gaaaaucuug aauacagaau aaugcuuagu     1020

guacacggau cucaacauuc agggaugaua guaaacgaua cagggcauga aacagaugaa     1080

aauagagcga aaguagaaau uacaccuaau agucccagag cugaagcaac acuaggagga     1140

uucggaagcc uaggacuuga uugugaaccc cguacaggau uagauuuuuc agacuuauau     1200

uauuugacaa ugaacaauaa acauuggcua guccacaaag aaugguucca cgacauaccg     1260

cuacccuggc acgcaggagc ggacacuggu accccacauu ggaauaauaa agaagcauua     1320

guagaguuca aagaugcaca ugcuaaacga caaacaguag uaguacuugg aagucaagaa     1380

ggagcaguac auacagcauu agcgggugca cuagaagcag aaauggaugg agcaaaagga     1440

agauuaagua gugggcaucu aaaaugcaga cucaaaaugg auaaacuaag guuaaaagga     1500

guaucauauu cauuauguac agcagcuuuu acauuuacua aaauaccugc agaaacguua     1560

cauggaacgg uaacugucga aguacaauau gcuggaaccg acggaccaug caaaguaccu     1620

gcacaaaugg caguagauau gcaaacauua acuccuguag gcagauugau aacugcaaac     1680

ccugucauaa cggaaucaac agaaaauagu aaaaugaugc uugaauuaga cccaccauuu     1740

ggagauaguu auauaguaau cggaguaggg gaaaagaaaa uaacacauca cuggcacagg     1800

ucggggucaa caauaggaaa agcguuugaa gcaacaguaa gaggagcaaa aaggauggca     1860

guauuaggug acacagcuug ggauuuugga agcguaggug gugcauuaaa uaguuuagga     1920

aaaggcauuc aucaaaucuu cggagcggca uuuaaaaguc uauucggagg aaugucuugg     1980

uuuucucaaa uacuuauugg aacuuuauua auguggcuag gauuaaauac uaaaaacggu     2040

aguauaaguu uaaugugccu cgcauuggga ggaguauuaa uauuucucag uacagcggua     2100

agcgcagacg uaggguguag cguagauuuc ucuaaa                               2136


<210>  2579
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long CDS opt

<400>  2579
agaaucauaa augcucggaa ggaaaaaaaa agaagaggag ccgauacaag cguaggaaua       60

guaggauuac uucuaacgac agcgauggcg gcagagguca caaggagagg auccgcauau      120

uacauguacu uggauagaaa ugaugcagga gaagcaauau cauuuccaac aacacuagga      180

augaacaaau guuauauaca aauaauggau cuaggacaua ugugcgaugc uacaauguca      240

uaugagugcc caauguugga ugaaggagua gagccagaug auguagacug uugguguaac      300

acaacaucaa cuuggguugu auacgggacg ugucaucaua aaaaagggga ggcgaggagg      360

ucgagaagag caguaacguu accaucacau agcacaagaa aauuacaaac acgaagucaa      420

accuggcuag aaaguagaga auauacaaaa cauuuaauua gaguagaaaa uuggauauuu      480

agaaacccag gauuagcguu agcugcagca gcaauagcau ggcuuuuagg uagcucgaca      540

agucaaaaag uuauauaucu cguuaugaua cucuuaauag caccugcaua uaguauaaga      600

uguauuggag uaaguaaucg agacuuugua gaagguaugu caggaggaac augggucgau      660

gugguacugg aacauggagg auguguaacu guuauggcac aagauaaacc aacaguagac      720

auagaguuag uaacaacaac cguaucaaac auggcagaag ugcguaguua uugcuaugaa      780

gcgucgauau cagacauggc aagugauagc cgauguccaa cucaaggaga ggcauauuua      840

gauaaacaaa gugauacuca auauguaugc aaaagaacac uagucgauag agguuggggu      900

aacggaugcg gauuauucgg gaaaggcuca uuaguaacuu gugcuaaauu ugcuuguuca      960

aaaaaaauga cuggaaaaag cauucaacca gagaauuugg aauaucguau aauguuaagu     1020

guucauggau cacaacacuc agguaugaua gucaaugaua caggacauga aacagacgaa     1080

aauagggcaa aaguagaaau aacaccaaau aguccuagag cagaagcaac auuaggcgga     1140

uuugggaguu uaggcuuaga cugugagcca agaacuggau uagacuuuag cgacuuauau     1200

uacuuaacaa ugaacaacaa acauugguua guacacaaag agugguuuca ugauauaccg     1260

uuaccauggc augcaggagc agacacugga acaccacauu ggaauaauaa agaggcauug     1320

guagaauuua aagaugccca ugcgaaacga caaacagugg uaguacuagg aucacaagaa     1380

ggcgcaguac auacagcacu ugcuggcgcc uuggaagcag aaauggacgg ggcuaaaggu     1440

agauuaaguu caggucauuu aaaauguaga uuaaaaaugg acaaauuaag auuaaaaggu     1500

gugucauauu cauugugcac agcagcauuc acauuuacua aaauaccagc ugaaacauua     1560

cacgguacag uaacaguaga aguucaauau gcagguacgg acggcccaug uaaaguccca     1620

gcucaaaugg cgguagauau gcagacauua acuccaguug guagacuaau aacagcaaau     1680

ccuguaauca ccgaaaguac ugaaaauuca aaaaugaugu uggaacuuga uccaccauuu     1740

ggagauucau auauuguaau agguguaggg gaaaaaaaaa uaacacauca uuggcacaga     1800

aguggaagua cgauuggaaa agccuuugaa gcaacgguaa ggggugcaaa acgaauggca     1860

guauuaggug auacagcaug ggauuuuggc aguguaggag gugcacuaaa uucauuagga     1920

aaaggaauac aucaaauauu cggagcagca uuuaaaagcu uguuuggugg uaugucaugg     1980

uuuucacaaa uacuaauagg aacacuauua augugguuag gauuaaauac aaaaaauggg     2040

aguauaaguc ucaugugucu ggcauuaggc ggaguacuca uauuuuuaag uacagcaguu     2100

agcgcagaug uaggauguag uguagauuuu aguaaa                               2136


<210>  2580
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long CDS opt

<400>  2580
cgaauaauaa augcacgaaa agaaaagaaa aggagaggag cugacacuuc cguaggcauu       60

guaggauuac ucuuaacaac ugcuauggca gcugaaguca ccagaagagg gucagcauau      120

uacauguauc uugauagaaa ugacgcaggg gaagcaauua gcuuuccaac aacacuugga      180

augaauaaau guuacauaca aauaauggac cucggacaua ugugcgacgc aacuaugagc      240

uaugaauguc caaugcuaga cgaaggagua gaaccagaug acguagacug uuggugcaau      300

acuacaagua cuuggguagu uuaugggaca ugccaucaua agaaagguga agcaagaaga      360

ucuaggagag caguuacauu accuagucau agcacgagaa aacuacaaac aagaucccaa      420

acaugguuag aaucaaggga auacacaaaa caucuuauaa gaguagaaaa uuggauauuc      480

agaaaucccg guuucgcucu agcagcagcu gcuauagcuu ggcuauuggg aaguagcacu      540

agccaaaaag uaauauacuu aguaaugaua cuauuaauag cuccugcaua uucuauaaga      600

uguauuggag uaaguaacag agacuucguu gaaggaauga guggaggcac auggguagau      660

gucguacuug aacacggagg auguguaaca guaauggcac aagauaaacc gacaguagau      720

auugaauugg uaacaacaac agucaguaau auggcagaag uuagaaguua uuguuaugaa      780

gcuaguauaa gugacauggc aucagauuca cgauguccua cacaaggaga ggcuuaucua      840

gacaaacagu cugauacaca auacgugugu aaaagaacuu uaguugaucg aggaugggga      900

aauggaugug gacuauuugg aaaaggauca uuaguaacau gugcaaaauu cgcauguuca      960

aagaaaauga caggaaaaag cauacaacca gaaaauuuag aauacagaau aauguuaagu     1020

gugcauggau cccaacacag cggcaugaua guaaaugaua caggacacga aacagaugaa     1080

aacagagcaa aaguugaaau uacaccaaau aguccacgag cggaagccac acuuggaggg     1140

uucggaucac uaggacuuga cugugaacca agaacuggau uagauuucuc agauuuauau     1200

uacuuaacaa ugaauaacaa acauugguug guacauaaag aaugguuuca cgauauuccg     1260

cuaccauggc augcuggagc agauacaggg acaccacauu ggaauaacaa agaagcucua     1320

guagaauuua aagaugcaca ugcaaagaga caaacaguag uaguacuagg aagucaggaa     1380

ggagcaguac auacagcacu agcaggagcu cuagaagcug aaauggaugg ugcaaaagga     1440

agacuaagua gcggacaucu aaaauguaga cuaaaaaugg acaaacuaag acuaaagggg     1500

guuucauauu cauuauguac agcagcuuuu acauucacaa aaauaccugc agaaacuuua     1560

caugguacag uaacaguuga aguacaauau gcuggaacag acggacccug uaaaguacca     1620

gcacaaaugg caguagauau gcaaacauua acaccaguug gacgauuaau aacagcaaau     1680

cccguuauaa ccgaaaguac agaaaauucu aaaaugaugu uagaauuaga ucccccauuc     1740

ggagacagcu auauagugau uggaguagga gaaaagaaaa uuacacauca cugguaccgc     1800

ucaggaucaa caauaggaaa agccuucgaa gcuacuguaa gaggugcaaa acgaauggca     1860

guacuagggg acacagcgug ggauuucggu ucaguaggag gagcacuuaa cuccuuaggu     1920

aaaggcauac aucaaauauu uggagcagcu uuuaaaucuc uauuuggggg caugucaugg     1980

uucucacaaa uuuuaaucgg cacacuacua augugguuag gacuaaauac uaaaaacgga     2040

ucaauaaguc ucaugugucu agcccuagga ggaguacuaa uauuucuaag uacugcugua     2100

ucagccgaug ugggaugcag uguagauuuc agcaaa                               2136


<210>  2581
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long CDS opt

<400>  2581
agaauaauua augcacgaaa agaaaaaaaa agaagaggag cugauacaag uguaggcaua       60

guagguuuac uguuaacuac cgcuauggca gcagaaguaa cgagaagagg aucagcauau      120

uauauguauu uagauagaaa ugaugcuggg gaagcaauau cauuuccuac aacauuagga      180

augaauaaau gcuauauuca aauaauggau cugggacaca ugugugaugc aacaauguca      240

uaugaaugcc ccauguuaga ugaaggugua gaaccagaug auguagacug uugguguaau      300

acaacaucaa cuuggguagu auauggaaca ugccaucaua aaaaaggaga agcgagaaga      360

agcagaagag cuguaacauu accaucacau aguacuagaa aacuacaaac aagaagccaa      420

acauggcuug aaaguaggga auauacuaaa caucuuauaa gaguagaaaa uuggauauuu      480

agaaauccag gauucgcauu agcagcugca gcuauagcau ggcuauuagg aucauccaca      540

agucaaaaag uaauauauuu gguaaugaua uuacuaauug caccagcaua cucaauaaga      600

uguauaggag ucaguaauag agauuuugug gaaggaauga gcggaggaac auggguagac      660

guaguuuuag aacacggugg augcguuaca guuauggcac aagauaaacc aacuguagac      720

auagaauuag uuacaaccac aguaaguaau auggcagaga uuagaucaua uuguuaugaa      780

gcauccauaa gcgauauggc aucggauagu agauguccaa cacagggcga agcauaccua      840

gauaaacaaa gcgauacgca auauguuugu aaaagaacgc uaguagauag aggauggggu      900

aaugguugug gacuuuucgg aaaaggaagc cugguaaccu gugcaaaauu cgcauguucu      960

aaaaaaauga caggaaaauc uauucaacca gaaaaucugg aauauagaau aauguuaagc     1020

guccauggaa gucaacauuc cggaaugauu guaaaugaua cgggacauga aacagacgaa     1080

aacagagcaa aaguugaaau aacaccaaau uccccaagag cagaagcaac ccuaggagga     1140

uuuggaucau uaggauuaga uugcgaaccu agaacaggac uagacuucuc agaccuguau     1200

uaccuaacaa ugaacaauaa acauugguua guacauaaag aaugguucca ugauauacca     1260

cuaccguggc augcaggagc ugacacugga acaccccauu ggaauaacaa agaagccuua     1320

guagaguuua aagaugcaca cgcaaaacga caaacaguag ucguccuagg uagucaagaa     1380

ggugcuguac auacagcauu agcuggugcu cuagaagcag aaauggaugg agcuaaagga     1440

agacuaucaa guggacaccu aaaaugccga uuaaaaaugg auaaauuaag acuaaaagga     1500

guaaguuaca guuuaugcac agcggcauuu acauuuacaa aaauacccgc agaaaccuua     1560

caugguacag uaaccguaga aguacaauau gcaggcacgg auggaccaug uaaaguacca     1620

gcacaaaugg cuguugauau gcaaacacua acaccgguag gccgauuaau aacugcuaac     1680

ccaguaauca cagaaaguac ugaaaauagu aagaugaugu uagaauugga cccaccauuu     1740

ggagacucau auauaguaau cggcguagga gaaaaaaaaa uuacacauca cuggcauaga     1800

ucaggaagua caauaggaaa agccuucgaa gcaacuguaa gaggagcaaa gagaauggca     1860

guacuaggag auacagcaug ggauuuugga ucgguuggag gagcucucaa uagcuuggga     1920

aaaggcauac aucaaauauu uggagcagcc uuuaaaaguu uguucggagg aaugaguugg     1980

uuuucucaaa uauuaaucgg gacacuacua auguggcugg gauuaaauac aaaaaauggc     2040

ucaauaaguc ucaugugcuu agcuuuaggg ggcguuuuaa uauuuuuaag uacagcagua     2100

ucagcagacg uugguugcag cguagauuuu agcaaa                               2136


<210>  2582
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long CDS opt

<400>  2582
agaauaauaa augcccgaaa agaaaaaaaa cgaagaggag ccgacacaag uguaggaauc       60

guaggguuau uacuuacgac ggcuauggcg gcagaaguaa cacgaagagg aagugccuac      120

uacauguacu uggacagaaa ugacgcagga gaagcaauau cauuuccaac aacacuaggu      180

augaacaaau guuauauuca aaucauggau cuaggucaua ugugugaugc cacuaugagc      240

uacgaaugcc caaugcuuga ugagggcgua gaaccugaug auguggacug uuggugcaac      300

acaacuagua cuugggucgu guacggcaca ugucaucaua aaaaaggaga agcaagaaga      360

agcaggagag caguaacguu accgagccau ucaacaagaa aacuacaaac aagaagccaa      420

acaugguuag agagucgaga auauacaaaa cacuugauaa gaguagaaaa cuggauauuu      480

agaaauccag guuucgcauu agcagcagca gcaauugcau ggcuauuggg uaguuccaca      540

agucaaaaag uaauuuauuu aguuaugaua uuauuaauag cucccgcaua cucaauaaga      600

ugcauaggag uaagcaauag agauuuugua gagggaaugu ccggaggaac guggguagau      660

guagucuuag aacauggugg auguguaacu guaauggcac aagauaaacc aacuguagau      720

aucgaacuug uaaccacaac cguaucaaau auggcagaag uuagaucgua cugcuaugag      780

gccucaauau cagauauggc aucagauuca agauguccaa cacaagguga agcauaucuu      840

gacaaacaau cugauacuca auauguaugu aaaaggacac uaguagauag agguuggggu      900

aauggcugug gacuauuugg uaaaggaucu cuaguaacau gugcaaaauu ugcauguuca      960

aaaaaaauga caggaaaaag uauacaacca gaaaaucuag aauacagaau aauguuaucc     1020

guacauggga gccaacauag cggaaugaua guaaaugaua cuggacacga aacagaugaa     1080

aaucgagcaa aaguagaaau aacucccaau ucaccuagag cugaagcaac ccuaggaggu     1140

uuuggaaguu uaggauuaga uugugaacca cgaacagguc uagauuuuuc agaucuauau     1200

uauuuaacaa ugaauaacaa acacugguua guacauaaag aaugguuuca ugauauacca     1260

uuaccauggc acgcaggggc agacacagga acuccccacu ggaacaacaa agaagcuuua     1320

guggaauuua aagaugcaca ugcaaaaaga caaacaguag uaguacuagg cucucaagaa     1380

ggagcugugc auacugcauu agcaggagcc uuagaagcag aaauggaugg agcaaaaggg     1440

agauugucau cagggcauuu aaaaugucga cuaaaaaugg auaaauuaag auuaaaaggg     1500

gucagcuauu cacuauguac ggcagcuuuu acauuuacaa aaauaccagc agaaacacuc     1560

caugguacag uaacaguaga aguucaauau gcagguacag auggaccaug uaagguucua     1620

gcacaaaugg caguagauau gcaaacguua acuccuguag gaagauuaau aacagccaau     1680

ccuguaauaa cagaaucaac cgaaaauuca aaaaugaugu uagaauuaga uccuccuuuu     1740

ggagauucuu auauaguaau agguguuggu gaaaaaaaaa uaacacauca uuggcauaga     1800

aguggaagca caauagguaa agcauuugaa gcaacaguaa gaggagcaaa acgaauggcu     1860

guacuaggag acacagcuug ggauuucgga aguguaggcg gagcauuaaa uucauuagga     1920

aaggguauac aucaaaucuu uggggcugca uucaaaaguu uauuuggugg aaugagcugg     1980

uucucacaaa uucuuaucgg aacacuauua auguggcuag gucuuaauac aaaaaauggg     2040

ucaauaucuu uaauguguuu agcauuaggg ggaguacuua uauuccucuc aacagccgua     2100

ucagcagacg uuggauguuc aguagauuuu ucaaaa                               2136


<210>  2583
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long CDS opt

<400>  2583
agaauaauaa augccagaaa agaaaaaaaa agacguggug cugacaccag uguaggaaua       60

guaggauuau uguuaacaac ugcaauggcg gcagaaguaa caagaagggg aagugcauau      120

uauauguauc uagauagaaa cgacgcaggc gaagcaauaa guuucccaac aacauuagga      180

augaacaaau guuauauaca aauaauggac cucgggcaca ugugcgaugc aacaaugagu      240

uaugaaugcc caacacuaga cgaaggaguu gaaccggaug acguagauug uuggugcaac      300

acaacaagua cguggguugu auaugguacg ugucaucaua aaaaagguga agccaggagg      360

ucaagaaggg caguaacauu gccaucccau aguacacgga aauugcaaac aagaucacag      420

acuuggcuug aaagcagaga auauacaaaa cauuuaauaa gaguagaaaa uuggauauuu      480

cggaauccag gauuugcauu agcagcugcu gcaauugcau ggcuucuagg uucgucaaca      540

ucacaaaaag uaauauauuu gguaaugaua uuguuaauug cuccagcaua uucaauucgu      600

uguauaggug uaagcaaucg ugauuucgua gaaggaauga gcggagggac guggguugau      660

gucguguuag aacauggagg guguguaaca guaauggcac aagauaaacc aacaguagau      720

auagaauuag uaacaacuac aguaagcaac auggcagaag uaagaucaua uuguuaugaa      780

gcaaguauau ccgacauggc cuccgauagu agauguccca cacaagguga agcguauuua      840

gauaagcaau cugauacaca auacguaugc aaaagaacac uaguagauag agguugggga      900

aauggcugcg gauuauuugg aaaaggcagu cuaguuacau gcgcaaaauu ugcauguagc      960

aaaaaaauga caggaaaaag uauucaacca gaaaacuuag aguauaggau aauguuaucg     1020

guacacggaa gucaacacuc aggaaugauu guaaacgaca cgggccacga aacugacgaa     1080

aauagagcaa aaguagaaau cacgccaaau ucaccacgag ccgaagccac auuaggagga     1140

uuuggaucac ugggauuaga uugcgaaccu agaacgggau uggauuuuag ugauuuauau     1200

uauuuaacua ugaauaauaa acauugguua guacauaaag agugguuuca ugauauaccu     1260

cuaccuuggc augccggggc agauacugga acuccacauu ggaauaauaa agaagcauua     1320

guagaauuua aagaugccca cgcaaaaaga caaacaguag uuguauuagg aagucaggaa     1380

ggagcuguac auacagccuu agcaggagca cuagaagcag aaauggaugg agcaaaagga     1440

agauuaucaa guggacacuu aaaauguaga uuaaaaaugg acaaacuaag acuuaaaggu     1500

gugucauacu ccuuauguac agccgcuuuu acuuuuacua aaauuccagc ggagacuuua     1560

caugggacug uaacaguaga aguucaguau gcagguacag augguccaug caaaguacca     1620

gcccaaaugg caguugauau gcaaacauua acaccaguag gaagauuaau cacagccaau     1680

cccguaauca cagaauccac agaaaauagu aagaugaugc uagaauuaga cccaccguuu     1740

ggagauaguu auauaguaau aggaguuggg gaaaagaaaa uaacacauca uuggcaccga     1800

agcgguagca ccauaggaaa agcuuuugaa gcaacaguua gaggagcuaa aagaauggca     1860

guauugggug auacagcaug ggauuucgga ucaguaggug gagcauuaaa cagcuugggu     1920

aaaggaauac aucaaauuuu cggagcagca uuuaaaagcu uauuuggagg aaugaguugg     1980

uucucacaaa uauuaauagg aacacuauua augugguuag gacuaaauac aaaaaaugga     2040

aguauaaguu uaauguguuu agcauuaggg ggaguacuaa uauucuuauc aacagcagua     2100

ucugcugacg uaggaugcuc aguagauuuu agcaaa                               2136


<210>  2584
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long CDS opt

<400>  2584
aggauaauaa augccagaaa agaaaagaaa aggagaggag cagacacaag cguaggaaua       60

guaggcuuau uauuaacaac agcaauggcc gcagagguaa cucgaagagg aucagcguac      120

uauauguauu uagauagaaa ugaugcagga gaagcaauau cauuuccaac aacauuagga      180

augaacaaau gcuacauaca aauaauggau uuagggcaua ugugugaugc cacuaugagu      240

uaugaaugcc caaugcuaga cgagggugug gaaccagaug acguagauug uugguguaac      300

acaacaucua cauggguugu auauggaacu ugucaucaca agaaaggaga agcacgaaga      360

agcaggaggg caguaacacu accaagccau ucaacuagaa aacuccaaac aagaagucaa      420

accugguuag aaucaagaga auauacgaaa caucuaauaa gaguagaaaa cuggauauuu      480

agaaaucccg gauuugcauu agcagcagca gcaaucgccu ggcuauuagg aaguucaaca      540

agucaaaaag ugauauaucu agucaugaua cuacuaauug caccagcuua uagcauaaga      600

uguaucggug ucagcaauag agacuucguu gaagguaugu cagguggaac guggguagac      660

guuguuuuag aacacggcgg auguguaaca guaauggcac aagauaaacc aacgguagac      720

auagaacuug ugacaacuac uguuaguaac auggcagaag uacgaagcua uugcuaugaa      780

gcaucuauau cagauauggc aucagauuca cgauguccua cacaaggaga agcauacuua      840

gauaaacaaa gcgauacaca auauguuugu aaaaggacau uaguagauag aggcugggga      900

aaugguugug gauuauuugg aaaagguuca uugguaacgu gugcaaaauu cgcuuguagc      960

aagaaaauga cagguaaauc aauacaaccu gaaaacuugg aauacagaau uaugcuauca     1020

guacauggau cacaacauuc cggaaugaua guaaaugaua cuggucauga aacagaugaa     1080

aaccgagcca aaguagaaau aacaccuaau ucuccaagag cagaagcuac auuaggagga     1140

uucggaucac uuggucuaga uugcgaacca agaacuggac uugacuuuag ugauuuauac     1200

uauuuaacaa ugaauaauaa acauuggcua guacauaaag aaugguuuca ugacauacca     1260

uuaccauggc acgcaggagc agauaccgga acaccacacu ggaacaauaa agaagcucua     1320

guagaguuca aagaugcuca ugcaaaaaga cagaccgugg uaguauuagg aucacaagaa     1380

ggagcuguac auacggcucu agcaggagca cuugaagcgg aaauggaugg ugcaaaagga     1440

cgauuaaguu cagggcaucu aaaauguaga uuaaaaaugg acaaauuaag auuaaaagga     1500

guaaguuaua guuuaugcac agccgcuuuu acauuuacua aaauaccagc agaaacguua     1560

cauggaaccg uuaccguaga aguacaauau gcaggaacag augguccaug caagguacca     1620

gcacaaaugg caguagauau gcaaacgcua acaccaguag gaagguuaau caccgcuaau     1680

ccagugauaa cagaaucgac cgaaaauucc aaaaugaugu uagaauugga uccaccuuuu     1740

ggagacuccu auauaguaau agggguuggg gaaaagaaaa uaacccacca uuggcauaga     1800

ucagggucaa caauaggaaa ggcuuucgag gcaaccgugc gaggagcaaa aagaauggcg     1860

guuuuagggg auacugcaug ggauuuugga agugugggag gagcauuaaa cagccugggu     1920

aaagguauac aucaaauauu cggagcagca uucaaaucau uauuuggagg aaugagcugg     1980

uuuagucaga uacuaauugg aacauuacug auguggcuug gauuaaacac caaaaacgga     2040

ucaauuagcu uaaugugcuu agcacuuggu ggaguauuaa uauuccuaag uacaguaguu     2100

ucagcagaug uagguugcuc aguagacuuc agcaaa                               2136


<210>  2585
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long CDS opt

<400>  2585
agaauuauaa augccagaaa agaaaaaaaa agaagaggcg ccgauacauc aguagggaua       60

guaggauugc uauuaacaac agccauggca gcagaaguua caagacgagg aucagcauau      120

uacauguacu uagauagaaa ugaugcagga gaagcuauaa guuucccgac uacuuuagga      180

augaacaagu guuauauuca aauuauggac uuaggacaca ugugugacgc aacaauguca      240

uaugaaugcc caauguuaga cgaaggagua gaaccagaug auguagauug uugguguaac      300

acuacaagcg cuugggucgu cuacgggaca ugccaccaca aaaaaggaga agcuagacga      360

aguagaagag caguaacccu accaucucau aguacacgaa aacuacaaac uagaccucaa      420

accugguuag aaucaagaga auacacaaaa cauuuaauac gaguagaaaa uuggauauuc      480

agaaacccag gauucgcguu ggcagcggcg gcaauugcau gguuauuagg aucaaguaca      540

ucucaaaaag ucauauauuu gguuaugaua cuuuuaauag cuccagcaua uagcauacga      600

uguauaggag uaagcaauag agacuuugug gaaggaaugu caggagggac auggguugac      660

guagucuuag aacauggagg auguguaacg gucauggcac aagauaaacc aacaguugau      720

auagaauuag uaacaacaac aguaucaaau auggcagaag uuagaucaua uuguuaugaa      780

gcaucaaucu cagacauggc aagugacuca agauguccca cgcaagguga agcauaucua      840

gacaaacaaa gugauacaca auauguaugc aaaagaacac uuguagacag aggauggggc      900

aacggaugcg gucucuuugg uaagggcagc uuaguuacau gugcaaaauu cgcguguagu      960

aaaaaaauga cagguaaauc aauacagccu gaaaaucuug aauaucgaau uauguuaucu     1020

guucacggau cacaacauuc uggcaugauu guaaaugaca caggucacga aacagaugaa     1080

aacagagcua aaguagaaau aacaccgaac ucaccaagag cagaagcuac uuuaggaggu     1140

uuugggaguu uaggacuaga uugcgaacca agaacaggac uugauuuuag ugauuuauac     1200

uauuuaacca ugaauaauaa acauuggcug guacauaaag aaugguuuca ugauauucca     1260

uuaccauggc augcaggagc ugauacagga acaccccacu ggaauaauaa agaagcgcuu     1320

guagaauuua aagacgcaca ugccaaaaga caaacaguag uaguuuuagg uagucaagaa     1380

ggggcagucc auacagcacu agcgggagca uuagaagcag aaauggaugg agcaaaagga     1440

cgauuaagcu caggacaucu aaaauguaga cugaaaaugg auaaauuaag gcuaaaagga     1500

guaagcuaua gucuuugcac agcagcauuc acuuuuacaa aaauuccagc ggaaacacua     1560

cauggaacug uaacaguaga agugcaauac gcaggaacug auggaccgug uaaaguucca     1620

gcacaaaugg caguagauau gcaaacauua acaccaguag gacguuuaau aacagccaau     1680

ccaguaauaa cagaaucaac cgaaaacucg aaaaugaugc uagaauuaga uccuccuuuu     1740

ggagauucgu acauaguuau aggugucgga gaaaaaaaga ucacacauca cuggcauaga     1800

agcggcagua caauaggaaa agcauuugaa gcuaccguac guggagcuaa aagaauggca     1860

guucuaggcg auacugcaug ggauuuuggc ucaguuggag gugcauuaaa cagcuuaggg     1920

aaaggaauac aucaaauuuu uggugcagca uucaaaagcu uguuuggagg aaugucaugg     1980

uuuagccaaa uauuaauugg aacuuuacuu augugguuag gacuaaauac aaaaaauggu     2040

aguauaucau uaaugugucu ugcauuaggu ggaguauuga uuuuuuuauc aacagcagua     2100

agcgcagaug uaggauguuc aguagacuuu uccaaa                               2136


<210>  2586
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long CDS opt

<400>  2586
agaauaauaa augcacgaaa agagaaaaag agacgaggcg cagacacaag uguaggaaua       60

guaggccuac uuuuaacuac agcaauggcu gcagaaguaa cgagacgagg uuccgcauau      120

uauauguacc uugacaggaa ugacgcaggg gaagcaauau ccuuuccaac cacauuagga      180

augaauaaau guuacauaca aauaauggau cuaggacaua ugugugaugc aacaaugagu      240

uaugaauguc caaugcugga ugaaggagua gaacccgaug acguagauug uuggugcaau      300

acuacaucaa cauggguagu auaugggacc ugucaucaua aaaaaggaga agcaagaagg      360

aguagaagag cagucacauu gccgagucac ucaacaagaa aauuacaaac aagaucccaa      420

acauggcuug aaucuagaga auacacaaaa caucuuauaa gaguugaaaa uuggauuuuc      480

agaaaccccg gauucgcgcu agcagcagca gcaauagcau ggcuccuugg aucauccaca      540

aguaaaaaag uaauauacuu agugaugaua uuacuaaucg caccagcaua caguauacgu      600

ugcauaggag uaucaaaucg agauuucguu gaagggaugu cuggagguac auggguagau      660

guuguacuag aacauggugg auguguuaca guaauggcgc aagacaaacc uacagucgau      720

auagaacuag uaacaacuac aguaagcaau auggcagaag uccgaucaua cuguuacgaa      780

gcaaguauau cugacauggc aagcgacagu cgauguccaa cacaaggaga agcauaucua      840

gauaaacaau cugacacaca auauguuugc aaaagaaccu uaguugauag aggauggggu      900

aaugggugug gcuuauuugg caaggguagu cuuguaaccu gugcaaaauu cgcauguagu      960

aaaaaaauga caggaaaaag cauacaacca gaaaaucuag aauauagaau aauguuauca     1020

guacauggua gucaacauuc aggaaugaua guuaaugaca ccggacacga aacugaugaa     1080

aaucgagcaa aaguagaaau aacaccaaau aguccacgag cagaagcuac auuaggcgga     1140

uuuggaucgu uaggauuaga uugugaacca agaacuggau uagauuuuag ugaucuauau     1200

uaccuaacaa ugaauaauaa acacugguua guacauaaag aaugguuuca cgacauacca     1260

cuacccuggc acgcuggagc cgauacugga acaccacauu ggaacaacaa agaagcacua     1320

guagaguuua aagaugcaca cgcaaaaaga caaacaguag uaguauuagg aucacaagaa     1380

ggcgcagucc auacagcacu agcaggagca uuagaagcgg agauggacgg agcaaaagga     1440

agacuaaguu caggccauuu aaaaugcagg cuaaaaaugg auaaauuaag auuaaaagga     1500

guaucauauu cacuauguac cgccgcauuu acauuuacaa aaauaccagc agaaacuuua     1560

cauggcaccg uuacugucga aguccaauau gcuggaacag auggaccaug uaagguucca     1620

gcucaaaugg caguggauau gcaaacacua acuccaguag guagacuaau aacagcaaau     1680

ccgguaauua cugaaucaac agaaaacuca aaaaugaugc uagaauuaga uccuccguuc     1740

ggagacucgu acauaguuau agguguaggu gaaaaaaaaa uaacacauca uuggcauaga     1800

ucaggaucaa ccauagggaa agcuuuugag gcuacgguaa gaggagcaaa acgaauggcc     1860

guuuuaggag auacugcaug ggauuuugga agcguaggag gagcauuaaa cucauuagga     1920

aaaggaauac aucaaauauu uggagcagca uuuaaaaguc uauuuggagg aaugucaugg     1980

uuuucacaaa uacuaauagg aacucuguua augugguuag gacuaaauac aaaaaaugga     2040

ucuauaagcc uuaugugccu agcucuagga ggaguuuuaa uauucuuaag uacagcugua     2100

ucagcagaug uuggauguuc uguagacuuc ucuaaa                               2136


<210>  2587
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long CDS opt

<400>  2587
agaauuauaa augcacgaaa agaaaagaaa agaagaggcg cugauacaag cgugggaauu       60

gugggauuau uauuaacaac agcaauggca gcggaaguua caagaagagg aagugccuau      120

uauauguauc uagauagaaa ugacgcagga gaggcaaucu cauuuccaac uacacuugga      180

augaauaaau guuacaucca aauaauggac uuaggacaua ugugcgaugc aacuauguca      240

uacgaauguc cgauguuaga ugaaggagua gagccagacg auguagauug cugguguaau      300

acaacuucaa cauggguagu auacggcacg ugucaucaua agaaaggcga agcuagacga      360

uccaggagag caguaacauu accuucacau aguacaagaa aauuacagac aagaucacaa      420

acaugguuag aaucuagaga auauacaaaa caucuaauua gaguugaaaa cuggauauuu      480

agaaacccag gauucgcacu agcagcugca gcaauagcau gguuacuagg cagcucuacg      540

agacaaaaag uaauauaccu uguuaugaua cuccuaauag caccagccua uucaauuaga      600

ugcauaggag uaaguaacag agauuuuguu gaaggaauga gcggagguac auggguagau      660

guaguauuag aacauggagg augugugaca guaauggcac aggauaaacc aacuguagac      720

auagaauuag uaacaacaac uguaagcaau auggcagaag uaagaaguua cuguuaugaa      780

gccucaauca gcgauauggc aagugauuca agauguccaa cacaagguga ggcuuauuua      840

gauaaacaaa gcgacacaca auauguaugc aaaaggacau uaguagacag aggaugggga      900

aacgguugcg gccuauucgg aaaaggaagu cuaguuacau gugcaaaauu ugcuuguucu      960

aagaaaauga caggaaaaag cauacagccc gaaaaccuag aauaucgcau aauguuaagc     1020

guacauggau cacaacauag uggaaugaua guuaaugaua caggacacga aacagaugaa     1080

aacagagcaa aaguggaaau aacuccaaau ucacccagag cagaagcaac acuaggagga     1140

uucggaucac uaggacuaga uugugaaccg aggaccggau uggauuuuag ugauuuguac     1200

uauuugacua ugaauaacaa acauuggcua guacacaaag aaugguuuca ugauauacca     1260

uuaccauggc augcaggagc agauacgggu acaccacauu ggaauaacaa agaagcauua     1320

guagaauuua aagacgcaca ugcaaaacga caaacaguag uaguacuugg gagucaggaa     1380

ggagcaguac auacagcauu agcaggagca uuagaagcag aaauggaugg ugcaaaaggu     1440

agacuuucau ccggacaccu gaaaugucga cuaaaaaugg auaaauuaag auuaaaaggg     1500

gugagcuaca gcuuguguac ugcagcauuu acuuuuacua aaauaccagc ugaaacacuu     1560

cauggaacag uaacuguaga agugcaguau gcaggaacag augguccaug uaaaguacca     1620

gcacagaugg cuguagauau gcaaacucua acaccuguug gcaggcuaau aacugcaaau     1680

ccaguaauaa cagaaucaac agaaaacuca aaaaugaugu uggaauuaga uccaccauuu     1740

ggagauaguu acauaguaau agguguuggg gaaaagaaaa uuacucauca uuggcacaga     1800

ucaggaucua cgauaggaaa agcauuugaa gcuacuguaa gaggcgcaaa aaggauggca     1860

guauuaggug auacggcaug ggacuuugga ucuguaggug gagcguugaa uagucuaggu     1920

aaaggaauac aucaaauauu uggagcagca uuuaaaucau uauuuggugg caugaguugg     1980

uuuagucaga uacuuauagg aacucuauua augugguuag gauuaaacac caaaaaugga     2040

ucaauaucac uuauguguuu agcauuaggg ggaguccuaa ucuuccuauc aacagcagua     2100

agcgcggaug uaggauguuc aguagauuuu agcaaa                               2136


<210>  2588
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long CDS opt

<400>  2588
cguauuauaa augcaagaaa agaaaagaaa agaagaucag cagauacauc aguugguauu       60

gugggcuuac uucucacaac agcgauggca gccgaaguaa cuagaagagg aucagcuuac      120

uacauguauu uagacaggaa ugacgcuggg gaagcuauau cauuuccuac aacauuagga      180

augaauaaau gcuauauaca aauaauggac cuuggacaca ugugugaugc aaccaugagc      240

uaugaauguc caauguuaga cgaaggagua gagccagaug auguagacug uuggugcaau      300

acuacuucca ccuggguggu auauggaaca ugccaccaua agaaagguga agcaagaaga      360

aguagacgag caguaacacu gccaucacac aguacaagaa aacuucaaac aagaagccaa      420

acauggcuag aaaguagaga auauacaaaa cauuuaauaa gaguagaaaa cuggauauuu      480

agaaauccug gauuugcuuu agcugcugca gcaauagcau ggcuauuggg aagcaguaca      540

agccaaaaag ucauauaccu aguaaugaua cuucuuauag caccagcaua uucaauaaga      600

uguauaggag uaucaaauag agauuuuguc gaaggcauga guggaggaac auggguagau      660

guuguuuuag aacauggagg auguguaaca guaauggcac aagauaaacc aacaguagac      720

auagaauuag uaacaacaac agucucaaac auggcagaag uaagaucaua uuguuacgaa      780

gcaucaauuu cagauauggc uucagauuca agauguccua cacaaggaga agccuaccua      840

gauaaacaaa gugacacuca auacguaugc aaaagaacuc uaguagauag agguugggga      900

aacggaugug gguuauuugg aaaaggaagc cucguaacau gugcaaaauu cgccugcagu      960

aagaaaauga caggaaaaag cauacaacca gaaaacuuag aauauagaau aaugcuuagu     1020

guacacgguu cucaacauuc aggaaugaua guaaacgaua caggccauga aacagaugaa     1080

aacagagcga aaguagaaau aacaccaaau ucacccagag cagaagccac acuuggagga     1140

uuuggcucau uaggauuaga uugugaacca cgaacaggac uagacuuuag ugaucuauau     1200

uacuuaacca ugaauaacaa acacuggcua guacauaaag agugguuuca cgauauucca     1260

cucccauggc acgcaggagc agauacugga acaccgcauu ggaauaauaa agaagcccuc     1320

guagaauuua aagaugcaca ugcuaaaagg caaacggugg uaguauuagg aagucaagaa     1380

ggagccguac acacagcauu ggcaggggca cuagaggcug aaauggacgg agcaaaaggu     1440

cgauuaucaa guggacauuu aaaauguaga uuaaaaaugg auaaauuaag gcuaaaagga     1500

guaucguauu cauuaugcac agcagcauuu acauuuacaa aaauuccggc ggaaacacua     1560

cauggaacag uaacaguaga aguacaauau gcgggaacag acgguccaug uaaaguaccu     1620

gcacaaaugg caguagauac acaaacuuug acaccaguag gaagacuaau uacagcaaau     1680

ccaguaauaa cugaaucaac agaaaauuca aaaaugaugu uagaauuaga uccaccuuuc     1740

ggagauaguu acauaguaau uggagugggg gaaaagaaaa uaacccacca cuggcaccga     1800

agcgguucaa cuauaggaaa agcauuugag gcaacaguua gaggcgcaaa aagaauggcg     1860

guauuaggag acacagcaug ggauuucgga ucuguaggag gggccuuaaa cucacuagga     1920

aaaggaauac aucaaauauu uggagcagcg uuuaaaucac uauuuggugg gaugucuugg     1980

uuuagccaaa uacuuauagg cacgcuacua auguggcucg guuuaaauac caaaaaugga     2040

ucuauaaguu uaaugugucu agcauuagga ggaguacuaa uauuuuugag uacagcagug     2100

ucagcugaug uaggcuguuc aguagauuuu ucaaaa                               2136


<210>  2589
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long CDS opt

<400>  2589
agaauuauaa augcaagaaa agaaaaaaaa agacgaggag cagacacuaa uguagguauc       60

guaggacuau uauuaacgac agccauggca gcugaaguaa cccgaagggg aucggccuau      120

uacauguauu uagauagaaa ugaugcagga gaagcaauaa guuuuccaac aacguuaggu      180

augaauaaau guuacauaca aauaauggau uuaggucaua ugugugacgc aacaaugagu      240

uacgaaugcc caauguuaga ugaaggugug gaacccgaug acguugauug cuggugcaau      300

acaaccucaa cauggguugu cuaugggaca ugucaucaua aaaaaggaga agcaagaaga      360

aguagaaggg ccguuacucu uccaagccau agcacaagaa aacuccaaac uagaucacaa      420

acauggcuag aaaguagaga auacaccaaa cacuuaauaa gaguagaaaa uuggauauuu      480

agaaauccag gauuugcauu agcagcagca gcuauagcau ggcuauuagg aucgucuaca      540

agucaaaaag uaauauauuu aguuaugaua cuacuaauag caccagcaua cagcauaagg      600

ugcauaggcg uaucaaacag agauuuuguu gaaggaaugu caggaggaac cuggguagau      660

guaguguuag aacauggagg gugcgugaca guaauggccc aagauaaacc aacaguugau      720

auagaacuug ucacgacaac agugucaaac auggccgaag uaagauccua uuguuacgaa      780

gcaucaauau cggauauggc auccgauagc agauguccua cacaaggaga agcauauuua      840

gacaaacaaa gcgauacaca auauguaugu aaacgaacau uaguugacag aggguggggu      900

aaugggugug gacuauucgg uaaagguagu uuagucacuu gcgcaaaauu ugcauguucu      960

aagaaaauga caggaaaauc aauacaaccg gaaaaccuag aauacagaau aaugcuauca     1020

gugcauggau cacaacauag cggaaugaua guaaaugaca cuggucacga gacagaugaa     1080

aauagagcaa aaguagaaau aacaccaaau ucaccaagag cggaagcaac auuaggaggu     1140

uuuggaucau ugggauuaga uugcgaacca agaacaggau uagauuuuuc agaucuuuau     1200

uaucugacaa ugaauaauaa acauuggcua gugcacaaag aaugguucca ugauauaccc     1260

cuaccauggc augcaggugc cgauaccgga acaccacauu ggaauaauaa agaagcauua     1320

guugaauuua aagaugcuca cgccaaaaga caaacaguag uaguauuagg cucacaagaa     1380

ggggcaguac auaccgcauu ggcuggagcg uuagaagccg aaauggacgg ugccaaagga     1440

agacucucaa guggucauuu gaaaugcaga cuaaaaaugg auaaauuaag auuaaaagga     1500

guaucauaua guuuaugcac agcugcauuu acauuuacaa aaauaccagc agaaacucua     1560

cauggaacag uuacaguuga gguucaauau gcaggaacag auggaccaug caaagucccu     1620

gcacaaaugg caguagacau gcaaacacug acaccuguag gaagauuaau aacugcaaau     1680

ccgguaauaa cagaaaguac cgaaaacucg aaaaugaugu uagaacuuga uccuccguuc     1740

ggagacagcu acauuguaau aggaguagga gaaaaaaaaa uaacgcauca cuggcauaga     1800

ucaggaucga ccaucggaaa agcauuugag gccacuguga ggggagcaag aagaauggca     1860

guauuaggcg auacagcuug ggauuuuggu ucagucggag gagcauugaa uaguuuggga     1920

aaaggaauac aucaaauauu uggagcagca uuuaaaucau uauuuggugg uaugagcugg     1980

uucucccaga uauuaauagg aacacuauua augugguuag guuuaaauac uaaaaaugga     2040

aguauaucau uaauguguuu agcuuuagga ggcguacuaa uauuuuuauc aacggcuguu     2100

ucagcagaug ugggaugcuc aguagacuuc ucaaaa                               2136


<210>  2590
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long CDS opt

<400>  2590
aggauaauua augcuagaaa agaaaagaaa agaagaggag cagaaacaag uguaggaauc       60

guugguuuau uauuaacaac ugcuauggca gccgaaguaa caaggagagg gagugccuau      120

uacauguauu uagacagaaa ugacgcagga gaagcaauuu ccuucccaac uacuuuaggu      180

augaacaaau guuauauaca aauaauggau uugggacaua ugugcgaugc gacaaugagu      240

uaugaauguc caaugcuaga cgaaggaguc gaaccugaug acguagacug uugguguaac      300

accacaagua cauggguagu auauggaacg ugccaucaua agaaaggaga agcgagaaga      360

aguaggagag ccguaacauu accaagucac agcacaagaa aauuacaaac aagaagucaa      420

acaugguuag aaucaagaga auauacaaaa caccuaauaa ggguagaaaa cuggauauuu      480

agaaauccag gauuugcauu ggcagcagca gccauagcau gguuguuagg aucaaguaca      540

agucaaaaag uaauauauuu aguaaugaua uuauuaauag caccagcaua uucaauaaga      600

ugcauaggug uaucaaauag agacuuugua gaagguauga guggaggaac uuggguagau      660

guaguuuuag aacacggagg augcguaaca guaauggcuc aagacaaacc aacaguagau      720

auagaauuag ucacuacaac aguaucaaau auggcagaag ucagaagcua uuguuacgaa      780

gcaagcauuu cagacauggc aucugauuca cguuguccua cgcaaggaga agccuauuua      840

gauaaacaau ccgacacaca auauguaugu aaaagaaccc uaguagacag agguuggggu      900

aauggaugug gccuauucgg gaaaggaagu uuagucacau gugcuaaauu ugcaugcagu      960

aagaaaauga caggaaaauc aauacaaccg gaaaaucuag aauacagaau aaugcuaagu     1020

guacauggaa gucaacauuc cggcaugaua gugaaugaua cugggcauga aaccgaugaa     1080

aacagagcaa aaguagaaau aacaccaaau ucuccgcgug cugaagcuac acuaggggga     1140

uuuggaucau uaggucuuga cugugaacca aggacugguu uagauuucuc ggaucuauau     1200

uaucuaacua ugaauaacaa acauuggcuu guacauaaag aaugguuuca cgauauacca     1260

cuaccauggc augcaggagc agauacaggg acuccucacu ggaacaauaa agaagcauua     1320

guugaguuua aagaugcaca ugcaaaaaga caaacuguag uuguauuagg cucacaagaa     1380

ggugcaguac acacagcucu agcaggggcu cuagaggcgg aaauggaugg ugcaaaagga     1440

agacuuucaa guggacacuu aaaaugcaga cuaaaaaugg auaaauugag auuaaaagga     1500

guaaguuauu cuuuauguac agcagcauuc acauuuacaa aaauaccagc agaaacauua     1560

cauggaacag uaacaguuga aguacaauac gcgggaacag augggccaug caaaguccca     1620

gcgcaaaugg cuguagacau gcaaacgcug acacccguag ggcgacuaau uacugcaaau     1680

ccaguaauaa cugaaaguac agaaaacagu aaaaugaugc uggaauuaga ucccccguuu     1740

ggagacagcu auauaguaau aggaguaggu gaaaagaaaa ucacacauca uuggcauaga     1800

ucagguucaa caauaggaaa agccuuugaa gcaacaguua gaggagccaa acggauggca     1860

gugcuuggag auacggcuug ggacuucgga ucaguaggag gcgcauuaaa caguuuaggg     1920

aaaggaauac accaaauuuu cggugcagcu uuuaaaaguu uauuuggagg aaugucaugg     1980

uuuucucaaa uauuaauagg aacuuugcua auguggcuag gauuaaacac caaaaaugga     2040

ucaauaucac ugaugugucu agcucuaggu gguguauuaa uauuucuauc aacagcaguu     2100

ucagcagaug uuggaugcuc aguugauuuu ucgaaa                               2136


<210>  2591
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long CDS opt

<400>  2591
agaauaauaa augccagaaa agaaaagaaa agaagaggug cagauacauc cguaggaaua       60

guaggauuac uuuuaacaac agcaauggca gcagaaguaa caagaagggg uucagcauau      120

uauauguacc uagauaggaa ugacgcggga gaagcuauau cauuucccac aacccuagga      180

augaauaaau gcuauauaca gaucauggac cuuggacaua ugugugaugc aacaauguca      240

uacgaauguc caauguuaga ugaaggagua gaaccggaug acgucgauug cuggugcaac      300

acuacgagua ccuggguagu cuauggaaca ugucaucaua aaaagggaga agcaagacga      360

aguaggagag cagucacuuu gccaagucac aguacuagaa aacugcagac aagaagucaa      420

acaugguuag aaucaagaga auauacuaag caccuaauaa gaguugagaa uuggauauuu      480

agaaacccag guuuugcucu ugcagcugcg gcuauagcgu ggcuguuagg aucaucaaca      540

agucaaaaag uuauauacuu agugaugaua uuauuaauug cuccugcaua uucacuaaga      600

uguauuggag uaaguaauag agauuuugua gaagguauga guggaggcac guggguugau      660

guaguacuag aacauggcgg auguguuaca guaauggcac aagauaaacc uacaguagau      720

auagaauuag uaacuacuac aguaaguaau auggcagaag uuagaaguua cuguuaugaa      780

gcaaguauau cagauauggc aagugauuca agauguccca cgcaaggaga ggcuuaucua      840

gauaaacaau cagauacaca auacgucugu aaacgaaccu uaguagaucg agguugggga      900

aaugggugcg guuuauucgg aaaaggauca uuaguaacuu gugcaaaauu ugcauguuca      960

aagaaaauga caggaaaaag cauccaacca gaaaacuuag aauauagaau aauguuaagu     1020

guacaugguu cucaacauuc aggaaugaua guaaacgaca caggccauga aacagaugaa     1080

aauagagcaa aaguagaaau aacaccaaau ucaccaagag cugaagcgac uuuaggagga     1140

uuugguagcu uagguuuaga uugugaaccc agaacaggau uggauuucag ugauuuauau     1200

uaucuaacga ugaauaacaa acauugguua guacauaaag aaugguucca ugauauacca     1260

uugccuuggc augcuggagc agauacagga acgccacacu ggaauaauaa agaagcauua     1320

guagaauuua aagaugcaca cgcgaaaaga cagacaguag ucguacuagg aucacaagaa     1380

ggagcaguac auacagcauu ggccggagcu uuagaagccg aaauggaugg agcaaaaggc     1440

agauuaagca guggccacuu aaaaugucga uuaaaaaugg auaaauuaag auuaaaagga     1500

gucaguuaca gcuuauguac agcugcauuc acauuuacca aaaucccagc agaaacauua     1560

caugguacug ugacaguaga aguacaauac gcuggaacag auggcccuug uaaaguacca     1620

gcacaaaugg caguagauau gcaaacauua acaccgguag gaagguuaau uacugcaaau     1680

ccaguaauaa cugaaucaac agaaaauagu aaaaugaugc uagaauuaga cccacccuuu     1740

ggggauaguu auauagugau aggaguagga gaaaagaaaa uaacacacca cuggcaucgu     1800

agcggaagua caauaggaaa agcauuugaa gcuacgguaa gaggggcaaa acgaauggcu     1860

gugcuagggg acacagccug ggauuucgga ucagugggag gugcauuaaa uucauuagga     1920

aaagggauac aucaaauauu cggagcugcg uuuaaaucau uauucggagg aaugaguugg     1980

uuuucucaaa uauuaauugg aacgcuucua auguggcuag gacuaaauac uaaaaacggu     2040

ucaauaucuc ugauguguuu agcauuggga ggaguacuaa uauuccuuag uacagcagua     2100

agugcggaug uaggcugcuc aguagauuuu aguaaa                               2136


<210>  2592
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long CDS opt

<400>  2592
agaauuauaa acgcaagaaa agaaaaaaag agaagaggag cugacacuaa uguaggaaua       60

guaggacugu uauuaacaac agcgauggca gcagaaguaa cgagaagagg aucugcauac      120

uauauguauu uagauaggaa ugaugcagga gaagccauau cauuuccgac aacauuggga      180

augaauaaau guuacauaca aauaauggau cuaggacaua ugugugacgc aacaaugagu      240

uacgaguguc ccauguuaga ugaagggguu gagccagacg auguggauug uugguguaac      300

acaacaagua cuuggguugu auauggaacu ugccaccaua aaaaagguga agcuagaaga      360

ucacguaggg cgguaacacu accaucucac aguacccgua aacuacaaac gagaagccag      420

acaugguuag aaagcagaga auauacaaaa cauuuaauaa gagucgaaaa uuggauguuu      480

agaaauccag gcuuugcauu agcagcagcc gcuauagcau ggcuucuugg aucaaguaca      540

ucacaaaaag uuauauauuu aguaaugaua uuguuaauag ccccagcuua cucaauaaga      600

uguauuggag uaagcaacag agauuuugua gaaggaauga guggaggaac auggguugau      660

guaguacuag agcauggugg gugcguaaca gucauggcgc aagacaaacc uacaguagau      720

auagaauuag uaacaacaac aguaaguaau auggcagaag ugcgaucaua uuguuacgaa      780

gcaagcauuu cugauauggc aucagauagu cguuguccaa cacaaggaga agcauaccua      840

gauaaacaau cagauaccca auacguaugu aaaagaacuu uaguagaucg agguuggggu      900

aauggaugug gacuauuugg uaaaggaagc cuaguuacuu gugcuaaguu ugcaugcagu      960

aaaaaaauga cagguaaauc aauacaacca gaaaaucuag aguauagaau aauguuaagu     1020

guacauggaa gucaacauag ugguaugauu guaaaugaua caggacacga aacagacgaa     1080

aaccgagcua aaguagaaau cacgccaaau agucccaggg cagaagcaac auuaggagga     1140

uuuggcucgu uaggauuaga cugugaacca cgaacugggc uagauuuuag cgauuuauau     1200

uaucuaacaa ugaacaacaa acacugguua guacauaaag aaugguuuca ugauauccca     1260

cuaccuuggc augccggagc agacacagga acaccacacu ggaauaauaa agaagcacua     1320

guagaguuua aagacgcaca ugcgaaaagg caaacaguag uaguacuagg aucacaagaa     1380

ggagcgguac auacggcacu agcuggagcc cuagaagcag agauggacgg cgcuaaagga     1440

agauugagca guggacaccu aaaaugucgu uugaaaaugg auaaauuaag acucaaagga     1500

guaaguuauu cguuaugcac agcugcguuu acauuuacca aaauaccagc agaaacgcua     1560

cauggaaccg uaacggugga aguucaauau gcaggaacag auggaccaug caaaguacca     1620

gcucagaugg cgguagacau gcaaacauua acaccaguag ggagguuaau aacagcaaac     1680

ccugugauaa cugaaaguac agaaaauagu aagaugaugc uagaacuaga uccaccauuc     1740

ggagauucau auauaguaau aggaguaggg gaaaaaaaaa uaacucauca uuggcauaga     1800

agugguagua caaucggaaa agcauuugaa gcaacaguac gcggggcaaa aagaauggca     1860

guacuuggag auacagcaug ggauuuugga aguguaggag gugcguuaaa uaguuuagga     1920

aaaggaauac aucaaauauu cggggcagca uucaaaaguu uauuuggagg aaugaguugg     1980

uucagucaaa uauuaauagg aacauuguua auguggcuag gucuuaauac aaaaaauggu     2040

ucaauaaguu uaaugugucu ugcauuagga ggagucuuaa uauuuuuaag cacagcagua     2100

agugcggacg uuggguguuc aguugacuuu aguaaa                               2136


<210>  2593
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long CDS opt

<400>  2593
agaauaauaa augcacgcaa agagaaaaaa agaagaggag cagauacaag uguaggaaua       60

guugguuuau uacuaacaac agcaauggca gcugaaguca caagaagagg aucagcauau      120

uauauguacc uagacagaaa cgaugcugga gaagcgauau cauuuccuac gacacuuggu      180

augaauaaau gcuauauuca aauaauggac uugggacaua ugugugaugc aaccauguca      240

uacgaaugcc cuauguuaga ugagggugua gaaccugacg auguagauug cugguguaac      300

acaacuagua cuuggguagu uuauggaaca ugucaccaca aaaagggaga agcaagaaga      360

ucaagaaggg ccguaacucu cccaucacac aguacaagaa aauuacaaac aagaucacaa      420

acgugguuag aaucaagaga auauacaaaa cacuuaauaa gaguugaaaa uuggauacua      480

cguaauccag gauucgcacu cgcagcugcg gcuauagcau ggcucuuagg uucgaguacc      540

ucucagaagg uaauauacuu aguaaugaua uuauuaauag caccagcuua uucuauaaga      600

uguauaggag ucaguaauag agauuuuguu gaaggaaugu caggaggaac uuggguagac      660

gugguacugg aacacggagg augcgugacc guuauggcgc aagauaaacc aacgguagau      720

auagaauuag uaacaaccac aguuaguaau auggccgagg uaaggucuua uuguuaugaa      780

gccucuauau cugacauggc aucagacuca agaugcccua cacaaggaga agcauauuua      840

gauaaacaau cagauacaca auauguaugu aaacgaacac uaguagauag gggcuggggu      900

aauggaugcg gacuauucgg aaaaggaagu uuagucacau gugcaaaauu cgcauguucu      960

aaaaaaauga caggaaaauc aauacaacca gaaaauuuag aauaucgaau uauguuauca     1020

guacauggau cacaacauuc uggaaugaua guaaacgaca caggccacga aacagacgaa     1080

aauagagcaa aaguugaaau aacaccaaau aguccucgag ccgaagcaac acuuggaggu     1140

uuuggaaguc uaggauuaga uugugaacca agaacaggau uagacuucuc ggaucuauau     1200

uaucugacca ugaauaauaa acauugguua gugcacaaag aaugguuuca ugauauaccg     1260

uuaccauggc augccggagc agauacaggc acaccacauu ggaauaauaa agaagcacua     1320

guagaauuca aagaugcuca cgcuaaaaga caaacagugg uaguauuagg uagccaagaa     1380

ggagcaguac acacagcccu agcaggugcu cuugaagcag aaauggaugg agcaaaggga     1440

agauuaaguu ccggacaccu gaaaugcaga cuaaaaaugg auaaauuaag acuaaaagga     1500

gucucauauu cauugugcac agcagcauuu acuuuuacaa aaauuccagc agaaacguug     1560

cacggaacag uaacuguaga aguacaauac gcaggaacag auggaccgug uaaaguacca     1620

gcacaaaugg caguugauau gcaaaccuua acaccaguug gaagauuaau aacugcaaac     1680

ccaguaauaa cagaaaguac ggaaaauuca aaaaugaugu uagaauuaga uccuccauuc     1740

ggagauucau acaucguaau aggaauagga gaaaaaaaaa uaacacauca uuggcacaga     1800

ucgggaucaa caauagguaa agcauuugaa gcuacaguaa gaggagcaaa acggauggca     1860

guauuaggag acacugccug ggauuuugga ucaguagggg gagcauuaaa uucacuagga     1920

aaaggcaucc aucaaauauu uggugcugcu uuuaaauccc uauucggagg aaugagcugg     1980

uucucacaaa uacuuauugg aacucugcua auguggcuag gauuaaauac aaaaaaugga     2040

agcauaagcu uaaugugucu agcucuagga ggcguucuaa uuuuuuuaag uacagccgua     2100

ucagcugacg uaggauguuc agucgauuuc ucaaaa                               2136


<210>  2594
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long CDS opt

<400>  2594
agaauaauua acgcuagaaa agaaaagaaa agaagaggag cugacacaag cgugggaaua       60

guaggacucu ugcuaacaac agcaauggcu gcagaaguaa caagaagagg aagugcauau      120

uauauguauu uggaucgaaa ugacgcagga gaagcaauau cauuuccaac aacacucgga      180

augaauaaau gcuauauaca aauaauggau cucggacaua ugugcgacgc cacaaugucu      240

uaugaauguc cuaugcuaga ugaaggagua gaaccagacg augucgauug uugguguaau      300

acaacaagca cauggguggu auacggaaca ugucaucaua agaaagguga agcaaggaga      360

aguagacggg caguaacacu accaucacau aguacgcgaa aacuacaaac aagaucacaa      420

acuuggcuag aaucaagaga auacacaaaa caucuaauua gaguagaaaa cuggauauuu      480

agaaacccag gcuucgcacu ggcagcagca gcaauagcau gguugcuagg gucaagcaca      540

ucacaaaaag uuauauauuu aguaaugaua uuauugauag cuccagcaua caguauaaga      600

uguauaggag ugucaaaucg agauuuuguu gaaggaaugu caggaggcac uuggguagau      660

guaguauuag aacauggugg uugcguaaca guaauggcuc aagauaaacc aacaguagau      720

aucgaauuag uaucaacaac aguuucaaac auggcagaag uaagaucuua uuguuacgaa      780

gcaaccauaa gcgauauagc aagugauuca agguguccua cacaaggaga agcauaucua      840

gacaaacaau cagauacaca auacguaugc aaaagaacau uaguugauag aggaugggga      900

aauggaugcg gguuauuugg aaaagggagu uuaguaacuu gugcaaaauu ugcguguuca      960

aagaaaauga caggaaaauc aauacaacca gaaaacuuag aauaucgaau uauguuguca     1020

guacauggau cacaacauag cgggaugaua gucaaugaua caggacauga aacugacgaa     1080

aauagagcaa aaguagaaau aacaccaaau agcccuagag ccgaagcuac auuaggagga     1140

uuugguucac ucggacuaga cugugaacca aggacugguc uugauuuuuc agauuuauau     1200

uacuuaacaa ugaauaauaa acacugguua guccacaaag aaugguuuca ugacauacca     1260

uugccuuggc augcaggagc agauaccgga acaccacacu ggaauaauaa agaagcauug     1320

guagaauuua aagacgcuca ugcaaaaagg caaacagcug uuguacucgg aucucaagaa     1380

ggagcaguuc acacagcuuu agcuggagca cuugaagcag aaauggaugg agcaaaagga     1440

agacuaucuu cgggccauuu aaaaugcaga cuaaaaaugg auaaauugcg auuaaaaggg     1500

guuaguuaca guuuauguac agcggcuuuu acuuuuacaa aaauaccagc agaaacacua     1560

cauggaacag uaacaguaga aguacaauau gcggguacag auggcccaug caaaguacca     1620

gcacaaaugg caguagauau gcaaacacua acgccaguag gaaggcugau uacagcaaau     1680

ccaguaauaa cagaaucuac ugaaaacuca aaaaugaugc ucgaacuaga uccaccauuu     1740

ggggauucuu auaucguuau aggcguaggc gaaaagaaaa uuacacacca uuggcacaga     1800

ucaggaucaa caauaggaaa agccuuugaa gcuacaguaa gaggugcaaa aagaauggcu     1860

guucuuggag acacagcaug ggauuuuggu aguguagggg gagcauugaa cucacuaggg     1920

aaaggcauac aucaaauuuu uggugcagca uuuaaaucau uauucggagg aaugagcugg     1980

uuuagccaaa uucuaauugg aacuuuacug auguggcuag gccuuaauac uaaaaaugga     2040

aguauaucac uuaugugcuu agcauuagga ggaguacuga uauuuuuauc aacagccguu     2100

ucagcagacg uugguugcag uguugauuuu aguaaa                               2136


<210>  2595
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long CDS opt

<400>  2595
agaauaauaa acgcucguaa agaaaaaaag agaagaggag cagacacaag uguaggcaua       60

guaggauugc uuuugacuac agcaauggca gcagagguaa caaggagagg gucagcuuau      120

uauauguacu uagacagaaa cgacgcaggu gaagccauau ccuuuccgac aacacuaggu      180

augaauaaau gcuacauaca aaucauggau uuaggacaua ugugcgaugc aacaaugagc      240

uacgaauguc caauguuaga ugaaggagua gaaccagaug auguugauug uugguguaac      300

acaacuagca cauggguagu cuauggcaca ugucaccaca aaaaaggcga agcuagaaga      360

uccagacgag ccguaacauu accuagucau agcacgagaa agcuacaaac aagaagccaa      420

acaugguuag aaaguagaga auauacaaaa caucuaauca gaguggaaaa cuggauauuc      480

agaaaccccg guuuugcauu agcugcagca gccauagcau ggcuauuagg aaguucaacg      540

agccaaaaag uaauauauuu aguaaugaua uuacugaucg cuccagcaua uucaauacga      600

uguauagggg uaagcaauag agacuucguc gaaggaaugu caggaggaac auggguagau      660

guugugcucg aacacggugg uugugucaca guaauggcac aagauaaacc aaccgucgau      720

auagaacuag uaacaacaac aguuucaaau auggcagaag uaagaucuua cuguuaugag      780

gcaaguauaa gugauauggc gucagauuca agaugcccaa cucaaggaga agcauauuua      840

gauaaacaaa gugauacaca auacguaugu aaaagaacuu uaguagauag aggaugggga      900

aauggcugug gucuauuugg caaaggaagu cuaguaacuu gugcaaaauu cgcaugcagc      960

aaaaaaauga caggaaaaag cauacaaccc gaaaacuuag aauaucgaau aaugcuguca     1020

guccauggaa gucaacacag ugguaugaua guuaaugaua cuggacauga aacagacgaa     1080

aauagagcga aaguagagau aacuccaaau ucgcccagag cagaagcuac ccuaggaggu     1140

uuuggaucac ucgguuugga uugcgaaccu cgaacagguu uagacuuuag cgacuuauau     1200

uaccuaacaa ugaacaauaa acauuggcua guacacaaag aaugguuuca ugauauaccu     1260

uuaccauggc augcuggcgc agauacagga acaccgcauu ggaauaauaa agaagcacua     1320

guagaauuua aagaugcaca ugccaaaaga caaacugucg uuguauuagg uacacaagaa     1380

ggagcuguac auacagcauu agccggagca uuagaagcag agauggacgg ugcaaaaggc     1440

cgauuaaguu cagggcaucu aaaauguaga uugaaaaugg acaaauuacg acuaaaagga     1500

guuucauauu cauuauguac cgcagcauuc acuuuuacaa aaauaccugc agagacauua     1560

caugguacag uaacaguaga aguacaauau gcaggaacag acggaccaug uaaaguaccu     1620

gcucaaaugg caguagauau gcagacucua acaccagucg gcagauuaau aacagcaaau     1680

cccguaauaa cagaaucgac ggaaaauucu aaaaugaugu uagaacucga uccuccauuu     1740

ggagauaguu acauaguaau ugggguagga gaaaaaaaaa ucacucauca uuggcauaga     1800

aguggaucga cgauaggaaa agcauucgaa gcaacaguac gaggagcuaa aagaauggcu     1860

guuuuaggug acacugcaug ggacuuugga ucaguuggag gagcguuaaa uagcuuagga     1920

aagggaauac aucagauauu uggagcagcg uucaaaaguc ucuucggugg aaugucaugg     1980

uucagucaaa uacugauagg uaccuuauua auguggcuag gauuaaauac aaaaaacggu     2040

uccauuaguc uuauguguuu agcauuaggc ggaguauuaa uauucuuauc aacagcagua     2100

ucggcagacg uaggauguag uguagauuuc ucaaaa                               2136


<210>  2596
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long CDS opt

<400>  2596
agaauaauaa augcccguaa agaaaagaaa agaagaggug cagauacauc aguggguauc       60

guagguuuau uauuaacaac agcgauggca gcggaaguaa caagaagagg cucagcauau      120

uauauguacc uagacagaaa cgaugcggga gaagcaauau cuuuuccuac aacacuugga      180

augaauaaau gcuauauaca aauuauggac uuaggacaca ugugugaugc uacaaugagc      240

uacgaauguc caauguuaga ugaaggagua gaaccagaug acgucgauug cuggugcaac      300

acuacgagca cauggguugu uuacgguaca ugccaccaua agaaaggaga agcacgaaga      360

ucaagaaggg cugucacguu accgucacau ucaacaagaa aauugcaaac aagaucacaa      420

acaugguuag agucaagaga auauacaaaa cauuuaauua gaguagaaaa uuggauuuuu      480

agaaauccag guuuugcacu ugcagcagcu gcuauagcau gguuauuagg aucaagcaca      540

ucacaaaaag ucauauauuu agugaugauu uuacuaauug cacccgcaua cagcauaaga      600

uguauaggag uaucaaauag agacuucgua gaaggaaugu cgggaggaac uuggguagau      660

guaguauuag aacauggggg auguguaaca guaauggcuc aggauaaacc aacuguugau      720

auugaauugg uaacaacaac agugaguaau auggcugaag uaagaaguua uuguuacgaa      780

gcaaguauaa gugauauggc aagugauuca agguguccua cucaaggaga ggcauacuua      840

gacaaacaau cugacacuca auauguaugu aaacgaacau uagucgaucg agguuggggu      900

aauggaugug gucuauuugg aaaaggaucg cuaguaacau gugccaaauu ugcauguagu      960

aagaaaauga caggaaaaag uauucaacca gagaacuuag aauaucgaau uauguuauca     1020

guucauggaa gucaacauag uggaaugaua guaaaugaca ccggucauga aacggaugaa     1080

aauagagcaa aaguugagau aacaccaaau aguccuagag cagaagcaac acuaggaggu     1140

uucggaaguc uagguuuaga uugugaacca cgaacaggau uagacuuuag cgaucuauac     1200

uacuuaacua ugaacaauaa acacugguug guacacaaag aaugguuuca ugacauacca     1260

cuaccguggc acgcuggagc ugauacugga acaccacauu ggaauaauaa agaagcauug     1320

guagaguuua aagaugcaca cgcaaaaaga caaacaguag uaguauuagg aagccaagaa     1380

ggagcaguac auaccgcauu ggcuggugca uuagaggcag aaauggaugg ugcuaaaggu     1440

agguuaaguu caggccauuu aaaauguaga uuaaaaaugg auaaauuaag acuaaaaggu     1500

gucucauaca gcuugugcac agcagcauuc acauuuacaa aaaucccugc agaaacauug     1560

cauggaacgg uaaccguaga auuacaauau gcaggcacag auggaccuug uaaaguacca     1620

gcucaaaugg caguagauau gcaaacuuua acuccaguag gaagauuaau aacugcaaau     1680

ccagugauaa cggaaaguac ugaaaacagu aaaaugaugu uagaacuuga uccgccauuu     1740

ggagacagcu acauagugau agguguggga gaaaagaaaa uaacccauca uuggcaccga     1800

ucaggaucaa caauaggaaa ggcuuucgaa gcaacaguua gaggcgcaaa acgcauggcu     1860

guauugggag auacugcaug ggauuuugga aguguaggug gggcauuaaa caguuuagga     1920

aaaggaauac aucaaauuuu cggcgcagca uuuaaaucau uauucggagg aaugagcugg     1980

uuuucacaaa uacuuauagg aacacuacua augugguuag guuuaaauac uaaaaacgga     2040

aguauuucau uaauguguuu agcacucgga gguguauuaa uauuccuauc aacagcagua     2100

ucagcagacg uaggaugcag cguagacuuu aguaaa                               2136


<210>  2597
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long CDS opt

<400>  2597
agaauaauaa augcaagaaa agaaaagaaa agaagaggag cagaaacaag uguaggaauc       60

guuggauuau uauuaacaac agcaauggca gcagaaguaa cgagacgggg aucagcuuau      120

uacauguauu uggauagaaa ugaugcagga gaagcuauua gcuuuccaac aacacugggu      180

augaauaaau guuauauaca aauaauggac uugggucaua ugugcgacgc aacaauguca      240

uaugaauguc ccauguuaga ugaaggagua gaaccagacg auguagauug uugguguaau      300

acaacaagua cauggguagu auaugguacg ugccaucaua aaaagggaga agcaaggaga      360

ucaagaaggg caguaacauu accuagccau agcacucgaa aauuacaaac aagaagucag      420

acgugguugg aaucaagaga guauacaaaa cacuuaauac ggauagaaaa uuggauauuc      480

agaaacccag guuucgcucu ggcugccgca gcaauugccu ggcuauuagg gucaagcacc      540

ucacagaaag ugauuuacuu aguaaugaua cuauuaauag cuccugcaua cagcauuaga      600

uguauaggcg uuucaaaccg agacuuuguc gaaggaaugu cgggagguac augggucgau      660

guaguauuag agcacggagg augugugaca guaauggcac aagacaaacc aacagucgac      720

auagagcuag uaacaacaac uguaaguaau auggcagaag uuagaucaua uuguuaugaa      780

gccaguauaa gugauauggc uagugauucc agauguccaa cacaaggaga agcauaucuu      840

gauaaacaaa gcgauacaca auauguuugu aaaagaacuu uaguagauag gggauggggu      900

aacgggugug gacuauuugg aaaaggaucu uuaguuacau gugcaaaauu ugcauguagu      960

aagaaaauga caggaaaauc aauacaacca gaaaauuuag aauauagaau uauguuguca     1020

guacaugguu cacaacauag ugguaugaua guaaaugaca ccgggcauga aacagacgaa     1080

aauagagcaa aaguagaaau aaccccaaau agcccgagag cagaggcaac auugggugga     1140

uuuggaagcc uuggacuaga uugugaacca agaacagggc uagauuucag ugaccuguac     1200

uauuuaacaa ugaacaacaa acauugguua guccauaaag agugguucca ugauaucccc     1260

uuaccauggc acgcaggagc agacacggga acaccacacu ggaacaauaa agaagcauua     1320

gucgaauuua aagaugcaca ugcaaaaaga caaaccguag ugguauuagg aucacaagaa     1380

ggugcaguuc auacagcuuu agcuggagca cuugaagcag aaauggaugg cgcaaaagga     1440

agauuaucaa guggacauuu aaaauguaga uuaaaaaugg auaaauugag acuaaaaggu     1500

guaucauaca guuuauguac agcagcauuu acuuuuacaa aaauaccugc agaaacauua     1560

cacgguacag uaacaguaga aguacaauau gcaggaacag acggaccaug caaagucccg     1620

gcacaaaugg cuguagauau gcaaacacua acaccagugg gaagauuaau uacagcaaau     1680

ccaguaauaa cagaaucaac ugaaaauucg aaaaugaugc uagaauugga uccaccauuu     1740

ggagacaguu auauaguaau ugguguuggc gaaaagaaaa uaacacauca uuggcauaga     1800

ucaggaucaa cuauaggaaa agcauuugag gcuacagugc gaggagcuaa acgaauggca     1860

guguuaggug auacggcaug ggauuuugga aguguaggcg gugcccuaaa uucacuugga     1920

aaaggcauac accaaaucuu uggagcagca uucaaaucgc uauuuggugg aaugaguugg     1980

uuuagucaaa uauuaaucgg gacuuuauua augugguuag gauuaaauac uaaaaaugga     2040

agcauaucac uaauguguuu agcguuagga ggaguauuaa uuuucuuaag cacagccgua     2100

ucagcagaug ugggaugcag uguagauuuc ucgaaa                               2136


<210>  2598
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long CDS opt

<400>  2598
agaaucauaa augcuagaaa agaaaaaaaa agacggggag cagauacauc aguaggaaua       60

gugggauuau uacuaacaac ugcaauggcc gcagaaguua cacgaagagg gagugcauau      120

uauauguauc uagaccgaaa ugaugcagga gaagcaauaa gcuuuccaac aacccuugga      180

augaauaaau guuauauaca aauaauggau uuaggacaua ugugugacgc aacaauguca      240

uaugaaugcc cgauguuaga cgaaggggua gaaccagacg auguagacug cugguguaau      300

acaacaucaa cauggguugu auacggaacc ugccaucaua aaaaaggaga agcaagaaga      360

aguagaaggg cgguaaccuu accuucucau ucaacuagaa aacuucaaac uagaucacaa      420

acuugguuag aaaguaggga auauacaaaa cauuuaauaa gaguagagaa uuggauuuuu      480

agaaauccag gauuugcauu agcagcagca gcaauagcau gguuacucgg uucaucaaca      540

agucaaaaag uaauuuacuu aguaaugauu uuacuaauug cacccgccua cucaauuaga      600

uguauaggag uaucaaauag agauuucgua gaaggaauga guggaggcac uuggguugau      660

guaguauuag aacauggugg cuguguaaca gucauggcac aagauaagcc aacaguagau      720

auagaacuag uaacaaccac cguaucaaac auggccgaag uacgaucgua cuguuaugaa      780

gcaucaauua gugacauggc aucagauagu agauguccca cacaaggaga agcauaucua      840

gauaaacaaa gugauacuca auauguaugu aaaagaacau uaguagaucg agguuggggu      900

aauggaugug gacuauuugg aaaaggaucu cuagucaccu gugcaaaauu ugcuuguuca      960

aaaaaaauga caggaaaaag cauucaacca gaaaauuuag aauauagaau uauguuauca     1020

guacauggaa gccaacauuc uggaaugaua guaaaugaua cuggacacga aacagaugag     1080

aauagggcaa aaguugaaau aacaccaaau ucaccuagag cagaagcaac auuaggcggu     1140

uuugguagcu uggguuugga uugugaaccu agaacaggac uagauuuuag ugauuuauau     1200

uaucuaacaa ugaauaauaa acauuggcug guacauaaag aaugguuuca ugauauacca     1260

uuaccuuggc augcaggagc cgauaccggu acgccucauu ggaacaauaa agaagcacuu     1320

guagaauuca aagaugcaca cgcaaaacgu caaacaguag uaguauuagg aucacaagaa     1380

ggggcaguac auacggcguu agcaggcgca uuagaagcag aaauggaugg agcuaaaggu     1440

cgucuuucaa guggacauuu aaaauguagg uuaaaaaugg auaaacuacg auuaaaaggc     1500

guaucuuauu cauuauguac agcagcauuu acuuuuacca aaauaccagc agagacccua     1560

cauggaacag ucacgguaga aguacaauau gcugguacug auggaccaug uaaaguaccu     1620

gcucaaaugg ccguagauau gcaaacauua acaccagugg gaagacuaau aacagcaaau     1680

ccgguuauua cagaaagcau agaaaauuca aaaaugaugu uagaacuaga uccacccuuc     1740

ggugauucau auauaguuau aggcguaggg gagaaaaaaa uaacgcauca cuggcaucga     1800

ucaggaagua caauaggcaa agcauuugaa gcuacuguac guggagcaaa acgaauggcc     1860

guucucgggg acacugcuug ggacuucggc ucaguaggag gggcgcuaaa uagucucgga     1920

aaaggcauac accaaauuuu uggagcagcu uucaaaucau uauucggagg aaugucgugg     1980

uucagucaaa uauuaaucgg aacacuacua auguggcuag gacucaauac caaaaauggg     2040

aguauaaguu uaaugugucu agcauuagga gguguuuuaa uauuuuuauc aacugcuguu     2100

ucagcagacg uaggaugcuc aguugauuuu ucaaaa                               2136


<210>  2599
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long CDS opt

<400>  2599
agaauaauaa acgcaaggaa agaaaagaaa agaagaggag cugauacauc uguaggaauu       60

guaggguuac uauuaacaac agcaauggcu gcggagguaa caaggagggg cucagcauac      120

uauauguauc uagaccgaaa ugaugcagga gaagcaauaa guuucccaac aacauuggga      180

augaauaaau guuacauaca aauaauggau uuaggccaua ugugcgaugc aacaaugagu      240

uaugaaugcc cgaugcuaga ugaaggaguu gaacccgaug augucgauug uugguguaau      300

acaacaagua cuuggguagu auacggaaca ugucaucaua agaaagguga agcuagaaga      360

ucaaggagag cgguaacacu accgucacau ucaaccagaa aauuacaaac aagaucacaa      420

accugguuag aaaguagaga auauacaaaa cauuuaauaa gaguagaaaa cuggauauuu      480

agaaauccag guuuugcauu ggcugcugca gcuauagcau gguuacuagg aagcucaaca      540

agucagaaag uaauauaucu agucaugauu uuacuaaucg caccagccua caguauaagg      600

uguauaggag uaucaaauag agacuuugua gaagguauga guggagguac auggguagac      660

guaguacuag aacacggagg uuguguaacc guaauggccc aagauaaacc gacaguagac      720

auagagcuag uaacaacaac aguuucaaau auggcugaag uuagaagcua uugcuaugaa      780

gccucaauaa gcgacauggc uagugauuca agauguccca cacagggaga agcauauuua      840

gauaaacagu cagacacuca auauguaugu aagagaacau uaguagacag ggguugggga      900

aauggaugug gauuauucgg aaaaggaagu cuuguaacau gcgcaaaauu ugcauguucg      960

aagaaaauga caggaaaaag uauacaacca gaaaacuuag aauauagaau aaugcuuagu     1020

guacauggaa gucaacauag uggaaugaua guuaaugaua cuggucauga aacagaugaa     1080

aauagagcaa aaguagaaau aacuccaaac agcccuagag cagaagcuac auuaggagga     1140

uuugguaguu uaggacuaga uugcgagccu agaaccgggc uggacuuuuc ugauuuauau     1200

uacuuaacaa ugaacaauaa acauuggcua guacauaaag aaugguucca ugauauaccu     1260

uugccauggc acgcaggggc agauacagga acaccucauu ggaauaacaa agaagcucua     1320

guagaauuua aagaugcaca ugcaaaaaga caaacaguug uuguauuagg aagucaagaa     1380

ggagcaguac acacagcacu agcaggagca uuagaagcag aaauggaugg aguaaaaggc     1440

cggcuuagca gcggucaucu aaaauguaga uuaaaaaugg acaaacuaag guuaaaaggu     1500

guaaguuaua gucuguguac ugcggcauuu acauuuacaa aaauaccagc agaaacauua     1560

caugguaccg uaacaguaga aguacaauac gcaggaacgg auggacccug uaaaguuccu     1620

gcacaaaugg caguagacau gcaaacacua acaccugugg guaggcuaau aacugcaaau     1680

ccgguaauaa cagaaucaac ugaaaauagu aaaaugaugc uugaauugga ucccccuuuu     1740

ggagacaguu auauaguaau agggguagga gaaaagaaaa uaacucacca cuggcauaga     1800

agcggaucaa cuauagggaa agcguucgaa gcaacaguac gaggcgcaaa aagaauggca     1860

guauuaggag acaccgcaug ggauuuuggu aguguaggag gugcacuuaa uagccuaggc     1920

aaaggcauac accaaauauu cggagcugca uuuaaaucau uauuuggagg gaugagcugg     1980

uucucacaaa uauuaaucgg aacauuauua auguggcuug gacuaaauac caaaaaugga     2040

ucaauaucau uuaugugucu agcacuuggc ggaguauuaa uuuuucuaag uacagcaguu     2100

ucagcagacg uaggcuguag uguagauuuc agcaaa                               2136


<210>  2600
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long CDS opt

<400>  2600
cguauaauaa augcacgaaa agaaaaaaaa agaagaggag cugacacauc aguuggaauu       60

guaggauuau uauuaacuac agcaauggca gcagaaguaa caagacgagg aucugcuuau      120

uauauguauu uagauagaaa cgaugcggga gaagcaauau cauuucccac aacauuggga      180

augaauaaau guuacauaca aauuauggau uuaggacaua ugugugaugc aacaaugagc      240

uaugaauguc caaugcuaga cgaaggaguu gaaccugaug auguagacug uuggugcaau      300

acuacaucaa caugggucgu auauggaaca ugccaucaca aaaaagguga agcuagaaga      360

aguagaagag caguaaccuu accaucccac aguacacgaa aacuacaaac aaggagucaa      420

acaugguuag aaucaagaga auauaccaaa caucugauac gaguagaaaa uuggauauuc      480

agaaacccag gauucgccuu ggcagcugcc gcgauagcau ggcuacuagg aaguucaacu      540

ucacaaaaag uaauauacuu agucaugaua cuacuuauug cgccagcaua uagcauacga      600

uguauaggag ucagcaauag agauuuuguc gaaggaaugu cuggaggaac auggguagac      660

guaguauuag aacauggagg auguguaaca guaauggcac aagauaaacc aacuguagau      720

auugaauuag uuaccacaac aguaucaaau auggcagaag uaagaucaua cuguuaugaa      780

gcaaguauaa gcgauauggc aagugacuca agauguccaa cccaaggaga agcauacuua      840

gauaaacaaa gcgauacaca auauguaugu aaacgaacgu uaguagacag aggaugggga      900

aauggaugug gucuauucgg aaaagguagu uuagugacau gugcaaaauu ugcuuguagu      960

aaaaaaauga caggaaaauc cauucaacca gaaaaucuag aauauagaau aaugcugagu     1020

guacauggau cacaacacuc ugguaugaua gucaacgaca cuggacauga aacugaugaa     1080

aauagggcua aaguagaaau aacaccuaau ucaccaagag ccgaagcuac uuuagguggu     1140

uuuggcucau uaggcuuaga uugugaacca cgaacaggau uagauuuuuc agaccuauac     1200

uaccuaacaa ugaacaacaa acauugguua guucauaaag aaugguuuca cgauauacca     1260

cuaccuuggc augcaggugc agauacugga acuccacauu ggaauaacaa agaagcacua     1320

gucgaauuua aagacgcaca ugcaaaacga caaacaguag uaguuuuggg cucucaagaa     1380

ggugcaguuc auacagcacu agcuggugcu cuagaagcag aaauggaugg agcuaagggu     1440

aaacuaagua gcggacauuu aaaaugcaga cuaaaaaugg auaaauuacg acuaaaagga     1500

guaaguuauu cauuaugcac agcggcauuc accuuuacaa aaauaccagc agaaacauua     1560

cacggaacag uuacaguuga aguccaauau gcaggaacag auggaccaug uaagguaccu     1620

gcccaaaugg cuguagauau gcaaacacuu acaccuguug gaagacuuau uacagcuaac     1680

cccguaauaa ccgagucaac agaaaacagu aaaaugaugc uagaacuaga ccccccauuu     1740

ggugauaguu auauagugau agggguaggu gaaaaaaaaa uaacacauca uuggcauagg     1800

agcggaucaa caauagguaa agcauuugaa gccacaguaa gaggugccaa aagaauggca     1860

guguuaggag acacagcaug ggacuucgga ucagugggcg gugcacuaaa caguuuaggu     1920

aagggaauac aucagauauu uggugcugca uuuaaaucuu uauuuggagg aaugaguugg     1980

uuuagucaaa uuuuaauugg aacacuauua auguggcuag ggcuaaacac gaaaaacggc     2040

ucaauaucau uaaugugcuu agcauuaggu ggaguuuuaa uauuucuauc aacagcaguu     2100

agugcugacg uuggaugcuc cguagacuuc ucaaaa                               2136


<210>  2601
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long CDS opt

<400>  2601
agaauaauaa acgcuagaaa agaaaagaaa agaagaggag cugauacaag ugucgguauc       60

guuggacucu uauuaacaac ugcaauggcc gcagaaguga cacgaagggg uagugcauau      120

uacauguauu uagauagaaa ugaugcagga gaagcgauau cauuccccac aacacuagga      180

augaauaaau gcuauauaca aauuauggau uuaggucaca ugugugacgc aacaauguca      240

uaugaauguc caauguuaga cgaaggagua gaaccagaug auguagauug cugguguaau      300

acaacaucaa caugggucgu auacggaacu ugucaccaca agaaaggaga agcuaggaga      360

aguagacgag caguaacucu accuagucau ucuaccagaa aacuccaaac cagaucacaa      420

acaugguugg aaucaagaga auauacuaaa caccugauaa ggguagaaaa uuggauauuu      480

cguaacccag guuucgcauu agcagcagca gcaaucgcau gguuauuagg aagcagcaca      540

ucacaaaaag uaauauauuu agugaugaua uuacuaauag cgccugcaua uaguauuaga      600

uguauaggag uaucaaaucg agacuuuguu gaaggaaugu caggaggaac auggguagac      660

guaguucuug aacacggagg auguguaaca guaauggcac aagacaaacc aacgguagau      720

auagaauuag uaacaacgac aguaucaaac auggcagaag uaagaucuua cuguuacgaa      780

gcguccauau cagauauggc aagcgauuca agaugcccaa cccaagguga agcauaucua      840

gauaaacaaa gugauacaca auauguaugu aaaaggacuc uagucgacag aggguggggu      900

aauggaugug gacuauuugg aaaaggauca uuaguaacau gugcaaaauu ugcauguuca      960

aagaaaauga ccggaaaaag cauccaacca gaaaaucuug aauaccgaau uaugcuauca     1020

guacauggau cacaacacuc aggaaugaua guaaaugaca caggucauga aacagaugaa     1080

aacagggcaa aaguagaaau aacaccaaau ucaccuagag cagaagcaac ccucggaggu     1140

uuuggaucau uaggauuaga uugugaaccu agaacaggac uagauuuuuc agacuuauac     1200

uacuuaacca ugaacaauaa acauugguua guacacaaag aaugguuuca ugacauaccu     1260

uugccauggc acgcaggagc cgauaccgga acaccacacu ggaacaauaa agaggcuuua     1320

guagaauuua aagaagcaca ugcaaaacga caaacaguag uaguauuagg aagucaagaa     1380

ggggccguac acacagcacu agcaggagca uuagaagcag aaauggaugg agcuaaagga     1440

agacuaucaa guggacauuu aaaaugcaga cuaaaaaugg auaaacuaag acuaaaaggg     1500

gucagcuaca gucuauguac agccgcauuu acauuuacca aaauaccagc agaaacacua     1560

cacggaacag uaacaguaga aguacaauac gcaggaacug acggaccaug uaaaguaccc     1620

gcacaaaugg caguagauau gcagacauua acuccagucg gcagauuaau aacugcaaac     1680

ccugucauaa cagaaaguac agaaaauuca aagaugaugu uagaacuaga uccacccuuc     1740

ggcgacagcu auaucguuau aggaguggga gaaaagaaaa uaacacauca uuggcaucga     1800

ucagguagua caauaggaaa ggcuuuugaa gcaacaguaa gaggugccaa aagaauggcu     1860

guacuuggag auacagcaug ggauuuugga ucaguaggug gugcuuuaaa uaguuuagga     1920

aaaggaauac aucaaauuuu uggagcagca uuuaaaucau uauuuggagg aaugucaugg     1980

uucucacaaa uauuaauagg aacucuauua auguggcucg gauuaaauac caaaaauggg     2040

ucaauaaguu uaaugugccu agcauuaggg gguguauuaa uauuuuuauc aacagcagua     2100

agugcagaug uuggauguuc agucgauuuu aguaaa                               2136


<210>  2602
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long CDS opt

<400>  2602
cgaauaauaa augcuagaaa agaaaagaaa agaagaggag cagauacuuc aguuggaaua       60

guagguuuac uucucaccac ugcaauggca gcagaaguaa cacguagagg cucagcauac      120

uacauguauu uagaucgaaa ugaugcagga gaagcaauau cauucccaac aacacucgga      180

augaauaaau guuauauaca aaucauggau uugggacaua ugugugaugc cacuaugagc      240

uacgaauguc cuaugcuaga cgaaggagua gaaccugaug auguagauug uugguguaac      300

acuacaagca cauggguagu uuauggcaca ugucaucaua agaaagguga agcaagaaga      360

ucaagacgag cgguuacacu gccuucucac ucuacaagaa aacuacaaac aagaucacaa      420

acuugguuag aaaguagaga auacacgaaa cauuuaauaa gaguagaaaa cuggauuuuc      480

agaaaucccg gauuugcuuu agcugcagca gcuauagcuu gguuauuagg aagcaguaca      540

agccaaaaag uaauauauuu aguaaugauu cuacuuauag cuccagcaua uucaauaagg      600

uguauaggag uuucaaauag agauuuugua gaaggaaugu cagguggaac auggguagau      660

gucguucuag aacauggcgg augcguaaca gucauggcac aagauaaacc aacaguagau      720

auugaauuag uaacaacaac uguaaguaau auggcagaag uaagaucgua uugcuacgaa      780

gcaucaauaa gugauauggc aucagacuca agaugcccaa cacaaggaga agcauaucua      840

gauaaacaau cagacacgca auauguuugu aagcgaaccu uaguagauag agguuggggu      900

aacgggugcg gauuauuugg aaaaggcagu cuaguaacau gcgcuaaauu ugcguguagu      960

aaaaagauga cuggaaaauc aauucaacca gaaaaucuag aauauagaau aaugcuguca     1020

guacaugguu cacaacauag uggaaugaua guaaaugaca cgggucacga aacagaugaa     1080

aauagagcga aaguagaaau aacaccaaau agcccacgag cugaagcuac auugggaggu     1140

uucgguucuu uagguuuaga cugcgaaccg agaacaggau uagacuucag cgaucuauau     1200

uauuuaacaa ugaauaauaa acacugguua guacauaaag aaugguuuca ugacauucca     1260

uuaccauggc augcaggagc agauacaggu acaccacauu ggaacaauaa agaagcauua     1320

guagaauuua aagaugcaca ugcaaaaaga caaacaguag uaguuuuagg uagucaagaa     1380

ggagcaguuc acacagcacu agcuggugca uuagaagcug aaauggaugg ugcgaaagga     1440

agacugaguu ccggacaccu aaaaugcaga cuaaaaaugg acaaacuaag acuaaaaggu     1500

guaucauaca gcuuauguac agcagcauuc acauucacaa aaauucccgc agaaacauua     1560

cauggaacag uaacaguuga aguccaguac gcgggugcag augguccuug caaaguaccc     1620

gcccaaaugg cagucgacau gcaaacuuua acaccuguag gaagauugau aacagcaaau     1680

ccaguaauaa cagaaucaac agaaaacagu aaaaugaugu uggaacuuga uccaccauuc     1740

ggugacaguu acauaguaau agggguugga gaaaagaaaa uaacacauca uuggcauaga     1800

aguggaagca caauaggaaa agcauuugaa gcaacaguaa gaggcgcaaa acgcauggca     1860

guacuuggag auacagcuug ggacuuuggg agcguuggag gagcccuaaa cagucuugga     1920

aaaggaauac aucagauauu cggagcugca uucaaaaguc uauuuggagg aaugucaugg     1980

uuuagucaaa uauuaaucgg uacauuauua auguggcuag gcuuaaauac gaaaaaugga     2040

aguauaaguc uuaugugucu agcauuaggu gguguacuaa uauuuuuaag uacugcagug     2100

agugcagacg uagguuguuc aguugacuuc aguaaa                               2136


<210>  2603
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long CDS opt

<400>  2603
agaauuauaa augcaggcaa agaaaaaaaa agacgaggug cagauacuaa cgugggaaua       60

guaggauuau uacuaacaac agcgauggca gccgaaguaa cuagacgagg aagcgcauau      120

uauauguauc uagaucgaaa ugaugcggga gaagcaauaa guuucccaac aacauuagga      180

augaacaaau gcuauauaca aaucauggau cuuggacaca ugugugaugc aacaaugagc      240

uaugaauguc caauguuaga ugaaggaguu gaaccugaug acguggauug uuggugcaac      300

acgacaucaa cauggguagu auauggaaca ugccaucaca aaaaaggcga agccagaaga      360

agcagaagag caguaacguu accuagucau agcacaagga aauuacaaac aagaagucaa      420

acuuggcuug aaucaagaga auacacaaag caucucauca gaguugaaaa cuggaucuuu      480

cgaaauccag gauuugcuuu agcagccgca gccauagcau gguuacuagg aucaucaaca      540

agucaaaaag uaauauacuu ggucaugaua cuacuaauug caccagcuua uaguauacgu      600

ugcauaggag uaaguaacag ggauuuugua gaaggaaugu cuggaggaac auggguugau      660

guaguccuag aacacggagg auguguuacg guaauggcac aagauaaacc aacaguagau      720

auagaauuag uaaccaccac aguuucaaau auggcagaag uacgaucgua uuguuacgaa      780

gcuaguauaa gugacauggc aucagauuca agaugcccaa cacaaggaga agccuauuua      840

gauaaacaaa gugacacaca auacguaugu aaaagaacac uaguagauag gggaugggga      900

aaugguugcg guuuauuugg gaaggguagu uuaguaacgu gugcaaaauu ugcauguagu      960

aaaaagauga cuggcaaaag cauccaaccu gaaaauuuag aauauagaau aauguuauca     1020

guacauggaa gucaacacuc aggaaugauc guaaacgaua cuggacacga aacugaugaa     1080

aaucgagcaa aaguagaaau uacaccaaac ucuccacgag cagaagcaac auuaggagga     1140

uuuggaucau uaggccuaga uugcgaaccc cgaacaggac uagauuucag cgauuuauau     1200

uauuuaacaa ugaauaauaa acauugguua guacauaaag agugguuuca ugacauaccu     1260

cuaccguggc augcaggagc agauacuggu acaccacauu ggaauaauaa agaagcacug     1320

gucgaauuca aagaugcaca ugcaaaacga caaacaguag uaguauuagg cagucaagaa     1380

ggagcgguac auacagcacu agcaggagcc cuagaagcag aaauggacgg ugcaaaagga     1440

agauuaagua guggacaucu gaaauguaga uuaaaaaugg auaaacuaag auuaaaagga     1500

gucucauauu cauuauguac agcagcauuu acauuuacaa aaauacccgc cgaaacauua     1560

cauggaacag uaacagugga aguacaauau gcuggaacag auggaccuug uaaaguucca     1620

gcucaaaugg caguagacau gcaaacauua acacccguag guagacuaau uacagcaaac     1680

ccuguaauaa cagaaagcac agaaaacuca aaaaugaugc uagaauuaga ucccccauuu     1740

ggugauaguu auauuguuau aggaguugga gaaaaaaaaa uaacacauca uuggcacaga     1800

uccgguucga caauuggaaa agcauuugaa gcuacuguaa gaggugcaag gcggauggcg     1860

guauugggag acacugcuug ggacuucgga ucaguuggag gagcacuaaa cucacuagga     1920

aaagguauac aucaaauuuu uggugcagcc uuuaaaucau uauucggagg cauguccugg     1980

uucucacaaa uacuaauagg uacacuucua augugguuag gacuaaauac gaaaaaugga     2040

ucaauaagcu uaauguguuu agcccuaggg ggaguccuaa uauucuuauc aacugcagua     2100

ucagcagaug uagguuguag uguagauuuu ucuaaa                               2136


<210>  2604
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long CDS opt

<400>  2604
agaauaauaa augcaagaaa agaaaaaaaa cgccgaggag cagaaacuag uguagggaua       60

guagguuuac uuuuaacaac agcaauggca gcagaaguua caagaagagg aagugcuuau      120

uauauguauu uagaccgcaa agaugcagga gaagcaauaa guuuucccac aacguugggu      180

augaauaaau guuacauaca aauuauggac uuaggacaca ugugcgaugc aacuaugagc      240

uacgaaugcc cuaugcuaga ugaagggguc gaaccagaug acguugauug uugguguaac      300

acaacaucaa cauggguagu cuaugguaca ugccaucaua aaaaagguga agcucgacga      360

ucaagaagag caguaacacu accuagucau aguacuagaa aacuacaaac aagaagucaa      420

acauggcuag aaaguagaga guauacaaaa caccuaauaa gagucgaaaa uuggauuuuu      480

agaaacccag gauuugcauu ggcggcagcu gcuauugcau gguuacuagg aaguucaacu      540

ucacaaaaag uuauauaucu aguaaugaua cuauuaauag caccugcgua uucaauuaga      600

uguauaggag uuucgaauag agauuuugua gaagggaugu cagguggaac auggguagac      660

guaguacuag aacacggagg auguguaaca gucauggcac aagauaaacc aacaguugac      720

auagaauuag uaacaacaac aguuaguaau auggcagaag ucagaucaua uugcuaugaa      780

gccagcauau cugauauggc aagcgacuca agauguccaa cacaagguga agcguaucuu      840

gauaaacaau cagauaccca auauguaugu aagcgaacau uaguagauag agggugggga      900

aauggaugcg gacuguuugg aaaaggaagu cuagugacau gcgcaaaauu cgcauguuca      960

aaaaaaauga caggaaaaag cauacaaccg gaaaauuuag aauauagaau aauguuaagu     1020

guacaugguu cacaacauag uggaaugaua gucaaugaua cuggacacga aacagaugaa     1080

aaucgagcaa aaguugaaau aaccccuaau ucaccaagag cagaagcuac auuaggaggu     1140

uuuggaaguc uaggauuaga cugugaacca agaacagggc uagacuuuag cgauuuauau     1200

uauuuaacaa ugaauaauaa acauugguug guacauaaag aaugguuuca cgacauccca     1260

cuaccguggc augcuggagc cgacacuggu acaccacauu ggaauaauaa ggaagcauua     1320

guagaauuua aagaugcaca cgcaaaaagg caaacaguug uagucuuggg cagucaggag     1380

ggagcaguuc auaccgcucu agcuggagca uuagaagcag aaauggaugg agcaaaaggu     1440

agauuaucau cuggacaccu aaaauguaga cuuaaaaugg acaaauugag acuuaaaggu     1500

gucucauauu ccuuauguac agcugccuuu acauuuacaa aaaucccagc ugaaacauua     1560

cauggaacag ugacagugga aguacaauac gcaggaacag auggaccuug uaaaguucca     1620

gcacaaaugg caguugauau gcaaacacua acaccaguug gcagauuaau uacggccaac     1680

ccagugauaa cagaaaguac agaaaauuca aagaugaugu uagaacuaga uccuccuuuu     1740

ggagauucau auauugucau aggaguagga gaaaaaaaaa uaacucauca uuggcauaga     1800

ucaggaucaa caauaggaaa agcauucgaa gcaacaguua gaggagcuaa aaggauggcc     1860

guacuaggag acacagcaug ggauuuugga ucagugggag gagcauuaaa cucauuggga     1920

aaagggauac accaaauuuu uggcgcagca uuuaaaucuu uauuuggggg caugucuugg     1980

uucagucaaa uauuaauugg aacacugcuc auguggcuug gacuuaauac aaaaaacgga     2040

ucaauaagcu uaaugugucu ugcauuagga ggaguucuaa uauuuuuaag uacugcagua     2100

ucugcagaug uaggauguag uguggauuuc aguaaa                               2136


<210>  2605
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long CDS opt

<400>  2605
agaauaauaa acgcaagaaa ggaaaaaaaa agaagaggag cagauacaag uguaggaaua       60

guaggcuuac uacuuaccac agcaauggcu gccgaaguaa caagaagagg aucagcauau      120

uacauguauu uagauagaaa cgaugcagga gaagcaaucu cuuuuccaac cacacucggg      180

augaacaaau guuacauaca aauaauggac uuaggacaua ugugugaugc aacuaugagu      240

uaugaauguc cuaugcuaga ugaggggguc gaaccugaug auguugauug uugguguaau      300

accacuagca cauggguagu guaugguaca ugucaucaca aaaaagguga agcaagaaga      360

ucaagaagag caguaacauu accaagucau agcaccagaa aauuacaaac aagaucgcaa      420

acaugguuag aaaguagaga auauacuaaa caucuaauaa ggguagaaaa cuggauauuu      480

agaaauccag gcuuugcauu agcagcggcu gcaaucgcau gguuacuagg uaguucaaca      540

ucacaaaaag uuauauaucu aguuaugaua cuauuaauag caccagcaua cucaauaaga      600

ugcauaggag uaaguaauag agauuucgua gaagguauga gugggggcac auggguugac      660

guuguuuuag aacauggugg auguguuacu guaauggcac aagacaaacc aacuguagau      720

auagaacuag uaacgacaac aguaucaaac auggcugaag ugagaucaua uuguuaugaa      780

gccaguauaa gugauauggu cagugauuca agauguccaa cacaagguga agcauauuua      840

gauaaacaau cagacacuca auacguuugu aaaaggacuc ucguagacag aggaugggga      900

aacggaugug gauuauuugg uaaagggucu uuaguaacau gcgcaaaauu ugcguguagc      960

aagaaaauga ccggaaaaag uauucaaccu gaaaauuuag aguauagaau uaugcuaagu     1020

guacacggaa gucaacauuc agguaugaua guuaacgaua caggucauga aacagaugaa     1080

aauagagcua aaguagaaau aacaccaaau ucacccaggg cagaagcaac auuagggggc     1140

uucggaucau uaggacuaga uugugaaccu cgaacugggc ucgauuuuag ugaucuuuau     1200

uauuugacaa ugaauaauaa acacuggcuu gugcauaaag aaugguuuca cgauauacca     1260

uuaccauggc acgccggagc ggauacagga acuccacauu ggaacaacaa agaagcauug     1320

guagaauuua aagacgccca ugcuaaaaga caaacagucg uaguacuagg aucucaagaa     1380

ggugcaguac auacagcgcu agcaggagcg uuagaagcgg aaauggaugg ugcuaaagga     1440

agauuaagca gcggacaucu gaaaugcaga uuaaaaaugg acaaauuaag auuaaaagga     1500

gucucauauu cauuauguac agcugcauuu accuucacaa aaauaccagc agagacuuug     1560

caugguacag uaacaguaga aguacaauau gcuggaacag acggucccug uaaaguaccu     1620

gcacaaaugg cgguagauau gcaaacacua acucccguag gaagacuaau aacagcuaau     1680

ccgguuauaa ccgaaaguac ugaaaauagc aaaaugaugu uagaauugga cccaccauuu     1740

ggagauaguu auauuguuau agguguaggc gaaaaaaaaa uaacucacca uuggcauaga     1800

agugguagua caauaggcaa agcauucgaa gcaacuguaa gaggagcaaa aagaauggcu     1860

guacuaggcg auacagcaug ggauuucggu agcguaggag gagcacuaaa cucuuuaggu     1920

aaagguauuc accaaauauu uggugcagca uuuaaguccc uauucggagg aaugagcugg     1980

uuuagucaaa ucuuaauagg aacucuacua augugguuag gacuaaauac aaaaaauggu     2040

aguauaaguu uaaugugcuu agcacuugga ggaguauuaa uuuuucuaag cacagcagua     2100

ucagcagacg uuggaugcuc uguagauuuc ucaaaa                               2136


<210>  2606
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long CDS opt

<400>  2606
agaauuauaa augcccgaaa agaaaagaaa agaagaggag cagauacaaa uguagguaua       60

gucggccuac uacuaacaac agcaauggca gcagaaguua cacgccgugg aucagcuuau      120

uauauguacc uagauagaaa ugaugcagga gaagcgauua guuuuccgac aacauuaggu      180

augaauaaau guuacauaca aauaauggac cugggacaca ugugcgacgc uacaaugagc      240

uaugaaugcc caaugcuaga cgaaggagua gaaccagaug acguagacug cugguguaau      300

augacaucaa caugggucgu auauggaaca ugccaucaua agaaaggaga agcaagaaga      360

ucucggagag cgguaacucu cccaagucau aguacuagaa aacuacaaac aagaucucaa      420

accuggcuag aaucaagaga auauacuaaa caucuaauaa gaguagaaaa uuggauauuu      480

agaaacccug guuucgcccu agcagcugcu gcaauagccu ggcuacuagg aaguaguaca      540

agccaaaaag uaauauauuu aguaaugaua uuacucauug cuccggcgua uaguauuaga      600

uguauuggag uaaguaauag agacuucgua gaaggaaugu cgggaggcac auggguagau      660

guaguacuag aacauggagg auguguaacg guuauggcuc aagauaaacc aacaguagau      720

auagaauuag ucacuacaac aguaucaaau auggcagaag uaagaucuua cuguuaugaa      780

gcaucaauaa gugauauggc uagcgacucu agaugcccaa cccaaggaga agcauacuua      840

gauaaacaau cagauacaca auauguaugu aaaagaacau uaguagacag agggugggga      900

aauggaugug gauuauucgg aaaaggaucc cuaguaacau gcgcaaaauu ugccuguuca      960

aagaaaauga caggaaaauc aauacaacca gaaaacuuag aauauagaau aauguuaagu     1020

guacacgguu cacaacauuc aggaaugaua guaaaugaua ccggucauga aacugaugaa     1080

aauagagcaa aaguggaaau caccccaaau ucaccaaggg cagaagccac ucuaggaggg     1140

uuugguucac uaggacuuga uugugaacca agaacaggac uagauuuuuc agauuuauau     1200

uauuuaacca ugaauaauaa acauugguua guucauaaag aaugguuuca ugauauuccc     1260

cuuccauggc augcaggagc agacacagga acaccucauu ggaacaacaa agaagcucua     1320

guagaauuua aagaugcaca cgcaaaaaga caaacuguag ucguucuagg aucacaagaa     1380

ggagcaguac acacagcacu agcaggagca cucgaagcag aaauggaugg agcaaaagga     1440

cgacuaagca guggacauuu aaaaugucga cuuaaaaugg auaaauuaag auuaaaagga     1500

guaaguuacu cauuauguac agcagcauuu acauucacaa aaauacccgc agaaacccua     1560

cauggaacag uaacugugga aguccaauac gcuggcacag auggaccaug uaaaguacca     1620

gcucaaaugg caguagacau gcaaacauua acuccaguag guagauuaau aacugcaaau     1680

ccugucauaa cagaaucaac ggaaaacucc aaaaugaugu uagaacuaga cccaccauuu     1740

ggagacaguu auauaguuau agguguaggu gaaaagaaaa uuacacauca uuggcauaga     1800

aguggaucaa ccauaggaaa agccuuugaa gcaacaguaa gaggagccaa aaggauggca     1860

guacuuggag acacagcaug ggacuuugga agcguaggag gagcauuaaa cagccuggga     1920

aaaggaauac aucaaauauu cggagcagca uuuaaaagcc uuuuuggagg uaugucgugg     1980

uucucccaaa uccuaauugg aacucucuua auguggcuag gacuuaauac caaaaacgga     2040

ucaauaucau uaaugugucu agcauuagga gguguacuaa uauuucuaag uacagcagua     2100

ucagcagacg uaggauguuc aguagacuuc ucaaaa                               2136


<210>  2607
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long CDS opt

<400>  2607
agaauaauaa acgcuaggaa agaaaaaaaa agaagagggg cagacacaag uguagggaua       60

gucggacuau uauuaacuac agcaauggca gcagaaguua caaggagagg uucugcauac      120

uauauguauu uagaucgaaa ugacgcaggc gaggcuauaa guuuucccac aacucucgga      180

augaauaaau guuauauuca aauaauggau cuuggacaca ugugugaugc aaccauguca      240

uaugaauguc caauguuaga ugaaggagua gagccagacg auguagacug uugguguaac      300

acuacaucaa cuuggguagu uuacggaaca ugucaucaua aaaaaggaga agcgagaagg      360

aguagaagag caguaacauu accuucacau ucaaccagga aauuacaaac aagaagccaa      420

accugguuag aaucaagaga auauacaaag cauuuaauac gcguggaaaa cuggauauuu      480

aggaacccag gcuucgcuuu agcagcggcu gcgauagcau ggcuccucgg aucaucaacg      540

ucacaaaaag uaauauauuu aguaaugauu uuauugauag caccagcaua uucaauacga      600

uguauuggcg ugucaaaucg agauuuuguu gaaggaaugu ccggaggaac uuggguagau      660

guaguauuag aacauggagg auguguaaca guaauggcac aagacaaacc uacaguugau      720

auagaauuag uaacaacaac cguaucaaau auggcagagg uaagaagcua uuguuaugaa      780

gcuucaauau cagauauggc uucagauagc agauguccaa cacagggaga agcuuaucua      840

gacaaacaau cagacacaca auauguaugu aaaaggacac uaguagauag agguugggga      900

aaugguugug gucuuuucgg uaaaggaagc uuaguaaccu gcgcaaaauu ugcuuguagu      960

aaaaaaauga cuggaaaaag cauucaacca gaaaauuuag aauauagaau aauguuaagu     1020

guacacggcu cucagcauag cggaaugaua guaaaugaua ccggccacga aacugacgaa     1080

aauagagcca aagucgaaau uacuccuaau ucuccaaggg cugaagcugc auuagguggu     1140

uuuggaucgc uaggccuuga cugcgaacca agaacaggau uagacuucag ugaucuauau     1200

uauuuaacca ugaauaauaa acacugguua guccauaaag aaugguucca ugauauaccg     1260

cuaccauggc acgcaggugc agauacaggu accccacacu ggaauaauaa ggaagcauua     1320

gucgaauuua aagaugcaca cgcgaaaaga caaacaguag uaguauuagg aucacaagaa     1380

ggggcuguac acacagcacu agcuggagca cuagaagcag aaauggaugg ugccaaaggc     1440

agauuaagua guggacaucu uaaauguaga cuuaaaaugg auaaauuacg acuuaaaggu     1500

guaaguuauu cacuauguac agcagcauuu acuuuuacua aaauuccagc agaaacacuu     1560

cauggcaccg uaacuguuga aguacaauac gcaggaacug augguccuug uaaaguucca     1620

gcacaaaugg caguggauau gcaaacauua accccaguag gcagauugau aacugcaaau     1680

ccgguaauua cagaaaguac agaaaacagu aaaaugaugc uugagcuuga uccaccauuu     1740

ggggacucuu acauaguaau aggagucggu gaaaaaaaaa uaacacacca cuggcacaga     1800

ucagguucaa cuauaggaaa agccuucgaa gcuacaguaa gaggugcaaa aagaauggca     1860

guauuaggag auacagcaug ggacuucgga ucuguaggag gagcauuaaa uucauuagga     1920

aaaggaauac aucaaaucuu cggagccgca uuuaaaaguc uauucggugg aaugucuugg     1980

uuuagccaaa cauuaauagg cacuuuauua auguggcuag guuuaaauac aaaaaauggu     2040

ucaauuaguu uaauguguuu agcauuagga gguguacuaa uauucuugag uaccgcagua     2100

ucagccgaug ucgguuguuc uguagauuuu agcaaa                               2136


<210>  2608
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long CDS opt

<400>  2608
aggauuauaa acgcacgaaa agaaaaaaaa agaagagggg cagaaacaag cguaggaauu       60

guaggucuac uauuaacaac agcuauggcu gcugaaguaa cacgaagagg aucagcauau      120

uacauguauu uagauagaaa ugaugcggga gaagcaaucu cauucccuac aacguuaggg      180

augaauaaau guuauaucca aauaauggau cuaggccaca ugugcgaugc uacaaugucc      240

uaugagugcc caaugcuaga ugaaggagua gaaccagaug auguagauug uugguguaau      300

acaacaagua cauggguagu auaugguaca ugucaccaca aaaaaggaga agcgaggcgg      360

ucaaggagag caguuacauu accaucacac aguacaagaa aacuacagac acgaucacaa      420

acaugguugg aaaguaggga auacacuaaa caucuaauac guguugaaaa uuggaucuuu      480

agaaacccag gguucgcucu agcagcggca gcuauagcau gguuacuagg uucuucaaca      540

ucgcaaaaag uaauauaccu cguaaugauu cuacuaauag ccuuagcuua cucaauaaga      600

ugcauaggcg uauccaauag agauuuugua gaaggaaugu caggaggaac uuggguagau      660

guaguuuuag aacacggagg cuguguaaca guaauggcac aggacaaacc uacaguagau      720

auagaauuag uaacaacaac aguaucuaau auggcagaag uucgaucaua uuguuaugaa      780

gcaucgauuu cagauauggc aagcgauagu agauguccua cgcaagguga agcauauuua      840

gauaaacaau cugauacuca auauguaugc aaaagaacau uaguagauag ggguugggga      900

aauggaugug gacuauucgg aaaaggaagc uuggucacuu gugcaaaauu ugcauguuca      960

aaaaaaauga caggaaaauc aauacaacca gaaaacuuag aauacagaau aaugcugagc     1020

guacauggaa gccaacauag cggaaugaua guaaacgaca caggacauga aacagaugaa     1080

aacagagcaa aaguagaaau aacucccaau ucaccaagag cagaagcaac ucucggagga     1140

uucggaagcu uaggacuaga cugugaaccg agaacagguc uagacuucuc ugaucucuac     1200

uaucuaacua ugaauaacaa acauuggcua guucauaaag aaugguucca ugacauacca     1260

uuaccauggc augcuggagc cgauacagga acaccacauu ggaacaauaa agaagcucua     1320

guugaauuua aagaugcaca ugcaaaaaga caaaccgucg uuguauuagg aucacaagag     1380

ggagcugucc auacagcauu agcaggagca uuagaagccg aaauggaugg agcaaaagga     1440

agacuaucau caggacauuu gaaauguaga uuaaaaaugg auaaauuaag acuaaaaggg     1500

guaucauacu cacuauguac cgcagcauuu acauuuacca aaauaccagc agaaacauua     1560

cauggaacag uuacagugga aguccaauau gccggaacug augguccaug caagguaccc     1620

gcccaaaugg caguagacau gcaaacacua acaccagugg gaagauuaau aaccgccaau     1680

ccaguaauua cagaaucaac agaaaauuca aaaaugaugc ucgaauuaga uccgccauuc     1740

ggagauagcu acauuguuau uggaguagga gaaaaaaaaa uuacacacca uuggcaccga     1800

agcggaagca cuauuggaaa agcauuugaa gcuacuguaa gaggagcaaa acgaauggca     1860

guacuuggag auacugcuug ggauuuugga aguguaggug gcgcacuaaa cagcuuaggu     1920

aaaggaauac aucaaauauu cggcgccgca uucaaaagcu uguuuggggg aaugucaugg     1980

uucucacaaa uauugauagg uacacuuuug auguggcuag gacuaaauac aaaaaacggu     2040

ucaauaaguc uuaugugucu agcccucggu ggaguacuua uauuuuuaag uaccgcagua     2100

agcgcagaug uuggcuguag uguagacuuu ucaaaa                               2136


<210>  2609
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long CDS opt

<400>  2609
agaauaauaa augcgagaaa agaaaaaaaa agaagaggag cugauacaag uguuggaauu       60

gugggucucu uauuaacaac agcaauggca gcagaaguaa caagaagagg gucagcauac      120

uauauguauc uagauaggaa ugaugccggc gaagcuauau cauuuccuac aacauuagga      180

augaauaaau gcuauauaca aauaauggau cuuggucaua ugugugaugc aaccaugagu      240

uaugaauguc cgauguugga cgaaggaguu gaaccagaug auguagacug cugguguaau      300

acaacaagua caugggucgu auauggcaca ugucaucaua aaaaaggaga agcgagaaga      360

ucgcguagag cuguaacacu accaucacau aguaccagaa aacuacaaac gagaagucaa      420

acauggcuag aaucaagaga auauacaaaa cacuuaauac gaguagaaaa uuggauauuu      480

agaaacccag gauucgcauu agcagcagcg gcaauugcau gguuauuagg aucaaguacg      540

agucaaaaag uaauauauuu aguaaugaua cuauuaauag caccugcaua cucaauaaga      600

uguauaggaa uaaguaaucg agauuucgug gaaggaaugu caggagguac auggguagau      660

guaguucuug aacauggagg auguguaacu guaauggccc aagacaagcc aacaguagau      720

aucgaauugg uaacaacgac aguuaguaau auggcagaag uacgaaguua uuguuacgaa      780

gcaucuauuu cugauauggc aucagacagu agauguccaa cacaaggaga ggcuuaucug      840

gacaaacaaa gugacacaca auauguaugu aaacguacgc uaguugaucg aggaugggga      900

aacggaugug gguuauucgg aaaagggagu uuaguaacau gugcaaaauu ugcauguucg      960

aaaaagauga cgggaaaaag cauacaacca gagaauuugg aauauagaau aaugcuaagu     1020

gugcacggau cacaacacuc agguaugaua gucaaugaua cuggacauga aaccgaugaa     1080

aaucgugcaa aaguagaaau uacaccaaau aguccaagag cagaagcaac acuaggcgga     1140

uucgggauau uaggauuaga uugugaacca agaacaggac uagauuuuag cgaucuauac     1200

uacuuaacca ugaauaacaa acauugguua guacauaaag agugguucca ugauaucccu     1260

cuuccauggc acgcuggugc agauacagga acaccacauu ggaacaacaa agaagcacua     1320

guagaauuua aagaugcaca cgcaaaaaga caaacaguag uaguauuagg aagccaagag     1380

ggagcaguac auacagcacu agcaggugca uuagaagcag aaauggacgg agcgaaaggu     1440

agauugagua guggacauuu aaaaugcaga uuaaaaaugg acaaacuaag acuuaaagga     1500

guaaguuaca gucuuuguac agcagcauuu acuuucacaa aaauaccagc ugaaacacuu     1560

cauggaacag uuacuguaga aguucaauau gcagguacag auggaccaug uaaaguaccc     1620

gcacaaaugg caguagauau gcaaacccug acaccaguag gcagacuaau aacagcgaac     1680

ccaguaauaa cagaaagcac cgaaaacuca aaaaugaugc uagaauuaga cccaccauuu     1740

ggagacaguu auauaguuau agguguugga gaaaaaaaaa uaacacauca cuggcacaga     1800

agugguagua caauaggcaa agcauuugaa gcuacuguca gaggcgcaaa aagaauggcc     1860

guauuaggag auacagcaug ggacuuuggg agcguuggag gugcauugaa uagccucggu     1920

aaagggaucc aucagauauu uggagcagca uuuaaaagcu uauucggagg aaugucaugg     1980

uucucacaaa uauuaauugg uacauuauua augugguuag guuugaacac aaagaauggu     2040

ucaauuucau uaaugugcuu agccuuagga ggaguauuaa uauuucuuag uacagcagua     2100

ucggcugaug uuggauguuc cguagauuuu aguaaa                               2136


<210>  2610
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long CDS opt

<400>  2610
agaauaauaa augcaagaaa agaaaagaaa agaagaggag cagauacaag uguaggaaua       60

gugggucuau uauuaacgac ugcaauggca gccgaaguga caagacgagg aagcgcauau      120

uauauguauc uagauaggaa ugaugcugga gaagcaauuu cauuuccaac cacuuuagga      180

augaauaaau gcuauauuca aauaauggau cuaggacaua caugugaugc aacaaugagu      240

uaugaauguc caauguuaga ugaaggcgua gaaccagaug acguagacug uugguguaau      300

acuacaagua cuuggguagu cuauggaaca ugccaucaua agaaaggaga agcucgacgg      360

ucacguagag ccguaaccuu accuucucac ucaauaagaa aauuacaaac acgaagucaa      420

acauggcuag aaagcagaga auauacaaaa caucuaauac gagucgaaaa uuggauauuu      480

cgaaauccgg gauuugccuu agccgcggca gcaauagcgu gguuacucgg uaguucaaca      540

ucacaaaaag uaauuuacuu gguaaugaua cugcuuauag ccccagccua cucaauaaga      600

uguauaggag uaaguaauag agauuuuguu gaaggaaugu caggcggaac uugggucgau      660

guaguauuag aacacggcgg uugugugaca guuauggccc aagacaaacc aacaguagau      720

auagaauuag uaacaacuac aguauccaau auggcagaag uaagaaguua uuguuaugaa      780

gcaucaauau cggauauggc auccgauagu agauguccca cacaaggaga agcauaucua      840

gauaaacaau cagacacaca auauguaugu aaacgcaccu uaguagacag agguugggga      900

aacggaugug gauuauuugg aaaaggaagu cucgugacuu gcgcuaaauu ugcauguagc      960

aagaaaauga caggcaaaag uauacagcca gaaaauuuag aauauagaau aauguuaagu     1020

guacauggaa gucaacauag uggaaugauu guaaaugaua caggacauga aacagaugaa     1080

aacagagcaa aagucgaaau aacaccaaac ucaccacgag cagaagccac acuaggagga     1140

uuuggaagcc uaggcuuaga uugugaacca agaacaggac uagacuuuuc agauuuguac     1200

uauuuaacua ugaauaauaa acacuggcua guucauaaag aaugguucca ugacauacca     1260

uuaccauggc acgcuggugc agauacaggg acaccacacu ggaauaauaa agaggcuuug     1320

guagaauuua aagaugcuca cgccaaaaga caaacaguag uaguauuagg aucacaagaa     1380

ggagcuguac auacagcauu agcuggugcg uuagaagcag aaauggaugg agcaaaaggu     1440

cgauuaagcu caggacacuu aaaauguaga uuaaaaaugg acaaauuaag acuaaaaggu     1500

guaucauaua gucuaugcac agcugcauuc acauucacaa aaauacccgc cgaaacacuu     1560

caugguacag ucacagucga aguccaauau gcaggaacug auggaccaug caaaguaccu     1620

gcgcaaaugg cuguagacau gcaaacgcua acuccaguag gaagauuaau aacagcaaau     1680

ccagucauua cugaaucaac ugaaaauagu aaaaugaugc uagaauuaga uccaccauuu     1740

ggugauuccu auauaguaau aggcguaggu gaaaagaaaa uaacucauca uuggcacaga     1800

agugggucaa cuauaggaaa ggcuuucgaa gccacaguaa gaggugcaaa aagaauggca     1860

guauuaggag acacagcaug ggacuuugga aguguaggag gagcauuaaa cagucuaggu     1920

aaaggaauac aucaaauauu uggagcagca uucaaaucau uauucggcgg aaugagcugg     1980

uuuucacaaa uauuaauagg aacccucuua augugguuag gacuaaaugc uaaaaaugga     2040

ucaauuucac uuaugugucu cgcacuagga ggaguccuaa uauuuuuguc gacagcagua     2100

ucagcagaug uagguuguuc uguggacuuu ucaaaa                               2136


<210>  2611
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long CDS opt

<400>  2611
agaaucauca augcuagaaa agaaaaaaaa agacgcgggg cagauacaag uguuggaauu       60

guaggauugu uacuaacaac agcaauggca gcagaaguaa caagacgagg gagugcauau      120

uacauguauc uagauaggaa ugaugcaggg gaagcuauaa gcuuuccaac aacucuaggu      180

augaauaaau gcuauauaca aauaauggac cugggucaua ugugugaugc aacaaugagc      240

uaugaauguc caauguuaga ugaaggagua gaaccagaug acguagacug uuggugcaau      300

acaacaagua cauggguagu auauggaaca ugucaucaua aaaaaggaga agcacaacga      360

agcagaagag cuguaacacu accaagccac ucuacuagaa aauuacaaac cagaucacaa      420

acguggcugg aaucacguga auauacaaaa caucuuauua gaguagaaaa uuggauauuc      480

agaaauccgg gguuugcauu agcagcagcu gcaauagcau gguuacuagg uaguucuacu      540

ucccaaaaag ucauauacuu aguuaugaua uuauuaauag caccagcuua cagcauacga      600

uguauaggcg uuaguaauag ggauuuugua gaagguaugu caggaggcac auggguagau      660

guaguuuuag aacacggcgg augugucacu guaauggcgc aagacaagcc aacaguagac      720

auagagcuug uuacaacaac gguaucaaau auggcagaag ugaggucaua uuguuacgag      780

gcaucaauaa gugauauggc aucagauuca agauguccaa cacaaggaga ggccuauuua      840

gauaaacaaa gcgauacaca auacguaugc aaaagaacac ugguugauag aggaugggga      900

aauggaugug gacuguuugg aaaaggaagu uuagugacau gcgcgaaauu ugccuguucc      960

aaaaaaauga caggaaaaag cauacaacca gagaauuuag aauauagaau aauguuaagu     1020

gugcacggaa gucaacacag uggaaugauu guaaaugaca caggucacga aacagaugaa     1080

aauagagcua aaguggaaau aacgccuaau ucaccaaggg cagaagcaac auuaggagga     1140

uucggaaguu uagggcuaga cugugaacca aggacagggu uagauuuuag cgaucuguau     1200

uaucugacga ugaacaacaa acauugguua guacauaaag aaugguucca cgauauacca     1260

uuaccguggc augccggagc agacaccggc acaccacauu ggaauaauaa agaagcauua     1320

guagaauuua aagacgcaca ugcaaaacgu caaacaguag uaguucuagg aagucaagaa     1380

ggugcaguac acacugcauu agccggagca cuagaggcgg aaauggacgg agcaaaaggu     1440

cgacuuucaa guggucaucu aaaauguaga uuaaaaaugg acaaacuucg acuaaaagga     1500

guaucuuaua guuuaugcac agcagcauuc acauuuacga aaauuccagc agaaacauua     1560

cauggaacag ugacuguaga gguacaguac gcgggaacag auggaccaug uaaagugcca     1620

gcacaaaugg caguugacau gcaaacauug acaccaguag gacgacucau cacagcaaau     1680

cccguaauaa ccgaaucaac ggaaaauuca aaaaugaugu uggaauuaga cccaccauuu     1740

ggggacaguu auauuguaau aggaguugga gaaaaaaaaa uuacucauca cuggcacagg     1800

agcggaucaa caauagguaa agcguucgaa gcaacuguuc gaggagccaa acgaauggca     1860

guauuaggag auacagcaug ggauuuuggu ucaguaggag gagcauuaaa caguuuagga     1920

aaaggaauac accaaauauu uggagcggca uuuaagucau uguuuggagg gaugucaugg     1980

uucucacaaa uacuaauagg aacacuguua augugguuag gauuaaauac caaaaacggc     2040

aguauuaguu uaaugugcuu agcacuagga ggaguguuaa uauucuuaag uacugcugua     2100

agugccgaug uaggcuguag ugucgacuuu agcaaa                               2136


<210>  2612
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long CDS opt

<400>  2612
agaauaauaa acgcaagaaa agaaaaaaag agaagaggag cagauaccag uguagguauu       60

guaggucugu uauugacaac agcuauggca gcagaaguaa caagaagagg aagugcuuau      120

uacauguauu uagauagaaa ugaugcagga gaagcaauau cauucccaac aacauuagga      180

augaauaaau gcuauauaca gauaauggac uuaggacaca ugugugaugc aacuaugucg      240

uaugaauguc caaugcuaga ugaaggagua gaaccggaug auguugauug cugguguaau      300

acaacgucaa cauggguagu uuauggaacc ugucaucaua agaaagggga agcuagacgu      360

ucaagaagag caguaacccu accaucacac agcacgcgaa aauuacaagc aagaucacag      420

acuugguuag aaucacgaga auauacgaaa cacuuaauac gaguagagaa uuggauauuu      480

agaaauccag gauuugcauu agcagcagcc gcuauagcau gguuacuugg aucaucaaca      540

ucccaaaaag uaaucuaccu aguaaugaua cuauuaauag caccagcuua uaguauuaga      600

uguauaggag uaaguaauag agauuuuguu gaaggaauga gugguggaac guggguagau      660

guaguauuag aacauggcgg auguguaacc guuauggcac aagacaaacc aacaguggac      720

auagaacuag uuacaacaac uguaagcaac auggcggaag uaaggucaua uuguuacgaa      780

gcuagcauaa gugacauggc aucagauagu agauguccaa cacaaggcga agccuaucua      840

gauaaacaau cagacacaca auauguaugu aagagaacac uuguagaucg uggaugggga      900

aacgguugug gauuguuugg uaaaggauca cuaguuacuu gugcaaaauu cgcauguagu      960

aaaaaaauga cgggaaaaag cauacaacca gaaaauuuag aguacagaau aaugcugagu     1020

guucaugguu cacaacauuc aggaaugaua guaaacgaua caggucacga aacugaugaa     1080

aacagagcga aaguagaaau aacuccaaau aguccuagag ccgaagcaac uuuaggcggu     1140

uuugguucau uaggauuaga cugugaacca agaacuggau uagauuucuc ugauuuauac     1200

uacuuaacua ugaauaauaa acauuggcua guccauaaag aaugguuuca ugacauacca     1260

uuacccuggc acgcaggugc cgacacaggu acaccacauu ggaauaauaa agaagcauua     1320

guugaauuua aagaugcaca ugcaaaaaga caaacaguag uaguauuagg gagucaagaa     1380

ggagcaguac auacagcuuu agcaggggca uuggaagcag aaauggaugg cgcuaaaggu     1440

agacuaucau cuggacacuu aaaaugucga cuaaaaaugg auaaauuaag acuuaaagga     1500

guaaguuaua gccuaugcac ggcagcguuu acauuuacaa aaauaccugc ugaaacuuua     1560

cauggcacag uuacaguaga aguacaguau gcgggaacag auggucccug uaaaguacca     1620

gcucaaaugg cuguagauau gcaaacguua acaccaguag gcagauuaau aacagcaaac     1680

ccaguuauaa cagaaaguac cgaaaauagc aaaaugaugu uggaauuaga uccaccuuuu     1740

ggagauucau auauaguaau aggaguugga gaaaaaaaaa uaacacauca uuggcauaga     1800

aguggaucaa ccauaggcaa agcuuuugag gcaacaguaa ggggagcaaa acgaauggca     1860

guauuaggag auacagcaug ggauuuuggu ucaguaggag gagcacucaa uagucuuggc     1920

aaaggcauac aucagauauu uggagccgcu uucaaaucau uauuuggugg gaugucaugg     1980

uucucacaaa uucugauagg aacacuguua augugguuag gccuuaauac gaaaaaugga     2040

ucaauuagcu uaaugugcuu agcauuagga ggaguacuaa uuuuuuuauc aaccgcagua     2100

agcgcugaug uagggugcag uguagauuuu ucaaaa                               2136


<210>  2613
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long CDS opt

<400>  2613
agaauaauaa augcacgaaa agagaagaaa agacggggcg cagacacaag ugucggaauu       60

guaggauuac uucuaacaac agcaauggca gcagaaguaa caagaagagg aagugcauau      120

uauauguauc uggauagaag cgaugcagga gaagcaauua gcuucccaac aacguuaggu      180

augaauaaau gcuacauaca aauaauggau uuaggacaua ugugcgacgc aacaaugagu      240

uacgaauguc caaugcugga ugaaggagua gaaccagacg auguggauug uuggugcaac      300

acuacaagua cuuggguggu auauggaacu ugccaucaca agaaagguga agcaagaaga      360

ucaaggagag cuguuacguu accaucacac aguacaagaa aauuacaaac aagaagucaa      420

acauggcugg aaaguagaga auacacaaaa cacuuaauaa gaguugaaaa uuggaucuuu      480

aggaacccag gauuugcacu agcugcagca gcaauagccu ggcuacuagg uaguaguaca      540

agccaaaaag uaauauauuu agucaugauc cuauuaauag caccagcaua cucuauaaga      600

uguauaggag uaagcaauag agauuucgua gaaggaaugu cuggaggaac cuggguagau      660

guaguuuuag aacacggagg cugcguaaca guuauggccc aagauaaacc gacaguugau      720

auagaacuug uaacaacaac gguaaguaau auggcagaag uaagauccua cuguuacgaa      780

gcaaguauaa gugacauggc uucagauuca agauguccaa cacaagggga agcguaccuu      840

gauaaacaau cugauacaca auauguuugu aaaagaaccc uaguagauag agguugggga      900

aauggaugug gacuuuuugg aaaagggagu uuaguuacau gugcaaaauu cgcauguagu      960

aagaaaauga cagguaaaag uauacaacca gaaaauuugg aauacaggau aauguuaagu     1020

guccauggaa gucaacauag cggaaugaua gucaaugaua cggggcauga aacagacgaa     1080

aacagagcaa aaguagaaau aacaccuaau uccccgcgag cagaagcaac auuaggugga     1140

uuuggcucau uaggauuaga uugcgaacca aggacuggau uagauuuuag ugauuuguau     1200

uauuuaacaa ugaacaauaa acauuggcuu guucauaaag aaugguuuca ugauauucca     1260

cuaccauggc acgcaggagc ugauacaggc accccacauu ggaauaauaa agaagcauug     1320

gucgaauuua aagaugcuca ugcaaaaaga caaacgguag uaguauuagg aucacaagaa     1380

ggagcaguac auacagcacu ugcuggugcu uuagaagcag aaauggaugg agcaaaaggu     1440

cgauuaagua guggacauuu aaaauguaga uuaaaaaugg acaaauugag acuaaaaggu     1500

gucuccuaua gcuuaugcac agcagcauuu acauuuacaa aaaucccagc agaaacauua     1560

cauggaacug uaacuguuga aguacaauau gcagguacag auggcccgug uaaaguucca     1620

gcucagaugg cuguugauau gcaaacauua acaccugucg gaagauuaau aacggcaaau     1680

cccguaauca cggaaaguac ugaaaauagu aaaaugaugc uagaauuaga uccaccauuu     1740

ggggacagcu auauaguaau aggaguaggu gaaaagaaaa ucacacauca uuggcauagg     1800

aguggaagca caauaggaaa agccuuugaa gcaacaguaa gaggagcaaa acgaauggcc     1860

gugcuaggug auacagcaug ggauuucgga ucagugggug gagcucucaa uuccuuagga     1920

aaagggauac aucaaauauu uggagcugca uuuaaaaguc uauuuggggg uaugucuugg     1980

uuuagucaaa uauuaauugg aacauuacuu auguggcugg gauuaaacac uaaaaauggu     2040

ucgauaaguu uaauguguuu agcauuaggu gggguuuuaa uuuuucuaag uacagcagua     2100

uccgccgaug uggguuguag uguagauuuu ucaaaa                               2136


<210>  2614
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long CDS opt

<400>  2614
agaauaauaa augcaagaaa agaaaagaaa agaagaggua cagacaccag uguaggaaua       60

guaggauuac uauugacaac agcaauggcu gcagaaguua cacgcagagg aucugcuuac      120

uauauguacc uagauagaag ugaugcagga gaagccauuu cauucccaac aacuuuaggu      180

augaauaaau guuauauaca aauaauggau cuaggacaca ugugcgacgc aacuaugagu      240

uaugaaugcc cuaugcuaga ugaaggagua gaacccgaug auguugauug uugguguaac      300

acaacaagua cauggguagu auacggaaca ugucaucaua agaaaggcga agcaagaaga      360

agcagacgag caguuacauu accgagccau ucaacaagaa aacuacaaac aagaucucaa      420

accugguuag aaagucgaga auauacaaaa cauuuaauua gaguagaaaa cuggauauuc      480

agaaauccag gauuugcuuu agcagcugca gcuauagcau ggcuacuagg aucauccaca      540

ucacaaaaag uuauauacuu aguaaugauu cuauuaauag ccccagcaua uucaauaaga      600

ugcauaggug uaaguaauag agauuucguc gaaggaauga guggaggaac auggguugac      660

guaguacuag aacauggagg cuguguaaca guaauggccc aagauaaacc gacuguagau      720

auagaauuag uaacaacaac aguaaguaau auggccgaag ucagaucaua cuguuaugaa      780

gccagcauaa gugauauggc aagcgauucc agauguccaa cucaagguga ggcauauuua      840

gauaaacaau cggauacaca auaugugugc aaaagaacuc uaguagacag aggaugggga      900

aacggcugug gauuauuugg aaaaggaagu cuaguaacau gugccaaauu ugcaugcuca      960

aagaaaauga cuggaaaauc aauacaacca gaaaaucuag aauaucgaau aauguuaagu     1020

gugcauggaa gucaacacag cggaaugaua guaaacgaua cuggucauga gacagacgaa     1080

aacagagcaa aaguagaaau aacaccuaau ucaccacgag cagaagcaac cuuaggaggc     1140

uucggauccc uaggauuaga uugugaacca cgaacaggcu uagacuuuag ugauuuauau     1200

uauuuaacaa ugaauaacaa acauuggcug guacauaaag aaugguuuca cgauauccca     1260

uuaccauggc acacaggugc agauacagga acaccacauu ggaacaacaa agaggcgcuu     1320

guagaauuua aagaugcuca cgcaaaaaga caaacaguug uaguauuggg aucucaagaa     1380

ggcgcaguac auacagcauu agcuggagcu cuagaagcag aaauggacgg ugcaaaaggc     1440

cgacugucuu cuggucacuu aaaaugcaga cucaaaaugg auaaauuaag acuuaaaggc     1500

guaucauaca guuuauguac cgcugcuuuc acauuuacaa aaauaccagc ugaaacuuua     1560

cauggaaccg uaacaguaga agugcaauac gcugguacug auggaccaug uaaagugccc     1620

gcucaaaugg caguagauau gcagacacua acaccagugg gacgauuaau uacugcaaau     1680

ccaguuauua cagaaggaac agaaaacuca aaaaugaugu uagaauuaga uccaccauuu     1740

ggugauucau auauaguaau aggaguaggu gagaaaaaga uuacacauca uuggcauaga     1800

ucaggaucaa caauagggaa agcguucgaa gcuacaguaa gaggagcuaa aagaauggca     1860

guauuaggag acacggcaug ggauuuugga ucagugggug gaguacucaa uucauuggga     1920

aaaggaauac accaaauauu cggagcagcc uucaaaagcc uauuuggagg aaugaguugg     1980

uuuagucaaa uacucauagg aacauuguua auguggcuag gcuuaaauac uaaaaauggc     2040

aguauaucac uaaugugucu ugcacuggga ggaguacuua uauuucuauc aacagcugua     2100

uccgcagaug uaggauguag cguagauuuu agcaaa                               2136


<210>  2615
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long CDS opt

<400>  2615
agaauuauaa augcaaggaa agaaaaaaaa agaaggggag cugauacauc aguaggaaua       60

guuggucuuc uacuaacuac agcaauggca gcggaaguaa cgcgaagagg aucagcauau      120

uauauguauc uagauagaaa ugaugcagga gaggcaaucu cuuuuccuac aacauuagga      180

augaacaaau guuacauaca aauaauggac uugggacaua ugugcgaugc aaccauguca      240

uaugaauguc cuauguugga ugaaggugua gaaccugaug acguugauug uuggugcaau      300

acaacaucaa cauggguagu auacgggaca ugucaucaca aaaaagguga agcuagaaga      360

ucaagaagag cuguuacauu accuucacac ucaacacgaa aauuacaaac aagaucacaa      420

acaugguuag aaucaagaga auauacaaaa caccuaauaa gaguagaaaa uuggauauuu      480

agaaauccag guuuugcauu agcagcagca gcaauagcau gguuauuagg auccucaacg      540

agucaaaaag uuauuuauuu aguaaugauu uuguuaauug cuccagcuua uucaauaaga      600

ugcauaggug uaaguaaucg agacuuugua gaagggauga guggugggac auggguagau      660

guaguauuag aacacggcgg augcguaacg gcaauggcgc aagacaaacc aacaguagau      720

auagaacuag uuacaacuac gguuucuaac auggcagaag uaagaucuua uugcuacgaa      780

gcaucaauau cagacauggc aucugauagu agauguccaa cacaagggga agcauaucua      840

gauaaacaau cagauacaca auauguaugu aaacgaacuc uaguagaccg uggcuggggu      900

aacggaugcg ggcucuuugg aaaaggauca uuaguaacau gugcaaaauu ugcuuguucc      960

aagaaaauga caggaaaaag cauacaacca gaaaaccuug aauauaggau aauguuauca     1020

guacauggcu cacaacauag ugguaugcuu guaaaugaua caggacauga aacggacgaa     1080

aaccgagcaa aaguugaaau aacuccaaau aguccgcgag cugaagcaac guuaggcgga     1140

uuuggaagcu uagggcuaga uugugaacca agaacaggau uggauuucuc agaucuuuau     1200

uauuuaacaa ugaauaauaa acauugguua gcacacaagg aaugguucca ugauauaccc     1260

cuaccauggc augcaggugc agccacggga acuccucauu ggaacaauaa agaagcauua     1320

guagaauuua aagacgcaca cgccaaaaga caaaccgucg uuguacuugg aucacaagag     1380

ggagcaguac auacagcauu agcaggagca uuggaagcag aaauggaugg ugcuaaagga     1440

agauuaucua gcggacaucu aaaaugcagg cuaaaaaugg acaaacuacg auuaaaaggu     1500

guaagcuaua gucuaugcac agcagcuuuu acauuuacaa aaauacccgc agagacuguu     1560

gacgguacag uaacaguaga aggucaauau ggagggacug augguccuug caaaguucca     1620

gcacaaaugg cagucgacau gcaaacacua acgccaguug guagacuaau aacagcgaau     1680

cccgucauua cagaaucaac agaaaauucu aaaaugaugu uagaauuaga uccaccauuu     1740

ggcgauagcu auauaguuau cggaguaggc gaaaaaaaaa uaacucauca uuggcaccgg     1800

aguggaagca cuauaggaaa agcauucgaa gccacaguaa ggggugcaaa aagaauggca     1860

gugcuaggag auacugcaug ggauuuugga aguguagggg gcgcauuaaa uaguuuagga     1920

aaaggaauac aucaaauaau aggugcagcc uuuaaaaguc uauucggagg aaugaguugg     1980

uuuagccaaa uauuaauagg aaccuuacug augugguuag gacuuaacac gaaaaaugga     2040

agcauaucac uuaugugucu cgccuuggga ggaguauuaa uauuucuauc gacggcagua     2100

agcggcgggg uagguuguuc aguagauuuu ucaaaa                               2136


<210>  2616
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long CDS opt

<400>  2616
agaauaauaa acgcaagaaa agaaaaaaaa aggagaggua cagauacauc cgucggaaua       60

guggguuuau uacuuacaac ugcaauggcg gcagaaguaa cacgaagagg aagcgcauau      120

uauauguauu uagaucgaag ugaugcggga gaagcaauaa gcuucccaac aacauuagga      180

augaacaaau guuauauaca aauaauggau uuaggacaca ugugugaugc aacgauguca      240

uaugaauguc caauguuaga ugaaggagua gaaccggaug augucgauug uugguguaau      300

acaacaucaa cauggguagu auauggaacc ugccaucaua aaaaagguga agcaagaaga      360

ucuaggagag caguaacgcu uccaagucau aguacaagga aauuacaaac uagaagucaa      420

acaugguuag aaaguagaga auacacuaaa cauuuaauac gaguagaaaa cuggauauuu      480

agaaauccag gcuucgcauu agccgcagcu gccauagcau gguuauuagg uucgaguacg      540

ucacaaaaag uuauauauuu aguuaugauu cuauuaauug cccccgcaua uaguauaaga      600

ugcauaggag ugaguaaccg ggauuuuguu gaaggaaugu caggaggaac augggucgau      660

guuguauuag aacauggugg augcguaaca guuauggcac aagacaaacc aacaguggac      720

auagaauuag uaacaacaac gguaaguaau auggcagaag uacgaaguua cuguuaugaa      780

gcaaguauau cagauauggc aagcgauucu agauguccaa cucaaggcga agcauaucua      840

gauaaacaau ccgauacaca auacgugugc aagaggacgu ugguagauag aggauggggu      900

aaugggugcg gguuauucgg aaaagguuca uuaguaaccu gcgcuaaauu cgcguguucu      960

aaaaaaauga caggaaaauc aauacaaccg gaaaauuuag aguauagaau aauguuguca     1020

guucauggaa gucaacauuc aggaaugaua guaaaugaua cuggacauga aacagacgag     1080

aaccgagcaa aaguugaaau aacaccaaau aguccaaggg cagaagcuac auuaggagga     1140

uuuggcucuc uaggacugga uugugaacca agaacuggcu uagauuuuag cgaccuauau     1200

uaccuaacga ugaauaauaa gcacuggcua guacacaaag aaugguucca cgauauacca     1260

uuaccauggc augcaggugc agauacagga acuccacauu ggaauaauaa agaagcacua     1320

guggaguuua aagacgcaca ugcaaaaagg caaacaguug uaguuuuagg cagucaagaa     1380

ggcgcuguac auacagcauu agcaggagca cuagaagcgg aaauggaugg agcaaaagga     1440

agauuaucaa gcggacaucu aaaauguagg uuaaaaaugg auaagcuuag auuaaaagga     1500

gucaguuacu cauuauguac ggcagcuuuu accuuuagca aaauaccagc agaaacacua     1560

cacggcacag uuacugaaga agugcaauau gcuggaacug auggcccaug caaaguaccg     1620

gcacaaaugg caguagauau gcaaacacua acaccaguag gaagauuaau aacagcaaac     1680

ccaguaauaa cagaaucaac agaaaacagu aaaaugaugu uagaauuaga cccaccauuu     1740

ggagauucau auauagucau aggaguagga gaaaaaaaaa uuacccauca uuggcauaga     1800

agugguagua caauaggcaa agcauucgaa gcuacaguaa gaggagcaaa aaggauggca     1860

gucuuaggag acacagcaug ggauuuugga ucaguaggag gagcuuuaaa uaguuuagga     1920

aaaggaauuc aucagauauu cggagcagca uuuaaaagcc uauucggugg aaugagcugg     1980

uuuucgcaaa uauugauagg aacauuacua augugguuag gauuaaacac aaaaaacgga     2040

ucuauaaguc ugaugugccu agcguuaggg ggcguucuaa uauuccucuc aacagcagua     2100

ucagcggacg uaggauguuc aguagauuuc ucaaaa                               2136


<210>  2617
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long CDS opt

<400>  2617
agaauaauaa augcacgaaa agaaaaaaaa agaagaggaa cagacacuuc aguaggcauu       60

guaggacuac uacuuacaac agcaauggca gcagagguaa cacgaagggg aagugcauau      120

uauauguauu uagacagauc agaugcagga gaagcgaucu cauuuccuac aacauuggga      180

augaauaaau guuacaucca aauaauggau cugggccaua ugugugaugc aacaaugagu      240

uacgaauguc caauguuaga ugaagggguc gagccagaug auguagauug uugguguaau      300

accacuucaa cauggguugu uuauggaaca ugccaucaua aaaaaggaga agcaagaaga      360

uccagaaggg caguaacauu accaucacau ucuacacgaa aauuacaaac ccgaucgcaa      420

acauggcuag aaaguagaga auacaccaaa cauuuaauaa gagucgaaaa uuggauauuc      480

agaaacccag gcuuugcuuu agcagcagcu gcgauagccu ggcuacuagg aaguagcacu      540

ucacaaaaag ucaucuauuu aguaaugauu uuacuaaucg cuccagcaua cagcauuaga      600

uguauuggag uuaguaauag agacuuugua gaaggaaugu caggcggaac auggguugau      660

gugguccuag aacauggcgg auguguaaca guaauggccc aagauaaacc cacaguagau      720

auagaauuag uaacgacaac agugagcaau auggcagaag uaagaucaua uugcuacgaa      780

gcuucaauau cugauauggc aagcgauucg agaugcccaa cacaaggaga agcauaucuc      840

gauaaacaaa gcgacacuca auauguaugc aaaagaacac uaguagacag aggcuggggu      900

aaugguugug guuuguuugg aaaagggagu uuaguaacau gugcuaaauu cgcaugcucg      960

aagaaaauga caggcaaauc aauacaaccu gaaaauuuag aauacagaau aaugcuaagu     1020

guacaugguu cacaacauuc agguaugauu guaaaugaua caggucacga aacagaugaa     1080

aauagagcua aaguagaaau aacccccaau ucaccaagag cugaagccac acuaggaggc     1140

uucgguaguu uaggguuaga uugugaaccu agaacagggc uugauuuuuc agaccuuuau     1200

uaucucacaa ugaauaauaa acacuggcuc guacauaaag aaugguucca ugacauaccu     1260

cuacccuggc acgcuggagc agauacagga acgccacacu ggaauaauaa agaagcuuua     1320

guagaauuca aagaugccca cgccaaaaga caaacuguag uaguacuagg aagucaagaa     1380

ggagcaguac acacagcuuu agcaggagca uuagaagcgg aaauggacgg agcgaaagga     1440

agauuaucau cuggucauuu aaaauguaga uugaaaaugg auaaacuaag auuaaaaggu     1500

guuucauaua gcuuaugcac agcugcauuu acauuuacaa aaauaccagc agaaacuuua     1560

cauggcacag uaacaguaga aguccaguau gcagguacug auggaccuug uaagguaccc     1620

gcacaaaugg caguagauau gcaaacacuc acaccaguag gaagacuaau uacagcuaau     1680

ccaguuauua cagaaagcac agaaaauucc aaaaugaugc uagaguuaga uccaccuuuu     1740

ggugacaguu auauagugau aggggucgga gaaaaaaaaa uaacacauca cuggcauaga     1800

agcggaucaa caauaggaaa agcauuugaa gccacaguaa gaggagcuaa aagaauggcu     1860

guacuaggcg auacagcaug ggauuucgga aguguuggcg gagcucugaa cagccucggg     1920

aaagguauac aucaaauauu uggugcggca uuuaaaaguu uauuuggagg aaugucaugg     1980

uuuucacaaa uauuaauagg aacacuacua augugguuag gucucaauac uaaaaaugga     2040

aguauaagcu uaauguguuu agcauuggga ggagucuuaa uauuccuauc aacagcagua     2100

agugcagaug uaggauguag uguugauuuu uccaaa                               2136


<210>  2618
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long CDS opt

<400>  2618
agaaucauua augcacguaa agaaaagaaa agaaggggaa cagauacguc uguaggaaua       60

guuggacuac uauuaacaac ugcaauggcu guagaaguaa caagaagagg aaaugccuau      120

uauauguauu uagauagguc ggacgcagga gaagcaauuu cauucccaac aacaauggga      180

augaacaaau guuauaucca aaucauggac cuuggacaua ugugugacgc aacaauguca      240

uaugaauguc caauguuaga ugaagggguu gaaccagaug auguagauug uuggugcaac      300

acaacaagca cauggguagu cuacggaaca ugucaucaua agaaaggaga agcacgaaga      360

ucaagacgag caguuacauu accaagucac ucaacaagaa aauuacaaac aagaucacaa      420

acauggcucg aaaguagaga auacaccaaa caucugaucc gaguagaaaa cuggauauuu      480

agaaaucccg guuuugcucu ugcugccgcc gcaaucgcau gguuacuagg aucgucuacu      540

ucacaaaaag uuauauauuu aguaaugaua uuauugauag cuccagcaua uuccauuaga      600

ugcauaggag uuuccaauag agauuuugua gaagggauga gcggagguac cuggguagau      660

guaguauuag aacacggagg augcguuaca guaauggcac aagacaaacc aacaguagac      720

auagaauuag uuacaacaac aguaucaaac auggcagaag uucguuccua uugcuacgaa      780

gcaaguauuu cagauauggc aagugauagu agauguccga cacaaggaga agcauauuua      840

gacaaacaau cagacacuca auauguaugu aaaagaacuc uaguagauag agguugggga      900

aauggcugug gauuauucgg caaaggaucu cuaguaacau gugcaaaauu ugccuguagu      960

aagaaaauga caggaaaauc cauccaacca gaaaauuuag aauaucgaau aauguuauca     1020

guacauggau cacaacauuc cggaaugauc gucaacgaua cuggucauga gacugaugaa     1080

aauagagcaa aaguagaaau aacaccaaau ucaccucgag cugaagcaac auugggagga     1140

uuuggaucac uaggacuaga cugugaacca agaacaggac uugauuuuag cgacuuauau     1200

uaccuuacga ugaacaauaa acauugguua guacauaaag aaugguuuca cgacauacca     1260

cuaccuuggc augcaggagc agauacggga acuccccauu ggaacaauaa agaggcuuua     1320

guagaguuca aagacgcaca cgcaaaaaga caaacaguag uaguucuugg aagucaagaa     1380

ggagcuguac acacagccuu agcuggugca cuagaagcag aaauggaugg agcaaaagga     1440

agauuaagua guggacacuu aaaauguaga uuaaaaaugg auaaauuacg auuaaaagga     1500

guuucauauu cauuaugcac agcagcuuuu acguucacga agauaccagc agaaacacug     1560

cacggaacag uaacaguaga aguacaauau gccgguacag auggaccuug uaagguacca     1620

gcacaaaugg cuguugacau gcaaacauua acucccguug guagacuaau aacagcaaau     1680

ccaguaauaa cugaaagcac agaaaauucu aaaaugaugc uagaauuaga uccaccuuuu     1740

ggagauagcu acaucguaau aggaguuggc gaaaagaaaa uaacucauca cuggcaccgu     1800

ucagggucaa ccauugguaa agcauuugaa gcaacaguaa gaggagccaa aagaauggcg     1860

guacuaggag auacggcaug ggauuuugga aguguaggag gugcccuaaa uuccuugggu     1920

aaaggaauac accaaaucuu uggagccgca uuuaaaucau uauucggggg aaugaguugg     1980

uucagccaaa uauuaauugg aacacuauua guaugguuag gauugaauac uaaaaaugga     2040

ucuauaucac uaaugugccu agcauuagga ggagugcuca uauuccuaag uacugcuguu     2100

ucagcagaug uaggauguag uguagauuuc agcaaa                               2136


<210>  2619
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long CDS opt

<400>  2619
agaauaauaa augcaagaaa agaaaaaaaa cgaagaggaa cagauacaag cguggguaua       60

guuggcuugc uauugacuac ugcaauggca gcugaaguaa ccagaagagg aagugcauac      120

uacauguauc uagaucguuc ugaugccggu gaagcaaucu cauuuccuac aacauuaggu      180

augaacaaau guuauauaca aauaauggau cuaggucaua ugugugaugc aacaaugucu      240

uaugaauguc cuaugcugga cgagggaguu gaaccagaug acgucgauug cuggugcaau      300

accacgucca cauggguagu uuauggaacu ugucaccaca aaaaaggaga agcaagaaga      360

aguagaagag caguaacgcu accaagucau aguacaagaa aacuccaaac aaggucacag      420

acuuggcucg aaaguaggga auauacuaaa caucuaauac gaguagaaaa uuggauauuu      480

agaaauccag gguuugcguu agcagcagca gcaauagcuu gguuauuagg cuccaguacu      540

ucacaaaaag uaaucuacuu aguaaugaua uuauuaauag ccccagcaua uucaauaaga      600

uguauaggug uuaguaauag agauuuugua gaaggaaugu caggagguac uuggguagau      660

gucguucuag aacacggagg augcguaaca guaauggcac aagauaaacc cacuguagau      720

auagaacuag uuacaacaac uguauccaau auggcagaag uaagaaguua uuguuacgaa      780

gcuagcauaa gcgauauggc aagugacuca agauguccca cacaagguga agcauaccuu      840

gauaaacaau cagauacuca auauguaugc aaacguacac ugguagaccg uggauggggg      900

aauggaugcg gacuauucgg uaaaggaucc uuaguuacau gugcaaaauu ugcuuguuca      960

aaaaaaauga cugggaaauc aauacaacca gaaaacuuag aauauagaau uauguuaucg     1020

guacacggau cacagcauuc uggaaugaua guaaaugaua caggacauga aacagaugaa     1080

aaucgagcua aaguagaaau aacuccuaau aguccaagag cggaagcaac ucuaggaggu     1140

uuuggaaguc uaggauuaga uugcgaaccg agaacugggu uagacuuuuc agauuuauau     1200

uaucuaacua ugaacaacaa gcacugguua guacauaaag agugguuuca ugauauaccg     1260

cuaccauggc augcgggagc ugauacagga acuccacauu ggaauaauaa agaagcacua     1320

guggaauuua aagaugcaca ugcaaaacga caaaccgugg uaguacuagg aucacaagaa     1380

ggagcaguac auacagccuu agcuggugca uuagaagcag aaauggaugg agcaaaagga     1440

agauuaagua guggucaucu aaaauguaga cuaaagaugg acaaauuaag auuaaaggga     1500

gugucuuaca gucuauguac agcagccuuu acauuuacaa aaauaccugc ugaaacauua     1560

cacggaacag uaacugugga aguacaauau gcagguaccg auggaccaug uaaaguuccu     1620

gcacaaaugg cuguagauau gcaaacucuc acaccgguag gacgacuaau aacagcaaau     1680

ccaguaauca cagaaaguac ugaaaauagu aaaaugaugc ucgaguuaga uccacccuuu     1740

ggcgacagcu auauaguaau cggaguaggu gaaaaaaaaa uuacacacca cuggcacagg     1800

aguggaagca caauaggaaa agcuuucgaa gcaacaguua gaggugcaaa aagaauggcu     1860

guacugggag auacagcaug ggacuuugga aguguaggag gagcacuaaa uagcuuagga     1920

aaaggaauac accaaauauu uggagcagca uuuaaaucac uauuuggagg uaugaguugg     1980

uuuucgcaaa uuuuaauagg aacauuauua augugguuag ggcuaaauac aaaaaaugga     2040

aguauaucau uaguuugucu agcgcucgga ggaguacuua uauuuuuaag cacagcagua     2100

ucagcagacg uuggauguag uguggauuuu agcaaa                               2136


<210>  2620
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long CDS opt

<400>  2620
agaauaauaa augcaagaaa agaaaaaaaa agaagaggaa cagacacaag uguuggaauu       60

guugguuuac uacuuacgac agcuauggca guagagguua caaggagagg uagugcauau      120

uauauguacc uagauagauc agaugcagga gaagccauau cauuuccgac cacuuuagga      180

augaauaaau guuauauaca aauaauggau cuaggacaua ugugugaugc aacaaugagu      240

uacgaaugcc caauguuaga ugaaggagua gaaccugaug auguagauug cugguguaac      300

acuaccucaa cauggguugu auacggaaca ugucaucaua aaaaagguga agcuagacga      360

agcagacgag cuguaacguu accaagucau ucaacacgaa aacuacaaac uagaucacag      420

acauggcuug aaucaagaga auauacaaaa cacuuaauua gaguugaaaa uuggauauuu      480

agaaauccag gauuugcacu cgcagcagca gcaauugcau ggcuccuugg caguaguaca      540

ucacaaaaag uaauauauuu aguuaugaua uuauuaauag cuccagcaua caguauucga      600

ugcauaggag uuucaaacag agacuuugua gaaggaaugu caggaggcac auggguagac      660

guaguacuag aacacggcgg auguguuaca guuauggccc aagauaaacc agccguagau      720

auugaauuag uuacuacgac aguaaguaau auggcagaag ugagaucaua uuguuaugaa      780

gcaaguauau cagauauggc gucagauagc cguugcccca cccaaggcga agcuuaucua      840

gacaaacaaa gugacacaca guacguaugu aaaagaacau uaguagacag gggaugggga      900

aauggaugcg gacuauuugg caaaggaagc uuaguaacau gcgcaaaauu cgcuuguagc      960

aaaaaaauga caggaaaauc aauacaacca gaaaacuuag aauauagaau uauguuguca     1020

guacacggga gucaacauuc cggaaugaua guaaaugaca cuggucacga aacagacgaa     1080

aauagagcaa aaguagaaau aacgccaaau ucuccuagag cagaagcaac acuaggagga     1140

uuugggucuu uaggguuaga cugugagcca agaacaggau uagauuuuuc ugauuuauau     1200

uauuuaacaa ugaauaacaa acauugguua guacauaaag agugguucca ugacauaccu     1260

cuaccauggc augcaggagc agauacugga acuccacacu ggaauaacaa agaagcacuu     1320

guugaauuua aagacgcuca ugcaaaaaga caaaccguag uuguauuagg aucacaagaa     1380

ggugcggugc acacagcuuu agcaggggcc cuagaagccg agauggaugg ugcaaaaggu     1440

agacuuagcu cuggucaccu uaaaugccga cuaaaaaugg auaaauuaag acuaaaggga     1500

guaaguuaua gccuauguac cgcagcguuu acauuuacaa aaauaccagc agaaacacua     1560

cauggaaccg ugacaguaga aguucaauac gcaggaacag augguccaug uaaaguacca     1620

gcacaaaugg caguagauau gcaaacauua acaccagugg gaagauuaau aacagcaaac     1680

ccaguaauaa cagaaaguac agaaaauuca aaaaugaugu uagaauuaga ccccccuuuu     1740

ggagauagcu auauagucau agguguuggc gaaaaaaaaa uaacacauca uuggcauaga     1800

agcgguucaa caauaggaaa ggcauuugaa gcaacgguac gaggugcgaa aaggauggcu     1860

guauuaggag auacggcaug ggauuuugga ucaguaggag gagcguuaaa uucauuaggc     1920

aaaggaauac aucaaauauu cggagcugcg uuuaaaucau uauuuggugg uaugucaugg     1980

uucagucaaa uauuaauagg aacacuuuua gucuggcuag guuuaaacac caaaaauggu     2040

agcauaucau uaacaugcuu agcauuagga ggaguauuaa uauuccucag cacagcgguu     2100

ucagcagacg uaggauguag uguagauuuu aguaaa                               2136


<210>  2621
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long CDS opt

<400>  2621
agaauaauaa augccagaaa agaaaaaaaa agaagaggag cggacacuuc aguagguaua       60

guaggacuau uauuaacaac agcgauggcc guagaaguaa caagaagagg aagugcauau      120

uauauguacu uagauagaag cgacgcagga gaagcgauca guuucccaac aacauuaggu      180

augaauaaau guuauauaca aauaauggau cuuggacaua ugugugacgc aacuauguca      240

uacgaauguc caaugcuaga cgaaggagua gaaccugaug acgucgauug uugguguaau      300

acaacuagca cauggguagu auauggaaca ugucaucaua aaaaggguga agcuagaaga      360

aguagaagag caguaacgcu uccaucacac aguacaagaa aauuacaaac uagaagucaa      420

acauggcuag aaucuagaga auauacuaaa caucucauac gaguagaaaa cuggauauuu      480

cguaauccgg gauuugcauu ggcugcggcu guaauagccu gguuauuagg aaguaguaca      540

agucaaaaag ugauauauuu aguaaugaua cuacuuauug cuccagcuua caguauaaga      600

ugcaucggag uaucaaauag agauuucguc gaaggaauga guggagggac auggguagac      660

guaguacuag aacauggagg cugcguaacc guaauggcac aagauaaacc aacaguagau      720

auagaauuag uaacaacgac aguuuccaau auggcugaag uacgaucaua cuguuaugaa      780

gccagcauua gugacauggc aucugauucu agguguccca cucaagguga ggcauaucua      840

gacaaacaau cagauacaca auauguaugu aaaagaacau uaguagauag aggauggggg      900

aauggaugug ggcuauuugg aaaaggaucg uuagucacau gcgcgaaauu ugcuuguuca      960

aaaaaaauga caggcaaauc aauucaaccu gagaauuuag aauauagaau cauguuauca     1020

guucauggau cacaacacag cggaaugaua guaaaugaua cgggacauga aacugacgaa     1080

aauagagcaa aaguugaaau aacuccaaau uccccuagag cagaagcaac acuaggugga     1140

uuuggaucau uaggacuaga uugcgaacca agaacaggau uagauuuuag cgaucuauac     1200

uauuuaacaa ugaauaacaa acauugguua guacauaaag aaugguuuca ugauauacca     1260

uuaccauggc augcaggggc agacacuggu acaccgcauu ggaauaauaa agaagccuua     1320

guagaauuua aagacgcaca ugcaaaaaga caaacaguug ucguauuagg uucucaagaa     1380

ggagcaguac auacggcuuu agcaggagcu cucgaagcug agauggacgg agcaaaaggc     1440

agacuuucaa guggacaucu caaauguagg uuaaagaugg auaaacuucg auuaaaagga     1500

guaaguuacu cccuauguac ugcagcauuu acuuuuacaa aaauaccagc ugaaacuuug     1560

cauggaacag uaacaguaga aguacaauac gcaggaacgg acggaccaug caaaguucca     1620

gcacaaaugg caguagauau gcaaacacug acgccaguag gaagguuaau uacagcgaac     1680

ccaguaauua cagaaucuac cgaaaauuca aaaaugaugu uagaauuaga ccccccuuuu     1740

ggagacucuu acauaguuau cggaguaggu gaaaaaaaaa uaacacacca uuggcaucgu     1800

agugguucca caauaggaaa agcuuucgaa gcaacuguaa ggggagcaaa aagaauggca     1860

guuuuaggag acacggcaug ggacuucgga aguguaggag gggcacugaa uucucuagga     1920

aaaggcauac aucaaauauu uggugccgca uuuaaaucau uauuuggagg caugaguugg     1980

uuuucucaaa uauuaauugg uacauuauua guauggcuag gauugaauac caaaaacgga     2040

uccauaucac uaacaugucu agcacuugga ggaguucuaa ucuuucuauc aacagcugua     2100

ucagcagaug uaggcuguuc uguagauuuu agcaaa                               2136


<210>  2622
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long CDS opt

<400>  2622
agaauuauua augcaagaaa agagaaaaaa agaagaggag cagauacaag uguuggaaua       60

guaggauuac uacuaacaac agcaauggca guggaaguaa cacgcagagg aucugcauau      120

uauauguauc uagauagaag ugaugcugga gaagcaauaa guuuuccgac aacucuagga      180

guaaacaaau guuauauuca aauaauggau cuaggacaca ugugcgaugc aacuauguca      240

uacgaguguc cuauguuaga ugaagguguc gaaccugaug auguagacug uugguguaau      300

acaacaucca cguggguagu cuauggcaca ugucaucaua aaaaaggaga agcuagacga      360

ucaagaagag caguuacucu accaagccac ucgacaagaa aacuacaaac aagaucacaa      420

acauggcuug aaucaagaga auauacaaaa caucuaauaa gagucgaaaa uuggauauuc      480

agaaauccag gauuugcauu agcagccgca gcaauagcau gguuauuagg cucaaguaca      540

agccaaaaag uaauauaucu aguaaugaua cucuuaauag caccagcaua cucuauaaga      600

ugcauuggug uuaguaacag agacuuuguc gaaggaaugu cgggaggaac uuggguagau      660

guaguacuag aacauggugg auguguaaca guaauggcac aagauaaacc aacaguagau      720

auagaauuag uuacaacaac gguaucaaau auggcagaag uuagaagcua cuguuaugaa      780

gcaaguauuu cagacauggc aucagacuca agauguccua cacaaggaga agcauaucuu      840

gauaaacaaa gcgauacaca guacguaugu aaaagaacac uaguugauag aggauggggu      900

aauggaugug gucuauuugg aaaaggaagu uuaguaacuu gugcaaaauu ugcauguucu      960

aaaaaaauga caggaaaauc aauucaaccu gaaaacuuag aauauagaau aauguuaucu     1020

guacauggau cccaacauuc aggaaugauu guaaaugaua caggucauga aacagaugaa     1080

aauagagcua aaguagaaau cacuccgaac ucaccacgag cagaagcuac uuuaggagga     1140

uucgggucau uaggccuuga uugcgaacca aggaccggac uagauuuuuc agauuuauac     1200

uacuuaacua ugaauaauaa acacugguua guacacaagg aaugguuuca ugacauaccc     1260

uuaccauggc augcaggagc cgacacagga acuccacauu ggaauaauaa agaagcacua     1320

guagaauuca aagaugcuca ugccaaaaga caaacaguag uaguuuuagg aagccaagaa     1380

ggcgcugucc auacagccuu agcaggagca cucgaagcag aaauggaugg agccaaagga     1440

agacuaucau caggucauuu aaaaugucgu cuuaaaaugg auaaauuaag acuuaaaggu     1500

guuaguuaua gcuuauguac agcggcauuu accuuuacaa aaauaccugc cgaaacacuu     1560

cacggaacag uuacaguuga aguacaauau gcagggacag acggaccaug caaaguuccu     1620

gcacaaaugg caguugauau gcaaacauua acaccaguag gcagauuaau aacagcaaac     1680

ccaguuauaa cugaaucaac cgaaaacagu aaaaugaugu uagaauuaga uccaccuuuu     1740

ggcgacaguu auauaguaau aggaguaggu gacaaaaaaa uaacucauca cugguacaga     1800

aguggaucaa cuauagggaa agcauuugaa gcaacaguaa gaggggcaaa aagaauggcc     1860

guauugggag auacggcaug ggacuuuggc ucaguaggcg gugcuuugaa uaguuuagga     1920

aaagguauac aucaaauauu uggagcagca uuuaaaucau uguuuggagg aaugaguugg     1980

uuuucucaaa uuuugauugg uacauuauua gugugguuag gacuaaauac caaaaacgga     2040

aguaucagcc uaacuugcuu agcccuaggg gggguacuaa uauuuuuaag uacagcagug     2100

agugcggacg uaggaugcag ugucgacuuu ucaaaa                               2136


<210>  2623
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long CDS opt

<400>  2623
aggauaauca augcaagaaa agaaaaaaaa agaagaggug cugacacauc cgucggaaua       60

guaggacugu uacuaaccac cgcgauggcu guagaaguaa cacgacgcgg cagcgcauau      120

uauauguacu uagauagaag ugaugcaggu aaagcaaucu ccuuuccaac aacauuagga      180

guaaauaaau guuacauaca aauaauggau uuaggacaua ugugugaugc aacaauguca      240

uaugaauguc cuaugcugga ugaaggagua gaaccugaug acguugauug uuggugcaau      300

accacuucaa caugggucgu auauggaaca ugucaucaua aaaaaggaga agcuagaaga      360

agcagaagag cgguuacucu uccaagucau aguacuagaa aacuacaaac aagaucucaa      420

acuuggcuug aaucaaggga auacaccaaa cauuuaauaa gaguagaaaa uuggaucuuc      480

agaaauccag gauucgcacu agcagcagcg gcaauagcau ggcuacuugg uucaucuaca      540

agccaaaaag uaauauauuu agucaugaua cuauuaauag cacccgcaua uucuaucaga      600

ugcauaggag uaaguaauag agauuuugua gagggaaugu ccggaggaac auggguagau      660

guaguccucg aacauggugg auguguuaca gugauggcac aagacaagcc aacaguagau      720

auagaauuag uuacgacaac ugucucaaau auggcagaag uaagaagcua uuguuaugaa      780

gcaagcauau cugauauggc aagcgauuca agauguccua cucaaggaga agcguauuua      840

gauaaacagu cagacacaca guacguaugu aaacgaacac uaguagacag aggaugggga      900

aacggaugug gauuauucgg aaaagguagu uuaguaaccu gugcaaaauu ugcguguuca      960

aaaaaaauga cuggaaaaag uauacaacca gaaaauuuag aauauaggau aaugcucagc     1020

gugcauggau cacaacauuc aggaaugaua guaaacgaua uaggacauga aacagacgaa     1080

aaucgagcaa aagucgagau aacacccaau ucaccacgag cagaggcuac ccuuggaggu     1140

uucggaucau uaggauugga uugcgaacca cgaacaggac ucgauuuuag ugauuuauau     1200

uaucuuacaa ugaacaacaa acauugguua guacauaaag aaugguuuca ugacauucca     1260

cuaccauggc augcaggagc agauacuggu acaccacacu ggaacaauaa agaagcacua     1320

guagaauuua aagaugcuca cgcuaaaaga caaacaguag ugguauuagg aagucaagaa     1380

ggagcaguac auacagcacu agcaggagcc cuggaagccg aaauggaugg agcaaaagga     1440

aggcuaucau caggccaucu aaaaugcaga cuaaaaaugg auaaauugag auuaaaagga     1500

guaucguaua gcuuaugcac agcagcguuu acuuuuacaa aaauaccagc ugaaacacua     1560

cacggaaccg uaacuguaga gguacaauac gcaggaacag augguccaug uaaaguccca     1620

gcacaaaugg caguagauau gcaaacauua acacccguag gaaggcuuau aacugcaaau     1680

ccaguuauaa cagaaaguac agaaaauucu aaaaugaugc uggaauuaga uccacccuuc     1740

ggagauucau acauaguaau aggaguagga gauaaaaaaa uuacacauca uuggcauaga     1800

ucugggagua caauugguaa agccuuugaa gcaacagugc gaggcgcaaa aagaauggca     1860

guauuaggag auacagcuug ggauuuugga ucagugggag gagcauuaaa uaguuuagga     1920

aaaggaauac aucaaauauu cggagcagca uuuaaaaguc uauuuggagg caugucaugg     1980

uuuucacaaa uucuuauagg aacgcuuuua guaugguuag gauuaaauac aaaaaaugga     2040

ucaauaucac ugacauguuu agcgcuaggu ggcguuuuaa uauuuuuaag cacggcagua     2100

ucagcagacg uaggaugcuc aguagauuuu uccaaa                               2136


<210>  2624
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long CDS opt

<400>  2624
agaauaauaa augcaagaaa agaaagaaaa agaagaggag cagauacaag cauagguaua       60

guagggcuau uauuaacaac ugcuauggca gcagaaauca cgagaagagg aagugcauau      120

uacauguauu uggacagauc ugaugcagga aaagcaauau cuuucgcaac aacuuuaggc      180

guaaacaaau gucauguuca aauaauggac uuaggccaca ugugugaugc gacaaugagu      240

uaugaauguc caauguuaga ugaaggagua gagccagaug auguugauug uuggugcaau      300

acaacaagca caugggucgu uuacgggacu ugucaucaua agaaaggaga agcaagaaga      360

aguagacgag cuguuacuuu accaagccau aguacaagaa aauugcagac aagaagccaa      420

acauggcucg aaagcagaga auauacaaaa cauuuaauaa aaguagaaaa uuggauauuc      480

cgaaacccag gauuuacgcu gguagcagua gccauagcuu gguuguuagg auccuccacg      540

ucacaaaaag ugauauacuu agugaugaua uuacuaauug caccagcuua uucaauaagg      600

uguauaggag uaucaaauag agauuuugua gaaggaauga guggaggaac uuggguagac      660

guaguacuag aacauggagg uugugugacu guaauggcac aagauaaacc gacuguugau      720

auagaacuug uaacaacaac uguaucaaau auggcugaag uaagaucaua cugcuaugaa      780

gcaucuauaa gugauauggc cuccgauuca aggugcccaa cacaagggga ggcuuacuua      840

gauaaacaaa gugauacaca auauguaugu aaacgaacau uaguagauag aggauggggu      900

aaugguugcg gauuauuugg aaaaggaagu uugguaacau gugcuaaauu uacuugcagu      960

aagaaaauga caggcaaaag cauacaaccg gaaaaucugg aauauagaau aauguuauca     1020

guacauggau cacaacauag uggaaugaua guaaaugaca cugggcauga aacagaugaa     1080

aauagagcaa aaguagaagu uacaccgaac agcccaagag cggaagcaac uuuagguggg     1140

uucggauccc uaggacuaga cugugaacca agaacaggcu uggauuuuag ugacuuauau     1200

uaccuaacaa ugaacaauaa acauuggcua guucauaaag aaugguuuca cgauauacca     1260

uuaccauggc acgcaggagc agauacagga acaccacauu ggaauaauaa agaagcgcua     1320

gucgaguuca aagaugcaca cgcaaaacga caaacaguag ucguacuugg uucacaagaa     1380

ggagcaguuc acacugcuuu agcgggugca uuagaagcag aaauggaugg agcaaaagga     1440

agacuauuuu caggacaucu uaaaugccgu cuuaaaaugg auaaauuaag acuuaaagga     1500

guaaguuauu cacuaugcac agcagcauuc acauuuacua aaguuccugc agaaacauua     1560

cacggaacag ugacaguuga aguccaauac gcuggaacag augguccaug uaagguccca     1620

gcacaaaugg cgguagauau gcaaacauug acaccaguug gaagacuaau uacagcaaau     1680

ccaguaauaa cagaaaguac agaaaauagu aaaaugaugu uagaauugga cccacccuuu     1740

ggagauaguu auauaguuau aggaguagga gauaagaaaa uaacacauca cuggcauaga     1800

ucuggaucaa cgauaggaaa agcauuugaa gcaacuguaa gaggagcuaa aagaauggca     1860

guacuuggag auacagcaug ggacuucggc ucaguaggcg gugucuucaa uucauuagga     1920

aaaggaauac aucaaauauu uggcgcagca uuuaaaucac uauucggagg uaugaguugg     1980

uuuucacaaa uacuuauagg aacccuuuua guuuggcuag gacuaaauac uaaaaauggu     2040

ucaaucaguu uaacauguuu ggcauuagga ggaguuauga ucuuucuaag cacagcuguu     2100

agugcugaug uaggguguag cguagauuuu ucaaaa                               2136


<210>  2625
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long CDS opt

<400>  2625
agaauuauaa augcaagaaa agagagaaag agacgaggag ccgacacauc uauaggaaua       60

auaggacuac uauuaacaac cgcaauggca gcagaaauaa cucgaagagg gagugcauac      120

uacauguauu uagaucgauc agacgcuggu aaagccauaa guuuugcgac uaauuuagga      180

guaaacaaau gccauguuca aauuauggac cuaggacaua ugugcgaugc aacaaugagu      240

uacgaauguc caaugcuaga ugaaggagua gaaccagacg auguagauug cugguguaau      300

acaacaucaa cauggguugu auacggaaca ugucaucaua aaaaaggaga agcaagacga      360

ucaagaagag caguaacuuu gcccucucac agcacaagaa aauuacagac ccgaagccaa      420

accuggcuag aaucaagaga auauacaaaa caucuuauaa aaguugaaaa uuggauauuu      480

agaaaucccg guuuugcguu agcagcagua gcuauagccu gguuacuugg aagcaguaca      540

ucacaaaaag uuauauauuu aguuaugaua uuauuaauug cgccagcuua uucaauuaga      600

uguaucggag uaagcaaccg ggauuucguu gagggaaugu caggggguac auggguagau      660

guaguauuag aacauggggg augcguaaca guaauggcgc aagauaaacc aacaguagac      720

auagaauuag uaacaacgac cgugucaaac auggcagaag uucgcucaua uuguuaugaa      780

gcaaguauau cugauauggc aucagacuca agauguccua cacaagguga agcauacuua      840

gacaaacaau cugauacuca guacgucugu aaacgaacuc uaguagacag aggaugggga      900

aauggaugcg gucuauuugg uaaaggauca cuagugacuu gugcaaaauu uacauguagc      960

aaaaaaauga cagguaaaag uauucagccc gaaaaccuug aguauagaau aauguuauca     1020

guccauggau cucaacauag ugguaugaua gucaaugaua uaggccauga aacagaugaa     1080

aauagagcaa aaguugaagu aacaccaaau ucaccaagag cagaagcaac ccuaggggga     1140

uuuggaucac uuggauuaga uugugaaccu agaacuggac uagacuuuuc agacuuauac     1200

uauuuaacaa ugaacaauaa acauugguug guacauaaag aaugguuuca cgauaucccu     1260

uuacccuggc augccggagc cgauacagga accccacauu ggaauaauaa agaggcacua     1320

guugaauuua aggaugcaca ugcgaaaaga caaacaguag uaguacuagg aucacaagag     1380

ggagccguac auacggccuu agcuggagca cuagaagcag aaauggacgg agcuaaagga     1440

agacuauuua gcgggcacuu gaaaugccga uuaaaaaugg auaaauuacg acuuaaagga     1500

guuuccuacu cauuaugcac agcugcauuu acguuuacua aaguaccugc agaaacauug     1560

cacggaacag uaacaguaga aguacaauau gcugguacag auggacccug uaaagugcca     1620

gcgcaaaugg cuguggauau gcaaacauua acuccuguug gaagacuaau aacagcaaau     1680

ccaguaauaa cagaaucaac ugaaaacagu aaaaugaugu uagaauuaga cccuccauuu     1740

ggggauucgu auauaguaau agguguugga gauaaaaaaa uaacacauca uuggcauaga     1800

agugggucga caauaggaaa agcuuucgaa gcaacaguaa gaggagcaaa aagaauggca     1860

gucuuaggag acacagcaug ggauuuugga ucaguuggag gugucuucaa uaguuuaggu     1920

aaaggaguac accaaaucuu cggagcagcu uucaaaaguc uauuuggggg aaugucaugg     1980

uuuucacaaa uacuuauugg aacauuacua gucugguuag gacucaauac aaaaaauggc     2040

aguauaagcu uaacauguuu ggcauuagga ggcgugauga uuuuccuaag cacagcagua     2100

ucagcagacg uagguuguag uguugauuuc aguaaa                               2136


<210>  2626
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long CDS opt

<400>  2626
cgaauaauaa acgcaagaaa agaacgaaaa agaagaggag cagacacauc aauuggaaua       60

auaggucugu uauuaacaac agccauggcc gcagaaauua caagaagagg cagugcauau      120

uauauguauu uagaccgaag ugaugcugga aaagcaauau cauucgcuac uacauuggga      180

gucaauaaau gucaugucca aauuauggac cuuggucaua ugugcgacgc aacuauguca      240

uaugaauguc cgauguuaga ugaggguguu gaaccagacg acguagauug uuggugcaac      300

acaacaagua cuuggguagu auauggaaca ugccaucaua aaaaaggaga ggcacgccga      360

aguagaagag caguaacacu accaucucac ucaacaagaa aauuacaaac uaggucacaa      420

acaugguuag agucacgcga auacacaaaa cauuuaauaa aaguagaaaa uuggauauuu      480

agaaauccag gauuugcacu uguagccgua gccauagcuu ggcuacuugg uagcucaaca      540

agucaaaaag uaauauauuu aguaaugaua uuacuaauag ccccagcaua caguauaagg      600

ugcauaggug uaucaaauag agauuuugua gaagguaugu caggcggaac auggguagau      660

guaguacuug aacacggagg augcguaaca guaauggcgc aagacaaacc cacaguagac      720

auugaacuag uaacaaccac aguaagcaau auggcugaag uaagaucuua uuguuacgaa      780

gcaaguauaa gcgauauggc aagugauuca agauguccua cacaaggcga agcauauuug      840

gauaagcaaa gugacacuca auauguuugu aaaagaacuu uagucgacag aggaugggga      900

aauggaugcg gacuuuuugg aaaaggaagc cuaguaacau gcgccaaauu cacauguagu      960

aaaaaaauga caggaaaaag uauacaaccu gaaaaccuag aguacagaau aaugcuuagu     1020

guacacggaa gccaacacuc cggaaugauu guaaaugaua uuggucauga aacagacgaa     1080

aauagagcaa aaguagaagu uaccccaaac ucaccuagag cagaggcaac acuaggugga     1140

uuuggcucau uaggauuaga uugugaaccc agaacagggc uagacuuuag ugacuuauau     1200

uaucuaacga ugaacaauaa acauugguua guccauaaag agugguucca ugauauaccc     1260

cuaccauggc augcgggagc agauacuggu acaccacauu ggaacaacaa agaagcauua     1320

guagaauuua aagacgcuca ugcuaaaaga caaacuguag uaguauuagg aucacaagaa     1380

ggugcagugc acacagcauu agcaggugcu cuagaagcag aaauggaugg agcaaaagga     1440

agauuauucu cagggcaucu aaaaugccga uuaaaaaugg auaaauuaag auuaaaaggg     1500

guaaguuauu cauuauguac agcagcauuc acuuuuacua aaguaccagc agaaacauua     1560

cacgguacag ucacaguaga aguacaauau gcaggaacag auggaccaug caaaguacca     1620

gcacagaugg caguagauau gcaaacauua acaccaguug gacguuuaau aacagcgaau     1680

ccaguaauaa cagaaucaac agaaaauagu aaaaugaugc uagaguugga uccgccauuu     1740

ggggauucuu acauaguuau aggagugggu gacaaaaaaa uaacucacca uuggcacaga     1800

agcgguucaa caauaggaaa agcauuugaa gcaacaguaa gaggagccaa acgaauggca     1860

guacucggag auacagcaug ggauuuugga ucagucggag guguauuuaa cucauuagga     1920

aaagguauac aucagauauu cggggccgcu uuuaaaucau uauuuggcgg aaugucaugg     1980

uucagucaaa uauuaauugg cacuuuauug guuuggcuag gacuuaauac aaaaaauggg     2040

aguauaagcc uuaccuguuu agcguuagga ggaguuauga uauuuuuguc aaccgcagua     2100

agugcggaug uaggaugcag uguagacuuc ucaaaa                               2136


<210>  2627
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long CDS opt

<400>  2627
aggauuauaa acgcaaggaa agagagaaaa agacguggag ccgauacaag caucggaaua       60

auaggccuau uacuaacaac agccauggca gcugagauua caagaagagg aagcgcauau      120

uacauguauu uagauagaag ugaugcugga aaagcaauau cauuugcaac aaauuuagga      180

guaaauaaau gucaugucca aauaauggac uuagggcaca ugugcgaugc aacaaugagc      240

uaugaauguc caauguuaga ugaaggaguc gaaccagaug acguagauug uugguguaau      300

acaacaucaa cauggguagu auacgguacu ugccaucaua agaaaggaga agcgagaaga      360

ucaagacgag ccgugacucu cccgagucac aguacacgua aauuacaaac ucgaucacaa      420

acaugguuag aaucucgaga auauacaaaa cauuuaauca aaguagaaaa cuggauauuc      480

agaaauccag gauucgcacu agccgcagua gcaauagcau gguuauuagg gaguaguacu      540

ucucaaaaag uaaucuaucu cguaaugauc cuauuaauug caccagcaua uagcauacgu      600

ugcauaggag uaagcaacag ggauuuugug gaagguauga guggaggaac auggguagau      660

guaguauuag aacacggagg uugcguaacu guaauggccc aagacaaacc aacaguagau      720

auagaacucg uaacaacaac aguaucaaau auggcagaag uaagaaguua uuguuaugaa      780

gccaguauuu cagauauggc aucagacucc agaugcccga cacaagggga agccuauuua      840

gacaagcaaa gugauacaca auauguaugu aaacgaacau uaguugauag gggauggggu      900

aauggaugug gauuauucgg aaaaggauca uuaguaacau gugcaaaauu cacuuguucc      960

aagaaaauga ccggaaaauc aauacaaccc gaaaacuuag aauacagaau aaugcuaucu     1020

guacauggau cacaacacag uggaaugaua guaaaugacg aaaauagggc aaaaguagaa     1080

gucacaccaa acagcccucg agcggaagca acauuaggag gauuuggaag ucuaggauua     1140

gacugugaac caaggacugg ucuagauuuu ucggaccuau auuauuuaac caugaauaac     1200

aaacauuggu uaguacauaa agaaugguuu caugauauuc cauuaccgug gcaugcuggu     1260

gcagauacag gaacaccgca uuggaacaau aaagaagcau uaguagaauu uaaagaugca     1320

caugcaaaaa gacaaacagu aguaguuuug ggcagccaag aaggugcagu ucauacugca     1380

cuggcuggag cucucgaagc agaaauggau ggagccaaag gaaggcuauu uucaggacac     1440

uugaaaugua ggcuaaaaau ggauaaacua agauuaaaag gcguaucaua uucucuaugu     1500

acagcggccu uuaccuuuac aaaaguucca gcagaaacuc uacauggcac aguuaccgua     1560

gaaguacaau augcaggcac agauggacca ugcaaaguac cagcacaaau ggcaguagac     1620

augcaaacau uaacacccgu agggagauua auaacagcaa auccaguuau aacugaauca     1680

acugaaaaca gcaaaaugau guuggaacua gauccaccuu ucggcgauag uuacauagua     1740

auagguguag gagauaagaa aauaacacau cacuggcauc guaguggaag cacaauagga     1800

aaagcauuug aagcaacagu aagaggggcu aaaagaaugg caguacuagg agauacagcu     1860

ugggacuucg gaucaguagg ggguguauuu aauagcuuag gaaaaggcau acaccaaaua     1920

uuuggagcag cauuuaaaag uuuauuuggg ggaaugaguu gguucucaca aauauuaaua     1980

ggaacuuuau ugguuugguu aggucucaac acuaaaaacg guaguauauc auuaacaugu     2040

cuagcacuag gaggaguaau gaucuuuuua ucaacagcag uaucagcaga cguagguugu     2100

aguguagauu uuucaaaa                                                   2118


<210>  2628
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long CDS opt

<400>  2628
cgaauaauua augcaagaaa agaaagaaaa cguaggggag cagacacauc aauuggcauu       60

gucggauuau uauuaacaac ugcuauggca gcagaaauca caagaagagg uagugcuuau      120

uauauguauu uagauaggag ugaugcagga aaagcaauau cauuugcaac cacacuaggc      180

guaaauaaau gucaugugca gauaauggau uuaggacaua ugugcgaugc uacuaugagu      240

uaugaauguc caauguuaga ugaaggagua gaacccgaug auguagacug uugguguaac      300

acaacgagua cauggguggu auauggaaca ugucaucaua agaaaggaga agcaagaagg      360

aguagaaggg ccguuacacu accuagucau aguacaagaa aauuacaaac aagaagccaa      420

acaugguugg aaagccgcga auacacaaaa cauuuaauaa aaguagaaaa uuggauuuuc      480

agaaauccgg gcuuugcccu aguagcagua gcaauugcau gguuacuagg aaguuccaca      540

agucaaaaag uaauuuauuu aguaaugaua uuacuaauag cgccagcaua cucuauaagg      600

uguauuggag uaaguaauag agauuuugua gaaggcauga guggaggaac uuggguagau      660

guaguacuag aacauggagg augugucaca gugauggccc aagacaaacc cacaguagau      720

auagagcuag uaacaacuac cguaaguaac auggcggaag uaagaucaua cuguuaugaa      780

gcaaguauau cagacauggc uuccgauagu agauguccga cacaaggaga agcguaccug      840

gauaaacaaa gcgacacaca auauguaugu aaaagaaccu uaguagauag aggaugggga      900

aaugguugug guuuauuugg aaaggguagc uuaguaacau gugcaaaauu uacauguuca      960

aagaaaauga cgggaaaauc aauacaaccu gaaaacuugg aauauagaau aaugcuaagu     1020

guacauggau cacaacauag uggaaugaua guaaaugaua caggacauga aacggaugaa     1080

aaucgagcaa aaguggaagu aacgccaaac ucaccaagag cggaagcgac auuaggaggu     1140

uucggaucau uaggguugga cugugaacca cgaacaggac uagauuucag ugauuuauac     1200

uauuuaacua ugaauaacaa acauugguua guucauaaag aaugguuuca ugauauacca     1260

uuaccauggc acgcaggcgc agauacggga acaccacauu ggaacaauaa agaagccuua     1320

gucgaauuua aagaugcaca cgcgaaacgc caaacuguag uaguuuuagg aucacaagaa     1380

ggagcaguac auacagcauu agcuggugca uuggaagcgg aaauggaugg agcaaaagga     1440

agauuauuua gcggucauuu aaaaugucga cuaaaaaugg auaaauuaag auuaaaggga     1500

guaucauauu cacuauguac agcagcauuu acuuuuacca aaguuccagc ugaaacauua     1560

cauggaacgg uaacuguaga aguacaauau gcaggaacug auggaccuug uaaaguaccu     1620

gcucagaugg caguugauau gcaaacauua acaccuguag gacgauugau aaccgcaaau     1680

ccagugauaa cagaaucuac agaaaauagu aaaaugaugu uggaauuaga uccaccuuuu     1740

ggugacaguu auauuguaau aggaguaggu gauaagaaaa uuacacacca cuggcacagg     1800

agugguagua cuauaggaaa agccuuugaa gcuacaguaa gaggagcaaa aagaauggcu     1860

guguuaggag auacagcuug ggacuuuggu ucaguuggcg gaguuuuuaa uucguuaggg     1920

aaaggcauuc aucaaauauu cggugcagca uucaaaagcc uauucggagg aaugagcugg     1980

uucagucaaa uacuuauagg aacacuauua guguggcuug guuuaaauac gaaaaaugga     2040

ucaauaaguu uaacauguuu agcauuaggu gguguaauga uauucuuaag uacagcagua     2100

agcgcagaug uaggauguuc uguagacuuc ucaaaa                               2136


<210>  2629
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long CDS opt

<400>  2629
agaauaauaa augcaagaaa agagcgaaaa agaagaggag cagacacauc uauugggaua       60

guaggacuau uauuaacaac agcgauggcg gcugaaauua caagaagagg aagugcuuac      120

uauauguacu uagaccgaag ugaugcuggc aaagcaauau cguucgcuac aacacuuggg      180

guaaacaaau gccacguuca aauaauggac uuaggacaua ugugugaugc uacgaugagc      240

uacgaauguc caauguuaga ugaaggugua gaaccagaug acguagacug uugguguaac      300

acaacaagua cauggguagu uuauggaaca ugucaacaca agaaagggga agcaagacgu      360

agcagacgag cagugacacu accuagucau aguacuagaa aacuacaaac aagaucacag      420

accugguuag aaaguagaga auauacaaaa caucuaauaa aaguagaaaa uuggauauuu      480

agaaaucccg gauuugcccu aguugcagua gcaauagcau gguuauuagg aucaagcaca      540

agucaaaaag uaauauauuu aguaaugaua uuacuuauag caccagcuua cagcauuaga      600

uguauaggug uaucaaauag agacuuugug gaggguauga gcggaggaac auggguugau      660

guaguauuag aacauggagg cuguguuacu guaauggcuc aagacaaacc cacaguagau      720

auagaauuag uaacgacaac aguaucgaau auggcagaag ucagaucuua uuguuacgaa      780

gcgagcauau cagacauggc aagugauagu agauguccca cucaagguga agcauauuua      840

gacaaacaau cagauacaca auauguaugu aaaaggacau uaguagaccg ggguugggga      900

aauggaugcg gacuauuugg uaaaggcagc uuaguuacau gugccaaauu uacauguucu      960

aagaaaauga caggaaaaag uauacaacca gaaaauuuag aauauagaau uauguuaagu     1020

guacauggaa gccaacauuc aggaaugauc guaaaugaca uuggacauga gacugaugaa     1080

aauagagcaa aaguagaggu aaccccaaau ucuccuaggg cagaagcaac acucggagga     1140

uucggaaguu uaggacuaga uugugaacca agaacaggac uagauuuuuc agauuuauac     1200

uaucuaacaa ugaauaauaa acauuggcua gugcauaaag aaugguuuca cgacauuccg     1260

cuaccauggc augcaggagc agauaccgga acaccacauu ggaauaacaa agaagcucug     1320

guagaauuua aagaugcaca ugcaaaaagg caaacgguag uaguucuugg aucacaagaa     1380

ggagcaguac acacggcauu agcaggagca uuagaggcug aaauggaugg ugcuaaaggu     1440

cggcuauuuu caggucauuu aaaaugcagg uuaaaaaugg auaaauugag auuaaaagga     1500

guaagcuaua gucuauguac agcuguuugc acagcugcaa aaguaccagc agaaacauua     1560

cauggaacug uaacaguaga aguacaauac gcaggaacag auggaccuug uaaaguacca     1620

gcacaaaugg cuguagauau gcaaacacuu accccaguag gacgauuaau uacagcaaac     1680

cccguaauaa cagagucaac ggaaaauagu aaaaugaugu uagaauuaga cccucccuuu     1740

ggugauucau acauaguaau aggaguagga gauaagaaaa uaacacacca uuggcacaga     1800

ucaggcucaa caaucggaaa agccuucgaa gcuacaguac gaggagcaaa aagaauggca     1860

guauuaggag auacugcaug ggauuuuggc aguguaggag gaguauuuaa uaguuuaggu     1920

aaagguauuc aucaaauuuu uggagcagcu uucaaaucau uauucggagg aaugaguugg     1980

uuuucacaaa uacuuauagg aacacuauua guaugguuag gacuuaauac gaaaaacggg     2040

ucaaucagcu uaacauguuu agcacuaggu ggcguaauga uauuucuuuc aacagccguu     2100

agugcagaug uaggcuguuc cguugauuuc agcaaa                               2136


<210>  2630
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long CDS opt

<400>  2630
agaauaauaa acgcuagaaa agaacggaag agaagaggag cugaugcguc aauaggaaua       60

guaaguuuau uauuaacaac aguaauggcu gcagaaauua caaggagagg aucagcuuau      120

uauauguauu uagacagaag ugaugcggga aaagcaauaa guuuuguaac aacauuagga      180

guaaauaaau gucaugucca aauaauggac uuggggcaua ugugcgaugc aacaaugucu      240

uaugagugcc caauguugga ugaaggaguu gaaccagaug auguagauug uugguguaau      300

acaacaucaa cuuggguagu auacggaaca ugccaccaca agaaagggga agcuagaaga      360

ucaagaaggg caguaacauu gccaagucau aguacgagaa aacuacaaac acguagccag      420

acauggcuug aaucaagaga auauacaaaa cacuugauaa aaguagagaa uuggauuuuc      480

cgaaauccag guuucgccuu aguugcagua gcaauugcau gguuacuagg aagcagcacu      540

ucucaaaaag uaauauauuu aguaaugaua uuacuaauag caccagcaua uucaauaaga      600

uguauaggag ugaguaacag agauuucgua gaagguauga guggaggaac auggguagac      660

guaguacucg aacacggugg augcguaaca guaauggcuc aagacaaacc aacaguagau      720

auugaacucg uaacaacaac aguaucaaau auggccgaag uuaggucuua uugcuaugag      780

gcaucaauau cagacauggc aucagauagc agauguccua cacaaggaga agcauauuua      840

gauaaacaau cagacacaca auacguaugc aaaagaacau uaguagauag aggcugggga      900

aacggcugug gacuguuugg gaaaggaucu cucguaacau gcgcuaaauu caccuguagu      960

aagaaaauga caggaaaaag uauacaacca gaaaaccuag aauauagaau uauguuaagc     1020

gugcauggaa gccaacacag cgggaugaua guuaaugaug aaaauagggc aaaaguagag     1080

gucacgccaa acagcccaag agcagaagca acauuaggag guuucggaag ccucggauua     1140

gauugugaac caaggacugg auuagauuuc ucagauuuau auuaucuuac aaugaauaac     1200

aaacacuggc uaguacauaa agaaugguuu cacgauauuc cauuaccaug gcauucagga     1260

gcagacacug aaacaccaca cuggaauaau aaagaagcgu ugguagaauu uaaagaugca     1320

cacgcaaaaa gacaaaccgu aguagucuua gguucgcaag aaggagcagu acauacagca     1380

cuagcaggcg cgcuagaagc ugaaauggau ggugcaaaag gaagacuguc aucaggucau     1440

cuaaaaugua gauuaaaaau ggauaaauua agacuaaaag guguaucaua uuccuuaugu     1500

accgcagcgu uuacuuucac aaaaguucca gccgaaacau uacauggaac aguaacugua     1560

gaaguacaau augcagggcg agacggaccu uguaaaguuc cagcacaaau ggcaguugau     1620

augcaaacau uaacaccagu ugggagauua auaaccgcaa acccaguaau aacugaauca     1680

accgaaaauu caaagaugau guuagaauua gaucccccau uuggagacuc cuauauagug     1740

auaggaguug gagauaagaa gauaacccau cauuggcaua gaucaggaag cauuauagga     1800

aaagcauuug aagcaacagu aagaggcgca aaaagaaugg caguacuagg cgauacggca     1860

ugggacuuug gaucaguggg aggaguuuuc aacagucuag gaaaagguau ucaccaaaua     1920

uucggagccg cauucaaaag ccuauucgga gguaugucau gguuuucaca gauucuaaua     1980

ggaacgcuuc uaguaugguu gggauuaaau acuaaaaaug gaaguauauc acuaacaugu     2040

cuugcuuuag gaggaguaau gauauucuug ucaacagcag uuucugcaga cguuggaugc     2100

aguguggauu uuaguaaa                                                   2118


<210>  2631
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long CDS opt

<400>  2631
agaauaauaa augcaagaaa agaaagaaaa agaagaggag cugauacauc uauuggaaua       60

guaggcuugu uauuaacaac agcuauggca gcagaaauaa caagaagagg aagcgcuuac      120

uauauguauu uagacaggag ugaugccgga aaagcaauau cauuugcaac aacauuaggg      180

gucaauaaau gucaugucca aauaauggac uuaggacaua ugugcgacgc aacaaugagu      240

uaugaguguc caauguugga cgaaggagua gaaccagaug auguagauug uugguguaau      300

acgacuucca cauggguagu uuacggaacu ugucaucaua agaaaggaga agcuagaaga      360

agcaggagag caguaacacu accaucacau aguacuagaa aacuucaaac aagaagccaa      420

acauggcuag agucaagaga auauacaaaa cacuugauaa aaguagaaaa uuggauauuu      480

agaaauccag gcuuugcauu aguggcagua gcuauagccu ggcuuuuagg aagcaguaca      540

agucaaaaag uaauauauuu aguaaugaua uuauuaauag caccagcaua uucaaucaga      600

ugcaucggag ugaguaauag agacuucgua gaagguauga gcggaggaac auggguagau      660

gugguacuag aacacggagg cuguguaaca guaauggcac aagauaagcc gaccguagau      720

auugaauuag uaaccaccac cgugaguaau auggcagaag uuagaucaua uugcuaugaa      780

gcaaguauca gugauauggc aagcgauagu agauguccga cacaaggaga agcauaccua      840

gauaaacaaa gcgacacaca auacguaugu aaaagaacac ucguagauag aggaugggga      900

aacggaugug gauuauuugg gaaaggaucc cuaguaacau gugccaaauu uacuuguucg      960

aagaaaauga cuggaaaaag uauacaaccu gaaaacuuag aauauagaau aaugcuauca     1020

guacauggua gccaacauag cggaaugaua guaaaugaug aaaacagggc aaaaguagaa     1080

guaacgccua auagcccaag agcagaagcu acacuaggug gauuuggaag uuuaggacua     1140

gauugcgaac cacgaacggg guuagauuuu agugacuuau auuauuuaac uaugaacaau     1200

aaacauuggu uaguacacaa agaaugguuu cacgacauac cauuacccug gcaugcagga     1260

gcggauacag gaacaccaca uuggaauaac aaagaagcuc uaguggaauu uaaagaugca     1320

caugcaaaaa ggcaaacagu uguaguacug ggaucacaag aaggagcggu ucauacagcc     1380

cuagcaggag cacuagaagc cgaaauggau ggagcgaaag gaagauuauu cucaggacau     1440

uuaaaauguc gauuaaaaau ggacaaacua cgauuaaaag gaguuaguua cucauuaugu     1500

acagcagccu ucacguuuac aaaaguacca gcagaaacac uacacggaac aguaacugua     1560

gaaguacaau acgcuggaac agauggaccu uguaaaguac cugcccaaau ggcgguagau     1620

augcaaacau uaacaccugu ggguagguua auaacagcaa auccaguaau aacugaauca     1680

acagaaaaca guaaaaugau guuagaauua gauccacccu uuggugauag cuauauaguc     1740

auaggaguag gugacaagaa aaucacacau cauuggcacc gaaguggcuc aacgauaggu     1800

aaagcauucg aagcaacagu ucgaggagcu aaaagaaugg caguacuagg agauacagcc     1860

ugggauuuug ggaguguagg uggaguauuc aauagccuag ggaaaggaau acaccaaaua     1920

uucggggcag cauucaaaag ucuauuuggu gguaugaguu gguuuucaca aauauuaaua     1980

ggcacuuuau uaguauggcu aggguuaaau acuaaaaacg gaagcauuag cuuaacaugc     2040

cuagcgcugg gagguguaau gauauuucua aguacagcag uaagugcaga uguaggaugu     2100

agcguugauu uuucaaag                                                   2118


<210>  2632
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long CDS opt

<400>  2632
agaauuauaa augcaagaaa agaaagaaaa agaaggggag cugacacuuc aauaggaaua       60

auaggauuac uacuuaccac agcaauggca gcagaaauaa cacgaagagg aucugcauau      120

uacauguauc ucgauagaag cgacgcaggu aaagccauaa guuuugcaac cacauuaggu      180

guaaauaagu gucauguaca aaucauggac uuaggacaua ugugugacgc aaccaugagc      240

uaugaaugcc cuaugcuaga cgaaggagua gaacccgaug acgucgacug uuggugcaac      300

acuacaagca caugggucgu uuaugguacc ugucaucaua agaaaggaga aacucgaaga      360

ucaagaagaa guguaucucu uagauaucau uacacaagaa aacuacagac aagaucucaa      420

acauggcuag aaucaagaga auauaagaaa caucuaauaa ugguugaaaa uuggauauuc      480

aggaacccag gauuugcaau aguuaguguu gcaauaacau ggcuuauggg aaguuuaaca      540

agccaaaaag uuauauacuu aguaaugaua guucuaauug uaccagcuua uucaauaagc      600

ugcauaggag ugaguaacag agauuuaguc gaaggaaugu cagguggaac auggguagau      660

guaguauuag aacauggggg augcguuaca gaaauggccc aagauaaacc gacaguagau      720

aucgaauuag uaacaaugac cguaucaaac auggccgaag uaagaucaua uuguuaugaa      780

gcaucacuau cagauauggc aagugcaagc agaugcccaa cucaagggga accaucucua      840

gauaaacaau cagacacaca aucaguaugc aaaagaacau ugggugauag aggauggggc      900

aacggcugug gcauuuuugg aaaaggaagu cuaguuaccu guaguaaauu cacgugcugu      960

aagaaaaugc caggaaaaag uauucaacca gaaaaccuug aauacagaau uauguuacca     1020

guacauggau cgcaacauag uggcaugaua gugaaugaua uaggucacga aacagacgaa     1080

aauagagcaa aaguugaagu gacaccaaac aguccaagag cagaagcaac auuaggagga     1140

uuuggaucau uaggauuaga uugugaacca cgaaccgggc uagauuucag cgaucuuuau     1200

uacuuaacga ugaauaauaa acacuggcua guacauaaag aaugguucca ugacauacca     1260

uuaccauggc acgcaggagc agauacugga acuccacauu ggaauaauaa agaagcacuu     1320

guugaguuca aagaugcaca ugcaaaaagg caaacaguag uaguacuagg aagccaagaa     1380

ggagcuguuc acacagcguu agcaggcgca cuugaagcug aaauggaugg agcaaaagga     1440

agauuguuuu caggacauuu aaagugcaga cuuaaaaugg acaaacuaag acuaaaaggc     1500

gucucuuacu cauuauguac agcagcauuu acauucacua aagugccagc agaaacacua     1560

cauggaacug ucacaguaga aguccaaucu gcagguacag auggcccaug uaaaguaccc     1620

gcccaaaugg caguugacau gcaaacgcug acaccaguag guagacuaau aacagcaaac     1680

ccuguaauca cagaaucaac agaaaacucc aaaaugaugu uagaacuaga uccaccuuuc     1740

ggagauaguu auauuguaau uggaguagga gauaagaaaa uaacacauca uuggcauaga     1800

agcggaagua cuauaggaaa agccuucgaa gcuacaguaa gaggcgcuaa aaggauggcu     1860

gucuuaggug auacagcuug ggauuuugga ucaguagggg guguauucaa uagucuagga     1920

aaaggaauac aucaaauuuu uggagccgcg uucaaaaguu uauucggagg uaugucgugg     1980

uuuucacaaa ucuuaauagg aacauuacug guauggcuag gacuaaauac uaaaaauggc     2040

aguauuagcu uaacauguuu agcuuuagga ggaguaauga uauuucuuag cacagcaguu     2100

agugcggaug ucggauguuc uguagacuuu ucuaaa                               2136


<210>  2633
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long CDS opt

<400>  2633
agaauuauua acgcucguaa agaaagaaaa agacguggug cagacacaag cauagggaua       60

auagguuugc uauuaacaac ugcaauggcu gcugaaauaa caagacgagg aucagcauau      120

uauauguacc uggauagguc ugaugcagga aaagcaauau cauucgcaac uacaauggga      180

guaaauaaau gucauguaca aauaauggau uuaggacaca ugugugaugc uacaaugagu      240

uaugaauguc cuauguuaga cgaaggagua gaaccugaug auguugacug uugguguaau      300

acuacuagca cauggguugu auaugggacc ugccaucaua agaaaggaga agcaaggcca      360

ucaagacgag cagucacauu accgagucau aguacucgaa aauuacaaac ucgaagucaa      420

acgugguuag aaucacguga auacacaaaa cacuuaauaa aaguagaaaa uuggauacuu      480

cgaaacccug gguuuacggu agcagccuua gcaauggcau ggcuucuagg aaauaguaca      540

agucaaaggg uaauauaccu aguaaugauu uuauuaauag cacccgcaua uucaauaaga      600

ugcauaggag uaagcaauag agauuuugua gaagguaugu caggaggaac auggguagau      660

guuguacuag aacacggcgg augcguaaca guuauggcuc aagacaaacc aacaguagau      720

auagaauuag uaacuacaac aguaucaaau auggcugaag uaaggaguua uuguuaugag      780

gcaaguaucu cagauauggc aagugacagu aggugcccaa cccaaggcga ggcuuaucua      840

gauaaacaaa gugauacuca auacguaugu aaaaggacgc uaguagauag aggaugggga      900

aacgguugug gacuauuugg aaaaggaagc cuaguaacau gugccaaauu uacauguuca      960

aagaaaauga caggaaaaag uauacaaccc gaaaaucuag aauauagaau uaugcuaagu     1020

guacauggau cucaacacuc aggaaugaua guaaaugaca uaggacauga aacagaugaa     1080

aauagagcaa aaguggaagu uacaccaaau aguccaagag cagaagcuac acugggugga     1140

uucgggagcu uaggguuaga uugugaacca aggacaggau uagauuucuc agaucuauac     1200

uaucuaacaa ugaacaacaa acauugguua guucauaaag aaugguuuca cgacauacca     1260

cuaccauggc acgcaggagc agauacagga acaccacauu ggaacaauaa agaagcauua     1320

guagaguuca aagacgcaca ugcuaaaaga cagacaguag uuguucuagg aagccaagaa     1380

ggagcuguac auacagcacu ugcaggagca cuugaagcag aaauggaugg ugcaaaaggg     1440

agacuuuuca gcggacacuu aaaaugccga auaaaaaugg auaaauuaag auuaaaaggu     1500

gucucguauu cuuuauguac agcagcauuu acauuuagua aaguaccagc agaaacauua     1560

caugguacgg uaacagucga aguacaauau gcaggaagag auggaccaug uaaaguacca     1620

gcacagaugg caguagauau gcaaacauua acaccuguag ggagauuaau aacggccaau     1680

ccuauaauaa cagaaaguac agaaaauuca cgcaugaugc uugaacucga uccaccauuc     1740

ggagauaguu auauagugau aggaguaggu gauaagaaaa uaacccauca uuggcauaga     1800

aguggaagua cuauagguaa agcauucgaa gcaacaguga gaggagcuaa acgaauggca     1860

guauuaggcg auacagcuug ggauuuuggu aguguaggug gaguauucaa uagucuagga     1920

aaaggaguac accaaguauu cggagcggca uuuaagagcu uguucggagg aaugagcugg     1980

uucucacaaa uacuuauagg uacauuauua guauggcuag gauuaaauac uaaaaacgga     2040

aguauuucau uagcaugucu ugcucuaggu ggugcaauga uuuuucuaag cacugccguu     2100

ucugcagacg uugguuguag cguagauuuc                                      2130


<210>  2634
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short CDS opt

<400>  2634
acuagcguag gaauaguagg auuauuauua acgacagcaa uggcugcaga aguaacucga       60

cgcggaucag cauauuauau guaccucgac agaaaugacg caggagaagc aauuaguuuc      120

ccuacaacau uagggaugaa uaaauguuau auccaaauca uggauuuagg acacaugugu      180

gaugcaacaa ugagcuacga auguccaaug uuagacgaag guguagaacc agacgacgua      240

gacuguuggu gcaauacaac aucaacaugg guaguauaug guacaugcca ucauaagaaa      300

ggcgaagcua gaagaaguag acgagcagua acacuaccau cacauagcac aagaaaauua      360

caaacaagau cucaaaccug gcuagaauca agagaauaua cuaaacauuu gauaagagua      420

gaaaauugga uauuuagaaa uccaggauuc gcuuuagcag cagcugccau ugccuggcuu      480

uuaggaucau caacaagcca aaaaguaaua uauuuaguaa ugauauugcu uauagcgcca      540

gcauacagca uaagauguau aggaguuuca aauagagauu uugucgaagg aaugagcgga      600

ggaacauggg uagauauagu acuugaacau ggaggaugcg ugacaguuau ggcccaagac      660

aaaccuaccg uagacauaga auuaguuaca acuacuguau cuaauauggc cgaaguaaga      720

aguuauugcu acgaggcuuc aauuagugac auggcaucag auucuagaug cccaacucaa      780

ggugaagcau accuagacaa acaaagugac acgcaauacg ucuguaaaag aacauuagua      840

gacagaggau ggggcaaugg augcggauua uucggaaaag gcagcuuagu aacuugugca      900

aaauucgcau guagcaagaa aaugacagga aaaucaauac aaccagaaaa cuuagaauac      960

agaaucaugu ugucaguuca uggcagccaa cauucaggaa ugauaguaaa cgauacugga     1020

caugaaaccg augaaaauag agcaaaagua gaaauaacuc caaauagucc uagagccgaa     1080

gccacauuag gugguuuugg aucauuagga cuagauugug aaccacgaac aggacuagau     1140

uuuagugauu uauauuaucu aacgaugaau aauaaacauu gguuaguaca uaaagaaugg     1200

uuucaugaua uaccacuucc auggcaugca ggagccgaua cuggaacccc acacuggaau     1260

aauaaagaag cauuaguuga guucaaagau gcccacgcaa agagacaaac aguaguagua     1320

uuaggaucac aagaaggggc aguacauacc gccuuagcug gagccuuaga agcagaaaug     1380

gacggagcua aaggaagacu gaguagcgga cacuugaaau gucgacugaa aauggauaag     1440

uuaagguuaa aaggaguaag uuauaguuua uguacagcug cauuuacauu uaccaaaaua     1500

ccagccgaaa cccugcaugg gacgguuaca guagaaguac aauaugcagg aacggaugga     1560

ccauguaaag uaccagcgca gauggcaguu gauaugcaaa cacuaacacc aguaggaaga     1620

cuuauaaccg caaauccggu gauaacagaa aguacagaaa acuccaaaau gauguuagaa     1680

uuagauccac cauuuggaga cucauauaua gugauaggag ugggagaaaa gaaaauaaca     1740

caccauuggc auagaagugg aaguacaaua ggaaaagcgu ucgaagcaac cguaagagga     1800

gcaaaaagaa uggcaguucu aggagauacu gcaugggauu ucgguagcgu aggaggagca     1860

uuaaauucac uaggaaaagg aauccaucaa auauuuggag cagcauucaa aaguuuauuc     1920

ggaggaauga guugguuuuc ccaaauacua auaggaacac uauuaaugug guuaggauug     1980

aauacgaaaa auggaucuau aucgcugaug ugucuagcau uaggaggagu cuuaauuuuc     2040

uuaucaacag caguaucagc g                                               2061


<210>  2635
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short CDS opt

<400>  2635
acaucaguug gaauagucgg acuucuuuug acaacagcaa uggcugcuga aguuacgcga       60

agaggaagug ccuauuacau guauuuggac agaaaugaug caggagaggc uauuucauuc      120

ccaacaacau uagggaugaa caaauguuau auacaaauaa uggaucuugg ucauacaugc      180

gacgcgacga ugucauacga auguccaaug cuugaugaag gaguagaacc ugacgaugua      240

gacuguuggu gcaacacaac aucaacuugg guaguauaug gaacuuguca ucacaaaaaa      300

ggggaggcaa ggagaucacg uagagcaguu acacuaccuu cacauaguac aagaaaacua      360

caaacaagau cacaaacaug gcucgaauca cgagaauaua caaaacauuu aauaagggua      420

gaaaauugga uuuucagaaa cccaggauuu gcccuugcag cagcagcaau ugcauggcuu      480

uuaggaagua gcacaaguca gaaagugaua uacuuaguga ugauuuuacu uauagcucca      540

gcauauucua uaagaugcau aggaguaagu aauagagacu ucguagaagg aaugaguggg      600

gguacuuggg uagauguugu acuugaacac ggcggaugcg uaacaguaau ggcccaagau      660

aaaccaacug uagauauaga auuaguaacg acaacaguuu caaauauggc agaaguaaga      720

ucauauugcu acgaagcauc cauaucagac auggcuucug auucuagaug uccaacucaa      780

ggugaagccu auuuagauaa acaaucagau acucaauaug uauguaaaag aaccuuagua      840

gacagagguu ggggaaaugg augcggauua uuuggaaaag gaagucuagu aacgugugca      900

aaauuugcau gcaguaaaaa aaugacugga aaaaguauuc aaccagaaaa ccuugaauau      960

agaauaaugu ugaguguaca ugguagccaa cauucaggaa ugauaguaaa ugauacaggc     1020

cacgaaacag acgaaaauag agcaaaaguu gaaauaacac caaauagucc cagagccgaa     1080

gcaaccuuag gaggauucgg aucucuagga uuagacugcg aaccuagaac aggacuagac     1140

uuuucagauc uuuacuauuu aacaaugaau aauaaacauu ggcuagugca uaaggaaugg     1200

uuucacgaua uaccauuacc auggcaugca ggagcugaua cagguacacc acauuggaau     1260

aauaaagaag cacuuguaga auucaaagac gcacacgcua aacgacaaac aguaguagua     1320

uuagguaguc aagaaggagc aguucauaca gcauuagcug gggcauuaga agcagaaaug     1380

gauggagcaa agggaaggcu aucaucagga cauuuaaaau gcagauuaaa aauggauaaa     1440

uugagacuca aagguguauc auauucucua uguacagcag cuuucacuuu uacaaaaaua     1500

ccagcagaaa cucuucaugg gacuguuaca guugaaguac aauaugcagg aacagauggu     1560

ccaugcaaag uaccagcaca aauggcagua gauaugcaaa cauuaacacc aguaggaaga     1620

cugauaacag caaacccagu aauaacagaa agcacagaaa auucaaaaau gauguuagaa     1680

uuagauccuc cauucggaga uucauauaua gucauaggag uaggagaaaa aaaaauaaca     1740

caucauuggc auagaucagg aucaacuaua ggcaaagcau uugaagcaac aguaagaggc     1800

gcaaaaagaa uggccguacu aggagacaca gcuugggacu ucggaagcgu aggaggagca     1860

cuaaauaguu uaggaaaagg aauucaccaa auauuuggag cagcauuuaa aaguuuauuc     1920

ggaggaauga gcugguucuc acaaauauua auagguacuu uacuaaugug guuaggacua     1980

aaugccaaaa acggaucaau aagcuuaaug ugcuuagcuc uuggaggugu gcuaauauuc     2040

cuuucaacag caguaagugc u                                               2061


<210>  2636
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short CDS opt

<400>  2636
acaucaauug gaauaauugg ucuuuuacuu acuacagcaa uggcagcaga aauaacaaga       60

agaggaucag cauauuauau guauuuagac cguagcgaug ccggaaaagc aauaucauuc      120

gcuacaacau uaggaguuaa uaaaugucau guucaaauaa uggacuuagg acauaugugu      180

gaugcaacua ugucuuauga auguccaaug cuagacgaag gaguagaacc agaugaugua      240

gauugcuggu guaauacuac uagcacaugg guuguauaug guacauguca ucauaagaaa      300

ggcgaagcua gaagaaguag acgagcagua acuuuaccau cucauucgac aagaaaauug      360

cagacgagau cacaaacaug guuagaaagu agagaauaua cuaaacauuu aauuaaagua      420

gaaaacugga uauuuagaaa uccggguuuu gcauuagugg cagucgcuau ugcaugguua      480

uuaggcucau caacgaguca aaaaguaaua uaucuaguaa ugauacuauu gauagcccca      540

gccuauucaa uaagaugcau aggaguaagu aauagagauu uuguagaagg uaugagcgga      600

ggcacuuggg uagaugucgu uuuagaacac ggaggaugcg uaacaguaau ggcucaagac      660

aaaccaaccg uagauauaga guuaguuaca acaacaguaa guaauauggc agaaguucga      720

ucuuacuguu augaagcauc aauaucagau auggcuagcg acucuagaug ccccacacag      780

ggagaagccu acuuagauaa acaaagugau acccaauacg uauguaaaag aacccuagua      840

gauagaggau ggggcaaugg gugcggacua uuugguaaag gaucucuagu gacaugcgca      900

aaauucacau gcucaaagaa aaugacagga aaaaguaucc aaccagaaaa uuuagaauau      960

agaauaaugu uaucuguaca uggcucacaa cauucaggua ugauaggaua ugaaacugau     1020

gaagacagag caaaaguaga agucacacca aacuccccaa gagcagaagc aacacuuggc     1080

ggguuuggaa guuuaggauu agacugugaa ccuagaacag gauuagacuu cagugauuua     1140

uauuaccuaa cgaugaacaa uaaacacugg uuaguacaua aagaaugguu ucacgacaua     1200

ccauuaccgu ggcaugcagg agcagauacu ggaacaccuc auuggaacaa uaaagaagca     1260

uuggucgagu ucaaagaugc ucaugcaaaa agacaaaccg uaguaguauu aggaucacaa     1320

gaaggagcug uacauacagc auuagcagga gcgcuagaag cagaaaugga uggagcaaaa     1380

ggaaggcuau ucucaggcca ucuaaaaugu aggcuuaaaa uggauaaacu acgacugaaa     1440

ggaguaaguu acucauuaug uacagcagcc uucaccuuca cgaaaguacc agccgaaacu     1500

cuacacggaa caguaacugu agaaguacaa uaugcaggua cagauggacc cuguaaaaua     1560

ccaguacaaa uggcaguaga uaugcaaaca uuaacaccag uaggaagauu aauuacugca     1620

aauccaguaa uaacagaaag uacagaaaau ucaaaaauga ugcuugaacu ugauccccca     1680

uuuggagauu cauauauagu aauaggagua ggagauaaga aaauaacaca ucauuggcau     1740

agaucaggau cgacaauugg aaaagcauuc gaagcaacag uaagaggagc gaaaagaaug     1800

gcuguauuag gagacacagc gugggauuuu ggaucagugg gaggugucuu uaauagcuua     1860

ggaaagggaa uacaccaaau auucggugcc gcguuuaaau cacucuuugg aggcauguca     1920

ugguucaguc aaauacuaau aggaacucug uuaguguggc uaggauuaaa uacgaaaaau     1980

ggaagcauua gcuuaacaug uuuagcauua ggaggaguga ugauauuccu aaguacagca     2040

guaucagca                                                             2049


<210>  2637
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short CDS opt

<400>  2637
acaucaguag gaauuguugg auuauuacua acaacagcua uggcagcuga ggucacaaga       60

agaggaucug cauauuauau guauuuagau aggaaugaug caggagaagc uauaaguuuc      120

ccgacaaccc uagguaugaa uaaauguuau auucaaauaa uggaucuagg acacaugugu      180

gaugcuacaa ugucauacga augcccuaug uuagaugaag gcguagaacc agacgaugua      240

gauugcuggu guaauacaac gagcacaugg guaguauaug gaacuugcca ucauaagaaa      300

ggagaagcua gaagaaguag gagagcugua acauuaccua gucauaguac aagaaaauua      360

caaacaagau cgcaaacaug guuggaauca agagaauaua cgaaacauuu aauaagagua      420

gaaaauugga uauuuagaaa uccaggguuc gcuuuagcag cggcugcuau ugcaugguua      480

uuagggagua gcacuucaca aaaaguaaua uauuuaguga ugauauuauu aauagcucca      540

gcauauucaa uaagaugcau aggaguguca aauagagauu ucguagaagg aaugucgggu      600

ggaacauggg uagauguagu uuuggaacau ggcgguugcg uaacaguaau ggcucaagac      660

aaaccuacag uagauauaga auuaguaaca acaacaguaa guaauauggc agaaguuaga      720

ucuuauugcu augaagcauc aauuucagau auggcaagug acucacgaug ccccacacaa      780

ggggaagcau accuagacaa acaaucagau acucaauacg uauguaaaag aacauuagua      840

gaccgggguu ggggaaacgg augcggucua uucggaaaag gaagucuagu aacaugugcu      900

aaauuugcau guaguaagaa aaugacaggg aaaaguauuc agccugaaaa cuuagaauau      960

agaauaaugc ugucuguaca uggaagucaa cauaguggua ugauaguaaa ugauacagga     1020

caugaaacag acgaaaauag ggcaaaagug gagaucacgc caaacagucc uagagcagaa     1080

gcuacacuag gagguuuugg aagucuaggg cuagauugug aacccagaac uggauuagau     1140

uuuucggauu uauacuacuu aacaaugaac aacaaacacu gguuggucca uaaagaaugg     1200

uuucaugaua uaccauuacc auggcaugcg ggcgcagaua caggaacacc acauuggaau     1260

aacaaagaag cauuagucga auuuaaagau gcccaugcaa aaagacaaac cgugguaguc     1320

uuaggaaguc aagaaggagc aguacauaca gcacuagcug gcgcgcuuga agcagaaaug     1380

gauggagcua aaggaaggcu aucgucagga cauuuaaaau guagguugaa aauggauaaa     1440

cuaaggcuaa aaggaguuag uuauagcuug uguacugcag cauucacguu uacaaaaaua     1500

ccagcagaaa cauuacacgg uacaguuaca guugaaguac aauaugcagg gaccgaugga     1560

ccauguaaag uacccgcaca aauggcugua gauaugcaga cacuaacacc cguaggaaga     1620

cuaauaacag caaauccugu cauaacagaa aguacagaaa acucaaaaau gaugcuugaa     1680

uuagauccac cauuuggaga uucuuacaua guaauaggag uaggagaaaa gaaaauaacu     1740

caucacuggc aucgaagugg aaguacaaua ggaaaagcau uugaagcaac cguuagagga     1800

gcaaaaagaa uggcaguauu aggagacaca gcuugggauu uuggaagcgu aggaggagca     1860

uuaaacucau uagggaaagg uauacaucaa auuuucggag cagcauuuaa aagucuauuu     1920

gggggaaugu caugguuuag ucaaauauua auagguacau ugcuaaugug guuaggacua     1980

aauacuaaaa auggaaguau aaguuuaaug uguuuagcac uuggaggagu acuaauauuu     2040

uuaagcacag caguaagugc a                                               2061


<210>  2638
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short CDS opt

<400>  2638
accucaaucg guauuauagg acuacuauua acaacagcaa uggcggccga aauaacgaga       60

agaggaucag cauauuacau guaucuagac cgaagugaug caggaaaagc aauaucuuuu      120

gcuacaacac uaggaguaaa caaaugucau guacaaauua uggacuuagg acauaugugu      180

gacgcgacaa ugucauacga augucccaug uuagaugaag gaguugaacc agaugaugua      240

gacuguuggu guaacacaac caguacuugg guaguauacg guacaugcca ucacaagaaa      300

ggagaagcaa gaagaaguag acgagcagug acauuaccaa gccacucaac gagaaaauua      360

caaacaagaa gucaaacaug gcuagaaagu agagaauaca cuaaacaucu aauaaaagua      420

gaaaauugga ucuucagaaa uccaggauuu gcauuaguag caguugcaau ugcaugguua      480

uuaggaucau caaccagcca aaaaguuauu uauuuaguaa ugauccuauu aauugcacca      540

gcauacagua uuagaugcau cggcguuuca aauagagauu uuguugaagg uaugucagga      600

ggaacauggg uagauguagu auuagaacau gguggaugug uaacuguuau ggcacaagau      660

aaaccuacag uggacauuga auuaguaaca acaacaguau ccaacauggc agaaguuaga      720

ucauacugcu augaagcaag cauaagugac auggcuucag auucaagaug uccuacacaa      780

ggagaagcau aucuggacaa acaaucagau acucaauaug ucugcaaaag aacauuaguu      840

gauagaggcu ggggaaaugg gugcggcuua uucggaaaag guagucuagu cacaugcgcu      900

aaauuuacau guaguaagaa aaugacagga aaaucaaucc agccagaaaa cuuagaauac      960

agaauuaugu ugucaguaca uggaucucaa cauaguggua ugauuguaaa cgauacagga     1020

uacgaaacag augaaaauag agccaaagua gaaguuaccc cuaacucacc aagagcagaa     1080

gcaacucuag gaggauuugg auccuuagga cuagauugug aaccaagaac aggcuuagau     1140

uuuucagauc uauauuaccu uacaaugaac aauaaacacu gguuaguaca uaaagaaugg     1200

uuccacgaua uaccacuacc auggcacgca ggagcggaca ccgguacacc acacuggaau     1260

aauaaagaag cauuaguuga guucaaagau gcucaugcca aaagacaaac aguaguaguu     1320

uuaggaucuc aagaaggagc aguacauaca gcuuuagcag gagcauuaga agcagaaaug     1380

gacggagcaa aaggaaaauu auuuucagga cauuuaaaau gcagauuaaa aauggacaaa     1440

uugagauuaa aaggcguaag uuauucacua uguacggcag cauucacauu uacuaaaguc     1500

ccagccgaaa cacugcaugg aacaguuaca guagaaguuc aguacgcagg cacagaugga     1560

ccaugcaaaa uaccuguaca aauggcagua gauaugcaaa cccuaacacc agucggaaga     1620

cuuauaacug ccaauccagu uauaacagaa ucaacugaaa acuccaaaau gauguuagaa     1680

uuagauccac cauuuggaga caguuauaua guaauuggag uaggagacaa gaaaauuaca     1740

caucacuggc aucgaagugg aaguacaaua ggaaaagcau uugaagcuac aguaagagga     1800

gcuaaaagaa uggcaguacu aggugauaca gcaugggauu uugguucugu aggaggugua     1860

uuuaauaguc uuggaaaagg aauacaucaa auauuuggag ccgcauuuaa aucauuauuu     1920

ggaggaaugu caugguucuc acaaauacua auuggcacau uauuaguaug gcuaggacua     1980

aacaccaaaa acggaagcau aaguuuaaca ugucuagcac uaggaggagu aaugauauuu     2040

cuaaguacag caguaagcgc a                                               2061


<210>  2639
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short CDS opt

<400>  2639
acaucaauag gaauuauugg ucuacuucua acaacagcaa uggcugcaga aauaacaaga       60

agaggaagug cauauuacau guauuuagac agaucagacg cuggaaaagc gaucaguuuc      120

gcaacaacuu uaggaguaaa uaaaugucau guacaaauaa uggauuuagg ucacaugugu      180

gacgcaacua uguccuauga auguccaaug uuagacgaag gaguugaacc agaugauguu      240

gauuguuggu guaauacaac aucuacaugg gucguauaug gaacauguca caggaaaaaa      300

ggggaagcaa gaagaucuag aagggcagua accuuaccaa gucauucaac aagaaagcua      360

caaacaagaa gccaaacaug gcuagaaagu agagaauaca cuaaacacuu aauuaaagua      420

gaaaauugga uauuuagaaa uccaggauuc gcauuaguug caguagcaau agcaugguua      480

uuaggcucgu caacaaguca aaaaguuauc uauuuaguaa ugaucuuacu aauagcucca      540

gccuauucaa uuaggugcau cggcguauca aauagagacu uuguagaagg aaugagcgga      600

gguacauggg uagacguagu auuagaacau ggaggaugcg uaacaguuau ggcucaagau      660

aaaccaaccg uagauauaga acuaguaaca acaacaguua guaauauggc agaaguuaga      720

agcuauugcu augaagcguc uauaucagau auggcaucag auaguaggug uccaacacag      780

ggagaagcau acuuagacaa acaaucggau acucaauaug uauguaaaag aacauuagua      840

gauagaggcu gggguaacgg augcggauua uucggaaaag gaucauuagu gaccugugcu      900

aaauuuaccu gcucaaaaaa aaugacgggc aaaucaauac aaccugaaaa ucuagaauac      960

cgcauaaugc uuaguguaca uggaucccag cauucaggaa ugauaguaaa cgauacaggc     1020

uacgaaacag augaaaauag agcaaaggua gaaguuacac cuaauucacc acgagcagaa     1080

gcaacauuag gaggauuugg aagucuaggu uuagauugug aaccaagaac aggauuagau     1140

uuuucagauc uauauuauuu aacaaugaac aauaaacauu ggcuaguaca uaaagaaugg     1200

uuucaugaua uaccauuacc uuggcaugca ggugcagaua caggaacacc ucauuggaau     1260

aauaaagaag cccuugucga guucaaagau gcacacgcca aaagacaaac uguagucgua     1320

cuaggaaguc aagaaggugc aguacacaca gcuuuagcag gagcacucga agcagaaaug     1380

gacggagcga aaggaaaacu guucagcgga cauuuaaaau gccgauuaaa aauggacaaa     1440

uugagacuaa aaggaguauc auacucuuua uguacagcag cauuuacauu uacaaaaguu     1500

ccagcagaaa cauuacaugg aacaguaacc guagaaguac aauaugcagg aacugacggu     1560

ccaugcaaaa uaccaguuca aauggcagua gacaugcaaa cauuaacucc aguggguagg     1620

cuaauuacag caaacccugu aauaacagaa agcacggaaa acucuaaaau gauguuagaa     1680

cuagauccac cauuuggaga uucauacaua guaauuggag uaggagauaa aaaaauuaca     1740

caucauuggc auagaagugg aucaacaaua ggaaaagcau ucgaagcaac uguacgaggg     1800

gcaaaaagaa uggcaguacu aggcgacaca gcaugggauu uuggaagugu ugggggugua     1860

uuuaauucuu uaggaaaagg aauacaucaa auauuuggag cggcauucaa aucauuauuu     1920

ggaggaauga gcugguuuag ucaaauuuua auagguacuu ugcuaguaug guuaggauua     1980

aauacaaaaa acggaucaau aagccuaacc uguuuggcuu uaggaggagu aaugauauuc     2040

uuaagcacag cagucagugc a                                               2061


<210>  2640
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short CDS opt

<400>  2640
accagcauag gaauaaucgg acuguuauua acgacagcua uggcagcuga aauaacaaga       60

aggggauccg cguacuacau guacuuagau agaucugaug cuggaaaagc aauaucauuc      120

gcuacaacac ucggaguaaa caaaugucau guacaaauaa uggaucuagg acacaugugu      180

gaugccacaa uguccuacga auguccuaug uuagaugaag gaguugagcc agaugaugua      240

gauugcuggu guaauacaac uuccacaugg guaguauaug gcacaugcca ucacaagaaa      300

ggagaagcua gaagaaguag gagagccgua acacuaccaa gccauaguac aaggaaauua      360

caaacaagaa gucagacuug gcuagaaagu agagaauaua caaaacaucu gauaaaagua      420

gaaaauugga uauucagaaa uccaggauuu gcucuaguag caguagcaau agcaugguua      480

uuaggaagcu caacaagcca aaaaguuauc uauuuaguaa ugauauuauu aauagcucca      540

gcguauucca uaagaugcau aggaguaagu aauagagacu uuguagaagg aaugaguggu      600

gggacauggg uagauguugu cuuagaacau gggggaugug uaacagugau ggcucaagac      660

aaaccaacag uugacauaga gcuaguaacu acaacaguaa guaacauggc agaaguaaga      720

ucauacuguu augaagccuc cauaucagau auggcauccg acagucgaug uccaacacaa      780

ggugaagcau auuuggacaa acaaagugau acacaguaug uaugcaagag aacauuaguu      840

gauagaggau gggguaacgg guguggauua uuuggaaaag guucacuggu gacaugugca      900

aaauuuacau gcucaaagaa aaugacugga aaaagcauac aaccagaaaa uuuagaauau      960

agaauuaugu uaucaguuca cggaagccaa cauaguggaa ugacuguaaa ugacauagga     1020

uaugaaacag acgaaaacag agcaaaaguu gaaguaacgc caaauucacc uagagcagaa     1080

gcuacauuag gaggauuugg caguuuggga uuagauugug aacccagaac aggacuugac     1140

uucagugauu uauauuaucu gacaaugaac aauaaacauu gguuaguaca uaaagagugg     1200

uuucaugaua uaccauuacc guggcaugca ggagcagaca caggaacucc acacuggaac     1260

aauaaagaag cucuaguaga auuuaaagac gcacacgcua aaagacaaac aguaguaguc     1320

uuagggucac aagaaggcgc aguacacaca gcuuuagcag gagcauuaga agcagaaaug     1380

gacggcgcca aaggcaaacu auuuucuggc cauuuaaagu guaggcuaaa aauggacaag     1440

cuaagauuaa aagggguauc auauucauua ugcacagccg ccuuuacauu uacaaagguu     1500

ccagcagaaa cacugcaugg aacagugaca guagaagucc aauaugcagg aacagaugga     1560

ccauguaaaa uaccaguaca aauggccgua gacaugcaaa cauuaacacc aguaggaaga     1620

cucauaacag caaacccugu cauuacagaa agcacagaaa auucuaaaau gaugcuagaa     1680

uuagauccac cuuuuggaga uucuuauauc gugauaggag uuggagauaa gaaaauaaca     1740

caucauuggc auagauccgg aaguacaaua ggaaaagcgu uugaagccac aguaagggga     1800

gcaaaaagga uggcaguacu cggagauaca gcgugggauu uugggucagu uggaggaguu     1860

uuuaauaguc uaggaaaagg uauacaccaa auauuugggg cagcauucaa aucuuuauuc     1920

ggaggaauga gcugguuuuc acaaauauua auaggcacac uacuuguaug guuaggauua     1980

aauaccaaaa auggaucaau aaguuugaca ugccuagcgc uaggaggggu aaugauauuu     2040

cugucaacag caguaagcgc a                                               2061


<210>  2641
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short CDS opt

<400>  2641
acaaguauag guauaguagg ccuucuacua acgacagcaa uggcagcuga aauaacaaga       60

agagguucag cauauuauau guaucuagau agaagugacg caggaaaagc uauuagcuuc      120

gcaacuacuu uaggaguuaa uaaaugucau guacaaauaa uggacuuagg acauaugugc      180

gaugcaacaa ugaguuauga augcccaaug uuagaugaag gaguagaacc agacgaugua      240

gauugcuggu gcaauacuac aucgacaugg guaguguacg gcacauguca ucacaagaaa      300

ggagaagcga gaagaucaag aagggcagua acacuaccaa gccauaguac gagaaaauua      360

caaacacgau cucaaacaug guuagaauca agagaauaua caaaacacuu aauaaaaguc      420

gagaacugga uauuucggaa uccaggauuc acccucguug caguagccau agcuuggcua      480

cuaggauccu caacuagcca aaaaguaauu uauuuaguaa ugauauuauu aauagcucca      540

gcauauucaa uaagauguau agguguaucu aacagagauu uugucgaagg caugucagga      600

ggaacauggg uagauguagu ucuagaacau ggaggaugcg uaacaguuau ggcacaagac      660

aaacccacug uagauauaga auuaguaacg acaacaguau caaauauggc agaaguacga      720

agcuauugcu augaagccuc aauaucagac auggcuucag auucaagaug uccaacacag      780

ggugaagcau aucucgacaa acaaucggac acacaauaug uauguaaacg cacauuagua      840

gauagaggau gggguaaugg augcggucua uucggaaaag gaucauuagu aacaugugca      900

aaauucacuu gcucuaagaa aaugacggga aaaucaauuc agccagagaa uuuagaauac      960

cguauaaugu uaucuguuca uggaucacaa cauucuggga ugauaguaaa ugaugaaaau     1020

agagcaaaag uagaagugac accaaauuca ccaagagcag aagcuacacu aggaggauuu     1080

ggaucacuag gauuggauug cgaaccacga acuggcuuag auuuuucaga ucuauacuau     1140

uuaacaauga auaacaaaca uuggcuagua cauaaagaau gguuccacga uauaccauua     1200

ccauggcaug cuggggcaga uacuggaaca ccacauugga auaauaagga agcauuagua     1260

gaauuuaaag augcucacgc aaaaagacaa acaguagucg uguuaggaag ucaagaagga     1320

gcaguacaua ccgcauuagc uggugcacua gaagcagaaa uggauggagc aaaaggaaga     1380

uuauuuucug gacaucuaaa augccgauua aagauggaua aacuuagauu aaaagguguu     1440

agcuacaguc uauguacagc agcauucaca uucaccaaag uaccugcaga gacccuacac     1500

ggaacaguua ccguagaagu acaauaugca gggacugaug gaccaugcaa aguaccugca     1560

caaauggcag uagacaugca aacauuaacc ccaguaggaa gauuaauaac agcuaaucca     1620

guaauaacag aaucaacaga aaacagcaaa augaugcuag aauuggaccc accuuuugga     1680

gauaguuaca uaguaauagg aguaggagau aagaaaauaa cacaucacug gcauaggagu     1740

gguaguacaa uuggaaaagc auuugaagca acaguuagag gagcuaaaag aauggcaguc     1800

uuaggagaua cagcauggga uuuuggaagu guaggaggug uuuuuaauuc guuaggaaaa     1860

ggaauacauc aaauauuugg agccgcuuuc aaaagucuuu uuggaggaau gucaugguuu     1920

ucacaaauuc uuauagggac auuauuaguu uggcuuggau uaaacacuaa aaacggaagu     1980

auaucuuuaa caugccuagc acuaggaggg guaaugauuu uucuaaguac agcaguaagu     2040

gca                                                                   2043


<210>  2642
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short CDS opt

<400>  2642
acgucaauug guauaauagg ccuauuacua acaacagcua uggcggcaga aauuacaaga       60

aggggaucag cauauuauau guaccuagau agaucagaug cagguaaagc uauaaguuuu      120

gcuacuacau ugggaguaaa uaaaugucac guacaaauaa uggaucuggg ucauaugugc      180

gacgcuacaa ugucguauga auguccaaug cuagacgaag gggucgaacc ugaugaugua      240

gauuguuggu guaauacuac aucgacaugg guaguauaug gcaccuguca ucacaaaaaa      300

ggagaagcaa gaagaucaag aagggcagua acacuaccaa gucauagcac uagaaaauua      360

caaacaagaa gccaaacaug guuagaaagc cgagaauaua caaaacauuu aauaaaagua      420

gagaauugga uauuuagaaa uccaggauuu gccuuaguag caguugcaau agcauggcua      480

cuuggaucau ccacuaguca aaaaguaaua uaucuaguaa ugauauuauu aauagcacca      540

gcauauagua uuagauguau aggaguuuca aauagagacu uuguugaagg uaugucugga      600

ggaacauggg uagacguagu cuuagaacau ggaggaugug uaacaguaau ggcacaagau      660

aaaccuacag uagauauaga acuaguaaca acaaccguua guaauauggc agaaguaagg      720

ucauauuguu acgaagcauc aauaucugac auggcaagug auagucgaug cccaacacaa      780

ggugaagcgu auuuggauaa acaaucagau acacaauaug uauguaaaag aacucuagua      840

gauagagguu ggggcaacgg cuguggauua uuuggaaagg gaagccuagu aacaugugca      900

aaauucacuu gcaguaaaaa aaugacagga aaaucaauac aacccgagaa ccuagaauau      960

agaauaaugu uaucaguaca ugggucgcag cacucaggaa ugauaggaua ugaaacagau     1020

gaaaauaggg cuaaaguuga gguuacgcca aauucuccac gcgcagaagc aacauuaggu     1080

ggauucggcu cacuaggacu agauugugag ccaagaacag gauuagacuu uagugaucua     1140

uauuaucuaa caaugaauaa uaaacauugg uuaguacaua aagaaugguu ucaugacaua     1200

ccauuaccau ggcacgcagg agcugauaca ggcacuccgc auuggaauaa uaaagaagca     1260

cucguagagu uuaaagacgc acacgccaaa cgacaaacag ugguuguacu aggaucacaa     1320

gaaggagcag uucacacagc acuagcagga gcacuagaag ccgaaaugga uggagcuaaa     1380

gguaaauuau uuaguggaca ucugaaaugc agacuaaaaa uggacaaacu uagauugaaa     1440

gguguaaguu auaguuuaug caccgcagcc uuuacuuuua caaaaguacc agcagaaaca     1500

cuucauggca caguaacagu ugaaguacaa uaugcuggua cagauggacc uuguaaaauu     1560

ccuguacaaa uggcagucga caugcaaaca uuaacaccag uuggacgccu aauaacggcu     1620

aauccaguaa uaacugaaag cacagagaau aguaaaauga uguuagaauu agauccccca     1680

uucggagaua guuacauugu aaucggaguu ggagauaaaa aaauaacgca ucauuggcac     1740

agaagcgguu caacaauagg aaaagcauuu gaagcaacag ucagaggugc gaaaagaaug     1800

gcaguccucg gcgauacagc augggauuuc gguucagugg gaggaguauu caauucucua     1860

gggaaaggua uacaucaaau auuuggugca gccuuuaaau cauuauuugg aggaaugagu     1920

ugguuuuccc aaauauuaau uggaacauua uuaguguggu uagggcucaa cacaaaaaac     1980

ggaaguauau cacuaacuug uuuagcacua ggaggaguaa ugauauuuuu gucaacugcu     2040

guaagcgcu                                                             2049


<210>  2643
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short CDS opt

<400>  2643
acaagcguag gaauaguagg ucuauuacua acgacagcaa uggcagcaga aguaacuaga       60

agaggaucgg cauauuauau guauuuagac agaaaugaug cgggggaagc aauaucuuuu      120

ccgacaacau uaggaaugaa uaaauguuau auacaaauca uggaccuugg acauaugugu      180

gacgcgacaa ugucauauga auguccaaug uuggaugaag gaguagaacc agaugauguu      240

gauuguuggu guaacacgac aucuacaugg guaguauaug gcacuugcca ucauaaaaaa      300

ggagaagcga gaagaagcag aagagcagug acacuaccua gucauucaac aagaaaauua      360

caaacaagau cacaaacaug guuagaauca agagaauaca caaaacacuu aauucgaguu      420

gaaaacugga uauucagaaa cccaggauuu gccuuagcug cugcagcaau agcaugguua      480

uuaggcagua guacaagcca aaaaguaaua uaucuaguaa ugauccuauu aauagcccca      540

gcauauagua uaagauguau ugguguaucu aaucgagauu uuguagaagg aaugucagga      600

ggaacauggg uagacguagu auuagaacac ggaggaugug ucacaguaau ggcacaagac      660

aaaccaacag uagauauuga auuagucaca acuacaguaa gcaauauggc agaaguaaga      720

aguuauuguu augaagccuc cauaucagau auggcaagug acucaagaug uccgacucaa      780

ggagaagcuu aucuugauaa acaaagcgau acucaguacg uaugcaaaag aacacuuguu      840

gauagaggcu ggggaaacgg augcggauua uucggaaaag gaagccuggu aacaugcgcu      900

aaauuugcau guaguaaaaa aaugacagga aaaagcauac aaccagaaaa ucuagaauau      960

cgaauaaugu uaucaguaca cggcucacag cauucaggaa ugauuguuaa cgacacagga     1020

cacgaaacug acgagaauag agcaaaaguu gaaauaacac caaacucacc gagagcagaa     1080

gcaacauuag gaggcuucgg cagccuagga cuagacugug aaccacgaac aggcuuagac     1140

uuuagugauc uauauuaccu cacuaugaau aacaaacauu gguuaguuca caaagaaugg     1200

uuccaugaua uaccuuuacc auggcaugca ggugccgaua cugguacacc acacuggaac     1260

aacaaagaag cauuaguaga auuuaaagau gcacaugcaa aaagacaaac aguuguugua     1320

uuaggaucac aagaaggagc aguacacaca gcguuagcag gggcacuaga agcagaaaug     1380

gacggggcca aaggacggcu aaguucagga cauuuaaaau guagauuaaa aauggauaaa     1440

uuaagacuaa aaggaguauc guacucgcug ugcacagcag ccuuuacauu cacaaaaauu     1500

ccugccgaaa cacuacacgg aacuguaaca guagagguac aauacgcagg uacugaugga     1560

ccauguaaag uaccagcgca aauggcagua gauaugcaaa cauugacacc aguaggaaga     1620

uuaauaacag caaauccagu aauaacagaa ucuacagaaa acucaaaaau gaugcuugaa     1680

uuagauccac cuuuuggaga uaguuacaua guaauagggg uaggagaaaa aaagauaacc     1740

caccauuggc auagaucagg aucaaccaua gggaaagcau uugaagccac ugucagagga     1800

gcuaaaagga uggcaguuuu aggagacaca gcaugggauu ucggaagcgu ggguggcgca     1860

cucaauaguc uuggacgagg aauacaucaa auauuuggug ccgcauucaa aagucuuuuu     1920

ggaggaauga guugguuuuc ucagauacua auuggaacau uauuaaugug gcuaggauua     1980

aauacaaaaa auggaaguau uucauugaug uguuuagcac uaggaggagu auuaauauuu     2040

cuaaguacgg caguaagugc a                                               2061


<210>  2644
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short CDS opt

<400>  2644
acaagugucg gaauaguggg auuacuuuua acaacagcaa uggcugcuga aguaacuaga       60

agagguucag cauacuacau guacuuagac agaaaugaug caggagaagc aauaaguuuu      120

ccgacaacau ugggaaugaa uaaauguuau auacaaauaa uggauuuagg acauaugugu      180

gaugcaacaa ugucauauga augucccaug uuagacgaag gcguagaacc agaugaugug      240

gauugcuggu gcaauacaac aucaacuugg guagucuacg gaacauguca ucacaagaaa      300

ggugaagcaa gaagaagcag acgagcagua acauuaccua gucauagcac uagaaaguua      360

caaacacgga gucaaacuug guuagagagu agggaauaua caaagcauuu aauaagagua      420

gaaaacugga uauucagaaa uccaggucua gcucuagcug cagcugcaau ugcauggcua      480

uuaggaucaa guaccucaca aaaagugaua uauuuaguaa ugauuuuacu aaucgcacca      540

gcauauucaa uaagaugcau aggaguuagc aaucgagauu ucguagaagg aaugagugga      600

ggaacauggg uagauguugu auuagaacac gguggaugug uuacagugau ggcgcaagau      660

aaaccuacag uagauauaga auuaguuaca acaacuguua gcaauauggc ggaaguaaga      720

aguuauuguu acgaagccag uauaagugau auggcaucgg acaguagaug ccccacacaa      780

ggugaagcau aucuagauaa acaaucagau acacaauaug uuugcaaaag aacauuagua      840

gacagagguu ggggaaaugg uugcggucuu uuuggaaaag guagucuagu aacaugcgca      900

aaauucgccu guagcaagaa aaugacaggu aaaaguauac agccugaaaa cuuagaguau      960

agaauaaugc uaucaguaca cgggagccaa cacagcggaa ugauuguuaa cgacacggga     1020

caugaaacag augaaaacag agcaaaagua gaaauuacac cgaauagccc ucgagcagaa     1080

gccacacuag gugguuuugg aagccuagga uuggacugug aaccaaggac aggucuagau     1140

uuuagugauc uauauuacuu aacaaugaac aauaaacauu gguuaguaca uaaagaaugg     1200

uuucaugaca ucccuuuacc auggcaugca ggagcagaua ccggaacucc acauuggaac     1260

aauaaagaag cauuaguaga auuuaaagau gcacacgcaa agagacaaac aguaguagua     1320

uuaggaagcc aagaaggagc cguacauaca gccuuagcag gcgcguuaga agcagaaaug     1380

gauggagcaa aggguagauu aaguucggga cauuugaaau guaggcucaa aauggauaaa     1440

uuacgauuga aaggaguaag uuacucuuug uguacagcag cuuuuacuuu uacaaaaaua     1500

ccagcagaaa cauuacaugg uacagucacu guggaaguac aauaugcagg aacagaugga     1560

ccaugcaaag uaccugcaca aauggcagua gacaugcaaa cacuuacacc gguagguaga     1620

uuaauaacag cuaauccagu aauaaccgaa aguacagaaa acagcaaaau gauguuagaa     1680

uuagacccgc cauuuggaga uucauauaua guaauuggag uaggagaaaa gaaaauaaca     1740

caccauuggc auagaucagg aaguacuaua gguaaagcgu ucgaagcuac aguaagagga     1800

gcaaaaagaa uggccguguu gggcgacacu gcaugggacu uuggaucggu uggaggugca     1860

cuaaauucau uagguaaagg cauucaccaa auauuuggag cagcauuuaa gucauuauuu     1920

ggaggaauga gcugguuuuc acaaauacua auuggaacgu uauuaaugug gcuaggauua     1980

aauacuaaaa augguagcau uaguuuaaug ugucuagccu ugggaggagu guuaaucuuc     2040

cuuaguacag caguuagugc u                                               2061


<210>  2645
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short CDS opt

<400>  2645
acaucaguag guaucguagg auuacuauua acaacagcaa uggcagcaga aguuacgaga       60

agagguucag ccuacuauau guaucuugau aggaacgaug caggggaagc uaucaguuuu      120

ccuacaacau uaggaaugaa caaaugcuau auucaaauaa uggauuuagg acauaugugu      180

gacgcuacua ugagcuauga auguccuaug cuagacgaag gcguagaacc ugaugacgua      240

gacuguuggu guaauacaac aucgaccugg guaguguaug gaacguguca ucauaagaaa      300

ggagaagcaa gaagaagcag acgagcugua acauuaccau cacauaguac aagaaaauua      360

caaacacgau cacaaacaug gcuagaaagu agagaauaua caaaacauuu aauuagaguu      420

gaaaauugga uauuuaggaa uccagguuuu gcauuggcag cagcugcaau ugcguggcug      480

cucggcaguu caacaucaca aaaaguaaua uaucuaguaa ugauauugcu aauagcucca      540

gcuuauucaa uaagaugcau aggaguuuca aauagagacu uuguugaagg caugagugga      600

ggcacauggg uagacguagu auuagaacac ggaggaugcg ugacuguaau ggcacaagau      660

aaaccaacag uagauauuga guugguaaca acaacagugu caaauauggc agaaguaaga      720

aguuauuguu acgaagcuag cauaagugau auggcaucag acagcagaug uccuacucaa      780

ggcgaagcau aucucgacaa acaaagugau acacaauacg uauguaaaag gacacugguc      840

gacagagggu ggggaaacgg cugugguuug uuuggaaaag gaagucuagu aaccugugca      900

aaauuugcuu guaguaaaaa gaugacggga aagaguaucc aaccagaaaa cuuggaauau      960

agaauaaugu uauccgugca cgggagucaa cauucaggaa ugauaguaaa cgauacagga     1020

caugaaacag acgaaaauag agcaaaagug gaaauuacuc caaauagucc aagggccgaa     1080

gcuacucuug gcggauuugg aucauuagga uuagacugcg aaccgaggac aggacuagau     1140

uucagugauu uguacuaucu aacaaugaac aacaaacauu gguuagucca uaaagaaugg     1200

uuccaugaua uuccauuacc guggcaugcu ggugcggaua cuggaacacc acauuggaau     1260

aauaaagaag cauuaguaga auuuaaagau gcucaugcaa aaagacaaac cguaguaguu     1320

uuagguaguc aagaaggagc aguacacaca gcauuggcgg gagcccuaga agcagaaaug     1380

gauggagcaa aagggagacu aucuucagga cacuugaaau guagauugaa aauggauaaa     1440

cucagauuaa aagggguauc auauucauua uguacagcug cauucacauu uacaaaaaua     1500

ccagcugaaa cacuacaugg uacuguuacg guagaaguac aauaugcagg cacagacgga     1560

ccuuguaaag uuccagcaca aauggcagua gacaugcaaa cauuaacacc uguaggaaga     1620

uuaauaacag caaauccagu gauaacagaa ucaacagaaa auaguaaaau gauguuagaa     1680

uuagauccac cauuuggcga uucauauaua guaauaggug uaggagaaaa gaaaaucacc     1740

caccauuggu auagaagcgg aagcacaaua gguaaagcau uugaagcaac aguaagagga     1800

gcaaaaagga uggccguucu aggagacacu gcaugggauu uuggaagugu aggaggagca     1860

cuaaacucuu uaggaaaagg uauucaucaa auauuuggag cagcauuuaa aucguuauuc     1920

ggaggaauga gcugguuuuc acaaaucuua auaggaacau uacuaaugug gcuuggauug     1980

aauaccaaaa auggaucgau aucuuuaaug ugucuagcac uagggggagu uuuaauauuu     2040

cuaucaacag cagugagugc a                                               2061


<210>  2646
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short CDS opt

<400>  2646
acgucuguug gaauaguugg auuauuacua acaacagcaa uggcagcaga aguuacaaga       60

agaggcucag ccuauuacau guacuuagau agaaaugacg caggagaagc uauuagcuuu      120

ccgacuacuc uaggaaugaa uaaaugcuac auacaaauaa uggacuuagg acacaugugc      180

gaugcaacaa ugaguuacga auguccaaug cuagacgagg guguugaacc ggaugaugua      240

gauuguuggu guaacaccac aagcacuugg gugguauaug gaacguguca ccacaaaaaa      300

ggagaagcac guagaucacg aagggcagua acacucccau cacauaguac uagaaaauua      360

caaacaagaa gucaaacgug guuagaauca agagaauaca ccaaacacuu aauuagagua      420

gaaaauugga uauuucgaaa uccagguuuu gcucuagcag cagcagcaau agcaugguua      480

uuaggaagcu cgacaaguca aaaaguaaua uauuuaguaa ugauacuauu aauagcuccg      540

gcuuauucaa uaagauguau aggaguauca aauagagacu uuguagaagg aaugucugga      600

ggaacuuggg uugauguggu auuagaacau gggggaugug uaacaguaau ggcccaagau      660

aaaccaacag uugauauaga acuuguuaca acaaccguaa guaacauggc cgaaguaaga      720

uccuauugcu augaagcauc aauaucggau auggcaagug auucaagaug uccuacucaa      780

ggagaagccu acuuagacaa acaaagcgau acacaauaug uaugcaaaag gacacuagua      840

gaucgugguu ggggaaacgg auguggauug uucggaaaag guagccucgu aacaugugca      900

aaauuugcuu guaguaaaaa aaugacagga aaaucaauac aacccgaaaa uuuagaauau      960

agaauaaugc uaagugucca cgggagccaa cauagcggua ugauaguuaa cgauacagga     1020

caugaaacug augaaaauag ggcaaaaguc gaaauaacac caaauagucc aagagcagaa     1080

gcaacauuag guggauuugg aucuuuagga cuagacugug aaccaaggac ugguuuagau     1140

uuuagcgacu uauauuaucu aacaaugaac aauaaacauu gguuaguaca uaaagaaugg     1200

uuccaugaua uaccauuacc auggcacgca ggagcagaua ccgguacccc gcacuggaau     1260

aauaaagaag cacuaguuga auuuaaagac gcacaugcaa aacgucagac aguaguagua     1320

uuaggaaguc aagaaggcgc aguacauaca gcauuagcag gagcacuuga agcggaaaug     1380

gauggugcaa aaggaagauu aaguucagga cauuugaagu gucgguuaaa aauggacaaa     1440

cuaagauuaa aaggaguauc auauucgcua uguacagcug cuuuuacauu cacuaaaauc     1500

ccagcugaaa cacuacaugg aacaguaaca guagaaguuc aauaugcagg uacugacgga     1560

ccaugcaaag ucccagcaca aauggcagug gauacacaga ccuuaacacc cgugggaaga     1620

cuuauuacug caaauccagu aauaacugaa ucaacugaaa auucaaaaau gauguuagaa     1680

uuagauccac cauuuggaga cucguauauc guaauuggug uaggagaaaa aaaaauaacu     1740

caucacuggc auagaucagg aucaacaaua ggaaaagcau ucgaagcuac aguaagaggc     1800

gcaaaaagaa uggcaguccu aggagauacu gcaugggacu ucggaagcgu aggaggcgca     1860

cuuaauucuc uagguaaagg aauacaucaa auauuuggag cagcauuuaa aagcuuauuu     1920

ggaggaaugu ccugguuuuc acaaauacua auaggaacuu uguuaaugug gcuuggucuu     1980

aauacaaaaa augggucaau aaguuuaaug ugccuagcuu uaggaggggu cuuaauauuu     2040

cuuuccacug caguaagugc a                                               2061


<210>  2647
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short CDS opt

<400>  2647
acaucaguug gcauuguagg auuacuauua acaacagcua uggcagcaga aguaacacga       60

aggggaucag cauauuauau guaucuagau agaaacgaug caggagaagc aaucagcuuc      120

ccaacaacac uagggaugaa uaaauguuau auacaaauua uggauuuggg ccacaugugu      180

gacgcaacaa ugaguuauga augcccaaug cuugacgaag gaguugaacc cgaugaugua      240

gacugcuggu guaauacaac cucaacuugg guaguuuaug gaaccuguca ucacaagaaa      300

ggagaagcuc gaagaucaag acgagcagua acauuaccua gucauucaac gagaaaauua      360

caaacuagau cacaaacuug gcuugaaucc agagaauaca caaaacaucu aauaagaguu      420

gaaaacugga ucuuuagaaa cccugguuuu gccuuagcag cggcugcaau agcaugguua      480

cuaggaucau caacaaguca gaaagucaua uauuuaguca ugauauuacu aauagcuccu      540

gcauacucua uaagauguau aggugugagu aauagagacu uuguggaagg aaugucuggu      600

ggaacauggg uggacguagu uuuagagcac gggggaugcg uuacaguaau ggcccaagau      660

aaaccaacag uugauauuga gcuaguaaca acgaccguaa gcaauauggc agaaauaaga      720

ucauauugcu augaagccuc aauuagugac auggcuagcg auagcagaug cccaacacag      780

ggagaagcau accuggacaa acaaucagac acacaauaug uaugcaaaag aacauuagua      840

gacagaggau ggggaaaugg uuguggacua uuuggaaaag ggucauuagu aacaugcgca      900

aaauuugcau guucaaagaa aaugaccggu aaaucaauac aaccagaaaa uuuagaauac      960

agaauaaugc uaucaguaca cgguagucaa cauagcggga ugauuguaaa cgauacagga     1020

cacgaaacag augaaaaucg cgcaaaagua gaaauaacgc caaauagucc uagagcugaa     1080

gcaacauugg guggauuugg gaguuuagga cuagauugcg aaccuagaac aggacuagau     1140

uuuagugacu uauauuaucu cacaaugaau aauaaacacu gguuaguuca caaagaaugg     1200

uuucaugaua uaccauuacc auggcaugca ggagcagaua cagguacacc ccauuggaac     1260

aauaaagaag cucuaguaga guuuaaagac gcacaugcaa aacgacaaac aguaguaguu     1320

uuagguaguc aagaaggcgc agugcauaca gcacuagcag gagcauuaga agcagaaaug     1380

gacggugcga aaggucgauu aaguagugga cauuuaaaau gccgacuaaa aauggacaaa     1440

uuacgauuaa aagguguauc auacucauua uguaccgcug cauucacuuu uacaaaaaua     1500

ccugcagaaa cauugcacgg aacuguaaca guagaaguac aauaugcggg uacagaugga     1560

ccaugcaaag uuccagccca aauggcugug gauaugcaaa cacuaacacc aguagguaga     1620

cuuauaacgg caaauccagu aauaacagaa aguacagaaa auucaaaaau gauguuagaa     1680

uuagaucccc cauuuggaga cagcuauaua guuauaggag uaggagagaa aaagauaacu     1740

caucauuggc aucgaagcgg uucaacaauu ggaaaagcau uugaagcaac uguaagagga     1800

gcaaaaagaa uggccgucuu aggggacacu gcuugggauu ucggaucagu aggcggugca     1860

uuaaauucac uaggaaaagg uauucaccaa auauuuggag cagcauucaa aucauuauuc     1920

ggaggcauga guugguucag ucaaauacua auaggaacau uacuaaugug gcuuggacua     1980

aacacuaaaa augguucaau aaguuuaaug ugucuugcau uaggcggcgu acuuauauuu     2040

uuaucaacag caguaucagc a                                               2061


<210>  2648
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short CDS opt

<400>  2648
accucaguag guauaguugg auuacuacua acaaccgcaa uggcugcaga aguaacaaga       60

agaggaucag cauauuauau guauuuagac agaaacgaug caggggaagc gauaucuuuu      120

ccaacaacuu uagggaugaa uaaauguuac auacaaauaa uggaccuagg ucacaugugu      180

gaugcaacua ugucauauga augcccaaug cucgaugaag gaguagaacc agacgacgua      240

gacuguuggu guaauacaac uucaacaugg guaguauaug gcacaugcca ucauaagaaa      300

ggagaagcaa gaagaucacg uagagcagua acauugccau cacauucuac cagaaaacua      360

caaacaagaa gccaaacaug guuagaauca agagaauaca caaaacacuu aauaagagua      420

gaaaauugga uuuuuagaaa cccagguuuc gcuuuagcug cagcagcuau agcuuggcua      480

uuagggagua gcacaucaca aaaagucaua uaucuaguua ugauccuccu gauagcacca      540

gcauauagua uacgauguau aggaguauca aacagggacu ucguugaagg aaugagugga      600

ggaacuuggg uagauguugu ucuagagcau ggaggcugug uaacuguaau ggcacaagac      660

aaaccaacag uagacauaga gcuaguaaca acuacuguaa gcaauauggc ugaaguaaga      720

ucuuauugcu augaagcaag cauaucagau auggcaagug acagucgaug uccaacccaa      780

ggagaggcuu auuuagauaa acaauccgau acucaauaug uuuguaaacg aacccuagug      840

gacagaggau gggguaacgg auguggacua uuugggaaag gaagccucgu gacaugugca      900

aaauuugcuu guucgaaaaa gaugacagga aaaucaauac aaccugaaaa ucuagaauau      960

agaauaaugc uaucuguaca cggaagccaa cauaguggaa ugauaguaaa ugacacaggu     1020

caugaaacag acgagaacag agcaaaaguu gaaauuacgc ccaauagccc ucgagcagaa     1080

gcuacacuag ggggauuugg uucucuagga cuagacugug aaccaagaac aggccuagau     1140

uuuucagacc uauauuauuu aacaaugaau aauaaacacu ggcuagucca uaaagaaugg     1200

uuccacgaua uaccauuacc cuggcacgcc ggagcagaca caggaacacc ucacuggaau     1260

aauaaagaag cccuuguaga auuuaaagau gcacacgcaa aacgucaaac cguaguagua     1320

uuaggcaguc aagaaggagc aguucauaca gcauuagcug gagcauuaga agcagaaaug     1380

gacggagcaa agggcagacu auccucagga caucuaaaau gcagauugaa aauggauaaa     1440

cuacgauuaa aaggaguaag uuauucacug uguacagcag cauuuacguu uacaaaaaua     1500

ccagcagaaa cauuacacgg aacagucaca guagaaguuc aauaugcagg aacagacggu     1560

ccauguaaag uauuagcaca aauggcugua gauaugcaaa cacuaacacc uguaggaaga     1620

uuaauuacug caaauccugu uaucacugaa agcacagaaa acucaaaaau gaugcuugaa     1680

uuagauccac cauuuggcga uucauacauc gucauuggag uaggagaaaa gaaaauaacg     1740

caucacuggc accgaagugg aucaacaaua gguaaagcau uugaagcaac aguacgugga     1800

gcuaaaagaa uggcaguuuu aggagauaca gcuugggauu uuggaagugu cgguggagca     1860

cuaaacucac uaggaaaagg aauacaucaa auauuuggag cagcauucaa aucauuauuu     1920

ggaggcaugu caugguuuag ucaaauuuua auaggaacuc uacuaaugug guuaggauua     1980

aacacuaaaa acgguucaau auccuuaaug ugucuagccu uaggaggagu ucuuauauuu     2040

cucagcacag cuguaagugc u                                               2061


<210>  2649
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short CDS opt

<400>  2649
acgaguguag gaauaguagg auuguugcua acaacagcua uggcugcaga aguaacuaga       60

agaggcagug ccuacuacau guacuuagac agaaaugacg cuggugaagc aauuagcuuu      120

ccaacaacac uaggaaugaa caaauguuau auacaaauaa uggacuuagg ccauaugugu      180

gaugcaacca ugucauacga guguccaaca uuagacgagg gcguugaacc agacgacgua      240

gauugcuggu guaauaccac uucaacaugg guaguauaug guacuuguca ccauaagaaa      300

ggugaagcaa gaagaucacg uagagcagug accuuaccuu cacauucuac aagaaaauua      360

caaacaagga gucaaacaug gcuugaauca agagaguaua caaaacaccu aauaaggguu      420

gaaaauugga uauuucgaaa cccgggguuu gcacuagccg cagcugcaau agcauggcuc      480

cuaggaaguu caacuagcca aaaaguaaua uaccuaguaa ugaucuugcu uauagcccca      540

gcauacagua uaagauguau uggagugagu aauagagauu ucguagaagg aaugucagga      600

gggacuuggg uugauguagu auuagaacau ggaggaugcg uaaccguaau ggcacaagau      660

aaaccgacgg uugauauaga auuaguaacc acaacaguaa guaauauggc ugagguaaga      720

aguuacuguu augaggcauc aauaucagac auggccagcg acagucguug uccaacgcaa      780

ggagaagccu auuuagacaa acaaucagau acacaauaug uauguaaaag aacucuaguu      840

gauagaggau ggggaaacgg cugcggacuu uuuggaaaag gaucauuagu gacaugcgca      900

aaauuugccu guaguaagaa aaugacaggu aaaucuauac aaccugaaaa uuuagaauau      960

agaauuaugu uaucaguaca cggaucacaa cauucgggga ugauaguaaa ugauaccgga     1020

cacgaaacag augaaaauag agcuaaaguu gaaaucacac caaacagucc aagagcagaa     1080

gcaacauuag gcggauucgg aucacuaggc cuagauugug aaccaagaac aggacuagau     1140

uuuagcgauc uuuauuacuu aacaaugaau aauaaacauu gguuaguuca uaaagaaugg     1200

uuucaugaua uaccccuucc auggcaugcc ggagcagaua cuggaacacc acacuggaau     1260

aacaaagaag cauuagucga auuuaaggau gcacaugcaa aaagacaaac uguuguagua     1320

uuaggaucac aagaaggagc aguacauaca gcuuuagcug gagcacuaga agcugaaaug     1380

gacggagcaa aaggacgauu aagcucugga caccuaaaau guagacuaaa aauggauaaa     1440

cuaagacuca aaggaguuuc uuauucguua uguacugcag cauuuacuuu uacaaaaauu     1500

ccagcagaaa cgcuacaugg aacaguaacu guagaaguac aauaugccgg aacugaugga     1560

ccauguaaag ucccagcaca aauggcaguu gauaugcaaa cacuaacucc aguaggaaga     1620

uuaauaaccg cuaauccagu cauaacagaa ucaacagaaa auucaaaaau gauguuagaa     1680

uuggauccuc ccuuugguga caguuacaua guaauaggag uaggagaaaa gaaaauaaca     1740

caucacuggc auagaagugg aucaacuaua gguaaagcau uugaagcuac aguacgagga     1800

gcaaaaagaa uggccguucu uggagacaca gcuugggacu uuggaagugu aggaggagcu     1860

cuaaauagcu uaggaaaggg uauacaucaa auauuuggag ccgcuuuuaa aagucuauuc     1920

ggaggaaugu caugguuuuc acaaauauua auaggaacac uacuaaugug guuaggacua     1980

aauacgaaaa auggcagcau uagcuuaaug uguuuagcac uaggaggagu ucuaauauuc     2040

uuaucaacag ccguaagugc a                                               2061


<210>  2650
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short CDS opt

<400>  2650
acaucaguag gaauaguggg auuacuuuua acaacggcaa uggcagcuga aguaacaaga       60

agaggcagug cuuauuauau guauuuagac aggaaugaug caggugaagc aauuaguuuu      120

ccuacaacuu uaggcaugaa caaauguuac auacaaauaa uggaucuagg acauaugugc      180

gaugcaacaa ugucauauga gugcccuaug uuagaugaag guguugaacc agacgaugug      240

gauugcuggu guaauaccac cagcacuugg guaguauacg gaacauguca ucauaaaaaa      300

ggcgaagcaa gaagaaguag gagagcugua acucucccua gucauucuac gagaaaauua      360

caaacaagau cacaaacuug gcuugaauca agagaauaua caaaacauuu aauacgagua      420

gaaaacugga uauuuagaaa uccagguuuc gcacuggcag cagcagcaau agcaugguua      480

cuaggaucau caacaagcca aaaaguaaua uacuuaguaa ugauucuguu aauagcacca      540

gcguauagca uccguuguau agguguauca aauagagauu uugucgaagg aaugagugga      600

ggaacauggg uugauguagu ucuagaacac ggaggcugcg ucacagucau ggcacaagau      660

aaaccaacgg uagauauuga auuaguaaca acaaccguca gcaacauggc agaaguaaga      720

ucauacuguu augaagcauc aauauccgau auggcaucag acucuagaug uccaacucag      780

ggagaggcau auuuagauaa acaaucagau acgcaauaug uauguaaaag aacauuagua      840

gauagagggu ggggcaaugg auguggauua uuuggaaagg gaucuuuagu uacuugugca      900

aaauuugcau gcucaaaaaa aaugacagga aaaaguauuc aaccagaaaa uuuagaauac      960

cgaauaaugc uuaguguaca cggaagucaa cauucaggaa ugaucgucaa ugacacaggg     1020

cacgaaacag acgaaaacag agcaaaagua gaaauaacac cuaauucucc aagagcagaa     1080

gcaacuuuag guggauucgg aagcuugggc cuugauugug aaccaagaac cgggcuugau     1140

uuuagcgauu uauacuauuu aacaaugaau aauaaacauu gguuaguaca uaaagaaugg     1200

uuccaugaua uaccucuacc auggcacgca ggugcagaua caggaacacc acacuggaac     1260

aauaaagaag ccuuaguuga auuuaaagau gcacaugcga aaagacaaac agucgucgua     1320

cuaggaaguc aagaaggagc aguacauacu gcucuugcag gggccuuaga agcagagaug     1380

gauggagcaa aaggaagauu aucuucggga caucucaaau guagauuaaa aauggauaaa     1440

uuaagacuaa aaggugucuc cuacagucua uguacggcag cauucacauu uacaaaaaua     1500

ccugcagaaa ccuuacaugg aacaguaaca gucgaaguac aauaugcagg aacagauggu     1560

ccauguaaag uaccagcaca aauggcaguu gauaugcaga cauuaacacc cguaggaaga     1620

uuaauaacag caaauccagu cauaacugaa ucaacagaaa acaguaaaau gauguuagaa     1680

uuagauccgc cauucggaga uaguuauaua guuauuggag ucggugaaaa aaaaauaacu     1740

caccauuggc acagaucagg aucaacaaua ggaaaagcau uugaagcaac aguaagagga     1800

gcaaaaagaa uggcaguguu aggagauacu gcaugggauu uuggaagugu aggaggagcc     1860

uugaauucau uagguaaagg uauacaccaa auauuugggg ccgcauuuaa aagcuuauuu     1920

ggaggaauga guugguuuuc acaaauucua auaggaacau uauuaaugug gcuuggauua     1980

aauacuaaaa acgguucuau uucacuaaug uguuuagcau uaggcggggu ucuaauauuu     2040

cuuucuacgg ucguuucagc a                                               2061


<210>  2651
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short CDS opt

<400>  2651
acaucaguug guauaguugg acuacuauua acaacagcga uggcagcuga aguaacaaga       60

agaggaucag cauauuacau guacuuagau aggaacgaug cuggugaagc aauuucauuu      120

ccuacaacac uaggaaugaa uaaauguuau auacaaauaa uggaccuagg ucauaugugc      180

gaugcuacua ugucuuauga augcccaaug cuagaugaag gaguagaacc agaugaugua      240

gacuguuggu guaacacaac cagugcaugg guaguauaug guaccugcca ucacaagaaa      300

ggagaagcua gaagaucuag acgagcugua acauuaccua gccauucaac gagaaaauua      360

cagacaagac cacaaacuug gcuagaaucu agagaauaca ccaaacaucu aauaagggua      420

gaaaauugga uauuuagaaa uccagguuuu gcacuagcug ccgcagcaau ugcuugguua      480

cuagguucau caacaucuca aaaaguaauc uaucuaguua ugaucuuacu aauagcuccu      540

gcauauagua uaagaugcau aggaguaagc aacagagauu uuguagaagg uaugucaggu      600

ggaacauggg uugauguagu acuugaacac gguggaugug ugacuguaau ggcacaggau      660

aaacccacag uggacauaga auuaguaaca acaacugugu caaauauggc ugaaguaaga      720

ucauacugcu augaagcauc aauuagugau auggcauccg auaguagaug uccuacacaa      780

ggagaagcau aucuagauaa acaaagugac acacaauaug ucuguaaaag aacguuaguu      840

gauagaggau ggggaaaugg augcggauua uuuggaaaag gaucacuugu aaccugugca      900

aaauuugcau guucaaagaa aaugacaggc aaaaguauac aaccagaaaa ucuagaauau      960

agaauaaugu uaucggucca uggaucacaa cauagcggua ugauuguaaa ugauacagga     1020

cacgaaacag acgaaaauag ggcaaaaguu gaaaucaccc caaacucacc cagagcagaa     1080

gcaacucugg gaggauuugg cagccuggga uuagauugug aaccaagaac uggacuugau     1140

uuuagugauc uauacuaucu uacaaugaac aauaaacauu ggcucguaca uaaagaaugg     1200

uuccaugaua uaccauuacc uuggcaugca ggagccgaua caggaacacc acacuggaac     1260

aauaaagaag cauuaguaga guucaaagac gcacaugcaa aaaggcaaac aguaguugua     1320

cuaggaagcc aagaaggagc aguucauacu gcauuagcgg gugcauuaga agccgaaaug     1380

gauggagcga aagguagauu aucuucugga cacuugaaau gucgcuuaaa aauggauaaa     1440

cuaagacuaa aaggaguaag uuacucauua ugcacagcag cauucacauu uacaaaaauu     1500

ccugcggaaa cacuacacgg aacuguaaca guagaaguac aauaugcugg aacugaugga     1560

ccauguaaag uucccgcaca aauggcaguu gacaugcaaa cauuaacacc gguaggaaga     1620

uugauuacug cgaacccugu uauaacagaa aguacggaaa auaguaaaau gaugcuagaa     1680

uuagauccac cuuuuggaga uaguuauaua guaauaggag uaggugaaaa gaaaauaaca     1740

caucauuggc acagaagcgg aucaacaaua ggaaaagcau uugaagcuac aguuagagga     1800

gcuaaacgaa uggccguacu uggagacaca gccugggauu uuggcuccgu aggaggagca     1860

uuaaacaguc uagguaaagg cauacaucaa auauuuggag cagccuuuaa aaguuuauuu     1920

ggagguaugu caugguuuuc acaaauacua auaggaacau uauuaaugug gcuaggacua     1980

aacacuaaaa auggaucgau aagccuuaug ugcuuagcac uaggaggugu uuuaauauuu     2040

cuaagcacag cagugucagc u                                               2061


<210>  2652
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short CDS opt

<400>  2652
acaucugugg gaauaguagg uuuauuauua acuaccgcaa uggcugccga aguaacaaga       60

agaggaucag cauauuauau guaccuugau agaaaugaug caggagaagc aauaucuuuu      120

ccaacaacau uagguaugaa uaaaugcuac auacaaauua uggauuuagg acauaugugu      180

gaugcaacga ugucauauga auguccaaug cuugaugaag gaguugaacc ugacgaugua      240

gauuguuggu guaauacaac aagcaccugg guuguauaug gaacauguca ucauaaaaaa      300

ggagaagcuc gaagaaguag aagagcagua acccuaccga gccacagcac uagaaaauua      360

caaacuaggu cacaaaccug gcuagaaagu agagaauaca caaaacaucu uauaagagua      420

gaaaauugga ucuuuagaaa uccagguuuc gcacuagcag cagcagcaau agcauggcua      480

cuagguucgu ccacaucaaa aaaaguaaua uauuuaguaa ugauauuacu aauagcacca      540

gcauauucaa uaagauguau agguguaagu aauagagauu ucguugaagg aaugagugga      600

ggaacauggg uagauguagu cuuagaacac ggaggaugug ugacuguuau ggcacaagac      660

aaaccaacag uagacauaga acuuguaacg acaacaguaa gcaauauggc agaaguacgu      720

aguuauugcu augaagcauc aauuucagau auggcaucag auuccagaug uccaacacaa      780

ggagaagcuu auuuagauaa acagucagau acgcaauaug uuugcaaacg aacccuaguu      840

gacagagggu ggggcaacgg augcggauug uucggaaaag gaucguuagu aacuugugcc      900

aaauucgcau guucuaaaaa aaugaccgga aaaucaauac aaccagaaaa uuuagaauau      960

agaaucaugc uaagcgugca uggcucacaa cacucuggca ugauuguaaa ugauacagga     1020

caugaaacug acgagaauag agcuaaagua gaaauaacac cgaauucacc cagagcagaa     1080

gcaacauuag gaggauucgg aucacuggga uuagauugcg aaccacgaac aggauuagau     1140

uucagugacc uuuauuauuu aacaaugaau aauaaacauu gguuaguaca uaaagaaugg     1200

uuccacgaca uaccauuacc uuggcaugca ggcgccgaua caggaacacc acauuggaau     1260

aauaaagaag cucuaguaga auuuaaagau gcacaugcaa aaaggcaaac aguagucguu     1320

cuaggaucac aagaaggagc aguacauaca gcauuagcag gagccuuaga agccgaaaug     1380

gauggcgcaa aaggaagacu aagcagcgga cauuugaaau gucgguuaaa gauggacaag     1440

uuaagguuaa aaggcguuag cuacaguuua uguacagcag cauuuaccuu cacaaaaauc     1500

ccugccgaaa cauuacaugg aacaguuaca guagaaguuc aauaugccgg gacugacgga     1560

ccauguaaag uaccagcuca aauggcagua gacaugcaaa cauuaacacc uguuggaaga     1620

cuaauaacag caaauccagu gauaacugag aguacagaaa acaguaaaau gauguuagaa     1680

uuggacccuc cauucgguga cagcuauauc guaauaggag uaggugaaaa aaaaauaaca     1740

caucacuggc acagaucagg aucaacaaua gguaaagcau ucgaagcaac aguacgugga     1800

gcaaaacgaa uggcgguucu aggagauacu gcgugggauu ucggaagcgu cggaggggca     1860

uuaaacucau uagggaaagg aauucaucaa auauuuggug ccgcauuuaa aagccuauuu     1920

ggagguauga guugguuuag ucaaauauua auuggaacac uccuuaugug gcucggauua     1980

aauacuaaaa acggaucuau uaguuuaaug uguuuggcau uagguggugu acuuauauuu     2040

uuaaguacag caguaucagc a                                               2061


<210>  2653
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short CDS opt

<400>  2653
acgaguguag guauaguagg auuauuacua accacagcua uggccgcaga aguaaccaga       60

agaggaagug cauacuauau guacuuagau cggaaugaug caggggaagc cauaagcuuu      120

ccaacaacuc uagguaugaa uaaaugcuau auacaaauaa uggacuuagg acauaugugu      180

gaugcgacaa ugagcuauga auguccaaug cuagacgaag gcguagaacc agaugauguu      240

gacugcuggu guaacacaac aaguacaugg guaguauacg ggacauguca ccauaaaaaa      300

ggcgaagcgc gaagaucaag aagagcaguu acauuaccaa gucauuccac uagaaaauua      360

caaacccguu cacaaacaug gcuugaauca cgagaauaca caaaacaucu uauucgugua      420

gaaaacugga uauuccgaaa uccgggauuu gcacuagcag cagcagcaau cgcaugguua      480

uuaggaaguu cuacaagaca aaaaguuaua uaccucguaa ugauacuuuu aauagcacca      540

gccuauagca uaagauguau aggaguaucc aacagagauu ucguugaagg aaugagugga      600

ggaacauggg uagacguagu cuuagaacau ggaggaugug uaacaguaau ggcucaagau      660

aaaccuacag uugauauaga acucguaaca acuacaguaa guaauauggc cgaaguccgu      720

ucauauuguu augaagcauc aauaucagau auggcgagcg acaguagaug uccuacccaa      780

ggagaagcau auuuagacaa acaaagugau acacaauaug ucugcaaaag aacauuagug      840

gacagagguu gggguaaugg augcggacua uucggaaaag gaaguuuagu aacaugugcc      900

aaauuugccu guucaaagaa aaugacagga aaaucaauac aaccagaaaa cuuagaauau      960

agaauuaugu uaucagucca uggcucacaa cacaguggga ugauagucaa cgacacagga     1020

caugaaacag augaaaauag agcuaaagua gaaauuacac caaauucacc ccgagcggaa     1080

gcaacacuag gaggauuugg aucacuagga uuagauugug aaccuagaac agguuuagac     1140

uuuagugauu uauacuaucu uacaaugaac aauaaacacu gguuaguaca caaagagugg     1200

uuccaugaca uaccacuucc auggcaugca ggagcagaca cggggacgcc acauuggaau     1260

aauaaagaag cauuaguaga auuuaaagau gcacaugcga aaagacaaac gguaguagua     1320

cuagguagcc aagaaggagc aguacacaca gccuuagcag gugcauuaga agcagaaaug     1380

gacggagcua aaggacgguu aucaaguggg cacuuaaaau gcagacuaaa aauggacaaa     1440

uuaagauuaa aaggaguaag cuacucauua ugcacagcag cauuuacauu cacuaaaauc     1500

cccgcagaga cuuuacaugg cacagugacc guagaaguac aauacgcagg cacagaugga     1560

ccauguaaag uuccugcaca aauggcugua gauaugcaaa cguuaacacc aguugguaga     1620

uuaauaacug caaauccugu aauuaccgag uccacggaaa auucaaaaau gaugcuagaa     1680

uuagacccac cauuuggaga uucuuauaua guaauaggag ucggagagaa gaaaauaacu     1740

caucauuggc auagaagcgg aaguacaaua ggaaaagcau uugaagcaac agucagagga     1800

gccaaaagaa uggcaguucu aggagacacc gcuugggauu uuggaucagu aggaggugcc     1860

uuaaauucuc uaggaaaagg aauacaucag auauuuggug cagcauuuaa aaguuuauuc     1920

gguggaaugu cgugguuuuc acagaucuua auaggaacgc ugcuaaugug gcuaggacua     1980

aauacaaaaa auggaucaau aucauuaaug ugucuagcau uagguggcgu acuaauauuu     2040

uuaucaacag caguaucagc a                                               2061


<210>  2654
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short CDS opt

<400>  2654
acaagugucg gcauaguagg ucucuuacug acaacagcga uggcagcaga aguaacaaga       60

agaggcagug cauacuacau guauuuagau aggaaugaug ccggggaagc aauaucauuc      120

ccaacaacuu uagguaugaa uaaauguuau auacaaauaa uggauuuggg gcauaugugu      180

gacgcuacca ugaguuacga auguccaaug cuugaugaag gugucgaacc agaugaugua      240

gauuguuggu guaauacaac uaguacaugg guaguuuaug gaacuuguca ucacaaaaaa      300

ggagaagcga gaagaagcag aagagcugug acacuccccu cccauaguac uagaaaacua      360

caaacacgaa gccaaacaug guuagaauca agagaauaua caaaacauuu aauaagagua      420

gaaaauugga uuuuuagaaa uccaggauuu gcauuagcug cagcugcaau agcauggcuc      480

cuggguucau caacuaguca gaaagugauu uauuuaguua ugauacuacu aaucgcaccc      540

gcguauucaa uaagguguau agguguaucc aacagagauu ucguagaagg aaugucugga      600

ggaacauggg uugauguagu uuuagaacac ggugggugug uuacaguaau ggcucaagac      660

aaaccaacug uagauauuga acuaguaaca acuacuguaa gcaauauggc agaagucaga      720

aguuauuguu acgaagccuc aauaagcgac auggcuucag auucuagaug uccaacacaa      780

ggggaagcau auuuagauaa acaaucagau acacaauaug ucugcaaaag aacacuaguc      840

gacagaggau gggggaacgg uugcggguua uucggcaaag gaucauuagu uaccugcgca      900

aaauucgcau guagcaaaaa aaugacgggu aaaaguauac aaccagaaaa ucuugaauau      960

agaauaaugc uaucuguuca cgguucacaa cauaguggaa ugauagucaa ugauacagga     1020

caugaaaccg augaaaacag agcaaaaguu gaaaucacac caaauucacc ucgagcagag     1080

gcuacauuag gaggcuuugg uagucuugga uuagacugug aaccgagaac aggauuagau     1140

uuuagugauu uauauuauuu aacaaugaau aauaaacauu ggcugguaca caaagaaugg     1200

uuucaugaua uuccacuucc auggcacgca ggugcagaua cagguacacc gcacuggaau     1260

aacaaagaag cauuagucga auuuaaagac gcacaugcaa aaagacaaac aguaguuguu     1320

uuaggcacuc aagaaggugc aguccauacg gcacuagcgg gggcacuaga agcagaaaug     1380

gauggugcua aaggaagacu aaguucagga cauuuaaaau guagauuaaa aauggauaaa     1440

uuaagacuaa aaggaguauc auauucauua uguacagcag cauuuacauu uacgaaaauu     1500

ccagccgaaa cccuacacgg uacuguuacu guagaagucc aauaugcagg uacagaugga     1560

ccauguaaag uaccugcgca aauggcagua gauaugcaaa cacuuacacc aguaggaaga     1620

uuaauaacug cgaauccagu aauaacggaa uccacagaaa auucaaagau gauguuagaa     1680

uuagauccac cuuuuggaga uucauacaua guaaucggag uuggugaaaa aaaaauaaca     1740

caucauuggc auagaucagg cucaacaaua ggaaaagccu uugaggcaac aguaagaggc     1800

gccaaaagaa uggcaguccu aggugacaca gcaugggauu uuggaucugu aggaggagca     1860

uuaaauuccc uaggaaaagg aauacaccaa auuuuuggag ccgcuuuuaa auccuuauuu     1920

ggaggaaugu caugguuuuc acaaauucua auuggaacac uauugaugug guuaggcuua     1980

aacacaaaaa acggaagcau caguuuaaug ugucuggcac ucggaggagu acuaauauuc     2040

cuaucaacag caguuagcgc a                                               2061


<210>  2655
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short CDS opt

<400>  2655
acuucuguag gaauaguagg ccuauuguua acaacugcca uggccgcaga agucacaaga       60

aggggcagcg cauacuauau guauuuagau agaaacgaug caggagaggc aauuucauuu      120

ccaacaacac uaggaaugaa uaaauguuau auacaaauaa uggaucuugg ucauaugugu      180

gacgcaacaa ugucguauga auguccuaug cuugacgaag guguugaacc agaugaugua      240

gauuguuggu gcaauacaac aucaacuugg guaguauacg gaaccuguca ucauaaaaaa      300

ggagaggcaa gaagaucaag aagagcaguu acgcuaccua gucauucgac aaggaaauua      360

caaacaagaa gucaaacaug gcuagaaagc agagaauaca cuaaacaccu aauaagagua      420

gaaaacugga uauuuagaaa uccuggauuu gcacuagcag cugcggcaau agcauggcua      480

uugggcucau caacaagcca aaaaguaaua uaucuaguca ugauacucuu aauagcacca      540

gcauacagcu uaagauguau aggagucagc aaucgagauu uuguagaggg uaugagugga      600

gguacauggg uggauguagu ucuugaacau ggagggugug uaacaguaau ggcucaagau      660

aaaccaacag uagauauaga guugguaacg acaacaguca gcaauauggc agaaguaaga      720

agcuacuguu acgaagcauc aauaucagau auggcaagug acucaagaug uccaacacaa      780

ggagaagcau auuuagauaa acaauccgau acucaauaug uauguaaacg aacguuagua      840

gacagagguu ggggaaaugg auguggccuu uuuggaaaag guucacuagu aacuugugcc      900

aaauuugcau guucaaaaaa aaugacagga aaaucaauac aaccagaaaa uuuagaauau      960

agaaucaugc uuucagucca uggaagucag cacucaggaa ugaucgucaa ugauacagga     1020

caugaaacug augaaaauag agcuaaaguu gaaauuacac cgaauucccc aagggcugaa     1080

gcaacucuug gcggauuugg uagucugggu cuagacugcg aaccaagaac agguuuagac     1140

uuuucagauu uauacuaccu aacaaugaau aauaaacauu gguuagugca uaaagaaugg     1200

uuucacgaua uuccauuacc auggcacgca ggugcagaua cagguacacc acauuggaau     1260

aauaaagagg cacuaguaga auuuaaagau gcacacgcaa aaagacaaac aguaguagua     1320

uuagguucac aagaaggagc aguacauaca gcuuuagcag gagcauuaga agccgaaaug     1380

gacggcgcca aaggaagacu uucaagcggg cauuuaaaau gcagauuaaa aauggauaaa     1440

cuuaggcuaa aaggaguaag uuauaguuua uguacagccg cuuucacauu uacuaaaaua     1500

ccagcagaaa cacuacacgg uacaguuacu guugaaguuc aauacgcagg uacagauggu     1560

cccuguaaag uaccagcaca aauggcugua gacaugcaaa cgcuuacccc aguaggaaga     1620

uuaauuacug caaacccagu aauaacagaa ucaacagaga acagcaaaau gauguuagaa     1680

uuagauccac cuuucgguga uucauauaua guaauaggag uaggagaaaa aaaaauaaca     1740

caucauuggc auagaagugg gaguacaauu gguaaagcgu ucgaagcuac uguuagagga     1800

gcaaaacgaa uggcgguauu aggagauaca gccugggauu ucggaagugu ugguggagca     1860

uuaaauaguu uaggcaaagg aauacaucaa auauucggag cugcauuuaa gagucuauuc     1920

ggugguauga guugguucuc acaaauacua auuggaacac uauuaaugug guuaggauua     1980

aauacaaaaa acggaaguau aucacuaaug uguuuagcau uagguggagu uuuaauuuuu     2040

cuaagcacag caguaucagc u                                               2061


<210>  2656
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short CDS opt

<400>  2656
acgaguguug gcauuguagg guuauuauua acaacggcua uggcagcaga aguaacaaga       60

agaggaucag cauauuauau guauuuagau agaaacgacg caggagaagc aauaucuuuc      120

ccaacaacac uagguaugaa uaaauguuau auacaaauaa uggaucuagg ucauaugugu      180

gaugcuacaa ugagcuauga augcccaaug cuagaugaag gaguagaacc ggaugacgua      240

gacuguuggu guaauacaac aucuacaugg guaguauacg gaacauguca ccauaagaaa      300

ggagaagcaa gaagaucgcg uagagcaguc acacuccccu cacauucaac cagaaaauua      360

caaacuagaa gucaaacuug guuagaauca agagaauaua cuaaacacuu aauaagaaua      420

gaaaacugga uauucagaaa uccaggauuu gcauuagcug cggcagcuau agcauggcuc      480

uuaggaucuu cuaccaguca aaaaguaaua uaccuaguaa ugauacucuu aauagcacca      540

gcauauucua uacgaugcau uggagugagu aacagagauu uuguugaagg aaugucagga      600

ggaacauggg uagacguugu ucucgaacac ggaggaugug uaaccgugau ggcucaggau      660

aaaccaacag uagauauuga auuaguaaca acaacaguuu caaauauggc agaaguccga      720

aguuacuguu acgaagcauc cauaucagac auggcaucgg auucaagaug uccaacccaa      780

ggagaagccu auuuagauaa acagucagac acucaauaug uuuguaaacg aaccuuagua      840

gaucgaggcu gggguaaugg auguggacuu uucgguaaag gcagucuugu aaccugcgca      900

aaauucgcau guaguaagaa aaugacagga aaaaguaucc aaccagaaaa ccuggaauau      960

agaauuaugc uaucagucca cggaucacaa cauucaggaa ugauaguaaa ugauacuggu     1020

caugaaacag augaaaauag ggcaaaagua gaaauaacuc caaauucacc acgagccgag     1080

gccacccuug gaggauuugg aucauuaggc cuagauugcg aaccuagaac aggauuagau     1140

uuuagugauu uauacuauuu aaccaugaac aacaaacauu gguuaguuca uaaagaaugg     1200

uuucaugaua uaccauuacc auggcaugcu ggagcagaua cagguacacc acauuggaac     1260

aauaaagaag cauuaguuga auuuaaagau gcacacgcua aacgucaaac uguuguuguu     1320

uuaggaucac aggaaggagc uguacauaca gcauuagcgg gugccuuaga agcagaaaug     1380

gacggagcaa aaggucgacu aaguaguggu cacuuaaaau guagauuaaa aauggacaaa     1440

uuaagauuaa aagguguuuc cuauuccuua uguacagcag cguuuaccuu caccaaaaua     1500

ccagcagaaa cccuacacgg aacaguaaca guagaaguac aauaugcagg uacagaugga     1560

cccuguaaag uuccugcaca aauggcagua gacaugcaaa cuuuaacccc aguaggaaga     1620

uuaauaacag ccaauccagu gauuacagaa uccacagaaa acucgaaaau gauguuagaa     1680

uuggauccac cauucgguga uaguuacaua guuauaggag uaggagaaaa gaaaauuaca     1740

caccauuggc acagaagcgg aagcacaaua ggaaaagcau ucgaagcaac aguaagagga     1800

gcaaaaagaa uggcugucuu aggagauaca gcaugggauu ucgguucugu uggcggagcu     1860

uugaacaguu ugggcaaagg aauacaucaa auauuugggg cagcauuuaa gagcuuauuu     1920

ggagguaugu caugguucuc acaaauauua auaggaacau uacuaaugug gcuaggauua     1980

aauacgaaaa auggcagcau uucacuaaug ugucuagcau uagguggagu cuuaauauuu     2040

cuaucaacag caguaucagc u                                               2061


<210>  2657
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short CDS opt

<400>  2657
acaucagugg gaauuguggg auuauuauua acaaccgcua uggcagcaga agucacuaga       60

agaggaucug cauauuacau guaucuagau agaaacgaug caggagaagc aauaaguuuc      120

ccaacaacau uagguaugaa uaaauguuac auacagauaa uggaucuagg ucauaugugc      180

gaugcaacua ugaguuauga augcccaaug uuagaugaag gaguagaacc agaugaugua      240

gauuguuggu guaacacaac aucaacaugg guagucuacg gaacauguca ucauaagaaa      300

ggugaagcua gaagaagccg cagggcagua acuuuaccgu cgcacucaac aagaaaacuu      360

cagacaagaa gucaaacuug guuagaaagu agagaauaua caaaacauuu aauccgugua      420

gaaaauugga uauuaaggaa cccaggauuc gcauuagcag cagcggcaau agccugguua      480

cuagguucaa gcacgucaca aaaaguaaua uacuuaguca ugauacuguu aauugcacca      540

gcauauagca uucguuguau uggcguaucu aacagagauu ucguugaagg aaugucggga      600

ggaacauggg uugauguagu ucuagaacau ggcgguugug uaacaguaau ggcccaagau      660

aaaccaacag uagauaucga acucguaacu acaacgguau ccaauauggc agaaguucga      720

aguuauuguu acgaagcaag uauaagugac auggccuccg auucaagaug uccuacgcag      780

ggugaggcuu aucuagacaa acaaucggac acacaauaug uauguaaaag aacauuagua      840

gauagaggau ggggaaaugg auguggacua uuuggaaaag gaaguuuagu aaccugugca      900

aaauuugcuu gcaguaagaa aaugacaggu aaaagcauac aaccagaaaa ucuugaauau      960

agaauaaugc ugagcguaca cggcagccaa cacucuggaa ugauuguaaa cgauacuggg     1020

caugaaacag augaaaaucg agcaaaagua gaaauaacac caaacagccc aagggcugaa     1080

gcaacccugg guggauuugg aaguuugggu cuagauugcg aaccaagaac cggucuagau     1140

uucucagauu uauauuauuu aacgaugaau aauaaacauu gguuaguaca uaaagaaugg     1200

uuccaugaua uaccauuacc auggcaugca ggagcugaua caggaacacc acauuggaau     1260

aacaaagaag cauuaguaga guucaaagau gcacacgcaa aaagacaaac uguaguagua     1320

cuaggaaguc aagaaggagc aguccacaca gcauuagcag gagcucuaga agcagaaaug     1380

gauggugcaa aaggccgacu aucuucugga cauuuaaaau guagauuaaa aauggacaaa     1440

cuaagauuaa aaggaguguc auacucauua ugcacagcag cauuuacauu uacaaaaauu     1500

ccagcagaaa cacuacaugg aacaguaacu guagaagucc aauaugccgg aacagacgga     1560

ccuuguaaag uaccagcaca aauggccgua gauaugcaaa cauuaacacc uguagguaga     1620

cuaauuacag ccaauccagu aauaacugaa uccacugaaa auagcaaaau gaugcuagaa     1680

cuggauccuc cauuuggaga uucauauaua gucauaggga uaggagagaa aaagauaaca     1740

caccacuggc auagaucugg aaguacuaua gguaaagcau ucgaagcaac aguaagaggu     1800

gcaaaaagga uggcaguauu aggagacaca gccugggauu ucggaucagu aggaggugca     1860

uuaaauucau uaggaaaagg uauccaccaa auuuucggag cagcuuuuaa aucuuuauuc     1920

gguggaauga guugguuuuc acaaauauua auuggaacuu uacuaaugug gcuaggauua     1980

aauaccaaaa auggaucaau aagucuuaug ugucuagcau uagguggagu acuuauauuu     2040

cuaaguacag caguaucagc u                                               2061


<210>  2658
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short CDS opt

<400>  2658
acuucaguag gaaucgucgg acuuuuguua acaacggcca uggcugcaga gguaacaaga       60

agaggaucag cuuauuacau guauuuagau aggaaugaug cuggagaagc cauaaguuuc      120

ccaacuacac uaggaaugaa uaaauguuau auucaaauaa uggauuuagg ucauaugugu      180

gacgccacua ugaguuacga guguccaaug uuggaugaag gcguagaacc agacgauguu      240

gacuguuggu gcaacaccac aucaacaugg guaguauaug guacauguca ucauaagaaa      300

ggagaagcaa gaagaucaag aagggcagua acacucccuu cacauucaac aagaaaauua      360

caaacgagaa gccagacaug gcuagaguca agagaauaua cuaaacacuu aauaagagua      420

gaaaauugga uauucagaaa uccaggguuu gcauuagcag cagcagcaau agcuugguua      480

cuaggaagua guacgucaca aaaaguaaua uaucuuguaa ugauuuuauu aauugcacca      540

gcuuauagca uuagauguau aggcguuucc aaccgugauu uuguugaagg aaugucugga      600

ggaacauggg uugauguagu gcuagaacac ggaggaugug uaacaguuau ggcacaagau      660

aaaccaacag uggacauaga gcuaguauca acuacuguca gcaauauggc ggaaguaaga      720

ucauacuguu acgaagccac aauaagugau auagcaucag acucaagaug cccaacacaa      780

ggagaagcau aucuagacaa acaaucagac acucaauaug uauguaaaag aacauuggua      840

gauagaggau ggggaaaugg augcggauua uuuggaaaag gcaguuuagu uacaugugca      900

aaauuugccu gcaguaagaa aaugacugga aaaucaauac aaccugaaaa uuuagaauau      960

agaauaaugu uaucaguaca ugggucucag cacucaggaa ugauagucaa ugacacagga     1020

cacgaaacug augaaaauag agcuaaaguu gaaauaacac caaauagccc acgagcagaa     1080

gcaacauuag gaggcuuugg aaguuuagga cuagauugug aaccaagaac aggccuagac     1140

uuuucagauc uauauuauuu aacaaugaac aacaaacauu ggcuaguaca caaagaaugg     1200

uuccacgaua uaccauugcc auggcacgca ggagcagaca caggaacucc acauuggaau     1260

aauaaagaag cucuuguaga auuuaaagau gcacaugcaa aaagacaaac agccguagua     1320

cuagguucac aagaaggagc uguacacaca gcacuagcug gagcauuaga ggcagaaaug     1380

gauggagcua aaggaagauu gucgaguggg caucuaaaau gcagauuaaa aauggacaaa     1440

uuacgauuaa aaggaguauc uuauucacua ugcaccgccg cauuuacauu uacaaaaaua     1500

ccggcagaaa ccuuacaugg aaccguaaca guugaaguac aauaugcagg aacagauggu     1560

ccauguaaag uaccagcuca aauggcaguu gauaugcaaa cauuaacacc agucggaaga     1620

cuuauaacag caaauccagu aauaacagaa uccacagaaa auagcaaaau gaugcuggaa     1680

cuagauccuc cauuugggga cucauacauc guaauaggag uaggagaaaa gaaaauaacg     1740

caccauuggc auagaagugg uaguacaaua ggaaaagcau ucgaagcuac aguaagagga     1800

gcgaaaagaa uggcaguauu aggugacacu gcuugggauu uuggaucagu cggcggggca     1860

uuaaauucau uagguaaagg aauacaucag auuuuuggag cagccuuuaa aucauuauuu     1920

ggugggauga guugguuuuc acaaauauua auaggaaccu uacuuaugug guuaggcuua     1980

aauacuaaaa augguagcau aucacuuaug ugcuuagcau uagguggagu cuuaauauuu     2040

uuaucuacag cagucagcgc c                                               2061


<210>  2659
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short CDS opt

<400>  2659
acaagcguag guauaguugg uuuacuuuua acaacagcaa uggcagcaga aguaacuaga       60

agaggaagug cauauuauau guaccuagau cguaacgacg cgggugaagc aaucagcuuu      120

cccaccacuu uaggaaugaa uaaauguuac auacaaauaa uggacuuagg acauaugugu      180

gaugcaacaa ugaguuauga auguccaaug cuagaugagg gaguugaacc agaugauguc      240

gacuguuggu guaauacaac aaguacuugg guuguauaug ggacuuguca ucauaagaaa      300

ggugaagcua ggagaucaag acgcgcagug acacuaccua gucauagcac uagaaaacuc      360

caaacacgau cacaaacaug guuagaauca cgcgaauaca caaaacacuu gauacgugua      420

gaaaacugga uauuucguaa cccagguuuc gcacuggcag cagcagcaau agcauggcua      480

uuaggaagua gcacuucaca aaaagucaua uaucuaguca ugaucuuauu aauagcacca      540

gcauauagua uaagaugcau agggguauca aacagagauu ucguagaagg aaugagugga      600

ggaacauggg uagacguagu acuggaacac ggaggaugcg uaacuguaau ggcacaagac      660

aaaccuacag uagauauaga guuaguaacg acuacaguua guaacauggc agaaguuaga      720

aguuauuguu augaagcaag uauaagugau auggcaagcg auaguagaug cccaacccaa      780

ggagaagcau auuuagauaa acagagcgac acucaauaug uuuguaaaag aacuuuagua      840

gacaggggau gggggaacgg auguggacua uuuggaaaag gauccuuggu uacaugugca      900

aaauuugcau guucaaagaa aaugacagga aaaucaaucc agccugaaaa ccuugaauac      960

agaaucaugc uaagcguaca ugggucacaa cacucaggaa ugauaguaaa cgauacggga     1020

caugaaacag augaaaacag agcaaaagug gaaauaaccc caaauagccc acgagcagaa     1080

gcaacucuug gagguuuugg aucucuagga uuagauugug aaccccgaac uggucuagau     1140

uuuagugacu uauauuaucu cacuaugaau aacaaacacu gguuaguaca uaaagaaugg     1200

uuccaugaua uaccacuacc cuggcaugcu ggagcagaca cgggaacacc acauuggaau     1260

aauaaagaag cucuaguaga auuuaaagau gcacacgcaa aaagacagac aguaguggua     1320

uuaggaucac aagaaggugc aguacauaca gcacuugcag gagcccuaga agcagaaaug     1380

gacggagcaa aaggccgacu aaguagugga caucuaaaau guagauuaaa aauggacaaa     1440

uuaagacuaa aaggaguauc uuauucauua uguacagcgg cauuuacauu uacuaaaaua     1500

cccgcugaaa cacuacacgg aaccguaaca guagaacuac aauaugcagg uacagauggu     1560

ccauguaaag uaccugccca aauggcugua gauaugcaaa cguuaacacc aguuggaagg     1620

uuaauaaccg caaacccagu aauaacagaa ucaacggaaa acagcaaaau gauguuagaa     1680

uuagacccac cauuugguga cucauauaua guaaucggag uaggagaaaa gaaaauaaca     1740

caucacuggc auagaagugg aaguacuaua ggaaaagcau uugaagcaac aguaagagga     1800

gcaaaaagaa uggccguauu aggagauacu gcuugggauu uugguucagu agguggagca     1860

cuaaacaguc uaggaaaagg aauacaccaa auauuuggug cagcauuuaa aagucuauuc     1920

ggcggaaugu cgugguuuuc acaaauacua auagguacac uauuaaugug gcuuggacuu     1980

aauacuaaaa auggaagcau cagcuuaaug uguuuagcau uaggaggcgu ccuaauauuc     2040

uuaaguacag caguaagugc u                                               2061


<210>  2660
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short CDS opt

<400>  2660
acaagcguag gcauaguagg auuacuauua acaacagcaa uggcugcaga aguaacaagg       60

cgaggaagug cauauuauau guauuuagau agaaaugacg caggcgaagc aauaaguuuu      120

ccaacaacau uaggaaugaa caaauguuac auacaaauaa uggauuuagg acauaugugu      180

gacgcaacaa ugucauauga auguccaaug uuagaugaag gaguggaacc agaugaugua      240

gacuguuggu gcaauacuac aaguacgugg guaguauacg gaacgugcca ucauaaaaaa      300

ggagaagccc gaagaagccg aagagcugua acucuaccca gccauucuac ccgcaaauua      360

caaaccagau cacaaaccug guuagaauca agagaauaua caaaacacuu aauuagaguu      420

gagaauugga ucuuuagaaa ucccgguuuu gcauuagcag cagcggcaau cgcauggcua      480

uuaggaucuu caacaucaca aaaaguaaua uacuuaguaa ugauauuauu aauagcucca      540

gcauacagua uaagauguau aggaguauca aauagagauu ucguugaagg aaugucugga      600

ggaacauggg uagauguagu acuagaacac ggcggaugug uuacaguaau ggcacaagau      660

aaaccuacag uagauauaga gcucgucaca acuaccguuu caaauauggc agaaguaagg      720

ucauauugcu acgaagcaag caucagugau auggcaagcg auucaagaug uccaacucaa      780

ggagaagcau aucuagacaa acaaucagac acacaauaug uaugcaaaag aacacuugua      840

gacaggggau gggguaaugg gugcggccua uucgguaaag guucgcuagu aacaugcgcg      900

aaauuugcau gcaguaaaaa aaugacaggu aaaaguauuc aaccagaaaa cuuagaauau      960

aggauaaugc uaucuguuca uggaucacaa cacagcggaa ugauaguaaa ugauacugga     1020

cacgaaacag augaaaaccg agcaaaagua gaaauaacac caaauagccc aagagcugaa     1080

gcaacucuag guggauuugg uucacuaggu uuggacugug aaccaagaac aggacuggau     1140

uuuucagauc uauauuauuu gaccaugaac aacaaacauu ggcuuguaca uaaagaaugg     1200

uuccaugaua ucccauuacc auggcaugca ggcgcggaua caggaacacc acauuggaau     1260

aacaaagaag cacuagugga guucaaagac gcacacgcaa aaagacagac aguaguagua     1320

cuaggaucac aagaaggagc aguucauaca gcuuuagccg gcgcuuugga agcagaaaug     1380

gauggagcaa aagguagacu uaguuccggc caucuuaaau gucgacugaa aauggauaaa     1440

cuacgacuaa aaggaguguc auauucacuc uguacagcag cauuuacuuu cacuaaaaua     1500

ccagcagaaa cauuacaugg aacuguaaca guagagguac aauaugcugg cacagacggc     1560

ccauguaaag uaccagcaca aauggcuguc gauaugcaga ccuuaacucc uguugguaga     1620

cuaauaaccg caaauccagu aauuacagaa aguauagaaa auagcaaaau gaugcuugaa     1680

cuagauccac cauuugguga uucguauaua guuauagggg uuggagaaaa aaaaauaacu     1740

caucauuggc auagaucagg aucaacuaua ggaaaagcau uugaagcuac aguaagagga     1800

gcaaaaagga uggcaguuuu aggugacaca gcaugggauu uugguucugu aggaggagca     1860

uuaaacuccc uagguaaagg aauacaucaa auauuuggug cagccuuuaa aagccuauuu     1920

ggagguaugu cuugguuuuc acaaauauua auaggaacac uucuaaugug gcuaggacua     1980

aacacuaaga auggaaguau cagcuuaaug uguuuagcac ucggaggagu acuaauauuu     2040

uuaucaacag caguguccgc a                                               2061


<210>  2661
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short CDS opt

<400>  2661
acaucaguug gaauaguagg ucuauuacua acaacagcaa uggcugcaga aguaacucgg       60

cguggaagcg cuuauuacau guacuuagac agaaaugaug caggcgaagc aauaucauuu      120

ccaacaacac uaggcaugaa uaaaugcuac auacaaauaa uggauuuagg gcauaugugu      180

gaugcaacua ugucuuacga auguccaaug uuagaugaag gcguagaacc agaugaugua      240

gauuguuggu gcaauacaac uucaacaugg guaguauacg guacauguca ucacaagaaa      300

ggagaagcua gaagaucaag gagagcggua acacuaccua gucauaguac aagaaaacua      360

caaacucgau cacaaaccug guuagaauca cgugaauaca caaaacauuu aauaagggua      420

gaaaauugga uauucagaaa uccagguuuu gcacuggcag cagcugcaau agcauggcuu      480

uuaggaucaa gcacaaguca aaaagucaua uaccugguga ugauauuauu aauagcucca      540

gcuuauagua uacgauguau aggaguaagu aauagagauu ucguagaagg aaugucagga      600

gguacauggg ucgaugucgu ucuagaacau ggaggaugug ucacgguaau ggcacaagac      660

aaaccaacag uagauauuga auugguaacu acaacuguaa gcaacauggc agaaguaaga      720

ucauauuguu acgaagcauc uauaagugac auggcaucag auucacgaug cccaacacaa      780

ggugaagcau auuuagauaa acaaucugac acucaauaug uauguaaaag aacucuagua      840

gaucgaggau ggggaaaugg uugugggcua uuuggaaaag gaagccuagu aacaugcgca      900

aaauuugccu guucaaagaa aaugacagga aaaucaaucc aaccagaaaa ccucgaauau      960

agaauaaugc uaucaguaca cggaucacaa cacagcggaa ugauaguaaa ugacacagga     1020

caugaaacag acgaaaacag agcaaaagua gaaauaacac ccaauagccc cagagcugaa     1080

gcaacauuag gaggauucgg aaguuuagga cuugacugug aaccaagaac aggauuagac     1140

uucagcgacc uauauuauuu aacaaugaau aacaaacauu ggcuaguaca caaagaaugg     1200

uuucacgaua uaccuuuacc auggcacgca ggcgcugaca cugguacucc acacuggaau     1260

aacaaagaag cacuuguaga auuuaaagau gcgcaugcaa aaagacaaac aguaguggua     1320

cuaggcucuc aagaaggugc aguacauaca gcacuagcag gcgcauuaga agccgaaaug     1380

gauggcguaa aaggaagacu aucaucgggg cauuuaaaau gucgucuaaa aauggacaaa     1440

uuaagacuaa aaggaguauc guauucauua ugcaccgcag cauucacauu uacaaaaaua     1500

ccagcagaaa cauuacaugg aacaguaaca guagaaguac aauaugcagg uacagacgga     1560

ccauguaaag uuccagcaca aauggcagug gacaugcaaa cauuaacacc gguaggaaga     1620

uuaauaacug caaauccagu uaucacggaa aguacagaaa auuccaaaau gauguuagag     1680

uuagacccuc cauuuggaga uaguuauaua guuauuggug uuggggaaaa gaaaauaacu     1740

caucacuggc auagaucagg caguacgaua ggaaaagcau uugaagcuac aguacgaggc     1800

gccaaaagaa uggcaguauu aggagauaca gcaugggacu uugguagcgu agguggagcc     1860

cuaaauucau ugggcaaagg uauucaucaa auauuuggag cugcauuuaa aagccucuuc     1920

gguggaauga guugguuuuc ucaaauacua auaggaacau uauuaaugug guuaggacua     1980

aauacuaaaa augguucuau uagcuuuaug uguuuggcac uaggaggagu cuugauauuu     2040

cuaaguaccg caguaucagc a                                               2061


<210>  2662
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short CDS opt

<400>  2662
acaucuguug guaucguagg auuauuacuc acaacagcaa uggcggccga aguaacuaga       60

aggggaucag cauauuauau guaucuagau agaaacgaug caggagaagc cauaaguuuu      120

ccgacaacau uaggcaugaa caaaugcuau auacaaauaa uggauuuagg gcauaugugu      180

gaugcaacca ugagcuauga augcccaaug uuagacgaag gaguagaacc agacgacgua      240

gauuguuggu guaacacgac aaguacaugg gucguauacg gaacuuguca ucauaaaaaa      300

ggagaagcua ggcguucaag aagagcagua acauuaccau cacauucaac acgaaaacuu      360

caaacaagau cacaaacaug gcuagaauca agagaauaua cuaaacauuu aauaaggguu      420

gaaaacugga uauuuagaaa cccaggauuu gcccuagcug cagcagcaau cgcauggcua      480

cuaggaucaa guacaagcca aaaaguaaua uaucuaguaa ugauacuauu aauagcucca      540

gcauacucga uuagaugcau agguguauca aauagagacu ucguagaagg aauguccgga      600

ggaacauggg uagacguagu guuagaacau ggaggaugug uuacaguaau ggcacaggac      660

aaaccaaccg uugacauaga auuaguuaca acaacaguua guaacauggc ugagguacgu      720

ucauauuguu augaagcauc aauaucagac auggcaucag auucacgaug ucccacucaa      780

ggugaagcau acuuagauaa acaaagcgac acacaauacg uuuguaaacg aacacuagua      840

gauagaggau gggggaaugg auguggccua uuuggaaaag gaagccuagu aacaugugcu      900

aaauuugcau guaguaaaaa aaugacagga aaaagcauuc aaccggaaaa cuuagaauau      960

agaauuaugu uaucaguaca ugguucacaa cauucaggga ugauaguaaa ugauaccgga     1020

caugaaacug acgaaaacag agcaaaagua gaaauaacuc cuaauagucc aagagcagaa     1080

gccacauuag ggggauuugg gagucuagga cuagauugcg aaccuagaac aggauuagau     1140

uuuucagauc uauauuauuu aacaaugaac aauaaacacu ggcuuguuca uaaagaaugg     1200

uuucacgaca uaccacuacc auggcaugca ggagcagaua caggaacacc acauuggaac     1260

aauaaagaag cauuagugga auuuaaagau gcacaugcaa aaaggcaaac ugucguaguu     1320

cuagguuccc aagaaggugc uguacauacu gcacuagcag gggcacucga agcagaaaug     1380

gacggagcua aaggaaaacu aaguucaggg caccuaaaau gcagauuaaa aauggauaaa     1440

uuacguuuaa aaggaguauc auauucauug ugcacagcag cauuuacauu uacaaaaauu     1500

ccggccgaaa cguuacaugg caccguuacg guagaaguac aauacgcagg aacagauggg     1560

ccauguaaag uaccugccca aauggcaguu gauaugcaaa cacugacacc gguugggaga     1620

cuaauuacug cuaacccagu aauaacugaa ucaacagaaa auucaaaaau gauguuagaa     1680

uuagauccac cauucggcga uagcuauauu guaauaggag uaggugaaaa aaaaauaaca     1740

caccauuggc acaggagcgg uaguacaauc ggcaaagcau uugaagcaac uguuagagga     1800

gcuaaaagaa uggcaguguu gggcgauaca gcaugggauu uugggagugu aggaggggca     1860

cucaacucau uaggaaaagg uauacaucaa auauucggag cagcuuuuaa aucauuauuc     1920

ggaggaauga gcugguuuag ucaaauacuu auaggaacac uauuaaugug guuaggauua     1980

aauacaaaga acggaagcau aucauuaaug uguuuagcac uuggaggagu uuuaauauuu     2040

cuaaguacag cugucagugc g                                               2061


<210>  2663
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short CDS opt

<400>  2663
acaagcguag gaauuguagg acuacuguua acaacagcaa uggccgccga aguaacaaga       60

agaggcagcg cauauuauau guacuuagau agaaaagaug cuggagaagc aaucuccuuu      120

ccaacaacau uagguaugaa uaaauguuau auacaaauaa uggaucuagg acauaugugu      180

gacgcaacaa ugucuuauga auguccaaug uuagaugaag gaguagaacc cgaugaugua      240

gacugcuggu guaauacgac aucaacuugg gugguauacg gaacaugcca ccauaaaaaa      300

ggagaagcaa ggagaucacg uagagcaguu acacuuccuu cucauucaac uagaaaauua      360

caaacacgaa gccaaacuug guuagaaagc agagaauaua caaaacauuu aauaagagua      420

gaaaacugga uauucagaaa uccaggauuu gcacucgcag cagcagcaau agcauggcua      480

uuggggucaa gcacaucaca aaagguaaua uacuuaguua ugauauuauu aauugcacca      540

gccuauucua uaagauguau aggaguaucu aacagagauu uuguugaagg uaugucaggc      600

ggaacauggg uagauguggu ucuugaacau gguggaugug uaacaguaau ggcacaagau      660

aaaccaacag uagauauaga auuaguaaca acaacaguaa guaacauggc agagguaaga      720

aguuauuguu augaagcaag uaucucagau auggcaagcg acucuagaug cccaacacaa      780

ggagaagcau aucuagauaa acaaagugac acacaauaug uauguaaaag aacacuagua      840

gauagaggau ggggaaaugg auguggacuu uuuggaaaag guucguuagu uacuugcgca      900

aaauuugcau guucaaaaaa aaugacugga aaaaguauac aaccagaaaa cuuagaguau      960

agaauaaugu uaucaguaca cgggucucaa cauaguggaa ugauuguuaa ugacacugga     1020

caugagacag augaaaauag agcaaaagua gaaauaacuc caaacucacc ccgugcagaa     1080

gcgacauuag gaggauuugg uucauuagga uuagauugug aacccaggac gggacuagau     1140

uuuagugauc uauauuaucu aacuaugaau aauaaacauu gguuaguaca uaaagaaugg     1200

uuucacgaca ucccauuacc auggcacgca ggagcagaua caggaacacc ucauuggaau     1260

aauaaagaag cauuaguuga auuuaaagac gcucaugcaa aacgucaaac cguaguugua     1320

cuagguagcc aagaaggagc aguacauacu gcauuagcag gagcauuaga agcagaaaug     1380

gauggcgcga aagguagauu aagcaguggc cauuuaaaau guagacucaa aauggauaaa     1440

cucagauuaa aaggaguuuc auauagucua uguacagcag cauuuacauu uacaaaaaua     1500

ccagccgaaa cacuccaugg aaccguaaca guagaagugc aauacgcagg aacagaugga     1560

ccgugcaaag uaccagccca aauggcagua gauaugcaaa cauuaacacc uguaggaaga     1620

uuaauaacag caaauccagu aauuacagag ucaacagaaa acagcaaaau gauguuagaa     1680

cuagaucccc cauucggaga uaguuauauu guaauaggug uaggugaaaa aaagaucaca     1740

caucauuggc acagaucagg aucaacaauu gggaaagcuu ucgaagcaac agucagaggu     1800

gcaaaacgaa uggcaguacu aggagauaca gcgugggacu ucggaagugu agguggagca     1860

cuaaacucac uggggaaagg aauacaccaa auauucggag cagccuuuaa aucacuauuu     1920

gggggcaugu cuugguucuc ucaaauacua auuggaaccu uauuaaugug gcuaggacua     1980

aacacuaaaa auggaagcau caguuuaaug ugucuagcac uuggaggugu uuuaauauuc     2040

uuaucgacag ccguuuccgc a                                               2061


<210>  2664
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short CDS opt

<400>  2664
acaaguguag gaauuguagg acuauuauua acaacagcua uggcugcaga aguaacaaga       60

agaggcagug cauacuauau guauuuagau agaaaugacg cuggagaagc aauuagcuuc      120

ccaacaacau ugggaaugaa uaaaugcuac auacaaauaa uggauuuagg ccauaugugu      180

gaugcaacaa ugaguuauga augccccaug cuagacgaag gaguugaacc agaugaugua      240

gauugcuggu guaauacaac uucaacaugg guuguuuaug guacauguca ccauaaaaaa      300

ggagaagcaa ggcguucaag acgagccgua acacucccuu cgcacucaac ucgaaaauua      360

caaacccgaa gccaaacaug gcuagaauca agagaauaua caaaacaucu gauaagaguc      420

gaaaacugga uauuuagaaa uccaggauuu gcacuagcag cagcagcaau cgcauggcug      480

cuugggagcu caaccucuca aaaagugauc uaucuuguaa ugauacuguu gauagcccca      540

gcauacucaa uaagauguau agguguaagc aauagagauu ucguagaagg caugucagga      600

ggaacauggg uugauguagu auuagaacac ggagguugcg uuacagugau ggcacaagac      660

aaaccaacag uagacauaga acuaguaaca accacuguaa guaauauggc agaaguacga      720

ucuuauuguu augaagcuag uauuucagau auggcaucgg auucaaggug uccuacucaa      780

ggugaggcau accuagacaa gcaaucagac acucaauacg uaugcaaaag aacacuaguu      840

gauagaggau ggggaaaugg auguggauug uucggaaaag guucauuggu aacaugugca      900

aaauuugcgu gcucuaaaaa aaugacaggu aaaucuauac aaccggaaaa cuuagaguau      960

agaauaaugc uaaguguaca uggcucucaa cauucuggaa ugauuguaaa ugauacagga     1020

caugaaacag augaaaauag agcaaaagua gaaauaacgc caaauagucc acgagcggaa     1080

gcaacacuag gaggauuugg uaguuuagga uuagacugug aaccgagaac aggauuagau     1140

uucagcgacc uauauuauuu aacgaugaau aauaaacauu gguuaguaca uaaagaaugg     1200

uuucaugaca uuccccuccc auggcacgca ggggcagaca cagggacacc acauuggaac     1260

aauaaagaag cacuaguaga auuuaaagaa gcacacgcaa aaagacaaac aguuguagua     1320

cuaggaaguc aagaaggagc aguacauaca gcacuagcag gagcuuuaga agcagaaaug     1380

gauggagcaa agggaagacu aucuucugga cacuuaaaau guagauuaaa aauggauaaa     1440

cugagacuaa aagguguauc auauucguua uguacagcag ccuucacauu uacuaaaaua     1500

ccagcagaaa cucuacaugg aacgguuaca guagaaguac aauaugccgg aaccgauggc     1560

ccgugcaaag uaccagcaca aauggcagug gauaugcaaa cauuaacccc aguaggaaga     1620

uuaauaacag caaauccagu aauaacagaa ucaacagaaa auagcaaaau gauguuagaa     1680

uuagauccac cuuuuggaga uagcuacaua guaauaggag ucggagaaaa aaaaauaacu     1740

caccauuggc acagaagcgg aucaacaaua ggaaaagcau uugaggccac aguuagagga     1800

gcaaaaagaa uggcaguacu aggagacacu gccugggauu uuggaagcgu uggaggggca     1860

uuaaauaguc uugguaaagg gauucaucaa auauucgggg cagcauuuaa aucacuauuu     1920

ggaggaauga guugguucuc acaaauauug auaggaacau ugcuaaugug guuaggacua     1980

aauacaaaaa auggcagcau aagccucaug ugucuugcuc uaggaggagu ucuaauauuu     2040

uuaaguacug caguaagugc u                                               2061


<210>  2665
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short CDS opt

<400>  2665
acaaguguug gaauaguagg auuacuacua acuacugcaa uggcagcaga aguaacuaga       60

agaggcucag cuuauuacau guauuuagac aggaacgaug ccggcgaagc gauaaguuuu      120

ccuaccacac uaggaaugaa caaauguuac auacaaauaa uggauuuggg acauaugugu      180

gaugcuacga ugagcuauga guguccaaug cuagacgaag gaguugaacc ugaugacgua      240

gauuguuggu guaacacaac aagcacaugg guuguauaug gaacauguca ucacaaaaaa      300

ggagaagcua gaagaucaag aagagcaguc acauuaccaa gucauagcac aagaaaacua      360

caaacaagau cacaaacaug guuagaauca agagaauaua caaaacaccu uauaagaguu      420

gaaaauugga uuuuuagaaa uccaggauuu gcuuuagcag cugcagcaau agcaugguua      480

cucgguucuu caacaagcca aaaaguaauc uaucuaguaa ugauauugcu aauugcccca      540

gcguacucua uucgauguau uggcguauca aacagagauu ucgucgaagg aaugucagga      600

ggcacauggg uagacguugu acuagaacac ggagggugcg uaacaguaau ggcacaggac      660

aaacccacag uugauauaga auuaguaacc acaacgguau caaacauggc ggaaguuaga      720

ucuuauuguu augaggcaag uauaagugau auggcaucag auaguagaug uccaacccaa      780

ggugaagcau acuuagauaa acaaucagac acacaauacg uauguaaaag gacacuggua      840

gacagaggau ggggaaaugg auguggauua uuugguaaag gaaguuuagu aacaugugca      900

aaauuugcau guucaaaaaa aaugacggga aaaaguauac aaccagaaaa uuuagaauau      960

agaauuaugu uaaguguuca uggaagccaa cauagcggua ugauagugaa cgacacaggu     1020

cacgaaacug augaaaacag ggcgaaagua gaaauaacac caaauagucc gcgcgcagaa     1080

gcagcuuuag gaggauucgg aucacugggu uuagauugcg aaccaagaac agguuuagau     1140

uucagugacu uguacuaccu aacaaugaau aauaaacauu gguuagugca caaagagugg     1200

uuucaugaua uaccauugcc auggcaugcc ggagcagaua caggcacgcc acauuggaau     1260

aauaaagagg cguuaguuga guucaaagau gcacaugcca aacgacaaac aguaguagua     1320

uuaggaucac aagaaggggc aguucacaca gcauuagcag gagcauuaga agcagaaaug     1380

gacggagcua aaggaagauu aagcucagga caccuaaaau guagauuaaa aauggauaag     1440

uugcgauuaa aagguguauc uuacucacuu uguacagcag cuuucacauu uacaaaaaua     1500

cccgccgaaa cacuacacgg uacaguaaca guagagguac aauaugccgg aacagaugga     1560

ccauguaaag uuccagcaca gauggcagua gauaugcaaa cauuaacacc ugucggaaga     1620

uuaauaaccg caaauccagu gauaaccgaa ucuacugaaa auucaaaaau gauguuagaa     1680

uuagacccuc cauuuggaga uucauacaua guuauaggag uaggagagaa aaaaauuaca     1740

caucauuggc auagauccgg uucaacuaua ggaaaagcau uugaagcuac aguaagaggc     1800

gcaaaacgua uggcaguauu aggagacacg gcaugggauu ucggaucagu aggaggagca     1860

uuaaacucau uaggaaaagg aauacaccaa auuuucggug cggccuuuaa aucauuauuu     1920

gguggaauga gcugguuuuc gcaaacauua auaggcacuu uacucaugug guugggacuu     1980

aauacaaaaa auggaagcau aucacucaug uguuuagcac ugggaggagu acuuauauuu     2040

uuauccacug caguuucagc a                                               2061


<210>  2666
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short CDS opt

<400>  2666
acaaguguag guauuguagg acuuuuauua acuacagcaa uggcagcaga gguuacuaga       60

agaggaucug cguauuauau guaucuagac agaaaugacg caggagaagc aauaucuuuu      120

ccuacgacau uaggaaugaa uaaaugcuau auacaaauaa uggauuuagg ccacaugugc      180

gaugcaacca ugucauauga auguccaaug uuagaugaag gaguugaacc agaugaugua      240

gauugcuggu guaauacaac aucaaccugg guaguauaug gaaccuguca ccacaaaaaa      300

ggcgaagcaa gaagaaguag aagagcagua acauuaccau cacauagcac acgaaaacuu      360

caaacacgaa gccaaacaug gcuugaaagu agagaauaua cuaaacaucu aauaagagua      420

gaaaacugga uauucagaaa uccaggcuuu gcauuagcag cagcugccau ugcauggcua      480

uuaggcaguu caacaucaca aaaaguaauu uauuuaguaa ugauucuauu aauugcaccu      540

gcauauagua uaaggugcau aggaguuucc aauagagauu uuguagaagg aaugucagga      600

gguacauggg uagauguagu ucuagaacau ggugguugug uaacaguaau ggcucaggau      660

aaaccaacag uagauauaga acuaguaacg acaacaguaa gcaacauggc agaaguuaga      720

agcuauugcu acgaagcaag uauaucagau auggcaagcg auucacgaug uccaacacaa      780

ggagaagcuu accuugacaa acaaucugau acucaauaug uaugcaaaag gacguuagua      840

gaucgugguu ggggaaaugg uuguggcuua uucggaaaag gaagucuagu uacaugcgcc      900

aaauuugcau guaguaaaaa gaugacaggu aaaucaauac aaccagaaaa uuuagaauau      960

cgaaucaugu uaucaguaca ugguucacaa cauucuggua ugauuguaaa ugacacagga     1020

caugaaacag acgaaaauag agcaaaagua gaaaucacac caaacucucc aagagcugaa     1080

gcaacuuuag gaggguucgg cucguuagga uuagauugug aaccaagaac gggacuagac     1140

uuuucagauc uauauuauuu gacaaugaau aauaaacacu ggcuaguuca uaaagaaugg     1200

uuucaugaua uaccacuacc auggcaugcc ggagcagaca cuggaacccc ccauuggaau     1260

aacaaagaag cacuaguaga auuuaaagau gcucacgcga aacgacaaac uguaguagua     1320

cuuggaucac aagaaggagc aguacacaca gcauuagcag gagcuuuaga agcugagaug     1380

gacggcgcua aaggacgacu aaguucaggc cauuuaaaau gcagauuaaa aauggauaaa     1440

cuaagauuaa aaggaguuuc auauucacua uguacagcag cuuuuacauu uaccaaaaua     1500

ccugcagaaa cauugcaugg aacaguuaca guagaaguac aauacgcugg agcugaugga     1560

ccuuguaaag uacccgcuca aauggcagua gauaugcaga cauuaacacc agucggaaga     1620

uuaauuacag ccaauccagu aauuacagaa ucaacggaaa auucaaaaau gauguuagag     1680

cuagacccgc cauuuggaga uucauauauu gugaucggug uaggcgaaaa aaaaauuaca     1740

caccauuggc auagaucagg uucuacaaua ggcaaagcgu uugaagcaac ugugaggggc     1800

gcaaaaagaa uggcaguauu aggagauacu gcaugggauu ucggaagugu aggaggagca     1860

cuaaacuccc ugggaaaagg uauccaucaa auauuuggag cggcguucaa aucauuauuc     1920

gguggaaugu caugguucuc acaaauccuc auaggaacuu uauuaaugug guuaggacuu     1980

aauacaaaaa auggcaguau aucauuaaug ugccuagcac uaggaggagu cuuaauauuu     2040

uuaaguacug caguuucagc a                                               2061


<210>  2667
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short CDS opt

<400>  2667
acaucagugg gaauaguagg uuuacuacua acaacagcaa uggcagcaga aguaacaaga       60

agaggaucug cauauuauau guauuuggau agaaacgaug caggggaagc aaucaguuuu      120

ccuacaacgu ugggaaugaa uaaauguuau auacaaauaa uggaccuagg acauaugugu      180

gaugcuacaa ugucguauga augcccgaug cuggaugaag ggguagaacc agaugaugua      240

gacuguuggu guaauacaac caguacaugg gugguauaug gaacauguca ucauaaaaaa      300

ggugaggcuc gacgaaguag gagagccgua acauuaccuu cacauucaac aagaaaauua      360

caaacaaggu cacaaacaug guuagaaagu agagaauaua caaaacaucu aauuagaguu      420

gaaaacugga uauuuagaaa cccaggguuc gcuuuggcag cagcagcaau agcaugguua      480

uuaggaagcu caacaucuca aaaaguuaua uaucuaguca ugauauuacu uauagcauua      540

gcuuauucca uacgauguau cggaguauca aauagagauu ucguagaagg aaugucagga      600

ggaacauggg uugacguagu guuagaacau gguggcugug uaacaguaau ggcacaagau      660

aagccaacag uagacauuga auuaguaacu acaacaguaa guaauauggc agaaguaaga      720

ucuuauuguu acgaagcaag uaucagcgau auggccuccg auucaagaug cccgacacaa      780

ggugaagcau auuuagauaa acaaagcgau acgcaauacg uauguaaacg uaccuuggua      840

gacagagguu gggguaaugg augcggauua uuuggaaaag gcucacuugu uacgugugca      900

aaauucgcau gcucaaaaaa aaugacugga aaauccauac aaccagaaaa ucuagaauau      960

agaaucaugc uauccguaca uggaucccaa cauucaggua ugaucguaaa ugauacaggg     1020

caugaaacag augaaaauag agcgaaagua gaaauaaccc caaauagucc aagagcugag     1080

gcaacuuuag gaggauuugg aucacuaggc uuagacugcg aaccgaggac agguuuagau     1140

uuuagugauc uauauuaucu uacuaugaau aauaaacauu gguugguaca uaaagaaugg     1200

uuucacgaua uaccauuacc auggcaugca ggagcugaua caggaacacc acauuggaau     1260

aauaaagaag ccuugguuga auuuaaagac gcacaugcaa aacgacaaac aguaguggua     1320

uuaggaaguc aagaaggggc cgugcacaca gcauuagcug gagcucuaga agcagaaaug     1380

gauggagcaa aaggaagacu aucaucagga caccuaaaau gccgguuaaa aauggacaaa     1440

cuaagacuaa aaggggugag uuacucauug uguacugcag cauuuacauu cacaaagaua     1500

ccagcagaaa ccuuacaugg uacugucaca guagaaguac aauaugcagg aacagaugga     1560

ccauguaaag uaccugcaca aauggcagua gacaugcaga cauuaacacc aguagguaga     1620

cuaauaacug caaaucccgu uauaaccgaa ucaaccgaaa auaguaaaau gauguuagaa     1680

uuagauccac cuuuugggga uucauauaua gucauaggag uaggagaaaa aaaaaucacu     1740

caucauuggc acagaagcgg aucaacaauc gggaaagcau ucgaagccac aguuagagga     1800

gcaaaaagaa uggcagucuu aggagauacg gccugggauu uuggaagugu agggggggca     1860

uuaaauucau ugggaaaagg aauacaucaa auauuuggag cagcauuuaa aaguuuguuc     1920

gguggaaugu caugguuuuc ucaaauacua auaggaacuc uauuaaugug gcuaggucuu     1980

aauacaaaaa augggaguau aucauuaaug ugccuagcau uagguggagu cuugauauuc     2040

cuaagcacag caguaagcgc a                                               2061


<210>  2668
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short CDS opt

<400>  2668
acaaguguag gaauaguugg auuacucuua acaacagcaa uggcagcaga aguaacaaga       60

agggguucag cauauuauau guaucuagau cgaaaugaug caggagaagc aauaaguuuu      120

cccacaacuu ugggaaugaa caaauguuau auacaaauaa uggacuuggg acauaugugu      180

gaugcuacua uguccuauga auguccaaug uuagacgaag gaguagaacc cgacgauguu      240

gauugcuggu gcaacacaac aaguacuugg guuguauacg gaacaugcca ucacaagaaa      300

ggagaagcaa gaagaucaag acgagcagua acuuuaccaa gucauaguac aagaaaauua      360

caaacacgau cacagacaug gcucgaauca agggaauaua caaaacaucu aauuagagua      420

gaaaauugga uauucagaaa uccaggauuu gcauuagcag cugcagcaau cgcauggcuu      480

uuagguucau cgacuucaca aaaaguuaua uauuuaguaa ugauacuucu aauagccccc      540

gcauauucaa uucggugcau uggagucucg aaucgagacu uuguggaagg aaugucaggu      600

ggaacauggg uagauguagu cuuagaacau ggaggaugcg uuacuguaau ggcucaagau      660

aaaccuacag uagauauaga gcuaguaaca acuacaguaa guaauauggc agaaguaaga      720

aguuauuguu augaggcaag uauaucugau augguaucag auucaagaug uccaacacag      780

ggagaagcau accuagauaa acagucagau acucaauaug uuuguaaaag aacauuagua      840

gaucgaggau gggguaaugg cuguggauua uuuggaaaag gaucacuugu aacaugugcg      900

aaauuugcau guucaaagaa aaugacaggc aaaucaauuc aaccagaaaa uuuagaauac      960

agaauaaugc uaaguguaca cgguucacaa cauuccggca ugauaguaaa cgacacugga     1020

caugaaacug acgaaaauag agcaaaaguu gaaauaacac caaauagucc uagagcagaa     1080

gcaacauuag guggauuugg aucauuggga uuagauugug aacccagaac aggucuggau     1140

uuuucagauu uauauuaccu uaccaugaac aauaaacauu ggcuagugca uaaagaaugg     1200

uuccacgaca uaccacuacc uuggcaugca ggagcagaua caggaacacc acauuggaau     1260

aauaaagagg cuuuaguuga auuuaaagac gcacaugcua aaagacaaac aguaguagua     1320

uuaggaaguc aagaaggagc aguacauaca gcacuagcug gugcauuaga agcagaaaug     1380

gacggugcaa aagguagauu aucaucagga cacuuaaaau guagauuaaa aauggauaaa     1440

cuacgccuaa aagguguauc auauucucua uguacagcag cauuuacauu cacaaaaauu     1500

ccugcagaaa cauuacaugg uacaguaaca guagaaguac aguaugcagg aacggaugga     1560

cccuguaaag uaccagcaca aauggcagua gauaugcaaa cauuaacacc agucggaaga     1620

uuaauuacug ccaacccagu aauuacagaa aguacagaaa acagcaaaau gauguuagaa     1680

cuagacccac ccuuuggaga cucuuauaua guaauaggug uaggagaaaa gaaaauaaca     1740

caucauuggc aucguagugg uagcacaaua ggaaaagcgu ucgaagcaac aguaagagga     1800

gcaaaaagaa uggcuguauu aggugauaca gcuugggauu ucggauccgu aggaggagca     1860

cucaacaguu uaggaaaagg uauucaccaa aucuuuggag cagcauuuaa aaguuuauuu     1920

ggaggaauga gcugguuuag ucaaauauua auugguacuc uauuaaugug guuaggauua     1980

aauacuaaaa auggaucaau aagucuaaug ugccuagcau uaggcggugu acuaauauuu     2040

cugucuacag cgguuucagc u                                               2061


<210>  2669
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short CDS opt

<400>  2669
acaucaguug ggauuguggg ucuucuuuua acaacagcua uggcagcaga aguaacaaga       60

agaggaucag cauacuacau guauuuagau cgaaaugaug cuggagaagc aauaaguuuu      120

ccaacuacuu uaggaaugaa uaaauguuau auacaaauua uggauuuagg acauaugugu      180

gaugcuacua ugagcuauga auguccaaug cuagaugaag gaguagaacc cgacgaugua      240

gauugcuggu guaacacuac aagcacgugg guuguauaug gaacuugcca ccauaagaaa      300

ggagaagcua gaagaaguag acgagcagua acacuaccua gucauucaac gagaaaacua      360

caaaccagau cacaaacaug gcuagaauca agagaauaua caaaacaccu cauaagaguu      420

gaaaacugga uauuuaggaa cccaggauuu gcucuagcag cagcugcaau cgcaugguua      480

uuaggauccu caacaucaca aaaaguuaua uacuuaguaa ugauuuuauu aauagcacca      540

gcuuauucca uuagauguau agguauaagc aacagggacu uuguagaagg aaugagugga      600

ggaacuuggg ucgauguagu cuuagaacac ggcggaugcg uaacuguaau ggcacaggau      660

aaaccaacug uagauauaga auuaguaacc acaacuguau cuaauauggc ugaaguaaga      720

ucauacugcu augaagcaag uauaucugau auggcaucag acucaagaug cccaacacaa      780

ggagaagccu auuuagacaa acaaagugau acacaauacg uauguaaaag gacauuagua      840

gauagaggau ggggaaaugg cugcggauua uuugguaaag gaagucuagu aacuugugcc      900

aaauucgccu guucaaagaa aaugacagga aaaucaauuc aacccgaaaa ucucgaauau      960

cguauaaugu ugucaguaca cggaucacaa cauaguggaa ugauaguuaa cgacacuggc     1020

cacgaaacgg acgaaaaucg agcaaaagua gaaauaacac caaauagucc uagagcagaa     1080

gcgacauuag gaggauuugg cauauuagga uuagauugug aaccaagaac uggacucgau     1140

uuuucagauu uauacuauuu gacaaugaau aauaaacacu gguuaguaca caaagaaugg     1200

uuucaugaca uaccuuuacc guggcaugca ggagcagaca caggaacgcc ucacuggaac     1260

aauaaagaag cauuagugga guucaaagau gcacacgcaa aaagacaaac aguaguagua     1320

uuagguaguc aagaaggagc aguacauacc gcacuagcug gcgcuuuaga agcagaaaug     1380

gauggcgcaa aaggaagauu aucaagcgga cauuuaaaau guaggcuaaa aauggauaaa     1440

cuaagauuaa aaggaguaag cuauagcuua ugcacagcag cauucacauu uacaaaaaua     1500

ccagcagaga cauuacaugg uacaguuacu guagaagucc aauaugcagg uacugaugga     1560

ccauguaaag uaccggcaca aauggcagua gacaugcaaa cauuaacacc uguugguaga     1620

cuaaucacag caaauccugu aauaacagaa ucaacagaaa acucgaaaau gauguuagaa     1680

uuggacccuc cauuuggaga cucauauauu gucauaggag uaggagaaaa gaaaauaacu     1740

caucauuggc auagaucagg aucaacaaua ggaaaagcau uugaagcaac agugagagga     1800

gccaaaagaa uggcgguucu uggagacaca gcuugggauu uuggaucugu aggaggagca     1860

uuaaauaguu uaggaaaagg aauacaucaa auauuuggcg ccgcuuuuaa aagccuuuuu     1920

ggaggaaugu caugguuuag ucaaauacug auagguacgc uauuaaugug gcuugguuug     1980

aauacgaaaa auggaaguau aaguuuaaug ugucuggcac uuggaggggu auuaauauuc     2040

uuaucaacag cagugucagc a                                               2061


<210>  2670
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short CDS opt

<400>  2670
accaguguag ggauaguagg auuacuauua acuaccgcaa uggccgccga aguaacaaga       60

agaggcagug cauauuacau guacuuagac aggaaugacg ccggugaagc uauaaguuuu      120

ccaacaacuu uaggaaugaa uaagugcuau auucaaauaa uggacuuagg acauacgugu      180

gaugcaacaa ugucauauga auguccgaug uuggacgaag gaguagaacc agaugaugua      240

gauuguuggu guaacacaac gaguacaugg guuguauaug gaacuuguca ucacaagaaa      300

ggagaagcaa gaagaagcag acgagcagua acccuucccu cucacaguau aagaaaacua      360

caaacaagga gccaaacaug gcuagaaagu agagaauaua caaaacaucu gauuagaguu      420

gaaaauugga uauucagaaa uccuggauuu gcguuagcag cggcagcaau agcaugguua      480

uuagguaguu caacaaguca aaaaguaauu uaccuaguaa ugauauuacu aauugcacca      540

gcauacucua uaaggugcau cggaguauca aauagagauu ucguagaagg uaugucagga      600

ggaacauggg uagacguagu guuagaacau gguggaugug uaacuguaau ggcgcaagau      660

aaaccaacag uggauauaga acugguaaca acuacuguaa gcaauauggc agaaguacga      720

ucguauuguu augaggccag uauaucugau auggccagug acaguagaug cccaacacaa      780

ggugaagcau accuagacaa acaaucagac acucaguaug uuuguaaacg aacauuagua      840

gaucgagguu gggguaaugg auguggguua uucggaaaag gaaguuuagu aacuugugca      900

aaauuugcau guucaaagaa aaugacagga aaaucaauac aaccagaaaa ucuagaauau      960

agaauaaugu uaucuguaca ugguagccaa cacagcggaa ugauuguuaa cgacacagga     1020

caugaaacgg augaaaauag agcgaaaguu gaaauaaccc caaauagccc aagagcagaa     1080

gcaacuuuag guggauuugg aucauuaggu uuagauugug aaccaagaac cggacuagac     1140

uuuagugacc uauauuauuu aacaaugaau aauaaacauu gguuaguaca uaaagaaugg     1200

uuucacgaua uaccacuccc auggcacgcc ggagcagaua cagguacacc acacuggaau     1260

aauaaagaag cauuaguaga guucaaagau gcgcacgcaa aaagacaaac uguugugguu     1320

cuaggaaguc aagaaggcgc aguucauacu gcauuagcgg gagcacuaga ggccgaaaug     1380

gacggagcaa aaggaagacu uagcucaggu caucuaaaau gcagauuaaa aauggauaaa     1440

cuccgauuaa aaggaguauc auacaguuua uguacagcag cauuuacauu uacaaaaaua     1500

ccagcagaaa cucuacaugg aacugugaca guagaaguac aauacgcggg uacugacgga     1560

ccuuguaaag uaccagcuca aauggccgug gauaugcaaa cauuaacacc aguuggaaga     1620

uuaauuacag cuaacccagu aauaacugaa ucaacagaaa acagcaaaau gauguuagaa     1680

uuagacccgc cauuuggaga caguuauaua guaauuggag uaggcgaaaa gaaaauaacu     1740

caucacuggc accgaucagg aucaacaaua gguaaagcau uugaagcaac uguucgagga     1800

gcaaaaagga uggcaguacu aggcgauaca gcgugggauu uuggaucugu aggaggugcc     1860

uuaaauucuu uaggaaaagg uauacaccaa auuuuuggag cagcauuuaa aucacuauuu     1920

gguggaauga gcugguucuc acaaauauua auaggaacac uauuaaugug gcuuggucua     1980

aaugcuaaaa augguaguau aaguuuaaug uguuuagcau uaggaggagu acuaaucuuc     2040

cuaagcaccg caguaucagc a                                               2061


<210>  2671
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short CDS opt

<400>  2671
acauccguag guauaguagg auuacuacua acaacagcca uggccgcaga aguaacccgu       60

cgaggaucug cauacuauau guauuuagac cgaaacgacg caggagaagc uauauccuuc      120

ccaacaacuc uaggaaugaa uaaauguuau auucaaauaa uggauuuagg acauaugugc      180

gaugcaacca ugucauauga auguccaaug uuagaugaag ggguagaacc agaugaugua      240

gacugcuggu guaauacaac uuccacaugg guuguauacg gaacguguca ucauaagaaa      300

ggagaagcgc aaagaaguag acgagcaguu acacuaccaa gucauaguac aagaaaauua      360

caaaccagau cucaaacaug gcuagaauca agggaauaca cuaaacaucu aauaagagua      420

gaaaauugga uuuuuagaaa uccuggauuu gcacuagcgg cagccgcaau agcauggcua      480

uuaggaaguu cuacaucaca aaaaguaaua uacuuaguaa ugauacuauu aauagcacca      540

gcuuacagua ucagaugcau aggaguuuca aacagagauu ucguagaagg caugagugga      600

ggaacauggg uugauguagu acuagaacau gguggaugcg uaacaguaau ggcacaagac      660

aaaccaacag uagauauuga auuaguaaca acaaccguau caaauauggc ugaagucaga      720

aguuacuguu augaagcuag cauaagugau auggcaagug auucaaggug cccaacacaa      780

ggugaggcuu aucuagacaa acaaagugac acacaauaug uauguaaaag aacuuuagua      840

gaccgaggau ggggaaaugg auguggauua uucggaaaag gauccuuagu aacuugcgcu      900

aaauuugcgu guucaaagaa aaugacaggu aaaaguauac aaccagaaaa uuuagaauau      960

agaauaaugc uaucaguaca cggaagccaa cauucaggca ugauaguaaa cgauaccgga     1020

caugaaaccg acgaaaacag agcaaaaguu gaaauuacac ccaacagccc ccgagcagaa     1080

gcaacccuug gagguuuugg cagcuuagga uuagauugug aaccaagaac cggauuagau     1140

uuuagugacc uauauuaucu aacaaugaau aauaaacauu gguuaguaca caaagaaugg     1200

uuucacgaca uaccauuacc auggcaugca ggagcagaca cuggaacacc acauuggaau     1260

aauaaagaag cauuaguaga auuuaaagau gcucaugcaa aacgacaaac aguaguagua     1320

uuaggcaguc aagaaggggc cguacauaca gccuuagcag gggcacucga agcagaaaug     1380

gacggagcua aaggaagacu aucaaguggu cauuuaaaau gucgcuuaaa gauggauaaa     1440

uugagauuaa aaggaguaag uuauucacua uguacagcug cauuuacauu cacaaaaaua     1500

ccagcagaaa cacuucaugg aaccguaaca guagaaguac aauaugcagg gacagaugga     1560

ccauguaaag uaccugcaca aauggcaguu gauaugcaaa ccuuaacacc aguaggaaga     1620

cuaauaacag caaauccagu aauaacagaa ucgacagaaa auaguaaaau gauguuagaa     1680

uuagacccac ccuuuggaga cucauauaua gugauaggug uaggugaaaa gaaaauaacu     1740

caccauuggc auagauccgg aaguacaaua ggaaaagcau uugaagcaac aguaagagga     1800

gcgaaacgua uggcuguacu aggagauaca gcaugggauu uuggaucagu aggaggagca     1860

cuaaauagcc uuggaaaagg aauacaccag auauuuggag cagcauuuaa aucuuuauuu     1920

ggaggaaugu caugguucag ucaaauauua auagguacau uacuaaugug guuagguuua     1980

aacacuaaaa auggaucuau aucuuuaaug uguuuagcau uagggggagu uuuaauauuu     2040

cuaucaacag cuguaagugc a                                               2061


<210>  2672
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short CDS opt

<400>  2672
acuuccguag guauaguagg uuuacuauua acaacugcca uggccgcaga aguaacuaga       60

agagguagcg cauauuauau guacuuagau cgaaacgaug ccggagaagc aauaucauuc      120

ccaacaacau uaggcaugaa uaaauguuau auacaaauaa uggauuuagg acauaugugu      180

gaugcaacaa ugucauacga auguccgaug cuagacgaag guguagaacc agacgaugua      240

gacuguuggu guaacacaac uuccacaugg guuguuuaug gaacuuguca ucauaaaaaa      300

ggagaagcaa gacguucacg uagagcugua acccuaccau cacauucaac aagaaaacuu      360

caagcaagau cacaaacaug gcuagagucu agagaauaca caaaacauuu aauaagagua      420

gaaaacugga uauuuagaaa uccgggauuc gcuuuagcug cagcagcaau cgccuggcua      480

uuagguucaa gcacaaguca aaaaguaaua uacuuaguca ugauauuauu gauagcccca      540

gcuuauucaa uaagauguau aggaguuuca aacagggauu uugucgaagg aaugucagga      600

ggaacauggg uagaugucgu auuagaacac ggaggaugug uaacaguaau ggcacaagau      660

aaaccaacag uugacaucga auuaguuaca acgacaguaa gcaauauggc ugaaguaaga      720

ucuuauuguu augaagcaag cauuagugac auggcaagug auucaaggug cccaacacaa      780

ggagaagcuu auuuagacaa acaauccgau acucaauacg uauguaaaag aacauuagug      840

gauagaggau ggggcaaugg auguggccua uuugguaaag gaaguuuagu aacuugugca      900

aaauuugcau guaguaaaaa aaugacaggu aagagcauac aaccagaaaa ucuagaauau      960

cgaauaaugu uaucuguuca uggcagucaa cacucaggaa ugauaguaaa ugacacagga     1020

caugaaacug augagaauag agcaaaggua gaaauaacac caaacagccc aagagcugaa     1080

gcaacauuag gaggguuugg aucauuagga cucgauugug aaccaaggac uggcuuagac     1140

uuuucagauu uauauuauuu aacaaugaau aauaaacauu ggcuaguaca caaagaaugg     1200

uuucaugaua uuccgcuacc auggcacgca ggagcagaua cgggaacucc ccauuggaau     1260

aauaaagaag ccuuaguaga guucaaagac gcacacgcaa aaagacaaac agucguagua     1320

cuuggaaguc aagaaggagc aguacauaca gcuuuagcag gggcucuuga agcagagaug     1380

gacggagcaa aaggaagacu gucaagcgga caucuaaaau gcagauuaaa aauggauaaa     1440

cuaagauuaa aaggaguauc uuacagucuu ugcaccgcug cuuuuaccuu uacaaaaaua     1500

ccagcagaaa cuuuacaugg aacuguuacc guagaagucc aguacgcugg aacagauggc     1560

ccaugcaaag uacccgcaca gauggcugua gauaugcaaa cauuaacacc aguagguagg     1620

cugauaacag caaaucccgu aauaacagaa ucaacagaaa auucaaaaau gaugcuagaa     1680

cuugacccac cuuuuggcga uaguuauaua guuauaggag uaggagaaaa aaaaauaaca     1740

caucauuggc acagaucugg uuccacgauc ggaaaagccu uugaagcaac aguaagggga     1800

gcgaaaagaa uggcuguacu aggugacaca gccugggauu ucggaucagu uggaggugca     1860

cuaaauucau uaggaaaagg aauacaccaa auuuuuggag cagcauuuaa aucauuauuc     1920

ggcggaauga gcugguuuuc acagauuuua auaggaacac uauuaaugug gcuaggacua     1980

aauacaaaaa auggaucgau aagucuaaug ugucuagcac uaggaggagu auugauauuu     2040

cuaucaacgg caguuucagc a                                               2061


<210>  2673
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short CDS opt

<400>  2673
acaucaguag gcauaguagg acuauuacua acaacagcca uggcagcgga aguaacaagg       60

agaggaucug cauauuacau guacuuagau cgauccgaug caggagaagc aauaaguuuu      120

ccaacaacuu uagguaugaa uaaauguuau auacaaauaa uggauuuagg acacaugugu      180

gaugcuacua ugucuuauga augcccaaug uuagacgaag gaguggaacc ugaugauguu      240

gauuguuggu guaauacaac aaguacaugg guaguauaug guacauguca ucauaagaaa      300

ggagaagcga ggagaucaag gagagcugua acauuaccaa gucauagcac aagaaaauua      360

caaacaagaa gccaaacuug guuagaaagu agagaauaca caaagcauuu aauaagagua      420

gaaaauugga uauuuagaaa cccaggauuu gcacuagcag cagcagcaau agcauggcua      480

uuaggcagua guaccucaca aaaagucaua uauuuaguua ugauacuuuu aauagccccu      540

gcauauagca uuagauguau aggcguuagc aaucgagauu uuguagaagg uaugucagga      600

gguacuuggg uagauguagu auuagaacac gggggaugug ucaccguaau ggcccaagac      660

aaaccaacug uagauauaga guuaguuacu acaacaguaa gcaauauggc agaagucaga      720

aguuauugcu acgaagcaag cauaagcgau auggcuagug auagccgaug uccuacccaa      780

ggagaggcgu auuuagauaa acaaucugac acgcaauaug uauguaaaag aacucuagua      840

gacagaggau ggggcaacgg augcggauua uuuggaaaag guagcuuagu aacaugugca      900

aaauuugcau guucaaagaa aaugacugga aaaaguauuc aaccagaaaa cuuagaauau      960

cgaauaaugc uaucaguaca uggaagucaa cauucaggaa ugauaguaaa cgacacugga     1020

cacgagacag augaaaaucg agcgaaagua gaaauaacac caaauucacc uagagcugaa     1080

gcaacauuag guggauuugg uagcuuagga uuagauugug aaccaagaac cggucuagau     1140

uucagcgacc uauauuauuu aacaaugaau aauaaacacu ggcuaguaca uaaagaaugg     1200

uuucaugaua uuccucuacc uuggcacgcu ggagccgaca cuggaacacc ucauuggaac     1260

aauaaagaag cguuagucga guucaaagau gcccacgcaa aaagacaaac uguaguagua     1320

uuaggaucac aagaaggagc aguacauaca gcacuagcag gagcacuaga agcagaaaug     1380

gauggagcaa agggaagauu aagcagugga caccuaaaau guagacuaaa aauggacaaa     1440

cuaagauuaa aaggaguuuc uuauagucua ugcacggcag cuuucacauu uaguaaaauu     1500

ccagcugaaa cauuacaugg cacaguaaca gaagaagucc aauaugcagg uacugaugga     1560

ccauguaaag uaccagcaca aauggcagua gauaugcaga cacuaacacc cguagguagg     1620

cuuauaacag cgaauccagu aauaacugaa aguacugaaa auaguaaaau gauguuagaa     1680

uuagaucccc cauuugguga uucauacaua guaauaggag uaggagaaaa gaaaauuaca     1740

caucauuggc accguucagg aucaacaauc ggaaaagccu uugaagcaac uguuagagga     1800

gcaaaacgaa uggcaguuuu aggagauacu gcaugggauu ucggaagugu aggaggggca     1860

uugaauagcu ugggaaaagg uauucaucaa auauucggag cagcauuuaa aagucuauuu     1920

ggugguauga guugguuuag ucaaauacua auuggaacau uacuaaugug gcuagguuua     1980

aauacgaaaa acggaucaau caguuuaaug ugccuagcuc uaggcggcgu ccuaauauuu     2040

uugaguacag caguuuccgc a                                               2061


<210>  2674
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short CDS opt

<400>  2674
acaucuguag gaauaguagg acuacuacua acuacagcca uggcugcaga gguaacaaga       60

agaggaucag cauauuauau guauuuggau aggucagaug ccggugaagc aauaaguuuu      120

ccuacuacau uaggaaugaa caaauguuau auacaaauaa uggauuuagg acauaugugu      180

gaugcaacaa ugaguuauga augcccgaug cuagaugaag gagucgaacc agaugauguc      240

gauuguuggu gcaacacaac aucaacgugg guugucuaug gaacauguca ccacaagaaa      300

ggugaagcua gaagaucaag gagagcaguu acauugccca gucauaguac aagaaaauua      360

caaacaagaa gucaaacaug guuagaaagu agagaauaua cuaaacaucu aauacgugug      420

gaaaauugga uauuuagaaa uccaggauuu gcgcuagcag cagcagcaau agcaugguua      480

cuagguucua guacaaguca aaaaguaaua uaucuaguga ugauauuauu aauugcaccc      540

gcauauagua uaaggugcau aggugucagc aauagagauu uuguggaagg aaugucugga      600

ggaaccuggg uugauguagu uuuagaacau gggggaugcg uuacaguaau ggcacaggau      660

aaacccacag uugauaucga auugguuacg acaacaguua guaacauggc agaaguaaga      720

ucauacuguu augaagcaag uauaucagau auggcaucag auucaagaug uccaacccaa      780

ggagaagcau auuuagacaa acaaagugau acccaauacg uauguaaacg aacucuugua      840

gauagaggau ggggaaacgg auguggauua uuuggaaaag guagucuagu aacuugcgca      900

aaauuugcuu guucaaagaa aaugaccgga aaaucaauac aaccagaaaa cuuagaauau      960

agaauuaugc uaaguguaca uggaucucag cacagcggga ugauaguuaa cgacacaggu     1020

caugaaacag acgaaaauag agcaaaagua gaaauaacac caaacagucc aagagcagaa     1080

gcaacccuug guggauuugg gaguuuaggu cuggauugcg aaccgagaac aggauuagau     1140

uuuagugauu uauauuaucu aacaaugaau aauaaacauu gguuaguaca uaaagaaugg     1200

uuccaugaua uaccacuucc auggcauacg ggagcagaca ccggaacccc acauuggaac     1260

aauaaagagg cauuaguuga auuuaaagac gcacacgcaa aaagacaaac aguaguagua     1320

cugggcuccc aagaaggagc aguacauaca gcacuagcgg gugcacucga agcagaaaug     1380

gauggagcga aaggaagauu aagcaguggc caucuaaaau guagguugaa aauggacaaa     1440

cuaagguuaa aaggaguaag uuacagccuc uguacagcug cuuucaccuu uacaaaaaua     1500

ccggcagaaa cauuacacgg aacaguaaca guagagguuc aauaugcagg aacagaugga     1560

ccauguaagg uuccagcaca aauggcaguc gauaugcaaa cauuaacucc aguuggaaga     1620

uuaauaacag caaauccugu uauaacagaa ggaacagaaa acucaaaaau gauguuagaa     1680

cuagauccac cauuuggcga uaguuauauc guaauaggag uaggggaaaa gaaaauaacu     1740

caucacuggc auagaagcgg aucaaccaua gguaaagcau uugaagcaac gguaaggggu     1800

gcaaaaagaa uggcaguauu gggagacaca gcuugggacu uuggaagugu aggaggugua     1860

uuaaacaguc uaggaaaagg aauacaucaa auauuuggag cagcauuuaa aaguuuauuu     1920

ggaggaauga gcugguuuuc acaaauauua auaggaacau uacuuaugug guugggacua     1980

aauacuaaaa auggaucgau aagccugaug uguuuagcac uagguggagu uuuaaucuuc     2040

cuaaguacag caguaagugc c                                               2061


<210>  2675
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short CDS opt

<400>  2675
acaucaguag gaauaguagg auuacuacua accacagcaa uggcagcaga gguaacaagg       60

agaggaucag cauauuauau guauuuagau cgaucagacg caggagaagc aauaaguuuu      120

ccaaccacgc uaggcaugaa uaaauguuac auucaaauaa uggacuuagg ucauaugugu      180

gaugcaacaa uguccuauga auguccuaug uuagaugaag gaguugaacc ugaugauguu      240

gauuguuggu guaacacaac aucaacuugg guaguauaug guacauguca ccauaaaaaa      300

ggagaagcua gacgaaguag aagagcugua acacuaccaa gccacucgac uagaaaacua      360

caaacaagau cacaaacaug guuagaaagu agagaauaca caaaacaucu uauacgagua      420

gagaauugga uauuucgcaa uccaggauuu gcacuagcag cagcagcaau agcgugguua      480

uuagguucau cuacaagcca aaaaguaaua uauuuaguaa ugauauuauu aauagcacca      540

gcguauucaa uaagauguau aggagucagu aauagggauu ucguagaagg aaugucuggc      600

ggaacauggg uagauguagu acuagaacau ggaggaugcg uaacaguaau ggcucaagau      660

aaaccaacug uugauauaga auuaguaaca acaacaguaa gcaauauggc agaaguaaga      720

ucauacuguu augaggcaag uauaagugau auggcaucag auagcagaug ucccacacaa      780

ggcgaagcau aucucgacaa acaaagcgac acgcaauaug uauguaaacg aacucuagua      840

gauagagguu gggguaacgg cuguggacua uuuggaaaag gaaguuuagu aaccugcgcu      900

aaauucgcau guucaaaaaa aaugacggga aaaucaauac aaccagaaaa cuuagaauau      960

agaauaaugu uaagcguaca uggcagucaa cacucaggaa ugauaguaaa ugauacaggu     1020

caugagacug acgaaaaccg agccaaagua gaaauaacuc caaauucacc aagagcugaa     1080

gcaacacuug gagguuuugg caguuuaggu uuagacugug aaccaagaac aggacuagac     1140

uuuuccgauc uauauuaccu aacaaugaac aauaaacauu gguuaguaca uaaagaaugg     1200

uuucaugaca uaccauuacc uuggcaugca ggagcagaua ccggaacacc ucacuggaau     1260

aauaaagaag cacuaguuga auucaaagau gcacaugcua aaagacaaac ugucguugua     1320

cuagguaguc aagaaggagc aguucauacc gcacuagcgg gagcacuuga agcagaaaug     1380

gacggggcaa aaggaagauu aagcucagga cauuuaaaau gcagacuaaa aauggauaaa     1440

cuaagacuaa aaggagucuc auauaguuua uguacagcag cguuuacauu uacaaagauu     1500

ccagcagaaa cauuacaugg aacaguaacu guagaaguac aguaugcagg aacugacgga     1560

ccauguaaag uuccagcaca aauggcuguu gauaugcaaa cuuuaacucc aguaggaaga     1620

uugauuacag caaaucccgu gauaacagaa uccacagaaa auucuaaaau gauguuagaa     1680

cucgaucccc cguuuggaga uagcuauaua guaauaggag uaggagaaaa aaaaauaaca     1740

caccauuggc acaggucagg uuccacaaua gguaaagcuu uugaagcuac aguaagagga     1800

gcuaaaagaa uggcuguacu aggagauaca gcaugggauu uuggaagcgu aggcggagca     1860

cuuaauucau uaggaaaagg uauccaccaa auauucggag ccgcuuucaa aucuuuauuu     1920

ggaggaauga guugguuuag ccaaauauua auaggcacuc uacuaaugug guuaggacua     1980

aacacuaaaa auggaucuau aucauuaaug uguuuagcau uaggcggagu ucuaauuuuu     2040

cucucaacag caguaucugc u                                               2061


<210>  2676
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short CDS opt

<400>  2676
acaucaguag gaauaguugg auuauuauua acaacagcaa uggcaguaga aguaacuaga       60

agaggaaaug cauauuacau guacuuagau cgaucagaug ccggugaagc gauuagcuuu      120

ccaacaacca ugggaaugaa caaaugcuac auacaaauaa uggaccuagg ccauaugugc      180

gacgcuacaa ugucauacga auguccuaug uuagaugagg gaguagaacc ugaugauguu      240

gacuguuggu guaauacaac aaguacuugg guaguauaug gaacaugcca ucauaagaaa      300

ggagaagcaa ggagaucaag acgagcagua acacuuccaa gccauucaac uagaaaacuu      360

caaacaagau cucaaacgug gcuagaauca agagaauaua caaaacacuu aauaagagua      420

gaaaauugga uuuuccgaaa uccaggcuuu gcauuagcag cagcugcaau agcuugguug      480

cuaggaaguu caacaucaca aaaaguaauu uaccucguaa ugauauuauu aauagcaccc      540

gcauacucaa uucgaugcau aggaguaagu aacagagacu uugucgaagg uaugucagga      600

gggacuuggg uagauguagu acuagaacau ggagguugcg uaacaguaau ggcucaagau      660

aaaccaacag uagacauaga auuagucacu acaacgguau caaacauggc cgaaguacga      720

ucauauuguu augaagcauc aauaucagac auggccagug acuccagaug uccaacacaa      780

ggugaagccu aucuagauaa acaaagugau acgcaauaug uauguaaacg aacacuugua      840

gacagagguu ggggaaacgg cugcgguuua uuugguaaag gaaguuuagu aacaugugcc      900

aaauuugcau guuccaagaa aaugacaggu aaaagcauac aacccgaaaa uuuggaauau      960

cgaauuaugu uaucaguaca cggaucacaa cacucaggua ugauagucaa ugauacuggu     1020

cacgaaacug augaaaauag agcaaaaguc gaaauuacac ccaauucgcc uagagcugaa     1080

gcaacguuag gaggauuugg cucauuagga uuagauugcg aaccaagaac aggucucgau     1140

uucucagacu uauauuacuu aaccaugaau aauaaacauu ggcucguuca caaagaaugg     1200

uuucaugaua uaccucuacc cuggcaugca ggagcagaua ccgguacacc acauuggaau     1260

aacaaagaag cgcuaguaga auuuaaagac gcacaugcaa aacgacaaac uguaguaguu     1320

uuaggaucac aagaaggagc aguacauacu gcauuagcug gagcauuaga agcagaaaug     1380

gauggagcaa aaggaagguu aaguaguggu cauuuaaaau guagauuaaa aauggauaag     1440

uuaagacuaa aaggagugag uuauucacua ugcacagccg ccuuuacauu uacuaaaauu     1500

ccagcagaaa cauugcaugg aacagucaca guggaaguac aauacgccgg aacagacgga     1560

ccaugcaaag ucccagcaca aauggcagua gauaugcaaa cauuaacccc aguaggaaga     1620

cuaauaacag caaauccugu aauaacugaa ucaacggaaa auucaaaaau gauguuggaa     1680

uuagauccac cauuuggcga uucauauaua guuauaggcg ucggagaaaa gaaaauaacc     1740

caucauuggc acagaucagg uucaacaaua ggaaaagcau uugaggccac gguaagaggc     1800

gcaaaaagaa uggcaguauu aggagacaca gcaugggauu ucggaagcgu uggaggagca     1860

cucaauagcu uagguaaagg aauccaucaa auuuuuggag cagcguucaa aucauuauuu     1920

gguggaaugu caugguuuuc acaaauauua auaggaacuu uauuaguaug guuaggacua     1980

aauacuaaaa acggaucuau aagccuaaug uguuuagcac ucggaggagu auuaauuuuc     2040

cuaaguacag caguuagugc u                                               2061


<210>  2677
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short CDS opt

<400>  2677
acaucaguag gaauaguugg ucuauuauug accacagcaa uggcugccga agugacaaga       60

agagguucag cauauuauau guaucuugau cggucugaug caggagaagc gauaaguuuu      120

ccaacaacuc uaggaaugaa caaauguuau auacaaauaa uggaccuagg gcauaugugu      180

gacgcaacaa ugaguuauga auguccaaug cuagaugaag gaguagaacc agacgauguu      240

gacuguuggu guaauaccac caguacaugg guaguauaug gcacauguca ucauaaaaaa      300

ggagaagcgc gacguaguag acgcgccgua acacucccau cacauucaac aagaaaacuu      360

caaacaagaa gccaaaccug guuagaaagu agagaauaca caaaacaccu uauaagagua      420

gaaaacugga uauuuagaaa uccaggcuuc gcauuagcag cugcagcaau cgcaugguua      480

cucggaucau caacauccca aaaaguaaua uaucuaguga ugauacuauu aaucgcacca      540

gcauacucaa uaagauguau aggaguaagu aacagagauu uuguugaagg uaugaguggg      600

gggacauggg uagauguagu acuagaacau ggcggaugcg uaacagucau ggcucaagau      660

aaacccacag uagauauaga acucguaaca acaacaguau caaacauggc agaagucaga      720

ucauauuguu augaggcaag uauaagugac auggcaucag acaguaggug uccaacccaa      780

ggagaagcuu aucuagacaa acaaagugau acccaauaug uauguaaaag aacuuuagua      840

gacagaggau ggggaaaugg augcggauua uuuggaaaag guucguuagu aacgugcgca      900

aaauucgcau guagcaaaaa aaugacuggu aaaucaauac aaccagaaaa ucuagaauac      960

agaauaaugc ucucugugca cgguagucaa cauucuggaa ugauaguaaa ugauacagga     1020

caugaaacgg augagaacag agcaaaagua gaaauaacac caaacagucc aagagcagaa     1080

gcaacacuag gggguuucgg uucuuuagga uuagauugug aaccaagaac aggacuagau     1140

uuuagcgauc uauauuauuu aacaaugaac aacaaacauu gguuaguaca uaaagaaugg     1200

uuucaugaua uaccacuccc auggcaugca ggcgccgaua caggcacacc acauuggaau     1260

aacaaagaag cacuagugga auuuaaagau gcacacgcca aaagacaaac uguaguagua     1320

cuaggaucac aagaaggagc uguacauaca gcauuagcag gugcacuaga ggcagaaaug     1380

gauggagcaa aaggaagauu aucuagcggc caucuaaaau gcaggcuaaa aauggauaaa     1440

uuaagacuaa aaggaguguc auacucucuc uguacugcag cauucacuuu uacaaaaaua     1500

cccgcagaaa cguuacacgg aacuguaaca gucgaaguuc aauaugcagg aacagaugga     1560

ccauguaaag uaccagcaca aauggcagua gauaugcaaa cuuuaacucc aguuggccga     1620

cuaauuacag caaauccagu uauuacagag ucaacagaaa auagcaaaau gaugcuagaa     1680

uuagacccac cauucggaga uucauauaua gucauagggg ucggagaaaa aaaaauuacg     1740

caucacuggc acagaagcgg aucaacaaua ggaaaagcuu ucgaagcgac aguaagagga     1800

gcaaaaagaa uggcaguuuu aggggauaca gcaugggacu uuggauccgu cgguggagca     1860

cuaaauaguc uaggaaaagg aauacaucaa auauucggag cggccuuuaa aucgcuauuu     1920

ggaggcaugu caugguuuag ucaaauucuu auagguacuu uacuaaugug guuaggguua     1980

aauacgaaaa acgggucaau aaguuuagua uguuuagcuc uaggaggagu gcucauauuc     2040

cuaucaacag cgguaagugc a                                               2061


<210>  2678
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short CDS opt

<400>  2678
acaaguguag gaauuguagg auuauuacug acaacugcua uggcagcaga gguuacaaga       60

agaggaagcg cauauuauau guaucuagau agaucagaug cuggugaagc aauuucuuuu      120

ccaacaacau uaggaaugaa uaaauguuac auacaaauua uggauuuagg acauaugugu      180

gacgcuacca ugaguuauga auguccaaug uuagaugaag gaguugaacc agaugacgua      240

gauuguuggu guaauacaac aucaacaugg guaguauaug ggacaugcca ucauaagaaa      300

ggagaagcaa gacgaaguag acgagcugua acauugccau cacauucaac aagaaaauug      360

caaacuagau cacaaacuug gcuagaaagu agagaauaua caaaacauuu aauccgagua      420

gagaauugga uauuccgaaa uccuggauuu gcauuagcag cagcagcaau agcguggcuu      480

cucgguaguu caacaucaca aaaaguuaua uacuugguca ugauauuauu aauagcuccg      540

gcauauagca ucagauguau cggaguaaau aaucgagauu ucguagaagg aaugucaggu      600

ggaacguggg uagauguagu auuagaacac gguggaugug uaacuguaau ggcacaagau      660

aaaccaacug uugauauaga acucguaaca acgacaguaa guaauauggc ggaaguacga      720

aguuauuguu augaagcauc cauaucugau auggcaucag acagcagaug uccaacccaa      780

ggagaagcau accuggauaa acaaucagau acgcaauaug uauguaaaag aacuuuaguu      840

gacagaggau ggggaaaugg augcggcuua uucggcaaag gaaguuuagu aacaugcgca      900

aaauuugcgu guaguaagaa aaugacagga aaaagcauac agccagaaaa ccuagaauau      960

aggauaaugu ugaguguaca uggcagucaa cauaguggaa ugauagucaa ugauacuggc     1020

cacgaaacag augaaaauag ggcaaaagua gaaaucacac caaauagccc ucgagcagaa     1080

gcaacauuag gaggauuugg aucuuuaggu cuagauugug aaccaagaac aggcuuagau     1140

uuuucagacu uauacuaucu uacaaugaau aacaaacauu ggcuaguaca uaaagaaugg     1200

uuucacgaua uaccacuacc uuggcaugca ggagcagaca cuggaacacc acauuggaau     1260

aacaaagaag cacucguaga auuuaaagac gcacaugcaa aaagacagac agugguaguu     1320

uuaggaucac aagagggagc aguacauacc gcauuagcag gagcucucga agcugaaaug     1380

gauggagcaa aaggaagauu aucaagcgga cauuuaaaau gcagacuaaa aauggacaaa     1440

uuaagauuaa aagguguaag uuacagucua uguacugcag cauuuacauu cacuaaaaua     1500

ccagcggaaa ccuuacaugg aacgguaaca guagaagugc aguacgcugg uacggaugga     1560

cccuguaaag ucccugcaca aauggccgua gauaugcaaa cucuaacacc uguaggacga     1620

cuaauuacag cuaauccagu aauaaccgaa agcacagaaa acucuaaaau gauguuagaa     1680

uuagauccuc cuuuuggaga uucauacauu guuauaggag uaggcgaaaa gaaaaucaca     1740

caccauuggc auagauccgg aaguacaaua ggaaaagcau uugaggccac aguaagaggc     1800

gcaaaaagaa uggcaguacu aggagacaca gccugggauu uugguucugu aggaggagca     1860

uuaaacaguu uaggcaaagg aauacaucag auuuucggag cagcauuuaa aucauuauuc     1920

gggggaauga guugguuuuc acaaauacuu auaggaacac uacuaaugug gcuggguuua     1980

aauacuaaaa auggaagcau aucccuaguu ugccuagcac uaggaggagu ucugauauuu     2040

cuaucaacag cuguaagugc a                                               2061


<210>  2679
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short CDS opt

<400>  2679
acgucaguag gaauaguagg guuacuuuua acgacagcaa uggcaguaga aguaacacga       60

agaggauccg cauauuacau guaucuugac cgaucagaug caggagaagc aauaaguuuc      120

ccaacaacac uaggaaugaa uaaaugcuau auacaaauaa uggauuuagg ucacaugugc      180

gaugcaacaa uguccuauga auguccgaug uuagaugaag guguggaacc ugaugaugua      240

gauuguuggu guaauacuac aucuacaugg guugucuacg gcacauguca ucauaagaaa      300

ggagaagcgc gaaggaguag gagagcagua acacuaccgu cacacagcac aagaaaauua      360

caaacuagau cacaaaccug guuggaaucc agagaauaua caaaacauuu aaucagagua      420

gaaaauugga uauuuagaaa uccaggguuu gcuuuagcag cagcagcaau agcaugguua      480

uuaggaaguu caacuagcca aaaaguuauc uaccuaguaa ugauucuauu aauagcacca      540

gcguauucaa uaagauguau agggguaagu aauagagacu ucguagaagg aaugagugga      600

ggaacuuggg uagacguggu auuagaacau ggaggaugcg uaacagugau ggcacaagac      660

aaaccagcag uagacauaga auuaguaaca acaacaguaa gcaauauggc agaaguaaga      720

uccuauuguu augaagcauc aauaucagau auggcaagcg acucgagaug uccgacacaa      780

ggagaggcuu aucuagacaa acaaagcgau acccaauaug uaugcaaaag aacauuagua      840

gauagaggau gggguaaugg uugcggauua uucggaaaag gaucucuagu cacaugugcu      900

aaauucgcau guucaaagaa aaugacaggc aaaaguauac aaccagaaaa uuuagaauac      960

agaauaaugu uaagcguuca uggaagucaa cauaguggaa ugauaguaaa cgauacggga     1020

cacgaaacgg augaaaauag agcgaaagug gaaauaacac cuaauucacc aagagcagaa     1080

gcuacacuag guggauuugg aucucuagga cuggacugug aacccaggac aggucuagau     1140

uuuagcgacc uauauuacuu aacgaugaau aauaaacauu ggcuaguuca uaaagaaugg     1200

uuucaugaca uaccauuacc auggcaugcu ggagccgaca cagguacacc acauuggaau     1260

aauaaagaag cauuaguaga guucaaagau gcucaugcca aaaggcagac aguuguugua     1320

cuagggucac aagaaggagc aguacauaca gcucuagcgg gagcacuaga agcagaaaug     1380

gauggagcaa aagggcgauu aagcucugga cauuuaaaau guagauuaaa aauggauaaa     1440

uuaagauuaa aaggaguaag uuauucauua ugcacagcgg ccuuuacauu cacaaaaaua     1500

ccagcagaaa cacuacaugg gacagugaca guagaaguuc aauaugcagg aacagacgga     1560

ccuugcaaag uuccggcaca aauggcaguu gauaugcaaa cacuaacacc agugggaaga     1620

uuaauaacag caaacccagu uauaacagaa aguacagaaa auagcaaaau gaugcuggaa     1680

uuagauccac cauuugguga caguuacaua guuauaggag ugggagaaaa gaaaauaaca     1740

caucauuggc auagaucugg gagcacaauu ggaaaagcgu ucgaagccac aguaaggggu     1800

gcaaaaagaa uggcaguauu aggagauacu gcaugggauu uuggaucagu agggggugcu     1860

uuaaauucau uaggaaaagg aauacaucaa auuuucggug cagcauuuaa aaguuuauuu     1920

ggaggaaugu caugguucag ucaaauccuc auuggaaccu uauuagucug gcugggauua     1980

aauacuaaaa acgguucuau aaguuuaacc ugucuagcac uaggaggcgu auuaauuuuc     2040

uuaagcacag caguuagcgc a                                               2061


<210>  2680
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short CDS opt

<400>  2680
acgucaguag ggauagucgg auuauuauua acaacugcua uggcaguuga aguuacaaga       60

agaggaagug cauacuauau guaucuugau agaucagaug cgggugaagc aauaucauuu      120

ccuacuacac uuggaaugaa caaauguuau auacaaauaa uggaucuagg acauaugugu      180

gaugcaacaa ugagcuacga auguccgaug uuagacgaag gaguagagcc agacgaugua      240

gauuguuggu guaacacaac aucaacaugg gucguuuaug gaacguguca ccacaaaaaa      300

ggagaagcaa gaagaucgcg aagagcaguu acacuaccaa gccauaguac cagaaaacua      360

caaaccagau cacaaacaug gcuagaauca cgugaauaua caaaacaucu aauacgaguu      420

gaaaauugga uauuuagaaa uccagguuuu gcacuagcag cagcaguaau agcauggcua      480

cuaggaucgu caacaaguca aaaagucaua uacuuaguca ugauccuacu aauagcacca      540

gcuuacucca uaagauguau aggaguaagc aacagggauu uuguugaagg uaugaguggc      600

ggaacauggg uugauguugu acuagaacac ggggguugug uaaccguuau ggcacaagac      660

aaaccaacag uagacauaga acugguaaca acaacaguau caaauauggc agaaguucga      720

aguuauuguu augaagcaag uauuagcgac auggcuagug auucaagaug uccaacacaa      780

ggugaagcau auuuagauaa acaaucugau acacaauacg ucuguaaaag aacacuuguc      840

gacagaggau ggggaaaugg augugguuua uuuggaaaag gaagccucgu aacuugugcu      900

aaauuugcau guaguaaaaa aaugacggga aaaaguauac aaccagaaaa uuuagaauau      960

agaauaaugu uaagugugca cggaucacag cauucaggua ugauuguaaa cgacacagga     1020

caugagacug acgaaaauag agcaaaagua gaaauaacac cgaacagccc uagagcagaa     1080

gcaacacuug gagguuuugg aucauuaggu cuugauugug aaccuagaac agguuuagau     1140

uucagcgacu uauauuaucu aacaaugaac aauaaacauu gguuagugca caaagaaugg     1200

uuucacgaca uaccgcugcc auggcaugca ggagcagaca caggaacacc ucauuggaau     1260

aauaaagaag cgcuaguuga auuuaaagau gcacacgcaa aaagacaaac aguaguugua     1320

cuaggaagcc aagaaggcgc aguucauaca gcguuagcag gagcucuaga ggcagagaug     1380

gauggcgcaa aagggagauu aaguagugga cauuugaaau guagacuaaa aauggauaaa     1440

uuaagauuaa aaggaguauc auauagccua ugcacagcag ccuuuacauu uacaaaaaua     1500

ccagcagaaa cauuacaugg aacuguaacu guggaaguac aauaugcagg aacagauggc     1560

ccauguaaag uaccagcaca aauggcuguc gacaugcaaa cacuaacacc aguuggaagg     1620

uugauaacug caaauccagu gauuacagaa ucaacagaaa auucaaaaau gauguuagag     1680

uuagacccac cauuuggaga uagcuacaua guaauuggcg uaggagaaaa aaaaauuacg     1740

caucauuggc acagaagcgg auccacaaua ggaaaagcau uugaagcaac aguaagagga     1800

gcaaaaagga uggcaguguu aggagauaca gcaugggauu uuggaagugu cggaggugcu     1860

cuaaacucau uagguaaagg uauacaucaa aucuuugggg cagcauuuaa gucgcuauuu     1920

ggaggaauga gcugguuuag ccaaaucuua auugguacac uacuagucug gcuaggauua     1980

aacacaaaaa augguucaau aagcuuaacc ugucuagcau uagggggcgu acuaaucuuc     2040

uuaucaacag caguaagugc a                                               2061


<210>  2681
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short CDS opt

<400>  2681
acaucagugg guaucguagg auuauuacuu acaacagcaa uggcaguuga aguaacaagg       60

aggggauccg cauauuauau guacuuagac agaagugacg ccggugaagc aauaucauuu      120

ccaacaacac uaggaguuaa uaaauguuau auacaaauua uggaccuagg acacaugugu      180

gaugcaacaa uguccuacga auguccaaug cuagacgaag gaguggaacc agaugaugua      240

gauuguuggu guaauacaac aaguacaugg guaguuuaug ggacauguca ucauaagaaa      300

ggagaagcac gaagaucaag gagagcuguc acauuaccau cacacaguac gagaaaacua      360

caaacaagaa gucaaacaug gcuagaauca agggaauaua cuaaacaucu uauaaggguu      420

gaaaauugga uauucagaaa uccaggauuc gcacuagcag cugcagcuau agcguggcuu      480

uuggguagua guacuagcca aaaagucaua uauuugguua ugauucuacu aauugcaccg      540

gcauauucca uacguuguau aggaguauca aauagagacu uuguugaagg aaugucagga      600

ggaaccuggg uagauguagu acuagaacac ggaggcugug uuacggucau ggcacaagau      660

aaaccaacag uagauauaga acugguuaca acaacaguaa gcaauauggc ugaaguacga      720

agcuacugcu acgaagcaag uauaucagau auggcaucag acucacgaug uccaacacaa      780

ggagaagcau auuuagauaa acaaagugau acucaauaug uauguaaaag aacucuagua      840

gauagaggau gggggaaugg gugcgguuua uucggaaaag guagucuagu uaccugugcc      900

aaauuugcau gcucaaagaa aaugacugga aaaaguauac agccugaaaa uuuagaauau      960

agaauaaugc uaucaguaca uggaagucaa cauuccggaa ugauagucaa ugauacagga     1020

caugaaacag augaaaauag agcaaaagua gaaauuacac caaacagucc aagagcagaa     1080

gcaacauuag gagguuuugg uucauuagga uuagauugcg aacccagaac aggacuagac     1140

uuuagugacu uauauuaccu aacaaugaau aauaaacauu gguuagugca uaaagaaugg     1200

uuucaugaua uaccauuacc auggcacgca ggagcugaua cgggaacacc acacuggaau     1260

aauaaagagg cucugguaga auuuaaagau gcacacgcaa aaagacaaac aguaguuguu     1320

uuggguagcc aagaaggugc aguucacaca gcguuggccg gagcauuaga ggccgaaaug     1380

gauggugcaa aaggaagacu aucaagugga caucuaaaau guagacuaaa aauggacaaa     1440

uuaagauuaa aaggaguaag uuauagccua ugcacagcug cauuuacauu cacaaaaaua     1500

ccagccgaaa cauuacaugg aacaguaaca guagaaguuc aauaugcagg aacagacgga     1560

ccaugcaaag uaccugcaca aauggcuguc gauaugcaaa cauuaacucc aguuggaaga     1620

uugauaacag caaauccagu aauaacagaa aguaccgaaa acagcaaaau gauguuagaa     1680

cuugauccac cuuuugguga uaguuauaua gugauuggcg uaggagauaa gaaaauuaca     1740

caccauuggu auagaucagg aucaacaaua ggaaaagcau ucgaagcaac uguaagagga     1800

gcaaaacgaa uggcaguauu aggagacacu gcaugggauu uuggaagcgu aggaggagcg     1860

uuaaauucac uaggcaaagg aauacaucaa auauuuggag cagcauuuaa aaguuuguuu     1920

ggcggaauga gcugguuuag ucaaauauua auaggaacau uauuaguaug guuaggacua     1980

aacacgaaaa auggaucaau aagucuaaca uguuuagcuc uagguggagu auuaauauuu     2040

uuaaguacag caguaucagc a                                               2061


<210>  2682
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short CDS opt

<400>  2682
acaucaguug gaauaguugg acuauuacuc acuacagcaa uggcaguaga gguaacacga       60

agaggcucag cauauuauau guaccuugau aggucagaug ccggaaaagc aauuucauuu      120

ccaacaacau uagguguaaa caaaugcuau auacaaauaa uggaucuagg ccauaugugu      180

gaugcaacua ugucauauga augcccaaug uuagaugaag gaguagaacc agaugaugua      240

gacuguuggu guaauacuac aaguacuugg guaguauaug gaacauguca ccauaaaaaa      300

ggagaagcaa gaagaagcag gagagcaguc acauugcccu cacauucuac aagaaaauug      360

caaacgagaa gccaaacuug guuggaauca agagaauaca caaaacacuu aauaagagua      420

gaaaauugga uauuuagaaa cccaggauuc gcauuggccg cagcagcuau agcaugguua      480

uuaggaucuu cuacgucgca aaagguaaua uaucuaguaa ugauauuauu aauagccccc      540

gcguauucaa uuagauguau uggaguauca aauagagacu ucguagaagg gaugucagga      600

ggaacauggg ucgauguugu uuuagaacac gguggaugcg ucaccguuau ggcacaagau      660

aaaccuacag ucgauauuga acuaguaacu acuacugucu caaacauggc agaaguucga      720

aguuauuguu augaagcauc aauuucagau auggcaucag auaguagaug uccaacccaa      780

ggagaagcuu auuuagacaa acaaagcgac acacaauaug uuuguaaaag aacauuaguu      840

gauaggggcu ggggaaaugg uuguggauua uucggaaaag gaaguuuagu uacaugugca      900

aaauucgcuu guucuaaaaa aaugacggga aaaaguaucc aaccugagaa uuuagaauau      960

agaauaaugc uaucaguaca cggaagucaa cacagcggga ugauaguaaa ugacauagga     1020

caugaaacgg augaaaauag ggcuaaagua gaaauaacac cgaacucacc aagagccgaa     1080

gcaacacuug gugguuucgg aucauuagga cuggauugcg aaccaagaac uggauuagac     1140

uucagcgauc uauauuacuu aacaaugaau aauaaacacu gguuaguaca uaaagaaugg     1200

uuccaugaua uaccauuacc cuggcaugca ggagcagaua cgggaacacc acauuggaau     1260

aacaaagaag cccuaguaga auucaaagau gcacaugcca aaagacaaac aguaguaguu     1320

cuagggaguc aagaaggagc aguacauacu gcacuagcag gagcauugga agcagagaug     1380

gauggagcaa aaggaagauu aucaaguggu cauuuaaaau gcagauugaa aauggauaaa     1440

uuacgauuaa aaggaguauc auacucucuu ugcacagcag cauuuacuuu cacuaaaauu     1500

ccagcugaaa cucuucaugg aacaguaaca guagaagucc aguacgcggg aacagaugga     1560

ccaugcaaag uaccugcaca aauggcagua gacaugcaaa cauuaacccc aguaggaaga     1620

uuaauaacag caaacccugu aauaacagaa aguacugaaa acucuaaaau gauguuagaa     1680

uuagauccac cuuuuggaga uucauauaua gucauaggag uuggagauaa aaaaauaaca     1740

caccacuggc auaggucagg aagcacaauu ggaaaagcau uugaagcuac cguaagaggc     1800

gcaaaaagaa uggcaguauu aggagacaca gcaugggauu ucgguucugu aggaggugcg     1860

uuaaacucau uggguaaagg gauacaccaa aucuuuggag ccgccuuuaa gagccuauuu     1920

ggaggaauga guugguuuag ucaaauacua auaggaacac uauuaguaug guugggacua     1980

aauacaaaaa auggaagcau aucacuuaca uguuuagcau uaggaggugu auuaauauuu     2040

cugucaacug caguuagcgc a                                               2061


<210>  2683
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short CDS opt

<400>  2683
acaaguauag gaauuguagg ucuacuucuc acuacagcaa uggcagcuga gauaacaaga       60

cgggguagug cauacuauau guaccuagac agaagugacg cagguaaagc aauuucauuu      120

gcaacuacau uaggcguaaa caaaugucau guacaaauaa uggauuuagg acauaugugc      180

gacgcaacaa ugucauauga augcccuaug cuggaugagg gaguugaacc ugacgaugua      240

gauuguuggu guaauacaac aucaacaugg guagucuacg gaacaugcca ucauaagaaa      300

ggagaagcaa gaagaaguag gagagcagug acacuaccuu cucauaguac aagaaaauua      360

caaacuagaa gucaaacaug gcuagaauca agagaauaua caaaacauuu aauaaaaguu      420

gaaaauugga uauuuagaaa uccaggauuu acauuaguag cuguugcaau agcauggcua      480

uugggaucaa guaccaguca aaaaguuauu uaucuaguaa ugaucuuauu aauagccccu      540

gcauauagca uacgauguau aggaguuuca aacagagauu uuguagaagg aaugagugga      600

gguaccuggg uagauguagu auuagaacau ggaggcugcg uaacggugau ggcucaagau      660

aagccaacag uugauauaga auuaguaaca acaacaguaa gcaacauggc agaaguuaga      720

agcuacuguu acgaagcaag uauaagugau auggcaucag auaguagaug cccaacgcaa      780

ggagaagccu acuuagauaa acaaagugau acacaauaug uaugcaaaag aacauuaguu      840

gaucgaggau ggggaaacgg uugcgguuua uuuggaaaag gaucuuuagu aacuugugca      900

aaguuuacau guaguaagaa aaugacuggu aaaagcauac aaccagaaaa ucuagaauau      960

agaauaaugu uaucaguaca uggaagccaa cacagcggaa ugauaguuaa ugacaccgga     1020

cacgaaacag acgaaaauag agccaaagua gaaguuacac caaacagucc ucgagcggaa     1080

gcaacacuag gcggauucgg aaguuuagga uuagacugug aaccuagaac aggccuagau     1140

uuuagugacu uauacuacuu aacaaugaau aacaaacauu gguuaguaca caaagaaugg     1200

uuccaugaca ucccacuacc auggcacgcu ggugcagaua cuggaacacc acauuggaau     1260

aauaaagaag cauuaguaga auuuaaagau gcacaugcua aaagacaaac aguuguuguu     1320

uuaggaucuc aagaaggagc aguacauaca gcacuagcug gcgcucugga ggcugaaaug     1380

gauggagcga aaggaagauu auucucagga cacuuaaaau guagauuaaa aauggauaaa     1440

uugagacuaa agggaguaag uuauagucua uguacagcag cauuuacguu uacaaaagug     1500

ccugcagaaa cacuacaugg aacugucacg guagaaguuc aauaugcagg aacugaugga     1560

ccguguaaag uaccagcaca aauggcagua gauaugcaaa cauuaacccc uguaggaaga     1620

cugauaacag caaacccggu aauaacagaa aguacagaaa acaguaaaau gauguuagaa     1680

uuggacccgc cauucggaga uagcuauaua guaauuggcg uaggagauaa gaaaauaacu     1740

caccauuggc acagaagugg aucaacaaua ggaaaagcau ucgaagcaac aguaagagga     1800

gcaaaacgaa uggcaguauu gggagauaca gcaugggauu uuggaucagu aggaggcgua     1860

uuuaacucgc uaggaaaagg gauacaucaa aucuuuggag cagccuucaa aaguuuauuu     1920

ggaggaauga gcugguuuag ucaaauacuu auaggaacac uauuaguaug guuaggauua     1980

aacacuaaaa acggaaguau cucuuuaaca uguuuagcau uaggaggagu gaugauauuc     2040

uuaucgacag cugugagcgc g                                               2061


<210>  2684
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short CDS opt

<400>  2684
acaaguauag gaauaauagg ucucuuguua acgacagcua uggcagcaga aauaacaaga       60

cgagggagug cauauuauau guaccuagac cguucugacg ccggaaaagc uauaucauuu      120

gccacaaacc ucggaguaaa caagugucau guacaaauaa uggaucucgg ucauaugugu      180

gacgcgacua ugaguuauga auguccaaug cuagaugaag gaguagaacc agacgacguu      240

gauugcuggu guaauacaac aagcacuugg guaguauaug gaacauguca ucacaagaaa      300

ggggaggcca gaagaucaag acgagcagua acauuaccau cccacagcac aagaaaauua      360

caaacaagaa gucaaacaug guuagaaagu agagaauaua cuaaacauuu aauaaaaguu      420

gaaaauugga uuuucagaaa uccaggauuc gcauuagcag caguagcaau agccuggcua      480

uuaggaucau caacuucaca aaaaguaauu uaucugguca ugauauuacu aauagcacca      540

gcauauagca uaagaugcau aggaguaagu aacagagauu uuguagaagg aaugagugga      600

ggaacauggg uugaugucgu auuagaacau ggagguugug uaacuguuau ggcgcaagau      660

aaaccaacag uagacaucga auuaguuaca acaacaguaa gcaauauggc ugaaguaagg      720

aguuauuguu augaagcauc cauuucggau auggccucag auaguagaug cccuacacaa      780

ggagaagcau auuuagauaa acaaucagac acacaauaug ucugcaaacg aacucuaguc      840

gauagaggau gggguaaugg cuguggccua uucgguaaag gaucccuagu aacaugcgca      900

aaauucacau gcucuaagaa aaugacaggg aaaucaaucc aaccugaaaa uuuggaauau      960

cgaauaaugu uaaguguaca cgggagucaa cacucaggaa ugauaguaaa ugauauaggg     1020

caugaaacag augaaaacag agcaaaagua gaaguaacgc cuaauucacc aagagcugaa     1080

gcaacacuag guggauuugg gaguuuagga uuagacugug aaccaagaac aggauuagau     1140

uucucagacc ucuacuacuu aacaaugaau aauaaacacu ggcuaguaca uaaagaaugg     1200

uuccaugaua uuccuuuacc auggcacgca ggagcagaua caggcacacc ucauuggaac     1260

aauaaagaag cucuaguaga auuuaaagau gcacaugcaa aaagacaaac ugugguugua     1320

uuagguucac aagaaggagc aguacauaca gcacuagcug gcgcauugga agcagaaaug     1380

gacggagcaa aagguaggcu cuuuagugga caucuaaaau guagguuaaa aauggacaaa     1440

cuuagguuaa aaggaguauc auacucacua ugcacugcug cuuucacauu cacaaaagua     1500

cccgcagaaa cauuacacgg aacagugaca guugaaguac aauaugcugg aacugauggu     1560

cccuguaagg uaccagcaca aauggccgua gauaugcaaa cauuaacucc aguagguaga     1620

cuaauaaccg caaauccagu aauuacagaa uccacagaaa auaguaagau gaugcuagag     1680

cuagauccac cauuuggaga uaguuauaua gucauaggag uaggugacaa gaaaaucaca     1740

caucacuggc auagaagugg cagcacaauu ggaaaagcau uugaagcaac aguaagagga     1800

gcaaaaagaa uggcaguauu aggugauaca gcaugggauu uuggaagugu agguggaguu     1860

uuuaauucac uaggaaaagg aguacaucaa auauuuggag ccgcuuucaa aucacuauuu     1920

ggaggaauga guugguuuuc ucaaauauua auaggaacau uauuaguaug guuaggucua     1980

aacacuaaaa auggaucaau cucauuaaca uguuuagcac ucggaggagu uaugauuuuc     2040

cuaagcacag caguaagcgc a                                               2061


<210>  2685
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short CDS opt

<400>  2685
acuucaauag guauaauagg ucuauuacua acaacagcaa uggcugcaga aauaacacga       60

agaggaucag cuuacuacau guaucuagac agaagugaug cuggaaaagc aauuagcuuc      120

gcaacaacac ucggaguuaa uaaaugccac guucaaauaa uggacuuagg acauaugugu      180

gaugcaacua ugaguuacga auguccaaug cuugacgaag guguugaacc agaugaugua      240

gacuguuggu guaauacgac uagcacgugg guaguauacg gaacauguca ucauaaaaaa      300

ggcgaagcua gaagaagucg acgagcaguu acacuaccga gucacaguac aagaaaacua      360

caaacacgau cacaaacaug guuagaaagu agagaauaua caaaacaccu aauaaagguu      420

gagaauugga ucuucagaaa uccgggguuc gcucuuguag caguagccau agcaugguua      480

uuaggaucua gcacaucaca aaaaguaauc uaucuaguua ugauacuuuu gauagcacca      540

gcuuauagua uaagauguau uggaguauca aauagagacu ucguagaagg aaugucugga      600

ggaaccuggg uagauguagu auuagaacac ggcggaugcg uuacaguaau ggcccaagau      660

aaaccaacag uagacauaga auuaguaacc acgacaguaa guaauauggc ggaagugaga      720

aguuauugcu augaagcaag uauaucagau auggcaucag auucacgaug cccaacacaa      780

ggagaagccu auuuagauaa acaaagcgau acacaauacg uuugcaaaag aacucuagua      840

gacagaggau ggggaaaugg auguggauua uucggaaaag gaucuuuagu aaccugugca      900

aaauuuacau gcaguaaaaa aaugacaggu aaaaguauac aaccagaaaa uuuagaauau      960

agaaucaugc uuucaguaca cggaucacaa cauagcggua ugauaguaaa cgacauagga     1020

caugaaaccg augaaaauag ggcaaaagua gagguaacac caaacucacc aagagcagaa     1080

gcuacuuuag gaggauuugg aucauuaggg uuggauugug aaccacgaac cggacuagau     1140

uuuagugauc uauacuaucu aacaaugaau aacaaacauu gguugguaca uaaagaaugg     1200

uuucacgaca uuccucuacc auggcaugcu ggugcagaua cuggaacacc acauuggaau     1260

aauaaagaag cacuaguaga guuuaaagau gcccaugcca aacgacaaac aguaguagua     1320

cuaggaaguc aagaaggagc aguacauaca gcauuagcug gagcauuaga agcggaaaug     1380

gauggagcaa aaggaagacu auucaguggu caccuaaaau guagauuaaa aauggauaaa     1440

uuaagauuaa aaggaguguc auauucccua ugcacagcgg cauucacauu uacgaaaguu     1500

ccagcagaaa cacuccaugg aacagugaca guagagguac aauacgcugg aacugauggu     1560

ccaugcaaag uaccggcaca aauggcgguu gauaugcaga cauuaacacc aguagggagg     1620

uuaauaacag caaauccagu aauuacagaa agcacagaaa acucuaaaau gaugcuagag     1680

uuagauccac ccuuuggaga caguuacauc guaauaggag uaggagauaa aaaaauaacg     1740

caucauuggc auagaucagg uucuacuauu ggcaaagcuu uugaggcaac aguaagagga     1800

gcaaaaagga uggcagucuu aggugauacg gcaugggauu uuggcucugu aggaggaguu     1860

uuuaacaguu uagguaaagg aauucaccaa auauuuggag cggcauuuaa aagcuuauuu     1920

ggaggaauga guugguuuuc acaaauacuu auagguacgu uauuagucug gcuuggauua     1980

aacacaaaaa auggaaguau aucacuaacu ugcuuagcau uaggaggcgu aaugauauuu     2040

uuaaguacug caguaagugc u                                               2061


<210>  2686
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short CDS opt

<400>  2686
acaagcauug gaauuauagg acuauuauua acaaccgcaa uggccgcaga aauaacgaga       60

cguggaagcg cauacuauau guauuuagac cgaucugaug caggaaaagc uauuucuuuu      120

gcgacaaauu uaggaguuaa uaaaugccau guucaaauaa uggaucuagg acauaugugu      180

gaugcaacaa ugucauauga auguccaaug uuagaugaag gaguagaacc agacgacgua      240

gauuguuggu gcaacacaac aucaacaugg guaguauacg gaacguguca ccauaagaaa      300

ggugaagcaa gaagaucuag gagagcagua acauuaccau cgcauagcac aagaaaacua      360

caaacaagaa gucaaaccug gcuagaauca agagaauaca caaaacaccu cauaaaagug      420

gaaaauugga uauucagaaa cccaggauuc gcuuuagcag caguagcaau agcauggcuu      480

uuaggaagca guacauccca aaaaguaaua uauuuaguua ugauauuauu aauagcucca      540

gcauacucaa ucaggugcau aggaguaagu aauagggauu ucguugaagg aaugucagga      600

ggaacauggg uagacguagu auuagagcac ggagguugcg uaacgguaau ggcacaagac      660

aaaccaacag uggauauaga auuaguaaca acaacuguua guaauauggc ggaaguacga      720

ucauauugcu augaggccuc uauaucagac auggcaucag acagcagaug cccaacccaa      780

ggagaggcuu acuuagauaa acaaagugac acacaauacg uauguaaaag aacacucguu      840

gaucgaggcu ggggaaaugg augcggauua uuuggaaaag gaucuuuagu uacaugcgca      900

aaauuuacau guaguaagaa aaugacaggg aaaagcauac aaccagaaaa uuuagaauau      960

agaauaaugc uaucuguaca uggaucacaa cauucaggga ugauaguaaa cgaugaaaac     1020

cgagcaaaag ucgaaguaac uccaaauuca ccucgagcgg aagcaacguu aggugguuuc     1080

gguaguuuag guuuagauug cgaaccuaga acaggacugg auuucagcga uuuauauuac     1140

cuaacuauga acaauaaaca uugguuagug cauaaagaau gguuucacga uauaccacuc     1200

ccauggcacg caggagcuga uacaggaaca ccacauugga auaauaaaga agcucuagua     1260

gaauuuaaag augcacacgc aaaaagacaa acaguagugg uacuaggaag ucaagaagga     1320

gccguacaua cagccuuagc aggagcauua gaagcugaaa uggauggugc aaaaggaagg     1380

uuauuuucag gacauuuaaa auguagacuc aaaauggaua aauuaagauu aaaaggugua     1440

aguuauucac uaugcacagc agcuuuuaca uuuacaaaag uaccagcaga aacacuacac     1500

gggacaguga caguagaagu acaguacgca ggaacagaug ggccauguaa aguaccagca     1560

caaauggcug uagacaugca gacauugaca ccgguuggaa gauuaauaac agcaaaucca     1620

guaauaacug aaaguacuga aaauucaaaa augaugcuag aacuagaccc accauuuggc     1680

gacuccuaua uaguuauagg agugggugau aagaaaauca cacaucauug gcauagauca     1740

ggaaguacga uaggaaaagc guuugaagcc acgguuagag gagcuaaaag aauggcagug     1800

cuaggagaua cggcuuggga uuuuggaagu guaggaggcg uguuuaauag ccuagggaaa     1860

ggaauacauc aaauauuugg ggcagcuuuc aaaagucuau uuggagguau gaguugguuu     1920

agucaaauau uaauagguac acuauuagua ugguuaggau ugaacacuaa aaaugggagc     1980

auuaguuuga cuuguuuagc acuagguggu guaaugauau uuuuaucaac agcaguaagu     2040

gca                                                                   2043


<210>  2687
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short CDS opt

<400>  2687
accucaauug guauugucgg acuacuccua acaacagcaa uggcagcaga aaucacucga       60

aggggaucgg cguacuauau guaucuugau aggagcgacg caggaaaagc aaucucguuu      120

gcuacaacau uaggaguaaa caaaugccau guacaaauaa uggauuuggg acauaugugc      180

gaugcaacaa uguccuauga guguccuaug uuggacgaag gaguugaacc cgacgacgua      240

gacuguuggu guaauacgac aagcacaugg guuguauaug guacauguca ucauaaaaaa      300

ggugaagcua gaagaucucg gcgagcagug acauuaccau cacauucaac aaggaaacua      360

caaacuagau cacaaacaug guuagaauca cgagaauaua cgaaacaucu aaucaaagua      420

gaaaacugga uuuuucguaa cccaggauuu gcccuuguag caguagcaau agcauggcuu      480

cuaggaucga guacauccca aaaaguaauu uauuuaguaa ugauacuauu aauagcacca      540

gcauauagca uaagauguau aggaguuagu aacagagacu ucgucgaagg aaugucaggc      600

ggaacauggg uugauguagu ccuagaacac ggcggaugug uaacaguaau ggcucaagau      660

aaaccaacag uagauauaga acucgucacg acaacaguau caaauauggc cgaaguaaga      720

aguuauuguu augaagcgag uauuagugac auggccucag auucaagaug uccaacccag      780

ggcgaagcau aucuugauaa acaaucugac acacaauaug uaugcaaaag aacacuugua      840

gacagaggau ggggaaaugg augcggacua uuuggaaaag gaagccuagu aacaugugca      900

aaauucacuu guucaaaaaa gaugacagga aaaagcauuc aaccugaaaa cuuagaauau      960

cgaauaaugu uaucuguuca uggaucacaa cauucuggaa ugauaguaaa ugacacugga     1020

caugaaacug acgaaaacag agcaaaagua gaagucacac ccaauucacc aagagcagaa     1080

gcuacacuag gaggauuugg uagucuaggu cuagauugug aaccaagaac uggacuagau     1140

uucucagacu uauacuaucu gacaaugaau aacaaacacu gguuaguaca uaaggaaugg     1200

uuucaugaca uaccucuccc uuggcacgca ggagcagaua cagguacccc acauuggaac     1260

aauaaagaag cacuaguaga auuuaaagac gcccaugcaa aaagacaaac aguaguaguc     1320

uuaggaagcc aagaaggagc aguacauaca gcauuagcag gagcucuaga agccgaaaug     1380

gacggagcaa aagguagacu auuuucuggu caucuaaaau gccgacuaaa aauggacaaa     1440

uuaagacuaa aagguguauc auacagcuua ugcacagccg cauuuacauu cacuaaaguc     1500

ccagcagaaa cauuacacgg gacaguaaca guagaaguac aauaugcagg aacagacgga     1560

ccaugcaaag ucccagcaca aauggcagua gacaugcaaa cauuaacucc aguaggaaga     1620

cuaauaacag cuaacccagu uauuacagaa ucgacagaaa auucaaaaau gaugcuagaa     1680

cuugauccac cauuugguga uucauauaua guuauaggag uaggagauaa aaaaauaaca     1740

caucauuggc auagaucagg aucaacaaua ggaaaagcau uugaagcaac aguaaggggc     1800

gccaaaagaa uggcaguauu aggagacaca gcaugggauu uugguucugu uggaggugua     1860

uucaauucau uaggaaaagg aauccaccaa auauuuggag ccgcauuuaa aucucuauuu     1920

ggaggaaugu caugguucuc acaaauauua auaggaacau uguuaguaug gcuaggauua     1980

aacacgaaaa augggucaau uagucuaacc ugucuagcau ugggaggagu aaugauauuu     2040

cuaucaacag caguaucagc a                                               2061


<210>  2688
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short CDS opt

<400>  2688
acaucuaucg guauaguagg auuacuauua acaacggcaa uggccgcaga gauaacucga       60

agaggaucag cguacuauau guacuuagau aggucugacg ccggaaaagc aauaaguuuu      120

gcuacuacau uaggaguaaa uaaaugucac guacaaauaa uggaucuagg acauaugugu      180

gacgcuacua ugucauauga augcccaaug uuagaugaag gcguugaacc ggaugaugua      240

gauuguuggu gcaauacuac aucaacaugg guaguauaug gaacauguca acauaagaaa      300

ggugaagccc gaagaagucg gagagcuguc acauuaccau cacacucaac aagaaaauua      360

caaacaagau cacaaacaug guuagaaucc agagaauaca caaaacaucu aauaaaaguu      420

gagaacugga uuuuuagaaa cccaggcuuu gcuuuaguag caguggcaau agcgugguua      480

uugggaagua guacaaguca aaaagugaua uauuuaguaa ugauucuauu aauagcucca      540

gcauauagua ucagauguau agguguauca aauagagauu uuguagaagg aaugagcgga      600

ggaaccuggg uagaugucgu auuagaacau ggaggaugug uaacuguuau ggcacaagac      660

aaaccuaccg uagauauaga auuaguaaca acuacuguua guaauauggc ugaagucaga      720

agcuauugcu acgaagcaag uauaagugau auggcaagug auucgagaug uccaacucaa      780

ggcgaagcau aucuugauaa acaaagcgau acacaauaug uauguaaaag gacguuggua      840

gauagagguu ggggaaacgg auguggcuua uucggaaaag gaagucuagu uacuugcgcg      900

aaauuuacau guagcaagaa aaugacaggu aaaaguauac aacccgaaaa ucuagaauau      960

agaaucaugu uaucaguaca cggaucacaa cauaguggaa ugauaguaaa ugauauagga     1020

cacgaaaccg augaaaauag agccaaaguu gaaguaacgc caaauucgcc aagagcugaa     1080

gcaacauuag gaggauucgg aucucuugga uuagauugcg agccaagaac aggauuagac     1140

uuuagugauu uauacuaucu aacuaugaau aacaaacauu gguuaguaca uaaagaaugg     1200

uuucaugaua ucccauuacc uuggcaugca ggagcagaua cagggacacc ucauuggaau     1260

aauaaagaag cauuagucga guucaaagac gcucaugcaa aaagacaaac cguaguagua     1320

cuaggaucac aagaaggagc cguacauacg gcgcuugcag gagcauuaga agcagaaaug     1380

gauggagcaa aaggaagauu auuuucugga cauuuaaaau guagauuaaa aauggauaaa     1440

cugagauuaa aaggaguuuc uuauucuuua uguacagcag uuuguacagc agcuaaaguc     1500

ccagcagaaa cacuacaugg aacaguaaca guagaaguac aauaugcagg aaccgauggu     1560

ccauguaaag uaccagcuca aauggcaguc gauaugcaaa cauuaacucc gguaggaaga     1620

cuaauuacag cuaacccagu aauaacagag ucaaccgaaa auagcaaaau gauguuagaa     1680

uuagauccac cauuuggaga uagcuacauu guaauaggag uuggagauaa gaaaauaaca     1740

caucauuggc auagaucggg aagcaccauu gggaaagcau ucgaagcgac agugagggga     1800

gcaaaaagaa uggcaguauu aggagauaca gcgugggauu uuggaucugu aggaggagua     1860

uuuaauaguu ugggaaaagg aauacaucaa auauuuggag cagcauuuaa aucauuauuu     1920

ggaggaauga gcugguuuuc acaaauauua auaggcacac uauuggucug gcucggacug     1980

aauacuaaaa acggaaguau uaguuuaacg ugccuagcau uaggaggcgu aaugauauuc     2040

cuuaguacag caguuucagc a                                               2061


<210>  2689
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short CDS opt

<400>  2689
accucaauag gaauaguagg cuuguuguua acaacagcaa uggcagccga aauuacaaga       60

agagguuccg cauauuauau guaccuagau agaucagaug cagggaaagc aauaaguuuu      120

gcaacaacuu uaggagucaa uaaaugucau guacaaauaa uggauuuggg acauaugugu      180

gaugcaacaa ugagcuacga auguccaaug uuagaugaag gaguugaacc ugacgaugua      240

gauuguuggu guaauacaac aaguacaugg guaguauacg gaaccuguca ucauaaaaaa      300

ggagaagcuc gaagaagcag aagggcugua acacuaccgu cucauagcac uagaaaauua      360

caaacacgau cccaaacaug gcuagaaagu agagaauaca caaaacauuu aauaaaagua      420

gaaaauugga uauuuagaaa ucccggauuc gcuuuaguag ccguagcaau ugcaugguua      480

uuaggaucau cuacaucaca aaaaguaaua uaucuuguaa ugauacuauu aauagcgccg      540

gcauauagua uuagaugcau aggaguaagu aauagagauu uuguagaagg aaugaguggu      600

gguacguggg uagaugucgu ucuagaacac ggaggaugcg uaacaguaau ggcacaagac      660

aaaccgaccg uagacauaga auuaguuaca acuaccguuu caaacauggc cgaagugaga      720

ucuuacuguu augaagcuuc aauaagugau auggcaucag auucaagaug cccuacacaa      780

ggagaagcgu accuugacaa acaaucggau acacaauaug uaugcaaaag aacacuagua      840

gaucgaggau ggggaaacgg uuguggacug uuuggaaaag gaaguuuggu aacuugugca      900

aaauuuacau guucaaaaaa aaugacaggu aagucaauac aaccagaaaa ccuugaguau      960

agaauaaugc uaucaguaca uggaagucag cacucaggaa ugauaguaaa ugacgaaaac     1020

agagccaaag uagaaguuac ucccaauagu ccacgagcgg aagcaacauu gggaggguuu     1080

ggaucucuag gucuagauug cgaaccgaga accggauuag auuucaguga uuuauauuau     1140

uuaacaauga auaauaaaca cugguuaguu cacaaagaau gguuccacga uaucccauua     1200

ccauggcaug cuggagcgga uacgggcaca ccacauugga auaacaaaga agcacuaguu     1260

gaauuuaaag augcacaugc aaaacgucaa acaguaguag uauuaggauc ccaagaaggc     1320

gcuguacaua cagcacuagc aggugcauua gaagcugaaa uggauggugc uaaaggacgc     1380

uuauuuucag gacaucuaaa augucgucua aaaauggaca aauuaagacu uaaaggagua     1440

aguuauagcu uauguacugc agcauuuacu uuuacaaaag uaccagcuga aacauuacac     1500

ggaacaguga caguugaagu acaauaugcu gggacagacg gaccauguaa aguaccagcc     1560

caaauggcag uagauaugca aacauuaacg ccuguagguc guuuaauaac ugcuaacccu     1620

gucauaacgg aaucaacaga aaauucaaaa augauguuag aauuagaucc accuuucgga     1680

gacucauaca uaguaauagg aguuggagac aagaaaauaa cucaccacug gcauagauca     1740

ggaaguacga uugguaaagc auuugaagca acggugagag gagcaaaaag aauggcagua     1800

uuaggggaua cagcguggga uuuuggaagu guaggaggag uuuuuaacuc auuaggaaaa     1860

gguauacacc aaauauuugg ugcagcauuu aaaucucuau uuggagguau gucaugguuu     1920

ucacaaauau uaauagguac acuauuagua ugguugggac uaaauacaaa aaauggauca     1980

auaucucuaa caugcuuagc uuuaggagga guaaugauau uuuuaaguac agcaguguca     2040

gca                                                                   2043


<210>  2690
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short CDS opt

<400>  2690
acuucaauag gaauaaucgg guuacuauua acaaccgcaa uggcagcuga aauaacccga       60

agaggaucag cauacuauau guaccuagau agaucagacg caggaaaagc aauaagcuuu      120

gcaacgacgc ucggaguaaa caaaugucau guccaaauaa uggauuuggg acacaugugu      180

gacgcaacua ugucauacga auguccaaug cuugaugaag gaguagaacc agaugaugua      240

gauuguuggu guaacacaac uucaacaugg guaguauacg gaacuuguca ucauaagaaa      300

ggagaaacca gacgaagccg aaggucaguu agucuaagau aucacuacac aagaaaacua      360

caaaccagau cacaaacaug gcuagaauca agagaauaua aaaaacacuu aauuauggua      420

gagaauugga uauuucguaa uccagguuuu gcaauaguga guguagcaau uacuuggcuu      480

augggaaguu uaacuaguca aaaaguuaua uauuuaguaa ugauaguacu aaucguacca      540

gcauacagca uaucaugcau aggaguuagu aacagagauc uaguugaagg aaugucagga      600

ggaacuuggg uagacguugu auuagaacac ggaggaugug uaacagaaau ggcgcaagac      660

aaaccgacag ucgauauaga auugguaaca augacaguaa gcaauauggc agaaguaaga      720

ucauauuguu augaagcauc acuaucugac auggcaucgg caucacgaug cccaacacaa      780

ggugaaccaa guuuagauaa acaaagugau acacaaagcg uuuguaaaag aacauuaggu      840

gauagagguu ggggaaacgg uuguggaaua uuuggaaaag gaucacucgu aacauguucu      900

aaauuuaccu guuguaaaaa aaugccagga aaauccauuc aacccgaaaa uuuggaauau      960

agaauaaugc ugcccguaca uggaagucaa cauaguggaa ugauaguaaa ugauauagga     1020

caugaaacag augaaaaucg ugcaaaaguu gaaguaacac caaauucacc uagagcagaa     1080

gcaacguuag gaggauuugg aagucuaggc uuagauugug aaccaagaac aggauuagau     1140

uuuuccgauu uauauuaucu aacaaugaau aacaaacacu gguuaguaca uaaagaaugg     1200

uuucaugaua uaccauuacc auggcaugcg ggagcugaca cuggaacacc acauuggaac     1260

aauaaggaag cgcuuguaga auucaaagau gcacacgcaa aaagacaaac aguaguagua     1320

cuaggaucac aagaaggagc aguacauaca gcauuagccg gagcacuaga agcagaaaug     1380

gacggagcaa aagguagacu auucaguggu cauuuaaaau gcagguuaaa aauggauaaa     1440

cuccguuuaa aaggaguuuc uuauaguuua uguacagcag cauuuacauu uacuaaagua     1500

ccugcagaaa cauuacaugg aacaguaacu guagaaguac aaucagccgg aacagaugga     1560

ccuugcaaag uaccugcaca aauggcagua gauaugcaaa cacuaacacc gguaggaaga     1620

cuaauaacag caaaccccgu aauaacugaa ucaacagaaa acucuaaaau gaugcuagaa     1680

cuagauccuc cuuuugguga cucauauauc gugauaggcg uaggagauaa aaaaaucaca     1740

caucacuggc acagaucagg aucuaccaua ggaaaagccu ucgaagcaac aguaagagga     1800

gcaaaaagga uggcaguguu aggcgauaca gcuugggauu uuggcagcgu aggcggagua     1860

uuuaacucac uuggaaaagg aauacaucaa auauuuggcg cagcauucaa aucccuauuu     1920

ggaggaauga guugguuuuc acaaauauua auagguacau uacuuguaug guuagguuua     1980

aauacaaaaa acggcucaau caguuuaacu ugccuagcuu uaggaggagu aaugauauuu     2040

cuaagcacag cagugagcgc c                                               2061


<210>  2691
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short CDS opt

<400>  2691
aguguuggaa uaguaggauu auuacuuaca acagcuaugg cagcagaggu cacaagacga       60

ggaucagcuu acuauaugua uuuagacagg aaugaugcug gugaagcaau aucauucccg      120

accacccugg guaugaacaa auguuacaua caaauuaugg aucuuggaca caugugugac      180

gcaacuaugu cauaugaaug uccaaugcua gaugaaggag uagaaccaga cgauguagac      240

ugcuggugca acacaacaag cacuugggua guauauggca ccugucauca uaaaaaagga      300

gaagcuagaa gaaguagaag agcaguaaca uuaccaagcc auuccacuag aaaauuacaa      360

acaagaucac aaacaugguu agaauccagg gaauauacaa agcaccucau aagagucgaa      420

aauuggauau uuagaaaccc aggauucgcc cuugcagccg cagcaauagc cugguuacuu      480

ggaucgagca caagucaaaa aguuaucuau cucguaauga uauuauuaau agcaccagca      540

uauagcauac gauguauugg cguaucgaau agagauuuug uagaaggaau gagcggagga      600

acauggguag acgucguucu agaacauggu ggaugcguaa caguaauggc acaagauaaa      660

ccuacaguug auauagaauu aguaacaaca acaguaagua auauggcaga aguaaggagc      720

uauugcuacg aagcaucaau cagugauaug gcauccgauu caagaugucc aacacaagga      780

gaagcauacu uagauaaaca aucagauaca caauauguau guaaacguac acuuguagac      840

agaggauggg gaaacggaug uggucuauuc ggaaagggua gccuaguaac augcgcaaaa      900

uucgcaugca guaaaaaaau gacugguaaa agcauacaac cagaaaaucu ugaauauaga      960

auaauguuau caguacaugg aagucaacau ucugggauga uaguuaauga uaccggacau     1020

gaaacugaug aaaacagagc aaaaguugaa auuacuccaa auucaccaag agcagaagca     1080

acauuaggug gcuuuggaag uuugggacuu gauugcgaac cuagaacagg acuagacuuu     1140

agugauuugu auuacuuaac caugaacaau aaacauuggu uaguacauaa agaaugguuc     1200

caugacauuc cauuacccug gcaugcugga gcggauaccg ggacaccaca uuggaacaac     1260

aaagaagcau uaguagaauu uaaagacgca cacgccaaaa gacaaacugu aguaguacug     1320

ggaagccaag aaggagcagu acauacagcg cuagcaggag cauuagaagc agaaauggau     1380

ggugcaaaag gaagauuaag cucaggucau uuaaaaugca gacuaaaaau ggacaaacuu     1440

agauuaaaag guguaaguua uucacuaugu acugcagcuu ucacauuuac uaaaauacca     1500

gcagaaacac uacauggcac aguuacugua gaaguccaau acgcaggaac agauggaccu     1560

uguaaaguuc ccgcacagau ggccguagau augcaaacac uuacuccagu aggccgacuu     1620

auaacagcaa acccuguaau aacagaauca acagaaaauu ccaaaaugau guuggaauua     1680

gaccccccgu uuggugauag uuacauagua auaggagucg gggaaaaaaa aauaacccau     1740

cacuggcauc gauccggcag cacaauagga aaagcauuug aagccacugu aagaggagca     1800

aaaagaaugg cgguauuagg agauacagca ugggauuuug gaagcguagg uggcgcacua     1860

aauuccuuag gaaaagguau acaucaaaua uuuggagcug cauuuaaauc ucuuuuuggg     1920

ggaaugucau gguuuaguca aauuuuaaua ggaacucuau uaaugugguu aggucucaau     1980

acaaaaaacg gaucaauaag uuuaaugugu uuagcuuugg gaggaguccu aauauuuuua     2040

agcacggcug uaagcgca                                                   2058


<210>  2692
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short CDS opt

<400>  2692
acaucaguag guauaguagg acuacuguua acaacagcaa uggcagcaga aguaacuaga       60

agaggaucgg cauauuauau guaucuugac cggaaugaug cgggagaagc uauaagcuuu      120

ccaacuacuu uaggcaugaa uaaauguuau auccaaauaa uggaucuagg acauaugugu      180

gacgcaacaa ugaguuacga auguccaaug uuagacgaag ggguagagcc ugaugacgua      240

gauugcuggu gcaacacgac aaguacaugg gucgucuaug gaacauguca ucauaaaaaa      300

ggagaagcac gaagaaguag gcgagcagua acucuucccu cacacaguac acgaaaauua      360

caaacaagaa gucaaacaug guuagaaucc cgagaguaua caaaacacuu gauaagagua      420

gaaaauugga uuuuuagaaa uccaggauuu gcacuagcag ccgcagcaau cgcauggcua      480

cuuggaagca guacaaguca aaaagucauu uauuuaguca ugauauuauu aauugcaccu      540

gcauauucaa uaagaugcau cggagugucg aauagagauu uuguagaagg aaugagugga      600

ggaacauggg uagauguagu auuagaacau ggaggcugug uaacugucau ggcccaagau      660

aaacccacag uagauauaga auuaguaacg acaacaguau caaauauggc agaaguaaga      720

ucauacuguu augaggcuag uauuucagac auggcaagug auucuagaug ucccacgcaa      780

ggugaagcau aucuagacaa gcaaucagau acacaauaug uauguaaaag gacauuagug      840

gaucgaggcu ggggaaaugg uuguggacuu uuugggaaag guucauuagu cacaugugca      900

aaauuugcgu guucaaaaaa aaugacagga aaaaguaucc aaccagaaaa cuuagaauac      960

agaauaaugc uaucuguaca cggaucucaa cauaguggca ugauaguaaa ugauacggga     1020

caugaaacug acgaaaacag agcaaaagua gaaauaacac caaacucacc uagagccgaa     1080

gcaacauuag gcggauuugg aucucuagga uuagauugcg aaccaagaac uggauuagau     1140

uucucagauu uauauuaucu aacaaugaau aacaaacauu gguugguaca uaaagaaugg     1200

uuucacgaca ucccauuacc auggcaugca ggagccgaua cuggaacacc acauuggaac     1260

aauaaagaag cacuuguuga auuuaaagau gcacacgcaa aacgacaaac uguuguaguu     1320

cuaggaucgc aagaaggugc cguacauaca gcgcuugcag gagcacucga agcagaaaug     1380

gauggcgcaa aaggaagauu aaguucaggg caucuuaaau gcagacuaaa aauggauaaa     1440

uuaagguuaa aaggaguuuc auauaguuua ugcacagcag cauuuacauu uacuaaaaua     1500

ccagcggaaa cauugcacgg aacagugacc guagaagugc aauaugccgg aacagacggu     1560

cccuguaaag uaccagcuca aauggcugua gacaugcaaa cguuaacucc agucggaaga     1620

uuaaucacag cgaacccagu aauaacagaa aguacagaaa auucaaaaau gauguuagaa     1680

uuagacccac cauuugguga uagcuauaua guaauaggag uaggugaaaa aaaaauaacc     1740

caucauuggc auaggucugg aaguacaaua gguaaagcau ucgaagcaac aguaagagga     1800

gcuagaagaa uggcuguauu gggagauacu gcgugggacu uuggaucagu agggggagcc     1860

cuuaauucac uaggaaaagg aauacaucaa auuuuuggag cagcauucaa aucacuauuu     1920

ggagguaugu caugguuuag ccaaauucua auaggaacau uauuaaugug gcuuggauua     1980

aacacaaaaa auggaaguau uucacuaaug ugccuagcac ucgguggagu guuaaucuuu     2040

uuaucaacag caguaucugc g                                               2061


<210>  2693
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short CDS opt

<400>  2693
acuucaguag guauaguagg auuacuuuua acuaccgcga uggcagcaga aguaacaaga       60

agagggucag cuuauuacau guacuuagac agaaaugaug caggagaagc aaucaguuuc      120

ccaacuacau uagguaugaa caaauguuac auccaaauua uggauuuagg acauaugugu      180

gacgcaacaa uguccuacga auguccaaug uuagaugaag gaguugaacc cgaugacguc      240

gacuguuggu gcaauacaac aaguacuugg guagucuaug ggacaugcca ucauaagaaa      300

ggagaagcga gaagaagucg acgagcagua acacuaccau cucauaguac aagaaaauua      360

caaacacgaa gucaaacaug gcuagaaucu agagaauaua caaaacaucu aaucagagua      420

gaaaauugga uguuucgaaa uccuggauuu gcacuagccg cagcagcaau agcauggcua      480

uuaggaucau caacaucaca aaaaguaauu uacuuaguaa ugauuuuguu aauagcuccu      540

gcauauagua uaagaugcau aggaguauca aacagagauu uuguagaagg aaugucagga      600

gguacauggg uagauguagu uuuagaacau ggcggaugug uaacaguuau ggcacaagau      660

aaaccaacug uagauauaga auuaguaaca acuacaguau cuaacauggc ugaaguuaga      720

ucguacuguu acgaagcaag cauuucugac auggcaucag auucuagaug uccuacacaa      780

ggagaagcau aucuugauaa acagucagau acacaauacg uauguaaaag aacauuagua      840

gauaggggau ggggaaaugg augcggucua uuugggaaag gaucguuagu uacaugugca      900

aaauucgcau guaguaagaa aaugacaggc aaaucaauac aacccgagaa ccuugaauau      960

agaauaaugu uaagcguuca uggaucccaa cacagcggaa ugauagucaa cgauaccggu     1020

cacgaaacug augaaaaccg ugcaaaagua gagauaacuc caaacucacc acgugcagaa     1080

gcaacauuag gagguuuugg cucucuagga cucgacugug aacccagaac uggucuagac     1140

uuuucagauc uauauuauuu gacaaugaau aacaaacauu ggcugguuca uaaagaaugg     1200

uuccaugaua uaccauuacc auggcaugcc ggagcagaua caggaacucc gcacuggaac     1260

aauaaagagg cuuuaguaga auuuaaagau gcccaugcaa aacgacaaac aguaguugua     1320

uuaggaaguc aagaaggugc aguucacaca gcucuggcag gagcauuaga ggcagaaaug     1380

gacggcgcua aagguagauu aucauccgga cauuuaaaau guagauuaaa aauggauaaa     1440

uuaagauuaa aagguguaag cuauagccua ugcacugcug cauuuacauu cacaaaaaua     1500

ccagcagaaa cauuacaugg aacaguuacg guugaaguuc aauaugcagg uacugacgga     1560

ccauguaagg uaccugcaca aauggcagug gauaugcaaa cauugacucc cguugguagg     1620

uuaauaacag caaauccagu cauaacugaa ucaacagaaa auucaaaaau gaugcuagaa     1680

uuagauccuc cauuuggaga caguuacaua guaauaggag uaggugaaaa gaaaauaaca     1740

caucauuggc acagaagcgg aaguacuauc ggaaaagccu uugaagccac gguuagaggu     1800

gcaaaaagaa uggcgguauu gggagauaca gcaugggacu ucggcagugu aggaggagcu     1860

uuaaauucau uaggaaaagg aauacaccaa auauucggag cugcauuuaa aagccucuuc     1920

ggagguaugu caugguuuuc ccaaauucua auaggaacac uacuaaugug guuaggacua     1980

aauacuaaaa auggaucaau aucacuaaug ugcuuagcac ucggaggagu acuaaucuuc     2040

cuaucaacag caguaagugc a                                               2061


<210>  2694
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short CDS opt

<400>  2694
acaaguguag guauaguagg acuauuauua acaacagcua uggcagcaga aguaacgcgg       60

agagggucag cguauuauau guaucucgau agaaacgaug ccggagaagc aauaagcuuu      120

ccaacaacau ugggaaugaa uaaauguuau auacaaauaa uggauuuagg acauaugugu      180

gaugcaacca ugucauacga gugcccaaug uuagaugaag guguagaacc agaugauguu      240

gauuguuggu gcaacaugac aaguacaugg guagucuaug gaacauguca ucacaaaaaa      300

ggagaagcaa gaagaucaag aagagcagua acacuacccu cccauucaac gagaaaacuu      360

caaacaagga gucaaacaug guuagaaucc cgagaauaca caaaacaucu aauaagagua      420

gaaaauugga uauuucgaaa uccagguuuu gcucuagcug cagcagcgau agcaugguua      480

cuuggaagcu cuaccucaca aaaaguaaua uauuuaguua ugauucuacu aauagcacca      540

gcauauucaa uaagaugcau aggaguauca aaucgagauu uugucgaagg aaugucagga      600

ggaacauggg uagauguugu ccuagaacau ggagggugug uuacuguaau ggcacaagau      660

aaaccuacag uagacauaga acuuguaaca acaacaguau caaauauggc ugaaguucgu      720

ucauauuguu augaagcuag uauaagcgac auggccagcg auucaagaug uccaacacaa      780

ggagaagcau acuuagauaa acaaagugau acacaauaug uaugcaaacg aacucuuguu      840

gauagaggau gggguaaugg uugcggauua uuuggaaaag gaucacuugu aacaugugca      900

aaauuugcau guaguaagaa aaugacgggc aaaucaauac aacccgaaaa uuuggaauau      960

agaauaaugu uaaguguuca uggaagccaa cacucaggaa ugauaguaaa ugauacagga     1020

caugaaacag augaaaaucg agcaaaagua gaaauaaccc cuaacagccc acgagcagaa     1080

gcaacguuag gaggauuugg aucacuaggc cuagauugug aaccacgaac uggacuugac     1140

uuuagcgauu uauacuaccu gacuaugaac aacaaacacu gguuaguaca uaaagaaugg     1200

uuucaugaca uaccacuucc uuggcaugca ggugcagaua caggaacacc acauuggaau     1260

aauaaagaag cuuuaguaga auuuaaagau gcgcaugcaa aacgccaaac aguuguagua     1320

uuaggcuccc aagaaggagc gguacauacc gcccucgcag gagcauuaga agcagaaaug     1380

gauggagcca aagguagauu aucaucuggc cauuuaaaau guagguuaaa aauggacaaa     1440

uuacgauuaa aagguguaag uuauucauua ugcacagcug cauucacauu cacaaaaauu     1500

ccagcagaaa cauuacacgg aacaguaaca guugaaguuc aauacgcggg aaccgacgga     1560

ccauguaagg uaccagccca aauggccgua gacaugcaaa cauuaacacc aguaggaaga     1620

uuaauaacag cuaauccagu aauaaccgaa agcacagaaa auucaaaaau gauguuagaa     1680

uuagauccuc cauuuggaga uucguauauc gugauugggg uaggagaaaa aaaaauuaca     1740

caccacuggc auagaucagg cucaaccaua ggaaaagcau uugaagcuac uguaagaggu     1800

gcuaaaagaa uggcaguucu cggugacacc gcaugggacu uuggaagugu agguggugca     1860

uuaaauucau uaggaaaagg aauccaccaa auauuuggag cagcauuuaa aucguuauuc     1920

ggaggcaugu caugguuuag ucaaauauua auagguacuu uauuaaugug guuaggacua     1980

aauacaaaaa augguaguau aaguuuaaug uguuuagcuc uaggaggagu auugauauuc     2040

uuaucaacug caguuagcgc u                                               2061


<210>  2695
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short CDS opt

<400>  2695
accagcguag gaauaguugg ucuauuacua acaacagcua uggcagcaga aguaacaaga       60

aggggaucag cauauuauau guauuuagau cgaaaugaug cuggagaagc cauaucuuuu      120

cccacaacac uaggaaugaa caaaugcuau auacaaauua uggauuuagg ucauaugugu      180

gaugcaacaa ugucauacga auguccuaug uuagaugaag gaguagaacc ggaugaugua      240

gauuguuggu guaauacaac cucaacaugg guagucuacg guacuuguca ucauaaaaaa      300

ggugaagcaa gaaggaguag gagagcggua acuuuaccau cgcauucaac aaggaaacuu      360

caaacaagaa gucaaacaug gcuagaaagu agagaauaua caaaacaucu cauaagaguu      420

gaaaacugga uauucagaaa uccuggcuuu gcuuuggcug cagcagcaau agcgugguua      480

cuagggucau caacaucgca aaaaguuaua uaccugguua ugauauuauu aauagcaccg      540

gcauauagua uuagauguau aggaguauca aauagagauu uuguugaagg aaugagcggc      600

ggaacauggg uagacguugu ucuagaacau ggaggaugcg uaacagcuau ggcucaagau      660

aaaccgacag uagacauaga auuaguaaca acuacaguua gcaauauggc ugaagucagg      720

ucauacuguu acgaagccag cauaagugau auggcuagcg acaguagaug ucccacucaa      780

ggagaagcau aucuagauaa acaaagcgau acacaauaug uguguaaacg uacauuagua      840

gacagaggau ggggcaaugg augcggauua uuuggaaaag guagucuagu aacaugugca      900

aaauuugccu gcagcaaaaa aaugacagga aaaaguauac aaccagaaaa cuuagaauac      960

cgaauaaugu uaucaguaca ugguagucaa cacaguggaa uguuaguaaa cgauacaggu     1020

caugaaacag augaaaaucg ugcaaaagua gaaauaacac caaauucgcc aagggcagaa     1080

gcaacucuag gaggauuugg aucacuaggu uuggauugug aaccaaggac aggacuagau     1140

uuuucagauu uauauuaucu aacuaugaau aauaaacauu gguuagcaca uaaagaaugg     1200

uuucacgaua uaccauuacc auggcacgcg ggagcagcaa cuggcacacc acauuggaau     1260

aauaaagaag cauuaguaga auucaaagau gcacacgcaa aaaggcaaac uguaguagua     1320

cuaggcucac aagaaggagc cguacauacc gccuuagcag gagcauuaga agcugaaaug     1380

gauggagcaa aaggaagauu aucuucugga caucuaaaau gucgauuaaa aauggauaaa     1440

cuaagauuga aaggaguaag cuacuccuug uguacagcug cauuuacauu uacaaagauc     1500

ccagcugaaa caguugaugg aacuguaacu guagaaggac aguauggagg aacagauggg     1560

ccuuguaaag uuccagcaca aauggccgua gauaugcaaa cguuaacucc uguuggaaga     1620

cuaauaacag caaauccggu aaucacagaa agcacagaaa auagcaaaau gauguuagaa     1680

uuagacccac cuuuuggaga uucauauaua guaauuggug uaggagaaaa aaaaauaaca     1740

caccacuggc auagaucugg aaguacaaua ggaaaagcau uugaagcaac cguaagggga     1800

gcgaaaagaa uggcaguucu aggcgauaca gccugggacu uuggaagcgu cggaggcgca     1860

uuaaauaguu uaggaaaagg aauacaucaa auuauaggag cugccuucaa aucauuauuc     1920

ggaggcaugu cuugguuuag ucagauauua aucgguacau uacuaaugug guuaggauua     1980

aauacaaaaa acgguagcau aucauuaaug ugucuagcgu uaggaggagu uuuaauuuuu     2040

cuaaguacag caguaucagg g                                               2061


<210>  2696
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short CDS opt

<400>  2696
ggggcagacg cuucuauagg aauaguaagc cuauuauuaa cuacuguuau ggcagcagaa       60

auaacaagaa gggguagugc cuauuauaug uauuuagaca gaagugaugc aggaaaagca      120

auaaguuuug ucacaacuuu aggaguaaau aaaugucacg uucaaauaau ggauuuagga      180

cauaugugcg augcgacaau gaguuaugaa uguccuaugu uagaugaagg cguagaacca      240

gaugacguag auuguuggug uaauaccaca ucaacuuggg uuguauaugg aacuugucau      300

cacaaaaaag gagaagcgag aagaucaagg agagcaguaa cauuaccaag ucauucaaca      360

agaaaacuuc aaacaagaag ccaaacaugg uuagaaagua gagaguacac uaaacaucua      420

auaaaaguag aaaauuggau auuuagaaau ccagguuuug cacuaguugc agucgcuaua      480

gccuggcuac uaggaucauc uacaagccaa aaaguaaucu aucuaguaau gaucuuacua      540

auagcaccag cauauaguau aagauguauu ggaguaagca auagagauuu uguagaagga      600

augagcggag gaacuugggu ugacguagua uuagaacaug gcgguugcgu uacagucaug      660

gcacaagaua aaccaacagu agauauugaa cuagugacaa caacagugag caauauggca      720

gaaguaagau cguauuguua ugaagcauca auaucugaua uggcuucuga uagcagaugc      780

cccacacaag gugaagcaua uuuagacaaa caaagugaca cacaauaugu auguaaaaga      840

acauuaguag acagaggaug gggaaacgga uguggauugu uuggaaaagg uaguuuaguu      900

acaugugcaa aauucacuug uucaaaaaaa augacaggaa aaucuaucca accagaaaau      960

uuagaauaca gaauuaugcu aaguguucau ggaagucaac acucgggaau gauagucaau     1020

gacgaaaaca gggccaaagu ugaaguuacg ccaaacucac cuagagcaga agccacacua     1080

ggaggauuug gaucacuagg acuugacugu gaacccagaa cgggacuaga uuuuagugau     1140

uuauauuauc uuacaaugaa uaacaaacau uggcuaguac acaaagaaug guuucaugac     1200

auaccacuac ccuggcacag uggugcagau accgaaacac cacauuggaa caauaaagaa     1260

gcacuaguag aauucaaaga ugcacaugca aagagacaga ccgugguugu acuagggagc     1320

caggaaggag caguacacac ggcauuagcg ggagcauuag aagcagaaau ggacggagcg     1380

aaaggaagau uaucaagcgg acaucuaaaa uguagauuaa aaauggauaa auugagauua     1440

aaaggaguuu cauacaguuu auguaccgcu gcauuuacuu uuacuaaagu ucccgcugaa     1500

acgcuacacg gaacaguaac aguagaaguu caauaugccg gaagagacgg ucccuguaaa     1560

guacccgcac aaauggccgu agauaugcaa acacuaacac cggugggaag acuaauaacc     1620

gccaacccag uuauuacaga aucaacagag aauucaaaaa ugauguuaga auuagauccc     1680

ccauuuggug auucuuauau aguaauagga guaggugaua aaaaaauaac ccaccacugg     1740

cauaggagcg gaucaauaau aggcaaagca uucgaagcaa caguccgagg agcaaaaaga     1800

auggcaguac uuggugauac cgcaugggac uuuggaagug uaggaggagu auuuaauagu     1860

cuagguaaag gaauucauca aauuuuugga gcggcuuuua agucauuauu cggaggaaug     1920

uccugguuca gucaaauccu aauaggaacc uugcuaguau gguuaggauu aaauacaaaa     1980

aauggcucaa uuucauuaac augucuagca cuuggcggag uaaugauauu uuuauccacu     2040

gcaguaucgg ca                                                         2052


<210>  2697
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short CDS opt

<400>  2697
ggagccgaca cuaguauagg aauaauagga uuauuauuaa cuacagcaau ggcggcugaa       60

auaacucgaa gaggaucagc auauuauaug uauuuagaua gaucagaugc uggaaaagca      120

auaaguuuug caacaacaau gggaguaaac aaaugccaug uacagauuau ggaucuaggu      180

cacaugugug augcuacaau gaguuaugaa ugcccaaugu uagaugaggg aguagaacca      240

gaugaugugg auuguuggug uaauacaacg ucaacauggg uuguauaugg aacaugucac      300

cacaaaaaag gagaagcaag gccgucaaga cgagcaguaa cauuaccuag ucauaguacc      360

agaaaacuac aaacuagaag ccaaacaugg uuagaaagua gagaauauac aaaacacuua      420

auaaaagugg aaaauuggau acuaaggaau ccaggcuuua cuguagcggc auuagcuaug      480

gcauggcuac uugggaauuc aacaucacaa cgaguaauau auuuaguaau gauauuacua      540

auagcgccug cauauuccau aagaugcauu ggaguaagca accgcgacuu uguagaggga      600

augucaggcg gaacuugggu ugauguagua uuagaacacg guggaugugu aacuguaaug      660

gcacaagaca aaccuacagu agacauugaa uuaguuacga caacaguaag caauauggca      720

gaaguacguu ccuauuguua ugaagcuagu auaucagaua uggcaagcga uucuagaugu      780

ccuacacagg gagaagcaua uuuagacaaa caaagugaua cacaauacgu augcaaacgc      840

acccuaguag aucgaggaug gggaaacggg ugcggauuau uugguaaagg aucauugguu      900

acuugcgcga aauuuacaug uaguaaaaaa augacaggaa aaucaauuca accugaaaau      960

uuagaauauc gaauaaugcu gucaguacau ggaucacaac auaguggcau gauagucaau     1020

gauauaggac augaaacaga ugaaaacagg gccaaaguag aagucacccc caauuccccc     1080

agagcagaag cgacauuagg cggcuuugga aguuuaggau uagauugcga acccagaaca     1140

ggauuagauu uuucugauuu auauuaucua acaaugaaca acaaacacug guuaguacac     1200

aaagaauggu uucacgacau accccuaccg uggcaugcag gagcagauac cggaacacca     1260

cauuggaaua auaaagaagc auuaguagaa uuuaaagaug cucacgcaaa aagacaaaca     1320

guuguaguau uaggaucuca agaaggugcu guacauacag cacuugcagg agcauuagaa     1380

gcagaaaugg auggagcaaa aggaagauua uuuuccggac accuuaaaug cagaauaaaa     1440

auggacaagc uaagauuaaa gggaguaagu uauucacuau guacugcagc uuuuacauuu     1500

aguaaaguac cagcagaaac uuuacauggu accguaacug uugaaguuca auaugcagga     1560

agggaugggc caugcaaagu accugcccaa auggcaguag auaugcaaac uuuaacgccu     1620

guagguagau uaauuaccgc aaauccuauu auuacagaau caacugaaaa uagccgcaug     1680

auguuagaac uagauccgcc auuuggugau agcuauauag uuauaggagu aggagauaaa     1740

aaaauaacac aucauuggca uagaagugga ucaaccauag gcaaagcauu ugaagcaaca     1800

guaagaggag cgaaaagaau ggcaguacua ggcgauacug caugggacuu uggaucagua     1860

gguggaguau ucaauucauu aggaaaagga guacaucaag ucuuuggagc ugcuuucaaa     1920

aguuuauuug gagggauguc augguuuagc caaauauuaa uugggacuuu auuaguuugg     1980

cugggauuaa auacaaaaaa uggaaguaua ucauuagcuu gucuugcacu aggaggagca     2040

augauauucc uaagcacagc uguaucagca                                      2070


<210>  2698
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE CDS opt

<400>  2698
ucacaaaaag uaauauauuu aguaaugaua cuauuaauag caccagcuua uagcauaaga       60

uguauaggag uuucaaacag ggauuucguc gaaggaauga gugguggaac auggguagau      120

auaguucuag aacauggagg uuguguaaca gugauggcac aagauaaacc aacuguagau      180

auagaauuag uuacuacaac cguaucaaau auggcagaag uuagaucaua uuguuaugaa      240

gcaucaauaa gugauauggc cucagauuca agauguccaa cacaggggga agccuacuua      300

gauaaacaau cagauacuca auauguaugu aaaagaacau uaguagauag agguugggga      360

aauggaugug gauuauuugg aaaagguagu uuaguaacgu gugcaaaauu cgcauguucu      420

aaaaaaauga caggaaaaag uauacaaccu gaaaaccuag aauauagaau aaugcuauca      480

guacauggua gucaacauuc gggaaugaua guaaaugaua caggacauga aacagacgaa      540

aauagagcaa aaguugaaau aaccccaaau aguccaagag cagaagcaac auuaggagga      600

uuuggaucau uaggacuaga uugcgaacca agaacaggau uagauuuuag ugauuuauau      660

uaucuaacaa ugaauaauaa acacugguua guacauaaag aaugguucca ugauauacca      720

cuaccauggc acgcaggugc agauacagga acaccacauu ggaauaauaa agaagcauua      780

guugaauuca aagacgccca ugcaaaaaga caaacaguag uaguacuugg uagucaagaa      840

ggugcuguuc acacagcauu ggcaggagca uuagaagcag aaauggaugg agcuaaagga      900

agauuaucau caggacauuu aaaaugcaga uuaaaaaugg auaaacuaag acuaaaagga      960

guaaguuaua gucuaugcac ggcggcauuu acauucacua aaauaccagc ggaaacacua     1020

caugggacag uaacaguaga aguacaauau gcaggaacag auggaccuug uaaaguucca     1080

gcucaaaugg caguagauau gcaaacauua acaccgguag guagauuaau aacagcuaac     1140

ccaguaauaa cagaaaguac ugaaaauuca aaaaugaugc uagaacuaga uccaccauuu     1200

ggagacucau auauaguaau aggaguagga gaaaaaaaaa uaacucauca uuggcacaga     1260

ucaggaagua caauaggaaa ggcauucgaa gcaacaguaa gaggagcuaa aagaauggca     1320

guuuuaggag auacagcaug ggauuuugga agc                                  1353


<210>  2699
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE CDS opt

<400>  2699
ucacaaaaag uaauauauuu aguaaugaua cuacucauag caccagcuua uaguauaaga       60

ugcauuggag uaucaaaucg agauuuugua gaagguaugu caggaggaac auggguagau      120

guaguacuag aacauggugg auguguaacu guaauggcac aagacaaacc aacaguagac      180

auagaauugg uaacaacaac aguaaguaau auggcagaag uaagaucaua uuguuaugaa      240

gcaagcauau cagauauggc aagugacagc agguguccaa cucaagguga agcauaccua      300

gauaaacaau cagauacaca auauguuugu aaaagaacau uagucgacag agguugggga      360

aaugguugcg gacuauucgg aaaaggaagc uuaguaacau gugcaaaauu ugcauguagc      420

aaaaaaauga cagguaaauc aauucaacca gaaaauuuag aauacagaau aaugcuuagu      480

guacacgguu cccaacauag uggaaugauc guaaaugaca ccggacauga aacugaugaa      540

aaucgugcaa aaguagaaau aacaccaaau uccccaagag cugaagcaac acuaggagga      600

uucgguucau uaggauuaga uugugaaccu agaacaggau uagauuuuuc agaucuauau      660

uaucuaacga ugaauaauaa acauugguua guacauaaag aaugguuuca ugauauacca      720

uuacccuggc augcuggagc agauacaggg acaccacauu ggaauaauaa agaagcguua      780

guugaauuua aagacgcaca ugcaaaaaga caaacaguag uaguacuugg aucacaagaa      840

ggcgcaguac auacagcacu agcaggagca cuggaagcug aaauggaugg ugcaaagggu      900

agacuaucaa gcggacaucu aaaauguaga cuaaaaaugg auaaacuaag acuaaaagga      960

guuuccuaua guuuguguac agcagcauuu acuuuuacaa aaauaccagc agaaacauua     1020

cauggaacag uaacaguaga aguucaauau gcaggaacgg auggaccaug uaaaguacca     1080

gcacaaaugg caguagacau gcaaacauua acaccaguag gaagauuaau aaccgcuaau     1140

ccaguaauaa cagaaucaac agaaaauuca aaaaugaugc uugaacuuga uccaccauuu     1200

ggagauucau acauaguaau aggaguagga gaaaaaaaaa uaacacauca cuggcacaga     1260

aguggaucaa caauaggaaa agcauuugaa gcaacgguaa gaggagcaaa aagaauggca     1320

guauuaggag auacugccug ggauuuugga agc                                  1353


<210>  2700
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE CDS opt

<400>  2700
ucucaaaaag uaauauauuu aguuaugaua uuauuaauag caccagcaua uucaauaaga       60

uguauaggag uaucaaauag agacuuugua gaaggaauga guggaggaac auggguagau      120

guaguacuag aacauggagg auguguaaca guuauggcac aagauaaacc aacgguagac      180

auagaauuag uaacaacuac aguaucaaau auggcagaag uaagaagcua cuguuaugaa      240

gcaucaauua gugacauggc aagugacagc agauguccaa cacaaggaga agcauauuua      300

gauaaacaaa gugauacaca auacguaugc aaaagaacau uaguagacag aggaugggga      360

aauggaugug gauuguuugg caaaggaagu uuaguaacau gugcgaaauu uacuuguagu      420

aagaaaauga cagggaaauc aauacaacca gaaaauuuag aauauagaau aaugcuaagu      480

guacaugguu cacaacacuc agggaugauu gguuacgaaa cggaugaaga uagagcaaaa      540

guagaaguua caccaaauag ccccagagca gaagcaacgu uagguggcuu uggcaguuua      600

ggauuagauu gugaaccaag aacaggauua gacuuuagug aucuauauua uuuaacaaug      660

aauaauaaac auugguuagu acacaaagaa ugguuccaug auauaccucu accauggcau      720

gcaggagcag auacaggcac accgcacugg aauaauaaag aagcauuagu agaauuuaaa      780

gaugcacaug caaaacgaca aaccguaguc guauuaggaa gucaagaagg ggcaguucac      840

acagcauuag caggagcucu ugaagcagaa auggauggag caaaaggaag auuauucuca      900

ggacacuuaa aauguagacu aaaaauggau aaauugagac ucaaaggugu aucauauagu      960

cuguguacag cagcauucac auuuacaaaa guaccugcag aaacauuaca ugggacagua     1020

acaguugaag uacaauaugc agguacugau ggaccaugua aaaucccugu acaaauggca     1080

guggauaugc aaacucuaac accaguagga agacuaauaa cagcuaaucc aguaauaaca     1140

gaaagcacag aaaauagcaa aaugauguua gaauuagauc cacccuucgg agauaguuac     1200

auaguaauag gaguuggaga uaagaaaaua acacaccacu ggcauagauc aggcaguaca     1260

auaggaaaag cauuugaagc uacagugaga ggugcuaaac gaauggcugu auuaggagau     1320

acagcuuggg auuuuggaag u                                               1341


<210>  2701
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE CDS opt

<400>  2701
agccaaaaag uuauauaucu aguuaugaua cuauuaauag ccccagcaua uaguauaagg       60

uguauagggg ugaguaacag agauuucgua gaaggaaugu caggaggaac auggguagau      120

guaguacuag aacacggagg auguguuaca guaauggcac aagauaaacc aacaguagau      180

auagagcuag uaacgacaac aguaaguaau auggcagaag uucgaucuua uuguuaugaa      240

gcaaguauaa gugauauggc aagugauuca cguuguccaa cacaaggaga agcauauuua      300

gauaaacaaa gugauacuca auauguuugc aaaagaacau uaguagauag aggaugggga      360

aacgggugug guuuauuugg uaaaggaagu uuaguaacau gcgcaaaauu ugcauguagc      420

aagaaaauga cuggaaaaag uauacaacca gaaaauuuag aauauagaau aaugcuaagc      480

guacacggua gucaacauag ugguaugauu gucaaugaua cuggacauga aacagacgaa      540

aauagagcua aaguagaaau aacaccaaau agcccacgag cagaagcuac acucggagga      600

uuugguucuu uaggauugga uugugaaccu cgaaccggac uagauuuuag cgaucucuac      660

uacuuaacaa ugaauaauaa acauuggcua guacauaaag aaugguuuca ugauauccca      720

uuaccauggc augcaggagc agauacagga acaccacauu ggaacaauaa agaagcauug      780

guagaauuua aagacgcaca ugcaaaaaga caaacuguag uaguacuagg aucacaagag      840

ggugcuguac auacagcgcu agcaggcgca cuagaagcag aaauggaugg ugcaaaaggg      900

cgauuaaguu caggacaucu aaaauguaga uuaaaaaugg auaaauuaag acuaaaagga      960

guaucauauu cauuauguac ugcugcauuu acauuuacaa aaauaccggc agaaacauua     1020

cauggaacag uaacaguaga aguacaauau gcaggaacag acggaccaug uaaaguccca     1080

gcacaaaugg caguugauau gcaaacauua acaccuguag gaagauuaau aacagcaaau     1140

ccaguaauaa cagaaucaac agaaaacuca aaaaugaugu uagaauuaga uccuccauuu     1200

ggugacagcu auauaguaau agguguagga gaaaagaaaa uuacacacca uuggcauaga     1260

agcggaucaa ccauaggaaa agcauuugag gcaacaguaa gaggagcaaa aagaauggca     1320

guauugggag acacagcaug ggauuucgga agu                                  1353


<210>  2702
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE CDS opt

<400>  2702
agccaaaaag uaauauauuu aguaaugaua uuacuuauag cuccagcuua uaguauaaga       60

uguauagggg ucucaaauag agacuuuguu gaaggaauga guggaggaac auggguagac      120

guaguuuuag aacauggagg auguguaacu guaauggcac aagauaaacc gacaguagau      180

auagaauuag uaacaacaac cguaaguaau auggcagaag uaagaagcua uugcuaugaa      240

gcaagcauca gugauauggc aagugauagu agauguccaa cacaaggaga agcauaccua      300

gacaaacaaa gugacacaca auauguaugu aaaagaacac uaguagauag aggaugggga      360

aacggaugcg gauuauuugg uaaagguuca uuaguaacau gugcaaaauu uacauguagc      420

aaaaaaauga caggaaaaag cauucaacca gaaaaucuag aauacagaau caugcuaucu      480

guacauggau cacaacacag ugguaugaua guuaaugaua cuggauauga aacagaugaa      540

aauagggcaa aaguggaagu aacuccaaau agcccaagag cagaagcuac auuaggagga      600

uucggaaguu uaggacuaga uugcgaacca agaacaggac uagacuuuuc agaucuauau      660

uauuuaacua ugaauaauaa acauugguua guacacaaag aaugguuuca ugauauacca      720

uuaccauggc acgcaggagc agauacugga acaccccauu ggaauaauaa agaagcacua      780

guagaauuca aagaugcuca ugcaaaaaga caaacuguag uaguauuagg cucacaagaa      840

ggagccgugc auaccgcauu agcuggagcu cuagaagcug aaauggaugg agcaaaaggg      900

aaauuauuca guggacacuu aaaaugucga cuaaaaaugg auaaguugag gcuaaaaggu      960

guaaguuauu cauuauguac agcagcauuc acauuuacua aaguaccagc agaaacauug     1020

cauggaacgg uaacagugga aguccaauau gcaggaacgg auggaccaug caaaauacca     1080

guacagaugg caguagauau gcagacauua acaccaguag gaagacuaau aacagcgaau     1140

ccaguaauaa cagaaaguac agaaaauagu aaaaugaugu uggaauuaga uccccccuuu     1200

ggagauucau acauaguaau aggaguagga gauaaaaaaa uaacucacca uuggcauagg     1260

aguggaucaa caaucggaaa agcauuugaa gcaacuguaa gaggagcaaa aaggauggca     1320

guacuaggag auacagcaug ggauuuugga uca                                  1353


<210>  2703
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE CDS opt

<400>  2703
agucaaaaag ucauauauuu aguaaugaua uuauuaauug caccugcaua uaguauaaga       60

uguauaggag uaaguaauag agacuuugua gaaggaauga gugguggaac auggguagac      120

guagucuuag aacauggagg auguguaacu guaauggcac aagacaaacc aacaguugau      180

auagaauuag uuaccacaac aguaaguaau auggcagaag uaaggaguua uuguuaugaa      240

gcaaguauca gugacauggc aagugauagu cgauguccaa cacaaggaga agcauaucua      300

gauaaacaau cagauacaca auauguaugu aaaagaacau uaguagauag aggauggggu      360

aacgguugug gacuauuugg uaaaggaagc uuaguaacuu gugcaaaauu cacauguuca      420

aaaaaaauga caggaaaauc aauacaacca gaaaaucugg aauacagaau aauguuauca      480

guacauggaa gucaacauag uggaaugaca guaaacgaua uaggauauga aacagaugaa      540

aauagagcaa aaguagaagu aacuccaaau agcccaaggg cagaagcaac acuaggaggu      600

uuuggaucac ugggauuaga uugcgaacca agaacuggac ucgacuucag cgauuuauac      660

uauuuaacua ugaauaauaa acauugguua guucauaaag aaugguuuca cgacauccca      720

uuaccauggc acgcaggagc ggauacaggu acaccacacu ggaacaauaa agaggccuua      780

guagaauuua aagaugcaca ugcaaaaagg caaacuguag uaguauuggg aagucaagag      840

ggagcaguac auacagcacu agcaggugca uuagaagcag aaauggacgg agcaaaaggu      900

aaacuauuuu caggacaucu aaaaugcaga uuaaaaaugg acaaauuacg uuuaaaagga      960

guaaguuacu cacuuuguac agcggcuuuu acauucacaa aaguaccagc agaaacauua     1020

cauggcacag uuacaguaga aguacaauau gcaggaacag acggaccaug uaaaauacca     1080

guacaaaugg caguagacau gcaaacacua accccgguag gaagacuuau aacagcaaau     1140

ccaguaauaa cagaaaguac ugaaaauuca aaaaugaugc uagaauuaga uccuccauuu     1200

ggagauucau acauaguaau agguguaggg gauaaaaaaa uuacacacca uuggcauaga     1260

uccggaagca caauaggaaa agcauuugaa gcaacgguaa gaggagcuaa aagaauggcu     1320

guacuaggag auacagccug ggauuuugga uca                                  1353


<210>  2704
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE CDS opt

<400>  2704
ucgcaaaaag uuauauaucu aguaaugaua uuauuaauag caccagcuua uaguauaaga       60

uguauaggag uaucaaacag agauuuugua gaaggaauga gcggagggac auggguagac      120

guaguauuag aacauggagg uuguguaaca guaauggcac aagauaaacc aacggucgau      180

auagaauugg uaacgaccac gguaucaaau auggcagaag uaagaaguua uuguuaugaa      240

gcauccauaa gcgauauggc aagcgauagu agauguccaa cacaaggaga agcauaucuu      300

gauaaacaau cagauaccca auauguaugu aaaagaacau uaguagauag aggaugggga      360

aaugguugug gauuguucgg aaaaggaucc uuaguaacau gcgcaaaauu uacauguagc      420

aaaaaaauga cuggaaaaag uauacaaccu gaaaaucuag aauauagaau aauguuaucu      480

guacauggaa gucaacauuc aggaaugauu guaaaugacg aaaauagagc caaaguagaa      540

gugacaccaa auagcccaag agcagaagca acauuaggag guuuugguag cuuaggauua      600

gacugugaac cacgaacagg gcuggacuuu ucagacuuau auuaucuaac uaugaauaau      660

aaacauuggc ucguacauaa agaaugguuu cacgauauac cacuaccaug gcaugcagga      720

gcagauacag gcacaccaca uuggaauaau aaagaagcau uaguagaauu caaagacgca      780

cacgcaaaaa gacaaacagu aguaguacua ggaucacaag aaggagcagu acauacagcu      840

uuagcaggag cucuagaagc agaaauggau ggagcaaaag guagacuauu cagcggacau      900

uuaaaaugua gauuaaaaau ggauaaacua agguuaaaag gaguaaguua uaguuuaugu      960

acagcagcau uuacauuuac gaaaguacca gcagaaacau ugcacggaac agugacaguu     1020

gaaguacaau augcgggaac agacggacca ugcaaaguac cagcacagau ggccguagau     1080

augcaaacau uaacaccagu aggucgacua auaacugcaa auccaguuau uacagaaucu     1140

acagaaaaua gcaaaaugau gcuagaguua gauccuccau ucggagacag uuauauagua     1200

auaggaguug gagauaaaaa aauaacacau cauuggcaua gaaguggaag uacaauagga     1260

aaagcauuug aagcaacagu aagaggagcu aaaagaaugg cuguuuuagg agauacagca     1320

ugggacuuug gaagu                                                      1335


<210>  2705
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE CDS opt

<400>  2705
ucacaaaaag uaauauacuu aguuaugaua cuauuaauag caccugcuua cagcauacga       60

ugcauuggug uaagcaaucg ugacuuugua gagggaauga gcgguggaac auggguagac      120

guaguauuag aacacggagg auguguaaca guaauggcgc aagauaaacc aaccguagau      180

auagaauuag uuacaacaac aguaucaaau auggcagaag uaagaucaua uuguuaugaa      240

gcaagcauau cagauauggc aagugacagu cgaugcccaa cacaaggaga agcauauuua      300

gauaaacaaa gcgauacaca auacguaugu aaaagaacau uaguagauag gggauggggc      360

aacgguugcg gauuauucgg caaaggaagc cuaguuacau gugccaaauu uacauguagu      420

aaaaaaauga cuggaaaauc aauacaaccu gaaaauuuag aauauagaau uaugcuaagu      480

guacacggaa gccaacacuc aggaaugauu ggauacgaaa cagaugaaaa cagggcuaaa      540

guagaaguca caccaaauuc accaagagca gaagcaacac uuggaggcuu cgguucgcua      600

ggacucgauu gugaaccaag aacaggacua gacuuuucug acuuauauua ucuaacaaug      660

aauaauaaac auuggcuagu acauaaagaa ugguuccacg auauaccauu gccauggcac      720

gcaggugcag auacaggaac accacauugg aauaacaaag aagcauuagu agaauuuaaa      780

gacgcccaug cuaaaagaca aacaguagua guacuaggaa gucaagaggg ugcaguccau      840

acagcauuag caggagcacu agaagcagaa auggaugggg cuaaagguaa auuauuuagu      900

ggacauuuaa aauguagauu aaaaauggac aaauuaagau uaaaaggagu aaguuacagc      960

uuauguacag cagccuucac auuuacaaaa guaccagcag aaacauuaca uggaacaguu     1020

acaguagaag uacaauaugc aggaacagau ggaccaugua aaauaccagu ccaaauggca     1080

guagauaugc aaacauuaac accaguagga agauuaauaa cagcaaaucc uguaauaaca     1140

gaaaguacag aaaauagcaa aaugauguua gaacuagauc caccauuugg ugacaguuau     1200

auagugauag gaguuggaga caaaaaaaua acacaucauu ggcaucgauc aggaaguaca     1260

auuggaaaag cauucgaagc aacuguaaga ggagcaaaaa gaauggcugu cuuaggggac     1320

acagcauggg auuucggcag u                                               1341


<210>  2706
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE CDS opt

<400>  2706
ucucaaaaag uaauauauuu aguuaugaua cuauugauag cucccgcaua uagucuaaga       60

uguauaggag uaagcaauag agauuuugua gaaggaauga gcgguggaac auggguagau      120

guaguauuag aacauggagg auguguaaca guuauggcac aagauaaacc aacaguagau      180

auagaauuag uaacaacaac aguaucaaau auggcagaag uaagaucaua uuguuaugaa      240

gcaaguauaa gcgauauggc uucagauagu agaugcccaa cccaaggaga agcauaccua      300

gauaaacaaa gugauacaca auacguaugu aaaagaacgu uaguagauag aggaugggga      360

aauggaugcg gauuauuugg aaaaggaagu cuaguaacau gugcuaaauu ugcauguuca      420

aagaaaauga caggaaaauc aauucaacca gaaaauuuag aauauagaau aauguuaagc      480

guacaugguu cacaacauag uggaaugauc guaaaugaua caggacauga aacagaugaa      540

aaucgagcua aaguagaaau aacaccuaac aguccaagag cagaagcaac auuaggagga      600

uuuggaaguu uaggauugga uugcgaacca agaacagguu uagauuucuc agauuuauau      660

uaucuaacaa ugaauaacaa acauugguug guacauaaag aaugguuuca ugauauaccu      720

cuaccuuggc augcaggagc agacacggga acaccacacu ggaauaauaa agaagcauua      780

guagaauuua aagaugcuca ugcaaaaaga caaacuguag uaguauuagg aagucaagaa      840

ggagcaguuc auacagccuu agcaggagca uuagaagcag aaauggaugg agcuaaagga      900

agauuaucaa guggacauuu gaaaugcaga uugaaaaugg auaaauuaag auuaaaagga      960

guaucauaua gucuauguac agcagcauuu acauuuacaa aaauaccagc ggaaacacua     1020

cauggcacag uuacugucga aguacaauau gcaggaacag acgguccaug uaaaguacca     1080

gcacaaaugg cagucgauau gcaaacauua acaccagugg gaagauuaau aacagcaaau     1140

ccaguaauaa cugaaucaac ugaaaauuca aaaaugaugc uagaauuaga uccacccuuc     1200

ggugauucgu acauaguaau aggaguuggg gaaaagaaaa uaacacauca uuggcauaga     1260

ucaggaagua caauuggaaa agcauucgaa gcuacuguua gaggcgcuaa aagaauggca     1320

guauugggag auacagcaug ggauuuugga agu                                  1353


<210>  2707
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE CDS opt

<400>  2707
agcaaaaaag uaauauaccu aguaaugaua uuacuaauag caccagcaua caguauacga       60

uguauaggag uaaguaacag agauuuuguu gaaggaaugu caggaggaac auggguugau      120

guaguauuag aacacggcgg auguguaaca guaauggcac aagauaaacc aacaguugau      180

auagaauuag uaaccacaac aguuagcaau auggcugaag uaagaucaua uuguuaugaa      240

gcaucaauau cagauauggc aagugauuca agauguccaa cacaagguga agcauaucua      300

gacaaacaau cagauacaca auacguaugu aaaagaacac uagucgauag aggaugggga      360

aauggaugcg guuuauucgg aaaaggguca cuaguuacau gugccaaauu ugcaugcagc      420

aaaaaaauga cuggaaaauc aauucaacca gaaaaucuug aauauagaau aauguuauca      480

guacaugguu cacaacauag cggaaugauu guuaacgaca ccggacauga aacagaugaa      540

aaucgagcaa aaguagaaau aacuccaaac ucaccaagag cugaagcaac auugggagga      600

uucggaucau uaggacuaga cugugaacca agaaccggau uagauuuuuc agacuuauau      660

uauuuaacaa ugaauaacaa acauugguua guacacaaag aaugguuuca cgacauacca      720

cuaccauggc acgcaggagc ugauacaggu acaccacauu ggaauaacaa agaagcucuc      780

guagaauuua aagaugcaca ugcaaaacga caaacuguag ucguauuagg aucucaagaa      840

ggagcuguac auacagcacu agcaggagca uuagaagcag aaauggaugg agcuaaagga      900

agauuaagcu caggccaucu caaaugccga uuaaaaaugg auaaauuaag auuaaaagga      960

guaucauauu cauuauguac agcagcauuc acauuuacaa aaauuccagc ugaaacauua     1020

caugguacug uaacaguaga aguacaauau gccgguacag augguccaug uaagguaccu     1080

gcacaaaugg caguagauau gcaaacauua acaccaguag gaagacuaau aacagcaaau     1140

ccuguaauaa cagaaaguac agaaaauuca aaaaugaugc uagaauuaga cccaccguuc     1200

ggcgauaguu auauuguaau aggaguagga gaaaaaaaaa uaacucacca uuggcauaga     1260

ucaggcucaa caauaggaaa agcauuugaa gcaacaguaa gaggagcuaa aagaauggcu     1320

guucuaggag acacagcaug ggauuuuggu uca                                  1353


<210>  2708
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE CDS opt

<400>  2708
agccaaaaag uaaucuauuu agucaugaua uuauugauag ccccagcaua cucuauaaga       60

uguauaggag uaaguaauag agacuucguc gaaggaauga guggaggaac auggguagau      120

guaguucuag aacauggagg auguguaaca guaauggcuc aagacaaacc cacaguagau      180

auagaauuag uaacaacaac aguaagcaau auggcagaag uacgaaguua uugcuaugaa      240

gcaucaauau cagauauggc aucagauuca agauguccaa cccaaggaga agcuuaucuc      300

gauaaacaau cagauacaca auauguaugu aaaagaacuc uaguagauag gggaugggga      360

aaugguugcg gucuauuugg aaaaggaagu uuagucacau gugcaaaauu ugcaugcagu      420

aaaaaaauga caggaaaauc uauacaacca gagaacuuag aauauagaau aauguugagu      480

guacacggua gucaacauag ugguaugaua gucaaugaua caggacauga aacagaugaa      540

aauagagcaa agguagaaau aacgccaaau ucaccaagag cagaagcaac auuaggagga      600

uuuggaagcu uaggacuaga cugugaacca cgaacgggau uagauuuuag ugaccuauau      660

uauuuaacaa ugaauaacaa acauuggcug guucauaaag aaugguuuca cgauauacca      720

cuaccauggc augcuggagc agauacaggu acaccacacu ggaacaauaa agaagcauua      780

guagaauuua aagacgcuca ugcaaaaaga caaacgguag uagucuuagg aucacaagaa      840

ggagcaguac acacagcauu agcgggagca cuagaagcag aaauggaugg ugcaaaagga      900

agauuaagua guggacaucu aaaauguaga uuaaaaaugg auaaauuaag guuaaaagga      960

guaaguuauu cauuauguac ggcggcauuu acauuuacaa aaauaccugc agaaacguua     1020

cauggaacag uuacagucga aguucaauau gcuggaacag auggacccug uaaaguucca     1080

gcacaaaugg cgguagauau gcaaacauua acaccaguug gaagauuaau cacagcaaau     1140

ccaguaauaa cagaaaguac agaaaauagu aaaaugaugc uugaauuaga uccaccauuc     1200

ggagauagcu auauaguaau uggaguaggu gaaaaaaaaa uaacgcauca uugguauaga     1260

ucaggaucaa caauaggaaa agcauuugaa gcaacuguaa ggggagcaaa acguauggca     1320

guauugggag auacagccug ggacuuugga agu                                  1353


<210>  2709
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE CDS opt

<400>  2709
ucacaaaaag uaauauaucu aguaaugaua uuacuuauag cuccugcaua uaguauacga       60

ugcauagggg ugucaaauag agauuuugua gaaggaauga gugggggaac auggguagau      120

guaguacuag aacacggagg uuguguaaca gucauggcac aagauaaacc gacuguggau      180

auagaacuug uaacaacuac aguaucaaau auggcagaag uaagaaguua uugcuacgaa      240

gcaaguauaa gcgacauggc aagcgauucc agguguccaa cacaaggaga agcauaccua      300

gauaaacaaa gcgauacuca auauguaugu aaaagaacgc ucguagauag aggcugggga      360

aauggaugug gauuauuugg uaaagggagu uuaguuacau gugcuaaauu ugcaugcagu      420

aagaaaauga caggaaaaag uauacaacca gaaaauuuag aauaucgaau aauguuguca      480

guucauggaa gucaacauuc aggaaugaua guaaaugaua caggacauga aacagaugaa      540

aauagagcga aaguugaaau aacaccaaau ucaccaagag cagaagcaac acuaggggga      600

uuugggucac uaggacuaga uugugaacca agaacaggau uagauuuuuc agaucucuac      660

uaccuaacaa ugaacaauaa acacugguua guacauaaag aaugguuuca cgacauccca      720

uuaccauggc augcaggagc agauacagga acgccacauu ggaauaacaa agaagcauua      780

guagaauuua aagaugccca ugcuaaaaga caaacaguug uaguacuugg uucacaagaa      840

ggugcaguac acacagcauu agcaggagca uuagaagcag aaauggaugg agcaaaagga      900

aaacuaagcu cuggacaucu aaaaugcaga cuaaaaaugg auaaauuaag auuaaaagga      960

guaagcuaua gucuaugcac agcagcauuc acuuucacaa aaauaccagc agaaacauua     1020

cacggaacag uaacgguaga aguacaauau gcaggaacug acggaccaug uaaagugcca     1080

gcacaaaugg caguagauau gcagacauua acaccaguag guagauuaau aacagcaaac     1140

ccggugauua cugaaagcac ugaaaauagu aaaaugaugu uagaacuaga uccaccuuuu     1200

ggagauaguu auauaguuau aggaguagga gaaaagaaaa uaacacacca cuggcacaga     1260

ucaggaucaa caauaggaaa agcauuugaa gcaacaguaa gaggagcaaa aagaauggcu     1320

guacuaggag auacagcaug ggacuuuggu agu                                  1353


<210>  2710
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE CDS opt

<400>  2710
ucacaaaaag uaauauauuu aguaaugaua cuccucaucg caccagcaua cucaauaaga       60

uguauaggag uaucaaacag agacuuugua gaaggaaugu ccggaggaac uuggguugau      120

guuguauuag aacauggagg auguguaacc guaauggcac aagacaaacc aacaguagau      180

auugaacuag uaacaacaac aguaucaaau auggcagaag uuagaaguua uuguuacgaa      240

gcaucaauau cagauauggc aucagauuca aggugcccaa cacaaggaga agcauauuua      300

gacaaacaau cagacacaca auauguaugc aaaagaacac uggucgauag aggaugggga      360

aacggaugug gauuguucgg aaaaggauca uuaguuaccu gugcaaaauu cgcaugcagc      420

aaaaaaauga caggaaaaag uauacaacca gaaaauuuag aauacagaau aauguuaagu      480

guucacggau cacaacauag uggaaugauu guuaacgaca caggacacga aacagaugaa      540

aauagagcaa aaguagaaau aacgccaaau ucgccaagag cagaagcaac auuaggugga      600

uuuggaagcu uagguuuaga uugugaacca agaacaggac uugauuuuag cgauuuauac      660

uaccuaacaa ugaauaauaa acauugguua guucacaaag aaugguuuca ugauauacca      720

uuaccauggc acgcaggagc agauacagga acaccacacu ggaauaacaa agaagcacuu      780

gucgaauuua aagaugcgca cgcaaaaaga caaacaguag uuguucuagg aucacaagaa      840

ggagcaguac auacagcucu agcuggagca cuagaagcag aaauggacgg agcaaaaggc      900

agauuaucca guggacauuu aaaauguaga cuaaaaaugg acaaauuaag acuuaaagga      960

guuaguuauu cacuauguac agccgcauuc acauucacaa aaauuccagc agaaacacua     1020

cauggaacag uaacagucga aguacaauau gcaggaacag auggaccaug uaaaguaccg     1080

gcacaaaugg cuguagacac ucagacauua acaccaguag gaagguuaau uacagcaaau     1140

ccaguaauaa cugaaucaac agaaaauagc aaaaugaugu uagaauuaga uccaccauuu     1200

ggugauaguu auauuguuau aggaguagga gaaaaaaaaa uaacacauca uuggcaucga     1260

ucaggaagca caauaggaaa agcauuugaa gccacuguaa gaggagcaaa aagaauggca     1320

guacucggug auacagcgug ggacuuugga agc                                  1353


<210>  2711
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE CDS opt

<400>  2711
ucacaaaaag uaauauaccu aguaaugaua uuauuaauag cuccagcaua uaguauaaga       60

ugcauaggag uaaguaaucg agauuuugua gagggaauga gcggggguac auggguagac      120

guaguauugg aacacggagg auguguaaca guaauggcac aagacaaacc aacaguagac      180

auugaacugg uuacgaccac uguaucaaau auggcagaaa uaagaucaua cugcuaugaa      240

gcgaguauaa gugauauggc aucagauucu agauguccaa cacaaggaga agcauaucua      300

gacaaacaaa gugauacaca guauguaugu aaaagaacgc uaguagauag aggaugggga      360

aauggaugug gucuauuugg gaaagguagc uuaguaacau gugcaaaauu ugcaugcagu      420

aaaaaaauga caggaaaauc aauucaacca gaaaauuuag aauauagaau aauguuaucu      480

gugcauggau cacaacauuc aggaaugaua gugaacgaua caggacacga aacugaugaa      540

aauagagcaa aagucgaaau aaccccaaau ucacccagag ccgaagcaac auuaggagga      600

uuuggaucac uaggccuuga uugugaacca agaacaggau uagacuuuuc agacuuguau      660

uaucuaacaa ugaauaacaa acauugguua guacauaaag aaugguuuca ugauauacca      720

uuaccuuggc augcaggagc agauacgggu acaccacauu ggaauaauaa agaagcccuc      780

guagaauuua aagaugcaca ugcuaaaagg caaacuguug uaguacuagg uagucaagaa      840

ggagcuguac auacagcauu agcaggagca uuagaagcag aaauggacgg ggcaaaaggu      900

aggcucaguu caggacaccu aaaaugcaga uuaaaaaugg auaaauuaag acuaaaaggc      960

guaaguuaua guuuauguac agcagcauuu acauucacaa aaauaccagc agaaacguua     1020

cauggaacag uaacaguaga aguacaauau gcaggaacag auggaccuug caaaguucca     1080

gcacaaaugg caguagauau gcaaacacua acaccaguag gaagacuaau aaccgcaaau     1140

ccaguaauaa cagagagcac agaaaauagc aaaaugaugc uagaauuaga cccaccauuc     1200

ggagauuccu auauaguaau aggaguaggu gaaaaaaaaa uuacacauca cuggcaccga     1260

ucaggaucaa caauagguaa ggcuuucgaa gcaacaguaa gaggagcuaa acguauggca     1320

guauuaggag auacugcaug ggauuuugga uca                                  1353


<210>  2712
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE CDS opt

<400>  2712
ucacaaaaag uaauauacuu aguuaugauu uuguuaauag caccagcaua uucaauaaga       60

ugcauuggag uaucaaauag agauuucgua gaaggaauga gcggaggcac auggguagau      120

guaguauuag aacauggugg auguguuacu guaauggcac aagauaaacc aacuguagac      180

auagaacuag uaacaacaac aguaucaaau auggcagaag uaagaaguua uuguuaugaa      240

gcaagcauau ccgauauggc cucagacagu agauguccaa cacaaggaga agcuuacuua      300

gauaaacaau cagauacaca auauguaugu aaaagaacac uaguagacag aggaugggga      360

aauggaugcg guuuauucgg aaaaggaagu cuaguaacau gcgcaaaauu ugcauguagu      420

aaaaaaauga caggaaaaag uauacaacca gaaaauuuag aauacagaau aaugcuaagu      480

guccauggaa gucaacauag uggaaugauu guaaaugaca caggacauga aacagaugaa      540

aauagagcga aaguagaaau aacaccaaac ucaccaagag cagaagcaac auuaggagga      600

uuuggaucau uaggucuuga uugugaacca agaacuggac uugauuuuuc agauuuauau      660

uaccuaacaa ugaauaauaa acacuggcug guacauaaag aaugguucca ugacauaccu      720

cuacccuggc acgcaggagc agauacaggu accccacauu ggaauaauaa agaagcauua      780

guagaguuca aagaugcaca ugcaaaaaga caaacaguag uuguauuagg aucacaagaa      840

ggagcaguac auacagcacu agcaggagca uuagaagcag aaauggacgg cgcaaaaggc      900

agacuuagca guggacaccu aaaaugccgc uuaaaaaugg auaaacuaag auuaaaagga      960

guaaguuauu cauugugcac agcagcauuc acauucacaa aaauacccgc agaaacacua     1020

cacgguacag uuacaguuga aguacaauau gcaggaacag auggaccaug uaaaguucca     1080

gcacaaaugg caguagauau gcaaacauua acaccaguag gucgauuaau aacagcaaac     1140

ccaguaauua cagaaucaac agaaaauagu aaaaugaugu uagaacucga ccccccauuc     1200

ggagacucau acauaguuau aggaguaggu gaaaaaaaaa uuacacauca cuggcacaga     1260

ucaggaucaa caauaggaaa agcuuuugaa gcaacaguua gaggagcacg aagaauggcu     1320

guauuaggag auaccgcgug ggacuuugga agu                                  1353


<210>  2713
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE CDS opt

<400>  2713
ucucaaaaag uuauauauuu aguaaugauu uuauuaauag caccagcaua uucaauuaga       60

uguauaggag uaucaaauag agauuuugua gagggaaugu cuggaggaac auggguagau      120

guaguucuag aacauggagg auguguaacc guaauggcgc aagauaaacc aacugucgau      180

auagaauuag uaacaacaac cguuucaaau auggcagaag uaagaucaua uuguuaugaa      240

gcaucaauaa gugauauggu aagcgacuca agauguccaa cacaaggaga agcauaucug      300

gauaaacaau cggacacgca auauguaugu aaaaggacuc uaguugauag aggaugggga      360

aauggaugcg guuuauuugg uaaaggaagu uuaguaacau gcgcaaaauu ugcauguuca      420

aaaaaaauga caggaaaauc cauacaaccu gaaaauuuag aauacagaau aauguuauca      480

guacauggaa gucaacauuc aggaaugaua guaaacgaca caggacauga aacagaugaa      540

aacagagcua aaguggaaau aacaccgaau agcccaagag cagaggcaac auuaggagga      600

uuuggaucac ugggauuaga uugugaacca agaacaggcu uagauuuuag cgacuuauac      660

uauuuaacaa ugaauaauaa acauuggcua gugcauaaag aaugguucca cgauauacca      720

uuaccguggc augcaggagc agauacagga acaccacauu ggaauaacaa agaagcuuua      780

guagaauuua aagacgccca ugcaaaaaga caaacgguag uaguauuggg aucacaagaa      840

ggugcaguac auacagcacu agcaggagca uuagaagcag aaauggaugg cgcaaaagga      900

agacuaucgu caggacacuu aaaauguaga uuaaagaugg acaaacuuag auuaaaaggc      960

guaaguuauu cauugugcac agcagcauuu acauuuacaa aaauaccugc agaaacauua     1020

cauggaacag uaacuguaga aguacaauau gcaggaacag acgguccaug caaaguacca     1080

gcacaaaugg caguagauau gcaaacacua acaccaguag gaagacuaau aaccgcaaau     1140

ccagucauaa cagaaucaac agaaaauagc aaaaugaugc uagaauuaga uccuccauuc     1200

ggagauagcu auauagucau agggguuggg gaaaaaaaaa uuacucacca cuggcauaga     1260

aguggaagca ccauaggaaa agcauuugaa gcaacaguaa gaggagccaa aagaauggca     1320

guguuaggag auacggcaug ggauuuugga uca                                  1353


<210>  2714
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE CDS opt

<400>  2714
agucaaaaag uaauauaccu aguaaugaua cuauuaauag caccugcaua uaguauaaga       60

uguauaggag uaucaaauag agauuucgua gaaggaaugu caggaggcac auggguagac      120

guaguuuuag aacauggagg auguguaacu guaauggcac aagauaaacc uacuguagau      180

auagaauuag uaacaacaac aguaucaaac auggcagaag uaagaaguua uuguuaugaa      240

gcaagcauau cugauauggc aucagacuca agaugcccua cacaaggaga agcguacuua      300

gacaaacaaa gcgauacaca auauguaugu aaaagaacac ucguagauag aggauggggg      360

aacggaugug guuuauucgg gaaaggaucc cuaguaacau gugcaaaauu ugcauguagu      420

aaaaaaauga cagggaaauc aauacaaccu gaaaaccuag aauauagaau aauguuaagc      480

guacauggau cacaacauuc aggaaugauu guaaacgaca caggacauga aacagaugaa      540

aauagagcaa aaguagaaau uacaccaaau ucaccaaggg cugaagcaac auuaggaggu      600

uuuggaaguu uaggauuaga uugugaacca agaacaggac uagauuucag ugauuuguac      660

uacuuaacaa ugaauaauaa acauuggcuu guucauaaag aaugguucca ugauauacca      720

cuaccauggc acgcaggagc agauacugga acaccucacu ggaacaauaa agaagcacua      780

guugaauuca aagacgccca ugcaaaacga caaacugucg uaguauuagg aucacaagaa      840

ggagcaguac auacagcauu agcaggagca uuagaagccg aaauggaugg agcuaaagga      900

agauuaucau caggacaucu aaaguguagg cuaaaaaugg acaaacuaag auuaaaagga      960

gugaguuaca guuuauguac agccgcauuu acauuuacaa aaaucccagc agaaacacug     1020

cauggaacug uaacaguuga aguacaauau gcaggaacag augggccaug caaaguacca     1080

gcacaaaugg caguagauau gcaaacacua acaccaguag gaagguuaau aacagcuaau     1140

ccaguaauaa cagaaaguau agaaaauagu aaaaugaugu uagaacuaga cccaccuuuc     1200

ggugauucuu auauaguaau aggaguaggu gaaaaaaaaa uaacacauca uuggcauaga     1260

ucuggaagua ccauaggaaa agcauuugaa gcuacaguaa gaggagcuaa aagaauggca     1320

guauuaggag auacagcaug ggauuucggu agu                                  1353


<210>  2715
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE CDS opt

<400>  2715
ucacaaaaag uaauauaccu aguaaugaua uuacuaauag caccagcuua cucaauaaga       60

uguauagggg uaucaaauag agauuuuguc gaaggaaugu cuggaggaac auggguagau      120

gucguacuug aacacggagg auguguaaca guaauggcac aagauaaacc aacaguagau      180

auagaauuag uuacaacaac aguaucaaac auggcugaag uaagaucaua cuguuacgaa      240

gccucaauuu cagauauggc aagugauucu agguguccaa cgcaaggaga agccuauuua      300

gauaaacaau ccgauacaca auauguuugu aaaagaacuu uaguagauag aggauggggu      360

aacggaugug gauuauucgg aaaagggucu cuaguaacau gugcaaaauu ugcauguagu      420

aaaaaaauga caggaaaauc aauacaaccu gaaaaucuag aauacagaau aauguuaagu      480

guacauggaa gccaacauuc aggaaugaua guaaaugaua caggacauga aacagaugaa      540

aaccgugcca aaguagaaau aacaccaaac ucaccacgag cagaagcaac uuuaggagga      600

uuugggucac uaggacuaga cugugaacca agaacuggac uugacuuuuc ugaucuauau      660

uauuuaacca ugaauaacaa acacuggcuu guacacaaag aaugguucca ugauauccca      720

uuaccauggc acgcaggagc agauacagga acaccccauu ggaauaauaa agaagcauua      780

guagaauuua aagaugcaca cgcaaaacga caaacagucg uuguauuagg aucacaagaa      840

ggagcaguac auacagcacu agcaggagca cuagaagcag aaauggaugg agcaaaagga      900

agauuaucaa gcggacaccu aaaauguaga cuaaaaaugg auaaauuaag auuaaaagga      960

guaaguuaua guuuauguac agcagcauuc acauucacaa aaauaccagc agaaacacua     1020

cauggaacag ucacaguaga aguacaauau gcuggaacag auggaccuug uaaaguacca     1080

gcacaaaugg caguagauau gcaaacauua acuccaguag gaagauuaau uacagcaaau     1140

ccuguaauaa cagaaucaac cgaaaacuca aaaaugaugu uagaauuaga cccaccauuu     1200

ggagacucau auauagucau aggaauaggc gaaaaaaaaa uaacacauca cuggcacaga     1260

aguggcucaa caauaggaaa agcauuugaa gcgacaguaa ggggagcaaa aagaauggcc     1320

guauuaggag acaccgcaug ggacuuugga agc                                  1353


<210>  2716
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE CDS opt

<400>  2716
ucgcaaaaag uaauauaucu gguuaugauu uuguuaauag cauuagcaua cucaauaaga       60

ugcauaggag uaaguaauag ggauuuugua gaaggaauga guggagguac auggguagau      120

guaguauuag aacauggugg augcguaaca guaauggcac aagauaaacc uacaguagac      180

auagaauuag uaaccaccac aguuucaaac auggcagaag ucagaucaua cuguuacgaa      240

gcaagcauau ccgauauggc aucagacucu agauguccaa cccaagguga agcauauuua      300

gacaaacaau cagacacaca auauguaugu aaacgaacuu uaguagauag aggaugggga      360

aaugguugcg gauuauuugg aaaaggauca uuaguaacau gugcaaaauu cgcaugcagu      420

aaaaaaauga cuggaaaauc aauacaaccc gaaaauuuag aauauagaau aauguuaagu      480

guucauggca gucaacauag ugguaugaua guaaaugaca caggacauga aacagaugaa      540

aacagagcaa aaguugaaau aacaccaaac ucuccaagag cagaagcaac auuaggaggu      600

uuugguaguu uaggacuaga uugugaacca agaacuggau uagauuuuuc agaucuauau      660

uauuugacaa ugaauaauaa acauugguua guucacaaag aaugguucca ugauauacca      720

cuaccauggc augcaggagc agauacagga acaccacauu ggaauaauaa agaagcauua      780

guggaauuua aagaugcaca ugcaaaacga caaacaguag uuguacuagg aucacaagaa      840

ggagcaguac acacggcauu agcaggagca uuagaagcag aaauggaugg ggcaaaagga      900

agguuaagcu ccgggcauuu aaaaugccga uuaaaaaugg acaaauuaag auuaaaaggu      960

gugucuuaua gucuauguac agcagcauuu acauuuacaa aaauaccggc agaaacacuu     1020

caugguacag uaacaguaga aguacaauau gcaggaacag auggcccaug uaaagugcca     1080

gcacaaaugg caguagacau gcaaacacua acaccagucg gaagauuaau aacugcaaau     1140

ccaguaauaa cagaaucaac agaaaacagu aaaaugaugu uagaauuaga cccuccguuc     1200

ggagacuccu auauaguaau aggaguaggu gaaaaaaaaa uaacacauca uuggcauaga     1260

ucaggaucaa caauaggaaa agccuuugaa gcaacaguaa gaggagcaaa aagaauggca     1320

guacuaggag acacagcaug ggacuuugga ucc                                  1353


<210>  2717
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE CDS opt

<400>  2717
ucacaaaaag uaauuuacuu aguuaugaua cuacuuauag caccagcaua uucaauaaga       60

uguauaggag uaucaaauag ggacuuugua gaaggaauga guggugguac uuggguagac      120

guaguauuag aacacggagg auguguaacu guaauggcgc aagauaaacc aacaguagac      180

auagaauuag uuacaaccac cguaaguaau auggcagaag uaagaaguua uugcuacgaa      240

gcaaguauau cagacauggc aagugauuca agauguccaa cucaagguga agcauauuua      300

gauaaacaau cagauacaca auauguaugu aaaagaacuc uaguagacag aggauggggu      360

aauggaugcg gcuuauucgg aaaaggaucc uuaguuacau gugcuaaauu ugcuuguagu      420

aagaaaauga caggaaaaag uauucaacca gaaaacuuag aauacagaau aauguugagc      480

guacauggua gucagcauuc aggaaugaua gucaaugaua caggacauga aacagaugaa      540

aauagagcaa aaguagaaau aacaccaaau aguccaagag cagaagcgac auuaggagga      600

uuuggaagcc uaggacuaga cugugaacca agaaccgguu uagauuucuc cgauuuauac      660

uauuuaacaa ugaacaacaa acacuggcua guccauaaag aaugguuuca ugauauucca      720

cuaccauggc augcaggagc agacacgggu acaccacacu ggaauaauaa agaagcauua      780

guagaauuua aagaugcaca ugcaaagaga cagacaguag uuguauuagg aacacaagaa      840

ggugcaguac acacagcacu agcuggugca cuagaagcag aaauggaugg agcaaaaggc      900

cgacuaagua guggacaccu uaaauguaga uuaaaaaugg auaaauuaag auuaaaagga      960

guuucuuaua guuuauguac agcagcauuc acauuuacaa aaauaccagc ugaaacauua     1020

cacggaacag uaacaguuga aguucaauau gcaggaacag auggaccaug uaaaguaccu     1080

gcgcaaaugg cuguagauau gcaaacguua acaccaguag gaagauuaau aacugcaaac     1140

ccaguuauaa cagaaucuac agaaaauagu aaaaugaugu uagaauuaga cccuccauuu     1200

ggcgauaguu auauaguaau aggaguagga gaaaagaaaa uaacacacca cuggcauaga     1260

ucaggaagua caauaggaaa agcauuugaa gcuacaguac gaggcgcuaa aaggauggcu     1320

guauuaggug auacagcaug ggauuuugga uca                                  1353


<210>  2718
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE CDS opt

<400>  2718
agccaaaaag uuauauauuu aguuaugaua cuauuaauag caccagcuua caguauaaga       60

uguauaggag uaaguaauag agauuuugua gaggguaugu caggaggaac auggguagac      120

guaguauuag aacauggagg auguguaaca guaauggcac aagauaaacc aacaguagau      180

auagaacuag uaacgacaac aguaucaaau auggcugaag uuaggucaua uuguuaugaa      240

gcaaguauua gugacauggc aucagauagc agauguccaa cacaaggaga agcauaucua      300

gacaaacaaa gugauacaca auauguaugc aaaagaaccu uaguagauag aggaugggga      360

aacggcugug gucuauuugg aaaaggauca uuaguaacau gcgcaaaauu ugcaugcagu      420

aaaaaaauga caggaaaauc aauacaaccc gaaaaucuag aauauagaau aauguuaagu      480

guacauggau cacaacacuc gggcaugaua guaaaugaua caggacauga aacagaugaa      540

aauagagcga aaguagaaau aacaccaaau aguccuagag ccgaagcaac guuaggagga      600

uuuggcucac uuggacugga uugugaacca agaaccggac uagauuuuuc agauuuauau      660

uauuuaacaa ugaacaauaa acauugguua guacacaaag aaugguuuca ugauaucccc      720

uuaccauggc acgcaggugc agauacagga acaccacauu ggaauaauaa agaggcauua      780

guagaauuua aagacgcaca ugcaaaaaga caaacaguag uaguauuagg aagucaagaa      840

ggagcaguac auacagcauu agcuggagcc cuagaagcgg aaauggacgg agcaaaagga      900

agguuaucaa guggacauuu aaaauguaga cuaaaaaugg auaaauuaag acuaaaagga      960

guaucauaca gcuuguguac agcagcauuu acauuuacaa aaauaccagc agaaacauua     1020

cacgguacag uuacaguaga aguacaauau gcaggaacag acggaccuug caaaguauua     1080

gcacaaaugg ccguagauau gcaaacacua acaccaguag gaagauuaau aacagcaaau     1140

ccaguaauaa cagaaagcac agaaaacagu aaaaugaugu uagaacucga uccacccuuc     1200

ggagauuccu acauagucau agguguggga gaaaaaaaaa uuacacauca uuggcauaga     1260

agcggaagca caauaggcaa agcauuugaa gcaacaguca gaggagcaaa gcgaauggca     1320

guucuaggag acacagccug ggauuuugga agu                                  1353


<210>  2719
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE CDS opt

<400>  2719
agccaaaaag uaauauauuu aguaaugaua uuacuaauag caccagcaua uaguauacgc       60

uguauuggag uaucgaaucg agacuuugua gaaggaauga gcggaggaac auggguagau      120

guaguacuag aacacggagg auguguuacc guuauggcac aggauaaacc aacaguagau      180

auagaauuag uaacaacaac aguuaguaau auggcagaag uaagaagcua uuguuacgaa      240

gcaucaauaa gcgacauggc aagcgauagu agaugcccaa cacaaggaga agcauauuua      300

gauaaacaaa gugauacuca auauguaugc aaacgaacau uaguagacag aggaugggga      360

aauggaugug gauuauuugg aaaaggaagu cuaguuacau gcgcaaaauu ugcuuguagc      420

aagaaaauga caggaaaauc aauacaacca gaaaacuugg aauauagaau aauguuaucu      480

guacauggau cacaacauuc aggaaugaua guaaacgaua cuggacacga aacagaugaa      540

aauagagcaa aaguugaaau uacacccaau agcccaagag cagaagcaac auuaggggga      600

uucggaucau uaggauuaga cugugaacca agaacaggau uagacuucuc agauuuguau      660

uauuuaacaa ugaauaauaa acacuggcua guacacaaag aaugguuuca ugauauaccu      720

cucccauggc acgcaggagc ugauacagga acaccacacu ggaauaacaa agaagcauua      780

guagaauuua aagaagcaca ugcaaaaaga caaacuguug uaguacuagg aagucaagaa      840

ggagcaguac acacugcauu agcaggagcu uuagaagcgg aaauggaugg agcaaaagga      900

agauuaucaa guggacaucu aaaauguaga cuaaaaaugg auaaacuaag auuaaaaggc      960

guaaguuaua gucuauguac ggcugcguuc acauucacaa aaauaccagc agaaacauua     1020

cacggaacag uaacaguaga aguacaauau gcaggcacag auggacccug caaaguacca     1080

gcacaaaugg caguagauau gcaaacauua acaccaguag gaagacuaau aacagcaaau     1140

ccuguaauaa cagaaaguac cgaaaauagu aaaaugaugu uagaacuaga uccaccauuu     1200

ggagauaguu auauaguaau cggaguugga gaaaagaaaa uaacucauca cuggcacaga     1260

agcgguagua ccauagguaa agcauuugaa gcuacagugc gaggagcaaa aagaauggca     1320

guuuuaggag auacagcaug ggauuuugga agu                                  1353


<210>  2720
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE CDS opt

<400>  2720
ucacaaaaag uaauauacuu aguuaugaua uuacucaucg cuccggcaua uaguauaaga       60

uguauuggag uaucaaauag agauuuugua gaaggaaugu caggaggaac auggguagau      120

gugguauuag aacauggcgg augcguaaca guaauggcac aagauaaacc aacaguagac      180

auagaauuag ucaccacaac agucagcaau auggcagaag uaagaagcua uugcuaugaa      240

gcaucaauaa gugauauggc aagugauagc agauguccaa cacaaggaga agcuuaucua      300

gauaaacaaa gugauacaca auacguaugu aaaagaacau uaguugacag aggaugggga      360

aacggcugug gacuauuugg aaaaggaagu uuaguaacau gcgcaaaauu cgcauguagc      420

aaaaaaauga caggaaaaag uauucaacca gaaaaccuag aauauagaau aauguuaagc      480

guacauggaa gucaacauuc aggaaugaua guuaacgaua caggacauga aacagaugaa      540

aauagagcaa aaguagaaau aaccccaaau aguccuagag cagaagcagc auuaggagga      600

uuuggaucac uaggauuaga uugcgagcca agaacagggu uagauuuuuc agauuuauau      660

uaucuaacaa ugaauaacaa acauugguua guacacaaag aaugguucca cgauauacca      720

uuaccauggc acgcaggcgc ggacacagga acaccccauu ggaacaauaa agaagcauua      780

guagaauuua aagaugcgca ugcaaaacga caaacuguag uaguacuugg aagucaagaa      840

ggagcugugc auacagcucu ggcaggagcu uuagaagcag aaauggaugg agcaaaagga      900

agauuaucaa guggucacuu aaaauguaga uuaaaaaugg auaaacuaag guuaaaaggu      960

gugagcuaca guuuaugcac agcugcauuu acauuuacca aaauaccugc agaaacacua     1020

cacggcacag uuacuguaga aguccaauau gcaggaacag acggaccaug uaaagugcca     1080

gcacaaaugg caguagacau gcaaacauua acaccagugg ggagacuaau aacagcaaau     1140

ccaguaauaa cagaaaguac agaaaauagu aaaaugaugu uagaauuaga uccaccauuc     1200

ggagauucau auauagucau aggugugggu gaaaaaaaaa uuacacauca uuggcauaga     1260

ucaggaucaa cgaucgguaa agcauuugaa gcaacaguac guggggcgaa acgaauggca     1320

guauuaggag auacggcaug ggacuuugga uca                                  1353


<210>  2721
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE CDS opt

<400>  2721
ucacaaaaag uaauauaucu aguaaugaua uuauuaauag caccggcaua uagcauaaga       60

uguaucggag uaaguaauag agacuuugua gaaggaauga guggaggaac auggguugau      120

guaguacuag aacauggagg augcgugaca guaauggcuc aagauaaacc aacaguagau      180

auagaacuag uaagcacaac aguaaguaau auggcagaag uuagaagcua uuguuacgaa      240

gcaacaauaa gugauauugc aagugacagu agauguccaa cacaaggaga agcauaucua      300

gauaaacaaa gugauacaca auauguaugu aaaagaacgc uuguagauag aggaugggga      360

aauggcugug gacuauuugg aaaaggaagc cuaguaacau gugcaaaauu cgcaugcagu      420

aaaaaaauga caggaaaaag uauacagcca gaaaauuuag aauacagaau aaugcuaagu      480

guacauggau cacaacacag uggaaugaua guaaacgaua caggacauga aacagaugaa      540

aacagagcaa aaguagaaau aacaccgaau agcccgaggg cugaagcaac auuaggagga      600

uuuggaagcu uaggauuaga uugugaacca agaacaggau uagauuuuag ugacuuauac      660

uauuuaacaa ugaauaauaa acacugguua guacauaaag aaugguucca ugacauaccc      720

cuaccuuggc augcaggugc agauacagga acaccacauu ggaauaauaa agaagcauua      780

guagaauuua aagaugcuca ugcuaaaaga caaacagcag uuguacuagg aucacaagaa      840

ggagcaguac auacagcacu cgcaggggca uuagaagcag aaauggacgg agcaaaaggc      900

agauuaucaa gcgggcauuu aaaauguaga cuaaaaaugg auaaauuaag acuaaaaggu      960

gugucauauu cacuauguac agcugcauuc acauuuacaa aaauaccagc agaaacauua     1020

cacggaacgg ucacagucga aguccaauac gcaggaacag auggaccaug uaaaguaccc     1080

gcacagaugg caguagauau gcaaacacuu acaccaguag guagauuaau aacagcaaau     1140

ccaguaauaa cagaaaguac agaaaacagu aaaaugaugc uagaauuaga uccaccauuu     1200

ggagauucau auauaguaau uggagugggc gaaaaaaaaa uuacacauca cuggcaucga     1260

aguggaucaa caauaggaaa agcauuugaa gcaaccguaa ggggagcuaa aaggauggca     1320

gucuuaggag acacagcuug ggauuucggu uca                                  1353


<210>  2722
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE CDS opt

<400>  2722
agucaaaaag uaauauaucu agugaugaua uuauuaauag caccagcaua uagcauaaga       60

uguauaggag ugucaaacag agauuucgua gaaggaaugu caggaggaac auggguagau      120

gugguacuag aacauggagg uugcguuacc guaauggcac aagauaaacc aacaguagac      180

auagaauuag uaacaaccac cguaaguaau auggcagaag uuagaaguua uuguuaugaa      240

gcaagcauaa gcgacauggc aagcgacagc agauguccaa cacaaggaga agcauauuua      300

gauaaacaaa gcgacacaca auauguaugc aaaagaacuu uaguagauag ggguugggga      360

aauggcugug gacuauuugg aaaagguagu uuaguaacau gugcaaaauu ugcauguuca      420

aagaaaauga cuggaaaauc aauacaacca gaaaauuuag aauaucguau aaugcuaagu      480

guacauggau cacaacacag uggaaugaua guaaaugaua caggacauga aacagaugaa      540

aauagagcaa aaguagaaau aacaccaaac ucacccagag cugaagcaac guuaggagga      600

uuuggaucau uaggauuaga uugugaacca aggacaggau uagauuuuag ugaucuauau      660

uaucuaacaa ugaauaauaa acacugguua guucauaaag aaugguuuca ugauauacca      720

uuaccauggc augcaggagc agacacagga acaccacauu ggaacaauaa agaagcgcua      780

guggaauuua aagaugcaca cgcaaaaaga caaacuguag uuguacuagg aagucaagaa      840

ggggcaguac auacggcucu agcuggagca cuggaagcag aaauggacgg ggcuaaaggu      900

agauuaucga gcggacauuu aaaauguagg uugaaaaugg acaaacuuag auuaaaaggu      960

guaucauaua guuuauguac agcagcguuu acguuuagua aaauaccugc agaaacauua     1020

cacggaacag uaacagaaga aguacaauac gcaggaacgg auggucccug caaaguucca     1080

gcacaaaugg cuguggacau gcaaacauua acaccuguag gaagauuaau aacagcaaac     1140

ccaguaauaa cagaaagcac agaaaauagu aaaaugaugu uagaauuaga uccaccauuu     1200

ggagauaguu auaucguaau aggaguagga gaaaagaaaa ucacucauca cuggcauaga     1260

agcgguagua caauaggaaa agcauuugaa gcaacaguaa gaggagcaaa aagaauggca     1320

guacuaggag auacagcgug ggauuuugga uca                                  1353


<210>  2723
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE CDS opt

<400>  2723
ucacaaaaag uaauuuauuu aguuaugaua uuacuaauag caccagcaua uucaauaaga       60

uguauaggag ugaguaaucg agauuuugua gaaggcauga guggaggaac uuggguagau      120

guaguuuuag aacacggcgg augcguaaca guaauggcac aagauaaacc gacgguagac      180

auagaauuag uaacaacaac aguuaguaac auggcagaag uaagaucaua uuguuaugaa      240

gcaaguauaa gugauauggc uagugauuca agauguccaa cacaaggcga agcauacuua      300

gauaaacaaa gcgauacaca auauguaugu aagagaacau uaguagauag agguugggga      360

aauggaugug gauuauuugg aaaaggauca cuaguaaccu gcgcaaaauu ugcuuguuca      420

aaaaaaauga caggaaaauc aauccaacca gaaaaucuag aauauagaau aauguuauca      480

guacauggga gccaacacuc gggaaugaua guaaaugaua cuggccacga aacagacgaa      540

aaucgagcaa aaguagaaau aacaccaaau ucaccaagag cagaagcaac acuaggagga      600

uuuggaucau uaggauuaga cugugaacca agaacuggac uagauuuuuc agaccuauac      660

uaucuaacaa ugaauaauaa acacugguua guacauaaag aaugguuuca ugacauacca      720

cugccauggc augcaggcgc agauacggga acgccacauu ggaacaauaa agaagcauua      780

guugaauuua aagaugcaca ugcgaaaaga caaacuguag uaguauuagg aagccaagaa      840

ggagcaguuc acaccgcauu agcuggagcg uuagaagcag aaauggacgg agcaaaaggg      900

agauuaucaa gcggucaucu aaaaugcaga cuaaaaaugg auaaauuaag auuaaaagga      960

guaagcuauu cgcuauguac agcagcauuu acauuuacaa aaauaccagc agaaacauua     1020

cauggaacag uaacaguaga auuacaauau gcuggcacag auggaccaug uaaaguacca     1080

gcacaaaugg caguagauau gcaaacucuu acaccaguag gacgauuaau uacagcaaau     1140

ccaguaauaa cugaaucuac ugaaaacagu aaaaugaugu uagaauuaga uccaccauuu     1200

ggagauaguu auauaguaau aggaguagga gaaaaaaaaa uaacacauca uuggcauaga     1260

ucagguagua caauaggaaa agcauuugaa gcaacaguaa gaggagcaaa aagaauggca     1320

guuuuaggug auacagccug ggauuuuggu ucg                                  1353


<210>  2724
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE CDS opt

<400>  2724
agucaaaaag ugaucuauuu aguaaugauu cuacuaauag caccagcaua uucaauaaga       60

uguauaggca uaucuaacag ggauuuuguu gaaggaauga guggaggaac uuggguagac      120

guaguauugg aacauggagg auguguaaca guaauggcac aagauaaacc uacaguagac      180

auagaacugg uaacaacaac aguaaguaau auggcugaag uaaggucaua cuguuacgaa      240

gcaaguauaa gcgauauggc aagugauuca agaugcccaa cacaaggaga agcauaucuc      300

gauaaacaaa gcgauacaca auauguaugu aaaagaacau uaguagauag aggaugggga      360

aacgguugcg gauuauuugg gaaaggaucu uuaguaacau gugcaaaauu cgcauguagu      420

aaaaaaauga caggaaaauc cauacaacca gaaaacuuag aauauagaau aauguuaagu      480

guacacggua gucaacauuc aggaaugauu guaaaugaua caggacacga aaccgacgaa      540

aauagagcaa aaguagaaau aacaccaaac ucaccaagag cagaagcaac acuaggagga      600

uuuggaauac uuggauuaga uugugaacca cgaacuggac uagauuuuuc agaccuauau      660

uaucuaacga ugaauaauaa acauuggcua guacauaaag aaugguuuca ugauauaccc      720

uuaccauggc augcaggcgc agauacggga acgccacauu ggaacaacaa agaagcauua      780

guagaauuua aagaugcaca ugcaaaacga caaacaguag uaguauuagg gucacaagaa      840

ggagcaguac auacugcacu ugcaggagcu uuagaagcag aaauggacgg ugcaaaagga      900

agauuaucau caggacacuu aaaauguaga uuaaaaaugg acaaauuacg auuaaaagga      960

guaucauaua gcuuaugcac cgcagcauuu acauuuacua aaauaccugc ugaaacauua     1020

cauggaaccg uaacaguaga aguacaauau gcaggaacag auggaccaug uaaaguacca     1080

gcacaaaugg caguagauau gcaaacacua acaccaguag gaagacuuau cacagcaaau     1140

ccaguaauaa cagaaagcac ggagaauuca aaaaugaugu uagaacuaga uccaccauuu     1200

ggagacucau acauaguaau aggaguagga gaaaaaaaaa uaacacauca uuggcacaga     1260

ucaggaagua cuauaggaaa agcauuugaa gcaacgguaa gaggagcaaa gagaauggca     1320

guauuaggag auacagcaug ggauuucgga uca                                  1353


<210>  2725
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE CDS opt

<400>  2725
ucacaaaaag uaauauauuu aguaaugaua uuauuaauag caccagcaua uaguauaaga       60

uguauaggag uaaguaauag agauuucgua gaaggaauga gugggggaac auggguagau      120

guaguauuag aacauggagg guguguaaca guaauggcac aagacaaacc aaccguggau      180

auugaauuag uaacuacuac agucaguaau auggcagaag ucagaucaua uuguuacgaa      240

gcuaguauau cagacauggc gagugauagc agauguccaa cacaaggaga agcauauuua      300

gauaaacaaa gugauacuca auauguaugu aaaagaacau uaguugauag agggugggga      360

aauggaugug gauuauuugg aaaaggaagc uuaguaacau gugcaaaauu cgcauguagu      420

aaaaaaauga caggaaaaag uauacaacca gaaaacuuag aauauagaau aaugcugagu      480

guacauggau cacaacauuc aggaaugaua guaaaugaua caggacauga gacagaugaa      540

aacagagcaa agguagaaau aacuccaaac aguccacgag cagaagcaac acuaggugga      600

uuuggaucac uaggacuaga uugugaaccu agaacaggau uagauuuuuc agauuuauau      660

uaucuuacaa ugaacaacaa acauuggcua guacauaaag aaugguucca cgauauacca      720

cuaccauggc augcuggagc cgauaccggc acuccacauu ggaacaauaa agaagcauua      780

guagaauuca aagaugcaca cgcuaaaaga cagacagugg uaguauuagg aagucaagaa      840

ggagcaguac acacagcacu agcaggagcc uuagaagcag aaauggaugg gguaaaaggu      900

agguuaagca guggacacuu aaaauguaga uuaaaaaugg auaaacuaag acuuaaaggu      960

guaaguuacu cauuauguac agcagcuuuu acguuuacua aaaucccugc agaaacacua     1020

cauggaacag uaaccgugga aguacaauac gcaggaacag auggaccaug uaaaguacca     1080

gcacaaaugg cuguagauau gcaaacauua accccaguag gaagauugau aacagcaaau     1140

ccaguaauaa cagaaaguac agaaaauagu aaaaugaugu uagaauuaga uccucccuuu     1200

ggugauagcu auauaguuau agguguagga gaaaaaaaaa uaacacauca uuggcacaga     1260

ucaggcagca caauagguaa agcuuuugaa gcaacaguaa gaggagcaaa acgaauggca     1320

guguugggag auacagccug ggauuuugga uca                                  1353


<210>  2726
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE CDS opt

<400>  2726
agccaaaaag uaauuuauuu aguaaugaua cuauuaauag caccagcaua uaguauaaga       60

uguauagggg uauccaacag agauuucguu gaaggaauga gcggaggaac auggguagau      120

guaguuuuag aacauggugg auguguaaca guaauggcac aagauaaacc uacaguagau      180

auagaauuag uaacaacaac aguaucaaac auggcagaag uaagaaguua uugcuaugaa      240

gcaagcauua gugauauggc aagugauagu cgauguccaa cacaaggaga agcauaucua      300

gacaaacaaa gugacacaca auacguaugu aaaagaacau uaguagauag aggauggggu      360

aauggcugcg gacuauucgg gaaaggaucc uuaguaacuu gugcaaaauu ugccuguagu      420

aaaaaaauga caggaaaauc aauacaaccc gaaaaucuag aauaccgaau aaugcuaagu      480

guacacggaa gucaacauuc aggaaugauc gugaaugaca cagggcauga aacugaugaa      540

aacagagcaa aaguagaaau uacaccaaau ucaccaaggg cugaagcaac auuaggagga      600

uucggaaguu uaggacugga uugugaaccc agaacuggau uagacuuuuc agauuuauac      660

uauuuaacaa ugaauaacaa acacuggcua guacauaaag aaugguuuca cgauauacca      720

uuaccauggc augcaggagc agauacagga acaccacauu ggaauaauaa agaagcacua      780

guagaauuua aagaugcaca cgcuaaaaga caaaccguug uaguauuagg aucacaagaa      840

ggagcgguuc acacagcacu agccggagca uuagaagcgg aaauggaugg agcaaaagga      900

agauuaucau caggucacuu aaaauguagg uuaaaaaugg auaaacuaag acuaaaagga      960

guaagcuaua gucuuuguac agcagcauuc acauucacaa aaauaccagc agaaacauua     1020

cauggaacag uaacaguuga aguacaauau gcaggagcag acggaccaug caaaguaccu     1080

gcacaaaugg caguggauau gcaaacauua acuccaguag guagguuaau aacagcaaac     1140

ccaguaauaa cagaaaguac ggaaaauuca aaaaugaugc uagaauuaga uccaccauuu     1200

ggagauaguu auauaguaau aggaguagga gaaaaaaaaa uuacacacca uuggcauaga     1260

ucagguagua caauaggaaa agcauuugaa gcaaccguac guggagcuaa aagaauggcu     1320

guauuaggag auacagcaug ggacuuuggu agu                                  1353


<210>  2727
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE CDS opt

<400>  2727
ucacaaaaag uaauauaucu aguuaugaua cuauuaauag caccagccua caguauacga       60

uguauuggag uaaguaauag agauuuuguu gaaggaauga gugggggaac auggguagau      120

gugguacuug aacauggagg augugucaca guaauggcac aagauaaacc aacaguggau      180

auagaauuag uaacaacuac aguaaguaac auggcagaag uaagaaguua uugcuacgaa      240

gcuucaauaa gugacauggc aagugauagc agauguccga cacaaggcga agcauaucua      300

gauaaacaaa gcgauacuca auauguaugu aaaagaacau uaguagacag agguuggggc      360

aauggaugug gauuauuugg uaaaggcucu cuaguaacau gugcaaaauu ugcauguuca      420

aaaaaaauga caggaaaauc aauucaacca gaaaaucuag aauauagaau aauguuauca      480

guccauggaa gucaacauuc agguaugaua guaaacgaca caggacauga aacagaugaa      540

aacagagcaa aaguagaaau cacaccaaau aguccaagag ccgaagcaac uuuaggagga      600

uucggaucau uaggauuaga uugcgaacca agaaccgggu uagauuuuag ugauuuauau      660

uaucuuacua ugaauaauaa acauuggcua guacauaaag aaugguuuca ugauauacca      720

uuaccuuggc acacuggagc agauacagga acaccacauu ggaacaauaa agaagcuuua      780

guagaauuua aagaugcaca ugcaaaaaga caaacaguag uaguauuagg aagucaagaa      840

ggagcuguac auacugcauu agcuggugca uuagaagcag aaauggaugg agcaaaagga      900

aggcuaagca guggacaucu aaaauguaga uuaaaaaugg auaaacuaag auuaaaagga      960

guaucauaua gucuauguac agcagcauuc acauuuacaa agauaccagc agaaacacua     1020

cacgguacag uaacgguaga aguccaauau gcaggaacag auggcccuug uaaagugcca     1080

gcacaaaugg caguagauau gcaaacacua acuccaguag gaagacuuau aacagcaaau     1140

ccaguuauaa cagaaggcac agaaaacagu aaaaugaugu uagaacucga uccaccauuu     1200

ggcgauucau auauaguaau aggaguagga gaaaaaaaaa uaacacauca uuggcauagg     1260

ucuggaucaa cuauaggaaa agcauuugag gcaacuguaa gaggagcaaa aagaauggca     1320

guuuuaggug auacagcaug ggauuuugga uca                                  1353


<210>  2728
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE CDS opt

<400>  2728
ucacaaaaag uaauauauuu aguaaugaua uuguuaauag caccagcgua caguauuaga       60

uguauaggag uuagcaauag agauuuugua gaaggaauga guggaggaac cuggguagau      120

guaguauuag aacauggggg auguguaaca gcaauggcgc aagauaaacc aacaguagac      180

auagaauuag uaacaacuac cguuaguaau auggcagaag uacgaagcua uuguuacgaa      240

gcuucaauaa gugauauggc aagugacagc agauguccaa cacaaggaga agcguaccua      300

gauaaacaaa gugauacaca auacguaugc aaaaggacuu uaguagauag aggaugggga      360

aauggaugug guuuauuugg gaaaggaagc cuuguaacau gugcaaaauu ugcauguagc      420

aaaaaaauga cagguaaauc aauacaaccu gaaaaucuag aauauagaau aauguuaagc      480

guacauggau cacaacauag cggaauguua guaaacgaua caggacauga aacagaugaa      540

aauagagcaa aaguagaaau aacgccaaac ucaccucgag ccgaagcaac auuaggugga      600

uuuggaucau uaggauuaga uugcgaacca agaacaggcu uagacuuuag cgauuuauau      660

uacuuaacga ugaacaauaa acauugguua gcucauaaag aaugguuuca ugauauacca      720

cuaccauggc acgcgggagc agcaacagga acgccucauu ggaauaauaa agaagcauua      780

guagaauuua aagaugcaca ugcuaaaaga caaacaguag uaguacuagg aagccaagaa      840

ggagcaguac acacagcauu ggcaggagca uuggaagcag aaauggaugg agcaaaagga      900

cgauuaaguu cuggacauuu aaaaugucga uuaaaaaugg auaaacuaag acucaaaggg      960

guaucauacu cauuauguac agcagcguuu acauucacaa aaauaccagc agaaacagua     1020

gaugguacag uaacaguaga aggacaauau gguggaacag acggaccaug caaagugcca     1080

gcacaaaugg caguagauau gcaaacacuu acaccaguag gaagauuaau aacagcaaau     1140

ccagugauaa cagaaucgac agaaaacagu aaaaugaugu uagaacuaga uccaccuuuu     1200

ggagauucau auauuguaau aggaguaggg gaaaaaaaaa uaacacauca uuggcauaga     1260

ucuggaagca cgauaggaaa agcuuuugaa gcaacaguaa gaggagcaaa aagaauggca     1320

guuuuagggg auacagcaug ggauuucgga agc                                  1353


<210>  2729
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE CDS opt

<400>  2729
agccaaaaag uuauauaccu aguaaugaua uuauuaauag caccagccua uaguauacga       60

ugcauuggag uaucaaauag agauuucgua gaaggcaugu cagguggaac auggguagau      120

guaguauuag aacauggagg auguguaaca guaauggcac aagacaaacc agcaguagau      180

auagagcuag uaacaacuac aguaucaaau auggcagaag uaagaaguua cuguuaugaa      240

gcuagcauaa gcgauauggc aucugauucu agguguccaa cacaaggcga agcauauuua      300

gauaaacaau cugauacaca auacgucugu aaaagaacuc uaguagauag aggaugggga      360

aacggaugug guuuauucgg aaaaggcagc uuaguuacau gugcaaaauu ugcaugcuca      420

aagaaaauga caggaaaauc aauacaaccu gaaaaccuag aauacagaau aauguuauca      480

gugcacggau cacaacauag cggaaugaua gucaaugaua caggacauga aacagaugaa      540

aacagagcua aagucgaaau aacaccaaac aguccaagag cugaagcaac auuaggagga      600

uucgguagcu uaggacuuga uugcgaacca agaacaggac uagauuuuuc agaucuuuau      660

uacuuaacca ugaacaacaa acauugguug guacacaaag aaugguuuca ugauauacca      720

cuaccuuggc augcaggagc agauacugga acaccacauu ggaacaauaa agaagcauua      780

guagaauuua aagacgcuca ugcaaaaaga caaacagucg uuguauuagg aucacaagaa      840

ggagcaguac auaccgcucu agcuggagca uuagaagcag aaauggaugg agcaaaagga      900

agauuaucau caggacacuu aaaaugcaga cuaaaaaugg acaaauuaag acucaaagga      960

guaucuuauu cauuaugcac cgcagcauuu acauuuacaa aaauaccagc agaaacacua     1020

cauggaacug uaacagucga aguccaauau gcaggaacag auggaccgug caaagucccg     1080

gcacaaaugg caguagacau gcaaacuuua acaccaguag gacgauuaau aacugcaaau     1140

ccuguaauaa cagaaagcac agaaaauuca aaaaugaugc uagaauuaga uccacccuuc     1200

ggagauucau auaucguaau aggaguagga gaaaagaaaa uaacacauca uuggcauaga     1260

ucaggaagca cuauagguaa agcauuugaa gcaacaguaa gaggugcaaa aagaauggcc     1320

guuuuaggug auacagcaug ggauuuuggc agu                                  1353


<210>  2730
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE CDS opt

<400>  2730
agucaaaaag uaauauauuu agugaugaua cuauuaauag caccagcaua uaguauaaga       60

uguauaggug ugaauaauag agauuuuguu gaaggaaugu cagggggaac auggguagau      120

gucgucuuag aacacggagg uuguguaaca gugauggcac aagauaaacc aacaguagau      180

auagaauuag uaacaaccac aguaaguaau auggcagaag uaagaucaua uuguuaugag      240

gcaaguauaa gugauauggc aagugauagu agauguccga cacagggaga agcauauuua      300

gauaaacaaa gcgauacuca auauguaugu aaaagaacau uaguagauag aggaugggga      360

aauggaugcg gauuguuugg aaaaggaagu cuagugacau gugcaaaauu ugcaugcucc      420

aaaaaaauga caggaaaauc aauacaacca gagaacuuag aauauagaau aauguuaagu      480

guacauggaa gucaacacag cggaaugaua guuaaugaua caggacauga aacagaugaa      540

aaucgagcga aaguugaaau aacuccaaac ucaccuagag cagaagcaac auuaggaggg      600

uucggaucau uaggacuaga uugugaacca agaacaggac uagauuucag ugacuuauau      660

uaucuaacaa ugaauaauaa acauuggcua guacauaaag aaugguuuca ugauauaccc      720

uuaccauggc augcgggugc agauacaggu acaccacauu ggaauaauaa agaagcccuu      780

guggaauuua aagaugcaca cgcaaaaaga caaacaguag ucguacuagg uucacaagaa      840

ggagccgugc acacagccuu agcaggagca cuggaagcag aaauggaugg ggcaaaagga      900

agauuaucga guggacaucu aaaaugcaga cuaaaaaugg auaaauuaag auuaaaagga      960

guaaguuauu cauuauguac agcagcauuu acauuuacaa aaauaccagc agaaacauua     1020

cauggaacag uaacaguuga aguacaauau gcgggaacag acggaccaug uaaaguacca     1080

gcacaaaugg caguagacau gcaaaccuua accccagucg gaagacuaau aacagcaaac     1140

ccgguaauaa ccgaaaguac agaaaacucu aaaaugaugu uagaacuaga uccaccauuu     1200

ggagauaguu acauaguaau aggaguggga gaaaagaaaa uaacacauca cuggcauaga     1260

agcggaagca caauuggcaa agcauucgaa gcaaccguaa gaggagcaaa aagaauggca     1320

guauuaggag auacugcaug ggauuuugga uca                                  1353


<210>  2731
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE CDS opt

<400>  2731
ucacaaaaag uaauauauuu agucaugaua uugcuaauag caccugcaua uaguauaaga       60

uguauaggag uaaguaauag agacuuugua gaaggaauga gcggcggaac auggguagac      120

guaguacuag aacauggagg auguguaaca guaauggcac aagauaaacc aacaguagau      180

auagaauuag uaacaacaac aguaucuaac auggcugaag uaagaaguua uugcuacgaa      240

gcaaguauaa gcgauauggc aucagauucc agauguccga cacaaggaga agcauauuua      300

gauaaacaaa gcgauacaca auaugugugc aaaagaacau uaguagauag aggaugggga      360

aauggaugcg gacuauuugg aaaagguagc uuaguaacau gugcaaaauu ugcauguucu      420

aaaaaaauga caggaaaaag cauacaacca gaaaauuuag aauaucgaau aauguuaagc      480

guacauggau cacagcauuc aggaaugaua guaaacgaua caggucauga aacagacgaa      540

aauagagcaa aaguagaaau aacaccuaac uccccaagag cagaagcaac auuaggagga      600

uuuggaucac uaggauuaga uugugaacca agaacuggac uugacuucag ugacuuauau      660

uauuuaacaa ugaauaauaa acauugguua guacauaaag aaugguuuca cgacauacca      720

uuaccauggc augcaggagc agauacagga acuccccauu ggaauaauaa agaagcauua      780

guagaauuua aagaugcaca cgcaaaaaga caaacaguag uaguacuugg aagucaagaa      840

ggagcaguac auacagcuuu agcaggagca cuggaagcag aaauggacgg ugcuaaagga      900

agacuuucaa guggacaucu aaaaugucgg uuaaaaaugg auaaauuaag auuaaaaggc      960

guaaguuaua gcuuaugugc agcagcauuu acuuuuacaa aaauaccugc agaaacacua     1020

cacgguacag uuacgguaga aguccaauau gcuggaacgg auggaccaug uaaaguaccu     1080

gcacaaaugg caguagauau gcagacauua acuccagugg gaagauuaau cacagcaaau     1140

ccaguaauaa cagaaaguac agaaaauagu aaaaugaugc uagaauuaga uccaccauuc     1200

ggggauaguu auauaguaau aggaguaggu gaaaaaaaaa uaacacauca cuggcauaga     1260

uccggaucga caauaggaaa agcauuugaa gcaacaguaa gaggggcuaa aagaauggca     1320

guauuaggag auacagcuug ggauuuugga uca                                  1353


<210>  2732
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE CDS opt

<400>  2732
ucacaaaaag uaauauaccu aguaaugaua uuauuaauag caccagcaua uagcauaaga       60

uguauaggag uaaguaauag agacuuuguc gaaggaauga guggaggaac auggguagac      120

guaguauuag aacauggagg auguguaaca guuauggcac aagauaaacc aacaguagac      180

auagaacuag uaacaacuac aguuucaaau auggcugaag uaagaaguua uuguuacgaa      240

gcaaguauua gcgauauggc gagugauuca agaugcccaa cucaaggaga agcauaucua      300

gauaaacaaa gugauacaca auauguaugu aaaagaacac uaguagacag aggaugggga      360

aauggaugug gauuauuugg aaaaggauca uuaguuacau gugcaaaauu ugcauguuca      420

aaaaaaauga caggaaaaag uauacaaccu gaaaauuugg aauauagaau aaugcugagu      480

guacauggau cacaacacag uggaaugaua gucaaugaua uuggacauga aacagaugaa      540

aaucgagcaa aaguagaaau aacaccgaau agcccaagag cagaagcaac uuuaggagga      600

uuuggaucau uagggcuaga uugugaaccu agaacuggac uagauuuuag ugacuuguau      660

uacuuaacaa ugaauaacaa acacuggcug guacauaaag aaugguuuca ugauauacca      720

uuaccauggc acgcaggugc agacacagga acaccacauu ggaauaauaa agaagcauua      780

guagaauuca aagaugcaca ugcaaaaaga caaacagugg uaguauuagg aagucaagaa      840

ggagcaguac auacugcauu agcaggugcu uuagaagcag aaauggaugg agcaaaagga      900

agauuaucuu caggacauuu aaaaugcaga uuaaaaaugg acaaauuaag guuaaaagga      960

guaaguuaca gccuuuguac agcagcauuu acuuuuacga aaauccccgc agaaacuuua     1020

cauggaacag uaacaguaga aguacaauau gcaggaacag acggaccaug caaaguacca     1080

gcacaaaugg cagucgauau gcaaacauua acaccuguag gaagacucau aacagcgaau     1140

ccgguuauua cugaaucaac agaaaauagu aaaaugaugc uagaauuaga cccaccauuu     1200

ggagauucgu auauaguaau aggaguaggc gauaaaaaaa uaacacauca uuggcauaga     1260

ucaggaucaa caauaggaaa agcauuugaa gcaacaguaa gaggagcaaa aagaauggca     1320

guauuaggag auacagcaug ggacuuugga ucg                                  1353


<210>  2733
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE CDS opt

<400>  2733
ucgcaaaaag uaauauauuu aguaaugauu cuauuaauag cuccugcaua uucaauaaga       60

uguauaggug uaagcaauag agauuuugua gaaggaauga guggaggaac guggguagau      120

guaguauuag aacacggagg auguguaacu guaauggcac aagauaaacc aacuguagau      180

auagaauuag uaacaacuac aguaucaaau auggccgaag uaagaagcua uugcuaugaa      240

gcuaguauaa gugacauggc aucagacuca agauguccaa cacaaggcga agcauaccua      300

gauaaacaaa gugauacaca auaugucugc aaaaggacgc uaguagauag aggaugggga      360

aauggaugug gucuauuugg aaaaggguca uuagucacau gugcaaaauu ugcauguuca      420

aagaaaauga caggaaaauc aauacagcca gaaaauuuag aauauagaau uauguuaagu      480

guacacggaa gccaacacuc aggaaugauu guaaacgaca caggacauga aaccgaugaa      540

aacagagcaa aaguagaaau aacaccuaau ucaccaagag cagaagcaac guuaggagga      600

uuugguucuu uaggacuaga uugugaacca aggacaggac uagauuuuag ugacuuauau      660

uacuuaacaa ugaacaauaa acauuggcua guucauaaag aaugguuuca ugauauaccu      720

cuaccauggc acgccggagc agauacugga accccacauu ggaauaauaa agaagcauua      780

guagaauuua aagaugcaca cgcaaaaaga caaacuguag uaguauuagg uucucaagaa      840

ggugcaguac acacagcuuu agcaggagca uuagaagcag aaauggaugg ugcaaaagga      900

agacuaagua guggacacuu aaaauguaga uuaaaaaugg auaaacuaag auuaaaagga      960

guaucauauu cauuauguac ugcagcauuc acauucacaa aaauuccagc agaaacuuua     1020

cauggaacag uuacaguuga aguacaauau gcaggaacag auggaccaug caaaguacca     1080

gcgcaaaugg caguagauau gcaaacauua acuccaguag gacgauuaau aaccgcaaau     1140

ccaguaauaa cagaaaguac agaaaacagu aaaaugaugc uugaauuaga cccaccauuu     1200

ggagauucau auauuguaau agguguagga gacaagaaaa uaacacacca cugguacaga     1260

ucgggaucaa cgauaggaaa agcauuugaa gcaacaguaa gaggagcaaa aagaauggca     1320

guauuaggag acacagcaug ggauuuugga uca                                  1353


<210>  2734
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE CDS opt

<400>  2734
ucacaaaaag ugauauauuu aguaaugaua uuacuaauag caccagcaua caguauaaga       60

uguauaggag uaucuaauag agauuuugua gaaggaaugu caggaggaac auggguagau      120

guaguacuag aacacggagg auguguaaca guaauggcac aagacaaacc aacaguagau      180

auagaauuag uaacaacaac uguaagcaac auggcagaag uuagaaguua uuguuaugaa      240

gcaaguauaa gugauauggc aagugauuca agauguccua cacaaggaga agcauacuua      300

gacaaacaaa gcgauacaca auauguuugc aaaagaacgu uagucgaccg aggauggggg      360

aacggaugug gacuauuugg aaaaggaagu cuaguaacau gugcuaaauu uacauguagc      420

aaaaaaauga caggaaaauc aauacaacca gaaaauuuag aauacagaau aauguuaagu      480

gugcauggau cacaacauag cggaaugaua guuaaugaua cugggcacga aacugaugaa      540

aauagagcga aaguagaagu cacaccaaac aguccaagag cggaagcaac uuuaggagga      600

uucgguucau uaggacuaga uugcgaaccu aggacuggau uagauuuuuc agaucuauau      660

uaucuuacaa ugaauaauaa acauugguua guacauaaag aaugguuuca ugauauacca      720

cuaccguggc augcaggagc agauacagga acaccucauu ggaauaacaa agaagcauua      780

guagaauuca aagaugcaca ugcaaaacga caaacgguag uaguacuugg aagucaagaa      840

ggagcaguac acacagcauu agcaggagcu uuagaagccg agauggacgg ggcaaaaggc      900

agacuauuua gcggacacuu aaaaugcaga uuaaaaaugg auaaauuaag auuaaaggga      960

guaaguuauu cauuauguac agcugcauuu acuuuuacaa aaguuccagc agagacauua     1020

cauggaacag uaacagugga aguacaauau gcaggaacag augggccaug uaaaguucca     1080

gcacaaaugg caguagauau gcaaacauua acuccaguag gaagacuuau uacagcaaau     1140

ccaguaauaa cagaaaguac agaaaauagu aaaaugaugc uugaauuaga cccaccauuu     1200

ggggauucuu auauagucau aggaguugga gauaaaaaaa uaacucauca uuggcauaga     1260

ucuggcucaa cuauaggaaa agcguuugaa gcaacgguaa gaggagcaaa aagaauggcu     1320

guacuaggcg acacagcuug ggauuuugga uca                                  1353


<210>  2735
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE CDS opt

<400>  2735
ucacaaaaag uuauauauuu aguaaugaua uuguuaauag caccggcaua uaguauaaga       60

uguauaggag uaucaaauag agauuuuguc gaaggaauga guggaggaac auggguugau      120

guaguauuag aacauggcgg augcguaacu guaauggcac aagauaaacc aacuguggau      180

auagaauuag uaacaacaac aguaagcaau auggcagaag uaagaaguua uuguuaugaa      240

gcaucaauaa gugauauggc aagugauucu cgaugcccga cacaaggaga agcauacuua      300

gauaaacaaa gugauacaca auauguaugc aagcgaacac uuguagacag aggaugggga      360

aacggaugug guuuauucgg uaaaggaagu uuaguaacuu gugcaaaauu uacauguagc      420

aaaaaaauga caggaaaauc aauacaaccu gagaauuuag aauauagaau uaugcuaagc      480

guacacgggu cacaacauag cggaaugaua guaaaugaua uaggacauga aacagaugaa      540

aauagagcaa aaguagaagu aacuccaaau aguccaagag cagaagcaac auuagggggc      600

uucgguucau uagguuuaga cugcgaacca cggacuggac uagacuucuc agauuuauau      660

uauuuaacaa ugaauaacaa acacuggcug guacauaaag aaugguuuca cgauauaccu      720

uuaccauggc augcgggcgc agauacagga acaccucauu ggaauaacaa agaagcacua      780

guagaauuua aagaugcaca ugcaaaacga caaacaguag uaguauuagg aagucaagaa      840

ggggcaguac auacagcacu agcgggagca uuagaggcag aaauggaugg agcaaaagga      900

agguuauuca gcggacauuu aaaauguaga cuuaaaaugg auaaauuaag auuaaaagga      960

guaagcuaua guuuauguac agcagcauuu acauuuacaa aaguaccagc agaaacauua     1020

cauggcacag uaaccguaga aguacaauau gcaggaacag auggaccaug caaaguacca     1080

gcucaaaugg cuguagauau gcaaacacua acaccuguag gaagacugau uacagcuaau     1140

ccagucauaa cagaaaguac ggaaaauagc aaaaugaugu uagaacuaga uccaccauuu     1200

ggggauagcu acauaguaau aggaguagga gauaaaaaaa uaacacauca cuggcauaga     1260

ucaggaucaa caauaggaaa agcauuugaa gcaacaguaa gaggagcaaa acgcauggca     1320

guauuagggg acacagcuug ggauuuugga uca                                  1353


<210>  2736
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE CDS opt

<400>  2736
agucaaaaag uuauauauuu aguuaugaua uuacuaauag caccagcaua uucaauaaga       60

uguauaggag uaagcaacag agauuuuguu gaaggaauga gcgguggaac auggguagac      120

guaguauuag aacauggagg auguguaaca guaauggcac aagauaaacc aacaguagac      180

auagaacuag uaacaacaac aguuucaaau auggcagaag uaagaaguua uugcuaugaa      240

gcaaguauaa gugacauggc aagugauagu agauguccaa cucaagguga agcauacuua      300

gauaaacaga gugacacaca auacguaugu aaacgaacuc uaguagacag aggcugggga      360

aauggaugug ggcuuuucgg aaaaggauca uuaguuacgu gugcaaaauu uacaugcagu      420

aaaaaaauga caggaaaauc aauacaacca gaaaaucugg aauauagaau aauguuaagc      480

guacauggaa gucaacauuc aggaaugaua guaaacgaua ucggacauga aacagaugaa      540

aacagagcaa aaguagaagu aacuccaaau agcccaagag cagaagcaac auuaggagga      600

uuuggaucau uaggucuaga uugugaacca agaacaggac uagauuuuag ugaucuauac      660

uaucuaacaa ugaauaauaa acauugguua guacacaaag aaugguucca ugauauacca      720

uuacccuggc augcaggagc agacacagga acaccacauu ggaacaauaa agaagcauua      780

guagaauuua aagacgcaca ugcaaaaaga caaacuguag uagugcuagg aagucaagaa      840

ggagcuguac acacagcacu agcaggugca uuagaggcag agauggaugg agcaaaagga      900

agauuauuua guggacaucu aaaguguaga cuaaaaaugg acaaauuaag auuaaaagga      960

guaaguuaua gucugugcac agcuguaugc acagcagcaa aaguaccagc agaaacuuua     1020

cauggaacag uaacuguaga aguacaauau gcagggacag augguccuug uaaaguacca     1080

gcucaaaugg caguagacau gcaaacgcua acaccaguag gaagacuaau cacagcaaau     1140

ccaguuauaa ccgaaaguac agaaaauagc aaaaugaugu uagaacucga uccuccauuu     1200

ggagauagcu auaucguaau aggaguagga gacaaaaaaa uaacucauca uuggcauaga     1260

aguggaucaa caauaggaaa agcauuugaa gcaacaguaa gaggugcaaa aagaauggca     1320

guacuaggag acacagcaug ggacuuugga uca                                  1353


<210>  2737
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE CDS opt

<400>  2737
ucacaaaaag uaauauacuu aguuaugaua uuacuaauag caccggcuua uaguauaaga       60

ugcauaggag uaagcaauag agauuuugua gaaggaauga gcggaggaac auggguagau      120

guaguauuag aacacggagg cugcguaaca guaauggcuc aagauaaacc aacuguagau      180

auagaacuag uaacaacuac aguaucaaau auggcagagg uaagaaguua uuguuaugaa      240

gcaucaauaa gcgauauggc aucagacagu agauguccaa cacaagguga agcauauuua      300

gauaaacaaa gugauacaca auauguaugu aaaagaaccc uaguagacag gggaugggga      360

aauggcugug gauuauuugg aaaaggauca cuaguuacau gugcaaaauu cacauguagc      420

aaaaaaauga caggaaaauc aauacaacca gaaaacuuag aauacagaau aauguuaagu      480

guacacggaa gccaacauuc agguaugaua guaaaugaug aaaacagagc aaaaguagaa      540

guuacaccaa auucaccaag agcagaagca acacuaggag gauuugguuc ucuuggauua      600

gauugugagc caagaacagg ucuggauuuu agugacuuau auuacuuaac aaugaauaau      660

aaacauuggu uaguucacaa agaaugguuc caugauauuc ccuuaccaug gcauucagga      720

gcagauacag aaacaccaca uuggaauaau aaagaagcau uaguagaauu uaaagaugca      780

caugcaaaaa gacaaacagu uguuguacug ggaagccaag aaggggcagu acauacagca      840

uuagcuggag cacuagaagc cgaaauggau ggagcgaaag gcagacuauc aaguggacau      900

uuaaaaugua gauuaaaaau ggauaaauua agauuaaaag gaguaagcua cucauuaugu      960

acagcagcau ucacauuuac aaaaguacca gcagaaacau uacaugguac aguaacagua     1020

gaaguccaau augcuggaag agauggacca uguaaaguac cagcacaaau ggcaguagau     1080

augcaaacau uaacaccagu ugguagauua auaacagcua auccagugau aacugaauca     1140

acagaaaaua guaagaugau guuagaacua gauccaccau ucggagacag uuauauagua     1200

auaggagucg gagauaaaaa aauaacgcau cauuggcaua gaagcggaag uauuauagga     1260

aaagcauucg aagcaacagu aagaggagca aaaagaaugg cgguacuagg agauacagcu     1320

ugggauuuug guagu                                                      1335


<210>  2738
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE CDS opt

<400>  2738
ucgcaaaaag uaauauaucu cguuaugaua guucuaauag uaccagcuua caguauaagc       60

uguauaggag uaucaaauag agacuuagug gaaggcauga gcggagguac auggguagac      120

guaguauuag aacacggagg augcguaaca gaaauggccc aagauaaacc aacuguggau      180

auagaauuag uaacgaugac aguaagcaau auggcugagg uaagaagcua uuguuacgaa      240

gcaaguuuau cagauauggc uagcgcauca agguguccaa cacaaggcga accaucauua      300

gauaaacaaa gugauacuca aaguguaugu aaaagaacuc uaggagauag gggaugggga      360

aauggaugcg gaauauucgg uaaaggaagu cuaguaacau gcaguaaauu cacauguugu      420

aaaaaaaugc cuggaaaauc aauucaacca gaaaauuuag aauacagaau aaugcuacca      480

guacauggaa gucaacauag cggaaugaua guuaaugaua uaggacauga aacugaugaa      540

aacagagcaa aaguagaagu aacuccaaac agcccuagag cagaagcaac acucggagga      600

uucggaucac ucggauuaga uugugaacca agaacaggac uugacuucag ugaucuuuac      660

uauuuaacga ugaauaacaa acauugguua guacauaaag aaugguucca ugauauacca      720

uuaccauggc augcaggagc agauacagga acaccacauu ggaacaauaa agaagcguua      780

guagaauuua aagaugcaca ugcaaaaaga caaacaguag uaguauuagg aagccaagaa      840

ggagcuguac acacggcacu agcaggggca uuagaagcag aaauggaugg agccaaaggu      900

agauuauuua guggacauuu aaaauguaga uuaaaaaugg auaaacuaag acuaaaagga      960

guaaguuaca gcuuauguac agcagcauuu acauucacaa agguaccagc cgaaaccuua     1020

cacggaacag uuacaguaga aguccaaagu gcaggcacag augguccaug uaaaguaccg     1080

gcacaaaugg caguagauau gcaaacuuua acaccuguag gaagguuaau aacagcaaau     1140

ccaguaauaa cagaaagcac agaaaauagu aaaaugaugc uagaauuaga cccaccauuu     1200

ggagauucau auauaguaau aggagucgga gauaaaaaaa uaacacauca uuggcacaga     1260

aguggaucaa caauaggaaa agcauuugaa gcaacaguaa gaggcgccaa aagaauggca     1320

guauuaggag auacagcaug ggauuuugga ucu                                  1353


<210>  2739
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE CDS opt

<400>  2739
agacaaaaag uaauauauuu aguaaugaua uuacuaauug caccugcaua uucaauaaga       60

ugcauaggug uuaguaauag agauuuuguu gaaggaaugu caggaggaac uuggguagac      120

guaguacuag aacauggugg cuguguaaca guaauggcac aagauaaacc aacaguagau      180

auagagcuag uuacgacaac aguuagcaac auggcagagg uaagaaguua uuguuaugaa      240

gcaaguauau cagacauggc aagcgauuca agguguccaa cacaagguga agcauacuua      300

gauaaacaaa gugauacaca auacguaugu aaaagaaccu uaguugauag agguugggga      360

aaugguugug gacuauuugg aaaaggaagu uuaguuacau gcgcaaaauu ugcauguagu      420

aaaaaaauga caggaaaauc aauacaacca gagaauuuag aauauagaau aaugcuuagu      480

guacauggaa gucaacauag cggaaugaua guaaaugaca caggacauga aacugaugaa      540

aauagagcaa aaguagaaau aacaccaaau ucacccagag cagaagcuac auuaggagga      600

uuuggaucau uaggauuaga cugugaaccu agaaccggau uagauuuuuc agaucuauau      660

uacuuaacaa ugaauaauaa acauugguua guacauaaag aaugguuuca ugauauaccg      720

uuaccauggc augcaggcgc agauacagga accccacacu ggaauaauaa agaagcacua      780

guagaauuua aagaugccca ugcaaaacgu caaacuguag uaguacuagg aucacaagaa      840

ggagcaguac acacugcauu agccggagcu uuagaagcag aaauggaugg agcgaaagga      900

agacuaucau cuggacaccu aaaauguaga uuaaaaaugg auaaauuaag auuaaaagga      960

guaaguuaua guuuaugcac agcagcauuc acauuuacga aaauuccagc agaaacauua     1020

cacggaacag uaacaguaga aguucaauau gcgggaacag acggaccaug uaaaguacca     1080

gcacaaaugg caguugauau gcagacauug acucccguug gaagacuaau aacagcaaau     1140

ccaguaauaa cugaaucaac agaaaacuca aaaaugaugc uagaauuaga uccaccauuu     1200

ggagauucau auauaguaau aggaguaggu gaaaaaaaaa uaacucauca uuggcacaga     1260

agugggagua caauaggaaa agcauuugaa gcaacuguaa gaggggcaaa acgaauggca     1320

guacuaggag auacugcaug ggauuuugga agc                                  1353


<210>  2740
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE CDS opt

<400>  2740
agucaaagag uaauauacuu aguaaugaua uuauugauag caccagcaua uucaauuaga       60

uguauaggag uaaguaauag agauuuugua gaaggaauga guggaggaac auggguagau      120

guaguauuag aacauggagg auguguaacu guuauggcac aagauaaacc aacaguagau      180

auagaauuag uaacaacaac aguaucaaau auggcagaag uaagaagcua cuguuacgaa      240

gcaucaaucu cugauauggc aagugauagu agauguccga cacaaggaga agcuuauuua      300

gauaaacaaa gcgauacaca auauguaugc aaaagaacau uaguugauag aggaugggga      360

aauggaugug gauuauuugg aaaagguagu cuaguaacau gugcuaaauu uacauguagc      420

aaaaaaauga caggaaaaag uauacaacca gaaaaucuag aauauaggau aaugcuauca      480

guacacggaa gucaacacag cggaaugauu guaaaugaca uaggacacga aacagaugaa      540

aaucgagcaa aaguagaagu aacuccaaau ucaccuagag cagaagcaac cuuaggaggu      600

uuugggucau uaggacuaga cugugaacca agaacaggau uagauuuuag ugacuuauau      660

uacuuaacaa ugaauaauaa acauuggcua guacacaaag aaugguuuca cgauauacca      720

cuaccauggc acgcaggagc agauacugga acaccacauu ggaauaauaa agaagcauug      780

guugaauuua aagaugcaca ugcuaaaaga caaaccguag uaguguuagg cagccaagaa      840

ggagcaguac auacagcuuu agcaggagca uuagaagcag aaauggaugg agcaaaaggu      900

agauuauuuu caggacaucu gaaauguaga auaaaaaugg auaaauuaag auuaaaagga      960

guauccuaua guuuauguac agcagcauuc acguuuagua aaguaccagc agaaacguua     1020

caugguacag uaacaguaga aguacaauau gcagguaggg acggaccgug uaaaguacca     1080

gcacaaaugg ccguagauau gcaaacauua acaccaguag gacgauuaau aacagcaaac     1140

ccaauaauaa cagaaucaac ugaaaauagu agaaugaugu uagaauuaga uccaccauuu     1200

ggagauucau auauaguuau agguguagga gauaaaaaaa uaacccauca uuggcauaga     1260

aguggcucaa caauagguaa ggcauuugaa gcgacaguaa gaggagcgaa aagaauggcu     1320

gugcuaggag auacugcaug ggauuuugga agc                                  1353


<210>  2741
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  2741
cguauaauca acgcacgaaa agagaagaaa aggcgcggcg ccgacacuuc agucgguauc       60

gucggccucc uccucacaac cgccauggcc gcagaaguca cccgccgagg aagugcauac      120

uacauguacc uagaccgcaa ugacgccgga gaagcaauca gcuuccccac cacacucggu      180

augaacaaau gcuacauaca aauaauggac cucggucaca ugugcgacgc cacgaugagc      240

uacgaaugcc ccaugcucga cgaagggguc gaaccagacg acguugacug cuggugcaac      300

acaaccucua cuugggucgu cuacgguacc ugccaccaca agaaaggcga agcgcgacga      360

ucccgcaggg ccgucacccu gccgagccau ucaacccgaa aacuacaaac ccgaagccaa      420

accuggcucg aaucucgcga auacaccaaa cacuugaucc gcgucgaaaa cuggauuuuc      480

agaaacccag gcuucgcacu cgcggcagcc gcaauagccu ggcuccucgg cucaucaaca      540

ucccagaaag ucaucuaccu cguaaugauu cuacucaucg ccccagcaua cucaauacgg      600

ugcauaggag uguccaaccg cgacuucguc gagggaaugu caggcggcac auggguagac      660

auaguacucg aacacggagg augcgucacc guaauggccc aagacaaacc aaccguugac      720

auagaacucg uaaccacaac cguaagcaac auggcagaag uccgcucaua cugcuacgag      780

gcauccauca gcgacauggc cuccgacagc cgaugcccca cccaaggaga ggccuaccuc      840

gauaaacaaa gcgacaccca auacgucugc aaaagaaccc ucguagauag agguuggggc      900

aacggcugcg gucuauucgg caaaggaagc cuaguaacau gcgccaaauu cgccugcucc      960

aagaaaauga ccggcaaauc cauccaaccg gagaaccuug aauauagaau caugcugucc     1020

guacacggau cccaacacuc cggaaugaua gucaacgaca caggccacga gaccgacgag     1080

aaccgagcaa aaguagagau cacacccaac ucgccaagag ccgaagccac ccuagguggc     1140

uucggaucac uuggccucga cugcgaaccc cggacagggc ucgacuucuc agaccucuac     1200

uaccucacca ugaacaacaa acacuggcuu guacacaaag aaugguuuca cgacauccca     1260

cucccauggc augcaggcgc agacaccggc acaccacacu ggaacaacaa agaagcccua     1320

gucgaguuca aagacgcaca cgcaaaacgc caaacagugg uaguccuggg uucccaagaa     1380

ggcgccgucc acacagcccu cgccggcgcc cucgaagcag aaauggacgg cgcaaaagga     1440

cgccuuagcu ccggccaccu aaaaugcaga cucaaaaugg acaaacuccg ccuuaaaggc     1500

guaucauaca gccucugcac agcugcauuc accuucacca aaauacccgc cgaaacccuc     1560

cacggaacag ucaccgucga aguacaauac gcaggaacgg auggcccuug caaaguacca     1620

gcccagaugg ccgucgacau gcagacccua acaccagucg gaagacucau aaccgccaau     1680

ccagucauaa cagaguccac cgaaaacucc aaaaugaugc ucgaacuaga cccuccauuu     1740

ggcgacuccu acauagucau aggcguagga gaaaagaaaa uaacacacca cuggcaccgc     1800

uccgguucca ccauuggaaa agcauucgaa gcgacaguac gcggggcaaa acguauggcg     1860

gugcucggcg acaccgccug ggacuuuggg uccgucggcg gcgcccucaa uucacucggc     1920

aaaggaauac aucaaaucuu cggagcggcc uucaaauccc uauucggcgg caugagcugg     1980

uucucccaaa uacugaucgg cacacuacuc auguggcuag gccucaacac caaaaacggc     2040

ucaaucuccc ucaugugccu ggcacucggc ggcguacuca ucuuccuauc cacagcagug     2100

agugccgacg ugggaugcuc cguagacuuc ucgaaa                               2136


<210>  2742
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) CDS opt

<400>  2742
cgcaucauca acgcccgaaa agaaaagaaa cgacgaggag cggacacguc agucggcauc       60

guaggauuac ugcucaccac agcaauggcc gccgaaguca cccgcagggg cagcgcauau      120

uacauguacc uugaccgaaa cgacgcgggg gaagcaauau cguuccccac cacgcuaggc      180

augaacaaau gcuacaucca aauaauggac cuaggccaua ccugcgacgc cacaauguca      240

uacgaaugcc caaugcuaga cgaaggcgua gaacccgacg acguagacug cuggugcaac      300

acaacaagca caugggucgu cuacgggaca ugccaccaca agaaaggcga agcucgcaga      360

agcagacgcg ccgucacacu gccaagccac uccacccgaa aacuacaaac ccgcagccaa      420

accuggcucg aaagccgcga auacacaaaa caccucauac gagucgaaaa cuggaucuuc      480

cgaaaccccg gcuucgcccu ggcagcagca gccauagccu ggcuccuagg cucaagcaca      540

agccaaaaag uaauauaccu agucaugauc cuacuaaucg cgccagcgua cucaauccga      600

ugcaucggug ucagcaaccg agacuucguc gaaggcaugu cagguggcac cugggucgau      660

gucguccugg aacacggcgg uugcgucacg gucauggccc aggauaaacc uaccguagac      720

auagaacugg ucaccacgac cguaagcaac auggcagaag ugaggagcua uugcuacgaa      780

gcaucaauau cggacauggc cucugauucg cgaugcccga cccaaggcga ggcauacuug      840

gauaaacaau cagauaccca auacguaugu aaacgaacgc uaguagaccg aggguggggg      900

aacgguugcg gacucuuugg aaaaggcucc cuaguaaccu gcgccaaauu cgcguguuca      960

aagaaaauga caggcaaauc aauacagcca gaaaaccucg aauaccggau aaugcucucc     1020

guccacgggu cccaacacag cggcaugaua gucaacgaca caggccacga aacagacgaa     1080

aauagggcca aagucgaaau aacaccaaac ucaccacgag cggaagccac gcucggcgga     1140

uucggauccc uaggccucga cugugaaccc cggaccggcc uggacuuuag cgaccuauac     1200

uaccucacaa ugaacaacaa acacuggcuc guccacaaag aaugguuuca cgacaucccc     1260

cugccauggc acgcgggcgc agacacuggc acaccacacu ggaacaacaa agaagcucuu     1320

gucgaguuca aagacgcaca cgccaaaaga caaacagucg ugguacucgg aucccaagaa     1380

ggagccguac acaccgcccu cgccggcgcc cucgaagccg agauggacgg ugccaaagga     1440

cgccucagca gcggacaccu aaaaugcaga cuaaaaaugg acaagcucag acucaagggc     1500

guauccuacu cccucugcac cgccgccuuc accuucacaa aaauccccgc cgaaacccua     1560

cacgggaccg ucaccgucga aguacaguac gccggcacag auggccccug uaaaguacca     1620

gcccaaaugg ccgucgacau gcaaacccug acacccgucg gacgccuaau aacggccaac     1680

cccgucauca ccgaaucaac cgaaaacucc aaaaugaugc ucgaacuaga cccacccuuc     1740

ggcgacagcu acaucgucau cggggucgga gaaaagaaaa uaacccacca cuggcaccga     1800

uccgggucaa ccaucggcaa agcauucgaa gccaccgucc gcggcgcaaa acgcauggcg     1860

guccugggag acacagcuug ggacuucgga uccgucggcg gcgcccuaaa cucacucgga     1920

aaaggcauuc accaaaucuu cggcgccgcc uuuaaaagcc ucuucggcgg aaugucgugg     1980

uucucccaga uccuuauagg aacccuacua auguggcucg gcuugaacgc caaaaacgga     2040

agcauaaguc ugaugugccu cgcacucgga ggcguccuaa ucuuccuaag caccgcagua     2100

agcgccgacg uuggauguag uguagacuuu uccaaa                               2136


<210>  2743
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) CDS opt

<400>  2743
agaauaauca acgcccgaaa agaacgcaaa cgcagaggag ccgacacaag cauaggcauu       60

aucggccuac uucucaccac cgcaauggcu gcagaaauca ccagacgagg aagcgccuau      120

uacauguauc ucgacagauc agaugcagga aaggcaaucu ccuucgccac cacacucgga      180

guaaacaaau gccacgugca aaucauggac cucggacaca ugugcgacgc gacaauguca      240

uacgagugcc ccaugcuaga cgagggaguc gagccagacg acguagacug cuggugcaac      300

acaaccucaa ccugggucgu cuacggaaca ugccaccaca aaaaggggga agcacgccga      360

agccgcagag cggucacacu accaagucac uccacacgca agcuccaaac ccggagccag      420

accuggcuag aaucccggga auacaccaaa caccuaauaa aaguagagaa cuggauauuu      480

cggaacccag gauucgcgcu cgucgccgua gccaucgcau ggcuacuagg cuccagcaca      540

ucacaaaaag ucauauaccu uguaaugauc cuccucaucg cccccgcaua cuccauccgc      600

ugcauaggag ucagcaaucg cgacuucguc gaaggcaugu caggaggaac cuggguagau      660

guaguacucg agcauggcgg cugcgucaca gucauggcac aagacaaacc aaccguagac      720

aucgaacucg ucacaaccac cgugagcaac auggcugaag uccggucaua cugcuacgag      780

gcaucaauau ccgacauggc cuccgacagc cgcugcccaa cccaaggcga agccuaccuc      840

gacaaacaga gcgacacaca auacgucugc aaacgaaccc ucguagacag aggcuggggg      900

aacggaugcg gacuauucgg caaaggaucc cucguaaccu gcgccaaguu cacgugcagc      960

aagaaaauga ccggcaaauc cauucaaccc gaaaaccuag aguaccgcau caugcuauca     1020

guccacggca gucaacacuc agggaugauc ggcuacgaaa ccgacgaaga ccgcgccaaa     1080

guggaaguca caccaaacuc accacgcgcc gaagcaacac uaggcggguu cggaucccug     1140

ggacucgacu gcgagccccg cacgggccua gacuuuagcg accuuuacua ucuaaccaug     1200

aacaacaaac acuggcuagu acacaaagag ugguuccacg acaucccacu accguggcac     1260

gcaggggcag auaccggcac accgcacugg aacaauaaag aagcccuagu agaguucaaa     1320

gacgcacacg ccaaacgaca aaccguagug gucuuaggau cccaggaagg agccguacac     1380

accgcccucg ccggagcccu agaagccgaa auggacggcg ccaaaggccg ccuauuuucg     1440

ggccaccuca aaugccgccu gaaaauggac aaacuacgcc uaaaaggggu aaguuacucc     1500

cucugcaccg ccgcauucac auucacuaaa gucccagccg aaacccucca cggcacagua     1560

acaguagaag uccaauacgc cggaaccgac ggacccugca aaauccccgu acaaauggca     1620

guagauaugc aaacccuaac ccccgucggc cgccuaauaa cagccaaucc cguaauuacc     1680

gaauccacag agaacagcaa gaugaugcuc gaacuugacc cacccuucgg cgacucguac     1740

auagucaucg gagucggcga caagaaaauu acacaccacu ggcaccgguc aggaucgaca     1800

aucggcaaag cguucgaggc aaccguacga ggagccaaaa gaauggccgu acuaggugac     1860

accgcguggg acuucggcuc cguagggggc guauucaacu cccuaggaaa aggaauccac     1920

cagaucuucg gcgccgcauu caagucacua uucgggggua ugagcugguu cagccaaauc     1980

cuaaucggca cccuacuggu guggcucggu cucaacacca aaaacggauc aaucucccuc     2040

acaugccuag cauuaggggg agucaugauc uuccucagca cagcagucuc agccgacgug     2100

ggaugcagcg uagacuucuc aaaa                                            2124


<210>  2744
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) CDS opt

<400>  2744
agaaucauca acgccaggaa agaaaagaaa agacgaggag cagauaccag uguaggcaua       60

guaggcuuac uccucacuac agcaauggca gccgaaguaa caagacgggg cuccgcguac      120

uacauguacc ucgaccgaaa cgacgcagga gaggcgauca gcuuccccac gacgcuaggg      180

augaacaaau gcuacauaca aaucauggac cucggccaca ugugcgacgc aacaaugagc      240

uacgaaugcc ccaugcugga ugaaggagug gagcccgacg acguagacug cuggugcaau      300

accacaagua ccuggguggu guacggaaca ugccaccaca agaaaggcga agcccgacga      360

ucgcgccgag ccguaacucu gccgucccau ucgaccagaa aacuccaaac aagaucacag      420

acauggcuag agucacggga auauacaaaa caccucaucc gcguagaaaa cuggauauuc      480

cgaaacccgg gcuucgcccu cgcagcggca gcaaucgccu ggcugcuggg gaguucgacc      540

agccaaaaag ucauauaccu aguaaugauc cuacucaucg caccggcuua cagcauacgc      600

ugcauaggag ucucaaaccg agacuucguc gaaggaauga gcggcggcac cuggguagac      660

guaguacucg agcacggcgg gugcguaacc gucauggcac aagacaaacc aacaguagac      720

auugagcuag ucacgacaac aguauccaac auggcugaag uaagaaguua cuguuacgaa      780

gccagcauca gcgacauggc aagcgacucu cgcugcccca cccaaggaga agccuaccuc      840

gacaaacagu cagacaccca guacguaugu aaacgaacgc uaguagacag aggcuggggc      900

aacggaugug gccuuuuugg aaaaggaucc uuagucacau gcgccaaauu cgccugcagc      960

aagaaaauga cagggaaauc aauccagcca gaaaaccuag aauaccggau caugcucagc     1020

guccacggcu cccagcacuc gggaaugauc guaaacgaca caggccacga aacagacgaa     1080

aaucgcgcca aaguagaaau aaccccgaac agcccccgcg cagaagccac ucuaggaggu     1140

uucggaagcc ucggucuaga cugcgagccc cgcaccgggc uagacuucag ugaccuauac     1200

uaccugacaa ugaacaacaa acacuggcua guccacaaag aaugguucca ugacauccca     1260

cuaccguggc acgcaggcgc agauaccggc accccacacu ggaauaacaa agaagcccuc     1320

gucgaguuca aagacgcgca cgccaaacgc caaacugugg ucguacucgg gagucaggaa     1380

ggcgcaguac acaccgcacu cgcaggcgcc cuagaagcag aaauggacgg cgcaaaagga     1440

cgccuaucca gcggacaccu caaguguaga cuaaaaaugg acaaacuccg acucaaaggu     1500

guaagcuacu cccuaugcac ggcagcauuc accuucacga aaauaccagc ggaaacacuc     1560

cacggaacag uaacaguaga aguccaauac gcagggacug acgggccaug caaaguaccc     1620

gcgcaaaugg caguagacau gcaaacacuc acaccggucg gacgacuaau aacagcaaac     1680

ccgguaauca ccgaaagcac cgaaaacagc aaaaugaugc ucgaacuaga cccacccuuc     1740

ggagacucau acaucgucau cggcguagga gaaaagaaaa ucacgcacca cuggcacaga     1800

ucaggaucga cuauaggcaa agccuucgaa gcaacaguca gaggggccaa acgcauggca     1860

guacuggggg acacagccug ggacuucggg uccgucggcg gggcucuaaa cagccuaggc     1920

aaagggauac accaaaucuu cggcgcagca uucaaaucac ucuucggcgg caugagcugg     1980

uucagccaaa uccuaaucgg aacacuacuc auguggcuag gguuaaacac caaaaacggc     2040

agcaucagcc ucaugugcuu agcccuaggc gggguacuca uauuucuaag cacagcagua     2100

agcgccgacg uaggaugcag cguagacuuc ucaaaa                               2136


<210>  2745
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  2745
acguccguag gcaucguggg gcuccugcua acgacggcca uggcagcaga gguaacccgc       60

cgaggcucag cauacuacau guaccuagac cgaaacgacg cgggagaagc caucagcuuc      120

cccaccaccc ucggaaugaa caaaugcuac auccaaauaa uggaccucgg ccacaugugc      180

gacgcgacaa ugucauacga augcccaaug uuagacgaag gggucgaacc cgacgacguc      240

gacugcuggu gcaacaccac aagcacaugg guaguauacg guaccugcca ccacaagaaa      300

ggcgaagcca gacgcagccg cagagccgua acccucccgu cccacagcac acggaaacuc      360

cagacacgga gucaaacaug gcuggaaucg cgcgaauaca ccaaacaccu uauaagagua      420

gaaaacugga ucuuccgaaa cccgggcuuc gcgcucgccg cagccgccau cgcauggcuc      480

cuggggucca gcaccagcca aaaagucaua uaccucguca ugauccuccu cauagccccc      540

gccuacucca uccgcugcau cggugugucc aaccgagacu ucguagaagg aaugucggga      600

ggaacauggg uagacauagu ccucgaacac ggcgggugcg ucaccgucau ggcgcaagac      660

aaaccaacag uagacauaga gcuaguaaca acaacaguca gcaacauggc ugaaguccga      720

uccuacugcu acgaagcauc aaucagcgac auggcaucug acagucgaug ccccacacaa      780

ggugaagccu accucgacaa acagucagac acacaauacg uaugcaaacg cacacucgua      840

gacagaggcu ggggcaacgg augcggacug uucggcaaag gcucacuggu aacaugcgcc      900

aaauucgcau gcagcaagaa aaugacaggc aaaagcaucc aaccagaaaa ccuagaauac      960

cgcaucaugc uaagcgucca cggaagccaa cacagcggca ugauagucaa cgacaccggg     1020

cacgaaacag acgaaaaccg agccaaaguc gaaaucacgc caaacagccc ccgcgccgaa     1080

gcaacacuag gcggcuucgg aagccucggc cuagacugcg aaccacgcac aggacuagac     1140

uucagcgacc uauacuaccu cacuaugaac aacaaacacu ggcuaguaca caaagaaugg     1200

uuccacgaca uaccccuccc uuggcacgcc ggcgccgaca ccgguacacc ccacuggaac     1260

aacaaagaag cccucguaga guucaaggac gcucacgcca aacgacaaac cgucgucguu     1320

cucggauccc aagaaggcgc aguacacaca gcacuagccg gcgcccuaga agcugaaaug     1380

gacggagcca aaggacgccu aucaucagga cauuuaaagu gccgccucaa aauggacaaa     1440

cuccgacuca aaggagucuc cuacagccug ugcacagcag ccuucacauu cacaaaaaua     1500

cccgcagaga cccugcacgg aaccguaacc guagaggucc aauaugccgg aaccgacggc     1560

cccugcaaag ucccagccca aauggcaguc gacaugcaaa cacucacccc cguaggaaga     1620

cuaaucaccg caaacccagu aaucaccgaa aguaccgaaa acagcaaaau gaugcuagaa     1680

cucgaucccc cauucggcga cagcuacaua gucaucgggg ucggcgaaaa gaaaaucacc     1740

caccacuggc accgcucagg aucaacaaua ggaaaagcau ucgaggcaac cguacgcggc     1800

gccaaacgca uggccguccu aggagacaca gcaugggacu uuggcuccgu aggaggcgcg     1860

cucaacagcc ucggaaaagg aauacaccaa aucuucgggg ccgccuucaa aucccuauuu     1920

ggaggaaugu cgugguucuc gcaaauccuc auaggcacac uccugaugug gcucgggcua     1980

aacaccaaaa acggcuccau cagucuaaug ugucucgccc ucggaggagu ccuaaucuuc     2040

cuaagcacag cuguaagcgc a                                               2061


<210>  2746
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) CDS opt

<400>  2746
accagcgucg gaauuguagg acugcuccua acgacagcaa uggcagccga aguaaccaga       60

agaggcuccg cauauuauau guaccucgac cggaacgacg ccggagaagc caucuccuuc      120

ccaaccaccu uagguaugaa caaaugcuac auccaaauca uggaccucgg acacacgugc      180

gacgcaacca uguccuacga augcccaaug cuagacgaag gcguagaacc agacgacgua      240

gacugcuggu gcaacaccac cucgacuugg guaguauacg gaaccugcca ccacaaaaag      300

ggcgaagcac gccgaucacg cagagccguc acccuccccu cacacagcac ccgaaaacug      360

caaacacggu cccaaaccug gcucgaaagc cgagaauaua cuaaacaccu cauaagagua      420

gagaacugga uuuucagaaa cccgggcuuc gcccucgccg cggcggcaau agcauggcuc      480

cuaggcuccu ccacaucgca aaaagugaua uaucucguca ugauacuacu caucgcccca      540

gcauacucca uacgaugcau cggcguaucc aauagagacu ucgucgaagg aaugagcgga      600

ggcacguggg uggacguagu ccucgaacac ggcggcugcg uaaccgucau ggcacaagau      660

aaaccaacgg uagacaucga acucgucaca accaccguau caaacauggc ugaaguccga      720

uccuacugcu acgaagccuc caucagcgac auggcauccg acucacgcug ccccacccaa      780

ggcgaggccu accuggacaa acaaucagac acccaauacg ucuguaagcg cacccuggug      840

gaccggggcu ggggcaacgg gugcggacuc uucggcaaag gcagccucgu aacaugcgca      900

aaauucgccu gcagcaagaa aaugacagga aaaagcaucc aacccgaaaa ccucgaauac      960

cgaauaaugc uaagcguaca cggaucccaa cacuccggua ugauaguaaa cgacacaggc     1020

cacgagaccg acgaaaaccg cgccaaaguc gaaauuaccc ccaauucgcc ccgagcagaa     1080

gcuacccuag gaggguuugg cucccuagga cuggacugcg aaccacgcac cggacuagac     1140

uucuccgacc uauacuaccu caccaugaac aacaaacauu ggcuaguaca uaaggaaugg     1200

uuccacgaca ucccacuacc cuggcacgcc ggagccgaca caggcacacc ccacuggaac     1260

aacaaagagg cacucguaga guucaaagac gcccacgcca aaagacaaac cgucgucguu     1320

cucggcucac aagaaggcgc cguacacacg gcccucgcug gugcccugga agccgaaaug     1380

gacggagcca aaggacgccu cuccagcggc caccuaaaau gccgccuaaa aauggacaaa     1440

cuccgccuca aaggcguauc cuacucacuc ugcaccgccg ccuucaccuu caccaaaaua     1500

cccgcagaaa cgcuccacgg cacaguaacc gucgaaguac aguacgcugg aacggauggu     1560

cccugcaaag uacccgccca aauggcagua gacaugcaaa cccucacccc cguaggccgc     1620

cucaucaccg ccaacccagu aaucacagaa ucaaccgaaa acucgaaaau gauguuagaa     1680

cucgacccac ccuucggaga cucauacauc gucaucggcg ucggcgagaa aaagaucacg     1740

caccacuggc accgcagcgg cucaacaauc ggaaaggccu ucgaagcaac cguaagagga     1800

gccaaacgca uggccguacu cggcgacacc gcaugggacu ucggcuccgu cggcggagca     1860

cuaaacuccc ucggcaaagg aauccaccaa aucuuuggcg cagcuuuuaa aucccucuuc     1920

ggcggcaugu caugguucuc ucaaauccuc aucggcacac uccuaaugug gcucggguua     1980

aacgcuaaaa acggcuccau cagccucaug ugccuagcac ucggcggagu acuaauauuc     2040

cuaucaaccg caguaucagc c                                               2061


<210>  2747
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) CDS opt

<400>  2747
acauccauag gcaucauagg auuacuacua accaccgcca uggccgccga aauaacacgc       60

cggggauccg cuuacuauau guaccucgac cgcagugacg ccggcaaagc uaucagcuuc      120

gcaaccacuc uaggaguaaa uaaaugccac guucaaauca uggaccuagg ccacaugugc      180

gacgccacaa uguccuacga augccccaug cuagacgaag guguagagcc ggacgacguc      240

gacugcuggu gcaauaccac cagcaccugg gucgucuacg gcacgugcca ccacaagaaa      300

ggagaagcac gucguucccg acgcgccguu acucugccaa gccacuccac ccguaaacuc      360

cagaccagau cacaaacaug gcuagagucc agagaauaca ccaaacaccu caucaaaguc      420

gagaacugga uauuccgaaa ccccggcuuc gcccucgucg ccgucgcaau cgccuggcua      480

cucggaucca gcacuucaca gaaagucauc uaccucguca ugauccuacu caucgccccg      540

gcuuacagca uccgaugcau cggagugagc aaccgugacu ucgucgaagg caugucuggc      600

gguaccuggg uagacgucgu ccuagaacac ggaggcugcg ucaccguaau ggcccaagac      660

aaacccacug ucgacauaga acucgucaca acaaccguca gcaauauggc agaaguccgc      720

uccuacugcu acgaggccuc cauaagcgac auggcaucag acucccgcug cccaacccaa      780

ggagaagccu accuggacaa acaauccgac acgcaauacg ucugcaaaag aaccuuaguc      840

gaccgcggau ggggaaacgg gugcggccua uucggcaaag gcucgcucgu aaccugcgcc      900

aaguucacau gcagcaaaaa gaugacgggc aaaagcauac aaccggaaaa ccucgaauac      960

agaauaaugc ucucggucca cggcucccaa cacagcggaa ugauuggaua cgaaacugac     1020

gaggacagag ccaaagucga gguaacccca aacuccccgc gcgcugaagc cacccuagga     1080

ggcuucggaa gccugggccu ugacugcgaa ccccgaacag gacuggacuu cucagaccug     1140

uacuacuuga ccaugaacaa caaacacugg cugguccaua aagaaugguu ccacgacauc     1200

ccccuuccau ggcaugcagg cgcagacacc ggcacacccc acuggaacaa caaagaagcc     1260

cucgucgagu ucaaggacgc acacgcaaaa cgucaaaccg uugucguccu aggcucacaa     1320

gaaggagcag uucacaccgc ccucgcgggc gcccuagagg cagaaaugga cggagccaaa     1380

gggcgccucu ucagcggcca ccuaaaaugc cgccuaaaaa uggacaaacu ccgccugaaa     1440

ggaguaucgu acucccucug caccgcagca uucaccuuca ccaaaguacc cgcggagacc     1500

cuacacggaa caguaaccgu ugagguccaa uaugccggaa ccgacggccc augcaagauc     1560

cccguacaga uggccguaga caugcaaaca cugacaccag ucggacgccu aaucaccgca     1620

aaccccguga ucacagaguc caccgaaaac agcaaaauga ugcucgagcu cgacccaccu     1680

uucggcgaca gcuacaucgu cauaggaguc ggagacaaga aaauaaccca ccacuggcac     1740

cgaagcgggu ccacgaucgg caaagccuuc gaagcgacag uuagaggagc caaacguaug     1800

gccguacuug gcgacaccgc cugggacuuc ggaagugucg gcggcgucuu caacucacuc     1860

ggcaaaggaa uccaccaaau cuucggagcc gcguucaaau cacuauucgg aggaaugucc     1920

ugguucuccc aaauccuuau aggaacacuc cugguauggc uaggucuaaa caccaaaaac     1980

ggcagcauau cccuaaccug ccucgcgcuc ggcgguguca ugauauuccu cucuaccgcc     2040

gucuccgcc                                                             2049


<210>  2748
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) CDS opt

<400>  2748
accaguguag gcaucgucgg gcuccuacuc accacggcca uggcggcaga ggucacccga       60

cguggauccg cauauuacau guaccucgac cgaaacgacg ccggggaagc gauauccuuc      120

ccgaccaccc ucggaaugaa caaaugcuac auacaaauca uggaccuagg acacaugugc      180

gacgcaacaa ugagcuacga augcccaaug cucgacgaag gcguggaacc agacgacguc      240

gacugcuggu gcaacaccac cuccacaugg guaguauacg gcacaugcca ucacaagaaa      300

ggcgaagccc gacgguccag acgcgcugug acacucccaa gccacagcac aagaaaacuc      360

caaacacgcu cccagaccug gcucgaaagc cgggaauaua caaaacaccu cauacgcguc      420

gaaaacugga uauuccgcaa cccgggguuc gcacucgcgg ccgccgccau cgcauggcuc      480

cuuggcagca gcacuucaca aaaagugaua uaccucguaa ugauccuccu gaucgcgccc      540

gccuacucca uccgcugcau aggcgucagc aaccgcgacu ucguggaagg aauguccggc      600

ggcacguggg ucgacgucgu acucgaacac ggaggcugcg ucacaguaau ggcccaagac      660

aaaccaacgg ucgacaucga acuuguaacc accacaguaa gcaacauggc cgaaguccgc      720

ucauacugcu acgaggcaag cauauccgac auggcaucag auucacgcug ccccacccaa      780

ggagaagccu accuggacaa acaaucagac acacaauacg uaugcaaacg gacccucgua      840

gaccgcggcu ggggcaacgg cugcggacuc uuuggcaaag guucgcucgu aaccugcgcc      900

aaauucgccu gcuccaaaaa gaugaccgga aagucaaucc aacccgagaa ccucgaauac      960

cgaaucaugc ucucggucca cgggucccaa cacaguggca ugaucgucaa cgacacggga     1020

cacgaaacag acgagaacag agccaaagua gaaaucaccc ccaacucccc aagagcggaa     1080

gcaacacucg gcggcuucgg cagccuuggc cuagacugcg aaccacgaac cggacuggac     1140

uucagugacc ucuauuaccu aacaaugaac aauaaacacu ggcucguaca caaagaaugg     1200

uuccacgaca uaccgcuacc cuggcacgcg ggugccgaca ccggaacccc acacuggaau     1260

aacaaggaag cccuagucga auuuaaagac gcacacgcca agagacaaac agucguagua     1320

cuaggcucac aagaaggcgc aguacacaca gcccuagcag gagcgcucga agccgaaaug     1380

gacggcgcga aaggccgacu gagcagcggc caccuaaaau gccgacuaaa aauggacaaa     1440

cuacgccuua aaggaguguc auacagccuc ugcacagcgg cauucaccuu caccaagaua     1500

ccugcagaaa cacuucacgg caccguaacc gucgaaguac aauaugccgg aacagacggc     1560

ccaugcaagg uacccgcgca aauggccguc gacaugcaaa cccuaacccc cguaggacga     1620

cuaaucacag caaaccccgu caucaccgaa ucgacagaaa acucaaaaau gaugcuagaa     1680

cuagaucccc cauucggcga cucauacaua gucauaggcg ucggcgaaaa gaaaaucaca     1740

caccauuggc accgauccgg aagcacaaua ggaaaagcau ucgaggcaac aguaagaggc     1800

gccaaacgca uggcaguccu cggcgacaca gcaugggacu ucggcagcgu aggaggagca     1860

cuaaacuccc ugggaaaagg aauacaccaa auauucggcg ccgcauucaa aagccucuuc     1920

ggagggauga gcugguucuc ccaaauccua aucgggaccc uccugaugug gcuaggccua     1980

aacaccaaaa acgggagcau cagccucaug ugccuagcac uaggcggcgu ccuaauauuc     2040

cuaucaaccg caguuagcgc a                                               2061


<210>  2749
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) CDS opt

<400>  2749
ucacaaaaag ucauauaccu ggucaugauc cuacucaucg ccccagccua cagcauacgc       60

ugcauaggag uaucgaaccg agacuuuguc gagggcauga gcggaggcac augggucgac      120

aucguccucg aacacggcgg gugcgugaca guaauggcac aagacaaacc gacaguggac      180

auagagcuag ucacaaccac ggucucgaac auggcagaag uaaggagcua cugcuacgaa      240

gccagcauaa gcgacauggc aucagacuca aggugcccaa cacaaggcga ggcuuaccuc      300

gacaaacaaa gcgacaccca auacgugugc aaacgcaccc uaguagaccg cgguuggggc      360

aacggaugcg gccuguucgg caaagggagc cuagucacau gcgcgaaauu cgccugcagc      420

aagaaaauga cuggaaaaag cauccagccg gaaaaucucg aauaccgcau aaugcucagc      480

gugcacggga gccaacacuc aggaaugauc gugaacgaca ccggacacga aacagacgaa      540

aacagagcga aaguagaaau aaccccaaac ucaccaagag ccgaagccac acuaggagga      600

uucggcucgc uaggccuuga cugcgaaccc cgaaccgggc uagacuucuc ggaccucuau      660

uaccucacca ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauaccg      720

cuacccuggc acgcaggcgc agacacaggc acaccccacu ggaacaacaa agaggcccuc      780

guagaauuua aagacgccca cgcaaaacgc caaacagugg ucguacuagg gagccaagaa      840

ggggcaguuc acacggcccu agccggagcc uuagaggcgg aaauggacgg agcgaaagga      900

cgccucucga gcggccaucu aaaaugucgc cugaaaaugg acaaacucag gcucaaaggc      960

guauccuaca gccuauguac cgccgcguuc acauucacaa aaauacccgc agaaacgcua     1020

cacggcacag uaacagucga gguacaauac gcaggcacag acgggcccug caaaguaccc     1080

gcgcaaaugg cgguagacau gcaaacccua acacccgucg gcagacuaau aaccgcaaac     1140

ccaguaauca cagagagcac agagaacagc aaaaugaugc ucgagcucga cccaccguuc     1200

ggagacucau acauagucau aggcgugggc gaaaagaaaa uaacgcacca cuggcaccgc     1260

uccggaucaa ccauaggcaa agccuucgaa gccaccguac gaggcgccaa aagaauggcc     1320

gugcucggag acacagccug ggacuucggc ucg                                  1353


<210>  2750
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) CDS opt

<400>  2750
ucccaaaaag ucauauaccu aguaaugaua cuacucauag ccccagcaua cagcauccgc       60

ugcaucggcg uaucaaacag ggacuucgua gaaggcauga gcggaggaac auggguagac      120

guaguccuag aacacggcgg augcgugacc guaauggcac aagacaaacc gaccguagac      180

auagagcuag ucaccacaac cgucuccaac auggcagaag ucagaagcua cugcuacgaa      240

gcaagcauca gcgacauggc cagcgacagc cggugcccga cccaagggga agccuaccua      300

gacaaacaga gcgacacaca auacgugugc aaacgcacac ucguagaccg cggauggggc      360

aacggcugcg gacuguucgg caaaggaagu cuagucacgu gcgcaaaauu cgccugcagc      420

aagaaaauga ccggcaaauc gauccaaccc gaaaaccucg aauaccgcau caugcuaagc      480

gugcacggca gucagcacuc ggguaugauc gugaacgaca caggacacga aacagacgaa      540

aaucgcgcaa aaguggaaau aaccccaaac ucccccagag ccgaggccac ccucggaggc      600

uucggcucgc ucggacucga cugcgaaccc cggaccggcc ucgacuucuc cgaccuguac      660

uaucuaacga ugaacaauaa acacuggcuc guacacaaag aaugguucca cgacauaccc      720

uuacccuggc acgccggcgc agacacagga acgccacacu ggaacaacaa agaagcccuc      780

guggaguuca aagacgcaca cgccaagcgc caaaccgucg uaguacuagg aucccaagaa      840

ggcgccguac acacggcccu agccggagcc cuggaagcgg aaauggacgg agcaaaaggc      900

agacucagca gcggacaccu gaaaugccga cugaagaugg acaaacuaag acucaaaggc      960

gugagcuaca gccucugcac agccgcauuc acguucacaa agauacccgc agaaacacua     1020

cacggcacag uaacagucga aguccaauac gccggcacgg acgggcccug caaaguaccc     1080

gcacaaaugg caguggacau gcaaacgcuc acacccgugg gaagacuaau aacagcaaac     1140

ccagucauaa cagaaagcac agaaaacagc aaaaugaugc uagaacucga cccacccuuc     1200

ggcgacucgu acauagugau aggcguagga gaaaagaaaa uuacacauca cuggcaccgc     1260

ucaggcagca caauaggcaa agccuucgaa gcaacggucc gcggagcaaa acgcauggcc     1320

gugcucggag acaccgccug ggacuucgga uca                                  1353


<210>  2751
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) CDS opt

<400>  2751
ucgcaaaaag uaaucuaccu agucaugauc cuacuaaucg cccccgccua cuccauacga       60

ugcaucggag uaagcaacag agacuucguc gaaggaauga gcggcggaac cugggucgac      120

guaguccugg aacauggcgg cugcgucacc gucauggccc aagacaaacc caccguagac      180

auagaacucg ucacaacaac aguauccaac auggcugaag uccgaagcua cugcuacgaa      240

gccucaauaa gcgacauggc cucagacagc agaugcccca cccaaggaga agcauaccua      300

gacaaacaaa gcgauacaca auacguaugu aaacgcacac uggucgaccg cggaugggga      360

aacggcugcg gccucuucgg caaaggcagu cucguaaccu gcgccaaauu cacaugcagc      420

aagaaaauga ccggaaaauc cauacaaccu gaaaaccucg aauauagaau caugcuaucg      480

guccacggaa gccaacauuc gggcaugauc ggcuacgaaa ccgacgaaga ccgcgccaaa      540

guagaaguca caccgaacag uccccgagca gaagccaccc uaggcggcuu cggaucccuc      600

ggccuagacu gcgaacccag aaccggauua gacuucagcg acuuauacua ccucaccaug      660

aacaacaaac acuggcucgu ccacaaagaa ugguuccacg acauaccccu accauggcac      720

gccggcgcag acaccggcac cccgcacugg aacaacaaag aagcccuagu cgaguucaaa      780

gacgcacacg ccaaacgcca aaccguaguc guacucggaa gccaagaagg cgcaguacac      840

accgcccugg ccggagcccu cgaagccgaa auggacggcg ccaaagggag acucuucagc      900

ggacaccuaa aaugcagacu caaaauggac aaacuccgcc uaaaaggcgu guccuacucc      960

cuaugcacag cagccuucac guucacaaaa gugccagccg aaacacuaca cggcacagua     1020

accgucgaag uccaauacgc gggaacagac ggcccaugca aaaucccagu ccaaauggca     1080

gucgacaugc aaacacucac ccccgucgga cgacuaauca ccgccaaccc agucaucacc     1140

gaauccacag aaaacucaaa aaugaugcuc gaacucgacc cacccuucgg agacuccuac     1200

aucgucauag gagucggcga caaaaagauc acccaccacu ggcacagaag cggcagcacc     1260

auaggcaaag ccuucgaagc aacaguacgc ggagcaaaac gcauggccgu ccuaggcgac     1320

accgccuggg acuucggguc c                                               1341


<210>  2752
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) CDS opt

<400>  2752
agccaaaaag ucauauaccu cguaaugauc cuacucauag ccccggccua cagcauccgc       60

ugcaucggag uaucaaacag agacuucgua gaaggcauga gcgggggaac auggguagac      120

guaguccucg aacacggagg augcguaaca guaauggcac aagacaaacc gaccguagac      180

auagaauuag ucacaaccac agucucaaac auggccgaag uccgcagcua cugcuacgaa      240

gcgagcauaa gcgacauggc aagcgauuca agaugcccca cccaggggga agccuaccua      300

gacaaacaaa gcgacaccca guacguaugc aaacgaaccc ucguggaccg cggaugggga      360

aacgguugcg gccuauucgg gaaagggagc cuagucacau gcgcaaaauu cgcaugcagc      420

aagaaaauga ccgggaaaag cauacaacca gaaaaccuag aauaccgcau caugcugagc      480

guacacggga gccaacacuc aggaaugauc gucaacgaca ccggacacga aacagacgaa      540

aaccgagcca aaguagaaau aaccccuaac ucaccacgag ccgaagcgac acugggcgga      600

uucggcucgc ucggacuuga cugcgaaccc cggaccggac uagacuucuc ggaucuauac      660

uaccucacga ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauaccc      720

cuacccuggc acgcgggcgc cgacacagga acaccccacu ggaacaacaa agaagcacuc      780

guagaguuca aagacgcaca cgccaaacgc caaaccguag ucguacuagg aagccaagaa      840

ggcgccgucc acacggcacu agccggagcc uuagaagccg aaauggacgg cgccaaaggc      900

cggcuaucaa gugggcaccu gaaaugcaga cugaaaaugg acaaacucag gcucaaagga      960

gugagcuaca gccugugcac ggcagcauuc acauucacaa aaauaccagc ggaaacacuc     1020

cacgggaccg ucacggucga aguccaauac gcaggcacag acggacccug caaaguaccc     1080

gcgcaaaugg ccgucgacau gcaaacgcuc acaccaguag gcaggcucau aacagcaaac     1140

ccaguaauua ccgaaagcac agaaaacagc aaaaugaugc uagaacucga cccaccauuc     1200

ggagacucau acauagucau cggcguagga gagaagaaaa uaacacacca cuggcaccga     1260

ucaggcucca caaucggcaa agccuucgag gcaaccguca gaggagccaa acgcauggca     1320

gugcucggag acacggccug ggacuucggc ucg                                  1353


<210>  2753
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) CDS opt

<400>  2753
acaagcguag gcauagucgg cuuacugcuc acgaccgcca uggcagcgga aguaaccaga       60

cgaggaagcg cauacuauau guaucuggac cgaaacgacg ccggcgaagc aaucagcuuc      120

cccacaacgc ucggcaugaa caaaugcuac auacagauca uggaccucgg gcacaugugc      180

gacgccacga ugaguuacga gugccccaug cucgacgagg gagucgaacc agacgacguc      240

gacugcuggu gcaacaccac cagcaccugg gugguauacg gcacaugcca ccacaagaaa      300

ggagaagcac gccgcucucg acgagccgua acacuccccu cccacagcac cagaaaacuc      360

cagacaagau cacaaacaug gcuagaauca cgcgaauaca caaaacaccu cauaaggguc      420

gaaaacugga uauucagaaa ucccggauuc gcccucgccg ccgccgcgau cgcaugguua      480

cucggcuccu ccaccagcca aaaagucaua uaccuaguga ugauacuccu caucgcucca      540

gcauacucca uccggugcau aggaguaucc aaccgagauu uuguggaggg aaugucggga      600

ggcaccuggg ucgacaucgu ccucgagcac ggaggcugcg uaacagucau ggcacaagac      660

aaacccacag ucgacauaga gcuaguuacc acaaccguau ccaacauggc cgaaguaaga      720

ucguacuguu acgaagcaag caucagcgac auggccagcg acagccgcug cccuacccaa      780

ggcgaagccu accucgacaa acaguccgac acacaauacg uauguaaacg cacccuaguc      840

gacagagguu gggguaacgg cugcggacuc agcggcaaag gcagccucgu gaccugcgcg      900

aaauucgccu gcucaaagaa aaugaccggc aaauccauac aacccgaaaa ccuagaauac      960

cgcaucaugc ucucggucca cggaucacag cacucaggca ugauagucaa ugacacagga     1020

cacgaaaccg acgaaaaccg agcuaaaguc gaaaucaccc caaacagccc acgcgccgaa     1080

gccacgcucg gaggcuucgg aucacuaggg cuagacugcg aaccccgcac aggacuagac     1140

uucagcgacc ucuacuaccu gacaaugaac aacaaacacu ggcucguuca caaagaaugg     1200

uuccacgaua ucccccuacc cuggcacgca ggagccgaca caggaacccc acacuggaac     1260

aacaaggaag cccucguaga guucaaagac gcacacgcca agcgacaaac cgucguagua     1320

uuaggcucgc aagaaggcgc aguacacaca gcccucgccg gcgcacucga agcagaaaug     1380

gacggcgcca aagggcgacu aaguagcggc caucucaaau gccgacucaa aauggacaaa     1440

cuccgacuaa aaggcgucuc auacucccua ugcaccgcag cauucaccuu caccaaaauc     1500

ccggccgaaa cacuccacgg aaccgucacc gucgaaguac aauacgcagg aacggauggc     1560

ccguguaaag uucccgcgca aauggccguc gacaugcaaa cgcuuacacc agucggcaga     1620

cucauaacag ccaacccagu aauaaccgaa agcacagaaa auagcaagau gaugcucgaa     1680

cuagacccac ccuucggaga cuccuacaua guaaucggcg uaggagaaaa gaaaaucacc     1740

caccacuggc accgaagcgg aagcacgauc ggcaaagcau uugaagccac cgugcgcggc     1800

gccaagagaa uggccguacu cggcgacaca gccugggacu ucgguuccgu cggaggcgca     1860

cuaaacucac ugggcaaagg cauacaccaa aucuuuggag cagccuucaa aagccucuuc     1920

ggaggcaugu caugguucuc ccagauacuc aucggcaccc uacucaugug gcuaggccug     1980

aacaccaaga acgggucaau aucacuaaug ugccuagccc ucggcggagu acuaauauuc     2040

cugagcaccg ccguaagcgc a                                               2061


<210>  2754
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) CDS opt

<400>  2754
acgagcgucg gcaucgucgg acugcuucuc acaaccgcga uggcagccga aguaacccgc       60

agaggauccg cauacuacau guaccuagac cgaaacgacg ccggagaagc caucagcuuc      120

ccaaccaccc ugggaaugaa uaaaugcuac auacagauca uggaccuggg ccauaccugc      180

gacgcgacca uguccuauga gugcccaaug cuagacgaag gaguagagcc cgaugacguc      240

gacugcuggu gcaacacaac uucgaccugg gucguguacg gaaccugcca ccacaagaaa      300

ggcgaagcac ggcgaucaag acgagccguc acacuaccaa gccacagcac ccgcaagcua      360

caaacacgaa gccagacaug gcuagaaucc cgagaguaca ccaaacaccu aauccgagua      420

gagaacugga ucuuccgcaa cccaggcuuc gcacuugccg cggccgcaau cgccuggcug      480

cucggcagca gcacaaguca aaaggucauc uaccugguca ugauacuccu aauagcgccg      540

gccuacagca uccguugcau cggcgucucc aaccgagacu ucguugaagg caugucagga      600

ggcacauggg uagacguagu acucgagcac ggaggcugcg uaaccgugau ggcgcaggac      660

aaaccaacag uagacaucga gcuagucacc acaaccgugu cgaacauggc ugaagucagg      720

agcuacugcu augaagcauc aauaucagac auggcaagcg acuccagaug ccccacacaa      780

ggcgaagccu accucgacaa acaaucugac acccaauacg ugugcaaaag gacccuagua      840

gaccggggcu ggggcaacgg gugcggccua agcggcaaag gcagccuagu aaccugcgcc      900

aaauucgcau gcuccaagaa aaugacaggc aaauccaucc aaccagaaaa ccuagaauac      960

agaaucaugu uaucagucca cggaagccaa cacucaggca ugaucgucaa cgacaccgga     1020

cacgaaacag acgaaaaccg cgcgaaagua gaaauaaccc caaacucacc cagagcagaa     1080

gccacacugg gcggcuucgg cucccucgga cuggacugcg aaccccgaac cgggcuagac     1140

uucucagacc uauacuaccu cacaaugaac aacaaacacu ggcucgucca caaagaaugg     1200

uuccacgaca ucccacuccc cuggcacgca ggcgccgaca ccggcacacc acacuggaau     1260

aacaaagaag ccuuagugga guucaaagac gcacaugcca aaagacaaac cgucgucgua     1320

cuaggcaguc aagaaggcgc aguacacaca gcgcucgcgg gcgcgcugga agccgaaaug     1380

gacggagcaa aaggcagguu aucaagcggc caccucaaau gccgacuaaa aauggauaaa     1440

cuacgccuca aaggcguaag cuacagccuc ugcacagcag cauucaccuu caccaaaaua     1500

cccgccgaaa cacugcacgg cacaguaaca gucgaagucc aauacgcagg caccgacgga     1560

ccaugcaaag uccccgccca gauggccguc gacaugcaaa cccucacucc cguagggcgc     1620

uuaaucaccg ccaacccagu caucacagaa uccacagaaa acaguaaaau gaugcucgaa     1680

cucgacccgc cuuucggaga cuccuacauc guaaucggcg uaggcgaaaa gaagaucacc     1740

caucacuggc acagaucagg uagcaccauc ggcaaagcgu ucgaagcaac cgugcgcggc     1800

gcgaaacgca uggcaguucu cggcgacacu gcaugggacu uuggcucagu aggaggcgca     1860

cucaacagcc uaggaaaagg aauacaccaa auauucggcg ccgcauucaa aagccuauuc     1920

ggcggaaugu caugguucag ccaaauacuc auuggcaccc uccucaugug gcugggccuc     1980

aacgccaaaa acggcuccau aucgcucaug ugccucgcac uaggcggcgu auuaauauuc     2040

cucucaacag cugucagcgc a                                               2061


<210>  2755
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) CDS opt

<400>  2755
accuccauag gcauaaucgg ccuacugcuu acgacagcaa uggccgcaga aauaacacga       60

cggggcuccg ccuacuacau guaccucgac cgcucggacg ccggaaaagc aaucaguuuc      120

gcuacaaccc ucggcguaaa caaaugccac guccaaauca uggacuuagg ccacaugugc      180

gacgcgacua uguccuacga augccccaug uuagacgagg gcgucgaacc cgacgacguc      240

gacugcuggu gcaacaccac cucgacaugg guagucuacg gcacuugcca ucacaagaaa      300

ggagaagcac gccguucaag acgagcaguc acccuaccca gccacagcac acgaaaacua      360

caaacccgcu cccaaaccug gcuagaaagc cgcgaauaca ccaaacaccu cauaaaaguc      420

gaaaacugga uauuccgaaa ccccggcuuc gcacucgucg ccguagccau cgcauggcuc      480

cuaggaucca gcaccagcca gaaagucaua uaccuaguca ugauucuacu caucgcaccg      540

gcauacucca uacgcugcau cggaguaagc aacagagacu ucgucgaagg uauguccggc      600

ggcacuuggg ucgacgucgu ccucgaacac ggaggaugcg ugacagucau ggcacaagac      660

aaaccaacag uggacaucga acucguaacc accaccguca gcaacauggc cgaaguccga      720

agcuacugcu acgaagccag caucagcgac auggcuucag acagccgcug cccaacgcaa      780

ggggaagccu accucgacaa acaaucagac acccaauacg uaugcaaacg cacccugguc      840

gaucgcggau ggggcaacgg gugcggccuu ucuggcaagg ggucccuagu aaccugcgca      900

aaauucaccu guucaaagaa aaugaccggc aaauccaucc aaccugaaaa ccucgaauac      960

cguauaaugc ucagugucca cggaucccaa cacagcggaa ugaucggcua cgaaacagac     1020

gaagaccgcg ccaaaguaga aguaaccccc aacucgccac gagccgaggc cacacuaggc     1080

gguuucggcu cccuaggacu cgacugcgaa ccucgcacag ggcucgacuu cuccgaccua     1140

uacuaccuga caaugaacaa caaacacugg uuaguccaua aggaaugguu ccaugacaua     1200

ccccuccccu ggcacgcugg cgccgacaca ggcacaccgc acuggaacaa caaagaagcu     1260

cuaguagaau uuaaagacgc ccacgccaaa cgacaaacag ucguuguccu aggcucccaa     1320

gagggagcag uacacacagc ccuagcgggc gcccuagaag ccgaaaugga cggcgccaaa     1380

ggccgccucu ucuccggcca ccucaaaugu cgccucaaga uggacaaacu ccgccuaaaa     1440

ggcguaucuu auagccuaug cacagcagca uucacauuca caaaaguacc agccgaaacg     1500

cuccacggaa cggucacagu cgaaguccaa uacgccggca ccgacggacc gugcaagauc     1560

ccaguacaaa uggcaguaga caugcagacc cuaacaccag ucggccgccu gauaacugcc     1620

aacccaguca uaaccgaauc gaccgaaaac ucaaagauga ugcuugagcu ugacccaccc     1680

uucggcgacu ccuacauugu aaucggcguc ggggacaaga aaaucaccca ccacuggcac     1740

agaagcggau ccaccauagg aaaagccuuc gaagcaaccg uaagaggagc caaaagaaug     1800

gcaguccucg gcgauacagc uugggacuuc ggaagcgucg gaggcgucuu caacucccuc     1860

gguaaaggca uccaccaaau auucggcgca gcauucaagu cccuguucgg cggaauguca     1920

ugguucucac agauacuaau cggaacacug cuaguauggc uaggccucaa caccaaaaac     1980

ggcagcauca gccuuacgug ccuagcacug ggcggaguca ugaucuuucu cuccaccgcc     2040

guauccgcc                                                             2049


<210>  2756
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) CDS opt

<400>  2756
acaucaguag ggauaguagg acuccuccug acaacagcca uggccgccga agucacaaga       60

cguggcagcg cguacuacau guaccuggau agaaacgacg caggcgaagc aaucaguuuc      120

cccacaacac ugggcaugaa caagugcuac auccaaauca uggaccuggg ccacaugugc      180

gaugccacca ugagcuacga augccccaug cuagacgaag gcgucgaacc cgacgacguc      240

gacugcuggu gcaacacaac aaguacgugg gucguguacg gcacauguca ccacaaaaag      300

ggagaagcuc ggcggucccg ccgagccguc acgcucccca gccacagcac aagaaaacuc      360

caaacacgaa gccaaacaug gcuagaauca agagaauaca ccaaacaccu cauaagagug      420

gaaaacugga ucuucagaaa cccaggcuuc gcacuagccg cagccgcaau cgcgugguua      480

cuuggcucca gcacuucuca gaaagucaua uaccugguaa ugauacuacu aauagccccg      540

gcauacucga uacgaugcau cgggguaagu aacagggacu ucgucgaagg aauguccgga      600

ggcacauggg uagacguagu ccuagaacac ggcggaugcg uuacgguaau ggcccaagac      660

aaaccaaccg uagacaucga acuggucaca acaaccguaa gcaacauggc agaggucaga      720

agcuacugcu acgaagccuc aauaagcgac auggcaagcg auucccggug cccaacucaa      780

ggggaagcau accuagacaa acaauccgac acacaauacg uaugcaaacg cacacuugua      840

gacagaggcu gggggaacgg cugcggacua uccggaaaag gcucguuagu gacaugugca      900

aaauucgcau gcagcaagaa aaugacaggg aaaucaauac aacccgaaaa ccuagaauau      960

cggaucaugc ucuccguaca cggcucacaa cacagcggaa ugaucguaaa cgacaccgga     1020

cacgaaacag augaaaaccg agcuaaagua gaaaucaccc ccaacagccc aagagcagaa     1080

gcaacacucg gaggauucgg uucacuugga cucgacugcg agcccagaac cggccucgac     1140

uucucagacc uauacuaccu aacgaugaau aacaaacacu ggcucguaca caaagaaugg     1200

uuccacgaca uaccccugcc guggcacgca ggggcggaca caggaacccc acacuggaac     1260

aacaaagaag cgcuggucga guucaaagac gcccacgcua aacgccaaac cguaguagua     1320

cucggaagcc aggaaggagc cguacacacc gcccuggccg gggcacucga agcagaaaug     1380

gacggcgcca aaggccgccu aagcagcgga caccuaaaau gccgucugaa aauggacaaa     1440

uugcgacuca agggaguauc cuacucccuu ugcaccgcag cauucacauu cacaaaaaua     1500

cccgccgaaa cacuacaugg cacaguaaca guagaggucc aauacgcagg cacggacggu     1560

ccaugcaaag uacccgcgca aauggccguc gacaugcaaa cacugacucc agucggcaga     1620

cucauaaccg caaaccccgu aauaaccgaa aguaccgaaa acagcaaaau gauguuagag     1680

cuagacccac ccuucggcga cagcuacaua guaauaggag ucggcgagaa aaagauaacc     1740

caccacuggc accgcagcgg aagcaccauc ggcaaagccu ucgaagccac aguccgcggg     1800

gccaaacgaa uggcaguccu gggcgacaca gcaugggacu ucgggucagu cggcggcgca     1860

cuaaacagcc ucgggaaagg cauacaccaa aucuucggug cagcuuucaa aagccuauuc     1920

ggugggaugu caugguucag ccaaauacuc auaggcaccc uccucaugug gcugggccuc     1980

aacaccaaaa acggcucaau aagccuaaug ugccuagcac uaggcggcgu ccugauauuc     2040

cuguccacag ccguauccgc c                                               2061


<210>  2757
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) CDS opt

<400>  2757
accagcgucg gaauagucgg acuccuccuc acaacagcaa uggccgcgga agucacccga       60

cgcggcagcg cuuacuacau guaccucgac cgaaacgacg ccggcgaagc caucagcuuc      120

cccaccaccc ugggcaugaa caaaugcuac auacaaauaa uggaccuugg ccacaugugc      180

gacgcgacca ugagcuacga augccccaug cucgacgaag gaguggagcc agacgaugua      240

gacuguuggu gcaacacaac aucaaccugg gucgucuacg gcacaugcca ccacaagaag      300

ggagaagcca gacgcagccg cagagcagua acccuaccgu cccacucuac cagaaaacuc      360

caaacacgaa gccagacaug guuagaaagc cgcgaauaca caaaacaccu caucagaguc      420

gagaacugga uauucagaaa cccaggcuuc gcauuagccg cagccgcaau cgccuggcuc      480

cuagggagcu caaccucaca aaaaguaaua uaccuaguca ugauacuacu cauagccccg      540

gccuacagca uacgaugcau aggugucuca aacagggacu ucgucgaagg caugagcgga      600

ggcacguggg uggacauagu acucgaacac ggcggcugcg ugaccgucau ggcacaagac      660

aaaccgacag uagacauaga auuggugaca accacagucu ccaacauggc cgaagugcgu      720

ucauacugcu acgaagcaag caucagcgac auggccucag acagccgaug cccaacccaa      780

ggcgaagccu accuagacaa gcaaagcgac acacaauacg ucugcaagcg gacgcuagug      840

gaccgaggcu ggggcaacgg cugcggacua uucggcaaag gaagccucgu aacaugcgcg      900

aaauucgcgu gcagcaagaa aaugaccggc aaauccaucc aacccgaaaa ccucgaauac      960

agaauuaugc ucuccguaca cggcagccaa cacuccggca ugauuguaca agauacaggc     1020

cacgagacag acgaaaacag agcgaaaguc gaaauaaccc ccaacucgcc ccgggccgaa     1080

gcaacucuag gaggauucgg aucgcuaggg cuugacugcg aaccccgaac aggacuagac     1140

uucuccgacc uguacuaccu aacaaugaac aacaaacacu ggcuaguaca caaagaaugg     1200

uuccacgaca uccccuuacc cuggcacgca ggcgccgaca caggaacccc acacuggaac     1260

aacaaagaag ccuuagugga guucaaagac gcacacgcaa aacgccaaac cguaguagua     1320

cuaggaagcc aagaaggagc aguacacaca gccuuggccg gagcguuaga agccgaaaug     1380

gacggggcaa aaggcaggcu uagcucggga cacuuaaaau gcagacuaaa aauggacaag     1440

cuccgccuga aaggaguauc cuacucccua ugcacagccg cguucacauu cacgaaaaua     1500

cccgccgaaa cuuuacacgg cacggucaca gucgaggucc aauacgcagg caccgacggg     1560

ccaugcaaag uacccgccca aauggccguc gauaugcaaa cccuaacccc aguaggaagg     1620

cuuauuaccg ccaacccagu cauaacagaa agcacagaaa acucaaaaau gaugcuagaa     1680

cuagauccac ccuucggaga cuccuacauc gucauaggcg uuggagaaaa gaaaaucaca     1740

caccacuggc acagaucagg aagcaccaua ggcaaagccu uugaagcgac aguacgcggc     1800

gcaaagagaa uggccguacu uggcgacaca gccugggacu ucggcucagu cgggggcgcg     1860

cugaacagcc uaggcaaagg gauacaccaa auauucggag cagcauucaa aucccucuuc     1920

ggcggaaugu caugguucuc acaaauccuc aucggaaccc uacugaugug gcuaggccua     1980

aacaccaaaa acggcagcau cucacucaug ugccuggccc uaggcggagu ccucaucuuc     2040

cucuccaccg ccguaucggc c                                               2061


<210>  2758
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) CDS opt

<400>  2758
accagcguag gaaucgucgg gcuacuacuc acaacagcaa uggcggccga ggucacccgc       60

agaggauccg ccuacuacau guaccuagac cgaaacgacg caggcgaagc gaucagcuuc      120

ccaacuacac uaggaaugaa caaaugcuac auacaaauaa uggaccuugg acacacaugc      180

gacgccacca ugagcuacga augcccaaug cuggacgagg gaguugagcc cgacgaugug      240

gacugcuggu gcaacaccac gagcacgugg guugucuaug gcacaugcca ccacaagaaa      300

ggcgaagcca gacgcagccg acgcgcagua acacucccau cacacucaac aagaaaacuu      360

caaacaagaa gccaaaccug gcuggaaagc cgagaauaca caaaacaccu gauacgaguc      420

gaaaacugga ucuuccggaa ccccggauuc gcacucgcgg cugcagccau cgccuggcua      480

cucggcucca gcacauccca aaaagucauc uaccucguga ugauauuauu gauugcaccc      540

gcauacucaa ucagaugcau cggcguaagc aaucgugauu ucguggaggg gaugucaggc      600

ggcaccuggg uggacgucgu acuagaacac gggggaugcg ucacaguuau ggcccaagac      660

aaaccaacag ucgacaucga acugguaacc acaaccguaa gcaauauggc cgaggugcga      720

ucauacuguu acgaggccag cauaagcgac auggcuucag acucccgcug ccccacccaa      780

ggagaagcgu accuagacaa acaaucagac acacaauacg uaugcaaaag gacccuaguc      840

gaccgaggau ggggcaacgg augcgggcua uucggcaagg gcagccuagu aacuugcgcc      900

aaauucgcau gcuccaagaa aaugacaggc aaaagcauac aaccagaaaa ccuagaauac      960

agaaucaugc uaucgguaca cggaagccaa cacagcggga ugauaguaca agacacggga     1020

cacgaaacag acgaaaacag ggccaaagua gagauaacgc ccaacagucc ccgggcagaa     1080

gcaacacuag gcggcuucgg aucacuaggc cuagacugcg aaccacgcac aggccuagac     1140

uucagcgacc ucuacuaccu caccaugaac aacaaacacu ggcugguaca uaaagaaugg     1200

uuccacgaca uaccguuacc guggcacgca ggcgcagaca ccggcacacc ccacuggaac     1260

aacaaagagg cccucguaga guuuaaagac gcccacgcaa aacggcaaac agucguaguc     1320

cuaggaucgc aagagggcgc aguccacaca gcacuagcag gcgcccuaga agccgaaaug     1380

gacggagcca aaggacgacu cuccuccggc caccuaaaau gcagacucaa gauggauaaa     1440

cuccgacuaa aaggcgucuc cuacucgcuc ugcacagcgg cauuuacauu caccaaaaua     1500

ccagccgaaa ccuuacacgg aaccguuacc gucgaaguac aguacgcagg aacagacggg     1560

ccaugcaaag ucccggcaca gauggccguc gacaugcaaa cccucacccc agucggccga     1620

cucaucacgg ccaaccccgu gaucacagaa ucgaccgaaa auucaaaaau gaugcuagaa     1680

cuugacccac ccuucggaga cagcuacauc gucauaggcg ucggcgagaa gaaaaucacc     1740

caucacuggc accgcagcgg aagcaccaua ggaaaggccu ucgaggcaac cgugagagga     1800

gcgaaacgaa uggccguacu cggcgacacu gcaugggacu ucggcuccgu cggcggagcu     1860

cuaaacuccc ugggaaaagg aauccaccaa auauucggag ccgccuucaa aagccuauuc     1920

ggaggaaugu ccugguucuc acaaauacua auaggcaccc uccugaugug gcuaggacua     1980

aacgcaaaga acggguccau aagccugaug ugccuggcgc ucgggggcgu ccuaaucuuc     2040

cuauccacag caguauccgc a                                               2061


<210>  2759
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) CDS opt

<400>  2759
acaagcguag gaaucgucgg gcuccugcua acgacagcaa uggcagcaga agucacucga       60

cgaggcuccg cauacuacau guaccuggac cgaaacgacg ccggagaagc cauauccuuc      120

ccaaccacac uuggcaugaa caaaugcuau auccaaauaa uggaccucgg acacaugugc      180

gaugccacaa ugucauacga augcccaaug cucgacgaag gcguggaacc agaugacguc      240

gacugcuggu gcaacaccac aagcaccugg gucguauacg guacaugcca ccacaagaaa      300

ggcgaagcac ggaggagccg acgagcagua acccuaccaa gccacuccac ucgcaaacuc      360

caaacccgaa gccaaaccug gcuagaaagc cgagaauaca ccaaacaccu aauacgggua      420

gaaaacugga ucuuccgaaa ucccggcuuc gcacuagccg ccgcggcaau cgcauggcuc      480

uuaggcucaa guacaagcca aaaaguaauc uaccucguca ugauucuacu aaucgccccc      540

gccuacucca uacgcugcau cggaguaagc aacagagacu ucgucgaagg gaugucaggc      600

gguaccuggg uggacgucgu acuagaacac ggcggcugcg uaaccgucau ggcccaagac      660

aaaccaacgg uagacaucga acucgucacc accacaguca gcaacauggc cgaagugcgc      720

uccuauugcu augaagcauc aauaucagac auggcuucag acucacgaug cccaacacaa      780

ggagaagccu accuagacaa acagucagac acccaauacg uaugcaaacg aaccuuaguc      840

gaucgcggau ggggaaacgg cugcggccuc uucgggaaag gcucgcuagu gacaugcgcc      900

aaauucgcau gcuccaagaa aaugaccggc aaaucaaucc aaccagagaa ccuagaguau      960

aggauaaugc ucucagucca cggaucgcaa cauagcggaa ugauagucca agacacggga     1020

cacgagacgg acgagaaccg cgcaaaaguc gaaauaacac caaacagccc ccgagcggaa     1080

gcgacacuag gcggguucgg aagccuagga cuagacugcg agccacgaac gggccuggac     1140

uucucagacc uauacuaccu caccaugaac aacaaacauu ggcuagucca caaagaaugg     1200

uuccacgaca ucccccuccc auggcacgcg ggugcagaca caggcacucc ccacuggaac     1260

aacaaagagg cccuggucga auuuaaagac gcucacgcga aaaggcaaac cgucguagua     1320

cugggcucac aagaaggcgc gguccauaca gcccucgcag gcgcauuaga agccgaaaug     1380

gacggagcaa aaggacgccu auccucaggc caccucaaau gccgacucaa aauggacaaa     1440

cuacggcuga aaggaguauc cuacagccuc ugcacagccg cauucaccuu caccaaaauc     1500

cccgccgaaa cccuacacgg gaccguaaca guagaaguac aauacgcagg gacagauggc     1560

ccaugcaaag ucccggccca gauggccguc gacaugcaaa cacuaacacc gguuggacgc     1620

cucaucacag ccaaccccgu cauuacagaa ucaacagaga acuccaaaau gaugcucgaa     1680

cuagacccac ccuucggaga uucauacauc gucauaggcg ucggagaaaa gaaaauaacu     1740

caccacuggc accgcagcgg cagcaccauc ggcaaagcau ucgaagccac aguccgagga     1800

gcaaaaagga uggcaguacu cggagacacc gcaugggacu uugguagcgu cggcggcgcc     1860

cuaaacucac uaggaaaagg aauacaucaa aucuucggag cagcauuuaa aagccucuuc     1920

gguggaauga gcugguucuc ccaaauacua aucggaaccc uccugaugug gcuaggacug     1980

aacaccaaaa acggaagcau cagccugaug ugccucgcgc ucgggggcgu acuaaucuuc     2040

uuaagcacag caguaucggc c                                               2061


<210>  2760
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2760
acaagcguug gaaucgucgg gcugcuacuc acgaccgcca uggccgcuga agucacgcgc       60

aggggcucag cauacuacau guaccucgac agaaacgacg cgggcgaagc aaucagcuuc      120

ccaacaacac ucggcaugaa caaaugcuac auacaaauaa uggaccucgg acacaugugc      180

gacgcaacaa ugucguacga augccccaug cucgacgaag gaguugaacc cgacgacgua      240

gacugcuggu gcaacacaac aaguaccugg gucguguacg gaaccugcca ccacaagaaa      300

ggagaagcua ggcggucacg acgcgcagua acccugccaa gccacuccac gagaaaacua      360

caaacccgca gccaaacaug gcuggaauca cgcgaauaca ccaaacaccu cauccgcgug      420

gaaaacugga uuuucagaaa cccaggcuuc gcgcuagccg ccgccgccau agcauggcua      480

cucgggucau ccacaagcca aaaagucaua uaccucguca ugauccuguu aaucgcccca      540

gcguacagca uacggugcau cggcgucagc aaucgagacu ucguggaagg cauguccggc      600

ggaaccuggg ucgacaucgu ccucgagcac ggagggugcg uaaccgucau ggcacaagac      660

aaaccaacag uagacauaga acucgugaca acaacaguau cuaauauggc cgaaguccgc      720

uccuacugcu acgaagccag caucucagac auggccuccg acucacgcug cccaacgcaa      780

ggggaagcau accucgacaa acaauccgac acccaauacg uaugcaaaag aacacucgua      840

gaccgagggu ggggaaacgg augcggccua uucggaaaag gcagccucgu aaccugcgcc      900

aaauucgcgu gcuccaagaa aaugacgggc aaaagcaucc aacccgaaaa ccucgaauac      960

agaaucaugc uguccgucca cggcucccaa cacuccggga ugaucguaaa cgacacggga     1020

cacgaaaccg acgaaaaccg agccaaagua gaaaucaccc caaacagucc cagagccgaa     1080

gccaccuugg gugguuucgg aagccucggc cucgacugcg aaccgagaac aggccuggac     1140

uucagcgacc ucuacuaccu cacaaugaac aacaaacacu gguuagucca uaaagaaugg     1200

uuccacgaca uaccucuccc cuggcacgcc ggcgcagaca ccggaacccc acacuggaac     1260

aacaaagagg cacuuguaga guucaaagac gcccacgcca aacgacaaac cgucguaguc     1320

cucggaucac aagaaggcgc gguacacacc gcacucgcgg gggcccugga agcagaaaug     1380

gacggcgcca agggcagacu cagcuccggg caccucaaau gcagacugaa aauggacaag     1440

cuccgacuca agggagucuc uuacucacua ugcaccgccg cguucacauu cacuaaaauc     1500

ccagccgaaa cacuccacgg uaccgucacc guagaagucc aguaugcagg cacugacggc     1560

ccgugcaaag ugcccgccca aauggccgua gacaugcaaa cacucacccc aguaggaagg     1620

cuaaucacug caaaccccgu gauaacagaa ucaaccgaaa acucaaaaau gaugcuagaa     1680

cuagacccac ccuucggaga cuccuacauc gucauaggag ugggggaaaa gaaaaucaca     1740

caccacuggc acagguccgg cuccacacuc ggcaaagccu ucuccaccac ccuaaaaggc     1800

gcacaacgcc uagcagcccu aggagacacg gcgugggacu ucggguccau aggcggcgua     1860

uucaacucca ucggacgcgc aguccaccaa gucuucggcg gagccuuccg aacccuauuc     1920

ggcggaauga gcuggauaac ccagggacug augggagcac uacugcuaug gaugggcguc     1980

aacgcacgcg acagaagcau agcacuagcc uuccucgcca cuggcggagu acucguauuc     2040

cuggcaacaa acguccacgc a                                               2061


<210>  2761
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2761
accagcgucg gaaucgucgg acuccugcua acaacagcaa uggcagccga agucacgcga       60

aggggaucgg cauacuacau guaucucgac cgcaacgacg cgggcgaagc caucagcuuc      120

ccaacaacgc ugggcaugaa caaaugcuac auacaaauua uggaccuagg acacacaugc      180

gacgcaacaa ugagcuacga augccccaug cucgacgagg gcguugaacc cgacgacguc      240

gacugcuggu guaauacgac auccacaugg guaguauacg ggaccugcca ccacaagaaa      300

ggggaagcgc gccgguccag gagggccguc acucucccga gccacaguac caggaaacua      360

caaacccgca gccaaaccug gcucgaaagc cgugaauaua caaaacaccu aauucgcgua      420

gagaacugga ucuuccgcaa ccccggauuc gcccuggcag cagcagcaau agccuggcug      480

uugggcucaa gcacaagcca aaaggucaua uaccugguaa ugauccuacu aauagcacca      540

gcauacucca ucagaugcau aggcgugagc aaccgagacu ucguugaagg aaugucgggc      600

gggacauggg ucgacguagu ccuagaacac ggaggcugcg uaacggucau ggcucaagac      660

aagcccacgg ucgacauaga gcuaguuacu acaacaguaa gcaacauggc cgaagucagg      720

agcuauugcu acgaagccuc caucucagac auggcauccg acucccggug ccccacccaa      780

ggagaagcau accucgacaa acaaagcgac acgcaauacg ucugcaaaag gacccucguc      840

gaccgcggau ggggcaacgg augcgggcua uucggaaaag gaucccucgu gaccugcgcc      900

aaauucgccu gcuccaagaa aaugacaggc aaaagcauac aacccgagaa ucuggaauac      960

agaauuaugc ucagcguaca cggcucacag cacagcggca ugaucguaaa cgacaccgga     1020

cacgaaacgg acgaaaaccg agcaaaaguc gaaauaacac caaacagccc ucgcgcggaa     1080

gcgacccugg gaggauucgg gagccuagga cuggacugcg aaccccgcac gggccuagac     1140

uucagcgauc ucuauuaccu aaccaugaac aacaaacacu ggcuagugca caaagagugg     1200

uuccacgaca ucccacuccc guggcacgcc ggagccgaca caggaacccc gcacuggaac     1260

aacaaagagg cccuuguaga guucaaagac gcacaugcga aaagacaaac agucguaguc     1320

cugggcucac aagaaggagc cguacacaca gcacuagcag gcgcucucga agccgaaaug     1380

gacggagcca aagggcgccu aagcuccggc caccugaaau gccgccuaaa aauggacaaa     1440

cuacgccuca agggaguauc auauucccua ugcaccgcag ccuucacauu cacaaaaauc     1500

ccagccgaaa cacuacacgg cacaguaaca gucgaaguac aauacgcagg aacagacggc     1560

ccaugcaaag uccccgccca aauggcgguu gacaugcaaa cccuaacccc agucggacgc     1620

cugaucacag caaacccagu caucaccgaa uccacagaga acucaaaaau gaugcucgaa     1680

cuagacccgc ccuucggcga uagcuacauc guaauagggg uaggagaaaa gaaaaucaca     1740

caucacuggc accgcagcgg aagcacccua ggcaaagcau ucucgaccac acugaaagga     1800

gcacaacggc ucgcagcccu cggagacacc gccugggacu ucggauccau agggggaguc     1860

uucaacucga ucggccgcgc aguacaucaa guauucggcg gcgcguucag gacacuauuc     1920

ggaggaauga gcuggaucac acaaggccua augggagccc uacuacucug gaugggcguc     1980

aacgcacgag accggagcau agcccuagca uuccucgcca ccggaggcgu acucguauuc     2040

cuagcaacga acguacacgc g                                               2061


<210>  2762
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       CDS opt

<400>  2762
accagcaucg gaauaaucgg acuccuccua acgaccgcaa uggcagcaga aauaacacga       60

cgaggcucag ccuacuacau guaccuggac cgcagugacg caggaaaagc gaucucguuc      120

gcaacuaccc ucggcgucaa caaaugccac guccaaauca uggaccucgg ucacaugugc      180

gacgcaacca uguccuacga augcccaaug uuagaugaag guguagaacc agacgacgua      240

gacugcuggu gcaacacgac gucaaccugg gucgucuacg guaccugcca ucacaagaaa      300

ggcgaagccc gccgcagcag acgcgccgua acccuaccaa gccacucaac acgaaaacuc      360

cagaccagau cacagacgug gcuggaaucc cgagaauaua ccaaacaccu aauaaaaguc      420

gaaaacugga uauucagaaa ucccggauuc gcccucgucg caguagcaau cgcuuggcua      480

cucggaucca gcacaagcca aaaaguaauc uaucucguaa ugauacuccu aauagcaccc      540

gcauacucca uaagaugcau cggcguaagc aaccgagacu ucgucgaagg aaugucgggc      600

ggcacauggg uagacguggu acuagagcac ggaggaugug uaacggucau ggcacaagau      660

aaaccgaccg ucgacauaga acucguaaca accaccguau ccaacauggc cgaagucaga      720

agcuacuguu acgaagccuc caucucagac auggccucag acucccgcug ccccacacag      780

ggcgaagcau accuagacaa gcaauccgac acacaauaug ucugcaaacg aacgcuaguc      840

gaccgaggau gggggaacgg augcggacuc uuugguaagg gcucacucgu aaccugcgcc      900

aaauuuaccu gcagcaagaa aaugaccgga aaaagcaucc aaccggaaaa ccuagaauac      960

agaauuaugc ucucggucca cggcucacaa cacagcggga ugauaggcua ugaaaccgac     1020

gaagacagag ccaaaguaga ggucacacca aacagcccca gggcagaagc aacccucggc     1080

ggcuucggau cccucggccu cgacugugaa ccccgaaccg gccuggacuu cuccgaccuc     1140

uacuaccuga ccaugaacaa caagcacugg uuaguucaca aagaaugguu ccaugacauc     1200

ccccuacccu ggcacgccgg cgcagacacc ggcaccccac acuggaacaa caaagaagcc     1260

cuagucgagu ucaaagacgc acacgccaaa cggcaaacag ucguaguccu uggcucccaa     1320

gaaggagccg uacacaccgc acucgccgga gcacuagaag cagaaaugga cggugccaag     1380

ggccgacucu ucagcggaca ccuaaaaugc cgccucaaga uggacaagcu aagacucaaa     1440

ggggucuccu acucucucug cacugcagcc uucaccuuca cgaaaguccc cgcagaaacc     1500

cuccacggga cugucaccgu agaaguacaa uacgccggua ccgacggccc augcaaaauc     1560

ccaguacaaa uggcaguaga caugcagaca cuuacacccg uaggacgccu aauaacagca     1620

aaccccguca uaaccgaaag cacagaaaac uccaaaauga ugcuagaacu agacccuccc     1680

uucggagaca gcuacauagu gaucggcguc ggcgacaaga aaaucacaca ccacuggcac     1740

agaagcggca gcacccuagg caaagcauuc ucuacaaccc ucaaaggcgc ccaacgacua     1800

gcagcacucg gagacacagc cugggacuuc ggaagcaucg gaggcgucuu caacagcaua     1860

ggacgcgccg uacaccaagu cuucggaggc gcuuuccgca cccucuucgg cggaaugagc     1920

uggaucaccc aagggcugau gggcgcacua cuacucugga ugggaguaaa cgcacgggac     1980

agaagcaucg cccuggcuuu ucucgccacc ggcggcgucc ucgucuuccu agcaacgaac     2040

guacaugcc                                                             2049


<210>  2763
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) CDS opt

<400>  2763
accuccgucg gcauaguagg ccuacuacuc accaccgcca uggccgccga agucacgcgc       60

agaggaagcg cauacuacau guaccuggac cgaaacgaug ccggcgaagc gaucuccuuc      120

ccaaccacuc ucgggaugaa caaaugcuau auccaaauca uggaccuagg acacaugugc      180

gaugcaacca uguccuacga gugcccaaug cuagaugaag gagucgaacc agacgacgug      240

gacugcuggu gcaacaccac cucgacaugg guagucuacg gcacaugcca ccauaagaag      300

ggggaagcca gacgcuccag acgagcagua acgcuccccu cacacucaac gagaaaacuc      360

caaaccagaa gccagacaug gcucgaauca cgcgaguaca ccaaacaccu cauccgcguc      420

gaaaacugga ucuuccgaaa uccuggcuuc gcccuagcag cagccgccau agccuggcua      480

cucggcagca gcaccagcca aaaaguaauc uaucuaguaa ugauccuacu aaucgcaccc      540

gccuacagca uacgcuguau cgguguauca aaccgcgacu uugucgaagg aaugucaggc      600

ggcacauggg ucgacguagu ccuagaacac ggcggcugcg ucacaguaau ggcacaagac      660

aaaccaacgg ucgacauaga gcuaguaaca acaacgguau ccaacauggc cgaaguccga      720

ucauacugcu acgaagccuc caucucagac auggcaucgg acucccgcug uccgacacaa      780

ggagaagcgu accuagacaa acaaucggac acccaauacg uaugcaaacg aacucuagua      840

gaccgcggcu ggggaaacgg auguggccuc uucgguaagg gcucacucgu gaccugcgca      900

aaauucgcau gcagcaagaa aaugaccggc aaauccaucc aaccugaaaa ccuagaauac      960

cgaauaaugc uaucagucca cgguucacaa cacuccggaa ugaucguaaa cgacaccggc     1020

cacgaaacag acgaaaaccg agccaaagua gaaauaaccc ccaacagccc acgagcggaa     1080

gcaacccuag ggggauucgg cucacugggg cuggacugug aacccagaac gggccuagac     1140

uucagcgacc ucuacuaccu aacaaugaac aauaaacacu ggcuaguaca caaagaaugg     1200

uuccacgaca ucccccuccc cuggcacgca ggcgccgaua ccggcacucc ccacuggaac     1260

aacaaagaag cucuggucga guucaaagau gcacacgcua aaagacaaac cguagucguc     1320

cuaggcuccc aggaaggugc gguccacacc gcccucgcag gagcccucga ggcagaaaug     1380

gacggcgcua aaggacgccu aagcuccggc caccucaaau gccgccuaaa aauggacaag     1440

uuacgccuca aaggagugag uuacagccuc ugcacagcgg ccuucaccuu cacaaaaauc     1500

ccagcagaaa cacuacacgg aaccgucacc gucgagguuc aauacgcagg cacggacggg     1560

ccaugcaaag ucccagccca aauggccguc gacaugcaaa cucuaacccc aguaggccgc     1620

cucauuacag ccaacccagu aaucacugaa uccaccgaga acagcaaaau gaugcucgaa     1680

cucgacccac cauucgggga cuccuacauc gucaucggcg uaggcgagaa gaaaauaaca     1740

caccacuggc accgcucggg gagcacucuc ggcaaggccu ucucaaccac acucaaaggc     1800

gcccaacgac ucgccgcccu aggagacacc gcaugggacu ucggcucaau cggcggaguc     1860

uucaacagca uaggccgcgc cguacaccaa guauucggcg gagcauuccg cacacuguuc     1920

gggggaaugu ccuggauaac acaaggccuc augggcgccc uauuacuuug gaugggggua     1980

aacgcccgag accgaagcau agcccuagcc uuccuagcca ccggaggggu acucguauuu     2040

uuagccacca acguccacgc a                                               2061


<210>  2764
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) CDS opt

<400>  2764
cgcaucauaa acgcccgcaa ggaaaagaaa agacgcgggg ccgacacauc cgucggaaua       60

guaggccugc uacuaacaac cgccauggcc gccgagguaa caagacgcgg cagcgccuau      120

uacauguacc ucgaccgcaa ugacgcagga gaagcaauau cauuccccac caccuuggga      180

augaacaaau guuacaucca aaucauggac cuaggacaca ugugcgacgc aacaaugagu      240

uacgagugcc caaugcugga cgaaggagug gaaccagacg acgucgacug uuggugcaac      300

accaccagca ccuggguagu auacggcaca ugccaccaca agaaaggcga agcacgacga      360

agccggagag cgguaacccu ccccagccac uccacacgca aacugcaaac cagaucccaa      420

accuggcuag aaagccgcga auacacaaaa caucucauac gcguggaaaa cuggauauuu      480

agaaacccag gcuucgcccu agccgccgcc gcaaucgcau ggcuccucgg aagcuccacc      540

agccaaaaag uuauuuaccu gguaaugaua cuguugauag cacccgccua cagcauucgg      600

uguaucggcg uaagcaacag agacuucguc gaaggaaugu cgggaggcac cugggucgac      660

aucguccuag agcacggcgg cugcguaacc gucauggcac aagacaaacc aacagucgac      720

aucgagcucg ucaccaccac cguaucaaac auggccgaag ugcgcagcua cugcuacgaa      780

gcaagcauau cagacauggc uucugacagc agaugcccca cacaaggaga ggccuaccua      840

gacaagcaau ccgacacaca guacguaugc aaacgaaccc uagucgaccg aggaugggga      900

aacggaugcg gauuauucgg caagggaucc cuagucacgu gcgcaaaauu cgccugcagc      960

aagaaaauga ccggaaaauc cauacaacca gaaaaccucg aauacaggau aaugcucagc     1020

guacacggcu cacaacacag uggcaugauc gucaacgaca ccggacauga aaccgacgaa     1080

aaccgcgcca aaguggaaau caccccaaac agcccacggg cagaggcaac acucggcggc     1140

uucggcuccc uaggacugga cugcgaaccc agaaccggac ucgacuucuc cgaccucuac     1200

uaccuaacaa ugaacaacaa acauuggcug gugcacaaag aaugguucca cgacauaccg     1260

cuucccuggc acgccggcgc cgacacggga acaccccacu ggaacaacaa agaagcccuc     1320

guagaguuca aagacgcaca cgcaaaacga caaacggucg ucguccuagg gucacaagaa     1380

ggcgccgucc acaccgcacu agccggcgca cucgaagcag aaauggacgg cgccaaagga     1440

cgacucucau caggccaccu aaaaugccgc cuaaaaaugg acaaacuacg ccugaaaggc     1500

gucagcuaca gccuauguac cgccgcauuu acguuuacca aaauacccgc agaaacacuc     1560

cacggcacag ucacagucga aguccaauac gccgggaccg acggccccug caaaguaccg     1620

gcccagaugg caguggacau gcaaacgcuc accccaguug gaagacugau aaccgcaaac     1680

cccgucauca ccgagagcac cgaaaacuca aaaaugaugc uagaacucga cccacccuuc     1740

ggcgacuccu acaucgucau cggcgucggu gaaaagaaaa uaacacauca uuggcacaga     1800

uccggaagca ccaucggaaa agcauucgaa gccaccgucc gaggagcaaa aagaauggcg     1860

guccucggcg acaccgcgug ggacuucgga agcgugggcg gcgcccucaa cagccuaggg     1920

aaagggauac accaaauuuu cggugcagcc uucaaaucac uauucggcgg aaugagcugg     1980

uucucccaaa uacuaaucgg uacacuacuc auguggcuag gccuaaacac caaaaacggc     2040

agcauaagcc uaaugugucu cgcucuaggc ggcguacuca ucuuccucuc uacagcaguc     2100

agcgccgacg uaggcugcag cgucgacuuc agcaaa                               2136


<210>  2765
<211>  2133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2765
aucaucaaug cucgaaaaga gaagaaacga cguggcgcag acacaucggu cggcauagua       60

ggacucuuac ucacaaccgc aauggcagcg gaagucaccc gacgcgggag cgccuacuac      120

auguaccucg accgcaacga ugccggugag gcaaucucau uccccacgac acuaggaaug      180

aacaaaugcu acauccaaau cauggaccuc gggcacaugu gcgaugcgac uauguccuau      240

gagugcccaa ugcuggauga aggagucgaa cccgacgacg uagacugcug gugcaacacg      300

accaguacau gggucguaua cggaacgugc caccacaaga aaggcgaagc ccgccgcagc      360

cgucgagcag ugacccuacc cagccacuca acacgcaaac uccaaacacg uucacaaacc      420

uggcucgaaa gccgagaaua cacgaaacac cuaauaagag ucgaaaacug gaucuuuagg      480

aacccgggcu ucgcacuagc agcggccgcg auagccuggc uccuggggag cucgaccuca      540

caaaaaguca uauaccuggu aaugauccua cugaucgccc cggcauacuc uauacgcugu      600

auaggcgucu cgaaccgcga cuucguagaa ggaaugagcg gagggacaug ggucgacaua      660

guccuagaac acgggggcug cguaacaguc auggcccaag acaaaccaac cgucgacauc      720

gaauuaguca caacuacagu cucgaacaug gccgaaguac ggagcuacug cuacgaagcc      780

agcaucagcg acauggccuc agacagccga ugcccaacac aaggcgaagc auaccucgac      840

aaacaaagcg acacacagua cguaugcaaa cgcacccuag uagacagagg auggggaaac      900

gguugcggac ucuucgggaa aggaagccua gucacaugcg caaaauucgc cugcagcaag      960

aaaaugacug gcaaaaguau acaaccagaa aaccucgaau accgaaucau gcucagcgug     1020

cacggaucac aacacucugg gaugauagua aacgacaccg gacaugaaac cgaugaaaac     1080

cgcgccaaag uagagaucac accaaacagc cccagggccg aagccacccu cggaggcuuc     1140

ggcagccugg gacuagacug cgaaccccgc acaggccucg acuucucaga ucuauacuac     1200

cucacaauga acaacaaaca cuggcuaguc cacaaagaau gguuccacga caucccucua     1260

ccguggcacg ccggcgcaga caccggcacc ccacacugga acaacaaaga agcccuagug     1320

gaguucaaag acgcacacgc aaagcgccag acaguaguag uacuagggag ccaagaagga     1380

gcaguccaca cagcacucgc cggggcccua gaagccgaaa uggacggcgc aaagggacgc     1440

cuguccucgg gacaccuaaa augccgacug aaaauggaca agcuccgacu aaaaggcgua     1500

agcuacaguc uaugcacagc cgcguucaca uucaccaaaa ucccugcaga gacgcugcac     1560

gggaccguaa caguagaagu acaauacgcc ggcacggacg ggcccugcaa aguaccagca     1620

caaauggccg ucgacaugca gacacucaca cccguaggca gacuaaucac agcaaacccc     1680

guaaucaccg agucgacaga aaacaguaag augaugcugg aacucgaccc acccuucgga     1740

gacuccuaca uaguaauagg cgugggugaa aagaaaauca cacaccacug gcaccgaagc     1800

ggaagcacaa uaggcaaagc cuucgaagca acaguaagag gggcaaaacg cauggccguc     1860

cucggcgaca cagcguggga cuucggaagc gucggaggcg cacucaacuc ccucggaaaa     1920

ggaauccacc aaauauucgg agcagccuuc aaaucccuau ucggcgggau gaguugguuc     1980

agccaaauac ugauaggcac ccugcuaaug uggcuaggac ucaacaccaa aaacggguca     2040

auaucacuca ugugccucgc gcuaggagga gucuuaaucu uccuaucaac agcugucagc     2100

gccgauguag gaugcuccgu ugacuuuuca aaa                                  2133


<210>  2766
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2766
aucaacgcaa ggaaggagaa gaaacgccga ggggccgaca cgagcguggg cauagucggc       60

cuccuacuca caaccgcgau ggcggcagag guaacaagaa gaggcagcgc cuacuacaug      120

uaccucgacc gcaacgacgc aggagaagca auaucauucc caaccacccu aggcaugaac      180

aaauguuaca uacaaaucau ggaccuaggc cacaugugcg acgccacaau guccuacgaa      240

ugcccaaugc uagacgaggg cguagagccc gacgacgucg acugcuggug caacaccacu      300

agcaccuggg ugguauaugg cacuugccau cacaagaaag gagaagcccg ccgcuccagg      360

agagcaguaa cccuccccag ccacagcaca agaaaacucc aaacaagauc ccaaacuugg      420

cuggaaucga gagaauacac caaacaucua auaagagucg agaacuggau cuuccgaaac      480

ccaggauucg cacuagccgc agcagcaaua gccuggcuuc uaggcucaag cacaucccag      540

aaaguaaucu accuagucau gauacucuug auagcaccag cauacucaau ccgcugcaua      600

ggaguaagca accgcgacuu cgucgaagga augagcggag gcacaugggu cgauaucgua      660

cucgagcacg gaggcugcgu uacggucaug gcacaagaca aacccaccgu agacaucgaa      720

cugguaacca caacagugag caacauggca gaaguacgca gcuacuguua cgaagcauca      780

aucucagaca uggcaucaga cagccgaugc ccaacacaag gcgaagcgua ccucgacaaa      840

caaagcgaca cacaauacgu auguaaacgg acccuggucg accgaggaug gggcaacgga      900

ugcggacugu ucggcaaagg aucgcucgua acaugcgcaa aauucgcaug cagcaagaaa      960

augaccggaa aaagcauaca acccgagaac cucgaauacc ggaucaugcu cucgguacac     1020

gggucccaac acagcggcau gauaguaaac gacacgggac acgaaacaga cgagaacaga     1080

gcgaaagucg aaauaacacc aaacagcccc cgagcagaag cgacccuggg cggcuucggg     1140

ucccugggac uagacugcga accaagaacu ggacuagacu uuuccgaccu auacuaccua     1200

accaugaaca acaaacacug gcugguacac aaagaauggu uccaugacau cccgcuccca     1260

uggcacgccg gggccgauac cggcacaccc cacuggaaca acaaagaagc ccuagucgaa     1320

uuuaaagacg cccaugccaa aagacaaaca gucgucguac ugggcagcca agagggcgcc     1380

guacacaccg cgcucgcagg cgcacuagaa gccgaaaugg acggagcaaa aggaagacua     1440

agcucaggcc accuaaaaug cagacucaaa auggacaaac ugcgccugaa aggagugucg     1500

uacucccuau gcacagcagc cuucaccuuc acaaaaaucc cagcagagac gcuacacggg     1560

acagucaccg uagaggugca auaugcaggg acagacggcc caugcaaagu acccgcccaa     1620

auggccguag acaugcaaac ccuaacacca gucggacgcc ucauaacagc aaacccagua     1680

auaacagaau cuacggagaa cucgaaaaug augcuggaac uagauccgcc cuucggcgac     1740

agcuacaucg uaauaggagu cggugaaaag aaaauaacac accacuggca cagauccggc     1800

agcacgauag gaaaagccuu cgaggcaaca guaaggggcg ccaaacgaau ggcaguacua     1860

ggcgacaccg ccugggacuu cgggucaguc ggaggggcac uaaacagucu cgggaaaggc     1920

auacaccaaa uuuucggagc cgccuucaaa agccuauucg gaggaauguc gugguucuca     1980

caaauccuua uaggcacgcu acuaaugugg cuugggcuaa auaccaaaaa cggcagcauc     2040

agccugaugu gccuagcacu cggaggcgug cucauauucc ucagcaccgc cgucagcgcg     2100

gacgucggau gcagcgugga cuucagcaag                                      2130


<210>  2767
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2767
aacgcgcgaa aagaaaagaa aaggcgaggg gccgacacaa gcguuggaau cgucggccua       60

cucuugacaa cggcaauggc agcagaagua acacggcgcg gaucagcgua cuacauguac      120

cucgacagaa acgacgccgg cgaagccauc agcuucccca cgacccucgg caugaacaaa      180

ugcuauauac aaaucaugga ccucggccac augugcgacg caacaaugag cuacgaaugu      240

ccaaugcucg acgaaggggu cgaaccagac gacguagacu guuggugcaa cacaacgagc      300

acauggguag uauacggaac augccaccac aagaaaggcg aagccagacg cagccgaagg      360

gcaguaacac uccccagcca cucaacacgc aaacuccaaa cccgaucaca aacguggcua      420

gaaucccgug aauacaccaa acaccuuaua cgcgucgaaa acuggaucuu ccgcaaucca      480

ggguucgcac ucgccgcagc agccaucgcg uggcugcuag gauccagcac aucgcaaaaa      540

guaaucuacc uaguaaugau ccuccuaauu gccccggcgu acucaauccg augcauagga      600

gucucaaacc gggacuucgu agaaggcaug agcggaggca cauggguaga cauuguccua      660

gaacacggcg ggugugucac cgucauggcc caagacaagc caaccgugga caucgaacuc      720

gucacaacaa ccguaucuaa cauggcagag guaagaucau acugcuacga agccaguaua      780

uccgacaugg caagcgauuc cagaugccca acccaaggcg aagccuaccu agauaaacaa      840

ucagacaccc aauacguaug caaacgcacc uuagucgacc gaggcugggg caauggaugc      900

ggccucuucg ggaaaggaag ccuagucacu ugugcgaaau ucgccugcag caagaagaug      960

acaggcaaau ccauccagcc cgaaaaccuc gaauaccgaa ucaugcuaag cguacacggc     1020

agccaacacu cuggaaugau agucaacgac acugggcacg aaacagacga aaaccgcgcg     1080

aaaguggaaa ucaccccaaa cucacccaga gccgaagcca cacuaggagg cuucgguuca     1140

cucggacucg acugcgaacc aagaaccggc cuugacuucu cagaccuaua uuaucuaaca     1200

augaacaaca agcacuggcu ggugcacaaa gaaugguucc acgacauacc ucucccgugg     1260

cacgcaggcg cagacacagg aacaccacac uggaacaaca aagaagcccu agucgaguuc     1320

aaagacgcac acgccaaacg acagacugua gucgugcuag gaagccaaga aggagccgug     1380

cacacugccc ucgcaggggc acuagaagca gaaauggacg gagccaaagg aagacuaagc     1440

agcggacacc ugaaaugcag acuaaaaaug gacaaacuaa ggcucaaagg agucaguuac     1500

ucacuaugca cagccgcguu cacauucaca aaaauacccg cagaaacgcu ccacggaaca     1560

gucacaguug aaguccaaua cgcaggcaca gacggccccu gcaaagugcc cgcccaaaug     1620

gcaguagaca ugcaaacacu cacgcccgua gggagacuaa uaacggcaaa cccggucaua     1680

accgaaagca ccgaaaacag caaaaugaug cucgaacucg auccgccguu cggcgauagc     1740

uacaucguca uaggagucgg cgaaaagaaa aucacacacc acuggcacag auccggaucc     1800

acaaucggaa aagccuucga agccacagug cgaggagcca aacguauggc gguacucggc     1860

gacacggccu gggacuucgg cagcgucggg ggagcacuga acagccuagg aaagggaauc     1920

caccaaaucu ucggagccgc cuuuaaaagu cucuucgggg gcaugucaug guucagucaa     1980

auacucaucg gaacacuacu cauguggcua ggccuaaaca ccaaaaacgg auccauauca     2040

cuaaugugcc uugcccuagg cggaguccua auauuccucu caacagcagu cagcgccgac     2100

gucgggugcu cggucgacuu cucaaaa                                         2127


<210>  2768
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2768
gccagaaaag agaaaaagag acgaggagcg gacacguccg ucggcaucgu cggucuccuu       60

cugacaaccg ccauggccgc ggagguaacc cgccgcggaa gcgccuacua uauguaccuc      120

gacagaaacg acgccggaga agcaaucuca uuccccacaa cccuaggaau gaacaaaugc      180

uacauccaaa ucauggaccu cggccacaug ugcgacgcca caaugagcua cgagugcccc      240

augcuagacg aaggaguaga gcccgacgac gucgacugcu ggugcaacac caccucaacc      300

uggguagucu acggaacaug ccaccacaag aaaggcgagg caagacgcuc ccgccgagcc      360

guuacgcuuc cgucacacag caccagaaaa cuccaaacca gaucccagac cuggcucgaa      420

uccagagaau acacuaaaca ccuuauacga gucgagaacu ggaucuucag gaacccaggc      480

uucgcguuag cagcagccgc gaucgccugg cuacucggca guuccaccag ccaaaaagua      540

auuuaccucg ugaugauccu acuaaucgca ccggcauauu cuauaagaug cauaggcguc      600

agcaacaggg acuucgugga aggaauguca ggaggcaccu gggucgauau cguacuagaa      660

cacggaggcu gcgucaccgu cauggcccaa gacaaaccaa cggucgacau cgaauuaguc      720

accacaaccg uaagcaacau ggcagaaguc agaagcuacu gcuacgaagc auccaucucc      780

gacauggcaa gcgacucacg cugccccacc caaggcgaag cauaccucga uaaacaaagc      840

gacacacaau acguaugcaa aaggacacuc guggacagag gaugggggaa cggcugcggc      900

cuauucggca aaggaagccu cgugaccugc gccaaauucg caugcuccaa gaaaaugacc      960

ggaaaaucaa uccaaccaga aaaucucgaa uacagaauca ugcucuccgu ccacgggucc     1020

caacacagcg gcaugauagu uaacgacaca ggccaugaga ccgacgaaaa ccgggcuaaa     1080

guagagauaa cccccaacuc accccgggcc gaagccacgc uagggggcuu cggaagccua     1140

ggacucgacu gugaaccgcg uacgggacuc gacuucuccg accuguauua ucucacaaug     1200

aacaacaaac acuggcuagu acacaaagag ugguuccacg acauaccgcu accauggcac     1260

gccggcgccg acaccggaac accacacugg aauaacaaag aagcccucgu agaguucaaa     1320

gacgcgcacg caaaaagaca aaccguggua gucuuaggcu cacaggaagg ggcaguacac     1380

acagcccugg caggagcacu agaagccgaa auggauggag caaaagggcg acuaagcagc     1440

gggcaccuua aaugccgacu caaaauggac aaacuacgcc ucaaaggagu cuccuacucg     1500

cuauguaccg ccgcauuuac auucaccaaa auccccgcag aaacccucca cggaacagua     1560

acaguagaag uccaauaugc cggcacagau ggcccaugca aaguaccugc gcaaauggca     1620

gucgacaugc aaacacuaac gccugucggc cgacuaauca cagccaaccc aguaaucaca     1680

gaaucgaccg aaaacagcaa gaugaugcua gaacuagacc cacccuucgg cgacuccuac     1740

auagucauag ggguaggaga aaagaaaauc acucaccacu ggcacaggag cggcuccacc     1800

auaggaaaag cguucgaagc uaccgucaga ggcgcaaaac gcauggcggu acugggagac     1860

accgcauggg acuucggaag cgucggaggg gcccuaaacu cccuaggaaa aggaauccac     1920

caaauauucg gagccgccuu caaaagccua uucggaggca ugagcugguu uucccaaaua     1980

cuaaucggca cccuccucau gugguuagga cuaaacacca aaaacggcag cauaucacuc     2040

augugccucg cgcuaggcgg cguccucaua uuccucagca cagcaguguc ggccgacguc     2100

ggcugcagcg uagacuuuuc aaaa                                            2124


<210>  2769
<211>  2121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2769
agaaaagaga agaaacgaag aggagcagac accagcguag gaauagucgg acuccuccua       60

acuaccgcaa uggcagcaga agucacgcga agaggcagcg cguacuacau guaccuagac      120

cggaacgaug ccggcgaggc caucucauuc ccaacaacac ucgggaugaa caaaugcuac      180

auacagauca uggaccucgg gcacaugugc gacgccacua uguccuacga gugcccgaug      240

cuggacgaag gcgucgagcc agacgacguc gacuguuggu guaacacuac cagcaccugg      300

guaguauacg gaacaugcca ccacaagaaa ggagaagccc gcaggagcag acgcgcgguc      360

acgcugccau cccacagcac gcgcaaacuc caaacuaggu cacaaaccug gcuagagagc      420

cgagaauaca ccaagcaccu aaucagaguc gaaaacugga uauuccgcaa cccaggauuu      480

gcacuagcgg ccgccgcaau agcguggcua cuaggcucga gcacauccca aaaagugauc      540

uaccuaguca ugauacuccu aaucgcaccc gcauacagca uccgaugcau aggagucucu      600

aacagagauu ucgucgaagg aaugucgggc ggaacguggg ucgacauagu acuagaacac      660

ggaggcugcg uaaccgugau ggcccaagac aaaccaaccg ucgacaucga gcuagucacg      720

acuaccguaa gcaacauggc agaaguacga uccuacugcu acgaagcaag caucucagac      780

auggccagcg acagccgaug ccccacccag ggcgaagcau accuagacaa acaaucagac      840

acacaauacg ucugcaaaag aacacucgua gaucgcggau ggggcaacgg augcggacuc      900

uucggaaaag gcagucuagu cacaugcgcc aaauucgcau gcucaaagaa aaugaccggu      960

aaauccauac aaccagaaaa ccucgaauac cggauaaugc ucucaguaca ugggagccaa     1020

cacagcggca ugauagucaa cgacacaggg cacgaaacug acgaaaaccg cgccaaagua     1080

gaaauaacac caaacagccc ccgcgccgaa gccacacugg gaggcuuugg aagccucggc     1140

uuagauugcg agccacgaac aggccuagac uucucggacc uauacuaccu cacgaugaac     1200

aacaaacacu ggcuaguaca uaaagaaugg uuccaugaca ucccccuccc auggcacgcu     1260

ggcgcggaca cggggacacc acacuggaac aacaaggaag cccugguaga guucaaggac     1320

gcccacgcga aaaggcaaac agucguggua uuaggcagcc aggaaggcgc cguccacacc     1380

gcauuagccg gcgcccugga agccgaaaug gacggagcaa aaggcagauu aagcuccgga     1440

caccucaaau gccggcuaaa gauggacaaa cucagauuga aaggaguauc cuacagccuu     1500

ugcaccgcag ccuucaccuu cacgaaaaua ccagccgaaa cacuacacgg aacaguaacg     1560

gucgaagugc aauacgcagg aacagacggc cccugcaaag uaccagcgca aauggcaguc     1620

gacaugcaaa cccugacgcc ggucggcaga cuaaucaccg caaacccagu caucacagaa     1680

uccaccgaaa acucaaaaau gaugcuagaa cuggacccac ccuucggaga cuccuacauu     1740

guaauaggag ucggcgaaaa gaaaauaacc caccacuggc accgaucagg cagcacaaua     1800

ggcaaagcau ucgaagccac cguaagaggc gcaaaacgca uggcaguacu gggagacacc     1860

gcaugggacu ucggcagcgu uggcggagcc cucaacuccu uaggcaaagg aauccaccaa     1920

auauucggag cagcauucaa aagccuguuu ggcgguauga gcugguucuc ccaaauacuc     1980

aucgggacac ugcugaugug gcucggacuc aacacgaaaa acgguagcau aagucugaug     2040

ugccucgccc uaggcggcgu acucaucuuc cuaaguacag cgguaucagc agacguaggc     2100

ugcagcgucg acuucagcaa a                                               2121


<210>  2770
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2770
aaagaaaaga aacgccgagg cgcagacacc aguguaggca uaguaggacu ucuccuaaca       60

acagccaugg cggcggaagu aacucgcaga ggcuccgccu acuacaugua ucucgaccga      120

aacgacgcgg gugaagcaau cagcuuccca accacucucg gcaugaacaa augcuacauc      180

caaaucaugg accucggcca caugugcgac gccacaauga gcuaugaaug ccccaugcua      240

gacgaaggcg uagaacccga cgacguagac ugcuggugua acaccaccuc aaccuggguc      300

gucuacggaa cgugccacca uaagaaagga gaagcacgcc gcagcagacg cgcggucaca      360

uuaccaagcc auagcacgcg caaacuacaa acccggucac agacauggcu cgagagccga      420

gaguacacca aacaccuaau acgcgucgaa aacuggauau uccgaaaccc cggauucgca      480

cuugcagcgg ccgccaucgc auggcuccug ggcucaagca ccagccaaaa aguaaucuac      540

cuagucauga uccugcuaau cgccccagcc uacagcauca ggugcauagg cgugagcaac      600

cgagacuucg ucgaaggaau gucgggcgga accuggguag acaucguacu ggaacauggc      660

ggcugcguca ccguaauggc ccaagacaag cccaccgucg acauagaacu cgucacaacu      720

accgucucca acauggccga gguacgcucc uacugcuacg aagccucaau cuccgacaug      780

gccagcgacu cacgaugccc uacgcaaggc gaagccuacc uggacaaaca aucggacaca      840

caauacgugu gcaaacgaac auuagucgac cgaggauggg ggaacggcug cggccuguuc      900

ggcaaaggcu cauuagugac cugcgcaaag uuugccugca gcaaaaagau gaccggaaaa      960

uccauacaac cagaaaaccu agaauacaga aucaugcugu caguacaugg cucacaacac     1020

agugguauga ucgucaacga cacgggccac gaaacagacg aaaaccgagc gaaaguagaa     1080

aucaccccaa acagcccgcg cgcugaagcc acacucggag guuucggcuc acuaggccua     1140

gacugcgagc cacgaacugg ccuugacuuc uccgaccugu acuaccucac caugaauaac     1200

aaacacuggc uaguacacaa agaaugguuc cacgacaucc cacuacccug gcacgccggc     1260

gccgacaccg ggaccccaca cuggaacaac aaagaagcac ugguagaguu caaagacgcc     1320

cacgccaaaa gacaaaccgu cguaguacua ggcagccaag aaggugcggu ccacaccgcc     1380

cuagccggcg cccuagaagc cgaaauggac ggugccaaag gccgccuuag cuccggucac     1440

cuaaaaugcc gacuaaaaau ggacaaacug agacuaaaag gcgucucaua cucauuaugc     1500

acagcagccu uuacuuucac aaagauccca gccgagacgc uacacggaac aguaacagua     1560

gaaguacagu acgccgggac cgacggcccc ugcaaagucc ccgcccaaau ggccguagac     1620

augcagacac uaaccccagu cgggagacug aucaccgcca acccgguaau aacagaaagc     1680

accgaaaacu ccaaaaugau gcuugagcua gacccaccuu ucggggauag cuacaucguc     1740

aucgggguag gcgagaagaa aauaacacau cacuggcaua gaagcggcag cacgauagga     1800

aaagcauucg aagcaacagu ccgcggcgca aaacgcaugg ccguuuuggg cgacacagca     1860

ugggacuucg gcuccguggg cggcgcacuc aacuccuuag gcaaaggcau ccaccagauc     1920

uucggggccg ccuucaaauc acuauucggc ggcaugucau gguucagcca gauccucaua     1980

ggcacacucc uaauguggcu gggacuaaau accaaaaacg gcuccaucuc acucaugugc     2040

cuugcccucg gaggcguccu aaucuuccuu aguacagccg uaagcgccga cgucggaugc     2100

uccguagacu ucuccaaa                                                   2118


<210>  2771
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2771
gaaaagaaaa gacgcggcgc cgacacgagc gucgguauag uaggacuucu acucacgacc       60

gccauggcgg ccgaaguaac cagacggggc agcgccuacu acauguaucu cgaccgcaac      120

gacgccggcg aagccaucuc guuccccacc acacuaggga ugaacaaaug uuacauccaa      180

auaauggacc ucgggcacau gugcgacgcg acaaugaguu acgaaugccc aauguuagac      240

gaaggcguag aaccggacga cgucgauugc ugguguaaca ccacaucaac cuggguaguc      300

uacgggaccu gccaccacaa aaagggcgaa gcccgaagau cacggagagc cguuacccua      360

ccgagccacu ccacaagaaa auuacaaaca cgaagccaga ccugguuaga aucaagagag      420

uacacaaaac accugauaag aguagagaac uggaucuucc gaaacccggg cuucgcacua      480

gccgccgccg caaucgccug gcuccuagga uccucaacca gccaaaaagu cauuuaccuc      540

guaaugaucc uccucauagc accagcauac uccauccgau gcaucggugu uuccaacaga      600

gacuucguag aaggcauguc gggcggcacc ugggucgaca ucguccuaga gcacggaggc      660

ugcgugacug ucauggcaca agacaagccc accgucgaca ucgagcuagu aaccacaaca      720

guauccaaca uggcugaagu ccgaagcuac ugcuacgaag caucaaucuc ugacauggcc      780

agcgacagcc gaugccccac acaaggagaa gccuaccuag acaaacaaag cgacacccaa      840

uacgucugca agcgcacccu agucgaccgc ggauggggaa acgggugcgg acucuucggg      900

aagggaagcc uaguaacaug cgccaaauuc gcgugcucaa agaaaaugac gggcaagagu      960

auccagcccg aaaacuuaga auacagaauc augcucucgg uucacggauc ccaacacagc     1020

ggcaugauag uaaacgacac cggccacgaa acagacgaaa acagggcaaa aguagaaauc     1080

acccccaacu cacccagagc cgaagccacg cucggcggcu ucgggucacu cgggcucgac     1140

ugcgagcccc gaaccggacu ugacuucagc gaccuuuacu accuaacaau gaacaacaag     1200

cacugguuag ugcacaaaga gugguuccac gauaucccac uccccuggca cgcaggcgca     1260

gacaccggua caccccacug gaacaacaaa gaagcacucg uagaguucaa agaugcccac     1320

gcaaaaaggc aaaccgucgu aguauuaggc agccaagaag gcgcaguaca caccgcccuc     1380

gccggagccc uagaagcaga aauggacggc gcaaaaggaa gacucuccuc aggccaccuc     1440

aaaugccgau ugaagaugga caaacuucga cucaagggag ucaguuacuc auuaugcacu     1500

gcagccuuca ccuucaccaa aaucccggca gaaacgcuac acggaaccgu aaccgucgaa     1560

guccaauaug caggcaccga cggcccgugc aaaguacccg cccagauggc ggucgacaug     1620

caaacgcuga caccgguagg ucgccucaua accgccaacc ccguuauaac ggaauccacc     1680

gaaaacucca aaaugaugcu cgaacuagac ccgcccuucg gagacagcua caucgugauc     1740

ggugucggag aaaagaaaau aacccaccac uggcacagaa gugggagcac aaucggaaaa     1800

gccuucgaag ccaccgucag aggcgccaaa cgcauggccg uccucggcga cacagcaugg     1860

gacuucggga gcgucggcgg ugcccuaaac agccucggca aaggcauaca ccaaauauuc     1920

ggagcagcuu ucaagagcuu auucgggggc augagcuggu ucucccaaau ccuaaucggg     1980

acccuacuca uguggcucgg acuaaacacc aaaaacggaa gcauuagccu caugugccug     2040

gcccucgggg gcguacucau cuuccugucg accgccguaa gcgcugaugu cggaugcucc     2100

guagacuucu caaaa                                                      2115


<210>  2772
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2772
aagaaacgac ggggcgcaga cacauccguc ggcaucguag gacuccugcu aaccacagca       60

auggcagccg aagucacacg caggggcucu gcauacuaua uguaccuaga ccggaacgac      120

gcaggagaag ccauaucguu cccaaccaca cugggaauga acaaaugcua uauacagauu      180

auggaccucg gccacaugug cgacgccacc augagcuacg aaugcccuau gcucgacgag      240

ggcguggaac cagacgacgu cgacugcugg ugcaacacga caagcaccug gguggucuac      300

ggaaccugcc accacaagaa aggcgaggcg agacgcuccc gacgcgcggu cacccuccca      360

agccacucaa cgcgcaaacu acagacaaga ucccaaacau ggcucgaaag ccgcgaauac      420

accaaacacc uaauccgagu cgaaaacugg aucuuccgca accccggauu cgcacuagcc      480

gccgccgcaa uagcauggcu cuuaggcuca agcaccagcc aaaaaguaau cuaccucgua      540

augauccuuc ucaucgcacc agccuauuca auaagaugca uaggaguuag caacagagac      600

uucguagaag gaaugucggg aggaaccugg gucgacaucg uccucgagca cgggggcugc      660

gucaccguca uggcucagga caaaccaaca gucgauaucg agcuagucac cacgacagua      720

agcaacaugg cagaaguacg guccuacugc uacgaagcau cgaucuccga cauggcauca      780

gacucacgau gcccaaccca aggcgaagca uaccuagaca agcaaucaga cacgcaguac      840

guaugcaagc gaacacuggu agacagaggc uggggcaacg gaugcggccu auucggaaag      900

ggcucacugg uaaccugugc aaaauucgca ugcucaaaga aaaugacagg aaaaagcaua      960

caacccgaaa accucgagua ccguauaaug cucucagucc acggaagcca acauuccggc     1020

augaucguaa acgacacagg acacgagacc gacgaaaaca gagccaaagu cgaaaucacg     1080

cccaauagcc cacgcgccga agccacccua ggaggcuucg gaucacuagg auuggacugc     1140

gaaccacgua ccggccuaga cuucucagac cuauacuacc uaaccaugaa uaacaaacac     1200

ugguuagugc acaaagagug guuccacgac auaccacucc cauggcacgc gggcgcggac     1260

acaggcacac cccacuggaa caacaaagaa gcccuaguag aguucaaaga cgcacacgca     1320

aaacggcaaa ccguaguagu ccugggcagc caagaaggcg ccguacacac cgcgcuggcc     1380

ggagcccucg aagcagaaau ggacggcgcg aaaggaagac uauccuccgg ccaccuaaaa     1440

ugccgacuca aaauggacaa gcuaaggcug aaaggcguca gcuacucccu cuguaccgca     1500

gcguuuaccu ucaccaaaau cccagcagaa acccuccacg gaacagugac aguagaaguc     1560

caauacgccg gcacagaugg cccuugcaag guaccagcgc aaauggcggu cgacaugcaa     1620

acacuaacac cggucggccg ccucauaaca gccaaucccg uaaucacaga aagcaccgaa     1680

aacagcaaaa ugaugcucga acuagacccg ccauucggcg acagcuacau cgugauagga     1740

gucggcgaaa agaaaauaac acaucauugg cacagaagcg gcuccacaau cggcaaagca     1800

uucgaagcaa caguaagagg cgccaaacga auggcagucc ugggugacac agcgugggau     1860

uucgggucag ucggaggugc ccuuaacagc cuaggaaaag ggauccacca aauauucggg     1920

gccgcauuca aaucacuuuu cgggggaaug uccugguucu cccaaauccu caucggaaca     1980

cuccuaaugu ggcucggacu caacaccaaa aacggcagca ucagccucau gugccucgca     2040

cucggaggcg uacuaauauu ccugucaacg gcaguuagcg cagacguagg gugcucaguc     2100

gacuucagca aa                                                         2112


<210>  2773
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2773
aagaggagag gggcggacac cagcgucggc auagucggcc uacuacucac gaccgcaaug       60

gccgccgaag uaacacguag aggaucggcc uacuacaugu accucgacag gaacgacgcc      120

ggagaagcca ucucauuccc cacaacacug ggaaugaaca aauguuacau ccaaaucaug      180

gacuuaggac acaugugcga cgccaccaug ucauacgaau gccccaugcu cgacgaaggc      240

guggaacccg acgacgucga cugcuggugu aacacaacau caaccugggu agucuacggc      300

acaugccacc acaagaaagg cgaagcccgu cgcagcagaa gggcaguaac ccucccaagc      360

cacucaacgc ggaaacugca aacccgaagu caaaccuggc uggaaucgag ggaauacaca      420

aaacaccuaa uccgaguaga aaacuggauc uuccgcaauc caggcuucgc acucgccgca      480

gcagccauag ccuggcuacu cggcagcucc acaagccaaa aaguaauuua ccucgucaug      540

auacuacuga ucgcccccgc cuacuccauc agaugcaucg gcgucucaaa ccgcgauuuc      600

gucgaaggca ugucaggugg caccugggua gacaucgucc ucgaacacgg aggcugcguc      660

accgucaugg cacaagacaa acccacaguc gacaucgaau uggucacaac caccgucagc      720

aacauggccg aaguacgaag cuacugcuac gaagcgucaa uauccgacau ggcgagcgac      780

ucccgauguc caacucaagg agaagcguau cucgacaagc aaucggacac ccaauacgua      840

uguaaacgaa cccuaguaga ccgcggaugg ggcaacggau gcggccuauu cggaaaaggc      900

ucccucguga caugugcaaa guuugccugc agcaagaaaa ugacaggcaa aagcauccaa      960

ccagaaaacc uagaauaccg aaucauguug ucaguacacg gcucccaaca caguggaaug     1020

auagugaacg acacaggcca cgaaacagac gagaaucgcg ccaaagucga gauaaccccu     1080

aacucaccac gcgcagaagc gacacuggga ggauucgggu cccuaggccu cgacugcgaa     1140

ccacgcaccg gcuuagacuu cagcgaucuc uacuaccuaa ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgauauc ccacuccccu ggcacgccgg cgcugacaca     1260

ggcacgcccc acuggaacaa caaagaagcg cuuguggagu ucaaggacgc ccacgcaaaa     1320

aggcaaaccg ucguaguacu aggcucacaa gagggcgccg uacacaccgc ccuggcaggc     1380

gcacucgaag ccgaaaugga cggcgccaaa ggacggcucu ccagcggaca ccucaagugc     1440

cgccuaaaaa uggacaaacu gagacuaaaa ggcguaucau acagccugug caccgccgca     1500

uucaccuuca ccaaaauacc cgcggaaacc cuacacggca caguaaccgu agaaguacaa     1560

uacgcaggca ccgacggccc cugcaaagua ccggcccaga uggccguaga caugcaaacc     1620

cucacucccg ucggacgccu caucacagca aaccccguca uaacagaauc caccgaaaac     1680

agcaaaauga ugcucgaacu cgacccaccc uucggagacu ccuacauagu aaucggaguc     1740

ggcgagaaga aaaucaccca ccacuggcau cgaucaggau caacgaucgg caaagcauuc     1800

gaagcaaccg ugcggggagc aaaaaggaug gccguacucg gcgacaccgc cugggacuuc     1860

gggucagucg ggggcgcccu aaacucccug ggaaaaggca uucaccaaau cuucggugcc     1920

gccuucaaaa gccuguucgg gggcaugagc ugguucagcc aaauacucau aggcacacuc     1980

cugauguggc uaggccucaa caccaagaac ggcagcauca guuuaaugug ccuagcccug     2040

ggaggagucc ucaucuuccu cagcacagcc guaucagcag acguaggcug uagcgucgac     2100

uucagcaaa                                                             2109


<210>  2774
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2774
cgccggggcg cagacacauc agugggcauc guaggccucc ugcucaccac cgcgauggcc       60

gcagagguca cccgacgcgg gagcgccuac uacauguacc uagaccgaaa cgaugccggc      120

gaagccaucu cguucccgac aacccugggc augaauaaau gcuacaucca aauaauggac      180

cuaggucaca ugugcgacgc aacuaugucu uacgaaugcc cgaugcucga cgagggugug      240

gaaccugaug acguagacug cuggugcaac accacaagca cauggguggu auacggaaca      300

ugccaccaca agaaaggcga agcacgacgg agccggcgcg cagucacccu accgagccac      360

ucaacccgaa aacuccaaac ccgcagccaa acaugguugg aaagccgcga auacacaaaa      420

caccuaaucc gcgucgaaaa cuggaucuuc cgcaacccgg gcuucgcccu cgcagccgca      480

gccaucgccu ggcuuuuagg cucauccacc ucacaaaaag uaaucuaccu agucaugauc      540

cuacucauag ccccugcaua cuccaucagg ugcauaggcg uauccaacag ggacuucguc      600

gaaggcauga gcggcggcac cugggucgac aucguacucg aacacggggg cugcguaaca      660

gucauggcac aagacaaacc cacagucgac aucgaacucg ugacaaccac agucagcaac      720

auggcagaag uaagaucaua cugcuacgaa gcauccauaa gcgacauggc aucugacagc      780

agaugcccaa cacagggaga agcauaccuc gacaaacaaa gcgacacaca auacgucugu      840

aaacgaaccc uaguagaccg cggaugggga aacggcugcg gacucuucgg aaaaggcagc      900

cucguaacau gcgccaaauu cgccugcagc aagaaaauga ccggcaaauc cauacaaccc      960

gaaaaccucg aguacagaau caugcuuuca guccacggaa gccaacauuc cggcaugaua     1020

gucaacgaca ccggacacga aaccgacgaa aacagagcga aagucgaaau cacacccaac     1080

ucacccaggg cggaagcaac acugggagga uucgggucac ucggucuuga uugugaaccc     1140

cgaacuggac ucgacuucuc cgaccucuac uaccucacca ugaacaacaa acacuggcua     1200

guccacaaag agugguucca cgacauccca cuacccuggc acgcaggagc cgacacaggc     1260

accccacacu ggaacaacaa agaagcacug gucgaguuca aagacgcaca cgccaaacgc     1320

caaacugucg ucguacuagg cagccaggaa ggagcagucc acacagcacu ggccggagcu     1380

cuagaagcag agauggacgg cgcgaaagga cgccuaagcu caggccacuu aaaaugccga     1440

cucaaaaugg acaaacuccg ucuuaaagga gucucguacu cacugugcac cgcugcauuc     1500

accuuuacca agauccccgc ggaaacccua cacggaaccg ucacaguaga aguccaauac     1560

gccggaacug acggcccaug caaagucccc gcccaaaugg caguggauau gcaaacccua     1620

acccccguag gacgccuaau aacagccaac cccguuauca ccgaauccac cgaaaacucu     1680

aaaaugaugc uggaacuaga uccacccuuc ggagacuccu auauaguaau cggcgucgga     1740

gaaaagaaaa ucacgcacca cuggcaccgg agcggcucaa ccaucggaaa agccuuugag     1800

gcgaccgucc gcggagccaa acgcauggca guacucggag acacagccug ggacuucggg     1860

agcgucggag gcgcccuaaa cucacucggc aaaggcaucc accaaaucuu cggagccgca     1920

uucaaauccc ucuucggcgg caugagcugg uucucacaga uacuaauagg aacccuccua     1980

auguggcucg gccucaacac caaaaacggu agcaucagcc uaaugugccu agcacuaggc     2040

ggcguacuaa uauuccucuc caccgccguc agcgccgacg ugggcuguuc cgucgacuuc     2100

uccaaa                                                                2106


<210>  2775
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2775
agaggcgccg auacuagcgu aggaaucgug ggacuccuac ucacaacugc aauggccgca       60

gaagucacaa gacgaggaag cgcauacuac auguaucuag accggaacga cgccggagaa      120

gcaaucagcu ucccgacaac acuaggaaug aauaaaugcu auauccaaau aauggaccua      180

ggccacaugu gcgacgcgac caugagcuac gagugcccca ugcucgauga aggcgucgaa      240

ccagacgacg ucgacugcug gugcaacacu acaagcacau ggguaguaua cgggacaugu      300

caccacaaga aaggagaagc cagacgcucg cgccgcgcag ucacacuccc cucacacuca      360

acacggaaac uacaaacgcg cucgcaaacc ugguuggaau cccgagaaua caccaaacac      420

cuaauccgcg ucgaaaacug gaucuuccgc aacccggguu ucgcacucgc agccgcagcc      480

aucgccuggc uacucggcag cucaacaagc caaaaaguca uauaccuagu aaugauacua      540

cugaucgcac cagccuacag caucagaugc aucggcgucu caaaccgcga cuucgucgaa      600

ggaauguccg gcggaaccug gguagacauc guacuagaac acggcggaug cgucaccguc      660

auggcacaag acaaacccac aguagacauu gagcucguaa caacaacagu cuccaacaug      720

gccgaggucc gaucauacug cuacgaagca uccaucagcg acauggccag cgacuccagg      780

ugcccgaccc aaggcgaagc cuaucucgac aaacagagcg acacgcagua cgucugcaaa      840

cgaacauuag ucgaccgagg cuggggaaac ggaugcggcc uauucggaaa aggaagccua      900

gucacgugcg caaaauucgc augcucaaag aaaaugaccg gaaaaucgau ccaacccgaa      960

aaccuggaau accgaauaau gcucuccgua cacgggagcc agcacucagg aaugauaguc     1020

aaugacaccg gccacgaaac agacgagaau cgagcuaaag uagagauaac acccaacagc     1080

cccagagcag aagcgacccu cggcggcuuc ggcucccucg gcuuagacug cgaaccacgc     1140

accggccucg acuucagcga ccuauacuau uuaacaauga acaacaaaca cuggcuagug     1200

cacaaagaau gguuccacga cauaccacua ccauggcacg caggggcaga cacggggacc     1260

ccacacugga acaacaaaga agcccuagua gaguucaaag acgcacacgc aaaacgacaa     1320

acaguagucg uacuugggag ccaggaaggc gcaguccaca ccgcacuagc cggggcccuc     1380

gaagcagaaa uggacggggc aaaaggcaga cuaagcagcg gacaucugaa augcagacuc     1440

aaaauggaca aacuccgccu aaaaggcgug uccuacucac uaugcaccgc agccuucacc     1500

uucacgaaaa ucccagcgga aaccuuacac ggcaccguca caguggaagu acaauacgcc     1560

ggcaccgacg gaccaugcaa aguaccagca cagauggccg ucgauaugca aacacugacg     1620

cccgucggac gacugaucac ugcaaacccg guaaucaccg aaagcacaga aaacucaaaa     1680

augaugcucg agcucgaccc ucccuucgga gacagcuaca ucgucaucgg agucggagaa     1740

aagaaaauaa cucaucacug gcaccgcagc ggaucaacca ucggcaaagc cuucgaagcu     1800

acagucagag gcgcaaagag aauggcggua cuaggcgaca cagcauggga cuucggauca     1860

gugggaggcg cacugaacag ccucggaaag gggauacacc aaaucuucgg cgccgcauuc     1920

aaaagccucu ucggcggcau gagcugguuu ucccagauac uaaucggaac gcuccuaaug     1980

uggcuagggc uaaacaccaa aaacggcagc auaagccuga ugugccucgc gcucggaggc     2040

guccucaucu uccuaucaac cgcagucucg gcagauguag gcugcucagu cgacuucagc     2100

aaa                                                                   2103


<210>  2776
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2776
ggggccgaca cuuccguagg caucgucgga cuacuccuga ccacggccau ggccgccgaa       60

guaacccgcc gcggcagcgc auacuacaug uaccucgacc gcaacgacgc cggggaagcu      120

auaucguucc caacaacccu cggaaugaac aaaugcuaca uacaaaucau ggaccucggc      180

cacaugugcg acgcaaccau gagcuacgaa uguccaaugc uggacgaagg gguagaacca      240

gacgacgugg acugcuggug caacaccaca agcaccuggg ucgucuacgg aaccugccac      300

cacaagaaag gagaggcccg acgaagucgc cgcgcaguca cccucccaag ccacucuaca      360

cgaaaacuuc aaacccgguc acaaacaugg cucgaaucaa gagaauacac caaacaccuc      420

auccgagucg aaaacuggau cuucagaaac cccggauucg cccucgcagc ggccgccaua      480

gcguggcuac uaggcucaag caccucacaa aaagucaucu accuggucau gauccuccua      540

auagcacccg ccuacagcau ccgcugcauc ggcgucucaa acagagacuu cguagaagga      600

augagcggag gaacaugggu agacauagug cucgaacaug gaggcugcgu aaccgucaug      660

gcccaagaca aaccaaccgu cgacauagaa cuggucacua caaccguauc uaacauggcc      720

gagguaagau cauacugcua cgaagccucc aucuccgaca uggccaguga cagccgaugc      780

ccaacgcaag gcgaagccua ucucgacaaa caauccgaca cccaauaugu cugcaagcgc      840

acacucguag accgcggcug gggaaacggc ugcggccucu ucggcaaagg cagccuagua      900

accugcgcaa aauucgcaug cuccaagaaa augacaggaa aauccaucca acccgaaaac      960

cucgaauacc gcaucaugcu cuccgugcac ggcagccaac acuccggaau gaucguaaac     1020

gacaccggac acgaaacaga cgaaaaccgc gccaaagucg aaauaacacc aaacucccca     1080

cgcgcggaag ccacacuagg cggcuucggc ucccuaggcc ucgacugcga acccagaaca     1140

ggacucgacu ucagcgaccu cuauuaccua accaugaaca acaaacauug guuaguacac     1200

aaggaauggu uucacgacau cccccucccc uggcacgcag gcgcagauac aggaacacca     1260

cacuggaaca acaaagaagc ccucguagaa uuuaaagacg cccacgcuaa acggcaaaca     1320

guaguagucc uaggcagcca agagggggcc guacacaccg cacucgcagg cgcccucgaa     1380

gcugaaaugg auggcgccaa aggcaggcuc ucaucggguc accucaaaug ccgacuaaaa     1440

auggacaaac uacgucuaaa aggggucucu uacucacucu guacggcggc cuucaccuuc     1500

accaaaauac cagcagagac acuacacgga accgucacag ucgaggucca auacgccggc     1560

acagacggac caugcaaagu cccugcacaa auggccgucg acaugcaaac ccucacacca     1620

guaggacgcc ucaucacggc caaccccguc aucacagaaa gcaccgaaaa cuccaaaaug     1680

augcucgaac uagauccccc guucggagac agcuacaucg uaauaggcgu cggagagaag     1740

aaaauaacac accacuggca ccgauccgga uccacaauag gcaaagccuu cgaagcaacc     1800

gucagaggcg caaaacgaau ggccguacug ggagacacag ccugggacuu cggaagcgug     1860

ggcggagcac ucaacagccu uggcaaagga auccaccaaa ucuucggcgc agcauucaaa     1920

ucacucuucg gaggcauguc cugguucuca caaauacuga uagggacacu ccugaugugg     1980

uuaggucuca acaccaaaaa cggcagcauu ucucucaugu gccucgcccu cggaggagua     2040

cucaucuucc ucuccacagc ggucagcgca gacgucggau gcuccgucga cuuuucgaaa     2100


<210>  2777
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2777
gccgacacca gcgucggaau cgucgggcuc cuauugacca ccgccauggc cgccgaaguc       60

acccgccgcg gaagcgccua cuacauguac cuagaccgaa acgacgcagg cgaagccauc      120

agcuucccca caacacuagg caugaacaaa ugcuacauuc aaaucaugga ccuaggccac      180

augugcgacg caaccauguc auacgagugu cccaugcuag acgaaggagu agaaccagac      240

gauguagacu gcuggugcaa caccaccucc acaugggucg ucuacggaac cugccaccac      300

aaaaagggcg aggcacgacg aucacgccgc gccgucacuc uaccaucaca cucaacucga      360

aaacuacaaa cccgcuccca aacaugguua gaaagccgcg aguauacgaa acaccuaauc      420

cgaguagaaa auuggauauu ccggaaccca ggcuucgcac uggcagccgc cgcaaucgca      480

uggcuucugg gauccucaac gucacaaaaa gucaucuacc ucgucaugau acugcucaua      540

gcaccagcau auuccauccg cuguaucggg guauccaaca gggacuucgu ugaaggcaug      600

ucaggaggga caugggucga caucguccuc gaacacggag gcugcgucac agucauggca      660

caagacaaac ccaccguaga caucgagcuc gucaccacaa cagucagcaa cauggccgaa      720

guccgcagcu acugcuacga agcaucaauc agcgauaugg ccagcgacuc aaggugcccu      780

acucaaggcg aggcuuaccu agauaaacaa agcgacaccc aauacgucug caaacgcacc      840

cuaguagacc gaggaugggg aaacggaugc ggccucuucg gcaaaggcuc ccucgugaca      900

ugcgcaaaau ucgcaugcag caagaaaaug acaggcaaaa gcauacaacc agaaaaccuc      960

gaauacagaa ucaugcugag cguccacggg ucacaacacu caggcaugau agugaacgac     1020

accggccacg aaaccgacga aaaccgcgca aaaguagaaa uaaccccaaa cagcccacga     1080

gccgaagcca cccuaggcgg cuuuggaagc cucggccuag acugcgagcc ccgcaccggu     1140

cuagacuuca gcgaccucua cuaccugacc augaacaaca aacauuggcu cgugcacaaa     1200

gagugguucc acgacauacc ccuaccuugg cacgcaggag cagacaccgg cacaccccac     1260

uggaacaaca aggaggcacu gguagaguuc aaggacgccc acgcaaaacg acaaacagua     1320

gucgugcuag gaucucaaga aggcgccguc cacacagccu uagccggggc acucgaagca     1380

gagauggacg gagccaaagg acgacuaucc uccggccauc ucaaaugccg ccucaaaaug     1440

gacaagcucc gacuaaaagg cguaagcuac agccuuugca cggcagcauu cacguucacg     1500

aaaauucccg ccgaaacacu ccacggcaca guaaccgucg aaguacaaua cgccggcaca     1560

gaugggcccu gcaagguccc cgcccaaaug gcaguugaca ugcaaacccu cacccccgua     1620

ggccggcuga ucaccgccaa cccagucauc accgaaucca ccgagaacag caaaaugaug     1680

cucgaacucg aucccccuuu cggagacucc uacauaguca ucggaguagg agaaaagaaa     1740

aucacccacc acuggcaccg cagcggcucc accauaggaa aagcauucga agcuaccguc     1800

cgcggggcca aacgcauggc cguacucggc gacacggcgu gggacuucgg cucgguugga     1860

ggagcccuaa auagccucgg caaaggaauc caccaaauau ucggagcggc uuucaaaucc     1920

cuuuucggug gcaugucaug guuuagccaa auucucaucg gaacacugcu cauguggcug     1980

ggcuuaaaca ccaaaaaugg aucaaucucg cucaugugcc uagcacuagg agggguccua     2040

auauuccugu caaccgcagu gagcgccgac guaggcugca gcgucgacuu cucaaaa        2097


<210>  2778
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2778
gacacaucag uuggcaucgu cgggcugcua cucaccacgg ccauggccgc agaagugaca       60

agaagaggaa gcgcauacua cauguaucug gaucgcaacg acgccggcga agcgaucagc      120

uucccaacga cccucggaau gaacaaaugc uacauacaga ucauggaccu aggacacaug      180

ugcgacgcga ccauguccua cgagugccca augcuagacg aaggcgucga gccagacgac      240

gucgacugcu gguguaauac gaccucaacc uggguagucu acgguacaug ccaccacaag      300

aaaggcgaag cacgaagauc aagacgagcc gucacccuac ccucacacag cacuagaaaa      360

cuccaaaccc gaagucaaac auggcuagaa agucgagaau acacgaaaca ccugauccgc      420

guagaaaacu ggauauuccg aaacccaggc uucgcgcucg ccgcagccgc cauagccugg      480

cuccucggca gcagcaccag ucagaagguc auauaucucg ugaugauccu ccucaucgcc      540

ccugccuacu caauaagaug caucggcgua agcaaccggg acuucgugga aggaaugucu      600

ggaggcaccu ggguagacau aguccucgaa cacggaggcu gcguaacugu aauggcccaa      660

gacaaaccua cagucgacau ugaacugguc accaccaccg uauccaacau ggccgaagug      720

cgcagcuacu gcuacgaagc cagcaucuca gacauggcga gugacagccg augccccacu      780

caaggcgaag ccuaccuaga caaacaaagc gacacacaau acgucugcaa acgaacacuc      840

gucgaucgug gaugggguaa cggaugcggc cucuucggca aagguucccu agucacuugc      900

gcaaaauucg ccugcagcaa aaagaugaca ggaaagagca uacaacccga aaaccuagaa      960

uaccgcauca ugcugagcgu ccacggcagc cagcacagcg gaaugauagu aaacgacaca     1020

ggacacgaaa ccgacgaaaa uagggcgaaa gucgaaauca cccccaacag ccccagagcg     1080

gaagcaacac uagguggcuu cgggagccuc ggauuagacu gcgagccccg aacaggccuc     1140

gacuucagcg accuauacua ccuaacaaug aacaacaaac acugguuagu ccacaaagag     1200

ugguuccaug acaucccccu cccauggcac gccggcgccg acaccggcac accccacugg     1260

aacaacaagg aggccuuagu cgaguucaaa gacgcccacg caaaacgaca aacaguggua     1320

guccuaggaa gccaggaagg agccguccac acggcccucg caggagcacu cgaggccgaa     1380

auggacggcg caaaaggccg ccugagcuca ggacaccuca aaugccgccu aaaaauggac     1440

aaacuaagac ucaaaggagu aagcuacucc cuuugcacag cagccuucac auucacgaag     1500

aucccagcag aaacacugca uggcacagug acaguagaag uacaauacgc cggcaccgac     1560

ggaccaugca aagugcccgc ccagauggcc guagauaugc agacucuaac ucccguaggc     1620

agacuaauca ccgcaaaccc cgucaucacc gagagcacag aaaacagcaa aaugaugcua     1680

gagcucgauc ccccauucgg agacaguuac auaguaaucg gaguaggcga gaagaaaauc     1740

acccaccacu ggcaccgcag uggauccacu auaggcaaag ccuuugaagc aacaguccgc     1800

ggcgcaaaaa ggauggcagu ccuaggagac accgcauggg auuucggcag cguaggcgga     1860

gcacuaaaca gccucggcaa aggcauacac caaauauucg gagccgccuu caagagccua     1920

uucggcggca uguccugguu cagccaaaua cuaaucggca cccuccucau guggcucgga     1980

cugaacacca aaaacggguc aaucagccuc augugccugg cacugggagg gguacucaua     2040

uuccuaagca ccgcaguauc agccgacgua gguugcagcg uagacuuuag caaa           2094


<210>  2779
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2779
accucagucg gcauaguagg acugcuccug acaacagcga uggccgccga gguaacucga       60

agaggaagcg cguacuacau guaccucgac cgaaacgacg cgggcgaagc aaucagcuuc      120

ccgaccaccc uaggcaugaa caaaugcuac auccaaauaa uggaucuggg acacaugugc      180

gacgcaacca ugucauacga augucccaug cucgacgaag gaguagaacc cgacgacgug      240

gauugcuggu gcaacacaac cagcaccugg guagucuaug gaacaugcca ccacaagaaa      300

ggcgaagccc gacgauccag acgugccguc acgcuaccca gccacagcac acgcaaacuc      360

caaaccagaa gccaaacaug gcucgaaagc cgagaauaca ccaaacaccu cauaagaguc      420

gaaaacugga uauuccgcaa ccccggguuc gcacuagcug cagcagccau cgcauggcuc      480

cuaggcucca gcacaagcca aaaagucaua uaccuuguca ugauccuacu cauagcaccg      540

gccuacucca uacgcugcau aggcgucucc aaccgagacu ucgucgaagg aaugagcgga      600

ggcaccuggg uagauaucgu ccuggagcac ggcggaugcg uaaccgucau ggcgcaagac      660

aagccaaccg uagacaucga gcucguaacc accacgguua gcaacauggc cgaaguccgc      720

uccuacugcu acgaagcaag cauaagcgac auggcaagug acagcagaug ccccacucaa      780

ggcgaagcgu accucgacaa acaaagcgac acacaauacg uaugcaaacg aacccucgua      840

gacagaggcu ggggcaacgg cugcggccua uucggaaagg gcucccucgu aaccugcgcg      900

aaguucgccu gcucaaagaa aaugacaggc aaaucgauac aaccggaaaa ccucgaauac      960

agaauaaugc uguccgucca cggaucacaa cacagcggaa ugauagugaa ugacacagga     1020

caugaaaccg acgagaaccg agcaaaaguc gaaauaacac caaacucccc acgggccgaa     1080

gccacccucg gagguuuugg cagcuuaggc cucgacugcg agccacgaac gggccucgac     1140

uucucagacc ucuacuaccu aacgaugaac aacaaacacu ggcuugucca uaaagaaugg     1200

uuccacgaca uaccccuccc cuggcacgcc ggagccgaca ccggcacacc ccacuggaac     1260

aacaaagagg cgcuaguaga guucaaagac gcccacgcca agagacaaac cgugguagua     1320

cuagggagcc aagaaggcgc gguacacaca gcacuagccg gagcccucga agccgaaaug     1380

gauggagcaa aagggcgacu cagcuccggc caccucaaau gccgccuaaa aauggacaaa     1440

cuaagacuga agggcguaag cuacagccua ugcacagccg cauucaccuu caccaaaaua     1500

cccgccgaaa cccuacacgg aacagucacc gucgaagucc aauacgccgg uaccgacgga     1560

ccgugcaagg uaccggcaca aauggcagua gacaugcaaa cucuaacccc cguaggcaga     1620

cucaucacgg ccaaccccgu aauaaccgaa ucuacagaaa acaguaagau gaugcucgaa     1680

cucgacccac ccuuuggcga cuccuacauc gucauaggag ucggagaaaa gaaaaucacc     1740

caccacuggc acaggagugg cagcacaauc gguaaagccu ucgaagccac aguacgcggg     1800

gcaaaacgca uggcgguccu cggcgacaca gcaugggacu ucgggagcgu aggcggcgcg     1860

cuaaacagcc ucggcaaagg cauccaccag aucuucggcg cggcauucaa aucacuauuu     1920

ggagggauga gcugguucuc ucaaauccuc auaggaaccu ugcugaugug gcugggccua     1980

aacacgaaaa acgggagcau cagccucaug ugccuugccc uagguggcgu ccuaaucuuc     2040

cuuagcacag ccgucucagc cgacgucgga ugcagcgugg acuucaguaa a              2091


<210>  2780
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2780
accagcgucg guauaguagg ccuacuacua acaacggcca uggcagccgc cgugacacgc       60

agaggaucug ccuacuacau guaccuagac cgcaacgacg ccggcgaagc gaucuccuuc      120

ccuacuaccc ugggcaugaa caaaugcuac auccaaauca uggaccuagg acacaugugc      180

gaugccacaa ugucauacga augcccuaug cuggacgagg gagucgaacc cgacgacgua      240

gauuguuggu gcaacaccac aagcacaugg guaguauaug gaacaugcca ccacaaaaag      300

ggcgaagcgc gcagaagccg ccgcgccguc acccuaccca gccacuccac aagaaaacug      360

caaacgagaa gccaaaccug gcucgaaucc cgcgaauaca ccaaacaccu cauccgcguc      420

gaaaacugga ucuuccgaaa ccccggcuuc gcgcuagccg cggccgcuau cgcguggcuc      480

cugggaucaa guaccagcca aaaaguaauc uaccuaguaa ugauccuccu caucgcacca      540

gccuacucca uccgaugcau uggcguaagc aacagagacu ucgucgaggg caugaguggc      600

ggcaccuggg uagacaucgu gcuagaacac ggaggcugcg ucaccguaau ggcccaagac      660

aaacccacag ucgacauaga acucguaaca acaacgguuu caaacauggc agaggugaga      720

ucguauugcu acgaagccag caucagcgau auggccagcg acagccgcug cccgacacaa      780

ggagaagcgu accuggacaa acaaagcgac acccaauacg ucugcaagcg aacacucgua      840

gaccgcgggu ggggcaacgg cugcggacua uucggaaaag gaagccuugu aaccugcgca      900

aaguucgccu gcagcaagaa aaugacaggc aaaagcaucc aaccagaaaa ccucgaauac      960

cggaucaugc ucagcgucca uggcucacaa cacagcggca ugauagucaa cgacacgggc     1020

cacgaaacag acgaaaaccg agcaaaagua gaaauaaccc ccaacucccc aagagcggaa     1080

gccacccuag gcggauucgg aucccucgga cucgacugcg aaccacgaac cggacucgac     1140

uucucagacc ucuacuaccu caccaugaac aacaaacacu ggcucgucca caaagaaugg     1200

uuccacgaca uaccacuccc auggcacgcc ggcgccgaca caggcacacc acacuggaac     1260

aacaaagagg cccuugucga guucaaagac gcacacgcaa aacgccaaac cgucguaguc     1320

cucggauccc aagaaggagc gguccacacc gcccucgcag gcgcccuaga agccgaaaug     1380

gacggagcga aaggacgccu aucaucgggg caccucaagu gcagacucaa aauggacaaa     1440

cuccgacuaa aaggcgucag cuacucacuc ugcaccgccg cauucacguu caccaaaauc     1500

cccgccgaaa cccuacacgg aacagugaca guagaagugc aguacgcagg aacagacgga     1560

ccaugcaaag uccccgccca gauggcagua gacaugcaaa cacucacacc agugggacgc     1620

cuaaucaccg ccaaccccgu aaucacagaa agcaccgaaa acagcaaaau gaugcuagaa     1680

cucgacccac ccuucgggga cuccuacauc guaauaggag ucggggaaaa gaaaaucacg     1740

caccacuggc accgaagcgg uuccacaauc ggcaaagccu ucgaagcaac aguacgcggc     1800

gcaaaacgga uggcgguccu cggagacacc gcaugggacu ucggaagcgu cggaggcgcc     1860

cuaaacaguc uaggcaaagg aauacaccaa auauucggug cggccuucaa aagucuauuc     1920

ggcggcauga gcugguucag ccaaauccuc auaggaacac ucuuaaugug gcucggccua     1980

aauacuaaaa acggcucaau uagccuaaug ugcuuagcac uaggaggcgu gcucaucuuc     2040

cugucaaccg ccgucuccgc cgacguaggc ugcagcguag acuuuagcaa a              2091


<210>  2781
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2781
cgaggcgcag acacgagcgu aggcaucguc ggccugcuuu uaaccacagc aauggcugca       60

gccguaaccc gacgaggaag cgcauauuac auguaccuag acagaaauga cgcaggagaa      120

gcaauaucgu uccccacaac acuaggaaug aauaagugcu acauccaaau cauggaccuc      180

ggacacaugu gcgacgccac gaugucguac gaaugcccca ugcuagacga aggagucgaa      240

ccagacgacg uggauugcug guguaacaca accucaaccu gggugguaua cggcacgugc      300

caccacaaga aaggcgaagc aagacgauca agacgagcag uaacucuccc cucccacagc      360

accagaaaac uccagacaag aucccaaaca uggcuagaau cacgagaaua cacaaaacac      420

cugauaagag ucgaaaacug gauauucaga aaccccggau ucgcacucgc cgcagcagcc      480

auagcauggc ucuuaggcag cucaacaagc caaaaaguaa ucuaccuagu caugauccua      540

cuaaucgcgc ccgcguacuc gauccgcugc aucggcguau cgaacagaga cuucgucgaa      600

ggcauguccg gagggacgug ggucgacaua gugcuagaac acggcggaug cgucacaguc      660

auggcacaag acaaaccgac ggucgacaua gagcuaguaa ccaccacagu aagcaacaug      720

gcagaaguca gaaguuacug cuacgaagca uccauaucag auauggcauc agacuccaga      780

ugcccgaccc agggagaggc cuaccuagac aaacaaucag auacccaaua cgucugcaaa      840

cgaacccugg uagaccgcgg cuggggaaac ggcugcggcc uguucggcaa ggggagcuua      900

guaaccugcg caaaauucgc augcagcaag aaaaugaccg gaaaaagcau ccaacccgaa      960

aaccucgaau aucgcauaau gcucagcguc cacggcagcc aacacagcgg aaugauaguc     1020

aacgacacag gccacgaaac agacgaaaac agggcaaaag ucgaaaucac accaaauagc     1080

ccaagggccg aagcaacacu cgggggcuuc gggucacuug gccuagacug cgaacccaga     1140

acgggacuag acuucagcga ccuguacuac cucaccauga acaacaaaca cuggcuagua     1200

cacaaagaau gguuccacga cauaccccuc ccauggcacg caggagccga caccgggacg     1260

ccacacugga acaacaagga agcacucguc gaguucaaag acgcccacgc aaagcgccaa     1320

acaguagucg uacugggcuc ccaagaaggg gcaguacaca cggcacucgc aggcgcacuc     1380

gaagcagaaa uggacggcgc caaaggccgc cucucaagcg gacaccucaa augccgccuc     1440

aagauggaca agcuccgacu caaaggcgua agcuacagcc uuugcacagc agcauucaca     1500

uucacaaaaa uacccgcaga aacacuccac ggcacaguca cagucgaagu gcaauacgca     1560

ggaaccgacg gcccaugcaa gguaccagca caaauggcag ucgacaugca aacgcuaacc     1620

ccagucggcc gccucauaac cgcaaaccca guaauaacug agagcacaga aaacagcaag     1680

augaugcucg agcucgaccc acccuucggc gauagcuaca uagugauagg agucggcgag     1740

aaaaagauca cgcaccauug gcacagaagc ggaagcacaa uaggaaaagc guucgaagca     1800

acagucagag gcgcaaaacg cauggccguc cucggcgaca cagcauggga cuucggcuca     1860

guaggagggg cacucaauag ccuaggcaaa ggaauacacc agauauucgg agcggcauuc     1920

aaaagccugu ucggcgguau gagcugguuc agccagauac uaauuggaac ccuacuaaug     1980

uggcuaggcc uaaacaccaa aaacggaagc aucagccuaa ugugccuagc acuuggcgga     2040

guacucauau uccucagcac agcagucucc gccgacguag gaugcagcgu cgacuucagc     2100

aaa                                                                   2103


<210>  2782
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2782
cgagggcucu caucacgcaa acgccgcagu cacgacgucc uaaccgucca auuucuaauc       60

cuaggaaugc uccuaaugac cggagggguc acccgcagag gcucggccua cuacauguac      120

cucgacagga acgacgccgg cgaagcaauu agcuucccca caacccuugg uaugaacaaa      180

ugcuacauac aaauaaugga ccuaggacau augugcgacg caacgauguc cuacgagugu      240

cccauguugg acgaaggcgu agaaccagac gacguggacu gcuggugcaa caccacaagc      300

acauggguug uauacggcac augccaccac aagaaaggag aagcccgccg aucacgacgu      360

gccgucaccc ugcccagcca cagcacgcgc aaacuacaaa cucgcucgca aaccuggcug      420

gaaagccgcg aauacacaaa acaccucauc cgagucgaga acuggauauu ccgcaacccc      480

ggcuucgcac ucgcggcagc cgcaauagca uggcuucuag ggucaagcac cagccaaaaa      540

gucaucuacc ucguaaugau ccuccugauc gcccccgccu acuccaucag augcaucgga      600

guaucaaaca gagauuucgu agaaggcaug ucagguggaa ccuggguaga caucguacua      660

gagcacggag gcugcgucac cgucauggca caggauaaac ccacagucga caucgaacug      720

gucacgacca cugucucaaa cauggccgaa gugcguagcu acuguuacga agccagcauc      780

agcgacaugg cgagcgacag ucgaugcccc acccaagggg aagcauaccu cgacaaacaa      840

agcgacacac aauacguaug caagcgaaca cucguagacc gcggcugggg gaacggaugc      900

ggacucuucg gaaaaggcag ccuagugacc ugcgccaaau ucgcuuguuc aaagaaaaug      960

accggcaaaa gcauacaacc ggaaaaccuc gaauacagaa uaaugcucuc cgugcacgga     1020

ucucagcaca gcggcaugau agucaacgac accggccacg agaccgacga aaacagggcg     1080

aaagucgaaa uaacaccgaa cagcccccga gccgaggcga cacucggcgg cuucgggucc     1140

cucggccuag auugcgaacc ccgcacaggc cucgacuuca gcgaccucua uuaucuaaca     1200

augaacaaca aacacuggcu aguccacaaa gaaugguucc acgacauucc acuccccugg     1260

cacgcgggag cggacacggg aacaccacac uggaacaaca aagaagcacu cguagaguuc     1320

aaagacgcac acgccaaacg acaaaccgua guaguacuag gaagccaaga aggcgccgug     1380

cacacagcac uagcaggggc ccuagaagcc gaaauggacg gcgccaaagg ccgacuaagc     1440

aguggacacc uaaaaugccg ccuaaaaaug gacaaacuac gccuaaaagg agucagcuau     1500

agccuaugca cagccgcauu cacguucaca aaaauucccg cggaaacgcu acacggaacu     1560

guaaccgucg aaguacaaua cgccggcacc gacggaccgu gcaagguccc ugcacaaaug     1620

gcuguagaca ugcaaacacu cacaccgguc ggccgucuaa uaacagccaa ccccguaauc     1680

accgaaucca ccgaaaacuc aaaaaugaug cuggaacucg acccacccuu cggagacagu     1740

uacauaguca ucggagucgg agagaaaaag aucacucacc acuggcacag aucaggcuca     1800

acgaucggaa aagccuucga agcaacaguc cgcggggcaa aaagaauggc cguccuagga     1860

gacacagccu gggauuucgg uagcguagga ggagcccuca acucccuagg aaagggcaua     1920

caccaaauau ucggagccgc cuucaaaagc cucuucggag gcaugucgug guucucgcaa     1980

auacuaaucg gcacccuccu gauguggcua gggcuaaaca ccaaaaacgg aucgaucucu     2040

cucaugugcc uagcccucgg uggcgugcua auauuccucu ccaccgccgu cagcgcggau     2100

guaggaugca gcguagacuu cagcaag                                         2127


<210>  2783
<211>  2127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2783
cgcggacuca gcucccgcaa acgacgcuca cacgacguac uaaccgugca auuccucauc       60

cuugggaugc uacucaugac aggcgcggug acccgacgcg gcagcgcaua cuacauguac      120

cucgaccgaa acgacgcggg cgaagcaauc agcuucccca caacacucgg caugaacaaa      180

ugcuacauac aaauaaugga ccucggacac augugcgacg ccaccauguc guacgagugc      240

ccaaugcuag acgaaggcgu agaacccgac gacgucgacu gcuggugcaa uaccaccuca      300

acauggguag ucuacggcac augccaccac aagaaaggag aagcccgccg cucuagacgc      360

gccgucacuc uccccaguca cucaacccga aaacuccaaa cgcgcuccca aacauggcuc      420

gagucccgcg aauacacaaa acaccucauc cgaguagaaa acuggaucuu ccgaaacccc      480

ggauucgcac ucgccgcagc agccauagca uggcuccugg gaucaagcac aucccagaaa      540

guaaucuacc ucgucaugau ccuacuaauc gcucccgcgu acagcauacg augcauaggc      600

gucagcaaca gagacuucgu agaaggaaug ucgggcggca cauggguaga caucgugcuc      660

gagcacggcg gaugcguaac cguaauggcc caagacaaac ccacaguaga caucgagcuc      720

guaacuacca cagucaguaa cauggccgaa guccgcagcu acuguuacga ggccucaauu      780

ucagacaugg ccuccgacag ucgcugccca acacaaggcg aagcguaccu ggacaaacaa      840

uccgacaccc aauacguaug caaacgcacc cucguugaca gggggugggg aaauggaugc      900

ggauuauucg guaaaggguc ccuaguaacc ugcgccaaau ucgcaugcuc aaagaaaaug      960

accggcaaau caauacaacc agagaaccua gaauaccgca uaaugcucag cguccacggg     1020

agccaacauu ccggaaugau cguaaacgac accggacacg aaaccgauga gaaucgcgca     1080

aaaguagaaa ucacaccaaa cuccccacgg gcugaggcga cacuaggcgg cuucggcagc     1140

cuaggccuag acugcgaacc ccgcaccggg cucgacuucu cggaccugua cuaccucacg     1200

augaacaaca aacacuggcu cguacacaaa gaaugguucc acgauauccc ccucccaugg     1260

cacgccggcg ccgacaccgg aacaccccac uggaacaaca aagaagcccu cguagaguuc     1320

aaagacgccc acgccaaaag acaaacagug guuguacuag ggucacagga aggcgccgua     1380

cacacagcac ucgccggagc ccucgaagcc gaaauggacg gggcaaaagg uagacuaagu     1440

agcggccacc ucaagugccg acucaaaaug gacaagcugc gacugaaagg ggucaguuac     1500

ucccuaugca ccgcugcauu caccuucacc aagaucccgg ccgaaacccu acacggaaca     1560

gucaccgucg aagugcagua cgccggcaca gacggccccu gcaaaguacc cgcgcaaaug     1620

gccgucgaca ugcaaacacu cacgcccguc ggacggcuca ucacagccaa ccccguaauc     1680

acggaaagca cagagaacag caaaaugaug cuagaacuag acccacccuu cggagacucc     1740

uacauaguaa ucggcguagg cgagaagaaa auuacacacc acuggcacag guccggcuca     1800

acgauuggua aagccuucga agcaacaguc cgcggugcca agcgcauggc aguccuaggc     1860

gacacagcau gggacuucgg cucagucggc ggcgcacuca acagucuggg aaagggcaua     1920

caucagauau ucggcgcagc cuucaagucg cuauucggag ggaugagcug guucagccag     1980

auccucaucg gaacacugcu cauguggcua ggacuaaaca ccaaaaacgg uagcaucagc     2040

cucaugugcc uagcacuagg cggaguccua auauuccuua gcacggcagu cagcgccgac     2100

gugggcugca gugucgacuu cucuaaa                                         2127


<210>  2784
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2784
cgcaucauca acgcgcgcaa agaaaagaaa cgacgcggag ccgacaccuc ggucggaaua       60

gucggccucc uacugacaac cgccauggca gccgagguca cccgacgagg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccgga gaggccauuu ccuuccccac cacccucggc      180

augaacaaau gcuacaucca aauuauggac cuaggacaca ugugcgacgc aacaaugagc      240

uacgaaugcc caaugcuaga cgaaggugua gaacccgacg acguagacug cuggugcaac      300

acaacaucca caugggucgu guacgguaca ugccaucaca agaaaggcga agcacgccgc      360

agccgacgcg ccguaacccu cccaucccac agcacaagga aacuucaaac acgcagccag      420

acguggcucg aaagcagaga auacacaaaa caccucauac gaguagaaaa cuggauauuu      480

cgcaacccgg gcuuugcccu cgccgccgcg gccauagcau ggcuauuagg cagcaguacg      540

ucccaaaaag uaauuuaccu agucaugaua cuacuaauag caccagccua cuccauaagg      600

ugcaucggag ugucaaaccg cgacuucguc gaaggaauga gcggaggaac augggucgac      660

aucguccucg aacacggcgg uugcgucaca gucauggccc aagacaaacc cacaguagac      720

aucgaacucg ucacgacaac aguauccaau auggccgaag uacgauccua cugcuacgag      780

gcaagcauau cagacauggc cuccgacuca agaugcccaa cccaaggaga agccuaccuc      840

gacaaacagu ccgacacuca auacgugugc aaacggacac uugucgaccg cggguggggc      900

aacggaugug gccuauucgg caaaggcagu cugguaaccu gcgcaaaauu ugcaugcucc      960

aagaaaauga cagggaaauc cauacaaccc gaaaaccucg aauacagaau caugcucuca     1020

guccacgggu cacagcacag cggcaugaua guaaacgaca ccggccacga gacagacgaa     1080

aaccgcgcca aaguagaaau aaccccaaac agcccccggg ccgaggccac ccugggcggg     1140

uucggcagcc ucggucucga cugcgaaccc cgaacagguc uagauuucuc agaccuauac     1200

uaccuaacca ugaacaacaa acauuggcuc guacauaaag agugguucca cgacauccca     1260

cucccauggc acgcaggcgc ggacaccggu accccacacu ggaacaacaa agaagcgcua     1320

gucgaguuca aagacgcaca cgcaaaaaga caaacagucg uaguccuagg cucccaggaa     1380

ggagccgucc acacggcacu cgccggagca cucgaggcag agauggacgg cgccaaaggc     1440

cgccucagcu caggacaccu aaaaugccgc cucaaaaugg acaaacucag acucaaaggg     1500

guaucauaca gccuaugcac agccgcauuu acguucacca aaauaccagc cgagacacuc     1560

cacggcaccg uaacagucga gguccaauac gcaggaacag acggccccug caaaguccca     1620

gcccaaaugg ccgucgacau gcaaacacuc acacccgugg gccgucuaau aaccgcaaac     1680

cccguuauaa cagaauccac agaaaacucc aaaaugaugc uggaacucga ucccccauuc     1740

ggggacagcu acaucguaau cggcguaggc gaaaagaaaa ucacccacca cuggcaccgc     1800

uccggaagca caauaggcaa agcauucgaa gcaaccgucc gcggagcgaa aagaauggcu     1860

guacucggag acaccgcgug ggacuucggc agcgugggcg gcgcacucaa cucccuagga     1920

aaaggcaucc accagauauu cggcgcagcc uucaaauccc uuuucggcgg cauguccugg     1980

uucucccaaa uccuuauagg aacccuucua auguggcucg gccucaacac caaaaacggc     2040

uccauauccc ucaugugccu cgcccucggc ggcguacuaa ucuuccuaag cacagccguc     2100

agcgccgacg ucggaugc                                                   2118


<210>  2785
<211>  2115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2785
aucaucaacg cccgaaaaga aaagaaacga cgaggcgccg acacaagcgu aggcaucgua       60

ggacuccugc ucacuacagc cauggcagca gaagugaccc gccgcggauc cgccuacuac      120

auguaccucg accgcaacga cgccggagag gcaauaucau uccccaccac gcuaggaaug      180

aacaaaugcu acauacaaau aauggaucuc ggacacaugu gcgacgccac caugagcuac      240

gaauguccaa ugcuagacga gggaguugaa cccgaugacg uagacugcug gugcaacacc      300

acaucgaccu gggucgucua cgguaccugc caccacaaga aaggcgaagc ccgacgcuca      360

cgccgagccg ucacucuacc cagccacucc acaagaaaac uccaaacacg cagccaaaca      420

uggcuagaau cccgcgaaua cacaaaacac cuaauacgag uagagaacug gaucuucaga      480

aaccccggcu ucgcgcucgc agcggccgcc aucgcauggc ucuuaggcuc cagcaccagc      540

cagaaaguua ucuaccucgu aaugauccuc cucaucgccc cagccuacuc cauccgaugc      600

auuggaguaa gcaacaggga cuucguagaa ggaaugagcg gcggaacaug ggucgacaua      660

guacucgaac acggaggcug cguaaccgua auggcacaag acaaacccac cguggacaua      720

gaacucguca caaccaccgu uuccaacaug gccgaaguga gaagcuacug uuacgaagcc      780

agcaucucag acauggcuuc cgauagcaga ugccccaccc aaggagaggc auaccucgau      840

aaacaaucag acacccaaua cgucugcaaa agaacacucg uagaccgcgg uuggggcaac      900

ggcugcggac ucuuuggcaa aggaucccua guuaccugcg caaaauucgc cugcagcaaa      960

aagaugacgg gcaagucaau acagcccgaa aaucuggaau accggaucau gcuaucgguc     1020

cacggcagcc aacacagcgg caugaucgug aacgacaccg gccacgaaac cgacgaaaac     1080

agagcaaaag ucgagauaac cccaaacuca ccccgagcag aagccacccu aggcggauuc     1140

ggcagccugg gccuagacug cgaacccaga accggccuag acuucuccga ccuguacuac     1200

cucacaauga acaacaaaca uugguuagua cacaaagaau gguuccacga cauaccucua     1260

ccauggcacg ccggcgcaga caccgggacc ccgcacugga acaacaagga agcacuggua     1320

gaguucaagg acgcacacgc aaaacgccaa acggucgucg ugcucggcag ccaagaagga     1380

gccguccaca ccgcgcuagc aggagcucuc gaggcggaaa uggacggcgc caaaggcagg     1440

cucuccucag ggcaccucaa augccgccuc aaaauggaca aacuccgacu caaaggugug     1500

uccuacucuc ucugcacagc agccuucaca uucaccaaga ucccugccga aacacuccac     1560

gggacaguca cgguagaagu ccaauacgcc gguacagacg gaccgugcaa agucccagcc     1620

caaauggccg uagacaugca gacacuuacc ccagucggcc gccucaucac cgccaacccc     1680

gucaucacag aaagcaccga aaacucaaaa augaugcugg aacucgaccc acccuucggu     1740

gacagcuaca ucguaaucgg aguaggagag aagaaaauaa cacaccacug gcaucgcagc     1800

ggcuccacca ucggcaaagc auucgaggcu acaguaagag gagccaaacg aauggccguc     1860

cuaggagaca ccgcauggga cuucgggagc gucggcggcg cacuaaacag ccucggaaaa     1920

gggauacauc aaaucuucgg cgccgccuuu aaaucauuau ucggcggcau gagcugguuc     1980

agccaaauau uaaucggaac gcuacucaug uggcuagggc ucaacaccaa aaacggaucc     2040

aucucacuaa ugugccuggc ucucggaggc guccucaucu uccuuucaac agcaguaagc     2100

gccgacguag gcugc                                                      2115


<210>  2786
<211>  2112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2786
aucaacgccc gcaaagagaa gaaacgcaga ggcgccgaca cgagcguagg aaucgucggc       60

cuacuacuca cuacagccau ggcggccgag gucacacgca gagggucagc cuacuacaug      120

uaucucgaca ggaacgacgc cggagaagcg aucucauucc ccaccacccu cggaaugaac      180

aaaugcuaca uccaaauaau ggaccucggg cacaugugcg acgcuacaau guccuaugag      240

ugcccuaugc uagacgaagg cguggaaccu gacgacguag auugcuggug caacacaacu      300

ucaaccuggg uaguauacgg cacaugccac cacaagaaag gagaggcacg caggagccgg      360

cgcgccguca cgcucccauc acacuccacg cgaaaacuuc aaacccggag ccaaacuugg      420

cuagaaagca gagaauacac caaacaccuc auaagaguag aaaacuggau cuuccgaaac      480

cccggauucg cgcucgcagc cgcagccauc gccuggcuac ucggcucgag cacaagccaa      540

aaagugaucu accuaguaau gauacuccua aucgcccccg ccuacucaau ccgaugcauc      600

ggaguaucaa accgagacuu cguagaagga augucaggag gaacuugggu cgauauagua      660

cuagaacacg gaggcugcgu cacggucaug gcccaagaca aaccaacugu ugacaucgaa      720

uuaguaacca cgaccgucag caauauggca gaggugcgau ccuacugcua cgaagccucc      780

aucucggaca uggcaagcga cucacgaugc ccaacccagg gagaggcgua ccucgacaaa      840

caaagcgaca cacaguacgu augcaaaagg acgcuagucg accgcggaug ggggaacgga      900

ugcgggcucu uuggaaaagg cucgcucgua acaugcgcca aauucgcgug caguaaaaag      960

augacaggca agagcauaca accagaaaac cuagaauacc ggaucaugcu cuccguccac     1020

ggcucccaac acagcggaau gauagucaac gacaccggac acgaaaccga cgagaacaga     1080

gcaaaagucg aaauaacacc caacagcccc cgcgccgaag caacccuagg aggguucggg     1140

agccucggcc uggacugcga accacgcacc ggacuagacu ucagcgaccu auacuaccua     1200

acaaugaaca auaaacacug gcuaguccac aaagaguggu uucacgacau accccugcca     1260

uggcacgcag gagcugacac cggaacccca cacuggaaca acaaagaagc ccuggucgag     1320

uucaaggacg cccacgccaa aagacaaaca guagucguac ucggcucaca agaaggcgcg     1380

guacacacag cacucgccgg cgcgcucgaa gccgagaugg acggcgccaa gggcagauua     1440

agcagcggcc accucaagug caggcuaaaa auggacaaac uacgacugaa gggcguauca     1500

uacucacucu gcacagcugc auucacauuc accaagauac cagcggaaac acuacacggg     1560

acaguaaccg uagaaguaca auacgccggc accgacggcc ccuguaaggu cccggcccaa     1620

auggcagucg acaugcagac ccuaaccccu gucggaagac ugaucaccgc caacccagua     1680

aucacagaau caacagagaa cucaaaaaug augcuggaac uggacccgcc cuucggagau     1740

agcuacaucg ugaucggcgu cggugaaaag aaaaucacgc accacuggca cagaagcggg     1800

agcaccauag guaaagccuu cgaggcgaca guuaggggcg ccaaacgaau ggcaguccuc     1860

ggcgacacag caugggacuu cggcagcgug ggcggagcac ucaacucacu agggaaagga     1920

auccaccaaa uauucggcgc agcauuuaaa agccuauucg gaggaaugag cugguucuca     1980

cagauccuaa uuggcacacu acuaaugugg cuagggcuca acaccaaaaa cggaagcauc     2040

agccuuaugu gccucgcacu cgguggaguc cuaauuuuuc ugagcaccgc cgucagugca     2100

gacgugggau gc                                                         2112


<210>  2787
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2787
aacgcacgca aagaaaagaa gcgccgcgga gccgacacau ccguaggaau cguaggacua       60

cuccucacaa cggccauggc agccgaagua accagacgcg gcuccgccua cuacauguac      120

cucgaccgaa acgacgcagg agaggcuauc ucauucccca caacccucgg aaugaacaaa      180

ugcuauauac agauaaugga ccugggccac augugcgacg caacaaugag cuacgaaugc      240

ccaauguuag augaaggcgu agaacccgac gacgucgacu gcugguguaa caccacauca      300

accuggguag uauacggcac augccaccac aagaaaggcg aagcgcgcag aagccgucga      360

gcagucacac uaccaucaca cucaacacga aaacuucaga cccgaucaca aaccuggcua      420

gaaucacgag aauauacgaa acaccucauc cgaguagaaa acuggaucuu cagaaacccc      480

ggcuucgccc uugcagcggc ggccaucgcc uggcuccuag ggaguucaac cagccagaag      540

guaaucuacc ucgucaugau ccuccucauc gcaccagccu acagcauccg augcaucggc      600

gucagcaaca gagacuucgu agaaggaaug agcggaggca cauggguaga cauaguacuc      660

gaacacggcg ggugcguaac cgugauggca caagauaaac ccacagucga caucgaacuu      720

gugaccacca ccguaagcaa cauggcagaa guaagguccu auugcuacga agcaucaauu      780

ucugacaugg cauccgacag ccgaugccca acgcagggag aagccuaccu agacaaacaa      840

agcgacaccc aauacguaug caaacgaacg cugguagacc gcggcugggg caacgguugc      900

ggacuauucg ggaaaggaag ccuagucaca ugcgccaaau ucgccugcag caaaaagaug      960

acaggcaaau caauccaacc cgaaaacuug gaauacagaa ucaugcuauc cguccacgga     1020

agccaacacu ccggcaugau agucaacgac acgggacacg aaacagacga aaaccgugca     1080

aaaguagaaa ucaccccgaa uagcccaaga gcggaagcca cacuaggggg cuuuggcagc     1140

cucggccuag auugcgaacc acgaacaggg cuagauuucu cggaccuaua cuauuuaacc     1200

augaacaaca aacacuggcu aguucacaaa gaaugguucc acgacauacc gcucccgugg     1260

cacgccggcg cagauacggg caccccacac uggaacaaca aggaagcgcu gguagaguuc     1320

aaagaugcuc acgccaaacg ccaaaccguc guaguacuag gcucacagga aggugcagua     1380

cacaccgcac uagccggagc acucgaagcc gaaauggacg gagcaaaagg acgacucagc     1440

agcggacauc uaaaaugucg acucaaaaug gacaaacuca ggcucaaggg agucagcuac     1500

agccuaugca cggccgcauu cacauucacc aaaauaccgg cagaaacacu ccacgguacc     1560

guaacaguag agguccaaua cgccggcacc gacggccccu gcaaaguacc agcacaaaug     1620

gcaguagaca ugcaaacccu cacccccgua ggacgacuaa uaacggccaa cccaguaaua     1680

acugaaagca ccgaaaacag uaagaugaug cuagaacugg acccaccauu cggugacuca     1740

uacaucguca ucggcguagg cgaaaagaaa auaacccacc acuggcaccg aagcggguca     1800

accauaggaa aagccuucga agcgacagug cgcggagcca aacgcauggc cguacugggc     1860

gacacagcau gggacuucgg aucaguaggc ggcgcccuca acagccucgg caaagggauc     1920

caccaaaucu ucggagccgc guuuaaaucg cuauucggcg gcauguccug guucucccaa     1980

auccucaucg gcacccuacu cauguggcuu ggccuaaaca ccaaaaaugg cucaaucagc     2040

cucaugugcc uagcacucgg aggcguccug aucuuccugu caacagcggu cucggcagac     2100

gucggcugu                                                             2109


<210>  2788
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2788
gcacgcaagg aaaagaagcg ccgcggagca gacaccucag ucggaauagu cggccugcua       60

cucaccaccg ccauggcggc ggaaguaacc cgccgggggu cagccuauua cauguaccuc      120

gaccgcaacg acgcaggcga agccaucuca uuccccacca cgcuaggcau gaacaaaugc      180

uacauccaaa uaauggaccu cggccacaug ugcgacgcca ccaugagcua cgaaugccca      240

augcuagacg aaggcguaga acccgacgac gucgauugcu ggugcaacac aacaucaaca      300

uggguugucu acggaaccug ccaccauaag aaaggcgaag cacgacgcag ccgcagagca      360

guuacacuac ccucccacuc gacaagaaaa uuacaaacac gaucacaaac cuggcucgaa      420

ucacgagaau acaccaaaca ccuaauccgc gucgaaaacu ggauauuccg aaaucccgga      480

uucgcucuag cagcagccgc gaucgccugg cugcucggcu ccuccacauc ccaaaaaguc      540

auuuaccucg ucaugauccu auuaaucgca ccggccuacu caauacgcug cauaggagua      600

uccaacagag acuucgucga agggauguca ggcggcaccu ggguagacau cguacuagaa      660

cacggcggcu gugucaccgu aauggcccaa gacaaaccaa ccgucgacau cgaacucguc      720

acgaccaccg uaagcaacau ggccgaagua cgcuccuauu gcuacgaagc aagcauauca      780

gauauggcaa gcgacucccg cugcccaaca cagggcgaag cauaccugga caaacaaagu      840

gacacccaau acgucuguaa gcggacccuc gucgaccgcg gauggggcaa cggcugcggc      900

cucuucggaa aaggaucccu aguaacaugc gccaaauucg caugcuccaa gaaaaugacc      960

ggcaagucca uacaaccaga aaaccuagaa uaccggauca ugcuaagugu acacggcagc     1020

caacauuccg gaaugauagu caacgacaca gggcacgaaa ccgacgaaaa ccgagcaaaa     1080

guagaaauaa ccccaaacuc ccccagagca gaagccacac uaggcggcuu uggcucucua     1140

ggccucgacu gcgaaccaag aacaggucua gacuucucag accuuuacua ccuaacaaug     1200

aacaacaaac acuggcucgu acacaaagaa ugguuccacg acaucccauu gccauggcac     1260

gcaggagcag acacaggaac accccacugg aacaacaaag aagcgcuagu cgaguucaaa     1320

gacgcacacg ccaagagaca aaccguagua guccucggcu cacaagaagg ugccguccac     1380

accgcacuag caggcgcacu agaagccgaa auggacggag caaaaggacg acuauccuca     1440

ggacaccuaa aaugccgucu caaaauggau aaacuaagac ugaaaggcgu aucauacuca     1500

cucugcacag cagccuucac guucaccaaa auccccgccg aaacacuaca cggcacaguc     1560

acaguagaag uacaauacgc cggcaccgac ggucccugca agguaccagc ucaaauggcg     1620

gucgacaugc aaacccuaac ccccguaggu agacucauca cugcaaaccc cgugaucacc     1680

gaaucaaccg aaaacucaaa aaugaugcug gaacuagacc caccguucgg cgacagcuac     1740

auagucaucg gaguagggga gaaaaagaua acccaccacu ggcacagaag uggaucgacc     1800

aucggcaaag ccuucgaagc aacaguucga ggcgccaaaa gaauggcagu ccucggagac     1860

accgcguggg acuucggcuc cguaggcgga gcccucaacu cccucggcaa gggaauacau     1920

cagaucuucg gcgccgccuu caagagccuu uucggaggaa ugagcugguu cucacaaauc     1980

cuaauaggca cacuacucau guggcucggc cucaacacca aaaacggguc caucucccuu     2040

augugccugg cacugggcgg aguacucauu uuccucagca cugccgucag cgccgacguc     2100

ggcugu                                                                2106


<210>  2789
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2789
agaaaagaaa aaaaacgccg aggagcagac acaagcgucg gcaucguagg auuacuacug       60

acaaccgcga uggcagccga agucacaaga cgaggcucag cauacuacau guaccucgac      120

cgcaacgacg caggugaagc aauaagcuuc ccgacgacuc uaggaaugaa caaaugcuau      180

auucaaauaa uggaccuagg acacaugugc gacgcaacca ugagcuacga augccccaug      240

cuagacgagg gugucgaacc cgacgacgua gacugcuggu gcaacaccac aagcaccugg      300

gucgucuacg gcacaugcca ccacaaaaaa ggagaggcac gcagaagcag aagagcaguc      360

acgcuccccu cccacagcac aagaaaacua caaaccagau cccaaaccug gcuggagucg      420

agagaauaca caaaacaccu cauaagggua gaaaacugga ucuucagaaa cccgggauuc      480

gcccuagcgg cagccgccau cgcauggcuc cugggcucaa guacuagcca gaaagugaua      540

uaccuaguaa ugauacugcu aauagcaccc gccuacucaa uccgcugcau cggcgucuca      600

aaccgagacu ucgucgaagg uaugagcggc gguacauggg uagacaucgu acuagaacau      660

ggcggaugcg ucaccgucau ggcacaggac aaacccacag uggacaucga acuggucacc      720

acaaccgucu cuaacauggc agagguacgc uccuacugcu acgaagccuc aaucucagac      780

auggcgagcg acucccgcug ucccacccag ggcgaagccu accuggacaa acaauccgau      840

acacaauacg ugugcaaacg cacacucguu gaccgagggu gggggaacgg uugcggccuc      900

uucggcaaag gcagccuggu gaccugcgca aaauucgcgu gcucgaaaaa aaugacaggc      960

aagagcauac aaccagaaaa ccuagaguac cggaucaugc uaucagucca cggaucgcaa     1020

cacucaggaa ugauagucaa cgauacagga caugaaaccg acgaaaaccg agcgaaagua     1080

gaaaucacac ccaacagccc gcgcgccgaa gcaacacuag ggggcuucgg aagccucgga     1140

cuagacugcg aaccacgcac cggccuagac uucagcgacc uuuacuaccu aaccaugaac     1200

aacaaacacu ggcuagucca caaagaaugg uuccaugaca ucccgcuacc guggcacgcu     1260

ggcgcagaca cggggacccc acauuggaac aauaaagaag cacuagucga auucaaagac     1320

gcgcacgcaa aacggcaaac agucgucgua cuaggcagcc aagagggagc uguccauacc     1380

gcgcuagccg gagcccuaga agcagaaaug gacggagcaa aaggccgccu uagcuccggc     1440

caccucaaau gucggcuuaa gauggacaaa cucagacuca agggcgucag cuacagccuc     1500

ugcaccgcag ccuucacauu caccaaaauc ccggccgaaa cccuacacgg aacaguaaca     1560

guagaggugc aauacgcggg aacagacggu ccaugcaaag ucccagccca aauggcugua     1620

gacaugcaaa cccuuacccc gguagggaga cuaauaacag ccaaccccgu aauuacugaa     1680

ucaacagaaa acagcaaaau gaugcucgaa cucgacccac ccuucggcga uagcuacaua     1740

guaaucgggg ucggagaaaa aaaaauaaca caccacuggc acagaucagg auccacaaua     1800

ggaaaagcau ucgaagccac cguuagggga gcaaaacgaa uggcaguacu cggcgacacc     1860

gccugggacu ucggcagcgu aggcggcgcc cuaaacagcc ucggaaaagg aauccaccaa     1920

auauucggug cagcauucaa aagccucuuc ggcggcauga gcugguucag ccaaauacuc     1980

aucggcacgc uccuaaugug gcuaggacua aauacaaaaa acggcaguau cagccuaaug     2040

ugccucgcgc uagggggcgu ccuaaucuuc cucucaacag cagucagcgc cgacgucgga     2100

ugc                                                                   2103


<210>  2790
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2790
aaggaaaaaa aacgacgcgg agcagacaca ucagucggaa ucguaggccu ccuccugaca       60

acagcaaugg cugccgaagu aacgaggcgg ggaagcgcgu acuacaugua ccucgacaga      120

aacgacgcag gcgaagccau cuccuuccca accacauuag gaaugaauaa augcuacauc      180

caaauaaugg accucggaca caugugcgac gccacaaugu cauacgaaug cccgaugcuc      240

gaugaaggcg ucgaacccga ugacguagac ugcuggugca acacaacgag cacauggguc      300

gucuacggaa caugccacca caaaaaaggc gaggcccgac gaucacgccg agcagugaca      360

cuccccuccc acagcacccg caaauuacaa acgagaagcc aaacauggcu cgaaagccgc      420

gaauauacca aacaccugau cagaguggaa aacuggauau ucaggaaccc cggcuucgcc      480

cuggcagcag cagcaauagc cuggcuacuu ggcaguagca caucccagaa agucaucuac      540

cucgucauga uacuccucau cgcaccugca uacagcaucc gaugcaucgg cguguccaac      600

agagacuucg uagaaggcau gagcggcgga acaugggucg acauaguacu cgaacacggc      660

ggcugcguaa ccgugauggc ucaagacaaa cccacagucg acaucgagcu agucaccacg      720

accguaagca acauggcaga aguacgcagc uacugcuaug aagccucgau auccgacaug      780

gcaucagacu cccggugccc cacccaggga gaagcauacc ucgacaaaca auccgacacc      840

caguacguuu guaaacgcac acuagucgac agaggcuggg gaaacggcug cggccuauuu      900

ggcaaaggca gccuggucac augcgcaaaa uucgccugca gcaaaaaaau gaccggaaaa      960

agcauacaac cagaaaaccu ggaauaccgc auaaugcugu ccguccacgg aagccaacac     1020

ucaggcauga ucguaaacga caccggccac gagaccgacg aaaacagagc aaaagucgaa     1080

aucacaccga acagcccacg agccgaagca acccucggag gguucggcuc guuaggacuc     1140

gacugcgaac caagaaccgg acucgacuuc ucagaccucu acuaccuaac aaugaacaac     1200

aaacacuggc ucguccacaa agaaugguuc cacgacauac cacucccaug gcacgccgga     1260

gccgacaccg gcaccccaca cuggaacaac aaagaagccc ucgucgaauu caaagacgcc     1320

cacgccaaac gacaaaccgu aguaguccuc ggaagccaag aaggcgcugu acacacggcc     1380

cucgcagggg cgcuggaagc cgaaauggau ggcgcaaaag gaagacuaag cuccggccac     1440

cuaaaaugcc gucucaagau ggacaaacug cgccucaaag gcgucucaua uagccuaugc     1500

accgccgcuu ucacauucac aaaaauaccc gccgaaacac uccacggcac aguaacaguc     1560

gaaguacaau augccggcac agacggccca ugcaaagucc cagcccaaau ggccgucgac     1620

augcaaaccc ucacaccagu cggccgccua aucacagcaa accccguaau aacagaaagc     1680

acagaaaacu caaaaaugau gcuagaacug gacccccccu ucggcgauag cuacauaguc     1740

aucggaguug gcgaaaaaaa aauaacacac cacuggcacc gcuccggcuc cacgaucggc     1800

aaagcauucg aagcaacggu acgaggagca aaaagaaugg caguacuagg agauacugca     1860

ugggacuucg gcuccguggg gggggcacua aacucccuag gcaaaggcau ccaccaaauc     1920

uucggcgcag ccuucaaauc acucuucggc gggauguccu gguucagcca aauccucauc     1980

ggcacccuac uaauguggcu ugggcuaaau acgaagaacg gaagcauauc ccucaugugc     2040

cuagcacuag gcggcguccu caucuuccuu agcaccgcag uauccgcaga cguaggcugc     2100


<210>  2791
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2791
gaaaagaaaa gacgaggagc agacaccagc gucggcaucg ucggucuacu acucacaaca       60

gccauggccg cagaagugac cagacgggga ucggcauacu acauguaccu cgaccguaac      120

gacgcaggag aagcaaucuc auucccaacc acacucggca ugaacaaaug cuacauccaa      180

auaauggacu uaggacacau gugcgacgcc acgauguccu acgaaugccc gaugcuagau      240

gaaggagugg aacccgacga cgucgacugc uggugcaaca ccacgagcac guggguagua      300

uacggaacau gucaccacaa aaagggugaa gccagacgcu cccgacgggc ggucacccuu      360

cccucacaca gcacaagaaa acugcaaacu cgcagccaaa cauggcugga aucacgcgaa      420

uacacaaaac accuuauacg ggucgaaaac uggaucuucc gaaacccagg auucgcacua      480

gcagcggccg ccauagccug gcuacuaggc agcaguacuu cacaaaaagu aauauaccua      540

gucaugauac uccugauagc ccccgcuuac uccauccgau gcaucggagu aagcaauaga      600

gauuucguug aaggaauguc aggaggaaca uggguagaca uaguccucga gcacggcggc      660

ugcguaaccg ucauggcaca ggacaaacca accguggaca uagaacuggu cacaacaacu      720

gucagcaaca uggccgaggu acgcaguuac ugcuacgagg cauccaucuc cgacauggca      780

ucggacagcc gaugcccgac ccaaggagag gcauaccucg acaaacaaag cgacacgcaa      840

uauguaugua aacgaaccuu aguagaccgc ggauggggca acgguugcgg acucuucgga      900

aaaggcuccc uggucacaug cgcaaaguuc gccugcucca agaaaaugac gggaaaaagc      960

auccaaccgg aaaaccuaga guaccgaauc augcugucug uccacggauc ccagcauucc     1020

ggcaugaucg ucaacgacac uggacacgaa acagacgaaa accgagccaa aguggagaua     1080

accccaaaca gcccaagagc agaggcaacc cuaggaggau ucggaucccu aggacuggac     1140

ugcgaaccgc gcacuggacu cgacuucuca gaccuauacu accuuacaau gaacaacaaa     1200

cacuggcucg uccacaaaga gugguuccac gauauccccc uacccuggca cgccggcgca     1260

gacacaggaa caccacacug gaacaacaaa gaagcccuag ucgaguucaa agacgcgcac     1320

gccaaacggc aaacaguagu cguccucgga agccaagaag gggcuguaca uaccgcacuc     1380

gccggcgccc uagaagccga aauggacggc gcgaaaggac gacuaagcuc aggacaccuc     1440

aaaugccggc ucaaaaugga caaacucaga cucaaaggag uuuccuacuc ccuaugcaca     1500

gcagccuuua ccuucaccaa aauacccgcg gaaacccucc acggaaccgu cacagucgaa     1560

guccaauaug cgggaaccga cgggcccugc aaagugcccg cccaaauggc cgucgauaug     1620

caaacccuca cccccguagg ccgacuaauc acagccaacc ccguaauaac cgagucaaca     1680

gaaaacucca aaaugaugcu agaacuagau cccccauucg gagacagcua cauaguaauc     1740

ggcguaggcg aaaagaaaau cacccaccac uggcaccgau ccggaagcac aaucggaaaa     1800

gccuucgaag caaccguccg uggcgccaaa agaauggccg uacuuggaga cacagcaugg     1860

gacuucggga gcgucggagg agcacugaac agccucggaa aaggaaucca ccaaaucuuc     1920

ggcgcagcgu ucaagagccu auucggcggg augagcuggu ucucacaaau acuaaucgga     1980

acacugcuca uguggcuagg ccuaaacacc aaaaacggau caaucagccu caugugucuc     2040

gcgcugggag gaguacugau cuuccuauca acagccguaa gugccgacgu cggcugc        2097


<210>  2792
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2792
aaaaaaagac ggggugccga caccucggua ggaauaguag gacuccuacu caccaccgcc       60

auggcagcag aaguaacacg ccgaggcagc gccuacuaua uguaccucga cagaaacgac      120

gccggcgaag ccaucuccuu ccccaccaca cucggcauga acaaauguua cauccaaaua      180

auggaucucg gccacaugug cgacgcaacc augucauacg agugcccaau gcucgacgaa      240

ggagucgaac ccgaugacgu agacugcugg ugcaauacca ccuccacaug ggucguauac      300

ggaacuugcc accauaaaaa aggagaagca agacgaucac ggagagccgu caccuuaccc      360

agccauucca cccggaaacu acaaaccaga agccaaacgu ggcucgagag ccgggaauau      420

acaaaacacc ucauaagagu cgaaaauugg auauucagaa accccggauu cgcccucgcc      480

gccgcagcca uagccuggcu acucggaucc agcacaagcc agaaagucau auaccucguc      540

augauacugc uaauugcccc agcuuauucc auccggugca uaggcgucag uaaccgggac      600

uucguagaag gaaugucagg agggaccugg guagacaucg ugcuagaaca cggaggcugc      660

guaaccguca uggcacaaga caaaccaaca gucgacauag aacugguaac cacuacaguc      720

agcaacaugg ccgaaguacg aucauacugc uaugaagcau caaucagcga cauggcaagc      780

gacagucgau gccccacgca gggcgaagcc uaccuagaca aacaaucaga cacacaauac      840

gugugcaaac ggacacuugu cgacagaggg uggggaaacg gcugcggccu auucgggaaa      900

ggcagccuag uaaccugcgc gaaauucgcg ugcucaaaaa aaaugaccgg caaaagcauc      960

cagccagaaa accuagaaua caggaucaug cucagcgucc acggcagcca gcacagcggu     1020

augauaguca acgacacugg ccacgaaaca gacgagaacc gcgcaaaagu ggaaaucaca     1080

ccaaacucac cacgcgccga agcaacccuc gguggguucg gcucccuugg acuagacugu     1140

gaaccaagaa ccggccucga cuucagcgac cuauacuacc ucacaaugaa caacaaacac     1200

uggcucgucc acaaagaaug guuccacgac auaccccuac cauggcacgc aggcgcagac     1260

acagggacuc cacacuggaa caauaaggaa gcgcuagucg aauucaaaga cgcccacgca     1320

aaaagacaaa ccguggucgu ccugggaucc caagaaggag cagugcacac cgcccucgca     1380

ggcgcucucg aagccgagau ggauggagca aaaggaagac uaagcagcgg gcaccucaaa     1440

ugccgacuaa aaauggacaa acuaagacua aaaggcguca gcuacagcuu auguacggcc     1500

gccuucaccu ucacgaaaau cccugccgaa acccuacacg gcaccguaac cgucgaaguc     1560

caauacgccg gaacagacgg gccguguaaa guccccgccc agauggcagu agauaugcaa     1620

acacucaccc ccguaggacg ccuaaucacg gcaaaccccg ucauaaccga aagcacagaa     1680

aacucaaaaa ugaugcucga acuagauccc cccuucggag acagcuacau aguaauagga     1740

guaggagaaa aaaaaaucac ccaccacugg cacagaucag gaaguaccau cggcaaggcc     1800

uucgaagcca ccgucagagg agccaaacga auggcggucc ucggagacac cgccugggac     1860

uucggaagcg ucggcggcgc acucaacuca cuaggcaaag gcauacacca aaucuucgga     1920

gccgccuuca aaagccucuu cggcggaaug agcugguucu cccaaauacu aaucggcacc     1980

cuacucaugu ggcucggucu caacaccaaa aacggcucca uaucccucau gugccuagca     2040

cucggaggcg uccucaucuu ccugagcacc gccgucagug ccgacguagg cugu           2094


<210>  2793
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2793
aagaggagag gcgcagauac gaguguaggu auaguagggc ucuugcucac gaccgcaaug       60

gcagccgaag ucacaagaag gggcucggca uacuacaugu accuggacag gaacgacgcc      120

ggcgaagcaa uaucguuccc aacaacgcua gggaugaaua aaugcuacau acagaucaug      180

gaucuagggc acauguguga cgccacaaug ucauacgaau gcccgaugcu ggaugaaggc      240

guagagcccg acgacgugga cugcuggugc aacacgacaa gcacaugggu agucuacggc      300

acaugccauc acaagaaagg cgaagcgaga agaaguagga gagcaguaac acugccaagc      360

cacucaaccc gcaagcuaca aacccgaagu cagaccuggc uagaaucaag agaguacaca      420

aaacaccuga uaagaguaga gaacuggaua uuccgaaacc caggauucgc cuuggcagcc      480

gcggcaauag ccuggcuauu aggcucgucc accagccaaa aggucaucua ucucgugaug      540

auacuacuaa ucgcccccgc auacucaauc aggugcauag gagucagcaa ccgcgacuuu      600

guagagggga ugucgggcgg gacaugggua gacaucguac uagaacacgg gggcuguguc      660

acagugaugg cccaagacaa acccacggua gauaucgaac ucgugaccac gacaguuagc      720

aacauggcag aaguuagauc guacugcuac gaagccagca uaagcgacau ggccucagac      780

ucgcgaugcc caacgcaagg ggaggcauac cuggacaaac aaucagacac ccaauacguc      840

ugcaaaagaa cccuagucga ccgaggcugg ggcaacgggu gcgggcuauu cggcaaaggc      900

agccugguaa caugugcuaa auucgcaugc agcaaaaaga ugacaggcaa aaguauacag      960

ccagagaacc uggaguacag gaucaugcua agcguccacg gcagccaaca cagcggcaug     1020

aucgucaacg acacaggcca cgaaacugac gaaaaccgag ccaaaguaga gauaacaccc     1080

aacagcccgc gcgcagaggc aacacugggc ggguucggga gccugggacu agacugcgaa     1140

ccgagaacgg gccuggacuu cagcgaccuc uacuacuuaa ccaugaacaa caaacacugg     1200

cuaguccaua aagagugguu ccacgacaua ccacuaccau ggcacgcagg cgcagacacc     1260

gggacaccac acuggaacaa caaagaagcc cuggucgagu ucaaggacgc acacgccaaa     1320

agacagacag ucgugguccu gggcucgcaa gaaggagccg uccacaccgc ccucgcggga     1380

gcacuagaag ccgagaugga cggggccaaa gggcgacuga gcucgggaca ucucaaaugc     1440

cgccuaaaga uggauaaacu ccgccucaaa ggcguaucau acagcuuaug caccgcggca     1500

uucacauuca caaagauccc cgccgagacc uugcacggga ccgugacagu agaagugcaa     1560

uacgccggaa cggauggacc augcaaaguc ccagcacaaa uggcagucga caugcaaacc     1620

cuaaccccag uaggacgccu aauaacggca aaccccguca ucacagaauc aaccgaaaac     1680

uccaaaauga ugcuggaacu agacccaccg uuuggcgaua gcuacaucgu aaucggagua     1740

ggagaaaaga aaaucacaca ucacuggcac cgaagcggca gcacaaucgg caaagcauuu     1800

gaagcaacag uacggggcgc aaaacgaaug gcaguacuug gagacaccgc augggauuuc     1860

gggagcguag gaggggcccu gaacucgcua gggaaaggaa uccaccaaau auucggggcc     1920

gcguucaaga gccuuuucgg aggaaugagc ugguucagcc aaauucucau agggacgcuc     1980

cugauguggc ucgggcuaaa caccaaaaac ggcucaauca gcuuaaugug ccuagcacua     2040

ggaggcgucc uaauauuccu aagcacagcc guaagcgcag acguaggcug c              2091


<210>  2794
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2794
cgccguggug cagacaccuc aguaggaauc guaggccuuc uccuaaccac agcuauggcc       60

gcagaaguca cacgacgggg aucggccuac uacauguacc ucgaccgcaa cgacgccggc      120

gaagcaauca gcuucccaac cacccugggc augaacaagu gcuacauaca aauaauggac      180

cucggccaca ugugcgacgc aacgauguca uaugaaugcc ccaugcucga cgaaggagua      240

gaacccgacg acguagacug cuggugcaac accaccagca ccugggucgu cuauggaacc      300

ugccaccaca agaaaggcga ggcccgcagg ucccgacgcg ccguaacgcu acccagccac      360

ucaacucgca aacuacaaac cagaagccaa accuggcuag aaucccgaga auacacgaaa      420

caccucauaa gagucgaaaa cuggauauuc cgcaacccag gcuucgcccu ggcagcagca      480

gcaaucgccu ggcuccuagg cagcagcacc ucccaaaaag uaauauaccu uguaaugaua      540

cugcucauag cacccgcaua cagcauacga ugcaucggag ucagcaacag agacuucguc      600

gaaggcauga gcggaggcac auggguagac aucguccuag aacacggagg gugcgucacg      660

guaauggcac aggacaaacc aacagucgac aucgaacucg uuaccacaac cguaagcaac      720

auggcagagg ucagauccua cugcuacgaa gcauccauaa gcgacauggc auccgacagc      780

cgaugcccaa cccaaggcga ggcuuaccua gacaaacaau ccgacaccca guacguaugc      840

aagcgaacuc uagucgauag gggauggggc aacggaugcg gacucuucgg caaaggcucc      900

uuagugacau gugcaaaauu cgccuguagc aagaaaauga ccggcaaaag cauccaacca      960

gaaaaccugg aguaccguau aaugcucagc guacacggaa gccaacacag cggcaugaua     1020

gucaacgaca caggacacga aacagacgaa aaccgugcca aaguagaaau caccccaaac     1080

agcccacgag cugaagccac ccuaggaggc uucggaagcc ugggacuaga cugcgaaccc     1140

cgcaccggcc ucgacuucuc cgacuuauac uaccugacaa ugaacaacaa acacuggcua     1200

guacacaaag agugguucca ugacauccca cuacccuggc acgcaggagc cgacaccggc     1260

acaccgcacu ggaauaauaa agaagcgcuc guagaguuca aggacgccca cgccaagcga     1320

caaaccgugg uaguccuggg cagccaagaa ggagcagucc acaccgcccu agcaggagca     1380

cuagaagccg aaauggacgg agcaaaaggg cgacucagcu caggacaucu caaaugccgc     1440

cugaaaaugg acaaacuccg gcucaaggga gucagcuaca gccugugcac cgcagccuuc     1500

accuucacca aaauacccgc agaaacccuc cacggcaccg uaacaguaga aguccaauac     1560

gcaggaaccg acggacccug caaaguacca gcccaaaugg caguagacau gcaaacacuc     1620

acacccgucg guagauuaau aacagccaac ccagucauaa ccgaaucgac ugaaaacagu     1680

aaaaugaugc ucgagcuaga ucccccauuc ggggacagcu acauaguaau cggagucggc     1740

gagaaaaaga ucacacauca cuggcaccgc agcggcagca ccaucggcaa ggcuuucgaa     1800

gcaacaguac gcggggcaaa acggauggcc guccuaggcg acacagccug ggacuucggc     1860

uccguaggcg gggcacucaa cagccuaggg aaaggcaucc accaaaucuu cggagccgcc     1920

uucaaaucgc uguucggagg aaugagcugg uuuagucaaa uacuuauagg cacccuacuc     1980

auguggcucg gacuaaauac caaaaacggu uccaucagcc ucaugugccu agcccuaggc     2040

gggguacuga ucuuccucuc aaccgccguc uccgccgacg uaggcugu                  2088


<210>  2795
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2795
agaggagcug acacgagcgu cggcauaguc ggacuccucc uaaccacagc cauggcagcc       60

gaaguaacac gacguggauc cgccuacuac auguaccugg acagaaacga ugcaggcgaa      120

gcuaucagcu uccccaccac ccucggcaug aacaagugcu acauacagau cauggaccua      180

ggccacaugu gcgacgcgac gaugagcuac gaaugcccga ugcucgacga aggcguggaa      240

cccgacgacg ucgacugcug gugcaacaca accagcaccu ggguugucua cggcacgugc      300

caccacaaga aaggagaagc acgccgaucc cgcagagccg uaacccuacc cucacauucc      360

acaagaaaac uacaaacccg aagccaaaca uggcuagaaa gccgcgagua cacaaaacac      420

cuaauaagag uagaaaauug gauauuccga aaccccgguu ucgcccucgc agccgccgcg      480

aucgccuggc uacuaggauc cuccacuagc caaaaaguaa ucuaccucgu caugauacua      540

cucauagccc cagccuacuc cauccgcugc aucggagugu caaacaggga cuucguagaa      600

ggcaugagcg gaggcacgug ggucgacauc guacuagaac acggcgggug cgucacagug      660

auggcccagg acaaaccaac cguagacauc gagcuaguca cgaccacagu aagcaacaug      720

gcugaaguca gaucguacug cuacgaagcc uccaucuccg acauggcauc cgacucccgc      780

ugcccaaccc aaggcgaagc auaccucgac aaacaaucgg acacccaaua uguaugcaaa      840

cgcacacucg uagacagagg auggggcaac ggcugcggac uauucggaaa agggagccua      900

gucacuugug ccaaauucgc cugcagcaag aaaaugaccg gaaaaaguau ccaacccgaa      960

aaccuagaau accgcaucau gcucuccguc cauggauccc aacacagcgg caugaucgua     1020

aacgacaccg gccacgaaac agacgaaaac cgcgccaaag ucgaaaucac ccccaacucc     1080

ccaagagcag aggccacccu gggaggauuc ggaucacuag gccucgauug cgaaccgcgg     1140

accggccuag acuucucaga ccuguauuac cuuaccauga acaacaagca cuggcucgua     1200

cacaaggaau gguuccacga uauaccacua cccuggcacg ccggcgccga cacaggcaca     1260

ccccacugga acaacaaaga ggccuuaguc gaguucaaag acgcccacgc aaagcgucaa     1320

accguagucg uacuuggauc gcaagaaggc gccguccaca ccgcccucgc aggggcccuc     1380

gaagcagaaa uggacggcgc gaaagggcgc cuauccagcg gacaccucaa augcagacua     1440

aaaauggaua aacuccgccu gaaaggcguc ucauacuccc ucugcaccgc cgccuucacc     1500

uucacaaaaa uccccgcaga aacccuccac gggacaguaa ccgucgaagu ccaguacgcc     1560

ggaacagacg gacccugcaa aguaccugcg caaauggcag uagacaugca aacccuaacc     1620

cccgugggac gacuaauaac ggcaaaccca gucauaaccg aauccacaga aaacucaaaa     1680

augaugcucg agcucgaccc acccuucggc gacagcuaca ucgucaucgg agucggagag     1740

aaaaagauaa cacaccacug gcacagaucc ggcagcacga ucggcaaagc auucgaagcc     1800

accguacgug gagcuaaacg cauggcaguc cucggagaua cagccuggga cuucggcucc     1860

gucggaggcg cccucaacag ccuaggcaaa ggaauacacc aaauauucgg ugcagcauuc     1920

aagucccucu ucggcggcau gagcugguuc ucacagaucc ucauugggac ccuacucaug     1980

uggcuagguc uaaacaccaa aaacggcucc aucucccuca ugugccuggc acuagguggc     2040

guacucaucu uccucucgac cgcgguuuca gccgacgucg ggugc                     2085


<210>  2796
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2796
ggcgcggaca caagcgucgg gaucguaggc cuucugcuca cgaccgccau ggcagccgaa       60

gucacgcgac ggggaucggc cuacuacaug uaccuugaca gaaacgacgc cggugaagca      120

auauccuucc cuaccacccu cggaaugaac aaauguuaua uacagaucau ggaccucggc      180

cacaugugcg acgcaaccau gucauacgaa ugccccaugc uagacgaagg aguggagcca      240

gacgacgugg acuguuggug caacacaacg agcacauggg uaguuuacgg aacaugccau      300

cacaagaaag gagaagcuag aagauccaga cgcgcaguca cacuaccauc ccacagcacg      360

cgcaaacucc agacacgcag ccaaacaugg cuggagucaa gggaauacac aaaacaccuc      420

auacgagucg agaacuggau cuuccgcaac cccggauucg cacucgcagc cgcagcaaua      480

gcauggcuuc ucgggagcuc caccagccaa aaaguaaucu accucguaau gauacuccua      540

aucgcaccag cguacuccau aagaugcaua ggcguaagca accgagacuu cgucgaagga      600

augagcggug gaacgugggu ugacaucgua cuggaacacg gggguugugu cacggucaug      660

gcacaagaca aaccaacggu ggacaucgag cuaguaacaa cgaccgucag caacauggca      720

gaaguccgau ccuacugcua cgaagcauca aucagcgaca uggccucaga cucccgaugc      780

cccacccaag gagaagccua ccuagacaag caauccgaca cucaauacgu cugcaaaaga      840

acgcuagucg accguggaug gggaaacggc ugcgggcuau uuggaaaagg aucucuaguc      900

acuugcgcaa aauucgcgug uagcaagaaa augacaggaa aaagcauaca accggaaaau      960

uuagaauaca ggauaaugcu cuccguccac ggcucccaac auagcggcau gaucgucaac     1020

gacacagguc acgaaacaga cgaaaacagg gccaaaguag aaaucacccc caacucaccg     1080

cgcgcagaag caacacuggg cggcuucggc ucacuaggcc ucgacugcga accacgcacu     1140

ggcuuagacu ucagcgaccu cuacuaccug acaaugaaca auaaacacug gcuaguccac     1200

aaagaauggu uccacgacau accgcuacca uggcaugcag gagcggacac aggcacacca     1260

cacuggaaca acaaagaagc ccucgucgag uucaaagacg cccacgcaaa aagacaaacg     1320

gucguagucc uaggcuccca agagggggca guacacacag cacucgcagg agcacucgaa     1380

gccgagaugg acggagcgaa aggccgacua ucaucgggac accucaaaug caggcucaaa     1440

auggacaaac ugcggcuaaa aggagucucc uacucacucu gcacagcggc cuucacuuuc     1500

accaaaaucc cagccgaaac ccuacacggc accgucaccg uggaaguaca auacgccggc     1560

accgacggcc cgugcaaggu accagcccaa auggccguag acaugcagac cuugacccca     1620

guaggaagac uaaucacagc caacccgguc auaacagaaa gcaccgagaa cuccaaaaug     1680

augcuggaac uggacccacc cuucggagac agcuauaucg ucauaggagu uggagagaaa     1740

aagaucaccc accacuggca ccgaagcgga ucaaccaucg gcaaagccuu cgaagccaca     1800

guccgaggcg caaaaagaau ggccguccua ggcgacacgg ccugggacuu cgggagugua     1860

gguggagcac ucaacucacu cggaaaaggc auccaccaaa uauucggggc cgccuucaag     1920

agccuauucg gaggcaugag cugguucucg caaauacuaa ucggcacacu gcucaugugg     1980

uuaggccuua acacuaaaaa cggcaguauc agccuaaugu gccucgcacu cggaggcgug     2040

cucauauucc ugagcacagc agugagcgcg gacgugggau gc                        2082


<210>  2797
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2797
gcagacacca gcgucggcau aguagggcua cuacucacaa cagcaauggc agccgaggua       60

acacggcgcg gcucagccua uuacauguac cucgacagaa acgacgcagg agaagcaauc      120

agcuucccca ccacgcuagg caugaacaaa ugcuacauac aaauaaugga ccuaggccac      180

augugcgacg cuacaaugag uuacgaaugc ccaaugcugg acgaaggcgu agaaccagac      240

gacgucgacu gcuggugcaa caccacuagc accugggucg ucuacggaac cugccaccau      300

aagaaagggg aagcgcgccg cagccggcga gcgguaacac uccccagcca cagcacgcgc      360

aaacuccaga cccgcagcca gacauggcua gaauccagag aauacaccaa acaccuaaua      420

agaguagaga acuggaucuu ccgcaaccca ggauucgccc ucgccgccgc agccauagcc      480

ugguuauuag gcuccuccac aagccagaaa guaaucuacc ucguaaugau acuccuaauc      540

gcacccgcau auuccauaag augcauaggc guaagcaacc gcgacuucgu ugaagggaug      600

ucgggcggaa ccugggucga caucgugcuc gaacacggag gaugcgucac ggucauggcc      660

caagacaaac ccaccgucga caucgaguua gucacuacaa caguguccaa cauggccgag      720

guacgcuccu acugcuacga agcaagcaua uccgacaugg ccuccgacag ucgcugccca      780

acacagggag aagcauaccu cgacaaacag agcgacacac aauacguaug caaacgaacc      840

cuaguagacc gaggaugggg caacggaugc ggacuauucg gcaaaggcuc ccuagugacc      900

ugcgcaaaau ucgcaugcuc aaagaaaaug acgggaaaaa gcauacaacc agaaaaccuc      960

gaguaccgaa uaaugcuauc gguccacggc agccaacaca gcggaaugau aguaaacgau     1020

accggacacg aaacagacga aaacagagcc aaaguagaga uaacucccaa cagcccacga     1080

gcugaagcga cucugggagg auucggcagc cugggccucg acugugaacc gcgcaccggc     1140

cuagacuucu cagaucuuua uuaccucacu augaauaaca aacauuggcu cguacacaaa     1200

gaaugguucc augacauacc acuaccaugg cacgccggag cagacaccgg caccccacac     1260

uggaacaaca aagaggcccu cguagaguuc aaagacgccc acgcgaaacg acaaacaguc     1320

gugguucuag gcucacaaga aggagcgguc cacaccgcgc uagcuggcgc acucgaagca     1380

gaaauggacg gagcaaaagg acggcucagc uccggccacc ucaaaugccg acuaaaaaug     1440

gacaagcucc ggcugaaagg agucagcuac ucccucugua ccgccgccuu caccuucaca     1500

aaaaucccag cagaaacacu acacggcaca guaacaguag agguccaaua cgcggggacc     1560

gacgguccau gcaagguacc agcccaaaug gcaguagaca ugcagacccu aacgcccgua     1620

ggacgccuga uaaccgcaaa cccaguaaua accgaaagca ccgaaaacag caaaaugaug     1680

cuggaauuag acccgccauu cggcgacagc uauaucguca uuggcgucgg cgaaaagaaa     1740

aucacccacc acuggcaccg caguggaucc acaauaggaa aagcauucga ggcaaccgua     1800

cgaggcgcaa aacgaauggc cguccucgga gauacagcgu gggacuucgg cuccgucggc     1860

ggagcacuca acagccuagg gaaagguaua caccaaauau ucggcgcagc uuucaaaagc     1920

cuauucggag gaaugagcug guucagccaa auacuaaucg gcacacuccu cauguggcuc     1980

ggacucaaca ccaaaaacgg gagcauaagc cucaugugcc uggcgcucgg aggcguacuc     2040

aucuuccucu caacggcagu aagcgcagac gugggaugc                            2079


<210>  2798
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2798
gacaccuccg ucggcauagu gggacuccuc cuuacuaccg ccauggcagc ggaaguaacc       60

cgacgcggcu cagcauacua cauguaccug gaccgcaacg acgcaggcga ggccaucagc      120

uuucccacca cccugggaau gaacaagugu uacauacaaa ucauggaccu cggccacaug      180

ugcgacgcaa ccaugagcua cgaaugccca augcucgacg aaggaguaga acccgacgac      240

guagacugcu ggugcaacac aaccucgaca ugggucguau acggaaccug ccaccacaag      300

aaaggcgagg cccgccguuc acgccgagcc gucacacuac caucccacuc cacaagaaaa      360

uuacaaacgc gcucacaaac auggcucgag uccagagaau acaccaaaca ccuaauccga      420

guagaaaacu ggaucuuccg aaaccccggc uucgcccucg ccgcagccgc aauagcgugg      480

cuccucgguu ccagcaccuc ccaaaaaguc aucuaccuag uaaugauacu acuaaucgcu      540

ccagcuuacu ccauaagaug cauaggcgua uccaaccggg acuucgucga aggcaugagu      600

ggcggcacau gggucgacau cguccuagag cacgggggcu gcguaacagu cauggcccaa      660

gacaaaccaa cggucgacau agaacucguc accaccacag ucucaaacau ggcagaaguc      720

cgcuccuacu gcuacgaagc gagcaucagu gacauggcau cagacagccg cugcccaacc      780

caaggagaag ccuaccuaga caaacaaucc gauacucaau acgucugcaa aagaacauua      840

guggacagag gcuggggaaa cggcugcggc cucuuuggaa aaggcagccu aguaacaugc      900

gccaaauucg cuugcucuaa gaaaaugacc ggcaaaucaa uccaacccga aaaccuggag      960

uaucgcauca ugcuaagcgu acacggcagc caacacagcg gaaugauagu uaaugacacc     1020

ggccacgaaa cagacgaaaa ccgcgcaaaa gucgaaauca cccccaacuc accccgcgca     1080

gaagccaccc uaggcgguuu cggcucacuc ggcuuagacu gcgaaccacg uacaggacua     1140

gacuucucag accuguacua ccucaccaug aauaacaagc acuggcucgu ccacaaagaa     1200

ugguuccacg acauaccccu gccauggcac gcgggagcgg acaccggcac accccacugg     1260

aacaacaaag aagcccuagu ggaguucaaa gacgcccacg caaaacgcca aaccguaguc     1320

guucucggaa gucaggaagg ggccguccac acagcccuag ccggagcccu cgaagcagaa     1380

auggacggug cgaaagggcg acucuccucu ggacaccuca aaugccgcuu aaaaauggac     1440

aaacuacggc ucaaaggagu cuccuacagc cucugcacgg ccgccuucac cuucacaaaa     1500

auccccgccg aaacacucca cggaacaguc acaguagaag uccaauacgc cggaaccgac     1560

ggccccugca aagucccagc acaaauggca gucgacaugc aaacacucac acccguaggc     1620

agacucauca ccgccaaccc agucauaaca gaaucaacag aaaauuccaa gaugaugcug     1680

gaacucgauc ccccauucgg cgacucauau aucgucauag gagugggaga aaagaaaauc     1740

acacaccacu ggcaucgcuc aggaagcacc aucggcaaag cauucgaagc uaccguccga     1800

ggagcgaaac ggauggccgu acuaggcgac acggccuggg acuucggcuc cguaggcgga     1860

gcccucaauu cccuagggaa aggcauccac caaaucuucg gcgccgccuu caaaucccua     1920

uucggcggca uguccugguu cagccaaaua cuaaucggaa cacuacucau guggcuagga     1980

cucaacacca aaaacggcuc caucagccuc augugccuag cucugggcgg aguccucauc     2040

uuccucagca ccgcuguauc cgccgacguc ggcugc                               2076


<210>  2799
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2799
accagcgucg guaucguagg ccuacuacua acgaccgcaa uggcugccga aguaacaaga       60

cgcggcagcg cguacuacau guaccucgac cgaaacgacg caggcgaagc aaucagcuuc      120

ccgaccacac uaggcaugaa caaaugcuac auacaaauaa uggaccucgg ucauaugugc      180

gacgcaacaa ugaguuacga augcccaaug cucgacgaag gaguagagcc cgacgacguc      240

gacugcuggu gcaacaccac cagcacaugg gucgucuacg gaacgugcca ccacaagaaa      300

ggcgaagccc gcagaucccg ccgcgcaguc acccucccca gccacucaac gagaaaacuc      360

cagacccggu cacaaacgug gcucgaaucc cgagaguaca caaaacaccu cauacgcgug      420

gaaaauugga ucuuccgcaa ccccggcuuc gcacucgccg cagccgccau agccuggcua      480

cucggauccu caacauccca aaaagucaua uacuuaguaa ugauccuacu cauagcacca      540

gcuuacagca uccgcugcau aggcguaucc aacagagacu ucgucgaagg aaugagcggc      600

ggaaccuggg uagacauagu ccuggaacac gggggaugcg uaaccgugau ggcacaggac      660

aaacccacag ucgacauaga acucgugaca acaacgguaa guaacauggc ggaaguucgc      720

agcuacugcu acgaagccuc aaucuccgac auggcaagcg acucacggug cccaacgcag      780

ggcgaagccu accuagacaa acagucagau acacaauacg ucugcaaacg aacccucgua      840

gaccggggcu ggggcaacgg augcggccuc uucggaaaag gaucacucgu cacaugcgca      900

aaguucgcau gcaguaagaa gaugacgggc aaaagcaucc agccagagaa ccucgaauac      960

cgcauuaugc ucucagucca cggaucccaa cacucaggca ugaucgugaa cgacacagga     1020

cacgaaacag acgaaaaccg agcgaaagua gaaauaaccc caaacagccc cagagcagaa     1080

gcaacccucg gcggcuucgg cagucucgga cuagacugcg aaccaagaac aggccucgac     1140

uucagcgauc uguacuaccu aacgaugaac aacaaacacu ggcucguaca caaagaaugg     1200

uuccacgaca uaccacuccc guggcacgca ggagcagaca cgggcacacc ccauuggaac     1260

aacaaagagg cccuagucga guucaaggac gcccacgcca aacggcagac cgucguggua     1320

cuaggcucac aagaaggagc agugcacaca gcacuagcag gagcacuuga ggcagaaaug     1380

gacggcgcca aaggucgacu gucaagcggg caccuaaaau gucgccuaaa aauggacaaa     1440

cuacgccuca aaggcguaag cuauagccuc ugcaccgcag cguucacguu cacgaaaaua     1500

ccggcagaga cccuacacgg cacaguaacg guugaagucc aauacgccgg caccgacggg     1560

ccaugcaaag ucccggccca aauggccguc gacaugcaaa cgcuaacacc ggucggacgc     1620

cucaucacag ccaacccggu caucacagaa agcacagaga acaguaaaau gaugcuagaa     1680

cuagacccac ccuucggcga cucauacauc guaauaggcg uaggagagaa gaaaauaacc     1740

caccacuggc acagaagcgg caguaccaua ggcaaagcau ucgaagcaac cguaagaggc     1800

gccaaacgca uggcaguacu aggcgacacc gccugggacu ucggcagcgu aggaggagcc     1860

cuaaacuccc ucggcaaagg cauacaccaa auauucggcg cagccuucaa aagccucuuc     1920

ggcgggauga gcugguucuc ccagauacua auaggcaccc uacuuaugug gcuaggacua     1980

aacaccaaaa acggaagcau cagccucaug ugccucgcac ucggcggagu acuaaucuuc     2040

cucuccacag cggucucagc agacgucggc ugu                                  2073


<210>  2800
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2800
accucaguug gaaucgucgg ccuacuucug acaaccgcca uggcagccgc cgucacacgc       60

aggggcucag cauauuacau guaccuggac cgaaacgacg ccggggaggc caucuccuuc      120

cccaccaccc ucgggaugaa caaaugcuac auccaaauaa uggaccuggg ccacaugugc      180

gacgcgacca uguccuacga gugccccaug cucgacgagg gaguagaacc cgacgauguc      240

gacugcuggu gcaacaccac gagcaccugg guggucuacg gaacgugcca ccacaagaaa      300

ggcgaagccc gacgcucccg cagggcggug acccuaccca gccacagcac acggaaacua      360

caaaccaggu cccagacaug gcucgagucc agagaauaca ccaaacaccu aauucgcguc      420

gaaaacugga ucuucagaaa cccaggauuc gcccuagccg cagccgccau cgcauggcuc      480

cucggcucaa gcacaagcca aaaagucauc uaccuaguca ugauacugcu aaucgccccc      540

gccuacucaa uccgcugcau cggaguauca aaucgcgauu ucguugaggg aaugagcgga      600

ggcaccuggg ucgacaucgu ccucgaacac ggcggcugcg uaacagucau ggcucaagac      660

aaaccaacag uagacaucga gcuaguaaca accaccguaa gcaacauggc agaaguacgg      720

ucauacugcu acgaagcauc uauaucagac auggccucag acucacgaug cccaacucaa      780

ggggaagcau aucucgacaa acaaagcgac acccaauaug uaugcaaacg aacacucguu      840

gacagaggcu ggggaaacgg cuguggccuc uucggcaaag gcucccuugu cacaugcgca      900

aaauucgccu gcucgaagaa aaugaccggc aaaagcauac aaccagaaaa ccuggaauac      960

agaauaaugc ugagcguaca cggcucgcaa cauagcggca ugauaguaaa ugacaccgga     1020

cacgaaaccg acgaaaaccg agccaaagua gaaaucaccc ccaauucucc ccgcgccgaa     1080

gccacucugg gaggcuucgg aucccuaggc cucgacugcg aaccaagaac cggacuagac     1140

uucucagacc uauacuaccu aacaaugaac aacaaacacu ggcuugucca caaagaaugg     1200

uuccacgaca ucccccuacc cuggcacgca ggcgccgaca caggaacgcc ccacuggaac     1260

aacaaagaag cacucguaga guucaaagac gcccacgcga aacgccaaac cguagucguc     1320

cugggcucac aagaaggagc aguccacacc gcgcucgccg gugcacucga agcagaaaug     1380

gacggcgcca aaggccgacu aagcuccggc caucuaaaau gccgucuaaa aauggacaaa     1440

cuccgccuaa aaggagucag cuacucacug uguacagcag ccuucaccuu caccaaaauc     1500

ccggccgaga cgcuacacgg caccgucacc gucgaaguuc aguacgcagg gaccgacgga     1560

cccugcaaag uccccgccca aauggcggua gacaugcaaa cccugacccc ggucggaaga     1620

cuuauaaccg caaaccccgu aaucaccgaa ucaaccgaga acagcaaaau gaugcuggag     1680

cuagacccac ccuucggcga cuccuacauc guaaucggcg ucggcgaaaa gaaaauaacc     1740

caccacuggc accgaucggg aucaaccaua ggaaaagcgu ucgaagcaac cguccgcgga     1800

gcaaaacgga uggccgugcu aggagacaca gcaugggacu ucggaagcgu aggaggcgcc     1860

uuaaacucac uaggcaaagg cauacaccaa aucuucggcg ccgccuucaa gagcuuauuc     1920

ggaggcauga gcugguucuc ccaaauccua aucggcacac uccucaugug gcugggacuc     1980

aacaccaaaa acgggagcau aucgcucaug ugccucgccc uggguggcgu ccuaaucuuc     2040

cuaucgacag ccgucuccgc cgacgucggc ugc                                  2073


<210>  2801
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2801
cgcggcgccg acaccagcgu aggcaucguc ggacuacucc ucaccacagc cauggcagcc       60

gcaguaacac gacgaggauc agccuacuac auguaccugg acagaaauga cgccggcgaa      120

gccaucagcu uuccaacaac ucucgguaug aacaagugcu acauacaaau aauggaccuc      180

ggccacaugu gcgacgcaac aaugagcuac gaaugcccca ugcuagacga aggggucgag      240

cccgacgacg uagacugcug gugcaacacc accucaaccu gggugguaua cggaacaugc      300

caccacaaga aaggcgaagc ucgacgcagc agacgagccg uaacacuccc aagccacagc      360

acaagaaaac uccaaaccag aagccaaaca uggcucgaau ccagagaaua cacaaaacac      420

cuaauuagag uagaaaacug gauauucaga aaccccgguu ucgcacucgc cgccgccgca      480

auagcauggc uacucggaag cuccaccucg caaaaaguga ucuaccucgu caugauccua      540

cuaaucgcac cagccuacuc caucagaugc aucggagucu ccaaccgaga cuucgucgaa      600

ggaaugagcg gcggaaccug gguagacauc gugcuagaac acggcggaug cgucacugua      660

auggcacaag acaaaccaac agucgacauc gaacucguaa ccacaacggu cuccaacaug      720

gccgaagucc gauccuacug uuacgaggcc agcauaucag auauggcauc cgacagccga      780

ugccccaccc aaggcgaagc cuaccuagac aaacaaagcg acacccaaua cguaugcaaa      840

cgcacccucg uugacagagg cuggggaaac ggaugcgggc uauucggcaa gggcucacuc      900

gucacaugcg ccaaauucgc cugcagcaaa aagaugaccg ggaaaagcau acaacccgaa      960

aaccuagaau accgaaucau gcuaucagug cacgggucac agcauagcgg caugaucguc     1020

aacgacaccg gacacgagac ggacgaaaac agggccaaag ucgagaucac ccccaacuca     1080

ccgcgcgcag aagccacgcu cggcggauuc ggcucauuag gacuggauug cgaaccacgu     1140

accggccucg acuucuccga ccucuacuac cucacaauga auaacaaaca cuggcuggua     1200

cacaaagaau gguuccacga cauaccccua cccuggcacg ccggagccga cacaggcaca     1260

ccgcacugga acaacaagga agcccucguc gaguucaaag acgcccacgc uaaaagacaa     1320

accgucgucg uacuaggaag ccaagaaggc gcagugcaca ccgcgcuggc gggcgcacuu     1380

gaagccgaaa uggauggagc aaaaggaaga cuaagcuccg gacaccugaa augucgccua     1440

aagauggaca aacuaaggcu aaaaggcgua ucguacucac ucugcacagc agcauucacu     1500

uucacaaaaa uacccgccga aacgcuccac ggcaccguaa cagucgaagu ccaauacgcg     1560

ggaaccgacg gcccaugcaa aguccccgcc caaauggccg uggacaugca gacccuaacc     1620

ccggugggcc gccuaauaac agcaaacccc guaaucacug aaagcaccga aaauucaaaa     1680

augaugcucg aacucgaccc acccuucgga gacagcuaca uagugauagg aguaggagag     1740

aaaaagauua cccaccauug gcacagauca ggcagcacaa ucggaaaggc uuucgaagca     1800

accguacgcg gagcaaaacg cauggcggua cucggcgaca cagccuggga cuucggcagc     1860

gucggcggcg cacuaaacuc cuuaggaaag gguauccacc aaaucuuugg cgcagccuuc     1920

aaaagccucu ucggcggcau gagcugguuc ucacaaaucc ucauaggcac acuccucaug     1980

uggcucggcu uaaacaccaa aaacggaucc aucagccuaa ugugccuggc acucggcggc     2040

guccuaaucu uucuaucaac ugccgucagu gcagacguag gaugc                     2085


<210>  2802
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2802
cgaggccuca gcuccagaaa acgacgauca cacgacgucc ucacgguaca auuccucaua       60

cuagguaugc uacucaugac cggaggagug acacgcagag gcagcgccua cuacauguac      120

cucgaccgca acgacgcagg agaggccauc agcuucccca caacccuagg caugaauaaa      180

ugcuacaucc aaaucaugga ccuaggccac augugcgacg ccacaauguc auacgaaugc      240

cccaugcuug acgaaggagu agaacccgac gacguagacu gcuggugcaa cacaaccagc      300

accugggucg uauacggaac gugccaccac aagaaaggug aagcacgaag aagccgaagg      360

gcagucaccc uucccagcca cucaaccaga aaacuccaaa cacgauccca aaccuggcua      420

gagagccgcg aguauacgaa acaccucauc cgcgucgaaa acuggaucuu cagaaaccca      480

ggcuucgcgc ucgcugcagc agcaauagca uggcuccugg gaagcagcac cucgcaaaag      540

guuauauacc uagucaugau acuacugaua gcacccgccu acagcauacg cuguaucgga      600

guauccaaca gagacuucgu cgaaggaaug uccggaggaa cauggguaga uauagugcuc      660

gaacacggug ggugcguaac cgucauggcc caggacaaac ccaccgucga cauagaacuc      720

gucacaacca cgguaucaaa cauggccgaa guacgaucau acugcuacga agcaagcauu      780

agcgacaugg cguccgacuc aaggugccca acccaaggag aagcauaccu agacaaacaa      840

agcgacacgc aauacgugug caagcgaacc cuuguagaca gaggaugggg gaacggaugc      900

ggccucuucg gaaaaggcag ccuaguaaca ugcgcaaaau ucgcaugcag caagaaaaug      960

accggaaaau cgauacaacc agagaaccuc gaauacagaa uaaugcucag cguccacggu     1020

ucacagcaca gcggcaugau agugaacgac acagggcacg agaccgacga aaaucgcgcu     1080

aaagucgaga uaaccccaaa cagcccacga gcagaagcca cccucggggg cuucggauca     1140

cuuggacuag acugcgaacc acgaaccgga cuagauuuca gcgaccucua cuaccuaaca     1200

augaacaaca aacacuggcu cguacacaaa gaaugguucc acgauauccc guuaccaugg     1260

cacgcaggag ccgacacagg aacuccccac uggaacaaca aagaagcacu cguggaguuc     1320

aaggacgcuc acgcaaaacg ccaaaccgug gugguccuag gaagccagga aggagccguu     1380

cacacagccu uagccggagc acuagaagcg gagauggacg gggccaaggg ccgccugagc     1440

aguggacacc ucaagugccg acugaaaaug gacaaacuaa gacuaaaagg agucucguac     1500

agccuaugca cagcagccuu caccuucaca aagauaccgg ccgaaacacu acacggcaca     1560

gugacaguag aaguacaaua cgcaggaacc gacggccccu gcaaaguacc cgcccaaaug     1620

gcaguagaca ugcaaacccu cacacccgua ggcaggcuaa ucacggcgaa cccaguuaua     1680

acggaaagca cggagaacag caaaaugaug cuagagcugg acccaccauu uggagacucg     1740

uacauaguaa ucggagucgg agaaaagaaa aucacacacc acuggcaccg uuccggcagc     1800

acgauaggca aggcguucga agcaacaguc agaggcgcca agaggauggc gguacucgga     1860

gacaccgccu gggacuucgg cucagugggc ggcgcgcuaa acucacucgg aaaaggcaua     1920

caccaaauau ucggagccgc auucaaaagu cuuuucggag gcaugagcug guucagccaa     1980

auccugauag gcaccuuacu aauguggcuc ggcuuaaaca ccaaaaacgg aaguaucuca     2040

cucaugugcu uagcccuggg cggaguucuc auauuccuua gcaccgcagu cagcgccgac     2100

guugggugc                                                             2109


<210>  2803
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2803
cgcggccucu cuagccgcaa acgcagaagc cacgacgucu uaacuguaca auucuuaauc       60

cucggcaugc ugcuaaugac aggggcagua acacgccgag gauccgcuua cuauauguac      120

cuggaccgga acgacgcagg cgaagccaua uccuucccaa caacccuggg caugaacaaa      180

ugcuacauac aaauaaugga ccuaggucac augugcgacg cgaccaugag cuacgagugc      240

cccaugcucg augaaggagu cgaacccgac gacgucgacu gcuggugcaa caccacgucu      300

accuggguag ucuacggaac cugccaccac aaaaagggag aagcacggcg aucuaggcga      360

gcaguaacgc uaccaucaca uagcacgcgg aaacuccaga cacgauccca aaccugguua      420

gaaagccgag aguacaccaa acaccugaua agaguagaaa acuggaucuu cagaaaccca      480

ggauucgcuc uagcggcagc agccaucgcc uggcuccuug gaucaucaac aucucaaaaa      540

guaaucuacc ucguaaugau ccuccucauc gcaccggcau acagcauacg cugcaucggc      600

gucucaaaca gagacuucgu agaagggaug agcggcggca cauggguaga cauaguacuc      660

gaacacggcg gcugcguaac cguaauggcg caggacaaac caaccgucga caucgagcua      720

gucacgacca ccgucucaaa cauggcugaa guccgaucau acugcuacga agcaagcauc      780

agcgauaugg ccuccgacag uagaugcccc acccaaggag aagccuaccu ugacaaacaa      840

uccgacaccc aauacguaug caagcgaacc cucgucgacc gcggcugggg caacggcugc      900

ggccuauucg gcaaaggcag ccucgucaca ugcgccaaau ucgccugcag caagaaaaug      960

accggaaaau caauacaacc agaaaaccuc gaauaccgaa ucaugcucag cguccacggc     1020

agccaacacu ccggcaugau agucaacgac acaggccacg aaacagacga gaaccgugcc     1080

aaaguagaga uaacacccaa caguccccgu gccgaagcaa cccucggcgg cuucggcucc     1140

cuaggccugg acugcgaacc acgaaccgga cuagacuuca gcgaccuaua cuaccuaaca     1200

augaacaaca agcacuggcu gguacacaaa gaaugguucc acgacauccc ccuccccugg     1260

cacgcgggcg ccgacacggg aaccccacac uggaacaaca aagaagcccu cgucgaguuc     1320

aaagacgcgc acgcaaaaag acaaacaguc gucguauuag gcucacaaga aggcgcagua     1380

cacacagccc uagcuggagc ccucgaagca gaaauggacg gggcaaaagg ccgacucagc     1440

agcggacacc ucaaaugccg ccuuaaaaug gacaaacuac gacuaaaggg agucagcuac     1500

ucacucugca ccgccgcauu cacguucacc aaaauccccg ccgaaacccu ccacggaacc     1560

guaacaguag aaguacaaua cgcaggcacc gacggucccu gcaaaguccc agcccaaaug     1620

gcaguagaca ugcagacacu aacgccugua gggagacuaa uuacagcaaa cccugucauc     1680

acggaaucaa ccgagaacag caaaaugaug cuagaacuag aucccccauu cggagacagu     1740

uacaucguaa uuggagucgg agaaaagaag auaacgcacc acuggcacag aagcggcuca     1800

acaauaggaa aagcauucga agccacaguc cggggagcca agagaauggc cguacugggg     1860

gacaccgcau gggacuucgg aagcgugggc ggagcacuaa acagccuagg caaagggaua     1920

caccaaauau ucggcgccgc cuucaaauca cucuucggag gcaugagcug guucagccag     1980

auucuuaucg gcacacuacu aauguggcuc ggccugaaca ccaaaaacgg uagcauauca     2040

cucaugugcc uugcccucgg agguguacua aucuuccuga gcaccgccgu cagcgcagac     2100

guaggaugc                                                             2109


<210>  2804
<211>  2109
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2804
cgaauaauaa acgcacgaaa agagaagaaa agaagaggcg ccgacacaag cgugggaaua       60

gucggccuac ugcuaacaac agccauggcc gccgaaguca cgcgucgagg auccgccuac      120

uacauguacc ucgaccgaaa cgacgccggc gaagccauau ccuucccaac aacccucggc      180

augaacaaau gcuacaucca aauaauggac cugggacaca ugugcgacgc caccauguca      240

uacgaaugcc caaugcuaga cgaaggcguc gaaccagacg augucgacug cuggugcaac      300

acaacuagca cauggguagu auacggcaca ugccaccaca agaaagggga agcccgccgc      360

agccggaggg ccgucaccuu accaucccac ucaacgcgca aacuccaaac ccgaagccaa      420

acauggcucg aaucgcgaga auacaccaaa caccucaucc gggucgagaa cuggaucuuu      480

cgaaaccccg gguucgcccu agcggcggcg gcaauagcau ggcuacucgg cucaucaacg      540

ucucaaaaag ucauuuaucu agucaugaua cugcuaaucg ccccagcgua cucaauacgg      600

ugcaucggag uaucaaaccg cgauuucgug gagggcaugu caggcggaac cuggguagac      660

auagugcuag agcacggagg cuguguuacu gucauggccc aggacaaacc caccguggac      720

aucgagcuag uaacaacaac cguauccaac auggcagaag uccguucaua cugcuaugaa      780

gccuccaucu ccgacauggc aucagauagc agaugcccaa cacagggcga agcauacuua      840

gacaaacagu cagacacgca auacguuugu aaacgcaccu uagucgacag aggcuggggg      900

aacggaugcg ggcuauucgg caagggcucg cuaguaaccu gcgccaaauu cgcaugcagc      960

aagaagauga ccggcaaauc cauacaacca gaaaaccucg aauacagaau aaugcuaucu     1020

guccacggca gccaacacag cggaaugauu gucaacgaca caggccacga aacggacgaa     1080

aaccgagcca aaguagaaau cacacccaac ucaccgcgag ccgaagcaac ccucgggggc     1140

uucggcuccc uaggacucga cugcgaaccc cggaccggcc ucgacuucag cgaccucuac     1200

uaccuaacca ugaacaacaa acauuggcua guccacaaag aaugguucca cgacaucccc     1260

cuuccauggc augcaggagc agacacaggc acaccccacu ggaacaacaa agaagcgcua     1320

guagaguuca aagacgcaca cgcaaaacga caaacagucg ucgugcuggg aucccaagaa     1380

ggcgcugucc auacagcacu cgcaggcgca cucgaagccg agauggaugg cgcaaaagga     1440

cgacuaagcu ccggacaccu caaaugcaga cugaaaaugg acaaacucag acuaaaagga     1500

gucucauacu cccuaugcac cgccgcguuc accuucacca aaauaccagc agaaacacuc     1560

cacggaacgg ucaccgucga aguacaauau gcaggcacag acggaccgug caaagucccg     1620

gcccaaaugg cagucgauau gcaaacacua accccagucg gccgccugau cacugcaaac     1680

cccguaauca cagaaagcac agaaaacucc aaaaugaugc ucgaauugga cccacccuuc     1740

ggggauagcu acaucguaau cggcgucggc gaaaagaaaa uaacacacca cuggcaccgc     1800

agugggagca cuauaggcaa agcauucgaa gccaccguuc gcggagccaa acgcauggcc     1860

guacucggcg acacagccug ggauuucggc ucaguaggcg gcgcccuaaa cucccuaggg     1920

aaaggcaucc aucaaauauu cggagcugcg uucaaaagcc uauucggagg aaugagcugg     1980

uucucccaaa uacucaucgg cacacuacug auguggcucg gccuaaacac caaaaacgga     2040

uccauaagcc uaaugugccu cgcacucggc ggcgugcuca ucuuccucag cacagccgua     2100

agcgcagac                                                             2109


<210>  2805
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2805
aucauaaacg cacgcaagga aaagaaacga agaggcgcug acacaagcgu cgggauagua       60

ggccuccugc uaaccaccgc aauggcagca gaagugacuc gccguggcag cgcauacuac      120

auguaccucg accguaacga cgcaggggaa gcaauaaguu uccccaccac acucggcaug      180

aacaaaugcu acauccaaau cauggaccuc ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcucgacga gggagucgaa ccagacgacg uagacugcug guguaacacg      300

accucaaccu gggugguaua cggaaccugc caccauaaga aaggagaagc ccgccgaucc      360

cgacgcgcag uaacccuccc cucacacucc acccgaaaac uacaaacacg cucacaaaca      420

uggcuggaau cacgcgaaua caccaaacac cucaucagag uagagaauug gaucuuccgc      480

aaccccggcu ucgccuuagc agccgccgcc auagcauggc uacucgggag cucuaccagc      540

caaaagguca uauaccuagu aaugauccug cuaaucgccc cagcauacag cauacgcugc      600

aucggcguau ccaacagaga cuucgucgaa gguauguccg gcggcacaug ggucgacauc      660

guccucgaac acggaggcug cgugaccguc auggcccaag auaaaccaac cgucgacaua      720

gaacucguca ccaccacagu aucaaacaug gccgagguac gcucauacug cuacgaagca      780

uccauauccg acauggcaag cgacucgagg ugcccuacac agggcgaagc cuaccuagac      840

aaacaaucag acacacaaua cgucuguaaa cgcacccuag ucgacagagg auggggaaac      900

gggugcggcc uauucgggaa gggcagccug guaaccugcg caaaauucgc cugcagcaag      960

aaaaugacug gaaaaucgau ccaaccugaa aaccuggaau accgaaucau gcuaagcguc     1020

cacggaagcc agcacucggg caugaucguc aaugacacag gacacgaaac agacgaaaau     1080

cgcgcaaaag uagaaauaac ccccaacucg cccagggccg aagccacgcu cggcggcuuc     1140

ggaucgcuag gccuugacug cgagcccaga accggacuag acuucucaga ucuguacuac     1200

cuaaccauga acaacaaaca cuggcucgug cacaaagaau gguuucacga cauaccccuc     1260

ccauggcacg ccggcgcaga cacaggcaca ccccacugga acaacaaaga agcacucgua     1320

gaguucaaag acgcccacgc aaaacgccaa acagucgucg ugcuaggcuc ccaagaagga     1380

gccguccaca cagcccucgc cggcgcccua gaagccgaaa uggacggagc caaaggacga     1440

cucucaagcg ggcaucucaa augcaggcua aaaauggaua agcucagacu aaaaggaguc     1500

agcuacucac uaugcaccgc cgccuucacc uucaccaaaa uacccgcgga aacacuacac     1560

ggaacaguaa ccgucgaagu ccaauacgcc ggcaccgaug gcccgugcaa aguccccgcu     1620

caaauggccg ucgacaugca aacauuaacg cccguaggcc gccucaucac agcaaaucca     1680

gucauaacgg aaagcacaga aaacagcaaa augaugcucg aacucgaccc gcccuucgga     1740

gacaguuaca ucgucauugg cguaggcgaa aagaaaauaa cacaccacug gcacagaagc     1800

ggaucgacca uaggcaaagc auucgaagca accguccgag gcgccaaaag aauggccgua     1860

cucggagaua cagcauggga cuucggauca gucggcggag cccuaaacuc ccucggcaaa     1920

gguauacacc aaaucuucgg agccgccuuc aaaagccucu ucggaggcau gucgugguuc     1980

agucaaauac ucauaggaac acuacucaug uggcuaggcc uaaacaccaa aaacggcucu     2040

aucagccuca ugugccuagc acuaggaggc guccuaaucu uccucagcac agcgguaucc     2100

gccgac                                                                2106


<210>  2806
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2806
aucaacgccc ggaaagaaaa gaaaaggaga ggcgcagaca caagcgucgg cauagucgga       60

cuccuacuca caaccgccau ggcggccgaa gucacacgga gaggaucggc cuacuacaug      120

uaccuagacc gaaacgacgc cggagaagcc aucagcuucc ccacaacacu aggaaugaac      180

aaaugcuaca uacaaauaau ggaccuuggc cauaugugcg acgccacaau gagcuaugaa      240

ugccccaugc ucgacgaagg agucgagccg gacgacgucg auugcuggug caacaccaca      300

uccacauggg ucgucuacgg cacaugccac cacaagaaag gagaagccag acgcagcaga      360

cgagccguaa cacuacccag ccacucaaca cgcaaacucc aaacccgaag ccaaaccugg      420

cucgagagca gagaauauac caaacaccua aucagggucg agaacuggau auucaggaac      480

ccaggauucg cacuagcugc cgcagcaaua gcauggcuac uaggcagcag caccagccag      540

aaagugaucu accucguaau gauacuucuc auagcacccg ccuacucaau acgaugcaua      600

ggcgucagca accgcgacuu cgucgaaggc augucaggug gaacaugggu agacaucgug      660

cucgaacacg gcggcugcgu caccgucaug gcccaagaca aacccaccgu agacauagag      720

cuagugacca caacaguaag caauauggcc gaagugagaa gcuacuguua cgaagccagc      780

aucagcgaca uggcguccga cucucgcugc ccaacccaag gagaagcgua ccuagacaaa      840

caaagcgaca cacaauacgu augcaaaagg acccucgucg accgaggcug gggcaacgga      900

ugcggacugu ucggcaaagg gagccuagua acaugcgcaa aauucgcgug cuccaagaaa      960

augacaggaa aaagcauaca accagagaac cuagaauacc gcaucaugcu aagcguccac     1020

ggaagccaac acucaggcau gauaguuaac gacacaggcc acgagaccga ugagaaccgc     1080

gccaaagugg agaucacacc aaacucccca agagcagaag cgacccuagg aggauucgga     1140

ucgcucgggc ucgacugcga gccaagaaca ggccuagacu ucuccgaccu cuacuaccua     1200

accaugaaca auaaacauug gcuagugcac aaagaauggu uccacgacau accgcuccca     1260

uggcacgccg gcgcugacac gggaacgccc cacuggaaca auaaagaagc acuggucgag     1320

uucaaggacg cacacgccaa aagacaaacc gucguaguac ucggaagcca agaaggcgcc     1380

guacacaccg cacuggccgg agcccuagaa gcagaaaugg acggcgcgaa aggaagacuc     1440

agcucaggac accugaagug ccggcucaaa auggacaaac uccgacucaa aggaguaagc     1500

uacagccucu gcacagccgc cuucaccuuc accaagaucc cggcagaaac ccuucacggc     1560

accgucacag uagaaguaca auacgcaggu acagacggac ccugcaaggu ccccgcacaa     1620

auggcaguag acaugcaaac ccugacacca guugggagac ugaucaccgc aaacccagug     1680

aucaccgaau caaccgaaaa cagcaaaaug augcuagaac ucgacccacc auucggggac     1740

agcuacauag uaaucggcgu gggagaaaag aaaauaacac accacuggca ccgcucaggc     1800

aguacuauag gaaaagccuu cgaggccaca guccgugggg ccaaaagaau ggcgguacua     1860

ggcgacaccg ccugggacuu cggcagcgua gggggcgcac uaaacucacu cggaaaaggg     1920

auucaccaga uuuucggcgc agccuucaaa agccucuucg gaggaaugag cugguucagc     1980

caaauacuca uaggaacgcu acugaugugg cugggccuca acaccaaaaa cgggucaaua     2040

agccucaugu gccuagcccu aggcggaguc cuaauauucc uauccacagc agucagcgca     2100

gac                                                                   2103


<210>  2807
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2807
aaugcacgaa aagaaaagaa acgacgagga gcagacacuu cagucggcau cgugggccug       60

cuacucacaa cagcgauggc cgccgaaguc acacgccgag gcucugcuua cuacauguac      120

cucgaucgaa acgacgccgg ggaagcaauc ucguucccca ccacgcuggg aaugaacaaa      180

uguuacauac aaaucaugga ccucggccac augugcgacg ccaccauguc cuacgaaugc      240

ccaaugcucg acgaaggagu agagccagac gacgucgacu gcuggugcaa caccaccagc      300

acguggguag ucuacgggac gugccaccau aagaaaggag aagcacgcag aaguagacga      360

gcagucaccc uaccaagcca cucaacccgc aaacuacaaa ccagaucuca aacguggcuc      420

gaaagucgcg aguacacuaa acaccucaua cgcgucgaaa acuggaucuu ccgaaauccc      480

ggauucgcgc uggcagcagc agccauagcc ugguuacugg gcagcuccac cagccaaaaa      540

guaaucuacc uugugaugau ccuacuaauc gcuccagccu acagcauccg augcauaggc      600

gucagcaacc gagacuucgu cgagggcaug uccggcggaa ccuggguaga caucguacua      660

gaacacggcg ggugcgucac agucauggca caagacaaac ccaccgucga cauagagcua      720

guaaccacga caguaagcaa cauggcagaa gucagaagcu acuguuacga agcaucaaua      780

uccgacaugg caagcgacuc ccgaugcccc acccaaggcg aagcauaccu agacaaacaa      840

agcgacaccc aguacgucug caaaaggacc cuagucgacc gaggcugggg aaacgggugc      900

ggacuguucg ggaaaggcag ccucguaacc ugcgccaaau ucgccugcuc aaagaaaaug      960

acgggcaaaa gcauacaacc cgaaaaccuc gaauacagga ucaugcuauc uguccauggc     1020

agccaacacu ccggcaugau agucaaugau acuggacacg aaacagacga aaaccgcgcc     1080

aaaguagaaa ucacacccaa cagcccccgc gcagaagcaa cccucggagg cuucggaucc     1140

cuaggccucg acugcgaacc acgcaccggc cucgacuucu ccgaucucua cuaccucacc     1200

augaacaaua aacacuggcu gguccacaaa gaaugguucc acgacauacc ccugccgugg     1260

cacgccggcg ccgacacagg aaccccacac uggaacaaca aagaggcgcu agucgaguuc     1320

aaagacgccc acgcuaaacg acaaacgguc gucguccuag gcucccaaga aggggcgguc     1380

cacacagccc ucgcaggagc ccuggaagcc gaaauggacg gcgccaaagg cagacucagc     1440

uccggccacc uaaagugccg ccucaaaaug gacaaacuaa gacugaaagg cgucagcuac     1500

agccuaugca cagcagcauu cacauucacc aaaauacccg ccgaaacccu gcacggaaca     1560

gugacaguag aaguccaaua cgccggcacc gacgggcccu gcaaaguacc agcccaaaug     1620

gccgucgaca ugcaaacgcu caccccaguc ggccgacuga ucaccgccaa cccagucauc     1680

accgaaucca cagagaacuc aaaaaugaug cucgaacuag aucccccauu cggcgacucc     1740

uacauaguua ucggcguugg agaaaagaaa aucacgcacc acuggcacag gagcgguuca     1800

acaauaggaa aagccuucga agcgacaguc cgcggagcca aacgaauggc gguacugggc     1860

gacacugcau gggacuucgg cagcguaggc ggcgcucuca acucccuggg aaaaggaaua     1920

caucaaauau ucggagccgc auuuaaauca cuguucggag gcaugucaug guucagccag     1980

auccuaauag gcacccuccu cauguggcuc ggacuaaaca cgaaaaaugg aagcauaagc     2040

cuuaugugcc uagcacucgg cggaguccua auauuccuca gcacggccgu gagcgccgac     2100


<210>  2808
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2808
gcccgaaaag aaaaaaaacg acgaggcgcc gacacauccg uaggaauagu cggccuccua       60

cuaaccacgg cuauggcagc cgaaguaacg cgccgaggau cagccuacua cauguacuua      120

gacagaaacg acgcaggaga agcaaucagc uucccaacca cgcucggaau gaauaaaugc      180

uacauccaaa uaauggaccu aggccacaug ugugacgcca cuauguccua cgaaugcccc      240

augcuagacg agggcgucga gccagacgac gucgauugcu ggugcaacac caccagcacc      300

ugggucgugu acgggacaug ccaccacaaa aaaggcgaag cacgacgauc ccgccgagca      360

gucacccugc caucccauag cacaagaaaa cuccaaaccc gcagccagac auggcuagaa      420

agccgagaau acacaaaaca ccucauccga guggaaaacu ggaucuuucg caacccaggc      480

uucgcacucg cagcagccgc caucgccugg cuccuaggcu cgagcaccag ccaaaaaguc      540

auauaccucg ucaugauacu acucauagcc ccggcauacu ccauccgaug cauuggaguc      600

agcaaccggg acuucgucga aggaaugagc ggcggcaccu ggguagacau cgugcucgaa      660

cacggaggcu gcguaaccgu cauggcgcag gacaaaccca caguagauau cgaacuaguc      720

accacuacag uaagcaacau ggcggaagua cgaagcuacu guuacgaggc cagcauaucc      780

gacauggcau cggacagcag augccccacc caaggggagg cauaccucga caaacaauca      840

gacacgcaau acguaugcaa acggacccug gucgaucgcg gauggggcaa cgggugcggc      900

cucuucggca aaggcucacu cgucaccugc gccaaauucg caugcuccaa gaaaaugacc      960

ggcaaaagca uacaaccaga gaaccuagaa uaccgaauca ugcugucagu ccacgguagc     1020

caacacagcg gcaugaucgu caacgacacc ggacacgaaa ccgacgaaaa uagagccaaa     1080

guagaaauca cgcccaacag cccaagagca gaagccaccc ucggcggguu cgguagccua     1140

ggccuugacu gcgagccgcg cacaggacug gacuucagcg accucuacua ccugaccaug     1200

aacaauaaac acuggcucgu acacaaagag ugguuccacg acaucccccu acccuggcac     1260

gcaggagccg acaccggcac gccacauugg aacaacaaag aagcccucgu cgaauucaaa     1320

gacgcgcaug cgaaaagaca gacagucgua guccuaggau cacaagaagg agcgguacau     1380

acagcacuag caggagcgcu cgaagcagaa auggacggcg caaaaggacg acuaucguca     1440

ggccaccuca aaugccgacu caaaauggac aaacuaagac ucaaaggggu cagcuacucc     1500

uuaugcaccg ccgcauucac auucaccaaa auccccgcag aaaccuuaca cggaaccgug     1560

accgucgaag uacaauacgc aggaaccgau ggacccugca aagucccagc acagauggcc     1620

guagauaugc aaacccugac accggucggc cgacuaauaa cagcaaaccc cguaaucacu     1680

gaaucaaccg aaaacucaaa aaugaugcua gaacuugacc cacccuucgg cgacagcuac     1740

aucguaaucg gcguaggcga gaaaaaaauu acacaccacu ggcaccgauc cggaagcacg     1800

aucggcaagg cauucgaagc gacgguccga ggagccaaac gaauggcagu ccucggcgac     1860

acagcguggg acuucggcag cgucggcggc gcucucaaca gccuaggcaa aggcauccac     1920

cagaucuucg gcgccgccuu caaaucccua uucggcggca ugucaugguu cagccaaauc     1980

cuaauaggca cacuccucau guggcucggc cucaacacaa aaaacggcuc aauaagccua     2040

augugccucg cccucggcgg cguucucauu uuccucagca ccgcagucag cgcggac        2097


<210>  2809
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2809
cggaaagaaa agaaacgccg aggcgcagac acgagcguag gaauagucgg ccuacuccug       60

acgacagcaa uggcagcaga aguaacacgc cguggaagcg cguacuacau guaccucgac      120

cggaacgacg caggagaagc caucagcuuc ccaaccaccc ucggaaugaa caaaugcuac      180

auacaaauaa uggaccuagg acacaugugc gacgccacca uguccuacga gugccccaug      240

cuagacgaag gaguagaacc agacgacgua gacugcuggu gcaacacaac auccacgugg      300

guaguauacg gaacaugcca ccacaagaaa ggcgaagcac gccggagccg acgagccguc      360

acgcugccau cccacagcac acgaaaacua caaacccggu cccagaccug gcuagaauca      420

agagaauaca caaaacaccu cauacgggua gagaacugga uauucagaaa ccccggguuc      480

gcacucgcag ccgccgcaau agccuggcuc cugggcucaa gcaccuccca aaaagugaua      540

uaccuaguca ugauacugcu gaucgccccg gccuacucca ucagaugcau cggggucagc      600

aacagagacu ucgucgaagg uaugucugga ggaaccuggg uagacauagu acuagaacac      660

ggaggaugcg ucacggucau ggcucaggac aaacccaccg uagauauaga acuggucacc      720

acaacgguaa gcaauauggc cgaaguaaga uccuacugcu acgaggccag cauaucagac      780

auggcauccg acucacgcug cccaacccaa ggagaagcau accucgacaa acaaagcgau      840

acgcaauacg ugugcaaaag aacccuagua gaccgaggau ggggaaacgg cugcggccug      900

uucggcaaag ggucacuggu aaccugugcg aaauucgcgu gcucaaagaa aaugacaggg      960

aaauccaucc aacccgaaaa ccucgaguac cgaauaaugc uaagcgucca cgggucacaa     1020

cacuccggaa ugaucgucaa cgacacagga cacgaaacag acgaaaacag agcaaaagua     1080

gaaaucacac cgaacagccc ccgugcugag gcaacacucg ggggauucgg gucccuagga     1140

cucgacugcg aaccacgaac gggacuagac uucagcgacc uauacuaucu cacgaugaac     1200

aacaaacacu ggcuagugca caaagaaugg uuccacgaca ucccccuacc cuggcacgca     1260

ggagccgaca cuggcacacc ccacuggaac aacaaagaag cacugguaga guucaaagac     1320

gcacacgcca agagacagac cguagucgua cucggaagcc aagagggggc ggugcacaca     1380

gcacucgcag gagcgcucga agcagagaug gacggggcca aaggccgacu auccagcgga     1440

caccucaaau gccgacuaaa aauggacaaa cucagacuaa aaggagucuc cuacagccua     1500

ugcacagcag ccuucaccuu caccaagaua cccgcagaaa cacuccacgg caccgucacc     1560

gucgaaguac aauacgccgg gaccgaugga ccaugcaaag ucccagcgca aauggcagug     1620

gacaugcaaa cccuaacccc agucggacgu cugaucaccg caaaucccgu caucacagaa     1680

uccaccgaaa auagcaaaau gaugcuggaa cuggacccac ccuucggcga caguuacaua     1740

gucaucggag ucggagaaaa gaaaauaaca caccauuggc acagguccgg gagcaccaua     1800

ggcaaagcau ucgaagcaac aguacgagga gccaaaagaa uggccgucuu gggagacacu     1860

gcaugggacu ucggcuccgu aggaggagca cucaacagcc ucggaaaagg aauccaccag     1920

auauucggcg cagcauucaa aucccucuuc ggaggcauga gcugguucuc ccaaauacuu     1980

auaggcacgc uccuaaugug gcuaggccuc aauaccaaaa acggaagcau cucucuaaug     2040

ugccucgcgc uaggaggcgu ccuaauauuc cuaucaacag caguuucagc agac           2094


<210>  2810
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2810
aaagaaaaga aacgccgagg ggcggacaca agcgugggua ucgucggccu acucuuaacc       60

accgcgaugg ccgcggaggu cacccgacga ggcuccgcgu acuacaugua cuuagaccga      120

aaugacgccg gcgaagccau aucauucccc accacgcucg gcaugaacaa augcuacauc      180

caaauaaugg accucggcca caugugcgac gccaccauga guuaugaaug ccccaugcuc      240

gacgaagggg uagagccaga cgacguagac uguuggugca acacaaccag cacauggguc      300

guguacggaa ccugccacca caagaaaggc gaagcccggc gcagccgccg ggcagucaca      360

cuccccagcc auagcacccg caaacuacag acccgcucgc aaaccuggcu cgaaucaaga      420

gaauacacca aacaccucau ccgcgucgaa aacuggauau uucgaaaccc cggauucgcc      480

cuggcagcag cagcaauagc cuggcuucuc ggcuccagca caagccagaa agucaucuac      540

cucguaauga uccuauuaau agccccagcu uacuccaucc gguguauagg ggucuccaac      600

agagacuucg ucgaaggcau guccggugga accuggguag acaucguacu cgaacacgga      660

ggaugcguca ccgucauggc ccaagacaaa cccaccgugg acaucgaacu cgucacaacc      720

acagucucga acauggccga agucaggucc uacugcuacg aagcgucaau aucagacaug      780

gcauccgaca gccgaugccc aacccaggga gaagcauacc uugacaaaca aucggacacc      840

caauacgucu gcaagcgcac acucguggac agaggauggg gcaacggaug cggacuauuu      900

ggcaaaggga gccuaguaac cugcgccaaa uucgcgugca gcaagaaaau gacuggcaaa      960

aguauccaac cagagaacuu agaauaucga aucaugcuca gcguccacgg aagccaacac     1020

uccggcauga ucgucaacga cacaggccac gagacagacg aaaaccgugc caaaguagaa     1080

auaacaccaa auagcccccg agcagaggca acccucggag gcuucgggag ccuaggacuc     1140

gacugcgagc cgaggacggg acuagauuuc agcgaccuau acuaccuaac aaugaacaac     1200

aaacauuggc uuguccacaa agaaugguuc cacgacaucc cacuccccug gcacgccgga     1260

gccgacacug gcacaccuca cuggaacaac aaagaagcac ucguagaguu caaggacgcc     1320

cacgcuaaac gccaaacagu uguaguacuc ggcagccaag aaggagccgu ccacacagca     1380

cucgcaggcg cccucgaagc cgaaauggau ggagccaagg gccgacuauc gucaggacac     1440

cucaagugcc ggcucaagau ggacaaacuc agacugaaag gaguaagcua cagccuaugc     1500

accgccgccu ucaccuucac caagaucccc gcagaaacac uccacggaac agucaccguc     1560

gaaguacagu acgccggaac cgacggcccc ugcaaagucc ccgcacaaau ggccguggac     1620

augcagaccc uaacaccagu aggacgccuc auaacagcaa auccaguaau aaccgaguca     1680

accgaaaaca gcaaaaugau gcuagaacua gauccacccu ucggcgacuc cuacauagua     1740

aucggcguag gcgagaagaa aauaacacau cacuggcaca gaucagguuc cacaaucggg     1800

aaagcauucg aagccacagu ccgcggagcc aaaagaaugg cgguacuagg cgacacagcc     1860

ugggacuucg gaaguguagg uggcgcgcuc aacucccuag ggaagggaau acaccaaauc     1920

uucggcgccg cauucaaaag ccuauucggc ggaaugagcu gguucagcca aauucucaua     1980

ggcacccucc uaauguggcu uggacucaau acaaagaacg ggagcauauc ccuaaugugc     2040

cucgcccuag gcggcguccu aauauuucug agcacagcag ucagcgcaga c              2091


<210>  2811
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2811
gaaaagaaga ggcgaggagc cgacaccagc guagggauag uaggccuacu ccucacaaca       60

gcuauggcag cagaagucac acgacgcggc agugcauacu acauguaccu cgaccgaaac      120

gaugccggcg aggccaucag cuuccccacc acacuaggca ugaacaaaug cuacauacaa      180

aucauggacc ucggacacau gugcgacgca acaauguccu acgaaugccc aaugcucgac      240

gaggggguag aacccgacga cguagacugc uggugcaaca cgaccuccac augggugguu      300

uacggcacau gccaccacaa gaaaggcgaa gcccgacgaa gccgacgugc agugacacuc      360

ccaucgcacu ccacacgaaa acuacaaaca cgaucucaaa cauggcuaga aucccgcgaa      420

uacacaaaac accuaauacg gguggaaaac uggauauuca gaaaccccgg cuucgcccua      480

gcagcagccg caauagcaug gcucuuaggg agcuccaccu cacaaaaagu caucuaccuc      540

guaaugaucu uacuaauagc gccggccuac agcauacgcu gcauaggggu aagcaaccga      600

gacuucgucg aaggaaugag cggaggcacc uggguagaua ucguacuaga gcacggcggc      660

ugcgucacug uaauggcaca agacaaaccu acaguagaca ucgaauuggu aacaacaaca      720

gucagcaaca uggccgaagu cagaucauac ugcuacgaag cgagcauaag cgacauggca      780

ucugacagcc gcugccccac ccagggcgaa gccuaccucg acaaacaguc agacacccaa      840

uacguaugca aaagaacacu cguagaccga ggauggggca auggaugcgg acucuucggg      900

aaaggaagcc uagucacaug cgccaaauuu gcaugcucaa agaaaaugac aggaaaaucg      960

auccaaccag aaaacuuaga auaucgaauc augcugagcg uacacggcag ccaacacagc     1020

ggcaugauag ucaacgacac uggacacgaa accgacgaaa accgggcaaa agucgaaauc     1080

accccaaacu caccaagggc cgaagcaaca cucggcggcu uuggaagccu aggccucgac     1140

ugcgaacccc gcaccggacu agacuucuca gaccuauacu accuaaccau gaacaacaaa     1200

cacuggcuag uucacaagga augguuccac gacauacccc ucccauggca cgccggcgca     1260

gacacaggaa cgccccacug gaacaacaaa gaagcacucg uagaguucaa ggacgcccac     1320

gccaagcggc agacaguagu cgugcucgga ucacaagaag gagccgucca cacagcccuc     1380

gccggagccc uugaagcgga aauggacggc gcaaaaggac ggcugucuag uggacaccua     1440

aaaugucggc ugaaaaugga caaacugcgu cuaaaaggug uaagcuacag ccuaugcaca     1500

gcagcguuca ccuucaccaa aaucccggca gaaacgcucc acggaaccgu cacaguagaa     1560

guacaauacg cagggacaga cggcccaugc aaaguacccg cccaaauggc cguagacaug     1620

caaacacuaa caccagucgg aagacucaua acagccaacc ccguaaucac agaaagcacc     1680

gagaacucca aaaugaugcu ggaacucgau ccucccuucg gcgacagcua uauagucaua     1740

ggugucggug agaagaaaau aacacaccac uggcaccgca gcggcagcac aaucggaaaa     1800

gccuucgaag caaccguucg gggagcgaaa cgaauggccg ucuuaggcga cacagcaugg     1860

gacuucggcu cuguaggcgg agcacugaac agccuaggca aaggaaucca ccagauauuc     1920

ggcgcagccu ucaaaucacu cuucggcggg augucuuggu ucucacaaau acucauagga     1980

acacuccuca uguggcuagg cuuaaacacc aaaaacggau cgaucucgcu gaugugccua     2040

gcacucggcg gagugcugau auuccuaucg accgcaguau cggccgac                  2088


<210>  2812
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2812
aagaaacgcc gcggcgccga caccagcgua ggaaucgucg gacuacuguu gacaaccgca       60

auggccgcgg aagugacacg aaggggaagc gccuacuaca uguaccucga cagaaacgac      120

gccggagaag ccauauccuu cccgaccaca cuaggcauga acaaaugcua cauacaaaua      180

auggacuuag gccauaugug cgacgcaacg augucauacg aaugcccuau gcuagacgaa      240

ggaguggaac ccgacgacgu cgacugcugg ugcaacacga caucgaccug gguagucuac      300

ggcacuugcc accacaagaa aggagaagca cgacgaagcc gacgggcagu cacgcuaccc      360

agccacucca cccgaaaacu ccaaacucgc agccaaaccu ggcucgaaag ccgcgaauac      420

accaagcacc uaauacgagu cgaaaacugg auauuccgga acccaggcuu cgcccucgca      480

gcagcagcaa uagccuggcu ccuuggaagc uccacauccc aaaaaguaau auaccuaguc      540

augauacucc uaaucgcccc cgcauacagc auccgaugua ucggaguauc caaccgcgac      600

uucgucgagg gcaugagcgg cggcaccugg guagacauag uccucgaaca cggaggcugc      660

gucacaguaa uggcccaaga caaacccaca guagacaucg agcuggucac cacaacggua      720

agcaacaugg ccgaaguccg auccuacugc uacgaagcaa gcauaucaga cauggcauca      780

gacagcagau gccccacaca aggcgaagcc uaccuagaca aacaaagcga cacacaauac      840

guaugcaaac ggacguuggu cgaccggggc uggggcaacg gaugcggucu cuucggaaaa      900

ggcagccucg uaaccugcgc caaauucgcg ugcucaaaga aaaugacggg caaaucaauc      960

caaccggaaa aucuagagua ccggauaaug cuaagcgucc acggaucaca gcacuccggc     1020

augaucguca acgacaccgg acacgaaaca gacgaaaacc gugcaaaagu ggagaucaca     1080

ccaaacagcc cccgcgccga agccacacua ggcggauucg gaucccuggg acuagacugc     1140

gaaccaagga caggacuaga cuucuccgac cucuacuacc ucacaaugaa uaacaaacac     1200

uggcuaguac acaaagaaug guuccacgac aucccacucc ccuggcacgc cggagcagac     1260

acaggcacac cacacuggaa caacaaagaa gcacucgucg aguucaaaga cgcacaugcc     1320

aaacgccaaa ccgucguagu gcuaggaucc caagaaggag caguccacac cgcgcucgcc     1380

ggagcccugg aagccgaaau ggacggagca aaagggcgcc ugaguuccgg ccaccuaaag     1440

ugccgccuaa agauggacaa acuaagacuc aaaggcgucu ccuacagccu cugcaccgca     1500

gcguucaccu ucacuaaaau cccggccgaa acacugcacg gaacaguaac cguggaggug     1560

caguacgcag gcacagacgg acccugcaaa gugccggccc aaauggcagu cgauaugcaa     1620

acacuaacgc ccguaggacg acucaucaca gccaaccccg uaaucacaga guccacagaa     1680

aacucaaaaa ugaugcuaga acuagauccc ccauucggag acagcuacau agucaucggc     1740

gucggugaaa agaaaauaac acaccauugg cacagaagcg gcucaaccau cggaaaagcc     1800

uucgaagcca cgguccgagg ggcaaaacgc auggccgucc uaggggacac ggcaugggac     1860

uucgggagcg uagggggagc ccucaacucc cugggcaagg gaauacacca aauauucggc     1920

gccgcuuuca aaagccucuu cggcgggaug agcugguuca gccaaauccu caucggaaca     1980

cuucucaugu ggcucggucu aaacaccaaa aacggaagca uaucgcugau gugccucgcg     2040

cuuggcggcg uccuuauauu ccuaucaacc gccguuuccg ccgac                     2085


<210>  2813
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2813
aagcgccgcg gagccgacac aagcguagga auugucggcc uccuucuuac aacagcgaug       60

gcggccgaag uaacccgaag aggcuccgcc uacuauaugu accucgacag gaacgacgcc      120

ggcgaagcaa ucucauuccc cacaacccug ggaaugaaca aaugcuacau ccagauuaug      180

gaccuaggcc acaugugcga ugccaccaug ucguacgaau gccccaugcu agacgaagga      240

guagaaccag acgacguaga cugcuggugc aacacaacca gcacaugggu cguauacgga      300

acaugccacc acaaaaaggg cgaagcgcga agaagccgcc gcgcagugac ccucccaagc      360

cacagcacac gaaaacucca aacacgcagc caaacauggc ucgaaagccg ggaauacacc      420

aaacaccuca ucagggucga aaacuggauc uuccgaaacc ccggcuucgc acucgcugca      480

gcagcaauag ccuggcuucu cggauccagc accucccaaa aagucauaua ccuagucaug      540

auacuccuaa uagcccccgc auacucuaua cgcugcauag gagugaguaa ccgagacuuc      600

guggaaggga uguccggcgg aacauggguc gacaucgucc uagaacacgg uggcugcgua      660

acaguaaugg cacaagacaa acccacagug gacaucgagc uuguaacaac cacaguaucc      720

aacauggcag aagucagauc cuacugcuac gaagccucca uaucugacau ggccucagac      780

ucacgaugcc caacccaagg agaagcauac cuagacaaac aaagcgacac acaauaugua      840

ugcaaaagaa cauuagucga ccgcggaugg ggaaacggau gcggacuuuu cggaaaaggu      900

ucccuaguga cuugcgccaa auucgccugu ucgaagaaaa ugaccggcaa guccauccaa      960

ccggaaaacc uggaauaccg aaucaugcua ucuguccacg gcucgcaaca cucaggcaug     1020

aucguaaacg acacgggcca cgaaacagac gaaaacagag ccaaagucga gauaaccccc     1080

aacuccccuc gcgccgaagc cacgcucggc ggauucggaa gccugggccu cgacugugaa     1140

ccgcgaacag gacuagacuu cagcgaccuc uacuaccuaa ccaugaacaa caagcacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccacucccau ggcacgcagg agccgacacc     1260

ggaacaccac acuggaacaa caaggaagcc cucgucgagu ucaaagacgc gcacgccaaa     1320

cgccaaacag uggucguccu aggaucccaa gaaggcgccg uccacaccgc acuagcggga     1380

gcccucgaag ccgaaaugga cggcgcaaaa ggaagacuca gcucgggcca ccuaaaaugc     1440

agacuaaaaa uggauaagcu ccgacugaaa ggaguaucuu acucccuaug cacagcagca     1500

uucaccuuua cuaaaauccc cgcagaaaca cugcacggaa caguaaccgu cgagguacaa     1560

uacgcaggca ccgacggacc cugcaaagua ccggcccaaa uggccgucga caugcaaacc     1620

cucacaccag ucggaagacu caucacggca aaccccguaa ucaccgaauc caccgaaaac     1680

ucuaaaauga ugcuggagcu agacccaccc uucggcgaca gcuacaucgu caucggagug     1740

ggagagaaga agaucaccca ccacuggcac cgaagcggca gcaccaucgg caaagcauuc     1800

gaggcgacug uccgcggagc gaaaagaaug gccguucucg gggacaccgc augggacuuc     1860

ggguccgucg gcggcgccuu aaacagucuc ggaaaaggca uacaccaaau auucggcgcc     1920

gcguucaaaa gccuguucgg aggcaugucc ugguucucgc aaauacuaau cggcacccuc     1980

cuuauguggc ucgggcuaaa caccaaaaac ggaagcauaa gccuaaugug ccuagcccua     2040

ggcggcgugc ucaucuuccu uagcacagcc guaagcgcgg ac                        2082


<210>  2814
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2814
agacggggcg cagacacaag cgucggaauc gucggacucc uacuuacgac cgcaauggcg       60

gcugaaguca cacgccgagg auccgcauac uacauguacc ucgaccguaa cgacgcaggc      120

gaagccauau cauucccuac aacgcuggga augaacaaau gcuacaucca gauaauggac      180

cuaggccaca ugugcgacgc aaccaugucc uacgaaugcc caaugcucga cgaagggguc      240

gaacccgacg acguagacug cuggugcaac acaacgagca cauggguagu guacggaaca      300

ugccaccaca aaaaggggga agcccgacga uccaggagag cagugacacu cccaagccac      360

agcacaagaa aacuacagac aagaucacaa accuggcuug aaagcagaga auacacaaag      420

caccuaauac gaguugaaaa cuggauauuc agaaaccccg gcuucgcacu cgcagcagcc      480

gccauagccu ggcuccuggg caguucaacg agccaaaaag ucauauaccu aguaaugaua      540

cugcuaaucg ccccagcaua cucaauacgc ugcaucggcg uauccaaccg agacuucgua      600

gaaggaaugu caggaggaac cuggguagac auuguacucg aacacggagg augcguaaca      660

gucauggccc aggacaaacc cacggucgac aucgaacucg uaacaacaac cguaucaaac      720

auggcagaag uacgauccua cugcuacgaa gcaagcaucu cugacauggc cucagacuca      780

cgaugcccca cccagggcga agccuaccua gacaaacagu cggacacaca auacgucugc      840

aaacgaacac uggucgaccg cgguuggggc aacggcugcg gccuguucgg aaaagggagc      900

cuagugaccu gcgccaaauu cgccugcagc aagaaaauga ccggcaaauc gauacaaccc      960

gaaaaccuug aguacagaau caugcuaucc guccacggca gccaacauuc cggcaugaua     1020

gucaacgaca ccggccacga aacagacgaa aacagagcaa aagucgaaau cacgcccaac     1080

uccccacggg cggaagccac acugggagga uucggcagcc ucggccuaga uugcgaacca     1140

cgcacagggu uagacuucuc cgaccucuac uaccuuacaa ugaacaacaa acauuggcuc     1200

gugcacaaag aaugguucca ugacauccca cuccccuggc augcaggcgc cgacacgggg     1260

acaccacacu ggaauaacaa ggaggcgcuu guggaguuca aggaugcgca cgccaaacga     1320

caaacaguag uuguccuagg cucacaagaa ggcgcggucc acacagcccu cgccggggca     1380

cuagaagccg aaauggacgg cgccaaaggg cgacucagca gcggccaccu caaaugccga     1440

cuaaaaaugg acaaacugcg ccuaaaaggg gucagcuaca gccucugcac cgccgcauuc     1500

accuucacaa aaauaccagc cgaaacccua cacggcacag ucacaguaga aguacaauac     1560

gccggcacag acggacccug caaaguccca gcccaaaugg cggucgacau gcaaacccuc     1620

acgccaguag gccgacuaau cacagcaaac ccagucauca ccgagagcac agaaaacagc     1680

aaaaugaugc uagaacugga cccgccauuu ggagauaguu acaucguaau uggcgugggg     1740

gaaaagaaaa uaacacacca cuggcacaga agcggcucca caaucggaaa agcauucgaa     1800

gcuaccgucc gaggagcgaa acgaauggcc guacucggcg acacagccug ggacuucggc     1860

ucaguaggcg gagcccugaa cucccucggc aaaggaauac accaaaucuu cggggccgcg     1920

uucaaauccc uguucggagg aaugagcugg uucucacaga uacucauagg cacacuacua     1980

auguggcuag gccuaaacac aaagaacggc agcauuagcu ugaugugccu agccuuggga     2040

ggcguacuaa uauuccuaag cacagccgua ucagccgac                            2079


<210>  2815
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2815
cgaggagccg auacuucggu cggcauaguc ggccuacucc uaaccacggc cauggcugca       60

gaagucacaa gacggggcuc agccuacuac auguaccucg acagaaacga ugcaggagaa      120

gcaaucucgu uccccacaac acugggcaug aacaaaugcu auauacaaau cauggaucuu      180

ggccacaugu gcgacgcaac caugagcuac gagugcccaa ugcucgacga aggcgucgaa      240

cccgaugacg uggacugcug gugcaacaca accagcacau gggucguaua cggaaccugc      300

caccacaaga aaggcgaagc ccgccgauca cggcgcgcag ugacccuccc cucacacucc      360

acccgaaaac uacaaacaag aagccaaacc uggcucgaau cacgcgagua caccaaacac      420

cuaaucagag uagaaaacug gauauuccgc aacccaggcu ucgcacuggc cgccgcggca      480

auagcauggc uccuuggcuc aagcacauca caaaaaguaa uuuaccucgu aaugauacua      540

cucaucgccc ccgccuauag cauccgcugc aucggcgucu caaaucgcga cuucguagaa      600

ggcaugagcg gcggaacaug gguagacauc guccucgaac acggagguug cgucacgguc      660

auggcccaag acaaacccac aguagacauc gagcuaguca ccacgacagu cucuaacaug      720

gcugaaguac gaagcuauug cuacgaagcg aguaucuccg acauggcauc agacucacgu      780

ugccccaccc aaggagaggc cuaccucgac aaacaauccg acacacaaua cgucugcaaa      840

cgcacccucg uagacagagg augggggaac ggcugcggac uguucggcaa aggaagccuc      900

guaacaugcg caaaauucgc augcucaaag aaaaugaccg gaaaaucaau acaaccagaa      960

aaccucgaau accgcauaau gcuauccgua cacggcuccc aacacuccgg aaugauagua     1020

aacgacaccg gccacgaaac cgacgagaac cgagcaaaag ucgagaucac cccuaacucc     1080

ccacgcgcag aagcaacccu aggcggcuuc ggcucccucg gacuggacug cgaacccaga     1140

accggacugg acuucucaga ccucuacuac cucacaauga acaacaaaca cuggcucguu     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg caggcgcgga cacagguacg     1260

ccacacugga acaauaagga ggcacucguc gaguucaaag acgcacacgc aaaacgccaa     1320

acugucgucg uacugggcuc ccaagaagga gccguacaca cagcccucgc cggggcacuc     1380

gaagccgaaa uggacggcgc caagggacga cuaagcuccg gccaccucaa augccgacua     1440

aaaauggaca agcuacggcu caaaggcguc ucguacagcc ucugcacagc ggccuucacc     1500

uucaccaaaa uccccgccga aacucuacac ggcacaguca ccguagaagu ccaguacgca     1560

ggaaccgacg gccccugcaa ggugccagca caaauggccg uagauaugca aacccuuacc     1620

cccguaggcc gccucauuac cgccaacccc gugauaaccg agagcaccga aaacagcaaa     1680

augaugcuag aacucgaucc cccauucggc gacaguuaca ucgucaucgg cgucggagag     1740

aagaaaauca cacaccacug gcacagaucc gguucaacaa uaggaaaagc cuucgaagca     1800

accguccgcg gagccaaacg cauggcagua cucggcgaca ccgcauggga cuucggaucc     1860

gucggaggag cccuaaacuc ccucggcaag ggaauacacc aaaucuucgg cgccgccuuc     1920

aaaagccucu ucgguggaau gucaugguuc agccaaaucc ugaucgggac acuucuaaug     1980

uggcuuggcc uaaacaccaa aaacggcagc aucagucuca ugugucuagc ccucggcgga     2040

guacucauau uccuaucaac agcaguaucc gccgac                               2076


<210>  2816
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2816
ggggcggaua ccuccguagg aauagucgga cuacuacuca cuaccgcaau ggccgcagag       60

guaacacgac gcggcagcgc cuacuacaug uaccuagacc gaaacgacgc aggagaagca      120

aucagcuucc ccacgacacu gggaaugaac aagugcuaca uacagaucau ggaccucggc      180

cacaugugcg acgcaaccau gagcuacgaa ugcccaaugc uagacgaagg aguagaaccc      240

gacgacguag acuguuggug uaacaccaca agcaccuggg ucgucuacgg aacaugccac      300

cacaagaagg gagaggcccg ccgcagcaga cgagccguaa cacuacccuc gcacagcacc      360

agaaaacuac agaccagaag ccaaacaugg cuagaaagca gagaauacac aaaacaccua      420

auccggguag aaaacuggau auuuaggaac ccgggauucg cacuagcagc cgcagcaaua      480

gcauggcuac uagggagcuc aaccucccaa aaaguaauau accuagucau gauccuacuc      540

aucgcgccag cauacuccau ccggugcaua ggaguaucaa acagagauuu cguagaaggc      600

augucagggg gcaccugggu agacauaguc cucgaacacg gagggugcgu aacggugaug      660

gcacaagaua aaccaaccgu agacaucgag cuaguaacaa caacagucag caacauggcc      720

gaaguaaggu cguacugcua cgaagccagu aucucagaca uggccagcga cucacgaugc      780

ccaacccaag gagaagccua ccuagacaaa caguccgaca cacaguacgu augcaaacgc      840

acgcucgucg accgaggcug gggaaacggc ugcggccugu ucggaaaagg gagccugguc      900

acaugcgcaa aauucgccug cuccaagaaa augaccggaa aauccauaca acccgaaaac      960

cuagaauacc ggaucaugcu caguguccac ggcagucagc auagcggcau gaucguaaac     1020

gacaccggac acgaaacaga ugaaaaccga gcaaaagucg aaauaacccc aaacucgccc     1080

cgagcagaag ccacauuagg cggcuucgga ucccuaggac uagacugcga accccgaacc     1140

ggacucgacu ucucagaccu guauuaucuc acgaugaaca acaaacacug gcugguacac     1200

aaggaauggu uccacgacau accacuacca uggcacgcug gagcugacac cggaacacca     1260

cacuggaaca acaaagaggc ccuaguagag uucaaagaug cacaugccaa acgccaaacc     1320

gugguagucc uaggguccca agaaggagcc gugcacaccg cacucgccgg cgcacuggaa     1380

gcagaaaugg acggagccaa agggcgacua agcuccggcc accuaaaaug cagacucaaa     1440

auggacaaau uacgacuuaa aggcguaagc uauucacugu gcacggcggc auucacauuc     1500

accaaaauac ccgccgaaac ccuacacgga acagucaccg uagaagucca auacgccggg     1560

acagacggac cgugcaaagu cccagcccaa auggccguag acaugcaaac acucaccccc     1620

guaggaagac ucauaaccgc aaacccgguc aucaccgaau caaccgaaaa cagcaaaaug     1680

augcucgaac uagacccgcc cuucggggac agcuauauag ugauaggcgu aggagaaaag     1740

aaaauaacgc accacuggca ccgaagcggc agcacaauag ggaaagccuu cgaagccaca     1800

guacgaggag cgaaaagaau ggcaguccug ggagacaccg cgugggacuu cggaucggua     1860

ggaggcgcac uaaacagccu gggaaaagga auacaccaaa ucuucggcgc cgccuucaaa     1920

ucacucuucg gcggaauguc augguucucc cagauacuaa ucgggacccu ccugaugugg     1980

cuaggucuaa acaccaaaaa uggcucaauc ucacuaaugu gccuagcgcu aggaggcguc     2040

cucaucuucc uguccacagc cguaucggcc gac                                  2073


<210>  2817
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2817
gcagacacca guguaggcau cguaggacuc cuacuaacca cagcaauggc cgcugaaguc       60

acacgacgcg gaagcgcaua cuacauguac cuagaccgua acgacgccgg cgaagcaauc      120

ucguucccca cgacccucgg aaugaacaaa ugcuacauuc aaauuaugga ccuagggcac      180

augugcgacg cgacaaugag cuacgaaugc cccaugcucg acgaaggcgu ggaaccagac      240

gacgucgacu gcuggugcaa cacaacaagc acaugggugg ucuauggaac cugccaucac      300

aagaaaggcg aagcacgaag aagccgccgg gcaguaacac uuccgaguca cagcacccga      360

aaacuccaaa cacgcagcca aacauggcua gaaagccgcg aauauacgaa acaccuaauc      420

agaguagaaa acuggauauu ccgaaacccu ggcuuugcac ucgccgcagc agccauagca      480

uggcuccugg gcagcagcac aagccaaaaa gucaucuacc uagucaugau acuccuuauc      540

gcccccgcau acucaauccg cugcaucggc gucucgaaca gggacuucgu ggagggcaug      600

agugggggaa ccuggguaga caucguccua gaacacggcg gcugcgucac gguaauggca      660

caggacaaac caacaguaga uauagagcua guaaccacua caguauccaa cauggcagaa      720

guccgcagcu acugcuacga agcaucgaua ucggacaugg ccagcgauuc cagaugccca      780

acccaagggg aggccuaccu cgacaaacaa agcgacaccc aguacguaug caaaagaaca      840

cucguggaca gagggugggg caacggcugc ggacuauucg gcaagggauc acugguaacc      900

ugcgccaaau ucgccugcuc caagaaaaug accggcaaaa gcauacagcc agaaaaccua      960

gaauacagaa ucaugcucuc cguacacggc ucgcaacaca gcggaaugau cguaaacgac     1020

acaggacacg aaacagacga aaaccgcgca aaagucgaaa ucaccccgaa cuccccgcgc     1080

gcagaagcaa cacuaggcgg cuucggaagc cucggacuag acugcgaacc ucgaaccggc     1140

cucgacuuca gugaccucua cuaccucaca augaacaaca aacacuggcu aguacacaaa     1200

gaaugguucc acgacauccc ccuacccugg cacgcgggcg cagacacagg cacaccacac     1260

uggaacaaca aagaagcccu gguggaguuc aaggacgccc acgcaaaacg acaaaccguc     1320

gucguacuug ggagucaaga aggcgcagua cacacagcac ucgccggagc acucgaagcc     1380

gaaauggacg gugccaaagg acgccugagc agcggccacc ucaagugcag acuaaagaug     1440

gacaaacuaa gacucaaagg agugagcuac agccuuugca ccgcagccuu caccuucaca     1500

aaaauaccgg ccgaaacccu gcacggaacg gucaccguag aaguccagua cgccggaacu     1560

gacggacccu gcaagguccc agcacaaaug gcgguagaca ugcaaacccu aacaccagua     1620

gggagauuaa uaaccgccaa ccccguaauc acagaaagca cggaaaacag caagaugaug     1680

cuagaacucg acccgcccuu cggggacagc uacauaguaa uaggagucgg agaaaagaag     1740

aucacccacc acuggcaccg aagcgggagc accaucggua aagcguucga agcaacagua     1800

cgcggcgcca aacgcauggc cguacucggu gacaccgccu gggacuucgg cucggucggc     1860

ggggcccuca auagccuugg caaaggcauu caccaaaucu ucggcgccgc auucaaaagc     1920

cuauucggcg gaaugagcug guucagccag auacucauag guacacuucu aauguggcuc     1980

gggcucaaca ccaaaaacgg cucaaucagc cuaaugugcc uggcccucgg aggagugcua     2040

aucuuccuaa gcacagccgu cagcgccgac                                      2070


<210>  2818
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2818
gacacaagcg ucggcaucgu aggacuacua cucacaacag ccauggcagc ugaagucacc       60

agacgcggcu ccgcauacua cauguacuua gaccgcaacg acgccggcga agccaucuca      120

uucccaacaa cacugggaau gaacaaaugc uacauccaaa ucauggaccu cggacacaug      180

ugcgacgcaa cgaugucaua ugagugcccu augcuggacg aaggagucga accagacgac      240

guagacugcu ggugcaacac cacgagcacg ugggucguau acggcacuug ccaccacaag      300

aaaggggaag cccgccgcuc acgccgagcc guaacccuac caucccacuc aacccggaaa      360

cuacaaacac gcucccaaac auggcuagaa agcagagagu acacaaaaca ccugauacgc      420

gucgaaaacu ggauauuccg aaaccccgga uucgcccucg ccgcugccgc cauagccugg      480

cuccugggca gcuccaccuc acagaaaguc aucuaucucg uaaugauccu acucaucgcc      540

cccgcauaca gcauacgaug cauaggcguc ucaaaccgcg acuucgucga aggaaugucc      600

ggaggcaccu ggguugauau cguccuagaa cacgguggau gcguaaccgu cauggcacaa      660

gauaaaccca ccgucgacau cgaacucgua acaaccacag ucucgaacau ggccgaagua      720

cgauccuacu gcuacgaagc cagcaucucg gacauggccu ccgacagccg cugccccacc      780

caaggcgaag cauaccucga caaacaaagc gacacucaau acguaugcaa gcggacacuu      840

guagaccgag gcugggggaa cggcugcgga cucuucggca aaggcagccu uguaacgugc      900

gcaaaguucg ccugcucaaa gaaaaugacc ggaaaaucca uacaacccga aaaccuugaa      960

uaccgaauaa ugcuaagcgu ccacggaucg caacauuccg gaaugaucgu caacgauacc     1020

ggccacgaaa ccgacgaaaa ccgagcaaaa gucgaaauca cacccaacag cccccgcgca     1080

gaagccaccc uagggggcuu cggaagccuc ggacuagacu gcgaaccgcg cacaggccuc     1140

gacuucagcg accuguacua ccucaccaug aacaauaaac acuggcuagu ccauaaagag     1200

ugguuccacg auauccccuu accauggcac gcaggagcag acaccggcac cccacacugg     1260

aacaacaaag aggcacucgu cgaguucaaa gacgcacacg ccaaacgcca aaccgucguc     1320

guacucggcu cacaagaagg cgccguccau acagcccugg cgggagcccu agaagcagaa     1380

auggacggag ccaaaggcag acuaagcucg ggccaccuca aaugccgccu aaaaauggac     1440

aaacucagac ucaaaggagu aagcuacucu uuaugcaccg ccgcguucac auucacaaaa     1500

auucccgcag aaacccucca cggaacagua accguagaag uacaauacgc cggcaccgac     1560

ggccccugca aagucccggc ccagauggcc guugacaugc aaacccuaac acccgucggc     1620

agacuaauaa ccgccaaccc aguaaucacc gaauccacgg aaaacucaaa aaugauguua     1680

gagcuagauc caccguucgg agacagcuac aucgugaucg gcgucggcga aaagaaaaua     1740

acccaccacu ggcaccgcag cggaagcacc auaggcaaag ccuucgaagc caccguccga     1800

ggggcgaaac gcauggcggu ccuaggagac acagcuuggg acuucggcag cguaggcgga     1860

gcgcuaaacu cacucggcaa aggcauccac caaaucuucg gcgcggcauu caaaucguug     1920

uucggaggaa ugucaugguu cucacagaua cucaucggca cacuccugau guggcuaggc     1980

cucaacacca aaaauggcuc aauaagccua augugccuag cacucggcgg cguccuaauc     2040

uuccugagca cagccgucag cgcugac                                         2067


<210>  2819
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2819
acaagcguag gaauugucgg acuccuacua acaaccgcga uggcagcaga aguaacgcga       60

cgcggcucag ccuacuacau guaccuagac cgaaacgacg ccggagaagc caucucauuc      120

ccgaccacac ucgggaugaa uaaauguuac auacaaauua uggaccuagg ccacaugugc      180

gacgcaacca ugucguacga augcccgaug cucgacgagg gagucgaacc ggacgacgua      240

gacugcuggu gcaacaccac guccacaugg gucgucuacg ggaccugcca ccacaagaaa      300

ggagaagcac gacgaagccg acgggcgguu acccuaccaa gccacagcac acgcaaacuc      360

caaacacgca gucaaaccug gcucgaaucc cgagaauaca ccaaacaccu aauacgcguc      420

gagaacugga ucuucaggaa uccaggauuc gcccucgcag cagcagccau cgcauggcuc      480

cugggcucga gcacuagcca aaaaguaaua uacuuaguaa ugauccuacu gaucgccccc      540

gcauacucaa uacgaugcau cggcguaagc aaccgcgacu ucgucgaagg caugucagga      600

ggaacauggg uagacauagu ccucgaacac gggggcugcg ucacgguaau ggcacaggac      660

aaaccaacag ucgacauaga gcuagugacc accacaguca guaacauggc ggaaguccga      720

ucguacugcu acgaagccag cauaucagac auggccucag acucacgcug ccccacacag      780

ggagaggccu accucgacaa gcaaagcgac acacaauaug ucugcaaaag aacauuaguc      840

gaucgcgggu ggggcaacgg gugcggccuc uucggcaaag gcagucucgu aacaugcgca      900

aaauuugcau gcuccaagaa aaugacagga aaauccauac aacccgaaaa ucucgaguac      960

agaaucaugc uauccgucca cggcucccaa cauagcggga ugauagugaa cgacacgggc     1020

cacgaaacag acgaaaaccg agccaaaguc gaaauaacac ccaacagucc ccgcgcagaa     1080

gcaacacuag gcggguucgg cagccuaggc uuagacugcg aaccacgcac aggcuuagac     1140

uucagcgacc uauacuaccu caccaugaac aacaaacacu ggcuggucca caaagagugg     1200

uuccacgaca ucccacuccc cuggcaugca ggagcugaca cgggcacccc acacuggaac     1260

aacaaagagg cucucguaga guucaaagac gcacacgcaa aacgacaaac agucgugguc     1320

cuaggcucac aagaaggagc gguacauacc gcacuagccg gagcccucga ggccgaaaug     1380

gacggagcga aggggcgccu aagcucagga caccuaaagu gccgacuuaa aauggacaaa     1440

cuacgauuga aaggagucuc cuacucccuc ugcacagcag ccuuuacauu uaccaaaaua     1500

ccggccgaga cccugcacgg gacuguaaca guugagguac aauacgccgg gaccgacggc     1560

ccaugcaaag uaccagccca aauggcagua gacaugcaga cccuaacccc cguagggcgc     1620

cugauaaccg ccaauccagu caucacagaa ucaacggaaa acucaaaaau gaugcuagaa     1680

cuagaucccc cauucggcga cagcuacaua gucauaggag ucggagaaaa gaaaauaacc     1740

caccacuggc acaggucagg cucaacaauc ggaaaagcau ucgaagccac gguacgaggc     1800

gcgaaaagaa uggcgguccu cggcgacacu gcaugggacu ucggauccgu aggcggcgcc     1860

cucaacucac ucggaaaagg aauccaccaa aucuucggcg ccgcauucaa aucacuguuc     1920

ggcgggaugu ccugguucag ccaaauacuc aucggaacgc uacuaaugug gcuaggccua     1980

aacaccaaga acgggagcau cucccugaug ugccucgccc uaggaggagu acugauauuc     2040

cugagcacag ccguaagcgc cgac                                            2064


<210>  2820
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2820
accuccgucg gcaucguagg acuccuccua acaaccgcca uggcagccgc aguuacacga       60

cgaggaagcg ccuacuacau guaccuagac agaaacgacg ccggagaagc cauauccuuc      120

ccgacgaccu uaggcaugaa caaaugcuac auacaaauaa uggaccuagg acacaugugc      180

gacgcaacga ugucauauga augccccaug cucgacgaag gaguggaacc ugacgacgua      240

gacugcuggu gcaacacgac aucaaccugg guaguauacg gaaccugcca ccacaagaaa      300

ggcgaagccc gacguucccg gcgagccgua acccucccca gccacuccac acgcaaacua      360

cagacacgau cacaaaccug gcuagaguca cgagaauaca caaaacaccu gauacgcgua      420

gagaacugga ucuuccgcaa ccccggauuc gcacuagccg ccgcugccau cgcauggcua      480

cuaggcagcu cgaccucaca aaaaguaaua uaccucguaa ugauccuccu aaucgcccca      540

gccuacucca uacgcugcau cggggucuca aaccgcgacu uugucgaagg cauguccgga      600

ggaaccuggg ucgacauagu acucgaacac ggcggcugug uaaccguaau ggcacaagac      660

aagccuacag uagacaucga gcuagucaca acaacaguau caaacauggc cgaaguacgc      720

uccuacugcu acgaagcaag cauaagcgac auggcaagcg acagccggug ccccacacaa      780

ggcgaagccu aucuugacaa acagagcgac acacaauacg ucugcaaaag gacccucgua      840

gacagaggcu ggggcaacgg cugcggacua uucggaaaag ggucacucgu cacaugcgca      900

aaauucgccu gcaguaagaa gaugaccggg aaaagcaucc aacccgaaaa ccuagaauac      960

cguaucaugc ucagcguaca cggcucacaa cacuccggca ugauugucaa ugacaccggu     1020

cacgaaacag acgagaaccg cgcaaaagug gaaauaaccc ccaacucgcc cagggccgaa     1080

gcgacacucg gaggauucgg aucccuuggc cuagacugcg aacccaggac aggacucgac     1140

uucucagacc ucuacuaccu uaccaugaac aacaaacacu ggcucguaca caaagaaugg     1200

uuucacgaca ucccccuacc auggcacgcg ggagccgaca caggcacacc acacuggaac     1260

aacaaagaag cccuagucga auuuaaggac gcacacgcaa aaagacaaac cguaguuguc     1320

cuaggaucac aagaaggcgc aguacacacg gcccucgccg gagcccucga agccgaaaug     1380

gacggcgcca aaggccgacu aagcagcggc caccucaaau gccgccugaa aauggacaaa     1440

cuccgacuaa aaggugucag uuauucccua ugcacagccg ccuuuaccuu cacaaaaaua     1500

cccgcagaaa cccuccacgg caccgucacc gucgaggucc aauacgccgg cacggacggc     1560

cccugcaagg uacccgcaca aauggccgug gacaugcaaa cccugacccc cgugggcaga     1620

cucauaacag caaacccagu cauaacagaa agcacagaaa acuccaaaau gaugcucgaa     1680

cuagacccgc ccuucgggga cucauacaua guaauaggcg uaggagagaa gaaaaucacc     1740

caccacuggc accgaucggg aucuaccauc ggcaaagcau ucgaagccac cgugcgaggc     1800

gccaaacgaa uggcaguccu cggcgacacc gcaugggacu uuggcucugu cggaggagcc     1860

cuaaacagcc ucggcaaagg cauccaccaa aucuucggug cagcguucaa aagccucuuc     1920

ggcggcaugu ccugguucag ccaaauccug aucgggaccc uacucaugug gcuaggccuc     1980

aauaccaaaa acggauccau cagccuaaug ugccuggccc uaggcggagu ccugaucuuc     2040

cucucaacag cagucagcgc agac                                            2064


<210>  2821
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2821
cgaggcgcgg acacgucagu cggcauaguc ggucuacuac uuacaaccgc cauggcagcc       60

gccguuacac gccggggauc cgccuacuac auguaccucg accgcaacga cgccggcgaa      120

gcaaucucgu ucccaaccac acuaggcaug aacaaaugcu acauccaaau uauggaccua      180

ggacacaugu gcgacgccac caugagcuac gagugcccaa ugcuagacga aggaguggag      240

ccagacgacg uggacugcug gugcaacacu acaucgaccu gggucgucua cgggaccugc      300

caccacaaga aaggagaagc acgaagguca agacgagcug uaacacuacc aagccacucc      360

acaaggaaau uacagacccg aagccaaaca uggcucgaau ccagggaaua caccaaacau      420

cucauucgcg uagaaaacug gauauuccgc aacccaggcu ucgcacuagc ggcggccgcg      480

auagcuuggc uccucgggag cuccaccucg caaaaaguaa ucuaccuagu caugauccua      540

cucauagcuc ccgcguacag caucagaugc aucggcguaa gcaaccgcga cuucgucgaa      600

ggcaugagcg gcggcaccug gguagacaua guacucgaac acgggggcug ugucaccgua      660

auggcacaag acaaaccaac gguggauauc gagcuaguca caacgaccgu cuccaacaug      720

gccgaaguaa gaagcuauug cuacgaggca uccauaagcg acauggcaag cgacucgcgc      780

ugcccaacgc aaggugaagc auaccuagac aaacaaagcg acacacaaua cguaugcaaa      840

cgcacccucg ucgacagagg guggggcaau ggaugcggac ucuucggcaa aggcucccuc      900

gucacaugcg caaaauucgc cugcuccaaa aagaugacag gcaaaucaau ccaacccgaa      960

aaccuagagu accgaauaau gcucagcguc cacggaagcc aacacagcgg aaugauaguc     1020

aacgacacag gacacgaaac cgacgaaaac cgcgcaaaag ucgaaaucac accaaacagu     1080

ccaagagcgg aagcaacacu aggcggauuc ggaucacuag gacuagauug cgagccacgc     1140

acaggacuag acuucagcga ccuauacuac cugacgauga acaacaaaca cuggcucgua     1200

cacaaagagu gguuccacga caucccccuc ccauggcacg ccggcgccga caccggcacc     1260

ccgcauugga acaacaaaga agcccuagua gaguucaaag acgcacacgc aaagcgccaa     1320

accgucgucg uacuaggaag ccaagaagga gcgguccaca cagcccuagc cggcgcccua     1380

gaagcugaaa uggacggagc aaaaggaaga cuaagcagcg gucaccucaa augcagacuc     1440

aaaauggaca aacuacgccu caaaggcgua ucguacuccc ucugcaccgc cgccuucaca     1500

uucaccaaaa uaccagcaga gacacuacac ggaaccguga caguggaagu ccaauacgcc     1560

ggcaccgaug gaccaugcaa aguacccgca caaauggcag uagacaugca aacccugaca     1620

cccgucggca gacuaaucac agccaaccca gucauaaccg aaagcaccga gaacagcaag     1680

augaugcucg agcucgaccc gccauucggg gacagcuaca ucgucauagg agugggagaa     1740

aagaaaauca cccaccacug gcaccgaagc ggcucaacaa uaggaaaagc guucgaagcg     1800

accguccgag gagccaaacg cauggcagua cucggugaca cagccuggga cuucggcuca     1860

guaggcggcg cccuaaacuc ucuaggaaaa ggcauacacc aaaucuuugg cgcagccuuc     1920

aaaucacucu ucggcggcau gagcugguuc ucccagaucc uaauaggcac acuacuaaug     1980

uggcucggac ucaacaccaa aaauggcucg aucagccuga ugugccucgc acuaggcggc     2040

guacugaucu uccuauccac agcgguaagc gccgac                               2076


<210>  2822
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2822
cgcggccuau cgucacggaa aagacggagc cacgacgugc uaacagugca auuccucaua       60

cucggcaugc uacuaaugac cggaggcguc acccgccgag gcucagccua cuacauguac      120

cuagacagga acgacgccgg ggaagccaua aguuucccca cgacacuggg caugaacaaa      180

ugcuauauac aaaucaugga ucuaggccac augugcgacg ccaccaugag cuacgaaugc      240

ccgaugcuag acgaaggagu cgagccagac gacguggacu guuggugcaa cacaacaucc      300

acauggguag ucuacggaac augccaccau aagaaaggag aagcccgacg cucaaggaga      360

gccgugaccc ucccaagcca cucgaccaga aaacuccaaa caagaagcca aacguggcuc      420

gaaucgaggg aauacacgaa gcaccuaaua agagucgaaa acuggauauu ccgaaaccca      480

ggauucgcac uggccgcggc cgccaucgcc uggcuacucg gaagcagcac gagccaaaaa      540

guaauuuauc uuguaaugau cuugcugaua gccccggccu acagcauccg augcaucggc      600

guuucgaaua gagacuucgu agaaggcaug agcggcggua ccuggguaga uaucguccuc      660

gaacacgggg gaugcgugac cguaauggcc caggacaaac cgaccgucga cauagagcua      720

guaaccacaa cagucagcaa cauggcagaa gucaggaguu acugcuauga agcaucaaua      780

ucagacaugg caagcgacag cagaugcccu acacaagggg aggcauaccu cgacaaacaa      840

agugacaccc aauacgucug uaaaagaacc cuagucgacc gcggaugggg gaauggcugc      900

ggacuauucg gaaaagggag ccuagugaca ugcgccaaau ucgcaugcuc aaagaaaaug      960

acaggaaaaa gcauccaacc agaaaaccuc gaguacagaa uaaugcucag cguccacggg     1020

ucacagcaca gcggcaugau aguaaacgac accggacacg aaaccgacga aaaccgagcc     1080

aaaguagaaa uaaccccuaa uagccccaga gcugaggcaa cccuaggagg cuucggcagc     1140

uugggacucg acugcgaacc ccgaacggga cucgacuucu ccgaccuaua cuaccucacc     1200

augaacaaca aacacuggcu cgugcacaaa gaaugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacacagg aacgccucac uggaacaaca aggaagcccu uguagaguuc     1320

aaagacgcgc acgcaaaacg ccaaacggug gucgugcucg gaagucaaga aggagccgug     1380

cacacggcac uagccggcgc gcucgaagcc gaaauggacg gagcaaaggg gcgacugagc     1440

agcggacacc ugaaaugccg gcugaaaaug gacaaacuga gacuaaaagg agucagcuac     1500

agccuaugca ccgccgcauu cacauucaca aaaauaccgg ccgaaacacu acacgguaca     1560

gucacgguag aaguccaaua cgcggggaca gacggacccu gcaaaguccc agcgcaaaug     1620

gcgguagaua ugcagacacu cacacccgug gguagauuaa uaacagcaaa ccccgucauc     1680

acagaaagca cggaaaacag caaaaugaug cuagaacuag acccacccuu cggagauuca     1740

uacauaguca uaggcguagg cgaaaagaaa auaacccacc acuggcaccg cagcggcagc     1800

acaaucggaa aagcauucga agcaacggug aggggagcca aacgaauggc gguccucgga     1860

gauacagccu gggacuucgg aagugucggg ggugcacuga acagccuagg caaaggcaua     1920

caccaaaucu ucggagcagc guucaaaagc cucuucgggg gaaugagcug guucucccaa     1980

auauugauag gaacucuacu cauguggcuc gggcuaaaca cuaaaaaugg cagcaucagc     2040

cucaugugcc uggcccucgg aggcguccuc auauuccuaa gcacagccgu cagcgccgac     2100


<210>  2823
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2823
cgaggccuua gcucccgcaa acgacgcagc cacgacguac uaaccgucca auuccuaaua       60

cuaggcaugc uccucaugac aggcgccgua acaagacggg guuccgcaua cuacauguac      120

cucgacagga acgacgcggg agaagccauu uccuucccaa ccacacucgg caugaacaaa      180

ugcuacauac aaauaaugga ucucggacac augugcgacg ccaccauguc auacgaaugc      240

ccaaugcuag acgaaggagu ggaacccgac gacguagacu gcuggugcaa caccacaucc      300

accuggguag ucuacggaac gugccaccac aagaaaggag aagcacggcg aagccgacga      360

gccguaaccc uccccuccca cagcacucgc aaacuccaaa cacgaucaca aacauggcua      420

gaaucccgcg aauacaccaa acaccuaaua cgcgucgaaa acuggaucuu ucgaaaccca      480

ggcuucgccc ucgcagcagc cgcgauagca uggcuucuug gaagcagcac aagucaaaaa      540

guaaucuacu uaguaaugau ccugcuuaua gcgccugcau auucgauacg gugcaucggg      600

guaagcaacc gcgacuucgu cgaaggaaug ucaggaggca ccuggguaga cauuguacuc      660

gaacacggag gaugcguaac cguaauggcc caagacaaac cuacagucga cauagagcuc      720

guaacgacca caguaagcaa cauggcagag guccgaucau acugcuacga agccucaaua      780

agugacaugg ccucagacuc acgcugccca acccaaggcg aggccuaccu agacaagcaa      840

uccgacacac aguacgucug caaacgcaca cucgucgaca gagggugggg caacgguugc      900

ggccuuuucg gcaaaggauc acucguaaca ugcgcaaaau ucgccugcuc caagaaaaug      960

acuggaaaaa gcauacaacc agaaaaccua gaguacagaa uaaugcuauc cguacacggg     1020

agccagcacu caggaaugau cgucaacgac acgggacacg aaaccgacga aaaccgcgca     1080

aaaguagaaa uaacacccaa cucaccccga gcagaagcga cccuaggagg auucggaucc     1140

cucggacuag acugugaacc cagaacaggc cucgacuuca gcgaccucua cuaccucacc     1200

augaacaaca aacacuggcu uguccacaaa gagugguucc acgacauccc ccuacccugg     1260

cacgccggag cagacaccgg caccccacac uggaacaaca aagaagcccu cgucgaguuc     1320

aaagacgcgc acgcaaaacg acaaaccguc gucguucuug gaucccagga aggagccguc     1380

cauacagcac uagcaggcgc ccuggaagcc gaaauggacg gcgccaaggg gcggcuauca     1440

ucaggccacc uaaaaugucg ccuaaagaug gacaaacucc gacuaaaagg ggucucauac     1500

ucucuaugca ccgcggcauu caccuucacc aagauccccg cagaaacccu acacggaacc     1560

gucacaguag aaguacaaua cgccggcaca gacggccccu gcaagguccc agcccaaaug     1620

gccguagaca ugcaaacacu gaccccagua ggacgacuaa uaaccgcgaa ccccguaaua     1680

accgaaucga ccgaaaacuc aaaaaugaug cucgaacuag acccaccguu uggcgacagc     1740

uacaucguca ucggcgucgg agagaaaaag auaacccacc acuggcacag guccggaagc     1800

accaucggua aggcuuucga agcaaccguc cgcggagcca aacgcauggc cguccuagga     1860

gacacagcau gggauuucgg cagcgucggc ggagcccuga acagccucgg aaaaggaaua     1920

caccaaauau ucggagccgc guucaaaagc cuauucgggg gcauguccug guucucacaa     1980

auccuaaucg gcacacuacu gauguggcuc ggccucaaca ccaaaaacgg gagcauuagc     2040

cucauguguc uagcucuagg cggcguacua aucuuccuau ccacagcagu gagcgcggac     2100


<210>  2824
<211>  2106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2824
cgcaucauaa acgccagaaa agaaaagaaa cgaaggggag ccgacacauc aguaggcauc       60

gucggacuac ucuugaccac cgcaauggcc gcggaaguca ccagaagggg cucagcauac      120

uacauguacc uagaccguaa cgacgccgga gaagcaaucu ccuuccccac cacccuugga      180

augaacaaau gcuacauaca aauaauggau cuaggccaua ugugcgacgc caccaugucc      240

uacgaaugcc ccaugcucga cgaaggagua gagcccgacg acguggacug uugguguaau      300

accaccagca caugggucgu cuaugggacc ugccaccaca agaaaggcga agcacgaaga      360

agucgacgag caguaacacu accgagccac agcacccgaa aacuacaaac aagaagucaa      420

acauggcuag aaagccgaga auacacgaaa caccuaauaa gaguagagaa cuggauauuc      480

agaaacccag gguucgcccu cgccgccgcg gccaucgcau ggcugcuagg cuccagcaca      540

ucgcaaaaag uaauauaccu agucaugauu cuacuaaucg cacccgcaua cagcauccgc      600

ugcauaggcg ucucgaaccg cgacuucguc gagggcaugu caggaggcac auggguggac      660

aucgucuuag aacacggugg augcgucacc gucauggccc aagacaaacc caccguagac      720

aucgagcugg uaaccaccac cguauccaac auggccgaag ugcgguccua cugcuacgaa      780

gccucaauaa gcgacauggc cuccgacagc agaugcccaa cccaagggga ggcguaucuc      840

gacaaacaaa gcgacaccca auacgucugc aaacgaacac ucguagaccg cgggugggga      900

aacggcugcg gacucuucgg aaaaggcagc cucguaaccu gugccaaauu cgcaugcucg      960

aagaaaauga ccggaaaauc cauacaacca gagaacuuag aauaccgcau caugcuaagc     1020

guacauggca gccaacacag cggcaugaua guaaacgaca caggccauga aacagacgaa     1080

aaccgagcaa aagucgaaau cacccccaac agcccgagag cagaagccac acuaggagga     1140

uucggcagcc uaggccugga cugcgaacca aggacaggcc ucgacuucag cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcua guacacaaag aaugguucca cgacauacca     1260

cuccccuggc acgcaggcgc cgacacaggg acaccccauu ggaauaacaa agaagcacug     1320

guagaguuca aagacgccca cgccaagaga caaaccguag uaguacuagg cucccaggaa     1380

ggggcagucc acacagcacu agcgggagca cuggaagcag aaauggacgg cgccaaaggg     1440

cgacuaagca gcggccaccu gaaaugcaga cuuaaaaugg auaaacuacg acuaaagggc     1500

gucagcuaca gccucugcac cgcugccuuc acauucacga aaauccccgc cgaaaccuua     1560

cacggcacgg ucacagucga aguacaauac gccggaaccg acgguccaug caaaguaccc     1620

gcacaaaugg ccgucgacau gcagacacua acacccgucg gacggcuaau aacagccaac     1680

cccguuauca cagaaucgac cgaaaauuca aaaaugaugc ucgaacuaga cccaccguuu     1740

ggagacuccu acaucguaau cggcgucgga gaaaagaaaa ucacacacca cuggcaccgc     1800

agcggcucca cgauagggaa agcauucgag gccaccgucc gaggagcaaa aagaauggcc     1860

guccucggcg acacagcaug ggacuucggc agcgucggag gcgcgcucaa cagucucgga     1920

aaaggcaucc accaaauauu cggcgcagca uucaaaagcc uuuucggcgg caugagcugg     1980

uuuucacaaa uccuaauagg aacccuccuc auguggcucg gccuaaacac caaaaacggg     2040

ucaauaagcc uaaugugccu ggcccucggc gggguccuaa uauuccucuc cacggcaguc     2100

agcgca                                                                2106


<210>  2825
<211>  2100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2825
auaaacgccc gaaaagagaa aaagcgccga ggagccgaca cauccguagg uauagucggc       60

cucuuauuaa caacagcaau ggcugccgaa guaacaagac gcggcagcgc auauuacaug      120

uaccuugacc gaaacgaugc aggggaagca auaucauuuc caacaacacu uggcaugaac      180

aaaugcuaua uccaaaucau ggacuuggga cacaugugcg acgccacaau gucauacgaa      240

ugcccgaugc uagacgaagg gguggaaccg gacgacguug acugcuggug caacacaacu      300

ucaaccuggg uagucuacgg aaccugccac cacaagaaag gcgaagcaag aagauccaga      360

cgggccguca cacuccccag ccacagcacu agaaaacuac aaacccgaag ccaaacaugg      420

cucgaaagcc gcgaauacac aaagcaucug auccgagugg aaaacuggau cuuccgaaac      480

cccggcuucg cccucgccgc cgcagccaua gcauggcucc ugggauccuc cacgucacaa      540

aaagugauau accuaguaau gauccugcuc auagccccgg ccuacuccau acgcugcaua      600

ggcguaagca accgagacuu cguagaggga augucagggg gcaccugggu cgacaucguc      660

cuagaacacg ggggcugcgu cacagucaug gcgcaagaca agcccacagu cgacaucgag      720

cucgucacaa caaccguauc aaacauggca gagguaaggu ccuacugcua cgaagcguca      780

aucucagaca uggccagcga cucccguugc cccacacaag gcgaggccua ccuagacaaa      840

caauccgaca cacaauacgu augcaaacga acacucguag accggggcug gggaaacggc      900

ugcggacucu ucggcaaagg cagccucguc acaugcgcga aauucgccug cucaaagaaa      960

augaccggaa aaagcaucca acccgagaac cuagaauacc gaauaaugcu aagcguacac     1020

gggucccaac acuccggaau gaucgucaac gacaccggac acgaaaccga cgaaaacaga     1080

gcuaaaguag aaaucacgcc aaacucgcca cgcgcagaag ccacgcucgg aggauucgga     1140

ucacuagggc uagacugcga gccacgcacc gggcuagacu ucuccgaccu auacuaccua     1200

accaugaaca acaaacacug gcuuguucac aaagaauggu uccaugacau cccccucccc     1260

uggcacgcag gagccgacac aggaacccca cacuggaaca acaaagaagc ccuaguagag     1320

uucaaagacg cacaugcaaa acgccagacc gucgucguac uaggauccca agaaggagcu     1380

guccauaccg cacucgccgg cgcccucgaa gccgaaaugg acggagccaa agggcgccua     1440

agcuccggac accucaaaug ccgacuaaaa auggacaaac uaagacugaa aggcgucagc     1500

uacagccuau gcacagccgc auucacauuc accaaaauuc cagcagaaac gcuccacgga     1560

acagucaccg uagaaguaca auacgccggc acagacggcc ccugcaaagu gccagcccaa     1620

auggcgguag acaugcaaac acucacacca gucggccgcc uaauaacggc uaacccagua     1680

aucaccgaaa gcaccgaaaa uagcaaaaug augcuugaac ucgacccgcc cuucggagac     1740

agcuacaucg ucaucggcgu cggggaaaag aaaauaacac accacuggca ccgaagcggg     1800

uccacaaucg gcaaagccuu cgaagcaacc guaaggggcg caaaacgcau ggccguacuc     1860

ggagacacgg ccugggacuu cgguuccguc ggaggcgcac uaaacagccu gggcaaaggc     1920

auccaucaaa ucuucggagc agcauucaaa ucacuuuuug ggggaaugag cugguucucc     1980

caaauccuga ucggcaccuu acuaaugugg cugggccuua acaccaaaaa uggcagcauc     2040

ucgcucaugu gccucgcacu cggaggaguc cuaaucuucc ugagcacagc cguauccgcc     2100


<210>  2826
<211>  2103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2826
auaaucaacg caagaaagga aaagaaacgc cgcggugccg acacaucagu aggcauaguc       60

ggccuccucc ucacgacagc uauggccgca gaaguaacac gacgcggaag cgcguacuac      120

auguaccucg accgcaacga cgccggagaa gcgaucuccu ucccgaccac acucggcaug      180

aacaaaugcu acauccaaau cauggaucuc ggacacaugu gcgacgccac cauguccuac      240

gagugcccaa ugcuagacga aggagucgaa cccgacgacg uagauugcug gugcaacacg      300

accuccacau gggucguuua cggaaccugu caccacaaga agggcgaagc ccgacgaagc      360

cgcagagcag ucacccuacc cagccacucc acucgcaaac uccagacacg aagccaaaca      420

uggcucgaga gccgagagua cacaaaacac cucauaaggg uagaaaacug gaucuuccga      480

aauccaggcu ucgcccuagc cgcagccgcc aucgcguggc uucuaggcuc aagcacguca      540

caaaagguca ucuaccucgu caugauccua cuaaucgcuc cagccuauuc aauccggugc      600

auagguguaa gcaaccgaga cuucguagaa ggcaugucag gaggaacaug gguagacauc      660

guccuggaac auggcggaug cguaaccguc auggcccaag acaaacccac aguagacauc      720

gaacucguga ccaccaccgu aagcaacaug gcagaagucc gaagcuacug cuacgaggcu      780

ucaaucagcg acauggcauc agacagcaga ugccccacac aaggcgaagc cuaccuagac      840

aagcagucag acacccaaua uguaugcaaa cgcacccuag ucgauagggg auggggaaac      900

ggaugcggac ucuucgguaa aggcagucuc gugaccugcg cgaaauucgc gugcuccaag      960

aaaaugacgg gcaaaagcau ucaacccgaa aaccuagaau acagaaucau gcuaucaguc     1020

cacggcuccc aacacuccgg caugauuguc aacgacaccg gccacgaaac cgacgaaaac     1080

cgagcaaaag ucgaaaucac acccaacagc ccgcgcgccg aagccacacu cggcggcuuc     1140

ggcagccugg gccucgacug ugaaccccgc acaggacuag acuucagcga ccucuacuac     1200

cugacaauga acaacaaaca cuggcucguc cacaaagaau gguuccacga cauuccccuc     1260

cccuggcacg ccggcgcaga uaccggaacc ccacacugga acaauaaaga agcacucgua     1320

gaguucaagg acgcgcacgc aaaacggcaa acagucgugg uccugggcuc gcaagaaggc     1380

gcaguccaca cagcccucgc cggcgcccua gaagccgaaa uggacggagc aaaagguaga     1440

cucagcucag gacaccuaaa augcagacuc aagauggaca aauuacgccu caaaggcgua     1500

ucuuacuccc uaugcaccgc cgcauucacc uuuaccaaaa ucccggcaga aacgcuccau     1560

ggaacaguca ccgucgaggu ccaauacgcc ggaaccgaug gaccuugcaa aguccccgcc     1620

caaauggcgg uugacaugca gacccugacc cccgucggcc gacucaucac cgccaaccca     1680

gucaucacug aaagcacaga aaacuccaaa augaugcucg aacucgaccc acccuucggc     1740

gacaguuaca uugucaucgg cgucggggaa aagaagauca cacaccauug gcaccggucc     1800

ggaagcacga uuggaaaggc cuucgaagca accgucagag gagccaaaag aauggcggua     1860

cucggcgaca ccgcauggga cuuuggcucc gucggcggag cacuuaacuc acucggaaaa     1920

ggcauacacc aaauauucgg ugcugccuuc aaaucacucu ucggcggcau gagcugguuc     1980

ucacagauac uaaucggaac acuacuaaug uggcuaggcc ucaacaccaa aaacggaagc     2040

auaucacuua ugugccuagc ccucggcgga guacuaaucu ucuuguccac agcagucucc     2100

gcc                                                                   2103


<210>  2827
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2827
aacgccagaa aagaaaagaa acgaagaggc gccgacacca gcguuggaau cgucggacua       60

cuacuaacca ccgcgauggc agccgaaguc acgagaaggg gaagcgccua cuacauguac      120

cuagacagaa acgacgcagg cgaggcaaua aguuucccca caacgcuagg aaugaauaaa      180

uguuacauac aaauaaugga ucucggacac augugcgacg caacaaugag cuacgaaugc      240

ccaaugcuag acgaaggagu cgaaccagac gauguggacu gcuggugcaa cacaacuagc      300

acaugggucg ucuacggcac augccaccac aagaaaggag aagcacgaag gagucgcaga      360

gccgucacgc uacccagcca cucaacgaga aaacuccaga ccaggagcca aacguggcua      420

gagagcagag aauacacaaa acaccuaaua agagucgaaa acuggaucuu ccgaaaccca      480

ggcuucgccc ucgccgccgc agccauagcc uggcuccuag guucuaguac cagccaaaaa      540

guaauauacc uagugaugau ccuacugaua gcacccgcau acuccauccg uugcauagga      600

gucagcaacc gcgacuucgu agaaggaaug agcggaggaa ccugggucga uaucguacua      660

gaacacggcg gcugcgucac agucauggcc caagacaaac caaccgucga cauagaacuc      720

gugacaacaa caguuagcaa cauggcggaa gugcgaagcu acugcuauga agccuccaua      780

ucagacaugg caucggacag ccgaugucca acacaaggag aagccuaccu agacaagcaa      840

uccgacacac aauacguaug caaaagaacc cuagucgauc gaggcugggg caacgggugc      900

ggccuuuucg gaaaaggaag ccuggucaca ugcgccaaau ucgcgugcag caagaaaaug      960

acaggcaaau caauccaacc agaaaaccuc gaauaucgaa ucaugcucuc gguacacggc     1020

agccaacacu ccggaaugau cguaaacgac acaggucacg agacagacga aaacagagca     1080

aaagucgaaa ucacgcccaa cagcccaaga gccgaagcaa cacucggcgg guucgggagc     1140

cuaggacuag acugcgaacc ccgaacaggc cucgacuucu cggaccucua cuaucuuacc     1200

augaacaaca aacacuggcu aguccacaaa gaaugguucc acgacauccc ccuccccugg     1260

cacgcagggg cagacaccgg aacgccgcau uggaacaaua aagaggcucu agucgaguuc     1320

aaggacgcac acgcgaaaag acaaaccguc gucguauuag gcucacaaga gggcgccguc     1380

cacacagcgc ucgcaggggc acuagaagcc gagauggacg gcgcgaaagg aaggcuauca     1440

ucaggucacc ucaaaugccg gcuuaagaug gacaaacuga gacuaaaagg gguauccuau     1500

ucacucugca cagcagcauu cacauucaca aaaauccccg cagaaacacu gcacggaaca     1560

guaaccguag aaguccaaua cgcaggcacc gacggcccau gcaaaguacc ugcccaaaug     1620

gccguagaca ugcagacccu aacgcccguc ggccggcuca ucacggccaa cccgguaaua     1680

acugaaucaa cagaaaacuc uaaaaugaug cuggaacugg acccacccuu cggcgacagu     1740

uacauaguca ucggugucgg ugaaaagaaa aucacccacc acuggcacag aagcggaagc     1800

acuauaggca aagcguucga agccacagua agaggcgcaa aaaggauggc aguacuagga     1860

gacaccgccu gggacuucgg cucaguagga ggagcacuaa acucgcucgg caagggaauc     1920

caccaaauau ucggagccgc auucaagagc cuauucggcg gaaugagcug guucucacaa     1980

auccuaaucg gaacgcugcu aauguggcuc ggccuaaaca caaagaacgg cagcaucagc     2040

cuaaugugcc ucgcccucgg gggcguccuu auauucuuga gcacagcagu aagcgcc        2097


<210>  2828
<211>  2094
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2828
gccaggaaag agaaaaagag aagaggcgcg gacacaucag uaggaauagu cggacuacug       60

cuaacaaccg ccauggcagc agaaguaacc cgaagaggcu cagccuacua cauguaccuc      120

gaccgaaacg acgccggaga agccauauca uuccccacca cccucggaau gaacaaaugc      180

uacauccaga ucauggaccu cggccacaug ugcgaugcaa ccaugagcua cgaaugccca      240

augcucgacg aaggcguaga accagacgac guagacugcu ggugcaacac aaccaguacg      300

uggguagucu auggcacaug ccaccacaag aaaggcgaag cacgcagaag ccgccgcgca      360

gucacucucc caucucacag caccagaaaa cuccagacaa ggagccagac cuggcuggaa      420

ucccgagaau acaccaaaca ccuaauaagg gucgaaaacu ggauuuuccg caacccaggc      480

uucgcacucg cagccgcagc cauagccugg cuacugggca gcagcacaag ccaaaaagug      540

aucuaucucg ucaugauacu ccugauugcc ccagcuuacu ccaucagaug caucggcgua      600

agcaaccgag acuucgucga ggggaugucg ggaggaaccu ggguugacau cguacucgaa      660

cacggcggau gcguaaccgu aauggcucaa gacaaaccca ccguugacau agaacuggug      720

acaaccacag uauccaacau ggcagaggug agaucauacu gcuacgaggc uucaaucucu      780

gacauggccu cggacagccg cugccccaca cagggggaag cguaccucga caaacaauca      840

gacacacagu acgucugcaa acgaacccuc guagaccgcg gauggggcaa cgggugcggg      900

cuauucggca aaggaucacu cgucacaugc gcaaaauucg cuugcucgaa gaaaaugaca      960

ggcaaaagca uacaaccaga gaaccucgaa uacagaauaa ugcugucagu acacggcuca     1020

caacacagcg gaaugauugu caacgacaca ggacacgaga ccgacgaaaa cagggccaaa     1080

guagaaauaa cccccaacag cccccgagcc gaggcaacac uuggaggauu cggcucccua     1140

ggauuagacu gcgaaccgcg caccggacug gacuuuagcg accuauacua ccucacaaug     1200

aacaacaaac acuggcuagu ccacaaagag ugguuccacg acauaccccu cccauggcau     1260

gccggagcag acacaggaac cccacacugg aacaacaaag aagcgcuggu agaguucaaa     1320

gacgcccacg caaaacgcca aacagucgug guccucggca gccaagaagg cgccguccac     1380

accgcacucg ccggcgcacu agaagcagaa auggacggcg cgaaaggccg gcucagcucc     1440

gggcaccuaa aauguagacu aaaaauggau aagcuacgcc uaaaaggggu aucauacagc     1500

cuaugcaccg ccgccuucac auucacaaaa auaccggcag aaacacugca cggaacagua     1560

acagucgaag uacaauacgc aggaaccgau ggcccaugca aaguuccagc ccaaauggca     1620

gucgacaugc aaacacugac cccggucgga agacuuauca cagcaaaccc cgucaucaca     1680

gaaucaaccg aaaacagcaa aaugaugcuc gaacuagacc caccauucgg cgacaguuac     1740

auagugauag gcguaggaga gaagaaaauc acccaucacu ggcacagauc cgggagcacu     1800

auagggaaag ccuucgaggc cacaguaagg ggagccaaac gcauggccgu ccucggugac     1860

acagcauggg acuucggcuc aguaggcggc gcccucaacu cccucggaaa aggaauccac     1920

caaauauucg gggccgcauu caagagccug uucgggggca ugagcugguu cucccaaauc     1980

cuaauaggca cccuccucau guggcuaggc cucaauacca aaaacggaag uaucucacuc     2040

augugccuag cacuaggcgg cguccucaua uuccucagca cagccguuag cgcg           2094


<210>  2829
<211>  2091
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2829
cgaaaagaga agaaaagacg cggagcagac accagcgucg gaaucgucgg ccuucuccuc       60

acuacagcca uggccgccga agucacgaga cgcggauccg cauacuacau guaucuagac      120

cgaaacgacg ccggcgaggc aaucucauuc cccaccaccc ucggcaugaa uaaaugcuau      180

auccagauaa uggaccucgg ccacaugugc gacgcaacaa ugagcuacga augccccaug      240

cucgacgagg gaguagaacc cgaugacguu gacugcuggu gcaacacaac cucgaccugg      300

gucguguacg gcacaugcca ccacaagaaa ggagaagcga gacgcucccg ucgagcagua      360

acccuuccau cccacagcac ccggaaacuc caaacacgcu cccaaaccug gcucgaaucc      420

cgcgaauaua caaaacaucu aauccgagua gaaaacugga ucuuccgcaa cccgggauuc      480

gcccuggcag cggccgccau cgccuggcua cucgguagcu ccaccagcca aaaaguaauc      540

uaccucguua ugauacuacu aaucgcccca gcauacucaa uacgcugcau cggcguaagc      600

aaccgagacu ucgucgaagg aaugucaggc ggcaccuggg ucgacaucgu ccuagaacac      660

ggcggaugcg ucaccgucau ggcgcaagac aaaccaaccg uagacauaga gcuaguaaca      720

accaccguau caaacauggc cgaaguccga agcuacugcu acgaagcauc gauauccgac      780

auggccuccg acucacgcug ccccacccaa ggagaagccu accuagacaa acagucggau      840

acgcaauacg ucugcaaacg aacccuaguc gaccgaggcu ggggaaacgg cugcggccuc      900

uucggaaaag ggucccuagu aaccugcgca aaauucgcau gcagcaagaa aaugacaggc      960

aaguccaucc agcccgaaaa ccucgaauau cgaauaaugc ucuccgucca cggcucacaa     1020

cacagcggaa ugaucgucaa cgacaccgga cacgaaaccg acgaaaaccg agcaaaaguc     1080

gagauaaccc caaacucacc ucgcgcugag gcaacacucg gaggcuucgg cucccucgga     1140

cucgacugcg aaccacgaac cgggcuagac uucagcgauc uauauuaccu caccaugaac     1200

aacaaacacu ggcuagucca caaagaaugg uuccacgaca uaccacuccc cuggcacgcc     1260

ggcgcagaca ccggcacgcc ccacuggaac aacaaagaag cccuggucga guucaaagac     1320

gcccacgcca aacgucaaac cgucguggua cucggguccc aagaaggagc aguccacaca     1380

gcacuugcgg gcgcccugga agccgaaaug gacggcgcca aaggacgucu cuccagcgga     1440

caccuaaagu gccgccugaa aauggacaaa cuacgacuaa agggagucuc auacagcuua     1500

ugcaccgccg ccuucacauu uaccaaaaua cccgcagaaa cgcuacacgg caccguaacc     1560

gucgaaguac aauacgcggg aacagacgga cccugcaaag uaccugcuca aauggccgua     1620

gacaugcaaa cacucacacc agucggaaga cuaaucacag caaaccccgu aaucaccgaa     1680

agcaccgaaa auuccaaaau gaugcucgaa cuagacccac cguuuggaga cucuuacauc     1740

guaauaggag ugggcgaaaa gaaaaucaca caucacuggc accgaucggg aucgaccaua     1800

gguaaagccu ucgaagcaac agugcgaggc gccaaacgaa uggcuguacu uggagacacc     1860

gcaugggauu uuggcuccgu uggaggagcc cuuaacagcc ucggcaaagg cauacaccag     1920

auuuucggcg cagccuucaa aucccucuuc ggcggcaugu ccugguucuc ccaaauccuc     1980

aucggaaccc uacucaugug gcuaggccuc aacacgaaaa acggaucaau aucccucaug     2040

ugccuagcac ugggcggugu acuaauauuc cucuccaccg ccgucucagc g              2091


<210>  2830
<211>  2088
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2830
aaagagaaaa agaggagagg cgcggacacg uccgucggua uaguaggccu acuucuaaca       60

accgccaugg ccgcagaagu aacccgucga ggaagcgccu acuacaugua ccucgaccga      120

aacgacgccg gcgaagcgau cagcuucccc accacccucg gcaugaacaa auguuacauc      180

cagauaaugg accucggcca caugugcgac gccacgaugu cauacgaaug ccccaugcuu      240

gacgaaggcg uugaaccaga cgacgucgac ugcuggugca acacuacaag caccugggug      300

gucuacggca cuugccacca caaaaaggga gaagcacgcc gcagccgucg agccguaacc      360

cuucccucac acagcacccg aaagcuccaa acccgcuccc aaaccuggcu agaaucucga      420

gaauacacca aacaccucau uagagucgaa aacuggauau uccgcaaucc aggcuucgcc      480

cuagcagcag ccgcaaucgc cuggcuucuc ggauccucaa caucacagaa agucauauac      540

cucguuauga uacuccuaau cgccccagcc uacagcauca gaugcaucgg cgucagcaac      600

cgagacuucg uagaaggcau gagcggagga acauggguag acaucguccu cgaacacgga      660

ggaugcguca ccguaauggc ccaagacaaa cccacaguug acauugagcu agucacaacc      720

accgugagca acauggccga agugcgcagc uacugcuacg aagccuccau aucagacaug      780

gcaucagaca gccgcugccc uacacaggga gaagcauacc uggacaaaca guccgacaca      840

caauacgucu gcaaacgaac ccucguagac cgaggcuggg gaaacggaug cggacuauuc      900

ggcaaaggcu cccucgucac augcgcaaag uucgccugca gcaagaaaau gaccggcaaa      960

ucuauccagc ccgagaaccu cgaauacaga auaaugcucu ccguacacgg aucccaacac     1020

agcggcauga ucgucaacga cacaggacac gaaacugacg aaaaucgcgc gaaagucgaa     1080

aucacaccca acuccccucg agcggaagcu acccugggcg gcuucggcag ccuaggccuc     1140

gauugcgaac cacgaacagg cuuagacuuc uccgaccucu acuaccuaac gaugaacaau     1200

aaacacuggc uaguccacaa agaaugguuc cacgacauac cguuaccgug gcacgccggg     1260

gccgauaccg gaacaccaca uuggaacaac aaagaagcac ugguagaguu caaagacgcc     1320

cacgcaaaac gccaaaccgu cgucguacuc gggucccaag aaggcgcagu ccacacagcc     1380

cucgcaggag cccucgaagc cgaaauggac ggcgcaaaag gccgccucag caguggucac     1440

cuaaaaugca gacuaaaaau ggacaaacuc cgacuaaaag gcgucucgua cucccugugu     1500

accgcagcuu ucacuuucac caagaucccc gcagaaaccc uccacggcac cgucaccgua     1560

gaagugcaau acgcagggac cgauggcccc uguaaaguac cggcccaaau ggcagucgac     1620

augcagaccc ugacacccgu gggccggcuc aucacagcaa accccguaau aacagaaucc     1680

accgaaaacu ccaagaugau gcuagagcua gaucccccau ucggcgacag cuacauaguc     1740

aucggaguag gagaaaagaa aaucacacac cacuggcacc gguccggcuc caccauaggc     1800

aaagcauuug aagcuacagu ccgcggcgcc aaacgcaugg caguacuugg cgacaccgcc     1860

ugggacuucg gcuccguagg aggggcgcuc aacagccuag ggaaaggaau ccaccaaauc     1920

uuuggagccg cauucaaauc ccuauucggc ggcauguccu gguucucuca aauccugauc     1980

ggcacccuac ucauguggcu aggccucaac accaaaaacg gcuccaucuc ccuaaugugc     2040

cuggcacucg gaggaguacu caucuuccua uccacagccg ucuccgca                  2088


<210>  2831
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2831
gagaagaaaa gaagaggagc agacaccagc guaggcaucg uaggccuccu acucacaacc       60

gcaauggccg ccgaaguaac ccgccggggc uccgcauacu acauguaccu agaccgcaau      120

gaugccggcg aagcaauauc guuccccacc acccucggca ugaacaaaug cuacauacaa      180

aucauggacc uaggacacau gugcgacgcc accauguccu acgaaugccc caugcuagac      240

gaagggguug aacccgauga cgucgacugc uggugcaaua caacgagcac cugggugguu      300

uacggaacgu gccaccacaa gaaaggggag gcccgccgcu cccgcagagc cguaacccuc      360

cccucacaca gcaccagaaa acuacagaca cgaagccaga cauggcucga aucacgagaa      420

uacaccaagc accucauccg cguagaaaac uggauauucc gcaaccccgg cuucgcccua      480

gccgcagccg ccaucgccug gcuccuuggc uccucaacaa gccaaaaagu cauauaccuc      540

gucaugauuc uacucaucgc gcccgccuac uccauccguu gcaucggagu cuccaacaga      600

gacuucgucg aaggcauguc aggaggcacc uggguugaca ucguccuuga acacgggggu      660

ugcguaacgg ugauggccca ggacaaaccc acaguagaca uagaacuugu gaccacaacu      720

guauccaaca uggcggaagu ccgcucauac ugcuacgaag ccagcaucuc ggacauggcc      780

uccgacuccc gaugcccaac acaaggcgaa gcauaccucg acaaacaauc cgacacccaa      840

uacgucugca aacggacacu cgucgaccgc ggauggggaa acggcugugg ucuauucggc      900

aaaggauccc uagugaccug cgcaaaauuu gcuugcucaa agaaaaugac gggaaaauca      960

auccagcccg aaaaccucga auacagaauc augcuuuccg uccacggcag ccaacacagc     1020

ggaaugauag ucaacgacac cggccaugaa accgacgaaa accgggccaa aguagaaauc     1080

accccaaacu caccccgcgc agaagccacc cucggagggu ucggaagccu cggacucgac     1140

ugcgaaccac gcacuggacu agacuucuca gaccuguacu aucucacgau gaacaauaaa     1200

cauuggcuag uacacaaaga gugguuccac gauauaccac uaccauggca cgccggagca     1260

gacacaggca caccccacug gaacaacaaa gaagcccucg uagaguucaa agacgcacac     1320

gccaaacguc aaaccgucgu aguacucggu ucacaagagg gcgcuguaca caccgcacua     1380

gccggcgccc ucgaagccga aauggaugga gccaaagggc gccuauccuc aggccaccua     1440

aaaugccgac ucaaaaugga caaacuucgc cucaaagggg uaucauacuc ccuaugcaca     1500

gccgccuuca ccuucacaaa gauacccgcc gaaacauuac acggcaccgu cacagucgag     1560

guccaauacg ccggcaccga cggacccugc aaagucccgg cacagauggc aguagacaug     1620

caaacccuca cacccgucgg ccgccuaaua acagcaaacc ccgucaucac cgaaucaacc     1680

gaaaacagca aaaugaugcu ggaacucgac ccacccuucg gcgacuccua caucguaauc     1740

ggcguaggcg aaaagaaaau aacccaucac uggcacaggu ccgguagcac caucggaaaa     1800

gcauucgaag caaccguccg aggcgccaaa cggauggcug uacuaggaga cacugccugg     1860

gacuucggcu ccguuggcgg cgcccuaaac ucgcuaggca aaggaaucca ccaaaucuuc     1920

ggcgccgccu uuaaaagccu auucggagga augagcuggu ucucacaaau ccucaucgga     1980

acacuacuaa uguggcuggg acugaacacc aaaaacggaa gcauaucacu uaugugccuc     2040

gcacuaggug gcguucuuau cuuccucagc acagcgguau cugca                     2085


<210>  2832
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2832
aagaaacggc gaggcgccga cacaagcguc ggcaucguag gccuacuccu aaccaccgcu       60

auggcagccg aaguaacacg ccgaggaagc gcauacuaua uguaccuaga ucgcaacgac      120

gcaggcgagg ccaucagcuu ccccaccaca uuaggcauga auaaaugcua cauccaaaua      180

auggaucuag gccacaugug cgaugccaca augaguuacg aaugcccaau gcucgacgag      240

ggagucgaac ccgacgacgu agacugcugg ugcaacacca cuucaacaug ggucguauac      300

ggaaccugcc accacaagaa aggcgaagca aggagaucaa ggagagcugu cacgcucccc      360

ucacacagca cccgcaaacu acaaacacgg ucccaaaccu ggcuggaaag ccgagaguau      420

acaaaacacc uaauccgcgu cgaaaauugg auauucagaa accccggcuu cgcgcucgca      480

gcagccgcaa uagcauggcu acucggcuca agcacuucac aaaaagucau cuaucuaguc      540

augauccuuc ucaucgcacc agcauacagu auacgcugca uagggguaag caaccgggac      600

uucguugaag gaauguccgg gggcacgugg guagacauag uacuagaaca cggaggaugc      660

guaacgguaa uggcccaaga caaacccaca guggacaucg agcuaguaac gacaacaguc      720

agcaacaugg ccgagguacg gucauacugc uacgaggcaa gcauaucaga cauggcaucc      780

gacucaaggu gccccacaca gggcgaggcc uaccucgaca agcaaagcga cacacaauac      840

gucugcaaac ggacccucgu agauagaggc uggggcaacg gaugcggccu cuucggaaaa      900

gggagccuag uaacaugcgc aaaauucgcc ugcagcaaga aaaugaccgg aaaaagcaua      960

caaccagaaa accuugaaua ccgcauaaug cuaagcguac acggcagcca acacagcggu     1020

augaucguaa acgacacagg acacgaaacc gacgaaaaua gagcaaaagu agaaauaaca     1080

ccgaacuccc cucgggcaga agccacacuc gggggauucg gaagccuagg ccuagacugc     1140

gagcccagaa ccggacugga cuucagcgac cucuacuacc uaacaaugaa caacaagcac     1200

uggcuaguac acaaagaaug guuccacgac auaccucucc cauggcacgc aggggcagac     1260

acaggcacac cccacuggaa caauaaagaa gcccuagugg aguucaaaga cgcccacgca     1320

aaaagacaaa ccguaguagu ccucggaagc caagaaggcg ccguccacac cgcgcuagcc     1380

ggggcgcugg aagcggaaau ggacggcgcc aaaggacgac uaagcagcgg ccaccuaaaa     1440

ugccgacuaa aaauggacaa acuaaggcuc aaaggcguau ccuacucacu cugcacagcc     1500

gccuucaccu ucacaaaaau accagcagag acgcuccacg gcacagugac gguggaaguc     1560

caauaugccg gcacagacgg accaugcaag gugccagcac aaauggcggu cgacaugcaa     1620

acacuaacac ccguaggccg ccuaauaacc gccaaccccg uaaucaccga aagcacggaa     1680

aauucgaaaa ugaugcuaga gcucgaccca ccguucggag acucauacau cgucauagga     1740

gugggcgaga aaaagauaac acaccacugg caccgaagcg gcuccacaau cggaaaagca     1800

uucgaagcca caguaagagg ggccaagaga auggccguac ucggagauac agcaugggau     1860

uucgggucag uaggcggagc ccuaaacagc cuaggcaaag gcauccacca gaucuucgga     1920

gcagcauuca aaagucucuu uggaggaaug uccugguucu cccaaauacu gaucggcaca     1980

cuacugaugu ggcugggacu aaacaccaaa aacgggagca ucucacucau gugucuggca     2040

cugggcggag uacucaucuu ccuaagcaca gccguaucag ca                        2082


<210>  2833
<211>  2079
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2833
aaaagacgcg gcgcggacac cuccgucggc auagucgguc uucugcucac aacagcaaug       60

gccgcagagg ucacacgaag aggcagcgcc uacuacaugu accucgaccg aaacgacgca      120

ggcgaagcaa ucucauuccc cacaacccua ggcaugaaca aaugcuacau ccagauaaug      180

gaccuaggcc acaugugcga cgccacaaug uccuacgaau gcccuauguu agacgaagga      240

guugaaccag acgacguaga cugcuggugc aacacaacau caacgugggu gguguauggc      300

acaugccauc acaagaaagg agaagccaga cgcagcagac gagcagucac ccuacccagc      360

cacucaacac gaaaacucca aacacgguca cagacauggc ucgaaagccg cgaauacacg      420

aaacaccuaa uccgggucga gaauuggauc uuccgcaauc caggauucgc ccucgcagcc      480

gcagccaucg ccuggcuacu aggaagcucc accucacaaa aagucaucua ccucgucaug      540

auacuccuaa uagcaccagc cuacuccauc agaugcauag gcgucagcaa ccgcgacuuc      600

guagaaggaa ugaguggcgg caccuggguc gacaucgucc uagaacacgg cggaugcgua      660

accgugaugg cgcaggacaa acccaccgug gacauagagc uagucaccac caccgucucg      720

aacauggcag aaguacgauc cuacugcuac gaagccagca uaagcgacau ggcaagcgac      780

agucgcugcc ccacccaagg agaagccuac cuagauaaac aaagcgacac ccaauacguc      840

uguaaacgaa cccuagucga ccgcggaugg ggcaacggcu guggacucuu cgggaaaggc      900

ucacugguga caugcgccaa auucgcgugc ucuaagaaaa ugacagggaa aucuauccaa      960

cccgaaaacc uagaauaccg caucaugcua agcguacacg gcucacaaca cuccggcaug     1020

auaguaaacg acacaggaca cgaaaccgac gagaacagag ccaaaguaga aaucacgccc     1080

aacuccccca gggccgaagc aacccucggc ggcuucggau cccuugggcu agacugcgaa     1140

cccagaaccg gacucgacuu cagcgaccuc uacuaccuaa caaugaacaa caaacacugg     1200

uuagugcaca aggaaugguu ccacgacauc ccacuaccau ggcacgcggg agcagacacu     1260

ggcacacccc acuggaacaa caaagaagcu cucgucgagu ucaaggacgc ccacgccaaa     1320

aggcaaacag ucgucguccu agggagccag gaaggagccg ugcacaccgc acucgcuggc     1380

gcccuggaag ccgaaaugga cggagccaaa ggcagacuau caagcggaca cuuaaaaugc     1440

agacuaaaaa uggauaaacu ccgucuaaaa ggcguaagcu acagccuaug caccgccgcg     1500

uucacauuca ccaaaauacc agcagaaacu cuccacggca ccgugacagu agaaguccag     1560

uacgccggca cggauggacc auguaaagua ccggcccaaa uggcaguaga caugcaaaca     1620

cucacacccg uaggacgccu aaucaccgca aaccccguga uaaccgaauc aaccgaaaac     1680

agcaaaauga ugcucgaacu cgacccacca uuuggagaua gcuacaucgu gaucggggua     1740

ggagagaaaa agaucacgca ccauuggcac cgcucaggcu caaccauagg aaaggccuuc     1800

gaagcaaccg uacgaggagc aaaacgcaug gcaguacuag gagacacugc cugggacuuc     1860

ggcucaguag gcggagcacu caacucccua ggcaaaggaa uacaccaaau cuucggcgcc     1920

gccuucaaaa gccucuucgg cgguaugucc ugguucagcc aaauccugau cggcacacuu     1980

cuaauguggu uaggacucaa caccaaaaac ggcuccaucu cccuaaugug ccuagcacuc     2040

ggcggcguac ucaucuuccu aagcacagca guguccgca                            2079


<210>  2834
<211>  2076
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2834
agacgaggcg ccgacacauc aguuggaaua guaggacuac uacuaaccac agccauggcc       60

gcagaaguaa caagacgcgg auccgccuau uacauguacc uagaccgaaa ugacgcaggc      120

gaggcgauca gcuucccaac aacgcuggga augaacaaau gcuacauaca aauaauggac      180

cuuggccaca ugugugacgc aacaaugagc uacgaaugcc ccaugcucga cgagggcguc      240

gaaccagacg acguagacug cuggugcaau acaacaagca ccuggguagu cuacggaaca      300

ugccaccaca aaaagggaga agcacgacgc agccgccgag cgguaacccu ccccagccau      360

ucgacccgga aacuacagac ccgaagccaa accuggcuag aaucacgcga auacaccaaa      420

cacuuaauca gaguggagaa cuggaucuuc cgcaaccccg gauucgcacu agcagcagcc      480

gcaauagcau ggcuccuagg cagcagcaca agccaaaagg ucauauaccu cgugaugauc      540

uuacucauag ccccagccua cagcauacga ugcauaggcg uguccaacag agacuucguc      600

gaaggcauga gcggaggcac auggguagac auaguacucg aacacggcgg augcguaacg      660

gugauggccc aagacaaacc aacaguagau aucgaacugg ucaccaccac agucagcaau      720

auggcagaag uaagauccua cugcuacgaa gcauccauaa gcgacauggc aagcgauagc      780

agaugcccca cacagggcga agccuaccuc gacaaacaau cagauacaca auacgucugc      840

aagcgcaccc uggucgacag aggcugggga aauggaugcg gccuauuugg aaaagggucc      900

cuggucacgu gcgcaaaguu cgcgugcagc aagaaaauga ccggcaaaag cauccaaccc      960

gagaaccucg aauacagaau aaugcucagc gugcacggga gccaacacag cggcaugaua     1020

guaaacgaca cuggacacga aaccgaugag aaccgagcca aagucgaaau aaccccuaac     1080

ucaccacgag cagaagcgac acuagggggc uucggcaguc ucgggcuaga cugcgagccc     1140

agaaccggcc ucgacuuuuc cgaccucuac uaccuaacca ugaacaacaa acacuggcuc     1200

gugcacaaag aaugguucca cgacauccca cucccauggc acgcaggagc agacacagga     1260

acaccccacu ggaacaacaa agaggcacuc gucgaguuca aggacgccca cgcaaaaaga     1320

caaacaguag uaguucucgg aagccaagaa ggagcaguac acaccgcccu ugcaggggcc     1380

cucgaagccg agauggacgg agcgaaaggc cggcuaucgu caggccaccu caaauguaga     1440

cuaaagaugg acaaacucag acuaaaagga gugucauaca gccugugcac agccgcguuc     1500

acauucacaa aaauaccggc ggaaacacuc cacgggacgg ucaccguaga ggugcaauac     1560

gccggcaccg acggccccug caaagucccg gcacagaugg ccgucgacau gcaaacccuc     1620

acaccaguag gccgacuaau cacagccaac ccgguaauaa cggaauccac agaaaacuca     1680

aaaaugaugc uagaacucga cccaccauuu ggcgacagcu acauagugau agggguaggc     1740

gaaaagaaaa ucacacacca cuggcaccga agcggcagca ccauagggaa agcauucgaa     1800

gcaaccguac ggggagcaaa acgcauggca guacucgggg acacagccug ggacuucggc     1860

agcguaggcg gugcacuaaa cucacuaggc aagggcauac accaaauauu cggggcggcc     1920

uucaaaucuc uauucggggg caugagcugg uucucacaaa uccuaaucgg gacccuccuc     1980

auguggcuag gacucaauac aaagaacggc agcauaucgc ucaugugccu ggcauuagga     2040

ggaguccuaa ucuuccucag cacagcagua agcgca                               2076


<210>  2835
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2835
cgcggagcag acaccagcgu cggcaucgug ggccuccuuc ugacaacagc cauggccgcg       60

gagguaacac gaagaggcag cgcguacuac auguaccuag acagaaauga cgcaggagaa      120

gccaucucgu ucccaacaac ccuaggaaug aacaaaugcu acauccaaau cauggaccua      180

ggccacaugu gcgacgccac caugagcuac gaaugcccaa ugcucgacga aggaguagag      240

cccgacgacg uagacugcug gugcaacacc accagcacau ggguaguaua cggaaccugc      300

caccacaaaa agggggaagc ucgccgaagc cgccgcgcug ucacacuccc cagccacagc      360

accagaaaac uccaaacccg cucacaaacc uggcuagaaa gccgagaaua caccaaacac      420

uuaauccgag uggaaaacug gauauucagg aaucccggcu ucgcccuugc cgccgccgca      480

auagccuggc uccucggcag cucaaccuca caaaaaguca uauaccuagu caugauacuc      540

cuaauagccc ccgccuacuc cauaagaugc auaggcguaa gcaacagaga cuucguagaa      600

gggaugucug gcggcaccug gguagacauu guacucgaac acggcggcug ugugacagug      660

auggcccaag acaaaccaac cguagacauc gagcuaguca caaccaccgu cuccaacaug      720

gccgaaguca gauccuacug cuacgaagcg agcauauccg acauggcguc cgacagccga      780

ugccccacac agggcgaagc cuaccucgac aaacaaagcg acacccagua cguauguaaa      840

cgcaccuuag uggacagagg auggggcaac ggcugcggac ucuucggcaa gggaucccuc      900

gucacaugcg ccaaauucgc augcucgaag aaaaugaccg gcaaaagcau ccaaccagaa      960

aaccucgaau accgaauaau gcucucggua cacggcagcc aacacagcgg aaugaucguu     1020

aacgacaccg ggcacgagac agacgaaaac cgugccaaag ucgaaauaac ccccaacagc     1080

ccaagagcag aggccacccu cggcggauuc gguagccuag gacuagacug cgagcccaga     1140

accggccugg acuucucaga ccucuacuac cuaacuauga acaacaaaca cuggcuaguc     1200

cacaaagaau gguuccacga cauaccccua cccuggcacg ccggagcaga cacaggcacc     1260

ccacacugga acaacaaaga agcccuagua gaguucaagg acgcacacgc aaaacgccaa     1320

acaguuguag uccuuggcag ccaagaagga gccguccaca ccgcccucgc aggagcccua     1380

gaagcagaaa uggauggcgc gaaaggccgc cuaucauccg gccaccuaaa augcagacuc     1440

aagauggaca aacuaagacu caagggaguc uccuacuccc uauguaccgc agccuucacg     1500

uucaccaaaa ucccagcaga aacacuccac ggcaccguaa ccgucgaagu acaauaugcc     1560

gguacagacg gacccugcaa gguaccagcc caaauggccg uggacaugca aacccuaaca     1620

cccguaggcc gccugaucac agcgaacccc guuaucaccg aauccacaga aaacucaaag     1680

augaugcucg aacuagaccc acccuucgga gacuccuaca uagucaucgg cgucggggaa     1740

aagaaaauaa cacaccacug gcaccgcucc ggcagcacca uagguaaagc auucgaggcc     1800

accguacgag gagccaaacg aauggccgua cuaggcgaca ccgccuggga cuuuggaucc     1860

gugggcggcg cacuaaacuc acuaggcaaa ggaauacacc aaaucuucgg ggcagccuuc     1920

aaaagccuau ucggcggaau gucuugguuc ucacaaaucc ucauaggaac acuccugaug     1980

uggcucggac ugaacaccaa aaacggaucc auaagucuaa ugugccuagc acugggcgga     2040

guccuaaucu uccugagcac cgcaguaucc gcg                                  2073


<210>  2836
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2836
ggcgccgaca caucggucgg aauagucgga cuacuccuca caacagccau ggcagcggaa       60

gucacgcgaa gaggcuccgc cuacuacaug uaccucgacc gcaacgacgc aggggaagcg      120

aucagcuucc ccaccacccu cgggaugaac aaaugcuaca uacaaauaau ggaucuagga      180

cacaugugcg acgcgacgau gagcuacgaa ugccccaugc uagacgaagg aguagaacca      240

gacgacgucg acuguuggug caacacaaca uccacuuggg ugguauaugg aaccugccau      300

cacaagaaag gagaagccag gaggucccgc agagccguca cacuccccag ccacuccacu      360

cgcaagcuac aaacaagauc acaaaccugg cuagaaagca gggaauauac aaaacaccua      420

auaagagugg aaaacuggau cuucagaaac cccggcuucg cccuagccgc agcggccaua      480

gccuggcuac ucggcagcuc cacaagccag aaagucaucu accucguaau gauccuucug      540

aucgccccag cguacuccau acgguguaua ggaguaucca accgcgacuu cgucgaaggc      600

augucaggcg gaacaugggu cgacauaguc cucgaacacg guggaugcgu cacgguaaug      660

gcacaggaca aacccacggu cgauaucgag cuaguaacaa ccacggucuc caacauggcc      720

gaaguccgcu cauacugcua cgaagccucc auauccgaca uggcguccga cucccgcugc      780

ccaacccaag gcgaagccua ccucgacaaa caauccgaca cccaauacgu augcaaacga      840

accuuagucg accgcggaug ggggaacgga uguggccuau ucggcaaggg cucgcucguu      900

acaugcgcca aauuugccug cucgaagaaa augacgggaa aaagcaucca acccgaaaac      960

cuagaauaca gaauaaugcu aucgguccau ggaucacaac acucagggau gauaguaaac     1020

gacacgggac acgagaccga cgaaaaccgc gcgaaagucg aaauuacccc aaacaguccu     1080

cgcgcagaag caacccuagg cggguucgga agccucggcc ucgacugcga accacggaca     1140

ggcuuagacu uuagcgaccu guacuaccuc accaugaaca acaaacacug guuaguccac     1200

aaagaauggu uccacgacau accccuacca uggcacgccg gagccgauac agguacaccc     1260

cacuggaaca acaaggaagc gcuaguggag uucaaagacg cccacgcaaa acgccaaacc     1320

gucgucgucu uaggcagcca agaaggagcc guccacaccg cgcucgccgg agcccuagaa     1380

gcagagaugg acggagcaaa aggacgccua aguucagggc accuaaagug ccgacugaaa     1440

auggacaagc ugcgucucaa aggcgugucc uacucacuau gcacggcggc auuuacguuc     1500

accaaaaucc ccgcagaaac ccuacacgga accgucaccg uagaagucca auaugccgga     1560

acggacggac ccugcaaggu cccagcccaa auggccgucg acaugcagac ccugaccccc     1620

guaggccggc uaauaaccgc aaacccggua auaacagaau caacagagaa cucaaaaaug     1680

augcucgaac uggacccacc cuucggcgac uccuacauag ucaucggagu aggagaaaag     1740

aaaauaaccc accacuggca ccgcagcgga agcaccauag gcaaagcguu cgaggcgacc     1800

guccgcggcg ccaaaaggau ggcaguccua ggagacaccg caugggacuu cggaagcgua     1860

ggaggcgccc ucaacucacu aggaaaagga auccaccaaa ucuucggagc ggcguucaaa     1920

agccucuucg gcggaaugag cugguucuca caaauccuca ucggaacccu gcuaaugugg     1980

cucggacuaa acaccaaaaa cgggagcauc ucccuaaugu gucucgcacu agguggcguc     2040

cucaucuucc ucagcacagc aguaucagcg                                      2070


<210>  2837
<211>  2067
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2837
gccgacacaa gcguaggaau aguaggacug cuccugacca cagcaauggc agcagaagua       60

acaagacgag gcuccgcgua uuacauguac uuagaccgaa acgacgccgg agaagccaua      120

ucuuucccca ccacccuagg gaugaacaag ugcuacauuc aaauaaugga cuugggccac      180

augugcgaug cgaccaugag cuacgagugc cccaugcuag acgagggcgu cgaaccagac      240

gacguagacu gcuggugcaa cacgaccuca acauggguag uauacgggac augccaccac      300

aagaaaggag aggcacgaag aagccgacga gccguaacgc uacccuccca cucgacacgc      360

aaacuccaaa caagauccca aacauggcug gaaagcaggg aauacacaaa acaccucauu      420

agaguagaaa acuggauauu ucgaaaccca ggcuucgccc uagcagcagc agcgaucgcc      480

uggcuccucg gaagcagcac cucgcaaaaa guaauuuacc ucguaaugau acuacuaauc      540

gcgccagcau acagcaucag guguaucgga guaagcaaca gagacuucgu agaaggcaug      600

ucaggaggaa ccuggguaga caucguacua gaacacggag gaugcguaac cgugauggcu      660

caagacaaac caaccguaga caucgagcua guaaccacua cagucuccaa uauggcugaa      720

gucagauccu acugcuacga agccucaauu agcgacaugg cgagcgacag ccgaugcccg      780

acccaaggag aagccuaccu cgacaaacaa ucggacaccc aauacguaug caaaaggaca      840

cuggucgacc gaggcugggg gaacggcugc ggccuguucg ggaaaggcag ccucguaaca      900

ugcgcgaaau ucgccugcuc caagaaaaug acuggaaaau caauccaacc agaaaaccug      960

gaauacagaa ucaugcucuc cguacacggg agccaacaca gcggaaugau cguaaacgac     1020

accggccacg aaaccgacga aaaccgcgcg aaagucgaaa uaacgcccaa cucacccaga     1080

gccgaggcca cacuaggagg uuucggauca cuagggcucg acugcgaacc caggaccgga     1140

cuagacuuca gcgaccucua cuaccucaca augaacaaca aacacuggcu agugcacaaa     1200

gaaugguuuc acgacauacc ccuacccugg cacgcaggcg cagacaccgg cacgccccac     1260

uggaacaaca aagaagcacu cgucgaguuc aaagacgccc acgccaaacg ccagaccgua     1320

gucguccuag gaagccaaga aggagcaguc cacaccgccc uagcaggggc ucuagaagcc     1380

gaaauggacg gcgcaaaggg acgacucagc agcggccacc uaaaaugccg ccugaaaaug     1440

gacaaacuca gacucaaagg aguaucauac ucacuaugca cagcagcauu cacauucacc     1500

aaaauccccg cagaaacccu ccauggaaca gucacagucg aaguucaaua ugccggcaca     1560

gacggaccau gcaaaguacc cgcacaaaug gccguagaca ugcagacgcu aacccccguc     1620

ggaaggcuca uaacagcuaa ccccgucaua accgaaagca cagaaaacuc caaaaugaug     1680

cucgaacucg aucccccguu cggcgacucg uacauuguca uaggcgucgg agaaaagaaa     1740

aucacacacc acuggcaccg cucaggcagc acaaucggca aagccuucga agcgacagua     1800

cggggagcca aacgaauggc cguccucgga gacacagccu gggacuucgg aagcgucgga     1860

ggagcccuua acagucuggg aaaaggaauc caccaaaucu ucggggcagc uuucaaaagc     1920

cucuucggcg gcaugagcug guucagccaa auccuaaucg gaaccuuacu aauguggcuc     1980

gggcugaaca cgaaaaacgg guccauaagc cucaugugcc ucgcccuggg cggaguccuc     2040

auauuccuaa gcacagccgu cuccgcc                                         2067


<210>  2838
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2838
gacacaagcg ucggaauagu gggucuccua cucacgaccg caauggccgc agagguaacc       60

cgaaggggcu cugcauacua cauguaccug gaccgcaacg acgcaggcga agccauauca      120

uuccccacga cacucggaau gaacaaaugc uauauacaga ucauggaccu cggacauaug      180

ugcgacgcca ccauguccua cgaaugcccc augcuagacg aaggcgugga accagacgac      240

gucgacugcu gguguaacac cacgagcacc uggguagucu acggcacaug ccaccacaag      300

aaaggggaag ccagaagauc acgccgagcg guaacgcucc cgucacacuc cacaagaaaa      360

cuacagacac gaagccaaac cuggcucgaa agccgcgaau acaccaagca ccuaauaaga      420

guagagaacu ggauuuuccg aaacccuggg uucgcgcuag cagcugcagc aauagccugg      480

cuccuaggga gcucaacguc acagaaaguc aucuaccucg ucaugauacu acugaucgca      540

cccgccuacu ccauccgaug cauaggcguc ucaaaccgcg acuucguaga aggcaugucc      600

ggagggaccu gggucgauau cguucucgaa cacggaggau gcguaacagu cauggcccag      660

gauaaaccca caguagacau agaacucgua acaacgacag ucucaaacau ggccgaagua      720

agguccuacu gcuacgaagc aucaaucucg gacauggccu ccgacucacg augccccacc      780

caaggcgaag ccuaccucga caaacaauca gacacccaau augucuguaa acggacccuc      840

guagaccgcg gcuggggcaa cggaugcggc cuauucggca aaggcucccu agugaccugc      900

gccaaauucg caugcucaaa gaaaaugaca ggcaaaagca uacaacccga aaacuuagaa      960

uaccguauca ugcuuuccgu acacggcagc caacacuccg gcaugaucgu caaugacacc     1020

ggccacgaaa cugacgaaaa uagagcaaaa guagaaauca caccaaacag cccacgagcg     1080

gaagcgacgc ucggcggcuu uggcucacuc ggccucgacu gugagccaag aacaggacua     1140

gacuucuccg aucuauacua ccucacgaug aacaacaaac auuggcuagu acacaaagaa     1200

ugguuucaug auaucccccu accuuggcac gccggagccg acacaggaac accucacugg     1260

aacaauaaag aggcacucgu agaguucaaa gacgcacacg ccaaaagaca aaccguaguc     1320

guccuuggcu cacaagaagg cgccguacac accgcccucg ccggagcacu cgaagccgag     1380

auggacggcg ccaaaggccg acuaucuagc ggccaccuca agugcaggcu caagauggau     1440

aaacuacgac ucaagggagu aucauacucc cuaugcaccg cagcauucac guucacaaaa     1500

auacccgcag aaacccuaca cggcacaguc acaguggagg uacaguacgc cggcacagac     1560

ggaccaugua aagucccagc acagauggcc guugacaugc agacccuaac ccccgucggg     1620

agacucauca cagcaaaccc aguaaucaca gaaagcacag aaaacucaaa gaugaugcuc     1680

gaacuugacc cacccuucgg cgacuccuac aucgucauag gaguaggaga gaagaaaaua     1740

acccaccacu ggcaccgcuc cggaaguaca auaggcaaag cauucgaagc caccguccga     1800

ggcgcaaaac gcauggcggu ccuaggcgac accgcguggg acuucggaag cgucggcggc     1860

gcacucaacu cucugggaaa aggcauacac cagauuuucg gcgcagccuu caaaucccua     1920

uucggcggca ugagcugguu cagccaaaua cucaucggca cucuccuaau guggcuaggc     1980

cucaacacga aaaacggcag cauaagccua augugccucg cccuaggagg aguccuaaua     2040

uuccuaucaa cagcagugag cgcu                                            2064


<210>  2839
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) CDS opt

<400>  2839
accagcgucg ggaucgucgg ccuccuacua accacagcaa uggcagcaga aguaaccagg       60

cgcgguuccg ccuacuauau guaccucgac agaaacgacg cgggcgaagc caucuccuuu      120

ccaaccacac ucggcaugaa caaaugcuac auccaaauaa uggaccucgg acacaugugc      180

gacgccacga uguccuacga augucccaug cuagacgaag gugucgaacc agacgacgua      240

gacugcuggu gcaacaccac cagcaccugg gugguauacg gcacaugcca ccacaagaaa      300

ggcgaagcca gacgaucgcg ccgagccguc acgcuccccu cccacucgac cagaaaacua      360

caaacccgcu cucagaccug gcucgaaucg cgagaauaua ccaaacaccu uauacgaguc      420

gaaaacugga uauuccgcaa cccaggauuu gcauuagccg ccgcagcaau cgccuggcua      480

cucgggagcu caacaucaca aaaaguaauu uaccucguca ugauccugcu cauugcccca      540

gcuuacucaa uacgauguau cgggguaagc aaccgagacu uugucgaagg caugagcgga      600

gggaccuggg ucgacaucgu ccucgaacac ggcggcugcg uaacagucau ggcccaggau      660

aaacccaccg ucgacauuga gcuagucaca accacagucu cgaacauggc cgaaguacgc      720

agcuacugcu acgaagcguc cauaagugac auggccagcg acucccgcug ccccacucaa      780

ggcgaagccu accucgauaa acaaagcgac acccaauacg uaugcaaaag aacccucguc      840

gaccgaggcu ggggcaacgg uugcggccua uucggaaaag gcucccuagu uaccugcgca      900

aaguucgcau gcucaaagaa aaugaccggc aaauccaucc aaccagaaaa ccuggaauac      960

cgcauaaugc ugagcgucca cggcucccaa cacuccggaa ugaucgucaa cgacacaggg     1020

cacgaaacgg acgaaaaccg cgcaaaaguu gaaauaaccc ccaauagccc ccgcgcagaa     1080

gcaacacucg gcggcuucgg aagccuaggg cucgacugcg aaccccgcac aggccucgac     1140

uucuccgacc uguacuaccu aaccaugaac aacaaacacu ggcucgucca caaagaaugg     1200

uuccacgaca ucccacuacc auggcacgcg ggugcagaca caggcacacc ccacuggaau     1260

aacaaagaag cccucguaga guucaaagau gcccacgcaa aaagacagac cgucguaguc     1320

cucggcaguc aagaaggcgc cguccacacg gcucucgccg gagcccuaga agccgaaaug     1380

gacggagcaa aaggaaggcu auccucaggc caccucaaau gcaggcucaa gauggacaaa     1440

cuaagacuca aaggcguaag cuacagccuc ugcacagccg cauucaccuu cacgaaaauu     1500

cccgcggaaa cgcuacacgg cacagugaca gucgaagucc aguacgcagg cacagacggg     1560

ccaugcaaag uacccgcaca aauggcgguc gacaugcaga cacucacccc cguaggaaga     1620

cuuaucacag caaaccccgu aaucacagaa uccacagaaa acagcaaaau gaugcuagaa     1680

cuagauccac ccuucggaga cuccuacauc guaauaggcg ucggagaaaa gaaaaucacu     1740

caccauuggc acagaagcgg cuccacaaua gggaaagccu ucgaggcgac aguacgcgga     1800

gcuaaacgca uggccguacu cggggacacc gccugggacu ucggcuccgu cggcggcgca     1860

cuaaacaguc ucggcaaagg aauacaccaa aucuucggcg ccgccuucaa aucccuauuc     1920

ggaggcauga gcugguucag ccaaauccug auaggcacau ugcucaugug guuaggccuc     1980

aacaccaaaa acggcagcau cagccucaug ugccucgcac ucgggggagu acuaauauuc     2040

cuauccaccg ccgucuccgc c                                               2061


<210>  2840
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2840
accuccguag gcaucguggg acuccuccuc acaaccgcaa uggccgcagc cguaaccaga       60

cgcggcuccg cauauuacau guaccucgac agaaacgacg ccggcgaagc gaucucguuu      120

cccaccaccc ucgggaugaa caaauguuac auccaaauca uggaccucgg ccacaugugc      180

gacgccacca ugagcuacga augccccaug cuagaugaag gaguagaacc agacgacgua      240

gacugcuggu gcaacaccac aucaacaugg guaguguacg gcacauguca ccacaagaaa      300

ggugaagcac ggcgcagcag acgcgcggua acgcuccccu cacacuccac acgcaaacuc      360

caaaccagaa gccaaaccug gcuagaaagc cgagaauaca ccaaacaccu cauacgaguc      420

gaaaacugga uauucagaaa ccccggauuc gcccucgcgg ccgcggcaau agcuuggcua      480

cucggauccu cgaccucaca aaaagucauc uaccugguca ugaucuuacu cauagcacca      540

gcauacucga uccgguguau cggcgugucu aaccgcgacu uugucgaggg cauguccggc      600

ggcaccuggg uggacaucgu ccuagaacac gggggcugcg ucaccguuau ggcccaagac      660

aaacccacgg uagacauaga gcucgucacc accacaguau ccaacauggc cgaaguccga      720

ucauacugcu acgaagccuc cauaagcgac auggccucag acucccgcug ccccacacaa      780

ggugaagcau accuagacaa acaaucagac acgcaauacg ugugcaaacg cacucugguc      840

gaccgaggcu ggggcaacgg cugcggccuc uucggcaagg gaagccuagu aaccugcgcc      900

aaauucgccu gcucaaagaa aaugaccggc aaaucaaucc aacccgaaaa ccucgaauac      960

cgaaucaugc uaucagucca cggaucacag cacucuggca ugauagucaa cgacacaggu     1020

cacgaaacag acgaaaaccg cgccaaaguu gaaaucaccc ccaacucacc ucgagcagaa     1080

gccacccucg gaggauucgg cucccuaggc cuagacugcg aaccacgcac aggacuagac     1140

uucagcgacc ucuauuaccu caccaugaac aacaaacauu ggcuaguuca caaagaaugg     1200

uuccacgaca uaccccuccc auggcaugcc ggugcagaca ccggaacacc ucacuggaac     1260

aacaaggaag cccucguaga guucaaggac gcacacgcaa aacgacaaac agucguaguc     1320

cucggaagcc aggaaggagc cguucacacc gcacuugcag gagcccugga agccgaaaug     1380

gacggugcca aaggccgccu uuccucaggg caccucaaau gccgccucaa aauggacaaa     1440

cuacgccuca aaggcgucuc uuacucccuc uguacugccg ccuuuaccuu caccaagaua     1500

ccagcagaaa cucugcacgg caccgucaca gucgaaguac aauacgcugg caccgacgga     1560

cccugcaaag uccccgccca aauggccguc gacaugcaga cccuaacccc cguaggaaga     1620

cuaaucacag ccaauccagu caucaccgaa uccacagaaa acucaaaaau gaugcucgaa     1680

cucgauccac ccuucggaga cagcuacauu gucaucggcg ucggugaaaa gaaaauaaca     1740

caccacuggc aucgaucagg cucaacgauc ggaaaagcgu ucgaagcuac aguacgaggc     1800

gccaaacgga uggccguccu cggcgacacc gcuugggacu ucggcuccgu cggaggugca     1860

cucaacucac ucggcaaagg uauccaccaa aucuucggcg cagcguucaa gagccucuuc     1920

ggcggcaugu caugguuuuc acaaauacuc auaggaacac uacucaugug gcucgggcuc     1980

aacacaaaga acggcagcau aagccucaug ugccucgcac uuggcggcgu ccuaaucuuc     2040

cuaucgacag caguguccgc c                                               2061


<210>  2841
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2841
cgcggcgcgg acacaucagu cggcaucguc gggcuacuac ucacgacggc cauggcagcc       60

gcugucacaa gacgaggaag ugccuauuac auguaccucg accgcaacga cgccggggaa      120

gccaucuccu uccccacaac acucggcaug aacaaaugcu acauccaaau cauggaccuc      180

ggccauaugu gcgacgccac caugucauac gagugcccaa ugcuagacga aggagucgag      240

ccagacgacg ucgacugcug guguaacaca accuccacau ggguaguuua uggcacgugu      300

caccacaaaa agggcgaagc ccgccgaagc cgcagagccg ucacccuacc uucacacagc      360

acccgcaagc uacaaacacg cagccaaacc uggcuggaau cacgagaaua cacaaagcac      420

cuaauacgcg ucgagaacug gauauuccgc aaccccggcu ucgcacuagc ggccgcagcg      480

aucgcauggc uccucggcuc cagcacaucg caaaagguca ucuaccucgu aaugauacuc      540

cuaaucgcac ccgcguauag cauacggugu auaggcguca gcaaccgaga cuucguagaa      600

ggcaugagcg gugguacaug ggucgacauc guccucgaac acggaggcug cgucacaguc      660

auggcacaag acaagccaac aguagacauc gaacuuguaa ccaccaccgu uagcaacaug      720

gcugaaguua gaucauacug cuacgaagcc agcauaagcg acauggccuc agacagccga      780

ugcccaacgc aaggagaagc cuaccuagac aaacaaucgg acacccaaua cgucugcaaa      840

cgaacccuag ucgaccgcgg cuggggaaau ggaugcggcc uauucggaaa aggaucccuc      900

gucacaugcg ccaaauucgc gugcucaaag aaaaugaccg gcaaaagcau acaaccagaa      960

aaccuagaau acagaauaau gcucucaguc cacggcagcc aacacagcgg caugaucguc     1020

aacgacacag gccacgaaac cgacgaaaac agagcaaaag ucgagauuac accuaacagu     1080

ccacgcgcug aagccacccu aggcggauuc ggaucgcuag gccucgacug cgaaccacgu     1140

accggacuag acuucuccga ccuauacuac cucacaauga acaacaaaca cuggcucgua     1200

cacaaagaau gguuccacga cauaccccua cccuggcacg ccggagcaga caccgggaca     1260

ccacacugga acaacaaaga agcccucguc gaguucaaag acgcccacgc aaaacgccaa     1320

acagucgucg uauugggcuc ccaagaaggg gcaguccaca cagcccucgc gggagcccua     1380

gaagcagaaa uggacggcgc caaaggacga cuaagcuccg gccaccuuaa gugcagacuc     1440

aaaauggaca aacugcgacu uaaaggaguc ucauacucac ucugcacagc agcuuucaca     1500

uucacaaaaa uaccagcaga aacccuucac ggcacaguga cgguagaagu ccaguacgcc     1560

ggaaccgacg gccccugcaa aguacccgcc caaauggccg uagacaugca aacgcuaacc     1620

ccaguuggac gucucauaac agcuaaccca guaaucaccg aaucgacuga aaacucgaag     1680

augaugcuag aacucgaccc accauucggc gacagcuaca ucguaaucgg cgucggcgag     1740

aaaaagauaa cucaucacug gcaccgcagc gguagcacaa ucggcaaagc cuucgaagcc     1800

acgguaaggg gagcaaaacg cauggcaguc cugggggaca ccgcauggga uuucggaucg     1860

gugggcgggg cccuaaacuc acugggcaaa ggaauccacc agaucuucgg cgccgcuuuc     1920

aaaucacucu ucggcggcau guccugguuc ucacaaauac ucaucggaac acuacucaug     1980

uggcucggac ucaacaccaa aaacggaagc auaagccuca ugugccucgc acuaggagga     2040

guccucauuu uccucucaac cgccguuucc gca                                  2073


<210>  2842
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2842
cgcggccuga gcagcagaaa acgacgauca cacgacguac ucacagucca auuccucaua       60

cucggaaugc uacucaugac cggcgggguc acgagaagag gcagcgcaua cuacauguau      120

uuagaccgaa acgacgccgg cgaagccauc uccuucccca ccacacuggg caugaacaaa      180

ugcuacaucc aaaucaugga ccuaggacac augugcgacg cgaccauguc auacgaaugc      240

ccgaugcucg acgaaggcgu cgaaccagac gacgucgauu gcuggugcaa caccacaucc      300

accuggguag uauacggcac augccaccac aagaaaggag aagcccgacg cagccggcgc      360

gcugucaccc ucccgucaca cucuacccga aagcugcaaa cccgaucaca gaccuggcug      420

gaaucacgcg aauacacaaa acaccucauc cgcgucgaaa acuggauauu ccgcaacccc      480

ggcuucgccc uagcagcugc agccaucgcc uggcuacucg gcuccagcac cagccaaaag      540

gucaucuacc ucguaaugau ccuccucaua gccccagcau acucaauccg augcaucgga      600

gugagcaacc gugacuucgu cgaaggaaug uccggcggga caugggucga uauugugcuc      660

gaacacggag gcugcgucac aguaauggcc caagacaaac caaccguaga cauagagcuc      720

guuaccacca ccguauccaa cauggccgaa gugcgcuccu acugcuacga agcaagcauc      780

agcgacaugg ccuccgauuc aagaugcccc acgcaaggcg aagcauaccu agacaaacaa      840

ucagacacac aauacgucug caaacgaacc cucgucgaca ggggaugggg gaacggcugc      900

ggccuuuuug ggaaaggauc ccuagucacc ugugccaaau uugcaugcag caaaaagaug      960

accggaaaau caauucaacc agaaaaccuc gaauaccgaa uaaugcucuc aguccacggc     1020

agccagcaca gcggaaugau cguaaacgau acaggccacg aaacagacga aaaccgcgcc     1080

aaaguagaaa uaacacccaa cucaccccgc gcagaagcca cacuaggugg auucgggagc     1140

cuaggacuag acugcgaacc ccgcacaggu cuagacuucu ccgaccucua cuaccucaca     1200

augaacaaca agcacuggcu aguccacaaa gaaugguucc acgacauccc gcuaccaugg     1260

cacgccggcg ccgacaccgg aacaccccac uggaacaaca aagaagcccu agucgaguuc     1320

aaagacgcac acgcaaagag acaaacuguc guaguccucg gcucccaaga aggcgccguc     1380

cauaccgcac ucgcaggcgc auuagaagcc gagauggacg gcgcaaaagg acgacuaagc     1440

agcggacacc ucaaaugccg ccuaaaaaug gacaaacuga gacucaaagg agucucuuac     1500

ucacucugca ccgccgcauu caccuucacg aaaauccccg ccgaaacccu ccacgguacc     1560

guuaccgucg aaguccaaua ugcagggaca gacggcccau gcaaaguccc cgcccaaaug     1620

gcagucgaca ugcagacccu cacgccaguc ggcagacuaa ucacagcaaa ccccgucaua     1680

accgaaagca cggaaaacuc caaaaugaug cucgaacuag aucccccauu cggcgacuca     1740

uacaucguca ucggaguagg ugagaaaaag aucacccacc acuggcacag aagcggaagc     1800

acgaucggua aagccuucga agccacuguc cgaggugcca aacgcauggc ugugcucgga     1860

gacaccgccu gggacuucgg gagcgugggc ggagcccuaa acagccuagg gaaaggcaua     1920

caccagaucu ucggcgcagc auucaagagc uuauucggcg ggaugagcug guucucacaa     1980

auccucaucg gaacacuccu cauguggcua ggacucaaca ccaaaaacgg aagcauaagu     2040

cugaugugcc ucgcccucgg aggaguccuu auauuccuau ccacagccgu cagcgcg        2097


<210>  2843
<211>  2097
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant CDS opt

<400>  2843
agaggccuau caagccgcaa aagacgguca cacgacguau uaacaguaca auucuuaauc       60

cuaggcaugc ugcuaaugac aggggcaguu acccgccgag gcucagcgua cuacauguac      120

cuggaccgua acgacgccgg cgaagcaauu agcuucccaa ccacacucgg gaugaacaaa      180

ugcuacaucc aaauaaugga ccuaggacac augugcgacg ccacaauguc guacgaaugu      240

ccaaugcuag acgaaggcgu cgagccagac gacgucgacu gcuggugcaa uacaaccagc      300

acaugggucg uauacggaac cugccaccac aagaaaggcg aagcccgccg aucccgccgc      360

gcaguaaccc uccccagcca cagcacccga aaacuccaaa cccggagcca aacauggcua      420

gagagcaggg aguacacaaa acaccugauc aggguagaga acuggauauu ccgaaacccc      480

ggauucgcac ucgccgccgc agcuaucgcc uggcuacucg gauccuccac cucucaaaag      540

gugaucuauc uuguaaugau ccuccucaua gcacccgccu acucaauacg cugcauagga      600

guuagcaaca gagacuucgu cgaaggcaug agcggcggaa caugggucga caucguccuc      660

gagcacggcg gaugcgucac agucauggcc caggacaaac caaccguaga cauagagcua      720

guaacaacaa caguauccaa uauggccgaa guccgcuccu acugcuacga ggcaucaauc      780

ucagauaugg ccucagacuc gcgaugucca acccaagggg aagccuaccu cgacaagcag      840

agcgacacac aguacguaug uaaaagaacc cuuguugacc gaggcugggg aaacggaugc      900

ggccuauuug guaaaggauc acucguaacc ugugccaaau ucgcaugcuc caagaaaaug      960

accggcaaaa gcauacaacc cgagaaccuc gaauaccgca uaaugcucag cguacacggc     1020

agccaacaca gcgggaugau aguaaacgac accggacacg aaaccgacga aaaucgagcg     1080

aaaguagaaa uaacaccaaa cagcccaaga gccgaagcca cccucggcgg auucggaagc     1140

cucggacuag acugcgagcc gcgcacaggg cucgacuucu ccgaccucua cuaccucaca     1200

augaacaaca aacacuggcu cguccacaaa gaaugguucc acgacauacc acuccccugg     1260

cacgccggcg ccgauaccgg caccccacac uggaacaaca aagaggcccu ggucgaguuc     1320

aaagacgcgc acgccaagcg ccaaacagug gucguacucg gaucacaaga aggcgcagua     1380

cacacagccc ucgccggagc ccucgaagcc gaaauggacg gcgccaaagg acgccuaagc     1440

uccggccacc ucaagugccg ccucaaaaug gacaaauugc gacucaaggg agucagcuac     1500

ucacugugca cagccgccuu cacauucaca aaaauccccg ccgaaacacu gcacggaaca     1560

gucacaguag aaguacaaua cgcaggcacu gacggaccau guaaaguacc ggcgcaaaug     1620

gcagucgaca ugcaaacccu cacgccaguc ggacgacuaa ucaccgccaa uccagucauc     1680

acagaaagca cagaaaacuc aaaaaugaug cuagaacuag aucccccauu cggagacagc     1740

uacaucguga uaggcgucgg cgaaaagaaa aucacccacc acuggcaccg aagcggcuca     1800

accauaggca aagcguucga agccaccguc agaggcgcua aacggauggc aguacucggc     1860

gacacggcau gggacuucgg uucagucggg ggcgcgcuaa acagccucgg caaaggaauc     1920

caccaaauuu ucggggccgc auucaaaagc cucuucggcg gaaugaguug guucucccaa     1980

auacuaaucg gcacccuccu gauguggcua ggccuaaaca ccaaaaacgg uucgaucagc     2040

cucaugugcc uagcguuggg aggcgugcua auauuccuaa gcacagccgu aagugcc        2097


<210>  2844
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long CDS opt

<400>  2844
cgaauaauaa acgcaagaaa ggaaaagaaa cggcgcggcg cagacacauc cgucggaaua       60

gugggacucc uacucacaac cgcaauggcc gcggaaguaa caagaagagg cucagcguac      120

uacauguacc uagaccgcaa cgacgcagga gaagcaauau ccuuccccac aacccucggc      180

augaacaaau gcuacauaca gaucauggac cucggacaca ugugcgacgc aaccaugagc      240

uacgaaugcc caaugcuaga ugaaggcguc gaacccgacg acgucgacug cuggugcaac      300

accaccucca cguggguagu cuauggaacc ugccaccaca agaaaggcga agcccgaaga      360

ucaaggagag cagucacacu accaagccac agcacacgaa aacuacaaac acguucacaa      420

accuggcucg aauccagaga auacacaaaa caccucauac gagucgaaaa cuggauauuc      480

agaaaccccg gcuucgcccu cgcggcagcc gcgauagcau ggcugcuggg aagcaguacc      540

ucacaaaaag ucauauaccu aguaaugauc cuccucaucg cgcccgccua cagcauucgc      600

ugcauaggug uaagcaaccg ggacuucgua gaagggauga gcggcgguac augggucgac      660

aucguacuag aacacggggg cugcguuaca guaauggcac aagauaagcc caccguagac      720

auagaacucg ucacaacaac cguaucaaac auggcugagg ucagaagcua cugcuacgaa      780

gcauccaucu ccgacauggc aagugacagu cgcugcccca cccaaggaga agcguaccuc      840

gacaaacaau ccgacaccca auacgucugc aaacgaacac ucguagaccg cggaugggga      900

aauggcugug gacuuuucgg aaaaggcagc cuaguaacau gcgccaaguu cgccugcagc      960

aagaaaauga caggcaaauc aauccaacca gaaaaucucg aguaccgcau uauguuauca     1020

guacacggca gccaacacag cggcaugauc gucaacgaua ccgggcacga aaccgacgaa     1080

aacagagcca aaguagaaau cacaccaaac agcccaagag cagaagccac acuugggggc     1140

uuuggcagcc uaggccucga cugcgaacca cgaacaggcc ucgacuucuc agaucuauac     1200

uaccucacca ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacaucccc     1260

cuccccuggc augccggggc agacacggga acaccccauu ggaacaauaa agaagcccua     1320

guagaauuua aagacgccca cgccaaaaga caaaccgucg ucguccucgg cagccaagaa     1380

ggggccgucc acaccgcucu agccggggcc cucgaagcag agauggacgg agccaaggga     1440

cggcuaagcu ccggccaccu aaaaugcaga cucaagaugg acaaacuaag gcucaagggg     1500

gucucauaca gcuuaugcac agccgcauuc acauucacca aaaucccggc agagacauua     1560

cacggcacag ucaccgucga aguacaauac gcaggcacag acggcccaug caaaguccca     1620

gcccaaaugg cgguagacau gcaaacacuc acccccgucg gccgccuuau cacagccaac     1680

ccagucauca ccgaaagcac ggaaaacagc aagaugaugc ucgaacucga cccacccuuc     1740

ggcgacucgu acauaguaau cggagucgga gagaaaaaga uaacccauca cuggcaccgc     1800

agcggcucca ccauaggcaa agccuucgaa gcgaccguac gcggcgcaaa acggauggcc     1860

guacucggcg acacugccug ggacuucgga ucaguaggcg gcgcccucaa cucacuaggc     1920

aagggaaucc accaaaucuu uggcgcagcc uucaaaagcc uauucggcgg aaugucgugg     1980

uucagccaaa uccucauagg cacguuacuc auguggcucg gccuaaacac caaaaacggc     2040

uccaucuccc uaaugugccu cgccuuaggc ggaguccuga ucuuccucag cacagcagua     2100

ucggccgacg uaggaugcuc cguagacuuc agcaaa                               2136


<210>  2845
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long CDS opt

<400>  2845
agaauaauaa acgcgcgcaa agaaaagaaa cgacgcggcg cagacacaag ugucggaauc       60

gucggccucc ugcugacgac agccauggcu gcagaaguca cacgcagagg aagcgccuac      120

uacauguacc uagaccgaaa cgacgcaggc gaagccauau cauucccaac aacgcucggc      180

augaacaaau gcuacauuca aauaauggac cuagggcaca caugcgacgc aacgaugagc      240

uacgaaugcc ccaugcucga cgaaggaguc gaaccagacg acguagacug cuggugcaac      300

acgaccagca cguggguggu auacggaacc ugccaccaca agaaagggga agcacgaaga      360

ucacgacgcg ccgugacccu ccccagucac agcacacgaa aacuccaaac acgcucccaa      420

acauggcuag aaucacgcga guacacgaaa caccucaucc gagucgaaaa cuggaucuuu      480

agaaacccag gcuuugcacu cgcagcggcc gccauugcau ggcuacuggg caguagcaca      540

ucccagaaag uuauauaccu cguaaugaua cuucuaauag cgcccgcaua cuccauccgc      600

ugcauaggag uguccaauag agacuucgua gaaggaauga gcggaggcac cuggguagac      660

guagucuuag aacacggagg cugcgucaca gucauggccc aagacaaacc gaccguagau      720

auagaacucg uaaccaccac agucagcaac auggcagaag ugcgaucaua cugcuacgaa      780

gccucaaucu ccgacauggc cagcgacagc cggugcccaa cacaaggaga agccuaccuc      840

gacaaacaau cagacaccca auaugucugu aaacgcacac ucgucgacag agggugggga      900

aacggcugcg gccucuucgg caaaggguca cucgucacgu gcgcaaaauu cgcaugcagc      960

aagaaaauga cgggcaaauc cauccaaccc gaaaaccucg aauaccgaau aaugcucagc     1020

guccacgggu cacaacacag cggaaugaua gucaaugaca ccggacacga aacagacgaa     1080

aacagagcaa aaguagaaau cacaccaaac ucaccgcgag ccgaagccac acugggagga     1140

uucgggaguc ucggccucga cugcgaaccc cggacuggcc uagacuucuc cgaccuauac     1200

uaccuaacga ugaacaacaa acauuggcuc guccacaaag aaugguucca cgacauccca     1260

cucccguggc acgccggcgc cgacaccggc acuccccacu ggaacaacaa agaagcccuu     1320

gucgaguuca aagacgccca cgcaaaacgg caaacaguag uagucuuagg gucacaggag     1380

ggcgccguuc acaccgcccu cgcuggggcg cuugaggccg aaauggacgg cgccaaaggc     1440

cgccucucca gcggacaccu gaaaugccga cuuaaaaugg acaaacuacg acuaaaagga     1500

gucuccuaca gccucugcac agcagccuuc accuucacga agaucccagc cgaaacacua     1560

cacggaacag ucacaguaga aguacaauac gcagggaccg acggcccaug caaagucccc     1620

gcccaaaugg ccguagacau gcagacacua acgcccguag gaagacucau cacagcaaac     1680

ccgguaauca ccgaaagcac cgaaaacucc aaaaugaugc uggaacucga ucccccauuc     1740

ggagacagcu acauagucau cggaguagga gaaaagaaaa uaacacacca cuggcaccgc     1800

agcggaucaa ccauaggaaa agccuuugaa gccaccguaa gaggcgcaaa aaggauggcg     1860

guucuggggg acacagcaug ggacuucggc uccguaggcg gcgcacuaaa cucccucggc     1920

aaaggaauac accaaaucuu cggagcagcc uucaaaucuc ucuucggcgg aaugagcugg     1980

uucagccaaa uccuaauagg cacgcuacuc auguggcuag gacuaaacgc caagaacgga     2040

uccaucagcc uaaugugccu ggcucucggc ggcguguuaa uauuccuuag cacagcaguc     2100

uccgccgacg uaggcugcag cguagacuuc uccaaa                               2136


<210>  2846
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long CDS opt

<400>  2846
cgcaucauaa acgcgcgcaa agaacgcaaa cgaagaggcg cagauacaag cauaggaaua       60

aucggacucc ugcucacaac agcgauggca gcagagauca cacgaagggg gucagccuac      120

uacauguacc ucgacagguc cgacgcagga aaagcaauau cauucgcaac cacacuagga      180

gucaacaaau gccacguaca aaucauggac cuaggacaca ugugcgacgc cacaaugucg      240

uacgaaugcc cgaugcugga cgaaggagug gaaccggacg acguagacug cuggugcaac      300

acaacaagua cguggguggu cuacggaacg ugccaccaca agaaaggaga agcccgacga      360

agcagacgag caguaacacu gcccagccau ucaacaagaa aauugcaaac cagaucccag      420

acauggcugg aaagcaggga auacaccaaa caccucauaa agguagaaaa cuggauauuc      480

cgaaaucccg gauucgcacu cguagccgua gcaauagccu gguuacuggg cucaagcaca      540

agccaaaaag uaauauaccu agugaugaua cuccugaucg cccccgcgua cucgauccga      600

ugcauaggag ucagcaaccg ggacuuuguu gaagggaugu caggaggaac auggguagac      660

gucgugcucg aacacggagg cugugugaca guaauggcgc aagacaaacc cacggucgac      720

auagagcuag uaacaacaac uguaagcaac auggcagaag ugagaagcua cugcuacgaa      780

gccucgaucu cagacauggc aagcgauagc cgaugcccua cgcaaggaga agccuaccug      840

gacaaacagu cagacaccca guacgucugc aaacggacac uggucgaccg cggcuggggc      900

aacggcugcg gccucuucgg gaagggauca cucgugaccu gcgcaaaguu cacgugcagc      960

aagaaaauga ccggcaaaag cauacaacca gaaaaccuag aauaccgaau caugcucuca     1020

guacacggcu cgcaacacuc aggaaugauc ggauacgaaa cagacgaaga ccgagcaaaa     1080

guagaaguca cuccaaacag cccgcgcgca gaagcaacac uagggggcuu cgggagccuc     1140

gggcuagacu gugaaccacg cacgggacug gacuucagcg accucuauua ccuaacgaug     1200

aacaacaaac acuggcuggu ccacaaagaa ugguuccacg acaucccacu uccguggcac     1260

gccggagccg acaccggcac accccacugg aacaacaaag aagcccuagu cgaguucaag     1320

gacgcccacg cuaaaagaca aaccgucguc guccucggcu cacaagaagg agcgguccac     1380

accgcgcucg cgggcgcucu cgaagcggag auggacggug ccaaaggacg cuuauucucc     1440

gggcaccuaa agugcaggcu aaaaauggac aaauuaagac ucaaaggagu auccuacucg     1500

cucuguaccg cagccuuuac cuucacaaag gucccagccg aaacccuaca cgggacagug     1560

acaguugaag uccaguacgc cggcacagac ggaccaugca aaaucccggu acagauggcg     1620

guagacaugc aaacauugac accaguggga cgccucauaa cugcuaaccc aguaauaaca     1680

gaaucaaccg aaaacaguaa aaugaugcug gagcuggacc cgccauucgg agacagcuac     1740

aucgugauag gaguaggaga caagaaaaua acacaccacu ggcacaggag cggcagcacc     1800

aucggaaaag cauuugaagc gacagucagg ggcgcgaaaa gaauggcagu acuaggggac     1860

accgcauggg acuuugggag cgugggagga guuuuuaaca gcuuaggaaa aggcauccac     1920

cagaucuucg gagccgcauu caagagcuug uucggaggca ugagcugguu cagccaaaua     1980

cuaauaggga cccuccuggu cuggcuuggg cugaacacca aaaacggaag caucagccug     2040

acaugccucg cacuaggagg cguaaugaua uuccuaagca cagccgugag cgcggacgug     2100

gggugcagcg uggacuucag caaa                                            2124


<210>  2847
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long CDS opt

<400>  2847
cgaaucauaa acgcacggaa agaaaagaag agaagaggag cagacaccag cguagggauc       60

gugggccugc uccucacaac ggccauggcc gccgaaguca cccgcagagg aucagcuuac      120

uacauguacc uagaccgcaa ugacgcgggc gaagcaauca gcuuccccac gacccuaggc      180

augaacaaau gcuacauaca aauaauggac uuaggccaca ugugcgacgc aaccaugagu      240

uacgaaugcc cgaugcugga cgaagggguc gaaccagacg acguagacug uugguguaac      300

accacaucaa cauggguggu guacgggacc ugccaccaca agaaaggcga agccaggcga      360

ucgagacgag cuguaacccu accaagccac agcaccagaa aacuacaaac gagaucacaa      420

acguggcugg agucacgcga auacaccaaa caccucaucc gaguugagaa cuggauauuc      480

cgaaacccgg guuucgcauu ggcagcggcc gccaucgccu gguuacucgg aagcucuacg      540

agccaaaagg ucaucuaccu aguaaugauc cuccucauag caccagcaua cagcauccgc      600

uguauuggcg ucagcaacag ggacuucgua gaaggcauga gcgggggcac augggucgac      660

guaguacuug aacacggcgg cugcgucacc guaauggcac aagacaaacc cacagucgac      720

aucgaacucg uaacaacgac cguaagcaac auggcggaag ugcgaagcua cugcuacgaa      780

gcaucaauca gcgacauggc gagcgacagc agaugcccua cccagggcga agcauaccuc      840

gacaaacaaa gcgacacaca auacguaugc aagagaacuc uagucgaccg aggguggggc      900

aacggaugcg gacuauucgg aaaaggcagc cucgucacau gugcaaaauu cgccugcuca      960

aagaaaauga cagguaaaag cauacaaccc gaaaaccugg aguaccgcau aaugcuaagc     1020

guacacgggu cccaacacag cggcaugaua gucaacgaca ccggacacga gacagacgaa     1080

aaccgagcca aaguggaaau aaccccaaac agcccaaggg cagaggccac ccuaggaggg     1140

uucggcagcc ucggccucga cugcgaacca cgcaccggcc uagacuucuc agaccuauac     1200

uaccucacaa ugaacaacaa gcacuggcuc guacacaaag aaugguucca cgacaucccc     1260

cuuccauggc acgcgggagc cgacaccgga acgccccacu ggaacaacaa ggaagcgcuc     1320

guggaguuca aagacgccca cgcaaaacga caaaccgugg uaguccuagg aagccaagaa     1380

ggggcaguac acaccgcacu cgccggagca cuagaagcgg aaauggacgg agcgaaaggg     1440

agacucagca gcggacaccu gaaaugucga cucaaaaugg acaagcuacg gcugaaggga     1500

gugagcuauu cccucugcac agcagccuuc acauucacaa aaaucccggc cgaaacacua     1560

cacggaaccg ucacugugga gguacaauac gccggcacag acggccccug caagguacca     1620

gcccaaaugg caguagacau gcaaacacuc acaccagugg gccgccuaau aacggcaaau     1680

ccaguuauaa ccgagagcac ggaaaacucc aagaugaugc ucgaacucga ucccccguuc     1740

ggagacucau acauaguaau aggcguaggg gagaaaaaga ucacccacca cuggcacaga     1800

uccggcucga caauaggaaa agccuucgaa gccaccguac gcggagcaaa aagaauggcg     1860

guacuaggag acacagcaug ggacuucgga uccgugggcg gagcccuaaa cucccucgga     1920

aagggaauac accagauauu cggagcggca uucaaauccc uauucggagg aaugagcugg     1980

uucagccaga uccucauagg aacacuacua auguggcuag gucucaacac caaaaacgga     2040

agcauuagcc ucaugugccu cgcacuaggc gguguacuaa uuuuccuaag cacagccgua     2100

ucagcggacg uaggaugcag cguagacuuc uccaag                               2136


<210>  2848
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long CDS opt

<400>  2848
cggaucauaa acgccaggaa agaacgaaaa cggagaggag cagacacgag cauuggcauc       60

aucgggcuac uacuaacgac cgcgauggcg gcggagauca cacgaagagg aagcgcauac      120

uacauguacc uggacagguc cgacgccgga aaagccauuu cauuugccac aacacuaggg      180

guaaauaagu gccacguaca aauaauggau cuuggacaca ugugcgacgc aacaaugucc      240

uacgaaugcc caaugcugga ugagggcgua gaaccagacg acgucgacug cuggugcaac      300

acgacaucga cgugggucgu auacggaaca ugccaccaca agaaggggga agcacgaaga      360

ucgcggagag caguaacacu cccgagucac ucgacacgga agcuacaaac aaggagccaa      420

acauggcugg aguccagaga auacacaaag caccuaauaa aagucgaaaa cuggaucuuc      480

cgcaaccccg guuuugcccu agucgcaguc gcaauagccu ggcuacuagg cucaagcacc      540

agccagaaag uaauauaucu agugaugaua cuccugauag cgccagcgua cagcauuagg      600

ugcauaggag uaagcaaccg cgacuucgua gaaggcaugu cgggcgggac cugggucgac      660

gucguauuag aacacggagg cugcgucacc guaauggcac aggacaaacc aacaguagac      720

aucgaacucg uaaccaccac agucagcaac auggcggagg ucagaagcua cuguuacgag      780

gcaucuauaa gugacauggc cagugacagc cgcuguccaa cacaaggaga agcauaccua      840

gacaaacaau cagacacaca auacgucugc aagagaaccc uaguagaccg gggauggggg      900

aacgggugcg gacuuuucgg caagggcucg cucguaacau gcgcaaaauu cacgugcucg      960

aagaaaauga cgggcaaaag cauccaacca gaaaaccucg aauacaggau aaugcuaucg     1020

gugcacggcu cacaacacuc aggaaugaua guaaacgaca ccggauauga aacagacgaa     1080

aaccgggcaa agguagaggu aacaccgaac agcccccggg cagaggcgac acuaggcggc     1140

uucggaagcc uaggcuuaga cugcgaacca agaacaggcc ucgacuucuc agaccucuac     1200

uaccuaacca ugaacaacaa acacuggcuc guccacaaag agugguucca cgacauaccg     1260

cucccauggc acgcgggagc cgacacagga acaccgcacu ggaacaacaa agaggcccua     1320

guagaguuca aagacgcaca cgcaaaaaga caaacaguag uagugcuagg cagccaagaa     1380

ggcgccgugc acacagcccu cgccggagcg cuggaagccg aaauggacgg cgcgaaaggc     1440

aaacuguucu ccggacaccu aaagugccgg cucaaaaugg acaagcugcg acucaagggc     1500

guaagcuacu cccuguguac agcagccuuc acauucacaa aagugccggc cgagacccua     1560

cacgggaccg ugacaguaga agugcaauac gccggaacag acggccccug caagaucccg     1620

guacaaaugg cagucgauau gcaaacacua acacccgucg ggagauugau aaccgcaaac     1680

cccguaauaa cagaaaguac agagaacagu aaaaugaugc uagaacugga cccgcccuuc     1740

ggcgacagcu acauagucau cggcgucgga gauaagaaaa ucacacacca cuggcaccga     1800

agcgggagca caauagggaa agcguucgag gcaacaguca ggggagcgaa acggauggcc     1860

guacuaggcg auaccgcgug ggacuucgga agugucggcg gcguauucaa cucacuaggg     1920

aaaggaaucc accaaaucuu cggagcagcc uucaagagcc uauucggcgg caugaguugg     1980

uucucacaaa uacugauagg cacacugcug guauggcuag ggcuaaauac caaaaacggg     2040

agcauaagcc uaaccugccu cgcacucggu ggcgugauga ucuuccugag cacagccgua     2100

agcgcggacg uagggugcag cguggacuuu agcaaa                               2136


<210>  2849
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long CDS opt

<400>  2849
agaaucauca acgccaggaa agagcgaaaa cgucguggag cagacacauc gauaggcaua       60

gucggccuac ugcucacaac cgcaauggcc gcagagauaa cacgcagagg cucagcauac      120

uacauguacc ucgaccgaag ugacgccgga aaagcaaucu cauucgcaac aacacuaggc      180

guaaacaaau gccacguaca aauuauggac cuaggucaca ugugcgacgc caccauguca      240

uacgagugcc cgaugcuaga cgaaggcgug gaaccagacg acguagacug cuggugcaac      300

acaacgagca ccugggucgu auacgggacg ugccaccaca agaaaggcga agcaagacga      360

agccggcgcg ccgugacacu cccaagccac agcacccgaa aacuacagac cagaagccag      420

acuuggcuag aaagccgaga auacaccaaa caccucauca aaguagaaaa cuggaucuuc      480

agaaacccag gguucacgcu cguagccgua gccauagccu ggcucuuagg cucaagcacg      540

ucgcaaaaag ucaucuaccu agugaugaua cuccugauag cgcccgccua cagcauacga      600

ugcaucggag ucagcaaccg cgacuucgug gagggcaugu caggagggac cugggucgac      660

guaguccugg aacacggagg cuguguaaca guaauggccc aggacaaacc aacagucgac      720

aucgaacucg uaaccaccac cguguccaac auggcagaag ucagaucuua cugcuacgaa      780

gccagcaucu cagacauggc cucagacuca cgcuguccca cacaaggaga agcguacuua      840

gacaaacaau cagacaccca auacguaugc aaacgcacgc ucgucgaucg agguugggga      900

aacggcugcg gacuauucgg caaaggcucg cucguaaccu gugcaaaauu cacaugcagc      960

aagaaaauga caggcaaaag cauccaaccg gaaaaccuag aauaccggau caugcucucg     1020

guacacggcu cccaacacag cggaaugaua gucaacgacg agaaccgcgc aaaaguagaa     1080

gucaccccaa acucuccccg cgcagaagca acccuaggag gguucggauc ccuaggcuug     1140

gacugcgaac ccagaacagg acucgacuuc agcgaccucu acuaccuaac aaugaacaac     1200

aagcacuggu ugguucacaa agaaugguuc cacgacaucc cucucccaug gcaugcgggg     1260

gcagacacag ggacgccuca cuggaacaac aaagaagccc uagucgaguu caaagacgcg     1320

cacgcaaaac gccaaacggu cguaguacuc ggcagccaag aaggcgcggu ccacaccgcg     1380

cuagccggcg cucucgaagc ggaaauggac ggagcaaaag gacgccuauu cuccggccac     1440

cucaagugcc gacuaaagau ggacaaacua cgauugaaag ggguauccua uucccucugc     1500

acagcagcau ucaccuucac caaggucccc gccgaaaccc uacacggcac cgucaccguc     1560

gaagugcaau acgcaggaac cgacggcccg ugcaaagucc ccgcacaaau ggccgucgau     1620

augcagacac uaacaccggu aggacgacug aucacugcga accccgugau caccgaaagc     1680

accgaaaacu caaaaaugau gcucgaacua gacccuccgu ucggcgacag cuacaucguc     1740

aucggggucg gcgacaagaa aauaacccac cacuggcacc gcagcggauc aacuaucggc     1800

aaagcguucg aggccaccgu aagaggcgcc aaacggaugg caguacuagg cgauacagca     1860

ugggauuucg gguccguagg aggcgucuuc aacagccucg gaaaaggcau ccaccaaaua     1920

uucggcgccg cguucaagag ccuauuuggc ggaaugagcu gguucucaca aauccugauc     1980

ggaacucucc uagucuggcu aggacuaaac acgaaaaaug gaucaauaag ccuaacaugc     2040

cuggcacuag gaggcgucau gauauuccua aguacagcag ucagcgccga cgucggaugc     2100

agcguagacu ucagcaaa                                                   2118


<210>  2850
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long CDS opt

<400>  2850
cgcaucauaa acgcgagaaa agaacgaaaa cgccgcgggg cggacacgag caucggcaua       60

aucggacugu ugcuaacgac cgcgauggcc gcggagauca caagacgagg cagcgcguac      120

uauauguacc ucgaccgguc cgacgccgga aaagccaucu cguucgccac cacccuagga      180

gucaacaaau gccacgucca aauaauggac cucggccaca ugugcgaugc cacaauguca      240

uacgagugcc ccaugcuaga cgaaggugua gaaccagacg acguagacug cuggugcaau      300

accacgagca cguggguagu uuacggcacg ugucacagaa agaaaggaga agccaggaga      360

ucaagacgcg cggucacccu cccaagccac ucaacacgca aauuacagac cagaagccag      420

accuggcucg aguccagaga auacaccaaa caccugauaa aagucgaaaa cuggaucuuu      480

cgcaaccccg gauucgcacu cguggcagua gcgauagcau ggcuccuagg cagcagcaca      540

ucccaaaaag ugauuuaucu cgucaugauc cuacugauag caccggcgua cagcaucagg      600

ugcauaggag uaaguaacag ggauuuugua gaaggcaugu cuggcggaac guggguagac      660

gucguacucg agcacggagg cugugucaca gucauggccc aagacaaacc cacagucgac      720

aucgagcucg ucaccacgac agucagcaac auggcagaag ugcgaucaua cugcuacgaa      780

gcgucgauca gcgacauggc auccgacucg cgcugcccca cgcaagguga agcauaccua      840

gacaagcaau cagacacaca auaugucugc aaacgcaccc ucgucgaccg aggauggggc      900

aacggaugcg gacucuuugg aaaaggaucc cuaguaaccu gcgccaaauu uaccugcagc      960

aaaaagauga cgggcaagag cauccaaccc gaaaaccucg aguacagaau caugcugucc     1020

guccacggcu cgcaacacuc aggaaugaua gucaacgaca caggcuacga aacagaugaa     1080

aacagggcaa aagucgaagu cacccccaac uccccacgcg cggaagccac ccugggcgga     1140

uucggcuccc uaggccuaga cugcgaacca cgcacaggac ucgacuucag cgaccuguac     1200

uaccuaacaa ugaacaacaa acacuggcua guccacaagg agugguucca cgacauacca     1260

cuccccuggc acgcaggcgc cgacaccgga accccgcacu ggaacaacaa agaagcacuc     1320

gucgaguuca aagacgcaca cgcaaagaga caaacgguag uaguccuggg aucccaagaa     1380

ggcgcaguac acacagcacu ggccggcgcc cuagaagccg aaauggacgg cgcgaaagga     1440

aaacuguuca gcggccaccu caaaugccgc cuaaaaaugg acaaacuacg acucaaagga     1500

guaucuuacu cccuauguac cgcagccuuc accuucacca aaguaccggc cgaaacgcuc     1560

cacggaacug ugacagucga gguacaauac gccggcaccg acggacccug caaaauacca     1620

guacaaaugg ccguagacau gcaaacgcua acgcccguag gacggcuaau cacagcgaac     1680

cccgugauaa cagaaucaac cgagaacuca aaaaugaugc ucgaacuaga cccgcccuuc     1740

ggcgacagcu auauagugau aggcguaggc gacaagaaaa uaacccacca cuggcacaga     1800

agcggaucca ccaucggcaa agcguucgaa gcaaccguaa ggggcgccaa aagaauggca     1860

guccuaggag auaccgccug ggacuucgga agcgucggag gagucuucaa cagccuugga     1920

aaagggauuc accaaaucuu cggagccgcc uucaaaagcc uauucggggg cauguccugg     1980

uucagccaaa uacuaaucgg cacccuauua gucuggcucg gccucaauac caaaaauggc     2040

agcauuagcc uaaccugccu agcacucgga ggcgucauga ucuuccugag cacagccgua     2100

agcgccgacg uaggcugcuc cguagacuuc uccaaa                               2136


<210>  2851
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long CDS opt

<400>  2851
cgcauaauca acgccagaaa agaacggaaa cgacgaggcg cggacacauc aauaggaauc       60

auaggacugc ugcugacaac agcgauggcg gccgagauaa caagacgagg cucggccuac      120

uacauguacc ucgaccggag cgacgcaggg aaagcgauau cauucgcaac gacacucgga      180

guaaacaagu gccacguaca aaucauggac cucggacaca ugugcgacgc cacgauguca      240

uacgagugcc ccaugcugga cgagggcguc gaaccagacg acguagacug cuggugcaac      300

accacaagca cauggguagu auacggaacc ugccaccaca agaaaggcga agcgcgaagg      360

ucaagacgag cagucacccu accaagccac agcacaagaa aacuccaaac ccgcucacaa      420

accuggcuag aaagccgaga auauaccaaa caccucauca aaguagaaaa cuggauauuc      480

cgcaacccag gcuucgcacu cguggcagua gccauagcau gguuauuagg caguagcacu      540

agccaaaagg uaaucuaccu agucaugaua cuccuaaucg cgcccgcgua cucaaucaga      600

ugcaucggag uaagcaacag agacuucgua gaagggaugu cagggggaac auggguagac      660

gucguacuag aacacggagg augcgucacg guaauggccc aggacaagcc aaccgucgac      720

auagagcucg ugaccacuac cguaagcaac auggcggaag uacgaucaua uugcuacgaa      780

gccucaauau cagacauggc cucugacagc cgcugcccaa cacaaggaga agcguaccua      840

gacaagcaaa gcgacacgca auacgucugc aaacggacac ucguagaccg cggaugggga      900

aacggcugcg gucuauucgg aaagggcucg cucguaaccu gcgcaaaauu uaccugcuca      960

aagaaaauga caggcaaauc aauucagccg gaaaaccucg aguacaggau caugcucucc     1020

guacacggcu cacaacauag cggcaugaca gucaacgaca uaggauacga aacugacgaa     1080

aacagggcca aagucgaagu aacgcccaac agcccaagag cagaagccac acugggagga     1140

uucggcagcu uaggccugga cugcgaaccg cgaaccggau uggacuucuc agaccucuac     1200

uaccuaacca ugaacaacaa acacuggcua guccacaaag aaugguucca cgacauacca     1260

cuaccguggc acgcaggcgc agacaccggg accccgcacu ggaacaacaa agaagcgcug     1320

gucgaguuca aagacgcgca ugccaaacga caaaccgucg uggucuuagg aucccaagaa     1380

ggcgcggucc acacggcacu ggcaggcgcu cucgaagccg aaauggacgg agcaaaagga     1440

aagcuauuca gcggccaccu caaaugcaga cucaaaaugg auaaacugag gcucaaaggg     1500

guaucguacu cccugugcac agcagccuuc accuucacaa aaguccccgc agagacccuc     1560

cacggcacag ugacagugga aguccaauac gccgggacag acggaccaug caaaauccca     1620

guccagaugg caguagauau gcaaacacug acacccguag gaagacuaau cacggcaaac     1680

cccgugauaa cagaaucgac agaaaacucc aaaaugaugc uggaauuaga cccacccuuc     1740

ggcgauagcu acaucguaau aggagucggg gacaagaaaa uaacacacca cuggcaccgc     1800

agcggaagca ccaucggcaa agcauucgag gcgacgguca gaggcgcaaa acgaauggcg     1860

gucuuaggag acacagcaug ggacuucggg uccgugggag ggguguucaa cucacuagga     1920

aaaggaaucc aucaaaucuu cggagccgca uucaaaagcc uauucggagg gauguccugg     1980

uucucacaaa uacuaauagg cacacugcug guauggcuag gacucaacac caaaaacggg     2040

agcauaagcc uaaccugccu cgcacuaggg ggaguuauga ucuucuugag cacggccguc     2100

agcgcagacg ucggcugcuc aguagacuuc agcaaa                               2136


<210>  2852
<211>  2124
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long CDS opt

<400>  2852
cgcauaauca acgcccgcaa agaacgcaaa cgaagaggcg cagacacaag cauaggcauc       60

aucggccucc uccucacaac cgccauggca gcagaaauaa cccgcagagg aagcgccuac      120

uacauguacc uagaccgcuc cgacgcaggc aaagccauau cauucgcaac aacccuggga      180

guaaacaaau gccacgucca aauaauggac cugggccaca ugugcgacgc cacgaugucc      240

uacgaaugcc caauguuaga cgaaggagua gaacccgacg acgucgacug cugguguaac      300

acaacuagca cauggguagu cuauggaacc ugccaccaca agaaaggaga agcaagacga      360

ucccgccgcg cggugacccu gccaagccac ucaacacgaa aacuacaaac cagaucccaa      420

accuggcucg aaucacguga auacaccaaa caccucauaa aggucgagaa uuggauauuc      480

cgaaacccag gcuucgcccu aguagcagua gcaauagcau ggcugcucgg gaguagcacc      540

agccaaaagg ucaucuaccu agucaugaua cuacucauag ccccagcaua cagcauccga      600

ugcauaggag uauccaaccg cgacuucgua gaagggauga gcggaggcac cuggguagac      660

guaguccucg aacauggagg augcguaacg guaauggcac aggacaagcc cacggucgac      720

auagaauuag ugacgaccac aguauccaac auggccgagg uacgaaguua cugcuacgaa      780

gcgucaauau ccgacauggc gagugacucg cgcugcccca cacaagggga ggcuuaccuc      840

gacaaacaga gcgacacaca auacgucugc aaacgaaccc uagucgaccg cggauggggu      900

aacggcugcg gacuauuugg caaaggcagc cuagugaccu gcgccaaauu cacauguucc      960

aaaaagauga cuggaaaauc cauacaacca gaaaaccucg aauaccgcau caugcuaucc     1020

guacacggcu cacaacauuc aggcaugaua ggguacgaaa cagacgaaaa ccgcgccaaa     1080

guagaaguca caccaaacag cccaagagca gaggccacac uaggaggcuu cggaucccuc     1140

ggacucgacu gcgagccgcg aaccggccuc gacuucucgg accuauacua ccucacaaug     1200

aacaacaaac acuggcucgu ccacaaagaa ugguuccacg acaucccccu gccauggcac     1260

gcaggcgcag acaccggaac accgcacugg aacaacaaag aagcacuggu agaguucaaa     1320

gacgcccaug ccaaacgcca aacaguagua guucugggau cccaagaagg cgcaguccac     1380

acugcccucg cgggcgcccu cgaagcagaa auggacggcg cuaaaggcaa acuauucucc     1440

gggcaccuca aaugucgacu aaaaauggac aaacuccgac uaaaaggcgu aagcuacucu     1500

cucugcaccg ccgcguucac guucacaaaa guccccgccg aaacacucca cggcacaguc     1560

accgucgaag uccaauacgc cggaaccgac ggacccugca aaauacccgu gcagauggca     1620

guagacaugc aaaccuuaac accagucgga cgccucauaa ccgcaaaccc agucaucaca     1680

gaaagcaccg aaaacuccaa aaugaugcua gaacucgacc cgcccuucgg cgacuccuac     1740

aucgugauag gcguggggga caagaaaauc acacaccacu ggcaccgaag cggcagcaca     1800

aucggaaaag ccuucgaggc cacagucaga ggcgcaaaaa gaauggccgu gcuaggagac     1860

accgcauggg acuucgguuc cguaggcggc guauucaaca gccucggcaa gggaauccac     1920

caaaucuucg gcgcggcuuu caaaagccuc uucggcggca uguccugguu cucacagauc     1980

cugaucggca cacuccucgu cuggcuaggc cuaaacacca aaaacggcuc gauaaguuua     2040

acaugccucg cacugggcgg agucaugauc uuccucucaa ccgcaguuag cgccgacgua     2100

ggaugcagcg uagauuucuc caaa                                            2124


<210>  2853
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long CDS opt

<400>  2853
agaaucauca acgcacgaaa ggaaaagaaa cgcagaggag cagacacaag cgucggcaua       60

guaggacucc uacuaacaac agccauggcc gcagaaguca caagaagggg aagcgcauau      120

uacauguacc ucgaccgcaa cgacgcuggc gaagccauca gcuucccgac cacccuaggc      180

augaacaaau gcuauauaca aauaauggac cucggccaua ugugcgacgc aacaaugagc      240

uacgaaugcc ccaugcucga cgaaggcguc gaaccagacg acgucgacug cuggugcaac      300

acaaccucaa caugggucgu cuacggcacg ugccaccaca aaaagggaga agccagacgc      360

ucccgacgag ccguuacccu cccuagccac agcacgagaa aacuccaaac acgaucacaa      420

accuggcuug aaagccgcga auacaccaaa caccucaucc gcguggaaaa cuggauauuc      480

cgaaaucccg gcuucgcccu cgcagcagcc gcgaucgcau ggcuccuugg cuccuccacg      540

ucccagaaag ucaucuaucu cgucaugauc cuacuuaucg cgccugcaua cuccauaaga      600

ugcaucggcg uaagcaacag agacuucguc gaaggcaugu ccggcggcac cugggucgac      660

gucguccuag aacacggggg uugcguaacc gucauggcuc aggacaaacc aacagucgac      720

aucgaacugg uaacaacgac cgucagcaac auggccgaag uacgcagcua cugcuaugag      780

gcuucaauaa gcgacauggc cagcgacagc cggugcccaa cacaaggaga agccuaccua      840

gacaaacaau cagacaccca auacgucugu aagagaaccc ugguggaccg cggaugggga      900

aacggcugcg gauuguucgg caagggcucc cuaguaacau gcgcaaaauu cgccugcucc      960

aagaaaauga ccggcaaauc gauccaaccc gaaaaccucg aauaccgcau aaugcuaucc     1020

guccacggcu cacaacacuc aggcaugaua guaaacgaca ccggacacga aacagacgaa     1080

aauagagcga aaguggagau cacucccaac agcccaagag cggaagccac acuaggaggc     1140

uucggcuccc uaggacucga cugugaacca cgaaccggac ucgauuucuc ggaccucuac     1200

uaccuaacaa ugaacaacaa acacuggcuc gugcacaaag aaugguucca cgacauaccc     1260

cucccuuggc acgcaggagc agacaccggc accccacacu ggaacaacaa agaagcccuc     1320

guagaguuca aagacgccca cgccaaaaga caaacaguug uaguacuggg cagccaagaa     1380

ggagccgugc acaccgcccu agccggagcc cuagaagccg aaauggacgg ggcuaaaggc     1440

cguuuaucca gcggccaccu aaaaugcaga cuaaaaaugg acaaacuaag guuaaaaggc     1500

guaagcuacu cccuaugcac ggcggcauuc accuucacaa aaauccccgc agaaacccuc     1560

cacggaacag ucacagugga aguucaauac gccggcacag acggacccug uaagguacca     1620

gcacaaaugg cugucgacau gcagacucug acaccaguug ggcgacucau caccgcaaac     1680

cccgucauca cugaaagcac cgaaaacucc aagaugaugc uagaacuuga cccaccauuc     1740

ggagacagcu acauaguaau uggcgucggc gaaaagaaaa ucacacacca cuggcacaga     1800

agcggcagca cgauagguaa agcauucgaa gcaaccguaa ggggcgccaa acgaauggca     1860

gugcucggag acaccgccug ggacuucgga agcguaggag gcgcccugaa cucacuaggc     1920

cgaggcauac aucaaauauu cggggcagca uucaaaagcc ucuucggcgg caugagcugg     1980

uucagucaaa uacuaauagg gacacugcua auguggcuag gacugaacac caaaaacggc     2040

ucaauaucac uaaugugccu agcacucgga ggcgugcuaa uauuccuaag cacagcaguc     2100

ucggcagacg uaggaugcag cguagacuuc uccaaa                               2136


<210>  2854
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long CDS opt

<400>  2854
cgcaucauca acgcaaggaa ggagaaaaag cgacgaggcg cagacgcgag cgucggcaua       60

guaggccucc ugcucacaac cgcgauggcc gccgagguca cacgcagagg cucggccuau      120

uacauguacc ucgacagaaa ugacgcaggc gaagccaucu cauucccgac cacacucgga      180

augaacaaau guuacauaca aaucauggac cuagggcaca ugugcgacgc aacuaugucc      240

uacgaaugcc cgaugcucga cgaaggagua gaaccagacg acguagauug cuggugcaac      300

acaacaagca cauggguagu cuauggcacg ugccaccaca aaaaggggga ggcgaggagg      360

agcagaaggg caguuacacu cccaagccac agcacgcgca aacuacaaac aaggagccag      420

accuggcuug aaagcagaga auacacaaag caccugauca gggucgaaaa cuggauauuc      480

cgcaacccgg guuucgcacu agcagcagca gcaaucgcau ggcuccuagg aagcagcacg      540

ucccaaaaag uaauauaccu cguaaugaua cugcucaucg ccccagcuua uucaaucagg      600

ugcaucggcg ugagcaacag agacuuugug gaaggaaugu caggcggcac gugggucgac      660

gucguucuag aacacggagg augcgucaca guaauggcuc aggacaaacc aacagucgac      720

auagagcuag uaacaacaac cguaagcaac auggcagaag uucgcaguua cugcuaugaa      780

gccucgaucu cagacauggc cucagacucg agaugcccca cacaaggaga agcguaccua      840

gacaaacaau cagacaccca auacguaugc aaaaggaccc uaguagaccg cggauggggg      900

aacgggugcg gacuauucgg aaagggaucg cucguaaccu gcgcaaaauu cgccugcagc      960

aaaaagauga cuggcaaaag cauacaaccc gaaaaccugg aauacagaau caugcuauca     1020

guccacggga gccaacacuc aggcaugaua guaaacgaca cgggccacga aacagacgaa     1080

aacagagcua aaguagagau aacacccaac agcccacgag cggaagcgac acuaggaggu     1140

uucggaagcc uaggccugga cugcgaaccg cgaacaggcc ucgauuucag cgaucucuac     1200

uaccugacca ugaauaacaa acacuggcuc guacauaaag agugguucca cgacauaccg     1260

cuaccauggc augccggagc agacaccgga acaccccacu ggaacaacaa agaggcccua     1320

gucgaguuca aagacgcgca cgccaaaaga caaacagugg ugguacuggg aucgcaggag     1380

ggcgcggucc acacugcacu cgccggcgcg cuagaagccg agauggacgg agcaaaagga     1440

cgccuaagca gcggacaccu caagugccga cuaaagaugg acaagcuccg ccuaaaaggg     1500

gucucguaca gucucugcac ggcagcauuc accuucacga agaucccggc ggaaacacug     1560

cacggaaccg uaacaguaga agugcaauac gccgggaccg acggcccgug caaagucccg     1620

gcccaaaugg caguagacau gcaaacgcua acaccaguag gaagacugau cacugccaac     1680

ccaguaauca cagaaucgac agagaacuca aaaaugaugc uagaauugga cccgcccuuu     1740

ggcgacagcu acaucgugau aggaguaggg gaaaagaaaa uaacacacca cuggcaccga     1800

agcggaagca caaucgggaa agccuucgag gcaacagucc gaggcgcaaa gagaauggca     1860

guccuuggag acacggcaug ggacuucgga agcgucgggg gcgccuugaa cucacuaggc     1920

aaagggaucc accaaauauu cggagccgcg uucaagagcc ucuucggcgg cauguccugg     1980

uuuucacaaa uacucaucgg aacauuacug auguggcucg ggcuuaacac caagaacggc     2040

ucaauaagcc ugaugugccu cgcccuaggg ggcguacuca uauuccuguc cacggccguc     2100

agcgccgacg uaggaugcag cguagauuuc agcaaa                               2136


<210>  2855
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long CDS opt

<400>  2855
cgaaucauca augcacggaa agagaaaaag cgccgagggg ccgacacgag cgucgggauc       60

guaggccuac ugcugaccac cgcaauggcc gcagaaguca cacgacgagg cagcgcauac      120

uacauguacc ucgacagaaa cgacgccgga gaagccaucu cauuccccac aacacucggg      180

augaacaaau gcuacaucca aauaauggac cuaggacaca ugugcgaugc aaccaugagc      240

uacgaaugcc caaugcucga ugaaggcguu gaaccagacg acguggacug cuggugcaac      300

acaacuagca ccugggucgu cuacggcaca ugccaccaca agaaagggga ggcacgcaga      360

agucgacggg ccguaaccuu acccagccac uccacaagaa aacuacaaac acgcucacaa      420

accuggcugg aaagucgaga auacaccaaa caccucaucc gaguggaaaa cuggaucuuc      480

agaaacccgg gacuagcacu agccgccgca gcaaucgcau ggcuacuagg aagcuccaca      540

agccaaaaag ucauauaccu cgucaugauc cuacugaucg ccccagcaua caguauacgc      600

ugcauaggag ugagcaacag agacuucgua gaaggcaugu caggaggaac gugggucgac      660

guaguccucg agcacggcgg cugcguaacc guaauggcac aagacaaacc caccguagac      720

auagaacucg ucaccacaac cguuagcaac auggcagagg ucaggucuua cugcuacgaa      780

gcaagcauaa gcgacauggc auccgacuca agaugcccca cccaaggcga agcauaccua      840

gacaaacaau ccgacacaca auacguaugc aaacgaaccc uaguagaccg gggcuggggc      900

aacggcugcg gacuauucgg caaagggagc cuagucacau gcgcaaaauu cgcauguucc      960

aagaaaauga ccggcaaauc cauccaacca gagaaccucg aguaccgcau aaugcuguca     1020

guacacggcu cccaacacuc cggaaugaua gugaacgaca ccggccacga aaccgacgag     1080

aacagagcca aagucgaaau aacacccaac ucaccccggg cagaggcaac ccucggaggc     1140

uucggcaguc ucggacucga cugcgaaccc cguaccgguc ucgacuuuuc agaccuauac     1200

uaccuuacua ugaacaacaa acacuggcua gugcacaaag agugguucca cgauaucccc     1260

cuaccauggc acgccggggc cgacaccggc acaccacacu ggaacaacaa agaagcgcuc     1320

gucgaguuca aagacgcgca cgcaaagcgc caaaccgugg uaguccuagg cagccaagaa     1380

ggggcagucc acacagcacu agccggagcg cuggaagcag aaauggacgg agccaaaggc     1440

cgccuaucca gcggacaucu aaaaugcaga cuaaaaaugg acaaacuccg ucucaaagga     1500

guauccuaca gccucugcac agcagcuuuc accuucacga agauccccgc cgaaacccuc     1560

cacggcacag ucacagucga aguccaguau gccggaacag acggaccaug caaagugccg     1620

gcccaaaugg ccguggacau gcaaacacuu accccaguag gccggcuaau cacagcaaac     1680

ccagugauca ccgaaagcac cgaaaacucc aaaaugaugu uagaacugga cccacccuuc     1740

ggcgauuccu acaucgucau cggcgugggc gaaaagaaaa ucacacacca uuggcaccga     1800

agcggcucca ccaucggaaa ggcauucgaa gccaccguaa gaggcgccaa gcgcauggca     1860

guacuaggag acacagcgug ggacuucggc agcgucggag gagcucuaaa cagccuggga     1920

aaggguauac accaaaucuu cggagcggcc uucaaaagcc ucuucggggg caugucaugg     1980

uucagccaga uccuaaucgg aacccuacua auguggcucg gccuaaacac caagaacgga     2040

agcauaucgc ugaugugcuu agcacucggc ggaguacuua ucuuccucuc aacagccgua     2100

uccgccgacg ucggcugcag cgucgacuuc agcaaa                               2136


<210>  2856
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long CDS opt

<400>  2856
cggaucauca acgcacgcaa agagaagaaa cgacggggag ccgacaccuc aguaggcauc       60

gugggacuac uucucacaac ggcaauggca gccgaaguaa cacgcagagg aagcgccuac      120

uacauguacc uagaccgcaa cgacgccggc gaagcaauca gcuuccccac aacccuagga      180

augaacaaau gcuacauaca aauaauggac cucggccaca ugugcgacgc cacaauguca      240

uacgaaugcc ccaugcuaga cgaaggagua gaacccgacg acgucgacug cuggugcaac      300

acgacaagca ccuggguugu auacggaacc ugccaccaca agaaaggcga agcacgccgc      360

ucacggagag ccguaacccu ccccucacac agcacaagaa aacugcaaac acggucgcaa      420

accuggcuag aaucacgcga auacaccaaa cacuuaaucc gagucgaaaa cuggauauuc      480

cgcaauccag gcuucgcacu cgccgcagcc gccaucgcau ggcuacuggg aagcuccaca      540

agccaaaaag uuaucuaccu cguaaugauc cuacuaaucg cccccgcuua cucgauacgg      600

ugcauaggag uaucgaacag ggacuucgua gagggcaugu ccggcggcac augggucgac      660

gucgucuuag agcacggggg cugcguaacc gucauggcuc aagacaaacc gaccguggac      720

aucgaacucg uaaccacaac cgugagcaac auggccgaag uaagaagcua uuguuacgaa      780

gcaagcaucu cagacauggc aagcgacagc agaugcccaa cccagggaga agcauaccua      840

gacaaacaau cagauacaca auacgugugc aaaaggacac ucguagacag aggauggggg      900

aacggaugcg ggcuuuucgg aaaaggauca cucgucacau gcgcaaaauu cgcaugcagc      960

aagaaaauga caggcaaauc aauccaaccc gagaaccugg aauacagaau aaugcuaucc     1020

guccacggga gccaacacuc cggaaugaua guaaacgaca caggacacga aacugacgag     1080

aaccgagcca aagucgagau cacaccaaac agcccacgcg cggaagccac acucggcgga     1140

uucggauccc ucggccucga cugcgagcca cgaaccggcc uagacuucuc ggaccuauac     1200

uaccuaacca ugaacaacaa acacuggcug gugcacaaag aaugguucca ugacauaccg     1260

cuuccauggc acgcaggagc agacaccggc acaccccacu ggaacaacaa agaagcgcuu     1320

guugaguuca aagacgccca cgccaaaaga caaaccguag uaguacucgg cagccaagaa     1380

ggugccguac acacagcgcu ugccggcgcc cucgaagcag aaauggacgg cgcaaaagga     1440

cgccuuagca gcggccaccu aaaauguagg cucaaaaugg acaaacuaag acuaaaaggg     1500

gucuccuauu cccuaugcac ggcagcauuc accuucacga aaauccccgc agaaacccuc     1560

cacggaaccg uaacagucga aguacaguac gcaggaaccg acggaccgug caaaguccca     1620

gcccagaugg caguggacau gcaaacccug acgccagucg gccgacucau caccgccaac     1680

ccugugauca cggagagcac agaaaacagc aaaaugaugc ucgaacucga cccacccuuc     1740

ggcgacucgu acauagucau aggcguaggc gaaaagaaaa uaacacacca cugguaccgc     1800

uccggcagca caaucgggaa agcauucgaa gccacaguca gaggggcgaa acgcauggcc     1860

guacuaggag acacagccug ggacuucggc agcguaggag gcgcacuaaa cagccuaggg     1920

aaaggcauac accaaaucuu cggagccgcc uucaaaagcc uauucggugg aauguccugg     1980

uucucccaaa uacuaauagg cacacuccua auguggcucg gacuaaacac caaaaacgga     2040

ucaaucuccc uaaugugucu cgcccuaggc ggaguacuca uauuucuauc cacagcaguc     2100

agcgcagacg uaggguguuc agucgacuuu agcaaa                               2136


<210>  2857
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long CDS opt

<400>  2857
cgaaucauaa acgcccggaa agaaaagaaa cgccgcggag ccgacaccuc cgucggaaua       60

guaggccugc ugcucaccac ggccauggcu gccgaaguga cccgccgagg aagugccuac      120

uacauguacc ucgacagaaa cgacgcaggc gaagcaauca gcuuccccac aacguuaggc      180

augaacaaau gcuauauaca gauaauggac cugggccaua ugugugaugc aaccauguca      240

uacgaaugcc ccaugcucga cgaaggaguc gaaccagacg acguagauug cuggugcaac      300

acaaccucga cauggguagu cuauggaaca ugccaccaca agaaaggcga agcccgccgc      360

ucaagacgag cgguaacgcu accuucgcau uccacacgaa aacuacaaac ccgcucccaa      420

acauggcuag aaagccgaga auacacaaaa caccucauca ggguagagaa cuggaucuuc      480

cgcaacccag gcuucgcacu cgccgcagca gccaucgccu ggcuacuagg aagcagcaca      540

ucgcaaaaag uuaucuaccu cguaaugauc cuccuuaucg ccccggccua cagcauacgc      600

ugcaucgggg ucuccaacag agacuucguc gaaggcauga gugggggcac guggguagac      660

gucguacuag aacacggcgg augcgugacc gucauggcac aagacaaacc caccguggac      720

auagagcuag uaacaaccac uguaucgaac auggccgaga ucagaagcua cugcuacgaa      780

gccuccauau ccgacauggc cagcgacagc agaugcccga cacaaggaga agccuaccuc      840

gacaaacaaa gcgacacaca auaugucugc aaacgcaccc uagucgacag aggcuggggc      900

aacgggugcg gccucuucgg caaaggaagc cucgucacau gcgcgaaauu cgcaugcucc      960

aaaaagauga ccggaaaauc gauacaaccc gagaaccucg aauaccgaau caugcuaagu     1020

guccacggca gccaacacuc aggaaugauc gucaaugaca ccggacacga gacagacgaa     1080

aaccgagcca aaguagaaau aaccccaaac ucaccacggg cagaggcgac gcuaggcgga     1140

uucggcuccc ugggccucga cugcgaacca cggaccggac ucgacuucag cgaccuauac     1200

uaccucacga ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauccca     1260

cugccauggc acgcaggcgc agauacaggc acaccccacu ggaacaacaa agaggcccua     1320

guagaguuca aagacgcaca cgccaaaaga caaaccguag ugguacucgg cagccaagaa     1380

ggcgcaguac acacagcacu cgccggcgca cuggaagcag aaauggacgg agccaaaggc     1440

cggcuaucca gcggccaccu gaaaugccgc cucaaaaugg acaagcucag gcucaaggga     1500

guaagcuaca gccuaugcac cgcagcauuu accuucacaa aaauccccgc ggaaacucug     1560

cacggcaccg ucacagucga gguucaauac gcaggcaccg acggcccaug caaggucccc     1620

gcacaaaugg cuguggacau gcaaacacuc accccugucg gcagacucau aaccgccaac     1680

ccagucauaa ccgagagcac agaaaauucc aaaaugaugc uagaacuaga cccucccuuc     1740

ggggacagcu acaucgucau cggcguaggg gagaaaaaga ucacccacca cuggcaccgg     1800

ucaggcucaa ccauaggcaa agccuucgaa gcaacaguaa gaggagccaa acgcauggcc     1860

guacugggag auacagccug ggauuucgga ucugucggag gcgcacuuaa cagccuuggc     1920

aaaggcaucc accaaaucuu cggcgccgcc uucaaguccc ucuucggcgg aaugagcugg     1980

uucagccaaa uccuaaucgg cacccuccua auguggcucg gacucaacac caaaaacggu     2040

uccaucucau uaaugugccu agcccucgga gguguccuca uauuccuauc caccgcaguc     2100

uccgccgacg uaggcuguag cgucgacuuc ucuaag                               2136


<210>  2858
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long CDS opt

<400>  2858
cgcaucauca acgcaagaaa agaaaagaaa cgcaggggag cagacaccuc ggucggcaua       60

gucggacucc uacucaccac cgccauggcu gcugaaguua cacgcagagg aucggccuac      120

uacauguacc uggaccgcaa cgacgcaggg gaggccauau cguuccccac cacacuugga      180

augaacaaau gcuacauaca aaucauggac cucgggcaca ugugcgacgc aaccaugucc      240

uacgaaugcc ccaugcucga cgaaggagua gaaccagacg augucgacug cuggugcaac      300

accacgagca caugggucgu uuacggcaca ugccaccaca agaaagguga agcccgccgc      360

agucgacgag ccgucacccu uccaagccac uccacacgaa aacuccaaac caggagccaa      420

accuggcucg aaagccgaga auacaccaaa caccucauaa gaguagagaa cuggauauuc      480

cgcaacccag gcuucgcccu cgcagccgcc gccaucgcgu ggcuccuagg caguagcacc      540

agccaaaaag ugauauaccu agucaugaua cuacugaucg caccagcaua cucaauccgg      600

ugcauaggag uaucuaaccg agacuucgua gaaggcaugu caggagggac auggguggac      660

guaguccucg aacacggagg gugcguaaca guaauggccc aagacaaacc caccgucgac      720

aucgagcuag ucaccacgac cgucagcaac auggcagaag uccgguccua cugcuacgaa      780

gcaagcaucu cggacauggc cagugacucc cgcugcccca cgcagggcga agcauaccuc      840

gacaaacaau ccgacaccca auacgucugu aaacgcacac ucguagaccg cggcugggga      900

aacggcugcg ggcuauucgg aaagggcagc uuagucacau gcgccaaguu ugcaugcuca      960

aagaaaauga ccggcaaaag cauacaaccc gaaaaccucg aauaccguau caugcugucc     1020

guacacggau cacaacacag cggaaugauc gucaacgaca ccggccacga aacagacgaa     1080

aacagagcaa aagucgaaau aacucccaau agcccacgcg cagaggccac acugggcggu     1140

uucggcagcc ugggccuaga cugcgaaccc cgcacaggcc ucgacuuuuc cgaccucuac     1200

uaccucacca ugaacaacaa acacuggcuc guccacaaag aaugguucca ugacaucccc     1260

cucccauggc acgccggcgc cgacaccgga accccacacu ggaacaacaa agaagcccua     1320

gucgaguuca aagacgccca cgcgaaacga caaaccguag uaguacucgg cagccaagaa     1380

ggcgcaguuc auacggcccu cgcgggagca cucgaagcug aaauggacgg cgcaaaagga     1440

cgacucucau caggacaucu gaaaugccga cucaaaaugg acaaauuaag acucaaaggc     1500

guauccuacu cgcucugcac ggcagcauuc acguuuacaa aaauaccagc agaaacccug     1560

cacggcaccg uuaccguaga aguccaauac gccgguaccg acggcccgug caagguccua     1620

gcacaaaugg cagucgacau gcaaacauug acacccgucg gccgacugau cacagcaaac     1680

cccgugauca ccgaaucaac cgaaaacucc aaaaugaugc ucgaacugga cccaccauuc     1740

ggcgauagcu auaucgucau cggagucggc gaaaagaaaa ucacccacca cuggcaccga     1800

uccggcucca caaucggaaa agcauucgaa gcaacaguac ggggcgcaaa acggauggca     1860

guccucggcg acacagccug ggacuucggc agcgucggcg gcgcccucaa cagccuaggc     1920

aaaggcaucc accaaauauu cggugcagcc uucaagagcc ucuucggagg aaugagcugg     1980

uucagccaaa uacucaucgg cacacuccua auguggcugg gccucaacac caaaaacggc     2040

agcauaagcc ucaugugucu ggcccuagga ggaguacuaa uauuccuauc cacugccguc     2100

agcgcagacg uuggcugcag cguagacuuc agcaaa                               2136


<210>  2859
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long CDS opt

<400>  2859
cgcaucauca acgcacgaaa ggagaaaaaa cgccgagggg ccgauaccuc cguuggcauc       60

gugggacucc uccuaacuac agcuauggcc gcggaaguga cccgccgcgg aagcgcuuac      120

uacauguacc uagaucgcaa cgacgccggc gaagccaucu cauucccaac gacacugggc      180

augaacaaau gcuacaucca aaucauggac cuaggccaca ugugcgacgc caccaugucu      240

uacgaauguc ccacacuuga cgaaggcgua gagcccgacg acguagacug cuggugcaac      300

acgacgucaa ccuggguugu auacggcaca ugccaccaca aaaagggaga agccagacgc      360

agcagaagag ccgucacacu acccagccac ucgaccagaa aacuacagac ccgcagccaa      420

acauggcugg aaucccgcga auacacaaaa caccuaauac gcguagaaaa cuggauauuc      480

aggaacccgg gcuucgcccu agccgcagca gcaaucgccu ggcuccuagg cuccuccaca      540

ucccaaaaag ucauauaucu cgucaugauc uuacucaucg ccccagccua cucuauacgc      600

ugcaucggcg ucuccaaccg cgacuucguc gaaggcaugu ccggaggaac auggguagac      660

guaguacugg agcacggggg augcgucaca guaauggccc aagacaaacc caccguagac      720

auagaacuag ucaccaccac agucucaaac auggccgaag uccgcucaua cuguuacgaa      780

gcaagcauau ccgacauggc cuccgacucg cggugcccaa cccaaggcga ggccuaccua      840

gacaaacaaa gcgacaccca auacgucugc aagcguacgc ugguggaccg uggaugggga      900

aacggaugcg gacuauucgg caaaggcagc cucguuaccu gcgcaaaauu cgccugcucc      960

aaaaaaauga caggcaaauc gauucaaccc gagaaccucg aauaccgaau aaugcuaagc     1020

guccacggau cacagcacuc cggcaugauc gucaacgaca ccggacacga gacagacgag     1080

aaccgcgcaa aaguggaaau caccccgaac uccccccgug cagaggcaac ccuaggcggc     1140

uucggcucac ucggacucga cugugaaccc agaaccggcc ucgacuucuc cgacuuguac     1200

uaccucacaa ugaacaacaa acacuggcua guccacaaag aaugguucca cgacaucccc     1260

cuccccuggc acgcaggagc ggacaccgga accccacacu ggaacaacaa agaagcccuc     1320

gucgaguuca aagaugccca cgcaaaacgc cagacagucg ucguccuagg aagccaagaa     1380

ggagcaguac acacagcacu cgcaggagcc cuagaagccg aaauggacgg ugcaaaaggc     1440

agacucagca gcggacaucu caaaugcaga cuuaaaaugg acaaacuccg gcucaaaggc     1500

gucagcuaca gccucugcac agcagccuuc acauucacaa aaauccccgc ggaaacccuc     1560

cacggcacug uaacggucga aguccaauac gcaggaacgg auggcccaug caaaguccca     1620

gcccaaaugg cagucgacau gcaaacccuc acccccgucg gccgucucau aaccgcaaac     1680

ccggucauca cagaaagcac cgaaaacagc aaaaugaugc uggaacuaga cccacccuuc     1740

ggagauuccu acaucgucau cggcguaggc gaaaaaaaaa uaacccacca cuggcacaga     1800

ucagguucga caaucggaaa agcguucgaa gccaccguaa gaggcgccaa acgcauggcc     1860

guccucggag acaccgcaug ggacuucgga ucuguaggug gagcccuuaa cucccuaggc     1920

aaagguaucc accaaaucuu uggagcugca uucaaaucac uauucggcgg aaugucaugg     1980

uucagucaaa uacucaucgg gacccuacuc auguggcuag gccuaaauac caaaaacggg     2040

ucgauaucac uaaugugccu cgcacucggc ggcguacuca uauuucucuc aaccgcggua     2100

agcgcagacg ugggaugcuc agucgacuuc agcaaa                               2136


<210>  2860
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long CDS opt

<400>  2860
cgaauaauaa augcacgaaa ggaaaagaaa cgaagaggag cagacacauc cguaggaaua       60

gugggacuac uacuaaccac ggccauggca gcagaaguaa ccaggagggg aucggccuac      120

uacauguacc ucgacagaaa cgacgcagga gaagcaaucu ccuuccccac aacacucgga      180

augaacaaau gcuacaucca gauaauggau cuuggacaca ugugcgacgc cacaaugagc      240

uacgagugcc ccauguuaga cgaaggcguu gaaccagacg acgucgauug cuggugcaac      300

acaaccucaa cuuggguagu cuacgggaca ugccaucaca agaaagggga ggcccgcagg      360

uccagacgag ccguaacacu ccccagccau ucaacacgca aacuccagac gcgcagccag      420

accuggcucg aaagccgaga auacacaaaa caccuaaucc gcguagaaaa cuggauuuuu      480

agaaaccccg gauucgcacu cgcagcagcg gcuauagccu ggcuccucgg auccagcacc      540

agccaaaaag uaaucuaccu cguaaugauc cugcucaucg ccccagcaua cagcauccga      600

ugcaucggag ugagcaacag agacuucgua gaaggcaugu caggcggcac cuggguagau      660

guaguacucg aacacggagg augcguaacg guaauggccc aagacaaacc aacaguggac      720

aucgaacucg ugaccaccac cgucuccaac auggcagaag uacggucaua cugcuacgaa      780

gccucaauca gcgacauggc aagcgacucc agguguccga cccaaggcga agcauaccuc      840

gacaaacaaa gcgacaccca auacgucugc aaacggaccc uaguagaccg aggcuggggu      900

aacggcugcg gccucuuugg aaaaggaagc cucguaacau gcgccaaauu ugccugcagc      960

aaaaagauga cagggaaaag cauacaacca gaaaaccuag aauacagaau aaugcucucc     1020

guccacggca gccaacacuc aggcaugaua guaaacgaca ccggacacga aacggacgag     1080

aacagagcca aagucgaaau aaccccaaac agcccacgcg ccgaagccac gcucgggggc     1140

uucggaucau ugggauuaga cugugaaccc cgcacagguc ucgauuucag cgaccucuau     1200

uaccucacca ugaacaacaa gcacuggcug guacacaaag aaugguucca cgacauacca     1260

cuccccuggc acgccggugc agacaccggc acaccccacu ggaauaauaa agaagcguug     1320

gucgaguuca aagaugccca cgcgaagaga caaaccguag uagugcucgg cagccaagaa     1380

ggagccguac acaccgcccu cgccggcgcu cucgaagccg aaauggacgg agccaaaggu     1440

cgccugagcu ccggccaccu aaaaugccga cuaaaaaugg acaaacuuag acuaaaagga     1500

guaucauacu cacucugcac ggcagccuuu accuucacca aaaucccggc agaaaccuua     1560

cacggaacag uaacagucga agugcaauac gcaggcacag acggccccug caaagucccc     1620

gcacagaugg ccguagacau gcagacccuc accccggucg gacgccucau cacagcuaac     1680

ccggugauca ccgaauccac cgaaaacagc aaaaugaugc ucgaacuuga cccacccuuc     1740

ggcgacagcu acaucgucau cggaguggga gaaaagaaaa ucacgcauca cuggcaccgu     1800

agcggaucaa cgauaggaaa ggccuucgag gccacgguaa gaggcgcgaa aagaauggca     1860

guccuaggag acacagccug ggauuucggg ucggucggug gcgcacucaa cucacuaggc     1920

aaaggcaucc aucaaaucuu cggcgccgcc uucaaaagcc uauucggagg aaugagcugg     1980

uuuagccaaa uacuuauagg cacacuccua auguggcucg gacucaauac aaagaacgga     2040

agcaucucac uaaugugucu agcccuaggc gggguccuca uauuccuauc cacagucguc     2100

agcgccgacg ucggcugcag cguugacuuc agcaaa                               2136


<210>  2861
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long CDS opt

<400>  2861
agaaucauca augccagaaa agaaaagaaa cggcgcggag ccgacacauc cgucgggaua       60

guaggacucc ugcucacaac cgcaauggca gcggagguca cucgacgggg cucagcguac      120

uacauguacc ucgacagaaa cgacgcagga gaagcaauau cauuccccac aacacuagga      180

augaacaaau gcuauaucca gauaauggac cuaggccaca ugugcgacgc gacaaugagc      240

uacgaguguc caaugcucga cgaaggagua gaacccgacg acguagacug cuggugcaac      300

accaccuccg cauggguagu guacggaaca ugccaccaca agaaaggcga agccagacgc      360

ucgcgcagag ccgucacccu acccagccac uccacccgaa aacuucaaac ccgcccacaa      420

acauggcuag aaucacggga auacaccaaa caccuaauac gcguugaaaa uuggauauuc      480

agaaaccccg gauucgcacu agcugccgcu gccauagccu ggcuacuagg gaguucaaca      540

ucacaaaaag ucaucuaccu agucaugauc uuacucaucg cacccgccua cagcauccgc      600

uguauaggag ucagcaaucg agacuuugua gagggaauga gcgggggcac cuggguagac      660

gucguccuag aacacggcgg augcguaacc gucauggcgc aagacaaacc aaccgucgac      720

aucgaacucg ucacaacaac cgucucaaac auggccgaag uucgaucaua cugcuacgaa      780

gcgaguauau cagacauggc cagcgacucc cggugcccca cccaagguga agcauaccug      840

gacaaacaaa gcgacaccca guacgucugc aaaaggaccc ugguggaccg cggauggggc      900

aacggcugcg gacucuucgg caaaggcagc cucguuacgu gcgcuaaauu cgccugcagc      960

aagaaaauga caggcaaauc cauccaaccc gaaaaccuag aauaccgaau caugcuaagc     1020

guacacggcu cgcaacacuc aggcaugaua gucaacgaca ccgggcacga aaccgacgaa     1080

aaccgagcca aaguagaaau cacgccaaac ucaccccgag ccgaggccac acucggcggc     1140

uucggauccc ucggccuaga cugcgaaccc agaacagguc ucgacuucuc cgaccuauac     1200

uaccugacaa ugaauaauaa acacuggcuc guccacaaag agugguucca cgauauccca     1260

cugcccuggc augccggcgc ggacaccggc acaccccacu ggaacaacaa agaagcccua     1320

guagaguuca aagacgcaca cgccaaaagg caaaccgucg ucguccuagg aucccaagaa     1380

ggcgccgucc acaccgcccu cgccggcgcc cuagaagcag aaauggacgg agccaaaggc     1440

cgccucucau caggccaccu caagugccgc cuaaaaaugg acaagcuacg acuaaaaggc     1500

gugagcuacu cccucugcac agcggccuuc accuucacga agauaccagc cgaaacacuc     1560

cacgggaccg ucaccguaga gguacaauac gcaggaacag acgggcccug caaaguaccc     1620

gcccaaaugg ccguagacau gcaaacccuc accccaguag gacgacucau aaccgccaac     1680

ccagucauaa ccgagagcac cgaaaacagc aaaaugaugc uagaacuaga cccacccuuc     1740

ggcgauaguu acauuguaau aggcguaggc gaaaagaaaa ucacacacca uuggcacaga     1800

uccggaucaa ccauuggcaa agcauucgaa gcaaccguac gcggcgcuaa acgcauggcc     1860

guacucggcg auaccgcaug ggacuuugga uccgucgggg gagcauuaaa cucacuagga     1920

aaaggcaucc accagauauu cggcgccgca uucaaaucac ucuucggagg aaugucaugg     1980

uuuagccaaa uacucauagg cacacuccuc auguggcuag gccucaacac caaaaacgga     2040

agcauauccc uuaugugccu cgcccucggc gguguucuca ucuuucuauc cacagccguc     2100

agcgccgacg ucggcugcuc agucgacuuc ucaaaa                               2136


<210>  2862
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long CDS opt

<400>  2862
cgcaucauca acgcccgcaa agaaaagaaa cgacgcggcg cugacaccuc cguaggcaua       60

guaggacucc uacucaccac cgcaauggca gcagaaguca cccgccgagg cagcgcauac      120

uacauguacc ucgacagaaa cgacgccggu gaagccauau cauuccccac cacccucgga      180

augaacaagu gcuacaucca aauaauggac cuuggccaca ugugcgacgc caccaugagc      240

uacgaaugcc caaugcucga cgaaggagua gagccagacg acgucgacug cuggugcaac      300

acgaccucca cuugggucgu cuauggcacg ugccaccaca agaaaggaga agccagaaga      360

ucacgccgug caguaacgcu accgagccac ucaacccgaa aacuucaaac ccguagccag      420

accuggcuag agucacgcga auacacuaaa caccugaucc gcguagaaaa cuggauauuu      480

cgcaaccccg gcuucgcgcu cgcagccgcg gcaauagccu ggcugcuagg aucguccacc      540

uccaaaaagg uuauauaccu cguaaugauc cuccucauag cucccgcaua cucaauaagg      600

ugcauaggcg ucagcaaccg cgacuucguc gaaggcaugu ccggaggcac cuggguagac      660

gugguccucg aacacggagg augcgucaca gugauggcac aagacaaacc caccgucgac      720

auagaacucg ucaccacuac agucagcaac auggccgaag ucagaucaua cugcuacgaa      780

gcaagcauca gcgacauggc auccgacucg cgcugcccca cccaaggcga agcauaccua      840

gacaaacaaa gcgacaccca guacguaugc aaacgcacac ucgucgaccg gggcuggggg      900

aacggcugcg gucucuucgg aaaaggcucc cucguaaccu gcgccaaauu cgcaugcucc      960

aagaaaauga ccggcaaaag cauccaaccc gaaaaccucg aauaccgaau caugcuaucg     1020

guccacgguu cccaacacag cggaaugaua gucaacgaca ccggacacga aaccgacgaa     1080

aaccgagcca aagucgaaau aacacccaac agcccgcgag cagaagccac acuaggcgga     1140

uucggaagcc ucggccuaga cugcgagccc cgcacaggau uagacuuuuc cgacuuauac     1200

uaccuaacga ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauacca     1260

cuaccauggc acgcaggggc cgacaccgga acaccacacu ggaacaauaa agaggcacuc     1320

guagaauuua aagacgcaca cgcaaaacga cagaccguug ucguacucgg aucccaggag     1380

ggagccgucc acaccgcacu agccggugcc cuagaagcag aaauggacgg agcaaaagga     1440

agacuaagua gugggcaccu caaaugccgu cuaaaaaugg acaagcucag gcuaaaaggc     1500

gugagcuacu cccucuguac agccgccuuc accuucacca aaauccccgc ugaaacacua     1560

cacggaaccg ucaccgucga aguccaauac gccggcaccg acggccccug caaaguuccc     1620

gcccagaugg ccgucgacau gcaaacccuc acacccgucg gucgacucau cacagcgaac     1680

cccgugauaa ccgaaagcac ugaaaauagc aaaaugaugc ucgagcugga cccacccuuu     1740

ggggacagcu acauaguaau cggcgucggc gaaaagaaaa ucacccacca cuggcacaga     1800

agcgggagca ccauaggaaa agcauucgaa gcaacagucc gcggugcaaa acguauggcc     1860

guacucggag acacagccug ggacuucggc agcguaggcg gugcacuaaa cagccuagga     1920

aaaggaauac accaaauauu cggagccgcc uucaaaagcc ucuucggagg caugucgugg     1980

uucucgcaga uucucauagg cacacuucua auguggcuag gccuaaacac caagaacggc     2040

ucaaucagcc uaaugugccu ggcacucgga ggcguccuaa ucuuccuauc aacagcaguc     2100

agcgcagacg ucggaugcag cguagacuuu uccaaa                               2136


<210>  2863
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long CDS opt

<400>  2863
agaaucauca acgcacgaaa agaaaagaaa cgacgcggag ccgacacauc cguaggcauc       60

guuggacuac uauuaaccac agcaauggcc gccgaaguaa caagaagagg uagcgcauac      120

uacauguauc uugaccggaa cgacgccggc gaagccauca gcuucccgac cacacuaggg      180

augaacaaau gcuacauuca aauaauggac cugggccaua ugugcgacgc aacaauguca      240

uacgaaugcc cgaugcucga cgagggcgua gaaccggacg acgucgauug cuggugcaac      300

acaacuucaa cuugggucgu cuacgguacc ugccaccaca agaaaggcga agcccgccgc      360

ucgcggcgcg caguaacgcu acccagccac uccacgcgca aacuacaaac ccgcucccag      420

accuggcuag aaucgcgaga guacacaaaa caccuaaucc gcguggaaaa cuggauuuuc      480

cgaaacccag gcuucgcccu cgcagccgca gcgaucgcau ggcuccuagg caguagcaca      540

cgacaaaaag uaauauaucu uguaaugaua cuucucaucg cccccgccua cagcauccga      600

ugcauuggcg ucagcaacag ggacuucgua gaaggcaugu ccggaggaac cuggguagac      660

gucguucucg aacacggcgg cugcgucacc guaauggcac aagauaaacc gaccguagac      720

aucgaacuug ugaccaccac gguguccaac auggccgaag uaagaagcua cugcuaugaa      780

gcaagcauca gcgacauggc cagcgacagc agaugcccca cacaaggcga ggcuuaccuc      840

gauaaacaau cagacaccca guacguaugc aagcgaaccc uagucgacag aggcugggga      900

aacggcugcg gccucuucgg uaaaggguca cucguuaccu gcgccaaauu cgccugcagc      960

aagaaaauga ccggcaagag cauccaaccc gaaaaccucg aauaccgaau caugcuguca     1020

guccacggua gccaacacuc agguaugaua gucaacgaca ccggacacga aacagacgaa     1080

aacagagcaa aagucgaaau cacacccaac agcccuagag cggaagccac ccucggaggc     1140

uucggcagcc uaggccuaga cugugaacca cgcacaggcc ucgacuucag cgacuuauau     1200

uaucucacaa ugaacaacaa gcacuggcua guccacaaag aaugguucca cgacauacca     1260

cuacccuggc acgcaggagc agacaccggc acaccccacu ggaacaacaa agaggcacuc     1320

guugaauuua aagacgcaca cgccaaacga caaaccgucg uaguacuagg aagccaagaa     1380

ggcgcaguac acacagcccu agccggggcg cuagaagccg aaauggacgg cgccaaggga     1440

cgccucuccu ccggacaccu caaaugucgg cuaaaaaugg acaaacuaag acuaaaaggg     1500

guauccuaca gccucugcac ggcagccuuc accuuuacca aaaucccagc cgaaacccua     1560

cacgggacag ucacaguaga aguucaauac gccgggaccg acggaccaug caaggucccc     1620

gcgcaaaugg ccguggacau gcaaacccua acaccuguag gccgacucau aaccgccaac     1680

cccguaauaa ccgaaagcac cgaaaacucc aaaaugaugc ucgaauuaga ucccccauuc     1740

ggcgacagcu acauaguaau aggcguaggc gagaaaaaga ucacgcacca cuggcacaga     1800

uccggcagca ccaucggaaa agccuucgag gcaaccgugc gaggcgccaa acgcauggcc     1860

guccucggag acacggcaug ggacuucgga ucaguaggcg gagcccuaaa cagccuagga     1920

aaaggaauac accaaauauu cggcgcagcc uucaaauccu uauucggcgg caugagcugg     1980

uucucacaaa uacuaaucgg gacccugcuc auguggcucg gacucaacac caaaaacgga     2040

agcauauccc ucaugugccu cgcacucggg ggcguacuaa ucuuccugag cacagcagua     2100

uccgccgacg uagguugcuc ggucgacuuc uccaaa                               2136


<210>  2864
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long CDS opt

<400>  2864
cgcauaauca acgcgcgaaa agaaaagaag cgaagaucgg cagacaccag cgucggcaua       60

gucggccuac uccucaccac cgcgauggcc gcagaaguca cacgcagggg cuccgcauac      120

uacauguacc ucgaccgaaa cgacgccggc gaagccauca guuuccccac aacacuagga      180

augaacaaau gcuacauaca aaucauggac cuggggcaca ugugcgacgc cacaaugucc      240

uacgaaugcc ccaugcugga cgaaggcgug gaaccggacg acguagacug uuggugcaau      300

accaccagca ccuggguagu guauggcaca ugccaccaca agaaaggaga agccagacgc      360

ucacggcgcg cagugacacu cccaagccac agcacucgua aacuacaaac ccgaagccaa      420

accuggcuag aaucccgaga auauacaaaa caccucauaa gaguagaaaa cuggauauuc      480

cgaaaccccg gauucgcgcu cgcagccgca gcaauagcau ggcuacuugg cucaagcacc      540

agccaaaaag ucauauaccu agucaugaua cugcucaucg ccccagcaua cucaaucaga      600

ugcauaggcg uaagcaaccg agacuucguu gaagggaugu cuggagggac augggucgac      660

gucguacuag aacacggagg cugcgucacg gucauggccc aggacaaacc aacgguagau      720

auagagcucg uaacgacaac cguaagcaac auggcagaag uacgcaguua cugcuacgaa      780

gccagcauau cagauauggc cuccgacucc cgcugcccca cacagggaga ggccuaccuc      840

gacaaacaau cagacacaca auacgucugc aaacggacgc uaguagaccg cggcugggga      900

aauggaugcg gucucuucgg caaaggaucg cuagugacau gugccaaauu cgcaugcucc      960

aagaaaauga caggcaaauc cauccaacca gaaaaccuag aguacagaau aaugcucagc     1020

guacacggca gccaacacag cggcaugaua gucaaugaca caggacacga aaccgacgaa     1080

aaccgggcca agguagagau aacccccaac agcccaagag cagaggcgac ccugggcggu     1140

uucggcagcc ucggccuaga cugcgagcca agaacaggac ucgacuucag cgaccucuac     1200

uaccuaacaa ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauacca     1260

cuaccauggc acgccggcgc agacaccggg acaccacacu ggaacaacaa ggaagcccua     1320

gucgaguuca aggacgcaca cgcaaaacga cagaccgucg uaguacuagg aucacaagaa     1380

ggggccguac acacagcccu agccggugca cuagaagcag aaauggacgg agcaaaagga     1440

cgccuaucaa gcggccaccu aaaaugccgc cucaaaaugg auaaacuucg ucuaaaagga     1500

gucagcuaca gcuuaugcac ugcagcauuc acauucacaa aaaucccagc agaaacacua     1560

cacggaaccg ucacagugga aguccaauac gccggaacag acggcccaug caaaguacca     1620

gcccaaaugg cggucgauac ccagacacuc accccagucg ggcgacucau aacagcaaac     1680

ccagucauca ccgaaucgac agaaaacuca aaaaugaugc uagaacucga cccacccuuc     1740

ggcgacagcu auaucguaau agggguagga gagaaaaaga uaacacacca cuggcaccgg     1800

agcggcagca ccauaggcaa agcguucgaa gccacagucc guggagcaaa aagaauggca     1860

guauuggggg auacggccug ggacuucggc agcguaggag gagcccucaa cagccuagga     1920

aaagggaucc accaaauauu cggcgccgcg uucaaaagcc uguucggagg aaugagcugg     1980

uucucccaaa uacuaaucgg gacccuccuc auguggcucg gacuuaacac caaaaacgga     2040

agcaucucac uaaugugccu agcacuagga ggagugcuaa uuuuccugag uacagccgua     2100

uccgcggacg ucggcugcuc cguugacuuc uccaag                               2136


<210>  2865
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long CDS opt

<400>  2865
agaaucauca acgcaagaaa agaaaagaaa agacgaggag ccgacacaaa cguaggaauc       60

gucggacuac uccucacaac ugcaauggca gcagaaguca cgcgacgagg auccgccuac      120

uacauguacc uagaccgcaa cgacgcgggc gaagcaauau cauucccaac cacccugggg      180

augaacaaau gcuacauaca gauaauggac cucggacaca ugugcgacgc gaccaugagc      240

uacgagugcc caaugcucga cgaaggcgug gaaccagacg augucgacug cuggugcaac      300

accacgagca caugggucgu auacgggaca ugccaccaca agaaaggaga agcgaggcga      360

ucccggagag ccgucacccu cccgagccac ucaacacgaa aacuacaaac aagaucgcaa      420

accuggcuag aaucgagaga auauaccaaa caccuaauaa ggguagaaaa cuggaucuuc      480

cgcaacccgg gcuucgcgcu agccgcagcc gcaauagccu ggcugcuagg gagcucaaca      540

agccagaaag uaaucuaucu aguaaugauc cuccuaauag ccccagcaua cagcauccgc      600

ugcauaggcg uaagcaacag ggauuucgua gaaggaauga gcggaggcac auggguagac      660

guagugcucg agcauggagg augcgucaca gucauggcac aagacaaacc aacagucgac      720

auagaacugg uaacaaccac aguguccaac auggcagaag uccgcagcua cugcuacgaa      780

gccagcauaa gcgacauggc aagcgacagc cggugcccaa cacaaggaga agccuaccua      840

gacaaacaau ccgacacaca auacguuugc aaacgcacgu uaguagaccg cggaugggga      900

aacgguugcg gacucuucgg caaagguagc uuggugacau gcgccaaauu cgccugcagu      960

aagaaaauga caggcaaaag cauccaacca gagaaccuag aguacagaau caugcucagc     1020

guccacggcu cccaacacag uggaaugauc guaaacgaca caggacacga gacggacgaa     1080

aacagagcaa aaguagaaau cacacccaac agcccacggg cagaagccac cuugggagga     1140

uucggauccc uaggucucga cugcgaaccc agaaccggau uagauuucuc cgaccucuac     1200

uaccuaacca ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauccca     1260

uuaccauggc acgccggugc cgacacagga accccgcacu ggaacaacaa agaagcccua     1320

guggaguuca aagacgccca cgcaaaaaga caaacaguag ugguucuagg aucccaagaa     1380

ggugcagucc acacugcacu ggcgggugca cucgaagccg agauggacgg cgcaaaaggc     1440

cggcuaucga gcggccaccu aaaaugcagg cucaagaugg acaagcuaag acucaaaggc     1500

gugagcuacu cacucugcac cgcagcguuc acauucacca aaaucccagc cgaaacccua     1560

cacgggacag ucacagugga gguccaguau gcaggcaccg auggcccaug caaaguaccg     1620

gcccaaaugg caguagacau gcagacacua acaccggucg gccgacuaau aacagcaaac     1680

ccaguaauca cagaaagcac cgagaacucg aaaaugaugc uagaacucga ucccccauuc     1740

ggcgacagcu acaucguaau cgggguaggg gagaagaaaa ucacccacca uuggcaccgg     1800

agcggcagca cgaucgggaa agcauucgaa gccacgguac gaggagcgag acgaauggca     1860

guccucgggg acaccgcaug ggacuucggc aguguuggcg gagcauugaa cucgcucggg     1920

aaagggauac accagauuuu cggggcagca uucaaaucac uauucggagg gaugagcugg     1980

uucagccaga uccucaucgg aacccuacug auguggcuug gacuaaacac caaaaacgga     2040

uccauaagcc uaaugugccu agcccuggga ggaguccuaa uauuccuaag uacagccgua     2100

agugccgacg uaggaugcag cgucgacuuc ucaaaa                               2136


<210>  2866
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long CDS opt

<400>  2866
cgaaucauaa acgcccgaaa agaaaagaaa cgacgcggag cagaaaccuc gguaggcaua       60

guaggacugc uacuaaccac agcgauggca gccgaaguaa caagaagagg uucagccuac      120

uacauguacc ucgaccgcaa cgacgcaggc gaagcgauca gcuucccaac gacgcuagga      180

augaacaaau gcuacauaca aauaauggac cugggccaua ugugcgacgc cacgaugagu      240

uacgaaugcc ccauguugga cgagggaguc gagccagacg acgucgacug cugguguaac      300

acaacgagca cgugggucgu cuacggaacc ugccaccaca agaaaggcga agcccgacga      360

ucaagacgcg caguaacacu accgagccac uccacacgca aacuccaaac aagaagccaa      420

accuggcucg aaagccgaga auacacaaaa caccuaaucc gcguagaaaa cuggaucuuc      480

cgcaacccag gcuucgcacu agcagccgcc gcaauagcau ggcuccucgg uagcagcacc      540

agccaaaaag ucaucuaucu aguaaugaua cuccuuauag cgccagcaua cuccauucga      600

ugcaucggcg uuaguaaccg cgacuucgua gaaggaaugu cggguggaac cuggguagac      660

gugguacucg agcacggugg cugcguaacg gugauggcgc aagacaaacc cacagucgac      720

aucgaacucg ugacaacaac aguaagcaac auggccgaag uccggucaua cugcuacgag      780

gcaucaauca gcgauauggc cucagacagc cgcugcccca cgcagggcga agccuaccuc      840

gacaaacagu ccgacacaca auacgucugc aaacgaacgc uagucgacag gggauggggg      900

aacggcugcg gacucuucgg caaaggaucc cuaguaaccu gcgcaaaauu cgccugcuca      960

aagaaaauga caggaaaaag cauucaacca gaaaaucuag aauacagaau caugcuguca     1020

guucacggcu cacaacacag cggcaugaua guaaacgaca caggccacga aacagacgag     1080

aaccgagcca aaguagaaau cacucccaau agcccaagag cagaagcaac ccuuggaggg     1140

uucgggucac uuggacuaga cugcgaacca cgaaccggcc uagacuucag cgaccuuuac     1200

uaccucacca ugaacaauaa acacuggcuc guacauaaag aaugguucca cgacauccca     1260

cugccguggc acgcaggcgc cgacacaggu acaccacacu ggaacaacaa agaagcacug     1320

guagaguuca aagacgccca ugcgaaacgc cagaccgucg uugugcuggg cucacaagaa     1380

ggagccguac acacagcacu ggccggagcc uuagaagccg aaauggacgg cgcgaaagga     1440

cgcuuguccu ccggccaccu caaaugccgg cuaaaaaugg acaaacuacg ccugaaagga     1500

guuucguauu cacugugcac agcagcguuc accuucacca aaauaccagc cgaaacccua     1560

cacggcacag uaaccgucga aguacaauac gcgggaaccg acgggcccug caaaguucca     1620

gcacaaaugg cgguagacau gcaaaccuua acaccgguag gcagacuaau cacagccaac     1680

ccagucauca ccgaaaguac ggaaaauucg aaaaugaugc uagaacuaga cccgccauuc     1740

ggagacagcu acauaguaau cggggucggc gaaaagaaaa uaacacacca cuggcacaga     1800

agcggcagca cgauaggcaa agccuucgaa gccaccguca gaggcgcgaa acggauggca     1860

guacuaggcg acaccgccug ggacuucggc agcgucggcg gagcccucaa cagcuuaggc     1920

aagggaaucc accaaaucuu cggcgccgca uucaagagcc ucuucggcgg caugucgugg     1980

uucucccaaa uccucauagg cacucuacua auguggcuag gacucaacac gaaaaacggc     2040

agcauaagcc ugaugugccu cgcguuaggc ggaguccuca uauuccugag cacagccguc     2100

ucagcggacg ucgggugcag cguggacuuc agcaaa                               2136


<210>  2867
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long CDS opt

<400>  2867
agaauaauca acgcccgcaa agagaaaaag cgcagaggcg cagacacauc cguaggcaua       60

guaggccuac uccugacaac agcaauggcc gccgaaguaa cccgucgagg aucagccuac      120

uacauguauc uagaccgaaa cgacgccgga gaagcuauca gcuuccccac cacacucggc      180

augaauaagu guuacaucca aaucauggac cucggccaca ugugcgacgc aaccaugagc      240

uacgaaugcc caaugcugga cgaaggcgua gaacccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggaacc ugccaccaua agaaagggga agccagacgg      360

agccgacgug cgguaacgcu ccccagccac ucaacucgca aacuccaaac ccgcagccaa      420

acguggcucg aaagccggga auacaccaaa caccuaauca gaguggaaaa cuggauauuc      480

cgaaaccccg gauucgcgcu cgcagcagcc gcgauagcuu ggcugcucgg aagcuccacc      540

ucccagaaag uaauauaucu agucaugauc cuccucauag cacccgccua cagccuccgc      600

ugcauaggcg uauccaaccg agacuucgug gaaggcauga gcggcggcac cuggguagau      660

gucguacucg aacacggagg cugcgucacc guaauggccc aagacaaacc cacaguagac      720

auagaacuug ucacaaccac cguauccaac auggccgaag ugcgguccua cugcuacgaa      780

gccagcauau cagacauggc cucggacuca cgaugcccaa cccaaggcga agccuaccuc      840

gacaaacaaa gcgacaccca auacguaugc aaacgcacac ucguagacag aggauggggc      900

aacgggugcg gucuuuucgg aaaaggauca cucgucacau gcgcaaaauu cgccugcucc      960

aaaaagauga ccggaaaauc aauccaacca gaaaaccuag aauaccgcau caugcucagc     1020

guacauggcu cccaacacuc cggcaugaua gucaacgaca ccggacacga aaccgacgaa     1080

aaccgcgcaa aaguagaaau cacacccaac agcccacgag ccgaagccac ccugggaggg     1140

uucgggagcc uaggacucga cugcgaaccg cgaacgggac uagacuucuc cgaccucuac     1200

uaccuuacca ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauccca     1260

uuaccauggc acgccggcgc ugauaccggc accccacacu ggaacaacaa agaagcccuc     1320

gucgaguuca aagacgcuca cgcgaaacgc caaaccguag ucguacucgg cucacaagaa     1380

ggcgccgugc auaccgcacu agcaggggca cuagaagcag aaauggacgg cgcaaaaggc     1440

cgccucagcu cuggccaccu uaaaugcaga cuaaaaaugg acaagcuacg ccuaaagggc     1500

gugagcuacu cacugugcac cgcagccuuu accuucacca aaauccccgc cgaaacucuc     1560

cauggaaccg uaacagucga aguccaguac gccggcacgg acggaccaug caaagucccc     1620

gcacaaaugg ccguugauau gcaaacacug acacccgugg gaagacucau caccgccaac     1680

ccagucauca ccgaaagcac agaaaacuca aaaaugaugc uggaauuaga cccaccauuc     1740

ggcgacucau acaucgugau cggggucgga gaaaagaaaa uaacacacca cuggcaccgc     1800

agcggaucaa caauaggcaa agcauucgaa gccacaguca gaggggcaaa aagaauggcg     1860

guacuaggag acacagcaug ggacuucggc ucagugggcg gagcccucaa cucccuugga     1920

aaaggcauuc accaaaucuu cggggcagca uucaaauccc uauucggagg aauguccugg     1980

uucucgcaaa uacucaucgg cacgcuccua auguggcuag gacuaaacac caaaaacgga     2040

aguauauccc uaaugugccu cgcgcucgga ggcguacuaa uuuuccugag caccgcaguc     2100

agcgcagaug uaggcugcuc cgucgacuuc agcaag                               2136


<210>  2868
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long CDS opt

<400>  2868
cgcauaauca augcacgaaa ggagaaaaag cgcaggggag ccgacaccaa cguaggcaua       60

gucggccuac uucuaacaac agcaauggcg gccgaaguaa caagacgcgg aucagccuac      120

uacauguacc ucgaccgaaa cgacgccggc gaagccauca gcuuccccac cacccucggc      180

augaacaaau gcuacauaca gauaauggac cucggucaca ugugcgacgc cacaauguca      240

uacgaaugcc ccaugcuaga cgagggagug gaaccggacg acgucgacug cuggugcaac      300

accaccagca ccuggguagu cuacggaacc ugccaucaca agaaaggcga agccagaaga      360

ucccgacgcg cuguaacccu ccccagccac agcacucgca aacuccaaac caggucacaa      420

accuggcuag aaucucgaga guacacaaaa cacuuaauac gaguagaaaa uuggauguuc      480

cgaaaccccg gauucgcccu cgcggcagca gcaauagccu ggcuccucgg auccuccaca      540

ucgcaaaaag ucauauaccu uguuaugauc cuacuaaucg caccggcaua cagcauccgc      600

ugcaucggag ucucaaacag agacuucguc gaaggaaugu cgggcggcac augggucgac      660

gucguucuug agcacggagg augcgucacg gucauggcuc aagacaaacc uacaguagac      720

auagagcugg uuacaacaac cguguccaac auggccgagg uccgguccua cugcuacgag      780

gcauccauca gcgacauggc cuccgacuca cgaugcccaa cccaagggga ggcuuaccuc      840

gacaaacaaa gcgacacaca auacgucugc aaacgaacac ugguugauag gggauggggc      900

aacggcugcg gccuuuuugg caaaggaagc cuggucacau gcgccaaguu cgccugcagc      960

aagaaaauga cgggcaaaag uauacaacca gaaaaccugg aauaccgaau caugcucagc     1020

guccacgguu cccaacacuc cggcaugaua guaaacgaca caggccacga aacagacgaa     1080

aacagagcaa aagucgaaau cacccccaac agcccacggg ccgaagccac acugggcggu     1140

uucggcagcc ucggacucga cugcgaaccg cgcacgggac ucgacuucuc agaccucuac     1200

uacuuaacaa ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauuccc     1260

cuaccauggc augccggagc ggacaccggc acaccccacu ggaacaacaa agaagcacug     1320

gucgaguuca aagacgcaca cgccaaacgg caaacagucg ucguccuagg aucacaagaa     1380

ggagcagucc acacggcacu agcgggcgcc cuagaagccg aaauggaugg cgcaaaaggc     1440

cggcuaucaa gcggacaccu aaaaugcaga cuaaaaaugg acaaacuuag acucaaaggc     1500

guaagcuaca gcuuaugcac agccgccuuc accuucacga aaauccccgc agaaacacuc     1560

caugguacag ucacgguaga agugcaauac gccggcacag acgggccaug caaagucccu     1620

gcacaaaugg cagucgacau gcagacccua accccagucg gccgacucau aacggccaau     1680

ccagucauca cagagucaac cgagaacagc aaaaugaugc ucgagcucga cccgccauuc     1740

ggcgacagcu auaucgucau cggugucggc gaaaagaaaa ucacacacca uuggcacagg     1800

agcgguagca caauaggcaa ggcauucgaa gcaaccgucc gcggcgccaa acgcauggcc     1860

guccuaggag acaccgccug ggacuucgga ucaguagggg gcgcccucaa uagccugggc     1920

aaaggcaucc aucaaaucuu cggggcagcc uucaaaagcc uuuucggugg aauguccugg     1980

uucagccaaa uccucauagg cacccuccua auguggcucg gccucaacac gaaaaacggg     2040

uccauauccc ucaugugucu agcacuaggc ggaguccuca ucuuccucuc caccgcgguc     2100

ucggccgacg ucggaugcuc gguagauuuc ucaaaa                               2136


<210>  2869
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long CDS opt

<400>  2869
agaauaauca acgcccgaaa agagaaaaag cgccgaggcg ccgacacaag cgucggcauc       60

gucggacuuc uccuaacaac agccauggcc gcggaaguaa caagaagggg cagugcauac      120

uacauguacc ucgaccgcaa cgacgcagga gaagccauca gcuuccccac aacauuaggc      180

augaauaaau gcuacauaca gaucauggac uugggccaca ugugcgacgc cacaaugagc      240

uacgaaugcc ccaugcucga cgaaggcgua gaaccagacg acgucgacug cuggugcaac      300

acaacaagca cguggguggu auacggaacc ugccaccaca agaaaggaga agcaagacga      360

agccguagag cagugacacu ccccagccac ucgaccagaa aacugcaaac acgcagccaa      420

accuggcucg aaucacgaga auacaccaaa caccucaucc gagucgaaaa cuggauacua      480

cggaaccccg gauuugcacu cgcagcagcc gccaucgcau ggcuacucgg cucaucgacc      540

ucccagaaag ucauauaccu uguaaugaua cuccuaaucg cgcccgccua cagcauccga      600

ugcaucggcg ugucuaaucg agacuucgua gaaggcauga gcggaggcac augggucgac      660

gucguacugg agcacggagg cugcgugacg guaauggcac aggacaaacc cacagucgac      720

auagagcuag ucacaacgac cgucuccaac auggcagaag uacgcagcua cuguuacgaa      780

gccagcauaa gcgacauggc cagugacagc cggugcccaa cccaagggga agccuaccua      840

gacaaacaaa gcgacacaca auacguaugc aaaagaaccc ucgucgaucg aggcugggga      900

aacggaugcg gccucuucgg gaaaggaucc cucgugaccu gcgccaaauu cgcaugcagc      960

aagaaaauga ccggaaaaag uauacaacca gaaaaccuag aguaucgcau aaugcuauca     1020

guccacggcu cccaacacuc cgggaugaua gucaacgaca ccggccacga aacagacgaa     1080

aaucgcgcaa aagucgaaau caccccaaac agcccaagag cugaagccac ccuaggagga     1140

uucggauccc ucggccucga cugcgaacca cgaacaggcc ucgacuuuuc cgacuuauau     1200

uaccucacaa ugaacaacaa acacuggcua guccacaagg aaugguucca cgacauccca     1260

cuacccuggc acgcaggcgc agacacaggc accccacauu ggaacaacaa agaagcacuc     1320

guggaguuca aagacgcuca cgcaaaacgc caaacaguag uaguccuagg cucacaagaa     1380

ggugccgucc acacagcucu cgccggagcc cuagaagcag aaauggaugg agcaaaaggc     1440

cgccugucga gcggccaccu caaaugucga cuaaaaaugg acaaacuacg ccuaaaaggc     1500

gucagcuaca gcuuaugcac agcagccuuc accuucacaa aaauaccggc ggaaacacuc     1560

cacggcacgg ucacgguuga aguccaauac gccggcacag acggucccug caaaguccca     1620

gcacaaaugg cagucgacau gcaaacccua accccaguag ggcgucuuau cacagccaac     1680

ccagucauua ccgagucaac agaaaacagu aaaaugaugc uagaacucga cccacccuuu     1740

ggggacucgu acaucgucau cggaauagga gaaaagaaaa uaacccacca cuggcaccga     1800

agcggcucca ccauaggcaa agccuucgaa gcaacaguaa gaggagcaaa acgcauggca     1860

guacuuggag acaccgcgug ggacuucgga agcgucggag gcgcgcucaa cagccucggc     1920

aaaggcaucc accagaucuu cggcgccgcg uucaaaagcc uguucggagg aaugucaugg     1980

uucucccaga uacuaaucgg aacccuccua auguggcuag ggcugaacac gaaaaacgga     2040

ucgauaagcc uaaugugucu cgcccuagga ggcguacuaa uauuccucuc cacagcagua     2100

agcgcagacg ucggcugcuc cguagacuuc ucaaaa                               2136


<210>  2870
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long CDS opt

<400>  2870
aggauaauaa acgcacgaaa agagaaaaag cgaagaggag ccgacacauc cgucggaauc       60

gucggacugc uauuaacaac agccauggcc gcagaaguca cccgcagagg cuccgccuac      120

uacauguacc uagaccgaaa cgacgcgggu gaagcaaucu ccuuccccac cacacucggu      180

augaacaaau gcuacauaca gaucauggac cucggccaca ugugcgacgc aacgaugucc      240

uacgaaugcc caaugcuaga cgaaggcgua gaacccgacg acgucgacug cuggugcaac      300

acaaccagua caugggucgu auacgggacc ugccaccaca agaaaggaga agcccgaaga      360

ucccggcggg caguaacccu accgucacac uccacacgca aacuucaaac gcgaagccaa      420

accuggcuag agaguaggga auauacaaaa caccuaauaa gaguagagaa cuggauauuc      480

cgcaaccccg gauucgcgcu cgcggccgcc gcaauagcau ggcuacucgg cagcagcacc      540

agccagaaag uaauauaccu agucaugauc cuacucauag ccccggcuua cucaauccgc      600

ugcauagggg ucagcaaccg cgacuucgua gaaggcauga gcggaggaac auggguggau      660

guaguacuag agcacggcgg gugcguaacc guaauggcac aagacaaacc uaccgucgac      720

aucgaacucg uaagcacaac uguauccaac auggccgaag uaaggucuua cugcuacgaa      780

gccaccauau ccgacaucgc cuccgacucc agaugcccaa cgcaagggga agccuaccua      840

gacaaacaau ccgacacaca auauguaugc aaacgaaccc uagucgacag aggaugggga      900

aacggcugcg gacuauucgg caaaggaagu cugguaaccu gcgcaaaauu cgcaugcucc      960

aagaaaauga caggaaaauc cauacaaccc gaaaaccucg aauaccgcau caugcuaagc     1020

guccacggaa gccaacacag cggaaugauc gucaacgaca ccggacacga aaccgacgaa     1080

aaccgagcca aaguagaaau cacaccaaac agcccccgcg ccgaagccac ccucggcggc     1140

uucggcuccc uaggacugga cugcgaacca cgaaccggcc uagacuucag cgaccuauac     1200

uaccuaacca ugaacaacaa acacuggcua guccacaaag agugguucca cgacauccca     1260

uugcccuggc acgccggagc cgacaccggu acaccccacu ggaacaacaa agaagcccua     1320

guagaauuua aagacgccca cgcuaaaaga caaaccgcag ucguccucgg aucccaagaa     1380

ggcgccguac acacagcacu agcaggcgcg cucgaagccg aaauggaugg cgcgaaagga     1440

agacucagca gcgggcaccu caaaugccgg uuaaaaaugg acaaacuccg ccucaaaggc     1500

gucucauaca gccucugcac cgcagcuuuc accuucacua aaauccccgc ggaaacucua     1560

cacgggacag ugaccgucga aguccaauac gccggaacag acggcccaug caagguacca     1620

gcacaaaugg cagucgacau gcaaacccua accccgguag gaagauuaau aacggccaac     1680

ccuguaauaa ccgaaagcac cgaaaacagc aagaugaugc ucgaacucga ucccccuuuu     1740

ggcgacagcu acaucguaau cggcgucggc gaaaagaaaa ucacccacca cuggcaccga     1800

uccggaucca caaucggcaa agccuucgaa gcaacggucc ggggagccaa acgaauggcg     1860

guccugggag acacggcaug ggacuucgga uccguaggug gggcccuaaa cucguuaggc     1920

aaaggcaucc accagaucuu cggcgcagcc uucaaaucac ucuucggggg aaugagcugg     1980

uucucgcaaa ucuuaaucgg aacacuucua auguggcucg gccuuaacac caaaaacgga     2040

agcaucucgc ugaugugccu agcacuaggc ggaguccuca uauuccuaag cacagccgua     2100

uccgcagacg ugggcugcag cguugacuuu agcaaa                               2136


<210>  2871
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long CDS opt

<400>  2871
agaaucauaa acgcacgcaa ggaaaagaaa cggcgcggcg ccgacacauc cguaggcauc       60

gucggcuuac uccuaacaac agcaauggca gccgaaguaa caaggcgagg aagugccuac      120

uacauguacc uggacaggaa cgacgcagga gaagccauca gcuucccaac cacucucggc      180

augaacaaau gcuauauaca aaucauggac cuaggacaua ugugcgacgc aaccaugagc      240

uacgaaugcc ccaugcuaga cgaaggagug gaaccagacg auguagacug cuggugcaac      300

acaaccucaa caugggucgu auacggaacc ugccaccaca agaaagguga agcccgacgc      360

ucaagacgag cgguaacacu cccuagccac ucuacccgaa aacuccaaac ccgcagccaa      420

acauggcucg aaucccgaga guacacaaaa caccuaauac gaguagaaaa cuggauauuc      480

cgaaacccgg gauucgcccu agccgcagcc gcaaucgccu ggcuccuagg cagcagcacc      540

ucgcaaaaag ucaucuaccu agucaugaua cuacucauag cccccgccua cagcauccgc      600

ugcauaggag uaucgaaccg ggacuucgua gagggcauga gcggcggcac cugggucgac      660

guaguacuag aacacggcgg gugcguuacg gucauggcac aagacaaacc cacggucgac      720

aucgagcuag ucacgaccac cgucucaaac auggcagaag uccgcuccua cuguuacgaa      780

gcaucaauca gcgacauggc guccgacucc aggugcccga cccaaggaga agccuaucuc      840

gacaagcaaa gcgacacaca guauguuugc aagcgcaccc uugucgacag gggcuggggu      900

aacggcugcg gccucuucgg gaagggcucc cuagucacau gugcaaaauu cgcaugcagc      960

aagaaaauga ccggcaaauc cauccaacca gaaaaccucg aauaccgcau aauguuaagc     1020

guacacggcu cccaacauuc cggcaugauc guuaacgaca ccggacacga aacagacgaa     1080

aaccgagcaa aagucgaaau aacaccaaac agcccccgag cagaagccac ccuaggagga     1140

uucggaucac ucggacuaga cugcgaaccg cgaaccggac ucgacuucuc agaccuuuac     1200

uaccuaacaa ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauacca     1260

cugcccuggc acgccggcgc agacacagga acgccacacu ggaacaacaa agaagcccua     1320

gucgaguuca aagacgcaca cgccaagcga caaacagugg uaguacucgg aacccaagaa     1380

ggggccgucc acaccgcccu ggcaggagca cucgaagccg aaauggacgg ggcgaaggga     1440

cgccucuccu caggacacuu aaaaugcaga cuuaagaugg acaaacuccg acuaaaagga     1500

gucagcuaca gccuaugcac cgcagcauuc acguucacca aaauaccagc ugaaacacuc     1560

cacggcacgg ucacagucga aguccaauac gccggcacag acggaccaug caaaguaccc     1620

gcccagaugg ccguggacau gcaaacgcuc acccccgucg gacgccucau aaccgccaac     1680

cccgucauaa ccgaauccac cgaaaacagc aaaaugaugc uagaacucga cccucccuuc     1740

ggagacucau acaucgucau aggcgucggc gaaaagaaaa ucacgcacca uuggcacaga     1800

uccggaucaa caauaggcaa agcauucgaa gcaacaguga gaggggccaa gcgaauggca     1860

guccuuggag acacagcaug ggacuucgga agcguaggug gugcgcuaaa cucccuaggc     1920

aaaggaaucc accaaaucuu cggagcagca uucaaaagcc uuuuuggcgg aaugucgugg     1980

uucagccaaa uccuaaucgg gacgcugcua auguggcuag gacuaaacac gaaaaacgga     2040

uccaucucgc ugaugugccu agcacuaggc ggaguccuga ucuuccuuuc aaccgccguc     2100

uccgcagacg uaggcugcuc agucgacuuc uccaaa                               2136


<210>  2872
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long CDS opt

<400>  2872
cgaauaauca acgcccgaaa agaaaagaaa agacgagggg cagacacauc cgucggcaua       60

guagggcucc uccucacaac agccauggcc gcagaaguaa cccgacgagg cagcgcauac      120

uauauguacc ucgacaggaa cgacgccggu gaggcaauau cguuccccac gacccucggg      180

augaacaagu gcuauauaca gaucauggac cucggccaca ugugcgacgc aacaauguca      240

uacgaauguc caaugcucga cgagggagua gaacccgaug acguugacug cugguguaac      300

accaccagca cguggguugu cuacggaacc ugccaucaca agaaaggcga agcccgacgc      360

ucccgcagag ccgucacacu gcccucgcau ucaacaagaa aacuccaaac acgcucccaa      420

acauggcucg aaucccgcga auauacaaaa caccucaucc gcguagaaaa cuggauauuc      480

aggaaucccg gguucgcccu agccgccgca gcaaucgccu gguugcucgg cucaagcacc      540

agccaaaaag uaauauacuu agucaugauc cuacucaucg cccccgcgua cagcauccgc      600

ugcaucggag ucaguaaccg ggacuucguc gaaggcaugu ccggaggaac auggguggac      660

gucguacuag agcacggagg cugcgugacc guaauggcac aagacaaacc cacaguagau      720

auagagcuag uaacgacaac cguauccaac auggcagaag uucgaagcua cugcuaugaa      780

gcaagcauca gcgacauggc aagcgacucc cgaugcccca cccagggaga agccuaccua      840

gacaagcaau ccgacacaca auacguaugc aaacgaaccc ucgucgaccg cggaugggga      900

aacggcugcg gccuauucgg gaaaggaucc cuaguaacau gcgcaaaauu cgcaugcagc      960

aagaaaauga ccgguaagag cauccaaccc gaaaaccucg aauaccgaau aaugcucagu     1020

guacacggcu cccaacacag cggcaugauc guaaacgaca caggacacga aaccgacgaa     1080

aaccgugcga aaguggaaau aaccccgaac ucccccagag ccgaagcaac ccuaggcggc     1140

uucggaucac ugggccucga cugcgaacca agaaccggac uagacuucuc cgaccuauac     1200

uaccucacaa ugaacaacaa acacuggcuc guacacaagg aaugguucca cgacauaccc     1260

cuccccuggc acgccggagc agacacaggg acaccgcacu ggaacaauaa agaagcccug     1320

gucgaguuca aagacgcuca cgcaaaacgc caaacugucg ucguccuugg cagccaagaa     1380

ggcgccgucc acacagcacu cgccggugcc cucgaagccg aaauggacgg agccaaaggg     1440

cggcuaagca gcgggcaccu gaaaugccgg cuuaaaaugg acaaacuccg acugaaaggu     1500

guaucauaca gccuaugcac cgcagccuuc acauucacaa aaauacccgc agaaacacuc     1560

cacggaaccg ucacagucga acuccaauac gccggaaccg acggacccug caaagugcca     1620

gcccaaaugg caguagacau gcaaacgcuc acucccguag gccgccuuau aacugcgaac     1680

cccgucauaa ccgaaagcac agaaaacagc aaaaugaugc ucgaacucga ucccccguuu     1740

ggggacucau acaucgucau uggcgugggc gaaaagaaaa ucacgcacca cuggcaccgc     1800

ucaggcagca cuaucggaaa agcauucgaa gcaaccgucc gcggcgccaa gagaauggcu     1860

guacucggcg acaccgcaug ggacuucgga ucggucggag gggcccucaa cagccucggc     1920

aaaggaauac accaaaucuu cggcgccgcc uucaaaucuc uauucggagg gaugucgugg     1980

uuuucccaga uccugauagg cacccuccuc auguggcuag gccucaacac gaaaaacggc     2040

uccauuagcc uuaugugccu cgcccuaggc ggcguauuaa ucuuccucuc aacagcaguc     2100

agcgccgacg uagggugcag cgucgacuuu ucaaaa                               2136


<210>  2873
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long CDS opt

<400>  2873
cggaucauca acgcccgcaa agaaaagaag cgcagaggag cagaaaccuc cgucggcaua       60

gucggccugc uacuaaccac cgccauggcc gccgaaguca ccagacgagg cuccgccuac      120

uacauguacc ucgacagaaa cgacgccggc gaagccaucu ccuucccuac aacccucggc      180

augaacaaau gcuacauaca aauaauggac cuaggccaca ugugcgacgc cacuaugagc      240

uacgaaugcc ccaugcucga cgaaggaguc gaacccgacg acguggauug cuggugcaac      300

accacaucga caugggucgu guacgggacg ugccaccaca aaaagggaga agccagacga      360

ucacgcagag ccgucacacu ccccagccac agcacgagaa aacuacaaac acguagccaa      420

acguggcucg aaagccgcga auacaccaag caccucaucc gcaucgagaa cuggauauuc      480

cgcaaccccg gcuucgcacu agccgcggcc gccaucgcau ggcuacucgg aucgagcacc      540

ucacaaaaag ucauauaccu agucaugauc cuacuaauag cccccgcaua cuccauacgc      600

ugcauuggcg ucagcaaccg ggacuucgua gaaggcauga gcgguggcac augggucgac      660

gucguccucg aacacggagg cugcguaacc gucauggcuc aagacaaacc uacgguagac      720

auagaacugg uuaccacaac agucagcaac auggccgaag uccgcagcua uugcuacgaa      780

gcaagcauau cagacauggc cagcgacucc cguugcccaa cacagggaga ggcauaccua      840

gacaaacaau ccgauacaca guacguaugc aaacguaccc ucgucgaccg cgguuggggc      900

aauggcugcg gccuauucgg aaaaggaagc cucgucacau gugcaaaauu cgccugcagu      960

aagaagauga caggcaaauc aauccaaccc gaaaaccuag aauacagaau aaugcucucg     1020

guccacggaa gccaacacuc cggaaugauc guaaacgaca ccggacacga aaccgacgag     1080

aaccgcgcca aggucgaaau cacccccaac ucaccacgcg cggaagcaac acugggaggc     1140

uucggaucac ucggacucga cugcgaaccc cgaaccggau uagacuuuuc cgaccucuac     1200

uaccucacaa ugaacaauaa acacuggcuc guccacaagg aaugguucca ugacauacca     1260

cuacccuggc augccggcgc ggauacggga acgccccacu ggaacaauaa ggaggcccuc     1320

guggaguuca aagacgccca cgcaaaacgc caaaccguag uagugcucgg cagccaagaa     1380

ggagcugucc acaccgcccu ggccggagcc cucgaagccg aaauggacgg ggcgaaagga     1440

cgccucuccu cgggccaccu gaaaugccgc cuaaaaaugg acaaacuccg auuaaaaggg     1500

gucaguuaca gccuaugcac cgcggcauuc accuucacaa aaauaccagc agaaacacua     1560

cauggaaccg ucacagucga aguccaguac gccggcacag acgguccaug uaaagucccc     1620

gcacaaaugg ccgucgacau gcaaacacug acuccagucg gaagacucau caccgcaaac     1680

ccaguaauaa ccgaaucaac cgaaaacucc aaaaugaugc ucgaacucga cccaccauuc     1740

ggcgacuccu acauaguaau aggcgucgga gaaaagaaaa uaacacacca cuggcaccga     1800

agcggaucca ccauuggcaa ggcuuuugaa gcaaccguac gaggagccaa acgcauggcc     1860

gugcuaggag acaccgccug ggacuucggc uccgucggcg gcgcccuaaa cucccugggc     1920

aaaggcauac aucaaauauu cggcgccgca uucaaaucac ucuucggagg caugagcugg     1980

uucagccaga uccucauagg cacccuccuu augugguugg gccucaacac caagaacgga     2040

agcaucuccc ucaugugccu agcacucggc ggcguacuca uauuccucuc aacagccguc     2100

agcgccgacg uaggcugcag cgucgacuuc ucuaaa                               2136


<210>  2874
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long CDS opt

<400>  2874
agaauaauaa acgcacgaaa ggaaaagaaa cgccgaggcg cggacacaag cgugggaaua       60

gucggacucc uacucacaac ggcaauggca gccgaaguca cacgaagggg cucagcuuac      120

uacauguacc uggacagaaa cgacgccggg gaagcaauca gcuuuccgac cacacuagga      180

augaauaaau gcuacauaca aauaauggac cucggacaca ugugcgaugc aaccaugucg      240

uacgaaugcc caaugcuuga cgagggcgua gagcccgacg acgucgauug cugguguaac      300

accacgagca cauggguagu uuauggaaca ugccaccaca agaaaggaga agcacgcaga      360

agcagacgcg cugucacgcu cccaagucac agcacgcgca agcuccagac ccggagccag      420

accugguuag aaagccgcga auacaccaaa caccucauca gagucgaaaa cuggaucuuc      480

agaaacccag gguucgcacu agcagccgcc gccauagccu ggcuacucgg auccuccacg      540

agccagaaag uaauauaccu cguaaugaua cuccugaucg caccagcaua cucgauccgc      600

ugcauaggcg uaagcaaccg ggacuucgug gaagggaugu caggaggaac cugggucgau      660

guaguucuug agcacggagg augugugacg guaauggcuc aggacaaacc cacgguagac      720

auugaacucg ucaccaccac cgugagcaac auggcagaag ugcgaucaua cugcuacgag      780

gccuccauau ccgacauggc cagugacuca agaugcccaa cgcaaggaga agcguaccua      840

gacaaacaau cagacacgca guacgucugc aaacgaacgc uagucgaccg cggauggggg      900

aacgggugcg gacuauuugg caaaggcuca cuggugacau gcgccaaauu ugcaugcuca      960

aagaaaauga caggcaaauc cauacaaccc gagaaccucg aauaucggau caugcuaucc     1020

guacauggcu cacaacauag cggcaugaua gucaacgaca caggacacga aacagacgag     1080

aacagagcca aagucgaaau aaccccaaau agcccccgcg cggaagcaac ccucggagga     1140

uucggaagcc uaggacugga cugugaacca agaacaggcu uggacuuuag cgaccucuac     1200

uaccugacaa ugaacaacaa acacuggcua guccacaaag aaugguucca cgacauaccg     1260

cuaccguggc acgcaggggc cgacaccggc accccgcauu ggaacaacaa agaggccuua     1320

gucgaguuca aagacgcaca cgcaaaaaga caaacgguag uaguacucgg cucgcaagaa     1380

ggcgccguac acaccgcgcu cgcaggagcc cucgaagcgg aaauggacgg agcaaaaggu     1440

cgccugagcu ccggccaucu caaaugcaga cuaaaaaugg acaaacugag acucaaaggg     1500

guaucuuaca gccuaugcac ggcagccuuc acauucacaa aaaucccugc cgagacacua     1560

cacgggacgg ucacagucga gguacaauac gccggaaccg acggaccaug caagguaccc     1620

gcccaaaugg ccgucgacau gcaaacacua acaccagucg ggcgccuaau cacagcuaau     1680

cccgucauca cugaaucaau cgaaaacagc aaaaugaugc uagaacuaga uccgcccuuc     1740

ggcgacagcu acaucguaau cggcgucgga gaaaagaaaa ucacacacca cuggcauaga     1800

agcgggagca caauaggcaa agcauucgaa gccacaguac gcggagcgaa acgaauggca     1860

guccucggcg acaccgcgug ggauuucggg agcgucggcg gcgcacucaa cucgcucgga     1920

aaaggaaucc accaaaucuu cggagccgca uucaaaagcc uauuuggcgg caugagcugg     1980

uuuagccaaa uacucaucgg aacacuccug auguggcucg gacucaacac gaaaaacggc     2040

agcauaaguu ugaugugccu cgcccucgga gggguacuca uauuucugag cacagccguc     2100

agcgcagacg uagggugcag cguggacuuc agcaag                               2136


<210>  2875
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long CDS opt

<400>  2875
cgcaucauaa acgcccgcaa agaaaagaaa cgcagaggcg cagacacauc agucggcauc       60

guaggacuuc uacuaacuac cgcgauggca gcagagguaa cccgccgcgg auccgcauau      120

uacauguacc ucgaccgcaa cgacgcgggc gaagcaaucu cauucccaac aacccuggga      180

augaacaaau guuacaucca aaucauggac cucggacaca ugugcgacgc aaccaugucc      240

uacgaaugcc ccaugcuaga cgaaggcguc gaaccagacg acgucgacug cuggugcaac      300

accaccagca cauggguugu auacggcacg ugucaucaca agaaaggaga agcgcgccga      360

agccgacgcg cagugacgcu gcccucccac uccacccgca aacugcaaac gcgcucccag      420

acauggcuag agagccgaga guacaccaaa caccuaaucc gaguagaaaa cuggaucuuc      480

cgcaacccag gcuucgcacu cgccgccgcc gcaaucgccu ggcuacucgg aucuagcacc      540

agccaaaaag uaaucuaccu cgucaugauc cuccugaucg cucccgccua cagcauccgg      600

ugcaucgggg ucagcaacag agacuucgua gaaggaaugu ccgggggaac auggguagac      660

gucguccuag aacacggggg cugcguaacu gucauggcuc aggacaaacc cacagucgac      720

auagaacuug ucaccaccac cgucagcaau auggcggaag uacgcagcua cuguuacgaa      780

gcaagcauau cagacauggc cucugacagc cguugcccga cccaaggaga agcauaucuc      840

gacaaacagu ccgacaccca auacgucugc aaacgaaccc ucguagaccg aggauggggg      900

aacggaugug gacucuucgg caaaggaagc cucguaaccu gcgcaaaauu cgcauguucc      960

aagaaaauga ccggcaaauc aauccaacca gaaaaccucg aauaccgaau caugcucagc     1020

guccacggau cccagcacuc cggcaugauc guaaacgaua cuggccacga aaccgacgaa     1080

aaccgagcca aagucgaaau aacccccaau agcccacgcg ccgaagccac ccuaggcgga     1140

uucggcuccc ucggccucga cugcgaacca cgcaccggcc uagacuucag cgaccucuau     1200

uaccuaacca ugaacaacaa acacuggcug guccacaaag aaugguucca cgacaucccc     1260

cucccuuggc acgccggggc ggacacagga accccacacu ggaacaauaa agaagcacuc     1320

gucgaguuca aagacgcaca cgcuaaaagg caaaccgucg uaguacuagg cagccaagaa     1380

ggagcaguuc acaccgcauu agccggcgca cuagaagccg aaauggacgg agucaaagga     1440

cgccucuccu ccggacaccu aaaaugccgc cucaaaaugg acaaacuacg ccuaaaagga     1500

guaaguuauu cacucugcac agcagccuuc acauucacca aaaucccagc agaaacccua     1560

cacggcacag ugacagugga aguccaauac gcaggcaccg acggcccaug caaagucccu     1620

gcacagaugg ccgucgacau gcaaacccuc acaccagucg gcaggcucau caccgcuaac     1680

ccaguaauca ccgaaucaac cgaaaacuca aagaugaugc ucgaacucga cccgcccuuu     1740

ggggacuccu acaucgugau cgggguuggg gaaaagaaaa ucacccacca cuggcaccgu     1800

ucaggaucaa caaucggcaa agcauucgaa gcaaccguac gaggcgcaaa aagaauggcc     1860

guacuaggcg acacagcaug ggacuucggc ucagucggcg gggcccuaaa cucccucggc     1920

aaaggcauac accaaaucuu cggagcagca uucaaguccc ucuucggcgg caugagcugg     1980

uucagccaaa uccucaucgg cacacugcua auguggcugg gacucaacac caaaaacgga     2040

ucgaucucau ucaugugccu ggcacucggu ggcgugcuca uauuccucag cacagccguc     2100

uccgcagacg ucggcugcag cguagauuuc ucaaag                               2136


<210>  2876
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long CDS opt

<400>  2876
aggauaauaa acgcccgaaa ggaaaagaaa cgacgaggag ccgacacauc cgucggcauc       60

guaggacucc uccuuaccac cgccauggcg gcggagguca cacgcagagg gucagccuac      120

uacauguacc uagaccgcaa cgacgccggc gaagcgauca guuuucccac cacgcuggga      180

augaacaaau gcuacaucca aaucauggac cuaggacaca ugugcgacgc cacaauguca      240

uacgaaugcc ccaugcucga cgaaggcguc gaaccagacg acgucgacug cuggugcaac      300

acgaccucca caugggucgu guacggaacc ugucaccaca agaaaggcga agcccgacga      360

ucacgcagag ccgucacccu acccucccac agcacccgca aacuucaaac cagaucacaa      420

accuggcucg aaucccgaga guauacaaaa caccucauac gagucgaaaa cuggaucuuc      480

cgcaacccag gauuugcccu ggcggccgcu gccauagccu ggcuacucgg cuccucaacc      540

ucacaaaagg ucauauaccu cgucaugauc cuccuaaucg cccccgccua cagcauccgc      600

uguauaggag ucucaaacag agacuuugua gaaggaaugu caggcgggac cuggguugac      660

gugguacugg aacacggcgg cugcguaacu gucauggcgc aagacaaacc cacaguggac      720

auagagcuag uaaccaccac cguaagcaac auggcagaag uacgcuccua cugcuacgaa      780

gcaucgaucu ccgacauggc cucagacucc cgcugcccca cccaaggcga agcauaccuc      840

gacaaacaau cagacaccca auacgucugc aagcgcaccc ucguagaccg cggauggggc      900

aacggcugcg gccuguucgg gaaaggcucc cuaguaaccu gcgccaaauu cgcaugcagc      960

aaaaagauga cuggcaaauc cauccaaccc gagaaccucg aauacagaau caugcucuca     1020

guccacggau cccaacacuc cggcaugaua gucaaugaca cgggacacga aacagacgaa     1080

aaccgcgcga aagucgagau caccccaaac ucaccccgag cagaagcaac ccuuggagga     1140

uucggcagcc uaggacucga cugcgaaccc agaaccggcc uagacuucuc cgaccucuac     1200

uaucucacca ugaacaacaa acacugguua guccauaagg aaugguucca cgacaucccc     1260

cuccccuggc acgcaggcgc ugacaccggc acaccccacu ggaacaacaa agaggcacuc     1320

gucgaguuca aggacgccca cgcaaaacga caaacaguag ucguacucgg cucccaggaa     1380

ggcgcaguac acacagcccu agcaggcgca cucgaagcgg aaauggacgg agcaaaaggu     1440

aaacuuucaa gcggccaccu caaaugccga cugaaaaugg acaagcugcg ccuaaaagga     1500

gucagcuaca gucucugcac ggccgcauuc acauuuacaa aaauaccagc cgaaacgcua     1560

cacggcaccg ucaccgucga aguccaauac gccggcaccg acggccccug caaaguaccc     1620

gcacaaaugg ccguagacau gcaaacccuc acaccgguag gacgccucau caccgcaaac     1680

cccgucauua cagaauccac cgaaaacuca aaaaugaugc ucgaacuaga ucccccguuu     1740

ggugacuccu acaucgucau cggaguagga gaaaagaaaa uaacacacca cuggcaccgc     1800

agcggaucga caaucggaaa agccuucgag gcaaccgugc gcggcgcaaa acgaauggcc     1860

guccucggag acaccgccug ggacuucggc uccgucggcg gggcacuaaa cagccuagga     1920

aaaggaaucc aucagauauu cggagcagcc uucaaaucac uguucggagg cauguccugg     1980

uucucccaaa uacugauagg cacacuacuc augugguuag gccucaacac caaaaacgga     2040

agcauaagcc ucaugugccu cgcccucggc ggcguccuca uauuccucuc cacagcgguc     2100

uccgcagacg ugggaugcag cguagacuuc agcaaa                               2136


<210>  2877
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long CDS opt

<400>  2877
cgaaucauca acgcacgcaa agaaaaaaaa cggcgaggcg ccgacaccag cguaggcaua       60

guaggacucc uacuaacaac agccauggcc gccgaaguaa cccgacgcgg cuccgccuac      120

uacauguauc ucgaccggaa cgacgccggc gaagcaauau ccuuccccac aacccucggc      180

augaacaaau gcuacauaca aaucauggac cucggccaua ugugcgacgc aacaauguca      240

uacgaaugcc ccaugcucga cgaaggcguu gagccagacg acgucgacug uuggugcaac      300

accacaagca ccuggguagu cuacggaaca ugccaccaua aaaaaggcga agccagacga      360

uccaggagag cgguaacucu gccaucacac uccacacgaa aacuacaaac cagaucccaa      420

acauggcucg aaagccgcga auacaccaag caccucaucc gcguagagaa cuggauuuuc      480

cgaaaccccg gauucgcccu agccgccgcc gccaucgccu ggcuucuggg auccucgacc      540

agccaaaaag uaaucuaccu cgucaugauc cucuuaauag cccccgcgua cagcauacga      600

ugcaucggag uaucgaaccg cgacuucgua gagggaauga gcggcggcac auggguagac      660

guaguacucg agcacggagg uugcguaaca gucauggcac aagacaaacc uacaguggac      720

aucgaacucg uaacgaccac cgucagcaac auggcggaag ugcgcucaua cugcuacgaa      780

gcaucgauaa gcgacauggc cuccgacucc cgcugcccaa cgcaaggcga agccuaccuc      840

gauaaacagu ccgacaccca guacgucugc aaacgcaccu uagucgacag gggaugggga      900

aacggcugcg gccuauucgg caaaggcagc cuugucaccu gcgccaaauu cgcgugcagc      960

aaaaaaauga caggaaaauc cauccaaccc gaaaacuuag aguaccgcau aauguuauca     1020

guccacggau cccaacacuc aggaaugauc gucaacgaua caggccacga aacagacgaa     1080

aaccgcgcca aagucgaaau cacacccaac uccccccgcg cagaagccac ccuaggcggg     1140

uucggaagcc ucggacuaga cugcgagccc cgcacaggac uugacuucag ugaccuauac     1200

uaccuaacaa ugaacaacaa gcacuggcuc guccacaaag aaugguucca cgacaucccc     1260

cuacccuggc acgcaggagc cgauaccggc acaccucacu ggaauaacaa agaagcgcua     1320

gucgaauuca aagaagcaca cgcaaaacga caaacaguag uaguguuagg cucacaagaa     1380

ggcgcagucc acaccgcgcu agccggagcc cuagaagcgg aaauggacgg cgcaaaaggc     1440

cgccuaucaa gcggccaucu aaagugucga cuaaaaaugg acaaacuccg acugaaaggc     1500

guauccuaua gccugugcac cgccgcauuc accuucacaa aaauccccgc agagacccuu     1560

cacggaacgg ucacuguaga aguccaguac gcaggcaccg acggaccaug caaagucccc     1620

gcacaaaugg ccguugacau gcaaacccua acaccagucg gaagacucau aaccgccaac     1680

ccagucauca cagaaaguac cgaaaacagc aaaaugaugc ucgaacucga ccccccguuc     1740

ggcgacagcu acaucgugau cggcguaggc gaaaaaaaaa uaacgcacca cuggcauaga     1800

ucaggcagca ccauaggaaa agccuucgaa gcaacaguac gcggcgcaaa acgaauggcc     1860

guacucggcg acacggcaug ggacuucggc uccgugggcg gagcgcugaa uagucugggc     1920

aaagguaucc accaaaucuu uggggccgca uuuaaauccc ucuucggcgg caugucaugg     1980

uucucacaaa uccuaauagg aacacuucug auguggcugg gacucaacac gaaaaacggc     2040

uccaucuccc ugaugugccu agcacucggc ggcguacuga uauuccuauc cacagcaguc     2100

ucagcagacg uaggcugcuc ggucgacuuc agcaaa                               2136


<210>  2878
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long CDS opt

<400>  2878
cgaaucauca acgcaaggaa agaaaagaaa agacggggcg cggacacaag cgucgggauc       60

gucggucuac uacucacaac cgcaauggcc gcugaaguca cccgcagagg cagcgcauau      120

uacauguacc uggacagaaa ugacgcagga gaggcaauca gcuuccccac aacgcuaggc      180

augaacaaau gcuacauaca aaucauggac cuaggacaca ugugcgaugc gacaaugagc      240

uaugagugcc ccaugcucga cgaaggaguc gaaccagacg augucgacug cuggugcaac      300

accacaagca cgugggucgu auacggcaca ugccaccaca aaaagggcga agcacgacga      360

agcagacgag ccgucacacu gccaucacac uccacacgca aacuccaaac acggagccaa      420

acauggcugg aaucccggga auacaccaaa caccucaucc gggucgaaaa cuggauauuc      480

agaaacccag gauucgcccu cgcagcugcc gccauagcau ggcuccuagg cucaagcaca      540

ucacaaaaag ugauauaccu uguaaugaua cuacucaucg cgcccgccua cucaauacga      600

ugcauagggg uaagcaaucg cgauuucgug gaagguaugu cagguggcac cuggguagac      660

gucgugcucg agcacggagg auguguaacg guaauggcac aggacaaacc cacggucgac      720

aucgagcucg ucaccacgac gguaagcaac auggccgaag uacgcucaua cugcuacgag      780

gcguccauau cagacauggc aucagacagc cgcugcccca cccagggaga ggcauaccug      840

gacaaacaaa gugauacaca auacguaugc aaacggacgc ucgucgacag aggcuggggc      900

aacggaugug gacuauucgg caaaggaagc cuaguaacgu gcgccaaauu cgccugcagc      960

aagaaaauga caggaaaauc cauacaacca gaaaaccuag aauaccgaau aaugcucuca     1020

guacacggcu cacaacacag uggcaugaua guaaacgaca ccggccauga aacugacgaa     1080

aaccgcgcca aagucgaaau cacccccaac agcccgcgag cagaagcaac acucggagga     1140

uucggauccu uaggacucga cugcgaacca cgcacaggac ucgauuucag cgaccucuac     1200

uaccucacga ugaacaauaa acauuggcua guccacaaag aaugguucca cgacaucccc     1260

cuacccuggc acgcaggcgc cgacacagga acaccccacu ggaacaacaa agaagcacug     1320

guagaguuca aagacgcaca cgccaaacgc cagacagucg ugguccuagg aucacaggag     1380

ggggcagucc acaccgcacu cgccggagcc cuagaagccg agauggacgg agccaaagga     1440

agacucagcu cgggccaccu caaaugcaga cuaaaaaugg auaaacuaag acucaaaggu     1500

gucuccuacu cccucuguac agccgccuuc acauucacca aaauaccagc cgagacgcua     1560

cacggcaccg uaacagucga ggugcaguac gcaggcgcgg acggaccaug caaggugcca     1620

gcccaaaugg ccguagacau gcagacccuc acaccgguag gccgacuaau aaccgccaau     1680

cccguaauaa ccgaauccac cgaaaacuca aaaaugaugu uagaacugga cccgcccuuc     1740

ggggacagcu acauagucau cggaguggga gaaaagaaaa ucacacacca cuggcacaga     1800

aguggaagca caauagggaa agcguuugag gccaccguac gcggcgccaa acgcauggca     1860

guccuaggag acaccgcaug ggacuucggc agcgucggcg gagcacucaa cucacugggc     1920

aagggaaucc accaaauuuu cggcgcagca uucaaaagcc uauucggagg aaugagcugg     1980

uucucacaaa uacugaucgg cacacuacua auguggcugg gacugaauac caaaaacgga     2040

agcaucaguu uaaugugccu ugcacuagga ggcguacuga uauuccuaag cacagccguc     2100

uccgcagacg uggggugcag cguagacuuc agcaaa                               2136


<210>  2879
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long CDS opt

<400>  2879
agaauaauaa acgcagggaa agagaagaaa cgaaggggag ccgacacaaa cgucggcauc       60

gucggacuau ugcuaacaac ggcgauggca gccgaaguaa cacgccgcgg aucagcguac      120

uacauguacc uggaccgaaa cgacgccgga gaagcaaucu ccuucccaac cacgcucggc      180

augaacaaau gcuacaucca aauaauggac cuaggacaca ugugcgacgc caccaugucc      240

uacgaaugcc cgaugcucga cgaaggcguc gaaccagacg augucgauug cugguguaau      300

accacgagua caugggucgu cuacggcaca ugccaucaca agaaaggaga agcacgcaga      360

agcagacgcg ccguaacacu accaagccau uccacaagaa aacuacagac aaggagccaa      420

acauggcugg aaagccgcga auacacaaaa caccuaauca gaguggaaaa cuggaucuuc      480

aggaauccag gauucgcacu ggccgcggcg gcaauagcgu ggcuccuagg aagcaguaca      540

ucacaaaaag uaaucuaccu cguaaugaua uugcugaucg cgccagccua cucuauaagg      600

ugcauaggag ucagcaaccg ugacuucgua gaaggaaugu ccggcggcac cugggucgac      660

gucgugcucg aacacggagg gugugucacc guaauggccc aagacaaacc caccguagac      720

auagaacugg ucacaacgac cguaagcaac auggcagaag ugcgcagcua cugcuacgag      780

gccucaauca gcgacauggc aagcgacucc cgcugcccaa cacaaggcga agcauaccug      840

gacaaacaga gcgacaccca guaugucugc aaaaggaccc ucgucgaccg aggaugggga      900

aacggaugcg gccuauucgg caaaggaucc cuagugacgu gcgccaaauu cgcaugcagu      960

aagaaaauga ccggcaaaag cauacaacca gaaaacuuag aauacaggau caugcucuca     1020

gugcacggau cacaacacag cggaaugaua gucaacgaca ccggccacga aacggacgaa     1080

aacagagcca aagucgaaau uacacccaac aguccacgcg cagaagcgac ccucggaggg     1140

uucgggagcu uaggacucga cugcgaacca cgaacgggac ucgacuucag cgaccuguau     1200

uaccuaacaa ugaacaacaa acauuggcua gugcacaaag agugguucca cgacaucccc     1260

cuaccauggc acgccggggc cgacacaggc acaccccauu ggaacaacaa agaagcucua     1320

gucgaguuca aagacgcaca cgcaaaaagg caaaccgucg ugguacuugg cagccaagaa     1380

ggcgcagucc acacugcacu agcaggagcg cuagaagccg aaauggacgg agcaaaaggc     1440

cgccuauccu cggggcaccu caaaugcaga uugaaaaugg acaaacuaag acugaaagga     1500

guaucauacu cccucugcac cgcugccuuc accuucacca aaauacccgc agaaacccuc     1560

cacggaacgg uaacagucga aguacaguau gccggaaccg acggccccug caagguccca     1620

gcccaaaugg ccgucgacau gcaaacacua accccaguug gccgccuaau cacggccaac     1680

ccaguaauca cagaaucaac cgagaacucc aaaaugaugc ucgaacucga cccacccuuc     1740

ggagacagcu acaucguaau cggcgucggc gaaaagaaaa uaacccacca cuggcaccgc     1800

agcggaagca caauuggcaa agcguucgaa gcaacgguca gaggcgcacg cagaauggca     1860

guucucggcg auacagcaug ggauuucggc agcguaggag gggcgcucaa cucccucggg     1920

aaaggcauac aucaaaucuu uggggcagcg uucaaaagcc uguucggcgg caugucaugg     1980

uucagucaaa uccuaauagg aacacuccua auguggcucg ggcuaaacac gaaaaacggg     2040

uccaucagcu uaaugugccu cgcacucgga ggaguacuca ucuuccuauc cacagcugua     2100

agcgcagacg ucggaugcuc aguggacuuc ucaaaa                               2136


<210>  2880
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long CDS opt

<400>  2880
cgcauaauca acgccagaaa agagaagaaa cgacgaggag cagaaaccag cguggggaua       60

gugggccugc uccuaaccac agcaauggcu gccgaaguga cacgaagagg aagcgccuau      120

uacauguacc uggacagaaa agacgcagga gaagccauca gcuuuccaac cacacucggg      180

augaacaaau gcuacaucca gauaauggac cuaggccaca ugugcgacgc aaccauguca      240

uacgaaugcc ccauguuaga cgaaggcgug gagccggacg acguagacug cuggugcaac      300

accaccagca cuuggguggu guacggaaca ugccaccaca agaaaggcga agccagacgc      360

agcagacgag ccgucacgcu ccccagccac uccacacgga aacuacagac acgaagccaa      420

acauggcuag aaagccgcga auacaccaaa caccuaauca gagucgagaa cuggaucuuc      480

cgcaaucccg gcuucgcauu agccgcagcc gcaauagccu ggcuacuagg cagcagcaca      540

ucccaaaaag uuaucuaucu ugugaugaua cugcuaauag cccccgcgua cucaauccgg      600

ugcaucggcg ucucaaaccg ggacuucgua gaaggcaugu caggcggcac cugggucgac      660

gucguacuag aacauggagg augcgucacc guaauggccc aagacaaacc caccguagac      720

aucgaacucg ucacgacuac cgucaguaac auggcagaag ugcgauccua cugcuacgaa      780

gccagcaucu ccgacauggc cuccgacagc cgcugcccaa cacaaggcga agcauaccuc      840

gacaaacaaa gcgacacaca auacgugugc aaacgaaccc uagucgaccg cggauggggu      900

aacggaugcg gccucuucgg aaagggcagc cuagugaccu gcgcaaaguu cgcaugcagc      960

aagaaaauga caggcaaaag uauccagcca gaaaaucugg aguacagaau aaugcuuuca     1020

guacacggau cacaacacuc aggaaugauc gucaacgaca caggccauga aacagacgag     1080

aaccgcgcga aaguagaaau aacccccaac agccccagag cagaagcaac acucggaggg     1140

uucggcagcc uaggacucga cugcgaacca cgaaccgggc uagacuucag cgaccuauac     1200

uacuuaacaa ugaacaauaa acacuggcuc guucacaaag aaugguucca cgacaucccc     1260

cuaccauggc augcaggagc cgacaccgga acgccccacu ggaacaacaa ggaggccuua     1320

guagaguuca aggacgcaca cgcgaagcga caaaccguag ucguccuagg aucccaagaa     1380

ggagccguac acacagcacu ggccggcgcc cucgaagcag aaauggacgg ggcaaaaggc     1440

cgacuaagca gcggccaccu aaagugcaga cugaagaugg acaaacugag acucaaaggc     1500

gucuccuaca gccucugcac cgccgcauuc acauucacca agauaccagc ggaaacacua     1560

cacggaaccg ucaccguaga agugcaauac gcaggcacug acggccccug caaaguccca     1620

gcacaaaugg caguagacau gcaaacacuc acaccaguag gacgacucau caccgccaac     1680

ccaguuauca ccgagucaac agaaaacuca aaaaugaugc ucgaacucga cccgcccuuc     1740

ggcgacagcu acaucguaau aggaguggga gaaaagaaaa uaacgcacca cuggcaccgg     1800

agcggcagca caauaggaaa agcauucgaa gcaacaguca gaggcgccaa aagaauggcc     1860

guccucggcg acacggcaug ggacuucggg agcgucggag gcgcauuaaa cucccugggg     1920

aaaggaaucc accaaauuuu cggagccgcc uucaaaagcc uauucggcgg gaugucaugg     1980

uucagccaaa uccucauugg gacccuacua auguggcuag gccugaacac gaagaacgga     2040

agcauaucac uaaugugccu agcacuaggg ggaguccuaa uauuccuauc cacggcggua     2100

agcgcagacg uaggaugcuc cguagacuuc agcaag                               2136


<210>  2881
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long CDS opt

<400>  2881
cgaaucauca acgcgagaaa agaaaagaaa agacguggag cggacacguc cgucggaauc       60

gucggacuau ugcuaacgac ggcuauggcc gcggaaguca cacgcagagg cagcgccuac      120

uacauguacc ucgaccgcaa cgacgcuggc gaagcgauca gcuuccccac cacccuugga      180

augaacaagu guuacaucca gauaauggac uuaggacaca ugugcgacgc caccaugucc      240

uacgaaugcc cuaugcuuga cgaaggcguc gagccagacg acguggauug cuggugcaac      300

acaaccagca cauggguagu auacggaacc ugccaucaca agaaaggcga agcccgaagg      360

aguagacgag ccguaaccuu accaucgcac ucaacacgaa aacuacaaac ccgcucccaa      420

acauggcuag aaucccgcga auacacaaaa caccucaucc gcgucgaaaa cuggauauuc      480

cgcaaccccg gauucgcacu agccgccgcc gcaaucgccu ggcuacucgg aagcucaacc      540

ucgcaaaaag ucauauaccu cguaaugaua cugcuaauag caccggcaua cuccauacga      600

uguaucggcg uguccaacag ggacuucguc gaaggaaugu ccggaggaac cugggucgac      660

gugguacuag agcacggagg cugcgucaca gugauggccc aggacaaacc aacagucgac      720

auagagcuag ucacaacaac cguguccaac auggccgaag uaagguccua cugcuacgaa      780

gccagcauaa gcgacauggu aucagauucg cgaugcccca cccaaggcga agcauaccuc      840

gacaaacaaa gcgacaccca guacguaugc aaacgcacgc ucgucgacag aggauggggc      900

aacggaugcg guuuauucgg caaaggaagc cuaguaaccu gcgcaaaauu cgccugcagu      960

aagaaaauga ccggaaaauc cauacaacca gaaaaccuag aauaccgcau caugcucucc     1020

guccacggau cacaacacag cggaaugaua gucaacgaca cgggacacga aaccgacgaa     1080

aacagggcca aaguagaaau aaccccaaac aguccccgug cagaagcaac acucggcggg     1140

uucggcucac ucggccucga cugcgaacca agaaccggcc uagacuucuc cgaccucuac     1200

uaccuaacaa ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauaccc     1260

cuaccauggc acgccggcgc cgacaccggc acuccacacu ggaacaacaa ggaagcccuc     1320

guagaguuca aagacgcaca cgcuaaacgc caaaccgucg uaguccucgg aagccaggaa     1380

ggagccgucc acaccgcccu ggcaggggcc cuggaagcug aaauggacgg agcaaaaggc     1440

agacucucaa gcggccaccu aaagugccga cucaaaaugg acaaacucag acuaaaggga     1500

guaagcuaca gccucugcac agccgcauuc acauucacca aaaucccagc cgaaacucua     1560

cacggcacag ucaccguaga aguacaauac gccggaacag acggaccgug uaaaguacca     1620

gcucaaaugg ccguggacau gcaaacacug acacccgucg gaagacucau aaccgccaac     1680

ccagucauaa ccgaaucgac cgaaaacagc aagaugaugc uagaacuaga cccgcccuuc     1740

ggagacagcu acaucguaau cggcguaggg gaaaagaaaa uaacccacca cuggcacaga     1800

ucaggcucca caauaggaaa agccuucgaa gccacaguac gaggcgccaa acgaauggcu     1860

guauuaggug acacggcaug ggacuuugga ucaguaggcg gcgcccuaaa cucacuagga     1920

aaaggcaucc accaaaucuu cggggcagcc uucaagucgc ucuuuggggg cauguccugg     1980

uucucgcaaa uccucaucgg gacccuccua auguggcuag gacucaacac caagaacggu     2040

ucgauauccc ugaugugccu cgcucucgga ggaguccuca ucuuccucuc cacugccguc     2100

agcgccgacg uuggcugcuc ggucgauuuc uccaaa                               2136


<210>  2882
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long CDS opt

<400>  2882
agaaucauaa acgcaagaaa agaaaagaaa agaagaggcg cggauaccaa ugucggaauc       60

gugggacucc uacuaacaac ggcaauggca gcggaaguaa cccgacgagg cagcgcauac      120

uacauguacc ucgacagaaa cgacgcagga gaagcaauca gcuuccccac cacucuaggc      180

augaacaaau gcuacaucca aauaauggac cucgggcaca ugugcgacgc gacaaugucc      240

uacgaaugcc ccaugcugga cgaaggcgua gaacccgacg acgucgacug cuggugcaac      300

augacgagca cauggguagu auacgggacg ugccaccaca agaaaggcga agcacgacga      360

agccgccggg caguaacccu cccaagccac agcacgcgca aacuacagac ucgcucacaa      420

accuggcucg agagccgaga auacacaaag caccuaaucc gcguagaaaa cuggaucuuc      480

cgaaaccccg gcuucgcacu agcagcagca gcgauagcgu ggcuacuggg cucaagcacc      540

agucagaaag ucaucuaccu gguaaugaua cuacuaauag caccagccua cagcaucagg      600

ugcauaggcg uaagcaaccg agacuucgua gaaggaauga gcggaggcac cuggguggac      660

gucguucucg aacacggagg gugcgucaca guaauggccc aagacaaacc cacaguggac      720

aucgagcuag uaacaaccac agucagcaac auggcagaag uccgcucaua cugcuacgaa      780

gccuccaucu ccgacauggc cagugacuca cgcugcccaa cacaaggaga agccuaccuc      840

gacaaacaau cagacacaca guacgucugc aaacgcacac uggucgaccg cggaugggga      900

aaugggugcg gacuauucgg aaaaggaucc cucguaaccu gcgccaaauu cgccugcagu      960

aagaaaauga caggcaaauc aauccagccg gaaaaccuag aauaccgcau caugcucagc     1020

guccacggca gccaacacag cgguaugaua guaaacgaca caggacacga aacagaugaa     1080

aaccgcgcaa aagucgagau cacacccaac agcccccgcg ccgaagcaac ccuagguggg     1140

uuuggcagcc ugggacuaga cugcgaacca cgaaccggac ucgacuucuc cgaccuauac     1200

uaccuaacua ugaacaauaa acacuggcuc guacauaagg aaugguucca cgacauccca     1260

cuaccauggc acgcuggagc cgacaccgga accccgcacu ggaacaacaa agaagcgcuc     1320

guagaguuca aagacgcaca cgccaaacga caaacagucg uuguacucgg uagccaggaa     1380

ggagcaguac acacagcacu cgccggagca cuagaagcag aaauggacgg agcaaaagga     1440

cgacugagca gcggccaccu gaagugcaga cucaaaaugg acaagcuccg ccucaaagga     1500

gucagcuaca gccucugcac cgccgcguuc accuucacaa aaauaccagc ggaaacacuu     1560

cacggaacgg uaacagugga aguacaauac gccggaacag acggacccug caaaguccca     1620

gcccagaugg ccgucgacau gcaaaccuua accccagucg gccgccuaau caccgcaaac     1680

cccgucauca ccgaauccac cgaaaacucc aaaaugaugc uggaacuaga cccacccuuu     1740

ggagacuccu acaucguuau aggaguaggc gagaaaaaga ucacccacca cuggcaccgc     1800

uccggaagca cgaucggcaa agccuucgaa gccaccgucc ggggagccaa acgcauggca     1860

guacuaggag acacagcaug ggacuucgga agcgugggcg gggcccugaa cagccuaggc     1920

aaaggaaucc accagauauu cggcgccgcc uucaaaagcc uauucggcgg cauguccugg     1980

uucucgcaaa uacuuaucgg cacacuauua auguggcucg gacucaacac aaagaacggc     2040

ucaaucagcc uaaugugucu cgcucuggga ggaguccuaa uauuccucag cacggcaguc     2100

agcgcggacg ucggcugcuc agucgacuuc uccaaa                               2136


<210>  2883
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long CDS opt

<400>  2883
cgcauaauca acgcccgaaa agagaaaaag cgacgcgggg ccgacacgag cgucgguaua       60

guaggccuac uucugaccac agccauggcg gccgaaguca cgcgccgggg cagcgccuac      120

uacauguacc uagaccgaaa cgacgcagga gaagcaauca gcuucccaac cacgcuaggc      180

augaacaaau gcuacauaca aaucauggac cuaggucaca ugugcgacgc aaccaugagc      240

uacgaaugcc cuaugcuaga cgaaggaguc gaacccgacg acguggacug cuggugcaac      300

accacaagca caugggucgu cuacggaacc ugccaucaca aaaagggcga agcacgcaga      360

agccgacgcg ccgucaccuu gccaagccac agcacccgga aacugcaaac aagaagccaa      420

acguggcuag aaagucgcga auauaccaaa caucucauac gcgucgaaaa cuggaucuuc      480

cgaaaccccg gcuucgcacu cgcagcggcc gcaauagcau ggcuccuagg guccagcaca      540

agccaaaaag uuaucuaccu cguaaugauc cuacucaucg cuccagcaua cuccauacga      600

ugcaucggcg ucagcaaccg agauuucguc gaaggaaugu ccggaggcac cuggguagac      660

gucguacucg agcacggcgg cugcgucacc gucauggccc aagacaaacc aacugucgac      720

auagaacucg ucaccaccac cgucucaaac auggccgaag ugcgcagcua cugcuacgaa      780

gcgagcauca gcgacauggc cagcgacucc agauguccca cucaaggaga agccuaccuc      840

gacaaacaau ccgacaccca auacgugugc aaacgcacgc uagucgaccg aggaugggga      900

aauggaugcg gacucuuugg aaaagguucg uuaguaaccu gcgccaaauu cgcaugcucc      960

aaaaagauga caggcaaauc aauacagccg gaaaaccucg aauacagaau caugcucagc     1020

guccacggca gccaacauag cggaaugauc guaaacgaca cgggacacga aacagaugaa     1080

aauagggcaa aagucgagau caccccuaac aguccccgag ccgaggcggc acuaggcgga     1140

uucggaucac uaggacucga cugcgagccc cgcacuggcc uggacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa acacuggcua gugcacaagg aaugguucca cgacaucccc     1260

cuacccuggc acgccggcgc cgacaccgga acuccccauu ggaacaacaa agaagcacug     1320

gucgaauuua aagacgcaca cgcgaaacga caaacagucg uaguacucgg aucccaagaa     1380

ggugccgucc acacagcacu agccggagcg cuggaggcag agauggaugg cgccaagggc     1440

cggcucuccu ccggccaccu aaagugucgu cuaaaaaugg acaaacuccg ccuaaaaggc     1500

gugucguaca gccuaugcac cgcagccuuc accuucacca aaauccccgc cgagacacua     1560

cacggaaccg ucacagucga aguccaauac gccggcacag acggcccgug caaagugccg     1620

gcccaaaugg caguggacau gcagacccua acaccagucg gacggcuaau cacagccaau     1680

cccguaauca cagaaagcac agaaaacagc aaaaugaugu uagaacuaga ucccccauuc     1740

ggcgacagcu acauagucau aggcgucgga gagaagaaaa uaacacacca cuggcacaga     1800

agcggcagca caaucggaaa agcauuugaa gcaaccgucc gcggagcaaa aagaauggca     1860

guacugggcg acaccgcaug ggacuucgga ucaguaggcg gcgcacuaaa cucauuaggc     1920

aaagggauac accaaauauu cggagcugca uucaaaagcc ucuucggggg caugagcugg     1980

uucucacaaa cacucaucgg cacccuacug auguggcugg gacuaaauac caaaaacgga     2040

agcaucucgc ucaugugccu cgccuuagga ggaguccuaa ucuuccucag caccgcugua     2100

agcgccgacg ucggcugcag cgucgacuuc ucaaaa                               2136


<210>  2884
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long CDS opt

<400>  2884
cgcauaauca augccaggaa agaaaagaaa aggcgcggcg cagaaaccuc agugggaauc       60

gucggacuac uacugaccac agcaauggcc gccgagguga cacgacgcgg aucugccuac      120

uacauguacc uagaccgaaa cgacgcuggc gaagcgaucu ccuuccccac aacccucgga      180

augaauaaau gcuacauaca aauaauggac cuaggccaca ugugugacgc cacaaugucc      240

uacgaaugcc caaugcucga cgaaggugug gaacccgacg acguggacug cuggugcaac      300

acaacgucaa cguggguagu auacggaacc ugccaccaca agaaaggaga agcaagaaga      360

agccgacgag cuguaacacu cccgagccac agcacgcgaa aacuccaaac cagaagccaa      420

acguggcuag aaagcagaga auacaccaaa caccuaauac gcgucgagaa cuggauauuu      480

cgaaaccccg gauucgcacu cgcagccgcc gcaauagccu ggcuacuagg caguucaacc      540

agucaaaaag ucaucuaccu aguuaugaua cuacucaucg cacuagcaua uagcauccga      600

uguauagggg ucucaaaccg cgacuucgua gagggaauga gcggcggcac cuggguagac      660

gucguccucg aacacggcgg augcguuacc guaauggcgc aggacaaacc caccguggac      720

auagagcuag uuaccacgac aguaagcaac auggcggaag ucagaucaua cugcuacgaa      780

gcaagcauca gcgacauggc aagcgacagc agaugcccaa cgcaaggaga agccuaccuc      840

gacaaacaga gcgacacaca auacgucugc aaacgcacgc uuguagaccg aggauggggc      900

aacggaugcg gacuguucgg aaaaggcuca uuagucacau gcgccaaauu cgcgugcagc      960

aagaaaauga ccggaaaaag uauccaaccc gaaaaccucg aauaccgaau caugcucucc     1020

guccacggau cacagcacag cggaaugaua guaaacgaca caggacacga aaccgaugaa     1080

aaccgggcga aaguagaaau cacuccaaac uccccuagag cggaagccac acuaggcggc     1140

uucggaagcc uaggccucga cugcgaacca cgcacgggcc ucgacuucag cgaccucuac     1200

uaccuuacca ugaacaacaa acacuggcug guacacaaag aaugguucca cgacaucccc     1260

cuaccauggc acgcaggagc agacacaggc acaccccacu ggaacaauaa agaggcacuc     1320

gucgaguuca aagacgccca cgcaaaacga caaaccgucg ucguccuagg aagucaagaa     1380

ggcgcggucc acacggcacu cgccggugcg cuagaagcag agauggacgg cgcgaaagga     1440

cgacuaagcu ccggacaucu caaaugcagg cucaaaaugg acaaacuccg gcugaaagga     1500

guaagcuacu cgcucugcac cgcugccuuc acauucacca aaauccccgc cgaaacacuc     1560

cacggcaccg ucaccgucga gguacaauac gcaggcacag auggaccgug caaaguccca     1620

gcacaaaugg ccguagacau gcaaacacug acacccgucg gacgacucau caccgcuaac     1680

cccgucauca ccgaaaguac cgagaacagc aaaaugaugc uagaacucga cccgcccuuc     1740

ggagacucau acaucguaau cggagucggg gaaaagaaaa uaacacacca uuggcaccga     1800

uccggcucca cuauaggaaa agcguucgaa gccacgguac gcggugccaa acgaauggca     1860

guccucggcg acaccgccug ggacuucgga agugucggag gcgcauuaaa cucccucggc     1920

aaaggaauac accaaaucuu cggcgccgca uucaaaagcc uauucggagg aauguccugg     1980

uucucccaaa uacugauagg gacacuccuc auguggcucg gucuaaacac caaaaacgga     2040

agcaucuccc ucaugugccu ugcccuugga ggaguacuca ucuucuuguc aacagccgua     2100

agugcugacg ucggcugcag cgucgacuuc uccaaa                               2136


<210>  2885
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long CDS opt

<400>  2885
cgaaucauaa acgcuaggaa agagaagaaa cggcgaggag cggacacauc cgugggaaua       60

guuggccugc uccuaacaac cgcaauggcg gcagaaguaa cgcgccgagg cuccgccuac      120

uacauguacc uagaccguaa cgaugcuggg gaggcaauau ccuucccuac cacccucggc      180

augaacaaau gcuacaucca aauaauggac cucggacaca ugugcgacgc cacaaugucc      240

uacgagugcc cgaugcuaga cgaaggcguc gaaccagacg acgucgacug cuggugcaau      300

acaaccagca ccugggucgu auacggcacu ugccaccaca agaaaggaga agccagacgc      360

agcagacgag ccgucacgcu cccaagccac uccacccgca aacuacagac ccgaucccaa      420

acauggcucg aaagccgaga auacacaaaa cacuuaaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcacu cgcagcggcc gccauagccu ggcuccucgg gagcagcacc      540

ucccagaagg uaaucuaccu ggugaugaua cuccuaauug cacccgccua cuccauacgg      600

ugcauaggca uauccaaccg agacuucguc gaaggcaugu caggaggaac cugggucgac      660

gucguucuag aacacggagg cugcguaacc gucauggccc aagacaaacc aacagucgac      720

auagagcuag ucaccacaac cgucuccaac auggccgaag ucagaaguua cugcuacgag      780

gcaagcauca gcgacauggc cagcgacagc cgcugcccca cacaaggaga agcauaccuc      840

gauaaacaau cagacaccca auacgucugc aaacgcacac ugguagacag aggauggggc      900

aacggcugcg gucucuucgg gaaaggcuca cugguaaccu gcgccaaauu cgccugcucc      960

aagaaaauga cagggaaauc cauacaacca gaaaaccuag aauaccgcau aaugcuaucu     1020

guccacggau cacaacacuc cggcaugaua guaaacgaca ccgggcacga aaccgacgaa     1080

aacagagcca aaguagaaau cacccccaac agcccccgcg cggaagcaac acucggagga     1140

uucggcauac uaggacugga cugcgaaccc agaaccggac uggauuucag cgaccuuuau     1200

uaccucacaa ugaacaauaa acacuggcug guccauaaag aaugguucca cgacauaccc     1260

uuacccuggc augcgggagc agacacaggc acaccgcacu ggaacaauaa agaagcccuc     1320

gucgaguuca aagacgccca cgcaaaaagg caaaccgucg uaguccuggg cucccaagaa     1380

ggagccguac acaccgcacu cgccggcgcc cuagaagcag agauggacgg ggccaaaggc     1440

cgacuaagcu ccggccaccu caaaugccgu cuaaaaaugg acaaacuacg cuuaaagggc     1500

gugucauacu cacucugcac agcagccuuc acauucacca aaauaccggc agaaacacug     1560

cacggcacag ucacagugga agugcaauau gccggcacag acggcccaug uaaaguccca     1620

gcccaaaugg caguagacau gcaaacgcua acaccagugg gccgccuaau aacagccaac     1680

cccgucauca cagaaaguac agaaaacuca aaaaugaugc uagagcuaga ucccccauuu     1740

ggcgacucau acauaguaau aggcgucggc gaaaagaaaa ucacccauca cuggcaccga     1800

ucaggaagca caauuggaaa agccuucgaa gcgacaguaa gaggagccaa acgaauggca     1860

guacugggag acacagccug ggacuucgga agcgugggcg gagcacugaa cucgcuagga     1920

aaaggcaucc accagaucuu cggcgcagcc uucaagucac ucuucggcgg caugagcugg     1980

uucucccaga uacucaucgg cacgcuccuc auguggcuag gacuaaacac gaaaaacggu     2040

agcaucucac ugaugugccu cgcccucgga ggaguccuca uauuccuauc gacagccgua     2100

agugccgacg ugggaugcuc cgucgacuuc uccaaa                               2136


<210>  2886
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long CDS opt

<400>  2886
cgcaucauaa acgccagaaa agaaaagaaa aggcguggcg ccgacacauc cguaggaaua       60

gucggucuac uacucaccac agccauggca gccgaaguca caaggagagg cucagcauac      120

uacauguacc ucgaccgcaa cgaugccgga gaggccauuu cauucccaac aacccuagga      180

augaacaaau gcuacaucca aauaauggac cucggccaua caugcgacgc cacaaugucc      240

uacgaaugcc cuaugcucga cgaaggagua gaaccggacg acgucgacug cuggugcaac      300

accaccucaa ccugggucgu guacggaaca ugccaccaca agaaaggaga agccagacgc      360

agccgccggg cagugacccu ccccucccac ucgauccgca aacuccaaac acgcagccaa      420

accugguuag aaucacgaga auacaccaaa caucuaauca gggucgaaaa cuggaucuuc      480

cgaaacccag gcuucgcacu cgccgcagcc gcaauagcgu gguuacucgg aagcuccaca      540

agccaaaaag ucaucuaccu cguaaugaua cuucuaauag caccagccua cagcauccgc      600

ugcaucggag ucagcaaccg agacuuuguc gagggcauga gcggcggcac cuggguggac      660

gucguacucg aacacggcgg cuguguuacg gucauggccc aggacaagcc cacagucgau      720

auagaacucg ucacuacaac cgucagcaac auggcugaag uacgaagcua cugcuacgag      780

gccuccauaa gcgacauggc aagcgacucc cgcugcccca cccaaggcga agcauaccuu      840

gauaaacaau ccgacacaca auauguaugc aaacgcacac ucguagacag agggugggga      900

aacgggugcg gacucuucgg caaaggaucc cucguaaccu gcgccaaauu cgccugcucc      960

aaaaagauga ccggaaagag cauccaaccc gaaaaccucg aauaucggau caugcucucc     1020

guccacggau cccaacauag cggaaugauc guaaacgaca cagggcacga aaccgacgaa     1080

aaccgcgcaa aaguagaaau caccccgaac agcccccgcg cggaagccac ccucgggggc     1140

uucggaagcc ugggccucga cugcgaaccc cgaacaggac uagacuucuc cgaccucuac     1200

uaccuaacca ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauaccc     1260

cuaccauggc acgcaggcgc cgacacgggc acaccgcacu ggaacaacaa agaggcauua     1320

gucgaauuua aggacgcgca cgcaaaacgc cagacaguag ucgugcuggg cucacaagaa     1380

ggcgccguac acaccgcccu ugccggcgca cuagaagcag agauggacgg cgcaaaaggc     1440

agacuauccu ccggccaccu aaaaugcaga cuaaagaugg acaaacuccg gcugaaagga     1500

guguccuaca gccuuugcac cgcugccuuc acguucacaa aaauccccgc cgagacccuc     1560

cacggaacgg uaacaguaga gguccaguac gcaggcaccg acggaccgug caaaguccca     1620

gcccaaaugg ccgucgacau gcaaacgcuc acccccgucg gccgacugau aaccgcgaac     1680

ccagucauca ccgaguccac cgaaaacucc aaaaugaugc uagaacuaga cccaccauuc     1740

ggggacucau acaucgugau aggaguaggc gaaaagaaaa ucacccacca cuggcaucgu     1800

uccggcucca caaucggcaa agcauucgaa gccacaguca gaggcgccaa acgaauggcc     1860

gugcucggag acaccgccug ggacuucgga agcguaggcg gcgcccucaa cucccucgga     1920

aaaggaaucc accaaaucuu cggggccgcc uucaaguccc ucuucggcgg aaugucaugg     1980

uucucucaga uacucaucgg aacucugcuc auguggcuag ggcuuaacgc caagaauggc     2040

ucgaucucac ucaugugccu agcauuagga ggaguccuca uauuccuaag caccgcggua     2100

uccgcagacg uagggugcag cgucgacuuc aguaaa                               2136


<210>  2887
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long CDS opt

<400>  2887
cgaauaauaa acgccagaaa agaaaagaaa cgacgaggag ccgacaccuc aguaggcauc       60

guaggacuac uacucacgac agcaauggca gcagagguaa cccgccgcgg gagugcauac      120

uacauguacc uugacagaaa cgacgcggga gaagcgauau ccuucccaac aacgcucggg      180

augaacaaau gcuacaucca aauaauggac cucgggcaua ugugcgacgc aacaaugucg      240

uacgaaugcc caaugcuaga cgaaggcgua gaaccagacg acgucgacug cuggugcaac      300

acaaccagca ccugggucgu auacggcacg ugucaccaca agaaaggaga agcccaacgc      360

uccaggcgag ccgucacgcu ccccagccac agcacccgaa aacuccaaac ccgcagccag      420

acauggcucg aaucaagaga auacaccaaa caccuaauac gagucgaaaa cuggaucuuc      480

cgcaaccccg gauucgcccu cgcagccgca gcgauagcgu ggcuccuggg cagcaguacc      540

agccagaaag uaauauaccu ugucaugaua cuccucauag cccccgccua caguauacgc      600

ugcauaggcg uaagcaaccg cgacuucgua gaaggaauga gcggcggaac augggucgac      660

guggucuuag aacacggcgg cugugugaca guaauggcac aagacaagcc caccguagac      720

auagaacugg ucacaacaac aguaagcaac auggcagaag uccgcucgua cugcuacgag      780

gcuucaaucu ccgacauggc aucagacuca aggugcccaa cucaaggaga agcauaccuc      840

gacaaacaau ccgacaccca auacgucugu aaacgaacac uugucgaccg cggauggggg      900

aacggaugcg gccucuucgg gaaaggaucc cucguaaccu gcgccaaauu cgcgugcucg      960

aagaaaauga cgggaaaaag cauacagccc gaaaaccuug aauacagaau aaugcucagc     1020

guccacggau cacaacacag cggcaugaua gucaacgaca caggccacga aaccgaugaa     1080

aaccgggcca aaguagaaau cacgccaaac uccccacgag ccgaagcgac acucggaggc     1140

uucggauccc uggggcuaga cugcgaaccu cgaacaggcu uggacuucag cgaccuguac     1200

uaccuaacaa ugaacaacaa acacuggcug guacacaaag aaugguucca cgacauccca     1260

cuccccuggc acgccggagc agacaccgga accccucacu ggaacaacaa agaagcacuc     1320

gucgaguuca aagacgcaca cgccaaaaga caaacaguag ugguccucgg gagccaagaa     1380

ggagcaguac acacagcccu cgcaggcgcc cucgaagcag agauggacgg agcaaaagga     1440

cgccugagcu caggacaccu aaaaugcaga cuaaaaaugg acaaacuccg acuaaaaggc     1500

gucucuuacu cacuauguac agcagcguuu accuucacaa aaaucccagc ggaaacauua     1560

cacggaaccg ucaccgucga aguacaauau gccggaaccg acggcccaug caaagucccc     1620

gcacaaaugg cagucgacau gcaaacacuc acccccgucg gacgccugau cacagcgaac     1680

ccaguaauca ccgagucaac ggagaacagc aaaaugaugc uagagcugga cccgccauuu     1740

ggagauuccu acauagucau cggcgucgga gagaagaaaa ucacacacca cuggcaucgc     1800

agcggaagca caauagguaa agcauucgaa gcgacagucc guggagcaaa aaggauggcg     1860

guccucggcg auaccgcaug ggacuucggc agcguaggcg gagcccuaaa cagccucgga     1920

aaaggcauac accaaaucuu cggcgccgcc uucaaaagcc uauucggcgg aaugagcugg     1980

uucagccaaa uccuaauagg cacacuccuc auguggcucg gccucaacac caaaaacgga     2040

agcaucagcc ucaugugccu agcccucggc ggcguccuaa ucuuccuguc aacagccgug     2100

agcgcagacg ucggcugcag cguggacuuc ucaaaa                               2136


<210>  2888
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long CDS opt

<400>  2888
cgcauuauca acgcgcgaaa agagaaaaag agacgaggcg cagacaccuc cguaggcauc       60

gucggccuac ugcucaccac cgccauggca gcagaaguca ccagaagagg uucagcguau      120

uacauguacc ucgaccgaaa cgacgcaggg gaagcaauca gcuucccaac cacccuagga      180

augaacaagu gcuacauaca aaucauggac cugggacaca ugugcgacgc caccauguca      240

uacgaaugcc cgaugcucga cgaaggagua gaacccgaug acgucgacug cuggugcaac      300

accaccagca ccuggguagu cuacggaacc ugccaccaca agaaaggcga agcacgacgc      360

ucgcgcagag cagucacccu cccaucacac ucaacaagaa aacuacaggc cagaucacaa      420

acauggcucg aaucccgaga guauaccaaa caccugaucc gcgucgaaaa cuggauauuc      480

cgaaaucccg gcuucgcacu ggccgccgcc gccauagccu ggcugcucgg cagcagcacc      540

agccaaaaag ugaucuaccu cgucaugaua cugcucauag ccccagccua cucaauacgc      600

ugcauaggag uaucaaacag agacuucguc gaggggaugu caggaggcac cuggguagac      660

gucguccucg aacacggcgg augcgugaca gucauggccc aggacaaacc cacaguagac      720

aucgagcuag ucacaacaac agucucaaac auggccgaag uucgcucaua cuguuacgaa      780

gccagcauca gcgacauggc gagcgacuca cgcugcccga cccaaggaga agccuaccug      840

gacaaacagu ccgacacgca guacgucugc aagagaaccc uaguagacag gggcuggggc      900

aacggcugcg ggcuauucgg gaaagguagc cuuguaaccu gcgccaaauu cgccugcucc      960

aagaaaauga ccggcaaauc gauccagccc gaaaaucuag aauaccgcau aaugcucucc     1020

gugcacggca gccaacacuc aggcaugauu guaaacgaca cuggccacga gacggacgaa     1080

aaccgcgcaa aagucgaaau cacaccaaac uccccacgcg cggaggcaac ccucggaggc     1140

uucggaagcc uaggccucga cugugaaccc cgcaccggcc uagacuucuc agaccuuuau     1200

uaccucacaa ugaacaacaa acacuggcuc guucacaaag aaugguuuca cgacaucccg     1260

cuccccuggc acgcaggcgc cgacacagga acaccccacu ggaacaacaa agaagcccua     1320

gucgaguuca aagacgcaca cgcaaaacgc caaaccgucg ucguacucgg cucgcaagaa     1380

ggagcaguac acaccgccuu ggcaggcgcc cucgaagccg aaauggacgg cgccaagggc     1440

cggcucagcu ccggccaccu caaaugccgc cuaaaaaugg acaaacuacg acuaaaagga     1500

guaagcuaca gccuaugcac cgcugcauuc acuuucacaa aaauacccgc agaaacccua     1560

cacggcaccg ucacggucga aguucaauac gccggcaccg acggacccug caaaguccca     1620

gcccaaaugg caguagacau gcagacacuc accccagucg gaagacuaau caccgcaaac     1680

ccaguaauaa cagaauccac cgaaaacagc aaaaugaugc ucgaacucga cccgccguuc     1740

ggagacagcu acaucguaau cggcguaggc gaaaagaaaa uaacacacca cuggcaccga     1800

uccggcucca ccaucggcaa ggccuucgaa gcaaccguac gaggagccaa acgaauggca     1860

guacucggag auacggcuug ggauuucggc agcgucgggg gagcccuaaa cucacuagga     1920

aaaggcaucc aucaaaucuu cggagcggcu uuuaaaaguc uauucggagg caugaguugg     1980

uucagccaga uacuaaucgg cacacuacua auguggcucg gccucaacac caaaaacgga     2040

uccaucucac uaaugugucu cgcccuaggc ggaguacuaa uauuccucag caccgccguc     2100

agcgccgacg uaggaugcag uguggacuuc ucaaag                               2136


<210>  2889
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long CDS opt

<400>  2889
cgcaucauca acgcgcgcaa ggagaagaaa cgccgaggcg cagacacauc cgucggaauc       60

guagggcuac uccucacaac cgcaauggcc gcagaaguga cacgacgcgg aucugcauac      120

uauauguacc uagacagaag cgacgcagga gaagcaauua gcuucccaac gacccucggg      180

augaacaaau gcuacauaca aauaauggac cucggacaca ugugcgacgc aacgaugucu      240

uacgaaugcc ccaugcuaga cgaaggggug gaacccgacg acgucgacug cuggugcaac      300

accacaagca cguggguagu cuacggaacc ugccaccaca agaaaggcga agcccgcagg      360

agcaggagag ccguaacacu ccccucccac agcacccgaa aacugcaaac gagaucacaa      420

acguggcucg aauccagaga auauacaaaa caccuaauca gagucgagaa cuggauauuc      480

agaaacccag guuucgcccu agcagcagca gcaauagccu gguuacuagg aagcagcacc      540

agccaaaaag uaauauaccu aguaaugaua cuccucauag ccccagcaua cagcauacgg      600

uguaucggag uaagcaaccg agacuucguc gaaggaaugu cgggaggaac auggguagac      660

guaguacucg aacacggcgg cugcguaaca gucauggccc aagacaaacc aacaguagac      720

auugagcuag ugacuacaac agucagcaac auggcggaag uccgcucgua cugcuacgaa      780

gcaucgauca gcgacauggc aagcgacagu agaugcccca cacagggcga agccuacuua      840

gacaaacaau cagacaccca auacguaugu aaaaggaccc uagucgaucg cggauggggc      900

aacggaugcg ggcuauuugg caaagguuca cucguaaccu gcgcgaaauu cgcgugcagu      960

aagaaaauga caggcaaaag uauacaacca gaaaaccucg aguaccgcau aaugcuaucg     1020

guccacggcu cccaacacag cggcaugauc gucaacgaca cgggacacga aaccgacgaa     1080

aacagagcaa aagucgagau cacaccaaac ucaccacgag cagaggcaac ccuuggcgga     1140

uucggcaguc ucggacucga cugcgaaccg cggacaggcc ucgacuucuc agaccuauau     1200

uaccuaacca ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauacca     1260

cuaccauggc acgccggcgc cgacaccggc acaccacacu ggaacaacaa agaagcacuc     1320

guagaguuca aagacgcgca cgcgaaacga caaacaguag uaguacuagg cucacaagaa     1380

ggagcagucc acacagcacu ggccggcgca cuagaagccg aaauggacgg cgcaaaagga     1440

cgucuaagua gcggucaccu aaaaugccga cuaaaaaugg auaaacuacg ccuaaaaggg     1500

gucucauacu cgcucugcac cgcagcauuc accuuuacga aaaucccagc cgaaacccug     1560

cacgggacag uaaccguaga aguccaauac gccggcaccg acggcccaug caaaguacca     1620

gcccaaaugg ccgucgacau gcaaacccug accccggugg gaagacugau cacagccaac     1680

ccagucauca cagaaagcac ggagaacagc aaaaugaugc uggaacuuga cccacccuuc     1740

ggcgacagcu acaucguaau agggguggga gaaaagaaaa uaacacauca cuggcaccgc     1800

ucagguagca caaucggcaa agcauucgag gcaacaguca gaggagcaaa acggauggca     1860

guccuaggag acacagccug ggauuucgga agcguaggag gggcgcucaa uucacuaggc     1920

aagggcaucc accaaauauu cggagccgcc uucaagucac uguucggcgg aaugagcugg     1980

uucagccaaa uccucaucgg cacacuauua augugguuag gacugaacac uaaaaacgga     2040

uccaucagcc ucaugugccu cgcgcugggc ggcguacuga uauuccugag cacagccgua     2100

agcgccgacg ucgggugcuc aguagacuuu ucaaaa                               2136


<210>  2890
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long CDS opt

<400>  2890
cgcauaauaa acgccagaaa agaaaagaag agaaggggaa cagacacauc cgugggcauc       60

gucggccucc ugcucacaac agccauggca gccgaaguca cccgaagagg cagcgcauac      120

uacauguacc uagacagguc agacgcaggc gaagcaauau ccuucccaac aacacuagga      180

augaacaaau gcuacaucca gauaauggac uuaggacaca ugugcgacgc aacgauguca      240

uacgaaugcc ccauguuaga cgaaggaguu gaaccagacg acguggacug cuggugcaac      300

acuacaucaa cguggguggu guacggaaca ugccaccaca agaaaggaga agcgcgcaga      360

uccagacgag cagugacccu accaagccau agcacccgaa aacuccaaac aagaagccaa      420

accuggcugg agagcaggga auauaccaaa caccucauaa gggucgagaa cuggauauuc      480

agaaacccag gcuucgcacu cgccgcagca gccauagccu ggcuccuagg cucaagcaca      540

agccaaaaag uaauauaucu cgugaugauc cuacuaaucg caccagcaua cucaauacgc      600

ugcaucggcg ucaguaacag ggacuucgua gaagggaugu cgggaggcac auggguggac      660

guuguacucg agcacggagg augcgucacc gucauggcac aagauaaacc cacaguggac      720

aucgaacucg uaacuaccac agugucuaac auggcagaag uacgaucaua cugcuacgaa      780

gcgucgauau cagacauggc cagugacucc cggugcccga cacaaggaga agcauaccuc      840

gacaaacaga gcgacacaca auacguaugu aaaagaacgc ugguagaccg cggauggggc      900

aacggcugcg gacucuucgg caaaggaucc cucguaaccu gcgcaaaauu cgcgugcuca      960

aagaaaauga ccggaaagag cauacaacca gaaaaccucg aguaccgcau aaugcucuca     1020

guccacggaa gccagcacag cgggaugaua gucaacgaca caggccacga aacagacgaa     1080

aaucgagcaa agguagaaau cacaccgaac agcccccgcg ccgaagccac gcuaggagga     1140

uucggcuccc uaggacucga cugugaaccg cgcacaggac uagacuucuc cgaccuauac     1200

uaccucacaa ugaacaacaa acacuggcua gugcacaagg aaugguucca ugacauaccc     1260

cuccccuggc acaccggagc cgacaccgga acaccacacu ggaacaacaa agaagcccug     1320

guagaguuca aagacgcaca cgcaaaaaga caaacaguag ugguccuagg cagucaagaa     1380

ggcgcuguac acacagcacu agcaggagca cuagaagccg aaauggacgg cgcaaaagga     1440

agacugucgu caggacaccu caagugccga uuaaaaaugg acaaacuaag acugaaagga     1500

gucucguaca gccucuguac cgcggccuuc acauucacca agauaccagc cgaaacacug     1560

cacggaacag uaacaguaga agugcaguac gccgggacgg acggaccaug caaagugcca     1620

gcacaaaugg ccguagacau gcaaacacuc acgccagucg gccgccugau caccgccaac     1680

cccgucauaa cagaagggac cgagaacucg aaaaugaugc uggaguugga cccacccuuc     1740

ggagacagcu acaucguaau cggagugggc gagaaaaaga uaacgcacca cuggcaccgc     1800

aguggaagca ccaucggcaa agccuucgaa gccaccgucc gcggcgcaaa gagaauggca     1860

guccuaggcg acacagcaug ggacuucgga agcguaggag gcguucugaa cagccuaggc     1920

aaaggaaucc accaaaucuu cggcgcagcc uucaaaucac ucuucggggg caugucaugg     1980

uucagccaaa uacuaauagg cacccuacuc auguggcucg ggcuaaacac uaaaaacgga     2040

agcaucagcc uaaugugccu cgcccuggga ggcguacuaa ucuuccuaag cacagcaguu     2100

ucagccgacg ugggaugcag cgucgacuuc ucaaag                               2136


<210>  2891
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long CDS opt

<400>  2891
cgaauaauua acgcuagaaa agaaaagaaa agacgaggag ccgauacauc cguagggauu       60

gucggacuac uccuaacaac agcgauggcg gcagaaguga cacggcgugg cagcgccuac      120

uacauguacc uggaccgaaa cgacgcaggc gaagccaucu cauucccaac aacacuuggc      180

augaauaaau gcuacaucca aauaauggau cuaggccaca ugugcgacgc gacaaugagc      240

uacgaaugcc caaugcuaga cgaaggcguc gaacccgacg acguggacug cuggugcaac      300

accaccucca ccugggucgu guacgggacc ugccaccaca agaaaggaga agcccgacgc      360

ucaagacgcg cgguaacacu accaucacac ucaacccgaa aacugcaaac ccgcagucag      420

accuggcucg agucgcgaga auacacaaaa caccugauac gaguagaaaa uuggaucuuc      480

cgaaacccgg gauucgcccu cgccgccgcc gcaaucgccu ggcuccuagg auccuccaca      540

agccaaaaag ucaucuaccu cguaaugauc cuccuaaucg caccagccua cagcauaaga      600

ugcauaggag uaagcaauag agacuucguc gaaggaaugu caggcggcac auggguagac      660

gucguacuag agcacggagg augcgucacc gcaauggccc aagacaaacc aacaguagac      720

aucgaauuag ucacaacaac aguguccaac auggcagaag uacgcucaua cugcuacgaa      780

gccagcauca gcgacauggc gagcgacucc cggugcccca cgcaaggcga agccuaucuc      840

gacaaacagu cagacaccca auacgucugc aaacgaacuu uaguugaccg cggcuggggc      900

aacggaugcg gccucuucgg caaaggauca cucgucacgu gcgcgaaauu cgccugcagc      960

aagaaaauga cagggaaauc aauucagccu gaaaaccuag aauaccgcau cauguuaagc     1020

guccacggau cacagcacuc cggcaugcuc gugaaugaua caggccacga aaccgacgaa     1080

aauagagcga aagucgaaau caccccgaac ucuccacggg cagaagcaac acuaggcggc     1140

uucggcuccc ucggacucga uugcgagccc cgcaccggac ucgacuuuag ugaccucuac     1200

uaccucacaa ugaacaacaa acacuggcuc gcacacaaag aaugguucca cgacaucccg     1260

cuacccuggc acgccggagc agccaccggc acaccccauu ggaauaacaa agaagcacuc     1320

gucgaguuca aagacgcaca cgcgaaacgc caaacagucg uagucuuagg aagucaggaa     1380

ggcgccgucc acaccgcucu cgccggcgcc cucgaagccg aaauggacgg cgccaaaggc     1440

cgacucucca gcgggcaccu aaaaugccga cucaaaaugg acaaacucag acucaaaggc     1500

guaucauaca gccucugcac agcagcauuc accuucacaa aaauccccgc ugaaaccguu     1560

gacggcacag uaaccguaga aggccaauac gggggcaccg acggccccug caaagucccc     1620

gcgcaaaugg cgguagacau gcaaacccuc acgcccguag gccgccucau aacagccaac     1680

cccgugauca cagaaucaac ggaaaacagc aaaaugaugc uagaacugga ucccccauuc     1740

ggagacucau acauagugau cggugucgga gaaaagaaaa ucacccacca cuggcaccgc     1800

ucaggcucaa caauuggaaa agccuucgaa gcaacaguca gaggcgcaaa acgcauggcc     1860

guccugggag acacagccug ggacuucggc ucagugggcg gcgcccucaa cucccucggg     1920

aaaggcaucc accagaucau cggagcggcc uucaaaagcc uauucggcgg aaugucaugg     1980

uucagccaaa uacuaauagg aacacuacua auguggcuag gacucaacac caaaaacggc     2040

aguaucucac ucaugugccu agcccucggc ggagucuuaa ucuuccucuc aacagcugua     2100

ucaggcgggg uaggcugcuc cguagacuuc uccaag                               2136


<210>  2892
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long CDS opt

<400>  2892
cgaaucauaa acgcacgcaa agaaaagaaa cgacgaggga cggacaccuc cgucggaauc       60

guaggccucc uucugaccac cgcaauggcc gccgaaguca cccgccgagg cucugcauac      120

uacauguacc ucgaccgcag cgaugccgga gaagcgaucu cauuccccac aacacucggc      180

augaacaaau gcuacauuca aaucauggac cucggacaca ugugcgacgc cacaaugucc      240

uacgaaugcc ccaugcuaga ugaaggcguc gaaccagacg augucgacug cuggugcaau      300

acaaccagca ccugggucgu guacggcaca ugccaccaca agaaagggga agccagacgc      360

agccgacgag cagucacacu accgucacac agcacacgua aacuccaaac ccgcagccaa      420

acauggcugg aguccagaga guacaccaaa caccugauaa ggguagaaaa cuggauauuc      480

cgcaacccug gcuucgcccu cgccgccgca gcaaucgccu ggcuacucgg cucaucgacc      540

ucccaaaaag uaaucuaccu cgucaugaua cuucucaucg cccccgcaua cuccauccga      600

ugcauaggag uguccaaccg cgacuucgug gaaggcaugu ccggcggaac cugggucgac      660

guaguacuag aacacggagg augcguaacg gucauggcac aagacaaacc cacaguugau      720

auagagcucg uaaccaccac cgucagcaac auggccgaag uacgcucaua cugcuacgaa      780

gccuccaucu cggacauggc uagcgauucc cgcugcccca cacaaggaga agcguaccug      840

gacaaacaau cggacacaca auacguaugc aaacggaccc uagucgaccg cgguuggggc      900

aacggcugcg gacuuuucgg aaaaggaagc cucguaacau gcgccaaauu cgcaugcucg      960

aaaaagauga ccggcaaaag cauacaacca gagaaccucg aguacagaau aaugcucuca     1020

guccacggcu cccagcacuc cggcaugauu gugaaugaca cgggccacga aaccgacgag     1080

aaucgcgcua aggucgaaau cacacccaau ucaccacgcg cggaggccac acucggcggg     1140

uuuggaagcc ucggccucga cugcgaaccc agaacaggac uagacuucag cgaccuguac     1200

uaccucacca ugaacaauaa acacuggcua guccacaaag aaugguucca cgacaucccc     1260

uuaccauggc augcaggcgc cgacaccggc acaccccacu ggaacaacaa agaagcccuc     1320

guagaguuca aagacgcaca cgccaaacga caaacaguag ugguacuggg aucucaggaa     1380

ggagccguac acacagcccu agcaggcgca uuagaagccg aaauggacgg agccaaaggu     1440

cgccucucua gcggacaucu caagugccgc cugaaaaugg acaaacucag acuaaaggga     1500

gucucuuacu cauuaugcac cgcagccuuc acauuuucca agaucccagc cgaaacacug     1560

cacggcaccg ucaccgagga aguacaguac gccggaaccg acggcccaug caagguacca     1620

gcccaaaugg cggucgacau gcaaacacua acccccgucg gccgacucau uacagcuaac     1680

cccguuauca cagaaucaac cgaaaacucg aaaaugaugc uagaacuaga cccacccuuc     1740

ggagacuccu acaucgucau cggcguaggc gaaaagaaaa uuacacacca cuggcaccgc     1800

aguggcagca cgauaggaaa agcauucgaa gcaacugucc gcggagccaa acgcauggca     1860

guccucgggg acaccgcaug ggacuucgga agcgucggag gcgcccucaa cucgcucgga     1920

aaaggaaucc accaaauuuu cggagcagcg uucaaguccc uguucggggg caugagcugg     1980

uucagccaaa uacucauagg aacacuacuc augugguuag gcuuaaacac uaagaauggc     2040

agcaucagcc ucaugugccu cgcccuaggc ggaguacuca ucuuccucag cacagccgug     2100

agcgccgacg uaggcugcag cgucgacuuc ucaaaa                               2136


<210>  2893
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long CDS opt

<400>  2893
cgcauaauca acgcccggaa agaaaagaaa cgacgaggca cugacaccuc cgucggaaua       60

gucggacuac uacuaacuac agccauggcc gcagaaguca caaggcgcgg aucagcauac      120

uacauguacc uagaccgcuc agacgccggc gaagccauca gcuucccaac cacacuagga      180

augaacaaau gcuacauaca gauaauggac cugggacaca ugugcgacgc cacaaugagc      240

uacgaaugcc caaugcuaga cgaaggcguu gaacccgacg acguagacug cugguguaac      300

acgaccagca ccugggucgu auacggaaca ugccaccaca agaaaggcga agcuaggcgc      360

ucccgacgcg ccgucacccu accgagccau uccaccagaa aacuccaaac gaggucccag      420

accuggcugg aaucccgaga auacacaaaa caccuuauac gaguagaaaa cuggauauuc      480

cgaaacccgg gguucgcccu ggccgccgcu gccauagccu ggcuccucgg cuccuccaca      540

agccaaaaag uaauauaccu gguaaugauc cuacuaauag cccccgccua cagcaucagg      600

ugcaucggcg ucucaaacag agacuucguc gaaggcaugu ccggcggaac cuggguggac      660

guaguccugg agcauggcgg augcgugaca guaauggccc aagacaagcc aaccguagac      720

aucgaacugg uaacuacaac cgucagcaac auggccgaag uccgaagcua cugcuacgaa      780

gccagcauaa gcgacauggc cuccgacucc cggugcccaa cacaaggcga agccuaccua      840

gacaaacaau ccgacacgca auacguaugc aaacgaaccc uagucgacag aggauggggc      900

aacggcugcg gccucuucgg caaaggauca cucgucacgu gcgccaaguu cgccugcagu      960

aagaaaauga caggcaaauc gauacaaccc gaaaaccuag aauaccgcau caugcuaagc     1020

guacacggca gccaacacuc aggaaugauc gugaacgaca caggccacga aacagacgaa     1080

aaucgagcaa aaguagaaau caccccuaac agcccacgug ccgaagcaac acuaggagga     1140

uucggcagcc uaggccuuga cugugagcca cgaaccggac ucgacuucuc cgaccuguac     1200

uaccucacga ugaauaacaa acauugguua guccacaaag aaugguucca cgacauacca     1260

uugccauggc acgcaggcgc ggauaccgga acgccacacu ggaacaacaa agaagcacuc     1320

gucgaguuca aagaugcaca cgcaaaaaga cagaccguag ucguacuugg cagucaagaa     1380

ggcgccgucc acaccgcgcu ugccggagcc cuagaagccg aaauggacgg cgccaaaggc     1440

cgacucuccu ccggacaccu caaaugccga cugaaaaugg acaaacuccg acucaaaggc     1500

guaagcuaca gcuugugcac cgccgccuuc acauucacaa aaauaccagc agaaacucuc     1560

cacggcaccg uaaccguaga aguacaguac gccgggacag acggccccug caagguacca     1620

gcacaaaugg ccgucgacau gcaaacccua acacccguug gcagacuaau cacagcgaac     1680

ccaguaauca ccgaaagcac cgaaaauagc aagaugaugc ucgaacucga ucccccauuc     1740

ggcgacagcu acauagucau aggagucggc gagaagaaaa ucacccacca cuggcaccga     1800

agcggcagca ccauaggaaa agccuucgaa gccacaguga gaggcgcgaa aagaauggca     1860

guccucggcg acacagcgug ggacuucggc agcgucggcg gcgcacucaa cucacucggc     1920

aaaggcaucc accaaaucuu cggcgcagcc uucaaaagcc ucuucggcgg cauguccugg     1980

uucagccaaa uacuaaucgg gacccuacug auguggcuag gacuaaacac caaaaacgga     2040

ucgaucuccc ucaugugcuu agcacucggc ggcguccuaa uauucuuaag caccgccgua     2100

uccgcagacg ugggcugcag ugucgacuuc ucaaaa                               2136


<210>  2894
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long CDS opt

<400>  2894
cgaauaauca acgcacgcaa agagaaaaag agaagaggaa cagacacauc agucggcauc       60

gucggauuac ugcucacgac ggcaauggcg guagagguga cccgcagggg aaacgccuac      120

uacauguacc uagacagaag cgacgcagga gaggcuaucu ccuucccgac cacaaugggc      180

augaacaaau gcuacaucca aaucauggac cuagggcaua ugugcgacgc aaccaugagc      240

uacgaaugcc caauguuaga cgagggaguc gaaccagacg acguugacug uuggugcaau      300

accacaagca ccugggucgu auacggcaca ugccaccaca agaaagggga agcgaggcgc      360

agucgacgcg ccgucacccu acccagccau uccacacgca aguuacaaac gcgcagccaa      420

acguggcuag aaagccgaga auacaccaaa cacuuaauca gagucgaaaa cuggaucuuc      480

agaaacccgg gauucgcacu agcagccgcc gcgaucgccu ggcuacuagg uucgagcaca      540

agccaaaaag ucaucuaccu agugaugaua cuccuaauag cccccgcaua cagcauacgg      600

uguauaggcg uaucaaacag agauuucguc gagggaaugu ccggcggaac auggguagac      660

guaguacugg aacacggcgg augcguaaca gucauggcac aggacaaacc cacagucgac      720

auagaacucg ucaccaccac cgucagcaac auggccgaag uacgcagcua cugcuacgag      780

gcaucaauau ccgacauggc auccgacagc agaugcccaa cgcagggaga ggccuaccua      840

gacaaacaau ccgacacaca guacgugugc aaaagaaccc uaguagaccg agguuggggc      900

aacggcugcg gacuauucgg gaaaggcucg cuaguaaccu gcgccaaauu cgccugcagc      960

aagaaaauga ccggcaaauc aauucaacca gaaaaccucg aauaccgaau caugcucagc     1020

guccacggaa gccaacacag cggaaugaua guaaacgaca ccggccacga aacagacgag     1080

aacagagcga aagucgaaau aacacccaac aguccccgcg cagaagccac ccugggagga     1140

uucggcagcc ucggccuaga cugcgaacca cgaacaggac uagacuucag cgaccuguac     1200

uaccugacaa ugaacaacaa acauuggcua guacacaaag aaugguucca cgacauacca     1260

cucccuuggc acgcuggcgc cgacacagga acaccccacu ggaacaacaa agaagcccua     1320

gucgaguuca aagaugcaca cgcaaaacgc caaaccguag ugguccuggg cucacaggaa     1380

ggagccgugc acacagccuu agcgggcgcg cucgaagccg aaauggacgg agccaaaggc     1440

agauuaagca gcggacaccu caaaugccgg cugaaaaugg acaaacugcg acucaaagga     1500

guauccuaca gccucuguac agcggccuuu acauucacaa aaauaccagc ggaaacgcuu     1560

cacgggacag uaacaguuga aguacaauac gcuggaacag acggacccug caaaguaccg     1620

gcacagaugg ccgucgacau gcaaacccua accccaguag gacgccugau aacggccaac     1680

ccagugauca ccgaaagcac cgaaaacuca aaaaugaugc ucgaacucga cccgccauuc     1740

ggcgacagcu acauagucau aggaguggga gaaaagaaaa uaacgcacca cuggcaccgc     1800

agcggcagua caaucggaaa agccuucgaa gcaaccgugc gcggcgcaaa aagaauggcc     1860

guacuaggag acaccgcuug ggacuucggc agcguaggcg gagcccucaa cagccucggg     1920

aaggggaucc accaaaucuu cggagcagcc uucaagagcc uuuucggagg cauguccugg     1980

uucagccaga uacuaauagg gacccuacug guauggcuag gucucaacac caaaaacggc     2040

agcauaaguc uuaugugccu cgcacuaggc ggaguacuaa uauuccuaag cacagcagug     2100

agugccgacg uaggcugcag cguagacuuc uccaag                               2136


<210>  2895
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long CDS opt

<400>  2895
cgaaucauaa acgcccgcaa agaaaagaaa aggagaggca cagacacaag cguaggaauc       60

guggggcucc ugcuaacaac agccauggcg gccgagguca cccguagagg cagcgcguac      120

uacauguacc uagaccgcag cgacgccgga gaggccauca gcuucccgac aacccuaggc      180

augaacaaau gcuacauaca aauaauggac cucggccaca ugugcgacgc gaccauguca      240

uacgaaugcc caaugcugga cgaaggaguc gaacccgacg acguagacug cuggugcaac      300

acaacgagca cguggguagu cuacggaacc ugccaccaca agaaaggcga agcacgacga      360

ucccgacgag cggucacgcu cccaagccac uccacccgaa aacuccagac cagaagccaa      420

accuggcuag aauccaggga auacaccaaa caccucaucc gcguagaaaa cuggauauuc      480

cgaaaccccg gcuucgcacu cgcagcagca gccauagcau ggcuacucgg cagcagcaca      540

agccaaaaag uaauauaccu ggugaugaua cuacugaucg cccccgccua cagcauccgc      600

ugcauuggag ucaguaacag agacuucgua gagggcauga guggaggaac auggguggac      660

gucguccugg aacacggagg augcgucaca guaauggcac aagacaaacc cacagucgac      720

aucgaacucg uuacaacgac cgucucaaac auggccgaag uacgcagcua cugcuacgag      780

gcaaguauaa gcgacauggc aagcgacagc cgaugcccga cccaaggaga agcauaccug      840

gauaaacaaa gcgacacgca auacgucugc aaaagaaccc uagucgaccg cggauggggg      900

aacggaugcg gacuauucgg aaaagggagc cucguaacau gcgcaaaauu cgcgugcuca      960

aagaaaauga caggcaaaag cauccaacca gaaaaccuag aauaccgaau caugcuaagc     1020

guacacggau cccaacacag cggaaugaua gucaaugaca cgggacauga aacagacgaa     1080

aacagagcca aagucgaaau aacaccaaac aguccccgcg cggaagccac ccucggggga     1140

uucggaagcc ucggcuuaga cugcgagccg cgcacaggac uagacuucag cgaccuauac     1200

uacuuaacca ugaauaacaa acauuggcuc guccacaaag agugguucca cgacauccca     1260

cuuccguggc acgccggcgc ggacacaggc acaccgcacu ggaacaacaa agaagcccuc     1320

guggaguuca aagacgccca cgccaaacga caaacagucg uaguucuagg gucccaagaa     1380

ggcgcaguac acaccgcacu cgcaggcgcc cucgaggccg aaauggacgg cgccaaaggc     1440

cgacugucca gcggacaccu caagugcaga cuaaaaaugg acaagcuccg ccuaaaaggc     1500

guaucauacu cgcuauguac agcggcauuc acauucacca aaaucccagc cgaaacccuc     1560

cacggaaccg uaacaguaga aguacaauac gcaggaacag acggcccgug caaaguaccg     1620

gcccaaaugg caguagacau gcaaacacuc acacccgucg gaaggcuaau cacagcaaac     1680

ccagucauaa cggaaagcac agaaaacagc aaaaugaugc ucgaacuaga cccaccguuc     1740

ggcgacagcu acauaguuau agggguagga gaaaagaaaa uaacacacca uuggcauaga     1800

uccggcagca caaucgggaa agcauucgaa gcgacaguca gaggagcuaa acgaauggcc     1860

guacucggcg acacagccug ggacuucgga uccgugggcg gggcccugaa cagucucggc     1920

aaaggaaucc accaaaucuu cggagccgca uucaaaucgc uauucggcgg cauguccugg     1980

uucucccaga uccuaaucgg aacccuauua auguggcucg ggcuaaacac caaaaacgga     2040

uccauuuccc ugguaugccu cgcccucgga ggaguacuca uauuccugag cacagccguc     2100

agcgccgaug uaggaugcuc cguggacuuc agcaaa                               2136


<210>  2896
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long CDS opt

<400>  2896
cgcauaauca acgcaagaaa agaaaagaaa cgccgaggca cagacaccag cgugggcaua       60

gucggacuac uccuaacaac cgccauggcc guugaaguca cacgacgagg aagcgcauac      120

uacauguacc uagaccguag cgacgccgga gaagcaauau ccuucccaac cacccuagga      180

augaacaaau gcuacaucca aauaauggac cuaggccaua ugugcgacgc uacgaugagu      240

uacgaaugcc ccaugcugga cgaaggcgua gagccggacg acgucgacug cuggugcaac      300

accaccagca ccuggguagu cuacggaacc ugccaccaca agaaaggcga ggcacgcaga      360

agccgccgag cagucacccu accaucccac ucaacacgca aacuacaaac aagaucccaa      420

accuggcugg aaagccgcga auacacaaaa caccugauac gagucgagaa cuggaucuuc      480

cgaaacccag gcuucgcccu cgccgcagcc gccaucgcgu ggcuacucgg cagcuccacu      540

agccaaaaag ugauauacuu aguaaugaua cuccucaucg ccccagccua cucgauccgc      600

ugcaucggcg ucagcaauag ggauuuugua gaaggcauga gcggcgguac uugggucgac      660

gucgugcuag agcacggcgg cugcgucaca guaauggcac aagacaaacc cgccgucgac      720

aucgaacucg uaacgacgac aguaucaaac auggccgaag ucagaucgua uugcuacgaa      780

gccuccauca gcgacauggc cagcgacucc cgcugcccaa cacagggaga agcauacuua      840

gacaaacaaa gcgacacaca auacguaugc aaacgaaccc uagucgaccg cggauggggg      900

aacgggugcg gucuauucgg aaaaggauca cucguaaccu gcgccaaauu cgcgugcagc      960

aagaaaauga ccggcaaauc gauacaacca gaaaaccucg aauacagaau caugcuaucc     1020

guccacggcu cccaacacuc uggcaugauc guaaacgaua ccggccacga aaccgacgaa     1080

aaccgagcca aaguggaaau caccccgaac agcccucggg cagaagccac gcugggcggg     1140

uucgggagcc uaggacucga cugugaacca cgcaccggac uagauuucag cgaccuauac     1200

uaccugacca ugaacaacaa acacugguua guccacaaag aaugguucca cgacauacca     1260

cucccauggc acgcaggcgc cgacacaggc accccacacu ggaacaacaa agaagcacug     1320

guagaguuca aagacgccca cgccaaacgc caaacggucg uagugcuagg gucacaggaa     1380

ggcgcggucc acaccgcccu cgccggcgcc cuggaagcag aaauggacgg ugccaaagga     1440

cgccucagcu caggacaccu aaaaugccga cucaaaaugg acaaacugcg ccucaaaggg     1500

gucucauacu cacuaugcac cgccgccuuc accuucacca aaaucccagc cgaaacacuc     1560

cacggaacag uaaccgugga aguccaguac gccggcaccg acggacccug caaggugcca     1620

gcacaaaugg ccguagacau gcaaacccuc accccagucg gacgccuaau aacugccaac     1680

cccgucauca ccgaaagcac agaaaacuca aaaaugaugc uugaacugga ucccccauuu     1740

ggcgacagcu acaucgucau cgggguuggc gaaaagaaaa ucacacacca cuggcaucgc     1800

ucaggcucca ccaucggcaa agcguucgaa gccaccgucc gcggcgcaaa acgcauggcc     1860

guacugggag acaccgcaug ggacuuugga agugucggag gcgcccugaa cucacuaggg     1920

aaaggaauac accaaaucuu cggagcagcg uucaaaagcc ucuucggcgg aaugaguugg     1980

uucagccaaa uccuuauagg aacccuccuc guauggcucg gguuaaacac caaaaacggg     2040

agcauuagcc uuacaugccu agcccucggc ggcguacuca uauuccucag caccgcuguc     2100

ucggccgacg ucggcuguuc cguagacuuc agcaaa                               2136


<210>  2897
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long CDS opt

<400>  2897
cggaucauca acgccagaaa agaaaagaaa cggcgcggag cggacacauc agucgggauc       60

guaggccuac uucucaccac ggcaauggca gucgaaguaa caagacgcgg aagcgccuac      120

uauauguacc ucgaccgcuc cgacgccgga gaagcaauca gcuuccccac uacccuaggc      180

augaacaaau gcuacaucca aaucauggac cucggacaca ugugugacgc cacgauguca      240

uacgaaugcc ccaugcucga cgaaggagug gaacccgacg acguagacug cuggugcaac      300

accacaucaa cauggguugu cuacggaacc ugccaccaca agaaaggaga agcccgucga      360

agccggcgag ccguaacacu cccuucgcac ucaacccgaa aacugcaaac aagaucccaa      420

acauggcuag aaagucguga auacacaaaa caucuaauac guguggagaa cuggaucuuc      480

cgaaaccccg gauucgcauu agcagccgcc gucauagccu ggcugcuagg aagcagcacc      540

agccaaaaag uaauauaccu ggucaugauc cuacucauag cgccagccua cuccaucaga      600

uguauaggcg ucucaaaccg ggacuucguc gagggaaugu ccggaggcac uugggucgac      660

gugguacugg aacacggcgg augcgucaca gucauggcac aagacaaacc aacuguagac      720

auagaacucg ucacgaccac ggucagcaac auggccgagg ucagaagcua cugcuacgaa      780

gccagcauuu cagacauggc aagcgacagc cggugcccga cccaaggaga agcguaccua      840

gacaagcaaa gcgacaccca auacgucugc aaaagaaccc ucgucgaccg gggcuggggc      900

aacggcugcg gacucuucgg caaaggaagu cuaguaacuu gcgcaaaauu ugcgugcucg      960

aagaaaauga ccggcaaauc aauccaaccc gagaaccuag aguacagaau aaugcuauca     1020

guucacggca gucagcacuc cggaaugauc guaaacgaca ccggccacga aaccgacgaa     1080

aaccgcgcaa aagucgaaau aacacccaac agcccccgag ccgaagccac acucggaggg     1140

uucggcuccc uaggacuaga cugcgagcca cgcaccggac ucgacuucuc agaccuauac     1200

uaccuaacca ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauaccg     1260

cuccccuggc acgccggagc ggacaccggc acaccccacu ggaacaacaa agaagcccua     1320

gucgaguuca aagacgcaca ugcaaaacgc caaaccguag ucguacucgg cagccaggaa     1380

ggggccgugc acacagcccu agcaggagcc cucgaagccg aaauggacgg agccaaagga     1440

cgacucucaa gcggccaccu caaaugccga cuaaaaaugg acaaacuacg acuaaaaggc     1500

gucagcuaua gccugugcac cgcagccuuu accuuuacua agauucccgc ggaaacccua     1560

cauggcaccg ucaccguaga gguacaauac gccggcaccg acggcccaug caaagucccc     1620

gcccaaaugg ccgucgacau gcaaacgcuc acaccagucg gaagacuaau aacagccaac     1680

cccgucauca ccgaaaguac cgaaaacagu aaaaugaugc uagaacucga uccacccuuc     1740

ggagacucau acaucgucau cggagucggc gaaaagaaaa uaacacacca uuggcauagg     1800

ucgggcucca caauaggaaa agcauucgaa gccaccgucc gcggugcaaa acgaauggca     1860

guccuagggg acacagcaug ggacuucggc agcgucggag gugcacuaaa cucccucggc     1920

aaaggcaucc accaaaucuu cggcgcagcc uucaaauccc uguucggagg cauguccugg     1980

uucagccaaa uccucaucgg cacccuauua guauggcuag gccucaacac gaaaaauggc     2040

ucgauaucac uaacaugucu cgcccuaggc ggaguccuca ucuuccuaag caccgccgua     2100

uccgcagacg ucggaugcuc cguagacuuc ucaaaa                               2136


<210>  2898
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long CDS opt

<400>  2898
cgaauuauaa acgcccgcaa agagaagaaa agacgcggag ccgacacauc cguggguauc       60

gucggacucc uccuaaccac cgccauggcc gucgaaguaa cccgacgcgg auccgcguac      120

uacauguacc uugaccgcuc agacgcgggg gaagccauau cuuuucccac cacacuagga      180

gucaacaaau gcuacaucca aaucauggac cuaggccaca ugugcgacgc cacaaugagc      240

uacgagugcc ccaugcucga cgaagggguu gaaccagacg acguugacug cuggugcaac      300

accacgucca ccuggguugu cuauggaacc ugccaccaca agaaaggcga agcccgccgg      360

ucccgccgag ccgucacccu gcccagccac agcacacgca aacuacaaac ccgaucucaa      420

acauggcuag agucacgaga auauacaaaa caccucaucc gagucgaaaa uuggaucuuc      480

cgcaacccag gcuucgcgcu cgccgcagcc gccaucgcau ggcuccucgg auccucaacc      540

ucacaaaaag uuauauaccu gguaaugaua uuacucauag caccagccua cucaauacga      600

ugcauaggag ucuccaaccg cgacuucguc gagggcauga gcggaggcac cuggguagac      660

gucguccuag aacacggagg cuguguuacc gucauggcgc aagacaaacc cacagucgac      720

aucgaacucg uaacaaccac cgucuccaau auggcggagg uacgaucaua uuguuacgaa      780

gcaucaauau cagacauggc gagcgacagu cgcugcccga cccaaggcga agccuaucuu      840

gacaaacaaa gcgacacaca guacguaugc aaacgaacac ucguagaccg aggcuggggc      900

aacggcugug gccuguuugg caaagguagc cugguaacau gcgcaaaauu cgccugcucc      960

aagaaaauga ccggcaaauc aauacaaccc gaaaaccuag aauaccgcau caugcugucc     1020

guccacggca gccaacacag cggcaugauc gucaacgaca ccggacacga aaccgacgaa     1080

aaccgcgcca aaguagagau aacaccuaac ucaccccgag ccgaagccac acucggaggc     1140

uuuggcagcc uaggacucga cugcgaaccc cgaacaggac ucgacuucuc cgaccuauau     1200

uaccuaacaa ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauacca     1260

cuacccuggc acgccggagc cgacaccgga acaccccacu ggaacaacaa agaagcacuc     1320

gucgaguuca aggacgcaca cgccaaacgg cagaccgucg uuguccuagg cucacaagaa     1380

ggcgcggucc acaccgcucu cgcaggcgcc cucgaagcag aaauggacgg cgccaagggg     1440

aggcucuccu ccggacaccu caagugccgc cuaaaaaugg acaaacuacg gcucaaagga     1500

guaagcuaca gccucugcac agcagcauuc acauucacca aaauaccagc agaaacgcua     1560

cauggaacag ucacggucga aguacaauac gcgggcacag acggacccug caaagugccc     1620

gcacaaaugg cgguugacau gcaaacccuu acgccagucg gccgacucau caccgccaac     1680

cccgucauua cagaauccac agaaaacucc aaaaugaugc uugaacugga cccacccuuc     1740

ggcgacucgu acauaguaau aggcgucggc gacaagaaaa ucacccacca cugguaccgc     1800

uccggcucaa caaucggaaa agcauucgaa gcaaccgucc gaggugccaa acguauggca     1860

guacuaggcg acaccgccug ggacuucggc uccguaggag gagcacucaa cucccucgga     1920

aaaggaaucc aucaaauauu cggagccgcc uucaaguccc uauucggcgg caugucaugg     1980

uuuagccaga uccucauagg aacccugcua guauggcuag gccucaacac caaaaacggg     2040

ucaaucucuu uaaccugccu agcauuaggg ggaguucuca uauuccucag cacagcagua     2100

agcgccgacg uaggcuguuc cgucgauuuc ucaaaa                               2136


<210>  2899
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long CDS opt

<400>  2899
cgcauaauca acgcacgcaa agaaaagaaa agacgcggag cagacacaag cgugggaauu       60

guaggccugc uauuaaccac ugccauggcg guagaaguaa cacgacgcgg aucagcauac      120

uacauguacc uggacagaag cgacgcuggc aaagccaucu ccuucccaac aacgcuggga      180

guaaacaaau gcuacaucca aauaauggac cuagggcaca ugugcgacgc cacaaugagc      240

uacgaaugcc cgaugcucga cgagggagua gaaccagacg acgucgacug cuggugcaac      300

acaacuagca cauggguagu auacggcacc ugucaccaca agaaaggaga agcacgccgg      360

agccgacggg cagucacgcu acccucacac agcacacgaa aacuacaaac ucgcucccaa      420

accuggcucg aaagccgaga auacaccaaa caccuaaucc gcgucgagaa cuggaucuuc      480

cgcaacccag gauucgcacu agcagcugca gcaaucgcgu ggcugcucgg aagcagcaca      540

agccaaaaag uaauauaccu aguaaugaua cuccucaucg cccccgccua uaguauacgg      600

ugcaucggcg ucagcaaccg agacuucguc gaaggcauga gcggcggcac gugggucgac      660

gucguccucg aacauggcgg augcgucaca guaauggccc aggacaaacc aaccgucgac      720

aucgaacuug ucacaacgac cgugagcaac auggccgaag uccgcuccua cugcuaugaa      780

gccuccaucu cagauauggc cucagacagc cgaugcccaa cccagggcga ggcauacuug      840

gacaaacaaa gcgacaccca auacgucugc aagcgaacac uggucgaccg aggguggggu      900

aauggaugcg gccuauucgg caagggaagc cuagucaccu gugcaaaguu cgccugcuca      960

aagaaaauga cagggaaauc cauccaaccc gaaaaccucg aguaccgcau aauguugagc     1020

guccacggcu cccaacacag cggcaugaua guaaacgaca ucggccauga aaccgacgaa     1080

aaucgagcaa aaguagaaau aacaccuaac aguccgagag cggaagcaac acuaggaggc     1140

uucggcucgc uaggccuaga cugcgaaccc cgaaccggac ucgacuucag cgaccuauac     1200

uaucucacua ugaacaacaa acacuggcuc gugcacaaag agugguucca cgacaucccg     1260

cuccccuggc acgcaggagc cgacacaggc accccacacu ggaacaacaa agaagcgcua     1320

gucgaguuca aagacgcaca cgcuaaaaga caaaccgugg ucguccuagg cucacaagaa     1380

ggcgcaguac acacagcacu agccggcgcu cucgaagcag aaauggacgg agccaaaggu     1440

cgacuauccu ccggccaccu aaaaugccgc cucaaaaugg auaaacuccg ccucaaaggc     1500

guaucauaca gucuaugcac cgccgcauuc acauucacaa aaauaccagc cgaaacccuc     1560

cacggcaccg uaaccguaga aguacaguac gcaggaaccg acggaccaug caaagugcca     1620

gcccaaaugg cagucgacau gcaaacacuc acuccagucg gacgacuaau caccgcuaac     1680

cccgucauaa ccgagagcac ggaaaauucc aagaugaugc uagaacugga ucccccguuc     1740

ggcgacagcu acauagucau cggagucgga gacaagaaaa ucacacacca cuggcaccga     1800

agcggcagca ccaucggaaa agcguucgaa gcaaccguca gaggcgccaa acgaauggcc     1860

guacucggag acacggccug ggacuucggc uccgucggcg gagcacucaa uucccucgga     1920

aaagguaucc accaaauuuu cggcgccgca uucaaaucac uauuuggggg cauguccugg     1980

uucucgcaga uacuuaucgg cacccuccua guauggcuag gacuaaacac caaaaacggc     2040

agcauaagcc ucacaugccu agcccucgga gggguccuaa uauuccugag cacagccgua     2100

agcgcugacg ucggcugcag cgucgacuuc agcaaa                               2136


<210>  2900
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long CDS opt

<400>  2900
agaaucauca acgcaagaaa agaaagaaaa agaagaggcg ccgacacauc aaucggcauc       60

gucggccugc uacuaacaac agcuauggca gcagaaauca cccgacgagg cucagcguac      120

uacauguacc ucgacagaag cgacgccgga aaagcuauca gcuucgccac gacacuggga      180

gucaacaagu gccacgugca gauaauggau cucggacaca ugugcgacgc aaccaugagu      240

uaugagugcc ccaugcuaga cgaaggcgua gagccagacg acguagacug cuggugcaac      300

acaacaagca ccuggguagu auacggcaca ugccaccaca agaaaggaga agcaagacga      360

agcaggcgcg caguaacacu acccucacac agcacacgaa aacuccaaac cagaagccag      420

acauggcucg agagccguga auacacaaaa caccuaauaa aaguggaaaa cuggauauuc      480

cgcaacccag gguucacccu aguagccgua gcaaucgcgu ggcuucucgg gagcagcacc      540

ucccagaaag ucauauaccu cguaaugauc cuucucauag cuccggccua cagcauacga      600

ugcauaggcg uaagcaaccg cgacuucguc gaaggaaugu cagggggaac auggguggac      660

guaguacuag aacacggagg cugcgucacg guaauggcac aagacaaacc aaccgucgau      720

aucgagcuag ucaccaccac agucagcaac auggcggaag uacgaagcua cugcuacgaa      780

gccucaauca gcgacauggc gagcgacucu cgcugcccaa cacaaggcga agcauaccuc      840

gauaaacaaa gcgacacgca guacgucugc aaacgcaccc ucgucgacag aggguggggc      900

aacgggugug gccuauuugg aaagggauca uuagucacau gcgcaaaauu cacaugcagc      960

aagaaaauga cagggaaaag cauccaaccu gagaaccucg aauaccgcau aaugcucagc     1020

guccauggau cacaacacuc cggcaugauc gucaacgaca cgggacacga aacagaugaa     1080

aaucgagcga aaguagaggu cacaccgaau agccccagag ccgaagccac acuaggagga     1140

uucggaagcc ucggccugga cugcgaaccg cgaaccggcc ucgacuucag cgaccuauac     1200

uaccucacca ugaacaacaa acauuggcua guccacaaag aaugguucca cgacaucccc     1260

cuccccuggc acgcaggcgc cgacacggga accccucacu ggaacaacaa agaagcacug     1320

guugaguuca aagacgcaca cgccaaaaga caaacaguag uaguauuagg gagccaggaa     1380

ggagccguac acacagcccu cgcaggcgcc cuagaagccg aaauggacgg agccaaaggc     1440

agacuguuca gugggcaccu caaaugcagg cuaaaaaugg acaaacuacg ccuaaaaggc     1500

guaagcuaca gccuaugcac agccgcauuc accuucacaa agguccccgc ggaaacacug     1560

caugggacgg uaacaguaga agugcaauac gcaggcacag auggacccug caaaguacca     1620

gcacaaaugg ccguagacau gcaaacacua acacccgucg gaagacucau caccgcaaac     1680

ccagucauca cggaaucaac agaaaacagc aaaaugaugu uagaacugga cccacccuuc     1740

ggcgacucau acauaguaau aggcgucgga gacaagaaaa ucacgcauca cuggcaccgc     1800

agcggcagca caauuggcaa ggcauucgaa gcaacaguca gaggagcgaa acgaauggca     1860

guacucggcg auacagccug ggacuuugga uccgugggag gcguguucaa cagccucgga     1920

aaagguaucc accaaaucuu cggcgcagcg uucaaaucac uauucggcgg caugagcugg     1980

uucagccaaa uacucaucgg aacacuccua guauggcuag gacucaacac caaaaacggc     2040

agcaucagcc ugacaugccu agcgcucgga ggagucauga uauuccucuc aaccgcagug     2100

ucggccgacg uagggugcag ugucgacuuc agcaaa                               2136


<210>  2901
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long CDS opt

<400>  2901
agaauaauca acgcccgcaa agagcgcaaa agaaggggag cagacacgag cauaggcauc       60

aucggacucc ugcucaccac cgcaauggca gcagaaauca ccagaagagg aucagccuac      120

uacauguacc uggacagaag cgacgcggga aaagcaauau ccuucgcaac gaaucuaggc      180

gucaacaaau gccacguaca gaucauggac cuaggucaua ugugcgacgc caccauguca      240

uacgaaugcc caaugcuaga cgaaggcguc gaaccagacg acgucgacug cuggugcaac      300

accacgucca ccuggguggu auacggcaca ugccaccaca agaaaggcga agcccgaagg      360

agcagacgcg cugucacccu accaagccac aguacgcgca agcuccaaac aagaagccag      420

accuggcuag aaagccgcga auacacaaag caccuaauca aagucgaaaa cuggaucuuc      480

cgaaacccag gauucgcccu cgcagccguc gcaaucgcau ggcugcuagg aagcuccacc      540

agccaaaagg uaaucuaccu ggucaugauc cuacugaucg cacccgccua cucgauucga      600

ugcaucggcg uaucgaaccg cgacuucguc gaagguauga gcggcggaac cugggucgac      660

gucguacuag aacauggcgg cugcguuaca guaauggcac aagauaagcc caccguagac      720

aucgaacucg ucaccacgac cguaagcaac auggcagaag uccgcucuua cugcuacgaa      780

gcaagcauca gcgacauggc aagcgacuca cgauguccaa cccaagggga agcauaccuc      840

gacaagcaaa gcgauacaca auacguaugc aaaaggaccc ucgucgaccg aggcugggga      900

aacggaugcg gacucuucgg caaaggaagc cuaguaacau gcgccaaauu uacaugcagc      960

aagaaaauga caggcaaaag uauccagcca gaaaaccucg aauaccgcau caugcuuagc     1020

guacacggca gccaacacuc aggaaugauc gucaacgaca uaggacacga aaccgacgaa     1080

aacagggcga aaguagaagu cacacccaac uccccacgag cagaagcuac ccugggcgga     1140

uucgggagcc ugggccucga cugugaaccg agaacaggac uagacuucag cgaccucuac     1200

uaccucacaa ugaacaacaa acacuggcug gugcacaaag aaugguucca ugacauaccg     1260

cucccauggc acgcaggagc cgacacagga acaccgcacu ggaacaacaa agaagcccua     1320

gucgaguuca aagacgcaca cgcgaaaagg cagacagucg ucguccuggg aucacaggaa     1380

ggcgcaguac acacagcacu cgccggcgcg uuagaagcag agauggacgg ugccaaagga     1440

cgacuguuuu cagggcaccu caaaugccgg cuaaaaaugg acaaacugcg ccucaaagga     1500

guaucauaca gcuuaugcac cgccgccuuu accuucacca aaguccccgc agaaacccuc     1560

cacggcaccg uaacagugga aguacaauac gcagggaccg acgggccaug caagguaccu     1620

gcacaaaugg ccgucgacau gcaaacacuc acacccgucg gaagacuaau caccgccaac     1680

ccaguaauaa cagaaagcac agaaaacagc aaaaugaugc uagaacuaga cccaccauuu     1740

ggagacagcu acaucgugau cggagugggc gacaagaaaa uaacccauca cuggcaccgc     1800

ucaggcagca caauaggcaa agccuucgaa gcaacaguca gaggagcgaa aagaauggcc     1860

guacugggag acacugccug ggacuucggc uccgucggcg ggguauucaa cagccuuggc     1920

aaaggcgugc accagauauu cggggccgcg uucaaaaguu uauucggggg aaugaguugg     1980

uucucccaaa uacuaauagg cacacuucua guauggcucg gacuaaauac gaaaaacgga     2040

agcauuagcc ucacaugcuu agcccucggc ggaguuauga uauuccucag uacagcagug     2100

ucagccgacg ugggaugcuc cguagacuuc agcaaa                               2136


<210>  2902
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long CDS opt

<400>  2902
cgcaucauaa acgcacgcaa agaaaggaaa cgcagaggag cggacaccuc cauaggaaua       60

auaggccuac uacucacaac cgccauggcg gccgaaauca cgcgacgagg aucugcguac      120

uacauguacc uggaccgcag cgaugccgga aaagccaucu ccuucgcaac gacgcucgga      180

gucaacaagu gccauguaca aauaauggac cucggucaca ugugcgacgc cacgauguca      240

uacgaaugcc cgaugcucga cgaaggcgua gaaccagacg acgucgauug cuggugcaac      300

acaacgagca caugggucgu auacggcacu ugccaccaca aaaagggaga agcacgccgc      360

agccgccgag cagucacacu gccaucccac agcacgcgca aacuacaaac caggagccaa      420

acguggcuag aaagcagaga auacacgaaa caccugauca aagucgaaaa cuggauauuc      480

cgaaaccccg gauucgcccu gguagcagua gcaaucgcau ggcuacuagg cuccagcaca      540

ucccaaaagg ucauauaccu aguaaugaua cuacucaucg caccagccua cagcauaaga      600

ugcaucggcg uaagcaaccg cgacuucguc gaaggaaugu ccggcgggac cuggguugac      660

guaguacucg aacacggcgg augcgucacc gugauggcac aagacaaacc cacgguagac      720

aucgaacucg ucacaacgac agucagcaac auggcggaag ugcguucgua cugcuacgaa      780

gccagcauau cagacauggc auccgacuca agaugcccaa cacaaggcga agccuacuua      840

gacaaacaau cagacaccca auacgucugc aaacggacac uugucgaccg cggaugggga      900

aacggaugcg gacuauucgg aaaaggcucg cuagugacau gcgcaaaguu cacaugcuca      960

aagaaaauga caggcaaauc cauacaacca gaaaaccuug aauaucgaau uaugcucagc     1020

guccacggcu cccaacacuc aggaaugaua gucaacgaca uaggacacga aacagacgaa     1080

aacagagcca aaguggaggu cacaccaaac ucccccagag cagaagcaac acuaggagga     1140

uucggauccc ugggacuaga cugcgaaccc aggacaggcc uugacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauaccc     1260

cugcccuggc augccggggc agacacaggg acaccccacu ggaacaacaa agaggcucug     1320

guagaguuca aagacgcgca cgcgaaaaga caaacagucg ucguccucgg cucccaggaa     1380

ggcgccguac auacagcacu cgcuggcgcg cuagaagccg aaauggacgg cgcaaaaggc     1440

agacucuucu ccggacaccu aaaaugccga cucaaaaugg acaaacuacg acugaaaggc     1500

guauccuaca gccuauguac agcagcauuc accuucacua aaguaccagc cgaaacccua     1560

cacggcacag uaaccguaga aguccaguac gcaggaacag acggccccug caaaguacca     1620

gcacagaugg cagucgacau gcaaacgcug accccgguag ggaggcucau aaccgccaac     1680

ccaguuauca cggaaucaac cgaaaacagc aaaaugaugc ucgaacugga cccacccuuc     1740

ggcgauucau acauaguaau aggaguaggc gacaagaaaa uaacccacca cuggcacaga     1800

uccggcagca ccauaggcaa agccuucgag gccacaguac gcggcgccaa aagaauggcc     1860

guacucggag acaccgcaug ggacuucgga uccgucggag gcguauucaa cagccucggc     1920

aaaggaaucc accaaauauu cggagccgcc uucaaaagcc uauucggagg caugagcugg     1980

uucagccaaa uccuaaucgg gacgcuccua guauggcuag gccucaacac caaaaacgga     2040

agcaucagcc uaaccugucu agcccugggc ggcguaauga ucuuccuaag caccgcgguc     2100

agcgccgacg uaggcugcag cguugacuuu ucaaaa                               2136


<210>  2903
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long CDS opt

<400>  2903
agaauaauca acgcaaggaa ggaaaggaaa aggaggggcg cagacacgag cauagggaua       60

aucggccuac uacugacaac agcgauggcc gccgaaauaa ccaggagggg cagugcauac      120

uacauguacc uggacagaag cgacgccggg aaagcgaucu cguucgcgac uaaccucggc      180

gucaacaagu gccacgugca aauaauggac cucggccaca ugugcgacgc aaccaugucg      240

uacgaaugcc cgaugcuaga cgaaggagug gaacccgacg acguagacug cuggugcaac      300

acaacgagca ccuggguagu auacggaacc ugccaccaca agaaaggcga agcgcgccgg      360

agccggcgag cggucacacu cccaagccau agcacacgaa aacuccagac ccgcagccag      420

acauggcuag aaagcagaga auacacaaag caccugauca aagucgaaaa cuggauauuc      480

agaaacccag gguucgcacu agcagcggua gccaucgccu ggcugcuagg cagcuccacu      540

agccagaagg ucauauaccu cguaaugauc uuacucaucg cgccagcaua cucaauaagg      600

ugcaucggcg ugagcaaccg cgacuucguc gaaggcaugu cgggaggaac cugggucgac      660

gucguccuag aacacggcgg cugcgucaca guuauggcac aggacaagcc cacaguagac      720

auagaacucg ucacaacaac cgugagcaac auggccgaag uacgaagcua cugcuaugaa      780

gcgucaaucu cagacauggc aucagacagc cgaugcccca cacaggggga agcauaccuc      840

gacaagcaaa gcgacaccca auacguaugu aaacgaaccc ucgucgacag aggauggggg      900

aacggaugug gccuauucgg aaaaggcagc cucgugaccu gcgcaaaauu uaccugcuca      960

aagaaaauga cgggaaagag cauccaaccc gaaaacuuag aauacagaau caugcuaucc     1020

guccauggau cccaacacag cggcaugaua gucaacgacg aaaacagagc caaaguggag     1080

gucacgccaa acagcccaag agccgaagcc acccucggcg gauucggcag cuugggccua     1140

gauugcgaac ccaggaccgg acuagacuuc uccgaucuuu auuaccugac caugaacaac     1200

aaacacuggc uaguacacaa agaaugguuc cacgacaucc cacuaccaug gcacgcagga     1260

gcagacacag ggaccccgca cuggaauaau aaagaagccu uggucgaguu caaagacgca     1320

cacgcgaaac gccaaaccgu aguaguacua ggcucccaag aaggugcggu ccacaccgcu     1380

cuagccggag cccucgaagc agaaauggac ggcgccaaag gccgacuguu cucaggccac     1440

cuaaaauguc gacuaaaaau ggacaaauua cgccucaaag gcguaaguua cucgcucugc     1500

acagcggcau ucaccuucac caaagugcca gccgaaacuu uacacggaac cguaaccguc     1560

gaaguacaau augcgggaac agacggaccc ugcaaaguuc cagcucaaau ggcggucgac     1620

augcagacgc ucacccccgu cggccgacuc auaaccgcca accccgucau aacugaaagc     1680

acagaaaacu caaagaugau guuagaauua gacccacccu ucggcgacuc auacaucgua     1740

auaggcguag gagacaagaa aauaacccac cacuggcacc gaucagguag cacgauaggu     1800

aaagcauucg aagccacagu cagaggcgca aaacgcaugg cagugcuggg cgacacagcg     1860

ugggacuucg gauccgucgg cggaguauuc aacucacucg ggaaagggau ccaccaaaua     1920

uucggagccg cauucaaauc ccuauucggc ggaaugagcu gguucuccca aauacuaaua     1980

ggcaccuuau ugguauggcu agggcucaac accaaaaacg gaagcauaag ccugaccugc     2040

cuagcacucg gaggagucau gauauuccuc agcaccgcag ucagcgcaga cgucggaugc     2100

agcguggacu uuagcaag                                                   2118


<210>  2904
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long CDS opt

<400>  2904
agaauuauca acgcccgaaa agaacgcaag cggcgaggcg cagacaccuc aauaggaauc       60

gucggccuac uacuuaccac agcgauggcg gcagaaauca cacgacgcgg cagugcauac      120

uauauguacc uagaccgcuc agacgccggc aaagcaauca guuucgcaac cacccucggc      180

gucaacaagu gccacgucca aauuauggac cuaggccaca ugugcgacgc caccaugucc      240

uacgaaugcc ccaugcuuga cgaaggaguc gaacccgacg acgucgacug uuggugcaac      300

accacaagca ccugggucgu cuacggcaca ugucaccaca agaaaggcga agcgcgaagg      360

agcagacgag cgguaacccu accaucccau uccacccgua aacuucaaac acgcagccaa      420

accuggcucg aaagccgaga auacacaaaa caccuaauca aagucgaaaa uuggauauuc      480

cgcaacccgg gguucgcucu agucgccgug gccauagccu ggcuccuagg cuccucaacg      540

ucacaaaagg uaauauaccu cgucaugaua cuccuaaucg caccagcgua cagcauccga      600

ugcauaggcg ucagcaaccg cgacuucgug gaggggauga gcggaggcac cuggguagau      660

gucguacucg aacauggagg cugcgucaca gucauggcac aagacaagcc aacaguggac      720

auagaacucg ucacaacgac gguuagcaac auggccgagg uacgaagcua cugcuacgag      780

gccagcauau cagacauggc cagcgacuca aggugcccca cccaagggga agcauacuug      840

gacaaacaau ccgacaccca auacgucugc aaacggaccc ugguggaccg aggguggggc      900

aacggcugcg gccucuucgg aaaagguucc cucguuacau gcgccaaauu uacgugcagc      960

aagaaaauga ccggaaaauc aauacaaccu gaaaaccucg aauacagaau aaugcucagc     1020

guccacggcu cgcaacacag cggaaugauc guaaacgaca ccggacacga aaccgacgaa     1080

aaccgcgcca aagucgaagu cacaccaaac uccccacgcg ccgaagccac acucggcgga     1140

uucgguagcc uaggacugga cugcgaacca cgcacaggac uagacuucuc cgaccucuac     1200

uaccuaacca ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauaccc     1260

cucccauggc augcgggcgc cgacaccggc acaccccacu ggaacaacaa agaagcucua     1320

guagaguuca aagacgcaca cgcuaaacgc caaaccgucg uaguccuugg cucccaagaa     1380

ggcgcuguac auaccgcccu agcuggugcc cucgaagcag aaauggacgg agccaaagga     1440

cgccuguucu ccggccaccu caaaugccgc cucaaaaugg acaaacuccg ccucaaagga     1500

guaucauacu cccuaugcac cgcagcuuuc acguucacaa aagugccagc cgaaacacua     1560

cacggaacag ugaccgucga aguccaauac gccggcaccg acggacccug caaaguaccc     1620

gcccagaugg ccguagacau gcagacccuu acccccgucg gaagacuaau caccgcaaac     1680

ccagucauca ccgaaucaac agaaaauagc aaaaugaugc ucgaauuaga cccacccuuc     1740

ggcgacucau acaucgucau uggcguaggc gacaagaaaa uaacccacca cuggcacagg     1800

ucagguucca ccaucggcaa agcauucgaa gccaccguac gaggcgccaa acgcauggca     1860

guccuaggag acaccgcgug ggacuucggc agcgugggag gaguuuucaa cucccucgga     1920

aaaggaaucc accaaaucuu uggcgcggca uuuaaaaguc uauuuggagg aaugagcugg     1980

uucucacaaa uucugauagg cacccuccuc gucuggcuag gucuaaacac caaaaacggc     2040

aguaucucac uaaccugccu cgcacuagga gggguaauga ucuuccucag uaccgcaguc     2100

uccgcagacg uugggugcag cgucgacuuc uccaaa                               2136


<210>  2905
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long CDS opt

<400>  2905
cgaauaauca acgcgaggaa agaacgaaag agacggggcg cggacacauc cauaggcaua       60

guagggcugc ugcuaacaac ggcgauggca gccgaaauaa caagaagggg aagcgccuac      120

uacauguauc ucgaccgauc ggacgccggg aaagcaauca gcuucgcaac cacccucgga      180

guuaacaaau gccacguaca aaucauggac cuaggccaca ugugcgacgc cacaaugagc      240

uacgaaugcc caaugcugga cgaaggcgug gaacccgacg acgucgacug cuggugcaac      300

acgacaagca ccuggguggu cuacggcaca ugccagcaca agaaggggga agcccgacga      360

uccagacgcg cggucacacu gccaagccac uccacccgca aacuacagac aaggagccaa      420

accuggcucg aaagccgaga auacaccaaa caucucauaa aaguagagaa cuggauauuc      480

cgaaacccug gguucgcccu cgucgcggug gcaaucgcau ggcuacuagg auccagcacg      540

agccaaaaag ucauauaccu aguaaugaua cucuuaaucg cgccagcaua cucaauccga      600

ugcauaggcg ucuccaacag agauuucguc gaaggaaugu ccggcggcac cuggguagac      660

gucgugcuag aacauggagg cugcguaaca guaauggcac aagacaaacc aacagucgac      720

aucgagcucg ugacaacaac cguaaguaac auggcggaag ugcgcucgua cuguuacgaa      780

gccagcaucu cugacauggc aagcgauagc agaugcccaa cgcaaggcga agcguaccuc      840

gacaagcaau ccgacacaca guauguaugc aagaggacau ugguagacag agguuggggu      900

aacggaugcg gacuguucgg aaaaggcucg cuaguaaccu gcgccaaauu cacaugcagu      960

aagaaaauga caggcaaaag cauacagccc gagaaccucg aauacaggau aaugcucuca     1020

gugcacggaa gccaacacag cggaaugaua guaaacgaca uuggccacga aacugacgag     1080

aacagagcaa aaguagaagu gacacccaac agcccaaggg cggaggcaac acuaggcgga     1140

uucggcagcc uaggccuaga cugugaaccg cgaacaggcc ucgacuucag cgaccuauac     1200

uacuuaacca ugaacaacaa acacugguug gugcauaaag agugguucca cgacauacca     1260

cuuccauggc acgccggagc agacacaggg accccacacu ggaacaacaa agaagcacug     1320

gucgaguuca aagacgcgca cgccaaacga cagacaguag ucguccuggg aagucaagaa     1380

ggcgcgguac acaccgcacu cgcaggcgcc cuagaagccg aaauggacgg cgccaaagga     1440

aggcuguuca guggccaccu aaaaugcagg cucaaaaugg acaaacuucg acugaaaggg     1500

guaagcuacu cguugugcac cgccguaugc acagccgcca aaguacccgc ggaaacacua     1560

cacggcacgg ugacagugga aguccaguau gccgggaccg acggacccug caagguccca     1620

gcccaaaugg caguagauau gcaaacccuc acgcccguag ggcgacuaau aaccgcaaac     1680

ccaguaauaa cagaaucgac agaaaacagc aaaaugaugc uggaacucga cccgcccuuc     1740

ggagacagcu auauaguaau aggggucgga gacaagaaaa uaacacacca cuggcaccgg     1800

agcggaagca caauugggaa agcauucgaa gcgacagugc ggggagcuaa aaggauggcg     1860

guacuaggag acaccgcaug ggauuucggg agcgucggcg ggguauucaa cagccuaggg     1920

aaaggcauac aucaaauauu uggcgcagcu uucaagagcc uauucggggg caugagcugg     1980

uucucacaaa uacuaauagg aacccuccua gucuggcucg ggcucaacac aaagaaugga     2040

ucaauaagcc uaacaugccu ugcucucgga ggcgugauga uauuccuauc aacagcggug     2100

agcgcggacg ugggaugcuc cguagacuuc agcaaa                               2136


<210>  2906
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long CDS opt

<400>  2906
cgaauaauaa acgcccgcaa ggaaagaaaa cgcaggggcg ccgacgccuc aauaggaauc       60

gucucacucc uacucacaac agugauggcc gcagagauca cgcgcagagg auccgcauac      120

uacauguacc uagacagaag cgacgcagga aaggcaauau ccuucgucac aacccucggc      180

gugaacaaau gccacgucca aauaauggac cuaggccaca ugugcgacgc cacaauguca      240

uacgagugcc caaugcugga cgaaggcguc gaaccggacg acgucgacug cuggugcaac      300

acaacgucca cauggguagu cuacggaacc ugccaccaca agaaaggcga agcacggagg      360

agccggcgcg caguaacauu accaucacac aguacccgaa aacuccaaac cagaucccaa      420

accuggcuag agagccgcga auacaccaaa caccuaauca aaguggaaaa cuggauauuc      480

agaaaucccg gauucgcacu cgucgccgua gccaucgcau ggcuacuggg auccagcaca      540

agccaaaaag uuaucuaccu cguaaugaua cuccugaucg cacccgcaua cucaauaaga      600

ugcauaggcg uaagcaaccg cgacuuugua gaaggaauga gcggagggac gugggucgac      660

gucguacuag agcacggagg augcgucacc gugauggccc aggacaaacc caccgucgac      720

auugaacucg ucacgaccac aguaagcaac auggccgaag uacggucaua cugcuacgaa      780

gccucaauaa gcgacauggc aucagacuca cgcugcccaa cacaagggga agccuaccua      840

gauaagcaau ccgacaccca auacguaugc aaacgcaccc uagucgaccg cggauggggg      900

aacggaugcg gacuauucgg caaaggaucg cuggugaccu gcgccaaauu cacaugcuca      960

aagaaaauga ccgggaaaag cauccaacca gaaaaccucg aauaccguau aaugcuauca     1020

guccacggcu cucaacauuc aggcaugauc gucaacgacg agaaccgcgc aaaaguagag     1080

gugaccccca acuccccaag agcagaggcg acccucggag gauucggcuc acugggccua     1140

gacugcgaac cccgcaccgg cuuagacuuc agcgaccucu acuaccuaac caugaacaac     1200

aaacacuggc uaguacacaa agaaugguuc cacgacauac cacuucccug gcauucaggg     1260

gccgauaccg aaaccccgca cuggaacaau aaagaagccc ucguagaguu caaggacgcg     1320

cacgccaaga gacagacagu cguaguccuu ggaagccagg aaggcgccgu acacacugcc     1380

cucgccggag cauuagaagc cgaaauggac ggcgcgaaag gccgacuauc aucuggccac     1440

cugaaaugcc ggcucaagau ggacaagcug cgucucaagg gagugucaua cucacucugc     1500

acagccgcau ucacauucac caaggucccc gcugaaaccc uccacggaac cgugaccguc     1560

gaaguccaau acgccggacg agacggaccu ugcaagguuc ccgcccagau ggccgucgac     1620

augcaaaccc uaacacccgu cggcagacua aucaccgcca accccgucau cacagaauca     1680

acagaaaacu caaagaugau gcuagaacua gacccaccau ucggggacag cuacauagua     1740

auaggcgucg gagacaagaa aaucacgcac cacuggcacc gcaguggguc aauaauaggc     1800

aaagcguucg aagccacggu ccgcggagcc aaacgcaugg caguacuagg cgacaccgcc     1860

ugggacuucg gaagcgucgg aggcguguuc aacucacucg gcaaaggaau acaucaaaua     1920

uucggcgcug ccuucaaauc ucuauucgga gguauguccu gguucucaca aauacuaauc     1980

ggaacccucc ucgucuggcu cggacuuaac accaaaaacg ggagcaucuc acugaccugc     2040

cucgcccucg gcggcguaau gaucuuccua agcacagcag uauccgcaga cgucggcugc     2100

agcguagacu ucuccaag                                                   2118


<210>  2907
<211>  2118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long CDS opt

<400>  2907
cgcaucauaa acgcccgcaa agaaagaaaa agaagaggag ccgacaccag uaucggaaua       60

guuggacucc uccuaacaac cgccauggcc gccgaaauaa cccgccgcgg cagcgcuuac      120

uacauguacc ucgacaggag cgaugcaggc aaagccaucu cauucgccac aacuuugggc      180

guaaacaaau gccacgugca aaucauggac cucggccaca ugugcgacgc cacuaugucc      240

uacgaaugcc ccaugcucga cgaaggcgua gaaccugacg acguagacug cuggugcaau      300

acaaccagca ccuggguagu cuauggaaca ugccaccaca agaaagguga agcacgccga      360

ucacggagag ccguaacccu acccucucac agcacccgca aacuacaaac ccgcucccaa      420

acauggcuag agucacgcga guacaccaaa cacuuaauca aaguggaaaa cuggaucuuc      480

cguaaccccg gcuucgcccu cgucgcaguc gccauagccu ggcuccucgg cucauccaca      540

ucccaaaaag ucauauaccu aguaaugaua cuccuuauug caccagccua cagcaucaga      600

ugcaucggcg ucucaaaccg agauuucguc gagggcaugu cuggaggaac cugggucgac      660

guaguccucg aacacggagg cugcgucacc guaauggccc aagacaaacc aacaguugac      720

auagagcuag ugaccaccac agugagcaau auggccgaag uucgauccua cugcuacgaa      780

gccagcaucu cagacauggc cagugacucu cgcugcccca cccaaggaga ggccuacuua      840

gacaaacaaa gcgacacaca auacgucugc aagcgcaccc ucgucgaccg cggaugggga      900

aacgguugcg gauuauucgg caaaggcucu cucguuaccu gcgcgaaauu caccuguucc      960

aagaaaauga ccggcaaaag cauccaaccc gaaaaccuag aauacaggau aaugcucagc     1020

guccacggcu cgcaacacuc cggaaugauc gucaacgaug aaaaccgggc caaagucgaa     1080

gucacgccga acucaccaag agcugaagcu acacuaggcg gcuucggaag ccucggccug     1140

gacugcgaac cccgcacagg ccucgacuuc ucagaccucu acuaccucac caugaacaac     1200

aaacacuggc ucguacacaa agaaugguuc cacgacauac cccucccaug gcacgcaggc     1260

gcagacacag gcacacccca cuggaacaac aaagaagcac ucguggaguu caaagacgcc     1320

cacgcaaaac ggcaaacagu cgucguacua ggcagccaag aaggcgcugu ccacaccgca     1380

cuugcaggcg cccucgaagc cgaaauggac ggagcuaaag gcagacucuu cagcggccac     1440

cuaaaaugcc gccucaagau ggacaaacug cgacuuaaag gcgucucgua cucccuaugc     1500

acagcagccu ucaccuucac aaaggucccg gcggaaacac uacacgggac cguaaccguc     1560

gaaguacaau acgcaggaac cgacggcccc ugcaaaguac ccgcacaaau ggcggucgac     1620

augcaaacac ugacccccgu cggacgccug auaaccgcca accccgucau aaccgaguca     1680

acagaaaaca gcaaaaugau gcucgaacua gacccucccu ucggcgauuc uuacaucguc     1740

auaggagucg gcgacaagaa aaucacacac cacuggcaca gauccgguuc aacaaucggc     1800

aaagcauucg aggccaccgu ccgcggcgcc aaacgcaugg cagucuuagg cgacaccgcc     1860

ugggacuucg gcagcguagg cggaguauuc aacagucucg gcaaaggcau ccaccaaaua     1920

uuuggcgccg cguucaaaag ccuguucggg ggcauguccu gguucuccca aauccucaua     1980

ggaacccucc uaguauggcu uggacuaaac accaaaaacg gcuccauauc ccuaaccugc     2040

cucgcucucg gcggggugau gauauuccuc agcacagcag uguccgccga cgucggcugc     2100

uccguugacu ucuccaag                                                   2118


<210>  2908
<211>  2136
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long CDS opt

<400>  2908
cgaaucauaa acgcaagaaa agaaagaaaa aggagaggcg ccgacaccuc aaucgguaua       60

aucggccuac uccucacaac ggccauggcc gcagaaauca caagacgagg cuccgcauac      120

uacauguacc ucgaccguuc agacgcgggc aaagcaauau cauucgccac aacccucgga      180

guaaacaaau gccacgucca aauaauggac cuaggacaca ugugcgacgc cacuaugagc      240

uacgaaugcc caaugcuaga cgaaggaguc gaacccgacg acgucgacug cuggugcaac      300

accaccucga ccuggguagu guacgggacc ugccaccaca aaaaggggga aacccgaaga      360

ucccggagga gugugagccu aagguaccac uacacacgaa aacuccagac cagaagccaa      420

accuggcugg aauccagaga auacaagaaa caccucauaa ugguagaaaa cuggauauuc      480

agaaacccag gauucgcgau agucagcgug gcaaucacau ggcuaauggg aagccucacc      540

ucccaaaaag uaauauacuu agugaugauc guccucauag uaccagccua cagcaucagc      600

ugcaucggag ucagcaaccg cgacuuaguc gaggggaugu cgggcgggac augggucgac      660

guaguacucg aacauggagg cugcguaaca gaaauggcac aagacaaacc caccguagac      720

aucgagcucg uaaccaugac agucucaaac auggccgagg uccggucgua cugcuacgag      780

gcgucccugu cagauauggc aagcgccucc agaugcccua cccaaggcga accaagucuc      840

gacaaacaau cagacaccca aagcguaugc aaaagaacac ucggagacag ggguugggga      900

aauggcugug gaauauucgg aaagggaagc cuaguaacau gcaguaaguu uaccugcugc      960

aagaagaugc caggaaaauc aauacaacca gaaaaccugg aauaccgaau aaugcucccc     1020

guacacgguu cacagcacag cggcaugauc guaaacgaca uuggacacga aacagaugaa     1080

aacagggcca aaguggaagu gacaccgaac agcccacgcg cagaagccac ccucggaggc     1140

uucggcucac ucggacugga cugcgaacca cguacggggc uugacuucag cgaccucuac     1200

uaccugacga ugaacaacaa acacuggcua guacacaaag agugguucca ugacauaccc     1260

cuacccuggc acgccggcgc agauacaggc acgccucacu ggaacaacaa ggaggcacuc     1320

gucgaguuca aagacgccca cgcaaaaagg caaaccguag ucgugcucgg cucccaggaa     1380

ggagcaguac auacagcacu ggccggagcc cuagaagcag aaauggacgg agccaaagga     1440

cgccucuucu caggccaccu gaaaugcagg cucaaaaugg acaaacugag acucaagggc     1500

guaagcuaca gccucugcac agcagccuuc acauucacca aagugcccgc agaaacacuc     1560

cacggaacgg ucaccgucga aguacaaucc gccggaacag acggccccug caaaguaccc     1620

gcacaaaugg cugucgacau gcaaacauua acacccgucg gacgacugau caccgcaaau     1680

ccgguaauca ccgaauccac agaaaauagc aaaaugaugc ucgaacucga cccacccuuc     1740

ggagacagcu acauagucau cggcgucggu gacaaaaaga ucacccacca cuggcacagg     1800

agcggcagca caauaggaaa agccuucgaa gcaaccgugc gaggagcgaa aagaauggca     1860

guguuaggcg acaccgccug ggacuucggc ucaguaggag gaguguucaa cagccuagga     1920

aaaggaauac accaaaucuu cggcgcggca uucaaaaguc uuuucggagg aaugagcugg     1980

uucagccaga uauuaauagg cacccuccuc guauggcuag gucuaaacac caaaaacggc     2040

uccaucucac uaaccugccu cgcgcuaggc ggagucauga ucuuccucag cacagccguc     2100

uccgcagacg uagggugcuc agucgacuuc agcaaa                               2136


<210>  2909
<211>  2130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long CDS opt

<400>  2909
cgcauaauaa acgcccgaaa agaacgcaaa cgaagaggcg ccgacacauc aauaggaaua       60

auaggccucc ucuuaacgac agcgauggca gccgaaauaa ccaggagggg uucagccuau      120

uacauguacc uggaccgcag cgacgcagga aaagccaucu cguucgcaac aaccauggga      180

gucaacaagu gccacguaca aaucauggac cuaggacaca ugugcgacgc cacaaugucc      240

uacgaaugcc cuaugcuaga cgaaggcgua gaacccgacg acgucgacug cuggugcaac      300

accaccagca cauggguggu auacggaacc ugccaccaca agaaaggaga agcgagaccg      360

uccagacgag cgguaacacu gccgagccac uccacccgca aacuucaaac caggucacaa      420

acauggcucg aaagcagaga auauacaaag caccuaauca aagucgaaaa cuggauccuc      480

cgcaacccag gauucacagu ggcagcacua gcaauggccu ggcuacuggg aaacagcacc      540

ucacaaagag ucaucuaccu agucaugauc cuacuaaucg cccccgccua cucgaucagg      600

ugcaucggcg uuagcaaccg ggacuucgua gaaggcauga gcggcggaac augggucgac      660

guagugcucg aacacggcgg gugcgucaca guaauggcac aagacaaacc cacagucgau      720

aucgagcuag ucaccacaac aguaagcaac auggccgaag ucaggucgua cugcuacgaa      780

gcaagcauaa gcgacauggc aagugacagc agaugcccca cccaagggga agccuaccua      840

gacaaacaau ccgacaccca auacgucugc aagcgcaccc uagucgacag agggugggga      900

aacggcugcg gccuauucgg caaaggaucc cuaguaacau gcgcaaaauu uaccugcagc      960

aaaaagauga caggcaaauc aauccaaccc gaaaaccucg aauacagaau caugcucuca     1020

guccacgguu cacaacacuc ggggaugauc gucaacgaua ucggacacga aaccgaugaa     1080

aacagagcaa aggucgaagu cacuccaaac uccccuagag ccgaagcaac ccucggagga     1140

uucggcuccc ucggccuaga cugcgaacca cgcaccgggc ucgacuucag cgaccuauac     1200

uaccuaacaa ugaacaacaa acacuggcug guacacaaag aaugguucca cgacauacca     1260

cuaccuuggc acgcaggggc cgacaccggc acaccccacu ggaacaacaa agaagcgcua     1320

guagaguuca aggacgcaca cgcaaaaaga caaaccgucg uaguacuagg aagccaggaa     1380

ggcgcggucc acaccgcgcu ggccggcgca cuagaagccg aaauggaugg agcaaaaggg     1440

cgccucuucu ccggccaccu caaaugccgg aucaaaaugg acaaguuaag acuaaaaggc     1500

gucucauaca gccuuugcac agcagccuuc acauucucga aagugccggc agagacacug     1560

cacggcaccg uaaccgucga aguacaauau gcaggcagag acggcccaug caagguaccc     1620

gcacaaaugg cagucgacau gcagacacua accccagucg gccgccuaau aacagcaaac     1680

cccaucauca cagaaagcac agaaaacucc cgcaugaugc ucgaacuaga cccacccuuc     1740

ggcgacagcu acauaguaau cggggucggc gacaagaaaa uaacacacca cuggcaccga     1800

agugggagca caaucggaaa agcauucgaa gccacaguca gaggagccaa acggauggca     1860

guccucggcg acaccgcaug ggacuucgga agcgucggag gcgucuucaa cucauugggc     1920

aaaggagucc accaaguuuu uggggccgca uucaaaagcc uguucggagg gaugagcugg     1980

uucucgcaaa uacuaaucgg cacccugcuc gucuggcucg gccucaacac aaagaacgga     2040

ucuaucucac uugcaugccu cgcacucggc ggcgccauga ucuuccuauc cacggcaguc     2100

agcgccgacg uaggcuguag cguagacuuc                                      2130


<210>  2910
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short CDS opt

<400>  2910
acaucaguag gaauaguagg ccugcuacuc accaccgcaa uggccgcgga agucaccaga       60

agagggagcg cauacuacau guaccuagac aggaacgacg cgggagaggc cauaucauuc      120

ccaacuacgc uagguaugaa caaaugcuau auccaaauaa uggaccuagg gcauaugugc      180

gacgccacga ugaguuacga gugccccaug cuagacgaag gggucgaacc ggacgacgua      240

gacugcuggu gcaacacaac cucaaccugg guagucuacg ggaccugcca ccacaagaaa      300

ggcgaagcac gccgaucaag acgagcggua acccucccaa gccacucgac aagaaaacuc      360

caaacccgaa gucaaaccug gcucgaaucu cgcgaauaca ccaaacaccu cauucgagua      420

gaaaacugga ucuuccggaa cccaggcuuc gcguuagccg ccgccgccau agcauggcua      480

cuuggaagcu ccaccuccca aaaagucaua uaccuaguaa ugauccuccu cauagcgcca      540

gccuacucca uuagaugcau aggcguaagc aauagagacu ucgucgaagg caugagcggg      600

ggcacguggg uugacaucgu ccuggagcac ggcggcugcg uaacaguaau ggcccaagac      660

aaaccaaccg uugacaucga gcuagugacg acaaccguau caaacauggc agaaguaagg      720

uccuacugcu acgaagcaag caucuccgac auggcaucag acagcagaug cccgacacaa      780

ggagaagcau accuagacaa gcaaagcgac acccaguacg uaugcaaaag aacgcugguc      840

gaccgcggau ggggaaacgg augcggccua uuuggcaaag gaagccuagu aaccugcgcc      900

aaauucgcau gcuccaaaaa gaugacaggc aaauccaucc aaccagaaaa ccuagaauau      960

cgaaucaugu uaagcguaca cggaagucaa cacucaggca ugaucgucaa cgacacagga     1020

cacgagacag acgaaaacag agcaaaaguc gaaaucacac caaauucacc ccgagcagaa     1080

gccacccuag ggggcuucgg aucgcucggc cuagacugug aaccacgcac cggacuggau     1140

uucagcgacc uauacuaccu aaccaugaau aacaagcacu ggcucguaca caaagaaugg     1200

uuccacgaca uaccacuccc auggcacgcc ggcgccgaca cagggacgcc acauuggaac     1260

aacaaagaag cacucguaga auuuaaagac gcccacgcca aacgacaaac agucgugguc     1320

uugggguccc aagaaggcgc gguccacaca gcacucgccg gcgcccugga agcggaaaug     1380

gacggcgcca agggcagacu gucaucgggc caccuaaaau gccgacuaaa gauggacaaa     1440

cuccgccuca aaggcguauc auacucccuc ugcacggcag cauucacauu caccaaaaua     1500

cccgcggaaa cacuacacgg caccgucaca guagaagucc aauacgccgg aacggacggg     1560

ccguguaagg uccccgccca aauggccguc gauaugcaaa cccugacucc agucggccga     1620

cuaaucacag caaaccccgu cauaacugaa uccacagaga acaguaagau gaugcucgag     1680

cucgacccac ccuucggcga cagcuacaua guaaucggag ucggcgagaa gaaaauaaca     1740

caccacuggc accgcagcgg auccaccaua ggcaaagccu ucgaagccac cguccgaggu     1800

gccaaacgca uggcaguacu gggcgacaca gccugggacu ucggcagcgu cggaggagcc     1860

cuaaacagcc ugggaaaagg cauacaccaa aucuucggag ccgccuucaa aucccuuuuc     1920

ggaggcaugu ccugguucag ccaaauccug aucggcaccc ugcuuaugug gcuaggccua     1980

aacaccaaaa acggauccau cagccuaaug ugccuggccc uaggcggugu gcuaaucuuc     2040

cuaagcacug ccguaucagc u                                               2061


<210>  2911
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short CDS opt

<400>  2911
acuucagucg gcaucguagg ccuacuccuc accaccgcca uggcagccga aguaacccgc       60

cgcggcuccg cauacuacau guaccucgac aggaacgacg cuggcgaagc caucuccuuc      120

cccacaaccc ucggaaugaa uaaaugcuac auccaaauca uggaccucgg gcacacaugc      180

gacgcaacua uguccuauga augccccaug cuagacgaag gcgucgaacc cgaugacguc      240

gauugcuggu gcaacacaac cucaaccugg guagucuacg gaaccugcca ccauaagaaa      300

ggcgaagccc gcagaucccg acgcgccgua acacuaccgu cccacuccac gcgcaaacuc      360

cagacaagga gccagacaug gcuagaaagc cgcgaauaca ccaaacaccu aauaagagua      420

gagaacugga ucuuccgaaa cccaggauuc gcacucgcag cagcagccau cgcauggcua      480

cucggaucca gcaccuccca aaaaguaauu uaccugguca ugauccugcu aauagcgccc      540

gcauacagca ucaggugcau aggggucucc aauagagacu ucguagaagg caugagcggc      600

ggaacguggg uagacgucgu acucgaacac ggcggcugcg ucacuguaau ggcccaagac      660

aaaccuacgg uugacaucga acucguaaca acaaccgucu caaacauggc cgaaguacga      720

uccuacugcu acgaagccuc aauauccgac auggccuccg acagccggug ccccacgcaa      780

ggcgaagcau acuuagacaa acaaagcgac acacaauacg ucugcaaacg aacacugguc      840

gaccgcggcu ggggaaacgg cuguggacua uucggaaaag gcucacucgu aacaugcgca      900

aaauucgccu guagcaagaa aaugacagga aaaagcaucc aacccgaaaa ccucgaauac      960

cgaaucaugc ugucagucca cggaagccaa cauucaggaa ugaucgucaa cgacacagga     1020

cacgaaacug acgaaaacag agccaagguc gagauaacac caaacagccc acgugcagaa     1080

gccacccuag gaggguucgg gucacucggc cuugacugcg aaccgcggac gggacuagac     1140

uucagcgacc uuuacuaccu cacaaugaac aacaaacacu ggcuaguuca uaaagaaugg     1200

uuccacgaua ucccacugcc cuggcacgca ggcgccgaca caggcacacc acacuggaac     1260

aacaaggaag cccucgucga guucaaagac gcccacgcga aaagacaaac cgucgucgua     1320

cuaggcucac aagagggcgc cguccacacc gcccuagccg gcgcccuaga ggcagaaaug     1380

gacggcgcaa agggccgccu cucauccggg caccucaaau gccgccucaa aauggacaaa     1440

cuccgucuua aaggcgucuc guauucccua ugcaccgccg ccuucacguu caccaaaaua     1500

ccggcagaaa cgcuccacgg aaccgucacc guagaaguac aguaugccgg cacggacggc     1560

cccugcaaag ucccagccca aauggccguc gacaugcaga cacucacccc agucggcagg     1620

cuaaucacgg cgaaucccgu caucacagaa uccacagaaa acucaaaaau gaugcuagaa     1680

cuagacccgc cauucggcga caguuacauc gucaucggag ucggcgaaaa gaagaucaca     1740

caucacuggc acagaagcgg cagcacaaua gggaaggccu uugaagcaac cgucagaggu     1800

gccaaaagaa uggcaguccu cggcgacacc gccugggacu ucggaucggu aggcggugcc     1860

cucaacuccc uaggaaaggg cauccaccaa aucuucggug ccgcauucaa aucccuauuc     1920

gggggcaugu caugguucuc acagauacua auaggaaccc uccucaugug gcuaggccua     1980

aacgccaaaa acggcagcau aagccugaug ugccuagccc ucggcggcgu acuaaucuuc     2040

cucagcacag ccgucucagc a                                               2061


<210>  2912
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short CDS opt

<400>  2912
acgagcauag gcauaauagg acucuugcuc acaaccgcaa uggccgccga aaucacgcga       60

cgaggcucag ccuacuacau guaccuagac agaagcgacg caggcaaagc caucucguuc      120

gcaaccacac ucggaguaaa caaaugccac guacaaauaa uggaucucgg acacaugugc      180

gaugcaacaa uguccuacga gugccccaug uuagacgaag gaguagaacc ggacgacguc      240

gacugcuggu gcaauaccac aagcacgugg guaguauaug gcacgugcca ccacaagaaa      300

ggcgaagcac gcagaagccg gcgcgccguc acgcuaccca gccauagcac cagaaagcuc      360

cagacacggu cccaaaccug gcucgagagc cgcgaauaua caaaacaccu gaucaagguc      420

gaaaacugga ucuuccggaa uccuggauuc gcacucgucg ccguugcaau cgcuuggcuc      480

cuaggcucga gcacgagcca aaaaguaauc uaccucguaa ugauacuacu aaucgcgccc      540

gcauauucua uccgaugcau aggcgugagc aaccgugacu ucgucgaagg aaugucgggc      600

gggacauggg ucgauguagu acuagagcac gggggaugcg ucacggucau ggcacaagac      660

aagcccaccg ucgauaucga gcucgucacg accacaguga gcaacauggc ugaagugcgc      720

ucauacugcu acgaagccuc cauaucagac auggcauccg acagcagaug cccuacacaa      780

ggugaagcau accuggacaa acaaucagac acacaauacg ucugcaaaag gacccuaguc      840

gaucgcggau gggggaacgg gugcggacua uucggcaaag gaagccuagu gaccugcgcu      900

aaauuuaccu gcucgaagaa aaugacagga aagagcaucc aacccgaaaa ccucgaauac      960

agaaucaugc ucucagucca cggcucucaa cauagcggga ugauaggcua cgaaacugac     1020

gaggacagag ccaaagucga ggucacgccc aacagcccgc gcgcagaagc uacacuggga     1080

ggauucggca gccuaggacu agacugcgaa ccacgcacag gacucgacuu cagcgaccua     1140

uacuaccuga caaugaacaa caaacacugg cuaguccaca aagaaugguu ccacgauaua     1200

ccgcucccgu ggcacgccgg cgccgacacc gguaccccac acuggaacaa caaagaagca     1260

cucgucgagu ucaaagacgc acacgcaaaa agacaaacgg ucguaguauu aggcucgcaa     1320

gaaggcgcag uccacacagc ccuagcaggc gcgcuagaag cagaaaugga cggcgcgaaa     1380

ggcagacuau ucucaggcca ucucaagugc cgccuaaaaa uggacaaacu ccgccuaaaa     1440

gggguauccu acucacucug cacagcagca uucacauuca ccaaaguccc cgcggaaacc     1500

cuacauggca caguaaccgu agaagugcaa uaugccggaa cggauggccc cugcaagauc     1560

ccgguacaaa uggcgguuga caugcagacu cugaccccag uagggcgccu aaucacagca     1620

aacccuguaa ucacugaauc cacagaaaac ucaaaaauga ugcucgaacu ggacccacca     1680

uucggggaca gcuacauagu aauaggaguc ggcgacaaga aaaucaccca ccacuggcac     1740

agaagcggga guacaaucgg aaaagcguuc gaggccacag ucaggggcgc aaaaagaaug     1800

gcaguacuag gagacaccgc uugggacuuc ggcagcgugg gcgggguauu caacucacuc     1860

ggaaaaggca uccaccaaau cuucggugca gccuucaaaa gccuguucgg aggaaugucc     1920

ugguucuccc aaauucucau cggaacgcuc cuagucuggc ucggacuaaa uaccaaaaac     1980

ggaucuauca gcuuaaccug ccucgcgcua ggaggaguga ugaucuuccu guccacagca     2040

guguccgcc                                                             2049


<210>  2913
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short CDS opt

<400>  2913
accuccguag gcaucguagg ccuacuccug acgaccgcca uggccgccga agucacccgc       60

cgcggcuccg cauacuacau guaccucgac cgaaacgacg ccggagaagc caucagcuuu      120

cccacuaccc ucggcaugaa caaaugcuac auccaaauca uggaccucgg ucacaugugc      180

gacgcuacua ugagcuacga augucccaug cuagacgagg gaguugaacc cgacgacguc      240

gacugcuggu gcaacacaac aucaacaugg gucguauacg gcaccuguca ccacaagaaa      300

ggcgaagcaa gacguucuag acgagccgua acacuaccau cccauucgac ccgcaaauua      360

caaacccgcu cccaaaccug gcuagaauca cgagaauaca ccaaacaccu cauccgcguc      420

gagaacugga ucuucaggaa cccgggauuc gcacuugcug ccgcagcaau agcauggcuc      480

cucggauccu ccaccuccca aaaaguuaua uaccucguca ugauccuccu uaucgccccc      540

gcauacucga uccgcugcau aggugucuca aaccgagacu ucguggaagg cauguccggg      600

ggcacguggg uagacguggu cuuagaacac ggcggcugcg uaaccguaau ggcacaagac      660

aaaccaacag uggacaucga acuggucacc acaacaguau ccaacauggc cgaaguccgc      720

agcuauugcu acgaagcaag cauaucugau auggcguccg acucacgcug ccccacucag      780

ggcgaagccu accuugacaa gcaauccgac acccaauacg ucugcaaacg cacacucguc      840

gaccgaggcu ggggcaacgg augcggacua uucggaaagg gcucccuagu cacaugcgcc      900

aaauucgccu gcuccaagaa aaugaccggc aaaucgauac aacccgaaaa ccucgaauac      960

cguauaaugc uauccgucca cggcucacaa cauucaggga ugaucgucaa cgacacaggc     1020

cacgaaaccg acgaaaaccg agccaagguc gaaauaaccc cgaacagccc cagagccgaa     1080

gccacccuag gaggcuucgg cucccuaggc cuagacugcg aaccccgcac cggccucgac     1140

uucuccgacu uguacuaccu cacgaugaac aacaaacacu ggcuagucca caaagaaugg     1200

uuccacgaca uaccccuccc auggcacgcc ggcgcagaua ccggaacucc ccacuggaac     1260

aacaaagaag cccuagucga guucaaagac gcccacgcca aacgccaaac agucguaguc     1320

cuaggauccc aagaaggcgc cguucacacg gcacucgccg gagcacuaga agccgaaaug     1380

gacggcgcaa aaggccgucu auccucaggc caccuuaaau gccgccugaa gauggauaaa     1440

cuccggcuca aaggagucuc cuacucacug ugcaccgccg cauucacauu caccaaaauc     1500

ccagccgaaa cccuccacgg cacaguaacc guugaagucc aauacgccgg caccgauggc     1560

ccaugcaaag uaccggccca aauggcagua gacaugcaaa cacucacacc cguaggacgc     1620

cucauaacgg ccaacccagu aauaaccgaa uccacagaaa acuccaaaau gaugcuagaa     1680

cuagacccac ccuucgggga cuccuacauc gucaucggcg uaggcgaaaa gaaaaucaca     1740

caucacuggc accgcucagg aucaacaaua ggaaaagccu ucgaagccac aguaagaggu     1800

gcaaaacgca uggcaguacu aggagacaca gcaugggacu uugggucagu cggcggagcc     1860

cugaacucac ucggaaaagg gauccaccaa aucuucggcg ccgcauucaa aucccuauuc     1920

ggaggaauga gcugguucag ccaaauccuc aucgggaccc uacuaaugug gcucggccuc     1980

aacaccaaaa acggcuccau cucccuuaug ugucucgcuc uaggcggcgu acucauauuc     2040

cucagcaccg ccgucucugc a                                               2061


<210>  2914
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short CDS opt

<400>  2914
acgagcaucg gcaucauagg ucuccuccua accacggcca uggccgccga aaucacccgc       60

cgcggcuccg cauacuacau guaccuugac cgcucagaug ccggaaaagc aaucucauuc      120

gcgacaaccc ucggaguaaa caaaugccac guacaaauca uggaccuggg ccauaugugc      180

gacgccacga uguccuacga augccccaug cuagacgaag gggucgaacc cgaugacgua      240

gauugcuggu gcaacacaac aagcaccugg gucgucuacg gcacaugcca ccacaagaaa      300

ggagaagcac gccgaucacg ccgagccguc accuuacccu cucacucaac ccgaaaacua      360

cagacaagau cccagaccug gcuugaaagc cgcgaauaca caaaacaccu caucaaaguc      420

gaaaacugga uauuccggaa ccccggauuc gcccuaguag caguagcaau agcauggcug      480

cucggauccu caacaucaca aaaaguaauc uaccucguca ugauccugcu uaucgcaccc      540

gccuacucaa ucagaugcau aggagucagc aaccgcgacu ucgucgaagg gaugucagga      600

ggcacguggg ucgacguagu ccuagaacac ggcggcugcg ucacaguaau ggcccaagac      660

aaaccuaccg uagacaucga gcucguaacc accacuguau ccaauauggc cgaaguccgu      720

ucauauugcu acgaagccuc aaucuccgac auggcaagcg acucccgaug ccccacccaa      780

ggagaagcgu accucgacaa acaauccgac acccaguacg ucugcaaacg aacuuuggua      840

gaccggggcu ggggcaacgg augcggucuc uucgguaaag gauccuuagu aaccugcgca      900

aaauuuaccu gcagcaagaa aaugacagga aaaucaauac aaccagaaaa ccucgaauac      960

cgcaucaugc ucucagucca cgggucccaa cacuccggaa ugauagucaa ugacacaggc     1020

uacgaaaccg acgaaaaccg cgccaaagua gaagucacac ccaacagccc ccgcgcagag     1080

gccacccucg gcggcuucgg cucccuaggc cuagacugug aaccgcgaac aggccuggac     1140

uucuccgacc ucuacuaccu uacuaugaac aacaaacacu ggcucgucca caaagaaugg     1200

uuccacgaca ucccucuacc auggcacgcc ggagcagaca ccggcacacc ccauuggaac     1260

aacaaagaag cccuuguuga guucaaggac gcccacgcga aacgacaaac cgugguagua     1320

cuugguucac aagaaggcgc aguccacaca gcccuagccg gagcccucga agcagagaug     1380

gacggagcca aaggcaaacu cuucuccggc caccuaaagu gcagacuaaa aauggacaaa     1440

cuaagacuaa aagggguauc auacagccua ugcaccgccg cuuucaccuu cacgaaaguc     1500

ccagccgaaa cccuacacgg caccguaacc gucgaaguac aauacgcagg aaccgacggc     1560

cccugcaaaa uccccgucca gauggccgua gauaugcaaa cacuaacccc cgucggacgc     1620

cuaauaaccg ccaacccagu caucaccgaa agcacugaaa acuccaaaau gaugcuagaa     1680

cucgacccgc cauucggcga cagcuacauc guaaucggag ucggggauaa aaagaucacc     1740

caccacuggc accgcucagg cagcacgaua ggcaaagccu ucgaagccac aguccgcggg     1800

gccaaacgua uggcaguccu aggagacacg gccugggacu ucggcuccgu cggcggaguc     1860

uucaacuccc uaggcaaagg cauccaccaa auauucggcg cggcauucaa aagccuauuc     1920

ggcggcaugu ccugguucuc acaaauccua auaggcaccc uccuaguaug gcuaggacua     1980

aacaccaaaa acggauccau aagccuaacc ugccuagcac uaggcggcgu aaugauuuuc     2040

cuaagcacgg ccgucagcgc a                                               2061


<210>  2915
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short CDS opt

<400>  2915
acgaguaucg guaucauagg ccuacugcug acaaccgcga uggcugcaga gaucacacgu       60

agaggaagcg cauacuacau guaccuggac cgcagcgacg ccggaaaagc aauaucauuc      120

gccacaacgc ucggaguaaa caaaugccac guccagauaa uggaccucgg ucacaugugc      180

gacgcaacca ugucguacga augccccaug cuagacgagg gagucgaacc agacgacguc      240

gauugcuggu gcaacacaac cagcacgugg gucguguacg gaaccugcca cagaaagaaa      300

ggggaagcga gaagaagcag acgagcgguc acacucccua gccacagcac acgcaaacuc      360

caaacccgaa gccaaacgug gcucgaaagc cgagaauaca caaaacaccu caucaaaguc      420

gaaaacugga ucuuccgcaa ccccggguuc gcacucgugg cagucgccau agcauggcuc      480

cugggcagca gcacgagcca aaaaguaauc uaccuaguga ugauacuccu gaucgcccca      540

gccuacucga uacgaugcau aggcgucagc aaccgcgauu ucguggaagg aauguccggc      600

gggacguggg uagacguagu ccuggaacac ggaggcugcg ucaccguaau ggcccaggac      660

aaacccacag ucgauauaga gcuggugacg acaacaguaa gcaacauggc agaaguacgc      720

ucguacugcu augaagcauc gaucucggac auggcaagcg acagccgcug uccaacccaa      780

ggagaggccu accuagacaa acagagcgac acacaguaug ucugcaaaag aacccucgua      840

gacagaggcu ggggaaacgg augcggccuc uucggcaagg gaucgcuagu aacaugcgcc      900

aaauucacau gcuccaagaa aaugacaggc aaaagcaucc agcccgaaaa ccuagaguac      960

cgaauaaugc ugucagucca cggcucacaa cacuccggca ugauaguaaa cgacaccggc     1020

uacgaaacgg augaaaacag agccaaaguc gagguaaccc cgaacucccc acgagcagaa     1080

gcaacucucg gaggguucgg gagccucggg uuagacugcg aaccccgcac cggauuggac     1140

uucagcgacc ucuauuaccu gacaaugaac aacaaacacu ggcucgucca caaagagugg     1200

uuucacgaca ucccccuccc cuggcacgca ggcgccgaca caggaacgcc acacuggaac     1260

aacaaagagg ccuuggucga guucaaagac gcacacgcca aaagacaaac cguggucgua     1320

cucggcagcc aagaaggagc aguacacaca gcgcuagcag gcgcacuaga agccgaaaug     1380

gacggagcaa aagggaaauu guucucuggc caccucaaau gcagacugaa aauggacaaa     1440

cuaagacuga aaggaguguc auacucacug ugcacagcag cauucaccuu cacaaaagua     1500

ccagcggaaa cccuucacgg gacaguaacc guagaagucc aauacgcagg aaccgacggg     1560

ccgugcaaga ucccagugca gauggccguc gacaugcaga cccuaacccc agucgggcga     1620

cucaucaccg ccaacccagu aauaacagaa aguaccgaaa acagcaaaau gaugcuugaa     1680

cuagacccac ccuucggaga cagcuacaua guaauaggcg ugggagauaa gaaaauaacc     1740

caccacuggc acagaagcgg gagcacaaua ggaaaagcau ucgaggccac cgucagggga     1800

gcaaaaagaa uggcaguacu cggagacacc gccugggacu ucgggucugu cggaggagua     1860

uucaacucac uagggaaagg aauccaccaa auauucggcg ccgccuucaa gagcuuauuc     1920

gggggcaugu ccugguucag ccaaauccua auuggaaccc uccuagugug gcuaggccua     1980

aacaccaaaa acggaagcau aagucuaaca ugucuagccc uaggcggcgu gaugauauuc     2040

cuaagcaccg cgguaagcgc c                                               2061


<210>  2916
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short CDS opt

<400>  2916
acaagcaucg gcauaaucgg acuauuacuc acaaccgcaa uggcagccga aauaacccgc       60

cgaggaucug ccuacuacau guaccucgac aggagcgacg ccggaaaagc aauaucauuc      120

gcaacaaccc uagggguaaa caagugccac guacaaauaa uggaccucgg ccacaugugc      180

gacgccacaa ugagcuacga augccccaug cuggacgagg gcguggaacc ggacgacguc      240

gacugcuggu gcaacaccac uuccaccugg gucguauacg gaaccugcca ccacaagaaa      300

ggggaagcac gaagauccag acgagccguc acauuaccaa gccacucaac cagaaaacua      360

cagacaagaa gccaaacaug gcucgaaagc agggaguaca caaaacaccu gauaaaaguc      420

gaaaacugga uauuccgcaa ccccggguuc gcccucgugg ccguagcaau agccuggcua      480

cuaggcucca gcacaagcca aaaagucauc uaccuaguca ugauccuccu aaucgcgcca      540

gcauacucca uccgguguau aggcgucuca aacagagacu ucgucgaagg gaugucaggc      600

ggaaccuggg uagacguggu acucgagcac ggaggcugcg uaacagucau ggcccaagac      660

aaacccaccg ucgacaucga acugguaaca accaccguaa gcaacauggc ugaaguccga      720

uccuacugcu augaagccag caucucagac auggcaagug acucacgaug cccaacccaa      780

ggcgaagccu accuggacaa gcaaagcgac acccaguacg ucugcaaacg cacccuaguc      840

gaccgagggu ggggaaacgg cugcggccuc uucggaaaag gaucgcuagu cacgugugca      900

aaguuuaccu gcuccaagaa aaugacaggc aaaaguauac agccugaaaa ccucgaguau      960

cgaaucaugc uaucugugca cggcucacaa cacucaggaa ugacggucaa cgacauagga     1020

uacgagaccg acgaaaaccg cgcaaaaguc gaggucaccc caaacagccc acgggcagaa     1080

gccacccucg gaggguucgg cagccuaggc cucgauugcg aaccaagaac aggccuugac     1140

uucuccgacc uauacuaucu caccaugaac aacaaacacu ggcuggucca caaagaaugg     1200

uuccacgaca uaccacuccc cuggcacgca ggcgcggaca cagggacgcc acacuggaac     1260

aacaaagaag cccuaguaga guucaaagac gcacacgcca aacgacaaac cgucgucguu     1320

cuaggcuccc aagaaggcgc aguccacacg gcccuagcag gagcgcuaga agccgagaug     1380

gacggcgcca agggcaaacu cuucagcgga caccuaaaau gcaggcuaaa aauggacaaa     1440

cuacggcuca agggaguauc auacucgcug ugcaccgcgg cguucaccuu caccaaagua     1500

cccgccgaga cccuccacgg aaccguaacc gucgaagucc aauacgcagg gacugacggc     1560

ccgugcaaaa ucccagucca aauggcaguc gacaugcaaa cacuaacacc agucgggcga     1620

cucaucacag caaaccccgu aaucacagaa uccaccgaaa acuccaagau gaugcucgag     1680

cuagacccgc cauucggaga cagcuacauc guaauaggag ucggagacaa aaagaucacc     1740

caccacuggc accgcucggg aagcacaauc ggcaaggccu ucgaagcaac uguaagaggc     1800

gcaaaacgca uggccguacu aggagacacc gcuugggacu ucggcagcgu cggcggggug     1860

uucaacuccc uaggaaaagg cauacaccaa auauucggag cagcuuuuaa aucccuguuc     1920

ggcggcauga gcugguucag ccaaauacua auaggcaccc ucuuagucug gcuaggacuc     1980

aacacaaaga acggcucaau cucacuaacc ugccucgcac uaggaggagu caugauauuc     2040

cugagcacag ccgucagcgc c                                               2061


<210>  2917
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short CDS opt

<400>  2917
acaagcauag gcauagucgg ccugcuacuc accacugcca uggccgccga aauaacacgc       60

agaggcuccg ccuacuacau guaccucgac cgcuccgacg caggaaaagc caucuccuuc      120

gcaaccacgc ucggcgucaa caaaugucac guacaaauaa uggaccuagg ccacaugugc      180

gacgccacca ugagcuacga augccccaug cucgacgagg gcgucgagcc ggacgacgua      240

gacugcuggu gcaacacaac cucuacaugg guagucuaug gaaccugcca ucacaagaaa      300

ggcgaggccc gacgaagccg acgagcaguu accuuaccca gccacagcac acgcaaacuc      360

caaacaagaa gccaaacaug gcuagagagc cgagaauaca caaaacaccu caucaaaguc      420

gaaaacugga uauuccggaa uccaggcuuc acacucgucg caguagcaau agccuggcuc      480

cucggcagca gcaccagcca aaaaguaauc uaccuaguca ugauccuccu aauagcgcca      540

gccuacucca uaaggugcau aggaguaagc aaucgcgacu ucguagaagg aaugagcgga      600

ggcaccuggg ucgacguagu ccucgaacac gggggaugcg uaacaguaau ggcccaagac      660

aagccaacag uagacauaga gcucgucaca acgaccguca guaacauggc agaagucagg      720

ucuuacugcu acgaagcaag caucagcgac auggcaucag acagccgaug ccccacccaa      780

ggagaagcgu accuagacaa gcaaucagac acccaauacg ucugcaaaag gacccuugua      840

gauagaggau ggggaaacgg augcggccuc uucggcaagg gcagccuagu aaccugcgca      900

aaauucacau gcucaaagaa aaugaccggc aaaucaaucc aaccagaaaa ccuugaauac      960

cgaaucaugc ucuccgucca cggaagccaa cacagcggaa ugauagucaa cgacgaaaac     1020

agagcaaaag ucgaagucac ucccaacuca ccccgagccg aagccacccu gggcggcuuc     1080

ggaucccugg gacuugacug cgaaccucgc accggccuag acuucagcga ccuauacuac     1140

cucacaauga acaacaaaca cuggcuaguc cacaaagaau gguuucacga cauaccccuc     1200

ccauggcacg ccggcgcaga caccggaaca ccccauugga acaacaaaga agcccuaguc     1260

gaguucaaag acgcacacgc aaaacggcag acaguagucg uacuaggcuc ccaggagggc     1320

gcaguacaca ccgcacuggc cggcgcccug gaagccgaaa uggauggcgc aaaaggaagg     1380

cuguucucgg gacaccucaa augcaggcug aaaauggaca aacuaagacu caaaggggua     1440

ucauacuccu uaugcacagc cgccuucacc uucaccaaag uaccagcaga gacgcuccac     1500

ggcaccguga ccgucgaggu acaauacgcc ggcaccgacg gaccaugcaa aguccccgcc     1560

caaauggcag uagauaugca gacacugacc ccagucggca gacuaauaac cgccaacccg     1620

guuauaaccg aaagcaccga aaacucaaaa augauguugg aacuggaccc acccuucggc     1680

gauagcuaca uagucauagg uguaggagac aagaaaauca cccaccacug gcacagauca     1740

gguagcacaa ucggcaaagc guucgaagcc accguccgcg gcgcaaaaag gauggccgua     1800

cucggcgaca ccgccuggga cuucggcagc gugggaggag ucuucaacag ccuaggaaaa     1860

ggcauccacc aaaucuucgg agccgccuuc aaaagccucu ucggaggaau gagcugguuc     1920

ucccaaaucc ucaucggaac ccuacugguc uggcucggcc ucaacaccaa aaacggcagc     1980

aucucucuaa ccugccucgc acuaggcggc gucaugauau uccucagcac cgcggucuca     2040

gca                                                                   2043


<210>  2918
<211>  2049
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short CDS opt

<400>  2918
accucaaucg gaaucaucgg acuauugcuc accaccgcga uggcagcaga aaucacacgc       60

agaggauccg cauauuacau guaccuugac cgcagugacg ccggcaaagc aaucucuuuc      120

gccacaacac ucggagucaa caaaugccac guccaaauca uggaccucgg ccacaugugc      180

gacgccacaa uguccuacga augccccaug cucgacgaag gcgucgaacc cgacgacguc      240

gacugcuggu gcaacaccac auccacuugg gucgucuacg gaaccugcca ccacaagaaa      300

ggcgaggcga gacgcuccag acgcgccguc acacuacccu cgcacuccac aaggaaacuc      360

cagacccgaa gccagaccug gcuagagucc cgggaauaca ccaaacacuu aaucaaagua      420

gagaacugga uauuuagaaa ccccggauuc gcucucgucg ccgucgcaau ugcuuggcuc      480

cuaggcucaa gcaccaguca aaaaguuauu uaccuaguca ugauacuacu aaucgcaccc      540

gccuacucca uccgcugcau cggagucagu aaccgcgacu ucguggaagg cauguccgga      600

ggaacguggg ucgacguagu ccucgagcac ggaggaugcg ucacagucau ggcacaggac      660

aaaccaaccg ucgacauaga gcuagucacc acgacagucu caaacauggc cgaaguaagg      720

ucuuacugcu acgaagccag caucuccgau auggccucag acucccgaug ccccacccaa      780

ggagaggccu accucgacaa acaaucagac acccaauacg uaugcaaaag aacccuaguu      840

gaccgcggcu ggggaaacgg cugcggccuc uucggcaaag ggagccuagu aaccugcgca      900

aaauucacau gcuccaagaa aaugaccggc aaauccaucc aaccagaaaa ccucgaguac      960

cgcauaaugc ucucaguaca cggcucacaa cacagcggaa ugauaggcua cgaaaccgac     1020

gaaaaccgcg ccaaagucga agucaccccc aacagcccac gcgccgaagc cacacucggc     1080

ggauucggcu cccuaggccu agacugcgaa ccccgcaccg gucuugauuu uucagaccuu     1140

uauuaccuca caaugaacaa caaacacugg cucgugcaua aagaaugguu ucacgauaua     1200

ccacuaccgu ggcaugcggg agcagacacc ggcacgcccc acuggaacaa caaagaagca     1260

cucgucgagu ucaaagacgc ccaugccaaa cgccaaaccg uaguaguucu aggaucacaa     1320

gaaggagcag uccacaccgc ccucgccgga gccuuagaag cagaaaugga cggagcgaaa     1380

ggcaaacugu ucagcggcca ccuaaagugc agacucaaaa uggacaagcu ccgccucaaa     1440

ggcgucucau acucacuaug caccgcagcc uucaccuuca ccaagguccc agcagaaacc     1500

cuccacggga ccgucacugu cgaaguccag uaugcaggca cggacggacc augcaagauc     1560

ccgguccaga uggcaguaga caugcaaacc cucacacccg ucggccgacu caucacagca     1620

aaccccguga ucacagaauc cacugaaaac ucaaaaauga ugcucgaacu cgacccgccc     1680

uucggcgaca gcuacaucgu caucggcguc ggcgacaaaa agauaaccca ccacuggcac     1740

cgcagcgggu caacuaucgg caaagcauuc gaagccaccg uccgcggcgc caaacgaaug     1800

gccguacucg gagacacggc cugggauuuc ggcuccguag gcggaguauu caacagccua     1860

ggaaagggca uccaccaaau cuucggagca gcauucaaaa gccuauucgg cggcauguca     1920

ugguuuuccc aaauacugau cggcacacuc uuagucuggc ucggacucaa caccaagaac     1980

ggcuccauca gccuaaccug ccucgcacua ggcggaguca ugaucuuccu aagcaccgcc     2040

guaagcgca                                                             2049


<210>  2919
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short CDS opt

<400>  2919
accuccgucg gaauagucgg ccucuuacua accacugcca uggccgcaga aguaacccgc       60

cgagggagcg cauacuacau guaccucgac cgcaacgacg caggagaagc caucagcuuc      120

cccacgacgc uaggcaugaa caagugcuac auacagauca uggaccuagg ccauaugugc      180

gacgccacaa ugucauacga augcccaaug cuagacgaag gcgucgaacc cgacgacguc      240

gacugcuggu gcaauacaac aagcacuugg guagucuacg gaaccugcca ccacaagaaa      300

ggggaagcac gacgaagccg ccgagcaguc acucuccccu cacacagcac ccggaaacuc      360

caaacacgca gccaaaccug gcuagaaagc cgagaauaua caaaacaccu cauacgagua      420

gaaaacugga ucuuccgaaa cccaggcuuc gcauuagccg ccgcggccau cgccuggcua      480

cugggcucgu caacaucaca gaaaguuauc uaccuaguaa ugauacuucu aaucgccccc      540

gccuacagca uaagaugcau cggcguaagc aacagagacu ucguagaggg gaugagcggc      600

gggaccuggg uagacgucgu ccuggagcac ggcgguugcg ucacgguaau ggcccaagac      660

aaaccaaccg uugacaucga acucguaacg acgaccgugu ccaacauggc agaaguccga      720

ucauacugcu acgaagccag caucagcgac auggcauccg acucuagaug cccgacccag      780

ggagaagccu accuugacaa acaaagugac acacaauacg ucugcaaacg aacccucguc      840

gaccgcggcu ggggcaacgg cugcggccuc uucgggaaag gcagccuagu aaccugugcg      900

aaauucgcau gcagcaagaa aaugacaggg aaauccauac agccagaaaa ccuagaauac      960

cgcauaaugc ugagugucca cggaucucaa cacagcggca ugaucguaaa cgacacagga     1020

cacgaaacag acgaaaaccg agcaaaagua gagaucaccc cgaacagccc ccgagcggaa     1080

gcaacacuag gcggauucgg cagccugggc cucgacugug aacccagaac aggcuuagac     1140

uucagcgacc ucuacuaccu caccaugaac aacaaacacu ggcucgucca uaaagaaugg     1200

uuccacgaca ucccgcuccc auggcacgcc ggcgcagaca caggaacgcc acacuggaac     1260

aacaaagagg cgcuagugga guucaaagac gcacacgcca aacgccagac cguagucgua     1320

cuaggaucac aagaaggcgc cguucacaca gcccuagccg gagcacucga agccgaaaug     1380

gacggcgcaa agggcagacu aucaucaggc caccuaaaau gccgccuaaa gauggacaaa     1440

cugcgccuaa aaggcguguc auacucacua ugcacagcag cauucacguu uaccaagaua     1500

ccagcagaaa cacuacacgg uacagucacc gucgaagucc aauacgcagg gaccgacgga     1560

ccaugcaaag uacccgccca aauggcagug gacaugcaaa cacucacacc gguaggacga     1620

cuuaucaccg ccaacccggu aauaacagaa uccaccgaaa acagcaaaau gaugcucgaa     1680

cucgacccac ccuucggaga cuccuacaua guaauaggcg ucggagaaaa gaaaaucaca     1740

caccacuggc accgcuccgg cuccaccauc gggaaagcau ucgaagccac agucagagga     1800

gccaaacgaa uggcgguccu gggcgacacg gcaugggacu ucggaagcgu cgggggcgca     1860

cucaacucac ucggccgugg aauacaccaa aucuucgggg ccgcguucaa aagccucuuc     1920

ggcggcauga gcugguucuc gcaaauccuc auaggcaccc uacucaugug gcucggacua     1980

aacaccaaaa acggaucaau cagccucaug ugcuuagccc uaggcggcgu ccucauuuuu     2040

cucagcaccg cgguaagcgc a                                               2061


<210>  2920
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short CDS opt

<400>  2920
accuccguag gaaucguugg cuuacuccuc accaccgcaa uggcagcaga aguaacucgg       60

cgcggcucag ccuacuacau guaccucgac cgcaacgacg caggagaagc cauaagcuuc      120

cccaccaccc uuggcaugaa caaaugcuac auccaaauaa uggaccuugg ucacaugugc      180

gacgcgacca ugagcuacga augccccaug cuagacgaag gagucgaacc agacgacgua      240

gacugcuggu gcaacaccac aagcaccugg gucguauacg gcaccugcca ccacaaaaaa      300

ggcgaagcca gacgcucacg acgagccguc acucuucccu cccacuccac ccgcaaacua      360

caaacgcgau cccaaaccug guuagaaagc cgcgaauaca ccaaacaccu uauaaggguc      420

gagaacugga uauuccgcaa ucccggacua gcucuugcag ccgcagcaau agccuggcua      480

cuaggaucgu ccaccucaca aaaagucaua uaccuaguca ugauccuccu aauagcucca      540

gccuacagca uacguugcau cggcguuucc aaccgggacu ucguagaagg caugagcggc      600

ggcaccuggg ucgacguugu acuggaacac ggaggaugcg ucaccgucau ggcccaagac      660

aaacccacug ucgacaucga acuaguaacc acaaccguca gcaacauggc cgagguacgc      720

agcuacugcu acgaggcguc caucagcgac auggcguccg acucacggug ccccacccaa      780

ggcgaagcau accuagacaa acaaucugac acccaauacg uaugcaaacg aacccucgug      840

gaccgaggau ggggcaacgg cugcggccug uucggcaaag gcucacuagu caccugugca      900

aaauucgccu gcuccaaaaa aaugacaggg aaauccauac aacccgaaaa ccuagaauau      960

agaaucaugc uaagcgugca cgguucccag cauuccggca ugauaguaaa cgacaccggc     1020

cacgaaaccg acgaaaaccg ugcaaaaguc gaaauaacuc caaacucacc ccgcgccgaa     1080

gcaaccuuag gcggcuucgg cucacucggc cuagacugcg agccccgcac cggguuagac     1140

uucuccgacc uguacuaccu uaccaugaac aacaaacacu ggcuaguaca caaagaaugg     1200

uuccacgaca uaccccuacc auggcacgcg ggggccgaca caggaacccc ccacuggaac     1260

aauaaagaag cccucgucga auucaaagac gcccacgcua agcgccaaac cguagucguc     1320

cuagggucac aggagggagc cguccacacu gcccuagccg gcgcucucga agcagaaaug     1380

gauggcgcaa aagggcgacu uagcuccgga caccuaaaau gucgccucaa aauggacaaa     1440

uugcgacuua aaggagucuc uuacucucuc ugcaccgccg ccuucacauu cacaaaaauc     1500

ccagccgaaa cccuccacgg caccgucacc guagaagucc aauaugcagg caccgacgga     1560

cccugcaaag ucccugcaca aauggccguc gacaugcaaa cacucacacc aguaggccga     1620

cucauaacug ccaacccggu aaucaccgaa uccacugaaa acucgaaaau gaugcucgaa     1680

cucgacccac cauucggaga cucauacaua gucauaggug ucggagaaaa aaaaauaacc     1740

caucacuggc acagaucagg cucaaccauc ggcaaggcau ucgaagccac cguaaggggc     1800

gcaaaacgca uggccguccu aggagacacc gcaugggacu ucggaucggu aggcggcgca     1860

cucaacagcc uaggcaaagg cauacaccag auauucggcg cagccuuuaa aagccucuuc     1920

ggcggaaugu caugguucag ccagauccuc auaggcacac uccuuaugug gcuaggccua     1980

aacacaaaaa acggauccau cucccucaug ugccucgccc ucggaggagu acugaucuuc     2040

cuaucgaccg cagucagcgc c                                               2061


<210>  2921
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short CDS opt

<400>  2921
acaagcguag guauagucgg ccuccuccuc accaccgcaa uggcagccga ggucacacgc       60

agaggcagcg cauacuacau guaccuagac cggaacgacg caggagaagc aaucucguuc      120

ccaaccaccc uugggaugaa caaaugcuac auacagauaa uggaccuggg acauaugugc      180

gacgccacaa uguccuacga augcccaaug cucgacgagg ggguagaacc cgaugacgua      240

gacugcuggu gcaacaccac aagcacaugg gucgucuacg gcacaugcca ccacaagaaa      300

ggggaagccc ggaggagcag acgagcaguc acacucccca gccacagcac aagaaaacua      360

cagacccgcu cacaaaccug gcuagaaagc cgagaguaca caaaacaccu cauacgaguu      420

gaaaacugga ucuuccgcaa ccccggauuc gcgcuagccg cggcagcaau agcauggcuc      480

cugggaucua gcaccaguca aaaaguuauc uaccuuguca ugauacuccu aauagccccc      540

gcauacagca uacgaugcau aggcguaagc aaccgcgacu ucgucgaagg gaugucagga      600

gggaccuggg ucgacguagu acuagaacac ggcggaugcg ugacagucau ggcccaagac      660

aaacccacag uagacauaga gcucgucaca accacaguau ccaacauggc cgaaguccgc      720

ucguacugcu acgaagcauc aaucagcgac auggccucag acucccgcug ccccacgcag      780

ggagaagcau accucgacaa acaaucagac acacaguacg ucugcaaaag gacccuagua      840

gaccgaggau gggggaacgg gugcggacuc uuuggaaagg gaucgcucgu aacaugcgca      900

aaauucgccu gcagcaaaaa gaugacaggc aaaucaauac agccggaaaa ccucgaauac      960

cggaucaugc ucuccguaca cggcucacaa cacucgggca ugauagucaa cgacacaggc     1020

cacgaaacag acgaaaacag ggccaaaguc gaaaucaccc cgaacucacc gcgcgcagag     1080

gcuacccuag gaggguucgg aucccucggc cuagacugcg aaccgcgaac aggacuggac     1140

uucagugacc ucuacuaccu aaccaugaac aacaaacacu ggcuagucca caaagaaugg     1200

uuccacgaca uaccccuccc cuggcacgca ggagccgaua ccggcacgcc gcacuggaac     1260

aacaaagagg cccuagucga guucaaagac gcacacgcaa aaaggcaaac ggucgucgua     1320

cucggcucgc aagaaggagc aguacauaca gcacuagcag gcgcacucga ggccgaaaug     1380

gacggagcca aagggcgccu uuccagcggc caccucaaau gccggcuuaa aauggacaaa     1440

cuuaggcuaa aaggggucuc cuacagccuc ugcaccgccg ccuucacauu cacuaaaauc     1500

ccagcagaga cccuacacgg aacagugacc guagaagugc aauacgccgg gacagacgga     1560

cccugcaaag uacccgcaca aauggccguu gacaugcaga cacuaacccc cgucggacga     1620

cuaaucaccg ccaauccggu gauaaccgaa ucgacugaaa acagcaaaau gaugcucgaa     1680

cucgacccac ccuucggcga cagcuacauc guaauaggag ucggcgaaaa gaaaauaacc     1740

caccacuggu accgcuccgg aagcaccauc ggcaaagcgu ucgaagccac ggucagaggu     1800

gcaaaacgca uggccguccu aggcgauacg gccugggacu ucggaagcgu ugggggagca     1860

cuaaacucac uaggcaaagg gauacaccaa auauucggug ccgcauucaa aucccuguuc     1920

ggaggaaugu caugguucuc acaaauacua auaggcacac uacuaaugug gcugggccuc     1980

aacaccaaga acggaagcau uucccuaaug ugccucgccc ucggcggcgu acuaaucuuc     2040

cuauccacag caguaagcgc a                                               2061


<210>  2922
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short CDS opt

<400>  2922
acgucuguag gcauaguugg ccuacuccuc acaacagcca uggcagccga aguaacccgc       60

cgcggcucag ccuauuacau guaccuggac cgaaacgacg cuggagaagc caucucauuc      120

cccaccaccc ucggaaugaa caaaugcuac auacaaauca uggaccucgg acacaugugc      180

gacgcgacca ugaguuacga augcccaaug uuggacgaag gagucgagcc cgacgacguu      240

gacugcuggu guaacaccac caguacuugg gucgucuacg gaaccugcca ccacaagaaa      300

ggggaagccc gacgaucccg ccgggcggua acccuaccuu cccacagcac ccgcaaacuc      360

caaacacgau cacaaaccug gcucgaaagc agagaauaca cgaaacaucu cauacgagua      420

gagaacugga uauucagaaa uccgggauuc gcacuagccg ccgccgccau cgccuggcuc      480

cugggaucau caaccagcca aaaaguaaua uaccucguga ugauccuccu aauagccccc      540

gcguacucaa uacgcugcau aggcgucagc aaccgggacu ucguagaagg cauguccggg      600

ggcaccuggg ucgacguagu acucgagcac ggcggcugcg ugaccguaau ggcccaagac      660

aaacccaccg uagacaucga acugguuaca accacagugu caaacauggc agaagucaga      720

agcuacugcu acgaagccuc gauaagcgac auggccuccg acagccgaug ccccacccaa      780

ggagaagccu accuagacaa acaaucagac acacaauacg ucugcaaacg aacccuagua      840

gaccgcggcu ggggcaacgg augcggccuu uucggaaaag gcucacuugu aaccugcgcc      900

aaauucgcau gcagcaagaa aaugaccgga aaaucaaucc aaccagaaaa ccuagaguau      960

cgcaucaugc ucuccguaca cggaucacaa cacucaggca ugauagucaa ugauacgggc     1020

cacgaaaccg acgaaaauag agcaaaaguc gagaucaccc ccaacagccc ccgcgccgaa     1080

gcaacacucg gaggauucgg gagccucgga cucgacugcg aaccccgaac aggccuagac     1140

uucucagacc uauacuaccu caccaugaac aacaaacacu ggcucguaca caaagaaugg     1200

uuucacgaca uaccacuacc cuggcacgcu ggggccgaca ccgggacucc ccacuggaau     1260

aacaaagaag cccuaguaga guucaaagac gcccacgcca aacgccaaac ggucguagua     1320

cucggcagcc aagaaggggc cguccacacc gcgcucgccg gagcccucga agccgaaaug     1380

gacggagcaa agggacggcu auccucaggg caccucaaau gcagacuaaa gauggacaaa     1440

cuccgucuga agggcguuuc cuauagccuc ugcaccgcag cauucaccuu cacaaagauc     1500

ccggccgaaa cacuccacgg gaccgucacc guagaagugc aauacgccgg cacagauggc     1560

cccugcaaag uccccgccca gauggccguc gacacccaaa cgcugacacc cgucggccgc     1620

cuaaucaccg cgaaucccgu cauaacugaa uccaccgaaa acucgaaaau gauguuagaa     1680

cucgaucccc cauucgggga cucauacauc guaauaggcg ucggagaaaa gaaaauaacc     1740

caccacuggc accgaucagg cagcaccaua ggcaaagccu ucgaagccac uguaaggggc     1800

gcaaaacgca uggcaguacu aggagacacc gcaugggacu uuggcucagu cggcggagcc     1860

cucaacuccc uaggcaaagg aauacaccag auuuucggag cagcuuucaa aagccucuuc     1920

ggcggcauga gcugguucuc ccagauucua auaggcaccc ugcucaugug gcucggacug     1980

aacaccaaaa acggauccau cucccucaug ugccuagcac ugggcggcgu ccuaauauuc     2040

cugucuaccg ccguauccgc c                                               2061


<210>  2923
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short CDS opt

<400>  2923
acauccgugg gcauagucgg ucuccuacuu accacagcaa uggcagccga aguaacacgc       60

cgaggcucag cauacuacau guaccucgac cggaacgacg caggcgaagc aauauccuuc      120

ccaaccacac ucggcaugaa caaaugcuac auccaaauca uggaccucgg ccacaugugc      180

gacgccacaa uguccuacga augccccaug cucgacgaag ggguggaacc agacgacguc      240

gacuguuggu gcaacaccac cuccaccugg gucgucuacg ggaccugcca ccacaagaaa      300

ggcgaagccc gccgaagcag acgcgcaguc acacucccuu cccacucaac ccgcaaacug      360

caaacccgcu cacaaaccug gcucgaaagc cgcgaauaca ccaaacaccu uauaagagua      420

gaaaacugga ucuuccgaaa cccaggcuuc gcacucgccg cagccgccau agccuggcua      480

cuugggagcu ccacguccca aaaaguaaua uaccucguaa ugauucuccu aauagcgcca      540

gccuacucca uccggugcau aggagucuca aacagagacu ucgucgaagg uaugucggga      600

ggaaccuggg ucgacguagu acuagaacac gggggcugcg ucacugucau ggcacaagac      660

aaaccgaccg ucgacauaga acucguaaca accaccgugu cuaacauggc cgaaauccga      720

agcuacugcu acgaagcaag cauaagcgac auggccuccg acucgcgaug cccaacccaa      780

ggagaagccu accuugacaa acaaagugau acacaauacg ucugcaagag aacauuagua      840

gaccgaggau gggggaacgg augcggccua uucggcaaag gcucacucgu cacgugcgcc      900

aaguucgcuu gcuccaagaa aaugaccggc aaaagcauac aaccugaaaa ccucgaauac      960

agaauaaugc uauccgucca cggcucccaa cacagcggaa ugaucgucaa cgacacagga     1020

cacgaaacag acgaaaaccg cgccaaagua gaaaucaccc ccaacucacc ccgagcagaa     1080

gccacucucg gcggcuucgg cucccuagga cuagacugcg aaccccgaac cggccuggac     1140

uuuuccgacc ucuacuaccu gaccaugaau aacaaacacu ggcuaguaca caaagaaugg     1200

uuccacgaca uacccuuacc cuggcacgcc ggcgccgaca ccggcacacc ccacuggaac     1260

aacaaagaag cccuggucga guucaaggac gcacacgcaa aacgucaaac cguaguaguc     1320

cucggcuccc aagaaggcgc cguccacacc gcccuagcag gggcacuaga agcagaaaug     1380

gacggggcca aaggaagacu auccagcggc caccucaaau gccgacucaa aauggacaaa     1440

cucagacuca aaggaguauc auacagccua uguaccgcag cguucaccuu caccaaaauc     1500

ccagccgaaa cacuacacgg gacaguaacc guagaaguac aauacgccgg aacugacgga     1560

cccugcaaag uacccgcaca aauggcugug gacaugcaaa ccuuaacccc agugggcaga     1620

cugaucacag ccaaccccgu aauaacggaa ucgacggaaa acuccaaaau gaugcucgaa     1680

cuagacccuc ccuucggcga cucauacauc gucaucggcg ucggcgagaa aaagaucacc     1740

caccauuggc accgaagcgg cagcacgaua ggcaaagcau ucgaagcaac aguaagaggc     1800

gccaaacgaa uggccguccu aggagacacc gcaugggacu ucggcagcgu aggaggcgca     1860

cuaaacucac ucggaaaagg aauccaccaa aucuucggag cagccuucaa aucccuauuc     1920

gggggcauga guugguucuc ccaaauacuc aucggcaccc uacuuaugug gcucggccuu     1980

aacaccaaga acggaagcau aagccugaug ugccucgccc ugggcggcgu ccucaucuuc     2040

cuuucuaccg caguaucagc c                                               2061


<210>  2924
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short CDS opt

<400>  2924
accagcgugg gaaucgucgg acuacuccua acaaccgcga uggcugccga aguaacccga       60

cgcgggagcg ccuacuacau guacuuagac agaaacgacg caggggaagc aaucagcuuc      120

ccuacaacgc ucggaaugaa caaaugcuac auacaaauaa uggaccucgg acacaugugc      180

gacgcaacaa ugaguuacga augcccaaug cuagacgaag gcguagaacc agacgacgua      240

gacugcuggu gcaacaccac gagcacgugg guagucuacg guacguguca ccacaagaaa      300

ggcgaagcac gacgcuccag gagagccgua acgcucccga gccacucgac acgaaaacuc      360

caaacacgca gccaaaccug gcuagaauca cgagaauaca cgaagcaccu aauccgagua      420

gaaaacugga uauuccgaaa ccccggauuc gcgcucgccg ccgcggccau cgcauggcuc      480

cuugggagca gcacaagcca aaaagugaua uaccuaguaa ugauccuacu caucgcccca      540

gcauacagua uacgaugcau cggagugagc aaccgcgacu ucguggaagg aauguccggg      600

ggcaccuggg ucgacgucgu ccuggaacac ggcggaugcg ucacggucau ggcacaagac      660

aaaccaacag uggacauaga acucgucacc accacaguca gcaacauggc agaaguccgc      720

agcuacugcu acgaagccag caucucagac auggccuccg acagcagaug ccccacccaa      780

ggcgaagcgu accuagacaa acaguccgac acgcaauacg ucugcaaacg gacacucgua      840

gacagaggau ggggaaacgg cugcggccug uucggcaaag gcucccuagu caccugugca      900

aaguucgccu gcagcaagaa aaugacaggc aaaucaaucc agccggaaaa ccucgaauac      960

agaauaaugc ucagcgucca cggcucccaa cauagcggca ugauaguaaa cgacacgggc     1020

cacgaaacag acgaaaaccg agccaaaguc gaaauaacac ccaacagccc ccgcgcggaa     1080

gccacacucg gaggcuucgg gucgcuagga cucgacugug aaccaagaac aggccuggac     1140

uucagcgacc ucuacuaccu caccaugaac aacaaacacu ggcuugugca uaaagaaugg     1200

uuccacgaca uaccacuccc guggcaugcc ggagcagaca ccggaacacc acacuggaac     1260

aacaaagaag cccuagugga guucaaagac gcacacgcaa aacgacaaac aguagucgua     1320

cuaggauccc aagaaggcgc uguacacacc gcacuagccg gcgcacuaga agccgaaaug     1380

gacggagcaa aaggacgccu auccucaggc caccucaagu gcaggcuaaa gauggacaaa     1440

cuccgauuaa agggagucuc cuacagccug ugcaccgccg ccuucaccuu caccaaaauc     1500

ccagccgaaa cacugcacgg gaccgugaca guagaaguac aauacgccgg cacagaugga     1560

cccugcaaag uacuagcaca aauggccguc gacaugcaaa cacuaacccc agucggccgc     1620

cucauaacgg caaaccccgu gaucacagaa uccacagaaa acucgaaaau gaugcuagaa     1680

cucgacccac ccuucggcga cucauacaua guaaucggag uaggagaaaa gaaaauaacc     1740

caccacuggc acagaagcgg aagcacaaua ggcaaagccu ucgaagcaac cguccgagga     1800

gccaaaagaa uggcagucuu aggcgacacc gcaugggacu ucgggagcgu gggcggagca     1860

cuaaacagcu ugggaaaagg aauccaccaa auauucggag cagcguucaa aagccuguuc     1920

ggaggcauga guugguucag ucagauccuc aucggcacac uccucaugug gcuaggacua     1980

aacaccaaaa acggcagcau cucacugaug ugccucgcgc ucggaggcgu acugauauuc     2040

cugagcaccg ccguauccgc a                                               2061


<210>  2925
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short CDS opt

<400>  2925
accucagucg ggauugucgg acuccuacuc accacagcca uggcagccga ggugacccga       60

cgaggaucag ccuacuacau guaucucgau cgcaacgacg caggcgaagc cauaucguuc      120

cccacaaccc ucggcaugaa caaaugcuac auccaaauca uggacuuagg ccauaugugc      180

gacgccacca ugucauacga augccccaca cuagacgaag gcgucgaacc cgacgacguc      240

gacugcuggu gcaauaccac cuccacaugg gucguauacg gcacaugcca ccacaagaaa      300

ggcgaagccc gccgaucccg cagagccguu accuuaccaa gccacuccac ccgaaaacuc      360

caaacgcgga gccaaaccug gcuagaaagc cgcgaauaca caaaacaccu cauccgcguc      420

gaaaacugga uauuccgcaa cccaggauuc gcccucgccg ccgccgcaau agcauggcug      480

cucggcucca gcacauccca aaaagucauc uaccucguaa ugauccuucu caucgccccc      540

gccuacucca uccgcugcau cggcgucuca aaccgagacu ucgucgaagg caugucaggc      600

ggcaccuggg ucgacgucgu ccuggaacac ggaggcugcg uaaccgucau ggcccaagac      660

aaaccgacag uagacaucga gcuagugacc acaacaguca gcaauauggc cgaagucaga      720

ucuuacugcu augaagcguc caucucugac auggccuccg acucacgcug ucccacgcaa      780

ggggaagccu accuggacaa acaaagcgac acgcaguacg ucuguaaacg cacccuaguc      840

gaccgcggcu ggggcaacgg augcggacua uuuggaaaag gcucacucgu cacuugcgcc      900

aaauucgccu gcucaaagaa gaugaccgga aagucuaucc aaccggaaaa ccuagaauac      960

cgcauaaugc uauccgucca cgggucacaa cacucaggaa ugauagucaa ugacaccgga     1020

cacgaaaccg acgaaaaccg ugcuaaagua gaaaucaccc ccaacucccc uagagccgaa     1080

gcaacccuag ggggauucgg aagucuaggc cuggacugcg aaccacgcac cggccuggac     1140

uucuccgacc ucuacuaccu cacaaugaac aacaagcacu ggcuagucca caaagaaugg     1200

uuccacgaca ucccacuacc uuggcacgcc ggcgccgaca caggcacacc ccacuggaac     1260

aacaaagaag cccucguaga guucaaagac gcucacgcca aacgucaaac cgucgucguc     1320

cuagggagcc aagaaggcgc aguccacacc gcguuggcag gcgcccuaga agcagaaaug     1380

gacggagcaa aaggacgacu cuccucgggc caccuaaaau gccggcuaaa aauggacaaa     1440

cuacgccuca aaggcgucuc cuacagccuc ugcaccgcag ccuucaccuu caccaagauc     1500

ccagccgaaa cccuucacgg caccguaacc guagaagucc aauacgcagg cacagacggc     1560

ccaugcaaag uaccagccca aauggcagua gauaugcaaa cacuaacccc uguuggccgc     1620

cucaucacag ccaaccccgu aaucaccgaa agcaccgaga acucaaaaau gaugcuagag     1680

cucgacccac ccuuuggcga uuccuacauc gucaucgggg uaggcgaaaa gaaaaucacg     1740

caccacuggc acagguccgg aagcacaauc ggcaaagcgu ucgaggcaac uguccgaggc     1800

gccaaacgaa uggcaguccu cggugacacc gccugggauu ucggaagcgu cggcggugcc     1860

cucaauagcc uaggcaaggg cauccaccaa aucuucggcg ccgccuucaa aucccuguuc     1920

ggaggcaugu caugguucuc acagauccua auaggcaccc ugcuaaugug gcuaggccuc     1980

aacaccaaaa acggaucgau cuccuuaaug ugccuagcac ucggcggggu ccuaaucuuc     2040

cuauccacag ccguaucagc c                                               2061


<210>  2926
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short CDS opt

<400>  2926
accucgguag ggaucgucgg ccuacuccua acaacagcca uggccgccga agucacccgc       60

cgaggaagug ccuacuacau guacuuagac cgaaacgacg ccggggaggc cauaucguuc      120

cccacaacac uaggaaugaa caaaugcuac auucagauaa uggaccuagg acacaugugu      180

gacgcaacca ugaguuacga augucccaug cuggacgaag gaguagagcc cgacgacgua      240

gacugcuggu gcaacacaac cagcacuugg guagucuacg gaacguguca ucacaagaaa      300

ggcgaagcac gacgaucccg aagggcugua acgcugccau cacacucgac ccgaaaacuu      360

caaacacgau cgcaaacaug gcuagagucc cgcgaauaca caaaacaccu cauacgcguc      420

gaaaacugga uauuccgcaa ccccggcuuc gcccuggccg cagccgccau cgcauggcuc      480

cucggcagca guaccuccca aaaagucauc uaccucguaa ugauccuacu aaucgcccca      540

gcauacagca uacgaugcau cggaguauca aaccgcgacu ucgucgaagg caugagcggc      600

ggaacauggg uagacguagu auuagaacac ggaggcugcg uaacuguaau ggcgcaagac      660

aaaccgacag uagacaucga gcuagucacc accaccguca guaacauggc agaaguccgg      720

ucauacugcu acgaagccag cauaucagac auggcguccg acagccggug ccccacccag      780

ggcgaagccu accuagacaa acaauccgac acccaauacg ugugcaaaag aacccuagua      840

gacagaggau ggggcaacgg augcggccua uucgggaaag ggucgcucgu aacaugcgca      900

aaauucgccu gcucaaagaa aaugacaggg aaaucaaucc aaccagaaaa ccuagaauac      960

agaaucaugc uaucugucca cggcucccag cacagcggca ugauagucaa cgacacaggc     1020

cacgaaaccg acgaaaacag ggccaaagua gaaaucacac caaacucccc acgcgccgaa     1080

gcaacauuag gcggcuucgg cagccuaggc cucgacugcg agccccgaac gggccuugac     1140

uucuccgacc uauacuaccu caccaugaac aacaaacacu ggcuuguaca caaagagugg     1200

uuccacgaca uaccccuacc cuggcacgca ggcgccgaca caggcacacc ccacuggaac     1260

aacaaagaag cccuggucga guucaaagac gcacacgcaa aacgccaaac aguaguugua     1320

cucggaagcc aggaaggcgc uguacacaca gcccucgcag gagcucugga agccgaaaug     1380

gacggugcaa aaggccgacu cucaagcggc caccugaaau gcagacuaaa aauggacaaa     1440

cucagacuca aaggggugag cuacagccuc ugcacagccg cauucacauu cacaaaaaua     1500

ccagccgaaa cccuucacgg uacagucaca guggaggucc aauacgcagg aacggacgga     1560

ccaugcaaag ugcccgccca aauggcaguc gacaugcaaa cccucacgcc cguagggagg     1620

cucauaacag cuaaccccgu aauaacagag agcacggaaa acagcaaaau gaugcucgag     1680

cucgacccgc ccuuugggga cagcuacaua gucaucggcg uaggagaaaa gaaaaucaca     1740

caucacuggc accgaagcgg cucaacaaua ggcaaagcgu ucgaagcaac aguacgaggc     1800

gccaaaagaa uggcugugcu cggagacacc gccugggacu ucgguuccgu aggcggagca     1860

cucaacucuc ugggcaaagg cauacaccaa aucuucggag cagccuucaa aucccucuuc     1920

ggaggaaugu caugguucag ccaaauccuc auaggaacac ugcucaugug gcucggacua     1980

aacaccaaaa acggcagcau aucccuaaug ugccuggcac uaggaggcgu ccuaauauuc     2040

cucucuaccg ugguaucagc c                                               2061


<210>  2927
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short CDS opt

<400>  2927
accagcguag gcaucgucgg gcuacuacua acaaccgcga uggccgccga agucacacgc       60

agaggguccg ccuauuacau guaccuagac aggaacgacg ccggcgaagc aauaucauuc      120

ccgaccacgc uggggaugaa caaaugcuac auacagauaa uggaccucgg gcacaugugc      180

gacgcaacaa ugaguuacga augccccaug cuggacgaag gcguagaacc ggacgacguc      240

gacugcuggu gcaacacaac gagugcaugg guggucuacg gaaccugcca ccacaagaaa      300

ggcgaagcgc gcagaagccg ccgagcaguc acacuaccaa gccacaguac acguaagcuc      360

caaacaagac cacaaacaug gcucgagucc cgagaguaca caaaacacuu aauacgagua      420

gagaacugga ucuucagaaa cccaggauuc gcacucgcag ccgcagcaau cgcauggcua      480

cuggguucca gcaccagcca aaaagucauc uaccucguaa ugauacuccu aaucgccccg      540

gccuacucca ucaggugcau cggaguaagc aaccgcgauu ucgucgaagg gaugucggga      600

ggaaccuggg uagacgucgu acucgaacac ggcggcugug ucacgguaau ggcccaagac      660

aaaccaacug uagacaucga gcuaguaacc acgaccguau caaacauggc cgaagucaga      720

agcuacugcu acgaagcauc aaucagcgac auggccuccg acagcagaug ccccacccaa      780

ggcgaagccu accuggacaa acaaagcgac acacaauacg uaugcaagag aacacugguc      840

gaccgaggau ggggcaacgg cugcggccuc uucgguaaag ggucgcuagu uacaugcgcc      900

aaauucgcgu gcagcaagaa aaugaccgga aaaagcaucc aaccagaaaa ccucgaauac      960

agaaucaugc uauccguaca cggcagccag cacagcggca ugauaguaaa cgacaccggg     1020

cacgaaacag acgaaaacag ggcaaaaguc gagauaacac ccaacucucc acgagccgaa     1080

gcaacccucg gaggauucgg gagccucgga cuagacugug aaccgaggac cggccuagac     1140

uucagcgacc uguacuacuu aacaaugaau aacaaacacu ggcuuguaca caaagagugg     1200

uuccacgaca ucccccuacc auggcacgcc ggcgcagaca ccggaacgcc gcacuggaac     1260

aauaaagagg cccuaguaga guucaaagac gcccacgcaa aacgacaaac uguagucguc     1320

cuaggcucac aagagggagc aguccauacc gcccuagccg gagcgcucga agcagagaug     1380

gacggagcga aagggcgccu aucaagcgga caccuaaagu gcaggcuaaa aauggauaaa     1440

cugcgccuca agggcgucag cuacucacuc ugcacagcag cauucacauu cacaaaaauc     1500

ccggccgaaa cccuacacgg aaccguaaca guagaaguac aguacgcagg aaccgacgga     1560

cccugcaaag ucccggccca aauggcgguu gacaugcaaa cccuaacgcc cguaggacgc     1620

cugaucacag ccaaccccgu aaucacagaa ucaaccgaaa auucaaagau gaugcuggaa     1680

cuggacccgc cauucggaga cagcuacauc guaauagggg ucggcgaaaa gaaaauaaca     1740

caccacuggc accgauccgg aagcaccauu ggaaaagcau ucgaggccac agucagggga     1800

gcgaaacgaa uggcaguccu cggggauaca gcaugggacu ucggauccgu aggcggagcc     1860

cugaacucac ugggcaaagg aauccaccaa auauucggag cagcguucaa aucacuauuc     1920

ggaggcauga gcugguucuc acaaauacua auaggcacac uacuaaugug gcucggccua     1980

aacacgaaaa acggcagcau cucccuaaug ugccuggccc uaggaggcgu acuaauauuc     2040

cuaucaacag ccguuuccgc c                                               2061


<210>  2928
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short CDS opt

<400>  2928
acaucagucg gaaucgucgg ccucuugcuu acaacagcca uggccgcgga gguaacacgc       60

cggggcagcg ccuacuacau guaccuagac agaaacgacg ccggcgaagc aaucagcuuc      120

cccaccacac ucgguaugaa caaaugcuac auccaaauca uggaccucgg ccacaugugc      180

gacgccacca uguccuacga augccccaug cuagacgaag gcgucgaacc cgacgacgua      240

gacugcuggu gcaacaccac cagcacaugg guggucuacg gaaccugcca ccacaagaaa      300

ggagaagccc gaaggagccg ccgcgcaguc acccuaccca gccacucaac aagaaaacug      360

cagacacgaa gccaaacaug gcuagaaucc cgcgaauaca caaaacaccu aaucagaguc      420

gaaaacugga ucuucagaaa uccaggcuuc gcacucgccg cugcagcaau agcguggcua      480

cucggcagcu caacaucaaa gaaaguaauc uaccuaguaa ugauccuacu gauagcacca      540

gccuacagca ucagaugcau cgggguaucc aacagagacu ucgucgaagg caugagcgga      600

gggacguggg uagacgucgu ccucgaacac ggcggaugcg ucaccguaau ggcacaagac      660

aaaccaacag ucgacauaga acucguaacc acaaccgucu cgaacauggc cgaaguccga      720

agcuacugcu acgaagcgag caucagcgac auggcaucag acucacggug cccaacccaa      780

ggagaagcau accuagacaa acagagcgac acccaauacg ucuguaaaag aacccucguc      840

gaccgcggau ggggcaacgg cugcggccua uucggaaaag gaucacucgu aaccugcgcc      900

aaauucgccu gcuccaagaa aaugacagga aaaaguauuc agcccgaaaa ccuugaguac      960

cgcaucaugc ugucggucca cggaagucaa cacagcggca ugaucguaaa cgacacugga     1020

cacgagaccg acgaaaaucg agcaaaaguc gaaauaacac cgaacucacc ccgcgccgaa     1080

gccacccuug gcggauucgg aucccucggc cuagacugcg aacccagaac cggccuagac     1140

uucucggacc ucuauuaucu cacgaugaac aacaaacacu ggcuugucca caaagaaugg     1200

uuccacgaca uuccacuacc cuggcacgca ggagcagaca ccggcacccc acacuggaac     1260

aacaaagaag cccuagugga guucaaagac gcccacgcca aacgccagac aguaguaguc     1320

cuaggcuccc aagaaggagc cguccacaca gcccuggccg gcgcacuaga agccgaaaug     1380

gacggggcga aagggcgccu uaguagcggg caucuuaaau gccgccuaaa aauggacaaa     1440

cucagacuca aaggagucag cuauucccuc ugcacugcag cauucaccuu cacuaaaauc     1500

ccagccgaaa cccuacacgg caccguaacc guggaaguac aauacgcggg cacagacggc     1560

ccaugcaaag uacccgccca aauggccgua gacaugcaaa cccucacacc agugggcagg     1620

cucauaacag ccaaccccgu caucacggaa ucgaccgaga acaguaaaau gaugcuggaa     1680

uuagacccgc ccuucggaga uagcuacauc guaaucggag uaggagagaa gaaaaucaca     1740

caccacuggc accgaucagg cagcacaauc ggcaaggcuu ucgaggccac cguaagaggc     1800

gcgaaacgca uggcaguacu aggcgacaca gccugggacu ucggcucagu uggaggcgcc     1860

cuaaacagcc uaggcaaagg cauccaccaa aucuucggcg ccgcauuuaa aagccuauuc     1920

ggcggaaugu ccugguucag ccagauccug aucggaacuc uccuaaugug gcugggguua     1980

aacaccaaaa acgggucgau cucacuaaug ugccucgcac uaggcggagu acuaaucuuc     2040

cugagcacag cagucagcgc a                                               2061


<210>  2929
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short CDS opt

<400>  2929
acauccgucg ggaucgucgg ccuccuacug acaaccgcua uggccgccga agucacacgc       60

agaggauccg cuuauuacau guaccucgac cgaaacgacg caggcgaagc gaucaguuuc      120

cccacaaccc ucggaaugaa caaaugcuac auacaaauca uggaccucgg acacaugugc      180

gaugcgacga uguccuacga augccccaug uuagacgaag gcgucgaacc cgacgaugua      240

gacugcuggu gcaacacaac cagcaccugg guaguauacg gcacaugcca ccacaagaaa      300

ggagaggccc gcagaucacg cagagcaguc acacuaccca gucacagcac gagaaaacuc      360

caaacaagga gccagacaug gcucgagagc cgggaauaca caaaacaccu aauacgagua      420

gaaaacugga ucuuccgaaa cccaggauuc gcgcucgccg ccgccgccau cgccuggcuc      480

cucggcagca gcaccagaca gaaaguaaua uaccucguca ugauccuccu aauagcacca      540

gcauauucaa uacgaugcau cgggguaagc aaccgcgacu ucguugaagg gauguccggc      600

ggaacauggg uagaugucgu ccuugaacac ggcggcugcg uaaccguaau ggcccaagac      660

aaacccacgg uagacauaga gcucguuaca acaaccguau ccaacauggc cgaagucagg      720

agcuacugcu acgaggccag cauauccgac auggccagcg acagcagaug ucccacacaa      780

ggagaagcgu accucgacaa acaaagcgac acacaauacg uaugcaaacg aacccuaguc      840

gacagaggcu ggggaaacgg augcggccuc uucggcaagg gcucccucgu aaccugugca      900

aaguucgccu gcuccaagaa gaugaccggc aaaagcauac aacccgaaaa ccuagaauau      960

cgaaucaugc uaagcguaca cgggagccaa cacucaggca ugaucgucaa ugacacaggc     1020

cacgaaacag acgaaaacag ggccaaggua gaaaucaccc ccaacagccc ccgcgccgaa     1080

gccacccugg gcggauucgg cagccuaggc cuagacugcg aacccagaac aggacuagac     1140

uucuccgacc ucuacuaccu cacaaugaac aauaaacauu ggcugguaca caaggaaugg     1200

uuccacgaca uaccccuccc uuggcacgcc ggagcagaca ccggaacucc acacuggaac     1260

aacaaagaag cccuagugga guucaaggac gcacacgcaa agagacaaac ggucguagua     1320

cugggaucac aagaaggcgc aguccacacc gcccucgcgg gagcacuaga agccgaaaug     1380

gacggcgcaa aaggccgacu auccagcggg caccuaaaau gcagacuaaa aauggacaaa     1440

cuccgacuaa aaggagucag uuacagccua ugcaccgccg cauucacguu cacaaaaauc     1500

ccggccgaga cgcugcacgg caccgucacg guugaagugc aauacgccgg cacagacggc     1560

cccugcaagg uucccgccca aauggccgua gacaugcaaa cgcuaacccc agucggccgc     1620

cuuauaacgg ccaaccccgu aaucaccgaa ucaacagaaa acuccaaaau gaugcucgaa     1680

cuagacccac cauucggcga cagcuacauc guaaucggcg ucggcgaaaa gaaaauaacg     1740

caccacuggc aucgcagcgg aagcacaauc ggcaaagcgu ucgaagccac cguacgcgga     1800

gccaaacgca uggccgugcu cggcgacaca gccugggacu ucggaucagu cggcggagcc     1860

cuaaacagcc ucggcaaagg cauacaccaa auauuuggag cggccuucaa aucccuauuc     1920

ggagggauga gcugguucuc ccaaauccug aucggaaccc uccucaugug gcucggacuc     1980

aacacgaaaa acggcagcau aagccuaaug ugccuggccu ugggcggugu acucauauuu     2040

cuaagcaccg ccgucagcgc c                                               2061


<210>  2930
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short CDS opt

<400>  2930
accaguguag ggauaguagg ccuacuccuc acaacggcca uggcagcaga agucacccgu       60

agaggaagcg ccuauuacau guaccuagac cgaaacgaug cgggcgaggc caucagcuuc      120

ccaaccacuc uaggcaugaa uaaaugcuac auacaaauca uggaccucgg acauaugugc      180

gacgcgacaa ugaguuacga augccccaug cuagacgagg gcguagaacc agacgacgug      240

gacugcuggu gcaauacaac cuccacaugg gucgucuacg gaacaugcca ccacaagaaa      300

ggcgaggccc gacgcucgcg ccgcgcugua acauuacccu cgcacuccac ccgaaaacuc      360

caaacacgga gccaaacgug gcucgaaagc agagaauaca caaaacacuu aauaagagua      420

gaaaacugga uauuucgaaa ccccggcuuc gcccuagccg cggcagcgau cgcauggcuc      480

cuuggaagca gcacaagcca gaaagucaua uaccuaguga ugauccuccu caucgcgccc      540

gcauacucaa uccggugcau aggcgucagc aacagagacu ucguagaagg aaugagcggc      600

ggcaccuggg ucgacguagu gcucgagcac ggcggaugcg ucaccguaau ggcacaggac      660

aaaccaaccg ucgacaucga acucgucaca accacggucu caaacauggc cgaagucaga      720

ucguacugcu acgaggccuc caucagcgac auggccagug acuccagaug ccccacgcaa      780

ggggaagccu accucgacaa acaaucagac acacaauacg uaugcaaacg aacacucguc      840

gacaggggau ggggaaacgg cugcggccuc uucgguaaag gaucgcuggu aaccugcgcc      900

aaauucgccu gcagcaagaa aaugaccggg aaaagcaucc aaccugaaaa ccuagaauac      960

cgcauaaugc ucucagucca cgggucccag cacagcggca ugauagucaa cgacacaggc     1020

cacgaaaccg acgaaaacag ggccaaagua gaaaucaccc ccaacagccc acgcgccgaa     1080

gcaacgcuag gcggcuuugg aucccucgga cuagacugug aaccacgaac cggccucgac     1140

uucuccgacc uauacuaucu caccaugaau aacaagcacu ggcucgugca caaagaaugg     1200

uuccacgaca ucccacuccc auggcacgcc ggcgccgaca ccggaacgcc acauuggaac     1260

aacaaagaag cccuagugga guucaaagac gcacacgcaa aaaggcaaac agucgucgua     1320

cucggaacuc aagaaggagc aguccacaca gcgcucgcag gagcacucga agcagaaaug     1380

gacggggcaa agggcagacu auccagcgga caccucaagu gccggcucaa gauggacaaa     1440

cuacgccuaa aaggcguaag cuacagccua ugcaccgcag ccuucaccuu caccaagaua     1500

cccgcagaaa cccuacacgg cacagucaca guggaagucc aauacgccgg caccgacgga     1560

cccugcaaag uccccgccca aauggcaguc gacaugcaga cgcucacacc cguaggacgu     1620

uuaaucacag cgaaccccgu aauaaccgag ucaaccgaaa acagcaaaau gaugcuagaa     1680

cucgacccac cauucggcga caguuacaua gucaucggcg uaggcgagaa aaagauaacc     1740

caccacuggc accgaagcgg gagcacaaua ggcaaagccu ucgaagcaac cguccgagga     1800

gccaaaagaa uggccguccu aggagacacc gccugggauu ucgggagcgu cggcggggca     1860

cucaacagcc ucggaaaagg cauacaccaa auauucgggg cagcauucaa gagcuuguuu     1920

ggagggaugu caugguucuc ccagauccuc aucggcaccc uacucaugug gcuaggacua     1980

aacaccaaaa acgggucgau cucacucaug ugccuagccc ucgggggcgu acuuaucuuc     2040

cuaucaacag caguaagcgc a                                               2061


<210>  2931
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short CDS opt

<400>  2931
acgagcgucg gcaucguagg acuacuccuc acuaccgcca uggcagccga agucacaaga       60

cgaggauccg ccuacuacau guaccuagac cgcaacgacg caggcgaagc aauauccuuc      120

ccaaccacgc uggguaugaa caaaugcuac auacaaauaa uggaccucgg acacaugugc      180

gacgcaacaa ugucguacga augcccgaug cuagacgaag gaguagagcc agacgacguc      240

gacugcuggu gcaacacaac cuccacgugg gucguguacg gcacaugcca ccacaaaaag      300

ggagaggccc gaagaagccg acgagccgua acccuccccu cacacucgac ccgcaaacuu      360

cagacacgca gccaaacaug gcuagaaagc agagaauaca ccaagcaccu gaucagggua      420

gaaaauugga uauuccgcaa ccccggauuu gcccuagcag ccgcagccau cgcauggcuc      480

cugggaucca gcacaucaca gaaaguaaua uaccuuguaa ugauccuacu aaucgcacca      540

gccuacuccc uccggugcau aggcgucagc aaccgcgacu ucgucgaagg caugucaggc      600

gggaccuggg uagacguagu acucgaacau gggggaugcg ucaccguaau ggcacaggac      660

aaaccaaccg ucgacaucga gcugguaacc acaacaguaa gcaacauggc agaagugcgc      720

ucauacugcu acgaagcauc caucagcgac auggccagcg acucccgcug cccaacacaa      780

ggcgaagccu accuagacaa acaaagcgac acccaauacg ucugcaagag aacccucgua      840

gaccgaggcu gggguaacgg cugcggacuc uucggcaaag gcucacucgu gacuugcgcc      900

aaguucgcau gcagcaagaa aaugacaggc aaaagcauac aaccagaaaa ccuagaauac      960

agaaucaugc ucagcguuca cggaucccaa cacuccggga ugaucgucaa cgacacaggc     1020

cacgaaacag acgaaaaccg cgcaaaaguc gaaaucacac caaacagccc acgcgcagaa     1080

gcaacacuug gcggauucgg cagccucggg cuagacugcg aaccccgaac gggccuggac     1140

uucagcgacc uauacuaccu aaccaugaac aacaaacacu ggcuagucca caaagaaugg     1200

uuccacgaca ucccccuccc auggcacgcc ggagccgaca caggaacacc ccacuggaac     1260

aauaaagaag cccuaguaga guucaaagac gcccacgcca aacgccaaac aguaguaguu     1320

cuagggagcc aagaaggagc aguccacaca gcgcucgcag gcgcccugga ggcagaaaug     1380

gacggagcca aaggacggcu cuccagcgga caccuuaagu gccggcuaaa aauggacaaa     1440

cucagacuca aaggcguauc uuacaguuua uguaccgccg ccuuuacauu caccaagauc     1500

cccgcugaaa cacuucacgg gaccgucaca gucgaagucc aauacgcggg uaccgacgga     1560

ccaugcaagg uccccgcgca aauggcagua gacaugcaga cauuaacacc cguaggccgg     1620

cuuaucacag caaacccagu aaucacagaa ucaacagaaa acucaaaaau gaugcucgag     1680

cuagacccgc ccuucgggga cucauacauc gucaucggag ucggagagaa aaagaucaca     1740

caccacuggc accgcagcgg cagcacgauc ggaaaagcgu ucgaagccac ugugcgcgga     1800

gcgaaaagaa uggcaguacu gggagacacu gcgugggacu ucggcucagu cggaggggca     1860

cuaaacagcc ugggcaaagg aauacaccaa auauucggcg cggcauucaa aucccucuuc     1920

ggaggaauga guugguucuc acaaauccug aucggcaccc uacucaugug gcugggucuc     1980

aacaccaaaa acgggucaau aagccucaug ugccucgcac uaggcggagu acuaaucuuc     2040

cucaguacag caguaagcgc c                                               2061


<210>  2932
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short CDS opt

<400>  2932
accuccgucg ggauaguagg ccuacuacuc accaccgcua uggcagcaga aguaacccga       60

aggggcagcg cauauuacau guaccuagac agaaacgacg ccggcgaagc gaucaguuuc      120

ccgacaacgc ugggaaugaa caaaugcuac auacaaauca uggaccucgg acacaugugu      180

gacgccacca ugucauacga augcccgaug cucgacgagg gagucgaacc cgaugaugua      240

gacugcuggu gcaacacaac aagcacuugg gucgucuacg ggaccugcca ccacaagaaa      300

ggcgaagcac gucgcagccg cagagcugua acccucccau cgcacagcac acgcaaacuc      360

caaacccgcu cccaaacgug gcuggaaucc cgcgaguaca ccaaacacuu aaucagaauc      420

gaaaauugga ucuuccgcaa ccccggcuuc gcacucgcgg cagccgccau agcguggcuc      480

cuaggcucau ccacgaguca aaaaguaaua uaccucguca ugauccuacu aauagccccc      540

gccuacucca uacgcugcau aggcgucagc aaccgagacu ucguagaagg aaugucugga      600

ggcaccuggg ucgacguagu acuggaacac ggcggcugcg ucacggugau ggcacaagac      660

aaaccgaccg uagacaucga acucgucacc accacaguaa gcaacauggc ugagguccgc      720

ucguacugcu acgaggcaag cauaagcgac auggcaucag acuccaggug ccccacccaa      780

ggagaagccu accucgacaa acaaagcgac acccaauaug uaugcaaaag gacccuaguc      840

gaccgcgggu ggggaaacgg cugcggacua uucggcaaag gaagccucgu uaccugcgca      900

aaauucgcau gcuccaagaa aaugaccggc aaaucuauac aaccagaaaa ccucgaauac      960

agaauaaugc uaucagucca cggcucacaa cacagcggca ugaucgucaa ugacaccggc     1020

cacgaaaccg acgaaaacag agcaaaagua gaaaucaccc ccaacagccc acgagccgag     1080

gccacccucg gcggcuucgg aucgcugggg cuagacugcg aacccagaac aggccucgac     1140

uucucugacc uauacuaccu aacgaugaac aacaaacacu ggcucgucca caaagaaugg     1200

uuccacgaca ucccacuacc auggcacgca ggcgcagaca caggaacacc ccacuggaau     1260

aauaaagagg cacuugucga guucaaagac gcacacgcca aaagacaaac cgucguaguc     1320

cucggcagcc aagaaggugc uguacacacc gcacucgccg gcgcccucga agcagaaaug     1380

gacggcgcaa aaggccgccu aucaucaggc caccuaaaau gccgccucaa aauggacaaa     1440

cuccgacuaa aaggcgucag cuacucgcuc ugcaccgcgg ccuucaccuu caccaaaauc     1500

cccgcagaaa cccuacacgg aaccgugacc gucgaagucc aauacgcagg aaccgacgga     1560

ccaugcaaag ucccagcaca aauggccguc gacaugcaga cacuaacacc aguaggcaga     1620

cucauaacug ccaacccagu aaucacagaa uccaccgaaa acaguaaaau gaugcucgaa     1680

cuagacccgc cauucggcga cagcuacauc gucaucggcg ucggggaaaa gaaaaucaca     1740

caucacuggc accgcucagg auccacaauc ggcaaagccu ucgaagccac aguccgaggg     1800

gcuaaaagaa uggcaguccu cggcgacacc gcaugggacu ucggcagcgu aggcggcgcc     1860

cuaaacucac ucggaaaagg cauucaccaa auauucggcg cagcauucaa aucacuuuuc     1920

ggaggaauga gcugguucuc acaaauacua aucggaacac uacuaaugug gcucggacuc     1980

aacaccaaaa acggguccau aagccugaug ugucuagccc uaggaggagu acucaucuuc     2040

cuaagcaccg cagucuccgc a                                               2061


<210>  2933
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short CDS opt

<400>  2933
acaucagugg gaauaguagg acuccuacua acgaccgcaa uggccgcaga aguuacccga       60

agaggcagcg cauacuauau guaccucgac cgcaacgacg ccggcgaagc caucucauuc      120

ccaaccacac ucgggaugaa caaaugcuac auccaaauaa uggaccuagg acacaugugc      180

gacgcgacca ugucauacga gugucccaug cuugacgaag gcguagaacc ggacgacgua      240

gacugcuggu gcaacaccac gagcaccugg gugguauacg gcaccugcca ccacaaaaaa      300

ggcgaggcga gaaggagcag acgagccguc acccuuccca gccauucaac ccgaaaacua      360

caaacaagaa gucaaacgug gcuagaauca cgcgaauaca caaaacaccu gauccgcgua      420

gaaaacugga uccuaaggaa cccaggauuc gcacuggccg ccgcggccau cgccuggcuc      480

cuagggagca gcacauccca aaaaguaauc uaccuaguca ugauccuccu gauagcgccc      540

gcguacagca ucagauguau cggcgucucc aaccgagacu ucguagaagg aaugucuggg      600

gguacauggg ucgacgucgu ccuagaacac ggcggcugcg ucacaguaau ggcacaagac      660

aaaccaacgg uagacauaga acucgugacc accaccgucu caaacauggc agaaguacga      720

ucguacugcu acgaggcguc cauaucagac auggccagcg acagccgcug cccaacccag      780

ggagaggcau accucgacaa acaauccgac acccaauacg ugugcaaaag gacacuaguc      840

gaccgggggu gggggaacgg cugcggccuc uuuggaaaag gcagccucgu cacaugcgcc      900

aaauucgccu gcuccaaaaa aaugacaggg aaaagcaucc aaccagaaaa ccuagaauac      960

cgaaucaugc ucuccgugca cggcucacaa cacuccggga ugauaguaaa cgacacagga     1020

cacgagaccg acgaaaaccg agccaaaguu gaaauaaccc ccaacagccc cagagcugaa     1080

gccacacuug gaggauucgg aucccuaggc cucgacugcg aaccacgcac gggccucgac     1140

uuuagcgacc uauacuaccu aaccaugaac aacaaacacu ggcuggucca caaagaaugg     1200

uuccacgaca ucccgcugcc auggcacgcc ggcgccgaca cgggaacccc ccacuggaac     1260

aacaaagagg cacucgucga auuuaaagac gcacacgcca aacgacaaac cgucguugua     1320

cuaggcucac aagagggcgc gguccauaca gcacuagcag gcgcccugga ggccgaaaug     1380

gacggagcca aaggccgacu cuccagcgga caucuaaaau gccgacugaa gauggauaaa     1440

cuacgacuca aaggagucuc auacucacua uguaccgcag cauuuacauu cacaaaaaua     1500

cccgcagaaa cacuacacgg aaccguaacc gucgaagucc aguacgccgg cacggacggc     1560

cccugcaaag uccccgccca aauggccguc gacaugcaaa cacugacccc agucggccgc     1620

cucauaaccg caaacccagu caucacugaa uccaccgaga acagcaaaau gaugcucgag     1680

cucgaccccc ccuucggcga cagcuacauc gucaucggca ucggggaaaa gaaaauaaca     1740

caccacuggc accgauccgg cagcaccauc ggcaaagccu ucgaggcaac aguccgcgga     1800

gcaaaacgca uggcaguccu cggagacacc gccugggacu ucggcagcgu cggaggagcc     1860

cucaacucuc ugggcaaagg gauacaccaa aucuucggag cagcauucaa aagccuauuc     1920

ggcggaauga gcugguucuc ccaaauacuc auaggaaccc uccucaugug gcucggacua     1980

aacaccaaaa acggauccau aagccuaaug ugccuagcuc ucggaggcgu gcuaauauuc     2040

uuaaguaccg ccgucagcgc c                                               2061


<210>  2934
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short CDS opt

<400>  2934
accuccguag gaaucgucgg ccuccugcua accaccgcca uggcagccga agucacacgc       60

cgcggcucag cauacuacau guaucucgac agaaaugacg ccggugaagc cauaucauuc      120

cccaccacac ugggcaugaa uaagugcuac auccaaauca uggaccuggg ccacaugugc      180

gacgccacaa uguccuacga augucccaug cucgacgaag gcgucgagcc cgacgacgua      240

gacugcuggu gcaacaccac uagcacgugg gucgucuacg gaaccugcca ccacaagaaa      300

ggcgaagccc gccgaucaag aagggcaguc acccuucccu cacacagcac ccgaaaauua      360

caaacaagaa gccaaacaug gcucgaaagc cgcgaauaca caaaacaccu uauccgcgua      420

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcua      480

cuuggcucau ccaccagcca aaaagucauc uaccuaguca ugauccuacu caucgcaccc      540

gccuacucca uccgaugcau cggcguaucc aaccgcgacu ucgucgaagg aaugaguggc      600

ggcaccuggg uagacguugu ccucgaacac ggcggaugcg uaaccgucau ggcccaagac      660

aaaccgaccg ucgacauaga acucgucuca accaccguaa gcaacauggc agaaguaaga      720

ucauacugcu acgaagcaac cauaagcgac auugcgucgg acucccgcug cccaacucag      780

ggcgaagccu accuagacaa acaaucagac acccaauacg ugugcaaacg aacccucgug      840

gauagaggcu ggggcaaugg gugcgggcuu uucgggaaag gcucccuggu cacaugcgcc      900

aaauucgccu guagcaagaa aaugacaggc aaauccaucc aaccggaaaa ccucgaauac      960

cgcaucaugc uaucaguaca cggcagccaa cacagcggca ugauuguaaa cgacacagga     1020

cacgaaaccg augaaaaccg agccaaaguc gaaauuaccc ccaacucacc ccgcgccgag     1080

gcaacccuug gaggcuucgg cucccucgga cuggacugcg aaccaagaac aggccucgac     1140

uuuuccgacc ucuacuaccu aaccaugaac aacaaacacu ggcucguaca caaggaaugg     1200

uuccacgaca ucccccuucc cuggcacgcg ggagcggaca ccggcacccc acacuggaac     1260

aacaaagaag cgcuggucga guucaaagac gcccaugcca aacgccaaac agccgucgug     1320

cucgggagcc aagaaggagc aguucacacc gcccuagccg gcgcccugga agcagaaaug     1380

gacggcgcca aagggcgacu cuccuccggc caccucaaau gccgacuaaa gauggacaaa     1440

uuacgccuaa aaggagucuc cuauagccua ugcacagccg cauucacauu cacaaagaua     1500

cccgcagaaa cccuccacgg caccguaacc gucgaaguac aauacgcagg aacggacgga     1560

ccaugcaaag uacccgccca aauggcaguc gacaugcaaa cccuaacccc cgucggacga     1620

cucauaaccg ccaacccagu caucacagaa agcaccgaaa acucaaaaau gaugcucgaa     1680

cucgacccac ccuucggcga cucauacauc gucaucggcg ucggagaaaa gaaaauaacc     1740

caccacuggc accgcuccgg cagcacgauc gggaaagcau ucgaagccac aguccgagga     1800

gccaagcgaa uggcaguccu aggcgauaca gccugggacu ucggcucagu agguggcgcc     1860

cuaaacucac ucggaaaggg aauccaccaa auauuuggcg ccgcauucaa aucccuauuc     1920

ggcggcaugu ccugguucag ccagauauua auaggaacac uacucaugug gcuagggcuc     1980

aauaccaaaa acggcuccau cucgcuaaug ugccuagccc ucgguggcgu acucaucuuc     2040

cucagcaccg cagucuccgc c                                               2061


<210>  2935
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short CDS opt

<400>  2935
acaagcguug gaaucguagg acuacuacug accaccgcca uggcugccga agucacacgc       60

cgaggcucag ccuacuacau guaccucgac cgaaacgacg ccggugaagc aauaucauuc      120

ccaaccacac ucggcaugaa caaaugcuac auccaaauca uggaccucgg acacaugugc      180

gacgccacca ugagcuacga augucccaug cuagaugagg gcgucgaacc agacgauguc      240

gacugcuggu gcaacaccac cuccaccugg gucguguacg guaccugcca ccacaagaaa      300

ggcgaagccc gacgaucacg acgcgcaguc acacucccaa gccacagcac caggaaacuc      360

caaacacgca gccagacaug gcucgaaagc cgcgaauaua caaaacaccu aauaagagua      420

gaaaacugga ucuuccgaaa cccaggcuuc gcccucgcag ccgccgcgau cgcauggcua      480

cucgggagcu caaccuccca aaaagucauc uaccuaguua ugauacuacu aauagcaccc      540

gcuuacucca uccgaugcau aggcgucuca aaccgcgacu ucgucgaagg aaugagcgga      600

ggcaccuggg uagacguagu ccucgagcac ggagguugug uaaccgucau ggcucaagac      660

aaaccaacgg ucgacauaga gcuaguaaca acaaccgucu caaacauggc agaaguccgg      720

agcuacugcu acgaagccag caucuccgac auggccucug auucccgcug cccaacacaa      780

ggcgaggcuu accuagacaa acagucagac acccaguaug uuugcaaacg gacacucguc      840

gaccgagggu ggggcaacgg augcggccuc uucggcaaag gcucccucgu aacaugcgca      900

aaauucgcuu guucaaagaa aaugacaggc aaaucaaucc aaccggagaa ccucgaauac      960

cgcaucaugc ucagcgucca cgggagccaa cacuccggaa ugauagucaa cgacaccgga     1020

cacgaaaccg acgaaaaccg agcaaaagug gaaaucacgc cuaacagccc ccgggccgaa     1080

gcaacccuag gaggcuucgg gucccugggg cuugacugcg aaccucguac cggccuagac     1140

uucagcgacc ucuacuaccu aaccaugaac aacaaacauu ggcuaguaca caaagagugg     1200

uuccacgaca uccccuuacc cuggcacgcc ggagcagaca ccggcacacc ccacuggaac     1260

aacaaagaag cccucguuga guucaaagac gcacacgcaa aacgccaaac cguagugguu     1320

cuaggauccc aagaaggagc cguccacacc gcccucgccg gagcacucga agccgaaaug     1380

gacggcgcaa aaggcaggcu cagcagcgga caccucaaau gccgacuaaa aauggacaaa     1440

cuccgacuca aaggcguguc cuacucccug ugcacagccg ccuucaccuu caccaaaauc     1500

cccgccgaaa cacuccacgg cacgguaaca guagaacuac aauacgccgg gacagaugga     1560

ccaugcaaag uccccgcaca aauggcagua gauaugcaaa cacuaacccc cguaggccgc     1620

cuuaucaccg caaaccccgu caucaccgaa uccacagaaa acuccaaaau gaugcuugaa     1680

cucgacccac cauucggcga uucauacauc gucauaggcg ucggagaaaa gaaaaucacg     1740

caccacuggc acaggagcgg cuccaccaua ggcaaagcgu uugaagccac cgugcgcggc     1800

gccaaacgaa uggcaguccu cggagacaca gccugggacu ucggcuccgu uggcggggca     1860

cuaaacucac ucggcaaagg cauccaccaa aucuuuggag ccgcguucaa aucacucuuc     1920

gguggcaugu cuugguucuc ucagauccua aucggcacuc uucucaugug gcuaggacuc     1980

aauaccaaaa acggcucgau cagccucaug ugccucgcac ucggcggcgu acucauuuuc     2040

cucagcacag ccgucucagc c                                               2061


<210>  2936
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short CDS opt

<400>  2936
acauccguag gaaucgucgg acuccugcuc acaaccgcca uggccgccga agucacaaga       60

cgaggcuccg cauacuacau guaccucgac cgcaacgacg ccggcgaggc aaucagcuuc      120

ccaaccaccc ucggcaugaa caaaugcuac auacaaauaa uggaucuagg acacaugugc      180

gacgccacga ugagcuacga augccccaug cuggacgaag gaguggagcc agacgacguc      240

gacugcuggu gcaacaccac aagcacaugg guuguauacg gaaccugcca ccacaagaaa      300

ggcgaggccc gccgcagcag acgagcagua acucuaccau cccacuccac acgcaaacuc      360

caaacucgau cccagaccug gcucgaaagc cgagaauaca caaaacaucu cauacgcguc      420

gaaaacugga uauucagaaa cccaggguuc gcacucgccg ccgccgccau cgccuggcuc      480

cuaggcagca gcacauccca aaaagucauc uaccuaguaa ugaucuuauu aauagccccc      540

gcguacucca uacggugcau cggaguauca aaccgagacu ucguagaagg aaugagcggg      600

ggcacguggg ucgacguugu ccucgaacac ggcggcugcg uaaccguaau ggcccaagac      660

aaaccaacag uagacauaga acucgucacc acaaccguca gcaacauggc cgaagugcgc      720

ucauacugcu acgaagccuc cauuuccgac auggccucug acaguaggug cccaacgcag      780

ggagaagcau accuagacaa acaaagcgac acccaauacg ucugcaagcg cacucuaguc      840

gaccgaggau ggggaaacgg gugcggucua uucggaaaag gcagucuugu aaccugcgca      900

aaauucgcuu gcuccaagaa aaugacaggc aaaagcauac aacccgaaaa ccucgaauac      960

cgcaucaugc ucuccgucca cggcucacaa cacucaggga ugaucgucaa ugacacaggc     1020

caugaaaccg acgaaaaccg ggccaaaguc gaaaucaccc ccaacagccc cagagccgaa     1080

gcaacccuag gaggcuucgg cagccuaggc cucgacugcg aaccucgcac cggucuggac     1140

uucuccgauc ucuacuaccu caccaugaac aacaaacauu ggcucgucca caaagaaugg     1200

uuccacgaca ucccccuccc auggcacgcc ggagccgaca caggaacacc acauuggaac     1260

aacaaagaag cacuggucga guucaaagau gcccaugcca agagacagac cgucgugguc     1320

cucggcucac aagaaggcgc cguacacacc gcacucgccg gagcgcucga agccgaaaug     1380

gacggagcca aagguaggcu aagcuccgga caccugaaau gcagacuuaa aauggacaag     1440

uuacgccuca aaggcgucuc auacucccuu ugcacagccg cauucacuuu cacaaaaauc     1500

cccgccgaaa ccuuacacgg gacaguaacu guggaagugc aauacgccgg caccgacgga     1560

ccgugcaagg uccccgcgca aauggcaguc gacaugcaaa cacuaacacc cguaggccgc     1620

cugaucacag caaacccugu uaucacagaa ucaauagaaa acuccaaaau gaugcuugaa     1680

cucgacccac cguucggaga cagcuacauc guaauaggcg ucggcgaaaa gaaaauuacc     1740

caccacuggc accgcucagg cagcacaauc ggaaaagccu ucgaagccac aguccggggc     1800

gccaaacgaa uggccguccu cggcgacacc gcaugggacu ucggaucagu aggaggcgca     1860

cucaacaguc uugggaaagg aauccaccaa auauucggag ccgccuucaa gagccucuuc     1920

ggaggcaugu ccugguuuag ccaaauacua auaggcacac uccuaaugug gcuaggccuc     1980

aacaccaaga acggcagcau cagccucaug ugccucgccc ucggaggagu acuaauauuc     2040

cuauccaccg ccgucagcgc c                                               2061


<210>  2937
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short CDS opt

<400>  2937
acaaguguag gaaucguggg ucuccuccug acaaccgcaa uggccgcaga aguaacacgg       60

cggggguccg cauacuacau guaccucgac cggaacgacg caggcgaggc caucucuuuc      120

cccaccaccc uaggcaugaa caaaugcuac auccaaauaa uggaccuagg acacaugugc      180

gacgcaacca uguccuacga augccccaug cuagaugaag gagucgaacc agacgacgug      240

gacugcuggu gcaacaccac caguaccugg gucgucuacg gaacaugcca ccacaagaaa      300

ggggaggcuc gcagaagccg ccgagcuguc acacucccaa gccacagcac cagaaaacuc      360

caaacacgau cccaaacaug gcuagaaucg cgcgaguaca ccaaacaccu cauccgcgug      420

gaaaacugga ucuuccgaaa cccaggcuuc gcccucgcag cggcagcaau agcauggcug      480

uuaggauccu ccacaagcca aaaaguaaua uaccugguaa ugauccuacu caucgcaccc      540

gcauacucca uccgaugcau aggaguaucc aaccgggacu ucgucgaagg aaugagcgga      600

ggaaccuggg ucgacguggu ccuagaacac ggcggaugcg ucaccgucau ggcacaagac      660

aaacccacag uggacaucga gcuaguaacc accaccgugu caaacauggc ugaaguuaga      720

uccuacugcu acgaagcaag caucagcgac auggcgagug acagcagaug ccccacacaa      780

ggcgaagcau accucgacaa acaauccgau acccaauacg uaugcaaacg cacacucgua      840

gaccgcgguu ggggcaacgg cugcggauua uucgggaaag ggagccucgu uaccugcgca      900

aaauucgccu gcagcaaaaa gaugacaggc aaaagcaucc aacccgagaa ucuagaauac      960

agaauaaugc ucuccguaca cggaucccaa cacucaggga ugauaguuaa ugacacaggc     1020

cacgaaacag acgaaaaccg agccaaaguu gaaauaaccc cgaacucacc aagagccgaa     1080

gccacccuag gaggcuucgg aucucucgga cucgacugcg aaccccgcac aggacuagac     1140

uucuccgacc uauacuaucu cacuaugaac aacaagcacu ggcucguaca uaaagaaugg     1200

uuccacgaca ucccacuacc cuggcacgcg ggcgcagaca cagggacgcc ccacuggaac     1260

aauaaagaag cgcucguaga guucaaggau gcucacgcca agcgccaaac agucguagua     1320

cuaggcuccc aagaaggcgc cguccacaca gcccuagcag gagcccugga agccgaaaug     1380

gacggaguaa agggcagacu cucuagcggg caccuuaaau gccgacugaa aauggacaaa     1440

cucagguuaa agggcguauc cuauucacug ugcaccgcgg cauucacauu caccaaaauc     1500

ccggccgaaa cacuccacgg cacaguaacg gucgaagucc aauacgccgg cacggacgga     1560

cccugcaaag uacccgccca aauggcaguc gacaugcaaa cccucacacc cgucggaagg     1620

cuaauaacag ccaaccccgu caucaccgaa agcaccgaaa acagcaaaau gaugcucgaa     1680

cuagacccac ccuucggcga cucauacaua gugaucggcg ucggcgaaaa gaaaaucacg     1740

caccacuggc acagaucagg aucgacaaua ggaaaagccu ucgaagcgac aguccgcgga     1800

gcaaaacgaa uggccguauu aggggacaca gcaugggacu ucggcuccgu cggcggcgca     1860

cucaacuccc ucgguaaagg aauacaccaa auauucggug cagcuuucaa aucacuauuc     1920

ggcggcaugu ccugguucag ccaaauucuc aucggaacac uccucaugug gcucggccuu     1980

aacacuaaaa auggcucaau cucguucaug ugccucgccc uaggcggagu ccugaucuuc     2040

cucuccaccg cgguauccgc c                                               2061


<210>  2938
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short CDS opt

<400>  2938
acaagcguag gaauagucgg acuacuacuc acaacggcaa uggcagccga agucacccga       60

agagggagcg ccuauuacau guaccuagac agaaacgacg caggagaagc aaucucauuc      120

cccacgacac ucgggaugaa caaaugcuac auacaaauaa uggaccuagg acauaugugc      180

gacgcgacaa ugucauacga augcccaaug cucgacgaag gaguggaacc cgacgacgua      240

gacugcuggu gcaacaccac auccaccugg gugguauaug gcacaugcca ccacaagaaa      300

ggcgaagcac gccgaagccg ccgagcaguc acacucccaa gccacagcac ccgcaaacuc      360

cagacaagaa gccaaacaug gcuagaaucc cgagaauaca cuaaacaccu aauacgggua      420

gaaaacugga uauucagaaa cccaggauuc gcacuugcag ccgcagccau cgcuuggcuc      480

cuaggaagcu ccacguccca aaaagugauc uaucucguca ugauacuacu gaucgccccc      540

gccuacagca uacgcugcau aggcguaagc aaccgagauu ucguggaagg aaugagcggc      600

ggcaccuggg uagacgucgu ucuagaacac ggaggcugcg uaaccguaau ggcccaagac      660

aaacccaccg uggacaucga acugguuaca accaccguau ccaacauggc cgagguuaga      720

agcuacugcu acgaagcguc caucucagac auggccucag acucacgcug ccccacacaa      780

ggagaagcau accucgacaa acaaucagau acgcaguacg ucugcaaaag aacacuggug      840

gaccgcggcu ggggaaacgg augcggccug uucgguaaag gcagccucgu cacaugcgca      900

aaauucgcgu gcucgaaaaa gaugacaggc aaaagcauac aacccgaaaa ccuagaauac      960

cgaaucaugc uauccgucca cggcucacaa cacucuggaa ugaucgucaa cgacacgggc     1020

caugaaacgg acgaaaacag agccaaaguc gagauaacac ccaacagccc cagggccgaa     1080

gcgacacucg gaggcuucgg uagccuggga cuagacugcg aaccgcgcac aggccuggac     1140

uucagcgacc uuuacuaccu gaccaugaau aacaaacacu ggcucgucca caaggaaugg     1200

uuccacgaca ucccccugcc auggcaugcc ggcgcagaca ccggcacccc acacuggaac     1260

aacaaagaag cccuaguaga guucaaagac gcacacgcaa aacgccagac aguaguaguc     1320

cugggcucac aggaaggcgc agugcacacc gcacuggcag gcgcgcugga agccgagaug     1380

gacggcgcca aagguaaacu aucuucgggc caccucaaau gccgucuaaa aauggacaaa     1440

cuaagacuaa aaggggucuc cuacagccuc ugcaccgcag ccuucaccuu uacaaaaaua     1500

ccagcagaaa cacuccacgg gacaguaacc gucgaagugc aguacgccgg cacagacggc     1560

cccugcaagg uccccgcaca gauggcaguc gacaugcaga cacucacccc aguaggacgc     1620

cucauaaccg caaaccccgu caucaccgaa agcaccgaga acaguaaaau gaugcucgaa     1680

uuggacccac cauucggaga cagcuacauc guaauaggag uuggcgaaaa gaaaaucaca     1740

caccauuggc acagaagcgg gagcaccaua ggcaaagcau ucgaagccac gguccgaggc     1800

gcaaaacgga uggcagugcu aggcgacacc gcaugggacu uuggaagcgu gggcggagca     1860

cugaacaguc uaggaaaagg cauacaccag auauucggag cagcguucaa aagccuguuu     1920

ggcggaaugu caugguucuc gcaaauccuc auaggcaccc uacuuaugug gcuagggcuc     1980

aacaccaaaa auggaagcau cagccuaaug ugccuagcac uagggggcgu ccuaauauuc     2040

cucagcaccg ccguaagcgc a                                               2061


<210>  2939
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short CDS opt

<400>  2939
acgagcgucg gaaucgucgg acuccuccua accaccgcaa uggcggccga agucacccgc       60

cgaggcagcg cauacuacau guaccuagac cgaaaagacg ccggagaagc gaucagcuuc      120

cccaccacac uaggaaugaa caaauguuac auacaaauca uggaucuggg acacaugugc      180

gacgccacca ugagcuacga augccccaug cucgacgaag gcguagaacc ggacgacgua      240

gacugcuggu gcaacaccac cagcaccugg gucguauacg gaaccugcca ccacaagaaa      300

ggagaagcac ggcgaagccg acgcgccguc acacuccccu cacacagcac ccgcaaacuc      360

caaacccgau cccaaaccug gcucgagagc agagaauaca cgaaacacuu aauacgagua      420

gagaacugga uauuccgcaa ccccggauuc gcacuagcag cagccgccau cgccuggcua      480

cucggcucau ccacaucaca aaaagucauc uaccugguca ugauacuacu aauagcaccc      540

gccuacagca uacgcugcau aggaguaagc aaccgcgacu ucgucgaagg aauguccggc      600

ggcaccuggg uagacguagu ccuggagcac ggcggcugug ucaccguaau ggcacaagac      660

aaacccaccg uagacaucga gcuaguaaca accaccguca guaacauggc agaaguacga      720

uccuacugcu acgaagcaag caucuccgac auggcgucag acucccggug ucccacgcaa      780

ggagaagcau accucgacaa gcaaagcgac acacaauacg ucugcaaacg aacccucguc      840

gaccgaggau ggggcaacgg uugcggccug uucggaaaag gaucccucgu gaccugcgca      900

aaauucgcau gcagcaagaa aaugaccggc aagagcaucc aacccgaaaa ccuagaauac      960

cgcaucaugc ugucagucca cggcagccaa cacagcggga ugauagucaa cgacacuggc     1020

cacgaaaccg acgaaaacag agcaaaagug gagauaaccc caaacagccc ccgagccgag     1080

gcaacacucg gaggauucgg gagccucggc cucgacugcg aacccaggac cggacucgac     1140

uucagcgacc ucuacuaccu gaccaugaac aacaaacacu ggcucgucca caaagaaugg     1200

uuccacgaca uaccucugcc uuggcacgca ggcgccgaca cgggaacucc acacuggaac     1260

aacaaagaag cccucguaga guucaaggac gcccacgcga aaaggcaaac gguaguugua     1320

cuaggcaguc aagaaggcgc aguacacacc gcccuagccg gcgcccuaga agccgaaaug     1380

gacggcgcca aaggccgucu auccagcgga cacuuaaagu gccgacuaaa aauggacaaa     1440

cuucgccuca aaggggucuc auacagucuc ugcaccgcug cguuuaccuu caccaaaauc     1500

cccgcagaaa cacuccacgg aacugucaca gucgaagugc aauacgccgg gacagacggc     1560

cccugcaaag uaccagcaca gauggccguc gacaugcaga ccuuaacacc ugucggaaga     1620

cucaucacag ccaaccccgu cauaaccgaa uccacagaaa acagcaaaau gauguuagaa     1680

cucgacccuc ccuucggaga cucauacaua guaaucggcg ugggcgaaaa gaaaaucacc     1740

caccacuggc acagauccgg auccaccauc ggaaaagccu ucgaagccac cguccgaggc     1800

gccaaacgca uggccguauu gggagacaca gcaugggacu ucggguccgu aggaggcgcc     1860

uuaaacucgc uagggaaagg cauccaccaa aucuucggag ccgccuucaa aagccucuuc     1920

gguggcauga gcugguucuc ccaaauccuc aucggcacgc uccuaaugug gcuaggucug     1980

aacaccaaga acggcucaau aucccuaaug ugccucgcac uaggcggagu ccuaauauuc     2040

cuaucaaccg ccgucucagc a                                               2061


<210>  2940
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short CDS opt

<400>  2940
accuccgugg gaaucguagg acugcuacuc acaacagcca uggcagcaga agucacccga       60

agaggcucug cguacuacau guaccuagac cgcaacgacg ccggggaagc gauaucauuc      120

ccaacaacac ucggcaugaa caaaugcuac auccaaauaa uggaccuagg ccacaugugc      180

gacgccacaa ugagcuacga augcccaaug cucgacgaag gaguggagcc agaugaugug      240

gacugcuggu gcaacacgac cucaaccugg guaguauacg gcacaugcca ccacaaaaag      300

ggcgaggccc gcagaagccg cagagcaguc acccuacccu cacacucuac aagaaaacua      360

caaacacgcu cccaaacaug gcuagaaagc agagaauaca caaaacaccu aauacgagua      420

gagaacugga ucuuccgaaa cccaggcuuc gcauuggcag ccgccgcaau cgcuuggcua      480

cuagggucca gcaccagcca aaaagucauc uaccuaguua ugauacuccu caucgcccca      540

gccuacagca uccgaugcau ugguguauca aacagggacu ucguagaagg aaugagcggc      600

ggaacauggg uagacguggu ccucgaacac ggcggcugcg uaacgguaau ggcccaagac      660

aaacccacag uagauauaga gcuagucaca acgacaguaa gcaacauggc cgaaguaaga      720

agcuacugcu acgaagcaag cauaagcgac auggccucgg acuccaggug ccccacgcag      780

ggcgaagcau accuagacaa gcagagugac acccaauacg ucugcaagcg cacgcuagua      840

gacagaggau ggggaaacgg uugcggccua uucggaaaag ggucacucgu gaccugcgca      900

aaguucgccu gcaguaaaaa gaugacaggc aaaagcauac aacccgagaa ccuggaauac      960

cguaucaugc ucagcguaca cggcucacaa cauagcggga ugauagucaa cgacacaggc     1020

cacgaaacgg acgagaacag agcaaaaguc gaaauaaccc caaacucacc ccgagcagaa     1080

gcaacccuag gcggcuucgg aucccuggga cuagacugcg aacccagaac aggccuugac     1140

uuuuccgauc uguauuaccu gacaaugaac aacaagcacu ggcuaguaca caaagaaugg     1200

uuccacgaca uaccccuacc cuggcacgca ggcgccgaca ccggcacacc ccacuggaac     1260

aacaaagaag cccucguaga guucaaagaa gcccacgcaa agcgccaaac gguagucgua     1320

cuggggagcc aagagggagc cguacacaca gcgcuagcgg gagcacucga agcagaaaug     1380

gacggggcca aagggagacu uagcagcggc caccuaaaau gccgacuaaa aauggacaaa     1440

cuccgccuaa agggaguauc auacucccug ugcacagcag cauucaccuu caccaagauc     1500

cccgcagaaa cacuccaugg cacagucaca guagaaguac aguacgccgg caccgacgga     1560

cccugcaaag uaccagcaca aauggccgua gacaugcaaa cacuaacccc aguaggcaga     1620

cucaucacag cuaauccagu aauaacagaa agcaccgaga acaguaaaau gaugcuggag     1680

cucgacccgc ccuucggcga cuccuacauc guaauaggag ugggcgagaa aaagauaaca     1740

caccauuggc acagaagcgg cagcaccaua ggcaaagcau ucgaagcgac ggugcgcggg     1800

gcuaaacgca uggcagugcu aggcgacacc gcgugggacu ucggcucagu aggaggggca     1860

cuaaacucac ucggaaaagg cauacaccag aucuucggag cagccuuuaa gagccucuuc     1920

ggaggcauga gcugguucuc gcaaauacuc aucggcacac uucucaugug gcucgggcua     1980

aacaccaaaa acggaucaau cagccucaug ugccuagcac uaggaggggu ccucaucuuc     2040

cuaagcacgg cggucagugc c                                               2061


<210>  2941
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short CDS opt

<400>  2941
accagcguug gaauagucgg ccuacuacua accacagcaa uggccgccga agucacacgc       60

agaggguccg cuuacuacau guaccuagac cgaaacgacg cgggcgaagc aaucagcuuc      120

cccaccaccc ucggaaugaa caaaugcuac auccaaauca uggaccuagg acacaugugc      180

gacgcgacua ugaguuacga augccccaug cucgacgaag gagucgaacc ggacgacgua      240

gacugcuggu gcaacaccac guccaccugg guaguauacg gaaccugcca ccacaagaaa      300

ggcgaagcac gccgaagccg ccgcgccguc acgcugccau cucacucaac acgaaaacua      360

caaacacgaa gucagacaug gcuagagagc cgcgaguaca ccaaacaccu gauaagaguu      420

gaaaauugga uauucagaaa cccaggcuuc gcgcuggcgg cagcagccau cgccuggcuc      480

cucggaucau ccaccagcca gaaagucaua uaccuaguaa ugauccuccu caucgcacca      540

gcguacagca uccgcugcau cggcguaagc aaccgcgauu ucguagaagg caugagcgga      600

ggcaccuggg uagacguagu acucgaacac ggaggaugcg uaaccgucau ggcccaagac      660

aaacccacag ucgacaucga acucgucacc accacaguaa gcaacauggc cgagguccgc      720

agcuacugcu acgaggcaag caucagcgac auggcaucgg acucccgaug cccaacccaa      780

ggggaagcgu accuagauaa acaaagcgac acacaauacg ucugcaaacg cacacuugua      840

gaccgaggau gggguaacgg augcggacuc uuuggcaaag gcagccucgu cacaugcgcc      900

aaauucgcau gcucaaagaa aaugacagga aaauccaucc agcccgagaa ccuggaguac      960

cgcaucaugc ucagugucca cgggagccaa cacucaggca ugaucgucaa cgacacgggc     1020

cacgaaaccg acgaaaaccg agccaaaguc gaaaucaccc ccaacucgcc aagagcagaa     1080

gccgcccucg gcggcuucgg aucacucgga cuggacugcg aacccagaac uggacucgac     1140

uucucagacc ucuacuaccu caccaugaac aacaaacacu ggcucgucca caaagaaugg     1200

uuccacgaca uaccccuacc cuggcacgcc ggugcugaca caggcacucc acacuggaac     1260

aacaaagaag cccuaguaga guucaaagac gcacacgcaa aacgccaaac cguagucgua     1320

cuagggagcc aagaaggcgc cguacacacc gcacuagcag gcgcacuaga agcagaaaug     1380

gacggcgcaa aaggcagacu gucuagcggc cacuuaaaau gccgucucaa aauggacaaa     1440

cugagacuca aaggaguaag cuacucccua ugcacagcag cauucacauu cacaaaaaua     1500

cccgcagaaa cacuacacgg cacaguaacc gucgagguac aauacgcagg gacagacggg     1560

cccugcaaag ucccagcaca aauggccgug gauaugcaaa cccucacacc cguaggacga     1620

cuaauaaccg caaacccagu cauuacagaa uccaccgaaa acagcaaaau gaugcucgaa     1680

cuagacccac cauucggcga cucauacauc gucauaggag ucggggaaaa gaaaaucaca     1740

caccacuggc accgcagcgg gucgaccauc ggcaaagccu ucgaagcgac aguuagaggg     1800

gcaaaaagga uggccguccu aggcgacacc gcgugggacu ucggcuccgu aggcggagcc     1860

cuaaacaguc ucggcaaagg aauacaccaa aucuucggag cagcguucaa aucacuauuu     1920

ggcggcaugu cuugguucuc acaaacccua aucgggaccc uccucaugug gcucggccua     1980

aacaccaaaa acggcuccau cagccucaug ugcuuagccc ucggcggggu ccuaauauuc     2040

cuuuccacag cagucagcgc c                                               2061


<210>  2942
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short CDS opt

<400>  2942
accagcguag gaaucgucgg ccuccuacuu accaccgcaa uggccgcaga agucacccgu       60

cgaggcucag ccuacuacau guaccucgac agaaacgacg ccggcgaagc caucagcuuu      120

ccgacaacgc uuggcaugaa caaaugcuac auacaaauaa uggaccucgg ccacaugugc      180

gacgcaacga ugucauauga augcccaaug cuagacgaag gcguagaacc agacgacguc      240

gacugcuggu guaacacaac auccaccugg guggucuacg gaaccugcca ccacaagaaa      300

ggagaagcuc gacgaagccg acgcgccgua acacugccca gccauuccac gcgcaaacuc      360

caaacacgcu cccaaaccug gcuugagagc cgagaguaca caaaacaccu aauacgcgug      420

gaaaacugga uauuccgaaa ccccggcuuc gcacucgccg cagccgcaau agcguggcuu      480

cuaggaagcu ccaccucaca gaaagugaua uaucugguaa ugauacugcu cauagcaccc      540

gccuacagca uaagaugcau cggcgucuca aacagggacu ucgucgaagg aaugagcgga      600

gggacguggg ucgacgucgu ccuagaacac gggggcugcg uaacagucau ggcgcaagac      660

aagcccacgg uagacaucga acuggucacc accacagucu caaacauggc cgaaguacgc      720

uccuauugcu augaagccuc aaucucagac auggcguccg acagccgcug ccccacccaa      780

ggcgaagccu accucgacaa acaaucggac acccaauacg ucugcaaaag aacacuugua      840

gacaggggau ggggcaacgg augcggacuc uucggcaagg gcagccuagu aacaugcgcc      900

aaauucgccu gcucaaagaa aaugaccgga aaguccaucc aacccgaaaa ccuagaauac      960

agaaucaugc uauccguaca cggcagccaa cacuccggca ugaucgucaa cgacaccggc     1020

cacgaaacag acgaaaaccg agccaagguc gaaauaaccc ccaacucacc cagggcggaa     1080

gcaacacuug gcggauucgg cagccucggc cuagacugcg aaccacgcac cggccuagac     1140

uucagcgacc ucuacuaccu aacaaugaac aacaaacacu ggcucguaca uaaggagugg     1200

uuccaugaca uaccgcuacc cuggcacgca ggcgccgaca ccggaacccc acacuggaac     1260

aacaaagaag cgcucgucga guucaaagac gcccacgcca aacgccagac cguagucguc     1320

cuaggaucac aagaaggcgc uguacacaca gcccucgccg gagcccucga agccgaaaug     1380

gacggagcaa aaggccgccu cagcagcggg caccuaaaau gccgacuaaa aauggacaaa     1440

cuccgucuca aaggaguaag cuacucccuc ugcacagccg cuuucaccuu cacaaaaaua     1500

ccagccgaaa cccuccacgg caccgucaca guagaagucc aauacgcagg ggcugacgga     1560

cccugcaaag uccccgccca aauggccgua gacaugcaaa cgcucacccc agucggcaga     1620

cuuauaaccg cgaaccccgu aaucaccgaa agcaccgaaa acucaaaaau gaugcucgag     1680

cuagacccac ccuucggcga cagcuacauc gucaucggcg ugggagaaaa gaagaucaca     1740

caccacuggc acagaagcgg cucgacaaua ggcaaagcau ucgaagccac cguacguggc     1800

gcgaaacgca uggccgugcu cggagacacc gcuugggacu ucgggagcgu agggggagcc     1860

cucaacuccc ucggcaaggg aauacaccaa auauucggcg cugccuucaa gagccucuuc     1920

ggcggaauga guugguucuc ccaaauacuc aucggaacuc uccuaaugug gcucggacuc     1980

aacaccaaaa acggcucgau uucccucaug ugccuugcac uuggcggugu ccuuauauuu     2040

cucuccaccg ccguaucagc a                                               2061


<210>  2943
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short CDS opt

<400>  2943
accagcgucg gcauuguagg ccuauuacuu acaacagcua uggccgccga agucacaaga       60

cggggauccg ccuacuacau guaccuagac cgcaacgacg ccggagaagc cauaucauuc      120

cccacaaccc uaggcaugaa caagugcuac auacaaauaa uggaccuugg ucacaugugc      180

gacgccacaa ugagcuacga augucccaug cucgacgaag gcguagagcc cgacgacguc      240

gacugcuggu gcaacacaac auccacgugg guaguauacg ggaccugcca ccacaagaaa      300

ggcgaggcca gacggucacg acgagccgua acccuacccu cccacuccac ccgaaaacuc      360

caaacacgca gccaaacuug gcuagaauca cgagaauaca ccaaacaccu aauccgcgua      420

gagaacugga uauucagaaa cccaggauuc gcacuagccg ccgccgcaau agccuggcuc      480

cuaggcucau cgaccucaca gaaaguaauc uaccucguaa ugauacuucu caucgcacuc      540

gccuacagca uccgcugcau cggcguaagc aaucgcgacu ucgucgaggg aaugucagga      600

ggaacauggg ucgacgucgu gcuagagcac ggcgggugcg uaacggucau ggcacaagac      660

aaacccaccg ucgacaucga acucguaacg accacagucu cuaacauggc cgaaguacga      720

aguuacugcu acgaagccuc cauaucggac auggcaagcg acucccgcug cccgacccaa      780

ggagaagccu accuggacaa acagagcgac acacaauacg ugugcaaacg aacucuaguc      840

gaccgcggau ggggcaacgg augcggccuc uucggaaaag gaagccuagu aacaugcgcg      900

aaauucgcuu gcuccaagaa aaugaccggg aaauccaucc aaccugagaa ccuggaauau      960

cgaauaaugc uauccguaca cggcucccaa cacuccggca ugaucgucaa cgacacugga     1020

caugaaacag acgaaaaccg cgccaaagug gaaaucacac ccaacucccc acgcgcggaa     1080

gcaacgcucg gaggauucgg cucacuaggc cuagacugcg aacccagaac cggacuagac     1140

uucagcgacc ucuacuaccu cacaaugaac aacaaacacu ggcuagucca caaagaaugg     1200

uuccacgaca ucccccuacc auggcacgca ggggcggaca cgggaacacc ccacuggaac     1260

aacaaagaag cucucguaga guucaaagac gcgcaugcga aacgacaaac cguaguaguc     1320

cucggaucgc aagaaggagc aguacacacc gcccuggccg gcgcccuaga agccgaaaug     1380

gacggagcaa aaggccgacu cagcagcggg caccucaaau gcagacuaaa aauggacaaa     1440

cuaagacuca aaggcguaag cuacagccuu ugcacagcag ccuuuacauu cacaaaaauu     1500

cccgcugaaa cacuccacgg caccguaacc guagaagucc aauacgcagg caccgacgga     1560

ccaugcaaag ucccagcaca gauggcuguc gacaugcaaa cacucacacc cguaggacga     1620

cuaauaacug ccaaccccgu gaucaccgaa agcacagaaa acagcaagau gaugcucgaa     1680

cuggacccac ccuucggcga cuccuauauc gucauuggag ucggagaaaa gaaaaucacc     1740

caccacuggc accgcagcgg cuccacaauc ggcaaggccu ucgaagcaac cguaagaggc     1800

gccaaaagaa uggccguacu aggagacacg gcaugggacu ucggcagcgu aggcggggca     1860

cucaacaguc uaggaaaggg cauccaccaa auauucggcg cagccuucaa aucacuauuc     1920

ggcggcauga gcugguucag ucaaauacuc auaggcacgc ugcuaaugug gcucggccuc     1980

aauaccaaaa acggaucgau cucccucaug ugccucgccc uuggaggcgu gcuaaucuuc     2040

cuauccaccg cagucagcgc a                                               2061


<210>  2944
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short CDS opt

<400>  2944
accuccguag gaauagucgg ccuccuccug accacagcca uggccgccga agugaccaga       60

cgcggcucag cauacuacau guaccucgac aggaacgacg ccggcgaagc caucucauuc      120

ccgaccacau uaggcaugaa caaaugcuac auccaaauca uggaucuagg ccacaugugc      180

gaugccacca ugucauacga augccccaug cucgaugaag gcgucgaacc ggacgacgua      240

gacugcuggu gcaacaccac cuccaccugg gucguauacg gaaccugcca ccacaagaaa      300

ggugaagcac gcagaagccg acgcgccgug acacuucccu cacacuccac ccgaaaacua      360

caaacacgcu cacaaaccug gcuagaaucc cgcgaauaca ccaaacaccu cauacgcguc      420

gaaaacugga ucuuucgaaa cccaggauuc gcucucgccg cagccgcaau cgcauggcuu      480

cucggaucuu ccacauccca aaaagucauc uacuuaguua ugauccuccu caucgcccca      540

gccuacucca uacgaugcau cggcguaagc aaccgcgacu ucgucgaagg caugucggga      600

ggcacguggg uagacgucgu ucuagaacac gguggcugcg uaacaguaau ggcccaagac      660

aagcccaccg ucgacauuga acucgucacc accacuguau caaacauggc ugaaguccgc      720

agcuacugcu acgaagcaag caucuccgac auggucuccg acagccgcug cccaacccaa      780

ggcgaagcgu accuagacaa acagucggac acccaauacg ucugcaaacg cacccuaguc      840

gacagagguu ggggcaacgg augcggacuc uucggcaagg gaucccuagu aacaugugcc      900

aaauucgcgu gcucgaagaa aaugaccgga aaaagcaucc agcccgaaaa ccucgaauac      960

cgcauaaugc ucuccgucca cggaagccaa cacuccggca ugaucgugaa cgacaccgga     1020

cacgaaaccg acgaaaaccg cgcuaaagua gaaaucacac caaacucacc ccgcgcagaa     1080

gcaacccuag gaggcuucgg aucccucggc cuagacugug aaccccggac cggccuagau     1140

uucuccgacc ucuacuaccu aaccaugaac aacaaacacu ggcucguaca uaaagagugg     1200

uuucacgaca ucccccuccc cuggcacgcg ggcgcagaca cagguacacc ccauuggaac     1260

aacaaagaag cccuugucga guucaaagac gcacacgcca aaagacaaac cgucgucgug     1320

cuuggcuccc aggaaggagc cgugcacaca gcccuggccg gagcgcuaga agcagaaaug     1380

gacggcgcca aaggaagacu aagcuccgga caccuaaaau gccgacuaaa aauggacaaa     1440

cuucgacuua aaggaguguc auacucacuc ugcacggccg cauuuaccuu caccaaaauc     1500

cccgcagaaa cacuacacgg caccgucacc gucgaaguac aauacgccgg aaccgacggc     1560

ccaugcaagg uaccagcaca aauggccguc gacaugcaga cacuuacccc gguaggccga     1620

cugauaaccg cuaacccagu caucaccgaa ucaaccgaaa auucgaaaau gaugcuggaa     1680

cucgacccac ccuucggaga cucauauauc gucauaggcg uaggagaaaa gaaaaucacc     1740

caccacuggc accgcucugg cagcacaaua ggaaaagccu ucgaagccac agucagaggc     1800

gccaaacgca uggccgugcu cggcgacacc gccugggacu ucggaagcgu cggaggcgcc     1860

cucaacagcc ugggcaaagg aauccaccaa auauucggag cagccuuuaa aucccuuuuc     1920

ggaggaaugu caugguucuc ccaaauucua auuggcacac uccuaaugug gcucggacua     1980

aacaccaaaa auggcucaau aagccugaug ugucuagcac uaggaggcgu acucaucuuc     2040

cuuagcaccg ccgucagcgc a                                               2061


<210>  2945
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short CDS opt

<400>  2945
accuccguag gcauagucgg ccuacuccug acaaccgcaa uggcagccga agucacacgc       60

cgcggaagcg ccuacuacau guaccucgac cgaaaugacg ccggcgaagc caucagcuuc      120

cccaccacgc ucggcaugaa caaaugcuac auacaaauca uggaccucgg ccacaugugc      180

gacgccacca ugucguacga augccccaug cuagacgaag gcgucgaacc agacgacgua      240

gacugcuggu gcaacaccac aucgacaugg guaguguacg gaacaugcca ccauaagaaa      300

ggagaagcaa gacgcagcag gcgagcgguc acccuaccca gccacuccac gagaaaacuc      360

cagacacgcu cccaaacaug gcucgaaagc agagaauaca cuaaacaccu cauccgagua      420

gaaaauugga uauuuagaaa cccaggcuuc gcgcuagcgg cagccgcaau cgccuggcua      480

cucgguaguu ccacaucaca aaaaguaauc uaccucguaa ugauccuccu aauugcacca      540

gcauacucca uaagaugcau cggcaucucc aacagagacu ucguggaagg caugucaggg      600

ggaacauggg uagacguugu acucgagcac ggcggcugcg uaaccguaau ggcccaagac      660

aaaccaacag uggacaucga acucguuacc acaaccguca guaacauggc cgaaguccgu      720

uccuacugcu acgaggccag uauauccgac auggccagcg acagccgcug cccaacccaa      780

ggggaagccu accucgacaa acaaucagac acccaauaug uuugcaaaag aacccuaguc      840

gaccgaggcu ggggaaacgg augcggacuu uucggcaaag gcucgcucgu cacaugcgcc      900

aaauucgcau gcuccaagaa aaugaccggc aaaagcauac aaccagaaaa ccucgaauac      960

cgcaucaugc ucuccguaca cgguagccaa cacagcggga ugaucgucaa cgacacaggc     1020

cacgaaaccg acgaaaaccg cgcgaaaguc gaaauaaccc cuaacucacc ccgugccgaa     1080

gcaacacuug gcggguucgg uauccugggc cucgacugcg aacccagaac cggccucgac     1140

uucuccgacc ucuacuaccu uaccaugaau aacaaacacu ggcuagucca caaagaaugg     1200

uuccacgaca uaccgcuccc cuggcacgcc ggagccgaca caggcacacc ccacuggaac     1260

aacaaagaag cccugguaga guucaaggac gcgcacgcaa aacgacagac cguagucgug     1320

cucggcaguc aagaaggugc ugugcacacg gcacucgccg gugcacugga agcagaaaug     1380

gacggagcaa aaggccgccu auccagcggc caucucaaau gccgacucaa aauggacaaa     1440

cuacgucuaa aaggaguguc guacagccua ugcacagccg ccuucacauu cacgaaaauc     1500

ccagccgaaa cacuacacgg aacgguaaca guagaaguac aauaugcagg caccgacgga     1560

ccaugcaaag uacccgccca aauggccguu gacaugcaaa cucuaacucc aguaggacgc     1620

cugauuaccg ccaacccagu caucaccgaa agcaccgaaa acucuaaaau gaugcucgaa     1680

cuggacccac cauucgguga cagcuacauc gucauaggcg uaggagaaaa gaaaaucacc     1740

caccacuggc accgcagcgg cuccaccaua ggcaaagcau ucgaagcaac cguccgggga     1800

gccaaacgca uggcaguccu cggcgacacc gccugggauu ucggguccgu cggcggggcc     1860

cucaauuccc uggggaaagg aauacaccaa aucuucgggg ccgccuucaa aagccuuuuc     1920

ggcggaaugu caugguucuc ucaaauacuc aucggaaccc uacuaaugug gcuuggccug     1980

aacaccaaaa acggaagcau cucacucaug ugccucgcac uuggaggcgu gcucaucuuc     2040

cuauccaccg cagucagugc c                                               2061


<210>  2946
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short CDS opt

<400>  2946
acaagcguag gcaucguagg acuccuccua acaaccgcca uggcagcaga aguuacacgc       60

cggggaucag cauacuacau guaccuagac cgcaacgacg ccggugaagc caucucguuc      120

cccacaacac ugggcaugaa caaaugcuac auccaaauca uggaccucgg acauaccugc      180

gacgcaacaa ugucauauga augccccaug cuagacgaag gcgucgaacc cgacgacgua      240

gacugcuggu gcaacaccac cagcacgugg guagucuaug gcaccuguca ccacaagaaa      300

ggcgaagcac gccguucucg cagagcaguc acccuacccu cacacagcau ccgcaaacuu      360

caaacccgcu cccaaacaug gcucgagucc cgggaauaua cgaaacaccu cauacgagua      420

gaaaacugga ucuuccgcaa ccccggcuuu gcccuagccg cagcagcaau agccuggcua      480

uuaggcucca gcaccagcca aaaaguaauc uaccugguca ugauccuccu cauugccccu      540

gcauacucaa uacgcugcau cggagucuca aacagggauu ucgucgaggg aaugucgggc      600

ggaacguggg ucgacgucgu ccucgaacac gguggcugcg ugacagucau ggcccaagac      660

aaaccgacag uugacauaga acuugucacc acaacaguau caaacauggc ggaaguccgc      720

agcuacugcu acgaagccuc aauauccgac auggcaagcg acagccgaug cccaacccaa      780

ggcgaagcau accucgacaa acaauccgac acgcaauaug uaugcaaacg cacccuagua      840

gaccggggcu ggggaaacgg cugcggacuc uucggcaaag gcagccuagu cacaugcgcu      900

aaauucgccu guagcaagaa aaugacaggc aaaagcaucc aacccgaaaa ucucgaguac      960

cgaaucaugc uaagcguaca cggcucacaa cacucaggaa ugauagucaa cgacacagga     1020

cacgagacag acgaaaaccg cgcaaaaguc gagauaacgc ccaacucccc acgcgcagaa     1080

gcaacacucg gcggcuucgg aagucucggu cuagacugcg aaccccgaac aggacuagac     1140

uucucagacc uauacuaccu gacuaugaac aacaagcacu ggcucgucca caaagaaugg     1200

uuucacgaca uccccuuacc auggcacgca ggcgcugaca ccggcacacc acacuggaac     1260

aacaaagaag cacucgucga guucaaagac gcucacgcca aacggcaaac cgucguuguc     1320

cugggcuccc aagaaggcgc gguacacacc gcccucgccg gcgcucucga ggcagaaaug     1380

gacggagcaa aaggacgccu cuccagugga caccucaaau gcagacuaaa aauggacaaa     1440

cuacgccuca aaggugucuc auacagccuc uguacagcag cauucaccuu caccaaaauc     1500

ccggccgaga cacuccacgg aaccguaacc guagaagucc aauacgcagg gacagacgga     1560

cccugcaaag ucccagcaca aauggcagua gacaugcaaa cacuaacacc cgugggaaga     1620

cucaucaccg caaaccccgu caucaccgaa agcaccgaaa acagcaaaau gaugcuagaa     1680

cuagacccac ccuucggcga cucauacaua guaauaggcg ugggagaaaa gaaaaucacc     1740

caccacuggc accgcagcgg aucaaccaua ggcaaagcau ucgaagccac aguccgcggu     1800

gcaaaacgca uggccguccu cggcgacaca gccugggacu ucggcagcgu aggcggcgcc     1860

cuaaacucgc uaggaaaagg cauacaccag aucuucgggg cagccuucaa gagucucuuc     1920

ggcgguaugu ccugguucag ccaaauccuc aucgggaccc uacucaugug gcucggccuc     1980

aacgccaaaa acggcucgau cucacucaug ugccuagcac ucggcggcgu ccucauauuc     2040

cugucaacag ccguaagcgc c                                               2061


<210>  2947
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short CDS opt

<400>  2947
acaucagucg ggauagucgg ccuacuacua accacggcca uggcagccga aguaacccga       60

cgcggaucgg cauacuacau guaccuagac agaaacgacg caggagaagc cauaucuuuc      120

cccaccacau uaggcaugaa caaaugcuac auccaaauca uggaccuagg acacaugugc      180

gacgcaacga ugucguauga gugccccaug cucgacgaag ggguggaacc cgacgacgua      240

gacugcuggu gcaacacaac cucaaccugg gucguauacg gaaccugcca ucauaagaaa      300

ggggaagccc aacgcucccg cagagccgua acacuaccca gccacagcac cagaaaacua      360

caaacccgcu cacaaaccug gcuggaauca cgcgaguaca ccaaacaccu aauaagggua      420

gaaaacugga uauuccggaa cccaggcuuc gcccucgccg cagcagcaau agcauggcua      480

cucggauccu caacauccca gaaagugauc uaccucguua ugauacuccu uaucgccccc      540

gccuacagca uccgaugcau aggcgucagc aaccgagacu ucguagaagg aaugagcggc      600

ggcaccuggg uagacgucgu acucgagcac ggcgguugug ucaccgucau ggcccaggac      660

aaaccaacag uagacauuga acucgugaca acaacggucu ccaacauggc agaaguacgc      720

agcuacugcu acgaagccag caucagcgac auggccuccg acagccgcug ccccacacaa      780

ggagaagcgu accuggacaa acaaucagac acccaauacg ucugcaaacg cacccuaguc      840

gaccgaggau ggggcaacgg augcggccuc uucggcaaag gaucgcucgu aacaugcgcc      900

aaauucgccu gcagcaagaa aaugaccgga aaaucaaucc aacccgaaaa cuuagaauac      960

cgcauaaugc uaucagucca cggcucacaa cacagcggca ugaucguaaa cgacaccggc     1020

cacgaaaccg augagaaccg cgcaaagguc gaaauaaccc ccaacagccc ccgcgccgaa     1080

gcaacccuag gaggauuugg aagccucggc cuagacugcg aaccacgcac cggccucgac     1140

uucuccgacc uauacuaccu caccaugaac aacaagcacu ggcuggucca caaagaaugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggagccgaca cgggaacacc ccacuggaau     1260

aacaaagagg cccuaguaga guucaaagac gcacacgcga aacggcaaac aguaguugua     1320

cucggaucac aagaaggggc cguacacaca gcccuagcag gagcacucga agcggaaaug     1380

gacggagcca aaggcagacu cagcagcggg caucuaaaau gccgacucaa aauggacaaa     1440

cuccgauuga aaggcgucag cuacagccuc uguaccgcag cauuuacauu caccaagaua     1500

cccgccgaaa cacuacacgg aacaguaacc guagaagucc aauacgccgg gacagacggg     1560

cccugcaagg uacccgcaca gauggcagug gacaugcaaa ccuuaacacc gguaggacga     1620

cugaucacag cgaacccagu caucacggaa ucaaccgaaa acuccaaaau gaugcuggaa     1680

cuagacccgc cuuucggcga uagcuacauc guaauuggag ugggcgaaaa gaaaaucaca     1740

caccacuggc accgcuccgg cucaacaauc ggaaaagccu ucgaagccac cguccgcggc     1800

gccaaaagaa uggcaguccu aggagacaca gcaugggacu ucgguagcgu aggcggagcc     1860

uuaaacucac ugggaaaagg cauccaccaa aucuucggag ccgcauucaa aucacuauuc     1920

ggaggaaugu ccugguucag ucaaauacuc aucggcacgc uacucaugug gcuaggacuc     1980

aacaccaaaa acggaagcau cucgcugaug ugccuagccc uaggaggcgu acucauauuc     2040

cuauccacag cggucucagc a                                               2061


<210>  2948
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short CDS opt

<400>  2948
acaagcgucg gaauaguggg acuccuacuc acaacagcaa uggcggccga ggucacacga       60

cgaggaagcg ccuacuacau guaccuagac cgcaacgacg caggagaggc caucucguuc      120

cccacgaccc uaggcaugaa caaaugcuac auccagauca uggaccucgg ucacaugugc      180

gacgcaacaa ugucguacga augccccaug cucgacgaag gggucgaacc agacgacgua      240

gacugcuggu gcaacacaac aagcaccugg gugguauacg gcaccuguca ccacaagaaa      300

ggagaagccc gaagaagccg cagagccgua acacucccaa gccacuccac ccggaagcua      360

caggcacgca gccaaaccug gcuagaaagc agagaauaca ccaaacaccu cauacgcguc      420

gaaaacugga uauucagaaa ccccggauuc gcccuagccg ccgccgccau cgcguggcuu      480

uuagguuccu ccacuagcca aaaaguaaua uaccuaguaa ugauacuccu aauagcgcca      540

gccuacagca ucagaugcau aggcguaucc aacagagacu ucgucgaagg cauguccgga      600

ggaacauggg ucgaugucgu acuagaacac ggcggcugcg uaaccgucau ggcccaagac      660

aaaccaacag uagauauaga acucgucacu accacgguaa gcaacauggc cgaggugcgc      720

ucauacugcu acgaggccag caucucagac auggcaucag acucacgcug cccaacacaa      780

ggagaagcau acuuagacaa acaaagcgac acacaauacg uaugcaaacg cacccuagua      840

gauagaggcu ggggcaacgg gugcggacuc uucggcaaag gaucccuagu cacaugcgcg      900

aaauucgccu gcucaaagaa aaugacagga aaaucaauac aaccagaaaa ccucgaauac      960

cgcaucaugc uaagcgugca cggaucacaa cacuccggca ugaucguaaa cgacacaggc     1020

cacgaaacag acgaaaaccg agcaaaagug gaaauaacac cuaacucacc acgcgccgaa     1080

gccacccuag ggggcuucgg cucacucggc cucgacugcg aacccaggac aggccuggac     1140

uuuucggacc uguacuaccu uacaaugaac aacaaacacu ggcuaguaca uaaagaaugg     1200

uuccacgaca uuccacuucc cuggcacgca ggagccgaca ccggcacccc acacuggaac     1260

aauaaggaag cacucguaga guucaaagac gcccacgcca agagacagac aguagucgua     1320

cucggauccc aggagggcgc aguacacacg gcccuagcgg gcgcacuaga agccgagaug     1380

gacggcgcca aaggccggcu cagcagcggc caccucaaau gccggcuuaa aauggacaag     1440

cucagacuga aaggcguauc auacagccua ugcaccgccg ccuucacguu cacaaaaauc     1500

ccagcggaaa cacuacacgg aaccgucaca gucgaagugc aauacgcggg cacagacggc     1560

cccugcaaag ugcccgccca aauggcgguc gacaugcaaa cacuaacccc aguaggaaga     1620

cucaucaccg caaacccggu cauaacagaa agcacagaaa acagcaagau gaugcuagaa     1680

cuagacccac cauucggcga cucauacaua gucaucggcg uaggagaaaa gaaaaucaca     1740

caccacuggc accgaagcgg cucuacaauc ggaaaagccu ucgaagccac cguacgcggc     1800

gcgaaacgca uggccguccu cggagacaca gcaugggacu uuggcagcgu aggaggcgcc     1860

cucaacuccc uaggcaaagg cauccaccaa auauucgggg ccgccuucaa aucccucuuc     1920

ggaggaaugu caugguucag ccagaucuua auaggaaccc ugcuaaugug gcuaggccuc     1980

aacacgaaaa acggguccau aucgcuaaug ugccuagcac ugggcggagu ccucauauuc     2040

cuaucaacag ccguauccgc c                                               2061


<210>  2949
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short CDS opt

<400>  2949
acaagcgucg gcaucguagg ccuccuccug accacagcca uggcagccga aguuacacgc       60

cgaggaucag cauacuacau guaccucgac cgcuccgacg cgggagaagc caucagcuuc      120

ccuaccaccc ucggcaugaa caaaugcuac auacaaauaa uggaccuggg acacaugugc      180

gacgcaacaa ugucguacga augcccaaug cuagacgaag gcgucgaacc cgaugacgua      240

gacugcuggu gcaacaccac auccaccugg guggucuacg gaaccugcca ccacaaaaag      300

ggcgaggcca gacggucgag acgcgcgguc acccuuccua gccacagcac cagaaaacua      360

caaacacggu cgcagacuug gcucgaaagu agagaguaua ccaaacaccu cauccggguc      420

gaaaauugga ucuuccgcaa ccccggauuc gcgcucgccg ccgccgcaau agcguggcua      480

cucggcagcu ccacaagcca aaaagucaua uaccucguua ugauacuccu caucgccccc      540

gccuacucca uucgaugcau aggcguaagc aacagagacu ucguagaagg caugagugga      600

gggaccuggg ucgacguagu ccucgaacac ggcggcugcg ucaccguaau ggcgcaagac      660

aaacccacag uagacaucga gcuagucacc accaccguaa guaacauggc agaaguccga      720

uccuacugcu acgaagcauc aaucuccgac auggccuccg auagccggug ccccacacaa      780

ggcgaagcau accucgacaa gcaaagcgac acucaguacg ucugcaaaag aacacucguc      840

gaccgaggcu ggggaaaugg augcggccua uucgguaaag gcucccucgu aaccugcgcc      900

aaauucgcau guucgaagaa aaugaccgga aaaagcauac aacccgagaa ccuagaauac      960

cguauaaugc uaagcgucca cggaagccaa cacagcggaa ugaucgucaa cgacacaggc     1020

caugaaacag acgaaaaccg cgccaaagua gaaauaaccc cuaacucacc aagggcagaa     1080

gcaacccuag gcggauucgg aagccuagga cucgacugug aaccaaggac aggacucgac     1140

uucucagacc uauacuaccu caccaugaac aacaaacacu ggcuggucca caaagaaugg     1200

uuccacgaca uuccgcuacc guggcaugcu ggcgccgaua caggcacgcc ccauuggaac     1260

aacaaagaag cacucguaga guucaaagac gcccacgcaa aacgccaaac cgucgugguc     1320

cuagggagcc aggaaggagc cguccacacc gcccucgccg gagcccucga agccgaaaug     1380

gacggcgcaa aaggccgcuu aagcuccggc caccuaaaau gccgccugaa aauggacaaa     1440

cucaggcuca aaggcguaag cuacucgcua uguaccgcag cauucacauu cagcaaaauc     1500

ccagcagaaa cacuacacgg cacagucaca gaagaaguac aguacgccgg caccgacggc     1560

cccugcaaag uccccgcgca aauggccguc gauaugcaaa cacuaacacc cguaggccga     1620

cuaauaacag caaaccccgu aaucaccgaa agcaccgaaa acagcaaaau gaugcuggaa     1680

cucgacccgc ccuucggaga cucauacauc guaauaggcg ucggagaaaa gaaaaucacu     1740

caccacuggc accgcuccgg cagcaccauc ggcaaagccu ucgaagccac cguccgcggc     1800

gccaagcgaa uggcgguacu aggcgacaca gccugggacu ucggaucagu uggaggagcc     1860

cuaaacucac uaggcaaggg cauacaccaa aucuucggag cagccuucaa aucccuauuc     1920

gggggcauga gcugguucag ccaaauccua aucggaaccc uccucaugug gcucggccua     1980

aacaccaaaa acggcuccau uagucuaaug ugccuagccc ucggcggcgu gcucaucuuc     2040

cuauccacag cgguaucagc c                                               2061


<210>  2950
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short CDS opt

<400>  2950
accucagucg gaauaguagg acuacuccuc acgacagcaa uggccgcaga aguaaccaga       60

cgcgguuccg ccuacuacau guaccucgac cgcuccgacg ccggagaagc caucagcuuc      120

cccacaaccc uaggcaugaa caaaugcuac auacaaauca uggaucucgg acacaugugc      180

gacgccacca uguccuacga augccccaug cucgacgaag gaguagagcc ggacgacguu      240

gacugcuggu gcaacacaac auccacgugg guagucuacg gaaccugcca ucacaagaaa      300

ggcgaagccc gaagaucccg ccgcgccguc acacuaccca gccacucaac gcggaaacuc      360

caaacaagau cgcaaaccug gcuagaaagc agagaauaua ccaaacaccu aaucagagua      420

gagaacugga uauucaggaa cccaggcuuc gcacuagccg ccgcagccau agccuggcuc      480

cucgguuccu ccacaagcca aaaaguaaua uaccucguca ugauacuccu caucgcacca      540

gcauauucga uacguugcau aggagugagc aauagagacu ucguagaggg aaugucagga      600

ggcaccuggg uagacgucgu acucgagcac ggcggaugug uaacaguuau ggcccaagac      660

aaacccaccg ucgacauaga acucgucaca accacagucu caaacauggc cgaaguaaga      720

uccuacugcu acgaggcuuc caucucagac auggcauccg acucacgcug cccaacccaa      780

ggcgaagcgu aucucgacaa acagucugac acccaguacg ucugcaaacg cacacucgug      840

gacagaggau ggggcaacgg augcggccuc uucggcaaag gcucccuagu aacaugcgcc      900

aaauucgcau gcuccaagaa aaugaccgga aaaagcauac agcccgaaaa ccucgaauac      960

cgcauaaugc uaucagucca cggaucccaa cacuccggca ugaucguaaa cgacaccggc     1020

cacgaaaccg acgaaaaccg cgcgaaaguc gaaaucaccc ccaacagccc acgagccgaa     1080

gccacacuag guggcuucgg aagccucgga cuggacugug aaccccgcac aggccuagac     1140

uucagcgacc uuuacuaccu caccaugaac aacaaacacu ggcucguaca caaagaaugg     1200

uuccacgaca uaccacuacc guggcacaca ggagcagaca ccggaacacc acacuggaac     1260

aacaaagaag ccuuaguaga guucaaagac gcccacgcaa aacgacaaac agucguagua     1320

cucggcucuc aagaaggagc cguccacacc gcacuagcag gcgcccucga agccgaaaug     1380

gacggagcua aaggcaggcu gagcucgggc caccuuaaau gcagacucaa aauggacaaa     1440

cuuagacuca aaggaguauc auauucccuc ugcacagcag cauucaccuu uaccaaaaua     1500

ccagccgaaa cccuacacgg aaccgugacc guagaaguuc aauacgcagg aaccgacggc     1560

ccaugcaaag uacccgcaca aauggcagug gacaugcaga cccugacacc ggucggacgc     1620

cucauaacag ccaaccccgu caucacugaa ggcacagaaa acucgaaaau gaugcucgaa     1680

cuagacccac ccuucggcga cuccuacaua gucaucggcg ucggugaaaa gaaaaucaca     1740

caccacuggc acagaagcgg cucaacaauc ggaaaagccu ucgaagccac cguccgcggu     1800

gcgaaaagaa uggcaguccu cggcgacaca gcaugggauu ucggaagcgu aggcggagua     1860

cuaaacucgc ucggcaaagg cauacaccaa aucuuuggcg cagccuucaa aagccuauuc     1920

ggaggcaugu ccugguucag ccagauccua aucggcacac uccuaaugug gcugggacuc     1980

aauaccaaaa acgggagcau aucccucaug ugccuugcac uaggcggcgu ucucaucuuc     2040

cucuccacag ccgucagcgc g                                               2061


<210>  2951
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short CDS opt

<400>  2951
accagcgucg guauagucgg cuuacuccuc acaacagcaa uggcagccga ggucacccga       60

agaggaucag ccuacuacau guaucuggac cgaucagacg caggggaagc aaucuccuuc      120

ccaacaaccc uaggcaugaa uaagugcuac auacaaauaa uggaccucgg acacaugugc      180

gacgccacga uguccuacga augcccaaug cuugacgagg gcguagaacc agacgacguc      240

gacuguuggu gcaacaccac guccacgugg gucgucuacg gaaccugcca ccauaagaaa      300

ggcgaagcgc gacguagcag acgagcaguc acucuaccaa gccacagcac cagaaaacua      360

caaacaagaa gccaaaccug gcuagaaagc cgagaguaca ccaaacaccu aauccgcgua      420

gaaaacugga ucuucaggaa cccuggcuuc gcacucgccg cagcagcaau cgccuggcug      480

cucggaucaa gcacaagcca aaaaguaaua uaccuuguga ugauccugcu aauagccccc      540

gccuacucca uacgcugcau aggagucuca aaucgcgacu ucguugaagg cauguccggc      600

ggcacauggg ucgacguagu gcucgaacac gguggcugcg ucacagucau ggcccaggac      660

aaaccaacag uagacauaga acuuguaacc acuaccguca guaacauggc cgagguacgu      720

ucauacuguu augaagcauc cauaucggac auggccucag acucccgaug cccaacccaa      780

ggcgaagcgu accucgacaa acaaucagac acacaauaug ucugcaaacg cacccuaguc      840

gacagaggcu ggggaaacgg augcggacuc uucggaaaag gcucccucgu aaccugcgca      900

aaauucgcgu gcucaaagaa aaugacgggc aaaagcauac aacccgaaaa ccucgaguac      960

cgcaucaugc uaucagucca cgguucacag cauagcggca ugauaguaaa cgacacagga     1020

cacgagacag acgagaaccg cgcaaaaguc gagauuacac cuaauagucc ccgcgcagaa     1080

gccacauuag gcggauucgg aagccuggga cuagacugcg aaccucgaac cggacucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaaacacu ggcuggucca caaagaaugg     1200

uuccacgaca uccccuuacc auggcacgcc ggcgcggaca caggcacccc acacuggaac     1260

aacaaagaag cccuagucga guucaaagac gcccacgcca aaagacaaac uguagucguc     1320

cuaggaagcc aagaaggagc agugcacacc gcacuggcag gcgcccuaga agcagaaaug     1380

gacggcgcca aaggcagacu gagcucaggc caccuaaaau gccgacucaa aauggauaaa     1440

cuccgauuaa aaggggucuc auacucccuc ugcaccgccg ccuucacuuu cacaaaaauc     1500

cccgccgaaa cccuccacgg uaccgucacc gucgaaguac aauacgccgg caccgacggc     1560

cccugcaaag uccccgccca aauggccgua gacaugcaaa ccuuaacccc agucggacgc     1620

cucaucacag caaaucccgu caucaccgaa uccaccgaaa acucgaaaau gaugcucgaa     1680

cuagacccac cauucggaga cagcuacauc gucaucggag uaggugaaaa gaaaauaacc     1740

caccacuggc accgcagcgg aagcaccaua ggaaaagccu ucgaggccac cguccgagga     1800

gcaaaaagaa uggcgguucu cggugacacc gcaugggacu ucggcagcgu aggcggagcu     1860

cugaacagcc uaggaaaagg aauccaccaa auuuucgggg ccgcauucaa aagccucuuc     1920

ggaggaaugu ccugguucuc gcaaauacuc auuggcacac uccucaugug gcucggacuc     1980

aacacaaaga acggcagcau cagccucaug ugccucgccc uugguggcgu ccucauauuc     2040

cucagcacag ccgucucagc c                                               2061


<210>  2952
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short CDS opt

<400>  2952
accagcgugg gcauagucgg acuccuacuc accaccgcca uggccgucga agucacgcgc       60

cggggcaacg ccuacuacau guaccuagac cguagcgacg caggagaggc aauaucauuc      120

cccacaacaa ugggaaugaa caaaugcuac auccagauca uggaccuagg ccacaugugc      180

gacgcaacaa ugucauacga augcccaaug cucgacgaag gaguggaacc cgacgacgua      240

gacugcuggu gcaacacaac aagcaccugg guaguauacg gaacgugcca ccacaagaaa      300

ggcgaagccc gacggucccg gcgagccguc acccucccaa gccacuccac acgaaaauua      360

caaacaagga gccaaaccug gcucgaaagc cgagaguaca ccaaacaccu caucagagug      420

gaaaacugga ucuuccgaaa cccuggauuc gcgcucgccg cagcagccau agcauggcuu      480

cucggcucca guaccagcca gaaaguuauc uaccucguaa ugauccuacu caucgcucca      540

gcauacucca uccgaugcau cggcgucagc aaccgcgauu ucgucgaagg uaugagcggc      600

ggcacauggg uagacgucgu acuagaacac ggcggcugug ugacagucau ggcucaggac      660

aaaccaacag ucgauaucga acucguaacc acaaccgucu ccaauauggc agaagucaga      720

ucauauugcu acgaagcaag caucuccgac auggccagcg acucacguug cccaacccaa      780

ggcgaagccu accuggacaa acaguccgac acccaauacg uaugcaaacg aacccucguu      840

gaccgaggcu ggggcaacgg cugcggccuc uucggcaaag gaucacucgu aacaugcgcc      900

aaauucgcuu gcucaaagaa aaugacggga aaaagcauac aacccgaaaa ccuagaguac      960

cguauaaugc ucuccgucca uggaucccaa cacucaggca ugaucgucaa cgacacgggc     1020

cacgaaacag acgaaaaccg cgcaaaaguc gaaauaacac ccaacagccc ccgagccgaa     1080

gcaacacucg gaggcuucgg uagccucggc cuagacugug aaccgcgcac aggacuagac     1140

uuuagcgacc ucuacuacuu aaccaugaac aacaaacacu ggcucgucca caaagaaugg     1200

uuccacgaca uaccauugcc cuggcacgca ggggccgaca caggcacccc acacuggaac     1260

aacaaagaag cccucguaga guucaaagau gcacacgcua aaagacaaac cgucgucgua     1320

cucggcagcc aagaaggcgc cguacacaca gcccuugcgg gcgcacuaga agcagaaaug     1380

gacggagcca aaggccgacu gucauccggc caccucaagu gcagacucaa aauggacaaa     1440

cuccgccuaa aaggcguuuc cuacagccuc ugcaccgccg ccuucacauu caccaagauu     1500

cccgcggaaa cucuccaugg gaccgucaca guagaaguac aauacgcagg aacagacggc     1560

cccugcaaag uccccgccca gauggccguc gacaugcaaa cccugacccc cgucgggcgu     1620

cuaaucacag ccaacccggu aaucaccgaa ucgaccgaaa acucaaaaau gaugcuagaa     1680

cuugacccgc ccuucggcga cuccuacauc gucauaggcg uaggcgaaaa gaaaauaacc     1740

caucacuggc accgauccgg uagcaccaua gggaaagccu ucgaagccac cguacgcgga     1800

gcaaaaagaa uggccguccu cggagacaca gcaugggacu ucggaucggu aggcggagca     1860

cucaauucac uaggcaaagg cauacaccag aucuucggcg cagcauucaa aagccucuuc     1920

ggcggaauga gcugguucag ccaaauccua auaggaaccc uacugguuug gcuaggccuc     1980

aacaccaaaa acggcucaau cagccucaug ugccuugccc uaggaggcgu ccucauauuu     2040

cuaucaacug cagucucagc a                                               2061


<210>  2953
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short CDS opt

<400>  2953
accuccguag gcauagucgg acuccucuug accacagcca uggcagccga agucacccga       60

cgcggcagcg cauacuacau guaccuagac cguuccgacg caggcgaagc aauuagcuuc      120

cccaccaccc uaggcaugaa caaaugcuac auacaaauaa uggaucucgg gcacaugugc      180

gaugccacaa ugucguacga augccccaug cucgacgaag gaguagaacc cgacgacguc      240

gacugcuggu gcaacacaac caguacaugg guagucuacg gaaccugcca ccacaagaaa      300

ggugaagcuc ggagaucccg acgcgcgguc acccuccccu cccacuccac aagaaaacug      360

caaacaaggu cacaaacaug gcucgaaagc agagaauaca ccaagcaccu aauaagagug      420

gaaaacugga uauuucggaa ccccggcuuc gcguuagccg cagccgccau cgcguggcuc      480

cuagggagcu caacuucaca aaaaguaaua uaccucguca ugauccuccu gauagcccca      540

gccuacucca uacguugcau aggcguaucu aaccgcgacu ucgucgaagg aauguccgga      600

ggcacauggg ucgacguggu acucgaacau ggcggcugcg uaaccguaau ggcacaagac      660

aaaccgacgg uagacauaga acugguuaca accaccguca gcaacauggc cgaaguaaga      720

ucauacuguu acgaagcauc aaucagcgac auggccagcg acucccgcug ccccacccaa      780

ggagaagcgu aucucgacaa gcaauccgac acccaauaug uaugcaaacg cacccucgug      840

gaccgaggau ggggcaacgg augcggccua uucggcaaag gaucccuagu cacaugcgcg      900

aaguucgcau gcucaaagaa aaugaccgga aaauccauac aacccgagaa ucucgaauac      960

agaaucaugc ucuccguaca cgggucucaa cauuccggca ugaucgugaa cgacacuggc     1020

cacgaaaccg augaaaaccg agcaaaaguc gagauaacac caaauucacc ccgcgccgaa     1080

gccacccuag gaggcuucgg aagccuaggc cucgacugcg aaccccgaac cggacuggac     1140

uucuccgacc uguacuaccu cacaaugaac aacaaacacu ggcuagucca caaagagugg     1200

uuccacgaca uaccacuucc cuggcacgcc ggcgccgaua cagggacacc ccacuggaac     1260

aacaaagaag cccuuguaga guucaaagac gcacacgcaa aacgccaaac agucgugguc     1320

cuaggcagcc aagagggagc cguacacacc gcccuagccg gagcccuaga agccgaaaug     1380

gacggcgcca aaggcagauu aagcuccggc caccucaagu gccgacuaaa aauggauaaa     1440

cugcgccuua aaggcguaag cuacucccuc uguacagcag cauucacauu cacaaaaaua     1500

cccgccgaga cacuccacgg aacaguaaca gucgaagucc aauacgcagg aacagaugga     1560

cccugcaaag ucccggcaca aauggccgua gacaugcaaa cccuaacccc cgucggcaga     1620

cucauaaccg caaaccccgu cauaacagag ucaaccgaaa acucaaaaau gaugcuggaa     1680

cucgacccac ccuucggcga cagcuacaua guaauaggcg uaggcgaaaa gaaaauaaca     1740

caccacuggc accgcagcgg aucaaccauc gggaaagccu ucgaagccac gguccgaggc     1800

gcaaaaagaa uggccgugcu cggcgacaca gccugggacu ucggcagcgu cggcggagcc     1860

cucaacuccc uaggcaaggg cauucaccaa aucuucggag ccgccuucaa aagccucuuc     1920

ggaggcaugu cgugguucuc ccaaauacua aucggcaccc uccuuaugug gcugggccuc     1980

aacaccaaaa acggcuccau cucgcuaguc ugccuagccc ucgggggagu ccucauauuc     2040

cuuucgacag caguaagcgc a                                               2061


<210>  2954
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short CDS opt

<400>  2954
accucaguug gaauaguggg gcuacuacua accacagcca uggcggcaga aguaacccga       60

agaggcagcg ccuacuacau guaccucgac cgcagcgacg ccggcgaagc aaucagcuuc      120

ccaaccaccc uaggaaugaa caaaugcuac auccaaauca uggaccuagg ucacaugugc      180

gacgccacca ugagcuacga augccccaug cuugacgaag gaguugaacc agacgacguc      240

gacugcuggu gcaacaccac aagcaccugg gucgucuacg ggaccugcca ccacaagaaa      300

ggcgaagcgc gccgaagccg cagagcuguc acccucccca gccacuccac ccgaaaacua      360

caaaccagga gccaaacaug gcucgaaagc cgcgaauaua ccaaacacuu aaucagagug      420

gagaacugga uauuccgcaa ccccggauuc gcacucgcag cggcagcgau cgcauggcuc      480

cuaggcagcu ccaccagcca aaaagucauc uacuuaguca ugauccuccu cauagccccc      540

gccuacucga ucagaugcau cggcguuaac aaccgagacu ucgucgaagg caugucuggc      600

ggcaccuggg uugacgucgu ccuagagcac ggcggcugcg uaaccguaau ggcucaagac      660

aaacccaccg ucgacaucga gcuagucaca accaccguaa gcaacauggc cgaaguucga      720

agcuacugcu acgaagcauc cauuagcgac auggcaucag acucaaggug cccaacacaa      780

ggagaggcuu acuuagacaa acaaagcgac acgcaauacg ugugcaaacg cacccuaguc      840

gaccgcggau ggggcaacgg cugcggccuc uucgguaagg gcagccuggu aaccugcgca      900

aaauucgccu gcucaaagaa aaugacagga aaaagcauac agcccgaaaa ucucgaauac      960

cguauaaugc uaagcgucca cggaucacaa cacagcggaa ugaucgucaa cgacaccgga     1020

cacgaaacag augaaaaccg agccaaaguc gaaaucaccc caaacagccc ccgcgcugaa     1080

gccacccugg gcggguucgg aucacuaggc cuagacugcg agccgcgcac aggccuggac     1140

uucagcgacc ucuacuaccu aaccaugaac aacaaacacu ggcucguaca caaagaaugg     1200

uuccacgaca ucccacuccc auggcacgcc ggcgcagaca ccggaacccc gcacuggaac     1260

aacaaagaag cacucgucga guucaaagac gcccacgcca aacgccagac agucgucguc     1320

cugggauccc aagaaggcgc cguccacaca gcacucgccg gcgcccuaga ggccgaaaug     1380

gacggagcaa aaggacgccu aucaaguggc caucucaaau gccggcuuaa aauggacaaa     1440

cuuagauuga aaggcgucuc cuacucucuc uguaccgcgg ccuucacauu cacaaaaaua     1500

cccgcggaaa cacuccacgg cacagucacc gucgaggucc aguacgcagg gaccgauggg     1560

ccgugcaaag uaccagccca aauggccgua gacaugcaaa cacuaacccc cgucggacgu     1620

cuaauaacag ccaaccccgu cauaacggaa ucaacagaga acuccaaaau gauguuagaa     1680

uuagacccuc cauucggcga cucguacauc gucauagggg uaggagaaaa gaaaaucaca     1740

caccacuggc accgcagcgg aagcaccaua ggcaaagcgu ucgaagccac cguacgaggc     1800

gccaaacgca uggccguccu cggagauacc gccugggacu ucggaagcgu cggcggagcc     1860

cucaacucuc uaggaaaagg cauccaccaa auauucggag ccgcauucaa aagccuauuc     1920

ggaggcaugu caugguucuc ccaaauacuc aucggaacgc ucuuaaugug gcuaggccuc     1980

aauaccaaaa acggcuccau aucccuaguc ugcuuagccc ucggcggcgu ccuaauauuc     2040

cuaagcaccg caguaagcgc a                                               2061


<210>  2955
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short CDS opt

<400>  2955
acaucaguag ggaucgucgg ucugcuccug accacagcaa uggcagugga agucacacga       60

cgcggcagcg cguacuacau guaccucgac cgaucagacg caggcgaagc aaucuccuuc      120

cccaccacac ucggcaugaa caaaugcuac auccaaauca uggaccuagg acacaugugc      180

gacgcaacaa ugucauacga augcccaaug cuggacgaag gaguagaacc cgacgacguc      240

gacugcuggu gcaacacuac cagcacaugg gucguauacg ggacuugcca ucacaagaaa      300

ggggaagcaa gacgcucacg acgagcgguc acgcucccga gccacucaac acggaaacuc      360

caaacucggu cgcaaaccug gcuagaauca agagaguaca caaaacaccu aauacgagua      420

gaaaacugga ucuucagaaa cccaggcuuc gcccucgccg cggccgccau cgccuggcuc      480

cuaggcagca gcaccuccca aaaagucaua uaccugguaa ugauccuacu gauagcacca      540

gccuacucaa ucaggugcau aggaguaagc aaccgagauu ucguagaagg aauguccgga      600

ggcacauggg uagacguggu ccuggaacac ggcggaugcg ugacagucau ggcccaagac      660

aaaccagccg ucgauauaga acucgucacc acaaccguaa gcaacauggc agaaguaaga      720

uccuacugcu acgaagccag cauaagugac auggcaagcg acagccgcug ccccacccaa      780

ggcgaagccu accucgacaa acaaucagac acccaguacg uaugcaaacg cacauuagua      840

gacagaggcu ggggcaacgg cugcggacua uucgggaaag gcucgcuggu aaccugcgcc      900

aaauucgcgu gcuccaagaa aaugaccggc aaaagcauac agcccgaaaa ccucgaauac      960

cggauaaugu uguccgugca cggcagccaa cacuccggca ugaucgucaa cgacaccgga     1020

caugagacag acgaaaaccg cgccaaagua gaaauaaccc cuaacagccc gcgagccgag     1080

gcgacccucg gagguuucgg cucguuaggc cucgauugcg aaccccguac cggacuagac     1140

uucuccgacu uauacuaccu cacgaugaac aacaaacacu ggcuuguaca caaagaaugg     1200

uuccacgaca uaccacugcc cuggcacgcc ggcgcagaca caggcacccc acacuggaac     1260

aacaaagagg cccuaguaga auuuaaagac gcccaugcaa aacgccaaac aguaguaguc     1320

cucggcagcc aagaaggcgc agugcacaca gcacuagccg gagcccuaga ggccgaaaug     1380

gacggcgcaa aagggcgacu aaguagcgga caccuaaaau gccggcucaa aauggacaaa     1440

cuccgccuca aaggcgucuc cuacucccua ugcaccgcag cauuuacauu cacaaaaauc     1500

cccgcagaaa cccuacaugg aaccgugaca guagaagucc aauacgcagg aacggacggg     1560

ccaugcaaag ugcccgcaca aauggcagua gacaugcaaa cacucacacc agucggcaga     1620

cuaaucaccg cgaaucccgu gauaaccgaa ucaaccgaaa acagcaaaau gaugcuagaa     1680

cuagacccac ccuucggcga caguuacaua gucauaggcg uaggagaaaa gaaaaucaca     1740

caccacuggc accgauccgg aucaacaaua ggaaaagcgu ucgaagcgac cguacgcgga     1800

gccaaacgaa uggcaguucu cggagacaca gccugggacu ucggaagcgu aggcggcgcg     1860

cuaaacagcc ucggcaaagg cauccaucag aucuucggag cagcguucaa aucacuauuc     1920

ggagggauga gcugguucag ucagauacua auaggcacau uguuagugug gcucggccua     1980

aacacgaaaa acggcagcau cagcuuaacc ugccucgcac ucggcggcgu acucauuuuu     2040

cuaagcaccg caguaucagc c                                               2061


<210>  2956
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short CDS opt

<400>  2956
acaucagucg gcaucguagg ccuccuucua acaaccgcaa uggccguaga gguaacgcgu       60

cgaggcucag cguacuacau guaccuggac cgaucagacg cgggggaggc gauaucguuc      120

ccaaccacgc ucggaaugaa caaaugcuac auacaaauaa uggacuuagg acacaugugc      180

gacgccacca uguccuacga augccccaug cucgacgaag gaguggaacc agacgacguc      240

gacugcuggu gcaacacaac caguaccugg guagucuacg gcacgugcca ccacaagaaa      300

ggcgaagccc gccgcucccg ccgagcuguc acacucccaa gccauagcac acgcaaacug      360

cagaccagaa gccaaaccug gcucgaaucu agagaauaua caaaacaccu gauccgcgua      420

gagaacugga uauucagaaa cccaggauuc gcccuagccg cagcagucau cgccuggcua      480

cucggaucca gcacaucaca gaagguaaua uaccucguaa ugauauuacu caucgcacca      540

gcguauucga uccggugcau cggcguaagc aaccgagacu ucguugaagg aaugucaggc      600

gggaccuggg ucgacgucgu ccuggaacac ggaggaugcg uuacggucau ggcacaagac      660

aaacccacag ucgacaucga acucgucaca acgaccguaa guaacauggc agaaguacgg      720

uccuacugcu acgaagcuag cauaucagac auggcuucag acuccagaug ccccacacag      780

ggcgaagccu accuagacaa acaaucagac acgcaauacg ucugcaaacg aacccucgua      840

gaccgaggcu ggggaaacgg cugcggccuc uucggcaaag gaucgcucgu gaccugcgcc      900

aaguuugcau gcuccaagaa aaugacaggc aaauccauac aacccgagaa ccucgaauac      960

cgcaucaugc ucagcgucca cggaucacaa cacucgggaa ugauagucaa cgacaccggg     1020

cacgagaccg acgaaaaccg cgccaaggug gaaauaacac cuaacagucc cagagcagaa     1080

gccacacucg gcggauucgg gucacucggc cucgacugcg aaccacgaac cggacuagac     1140

uucagcgacc ucuacuaccu gacaaugaac aacaaacacu ggcuuguaca caaagaaugg     1200

uuccacgaua uaccacuccc auggcacgcc ggggccgaua ccgguacccc acacuggaac     1260

aacaaagagg cacucguaga guucaaagac gcacacgcaa aacgccaaac cgucgucgua     1320

cucgggucac aagaaggcgc cgugcauaca gcccucgcug gcgcccuaga agccgagaug     1380

gacggcgcua agggucgacu uuccucaggc caccuaaaau gccgccugaa aauggacaaa     1440

cuccggcuca aaggggucuc cuacucacuc ugcaccgcag ccuucacuuu caccaagaua     1500

cccgccgaaa cccuccacgg caccgucacc gucgaagucc aauacgcagg aaccgacgga     1560

cccugcaaag uacccgcuca aauggcggua gacaugcaaa cccugacgcc cgucggccgc     1620

cuaauuaccg ccaauccugu cauaaccgaa ucgacagaaa acagcaaaau gaugcucgaa     1680

cuggacccac ccuucggcga cagcuacaua gucaucggcg ucggcgaaaa gaaaaucacc     1740

caccacuggc accgauccgg uagcaccaua ggaaaagcau ucgaagccac cguacgggga     1800

gcaaaaagaa uggcgguacu gggcgacacc gcaugggacu ucgggagugu cggaggagcc     1860

cuuaacucgu uaggcaaagg cauacaccag auauucggcg ccgccuucaa aagccuguuc     1920

ggcggcauga gcugguucag ccaaauccua aucgggacac uccuggucug gcuaggccuc     1980

aacaccaaga acggauccau uagccuaacc ugccuggcac ucggaggagu ucuaauauuc     2040

cucagcaccg ccgucagcgc a                                               2061


<210>  2957
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short CDS opt

<400>  2957
acgagcguag gcauaguagg ccuccuacua accaccgcaa uggccguaga gguaacccgc       60

cgaggcucag ccuacuacau guaccuagac cgaagcgacg ccggcgaagc caucagcuuu      120

cccacgaccc uaggagucaa caaaugcuac auacaaauaa uggaccucgg gcacaugugc      180

gacgcaacca ugucauacga gugcccaaug cuagacgaag gagucgaacc cgaugacguc      240

gauugcuggu guaacaccac cucaacgugg gucgucuacg gaacaugcca ccacaagaaa      300

ggcgaagcac ggcggagcag acgagcaguc acgcuaccau cccacucaac acgcaaacuc      360

caaacccgau cacaaacaug gcucgaaucc cgcgaauaca ccaaacaccu aaucagagua      420

gaaaacugga ucuuucgcaa ccccgguuuc gcacucgcag cagccgcuau cgcauggcua      480

cuaggcucau ccaccucaca gaaagucauu uaccucguga ugauccuacu aaucgcaccc      540

gcauacucga uccgcugcau aggaguuagc aaccgcgacu ucguagaagg aaugagcggc      600

ggcacauggg uagacguagu gcuagaacac ggcggcugcg ucaccgucau ggcccaggac      660

aaacccacgg uagacaucga auuagucacu acaacgguca gcaacauggc agaaguccgc      720

ucauacugcu acgaggcaag caucuccgac auggcuuccg acagccgcug ucccacgcag      780

ggcgaagccu accuagacaa acaauccgac acacaauacg ucugcaaaag gacccuagua      840

gacagaggau ggggaaacgg gugcggacuc uucggaaaag gcagccucgu gaccugcgcc      900

aaauucgccu gcucaaagaa aaugaccgga aaaagcaucc aacccgaaaa ucucgaguac      960

agaaucaugc ucagcgucca cggaucacaa cacagcggca ugauagucaa cgacacgggg     1020

cacgaaacgg augaaaaccg cgccaaaguc gaaaucaccc ccaacagccc ccgagccgaa     1080

gccacccucg gcggguucgg aucgcuagga cucgacugcg aaccacgaac aggccuggac     1140

uucagcgacc ucuacuaccu aacgaugaac aauaaacacu ggcuaguaca caaagaaugg     1200

uuucacgaca ucccacuccc cuggcacgcc ggagccgaca ccggaacacc ccacuggaac     1260

aacaaagaag cacugguuga guucaaggac gcacacgcaa aacgccagac cgugguugug     1320

cuaggcucac aagaaggcgc aguacacacc gcccuggcag gagcucuaga agccgagaug     1380

gacggagcca aagggcgccu cagcuccgga caccucaaau gccgccuaaa aauggacaaa     1440

cuccgacuaa aaggagucuc uuacucacuc ugcaccgccg cauucaccuu caccaaaaua     1500

cccgcagaga cccuccacgg cacagugacc gucgaaguac aauacgccgg aacagacggc     1560

ccaugcaaag uaccggccca aauggccgua gacaugcaaa cacucacccc agucggcaga     1620

cuaaucacag caaaccccgu cauaacagaa uccacagaga acagcaaaau gaugcucgaa     1680

cuagauccgc ccuucggcga uagcuacaua guaaucggug ucggagacaa gaagaucacc     1740

caccacuggu accgauccgg uagcaccauc ggcaaagcau ucgaagccac cguccgaggc     1800

gccaaaagaa uggcaguccu cggcgacacc gcgugggacu ucggcagcgu aggaggagcc     1860

cucaacuccc ucggaaaagg cauccaccaa aucuucggcg ccgccuucaa aagccuauuc     1920

gguggaauga gcugguucuc ccagauccug auuggcacgc uucuaguaug gcuaggccua     1980

aacacgaaga acggcagcau aucccucacg ugccuugcgc uaggcggcgu acuaauauuc     2040

uuaagcaccg ccguaagcgc a                                               2061


<210>  2958
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short CDS opt

<400>  2958
accagcgucg gcaucguggg ccuccuccua acuaccgcca uggccgucga agucacacgc       60

cgcggcagcg cguauuacau guaccuggac agaagcgaug ccggaaaagc cauaucauuc      120

cccaccaccc uaggaguuaa uaaaugcuac auacaaauca uggaccucgg ccacaugugc      180

gacgcaacca uguccuacga augcccaaug cuagacgaag gcguagaacc ugacgacgug      240

gacugcuggu gcaacaccac aucaaccugg gucguuuacg gcacaugcca ccacaagaaa      300

ggggaagccc gacgcuccag gcgcgcgguc acccucccau cacacucaac ccgcaaacua      360

caaacacgau cccagacuug gcuagaaucg cgcgaauaca ccaaacaccu caucagaguc      420

gagaacugga ucuuccgaaa ccccggcuuc gcacuagcag ccgcugccau agcaugguua      480

uuaggcucga gcaccucaca gaaagugaua uaucucguca ugauacugcu caucgcgcca      540

gcguacucca uucggugcau aggcguaagc aacagagacu ucgucgaagg cauguccggc      600

ggaaccuggg uagacgucgu ccucgaacac gguggcugcg ugaccguaau ggcccaagac      660

aaacccacag uagacaucga acugguuacc acaaccguau ccaacauggc cgaagugcgc      720

ucauauugcu augaagccag caucucagac auggcaucgg acucccgcug cccaacacaa      780

ggagaagcgu accucgacaa acaaagcgac acccaguacg ucugcaaaag gacacucguc      840

gacagaggcu ggggcaacgg augcggccuc uucggcaaag gcagccucgu aaccugcgcc      900

aaauuugccu guagcaagaa aaugaccggc aaaagcauac aacccgaaaa ccucgaauac      960

cggauuaugc ucagcgucca cggcucacaa cacagcggca ugaucgucaa ugacauaggc     1020

cacgagaccg acgaaaaccg cgccaaaguc gagaucaccc ccaacucccc acgcgcagag     1080

gcaacucucg ggggauucgg cucccucggc uuagacugcg aaccccgaac cggacucgac     1140

uucucagacc uguacuaccu caccaugaau aacaaacacu ggcuagucca caaagaaugg     1200

uuccacgaca uaccccuccc cuggcacgca ggcgcggaca ccggaacacc gcacuggaac     1260

aacaaagagg cccucgucga guucaaagac gcacacgcca aacgucaaac aguagucgua     1320

cucggcuccc aagaaggcgc cguacauacc gcacucgccg gcgcccuaga agcagaaaug     1380

gacggcgcca aaggacgucu aagcuccggc caccucaaau gcagacugaa aauggauaaa     1440

cuacgccuca aaggcgucag cuacucccuc ugcacagccg cuuucacauu uaccaaaauc     1500

ccagccgaaa cauuacacgg aacuguuacc guagaagucc aguacgccgg caccgacggc     1560

ccaugcaaag ucccagccca aauggcaguc gauaugcaaa cacuuacacc agucggacgc     1620

cucaucacag caaacccggu aauaaccgaa ucaaccgaaa acuccaaaau gaugcuagag     1680

cucgaucccc cuuuuggcga cagcuacauc gucaucggag ucggggacaa gaaaaucacc     1740

caccacuggc accgcucagg cuccaccauc gggaaagcau ucgaagccac aguacguggu     1800

gccaaacgca uggccguacu cggcgacaca gcgugggacu ucggcagcgu cggaggcgca     1860

cucaacucac ucgggaaagg cauccaccaa aucuucggcg ccgcauucaa aucccuauuc     1920

ggcggcaugu caugguucuc ccaaauacua aucggcaccc uacucgucug gcuaggacuc     1980

aacaccaaaa acggaagcau cagccucacg ugccuagccc ucggaggcgu ccucauauuc     2040

cucuccaccg ccgucuccgc c                                               2061


<210>  2959
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short CDS opt

<400>  2959
acaagcaucg gcaucgucgg gcuccuacuc accaccgcaa uggccgcaga aaucacccga       60

agaggaagcg ccuacuacau guaccuggac cggagcgacg caggaaaagc cauuagcuuc      120

gccaccacac uaggaguaaa caaaugucac gugcagauca uggaccuagg ccacaugugu      180

gacgcgacaa uguccuacga augcccaaug cuggaugaag gaguagaacc cgacgacgug      240

gacugcuggu gcaacacaac uagcacaugg gucgucuacg gcacaugcca ccacaagaaa      300

ggagaggccc gaagaucccg gcgcgcaguc acccuaccgu cccacaguac ccgcaaacuc      360

caaaccagaa gccaaacaug gcuagaaagc agagaauaca cgaaacaccu caucaaaguu      420

gaaaacugga ucuuccgaaa ccccggauuc acgcugguag cagucgcaau cgcauggcuc      480

cuagggucua gcacuucaca aaaaguaaua uaccucguca ugauccuucu gauagcgccg      540

gccuacagca uacgcugcau cggaguaucc aauagagacu ucguagaggg gauguccggc      600

ggcacauggg ucgacguagu ccucgaacau ggaggcugug ucaccguaau ggcccaggac      660

aagccaaccg uagacaucga gcucguaaca accaccguau ccaacauggc ggaaguaaga      720

agcuacugcu acgaagccag caucagcgac auggccuccg acucgcggug cccaacccaa      780

ggcgaagccu aucucgacaa gcaauccgac acacaguacg uaugcaaaag aacccucguc      840

gaucgcggcu ggggcaacgg gugcggccuc uucggaaaag gcagccucgu aaccugcgca      900

aaauuuaccu gcuccaaaaa gaugaccgga aaaucaaucc agccagaaaa ccucgaauac      960

cgcaucaugc ucagcgucca cggaagccaa cauagcggca ugaucguaaa cgacacagga     1020

cacgaaaccg acgaaaaccg cgcaaaggua gaaguaaccc ccaacagccc acgcgcggaa     1080

gcgacgcuag gaggcuucgg aagccuggga cucgacugcg aaccccgcac gggccuggac     1140

uucucagacc ucuacuaccu cacaaugaac aacaagcacu ggcuagucca caaagaaugg     1200

uuccacgaca ucccacuccc guggcacgcg ggcgcagaca ccggaacacc ccacuggaac     1260

aacaaagagg cccuagugga guucaaagac gcacacgcca aacgacaaac cguagucguc     1320

cuaggcagcc aagaaggcgc cguccacacc gcccucgcag gcgcccuaga agccgaaaug     1380

gacggagcaa aaggacgacu auucucggga caccucaaau gcagacucaa aauggacaaa     1440

cuuagacuaa aaggggucuc auacagccuc uguacagcag cauucaccuu cacgaaagua     1500

ccggccgaaa cacuacacgg aaccguaaca guagagguac aauacgcagg gacggacggc     1560

ccgugcaaag ucccagccca aauggcugua gacaugcaaa cccuaacacc agucggccgc     1620

cucauaaccg caaacccagu uaucacagaa ucaacagaaa acucaaaaau gaugcuagaa     1680

cuagacccac ccuucggaga cagcuacaua gucauaggag uuggagacaa gaaaauaaca     1740

caccacuggc accggucagg gagcacaauc ggaaaagccu ucgaagcaac cguaagaggc     1800

gcaaaaagaa uggcgguccu uggcgacacc gcaugggacu ucggaucagu uggaggcgug     1860

uucaacucac ucgggaaagg cauacaccaa auauucggag cggcauucaa aucacuauuc     1920

gggggaaugu ccugguucag ucaaauacuc auaggcaccc ugcuaguaug gcuugggcua     1980

aacacaaaga acggauccau aagccuaacc ugccucgcac ugggaggcgu caugaucuuc     2040

cuaucaaccg ccgugagcgc c                                               2061


<210>  2960
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short CDS opt

<400>  2960
acaucaauag ggaucauagg ccuccuccug acaaccgcaa uggcggcuga gauaacgcgc       60

cgagggagcg ccuacuacau guaccuagac agaucggacg cagggaaagc aaucagcuuc      120

gccaccaacu ugggagucaa caaaugccac guccaaauca uggaccucgg acacaugugc      180

gacgccacaa ugucuuacga gugccccaug cuagacgaag ggguagaacc cgacgacgug      240

gacuguuggu gcaacaccac aagcaccugg guagucuacg gaaccugcca ccacaagaaa      300

ggcgaagccc ggagaucccg ccgagccgua acccuccccu cccacucgac ccgaaaacuc      360

cagacaagaa gucaaaccug gcuugaaucc agagaauaca cgaaacaccu aauaaaaguu      420

gaaaacugga ucuuccgcaa ccccggcuuc gcccucgccg cggucgccau agccuggcuc      480

cuaggcagcu cgaccaguca aaaaguaauc uaccuaguca ugauccuacu caucgccccg      540

gcauacagca uacgauguau cggagugagc aaccgagacu ucgucgaagg aaugagcggc      600

ggaacuuggg uagacguugu cuuagagcac ggcggcugcg uaaccgucau ggcccaggac      660

aaaccaaccg ucgacaucga gcuagugacc accacggucu caaacauggc cgaagugaga      720

ucguacugcu acgaagccuc cauauccgac auggcaucag acucgagaug cccaacacaa      780

ggcgaagccu accucgacaa acaaagcgac acacaauacg ugugcaaacg cacccuagua      840

gacagaggau ggggcaacgg augcggacuc uucggaaaag gcagccuagu cacuugcgcg      900

aaauucacau gcagcaagaa aaugaccggc aaaagcauac aaccggaaaa ccuagaauac      960

cgcaucaugc ucagcguaca cgguucacaa cacuccggca ugaucgucaa cgacauagga     1020

cacgaaaccg acgaaaaccg cgcaaaagug gaagucaccc caaacucacc gagagcagaa     1080

gcaacacucg gcggauucgg aucacuaggu cucgacugcg aaccgcgcac uggccuagac     1140

uucuccgacc ucuacuaccu uacaaugaac aacaagcacu ggcuagucca caaagaaugg     1200

uuccaugaca uuccguugcc auggcacgca ggagccgaca caggaacacc acacuggaac     1260

aacaaagaag cgcuaguaga guucaaagac gcccacgcca agcgucaaac cgugguaguc     1320

cuaggaagcc aagaaggcgc cgugcacaca gcacuagccg gagcacucga agccgaaaug     1380

gacggugcca aagggcgacu auucagcggc caccucaaau gcaggcucaa aauggacaaa     1440

cugcgacuaa aaggaguauc guacucacua ugcaccgccg ccuucacauu cacaaaagua     1500

ccggccgaaa cccuacacgg cacaguaacc guggaagucc aguacgcagg aaccgacgga     1560

ccuugcaaag uccccgccca aauggccguc gacaugcaaa cacucacacc cgucggcaga     1620

cugauaaccg caaacccagu aaucaccgaa agcacugaga acagcaaaau gauguuagaa     1680

cuagaucccc cauucggcga cucauacauc gucaucggag ucggcgacaa gaaaauaacc     1740

caccacuggc accgcagugg cucaaccauc ggcaaagcau ucgaagccac cguacgcggc     1800

gccaaacgca uggcaguccu cggugacaca gcaugggauu ucggaagcgu cggcggcguc     1860

uucaacuccc ucggcaaagg ugugcaccaa aucuucggcg ccgccuucaa gagccucuuc     1920

ggaggcaugu cgugguucuc acaaauccuu aucgggacac uccuagucug gcuaggacua     1980

aacaccaaga acggcaguau cagccuaacc ugccucgccc uaggcggggu aaugauauuc     2040

cuaagcacgg ccguaagcgc a                                               2061


<210>  2961
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short CDS opt

<400>  2961
accuccaucg ggaucauagg ccuauuacua accaccgcaa uggcagccga gaucacccgg       60

cgcgguagcg ccuacuacau guaccucgau agaucagaug caggcaaagc aauauccuuc      120

gcaaccacac ucggaguaaa caagugccac guccaaauca uggaccuagg ccacaugugc      180

gacgcaacaa ugucauacga augcccaaug cuagacgagg gcgucgaacc cgacgacgua      240

gacugcuggu gcaacaccac aagcacaugg guagucuacg gaacauguca ccacaagaaa      300

ggagaggcca ggagaagccg acgcgcaguc acccucccaa gccacucgac acgaaaacuc      360

caaacacgca gccaaacaug gcuggaaagc cgcgaauaca ccaaacaccu caucaaagua      420

gaaaacugga ucuuccgcaa cccaggcuuc gcacucguag ccguagccau agccuggcuc      480

cugggaagca gcaccagcca aaaagucaua uaccucguaa ugauccuccu gaucgcacca      540

gccuacucga ucaggugcau uggcgucagc aaccgagacu ucgucgaagg cauguccgga      600

ggcacauggg uagacgucgu gcuagagcac ggcggcugcg ucacggucau ggcccaagac      660

aaacccacgg uggacauuga acucguaacc accacaguca gcaacauggc ugaagucaga      720

agcuacugcu acgaggcauc gaucagcgac auggccagug acucaagaug cccaacacaa      780

ggggaagcau accucgacaa acagucagac acccaauacg ucugcaagcg aacacucgua      840

gaccgcggcu ggggcaacgg cugcggacuc uucggcaagg gcucucuggu cacaugcgcc      900

aaauucacgu guagcaagaa aaugacuggc aaaucaaucc aaccagaaaa ucucgaauac      960

cgaaucaugc uauccguaca cggcucacaa cacagcggca ugauaguaaa ugacauaggu     1020

caugaaacug acgaaaacag agcaaaaguc gagguaaccc ccaacagccc gcgcgcugaa     1080

gcaacgcucg gcggcuucgg gagccuagga cucgacugcg aaccccgaac gggccuagac     1140

uuuagcgauc uguacuaccu cacaaugaac aacaaacacu ggcucgugca caaagagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggagccgaca ccggcacacc ccauuggaac     1260

aacaaagaag cccucgugga guucaaggac gcacacgcaa aacggcaaac cguagucguc     1320

cuaggaucac aggaaggcgc cguccacacc gccuuagcgg gagcacucga agcagaaaug     1380

gacggcgcca aagggcgccu auucuccgga caccugaagu gucgucucaa aauggacaaa     1440

cuccgacuaa aaggaguguc cuacagccuc ugcaccgcag cauucacauu caccaaaguc     1500

cccgccgaaa cccuccacgg gacaguaaca gucgaagucc aauacgccgg uacagacgga     1560

ccaugcaaag uccccgccca aauggcagua gacaugcaaa cacuaacccc cgucgggcga     1620

cucaucaccg ccaaccccgu uauaacagaa agcacggaaa acagcaaaau gaugcuggaa     1680

cuugacccuc ccuucgggga cucauacaua guaauaggcg ucggagacaa gaaaaucaca     1740

caccauuggc accgaagcgg auccacaaua ggaaaagcau ucgaagccac cguacgaggg     1800

gcaaaacgga uggcaguacu aggcgacacc gccugggacu uuggcucagu aggcggcguc     1860

uucaacagcc ucgggaaagg cauccaccaa auauucggug ccgccuucaa aucacucuuc     1920

ggcggaauga gcugguucag ccaaauccug auuggaaccc uacucgucug gcugggcuua     1980

aacaccaaaa acggcagcau cucccugaca ugucucgccc uagggggagu aaugauauuc     2040

cuaucaaccg ccgucagcgc a                                               2061


<210>  2962
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short CDS opt

<400>  2962
acaagcauag guaucauagg gcuucuacuc acaaccgcca uggcagccga aaucacccga       60

cggggcuccg ccuacuacau guacuuagac agaagcgacg caggaaaagc caucucauuc      120

gccaccaacc uaggagucaa caagugccac guacaaauaa uggaccuggg acacaugugc      180

gacgccacua uguccuacga augccccaug cucgacgagg gcgucgaacc agacgacguu      240

gacugcuggu gcaacaccac aagcacaugg gucgucuacg ggaccugcca ccacaagaaa      300

ggcgaagcac gccggagccg gcgcgccguc acgcuuccga gccacagcac ccgcaaacua      360

caaacaagau cacagacgug gcucgagagc agagaauaca ccaagcaccu caucaaagua      420

gaaaacugga uuuuccgcaa ccccggauuc gcucuagccg cagucgcaau cgccuggcuc      480

cuaggcucga gcacaagcca aaaagucauc uaccuaguaa ugauccuccu aaucgcaccc      540

gcauacucaa uacgcugcau aggcgucagc aaccgcgacu uuguagaagg aaugucagga      600

gggacauggg uagacguagu ccucgaacac ggaggcugcg ucaccgucau ggcacaggac      660

aaacccaccg uagacauaga gcucguaacc acaacaguaa gcaauauggc ggaaguccgu      720

uccuacuguu acgaggcuuc aaucucagac auggcgucag acucacgaug ucccacccaa      780

ggcgaagcau accuggacaa acaaucagac acccaauacg uauguaaaag gacccucguu      840

gaccgcggcu gggggaacgg augcgggcua uucggaaaag gaucccucgu caccugugcc      900

aaauucacau gcucaaagaa aaugaccgga aaaucaaucc agccggaaaa ccuagaauac      960

aggauaaugc ucucagucca cggaagccaa cacagcggaa ugauagucaa cgacgaaaac     1020

agggccaaag ucgagguuac acccaacagc ccacgcgccg aagcaacacu aggaggauuc     1080

gggagccuag gucuugacug cgaaccgcga accggccugg acuucucaga ccucuacuac     1140

cuaaccauga acaacaaaca cuggcucgua cacaaagaau gguuccacga caucccgcuc     1200

ccguggcacg ccggagcaga cacaggcacc ccacacugga acaacaaaga ggcccucgua     1260

gaguucaaag acgcccacgc gaaacgccaa acaguaguag uacuggguuc ccaagaaggc     1320

gcaguccaca ccgcgcuagc aggcgcacua gaagccgaaa uggacggagc caaaggacgu     1380

cuauucuccg gacaccucaa augccggcua aaaauggaca aacuaagacu caaaggagua     1440

ucguacuccc ucugcacagc cgccuuuaca uucacgaagg ucccagccga aacccuacac     1500

ggaaccguga ccgucgaagu acaauaugcg ggaaccgacg gcccaugcaa gguaccagcc     1560

caaauggcag ucgacaugca aacccucacg cccgucggac gccucaucac agcaaaucca     1620

guaaucacag aaagcaccga aaauucaaaa augaugcugg aacuagaccc acccuucgga     1680

gacagcuaca ucguaaucgg cgucggagac aagaaaauca cacaucacug gcauaggagc     1740

gggagcacaa uuggaaaagc cuucgaagca accguaagag gcgccaaacg aauggcgguc     1800

cuaggugaca cagccuggga cuucggcagc gucgggggcg ucuucaacag ccuaggcaaa     1860

ggcauccacc aaauauucgg agcagccuuu aaaagccuau ucgguggcau gucaugguuc     1920

agccagaucc ucauaggaac ccugcuaguc uggcuaggac uaaacacgaa aaacgggagc     1980

auaagccuga caugccucgc ucugggaggc gucaugauau ucuuaagcac ggccguaagc     2040

gca                                                                   2043


<210>  2963
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short CDS opt

<400>  2963
accagcauag gaauaguagg ccuccuacuc acaaccgcca uggcggcgga gaucaccaga       60

cgaggcagcg cauacuacau guaccucgac aggucagacg ccgggaaagc aaucagcuuc      120

gcaaccacac ugggagucaa caaaugccac guacagauaa uggaccuggg acacaugugu      180

gacgcaacca ugucauacga gugccccaug cuagaugaag gaguagagcc cgacgacgua      240

gacugcuggu gcaauacaac cagcacaugg gucguguacg gaaccugcca ccacaagaaa      300

ggcgaagcua gaagaagccg cagagcggua acccuaccaa gccacagcac gcgaaaacuc      360

caaacccgaa gccaaaccug gcuugagagc cgggaauaca cgaaacaccu aaucaaggua      420

gaaaacugga uauuccgcaa ucccgguuuc gcgcucgucg cagucgcaau agcauggcuc      480

cuaggaagca gcacaagcca gaaagugaua uaccuaguaa ugauccuacu gauagcccca      540

gcguacagca uccgcugcau cggcguaagc aaccgcgacu ucguagaagg aauguccgga      600

ggcacauggg uagacguagu ccucgagcac ggcggcugug uaacaguaau ggcacaagac      660

aaaccaacgg ucgacauaga gcuagucacc acaacaguaa gcaacauggc agaagugcga      720

agcuacugcu acgaagcauc caucucagac auggccagcg acagucgaug ccccacacaa      780

ggcgaagcau accuagacaa acaauccgac acccaauacg ucugcaaaag aacgcuaguc      840

gauagagguu ggggcaacgg cugcggauua uucggcaaag gcucgcuagu gacaugcgcg      900

aaauucacau gcagcaagaa aaugacgggc aaaagcaucc agcccgaaaa ccucgaguac      960

agaauaaugc uauccgucca cggcagccaa cacagcggca ugauagucaa cgacacuggg     1020

cacgaaacag acgaaaacag ggccaaagua gaagucaccc caaacagccc acgcgcggaa     1080

gcgacccucg gaggguucgg gucacucgga cuagacugcg aaccacgaac agggcucgac     1140

uucuccgacc ucuacuaccu aacgaugaac aacaaacacu ggcugguaca caaagagugg     1200

uuccacgaca uacccuugcc auggcacgca ggggcggaca caggaacccc acacuggaac     1260

aauaaagagg cgcuagucga guucaaagac gcacacgcaa aacgacaaac aguaguagua     1320

cuaggaagcc aagaaggcgc aguacacaca gcacuagccg gcgcgcugga agccgaaaug     1380

gacggcgcaa aaggacgacu auucagcggc caccugaaau gcaggcucaa aauggacaaa     1440

cugagacuga aaggagucuc cuacucacua ugcacagcag cauuuaccuu cacgaaagua     1500

ccagccgaaa cccuacacgg aacaguaacg guagaagucc aguacgcagg aacagacggg     1560

ccaugcaaag uaccagcaca gauggccguc gacaugcaga cccucacacc agucgggcgc     1620

cuaauuacag ccaacccggu aaucacagaa agcaccgaaa acuccaagau gaugcuagaa     1680

cuagacccac ccuucggcga cagcuacauc gugaucggug uaggugacaa gaaaaucaca     1740

caccacuggc accggagcgg cagcacaaua gggaaagccu ucgaagcgac aguacgcggc     1800

gcgaaacgca uggccguacu cggcgacacc gcaugggacu ucggaagcgu gggcggcguc     1860

uucaacagcc ucggaaaagg uauccaccaa aucuucgggg cagccuucaa aagccucuuc     1920

gggggaauga gcugguucuc acaaauacuu auagggacac ugcuaguaug gcucggccuc     1980

aacaccaaaa acggaagcau aagccuaacc ugccuagcac ugggaggcgu gaugauauuc     2040

uuaagcacag ccgucucggc a                                               2061


<210>  2964
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short CDS opt

<400>  2964
acgagcauag gaaucgucgg acuacuacua accaccgcaa uggccgcaga aaucacacgc       60

agaggaagcg cauacuacau guaccuagau aggagcgaug ccggcaaagc caucagcuuc      120

gcgacaacac uaggugucaa caaaugccac guacaaauaa uggaccuagg ccauaugugc      180

gacgccacca ugucauacga augccccaug cuagacgaag gcgucgaacc agacgacgug      240

gacugcuggu guaauaccac auccaccugg guagucuacg gcacaugcca gcacaagaaa      300

ggagaggcac gacgcucaag acgagcggua acacuaccca gccacucaac ccgcaaacug      360

caaacgcgcu cacaaacaug gcuagaauca agggaauaca caaaacaccu aauaaaaguc      420

gaaaacugga uauuccgcaa ccccggcuuc gcacuggucg caguagcaau agcguggcuc      480

cuaggcucga guaccuccca aaaagucaua uaccucguca ugauccuccu cauagcccca      540

gcauacucca uccgcugcau cggcgucagc aacagagacu ucguagaagg caugucugga      600

ggcacauggg uggacguggu gcuagaacac ggaggcugcg ucaccguaau ggcccaagac      660

aaacccacag ucgacaucga acucgucaca accacgguua gcaacauggc agagguacgc      720

uccuacugcu augaagccag cauaucagac auggcaagcg acucgcgcug ccccacccaa      780

ggcgaagccu accucgacaa acaaagugac acgcaauacg uaugcaaaag aacccuaguc      840

gaccgaggcu ggggaaacgg uugcggccuc uucggaaaag gcagccuagu aacaugcgcc      900

aaauucacau gcuccaagaa aaugacaggg aaaagcauac aacccgaaaa ccuggaauac      960

agaaucaugc uuagcguaca cggcagccaa cauucaggua ugauaguaaa cgacaucggc     1020

cacgaaaccg augagaaccg cgccaaagua gaagucaccc ccaauucccc gcgcgccgaa     1080

gcaacccuag gcggauucgg aucacucggc cuugacugcg aaccccgcac aggacucgac     1140

uucagcgacc ucuacuaccu aacaaugaac aacaaacacu ggcucguaca caaagagugg     1200

uuucacgaca ucccccuacc cuggcacgcc ggagccgaca ccggaacacc ccacuggaac     1260

aacaaagaag cccuagugga guucaaagac gcacacgcca aacgccaaac cguagucguc     1320

cuaggcagcc aagaaggcgc cguccacacu gcguuagcag gagcauugga agccgaaaug     1380

gacggggcca aaggucggcu auucagcggc caccugaaau gccgccucaa aauggacaaa     1440

cuucgccuaa aaggcgucuc cuauucacuc ugcaccgccg uaugcacagc agccaaagua     1500

ccggcggaaa cccuacacgg caccguaacg gucgaaguac aauacgcagg aacagacggu     1560

ccaugcaaag uacccgcuca aauggccgua gacaugcaaa cccucacccc cguaggcaga     1620

cuaaucaccg ccaacccagu aauaaccgaa agcacggaaa acuccaaaau gaugcuagaa     1680

cuagacccgc ccuucggcga cagcuacauc gucauuggag uaggggacaa gaaaaucaca     1740

caccacuggc acagauccgg auccacaaua ggcaaagcgu ucgaagcaac cguccgcggc     1800

gcaaaacgca uggccguccu cggcgacacg gcgugggacu ucgguucagu aggcggaguc     1860

uucaacuccc uaggcaaagg aauacaccaa aucuucggcg ccgcauucaa aucgcuguuc     1920

ggcggcaugu cgugguucuc acaaauacuc aucgggaccc uccuaguaug gcugggacuc     1980

aauaccaaaa acggaucaau cucacucaca ugccuggcgc ugggcggcgu aaugauauuc     2040

cuaucaacag cagucucggc c                                               2061


<210>  2965
<211>  2043
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short CDS opt

<400>  2965
acuagcaucg gcaucgucgg ccuccuacug accacagcua uggcagcaga aaucacccga       60

cggggguccg cauacuacau guaccuagac cgcucagacg ccggaaaagc gaucuccuuc      120

gcaacaacgc uaggagucaa caaaugccac guccaaauca uggaccuagg ccacaugugc      180

gacgcuacaa ugagcuacga augccccaug cuagaugagg gcguagaacc cgacgacguc      240

gacugcuggu gcaacaccac uagcacgugg guaguauaug gcacaugcca ccacaaaaag      300

ggagaagcac ggcggagccg acgcgcagua acacucccaa gccacagcac acgaaaacuc      360

caaacccgua gccaaacgug gcucgaaucc agagaauaca ccaaacaccu aaucaaagua      420

gaaaacugga ucuuccgaaa cccaggcuuc gcccucguag cagucgcaau agcauggcuc      480

cuaggaucca guacaagcca gaaaguaauc uaccuaguaa ugauccuccu aauagcaccc      540

gccuacucca ucagaugcau aggcguaagc aaccgagacu uuguggaagg aauguccggc      600

ggaacauggg uagacgucgu gcuggaacac ggaggcugcg uaacgguaau ggcgcaagac      660

aaacccaccg uagacaucga acuggucacc accaccguau caaacauggc agaaguacgc      720

uccuacugcu acgaagcguc aauaucagac auggccucgg acucccggug cccaacccaa      780

ggcgaggcau accuagacaa acaauccgac acccaauacg ucugcaaacg aacccugguu      840

gaucgaggau gggguaacgg cugcggacuc uucgguaaag gcagucuagu caccugugca      900

aaauucacau gcucaaagaa aaugaccgga aaaucaauuc aaccagaaaa ccucgaauau      960

cgaauaaugc ucucaguaca cggaagccaa cacucaggca ugauagucaa ugacgaaaac     1020

cgcgccaaag uugaagucac cccaaacagu cccagagcag aagccacgcu cggcggguuc     1080

gggagccuag gacuggacug ugaaccccgg acgggccugg acuucagcga ccuauacuac     1140

cucaccauga acaacaaaca cuggcuagua cacaaagaau gguuucacga uauaccccuc     1200

ccguggcacg ccggcgccga uaccggcacu ccccacugga acaacaaaga agcacugguc     1260

gaguucaaag acgcacacgc aaaacgccaa acugucguag uccucggguc ccaagaaggc     1320

gccguacaca cagcacucgc cggcgcgcuc gaggcagaaa uggacggcgc caaagguaga     1380

cucuucucag gacaccuaaa gugccgccuu aaaauggaca aacuccgccu caaaggagug     1440

ucguacuccu uaugcaccgc cgcauucacc uucacaaaag uaccagccga gacacuccac     1500

ggcaccguaa ccguagaagu acaauacgcc gggaccgacg gaccaugcaa gguacccgca     1560

caaauggccg uagacaugca aacacucacg ccagucggac gccucaucac cgcaaauccc     1620

gucaucacag aguccaccga gaacuccaaa augaugcucg aacucgaucc cccauucggc     1680

gacuccuaca uagucaucgg cgucggagac aagaaaauca cccaccacug gcaccgaucc     1740

ggcucgacca ucggcaaagc cuucgaagcc accguaagag gcgccaaacg gauggccguc     1800

cugggcgaca cggccuggga cuucggaucc gucgguggcg uuuucaacuc acucggaaaa     1860

ggaauccacc aaaucuucgg agccgccuuc aaaucacuau ucggaggcau guccugguuc     1920

agccaaaucc ucaucggaac ccuacucguc uggcuugguc ucaacaccaa aaacggcagc     1980

aucagcuuaa caugccucgc acucggaggc guaaugaucu uucucuccac cgccgucagc     2040

gca                                                                   2043


<210>  2966
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short CDS opt

<400>  2966
acaagcauug gcauuauagg ccuacuucuc accacugcca uggccgccga aauaacacga       60

cgaggaagcg ccuacuacau guaccuagac agaucggacg caggcaaagc aaucagcuuc      120

gcaacaaccc ucggagucaa caaaugccac guacaaauca uggaccuagg acauaugugc      180

gacgcaacaa ugucauacga augccccaug cuggacgaag gcgucgaacc cgacgacgua      240

gacugcuggu gcaacaccac aaguaccugg guagucuacg gaaccugcca ccacaaaaag      300

ggagaaaccc gacggucccg cagaucagua ucccuccgcu accacuacac aagaaaacuc      360

caaacccgaa gccaaaccug gcucgaaucc cgcgaauaca aaaaacaccu aaucauggua      420

gaaaacugga uauuccggaa cccaggauuc gccaucguga guguagccau cacauggcuc      480

augggcaguc ugaccagcca aaaagucauc uaccucguaa ugaucgugcu aauaguaccc      540

gccuacagca ucucaugcau cggcgucuca aaccgcgacc ucguagaagg aauguccgga      600

ggcaccuggg ucgacguggu ucucgaacau ggaggaugcg ugacggaaau ggcccaagac      660

aaaccaaccg ucgacaucga acucguaacg augaccguca gcaacauggc agaagucaga      720

uccuacugcu augaagcguc ccucagcgac auggcaagcg ccagccgaug ccccacacag      780

ggcgagcccu cacucgacaa acaaucagac acacaaagcg ucuguaaaag aacccucggg      840

gaccgaggcu ggggcaacgg gugcggcaua uucggaaaag guucgcuagu aacaugcuca      900

aaauucaccu gcugcaaaaa aaugcccggc aaaucgauac aaccagagaa ccucgaauac      960

agaauaaugc uacccguaca cgggagccaa cacagcggca ugauaguaaa cgacauagga     1020

cacgagacag acgagaacag agcaaaagua gaagucacgc caaauucccc gcgagccgaa     1080

gcaacacucg gaggcuucgg aagccuaggg cucgacugcg aaccacgcac cggccucgac     1140

uucucagacc uauacuaccu gaccaugaac aacaaacacu ggcuugucca caaagaaugg     1200

uuccacgaua ucccucuacc cuggcacgcc ggagcagaca ccggcacacc ccacuggaac     1260

aacaaagagg cccugguaga guucaaagac gcacacgcaa aacgucaaac cgucgugguc     1320

uuaggcagcc aagaaggcgc cguacacacc gcacucgccg gcgcccucga agcggagaug     1380

gacggggcua aaggacgccu guucucaggc caucucaaau gccgucuaaa aauggacaaa     1440

cuaagacuaa aaggaguauc guacagccuc ugcaccgccg ccuucacauu caccaaagua     1500

ccggcagaaa cacugcacgg aacagugaca guggaagucc aaagcgccgg aacagaugga     1560

cccugcaaag uccccgccca gauggccgua gacaugcaaa cacucacacc cguaggccgg     1620

cuaaucacag caaacccggu aaucaccgag ucaacagaaa acuccaaaau gaugcuggaa     1680

cuggauccgc cauucggaga cucauacaua gucauaggug uaggagacaa gaaaauaacc     1740

caccacuggc accgaagcgg cagcaccaua ggaaaagccu ucgaagccac gguacgcgga     1800

gccaaaagaa uggccgugcu cggagacacc gccugggacu ucggcucagu agggggcgua     1860

uucaacagcc uaggaaaagg cauacaccaa aucuucggag cagcauuuaa aagccuauuc     1920

ggaggaauga gcugguucuc acaaauacua aucggaaccc uacuagugug gcuaggacuc     1980

aacaccaaaa acggcagcau cucacucacg ugccuagccc ugggaggagu caugauauuc     2040

cucuccacag ccguauccgc u                                               2061


<210>  2967
<211>  2058
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short CDS opt

<400>  2967
agcguaggca uagucggccu acuccugacg accgcaaugg ccgcagaagu gacacggcgg       60

ggcagcgccu acuacaugua ucuggacaga aacgacgcgg gggaagccau cucauuccca      120

acgacccuag gcaugaacaa augcuacaua caaauaaugg accuagggca caugugcgac      180

gcaacgaugu ccuacgagug cccgaugcuc gacgaaggug uagaaccaga cgacgucgau      240

ugcuggugca acaccacauc cacaugggua guauacggca cuugccacca uaagaaaggg      300

gaagcacgcc gcagccgacg cgccguaaca cucccuagcc acuccacgcg caaacuacaa      360

acaagaagcc aaaccuggcu agaaucuaga gaauacacga aacaccugau aagaguagaa      420

aacuggaucu uccgaaaccc gggauucgcc cucgccgcag ccgccauagc auggcuccuc      480

ggaagcagua ccagucaaaa aguaaucuac cugguaauga uccuccuaau cgcacccgcg      540

uacagcauca ggugcaucgg cgugagcaac cgcgacuucg uagaagggau gagcggcgga      600

acaugggucg acgucguccu agagcacgga ggaugcguca caguaauggc acaagacaaa      660

ccuacgguag acaucgagcu cgucaccacc accguaagua acauggccga aguacgguca      720

uauugcuacg aagcauccau aagcgacaug gccagcgacu cccgaugucc aacucaagga      780

gaagcauacc uagacaaaca aucggacaca caauacguau gcaaaagaac ccucguagac      840

agaggauggg gcaacggaug cggacuauuc ggaaaaggcu cgcuaguaac cugcgccaaa      900

uucgcgugcu ccaagaaaau gacaggaaaa agcauucaac cagaaaaccu agaauacaga      960

auaaugcugu ccguacacgg aagccaacau agcggaauga ucgucaacga caccggacac     1020

gaaacggacg agaacagagc aaaaguagaa aucaccccaa acaguccccg cgcagaggcg     1080

acgcuaggag guuucggaag ccuaggacug gacugcgaac cccgcacggg ccucgacuuc     1140

agcgaccugu acuaccucac caugaacaac aaacacuggc uagugcacaa agaaugguuu     1200

cacgacaucc cccuaccgug gcacgcaggc gcagacacag gaacaccgca cuggaacaac     1260

aaagaagcac uggucgaguu caaagacgcc cacgcaaaaa gacagacagu ggugguccug     1320

ggcagccaag agggagccgu acacacagcg cucgcgggag cacuggaagc cgagauggac     1380

ggcgcaaaag gacgccuguc cucaggacac cucaaaugcc gacuaaaaau ggacaaacuc     1440

agacucaaag gagucagcua cucacuuugu acagccgccu ucacauucac aaaaauacca     1500

gccgaaacac uacacggaac aguaaccguc gaaguacagu acgcaggaac agacggaccg     1560

ugcaaagucc ccgcgcaaau ggcaguagac augcaaaccc ugaccccagu cggacgacua     1620

auaaccgcaa accccguaau cacagaaucc accgaaaauu ccaaaaugau gcucgagcua     1680

gacccacccu ucggcgacag cuacauagug aucggcgucg gggaaaagaa aauaacccac     1740

cacuggcacc gaagugggag uacaauaggc aaagcauucg aggcaaccgu cagaggggcc     1800

aaacgaaugg caguacuggg agacacagca ugggacuucg gaucaguggg cggagcccua     1860

aacucacucg gaaaaggaau ccaucaaauc uucggggccg cguucaaauc acucuucggu     1920

ggcaugagcu gguucagcca aauacucaua ggcacacucc uaauguggcu cggacugaac     1980

acgaaaaacg gaagcauaag ccugaugugc cuggcacuag gcggcguccu cauauuuuua     2040

aguacagccg uaagcgcc                                                   2058


<210>  2968
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short CDS opt

<400>  2968
accuccgugg ggauagucgg acuacuccug acaaccgcca uggccgccga aguuacccga       60

cgaggcagcg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc aaucuccuuc      120

cccaccacgc uaggcaugaa caaaugcuac auacaaauca uggaccucgg acacaugugc      180

gacgcaacaa ugagcuacga augccccaug cucgacgagg gagucgaacc cgacgauguc      240

gacugcuggu gcaacacaac cagcaccugg gucguguacg gaaccugcca ccacaaaaag      300

ggcgaagcgc gccgcucccg gcgcgccguc acccucccca gccacucaac acgaaaacua      360

caaacacgaa gccaaacaug gcuggaauca cgagaauaca ccaaacaccu aauacgaguc      420

gaaaacugga uauuccgaaa ccccggcuuc gcacuagccg ccgcggccau agccuggcuc      480

cucggcucau caaccuccca aaaaguaauc uaccucguaa ugauacuccu aauagccccc      540

gcauacucga uaagaugcau aggcgucagc aaccgcgacu ucguagaagg aaugagcggc      600

ggcaccuggg ucgacguugu ccuagaacac ggcggaugcg ucacaguaau ggcccaagac      660

aaaccuaccg ucgacauaga acucgucaca acaaccguuu ccaacauggc cgaaguacga      720

ucauacugcu acgaggcuuc aauauccgac auggccucgg acucccgcug ccccacccaa      780

ggcgaagccu accucgacaa acagagcgac acccaauacg ucugcaaacg cacgcuaguc      840

gaccgcggau ggggcaacgg cugcggacua uucgggaaag gaucccuagu aacaugugca      900

aaauucgccu gcucaaagaa aaugacgggu aaaucaaucc aaccggaaaa ccucgaguau      960

cgaaucaugc ucuccgucca uggcucccaa cacuccggca ugauaguaaa ugacacaggc     1020

cacgaaaccg augaaaacag agcuaaaguu gaaauaaccc ccaacucacc acgcgccgaa     1080

gccacccugg gcggcuuugg aucacugggc cucgacugcg aaccccgaac cggucucgac     1140

uucagcgacc uguacuaccu aacaaugaac aacaaacacu ggcuagucca caaggagugg     1200

uuccacgaca ucccacuccc auggcacgcc ggagcagaca caggcacccc acacuggaac     1260

aacaaggaag cccuagucga guucaaagac gcacacgcaa aacgacaaac ggucgucguc     1320

cuagggagcc aagaaggagc aguacacaca gcacucgcag gggcgcuaga agcagaaaug     1380

gacggggcaa aaggacgccu uuccaguggg cacuuaaagu gcagacucaa aauggacaaa     1440

cuuagauuaa aaggcguauc auacucacua ugcaccgccg ccuucaccuu caccaaaaua     1500

cccgccgaaa cgcugcacgg aacagucacg guugaagucc aauacgccgg cacagacggg     1560

cccugcaaag uacccgcgca aauggcgguc gacaugcaga cacuuacccc cguuggucgg     1620

cuaauaacgg ccaacccagu cauaacagaa agcaccgaaa acuccaaaau gaugcuagaa     1680

cucgacccac ccuucggaga cagcuacauc gucaucggcg uuggcgaaaa gaaaaucaca     1740

caucacuggc acagaucagg uagcaccaua ggcaaagccu uugaagccac agucagaggc     1800

gccaggcgua uggcaguccu cggagacacc gccugggacu uuggauccgu gggaggcgca     1860

cuaaacuccc uaggcaaagg cauccaucaa auauucgggg ccgccuucaa aucgcuguuc     1920

ggcggaaugu caugguucag ccaaauccua aucggaacgc uccuuaugug gcucggccua     1980

aacaccaaaa acggauccau aucccucaug ugccuggccc ucggaggcgu ccuaaucuuc     2040

cuguccacag cggucuccgc g                                               2061


<210>  2969
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short CDS opt

<400>  2969
accuccguug gcaucguggg ccuccuccua acaaccgcaa uggccgccga aguaacccga       60

cguggaagug cauacuacau guaccuagac cgcaacgacg caggagaagc aaucucauuc      120

ccaaccaccc uaggcaugaa caaaugcuac auccaaauca uggaccuagg ccacaugugc      180

gacgcaacca ugucauacga augccccaug cuagacgaag guguugaacc agacgacgua      240

gacugcuggu gcaacacaac auccaccugg guagucuacg gcacaugcca ccacaaaaag      300

ggagaagcac ggcgcucccg ccgagccguc acccugccca gucacuccac ccgcaaacuc      360

caaacccgau cccaaaccug gcuagaaagc cgcgaauaca caaaacaccu aauaagagua      420

gaaaacugga uguucagaaa ccccggauuc gcccucgccg cagcagccau agcauggcuc      480

cucggcucau ccaccucaca aaaaguuaua uaccucguca ugauccuacu aauagcccca      540

gccuauagca ucagaugcau cggcgucucc aaccgagacu ucgucgaagg aaugucagga      600

ggaaccuggg uggacgucgu gcucgaacac gggggcugcg ugaccguaau ggcacaagac      660

aaaccaacgg uagacaucga gcuagucaca acaacugugu ccaacauggc cgaaguacga      720

agcuauugcu acgaagcauc caucucggac auggccagcg acucucgaug ccccacccaa      780

ggcgaagcau accuggacaa acaauccgac acccaauacg uaugcaaacg uacucuagua      840

gaccgcggcu gggggaacgg uugcggacug uucggaaaag ggucacuggu aaccugcgca      900

aaguucgcgu gcucuaagaa aaugaccgga aaauccaucc aaccagaaaa ccuagaauac      960

cgcaucaugc uauccgucca cggaucccag cacaguggaa ugauugucaa cgacaccgga     1020

caugaaacag acgaaaaccg cgccaaagua gaaauaacac caaacucacc ccgcgccgaa     1080

gcaacacucg gcggauucgg cucacucgga cucgacugcg aaccccgcac cggccucgac     1140

uucucagacc uguacuaccu cacaaugaac aacaaacacu ggcucguaca caaagaaugg     1200

uuccacgaca uaccacuccc uuggcacgcg ggcgccgaca cagguacccc acacuggaac     1260

aacaaagaag cccuagucga guucaaagac gcacacgcca aaagacagac gguagucgug     1320

cucggaagcc aggaaggagc cguacacacc gcccucgccg gggcacugga agcagaaaug     1380

gacggcgcua aaggccgccu cucauccggc caccuaaaau gccgguuaaa aauggacaaa     1440

cugagacuca aaggaguaag cuacuccuua ugcaccgccg ccuucaccuu caccaaaaua     1500

ccggccgaaa cccuccacgg uaccguaaca guagaagucc aauacgccgg aacagacggc     1560

cccugcaaag ucccugccca gauggcuguc gacaugcaaa cccucacccc cgucggaaga     1620

cuaaucacug ccaaccccgu cauaaccgaa uccaccgaaa acagcaaaau gauguuggaa     1680

cucgaucccc cauuuggcga cucauacauc gugauaggcg uaggcgagaa aaagauaaca     1740

caccacuggc accguuccgg aaguaccauc ggcaaagcau ucgaagcgac cguccgaggc     1800

gcaaaacgaa uggccguacu cggggacacg gccugggacu ucgggagcgu gggcggcgca     1860

cuaaacuccc ucggcaaagg aauccaccaa aucuucggcg ccgccuucaa gucccucuuc     1920

ggaggcaugu cgugguucuc ccagaucuua aucggaacuc uacucaugug gcuaggguug     1980

aacaccaaaa acggaucaau aucccuaaug ugcuuagccc uuggaggagu acuaaucuuc     2040

cuaagcaccg cagugucagc c                                               2061


<210>  2970
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short CDS opt

<400>  2970
accaguguag gcauaguggg acuacuacuc acuaccgcca uggcagccga aguaacaagg       60

cgaggauccg cguacuacau guacuuagac cgcaacgacg caggagaagc aaucuccuuu      120

cccaccacac ucggaaugaa caaaugcuac auccaaauaa uggaccuagg ccacaugugc      180

gacgccacaa uguccuacga augcccgaug cucgacgaag gagucgagcc agaugacgua      240

gacugcuggu gcaacaugac cagcaccugg gucgucuacg gcaccugcca ccacaaaaaa      300

ggagaggcgc gaagauccag acgggccguc acacuaccaa gucacuccac gcguaaacuc      360

cagaccagaa gccaaaccug gcucgaaucg cgagaauaca caaaacaccu aauccgcguc      420

gagaacugga uauuccguaa ccccggauuc gcccuagcag ccgccgcaau cgccuggcua      480

cuaggcagcu caaccagcca aaaaguuauc uaccuuguca ugauacuccu cauugcgcca      540

gcauacucga ucagauguau uggugucagu aaccgcgacu ucguagaagg cauguccggc      600

ggaacauggg ucgacgucgu ucucgaacac gggggaugcg ucaccgucau ggcacaagac      660

aaaccaaccg ucgacauaga acucguaacu acaaccgucu ccaacauggc cgaaguccgc      720

agcuacugcu acgaagccag caucucagac auggcgagcg auucccgcug ccccacgcaa      780

ggcgaagccu accuagacaa acaaagcgac acccaauacg uauguaaacg gacccucguc      840

gacagaggau ggggcaacgg cugcggccuu uucggcaaag gcagccuagu aaccugcgcc      900

aaauucgccu gcagcaaaaa aaugaccgga aaaagcauuc aaccugaaaa ccuagaguac      960

cgcaucaugc ucuccgucca cggaucgcaa cacuccggca ugauagucaa ugacaccgga     1020

cacgaaaccg acgaaaaccg ugcgaaagug gaaaucaccc cuaauagccc cagggccgaa     1080

gccacacucg gcggauucgg cagccuaggg cuagacugcg aacccagaac aggccuagac     1140

uucagcgacc uauacuaccu cacaaugaac aacaaacacu ggcuagucca caaagaaugg     1200

uuccacgaca ucccacuacc auggcacgcu ggagccgaca cagggacucc ccacuggaac     1260

aacaaagaag cgcucguaga guucaaagau gcacacgcaa aacggcaaac agucguaguc     1320

cuaggcucac aggaaggcgc gguccauaca gcccucgcag gagcacucga agcagaaaug     1380

gacggagcca aaggccgacu aagcagcggg caccucaaau gcagacucaa aauggacaaa     1440

cucagacuga aaggcguaag cuacagccuu ugcacagcag ccuucaccuu uaccaaaaua     1500

cccgccgaaa cccuacacgg caccguaacg guagaaguuc aauacgcagg caccgacgga     1560

ccaugcaagg uccccgcaca aauggccguc gauaugcaga cccuaacccc cgucggacgc     1620

cucaucaccg ccaacccagu cauaaccgaa agcacagaaa acucgaaaau gaugcucgaa     1680

cuagacccac ccuucggaga cucauacauc guaaucggag ucggagaaaa aaaaauaaca     1740

caucacuggc accgauccgg cucaacaaua ggcaaagccu ucgaagcaac aguacgagga     1800

gcgaaacgga uggccguacu cggagacaca gccugggacu ucggcagcgu cggaggagcc     1860

cucaacagcu uaggaaaagg cauccaccaa auauucggag ccgcauucaa gucccuguuc     1920

ggcggcaugu ccugguucag ccaaauacua auaggcacgc uacugaugug gcucggacuc     1980

aacaccaaaa acggcagcau cagccucaug ugccucgcgc ucggaggcgu ccucauauuc     2040

cuuucaaccg cagucucagc g                                               2061


<210>  2971
<211>  2061
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short CDS opt

<400>  2971
acaagcgucg ggaucguggg ccuccuacua acgaccgcaa uggcagcaga ggucacccgc       60

agaggaagcg cauacuacau guaccuagac cgaaacgacg ccggagaagc aaucagcuuc      120

cccacaaccc ucggcaugaa caaaugcuac auacagauca uggaccuagg acacaugugc      180

gacgccacca ugagcuacga augcccgaug cuagacgaag gcgucgaacc agacgacguc      240

gacugcuggu gcaacacaac aagcacaugg guagucuacg gaaccugcca ccauaagaaa      300

ggcgaagcgc gcagaagccg gcgagcagua acccuaccaa gccacuccac aagaaaacua      360

caaaccagau cacagaccug gcucgaaagc cgggaauaca ccaaacaccu cauccgcguc      420

gaaaacugga ucuucagaaa ucccggcuuc gcccuagcgg cagccgccau cgccuggcua      480

uuaggcagca guaccuccca gaaaguaaua uacuuaguca ugauacuacu aauagcgcca      540

gcguacucga uacgaugcau aggagucagc aaccgagacu ucgucgaagg caugagcgga      600

gggacguggg uggacguagu acucgaacac ggaggcugcg ucacggcgau ggcucaagac      660

aaacccacag ucgacaucga gcuagucacc acaaccguaa gcaacauggc cgagguaaga      720

ucauacugcu acgaggcauc cauaagcgac auggcaagug acucaagaug ccccacacaa      780

ggagaagcau accucgacaa acaguccgac acccaauacg ucuguaaaag aacccuagua      840

gacagaggcu ggggcaacgg gugcggacua uucggcaaag gcagccucgu gacaugcgcg      900

aaauucgcuu gcuccaagaa aaugacaggc aaauccaucc aacccgaaaa ccucgaauac      960

cgcauaaugc uaagcguaca cgggucccaa cacuccggca ugcuggugaa cgauaccgga     1020

cacgagaccg acgaaaacag agcgaaagua gaaauaaccc caaauagccc gcgagcagaa     1080

gcaacucuag gcggauucgg cagccuugga cuagacugcg aaccaagaac aggccucgac     1140

uucucagacc uauacuaccu caccaugaac aacaaacacu ggcucgcgca caaagaaugg     1200

uuccacgaua ucccccuccc cuggcacgca ggcgcagcaa cgggaacacc acacuggaac     1260

aacaaagaag cccucgucga guucaaagac gcgcacgcca aaaggcaaac cgucguagua     1320

cuaggauccc aagaaggagc cguccacacc gcacucgccg gagcacuaga agccgagaug     1380

gacggcgcaa aaggacgacu cuccagcggc caccucaaau gccgacucaa aauggacaaa     1440

cugcgccuua aaggcgucuc guacuccuua ugcacggccg ccuucaccuu uacaaaaaua     1500

cccgcagaga ccguagacgg caccgucacc guagaagggc aguacggggg aaccgaugga     1560

ccuugcaaag uaccagccca aauggcagua gacaugcaga cccuuacacc cguaggacga     1620

cuaauaacag ccaaccccgu cauaaccgaa uccaccgaaa acagcaaaau gaugcuagaa     1680

cuagaucccc cauucggcga cucauacauc gugaucgggg ucggagaaaa gaaaauaacc     1740

caccacuggc acaggagcgg gagcacaauc ggcaaagccu ucgaagccac cguacggggc     1800

gccaaacgaa uggccguucu cggcgacaca gcaugggacu ucggcucggu cggcggagca     1860

cuaaacagcc ucggaaaagg aauacaccaa auaauaggcg cggccuucaa aucacuauuc     1920

gggggcauga gcugguucag ccaaauacug auagggacgc ugcugaugug gcucggacug     1980

aauacgaaaa acggcuccau cagucuaaug ugccuagccc uaggaggcgu acugauauuc     2040

cucagcacag caguauccgg c                                               2061


<210>  2972
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short CDS opt

<400>  2972
ggcgccgacg ccagcaucgg caucgucucc cuacuccuaa caaccguaau ggcagcagag       60

auaacgcgac gaggauccgc guacuacaug uaccuagaca gaagcgacgc cggcaaggcc      120

aucucauucg ucacaacgcu aggcgucaac aaaugccacg uucaaauaau ggaucucggc      180

cacaugugug acgcuaccau gagcuacgaa ugcccaaugc ucgacgaggg cgucgaaccc      240

gacgacgugg acugcuggug caacaccacc ucaacauggg ucguauaugg cacgugccac      300

cacaagaaag gcgaagcccg ccgaucaagg agagccguca cccucccaag ccacucaacc      360

agaaaacucc aaaccagaag ccagaccugg cucgagagcc gagaauacac aaaacaccug      420

auaaaaguag aaaacuggau cuuccgaaac ccaggcuucg cgcuagucgc aguagccaua      480

gccuggcucc ucggcagcuc cacaucacag aagguuauau accuagucau gauacuccuc      540

aucgccccag cauacagcau ccgcugcaua ggcguaagca accgcgacuu cgucgagggg      600

auguccggag gaacgugggu cgacguagug cuagaacacg guggcugcgu aaccgucaug      660

gcgcaagaca aaccgaccgu cgacaucgag cuagucacaa cgacaguauc aaacauggcc      720

gaaguacgaa gcuacugcua cgaagccagc aucagcgaca uggccucaga cagcagaugu      780

ccaacccaag gagaagcaua ccucgacaaa caaagcgaca cgcaauacgu cugcaaacgc      840

acccuaguag accguggaug gggcaacggc ugcggccugu uuggaaaagg cucacugguc      900

acaugugcca aauuuacaug cagcaagaaa augacgggca aaagcauaca accagaaaac      960

cuagaauacc gcaucaugcu aagcguccac ggaagccaac acuccggaau gauagucaac     1020

gacgaaaacc gagcaaaagu cgaaguaaca cccaacucac caagagcaga agcaacacuc     1080

ggcggcuucg gaagccuggg ccuagacugc gaacccagaa ccggacuaga cuucagcgac     1140

cuguacuacc ucaccaugaa caacaaacac uggcucgucc acaaggaaug guuccacgac     1200

auaccacuac cauggcacuc aggagccgac accgaaaccc cacacuggaa caacaaagaa     1260

gcacuggugg aauuuaaaga cgcacacgcc aaaagacaga ccgucguagu ccucgggagc     1320

caagaaggcg ccguccauac agcacuggcc ggagcccuag aggccgaaau ggacggagca     1380

aaaggacgcc uaucauccgg ccaccucaaa ugccgccuca aaauggacaa acuccgacua     1440

aaaggcguau ccuacucccu gugcaccgcc gcauucacau ucacaaaagu accagccgaa     1500

acacugcacg gcacggugac cguagaaguc caauacgcag gacgcgacgg cccaugcaag     1560

guccccgcac aaauggcagu agacaugcag acauuaacgc cagucggaag acucauaacc     1620

gccaaccccg ucauaacuga aagcaccgaa aacucaaaaa ugaugcuuga acuagaccca     1680

cccuucggag acuccuauau cgucauagga guaggcgaca agaaaauaac acaccacugg     1740

cacagaucag gcagcauaau cgggaaagcc uucgaggcca caguccgcgg cgccaaacgc     1800

auggcagucc uaggggacac agcaugggac uucggcuccg ugggcggagu uuucaacucc     1860

cuaggaaagg gaauccacca aaucuucggc gccgccuuca aaucacucuu cggaggaaug     1920

uccugguucu cucaaauccu aaucggaacc cuacucgucu ggcucggccu aaacaccaag     1980

aacggcagca uaagccuaac cugccucgcg cucggaggcg uaaugauauu ccucucaacu     2040

gcggucagcg ca                                                         2052


<210>  2973
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short CDS opt

<400>  2973
ggagcagaca ccagcaucgg aaucauagga cuacuccuaa caacagcuau ggcggccgaa       60

aucacgagaa gaggaagcgc auacuauaug uaccucgacc guuccgacgc aggcaaagcg      120

auaucauucg caaccacaau gggcgugaac aaaugccacg uccaaaucau ggaccuaggu      180

cacaugugug acgccaccau gucuuacgaa ugccccaugc ucgacgaagg agucgaaccc      240

gacgacguag acugcuggug caacaccacc uccacauggg uuguguacgg aacaugccau      300

cacaagaaag gagaagcccg gccgucccgc cgcgcaguaa cgcucccgag ccacuccacc      360

agaaaacuac aaacccgcuc acaaacaugg cucgaaaguc gcgaguacac aaaacaucuc      420

auaaaagucg aaaacuggau acuacgcaac ccaggcuuca caguggccgc gcucgccaug      480

gccuggcuac ugggaaacag uacgagccag cgcguaaucu accucguaau gauucuccug      540

aucgcacccg cauacuccau acgcugcauc ggcguuagca accgcgacuu cguagaagga      600

auguccggag gaaccugggu agacgugguc cuagaacacg gaggaugcgu caccgucaug      660

gcccaagaca aaccaacagu cgacauagag cuaguaacaa ccacggucuc caacauggcc      720

gaagucagaa gcuacugcua cgaagcaucc aucagcgaca uggcaagcga cucccgcugu      780

cccacacaag gggaagccua ccucgacaaa caguccgaca cacaauacgu cugcaaacga      840

acccucgugg aucgcggaug gggcaacgga ugcggacucu ucggaaaagg aagccuagug      900

accugugcaa aguucacgug cuccaagaaa augacaggaa aauccauaca acccgaaaac      960

cucgaauacc ggaucaugcu cagcguccac ggaucccagc acagcggaau gauagugaac     1020

gacauaggcc acgaaaccga cgaaaaccga gcaaaagugg aagucacccc uaauucaccc     1080

cgcgcagaag caacacuggg cggcuucggc agccucggcc uagacugcga accgcgcacc     1140

ggacuagacu uuucagaccu guacuaucua acaaugaaca acaaacacug gcuuguccau     1200

aaagaauggu uccacgacau cccccuccca uggcaugcag gcgcagacac aggcacaccc     1260

cacuggaaca acaaagaggc acucguggag uucaaagacg cacacgccaa aagacaaacc     1320

gucguaguac ucggcuccca agaaggagca guccacaccg cucuggcagg agcauuggag     1380

gcagaaaugg acggcgcgaa gggccgacuc uucucaggac accucaaaug ccggauaaaa     1440

auggacaaac uaaggcuaaa aggcgucagc uacucacucu gcaccgccgc auucacauuc     1500

agcaaagucc cggcagaaac gcuccacgga acagucacgg uggaggugca auacgccggc     1560

cgagacggac ccugcaaagu ccccgcccaa auggcaguag acaugcaaac gcucaccccc     1620

guaggaagac uuaucacggc caacccaaua auaacagaau ccaccgaaaa cagcagaaug     1680

augcucgagc ucgacccucc cuucggcgac ucauacauag uuaucggagu aggcgacaag     1740

aaaaucacgc accacuggca caggucaggc agcacaaucg gaaaagccuu cgaagccacc     1800

guccgcggcg caaaaagaau ggccguacuc ggcgacacag ccugggacuu uggaagcguc     1860

ggcggagucu ucaacucacu agggaaagga guucaccaag uauucggagc cgccuucaaa     1920

ucgcuauucg gcgggauguc cugguucucg caaauccuca ucggaacccu ucuagucugg     1980

cucggacuga acaccaaaaa cggaagcauc ucccuagcau gccuagcccu cggcggugca     2040

augauauucc uaagcacggc cguaagcgcc                                      2070


<210>  2974
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE CDS opt

<400>  2974
ucgcaaaagg ucauauaccu ggucaugauc uuacucauag ccccggcaua cagcauaaga       60

uguauaggag ucucgaacag agacuucguc gaaggaauga gcggaggcac guggguggac      120

auaguccugg aacacggagg augcgugacc gugauggcgc aagacaaacc gaccguggac      180

auagaacugg uaacaaccac ggucucgaac auggccgagg ucaggagcua cugcuacgaa      240

gcaagcauaa gcgacauggc aagcgauagu agaugcccga cacaaggcga ggcauaucua      300

gacaaacaaa gcgauaccca auacguaugu aagcgcacau uaguggaccg aggaugggga      360

aacggcugcg gacuguucgg gaaaggaagc cuagucacgu gugcgaaguu cgcgugcagc      420

aagaaaauga ccggcaaauc gauacaaccc gagaaccuag aauacagaau aaugcuaagc      480

guacacggga gccagcacuc gggcaugauc guaaacgaca ccggacacga gaccgacgaa      540

aaccgagcga aaguggaaau aacacccaac ucgccccgag cagaggccac ccuaggagga      600

uucggcucgc ucgggcuaga cugcgagccc aggacugguc uagacuucuc agacuuguac      660

uaccucacga ugaacaacaa gcacuggcua guacacaagg aaugguucca cgacauacca      720

cugcccuggc acgcgggagc cgacacaggu acaccccacu ggaauaacaa ggaggcccuc      780

guggaguuca aagacgccca cgcaaaacgc caaaccgugg uaguacucgg cagccaggaa      840

ggggccgucc acacggcccu ggcaggggca cuggaagcgg aaauggacgg ggcaaagggc      900

agacuaucga gcggacaccu aaaaugucgc cuaaaaaugg acaagcuaag gcucaagggc      960

guaaguuaca gccuaugcac ggccgcauuc acguucacga aaauacccgc agaaacgcua     1020

cacggcacgg ucacggucga gguccaauac gcgggcacag acggacccug caaagugccc     1080

gcgcaaaugg ccguggacau gcaaacgcuc acgcccgucg gacggcucau aaccgcaaac     1140

ccgguaauaa ccgaaaguac ggaaaacagc aaaaugaugc ucgaguuaga cccaccauuc     1200

ggggacucau acauaguaau aggcgugggc gagaaaaaga uaacgcacca cuggcaccgc     1260

ucgggaucua ccaucggcaa ggcuuuugaa gcaacaguac gcggggcaaa acgcauggcc     1320

gugcucgggg acacggccug ggacuuuggc ucg                                  1353


<210>  2975
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE CDS opt

<400>  2975
ucgcaaaaag ucaucuaccu agucaugauc cuacucauag cccccgccua cagcauccgc       60

ugcaucggag ucucaaaccg cgacuucguu gaaggcaugu caggaggcac augggucgac      120

gugguacuag aacacggcgg gugcgucaca gucauggccc aagacaaacc cacaguggac      180

aucgaacugg ucaccaccac aguaucaaac auggcagaag uuagaagcua cugcuacgag      240

gcaagcauau ccgacauggc cagcgacuca agaugcccaa cacaaggcga agccuaccug      300

gacaaacaaa gcgacacuca auacguaugc aaacgaacgc uaguggacag aggaugggga      360

aacggaugug gccucuucgg caaaggcagc cucgucacau gcgcaaaauu cgccugcuca      420

aagaaaauga ccggaaaauc cauucaaccc gagaaccuag aauacagaau caugcuaucc      480

gugcacggca gccaacacuc cggaaugaua gucaacgaca ccggccacga aaccgacgaa      540

aaccgcgcaa aaguggaaau aaccccaaac ucacccagag ccgaagcaac acuaggcggc      600

uucggcuccc ucggacuaga cugcgaaccc agaaccggcc ucgacuucuc agaccuauac      660

uaucuaacca ugaacaacaa acacuggcuc gugcacaaag aaugguucca cgacauaccc      720

cuacccuggc acgcaggggc cgauacaggc acaccccacu ggaauaacaa agaagcccuc      780

guagaguuca aagaugcaca ugcaaaacga cagaccguag ucgugcucgg cagucaggaa      840

ggcgccgucc acacggcccu ggccggagcc cucgaagcag aaauggacgg cgcaaaaggc      900

cgacucucca gcggacaccu aaaaugccgc cuaaaaaugg acaaacuacg ccucaaaggc      960

gucagcuaca gccucugcac agcagcauuc acguucacaa aaauaccagc agaaacacuc     1020

cacggcacag uaacagucga aguccaguac gccggcacag acggacccug caaaguacca     1080

gcacaaaugg caguagacau gcaaacgcuc acgcccguag gacgccuaau caccgcaaau     1140

ccagucauca ccgaaagcac cgaaaacagc aaaaugaugc ucgaacucga ucccccauuc     1200

ggcgacucgu acauagucau cggcguggga gaaaagaaaa ucacacacca cuggcaccgc     1260

uccggaagca ccauaggcaa agccuucgaa gccaccguac gcggagccaa acgcauggca     1320

guacucggag acacagccug ggacuucggc ucc                                  1353


<210>  2976
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE CDS opt

<400>  2976
ucgcaaaaag uaauauaccu agucaugauc cuacucaucg ccccagcuua cagcauacgc       60

ugcaucgggg ucucaaacag ggacuucguc gaaggcauga gcggaggaac auggguggac      120

gugguacuag aacacggcgg augcguaacc guaauggcgc aagacaagcc uaccguagac      180

aucgaacuag uaaccaccac agucuccaac auggccgaag ucaggaguua cugcuacgaa      240

gcaagcauaa gcgacauggc gagcgacagc cggugcccga cacaagguga agcauaccua      300

gacaaacaaa gugacaccca auacgucugc aaaaggaccc uaguagacag gggcuggggc      360

aacggcugcg gucuauucgg aaaaggcagc cuagucacau gcgccaaauu caccugcagc      420

aaaaaaauga ccggcaaauc aauccaaccc gaaaaccucg aauaccgcau aaugcugagc      480

gugcacgggu cacaacacuc gggaaugauc ggauacgaaa cagacgaaga ccgcgcaaaa      540

guagaaguaa caccgaacuc gccaagagca gaagccaccc ucggaggauu cggcucucuc      600

ggacucgacu gugaaccaag aaccgggcua gacuucucag accucuacua ccuaacgaug      660

aacaacaaac acuggcucgu gcacaaagaa ugguuccacg acauaccccu gcccuggcac      720

gcaggcgcag acacgggcac cccacacugg aacaacaagg aagcacuagu agaauucaaa      780

gacgcccacg ccaagcguca aacaguaguc gugcucggga gccaagaagg ggccguacac      840

acggcccuag caggggcccu agaagcagaa auggacgggg caaaaggaag acuauucucc      900

ggacaccuga aaugccgccu aaaaauggac aaacucagac uaaagggcgu aagcuacagc      960

cuaugcacag ccgcauucac auucacaaaa guccccgcag aaacacuaca cggcaccguc     1020

accgucgaag uccaauacgc gggcacagac ggcccaugua aaauaccagu acaaauggca     1080

guagacaugc aaacacucac gccggugggc agacucauaa cagcaaaccc agucauaacc     1140

gaaagcacag aaaacaguaa aaugaugcuu gaacucgacc cacccuucgg ugacucguac     1200

aucgucauag gaguaggcga caaaaaaauc acgcaccacu ggcaccgauc agggucaacc     1260

auuggcaaag cauucgaagc aacaguacgc ggggccaaac gcauggccgu ccuaggggac     1320

accgccuggg acuucggauc a                                               1341


<210>  2977
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE CDS opt

<400>  2977
ucccaaaaag uaauauaccu aguaaugauc cuccucaucg caccggccua uaguauccgu       60

ugcauaggag ucagcaacag agacuuugua gaaggcauga gcggcggcac cugggucgac      120

gucguccugg aacacggagg augcgugacc guaauggcac aagacaaacc gaccguggac      180

aucgaacugg ucacaacaac agucucaaac auggccgaag ucagaagcua cugcuacgaa      240

gcaagcauaa gcgacauggc gagcgacagc cgaugcccaa cccagggaga agcauaccuc      300

gacaaacaga gcgacacaca auacguaugc aaacgaacgc ucguagacag gggcuggggc      360

aacggaugcg gccuauucgg aaaagggagc cuagucacgu gcgcgaaauu cgccugcagc      420

aagaaaauga caggaaaauc aauccagcca gaaaaccuag aguaccgaau caugcugagc      480

guacacggga gccaacacuc aggcaugauc guaaacgaca ccggacacga aaccgacgaa      540

aaccgcgcaa aaguagaaau aaccccaaac ucaccaaggg ccgaagcaac gcuaggcgga      600

uucggcucac ucggacucga cugcgagcca agaaccggac ucgacuucag ugaccuguac      660

uaccucacaa ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauacca      720

cuccccuggc acgcgggcgc agacacgggc acaccacacu ggaacaacaa agaagcccuc      780

guagaguuca aagacgcaca cgccaaacga cagaccgugg uaguacucgg aagccaggaa      840

ggggccgugc acaccgcccu ggccggcgcc cuggaagccg aaauggacgg agcaaaaggc      900

cgccucucaa gcgggcacuu aaaaugucgc cuaaaaaugg acaaacucag guuaaaaggc      960

guaagcuaca gucuaugcac ggccgcauuc acauucacca aaauaccagc ggaaacgcua     1020

cacggcacag ucacggucga aguccaauac gcgggcacag acgggcccug uaaaguacca     1080

gcacaaaugg ccguagacau gcaaacacua acaccggucg gccgacucau aaccgcgaac     1140

ccagucauaa ccgaaagcac agaaaacagu aagaugaugc uagaacuaga ucccccauuc     1200

ggagacagcu acauagucau aggcguaggu gagaaaaaga uaacacacca cuggcacaga     1260

agcggaucaa ccauaggaaa ggccuucgaa gcaaccguga gaggggcaaa aagaauggcc     1320

guacucggcg acaccgccug ggacuucggc ucg                                  1353


<210>  2978
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE CDS opt

<400>  2978
ucgcaaaaag uaaucuaccu agucaugauc uugcuaauag ccccggccua cagcauacgc       60

ugcaucggag uaucaaacag ggacuucguc gaaggaauga gcggaggcac guggguagac      120

gugguacuag aacacggcgg augcgucacc guaauggcgc aagacaaacc aaccguggac      180

aucgaacucg uaacgaccac gguaucaaac auggccgaag uaagaagcua cugcuacgaa      240

gcaagcauca gcgacauggc aagcgauagc cggugcccga cccaaggaga agcauaccua      300

gacaaacaga gcgacaccca auacguaugc aaacgaaccc uaguagacag gggcuggggc      360

aacggcugcg gacuauucgg aaaagggagc cuagucacau gcgccaaauu cacaugcagc      420

aagaaaauga ccgggaaauc aauccagccc gaaaaccuag aauaccgcau aaugcuaagc      480

gugcacggga gucagcacuc uggcaugauc gugaacgaca cgggauacga aacagacgaa      540

aacagagcaa aaguagaagu aacaccaaac uccccaagag ccgaagccac gcucggggga      600

uucggaucgc ucggacuaga cugcgaaccc cguaccgggc uagacuucuc agaccuauac      660

uaccuaacaa ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauacca      720

cugcccuggc acgcgggcgc cgacacugga acaccacacu ggaacaacaa agaagcccua      780

guagaguuca aagacgcaca ugcaaagaga cagaccgugg ucguacuggg aagccaagaa      840

ggagcaguac acacagcacu agcaggggca cuagaagcgg aaauggacgg ggccaaagga      900

aaacuauuca guggacacuu aaagugcaga cuaaaaaugg acaaacuaag acucaagggc      960

guaagcuaca gccuaugcac agcagcguuc acguucacga aagugccagc ggaaacgcua     1020

cacggaaccg ucacugucga aguccaauac gcgggcacag acgggcccug caaaaucccc     1080

guccaaaugg ccguagacau gcaaacgcua acacccgugg gaagacuaau aaccgcaaac     1140

ccagucauaa cagaaagcac ggaaaauagc aaaaugaugc ucgaacuaga cccaccguuc     1200

ggagacagcu acauaguaau aggcguagga gacaagaaaa ucacccacca cuggcaccgc     1260

ucgggcucaa ccauaggaaa agccuucgaa gcgaccguac gcggagccaa acgaauggcc     1320

guacuaggag acacggcaug ggacuucggc ucg                                  1353


<210>  2979
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE CDS opt

<400>  2979
ucacagaaag uaauauaccu aguaaugaua cuccucauag ccccggccua cuccauacgc       60

ugcaucggag ucagcaacag agacuucgua gagggaauga gcggcggaac auggguagac      120

gugguccuag aacacggcgg augcguaaca guaauggcac aagacaaacc aaccguggac      180

auagaauugg ucacaaccac aguaucgaac auggccgaag ucagaaguua cugcuacgaa      240

gcaagcauaa gcgacauggc aagcgacagc cgcugcccca cccaaggaga ggcauaccuc      300

gacaaacaaa gcgacacaca auacguaugc aaacgaacac ucguagaccg gggcuggggu      360

aacggcugcg gccuauucgg gaaagggagc cuagucacau gcgcgaaguu cacaugcagc      420

aagaaaauga ccggcaaaag cauccagccc gagaaccuag aauacagaau aaugcucagc      480

gugcacggga gccaacacuc gggcaugacc guaaacgaca ucggcuacga aacagacgaa      540

aaccgcgcca aaguagaagu aaccccgaac ucgccaagag ccgaagccac ccuaggugga      600

uucggauccc ucggacucga cugcgaacca agaaccggac ucgacuucuc ggaccuauac      660

uaccucacga ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauaccc      720

cuacccuggc acgccggagc agacacaggc acgccacacu ggaacaacaa agaagcgcua      780

guagaguuca aagacgccca cgccaaacga caaacagugg uaguguuagg aagccaagaa      840

ggggcagucc acacagcccu ggcaggagca cucgaagcag aaauggacgg agccaaaggc      900

aaacucuuua gcggacaccu caaaugccgc cuaaaaaugg acaaacuccg ccuaaagggc      960

guaaguuaca gccugugcac ggcagcguuc accuucacaa aagugcccgc ggaaacacuc     1020

cacggcaccg ucacggucga aguccaauac gcaggaacgg acggacccug caagauaccc     1080

guacaaaugg caguagauau gcagacucuc acccccgucg gaagacucau aaccgccaac     1140

ccgguaauaa cagaaagcac agaaaacagc aaaaugaugc ucgaacucga cccaccauuc     1200

ggggacucau acauagugau cggcgugggc gacaagaaaa ucacccacca cuggcaucgc     1260

ucgggcagca ccauaggcaa agccuucgaa gcaacagucc gcggggcaaa acgaauggcc     1320

guauuagggg acaccgcaug ggacuucggc ucg                                  1353


<210>  2980
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE CDS opt

<400>  2980
ucccaaaagg ucauauaccu uguaaugauc cuacucauag cuccagcaua cagcauacgc       60

ugcauaggcg uaucaaacag agacuucgua gaaggcauga gcggaggaac augggucgac      120

guaguacucg aacacggagg cugcgucaca guaauggcac aggacaaacc gaccguggac      180

auagaacucg ucacaaccac agucuccaac auggccgaag uaagaagcua cugcuacgaa      240

gcaagcauca gcgacauggc gucagacagc cgaugcccaa cacaaggcga agccuaccug      300

gacaaacaga gugacaccca guacgugugc aaacggacac uuguagacag aggcuggggc      360

aacggcugug gccuguucgg aaaagggagc cuagugaccu gcgcaaaauu cacaugcagc      420

aagaaaauga caggcaaauc aauccaacca gaaaaccucg aauacagaau aauguuaucc      480

guccacggaa gccaacacuc cggcaugaua guaaacgacg aaaaccgcgc caaaguagaa      540

guaacaccaa acucacccag agccgaagcc acgcucggcg gcuucggauc gcuaggccua      600

gacugcgaac cccgaacagg gcucgacuuc agcgaccucu acuaccucac gaugaacaac      660

aaacacuggc uaguacacaa agaaugguuc cacgacaucc cccuacccug gcacgcaggc      720

gcagauacag gcacaccgca cuggaacaac aaggaagcgc ucguggaguu caaagacgca      780

cacgccaaga gacaaaccgu agucguacua ggaagucagg aaggcgccgu acacaccgcc      840

cucgccggag cacucgaagc agaaauggac ggagcaaaag gaagacucuu cagcggacac      900

cucaaaugcc gccucaaaau ggacaagcua agacucaaag gcguaagcua cagccuaugc      960

acagccgccu ucacauucac aaaaguaccc gccgaaacac uccacggcac agucaccguc     1020

gaaguccaau acgccggcac agacggaccc ugcaaaguac ccgcacaaau ggccgucgac     1080

augcaaacgc ucacacccgu cggcaggcuc auaaccgcaa acccggucau caccgaaucc     1140

acggaaaaca guaaaaugau gcucgaacua gaucccccau ucggcgacuc guacauagua     1200

auaggcgucg gcgacaagaa aauuacacac cacuggcacc gauccggaag caccauaggc     1260

aaagccuucg aagcaacagu ccgcggcgcc aaacgcaugg ccguacuagg ggacacggca     1320

ugggacuuug gcucg                                                      1335


<210>  2981
<211>  1341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE CDS opt

<400>  2981
ucacaaaaag ucauauaccu aguaaugaua cugcuaauag caccagcaua uagcauacgc       60

ugcaucggag ucuccaacag agacuucguc gagggcauga gcgggggaac guggguggac      120

guaguacucg aacacggcgg augcgucacc gucauggcac aagacaaacc aacaguagac      180

auagaacugg ucacaaccac cgucucaaau auggccgaag ucagauccua cugcuacgaa      240

gcaagcaucu ccgacauggc aagcgacagc cgaugcccaa cccaaggcga agccuaccuc      300

gacaaacaau cagacacgca auacguuugc aaacgaaccc ucgucgaccg aggcuggggc      360

aacggcugcg gccucuuugg gaaaggaagc cuaguaacau gcgcgaaauu cacaugcucc      420

aagaaaauga ccggcaaauc aauccaacca gagaaccuag aauaccgaau caugcugagc      480

guacacggca gccaacacuc aggaaugaua ggguacgaaa ccgacgaaaa ucgagccaaa      540

gucgaaguca ccccaaacag cccacgagcc gaagccaccc ucggaggcuu cggaucacuc      600

ggacucgacu gcgaaccccg gaccgggcuc gacuucucag accuguacua ccucacaaug      660

aacaauaaac acuggcucgu acauaaagaa ugguuucacg acauaccccu ccccuggcac      720

gccggcgcag acaccggcac accacacugg aacaacaaag aagcacucgu ggaguucaaa      780

gacgcccacg ccaaacgcca gaccgucguc guacuaggcu cacaagaagg agcaguccac      840

acagcccuag caggggccuu agaagcggaa auggacggcg caaaagggaa acucuucagc      900

ggacaccuga aaugccgccu aaaaauggac aaacuacgac ucaaaggcgu aagcuacagu      960

cuaugcacgg ccgcauucac cuucaccaaa gucccagcag aaacacucca cggcacaguc     1020

acaguagagg uucaauacgc aggcacagac ggccccugca aaauacccgu ccaaauggcc     1080

guagacaugc aaacccuaac ccccgucggc agacuaauca cagccaaccc ggucaucaca     1140

gaauccacag aaaacagcaa aaugaugcuc gaacucgacc caccauucgg agacucuuac     1200

auagucauag gaguaggcga caagaaaauc acgcaccacu ggcaccgauc cggcuccaca     1260

auaggcaaag ccuucgaagc aaccgugcgc ggcgcaaaaa gaauggcagu ccucggagac     1320

accgccuggg acuucggcuc a                                               1341


<210>  2982
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE CDS opt

<400>  2982
ucgcagaaag ucauauaccu ggucaugauc cugcucauag ccccagccua cagucuacga       60

ugcaucggag uaagcaacag agacuucguc gaaggaauga guggaggcac auggguggac      120

gucguccugg aacacggcgg gugcgucaca guaauggcac aagacaaacc caccguagac      180

aucgagcuag ucacaaccac agucuccaac auggccgaag ucagaagcua cugcuacgaa      240

gcaagcauau cagacauggc aagcgacagc agaugcccga cccaggggga ggccuaccuc      300

gacaaacaaa gcgacaccca auacgucugc aaacgcaccc uaguggacag gggcuggggc      360

aauggcugcg gccuguucgg aaaaggcagc cuggucacau gcgcaaaauu cgccugcuca      420

aagaaaauga caggaaaaag cauccaaccc gaaaaccugg aauaucgcau aaugcuauca      480

guccacggca gccagcacuc aggcaugaua gugaacgaca ccggacacga aacagacgaa      540

aaccgcgcaa aaguagaaau aaccccaaac ucaccccgag ccgaagcaac gcuuggcggc      600

uucggaucac uuggacucga cugcgaacca agaaccggac uagacuucag ugaccucuac      660

uaccucacca ugaacaacaa acacuggcuc gugcacaaag aaugguucca cgacauacca      720

uuacccuggc acgcgggcgc agacacugga acaccccacu ggaacaacaa agaagcgcuc      780

guggaguuca aagaugccca cgccaaacgc cagaccgugg ucguacucgg gagccaggaa      840

ggagcaguac acacggcccu agcaggggcc cuagaagcag aaauggacgg agcaaaaggc      900

agacucucaa gcggacacuu aaaaugccgc cugaaaaugg acaaacuacg gcuaaaagga      960

guaaguuaca gccucugcac agccgcauuc acguucacga agauaccggc cgaaacacuc     1020

cacggaacag ucacgguaga aguccaauac gcgggcacgg acgggccaug caaaguaccc     1080

gcgcaaaugg caguagacau gcaaacgcua acacccgugg gccgacucau aacagcaaac     1140

cccgucauaa ccgaaagcac cgaaaacagc aaaaugaugc ucgaacucga ucccccguuc     1200

ggagacucau acauaguaau aggcguagga gaaaagaaaa ucacgcacca cuggcaccgc     1260

ucaggaucaa ccauaggcaa agccuucgaa gccacaguaa gaggggccaa acgcauggca     1320

gugcuaggcg acacagccug ggacuucgga uca                                  1353


<210>  2983
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE CDS opt

<400>  2983
agcaaaaaag ucauauaccu cgucaugauc cuccuuauag cccccgccua cucaauccgc       60

ugcaucggug uaagcaaccg cgacuucguu gaaggaaugu ccggcggaac cugggucgac      120

gucguccucg aacacggcgg cugcgugacu gucauggcac aagacaaacc caccguagac      180

aucgaacucg ucacaaccac cgucucgaac auggccgagg uacggagcua cugcuacgaa      240

gcauccauau cagacauggc cuccgacucc agaugcccaa cucaaggaga agccuaccuc      300

gacaaacaau ccgacaccca auacguaugc aaacgaaccc uaguggaccg aggauggggc      360

aacggcugcg gacucuucgg caaaggcucc cucgugacau gcgccaaauu cgccugcuca      420

aaaaaaauga ccggaaaauc cauccaaccc gaaaaccucg aauacaggau caugcuaagc      480

guccacggau cacaacacuc cggaaugauc gucaacgaca caggacacga aacagacgaa      540

aaccgagcaa aaguagaaau aacaccaaac ucccccagag ccgaagccac ccucggcggc      600

uucggcuccc uaggccuaga cugcgaacca agaacgggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa acacuggcuc guacacaaag aaugguucca cgauaucccc      720

cuccccuggc acgccggcgc cgacaccggg accccccacu ggaacaacaa agaagccuua      780

gucgaauuca aagacgcaca cgcaaaaaga caaaccgucg ucguccucgg aagccaagaa      840

ggcgcagucc acacagcccu cgcgggagcc cuagaagcag aaauggacgg agcgaaaggc      900

cgccucucca gcggccaccu caaaugccgc cucaaaaugg acaaacuaag acugaaaggc      960

gucuccuacu cccucuguac cgcagcauuc accuucacaa aaauccccgc agaaacccuc     1020

cacggcaccg ucaccguaga aguccaauac gcaggcacgg acggccccug caaaguacca     1080

gcccaaaugg ccguagacau gcaaacccua acccccgucg gccgacucau aaccgcaaau     1140

ccagucauca cagaaucgac cgaaaacagc aaaaugaugc ucgaacuuga ccccccauuc     1200

ggggacuccu acauaguaau cggcgucggc gaaaaaaaga uaacccacca cuggcaccgc     1260

ucuggaucua ccaucggcaa agccuucgaa gccaccguac gcggagcaaa acgcauggcc     1320

guccucggcg acaccgcaug ggacuucgga agc                                  1353


<210>  2984
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE CDS opt

<400>  2984
ucacaaaaag uaaucuaccu ggucaugauc cuacucauag cccccgcaua cagcauacgc       60

ugcauaggag ucucaaaccg cgacuucguc gaaggaauga gcgggggaac guggguagac      120

guaguccuag aacacggcgg augcguaacc guaauggcgc aagacaaacc aaccguagac      180

auagagcuag ucacaacaac agucucgaac auggcagaag ucaggagcua cugcuacgaa      240

gcaaguauaa gcgacauggc cagcgauucc cgaugcccga cacaaggaga agcauaccua      300

gacaaacaaa gcgacacaca auacguaugc aaacgcaccc uaguagaccg uggcuggggc      360

aacgguugcg gacuauucgg gaaaggaagu cuggucacau gcgcaaaauu cgcaugcagc      420

aagaaaauga ccggaaaauc gauacagccc gaaaaccuag aauaccgaau aaugcuaagc      480

guacacggga gccaacacuc gggaaugaua guaaacgaca ccggacacga gaccgacgaa      540

aacagagcaa aaguagaaau aacaccaaac ucgccucgag ccgaagcaac acucggaggc      600

uucggaucac ucggacucga cugcgagccc agaaccggcc ucgacuucuc ggaccuauac      660

uaccucacaa ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauaccc      720

cugcccuggc acgcaggcgc cgacacggga acgccccacu ggaacaacaa agaagcccua      780

guagaguuca aagacgcaca cgccaaacgc caaaccgugg ucgugcucgg gagccaagaa      840

ggagcagugc acacagcacu ggccggagcc cuggaagccg aaauggacgg agcaaaagga      900

aggcucucaa gcggacaccu aaaaugccgc cugaaaaugg acaaguuaag gcuaaaagga      960

guaucauaca gccucugcac ggccgcguuc accuucacaa aaauacccgc agaaacgcua     1020

cacggcaccg ucacagucga aguacaguac gcgggaaccg acggcccaug caaaguaccc     1080

gcacaaaugg ccguggacau gcaaacacua acgccagugg gcaggcuaau aaccgcaaac     1140

ccagucauaa cggaaagcac ggaaaauagc aaaaugaugc ucgaacucga cccaccguuc     1200

ggagacucgu acauaguaau aggcguaggc gaaaagaaaa uaacacacca cugguaccgc     1260

ucgggcucaa ccauaggcaa agcauucgaa gcaaccgugc gaggcgccaa acgcauggcu     1320

guacucggag acacagcaug ggacuucggc uca                                  1353


<210>  2985
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE CDS opt

<400>  2985
agccaaaaag ucauauaccu agucaugauc cuacuaaucg ccccagcaua cucaauacgc       60

ugcaucggcg ucagcaaucg agacuucguc gaaggcauga gcggcggcac augggucgac      120

guaguccucg aacacggcgg cugcgucacc gucauggccc aagacaaacc aaccguagac      180

auagaacucg ucacaacaac agucucaaac auggccgaag ucagaagcua cugcuacgaa      240

gccuccauaa gcgacauggc auccgacuca cgcugcccaa cacaaggcga agcauaccuc      300

gacaaacagu ccgacacgca auacguaugc aaacgcaccc uaguagacag gggcuggggg      360

aacggaugcg gcuuauucgg caaaggcagc cucgucacau gcgcgaaauu cgccugcucc      420

aagaaaauga ccggcaaauc cauacagcca gagaaccuag aauaccgaau aaugcucucc      480

guccacggau cacaacacag cggaaugauc guaaacgaca ccggccacga gaccgacgaa      540

aaccgcgcca aagucgaaau cacccccaac agcccacgag ccgaggccac acucggaggc      600

uucggcucgc uaggacucga cugugaaccu agaaccggcc ucgacuucuc agaccucuac      660

uaccuaacga ugaacaacaa acacuggcua gugcacaaag aaugguucca cgacauacca      720

cugcccuggc acgcaggcgc agacaccggc acgccacacu ggaacaacaa ggaagcccua      780

guagaguuca aagacgccca cgccaagcga caaaccgucg uagugcucgg cucccaagaa      840

ggagccgucc acacagcucu agccggagca cuagaagcag aaauggacgg agccaaagga      900

aaacucucaa gcggccaccu aaaaugccga cucaaaaugg acaaauuacg acuaaaaggc      960

guauccuacu cccuaugcac agccgcauuc acauucacaa aaauucccgc cgaaacgcua     1020

cacggcacag ucacagugga aguacaauac gccggcacag acggaccaug caaaguacca     1080

gcccaaaugg ccgucgacau gcaaacgcug acacccgucg gcagacucau aacagccaac     1140

cccgucauaa ccgaaaguac cgaaaacuca aaaaugaugc ucgaacucga cccaccauuc     1200

ggagacagcu acauagucau cggcgucgga gaaaagaaaa uaacccacca cuggcaccga     1260

agcggcucca caaucggaaa agccuucgaa gccacaguac ggggagccaa acguauggcc     1320

guacucggcg auaccgcaug ggacuucggc agc                                  1353


<210>  2986
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE CDS opt

<400>  2986
ucacaaaaag ucauauaucu agucaugauc cugcuaauag ccccagccua cagcauccgc       60

ugcaucggag uaucgaaccg cgacuucguc gaaggcauga gcggaggcac cuggguagac      120

guaguccuag aacacggcgg augcguuacc guaauggcac aagacaagcc aaccguagac      180

aucgagcuag ucaccaccac cguuuccaac auggcagaag uacgaagcua cugcuacgaa      240

gcaaguauaa gcgacauggc guccgacagc cggugcccca cacaaggcga agccuaccuc      300

gacaaacaaa gcgacacaca guacguuugc aagcggacgc ugguggaccg cggcuggggc      360

aacggaugcg gauuguucgg aaaaggaagc cuagucacau gcgcgaaauu cgcaugcagc      420

aagaaaauga caggcaaauc aauacagcca gaaaaccuag aauacagaau aaugcuaagc      480

guacacggaa gucaacacag cggcaugauc gucaacgaca caggccacga gaccgacgaa      540

aacagagcaa aaguagaaau aaccccaaac ucgccaagag ccgaagccac acucggagga      600

uucggcucgc ucggacucga cugcgagccc agaaccggac ucgacuucuc cgaccuauac      660

uaccucacaa ugaacaauaa acacugguua guacacaaag aaugguucca cgacauccca      720

cugcccuggc acgcaggagc cgacacaggc acaccccacu ggaacaacaa ggaagcucua      780

guagaguuca aagacgccca cgcgaaacgu caaaccguag uaguacuagg cagccaagaa      840

ggcgcagugc acacagcccu cgccggcgca cuagaagcgg aaauggacgg cgcaaaaggc      900

agauuaagca gcggccaccu aaaaugccgc cuaaagaugg acaaacuaag acucaaagga      960

guaagcuaca gccuaugcac agcagcauuc accuucacca aaauacccgc cgaaacacua     1020

cacgggacag uaacagucga aguacaguau gcaggcacag acggccccug caaaguaccc     1080

gcacaaaugg ccguggacac ccaaacacua acccccguag gcaggcuaau aaccgccaac     1140

ccaguaauca ccgaaagcac agaaaacagc aaaaugaugc ucgaacuaga ucccccauuc     1200

ggagacuccu acaucgucau aggagucggc gaaaagaaaa ucacucauca cuggcaccga     1260

ucaggcucca ccaucggcaa agcauucgaa gcaaccguac gcggcgccaa acguauggcc     1320

gugcuaggcg acacagccug ggacuucgga uca                                  1353


<210>  2987
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE CDS opt

<400>  2987
ucacaaaaag ucauauacuu agucaugaua cuacucauug cccccgcaua cuccauacga       60

ugcaucggcg ucuccaacag agacuucgua gaaggcauga gcggcggcac augggucgac      120

guaguccugg aacacggcgg cugcguaacc gugauggccc aagacaaacc aacuguagac      180

auagaacucg ucacaaccac cguaagcaac auggccgaaa uccgaagcua cugcuacgaa      240

gccagcauaa gcgacauggc guccgacagc cgcugcccaa cccaaggcga agccuaccua      300

gacaaacaga gcgacacaca auacgucugc aaacgcacac uaguagacag aggcugggga      360

aacggcugcg gacuauucgg aaaaggaagc cuaguaacgu gcgcgaaauu cgccugcagc      420

aaaaaaauga caggaaaauc aauccagccc gagaaccuag aauacagaau uaugcuaagc      480

guacacgggu cccaacacuc aggaaugaua guaaacgaca cuggccacga aaccgacgaa      540

aacagagcca aaguagaaau aacacccaac ucaccccgag ccgaagccac acuaggaggc      600

uucggcucac ucggacucga cugcgaacca agaaccggac uagacuucuc ggaccucuac      660

uaccucacga ugaauaacaa acacuggcuc guccacaaag aaugguucca cgacauaccc      720

cucccuuggc acgcuggcgc agacaccgga acaccacacu ggaacaacaa ggaagcacuc      780

gucgaauuca aagacgccca cgccaaacgc caaaccguag ucguacucgg aucccaagaa      840

ggggcaguac acacagcccu ggccggagca cucgaagcag aaauggacgg cgccaaagga      900

cgacuaucua guggccaccu gaaaugucgc cuaaaaaugg acaaacuccg acucaaaggc      960

guaagcuaca gccucugcac cgcagccuuc accuucacca aaauaccagc agaaacacua     1020

cacggcacgg uaaccgucga aguccaguac gcaggaacug acgggcccug caaaguccca     1080

gcccaaaugg cagucgacau gcaaacccuu acccccguag gacgccucau aacagccaac     1140

ccagucauca ccgaaagcac agaaaacagc aaaaugaugc ucgaacucga cccacccuuc     1200

ggagacucau acauagucau uggcgugggc gaaaaaaaaa uaacucacca cuggcaccga     1260

ucgggcucaa cuauaggcaa agccuucgaa gcgaccgucc gcggcgccaa acgcauggcc     1320

guacucggcg acacagccug ggacuucggc ucc                                  1353


<210>  2988
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE CDS opt

<400>  2988
agccaaaaag uaauauaccu cgucaugauc cugcucauag cccccgcuua cuccauacgc       60

ugcaucggcg ucagcaacag ggacuucgua gaaggcaugu caggcggcac auggguagac      120

guaguccuag aacacggggg augcgucacc guaauggccc aagacaaacc aacaguagac      180

aucgaacucg ucacgacgac cgucuccaac auggccgaag uacgcagcua cugcuacgaa      240

gcaagcauau cagacauggc auccgacucc cgcugcccca cccaaggcga agccuaccuc      300

gacaaacaau ccgacaccca auacguaugc aaacgaaccc uaguagaccg aggcugggga      360

aacggcugcg gacuauucgg aaaaggcuca cucguaacau gcgcaaaauu cgccugcagc      420

aagaaaauga ccggaaaauc cauccaaccc gaaaaccucg aauaccgcau aaugcucucc      480

guccacggaa gccaacacuc gggcaugauc guaaacgaca ccggccacga aaccgacgaa      540

aaccgcgcaa aaguagaaau aacacccaac agccccagag cagaagcaac ccucggcggc      600

uuuggauccc ucggacuaga cugcgaaccc cgaaccggcc ucgacuucag cgaucuauac      660

uaccucacaa ugaacaacaa acacuggcua guccacaaag aaugguucca cgacauaccu      720

cugcccuggc acgccggugc agacacaggc accccgcacu ggaacaacaa agaagcccua      780

gucgaguuca aagacgccca cgccaaacgc caaacagucg ucguccuagg cagccaagaa      840

ggcgcagucc acaccgcccu agcaggagcc cuagaagcag aaauggacgg agcaaaagga      900

agauuaucau ccggccaccu gaaaugucgc cuaaaaaugg acaaacuccg acucaaaggg      960

gucagcuaca gccucugcac ggccgcauuc acauucacaa aaaucccagc agaaacacuc     1020

cacggaaccg ucacaguaga aguccaauac gcaggcaccg acggccccug caaaguacca     1080

gcacaaaugg ccguagacau gcaaacccua acccccgucg gacgccuuau caccgcaaac     1140

ccaguaauca cggaaucaac cgaaaacagc aaaaugaugc uagaacucga cccgcccuuc     1200

ggcgacagcu acauaguuau aggcguagga gaaaagaaaa uaacccacca cuggcaucgc     1260

agcgguucaa ccaucggaaa agccuucgag gccaccguac gcggcgcacg ccgcauggcc     1320

guacucggcg acacggccug ggacuucgga agc                                  1353


<210>  2989
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE CDS opt

<400>  2989
agccaaaaag uaauauaccu agucaugaua cuacucaucg cacccgccua cucaauccgc       60

ugcauaggcg ucucaaaccg agacuucguc gaaggcauga gcggaggaac gugggucgac      120

guaguacucg aacacggcgg augcgugacc guuauggcac aagacaaacc aaccgucgac      180

auagaacucg ucacaacaac ggucucaaac auggccgaag uccgcucaua cugcuacgaa      240

gcauccauaa gcgacauggu aucugacuca cgcugcccaa cccagggaga agcguaccua      300

gacaaacaaa gcgacaccca auacgucugc aaaagaaccc uaguggaccg cggaugggga      360

aacggcugcg gccuauucgg caaaggaagc cuaguaacau gcgccaaauu cgccugcagc      420

aagaaaauga ccggcaaauc aauacagccc gaaaaccuag aauacagaau aaugcuaagc      480

guccacggcu cacaacacuc cggaaugauc guaaacgaca ccggacauga aaccgacgaa      540

aaccgagcaa aagucgaaau caccccaaac ucacccagag ccgaagccac ccuaggagga      600

uucggcagcc uaggccuaga cugcgaacca cgaaccggac ucgacuucuc cgaccucuac      660

uaccuaacaa ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacaucccc      720

cuccccuggc acgcaggagc agacaccggc acaccccacu ggaacaacaa agaagcgcuc      780

gucgaguuca aagacgcaca cgccaaacgc caaaccgucg ucguacuagg aagucaagaa      840

ggcgccguac acaccgcacu cgcaggcgca cuagaagcag aaauggacgg agccaaaggc      900

cgacucagca gcggacaccu caaaugccga cuaaaaaugg acaagcuacg ccucaaagga      960

gucuccuaca gccuaugcac agcagcguuu acauucacga aaaucccagc agaaacccuc     1020

cacggcacag ucacggucga aguacaauac gcaggcacag acggacccug caaagucccc     1080

gcgcaaaugg ccguagacau gcaaacccug accccaguag gccgccuuau caccgccaac     1140

ccagucauca ccgaauccac agaaaacucc aaaaugaugc ucgaacuaga ucccccguuc     1200

ggagacuccu acauaguaau aggcguagga gaaaagaaaa ucacccacca cuggcacaga     1260

ucgggcucca caaucggcaa agcauucgaa gcaaccgucc gaggcgccaa acgcauggcc     1320

guacucggag acacggcaug ggacuucggc uca                                  1353


<210>  2990
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE CDS opt

<400>  2990
agccaaaaag uaauauaccu cgucaugauc cuucucaucg caccagcaua uuccauacgc       60

ugcauuggag uaucaaacag agacuucguc gaaggaauga gcggaggcac guggguggac      120

gugguccuag aacacggggg cugcguaacc guaauggcuc aagacaaacc gacaguagac      180

aucgagcuag ucaccaccac cgucucaaac auggcagaag ucagaaguua cugcuacgaa      240

gccagcauaa gcgacauggc aagcgacagc agaugcccca cccaaggcga ggcauaccuc      300

gacaaacaaa gcgacaccca auacguaugc aaacgcacac ucguagaccg cggcuggggc      360

aacggcugcg gacuauucgg caaaggaagc cucgucacau gcgccaaauu cgcaugcuca      420

aagaaaauga ccggcaaaag cauacagccc gagaaccugg aauaccgaau aaugcucagc      480

guacacggau cacaacacuc aggcaugauc gugaacgaca cagggcauga aaccgacgaa      540

aaccgcgcca aaguagaaau caccccgaac uccccaagag ccgaagccac acucggcggc      600

uucggcucac uaggacucga cugcgagcca agaaccggcc uagacuucuc agaccuuuac      660

uaccucacaa ugaauaacaa acacuggcuc guccacaaag aaugguucca cgacauaccc      720

cuccccuggc acgcaggcgc agauacagga acgccccacu ggaacaacaa ggaagcacuu      780

guagaguuca aagacgcaca cgccaaacga caaaccguag ucguacucgg aagccaagaa      840

ggagccgugc acaccgcccu agcaggagcc cucgaagccg aaauggacgg agccaaaggc      900

agacuaucaa gcggacaccu aaaaugccgc cuaaaaaugg acaaacuacg acucaagggc      960

guauccuaca gccuaugcac cgcugcauuu accuuuacaa aaauacccgc cgaaacacua     1020

cacggcacag uaacagucga aguccaguac gcaggcacag acgggcccug caaaguacca     1080

gcacaaaugg caguagacau gcaaacgcua acgcccguag gacgccucau cacagccaac     1140

ccagucauca ccgaaagcau agaaaacagc aaaaugaugc uagaacuaga ucccccauuc     1200

ggagacucau acauagucau cggcguagga gaaaagaaaa ucacgcacca cuggcaccgc     1260

ucaggaucaa ccaucggcaa agccuuugaa gcaacagugc gaggcgccaa acgcauggcc     1320

guacucggag acacagccug ggacuucggc ucc                                  1353


<210>  2991
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE CDS opt

<400>  2991
ucgcaaaaag uaauauaccu agucaugaua cugcucauag caccagccua caguauacga       60

ugcaucggcg ucagcaacag ggacuucguc gaaggcauga gcggaggcac auggguugac      120

gugguacuag aacacggcgg augcguaacc gugauggcac aagauaaacc aacgguagac      180

aucgaacugg uaacaacaac aguaagcaac auggccgaag uaaggagcua cugcuacgaa      240

gcaagcauaa gcgacauggc aagcgacucc agaugcccaa cacaaggcga agccuaccuc      300

gacaaacaaa gcgacaccca auacgucugc aaacgcacac ucguagauag aggcugggga      360

aauggcugcg gccuauucgg aaaaggaagc cucgucacgu gcgcaaaauu cgccugcagc      420

aagaaaauga ccggcaaauc gauccaacca gaaaacuuag aauaucgaau caugcugagc      480

guacacggga gccaacacuc cggaaugaua gugaacgaca caggacacga aaccgacgag      540

aacagggcaa aaguagaaau cacaccaaac ucaccccggg ccgaagccac acucggagga      600

uucggaagcc ucggacuaga cugcgaacca agaacaggac ucgacuucag ugaccuauac      660

uaccucacaa ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauccca      720

cugccauggc acgcgggcgc agacacaggc acaccccacu ggaacaacaa agaagcccua      780

guagaguuca aagacgcaca cgccaaacgc caaaccguag ucguacuagg aagccaagaa      840

ggggccguac acacagcccu ggccggggca cucgaagcag aaauggacgg agcaaaaggc      900

aggcucucgu caggacaucu aaaaugccgc cuaaaaaugg acaaacucag gcucaaaggc      960

gugagcuaca gccuguguac agcagcauuc acauucacaa aaauacccgc agaaacacuc     1020

cacggcacag ucacgguaga aguacaauac gcaggcacag acggcccaug caaaguaccc     1080

gcgcagaugg ccgucgacau gcaaacacua acacccguag gacgacuaau aaccgcaaac     1140

ccggucauaa ccgaaagcac ggaaaauagc aaaaugaugc ucgaacuaga cccaccauuc     1200

ggcgauucau acaucgucau cggcauaggc gaaaagaaaa ucacacacca cuggcaccgc     1260

ucaggcucaa caauaggcaa agccuuugaa gcaaccguac gcggggcaaa acgcauggca     1320

gugcuagggg acacagcaug ggacuucggc agc                                  1353


<210>  2992
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE CDS opt

<400>  2992
ucacagaaag ucaucuaccu agucaugauc cuccuaaucg cacucgccua cucaauacgc       60

ugcauaggag ucuccaaccg agacuucgua gaaggaaugu ccggcggaac cuggguagac      120

guaguccucg aacacggggg cugcguaaca gucauggccc aagacaaacc caccgucgac      180

auagaacucg uaaccaccac cgucucaaac auggccgaag uccgaucgua cugcuacgaa      240

gccagcaucu ccgacauggc cuccgacucc cgaugcccaa cccagggaga agccuaccua      300

gacaaacaaa gcgacacaca auacguaugc aaacgaacac ucgucgaccg cggcugggga      360

aacgguugcg gacuauucgg caaaggaagc cuagucacau gcgcaaaauu cgcaugcuca      420

aagaaaauga ccggcaaauc cauccaacca gaaaaccucg aauaccgaau aaugcucagc      480

guccacggcu cacaacacag cggaaugaua guaaacgaca ccggacacga aaccgacgaa      540

aaccgcgcaa aagucgaaau cacccccaac uccccacgcg ccgaagccac ccuaggagga      600

uucggauccc ucggacuaga cugcgaaccc agaaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa acacugguua guccacaaag aaugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacacagga accccgcacu ggaacaacaa agaagcccuc      780

gucgaguuca aagacgcgca cgccaaacga caaaccgucg ucguacuagg gucccaagaa      840

ggagccguac acacagcacu agccggcgcc cuugaagcag aaauggacgg cgcuaaaggc      900

cgacucagcu ccggacaccu caaaugcaga cuuaaaaugg acaaacuccg ccucaaaggc      960

gucagcuaca gccucugcac cgccgcauuc acauucacaa aaauacccgc cgaaacccuc     1020

cacggcaccg ucacaguaga aguccaauac gccggaaccg acggccccug caaaguacca     1080

gcccaaaugg cagucgacau gcaaacacua accccagucg gacgacuuau cacggcaaac     1140

cccgucauca cagaaucaac cgaaaacucc aaaaugaugc ucgaacucga cccaccuuuc     1200

ggcgacuccu acaucguaau cggaguaggc gaaaagaaaa ucacacacca cuggcaccga     1260

uccggaucca cgaucggcaa agccuucgaa gcaacagucc gcggagcaaa aagaauggcc     1320

guacucggag auaccgccug ggacuucgga uca                                  1353


<210>  2993
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE CDS opt

<400>  2993
agccaaaaag ucauauaccu agucaugauc cuccucauag ccccagcaua cuccauacgc       60

ugcaucggag uaagcaaccg agacuucguc gaaggaaugu caggcggaac cugggucgac      120

gucguacuag aacacggcgg augcguaaca guaauggccc aagacaaacc aacaguagac      180

aucgaacucg uaacaaccac agucagcaac auggcagaag uaagauccua cugcuacgaa      240

gccucaauaa gcgacauggc auccgacucc aggugcccaa cacaaggcga agccuaccuc      300

gacaaacaaa gcgacacaca auacgucugc aaaagaacac uuguagacag aggauggggc      360

aacggaugcg gccucuucgg aaaaggaagc cucguaaccu gcgccaaauu cgccugcagc      420

aaaaagauga caggaaaauc gauccaaccc gaaaaccuag aauaccgcau caugcucucc      480

guccacggca gccagcacuc aggcaugaua gucaacgaca caggacacga aacagacgaa      540

aaccgagcca agguagaaau aaccccaaac agcccccgag ccgaagcaac ccucggagga      600

uucggaucac uaggacuaga cugcgaaccc cgaaccggcc ucgacuucuc cgaccuauac      660

uaccuaacca ugaacaacaa acacuggcuc guccauaagg aaugguucca cgacauaccc      720

cugcccuggc acgcgggcgc agacacgggc acaccccacu ggaacaacaa agaagcccuc      780

gucgaguuca aagacgccca cgcaaaacgc cagaccguag ucguacuagg cacccaagaa      840

ggagccgucc acaccgcccu ggccggagcc cucgaagccg aaauggacgg agcaaaaggg      900

cgacucagca gcggccaccu aaaaugccgc cuaaaaaugg acaaacuaag acuaaaaggc      960

guaucauaca gccuaugcac agcagcauuc accuucacca aaaucccugc agaaacacuc     1020

cacggcaccg ucacggucga aguccaguac gccggcacag acggacccug caaaguccca     1080

gcccaaaugg ccgucgacau gcaaacccua acacccgucg gaagacuaau aaccgccaac     1140

cccguaauca ccgaaagcac agaaaauuca aaaaugaugc ucgaacuaga ucccccguuc     1200

ggagacuccu acauaguuau cggcguaggc gagaaaaaga ucacacacca cuggcacaga     1260

uccggcagca caauaggaaa ggccuucgaa gcaacagucc gcggagcaaa acgaauggca     1320

guccucggag acacagccug ggacuucggc uca                                  1353


<210>  2994
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE CDS opt

<400>  2994
ucgcaaaaag ucaucuaccu agucaugauc cuccuaauag ccccggccua caguauacga       60

ugcaucggcg ucagcaacag ggacuucguc gaaggcauga gcgggggcac guggguagac      120

guaguuuuag aacacggcgg augcguaacc gugauggcac aagacaagcc aaccguagac      180

auagagcuag ucacaaccac aguuucaaac auggcagaag ucaggagcua cugcuacgaa      240

gcgagcauaa gcgacauggc gagcgacagc agaugcccaa cucaaggcga agccuaccuu      300

gacaaacaaa gcgacaccca guacguaugc aaaagaaccc uagucgaccg aggauggggg      360

aacgguugcg gacuauucgg aaaagggagc cuagucacau gcgcgaaauu cgccugcagc      420

aaaaagauga caggaaaaag cauccaaccc gagaaccuag aauaucgaau aaugcucagc      480

guacacggga gccaacacuc aggaaugaua gucaacgaca cagggcacga aaccgacgaa      540

aaccgagcga aaguggagau aacuccaaac ucacccagag cugaggcaac acuaggaggc      600

uucgggucac uuggacuaga cugcgaaccc agaacaggcc ucgacuucuc ggaccuauac      660

uaccuaacaa ugaacaacaa acacuggcuc gugcacaaag aaugguucca cgacauacca      720

cuaccuuggc acgcaggagc agacacaggu acaccacacu ggaacaacaa ggaagcacuc      780

guagaguuca aagacgcaca ugccaaacga cagaccguag ucguacucgg gagccaagaa      840

ggggccguac acacggcccu ggcaggagca cuagaagcag aaauggacgg agccaagggc      900

cgacucagca gcggccaccu caagugcaga uugaaaaugg acaagcuacg acucaaaggc      960

guaagcuaca gucuaugcac cgccgcauuc acauucacaa aaaucccagc agaaacgcua     1020

cacggcacag ucaccgucga aguucaguac gccggaacag acggaccaug caaagugcuc     1080

gcccagaugg cagucgacau gcaaacgcua acgcccguag guaggcucau aaccgcgaac     1140

ccgguaauca cagaaagcac agaaaacagc aaaaugaugc ucgagcucga cccacccuuc     1200

ggagacagcu acaucgucau agggguggga gaaaagaaaa ucacacacca cuggcaccgc     1260

agcgguucaa ccaucggcaa agccuucgaa gcgaccguac gaggagcuaa aagaauggcc     1320

guacucggag acacagccug ggacuucggc uca                                  1353


<210>  2995
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE CDS opt

<400>  2995
agccagaaag ucaucuaccu agucaugauc cugcucauag caccagcaua cagcauaaga       60

uguaucggag ucuccaacag agacuucguc gaaggaauga gcggaggaac cuggguagac      120

guagucuuag aacacggugg cugcgucacc gugauggcac aagacaaacc gacaguagac      180

auagaacucg ucacaacaac aguaagcaac auggcagaag uacgaagcua cugcuacgaa      240

gcguccauaa gcgacauggc cagcgacagc agaugcccaa cacaaggcga agcauaccua      300

gacaaacaau ccgacacaca guacgucugc aaacgcaccc uaguagacag aggguggggc      360

aacggaugcg gacuauucgg gaaagggagc cuagucacau gcgccaaauu cgccugcuca      420

aagaaaauga caggaaaaag cauacaacca gaaaaccuag aauaccgaau aaugcuaagc      480

guacacggaa gccaacacag cggcaugauc guaaacgaca ccggacacga aacagacgaa      540

aaccgagcaa aagucgaaau aacacccaac agcccccgag ccgaggccac acuaggcggu      600

uucggaagcc ucggacucga cugcgaacca cgcacagggc uagacuucuc cgaccucuau      660

uaccuaacaa ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauaccc      720

cuacccuggc acgcaggcgc ggacacagga accccgcacu ggaacaacaa agaagcccua      780

gucgaguuca aagaagccca cgccaaacga cagacaguag ucguccuagg aagccaagaa      840

ggagccgucc acacagcccu agcaggagca cucgaagcag aaauggacgg ggccaaagga      900

cgccuaagcu ccggacaucu aaaaugccgc cuaaaaaugg acaaacucag acuaaaagga      960

gucagcuaca gccuaugcac agccgccuuc accuucacca aaauaccggc agaaacacuc     1020

cacggcacag ucaccgucga aguccaauac gcaggcaccg acggaccaug caaaguaccc     1080

gcacaaaugg ccguagacau gcaaacacua accccagugg gacgacucau aacugccaac     1140

ccagucauca ccgaaucaac cgaaaacagc aaaaugaugc uagagcucga cccaccauuc     1200

ggagacagcu acauagucau cggcgucggc gagaagaaaa uuacgcauca cuggcacaga     1260

agcggcucga ccauaggcaa agccuucgaa gcaacaguac gcggagcaaa acgaauggcc     1320

guccucggag acacagccug ggacuucgga agc                                  1353


<210>  2996
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE CDS opt

<400>  2996
ucgcaaaaag ucaucuaccu aguaaugauc cugcuaauag caccagccua caguaucaga       60

ugcauaggag ucucgaacag ggacuucgua gagggaaugu cagggggcac guggguagac      120

gugguacuag aacacggcgg augcgugacc gugauggcgc aagacaaacc gaccguagac      180

auagaacucg ucacgacaac ggucucaaac auggccgagg ucaggagcua cugcuacgaa      240

gcgaguauaa gcgacauggc aagcgacagc agaugcccga cacagggcga ggccuaccuc      300

gacaaacaga gcgacaccca auacgugugc aaacgcaccc uaguggaccg aggcuggggc      360

aacggcugug gccuguucgg gaaggggagc cucgucacau gcgcaaaauu cgccugcagc      420

aagaaaauga caggaaaaag cauccagccc gaaaaccugg aauacagaau aauguuaagc      480

gugcacggaa gccagcacuc aggcaugauc guaaacgaca caggccacga gaccgacgaa      540

aaccgcgcaa aaguggaaau aaccccgaac ucgccgaggg cagaagccgc ccuaggggga      600

uucggcucgc uagggcuaga cugcgagcca agaacaggcc uggacuucag cgaccuauac      660

uaccuaacga ugaacaacaa acacuggcua gugcacaagg agugguucca cgacauaccc      720

uugcccuggc acgcgggagc cgacacagga acgccucacu ggaauaacaa ggaagcccua      780

gucgaguuca aagacgcaca cgcaaaacga cagaccguag uagugcuagg gagccaggaa      840

ggggcagugc acacggcucu ggccggagca cuggaagcgg aaauggacgg ggccaaaggc      900

aggcucucau ccgggcaccu gaaaugccgc cugaagaugg acaaacuaag acucaaaggc      960

gucagcuaua gccuaugcac ggccgcguuc acguucacga aaauaccagc cgaaacgcua     1020

cacggaacgg ucacgguaga aguccaguac gcgggcacgg acgggccaug caaagugccg     1080

gcgcaaaugg ccguagacau gcaaacgcuc acgccagugg gaaggcuaau aaccgcgaac     1140

ccaguaauaa cagaaagcac agaaaauagc aagaugaugc ucgagcucga ucccccguuc     1200

ggggacucau acauagucau aggcgugggc gagaaaaaga uaacgcacca cuggcacaga     1260

ucgggaucga ccauaggcaa agccuuugaa gcgaccguac gcggggcaaa acgcauggca     1320

guacucgggg acacggcaug ggacuuuggu ucg                                  1353


<210>  2997
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE CDS opt

<400>  2997
ucccagaaag ucauauaccu cguaaugauc cuacuaaucg caccagcaua cagcauaaga       60

ugcauaggcg uuuccaaccg agacuucgua gaaggcauga gcggcggcac auggguagac      120

gucguccucg aacacggagg augcguaacc gucauggccc aagacaaacc cacagucgac      180

aucgaacugg ucucuacaac agucuccaac auggccgaag uccgaagcua cugcuacgaa      240

gcaaccauau cggacaucgc auccgacagc cgcugcccga cacaaggcga agccuaccuc      300

gacaagcaaa gcgacacaca auacgucugc aaacgaacgc ucgucgaccg aggcuggggu      360

aacggcugcg gccucuucgg caaagggucc cucguaacau gcgccaaauu cgccugcuca      420

aagaaaauga caggcaaauc cauccaacca gaaaaccucg aauacagaau caugcuaucc      480

guacacggca gccaacacuc cggcaugauu guaaacgaca caggccacga aaccgacgaa      540

aaccgagcaa aaguagaaau caccccaaac agcccccgag cagaagccac ccucggcgga      600

uucggaagcc ucggccucga cugcgaaccc agaaccggac ucgacuucag cgaccucuac      660

uaccucacca ugaauaacaa acacuggcuc guacacaaag aaugguucca cgacaucccc      720

cuacccuggc acgcaggcgc cgacaccgga acaccccacu ggaacaacaa agaagcacuc      780

gucgaguuca aagacgccca cgccaaacgc cagaccgcag ucguccucgg aucacaggaa      840

ggcgccgucc auacagcccu agccggcgcc cuagaagcag aaauggacgg cgcgaaaggc      900

cgccucagca gcggccaccu aaaaugccgc cucaaaaugg acaaacuacg ccuuaaaggc      960

gucagcuaua gccucugcac agcugcauuc accuucacaa aaauccccgc agaaacccua     1020

cacggcacag ucaccgucga aguacaauac gccggcacag auggcccaug caaagugccc     1080

gcccaaaugg ccguggacau gcaaacccuc accccagucg gcagacucau aaccgccaac     1140

ccaguaauca ccgaaucaac cgaaaacucc aaaaugaugc ucgaacucga ucccccauuu     1200

ggagacuccu acauagucau cggcguaggc gaaaagaaaa ucacccacca cuggcaccgc     1260

agcggaucca ccauaggcaa agccuucgaa gccacagucc gaggagcaaa aagaauggcc     1320

gugcucggag acacagcaug ggacuucggc ucc                                  1353


<210>  2998
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE CDS opt

<400>  2998
ucacaaaaag ucauauaccu agucaugauc cuacuaauag caccagcgua uagcauacgc       60

ugcaucggag uaagcaacag ggacuucguc gagggcauga gcggaggcac auggguggac      120

gucguacugg aacacggagg augcguaaca gugauggcac aagacaaacc aaccguagac      180

auagagcuag uaacaacaac agucucgaac auggccgaag ucagaucaua cugcuacgaa      240

gccagcauaa gcgacauggc auccgacucc aggugcccaa cccagggcga agcauaccuc      300

gacaaacaaa gcgacacaca guacguaugc aaaagaaccc uaguggacag aggauggggc      360

aacggaugcg gacuauucgg aaaagggagc cucgucacau gcgcaaaauu cgccugcagc      420

aagaaaauga cagggaaauc aauacagcca gaaaaccuag aauaccgaau aaugcucagc      480

gugcacggaa gccaacacuc aggcaugauc guaaacgaca ccgggcacga aacagacgaa      540

aaccgcgcaa aagucgaaau aacaccaaac ucacccagag ccgaggccac acuuggggga      600

uucggcagcc ucgguuuaga cugcgaacca aggaccgggc ucgacuucuc agaccuguac      660

uaccucacaa ugaauaacaa acacuggcuc guacacaaag aaugguucca ugacaucccc      720

cuacccuggc acgccggcgc agacacaggc acgccacacu ggaacaacaa agaagcgcuc      780

guggaguuca aagacgccca cgccaaacgc caaaccguag uggugcuagg gagccaggaa      840

ggagcagugc acacagccuu agccggcgcc cuggaagcag aaauggacgg agcaaaaggc      900

cggcucagca gcggacaccu caaaugccga cugaaaaugg acaaacuacg ccuaaaagga      960

gugagcuaca gccucugcac agccgcguuc acauucagca agauacccgc ggaaacacua     1020

cacggaacag uaacagaaga aguccaauac gcaggcaccg acggacccug caaggugccc     1080

gcacaaaugg ccguagacau gcaaacacuc acaccgguug gcagacucau caccgcaaac     1140

cccgugauaa ccgaaagcac agaaaacuca aaaaugaugc ucgaacucga cccgccauuc     1200

ggagacucgu acauaguaau aggaguaggc gaaaagaaaa ucacgcacca cuggcaccgc     1260

agcggaucaa caaucggcaa agccuucgaa gcgaccguca gaggagcaaa acgcauggcg     1320

guacugggag acacagcaug ggacuucgga ucg                                  1353


<210>  2999
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE CDS opt

<400>  2999
ucccaaaaag uaauauaccu aguaaugauc cuccuaauag cacccgcaua cagcauccgc       60

ugcauagggg ucucgaacag ggacuucgua gaaggcauga gcggaggaac auggguagac      120

guagucuuag aacacggcgg cugcgucacc guaauggccc aagacaaacc aaccguagac      180

auagaacugg ucaccaccac gguaucgaac auggcagaag uccgaagcua cugcuacgaa      240

gccagcauca gcgacauggc cucagacagc cgaugcccca cccaaggcga agccuaccuc      300

gacaaacaau cagacaccca auacguaugc aaacgcaccc uaguagaccg aggcuggggc      360

aacggcugcg gccuguucgg aaaagggagc cuggucacau gcgccaaauu cgccugcucc      420

aagaaaauga caggaaaauc aauccaaccc gaaaaccuag aauaucgcau caugcuaucc      480

guacacggau cccaacacuc cggaaugaua guaaacgaca caggacacga aaccgacgaa      540

aaccgcgcca aaguagaaau aaccccaaac ucucccagag ccgaagccac acucggagga      600

uucggcuccc uaggccuaga cugcgaaccc cggaccgggc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa acacuggcuc guccauaaag aaugguuuca cgacauaccc      720

cucccauggc acgcaggcgc agacaccggu acaccacacu ggaacaacaa ggaagcgcua      780

guagaguuca aagacgccca cgccaaacgc caaaccguag ucgugcuagg cagccaggaa      840

ggagccguac acacagcacu ggccggagcu cucgaagcag aaauggacgg agccaaaggc      900

cggcuaucaa gcggacaccu aaaaugccgc cuaaaaaugg acaagcuaag acucaaaggc      960

gugagcuaca gccuaugcac agccgcauuc acguucacca aaauaccugc agaaacccua     1020

cacggaacag uaacagucga acuccaauac gcaggcacgg acggacccug caaagugccc     1080

gcccaaaugg ccguggacau gcaaacgcuc acacccguag gaaggcucau uacagcaaac     1140

ccagucauca ccgaaagcac agaaaacuca aaaaugaugc uagaacuuga ucccccauuc     1200

ggagacucgu acauagucau cggcguaggg gagaaaaaga ucacacacca cuggcaccga     1260

ucaggcucaa caaucggcaa ggccuucgaa gccaccgucc gaggagcaaa acgaauggcc     1320

guacuagggg acacagccug ggacuucggc uca                                  1353


<210>  3000
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE CDS opt

<400>  3000
agccaaaaag ucauauaccu ggucaugauc cuccuaauug caccagccua cagcauacgu       60

ugcaucggca ucuccaacag agacuuuguc gaaggcauga gcggagggac guggguagac      120

gugguacuag aacacggagg cuguguaacc guaauggccc aagacaaacc aaccgucgac      180

aucgagcuag ucacuacaac agucagcaac auggcagaag uccgaagcua cugcuacgaa      240

gccagcauaa gcgauauggc gagcgacagc agaugcccaa cccaagguga agcauaccua      300

gacaaacaaa gcgacaccca guacgugugc aaacgcacac ugguagaccg gggcuggggc      360

aacggcugcg gccuguucgg caaaggaucc cuagucacau gcgcaaaauu cgccugcagc      420

aagaaaauga ccggaaaauc uauccaacca gaaaaccuag aauaucgcau aaugcuaagc      480

guacacggaa gccagcacuc cggaaugauc guaaacgaca ccggacacga aaccgacgaa      540

aaccgagcca aaguagaaau cacccccaac ucgcccagag cagaagcaac ccuuggaggc      600

uucggaaucc uaggacucga cugcgaaccc aggaccggac uagacuucag ugaccuguac      660

uaccuaacaa ugaacaacaa acacuggcuc guccacaagg aaugguucca cgacauaccc      720

cucccuuggc acgcaggcgc agacacagga acgccacacu ggaacaacaa ggaagcacuc      780

gucgaguuca aagacgccca cgccaaacgu caaaccgucg uaguccucgg cucacaagaa      840

ggagccguac acacagcccu ggcaggagcc cucgaagcag aaauggacgg agcaaaagga      900

cgccuaagca gcgggcaccu aaaaugccgc cuaaaaaugg acaaacuccg acucaaaggc      960

guaagcuaca gccuaugcac cgccgcauuc accuucacaa aaauacccgc agaaacccuc     1020

cacggcaccg ucaccgucga aguccaauac gcaggaaccg acggaccaug caaagucccc     1080

gcacaaaugg caguagacau gcaaacccuc acaccaguag gaagacucau caccgcaaac     1140

ccagucauca ccgagagcac cgagaacuca aaaaugaugc ucgaacucga cccacccuuc     1200

ggcgacucuu auauagucau cgggguagga gaaaagaaaa ucacacacca cuggcaccga     1260

ucgggcucca ccauaggaaa agccuucgaa gcaaccgucc gcggagccaa acgcauggcu     1320

gugcucggag acacggcuug ggacuucgga uca                                  1353


<210>  3001
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE CDS opt

<400>  3001
agccaaaaag uaauauaccu cgucaugaua cuacuaauag ccccggcaua caguaucaga       60

ugcaucgggg ucagcaacag agacuucguc gaaggcauga guggaggcac guggguagac      120

guaguacuag aacacggagg augcgucaca gucauggcac aagacaaacc gaccguagac      180

auagagcuag uaacaaccac cgucagcaac auggccgaag ucagaagcua cugcuacgaa      240

gccaguauau ccgacauggc cagcgacucg aggugcccca cccaaggcga agccuaccuu      300

gacaaacaaa gcgacacaca auacguaugc aaaagaaccc uagucgaccg aggcugggga      360

aacggaugcg gccucuucgg aaagggaagc cuagucacau gcgccaaauu ugcaugcagc      420

aagaaaauga caggaaaaag cauacaaccc gaaaaccuag aauaccgcau caugcuaagc      480

guccacggaa gccagcauag cggcaugaua guaaacgaca cagggcacga aaccgacgaa      540

aaccgagcca aaguagaaau cacaccaaac agccccagag ccgaagccac gcucggaggc      600

uucggaagcc uaggacucga cugcgaaccc agaaccggac ucgacuucag ugaccucuac      660

uaccuaacaa ugaauaacaa acacuggcug guccacaaag aaugguucca cgacauccca      720

cuacccuggc acgcaggagc ggacacgggc acaccacacu ggaacaacaa agaagcucuc      780

guagaauuua aagacgccca cgcaaaacgc cagaccguag ucguccuagg aagccaagaa      840

ggagccguac acaccgcccu ggcaggagcg cuggaagcgg aaauggacgg aguaaaagga      900

aggcuaagca gcggccaccu caaaugccgc cuaaaaaugg acaaacuacg ccuaaagggc      960

guaagcuaca gccuaugcac agccgcguuc acauucacaa agauaccagc cgaaacgcuc     1020

cacgggacag ucacaguaga aguccaauac gcaggaacag acggaccaug caaagugcca     1080

gcacaaaugg ccgucgacau gcaaacccua acccccguag gccggcuaau aacagcaaac     1140

ccugucauaa ccgaaucgac agaaaacucc aagaugaugc uagaacucga ucccccauuc     1200

ggcgacaguu acauugucau aggagugggc gaaaagaaaa uaacacacca cuggcaucga     1260

agcggaucaa ccaucggcaa agccuucgaa gccaccguac gaggagccaa acgcauggca     1320

gugcuaggcg acacagcaug ggacuucggc agu                                  1353


<210>  3002
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE CDS opt

<400>  3002
agccaaaaag uaauauaccu agucaugauc cuacucauag ccccagcaua cagcauacgc       60

ugcauaggag uguccaaccg agacuucguc gaaggcaugu caggcggcac guggguagac      120

guaguacuag aacacggcgg gugcguaaca gugauggccc aagacaaacc cacagucgac      180

aucgagcucg ucacaaccac agucuccaac auggccgaag ucagaucaua uugcuacgaa      240

gcaagcaucu ccgacauggc aagcgacagc agaugcccaa cccaaggaga agccuaccuu      300

gacaaacaau cagacaccca auacgucugc aaacgcaccc uagucgaccg gggauggggc      360

aacggaugcg ggcuauucgg aaaaggaucc cuaguuacau gugccaaauu cgcaugcuca      420

aagaaaauga ccgggaaauc aauacaaccc gaaaaccuag aauaccgcau caugcugagc      480

guacacggca gccaacauuc aggcaugauc guaaacgaca ccggacacga aacagaugaa      540

aaccgcgcca aaguggaaau aacuccaaac ucccccagag ccgaagccac acucggaggc      600

uucggcucgc ucggacucga cugcgaaccc agaacaggcc uugacuucuc agaccuauau      660

uaccuuacaa ugaacaacaa acacuggcuc guacacaaag aaugguucca cgauauaccc      720

cuacccuggc acgcaggcgc agacaccggc acaccccacu ggaacaacaa agaagcccua      780

guagaguuca aagacgcaca cgcaaaacgc caaaccgucg uaguccucgg aucacaagaa      840

ggcgccguac acacggcccu ugccggcgca cuagaagcag agauggacgg cgccaaaggc      900

cgacuaucaa gcgggcaccu gaaaugcaga cucaaaaugg acaaacuccg acuaaagggc      960

guguccuacu cacuaugcac agcagcauuc acauucacaa agauacccgc agaaacccua     1020

cacggaacgg uaacagucga aguacaauac gccggcgcag auggaccaug caaaguaccc     1080

gcgcaaaugg ccguagacau gcaaacacuc acgcccgucg gacgacucau aacugcgaac     1140

cccgucauaa cagaaagcac agaaaacucc aaaaugaugc uagaacucga ucccccauuc     1200

ggcgacuccu auauagucau cggcgucgga gagaagaaaa uaacacacca cuggcaccgc     1260

uccggcucca cuaucggcaa agccuucgaa gccaccguac gaggagccaa acgaauggcc     1320

guccucggag acaccgcaug ggacuuugga uca                                  1353


<210>  3003
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE CDS opt

<400>  3003
ucccagaaag ucaucuaccu gguaaugauc cuacuaauag cccccgcaua cagcauaaga       60

ugcaucggag uauccaacag agacuucguc gaaggaaugu ccggcggcac auggguagac      120

guagucuugg aacacggagg cugcguaacc gucauggcac aagacaaacc caccgucgac      180

aucgaacucg ucacgaccac agucucgaau auggccgaag ucagaagcua cugcuacgaa      240

gcaagcauaa gcgacauggc cagcgacagc agaugcccaa cccaaggcga agccuacuua      300

gacaaacagu ccgacaccca auacguaugc aaacgcaccc ucguagaccg aggcugggga      360

aacggcugcg gccuguucgg aaaaggaagc cucguaaccu gcgcgaaauu cgccugcucc      420

aagaaaauga ccggaaaauc aauccagccu gaaaaccucg aauaccgcau aaugcucagc      480

guccacggaa gccagcauag cggcaugauc guaaaugaca caggacacga aaccgacgaa      540

aaccgcgcaa aaguagaaau aacaccgaac ucccccaggg ccgaggccac acucggcggc      600

uucggcucgc ucggccucga cugcgaacca aggaccggac uagacuucag cgaccuauac      660

uaccucacaa ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauaccc      720

cucccauggc acacaggcgc cgacaccggc acaccccacu ggaacaacaa agaagcccua      780

guagaguuca aagacgccca ugccaaacgc caaaccgugg ucguccucgg aucacaggaa      840

ggcgccguuc acaccgcucu agcaggagcc cuagaagccg aaauggacgg agccaaggga      900

cgacucucaa gcggacaccu caaaugcaga cucaaaaugg acaagcuacg acuaaaagga      960

guaagcuaua gccugugcac cgcagcauuc accuucacca aaauacccgc agaaacacuc     1020

cacggcacgg uaaccgucga aguacaauac gcaggcaccg acggcccaug caaaguaccc     1080

gcccaaaugg ccgucgacau gcaaacacua acccccguag gccgacucau aacagcaaac     1140

ccagucauaa ccgaaggcac cgaaaauagc aagaugaugc ucgaacugga cccgcccuuc     1200

ggcgacucgu acauagucau aggagugggc gagaaaaaga ucacacacca cuggcaccgc     1260

uccggcucaa ccauaggcaa agccuucgaa gccaccgucc gaggggccaa acgcauggcc     1320

gugcucggag acacagccug ggacuucggc uca                                  1353


<210>  3004
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE CDS opt

<400>  3004
ucgcaaaaag uaauauaccu cgucaugauc cuccucauag cccccgccua cuccauacgc       60

ugcauaggcg ucuccaaccg ggacuucguc gaaggaauga gugggggcac cugggucgac      120

guaguccuag aacacggcgg augcguaacc gcaauggccc aggacaagcc caccgucgac      180

auagaacucg uaacgacaac agucuccaac auggccgaag ucagauccua cugcuacgaa      240

gcaagcaucu cagacauggc aagcgacagc cgaugcccaa cccaaggcga agccuaccua      300

gacaaacaaa gcgacaccca auacguaugc aaacgaaccc uagucgaccg cggcuggggc      360

aacgggugcg gccuauuugg gaagggaagc cucgucacau gcgcaaaauu ugcaugcagc      420

aaaaagauga ccgggaaaag cauccaacca gaaaaucucg aauaccgcau aaugcuaucc      480

guccacggca gccaacacag cggcaugcua guaaacgaca ccggacacga aaccgacgaa      540

aaccgcgcaa aaguagaaau aacaccaaac uccccacgag cagaagcaac acucggcggc      600

uucggaagcc uaggccuaga cugcgaaccc agaaccggac ucgacuucag cgaccucuac      660

uaccucacaa ugaacaacaa acacuggcua gcccacaagg aaugguucca cgacauccca      720

cuacccuggc acgcaggcgc agcaacggga acaccacacu ggaacaacaa agaagcccua      780

guagaguuca aagacgcaca ugcuaaacga caaaccgucg uaguacuagg aagccaagaa      840

ggcgcagucc acaccgcccu cgccggagca cuagaagccg aaauggacgg agccaagggg      900

agacuuucaa gcggccaccu aaaaugucgc cuaaaaaugg acaaacuacg ccucaaaggc      960

gucagcuaca gccucugcac agccgcauuc acauucacca aaauaccugc ugaaacaguc     1020

gacggaacag uaacagucga aggacaauac ggaggaacug acggccccug caagguacca     1080

gcacaaaugg ccgucgacau gcagacgcua acgccaguag gacgacucau aaccgccaac     1140

ccagucauaa ccgaauccac agaaaacagc aaaaugaugc ucgaacuaga cccacccuuc     1200

ggggacuccu acauagucau aggagucggc gaaaagaaaa ucacccacca cuggcaccgc     1260

agcggcucaa ccauaggcaa agcauucgaa gcgacaguac gcggagcaaa acgcauggcc     1320

guacuaggag acacagccug ggacuucgga ucc                                  1353


<210>  3005
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE CDS opt

<400>  3005
ucccaaaagg ugauauaccu agucaugauc cuccuaauag ccccagccua cagcauacgg       60

ugcaucggag ucuccaacag agacuucguc gaaggcauga gcggaggaac auggguagac      120

guaguccuag aacacggcgg augcguaaca gugauggcgc aagacaaacc agccguagac      180

auagaacugg ucacaacaac ggucagcaac auggcagaag ucagaagcua cugcuacgaa      240

gcaagcauaa gcgacauggc gagcgacagc agaugcccaa cccagggcga agcauaucuc      300

gacaaacaau cagacacaca guacguaugc aagagaacgc ucguagaccg aggcuggggc      360

aacggaugcg gccuguucgg aaaggggagc cucgucacgu gcgcgaaauu cgcgugcagc      420

aagaaaauga ccgggaaauc aauacagcca gagaaccugg aauaccgaau aaugcucagc      480

guccacggca gccaacacag cggcaugauc gucaacgaca caggacauga aaccgacgaa      540

aaucgcgcaa aagucgaaau cacaccgaac ucgcccagag ccgaagccac acucggggga      600

uucgguagcc uaggacucga cugcgagccc aggacaggac ucgacuucuc ggaccuguac      660

uaccuaacaa ugaacaacaa acacuggcuc gugcacaaag aaugguucca cgacauaccc      720

cuacccuggc acgcaggcgc agacacaggc acaccccacu ggaacaacaa agaagcccua      780

guggaguuca aagacgcaca ugcaaaacga caaaccguag uagugcuagg aagccaggaa      840

ggagccguac acacagcccu ggccggagca uuggaagccg aaauggacgg agcaaaaggc      900

aggcucucca gcgggcaccu gaaaugccgc cugaaaaugg acaaacuacg acucaaaggc      960

guguccuaca gucucugcac cgcagcguuc acauucacaa aaauacccgc agaaacgcuc     1020

cacggcacgg ucacggucga aguccaauac gcuggaaccg acggacccug caaaguacca     1080

gcgcaaaugg ccguggacau gcagacgcuc acaccagugg gacggcucau aacagcgaac     1140

ccagucauaa cagaaagcac agaaaacagu aaaaugaugc ucgaacuaga cccgcccuuc     1200

ggggacuccu acauaguaau cggcguaggc gaaaagaaaa uaacgcacca cuggcaccgc     1260

uccggcagua ccauaggaaa agcauucgaa gcgacaguac gaggagccaa gcgcauggcg     1320

guacugggag acacagccug ggacuucggc ucc                                  1353


<210>  3006
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE CDS opt

<400>  3006
agccaaaaag ucaucuaccu uguaaugauc cuccuaauag ccccagcgua cagcauccgc       60

ugcaucggcg uaaacaacag ggacuucgua gaaggcaugu ccggaggcac cuggguagac      120

gucguacucg aacacggugg augcguaacc guaauggccc aagacaaacc aaccguagac      180

aucgagcuag ucacgaccac aguaagcaac auggcagaag ucaggagcua cugcuacgaa      240

gcaagcauca gcgacauggc aagcgacagc cgcugcccaa cccaaggaga agcguaccuc      300

gacaaacaaa gcgacacaca auacgucugc aaaagaaccc ucguggaccg aggcuggggc      360

aacggcugug gccuauucgg caaagggagc cuagucacau gcgccaaauu cgcaugcagc      420

aagaaaauga ccggaaaaag cauccaaccc gaaaaccuag aauaccgaau aaugcugagc      480

guccacggaa gccaacacuc aggcaugauc gucaacgaca ccggccacga aacagacgaa      540

aacagagcaa aaguagaaau caccccaaac agccccagag ccgaagcaac acuaggcggc      600

uucggaagcc ugggacugga cugcgagccc cgaaccggac uagacuucuc agaccuauac      660

uaccucacaa ugaauaacaa acacuggcua guacacaaag aaugguucca cgacauacca      720

cugccauggc acgccggcgc ggacaccgga acaccccacu ggaacaacaa agaagcacuc      780

guagaguuca aagacgccca cgccaaacgc caaaccgucg uaguacuagg cagccaagaa      840

ggagccguac acacagcccu ggccggagca cucgaagccg aaauggacgg ggccaaaggc      900

cgacucucca gcgggcaccu caaaugccgc cucaaaaugg acaaacuacg acucaaaggc      960

guaagcuaca gccucugcac agccgcauuc acauucacca aaauacccgc cgaaacccua     1020

cacgguacag ugacagucga aguacaauac gcaggcacgg acgggcccug caaaguaccc     1080

gcacagaugg ccguagacau gcaaacacua acaccagucg gcagacucau cacagccaac     1140

cccguuauca ccgaaagcac agaaaacagc aaaaugaugc ucgaacuaga cccaccauuu     1200

ggcgacagcu acauagucau uggagucgga gaaaagaaaa ucacacacca cuggcacaga     1260

uccggcagca caaucggaaa agccuucgaa gcaaccgucc gaggcgcgaa acgaauggca     1320

guacuaggag acacagcaug ggacuucggu agc                                  1353


<210>  3007
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE CDS opt

<400>  3007
ucccaaaaag uaauauaccu aguaaugaua uuacucauag caccggccua cagcauccgc       60

uguauagggg uauccaacag agacuucguc gagggcauga gcggcggcac augggucgac      120

gucguacuag agcacggcgg cugcguaaca gucauggcac aagacaaacc aaccguggac      180

aucgagcuag ucaccaccac agucucaaac auggccgaag ucagaagcua cugcuacgaa      240

gcgagcauca gcgacauggc aagcgacagc agaugcccaa cccaaggcga ggcuuaccuc      300

gacaagcaga gcgacaccca auacgugugc aaacgcacgc uaguagacag aggcugggga      360

aauggaugcg gacuuuucgg aaaaggaagc cuaguuacgu gcgcaaaauu cgccugcagc      420

aagaaaauga ccgggaaauc aauccaaccc gaaaaccuag aauaccgaau aaugcuaagc      480

guacacggca gccaacacuc gggaaugaua guaaacgaca caggacacga gacagacgaa      540

aacagagcca aaguagaaau aacaccgaac ucacccaggg ccgaggcaac acucggaggc      600

uucggcucgc uagggcucga cugcgagccc agaaccggcc uagacuucuc cgaccuauac      660

uaccucacaa ugaauaacaa acacuggcua guacacaagg aaugguucca cgacauacca      720

cugcccuggc acgcaggcgc agacacagga acaccccacu ggaacaacaa ggaagcccua      780

gucgaguuca aagacgcaca cgcaaagcga caaaccguag ucguccuugg aagccaagaa      840

ggagccguac acacagcccu cgcaggggcc cuggaagcag aaauggacgg agccaaaggc      900

agacuaucca gcggacaccu gaagugccga cucaaaaugg acaagcucag gcucaaggga      960

gucuccuaca gccuaugcgc agccgccuuc acauucacaa aaauacccgc ggaaacgcuc     1020

cacggcacag uaacggucga aguccaguac gcaggcacag acggacccug uaaaguaccc     1080

gcgcaaaugg cagucgacau gcaaacacua acaccagucg gcaggcucau aaccgccaac     1140

ccggucauaa ccgagaguac agaaaacagc aaaaugaugc ucgaacucga ucccccauuc     1200

ggagacagcu acauaguaau aggaguaggc gaaaagaaaa ucacccacca cuggcacaga     1260

ucaggcucaa caauaggcaa agcauucgaa gcaaccguac gcggagcaaa acgaauggcc     1320

guccuagggg acaccgccug ggacuuuggu uca                                  1353


<210>  3008
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE CDS opt

<400>  3008
ucacaaaaag uaaucuaccu cguaaugauc cuacuaaucg cacccgccua cagcauacga       60

ugcaucggag uaucaaaccg cgacuucguc gaagguaugu ccggcggaac auggguagac      120

gucgucuuag aacacggagg gugcguaaca gucauggccc aagacaaacc aacaguagac      180

auagagcucg uaacaaccac cguaagcaac auggcagaag uccgcagcua cugcuacgag      240

gccagcauau cagacauggc cuccgacuca cgaugcccaa cacaaggaga agcauaccua      300

gacaaacaaa gcgacaccca auacgucugc aaaagaacac uggucgacag aggauggggc      360

aacggaugcg gccuauucgg aaaaggcagc cuaguaaccu gcgcaaaauu cgccugcagc      420

aagaaaauga ccggcaaauc cauacaacca gaaaaucuag aauaccgaau aaugcuaucc      480

guccacggaa gccaacacuc cggcaugauc gucaacgaca ucggccacga aaccgacgaa      540

aaccgagcca aagucgaaau cacccccaac ucaccccgag cagaagccac acucggcggc      600

uucggauccc ucggacuaga cugcgaaccc agaacaggcc ucgacuucag cgaccucuac      660

uaccuaacaa ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauacca      720

cugcccuggc acgcaggcgc cgacacaggc acgccccacu ggaacaacaa agaggcacuc      780

guggaguuca aagacgccca cgccaaaaga caaacaguag uaguacucgg cagccaagaa      840

ggagccgucc acacagcccu cgcaggagcc cucgaagccg aaauggacgg agccaaaggc      900

agacucucca gcgggcaccu gaaaugccgc cucaaaaugg acaaacuacg ccucaaaggc      960

gucagcuaca gccucugcac agcagccuuc accuucacca aaauccccgc cgaaacacuc     1020

cacggaaccg uaacaguaga aguccaauac gccggcacag acggacccug caaaguaccc     1080

gcacaaaugg ccgucgacau gcagacccuc acgcccguag gaagacucau caccgcaaac     1140

cccgucauaa ccgaaagcac agaaaacagc aagaugaugc uagaacucga cccacccuuc     1200

ggagacagcu acaucguaau aggaguggga gacaagaaaa ucacacauca cuggcaccgc     1260

agcggcucaa caauaggcaa agcauuugaa gccacaguac gcggggccaa aagaauggcc     1320

guccuagggg acaccgccug ggacuucgga ucc                                  1353


<210>  3009
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE CDS opt

<400>  3009
agucaaaaag ucauauaccu cgucaugauc cugcuaaucg cccccgccua cagcauacgc       60

uguaucggag ugagcaacag ggacuucgua gaaggaauga guggaggcac cugggucgac      120

guaguacuag aacacggagg augcguaacc guaauggcac aagacaaacc aaccguagac      180

aucgagcuag ucacaaccac agucucaaac auggccgaag ucagaagcua cugcuacgaa      240

gcaagcauca gcgacauggc aagcgacagc cgaugcccaa cacaaggaga agcauaccua      300

gacaaacaaa gcgacaccca auacgugugc aaacgcaccc uagucgaccg aggauggggc      360

aauggaugcg gccucuucgg aaaaggaucc cuagucacau gcgcgaaauu cgcaugcagc      420

aagaaaauga cuggaaaauc aauccagcca gaaaaccuag aauaccgaau caugcucucg      480

guacacggca gucaacacag cgguaugaua gugaacgaca ccggacacga aaccgaugaa      540

aacagagcca aagucgaaau aaccccaaac ucccccagag ccgaagccac acuaggaggc      600

uucggaucgc uaggacuuga cugcgaacca agaaccggac uagacuucuc cgaccuauac      660

uaucuaacaa ugaacaacaa acacuggcuc gugcacaaag aaugguucca cgauauacca      720

cugccauggc acgcaggagc ggacacagga acaccccacu ggaacaacaa agaagcccua      780

guagaguuca aagacgccca cgccaaacga caaaccgucg ucguacuagg cagccaggaa      840

ggagccguac acacagcccu cgcgggcgcc cuagaagccg aaauggacgg agcaaaaggc      900

agacuaucgu caggccaccu caaaugccgc cugaaaaugg acaaacuaag gcucaaagga      960

gugucauaca gccucugcac agccgcguuc acauucacaa aaauaccagc agaaacacuc     1020

cacggaacag uaacaguaga aguccaauac gcaggaacag acggacccug caaagucccc     1080

gcccaaaugg ccguagacau gcaaacccuc acgccagucg gcagacugau aaccgcaaac     1140

ccaguaauca cagaaagcac agaaaacuca aaaaugaugc ucgaacuaga cccaccauuc     1200

ggcgacagcu auauaguaau aggcgucgga gacaagaaaa ucacacacca cugguaccgc     1260

ucaggcucca ccaucggaaa agccuucgaa gccaccguac gaggagcaaa acgcauggca     1320

gugcuagggg acacggccug ggacuucgga agc                                  1353


<210>  3010
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE CDS opt

<400>  3010
agccaaaaag uaauauaccu aguaaugauc cuacuaauag cccccgcaua cagcauccgc       60

ugcauagggg uauccaacag ggacuucguc gaaggcauga gcggaggcac augggucgac      120

gugguccuag agcacggagg cugcguaacc guaauggcac aagacaaacc aaccgucgac      180

aucgaacugg ucaccaccac aguuagcaac auggccgaag ucagaagcua cugcuacgaa      240

gcaagcaucu cagacauggc aagcgacucc cggugcccca cacaaggaga agcauaccua      300

gacaaacaga gcgacaccca auacguaugc aaacgcacac ucgucgaccg aggcuggggc      360

aacggcugcg gacuauucgg aaaaggaagc cucgucacau gcgcaaaauu cacaugcagc      420

aaaaaaauga caggaaagag cauccaacca gaaaaccuag aauaccgcau aaugcuaagc      480

guacacggua gccaacacuc aggcaugaua gucaacgaca ccggccacga aaccgacgaa      540

aaccgagcca aaguagaggu cacccccaac agcccacgag ccgaagcaac ccuaggcggc      600

uucggcagcu uaggccucga cugcgaaccc cgcaccgggc uagacuucag cgaccuauac      660

uaccuaacca ugaacaacaa acacuggcuc gugcacaaag aaugguucca cgacauaccc      720

uuaccauggc acgccggcgc ggacacaggc acaccccacu ggaacaacaa agaagcccuc      780

gucgaauuca aagacgcuca cgccaaacgc caaacaguag uaguacuagg cagccaggaa      840

ggcgcgguac acaccgcccu agccggagcc cuagaagccg aaauggacgg ggccaaaggc      900

cgacucuuca gcggacaccu gaaaugccgg cugaaaaugg acaaacucag acuaaaaggc      960

gucagcuaca gccuaugcac agcagccuuc acauuuacaa aaguacccgc agaaacacuc     1020

cacggcaccg ucacagucga aguacaauac gcaggcacag acggcccaug caaaguacca     1080

gcacaaaugg ccguagacau gcaaacacuc acaccagugg gcagacucau caccgccaac     1140

ccaguaauaa ccgaaagcac cgaaaacucc aaaaugaugc uagaacucga cccaccauuc     1200

ggcgacuccu acauaguaau aggcgucggc gacaaaaaaa ucacacacca cuggcaucga     1260

agcggcucca caaucggcaa agcauucgaa gcaaccguaa gaggagccaa acgaauggcu     1320

guacucggcg acaccgcaug ggacuucggc uca                                  1353


<210>  3011
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE CDS opt

<400>  3011
ucgcaaaaag ucaucuaccu aguaaugauc cuccucauag ccccagcuua cagcaucaga       60

uguaucggag uauccaacag agacuucguc gaaggcauga gcgggggcac augggucgac      120

guaguccuag agcacggcgg gugugucacu guaauggcgc aagacaaacc aacaguagac      180

auagagcuag uaacaaccac cgucucaaac auggccgaag uccgaagcua cugcuaugaa      240

gccagcauaa gcgacauggc aagcgacagc agaugcccga cacaaggcga agccuaccua      300

gacaaacaaa gcgacaccca auacguaugc aaacgcaccc uaguagaccg gggaugggga      360

aacggcugcg gccuauucgg gaaaggaagc cuaguaacau gcgccaaauu cacaugcagc      420

aagaaaauga ccgggaaauc aauccaaccc gaaaaccuag aauaccgcau aaugcugagc      480

gugcacggaa gccaacacuc aggcaugaua guaaacgaca ucggccacga aacagacgaa      540

aaccgagcga aaguagaagu gacaccgaac ucacccaggg cagaagccac gcucggaggc      600

uucgguucgu uaggacuaga cugcgagccc agaacaggac ucgacuuuuc ggaccuguac      660

uaccuaacca ugaacaacaa acacuggcug guacacaaag aaugguucca cgacauaccc      720

cugccauggc acgcaggagc agacacuggc acaccccacu ggaacaacaa agaagcacuc      780

guggaguuca aagacgccca cgccaaacga caaaccgucg ucgugcucgg aagucaagaa      840

ggagcagugc acaccgcccu cgccggagca cuagaagcgg aaauggacgg ggccaaaggc      900

cgccucuuca gcggacaccu caaaugcagg cugaaaaugg acaaacuaag acucaaggga      960

guaagcuaca gccucugcac cgcugcauuc accuucacga aaguacccgc ggaaacgcua     1020

cacggaacag uuacggucga aguccaguac gcaggcacgg acgggcccug caaagugccc     1080

gcacagaugg ccguugacau gcaaacacuc accccagucg gcagacucau aacugcaaac     1140

ccggucauca ccgaaaguac ggaaaacagc aagaugaugc uagagcuaga ucccccauuc     1200

ggggacuccu acauagucau cggcgugggc gacaagaaaa ucacgcacca cuggcaccga     1260

ucgggcucga ccaucggcaa agccuuugaa gcaaccgugc gaggagccaa acgcauggcc     1320

guccucggcg acacagccug ggacuucggc ucc                                  1353


<210>  3012
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE CDS opt

<400>  3012
ucccaaaaag uaauauaccu cgucaugaua cuccucauag cccccgcaua cagcauacgc       60

ugcaucgggg ucuccaacag agacuucguc gaaggaauga gcggaggcac auggguggac      120

guaguccugg aacacggagg augcguaacc guaauggccc aagacaaacc aaccgucgac      180

auagagcuag uuacaaccac cgucuccaac auggccgaag uccgcuccua cugcuacgaa      240

gccagcauaa gcgacauggc cagcgacagc agaugcccga cccaaggcga agccuaccua      300

gacaaacaaa gugacaccca auacgucugc aaacgaacac ucguagaucg cggcuggggc      360

aacggcugcg gucuguucgg aaaaggaagc cuaguaaccu gcgccaaauu cacaugcagc      420

aagaaaauga ccggaaaauc aauccagccc gaaaaccucg aauaccgcau aaugcuaucc      480

guacacggua gccaacacuc cggcaugauc gucaacgaca ucggacacga gacagacgaa      540

aaccgcgcca aaguagaagu aaccccaaac ucccccaggg ccgaagcaac acucggaggc      600

uucggauccc uaggacuaga cugcgaaccc agaaccggcc ucgauuucag cgaccuauac      660

uaccucacga ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauaccc      720

cuacccuggc acgccggcgc agacaccggc acaccccacu ggaacaacaa agaagcacuc      780

guagaguuca aagacgcaca cgcaaaacgc caaaccguag uaguccuagg cagccaagaa      840

ggcgccguac acaccgcccu ggccggagcg cuagaagcag aaauggacgg agccaaaggc      900

agacuauuca gcggccaccu aaaaugccga cuaaaaaugg acaaacucag acucaaagga      960

guaagcuaca gccuaugcac cgccguaugc accgccgcca aaguccccgc agaaacacua     1020

cacggcacag ucacaguaga aguccaauac gcaggcacgg acggccccug caaagugcca     1080

gcccaaaugg ccguagacau gcaaacucug acaccagucg gcagacucau aaccgccaac     1140

ccaguaauaa cagaaagcac ggaaaacuca aaaaugaugc uugaacucga cccgcccuuc     1200

ggagacucau acauagucau aggaguaggc gacaagaaaa ucacacacca cuggcaccgg     1260

agcggcucca cuauaggcaa agcauucgaa gcaaccguaa gaggagccaa acgaauggcc     1320

guacuagggg acacagcaug ggacuucggc ucc                                  1353


<210>  3013
<211>  1335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE CDS opt

<400>  3013
ucgcaaaaag ucauauaccu ggucaugauc cugcuuaucg cacccgcuua cuccauccgc       60

ugcaucggag ucucgaacag ggacuucguc gaaggcauga gcggaggcac auggguagac      120

guaguccuag aacacggcgg cugcgugacc gucauggcac aagacaaacc gacaguagac      180

auagaacucg ucacaaccac agucucgaac auggcagaag uuagaaguua cugcuacgaa      240

gccagcauaa gcgacauggc aagcgacagc agaugcccaa cccaagggga agcauaccug      300

gacaaacaaa gcgacaccca auacgucugc aaacgcacgc uaguagacag gggauggggc      360

aacggaugcg gacuguucgg gaaaggaagc cuagucacau gcgcgaaguu cacaugcagc      420

aagaaaauga caggaaaauc cauacaaccc gaaaaccuag aauaccgaau aaugcugagc      480

gugcacggaa gccaacacag cggcaugaua guaaacgacg aaaaccgcgc gaaaguagaa      540

guaaccccaa acagcccaag ggccgaggca acgcuaggag gauucggaag ccuagggcuc      600

gacugcgagc ccagaaccgg acucgacuuc ucggaccuau acuaccuaac gaugaacaac      660

aaacacuggc uaguacacaa agaaugguuc cacgacauac cgcuccccug gcacucggga      720

gcagacaccg aaacgccaca cuggaacaac aaagaagcgc uaguagaguu caaagacgcc      780

cacgccaagc gacagacagu agucguacuc gggagccagg aaggagccgu acacacagcc      840

cucgccggag cccucgaagc agagauggac ggagccaaag gccgacuauc guccggccac      900

cuaaaaugcc gccugaaaau ggacaaacuc aggcuaaaag gcgugagcua cagccugugc      960

accgccgcau ucacauucac aaaagugccc gcagaaacac uccauggaac agucacaguc     1020

gaaguccaau acgcaggaag ggacgggccc ugcaaaguac ccgcgcagau ggcaguagac     1080

augcaaacgc uaacccccgu cggcagacuc auaacagcga acccggucau aaccgaaagc     1140

acagaaaaca gcaaaaugau gcuagaauua gaucccccau ucggggacuc auacaucguc     1200

auaggcguag gcgacaagaa aauaacacac cacuggcaca gaucaggcuc cauaauaggc     1260

aaagccuucg aagcaacagu gcgaggagcc aaacgcaugg caguacuagg agacaccgcc     1320

ugggacuucg gcuca                                                      1335


<210>  3014
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE CDS opt

<400>  3014
ucacaaaagg uaauauaccu agucaugauc guacucauag ucccggcaua cagcauaagc       60

uguaucggag uaucaaaccg agaccuggua gagggcauga gcgggggcac auggguggac      120

gugguacugg aacauggcgg augcguaacc gaaauggcgc aagacaaacc gaccguggac      180

auagagcuag uaacaaugac cgucucgaac auggcagaag ucagaagcua cugcuacgaa      240

gcaagccuga gcgacauggc aagcgcaagu aggugcccga cccaaggaga accaucacua      300

gacaaacaaa gcgacaccca gagcguaugc aagagaacgc uaggggacag aggcugggga      360

aacggcugcg gaaucuucgg aaaaggaucc cuagucacgu gcuccaaauu uacaugcugc      420

aagaaaaugc cagggaaaag cauccagcca gagaaccucg aauauagaau aaugcuaccc      480

gugcacggca gccagcacuc aggaaugauc gugaacgaca ucggacacga gaccgacgaa      540

aaccgcgcaa aaguggaagu aaccccaaac ucacccagag cagaagccac gcucggagga      600

uucggaagcc ucgggcucga cugcgaaccc cgaaccgggc ucgacuucuc agacuuauac      660

uaccuaacaa ugaacaacaa gcacuggcuc guacacaaag agugguucca cgacauaccc      720

cugccauggc acgcgggagc agacacaggc acgccgcauu ggaacaacaa ggaggcacuc      780

guagaguuca aagacgcgca cgcaaagcga caaacagucg uagugcucgg aagccaagaa      840

ggggccgucc acacagccuu agccggggcc cucgaagcag aaauggacgg ggcaaagggc      900

cggcucuuua gcggccaccu aaaaugccgc cuaaaaaugg acaagcuaag acuaaagggc      960

guaagcuaca gccugugcac agccgcauuc acauucacaa aaguacccgc agaaacgcuc     1020

cacggcacag ucacagucga gguccagagc gcgggaacgg acggacccug caaaguacca     1080

gcgcaaaugg ccguagacau gcaaacacuc acccccgugg gaaggcuaau aacagcaaac     1140

ccgguaauaa ccgaaagcac agaaaacagc aagaugaugc ucgaguuaga cccaccauuc     1200

ggggacucau acauaguuau aggcguaggc gacaagaaaa ucacgcacca uuggcacaga     1260

uccggaagua ccaucggcaa agccuucgaa gcaaccgugc gcggggccaa gagaauggca     1320

gugcucggag auaccgcaug ggacuucggc uca                                  1353


<210>  3015
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE CDS opt

<400>  3015
cgacaaaaag ucauuuaccu aguaaugaua cuccuaauag ccccagccua cagcauuaga       60

ugcaucgggg uaucaaacag ggacuucgua gaaggaauga gcggcggaac cuggguagac      120

gucguccucg aacacggcgg cugcguaacc guaauggcac aagacaaacc gaccguagac      180

auagaacucg ucaccaccac agucucaaac auggcagaag uccggagcua cugcuacgag      240

gcuucuauaa gcgacauggc aagcgacagc agaugcccaa cccaaggcga agcauaccua      300

gacaaacaaa gcgacaccca guacguaugc aaaagaaccc ucguagaccg aggauggggc      360

aacggcugcg gccuguucgg aaaaggaagc cugguaacau gcgcaaaauu ugccugcagc      420

aaaaagauga cagggaaauc cauccaaccc gaaaaccuag aauacagaau aaugcucagc      480

guacauggaa gccaacauag cggcaugauc gucaacgaca caggacacga gacagacgaa      540

aaccgcgcaa aaguagaaau caccccaaac ucacccaggg ccgaggcaac acucggcgga      600

uucggcagcc ucggccucga cugcgaaccc agaaccggcc ucgacuucuc agaccuguac      660

uaucuaacca ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauccca      720

cugcccuggc acgccggcgc cgacacagga acaccacacu ggaacaacaa agaagcccua      780

gucgaguuca aagacgcaca cgcaaaacga caaacagucg ucguccucgg cagccaagaa      840

ggagcagucc acacggcccu ggcaggagcc cucgaagcag aaauggacgg agccaaaggc      900

aggcucucau caggacaccu aaaaugccgc cuuaaaaugg acaaacuccg acugaaagga      960

guauccuaca gccucugcac agccgcauuc accuucacaa aaauaccagc ggaaacacug     1020

cacggcacag ucaccgucga aguccaguac gcaggcaccg acggccccug caaaguacca     1080

gcacaaaugg cgguggacau gcaaacccua accccaguag gccgacuaau aaccgcgaac     1140

ccagucauaa ccgagagcac cgaaaacagu aaaaugaugc ucgagcucga cccaccauuc     1200

ggagacagcu acauaguaau aggcgucggu gaaaagaaaa ucacacacca cuggcaccgc     1260

ucgggcucaa ccaucggcaa agccuucgaa gcaacuguac gcggagccaa acgcauggca     1320

guacuaggag acacagcaug ggacuucggc uca                                  1353


<210>  3016
<211>  1353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE CDS opt

<400>  3016
agccaaagag ucaucuaccu gguaaugauc cuacuaauag ccccagccua cagcauccga       60

ugcaucgggg ucucgaacag agacuucguc gaaggcauga gcggaggcac auggguagac      120

guagugcugg aacacggcgg augcguaacc guaauggcac aagacaaacc aaccguagac      180

auagagcuag ucacaacaac agucucaaac auggccgaag ucaggaguua cugcuacgaa      240

gcaagcauaa gcgacauggc cagcgacagc cgaugcccaa cccaaggcga agcauaccua      300

gacaaacaaa gcgacaccca auacgugugc aaacgcacgc uagucgacag aggauggggc      360

aacgggugcg gccucuucgg gaaaggaagc cuagucacgu gcgcaaaauu cacaugcagc      420

aagaagauga ccggaaaauc aauccaaccc gaaaaccuag aauaccgcau aaugcugagc      480

guccacggaa gccaacacuc aggcaugauc gucaacgaca ucggacacga gaccgacgaa      540

aaccgagcga aaguggaagu aaccccaaac ucaccaaggg ccgaggccac ccucggaggc      600

uucggaucgc ucgggcuaga cugcgaaccc agaaccggac ucgacuucuc agaccucuac      660

uaccucacga ugaacaacaa acauuggcuc gugcacaaag aaugguucca cgauauccca      720

cugcccuggc acgcaggcgc ggacacagga acaccccacu ggaacaauaa ggaagcacuu      780

guagaguuca aagacgccca cgccaaacgc cagaccguag ucguacuagg aagccaagaa      840

ggagccgugc acaccgcccu ggcaggagca cuggaagcag aaauggacgg agccaaaggc      900

cgacucuuca gcggacacuu gaaaugccgc auaaaaaugg acaaacucag gcucaaaggc      960

gugagcuaca gccuaugcac ugccgcauuc acauucucca agguaccagc agaaacacuc     1020

cacggcaccg ucacgguaga aguccaauac gcaggccgag acgggccaug caaagugccc     1080

gcacaaaugg ccguagacau gcaaacgcuc acacccguag gcaggcucau aacagcgaac     1140

ccaaucauaa cagaaaguac agaaaacagc agaaugaugc uagaacucga cccaccguuc     1200

ggggacuccu acauagucau cggcgugggc gacaagaaaa uaacacacca cuggcauaga     1260

ucgggcucaa ccauaggcaa agccuucgaa gcgacagugc gaggagcaaa aagaauggcc     1320

guacuaggag acacagcaug ggauuucggc ucg                                  1353


<210>  3017
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4803/R4833 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 1 wild type

<400>  3017
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3018
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4815/R4837 ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA 
       Design 1 wild type

<400>  3018
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacgagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaaucuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3019
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4819/R4841 ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 
       1 wild type

<400>  3019
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1080

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1140

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1200

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1260

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1320

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1380

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1440

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug     1500

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1560

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1620

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1680

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1740

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1800

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1860

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1920

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1980

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     2040

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2100

gcuguuucug cugacguggg gugcucagug gacuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3020
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4867 ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 1 
       wild type

<400>  3020
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3021
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4879/R5080 ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA 
       Design 1 wild type

<400>  3021
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3022
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 1 wild 
       type

<400>  3022
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3023
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 1 wild type

<400>  3023
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3024
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4869/R5114 ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA 
       Design 1 wild type

<400>  3024
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3025
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 wild
       type

<400>  3025
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3026
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 wild 
       type

<400>  3026
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3027
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 wild type

<400>  3027
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      540

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      600

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      660

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      720

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag      780

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc      840

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca      900

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug      960

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1020

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1080

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1140

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1200

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1260

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1320

auggcagucc ugggggauac agccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3028
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 1 wild 
       type

<400>  3028
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3029
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       1 wild type

<400>  3029
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuc cggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3030
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 1
       wild type

<400>  3030
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuc cggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3031
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 1 
       wild type

<400>  3031
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuc cggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3032
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 1 
       wild type

<400>  3032
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuc cggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3033
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       1 wild type

<400>  3033
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguucagg acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3034
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 1
       wild type

<400>  3034
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguucagg acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3035
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 1 
       wild type

<400>  3035
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguucagg acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3036
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 wild 
       type

<400>  3036
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3037
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 wild 
       type

<400>  3037
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3038
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 1 wild type

<400>  3038
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gaguggcagc acgcugggca aggccuuuuc aacaacuuug     1800

aagggagcuc aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga     1860

ggggucuuca acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca     1920

cucuuugggg gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug     1980

ggcgucaacg cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc     2040

guguucuuag cgaccaaugu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3039
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 wild 
       type

<400>  3039
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3040
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 wild 
       type

<400>  3040
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3041
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3041
gggagaaagc uuaccaugau aaucaaugcu aggaaggaga agaagagacg gggcgcagau       60

accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga      120

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      180

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      240

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      300

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      360

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      420

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      480

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      540

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      600

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      660

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      720

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      780

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      840

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      900

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      960

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac     1020

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1080

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1140

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1200

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1260

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1320

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1380

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1440

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1500

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1560

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1620

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1680

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1740

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1800

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1860

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1920

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1980

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     2040

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2100

uuauccacag ccgucucugc ugaugugggg ugcucggugg acuucucaaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  3042
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3042
gggagaaagc uuaccaugau caaugcuagg aaggagaaga agagacgggg cgcagauacc       60

agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu      120

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      180

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      240

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      300

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      360

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      420

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      480

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      540

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      600

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      660

acuuggguug auauugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      720

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      780

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      840

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      900

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gucuggugac augcgcuaag      960

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg     1020

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1080

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1140

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1200

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1260

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1320

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1380

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1440

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1500

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1560

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1620

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1680

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1740

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1800

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1860

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1920

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1980

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     2040

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2100

uccacagccg ucucugcuga uguggggugc ucgguggacu ucucaaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  3043
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3043
gggagaaagc uuaccaugaa ugcuaggaag gagaagaaga gacggggcgc agauaccagu       60

gucggaauug uuggccuccu gcugaccaca gcuauggcag cggaggucac uagacguggg      120

agugcauacu auauguacuu ggacagaaac gaugcugggg aggccauauc uuuuccaacc      180

acauugggga ugaauaagug uuauauacag aucauggauc uuggacacau gugugaugcc      240

accaugagcu augaaugccc uaugcuggau gagggggugg aaccagauga cgucgauugu      300

uggugcaaca cgacgucaac uuggguugug uacggaaccu gccaucacaa aaaaggugaa      360

gcacggagau cuagaagagc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg      420

cggucgcaaa ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau      480

uggauauuca ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga      540

agcucaacga gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac      600

agcaucaggu gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu      660

uggguugaua uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg      720

acugucgaca uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac      780

ugcuaugagg caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa      840

gccuaccuug acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga      900

ggcuggggaa auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu      960

gcaugcucca agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua     1020

augcugucag uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa     1080

acugaugaga auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc     1140

cugggggguu uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca     1200

gauuuguauu acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac     1260

gacauuccau uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa     1320

gaagcacugg uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg     1380

agucaagaag gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu     1440

gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga     1500

uugaagggcg ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu     1560

gaaacacugc acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc     1620

aagguuccag cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua     1680

accgcuaacc ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau     1740

ccaccauuug gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac     1800

uggcacagga guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag     1860

agaauggcag ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac     1920

ucauugggca agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga     1980

auguccuggu ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca     2040

aagaauggau cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc     2100

acagccgucu cugcugaugu ggggugcucg guggacuucu caaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3044
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3044
gggagaaagc uuaccauggc uaggaaggag aagaagagac ggggcgcaga uaccaguguc       60

ggaauuguug gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu      120

gcauacuaua uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca      180

uuggggauga auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc      240

augagcuaug aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg      300

ugcaacacga cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca      360

cggagaucua gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      420

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      480

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      540

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      600

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      660

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      720

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      780

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      840

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      900

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      960

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug     1020

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu     1080

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug     1140

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau     1200

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac     1260

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1320

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1380

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1440

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1500

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1560

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1620

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1680

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1740

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1800

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1860

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1920

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1980

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     2040

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     2100

gccgucucug cugauguggg gugcucggug gacuucucaa agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3045
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3045
gggagaaagc uuaccaugag gaaggagaag aagagacggg gcgcagauac cagugucgga       60

auuguuggcc uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca      120

uacuauaugu acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug      180

gggaugaaua aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug      240

agcuaugaau gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc      300

aacacgacgu caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg      360

agaucuagaa gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      420

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      480

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      540

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      600

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      660

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      720

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      780

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      840

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      900

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      960

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug     1020

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau     1080

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg     1140

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug     1200

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu     1260

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1320

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1380

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1440

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1500

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1560

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1620

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1680

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1740

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1800

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1860

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1920

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1980

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     2040

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     2100

gucucugcug auguggggug cucgguggac uucucaaagu gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  3046
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3046
gggagaaagc uuaccaugaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3047
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3047
gggagaaagc uuaccaugga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugaugugg ggugcucggu ggacuucuca aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  3048
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3048
gggagaaagc uuaccaugaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gauguggggu gcucggugga cuucucaaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  3049
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3049
gggagaaagc uuaccaugaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100

guggggugcu cgguggacuu cucaaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3050
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3050
gggagaaagc uuaccaugag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugaugug     2100

gggugcucgg uggacuucuc aaagugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3051
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3051
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcucggugg acuucucaaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3052
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3052
gggagaaagc uuaccauggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga uguggggugc     2100

ucgguggacu ucucaaagug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3053
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3053
gggagaaagc uuaccauggc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcucg     2100

guggacuucu caaagugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3054
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3054
gggagaaagc uuaccaugga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     2100

gacuucucaa agugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3055
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3055
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cucgguggac     2100

uucucaaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3056
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3056
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggccg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cucgguggac     2100

uucucaaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3057
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3057
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcggc cgucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcucggugg acuucucaaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3058
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3058
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg guggagugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3059
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3059
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg gugccgugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3060
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3060
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3061
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3061
gggagaaagc uuaccaugau aaucaaugcu aggaaggaga agaagagacg gggcgcagau       60

accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga      120

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      180

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      240

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      300

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      360

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      420

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      480

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      540

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      600

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      660

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      720

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      780

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      840

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      900

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      960

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac     1020

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1080

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1140

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1200

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1260

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1320

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1380

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1440

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1500

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1560

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1620

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1680

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1740

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1800

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1860

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1920

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1980

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     2040

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2100

uuauccacag ccgucucugc ugaugugggg ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  3062
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3062
gggagaaagc uuaccaugau caaugcuagg aaggagaaga agagacgggg cgcagauacc       60

agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu      120

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      180

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      240

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      300

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      360

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      420

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      480

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      540

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      600

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      660

acuuggguug auauugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      720

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      780

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      840

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      900

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gucuggugac augcgcuaag      960

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg     1020

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1080

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1140

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1200

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1260

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1320

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1380

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1440

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1500

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1560

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1620

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1680

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1740

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1800

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1860

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1920

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1980

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     2040

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2100

uccacagccg ucucugcuga uguggggugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  3063
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3063
gggagaaagc uuaccaugaa ugcuaggaag gagaagaaga gacggggcgc agauaccagu       60

gucggaauug uuggccuccu gcugaccaca gcuauggcag cggaggucac uagacguggg      120

agugcauacu auauguacuu ggacagaaac gaugcugggg aggccauauc uuuuccaacc      180

acauugggga ugaauaagug uuauauacag aucauggauc uuggacacau gugugaugcc      240

accaugagcu augaaugccc uaugcuggau gagggggugg aaccagauga cgucgauugu      300

uggugcaaca cgacgucaac uuggguugug uacggaaccu gccaucacaa aaaaggugaa      360

gcacggagau cuagaagagc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg      420

cggucgcaaa ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau      480

uggauauuca ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga      540

agcucaacga gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac      600

agcaucaggu gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu      660

uggguugaua uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg      720

acugucgaca uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac      780

ugcuaugagg caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa      840

gccuaccuug acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga      900

ggcuggggaa auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu      960

gcaugcucca agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua     1020

augcugucag uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa     1080

acugaugaga auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc     1140

cugggggguu uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca     1200

gauuuguauu acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac     1260

gacauuccau uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa     1320

gaagcacugg uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg     1380

agucaagaag gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu     1440

gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga     1500

uugaagggcg ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu     1560

gaaacacugc acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc     1620

aagguuccag cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua     1680

accgcuaacc ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau     1740

ccaccauuug gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac     1800

uggcacagga guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag     1860

agaauggcag ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac     1920

ucauugggca agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga     1980

auguccuggu ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca     2040

aagaauggau cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc     2100

acagccgucu cugcugaugu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3064
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3064
gggagaaagc uuaccauggc uaggaaggag aagaagagac ggggcgcaga uaccaguguc       60

ggaauuguug gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu      120

gcauacuaua uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca      180

uuggggauga auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc      240

augagcuaug aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg      300

ugcaacacga cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca      360

cggagaucua gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      420

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      480

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      540

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      600

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      660

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      720

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      780

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      840

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      900

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      960

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug     1020

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu     1080

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug     1140

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau     1200

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac     1260

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1320

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1380

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1440

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1500

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1560

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1620

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1680

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1740

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1800

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1860

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1920

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1980

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     2040

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     2100

gccgucucug cugauguggg gugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3065
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3065
gggagaaagc uuaccaugag gaaggagaag aagagacggg gcgcagauac cagugucgga       60

auuguuggcc uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca      120

uacuauaugu acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug      180

gggaugaaua aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug      240

agcuaugaau gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc      300

aacacgacgu caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg      360

agaucuagaa gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      420

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      480

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      540

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      600

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      660

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      720

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      780

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      840

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      900

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      960

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug     1020

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau     1080

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg     1140

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug     1200

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu     1260

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1320

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1380

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1440

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1500

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1560

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1620

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1680

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1740

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1800

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1860

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1920

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1980

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     2040

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     2100

gucucugcug auguggggug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3066
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3066
gggagaaagc uuaccaugaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3067
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3067
gggagaaagc uuaccaugga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugaugugg ggugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3068
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3068
gggagaaagc uuaccaugaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gauguggggu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3069
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3069
gggagaaagc uuaccaugaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100

guggggugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3070
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3070
gggagaaagc uuaccaugag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugaugug     2100

gggugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3071
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3071
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3072
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3072
gggagaaagc uuaccauggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga uguggggugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3073
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3073
gggagaaagc uuaccauggc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3074
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3074
gggagaaagc uuaccaugga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3075
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3075
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3076
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3076
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggccg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3077
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3077
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcggc cgucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3078
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3078
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg guggagugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3079
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3079
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg gugccgugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3080
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3080
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugauuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3081
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3081
gggagaaagc uuaccaugau aaucaaugcu aggaaggaga agaagagacg gggcgcagau       60

accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga      120

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      180

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      240

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      300

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      360

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      420

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      480

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      540

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      600

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      660

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      720

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      780

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      840

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      900

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      960

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac     1020

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1080

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1140

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1200

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1260

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1320

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1380

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1440

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1500

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1560

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1620

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1680

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1740

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1800

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1860

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1920

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1980

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     2040

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2100

uuauccacag ccgucucugc ugauugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3082
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3082
gggagaaagc uuaccaugau caaugcuagg aaggagaaga agagacgggg cgcagauacc       60

agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu      120

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      180

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      240

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      300

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      360

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      420

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      480

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      540

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      600

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      660

acuuggguug auauugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      720

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      780

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      840

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      900

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gucuggugac augcgcuaag      960

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg     1020

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1080

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1140

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1200

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1260

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1320

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1380

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1440

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1500

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1560

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1620

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1680

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1740

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1800

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1860

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1920

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1980

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     2040

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2100

uccacagccg ucucugcuga uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3083
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3083
gggagaaagc uuaccaugaa ugcuaggaag gagaagaaga gacggggcgc agauaccagu       60

gucggaauug uuggccuccu gcugaccaca gcuauggcag cggaggucac uagacguggg      120

agugcauacu auauguacuu ggacagaaac gaugcugggg aggccauauc uuuuccaacc      180

acauugggga ugaauaagug uuauauacag aucauggauc uuggacacau gugugaugcc      240

accaugagcu augaaugccc uaugcuggau gagggggugg aaccagauga cgucgauugu      300

uggugcaaca cgacgucaac uuggguugug uacggaaccu gccaucacaa aaaaggugaa      360

gcacggagau cuagaagagc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg      420

cggucgcaaa ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau      480

uggauauuca ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga      540

agcucaacga gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac      600

agcaucaggu gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu      660

uggguugaua uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg      720

acugucgaca uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac      780

ugcuaugagg caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa      840

gccuaccuug acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga      900

ggcuggggaa auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu      960

gcaugcucca agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua     1020

augcugucag uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa     1080

acugaugaga auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc     1140

cugggggguu uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca     1200

gauuuguauu acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac     1260

gacauuccau uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa     1320

gaagcacugg uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg     1380

agucaagaag gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu     1440

gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga     1500

uugaagggcg ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu     1560

gaaacacugc acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc     1620

aagguuccag cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua     1680

accgcuaacc ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau     1740

ccaccauuug gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac     1800

uggcacagga guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag     1860

agaauggcag ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac     1920

ucauugggca agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga     1980

auguccuggu ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca     2040

aagaauggau cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc     2100

acagccgucu cugcugauug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3084
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3084
gggagaaagc uuaccauggc uaggaaggag aagaagagac ggggcgcaga uaccaguguc       60

ggaauuguug gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu      120

gcauacuaua uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca      180

uuggggauga auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc      240

augagcuaug aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg      300

ugcaacacga cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca      360

cggagaucua gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      420

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      480

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      540

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      600

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      660

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      720

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      780

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      840

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      900

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      960

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug     1020

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu     1080

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug     1140

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau     1200

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac     1260

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1320

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1380

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1440

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1500

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1560

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1620

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1680

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1740

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1800

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1860

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1920

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1980

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     2040

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     2100

gccgucucug cugauugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3085
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3085
gggagaaagc uuaccaugag gaaggagaag aagagacggg gcgcagauac cagugucgga       60

auuguuggcc uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca      120

uacuauaugu acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug      180

gggaugaaua aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug      240

agcuaugaau gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc      300

aacacgacgu caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg      360

agaucuagaa gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      420

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      480

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      540

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      600

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      660

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      720

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      780

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      840

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      900

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      960

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug     1020

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau     1080

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg     1140

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug     1200

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu     1260

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1320

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1380

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1440

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1500

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1560

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1620

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1680

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1740

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1800

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1860

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1920

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1980

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     2040

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     2100

gucucugcug auugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3086
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3086
gggagaaagc uuaccaugaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugauu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3087
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3087
gggagaaagc uuaccaugga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugauugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3088
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3088
gggagaaagc uuaccaugaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gauugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3089
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3089
gggagaaagc uuaccaugaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3090
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3090
gggagaaagc uuaccaugag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugauuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3091
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3091
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugauugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3092
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3092
gggagaaagc uuaccauggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3093
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3093
gggagaaagc uuaccauggc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugauug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3094
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3094
gggagaaagc uuaccaugga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  3095
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3095
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3096
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3096
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggccg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3097
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3097
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcggc cgucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugauugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3098
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3098
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg guggagugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3099
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3099
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg gugccgugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3100
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3100
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcuugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3101
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3101
gggagaaagc uuaccaugau caaugcuagg aaggagaaga agagacgggg cgcagauacc       60

agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu      120

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      180

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      240

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      300

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      360

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      420

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      480

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      540

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      600

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      660

acuuggguug auauugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      720

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      780

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      840

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      900

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gucuggugac augcgcuaag      960

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg     1020

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1080

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1140

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1200

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1260

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1320

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1380

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1440

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1500

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1560

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1620

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1680

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1740

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1800

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1860

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1920

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1980

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     2040

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2100

uccacagccg ucucugcuug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3102
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3102
gggagaaagc uuaccaugau aaucaaugcu aggaaggaga agaagagacg gggcgcagau       60

accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga      120

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      180

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      240

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      300

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      360

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      420

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      480

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      540

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      600

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      660

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      720

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      780

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      840

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      900

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      960

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac     1020

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1080

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1140

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1200

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1260

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1320

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1380

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1440

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1500

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1560

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1620

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1680

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1740

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1800

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1860

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1920

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1980

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     2040

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2100

uuauccacag ccgucucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3103
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3103
gggagaaagc uuaccaugaa ugcuaggaag gagaagaaga gacggggcgc agauaccagu       60

gucggaauug uuggccuccu gcugaccaca gcuauggcag cggaggucac uagacguggg      120

agugcauacu auauguacuu ggacagaaac gaugcugggg aggccauauc uuuuccaacc      180

acauugggga ugaauaagug uuauauacag aucauggauc uuggacacau gugugaugcc      240

accaugagcu augaaugccc uaugcuggau gagggggugg aaccagauga cgucgauugu      300

uggugcaaca cgacgucaac uuggguugug uacggaaccu gccaucacaa aaaaggugaa      360

gcacggagau cuagaagagc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg      420

cggucgcaaa ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau      480

uggauauuca ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga      540

agcucaacga gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac      600

agcaucaggu gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu      660

uggguugaua uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg      720

acugucgaca uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac      780

ugcuaugagg caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa      840

gccuaccuug acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga      900

ggcuggggaa auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu      960

gcaugcucca agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua     1020

augcugucag uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa     1080

acugaugaga auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc     1140

cugggggguu uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca     1200

gauuuguauu acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac     1260

gacauuccau uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa     1320

gaagcacugg uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg     1380

agucaagaag gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu     1440

gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga     1500

uugaagggcg ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu     1560

gaaacacugc acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc     1620

aagguuccag cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua     1680

accgcuaacc ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau     1740

ccaccauuug gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac     1800

uggcacagga guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag     1860

agaauggcag ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac     1920

ucauugggca agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga     1980

auguccuggu ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca     2040

aagaauggau cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc     2100

acagccgucu cugcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3104
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3104
gggagaaagc uuaccauggc uaggaaggag aagaagagac ggggcgcaga uaccaguguc       60

ggaauuguug gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu      120

gcauacuaua uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca      180

uuggggauga auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc      240

augagcuaug aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg      300

ugcaacacga cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca      360

cggagaucua gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      420

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      480

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      540

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      600

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      660

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      720

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      780

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      840

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      900

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      960

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug     1020

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu     1080

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug     1140

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau     1200

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac     1260

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1320

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1380

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1440

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1500

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1560

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1620

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1680

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1740

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1800

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1860

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1920

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1980

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     2040

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     2100

gccgucucug cuugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3105
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3105
gggagaaagc uuaccaugag gaaggagaag aagagacggg gcgcagauac cagugucgga       60

auuguuggcc uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca      120

uacuauaugu acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug      180

gggaugaaua aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug      240

agcuaugaau gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc      300

aacacgacgu caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg      360

agaucuagaa gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      420

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      480

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      540

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      600

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      660

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      720

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      780

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      840

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      900

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      960

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug     1020

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau     1080

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg     1140

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug     1200

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu     1260

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1320

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1380

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1440

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1500

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1560

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1620

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1680

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1740

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1800

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1860

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1920

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1980

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     2040

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     2100

gucucugcuu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3106
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3106
gggagaaagc uuaccaugaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3107
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3107
gggagaaagc uuaccaugga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcuugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3108
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3108
gggagaaagc uuaccaugaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3109
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3109
gggagaaagc uuaccaugaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3110
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3110
gggagaaagc uuaccaugag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcuugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3111
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3111
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3112
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3112
gggagaaagc uuaccauggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3113
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3113
gggagaaagc uuaccauggc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcuugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  3114
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3114
gggagaaagc uuaccaugga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cuugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3115
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 wild
       type

<400>  3115
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3116
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3116
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggccg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3117
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3117
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcggc cgucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3118
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3118
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg guggagugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3119
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 wild type

<400>  3119
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg gugccgugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3120
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 1 wild type

<400>  3120
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3121
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 1 wild type

<400>  3121
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaaucuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3122
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 1 wild type

<400>  3122
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1080

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1140

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1200

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1260

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1320

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1380

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1440

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug     1500

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1560

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1620

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1680

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1740

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1800

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1860

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1920

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1980

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     2040

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2100

gcuguuucug cugacguggg gugcucagug gacuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3123
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 1 wild type

<400>  3123
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3124
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 1 wild type

<400>  3124
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugu caaugauaca     1080

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu     1200

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1380

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1440

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1560

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3125
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 1 wild type

<400>  3125
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggcgca       60

gacaccagca ucggaaucgu uggccuccug uugacuacag ccauggcagc agagaucacu      120

agacguggga gugcauacua cauguacuug gauaggagcg augcagggaa ggccauuucu      180

uucgcuacca cauugggggu gaacaaaugc caugugcaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugagcua ugaaugcccu augcuggacg agggggugga accagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacggcgauc uagaagagcc gucacgcucc caucucacuc cacaaggaaa      420

uugcaaacgc ggucgcagac uuggcuagaa ucaagagaau acacaaagca ccugaucaag      480

guugaaaauu ggauauucag gaacccuggu uuuacgcuag uggcugucgc caucgccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauaggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugacgu uguccuggaa cauggaggcu gcgucaccgu gauggcacag      720

gacaagccaa caguugacau agagcugguc acaacaacgg uuaguaacau ggccgaggug      780

agauccuauu guuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cucuuuggca aagggaguuu ggugacaugu      960

gcuaaguuca cgugcuccaa gaagaugacu gggaagagca uucagccgga gaaccuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcacagug ggaugauugu uaaugaugaa     1080

aacagagcga aggucgaggu uacgcccaau ucaccaagag cagaagcaac ccugggaggc     1140

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau     1200

uaccuaacca ugaauaacaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uugcccuggc augcuggggc agacacugga acuccacauu ggaacaacaa ggaggcauua     1320

guggaauuca aggacgccca cgccaagagg caaaccgucg ugguuuuggg gagccaggaa     1380

ggagccgucc acacggcucu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aggcuauucu cuggccacuu gaaaugucgc uuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac cgcggcauuc acauucacca aggucccggc ugaaacacua     1560

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1620

gcccagaugg cgguggacau gcagaccuug accccagucg gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gauaagaaaa ucacccauca cuggcauagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggcgcuaa gagaauggca     1860

guccuggggg acacagcuug ggacuuugga ucagucggag guguguuuaa cucauugggc     1920

aagggcauuc aucagauuuu uggagcagcu uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacucugcug gugugguuag gucugaacac aaagaauggg     2040

ucuaucuccc ucacaugcuu agcccugggg ggagugauga ucuuccucuc cacggcuguu     2100

ucugcugacg uggggugcuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3126
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 1 wild type

<400>  3126
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucgcaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugu caaugauaca     1080

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggucuu     1200

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1380

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1440

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1560

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3127
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 1 wild type

<400>  3127
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugacugu caaugauaua     1080

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu     1200

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag acacuggaac uccacacugg     1320

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1380

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1440

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1560

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3128
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 1 wild type

<400>  3128
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaccggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1080

gacgaaaaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1140

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1200

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1260

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1320

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1380

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1440

aagggaaagc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug     1500

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1560

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1620

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1680

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1740

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1800

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1860

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1920

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1980

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     2040

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2100

gcuguuucug cugacguggg gugcucagug gacuucucaa agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3129
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 1 wild type

<400>  3129
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcag gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3130
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 1 wild type

<400>  3130
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaugcuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3131
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 1 wild type

<400>  3131
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuagaaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc cucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3132
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 1 wild type

<400>  3132
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccua cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu gguacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3133
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 1 wild type

<400>  3133
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggagaua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu ggaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcugcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3134
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 1 wild type

<400>  3134
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguucuagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3135
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 1 wild type

<400>  3135
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu acgcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccagcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguc acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcaguacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3136
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 1 wild type

<400>  3136
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu agaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacugau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacuccuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagucgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3137
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 1 wild type

<400>  3137
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucagcu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggccgcaaac cugguuggaa ucaagagaau acacaaagca uuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacaag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3138
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 1 wild type

<400>  3138
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa guucaacgag caaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggga cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3139
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 1 wild type

<400>  3139
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgcg ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu agaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacugau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacuccuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3140
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 1 wild type

<400>  3140
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaagcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca cuaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaauaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacacgc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3141
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 1 wild type

<400>  3141
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuaaug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac uugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacuccaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccagga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3142
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 1 wild type

<400>  3142
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaaacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcc gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggu uucgcuuuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa uguugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3143
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 1 wild type

<400>  3143
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc caccaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca uuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacaag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gccucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cugacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3144
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 1 wild type

<400>  3144
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuaaug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaacu ggauguucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca aggucccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3145
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 1 wild type

<400>  3145
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugagugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauacucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauauuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacucagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gaaucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3146
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 1 wild type

<400>  3146
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu ucaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aacaauauca gacauagcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugccgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3147
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 1 wild type

<400>  3147
gggagaaagc uuaccaugag aauaaucaau gccaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaaucgu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggggguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagcuua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga cucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3148
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 1 wild type

<400>  3148
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc caccaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cugacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accgagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagu uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3149
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 1 wild type

<400>  3149
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaaacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gauagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcc gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

aucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga uaagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ccagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3150
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 1 wild type

<400>  3150
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcgcuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccu ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcauug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucaucggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3151
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 1 wild type

<400>  3151
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug uaaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa aaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuuaggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuuccuuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3152
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 1 wild type

<400>  3152
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgccugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaaa gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3153
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 1 wild type

<400>  3153
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagcuua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gaagcacaug cuaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3154
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 1 wild type

<400>  3154
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacc      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguug      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc aggggcagau     1620

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3155
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 1 wild type

<400>  3155
gggagaaagc uuaccaugag aauaaucaau gcugggaagg agaagaagag acgaggcgca       60

gauacuaaug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac uugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac guucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

aggcugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacuccaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccagga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3156
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 1 wild type

<400>  3156
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaaacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaaag augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcc gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggu uucgcuuuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa uguugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcauaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3157
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 1 wild type

<400>  3157
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc agaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacaugguuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuucucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg agugcugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3158
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 1 wild type

<400>  3158
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuaaug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacau gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugaccugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguccac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag ugguagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaacggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3159
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 1 wild type

<400>  3159
gggagaaagc uuaccaugag aauaaucaau gccaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugcgaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaauac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccgccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaacu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3160
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 1 wild type

<400>  3160
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaaacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcc gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggu uucgcuuuag cagcagcugc caucgcgugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

cuggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa uguugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ccagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3161
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 1 wild type

<400>  3161
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc cauagcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaauc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucagu augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaauccua ggacuggauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3162
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 1 wild type

<400>  3162
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaagucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cauuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca ugggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3163
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 1 wild type

<400>  3163
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uucccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacagagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguu auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggaggcu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacccaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuggauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagau acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3164
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 1 wild type

<400>  3164
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augccgggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaagcgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cuguugacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagcuu ggugacaugc      960

gcuaaguuug caugcuccaa gaagaugacc gggaagagca uccagccaga gaauuuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa cagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaagag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggu     1920

gcucuuaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3165
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 1 wild type

<400>  3165
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggag ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gauacccaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggaa aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ccauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggac     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcaguacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggc     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3166
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 1 wild type

<400>  3166
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcaca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc cagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc aguaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aacaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac acuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaggcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

guucuuaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc cgcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3167
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 1 wild type

<400>  3167
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc cuucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgc aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugcucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggc ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag ccaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacagugga cgggacaguc acaguggagg gacaguacgg agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc agacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuauug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggga cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc agguggugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3168
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 1 wild type

<400>  3168
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcaca       60

gauacuagug ucggaauugu uggccuccug cugaccacag ccauggcagc ggaagucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguugg cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucuccaag     1560

aucccggcug aaacacugca cgggacaguc acagaggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc gguaaucacu gaaagcacag agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3169
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 1 wild type

<400>  3169
gggagaaagc uuaccaugag aauaaucaau gccaggaagg agaagaagag acgaggcaca       60

gacacuagug ucggaauugu uggccuccug cugaccacag ccauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccacuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cucuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcu      600

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggcgaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gccaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccgaauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaauucaag gaugcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaggg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc cuguguaccg cagcguucac auucacuaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3170
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 1 wild type

<400>  3170
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcaca       60

gauacuagug ucggaauugu uggccuccug cugaccacag ccauggcagu ggaggucacu      120

agacguggga augcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca caauggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca ccugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug cuugcucuaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugauaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcau gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca gacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucacuaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacuccaa gaugaugcug     1740

gaacuggauc caccauuugg ggacucuuac auugucauag gagucgggga aaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguugggggu     1920

gcucucaacu cacugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcuggu gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3171
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 1 wild type

<400>  3171
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg aaaagaagag acgaggcaca       60

gacacuagug ucggaauugu uggccuccug cugaccacag ccauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cgcggagauc uagaagagcu gugacgcucc ccucccacuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguugg cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu cgucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcagaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gccaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccgaauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gaugcacaug ccaaaaggca aacugucgug     1380

guccuaggga gucaagaggg agcaguucac acggcucuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagac ugaagggcgu gucauacucc cuguguaccg cagcguucac auucacuaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa ggguauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu gugugcuugg ccuuaggggg aguguugauc     2100

uucuugucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3172
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 1 wild type

<400>  3172
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcaca       60

gauacuagug ucggaauugu uggccuccug cugaccacag ccauggcagu ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaacaagugu uacauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu auguuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacaucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau auacaaagca ccugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgccugg      540

cuuuugggaa guucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaaaccgg cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuauu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcu     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcau gcuggggcag acaccggaac uccacauugg     1320

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

aggcugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaucacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg auuuuggauc aguuggaggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcuggu gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu acgugcuugg ccuuaggggg aguguugauc     2100

uuuuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3173
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 1 wild type

<400>  3173
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagcg ucggaauugu uggccuccuc cugaccacag ccauggcagu agaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uacauacaaa ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu auguuggaug aggggguaga accagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu auggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca ccugauuaga      480

guugaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugu caucgcuugg      540

cuuuugggaa guucaacgag ccaaaaaguc auauaucugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gauauggcuu cggacagccg cugcccaaca      840

caaggugagg ccuaccuuga caagcaguca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu ugggagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua ccugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcau gcuggggcag acacuggaac uccacauugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug caaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggag ccaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uugugcacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcaccg agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaucacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguugggggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uucggaggaa uguccugguu cucacaaauu cucauuggaa cguugcuggu gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu acgugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccguuuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3174
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 1 wild type

<400>  3174
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acguggcgca       60

gacaccagug ucggaauugu uggccuccug cugaccacag ccauggcagu ggaggucacc      120

agacguggga gugcauacua uauguacuua gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacugggggu gaauaagugu uacauacaga ucauggaucu uggacacaug      240

ugugaugcca caaugagcua ugaaugcccu auguuggaug aggggguaga accagaugac      300

gucgauugcu ggugcaacac gacaucgacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugagg cacggagauc uagaagagcu gugacgcucc ccucucauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugaucaga      480

gucgaaaauu ggauauucag gaacccuggc uuugcguugg cagcagcugc cauugcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu guugauugcc      600

ccggcauaca guaucaggug cauaggaguc agcaauaggg auuuugugga agguauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaagccaa cuguugauau agaguugguc acaacaacgg uuagcaacau ggcggaggua      780

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau auguuugcaa aagaacguua      900

guggacagag guuggggaaa uggaugugga cucuuuggca aagggagccu ggugacaugc      960

gccaaguuug caugcuccaa gaaaaugacu gggaagagca uccagccaga gaaccuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugauugu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu gcacaaggag     1260

ugguuccaug acauuccacu accuuggcau gcuggggcag acaccggaac uccacauugg     1320

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agccguucac acggcucuug cuggagcccu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgcuu gaaaauggac     1500

aaacuuagau ugaagggcgu gucauacucc uuauguaccg cggcguucac auucaccaag     1560

aucccggcug aaacgcugca ugggacaguc acaguggagg uacaguaugc agggacagau     1620

ggacccugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc ugugaucacu gaaagcacug agaauucaaa gaugauguug     1740

gaacuugacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaagauc     1800

acccaccacu gguacaggag uggcagcacc aucggaaaag cauuugaagc cacugugaga     1860

ggcgccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc agucggaggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauaggaa cguugcuggu gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu acgugcuugg ccuuaggggg aguguugauc     2100

uuccuaucua cagccgucuc ugcugaugug ggguguucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3175
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 1 wild type

<400>  3175
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acguggcgca       60

gacaccagug ucggaauugu uggccuccug cugaccacag ccauggcagu ggaggucacc      120

agacguggga gugcauacua uauguacuua gacagaagcg augcugggaa ggccauaucu      180

uuuccaacca cacugggggu gaauaagugu uacauacaga ucauggaucu uggacacaug      240

ugugaugcca caaugagcua ugaaugcccu auguuggaug aggggguaga accagaugac      300

gucgauugcu ggugcaacac gacaucgacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugagg cacggagauc uagaagagcu gugacgcucc ccucucauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugaucaga      480

gucgaaaauu ggauauucag gaacccuggc uuugcguugg cagcagcugc cauugcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu guugauugcc      600

ccggcauaca guaucaggug cauaggaguc agcaauaggg auuuugugga agguauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaagccaa cuguugauau agaguugguc acaacaacgg uuagcaacau ggcggaggua      780

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau auguuugcaa aagaacguua      900

guggacagag guuggggaaa uggaugugga cucuuuggca aagggagccu ggugacaugc      960

gccaaguuug caugcuccaa gaaaaugacu gggaagagca uccagccaga gaaccuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugauugu uaaugacaua     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu gcacaaggag     1260

ugguuccaug acauuccacu accuuggcau gcuggggcag acaccggaac uccacauugg     1320

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agccguucac acggcucuug cuggagcccu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgcuu gaaaauggac     1500

aaacuuagau ugaagggcgu gucauacucc uuauguaccg cggcguucac auucaccaag     1560

aucccggcug aaacgcugca ugggacaguc acaguggagg uacaguaugc agggacagau     1620

ggacccugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc ugugaucacu gaaagcacug agaauucaaa gaugauguug     1740

gaacuugacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc aucggaaaag cauuugaagc cacugugaga     1860

ggcgccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc agucggaggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauaggaa cguugcuggu gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu acgugcuugg ccuuaggggg aguguugauc     2100

uuccuaucua cagccgucuc ugcugaugug ggguguucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3176
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 1 wild type

<400>  3176
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggcgca       60

gacaccagca ucggaaucgu uggccuccug uugacuacag ccauggcagc agagaucacu      120

agacguggga gugcauacua cauguacuug gauaggagcg augcagggaa ggccauuucu      180

uucgcuacca cauugggggu gaacaaaugc caugugcaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugagcua ugaaugcccu augcuggacg agggggugga accagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacggcgauc uagaagagcc gucacgcucc caucucacuc cacaaggaaa      420

uugcaaacgc ggucgcagac uuggcuagaa ucaagagaau acacaaagca ccugaucaag      480

guugaaaauu ggauauucag gaacccuggu uuuacgcuag uggcugucgc caucgccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauaggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugacgu uguccuggaa cauggaggcu gcgucaccgu gauggcacag      720

gacaagccaa caguugacau agagcugguc acaacaacgg uuaguaacau ggccgaggug      780

agauccuauu guuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cucuuuggca aagggaguuu ggugacaugu      960

gcuaaguuca cgugcuccaa gaagaugacu gggaagagca uucagccgga gaaccuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcacagug ggaugauugu uaaugacaca     1080

ggacaugaaa cugaugaaaa cagagcgaag gucgagguua cgcccaauuc accaagagca     1140

gaagcaaccc ugggaggcuu uggaagcuua ggacuugauu gugaaccaag gacaggccuu     1200

gacuuuucag aucuguauua ccuaaccaug aauaacaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gcccuggcau gcuggggcag acacuggaac uccacauugg     1320

aacaacaagg aggcauuagu ggaauucaag gacgcccacg ccaagaggca aaccgucgug     1380

guuuugggga gccaggaagg agccguccac acggcucuug cuggagcucu agaggcugag     1440

auggauggug caaagggaag gcuauucucu ggccacuuga aaugucgcuu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcaccg cggcauucac auucaccaag     1560

gucccggcug aaacacuaca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guggacaugc agaccuugac cccagucgga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaauucaaa gaugauguug     1740

gagcucgacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaaaauc     1800

acccaucacu ggcauaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggcgcuaaga gaauggcagu ccugggggac acagcuuggg acuuuggauc agucggaggu     1920

guguuuaacu cauugggcaa gggcauucau cagauuuuug gagcagcuuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cucugcuggu gugguuaggu     2040

cugaacacaa agaauggguc uaucucccuc acaugcuuag cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3177
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 1 wild type

<400>  3177
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggugca       60

gacaccagca ucggaaucau uggucuccug cugacuacag ccauggcagc ggagauuacc      120

agacguggaa gugcauacua cauguacuug gacaggagcg augcugggaa ggccaucucc      180

uuugcuacca acuugggagu uaacaaaugc cauguacaga ucauggaucu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu auguuggacg agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugaag cacggcgauc uagaagagcu gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca ccugaucaag      480

guugaaaacu ggauauuuag gaaccccggg uuugcgcucg cagcuguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg uuaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauuggaguc agcaauagag acuucgugga aggcauguca      660

ggugggaccu ggguugaugu cgucuuggaa cauggagguu gcgucaccgu uauggcacag      720

gacaagccaa caguugacau agaguugguc acgacaacgg uuaguaacau ggccgaggua      780

agauccuacu guuaugaggc aucaauauca gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga uaagcaauca gacacccaau augugugcaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cuuuuuggca aagggagccu ggugacaugu      960

gccaaguuca cguguuccaa gaagaugacu gggaagagca uucagccgga gaaucuggag     1020

uaucgaauaa ugcuaucagu gcauggcucc cagcacagcg ggaugauugu uaaugacaua     1080

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguca cgcccaauuc accaagagca     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugauu gugaaccaag gacaggccug     1200

gacuuuucag auuuguacua cuugaccaug aacaacaagc acugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag auaccggaac uccacacugg     1320

aacaacaagg aggcauuggu agaguucaaa gaugcccacg ccaagaggca aaccgucguu     1380

guucuaggga gccaggaagg agccguucac acggcucuug cuggagcucu ggaggcugag     1440

auggauggug caaaaggaag gcuguucucc ggccauuuga aaugccgcuu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcaccg cagcguucac auuuaccaag     1560

gucccagcug aaacauugca uggaacaguu acaguggagg ugcaguaugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccagucggg     1680

aggcugauaa ccgccaaccc ugugauuacu gaaagcacug agaauucaaa gaugauguug     1740

gagcucgacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaucacu ggcauaggag ugguagcacc aucggaaaag cauuugaggc cacugugaga     1860

ggugccaaga gaauggcagu ucugggggau acagccuggg acuucggauc agucgggggc     1920

guguucaacu cauugggcaa ggguguucac cagauuuuug gagcagcuuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuggu gugguugggu     2040

uugaacacaa agaauggauc caucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcugucuc ugcugacgug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3178
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 1 wild type

<400>  3178
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa ugguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuca cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugu caaugauaua     1080

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguua cgccuaauuc accaagagcg     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu     1200

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1380

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1440

auggauggug caaagggaag gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1560

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3179
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 1 wild type

<400>  3179
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggugca       60

gacaccagca ucggaaucau uggucuccug cugacuacag ccauggcagc agagauuacc      120

agacguggaa gugcauacua cauguacuug gacaggagcg augcugggaa ggccaucucc      180

uuugcuacca auuugggagu uaacaaaugc cauguacaga ucauggaucu cgggcacaug      240

ugugacgcca ccaugaguua ugaguguccu auguuggacg agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugaag cacggcgauc uagaagagcu gugacgcucc cuucucacuc uacaaggaag      420

cugcaaacgc ggucgcagac uugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcucg cagcuguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauuggaguc agcaauagag acuucgugga aggcauguca      660

ggugggaccu ggguugaugu cgucuuggaa cauggagguu gcgucaccgu aauggcacag      720

gacaagccaa caguugacau agaguugguc acgacaacgg ucaguaacau ggccgaggua      780

agauccuacu guuaugaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguaugcaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cuuuuuggca aagggagccu ggugacaugu      960

gccaaguuca cguguuccaa gaagaugacc gggaagagca uucagccgga gaaucuggag     1020

uaucgaauaa ugcuaucagu gcauggcucc cagcacagcg ggaugauugu uaaugacgaa     1080

aauagggcga aagucgaggu cacgcccaau ucaccaagag cagaagcaac cuugggaggc     1140

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uggacuuuuc agauuuguac     1200

uacuugacca ugaacaauaa gcacugguua gugcacaaag agugguuuca ugacauccca     1260

uugcccuggc augcuggggc agacaccgga acuccacauu ggaacaacaa ggaggcauug     1320

guagaguuca aagaugccca cgcuaagagg caaaccgucg uuguucuagg gagccaggaa     1380

ggagccguuc acacggcucu ugcuggagcu cuggaggcug agauggaugg ugcaaaagga     1440

agguuguucu ccggccauuu gaaaugccgc uuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac cgcagcguuc acauucacca aggucccagc ugaaacauug     1560

cauggaacag uuacagugga ggugcaguau gcagggacag auggacccug caagguccca     1620

gcucagaugg cgguggacau gcagacccug accccagucg gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gacaagaaaa uuacccacca cuggcauagg     1800

agugguagca ccaucggaaa agcauuugag gccacuguga gaggugccaa gagaauggca     1860

guucuggggg acacagccug ggacuuugga ucagucgggg gcguguuuaa cucauugggc     1920

aagggcaucc accagauuuu uggagcagcc uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacgcugcug gugugguugg guuugaacac aaagaaugga     2040

uccaucuccc ucacaugcuu ggcccugggg ggagugauga ucuuccucuc cacggcuguc     2100

ucugcugacg uggggugcuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3180
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 1 wild type

<400>  3180
gggagaaagc uuaccaugag aauuaucaau gcuaggaagg agaggaagag acguggcgcu       60

gacaccagca ucggaaucgu cggccuccug cugaccacag ccauggcagc cgagaucacu      120

agacguggga gugcauacua cauguacuug gacaggagcg augcugguaa ggccauuucu      180

uuugccacca cauugggggu gaacaaaugc cauguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccc augcuagacg agggagugga gccagaugac      300

gucgauugcu ggugcaacac gacaucgacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacgacgauc cagaagagcc gugacgcuuc cuucucacuc cacgaggaag      420

cugcaaacgc gaucgcagac uuggcuagag ucaagagaau acacaaagca ccugaucaag      480

guugaaaauu ggauauucag gaaccccggg uuugcgcuag uggcuguagc uauagccugg      540

cuccugggaa gcucgacgag ccaaaaaguu auauacuugg ucaugauauu guugauugcc      600

ccggcauaca gcauuaggug cauaggaguu agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggggguu gcgucaccgu gauggcacag      720

gacaagccaa caguugauau cgaguugguc acgacaacgg uuagcaacau ggccgaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg uuguccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa gagaacauug      900

guggauagag guuggggaaa ugggugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cgugcuccaa gaaaaugaca ggcaagagca uccagccgga gaacuuggag     1020

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacaca     1080

ggacaugaaa cugacgaaaa cagagcaaaa gucgagguca cacccaauuc accaagagca     1140

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu     1200

gacuucucag aucuguauua cuugaccaug aacaauaagc auugguuggu gcacaaggag     1260

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg     1320

aacaacaaag aggcauuggu ggaguucaag gacgcccacg ccaagaggca aacuguugug     1380

guucugggga gccaagaggg agcuguucau acggcccucg cuggagcuuu ggaggcugag     1440

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuaggu ugaagggugu gucauauucc cuguguaccg cagcguucac auuuaccaag     1560

gucccagcug aaacauugca uggaacaguc acaguggagg ugcaguaugc agggacagac     1620

ggacccugca aagucccagc ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaucc ugugaucacu gaaaguacug agaauucaaa gaugauguug     1740

gagcucgacc caccauuugg ggauucuuac auugucauag gagucgggga caagaaaauc     1800

acccaucacu ggcaucggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggggac acagccuggg acuuuggauc aguugggggu     1920

guguuuaauu cauuggguaa ggguauucac cagaucuuug gagcagcuuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cacuguuggu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuuag cccugggggg agugaugauc     2100

uuccuuucca cggcuguuuc ugcugauguu gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3181
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 1 wild type

<400>  3181
gggagaaagc uuaccaugag aauuaucaau gcuaggaagg agaggaagag acguggcgcu       60

gacaccagca ucggaaucgu cggccuccug cugaccacag ccauggcagc cgagaucacc      120

agacguggga gugcauacua cauguacuug gacaggagcg augcugguaa ggccauuucu      180

uuugcuacca cauugggggu gaacaaaugc cauguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccc augcuggacg agggagugga gccagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaacauaaa      360

aaaggugaag cacgacgauc cagaagagcc gugacgcuuc cuucucacuc cacaaggaag      420

cugcaaacgc gaucacagac uuggcuagaa ucaagagaau acacaaagca ccugaucaag      480

guugagaauu ggauauucag gaaccccggg uuugcgcuag uggcuguagc uauugccugg      540

cuccugggaa gcucgacgag ccaaaaaguc auauacuugg uaaugauauu guugauugcc      600

ccggcauaca gcaucaggug cauaggaguu agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggggguu gcgucaccgu gauggcacag      720

gacaagccaa caguugacau cgaguugguc acgacaacgg uuagcaacau ggccgaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cuguccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa gagaacauug      900

guggauagag guuggggaaa ugggugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cgugcuccaa gaaaaugaca ggcaagagca uccagccgga gaacuuggag     1020

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacaua     1080

ggacaugaaa cugacgaaaa cagagcaaaa gucgagguca cacccaauuc accaagagca     1140

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu     1200

gacuucucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaggag     1260

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg     1320

aacaacaaag aggcauuggu ggaguuuaag gacgcccacg ccaagaggca aacuguugug     1380

guucugggga gccaagaggg agcuguucac acggcccucg cuggagcuuu ggaggcugag     1440

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuaggu ugaagggugu gucauauucc cuguguaccg caguguguac cgcagcgaag     1560

gucccagcug aaacauugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guagacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc ugugaucacu gaaaguacug agaauucaaa gaugauguug     1740

gagcucgacc caccauuugg ggauucuuac auugucauag gagucgggga caagaaaauc     1800

acccaucacu ggcaucggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggggac acagccuggg acuuuggauc aguugggggu     1920

guguuuaauu cauuggguaa ggguauucac cagaucuuug gagcagcuuu uaaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cacuguuggu gugguuaggu     2040

cugaacacaa agaauggauc uaucucccuc acaugcuuag cccugggggg agugaugauc     2100

uuccuuucca cggcuguuuc ugcugauguu gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3182
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 1 wild type

<400>  3182
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggagcu       60

gaugccagca ucggaaucgu cagccuccug cugacuacag ucauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gacaggagcg augcugguaa ggccauuucu      180

uucguuacca cacugggggu gaacaaaugc caugugcaga ucauggaccu cgggcauaug      240

ugugacgcca ccaugaguua ugagugcccc augcuggacg agggagugga gccagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacgacgauc cagaagagcc gugacgcuuc cuucucacuc uacaaggaag      420

uugcaaacgc gaucgcagac uuggcuagaa ucaagagaau acacaaagca ccugaucaag      480

guugagaauu ggauauucag gaaccccggg uuugcgcuag uggcuguagc uauugccugg      540

cuccugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauauu guugauugcc      600

ccggcauaca guaucaggug cauaggaguu agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggagguu gugucaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacaacgg uuagcaacau ggccgaggug      780

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucuguaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cuuuuuggca aggggagcuu ggugacgugu      960

gccaaguuua caugcuccaa gaaaaugaca gggaagagca uccagccgga gaacuuggag     1020

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacgaa     1080

aacagagcaa aagucgaggu uacacccaau ucaccaagag cagaagcaac cuugggaggu     1140

uuuggaagcc ugggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uuaccuuggc auucuggugc agacacugaa acuccacacu ggaacaacaa agaggcacug     1320

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagaa     1380

ggagccguuc acacggcucu cgcuggagcu cuggaggcug agauggaugg ugcgaaggga     1440

aggcuauccu caggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu     1500

gugucauauu cccuguguac cgcagcguuc acauucacca agguuccagc ugaaacauug     1560

cauggaacag ucacagugga ggugcaguau gcagggaggg auggacccug caagguccca     1620

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aacggcuaac     1680

ccugugauca cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1800

agugguagca ucaucggaaa ggcauuugaa gccacuguga gaggcgccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguugggg guguguuuaa cucauugggc     1920

aaggguauuc accagaucuu uggagcagcu uucaaaucac uguucggagg aauguccugg     1980

uucucacaga uccucauagg cacacuguug gugugguugg gucugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu ggccuuggga ggagugauga ucuuccuuuc cacggcuguu     2100

ucugcugaug ugggguguuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3183
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 1 wild type

<400>  3183
gggagaaagc uuaccaugag aauuaucaau gcuaggaagg agaggaagag acguggcgcu       60

gacaccagca ucggaaucgu cggccuccug cugaccacag ccauggcagc cgagaucacu      120

agacguggga gugcauacua cauguacuug gacaggagcg augcugguaa ggccauuucu      180

uuugccacca cauugggggu gaacaaaugc cauguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccc augcuagacg agggagugga gccagaugac      300

gucgauugcu ggugcaacac gacaucgacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacgacgauc cagaagagcc gugacgcuuc cuucucacuc cacgaggaag      420

cugcaaacgc gaucgcagac uuggcuagag ucaagagaau acacaaagca ccugaucaag      480

guugaaaauu ggauauucag gaaccccggg uuugcgcuag uggcuguagc uauagccugg      540

cuccugggaa gcucgacgag ccaaaaaguu auauacuugg ucaugauauu guugauugcc      600

ccggcauaca gcauuaggug cauaggaguu agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggggguu gcgucaccgu gauggcacag      720

gacaagccaa caguugauau cgaguugguc acgacaacgg uuagcaacau ggccgaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg uuguccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa gagaacauug      900

guggauagag guuggggaaa ugggugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cgugcuccaa gaaaaugaca ggcaagagca uccagccgga gaacuuggag     1020

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacgaa     1080

aacagagcaa aagucgaggu cacacccaau ucaccaagag cagaagcaac cuugggaggu     1140

uuuggaagcu ugggacuuga cugugaacca aggacaggcc uugacuucuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaagg agugguuuca ugacauccca     1260

uuaccuuggc augcuggugc agacacugga acuccacacu ggaacaacaa agaggcauug     1320

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagag     1380

ggagcuguuc auacggcccu cgcuggagcu uuggaggcug agauggaugg ugcaaaggga     1440

aggcuauucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu     1500

gugucauauu cccuguguac cgcagcguuc acauuuacca aggucccagc ugaaacauug     1560

cauggaacag ucacagugga ggugcaguau gcagggacag acggacccug caaaguccca     1620

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaau     1680

ccugugauca cugaaaguac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1800

agugguagca ccaucggaaa ggcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuuggggg acacagccug ggacuuugga ucaguugggg guguguuuaa uucauugggu     1920

aaggguauuc accagaucuu uggagcagcu uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacacuguug gugugguuag guuugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu agcccugggg ggagugauga ucuuccuuuc cacggcuguu     2100

ucugcugaug uugggugcuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3184
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 1 wild type

<400>  3184
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugaga cacggcgauc uagaagaucu gugucgcucc guuaucacua uacaaggaag      420

uugcaaacgc ggucgcagac augguuagaa ucaagagaau acaagaagca cuugaucaug      480

gucgaaaacu ggauauucag gaaccccggg uuugccauag uguccguugc cauuaccugg      540

cugaugggaa gcuugacgag ccaaaaaguc auauacuugg ucaugauagu guugauuguc      600

ccggcauaca guaucagcug cauuggaguc agcaauagag acuuagugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggagggu gcguuaccga gauggcacag      720

gacaagccaa caguugacau agaguugguc acgaugacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucguuaucc gacauggcuu cggccagucg uugcccaaca      840

caaggcgaac ccucccucga caagcaauca gacacucaau cuguaugcaa aagaacauua      900

ggagacagag guuggggaaa ugguuguggg auuuuuggca aagggagcuu ggugacaugu      960

uccaaguuca cguguuguaa gaagaugccc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcucccagu gcauggcucc cagcauagcg ggaugauugu gaaugacaua     1080

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguca cacccaauuc accaagagca     1140

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu     1200

gacuucucag aucuguauua ucugaccaug aacaacaagc auugguuggu gcacaaggag     1260

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg     1320

aacaacaaag aggcauuggu ggaguucaag gacgcccacg ccaagaggca aacuguugug     1380

guucugggga gccaagaggg agcuguucac acggcccucg cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuaggu ugaagggugu gucauauucc cuguguacug cagcguucac auuuaccaag     1560

gucccagcug aaacauugca uggaacaguu acaguggagg ugcagucugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3185
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 1 wild type

<400>  3185
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca caaugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcccuc aagaagagcu gucacgcuuc cuucccauuc cacaagaaag      420

uuacagacac ggucacaaac cuggcuugaa ucacgggagu acacgaagca ccugaucaaa      480

guugagaacu ggauacuccg gaauccaggg uucacaguug cugcucuagc aauggccugg      540

cucuugggaa auuccacgag ccagcgagug aucuaucugg ugaugauccu guugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga gaauuuggag     1020

uacagaauua ugcugucagu gcacggcucc caacacagcg ggaugauugu gaaugacaua     1080

ggacaugaga ccgaugaaaa cagagcaaaa guggagguca caccaaacuc accaagagcu     1140

gaagcgaccc uagggggcuu cgggaguuug ggacucgauu gugagccaag gaccgguuua     1200

gauuucuccg accuguacua uuuaaccaug aacaacaagc auugguuggu gcacaaggag     1260

ugguuccacg acauuccauu gccauggcac gcuggagcgg acacagggac uccccauugg     1320

aacaacaaag aggcauuggu ugaguucaag gaugcccacg ccaaaagaca gacuguuguc     1380

guguugggaa gucaggaggg ugccguccac acagcccugg caggagcuuu ggaagcggag     1440

auggauggag ccaaaggaag gcuguucuca ggucauuuga agugccggau aaagauggac     1500

aagcuuaggu ugaagggugu cucauacuca uugugcacug ccgccuucac auucucaaag     1560

guuccggcug aaacgcugca ugggacaguc acaguugagg ugcaguaugc gggcagagau     1620

ggaccuugca aggugccagc ccaaauggca guggauaugc agacucuuac cccaguggga     1680

agguugauaa cggcaaaucc caucauaacu gagagcacug aaaacucgag aaugauguug     1740

gagcuggacc cacccuuugg agacucuuac auugucauug ggguuggaga uaagaaaauu     1800

acccaucauu ggcacagaag uggaagcacg auugggaagg cauuugaggc cacugugaga     1860

ggugcaaaaa ggauggcagu ccugggagau accgccuggg auuuugguuc uguuggaggc     1920

guguucaauu cucugggcaa gggaguucau caggucuucg gagcagccuu uaaauccuua     1980

uuuggcggaa uguccugguu uucacaaauu cuuauuggca cacuuuuagu gugguugggc     2040

uugaauacaa aaaauggauc cauuucccuu gcaugucugg cgcuuggagg ggcgaugauc     2100

uuucucucca cggccgucuc ggcagacgug gggugcucag uugacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  3186
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 1 wild type

<400>  3186
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3187
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 1 wild type

<400>  3187
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3188
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 1 wild type

<400>  3188
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagc uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuc aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuua gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3189
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 1 wild type

<400>  3189
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3190
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 1 wild type

<400>  3190
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auugucaaug auacaggaua ugaaacugac gaaaauagag cgaaagucga gguuacgccu     1080

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa gcuuaggacu ugacugugaa     1140

ccaaggacag gccuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaggca uugguagaau ucaaggaugc ccacgccaag     1320

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaagcugu ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug cacugcggca     1500

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagauc ccaguccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaggccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3191
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 1 wild type

<400>  3191
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc gcaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguu gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auugucaaug auacaggaua ugaaacugac gaaaauagag cgaaagucga gguuacgccu     1080

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa gcuuaggacu ugacugugaa     1140

ccaaggacag gucuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaggca uugguagaau ucaaggaugc ccacgccaag     1320

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaagcugu ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug cacugcggca     1500

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagauc ccaguccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaggccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3192
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 1 wild type

<400>  3192
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguu gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

acugucaaug auauaggaua ugaaacugac gaaaauagag cgaaagucga gguuacgccu     1080

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa gcuuaggacu ugacugugaa     1140

ccaaggacag gccuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacu     1260

ggaacuccac acuggaacaa caaagaggca uugguagaau ucaaggaugc ccacgccaag     1320

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaagcugu ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug cacugcggca     1500

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagauc ccaguccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaggccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3193
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 1 wild type

<400>  3193
gggagaaagc uuaccaugac cagcaucgga aucguuggcc uccuguugac uacagccaug       60

gcagcagaga ucacuagacg ugggagugca uacuacaugu acuuggauag gagcgaugca      120

gggaaggcca uuucuuucgc uaccacauug ggggugaaca aaugccaugu gcagaucaug      180

gaccucgggc acauguguga cgccaccaug agcuaugaau gcccuaugcu ggacgagggg      240

guggaaccag augacgucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc auaaaaaagg ugaagcacgg cgaucuagaa gagccgucac gcucccaucu      360

cacuccacaa ggaaauugca aacgcggucg cagacuuggc uagaaucaag agaauacaca      420

aagcaccuga ucaagguuga aaauuggaua uucaggaacc cugguuuuac gcuaguggcu      480

gucgccaucg ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauag gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gacguugucc uggaacaugg aggcugcguc      660

accgugaugg cacaggacaa gccaacaguu gacauagagc uggucacaac aacgguuagu      720

aacauggccg aggugagauc cuauuguuac gaggcaucaa uaucggacau ggcuucggac      780

agucgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguu      840

ugcaaaagaa cauuggugga cagagguugg ggaaaugggu guggacucuu uggcaaaggg      900

aguuugguga caugugcuaa guucacgugc uccaagaaga ugacugggaa gagcauucag      960

ccggagaacc uggaguaucg gauaaugcua ucagugcaug gcucccagca cagugggaug     1020

auuguuaaug augaaaacag agcgaagguc gagguuacgc ccaauucacc aagagcagaa     1080

gcaacccugg gaggcuuugg aagcuuagga cuugauugug aaccaaggac aggccuugac     1140

uuuucagauc uguauuaccu aaccaugaau aacaagcauu gguuggugca caaagagugg     1200

uuucaugaca ucccauugcc cuggcaugcu ggggcagaca cuggaacucc acauuggaac     1260

aacaaggagg cauuagugga auucaaggac gcccacgcca agaggcaaac cgucgugguu     1320

uuggggagcc aggaaggagc cguccacacg gcucuugcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaggcu auucucuggc cacuugaaau gucgcuuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcaccgcgg cauucacauu caccaagguc     1500

ccggcugaaa cacuacaugg aacagucaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaagg ucccagccca gauggcggug gacaugcaga ccuugacccc agucggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag uuggggauaa gaaaaucacc     1740

caucacuggc auaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggc     1800

gcuaagagaa uggcaguccu gggggacaca gcuugggacu uuggaucagu cggaggugug     1860

uuuaacucau ugggcaaggg cauucaucag auuuuuggag cagcuuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacuc ugcuggugug guuaggucug     1980

aacacaaaga augggucuau cucccucaca ugcuuagccc uggggggagu gaugaucuuc     2040

cucuccacgg cuguuucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3194
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 1 wild type

<400>  3194
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaccg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga aaauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagc uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuc aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaagcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuua gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3195
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 1 wild type

<400>  3195
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaggggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3196
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 1 wild type

<400>  3196
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua gaaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggccucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3197
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 1 wild type

<400>  3197
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccuacugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacugguac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3198
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 1 wild type

<400>  3198
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccacuaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa uaaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga cacgcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3199
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 1 wild type

<400>  3199
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agauaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguggagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagcu     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3200
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 1 wild type

<400>  3200
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu cuagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3201
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 1 wild type

<400>  3201
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuacgcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccagc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc uggucacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca guaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3202
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 1 wild type

<400>  3202
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cugauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu ccuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacaguc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3203
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 1 wild type

<400>  3203
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu cagcuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggccg caaaccuggu uggaaucaag agaauacaca      420

aagcauuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acaagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3204
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 1 wild type

<400>  3204
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaaguuca acgagcaaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau ugggacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3205
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 1 wild type

<400>  3205
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgcgccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cugauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu ccuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3206
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 1 wild type

<400>  3206
gggagaaagc uuaccaugac uagugucgga aucguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg ggguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gcuuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggacucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3207
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 1 wild type

<400>  3207
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacca ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcauuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acaagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagccuc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucugac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3208
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 1 wild type

<400>  3208
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggauag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagccgugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagaaucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugauaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcccaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3209
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 1 wild type

<400>  3209
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugagu gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua cucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua uuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu cagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaauc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3210
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 1 wild type

<400>  3210
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuucaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaacaa uaucagacau agcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ccgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3211
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 1 wild type

<400>  3211
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacca ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucugac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccga gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggaguuacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3212
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 1 wild type

<400>  3212
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc gcuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auauagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc caccuugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cauugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau cggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3213
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 1 wild type

<400>  3213
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga ugguguaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaaaauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuuag gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu ccuuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3214
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 1 wild type

<400>  3214
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg ccuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaagcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3215
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 1 wild type

<400>  3215
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaaagaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagccgugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cugguuucgc uuuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguu      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaauguug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcau aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3216
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 1 wild type

<400>  3216
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gcuuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggaagc acaugcuaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3217
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 1 wild type

<400>  3217
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acaugugcga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aauacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cgcccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaacucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3218
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 1 wild type

<400>  3218
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucaccagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuggugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcauugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcagggg cagauggacc cugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3219
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 1 wild type

<400>  3219
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagccgugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cugguuucgc uuuagcagca      480

gcugccaucg cguggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugcccuggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaauguug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcccaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3220
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 1 wild type

<400>  3220
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcagagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau gguuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu cucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugc ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3221
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 1 wild type

<400>  3221
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccauag cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gaaucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaguauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa uccuaggacu ggauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3222
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 1 wild type

<400>  3222
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggaag ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccauua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcauggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3223
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 1 wild type

<400>  3223
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuccc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacag agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aaguuauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg aggcuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ccaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ggauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagauacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3224
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 1 wild type

<400>  3224
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcc      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca agcgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auauagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguu gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agcuugguga caugcgcuaa guuugcaugc uccaagaaga ugaccgggaa gagcauccag      960

ccagagaauu uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaacagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aagagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggugcucu uaacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3225
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 1 wild type

<400>  3225
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcagcggaag ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuggcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauucu ccaagauccc ggcugaaaca cugcacggga cagucacaga ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccgguaa ucacugaaag cacagagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa uacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3226
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 1 wild type

<400>  3226
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agauccagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucaguaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaacaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacacugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaagg cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcguucu uaacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa uacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucuccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3227
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 1 wild type

<400>  3227
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggagucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagauac ccaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggaaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguccaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggacaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc cugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca guaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggccugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3228
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 1 wild type

<400>  3228
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcaguggagg ucacuagacg ugggaaugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacaaug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcaccuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcuugc ucuaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug auacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcaugcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcagacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca cuaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

uccaagauga ugcuggaacu ggauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggaaaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug ggggugcucu caacucacug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugguguggu ugggucugaa uacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3229
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 1 wild type

<400>  3229
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcgcgg agaucuagaa gagcugugac gcuccccucc      360

cacuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuggcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auauagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gaugucgucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcag agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgccaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg aauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccuggga gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggaugc acaugccaaa     1320

aggcaaacug ucgugguccu agggagucaa gagggagcag uucacacggc ucuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagacugaag ggcgugucau acucccugug uaccgcagcg     1500

uucacauuca cuaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggua uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa uacaaagaau ggaucuauuu cccuugugug cuuggccuua     2040

gggggagugu ugaucuucuu guccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3230
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 1 wild type

<400>  3230
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcgcgg agaucuagaa gagcugugac gcuccccucc      360

cacuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuggcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auauagcauc aggugcauag gagucaacaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gaugucgucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcag agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgccaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg aauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccuggga gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggaugc acaugccaaa     1320

aggcaaacug ucgugguccu agggagucaa gagggagcag uucacacggc ucuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagacugaag ggcgugucau acucccugug uaccgcagcg     1500

uucacauuca cuaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggua uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa uacaaagaau ggaucuauuu cccuugugug cuuggccuua     2040

gggggagugu ugaucuucuu guccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3231
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 1 wild type

<400>  3231
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcaguggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaca aguguuacau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuauguu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacau caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauauaca      420

aagcaccuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg ccuggcuuuu gggaaguuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa accggcuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuauugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagcugaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcaugcugg ggcagacacc     1260

ggaacuccac auuggaacaa caaagaagca uugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc cugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggaggcu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ucacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggauuuu     1860

ggaucaguug gaggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugguguggu ugggucugaa uacaaagaau ggaucuauuu cccuuacgug cuuggccuua     2040

gggggagugu ugaucuuuuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3232
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 1 wild type

<400>  3232
gggagaaagc uuaccaugac uagcgucgga auuguuggcc uccuccugac cacagccaug       60

gcaguagagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuacau acaaaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuauguu ggaugagggg      240

guagaaccag augacgucga uugcuggugc aacacgacau caacuugggu uguguaugga      300

accugccacc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcaccuga uuagaguuga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugucaucg cuuggcuuuu gggaaguuca acgagccaaa aagucauaua ucuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggauau ggcuucggac      780

agccgcugcc caacacaagg ugaggccuac cuugacaagc agucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggga gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuaccuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcaugcugg ggcagacacu     1260

ggaacuccac auuggaacaa caaagaagca cugguagagu ucaaggacgc acaugcaaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggagccaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug cacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga uaugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag caccgagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ucacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug ggggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguucgg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugguguggu ugggucugaa uacaaagaau ggaucuauuu cccuuacgug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3233
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 1 wild type

<400>  3233
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagccaug       60

gcaguggagg ucaccagacg ugggagugca uacuauaugu acuuagacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug ggggugaaua aguguuacau acagaucaug      180

gaucuuggac acauguguga ugccacaaug agcuaugaau gcccuauguu ggaugagggg      240

guagaaccag augacgucga uugcuggugc aacacgacau cgacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaggcacgg agaucuagaa gagcugugac gcuccccucu      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga ucagagucga aaauuggaua uucaggaacc cuggcuuugc guuggcagca      480

gcugccauug cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacuguuga uugccccggc auacaguauc aggugcauag gagucagcaa uagggauuuu      600

guggaaggua ugucaggugg gaccuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa gccaacuguu gauauagagu uggucacaac aacgguuagc      720

aacauggcgg agguaagauc cuacugcuac gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguu      840

ugcaaaagaa cguuagugga cagagguugg ggaaauggau guggacucuu uggcaaaggg      900

agccugguga caugcgccaa guuugcaugc uccaagaaaa ugacugggaa gagcauccag      960

ccagagaacc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

auuguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccuggga gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcauugg     1200

uuggugcaca aggagugguu ccaugacauu ccacuaccuu ggcaugcugg ggcagacacc     1260

ggaacuccac auuggaacaa caaagaagca uugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagccg uucacacggc ucuugcugga     1380

gcccuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgcuugaaaa uggacaaacu uagauugaag ggcgugucau acuccuuaug uaccgcggcg     1500

uucacauuca ccaagauccc ggcugaaacg cugcauggga cagucacagu ggagguacag     1560

uaugcaggga cagauggacc cugcaagguu ccagcucaga uggcggugga uaugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguga ucacugaaag cacugagaau     1680

ucaaagauga uguuggaacu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggauaaga agaucaccca ccacugguac aggaguggca gcaccaucgg aaaagcauuu     1800

gaagccacug ugagaggcgc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucagucg gaggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau aggaacguug     1980

cugguguggu ugggucugaa cacaaagaau ggaucuauuu cccuuacgug cuuggccuua     2040

gggggagugu ugaucuuccu aucuacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3234
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 1 wild type

<400>  3234
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagccaug       60

gcaguggagg ucaccagacg ugggagugca uacuauaugu acuuagacag aagcgaugcu      120

gggaaggcca uaucuuuucc aaccacacug ggggugaaua aguguuacau acagaucaug      180

gaucuuggac acauguguga ugccacaaug agcuaugaau gcccuauguu ggaugagggg      240

guagaaccag augacgucga uugcuggugc aacacgacau cgacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaggcacgg agaucuagaa gagcugugac gcuccccucu      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga ucagagucga aaauuggaua uucaggaacc cuggcuuugc guuggcagca      480

gcugccauug cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacuguuga uugccccggc auacaguauc aggugcauag gagucagcaa uagggauuuu      600

guggaaggua ugucaggugg gaccuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa gccaacuguu gauauagagu uggucacaac aacgguuagc      720

aacauggcgg agguaagauc cuacugcuac gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguu      840

ugcaaaagaa cguuagugga cagagguugg ggaaauggau guggacucuu uggcaaaggg      900

agccugguga caugcgccaa guuugcaugc uccaagaaaa ugacugggaa gagcauccag      960

ccagagaacc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

auuguuaaug acauaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccuggga gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcauugg     1200

uuggugcaca aggagugguu ccaugacauu ccacuaccuu ggcaugcugg ggcagacacc     1260

ggaacuccac auuggaacaa caaagaagca uugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagccg uucacacggc ucuugcugga     1380

gcccuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgcuugaaaa uggacaaacu uagauugaag ggcgugucau acuccuuaug uaccgcggcg     1500

uucacauuca ccaagauccc ggcugaaacg cugcauggga cagucacagu ggagguacag     1560

uaugcaggga cagauggacc cugcaagguu ccagcucaga uggcggugga uaugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguga ucacugaaag cacugagaau     1680

ucaaagauga uguuggaacu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggauaaga agaucaccca ccacuggcac aggaguggca gcaccaucgg aaaagcauuu     1800

gaagccacug ugagaggcgc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucagucg gaggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau aggaacguug     1980

cugguguggu ugggucugaa cacaaagaau ggaucuauuu cccuuacgug cuuggccuua     2040

gggggagugu ugaucuuccu aucuacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3235
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 1 wild type

<400>  3235
gggagaaagc uuaccaugac cagcaucgga aucguuggcc uccuguugac uacagccaug       60

gcagcagaga ucacuagacg ugggagugca uacuacaugu acuuggauag gagcgaugca      120

gggaaggcca uuucuuucgc uaccacauug ggggugaaca aaugccaugu gcagaucaug      180

gaccucgggc acauguguga cgccaccaug agcuaugaau gcccuaugcu ggacgagggg      240

guggaaccag augacgucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc auaaaaaagg ugaagcacgg cgaucuagaa gagccgucac gcucccaucu      360

cacuccacaa ggaaauugca aacgcggucg cagacuuggc uagaaucaag agaauacaca      420

aagcaccuga ucaagguuga aaauuggaua uucaggaacc cugguuuuac gcuaguggcu      480

gucgccaucg ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauag gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gacguugucc uggaacaugg aggcugcguc      660

accgugaugg cacaggacaa gccaacaguu gacauagagc uggucacaac aacgguuagu      720

aacauggccg aggugagauc cuauuguuac gaggcaucaa uaucggacau ggcuucggac      780

agucgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguu      840

ugcaaaagaa cauuggugga cagagguugg ggaaaugggu guggacucuu uggcaaaggg      900

aguuugguga caugugcuaa guucacgugc uccaagaaga ugacugggaa gagcauucag      960

ccggagaacc uggaguaucg gauaaugcua ucagugcaug gcucccagca cagugggaug     1020

auuguuaaug acacaggaca ugaaacugau gaaaacagag cgaaggucga gguuacgccc     1080

aauucaccaa gagcagaagc aacccuggga ggcuuuggaa gcuuaggacu ugauugugaa     1140

ccaaggacag gccuugacuu uucagaucug uauuaccuaa ccaugaauaa caagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugcccu ggcaugcugg ggcagacacu     1260

ggaacuccac auuggaacaa caaggaggca uuaguggaau ucaaggacgc ccacgccaag     1320

aggcaaaccg ucgugguuuu ggggagccag gaaggagccg uccacacggc ucuugcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaggcuau ucucuggcca cuugaaaugu     1440

cgcuuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug caccgcggca     1500

uucacauuca ccaagguccc ggcugaaaca cuacauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagguc ccagcccaga uggcggugga caugcagacc     1620

uugaccccag ucggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaau     1680

ucaaagauga uguuggagcu cgacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggauaaga aaaucaccca ucacuggcau aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggcgc uaagagaaug gcaguccugg gggacacagc uugggacuuu     1860

ggaucagucg gagguguguu uaacucauug ggcaagggca uucaucagau uuuuggagca     1920

gcuuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacucug     1980

cugguguggu uaggucugaa cacaaagaau gggucuaucu cccucacaug cuuagcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3236
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 1 wild type

<400>  3236
gggagaaagc uuaccaugac cagcaucgga aucauugguc uccugcugac uacagccaug       60

gcagcggaga uuaccagacg uggaagugca uacuacaugu acuuggacag gagcgaugcu      120

gggaaggcca ucuccuuugc uaccaacuug ggaguuaaca aaugccaugu acagaucaug      180

gaucucgggc acauguguga cgccaccaug aguuaugagu gcccuauguu ggacgaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaagcacgg cgaucuagaa gagcugugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcaccuga ucaagguuga aaacuggaua uuuaggaacc ccggguuugc gcucgcagcu      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuugguuaug      540

auacugcuga uugccccggc auacagcauc aggugcauug gagucagcaa uagagacuuc      600

guggaaggca ugucaggugg gaccuggguu gaugucgucu uggaacaugg agguugcguc      660

accguuaugg cacaggacaa gccaacaguu gacauagagu uggucacgac aacgguuagu      720

aacauggccg agguaagauc cuacuguuau gaggcaucaa uaucagacau ggcuucggac      780

agucgcugcc caacacaagg ugaagccuac cuugauaagc aaucagacac ccaauaugug      840

ugcaaaagaa cauuggugga cagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agccugguga caugugccaa guucacgugu uccaagaaga ugacugggaa gagcauucag      960

ccggagaauc uggaguaucg aauaaugcua ucagugcaug gcucccagca cagcgggaug     1020

auuguuaaug acauaggaca ugaaacugac gaaaacagag cgaaagucga ggucacgccc     1080

aauucaccaa gagcagaagc aaccuuggga ggcuuuggaa gcuuaggacu ugauugugaa     1140

ccaaggacag gccuggacuu uucagauuug uacuacuuga ccaugaacaa caagcacugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagauacc     1260

ggaacuccac acuggaacaa caaggaggca uugguagagu ucaaagaugc ccacgccaag     1320

aggcaaaccg ucguuguucu agggagccag gaaggagccg uucacacggc ucuugcugga     1380

gcucuggagg cugagaugga uggugcaaaa ggaaggcugu ucuccggcca uuugaaaugc     1440

cgcuuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug caccgcagcg     1500

uucacauuua ccaagguccc agcugaaaca uugcauggaa caguuacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagguc ccagcccaga uggcggugga caugcagacc     1620

cugaccccag ucgggaggcu gauaaccgcc aacccuguga uuacugaaag cacugagaau     1680

ucaaagauga uguuggagcu cgacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ucacuggcau aggaguggua gcaccaucgg aaaagcauuu     1800

gaggccacug ugagaggugc caagagaaug gcaguucugg gggauacagc cugggacuuc     1860

ggaucagucg ggggcguguu caacucauug ggcaagggug uucaccagau uuuuggagca     1920

gcuuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cugguguggu uggguuugaa cacaaagaau ggauccaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3237
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 1 wild type

<400>  3237
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguu gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaaugguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guucacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auugucaaug auauaggaca ugaaacugac gaaaacagag cgaaagucga gguuacgccu     1080

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa gcuuaggacu ugacugugaa     1140

ccaaggacag gccuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaggca uugguagaau ucaaggaugc ccacgccaag     1320

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaggcugu ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug cacugcggca     1500

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagguc ccagcccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaagccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3238
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 1 wild type

<400>  3238
gggagaaagc uuaccaugac cagcaucgga aucauugguc uccugcugac uacagccaug       60

gcagcagaga uuaccagacg uggaagugca uacuacaugu acuuggacag gagcgaugcu      120

gggaaggcca ucuccuuugc uaccaauuug ggaguuaaca aaugccaugu acagaucaug      180

gaucucgggc acauguguga cgccaccaug aguuaugagu guccuauguu ggacgaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaagcacgg cgaucuagaa gagcugugac gcucccuucu      360

cacucuacaa ggaagcugca aacgcggucg cagacuuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcucgcagcu      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauug gagucagcaa uagagacuuc      600

guggaaggca ugucaggugg gaccuggguu gaugucgucu uggaacaugg agguugcguc      660

accguaaugg cacaggacaa gccaacaguu gacauagagu uggucacgac aacggucagu      720

aacauggccg agguaagauc cuacuguuau gaggcaucaa uaucggacau ggcuucggac      780

agucgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauaugua      840

ugcaaaagaa cauuggugga cagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agccugguga caugugccaa guucacgugu uccaagaaga ugaccgggaa gagcauucag      960

ccggagaauc uggaguaucg aauaaugcua ucagugcaug gcucccagca cagcgggaug     1020

auuguuaaug acgaaaauag ggcgaaaguc gaggucacgc ccaauucacc aagagcagaa     1080

gcaaccuugg gaggcuuugg aagcuuagga cuugauugug aaccaaggac aggccuggac     1140

uuuucagauu uguacuacuu gaccaugaac aauaagcacu gguuagugca caaagagugg     1200

uuucaugaca ucccauugcc cuggcaugcu ggggcagaca ccggaacucc acauuggaac     1260

aacaaggagg cauugguaga guucaaagau gcccacgcua agaggcaaac cgucguuguu     1320

cuagggagcc aggaaggagc cguucacacg gcucuugcug gagcucugga ggcugagaug     1380

gauggugcaa aaggaagguu guucuccggc cauuugaaau gccgcuuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcaccgcag cguucacauu caccaagguc     1500

ccagcugaaa cauugcaugg aacaguuaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaagg ucccagcuca gauggcggug gacaugcaga cccugacccc agucggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag uuggggacaa gaaaauuacc     1740

caccacuggc auaggagugg uagcaccauc ggaaaagcau uugaggccac ugugagaggu     1800

gccaagagaa uggcaguucu gggggacaca gccugggacu uuggaucagu cgggggcgug     1860

uuuaacucau ugggcaaggg cauccaccag auuuuuggag cagccuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacgc ugcuggugug guuggguuug     1980

aacacaaaga auggauccau cucccucaca ugcuuggccc uggggggagu gaugaucuuc     2040

cucuccacgg cugucucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3239
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 1 wild type

<400>  3239
gggagaaagc uuaccaugac cagcaucgga aucgucggcc uccugcugac cacagccaug       60

gcagccgaga ucacuagacg ugggagugca uacuacaugu acuuggacag gagcgaugcu      120

gguaaggcca uuucuuuugc caccacauug ggggugaaca aaugccaugu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gccccaugcu agacgaggga      240

guggagccag augacgucga uugcuggugc aacacgacau cgacuugggu uguguacgga      300

accugucauc auaaaaaagg ugaagcacga cgauccagaa gagccgugac gcuuccuucu      360

cacuccacga ggaagcugca aacgcgaucg cagacuuggc uagagucaag agaauacaca      420

aagcaccuga ucaagguuga aaauuggaua uucaggaacc ccggguuugc gcuaguggcu      480

guagcuauag ccuggcuccu gggaagcucg acgagccaaa aaguuauaua cuuggucaug      540

auauuguuga uugccccggc auacagcauu aggugcauag gaguuagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg ggguugcguc      660

accgugaugg cacaggacaa gccaacaguu gauaucgagu uggucacgac aacgguuagc      720

aacauggccg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccguuguc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguc      840

ugcaagagaa cauuggugga uagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugc uccaagaaaa ugacaggcaa gagcauccag      960

ccggagaacu uggaguaccg gauaaugcua ucagugcaug gaucccagca cagugggaug     1020

auugugaaug acacaggaca ugaaacugac gaaaacagag caaaagucga ggucacaccc     1080

aauucaccaa gagcagaagc aaccuuggga gguuuuggaa gcuugggacu ugacugugaa     1140

ccaaggacag gccuugacuu cucagaucug uauuacuuga ccaugaacaa uaagcauugg     1200

uuggugcaca aggagugguu ucaugacauc ccauuaccuu ggcaugcugg ugcagacacu     1260

ggaacuccac acuggaacaa caaagaggca uugguggagu ucaaggacgc ccacgccaag     1320

aggcaaacug uugugguucu ggggagccaa gagggagcug uucauacggc ccucgcugga     1380

gcuuuggagg cugagaugga uggugcaaag ggaaggcuau ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagguugaag ggugugucau auucccugug uaccgcagcg     1500

uucacauuua ccaagguccc agcugaaaca uugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagacggacc cugcaaaguc ccagcccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aauccuguga ucacugaaag uacugagaau     1680

ucaaagauga uguuggagcu cgacccacca uuuggggauu cuuacauugu cauaggaguc     1740

ggggacaaga aaaucaccca ucacuggcau cggaguggua gcaccaucgg aaaggcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gggacacagc cugggacuuu     1860

ggaucaguug gggguguguu uaauucauug gguaagggua uucaccagau cuuuggagca     1920

gcuuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacacug     1980

uugguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuagcccug     2040

gggggaguga ugaucuuccu uuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3240
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 1 wild type

<400>  3240
gggagaaagc uuaccaugac cagcaucgga aucgucggcc uccugcugac cacagccaug       60

gcagccgaga ucaccagacg ugggagugca uacuacaugu acuuggacag gagcgaugcu      120

gguaaggcca uuucuuuugc uaccacauug ggggugaaca aaugccaugu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gccccaugcu ggacgaggga      240

guggagccag augacgucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucaac auaaaaaagg ugaagcacga cgauccagaa gagccgugac gcuuccuucu      360

cacuccacaa ggaagcugca aacgcgauca cagacuuggc uagaaucaag agaauacaca      420

aagcaccuga ucaagguuga gaauuggaua uucaggaacc ccggguuugc gcuaguggcu      480

guagcuauug ccuggcuccu gggaagcucg acgagccaaa aagucauaua cuugguaaug      540

auauuguuga uugccccggc auacagcauc aggugcauag gaguuagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg ggguugcguc      660

accgugaugg cacaggacaa gccaacaguu gacaucgagu uggucacgac aacgguuagc      720

aacauggccg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcuguc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguc      840

ugcaagagaa cauuggugga uagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugc uccaagaaaa ugacaggcaa gagcauccag      960

ccggagaacu uggaguaccg gauaaugcua ucagugcaug gaucccagca cagugggaug     1020

auugugaaug acauaggaca ugaaacugac gaaaacagag caaaagucga ggucacaccc     1080

aauucaccaa gagcagaagc aaccuuggga gguuuuggaa gcuugggacu ugacugugaa     1140

ccaaggacag gccuugacuu cucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aggagugguu ucaugacauc ccauuaccuu ggcaugcugg ugcagacacu     1260

ggaacuccac acuggaacaa caaagaggca uugguggagu uuaaggacgc ccacgccaag     1320

aggcaaacug uugugguucu ggggagccaa gagggagcug uucacacggc ccucgcugga     1380

gcuuuggagg cugagaugga uggugcaaag ggaaggcuau ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagguugaag ggugugucau auucccugug uaccgcagug     1500

uguaccgcag cgaagguccc agcugaaaca uugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagguc ccagcccaga uggcgguaga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aacccuguga ucacugaaag uacugagaau     1680

ucaaagauga uguuggagcu cgacccacca uuuggggauu cuuacauugu cauaggaguc     1740

ggggacaaga aaaucaccca ucacuggcau cggaguggua gcaccaucgg aaaggcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gggacacagc cugggacuuu     1860

ggaucaguug gggguguguu uaauucauug gguaagggua uucaccagau cuuuggagca     1920

gcuuuuaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacacug     1980

uugguguggu uaggucugaa cacaaagaau ggaucuaucu cccucacaug cuuagcccug     2040

gggggaguga ugaucuuccu uuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3241
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 1 wild type

<400>  3241
gggagaaagc uuaccaugac cagcaucgga aucgucggcc uccugcugac cacagccaug       60

gcagccgaga ucacuagacg ugggagugca uacuacaugu acuuggacag gagcgaugcu      120

gguaaggcca uuucuuuugc caccacauug ggggugaaca aaugccaugu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gccccaugcu agacgaggga      240

guggagccag augacgucga uugcuggugc aacacgacau cgacuugggu uguguacgga      300

accugucauc auaaaaaagg ugaagcacga cgauccagaa gagccgugac gcuuccuucu      360

cacuccacga ggaagcugca aacgcgaucg cagacuuggc uagagucaag agaauacaca      420

aagcaccuga ucaagguuga aaauuggaua uucaggaacc ccggguuugc gcuaguggcu      480

guagcuauag ccuggcuccu gggaagcucg acgagccaaa aaguuauaua cuuggucaug      540

auauuguuga uugccccggc auacagcauu aggugcauag gaguuagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg ggguugcguc      660

accgugaugg cacaggacaa gccaacaguu gauaucgagu uggucacgac aacgguuagc      720

aacauggccg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccguuguc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguc      840

ugcaagagaa cauuggugga uagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugc uccaagaaaa ugacaggcaa gagcauccag      960

ccggagaacu uggaguaccg gauaaugcua ucagugcaug gaucccagca cagugggaug     1020

auugugaaug acgaaaacag agcaaaaguc gaggucacac ccaauucacc aagagcagaa     1080

gcaaccuugg gagguuuugg aagcuuggga cuugacugug aaccaaggac aggccuugac     1140

uucucagauc uguauuaccu gaccaugaac aauaagcauu gguuggugca caaggagugg     1200

uuucaugaca ucccauuacc uuggcaugcu ggugcagaca cuggaacucc acacuggaac     1260

aacaaagagg cauuggugga guucaaggac gcccacgcca agaggcaaac uguugugguu     1320

cuggggagcc aagagggagc uguucauacg gcccucgcug gagcuuugga ggcugagaug     1380

gauggugcaa agggaaggcu auucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagguuga aggguguguc auauucccug uguaccgcag cguucacauu uaccaagguc     1500

ccagcugaaa cauugcaugg aacagucaca guggaggugc aguaugcagg gacagacgga     1560

cccugcaaag ucccagccca gauggcggug gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaauccugu gaucacugaa aguacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag ucggggacaa gaaaaucacc     1740

caucacuggc aucggagugg uagcaccauc ggaaaggcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggggacaca gccugggacu uuggaucagu ugggggugug     1860

uuuaauucau uggguaaggg uauucaccag aucuuuggag cagcuuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacac uguuggugug guuagguuug     1980

aacacaaaga auggaucuau cucccucaca ugcuuagccc uggggggagu gaugaucuuc     2040

cuuuccacgg cuguuucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3242
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 1 wild type

<400>  3242
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugagacacgg cgaucuagaa gaucuguguc gcuccguuau      360

cacuauacaa ggaaguugca aacgcggucg cagacauggu uagaaucaag agaauacaag      420

aagcacuuga ucauggucga aaacuggaua uucaggaacc ccggguuugc cauagugucc      480

guugccauua ccuggcugau gggaagcuug acgagccaaa aagucauaua cuuggucaug      540

auaguguuga uugucccggc auacaguauc agcugcauug gagucagcaa uagagacuua      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg agggugcguu      660

accgagaugg cacaggacaa gccaacaguu gacauagagu uggucacgau gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucgu uauccgacau ggcuucggcc      780

agucguugcc caacacaagg cgaacccucc cucgacaagc aaucagacac ucaaucugua      840

ugcaaaagaa cauuaggaga cagagguugg ggaaaugguu gugggauuuu uggcaaaggg      900

agcuugguga cauguuccaa guucacgugu uguaagaaga ugcccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcuc ccagugcaug gcucccagca uagcgggaug     1020

auugugaaug acauaggaca ugaaacugac gaaaacagag cgaaagucga ggucacaccc     1080

aauucaccaa gagcagaagc aaccuuggga gguuuuggaa gcuugggacu ugacugugaa     1140

ccaaggacag gccuugacuu cucagaucug uauuaucuga ccaugaacaa caagcauugg     1200

uuggugcaca aggagugguu ucaugacauc ccauuaccuu ggcaugcugg ugcagacacu     1260

ggaacuccac acuggaacaa caaagaggca uugguggagu ucaaggacgc ccacgccaag     1320

aggcaaacug uugugguucu ggggagccaa gagggagcug uucacacggc ccucgcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcuau ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagguugaag ggugugucau auucccugug uacugcagcg     1500

uucacauuua ccaagguccc agcugaaaca uugcauggaa caguuacagu ggaggugcag     1560

ucugcaggga cagauggacc cugcaagguc ccagcccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaagccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3243
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 1 wild type

<400>  3243
gggagaaagc uuaccaugag ugucggaauu guuggccucc ugcugaccac agcuauggca       60

gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg      120

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      180

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      240

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      300

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      360

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      420

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      480

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      540

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      600

gaagguaugu caggugggac uuggguugau guugucuugg aacauggagg uugugucacc      660

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      720

auggcggagg uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc      780

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      840

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagc      900

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      960

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc     1020

guuaaugaca caggacauga aacugaugag aauagagcga agguugagau aacgcccaau     1080

ucaccaagag ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg     1140

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug     1200

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1260

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1320

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1380

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1440

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc     1500

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1560

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1620

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1680

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1740

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1800

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1860

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu     1920

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1980

augugguugg gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     2040

ggaguguuga ucuucuuauc cacagccguc ucugcuugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  3244
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 1 wild type

<400>  3244
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaacuuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

uccaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caggagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3245
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 1 wild type

<400>  3245
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaacuggaug uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguc ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3246
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 1 wild type

<400>  3246
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacaugacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga ccugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uccacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggua gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaac ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3247
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 1 wild type

<400>  3247
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcucccuucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accgcaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguu      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

cucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uuggcucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagccacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca guggacggga cagucacagu ggagggacag     1560

uacggaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcagacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uauuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau ugggacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucagguu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3248
<211>  2275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 1 wild type

<400>  3248
gggagaaagc uuaccauggg agcugaugcc agcaucggaa ucgucagccu ccugcugacu       60

acagucaugg cagcagagau cacuagacgc gggagugcau acuacaugua cuuggacagg      120

agcgaugcug guaaggccau uucuuucguu accacacugg gggugaacaa augccaugug      180

cagaucaugg accucgggca uaugugugac gccaccauga guuaugagug ccccaugcug      240

gacgagggag uggagccaga ugacgucgau ugcuggugca acacgacauc aacuuggguu      300

guguacggaa ccugucauca uaaaaaaggu gaagcacgac gauccagaag agccgugacg      360

cuuccuucuc acucuacaag gaaguugcaa acgcgaucgc agacuuggcu agaaucaaga      420

gaauacacaa agcaccugau caagguugag aauuggauau ucaggaaccc cggguuugcg      480

cuaguggcug uagcuauugc cuggcuccug ggaagcucga cgagccaaaa agucauauac      540

uuggucauga uauuguugau ugccccggca uacaguauca ggugcauagg aguuagcaau      600

agagacuucg uggagggcau gucagguggg accuggguug auguugucuu ggaacaugga      660

gguuguguca ccgugauggc acaggacaag ccaacaguug acauagaguu ggucacgaca      720

acgguuagca acauggccga ggugagaucc uacugcuacg aggcaucaau aucggacaug      780

gcuucggaca gucgcugccc aacacaaggu gaagccuacc uugacaagca gucagacacu      840

caauaugucu guaaaagaac auugguggac agagguuggg gaaaugggug uggacuuuuu      900

ggcaagggga gcuuggugac gugugccaag uuuacaugcu ccaagaaaau gacagggaag      960

agcauccagc cggagaacuu ggaguaccgg auaaugcuau cagugcaugg aucccagcac     1020

agugggauga uugugaauga cgaaaacaga gcaaaagucg agguuacacc caauucacca     1080

agagcagaag caaccuuggg agguuuugga agccugggac uugauuguga accaaggaca     1140

ggccuugacu uuucagaucu guauuaccug accaugaaca auaagcauug guuggugcac     1200

aaagaguggu uucaugacau cccauuaccu uggcauucug gugcagacac ugaaacucca     1260

cacuggaaca acaaagaggc acugguggag uucaaggacg cccacgccaa gaggcaaacu     1320

guugugguuc uggggagcca agaaggagcc guucacacgg cucucgcugg agcucuggag     1380

gcugagaugg auggugcgaa gggaaggcua uccucaggcc auuugaaaug ccgccuaaaa     1440

auggacaagc uuagguugaa ggguguguca uauucccugu guaccgcagc guucacauuc     1500

accaagguuc cagcugaaac auugcaugga acagucacag uggaggugca guaugcaggg     1560

agggauggac ccugcaaggu cccagcccag auggcggugg acaugcagac ccugacccca     1620

guuggaaggc ugauaacggc uaacccugug aucacugaaa gcacugagaa uucaaagaug     1680

auguuggagc ucgacccacc auuuggggau ucuuacauug ucauaggagu cggggacaag     1740

aaaaucaccc aucacuggca ucggaguggu agcaucaucg gaaaggcauu ugaagccacu     1800

gugagaggcg ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

gggggugugu uuaacucauu gggcaagggu auucaccaga ucuuuggagc agcuuucaaa     1920

ucacuguucg gaggaauguc cugguucuca cagauccuca uaggcacacu guuggugugg     1980

uugggucuga acacaaagaa uggaucuauc ucccucacau gcuuggccuu gggaggagug     2040

augaucuucc uuuccacggc uguuucugcu ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2220

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2275


<210>  3249
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 1 wild type

<400>  3249
gggagaaagc uuaccauggg cgcagacacc agcaucggaa ucauuggccu ccugcugacu       60

acagccaugg cagcagagau cacuagacgc gggagugcau acuacaugua cuuggauagg      120

agcgaugccg ggaaggccau uucguuugcu accacaaugg gagugaacaa gugccacgua      180

cagaucaugg accucgggca caugugugac gccaccauga guuaugagug cccuaugcug      240

gaugagggag uggaaccaga ugaugucgau ugcuggugca acacgacauc aacuuggguu      300

guguacggaa ccugucauca caaaaaaggu gaggcacggc ccucaagaag agcugucacg      360

cuuccuuccc auuccacaag aaaguuacag acacggucac aaaccuggcu ugaaucacgg      420

gaguacacga agcaccugau caaaguugag aacuggauac uccggaaucc aggguucaca      480

guugcugcuc uagcaauggc cuggcucuug ggaaauucca cgagccagcg agugaucuau      540

cuggugauga uccuguugau ugccccggca uacaguauca ggugcauugg agucagcaau      600

agagacuucg uggagggcau gucagguggg accuggguug auguugucuu ggaacaugga      660

ggcugcguua ccgugauggc acaggacaag ccaacagucg acauagaguu ggucacgacg      720

acgguuagua acauggccga gguaagaucc uauugcuacg aggcaucgau aucggacaug      780

gcuucggaca gucguugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac auuaguggac agagguuggg gaaacgguug uggacuuuuu      900

ggcaaaggga gcuuggugac augugccaag uuuacguguu cuaagaagau gaccgggaag      960

agcauucaac cggagaauuu ggaguacaga auuaugcugu cagugcacgg cucccaacac     1020

agcgggauga uugugaauga cauaggacau gagaccgaug aaaacagagc aaaaguggag     1080

gucacaccaa acucaccaag agcugaagcg acccuagggg gcuucgggag uuugggacuc     1140

gauugugagc caaggaccgg uuuagauuuc uccgaccugu acuauuuaac caugaacaac     1200

aagcauuggu uggugcacaa ggagugguuc cacgacauuc cauugccaug gcacgcugga     1260

gcggacacag ggacucccca uuggaacaac aaagaggcau ugguugaguu caaggaugcc     1320

cacgccaaaa gacagacugu ugucguguug ggaagucagg agggugccgu ccacacagcc     1380

cuggcaggag cuuuggaagc ggagauggau ggagccaaag gaaggcuguu cucaggucau     1440

uugaagugcc ggauaaagau ggacaagcuu agguugaagg gugucucaua cucauugugc     1500

acugccgccu ucacauucuc aaagguuccg gcugaaacgc ugcaugggac agucacaguu     1560

gaggugcagu augcgggcag agauggaccu ugcaaggugc cagcccaaau ggcaguggau     1620

augcagacuc uuaccccagu gggaagguug auaacggcaa aucccaucau aacugagagc     1680

acugaaaacu cgagaaugau guuggagcug gacccacccu uuggagacuc uuacauuguc     1740

auugggguug gagauaagaa aauuacccau cauuggcaca gaaguggaag cacgauuggg     1800

aaggcauuug aggccacugu gagaggugca aaaaggaugg caguccuggg agauaccgcc     1860

ugggauuuug guucuguugg aggcguguuc aauucucugg gcaagggagu ucaucagguc     1920

uucggagcag ccuuuaaauc cuuauuuggc ggaauguccu gguuuucaca aauucuuauu     1980

ggcacacuuu uagugugguu gggcuugaau acaaaaaaug gauccauuuc ccuugcaugu     2040

cuggcgcuug gaggggcgau gaucuuucuc uccacggccg ucucggcaug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3250
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 1 wild type

<400>  3250
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3251
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 1 wild type

<400>  3251
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3252
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 1 wild type

<400>  3252
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      540

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      600

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      660

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      720

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag      780

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc      840

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca      900

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug      960

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1020

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1080

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1140

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1200

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1260

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1320

auggcagucc ugggggauac agccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3253
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 1 wild type

<400>  3253
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3254
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 1 wild type

<400>  3254
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugu caaugauaca      540

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg      600

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu      660

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag      720

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc      840

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag      900

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac      960

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1020

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1080

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1140

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1200

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1260

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1320

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3255
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 1 wild type

<400>  3255
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugacugu caaugauaua      540

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg      600

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu      660

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag      720

ugguuucaug acaucccauu gccuuggcau gcuggggcag acacuggaac uccacacugg      780

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc      840

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag      900

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac      960

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1020

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1080

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1140

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1200

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1260

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1320

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3256
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 1 wild type

<400>  3256
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauaggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugacgu uguccuggaa cauggaggcu gcgucaccgu gauggcacag      180

gacaagccaa caguugacau agagcugguc acaacaacgg uuaguaacau ggccgaggug      240

agauccuauu guuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacauug      360

guggacagag guuggggaaa ugggugugga cucuuuggca aagggaguuu ggugacaugu      420

gcuaaguuca cgugcuccaa gaagaugacu gggaagagca uucagccgga gaaccuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcacagug ggaugauugu uaaugaugaa      540

aacagagcga aggucgaggu uacgcccaau ucaccaagag cagaagcaac ccugggaggc      600

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau      660

uaccuaacca ugaauaacaa gcauugguug gugcacaaag agugguuuca ugacauccca      720

uugcccuggc augcuggggc agacacugga acuccacauu ggaacaacaa ggaggcauua      780

guggaauuca aggacgccca cgccaagagg caaaccgucg ugguuuuggg gagccaggaa      840

ggagccgucc acacggcucu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga      900

aggcuauucu cuggccacuu gaaaugucgc uuaaaaaugg acaagcuuag auugaagggc      960

gugucauauu ccuugugcac cgcggcauuc acauucacca aggucccggc ugaaacacua     1020

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1080

gcccagaugg cgguggacau gcagaccuug accccagucg gaaggcugau aaccgccaac     1140

cccgugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gauaagaaaa ucacccauca cuggcauagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggcgcuaa gagaauggca     1320

guccuggggg acacagcuug ggacuuugga ucaugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  3257
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 1 wild type

<400>  3257
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaccggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      540

gacgaaaaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      600

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      660

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      720

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag      780

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc      840

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca      900

aagggaaagc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug      960

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1020

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1080

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1140

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1200

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1260

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1320

auggcagucc ugggggauac agccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3258
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 1 wild type

<400>  3258
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gccucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cugacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3259
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 1 wild type

<400>  3259
gggagaaagc uuaccaugag caaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3260
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 1 wild type

<400>  3260
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu gguacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3261
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 1 wild type

<400>  3261
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaaa gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3262
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 1 wild type

<400>  3262
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaauaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacacgc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3263
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 1 wild type

<400>  3263
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggagaua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu ggaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3264
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 1 wild type

<400>  3264
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacuccaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccagga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3265
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 1 wild type

<400>  3265
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacaugguuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuucucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3266
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 1 wild type

<400>  3266
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccu ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcauug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3267
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 1 wild type

<400>  3267
gggagaaagc uuaccaugag ccaaaaaguc auauauuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacucagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gaaucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3268
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 1 wild type

<400>  3268
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

cuggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa uguugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ccagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3269
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 1 wild type

<400>  3269
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggggguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagcuua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga cucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3270
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 1 wild type

<400>  3270
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguucuagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3271
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 1 wild type

<400>  3271
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagcuua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gaagcacaug cuaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3272
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 1 wild type

<400>  3272
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccgccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3273
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 1 wild type

<400>  3273
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu ucaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aacaauauca gacauagcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugccgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3274
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 1 wild type

<400>  3274
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucuccaag     1020

aucccggcug aaacacugca cgggacaguc acagaggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc gguaaucacu gaaagcacag agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3275
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 1 wild type

<400>  3275
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cugacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accgagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagu uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3276
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 1 wild type

<400>  3276
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaauc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucagu augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaauccua ggacuggauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3277
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 1 wild type

<400>  3277
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug uaaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa aaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuuaggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3278
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 1 wild type

<400>  3278
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguug      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc aggggcagau     1080

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3279
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 1 wild type

<400>  3279
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc aguaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aacaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac acuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaggcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3280
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 1 wild type

<400>  3280
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgc aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugcucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggc ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag ccaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacagugga cgggacaguc acaguggagg gacaguacgg agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc agacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3281
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 1 wild type

<400>  3281
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      180

gacaaaccgg cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuauu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcu      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcau gcuggggcag acaccggaac uccacauugg      780

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

aggcugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaucacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3282
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 1 wild type

<400>  3282
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaua gcaucaggug cauaggaguc aacaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu cgucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcagaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gccaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccgaauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gaugcacaug ccaaaaggca aacugucgug      840

guccuaggga gucaagaggg agcaguucac acggcucuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagac ugaagggcgu gucauacucc cuguguaccg cagcguucac auucacuaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3283
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 1 wild type

<400>  3283
gggagaaagc uuaccaugag ccaaaaaguc auauauuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggaggcu guguuaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag uuagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gauauggcuu cggacagccg cugcccaaca      300

caaggugagg ccuaccuuga caagcaguca gacacucaau augucugcaa aagaacguua      360

guggauagag gcuggggaaa uggaugugga cuuuuuggaa aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaccuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua ccugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcau gcuggggcag acacuggaac uccacauugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacucagau ugaagggugu gucauacucc uugugugcug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc uguaaucacu gaaagcaccg agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3284
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 1 wild type

<400>  3284
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu guugauugcc       60

ccggcauaca guaucaggug cauaggaguc agcaauaggg auuuugugga agguauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      180

gacaagccaa cuguugauau agaguugguc acaacaacgg uuagcaacau ggcggaggua      240

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau auguuugcaa aagaacguua      360

guggacagag guuggggaaa uggaugugga cucuuuggca aagggagccu ggugacaugc      420

gccaaguuug caugcuccaa gaaaaugacu gggaagagca uccagccaga gaaccuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugauugu uaaugacaua      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu gcacaaggag      720

ugguuccaug acauuccacu accuuggcau gcuggggcag acaccggaac uccacauugg      780

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agccguucac acggcucuug cuggagcccu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgcuu gaaaauggac      960

aaacuuagau ugaagggcgu gucauacucc uuauguaccg cggcguucac auucaccaag     1020

aucccggcug aaacgcugca ugggacaguc acaguggagg uacaguaugc agggacagau     1080

ggacccugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc ugugaucacu gaaagcacug agaauucaaa gaugauguug     1200

gaacuugacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc aucggaaaag cauuugaagc cacugugaga     1320

ggcgccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3285
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 1 wild type

<400>  3285
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu guugauugcc       60

ccggcauaca guaucaggug cauaggaguc agcaauaggg auuuugugga agguauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      180

gacaagccaa cuguugauau agaguugguc acaacaacgg uuagcaacau ggcggaggua      240

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau auguuugcaa aagaacguua      360

guggacagag guuggggaaa uggaugugga cucuuuggca aagggagccu ggugacaugc      420

gccaaguuug caugcuccaa gaaaaugacu gggaagagca uccagccaga gaaccuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugauugu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu gcacaaggag      720

ugguuccaug acauuccacu accuuggcau gcuggggcag acaccggaac uccacauugg      780

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agccguucac acggcucuug cuggagcccu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgcuu gaaaauggac      960

aaacuuagau ugaagggcgu gucauacucc uuauguaccg cggcguucac auucaccaag     1020

aucccggcug aaacgcugca ugggacaguc acaguggagg uacaguaugc agggacagau     1080

ggacccugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc ugugaucacu gaaagcacug agaauucaaa gaugauguug     1200

gaacuugacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaagauc     1260

acccaccacu gguacaggag uggcagcacc aucggaaaag cauuugaagc cacugugaga     1320

ggcgccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3286
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 1 wild type

<400>  3286
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauaggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugacgu uguccuggaa cauggaggcu gcgucaccgu gauggcacag      180

gacaagccaa caguugacau agagcugguc acaacaacgg uuaguaacau ggccgaggug      240

agauccuauu guuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacauug      360

guggacagag guuggggaaa ugggugugga cucuuuggca aagggaguuu ggugacaugu      420

gcuaaguuca cgugcuccaa gaagaugacu gggaagagca uucagccgga gaaccuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcacagug ggaugauugu uaaugacaca      540

ggacaugaaa cugaugaaaa cagagcgaag gucgagguua cgcccaauuc accaagagca      600

gaagcaaccc ugggaggcuu uggaagcuua ggacuugauu gugaaccaag gacaggccuu      660

gacuuuucag aucuguauua ccuaaccaug aauaacaagc auugguuggu gcacaaagag      720

ugguuucaug acaucccauu gcccuggcau gcuggggcag acacuggaac uccacauugg      780

aacaacaagg aggcauuagu ggaauucaag gacgcccacg ccaagaggca aaccgucgug      840

guuuugggga gccaggaagg agccguccac acggcucuug cuggagcucu agaggcugag      900

auggauggug caaagggaag gcuauucucu ggccacuuga aaugucgcuu aaaaauggac      960

aagcuuagau ugaagggcgu gucauauucc uugugcaccg cggcauucac auucaccaag     1020

gucccggcug aaacacuaca uggaacaguc acaguggagg ugcaguaugc agggacagau     1080

ggacccugca aggucccagc ccagauggcg guggacaugc agaccuugac cccagucgga     1140

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaauucaaa gaugauguug     1200

gagcucgacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaaaauc     1260

acccaucacu ggcauaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggcgcuaaga gaauggcagu ccugggggac acagcuuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3287
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 1 wild type

<400>  3287
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg uuaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauuggaguc agcaauagag acuucgugga aggcauguca      120

ggugggaccu ggguugaugu cgucuuggaa cauggagguu gcgucaccgu uauggcacag      180

gacaagccaa caguugacau agaguugguc acgacaacgg uuaguaacau ggccgaggua      240

agauccuacu guuaugaggc aucaauauca gacauggcuu cggacagucg cugcccaaca      300

caaggugaag ccuaccuuga uaagcaauca gacacccaau augugugcaa aagaacauug      360

guggacagag guuggggaaa ugggugugga cuuuuuggca aagggagccu ggugacaugu      420

gccaaguuca cguguuccaa gaagaugacu gggaagagca uucagccgga gaaucuggag      480

uaucgaauaa ugcuaucagu gcauggcucc cagcacagcg ggaugauugu uaaugacaua      540

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguca cgcccaauuc accaagagca      600

gaagcaaccu ugggaggcuu uggaagcuua ggacuugauu gugaaccaag gacaggccug      660

gacuuuucag auuuguacua cuugaccaug aacaacaagc acugguuggu gcacaaagag      720

ugguuucaug acaucccauu gccuuggcau gcuggggcag auaccggaac uccacacugg      780

aacaacaagg aggcauuggu agaguucaaa gaugcccacg ccaagaggca aaccgucguu      840

guucuaggga gccaggaagg agccguucac acggcucuug cuggagcucu ggaggcugag      900

auggauggug caaaaggaag gcuguucucc ggccauuuga aaugccgcuu aaaaauggac      960

aagcuuagau ugaagggcgu gucauauucc uugugcaccg cagcguucac auuuaccaag     1020

gucccagcug aaacauugca uggaacaguu acaguggagg ugcaguaugc agggacagau     1080

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccagucggg     1140

aggcugauaa ccgccaaccc ugugauuacu gaaagcacug agaauucaaa gaugauguug     1200

gagcucgacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1260

acccaucacu ggcauaggag ugguagcacc aucggaaaag cauuugaggc cacugugaga     1320

ggugccaaga gaauggcagu ucugggggau acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3288
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 1 wild type

<400>  3288
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg uaaugauauu guugauugcc       60

ccggcauaca gcaucaggug cauaggaguu agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggggguu gcgucaccgu gauggcacag      180

gacaagccaa caguugacau cgaguugguc acgacaacgg uuagcaacau ggccgaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cuguccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa gagaacauug      360

guggauagag guuggggaaa ugggugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cgugcuccaa gaaaaugaca ggcaagagca uccagccgga gaacuuggag      480

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacaua      540

ggacaugaaa cugacgaaaa cagagcaaaa gucgagguca cacccaauuc accaagagca      600

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu      660

gacuucucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaggag      720

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg      780

aacaacaaag aggcauuggu ggaguuuaag gacgcccacg ccaagaggca aacuguugug      840

guucugggga gccaagaggg agcuguucac acggcccucg cuggagcuuu ggaggcugag      900

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac      960

aagcuuaggu ugaagggugu gucauauucc cuguguaccg caguguguac cgcagcgaag     1020

gucccagcug aaacauugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1080

ggacccugca aggucccagc ccagauggcg guagacaugc agacccugac cccaguugga     1140

aggcugauaa ccgccaaccc ugugaucacu gaaaguacug agaauucaaa gaugauguug     1200

gagcucgacc caccauuugg ggauucuuac auugucauag gagucgggga caagaaaauc     1260

acccaucacu ggcaucggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggggac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3289
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 1 wild type

<400>  3289
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauauu guugauugcc       60

ccggcauaca guaucaggug cauaggaguu agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggagguu gugucaccgu gauggcacag      180

gacaagccaa caguugacau agaguugguc acgacaacgg uuagcaacau ggccgaggug      240

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau augucuguaa aagaacauug      360

guggacagag guuggggaaa ugggugugga cuuuuuggca aggggagcuu ggugacgugu      420

gccaaguuua caugcuccaa gaaaaugaca gggaagagca uccagccgga gaacuuggag      480

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacgaa      540

aacagagcaa aagucgaggu uacacccaau ucaccaagag cagaagcaac cuugggaggu      600

uuuggaagcc ugggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau      660

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca      720

uuaccuuggc auucuggugc agacacugaa acuccacacu ggaacaacaa agaggcacug      780

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagaa      840

ggagccguuc acacggcucu cgcuggagcu cuggaggcug agauggaugg ugcgaaggga      900

aggcuauccu caggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu      960

gugucauauu cccuguguac cgcagcguuc acauucacca agguuccagc ugaaacauug     1020

cauggaacag ucacagugga ggugcaguau gcagggaggg auggacccug caagguccca     1080

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aacggcuaac     1140

ccugugauca cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1200

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1260

agugguagca ucaucggaaa ggcauuugaa gccacuguga gaggcgccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga ucaugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  3290
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 1 wild type

<400>  3290
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauagu guugauuguc       60

ccggcauaca guaucagcug cauuggaguc agcaauagag acuuagugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggagggu gcguuaccga gauggcacag      180

gacaagccaa caguugacau agaguugguc acgaugacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucguuaucc gacauggcuu cggccagucg uugcccaaca      300

caaggcgaac ccucccucga caagcaauca gacacucaau cuguaugcaa aagaacauua      360

ggagacagag guuggggaaa ugguuguggg auuuuuggca aagggagcuu ggugacaugu      420

uccaaguuca cguguuguaa gaagaugccc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcucccagu gcauggcucc cagcauagcg ggaugauugu gaaugacaua      540

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguca cacccaauuc accaagagca      600

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu      660

gacuucucag aucuguauua ucugaccaug aacaacaagc auugguuggu gcacaaggag      720

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg      780

aacaacaaag aggcauuggu ggaguucaag gacgcccacg ccaagaggca aacuguugug      840

guucugggga gccaagaggg agcuguucac acggcccucg cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac      960

aagcuuaggu ugaagggugu gucauauucc cuguguacug cagcguucac auuuaccaag     1020

gucccagcug aaacauugca uggaacaguu acaguggagg ugcagucugc agggacagau     1080

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccaguugga     1140

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1200

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1260

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1320

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3291
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 1 wild type

<400>  3291
gggagaaagc uuaccaugcg ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu agaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacugau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacuccuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3292
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 1 wild type

<400>  3292
gggagaaagc uuaccaugag ccagcgagug aucuaucugg ugaugauccu guugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga gaauuuggag      480

uacagaauua ugcugucagu gcacggcucc caacacagcg ggaugauugu gaaugacaua      540

ggacaugaga ccgaugaaaa cagagcaaaa guggagguca caccaaacuc accaagagcu      600

gaagcgaccc uagggggcuu cgggaguuug ggacucgauu gugagccaag gaccgguuua      660

gauuucuccg accuguacua uuuaaccaug aacaacaagc auugguuggu gcacaaggag      720

ugguuccacg acauuccauu gccauggcac gcuggagcgg acacagggac uccccauugg      780

aacaacaaag aggcauuggu ugaguucaag gaugcccacg ccaaaagaca gacuguuguc      840

guguugggaa gucaggaggg ugccguccac acagcccugg caggagcuuu ggaagcggag      900

auggauggag ccaaaggaag gcuguucuca ggucauuuga agugccggau aaagauggac      960

aagcuuaggu ugaagggugu cucauacuca uugugcacug ccgccuucac auucucaaag     1020

guuccggcug aaacgcugca ugggacaguc acaguugagg ugcaguaugc gggcagagau     1080

ggaccuugca aggugccagc ccaaauggca guggauaugc agacucuuac cccaguggga     1140

agguugauaa cggcaaaucc caucauaacu gagagcacug aaaacucgag aaugauguug     1200

gagcuggacc cacccuuugg agacucuuac auugucauug ggguuggaga uaagaaaauu     1260

acccaucauu ggcacagaag uggaagcacg auugggaagg cauuugaggc cacugugaga     1320

ggugcaaaaa ggauggcagu ccugggagau accgccuggg auuuugguuc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3293
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4804/R4834 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 1 GC

<400>  3293
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3294
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4816/R4838 ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA 
       Design 1 GC

<400>  3294
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacacg      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac cacgagcacc ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacgcgcaag      420

cugcagaccc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccacgag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg     1080

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc     1380

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cccugcugau guggcucggg     2040

cugaacgcca agaacggcuc caucagccug augugccucg cgcugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3295
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4820/R4842 ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 
       1 GC

<400>  3295
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agcggaagcg ccggggcgcc       60

gacaccagca ucgggaucau cggccugcug cucacgaccg ccauggcggc cgagaucacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      180

uucgcgacca cgcugggcgu gaacaagugc cacgugcaga ucauggaccu cgggcacaug      240

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccugc ccucccacag cacccggaag      420

cuccagacgc gcucccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      480

guggagaacu ggaucuuccg gaaccccggc uucgcccucg ucgcgguggc caucgccugg      540

cugcuggggu ccagcaccuc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc      600

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      660

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      840

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      900

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      960

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg     1080

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug     1140

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac     1200

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc     1380

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg     1440

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug     1500

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1620

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1680

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1740

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1860

auggccgugc ucggggacac cgccugggac uucggcagcg ugggcggcgu cuucaacucc     1920

cuggggaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucgggacg cugcuggugu ggcucggccu gaacaccaag     2040

aacggcucca ucagccugac cugccucgcg cugggcggcg ugaugaucuu ccuguccacc     2100

gccgucagcg ccgacguggg gugcuccgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3296
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4868/R5082 ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA 
       Design 1 GC

<400>  3296
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc     1380

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgcggugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3297
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4880/R5081 ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA 
       Design 1 GC

<400>  3297
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3298
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 1 GC

<400>  3298
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3299
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 1 GC

<400>  3299
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccgaga ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguu cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcga agcggauggc cgugcucggg gacaccgccu gggacuucgg cagcgugggc     1860

ggcgucuuca acucccuggg gaaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg ggacgcugcu gguguggcuc     1980

ggccugaaca ccaagaacgg cuccaucagc cugaccugcc ucgcgcuggg cggcgugaug     2040

aucuuccugu ccaccgccgu cagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3300
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4870/R5083 ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA 
       Design 1 GC

<400>  3300
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3301
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4826/R4846 ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA 
       Design 1 GC

<400>  3301
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      180

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      360

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug      840

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1080

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1140

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3302
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4825/R4845 ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA 
       Design 1 GC

<400>  3302
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3303
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 GC

<400>  3303
gggagaaagc uuaccauguc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc       60

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      120

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      180

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      300

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      420

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg      540

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug      600

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac      660

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc      840

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg      900

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug      960

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1080

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1140

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1200

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1260

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1320

auggccgugc ucggggacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3304
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 1 GC

<400>  3304
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc      840

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3305
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       1 GC

<400>  3305
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuc cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3306
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 1
       GC

<400>  3306
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuc cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3307
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 1 GC

<400>  3307
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccgaga ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguc cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcga agcggauggc cgugcucggg gacaccgccu gggacuucgg cagcgugggc     1860

ggcgucuuca acucccuggg gaaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg ggacgcugcu gguguggcuc     1980

ggccugaaca ccaagaacgg cuccaucagc cugaccugcc ucgcgcuggg cggcgugaug     2040

aucuuccugu ccaccgccgu cagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3308
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 1 
       GC

<400>  3308
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuc cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3309
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       1 GC

<400>  3309
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucguccagg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3310
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 1
       GC

<400>  3310
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugcagg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3311
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 1 
       GC

<400>  3311
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugcagg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3312
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 GC

<400>  3312
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3313
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 GC

<400>  3313
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3314
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 1 GC

<400>  3314
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccgaga ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguu cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc     1800

aagggcgccc agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg     1860

ggcguguuca acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc     1920

cucuucgggg gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug     1980

ggggugaacg cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug     2040

guguuccugg ccaccaacgu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3315
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 GC

<400>  3315
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3316
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5620 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1
       GC

<400>  3316
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3317
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3317
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcggcg cggcgccgac       60

accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg      120

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      180

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      240

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      360

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      420

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      480

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      540

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      600

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      660

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      720

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      780

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      840

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      900

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac     1020

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1080

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1140

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1200

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1380

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1440

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1500

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1620

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1680

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1740

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1800

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1860

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1920

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     2040

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2100

cucuccaccg cggugagcgc cgacgugggg ugcuccgucg acuucagcaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  3318
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3318
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcggcgcgg cgccgacacc       60

agcgugggga ucgugggccu ccugcugacg accgccaugg cggccgaggu cacccggcgc      120

ggcagcgccu acuacaugua ccucgaccgg aacgacgccg gggaggccau cagcuucccg      180

accacgcugg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      240

gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag cacguggguc guguacggca cgugccacca caagaagggg      360

gaggcccgcc ggucccgccg ggccgugacc cugcccagcc acuccacccg caagcugcag      420

acgcggagcc agaccuggcu cgagucccgc gaguacacca agcaccugau ccgggucgag      480

aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg ccgccaucgc cuggcuccug      540

gggagcucca ccagccagaa ggugaucuac cuggugauga uccuccugau cgcccccgcg      600

uacuccaucc gcugcaucgg cgucagcaac cgggacuucg uggaggggau guccggcggc      660

acgugggugg acaucguccu ggagcacggg ggcugcguga ccgugauggc ccaggacaag      720

cccaccgucg acaucgagcu cgugaccacg accgugagca acauggccga gguccgcagc      780

uacugcuacg aggccuccau cagcgacaug gccuccgaca gccggugccc cacccagggg      840

gaggcguacc uggacaagca guccgacacc caguacgugu gcaagcgcac gcuggucgac      900

cggggcuggg ggaacggcug cggccucuuc gggaagggca gccuggugac cugcgccaag      960

uucgccugcu ccaagaagau gaccgggaag uccauccagc cggagaaccu ggaguaccgc     1020

aucaugcucu ccgugcacgg cagccagcac uccgggauga ucgucaacga caccggccac     1080

gagacggacg agaaccgggc caagguggag aucaccccca acagcccccg cgcggaggcc     1140

acccugggcg gguucggcuc ccuggggcuc gacugcgagc cccggaccgg ccuggacuuc     1200

agcgaccugu acuaccucac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cgcugcccug gcacgccggc gccgacaccg ggaccccgca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcg cacgccaagc gccagaccgu ggugguccug     1380

ggcucccagg agggggccgu gcacacggcc cuggccggcg cgcuggaggc cgagauggac     1440

ggggccaagg gccggcucag cuccggccac cugaagugcc gccugaagau ggacaagcuc     1500

cggcugaagg gggugagcua cucccugugc accgccgccu ucaccuucac caagaucccc     1560

gcggagaccc uccacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccg     1620

ugcaaggucc ccgcccagau ggccguggac augcagaccc ugacgcccgu ggggcgccug     1680

aucaccgcga accccgucau caccgagagc accgagaaca gcaagaugau gcucgagcug     1740

gacccgcccu ucggcgacuc cuacaucgug aucggcgugg gggagaagaa gaucacgcac     1800

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg gcagcguggg cggggcccuc     1920

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1980

ggcauguccu gguucagcca gauccucauc ggcacgcugc ugauguggcu cgggcugaac     2040

accaagaacg gcuccaucag ccugaugugc cucgcccugg gcggcguccu gaucuuccuc     2100

uccaccgcgg ugagcgccga cguggggugc uccgucgacu ucagcaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  3319
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3319
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc ggcgcggcgc cgacaccagc       60

guggggaucg ugggccuccu gcugacgacc gccauggcgg ccgaggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3320
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3320
gggagaaagc uuaccauggc ccgcaaggag aagaagcggc gcggcgccga caccagcgug       60

gggaucgugg gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc      120

gccuacuaca uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg      180

cugggcauga acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc      240

auguccuacg agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc      360

cgccgguccc gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      420

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      480

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      540

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      600

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      660

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      720

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      780

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      840

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      900

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug     1020

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg     1080

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug     1140

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac     1200

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1380

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1440

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1500

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1560

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1620

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1680

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1740

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1920

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     2040

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     2100

gcggugagcg ccgacguggg gugcuccguc gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3321
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3321
gggagaaagc uuaccaugcg caaggagaag aagcggcgcg gcgccgacac cagcgugggg       60

aucgugggcc uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc      120

uacuacaugu accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug      180

ggcaugaaca agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug      240

uccuacgagu gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc      360

cggucccgcc gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      480

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      540

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      600

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      660

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      780

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      840

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      900

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      960

uccaagaaga ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc     1020

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac     1080

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc     1140

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug     1200

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1380

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1440

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1500

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1560

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1620

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1680

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1740

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1800

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1860

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1920

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1980

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     2040

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     2100

gugagcgccg acguggggug cuccgucgac uucagcaagu gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  3322
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3322
gggagaaagc uuaccaugaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3323
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3323
gggagaaagc uuaccaugga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgacgugg ggugcuccgu cgacuucagc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  3324
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3324
gggagaaagc uuaccaugaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gacguggggu gcuccgucga cuucagcaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  3325
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3325
gggagaaagc uuaccaugaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu ccgucgacuu cagcaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3326
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3326
gggagaaagc uuaccaugcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacgug     2100

gggugcuccg ucgacuucag caagugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3327
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3327
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcuccgucg acuucagcaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3328
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3328
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cguggggugc     2100

uccgucgacu ucagcaagug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3329
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3329
gggagaaagc uuaccauggc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugcucc     2100

gucgacuuca gcaagugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3330
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3330
gggagaaagc uuaccaugga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcuccguc     2100

gacuucagca agugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3331
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3331
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cuccgucgac     2100

uucagcaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3332
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3332
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgcag ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cuccgucgac     2100

uucagcaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3333
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3333
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccgc agucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcuccgucg acuucagcaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3334
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3334
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcggggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3335
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3335
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcgcagucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3336
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3336
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3337
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3337
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcggcg cggcgccgac       60

accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg      120

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      180

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      240

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      360

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      420

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      480

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      540

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      600

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      660

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      720

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      780

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      840

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      900

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac     1020

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1080

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1140

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1200

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1380

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1440

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1500

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1620

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1680

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1740

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1800

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1860

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1920

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     2040

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2100

cucuccaccg cggugagcgc cgacgugggg ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  3338
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3338
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcggcgcgg cgccgacacc       60

agcgugggga ucgugggccu ccugcugacg accgccaugg cggccgaggu cacccggcgc      120

ggcagcgccu acuacaugua ccucgaccgg aacgacgccg gggaggccau cagcuucccg      180

accacgcugg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      240

gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag cacguggguc guguacggca cgugccacca caagaagggg      360

gaggcccgcc ggucccgccg ggccgugacc cugcccagcc acuccacccg caagcugcag      420

acgcggagcc agaccuggcu cgagucccgc gaguacacca agcaccugau ccgggucgag      480

aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg ccgccaucgc cuggcuccug      540

gggagcucca ccagccagaa ggugaucuac cuggugauga uccuccugau cgcccccgcg      600

uacuccaucc gcugcaucgg cgucagcaac cgggacuucg uggaggggau guccggcggc      660

acgugggugg acaucguccu ggagcacggg ggcugcguga ccgugauggc ccaggacaag      720

cccaccgucg acaucgagcu cgugaccacg accgugagca acauggccga gguccgcagc      780

uacugcuacg aggccuccau cagcgacaug gccuccgaca gccggugccc cacccagggg      840

gaggcguacc uggacaagca guccgacacc caguacgugu gcaagcgcac gcuggucgac      900

cggggcuggg ggaacggcug cggccucuuc gggaagggca gccuggugac cugcgccaag      960

uucgccugcu ccaagaagau gaccgggaag uccauccagc cggagaaccu ggaguaccgc     1020

aucaugcucu ccgugcacgg cagccagcac uccgggauga ucgucaacga caccggccac     1080

gagacggacg agaaccgggc caagguggag aucaccccca acagcccccg cgcggaggcc     1140

acccugggcg gguucggcuc ccuggggcuc gacugcgagc cccggaccgg ccuggacuuc     1200

agcgaccugu acuaccucac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cgcugcccug gcacgccggc gccgacaccg ggaccccgca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcg cacgccaagc gccagaccgu ggugguccug     1380

ggcucccagg agggggccgu gcacacggcc cuggccggcg cgcuggaggc cgagauggac     1440

ggggccaagg gccggcucag cuccggccac cugaagugcc gccugaagau ggacaagcuc     1500

cggcugaagg gggugagcua cucccugugc accgccgccu ucaccuucac caagaucccc     1560

gcggagaccc uccacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccg     1620

ugcaaggucc ccgcccagau ggccguggac augcagaccc ugacgcccgu ggggcgccug     1680

aucaccgcga accccgucau caccgagagc accgagaaca gcaagaugau gcucgagcug     1740

gacccgcccu ucggcgacuc cuacaucgug aucggcgugg gggagaagaa gaucacgcac     1800

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg gcagcguggg cggggcccuc     1920

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1980

ggcauguccu gguucagcca gauccucauc ggcacgcugc ugauguggcu cgggcugaac     2040

accaagaacg gcuccaucag ccugaugugc cucgcccugg gcggcguccu gaucuuccuc     2100

uccaccgcgg ugagcgccga cguggggugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  3339
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3339
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc ggcgcggcgc cgacaccagc       60

guggggaucg ugggccuccu gcugacgacc gccauggcgg ccgaggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3340
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3340
gggagaaagc uuaccauggc ccgcaaggag aagaagcggc gcggcgccga caccagcgug       60

gggaucgugg gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc      120

gccuacuaca uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg      180

cugggcauga acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc      240

auguccuacg agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc      360

cgccgguccc gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      420

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      480

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      540

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      600

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      660

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      720

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      780

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      840

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      900

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug     1020

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg     1080

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug     1140

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac     1200

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1380

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1440

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1500

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1560

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1620

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1680

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1740

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1920

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     2040

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     2100

gcggugagcg ccgacguggg gugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3341
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3341
gggagaaagc uuaccaugcg caaggagaag aagcggcgcg gcgccgacac cagcgugggg       60

aucgugggcc uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc      120

uacuacaugu accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug      180

ggcaugaaca agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug      240

uccuacgagu gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc      360

cggucccgcc gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      480

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      540

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      600

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      660

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      780

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      840

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      900

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      960

uccaagaaga ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc     1020

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac     1080

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc     1140

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug     1200

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1380

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1440

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1500

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1560

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1620

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1680

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1740

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1800

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1860

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1920

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1980

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     2040

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     2100

gugagcgccg acguggggug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3342
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3342
gggagaaagc uuaccaugaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3343
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3343
gggagaaagc uuaccaugga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgacgugg ggugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3344
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3344
gggagaaagc uuaccaugaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gacguggggu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3345
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3345
gggagaaagc uuaccaugaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3346
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3346
gggagaaagc uuaccaugcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacgug     2100

gggugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3347
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3347
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3348
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3348
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cguggggugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3349
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3349
gggagaaagc uuaccauggc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3350
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3350
gggagaaagc uuaccaugga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3351
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3351
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3352
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3352
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgcag ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3353
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3353
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccgc agucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3354
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3354
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcggggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3355
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3355
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcgcagucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3356
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3356
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3357
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3357
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcggcg cggcgccgac       60

accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg      120

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      180

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      240

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      360

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      420

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      480

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      540

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      600

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      660

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      720

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      780

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      840

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      900

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac     1020

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1080

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1140

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1200

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1380

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1440

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1500

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1620

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1680

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1740

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1800

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1860

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1920

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     2040

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2100

cucuccaccg cggugagcgc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3358
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3358
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcggcgcgg cgccgacacc       60

agcgugggga ucgugggccu ccugcugacg accgccaugg cggccgaggu cacccggcgc      120

ggcagcgccu acuacaugua ccucgaccgg aacgacgccg gggaggccau cagcuucccg      180

accacgcugg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      240

gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag cacguggguc guguacggca cgugccacca caagaagggg      360

gaggcccgcc ggucccgccg ggccgugacc cugcccagcc acuccacccg caagcugcag      420

acgcggagcc agaccuggcu cgagucccgc gaguacacca agcaccugau ccgggucgag      480

aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg ccgccaucgc cuggcuccug      540

gggagcucca ccagccagaa ggugaucuac cuggugauga uccuccugau cgcccccgcg      600

uacuccaucc gcugcaucgg cgucagcaac cgggacuucg uggaggggau guccggcggc      660

acgugggugg acaucguccu ggagcacggg ggcugcguga ccgugauggc ccaggacaag      720

cccaccgucg acaucgagcu cgugaccacg accgugagca acauggccga gguccgcagc      780

uacugcuacg aggccuccau cagcgacaug gccuccgaca gccggugccc cacccagggg      840

gaggcguacc uggacaagca guccgacacc caguacgugu gcaagcgcac gcuggucgac      900

cggggcuggg ggaacggcug cggccucuuc gggaagggca gccuggugac cugcgccaag      960

uucgccugcu ccaagaagau gaccgggaag uccauccagc cggagaaccu ggaguaccgc     1020

aucaugcucu ccgugcacgg cagccagcac uccgggauga ucgucaacga caccggccac     1080

gagacggacg agaaccgggc caagguggag aucaccccca acagcccccg cgcggaggcc     1140

acccugggcg gguucggcuc ccuggggcuc gacugcgagc cccggaccgg ccuggacuuc     1200

agcgaccugu acuaccucac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cgcugcccug gcacgccggc gccgacaccg ggaccccgca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcg cacgccaagc gccagaccgu ggugguccug     1380

ggcucccagg agggggccgu gcacacggcc cuggccggcg cgcuggaggc cgagauggac     1440

ggggccaagg gccggcucag cuccggccac cugaagugcc gccugaagau ggacaagcuc     1500

cggcugaagg gggugagcua cucccugugc accgccgccu ucaccuucac caagaucccc     1560

gcggagaccc uccacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccg     1620

ugcaaggucc ccgcccagau ggccguggac augcagaccc ugacgcccgu ggggcgccug     1680

aucaccgcga accccgucau caccgagagc accgagaaca gcaagaugau gcucgagcug     1740

gacccgcccu ucggcgacuc cuacaucgug aucggcgugg gggagaagaa gaucacgcac     1800

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg gcagcguggg cggggcccuc     1920

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1980

ggcauguccu gguucagcca gauccucauc ggcacgcugc ugauguggcu cgggcugaac     2040

accaagaacg gcuccaucag ccugaugugc cucgcccugg gcggcguccu gaucuuccuc     2100

uccaccgcgg ugagcgccga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3359
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3359
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc ggcgcggcgc cgacaccagc       60

guggggaucg ugggccuccu gcugacgacc gccauggcgg ccgaggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3360
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3360
gggagaaagc uuaccauggc ccgcaaggag aagaagcggc gcggcgccga caccagcgug       60

gggaucgugg gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc      120

gccuacuaca uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg      180

cugggcauga acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc      240

auguccuacg agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc      360

cgccgguccc gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      420

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      480

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      540

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      600

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      660

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      720

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      780

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      840

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      900

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug     1020

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg     1080

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug     1140

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac     1200

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1380

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1440

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1500

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1560

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1620

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1680

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1740

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1920

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     2040

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     2100

gcggugagcg ccgacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3361
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3361
gggagaaagc uuaccaugcg caaggagaag aagcggcgcg gcgccgacac cagcgugggg       60

aucgugggcc uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc      120

uacuacaugu accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug      180

ggcaugaaca agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug      240

uccuacgagu gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc      360

cggucccgcc gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      480

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      540

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      600

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      660

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      780

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      840

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      900

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      960

uccaagaaga ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc     1020

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac     1080

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc     1140

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug     1200

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1380

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1440

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1500

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1560

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1620

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1680

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1740

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1800

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1860

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1920

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1980

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     2040

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     2100

gugagcgccg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3362
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3362
gggagaaagc uuaccaugaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3363
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3363
gggagaaagc uuaccaugga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3364
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3364
gggagaaagc uuaccaugaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3365
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3365
gggagaaagc uuaccaugaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3366
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3366
gggagaaagc uuaccaugcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3367
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3367
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3368
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3368
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3369
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3369
gggagaaagc uuaccauggc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3370
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3370
gggagaaagc uuaccaugga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  3371
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3371
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3372
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3372
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgcag ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3373
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3373
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccgc agucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3374
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3374
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcggggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3375
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3375
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcgcagucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3376
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3376
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3377
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3377
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcggcgcgg cgccgacacc       60

agcgugggga ucgugggccu ccugcugacg accgccaugg cggccgaggu cacccggcgc      120

ggcagcgccu acuacaugua ccucgaccgg aacgacgccg gggaggccau cagcuucccg      180

accacgcugg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      240

gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag cacguggguc guguacggca cgugccacca caagaagggg      360

gaggcccgcc ggucccgccg ggccgugacc cugcccagcc acuccacccg caagcugcag      420

acgcggagcc agaccuggcu cgagucccgc gaguacacca agcaccugau ccgggucgag      480

aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg ccgccaucgc cuggcuccug      540

gggagcucca ccagccagaa ggugaucuac cuggugauga uccuccugau cgcccccgcg      600

uacuccaucc gcugcaucgg cgucagcaac cgggacuucg uggaggggau guccggcggc      660

acgugggugg acaucguccu ggagcacggg ggcugcguga ccgugauggc ccaggacaag      720

cccaccgucg acaucgagcu cgugaccacg accgugagca acauggccga gguccgcagc      780

uacugcuacg aggccuccau cagcgacaug gccuccgaca gccggugccc cacccagggg      840

gaggcguacc uggacaagca guccgacacc caguacgugu gcaagcgcac gcuggucgac      900

cggggcuggg ggaacggcug cggccucuuc gggaagggca gccuggugac cugcgccaag      960

uucgccugcu ccaagaagau gaccgggaag uccauccagc cggagaaccu ggaguaccgc     1020

aucaugcucu ccgugcacgg cagccagcac uccgggauga ucgucaacga caccggccac     1080

gagacggacg agaaccgggc caagguggag aucaccccca acagcccccg cgcggaggcc     1140

acccugggcg gguucggcuc ccuggggcuc gacugcgagc cccggaccgg ccuggacuuc     1200

agcgaccugu acuaccucac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cgcugcccug gcacgccggc gccgacaccg ggaccccgca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcg cacgccaagc gccagaccgu ggugguccug     1380

ggcucccagg agggggccgu gcacacggcc cuggccggcg cgcuggaggc cgagauggac     1440

ggggccaagg gccggcucag cuccggccac cugaagugcc gccugaagau ggacaagcuc     1500

cggcugaagg gggugagcua cucccugugc accgccgccu ucaccuucac caagaucccc     1560

gcggagaccc uccacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccg     1620

ugcaaggucc ccgcccagau ggccguggac augcagaccc ugacgcccgu ggggcgccug     1680

aucaccgcga accccgucau caccgagagc accgagaaca gcaagaugau gcucgagcug     1740

gacccgcccu ucggcgacuc cuacaucgug aucggcgugg gggagaagaa gaucacgcac     1800

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg gcagcguggg cggggcccuc     1920

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1980

ggcauguccu gguucagcca gauccucauc ggcacgcugc ugauguggcu cgggcugaac     2040

accaagaacg gcuccaucag ccugaugugc cucgcccugg gcggcguccu gaucuuccuc     2100

uccaccgcgg ugagcgccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3378
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3378
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcggcg cggcgccgac       60

accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg      120

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      180

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      240

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      360

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      420

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      480

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      540

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      600

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      660

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      720

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      780

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      840

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      900

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac     1020

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1080

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1140

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1200

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1380

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1440

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1500

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1620

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1680

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1740

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1800

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1860

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1920

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     2040

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2100

cucuccaccg cggugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3379
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3379
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc ggcgcggcgc cgacaccagc       60

guggggaucg ugggccuccu gcugacgacc gccauggcgg ccgaggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3380
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3380
gggagaaagc uuaccauggc ccgcaaggag aagaagcggc gcggcgccga caccagcgug       60

gggaucgugg gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc      120

gccuacuaca uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg      180

cugggcauga acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc      240

auguccuacg agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc      360

cgccgguccc gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      420

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      480

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      540

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      600

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      660

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      720

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      780

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      840

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      900

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug     1020

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg     1080

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug     1140

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac     1200

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1380

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1440

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1500

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1560

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1620

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1680

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1740

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1920

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     2040

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     2100

gcggugagcg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3381
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3381
gggagaaagc uuaccaugcg caaggagaag aagcggcgcg gcgccgacac cagcgugggg       60

aucgugggcc uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc      120

uacuacaugu accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug      180

ggcaugaaca agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug      240

uccuacgagu gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc      360

cggucccgcc gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      480

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      540

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      600

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      660

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      780

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      840

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      900

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      960

uccaagaaga ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc     1020

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac     1080

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc     1140

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug     1200

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1380

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1440

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1500

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1560

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1620

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1680

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1740

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1800

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1860

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1920

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1980

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     2040

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     2100

gugagcgccu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3382
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3382
gggagaaagc uuaccaugaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3383
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3383
gggagaaagc uuaccaugga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3384
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3384
gggagaaagc uuaccaugaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3385
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3385
gggagaaagc uuaccaugaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3386
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3386
gggagaaagc uuaccaugcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3387
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3387
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3388
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3388
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3389
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3389
gggagaaagc uuaccauggc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  3390
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3390
gggagaaagc uuaccaugga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3391
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5616 ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 
       1 GC

<400>  3391
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3392
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3392
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgcag ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3393
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3393
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccgc agucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3394
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3394
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcggggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3395
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 GC

<400>  3395
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcgcagucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3396
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 1 GC

<400>  3396
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3397
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 1 GC

<400>  3397
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacacg      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac cacgagcacc ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacgcgcaag      420

cugcagaccc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccacgag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg     1080

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc     1380

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cccugcugau guggcucggg     2040

cugaacgcca agaacggcuc caucagccug augugccucg cgcugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3398
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 1 GC

<400>  3398
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agcggaagcg ccggggcgcc       60

gacaccagca ucgggaucau cggccugcug cucacgaccg ccauggcggc cgagaucacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      180

uucgcgacca cgcugggcgu gaacaagugc cacgugcaga ucauggaccu cgggcacaug      240

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccugc ccucccacag cacccggaag      420

cuccagacgc gcucccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      480

guggagaacu ggaucuuccg gaaccccggc uucgcccucg ucgcgguggc caucgccugg      540

cugcuggggu ccagcaccuc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc      600

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      660

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      840

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      900

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      960

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg     1080

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug     1140

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac     1200

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc     1380

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg     1440

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug     1500

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1620

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1680

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1740

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1860

auggccgugc ucggggacac cgccugggac uucggcagcg ugggcggcgu cuucaacucc     1920

cuggggaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucgggacg cugcuggugu ggcucggccu gaacaccaag     2040

aacggcucca ucagccugac cugccucgcg cugggcggcg ugaugaucuu ccuguccacc     2100

gccgucagcg ccgacguggg gugcuccgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3399
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 1 GC

<400>  3399
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc     1380

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgcggugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3400
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 1 GC

<400>  3400
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc     1080

ggcuacgaga ccgacgagaa ccgcgccaag guggagguga cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac acggcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucucc gggcaccuca agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc gucgacaugc agacccugac gccggugggg     1680

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1800

acgcaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gaccgugcgc     1860

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3401
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 1 GC

<400>  3401
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucacccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcc      600

cccgcguaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu ggguggacgu cgugcuggag cacggcgggu gcgugaccgu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accacgaccg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggcgagg cguaccucga caagcagagc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag     1080

aaccgcgcca agguggaggu cacccccaac uccccgcggg ccgaggccac ccucggcggg     1140

uucggcagcc uggggcugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgcgggcgc cgacacgggg accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg cagccaggag     1380

ggggcggucc acaccgcccu ggccggcgcc cuggaggcgg agauggacgg ggccaagggc     1440

cgccucuucu ccggccaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggg     1500

gugagcuacu cccugugcac ggccgccuuc accuucacca aggugcccgc cgagacccuc     1560

cacggcaccg ucacggugga ggugcaguac gcggggaccg acggccccug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug accccggugg gccggcugau cacggccaac     1680

cccgucauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacagcu acaucgugau cggcgugggg gacaagaaga ucacccacca cuggcaccgc     1800

uccggcagca cgaucgggaa ggccuucgag gcgaccgucc ggggcgccaa gcgcauggcc     1860

gugcugggcg acaccgccug ggacuucggg agcgugggcg gggucuucaa cucccucggc     1920

aaggggaucc accagaucuu cggcgccgcg uucaagagcc uguucggcgg gauguccugg     1980

uucagccaga uccugaucgg cacccugcuc guguggcugg ggcugaacac gaagaacggc     2040

uccaucagcc ugaccugccu ggcccucggg ggcgugauga ucuuccucuc caccgccguc     2100

agcgccgacg ugggcugcuc cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3402
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 1 GC

<400>  3402
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccgcaag      360

aagggcgagg cccggcgcag ccggcgcgcg gugacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccggug caucggggug uccaaccgcg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cggagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcggacccug      900

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc     1080

ggcuacgaga ccgacgagaa ccgggccaag guggagguga cccccaacuc cccgcgcgcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg gaccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac acggcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucucc gggcaccuca agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc gucgacaugc agacccugac gccggugggg     1680

cggcugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc gaccgugcgg     1860

ggcgccaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3403
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 1 GC

<400>  3403
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaccgu caacgacauc     1080

ggcuacgaga ccgacgagaa ccgcgccaag guggagguga cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac acggcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucucc gggcaccuca agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc gucgacaugc agacccugac gccggugggg     1680

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1800

acgcaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gaccgugcgc     1860

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3404
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 1 GC

<400>  3404
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacc     1080

gacgagaacc gcgccaaggu cgaggugacc cccaacuccc cgcgggccga ggcgacccuc     1140

gggggcuucg ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggggccgac acgggcaccc cgcacuggaa caacaaggag     1320

gcccuggucg aguucaagga cgcccacgcg aagcggcaga ccgugguggu ccucgggagc     1380

caggagggcg ccgugcacac cgcccuggcc ggggcgcugg aggccgagau ggacggcgcc     1440

aagggcaagc uguucuccgg gcaccucaag ugccgccuga agauggacaa gcuccggcug     1500

aagggcguga gcuacucccu gugcacggcc gccuucaccu ucaccaaggu ccccgcggag     1560

acccuccacg ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc cggucgggcg ccugaucacg     1680

gccaaccccg ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg     1740

cccuucggcg acagcuacau cgugaucggg gucggcgaca agaagaucac ccaccacugg     1800

caccgguccg ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc     1920

cucgggaagg gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu gaucgggacc cugcucgugu ggcugggccu gaacacgaag     2040

aacgggucca ucagccugac cugccucgcc cugggcggcg ucaugaucuu ccucuccacc     2100

gccgugagcg ccgacguggg gugcuccguc gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3405
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 1 GC

<400>  3405
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggcg gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3406
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 1 GC

<400>  3406
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacgcgagcg uggggaucgu cggccugcuc cugaccaccg ccauggccgc cgaggugacg      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgcgggcga ggccaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac gaccagcacc uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcg gucacgcucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac cuggcuggag ucccgggagu acacgaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg ccgccgccgc gaucgccugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca cgguggacau cgagcugguc accaccaccg ugagcaacau ggcggaggug      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacg      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguucg cgugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggcgg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggccccugca aggugccggc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3407
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 1 GC

<400>  3407
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg cuggcgcugg ccgccgccgc caucgcgugg      540

cuccugggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcucguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccucgacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcuggggu cccaggaggg cgccguccac accgcgcugg ccggggcccu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3408
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 1 GC

<400>  3408
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu gguaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3409
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 1 GC

<400>  3409
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgagauc      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcucgggu cccaggaggg cgccgugcac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3410
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 1 GC

<400>  3410
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcuggc ccagauggcg gucgacaugc agacccugac cccggugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3411
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 1 GC

<400>  3411
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg acccuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagacgc gcagccagac cuggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgcgaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3412
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 1 GC

<400>  3412
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccguggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3413
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 1 GC

<400>  3413
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcgcc uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcg gucacgcucc ccagccacuc cacccggaag      420

cugcagaccc gcccccagac cuggcuggag agccgggagu acacgaagca ccucauccgc      480

guggagaacu ggaucuuccg gaacccgggg uucgcccugg ccgccgccgc gaucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca cgguggacau cgagcugguc accaccaccg uguccaacau ggcggaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacg      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg cgugcagcaa gaagaugacg ggcaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacag cccccgggcc     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggggcgg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggcccgugca aggugcccgc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgcggccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca cggccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3414
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 1 GC

<400>  3414
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag caagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3415
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 1 GC

<400>  3415
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccacccg ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccggug caucggggug uccaaccgcg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cggagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg cugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcggacccug      900

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgggcgaag gucgagauca cgccgaacag cccccgcgcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg gaccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg gcucuccagc gggcaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cgccugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccggag cggguccacg aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3416
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 1 GC

<400>  3416
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggagcgcc       60

gacaccuccg ugggcaucgu cgggcugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccggggca gcgccuacua cauguaccug gaccgcaacg acgccgggga ggcgaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acggcacgug ccaccacaag      360

aagggggagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggc uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucgggu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      660

ggcggcaccu gggucgacgu ggugcuggag cacgggggcu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggggagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcuggggcaa cgggugcggc cuguucggga agggcucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc gggaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg gcaugaucgu gaacgacacc     1080

gggcacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucggcggguu cggcucccug gggcuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggcgccg acacggggac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggca gccaggaggg ggccguccac accgcgcugg ccggcgcccu ggaggccgag     1440

auggacgggg ccaagggccg ccucuccagc ggccaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggggu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccgug acggucgagg ugcaguacgc cgggaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaccc agacccugac gcccgugggc     1680

cggcugauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg ggacuccuac aucgucaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccgcag cggcagcacc aucgggaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggcgac accgccuggg acuucggguc cgugggcggg     1920

gcccucaaca gccugggcaa ggggauccac cagaucuucg gcgccgcguu caagucccug     1980

uucggcggga ugagcugguu cucccagauc cugaucggca cgcuccugau guggcugggg     2040

cucaacacca agaacggcag caucucccug augugccugg cccucggggg cgugcugauc     2100

uuccugagca ccgccgucuc cgccgacgug ggcugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3417
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 1 GC

<400>  3417
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccaacg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccggggga gcgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgcccgcc ggauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3418
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 1 GC

<400>  3418
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gagaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3419
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 1 GC

<400>  3419
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu cccugcgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcucguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccucgacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcuggggu cccaggaggg cgccguccac accgcgcugg ccggggcccu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3420
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 1 GC

<400>  3420
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccaacg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccggggga gcgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggauguuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3421
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 1 GC

<400>  3421
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggauccugcg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cuccugggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcucguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccucgacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcuggggu cccaggaggg cgccguccac accgcgcugg ccggggcccu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg ggaucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3422
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 1 GC

<400>  3422
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc agcaccaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc gacgaucucc gacaucgcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgccguc     1380

gugcucgggu cccaggaggg cgcggugcac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc gcagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgcgaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3423
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 1 GC

<400>  3423
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3424
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 1 GC

<400>  3424
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagc ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3425
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 1 GC

<400>  3425
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gagaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

aucgagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ugaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucgggguc agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcucgggu cccaggaggg cgccgugcac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3426
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 1 GC

<400>  3426
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaucg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3427
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 1 GC

<400>  3427
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ugaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccugac ccccgugggg     1680

cggcugauca cggcgaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagcuuc augugccugg cgcugggcgg cgugcucauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3428
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 1 GC

<400>  3428
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcucagcucc gggcaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cgccugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3429
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 1 GC

<400>  3429
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gaggcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3430
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 1 GC

<400>  3430
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcgccgac     1620

ggccccugca aggugccggc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3431
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 1 GC

<400>  3431
gggagaaagc uuaccaugcg caucaucaac gccggcaagg agaagaagcg gcgcggggcc       60

gacaccaacg ugggcaucgu cgggcugcuc cugaccacgg cgauggccgc cgaggugacc      120

cggcgcggca gcgccuacua cauguaccug gaccggaacg acgccgggga ggcgaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggggugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acggcacgug ccaccacaag      360

aagggggagg cccgccggag ccgccgggcc gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucauccgg      480

guggagaacu ggaucuuccg caaccccggc uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucgggu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccggug caucggcgug uccaaccgcg acuucgugga gggcaugagc      660

gggggcaccu gggucgacgu ggugcuggag cacgggggcu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cggagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg cugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcggacccug      900

guggaccgcg gguggggcaa cgggugcggc cuguucggga agggcucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccggauca ugcugagcgu ccacgggucc cagcacagcg gcaugaucgu gaacgacacc     1080

gggcacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgcgcc     1140

gaggccaccc ucggcggguu cggcucccug ggccuggacu gcgagccccg gaccgggcuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggcgccg acacggggac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug     1380

gugcucggca gccaggaggg ggccguccac accgcgcugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaaggggcg gcucuccagc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccgug acggucgagg ugcaguacgc cggcaccgac     1620

gggcccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggc     1680

cgccugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg ggacuccuac aucgucaucg gcguggggga gaagaagauc     1800

acccaccacu ggcaccggag cggcuccacg aucggcaagg ccuucgaggc caccgugcgc     1860

ggggcccggc gcauggcggu ccugggcgac accgccuggg acuucgggag cgugggcggg     1920

gcccucaaca gccugggcaa gggcauccac cagaucuucg gggccgcguu caagucccug     1980

uucggcggga ugagcugguu cucccagauc cugaucggca cccuccugau guggcugggg     2040

cucaacacga agaacggcag caucucccug augugccugg cccucggcgg ggugcugauc     2100

uuccugagca ccgccgucuc cgccgacgug ggcugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3432
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 1 GC

<400>  3432
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gagaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaagg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3433
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 1 GC

<400>  3433
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacaugguca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgucacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcgcacg ccaagcggca gaccguggug     1380

guccucgggu cccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccugac ccccgugggg     1680

cggcugauca cggcgaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cgcucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3434
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 1 GC

<400>  3434
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccaacg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccggggga gcgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacau gaccuccacc uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacgcucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac cuggcuggag agccgggagu acacgaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca cgguggacau cgagcugguc accaccaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg gugccccacg      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca cggccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3435
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 1 GC

<400>  3435
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccgccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggcgg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggccccugca aggugccggc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagacc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3436
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 1 GC

<400>  3436
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gagaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

cuggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccga ccguggacau cgagcucguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgcccaacag cccgcgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccucgacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acacgggcac cccgcacugg     1320

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcuggggu cccaggaggg cgccguccac accgcgcugg ccggggcccu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcacgg cggccuucac cuucaccaag     1560

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcg gucgacaugc agacccugac cccggugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3437
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 1 GC

<400>  3437
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucgggauc agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggauccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacguc gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3438
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 1 GC

<400>  3438
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacacg      240

ugcgacgcca ccauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cauccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacgcca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3439
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 1 GC

<400>  3439
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccagcgguc ccgccgggcc gucacccucc ccagccacuc cacccgcaag      420

cugcagaccc ggagccagac guggcuggag ucccgcgagu acaccaagca ccucauccgg      480

guggagaacu ggaucuuccg caaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccggug caucggggug agcaaccgcg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgguccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcggacccug      900

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccggauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgcgcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg gaccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg gcucagcucc gggcaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cgccugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3440
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 1 GC

<400>  3440
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcaggcgc gcagccagac cuggcuggag ucccgggagu acacgaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg ccgccgccgc gaucgccugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca cgguggacau cgagcugguc accaccaccg ugagcaacau ggcggaggug      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacg      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguucg cgugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggcgg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggccccugca aggugccggc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3441
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 1 GC

<400>  3441
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggcgaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3442
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 1 GC

<400>  3442
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga ccaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac accggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacgcucca cgggaccgug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc gucgacaugc agacgcugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacc gagggcacgg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg ggacagcuac aucgucaucg gcguggggga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcagcacc aucggcaagg cguucgaggc caccgugcgc     1860

ggggccaagc ggauggccgu ccugggcgac acggccuggg acuucgggag cgugggcggg     1920

gugcucaacu cccugggcaa gggcauccac cagaucuucg gggcggccuu caagagccug     1980

uucggcggga uguccugguu cagccagauc cugaucggca cccuccugau guggcugggg     2040

cucaacacca agaacggcuc caucagccug augugccugg cccucggcgg gguccugauc     2100

uuccugucca ccgccgugag cgcggacgug ggcugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3443
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 1 GC

<400>  3443
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggc cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucagcaacau ggcggaggug      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg cgugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugcucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggc ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gcgggggccg ccacgggcac gccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcggca gaccguggug     1380

guccuggggu cccaggaggg cgccgugcac accgcccugg ccggggcgcu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agaccgucga cgggaccgug acgguggagg gccaguacgg cgggaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcagcacg aucgggaagg ccuucgaggc gaccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcgcucaacu cccugggcaa ggggauccac cagaucaucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggcuc caucagccug augugccugg cccucggggg cgugcugauc     2100

uuccugucca ccgccgugag cgggggcgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3444
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 1 GC

<400>  3444
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga ccaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgcguucac cuucuccaag     1560

auccccgccg agacccucca cgggaccgug acggaggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggcgaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cgcucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3445
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 1 GC

<400>  3445
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga ccaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgcgaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcag cgggagcacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu ccugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug augugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3446
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 1 GC

<400>  3446
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggu ggaggugacc      120

cgccggggga acgccuacua cauguaccug gaccgcuccg acgccggcga ggccaucagc      180

uuccccacca cgauggggau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cgcggcgcuc ccggcgcgcc gugacccugc ccagccacuc cacccggaag      420

cuccagacgc gcagccagac cuggcuggag ucccgggagu acaccaagca ccugauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccucg ccgcggccgc caucgccugg      540

cugcugggca gcuccaccag ccagaagguc aucuaccucg ugaugauccu gcugaucgcc      600

ccggcguacu ccauccgcug caucggggug agcaaccggg acuucgucga gggcaugucc      660

ggcgggacgu ggguggacgu gguccucgag cacggcgggu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcuggug accacgaccg ugagcaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcuc      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccucgag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag guggagauca ccccgaacag cccccgggcc     1140

gaggcgaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccug     1200

gacuucagcg accucuacua ccugacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu cccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcccacg cgaagcggca gaccguggug     1380

guccuggggu cccaggaggg cgccgugcac accgcccucg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuca agugccggcu gaagauggac     1500

aagcugcgcc ucaagggcgu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccugca cgggacgguc accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccucac gcccgugggg     1680

cggcugauca ccgccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcucgacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg cguucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcgcugaacu cccucgggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu cuggcugggc     2040

cugaacacca agaacggguc caucagccuc augugccugg cccugggcgg cgugcucauc     2100

uuccugucca ccgcggugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3447
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 1 GC

<400>  3447
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga ccaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgcgaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcag cgggagcacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu ccugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug gugugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3448
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 1 GC

<400>  3448
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggu ggaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cgcggcgcag ccggcgcgcc gugacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg ccgcggccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcc      600

ccggcguaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggacgu ggguggacgu gguccuggag cacggcgggu gcgugaccgu gauggcccag      720

gacaagcccg ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcgcacg ccaagcggca gaccguggug     1380

guccucggga gccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccugac ccccgugggg     1680

cggcugauca cggcgaaccc cgucaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cgggagcacg aucggcaagg ccuucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccuggu guggcugggc     2040

cucaacacga agaacgggag caucucccug accugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacguc gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3449
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 1 GC

<400>  3449
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgu ggaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga cgaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc uggguggucu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgu caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcc      600

ccggcguaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggcgagg cguaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcc     1140

gaggcgaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcag cgggagcacg aucggcaagg cguucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcuc cgucgggggc     1920

gcgcucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccuggu guggcugggc     2040

cucaacacga agaacgggag caucucccug accugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgcggucuc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3450
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 1 GC

<400>  3450
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgu ggaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cccucggggu caacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggcgaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1800

acccaccacu gguaccgcag cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccuggu guggcugggc     2040

cucaacacga agaacgggag caucucccug accugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccgucuc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3451
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 1 GC

<400>  3451
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgu ggaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      180

uuccccacca cccucggggu caacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca ccccgaacag cccccgggcg     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg ccgcguucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accguggagg uccaguacgc cggcacggac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca ccgcgaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1800

acccaccacu ggcaccgcag cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccuggu guggcugggc     2040

cucaacacca agaacgggag caucucccug acgugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccgucuc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3452
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 1 GC

<400>  3452
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucacccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcc      600

cccgcguaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu ggguggacgu cgugcuggag cacggcgggu gcgugaccgu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accacgaccg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggcgagg cguaccucga caagcagagc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacuc cccgcgggcc     1140

gaggccaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg cacgggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gacggucgug     1380

gugcucggga gccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggcggag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacgcucca cgggaccguc accguggagg ugcaguacgc gggcaccgac     1620

ggccccugca agguccccgc ccagauggcc guggacaugc agacgcugac cccggugggg     1680

cggcugauca ccgccaaccc cgucaucacc gagagcacgg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc gaccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac acggccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca cggccgucag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3453
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 1 GC

<400>  3453
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca accucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac gaccuccacc ugggucgugu acgggaccug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacgcucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac cuggcuggag agccgcgagu acacgaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg ccgccguggc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccga cgguggacau cgagcugguc accaccaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggcca gcgacucccg gugccccacg      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguga cccccaacuc cccgcgggcg     1140

gaggccacgc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcgcacg ccaagcggca gaccguggug     1380

guccucggga gccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgcguucac guucaccaag     1560

guccccgccg agacccucca cgggaccgug accguggagg uccaguacgc cggcacggac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1680

cggcugauca ccgcgaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcgugcac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcucgu cuggcugggc     2040

cugaacacca agaacggguc caucagccuc acgugccugg cccugggcgg cgugaugauc     2100

uuccucucca ccgcggugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3454
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 1 GC

<400>  3454
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagguga cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acacgggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcacgg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg cguucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcucgu guggcugggc     2040

cugaacacga agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgugag cgcggacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3455
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 1 GC

<400>  3455
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca accucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac gaccuccacc ugggucgugu acgggaccug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacgcucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac cuggcuggag agccgcgagu acacgaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg ccgccguggc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccga cgguggacau cgagcugguc accaccaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggcca gcgacucccg gugccccacg      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag     1080

aaccgcgcca aggucgaggu gacccccaac uccccgcggg cggaggccac ccucggcggg     1140

uucggcagcc uggggcugga cugcgagccc cgcacgggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccug     1320

gucgaguuca aggacgcgca cgccaagcgg cagacggugg ugguccucgg cagccaggag     1380

ggggccgugc acaccgcccu ggcgggcgcc cuggaggccg agauggacgg ggccaagggc     1440

cgccucuucu ccggccaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggg     1500

gugagcuacu cccugugcac cgccgcguuc accuucacga agguccccgc cgagacccuc     1560

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacgcug accccggucg gccggcugau caccgcgaac     1680

cccgugauca ccgagagcac ggagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacagcu acaucgugau cggcgucggg gacaagaaga ucacccacca cuggcaccgc     1800

uccggcagca ccaucgggaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcc     1860

gugcugggcg acacggcgug ggacuucggg agcgucggcg ggguguucaa cucccucggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg gauguccugg     1980

uucagccaga uccugaucgg cacccugcuc guguggcugg ggcugaacac caagaacggc     2040

uccaucagcc ugaccugccu ggcccucggg ggcgucauga ucuuccucuc cacggcggug     2100

agcgccgacg ugggcugcuc cgucgacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3456
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 1 GC

<400>  3456
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac acggcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggacgguc accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc guggacaugc agacccugac gccggugggg     1680

cggcugauca ccgccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg cguucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgucag cgcggacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3457
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 1 GC

<400>  3457
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccagcacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acacgggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcacgg ccgugugcac cgccgcgaag     1560

guccccgccg agacccucca cgggaccgug accguggagg uccaguacgc cggcacggac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1680

cggcugauca ccgcgaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccuc acgugccugg cccugggcgg cgucaugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3458
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 1 GC

<400>  3458
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacgcgagca ucgggaucgu gucccugcuc cugaccaccg ucauggccgc cgagaucacg      120

cggcgcggca gcgccuacua cauguaccug gaccgguccg acgccgggaa ggcgaucagc      180

uucgugacca cccucggcgu gaacaagugc cacguccaga ucauggaccu ggggcacaug      240

ugcgacgcca ccauguccua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac gaccagcacc ugggucgugu acgggaccug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacgcucc ccagccacuc cacccgcaag      420

cugcagaccc ggagccagac cuggcuggag ucccgcgagu acacgaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggc uucgcgcugg uggccguggc caucgccugg      540

cugcucggga gcuccaccag ccagaagguc aucuaccugg ugaugauccu gcucaucgcc      600

cccgcguacu ccauccgcug caucggcgug agcaaccggg acuucgucga ggggaugucc      660

ggcggcaccu ggguggacgu gguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccga cggucgacau cgagcuggug accaccaccg ugagcaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cagcaucagc gacauggccu ccgacagccg gugccccacg      840

cagggggagg cguaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcuggggcaa cgggugcggc cuguucggga agggcagccu cgugaccugc      960

gccaaguuca cgugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca agguggaggu gacccccaac agcccgcggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcacggggc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugcccuggc acuccggcgc ggacaccgag accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagacggugg ucgugcucgg cagccaggag     1380

ggggccgugc acaccgcgcu ggccggcgcc cuggaggccg agauggacgg ggcgaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucuccuaca gccugugcac cgccgccuuc accuucacga aggugcccgc cgagacccuc     1560

cacggcaccg ugaccgucga ggugcaguac gccgggcggg acggccccug caaggugccc     1620

gcgcagaugg ccgucgacau gcagacccug acgccggugg ggcgccugau caccgccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggcgugggg gacaagaaga ucacgcacca cuggcaccgg     1800

uccggcagca ucaucgggaa ggccuucgag gccaccgugc gcggcgcgaa gcggauggcc     1860

guccuggggg acaccgccug ggacuucggc uccgugggcg ggguguucaa cagccucggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg cacccugcuc gucuggcugg ggcugaacac gaagaacggc     2040

agcaucuccc ugaccugccu ggcgcucggg ggcgugauga ucuuccucag caccgccgug     2100

uccgccgacg ucgggugcag cguggacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3459
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 1 GC

<400>  3459
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag     1080

aaccgcgcca agguggaggu cacccccaac uccccgcggg ccgaggcgac ccucggcggg     1140

uucggcagcc uggggcugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggg acgccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgucg uggugcucgg cagccaggag     1380

ggggccgucc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cgccucuucu ccggccaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc acguucacca aggugcccgc ggagacccuc     1560

cacggcaccg ucaccgugga ggugcaguac gccgggacgg acggccccug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug accccggugg gccggcugau caccgccaac     1680

cccgucauca cggagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacagcu acaucgugau cggcgugggg gacaagaaga ucacccacca cuggcaccgc     1800

uccggcagca ccaucgggaa ggcguucgag gccacggucc ggggcgccaa gcgcauggcc     1860

gugcugggcg acaccgccug ggacuucggg agcgugggcg gggucuucaa cucccucggc     1920

aaggggaucc accagaucuu cggcgcggcc uucaagagcc uguucggcgg gauguccugg     1980

uucagccaga uccugaucgg cacccugcuc guguggcugg ggcugaacac caagaacggc     2040

uccaucagcc ucacgugccu ggcccucggg ggcgugauga ucuuccuguc caccgccguc     2100

agcgcggacg ugggcugcuc cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3460
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 1 GC

<400>  3460
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gccggacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgaga cccgccggag ccgccggucc gugagccucc gcuaccacua cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaagaagca ccucaucaug      480

gucgagaacu ggaucuuccg caaccccggg uucgccaucg uguccguggc gaucaccugg      540

cugaugggca gccugaccuc ccagaagguc aucuaccucg ugaugaucgu gcugaucguc      600

cccgccuaca gcaucuccug caucggggug agcaaccggg accuggugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcucgag cacggcgggu gcgucacgga gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaugaccg ucagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc cagccugagc gacauggcgu ccgccagccg gugcccgacg      840

cagggcgagc ccucccucga caagcagagc gacacccagu ccgugugcaa gcgcacccug      900

ggcgaccggg gguggggcaa cgggugcggc aucuucggga agggcagccu ggugaccugc      960

uccaaguuca cgugcugcaa gaagaugccc ggcaagagca uccagcccga gaaccucgag     1020

uaccgcauca ugcugccggu ccacgggucc cagcacagcg gcaugaucgu caacgacauc     1080

gggcacgaga ccgacgagaa ccgcgccaag guggagguga cccccaacag cccccgggcc     1140

gaggccaccc ugggcggguu cggcucccuc ggccuggacu gcgagccccg cacggggcug     1200

gacuucuccg accucuacua ccugaccaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu ccccuggcac gcgggcgccg acaccgggac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcugggca gccaggaggg ggcggugcac accgcccucg ccggcgcccu ggaggccgag     1440

auggacggcg cgaaggggcg ccuguucucc ggccaccuca agugccggcu gaagauggac     1500

aagcugcgcc ucaagggggu cagcuacucc cugugcaccg ccgccuucac cuucacgaag     1560

gugcccgccg agacccugca cggcaccgug accgucgagg ugcagagcgc gggcaccgac     1620

gggccgugca aggugcccgc ccagauggcc gucgacaugc agacgcucac ccccgugggc     1680

cggcugauca ccgccaaccc cgugaucacc gaguccacgg agaacagcaa gaugaugcug     1740

gagcucgacc cgcccuucgg ggacagcuac aucgucaucg gcguggggga caagaagauc     1800

acccaccacu ggcaccgcuc cggcagcacc aucggcaagg ccuucgaggc gaccgugcgc     1860

ggggccaagc ggauggccgu ccugggcgac acggccuggg acuucggguc cgugggcggg     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gggccgcguu caagucccuc     1980

uucggcggga ugagcugguu cucccagauc cugaucggca cccugcuggu cuggcucggg     2040

cugaacacca agaacggcag caucucccug accugccucg cccugggcgg ggugaugauc     2100

uuccugagca cggccguguc cgccgacguc ggcugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3461
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 1 GC

<400>  3461
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca ccaugggggu gaacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gccggacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccccag ccggcgcgcg gugacccugc ccucccacag cacccggaag      420

cuccagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccugaucaag      480

gucgagaacu ggauccuccg caaccccggg uucaccgugg ccgcccuggc cauggccugg      540

cugcucggca acuccaccag ccagcgggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcga gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguuca cgugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguga ccccgaacag cccccgggcc     1140

gaggcgaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcccacg cgaagcggca gaccguggug     1380

guccucgggu cccaggaggg cgccgugcac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucagc gggcaccuga agugccggau caagauggac     1500

aagcugcgcc ucaagggcgu guccuacagc cugugcaccg ccgccuucac cuucuccaag     1560

guccccgcgg agacccugca cgggaccgug acgguggagg uccaguacgc cggccgggac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccucac ccccgucggg     1680

cgccugauca ccgccaaccc caucaucacg gagagcaccg agaacucccg gaugaugcug     1740

gagcucgacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg cguucgaggc cacgguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccuggggaa gggcgugcac cagguguucg gcgcggccuu caagagccuc     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcuggu cuggcucggc     2040

cugaacacca agaacggguc caucagccug gccugccucg cccugggcgg cgcgaugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg ucgacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  3462
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 1 GC

<400>  3462
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3463
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 1 GC

<400>  3463
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3464
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 1 GC

<400>  3464
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccgaga ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguu cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcga agcggauggc cgugcucggg gacaccgccu gggacuucgg cagcgugggc     1860

ggcgucuuca acucccuggg gaaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg ggacgcugcu gguguggcuc     1980

ggccugaaca ccaagaacgg cuccaucagc cugaccugcc ucgcgcuggg cggcgugaug     2040

aucuuccugu ccaccgccgu cagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3465
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 1 GC

<400>  3465
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3466
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 1 GC

<400>  3466
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcgugcacg gcucccagca cagcggcaug     1020

aucgucaacg acaccgggua cgagaccgac gagaaccgcg ccaaggugga ggugaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacc     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggucgugcu cggcagccag gagggggccg ugcacacggc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggcaagcugu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaagauc cccgugcaga uggcggucga caugcagacc     1620

cugacgccgg ugggccggcu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggacaaga agaucacgca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg ugcggggcgc caagcgcaug gcgguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcug     1980

cucgucuggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccucgcccuc     2040

gggggcguga ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3467
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 1 GC

<400>  3467
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc ggaagaaggg ggaggcccgc cggagccgcc gggccgugac ccuccccucc      360

cacagcaccc gcaagcugca gacccggucc cagacguggc uggagagccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccggaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcgugcacg gcucccagca cagcggcaug     1020

aucgucaacg acaccgggua cgagaccgac gagaaccggg ccaaggugga ggugaccccc     1080

aacuccccgc gcgccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacc     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggccg ugcacacggc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggcaagcugu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaagauc cccgugcaga uggcggucga caugcagacc     1620

cugacgccgg ugggccgccu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggacaaga agaucacgca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gcgguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcug     1980

cucgucuggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccucgcccuc     2040

gggggcguga ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3468
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 1 GC

<400>  3468
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcgugcacg gcucccagca cagcggcaug     1020

accgucaacg acaucgggua cgagaccgac gagaaccgcg ccaaggugga ggugaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacc     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggucgugcu cggcagccag gagggggccg ugcacacggc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggcaagcugu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaagauc cccgugcaga uggcggucga caugcagacc     1620

cugacgccgg ugggccggcu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggacaaga agaucacgca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg ugcggggcgc caagcgcaug gcgguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcug     1980

cucgucuggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccucgcccuc     2040

gggggcguga ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3469
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 1 GC

<400>  3469
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucac ccugguggcc      480

gucgcgaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gccgaccgug gacaucgagc uggugaccac gaccgucucc      720

aacauggcgg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cgcuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acgagaaccg cgcgaaggug gaggucaccc ccaacucccc gcgggccgag     1080

gccacccucg ggggcuucgg gagccugggc cuggacugcg agccccgcac cgggcucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca cgggcacccc gcacuggaac     1260

aacaaggagg cgcuggugga guucaaggac gcccacgcca agcggcagac cgucguggug     1320

cucgggagcc aggagggcgc cguccacacc gcccuggcgg gggcccugga ggccgagaug     1380

gacggcgcca aggggcgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggcgugag cuacucccug ugcacggcgg ccuucaccuu caccaaggug     1500

cccgccgaga cccuccacgg gaccgucacg guggaggugc aguacgccgg caccgacggg     1560

cccugcaagg uccccgccca gauggcggug gacaugcaga cccugacccc ggugggccgc     1620

cugaucacgg ccaaccccgu caucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucggcga cagcuacauc gugaucgggg ugggcgacaa gaagaucacc     1740

caccacuggc accgguccgg gagcacgauc ggcaaggccu ucgaggccac cguccgcggg     1800

gccaagcgga uggcggugcu gggcgacacc gccugggacu ucggcagcgu cgggggcgug     1860

uucaacuccc ucgggaaggg cauccaccag aucuucgggg ccgccuucaa gagccuguuc     1920

ggcggcaugu ccugguucag ccagauccug aucgggaccc ugcucgugug gcugggccug     1980

aacacgaaga acggguccau cagccugacc ugccucgccc ugggcggggu gaugaucuuc     2040

cucuccaccg cggucagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3470
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 1 GC

<400>  3470
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcgugcacg gcucccagca cagcggcaug     1020

aucggguacg agaccgacga gaaccgcgcc aaggucgagg ugacccccaa cuccccgcgg     1080

gccgaggcca cccucggcgg guucggcagc cuggggcugg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg cggacacggg gaccccgcac     1260

uggaacaaca aggaggcccu ggucgaguuc aaggacgccc acgccaagcg gcagaccgug     1320

gugguccucg gcagccagga gggggccgug cacaccgcgc uggccggcgc ccuggaggcc     1380

gagauggacg gggcgaaggg caagcuguuc uccggccacc ugaagugccg ccugaagaug     1440

gacaagcucc gccugaaggg ggugagcuac ucccugugca cggccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gugacggugg agguccagua cgccgggacc     1560

gacggccccu gcaagauccc cgugcagaug gcgguggaca ugcagacccu gaccccgguc     1620

ggccggcuga ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug     1680

cucgagcugg acccgcccuu cggggacagc uacaucguga ucggcgucgg ggacaagaag     1740

aucacccacc acuggcaccg cuccggcagc acgaucggga aggccuucga ggccaccgug     1800

cggggcgcca agcgcauggc ggugcugggc gacaccgccu gggacuucgg gagcgucggc     1860

gggguguuca acucccucgg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg ggauguccug guucagccag auccugaucg gcacccugcu cguguggcug     1980

gggcugaaca cgaagaacgg cuccaucagc cugaccugcc ucgcccucgg gggcgucaug     2040

aucuuccucu ccaccgcggu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3471
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 1 GC

<400>  3471
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggccggggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3472
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 1 GC

<400>  3472
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggccuggc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggucaug      540

auccuccuga ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc ucgucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccucuu cgggaagggc      900

agccugguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcuc uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cucguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu gggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcucgagg ccgagaugga cggggccaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cugcacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3473
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 1 GC

<400>  3473
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacugguac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3474
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 1 GC

<400>  3474
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga cacccagacc     1620

cugacgcccg ugggccggcu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cgcuccggca gcaccaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3475
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 1 GC

<400>  3475
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg agauccgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg uggucgugcu cggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3476
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 1 GC

<400>  3476
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug cuggcccaga uggccgucga caugcagacc     1620

cugaccccgg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3477
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 1 GC

<400>  3477
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgacccu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcacgugggu gguguacggg      300

accugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacgcgcagc cagaccuggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg cacguggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga ccugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg gcagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagacggac gagaaccggg ccaaggucga gaucaccccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacgcccg ugggccggcu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cgcuccggca gcaccaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3478
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 1 GC

<400>  3478
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccguc gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3479
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 1 GC

<400>  3479
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcgcgugggu gguguacggg      300

accugccacc acaagaaggg cgaggcccgg cgcucccggc gcgccgucac gcuccccagc      360

cacuccaccc ggaagcugca gacccgcccc cagaccuggc uggagagccg ggaguacacg      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc cgggcuucgc ccuggcggcc      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgcgcccgc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gcccacggug gacaucgagc uggucaccac caccgugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggcguccgac      780

agccggugcc ccacgcaggg ggaggccuac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucgccugc agcaagaaga ugacggggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucaccccc     1080

aacagccccc gcgcggaggc cacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcg     1500

uucacguuca ccaagauccc cgccgagacc cuccacggca ccgugaccgu cgaggugcag     1560

uacgccggga cggacggccc gugcaaggug cccgcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcg aaccccguga ucacggaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggcu ccaccaucgg gaaggccuuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc gugggacuuc     1860

gggagcgugg gcggggcccu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcagcaucu cccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu gagcacggcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3480
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 1 GC

<400>  3480
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagcaaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3481
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 1 GC

<400>  3481
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc acccgccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccgcugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccgcg ccaaggucga gaucacgccg     1080

aacagccccc gggccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcucu ccagcggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccgccu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccacgaucgg gaaggcguuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcagcaucu cccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3482
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 1 GC

<400>  3482
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcacccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacgcccg ugggccggcu gaucaccgcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cgcagcggcu ccaccaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacgcuc     1980

cugauguggc uggggcucaa caccaagaac ggcagcaucu cccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3483
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 1 GC

<400>  3483
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacucccug cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc ucgucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccucuu cgggaagggc      900

agccugguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcuc uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cucguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu gggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcucgagg ccgagaugga cggggccaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cugcacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3484
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 1 GC

<400>  3484
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcaucga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggugaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac cacggucagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg uggucgugcu cggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3485
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 1 GC

<400>  3485
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc cugcggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggucaug      540

auccuccuga ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc ucgucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccucuu cgggaagggc      900

agccugguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcuc uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cucguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu gggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcucgagg ccgagaugga cggggccaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cugcacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcauc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcucg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3486
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 1 GC

<400>  3486
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucagcac caccgugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccacga ucuccgacau cgccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ccgucgugcu cggcucccag gagggggccg ugcacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcg     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcg aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggccuuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc gugggacuuc     1860

gggagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3487
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 1 GC

<400>  3487
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgagcugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcucg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3488
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 1 GC

<400>  3488
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caucgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggcguuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccacggcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3489
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 1 GC

<400>  3489
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggugaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucacggu ggaggugcag     1560

uacgcgggga ccgacggccc cugcaagguc ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggccuuc     1800

gaggcgaccg uccggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gcuucaugug ccuggcccuc     2040

gggggcgucc ugaucuuccu guccaccgcc gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3490
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 1 GC

<400>  3490
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggcaagcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccgccu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgguccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3491
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 1 GC

<400>  3491
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caaggacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3492
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 1 GC

<400>  3492
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggaggc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3493
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 1 GC

<400>  3493
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc ggcccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcg     1500

uucacguuca ccaagauccc cgccgagacc cuccacggca ccgugaccgu cgaggugcag     1560

uacgccggga cggacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcg aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc gugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agacccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3494
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 1 GC

<400>  3494
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccgggg ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcg aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc gugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccacggcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3495
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 1 GC

<400>  3495
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgcccuggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gccgaccgug gacaucgagc ucgucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccucuu cgggaagggc      900

agccugguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcuc uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccc     1080

aacagcccgc gcgccgaggc gacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacg     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu gggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcucgagg ccgagaugga cggggccaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug cacggccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cugcacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug cccgcccaga uggccgucga caugcagacc     1620

cugaccccgg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3496
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 1 GC

<400>  3496
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggucagcgac      780

agccggugcc ccacccaggg ggaggccuac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguca cgugcgcgaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc cacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgcggg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cuggucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggugguccu cggcucccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucacggu ggaggugcag     1560

uacgcgggga ccgacggccc cugcaagguc ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggccuuc     1800

gaggcgaccg uccggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgucc ugaucuuccu guccaccgcc gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3497
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 1 GC

<400>  3497
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcaucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac cacggucagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggca uccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucuccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggcu ccacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcagcaucu cccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3498
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 1 GC

<400>  3498
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgccaccaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcacgugggu gguguacggg      300

accugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaucc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg gcagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cgccaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccacggcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3499
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 1 GC

<400>  3499
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcag cggucccgcc gggccgucac ccuccccagc      360

cacuccaccc gcaagcugca gacccggagc cagacguggc uggagucccg cgaguacacc      420

aagcaccuca uccgggugga gaacuggauc uuccgcaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cggugcaucg gcgugagcaa ccgcgacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgguc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccgcugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg gaucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccgcg ccaaggucga gaucacgccg     1080

aacagccccc gggccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cggcagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccgccu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgguccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3500
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 1 GC

<400>  3500
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca ggcccgcagc cagaccuggc uggagucccg ggaguacacg      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggcggcc      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgcgccggc cuacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gcccacggug gacaucgagc uggucaccac caccgugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggcgagcgac      780

agccggugcc ccacgcaggg ggaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga ccugcgccaa guucgccugc uccaagaaga ugacgggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucaccccg     1080

aacagccccc gcgcggaggc cacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcg     1500

uucacguuca ccaagauccc cgccgagacc cuccacggca ccgugaccgu cgaggugcag     1560

uacgccggga cggacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcg aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc gugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccacggcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3501
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 1 GC

<400>  3501
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuucu ccaagauccc cgcggagacc cuccacggca ccgugaccga ggagguccag     1560

uacgccggga cggacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucaccgcc aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggcu ccaccaucgg gaaggcguuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccacggcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3502
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 1 GC

<400>  3502
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacacggg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggcgg uccacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgcgggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacgcccg ugggccggcu gaucaccgcc aaccccguga ucaccgaggg gaccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggcgaca gcuacaucgu caucggggug     1740

ggcgagaaga agaucacgca ccacuggcac cgcuccggga gcaccaucgg caaggccuuc     1800

gaggcgaccg ugcggggcgc caagcgcaug gccguccugg gggacaccgc cugggacuuc     1860

ggcagcgucg ggggcgugcu caacucccug gggaagggca uccaccagau cuucggcgcc     1920

gcguucaaga gccuguucgg gggcaugucc ugguucagcc agauccugau cgggacgcuc     1980

cugauguggc ugggccucaa caccaagaac ggguccauca gccugaugug ccuggcccuc     2040

ggcggcgugc ugaucuuccu guccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3503
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 1 GC

<400>  3503
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

ggguccgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcagcaucu cccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3504
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 1 GC

<400>  3504
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcaacgcg uacuacaugu accuggaccg cuccgacgcc      120

ggggaggcca ucagcuuccc caccaccaug ggcaugaaca agugcuacau ccagaucaug      180

gaccucgggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu ggucuacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcucccggc gcgcggugac ccugcccagc      360

cacuccaccc ggaagcucca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuga uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccucgccgcc      480

gcggccaucg ccuggcugcu ggggagcucc accagccaga aggucaucua ccucgugaug      540

auccugcuga ucgccccggc cuacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

gucgagggga uguccggcgg caccugggug gacguggucc ucgagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gcccaccguc gacaucgagc uggugaccac cacggugagc      720

aacauggccg agguccgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggccuac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccucgugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccugguca cgugcgcgaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc ucgaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc cacccugggc ggguucggcu cccucgggcu ggacugcgag     1140

ccccggaccg gccuggacuu cagcgaccuc uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccucccgu ggcacgcggg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cuggucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggcgg ugcacaccgc ccucgccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccuga gcuccggcca ccucaagugc     1440

cggcugaaga uggacaagcu gcgccucaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgucacggu ggaggugcag     1560

uacgcgggga ccgacggccc cugcaagguc ccggcccaga uggccgugga caugcagacc     1620

cucacccccg ugggccggcu gaucacggcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu cgacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggccuuc     1800

gaggcgaccg uccggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcccu gaacucccuc ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucgucuggc uggggcugaa cacgaagaac ggcuccauca gccucaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcc gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3505
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 1 GC

<400>  3505
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

ggguccgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcagcaucu cccuggugug ccuggcccuc     2040

gggggcgucc ugaucuuccu gagcaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3506
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 1 GC

<400>  3506
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcgugaacaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccuggugug ccuggcccuc     2040

gggggcgucc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3507
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 1 GC

<400>  3507
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcuccgcg uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu ggucuacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgcggugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcc      480

gcggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gcccgccguc gacaucgagc uggugaccac caccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacgcaggg ggaggcguac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucgccugc agcaagaaga ugacggggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucaccccg     1080

aacagccccc gcgccgaggc gacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cuggucgagu ucaaggacgc ccacgcgaag     1320

cgccagaccg uggugguccu cggcagccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucacguuca ccaagauccc cgcggagacc cuccacggca ccgucaccgu ggaggugcag     1560

uacgccggga cggacggccc cugcaagguc ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcc aaccccguca ucacggaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggca gcaccaucgg gaaggcguuc     1800

gaggccacgg uccggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggguccgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cuggucuggc uggggcucaa caccaagaac ggcagcaucu cccugacgug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3508
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 1 GC

<400>  3508
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcuccgcg uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu ggucuacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgcggugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcc      480

gcggucaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgccccggc cuacagcauc cgcugcaucg gcgucuccaa ccgggacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggcgg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgcgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc cacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgcggacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggccg ugcacaccgc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucuccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcgcaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggca gcacgaucgg gaaggccuuc     1800

gaggccaccg ugcggggcgc gaagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggguccgucg gcggggcccu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gcguucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugguguggc uggggcucaa cacgaagaac ggcagcaucu cccugaccug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3509
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 1 GC

<400>  3509
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcuccgcg uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcgucaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgcggugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcc      480

gcggccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgccccggc cuacagcauc cgcugcaucg gcgucuccaa ccgggacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gcccaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc cacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgcggg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucuccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgugacggu ggagguccag     1560

uacgcgggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcguc     1740

ggggacaaga agaucaccca ccacugguac cgcagcggca gcacgaucgg gaaggccuuc     1800

gaggcgaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggguccgucg gcggggcccu caacagccug ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugguguggc uggggcucaa cacgaagaac ggcagcaucu cccugaccug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcc gucuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3510
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 1 GC

<400>  3510
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcuccgcg uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuuccc caccacccuc ggcgucaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgcggugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcc      480

gcggccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgccccggc cuacagcauc cgcugcaucg gcgucuccaa ccgggacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gcccaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaucgggca cgagaccgac gagaaccggg ccaaggugga gaucaccccg     1080

aacagccccc gcgccgaggc cacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgcggg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucuccu acagccugug caccgccgcc     1500

uucacguuca ccaagauccc cgccgagacc cuccacggca ccgugaccgu ggagguccag     1560

uacgcgggga cggacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucaccgcc aaccccguga ucacggaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcguc     1740

ggggacaaga agaucaccca ccacuggcac cgcagcggca gcaccaucgg gaaggccuuc     1800

gaggcgacgg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggguccgucg gcggggcccu caacagccug ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugguguggc uggggcucaa caccaagaac ggcagcaucu cccugacgug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcc gucuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3511
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 1 GC

<400>  3511
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucac ccugguggcc      480

gucgcgaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gccgaccgug gacaucgagc uggugaccac gaccgucucc      720

aacauggcgg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cgcuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccgcg cgaaggugga ggucaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcacgg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcg cugguggagu ucaaggacgc ccacgccaag     1320

cggcagacgg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgcggcc     1500

uucaccuuca ccaaggugcc cgccgagacg cuccacggca ccgucaccgu ggaggugcag     1560

uacgccggga ccgacggccc cugcaagguc cccgcccaga uggcggugga caugcagacg     1620

cugaccccgg ugggccgccu gaucaccgcc aaccccguca ucaccgagag cacggagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggacaaga agaucaccca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gcggugcugg gcgacacggc cugggacuuc     1860

gggagcgugg gcggggucuu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucguguggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccuggcccuc     2040

gggggcguga ugaucuuccu cuccacggcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3512
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 1 GC

<400>  3512
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccaaccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacacgaccu ccaccugggu cguguacggg      300

accugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac gcuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagaccuggc uggagagccg ggaguacacg      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccuggcggcc      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgcccccgc guacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gccgacggug gacaucgagc uggucaccac caccgugucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacgcaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaucgggca cgagaccgac gagaaccgcg ccaaggucga ggugaccccc     1080

aacuccccgc gggccgaggc cacgcucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugcccu ggcacgcggg cgccgacacc     1260

gggacgccgc acuggaacaa caaggaggcc cuggucgagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggugguccu cggcagccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucacguuca ccaagguccc cgccgagacc cuccacggca ccgugaccgu ggagguccag     1560

uacgcgggga cggacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugaccccgg ucggccgccu gaucaccgcc aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggacaaga agaucaccca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggcgacgg ugcgcggcgc caagcggaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcgggguguu caacucccuc ggcaaggggg ugcaccagau cuucggcgcc     1920

gcguucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucgucuggc uggggcugaa caccaagaac ggcuccauca gccucacgug ccuggcccug     2040

ggcggcguga ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3513
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 1 GC

<400>  3513
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcgugcacg gcucccagca cagcggcaug     1020

aucgucaacg acaucgggca cgagaccgac gagaaccgcg ccaaggugga ggugaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacg     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggucgugcu cggcagccag gagggggccg ugcacaccgc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug cacggccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug cccgcgcaga uggccgucga caugcagacc     1620

cugaccccgg ugggccgccu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggacaaga agaucaccca ccacuggcac cgguccggca gcacgaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc gaagcggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucgucuggc uggggcugaa cacgaagaac ggcuccauca gccugaccug ccuggcgcuc     2040

gggggcguga ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3514
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 1 GC

<400>  3514
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccaaccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacacgaccu ccaccugggu cguguacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac gcuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagaccuggc uggagagccg ggaguacacg      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccuggcggcc      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgcccccgc guacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gccgacggug gacaucgagc uggucaccac caccgugucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacgcaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acgagaaccg cgccaagguc gaggugaccc ccaacucccc gcgggccgag     1080

gccacccucg ggggcuucgg gagccugggc cuggacugcg agccccgcac ggggcucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcg ggcgccgaca ccggcacccc gcacuggaac     1260

aacaaggagg cccuggucga guucaaggac gcccacgcca agcggcagac gguggugguc     1320

cucgggagcc aggagggcgc ggugcacacc gcccuggccg gggcccugga ggcggagaug     1380

gacggcgcca aggggcgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggcgugag cuacucccug ugcaccgccg ccuucaccuu cacgaagguc     1500

cccgccgaga cccuccacgg gaccgugacc guggaggucc aguacgcggg caccgacggg     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cgcugacccc ggucggccgc     1620

cugaucaccg ccaaccccgu gaucaccgag agcacggaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucggcga cagcuacauc gugaucgggg ucggcgacaa gaagaucacc     1740

caccacuggc accgguccgg gagcaccauc ggcaaggccu ucgaggcgac cgugcgcggg     1800

gccaagcgga uggccgugcu gggcgacacg gccugggacu ucggcagcgu cgggggcgug     1860

uucaacuccc ucgggaaggg cauccaccag aucuucgggg ccgcguucaa gagccuguuc     1920

ggcggcaugu ccugguucag ccagauccug aucgggaccc ugcucgugug gcugggccug     1980

aacaccaaga acggguccau cagccugacc ugccucgccc ugggcggggu caugaucuuc     2040

cucuccacgg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3515
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 1 GC

<400>  3515
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

gucgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gccgaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccgcg ccaaggugga ggucaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacc     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg ucguggugcu cggcagccag gagggggccg uccacacggc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc cugcaagguc cccgcgcaga uggccgugga caugcagacc     1620

cugacgccgg ugggccgccu gaucaccgcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggacaaga agaucacgca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc gaagcggaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggucuu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcug     1980

cucguguggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccuggcgcuc     2040

gggggcguga ugaucuuccu cuccaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3516
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 1 GC

<400>  3516
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccagc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

gucgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gccgaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaucgggca cgagaccgac gagaaccgcg ccaaggugga ggucaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacg     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug cacggccgug     1500

ugcaccgccg ccaagguccc cgccgagacc cuccacggca ccgugaccgu ggagguccag     1560

uacgcgggga cggacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugaccccgg ucggccgccu gaucaccgcc aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggacaaga agaucaccca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggcgacgg ugcgcggcgc caagcggaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gcguucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucguguggc uggggcugaa caccaagaac ggcuccauca gccucacgug ccuggcccuc     2040

gggggcguca ugaucuuccu guccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3517
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 1 GC

<400>  3517
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

gucgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gccgaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acgagaaccg cgccaaggug gaggucaccc ccaacucccc gcgggccgag     1080

gccacccucg ggggcuucgg gagccugggc cuggacugcg agccccgcac cgggcucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgcggaca ccggcacgcc gcacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcggcagac cgucguggug     1320

cucgggagcc aggagggcgc cguccacacc gcgcuggccg gggcccugga ggccgagaug     1380

gacggcgcga aggggcgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggcgugag cuacucccug ugcaccgccg ccuucacguu caccaaggug     1500

cccgccgaga cccuccacgg gaccgucacc guggaggugc aguacgccgg cacggacggg     1560

cccugcaagg uccccgcgca gauggccgug gacaugcaga cccugacccc ggugggccgc     1620

cugaucaccg ccaaccccgu caucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucggcga cagcuacauc gugaucgggg ugggcgacaa gaagaucacc     1740

caccacuggc accgguccgg gagcaccauc ggcaaggccu ucgaggccac gguccgcggg     1800

gcgaagcgga uggccgugcu gggcgacacc gccugggacu ucggcagcgu cgggggcgug     1860

uucaacuccc ucgggaaggg cauccaccag aucuucgggg ccgccuucaa gagccuguuc     1920

ggcggcaugu ccugguucag ccagauccug aucgggaccc ugcucgugug gcugggccug     1980

aacaccaaga acggguccau cagccucacg ugccuggcgc ugggcggggu gaugaucuuc     2040

cucuccaccg ccgucagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3518
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 1 GC

<400>  3518
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagccgg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggagacccgg cgcagccggc gcuccgugag ccuccgguac      360

cacuacaccc gcaagcugca gacccggucc cagacguggc uggagagccg cgaguacaag      420

aagcaccuca ucauggucga gaacuggauc uuccggaacc ccggcuucgc caucgugucc      480

guggccauca ccuggcugau ggggagccug accucccaga aggucaucua ccucgugaug      540

aucgugcuga ucguccccgc cuacagcauc uccugcaucg gcgugagcaa ccgcgaccug      600

guggagggga uguccggcgg caccuggguc gacguggugc ucgagcacgg gggcugcguc      660

acggagaugg cgcaggacaa gcccaccgug gacaucgagc uggugaccau gaccgucagc      720

aacauggccg aggugcgguc cuacugcuac gaggccagcc ugagcgacau ggccuccgcc      780

agccgcugcc cgacgcaggg ggagcccucc cucgacaagc agagcgacac ccaguccgug      840

ugcaagcgga cccugggcga ccgcggcugg gggaacggcu gcgggaucuu cggcaagggg      900

agccugguga ccugcuccaa guucacgugc ugcaagaaga ugcccggcaa gagcauccag      960

cccgagaacc ucgaguaccg gaucaugcug ccggugcacg gcucccagca cagcgggaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg cgaaggugga ggugaccccc     1080

aacagccccc gggccgaggc cacccucggg ggcuucgggu cccugggccu ggacugcgag     1140

ccccgcacgg gccuggacuu cuccgaccuc uacuaccuga ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcuccccu ggcacgccgg ggccgacacc     1260

ggcacgcccc acuggaacaa caaggaggcg cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggucgugcu ggggagccag gagggcgccg ugcacaccgc ccucgcgggg     1380

gcccuggagg ccgagaugga cggcgccaag ggccgccugu ucuccgggca ccucaagugc     1440

cgccugaaga uggacaagcu gcggcucaag ggcgucagcu acucccugug caccgccgcg     1500

uucaccuuca cgaaggugcc cgccgagacc cugcacggga ccgugaccgu cgaggugcag     1560

agcgccggca ccgacggccc gugcaaggug cccgcccaga uggcggucga caugcagacg     1620

cucacccccg uggggcgccu gaucaccgcc aaccccguga ucaccgaguc cacggagaac     1680

agcaagauga ugcuggagcu cgacccgccc uucggcgaca gcuacaucgu caucggggug     1740

ggcgacaaga agaucaccca ccacuggcac cgguccggga gcaccaucgg caaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gcgguccugg gggacacggc cugggacuuc     1860

ggcuccgucg ggggcguguu caacagccug gggaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg gggcaugagc ugguucuccc agauccugau cgggacccug     1980

cuggucuggc ucggccugaa caccaagaac gggagcaucu cccugaccug ccucgcccug     2040

ggcggcguga ugaucuuccu gagcacggcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3519
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 1 GC

<400>  3519
gggagaaagc uuaccaugag cgugggcauc gucgggcugc uccugaccac cgccauggcc       60

gcggagguga cgcgccgggg cuccgccuac uacauguacc uggaccgcaa cgacgccggg      120

gaggccauca gcuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac      180

cuggggcaca ugugcgacgc caccaugucc uacgagugcc cgaugcugga cgagggcgug      240

gagcccgacg acgucgacug cuggugcaac acgaccagca ccuggguggu guacgggacc      300

ugccaccaca agaaggggga ggcgcggcgc ucccggcgcg ccgucacgcu ccccagccac      360

uccacccgga agcugcagac ccgcagccag accuggcugg agucccggga guacacgaag      420

caccucaucc gcguggagaa cuggaucuuc cggaaccccg gcuucgcccu ggccgcggcc      480

gccaucgccu ggcugcucgg gagcuccacc agccagaagg ugaucuaccu ggucaugauc      540

cugcucaucg ccccggcgua cuccauccgc ugcaucggcg ugagcaaccg ggacuucgug      600

gaggggaugu ccggcggcac cugggucgac guggugcugg agcacggggg cugcgucacc      660

gugauggccc aggacaagcc cacgguggac aucgagcugg ucaccaccac cgugagcaac      720

auggccgagg ugcgcuccua cugcuacgag gccagcaucu ccgacauggc cagcgacagc      780

cggugcccca cgcaggggga ggcguaccuc gacaagcagu ccgacaccca guacgucugc      840

aagcgcaccc ugguggaccg gggcuggggc aacgggugcg gccuguucgg gaagggcagc      900

cucgugaccu gcgccaaguu cgccugcucc aagaagauga cgggcaagag cauccagccc      960

gagaaccugg aguaccgcau caugcugucc guccacggca gccagcacuc cggcaugauc     1020

gugaacgaca ccgggcacga gaccgacgag aaccgggcca aggucgagau caccccgaac     1080

agcccccgcg ccgaggcgac gcucggcggg uucggcuccc uggggcugga cugcgagccc     1140

cggaccggcc ucgacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcuc     1200

gugcacaagg agugguucca cgacaucccc cugccguggc acgccggcgc cgacaccggg     1260

acgccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgcgaagcgc     1320

cagaccgucg uggugcucgg cucccaggag ggggccgucc acaccgcccu ggccggcgcg     1380

cuggaggccg agauggacgg ggccaagggc cgccucagcu ccggccaccu gaagugccgg     1440

cugaagaugg acaagcuccg ccugaagggg gugagcuacu cccugugcac cgccgccuuc     1500

acguucacca agauccccgc ggagacccuc cacggcaccg ugaccgucga ggugcaguac     1560

gccgggacgg acggccccug caaggugccg gcccagaugg ccgucgacau gcagacccug     1620

acccccgugg gccggcugau caccgccaac cccgugauca cggagagcac cgagaacucc     1680

aagaugaugc ucgagcugga cccgcccuuc ggggacagcu acaucgucau cggcgugggg     1740

gagaagaaga ucacccacca cuggcaccgc uccggcagca ccaucgggaa ggcguucgag     1800

gccacggugc ggggcgccaa gcgcauggcc guccugggcg acaccgccug ggacuucggg     1860

agcgugggcg gggcgcucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggcgg gauguccugg uucagccaga uccugaucgg cacccuccug     1980

auguggcugg ggcucaacac caagaacggc uccaucagcc ugaugugccu ggcccucggg     2040

ggcgugcuga ucuuccuguc cacggcgguc agcgccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  3520
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 1 GC

<400>  3520
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc ccgccggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3521
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 1 GC

<400>  3521
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggaug uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3522
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 1 GC

<400>  3522
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaugacca gcaccugggu gguguacggg      300

accugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac gcuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagaccuggc uggagucccg ggaguacacg      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gcccacggug gacaucgagc uggucaccac caccgugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacgcaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga ccugcgccaa guucgccugc uccaagaaga ugacgggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg gcagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucaccccg     1080

aacagccccc gcgccgaggc gacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucacguuca ccaagauccc cgcggagacc cuccacggca ccgugaccgu cgaggugcag     1560

uacgccggga cggacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcc aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggcguuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccacggcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3523
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 1 GC

<400>  3523
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggccaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac cacggucagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggcgagcgac      780

agccggugcc ccacccaggg ggaggccuac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

cucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggcccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgcgacg     1260

gggacgcccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccucgagg ccgagaugga cggggcgaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc gucgacggca ccgugacggu ggaggggcag     1560

uacggcggca ccgacgggcc cugcaagguc ccggcccaga uggcggugga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg caaggccuuc     1800

gaggccaccg uccgcggggc caagcggaug gcggugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggca uccaccagau caucggggcc     1920

gcguucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

ggcggggucc ugaucuuccu guccaccgcc gugagcggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3524
<211>  2275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 1 GC

<400>  3524
gggagaaagc uuaccauggg cgccgacgcc agcaucggga ucgugucccu gcuccugacc       60

accgucaugg cggccgagau cacgcgccgg ggcagcgccu acuacaugua ccuggaccgc      120

uccgacgccg ggaaggccau cagcuucgug accacccucg gcgugaacaa gugccacguc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acacgaccag caccuggguc      300

guguacggga ccugccacca caagaagggg gaggcccggc gcucccggcg cgccgugacg      360

cuccccagcc acuccacccg gaagcugcag acccgcagcc agaccuggcu ggagucccgg      420

gaguacacga agcaccucau caaggucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cugguggcgg uggccaucgc cuggcugcuc gggagcucca ccagccagaa ggucaucuac      540

cuggugauga uccugcucau cgcccccgcc uacuccaucc ggugcaucgg cgugagcaac      600

cgcgacuucg ucgaggggau guccggcggc accugggugg acgugguccu ggagcacggg      660

ggcugcguga ccgugauggc gcaggacaag ccgacggucg acaucgagcu ggugaccacc      720

accgugagca acauggccga gguccggucc uacugcuacg aggccagcau cagcgacaug      780

gccuccgaca gccgcugccc cacgcagggg gaggccuacc ucgacaagca guccgacacc      840

caguacgugu gcaagcggac ccuggucgac cgcggcuggg gcaacgggug cggccuguuc      900

gggaagggca gccucgugac cugcgcgaag uucacgugcu ccaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguaccgg aucaugcugu ccgugcacgg cagccagcac     1020

uccggcauga ucgucaacga cgagaaccgc gccaaggugg aggugacccc caacagcccg     1080

cgggccgagg ccacccucgg gggcuucggg ucccugggcc uggacugcga gccccgcacg     1140

gggcucgacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccugccc uggcacuccg gcgccgacac cgagaccccg     1260

cacuggaaca acaaggaggc gcugguggag uucaaggacg cccacgccaa gcggcagacg     1320

guggucgugc ucggcagcca ggagggggcc gugcacaccg cccuggcggg cgcccuggag     1380

gccgagaugg acggggccaa gggccgccuc uccagcgggc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggcgucucc uacagccugu gcaccgcggc cuucaccuuc     1500

acgaaggugc ccgccgagac ccuccacggc accgugaccg ucgaggugca guacgccggg     1560

cgcgacggcc ccugcaaggu gcccgcccag auggcggucg acaugcagac ccugacgccg     1620

guggggcggc ugaucaccgc caaccccgug aucaccgagu ccaccgagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac agcuacaucg ucaucggcgu gggggacaag     1740

aagaucacgc accacuggca ccgcuccggc agcaucaucg ggaaggccuu cgaggccacc     1800

gugcggggcg ccaagcgcau ggcgguccug ggggacaccg ccugggacuu cggcuccgug     1860

ggcggggugu ucaacagccu cggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

ucccuguucg ggggcaugag cugguucucc cagauccuga ucggcacccu gcucgucugg     1980

cuggggcuga acacgaagaa cggcagcauc ucccugaccu gccuggcccu cgggggcgug     2040

augaucuucc ucagcaccgc gguguccgcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2220

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2275


<210>  3525
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 1 GC

<400>  3525
gggagaaagc uuaccauggg cgccgacacc agcaucggga ucaucggccu gcuccugacc       60

acggccaugg cggccgagau cacccgccgg ggguccgccu acuacaugua ccuggaccgc      120

agcgacgccg gcaaggccau cuccuucgcg accaccaugg gggugaacaa gugccacguc      180

cagaucaugg accucggcca caugugcgac gccacgauga gcuacgagug ccccaugcug      240

gacgagggcg uggagccgga cgacguggac ugcuggugca acaccaccuc caccuggguc      300

guguacggga cgugccacca caagaagggc gaggcccggc ccagccgccg ggccgugacc      360

cugcccuccc acagcacccg caagcuccag acccgguccc agacguggcu ggagagccgc      420

gaguacacca agcaccugau caaggucgag aacuggaucc uccggaaccc cggguucacc      480

guggcggccc uggccauggc cuggcugcuc ggcaacucca ccagccagcg cgugaucuac      540

cuggucauga uccugcucau cgccccggcg uacuccaucc ggugcaucgg ggugagcaac      600

cgcgacuucg uggagggcau guccggcggg acgugggucg acguggugcu ggagcacggc      660

gggugcguca ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggucaccacg      720

accgugagca acauggccga ggugcggucc uacugcuacg aggccagcau cuccgacaug      780

gccagcgaca gccgcugccc cacccagggc gaggcguacc ucgacaagca guccgacacc      840

caguacgucu gcaagcggac gcugguggac cgcggcuggg ggaacggcug cgggcuguuc      900

ggcaagggga gccucgugac cugcgccaag uucacgugcu ccaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguaccgg aucaugcugu ccguccacgg cagccagcac     1020

uccgggauga ucgugaacga caucggccac gagaccgacg agaaccgcgc caaggucgag     1080

gugaccccga acagcccccg ggccgaggcc acccucgggg gcuucggguc ccugggccug     1140

gacugcgagc cccgcaccgg ccucgacuuc agcgaccugu acuaccugac gaugaacaac     1200

aagcacuggc ucgugcacaa ggagugguuc cacgacaucc cccugccgug gcacgcgggg     1260

gccgacaccg gcacgcccca cuggaacaac aaggaggccc uggucgaguu caaggacgcc     1320

cacgccaagc ggcagaccgu ggugguccuc gggucccagg agggcgcggu gcacaccgcc     1380

cuggccgggg cccuggaggc ggagauggac ggcgccaagg gccgccucuu cagcgggcac     1440

cugaagugcc gcaucaagau ggacaagcug cggcucaagg gcguguccua cagccugugc     1500

accgccgccu ucaccuucuc caaggucccc gccgagaccc ugcacgggac cgugacggug     1560

gagguccagu acgcgggccg cgacggcccc ugcaaggugc cggcccagau ggccguggac     1620

augcagaccc ucacccccgu cgggcggcug aucaccgcca accccaucau cacggagagc     1680

accgagaacu cccgcaugau gcuggagcuc gacccgcccu ucggcgacag cuacaucgug     1740

aucggggugg gcgacaagaa gaucacccac cacuggcacc gguccgggag caccaucggc     1800

aaggccuucg aggcgacggu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcc     1860

ugggacuucg gcagcgucgg gggcguguuc aacucccugg ggaagggcgu gcaccaggug     1920

uucggcgccg cguucaagag ccucuucggg ggcauguccu gguucagcca gauccugauc     1980

gggacccugc uggucuggcu cggccugaac accaagaacg gguccaucag ccuggccugc     2040

cucgcccugg gcggcgccau gaucuuccug uccacggcgg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3526
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 1 GC

<400>  3526
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      180

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      360

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug      840

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1080

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1140

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3527
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 1 GC

<400>  3527
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3528
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 1 GC

<400>  3528
gggagaaagc uuaccauguc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc       60

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      120

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      180

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      300

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      420

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg      540

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug      600

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac      660

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc      840

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg      900

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug      960

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1080

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1140

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1200

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1260

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1320

auggccgugc ucggggacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3529
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 1 GC

<400>  3529
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc      840

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3530
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 1 GC

<400>  3530
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggcuacgaga ccgacgagaa ccgggccaag guggagguca cgcccaacuc cccgcgcgcc      600

gaggccaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acacgggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggcaa gcuguucagc gggcaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggcgu guccuacagc cucugcacgg ccgccuucac cuucaccaag     1020

guccccgccg agacccugca cgggacggug accguggagg uccaguacgc cggcaccgac     1080

ggccccugca agauccccgu gcagauggcg guggacaugc agacccugac gccggucggg     1140

cggcucauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1260

acgcaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc caccgugcgg     1320

ggcgccaagc gcauggcggu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3531
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 1 GC

<400>  3531
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaccgu gaacgacauc      540

ggcuacgaga ccgacgagaa ccgggccaag guggagguca cgcccaacuc cccgcgcgcc      600

gaggccaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acacgggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggcaa gcuguucagc gggcaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggcgu guccuacagc cucugcacgg ccgccuucac cuucaccaag     1020

guccccgccg agacccugca cgggacggug accguggagg uccaguacgc cggcaccgac     1080

ggccccugca agauccccgu gcagauggcg guggacaugc agacccugac gccggucggg     1140

cggcucauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1260

acgcaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc caccgugcgg     1320

ggcgccaagc gcauggcggu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3532
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 1 GC

<400>  3532
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag      540

aaccgggcca agguggaggu cacccccaac uccccgcgcg ccgaggccac ccugggcggg      600

uucggcagcc ugggccucga cugcgagccc cggacggggc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagacggucg uggugcuggg cagccaggag      840

ggggcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg cgcgaagggg      900

cggcuguuca gcggccaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggg      960

guguccuaca gccucugcac cgccgccuuc accuucacga agguccccgc cgagacccug     1020

cacggcaccg ugaccgugga gguccaguac gccgggacgg acggccccug caaggugccc     1080

gcgcagaugg ccguggacau gcagacccug accccggucg gccgccucau caccgccaac     1140

cccgugauca cggaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggggacuccu acaucgugau cggcgucggg gacaagaaga ucacccacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccacggugc gcggcgcgaa gcggauggcc     1320

gugcucggcg acaccgccug ggacuucggg agcugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  3533
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 1 GC

<400>  3533
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgg cuacgagacc      540

gacgagaacc gggccaaggu ggaggugacc cccaacuccc cgcgcgccga ggccacgcuc      600

gggggcuucg gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac      660

cuguacuacc ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc gggggccgac accggcacgc cgcacuggaa caacaaggag      780

gcccucgugg aguucaagga cgcccacgcc aagcgccaga ccguggucgu gcuggggagc      840

caggagggcg cggugcacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg      900

aagggcaagc uguucagcgg gcaccugaag ugccggcuca agauggacaa gcugcgccug      960

aagggcgucu ccuacagccu cugcaccgcc gccuucacgu ucaccaaggu gcccgccgag     1020

acccugcacg ggaccgucac gguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggcggu ggacaugcag acccugaccc cgguggggcg gcucaucacg     1140

gccaaccccg ucaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg     1200

cccuucggcg acuccuacau cgugaucggg gugggcgaca agaagaucac ccaccacugg     1260

caccgcagcg gguccacgau cggcaaggcc uucgaggcca ccguccgggg cgccaagcgc     1320

auggcggugc ucggggacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3534
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 1 GC

<400>  3534
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu cccugcgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcucgag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcugguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcuggacu gcgagccccg gaccggccug      660

gacuucuccg accucuacua ccugaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccucg ccggcgcccu ggaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuca agugccgccu gaagauggac      960

aagcugcggc ucaagggcgu cagcuacucc cugugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccucac cccggugggg     1140

cgccugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcucgacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3535
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 1 GC

<400>  3535
gggagaaagc uuaccaugag caagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3536
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 1 GC

<400>  3536
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu gguaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3537
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 1 GC

<400>  3537
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggcaa gcugagcucc gggcaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgg     1320

ggcgccaagc gcauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3538
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 1 GC

<400>  3538
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaccc agacccugac gccggugggg     1140

cgccucauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acgcaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3539
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 1 GC

<400>  3539
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgagauc      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg cgaagcgcca gaccguggug      840

guccugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg uccaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3540
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 1 GC

<400>  3540
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgcccggc gcauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3541
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 1 GC

<400>  3541
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacaugguca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gccaaguucg cgugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag gucgagauca cccccaacuc cccgcgcgcc      600

gaggccacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1080

ggccccugca agguccccgc gcagauggcc guggacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc caccguccgc     1320

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3542
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 1 GC

<400>  3542
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaucg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3543
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 1 GC

<400>  3543
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg ggaucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3544
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 1 GC

<400>  3544
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cuggccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcucgag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcugguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcc      600

gaggcgacgc ucgggggcuu cggcucccug gggcuggacu gcgagccgcg gaccggccug      660

gacuucuccg accucuacua ccugaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggggccg acaccggcac gccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccucg ccggcgcccu ggaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuca agugccgccu gaagauggac      960

aagcugcggc ucaagggcgu cagcuacucc cugugcaccg ccgccuucac guucaccaag     1020

aucccggccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccucac ccccgugggg     1140

cgccugauca cggccaaccc ggugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcucgacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3545
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 1 GC

<400>  3545
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga cccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cuccuacagc cucugcacgg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cgggacggug accgucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac gccggugggg     1140

cgccucauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1260

acgcaccacu ggcaccggag cggguccacc aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3546
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 1 GC

<400>  3546
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcuggc ccagauggcc gucgacaugc agacccucac ccccgugggg     1140

cgccugauca cggccaaccc ggugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcucgacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3547
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 1 GC

<400>  3547
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gaggcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3548
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 1 GC

<400>  3548
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcggccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gacgggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcgcu cgaggccgag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac cuucacgaag     1020

auccccgcgg agacccugca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg cguucgaggc cacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3549
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 1 GC

<400>  3549
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc agcaccaccg uguccaacau ggccgaggug      240

cgcagcuacu gcuacgaggc caccaucucc gacaucgcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgccgug      840

guccugggga gccaggaggg cgccgugcac accgcccugg ccggcgcgcu cgaggccgag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgcgg agacccugca cgggaccguc acgguggagg uccaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg cguucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3550
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 1 GC

<400>  3550
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucagcaag     1020

auccccgccg agacccugca cgggaccgug accgaggagg uccaguacgc gggcacggac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca ccgccaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccggag cggguccacc aucggcaagg ccuucgaggc gacggugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3551
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 1 GC

<400>  3551
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagc ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccucac cccggucggg     1140

cgccugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcucgacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3552
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 1 GC

<400>  3552
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcauc agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugaccgu cauggcgcag      180

gacaagccca cgguggacau cgagcucgug accaccaccg ucagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcauccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg cgaagcgcca gaccguggug      840

guccuggggu cccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu guccuacagc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg uccaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccggag cggguccacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3553
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 1 GC

<400>  3553
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ucaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1080

ggccccugca agguccccgc gcagauggcc guggacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc caccguccgc     1320

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3554
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 1 GC

<400>  3554
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcgccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg cguucgaggc cacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3555
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 1 GC

<400>  3555
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac accggggccg acacgggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgcggugcac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcacgg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc gcagauggcc gucgacaugc agacccugac gccggugggg     1140

cgccucauca ccgccaaccc cgugaucacc gagggcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg ggacuccuac aucgucaucg gcguggggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggggcgaagc ggauggccgu ccucggcgac accgccuggg acuucgggag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3556
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 1 GC

<400>  3556
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgc gauggcccag      180

gacaagccca cgguggacau cgagcucgug accaccaccg ucagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg cguaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu gcacggcucc cagcacagcg ggaugcuggu caacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcg      600

gaggccacgc ucgggggcuu cggcagccuc gggcuggacu gcgagccccg gaccggccug      660

gacuucuccg accucuacua ccugaccaug aacaacaagc acuggcuggc ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggggccg cgaccggcac gccgcacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgccguccac accgcgcucg ccggcgcccu ggaggccgag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuca agugccgccu gaagauggac      960

aagcugcggc ucaagggcgu gagcuacucc cugugcaccg cggccuucac guucaccaag     1020

auccccgccg agaccgugga cgggaccguc acgguggagg gccaguacgg cgggaccgac     1080

ggccccugca agguccccgc ccagauggcc guggacaugc agacccugac cccggugggg     1140

cgccucauca cggcgaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cggcagcacg aucgggaagg ccuucgaggc caccguccgc     1320

ggcgccaagc ggauggcggu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3557
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 1 GC

<400>  3557
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagcccg ccguggacau cgagcucguc acgaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      300

cagggcgagg cguaccugga caagcagagc gacacgcagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacg      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcg      600

gaggccaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gacgggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcgcu cgaggccgag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac cuucacgaag     1020

auccccgcgg agacccugca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg cguucgaggc cacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3558
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 1 GC

<400>  3558
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug aacaaccggg acuucgugga ggggaugagc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg uguccaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacg      300

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugaccugc      420

gcgaaguucg ccugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcuggggu cccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cuccuacagc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccggag cggguccacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3559
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 1 GC

<400>  3559
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcgccg ccgccuucac cuucacgaag     1020

auccccgcgg agacccugca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg cguucgaggc cacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3560
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 1 GC

<400>  3560
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gacgggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gacggugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac cuucacgaag     1020

auccccgccg agacccugca cgggaccgug accgucgagg ugcaguacgc gggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3561
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 1 GC

<400>  3561
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1260

acccaccacu gguaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3562
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 1 GC

<400>  3562
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagguca cgcccaacuc cccgcgcgcc      600

gaggccaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acacgggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcuguucagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu guccuacagc cucugcacgg ccgccuucac cuucaccaag     1020

guccccgccg agacccugca cgggacggug accguggagg uccaguacgc cggcaccgac     1080

ggccccugca aggugcccgc gcagauggcc guggacaugc agacccugac gccggucggg     1140

cgccucauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1260

acgcaccacu ggcaccggag cggguccacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3563
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 1 GC

<400>  3563
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa ccgggccaag guggagguca cccccaacuc cccgcgcgcc      600

gaggccacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcuguucagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu guccuacagc cucugcaccg ccgccuucac guucaccaag     1020

guccccgccg agacccugca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1080

ggccccugca aggugcccgc gcagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1260

acccaccacu ggcaccggag cggguccacg aucggcaagg ccuucgaggc caccgugcgc     1320

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3564
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 1 GC

<400>  3564
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa ccgggccaag guggagguca cccccaacuc cccgcgcgcc      600

gaggccacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcuguucagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu guccuacagc cucugcaccg ccgucugcac ggccgccaag     1020

gugcccgccg agacccugca cgggaccgug accgucgagg ugcaguacgc gggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga caagaagauc     1260

acccaccacu ggcaccggag cggguccacc aucggcaagg ccuucgaggc gacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3565
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 1 GC

<400>  3565
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag      540

aaccgggcca agguggaggu cacccccaac uccccgcgcg ccgaggccac ccugggcggg      600

uucggcagcc ugggccucga cugcgagccc cggacggggc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acagcggcgc ggacaccgag accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagacggucg uggugcuggg gagccaggag      840

ggcgccgucc acaccgcgcu ggccggggcc cucgaggccg agauggacgg cgccaagggc      900

cggcugucca gcgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccucugcac cgcggccuuc accuucacga agguccccgc cgagacccug     1020

cacgggaccg ugaccgugga gguccaguac gccggccgcg acgggcccug caaggugccc     1080

gcccagaugg cgguggacau gcagacgcug accccggucg gccggcucau caccgccaac     1140

cccgugauca ccgaguccac ggagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggggucggc gacaagaaga ucacccacca cuggcaccgc     1260

agcgggucca ucaucggcaa ggccuucgag gccaccgugc gcggggccaa gcggauggcg     1320

gugcucggcg acaccgccug ggacuucggc agcugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  3566
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 1 GC

<400>  3566
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugaucgu gcucaucgug       60

cccgccuacu ccaucagcug caucggcguc uccaaccgcg accuggugga ggggaugagc      120

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugaccga gauggcccag      180

gacaagccca cggucgacau cgagcucgug accaugaccg uguccaacau ggcggagguc      240

cggagcuacu gcuacgaggc cucccugagc gacauggccu ccgccagccg cugccccacc      300

cagggcgagc ccucccugga caagcagagc gacacgcagu ccgugugcaa gcggacccuc      360

ggcgaccgcg gguggggcaa cgggugcggc aucuucggga agggcagccu ggugaccugc      420

uccaaguuca cgugcugcaa gaagaugccc ggcaagagca uccagccgga gaaccuggag      480

uaccggauca ugcuccccgu ccacgggucc cagcacagcg gcaugaucgu gaacgacauc      540

gggcacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacag cccccgggcg      600

gaggccaccc ugggcggcuu cgggucccug ggccucgacu gcgagccgcg caccgggcug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccgggac gccccacugg      780

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug      840

gugcugggcu cccaggaggg cgccguccac accgcccugg cgggggcccu cgaggccgag      900

auggacggcg ccaaggggcg ccuguucagc ggccaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggggu guccuacagc cucugcaccg ccgcguucac cuucaccaag     1020

gucccggccg agacgcugca cggcaccgug accguggagg uccaguccgc cggcaccgac     1080

gggcccugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggc     1140

cggcucauca ccgcgaaccc ggugaucacc gagagcacgg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg ggacagcuac aucgugaucg gcgucgggga caagaagauc     1260

acccaccacu ggcaccgcuc cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggggccaagc ggauggccgu gcucggcgac acggcguggg acuucggguc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3567
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 1 GC

<400>  3567
gggagaaagc uuaccaugcg ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuaca gcauccggug caucggcgug uccaaccgcg acuucgugga ggggaugagc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg uguccaacau ggccgaggug      240

cggagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg cugccccacg      300

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcggacccug      360

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugaccugc      420

gcgaaguucg ccugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag      480

uaccggauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccgcgggcc      600

gaggcgacgc ucgggggcuu cggcucccug gggcucgacu gcgagccccg caccggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcggca gaccgugguc      840

gugcuggggu cccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg ccugagcagc gggcaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggcgu cuccuacagc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cggcucauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc gaccgugcgg     1320

ggcgccaagc gcauggccgu ccucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3568
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 1 GC

<400>  3568
gggagaaagc uuaccaugag ccagcgcgug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccggug caucggcgug agcaaccgcg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgguccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg cugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcggacccug      360

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccggauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacuc cccgcgggcc      600

gaggccacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg caccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg ccuguucagc gggcaccuga agugccggau caagauggac      960

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgccuucac guucuccaag     1020

guccccgccg agacccucca cgggaccgug accguggagg uccaguacgc cggccgggac     1080

ggccccugca aggugcccgc gcagauggcc guggacaugc agacgcugac cccggucggg     1140

cgccugauca ccgccaaccc caucaucacc gagagcacgg agaacucccg gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1260

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc caccguccgg     1320

ggcgcgaagc gcauggccgu gcugggggac acggccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3569
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  3569
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3570
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 opt

<400>  3570
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacacc      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacgcca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3571
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 opt

<400>  3571
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc     1080

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     2040

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcuccguc gacuucucca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3572
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 1 opt

<400>  3572
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3573
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  3573
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3574
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 1 opt

<400>  3574
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3575
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 1 opt

<400>  3575
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3576
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 1 opt

<400>  3576
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3577
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 opt

<400>  3577
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3578
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 opt

<400>  3578
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3579
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 opt

<400>  3579
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc      540

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc      600

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      660

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      720

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc      840

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc      900

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc      960

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1020

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1140

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1200

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1260

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1320

auggccgucc ucggcgacac cgccugggac uucggcuccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3580
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 1 opt

<400>  3580
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3581
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       1 opt

<400>  3581
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuc cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3582
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 1
       opt

<400>  3582
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuc cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3583
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 1 
       opt

<400>  3583
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuc cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3584
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 1 
       opt

<400>  3584
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuc cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3585
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       1 opt

<400>  3585
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucguccagg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3586
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 1
       opt

<400>  3586
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucguccagg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3587
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 1 
       opt

<400>  3587
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucguccagg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3588
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  3588
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3589
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  3589
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3590
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 1 opt

<400>  3590
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc     1800

aagggcgccc agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc     1860

ggcgucuuca acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc     1920

cucuucggcg gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug     1980

ggcgucaacg cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc     2040

gucuuccucg ccaccaacgu ccacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3591
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  3591
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3592
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  3592
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3593
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3593
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcgccg cggcgccgac       60

accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc      120

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      180

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      240

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      300

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      360

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      420

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      480

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      540

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      600

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      660

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      720

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      780

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      840

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      900

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac     1020

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1080

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1140

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1200

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1260

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1380

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1440

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1500

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1620

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1680

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1740

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1800

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1860

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1920

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1980

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     2040

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2100

cucuccaccg ccgucuccgc cgacgucggc ugcuccgucg acuucuccaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  3594
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3594
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcgccgcgg cgccgacacc       60

uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc      120

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      180

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      240

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      300

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      360

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      420

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      480

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      540

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      600

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      660

accugggucg acaucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      720

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      780

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      840

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      900

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      960

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc     1020

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1080

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1140

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1200

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1260

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1380

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1440

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1500

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1620

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1680

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1740

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1800

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1920

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1980

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     2040

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2100

uccaccgccg ucuccgccga cgucggcugc uccgucgacu ucuccaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  3595
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3595
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc gccgcggcgc cgacaccucc       60

gucggcaucg ucggccuccu ccucaccacc gccauggccg ccgaggucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcucc gucgacuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3596
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3596
gggagaaagc uuaccauggc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc       60

ggcaucgucg gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc      120

gccuacuaca uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc      180

cucggcauga acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc      240

auguccuacg agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg      300

ugcaacacca ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc      360

cgccgcuccc gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      420

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      480

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      540

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      600

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      660

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      720

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      780

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      840

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      900

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug     1020

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc     1080

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     2040

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcuccguc gacuucucca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3597
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3597
gggagaaagc uuaccaugcg caaggagaag aagcgccgcg gcgccgacac cuccgucggc       60

aucgucggcc uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc      120

uacuacaugu accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc      180

ggcaugaaca agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc      300

aacaccaccu ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc      360

cgcucccgcc gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      480

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      540

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      600

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      660

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      780

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      840

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      900

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      960

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc     1020

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac     1080

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc     1140

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1200

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1260

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1380

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1440

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1500

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1620

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1680

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1740

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1800

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1860

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1980

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     2040

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     2100

gucuccgccg acgucggcug cuccgucgac uucuccaagu gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  3598
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3598
gggagaaagc uuaccaugaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3599
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3599
gggagaaagc uuaccaugga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgacgucg gcugcuccgu cgacuucucc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  3600
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3600
gggagaaagc uuaccaugaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gacgucggcu gcuccgucga cuucuccaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  3601
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3601
gggagaaagc uuaccaugaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugcu ccgucgacuu cuccaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3602
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3602
gggagaaagc uuaccaugcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugcuccg ucgacuucuc caagugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3603
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3603
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcuccgucg acuucuccaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3604
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3604
gggagaaagc uuaccauggg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

uccgucgacu ucuccaagug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3605
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3605
gggagaaagc uuaccauggc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgacgu cggcugcucc     2100

gucgacuucu ccaagugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3606
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3606
gggagaaagc uuaccaugga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgacgucgg cugcuccguc     2100

gacuucucca agugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3607
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3607
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cuccgucgac     2100

uucuccaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3608
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3608
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cuccgucgac     2100

uucuccaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3609
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3609
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccgc cgucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcuccgucg acuucuccaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3610
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3610
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcggcgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcucc gucgacuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3611
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3611
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcgccgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcucc gucgacuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3612
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3612
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3613
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3613
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcgccg cggcgccgac       60

accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc      120

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      180

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      240

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      300

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      360

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      420

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      480

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      540

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      600

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      660

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      720

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      780

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      840

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      900

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac     1020

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1080

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1140

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1200

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1260

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1380

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1440

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1500

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1620

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1680

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1740

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1800

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1860

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1920

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1980

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     2040

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2100

cucuccaccg ccgucuccgc cgacgucggc ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  3614
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3614
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcgccgcgg cgccgacacc       60

uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc      120

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      180

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      240

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      300

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      360

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      420

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      480

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      540

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      600

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      660

accugggucg acaucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      720

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      780

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      840

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      900

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      960

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc     1020

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1080

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1140

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1200

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1260

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1380

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1440

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1500

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1620

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1680

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1740

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1800

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1920

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1980

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     2040

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2100

uccaccgccg ucuccgccga cgucggcugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  3615
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3615
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc gccgcggcgc cgacaccucc       60

gucggcaucg ucggccuccu ccucaccacc gccauggccg ccgaggucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3616
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3616
gggagaaagc uuaccauggc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc       60

ggcaucgucg gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc      120

gccuacuaca uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc      180

cucggcauga acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc      240

auguccuacg agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg      300

ugcaacacca ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc      360

cgccgcuccc gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      420

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      480

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      540

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      600

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      660

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      720

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      780

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      840

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      900

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug     1020

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc     1080

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     2040

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3617
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3617
gggagaaagc uuaccaugcg caaggagaag aagcgccgcg gcgccgacac cuccgucggc       60

aucgucggcc uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc      120

uacuacaugu accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc      180

ggcaugaaca agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc      300

aacaccaccu ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc      360

cgcucccgcc gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      480

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      540

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      600

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      660

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      780

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      840

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      900

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      960

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc     1020

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac     1080

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc     1140

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1200

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1260

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1380

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1440

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1500

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1620

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1680

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1740

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1800

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1860

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1980

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     2040

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     2100

gucuccgccg acgucggcug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3618
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3618
gggagaaagc uuaccaugaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3619
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3619
gggagaaagc uuaccaugga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgacgucg gcugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3620
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3620
gggagaaagc uuaccaugaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gacgucggcu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3621
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3621
gggagaaagc uuaccaugaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3622
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3622
gggagaaagc uuaccaugcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3623
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3623
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3624
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3624
gggagaaagc uuaccauggg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3625
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3625
gggagaaagc uuaccauggc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgacgu cggcugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3626
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3626
gggagaaagc uuaccaugga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgacgucgg cugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3627
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3627
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3628
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3628
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3629
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3629
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccgc cgucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3630
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3630
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcggcgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3631
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3631
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcgccgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3632
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3632
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3633
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3633
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcgccg cggcgccgac       60

accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc      120

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      180

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      240

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      300

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      360

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      420

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      480

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      540

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      600

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      660

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      720

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      780

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      840

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      900

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac     1020

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1080

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1140

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1200

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1260

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1380

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1440

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1500

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1620

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1680

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1740

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1800

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1860

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1920

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1980

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     2040

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2100

cucuccaccg ccgucuccgc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3634
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3634
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcgccgcgg cgccgacacc       60

uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc      120

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      180

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      240

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      300

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      360

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      420

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      480

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      540

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      600

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      660

accugggucg acaucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      720

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      780

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      840

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      900

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      960

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc     1020

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1080

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1140

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1200

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1260

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1380

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1440

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1500

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1620

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1680

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1740

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1800

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1920

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1980

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     2040

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2100

uccaccgccg ucuccgccga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3635
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3635
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc gccgcggcgc cgacaccucc       60

gucggcaucg ucggccuccu ccucaccacc gccauggccg ccgaggucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3636
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3636
gggagaaagc uuaccauggc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc       60

ggcaucgucg gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc      120

gccuacuaca uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc      180

cucggcauga acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc      240

auguccuacg agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg      300

ugcaacacca ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc      360

cgccgcuccc gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      420

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      480

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      540

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      600

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      660

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      720

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      780

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      840

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      900

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug     1020

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc     1080

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     2040

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     2100

gccgucuccg ccgacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3637
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3637
gggagaaagc uuaccaugcg caaggagaag aagcgccgcg gcgccgacac cuccgucggc       60

aucgucggcc uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc      120

uacuacaugu accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc      180

ggcaugaaca agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc      300

aacaccaccu ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc      360

cgcucccgcc gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      480

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      540

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      600

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      660

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      780

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      840

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      900

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      960

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc     1020

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac     1080

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc     1140

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1200

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1260

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1380

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1440

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1500

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1620

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1680

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1740

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1800

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1860

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1980

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     2040

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     2100

gucuccgccg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3638
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3638
gggagaaagc uuaccaugaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3639
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3639
gggagaaagc uuaccaugga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3640
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3640
gggagaaagc uuaccaugaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3641
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3641
gggagaaagc uuaccaugaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3642
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3642
gggagaaagc uuaccaugcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3643
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3643
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3644
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3644
gggagaaagc uuaccauggg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3645
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3645
gggagaaagc uuaccauggc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3646
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3646
gggagaaagc uuaccaugga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  3647
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3647
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3648
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3648
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3649
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3649
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccgc cgucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3650
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3650
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcggcgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3651
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3651
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcgccgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3652
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3652
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3653
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3653
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcgccgcgg cgccgacacc       60

uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc      120

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      180

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      240

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      300

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      360

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      420

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      480

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      540

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      600

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      660

accugggucg acaucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      720

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      780

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      840

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      900

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      960

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc     1020

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1080

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1140

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1200

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1260

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1380

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1440

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1500

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1620

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1680

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1740

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1800

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1920

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1980

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     2040

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2100

uccaccgccg ucuccgccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3654
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3654
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcgccg cggcgccgac       60

accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc      120

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      180

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      240

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      300

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      360

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      420

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      480

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      540

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      600

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      660

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      720

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      780

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      840

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      900

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac     1020

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1080

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1140

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1200

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1260

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1380

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1440

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1500

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1620

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1680

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1740

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1800

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1860

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1920

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1980

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     2040

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2100

cucuccaccg ccgucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3655
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3655
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc gccgcggcgc cgacaccucc       60

gucggcaucg ucggccuccu ccucaccacc gccauggccg ccgaggucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3656
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3656
gggagaaagc uuaccauggc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc       60

ggcaucgucg gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc      120

gccuacuaca uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc      180

cucggcauga acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc      240

auguccuacg agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg      300

ugcaacacca ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc      360

cgccgcuccc gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      420

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      480

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      540

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      600

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      660

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      720

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      780

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      840

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      900

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug     1020

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc     1080

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     2040

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     2100

gccgucuccg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3657
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3657
gggagaaagc uuaccaugcg caaggagaag aagcgccgcg gcgccgacac cuccgucggc       60

aucgucggcc uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc      120

uacuacaugu accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc      180

ggcaugaaca agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc      300

aacaccaccu ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc      360

cgcucccgcc gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      480

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      540

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      600

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      660

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      780

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      840

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      900

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      960

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc     1020

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac     1080

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc     1140

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1200

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1260

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1380

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1440

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1500

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1620

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1680

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1740

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1800

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1860

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1980

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     2040

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     2100

gucuccgccu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3658
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3658
gggagaaagc uuaccaugaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3659
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3659
gggagaaagc uuaccaugga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3660
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3660
gggagaaagc uuaccaugaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3661
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3661
gggagaaagc uuaccaugaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3662
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3662
gggagaaagc uuaccaugcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3663
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3663
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3664
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3664
gggagaaagc uuaccauggg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3665
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3665
gggagaaagc uuaccauggc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  3666
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3666
gggagaaagc uuaccaugga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3667
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  3667
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3668
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3668
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3669
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3669
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccgc cgucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3670
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3670
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcggcgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3671
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3671
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcgccgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3672
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 1 opt

<400>  3672
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3673
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 1 opt

<400>  3673
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacacc      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacgcca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3674
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 1 opt

<400>  3674
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc     1080

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     2040

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcuccguc gacuucucca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3675
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 1 opt

<400>  3675
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3676
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 1 opt

<400>  3676
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3677
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 1 opt

<400>  3677
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucacccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3678
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 1 opt

<400>  3678
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccgcaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3679
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 1 opt

<400>  3679
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaccgu caacgacauc     1080

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3680
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 1 opt

<400>  3680
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc     1080

gacgagaacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggcaagc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     2040

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcuccguc gacuucucca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3681
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 1 opt

<400>  3681
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggccg cggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3682
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 1 opt

<400>  3682
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacgccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3683
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 1 opt

<400>  3683
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc cucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3684
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 1 opt

<400>  3684
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu gguaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3685
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 1 opt

<400>  3685
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagauc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3686
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 1 opt

<400>  3686
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccucgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3687
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 1 opt

<400>  3687
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc acccucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3688
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 1 opt

<400>  3688
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgucgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3689
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 1 opt

<400>  3689
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccgcc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcccccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3690
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 1 opt

<400>  3690
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc caagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3691
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 1 opt

<400>  3691
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccacccg ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3692
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 1 opt

<400>  3692
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcuccgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaccc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3693
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 1 opt

<400>  3693
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccaacg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgcccgcc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3694
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 1 opt

<400>  3694
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gagaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3695
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 1 opt

<400>  3695
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu cccuccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3696
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 1 opt

<400>  3696
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccaacg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggauguuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3697
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 1 opt

<400>  3697
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggauccuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcaucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3698
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 1 opt

<400>  3698
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc uccaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc caccaucucc gacaucgccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgccguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3699
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 1 opt

<400>  3699
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggca cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3700
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 1 opt

<400>  3700
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagc uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3701
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 1 opt

<400>  3701
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gagaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

aucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3702
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 1 opt

<400>  3702
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaucg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3703
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 1 opt

<400>  3703
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ucaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucuccuuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3704
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 1 opt

<400>  3704
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggcaa gcucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3705
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 1 opt

<400>  3705
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gaggcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3706
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 1 opt

<400>  3706
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcgccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3707
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 1 opt

<400>  3707
gggagaaagc uuaccaugcg caucaucaac gccggcaagg agaagaagcg ccgcggcgcc       60

gacaccaacg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgcccgcc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3708
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 1 opt

<400>  3708
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gagaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaagg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3709
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 1 opt

<400>  3709
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggucu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3710
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 1 opt

<400>  3710
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccaacg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacau gaccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3711
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 1 opt

<400>  3711
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccgccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagacc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3712
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 1 opt

<400>  3712
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gagaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cucgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3713
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 1 opt

<400>  3713
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcauc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcauccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3714
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 1 opt

<400>  3714
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacacc      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cauccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacgcca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3715
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 1 opt

<400>  3715
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccagcgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3716
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 1 opt

<400>  3716
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccaggccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3717
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 1 opt

<400>  3717
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3718
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 1 opt

<400>  3718
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac accggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gagggcaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

guccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3719
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 1 opt

<400>  3719
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgc cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugcucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgc ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg ccaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agaccgucga cggcaccguc accgucgagg gccaguacgg cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucaucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cggcggcguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3720
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 1 opt

<400>  3720
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucuccaag     1560

auccccgccg agacccucca cggcaccguc accgaggagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3721
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 1 opt

<400>  3721
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3722
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 1 opt

<400>  3722
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggca acgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca ccaugggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3723
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 1 opt

<400>  3723
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc gucugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3724
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 1 opt

<400>  3724
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagcccg ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3725
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 1 opt

<400>  3725
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgu caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3726
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 1 opt

<400>  3726
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcgu caacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu gguaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3727
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 1 opt

<400>  3727
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uuccccacca cccucggcgu caacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3728
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 1 opt

<400>  3728
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucacccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3729
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 1 opt

<400>  3729
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca accucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcguccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3730
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 1 opt

<400>  3730
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3731
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 1 opt

<400>  3731
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca accucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3732
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 1 opt

<400>  3732
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3733
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 1 opt

<400>  3733
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccagcacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgucugcac cgccgccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3734
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 1 opt

<400>  3734
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacgccucca ucggcaucgu cucccuccuc cucaccaccg ucauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgucacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acuccggcgc cgacaccgag accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggccgcg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ucaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3735
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 1 opt

<400>  3735
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3736
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 1 opt

<400>  3736
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgaga cccgccgcuc ccgccgcucc gucucccucc gcuaccacua cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaagaagca ccucaucaug      480

gucgagaacu ggaucuuccg caaccccggc uucgccaucg ucuccgucgc caucaccugg      540

cucaugggcu cccucaccuc ccagaagguc aucuaccucg ucaugaucgu ccucaucguc      600

cccgccuacu ccaucuccug caucggcguc uccaaccgcg accucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccga gauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaugaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cucccucucc gacauggccu ccgccucccg cugccccacc      840

cagggcgagc ccucccucga caagcagucc gacacccagu ccgucugcaa gcgcacccuc      900

ggcgaccgcg gcuggggcaa cggcugcggc aucuucggca agggcucccu cgucaccugc      960

uccaaguuca ccugcugcaa gaagaugccc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcuccccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguccgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3737
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 1 opt

<400>  3737
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca ccaugggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccccuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggauccuccg caaccccggc uucaccgucg ccgcccucgc cauggccugg      540

cuccucggca acuccaccuc ccagcgcguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgcau caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucuccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggccgcgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc caucaucacc gaguccaccg agaacucccg caugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcguccac caggucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc gccugccucg cccucggcgg cgccaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  3738
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 1 opt

<400>  3738
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3739
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 1 opt

<400>  3739
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3740
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 1 opt

<400>  3740
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3741
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 1 opt

<400>  3741
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3742
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 1 opt

<400>  3742
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcua cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggcaagcucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagauc cccguccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3743
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 1 opt

<400>  3743
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc gcaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcua cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggcaagcucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagauc cccguccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3744
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 1 opt

<400>  3744
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

accgucaacg acaucggcua cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggcaagcucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagauc cccguccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3745
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 1 opt

<400>  3745
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucac ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3746
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 1 opt

<400>  3746
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg caagcucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3747
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 1 opt

<400>  3747
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggccgcggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3748
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 1 opt

<400>  3748
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggccucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3749
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 1 opt

<400>  3749
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacugguac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3750
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 1 opt

<400>  3750
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga cacccagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3751
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 1 opt

<400>  3751
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agauccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3752
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 1 opt

<400>  3752
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cucgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3753
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 1 opt

<400>  3753
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccacccu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3754
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 1 opt

<400>  3754
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccguc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3755
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 1 opt

<400>  3755
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccgccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcccc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3756
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 1 opt

<400>  3756
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accuccaaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3757
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 1 opt

<400>  3757
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc acccgccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3758
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 1 opt

<400>  3758
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcacccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3759
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 1 opt

<400>  3759
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacucccuc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3760
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 1 opt

<400>  3760
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcaucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3761
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 1 opt

<400>  3761
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc cuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcauc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3762
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 1 opt

<400>  3762
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucuccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccacca ucuccgacau cgccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ccgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3763
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 1 opt

<400>  3763
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagcuccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3764
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 1 opt

<400>  3764
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caucgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3765
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 1 opt

<400>  3765
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgucaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu ccuucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3766
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 1 opt

<400>  3766
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggcaagcucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3767
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 1 opt

<400>  3767
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caaggacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3768
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 1 opt

<400>  3768
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggaggc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3769
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 1 opt

<400>  3769
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cgcccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agacccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3770
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 1 opt

<400>  3770
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggcg ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3771
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 1 opt

<400>  3771
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccucgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3772
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 1 opt

<400>  3772
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggucuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3773
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 1 opt

<400>  3773
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcaucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggca uccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3774
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 1 opt

<400>  3774
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaucc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3775
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 1 opt

<400>  3775
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccag cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3776
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 1 opt

<400>  3776
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca ggcccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3777
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 1 opt

<400>  3777
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuucu ccaagauccc cgccgagacc cuccacggca ccgucaccga ggagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3778
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 1 opt

<400>  3778
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacaccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaggg caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcguccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3779
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 1 opt

<400>  3779
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3780
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 1 opt

<400>  3780
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcaacgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccaccaug ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3781
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 1 opt

<400>  3781
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucgucug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3782
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 1 opt

<400>  3782
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucaacaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucgucug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3783
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 1 opt

<400>  3783
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccgccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3784
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 1 opt

<400>  3784
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgucaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3785
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 1 opt

<400>  3785
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcgucaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacugguac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3786
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 1 opt

<400>  3786
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuuccc caccacccuc ggcgucaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3787
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 1 opt

<400>  3787
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucac ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3788
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 1 opt

<400>  3788
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccaaccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggcg uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3789
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 1 opt

<400>  3789
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3790
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 1 opt

<400>  3790
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccaaccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3791
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 1 opt

<400>  3791
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3792
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 1 opt

<400>  3792
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccagc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccguc     1500

ugcaccgccg ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3793
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 1 opt

<400>  3793
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  3794
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 1 opt

<400>  3794
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgagacccgc cgcucccgcc gcuccgucuc ccuccgcuac      360

cacuacaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacaag      420

aagcaccuca ucauggucga gaacuggauc uuccgcaacc ccggcuucgc caucgucucc      480

gucgccauca ccuggcucau gggcucccuc accucccaga aggucaucua ccucgucaug      540

aucguccuca ucguccccgc cuacuccauc uccugcaucg gcgucuccaa ccgcgaccuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgagaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccau gaccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccuccc ucuccgacau ggccuccgcc      780

ucccgcugcc ccacccaggg cgagcccucc cucgacaagc aguccgacac ccaguccguc      840

ugcaagcgca cccucggcga ccgcggcugg ggcaacggcu gcggcaucuu cggcaagggc      900

ucccucguca ccugcuccaa guucaccugc ugcaagaaga ugcccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc cccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uccgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3795
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 1 opt

<400>  3795
gggagaaagc uuaccauguc cgucggcauc gucggccucc uccucaccac cgccauggcc       60

gccgagguca cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc      120

gaggccaucu ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac      180

cucggccaca ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc      240

gagcccgacg acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc      300

ugccaccaca agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac      360

uccacccgca agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag      420

caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc      480

gccaucgccu ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc      540

cuccucaucg cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc      600

gagggcaugu ccggcggcac cugggucgac gucguccucg agcacggcgg cugcgucacc      660

gucauggccc aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac      720

auggccgagg uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc      780

cgcugcccca cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc      840

aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc      900

cucgucaccu gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc      960

gagaaccucg aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc     1020

gucaacgaca ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac     1080

uccccccgcg ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc     1140

cgcaccggcc ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc     1200

guccacaagg agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc     1320

cagaccgucg ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc     1380

cucgaggccg agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc     1440

cucaagaugg acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac     1560

gccggcaccg acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc     1620

acccccgucg gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc     1680

aagaugaugc ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc     1740

gagaagaaga ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc     1860

uccgucggcg gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaaguccc ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc     1980

auguggcucg gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc     2040

ggcguccuca ucuuccucuc caccgccguc uccgccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  3796
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 1 opt

<400>  3796
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc ccgccgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3797
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 1 opt

<400>  3797
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggaug uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3798
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 1 opt

<400>  3798
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaugaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3799
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 1 opt

<400>  3799
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgccaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

cucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucgcccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgccacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc gucgacggca ccgucaccgu cgagggccag     1560

uacggcggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau caucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3800
<211>  2275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 1 opt

<400>  3800
gggagaaagc uuaccauggg cgccgacgcc uccaucggca ucgucucccu ccuccucacc       60

accgucaugg ccgccgagau cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

uccgacgccg gcaaggccau cuccuucguc accacccucg gcgucaacaa gugccacguc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau caaggucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgucgccg ucgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucaccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga cgagaaccgc gccaaggucg aggucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacuccg gcgccgacac cgagaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

accaaggucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

cgcgacggcc ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgacaag     1740

aagaucaccc accacuggca ccgcuccggc uccaucaucg gcaaggccuu cgaggccacc     1800

guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc     1860

ggcggcgucu ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

ucccucuucg gcggcauguc cugguucucc cagauccuca ucggcacccu ccucgucugg     1980

cucggccuca acaccaagaa cggcuccauc ucccucaccu gccucgcccu cggcggcguc     2040

augaucuucc ucuccaccgc cgucuccgcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2220

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2275


<210>  3801
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 1 opt

<400>  3801
gggagaaagc uuaccauggg cgccgacacc uccaucggca ucaucggccu ccuccucacc       60

accgccaugg ccgccgagau cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

uccgacgccg gcaaggccau cuccuucgcc accaccaugg gcgucaacaa gugccacguc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc ccucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau caaggucgag aacuggaucc uccgcaaccc cggcuucacc      480

gucgccgccc ucgccauggc cuggcuccuc ggcaacucca ccucccagcg cgucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucaccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caucggccac gagaccgacg agaaccgcgc caaggucgag     1080

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac     1440

cucaagugcc gcaucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucuc caaggucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggccg cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccaucau caccgagucc     1680

accgagaacu cccgcaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgucuuc aacucccucg gcaagggcgu ccaccagguc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucgucuggcu cggccucaac accaagaacg gcuccaucuc ccucgccugc     2040

cucgcccucg gcggcgccau gaucuuccuc uccaccgccg ucuccgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3802
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 1 opt

<400>  3802
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3803
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 1 opt

<400>  3803
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3804
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 1 opt

<400>  3804
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc      540

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc      600

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      660

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      720

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc      840

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc      900

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc      960

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1020

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1140

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1200

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1260

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1320

auggccgucc ucggcgacac cgccugggac uucggcuccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3805
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 1 opt

<400>  3805
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3806
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 1 opt

<400>  3806
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3807
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 1 opt

<400>  3807
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaccgu caacgacauc      540

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3808
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 1 opt

<400>  3808
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc uccugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  3809
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 1 opt

<400>  3809
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc      540

gacgagaacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc      600

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      660

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      720

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc      840

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc      900

aagggcaagc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc      960

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1020

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1140

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1200

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1260

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1320

auggccgucc ucggcgacac cgccugggac uucggcuccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3810
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 1 opt

<400>  3810
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu cccuccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3811
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 1 opt

<400>  3811
gggagaaagc uuaccauguc caagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3812
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 1 opt

<400>  3812
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu gguaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3813
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 1 opt

<400>  3813
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggcaa gcucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3814
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 1 opt

<400>  3814
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaccc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3815
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 1 opt

<400>  3815
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagauc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3816
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 1 opt

<400>  3816
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgcccgcc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3817
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 1 opt

<400>  3817
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggucu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3818
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 1 opt

<400>  3818
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaucg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3819
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 1 opt

<400>  3819
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcaucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3820
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 1 opt

<400>  3820
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cucgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3821
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 1 opt

<400>  3821
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggca cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3822
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 1 opt

<400>  3822
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccucgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3823
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 1 opt

<400>  3823
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gaggcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3824
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 1 opt

<400>  3824
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccgccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3825
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 1 opt

<400>  3825
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc uccaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc caccaucucc gacaucgccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgccguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3826
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 1 opt

<400>  3826
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucuccaag     1020

auccccgccg agacccucca cggcaccguc accgaggagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3827
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 1 opt

<400>  3827
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagc uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3828
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 1 opt

<400>  3828
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcauc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcauccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3829
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 1 opt

<400>  3829
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ucaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3830
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 1 opt

<400>  3830
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcgccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3831
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 1 opt

<400>  3831
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac accggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gagggcaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3832
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 1 opt

<400>  3832
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgc cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugcucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgc ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg ccaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agaccgucga cggcaccguc accgucgagg gccaguacgg cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3833
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 1 opt

<400>  3833
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagcccg ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3834
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 1 opt

<400>  3834
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc aacaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3835
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 1 opt

<400>  3835
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcgccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3836
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 1 opt

<400>  3836
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3837
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 1 opt

<400>  3837
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu gguaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3838
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 1 opt

<400>  3838
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3839
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 1 opt

<400>  3839
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3840
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 1 opt

<400>  3840
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgucugcac cgccgccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3841
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 1 opt

<400>  3841
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acuccggcgc cgacaccgag accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggccgcg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ucaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc uccugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  3842
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 1 opt

<400>  3842
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugaucgu ccucaucguc       60

cccgccuacu ccaucuccug caucggcguc uccaaccgcg accucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccga gauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaugaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cucccucucc gacauggccu ccgccucccg cugccccacc      300

cagggcgagc ccucccucga caagcagucc gacacccagu ccgucugcaa gcgcacccuc      360

ggcgaccgcg gcuggggcaa cggcugcggc aucuucggca agggcucccu cgucaccugc      420

uccaaguuca ccugcugcaa gaagaugccc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcuccccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguccgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3843
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 1 opt

<400>  3843
gggagaaagc uuaccaugcg ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3844
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 1 opt

<400>  3844
gggagaaagc uuaccauguc ccagcgcguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgcau caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucuccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggccgcgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc caucaucacc gaguccaccg agaacucccg caugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3845
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  3845
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3846
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 opt

<400>  3846
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacacu      240

ugugacgcca ccaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac aaccaguacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacccuac cgucccauuc uacgaggaag      420

cuccaaacuc ggucgcagac cuggcuggag ucacgcgaau acacaaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacuuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcacag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccacc      840

caaggagaag ccuaccuuga caaacagagc gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugaccugc      960

gcaaaguuug cuugcucuaa gaaaaugacg gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cagacgagaa ucgagccaaa gucgaaauca cuccaaacuc accccgcgcu     1140

gaggccaccc uugggggauu cggcucccug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagug aucuguauua cuuaacuaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auaccggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau acugcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacgg ccgcauucac auuuaccaaa     1560

auaccugcgg aaacucucca uggcaccgug acaguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacucugac accugugggc     1680

agguuaauca cggccaaccc aguuauuacc gaaaguacug aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acacaccacu ggcaucguuc uggaucaacc aucgguaagg cuuuugaggc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acugccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaaugcaa agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa cggcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3847
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 opt

<400>  3847
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugg cuacgaaacc     1080

gaugaggacc gagcaaaggu cgaggugaca ccuaauucac cacgcgcuga agccacucuu     1140

gggggauucg gcagucuggg uuuggauugu gagcccagaa ccgggcucga cuuuagcgau     1200

cuguauuacu uaacaaugaa caauaaacac uggcugguac auaaggaaug guuccacgac     1260

auaccucucc cauggcacgc uggcgccgau acuggaaccc cgcauuggaa caauaaagag     1320

gcacuuguug aauuuaagga cgcccacgcu aagaggcaga caguggucgu gcugggcucc     1380

caggaggggg cgguccauac cgcauuggcc ggagcucuag aggccgaaau ggauggugca     1440

aaaggccggc uguucucugg gcaccucaag ugccgccuga aaauggacaa acuuagacug     1500

aagggaguga gcuauucauu guguacugcc gcuuuuacgu ucacaaaggu ucccgccgag     1560

acccuccaug gcacugugac cguagaaguc caguacgcag guacagacgg gccuugcaag     1620

aucccagugc aaauggcggu ugauaugcag acccugacuc ccgugggcag guuaauuaca     1680

gcuaacccug ucaucacgga gaguaccgaa aauuccaaaa ugaugcugga gcuagaccca     1740

cccuuuggag auagcuacau agugauuggc guaggggaca agaaaaucac ucaccacugg     1800

caucguucug gaucaacaau cgguaaggcc uucgaggcaa ccgugcgggg cgccaaacga     1860

auggcuguuc ucggggauac agccugggac uucggaagug ucggcggugu guuuaacagc     1920

cuggggaagg gcauucacca gaucuucgga gcagcuuuua agucccuuuu cggcgggaug     1980

ucuugguuuu cgcaaauuuu gauaggaacu cugcucgugu ggcugggccu uaacaccaaa     2040

aaugguagca ucucccugac guguuuggcc cucgggggag ucaugauuuu ccugucaacc     2100

gcgguuagug ccgauguggg cugcucugug gacuuuagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3848
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 1 opt

<400>  3848
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3849
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  3849
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3850
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 1 opt

<400>  3850
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cgccaagaau ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaacggca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3851
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 1 opt

<400>  3851
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgguuacg agaccgacga agaucgugcg aaggucgagg ugacaccuaa cucaccacgc     1080

gccgaggcaa cucuuggcgg guucggaagu cugggcuugg acugugaacc cagaaccggu     1140

cucgauuuua gcgaccugua uuacuuaaca augaauaaca aacacuggcu gguacauaag     1200

gagugguucc acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau     1260

uggaauaaca aagaagcucu uguugaguuu aaggaugccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucccagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggacg gagcuaaggg ucggcuguuc ucuggccacc ucaagugccg acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucauugugua cugccgcauu uacguucaca     1500

aagguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gacgguccuu gcaaaauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggagauuaa uaacagcaaa uccugucauc acggaaagua ccgagaacuc caagaugaug     1680

cuggaacuag auccacccuu uggcgacagc uacauuguga ucggaguagg cgauaagaaa     1740

auaacucacc acuggcauag gucuggguca acaauuggaa aggcguucga ggcuaccgug     1800

cggggugcca aacgcauggc aguucucggc gacacagccu gggauuucgg gagugucgga     1860

ggcguguuua auagccuggg uaaggggauc caccagaucu ucggcgcugc cuuuaaaucc     1920

cuuuucggag gcaugucuug guuuucgcaa auuuugaucg ggacucugcu cguguggcug     1980

ggacuuaaca ccaagaacgg cagcauuucc cugacguguu uggcacucgg uggggucaug     2040

auauuccugu caaccgcugu uagugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3852
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 1 opt

<400>  3852
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3853
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 opt

<400>  3853
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagauau cgugcuggaa cacggcgggu gugucacagu gauggcacaa      180

gacaaaccua cuguugauau ugaguuggug acgaccacag ucaguaauau ggccgaagug      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag guggaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ccauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ugaguucaag gaugcacacg ccaagagaca gacaguggua      840

guguugggcu cccaggaagg ggcuguccau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auccccgcug agacccugca cggcacuguu acaguggaag uccaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacguuaac cccgguuggc     1140

cgacugauaa cagcaaaucc cgugauuacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgucauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3854
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 opt

<400>  3854
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3855
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 opt

<400>  3855
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgg cuaugaaacc      540

gaugaggaca gggccaaggu ggaagucacg cccaacaguc cucgggcuga ggcaacucug      600

ggaggguuug gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau      660

cuguacuacc ucacaaugaa uaacaaacau ugguuaguuc acaaggaaug guuucaugac      720

auuccccugc cuuggcacgc cggagcugac acuggcaccc cacacuggaa uaacaaagag      780

gcccuugugg aguucaagga ugcgcaugca aagagacaga caguaguggu cuugggcucc      840

caggaagggg ccgugcacac cgcucuggcc ggagcacuag aagcugagau ggacggugcc      900

aaaggcaggc ucuuuucagg gcaucugaag ugucggcuua aaauggauaa gcugcgcuug      960

aaaggaguua gcuauagucu cugcacggcg gccuucacuu uuacaaaggu gcccgcagag     1020

acccugcacg gcacugucac aguggaagua caauacgcug guaccgacgg gccuuguaaa     1080

aucccaguuc agauggccgu ggauaugcag acguuaaccc cggucggccg acugauuaca     1140

gcuaaucccg ugaucacuga gucuaccgaa aacuccaaga ugaugcucga gcuugacccu     1200

ccauucggag auagcuauau agucauuggc gugggggaca agaaaaucac acaccauugg     1260

cacagaucgg gaucuacuau ugguaaggcc uuugaagcaa ccguuagggg cgccaaacgg     1320

auggcugugc ugggggauac agcgugggac uucggauccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  3856
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 1 opt

<400>  3856
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  3857
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       1 opt

<400>  3857
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcugag uggaaagggc      900

ucucuaguca caugcgcgaa auucgccugc uccaagaaaa ugaccggcaa gagcauucag      960

ccugaaaacc ucgaguaccg uaucaugcug ucggugcacg ggucucaaca cuccggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauucaccca gagcugaagc cacucuuggc ggguuuggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu cagcgaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ucacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggagu ucaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaucccag gaaggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gagacugaaa ggggugucau auaguuugug uacugccgcu     1500

uuuacguuca caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uuuggcgacu cuuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucaggga guacaauugg aaaggcauuc     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuu     1860

gggagcguag gaggcgcucu uaauucccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguucaaau cuuuguuugg aggcaugucg ugguucagcc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcuccauuu cauugaugug ucucgcccug     2040

gguggggugu uaauauuucu gaguacggcu guuucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3858
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 1
       opt

<400>  3858
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcugag uggaaagggc      900

ucucuaguga ccugcgcgaa auucgccugc uccaagaaaa ugacgggcaa gagcauacag      960

ccugaaaacc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauucaccca gagcugaagc cacccuuggc ggguuuggaa gucugggcuu ggacugugag     1140

ccuaggacag gucucgauuu cagcgaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ucacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagagu ucaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaucccag gaaggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gagacugaaa ggggucucau auaguuugug uacggccgcu     1500

uuuacauuca ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgaguc cacugaaaac     1680

agcaagauga ugcuggagcu agaucccccg uuuggcgacu cuuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucaggga guaccauagg aaaggcauuc     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuu     1860

gggagcgugg gaggcgcucu uaauucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguucaaau cuuuguuugg aggcaugucg ugguucagcc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cgccaagaau ggcuccaucu cauugaugug ucucgcucug     2040

ggugggguuu uaauuuuucu gaguacggca gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3859
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 1 
       opt

<400>  3859
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguc uggaaagggc      900

ucccuaguua caugcgccaa auuuaccugc agcaagaaga ugacgggcaa aucgauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucugugcacg ggucccaaca uucaggaaug     1020

aucgguuacg agaccgacga agaucgugcg aaggucgagg ugacaccuaa caguccacgc     1080

gccgaggcaa cucuuggcgg guucggaagc cugggcuugg acugugaacc cagaaccggu     1140

cucgauuucu ccgaccugua uuacuuaaca augaauaaca aacacuggcu gguacauaag     1200

gagugguuuc acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau     1260

uggaauaaca aagaagcucu uguugaguuc aaggaugccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucucagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggacg gagcuaaggg ucggcuguuu agcggccacc ucaagugccg acugaaaaug     1440

gauaaacuuc gccugaaggg ggugucauau aguuugugua cugccgcauu cacguuuaca     1500

aagguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gacgguccuu gcaaaauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggagauuaa uaacagcaaa uccugucauc acggaaucca ccgagaacag caagaugaug     1680

cuggaacuag auccacccuu cggcgacucu uacauuguga ucggaguagg cgauaagaaa     1740

auaacucacc acuggcauag gucagggagu acaauuggaa aggcguuuga ggcuaccgug     1800

cggggugcca aacgcauggc aguucucggc gacacagccu gggauuucgg gagcgucgga     1860

ggcguguuca auucccuggg uaaggggauc caccagaucu uuggcgcugc cuucaaaucu     1920

cuuuuuggag gcaugucgug guucagccaa auuuugaucg ggacucugcu cguguggcug     1980

ggacuuaaca ccaagaacgg cuccauuuca cugacguguu uggcacucgg uggggucaug     2040

auauuucuga guaccgcugu uucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3860
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 1 
       opt

<400>  3860
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcugag uggaaagggc      900

ucucuaguga caugcgcgaa auucgccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaacc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauucaccca gagcugaagc cacucuuggc ggguuuggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu cagcgaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ucacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagagu ucaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaucccag gaaggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gagacugaaa ggggucucau auaguuugug uacugccgcu     1500

uuuacguuca caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uuuggcgacu cuuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucaggga guacaauagg aaaggcauuc     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuu     1860

gggagcgugg gaggcgcucu uaauucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguucaaau cuuuguuugg aggcaugucg ugguucagcc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcuccaucu cauugaugug ucucgcccug     2040

ggugggguuu uaauuuuucu gaguacggcu gucucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3861
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       1 opt

<400>  3861
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguucagg acacggguca ugaaaccgau gagaaucgcg caaaggugga gaucacacca     1080

aacuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaauaa caaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucaa     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaga uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aauccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaacagccuu gguaagggga uccaccaaau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc agauucugau cgggacucuc     1980

cugauguggc uuggacugaa uaccaagaac ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3862
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 1
       opt

<400>  3862
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugcagg acaccgguca ugaaacagau gagaaucgcg caaaggucga gauuacucca     1080

aacuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcaa     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaga uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aauccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaacagccuu gguaagggga uucaccaaau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc agauccugau ugggacccuc     1980

cugauguggc uuggacugaa ugccaagaac ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaacggca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3863
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 1 
       opt

<400>  3863
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugcagg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcaa     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaga uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aauccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaacagccuu gguaagggga uucaccaaau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc agauccugau ugggacucuc     1980

cugauguggc uuggacugaa uaccaagaac ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3864
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  3864
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacacucgg aaaggcauuu     1800

aguaccacac ugaaaggugc ccagcgccuu gcugccuugg gcgauacugc augggacuuc     1860

gggagcauug gaggcgucuu caauuccauc gguagagcug ugcaccaagu auuugggggc     1920

gccuucagga cccuguuugg aggcaugucu uggaucacgc aggggcucau gggagcgcug     1980

cuucugugga ugggcgugaa cgcccgugau cggucgauug cuuuggcauu ccucgccacc     2040

ggugggguuc uggucuuuuu agcuacaaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3865
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  3865
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caacccucgg aaaggcauuu     1800

aguacaacuc ugaaaggugc ccagcgccuu gcugccuugg gcgauaccgc augggacuuc     1860

gggagcauag gaggcguguu caauuccauu gguagagcug uacaccaagu guuugggggc     1920

gccuucagga cgcuguuugg aggcaugucu uggaucaccc aggggcucau gggagcgcug     1980

cuucugugga ugggcguuaa cgcccgugau cggucgaucg cuuuggcauu ccucgccaca     2040

gguggggucc ugguguuuuu agcuacuaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3866
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 1 opt

<400>  3866
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgguuacg agaccgacga agaucgugcg aaggucgagg ugacaccuaa cucaccacgc     1080

gccgaggcaa cucuuggcgg guucggaagu cugggcuugg acugugaacc cagaaccggu     1140

cucgauuuua gcgaccugua uuacuuaaca augaauaaca aacacuggcu gguacauaag     1200

gagugguucc acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau     1260

uggaauaaca aagaagcucu uguugaguuu aaggaugccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucccagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggacg gagcuaaggg ucggcuguuc ucuggccacc ucaagugccg acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucauugugua cugccgcauu uacguucaca     1500

aagguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gacgguccuu gcaaaauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggagauuaa uaacagcaaa uccugucauc acggaaagua ccgagaacuc caagaugaug     1680

cuggaacuag auccacccuu uggcgacagc uacauuguga ucggaguagg cgauaagaaa     1740

auaacucacc acuggcauag gucuggguca acacucggaa aggcguucag uaccacacug     1800

aaaggugcuc agcggcuugc cgcauugggc gacacugccu gggauuucgg gagcauugga     1860

ggcguguuua auuccaucgg ucgcgcuguu caccaagucu ucgggggcgc cuuuagaacc     1920

cuguucggag gcaugucuug gaucacgcag gggcucaugg gagcacugcu ucuguggaug     1980

ggcgugaacg cuagggaccg uucgauugcc uuggcguuuc ucgccaccgg uggggugcug     2040

gucuucuuag cuacaaacgu ucaugcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  3867
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  3867
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacacucgg aaaggcauuu     1800

aguaccacac ugaaaggugc ccagcgccuu gcugccuugg gcgauacugc augggacuuc     1860

gggagcauag gaggcguguu caauuccauu gguagagcug uacaccaagu guuugggggc     1920

gccuucagga cccuguuugg aggcaugucu uggaucacgc aggggcucau gggagcgcug     1980

cuucugugga ugggcguuaa cgcccgugau cggucgaucg cuuuggcauu ccucgccacc     2040

gguggggucc ugguguuuuu agcuacaaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3868
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  3868
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3869
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3869
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagaggcg gggcgcugac       60

accagcgugg ggauagucgg acugcuccuu acaacugcaa uggccgcgga aguuacgcgc      120

cgagguuccg cuuacuauau guaccuggau cguaaugacg ccggcgaggc aaucucuuuc      180

cccaccacau uggggaugaa caaaugcuau auucagauca uggauuuagg acacaugugu      240

gacgccacua ugucauacga augcccuaug cuggaugagg gcguggaacc agacgaugua      300

gacuguuggu gcaauaccac aaguaccugg guggucuaug guacuuguca ucacaagaaa      360

ggggaggcua gaaggagccg gcgcgcagug acacuaccgu cccauucuac cagaaagcuc      420

caaacgaggu cgcagacuug gcuggaguca cgggaauaca ccaagcaccu uauucgcguu      480

gagaacugga ucuuucgaaa ucccggauuc gcccuggcug ccgcggcaau agccugguug      540

cucggcagca guacauccca gaaagugauc uaucugguca ugauucugcu caucgcuccu      600

gccuacucua uuagaugcau cggcgugagc aacagggauu uuguagaagg gauguccgga      660

gguacuuggg uugacauagu guuagagcau ggcgggugug ucaccgugau ggcacaggau      720

aagccaacag uggacauuga gcuggucacc acgacuguuu caaauauggc ugaagugcgg      780

aguuacugcu augaggccag caucucugau auggcguccg acucacguug ucccacacaa      840

ggagaagccu accuugacaa acagagcgau acccaguaug uaugcaagcg cacuuuggug      900

gacagaggcu gggguaacgg guguggacug uucggcaaag gcagucuagu cacaugcgca      960

aaguuugcuu gcucuaagaa aaugaccggg aaguccauuc agccugagaa ucucgaauac     1020

aggaucaugc ugagcgugca cggaucgcaa cacucuggua ugaucguuaa cgauacgggc     1080

caugagaccg acgagaaucg ggccaaagug gaaauuacac caaacucacc ccgagcugag     1140

gccacucuug ggggauucgg cucccugggu uuggauugug aaccucgcac cgggcucgac     1200

uuuagugauc uguauuacuu aacaaugaau aacaagcacu ggcuggucca uaaagagugg     1260

uuccacgaca uaccacuccc guggcacgca ggcgccgaua cuggaacacc ccauuggaau     1320

aacaaggaag cucuugugga guuuaaagac gcgcacgcaa agagacagac cguaguugug     1380

cugggcagcc aggagggggc cguccauacc gcuuuggccg gagcacuaga agccgagaug     1440

gauggugcua agggcaggcu guccucuggg caccucaaau gccggcugaa gauggacaaa     1500

cuucgccuga agggagugag cuauucauug uguacugccg cauucacguu uacaaaaauc     1560

ccugcggaaa cccuccaugg cacugucacc guggagguuc aguacgcugg uacagacggg     1620

ccaugcaagg ugcccgccca aauggcagua gauaugcaga cccugacucc ugucggcaga     1680

uuaauuacag ccaacccagu gaucacggaa aguaccgaaa auuccaagau gaugcuggag     1740

cuagaucccc cguucggaga cagcuacaua guuauuggcg ugggggagaa aaagaucacu     1800

caccacuggc auaggucugg aucaacaauc gguaaggcuu uugaggccac cguccguggc     1860

gcaaaacgga uggcugugcu cggggacaca gccugggauu ucggaagugu aggcggugcg     1920

cugaacagcc uugggaaagg cauucaccag aucuucggag ccgcuuuuaa guccuuguuc     1980

ggcgggaugu cuugguuuuc gcaaauucug auaggaacuc uccugaugug gcuuggccug     2040

aauaccaaga acgguagcau cuccuugaug ugucucgcau uagggggagu gcugauuuuc     2100

cugucaacgg ccguuagugc ugacgucggc ugcucugugg auuuuagcaa augaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  3870
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3870
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agaggcgggg cgcugacacc       60

agcgugggga uugucggacu gcuccuuaca acugcaaugg ccgcggaagu uacgcgccga      120

gguuccgcuu acuauaugua ccuggaucgu aaugacgccg gcgaggcaau aucuuucccc      180

accacauugg ggaugaacaa augcuauauc cagauuaugg auuuaggaca caugugugac      240

gccacuaugu cauacgaaug cccuaugcug gaugagggcg uggaaccaga cgauguagac      300

uguuggugca auaccacaag uaccugggug gucuauggua cuugucauca caagaaaggg      360

gaggcuagaa ggagccggcg cgcagugaca cuaccguccc auucuaccag aaagcuccaa      420

acgaggucgc agacuuggcu ggagucacgg gaauacacca agcaccuuau ccgcguugag      480

aacuggauuu uucgaaaucc cggauucgcc cuggcugccg cggcaaucgc cugguugcuc      540

ggcagcagua caucccagaa agugauauau cuggucauga uccugcucau ugcuccugcc      600

uacucuauca gaugcauugg cgugagcaac agggauuuug uagaagggau guccggaggu      660

acuuggguug acaucguguu agagcauggc ggguguguca ccgugauggc acaggauaag      720

ccaacagugg acauagagcu ggucaccacg acuguuucaa auauggcuga agugcggagu      780

uacugcuaug aggccagcau uucugauaug gcguccgacu cacguugucc cacacaagga      840

gaagccuacc uugacaaaca gagcgauacc caguauguau gcaagcgcac uuugguggac      900

agaggcuggg guaacgggug uggacuguuc ggcaaaggca gucuagucac augcgcaaag      960

uuugcuugcu cuaagaaaau gaccgggaag uccauccagc cugagaaucu cgaauacagg     1020

auuaugcuga gcgugcacgg aucgcaacac ucugguauga ucguuaacga uacgggccau     1080

gagaccgacg agaaucgggc caaaguggaa aucacaccaa acucaccccg agcugaggcc     1140

acucuugggg gauucggcuc ccuggguuug gauugugaac cucgcaccgg gcucgacuuu     1200

agugaucugu auuacuuaac aaugaauaac aagcacuggc ugguccauaa agagugguuc     1260

cacgacauuc cacucccgug gcacgcaggc gccgauacug gaacacccca uuggaauaac     1320

aaggaagcuc uuguggaguu uaaagacgcg cacgcaaaga gacagaccgu aguugugcug     1380

ggcagccagg agggggccgu ccauaccgcu uuggccggag cacuagaagc cgagauggau     1440

ggugcuaagg gcaggcuguc cucugggcac cucaaaugcc ggcugaagau ggacaaacuu     1500

cgccugaagg gagugagcua uucauugugu acugccgcau ucacguuuac aaaaauaccu     1560

gcggaaaccc uccauggcac ugucaccgug gagguucagu acgcugguac agacgggcca     1620

ugcaaggugc ccgcccaaau ggcaguagau augcagaccc ugacuccugu cggcagauua     1680

aucacagcca acccagugau uacggaaagu accgaaaauu ccaagaugau gcuggagcua     1740

gaucccccgu ucggagacag cuacaucguu auaggcgugg gggagaaaaa gauuacucac     1800

cacuggcaua ggucuggauc aacaaucggu aaggcuuuug aggccaccgu ccguggcgca     1860

aaacggaugg cugugcucgg ggacacagcc ugggauuucg gaaguguagg cggugcgcug     1920

aacagccuug ggaaaggcau ccaccagauu uucggagccg cuuuuaaguc cuuguucggc     1980

gggaugucuu gguuuucgca aauccugauu ggaacucucc ugauguggcu uggccugaau     2040

accaagaacg guagcauauc cuugaugugu cucgcauuag ggggagugcu gaucuuccug     2100

ucaacggccg uuagugcuga cgucggcugc ucuguggauu uuagcaaaug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  3871
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3871
gggagaaagc uuaccaugaa cgccagaaag gagaaaaaga ggcggggcgc ugacaccagc       60

guggggaucg ucggacugcu ccuuacaacu gcaauggccg cggaaguuac gcgccgaggu      120

uccgcuuacu auauguaccu ggaucguaau gacgccggcg aggcaauuuc uuuccccacc      180

acauugggga ugaacaaaug cuauauacag aucauggauu uaggacacau gugugacgcc      240

acuaugucau acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaua ccacaaguac cugggugguc uaugguacuu gucaucacaa gaaaggggag      360

gcuagaagga gccggcgcgc agugacacua ccgucccauu cuaccagaaa gcuccaaacg      420

aggucgcaga cuuggcugga gucacgggaa uacaccaagc accuuauucg cguugagaac      480

uggaucuuuc gaaaucccgg auucgcccug gcugccgcgg caauugccug guugcucggc      540

agcaguacau cccagaaagu gaucuaucug gucaugauac ugcucaucgc uccugccuac      600

ucuauuagau gcaucggcgu gagcaacagg gauuuuguag aagggauguc cggagguacu      660

uggguugaca uuguguuaga gcauggcggg ugugucaccg ugauggcaca ggauaagcca      720

acaguggaca ucgagcuggu caccacgacu guuucaaaua uggcugaagu gcggaguuac      780

ugcuaugagg ccagcauauc ugauauggcg uccgacucac guugucccac acaaggagaa      840

gccuaccuug acaaacagag cgauacccag uauguaugca agcgcacuuu gguggacaga      900

ggcuggggua acgggugugg acuguucggc aaaggcaguc uagucacaug cgcaaaguuu      960

gcuugcucua agaaaaugac cgggaagucc auucagccug agaaucucga auacaggauc     1020

augcugagcg ugcacggauc gcaacacucu gguaugauug uuaacgauac gggccaugag     1080

accgacgaga aucgggccaa aguggaaauc acaccaaacu caccccgagc ugaggccacu     1140

cuugggggau ucggcucccu ggguuuggau ugugaaccuc gcaccgggcu cgacuuuagu     1200

gaucuguauu acuuaacaau gaauaacaag cacuggcugg uccauaaaga gugguuccac     1260

gacaucccac ucccguggca cgcaggcgcc gauacuggaa caccccauug gaauaacaag     1320

gaagcucuug uggaguuuaa agacgcgcac gcaaagagac agaccguagu ugugcugggc     1380

agccaggagg gggccgucca uaccgcuuug gccggagcac uagaagccga gauggauggu     1440

gcuaagggca ggcuguccuc ugggcaccuc aaaugccggc ugaagaugga caaacuucgc     1500

cugaagggag ugagcuauuc auuguguacu gccgcauuca cguuuacaaa aauuccugcg     1560

gaaacccucc auggcacugu caccguggag guucaguacg cugguacaga cgggccaugc     1620

aaggugcccg cccaaauggc aguagauaug cagacccuga cuccugucgg cagauuaaua     1680

acagccaacc cagugaucac ggaaaguacc gaaaauucca agaugaugcu ggagcuagau     1740

cccccguucg gagacagcua cauuguuauc ggcguggggg agaaaaagau aacucaccac     1800

uggcauaggu cuggaucaac aauugguaag gcuuuugagg ccaccguccg uggcgcaaaa     1860

cggauggcug ugcucgggga cacagccugg gauuucggaa guguaggcgg ugcgcugaac     1920

agccuuggga aaggcaucca ccagaucuuc ggagccgcuu uuaaguccuu guucggcggg     1980

augucuuggu uuucgcaaau ucugaucgga acucuccuga uguggcuugg ccugaauacc     2040

aagaacggua gcauuuccuu gaugugucuc gcauuagggg gagugcugau auuccuguca     2100

acggccguua gugcugacgu cggcugcucu guggauuuua gcaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3872
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3872
gggagaaagc uuaccauggc cagaaaggag aaaaagaggc ggggcgcuga caccagcgug       60

gggaucgucg gacugcuccu uacaacugca auggccgcgg aaguuacgcg ccgagguucc      120

gcuuacuaua uguaccugga ucguaacgac gccggcgagg caauuucuuu ccccaccaca      180

uuggggauga auaaaugcua uauacagauc auggauuuag gacacaugug ugacgccacu      240

augucauacg aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg      300

ugcaacacca caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu      360

agaaggagcc ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccaaacgagg      420

ucgcagacuu ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaauugg      480

aucuuucgaa accccggauu cgcccuggcu gccgcggcaa uugccugguu gcucggcagc      540

aguacauccc agaaagugau cuaucugguc augauacugc ucaucgcucc ugccuacucu      600

auuagaugca ucggcgugag caauagggau uuuguagaag ggauguccgg agguacuugg      660

guugacauug uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca      720

guggacaucg agcuggucac cacgacuguu ucaaacaugg cugaagugcg gaguuacugc      780

uaugaggcca gcauaucuga uauggcgucc gacucacguu gucccacaca aggagaagcc      840

uaccuugaca aacagagcga uacccaguau guaugcaagc gcacuuuggu ggacagaggc      900

ugggguaaug gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu      960

ugcucuaaga aaaugaccgg gaaguccauu cagccugaga accucgaaua caggaucaug     1020

cugagcgugc acggaucgca acacucuggu augauuguua augauacggg ccaugagacc     1080

gacgagaacc gggccaaagu ggaaaucaca ccaaauucac cccgagcuga ggccacucuu     1140

gggggauucg gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau     1200

cuguauuacu uaacaaugaa caauaagcac uggcuggucc auaaagagug guuccacgac     1260

aucccacucc cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa     1320

gcucuugugg aguuuaaaga cgcgcacgca aagagacaga ccguaguugu gcugggcagc     1380

caggaggggg ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu     1440

aagggcaggc uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcauucacgu uuacaaaaau uccugcggaa     1560

acccuccaug gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag     1620

gugcccgccc aaauggcagu agauaugcag acccugacuc cugucggcag auuaauaaca     1680

gccaacccag ugaucacgga aaguaccgag aacuccaaga ugaugcugga gcuagauccc     1740

ccguucggag acagcuacau uguuaucggc gugggggaga aaaagauaac ucaccacugg     1800

cauaggucug gaucaacaau ugguaaggcu uuugaagcca ccguccgugg cgcaaaacgg     1860

auggcugugc ucggggacac agccugggau uucggaagug uaggcggugc gcugaauagc     1920

cuugggaaag gcauccacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug     1980

ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag     2040

aaugguagca uuuccuugau gugucucgca uuagggggag ugcugauauu ccugucaacg     2100

gccguuagug cugacgucgg cugcucugug gauuuuagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3873
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3873
gggagaaagc uuaccaugag aaaggagaaa aagaggcggg gcgccgacac cagcgugggg       60

aucgucggac ugcuccuuac aacugcuaug gcagccgaag uuacgcgccg agguuccgcg      120

uacuauaugu accuggaucg uaacgacgcu ggcgaggcca uuucuuuccc caccacauug      180

gggaugaaua aaugcuauau acagaucaug gauuuaggac acauguguga cgcaacuaug      240

ucauacgaau gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc      300

aacaccacaa guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggccaga      360

aggagccggc gcgcugugac acuaccgucc cauucuacca gaaagcucca aacgaggucg      420

cagacuuggc uggagucacg ggaauacacc aagcaccuua uucgcguuga gaauuggauc      480

uuucgaaacc ccggauucgc acuggccgcu gccgcgauug caugguugcu cggcagcagu      540

acaucccaga aagugaucua ucuggucaug auacugcuca ucgccccugc uuacucuauu      600

agaugcaucg gcgugagcaa uagggauuuu guagaaggga uguccggagg uacuuggguu      660

gacauugugu uagagcaugg cggguguguc accgugaugg cccaggauaa gccaacagug      720

gacaucgagc uggucaccac gacuguuuca aacauggcag aagugcggag uuacugcuau      780

gaggcuagca uaucugauau ggccuccgac ucacguuguc ccacacaagg agaagcguac      840

cuugacaaac agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg      900

gguaaugggu guggacuguu cggcaaaggc agucuaguca caugcgccaa guuugcaugc      960

ucuaagaaaa ugaccgggaa guccauucag ccugagaacc ucgaauacag gaucaugcug     1020

agcgugcacg gaucgcaaca cucugguaug auuguuaaug auacgggcca ugagaccgac     1080

gagaaccggg cuaaagugga aaucacacca aauucacccc gagccgaggc uacucuuggg     1140

ggauucggcu cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug     1200

uauuacuuaa caaugaacaa uaagcacugg cugguccaua aagagugguu ccacgacauc     1260

ccacucccgu ggcacgccgg cgcagauacu ggaacacccc auuggaacaa uaaggaagcc     1320

cuuguggagu uuaaagacgc ucacgcgaag agacagaccg uaguugugcu gggcagccag     1380

gagggggcag uccauaccgc cuuggcugga gcccuagaag cagagaugga uggugccaag     1440

ggcaggcugu ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag     1500

ggagugagcu auucauugug uacugcugcc uucacguuua caaaaauucc ugcagaaacc     1560

cuccauggca cugucaccgu ggagguucag uacgcgggua cagacgggcc augcaaggug     1620

cccgcucaaa uggccguaga uaugcagacc cugacuccug ucggcagauu aauaacagca     1680

aacccaguga ucacggaaag uaccgagaac uccaagauga ugcuggagcu agaucccccg     1740

uucggagaca gcuacauugu uaucggcgug ggggagaaaa agauaacuca ccacuggcau     1800

aggucuggau caacaauugg uaaagccuuu gaagcuaccg uccguggcgc caagcggaug     1860

gcagugcucg gggacacagc uugggauuuc ggaaguguag gcggugcccu gaauagccuu     1920

gggaaaggca uccaccagau cuucggagcg gccuuuaagu ccuuguucgg cgggaugucu     1980

ugguuuucgc aaauucugau cggaacucuc cugauguggc uuggccugaa caccaagaau     2040

gguagcauuu ccuugaugug ucucgcuuua gggggagugc ugauauuccu gucaacggca     2100

guuagugccg acgucggcug cucuguggau uuuagcaaau gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  3874
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3874
gggagaaagc uuaccaugaa ggagaaaaag agaaggggcg ccgacaccag cguggggauc       60

gucggacugc uccuuacaac ugcuauggca gccgaaguua cgcggcgcgg uuccgcguac      120

uauauguacc uggaucgaaa cgacgcuggc gaggccauuu cuuuccccac cacauugggg      180

augaauaaau gcuauauaca gaucauggau uuaggacaca ugugugacgc aacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcccguaga      360

agcaggcggg cugugacacu accgucccau ucuacccgca agcuccaaac gagaucgcag      420

acuuggcugg agucaaggga auacaccaag caccuuauuc ggguugagaa uuggaucuuu      480

cgcaaccccg gauucgcacu ggccgcugcc gcgauugcau gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugaua cugcucaucg ccccugcuua cucuauucga      600

ugcaucggcg ugagcaauag agauuuugua gaagggaugu ccggagguac uuggguugac      660

auuguguuag agcauggcgg gugugucacc gugauggccc aggauaagcc aacaguggac      720

aucgagcugg ucaccacgac uguuucaaac auggcagaag ugaggaguua cugcuaugag      780

gcuagcauau cugauauggc cuccgacuca cgguguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guauguaugc aagcguacuu ugguggaccg cggcuggggu      900

aaugggugug gacuguucgg caaaggcagu cuagucacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaccucg aauacagaau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauu guuaaugaua cgggccauga gaccgacgag     1080

aacagggcua aaguggaaau cacaccaaau ucaccccggg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagcgc cagaccguag uugugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

agacuguccu cugggcaccu caaaugcagg cugaagaugg acaaacuucg gcugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauuccugc agaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gccgcuuaau aacagcaaac     1680

ccagugauca cggaaaguac cgagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauuguuau cggcgugggg gagaaaaaga uaacucacca cuggcauaga     1800

ucuggaucaa caauugguaa agccuuugaa gcuaccguca ggggcgccaa gcguauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

agcauuuccu ugaugugucu cgcuuuaggg ggagugcuga uauuccuguc aacggcaguu     2100

agugccgacg ucggcugcuc uguggauuuu agcaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3875
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3875
gggagaaagc uuaccaugga gaagaaaaga aggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc uuacaacugc uauggcagcc gaaguuacgc ggcgcgguuc cgcguacuau      120

auguaccugg aucgaaacga cgcuggcgag gccauuucuu uccccaccac auuggggaug      180

aauaagugcu auauacagau cauggauuua ggacacaugu gugacgcaac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaaa agggggaggc ccguagaagc      360

aggcgggcug ugacacuacc gucccauucu acccgcaaac uccaaacgag aucgcagacu      420

uggcuggagu caagggaaua caccaagcac cuuauucggg uugagaauug gaucuuucgc      480

aaccccggau ucgcacuggc cgcugccgcg auugcauggu ugcucggcag caguacaucc      540

cagaagguga ucuaucuggu caugauacug cucaucgccc cugcuuacuc uauucgaugc      600

aucggcguga gcaauagaga uuuuguagaa gggauguccg gagguacuug gguugacauu      660

guguuagagc auggcgggug ugucaccgug auggcccagg auaaaccaac aguggacauc      720

gagcugguca ccacgacugu uucaaacaug gcagaaguga ggaguuacug cuaugaggcu      780

agcauaucug auauggccuc cgacucacgg ugucccacac aaggagaagc guaccuugac      840

aagcagagcg auacccagua uguaugcaaa cguacuuugg uggaccgcgg cugggguaau      900

ggguguggac uguucggcaa gggcagucua gucacaugcg ccaaauuugc augcucuaag      960

aagaugaccg ggaaauccau ucagccugag aaccucgaau acagaaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugauuguu aaugauacgg gccaugagac cgacgagaac     1080

agggcuaagg uggaaaucac accaaauuca ccccgggccg aggcuacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucga accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga acaauaaaca cuggcugguc cauaaggagu gguuccacga caucccacuc     1260

ccguggcacg ccggcgcaga uacuggaaca ccccauugga acaauaaaga agcccuugug     1320

gaguuuaagg acgcucacgc gaaacgccag accguaguug ugcugggcag ccaggagggg     1380

gcaguccaua ccgccuuggc uggagcccua gaagcagaga uggauggugc caagggcaga     1440

cuguccucug ggcaccucaa gugcaggcug aaaauggaca agcugcggcu gaaaggagug     1500

agcuauucau uguguacugc ugccuucacg uuuacaaaga uuccugcaga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcg gguacagacg ggccaugcaa agugcccgcu     1620

caaauggccg uagauaugca gacccugacu ccugucggcc gcuuaauaac agcaaaccca     1680

gugaucacgg aaaguaccga gaacuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uuguuaucgg cgugggggaa aagaaaauaa cucaccacug gcauagaucu     1800

ggaucaacaa uugguaaggc cuuugaggcu accgucaggg gcgccaaacg uauggcagug     1860

cucggggaca cagcuuggga uuucggaagu guaggcggug cccugaauag ccuugggaag     1920

ggcauccacc agaucuucgg agcggccuuu aaauccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugaucggaac ucuccugaug uggcuuggcc ugaacaccaa gaaugguagc     2040

auuuccuuga ugugucucgc ucuuggggga guguuaauau uccugucaac ggcaguuagu     2100

gccgacgucg gcugcucugu ggauuuuagc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  3876
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3876
gggagaaagc uuaccaugaa gaaaagaagg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuua caacugcuau ggcagccgag guuacgcggc gcgguuccgc guacuauaug      120

uaccuggauc gaaacgacgc uggcgaagcc auuucuuucc ccaccacauu ggggaugaau      180

aagugcuaua uacagaucau ggauuuagga cacaugugug acgcaacuau gucauacgag      240

ugcccuaugc uggaugaagg cguggagcca gacgauguag acuguuggug caacaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaaaaagg gggaagcccg uagaagcagg      360

cgggcuguga cacuaccguc ccauucuacc cgcaaacucc aaacgagauc gcagacuugg      420

cuggagucaa gggaguacac caagcaccuu auucggguug aaaauuggau cuuucgcaac      480

cccggauucg cacuggccgc ugccgcgauu gcaugguugc ucggcagcag uacaucccag      540

aaggugaucu aucuggucau gauacugcuc aucgccccug cuuacucuau ucgaugcauc      600

ggcgugagca auagagauuu uguagagggg auguccggag guacuugggu ugacauugug      660

uuagaacaug gcgggugugu caccgugaug gcccaggaua aaccaacagu ggacaucgag      720

cuggucacca cgacuguuuc aaacauggca gaggugagga guuacugcua ugaagcuagc      780

auaucugaua uggccuccga cucacggugu cccacacaag gagaggcgua ccuugacaag      840

cagagcgaua cccaguaugu augcaaacgu acuuuggugg accgcggcug ggguaauggg      900

uguggacugu ucggcaaggg cagucuaguc acaugcgcca aauuugcaug cucuaagaag      960

augaccggga aauccauuca gccugaaaac cucgaguaca gaaucaugcu gagcgugcac     1020

ggaucgcaac acucugguau gauuguuaau gauacgggcc augaaaccga cgagaacagg     1080

gcuaaggugg agaucacacc aaauucaccc cgggccgaag cuacucuugg gggauucggc     1140

ucccuggguu uggauuguga gccucgaacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgacau cccacucccg     1260

uggcacgccg gcgcagauac uggaacaccc cauuggaaca auaaagaggc ccuuguggaa     1320

uuuaaggacg cucacgcgaa acgccagacc guaguugugc ugggcagcca ggagggggca     1380

guccauaccg ccuuggcugg agcccuagag gcagaaaugg auggugccaa gggcagacug     1440

uccucugggc accucaagug caggcugaaa auggacaagc ugcggcugaa aggagugagc     1500

uauucauugu guacugcugc cuucacguuu acaaagauuc cugcagagac ccuccauggc     1560

acugucaccg uggaaguuca guacgcgggu acagacgggc caugcaaagu gcccgcucaa     1620

auggccguag auaugcagac ccugacuccu gucggccgcu uaauaacagc aaacccagug     1680

aucacggaga guaccgaaaa cuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacauug uuaucggcgu gggggagaag aaaauaacuc accacuggca uagaucugga     1800

ucaacaauug guaaggccuu ugaagcuacc gucaggggcg ccaaacguau ggcagugcuc     1860

ggggacacag cuugggauuu cggaagugua ggcggugccc ugaauagccu ugggaagggc     1920

auccaccaga ucuucggagc ggccuuuaaa uccuuguucg gcgggauguc uugguuuucg     1980

caaauucuga ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu     2040

uccuugaugu gucucgcucu ugggggagug uuaauauucc ugucaacggc aguuagugcc     2100

gacgucggcu gcucugugga uuuuagcaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  3877
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3877
gggagaaagc uuaccaugaa gagaaggggc gccgacacca gcguggggau cgucggacug       60

cuccuuacaa cugcuauggc agccgagguu acgcggcgcg guuccgcgua cuauauguac      120

cuggaucgaa acgacgcugg cgaagccauu ucuuucccca ccacauuggg gaugaauaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg caacuauguc auacgagugc      240

ccuaugcugg augaaggcgu ggagccagac gauguagacu guuggugcaa caccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aagcccguag aagcaggcgg      360

gcugugacac uaccguccca uucuacccgc aagcuccaaa cgagaucgca gacuuggcug      420

gagucaaggg aguacaccaa acaccuuauu cggguugaaa auuggaucuu ucgcaacccc      480

ggauucgcac uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaag      540

gugaucuauc uggucaugau acugcucauc gccccugcuu acucuauucg augcaucggc      600

gugagcaaua gagauuuugu agaggggaug uccggaggua cuuggguuga cauuguguua      660

gaacauggcg ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa cauggcagag gugaggaguu acugcuauga agcuagcaua      780

ucugauaugg ccuccgacuc acgguguccc acacaaggag aggcguaccu ugacaaacag      840

agcgauaccc aguauguaug caagcguacu uugguggacc gcggcugggg uaaugggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaaaaagaug      960

accgggaagu ccauucagcc ugaaaaccuc gaguacagaa ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaaugau acgggccaug aaaccgacga gaacagggcu     1080

aaaguggaga ucacaccaaa uucaccccgg gccgaagcua cucuuggggg auucggcucc     1140

cuggguuugg auugugagcc ucgaaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaacaaua agcacuggcu gguccauaaa gaaugguucc acgacauccc acucccgugg     1260

cacgccggcg cagauacugg aacaccccau uggaacaaua aggaggcccu uguggaauuu     1320

aaagacgcuc acgcgaagcg ccagaccgua guugugcugg gcagccagga gggggcaguc     1380

cauaccgccu uggcuggagc ccuagaggca gaaauggaug gugccaaagg cagacugucc     1440

ucugggcacc ucaagugcag gcugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucauugugua cugcugccuu cacguuuaca aaaauuccug cagagacccu ccauggcacu     1560

gucaccgugg aaguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug     1620

gccguagaua ugcagacccu gacuccuguc ggccgcuuaa uaacagcaaa cccagugauc     1680

acggagagua ccgaaaacuc caaaaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaagaag auaacucacc acuggcauag aucuggauca     1800

acaauuggua aagccuuuga agcuaccguc aggggcgcca agcguauggc agugcucggg     1860

gacacagcuu gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc     1920

caccagaucu ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaca ccaaaaaugg uagcauuucc     2040

uugauguguc ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac     2100

gucggcugcu cuguggauuu uagcaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3878
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3878
gggagaaagc uuaccaugag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug cuauggcagc cgagguuacg cggcgcgguu ccgcguacua uauguaccug      120

gaucgaaacg acgcuggcga agccauuucu uuccccacca cauuggggau gaauaagugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgagugcccu      240

augcuggaug aaggcgugga gccagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaaa aagggggaag cccguagaag caggcgggcu      360

gugacacuac cgucccauuc uacccgcaaa cuccaaacga gaucgcagac uuggcuggag      420

ucaagggagu acaccaagca ccuuauucgg guugaaaauu ggaucuuucg caaccccgga      480

uucgcacugg ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug      600

agcaauagag auuuuguaga ggggaugucc ggagguacuu ggguugacau uguguuagaa      660

cauggcgggu gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcagaggug aggaguuacu gcuaugaagc uagcauaucu      780

gauauggccu ccgacucacg gugucccaca caaggagagg cguaccuuga caagcagagc      840

gauacccagu auguaugcaa acguacuuug guggaccgcg gcugggguaa ugggugugga      900

cuguucggca agggcagucu agucacaugc gccaaauuug caugcucuaa gaaaaugacc      960

gggaagucca uucagccuga aaaccucgag uacagaauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaaa ccgacgagaa cagggcuaag     1080

guggagauca caccaaauuc accccgggcc gaagcuacuc uugggggauu cggcucccug     1140

gguuuggauu gugagccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu ccauaaggaa ugguuccacg acaucccacu cccguggcac     1260

gccggcgcag auacuggaac accccauugg aacaauaaag aggcccuugu ggaauuuaag     1320

gacgcucacg cgaaacgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau     1380

accgccuugg cuggagcccu agaggcagaa auggauggug ccaagggcag acuguccucu     1440

gggcaccuca aaugcaggcu gaagauggac aagcugcggc ugaaaggagu gagcuauuca     1500

uuguguacug cugccuucac guuuacaaag auuccugcag agacccucca uggcacuguc     1560

accguggaag uucaguacgc ggguacagac gggccaugca aagugcccgc ucaaauggcc     1620

guagauaugc agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca     1800

auugguaagg ccuuugaagc uaccgucagg ggcgccaaac guauggcagu gcucggggac     1860

acagcuuggg auuucggaag uguaggcggu gcccugaaua gccuugggaa gggcauccac     1920

cagaucuucg gagcggccuu uaaauccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cucuuggggg aguguuaaua uuccugucaa cggcaguuag ugccgacguc     2100

ggcugcucug uggauuuuag caaaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3879
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3879
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga gguuacgagg cgggguuccg cguacuauau guaccuggau      120

cgcaacgacg cuggcgaagc cauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga gugcccuaug      240

cuggaugaag gcguggagcc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaagccc gacguagcag aagggcugug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaguca      420

agagaguaca ccaagcaccu uauuaggguu gaaaauugga ucuuucggaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaggg gauguccgga gguacuuggg uugacauugu guuagaacau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaggugaga aguuacugcu augaagcuag cauaucugau      780

auggccuccg acucaaggug ucccacacaa ggagaggcgu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugaaaa ccucgaguac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugaaaccg acgagaacag agcuaaggug     1080

gagaucacac caaauucccc cagggccgaa gcuacucuug ggggauucgg cucacugggu     1140

uuggauugug agccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggaaugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaagagg cccuugugga auuuaaggac     1320

gcucacgcga aacgacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga ggcagaaaug gauggugcca agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaagauu ccugcagaga cccuccaugg cacugucacc     1560

guggaaguuc aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag caaacccagu gaucacggag     1680

aguaccgaaa acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggccu uugaagcuac cgucagaggc gccaaaagga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuc uugggggagu guuaauauuc cugucaacgg caguuagugc cgacgucggc     2100

ugcucugugg auuuuagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3880
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3880
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaggu uacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

aacgacgcug gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug      240

gaugaaggcg uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caaaaagggg gaagcccgcc gaagccguag agcugugaca      360

cuaccguccc auucuaccag gaaacuccaa acgcggucgc agacuuggcu ggagucacgc      420

gaguacacca agcaccuuau uagaguugaa aauuggaucu uuaggaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc ggugcaucgg cgugagcaau      600

cgcgauuuug uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga ggugcgaagu uacugcuaug aagcuagcau aucugauaug      780

gccuccgacu caagaugucc cacacaagga gaggcguacc uugacaagca gagcgauacc      840

caguauguau gcaaaaggac uuugguggac cggggcuggg guaaugggug uggacuguuc      900

ggcaagggca gucuagucac augcgccaaa uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugaaaaccu cgaguaccgu aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gaaaccgacg agaaccgcgc uaagguggag     1080

aucacaccaa auucccccag agccgaagcu acucuugggg gauucggcuc acuggguuug     1140

gauugugagc cuaggaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aaacacuggc ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu     1320

cacgcgaaac ggcagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaggc agaaauggau ggugccaagg gccgacuguc cucugggcac     1440

cucaaaugcc gccugaagau ggacaagcug agacugaaag gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug     1560

gaaguucagu acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggcagguua auaacagcaa acccagugau cacggagagu     1680

accgaaaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcauc ggucuggauc aacaauuggu     1800

aaggccuuug aagcuaccgu ccgcggcgcc aaaagaaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc     1920

uucggagcgg ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcucuug ggggaguguu aauauuccug ucaacggcag uuagugccga cgucggcugc     2100

ucuguggauu uuagcaaaug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3881
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3881
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccuuacaacu       60

gcuauggcag ccgagguuac gagaagggga uccgcguacu auauguaccu ggaucggaac      120

gacgcuggug aagccauuuc uuuccccacc acauugggca ugaauaagug cuauauacag      180

aucauggauu uagggcacau gugugacgca acuaugucau acgagugccc uaugcuggau      240

gaaggagugg agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uauggcacuu gucaucacaa aaagggugaa gcccgccgaa gccguagagc ugugacacua      360

ccgucccauu cuaccaggaa acuccaaacg cggucgcaga cuuggcugga gucacgcgag      420

uacaccaagc accuuauuag aguugaaaau uggaucuuua ggaaccccgg guucgcacug      480

gccgcugccg cgauugcaug guugcucgga agcaguacau cccagaaagu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucggu gcaucggcgu gagcaaucgc      600

gauuuuguag agggcauguc cgggggaacu uggguugaca uuguguuaga acaugguggc      660

ugugucaccg ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaggu gcgaaguuac ugcuaugaag cuagcauauc ugauauggcc      780

uccgacucaa gaugucccac acaaggggag gcguaccuug acaagcagag cgauacccag      840

uauguaugca aaaggacuuu gguggaccgg ggauggggca augguugugg gcuguucgga      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaaaaugac cggcaagucc      960

auucagccug aaaaccucga guaccguauc augcugagcg ugcacggguc gcaacacucu     1020

ggaaugauug uuaaugauac gggucaugaa accgacgaga accgcgcuaa gguggagauc     1080

acaccaaauu cccccagagc cgaagcuacu cuuggcgggu ucggaucacu gggcuuggau     1140

ugugagccua ggaccggucu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gacaucccac ucccguggca cgccggggca     1260

gauacuggca caccccauug gaacaauaaa gaggcccuug uggaauuuaa ggacgcucac     1320

gcgaaacggc agaccguagu ugugcuggga agccaggagg gcgcagucca uaccgccuug     1380

gcuggggccc uagaggcaga aauggaugga gccaaggguc gacuguccuc uggccaccuc     1440

aaaugccgcc ugaagaugga caaacuuaga cugaaggggg ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gagacccucc auggaacugu caccguggaa     1560

guucaguacg cgggcacaga cgguccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg gagguuaaua acagcaaacc cagugaucac ggagaguacc     1680

gaaaacucca agaugaugcu ggagcuagau cccccguucg gcgacagcua cauuguuauc     1740

ggagugggcg agaaaaagau aacucaccac uggcaucggu cugggucaac aauuggaaag     1800

gccuuugaag cuaccguccg cggugccaaa agaauggcag ugcucggcga cacagcuugg     1860

gauuucggga guguaggagg cgcccugaau agccuuggua aggggaucca ccagaucuuc     1920

ggcgcggccu uuaaauccuu guucggaggc augucuuggu uuucgcaaau ucugaucggg     1980

acucuccuga uguggcuugg acugaacacc aagaauggca gcauuuccuu gaugugucuc     2040

gcucugggug ggguguuaau auuccuguca acggcaguua gugccgacgu cggaugcucu     2100

guggauuuua gcaaaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3882
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3882
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu uacaacugcc       60

auggcugcag agguuacgag aaggggaucc gccuacuaua uguaccugga ucggaacgac      120

gcgggugaag cuauuucuuu ccccaccaca uugggcauga auaagugcua uauacagauc      180

auggauuuag ggcacaugug ugacgccacu augucauacg agugcccuau gcuggaugaa      240

ggaguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

ggcacuuguc aucacaaaaa gggugaagca cgccgaagcc guagagccgu gacacuaccg      360

ucccauucua ccaggaaacu ccaaacgcgg ucgcagacuu ggcuggaguc acgcgaguac      420

accaagcacc uuauuagagu ugaaaauugg aucuuuagga accccggguu cgcucuggca      480

gccgcugcca uugcgugguu gcucggaagc aguacauccc agaaagugau cuaucugguc      540

augauacugc ucaucgcacc cgccuacucu auucggugca ucggcgugag caaucgcgau      600

uuuguagagg gcauguccgg gggaacuugg guugacauug uguuagaaca ugguggcugu      660

gucaccguga uggcucagga uaagccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg ccgaggugcg aaguuacugc uaugaagcaa gcauaucuga uauggcuucc      780

gacucaagau gucccacaca aggggaggcc uaccuugaca agcagagcga uacccaguau      840

guaugcaaaa ggacuuuggu ggaccgggga uggggcaaug guugugggcu guucggaaag      900

ggcagucuag ucacaugcgc gaaauuugcc ugcucuaaga aaaugaccgg caaguccauu      960

cagccugaaa accucgagua ccguaucaug cugagcgugc acgggucgca acacucugga     1020

augauuguua augauacggg ucaugaaacc gacgagaacc gcgcaaaggu ggagaucaca     1080

ccaaauuccc ccagagcuga agccacucuu ggcggguucg gaucacuggg cuuggauugu     1140

gagccuagga ccggucucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc uggggccgau     1260

acuggcaccc cucauuggaa caauaaagag gcacuugugg aauuuaagga cgcccacgcu     1320

aaacggcaga caguaguugu gcugggaagc caggagggcg cgguccauac cgcauuggcc     1380

ggggcucuag aggccgaaau ggauggagca aagggucgac uguccucugg ccaccucaaa     1440

ugccgccuga agauggacaa acuuagacug aaggggguga gcuauucauu guguacugcc     1500

gcuuucacgu uuacaaagau uccugccgag acccuccaug gaacugucac cguggaaguu     1560

caguacgcag gcacagacgg uccaugcaaa gugcccgcgc aaauggcugu agauaugcag     1620

acccugacuc cugucgggag guuaauaaca gccaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggcg acagcuacau uguuaucgga     1740

gugggcgaga aaaagauaac ucaccacugg caucggucug ggucaacaau uggaaaggca     1800

uuugaagcca ccguccgcgg ugcuaaaaga auggccgugc ucggcgacac agcaugggau     1860

uucgggagug uaggaggcgc ucugaauagc cuugguaagg ggauccacca gaucuucggc     1920

gccgcguuua aauccuuguu cggaggcaug ucuugguuuu cgcaaauucu gaucgggacu     1980

cuccugaugu ggcuuggacu gaacaccaag aauggcagca uuuccuugau gugucucgcc     2040

cugggugggg uguuaauauu ccugucaacg gcuguuagug cagacgucgg augcucugug     2100

gauuuuagca aaugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3883
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3883
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcag augucggaug cucuguggac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3884
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3884
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagccg uuacgagaag gggauccgcg uacuauaugu accuggaccg gaacgaugcu      120

ggugaggcca uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcaacuaug ucauacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaggcccgc cgaagccgua gagcugugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggaaucacg cgaguacacc      420

aagcaccuua uuagaguuga gaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagaaggca uguccggggg aacuuggguu gauauugugu uagagcaugg uggcuguguc      660

accgugaugg cccaggacaa gccaacagug gauaucgaac uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaggcuagca uaucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaagcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugagaacc ucgaauaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugagaccgau gaaaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaggc uacucuuggc ggguucggau cacugggcuu ggacugugaa     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggagugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacacu     1260

ggcacacccc auuggaacaa uaaagaagcc cuuguggagu uuaaggaugc ucacgcgaaa     1320

cggcagaccg uaguugugcu gggaagccag gaaggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagagaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagaaacc cuccauggaa cugucaccgu ggagguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaggcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccg augucggaug cucuguggac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3885
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3885
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccgc gguuacgagg cgggguuccg cuuacuauau guaccuggau      120

cgcaacgacg ccggcgaggc aauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgccacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaggcuc gacguagcag aagggcagug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaauca      420

agagaguaca ccaagcaccu uauuaggguu gagaauugga ucuuucggaa ccccggauuc      480

gcccuggcug ccgcggcaau ugccugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgcuccu gccuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcacaggau aagccaacag uggacaucga acuggucacc      720

acgacuguuu caaacauggc ugaggugaga aguuacugcu augaggccag cauaucugau      780

auggcguccg acucaaggug ucccacacaa ggagaagccu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgca aaauuugcuu gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgaaaacag agccaaggug     1080

gagaucacac caaauucccc cagggcugag gccacucuug ggggauucgg cucacugggu     1140

uuggauugug aaccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgca     1260

ggcgccgaua cuggaacacc ccauuggaac aauaaagaag cucuugugga guuuaaggac     1320

gcgcacgcaa aacgacagac cguaguugug cugggcagcc aggaaggggc cguccauacc     1380

gcuuuggccg gagcacuaga ggccgagaug gauggugcua agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugccg cauucacguu uacaaagauu ccugcggaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcugg uacagacggg ccaugcaaag ugcccgccca aauggcagua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag ccaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggaa cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggcuu uugaggccac cgucagaggc gcaaaaagga uggcugugcu cggggacaca     1860

gccugggauu ucggaagugu aggcggugcg cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag ccgcuuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcac uugggggagu guuaauauuc cugucaacgg ccguuagugc ugacgucggc     2100

ugcucugugg auuuuagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3886
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3886
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagguguuac ucgacguggc      120

ucagccuacu auauguaccu ggauagaaac gacgcugggg aggcaauuag uuuucccacg      180

acccuaggaa ugaauaaaug cuauauacaa aucauggauc ucggccauau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggugugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugggaccu gucaccauaa gaaaggagag      360

gcgaggcggu cucgcagagc ugugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg cuuugcccug gcagccgcug caauugccug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc uccugccuac      600

uccauuaggu gcaucggggu gucaaaucgg gauuucguag aaggaaugag ugguggcaca      660

uggguugaca uuguguugga gcacggggga ugugucacug ugauggcgca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggcagaggu gcguucuuac      780

ugcuaugagg ccuccauauc agauauggcu agcgacaguc gcugucccac ucagggcgaa      840

gccuaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

gguuggggga auggaugugg ccuguuuggc aaaggguccc uugucacuug cgcaaaguuc      960

gcuugcagca aaaagaugac aggaaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacgguuc ccagcauuca ggcaugauug uuaaugauac cgggcacgag     1080

acggacgaaa accgagccaa aguggagauc accccaaaua guccccgcgc ggaggccaca     1140

cucggaggcu uugguagccu gggguuagau ugugaaccua gaacuggccu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcaggagcu gauacaggca cuccccacug gaacaauaag     1320

gaagcccuag uggaguucaa agacgcucau gccaagaggc agaccguagu ugugcugggg     1380

ucucaggaag gagcagucca cacagcccuc gcuggugcgc uggaggcaga gauggauggc     1440

gccaaagggc ggcuuagcuc aggacaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggcg ugaguuauuc cuuauguacc gcugccuuua cuuucacgaa aauuccugca     1560

gaaacacugc acgguaccgu cacuguggag guucaauacg ccgggaccga cggcccaugc     1620

aaggugcccg cucagauggc cguagauaug cagacacuaa ccccugucgg aaggcucaua     1680

acugcaaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gcgacucaua cauuguuauc ggggugggag agaagaagau aacccaccau     1800

uggcaccgua gugguagcac uauuggcaaa gcguucgagg cuacaguccg aggggccaag     1860

cggauggcag uguugggaga caccgccugg gauuucggcu ccguaggugg ggcucucaau     1920

ucucugggca aaggaaucca ucaaaucuuu ggcgccgcau ucaagucgcu guuuggggga     1980

augagcuggu ucucccagau ucuuaucggc acacuguuga uguggcucgg ucugaacacu     2040

aaaaaugggu caauuaguuu aaugugucug gcucuaggag gcgugcucau auuucugucu     2100

accgccguua gcgcggacgu cgguugcucc guggauuucu caaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3887
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3887
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagccguuac ucgacguggu      120

ucagcuuacu auauguaccu ggauagaaac gacgcaggcg aggccauuag uuuucccacg      180

acccuaggga ugaauaaaug cuauauacaa aucauggauc ucggacauau gugugacgcg      240

acaaugagcu acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugguaccu gucaccauaa gaaaggggag      360

gcuaggcggu cucgcagagc cgugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg auuugcacug gccgcugcag ccauugcuug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc cccugcguac      600

uccauuaggu gcaucggcgu gucaaaucgg gauuucguag aagggaugag uggagguaca      660

uggguugaca uuguguugga gcacggcggg ugugucacug ugauggcaca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggccgaggu gcguucuuac      780

ugcuaugagg cuuccauauc agauauggcc agcgacaguc gcugucccac ucagggagaa      840

gcauaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

ggcuggggua augggugugg acuguuuggc aaaggcuccc uugucacuug cgcuaaguuc      960

gccugcagca aaaagaugac agggaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacggauc ccagcauuca gguaugauug uuaaugauac cggccacgag     1080

acggacgaaa accgagcgaa aguggagauc accccaaaua guccccgcgc cgaggcaaca     1140

cucgggggau uuggcagccu ggguuuagau ugugaaccua gaacugggcu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcuggcgcc gauacaggaa cuccccacug gaacaauaag     1320

gaagcucuag uggaguucaa agacgcccau gcaaagaggc agaccguagu ugugcugggc     1380

ucucaggaag gggccgucca cacagcucuc gcgggagcac uggaggccga gauggauggu     1440

gcuaaaggcc ggcuuagcuc agggcaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggag ugaguuauuc cuuauguacc gccgcauuua cuuucacgaa aauuccugcc     1560

gaaacacugc acggcaccgu cacuguggag guucaauacg cugguaccga cgggccaugc     1620

aaggugcccg cccagauggc aguagauaug cagacacuaa ccccugucgg caggcucaua     1680

acugcgaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gagacucaua cauuguuauc ggcguggggg agaagaagau aacccaccau     1800

uggcaccgua guggaagcac uauugguaag gcuuucgagg ccacaguccg gggcgcaaaa     1860

cgaauggccg uguuggggga caccgcuugg gauuucggau ccguaggcgg ugcccucaau     1920

ucucugggga aaggcaucca ucaaaucuuu ggagcagcuu ucaagucgcu guuuggcggg     1980

augagcuggu ucucccagau ucuuaucgga acacuguuga uguggcucgg ccugaacacu     2040

aaaaaugguu caauuaguuu aaugugucug gcccuagggg gagugcucau auuucugucu     2100

accgcgguua gcgccgacgu cggcugcucc guggauuucu caaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  3888
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3888
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3889
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3889
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagaggcg gggcgcugac       60

accagcgugg ggauagucgg acugcuccuu acaacugcaa uggccgcgga aguuacgcgc      120

cgagguuccg cuuacuauau guaccuggau cguaaugacg ccggcgaggc aaucucuuuc      180

cccaccacau uggggaugaa caaaugcuau auucagauca uggauuuagg acacaugugu      240

gacgccacua ugucauacga augcccuaug cuggaugagg gcguggaacc agacgaugua      300

gacuguuggu gcaauaccac aaguaccugg guggucuaug guacuuguca ucacaagaaa      360

ggggaggcua gaaggagccg gcgcgcagug acacuaccgu cccauucuac cagaaagcuc      420

caaacgaggu cgcagacuug gcuggaguca cgggaauaca ccaagcaccu uauucgcguu      480

gagaacugga ucuuucgaaa ucccggauuc gcccuggcug ccgcggcaau agccugguug      540

cucggcagca guacauccca gaaagugauc uaucugguca ugauucugcu caucgcuccu      600

gccuacucua uuagaugcau cggcgugagc aacagggauu uuguagaagg gauguccgga      660

gguacuuggg uugacauagu guuagagcau ggcgggugug ucaccgugau ggcacaggau      720

aagccaacag uggacauuga gcuggucacc acgacuguuu caaauauggc ugaagugcgg      780

aguuacugcu augaggccag caucucugau auggcguccg acucacguug ucccacacaa      840

ggagaagccu accuugacaa acagagcgau acccaguaug uaugcaagcg cacuuuggug      900

gacagaggcu gggguaacgg guguggacug uucggcaaag gcagucuagu cacaugcgca      960

aaguuugcuu gcucuaagaa aaugaccggg aaguccauuc agccugagaa ucucgaauac     1020

aggaucaugc ugagcgugca cggaucgcaa cacucuggua ugaucguuaa cgauacgggc     1080

caugagaccg acgagaaucg ggccaaagug gaaauuacac caaacucacc ccgagcugag     1140

gccacucuug ggggauucgg cucccugggu uuggauugug aaccucgcac cgggcucgac     1200

uuuagugauc uguauuacuu aacaaugaau aacaagcacu ggcuggucca uaaagagugg     1260

uuccacgaca uaccacuccc guggcacgca ggcgccgaua cuggaacacc ccauuggaau     1320

aacaaggaag cucuugugga guuuaaagac gcgcacgcaa agagacagac cguaguugug     1380

cugggcagcc aggagggggc cguccauacc gcuuuggccg gagcacuaga agccgagaug     1440

gauggugcua agggcaggcu guccucuggg caccucaaau gccggcugaa gauggacaaa     1500

cuucgccuga agggagugag cuauucauug uguacugccg cauucacguu uacaaaaauc     1560

ccugcggaaa cccuccaugg cacugucacc guggagguuc aguacgcugg uacagacggg     1620

ccaugcaagg ugcccgccca aauggcagua gauaugcaga cccugacucc ugucggcaga     1680

uuaauuacag ccaacccagu gaucacggaa aguaccgaaa auuccaagau gaugcuggag     1740

cuagaucccc cguucggaga cagcuacaua guuauuggcg ugggggagaa aaagaucacu     1800

caccacuggc auaggucugg aucaacaauc gguaaggcuu uugaggccac cguccguggc     1860

gcaaaacgga uggcugugcu cggggacaca gccugggauu ucggaagugu aggcggugcg     1920

cugaacagcc uugggaaagg cauucaccag aucuucggag ccgcuuuuaa guccuuguuc     1980

ggcgggaugu cuugguuuuc gcaaauucug auaggaacuc uccugaugug gcuuggccug     2040

aauaccaaga acgguagcau cuccuugaug ugucucgcau uagggggagu gcugauuuuc     2100

cugucaacgg ccguuagugc ugacgucggc ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  3890
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3890
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agaggcgggg cgcugacacc       60

agcgugggga uugucggacu gcuccuuaca acugcaaugg ccgcggaagu uacgcgccga      120

gguuccgcuu acuauaugua ccuggaucgu aaugacgccg gcgaggcaau aucuuucccc      180

accacauugg ggaugaacaa augcuauauc cagauuaugg auuuaggaca caugugugac      240

gccacuaugu cauacgaaug cccuaugcug gaugagggcg uggaaccaga cgauguagac      300

uguuggugca auaccacaag uaccugggug gucuauggua cuugucauca caagaaaggg      360

gaggcuagaa ggagccggcg cgcagugaca cuaccguccc auucuaccag aaagcuccaa      420

acgaggucgc agacuuggcu ggagucacgg gaauacacca agcaccuuau ccgcguugag      480

aacuggauuu uucgaaaucc cggauucgcc cuggcugccg cggcaaucgc cugguugcuc      540

ggcagcagua caucccagaa agugauauau cuggucauga uccugcucau ugcuccugcc      600

uacucuauca gaugcauugg cgugagcaac agggauuuug uagaagggau guccggaggu      660

acuuggguug acaucguguu agagcauggc ggguguguca ccgugauggc acaggauaag      720

ccaacagugg acauagagcu ggucaccacg acuguuucaa auauggcuga agugcggagu      780

uacugcuaug aggccagcau uucugauaug gcguccgacu cacguugucc cacacaagga      840

gaagccuacc uugacaaaca gagcgauacc caguauguau gcaagcgcac uuugguggac      900

agaggcuggg guaacgggug uggacuguuc ggcaaaggca gucuagucac augcgcaaag      960

uuugcuugcu cuaagaaaau gaccgggaag uccauccagc cugagaaucu cgaauacagg     1020

auuaugcuga gcgugcacgg aucgcaacac ucugguauga ucguuaacga uacgggccau     1080

gagaccgacg agaaucgggc caaaguggaa aucacaccaa acucaccccg agcugaggcc     1140

acucuugggg gauucggcuc ccuggguuug gauugugaac cucgcaccgg gcucgacuuu     1200

agugaucugu auuacuuaac aaugaauaac aagcacuggc ugguccauaa agagugguuc     1260

cacgacauuc cacucccgug gcacgcaggc gccgauacug gaacacccca uuggaauaac     1320

aaggaagcuc uuguggaguu uaaagacgcg cacgcaaaga gacagaccgu aguugugcug     1380

ggcagccagg agggggccgu ccauaccgcu uuggccggag cacuagaagc cgagauggau     1440

ggugcuaagg gcaggcuguc cucugggcac cucaaaugcc ggcugaagau ggacaaacuu     1500

cgccugaagg gagugagcua uucauugugu acugccgcau ucacguuuac aaaaauaccu     1560

gcggaaaccc uccauggcac ugucaccgug gagguucagu acgcugguac agacgggcca     1620

ugcaaggugc ccgcccaaau ggcaguagau augcagaccc ugacuccugu cggcagauua     1680

aucacagcca acccagugau uacggaaagu accgaaaauu ccaagaugau gcuggagcua     1740

gaucccccgu ucggagacag cuacaucguu auaggcgugg gggagaaaaa gauuacucac     1800

cacuggcaua ggucuggauc aacaaucggu aaggcuuuug aggccaccgu ccguggcgca     1860

aaacggaugg cugugcucgg ggacacagcc ugggauuucg gaaguguagg cggugcgcug     1920

aacagccuug ggaaaggcau ccaccagauu uucggagccg cuuuuaaguc cuuguucggc     1980

gggaugucuu gguuuucgca aauccugauu ggaacucucc ugauguggcu uggccugaau     2040

accaagaacg guagcauauc cuugaugugu cucgcauuag ggggagugcu gaucuuccug     2100

ucaacggccg uuagugcuga cgucggcugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  3891
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3891
gggagaaagc uuaccaugaa cgccagaaag gagaaaaaga ggcggggcgc ugacaccagc       60

guggggaucg ucggacugcu ccuuacaacu gcaauggccg cggaaguuac gcgccgaggu      120

uccgcuuacu auauguaccu ggaucguaau gacgccggcg aggcaauuuc uuuccccacc      180

acauugggga ugaacaaaug cuauauacag aucauggauu uaggacacau gugugacgcc      240

acuaugucau acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaua ccacaaguac cugggugguc uaugguacuu gucaucacaa gaaaggggag      360

gcuagaagga gccggcgcgc agugacacua ccgucccauu cuaccagaaa gcuccaaacg      420

aggucgcaga cuuggcugga gucacgggaa uacaccaagc accuuauucg cguugagaac      480

uggaucuuuc gaaaucccgg auucgcccug gcugccgcgg caauugccug guugcucggc      540

agcaguacau cccagaaagu gaucuaucug gucaugauac ugcucaucgc uccugccuac      600

ucuauuagau gcaucggcgu gagcaacagg gauuuuguag aagggauguc cggagguacu      660

uggguugaca uuguguuaga gcauggcggg ugugucaccg ugauggcaca ggauaagcca      720

acaguggaca ucgagcuggu caccacgacu guuucaaaua uggcugaagu gcggaguuac      780

ugcuaugagg ccagcauauc ugauauggcg uccgacucac guugucccac acaaggagaa      840

gccuaccuug acaaacagag cgauacccag uauguaugca agcgcacuuu gguggacaga      900

ggcuggggua acgggugugg acuguucggc aaaggcaguc uagucacaug cgcaaaguuu      960

gcuugcucua agaaaaugac cgggaagucc auucagccug agaaucucga auacaggauc     1020

augcugagcg ugcacggauc gcaacacucu gguaugauug uuaacgauac gggccaugag     1080

accgacgaga aucgggccaa aguggaaauc acaccaaacu caccccgagc ugaggccacu     1140

cuugggggau ucggcucccu ggguuuggau ugugaaccuc gcaccgggcu cgacuuuagu     1200

gaucuguauu acuuaacaau gaauaacaag cacuggcugg uccauaaaga gugguuccac     1260

gacaucccac ucccguggca cgcaggcgcc gauacuggaa caccccauug gaauaacaag     1320

gaagcucuug uggaguuuaa agacgcgcac gcaaagagac agaccguagu ugugcugggc     1380

agccaggagg gggccgucca uaccgcuuug gccggagcac uagaagccga gauggauggu     1440

gcuaagggca ggcuguccuc ugggcaccuc aaaugccggc ugaagaugga caaacuucgc     1500

cugaagggag ugagcuauuc auuguguacu gccgcauuca cguuuacaaa aauuccugcg     1560

gaaacccucc auggcacugu caccguggag guucaguacg cugguacaga cgggccaugc     1620

aaggugcccg cccaaauggc aguagauaug cagacccuga cuccugucgg cagauuaaua     1680

acagccaacc cagugaucac ggaaaguacc gaaaauucca agaugaugcu ggagcuagau     1740

cccccguucg gagacagcua cauuguuauc ggcguggggg agaaaaagau aacucaccac     1800

uggcauaggu cuggaucaac aauugguaag gcuuuugagg ccaccguccg uggcgcaaaa     1860

cggauggcug ugcucgggga cacagccugg gauuucggaa guguaggcgg ugcgcugaac     1920

agccuuggga aaggcaucca ccagaucuuc ggagccgcuu uuaaguccuu guucggcggg     1980

augucuuggu uuucgcaaau ucugaucgga acucuccuga uguggcuugg ccugaauacc     2040

aagaacggua gcauuuccuu gaugugucuc gcauuagggg gagugcugau auuccuguca     2100

acggccguua gugcugacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3892
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3892
gggagaaagc uuaccauggc cagaaaggag aaaaagaggc ggggcgcuga caccagcgug       60

gggaucgucg gacugcuccu uacaacugca auggccgcgg aaguuacgcg ccgagguucc      120

gcuuacuaua uguaccugga ucguaacgac gccggcgagg caauuucuuu ccccaccaca      180

uuggggauga auaaaugcua uauacagauc auggauuuag gacacaugug ugacgccacu      240

augucauacg aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg      300

ugcaacacca caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu      360

agaaggagcc ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccaaacgagg      420

ucgcagacuu ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaauugg      480

aucuuucgaa accccggauu cgcccuggcu gccgcggcaa uugccugguu gcucggcagc      540

aguacauccc agaaagugau cuaucugguc augauacugc ucaucgcucc ugccuacucu      600

auuagaugca ucggcgugag caauagggau uuuguagaag ggauguccgg agguacuugg      660

guugacauug uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca      720

guggacaucg agcuggucac cacgacuguu ucaaacaugg cugaagugcg gaguuacugc      780

uaugaggcca gcauaucuga uauggcgucc gacucacguu gucccacaca aggagaagcc      840

uaccuugaca aacagagcga uacccaguau guaugcaagc gcacuuuggu ggacagaggc      900

ugggguaaug gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu      960

ugcucuaaga aaaugaccgg gaaguccauu cagccugaga accucgaaua caggaucaug     1020

cugagcgugc acggaucgca acacucuggu augauuguua augauacggg ccaugagacc     1080

gacgagaacc gggccaaagu ggaaaucaca ccaaauucac cccgagcuga ggccacucuu     1140

gggggauucg gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau     1200

cuguauuacu uaacaaugaa caauaagcac uggcuggucc auaaagagug guuccacgac     1260

aucccacucc cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa     1320

gcucuugugg aguuuaaaga cgcgcacgca aagagacaga ccguaguugu gcugggcagc     1380

caggaggggg ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu     1440

aagggcaggc uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcauucacgu uuacaaaaau uccugcggaa     1560

acccuccaug gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag     1620

gugcccgccc aaauggcagu agauaugcag acccugacuc cugucggcag auuaauaaca     1680

gccaacccag ugaucacgga aaguaccgag aacuccaaga ugaugcugga gcuagauccc     1740

ccguucggag acagcuacau uguuaucggc gugggggaga aaaagauaac ucaccacugg     1800

cauaggucug gaucaacaau ugguaaggcu uuugaagcca ccguccgugg cgcaaaacgg     1860

auggcugugc ucggggacac agccugggau uucggaagug uaggcggugc gcugaauagc     1920

cuugggaaag gcauccacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug     1980

ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag     2040

aaugguagca uuuccuugau gugucucgca uuagggggag ugcugauauu ccugucaacg     2100

gccguuagug cugacgucgg cugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3893
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3893
gggagaaagc uuaccaugag aaaggagaaa aagaggcggg gcgccgacac cagcgugggg       60

aucgucggac ugcuccuuac aacugcuaug gcagccgaag uuacgcgccg agguuccgcg      120

uacuauaugu accuggaucg uaacgacgcu ggcgaggcca uuucuuuccc caccacauug      180

gggaugaaua aaugcuauau acagaucaug gauuuaggac acauguguga cgcaacuaug      240

ucauacgaau gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc      300

aacaccacaa guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggccaga      360

aggagccggc gcgcugugac acuaccgucc cauucuacca gaaagcucca aacgaggucg      420

cagacuuggc uggagucacg ggaauacacc aagcaccuua uucgcguuga gaauuggauc      480

uuucgaaacc ccggauucgc acuggccgcu gccgcgauug caugguugcu cggcagcagu      540

acaucccaga aagugaucua ucuggucaug auacugcuca ucgccccugc uuacucuauu      600

agaugcaucg gcgugagcaa uagggauuuu guagaaggga uguccggagg uacuuggguu      660

gacauugugu uagagcaugg cggguguguc accgugaugg cccaggauaa gccaacagug      720

gacaucgagc uggucaccac gacuguuuca aacauggcag aagugcggag uuacugcuau      780

gaggcuagca uaucugauau ggccuccgac ucacguuguc ccacacaagg agaagcguac      840

cuugacaaac agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg      900

gguaaugggu guggacuguu cggcaaaggc agucuaguca caugcgccaa guuugcaugc      960

ucuaagaaaa ugaccgggaa guccauucag ccugagaacc ucgaauacag gaucaugcug     1020

agcgugcacg gaucgcaaca cucugguaug auuguuaaug auacgggcca ugagaccgac     1080

gagaaccggg cuaaagugga aaucacacca aauucacccc gagccgaggc uacucuuggg     1140

ggauucggcu cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug     1200

uauuacuuaa caaugaacaa uaagcacugg cugguccaua aagagugguu ccacgacauc     1260

ccacucccgu ggcacgccgg cgcagauacu ggaacacccc auuggaacaa uaaggaagcc     1320

cuuguggagu uuaaagacgc ucacgcgaag agacagaccg uaguugugcu gggcagccag     1380

gagggggcag uccauaccgc cuuggcugga gcccuagaag cagagaugga uggugccaag     1440

ggcaggcugu ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag     1500

ggagugagcu auucauugug uacugcugcc uucacguuua caaaaauucc ugcagaaacc     1560

cuccauggca cugucaccgu ggagguucag uacgcgggua cagacgggcc augcaaggug     1620

cccgcucaaa uggccguaga uaugcagacc cugacuccug ucggcagauu aauaacagca     1680

aacccaguga ucacggaaag uaccgagaac uccaagauga ugcuggagcu agaucccccg     1740

uucggagaca gcuacauugu uaucggcgug ggggagaaaa agauaacuca ccacuggcau     1800

aggucuggau caacaauugg uaaagccuuu gaagcuaccg uccguggcgc caagcggaug     1860

gcagugcucg gggacacagc uugggauuuc ggaaguguag gcggugcccu gaauagccuu     1920

gggaaaggca uccaccagau cuucggagcg gccuuuaagu ccuuguucgg cgggaugucu     1980

ugguuuucgc aaauucugau cggaacucuc cugauguggc uuggccugaa caccaagaau     2040

gguagcauuu ccuugaugug ucucgcuuua gggggagugc ugauauuccu gucaacggca     2100

guuagugccg acgucggcug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3894
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3894
gggagaaagc uuaccaugaa ggagaaaaag agaaggggcg ccgacaccag cguggggauc       60

gucggacugc uccuuacaac ugcuauggca gccgaaguua cgcggcgcgg uuccgcguac      120

uauauguacc uggaucgaaa cgacgcuggc gaggccauuu cuuuccccac cacauugggg      180

augaauaaau gcuauauaca gaucauggau uuaggacaca ugugugacgc aacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcccguaga      360

agcaggcggg cugugacacu accgucccau ucuacccgca agcuccaaac gagaucgcag      420

acuuggcugg agucaaggga auacaccaag caccuuauuc ggguugagaa uuggaucuuu      480

cgcaaccccg gauucgcacu ggccgcugcc gcgauugcau gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugaua cugcucaucg ccccugcuua cucuauucga      600

ugcaucggcg ugagcaauag agauuuugua gaagggaugu ccggagguac uuggguugac      660

auuguguuag agcauggcgg gugugucacc gugauggccc aggauaagcc aacaguggac      720

aucgagcugg ucaccacgac uguuucaaac auggcagaag ugaggaguua cugcuaugag      780

gcuagcauau cugauauggc cuccgacuca cgguguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guauguaugc aagcguacuu ugguggaccg cggcuggggu      900

aaugggugug gacuguucgg caaaggcagu cuagucacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaccucg aauacagaau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauu guuaaugaua cgggccauga gaccgacgag     1080

aacagggcua aaguggaaau cacaccaaau ucaccccggg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagcgc cagaccguag uugugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

agacuguccu cugggcaccu caaaugcagg cugaagaugg acaaacuucg gcugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauuccugc agaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gccgcuuaau aacagcaaac     1680

ccagugauca cggaaaguac cgagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauuguuau cggcgugggg gagaaaaaga uaacucacca cuggcauaga     1800

ucuggaucaa caauugguaa agccuuugaa gcuaccguca ggggcgccaa gcguauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

agcauuuccu ugaugugucu cgcuuuaggg ggagugcuga uauuccuguc aacggcaguu     2100

agugccgacg ucggcugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3895
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3895
gggagaaagc uuaccaugga gaagaaaaga aggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc uuacaacugc uauggcagcc gaaguuacgc ggcgcgguuc cgcguacuau      120

auguaccugg aucgaaacga cgcuggcgag gccauuucuu uccccaccac auuggggaug      180

aauaagugcu auauacagau cauggauuua ggacacaugu gugacgcaac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaaa agggggaggc ccguagaagc      360

aggcgggcug ugacacuacc gucccauucu acccgcaaac uccaaacgag aucgcagacu      420

uggcuggagu caagggaaua caccaagcac cuuauucggg uugagaauug gaucuuucgc      480

aaccccggau ucgcacuggc cgcugccgcg auugcauggu ugcucggcag caguacaucc      540

cagaagguga ucuaucuggu caugauacug cucaucgccc cugcuuacuc uauucgaugc      600

aucggcguga gcaauagaga uuuuguagaa gggauguccg gagguacuug gguugacauu      660

guguuagagc auggcgggug ugucaccgug auggcccagg auaaaccaac aguggacauc      720

gagcugguca ccacgacugu uucaaacaug gcagaaguga ggaguuacug cuaugaggcu      780

agcauaucug auauggccuc cgacucacgg ugucccacac aaggagaagc guaccuugac      840

aagcagagcg auacccagua uguaugcaaa cguacuuugg uggaccgcgg cugggguaau      900

ggguguggac uguucggcaa gggcagucua gucacaugcg ccaaauuugc augcucuaag      960

aagaugaccg ggaaauccau ucagccugag aaccucgaau acagaaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugauuguu aaugauacgg gccaugagac cgacgagaac     1080

agggcuaagg uggaaaucac accaaauuca ccccgggccg aggcuacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucga accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga acaauaaaca cuggcugguc cauaaggagu gguuccacga caucccacuc     1260

ccguggcacg ccggcgcaga uacuggaaca ccccauugga acaauaaaga agcccuugug     1320

gaguuuaagg acgcucacgc gaaacgccag accguaguug ugcugggcag ccaggagggg     1380

gcaguccaua ccgccuuggc uggagcccua gaagcagaga uggauggugc caagggcaga     1440

cuguccucug ggcaccucaa gugcaggcug aaaauggaca agcugcggcu gaaaggagug     1500

agcuauucau uguguacugc ugccuucacg uuuacaaaga uuccugcaga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcg gguacagacg ggccaugcaa agugcccgcu     1620

caaauggccg uagauaugca gacccugacu ccugucggcc gcuuaauaac agcaaaccca     1680

gugaucacgg aaaguaccga gaacuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uuguuaucgg cgugggggaa aagaaaauaa cucaccacug gcauagaucu     1800

ggaucaacaa uugguaaggc cuuugaggcu accgucaggg gcgccaaacg uauggcagug     1860

cucggggaca cagcuuggga uuucggaagu guaggcggug cccugaauag ccuugggaag     1920

ggcauccacc agaucuucgg agcggccuuu aaauccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugaucggaac ucuccugaug uggcuuggcc ugaacaccaa gaaugguagc     2040

auuuccuuga ugugucucgc ucuuggggga guguuaauau uccugucaac ggcaguuagu     2100

gccgacgucg gcugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3896
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3896
gggagaaagc uuaccaugaa gaaaagaagg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuua caacugcuau ggcagccgag guuacgcggc gcgguuccgc guacuauaug      120

uaccuggauc gaaacgacgc uggcgaagcc auuucuuucc ccaccacauu ggggaugaau      180

aagugcuaua uacagaucau ggauuuagga cacaugugug acgcaacuau gucauacgag      240

ugcccuaugc uggaugaagg cguggagcca gacgauguag acuguuggug caacaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaaaaagg gggaagcccg uagaagcagg      360

cgggcuguga cacuaccguc ccauucuacc cgcaaacucc aaacgagauc gcagacuugg      420

cuggagucaa gggaguacac caagcaccuu auucggguug aaaauuggau cuuucgcaac      480

cccggauucg cacuggccgc ugccgcgauu gcaugguugc ucggcagcag uacaucccag      540

aaggugaucu aucuggucau gauacugcuc aucgccccug cuuacucuau ucgaugcauc      600

ggcgugagca auagagauuu uguagagggg auguccggag guacuugggu ugacauugug      660

uuagaacaug gcgggugugu caccgugaug gcccaggaua aaccaacagu ggacaucgag      720

cuggucacca cgacuguuuc aaacauggca gaggugagga guuacugcua ugaagcuagc      780

auaucugaua uggccuccga cucacggugu cccacacaag gagaggcgua ccuugacaag      840

cagagcgaua cccaguaugu augcaaacgu acuuuggugg accgcggcug ggguaauggg      900

uguggacugu ucggcaaggg cagucuaguc acaugcgcca aauuugcaug cucuaagaag      960

augaccggga aauccauuca gccugaaaac cucgaguaca gaaucaugcu gagcgugcac     1020

ggaucgcaac acucugguau gauuguuaau gauacgggcc augaaaccga cgagaacagg     1080

gcuaaggugg agaucacacc aaauucaccc cgggccgaag cuacucuugg gggauucggc     1140

ucccuggguu uggauuguga gccucgaacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgacau cccacucccg     1260

uggcacgccg gcgcagauac uggaacaccc cauuggaaca auaaagaggc ccuuguggaa     1320

uuuaaggacg cucacgcgaa acgccagacc guaguugugc ugggcagcca ggagggggca     1380

guccauaccg ccuuggcugg agcccuagag gcagaaaugg auggugccaa gggcagacug     1440

uccucugggc accucaagug caggcugaaa auggacaagc ugcggcugaa aggagugagc     1500

uauucauugu guacugcugc cuucacguuu acaaagauuc cugcagagac ccuccauggc     1560

acugucaccg uggaaguuca guacgcgggu acagacgggc caugcaaagu gcccgcucaa     1620

auggccguag auaugcagac ccugacuccu gucggccgcu uaauaacagc aaacccagug     1680

aucacggaga guaccgaaaa cuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacauug uuaucggcgu gggggagaag aaaauaacuc accacuggca uagaucugga     1800

ucaacaauug guaaggccuu ugaagcuacc gucaggggcg ccaaacguau ggcagugcuc     1860

ggggacacag cuugggauuu cggaagugua ggcggugccc ugaauagccu ugggaagggc     1920

auccaccaga ucuucggagc ggccuuuaaa uccuuguucg gcgggauguc uugguuuucg     1980

caaauucuga ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu     2040

uccuugaugu gucucgcucu ugggggagug uuaauauucc ugucaacggc aguuagugcc     2100

gacgucggcu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3897
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3897
gggagaaagc uuaccaugaa gagaaggggc gccgacacca gcguggggau cgucggacug       60

cuccuuacaa cugcuauggc agccgagguu acgcggcgcg guuccgcgua cuauauguac      120

cuggaucgaa acgacgcugg cgaagccauu ucuuucccca ccacauuggg gaugaauaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg caacuauguc auacgagugc      240

ccuaugcugg augaaggcgu ggagccagac gauguagacu guuggugcaa caccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aagcccguag aagcaggcgg      360

gcugugacac uaccguccca uucuacccgc aagcuccaaa cgagaucgca gacuuggcug      420

gagucaaggg aguacaccaa acaccuuauu cggguugaaa auuggaucuu ucgcaacccc      480

ggauucgcac uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaag      540

gugaucuauc uggucaugau acugcucauc gccccugcuu acucuauucg augcaucggc      600

gugagcaaua gagauuuugu agaggggaug uccggaggua cuuggguuga cauuguguua      660

gaacauggcg ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa cauggcagag gugaggaguu acugcuauga agcuagcaua      780

ucugauaugg ccuccgacuc acgguguccc acacaaggag aggcguaccu ugacaaacag      840

agcgauaccc aguauguaug caagcguacu uugguggacc gcggcugggg uaaugggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaaaaagaug      960

accgggaagu ccauucagcc ugaaaaccuc gaguacagaa ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaaugau acgggccaug aaaccgacga gaacagggcu     1080

aaaguggaga ucacaccaaa uucaccccgg gccgaagcua cucuuggggg auucggcucc     1140

cuggguuugg auugugagcc ucgaaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaacaaua agcacuggcu gguccauaaa gaaugguucc acgacauccc acucccgugg     1260

cacgccggcg cagauacugg aacaccccau uggaacaaua aggaggcccu uguggaauuu     1320

aaagacgcuc acgcgaagcg ccagaccgua guugugcugg gcagccagga gggggcaguc     1380

cauaccgccu uggcuggagc ccuagaggca gaaauggaug gugccaaagg cagacugucc     1440

ucugggcacc ucaagugcag gcugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucauugugua cugcugccuu cacguuuaca aaaauuccug cagagacccu ccauggcacu     1560

gucaccgugg aaguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug     1620

gccguagaua ugcagacccu gacuccuguc ggccgcuuaa uaacagcaaa cccagugauc     1680

acggagagua ccgaaaacuc caaaaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaagaag auaacucacc acuggcauag aucuggauca     1800

acaauuggua aagccuuuga agcuaccguc aggggcgcca agcguauggc agugcucggg     1860

gacacagcuu gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc     1920

caccagaucu ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaca ccaaaaaugg uagcauuucc     2040

uugauguguc ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac     2100

gucggcugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3898
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3898
gggagaaagc uuaccaugag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug cuauggcagc cgagguuacg cggcgcgguu ccgcguacua uauguaccug      120

gaucgaaacg acgcuggcga agccauuucu uuccccacca cauuggggau gaauaagugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgagugcccu      240

augcuggaug aaggcgugga gccagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaaa aagggggaag cccguagaag caggcgggcu      360

gugacacuac cgucccauuc uacccgcaaa cuccaaacga gaucgcagac uuggcuggag      420

ucaagggagu acaccaagca ccuuauucgg guugaaaauu ggaucuuucg caaccccgga      480

uucgcacugg ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug      600

agcaauagag auuuuguaga ggggaugucc ggagguacuu ggguugacau uguguuagaa      660

cauggcgggu gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcagaggug aggaguuacu gcuaugaagc uagcauaucu      780

gauauggccu ccgacucacg gugucccaca caaggagagg cguaccuuga caagcagagc      840

gauacccagu auguaugcaa acguacuuug guggaccgcg gcugggguaa ugggugugga      900

cuguucggca agggcagucu agucacaugc gccaaauuug caugcucuaa gaaaaugacc      960

gggaagucca uucagccuga aaaccucgag uacagaauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaaa ccgacgagaa cagggcuaag     1080

guggagauca caccaaauuc accccgggcc gaagcuacuc uugggggauu cggcucccug     1140

gguuuggauu gugagccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu ccauaaggaa ugguuccacg acaucccacu cccguggcac     1260

gccggcgcag auacuggaac accccauugg aacaauaaag aggcccuugu ggaauuuaag     1320

gacgcucacg cgaaacgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau     1380

accgccuugg cuggagcccu agaggcagaa auggauggug ccaagggcag acuguccucu     1440

gggcaccuca aaugcaggcu gaagauggac aagcugcggc ugaaaggagu gagcuauuca     1500

uuguguacug cugccuucac guuuacaaag auuccugcag agacccucca uggcacuguc     1560

accguggaag uucaguacgc ggguacagac gggccaugca aagugcccgc ucaaauggcc     1620

guagauaugc agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca     1800

auugguaagg ccuuugaagc uaccgucagg ggcgccaaac guauggcagu gcucggggac     1860

acagcuuggg auuucggaag uguaggcggu gcccugaaua gccuugggaa gggcauccac     1920

cagaucuucg gagcggccuu uaaauccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cucuuggggg aguguuaaua uuccugucaa cggcaguuag ugccgacguc     2100

ggcugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3899
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3899
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga gguuacgagg cgggguuccg cguacuauau guaccuggau      120

cgcaacgacg cuggcgaagc cauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga gugcccuaug      240

cuggaugaag gcguggagcc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaagccc gacguagcag aagggcugug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaguca      420

agagaguaca ccaagcaccu uauuaggguu gaaaauugga ucuuucggaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaggg gauguccgga gguacuuggg uugacauugu guuagaacau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaggugaga aguuacugcu augaagcuag cauaucugau      780

auggccuccg acucaaggug ucccacacaa ggagaggcgu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugaaaa ccucgaguac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugaaaccg acgagaacag agcuaaggug     1080

gagaucacac caaauucccc cagggccgaa gcuacucuug ggggauucgg cucacugggu     1140

uuggauugug agccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggaaugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaagagg cccuugugga auuuaaggac     1320

gcucacgcga aacgacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga ggcagaaaug gauggugcca agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaagauu ccugcagaga cccuccaugg cacugucacc     1560

guggaaguuc aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag caaacccagu gaucacggag     1680

aguaccgaaa acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggccu uugaagcuac cgucagaggc gccaaaagga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuc uugggggagu guuaauauuc cugucaacgg caguuagugc cgacgucggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3900
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3900
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaggu uacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

aacgacgcug gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug      240

gaugaaggcg uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caaaaagggg gaagcccgcc gaagccguag agcugugaca      360

cuaccguccc auucuaccag gaaacuccaa acgcggucgc agacuuggcu ggagucacgc      420

gaguacacca agcaccuuau uagaguugaa aauuggaucu uuaggaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc ggugcaucgg cgugagcaau      600

cgcgauuuug uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga ggugcgaagu uacugcuaug aagcuagcau aucugauaug      780

gccuccgacu caagaugucc cacacaagga gaggcguacc uugacaagca gagcgauacc      840

caguauguau gcaaaaggac uuugguggac cggggcuggg guaaugggug uggacuguuc      900

ggcaagggca gucuagucac augcgccaaa uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugaaaaccu cgaguaccgu aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gaaaccgacg agaaccgcgc uaagguggag     1080

aucacaccaa auucccccag agccgaagcu acucuugggg gauucggcuc acuggguuug     1140

gauugugagc cuaggaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aaacacuggc ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu     1320

cacgcgaaac ggcagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaggc agaaauggau ggugccaagg gccgacuguc cucugggcac     1440

cucaaaugcc gccugaagau ggacaagcug agacugaaag gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug     1560

gaaguucagu acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggcagguua auaacagcaa acccagugau cacggagagu     1680

accgaaaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcauc ggucuggauc aacaauuggu     1800

aaggccuuug aagcuaccgu ccgcggcgcc aaaagaaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc     1920

uucggagcgg ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcucuug ggggaguguu aauauuccug ucaacggcag uuagugccga cgucggcugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3901
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3901
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccuuacaacu       60

gcuauggcag ccgagguuac gagaagggga uccgcguacu auauguaccu ggaucggaac      120

gacgcuggug aagccauuuc uuuccccacc acauugggca ugaauaagug cuauauacag      180

aucauggauu uagggcacau gugugacgca acuaugucau acgagugccc uaugcuggau      240

gaaggagugg agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uauggcacuu gucaucacaa aaagggugaa gcccgccgaa gccguagagc ugugacacua      360

ccgucccauu cuaccaggaa acuccaaacg cggucgcaga cuuggcugga gucacgcgag      420

uacaccaagc accuuauuag aguugaaaau uggaucuuua ggaaccccgg guucgcacug      480

gccgcugccg cgauugcaug guugcucgga agcaguacau cccagaaagu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucggu gcaucggcgu gagcaaucgc      600

gauuuuguag agggcauguc cgggggaacu uggguugaca uuguguuaga acaugguggc      660

ugugucaccg ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaggu gcgaaguuac ugcuaugaag cuagcauauc ugauauggcc      780

uccgacucaa gaugucccac acaaggggag gcguaccuug acaagcagag cgauacccag      840

uauguaugca aaaggacuuu gguggaccgg ggauggggca augguugugg gcuguucgga      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaaaaugac cggcaagucc      960

auucagccug aaaaccucga guaccguauc augcugagcg ugcacggguc gcaacacucu     1020

ggaaugauug uuaaugauac gggucaugaa accgacgaga accgcgcuaa gguggagauc     1080

acaccaaauu cccccagagc cgaagcuacu cuuggcgggu ucggaucacu gggcuuggau     1140

ugugagccua ggaccggucu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gacaucccac ucccguggca cgccggggca     1260

gauacuggca caccccauug gaacaauaaa gaggcccuug uggaauuuaa ggacgcucac     1320

gcgaaacggc agaccguagu ugugcuggga agccaggagg gcgcagucca uaccgccuug     1380

gcuggggccc uagaggcaga aauggaugga gccaaggguc gacuguccuc uggccaccuc     1440

aaaugccgcc ugaagaugga caaacuuaga cugaaggggg ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gagacccucc auggaacugu caccguggaa     1560

guucaguacg cgggcacaga cgguccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg gagguuaaua acagcaaacc cagugaucac ggagaguacc     1680

gaaaacucca agaugaugcu ggagcuagau cccccguucg gcgacagcua cauuguuauc     1740

ggagugggcg agaaaaagau aacucaccac uggcaucggu cugggucaac aauuggaaag     1800

gccuuugaag cuaccguccg cggugccaaa agaauggcag ugcucggcga cacagcuugg     1860

gauuucggga guguaggagg cgcccugaau agccuuggua aggggaucca ccagaucuuc     1920

ggcgcggccu uuaaauccuu guucggaggc augucuuggu uuucgcaaau ucugaucggg     1980

acucuccuga uguggcuugg acugaacacc aagaauggca gcauuuccuu gaugugucuc     2040

gcucugggug ggguguuaau auuccuguca acggcaguua gugccgacgu cggaugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3902
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3902
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu uacaacugcc       60

auggcugcag agguuacgag aaggggaucc gccuacuaua uguaccugga ucggaacgac      120

gcgggugaag cuauuucuuu ccccaccaca uugggcauga auaagugcua uauacagauc      180

auggauuuag ggcacaugug ugacgccacu augucauacg agugcccuau gcuggaugaa      240

ggaguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

ggcacuuguc aucacaaaaa gggugaagca cgccgaagcc guagagccgu gacacuaccg      360

ucccauucua ccaggaaacu ccaaacgcgg ucgcagacuu ggcuggaguc acgcgaguac      420

accaagcacc uuauuagagu ugaaaauugg aucuuuagga accccggguu cgcucuggca      480

gccgcugcca uugcgugguu gcucggaagc aguacauccc agaaagugau cuaucugguc      540

augauacugc ucaucgcacc cgccuacucu auucggugca ucggcgugag caaucgcgau      600

uuuguagagg gcauguccgg gggaacuugg guugacauug uguuagaaca ugguggcugu      660

gucaccguga uggcucagga uaagccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg ccgaggugcg aaguuacugc uaugaagcaa gcauaucuga uauggcuucc      780

gacucaagau gucccacaca aggggaggcc uaccuugaca agcagagcga uacccaguau      840

guaugcaaaa ggacuuuggu ggaccgggga uggggcaaug guugugggcu guucggaaag      900

ggcagucuag ucacaugcgc gaaauuugcc ugcucuaaga aaaugaccgg caaguccauu      960

cagccugaaa accucgagua ccguaucaug cugagcgugc acgggucgca acacucugga     1020

augauuguua augauacggg ucaugaaacc gacgagaacc gcgcaaaggu ggagaucaca     1080

ccaaauuccc ccagagcuga agccacucuu ggcggguucg gaucacuggg cuuggauugu     1140

gagccuagga ccggucucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc uggggccgau     1260

acuggcaccc cucauuggaa caauaaagag gcacuugugg aauuuaagga cgcccacgcu     1320

aaacggcaga caguaguugu gcugggaagc caggagggcg cgguccauac cgcauuggcc     1380

ggggcucuag aggccgaaau ggauggagca aagggucgac uguccucugg ccaccucaaa     1440

ugccgccuga agauggacaa acuuagacug aaggggguga gcuauucauu guguacugcc     1500

gcuuucacgu uuacaaagau uccugccgag acccuccaug gaacugucac cguggaaguu     1560

caguacgcag gcacagacgg uccaugcaaa gugcccgcgc aaauggcugu agauaugcag     1620

acccugacuc cugucgggag guuaauaaca gccaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggcg acagcuacau uguuaucgga     1740

gugggcgaga aaaagauaac ucaccacugg caucggucug ggucaacaau uggaaaggca     1800

uuugaagcca ccguccgcgg ugcuaaaaga auggccgugc ucggcgacac agcaugggau     1860

uucgggagug uaggaggcgc ucugaauagc cuugguaagg ggauccacca gaucuucggc     1920

gccgcguuua aauccuuguu cggaggcaug ucuugguuuu cgcaaauucu gaucgggacu     1980

cuccugaugu ggcuuggacu gaacaccaag aauggcagca uuuccuugau gugucucgcc     2040

cugggugggg uguuaauauu ccugucaacg gcuguuagug cagacgucgg augcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3903
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3903
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcag augucggaug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3904
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3904
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagccg uuacgagaag gggauccgcg uacuauaugu accuggaccg gaacgaugcu      120

ggugaggcca uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcaacuaug ucauacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaggcccgc cgaagccgua gagcugugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggaaucacg cgaguacacc      420

aagcaccuua uuagaguuga gaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagaaggca uguccggggg aacuuggguu gauauugugu uagagcaugg uggcuguguc      660

accgugaugg cccaggacaa gccaacagug gauaucgaac uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaggcuagca uaucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaagcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugagaacc ucgaauaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugagaccgau gaaaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaggc uacucuuggc ggguucggau cacugggcuu ggacugugaa     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggagugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacacu     1260

ggcacacccc auuggaacaa uaaagaagcc cuuguggagu uuaaggaugc ucacgcgaaa     1320

cggcagaccg uaguugugcu gggaagccag gaaggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagagaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagaaacc cuccauggaa cugucaccgu ggagguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaggcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccg augucggaug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3905
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3905
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccgc gguuacgagg cgggguuccg cuuacuauau guaccuggau      120

cgcaacgacg ccggcgaggc aauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgccacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaggcuc gacguagcag aagggcagug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaauca      420

agagaguaca ccaagcaccu uauuaggguu gagaauugga ucuuucggaa ccccggauuc      480

gcccuggcug ccgcggcaau ugccugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgcuccu gccuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcacaggau aagccaacag uggacaucga acuggucacc      720

acgacuguuu caaacauggc ugaggugaga aguuacugcu augaggccag cauaucugau      780

auggcguccg acucaaggug ucccacacaa ggagaagccu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgca aaauuugcuu gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgaaaacag agccaaggug     1080

gagaucacac caaauucccc cagggcugag gccacucuug ggggauucgg cucacugggu     1140

uuggauugug aaccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgca     1260

ggcgccgaua cuggaacacc ccauuggaac aauaaagaag cucuugugga guuuaaggac     1320

gcgcacgcaa aacgacagac cguaguugug cugggcagcc aggaaggggc cguccauacc     1380

gcuuuggccg gagcacuaga ggccgagaug gauggugcua agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugccg cauucacguu uacaaagauu ccugcggaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcugg uacagacggg ccaugcaaag ugcccgccca aauggcagua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag ccaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggaa cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggcuu uugaggccac cgucagaggc gcaaaaagga uggcugugcu cggggacaca     1860

gccugggauu ucggaagugu aggcggugcg cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag ccgcuuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcac uugggggagu guuaauauuc cugucaacgg ccguuagugc ugacgucggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3906
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3906
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagguguuac ucgacguggc      120

ucagccuacu auauguaccu ggauagaaac gacgcugggg aggcaauuag uuuucccacg      180

acccuaggaa ugaauaaaug cuauauacaa aucauggauc ucggccauau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggugugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugggaccu gucaccauaa gaaaggagag      360

gcgaggcggu cucgcagagc ugugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg cuuugcccug gcagccgcug caauugccug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc uccugccuac      600

uccauuaggu gcaucggggu gucaaaucgg gauuucguag aaggaaugag ugguggcaca      660

uggguugaca uuguguugga gcacggggga ugugucacug ugauggcgca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggcagaggu gcguucuuac      780

ugcuaugagg ccuccauauc agauauggcu agcgacaguc gcugucccac ucagggcgaa      840

gccuaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

gguuggggga auggaugugg ccuguuuggc aaaggguccc uugucacuug cgcaaaguuc      960

gcuugcagca aaaagaugac aggaaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacgguuc ccagcauuca ggcaugauug uuaaugauac cgggcacgag     1080

acggacgaaa accgagccaa aguggagauc accccaaaua guccccgcgc ggaggccaca     1140

cucggaggcu uugguagccu gggguuagau ugugaaccua gaacuggccu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcaggagcu gauacaggca cuccccacug gaacaauaag     1320

gaagcccuag uggaguucaa agacgcucau gccaagaggc agaccguagu ugugcugggg     1380

ucucaggaag gagcagucca cacagcccuc gcuggugcgc uggaggcaga gauggauggc     1440

gccaaagggc ggcuuagcuc aggacaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggcg ugaguuauuc cuuauguacc gcugccuuua cuuucacgaa aauuccugca     1560

gaaacacugc acgguaccgu cacuguggag guucaauacg ccgggaccga cggcccaugc     1620

aaggugcccg cucagauggc cguagauaug cagacacuaa ccccugucgg aaggcucaua     1680

acugcaaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gcgacucaua cauuguuauc ggggugggag agaagaagau aacccaccau     1800

uggcaccgua gugguagcac uauuggcaaa gcguucgagg cuacaguccg aggggccaag     1860

cggauggcag uguugggaga caccgccugg gauuucggcu ccguaggugg ggcucucaau     1920

ucucugggca aaggaaucca ucaaaucuuu ggcgccgcau ucaagucgcu guuuggggga     1980

augagcuggu ucucccagau ucuuaucggc acacuguuga uguggcucgg ucugaacacu     2040

aaaaaugggu caauuaguuu aaugugucug gcucuaggag gcgugcucau auuucugucu     2100

accgccguua gcgcggacgu cgguugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3907
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3907
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagccguuac ucgacguggu      120

ucagcuuacu auauguaccu ggauagaaac gacgcaggcg aggccauuag uuuucccacg      180

acccuaggga ugaauaaaug cuauauacaa aucauggauc ucggacauau gugugacgcg      240

acaaugagcu acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugguaccu gucaccauaa gaaaggggag      360

gcuaggcggu cucgcagagc cgugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg auuugcacug gccgcugcag ccauugcuug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc cccugcguac      600

uccauuaggu gcaucggcgu gucaaaucgg gauuucguag aagggaugag uggagguaca      660

uggguugaca uuguguugga gcacggcggg ugugucacug ugauggcaca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggccgaggu gcguucuuac      780

ugcuaugagg cuuccauauc agauauggcc agcgacaguc gcugucccac ucagggagaa      840

gcauaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

ggcuggggua augggugugg acuguuuggc aaaggcuccc uugucacuug cgcuaaguuc      960

gccugcagca aaaagaugac agggaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacggauc ccagcauuca gguaugauug uuaaugauac cggccacgag     1080

acggacgaaa accgagcgaa aguggagauc accccaaaua guccccgcgc cgaggcaaca     1140

cucgggggau uuggcagccu ggguuuagau ugugaaccua gaacugggcu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcuggcgcc gauacaggaa cuccccacug gaacaauaag     1320

gaagcucuag uggaguucaa agacgcccau gcaaagaggc agaccguagu ugugcugggc     1380

ucucaggaag gggccgucca cacagcucuc gcgggagcac uggaggccga gauggauggu     1440

gcuaaaggcc ggcuuagcuc agggcaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggag ugaguuauuc cuuauguacc gccgcauuua cuuucacgaa aauuccugcc     1560

gaaacacugc acggcaccgu cacuguggag guucaauacg cugguaccga cgggccaugc     1620

aaggugcccg cccagauggc aguagauaug cagacacuaa ccccugucgg caggcucaua     1680

acugcgaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gagacucaua cauuguuauc ggcguggggg agaagaagau aacccaccau     1800

uggcaccgua guggaagcac uauugguaag gcuuucgagg ccacaguccg gggcgcaaaa     1860

cgaauggccg uguuggggga caccgcuugg gauuucggau ccguaggcgg ugcccucaau     1920

ucucugggga aaggcaucca ucaaaucuuu ggagcagcuu ucaagucgcu guuuggcggg     1980

augagcuggu ucucccagau ucuuaucgga acacuguuga uguggcucgg ccugaacacu     2040

aaaaaugguu caauuaguuu aaugugucug gcccuagggg gagugcucau auuucugucu     2100

accgcgguua gcgccgacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3908
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3908
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  3909
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3909
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagaggcg gggcgcugac       60

accagcgugg ggauagucgg acugcuccuu acaacugcaa uggccgcgga aguuacgcgc      120

cgagguuccg cuuacuauau guaccuggau cguaaugacg ccggcgaggc aaucucuuuc      180

cccaccacau uggggaugaa caaaugcuau auucagauca uggauuuagg acacaugugu      240

gacgccacua ugucauacga augcccuaug cuggaugagg gcguggaacc agacgaugua      300

gacuguuggu gcaauaccac aaguaccugg guggucuaug guacuuguca ucacaagaaa      360

ggggaggcua gaaggagccg gcgcgcagug acacuaccgu cccauucuac cagaaagcuc      420

caaacgaggu cgcagacuug gcuggaguca cgggaauaca ccaagcaccu uauucgcguu      480

gagaacugga ucuuucgaaa ucccggauuc gcccuggcug ccgcggcaau agccugguug      540

cucggcagca guacauccca gaaagugauc uaucugguca ugauucugcu caucgcuccu      600

gccuacucua uuagaugcau cggcgugagc aacagggauu uuguagaagg gauguccgga      660

gguacuuggg uugacauagu guuagagcau ggcgggugug ucaccgugau ggcacaggau      720

aagccaacag uggacauuga gcuggucacc acgacuguuu caaauauggc ugaagugcgg      780

aguuacugcu augaggccag caucucugau auggcguccg acucacguug ucccacacaa      840

ggagaagccu accuugacaa acagagcgau acccaguaug uaugcaagcg cacuuuggug      900

gacagaggcu gggguaacgg guguggacug uucggcaaag gcagucuagu cacaugcgca      960

aaguuugcuu gcucuaagaa aaugaccggg aaguccauuc agccugagaa ucucgaauac     1020

aggaucaugc ugagcgugca cggaucgcaa cacucuggua ugaucguuaa cgauacgggc     1080

caugagaccg acgagaaucg ggccaaagug gaaauuacac caaacucacc ccgagcugag     1140

gccacucuug ggggauucgg cucccugggu uuggauugug aaccucgcac cgggcucgac     1200

uuuagugauc uguauuacuu aacaaugaau aacaagcacu ggcuggucca uaaagagugg     1260

uuccacgaca uaccacuccc guggcacgca ggcgccgaua cuggaacacc ccauuggaau     1320

aacaaggaag cucuugugga guuuaaagac gcgcacgcaa agagacagac cguaguugug     1380

cugggcagcc aggagggggc cguccauacc gcuuuggccg gagcacuaga agccgagaug     1440

gauggugcua agggcaggcu guccucuggg caccucaaau gccggcugaa gauggacaaa     1500

cuucgccuga agggagugag cuauucauug uguacugccg cauucacguu uacaaaaauc     1560

ccugcggaaa cccuccaugg cacugucacc guggagguuc aguacgcugg uacagacggg     1620

ccaugcaagg ugcccgccca aauggcagua gauaugcaga cccugacucc ugucggcaga     1680

uuaauuacag ccaacccagu gaucacggaa aguaccgaaa auuccaagau gaugcuggag     1740

cuagaucccc cguucggaga cagcuacaua guuauuggcg ugggggagaa aaagaucacu     1800

caccacuggc auaggucugg aucaacaauc gguaaggcuu uugaggccac cguccguggc     1860

gcaaaacgga uggcugugcu cggggacaca gccugggauu ucggaagugu aggcggugcg     1920

cugaacagcc uugggaaagg cauucaccag aucuucggag ccgcuuuuaa guccuuguuc     1980

ggcgggaugu cuugguuuuc gcaaauucug auaggaacuc uccugaugug gcuuggccug     2040

aauaccaaga acgguagcau cuccuugaug ugucucgcau uagggggagu gcugauuuuc     2100

cugucaacgg ccguuagugc ugacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3910
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3910
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agaggcgggg cgcugacacc       60

agcgugggga uugucggacu gcuccuuaca acugcaaugg ccgcggaagu uacgcgccga      120

gguuccgcuu acuauaugua ccuggaucgu aaugacgccg gcgaggcaau aucuuucccc      180

accacauugg ggaugaacaa augcuauauc cagauuaugg auuuaggaca caugugugac      240

gccacuaugu cauacgaaug cccuaugcug gaugagggcg uggaaccaga cgauguagac      300

uguuggugca auaccacaag uaccugggug gucuauggua cuugucauca caagaaaggg      360

gaggcuagaa ggagccggcg cgcagugaca cuaccguccc auucuaccag aaagcuccaa      420

acgaggucgc agacuuggcu ggagucacgg gaauacacca agcaccuuau ccgcguugag      480

aacuggauuu uucgaaaucc cggauucgcc cuggcugccg cggcaaucgc cugguugcuc      540

ggcagcagua caucccagaa agugauauau cuggucauga uccugcucau ugcuccugcc      600

uacucuauca gaugcauugg cgugagcaac agggauuuug uagaagggau guccggaggu      660

acuuggguug acaucguguu agagcauggc ggguguguca ccgugauggc acaggauaag      720

ccaacagugg acauagagcu ggucaccacg acuguuucaa auauggcuga agugcggagu      780

uacugcuaug aggccagcau uucugauaug gcguccgacu cacguugucc cacacaagga      840

gaagccuacc uugacaaaca gagcgauacc caguauguau gcaagcgcac uuugguggac      900

agaggcuggg guaacgggug uggacuguuc ggcaaaggca gucuagucac augcgcaaag      960

uuugcuugcu cuaagaaaau gaccgggaag uccauccagc cugagaaucu cgaauacagg     1020

auuaugcuga gcgugcacgg aucgcaacac ucugguauga ucguuaacga uacgggccau     1080

gagaccgacg agaaucgggc caaaguggaa aucacaccaa acucaccccg agcugaggcc     1140

acucuugggg gauucggcuc ccuggguuug gauugugaac cucgcaccgg gcucgacuuu     1200

agugaucugu auuacuuaac aaugaauaac aagcacuggc ugguccauaa agagugguuc     1260

cacgacauuc cacucccgug gcacgcaggc gccgauacug gaacacccca uuggaauaac     1320

aaggaagcuc uuguggaguu uaaagacgcg cacgcaaaga gacagaccgu aguugugcug     1380

ggcagccagg agggggccgu ccauaccgcu uuggccggag cacuagaagc cgagauggau     1440

ggugcuaagg gcaggcuguc cucugggcac cucaaaugcc ggcugaagau ggacaaacuu     1500

cgccugaagg gagugagcua uucauugugu acugccgcau ucacguuuac aaaaauaccu     1560

gcggaaaccc uccauggcac ugucaccgug gagguucagu acgcugguac agacgggcca     1620

ugcaaggugc ccgcccaaau ggcaguagau augcagaccc ugacuccugu cggcagauua     1680

aucacagcca acccagugau uacggaaagu accgaaaauu ccaagaugau gcuggagcua     1740

gaucccccgu ucggagacag cuacaucguu auaggcgugg gggagaaaaa gauuacucac     1800

cacuggcaua ggucuggauc aacaaucggu aaggcuuuug aggccaccgu ccguggcgca     1860

aaacggaugg cugugcucgg ggacacagcc ugggauuucg gaaguguagg cggugcgcug     1920

aacagccuug ggaaaggcau ccaccagauu uucggagccg cuuuuaaguc cuuguucggc     1980

gggaugucuu gguuuucgca aauccugauu ggaacucucc ugauguggcu uggccugaau     2040

accaagaacg guagcauauc cuugaugugu cucgcauuag ggggagugcu gaucuuccug     2100

ucaacggccg uuagugcuga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3911
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3911
gggagaaagc uuaccaugaa cgccagaaag gagaaaaaga ggcggggcgc ugacaccagc       60

guggggaucg ucggacugcu ccuuacaacu gcaauggccg cggaaguuac gcgccgaggu      120

uccgcuuacu auauguaccu ggaucguaau gacgccggcg aggcaauuuc uuuccccacc      180

acauugggga ugaacaaaug cuauauacag aucauggauu uaggacacau gugugacgcc      240

acuaugucau acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaua ccacaaguac cugggugguc uaugguacuu gucaucacaa gaaaggggag      360

gcuagaagga gccggcgcgc agugacacua ccgucccauu cuaccagaaa gcuccaaacg      420

aggucgcaga cuuggcugga gucacgggaa uacaccaagc accuuauucg cguugagaac      480

uggaucuuuc gaaaucccgg auucgcccug gcugccgcgg caauugccug guugcucggc      540

agcaguacau cccagaaagu gaucuaucug gucaugauac ugcucaucgc uccugccuac      600

ucuauuagau gcaucggcgu gagcaacagg gauuuuguag aagggauguc cggagguacu      660

uggguugaca uuguguuaga gcauggcggg ugugucaccg ugauggcaca ggauaagcca      720

acaguggaca ucgagcuggu caccacgacu guuucaaaua uggcugaagu gcggaguuac      780

ugcuaugagg ccagcauauc ugauauggcg uccgacucac guugucccac acaaggagaa      840

gccuaccuug acaaacagag cgauacccag uauguaugca agcgcacuuu gguggacaga      900

ggcuggggua acgggugugg acuguucggc aaaggcaguc uagucacaug cgcaaaguuu      960

gcuugcucua agaaaaugac cgggaagucc auucagccug agaaucucga auacaggauc     1020

augcugagcg ugcacggauc gcaacacucu gguaugauug uuaacgauac gggccaugag     1080

accgacgaga aucgggccaa aguggaaauc acaccaaacu caccccgagc ugaggccacu     1140

cuugggggau ucggcucccu ggguuuggau ugugaaccuc gcaccgggcu cgacuuuagu     1200

gaucuguauu acuuaacaau gaauaacaag cacuggcugg uccauaaaga gugguuccac     1260

gacaucccac ucccguggca cgcaggcgcc gauacuggaa caccccauug gaauaacaag     1320

gaagcucuug uggaguuuaa agacgcgcac gcaaagagac agaccguagu ugugcugggc     1380

agccaggagg gggccgucca uaccgcuuug gccggagcac uagaagccga gauggauggu     1440

gcuaagggca ggcuguccuc ugggcaccuc aaaugccggc ugaagaugga caaacuucgc     1500

cugaagggag ugagcuauuc auuguguacu gccgcauuca cguuuacaaa aauuccugcg     1560

gaaacccucc auggcacugu caccguggag guucaguacg cugguacaga cgggccaugc     1620

aaggugcccg cccaaauggc aguagauaug cagacccuga cuccugucgg cagauuaaua     1680

acagccaacc cagugaucac ggaaaguacc gaaaauucca agaugaugcu ggagcuagau     1740

cccccguucg gagacagcua cauuguuauc ggcguggggg agaaaaagau aacucaccac     1800

uggcauaggu cuggaucaac aauugguaag gcuuuugagg ccaccguccg uggcgcaaaa     1860

cggauggcug ugcucgggga cacagccugg gauuucggaa guguaggcgg ugcgcugaac     1920

agccuuggga aaggcaucca ccagaucuuc ggagccgcuu uuaaguccuu guucggcggg     1980

augucuuggu uuucgcaaau ucugaucgga acucuccuga uguggcuugg ccugaauacc     2040

aagaacggua gcauuuccuu gaugugucuc gcauuagggg gagugcugau auuccuguca     2100

acggccguua gugcugacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3912
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3912
gggagaaagc uuaccauggc cagaaaggag aaaaagaggc ggggcgcuga caccagcgug       60

gggaucgucg gacugcuccu uacaacugca auggccgcgg aaguuacgcg ccgagguucc      120

gcuuacuaua uguaccugga ucguaacgac gccggcgagg caauuucuuu ccccaccaca      180

uuggggauga auaaaugcua uauacagauc auggauuuag gacacaugug ugacgccacu      240

augucauacg aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg      300

ugcaacacca caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu      360

agaaggagcc ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccaaacgagg      420

ucgcagacuu ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaauugg      480

aucuuucgaa accccggauu cgcccuggcu gccgcggcaa uugccugguu gcucggcagc      540

aguacauccc agaaagugau cuaucugguc augauacugc ucaucgcucc ugccuacucu      600

auuagaugca ucggcgugag caauagggau uuuguagaag ggauguccgg agguacuugg      660

guugacauug uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca      720

guggacaucg agcuggucac cacgacuguu ucaaacaugg cugaagugcg gaguuacugc      780

uaugaggcca gcauaucuga uauggcgucc gacucacguu gucccacaca aggagaagcc      840

uaccuugaca aacagagcga uacccaguau guaugcaagc gcacuuuggu ggacagaggc      900

ugggguaaug gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu      960

ugcucuaaga aaaugaccgg gaaguccauu cagccugaga accucgaaua caggaucaug     1020

cugagcgugc acggaucgca acacucuggu augauuguua augauacggg ccaugagacc     1080

gacgagaacc gggccaaagu ggaaaucaca ccaaauucac cccgagcuga ggccacucuu     1140

gggggauucg gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau     1200

cuguauuacu uaacaaugaa caauaagcac uggcuggucc auaaagagug guuccacgac     1260

aucccacucc cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa     1320

gcucuugugg aguuuaaaga cgcgcacgca aagagacaga ccguaguugu gcugggcagc     1380

caggaggggg ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu     1440

aagggcaggc uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcauucacgu uuacaaaaau uccugcggaa     1560

acccuccaug gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag     1620

gugcccgccc aaauggcagu agauaugcag acccugacuc cugucggcag auuaauaaca     1680

gccaacccag ugaucacgga aaguaccgag aacuccaaga ugaugcugga gcuagauccc     1740

ccguucggag acagcuacau uguuaucggc gugggggaga aaaagauaac ucaccacugg     1800

cauaggucug gaucaacaau ugguaaggcu uuugaagcca ccguccgugg cgcaaaacgg     1860

auggcugugc ucggggacac agccugggau uucggaagug uaggcggugc gcugaauagc     1920

cuugggaaag gcauccacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug     1980

ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag     2040

aaugguagca uuuccuugau gugucucgca uuagggggag ugcugauauu ccugucaacg     2100

gccguuagug cugacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3913
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3913
gggagaaagc uuaccaugag aaaggagaaa aagaggcggg gcgccgacac cagcgugggg       60

aucgucggac ugcuccuuac aacugcuaug gcagccgaag uuacgcgccg agguuccgcg      120

uacuauaugu accuggaucg uaacgacgcu ggcgaggcca uuucuuuccc caccacauug      180

gggaugaaua aaugcuauau acagaucaug gauuuaggac acauguguga cgcaacuaug      240

ucauacgaau gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc      300

aacaccacaa guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggccaga      360

aggagccggc gcgcugugac acuaccgucc cauucuacca gaaagcucca aacgaggucg      420

cagacuuggc uggagucacg ggaauacacc aagcaccuua uucgcguuga gaauuggauc      480

uuucgaaacc ccggauucgc acuggccgcu gccgcgauug caugguugcu cggcagcagu      540

acaucccaga aagugaucua ucuggucaug auacugcuca ucgccccugc uuacucuauu      600

agaugcaucg gcgugagcaa uagggauuuu guagaaggga uguccggagg uacuuggguu      660

gacauugugu uagagcaugg cggguguguc accgugaugg cccaggauaa gccaacagug      720

gacaucgagc uggucaccac gacuguuuca aacauggcag aagugcggag uuacugcuau      780

gaggcuagca uaucugauau ggccuccgac ucacguuguc ccacacaagg agaagcguac      840

cuugacaaac agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg      900

gguaaugggu guggacuguu cggcaaaggc agucuaguca caugcgccaa guuugcaugc      960

ucuaagaaaa ugaccgggaa guccauucag ccugagaacc ucgaauacag gaucaugcug     1020

agcgugcacg gaucgcaaca cucugguaug auuguuaaug auacgggcca ugagaccgac     1080

gagaaccggg cuaaagugga aaucacacca aauucacccc gagccgaggc uacucuuggg     1140

ggauucggcu cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug     1200

uauuacuuaa caaugaacaa uaagcacugg cugguccaua aagagugguu ccacgacauc     1260

ccacucccgu ggcacgccgg cgcagauacu ggaacacccc auuggaacaa uaaggaagcc     1320

cuuguggagu uuaaagacgc ucacgcgaag agacagaccg uaguugugcu gggcagccag     1380

gagggggcag uccauaccgc cuuggcugga gcccuagaag cagagaugga uggugccaag     1440

ggcaggcugu ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag     1500

ggagugagcu auucauugug uacugcugcc uucacguuua caaaaauucc ugcagaaacc     1560

cuccauggca cugucaccgu ggagguucag uacgcgggua cagacgggcc augcaaggug     1620

cccgcucaaa uggccguaga uaugcagacc cugacuccug ucggcagauu aauaacagca     1680

aacccaguga ucacggaaag uaccgagaac uccaagauga ugcuggagcu agaucccccg     1740

uucggagaca gcuacauugu uaucggcgug ggggagaaaa agauaacuca ccacuggcau     1800

aggucuggau caacaauugg uaaagccuuu gaagcuaccg uccguggcgc caagcggaug     1860

gcagugcucg gggacacagc uugggauuuc ggaaguguag gcggugcccu gaauagccuu     1920

gggaaaggca uccaccagau cuucggagcg gccuuuaagu ccuuguucgg cgggaugucu     1980

ugguuuucgc aaauucugau cggaacucuc cugauguggc uuggccugaa caccaagaau     2040

gguagcauuu ccuugaugug ucucgcuuua gggggagugc ugauauuccu gucaacggca     2100

guuagugccg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3914
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3914
gggagaaagc uuaccaugaa ggagaaaaag agaaggggcg ccgacaccag cguggggauc       60

gucggacugc uccuuacaac ugcuauggca gccgaaguua cgcggcgcgg uuccgcguac      120

uauauguacc uggaucgaaa cgacgcuggc gaggccauuu cuuuccccac cacauugggg      180

augaauaaau gcuauauaca gaucauggau uuaggacaca ugugugacgc aacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcccguaga      360

agcaggcggg cugugacacu accgucccau ucuacccgca agcuccaaac gagaucgcag      420

acuuggcugg agucaaggga auacaccaag caccuuauuc ggguugagaa uuggaucuuu      480

cgcaaccccg gauucgcacu ggccgcugcc gcgauugcau gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugaua cugcucaucg ccccugcuua cucuauucga      600

ugcaucggcg ugagcaauag agauuuugua gaagggaugu ccggagguac uuggguugac      660

auuguguuag agcauggcgg gugugucacc gugauggccc aggauaagcc aacaguggac      720

aucgagcugg ucaccacgac uguuucaaac auggcagaag ugaggaguua cugcuaugag      780

gcuagcauau cugauauggc cuccgacuca cgguguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guauguaugc aagcguacuu ugguggaccg cggcuggggu      900

aaugggugug gacuguucgg caaaggcagu cuagucacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaccucg aauacagaau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauu guuaaugaua cgggccauga gaccgacgag     1080

aacagggcua aaguggaaau cacaccaaau ucaccccggg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagcgc cagaccguag uugugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

agacuguccu cugggcaccu caaaugcagg cugaagaugg acaaacuucg gcugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauuccugc agaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gccgcuuaau aacagcaaac     1680

ccagugauca cggaaaguac cgagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauuguuau cggcgugggg gagaaaaaga uaacucacca cuggcauaga     1800

ucuggaucaa caauugguaa agccuuugaa gcuaccguca ggggcgccaa gcguauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

agcauuuccu ugaugugucu cgcuuuaggg ggagugcuga uauuccuguc aacggcaguu     2100

agugccgacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3915
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3915
gggagaaagc uuaccaugga gaagaaaaga aggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc uuacaacugc uauggcagcc gaaguuacgc ggcgcgguuc cgcguacuau      120

auguaccugg aucgaaacga cgcuggcgag gccauuucuu uccccaccac auuggggaug      180

aauaagugcu auauacagau cauggauuua ggacacaugu gugacgcaac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaaa agggggaggc ccguagaagc      360

aggcgggcug ugacacuacc gucccauucu acccgcaaac uccaaacgag aucgcagacu      420

uggcuggagu caagggaaua caccaagcac cuuauucggg uugagaauug gaucuuucgc      480

aaccccggau ucgcacuggc cgcugccgcg auugcauggu ugcucggcag caguacaucc      540

cagaagguga ucuaucuggu caugauacug cucaucgccc cugcuuacuc uauucgaugc      600

aucggcguga gcaauagaga uuuuguagaa gggauguccg gagguacuug gguugacauu      660

guguuagagc auggcgggug ugucaccgug auggcccagg auaaaccaac aguggacauc      720

gagcugguca ccacgacugu uucaaacaug gcagaaguga ggaguuacug cuaugaggcu      780

agcauaucug auauggccuc cgacucacgg ugucccacac aaggagaagc guaccuugac      840

aagcagagcg auacccagua uguaugcaaa cguacuuugg uggaccgcgg cugggguaau      900

ggguguggac uguucggcaa gggcagucua gucacaugcg ccaaauuugc augcucuaag      960

aagaugaccg ggaaauccau ucagccugag aaccucgaau acagaaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugauuguu aaugauacgg gccaugagac cgacgagaac     1080

agggcuaagg uggaaaucac accaaauuca ccccgggccg aggcuacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucga accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga acaauaaaca cuggcugguc cauaaggagu gguuccacga caucccacuc     1260

ccguggcacg ccggcgcaga uacuggaaca ccccauugga acaauaaaga agcccuugug     1320

gaguuuaagg acgcucacgc gaaacgccag accguaguug ugcugggcag ccaggagggg     1380

gcaguccaua ccgccuuggc uggagcccua gaagcagaga uggauggugc caagggcaga     1440

cuguccucug ggcaccucaa gugcaggcug aaaauggaca agcugcggcu gaaaggagug     1500

agcuauucau uguguacugc ugccuucacg uuuacaaaga uuccugcaga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcg gguacagacg ggccaugcaa agugcccgcu     1620

caaauggccg uagauaugca gacccugacu ccugucggcc gcuuaauaac agcaaaccca     1680

gugaucacgg aaaguaccga gaacuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uuguuaucgg cgugggggaa aagaaaauaa cucaccacug gcauagaucu     1800

ggaucaacaa uugguaaggc cuuugaggcu accgucaggg gcgccaaacg uauggcagug     1860

cucggggaca cagcuuggga uuucggaagu guaggcggug cccugaauag ccuugggaag     1920

ggcauccacc agaucuucgg agcggccuuu aaauccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugaucggaac ucuccugaug uggcuuggcc ugaacaccaa gaaugguagc     2040

auuuccuuga ugugucucgc ucuuggggga guguuaauau uccugucaac ggcaguuagu     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3916
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3916
gggagaaagc uuaccaugaa gaaaagaagg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuua caacugcuau ggcagccgag guuacgcggc gcgguuccgc guacuauaug      120

uaccuggauc gaaacgacgc uggcgaagcc auuucuuucc ccaccacauu ggggaugaau      180

aagugcuaua uacagaucau ggauuuagga cacaugugug acgcaacuau gucauacgag      240

ugcccuaugc uggaugaagg cguggagcca gacgauguag acuguuggug caacaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaaaaagg gggaagcccg uagaagcagg      360

cgggcuguga cacuaccguc ccauucuacc cgcaaacucc aaacgagauc gcagacuugg      420

cuggagucaa gggaguacac caagcaccuu auucggguug aaaauuggau cuuucgcaac      480

cccggauucg cacuggccgc ugccgcgauu gcaugguugc ucggcagcag uacaucccag      540

aaggugaucu aucuggucau gauacugcuc aucgccccug cuuacucuau ucgaugcauc      600

ggcgugagca auagagauuu uguagagggg auguccggag guacuugggu ugacauugug      660

uuagaacaug gcgggugugu caccgugaug gcccaggaua aaccaacagu ggacaucgag      720

cuggucacca cgacuguuuc aaacauggca gaggugagga guuacugcua ugaagcuagc      780

auaucugaua uggccuccga cucacggugu cccacacaag gagaggcgua ccuugacaag      840

cagagcgaua cccaguaugu augcaaacgu acuuuggugg accgcggcug ggguaauggg      900

uguggacugu ucggcaaggg cagucuaguc acaugcgcca aauuugcaug cucuaagaag      960

augaccggga aauccauuca gccugaaaac cucgaguaca gaaucaugcu gagcgugcac     1020

ggaucgcaac acucugguau gauuguuaau gauacgggcc augaaaccga cgagaacagg     1080

gcuaaggugg agaucacacc aaauucaccc cgggccgaag cuacucuugg gggauucggc     1140

ucccuggguu uggauuguga gccucgaacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgacau cccacucccg     1260

uggcacgccg gcgcagauac uggaacaccc cauuggaaca auaaagaggc ccuuguggaa     1320

uuuaaggacg cucacgcgaa acgccagacc guaguugugc ugggcagcca ggagggggca     1380

guccauaccg ccuuggcugg agcccuagag gcagaaaugg auggugccaa gggcagacug     1440

uccucugggc accucaagug caggcugaaa auggacaagc ugcggcugaa aggagugagc     1500

uauucauugu guacugcugc cuucacguuu acaaagauuc cugcagagac ccuccauggc     1560

acugucaccg uggaaguuca guacgcgggu acagacgggc caugcaaagu gcccgcucaa     1620

auggccguag auaugcagac ccugacuccu gucggccgcu uaauaacagc aaacccagug     1680

aucacggaga guaccgaaaa cuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacauug uuaucggcgu gggggagaag aaaauaacuc accacuggca uagaucugga     1800

ucaacaauug guaaggccuu ugaagcuacc gucaggggcg ccaaacguau ggcagugcuc     1860

ggggacacag cuugggauuu cggaagugua ggcggugccc ugaauagccu ugggaagggc     1920

auccaccaga ucuucggagc ggccuuuaaa uccuuguucg gcgggauguc uugguuuucg     1980

caaauucuga ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu     2040

uccuugaugu gucucgcucu ugggggagug uuaauauucc ugucaacggc aguuagugcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3917
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3917
gggagaaagc uuaccaugaa gagaaggggc gccgacacca gcguggggau cgucggacug       60

cuccuuacaa cugcuauggc agccgagguu acgcggcgcg guuccgcgua cuauauguac      120

cuggaucgaa acgacgcugg cgaagccauu ucuuucccca ccacauuggg gaugaauaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg caacuauguc auacgagugc      240

ccuaugcugg augaaggcgu ggagccagac gauguagacu guuggugcaa caccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aagcccguag aagcaggcgg      360

gcugugacac uaccguccca uucuacccgc aagcuccaaa cgagaucgca gacuuggcug      420

gagucaaggg aguacaccaa acaccuuauu cggguugaaa auuggaucuu ucgcaacccc      480

ggauucgcac uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaag      540

gugaucuauc uggucaugau acugcucauc gccccugcuu acucuauucg augcaucggc      600

gugagcaaua gagauuuugu agaggggaug uccggaggua cuuggguuga cauuguguua      660

gaacauggcg ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa cauggcagag gugaggaguu acugcuauga agcuagcaua      780

ucugauaugg ccuccgacuc acgguguccc acacaaggag aggcguaccu ugacaaacag      840

agcgauaccc aguauguaug caagcguacu uugguggacc gcggcugggg uaaugggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaaaaagaug      960

accgggaagu ccauucagcc ugaaaaccuc gaguacagaa ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaaugau acgggccaug aaaccgacga gaacagggcu     1080

aaaguggaga ucacaccaaa uucaccccgg gccgaagcua cucuuggggg auucggcucc     1140

cuggguuugg auugugagcc ucgaaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaacaaua agcacuggcu gguccauaaa gaaugguucc acgacauccc acucccgugg     1260

cacgccggcg cagauacugg aacaccccau uggaacaaua aggaggcccu uguggaauuu     1320

aaagacgcuc acgcgaagcg ccagaccgua guugugcugg gcagccagga gggggcaguc     1380

cauaccgccu uggcuggagc ccuagaggca gaaauggaug gugccaaagg cagacugucc     1440

ucugggcacc ucaagugcag gcugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucauugugua cugcugccuu cacguuuaca aaaauuccug cagagacccu ccauggcacu     1560

gucaccgugg aaguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug     1620

gccguagaua ugcagacccu gacuccuguc ggccgcuuaa uaacagcaaa cccagugauc     1680

acggagagua ccgaaaacuc caaaaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaagaag auaacucacc acuggcauag aucuggauca     1800

acaauuggua aagccuuuga agcuaccguc aggggcgcca agcguauggc agugcucggg     1860

gacacagcuu gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc     1920

caccagaucu ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaca ccaaaaaugg uagcauuucc     2040

uugauguguc ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3918
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3918
gggagaaagc uuaccaugag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug cuauggcagc cgagguuacg cggcgcgguu ccgcguacua uauguaccug      120

gaucgaaacg acgcuggcga agccauuucu uuccccacca cauuggggau gaauaagugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgagugcccu      240

augcuggaug aaggcgugga gccagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaaa aagggggaag cccguagaag caggcgggcu      360

gugacacuac cgucccauuc uacccgcaaa cuccaaacga gaucgcagac uuggcuggag      420

ucaagggagu acaccaagca ccuuauucgg guugaaaauu ggaucuuucg caaccccgga      480

uucgcacugg ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug      600

agcaauagag auuuuguaga ggggaugucc ggagguacuu ggguugacau uguguuagaa      660

cauggcgggu gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcagaggug aggaguuacu gcuaugaagc uagcauaucu      780

gauauggccu ccgacucacg gugucccaca caaggagagg cguaccuuga caagcagagc      840

gauacccagu auguaugcaa acguacuuug guggaccgcg gcugggguaa ugggugugga      900

cuguucggca agggcagucu agucacaugc gccaaauuug caugcucuaa gaaaaugacc      960

gggaagucca uucagccuga aaaccucgag uacagaauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaaa ccgacgagaa cagggcuaag     1080

guggagauca caccaaauuc accccgggcc gaagcuacuc uugggggauu cggcucccug     1140

gguuuggauu gugagccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu ccauaaggaa ugguuccacg acaucccacu cccguggcac     1260

gccggcgcag auacuggaac accccauugg aacaauaaag aggcccuugu ggaauuuaag     1320

gacgcucacg cgaaacgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau     1380

accgccuugg cuggagcccu agaggcagaa auggauggug ccaagggcag acuguccucu     1440

gggcaccuca aaugcaggcu gaagauggac aagcugcggc ugaaaggagu gagcuauuca     1500

uuguguacug cugccuucac guuuacaaag auuccugcag agacccucca uggcacuguc     1560

accguggaag uucaguacgc ggguacagac gggccaugca aagugcccgc ucaaauggcc     1620

guagauaugc agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca     1800

auugguaagg ccuuugaagc uaccgucagg ggcgccaaac guauggcagu gcucggggac     1860

acagcuuggg auuucggaag uguaggcggu gcccugaaua gccuugggaa gggcauccac     1920

cagaucuucg gagcggccuu uaaauccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cucuuggggg aguguuaaua uuccugucaa cggcaguuag ugccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3919
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3919
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga gguuacgagg cgggguuccg cguacuauau guaccuggau      120

cgcaacgacg cuggcgaagc cauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga gugcccuaug      240

cuggaugaag gcguggagcc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaagccc gacguagcag aagggcugug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaguca      420

agagaguaca ccaagcaccu uauuaggguu gaaaauugga ucuuucggaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaggg gauguccgga gguacuuggg uugacauugu guuagaacau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaggugaga aguuacugcu augaagcuag cauaucugau      780

auggccuccg acucaaggug ucccacacaa ggagaggcgu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugaaaa ccucgaguac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugaaaccg acgagaacag agcuaaggug     1080

gagaucacac caaauucccc cagggccgaa gcuacucuug ggggauucgg cucacugggu     1140

uuggauugug agccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggaaugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaagagg cccuugugga auuuaaggac     1320

gcucacgcga aacgacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga ggcagaaaug gauggugcca agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaagauu ccugcagaga cccuccaugg cacugucacc     1560

guggaaguuc aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag caaacccagu gaucacggag     1680

aguaccgaaa acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggccu uugaagcuac cgucagaggc gccaaaagga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuc uugggggagu guuaauauuc cugucaacgg caguuagugc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3920
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3920
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaggu uacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

aacgacgcug gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug      240

gaugaaggcg uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caaaaagggg gaagcccgcc gaagccguag agcugugaca      360

cuaccguccc auucuaccag gaaacuccaa acgcggucgc agacuuggcu ggagucacgc      420

gaguacacca agcaccuuau uagaguugaa aauuggaucu uuaggaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc ggugcaucgg cgugagcaau      600

cgcgauuuug uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga ggugcgaagu uacugcuaug aagcuagcau aucugauaug      780

gccuccgacu caagaugucc cacacaagga gaggcguacc uugacaagca gagcgauacc      840

caguauguau gcaaaaggac uuugguggac cggggcuggg guaaugggug uggacuguuc      900

ggcaagggca gucuagucac augcgccaaa uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugaaaaccu cgaguaccgu aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gaaaccgacg agaaccgcgc uaagguggag     1080

aucacaccaa auucccccag agccgaagcu acucuugggg gauucggcuc acuggguuug     1140

gauugugagc cuaggaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aaacacuggc ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu     1320

cacgcgaaac ggcagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaggc agaaauggau ggugccaagg gccgacuguc cucugggcac     1440

cucaaaugcc gccugaagau ggacaagcug agacugaaag gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug     1560

gaaguucagu acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggcagguua auaacagcaa acccagugau cacggagagu     1680

accgaaaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcauc ggucuggauc aacaauuggu     1800

aaggccuuug aagcuaccgu ccgcggcgcc aaaagaaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc     1920

uucggagcgg ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcucuug ggggaguguu aauauuccug ucaacggcag uuagugccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3921
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3921
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccuuacaacu       60

gcuauggcag ccgagguuac gagaagggga uccgcguacu auauguaccu ggaucggaac      120

gacgcuggug aagccauuuc uuuccccacc acauugggca ugaauaagug cuauauacag      180

aucauggauu uagggcacau gugugacgca acuaugucau acgagugccc uaugcuggau      240

gaaggagugg agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uauggcacuu gucaucacaa aaagggugaa gcccgccgaa gccguagagc ugugacacua      360

ccgucccauu cuaccaggaa acuccaaacg cggucgcaga cuuggcugga gucacgcgag      420

uacaccaagc accuuauuag aguugaaaau uggaucuuua ggaaccccgg guucgcacug      480

gccgcugccg cgauugcaug guugcucgga agcaguacau cccagaaagu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucggu gcaucggcgu gagcaaucgc      600

gauuuuguag agggcauguc cgggggaacu uggguugaca uuguguuaga acaugguggc      660

ugugucaccg ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaggu gcgaaguuac ugcuaugaag cuagcauauc ugauauggcc      780

uccgacucaa gaugucccac acaaggggag gcguaccuug acaagcagag cgauacccag      840

uauguaugca aaaggacuuu gguggaccgg ggauggggca augguugugg gcuguucgga      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaaaaugac cggcaagucc      960

auucagccug aaaaccucga guaccguauc augcugagcg ugcacggguc gcaacacucu     1020

ggaaugauug uuaaugauac gggucaugaa accgacgaga accgcgcuaa gguggagauc     1080

acaccaaauu cccccagagc cgaagcuacu cuuggcgggu ucggaucacu gggcuuggau     1140

ugugagccua ggaccggucu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gacaucccac ucccguggca cgccggggca     1260

gauacuggca caccccauug gaacaauaaa gaggcccuug uggaauuuaa ggacgcucac     1320

gcgaaacggc agaccguagu ugugcuggga agccaggagg gcgcagucca uaccgccuug     1380

gcuggggccc uagaggcaga aauggaugga gccaaggguc gacuguccuc uggccaccuc     1440

aaaugccgcc ugaagaugga caaacuuaga cugaaggggg ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gagacccucc auggaacugu caccguggaa     1560

guucaguacg cgggcacaga cgguccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg gagguuaaua acagcaaacc cagugaucac ggagaguacc     1680

gaaaacucca agaugaugcu ggagcuagau cccccguucg gcgacagcua cauuguuauc     1740

ggagugggcg agaaaaagau aacucaccac uggcaucggu cugggucaac aauuggaaag     1800

gccuuugaag cuaccguccg cggugccaaa agaauggcag ugcucggcga cacagcuugg     1860

gauuucggga guguaggagg cgcccugaau agccuuggua aggggaucca ccagaucuuc     1920

ggcgcggccu uuaaauccuu guucggaggc augucuuggu uuucgcaaau ucugaucggg     1980

acucuccuga uguggcuugg acugaacacc aagaauggca gcauuuccuu gaugugucuc     2040

gcucugggug ggguguuaau auuccuguca acggcaguua gugccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3922
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3922
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu uacaacugcc       60

auggcugcag agguuacgag aaggggaucc gccuacuaua uguaccugga ucggaacgac      120

gcgggugaag cuauuucuuu ccccaccaca uugggcauga auaagugcua uauacagauc      180

auggauuuag ggcacaugug ugacgccacu augucauacg agugcccuau gcuggaugaa      240

ggaguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

ggcacuuguc aucacaaaaa gggugaagca cgccgaagcc guagagccgu gacacuaccg      360

ucccauucua ccaggaaacu ccaaacgcgg ucgcagacuu ggcuggaguc acgcgaguac      420

accaagcacc uuauuagagu ugaaaauugg aucuuuagga accccggguu cgcucuggca      480

gccgcugcca uugcgugguu gcucggaagc aguacauccc agaaagugau cuaucugguc      540

augauacugc ucaucgcacc cgccuacucu auucggugca ucggcgugag caaucgcgau      600

uuuguagagg gcauguccgg gggaacuugg guugacauug uguuagaaca ugguggcugu      660

gucaccguga uggcucagga uaagccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg ccgaggugcg aaguuacugc uaugaagcaa gcauaucuga uauggcuucc      780

gacucaagau gucccacaca aggggaggcc uaccuugaca agcagagcga uacccaguau      840

guaugcaaaa ggacuuuggu ggaccgggga uggggcaaug guugugggcu guucggaaag      900

ggcagucuag ucacaugcgc gaaauuugcc ugcucuaaga aaaugaccgg caaguccauu      960

cagccugaaa accucgagua ccguaucaug cugagcgugc acgggucgca acacucugga     1020

augauuguua augauacggg ucaugaaacc gacgagaacc gcgcaaaggu ggagaucaca     1080

ccaaauuccc ccagagcuga agccacucuu ggcggguucg gaucacuggg cuuggauugu     1140

gagccuagga ccggucucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc uggggccgau     1260

acuggcaccc cucauuggaa caauaaagag gcacuugugg aauuuaagga cgcccacgcu     1320

aaacggcaga caguaguugu gcugggaagc caggagggcg cgguccauac cgcauuggcc     1380

ggggcucuag aggccgaaau ggauggagca aagggucgac uguccucugg ccaccucaaa     1440

ugccgccuga agauggacaa acuuagacug aaggggguga gcuauucauu guguacugcc     1500

gcuuucacgu uuacaaagau uccugccgag acccuccaug gaacugucac cguggaaguu     1560

caguacgcag gcacagacgg uccaugcaaa gugcccgcgc aaauggcugu agauaugcag     1620

acccugacuc cugucgggag guuaauaaca gccaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggcg acagcuacau uguuaucgga     1740

gugggcgaga aaaagauaac ucaccacugg caucggucug ggucaacaau uggaaaggca     1800

uuugaagcca ccguccgcgg ugcuaaaaga auggccgugc ucggcgacac agcaugggau     1860

uucgggagug uaggaggcgc ucugaauagc cuugguaagg ggauccacca gaucuucggc     1920

gccgcguuua aauccuuguu cggaggcaug ucuugguuuu cgcaaauucu gaucgggacu     1980

cuccugaugu ggcuuggacu gaacaccaag aauggcagca uuuccuugau gugucucgcc     2040

cugggugggg uguuaauauu ccugucaacg gcuguuagug cagacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  3923
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3923
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcag auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3924
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3924
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagccg uuacgagaag gggauccgcg uacuauaugu accuggaccg gaacgaugcu      120

ggugaggcca uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcaacuaug ucauacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaggcccgc cgaagccgua gagcugugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggaaucacg cgaguacacc      420

aagcaccuua uuagaguuga gaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagaaggca uguccggggg aacuuggguu gauauugugu uagagcaugg uggcuguguc      660

accgugaugg cccaggacaa gccaacagug gauaucgaac uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaggcuagca uaucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaagcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugagaacc ucgaauaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugagaccgau gaaaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaggc uacucuuggc ggguucggau cacugggcuu ggacugugaa     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggagugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacacu     1260

ggcacacccc auuggaacaa uaaagaagcc cuuguggagu uuaaggaugc ucacgcgaaa     1320

cggcagaccg uaguugugcu gggaagccag gaaggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagagaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagaaacc cuccauggaa cugucaccgu ggagguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaggcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccg auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3925
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3925
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccgc gguuacgagg cgggguuccg cuuacuauau guaccuggau      120

cgcaacgacg ccggcgaggc aauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgccacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaggcuc gacguagcag aagggcagug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaauca      420

agagaguaca ccaagcaccu uauuaggguu gagaauugga ucuuucggaa ccccggauuc      480

gcccuggcug ccgcggcaau ugccugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgcuccu gccuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcacaggau aagccaacag uggacaucga acuggucacc      720

acgacuguuu caaacauggc ugaggugaga aguuacugcu augaggccag cauaucugau      780

auggcguccg acucaaggug ucccacacaa ggagaagccu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgca aaauuugcuu gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgaaaacag agccaaggug     1080

gagaucacac caaauucccc cagggcugag gccacucuug ggggauucgg cucacugggu     1140

uuggauugug aaccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgca     1260

ggcgccgaua cuggaacacc ccauuggaac aauaaagaag cucuugugga guuuaaggac     1320

gcgcacgcaa aacgacagac cguaguugug cugggcagcc aggaaggggc cguccauacc     1380

gcuuuggccg gagcacuaga ggccgagaug gauggugcua agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugccg cauucacguu uacaaagauu ccugcggaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcugg uacagacggg ccaugcaaag ugcccgccca aauggcagua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag ccaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggaa cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggcuu uugaggccac cgucagaggc gcaaaaagga uggcugugcu cggggacaca     1860

gccugggauu ucggaagugu aggcggugcg cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag ccgcuuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcac uugggggagu guuaauauuc cugucaacgg ccguuagugc ugacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3926
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3926
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagguguuac ucgacguggc      120

ucagccuacu auauguaccu ggauagaaac gacgcugggg aggcaauuag uuuucccacg      180

acccuaggaa ugaauaaaug cuauauacaa aucauggauc ucggccauau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggugugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugggaccu gucaccauaa gaaaggagag      360

gcgaggcggu cucgcagagc ugugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg cuuugcccug gcagccgcug caauugccug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc uccugccuac      600

uccauuaggu gcaucggggu gucaaaucgg gauuucguag aaggaaugag ugguggcaca      660

uggguugaca uuguguugga gcacggggga ugugucacug ugauggcgca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggcagaggu gcguucuuac      780

ugcuaugagg ccuccauauc agauauggcu agcgacaguc gcugucccac ucagggcgaa      840

gccuaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

gguuggggga auggaugugg ccuguuuggc aaaggguccc uugucacuug cgcaaaguuc      960

gcuugcagca aaaagaugac aggaaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacgguuc ccagcauuca ggcaugauug uuaaugauac cgggcacgag     1080

acggacgaaa accgagccaa aguggagauc accccaaaua guccccgcgc ggaggccaca     1140

cucggaggcu uugguagccu gggguuagau ugugaaccua gaacuggccu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcaggagcu gauacaggca cuccccacug gaacaauaag     1320

gaagcccuag uggaguucaa agacgcucau gccaagaggc agaccguagu ugugcugggg     1380

ucucaggaag gagcagucca cacagcccuc gcuggugcgc uggaggcaga gauggauggc     1440

gccaaagggc ggcuuagcuc aggacaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggcg ugaguuauuc cuuauguacc gcugccuuua cuuucacgaa aauuccugca     1560

gaaacacugc acgguaccgu cacuguggag guucaauacg ccgggaccga cggcccaugc     1620

aaggugcccg cucagauggc cguagauaug cagacacuaa ccccugucgg aaggcucaua     1680

acugcaaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gcgacucaua cauuguuauc ggggugggag agaagaagau aacccaccau     1800

uggcaccgua gugguagcac uauuggcaaa gcguucgagg cuacaguccg aggggccaag     1860

cggauggcag uguugggaga caccgccugg gauuucggcu ccguaggugg ggcucucaau     1920

ucucugggca aaggaaucca ucaaaucuuu ggcgccgcau ucaagucgcu guuuggggga     1980

augagcuggu ucucccagau ucuuaucggc acacuguuga uguggcucgg ucugaacacu     2040

aaaaaugggu caauuaguuu aaugugucug gcucuaggag gcgugcucau auuucugucu     2100

accgccguua gcgcggacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3927
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3927
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagccguuac ucgacguggu      120

ucagcuuacu auauguaccu ggauagaaac gacgcaggcg aggccauuag uuuucccacg      180

acccuaggga ugaauaaaug cuauauacaa aucauggauc ucggacauau gugugacgcg      240

acaaugagcu acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugguaccu gucaccauaa gaaaggggag      360

gcuaggcggu cucgcagagc cgugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg auuugcacug gccgcugcag ccauugcuug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc cccugcguac      600

uccauuaggu gcaucggcgu gucaaaucgg gauuucguag aagggaugag uggagguaca      660

uggguugaca uuguguugga gcacggcggg ugugucacug ugauggcaca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggccgaggu gcguucuuac      780

ugcuaugagg cuuccauauc agauauggcc agcgacaguc gcugucccac ucagggagaa      840

gcauaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

ggcuggggua augggugugg acuguuuggc aaaggcuccc uugucacuug cgcuaaguuc      960

gccugcagca aaaagaugac agggaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacggauc ccagcauuca gguaugauug uuaaugauac cggccacgag     1080

acggacgaaa accgagcgaa aguggagauc accccaaaua guccccgcgc cgaggcaaca     1140

cucgggggau uuggcagccu ggguuuagau ugugaaccua gaacugggcu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcuggcgcc gauacaggaa cuccccacug gaacaauaag     1320

gaagcucuag uggaguucaa agacgcccau gcaaagaggc agaccguagu ugugcugggc     1380

ucucaggaag gggccgucca cacagcucuc gcgggagcac uggaggccga gauggauggu     1440

gcuaaaggcc ggcuuagcuc agggcaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggag ugaguuauuc cuuauguacc gccgcauuua cuuucacgaa aauuccugcc     1560

gaaacacugc acggcaccgu cacuguggag guucaauacg cugguaccga cgggccaugc     1620

aaggugcccg cccagauggc aguagauaug cagacacuaa ccccugucgg caggcucaua     1680

acugcgaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gagacucaua cauuguuauc ggcguggggg agaagaagau aacccaccau     1800

uggcaccgua guggaagcac uauugguaag gcuuucgagg ccacaguccg gggcgcaaaa     1860

cgaauggccg uguuggggga caccgcuugg gauuucggau ccguaggcgg ugcccucaau     1920

ucucugggga aaggcaucca ucaaaucuuu ggagcagcuu ucaagucgcu guuuggcggg     1980

augagcuggu ucucccagau ucuuaucgga acacuguuga uguggcucgg ccugaacacu     2040

aaaaaugguu caauuaguuu aaugugucug gcccuagggg gagugcucau auuucugucu     2100

accgcgguua gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3928
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3928
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcuugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  3929
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3929
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agaggcgggg cgcugacacc       60

agcgugggga uugucggacu gcuccuuaca acugcaaugg ccgcggaagu uacgcgccga      120

gguuccgcuu acuauaugua ccuggaucgu aaugacgccg gcgaggcaau aucuuucccc      180

accacauugg ggaugaacaa augcuauauc cagauuaugg auuuaggaca caugugugac      240

gccacuaugu cauacgaaug cccuaugcug gaugagggcg uggaaccaga cgauguagac      300

uguuggugca auaccacaag uaccugggug gucuauggua cuugucauca caagaaaggg      360

gaggcuagaa ggagccggcg cgcagugaca cuaccguccc auucuaccag aaagcuccaa      420

acgaggucgc agacuuggcu ggagucacgg gaauacacca agcaccuuau ccgcguugag      480

aacuggauuu uucgaaaucc cggauucgcc cuggcugccg cggcaaucgc cugguugcuc      540

ggcagcagua caucccagaa agugauauau cuggucauga uccugcucau ugcuccugcc      600

uacucuauca gaugcauugg cgugagcaac agggauuuug uagaagggau guccggaggu      660

acuuggguug acaucguguu agagcauggc ggguguguca ccgugauggc acaggauaag      720

ccaacagugg acauagagcu ggucaccacg acuguuucaa auauggcuga agugcggagu      780

uacugcuaug aggccagcau uucugauaug gcguccgacu cacguugucc cacacaagga      840

gaagccuacc uugacaaaca gagcgauacc caguauguau gcaagcgcac uuugguggac      900

agaggcuggg guaacgggug uggacuguuc ggcaaaggca gucuagucac augcgcaaag      960

uuugcuugcu cuaagaaaau gaccgggaag uccauccagc cugagaaucu cgaauacagg     1020

auuaugcuga gcgugcacgg aucgcaacac ucugguauga ucguuaacga uacgggccau     1080

gagaccgacg agaaucgggc caaaguggaa aucacaccaa acucaccccg agcugaggcc     1140

acucuugggg gauucggcuc ccuggguuug gauugugaac cucgcaccgg gcucgacuuu     1200

agugaucugu auuacuuaac aaugaauaac aagcacuggc ugguccauaa agagugguuc     1260

cacgacauuc cacucccgug gcacgcaggc gccgauacug gaacacccca uuggaauaac     1320

aaggaagcuc uuguggaguu uaaagacgcg cacgcaaaga gacagaccgu aguugugcug     1380

ggcagccagg agggggccgu ccauaccgcu uuggccggag cacuagaagc cgagauggau     1440

ggugcuaagg gcaggcuguc cucugggcac cucaaaugcc ggcugaagau ggacaaacuu     1500

cgccugaagg gagugagcua uucauugugu acugccgcau ucacguuuac aaaaauaccu     1560

gcggaaaccc uccauggcac ugucaccgug gagguucagu acgcugguac agacgggcca     1620

ugcaaggugc ccgcccaaau ggcaguagau augcagaccc ugacuccugu cggcagauua     1680

aucacagcca acccagugau uacggaaagu accgaaaauu ccaagaugau gcuggagcua     1740

gaucccccgu ucggagacag cuacaucguu auaggcgugg gggagaaaaa gauuacucac     1800

cacuggcaua ggucuggauc aacaaucggu aaggcuuuug aggccaccgu ccguggcgca     1860

aaacggaugg cugugcucgg ggacacagcc ugggauuucg gaaguguagg cggugcgcug     1920

aacagccuug ggaaaggcau ccaccagauu uucggagccg cuuuuaaguc cuuguucggc     1980

gggaugucuu gguuuucgca aauccugauu ggaacucucc ugauguggcu uggccugaau     2040

accaagaacg guagcauauc cuugaugugu cucgcauuag ggggagugcu gaucuuccug     2100

ucaacggccg uuagugcuug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  3930
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3930
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagaggcg gggcgcugac       60

accagcgugg ggauagucgg acugcuccuu acaacugcaa uggccgcgga aguuacgcgc      120

cgagguuccg cuuacuauau guaccuggau cguaaugacg ccggcgaggc aaucucuuuc      180

cccaccacau uggggaugaa caaaugcuau auucagauca uggauuuagg acacaugugu      240

gacgccacua ugucauacga augcccuaug cuggaugagg gcguggaacc agacgaugua      300

gacuguuggu gcaauaccac aaguaccugg guggucuaug guacuuguca ucacaagaaa      360

ggggaggcua gaaggagccg gcgcgcagug acacuaccgu cccauucuac cagaaagcuc      420

caaacgaggu cgcagacuug gcuggaguca cgggaauaca ccaagcaccu uauucgcguu      480

gagaacugga ucuuucgaaa ucccggauuc gcccuggcug ccgcggcaau agccugguug      540

cucggcagca guacauccca gaaagugauc uaucugguca ugauucugcu caucgcuccu      600

gccuacucua uuagaugcau cggcgugagc aacagggauu uuguagaagg gauguccgga      660

gguacuuggg uugacauagu guuagagcau ggcgggugug ucaccgugau ggcacaggau      720

aagccaacag uggacauuga gcuggucacc acgacuguuu caaauauggc ugaagugcgg      780

aguuacugcu augaggccag caucucugau auggcguccg acucacguug ucccacacaa      840

ggagaagccu accuugacaa acagagcgau acccaguaug uaugcaagcg cacuuuggug      900

gacagaggcu gggguaacgg guguggacug uucggcaaag gcagucuagu cacaugcgca      960

aaguuugcuu gcucuaagaa aaugaccggg aaguccauuc agccugagaa ucucgaauac     1020

aggaucaugc ugagcgugca cggaucgcaa cacucuggua ugaucguuaa cgauacgggc     1080

caugagaccg acgagaaucg ggccaaagug gaaauuacac caaacucacc ccgagcugag     1140

gccacucuug ggggauucgg cucccugggu uuggauugug aaccucgcac cgggcucgac     1200

uuuagugauc uguauuacuu aacaaugaau aacaagcacu ggcuggucca uaaagagugg     1260

uuccacgaca uaccacuccc guggcacgca ggcgccgaua cuggaacacc ccauuggaau     1320

aacaaggaag cucuugugga guuuaaagac gcgcacgcaa agagacagac cguaguugug     1380

cugggcagcc aggagggggc cguccauacc gcuuuggccg gagcacuaga agccgagaug     1440

gauggugcua agggcaggcu guccucuggg caccucaaau gccggcugaa gauggacaaa     1500

cuucgccuga agggagugag cuauucauug uguacugccg cauucacguu uacaaaaauc     1560

ccugcggaaa cccuccaugg cacugucacc guggagguuc aguacgcugg uacagacggg     1620

ccaugcaagg ugcccgccca aauggcagua gauaugcaga cccugacucc ugucggcaga     1680

uuaauuacag ccaacccagu gaucacggaa aguaccgaaa auuccaagau gaugcuggag     1740

cuagaucccc cguucggaga cagcuacaua guuauuggcg ugggggagaa aaagaucacu     1800

caccacuggc auaggucugg aucaacaauc gguaaggcuu uugaggccac cguccguggc     1860

gcaaaacgga uggcugugcu cggggacaca gccugggauu ucggaagugu aggcggugcg     1920

cugaacagcc uugggaaagg cauucaccag aucuucggag ccgcuuuuaa guccuuguuc     1980

ggcgggaugu cuugguuuuc gcaaauucug auaggaacuc uccugaugug gcuuggccug     2040

aauaccaaga acgguagcau cuccuugaug ugucucgcau uagggggagu gcugauuuuc     2100

cugucaacgg ccguuagugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  3931
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3931
gggagaaagc uuaccaugaa cgccagaaag gagaaaaaga ggcggggcgc ugacaccagc       60

guggggaucg ucggacugcu ccuuacaacu gcaauggccg cggaaguuac gcgccgaggu      120

uccgcuuacu auauguaccu ggaucguaau gacgccggcg aggcaauuuc uuuccccacc      180

acauugggga ugaacaaaug cuauauacag aucauggauu uaggacacau gugugacgcc      240

acuaugucau acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaua ccacaaguac cugggugguc uaugguacuu gucaucacaa gaaaggggag      360

gcuagaagga gccggcgcgc agugacacua ccgucccauu cuaccagaaa gcuccaaacg      420

aggucgcaga cuuggcugga gucacgggaa uacaccaagc accuuauucg cguugagaac      480

uggaucuuuc gaaaucccgg auucgcccug gcugccgcgg caauugccug guugcucggc      540

agcaguacau cccagaaagu gaucuaucug gucaugauac ugcucaucgc uccugccuac      600

ucuauuagau gcaucggcgu gagcaacagg gauuuuguag aagggauguc cggagguacu      660

uggguugaca uuguguuaga gcauggcggg ugugucaccg ugauggcaca ggauaagcca      720

acaguggaca ucgagcuggu caccacgacu guuucaaaua uggcugaagu gcggaguuac      780

ugcuaugagg ccagcauauc ugauauggcg uccgacucac guugucccac acaaggagaa      840

gccuaccuug acaaacagag cgauacccag uauguaugca agcgcacuuu gguggacaga      900

ggcuggggua acgggugugg acuguucggc aaaggcaguc uagucacaug cgcaaaguuu      960

gcuugcucua agaaaaugac cgggaagucc auucagccug agaaucucga auacaggauc     1020

augcugagcg ugcacggauc gcaacacucu gguaugauug uuaacgauac gggccaugag     1080

accgacgaga aucgggccaa aguggaaauc acaccaaacu caccccgagc ugaggccacu     1140

cuugggggau ucggcucccu ggguuuggau ugugaaccuc gcaccgggcu cgacuuuagu     1200

gaucuguauu acuuaacaau gaauaacaag cacuggcugg uccauaaaga gugguuccac     1260

gacaucccac ucccguggca cgcaggcgcc gauacuggaa caccccauug gaauaacaag     1320

gaagcucuug uggaguuuaa agacgcgcac gcaaagagac agaccguagu ugugcugggc     1380

agccaggagg gggccgucca uaccgcuuug gccggagcac uagaagccga gauggauggu     1440

gcuaagggca ggcuguccuc ugggcaccuc aaaugccggc ugaagaugga caaacuucgc     1500

cugaagggag ugagcuauuc auuguguacu gccgcauuca cguuuacaaa aauuccugcg     1560

gaaacccucc auggcacugu caccguggag guucaguacg cugguacaga cgggccaugc     1620

aaggugcccg cccaaauggc aguagauaug cagacccuga cuccugucgg cagauuaaua     1680

acagccaacc cagugaucac ggaaaguacc gaaaauucca agaugaugcu ggagcuagau     1740

cccccguucg gagacagcua cauuguuauc ggcguggggg agaaaaagau aacucaccac     1800

uggcauaggu cuggaucaac aauugguaag gcuuuugagg ccaccguccg uggcgcaaaa     1860

cggauggcug ugcucgggga cacagccugg gauuucggaa guguaggcgg ugcgcugaac     1920

agccuuggga aaggcaucca ccagaucuuc ggagccgcuu uuaaguccuu guucggcggg     1980

augucuuggu uuucgcaaau ucugaucgga acucuccuga uguggcuugg ccugaauacc     2040

aagaacggua gcauuuccuu gaugugucuc gcauuagggg gagugcugau auuccuguca     2100

acggccguua gugcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3932
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3932
gggagaaagc uuaccauggc cagaaaggag aaaaagaggc ggggcgcuga caccagcgug       60

gggaucgucg gacugcuccu uacaacugca auggccgcgg aaguuacgcg ccgagguucc      120

gcuuacuaua uguaccugga ucguaacgac gccggcgagg caauuucuuu ccccaccaca      180

uuggggauga auaaaugcua uauacagauc auggauuuag gacacaugug ugacgccacu      240

augucauacg aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg      300

ugcaacacca caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu      360

agaaggagcc ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccaaacgagg      420

ucgcagacuu ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaauugg      480

aucuuucgaa accccggauu cgcccuggcu gccgcggcaa uugccugguu gcucggcagc      540

aguacauccc agaaagugau cuaucugguc augauacugc ucaucgcucc ugccuacucu      600

auuagaugca ucggcgugag caauagggau uuuguagaag ggauguccgg agguacuugg      660

guugacauug uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca      720

guggacaucg agcuggucac cacgacuguu ucaaacaugg cugaagugcg gaguuacugc      780

uaugaggcca gcauaucuga uauggcgucc gacucacguu gucccacaca aggagaagcc      840

uaccuugaca aacagagcga uacccaguau guaugcaagc gcacuuuggu ggacagaggc      900

ugggguaaug gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu      960

ugcucuaaga aaaugaccgg gaaguccauu cagccugaga accucgaaua caggaucaug     1020

cugagcgugc acggaucgca acacucuggu augauuguua augauacggg ccaugagacc     1080

gacgagaacc gggccaaagu ggaaaucaca ccaaauucac cccgagcuga ggccacucuu     1140

gggggauucg gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau     1200

cuguauuacu uaacaaugaa caauaagcac uggcuggucc auaaagagug guuccacgac     1260

aucccacucc cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa     1320

gcucuugugg aguuuaaaga cgcgcacgca aagagacaga ccguaguugu gcugggcagc     1380

caggaggggg ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu     1440

aagggcaggc uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcauucacgu uuacaaaaau uccugcggaa     1560

acccuccaug gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag     1620

gugcccgccc aaauggcagu agauaugcag acccugacuc cugucggcag auuaauaaca     1680

gccaacccag ugaucacgga aaguaccgag aacuccaaga ugaugcugga gcuagauccc     1740

ccguucggag acagcuacau uguuaucggc gugggggaga aaaagauaac ucaccacugg     1800

cauaggucug gaucaacaau ugguaaggcu uuugaagcca ccguccgugg cgcaaaacgg     1860

auggcugugc ucggggacac agccugggau uucggaagug uaggcggugc gcugaauagc     1920

cuugggaaag gcauccacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug     1980

ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag     2040

aaugguagca uuuccuugau gugucucgca uuagggggag ugcugauauu ccugucaacg     2100

gccguuagug cuugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  3933
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3933
gggagaaagc uuaccaugag aaaggagaaa aagaggcggg gcgccgacac cagcgugggg       60

aucgucggac ugcuccuuac aacugcuaug gcagccgaag uuacgcgccg agguuccgcg      120

uacuauaugu accuggaucg uaacgacgcu ggcgaggcca uuucuuuccc caccacauug      180

gggaugaaua aaugcuauau acagaucaug gauuuaggac acauguguga cgcaacuaug      240

ucauacgaau gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc      300

aacaccacaa guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggccaga      360

aggagccggc gcgcugugac acuaccgucc cauucuacca gaaagcucca aacgaggucg      420

cagacuuggc uggagucacg ggaauacacc aagcaccuua uucgcguuga gaauuggauc      480

uuucgaaacc ccggauucgc acuggccgcu gccgcgauug caugguugcu cggcagcagu      540

acaucccaga aagugaucua ucuggucaug auacugcuca ucgccccugc uuacucuauu      600

agaugcaucg gcgugagcaa uagggauuuu guagaaggga uguccggagg uacuuggguu      660

gacauugugu uagagcaugg cggguguguc accgugaugg cccaggauaa gccaacagug      720

gacaucgagc uggucaccac gacuguuuca aacauggcag aagugcggag uuacugcuau      780

gaggcuagca uaucugauau ggccuccgac ucacguuguc ccacacaagg agaagcguac      840

cuugacaaac agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg      900

gguaaugggu guggacuguu cggcaaaggc agucuaguca caugcgccaa guuugcaugc      960

ucuaagaaaa ugaccgggaa guccauucag ccugagaacc ucgaauacag gaucaugcug     1020

agcgugcacg gaucgcaaca cucugguaug auuguuaaug auacgggcca ugagaccgac     1080

gagaaccggg cuaaagugga aaucacacca aauucacccc gagccgaggc uacucuuggg     1140

ggauucggcu cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug     1200

uauuacuuaa caaugaacaa uaagcacugg cugguccaua aagagugguu ccacgacauc     1260

ccacucccgu ggcacgccgg cgcagauacu ggaacacccc auuggaacaa uaaggaagcc     1320

cuuguggagu uuaaagacgc ucacgcgaag agacagaccg uaguugugcu gggcagccag     1380

gagggggcag uccauaccgc cuuggcugga gcccuagaag cagagaugga uggugccaag     1440

ggcaggcugu ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag     1500

ggagugagcu auucauugug uacugcugcc uucacguuua caaaaauucc ugcagaaacc     1560

cuccauggca cugucaccgu ggagguucag uacgcgggua cagacgggcc augcaaggug     1620

cccgcucaaa uggccguaga uaugcagacc cugacuccug ucggcagauu aauaacagca     1680

aacccaguga ucacggaaag uaccgagaac uccaagauga ugcuggagcu agaucccccg     1740

uucggagaca gcuacauugu uaucggcgug ggggagaaaa agauaacuca ccacuggcau     1800

aggucuggau caacaauugg uaaagccuuu gaagcuaccg uccguggcgc caagcggaug     1860

gcagugcucg gggacacagc uugggauuuc ggaaguguag gcggugcccu gaauagccuu     1920

gggaaaggca uccaccagau cuucggagcg gccuuuaagu ccuuguucgg cgggaugucu     1980

ugguuuucgc aaauucugau cggaacucuc cugauguggc uuggccugaa caccaagaau     2040

gguagcauuu ccuugaugug ucucgcuuua gggggagugc ugauauuccu gucaacggca     2100

guuagugccu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  3934
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3934
gggagaaagc uuaccaugaa ggagaaaaag agaaggggcg ccgacaccag cguggggauc       60

gucggacugc uccuuacaac ugcuauggca gccgaaguua cgcggcgcgg uuccgcguac      120

uauauguacc uggaucgaaa cgacgcuggc gaggccauuu cuuuccccac cacauugggg      180

augaauaaau gcuauauaca gaucauggau uuaggacaca ugugugacgc aacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcccguaga      360

agcaggcggg cugugacacu accgucccau ucuacccgca agcuccaaac gagaucgcag      420

acuuggcugg agucaaggga auacaccaag caccuuauuc ggguugagaa uuggaucuuu      480

cgcaaccccg gauucgcacu ggccgcugcc gcgauugcau gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugaua cugcucaucg ccccugcuua cucuauucga      600

ugcaucggcg ugagcaauag agauuuugua gaagggaugu ccggagguac uuggguugac      660

auuguguuag agcauggcgg gugugucacc gugauggccc aggauaagcc aacaguggac      720

aucgagcugg ucaccacgac uguuucaaac auggcagaag ugaggaguua cugcuaugag      780

gcuagcauau cugauauggc cuccgacuca cgguguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guauguaugc aagcguacuu ugguggaccg cggcuggggu      900

aaugggugug gacuguucgg caaaggcagu cuagucacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaccucg aauacagaau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauu guuaaugaua cgggccauga gaccgacgag     1080

aacagggcua aaguggaaau cacaccaaau ucaccccggg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagcgc cagaccguag uugugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

agacuguccu cugggcaccu caaaugcagg cugaagaugg acaaacuucg gcugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauuccugc agaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gccgcuuaau aacagcaaac     1680

ccagugauca cggaaaguac cgagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauuguuau cggcgugggg gagaaaaaga uaacucacca cuggcauaga     1800

ucuggaucaa caauugguaa agccuuugaa gcuaccguca ggggcgccaa gcguauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

agcauuuccu ugaugugucu cgcuuuaggg ggagugcuga uauuccuguc aacggcaguu     2100

agugccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  3935
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3935
gggagaaagc uuaccaugga gaagaaaaga aggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc uuacaacugc uauggcagcc gaaguuacgc ggcgcgguuc cgcguacuau      120

auguaccugg aucgaaacga cgcuggcgag gccauuucuu uccccaccac auuggggaug      180

aauaagugcu auauacagau cauggauuua ggacacaugu gugacgcaac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaaa agggggaggc ccguagaagc      360

aggcgggcug ugacacuacc gucccauucu acccgcaaac uccaaacgag aucgcagacu      420

uggcuggagu caagggaaua caccaagcac cuuauucggg uugagaauug gaucuuucgc      480

aaccccggau ucgcacuggc cgcugccgcg auugcauggu ugcucggcag caguacaucc      540

cagaagguga ucuaucuggu caugauacug cucaucgccc cugcuuacuc uauucgaugc      600

aucggcguga gcaauagaga uuuuguagaa gggauguccg gagguacuug gguugacauu      660

guguuagagc auggcgggug ugucaccgug auggcccagg auaaaccaac aguggacauc      720

gagcugguca ccacgacugu uucaaacaug gcagaaguga ggaguuacug cuaugaggcu      780

agcauaucug auauggccuc cgacucacgg ugucccacac aaggagaagc guaccuugac      840

aagcagagcg auacccagua uguaugcaaa cguacuuugg uggaccgcgg cugggguaau      900

ggguguggac uguucggcaa gggcagucua gucacaugcg ccaaauuugc augcucuaag      960

aagaugaccg ggaaauccau ucagccugag aaccucgaau acagaaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugauuguu aaugauacgg gccaugagac cgacgagaac     1080

agggcuaagg uggaaaucac accaaauuca ccccgggccg aggcuacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucga accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga acaauaaaca cuggcugguc cauaaggagu gguuccacga caucccacuc     1260

ccguggcacg ccggcgcaga uacuggaaca ccccauugga acaauaaaga agcccuugug     1320

gaguuuaagg acgcucacgc gaaacgccag accguaguug ugcugggcag ccaggagggg     1380

gcaguccaua ccgccuuggc uggagcccua gaagcagaga uggauggugc caagggcaga     1440

cuguccucug ggcaccucaa gugcaggcug aaaauggaca agcugcggcu gaaaggagug     1500

agcuauucau uguguacugc ugccuucacg uuuacaaaga uuccugcaga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcg gguacagacg ggccaugcaa agugcccgcu     1620

caaauggccg uagauaugca gacccugacu ccugucggcc gcuuaauaac agcaaaccca     1680

gugaucacgg aaaguaccga gaacuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uuguuaucgg cgugggggaa aagaaaauaa cucaccacug gcauagaucu     1800

ggaucaacaa uugguaaggc cuuugaggcu accgucaggg gcgccaaacg uauggcagug     1860

cucggggaca cagcuuggga uuucggaagu guaggcggug cccugaauag ccuugggaag     1920

ggcauccacc agaucuucgg agcggccuuu aaauccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugaucggaac ucuccugaug uggcuuggcc ugaacaccaa gaaugguagc     2040

auuuccuuga ugugucucgc ucuuggggga guguuaauau uccugucaac ggcaguuagu     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  3936
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3936
gggagaaagc uuaccaugaa gaaaagaagg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuua caacugcuau ggcagccgag guuacgcggc gcgguuccgc guacuauaug      120

uaccuggauc gaaacgacgc uggcgaagcc auuucuuucc ccaccacauu ggggaugaau      180

aagugcuaua uacagaucau ggauuuagga cacaugugug acgcaacuau gucauacgag      240

ugcccuaugc uggaugaagg cguggagcca gacgauguag acuguuggug caacaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaaaaagg gggaagcccg uagaagcagg      360

cgggcuguga cacuaccguc ccauucuacc cgcaaacucc aaacgagauc gcagacuugg      420

cuggagucaa gggaguacac caagcaccuu auucggguug aaaauuggau cuuucgcaac      480

cccggauucg cacuggccgc ugccgcgauu gcaugguugc ucggcagcag uacaucccag      540

aaggugaucu aucuggucau gauacugcuc aucgccccug cuuacucuau ucgaugcauc      600

ggcgugagca auagagauuu uguagagggg auguccggag guacuugggu ugacauugug      660

uuagaacaug gcgggugugu caccgugaug gcccaggaua aaccaacagu ggacaucgag      720

cuggucacca cgacuguuuc aaacauggca gaggugagga guuacugcua ugaagcuagc      780

auaucugaua uggccuccga cucacggugu cccacacaag gagaggcgua ccuugacaag      840

cagagcgaua cccaguaugu augcaaacgu acuuuggugg accgcggcug ggguaauggg      900

uguggacugu ucggcaaggg cagucuaguc acaugcgcca aauuugcaug cucuaagaag      960

augaccggga aauccauuca gccugaaaac cucgaguaca gaaucaugcu gagcgugcac     1020

ggaucgcaac acucugguau gauuguuaau gauacgggcc augaaaccga cgagaacagg     1080

gcuaaggugg agaucacacc aaauucaccc cgggccgaag cuacucuugg gggauucggc     1140

ucccuggguu uggauuguga gccucgaacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgacau cccacucccg     1260

uggcacgccg gcgcagauac uggaacaccc cauuggaaca auaaagaggc ccuuguggaa     1320

uuuaaggacg cucacgcgaa acgccagacc guaguugugc ugggcagcca ggagggggca     1380

guccauaccg ccuuggcugg agcccuagag gcagaaaugg auggugccaa gggcagacug     1440

uccucugggc accucaagug caggcugaaa auggacaagc ugcggcugaa aggagugagc     1500

uauucauugu guacugcugc cuucacguuu acaaagauuc cugcagagac ccuccauggc     1560

acugucaccg uggaaguuca guacgcgggu acagacgggc caugcaaagu gcccgcucaa     1620

auggccguag auaugcagac ccugacuccu gucggccgcu uaauaacagc aaacccagug     1680

aucacggaga guaccgaaaa cuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacauug uuaucggcgu gggggagaag aaaauaacuc accacuggca uagaucugga     1800

ucaacaauug guaaggccuu ugaagcuacc gucaggggcg ccaaacguau ggcagugcuc     1860

ggggacacag cuugggauuu cggaagugua ggcggugccc ugaauagccu ugggaagggc     1920

auccaccaga ucuucggagc ggccuuuaaa uccuuguucg gcgggauguc uugguuuucg     1980

caaauucuga ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu     2040

uccuugaugu gucucgcucu ugggggagug uuaauauucc ugucaacggc aguuagugcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  3937
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3937
gggagaaagc uuaccaugaa gagaaggggc gccgacacca gcguggggau cgucggacug       60

cuccuuacaa cugcuauggc agccgagguu acgcggcgcg guuccgcgua cuauauguac      120

cuggaucgaa acgacgcugg cgaagccauu ucuuucccca ccacauuggg gaugaauaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg caacuauguc auacgagugc      240

ccuaugcugg augaaggcgu ggagccagac gauguagacu guuggugcaa caccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aagcccguag aagcaggcgg      360

gcugugacac uaccguccca uucuacccgc aagcuccaaa cgagaucgca gacuuggcug      420

gagucaaggg aguacaccaa acaccuuauu cggguugaaa auuggaucuu ucgcaacccc      480

ggauucgcac uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaag      540

gugaucuauc uggucaugau acugcucauc gccccugcuu acucuauucg augcaucggc      600

gugagcaaua gagauuuugu agaggggaug uccggaggua cuuggguuga cauuguguua      660

gaacauggcg ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa cauggcagag gugaggaguu acugcuauga agcuagcaua      780

ucugauaugg ccuccgacuc acgguguccc acacaaggag aggcguaccu ugacaaacag      840

agcgauaccc aguauguaug caagcguacu uugguggacc gcggcugggg uaaugggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaaaaagaug      960

accgggaagu ccauucagcc ugaaaaccuc gaguacagaa ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaaugau acgggccaug aaaccgacga gaacagggcu     1080

aaaguggaga ucacaccaaa uucaccccgg gccgaagcua cucuuggggg auucggcucc     1140

cuggguuugg auugugagcc ucgaaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaacaaua agcacuggcu gguccauaaa gaaugguucc acgacauccc acucccgugg     1260

cacgccggcg cagauacugg aacaccccau uggaacaaua aggaggcccu uguggaauuu     1320

aaagacgcuc acgcgaagcg ccagaccgua guugugcugg gcagccagga gggggcaguc     1380

cauaccgccu uggcuggagc ccuagaggca gaaauggaug gugccaaagg cagacugucc     1440

ucugggcacc ucaagugcag gcugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucauugugua cugcugccuu cacguuuaca aaaauuccug cagagacccu ccauggcacu     1560

gucaccgugg aaguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug     1620

gccguagaua ugcagacccu gacuccuguc ggccgcuuaa uaacagcaaa cccagugauc     1680

acggagagua ccgaaaacuc caaaaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaagaag auaacucacc acuggcauag aucuggauca     1800

acaauuggua aagccuuuga agcuaccguc aggggcgcca agcguauggc agugcucggg     1860

gacacagcuu gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc     1920

caccagaucu ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaca ccaaaaaugg uagcauuucc     2040

uugauguguc ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  3938
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3938
gggagaaagc uuaccaugag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug cuauggcagc cgagguuacg cggcgcgguu ccgcguacua uauguaccug      120

gaucgaaacg acgcuggcga agccauuucu uuccccacca cauuggggau gaauaagugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgagugcccu      240

augcuggaug aaggcgugga gccagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaaa aagggggaag cccguagaag caggcgggcu      360

gugacacuac cgucccauuc uacccgcaaa cuccaaacga gaucgcagac uuggcuggag      420

ucaagggagu acaccaagca ccuuauucgg guugaaaauu ggaucuuucg caaccccgga      480

uucgcacugg ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug      600

agcaauagag auuuuguaga ggggaugucc ggagguacuu ggguugacau uguguuagaa      660

cauggcgggu gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcagaggug aggaguuacu gcuaugaagc uagcauaucu      780

gauauggccu ccgacucacg gugucccaca caaggagagg cguaccuuga caagcagagc      840

gauacccagu auguaugcaa acguacuuug guggaccgcg gcugggguaa ugggugugga      900

cuguucggca agggcagucu agucacaugc gccaaauuug caugcucuaa gaaaaugacc      960

gggaagucca uucagccuga aaaccucgag uacagaauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaaa ccgacgagaa cagggcuaag     1080

guggagauca caccaaauuc accccgggcc gaagcuacuc uugggggauu cggcucccug     1140

gguuuggauu gugagccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu ccauaaggaa ugguuccacg acaucccacu cccguggcac     1260

gccggcgcag auacuggaac accccauugg aacaauaaag aggcccuugu ggaauuuaag     1320

gacgcucacg cgaaacgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau     1380

accgccuugg cuggagcccu agaggcagaa auggauggug ccaagggcag acuguccucu     1440

gggcaccuca aaugcaggcu gaagauggac aagcugcggc ugaaaggagu gagcuauuca     1500

uuguguacug cugccuucac guuuacaaag auuccugcag agacccucca uggcacuguc     1560

accguggaag uucaguacgc ggguacagac gggccaugca aagugcccgc ucaaauggcc     1620

guagauaugc agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca     1800

auugguaagg ccuuugaagc uaccgucagg ggcgccaaac guauggcagu gcucggggac     1860

acagcuuggg auuucggaag uguaggcggu gcccugaaua gccuugggaa gggcauccac     1920

cagaucuucg gagcggccuu uaaauccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cucuuggggg aguguuaaua uuccugucaa cggcaguuag ugccugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  3939
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3939
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga gguuacgagg cgggguuccg cguacuauau guaccuggau      120

cgcaacgacg cuggcgaagc cauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga gugcccuaug      240

cuggaugaag gcguggagcc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaagccc gacguagcag aagggcugug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaguca      420

agagaguaca ccaagcaccu uauuaggguu gaaaauugga ucuuucggaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaggg gauguccgga gguacuuggg uugacauugu guuagaacau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaggugaga aguuacugcu augaagcuag cauaucugau      780

auggccuccg acucaaggug ucccacacaa ggagaggcgu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugaaaa ccucgaguac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugaaaccg acgagaacag agcuaaggug     1080

gagaucacac caaauucccc cagggccgaa gcuacucuug ggggauucgg cucacugggu     1140

uuggauugug agccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggaaugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaagagg cccuugugga auuuaaggac     1320

gcucacgcga aacgacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga ggcagaaaug gauggugcca agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaagauu ccugcagaga cccuccaugg cacugucacc     1560

guggaaguuc aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag caaacccagu gaucacggag     1680

aguaccgaaa acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggccu uugaagcuac cgucagaggc gccaaaagga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuc uugggggagu guuaauauuc cugucaacgg caguuagugc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3940
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3940
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaggu uacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

aacgacgcug gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug      240

gaugaaggcg uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caaaaagggg gaagcccgcc gaagccguag agcugugaca      360

cuaccguccc auucuaccag gaaacuccaa acgcggucgc agacuuggcu ggagucacgc      420

gaguacacca agcaccuuau uagaguugaa aauuggaucu uuaggaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc ggugcaucgg cgugagcaau      600

cgcgauuuug uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga ggugcgaagu uacugcuaug aagcuagcau aucugauaug      780

gccuccgacu caagaugucc cacacaagga gaggcguacc uugacaagca gagcgauacc      840

caguauguau gcaaaaggac uuugguggac cggggcuggg guaaugggug uggacuguuc      900

ggcaagggca gucuagucac augcgccaaa uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugaaaaccu cgaguaccgu aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gaaaccgacg agaaccgcgc uaagguggag     1080

aucacaccaa auucccccag agccgaagcu acucuugggg gauucggcuc acuggguuug     1140

gauugugagc cuaggaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aaacacuggc ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu     1320

cacgcgaaac ggcagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaggc agaaauggau ggugccaagg gccgacuguc cucugggcac     1440

cucaaaugcc gccugaagau ggacaagcug agacugaaag gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug     1560

gaaguucagu acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggcagguua auaacagcaa acccagugau cacggagagu     1680

accgaaaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcauc ggucuggauc aacaauuggu     1800

aaggccuuug aagcuaccgu ccgcggcgcc aaaagaaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc     1920

uucggagcgg ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcucuug ggggaguguu aauauuccug ucaacggcag uuagugccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  3941
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3941
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccuuacaacu       60

gcuauggcag ccgagguuac gagaagggga uccgcguacu auauguaccu ggaucggaac      120

gacgcuggug aagccauuuc uuuccccacc acauugggca ugaauaagug cuauauacag      180

aucauggauu uagggcacau gugugacgca acuaugucau acgagugccc uaugcuggau      240

gaaggagugg agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uauggcacuu gucaucacaa aaagggugaa gcccgccgaa gccguagagc ugugacacua      360

ccgucccauu cuaccaggaa acuccaaacg cggucgcaga cuuggcugga gucacgcgag      420

uacaccaagc accuuauuag aguugaaaau uggaucuuua ggaaccccgg guucgcacug      480

gccgcugccg cgauugcaug guugcucgga agcaguacau cccagaaagu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucggu gcaucggcgu gagcaaucgc      600

gauuuuguag agggcauguc cgggggaacu uggguugaca uuguguuaga acaugguggc      660

ugugucaccg ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaggu gcgaaguuac ugcuaugaag cuagcauauc ugauauggcc      780

uccgacucaa gaugucccac acaaggggag gcguaccuug acaagcagag cgauacccag      840

uauguaugca aaaggacuuu gguggaccgg ggauggggca augguugugg gcuguucgga      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaaaaugac cggcaagucc      960

auucagccug aaaaccucga guaccguauc augcugagcg ugcacggguc gcaacacucu     1020

ggaaugauug uuaaugauac gggucaugaa accgacgaga accgcgcuaa gguggagauc     1080

acaccaaauu cccccagagc cgaagcuacu cuuggcgggu ucggaucacu gggcuuggau     1140

ugugagccua ggaccggucu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gacaucccac ucccguggca cgccggggca     1260

gauacuggca caccccauug gaacaauaaa gaggcccuug uggaauuuaa ggacgcucac     1320

gcgaaacggc agaccguagu ugugcuggga agccaggagg gcgcagucca uaccgccuug     1380

gcuggggccc uagaggcaga aauggaugga gccaaggguc gacuguccuc uggccaccuc     1440

aaaugccgcc ugaagaugga caaacuuaga cugaaggggg ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gagacccucc auggaacugu caccguggaa     1560

guucaguacg cgggcacaga cgguccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg gagguuaaua acagcaaacc cagugaucac ggagaguacc     1680

gaaaacucca agaugaugcu ggagcuagau cccccguucg gcgacagcua cauuguuauc     1740

ggagugggcg agaaaaagau aacucaccac uggcaucggu cugggucaac aauuggaaag     1800

gccuuugaag cuaccguccg cggugccaaa agaauggcag ugcucggcga cacagcuugg     1860

gauuucggga guguaggagg cgcccugaau agccuuggua aggggaucca ccagaucuuc     1920

ggcgcggccu uuaaauccuu guucggaggc augucuuggu uuucgcaaau ucugaucggg     1980

acucuccuga uguggcuugg acugaacacc aagaauggca gcauuuccuu gaugugucuc     2040

gcucugggug ggguguuaau auuccuguca acggcaguua gugccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  3942
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3942
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu uacaacugcc       60

auggcugcag agguuacgag aaggggaucc gccuacuaua uguaccugga ucggaacgac      120

gcgggugaag cuauuucuuu ccccaccaca uugggcauga auaagugcua uauacagauc      180

auggauuuag ggcacaugug ugacgccacu augucauacg agugcccuau gcuggaugaa      240

ggaguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

ggcacuuguc aucacaaaaa gggugaagca cgccgaagcc guagagccgu gacacuaccg      360

ucccauucua ccaggaaacu ccaaacgcgg ucgcagacuu ggcuggaguc acgcgaguac      420

accaagcacc uuauuagagu ugaaaauugg aucuuuagga accccggguu cgcucuggca      480

gccgcugcca uugcgugguu gcucggaagc aguacauccc agaaagugau cuaucugguc      540

augauacugc ucaucgcacc cgccuacucu auucggugca ucggcgugag caaucgcgau      600

uuuguagagg gcauguccgg gggaacuugg guugacauug uguuagaaca ugguggcugu      660

gucaccguga uggcucagga uaagccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg ccgaggugcg aaguuacugc uaugaagcaa gcauaucuga uauggcuucc      780

gacucaagau gucccacaca aggggaggcc uaccuugaca agcagagcga uacccaguau      840

guaugcaaaa ggacuuuggu ggaccgggga uggggcaaug guugugggcu guucggaaag      900

ggcagucuag ucacaugcgc gaaauuugcc ugcucuaaga aaaugaccgg caaguccauu      960

cagccugaaa accucgagua ccguaucaug cugagcgugc acgggucgca acacucugga     1020

augauuguua augauacggg ucaugaaacc gacgagaacc gcgcaaaggu ggagaucaca     1080

ccaaauuccc ccagagcuga agccacucuu ggcggguucg gaucacuggg cuuggauugu     1140

gagccuagga ccggucucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc uggggccgau     1260

acuggcaccc cucauuggaa caauaaagag gcacuugugg aauuuaagga cgcccacgcu     1320

aaacggcaga caguaguugu gcugggaagc caggagggcg cgguccauac cgcauuggcc     1380

ggggcucuag aggccgaaau ggauggagca aagggucgac uguccucugg ccaccucaaa     1440

ugccgccuga agauggacaa acuuagacug aaggggguga gcuauucauu guguacugcc     1500

gcuuucacgu uuacaaagau uccugccgag acccuccaug gaacugucac cguggaaguu     1560

caguacgcag gcacagacgg uccaugcaaa gugcccgcgc aaauggcugu agauaugcag     1620

acccugacuc cugucgggag guuaauaaca gccaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggcg acagcuacau uguuaucgga     1740

gugggcgaga aaaagauaac ucaccacugg caucggucug ggucaacaau uggaaaggca     1800

uuugaagcca ccguccgcgg ugcuaaaaga auggccgugc ucggcgacac agcaugggau     1860

uucgggagug uaggaggcgc ucugaauagc cuugguaagg ggauccacca gaucuucggc     1920

gccgcguuua aauccuuguu cggaggcaug ucuugguuuu cgcaaauucu gaucgggacu     1980

cuccugaugu ggcuuggacu gaacaccaag aauggcagca uuuccuugau gugucucgcc     2040

cugggugggg uguuaauauu ccugucaacg gcuguuagug caugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  3943
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  3943
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3944
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3944
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagccg uuacgagaag gggauccgcg uacuauaugu accuggaccg gaacgaugcu      120

ggugaggcca uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcaacuaug ucauacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaggcccgc cgaagccgua gagcugugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggaaucacg cgaguacacc      420

aagcaccuua uuagaguuga gaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagaaggca uguccggggg aacuuggguu gauauugugu uagagcaugg uggcuguguc      660

accgugaugg cccaggacaa gccaacagug gauaucgaac uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaggcuagca uaucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaagcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugagaacc ucgaauaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugagaccgau gaaaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaggc uacucuuggc ggguucggau cacugggcuu ggacugugaa     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggagugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacacu     1260

ggcacacccc auuggaacaa uaaagaagcc cuuguggagu uuaaggaugc ucacgcgaaa     1320

cggcagaccg uaguugugcu gggaagccag gaaggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagagaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagaaacc cuccauggaa cugucaccgu ggagguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaggcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  3945
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3945
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccgc gguuacgagg cgggguuccg cuuacuauau guaccuggau      120

cgcaacgacg ccggcgaggc aauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgccacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaggcuc gacguagcag aagggcagug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaauca      420

agagaguaca ccaagcaccu uauuaggguu gagaauugga ucuuucggaa ccccggauuc      480

gcccuggcug ccgcggcaau ugccugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgcuccu gccuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcacaggau aagccaacag uggacaucga acuggucacc      720

acgacuguuu caaacauggc ugaggugaga aguuacugcu augaggccag cauaucugau      780

auggcguccg acucaaggug ucccacacaa ggagaagccu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgca aaauuugcuu gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgaaaacag agccaaggug     1080

gagaucacac caaauucccc cagggcugag gccacucuug ggggauucgg cucacugggu     1140

uuggauugug aaccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgca     1260

ggcgccgaua cuggaacacc ccauuggaac aauaaagaag cucuugugga guuuaaggac     1320

gcgcacgcaa aacgacagac cguaguugug cugggcagcc aggaaggggc cguccauacc     1380

gcuuuggccg gagcacuaga ggccgagaug gauggugcua agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugccg cauucacguu uacaaagauu ccugcggaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcugg uacagacggg ccaugcaaag ugcccgccca aauggcagua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag ccaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggaa cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggcuu uugaggccac cgucagaggc gcaaaaagga uggcugugcu cggggacaca     1860

gccugggauu ucggaagugu aggcggugcg cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag ccgcuuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcac uugggggagu guuaauauuc cugucaacgg ccguuagugc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  3946
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3946
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagguguuac ucgacguggc      120

ucagccuacu auauguaccu ggauagaaac gacgcugggg aggcaauuag uuuucccacg      180

acccuaggaa ugaauaaaug cuauauacaa aucauggauc ucggccauau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggugugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugggaccu gucaccauaa gaaaggagag      360

gcgaggcggu cucgcagagc ugugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg cuuugcccug gcagccgcug caauugccug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc uccugccuac      600

uccauuaggu gcaucggggu gucaaaucgg gauuucguag aaggaaugag ugguggcaca      660

uggguugaca uuguguugga gcacggggga ugugucacug ugauggcgca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggcagaggu gcguucuuac      780

ugcuaugagg ccuccauauc agauauggcu agcgacaguc gcugucccac ucagggcgaa      840

gccuaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

gguuggggga auggaugugg ccuguuuggc aaaggguccc uugucacuug cgcaaaguuc      960

gcuugcagca aaaagaugac aggaaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacgguuc ccagcauuca ggcaugauug uuaaugauac cgggcacgag     1080

acggacgaaa accgagccaa aguggagauc accccaaaua guccccgcgc ggaggccaca     1140

cucggaggcu uugguagccu gggguuagau ugugaaccua gaacuggccu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcaggagcu gauacaggca cuccccacug gaacaauaag     1320

gaagcccuag uggaguucaa agacgcucau gccaagaggc agaccguagu ugugcugggg     1380

ucucaggaag gagcagucca cacagcccuc gcuggugcgc uggaggcaga gauggauggc     1440

gccaaagggc ggcuuagcuc aggacaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggcg ugaguuauuc cuuauguacc gcugccuuua cuuucacgaa aauuccugca     1560

gaaacacugc acgguaccgu cacuguggag guucaauacg ccgggaccga cggcccaugc     1620

aaggugcccg cucagauggc cguagauaug cagacacuaa ccccugucgg aaggcucaua     1680

acugcaaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gcgacucaua cauuguuauc ggggugggag agaagaagau aacccaccau     1800

uggcaccgua gugguagcac uauuggcaaa gcguucgagg cuacaguccg aggggccaag     1860

cggauggcag uguugggaga caccgccugg gauuucggcu ccguaggugg ggcucucaau     1920

ucucugggca aaggaaucca ucaaaucuuu ggcgccgcau ucaagucgcu guuuggggga     1980

augagcuggu ucucccagau ucuuaucggc acacuguuga uguggcucgg ucugaacacu     2040

aaaaaugggu caauuaguuu aaugugucug gcucuaggag gcgugcucau auuucugucu     2100

accgccguua gcgcgugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3947
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  3947
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagccguuac ucgacguggu      120

ucagcuuacu auauguaccu ggauagaaac gacgcaggcg aggccauuag uuuucccacg      180

acccuaggga ugaauaaaug cuauauacaa aucauggauc ucggacauau gugugacgcg      240

acaaugagcu acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugguaccu gucaccauaa gaaaggggag      360

gcuaggcggu cucgcagagc cgugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg auuugcacug gccgcugcag ccauugcuug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc cccugcguac      600

uccauuaggu gcaucggcgu gucaaaucgg gauuucguag aagggaugag uggagguaca      660

uggguugaca uuguguugga gcacggcggg ugugucacug ugauggcaca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggccgaggu gcguucuuac      780

ugcuaugagg cuuccauauc agauauggcc agcgacaguc gcugucccac ucagggagaa      840

gcauaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

ggcuggggua augggugugg acuguuuggc aaaggcuccc uugucacuug cgcuaaguuc      960

gccugcagca aaaagaugac agggaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacggauc ccagcauuca gguaugauug uuaaugauac cggccacgag     1080

acggacgaaa accgagcgaa aguggagauc accccaaaua guccccgcgc cgaggcaaca     1140

cucgggggau uuggcagccu ggguuuagau ugugaaccua gaacugggcu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcuggcgcc gauacaggaa cuccccacug gaacaauaag     1320

gaagcucuag uggaguucaa agacgcccau gcaaagaggc agaccguagu ugugcugggc     1380

ucucaggaag gggccgucca cacagcucuc gcgggagcac uggaggccga gauggauggu     1440

gcuaaaggcc ggcuuagcuc agggcaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggag ugaguuauuc cuuauguacc gccgcauuua cuuucacgaa aauuccugcc     1560

gaaacacugc acggcaccgu cacuguggag guucaauacg cugguaccga cgggccaugc     1620

aaggugcccg cccagauggc aguagauaug cagacacuaa ccccugucgg caggcucaua     1680

acugcgaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gagacucaua cauuguuauc ggcguggggg agaagaagau aacccaccau     1800

uggcaccgua guggaagcac uauugguaag gcuuucgagg ccacaguccg gggcgcaaaa     1860

cgaauggccg uguuggggga caccgcuugg gauuucggau ccguaggcgg ugcccucaau     1920

ucucugggga aaggcaucca ucaaaucuuu ggagcagcuu ucaagucgcu guuuggcggg     1980

augagcuggu ucucccagau ucuuaucgga acacuguuga uguggcucgg ccugaacacu     2040

aaaaaugguu caauuaguuu aaugugucug gcccuagggg gagugcucau auuucugucu     2100

accgcgguua gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  3948
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 1 opt

<400>  3948
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3949
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 1 opt

<400>  3949
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacacu      240

ugugacgcca ccaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac aaccaguacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacccuac cgucccauuc uacgaggaag      420

cuccaaacuc ggucgcagac cuggcuggag ucacgcgaau acacaaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacuuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcacag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccacc      840

caaggagaag ccuaccuuga caaacagagc gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugaccugc      960

gcaaaguuug cuugcucuaa gaaaaugacg gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cagacgagaa ucgagccaaa gucgaaauca cuccaaacuc accccgcgcu     1140

gaggccaccc uugggggauu cggcucccug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagug aucuguauua cuuaacuaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auaccggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau acugcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacgg ccgcauucac auuuaccaaa     1560

auaccugcgg aaacucucca uggcaccgug acaguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacucugac accugugggc     1680

agguuaauca cggccaaccc aguuauuacc gaaaguacug aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acacaccacu ggcaucguuc uggaucaacc aucgguaagg cuuuugaggc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acugccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaaugcaa agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa cggcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3950
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 1 opt

<400>  3950
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugg cuacgaaacc     1080

gaugaggacc gagcaaaggu cgaggugaca ccuaauucac cacgcgcuga agccacucuu     1140

gggggauucg gcagucuggg uuuggauugu gagcccagaa ccgggcucga cuuuagcgau     1200

cuguauuacu uaacaaugaa caauaaacac uggcugguac auaaggaaug guuccacgac     1260

auaccucucc cauggcacgc uggcgccgau acuggaaccc cgcauuggaa caauaaagag     1320

gcacuuguug aauuuaagga cgcccacgcu aagaggcaga caguggucgu gcugggcucc     1380

caggaggggg cgguccauac cgcauuggcc ggagcucuag aggccgaaau ggauggugca     1440

aaaggccggc uguucucugg gcaccucaag ugccgccuga aaauggacaa acuuagacug     1500

aagggaguga gcuauucauu guguacugcc gcuuuuacgu ucacaaaggu ucccgccgag     1560

acccuccaug gcacugugac cguagaaguc caguacgcag guacagacgg gccuugcaag     1620

aucccagugc aaauggcggu ugauaugcag acccugacuc ccgugggcag guuaauuaca     1680

gcuaacccug ucaucacgga gaguaccgaa aauuccaaaa ugaugcugga gcuagaccca     1740

cccuuuggag auagcuacau agugauuggc guaggggaca agaaaaucac ucaccacugg     1800

caucguucug gaucaacaau cgguaaggcc uucgaggcaa ccgugcgggg cgccaaacga     1860

auggcuguuc ucggggauac agccugggac uucggaagug ucggcggugu guuuaacagc     1920

cuggggaagg gcauucacca gaucuucgga gcagcuuuua agucccuuuu cggcgggaug     1980

ucuugguuuu cgcaaauuuu gauaggaacu cugcucgugu ggcugggccu uaacaccaaa     2040

aaugguagca ucucccugac guguuuggcc cucgggggag ucaugauuuu ccugucaacc     2100

gcgguuagug ccgauguggg cugcucugug gacuuuagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3951
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 1 opt

<400>  3951
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3952
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 1 opt

<400>  3952
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugu caaugauacc     1080

ggcuacgaaa cagacgagaa ccgagcaaag guggagguaa cuccuaauuc accacgcgcu     1140

gaagccaccc uugggggauu cggcagucug gguuuggauu gugagcccag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaaac acuggcuggu ucauaaggaa     1260

ugguuccacg acauaccucu cccauggcac gcuggcgccg auaccggaac accgcauugg     1320

aacaauaaag aggcacuugu ggaauuuaag gacgcccacg cuaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcggugcau acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggcaa gcuguucucu gggcaccuca aaugccggcu gaagauggac     1500

aaacuucgcc ugaagggagu uagcuauuca uuguguacgg ccgcuuuuac auucaccaag     1560

gugcccgccg agacucucca uggcaccgua acagucgaag ugcaguacgc agguaccgac     1620

gggccuugca aaaucccagu ucaaauggcg guggauaugc agacucugac acccgucggc     1680

agauuaauua cggcuaaccc ugugaucacc gagaguacug aaaacuccaa gaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auaguaauug gcguggggga caaaaagauc     1800

acacaccacu ggcauagguc uggaucaacc aucgguaaag ccuucgaggc aacaguucgu     1860

ggcgccaagc ggauggcugu ccucggggau acugccuggg acuucggaag ugugggcggu     1920

guguuuaaua gccuggggaa gggcauucac cagaucuucg gagcagcuuu uaaaucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugauaggaa cccugcucgu cuggcugggc     2040

cuuaacacga agaaugguag caucucccug accuguuugg cccucggggg aguuaugauu     2100

uuccugucaa cagcggugag ugccgaugug ggcugcucug uagacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3953
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 1 opt

<400>  3953
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucacacugg uggcugucgc caucgcgugg      540

uugcucggca guuccacuuc ucagaaggug auuuaccugg uaaugauccu gcucauugca      600

cccgccuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguaccu gggucgacgu gguguuagaa cacggcgggu gugucacagu uauggcucag      720

gauaaaccua ccguggacau ugagcuggua acgacuacag ugaguaacau ggccgagguc      780

cguagcuacu gcuacgaagc aucuaucucc gauauggcuu cagacagccg cuguccaacc      840

caaggagagg ccuaucuuga caagcagagu gauacucagu acgugugcaa aagaacauug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugaccugc      960

gcgaaguuua cgugcuccaa aaagaugacc gggaaaagca uucagcccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu caacauuccg guaugaucgu gaaugaugaa     1080

aaccgagcca agguagaggu uacaccuaau ucaccacgcg cagaggcuac ucuuggcggg     1140

uucggaaguc ugggcuugga cugugaaccc agaaccgguc ucgauuuuag cgaccuguac     1200

uauuuaacaa ugaacaauaa acacuggcug gugcauaagg agugguucca cgauauuccu     1260

cucccauggc acgccggggc ugacacuggc accccgcauu ggaacaauaa agaagcccuu     1320

gucgaguuua aggaugcaca cgccaagagg cagacagugg ucgugcuggg aucccaggaa     1380

ggcgcuguuc auaccgcguu ggcaggggcc cuagaggcug agauggacgg agccaaaggu     1440

cggcuguucu cuggccaccu caagugccgc cugaaaaugg auaaacuuag acugaagggg     1500

gugagcuacu cauuguguac ugcagccuuu acguucacaa agguacccgc ugaaacccuc     1560

cauggaacug ucaccgugga gguucaguau gccggcacag acgguccuug caaggugcca     1620

gcacaaaugg cggucgacau gcagacccug acucccgugg ggagguuaau aacagcuaac     1680

ccuguaauca cggaaaguac cgagaacucc aaaaugaugc uggaacuaga uccacccuuu     1740

ggcgacagcu acauugugau cggaguuggc gauaagaaaa uaacucacca cuggcaucgu     1800

ucugggucaa caauuggaaa ggccuucgag gcaaccgucc ggggugccaa acgaauggcu     1860

gugcucggcg acacagccug ggauuucggg agugugggag gcgucuuuaa uagccugggu     1920

aaggggaucc accagaucuu cggcgcagcu uuuaaguccc uuuucggagg caugucuugg     1980

uuuucgcaaa uuuugaucgg gacucugcuc guuuggcugg gacuuaacac caaaaauggc     2040

agcauuuccc ugacguguuu ggcccucggu ggggugauga uauuccuguc aaccgcggug     2100

agugccgacg uaggaugcuc ugucgauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  3954
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 1 opt

<400>  3954
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccggaag      360

aaaggggaag cacgcagauc caggcgggcc gucacacuac caucucauuc gacccgcaag      420

cuccaaacgc gaucacagac uuggcuggag agcagagagu auaccaagca ccuuauaaaa      480

guggaaaauu ggaucuuuag gaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauacggug cauuggcgua uccaaucgug auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cauggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cgcagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagcag auguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aaggacuuug      900

guggaccggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucgaauca ugcugucggu gcacggaucu caacacuccg guaugauugu caaugauacc     1080

ggcuacgaaa cagacgagaa ccgcgcaaag guggagguaa cuccuaauuc accaagagcu     1140

gaagccaccc uugggggauu cggcagucug gguuuggauu gugagcccag gacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaaac auuggcuggu ucacaaggaa     1260

ugguuccaug acauaccucu cccauggcac gcuggcgccg auaccggaac accgcacugg     1320

aacaauaaag aggcacuugu ggaauuuaag gacgcccaug cuaagcggca gaccgucgug     1380

guccugggcu cccaggaggg ggcggugcac acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggcaa gcuguucucu gggcaucuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu uagcuauuca uuguguacgg ccgcuuuuac auucaccaag     1560

gugcccgccg agacucucca cggcaccgua acagucgaag ugcaguacgc agguaccgac     1620

gggccuugca aaaucccagu ucaaauggcg guggauaugc agacucugac acccgucggc     1680

agguuaauua cggcuaaccc ugugaucacc gagaguacug aaaacuccaa gaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auaguaauug gcguggggga caaaaagauc     1800

acacaucacu ggcaccguuc uggaucaacc aucgguaaag ccuucgaggc aacaguucgg     1860

ggcgccaagc gaauggcugu ccucggggau acugccuggg acuucggaag ugugggcggu     1920

guguuuaaua gccuggggaa gggcauucau cagaucuucg gagcagcuuu uaaaucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugauaggaa cccugcucgu cuggcugggc     2040

cuuaacacga agaaugguag caucucccug accuguuugg cccucggggg aguuaugauu     2100

uuccugucaa cagcggugag ugccgaugug ggcugcucug uagacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3955
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 1 opt

<400>  3955
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugaccgu caaugauauu     1080

ggcuacgaaa cagacgagaa ccgagcaaag guggagguaa cuccuaauuc accacgcgcu     1140

gaagccaccc uugggggauu cggcagucug gguuuggauu gugagcccag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaaac acuggcuggu ucauaaggaa     1260

ugguuccacg acauaccucu cccauggcac gcuggcgccg auaccggaac accgcauugg     1320

aacaauaaag aggcacuugu ggaauuuaag gacgcccacg cuaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcggugcau acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggcaa gcuguucucu gggcaccuca aaugccggcu gaagauggac     1500

aaacuucgcc ugaagggagu uagcuauuca uuguguacgg ccgcuuuuac auucaccaag     1560

gugcccgccg agacucucca uggcaccgua acagucgaag ugcaguacgc agguaccgac     1620

gggccuugca aaaucccagu ucaaauggcg guggauaugc agacucugac acccgucggc     1680

agauuaauua cggcuaaccc ugugaucacc gagaguacug aaaacuccaa gaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auaguaauug gcguggggga caaaaagauc     1800

acacaccacu ggcauagguc uggaucaacc aucgguaaag ccuucgaggc aacaguucgu     1860

ggcgccaagc ggauggcugu ccucggggau acugccuggg acuucggaag ugugggcggu     1920

guguuuaaua gccuggggaa gggcauucac cagaucuucg gagcagcuuu uaaaucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugauaggaa cccugcucgu cuggcugggc     2040

cuuaacacga agaaugguag caucucccug accuguuugg cccucggggg aguuaugauu     2100

uuccugucaa cagcggugag ugccgaugug ggcugcucug uagacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3956
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 1 opt

<400>  3956
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugg cuacgaaacc     1080

gaugagaauc gagcaaaggu cgaggugaca ccuaacucac cacgcgcuga agccacucuu     1140

gggggauucg gcagucuggg uuuggacugu gagcccagaa ccgggcucga uuuuagcgac     1200

cuguauuacu uaacaaugaa uaacaaacac uggcugguac auaaggaaug guuccacgau     1260

auaccucucc cauggcacgc uggcgccgac acuggaaccc cgcauuggaa uaacaaagag     1320

gcacuuguug aauuuaagga ugcccacgcu aagaggcaga caguggucgu gcugggcucc     1380

caggaggggg cgguccauac cgcauuggcc ggagcucuag aggccgaaau ggacggugca     1440

aaaggcaagc uguucucugg gcaccucaaa ugccggcuga agauggauaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcuuuuacgu ucacaaaggu ucccgccgag     1560

acccuccaug gcacugugac cguagaaguc caguacgcag guacagacgg gccuugcaaa     1620

aucccagugc aaauggcggu ugacaugcag acccugacuc ccgugggcag auuaauuaca     1680

gcuaauccug ucaucacgga gaguaccgaa aacuccaaga ugaugcugga gcuagaucca     1740

cccuuuggag acagcuacau agugauuggc guaggggaua aaaagaucac ucaccacugg     1800

cauaggucug gaucaacaau cgguaaagcc uucgaggcaa ccgugcgugg cgccaagcgg     1860

auggcuguuc ucggggacac agccugggau uucggaagug ucggcggugu guuuaacagc     1920

cuggggaagg gcauucacca gaucuucgga gcagcuuuua aaucccuuuu cggcgggaug     1980

ucuugguuuu cgcaaauuuu gauaggaacu cugcucgugu ggcugggccu uaauaccaag     2040

aacgguagca ucucccugac guguuuggcc cucgggggag ucaugauuuu ccugucaacc     2100

gcgguuagug ccgacguggg cugcucugug gauuuuagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  3957
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 1 opt

<400>  3957
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggag aggcauccac cagauuuucg gagccgcuuu uaaauccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3958
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 1 opt

<400>  3958
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacgcaagcg uggggauagu cggacugcuc cuuaccacag ccauggcggc ugaaguuacu      120

cgacgugguu ccgccuacua uauguaccug gauagaaaug acgcaggcga ggccaucucu      180

uuccccacga ccuuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcua caaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac uaccaguaca uggguggucu augguaccug ucaucacaag      360

aaaggggagg caaggcggag ccgcagagcc gugacucuac cgucccauuc uacaaggaag      420

cuccaaaccc ggucgcagac guggcuggag ucacgcgaau acacuaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcucugg ccgcggcagc cauagcuugg      540

uugcucggca gcaguaccuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcauacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacau ggguugacgu ggucuuagag cauggcgggu gugugacugu gauggcucag      720

gauaagccaa ccgucgacau agagcugguu acaaccacgg ugucaaauau ggccgaagua      780

cguaguuacu gcuaugaggc gagcauuucu gauauggccu ccgacucacg cugucccacu      840

caaggagaag cauaccuuga caaacagagc gauacacagu augugugcaa gagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacuugc      960

gcuaaguuug ccugcucuaa gaaaaugaca gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cggacgagaa ucgagcuaaa gucgaaauca ccccaaacuc accccgcgcc     1140

gaggcaacac uugggggauu cggcucccug gguuuggauu gugaaccuag aacugggcuc     1200

gacuuuagug aucuguauua cuuaaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcug auacaggaac uccccauugg     1320

aauaacaagg aagcgcuugu agaguuuaaa gacgcacacg ccaagaggca gaccguugug     1380

guccugggca gccaggaggg ggcugugcau acagccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguaccg cagcguucac uuuuacgaaa     1560

auaccugcug aaacacucca uggcaccgug acuguugagg ugcaguacgc cgguaccgac     1620

gggccaugca agguacccgc acaaauggcc gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugcuaaccc aguuauuaca gaaaguacgg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaaag ccuuugaggc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggaag ugugggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa ccgcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3959
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 1 opt

<400>  3959
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga cuggccuugg cugccgcggc aauagccugg      540

cuccugggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu cuuaaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuucguaga agggaugucc      660

ggagguacuu ggguugacgu gguccuggag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcuuguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuacuugga caaacagagc gauacccagu augugugcaa gagaacucug      900

gucgacaggg gcugggguaa cgggugugga cuauuuggca aaggcagucu cgugacaugc      960

gcaaaguucg cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucuggaa     1020

uaccggauua ugcuuagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc ugggaggguu uggcuccuug ggucucgauu gugaaccuag aaccgggcug     1200

gacuucagug auuuauauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuucacg acauuccacu uccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuggu agaguucaaa gacgcgcacg caaagaggca gaccguugug     1380

gucuugggca gccaggaggg ggccgugcau accgcucuag ccggagcacu ggaagccgag     1440

auggauggug cuaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggac     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug ccgcauuuac guucacaaaa     1560

auaccugcgg aaacccugca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agaccuuaac uccugugggc     1680

aggcugauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc ccccguuugg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuucgaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acugggggac acagccuggg auuuuggaag ugugggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagauuuucg gagccgcuuu caagucccug     1980

uuuggcggga ugucuugguu cucgcaaauc cucauuggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauaucccuc augugucugg cauuaggggg aguucugauc     2100

uuucuaucaa cggccgucag ugcugacgug ggcugcucug uggauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3960
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 1 opt

<400>  3960
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu gguaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccau cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3961
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 1 opt

<400>  3961
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaaauu      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3962
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 1 opt

<400>  3962
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacuggc ccaaauggca gucgauaugc agaccuuaac ucccgugggc     1680

aggcugauca cagccaaccc uguuauuacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgacc cacccuucgg agauagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acugggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagauuuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uucgcaaauc cucauuggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauaucccuc augugucugg cauuaggggg aguucugauc     2100

uuccuaucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3963
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 1 opt

<400>  3963
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu acccuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac aaccaguacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacccuac cgucccauuc uacgaggaag      420

cuccaaacuc ggucgcagac cuggcuggag ucacgcgaau acacaaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacuuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcacag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccacc      840

caaggagaag ccuaccuuga caaacagagc gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugaccugc      960

gcaaaguuug cuugcucuaa gaaaaugacg gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cagacgagaa ucgagccaaa gucgaaauca cuccaaacuc accccgcgcu     1140

gaggccaccc uugggggauu cggcucccug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagug aucuguauua cuuaacuaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auaccggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau acugcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacgg ccgcauucac auuuaccaaa     1560

auaccugcgg aaacucucca uggcaccgug acaguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacucugac accugugggc     1680

agguuaauca cggccaaccc aguuauuacc gaaaguacug aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acacaccacu ggcaucguuc uggaucaacc aucgguaagg cuuuugaggc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acugccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaauacga agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa ccgccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3964
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 1 opt

<400>  3964
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggucgugag ugccgacgug ggcugcucug ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3965
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 1 opt

<400>  3965
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaagugcu uggguggucu augguaccug ucaucacaag      360

aaaggggagg caaggcggag ccgcagagcc gugacucuac cgucccauuc uacaaggaag      420

cuccaaaccc ggccccagac guggcuggag ucgcgcgaau acacuaagca ccuuauucga      480

guugagaacu ggaucuuuag aaauccugga uucgcucugg ccgcggcagc cauagcuugg      540

uugcucggcu caagcaccag ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccagcauacu ccauuaggug caucggcgug ucuaaccggg auuuuguaga agggaugagc      660

ggagguacau ggguugacgu ggucuuagag cauggcgggu gugugacugu gauggcucag      720

gauaagccca ccgucgacau agagcugguu acaaccacgg uguccaauau ggccgaagua      780

cguucauacu gcuaugaggc gaguauuagc gauauggccu cugacucccg cuguccuacu      840

caaggagaag cauaccuuga caaacaguca gauacacagu augugugcaa gagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagccu agugacuugc      960

gcuaaguuug ccugcaguaa gaaaaugaca gggaagucua uccagccaga gaaucucgaa     1020

uaccggauua ugcuguccgu ucacggaagc caacacucgg guaugaucgu gaacgauacc     1080

ggccaugaga cggacgagaa ucgagcuaaa gucgaaauca cccccaacuc uccucgcgcc     1140

gaggcaacac uugggggauu cggcucccug gguuuggauu gugaaccaag aacugggcuc     1200

gacuuuucag aucuguauua cuuaaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccgcu ccccuggcac gccggcgcug auacaggaac uccucauugg     1320

aauaacaagg aagcgcuugu agaguuuaaa gacgcacacg ccaagaggca gaccguugug     1380

guccugggca gucaggaggg ggcugugcau acagccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguaccg cagcguucac uuuuacgaaa     1560

auaccagcug aaacacucca uggcaccgug acuguugagg ugcaguacgc cgguaccgac     1620

gggcccugca agguaccugc acaaauggcc gucgauaugc agacacugac cccagugggc     1680

agguuaauca cugcuaaccc cguuauuaca gaaucaacgg agaauaguaa gaugaugcug     1740

gagcuagacc cgccuuucgg agauuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguag cggaucuacu aucgguaaag ccuuugaagc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggauc agugggcggu     1920

gcccugaaca gucuugggaa aggcauccac cagauuuucg gagcugcauu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguuc gaucagcuug augugucucg ccuuaggggg aguucugaua     2100

uuccugucca ccgcugucuc agccgacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3966
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 1 opt

<400>  3966
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc caaaaaggug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaaaccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

cagggagaag ccuaccuuga caagcaaagc gauacccagu augugugcaa aagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca agggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa aaagaugacc gggaaaucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg cagcacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaag gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaaac acuggcuggu gcauaaggag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaaag aagcucuugu agaguuuaag gacgcgcacg caaagaggca aaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaaaggccg gcuguccucu gggcaccuca agugccgccu gaaaauggac     1500

aagcugagac ugaaaggagu cagcuauuca uuguguacug ccgcauucac guuuacaaag     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccagauggca gucgauaugc aaacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa aaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaagaaaauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa gggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcagauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca aaaacgguag cauauccuug augugucucg cacuuggggg aguuuuaauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3967
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 1 opt

<400>  3967
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacaag gcagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu ccauucggug caucggcgug ucuaaccgug auuuuguaga agggaugagc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug uguccaauau ggcugaagua      780

cgcucauacu gcuaugaggc caguauuagc gauauggcgu cugacuccag augucccaca      840

caaggagaag ccuaccuuga caaacaguca gauacccagu augugugcaa gaggacuuug      900

gucgaccggg gcugggguaa cgggugugga cuguucggca aaggcagccu agugacaugc      960

gcaaaguuug cuugcaguaa gaaaaugacc gggaagucua uccagccuga gaaucucgaa     1020

uaccgaauua ugcuguccgu ucacggaagc caacacucgg guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgcgccaaa gucgaaauca caccaaacuc ucccagagcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag gaccgggcuc     1200

gacuuuucag aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagcggca gaccguugug     1380

guccugggca gucaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg ccugagcucc gggcaccuca aaugcagacu gaagauggac     1500

aaacuuaggc ugaagggagu cucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

cguuuaauca cagccaaccc aguuauuacg gaaucaaccg agaauaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucggag cggaucuaca aucgguaagg cuuuugaagc caccgugcga     1860

ggcgcaaaaa gaauggcugu acucggggac acagccuggg auuucggauc agugggcggu     1920

gcgcugaaca gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguuc gaucagcuug augugucucg cauuaggggg aguucugaua     2100

uuccugucca cggccgucuc agcugacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3968
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 1 opt

<400>  3968
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcagcgcu       60

gacaccuccg ugggcauagu cgggcugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacguggau cugcuuacua uauguaccug gauagaaaug acgccgguga ggcaaucuca      180

uuccccacca cauugggcau gaacaaaugc uauauucaga ucauggauuu agggcacaug      240

ugugacgcca cuaugaguua cgaaugcccu augcuggaug agggagugga accagacgau      300

guagacuguu ggugcaauac cacaagcacc uggguggucu auggcacuug ucaucacaag      360

aaaggugagg cuaggcgguc ccgcagagca gugacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccggg uucgcccugg cugccgcggc aauagccugg      540

uugcucggaa guuccacauc ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuaca gcauuaggug caucggcgug uccaaccggg auuuuguaga aggcauguca      660

gggggaacuu ggguugacgu ggucuuagag caugguggcu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugaguaauau ggcugaagua      780

cguagcuacu gcuaugaggc cucuauuucc gauauggcgu cagacagccg cugucccaca      840

caaggggaag ccuaccuuga caaacagagu gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gauggggcaa cgguuguggg cuguucggaa aaggcucucu agugacaugc      960

gcaaaguuug cuugcuccaa gaaaaugacc ggcaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucggu ucacgggucu caacacuccg gaaugaucgu gaacgauacg     1080

ggucaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uuggcggguu cggaagucug ggcuuggauu gugaaccuag aaccggucuc     1200

gacuuuagcg aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggggccg auacuggcac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggau cccaggaggg cgccgugcau accgcuuugg ccggggcacu agaagccgag     1440

auggauggag cuaagggucg gcugucuagc ggccaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggggu cucauauagu uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggaacugug accguugagg ugcaguacgc uggcacagac     1620

gguccaugca agguacccgc ccaaauggca gucgauaccc agacucugac accugugggg     1680

agguuaauca cggccaaccc aguuauuacc gaauccacug agaauagcaa gaugaugcug     1740

gagcuagauc ccccguucgg cgacucuuac aucgugauag gagucggcga gaaaaagauu     1800

acacaccacu ggcaucguuc agggaguacc aucggaaagg cuuuugaagc cacagugcgg     1860

ggugcaaaac gaauggcugu acucggcgac acugccuggg auuucgggag cgugggaggc     1920

gcgcugaacu cccuugguaa agggauccac cagauuuucg gcgccgcuuu uaagucuuug     1980

uucggaggca ugucgugguu uagccaaauc cugauuggga cccuccugau guggcuugga     2040

cugaauacga agaacggcuc cauaucauug augugucucg cacugggugg gguuuuaauc     2100

uuccugagua ccgccgucuc ugcugacgug ggaugcagcg uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3969
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 1 opt

<400>  3969
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccaaug uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacguggua gcgcuuacua uauguaccug gauagaaacg acgccggcga ggcaaucucc      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucuua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac cacaucaacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ucgcagagca gugacacuac cgagccauuc caccaggaag      420

cuccaaacgc ggucucagac uuggcuggag ucgcgcgaau acaccaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggcu caagcacaag ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu ccauuaggug caucggcgug ucuaaucggg auuuuguaga agggaugagc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug uguccaacau ggcugaagua      780

cguucauacu gcuaugaggc caguauuagc gauauggcgu cugacucccg cugucccaca      840

caaggagaag ccuaccuuga caaacaguca gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcagccu agugacaugc      960

gcaaaguuug cuugcaguaa gaaaaugacc gggaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu ucacggaagc caacacucgg guaugaucgu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagccaaa gucgaaauca caccaaauuc uccccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuucag aucuguauua cuuaacaaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aacaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gucaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaucc aguuauuacg gaaucaaccg agaacaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguag cggaucuaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcacgaa gaauggcugu acucggggac acagccuggg auuucggauc agugggcggu     1920

gcgcugaaua gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaacacca aaaaugguuc gaucagcuug augugucucg cauuaggggg aguucugaua     2100

uuccugucca cggccgucuc agcugacgug ggcugcagug uggauuuuuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3970
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 1 opt

<400>  3970
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gaaaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggagguuacg      120

cgacgugguu ccgcuuacua uauguaccug gacagaaaug augccggcga agcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggacuu aggacacaug      240

ugugaugcca cuaugucaua cgagugcccu augcuggacg aaggcgugga gccagaugac      300

guagauuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggaa ucacgcgagu acaccaagca ccuuauucga      480

guugaaaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg acuuuguaga ggggaugucc      660

ggagguacuu ggguugaugu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gacaagccaa cagucgauau agaacugguu accacgacug ugucaaauau ggcugaggua      780

cguaguuacu gcuaugaagc cagcauuucu gacauggcgu ccgauucacg cugucccaca      840

caaggagagg ccuaccuuga caaacagagc gacacccagu augugugcaa gagaacuuug      900

gucgauaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga aaaucucgag     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgacacg     1080

ggccaugaga ccgaugaaaa ucgagccaaa gucgagauca caccaaacuc accccgcgcu     1140

gaagccacuc uugggggauu cggcucccug gguuuggacu gugagccuag aaccgggcuc     1200

gauuuuagug accuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagaa     1260

ugguuccacg auauuccacu cccguggcac gcaggcgccg acacuggaac accccauugg     1320

aauaacaagg aggcucuugu agaguuuaaa gaugcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaagg ggccgugcau accgcuuugg ccggagcacu agaggccgaa     1440

auggacggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggau     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg agacccucca uggcacugug accguugaag ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgacaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gagaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggau acagccuggg acuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugaugug ggcugcucug uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3971
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 1 opt

<400>  3971
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuuuaaggug cauuggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu gguccuggag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau cgagcuuguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauaucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuacuugga caaacagagc gauacccagu augugugcaa gagaacucug      900

gucgacaggg gcugggguaa cgggugugga cuauucggca aaggcagucu cgugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucuggaa     1020

uaccggauca ugcuuagcgu ucacggaucg caacacucug guaugauugu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc ugggaggguu cggcuccuug ggucucgauu gugaaccuag aaccgggcug     1200

gacuuuagug auuuauauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acaucccacu uccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuggu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

gucuugggca gccaggaggg ggccgugcau accgcucuag ccggagcacu ggaagccgag     1440

auggauggug cuaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggac     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug ccgcauucac guuuacaaaa     1560

auuccugcgg aaacccugca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agaccuuaac uccugugggc     1680

aggcugauaa cagccaaccc aguuaucacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc ccccguucgg agacagcuac auugugaucg gcgucgggga gaaaaagaua     1800

acucaccacu ggcaucguuc uggaucaaca auugguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acugggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uucgcaaauu cucaucggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauuucccuc augugucugg cauuaggggg aguucugaua     2100

uuccuaucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3972
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 1 opt

<400>  3972
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccaaug uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacguggua gcgcuuacua uauguaccug gauagaaacg acgccggcga ggcaaucucc      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucuua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac cacaucaacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ucgcagagca gugacacuac cgagccauuc caccaggaag      420

cuccaaacgc ggucucagac uuggcuggag ucgcgcgaau acaccaagca ccuuauucga      480

guugagaauu ggauguuuag aaaccccgga uucgcccugg cugccgcggc aaucgccugg      540

uugcucggcu caagcacaag ucagaaagug auauaucugg ucaugauccu gcucauugcu      600

ccugccuacu ccaucaggug cauuggcgug ucuaaucggg auuuuguaga agggaugagc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau cgagcugguu accacgacug uguccaacau ggcugaagua      780

cguucauacu gcuaugaggc caguauaagc gauauggcgu cugacucccg cugucccaca      840

caaggagaag ccuaccuuga caaacaguca gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcagccu agugacaugc      960

gcaaaguuug cuugcaguaa gaaaaugacc gggaagucua uucagccuga gaaccucgaa     1020

uaccggauca ugcuguccgu ucacggaagc caacacucgg guaugauugu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagccaaa gucgaaauca caccaaauuc uccccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuucag aucuguauua cuuaacaaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acaucccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aacaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gucaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auuccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauaa cagccaaucc aguuaucacg gaaucaaccg agaacaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac auugugaucg gcgucgggga gaaaaagaua     1800

acucaccacu ggcaucguag cggaucuaca auugguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggauc agugggcggu     1920

gcgcugaaua gucuugggaa aggcauccac cagaucuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cucuccugau guggcuuggc     2040

cugaacacca agaaugguuc gauuagcuug augugucucg cauuaggggg aguucugaua     2100

uuccugucca cggccgucuc agcugacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3973
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 1 opt

<400>  3973
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggauccugag aaaucccgga uuugccuugg cugccgcggc aauagccugg      540

cuccugggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu cuuaaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuucguaga agggaugucc      660

ggagguacuu ggguugacgu gguccuggag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcuuguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuacuugga caaacagagc gauacccagu augugugcaa gagaacucug      900

gucgacaggg gcugggguaa cgggugugga cuauuuggca aaggcagucu cgugacaugc      960

gcaaaguucg cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucuggaa     1020

uaccggauua ugcuuagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc ugggaggguu uggcuccuug ggucucgauu gugaaccuag aaccgggcug     1200

gacuucagug auuuauauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuucacg acauuccacu uccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuggu agaguucaaa gacgcgcacg caaagaggca gaccguugug     1380

gucuugggca gccaggaggg ggccgugcau accgcucuag ccggagcacu ggaagccgag     1440

auggauggug cuaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggac     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug ccgcauuuac guucacaaaa     1560

auaccugcgg aaacccugca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agaccuuaac uccugugggc     1680

aggcugauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc ccccguuugg agacagcuac aucgugauag gcauugggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuucgaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcugggggac acagccuggg auuuuggaag uguaggcggu     1920

gcgcuuaaca gcuuggggaa aggcauucac cagaucuucg gagccgcuuu caagucccug     1980

uuuggcggga ugucuugguu cucgcaaauu cucauaggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag caucucccuc augugucugg cauuaggggg agugcugauu     2100

uuucuaucaa cggccguuag ugcugacguc ggcugcucug uggauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3974
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 1 opt

<400>  3974
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu ucaaccacgg ugaguaauau ggcugaagua      780

cguagcuacu gcuaugaggc cacuauuucu gauaucgcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccgccguu     1380

gugcugggca gccaggaggg ggcuguccau accgccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug cagcguucac guuuacaaaa     1560

aucccugcug aaacccucca uggcacuguc accguggagg uucaguacgc cgguacagac     1620

gggccaugca aggugcccgc acaaauggcc guagauaugc agacccugac uccugucggc     1680

agguuaauua cagcuaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaaag ccuuugaggc aaccguccgg     1860

ggcgcuaagc gaauggccgu gcucggggac acagcguggg auuucggaag uguaggcggu     1920

gcccugaaca gccuugggaa aggcauucac cagaucuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg ccuuaggggg agugcugauu     2100

uuccugucaa cggcuguuag ugccgacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3975
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 1 opt

<400>  3975
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacag cccccgcgcu     1140

gaggccacuc uugggggauu cggcucacug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca cccaggaggg ggccgugcau acugcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguacgg ccgcauucac auuuaccaaa     1560

auaccugcgg aaacucucca uggcaccgug acaguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacucugac accugugggc     1680

agguuaauca cggccaaccc aguuauuacc gaaucaacug agaauaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acacaccacu ggcaucguag cggaucuacc aucgguaagg cuuuugaagc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acugccuggg auuucggauc agugggcggu     1920

gcgcugaaca gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaauacga agaacgguuc gauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucca ccgccgucuc agcugacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3976
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 1 opt

<400>  3976
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagc ugcaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agaccuuaac uccugucggc     1680

aggcugauca cagccaaccc agugauuacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc ccccguucgg agacagcuac aucguuauag gcguggggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcugggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagauuuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uucgcaaauc cucauuggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauaucccuc augugucugg cauuaggggg agugcugauc     2100

uuccuaucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3977
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 1 opt

<400>  3977
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gaaaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggagguuacg      120

cgacgugguu ccgcuuacua uauguaccug gacagaaaug augccggcga agcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggacuu aggacacaug      240

ugugaugcca cuaugucaua cgagugcccu augcuggacg aaggcgugga gccagaugac      300

guagauuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggaa ucacgcgagu acaccaagca ccuuauucga      480

aucgaaaacu ggauauuuag aaaucccgga uucgcccugg cugccgcggc aaucgccugg      540

uugcucggca gcaguacauc ccagaaaguu auuuaucugg ugaugauccu gcucauugcu      600

ccugccuacu cuaucaggug cauaggcguc agcaaccggg acuuugugga ggggaugucc      660

ggagguacuu ggguagaugu uguguuagag cauggcgggu gugucaccgu gauggcacag      720

gacaagccaa caguggauau ugaacugguc accacgacug uuucaaauau ggcugaggug      780

cguaguuacu gcuaugaagc cagcaucucu gacauggcgu ccgauucacg cugucccaca      840

caaggagagg ccuaccuuga caaacagagc gacacccagu auguaugcaa gagaacuuug      900

guggauaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga aaaucucgag     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgacacg     1080

ggccaugaga ccgaugaaaa ucgagccaaa guggagauua caccaaacuc accccgcgcu     1140

gaagccacuc uugggggauu cggcucccug gguuuggacu gugagccuag aaccgggcuc     1200

gauuuuagug accuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagaa     1260

ugguuccacg auauaccacu cccguggcac gcaggcgccg acacuggaac accccauugg     1320

aauaacaagg aggcucuugu ggaguuuaaa gaugcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaagg ggccguccau accgcuuugg ccggagcacu agaggccgaa     1440

auggacggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggau     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg agacccucca uggcacuguc accguggaag uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagacaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gagaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggau acagccuggg acuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugauguc ggcugcucug uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3978
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 1 opt

<400>  3978
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaucg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auagugauug gcgucgggga gaaaaagauc     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaagg cuuuugaggc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac accgccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguag caucuccuug augugucucg cauuaggggg aguucugauu     2100

uuccugucaa ccgccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3979
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 1 opt

<400>  3979
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug ucaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug cugccuucac guuuacaaaa     1560

auaccugcag aaacccucca uggcacuguu accguggagg uacaguacgc ggguacagac     1620

gggccaugca agguccccgc ucaaauggcc guggauaugc agacccugac uccuguuggc     1680

agguuaauca cagcaaaccc agugauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgucauag gcguggggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaaag ccuuugaggc uaccguacgg     1860

ggcgccaagc gaauggcagu gcucggggac acagcuuggg auuucggaag uguuggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcggccuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuuc auguguuugg cucucggggg aguccugauc     2100

uuuuuaucaa cggcagugag ugccgacgug ggcugcucug ucgauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3980
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 1 opt

<400>  3980
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggcaa acuguccucu gggcaccuca agugccggcu gaaaauggac     1500

aagcugcgcc ugaaaggagu cagcuauuca uuguguacug ccgcauucac guuuacaaag     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agauuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa aaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaagaaaauu     1800

acucaccacu ggcauagguc uggaucaaca aucgguaagg cuuuugaggc caccgugcgu     1860

ggcgcaaaac ggauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa gggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca aaaacgguag cauauccuug augugucucg cacuuggggg aguuuuaauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3981
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 1 opt

<400>  3981
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gaggcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaagg ggccgugcau accgcuuugg ccggagcacu agaggccgaa     1440

auggacggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggau     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg agacccucca uggcacugug accguugaag ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgacaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gagaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggau acagccuggg acuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugaugug ggcugcucug uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3982
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 1 opt

<400>  3982
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc uggugccgac     1620

gggccaugca agguacccgc acaaauggcc gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugcuaaccc aguuauuaca gaaaguacgg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaaag ccuuugaggc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggaag ugugggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa ccgcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3983
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 1 opt

<400>  3983
gggagaaagc uuaccaugag aaucauuaac gccggcaagg agaaaaagag gcgcggggcu       60

gacaccaaug ugggaauagu cggucugcuc cuuacaacug caauggccgc ggaaguuacg      120

cggcgaggca gcgcuuacua uauguaccug gaucguaacg acgccgggga ggcaaucucc      180

uuccccacca cauugggaau gaauaaaugc uauauucaga ucauggauuu aggccacaug      240

ugugacgcca cuaugucuua cgaaugcccu augcuggaug agggugugga accagacgau      300

guagacuguu ggugcaacac cacaucaacc uggguggucu augggacuug ucaucacaag      360

aaaggagagg cuagaaggag ucggcgcgca gugacacuac cgagccauuc caccagaaag      420

cuccaaacga ggucucagac uuggcuggag ucgcgggaau acaccaagca ccuuauucgc      480

guugagaauu ggaucuuucg aaaccccggc uucgcccugg cugccgcggc aauagccugg      540

uugcucggcu caagcacaag ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu ccauuagaug caucggggug ucuaauaggg auuuuguaga aggaaugagc      660

gguggcacuu ggguugacgu ggucuuagag caugggggau gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug uguccaacau ggcugaagua      780

cggucauacu gcuaugaggc caguauuagc gauauggcgu cugacucccg uugucccaca      840

caaggcgaag ccuaccuuga caaacaguca gauacccagu augugugcaa gcgcacuuug      900

gucgacagag guugggggaa uggauguggc cuguucggca aagggagccu agugacaugc      960

gcaaaguuug cuugcaguaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uacaggauua ugcuguccgu ucacgguagc caacacucgg gcaugaucgu gaaugauacg     1080

gggcaugaga ccgacgagaa ccgggccaaa gucgaaauca caccaaauuc uccccgagcu     1140

gaggccacuc uuggaggcuu cgguucccug ggguuggauu gugaaccucg caccggccuc     1200

gacuuuucag aucuguauua cuuaacaaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggagccg auacuggcac accccauugg     1320

aacaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagagaca gaccguugug     1380

guccugggga gucaggaggg agccgugcau accgcuuugg ccggugcacu agaagccgag     1440

auggauggcg cuaaggggag gcugagcucc ggacaccuca aaugccggcu gaagauggac     1500

aaacuucgcc ugaagggcgu cucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca ugguacugug accguugagg ugcaguacgc ugggacagac     1620

ggcccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccuguggga     1680

agauuaauca cagccaaucc aguuauuacg gaaucaaccg agaacaguaa gaugaugcug     1740

gagcuagauc ccccguucgg cgacuccuac aucgugauag gggucggaga gaaaaagauu     1800

acucaccacu ggcauaggag cgguucuaca aucggcaagg cuuuugaagc caccgugcgu     1860

ggggcacggc gaauggcugu acucggagac acagccuggg auuucggcuc aguggguggg     1920

gcgcugaaua gucuuggcaa aggaauccac cagauuuucg gcgccgcuuu uaagagcuug     1980

uucgggggaa uguccugguu uucucaaauc cugauuggca cucuccugau guggcuuggu     2040

cugaacacca aaaauggguc gaucagcuug augugucucg cacugggagg cguuuuaaua     2100

uuccugucca cggccgucuc agcugacgug gguugcagug uggauuuuuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3984
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 1 opt

<400>  3984
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gaaaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggagguuacg      120

cgacgugguu ccgcuuacua uauguaccug gacagaaaag augccggcga agcaaucucu      180

uuccccacca cauuggggau gaauaagugc uauauucaga ucauggacuu aggacacaug      240

ugugaugcca cuaugucaua cgagugcccu augcuggacg aaggcgugga gccagaugac      300

guagauuguu ggugcaacac cacaaguacc uggguggucu augguacuug ucaucacaaa      360

aagggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggaa ucacgcgagu acaccaaaca ccuuauucga      480

guugaaaauu ggaucuuuag aaaccccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaggug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaucggg acuuuguaga ggggaugucc      660

ggagguacuu ggguugaugu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gacaaaccaa cagucgauau agaacugguu accacgacug ugucaaacau ggcugaggua      780

cguaguuacu gcuaugaagc cagcauuucu gacauggcgu ccgauucacg cugucccaca      840

caaggagagg ccuaccuuga caagcagagc gacacccagu augugugcaa aagaacuuug      900

gucgauaggg gcugggguaa ugggugugga cuguucggca agggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa aaagaugacc gggaaaucca uccagccuga aaaccucgag     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaaugacacg     1080

ggccaugaga ccgaugaaaa ccgagccaag gucgagauca caccaaauuc accccgcgcu     1140

gaagccacuc uugggggauu cggcucccug gguuuggacu gugagccuag aaccgggcuc     1200

gauuuuagug accuguauua cuuaacaaug aacaauaaac acuggcuggu gcauaaggaa     1260

ugguuccacg auauuccacu cccguggcac gcaggcgccg acacuggaac accccauugg     1320

aacaauaaag aggcucuugu agaguuuaag gaugcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaagg ggccgugcau accgcuuugg ccggagcacu agaggccgaa     1440

auggacggug cuaaaggccg gcuguccucu gggcaccuca agugccgccu gaaaauggau     1500

aagcugagac ugaaaggagu cagcuauuca uuguguacug ccgcauucac guuuacaaag     1560

auaccugcgg agacccucca uggcacugug accguugaag ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgacaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gagaguaccg agaacuccaa aaugaugcug     1740

gaacuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaagaaaauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggau acagccuggg acuucggaag ugugggcggu     1920

gcgcugaaua gccuugggaa gggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaacacca aaaaugguag cauauccuug augugucucg cacuuggggg aguuuuaauc     2100

uuccugucaa cggccgucag ugcugaugug ggcugcucug uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3985
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 1 opt

<400>  3985
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggugu ccgacucacg cugucccaca      840

caaggagaag cguaccuuga caaacagagc gauacccagu augucugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu aguuacaugc      960

gccaaguuug caugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu gcacggaucg caacacucug guaugaucgu caacgauacg     1080

ggccaugaga ccgacgagaa ucgagcuaaa guggaaauca caccaaacuc accccgcgcc     1140

gaggcuacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu acauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auacuggaac accccauugg     1320

aauaacaagg aagcccuugu ugaguuuaaa gacgcucacg cgaagaggca gaccgugguc     1380

gugcugggca gccaggaggg ggcaguccau accgccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug cugccuucac guuuacaaaa     1560

auaccugcag aaacccucca uggcacuguu accguggagg uacaguacgc ggguacagac     1620

gggccaugca agguccccgc ucaaauggcc guggauaugc agacccugac uccuguuggc     1680

agguuaauca cagcaaaccc agugauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgucauag gcguggggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaaag ccuuugaggc uaccguacgg     1860

ggcgccaagc gaauggcagu gcucggggac acagcuuggg auuucggaag uguuggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcggccuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cuuuaggggg aguccugauc     2100

uuccugucaa cggcagugag ugccgacgug ggcugcucug ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3986
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 1 opt

<400>  3986
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccaaug uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacguggua gcgcuuacua uauguaccug gauagaaacg acgccggcga ggcaaucucc      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucuua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacau gaccucaaca uggguggucu augguaccug ucaucacaag      360

aaaggggagg cuaggcggag ucgcagagca gugacucuac cgagccauuc cacaaggaag      420

cuccaaaccc ggucucagac guggcuggag ucgcgcgaau acacuaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggcu caagcaccag ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu ccauuaggug caucggcgug ucuaaucggg auuuuguaga agggaugagc      660

ggagguacau ggguugacgu ggucuuagag cauggcgggu gugugacugu gauggcacag      720

gauaagccaa ccgucgacau agagcugguu acaaccacgg uguccaacau ggcugaagua      780

cguucauacu gcuaugaggc caguauuagc gauauggcgu cugacucccg cugucccacu      840

caaggagaag ccuaccuuga caaacaguca gauacacagu augugugcaa gagaaccuug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcagccu agugacuugc      960

gcaaaguuug cuugcaguaa gaaaaugaca gggaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu ucacggaagc caacacucgg guaugaucgu gaaugauacc     1080

ggccaugaga cggacgagaa ccgagccaaa gucgaaauca ccccaaauuc uccccgcgcu     1140

gaggccacac uugggggauu cggcucccug gguuuggauu gugaaccuag aacugggcuc     1200

gacuuuucag aucuguauua cuuaaccaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacaggaac uccccauugg     1320

aacaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gucaggaggg ggccgugcau acagcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguaccg ccgcauucac uuuuacgaaa     1560

auaccugcgg aaacacucca uggcaccgug acuguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugccaaucc aguuauuaca gaaucaacgg agaacaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguag cggaucuacu aucgguaagg cuuuugaagc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac accgccuggg auuucggauc agugggcggu     1920

gcgcugaaua gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaacacua agaaugguuc gaucagcuug augugucucg cauuaggggg aguucugaua     2100

uuccugucca ccgccgucuc agcugacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3987
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 1 opt

<400>  3987
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccgcac uugggggauu cggcucccug gguuuggauu gugaaccuag aacugggcuc     1200

gacuuuagug aucuguauua cuuaaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcug auacaggaac uccccauugg     1320

aauaacaagg aagcgcuugu agaguuuaaa gacgcacacg ccaagaggca gaccguugug     1380

guccugggca gccaggaggg ggcugugcau acagccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguaccg cagcguucac uuuuacgaaa     1560

auaccugcug aaacacucca uggcaccgug acuguugagg ugcaguacgc cgguaccgac     1620

gggccaugca agguacccgc acaaauggcc gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugcuaaccc aguuauuaca gaaaguacgg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaaag ccuuugaggc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggaag ugugggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaaca cugaucggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauuuccuug augugucucg ccuuaggggg aguucugaua     2100

uuccugucaa cggcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3988
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 1 opt

<400>  3988
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gaaaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggagguuacg      120

cgacgugguu ccgcuuacua uauguaccug gacagaaaug augccggcga agcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggacuu aggacacaug      240

ugugaugcca cuaugucaua cgagugcccu augcuggacg aaggcgugga gccagaugac      300

guagauuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggaa ucacgcgagu acaccaagca ccuuauucga      480

guugaaaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

uuagccuacu cuauuaggug caucggcgug agcaaccggg acuuuguaga ggggaugucc      660

ggagguacuu ggguugaugu gguccuggag cauggcgggu gugugaccgu gauggcacag      720

gacaagccua cagucgauau agaacuuguu accacgacug ugucaaauau ggcugaggua      780

cguaguuacu gcuaugaagc cagcauuucu gacauggcgu ccgauucacg cuguccaaca      840

caaggagagg ccuacuugga caaacagagc gacacccagu augugugcaa gagaacucug      900

gucgauaggg gcugggguaa cgggugugga cuauucggca aaggcagucu cgugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagcccga aaaucuggag     1020

uaccggauua ugcuuagcgu ucacggaucg caacacucug guaugaucgu gaacgacacg     1080

ggccaugaga ccgaugaaaa ucgagccaaa gucgagauca caccuaacuc cccacgcgcu     1140

gaagccacuc ugggaggguu cggcucauug ggucucgacu gugagcccag aaccgggcug     1200

gauuuuagug acuuauauua ccugacaaug aauaacaagc acuggcucgu gcauaaagaa     1260

ugguuccacg auauuccucu uccauggcac gcaggcgccg acacuggaac cccgcauugg     1320

aauaacaagg aggcucuggu agaguuuaaa gaugcgcacg caaagaggca gacaguugug     1380

gucuugggca gccaggaagg ggccgugcau accgcucuag ccggagcacu ggaggccgaa     1440

auggacggug cuaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggau     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug ccgcauucac guuuacaaaa     1560

auacccgcgg agacccugca uggcacugug accguugaag ugcaguacgc ugguacagac     1620

gggccuugca agguaccagc ccaaauggca gucgacaugc agaccuuaac ucccgugggc     1680

aggcugauca cagccaaccc uguuauuacg gagaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc cacccuucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acugggggau acagccuggg acuucggaag ugugggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagauuuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uucgcaaauc cucauuggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauaucccuc augugucugg cauuaggggg aguucugauc     2100

uuccuaucaa cggccgucag ugcugaugug ggcugcucug uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3989
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 1 opt

<400>  3989
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcaua agcaaccggg auuuugugga agggaugucc      660

ggagguacuu ggguagacgu uguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcauacug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuuccg aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acaucccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gucaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auuccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauca cagccaaccc agugauaacg gaaucaaccg agaauaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac auuguuaucg gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguag cggaucuaca auugguaagg cuuuugaagc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggauc aguaggcggu     1920

gcgcugaaca gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaaua cugaucggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguuc gauuagcuug augugucucg cauuaggggg agugcugauc     2100

uuccugucca cggccguuuc agcugacguc ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3990
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 1 opt

<400>  3990
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacacu      240

ugugacgcca ccaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac aaccaguacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacccuac cgucccauuc uauuaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauccga      480

guugagaacu ggauauuuag aaaucccgga uucgcccugg cugccgcggc aaucgccugg      540

uugcucggca gcaguacauc ccagaaagug auuuaucugg ucaugauccu gcucauugcu      600

ccugccuacu cuaucaggug cauaggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau ugagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauua cagccaaccc aguuaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auagugauug gcgucgggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaaugcaa agaacgguag caucuccuug augugucucg ccuuaggggg aguucugauu     2100

uuccugucaa ccgcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3991
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 1 opt

<400>  3991
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cucaaaggag ccggcgcgca gugacacuac cgucccauuc uaccagaaag      420

cuccagacga ggucgcagac uuggcuggag ucacgggaau acaccaagca ccuuauucgc      480

guugagaacu ggaucuuucg aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuagaug caucggcgug agcaacaggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacaa      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cggaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg uugucccaca      840

cagggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gcgcacuuug      900

gucgacagag gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccaaccuga gaaucucgaa     1020

uacaggauua ugcugagcgu ucacggaucg cagcacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgggccaaa gucgaaauca caccaaacuc accccgagcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccucg caccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagagaca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggcag gcuguccucu gggcaccuca aaugccggcu gaagauggac     1500

aaacuucgcc ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaauacgc ugguacagac     1620

gggccaugca agguacccgc ccagauggca gucgauaugc agacccugac uccugugggc     1680

agauuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcauagguc uggaucaaca aucgguaagg cuuuugaggc caccgugcgu     1860

ggcgcaaaac ggauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac caaauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcagauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3992
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 1 opt

<400>  3992
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaagccc ggucgcagac guggcuggag ucacgcgaau acacuaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcucugg ccgcggcagc cauagcuugg      540

uugcucggca gcaguaccuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcauacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacau ggguugacgu ggucuuagag cauggcgggu gugugacugu gauggcucag      720

gauaagccaa ccgucgacau agagcugguu acaaccacgg ugucaaauau ggccgaagua      780

cguaguuacu gcuaugaggc gagcauuucu gauauggccu ccgacucacg cugucccacu      840

caaggagaag cauaccuuga caaacagagc gauacacagu augugugcaa gagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacuugc      960

gcuaaguuug ccugcucuaa gaaaaugaca gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cggacgagaa ucgagcuaaa gucgaaauca ccccaaacuc accccgcgcc     1140

gaggcaacac uugggggauu cggcucccug gguuuggauu gugaaccuag aacugggcuc     1200

gacuuuagug aucuguauua cuuaaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcug auacaggaac uccccauugg     1320

aauaacaagg aagcgcuugu agaguuuaaa gacgcacacg ccaagaggca gaccguugug     1380

guccugggca gccaggaggg ggcugugcau acagccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguaccg cagcguucac uuuuacgaaa     1560

auaccugcug aaacacucca uggcaccgug acuguugagg ugcaguacgc cgguaccgac     1620

gggccaugca agguacccgc acaaauggcc gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugcuaaccc aguuauuaca gaaaguacgg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaaag ccuuugaggc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggaag ugugggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa ccgcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3993
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 1 opt

<400>  3993
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaucug acgccggcga ggcaaucuca      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcgguc ccgcagagca gugacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgaau acaccaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca guuccacauc ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugaguaacau ggcugaagua      780

cguagcuacu gcuaugaggc cucuauuucc gauauggcgu cagacagccg cugucccaca      840

caaggagaag ccuaccuuga caaacagagu gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugacaugc      960

gcaaaguuug cuugcuccaa gaaaaugacc gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagccaaa gucgaaauca caccaaauuc accccgcgcu     1140

gaggccacuc uugggggauu cggcagucug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagcg aucuguauua cuuaacaaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aacaauaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaauccaccg agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc aggaaguaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaagc gaauggcugu acucggggac acagccuggg auuucggaag cgugggcggu     1920

gcgcuuaauu cccuggggaa aggcauccac cagauuuucg gagccgcuuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaacacca agaaugguuc cauaucauug augugucucg cauuaggggg aguucugauc     2100

uuccugagua cggccgucuc ugcugacgug ggcugcagcg uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3994
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 1 opt

<400>  3994
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagc cgaaguuacc      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa ccaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac aaccagcacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacuuc ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugaguaacau ggcagaagua      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccacc      840

caaggagaag cguaccuuga caaacagagu gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugaccugc      960

gccaaguuug caugcuccaa gaaaaugacg gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacc     1080

ggccaugaga cagacgagaa ccgagcuaaa gucgaaauca cuccaaauuc accccgcgcc     1140

gaggcuaccc uugggggauu cggcagucug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac accggcgccg auacaggaac uccccauugg     1320

aacaauaagg aagcacuugu agaguuuaaa gacgcccacg cuaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggcggugcau acggcauugg ccggagcucu agaagccgag     1440

auggauggug caaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacag ccgcuuucac cuuuacuaaa     1560

auaccugccg aaacccucca uggcacagug accguugagg ugcaguacgc agguacugac     1620

gggccaugca agguacccgc gcaaauggcu gucgauaugc agacacugac gccugugggc     1680

agguuaauca ccgccaaccc aguuauuacu gaaggaacag agaacuccaa gaugaugcug     1740

gagcuagauc ccccguucgg cgacagcuac aucgugauag gggucggaga aaagaagauu     1800

acccaccacu ggcaucguuc ugguucaaca aucggcaaag cauuugaggc cacugugcga     1860

ggggcuaagc ggauggccgu acucggagac accgcauggg auuucggcag uguggguggg     1920

guucugaaua gccuuggcaa aggaauccac cagauuuucg gcgcugccuu uaaguccuug     1980

uucgggggaa ugucuugguu uucgcaaauc cugauuggca cgcuccugau guggcuuggu     2040

cugaacacca agaaugggag cauauccuug augugucucg cgcugggagg cgucuuaauc     2100

uuccugucaa cagccgugag ugcugacgug gguugcucug ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3995
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 1 opt

<400>  3995
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgc aauggcucag      720

gauaagccaa caguggacau agagcugguc accacgacug uuucaaauau ggccgaagug      780

cguaguuacu gcuaugaggc gagcauuucu gauauggccu ccgacucacg cugucccaca      840

caaggagaag cauaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcuaaguuug ccugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu gcacggaucg caacacucug guaugcuugu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagcuaaa guggaaauca caccaaacuc accccgcgcc     1140

gaggcaacuc ugggaggguu cggcuccuug ggucucgauu gugaaccuag aaccgggcug     1200

gacuuuagug auuuauauua ccugacaaug aauaacaagc acuggcucgc ccauaaagag     1260

ugguuccacg acaucccacu uccguggcac gcuggcgcgg caacuggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gaugcucacg ccaagaggca gaccguggua     1380

guuuugggca gccaggaggg ggcagugcau accgcccuag cuggagcccu ggaagcagag     1440

auggacggug cgaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggau     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug cugccuucac guuuacaaaa     1560

auuccugcag aaaccgugga cggcacuguc accguggagg gucaguacgg gggcacagac     1620

ggaccaugca agguucccgc ccaaauggcu guggauaugc agacccugac uccuguaggc     1680

agguuaauaa cagccaaccc agucaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg ggacagcuac auugugaucg gaguugguga gaaaaagaua     1800

acucaucacu ggcaccguuc uggcucaaca auugggaaag cauuugaggc uaccgugcgg     1860

ggagccaagc gaauggcggu ccucggcgac acagccuggg auuucgguag uguagggggc     1920

gcucugaaca gccuuggaaa aggcauccau cagaucauug gggcagccuu caaguccuug     1980

uuuggaggca ugucuugguu cucgcaaauc cugauuggua cucuccugau guggcuuggg     2040

cugaauacca agaacggaag cauauccuug augugucucg cucugggcgg uguguuaauc     2100

uuucugucaa cggccgugag uggcgggguu ggaugcucug ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3996
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 1 opt

<400>  3996
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagc cgaaguuacc      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa ccaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac aaccagcacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacuuc ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugaguaacau ggcagaagua      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccacc      840

caaggagaag cguaccuuga caaacagagu gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugaccugc      960

gccaaguuug caugcuccaa gaaaaugacg gggaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacc     1080

ggccaugaga cagacgagaa ccgagcuaaa gucgaaauca cuccaaauuc accccgcgcc     1140

gaggcuaccc uugggggauu cggcagucug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auaccggaac accccauugg     1320

aacaauaagg aagcccuugu agaguuuaaa gacgcucacg cgaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggcagugcau acugccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacgg cugccuucac auuuuccaaa     1560

auaccugcag aaacccucca uggcacugug accgaggaag uucaguacgc ggguacagac     1620

gggccaugca aggugcccgc ucaaauggcc guagauaugc agacccugac uccugucggc     1680

agguuaauca cagcaaaccc agugauuacg gagagcaccg agaacucuaa gaugaugcug     1740

gaacuagauc ccccguucgg agacucauac aucguuauag gcguggggga gaaaaagauu     1800

acucaccacu ggcaucguag uggaagcaca aucgguaaag ccuuugaagc uaccguccgg     1860

ggcgccaagc gaauggcagu gcucggggac acagcuuggg auuucggauc cguaggcggu     1920

gcccugaaua gccuugggaa aggcauccac cagauuuucg gagcggccuu uaagucguug     1980

uucggcggga ugagcugguu uucccaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaacacca agaaugguuc aauaaguuug augugucucg cuuuaggggg agugcugauc     2100

uuccugucua cggcaguuag cgccgacguc ggcugcuccg uggauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3997
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 1 opt

<400>  3997
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagc cgaaguuacc      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa ccaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac aaccagcacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacuuc ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugaguaacau ggcagaagua      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccacc      840

caaggagaag cguaccuuga caaacagagu gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugaccugc      960

gccaaguuug caugcuccaa gaaaaugacg gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacc     1080

ggccaugaga cagacgagaa ccgagcuaaa gucgaaauca cuccaaauuc accccgcgcc     1140

gaggcuaccc uugggggauu cggcagucug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auaccggaac accccauugg     1320

aacaauaagg aagcccuugu agaguuuaaa gacgcucacg cgaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggcagugcau acugccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacgg cugccuucac auuuaccaaa     1560

auaccugcag aaacucucca uggcaccgug acaguugagg ugcaguacgc ggguaccgac     1620

gggccaugca agguacccgc ucaaauggcc gucgauaugc agacucugac accugugggc     1680

agguuaauca cggcaaaccc aguuauuacc gaauccacug agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcgucgggga aaagaagauu     1800

acacaccacu ggcaucguuc aggaaguacc aucgguaaag ccuuugaggc uacagugcgg     1860

ggcgccaagc gaauggcagu acucggggac acugcuuggg auuucggaag cgugggcggu     1920

gcccuuaauu cccuggggaa aggcauccac cagauuuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaacacga agaaugguuc cauaucauug augugucucg cuuuaggggg aguucugauc     2100

uuccugagua ccgcagucuc ugccgacgug ggcugcagcg uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3998
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 1 opt

<400>  3998
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagu ugaagugacc      120

cgacguggua augccuacua uauguaccug gauagauccg acgcgggcga ggcuaucucu      180

uuccccacaa cuauggggau gaacaaaugc uauauucaga ucauggauuu gggacacaug      240

ugugacgcca ccaugucaua cgaaugcccu auguuagaug agggcguaga accagacgau      300

guggacuguu ggugcaauac aaccaguacu ugggucgugu augguacaug ucaucacaag      360

aaaggggagg caaggcggag ccgcagagcc guuacccugc cgucccauuc uacgaggaag      420

cuacaaacuc ggucgcagac cuggcucgag ucacgcgaau acacaaagca ccugauucga      480

guggagaacu ggaucuuuag aaaucccgga uucgcucuug cagccgcugc cauagcgugg      540

cuguugggca gcaguacuuc ccagaaaguc aucuaucucg ugaugauucu gcugaucgca      600

cccgccuacu cuauuaggug caucggcgua agcaaccggg auuuuguuga agggaugucc      660

ggagguaccu ggguggacgu cgugcucgag cauggcgggu gugugacagu cauggcucag      720

gauaagccaa ccguugacau agaguuagug acgacuacag uaucaaauau ggccgaagug      780

cguaguuacu gcuaugaggc aagcauuucu gauauggcuu ccgacucacg cugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacucagu augucugcaa gagaacacuu      900

guggacaggg gcugggguaa cgggugugga uuguucggca aaggcagucu gguuaccugc      960

gcgaaguuug ccugcucuaa gaaaaugacg gggaagucca uccagccuga gaaucuagaa     1020

uaccggauua ugcucagcgu gcacggaucg caacacucug guaugaucgu caacgauacc     1080

ggccaugaga cagacgagaa ucgagcaaaa guggaaauca cuccaaacuc cccucgcgcu     1140

gaggccaccc uugggggauu cggcucacug ggucuggauu gugaaccuag aacaggguug     1200

gacuuuagug aucucuauua ccugacuaug aauaacaagc acugguuagu acauaaagag     1260

ugguuccacg acauuccacu gccguggcac gcuggcgccg auaccggaac accccauugg     1320

aauaacaagg aagcacucgu ugaguuuaaa gacgcccacg cuaagaggca gaccgugguc     1380

gugcuuggca gccaggaggg ggcgguccau acugcacugg ccggagcuuu ggaagccgag     1440

auggauggug caaagggccg gcuauccucu gggcaccuga aaugccgccu caagauggac     1500

aaacugagac uuaagggagu gagcuauuca cuguguacgg ccgcuuucac auuuaccaaa     1560

auaccugccg aaacuuugca uggcaccguu acaguggagg uacaguacgc agguaccgac     1620

gggccaugca agguccccgc gcaaauggcu guggauaugc agacucucac accuguuggc     1680

aggcugauca cggccaaccc agugauuacc gaaaguacug aaaauuccaa gaugauguua     1740

gagcuggauc ccccguucgg agacagcuac aucgucauag gcguggggga gaaaaagauu     1800

acacaccacu ggcaucguuc uggaucaacc aucgguaaag cauuugaggc cacaguacgg     1860

ggcgcuaagc gaauggccgu gcuaggggac acugcauggg auuucggaag uguuggcggu     1920

gcucucaaca gccuggggaa aggcauccac cagauuuucg gagccgcguu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauc uugauuggaa cccugcucgu cuggcugggc     2040

cuuaauacga agaacgguag cauaucccug auguguuugg cccucggggg agugcugauc     2100

uucuuaucaa ccgcugugag ugcagacguc ggcugcucug uugauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  3999
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 1 opt

<400>  3999
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagc cgaaguuacc      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa ccaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac aaccagcacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacuuc ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugaguaacau ggcagaagua      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccacc      840

caaggagaag cguaccuuga caaacagagu gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugaccugc      960

gccaaguuug caugcuccaa gaaaaugacg gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacc     1080

ggccaugaga cagacgagaa ccgagcuaaa gucgaaauca cuccaaauuc accccgcgcc     1140

gaggcuaccc uugggggauu cggcagucug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auaccggaac accccauugg     1320

aacaauaagg aagcccuugu agaguuuaaa gacgcucacg cgaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggcagugcau acugccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacgg cugccuucac auuuaccaaa     1560

auaccugcag aaacucucca uggcaccgug acaguugagg ugcaguacgc ggguaccgac     1620

gggccaugca agguacccgc ucaaauggcc gucgauaugc agacucugac accugugggc     1680

agguuaauca cggcaaaccc aguuauuacc gaauccacug agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcgucgggga aaagaagauu     1800

acacaccacu ggcaucguuc aggaaguacc aucgguaaag ccuuugaggc uacagugcgg     1860

ggcgccaagc gaauggcagu acucggggac acugcuuggg auuucggaag cgugggcggu     1920

gcccuuaauu cccuggggaa aggcauccac cagauuuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaacacga agaaugguuc cauaucauug guuugucucg cuuuaggggg aguccugauc     2100

uuccugagua ccgcaguguc ugccgacgug ggcugcagcg ucgauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4000
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 1 opt

<400>  4000
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagu ugaagugacc      120

cgacgugguu ccgccuacua uauguaccug gauagaucug acgcgggcga ggcuaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca ccaugaguua cgaaugcccu augcuggaug agggcguaga accagacgau      300

guggacuguu ggugcaacac aaccagcacu ugggucgugu augguacaug ucaucacaag      360

aaaggggagg caaggcgguc ccgcagagcc guuacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guggagaauu ggaucuuuag aaaccccgga uucgcucugg cagccgcugc cauagcgugg      540

uugcucggca guuccacuuc ucagaaaguc aucuaucugg ugaugauucu gcucaucgcc      600

ccugcauaca gcauuaggug caucggcgua uccaaucggg auuuuguuga agggauguca      660

ggagguaccu ggguggacgu cguguuagag cauggcgggu gugugacagu cauggcucag      720

gauaagccag ccguugacau agagcuggug accacgacug uaaguaacau ggcagaagug      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccaca      840

caaggagaag cguaccuuga caaacagagu gauacccagu augucugcaa gagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu aguuacaugc      960

gccaaguuug caugcuccaa gaaaaugacc gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu gcacggaucu caacacuccg guaugaucgu caaugauacg     1080

ggccaugaga ccgacgagaa ccgagcuaaa guggaaauca caccaaauuc accccgcgcc     1140

gaggcuacuc uugggggauu cggcagucug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagcg aucuguauua cuuaacaaug aacaauaagc acuggcuggu acauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auacuggaac accccauugg     1320

aacaauaagg aagcccuugu ugaguuuaaa gacgcucacg cgaagaggca gaccgugguc     1380

gugcugggcu cccaggaggg ggcaguccau accgccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gucauauagu uuguguacug cugccuucac guuuacaaaa     1560

auaccugcag aaacccucca uggcacuguu accguggagg uacaguacgc ggguacagac     1620

gggccaugca agguccccgc ucaaauggcc guggauaugc agacccugac uccuguuggc     1680

agguuaauca cagcaaaccc agugauuacg gaauccaccg agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgucauag gcguggggga aaagaagauu     1800

acucaccacu ggcaucguuc aggaaguaca aucgguaaag ccuuugaggc uaccguacgg     1860

ggcgccaagc gaauggcagu gcucggggac acagcuuggg auuucggaag cguuggcggu     1920

gcccuuaauu cccuggggaa aggcauccac cagauuuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cucuccuggu cuggcuuggc     2040

cugaacacca agaaugguuc cauaucauug acgugucucg cuuuaggggg agugcugauc     2100

uuccugagua ccgcaguguc ugccgacguc ggcugcagcg uugauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4001
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 1 opt

<400>  4001
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgu ugaagugacg      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa cuaugaguua cgaaugcccu augcuggaug agggcguaga accagacgau      300

guggacuguu ggugcaacac cacaagcacc ugggucgugu augguacuug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu guuacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgaau acaccaagca ccuuauucga      480

guggagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgu cauagcgugg      540

uugcucggca guuccacauc ucagaaagug aucuaucugg uaaugauucu gcucaucgcc      600

ccugcauaca gcauuaggug caucggcguu uccaaucggg auuuugugga agggauguca      660

ggagguacuu gggucgacgu gguguuagag cauggcgggu gugucaccgu uauggcucag      720

gauaagccaa caguggacau agagcuggua accacgacug ugaguaacau ggccgaaguc      780

cguagcuacu gcuaugaggc aucuauuucc gauauggcuu cagacagccg cugucccaca      840

caaggagaag ccuaccuuga caaacagagu gauacccagu augugugcaa gagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugacaugc      960

gcgaaguuug ccugcuccaa gaaaaugacc gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ccacggaucu caacacuccg guaugaucgu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagcaaaa guagaaauca caccaaauuc accccgcgcu     1140

gaggccacuc uugggggauu cggcagucug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagcg aucuguauua cuuaacaaug aacaauaagc acuggcuggu ucauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcuggcgccg auacuggaac accccauugg     1320

aacaauaagg aagcacuugu ggaguuuaaa gacgcccacg cuaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcggugcau accgcauugg ccggagcucu agaagccgag     1440

auggauggug caaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu uucauauagu uuguguacug ccgcuuucac guuuacaaaa     1560

auaccugccg aaacccucca uggcacugug accguagagg uccaguacgc agguacagac     1620

gggccaugca aggugcccgc gcaaauggcu guugauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc agucauuacg gaauccaccg agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcguagggga aaagaagauu     1800

acucaccacu ggcaucguuc aggaaguaca aucgguaaag cauuugaggc caccgugcgg     1860

ggcgcuaagc gaauggccgu ucucggggac acagcauggg auuucggaag cgucggcggu     1920

gcucuuaauu cccuggggaa aggcauccac cagauuuucg gagccgcguu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cucuccuggu guggcuuggc     2040

cugaacacca agaaugguuc cauaucauug acgugucucg ccuuaggggg agugcugauc     2100

uuccugagua ccgcugucuc ugcagacguu ggcugcagcg uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4002
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 1 opt

<400>  4002
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgu ugaagugacg      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacca cauugggggu aaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa cuaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

gucgacuguu ggugcaacac cacaagcacc ugggugguuu augguacuug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgaau acaccaagca ccuuauucga      480

gucgagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacauc ucagaaagug aucuaucugg uaaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcguu uccaaucggg auuuugugga agggauguca      660

ggagguacuu gggucgacgu gguguuagag cauggcgggu gugucaccgu uauggcccag      720

gauaagccaa caguggacau agagcuggua accacgacug ugaguaacau ggcagaaguc      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccaca      840

caaggagaag cguaccuuga caaacagagu gauacccagu augugugcaa gagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugacaugc      960

gccaaguuug caugcuccaa gaaaaugacc gggaagucca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ccacggaucu caacacuccg guaugaucgu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagcuaaa guagaaauca caccaaauuc accccgcgcc     1140

gaggcuacuc uugggggauu cggcagucug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagcg aucuguauua cuuaacaaug aacaauaagc acuggcuggu ucauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auacuggaac accccauugg     1320

aacaauaagg aagcccuugu ggaguuuaaa gacgcucacg cgaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcagugcau accgccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu uucauauagu uuguguacug cugccuucac guuuacaaaa     1560

auaccugcag aaacccucca uggcacugug accguagagg uccaguacgc ggguacagac     1620

gggccaugca aggugcccgc ucaaauggcc guugauaugc agacccugac uccugugggc     1680

agguuaauca cagcaaaccc agucauuacg gaauccaccg agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcguagggga caaaaagauu     1800

acucaccacu gguaucguuc aggaaguaca aucgguaaag ccuuugaagc uaccgugcgg     1860

ggcgccaagc gaauggcagu ucucggggau acagcuuggg acuucggaag cgucggcggu     1920

gcccugaaua gccuugggaa aggcauccau cagauuuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cucuccuggu guggcuuggc     2040

cugaacacca agaaugguuc cauaucauug acgugucucg cuuuaggggg agugcugauc     2100

uuccugagua ccgcagucuc ugccgauguu ggcugcagcg uggacuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4003
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 1 opt

<400>  4003
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgu ugaagugacg      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcaa agccaucuca      180

uuccccacca cauugggggu aaauaagugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa cuaugaguua cgagugcccu augcuggaug aaggcgugga gccagacgau      300

gucgacuguu ggugcaacac cacaagcacc ugggugguuu augguacuug ucaucacaaa      360

aagggggaag ccaggcgguc ccgcagagcu gugacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgagu acaccaaaca ccuuauucga      480

gucgaaaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacauc ucagaaggug aucuaucugg uaaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguacuu gggucgacgu gguguuagaa cauggcgggu gugucaccgu uauggcccag      720

gauaaaccaa caguggacau agagcuggua accacgacug ugaguaacau ggcagagguc      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccaca      840

caaggagaag cguaccuuga caagcagagu gauacccagu augugugcaa aagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugacaugc      960

gccaaguuug caugcuccaa aaagaugacc gggaaaagca uccagccuga aaaccucgag     1020

uaccggauua ugcugucggu ccacggaucu caacacuccg guaugaucgu gaaugauauc     1080

ggccaugaaa cggacgagaa ccgagcuaag guagagauua ccccaaauuc accccgcgcc     1140

gaagcuacac uugggggauu cggcagucug gguuuggauu gugagccuag aacugggcuc     1200

gacuuuagcg aucuguauua cuuaaccaug aacaauaaac acuggcuggu ucauaaggaa     1260

ugguuccacg acauaccacu cccguggcac gccggcgcag auacaggaac uccccauugg     1320

aacaauaaag aggcccuugu ggaauuuaag gacgcucacg cgaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcagugcau acagccuugg cuggagcccu agaggcagaa     1440

auggauggug ccaaaggccg gcugucuagc gggcaccuca agugccgccu gaaaauggac     1500

aagcugagac ugaaaggagu uucauauagu uuguguaccg cugccuucac uuuuacgaag     1560

aucccugcag agacacucca uggcaccgug acuguagaag uccaguacgc ggguaccgac     1620

gggccaugca aggugcccgc ucaaauggcc guugauaugc agacacugac cccugugggc     1680

agguuaauua cugcaaaccc agucaucaca gaguccacgg aaaacagcaa aaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac auagugauug gcguagggga caagaaaauc     1800

acccaccacu ggcaucguuc aggaaguacu aucgguaagg ccuuugaggc uacagugcgg     1860

ggcgccaaac gaauggcagu ucucggggau accgcuuggg acuucggaag cgucggcggu     1920

gcccuuaauu cccuugggaa gggcauucac cagaucuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauu cugauaggaa cacuccuggu guggcuuggc     2040

cugaacacua aaaaugguuc caucucauug accugucucg cuuuaggggg agugcugauu     2100

uuccugagua cggcagucuc ugccgauguu ggcugcagcg uggacuuuuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4004
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 1 opt

<400>  4004
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucacacugg uggcugucgc caucgcgugg      540

uugcucggca guuccacuuc ucagaaggug auuuaccugg uaaugauccu gcucauugca      600

cccgccuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguaccu gggucgacgu gguguuagaa cacggcgggu gugucacagu uauggcucag      720

gauaaaccua ccguggacau ugagcuggua acgacuacag ugaguaacau ggccgagguc      780

cguagcuacu gcuacgaagc aucuaucucc gauauggcuu cagacagccg cuguccaacc      840

caaggagagg ccuaucuuga caagcagagu gauacucagu acgugugcaa aagaacauug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugaccugc      960

gcgaaguuua cgugcuccaa aaagaugacc gggaaaagca uucagcccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu caacauuccg guaugaucgu gaaugauaca     1080

ggccacgaaa cugacgagaa ccgagccaag guagagguua ccccuaauuc accacgcgca     1140

gaagcuacac uugggggauu cggcagucug gguuuggauu gugagcccag aacugggcuc     1200

gacuuuagcg aucuguacua uuuaaccaug aacaauaaac auuggcuggu gcacaaggaa     1260

ugguuccacg acauuccucu cccauggcau gccggcgcug auacaggaac cccgcacugg     1320

aacaauaaag aggcccuugu cgaauuuaag gacgcacaug ccaagaggca gacugugguc     1380

gugcugggcu cccaggaggg ggcuguucac acggcguugg caggagcccu agaggcugaa     1440

auggauggug ccaaaggccg gcuguucucu gggcaucuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacuca uuguguacag cagccuuuac cuucacuaag     1560

guacccgcug agacccucca cggcacaguc accguggaag uucaguaugc cgguacugac     1620

gggccuugca aggugccagc acaaauggcg gucgauaugc agacacugac gcccgugggc     1680

agguuaauaa ccgcuaaccc uguaaucacu gagaguacag aaaacuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auugugaucg gcguugggga caagaaaaua     1800

acccaccauu ggcaccguuc uggaucaaca auugguaagg ccuucgaggc aacuguccgg     1860

ggcgccaaac gaauggcugu gcucggggau accgccuggg acuucggaag ugugggcggu     1920

gucuuuaaua gccuggggaa gggcauccau cagaucuucg gagcagcuuu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugaucggaa cgcugcucgu uuggcugggc     2040

cuuaacacca aaaaugguag cauuucccug acauguuugg cccucggggg agugaugaua     2100

uuccugucaa cugcggugag ugccgaugua ggcugcucug ucgacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4005
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 1 opt

<400>  4005
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca auuugggggu gaacaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua caaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaauac uaccagcaca uggguagugu acgguaccug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacucuac caucucacuc gacacggaag      420

cuccaaaccc gcucacagac guggcuggag agccgagagu auacuaagca ucuuauaaaa      480

guggaaaacu ggaucuuuag aaauccggga uucgcucugg ccgcgguugc aauugccugg      540

uugcucggca guuccaccuc ucagaaggug aucuaccugg ucaugauucu gcucaucgcu      600

cccgccuaua gcauaaggug cauuggcgug uccaaccggg auuuuguaga ggggauguca      660

ggagguacau ggguugacgu ggucuuagaa cacggcgggu gugugacugu gauggcacag      720

gauaaaccua ccgucgacau cgagcugguu acaaccacgg ugaguaauau ggcugaggua      780

cguagcuacu gcuacgaagc cucuauuucc gauauggcgu cagacagccg cuguccaacu      840

caaggagagg ccuaucuuga caagcagagu gauacacagu acgugugcaa aagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca agggcucucu agugacuugc      960

gcaaaguuua caugcuccaa aaagaugacc gggaaaagca uccagcccga aaaucucgag     1020

uaucggauca ugcugucggu ucacggaucu caacauuccg guaugauugu gaacgauaua     1080

ggccacgaaa cggacgagaa ucgagcuaag gucgagguga ccccuaacuc accacgcgcc     1140

gaagcuacac uugggggauu cggcagucug gguuuggauu gugagcccag aacugggcuc     1200

gacuuuagcg aucuguacua uuuaaccaug aauaacaaac auuggcuggu acacaaggaa     1260

ugguuccacg acaucccucu cccauggcau gccggcgcag auacaggaac uccgcacugg     1320

aauaacaaag aggcccuugu ugaauuuaag gacgcucaug cgaagaggca gaccgugguc     1380

gugcugggcu cccaggaggg ggcaguccac acagccuugg cuggagcccu agaggcagaa     1440

auggauggug ccaaaggccg gcuguucucu gggcaucuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacuca uuguguaccg cugccuuuac uuucacgaag     1560

guucccgcag agacacucca cggcaccgug acuguagaag uccaguaugc ggguaccgac     1620

gggccuugca aggugccagc ucaaauggcc guugauaugc agacacugac ccccgugggc     1680

agguuaauua cugcaaaccc ugucaucaca gagaguacgg aaaauuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auagugauug gcguagggga caagaaaauc     1800

acccaccauu ggcaccguuc uggaucaacu aucgguaagg ccuucgaggc uacagugcgg     1860

ggcgccaaac gaauggcagu ucucggggau accgcuuggg acuucggaag ugucggcggu     1920

guguuuaaca gccuggggaa gggcgugcau cagauuuucg gagccgcguu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauc uugauuggaa cacugcucgu cuggcugggc     2040

cuuaauacua aaaacgguag cauaucccug accuguuugg cccucggggg aguuaugauc     2100

uuccugucaa cggcugugag ugcagaugug ggcugcucug uagacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4006
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 1 opt

<400>  4006
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugu caaugauaua     1080

ggccacgaaa ccgacgagaa ccgagcaaag guggagguaa caccuaauuc accacgcgcu     1140

gaagccacuc uugggggauu cggcagucug gguuuggauu gugagcccag aaccgggcuc     1200

gacuuuagcg aucuguacua uuuaacaaug aacaauaaac auuggcuggu ucacaaggaa     1260

ugguuccacg acaucccucu cccauggcau gcuggcgccg auacuggaac cccgcacugg     1320

aacaauaaag aggcacuugu ggaauuuaag gacgcccaug cuaagaggca gacagucgug     1380

guccugggcu cccaggaggg ggcggugcac accgcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggccg gcuguucucu gggcaucuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu uagcuacuca uuguguacug ccgcuuuuac guucacaaag     1560

gugcccgccg agacccucca cggcacugua accgucgaag ugcaguaugc agguacagac     1620

gggccuugca agguuccagc gcaaauggcu guggauaugc agacccugac ucccgucggc     1680

agguuaauua cagccaaccc ugugaucacg gagaguaccg aaaacuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auaguaauug gcguggggga caagaaaauc     1800

acucaccauu ggcaccguuc uggaucaaca aucgguaagg cauucgaggc caccguucgg     1860

ggcgcuaaac gaauggccgu ccucggggau acagcauggg acuucggaag ugugggcggu     1920

guguuuaaua gccuggggaa gggcauucau cagaucuucg gagcugccuu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugauaggaa cucugcucgu cuggcugggc     2040

cuuaacacca aaaaugguag caucucccug acguguuugg cgcucggggg aguuaugauu     2100

uuccugucaa ccgccgugag ugcugaugug ggcugcucug uagacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4007
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 1 opt

<400>  4007
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca auuugggggu gaacaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua caaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaauac uaccagcaca uggguagugu acgguaccug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacucuac caucucacuc gacacggaag      420

cuccaaaccc gcucacagac guggcuggag agccgagagu auacuaagca ucuuauaaaa      480

guggaaaacu ggaucuuuag aaauccggga uucgcucugg ccgcgguugc aauugccugg      540

uugcucggca guuccaccuc ucagaaggug aucuaccugg ucaugauucu gcucaucgcu      600

cccgccuaua gcauaaggug cauuggcgug uccaaccggg auuuuguaga ggggauguca      660

ggagguacau ggguugacgu ggucuuagaa cacggcgggu gugugacugu gauggcacag      720

gauaaaccua ccgucgacau cgagcugguu acaaccacgg ugaguaauau ggcugaggua      780

cguagcuacu gcuacgaagc cucuauuucc gauauggcgu cagacagccg cuguccaacu      840

caaggagagg ccuaucuuga caagcagagu gauacacagu acgugugcaa aagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca agggcucucu agugacuugc      960

gcaaaguuua caugcuccaa aaagaugacc gggaaaagca uccagcccga aaaucucgag     1020

uaucggauca ugcugucggu ucacggaucu caacauuccg guaugauugu gaacgaugaa     1080

aaucgagcua aggucgaggu gacgccuaac ucaccacgcg ccgaggcuac ccuuggcggg     1140

uucggaaguc ugggcuugga cugugaaccc agaacagguc ucgauuuuag cgaccuguac     1200

uauuuaacua ugaauaacaa acacuggcug guacauaagg agugguucca cgauauaccu     1260

cucccauggc acgccggggc agacaccggc acaccgcauu ggaauaacaa agaagcccuu     1320

guugaguuua aggaugcuca cgcgaagagg cagacugugg ucgugcuggg aucccaggaa     1380

ggcgcagucc auaccgccuu ggcuggggcc cuagaggcag agauggacgg agccaaaggu     1440

cggcuguucu cuggccaccu caagugccgc cugaaaaugg auaaacuuag acugaaaggg     1500

gugagcuacu cauuguguac agcugccuuu accuucacua agguucccgc agaaacgcuc     1560

cauggaacag ugaccguaga gguccaguau gcgggcacug acgguccuug caaggugcca     1620

gcucaaaugg ccguugacau gcagacccug acacccgugg ggagguuaau caccgcaaac     1680

ccugucauua cugaaaguac agaaaauucc aaaaugaugc uggagcuaga uccacccuuu     1740

ggcgacagcu acaucgugau aggaguaggc gauaagaaaa uuacgcacca cuggcaucgu     1800

ucugggucaa ccaucggaaa ggccuucgag gcuacugugc ggggugccaa acgaauggca     1860

guucucggcg acacagcuug ggauuucggg agugucggag gcguguuuaa cagccugggu     1920

aaggggaucc accagauuuu cggcgccgcg uuuaaguccc uuuucggagg caugucuugg     1980

uuuucgcaaa ucuugauugg gacccugcuc guguggcugg gacuuaauac aaagaacggc     2040

agcauauccc ugacuuguuu ggcccucggu ggggucauga ucuuccuguc aaccgcuguu     2100

agugcagacg ugggaugcuc uguggauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4008
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 1 opt

<400>  4008
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucgcucugg uggccgucgc gaucgcaugg      540

uugcucggca guuccacauc ucagaaggug auuuaccugg uaaugauccu gcucauugcc      600

cccgcuuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguacuu gggucgacgu gguguuagaa cacggcgggu gugucaccgu uauggcccag      720

gauaaaccua caguggacau ugagcuggua accacgacug ugaguaacau ggcagagguc      780

cguagcuacu gcuacgaggc uucuaucucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgugugcaa aagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uucagcccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu caacauuccg guaugaucgu gaaugauacc     1080

ggccacgaaa cagacgagaa ccgagcaaag guagagguua cuccuaauuc accacgcgcu     1140

gaagccaccc uugggggauu cggcagucug gguuuggauu gugagcccag aacagggcuc     1200

gacuuuagcg aucuguacua uuuaacuaug aacaauaaac auuggcuggu gcacaaggaa     1260

ugguuccacg acauuccucu cccauggcau gcuggcgccg auaccggaac accgcacugg     1320

aacaauaaag aggcacuugu cgaauuuaag gacgcccaug cuaagaggca gaccgugguc     1380

gugcugggcu cccaggaggg ggcgguucac acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggccg gcuguucucu gggcaucuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacuca uuguguacgg ccgcuuuuac auucaccaag     1560

guacccgccg agacucucca cggcaccguc acaguggaag uucaguaugc agguaccgac     1620

gggccuugca aggugccagc gcaaauggcu gucgauaugc agacucugac acccgugggc     1680

agguuaauaa cggccaaccc uguaaucacc gagaguacug aaaacuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auugugaucg gcguugggga caagaaaaua     1800

acacaccauu ggcaccguuc uggaucaacc auugguaagg cauucgaggc cacaguccgg     1860

ggcgcuaaac gaauggccgu gcucggggau acugcauggg acuucggaag ugugggcggu     1920

gucuuuaaua gccuggggaa gggcauccau cagaucuucg gagcugccuu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugaucggaa cccugcucgu uuggcugggc     2040

cuuaacacga aaaaugguag cauuucccug accuguuugg cgcucggggg agugaugaua     2100

uuccugucaa cagccgugag ugcugaugua ggcugcucug ucgacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4009
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 1 opt

<400>  4009
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaacacaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucauuc gacccggaag      420

cuccagacgc gcucacagac uuggcuggag agccgagagu auaccaagca ccuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucgcucugg uggccgucgc gaucgcaugg      540

uugcucggca guuccacauc ucagaaggug auuuaccugg uaaugauccu gcucauugcc      600

cccgcuuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguacuu gggucgacgu gguguuagaa cauggcgggu gugucaccgu uauggcccaa      720

gauaaaccua caguggacau ugagcuggua accacgacug ugaguaacau ggcagagguc      780

cguagcuacu gcuacgaggc uucuaucucc gauauggccu cagacagccg cuguccaaca      840

cagggagaag cguaucuuga caagcagagu gauacccagu acgugugcaa aagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uucaacccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu cagcacuccg guaugaucgu gaaugauauu     1080

ggccaugaaa ccgacgagaa ccgagcaaag guagagguua caccuaauuc accacgcgcu     1140

gaagccacuc uugggggauu cggcagucug gguuuggauu gugagcccag aaccgggcuc     1200

gacuuuagcg aucuguacua uuuaacaaug aacaauaaac acuggcuggu gcauaaggaa     1260

ugguuccacg acauaccucu cccauggcac gcuggcgccg auacuggaac cccgcauugg     1320

aacaauaaag aggcacuugu cgaauuuaag gacgcccacg cuaagaggca gacagugguc     1380

gugcugggcu cccaggaggg ggcgguucau accgcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggccg gcuguucucu gggcaccuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacuca uuguguacug ccguaugcac ggcugccaag     1560

guccccgcag agacacucca uggcaccgug acuguugaag ugcaauaugc ggguaccgac     1620

gggccuugua aggucccagc ucagauggcc guggauaugc agacacugac ccccguaggc     1680

agguuaauca cugcaaaccc ugugauuaca gagaguacgg aaaacuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac aucguuauag gcgucgggga caagaaaauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaagg ccuucgaggc uacagugcgg     1860

ggcgccaaac gaauggcagu gcucggggau accgcuuggg acuuuggaag ugucggcggu     1920

guuuucaaua gccuggggaa gggcauccac caaauuuucg gagccgcguu uaagucccuu     1980

uucggcggga ugucuugguu uucgcagauc uugauuggaa cacugcucgu guggcugggc     2040

cuuaacacua aaaaugguag cauaucccug accugcuugg cccucggggg agugaugauc     2100

uuccugucaa cggcuguaag ugcagauguc ggcuguucug uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4010
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 1 opt

<400>  4010
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacgcaagca uagggaucgu gucccugcuc cuuaccacag ucauggccgc ggaaauuacu      120

cguagaggau cugcuuacua uauguaccug gauaggucag acgccgguaa ggcaaucagu      180

uucguuacga ccuugggcgu gaauaaaugc cacguacaga uuauggauuu agggcauaug      240

ugugacgcca caaugagcua ugagugcccc augcuggaug aaggagugga gccugacgau      300

gucgacuguu ggugcaacac uaccuccaca ugggugguuu acggcaccug ucaccauaag      360

aaaggugaag cucggcgcuc uagaagggca gugacucuac caucgcacuc aacacggaag      420

cuccaaaccc gcagccagac guggcuggag agucgagagu auacuaagca ucuuaucaaa      480

gucgaaaauu ggauauuuag aaacccgggg uucgcccugg uggcuguagc caucgcgugg      540

uugcucggau ccucuaccag ccagaagguu auuuaccugg ugaugauccu gcucauugca      600

cccgccuauu ccaucaggug cauaggcguc ucaaaucggg auuuugugga gggcaugagu      660

gggggaacau ggguggacgu cguuuuagaa cacgguggcu gugugacugu aauggcucag      720

gauaaaccua ccguggacau ugagcugguc acaaccacgg ugagcaacau ggccgagguu      780

cguucuuacu gcuacgaagc auccaucuca gauauggcua gcgacagucg cuguccaacu      840

caaggggagg ccuaucuuga caagcagucu gauacacagu acgugugcaa aagaaccuug      900

gucgacaggg gauggggcaa ugguuguggg cuguucggaa agggcucccu agugacuugc      960

gcgaaguuua caugcagcaa aaagaugacc ggcaaaucga uucagcccga aaaccucgag     1020

uaucggauca ugcugucugu acacgggucc caacauucag gaaugaucgu uaaugaugaa     1080

aaccgagcca agguggaggu cacgccuaau aguccacgcg cagaggcuac ccuugguggc     1140

uucgggagcc ugggauugga cugugaaccc agaacaggcc ucgauuuuuc cgaccuguac     1200

uauuuaacua ugaacaauaa acacuggcug gugcauaagg agugguucca cgauauuccu     1260

cucccauggc acucuggugc cgacaccgaa acaccgcauu ggaacaauaa agaggcucuu     1320

gucgaauuua aggaugccca cgcaaagagg cagacugugg uugugcuggg gagccaggag     1380

ggcgccguac auaccgcuuu ggcgggagca cuagaggccg aaauggacgg cgcuaaaggg     1440

cggcugucaa guggacaccu caagugccgc cugaaaaugg auaaacuuag acugaaaggu     1500

gucuccuaca gcuuguguac agccgcauuc accuuuacua aggugcccgc cgagacgcuc     1560

cauggcacag uuaccgugga aguccaguau gcugggaggg acggaccuug caaggugcca     1620

gcccaaaugg caguagacau gcagacucug acccccgugg gccguuuaau aacagcgaac     1680

ccuguuauca ccgagucuac ugaaaacuca aaaaugaugc uggagcuaga uccacccuuc     1740

ggugacaguu acauugucau cggggugggc gauaagaaaa uaacacacca cuggcaucgg     1800

agcggaucca uuaucggcaa ggcuuuugag gccacggugc gaggggcaaa aagaauggcc     1860

guccucggag acaccgcuug ggauuucggu ucuguuggcg ggguguucaa uucgcuggga     1920

aagggcaucc accagauuuu uggugccgca uucaagagcc uuuuuggggg cauguccugg     1980

uucucacaaa ucuugauugg aacucugcuc guguggcugg gccuuaacac aaagaauggg     2040

aguauaucuc ugaccuguuu ggcucucgga ggcguaauga ucuuucugag cacagccguc     2100

uccgcggacg uggguugcuc aguugauuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4011
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 1 opt

<400>  4011
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucgcucugg uggccgucgc gaucgcaugg      540

uugcucggca guuccacauc ucagaaggug auuuaccugg uaaugauccu gcucauugcc      600

cccgcuuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguacuu gggucgacgu gguguuagaa cacggcgggu gugucaccgu uauggcccag      720

gauaaaccua caguggacau ugagcuggua accacgacug ugaguaacau ggcagagguc      780

cguagcuacu gcuacgaggc uucuaucucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgugugcaa aagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uucagcccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu caacauuccg guaugaucgu gaaugaugaa     1080

aaccgagcaa agguagaggu uaccccuaau ucaccacgcg cugaggccac acuuggcggg     1140

uucggaaguc ugggcuugga cugugaaccc agaacugguc ucgauuuuag cgaccuguac     1200

uauuuaacca ugaacaauaa acacuggcug gugcauaagg agugguucca cgauauuccu     1260

cucccauggc acgcuggggc cgacacaggc acuccgcauu ggaacaauaa agaagcacuu     1320

gucgaguuua aggaugccca cgcuaagagg cagaccgugg ucgugcuggg aucccaggaa     1380

ggcgcgguuc auacagcauu ggccggggcu cuagaggccg agauggacgg agcaaaaggu     1440

cggcuguucu cuggccaccu caagugccgc cugaaaaugg auaaacuuag acugaagggg     1500

gugagcuacu cauuguguac cgccgcuuuu acuuucacga agguacccgc cgaaacacuc     1560

cauggaaccg ucacugugga gguucaguau gcaggcaccg acgguccuug caaggugcca     1620

gcgcaaaugg cugucgacau gcagacacug acccccgugg ggagguuaau aacugccaac     1680

ccuguaauca cagaaaguac ggagaacucc aaaaugaugc uggaacuaga uccacccuuu     1740

ggcgacagcu acauugugau cggaguuggc gauaagaaaa uaacccacca cuggcaucgu     1800

ucugggucaa cuauuggaaa ggcauucgag gccacagucc ggggugcuaa acgaauggcc     1860

gugcucggcg acaccgcaug ggauuucggg agugugggag gcgucuuuaa uagccugggu     1920

aaggggaucc accagaucuu cggcgcugcc uuuaaguccc uuuucggagg caugucuugg     1980

uuuucgcaaa uuuugaucgg gacacugcuc guuuggcugg gacuuaacac uaaaaauggc     2040

agcauuuccc ugaccuguuu ggcgcucggu ggggugauga uauuccuguc aacggccgug     2100

agugcugacg uaggaugcuc ugucgauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4012
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 1 opt

<400>  4012
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaaa cacggcgcuc cagaaggucu gucucgcuac gguaucacua cacccgcaag      420

cuccaaacgc gaucacagac uuggcuggag agcagagagu auaagaaaca ucuuauaaug      480

guggaaaauu ggaucuuuag gaacccagga uucgcaauug uuaguguggc caucaccugg      540

cugaugggcu ccuugacauc ucagaagguc auuuaccucg ugaugaucgu acugauaguu      600

ccggcuuaca gcauuuccug caucggcgug ucaaaucggg aucuggucga ggggaugagu      660

ggagguacuu ggguggacgu gguccucgaa cacggcgggu guguuaccga gauggcccag      720

gauaaaccca caguggacau ugaguuagua accaugacgg ugagcaacau ggcggaaguc      780

cguucuuauu gcuacgaggc aucccuguca gauauggcca gcgcuagucg cuguccuacu      840

caaggagaac caucucuuga caagcagucc gacacacaga gcgugugcaa aagaaccuug      900

ggcgauaggg guugggggaa uggauguggc aucuuuggca aggggucgcu gguuacuugc      960

ucuaaguuca caugcuguaa aaagaugccc ggaaaaucca uccagccuga gaaccuagaa     1020

uaucggauua ugcucccagu gcacgguuca caacauagug gcaugauagu caaugacauc     1080

gggcacgaga ccgaugagaa ccgagccaag guggaaguaa cgcccaauag cccucgcgca     1140

gaggcuaccc ugggaggcuu ugguucccuu gggcuggacu gcgaaccaag aacaggcuug     1200

gauuucucug accucuacua ucugacuaug aacaauaaac auugguuagu ucacaaggag     1260

ugguuucacg auauuccgcu gcccuggcau gccggagcgg acaccggcac accucacugg     1320

aacaauaaag aagcccucgu ggaguucaag gaugcacaug cuaagaggca gacugucgug     1380

guccuuggga gccaggaggg agccgugcac accgcucugg ccggugcauu ggaagccgag     1440

auggacggcg cuaaagggcg gcuauuuuca ggacaucuga agugucgccu caaaauggau     1500

aagcugagac uuaaaggcgu uaguuacucc cugugcacag cggcauucac cuuuacuaag     1560

gugccagccg aaacguugca cgguacagua accgucgagg ugcagagcgc ugggacugac     1620

ggccccugua agguuccugc ccaaauggca guggacaugc agacccucac accagucgga     1680

aggcugauca ccgccaaccc cgugauaacu gaaucuacag agaacucaaa aaugauguua     1740

gagcuggauc cgccuuucgg cgacaguuau auuguaaucg gggugggaga uaagaaaauc     1800

acgcaccauu ggcaccguag cgguuccacc auuggcaagg cuuuugaagc cacuguucga     1860

ggggcaaaac ggauggcggu ccuaggagac acagcuuggg auuucggcuc uguggguggg     1920

guguucaauu cgcucggcaa gggaauccau cagauuuuug gcgccgcauu caagagccug     1980

uuugggggaa uguccugguu cucacaaaua cuuaucggca ccuugcuggu cuggcucggu     2040

cugaacacua aaaaugggag uauuucucuu acaugccugg ccuugggagg cguuaugauc     2100

uuucucagca ccgcuguguc cgccgacgug gguuguucag uagauuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4013
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 1 opt

<400>  4013
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca caaugggggu gaauaaaugc cacguccaga ucauggauuu gggacauaug      240

ugugacgcua cuaugaguua ugagugcccc auguuagaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggccauc ccgcagagcc gucacacugc cgucucacuc gaccaggaag      420

cuacaaacgc ggucacagac uuggcucgag agccgcgagu auaccaagca ucugauaaaa      480

guggaaaauu ggauccuucg aaaccccgga uuuacaguug cugcccuggc gauggcaugg      540

uugcucggca auaguacuuc ccagagagug auuuaccugg ucaugauccu gcucauugcc      600

ccugcuuauu cuaucaggug cauaggcgug agcaaccggg auuucguaga ggggaugucc      660

ggagguaccu ggguugacgu ggucuuagaa cacggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau ugagcugguu acgacuacag ugucaaauau ggcagaggua      780

cguaguuacu gcuacgaagc uagcaucucu gauauggccu ccgacucacg cugucccacc      840

caaggagagg cguaucuuga caaacagagc gauacucagu acgugugcaa gagaacauug      900

gucgacaggg gcugggguaa cgggugugga cuguuuggca aaggcagucu agugaccugc      960

gccaaguuca cgugcucuaa gaaaaugacc gggaagucca uucagccuga aaaucucgag     1020

uaucggauca ugcugagcgu ucacggaucg caacauucug guaugaucgu gaacgauauu     1080

ggccacgaaa cagacgagaa ucgagcaaaa gucgagguga cuccaaacuc accccgcgcu     1140

gaagccaccc uugggggauu uggcucccug gguuuggauu gugagccuag aacagggcuc     1200

gacuucagug aucuguacua uuuaacuaug aauaacaagc auuggcuggu acacaaagaa     1260

ugguuucacg acauaccacu cccguggcau gcuggcgccg auaccggaac accccacugg     1320

aauaacaagg aggcacuugu ugaguucaaa gacgcccaug cuaagaggca gaccgugguc     1380

gugcugggca gccaggaagg ggcgguccac acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaagggccg gcuguuuucc gggcaucuca aaugccgcau caagauggac     1500

aaacugagac uuaagggagu gucuuacagc cuguguacgg ccgcuuucac auuuucaaaa     1560

guuccugccg agaccuugca cggcacugug accguagaag uccaguaugc agguagggac     1620

gggccaugca aggugcccgc gcaaauggcu guugauaugc agacacucac cccugugggc     1680

cgucugauua cugccaaccc aaucauaaca gagaguacgg aaaauucccg gaugauguua     1740

gagcuggauc ccccguucgg agacagcuac auugucaucg gcguggggga caagaaaauc     1800

acccaccauu ggcaccgauc uggaucaacu auugguaagg cauuugaggc cacaguaaga     1860

ggcgcuaaaa ggauggccgu gcuaggggau accgcauggg acuucggaag uguuggcggu     1920

gucuucaaca gccucgggaa gggcgugcau cagguguuug gagcugccuu caaaucccug     1980

uuuggcggga ugucuugguu cucgcaaauc cuuauuggaa cauugcuggu cuggcucggc     2040

cugaauacua agaacgguag cauaucccuu gcgugucugg cccucggggg agcuaugauc     2100

uuuuugucaa ccgcaguuag ugccgaugug ggcugcucug uggacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  4014
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 1 opt

<400>  4014
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4015
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 1 opt

<400>  4015
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cgccaagaau ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaacggca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4016
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 1 opt

<400>  4016
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgguuacg agaccgacga agaucgugcg aaggucgagg ugacaccuaa cucaccacgc     1080

gccgaggcaa cucuuggcgg guucggaagu cugggcuugg acugugaacc cagaaccggu     1140

cucgauuuua gcgaccugua uuacuuaaca augaauaaca aacacuggcu gguacauaag     1200

gagugguucc acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau     1260

uggaauaaca aagaagcucu uguugaguuu aaggaugccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucccagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggacg gagcuaaggg ucggcuguuc ucuggccacc ucaagugccg acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucauugugua cugccgcauu uacguucaca     1500

aagguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gacgguccuu gcaaaauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggagauuaa uaacagcaaa uccugucauc acggaaagua ccgagaacuc caagaugaug     1680

cuggaacuag auccacccuu uggcgacagc uacauuguga ucggaguagg cgauaagaaa     1740

auaacucacc acuggcauag gucuggguca acaauuggaa aggcguucga ggcuaccgug     1800

cggggugcca aacgcauggc aguucucggc gacacagccu gggauuucgg gagugucgga     1860

ggcguguuua auagccuggg uaaggggauc caccagaucu ucggcgcugc cuuuaaaucc     1920

cuuuucggag gcaugucuug guuuucgcaa auuuugaucg ggacucugcu cguguggcug     1980

ggacuuaaca ccaagaacgg cagcauuucc cugacguguu uggcacucgg uggggucaug     2040

auauuccugu caaccgcugu uagugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4017
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 1 opt

<400>  4017
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4018
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 1 opt

<400>  4018
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgucaacg acaccgguua cgagacagau gaaaaucgug cgaaggugga gguaacuccu     1080

aacucaccac gcgccgaggc aacccuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacag gucucgauuu uagcgaccug uauuacuuaa cuaugaauaa caaacacugg     1200

cugguucaua aggagugguu ccacgauauc ccucucccau ggcacgcugg ggccgacacc     1260

ggcacaccgc auuggaauaa caaagaagcu cuuguggagu uuaaggaugc ccacgcaaaa     1320

aggcagaccg ucgugguccu gggaucccag gaaggcgccg ugcauacugc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag gguaagcugu ucucuggcca ccucaaaugc     1440

cggcugaaga uggauaaacu ucgacugaag gggguuagcu auucauugug uacggccgca     1500

uuuacauuca ccaaagugcc cgccgaaacu cuccauggaa ccguaacagu cgaggugcag     1560

uacgcuggca ccgacggucc uugcaagauu ccaguucaaa uggccgugga caugcagacu     1620

cugacacccg ucgggcgcuu aauaacggca aauccuguga ucaccgaaag uacugagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu aaucggagug     1740

ggcgauaaaa agauaacaca ccacuggcau agaucugggu caaccauugg aaaagcguuc     1800

gaggcuacag uuaggggugc caagcggaug gcaguccucg gcgacacugc cugggauuuc     1860

gggagugugg gaggcguguu uaacagccug gguaaaggga uccaccagau cuucggcgcu     1920

gccuuuaagu cccuuuucgg aggcaugucu ugguuuucgc aaauuuugau cgggacccug     1980

cucgucuggc ugggacuuaa uacgaagaac ggcagcauuu cccugaccug uuuggcacuc     2040

ggugggguua ugauauuccu gucaacagcu gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4019
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 1 opt

<400>  4019
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc gcaagaaagg ugaggcccga cguuccagaa gggcugucac acuaccaucu      360

cauucgaccc ggaagcucca aacgcgcuca cagacuuggc uggaaagcag agaguauacc      420

aaacaccuua uuaaggugga gaacuggauc uuuaggaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu cggugcaucg gcguauccaa ccgcgacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcaugg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgaag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agcagauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagga cuuuggugga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg uauuaugcug ucggugcacg ggucucaaca cuccggaaug     1020

aucgucaacg acaccgguua cgagacagau gaaaaucgcg cgaaggugga gguaacuccu     1080

aacucaccaa gagccgaggc aacccuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccaggacag gucucgauuu uagcgaccug uauuacuuaa cuaugaauaa caaacauugg     1200

cugguucaca aggagugguu ccaugauauc ccucucccau ggcacgcugg ggccgacacc     1260

ggcacaccgc acuggaauaa caaagaagcu cuuguggagu uuaaggaugc ccaugcaaaa     1320

cggcagaccg ucgugguccu gggaucccag gaaggcgccg ugcacacugc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag gguaagcugu ucucuggcca ucucaaaugc     1440

cgacugaaga uggauaaacu ucgccugaag gggguuagcu auucauugug uacggccgca     1500

uuuacauuca ccaaagugcc cgccgaaacu cuccacggaa ccguaacagu cgaggugcag     1560

uacgcuggca ccgacggucc uugcaagauu ccaguucaaa uggccgugga caugcagacu     1620

cugacacccg ucgggagauu aauaacggca aauccuguga ucaccgaaag uacugagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu aaucggagug     1740

ggcgauaaaa agauaacaca ucacuggcac aggucugggu caaccauugg aaaagcguuc     1800

gaggcuacag uucggggugc caagcgcaug gcaguccucg gcgacacugc cugggauuuc     1860

gggagugugg gaggcguguu uaacagccug gguaaaggga uccaucagau cuucggcgcu     1920

gccuuuaagu cccuuuucgg aggcaugucu ugguuuucgc aaauuuugau cgggacccug     1980

cucgucuggc ugggacuuaa uacgaagaac ggcagcauuu cccugaccug uuuggcacuc     2040

ggugggguua ugauauuccu gucaacagcu gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4020
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 1 opt

<400>  4020
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

accgucaacg acaucgguua cgagacagau gaaaaucgug cgaaggugga gguaacuccu     1080

aacucaccac gcgccgaggc aacccuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacag gucucgauuu uagcgaccug uauuacuuaa cuaugaauaa caaacacugg     1200

cugguucaua aggagugguu ccacgauauc ccucucccau ggcacgcugg ggccgacacc     1260

ggcacaccgc auuggaauaa caaagaagcu cuuguggagu uuaaggaugc ccacgcaaaa     1320

aggcagaccg ucgugguccu gggaucccag gaaggcgccg ugcauacugc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag gguaagcugu ucucuggcca ccucaaaugc     1440

cggcugaaga uggauaaacu ucgacugaag gggguuagcu auucauugug uacggccgca     1500

uuuacauuca ccaaagugcc cgccgaaacu cuccauggaa ccguaacagu cgaggugcag     1560

uacgcuggca ccgacggucc uugcaagauu ccaguucaaa uggccgugga caugcagacu     1620

cugacacccg ucgggcgcuu aauaacggca aauccuguga ucaccgaaag uacugagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu aaucggagug     1740

ggcgauaaaa agauaacaca ccacuggcau agaucugggu caaccauugg aaaagcguuc     1800

gaggcuacag uuaggggugc caagcggaug gcaguccucg gcgacacugc cugggauuuc     1860

gggagugugg gaggcguguu uaacagccug gguaaaggga uccaccagau cuucggcgcu     1920

gccuuuaagu cccuuuucgg aggcaugucu ugguuuucgc aaauuuugau cgggacccug     1980

cucgucuggc ugggacuuaa uacgaagaac ggcagcauuu cccugaccug uuuggcacuc     2040

ggugggguua ugauauuccu gucaacagcu gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4021
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 1 opt

<400>  4021
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua ucaagguuga gaacuggauu uuuagaaauc cgggguucac acugguggca      480

gucgccaucg cuugguugcu cggaaguucc acuucucaga aggugauaua ccugguaaug      540

auccugcuca uugcccccgc guauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aaccuggguc gauguggugu uagagcacgg uggcuguguc      660

acaguuaugg cacaggacaa accuaccgug gauaucgaac ugguaacgac uacagugagu      720

aauauggccg agguccgcag cuacugcuac gaggcuucua uauccgacau ggccucagau      780

agccgauguc caacccaagg ggaagcauau cuugacaagc agagugacac ucaguacgug      840

ugcaaaagaa cauugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga ccugcgcuaa auuuacgugc uccaagaaga ugaccggcaa aagcauucag      960

cccgagaauc ucgaauaucg gaucaugcug ucgguccacg ggucucaaca uuccggaaug     1020

auugugaacg acgagaaucg ugccaaggua gaaguuacac cuaacucacc acgcgcggag     1080

gccacucuug guggcuucgg gagucuggga uuggauugug agcccagaac cggccucgac     1140

uuuagcgauc uguacuauuu aacaaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaca ucccucuccc auggcacgca ggggcugaua cugggacccc gcauuggaau     1260

aacaaagagg cccuugucga auuuaaggac gcucacgcca aaaggcagac aguggucgug     1320

cugggcuccc aggagggagc aguucauacc gccuuggcug gcgcgcuaga agcagagaug     1380

gauggggcca agggacggcu guucucuggu caccucaagu gccgacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucauug uguacugcug ccuuuacguu cacaaaggua     1500

cccgcagaga cccuccaugg gacugucacc guggaaguuc aguaugccgg aacagauggc     1560

ccuugcaaag ugccagcuca aauggccguc gacaugcaga cccugacucc cguggguaga     1620

uuaaucacag caaauccugu aauuacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagacccac ccuuugggga uagcuacaua gugaucggcg uuggagacaa gaaaauuacu     1740

caccacuggc auaggucugg cucaacaauc gggaaggcgu ucgaagcuac cguccgggga     1800

gccaaacgca uggcagugcu cggugauaca gccugggacu ucggcagugu ggguggaguc     1860

uuuaacagcc ugggcaaggg uauacaccag auuuucgggg cugccuuuaa aucccuuuuc     1920

ggcggaaugu cuugguuuuc gcaaaucuug aucggcacuc ugcucguuug gcuggggcuu     1980

aauaccaaga acggaagcau uucccugacg uguuuggcac ucggcggugu gaugaucuuc     2040

cugucaaccg cugugagugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4022
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 1 opt

<400>  4022
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgagaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgguuacg agaccgacga aaaccgugcg aaggucgagg ugacaccuaa uucaccacgc     1080

gccgaggcaa cucuuggcgg guucggaagu cugggcuugg auugugaacc cagaaccggu     1140

cucgacuuua gcgaucugua uuacuuaaca augaacaaua aacacuggcu gguacauaag     1200

gagugguucc acgacauccc ucucccaugg cacgcugggg ccgauacugg caccccgcau     1260

uggaacaaua aagaagcucu uguugaguuu aaggacgccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucccagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggaug gagcuaaggg uaagcuguuc ucuggccacc ucaaaugccg gcugaagaug     1440

gacaaacuuc gacugaaggg ggugagcuau ucauugugua cugccgcauu uacguucaca     1500

aaaguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gaugguccuu gcaagauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggcgcuuaa uaacagcaaa cccugucauc acggaaagua ccgaaaauuc caagaugaug     1680

cuggaacuag acccacccuu uggcgauagc uacauuguga ucggaguagg cgacaaaaag     1740

auaacucacc acuggcauag aucuggguca acaauuggaa aagcguucga ggcuaccgug     1800

aggggugcca agcggauggc aguucucggc gauacagccu gggacuucgg gagugucgga     1860

ggcguguuua acagccuggg uaaagggauc caccagaucu ucggcgcugc cuuuaagucc     1920

cuuuucggag gcaugucuug guuuucgcaa auuuugaucg ggacucugcu cguguggcug     1980

ggacuuaaca ccaagaaugg cagcauuucc cugacguguu uggcacucgg uggggucaug     2040

auauuccugu caaccgcugu uagugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4023
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 1 opt

<400>  4023
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaggggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaaaaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4024
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 1 opt

<400>  4024
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccgggcuggc uuuggcagcc      480

gcugccauug cguggcuccu gggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacucuuaa ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuc      600

guagagggca uguccggggg aacuuggguu gauguggucc uggaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc uuguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac uuggacaagc agagcgacac ccaguaugug      840

ugcaaaagga cucuggucga ucggggaugg ggcaaugguu gugggcuauu uggaaagggc      900

agucucguga caugcgcgaa auucgccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc uggaguaccg uauuaugcuu agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucugggc ggguuuggau cauugggccu cgacugugag     1140

ccuaggaccg gucuggauuu cagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ucacgauauc ccacuuccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cugguagagu ucaaggaugc ccacgcuaaa     1320

cggcagacag uuguggucuu gggaagccag gaaggcgcgg ugcauaccgc acuagccggg     1380

gcucuggagg ccgaaaugga cggagcaaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gagauugaaa ggggucagcu auucacucug uacugccgcu     1500

uuuacguuca caaagauccc ugccgagacc cugcauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

uuaacuccug uggggaggcu gauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgaucccccg uuuggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuc     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacugg gcgauacagc augggacuuu     1860

gggagugugg gaggcgcucu uaauagcuug gguaagggga uucaccagau cuucggcgcc     1920

gcguucaaau cccuguuugg aggcaugucu ugguucucgc aaauccucau ugggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcaucu cccucaugug ucuggccuua     2040

ggugggguuc ugauuuuucu aucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4025
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 1 opt

<400>  4025
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacugguau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaucagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4026
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 1 opt

<400>  4026
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga cacccagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cacgaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4027
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 1 opt

<400>  4027
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agauccgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4028
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 1 opt

<400>  4028
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cuggcgcaaa uggcugucga caugcagacc     1620

uuaacucccg uggggaggcu gauuacagcc aacccuguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgauccaccc uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacugg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu uaauagcuug gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cccuguucgg aggcaugucu ugguuuucgc aaauccucau ugggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcaucu cccucaugug ucuggccuua     2040

ggugggguuc ugauuuuccu aucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4029
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 1 opt

<400>  4029
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuacccu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cacgaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4030
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 1 opt

<400>  4030
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacgguc gugagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4031
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 1 opt

<400>  4031
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa gugcaugggu ggucuauggc      300

accugucauc acaaaaaggg ugaagcccgc cgaagccgua gagcugugac ucuaccgucc      360

cauucuacaa ggaaacucca aacccggccc cagacguggc uggagucgcg cgaguacacu      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc cuggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaucaagc accagucaga aagugaucua ucuggucaug      540

auacugcuca ucgccccagc uuacuccauu cggugcaucg gcgugucuaa ucgcgacuuu      600

guagagggca ugagcggggg aacauggguu gauguggucu uagaacaugg uggcugugug      660

acugugaugg cccaggacaa gcccaccguc gauauugagc ugguuacaac cacggugucc      720

aacauggcag agguacgauc auacugcuau gaagcuagua ucagcgacau ggccucugau      780

uccagauguc cuacucaagg ggaggcguac cuugacaagc agucagacac acaguaugug      840

ugcaaaagga ccuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agccuaguga cuugcgccaa auuugcaugc aguaagaaaa ugacaggcaa gucuauacag      960

ccagaaaacc ucgaguaccg uauuaugcug uccguucacg ggagccaaca cucgggaaug     1020

aucgugaaug acaccgguca ugaaacggau gagaaccgcg cuaaggucga gauuaccccc     1080

aauucuccua gagccgaagc uacacuuggc ggguucggau cccugggcuu ggacugugag     1140

ccaaggacug gucucgauuu uucagaccug uauuacuuaa ccaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccgcuccccu ggcacgccgg ggcagacaca     1260

ggcacuccuc auuggaacaa uaaagaggcc cuuguagaau uuaaggaugc ucacgcgaaa     1320

cggcagaccg uugugguccu gggaagucag gagggcgcag ugcauacagc cuuggcuggg     1380

gcccuagagg cagaaaugga cggagccaag ggucgacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaaa ggggucucuu auagcuugug uaccgcugcc     1500

uucacuuuua cgaagauccc agcagagaca cuccauggaa ccgugacugu ugaagugcag     1560

uacgcgggca ccgacggucc cugcaaagua ccugcucaaa uggccgucga caugcagaca     1620

cugaccccag uggggagguu aauuacugca aaccccguua uaacagaguc aacggaaaac     1680

aguaagauga ugcuggagcu agauccgccu uucggcgacu ccuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggagcgggu cuacuauagg aaaggccuuu     1800

gaagcuacag ugcgcggugc caaaagaaug gcaguacucg gcgauaccgc uugggacuuc     1860

gggucagugg gaggcgcccu gaauagucuu gguaagggga uucaccagau cuucggcgcg     1920

gccuuuaaga gcuuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcucgauca gcuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu guccaccgca gucucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4032
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 1 opt

<400>  4032
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acauccaaaa aggugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaggg ggaggccuac cuugacaaac aaagcgacac ccaguaugug      840

ugcaagagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaaaggc      900

agucuaguga caugcgcgaa guuugccugc ucuaaaaaga ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcagca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaagucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaagcacugg     1200

cuggugcaua aagaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaggaggca cuuguagaau uuaaagaugc ccacgcuaag     1320

cggcaaacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaaa ggucgacugu ccucuggcca ccucaagugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaaaauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaggua cccgcgcaga uggcugucga caugcaaacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaga agaucacuca ccacuggcau cggucugggu caacaauagg aaaagcauuu     1800

gaagccaccg ugcgcggugc uaagagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaaaggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc agauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaaaaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4033
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 1 opt

<400>  4033
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acacggcaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacuccauu cgcugcaucg gcgugucuaa ucgagacuuu      600

guagagggca ugagcggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugucc      720

aacauggccg agguaagauc auacugcuau gaagcaagua ucagcgacau ggcuucugau      780

uccagguguc ccacacaagg ggaggccuac cuugacaagc agucagacac ccaguaugug      840

ugcaaacgga cuuuggucga ucguggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agccuaguga caugcgcgaa auuugccugc aguaagaaaa ugaccggcaa gucuauacag      960

ccugaaaacc ucgaguaccg cauuaugcug uccguucacg ggagccaaca cucgggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaacagag caaaggucga gauuacacca     1080

aauucuccca gggcugaagc cacucuuggc ggguucggau cccugggcuu ggacugugag     1140

ccucggaccg gucucgauuu uucagaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cgacagacag uugugguccu gggaagucag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgccuga gcuccggcca ccucaaaugc     1440

agacugaaga uggauaaacu uaggcugaaa ggggucucuu auagcuugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggcgguu aauuacagcc aacccaguua uaacggaguc aaccgaaaac     1680

aguaagauga ugcuggagcu agaucccccg uucggcgacu ccuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cgcagcgggu cuacaauagg aaaggcauuu     1800

gaagccaccg ugagaggugc uaaaaggaug gccguacucg gcgauacagc augggacuuc     1860

gggucagugg gaggcgcucu gaauagucuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaga gcuuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcucgauca gcuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu guccacggcu gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4034
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 1 opt

<400>  4034
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaacccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaaa ggggucucuu auagcuugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgaguc aacugaaaac     1680

aguaagauga ugcuggagcu agaucccccg uucggcgacu ccuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggagcgggu cuaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggucagugg gaggcgcucu gaauagucuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaga gcuuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cacgaagaau ggcucgauca gcuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu guccaccgcu gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4035
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 1 opt

<400>  4035
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuuua cggugcauug gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucc uggaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauaucgagc uuguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca uuucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac uuggacaagc agagcgacac ccaguaugug      840

ugcaaaagga cucuggucga ucggggaugg ggcaaugguu gugggcuauu cggaaagggc      900

agucucguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauccag      960

ccugaaaacc uggaguaccg uauaaugcuu agcguucacg ggucgcaaca cucuggaaug     1020

auugugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gaucacacca     1080

aauuccccca gagcugaagc cacucugggc ggguucggau cauugggccu cgacugugag     1140

ccuaggaccg gucuggauuu uagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccacuuccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cugguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uuguggucuu gggaagccag gagggcgcgg ugcauaccgc acuagccggg     1380

gcucuggagg ccgaaaugga cggagcaaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gagauugaaa ggggucagcu auucacucug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cugcauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

uuaacuccug uggggaggcu gaucacagcc aacccaguua uuacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgaucccccg uucggcgaca gcuacauagu gaucggaguc     1740

ggcgagaaaa agauuacuca ccacuggcau cggucugggu caacaaucgg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacugg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu uaauagcuug gguaagggga uacaccagau uuucggcgcc     1920

gcguuuaaau cccuguucgg aggcaugucu ugguuuucgc aaauccucau cgggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcauuu cccucaugug ucuggccuua     2040

ggugggguuc ugaucuuccu aucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4036
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 1 opt

<400>  4036
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaaucga aaauuggauu uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccaucg cgugguugcu cggaagcagu acaucccaga aaguuauaua ucuggugaug      540

auccugcuca uugcacccgc cuacucuauc cggugcauug gcgucagcaa ucgcgacuuu      600

guggagggca uguccggggg aacuugggua gauguugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4037
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 1 opt

<400>  4037
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc cugaggaacc ccggguuugc uuuggcagcc      480

gcugccauug cguggcuccu gggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacucuuaa ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuc      600

guagagggca uguccggggg aacuuggguu gauguggucc uggaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc uuguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac uuggacaagc agagcgacac ccaguaugug      840

ugcaaaagga cucuggucga ucggggaugg ggcaaugguu gugggcuauu uggaaagggc      900

agucucguga caugcgcgaa auucgccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc uggaguaccg uauuaugcuu agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucugggc ggguuuggau cauugggccu cgacugugag     1140

ccuaggaccg gucuggauuu cagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ucacgauauc ccacuuccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cugguagagu ucaaggaugc ccacgcuaaa     1320

cggcagacag uuguggucuu gggaagccag gaaggcgcgg ugcauaccgc acuagccggg     1380

gcucuggagg ccgaaaugga cggagcaaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gagauugaaa ggggucagcu auucacucug uacugccgcu     1500

uuuacguuca caaagauccc ugccgagacc cugcauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

uuaacuccug uggggaggcu gauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgaucccccg uuuggcgaca gcuacaucgu gauuggaauc     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuc     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcugg gcgauacagc augggacuuu     1860

gggaguguag gaggcgcucu uaauagcuug gguaagggga uccaccagau cuucggcgcc     1920

gcguucaaau cccuguuugg aggcaugucu ugguucucgc aaauucucau cgggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcauuu cccucaugug ucuggccuua     2040

gguggggugc ugauauuucu aucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4038
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 1 opt

<400>  4038
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuucaac cacggugagu      720

aacauggccg agguacgaag cuacugcuau gaagcaacua ucucugacau agcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcguucacg ggucgcaaca cucuggaaug     1020

auugugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag cgguugugcu gggaagccag gagggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagaaaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaagcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4039
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 1 opt

<400>  4039
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaacugcag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

uuaacuccug ucgggaggcu gauuacagcc aacccaguga uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgaucccccg uucggcgaca gcuacaucgu uauuggagug     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcugg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu uaauagcuug gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cccuguucgg aggcaugucu ugguuuucgc aaauccucau ugggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcaucu cccucaugug ucuggccuua     2040

gguggggugc ugauuuuccu aucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4040
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 1 opt

<400>  4040
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaucgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu gaucggaguc     1740

ggcgagaaaa agauaaccca ccacuggcau cggucugggu caacuauugg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauaccgc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

ggugggguuu uaauauuccu gucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4041
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 1 opt

<400>  4041
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagucaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaguu aagacugaaa ggggugagcu auucauugug uacugcagcc     1500

uucacguuua caaagauccc ugcugagacc cuccauggaa cuguuaccgu ggaaguacag     1560

uacgccggca cagacggucc augcaaaguc cccgcacaaa uggcggugga caugcagacc     1620

cugacuccug uugggagguu aauuacagcu aacccaguga uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu cauuggagug     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggccuuu     1800

gaagcaaccg uacgcggugc caaaagaaug gcugugcucg gcgauacagc cugggacuuc     1860

gggaguguug gaggcgcacu gaauagccuu gguaagggga uucaccagau cuucggcgcu     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuucaugug uuuggcgcuc     2040

gguggggucc ugauuuuucu uucaacggcc gugagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4042
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 1 opt

<400>  4042
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag gguaaacugu ccucuggcca ccucaagugc     1440

cgacugaaga uggauaaacu ucgccugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaaaauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaggua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagauu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaga agaucacuca ccacuggcau aggucugggu caacaauagg aaaagcauuu     1800

gaagccaccg ugcggggugc uaagcgcaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaaaggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaaaaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4043
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 1 opt

<400>  4043
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaaggaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaca aaugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa guaccugggu ggucuauggc      300

acuugucauc acaagaaagg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaagcucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aaacaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aggugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ccgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aauauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaaac agagcgacac ccaguaugug      840

ugcaagagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaaaggc      900

agucuaguga caugcgcgaa guuugccugc ucuaaaaaga ugaccggcaa guccauacag      960

ccugaaaauc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaagucga gauuacacca     1080

aacuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaauaa caagcacugg     1200

cuggugcaua aagaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaggaggca cuuguagaau uuaaagaugc ccacgcuaag     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaaa ggucgacugu ccucuggcca ccucaagugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaaaauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaggua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aauccaguua uaacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaga agaucacuca ccacuggcau cggucugggu caacaauagg aaaagcauuu     1800

gaagccaccg ugcgcggugc uaagagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaacagccuu gguaaaggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa uaccaaaaac ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4044
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 1 opt

<400>  4044
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaggc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagaag ccgagaugga uggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggacaaacu uagacugaaa ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgaaacc cuccauggaa cugugaccgu ugaggugcag     1560

uacgcaggca cagauggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggaaag uaccgagaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgaaaaga agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaggccaccg ugcgcggugc uaaaagaaug gccguacucg gcgacacagc augggauuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4045
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 1 opt

<400>  4045
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cgcucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacug gucucgauuu uagugaccug uauuacuuaa ccaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacaca     1260

ggcacucccc auuggaacaa uaaagaggcc cuuguagaau uuaaggaugc ucacgcgaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcag ugcauacagc cuuggcuggg     1380

gcccuagagg cagaaaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uaccgcugcc     1500

uucacuuuua cgaagauccc ugcagagaca cuccauggaa ccgugacugu ugaagugcag     1560

uacgcgggca ccgacggucc augcaaagua cccgcucaaa uggccgucga caugcagaca     1620

cugaccccug uggggagguu aauuacugca aacccaguua uaacagagag uacggaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggucugggu caacuauagg aaaggccuuu     1800

gaagcuacag ugcgcggugc caaaagaaug gcaguacucg gcgauaccgc uugggacuuc     1860

gggagugugg gaggcgcccu gaauagccuu gguaagggga uucaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaacacugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

ggugggguuu uaaucuuccu gucaacggca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4046
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 1 opt

<400>  4046
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggcg cggacggucc augcaaagua cccgcucaaa uggccgucga caugcagaca     1620

cugaccccug uggggagguu aauuacugca aacccaguua uaacagagag uacggaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggucugggu caacuauagg aaaggccuuu     1800

gaagcuacag ugcgcggugc caaaagaaug gcaguacucg gcgauaccgc uugggacuuc     1860

gggagugugg gaggcgcccu gaauagccuu gguaagggga uucaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4047
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 1 opt

<400>  4047
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcauuagc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucc uggaacaugg uggcugugug      660

accgugaugg cucaggacaa gccuacaguc gauauugagc uuguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc caacacaagg ggaggccuac uuggacaagc agagcgacac ccaguaugug      840

ugcaaaagga cucuggucga ucggggaugg ggcaaugguu gugggcuauu cggaaagggc      900

agucucguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

cccgaaaacc uggaguaccg uauuaugcuu agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacaccu     1080

aauuccccaa gagcugaagc cacucugggc ggguucggau cauugggccu cgacugugag     1140

cccaggaccg gucuggauuu uagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauc ccucuuccau ggcacgcugg ggccgacacu     1260

ggcaccccgc auuggaacaa uaaagaggca cugguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uuguggucuu gggaagccag gagggcgcgg ugcauaccgc acuagccggg     1380

gcucuggagg ccgaaaugga cggagcaaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gagauugaaa ggggucagcu auucacucug uacugccgcu     1500

uucacguuua caaagauccc cgccgagacc cugcauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc uugcaaagua ccagcgcaaa uggcugucga caugcagacc     1620

uuaacucccg uggggaggcu gauuacagcc aacccuguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgauccaccc uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacugg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu uaauagcuug gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cccuguucgg aggcaugucu ugguuuucgc aaauccucau ugggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcaucu cccucaugug ucuggccuua     2040

ggugggguuc ugauuuuccu aucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4048
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 1 opt

<400>  4048
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau gguguccgau      780

ucaagauguc ccacacaagg ggaggcuuac cuugacaagc agagcgacac ccaguauguc      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguua caugcgccaa auuugcgugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

aucgucaaug acacggguca ugaaaccgau gagaaccgcg ccaaggugga gauuacacca     1080

aauuccccca gagcagaagc uacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguacaua aggaaugguu ccacgauauc ccacucccgu ggcacgccgg ggcugacacu     1260

ggcaccccuc auuggaacaa uaaagaggcc cuuguugaau uuaaggaugc acacgccaaa     1320

cggcagacag uggucgugcu gggaagccag gagggcgcug uccauaccgc guuggcaggg     1380

gcccuagagg cugaaaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcagcc     1500

uucacguuua caaagauccc ugcugagacc cuccauggaa cuguuaccgu ggaaguacag     1560

uacgccggca cagacggucc augcaaaguc cccgcacaaa uggcggugga caugcagacc     1620

cugacuccug uugggagguu aauuacagcu aacccaguga uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu cauuggagug     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggccuuu     1800

gaagcaaccg uacgcggugc caaaagaaug gcugugcucg gcgauacagc cugggacuuc     1860

gggaguguug gaggcgcacu gaauagccuu gguaagggga uucaccagau cuucggcgcu     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcgcug     2040

gguggggucu uaauuuuccu gucaacggcc gugagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4049
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 1 opt

<400>  4049
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcauuagcaa ucgcgacuuu      600

guggagggca uguccggggg aacuugggua gauguugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggaa uccugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uucagaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauu ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagucag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaaa ggggugucuu auagcuugug uacugccgcu     1500

uucacguuua caaagauacc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aaucacagcc aacccaguga uuacggaguc aaccgaaaac     1680

aguaagauga ugcuggagcu agaucccccg uucggcgacu ccuacaucgu uauaggagug     1740

ggcgagaaaa agauuacuca ccacuggcau cggagcgggu cuacaaucgg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggucaguag gaggcgcucu gaauagucuu gguaagggga uccaccagau uuucggcgcc     1920

gcguuuaaga gcuuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcucgauca gcuugaugug ucucgcccug     2040

gguggggugu uaauauuccu guccacggcu guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4050
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 1 opt

<400>  4050
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuauua ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua ucagaguuga aaauuggauu uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccaucg cgugguugcu cggaagcagu acaucccaga aagugauaua ucuggucaug      540

auccugcuca uugcacccgc cuacucuauc cggugcauug gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauaucgagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcguucacg ggucgcaaca cucuggaaug     1020

auugugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauaacagcc aacccaguua ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu gaucggaguc     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa cgccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

ggugggguuu uaauauuccu gucaaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4051
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 1 opt

<400>  4051
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacaa cgcagccgac gugccgugac acuaccgucc      360

cauucuacca gaaaacucca gacgaggucg cagacuuggc uggagucacg ggaguacacc      420

aagcaccuua uucgcguuga aaauuggauc uuuagaaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu aggugcaucg gcgugagcaa ucgggacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaagacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgcag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucacgauguc ccacacaggg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagaa cuuuggucga uaggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacaa      960

ccugaaaacc ucgaguaccg gauuaugcug agcguucacg ggucgcagca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgug caaaggucga gauuacacca     1080

aauuccccuc gcgcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuagaaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

aggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucggcugu ccucuggcca ccucaaaugc     1440

cgacugaaga uggauaaacu ucgccugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcaa     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaga uggcugucga caugcagacc     1620

cugacuccug uggggagauu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau aggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcggggugc uaaacgcaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccaaau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc agauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4052
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 1 opt

<400>  4052
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca agcucggucg cagacguggc uggagucacg cgaguacacu      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu accucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacauggguu gauguggucu uagaacaugg uggcugugug      660

acugugaugg cccaggacaa gccaaccguc gauauugagc ugguuacaac cacgguguca      720

aacauggcag agguacgaag uuacugcuau gaagcuagca ucucugacau ggccuccgau      780

ucaagauguc ccacucaagg ggaggcguac cuugacaagc agagcgacac acaguaugug      840

ugcaaaagga ccuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga cuugcgccaa auuugcaugc ucuaagaaaa ugacaggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacggau gagaaccgcg cuaaggucga gauuacccca     1080

aauuccccca gagccgaagc uacacuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacug gucucgauuu uagugaccug uauuacuuaa ccaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacaca     1260

ggcacucccc auuggaacaa uaaagaggcc cuuguagaau uuaaggaugc ucacgcgaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcag ugcauacagc cuuggcuggg     1380

gcccuagagg cagaaaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uaccgcugcc     1500

uucacuuuua cgaagauccc ugcagagaca cuccauggaa ccgugacugu ugaagugcag     1560

uacgcgggca ccgacggucc augcaaagua cccgcucaaa uggccgucga caugcagaca     1620

cugaccccug uggggagguu aauuacugca aacccaguua uaacagagag uacggaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggucugggu caacuauagg aaaggccuuu     1800

gaagcuacag ugcgcggugc caaaagaaug gcaguacucg gcgauaccgc uugggacuuc     1860

gggagugugg gaggcgcccu gaauagccuu gguaagggga uucaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4053
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 1 opt

<400>  4053
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacagcauu cggugcaucg gcguguccaa ccgcgacuuu      600

guagagggca ugucaggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugagu      720

aauauggccg agguacgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacacaagg ggaggccuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcgaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacucaccca gagcugaagc cacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaucccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaaa ggggucucau auaguuugug uacugccgcu     1500

uucacguuuu ccaagauccc ugccgagaca cuccauggaa ccgugacuga agagguucag     1560

uacgcaggca ccgacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagaca     1620

cugaccccug ucgggagguu aauuacugcc aauccaguga uaacagaaag cacggagaac     1680

ucuaagauga ugcuggagcu agaucccccg uucggcgacu cauacaucgu uauuggagug     1740

ggcgaaaaga agaucaccca ccacuggcau cggaguggga gcacuauagg aaaggcauuu     1800

gaggccacag uccgcggugc uaaaagaaug gccgugcucg gcgauaccgc augggacuuc     1860

ggguccguag gaggcgcucu gaacucucuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cguuguucgg aggcaugagc ugguuuuccc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa uacuaagaac ggcucaauca guuugaugug ucucgcccug     2040

gguggggugu uaauuuuccu gucuaccgcu guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4054
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 1 opt

<400>  4054
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacagcauu cggugcaucg gcguguccaa ccgcgacuuu      600

guagagggca ugucaggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugagu      720

aauauggccg agguacgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacacaagg ggaggccuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcgaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacucaccca gagcugaagc cacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacacugg ggcugacacc     1260

ggcacacccc auuggaauaa caaagaggcc cuuguagaau uuaaggaugc acacgccaaa     1320

cggcagaccg uugugguccu gggaucccag gagggcgcug ugcauacugc guuggcaggg     1380

gcccuagagg cugaaaugga cggagccaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gagacugaaa ggggucucau auaguuugug uacggcagcc     1500

uucacauuua ccaagauccc ugcugagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacggucc augcaaagua cccgcacaaa uggcggucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcu aauccaguua uaaccgaggg cacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggagaca gcuacaucgu gauuggcguc     1740

ggggagaaaa agaucacaca ccacuggcau cggucuggau caaccauagg uaaggccuuu     1800

gaagcaacag ugcgcggcgc caaaagaaug gcuguacucg gggauacugc cugggacuuc     1860

ggaagugugg gcgguguucu gaacagccuu gggaagggca uucaccagau cuucggagca     1920

gcuuuuaaau ccuuguucgg cgggaugucu ugguuuucgc aaauccugau uggaacccuc     1980

cugauguggc uuggccugaa uacgaagaac gguagcaucu ccuugaugug ucucgcccuu     2040

gggggagucu uaauuuuccu gucaaccgcg gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4055
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 1 opt

<400>  4055
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacagcauu cggugcaucg gcguguccaa ccgcgacuuu      600

guagagggca ugucaggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugagu      720

aauauggccg agguacgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacacaagg ggaggccuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcgaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacucaccca gagcugaagc cacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaucccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucucau auaguuugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aauccaguua uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucaggga guacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagcgugg gaggcgcucu gaacucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa uaccaagaac ggcuccaucu cauugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gaguacggcu gucucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4056
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 1 opt

<400>  4056
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggaaacgca uacuauaugu accuggaccg guccgaugcc      120

ggugaagcga uuucuuuccc caccacaaug ggcaugaaua agugcuauau acagaucaug      180

gacuuggggc acauguguga ugcuacuaug ucauacgagu gcccuauguu agacgaagga      240

guagagccag augacgugga uuguuggugc aacaccacaa guaccugggu cguguauggc      300

acuugucauc acaaaaaggg ugaagcccgc cgaagccgua gagcaguuac acugccgucc      360

cauucuacca ggaaacuaca aacgcggucg cagacuuggc ucgagucacg cgaguacacc      420

aagcaccuga uuagagugga aaauuggauc uuuaggaacc ccggguucgc ccuugcugca      480

gccgcuauug ccuggcuguu gggaagcagu acaucccaga aagucaucua ucucgugaug      540

auacugcuga ucgcgccugc auacucuauu cggugcaucg gcguaagcaa ucgcgacuuu      600

guugagggca uguccggggg aacuugggug gaugucgugc ucgaacaugg uggcugugug      660

accgucaugg cccaggacaa gccaacaguu gauauugagu uagugaccac gacuguauca      720

aacauggcug aggugcgaag uuacugcuau gaagccagca ucucugacau ggcauccgau      780

ucaagauguc ccacacaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguc      840

ugcaaaagga cucuugugga ucggggaugg ggcaaugguu guggguuguu cggaaagggc      900

agucugguua caugcgccaa auuugcgugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc uagaguaccg uauuaugcuc agcgugcacg ggucgcaaca cucuggaaug     1020

aucgucaaug acacggguca ugaaaccgau gagaaccgcg ccaaggugga gauuacacca     1080

aauuccccca gagcagaagc uacucugggc ggguucggau cacuuggccu ggacugugag     1140

ccuaggaccg guuuggauuu uagugaccuc uauuaccuga caaugaacaa uaaacacugg     1200

uuaguacaua aggaaugguu ccacgauauc ccacugccgu ggcacgccgg ggcugacacu     1260

ggcacacccc auuggaacaa uaaagaggcc cucguugaau uuaaggaugc acacgccaaa     1320

cggcagaccg uggucgugcu uggaagccag gagggcgcug uccauaccgc gcuggcaggg     1380

gccuuggagg cugaaaugga cggagccaag ggucgacuau ccucuggcca ccugaaaugc     1440

cgccucaaga uggauaagcu gagacuuaaa ggggugagcu auucacugug uacugcagcc     1500

uucacguuua caaagauccc ugcugagacc uugcauggaa cuguuaccgu ggaaguacag     1560

uacgccggca cagacggucc augcaaaguc cccgcacaaa uggcggugga caugcagacc     1620

cucacuccug uugggaggcu gauuacagcu aacccaguga uaacggagag uaccgaaaac     1680

uccaagauga uguuagagcu ggaucccccg uucggcgaca gcuacaucgu cauuggagug     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggccuuu     1800

gaagcaaccg uacgcggugc caaaagaaug gcugugcuag gcgauacagc cugggacuuc     1860

gggaguguug gaggcgcacu caauagccug gguaagggga uucaccagau cuucggcgcu     1920

gccuuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaaucuugau ugggacucug     1980

cucgucuggc ugggacuuaa caccaagaau ggcagcaucu cccugaugug uuuggcgcuc     2040

gguggggugc ugauuuucuu aucaacggcc gugagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4057
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 1 opt

<400>  4057
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacagcauu cggugcaucg gcguguccaa ccgcgacuuu      600

guagagggca ugucaggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugagu      720

aauauggccg agguacgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacacaagg ggaggccuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcgaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacucaccca gagcugaagc cacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaucccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucucau auaguuugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aauccaguua uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucaggga guacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagcgugg gaggcgcucu gaacucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa uaccaagaac ggcuccaucu cauugguuug ucucgcccug     2040

gguggggucu uaauuuuccu gaguacggcu gugucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4058
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 1 opt

<400>  4058
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacagcauu cggugcaucg gcgugaacaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugguuug ucucgcccug     2040

gguggggucu uaauuuuccu gucaacggcu gugagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4059
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 1 opt

<400>  4059
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggauccgca uacuauaugu accuggaccg gucugaugcc      120

ggugaagcga uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcuacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guagagccag augacgugga uuguuggugc aauaccacaa gcaccugggu cguguauggc      300

acuugucauc acaaaaaggg ugaagcccgc cgaucccgua gagcaguuac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagagugga aaacuggauc uuuaggaauc ccggguucgc ccuggcugca      480

gccgcuauug ccugguugcu cggaaguucc acaucucaga aagucaucua ucuggugaug      540

auacugcuca ucgcgccugc auacagcauu cggugcaucg gcguauccaa ccgcgacuuu      600

guugagggca ugucaggggg aacuugggug gaugucgugu uagaacaugg uggcugugug      660

accgucaugg cccaggacaa gccagcuguu gauauugagc uggugacaac cacgguaagu      720

aauauggccg aggugcgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacucaagg ggaggccuac cuugacaagc agagugacac acaguauguc      840

ugcaaaagga ccuuggugga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua cuugcgcgaa auuugccugc uccaagaaaa ugacaggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucggugcacg ggucucaaca cuccggaaug     1020

aucgucaacg acaccgguca ugaaacggau gagaaucgcg caaaggugga gauuacccca     1080

aacucaccca gagcugaagc cacacuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggacug gucucgauuu uagcgaccug uauuacuuaa ccaugaauaa caaacacugg     1200

cugguacaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacaca     1260

ggcacucccc auuggaauaa caaagaggca cuuguugaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uggucgugcu gggaucccag gagggcgcgg uccauacagc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugucau auaguuugug uaccgccgcu     1500

uucacuuuua cgaagauccc ugccgagaca cuccauggaa ccguuacugu ggaaguacag     1560

uacgcaggca ccgacggucc augcaaaguc cccgcgcaaa uggcugugga caugcagaca     1620

cugaccccug uugggagguu aauuacugcc aauccaguga uaacagaguc cacggaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu cauuggagug     1740

ggcgagaaaa agaucaccca ccacuggcau cggucaggga guacuauagg aaaggcauuu     1800

gaagccacag uacgcggugc uaaaagaaug gccgugcucg gcgauaccgc augggacuuc     1860

gggagcguug gaggcgcucu gaacucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacacuc     1980

cuggucuggc uuggacugaa uacuaagaac ggcuccaucu cauugaccug ucucgcccug     2040

gguggggugu uaauuuuccu gaguacggcu gugucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4060
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 1 opt

<400>  4060
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggauccgca uacuauaugu accuggaccg gucugaugcc      120

ggugaagcga uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcuacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guagagccag augacgugga uuguuggugc aauaccacaa gcaccugggu cguguauggc      300

acuugucauc acaaaaaggg ugaagcccgc cgaucccgua gagcaguuac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagagugga aaacuggauc uuuaggaauc ccggguucgc ccuggcugca      480

gccgucauug cuugguugcu cggaaguucc acaucucaga aagugaucua ucugguaaug      540

auacugcuca ucgccccugc guacagcauu cggugcaucg gcguuuccaa ccgcgacuuu      600

guggagggca ugucaggggg aacuuggguc gauguggugu uagaacaugg uggcuguguc      660

accguuaugg cacaggacaa gccaacagug gauauugagc ugguaaccac gacugugagu      720

aauauggccg agguccgaag cuacugcuau gaggcuucua ucuccgacau ggccucagau      780

agcagauguc ccacacaagg ggaagcauac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuugguuga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcuaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguccacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg cgaagguaga gauuacacca     1080

aacucaccca gagccgaagc aacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cugguucaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcacacccc auuggaauaa caaagaggcu cuuguggaau uuaaggaugc ccacgcaaaa     1320

cggcagaccg ucgugguccu gggaucccag gagggcgccg ugcauaccgc uuuggcgggg     1380

gcacuagagg ccgaaaugga cggagcuaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag gggguuucau auaguuugug uacugccgca     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu agaaguccag     1560

uacgcuggca cagacggucc augcaaagug cccgcccaaa uggcaguuga caugcagacc     1620

cugacuccug uggggagguu aauuacagcg aauccaguca uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu gauuggagua     1740

ggcgagaaaa agaucacuca ccacuggcau cggucaggga guacaauagg aaaggcuuuu     1800

gaagccaccg ugcgcggugc aaaaagaaug gccguucucg gcgauacagc uugggacuuc     1860

gggagcgucg gaggcgcccu gaacucccuu gguaagggga uucaccagau cuucggcgca     1920

gcuuuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacucuc     1980

cugguguggc uuggacugaa uaccaagaac ggcuccaucu cauugacgug ucucgcccug     2040

gguggggugu uaauuuuccu gaguaccgcg gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4061
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 1 opt

<400>  4061
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggauccgca uacuauaugu accuggaccg gucugaugcc      120

ggugaagcga uuucauuccc caccacauug ggcguaaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcuacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacgucga uuguuggugc aauaccacaa gcaccugggu gguuuauggc      300

acuugucauc acaaaaaggg ugaagcccgc cgaucccgua gagcagugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagagucga aaacuggauc uuuaggaauc ccggguucgc ccuggcugca      480

gccgcuauug ccugguugcu cggaaguucc acaucucaga aagugaucua ucugguaaug      540

auacugcuca ucgcgccugc auacagcauu cggugcaucg gcguuuccaa ccgcgacuuu      600

guggagggca ugucaggggg aacuuggguc gauguggugu uagaacaugg uggcuguguc      660

accguuaugg cccaggacaa gccaacagug gauauugagc ugguaaccac gacugugagu      720

aauauggcug agguccgaag cuacugcuau gaagccucua ucuccgacau ggcaucagau      780

agcagauguc ccacacaagg ggaggcuuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuugguuga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgccaa auuugcgugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguccacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg ccaagguaga gauuacacca     1080

aacucaccca gagcagaagc uacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cugguucaua aggaaugguu ccacgauauc ccacucccgu ggcacgccgg ggcugacacu     1260

ggcacacccc auuggaauaa caaagaggcc cuuguggaau uuaaggaugc acacgccaaa     1320

cggcagaccg ucgugguccu gggaucccag gagggcgcug ugcauaccgc guuggcaggg     1380

gcccuagagg cugaaaugga cggagccaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag gggguuucau auaguuugug uacugcagcc     1500

uucacguuua caaagauccc ugcugagacc cuccauggaa cugugaccgu agaaguccag     1560

uacgccggca cagacggucc augcaaagug cccgcacaaa uggcgguuga caugcagacc     1620

cugacuccug uggggagguu aauuacagcu aauccaguca uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu gauuggagua     1740

ggcgauaaaa agaucacuca ccacugguau cggucaggga guacaauagg aaaggccuuu     1800

gaggcaaccg ugcgcggugc caaaagaaug gcuguucucg gcgacacagc cugggauuuc     1860

gggagcgucg gaggcgcacu gaacucccuu gguaagggga uucaucagau cuucggcgcu     1920

gccuuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacucuc     1980

cugguguggc uuggacugaa uaccaagaac ggcuccaucu cauugacgug ucucgcgcug     2040

gguggggugu uaauuuuccu gaguaccgcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4062
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 1 opt

<400>  4062
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggauccgca uacuauaugu accuggaccg gucugaugcc      120

gguaaggcga uuucauuccc caccacauug ggcguaaaca aaugcuauau acagaucaug      180

gacuuagggc acauguguga ugcuacuaug aguuacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacgucga uuguuggugc aauaccacaa gcaccugggu gguuuauggc      300

acuugucauc acaagaaagg ugaggcccgc cgaucccgua gagcagugac acuaccgucu      360

cauucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguacacc      420

aaacaccuua uuagagucga gaacuggauc uuuaggaauc ccggguucgc ccuggcugca      480

gccgcuauug ccugguugcu cggaaguucc acaucucaga aggugaucua ucugguaaug      540

auacugcuca ucgcgccugc auacagcauu cggugcaucg gcguuuccaa ccgcgacuuu      600

guggaaggca ugucaggggg aacuuggguc gauguggugu uagagcaugg uggcuguguc      660

accguuaugg cccaggacaa gccaacagug gauauugaac ugguaaccac gacugugagu      720

aauauggcug agguccgaag cuacugcuau gaggccucua ucuccgacau ggcaucagau      780

agcagauguc ccacacaagg ggaggcuuac cuugacaaac agagugacac ccaguaugug      840

ugcaagagga cuuugguuga ucggggaugg ggcaacgguu gugggcuguu cggaaaaggc      900

ucucuaguga caugcgccaa guuugcgugc uccaaaaaga ugaccggcaa gagcauacag      960

ccugaaaauc ucgaauaccg uauuaugcug ucgguccacg ggucucaaca cuccggaaug     1020

aucgugaacg acauugguca ugagacggau gaaaaucgcg ccaaaguaga gaucacccca     1080

aacucaccca gagcagaggc uacacuuggc ggguucggaa gucugggcuu ggacugugaa     1140

ccuaggacug gucucgauuu uagcgaccug uauuacuuaa ccaugaauaa caagcacugg     1200

cugguucaua aagagugguu ccacgauauc ccacucccgu ggcacgccgg ggcugacaca     1260

ggcacucccc auuggaauaa caaggaagcc cuuguggagu uuaaagaugc acacgccaag     1320

cggcagaccg ucgugguccu gggaucccag gaaggcgcug ugcauacagc guuggcaggg     1380

gcccuagagg cugagaugga cggagccaaa ggucgacugu cuagcggcca ccucaagugc     1440

cgccugaaga uggauaaacu uagacugaag gggguuucau auaguuugug uaccgcagcc     1500

uucacuuuua cgaaaauucc ugcugaaaca cuccauggaa ccgugacugu agagguccag     1560

uacgccggca ccgacggucc augcaaggug cccgcacaaa uggcgguuga caugcagaca     1620

cugaccccug uggggagguu aauaacugcu aauccaguca ucacagaauc cacggagaac     1680

agcaaaauga ugcuggaacu agaucccccg uucggcgacu cuuacauugu gaucggagua     1740

ggcgauaaga agauaaccca ccacuggcau cggucaggga guacuauugg aaaagccuuu     1800

gaggcaacag ugcgcggugc caagagaaug gcuguucucg gcgacaccgc cugggauuuc     1860

gggagcgucg gaggcgcacu gaacucccuu gguaaaggga uccaccagau cuucggcgcu     1920

gccuuuaagu cuuuguucgg aggcaugucg ugguuuagcc aaauucugau cgggacacuc     1980

cugguguggc uuggacugaa uacuaaaaac ggcuccauuu cauugaccug ucucgcgcug     2040

gguggggugu uaauauuccu gaguacggcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4063
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 1 opt

<400>  4063
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua ucaagguuga gaacuggauu uuuagaaauc cgggguucac acugguggca      480

gucgccaucg cuugguugcu cggaaguucc acuucucaga aggugauaua ccugguaaug      540

auccugcuca uugcccccgc guauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aaccuggguc gauguggugu uagagcacgg uggcuguguc      660

acaguuaugg cacaggacaa accuaccgug gauaucgaac ugguaacgac uacagugagu      720

aauauggccg agguccgcag cuacugcuac gaggcuucua uauccgacau ggccucagau      780

agccgauguc caacccaagg ggaagcauau cuugacaagc agagugacac ucaguacgug      840

ugcaaaagaa cauugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga ccugcgcuaa auuuacgugc uccaagaaga ugaccggcaa aagcauucag      960

cccgagaauc ucgaauaucg gaucaugcug ucgguccacg ggucucaaca uuccggaaug     1020

auugugaacg acacagguca cgagacugau gaaaaucgug ccaagguaga gguuaccccu     1080

aacucaccac gcgcggaggc cacacuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacug gucucgauuu uagcgaccug uacuauuuaa ccaugaauaa caaacauugg     1200

cuggugcaca aggagugguu ccacgauauc ccucucccau ggcaugcagg ggcugacaca     1260

ggcaccccgc acuggaauaa caaagaagcc cuugucgagu uuaaggaugc ucaugccaaa     1320

aggcagacug uggucgugcu gggaucccag gaaggcgcag uucacacggc cuuggcuggg     1380

gcgcuagagg cagagaugga cggagccaag ggucggcugu ucucuggcca ucucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag ggggugagcu acucauugug uacagcugcc     1500

uuuaccuuca cuaagguacc cgcagaaacc cuccacggaa cagucaccgu ggagguucag     1560

uaugccggca cugacggucc uugcaaagug ccagcucaaa uggccgucga caugcagaca     1620

cugacgcccg uggggagauu aaucaccgca aauccuguaa uuacugaaag uacagagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauagu gaucggaguu     1740

ggcgauaaga aaauuaccca ccauuggcac aggucugggu caacaaucgg aaaggcguuc     1800

gaggcuacug uccggggugc caaacgcaug gcagugcucg gcgacaccgc cugggauuuc     1860

gggagugugg gaggcgucuu uaacagccug gguaagggga uacaucagau uuucggcgcu     1920

gccuuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaaucuugau cgggacgcug     1980

cucguuuggc ugggacuuaa uaccaagaac ggcagcauuu cccugacaug uuuggcacuc     2040

ggugggguga ugaucuuccu gucaacugcu gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4064
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 1 opt

<400>  4064
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccaacuug ggcgugaaua aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacaaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aacacuacca gcacaugggu aguguacggc      300

accugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac ucuaccaucu      360

cacucgacaa ggaagcucca aacccgguca cagacguggc uggaaagccg cgaguauacu      420

aaacaucuua uuaaggugga gaauuggauc uuuagaaacc cgggguucgc acuggccgcu      480

guugccauag cgugguugcu cggaaguucc accucucaga aggugaucua ccuggucaug      540

auucugcuca ucgcacccgc cuauagcauu aggugcaucg gcguguccaa ucgggacuuu      600

guagaaggca ugucaggggg aacauggguu gauguggucu uagagcacgg uggcugugug      660

acugugaugg cucaggacaa accuaccguc gauauagaac ugguuacaac cacggugagu      720

aacauggccg agguacgcag cuacugcuac gaggcaucua uuuccgacau ggcuucagau      780

agccgauguc caacucaagg ggaagccuau cuugacaagc agagugacac acaguacgug      840

ugcaaaagaa ccuuggucga uaggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

ucucuaguga cuugcgcgaa auuuacaugc uccaagaaga ugaccggcaa aagcauccag      960

cccgagaacc ucgaauaucg gauuaugcug ucgguucacg ggucucaaca uuccggaaug     1020

aucgugaaug acaucgguca cgagacggau gaaaaccgug ccaaggucga ggugaccccu     1080

aauucaccac gcgcagaggc uacacuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacug gucucgauuu uagcgaccug uacuauuuaa ccaugaacaa uaaacauugg     1200

cugguacaca aggagugguu ccacgauauu ccucucccau ggcaugccgg ggcugacaca     1260

ggcacuccgc acuggaacaa uaaagaagcc cuuguugagu uuaaggaugc acaugccaaa     1320

aggcagaccg uggucgugcu gggaucccag gaaggcgcug uccacacagc guuggcaggg     1380

gcccuagagg cugagaugga cggagccaag ggucggcugu ucucuggcca ucucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag ggggugagcu acucauugug uaccgcagcc     1500

uuuacuuuca cgaagguucc cgcugaaaca cuccacggaa ccgugacugu agagguccag     1560

uaugccggca ccgacggucc uugcaaagug ccagcacaaa uggcgguuga caugcagaca     1620

cugacccccg uggggagauu aauaacugcu aacccuguca ucacagaaag uacggagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu gaucggagua     1740

ggcgauaaga aaauaaccca ccauuggcac aggucugggu caacuauugg aaaggccuuc     1800

gaggcaacag ugcggggugc caaacgcaug gcuguucucg gcgacaccgc cugggauuuc     1860

gggagugucg gaggcguguu uaauagccug gguaaggggg ugcaucagau cuucggcgca     1920

gcuuuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaaucuugau ugggacacug     1980

cucgucuggc ugggacuuaa cacuaagaau ggcagcaucu cccugaccug uuuggcccuc     2040

ggugggguua ugauuuuccu gucaacggcg gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4065
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 1 opt

<400>  4065
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgucaacg acaucgguca cgagaccgau gaaaaucgug cgaaggugga gguaacaccu     1080

aacucaccac gcgccgaggc aacucuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaaccg gucucgauuu uagcgaccug uacuauuuaa caaugaauaa caaacauugg     1200

cugguucaca aggagugguu ccacgauauu ccucucccau ggcaugcugg ggccgacacu     1260

ggcaccccgc acuggaauaa caaagaagcu cuuguggagu uuaaggaugc ccaugcaaaa     1320

aggcagacag ucgugguccu gggaucccag gaaggcgccg ugcacaccgc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag ggucggcugu ucucuggcca ucucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag gggguuagcu acucauugug uacugccgca     1500

uuuacguuca caaaggugcc cgccgaaacc cuccacggaa cuguaaccgu cgaggugcag     1560

uaugcuggca cagacggucc uugcaaaguu ccagcccaaa uggcagugga caugcagacc     1620

cugacucccg ucgggagauu aauaacagcg aauccuguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu aaucggagug     1740

ggcgauaaga aaauaacuca ccauuggcac aggucugggu caacaauugg aaaggcuuuc     1800

gaggccaccg uucggggugc aaaacgcaug gccguccucg gcgacacagc uugggauuuc     1860

gggagugugg gaggcguguu uaacagccug gguaagggga uccaucagau cuucggcgcc     1920

gcauuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaauuuugau cgggacucug     1980

cucgucuggc ugggacuuaa uaccaagaac ggcagcauuu cccugacgug uuuggcucuc     2040

ggugggguua ugauauuccu gucaaccgcc gugagugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4066
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 1 opt

<400>  4066
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccaacuug ggcgugaaua aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacaaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aacacuacca gcacaugggu aguguacggc      300

accugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac ucuaccaucu      360

cacucgacaa ggaagcucca aacccgguca cagacguggc uggaaagccg cgaguauacu      420

aaacaucuua uuaaggugga gaauuggauc uuuagaaacc cgggguucgc acuggccgcu      480

guugccauag cgugguugcu cggaaguucc accucucaga aggugaucua ccuggucaug      540

auucugcuca ucgcacccgc cuauagcauu aggugcaucg gcguguccaa ucgggacuuu      600

guagaaggca ugucaggggg aacauggguu gauguggucu uagagcacgg uggcugugug      660

acugugaugg cucaggacaa accuaccguc gauauagaac ugguuacaac cacggugagu      720

aacauggccg agguacgcag cuacugcuac gaggcaucua uuuccgacau ggcuucagau      780

agccgauguc caacucaagg ggaagccuau cuugacaagc agagugacac acaguacgug      840

ugcaaaagaa ccuuggucga uaggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

ucucuaguga cuugcgcgaa auuuacaugc uccaagaaga ugaccggcaa aagcauccag      960

cccgagaacc ucgaauaucg gauuaugcug ucgguucacg ggucucaaca uuccggaaug     1020

aucgugaaug acgagaaccg ugccaagguc gaagugacgc cuaauucacc acgcgcagag     1080

gcuacccuug guggcuucgg gagucuggga uuggauugug agcccagaac aggccucgac     1140

uuuagcgauc uguacuauuu aacuaugaac aauaaacacu ggcugguaca uaaggaaugg     1200

uuccacgaca ucccucuccc auggcacgcc ggugcugaua ccgggacacc gcauuggaac     1260

aauaaagagg cccuuguuga auuuaaggac gcacacgcca aaaggcagac uguggucgug     1320

cugggcuccc aggagggagc uguccauacc gcguuggcag gcgcccuaga agcugagaug     1380

gauggggcca agggacggcu guucucuggu caccucaagu gccgacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucauug uguacagcag ccuuuaccuu cacuaagguu     1500

cccgcugaga cgcuccaugg gacagugacc guagaagucc aguaugccgg aacugauggc     1560

ccuugcaaag ugccagcaca aauggcgguu gacaugcaga cccugacacc cguggguaga     1620

uuaauuaccg cuaacccugu cauaacugag aguacagaaa acuccaagau gaugcuggag     1680

cuagacccac ccuuugggga uagcuacauc gugauuggcg uaggagacaa gaaaaucacg     1740

caccacuggc auaggucugg cucaaccaua gggaaggccu ucgaagcaac ugugcgggga     1800

gccaaacgca uggcuguucu cggugauaca gccugggacu ucggcagugu cgggggagug     1860

uuuaauagcc ugggcaaggg uauucaccag aucuucgggg cagcuuuuaa aucccuuuuc     1920

ggcggaaugu cuugguuuuc gcaaaucuug auuggcaccc ugcucgugug gcuggggcuu     1980

aacacaaaga auggaagcau cucccugacu uguuuggccc ucggcggugu caugauuuuc     2040

cugucaaccg cgguuagugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4067
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 1 opt

<400>  4067
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua ucaagguuga gaacuggauu uuuagaaauc cgggguucgc acugguggcc      480

gucgcuaucg ccugguugcu cggaaguucc acaucucaga aggugauaua ccugguaaug      540

auccugcuca uugcgcccgc auauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aacuuggguc gauguggugu uagagcacgg uggcuguguc      660

accguuaugg cccaggacaa accuacagug gauaucgaac ugguaaccac gacugugagu      720

aauauggcug agguccgcag cuacugcuac gaggccucua uauccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacgug      840

ugcaaaagaa cuuugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauucag      960

cccgaaaauc ucgaauaucg gaucaugcug ucgguccacg ggucucaaca uuccggaaug     1020

auugugaacg acaccgguca cgagacagau gaaaaucgug cgaagguaga gguuacuccu     1080

aacucaccac gcgccgaggc aacccuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacag gucucgauuu uagcgaccug uacuauuuaa cuaugaauaa caaacauugg     1200

cuggugcaca aggagugguu ccacgauauc ccucucccau ggcaugcugg ggccgacacc     1260

ggcacaccgc acuggaauaa caaagaagcu cuugucgagu uuaaggaugc ccaugcaaaa     1320

aggcagaccg uggucgugcu gggaucccag gaaggcgccg uucacacugc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag ggucggcugu ucucuggcca ucucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag ggggugagcu acucauugug uacggccgca     1500

uuuacauuca ccaagguacc cgccgaaacu cuccacggaa ccgucacagu ggagguucag     1560

uaugcuggca ccgacggucc uugcaaagug ccagcccaaa uggcagucga caugcagacu     1620

cugacacccg uggggagauu aaucacggcg aauccuguaa uuaccgaaag uacugagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauagu gaucggaguu     1740

ggcgauaaga aaauuacaca ccauuggcac aggucugggu caaccaucgg aaaggcuuuc     1800

gaggccacag uccggggugc aaaacgcaug gccgugcucg gcgacacugc uugggauuuc     1860

gggagugugg gaggcgucuu uaacagccug gguaagggga uacaucagau uuucggcgcc     1920

gcauuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaaucuugau cgggacccug     1980

cucguuuggc ugggacuuaa uacgaagaac ggcagcauuu cccugaccug uuuggcucuc     2040

ggugggguga ugaucuuccu gucaacagcc gugagugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4068
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 1 opt

<400>  4068
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucaac acaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cauucgacca ggaagcucca gacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaccuua ucaagguuga gaacuggauu uuuagaaauc cgggguucgc acugguggcc      480

gucgcuaucg ccugguugcu cggaaguucc acaucucaga aggugauaua ccugguaaug      540

auccugcuca uugcgcccgc auauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aacuuggguc gauguggugu uagagcaugg uggcuguguc      660

accguuaugg cccaagacaa accuacagug gauaucgaac ugguaaccac gacugugagu      720

aauauggcug agguccgcag cuacugcuac gaggccucua uauccgacau ggcaucagau      780

agccgauguc caacacaggg ggaggcuuau cuugacaagc agagugacac ccaguacgug      840

ugcaaaagaa cuuugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauucaa      960

cccgaaaauc ucgaauaucg gaucaugcug ucgguccacg ggucucagca cuccggaaug     1020

auugugaacg acaucgguca ugagaccgau gaaaaucgug cgaagguaga gguuacaccu     1080

aacucaccac gcgccgaggc aacucuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaaccg gucucgauuu uagcgaccug uacuauuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggagugguu ccacgauauc ccucucccau ggcacgcugg ggccgacacu     1260

ggcaccccgc auuggaauaa caaagaagcu cuugucgagu uuaaggaugc ccacgcaaaa     1320

aggcagacag uggucgugcu gggaucccag gaaggcgccg uucauaccgc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag ggucggcugu ucucuggcca ccucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag ggggugagcu acucauugug uacugccgua     1500

ugcacggcag ccaagguccc cgcugaaaca cuccauggaa ccgugacugu ugaggugcaa     1560

uaugccggca ccgacggucc uuguaaaguc ccagcacaga uggcggugga caugcagaca     1620

cugacccccg uagggagauu aauuacugcu aauccuguga uaacagaaag uacggagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacaucgu uauuggaguc     1740

ggcgauaaga aaaucaccca ccacuggcau aggucugggu caacuauagg aaaggccuuc     1800

gaggcaacag ugcggggugc caaacgcaug gcugugcucg gcgacaccgc cugggauuuu     1860

gggagugucg gaggcguuuu caacagccug gguaagggga uucaccaaau cuucggcgca     1920

gcuuuuaaau cccuuuucgg aggcaugucu ugguuuucgc agaucuugau ugggacacug     1980

cucguguggc ugggacuuaa uacuaagaac ggcagcaucu cccugaccug cuuggcccuc     2040

ggugggguga ugauuuuccu gucaacggcg guaagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4069
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 1 opt

<400>  4069
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua ucaagguuga gaacuggauu uuuagaaauc cgggguucgc acugguggcc      480

gucgcuaucg ccugguugcu cggaaguucc acaucucaga aggugauaua ccugguaaug      540

auccugcuca uugcgcccgc auauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aacuuggguc gauguggugu uagagcacgg uggcuguguc      660

accguuaugg cccaggacaa accuacagug gauaucgaac ugguaaccac gacugugagu      720

aauauggcug agguccgcag cuacugcuac gaggccucua uauccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacgug      840

ugcaaaagaa cuuugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauucag      960

cccgaaaauc ucgaauaucg gaucaugcug ucgguccacg ggucucaaca uuccggaaug     1020

auugugaacg acgagaaucg ugcgaaggua gaaguuaccc cuaacucacc acgcgccgag     1080

gcaacacuug guggcuucgg gagucuggga uuggauugug agcccagaac uggccucgac     1140

uuuagcgauc uguacuauuu aaccaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaca ucccucuccc auggcacgcu ggugccgaua cagggacucc gcauuggaau     1260

aacaaagagg cucuugucga auuuaaggac gcccacgcaa aaaggcagac cguggucgug     1320

cugggcuccc aggagggagc cguucauaca gcuuuggcgg gcgcacuaga agccgagaug     1380

gauggggcua agggacggcu guucucuggu caccucaagu gccgacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucauug uguaccgccg cauuuacuuu cacgaaggua     1500

cccgccgaga cacuccaugg gaccgucacu guggaaguuc aguaugcugg aaccgauggc     1560

ccuugcaaag ugccagccca aauggcaguc gacaugcaga cacugacccc cguggguaga     1620

uuaaucacug cgaauccugu aauuacagag aguacggaaa acuccaagau gaugcuggag     1680

cuagacccac ccuuugggga uagcuacaua gugaucggcg uuggagacaa gaaaauuacc     1740

caccacuggc auaggucugg cucaacuauc gggaaggcuu ucgaagccac aguccgggga     1800

gcaaaacgca uggccgugcu cggugauacc gcuugggacu ucggcagugu cgggggagug     1860

uuuaacagcc ugggcaaggg uauacaccag auuuucgggg ccgcauuuaa aucccuuuuc     1920

ggcggaaugu cuugguuuuc gcaaaucuug aucggcacac ugcucguuug gcuggggcuu     1980

aauacuaaga acggaagcau uucccugacc uguuuggcuc ucggcggugu gaugaucuuc     2040

cugucaacgg ccgugagugc gugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4070
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 1 opt

<400>  4070
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugagacacgc cgaucccgua gaucugucuc gcuaagguau      360

cacuacaccc ggaagcucca aacgcgcuca cagacuuggc uggaaagcag agaguauaaa      420

aagcaucuua uuauggugga gaacuggauc uuuaggaauc caggguucgc cauaguuagu      480

guggcuauca ccuggcugau gggauccuug acaucucaga aggucauuua ccucgugaug      540

aucguacuga uuguuccggc auacagcauc uccugcauag gcgugucaaa ccgggaccug      600

gucgaaggca ugaguggggg aacuugggug gauguggucc ucgagcacgg uggcuguguu      660

accgaaaugg cccaggacaa acccacagug gauauugagu uaguaaccau gacggugagc      720

aauauggcug agguccgcuc uuauugcuac gaagccuccc ugucagacau ggcgagcgca      780

agucgauguc cuacucaagg ggagccaucu cuugauaagc aguccgacac acagagcgug      840

ugcaaaagaa ccuugggaga caggggcugg gguaacgggu guggaaucuu uggcaagggc      900

ucgcugguua cuugcucuaa auucacaugc uguaagaaga ugcccgggaa auccauucag      960

ccugaaaauc uagaguaucg gaucaugcuc ccagugcacg gaucacaaca uagugguaug     1020

aucgucaacg auauuggcca cgaaaccgac gagaaucgug ccaaggugga gguaacgccc     1080

aacagcccuc gcgcugaagc cacccuggga ggguuuggcu cccuuggucu ggauugcgag     1140

ccaagaacag gguuggacuu cucugaucuc uacuaucuga cuaugaauaa caaacauugg     1200

uuaguucaca aggaaugguu ucacgacaua ccgcugcccu ggcaugcagg cgcugauacc     1260

ggaacaccuc acuggaauaa caaagaggcc cucguggagu ucaaggacgc gcaugccaaa     1320

aggcagacug ucgugguccu uggcagccag gaaggggcag ugcacaccgc ucuggccgga     1380

gcuuuggagg ccgaaaugga uggugcaaag ggccggcuau uuucagggca ucugaagugu     1440

cgacucaaaa uggacaagcu gcgccuuaaa ggaguuaguu acucccugug cacagccgcu     1500

uucaccuuua cuaaggugcc agcggagacg uugcacggca caguaaccgu cgaagugcag     1560

agcgcaggua cugaugggcc cuguaaaguu ccugcccaaa uggcugugga caugcagacc     1620

cucacaccag ucggcagacu gaucaccgcc aaucccguga uuacugaguc uacagaaaac     1680

ucaaagauga uguuagagcu ggacccgccu uucggagaua guuauaucgu aauaggcgug     1740

ggggacaaga aaauuacgca ccauuggcac aggagcggau ccaccaucgg uaaggcauuu     1800

gaggccacug uucggggcgc uaaacgcaug gccguccuag gggauacagc augggacuuc     1860

ggaucugugg gcgguguguu caacucgcuc gggaagggca uccaucagau uuuuggagcg     1920

gcuuucaaaa gccuguuugg cgggaugucc ugguucucac aaauccuuau uggaaccuug     1980

cuggucuggc ucggccugaa uacaaagaac gguaguauau cucuuacuug ccuggcccuc     2040

gggggaguua ugaucuuuuu gagcaccgca guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4071
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 1 opt

<400>  4071
gggagaaagc uuaccaugag cgugggcauc gucgggcugc uccuuaccac agccauggcu       60

gcagagguua cuagaagggg auccgccuac uauauguacc uggaccggaa cgaugcgggu      120

gaagcuauuu cuuuccccac gaccuugggc augaauaagu gcuauauaca gaucauggac      180

uuagggcaca ugugugaugc cacaauguca uacgagugcc cuaugcugga cgaaggagug      240

gagccagaug acguagauug uuggugcaac acuaccagua cauggguggu cuauggcacc      300

ugucaucaca aaaaggguga agcacgccga agccguagag ccgugacucu accgucccau      360

ucuacaagga aacuccaaac ccggucgcag acguggcugg agucacgcga guacacuaag      420

caccuuauua gaguugaaaa uuggaucuuu aggaaccccg gguucgcucu ggcagccgcu      480

gccauugcgu gguugcucgg aagcaguacc ucccagaaag ugaucuaucu ggucaugaua      540

cugcucaucg ccccugcaua cucuauucgg ugcaucggcg ugagcaaucg cgacuuugua      600

gagggcaugu ccgggggaac auggguugau guggucuuag aacauggugg cugugugacu      660

gugauggcuc aggacaagcc aaccgucgau auugagcugg uuacaaccac ggugucaaac      720

auggccgagg uacgaaguua cugcuaugaa gcaagcaucu cugacauggc uuccgauuca      780

agauguccca cucaagggga ggccuaccuu gacaagcaga gcgacacaca guaugugugc      840

aaaaggaccu uggucgaucg gggauggggc aaugguugug ggcuguucgg aaagggcagu      900

cuagugacuu gcgcgaaauu ugccugcucu aagaaaauga caggcaaguc cauacagccu      960

gaaaaccucg aguaccguau uaugcugagc guucacgggu cgcaacacuc uggaaugauc     1020

gugaaugaca ccggucauga aacggaugag aaccgcgcaa aggucgagau uaccccaaau     1080

ucccccagag cugaagccac acuuggcggg uucggaucac ugggcuugga cugugagccu     1140

aggacugguc ucgauuuuag ugaccuguau uacuuaacca ugaacaauaa acacuggcug     1200

gugcauaagg aaugguucca cgauauccca cucccguggc acgcuggggc cgacacaggc     1260

acuccccauu ggaacaauaa agaggcacuu guagaauuua aggaugccca cgcuaaacgg     1320

cagaccguug ugguccuggg aagccaggag ggcgcggugc auacagcauu ggccggggcu     1380

cuagaggccg aaauggacgg agcaaagggu cgacuguccu cuggccaccu caaaugccgc     1440

cugaagaugg auaaacuuag acugaagggg gucagcuauu cauuguguac cgccgcuuuc     1500

acuuuuacga agaucccugc cgagacacuc cauggaaccg ugacuguuga agugcaguac     1560

gcaggcaccg acgguccaug caaaguaccc gcgcaaaugg cugucgacau gcagacacug     1620

accccugugg ggagguuaau uacugccaac ccaguuauaa cagagaguac ggaaaacucc     1680

aagaugaugc uggagcuaga ucccccguuc ggcgacagcu acaucgugau uggagucggc     1740

gagaaaaaga ucacccacca cuggcaucgg ucugggucaa cuauaggaaa ggcauuugaa     1800

gccacagugc gcggugcuaa aagaauggcc guacucggcg auaccgcaug ggacuucggg     1860

agugugggag gcgcucugaa uagccuuggu aaggggauuc accagaucuu cggcgccgcg     1920

uuuaaauccu uguucggagg caugucuugg uuuucgcaaa uccugauugg gacacuccug     1980

auguggcuug gacugaacac uaagaauggc agcaucuccu ugaugugucu cgcccugggu     2040

gggguuuuaa uuuuccuguc aaccgcuguc agugcaugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  4072
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 1 opt

<400>  4072
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uagaaggaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaaaggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaaaaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4073
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 1 opt

<400>  4073
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggaug uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccaucg cgugguugcu cggaagcagu acaucccaga aagugauuua ucuggucaug      540

auccugcuca uagcacccgc cuacucuauc cggugcauug gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauaucgagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca uuucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauccag      960

ccugaaaacc ucgaguaccg uauaaugcug agcguucacg ggucgcaaca cucuggaaug     1020

auugugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauu ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aaucacagcc aacccaguua uuacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauagu gaucggaguc     1740

ggcgagaaaa agauuacuca ccacuggcau cggucugggu caacaaucgg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uacaccagau uuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

ggugggguuu uaaucuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4074
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 1 opt

<400>  4074
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaugacca guacaugggu ggucuauggc      300

accugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ucuaccgucc      360

cauucuacaa ggaaacucca aacccggucg cagacguggc uggagucacg cgaguacacu      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu accucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacauggguu gauguggucu uagaacaugg uggcugugug      660

acugugaugg cucaggacaa gccaaccguc gauauugagc ugguuacaac cacgguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacucaagg ggaggccuac cuugacaagc agagcgacac acaguaugug      840

ugcaaaagga ccuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga cuugcgcgaa auuugccugc ucuaagaaaa ugacaggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacggau gagaaccgcg caaaggucga gauuacccca     1080

aauuccccca gagcugaagc cacacuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacug gucucgauuu uagugaccug uauuacuuaa ccaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacaca     1260

ggcacucccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacagc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uaccgccgcu     1500

uucacuuuua cgaagauccc ugccgagaca cuccauggaa ccgugacugu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagaca     1620

cugaccccug uggggagguu aauuacugcc aacccaguua uaacagagag uacggaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggucugggu caacuauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauaccgc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4075
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 1 opt

<400>  4075
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgcuaugg cccaggacaa gccaacagug gauauugagc uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaagcuagca ucucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaggcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

cuuguuaaug acacggguca ugaaaccgau gagaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaagc uacucugggc ggguucggau cauugggccu cgacugugag     1140

ccuaggaccg gucuggauuu uagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgcccaua aggaaugguu ccacgauauu ccacuuccgu ggcacgcagg ggccgcuacu     1260

ggcaccccuc auuggaacaa uaaagaggcg cuggucgaau uuaaggacgc acacgccaaa     1320

cggcagacag ugguaguuuu gggaagccag gagggcgcug ugcauaccgc ccuagcaggg     1380

gcccuggagg cugaaaugga uggagccaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggacaagcu gagauugaaa ggggucagcu auucacucug uacugcagcg     1500

uucacguuua caaagauccc ugcugagacc guggauggaa cugucaccgu ggaaggccag     1560

uacgguggga cagacggccc augcaaaguu cccgcccaaa uggcagugga caugcagacc     1620

cugacuccug uaggaagguu aaucacagcc aacccaguca uuacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauagu gaucgggguu     1740

ggagagaaaa agauuacuca ucacuggcac cggucugguu caacaaucgg caaggcuuuu     1800

gaagccaccg ugcgcggggc aaaaagaaug gcuguccucg gagauacagc cugggacuuc     1860

ggcagugugg guggggcgcu gaauagccuu ggcaagggaa uacaucagau uaucggcgcc     1920

gcuuucaaau ccuuguuugg gggaaugucu ugguucucgc aaauccugau uggcacucuc     1980

cugauguggc uuggucugaa caccaagaau gggagcaucu ccuugaugug ucucgcacug     2040

ggaggcguau uaauuuuucu gucaacggcc gugagugguu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4076
<211>  2275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 1 opt

<400>  4076
gggagaaagc uuaccauggg cgccgacgcu agcaucggga uugugucccu gcuccuuacc       60

acagucaugg cagccgagau aacuagaagg ggaucugcgu acuauaugua ccuggaucgg      120

ucagacgcug guaaggccau caguuucguu acgaccuugg gcgugaacaa augccacgua      180

cagauuaugg auuuagggca uaugugugac gcaacaauga gcuaugaaug ccccaugcug      240

gaugagggag uggaaccuga cgaugucgac uguuggugca auacuaccuc cacaugggug      300

guuuacggca ccugucacca uaagaaaggu gaggcccgcc gaucucguag agcugugacu      360

cuaccaucgc acucaacaag gaagcuccaa acccggagcc agacguggcu ggaaagucgc      420

gaguauacua aacaucuuau caaggucgag aacuggauuu uuagaaaucc gggguucgca      480

cugguggccg uagcuaucgc cugguugcuc ggauccucua ccagccagaa gguuauauac      540

cuggugauga uccugcucau ugcgcccgca uauuccauca ggugcauugg cgucucaaac      600

cgggauuuug uggaaggcau gaguggggga acaugggugg acgucguuuu agagcacggu      660

ggcuguguga cuguaauggc ccaggauaaa ccuaccgugg acaucgaacu ggucacaacc      720

acggugagca auauggcuga gguucgcucu uacugcuacg aggccuccau aucagauaug      780

gcaagcgaca gucgaugucc aacucaaggg gaggcuuauc uugacaagca gucugauaca      840

caguacgugu gcaaaagaac cuuggucgac aggggauggg gcaacgguug ugggcuguuc      900

ggaaagggcu cccuagugac uugcgccaaa uuuacaugca gcaagaagau gaccggcaaa      960

ucgauucagc ccgaaaaucu cgaauaucgg aucaugcugu cuguacacgg gucccaacau     1020

ucaggaauga uuguuaacga ugagaaucgu gcgaaggugg aagucacgcc uaacagucca     1080

cgcgccgagg caacccuugg uggcuucggg agccugggau uggacuguga gcccagaaca     1140

ggccucgauu uuuccgaccu guacuauuua acuaugaaua acaaacacug gcuggugcau     1200

aaggaauggu uccacgauau cccucuccca uggcacucug gugcugacac cgagacaccg     1260

cauuggaaua acaaagaagc ccuugucgag uuuaaggaug cucacgccaa aaggcagacu     1320

gugguugugc uggggagcca ggaaggcgca guacauaccg ccuuggcugg agcgcuagag     1380

gcagagaugg acggcgccaa ggggcggcug ucaaguggac accucaagug ccgacugaaa     1440

auggauaaac uucgccugaa gggugucucc uacagcuugu guacagcugc cuucaccuuu     1500

acuaaggugc ccgcagaaac gcuccauggc acaguuaccg uggaggucca guaugccggg     1560

agagacggac cuugcaaagu gccagcucaa auggccguag acaugcagac ucugaccccc     1620

gugggcaggu uaaucacagc aaauccuguu auuaccgaau cuacugagaa cucaaagaug     1680

augcuggaac uagauccacc cuucggugac aguuacauag ucaucggggu gggcgauaag     1740

aaaauuacac accacuggca ucggagcgga uccaucauag gcaaggcguu ugaggcuacg     1800

gugcgcgggg ccaaaagaau ggcaguccuc ggagacaccg ccugggauuu cgguucuguu     1860

ggcggggugu ucaacucgcu gggaaagggc auucaccaga ucuuuggugc ugccuucaaa     1920

agccuuuuug ggggcauguc cugguucuca caaaucuuga uuggaacucu gcucgugugg     1980

cugggccuua auacaaagaa cgggaguauc ucucugaccu guuuggcacu cggaggcgua     2040

augauuuuuc ugagcacagc ugucuccgcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2220

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2275


<210>  4077
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 1 opt

<400>  4077
gggagaaagc uuaccauggg cgccgacacc agcaucggga uuauaggacu gcuccuuaca       60

acugcuaugg cagccgagau cacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

ucugacgcug gcaaggccau uucauucgca accacaaugg gggugaacaa augccacguc      180

cagaucaugg auuugggaca uaugugugac gccacuauga guuaugaaug ccccauguua      240

gaugagggcg uugaaccuga cgauguggac uguuggugca auaccacaag caccugggua      300

guguacggua cuugucacca uaagaaaggg gaggcucgcc caucccgacg ugcagucaca      360

cugccgucuc acucgaccag aaagcuacaa acgaggucac agacuuggcu cgaaagccgg      420

gaguauacca aacaucugau uaagguggag aacuggaucc uucgcaaucc cggauuuaca      480

guugccgcuc uggccauggc gugguugcuc ggcaacagua cuucccagag agugauauac      540

cuggucauga uccugcucau ugcacccgcc uauucuauca ggugcauugg cgugagcaau      600

cgggauuucg uagaagggau guccggaggu accuggguug acguggucuu agagcacggc      660

ggguguguga cagugauggc ucaggauaag ccaaccgucg acaucgaacu gguuacgacu      720

acagugucaa acauggccga gguacgcagu uacugcuacg aggcaagcau aucugauaug      780

gcuuccgacu cacgaugucc cacccaagga gaagccuauc uugacaaaca gagcgauacu      840

caguacgugu gcaagagaac auuggucgac aggggcuggg guaaugggug uggacuguuu      900

ggcaaaggca gucuagugac cugcgcgaag uucacgugcu cuaaaaagau gaccgggaag      960

uccauucagc cugagaaccu cgaauaucgg aucaugcuga gcguucacgg aucgcaacau     1020

ucugguauga uugugaauga uaucggccac gagacagacg aaaaccgugc caaagucgag     1080

gugacuccaa auuccccucg cgcagaggcu acccuugggg gauuuggcuc acuggguuug     1140

gauugugaac cuagaacagg gcucgacuuc agugaucugu acuauuuaac uaugaacaau     1200

aagcauuggc ugguacacaa agagugguuu cacgacaucc cacucccgug gcaugccggc     1260

gcugauaccg gaacacccca cuggaacaau aaggaagccc uuguugaguu caaagacgca     1320

caugccaaga ggcagaccgu ggucgugcug ggcagccagg aaggggcugu ccacacugcg     1380

uuggcaggag cccuagaggc ugagauggau ggugccaaag gccggcuguu uuccgggcau     1440

cucaagugcc gaauuaagau ggacaaacug cgccuuaagg gagugucuua cagccugugu     1500

acggcagccu ucacauuuuc aaaaguuccu gcugaaaccu ugcacggcac ugugaccgua     1560

gagguccagu augccgguag agacgggcca ugcaaggugc ccgcacaaau ggcgguugau     1620

augcagacac ucaccccugu gggcaggcug auaacugcua acccaaucau uacagaaagu     1680

acggagaacu cccggaugau guuagaacug gaucccccgu ucggagacag cuacaucguc     1740

auaggcgugg gggacaaaaa gauuacccac cauuggcacc gcucuggauc aacuaucggu     1800

aaggccuuug aggcaacagu aagaggcgcc aaaaggaugg cugugcuagg ggauaccgcc     1860

ugggacuucg gaaguguugg cggugucuuc aauagccucg ggaagggcgu gcaucaggug     1920

uuuggagcag cuuucaaauc ccuguuuggc gggaugucuu gguucucgca aauccuuauu     1980

ggaacauugc uggucuggcu cggccugaac acuaagaaug guagcaucuc ccuugccugu     2040

cuggcguugg gaggggccau gauuuuucuc ucaaccgcug uuagugcaug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4078
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 1 opt

<400>  4078
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagauau cgugcuggaa cacggcgggu gugucacagu gauggcacaa      180

gacaaaccua cuguugauau ugaguuggug acgaccacag ucaguaauau ggccgaagug      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag guggaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ccauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ugaguucaag gaugcacacg ccaagagaca gacaguggua      840

guguugggcu cccaggaagg ggcuguccau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auccccgcug agacccugca cggcacuguu acaguggaag uccaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacguuaac cccgguuggc     1140

cgacugauaa cagcaaaucc cgugauuacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgucauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4079
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 1 opt

<400>  4079
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4080
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 1 opt

<400>  4080
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgg cuaugaaacc      540

gaugaggaca gggccaaggu ggaagucacg cccaacaguc cucgggcuga ggcaacucug      600

ggaggguuug gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau      660

cuguacuacc ucacaaugaa uaacaaacau ugguuaguuc acaaggaaug guuucaugac      720

auuccccugc cuuggcacgc cggagcugac acuggcaccc cacacuggaa uaacaaagag      780

gcccuugugg aguucaagga ugcgcaugca aagagacaga caguaguggu cuugggcucc      840

caggaagggg ccgugcacac cgcucuggcc ggagcacuag aagcugagau ggacggugcc      900

aaaggcaggc ucuuuucagg gcaucugaag ugucggcuua aaauggauaa gcugcgcuug      960

aaaggaguua gcuauagucu cugcacggcg gccuucacuu uuacaaaggu gcccgcagag     1020

acccugcacg gcacugucac aguggaagua caauacgcug guaccgacgg gccuuguaaa     1080

aucccaguuc agauggccgu ggauaugcag acguuaaccc cggucggccg acugauuaca     1140

gcuaaucccg ugaucacuga gucuaccgaa aacuccaaga ugaugcucga gcuugacccu     1200

ccauucggag auagcuauau agucauuggc gugggggaca agaaaaucac acaccauugg     1260

cacagaucgg gaucuacuau ugguaaggcc uuugaagcaa ccguuagggg cgccaaacgg     1320

auggcugugc ugggggauac agcgugggac uucggauccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4081
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 1 opt

<400>  4081
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4082
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 1 opt

<400>  4082
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauacc      540

ggcuaugaaa cggacgagaa uagggccaag gucgaaguua cucccaacag uccucgggcu      600

gaggcaaccc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacagggcug      660

gacuucucug aucuguacua ccucacuaug aauaacaaac auugguuagu gcacaaggaa      720

ugguuucaug acauuccccu gccuuggcac gccggagcug acaccggcac accacacugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcaug caaagagaca gaccgugguc      840

guguugggcu cccaggaagg ggccguucac acggcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcaa gcucuuuuca gggcaucuga aauguaggcu uaagauggau      960

aaacugcggu ugaagggagu gagcuauagu cucugcacug cggccuucac auuuaccaaa     1020

guccccgcag agacucugca cggcacagug accguagaag uucaauacgc ugguacggac     1080

gggccuugua agaucccagu gcagauggcc gucgauaugc agaccuuaac accggugggc     1140

cgccugauua cugcuaaucc cgucaucacc gagucuacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuau auagugauug gcguugggga caaaaagauc     1260

acucaccauu ggcaccgauc gggaucuacc auugguaaag ccuuugaagc aacagugaga     1320

ggcgccaaga ggauggcugu acugggggau accgcguggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4083
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 1 opt

<400>  4083
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaccgu gaacgauauc      540

ggcuaugaaa cggacgagaa uagggccaag gucgaaguua cucccaacag uccucgggcu      600

gaggcaaccc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacagggcug      660

gacuucucug aucuguacua ccucacuaug aauaacaaac auugguuagu gcacaaggaa      720

ugguuucaug acauuccccu gccuuggcac gccggagcug acaccggcac accacacugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcaug caaagagaca gaccgugguc      840

guguugggcu cccaggaagg ggccguucac acggcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcaa gcucuuuuca gggcaucuga aauguaggcu uaagauggau      960

aaacugcggu ugaagggagu gagcuauagu cucugcacug cggccuucac auuuaccaaa     1020

guccccgcag agacucugca cggcacagug accguagaag uucaauacgc ugguacggac     1080

gggccuugua agaucccagu gcagauggcc gucgauaugc agaccuuaac accggugggc     1140

cgccugauua cugcuaaucc cgucaucacc gagucuacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuau auagugauug gcguugggga caaaaagauc     1260

acucaccauu ggcaccgauc gggaucuacc auugguaaag ccuuugaagc aacagugaga     1320

ggcgccaaga ggauggcugu acugggggau accgcguggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4084
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 1 opt

<400>  4084
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgaugaa      540

aauagggcca aggucgaggu uacccccaac aguccucggg cugaagcaac gcugggcggg      600

uuuggcagcu ugggacucga cugcgagcca cgcacugguc uggauuucuc ugaccuguau      660

uaccucacca ugaauaacaa acauugguua gugcacaagg agugguuuca ugauauuccc      720

cugccuuggc acgccggggc ugacacagga acuccacacu ggaauaacaa agaagcccuu      780

guagaguuca aggacgcgca ugcaaagaga cagaccgugg ucguguuggg cucccaggaa      840

ggcgccguuc acacagcucu ggccggggca cuagaggcug aaauggaugg agccaaaggu      900

aggcucuuuu caggccaucu gaagugucgg cuuaaaaugg acaagcugcg cuugaaaggg      960

gugagcuaca gucucugcac cgcggccuuc acguuuacua agguccccgc agagacacug     1020

cacggaaccg ugacuguaga gguucaauau gcuggcacag augguccuug uaaagugcca     1080

gcccagaugg cugucgacau gcagaccuua acgccggugg ggcgacugau caccgccaau     1140

cccgucauua cagaaucuac ugagaacucc aagaugaugc ucgaacuuga uccuccauuc     1200

ggcgacagcu acaucgugau aggaguuggc gauaagaaaa uuacccacca uuggcacaga     1260

ucggggucua caaucggaaa ggcauuugag gccacuguga ggggugcuaa acggauggcg     1320

guacugggcg acaccgcaug ggauuucggg uccugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  4085
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 1 opt

<400>  4085
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgg cuaugaaacc      540

gaugagaaca gggccaaggu ggaagucacg cccaauaguc cucgggcuga ggcaacucug      600

ggaggguuug gcagcuuggg ucucgacugc gagccacgca ccgggcugga uuucucugac      660

cuguacuacc ucacaaugaa caauaaacau ugguuaguuc acaaggaaug guuucaugau      720

auuccccugc cuuggcacgc cggagcugac acuggcaccc cacacuggaa caauaaagag      780

gcccuugugg aguucaagga cgcgcaugca aagagacaga caguaguggu cuugggcucc      840

caggaagggg ccgugcacac cgcucuggcc ggagcacuag aagcugagau ggauggugcc      900

aaaggcaagc ucuuuucagg gcaucugaaa uguaggcuua agauggacaa acugcgguug      960

aagggaguua gcuauagucu cugcacggcg gccuucacuu uuacaaaagu gcccgcagag     1020

acccugcacg gcacugucac aguggaagua caauacgcug guaccgaugg gccuuguaag     1080

aucccaguuc agauggccgu ggacaugcag acguuaaccc cggucggccg ccugauuaca     1140

gcuaaccccg ugaucacuga gucuaccgaa aauuccaaga ugaugcucga gcuugauccu     1200

ccauucggag acagcuauau agucauuggc gugggggaua aaaagaucac acaccauugg     1260

caccgaucgg gaucuacuau ugguaaagcc uuugaagcaa ccguuagagg cgccaagagg     1320

auggcugugc ugggggacac agcgugggau uucggauccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4086
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 1 opt

<400>  4086
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu cccugagaug caucggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu ggucuuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau ugaguuaguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuaccugga caagcagagc gauacccagu auguuugcaa acgaacauug      360

guggaccgug gcugggguaa cgggugugga cucuuuggca agggcagucu ggugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uucaaccgga gaaucuugag      480

uacagaauca ugcugagcgu ccauggaucc cagcacucag guaugauagu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauca cgcccaacag uccucgggca      600

gaggccacuc uagggggauu uggcagccuc ggucuggauu gcgagccacg caccgggcug      660

gacuucucug aucucuauua cuuaacaaug aauaacaaac acuggcuggu ucauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcguuggu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

guccugggcu cccaggaagg ggcugugcau accgcccuag caggagcucu cgaagccgag      900

auggacggug cgaaaggcag gcugucaagc gggcaccuua agugucggcu gaaaauggau      960

aaguugcgcc ucaaaggagu uaguuacucu cugugcacgg ccgcauuuac uuucacaaag     1020

auccccgcug agaccuuaca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacgcugac cccggugggc     1140

cgacucauua cagcaaaucc cgucaucacu gaguccaccg aaaacagcaa gaugaugcuu     1200

gagcuggacc cuccauuugg agauucguac auagugauug gcgucgggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu auugguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu uuugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4087
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 1 opt

<400>  4087
gggagaaagc uuaccaugag caagaaagug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacag      180

gacaagccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

caaggagaag ccuacuuaga caaacagagc gauacccagu auguuugcaa gcgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca aaggcagucu cgugaccugc      420

gcaaaguucg ccuguuccaa gaaaaugacu gggaagucua uccagccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc caacacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaaa gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaagc acugguuagu ucauaaagaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaagg aggcgcuugu ggaguucaag gaugcacacg ccaaaagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaagggcag gcucucaagc gggcaccuga aaugucggcu uaagauggau      960

aaacugcgcu ugaagggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaaa     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaguaugc cgguaccgac     1080

gggccuugua agguaccagc ucaaauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga gaaaaagauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaaag ccuucgaagc uaccgugagg     1320

ggcgcgaagc ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4088
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 1 opt

<400>  4088
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu gguauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4089
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 1 opt

<400>  4089
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcaa gcucucaagc gggcaccuga aauguaggcu uaagauggau      960

aaacugcggu ugaagggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaaa     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua agguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgccugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga gaaaaagauu     1260

acacaucacu ggcaucgauc uggauccacu aucgguaaag ccuucgaagc uaccgugaga     1320

ggcgcgaaga ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4090
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 1 opt

<400>  4090
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauacgc agaccuuaac accggugggc     1140

cgacugauca cugcaaaucc cgucauaacc gaguccacag aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acucaucacu ggcauagauc uggauccacc aucgguaagg ccuucgaggc uacagugagg     1320

ggcgcgaaac ggauggcagu ucugggggac accgccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4091
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 1 opt

<400>  4091
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaaauu      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag guggaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ccauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ugaguucaag gaugcacacg ccaagagaca gacaguggua      840

guguugggcu cccaggaagg ggcuguccau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auccccgcug agacccugca cggcacuguu acaguggaag uccaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacguuaac cccgguuggc     1140

cgacugauaa cagcaaaucc cgugauuacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgucauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4092
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 1 opt

<400>  4092
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgcggc guauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4093
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 1 opt

<400>  4093
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacaugguuu cggauucacg cuguccaacu      300

cagggagagg cuuacuuaga caagcagagc gauacccagu augugugcaa acgaacacug      360

gucgaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gccaaauucg cauguuccaa gaagaugacu gggaaaucua uccaaccgga aaaucuggag      480

uacagaauaa ugcuuagcgu gcauggaucc cagcacucag guaugaucgu caacgauaca      540

ggccaugaaa ccgacgagaa uagggccaag guugaaauua cgcccaacag uccucgggcu      600

gaggcaacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu gcauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcug acacuggcac cccacauugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcacg caaagagaca gacagugguc      840

guguugggcu cccaggaagg ggccguucau accgcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg cggccuuuac uuucacaaag     1020

auucccgcag agacccugca cggcacuguc acaguggaag uacaauaugc ugguaccgac     1080

gggccuugua aaguuccagc ccagauggcu guggauaugc agacguuaac cccggucggc     1140

cgacugauca cagccaaucc cgugauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg cauucgaggc caccguuagg     1320

ggcgcuaaac ggauggcggu gcugggggac acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4094
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 1 opt

<400>  4094
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccauug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgugauug gcgucgggga aaagaaaauc     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg ccuucgaggc uacugugagg     1320

ggcgcgaaac ggauggcagu ucugggggac accgccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4095
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 1 opt

<400>  4095
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcauugggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4096
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 1 opt

<400>  4096
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cuggcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu ggucuuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccca cuguggauau cgaguuaguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccuacu      300

cagggagaag ccuaccugga caagcagagc gauacccagu auguuugcaa acgaacauug      360

guggaccgug gcugggguaa cgggugugga cucuuuggca agggcagucu ggugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccaga gaaucuugag      480

uacagaauaa ugcugagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgccgaacag uccccgggca      600

gaggccacuc uagggggauu uggcagccuc ggucuggauu gcgagccucg caccgggcug      660

gacuucucug aucucuauua cuuaacaaug aauaacaaac acuggcuggu ucauaaggaa      720

ugguuucacg acaucccacu ucccuggcac gcuggagccg acacuggcac cccucauugg      780

aauaacaaag aggcguuggu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

guccugggcu cccaggaagg ggcugugcau accgcccuag caggagcucu cgaagccgag      900

auggacggug cgaaaggcag gcugucaagc gggcaccuua agugucggcu gaaaauggau      960

aaguugcgcc ucaaaggagu uaguuacucu cugugcacgg ccgcauuuac uuucacaaag     1020

auuccagcug agaccuuaca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggcccugua aaguaccugc ucagauggcc guugauaugc agacgcugac cccagugggc     1140

cgacucauca cagcaaaucc ggucauaacu gaguccaccg aaaacagcaa gaugaugcuu     1200

gagcuggauc ccccuuuugg agacucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu uuugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4097
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 1 opt

<400>  4097
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggca cccaggaagg ggcugugcau acggcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucuccuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uagcuacagu cucugcacug ccgcauuuac auucaccaag     1020

auucccgcug agacucugca cggcacagug accgucgaag ugcaauaugc cgguacggac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agaccuuaac accggugggc     1140

cgacugauca cugcaaaucc cgucauaacc gagucuacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauagcuac auugugaucg gcgucgggga aaagaaaauu     1260

acucaucacu ggcauagauc gggaucuacc aucgguaagg ccuucgaggc uacagugagg     1320

ggcgcgaaac ggauggcagu ucugggggac accgccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4098
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 1 opt

<400>  4098
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguauuagc ucagauggcc guugauaugc agacgcugac cccagugggc     1140

cgacucauca cagcaaaucc ggucauaacu gaguccaccg aaaacagcaa gaugaugcuu     1200

gagcuggauc ccccuuuugg agacucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu uuugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4099
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 1 opt

<400>  4099
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaagcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaggccgag      900

auggauggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggac      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug aaacccugca cggcacugug acagucgagg ugcaauaugc cgguaccgau     1080

gggccuugua aaguaccagc ucagauggcc guugacaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaauccaccg agaacagcaa gaugaugcuc     1200

gaacuugauc cuccauuugg agacucguac auugugaucg gcgucgggga gaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggau acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4100
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 1 opt

<400>  4100
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccgcuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacugggcug      660

gacuucucug aucuguauua ccucaccaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcgg acacaggcac uccacauugg      780

aauaacaaag aggcacuugu ggaguucaag gaugcccacg cuaagagaca gaccguagug      840

gucuugggcu cccaggaagg ggccgugcau acagcacugg cuggagcccu agaagcggag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcaccg cagcuuuuac guucacuaag     1020

auucccgccg agacacugca cggcaccgug acugucgaag ugcaauaugc ugguacagac     1080

gggccuugua aaguaccagc ccagauggca guugauaugc agaccuuaac gccggugggc     1140

cgacugauca ccgccaaucc cgucauaaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg cuuucgaggc gacugugagg     1320

ggcgcaaaac ggauggccgu ucugggggac accgcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4101
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 1 opt

<400>  4101
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc aguacgaccg ugagcaauau ggccgaagua      240

cgguccuacu gcuaugaggc gacaauuucu gacaucgcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacagcugua      840

guguugggcu cccaggaagg ggccguccau accgcacugg cuggagcccu agaagcggag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg cagcuuuuac uuucacaaag     1020

auccccgccg agacccugca cggcacuguu acaguggaag uccaauaugc ugguaccgac     1080

gggccuugua aagugccagc ccagauggca guagauaugc agacguuaac cccgguuggc     1140

cgacugauaa cagccaaucc cgugauuacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgucauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg cuuucgaggc gaccgucagg     1320

ggcgcaaaac ggauggccgu gcugggggac acagcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4102
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 1 opt

<400>  4102
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucuccaag     1020

auucccgcug agacacugca cggcaccgug acugaagagg uccaauaugc cgguacagac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agaccuuaac gccgguuggc     1140

cgacugauca ccgcaaaucc cgugauaaca gaaagcacug agaacucgaa gaugaugcuc     1200

gaacuugacc cuccauuugg agauucuuac auugucaucg gcguggggga gaagaaaauu     1260

acccaucacu ggcauagauc cggaucaaca aucgguaagg ccuucgaggc uacugucagg     1320

ggcgcgaaac ggauggcagu gcugggggac accgccuggg auuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4103
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 1 opt

<400>  4103
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaau uacaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacgcugac cccgguuggc     1140

cgacucauca cagcaaaucc cgugauaacu gaguccaccg aaaacagcaa gaugaugcuu     1200

gagcuggacc cuccauuugg agauucguac auugucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu guugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4104
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 1 opt

<400>  4104
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcauc ucuaacaggg acuucguuga ggggauguca      120

ggagguaccu ggguggaugu agugcuggaa cacggcgggu gugucacagu gauggcacaa      180

gacaaaccua cuguugauau ugaguuggug acgaccacag ucaguaauau ggccgaagug      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag guggaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcauuuug ggucucgauu gcgagccacg caccgggcug      660

gacuucagcg aucuguauua ccucacaaug aauaacaaac acugguuagu ccauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ugaguucaag gaugcacacg ccaagagaca gacaguggua      840

guguugggcu cucaggaagg ggcuguccau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucuccuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gagcuacagu cucugcacgg ccgcauuuac uuucacaaag     1020

auacccgcug agacccugca cggcacuguu acaguggaag uccaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacguuaac cccgguuggc     1140

cgacugauua cagcaaaucc cgugaucacu gagucuaccg aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauagcuac auugucaucg gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc gggaucuacu auugguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4105
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 1 opt

<400>  4105
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug uuaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg cggccuuuac uuucacaaag     1020

auucccgcag agacccugca cggcacuguc acaguggaag uacaauaugc ugguaccgac     1080

gggccuugua aaguuccagc ccagauggcu guggauaugc agacguuaac cccggucggc     1140

cgacugauca cagccaaucc cgugauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg cauucgaggc caccguuagg     1320

ggcgcuaaac ggauggcggu gcugggggac acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4106
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 1 opt

<400>  4106
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cggugcugac     1080

gggccuugua aaguaccagc ccagauggca guugauaugc agaccuuaac gccggugggc     1140

cgacugauca ccgccaaucc cgucauaaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg cuuucgaggc gacugugagg     1320

ggcgcaaaac ggauggccgu ucugggggac accgcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4107
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 1 opt

<400>  4107
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac acuggagcug acaccggcac accacauugg      780

aauaacaaag aggcccuugu ggaguucaag gaugcgcacg caaagagaca gaccguagug      840

gucuugggcu cccaggaagg ggccgugcau acggcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacug cggccuuuac auucaccaag     1020

auucccgcag agacucugca cggcacagug accgucgaag ugcaauaugc ugguacggac     1080

gggccuugua aaguaccagc ccagauggcu guugauaugc agaccuuaac accggugggc     1140

cgacugauca cugccaaucc cgucauaacc gagggaacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg cgauagcuac auugugaucg gggucggaga aaagaaaauu     1260

acucaucacu ggcauagauc ggguucuacc aucggcaagg cauucgaggc cacagugagg     1320

ggagcuaaac ggauggcggu ucugggggac accgcauggg auuuuggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4108
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 1 opt

<400>  4108
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagc aauggcccaa      180

gacaaaccua cuguugauau cgaguuggug acgaccacag ucaguaauau ggcggaagug      240

cggagcuacu gcuaugaggc uuccauuucu gacauggccu cggauucacg cuguccaacu      300

cagggagaag cauacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gccaaauucg cuuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugcuggu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcaaag guggaaauca cgcccaacag uccucgggcc      600

gaggcuacuc ucgggggauu uggcagcuug ggucuggauu gcgagccacg caccgggcug      660

gacuucucug aucucuauua cuuaacaaug aauaacaaac acuggcuggc ccauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gcgggagcag ccacuggcac cccacauugg      780

aauaacaaag aggcuuuggu cgaguucaag gacgcccacg caaagagaca gacaguugug      840

guacugggcu cccaggaagg ggcugugcau accgcccuag cgggagcccu cgaagcagag      900

auggauggug cuaaaggcag gcugucaagc gggcaccuua agugucggcu gaaaauggac      960

aaguugcgcc ucaaaggagu caguuacucu cugugcacgg ccgcuuuuac uuucacaaag     1020

auccccgccg agaccgugga uggcacuguu acaguggaag gucaauaugg gggcaccgac     1080

ggaccuugua aagucccagc acagauggcc guggauaugc agacguuaac cccgguaggc     1140

cgacugauua cagcuaaucc cguuaucacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg ggauucguac auagugauug gagucgguga aaagaaaauc     1260

acacaccauu ggcacagauc uggcuccacu auugggaagg cguucgaggc aaccgugagg     1320

ggagccaaac ggauggcugu ccugggcgac acagccuggg auuuugguuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4109
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 1 opt

<400>  4109
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccug ccguggauau cgaguugguc acuacgaccg ugaguaauau ggcggaagua      240

cggagcuacu gcuaugaggc uuccauuucu gacauggccu cggauucacg cuguccaaca      300

cagggagaag cauacuuaga caagcagagc gauacucagu auguuugcaa acgaacccug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgucacaugc      420

gccaaauucg cuuguuccaa gaagaugacc gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu gcauggaucc cagcacucag guaugaucgu gaacgauacu      540

ggccaugaaa cagacgagaa uagggcaaag gucgaaauua cccccaacag uccucgggcc      600

gaggcuacgc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacugggcug      660

gacuucucug aucuguauua ccucaccaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcgg acacaggcac uccacauugg      780

aauaacaaag aggcacuugu ggaguucaag gaugcccacg cuaagagaca gaccguagug      840

gucuugggcu cccaggaagg ggccgugcau acagcacugg cuggagcccu agaagcggag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcaccg cagcuuuuac guucacuaag     1020

auucccgccg agacacugca cggcaccgug acugucgaag ugcaauaugc ugguacagac     1080

gggccuugua aaguaccagc ccagauggca guugauaugc agaccuuaac gccggugggc     1140

cgacugauca ccgccaaucc cgucauaaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg cuuucgaggc gacugugagg     1320

ggcgcaaaac ggauggccgu ucugggggac accgcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4110
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 1 opt

<400>  4110
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu aacaauaggg acuucgugga ggggaugucu      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugucaaacau ggccgaagua      240

cggaguuacu gcuaugaggc gagcauuucc gacauggcuu cugauucgcg cuguccaacu      300

cagggagaag ccuacuuaga caagcaguca gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa ugggugugga cuauuuggca agggcagccu cgugaccugc      420

gcaaaauucg ccuguaguaa gaagaugacu gggaaaucca uccaaccgga gaaccuggag      480

uacagaauaa ugcuuucugu ccauggaagc cagcacuccg guaugaucgu gaaugauaca      540

ggccaugaaa ccgacgagaa cagggcuaag gucgaaauua cgcccaauuc accucgggca      600

gaggccacuc ugggaggguu uggcaguuug ggucucgauu gcgagccacg caccgggcug      660

gacuucagcg aucuguauua ccucacaaug aacaauaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aacaauaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cucaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucuccuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uagcuacagu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaccc cgucauaacu gagucuaccg aaaauuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauagcuac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc gggaucuacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4111
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 1 opt

<400>  4111
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcgccg cagcuuuuac guucacuaag     1020

auucccgccg agacacugca cggcaccgug acugucgaag ugcaauaugc ugguacagac     1080

gggccuugua aaguaccagc ccagauggca guugauaugc agaccuuaac gccggugggc     1140

cgacugauca ccgccaaucc cgucauaaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg cuuucgaggc gacugugagg     1320

ggcgcaaaac ggauggccgu ucugggggac accgcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4112
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 1 opt

<400>  4112
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauauu      540

ggccaugaaa cagacgagaa uagggcuaag gucgaaauca cccccaacag uccucgggca      600

gaggccacgc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacugggcug      660

gacuucucug aucuguauua ccucaccaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacaggcac uccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gaccguagug      840

gucuugggcu cccaggaagg ggcugugcau acagcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcaccg ccgcauuuac guucacuaag     1020

auccccgcug agacacugca cggcaccgug acugucgaag ugcaauaugc cgguacagac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agaccuuaac gccggugggc     1140

cgacugauaa ccgcaaaucc cgucauuaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgugauug gcgucgggga caagaaaauc     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg ccuucgaagc uacugugagg     1320

ggcgcgaaac ggauggcagu ucugggggau accgccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4113
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 1 opt

<400>  4113
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga caagaaaauu     1260

acacaucacu gguauagauc uggauccacu aucgguaagg ccuucgaagc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggau acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4114
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 1 opt

<400>  4114
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauacc      540

ggccaugaaa cggacgagaa uagggccaag gucgaaguua cucccaacag uccucgggcu      600

gaggcaaccc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacagggcug      660

gacuucucug aucuguauua ccucacuaug aauaacaaac acugguuagu gcauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gccggagcug acaccggcac accacauugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcacg caaagagaca gaccgugguc      840

guguugggcu cccaggaagg ggccguucau acggcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucuuuuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gagcuacagu cucugcacug cggccuucac auuuaccaag     1020

guccccgcag agacucugca cggcacagug accguagaag uucaauaugc ugguacggac     1080

gggccuugua aagugccagc ccagauggcu gucgauaugc agaccuuaac accggugggc     1140

cgacugauca cugccaaucc cgucauuacc gagucuacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuac aucgugauag gcguugggga caagaaaauu     1260

acucaucacu ggcauagauc gggaucuacc aucgguaagg cauuugaagc cacagugagg     1320

ggcgcuaaac ggauggcggu acugggggau accgcauggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4115
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 1 opt

<400>  4115
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauauu      540

ggccaugaaa ccgacgagaa uagggccaag gucgaaguua cgcccaacag uccucgggcu      600

gaggcaacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu gcauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcug acacuggcac cccacauugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcacg caaagagaca gacagugguc      840

guguugggcu cccaggaagg ggccguucau accgcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucuuuuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gagcuacagu cucugcacgg cggccuucac uuuuacaaag     1020

guccccgcag agacccugca cggcacugug acaguagaag uucaauaugc ugguaccgac     1080

gggccuugua aagugccagc ccagauggcu gucgauaugc agacguuaac cccggugggc     1140

cgacugauua cagccaaucc cgucaucacu gagucuaccg aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuac auagugauug gcguugggga caagaaaauc     1260

acacaucacu ggcauagauc gggaucuacu auugguaagg cauuugaagc caccgugagg     1320

ggcgcuaaac ggauggcggu acugggggau acagcauggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4116
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 1 opt

<400>  4116
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauauu      540

ggccaugaaa ccgacgagaa uagggccaag gucgaaguua cgcccaacag uccucgggcu      600

gaggcaacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu gcauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcug acacuggcac cccacauugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcacg caaagagaca gacagugguc      840

guguugggcu cccaggaagg ggccguucau accgcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucuuuuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gagcuacagu cucugcacgg cggucuguac ugccgcaaag     1020

gugcccgcug agacacugca cggcaccgua acuguugaag ugcaauaugc cgguacagac     1080

gggccuugca aagucccagc ucagauggcc guggauaugc agaccuuaac gccggucggc     1140

cgacugauua ccgcaaaucc cgugaucaca gagucuacug aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuac auaguuauug gcguggggga caagaaaauc     1260

acccaucacu ggcauagauc gggaucuaca auugguaagg ccuuugaagc uacuguaagg     1320

ggcgcgaaac ggauggcagu ccugggggau accgccuggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4117
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 1 opt

<400>  4117
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgaugaa      540

aauagggcca aggucgaggu uacccccaac aguccucggg cugaagcaac gcugggcggg      600

uuuggcagcu ugggacucga cugcgagcca cgcacugguc uggauuucuc ugaccuguau      660

uaccucacca ugaauaacaa acauugguua gugcacaagg agugguuuca ugauauuccc      720

cugccuuggc acuccggggc cgacacagaa acuccacacu ggaauaacaa agaggcucuu      780

guagaguuca aggacgccca ugcgaagaga cagaccgugg ucguguuggg aucacaggaa      840

ggcgcaguuc acacagcccu ggcuggcgcc cuagaagcag agauggaugg ggcuaaagga      900

aggcucagca guggucaucu gaagugucgg cuuaaaaugg acaagcugcg cuugaaaggc      960

gugucuuacu cccucugcac cgccgcguuu acguucacua agguccccgc cgagacacug     1020

cacgggaccg ugacuguaga aguucaauau gcaggacgag auggcccuug uaaagugcca     1080

gcucagaugg ccgucgacau gcagacauua accccggugg guagacugau cacggcuaau     1140

cccgucauua ccgagagcac agaaaacucg aagaugaugc ucgagcuuga uccuccauuu     1200

ggggacucuu acaucgugau aggcguugga gauaagaaaa uuacucacca uuggcacagg     1260

uccggcucaa ucauugggaa ggccuucgaa gcaaccgugc ggggagccaa acguauggcu     1320

guacugggug acacagcgug ggauuuuggc aguugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  4118
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 1 opt

<400>  4118
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauugu ucucauagug       60

cccgccuauu ccaucucuug cauuggcgua ucaaacagag accuugugga ggggaugagu      120

ggagguaccu gggucgaugu gguucuggaa cacggcgggu gugugacaga gauggcucaa      180

gacaaaccua cugucgauau cgaauuggug acgaugaccg uaagcaauau ggcagagguu      240

agguccuacu gcuaugaagc cucuuuaucg gacauggcgu cagcuagccg guguccaaca      300

cagggagagc cgagucugga uaagcagucc gacacucagu cugugugcaa acgcacccua      360

ggcgaucgag guugggggaa cggauguggc auuuucggca aggggagccu cgucacaugc      420

uccaaauuua ccuguugcaa gaagaugccc ggaaaaucaa uccaaccuga gaaucuggaa      480

uaccguauaa ugcuuccagu gcaugguagu cagcacagcg gcaugaucgu gaacgacauu      540

gggcaugaga cugaugaaaa uagagccaag gucgagguua cacccaacuc uccuagggca      600

gaggccaccc ugggaggcuu cgguuccuug gggcucgacu gugaaccacg gacgggacug      660

gauuuuucag accuguauua ccucacuaug aauaacaaac acugguuagu gcauaaggag      720

ugguuccacg auaucccccu gccuuggcac gcuggcgcag acaccggcac accacauugg      780

aauaacaaag aagcccuugu agaguuuaag gacgcucacg ccaagcgcca gacugugguc      840

guguugggga gccaggaagg agcgguucau accgcacugg ccggugcucu agaggccgag      900

auggauggcg caaaagggag acucuucagu ggacaccuga agugcaggcu uaaaauggac      960

aagcugcggu ugaaaggcgu gucuuauucc cucuguacag cugccuuuac cuucacgaag     1020

gucccggcgg aaacucugca cgguacagug accguagagg uucaaagcgc cgggacugau     1080

ggccccugca aagugccugc acagauggcu gucgacaugc agacauuaac cccaguggga     1140

cgccugauua cggccaaucc cgucaucacc gaaucgacag agaacucuaa gaugaugcuc     1200

gaacuugauc cuccauuugg cgacuccuac auagugauug ggguuggaga uaagaaaauc     1260

acucaucacu ggcaucgauc agguaguacc auuggcaagg cuuucgaggc cacagugaga     1320

ggggcaaaaa ggauggccgu acugggagac acugcuuggg auuuuggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4119
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 1 opt

<400>  4119
gggagaaagc uuaccaugag acagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuaua gcaucaggug cauuggcguu uccaaccggg acuucgugga ggggaugucu      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugucaaauau ggccgaagua      240

cgcaguuacu gcuaugaggc gagcauuucc gacauggcuu cugauucgcg auguccaacu      300

cagggagaag ccuacuuaga caagcaguca gauacccagu auguuugcaa acguacacug      360

guggacagag gcugggguaa cgggugugga cuauuuggca agggcagccu cgugaccugc      420

gcaaaauucg ccuguaguaa gaagaugacu gggaaaucca uccaaccgga gaaucuggag      480

uacaggauaa ugcuuucugu ccauggaagc cagcacuccg guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa ucgggcuaag gucgaaauua cgcccaacuc accucgcgca      600

gaggccacuc ugggaggguu uggcaguuug ggucucgauu gcgagccaag aaccgggcug      660

gacuucagcg aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagaggca gacaguagug      840

gucuugggcu cucaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggccg gcucuccuca gggcaccuga agugucgccu uaaaauggau      960

aagcugcgau ugaaaggagu uagcuacagu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

agacugauca cagcaaaucc cgucauaacu gagucuaccg aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauagcuac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagguc gggaucuacu aucgguaagg ccuucgaggc uaccgugcgg     1320

ggcgcgaaac guauggcagu ucugggggac acagccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4120
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 1 opt

<400>  4120
gggagaaagc uuaccaugag ccagagagug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucaggug cauuggcguu ucuaaccggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaagccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cgcagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg auguccaacu      300

cagggagaag ccuacuuaga caaacagagc gauacccagu auguuugcaa gcguacacug      360

guggacagag gcugggguaa cgggugugga cuauuuggca aaggcagucu cgugaccugc      420

gcaaaguuca cuuguuccaa aaagaugaca gggaagucua uccaaccgga gaaucuggag      480

uacaggauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauauu      540

ggccaugaaa ccgacgagaa ucgggccaaa gucgaaguua cgcccaacag uccucgcgcu      600

gaggcaacuc ugggaggguu uggcagcuug ggucucgauu gcgagccaag aaccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaagc acugguuagu gcauaaagaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcug acacuggcac cccacauugg      780

aauaacaagg aggcccuugu agaguucaaa gaugcgcacg caaagaggca gacagugguc      840

guguugggcu cccaggaagg ggccguucau accgcucugg ccggagcacu agaagcugag      900

auggacggug ccaagggccg gcucuuuuca gggcaccuga aaugucgcau uaagauggau      960

aaacuucgac ugaagggagu gagcuacagu uugugcacgg cggccuucac uuuuucuaaa     1020

guccccgcag agacacucca cggcaccgug acuguagaag uucaauaugc ugguagagac     1080

gggccuugua aggugccagc ccagauggcu gucgauaugc agacacugac cccggugggc     1140

agguuaauca cggccaaucc cauaauuacc gaguccacag aaaacagccg gaugaugcug     1200

gagcucgacc cuccauucgg agauucguac aucgucauug gcguggggga caaaaagauc     1260

acucaucacu ggcaucguuc uggauccacc aucgguaagg cauuugaagc cacaguucgc     1320

ggcgcuaaaa gaauggcggu gcuuggggau acugcauggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4121
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  4121
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4122
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 opt

<400>  4122
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4123
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 opt

<400>  4123
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1080

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4124
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 1 opt

<400>  4124
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4125
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  4125
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4126
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 1 opt

<400>  4126
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4127
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 1 opt

<400>  4127
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4128
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 1 opt

<400>  4128
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4129
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 opt

<400>  4129
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4130
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 opt

<400>  4130
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4131
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 opt

<400>  4131
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      540

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug      600

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc      840

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      900

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug      960

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1140

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1320

auggccgugc ugggcgacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4132
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 1 opt

<400>  4132
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4133
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       1 opt

<400>  4133
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccugag cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4134
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 1
       opt

<400>  4134
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccugag cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4135
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 1 
       opt

<400>  4135
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccugag cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4136
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 1 
       opt

<400>  4136
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccugag cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4137
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       1 opt

<400>  4137
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugcagg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4138
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 1
       opt

<400>  4138
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugcagg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4139
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 1 
       opt

<400>  4139
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugcagg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4140
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4140
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4141
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4141
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4142
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 1 opt

<400>  4142
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc acccugggca aggccuucag caccacccug     1800

aagggcgccc agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc     1860

ggcguguuca acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc     1920

cuguucggcg gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug     1980

ggcgugaacg ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug     2040

guguuccugg ccaccaacgu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4143
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4143
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4144
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  4144
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4145
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4145
gggagaaagc uuaccaugau caucaacgcc agaaaggaga agaagagaag aggcgccgac       60

accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga      120

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      180

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      360

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      420

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      480

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      540

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      600

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      660

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      720

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      780

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      840

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      900

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac     1020

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1080

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1140

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1200

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1380

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1440

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1500

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1620

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1680

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1740

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1800

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1860

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1920

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     2040

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2100

cugagcaccg ccgugagcgc cgacgugggc ugcagcgugg acuucagcaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  4146
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4146
gggagaaagc uuaccaugau caacgccaga aaggagaaga agagaagagg cgccgacacc       60

agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga      120

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      180

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      240

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      360

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      420

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      480

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      540

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      600

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      660

accugggugg acaucgugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      720

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      780

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      840

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      900

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      960

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga     1020

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1080

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1140

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1200

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1380

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1440

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1500

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1620

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1680

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1740

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1800

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1860

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1920

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1980

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     2040

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2100

agcaccgccg ugagcgccga cgugggcugc agcguggacu ucagcaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  4147
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4147
gggagaaagc uuaccaugaa cgccagaaag gagaagaaga gaagaggcgc cgacaccagc       60

gugggcaucg ugggccugcu gcugaccacc gccauggccg ccgaggugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcagc guggacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4148
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4148
gggagaaagc uuaccauggc cagaaaggag aagaagagaa gaggcgccga caccagcgug       60

ggcaucgugg gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc      120

gccuacuaca uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc      180

cugggcauga acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc      240

augagcuacg agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc      360

agaagaagca gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      420

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      480

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      540

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      600

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      660

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      720

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      780

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      840

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      900

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug     1020

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc     1080

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4149
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4149
gggagaaagc uuaccaugag aaaggagaag aagagaagag gcgccgacac cagcgugggc       60

aucgugggcc ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc      120

uacuacaugu accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug      180

ggcaugaaca agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug      240

agcuacgagu gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga      360

agaagcagaa gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      420

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      480

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      540

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      600

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      660

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      720

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      780

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      840

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      900

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      960

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug     1020

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac     1080

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc     1140

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1200

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1380

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1440

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1500

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1620

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1680

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1740

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1800

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1860

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1980

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     2040

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     2100

gugagcgccg acgugggcug cagcguggac uucagcaagu gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  4150
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4150
gggagaaagc uuaccaugaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4151
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4151
gggagaaagc uuaccaugga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgacgugg gcugcagcgu ggacuucagc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  4152
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4152
gggagaaagc uuaccaugaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gacgugggcu gcagcgugga cuucagcaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  4153
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4153
gggagaaagc uuaccaugaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugca gcguggacuu cagcaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4154
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4154
gggagaaagc uuaccaugag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcagcg uggacuucag caagugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4155
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4155
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcagcgugg acuucagcaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4156
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4156
gggagaaagc uuaccauggg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

agcguggacu ucagcaagug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4157
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4157
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcagc     2100

guggacuuca gcaagugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4158
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4158
gggagaaagc uuaccaugga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     2100

gacuucagca agugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4159
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4159
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cagcguggac     2100

uucagcaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4160
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4160
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgccg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cagcguggac     2100

uucagcaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4161
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4161
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccgc cgugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcagcgugg acuucagcaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4162
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4162
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcggcgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcagc guggacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4163
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4163
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcgccgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcagc guggacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4164
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4164
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4165
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4165
gggagaaagc uuaccaugau caucaacgcc agaaaggaga agaagagaag aggcgccgac       60

accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga      120

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      180

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      360

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      420

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      480

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      540

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      600

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      660

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      720

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      780

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      840

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      900

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac     1020

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1080

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1140

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1200

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1380

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1440

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1500

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1620

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1680

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1740

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1800

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1860

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1920

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     2040

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2100

cugagcaccg ccgugagcgc cgacgugggc ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  4166
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4166
gggagaaagc uuaccaugau caacgccaga aaggagaaga agagaagagg cgccgacacc       60

agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga      120

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      180

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      240

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      360

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      420

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      480

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      540

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      600

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      660

accugggugg acaucgugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      720

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      780

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      840

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      900

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      960

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga     1020

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1080

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1140

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1200

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1380

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1440

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1500

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1620

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1680

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1740

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1800

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1860

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1920

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1980

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     2040

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2100

agcaccgccg ugagcgccga cgugggcugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  4167
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4167
gggagaaagc uuaccaugaa cgccagaaag gagaagaaga gaagaggcgc cgacaccagc       60

gugggcaucg ugggccugcu gcugaccacc gccauggccg ccgaggugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4168
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4168
gggagaaagc uuaccauggc cagaaaggag aagaagagaa gaggcgccga caccagcgug       60

ggcaucgugg gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc      120

gccuacuaca uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc      180

cugggcauga acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc      240

augagcuacg agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc      360

agaagaagca gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      420

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      480

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      540

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      600

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      660

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      720

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      780

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      840

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      900

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug     1020

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc     1080

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4169
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4169
gggagaaagc uuaccaugag aaaggagaag aagagaagag gcgccgacac cagcgugggc       60

aucgugggcc ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc      120

uacuacaugu accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug      180

ggcaugaaca agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug      240

agcuacgagu gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga      360

agaagcagaa gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      420

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      480

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      540

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      600

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      660

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      720

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      780

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      840

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      900

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      960

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug     1020

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac     1080

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc     1140

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1200

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1380

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1440

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1500

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1620

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1680

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1740

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1800

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1860

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1980

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     2040

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     2100

gugagcgccg acgugggcug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4170
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4170
gggagaaagc uuaccaugaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4171
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4171
gggagaaagc uuaccaugga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgacgugg gcugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4172
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4172
gggagaaagc uuaccaugaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gacgugggcu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4173
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4173
gggagaaagc uuaccaugaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4174
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4174
gggagaaagc uuaccaugag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4175
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4175
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4176
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4176
gggagaaagc uuaccauggg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4177
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4177
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4178
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4178
gggagaaagc uuaccaugga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4179
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4179
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4180
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4180
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgccg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4181
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4181
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccgc cgugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4182
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4182
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcggcgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4183
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4183
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcgccgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4184
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4184
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4185
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4185
gggagaaagc uuaccaugau caucaacgcc agaaaggaga agaagagaag aggcgccgac       60

accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga      120

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      180

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      360

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      420

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      480

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      540

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      600

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      660

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      720

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      780

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      840

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      900

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac     1020

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1080

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1140

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1200

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1380

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1440

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1500

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1620

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1680

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1740

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1800

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1860

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1920

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     2040

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2100

cugagcaccg ccgugagcgc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4186
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4186
gggagaaagc uuaccaugau caacgccaga aaggagaaga agagaagagg cgccgacacc       60

agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga      120

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      180

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      240

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      360

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      420

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      480

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      540

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      600

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      660

accugggugg acaucgugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      720

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      780

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      840

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      900

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      960

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga     1020

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1080

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1140

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1200

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1380

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1440

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1500

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1620

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1680

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1740

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1800

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1860

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1920

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1980

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     2040

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2100

agcaccgccg ugagcgccga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4187
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4187
gggagaaagc uuaccaugaa cgccagaaag gagaagaaga gaagaggcgc cgacaccagc       60

gugggcaucg ugggccugcu gcugaccacc gccauggccg ccgaggugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4188
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4188
gggagaaagc uuaccauggc cagaaaggag aagaagagaa gaggcgccga caccagcgug       60

ggcaucgugg gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc      120

gccuacuaca uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc      180

cugggcauga acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc      240

augagcuacg agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc      360

agaagaagca gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      420

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      480

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      540

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      600

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      660

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      720

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      780

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      840

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      900

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug     1020

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc     1080

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     2100

gccgugagcg ccgacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4189
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4189
gggagaaagc uuaccaugag aaaggagaag aagagaagag gcgccgacac cagcgugggc       60

aucgugggcc ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc      120

uacuacaugu accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug      180

ggcaugaaca agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug      240

agcuacgagu gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga      360

agaagcagaa gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      420

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      480

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      540

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      600

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      660

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      720

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      780

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      840

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      900

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      960

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug     1020

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac     1080

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc     1140

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1200

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1380

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1440

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1500

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1620

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1680

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1740

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1800

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1860

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1980

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     2040

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     2100

gugagcgccg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4190
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4190
gggagaaagc uuaccaugaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4191
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4191
gggagaaagc uuaccaugga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4192
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4192
gggagaaagc uuaccaugaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4193
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4193
gggagaaagc uuaccaugaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4194
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4194
gggagaaagc uuaccaugag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4195
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4195
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4196
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4196
gggagaaagc uuaccauggg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4197
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4197
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4198
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4198
gggagaaagc uuaccaugga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  4199
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4199
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4200
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4200
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgccg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4201
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4201
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccgc cgugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4202
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4202
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcggcgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4203
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4203
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcgccgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4204
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4204
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4205
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4205
gggagaaagc uuaccaugau caacgccaga aaggagaaga agagaagagg cgccgacacc       60

agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga      120

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      180

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      240

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      360

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      420

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      480

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      540

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      600

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      660

accugggugg acaucgugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      720

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      780

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      840

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      900

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      960

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga     1020

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1080

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1140

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1200

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1380

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1440

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1500

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1620

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1680

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1740

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1800

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1860

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1920

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1980

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     2040

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2100

agcaccgccg ugagcgccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4206
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4206
gggagaaagc uuaccaugau caucaacgcc agaaaggaga agaagagaag aggcgccgac       60

accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga      120

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      180

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      360

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      420

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      480

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      540

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      600

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      660

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      720

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      780

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      840

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      900

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac     1020

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1080

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1140

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1200

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1380

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1440

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1500

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1620

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1680

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1740

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1800

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1860

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1920

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     2040

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2100

cugagcaccg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4207
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4207
gggagaaagc uuaccaugaa cgccagaaag gagaagaaga gaagaggcgc cgacaccagc       60

gugggcaucg ugggccugcu gcugaccacc gccauggccg ccgaggugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4208
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4208
gggagaaagc uuaccauggc cagaaaggag aagaagagaa gaggcgccga caccagcgug       60

ggcaucgugg gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc      120

gccuacuaca uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc      180

cugggcauga acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc      240

augagcuacg agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc      360

agaagaagca gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      420

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      480

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      540

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      600

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      660

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      720

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      780

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      840

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      900

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug     1020

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc     1080

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     2100

gccgugagcg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4209
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4209
gggagaaagc uuaccaugag aaaggagaag aagagaagag gcgccgacac cagcgugggc       60

aucgugggcc ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc      120

uacuacaugu accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug      180

ggcaugaaca agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug      240

agcuacgagu gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga      360

agaagcagaa gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      420

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      480

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      540

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      600

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      660

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      720

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      780

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      840

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      900

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      960

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug     1020

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac     1080

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc     1140

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1200

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1380

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1440

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1500

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1620

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1680

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1740

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1800

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1860

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1980

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     2040

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     2100

gugagcgccu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4210
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4210
gggagaaagc uuaccaugaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4211
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4211
gggagaaagc uuaccaugga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4212
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4212
gggagaaagc uuaccaugaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4213
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4213
gggagaaagc uuaccaugaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4214
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4214
gggagaaagc uuaccaugag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4215
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4215
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4216
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4216
gggagaaagc uuaccauggg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4217
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4217
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  4218
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4218
gggagaaagc uuaccaugga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4219
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  4219
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4220
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4220
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgccg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4221
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4221
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccgc cgugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4222
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4222
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcggcgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4223
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4223
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcgccgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4224
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 1 opt

<400>  4224
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4225
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 1 opt

<400>  4225
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4226
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 1 opt

<400>  4226
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1080

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4227
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 1 opt

<400>  4227
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4228
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 1 opt

<400>  4228
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4229
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 1 opt

<400>  4229
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucacccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4230
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 1 opt

<400>  4230
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccacagaaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4231
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 1 opt

<400>  4231
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaccgu gaacgacauc     1080

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4232
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 1 opt

<400>  4232
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1080

gacgagaaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcaagc uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4233
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 1 opt

<400>  4233
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcag aggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4234
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 1 opt

<400>  4234
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacgccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4235
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 1 opt

<400>  4235
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc cuggcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4236
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 1 opt

<400>  4236
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu gguacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4237
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 1 opt

<400>  4237
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgagauc      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4238
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 1 opt

<400>  4238
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcuggc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4239
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 1 opt

<400>  4239
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc acccuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4240
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 1 opt

<400>  4240
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccguggugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4241
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 1 opt

<400>  4241
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcgcc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaccccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4242
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 1 opt

<400>  4242
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag caagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4243
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 1 opt

<400>  4243
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag acagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4244
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 1 opt

<400>  4244
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaagcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaccc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4245
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 1 opt

<400>  4245
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccaacg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccagaa gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4246
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 1 opt

<400>  4246
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gagaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4247
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 1 opt

<400>  4247
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gccugagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4248
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 1 opt

<400>  4248
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccaacg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggauguucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4249
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 1 opt

<400>  4249
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggauccugag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcaucggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4250
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 1 opt

<400>  4250
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug agcaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc caccaucagc gacaucgcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccgccgug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4251
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 1 opt

<400>  4251
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca cccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4252
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 1 opt

<400>  4252
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagc ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4253
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 1 opt

<400>  4253
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gagaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

aucgagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4254
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 1 opt

<400>  4254
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaucg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4255
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 1 opt

<400>  4255
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ugaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagcuuc augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4256
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 1 opt

<400>  4256
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4257
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 1 opt

<400>  4257
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gaggcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4258
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 1 opt

<400>  4258
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcgccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4259
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 1 opt

<400>  4259
gggagaaagc uuaccaugag aaucaucaac gccggcaagg agaagaagag aagaggcgcc       60

gacaccaacg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccagaa gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4260
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 1 opt

<400>  4260
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gagaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaagg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4261
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 1 opt

<400>  4261
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacaugguga gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4262
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 1 opt

<400>  4262
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccaacg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacau gaccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4263
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 1 opt

<400>  4263
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccgccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagacc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4264
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 1 opt

<400>  4264
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gagaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cuggccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4265
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 1 opt

<400>  4265
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcauc agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcauccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4266
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 1 opt

<400>  4266
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caucagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4267
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 1 opt

<400>  4267
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg cccagagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4268
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 1 opt

<400>  4268
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcaggcca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4269
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 1 opt

<400>  4269
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4270
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 1 opt

<400>  4270
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac accggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagggcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gugcugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4271
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 1 opt

<400>  4271
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgc cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugcuggu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggc ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg ccaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agaccgugga cggcaccgug accguggagg gccaguacgg cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucaucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cggcggcgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4272
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 1 opt

<400>  4272
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucagcaag     1560

auccccgccg agacccugca cggcaccgug accgaggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4273
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 1 opt

<400>  4273
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4274
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 1 opt

<400>  4274
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca acgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca ccaugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4275
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 1 opt

<400>  4275
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug gugugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4276
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 1 opt

<400>  4276
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagcccg ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4277
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 1 opt

<400>  4277
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgu gaucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4278
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 1 opt

<400>  4278
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcgu gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu gguacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4279
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 1 opt

<400>  4279
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uuccccacca cccugggcgu gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4280
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 1 opt

<400>  4280
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucacccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4281
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 1 opt

<400>  4281
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca accugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcgugcac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4282
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 1 opt

<400>  4282
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4283
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 1 opt

<400>  4283
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca accugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4284
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 1 opt

<400>  4284
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4285
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 1 opt

<400>  4285
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccagcacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgugugcac cgccgccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4286
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 1 opt

<400>  4286
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacgccagca ucggcaucgu gagccugcug cugaccaccg ugauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgugacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acagcggcgc cgacaccgag accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcagag acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ucaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4287
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 1 opt

<400>  4287
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4288
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 1 opt

<400>  4288
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgaga ccagaagaag cagaagaagc gugagccuga gauaccacua caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaagaagca ccugaucaug      480

guggagaacu ggaucuucag aaaccccggc uucgccaucg ugagcguggc caucaccugg      540

cugaugggca gccugaccag ccagaaggug aucuaccugg ugaugaucgu gcugaucgug      600

cccgccuaca gcaucagcug caucggcgug agcaacagag accuggugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccga gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaugaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagccugagc gacauggcca gcgccagcag augccccacc      840

cagggcgagc ccagccugga caagcagagc gacacccaga gcgugugcaa gagaacccug      900

ggcgacagag gcuggggcaa cggcugcggc aucuucggca agggcagccu ggugaccugc      960

agcaaguuca ccugcugcaa gaagaugccc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugcccgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcagagcgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4289
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 1 opt

<400>  4289
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca ccaugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagacccag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggauccugag aaaccccggc uucaccgugg ccgcccuggc cauggccugg      540

cugcugggca acagcaccag ccagagagug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagaau caagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucagcaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcagagac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc caucaucacc gagagcaccg agaacagcag aaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcgugcac cagguguucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug gccugccugg cccugggcgg cgccaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  4290
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 1 opt

<400>  4290
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4291
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 1 opt

<400>  4291
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4292
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 1 opt

<400>  4292
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4293
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 1 opt

<400>  4293
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4294
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 1 opt

<400>  4294
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcua cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcaagcugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaagauc cccgugcaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4295
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 1 opt

<400>  4295
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccaca gaaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcua cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcaagcugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaagauc cccgugcaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4296
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 1 opt

<400>  4296
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

accgugaacg acaucggcua cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcaagcugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaagauc cccgugcaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4297
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 1 opt

<400>  4297
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucac ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4298
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 1 opt

<400>  4298
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg caagcuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4299
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 1 opt

<400>  4299
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcagaggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4300
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 1 opt

<400>  4300
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggccuggc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4301
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 1 opt

<400>  4301
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacugguac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4302
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 1 opt

<400>  4302
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga cacccagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4303
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 1 opt

<400>  4303
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg agaucagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4304
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 1 opt

<400>  4304
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cuggcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4305
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 1 opt

<400>  4305
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccacccu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4306
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 1 opt

<400>  4306
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgug gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4307
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 1 opt

<400>  4307
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcgccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaccc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4308
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 1 opt

<400>  4308
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagcaaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4309
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 1 opt

<400>  4309
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagacaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4310
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 1 opt

<400>  4310
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcacccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4311
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 1 opt

<400>  4311
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagccug agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4312
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 1 opt

<400>  4312
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagaaucga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4313
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 1 opt

<400>  4313
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc cugagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcauc     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4314
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 1 opt

<400>  4314
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugagcac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccacca ucagcgacau cgccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ccguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4315
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 1 opt

<400>  4315
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggagcugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4316
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 1 opt

<400>  4316
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caucgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4317
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 1 opt

<400>  4317
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgugaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gcuucaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4318
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 1 opt

<400>  4318
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcaagcuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4319
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 1 opt

<400>  4319
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaaggacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4320
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 1 opt

<400>  4320
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggaggc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4321
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 1 opt

<400>  4321
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cgcccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agacccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4322
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 1 opt

<400>  4322
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggcg ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4323
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 1 opt

<400>  4323
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccuggc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4324
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 1 opt

<400>  4324
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggugagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4325
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 1 opt

<400>  4325
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcaucagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca uccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4326
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 1 opt

<400>  4326
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcauca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4327
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 1 opt

<400>  4327
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggcccag agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4328
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 1 opt

<400>  4328
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca ggccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4329
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 1 opt

<400>  4329
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca gcaagauccc cgccgagacc cugcacggca ccgugaccga ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4330
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 1 opt

<400>  4330
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacaccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgaggg caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgugcu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4331
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 1 opt

<400>  4331
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4332
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 1 opt

<400>  4332
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcaacgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccaccaug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4333
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 1 opt

<400>  4333
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccuggugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4334
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 1 opt

<400>  4334
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugaacaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccuggugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4335
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 1 opt

<400>  4335
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccgccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4336
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 1 opt

<400>  4336
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgugaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4337
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 1 opt

<400>  4337
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcgugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacugguac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4338
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 1 opt

<400>  4338
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuuccc caccacccug ggcgugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4339
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 1 opt

<400>  4339
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucac ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4340
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 1 opt

<400>  4340
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccaaccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggcg ugcaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4341
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 1 opt

<400>  4341
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4342
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 1 opt

<400>  4342
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccaaccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4343
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 1 opt

<400>  4343
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4344
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 1 opt

<400>  4344
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccagc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgug     1500

ugcaccgccg ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4345
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 1 opt

<400>  4345
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4346
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 1 opt

<400>  4346
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgagaccaga agaagcagaa gaagcgugag ccugagauac      360

cacuacacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacaag      420

aagcaccuga ucauggugga gaacuggauc uucagaaacc ccggcuucgc caucgugagc      480

guggccauca ccuggcugau gggcagccug accagccaga aggugaucua ccuggugaug      540

aucgugcuga ucgugcccgc cuacagcauc agcugcaucg gcgugagcaa cagagaccug      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgagaugg cccaggacaa gcccaccgug gacaucgagc uggugaccau gaccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagcc ugagcgacau ggccagcgcc      780

agcagaugcc ccacccaggg cgagcccagc cuggacaagc agagcgacac ccagagcgug      840

ugcaagagaa cccugggcga cagaggcugg ggcaacggcu gcggcaucuu cggcaagggc      900

agccugguga ccugcagcaa guucaccugc ugcaagaaga ugcccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug cccgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

agcgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4347
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 1 opt

<400>  4347
gggagaaagc uuaccaugag cgugggcauc gugggccugc ugcugaccac cgccauggcc       60

gccgagguga ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc      120

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      180

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      300

ugccaccaca agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac      360

agcaccagaa agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag      420

caccugauca gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc      480

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      540

cugcugaucg cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug      600

gagggcauga gcggcggcac cuggguggac guggugcugg agcacggcgg cugcgugacc      660

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      720

auggccgagg ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      780

agaugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      840

aagagaaccc ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc      900

cuggugaccu gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc      960

gagaaccugg aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc     1020

gugaacgaca ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac     1080

agccccagag ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc     1140

agaaccggcc uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga     1320

cagaccgugg uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc     1380

cuggaggccg agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga     1440

cugaagaugg acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac     1560

gccggcaccg acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug     1620

acccccgugg gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc     1680

aagaugaugc uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc     1740

gagaagaaga ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag     1800

gccaccguga gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc     1860

agcgugggcg gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug     1980

auguggcugg gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc     2040

ggcgugcuga ucuuccugag caccgccgug agcgccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  4348
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 1 opt

<400>  4348
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc cagaagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4349
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 1 opt

<400>  4349
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggaug uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4350
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 1 opt

<400>  4350
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaugacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4351
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 1 opt

<400>  4351
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgccaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

cuggugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggcccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgccacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc guggacggca ccgugaccgu ggagggccag     1560

uacggcggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau caucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4352
<211>  2275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 1 opt

<400>  4352
gggagaaagc uuaccauggg cgccgacgcc agcaucggca ucgugagccu gcugcugacc       60

accgugaugg ccgccgagau caccagaaga ggcagcgccu acuacaugua ccuggacaga      120

agcgacgccg gcaaggccau cagcuucgug accacccugg gcgugaacaa gugccacgug      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau caagguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cugguggccg uggccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucaccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga cgagaacaga gccaaggugg aggugacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacagcg gcgccgacac cgagaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

agagacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     1740

aagaucaccc accacuggca cagaagcggc agcaucaucg gcaaggccuu cgaggccacc     1800

gugagaggcg ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgugu ucaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcuggugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaccu gccuggcccu gggcggcgug     2040

augaucuucc ugagcaccgc cgugagcgcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2220

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2275


<210>  4353
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 1 opt

<400>  4353
gggagaaagc uuaccauggg cgccgacacc agcaucggca ucaucggccu gcugcugacc       60

accgccaugg ccgccgagau caccagaaga ggcagcgccu acuacaugua ccuggacaga      120

agcgacgccg gcaaggccau cagcuucgcc accaccaugg gcgugaacaa gugccacgug      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagac ccagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau caagguggag aacuggaucc ugagaaaccc cggcuucacc      480

guggccgccc uggccauggc cuggcugcug ggcaacagca ccagccagag agugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucaccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caucggccac gagaccgacg agaacagagc caagguggag     1080

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac     1440

cugaagugca gaaucaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucag caaggugccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcag agacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccaucau caccgagagc     1680

accgagaaca gcagaaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcguguuc aacagccugg gcaagggcgu gcaccaggug     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugguguggcu gggccugaac accaagaacg gcagcaucag ccuggccugc     2040

cuggcccugg gcggcgccau gaucuuccug agcaccgccg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4354
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 1 opt

<400>  4354
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4355
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 1 opt

<400>  4355
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4356
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 1 opt

<400>  4356
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      540

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug      600

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc      840

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      900

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug      960

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1140

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1320

auggccgugc ugggcgacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4357
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 1 opt

<400>  4357
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4358
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 1 opt

<400>  4358
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4359
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 1 opt

<400>  4359
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaccgu gaacgacauc      540

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4360
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 1 opt

<400>  4360
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agcugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  4361
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 1 opt

<400>  4361
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      540

gacgagaaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug      600

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc      840

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      900

aagggcaagc uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug      960

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1140

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1320

auggccgugc ugggcgacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4362
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 1 opt

<400>  4362
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gccugagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4363
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 1 opt

<400>  4363
gggagaaagc uuaccaugag caagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4364
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 1 opt

<400>  4364
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu gguacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4365
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 1 opt

<400>  4365
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcaa gcugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4366
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 1 opt

<400>  4366
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaccc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4367
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 1 opt

<400>  4367
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgagauc      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4368
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 1 opt

<400>  4368
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccagaa gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4369
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 1 opt

<400>  4369
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacaugguga gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4370
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 1 opt

<400>  4370
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaucg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4371
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 1 opt

<400>  4371
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcaucggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4372
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 1 opt

<400>  4372
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cuggccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4373
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 1 opt

<400>  4373
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca cccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4374
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 1 opt

<400>  4374
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcuggc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4375
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 1 opt

<400>  4375
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gaggcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4376
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 1 opt

<400>  4376
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccgccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4377
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 1 opt

<400>  4377
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug agcaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc caccaucagc gacaucgcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccgccgug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4378
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 1 opt

<400>  4378
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucagcaag     1020

auccccgccg agacccugca cggcaccgug accgaggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4379
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 1 opt

<400>  4379
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagc ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4380
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 1 opt

<400>  4380
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcauc agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcauccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4381
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 1 opt

<400>  4381
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ugaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4382
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 1 opt

<400>  4382
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcgccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4383
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 1 opt

<400>  4383
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac accggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagggcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4384
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 1 opt

<400>  4384
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgc cauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugcuggu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggc ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg ccaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agaccgugga cggcaccgug accguggagg gccaguacgg cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4385
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 1 opt

<400>  4385
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagcccg ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4386
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 1 opt

<400>  4386
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug aacaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4387
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 1 opt

<400>  4387
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcgccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4388
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 1 opt

<400>  4388
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4389
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 1 opt

<400>  4389
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu gguacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4390
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 1 opt

<400>  4390
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4391
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 1 opt

<400>  4391
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4392
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 1 opt

<400>  4392
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgugugcac cgccgccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4393
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 1 opt

<400>  4393
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acagcggcgc cgacaccgag accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcagag acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ucaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agcugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  4394
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 1 opt

<400>  4394
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugaucgu gcugaucgug       60

cccgccuaca gcaucagcug caucggcgug agcaacagag accuggugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccga gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaugaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagccugagc gacauggcca gcgccagcag augccccacc      300

cagggcgagc ccagccugga caagcagagc gacacccaga gcgugugcaa gagaacccug      360

ggcgacagag gcuggggcaa cggcugcggc aucuucggca agggcagccu ggugaccugc      420

agcaaguuca ccugcugcaa gaagaugccc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugcccgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcagagcgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4395
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 1 opt

<400>  4395
gggagaaagc uuaccaugag acagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4396
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 1 opt

<400>  4396
gggagaaagc uuaccaugag ccagagagug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagaau caagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucagcaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcagagac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc caucaucacc gagagcaccg agaacagcag aaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4397
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  4397
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4398
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 opt

<400>  4398
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaca      240

ugugacgcca ccaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacacuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaucucagac cuggcucgag agccgggaau acacgaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacauc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcucag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacc      840

caaggagaag ccuaccugga caaacagucu gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcuaaguuug cgugcuccaa gaaaaugacc ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauacg     1080

ggacaugaga cagacgagaa uagagccaaa guggaaauua ccccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auacaggaac gccccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acagcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacgcugca uggcaccgug acaguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cggcgaaccc cguuaucaca gaauccaccg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac acagccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaaugcca agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca ccgccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4399
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 opt

<400>  4399
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgg auacgaaacg     1080

gaugaggaca gagcuaaggu ggaggucaca ccuaauagcc cgcgcgcgga agccacccuu     1140

ggcggcuucg ggucccuggg acucgauugu gagccccgga ccggccugga cuuuucugau     1200

cuuuauuacc ugacaaugaa caauaaacac uggcucgugc auaaggaaug guuccacgac     1260

auuccucugc ccuggcacgc uggggccgau accggaacac cucauuggaa caauaaagag     1320

gcccuggucg aauuuaagga cgcucacgcc aagagacaga cggugguugu gcucggcagc     1380

caggaggggg cgguccauac cgcucuggcc ggcgcccuug aggcugaaau ggauggagcc     1440

aaaggccgcc uguucuccgg gcaccucaag ugccggcuga aaauggacaa acuuagacug     1500

aagggaguga gcuauucucu cuguacagcu gccuuuaccu ucacaaaggu ucccgcggag     1560

acccugcaug gcacggugac cgucgaagug caguacgccg ggacagacgg accgugcaag     1620

aucccugugc aaauggcugu ugauaugcag acccugacac ccgucggccg ccucaucacc     1680

gccaacccug ugauuacgga guccacagaa aauagcaaaa ugaugcugga gcuugacccu     1740

cccuuugggg auucuuacau cgugauuggc gucggagaca agaaaaucac ccaccacugg     1800

caucgguccg gcagcacaau ugggaaggcu uucgaggcca ccgugagagg agcgaaacgc     1860

auggccguuc ugggcgauac cgcuugggac uucggguccg ugggaggcgu cuuuaacucu     1920

cucgggaagg gcauccacca gaucuucgga gccgcuuuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauucu gaucggaaca cuucucgugu ggcugggccu gaacacgaaa     2040

aaugggagca uuucccucac cugucuggcc cuuggaggcg uuaugaucuu ccugagcaca     2100

gccgugucug cugaugucgg gugcuccgug gacuuuagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4400
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 1 opt

<400>  4400
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4401
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  4401
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4402
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 1 opt

<400>  4402
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccguug ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa cgcuaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu gagcaccgcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4403
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 1 opt

<400>  4403
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucggguacg agacggacga agauagagcc aagguggagg ucacaccuaa cagcccgcgg     1080

gccgaggcua cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga     1140

cuggauuuuu cugaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag     1200

gagugguucc acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau     1260

uggaauaaca aagaagcucu ggucgaguuu aaggaugccc acgccaaacg ccagacggug     1320

guugugcucg gaagccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggacg gcgccaaggg acggcuguuc uccggccacc ucaagugcag acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucucucugua cagcugccuu uaccuucaca     1500

aagguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gacgggccgu gcaaaauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacggcuca uuaccgccaa uccugugauc acggaaucca cagagaacag caagaugaug     1680

cuggaacuug auccucccuu uggcgacucu uacaucguga uuggggucgg cgauaagaaa     1740

aucacccacc acuggcauag auccggaagc acaauuggca aggcuuucga ggccaccgug     1800

cgcggggcua aacggauggc cguucuggga gacaccgcgu gggauuucgg cuccgucggg     1860

ggaguguuua auucucucgg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc     1920

cuguucggag gcauguccug guuuucucaa auccugaucg ggacacuucu cguguggcug     1980

ggacugaaca cgaagaacgg cagcauuucc cucaccuguc uggcccuugg gggaguuaug     2040

aucuuccuga gcacagcugu gucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4404
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 1 opt

<400>  4404
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4405
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 opt

<400>  4405
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggauau uguccuugaa cacgggggau gugugacagu uauggcccaa      180

gacaaaccua cgguggauau cgagcugguc accacaaccg uguccaauau ggcggaaguu      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu gcaugggucu cagcacuccg gaaugauugu caacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guugaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu ggaguucaag gaugcucacg ccaagcggca gacagucgug      840

guucucggga gccaggaagg agcggugcau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auccccgcgg agacccuuca cgggacagug accguugaag ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauua cagccaaucc cgugaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucguuauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4406
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 opt

<400>  4406
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4407
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 opt

<400>  4407
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgg cuaugaaaca      540

gaugaggacc gggccaaggu ugaagugacc ccuaacagcc cccgcgcuga ggccacacuc      600

gggggauuug gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau      660

cuguacuacc ucacgaugaa uaacaaacau uggcuggugc acaaggaaug guuucaugac      720

auuccccuuc cuuggcacgc gggagccgac acaggcaccc cgcacuggaa uaacaaagag      780

gcucuggucg aguucaagga ugcccaugcu aagcggcaga cagugguugu gcucgggagc      840

caggaaggag ccguccacac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu      900

aaaggacgcc ucuuuuccgg ccaucugaag uguagacuua aaauggauaa gcugcggcuc      960

aaaggcgugu cuuauagccu gugcaccgcc gccuucacau uuacgaaggu ucccgcugag     1020

acccuucacg ggacagugac cgucgaagug caauacgcgg gaacagacgg cccuuguaaa     1080

auccccgucc agauggccgu ggauaugcag acccugacgc cuguugggcg ccucauuaca     1140

gcuaaucccg ugaucaccga guccaccgaa aacagcaaga ugaugcugga gcuggacccg     1200

ccuuucggag auucuuauau ugucaucggc gugggggaca agaaaaucac acaccauugg     1260

cacagauccg gcagcaccau uggaaaggcc uuugaagcca caguucgggg cgcuaaacgc     1320

auggccgugc ucggggauac ggcgugggac uucggaucuu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4408
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 1 opt

<400>  4408
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4409
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       1 opt

<400>  4409
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcugag cggaaagggc      900

ucccugguga ccugcgccaa auucgccugc agcaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug uccguccacg gaagccaaca cucuggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccg     1080

aauagcccca gagcggaagc cacccuugga ggcuuuggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu cucugaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ucacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgagu ucaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaagccag gaaggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugucuu auucccucug uacagcugcc     1500

uuuaccuuca caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uuuggcgacu ccuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcagcggau ccacaauugg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuu     1860

ggcucugucg ggggagcucu caauagccug ggcaagggga uccaccagau uuucggcgcc     1920

gccuucaaau cccuguuugg aggcaugucu ugguucagcc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcuccauua gccugaugug ucuugcucuu     2040

gggggagugc ucaucuuucu gucuaccgcg guuuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4410
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 1
       opt

<400>  4410
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcugag cggaaagggc      900

ucccugguca caugcgccaa auucgccugc agcaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccg     1080

aauagcccca gagcggaagc cacccuugga ggcuuuggcu cccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu cucugaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ucacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggagu ucaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaagccag gaaggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucucuu auucccucug uaccgcugcc     1500

uuuacauuca ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagagag caccgaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uuuggcgacu ccuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcagcggau ccaccaucgg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuu     1860

ggcucuguug ggggagcucu caauagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuucaaau cccuguuugg aggcaugucu ugguucagcc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa cgcuaagaau ggcuccauca gccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuucu gucuaccgcc guguccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4411
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 1 
       opt

<400>  4411
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguc cggaaagggc      900

agccugguga ccugcgcuaa auucacaugc ucuaagaaga ugaccgggaa auccauccag      960

cccgagaauc ucgaauaucg cauuaugcug agcguucacg gaucucaaca uagcggcaug     1020

aucggguacg agacggacga agauagagcc aagguggagg ucacaccuaa cuccccgcgg     1080

gccgaggcua cccuuggagg cuuuggcucu cuggggcucg acugugaacc cagaaccgga     1140

cuggauuuca gcgaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag     1200

gagugguuuc acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau     1260

uggaauaaca aagaagcucu ggucgaguuc aaggaugccc acgccaaacg ccagacggug     1320

guugugcucg gaucccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggacg gcgccaaggg acggcuguuu agcggccacc ucaagugcag acugaaaaug     1440

gauaaacuuc gccugaaggg ggugucuuau ucccucugua cagcugccuu caccuuuaca     1500

aagguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gacgggccgu gcaaaauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacggcuca uuaccgccaa uccugugauc acggaaagca cagagaacuc uaagaugaug     1680

cuggaacuug auccucccuu cggcgacucc uacaucguga uuggggucgg cgauaagaaa     1740

aucacccacc acuggcauag aagcggaucc acaauuggca aggcuuuuga ggccaccgug     1800

cgcggggcua aacggauggc cguucuggga gacaccgcgu gggauuucgg cucugucggg     1860

ggaguguuca auagccucgg caaggggauc caccagauuu uuggcgccgc uuucaaaucc     1920

cuguuuggag gcaugucuug guucagccaa auccugaucg ggacacuucu cguguggcug     1980

ggacugaaca cgaagaacgg cuccauuagc cucaccuguc uggcccuugg gggaguuaug     2040

aucuuucugu cuacagcugu guccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4412
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 1 
       opt

<400>  4412
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcugag cggaaagggc      900

ucccugguga ccugcgccaa auucgccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccg     1080

aauagcccca gagcggaagc cacccuugga ggcuuuggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu cucugaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ucacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggagu ucaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaagccag gaaggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucucuu auucccucug uacagcugcc     1500

uuuaccuuca caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uuuggcgacu ccuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcagcggau ccacaaucgg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuu     1860

ggcucugucg ggggagcucu caauagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuucaaau cccuguuugg aggcaugucu ugguucagcc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcuccauca gccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuucu gucuaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4413
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       1 opt

<400>  4413
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugcagg acaccgggca ugaaacggau gagaaucggg cuaagguuga gaucacaccg     1080

aacucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucaa     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaga uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaucccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caacucucug ggcaagggga uccaccaaau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauccuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaagaac ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4414
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 1
       opt

<400>  4414
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguucagg acacggggca ugaaacagau gagaaucggg cuaaggugga gauuaccccg     1080

aacucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcaa     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaga uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaucccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccguug ggggagcucu caacucucug ggcaagggga uucaccaaau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa ugcuaagaac ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu gagcaccgcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4415
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 1 
       opt

<400>  4415
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguucagg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcaa     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaga uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaucccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caacucucug ggcaagggga uucaccaaau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauucuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaagaac ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4416
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4416
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacacuggg caaggccuuu     1800

uccaccaccc ucaaaggggc gcagcggcug gccgcucugg gagauacagc cugggacuuc     1860

ggcucuauug ggggaguguu caauagcauc ggcagagcug ugcaccaagu cuuugggggc     1920

gccuuccgca cgcuuuuugg aggcaugucc uggauuaccc aggggcucau gggagcccug     1980

cugcucugga ugggcgugaa cgcucgggau agaucuaucg cgcuggccuu ccuugcuaca     2040

gggggaguuc ugguguuucu cgccaccaau guccaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4417
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4417
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcacccuggg caaggccuuu     1800

uccaccacac ucaaaggggc gcagcggcug gccgcucugg gagauacggc cugggacuuc     1860

ggcucuaucg ggggaguguu caauagcauu ggcagagcug uccaccaagu guuugggggc     1920

gccuuccgca cccuuuuugg aggcaugucc uggaucacac aggggcucau gggagcccug     1980

cugcucugga ugggcguuaa cgcucgggau agaucuauug cgcuggccuu ccuugcuacc     2040

gggggagugc uggucuuucu cgccacaaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4418
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 1 opt

<400>  4418
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucggguacg agacggacga agauagagcc aagguggagg ucacaccuaa cagcccgcgg     1080

gccgaggcua cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga     1140

cuggauuuuu cugaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag     1200

gagugguucc acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau     1260

uggaauaaca aagaagcucu ggucgaguuu aaggaugccc acgccaaacg ccagacggug     1320

guugugcucg gaagccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggacg gcgccaaggg acggcuguuc uccggccacc ucaagugcag acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucucucugua cagcugccuu uaccuucaca     1500

aagguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gacgggccgu gcaaaauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacggcuca uuaccgccaa uccugugauc acggaaucca cagagaacag caagaugaug     1680

cuggaacuug auccucccuu uggcgacucu uacaucguga uuggggucgg cgauaagaaa     1740

aucacccacc acuggcauag auccggaagc acacugggca aggcuuucuc caccacccuc     1800

aaaggggccc agcgccuggc ugcccuggga gacacagcgu gggauuucgg cucuauuggg     1860

ggaguguuua auagcaucgg ccgggccguu caccaagugu ucgggggcgc uuuuagaacg     1920

cuuuucggag gcauguccug gauuacccag gggcucaugg gagcccugcu gcucuggaug     1980

ggcgucaacg cucgcgaccg gucuaucgcc cuggccuuuc uugcuacagg gggagugcug     2040

guuuuccucg cgaccaacgu gcaugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4419
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4419
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacacuggg caaggccuuu     1800

uccaccaccc ucaaaggggc gcagcggcug gccgcucugg gagauacagc cugggacuuc     1860

ggcucuaucg ggggaguguu caauagcauu ggcagagcug uccaccaagu guuugggggc     1920

gccuuccgca cgcuuuuugg aggcaugucc uggaucaccc aggggcucau gggagcccug     1980

cugcucugga ugggcguuaa cgcucgggau agaucuauug cgcuggccuu ccuugcuaca     2040

gggggagugc uggucuuucu cgccaccaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4420
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  4420
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4421
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4421
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagcggcg cggcgcugac       60

accagcgugg ggaucgucgg acugcuccug acaacggcca uggcggcuga aguuaccaga      120

cggggcuccg ccuacuauau guaccuugau cgcaaugacg ccggggaggc uauuucuuuc      180

cccacaaccc ugggaaugaa caaaugcuau auccagauua uggaucucgg ccacaugugu      240

gacgccacaa ugagcuacga augcccuaug cuggaugagg ggguggaacc ggacgaugug      300

gacuguuggu gcaauaccac auccaccugg gucguguaug gaacguguca ucacaagaaa      360

ggcgaggcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      420

caaacccgcu cucagacaug gcucgagagc agagaauaca ccaagcaccu gauccgggug      480

gagaacugga ucuuucgcaa uccuggcuuc gcgcuggccg cugccgcuau ugccuggcuc      540

cuggggucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcgccc      600

gccuacucca uuagaugcau cggaguuagc aaccgggauu uuguggaagg caugucuggg      660

ggaacauggg ucgacauugu gcuggagcau ggcgggugug ugaccgucau ggcucaggau      720

aagccuacag uugacaucga gcuugugacc accacagugu ccaauauggc cgaaguccgc      780

agcuacugcu augaggcuuc uaucuccgau auggccagcg acuccagaug ucccacgcaa      840

ggagaagccu accuggacaa acagucugau acccaguaug ugugcaagcg gacacucguu      900

gacagaggcu ggggcaacgg guguggacug uucggcaaag ggagccuggu gaccugcgcu      960

aaguuugcgu gcuccaagaa aaugacagga aagagcauuc agccugagaa ucucgaauac     1020

cgcaucaugc ugucugucca cggcucccaa cacagcggga ugauugugaa cgauaccgga     1080

caugagacgg acgagaaucg ggccaaaguu gaaaucacac cgaacucucc cagagcugag     1140

gccacccuug gcggcuucgg gagccuggga cucgauugug aaccucgcac cggccuggac     1200

uuuuccgauc uuuauuaccu gacaaugaau aacaagcacu ggcucgugca uaaagagugg     1260

uuccacgaca uuccccugcc uuggcacgcc ggggcugaua ccggaacacc ccauuggaau     1320

aacaaggaag cccuggucga guuuaaagac gcgcacgcua agcggcagac gguggucgug     1380

cucggcucuc aggagggggc cguucauacc gcccuggcug gcgcccuuga agcugagaug     1440

gauggagcca agggcagacu gagcuccggg caccucaaau gccgccugaa gauggacaaa     1500

cuucggcuga agggagugag cuauucucuc uguacagcgg ccuucaccuu uacaaaaauc     1560

ccggcugaaa cccugcaugg cacggucacc guggagguuc aguacgccgg gacagacgga     1620

ccuugcaagg ugcccgcuca aauggccguc gauaugcaga cccugacacc ugugggcaga     1680

cucaucaccg cgaaccccgu gauuacggaa uccacagaga auagcaagau gaugcuggag     1740

cuugaucccc cuuucgggga cucuuacauc guuauuggcg ucggagagaa aaagaucacc     1800

caccacuggc aucgcuccgg cagcacaauu gggaaagccu uugaagcuac cgugcgggga     1860

gccaagagaa uggcugugcu gggcgacacc gccugggauu ucggguccgu cggaggcgcc     1920

cucaacucuc uggggaaagg cauccaccag aucuucggag cugcguuuaa gagccuguuc     1980

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacac uccugaugug gcugggccuc     2040

aauacgaaga acgggagcau uucccugaug ugucuugccc ugggaggcgu gcucaucuuc     2100

cugagcaccg cuguuucugc cgacgugggg ugcuccgucg auuuuagcaa augaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  4422
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4422
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agcggcgcgg cgcugacacc       60

agcgugggga uugucggacu gcuccugaca acggccaugg cggcugaagu uaccagacgg      120

ggcuccgccu acuauaugua ccuugaucgc aaugacgccg gggaggcuau cucuuucccc      180

acaacccugg gaaugaacaa augcuauauu cagaucaugg aucucggcca caugugugac      240

gccacaauga gcuacgaaug cccuaugcug gaugaggggg uggaaccgga cgauguggac      300

uguuggugca auaccacauc caccuggguc guguauggaa cgugucauca caagaaaggc      360

gaggcuagac ggucucgcag agccguuacc cuucccagcc auuccacacg gaagcugcaa      420

acccgcucuc agacauggcu cgagagcaga gaauacacca agcaccugau ucggguggag      480

aacuggaucu uucgcaaucc uggcuucgcg cuggccgcug ccgcuaucgc cuggcuccug      540

ggguccagca cgucucagaa agucauuuau cuugugauga uccugcucau ugcgcccgcc      600

uacuccauca gaugcauugg aguuagcaac cgggauuuug uggaaggcau gucuggggga      660

acaugggucg acaucgugcu ggagcauggc ggguguguga ccgucauggc ucaggauaag      720

ccuacaguug acauugagcu ugugaccacc acagugucca auauggccga aguccgcagc      780

uacugcuaug aggcuucuau cuccgauaug gccagcgacu ccagaugucc cacgcaagga      840

gaagccuacc uggacaaaca gucugauacc caguaugugu gcaagcggac acucguugac      900

agaggcuggg gcaacgggug uggacuguuc ggcaaaggga gccuggugac cugcgcuaag      960

uuugcgugcu ccaagaaaau gacaggaaag agcauccagc cugagaaucu cgaauaccgc     1020

auuaugcugu cuguccacgg cucccaacac agcgggauga ucgugaacga uaccggacau     1080

gagacggacg agaaucgggc caaaguugaa auuacaccga acucucccag agcugaggcc     1140

acccuuggcg gcuucgggag ccugggacuc gauugugaac cucgcaccgg ccuggacuuu     1200

uccgaucuuu auuaccugac aaugaauaac aagcacuggc ucgugcauaa agagugguuc     1260

cacgacaucc cccugccuug gcacgccggg gcugauaccg gaacacccca uuggaauaac     1320

aaggaagccc uggucgaguu uaaagacgcg cacgcuaagc ggcagacggu ggucgugcuc     1380

ggcucucagg agggggccgu ucauaccgcc cuggcuggcg cccuugaagc ugagauggau     1440

ggagccaagg gcagacugag cuccgggcac cucaaaugcc gccugaagau ggacaaacuu     1500

cggcugaagg gagugagcua uucucucugu acagcggccu ucaccuuuac aaaaauuccg     1560

gcugaaaccc ugcauggcac ggucaccgug gagguucagu acgccgggac agacggaccu     1620

ugcaaggugc ccgcucaaau ggccgucgau augcagaccc ugacaccugu gggcagacuc     1680

aucaccgcga accccgugau cacggaaucc acagagaaua gcaagaugau gcuggagcuu     1740

gaucccccuu ucggggacuc uuacauuguu aucggcgucg gagagaaaaa gauuacccac     1800

cacuggcauc gcuccggcag cacaaucggg aaagccuuug aagcuaccgu gcggggagcc     1860

aagagaaugg cugugcuggg cgacaccgcc ugggauuucg gguccgucgg aggcgcccuc     1920

aacucucugg ggaaaggcau ucaccagauc uucggagcug cguuuaagag ccuguucggc     1980

gggauguccu gguuuucuca aauccuuauu ggaacacucc ugauguggcu gggccucaau     2040

acgaagaacg ggagcaucuc ccugaugugu cuugcccugg gaggcgugcu cauuuuccug     2100

agcaccgcug uuucugccga cguggggugc uccgucgauu uuagcaaaug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  4423
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4423
gggagaaagc uuaccaugaa cgccagaaag gagaaaaagc ggcgcggcgc ugacaccagc       60

guggggaucg ucggacugcu ccugacaacg gccauggcgg cugaaguuac cagacggggc      120

uccgccuacu auauguaccu ugaucgcaau gacgccgggg aggcuauuuc uuuccccaca      180

acccugggaa ugaacaaaug cuauauccag auuauggauc ucggccacau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggggugg aaccggacga uguggacugu      300

uggugcaaua ccacauccac cugggucgug uauggaacgu gucaucacaa gaaaggcgag      360

gcuagacggu cucgcagagc cguuacccuu cccagccauu ccacacggaa gcugcaaacc      420

cgcucucaga cauggcucga gagcagagaa uacaccaagc accugauccg gguggagaac      480

uggauuuuuc gcaauccugg cuucgcgcug gccgcugccg cuaucgccug gcuccugggg      540

uccagcacgu cucagaaagu caucuaucuu gugaugauuc ugcucaucgc gcccgccuac      600

uccauuagau gcaucggagu uagcaaccgg gauuuugugg aaggcauguc ugggggaaca      660

ugggucgaca uugugcugga gcauggcggg ugugugaccg ucauggcuca ggauaagccu      720

acaguugaca ucgagcuugu gaccaccaca guguccaaua uggccgaagu ccgcagcuac      780

ugcuaugagg cuucuauuuc cgauauggcc agcgacucca gaugucccac gcaaggagaa      840

gccuaccugg acaaacaguc ugauacccag uaugugugca agcggacacu cguugacaga      900

ggcuggggca acgggugugg acuguucggc aaagggagcc uggugaccug cgcuaaguuu      960

gcgugcucca agaaaaugac aggaaagagc auccagccug agaaucucga auaccgcauc     1020

augcugucug uccacggcuc ccaacacagc gggaugauug ugaacgauac cggacaugag     1080

acggacgaga aucgggccaa aguugaaauc acaccgaacu cucccagagc ugaggccacc     1140

cuuggcggcu ucgggagccu gggacucgau ugugaaccuc gcaccggccu ggacuuuucc     1200

gaucuuuauu accugacaau gaauaacaag cacuggcucg ugcauaaaga gugguuccac     1260

gacauucccc ugccuuggca cgccggggcu gauaccggaa caccccauug gaauaacaag     1320

gaagcccugg ucgaguuuaa agacgcgcac gcuaagcggc agacgguggu cgugcucggc     1380

ucucaggagg gggccguuca uaccgcccug gcuggcgccc uugaagcuga gauggaugga     1440

gccaagggca gacugagcuc cgggcaccuc aaaugccgcc ugaagaugga caaacuucgg     1500

cugaagggag ugagcuauuc ucucuguaca gcggccuuca ccuuuacaaa aaucccggcu     1560

gaaacccugc auggcacggu caccguggag guucaguacg ccgggacaga cggaccuugc     1620

aaggugcccg cucaaauggc cgucgauaug cagacccuga caccuguggg cagacucauu     1680

accgcgaacc ccgugaucac ggaauccaca gagaauagca agaugaugcu ggagcuugau     1740

cccccuuucg gggacucuua caucguuauu ggcgucggag agaaaaagau cacccaccac     1800

uggcaucgcu ccggcagcac aauugggaaa gccuuugaag cuaccgugcg gggagccaag     1860

agaauggcug ugcugggcga caccgccugg gauuucgggu ccgucggagg cgcccucaac     1920

ucucugggga aaggcaucca ccagauuuuc ggagcugcgu uuaagagccu guucggcggg     1980

auguccuggu uuucucaaau ccuuaucgga acacuccuga uguggcuggg ccucaauacg     2040

aagaacggga gcauuucccu gaugugucuu gcccugggag gcgugcucau cuuccugagc     2100

accgcuguuu cugccgacgu ggggugcucc gucgauuuua gcaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4424
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4424
gggagaaagc uuaccauggc cagaaaggag aaaaagcggc gcggcgcuga caccagcgug       60

gggaucgucg gacugcuccu gacaacggcc auggcggcug aaguuaccag acggggcucc      120

gccuacuaua uguaccuuga ucgcaacgac gccggggagg cuauuucuuu ccccacaacc      180

cugggaauga auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca      240

augagcuacg aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg      300

ugcaacacca cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu      360

agacggucuc gcagagccgu uacccuuccc agccauucca cacggaagcu gcaaacccgc      420

ucucagacau ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaauugg      480

auuuuucgca acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc      540

agcacgucuc agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc      600

auuagaugca ucggaguuag caaucgggau uuuguggaag gcaugucugg gggaacaugg      660

gucgacauug ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca      720

guugacaucg agcuugugac caccacagug uccaacaugg ccgaaguccg cagcuacugc      780

uaugaggcuu cuauuuccga uauggccagc gacuccagau gucccacgca aggagaagcc      840

uaccuggaca aacagucuga uacccaguau gugugcaagc ggacacucgu ugacagaggc      900

uggggcaaug gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg      960

ugcuccaaga aaaugacagg aaagagcauc cagccugaga accucgaaua ccgcaucaug     1020

cugucugucc acggcuccca acacagcggg augauuguga augauaccgg acaugagacg     1080

gacgagaacc gggccaaagu ugaaaucaca cccaauucuc ccagagcuga ggccacccuu     1140

ggcggcuucg ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau     1200

cuuuauuacc ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac     1260

auuccccugc cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa     1320

gcccuggucg aguuuaaaga cgcgcacgcu aagcggcaga cgguggucgu gcucggcucu     1380

caggaggggg ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc     1440

aagggcagac ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa     1560

acccugcaug gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag     1620

gugcccgcuc aaauggccgu cgauaugcag acccugacac cugugggcag acucauuacc     1680

gcgaaccccg ugaucacgga auccacagag aacagcaaga ugaugcugga gcuugacccg     1740

ccuuucgggg auucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaagcc uuugaagcua ccgugcgggg agccaagaga     1860

auggcugugc ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaauucu     1920

cuggggaaag gcauccacca gauuuucgga gcugcguuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag     2040

aaugggagca uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc     2100

gcuguuucug ccgacguggg gugcuccguc gauuuuagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4425
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4425
gggagaaagc uuaccaugag aaaggagaaa aagcggcgcg gcgccgacac cagcgugggg       60

aucgucggac ugcuccugac aacggcuaug gccgcggaag uuaccagacg gggcuccgcu      120

uacuauaugu accuugaucg caacgacgcc ggggaggcca uuucuuuccc cacaacccug      180

ggaaugaaua aaugcuauau ccagauuaug gaucucggcc acauguguga cgcuacaaug      240

agcuacgaau gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc      300

aacaccacau ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggccaga      360

cggucucgca gagcuguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu      420

cagacauggc ucgagagcag agaauacacc aagcaccuga uccgggugga gaauuggauu      480

uuucgcaacc cuggcuucgc ccuggcggcc gcugccaucg cuuggcuccu gggguccagc      540

acgucucaga aagucaucua ucuugugaug auucugcuca ucgcccccgc guacuccauu      600

agaugcaucg gaguuagcaa ucgggauuuu guggaaggca ugucuggggg aacauggguc      660

gacauugugc uggagcaugg cgggugugug accgucaugg cccaggauaa gccuacaguu      720

gacaucgagc uugugaccac cacagugucc aacauggcug aaguccgcag cuacugcuau      780

gaggccucua uuuccgauau ggcuagcgac uccagauguc ccacgcaagg agaagccuac      840

cuggacaaac agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg      900

ggcaaugggu guggacuguu cggcaaaggg agccugguga ccugcgccaa guuugcuugc      960

uccaagaaaa ugacaggaaa gagcauccag ccugagaacc ucgaauaccg caucaugcug     1020

ucuguccacg gcucccaaca cagcgggaug auugugaaug auaccggaca ugagacggac     1080

gagaaccggg cgaaaguuga aaucacaccc aauucuccca gagccgaggc uacccuuggc     1140

ggcuucggga gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu     1200

uauuaccuga caaugaacaa uaagcacugg cucgugcaua aagagugguu ccacgacauu     1260

ccccugccuu ggcacgccgg ggccgauacc ggaacacccc auuggaacaa uaaggaagcu     1320

cuggucgagu uuaaagacgc ccacgcgaag cggcagacgg uggucgugcu cggcucucag     1380

gagggggcug uucauaccgc ccuggccggc gcucuugaag ccgagaugga uggagcuaag     1440

ggcagacuga gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag     1500

ggagugagcu auucucucug uacagccgcg uucaccuuua caaaaauccc ggccgaaacc     1560

cugcauggca cggucaccgu ggagguucag uacgcuggga cagacggacc uugcaaggug     1620

cccgcccaaa uggcugucga uaugcagacc cugacaccug ugggcagacu cauuaccgcc     1680

aaccccguga ucacggaauc cacagagaac agcaagauga ugcuggagcu ugacccgccu     1740

uucggggauu cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau     1800

cgcuccggca gcacaauugg gaaagcguuu gaagccaccg ugcggggagc uaagagaaug     1860

gccgugcugg gcgacaccgc uugggauuuc ggguccgucg gaggcgcccu caauucucug     1920

gggaaaggca uccaccagau uuucggagcc gcuuuuaaga gccuguucgg cgggaugucc     1980

ugguuuucuc aaauccuuau cggaacacuc cugauguggc ugggccucaa cacgaagaau     2040

gggagcauuu cccugaugug ucuugcgcug ggaggcgugc ucaucuuccu gagcaccgcc     2100

guuucugcug acguggggug cuccgucgau uuuagcaaau gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  4426
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4426
gggagaaagc uuaccaugaa ggagaaaaag agacggggcg ccgacaccag cguggggauc       60

gucggacugc uccugacaac ggcuauggcc gcggaaguua cccgcagagg cuccgcuuac      120

uauauguacc uugaucggaa cgacgccggg gaggccauuu cuuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcccgcaga      360

ucucggcgcg cuguuacccu ucccagccau uccacaagaa agcugcaaac ccggucucag      420

acauggcucg agagccgcga auacaccaag caccugauca gaguggagaa uuggauuuuu      480

cggaacccug gcuucgcccu ggcggccgcu gccaucgcuu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cuccauucgc      600

ugcaucggag uuagcaauag agauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggccc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaac auggcugaag uccggagcua cugcuaugag      780

gccucuauuu ccgauauggc uagcgacucc cgcuguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg gggcuggggc      900

aaugggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaccucg aauacagaau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaaugaua ccggacauga gacggacgag     1080

aaccgcgcga aaguugaaau cacacccaau ucuccccggg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu agaaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagcgc cagacggugg ucgugcucgg cucucaggag     1380

ggggcuguuc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cggcugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccggcucau uaccgccaac     1680

cccgugauca cggaauccac agagaacagc aagaugaugc uggagcuuga cccgccuuuc     1740

ggggauucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcauaga     1800

uccggcagca caauugggaa agcguuugaa gccaccgugc gcggagcuaa gcggauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccgucggag gcgcccucaa uucucugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugcugacg uggggugcuc cgucgauuuu agcaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4427
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4427
gggagaaagc uuaccaugga gaagaaaaga cggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc uauggccgcg gaaguuaccc gcagaggcuc cgcuuacuau      120

auguaccuug aucggaacga cgccggggag gccauuucuu uccccacaac ccugggaaug      180

aauaagugcu auauccagau uauggaucuc ggccacaugu gugacgcuac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaacacc      300

acauccaccu gggucgugua uggaacgugu caucacaaaa agggcgaggc ccgcagaucu      360

cggcgcgcug uuacccuucc cagccauucc acaagaaaac ugcaaacccg gucucagaca      420

uggcucgaga gccgcgaaua caccaagcac cugaucagag uggagaauug gauuuuucgg      480

aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc uccugggguc cagcacgucu      540

cagaagguca ucuaucuugu gaugauucug cucaucgccc ccgcguacuc cauucgcugc      600

aucggaguua gcaauagaga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcccagg auaaaccuac aguugacauc      720

gagcuuguga ccaccacagu guccaacaug gcugaagucc ggagcuacug cuaugaggcc      780

ucuauuuccg auauggcuag cgacucccgc ugucccacgc aaggagaagc cuaccuggac      840

aagcagucug auacccagua ugugugcaaa agaacacucg uugaccgggg cuggggcaau      900

ggguguggac uguucggcaa ggggagccug gugaccugcg ccaaauuugc uugcuccaag      960

aagaugacag gaaaaagcau ccagccugag aaccucgaau acagaaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aaugauaccg gacaugagac ggacgagaac     1080

cgcgcgaagg uugaaaucac acccaauucu ccccgggccg aggcuacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccuaga accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga acaauaaaca cuggcucgug cauaaggagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggccga uaccggaaca ccccauugga acaauaaaga agcucugguc     1320

gaguuuaagg acgcccacgc gaaacgccag acgguggucg ugcucggcuc ucaggagggg     1380

gcuguucaua ccgcccuggc cggcgcucuu gaagccgaga uggauggagc uaagggccgg     1440

cugagcuccg ggcaccucaa gugcagacug aaaauggaca aacuucgccu gaagggagug     1500

agcuauucuc ucuguacagc cgcguucacc uuuacaaaga ucccggccga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcu gggacagacg gaccuugcaa agugcccgcc     1620

caaauggcug ucgauaugca gacccugaca ccugugggcc ggcucauuac cgccaacccc     1680

gugaucacgg aauccacaga gaacagcaag augaugcugg agcuugaccc gccuuucggg     1740

gauucuuaca ucguuauugg cgucggagaa aagaaaauca cccaccacug gcauagaucc     1800

ggcagcacaa uugggaaggc guuugaggcc accgugcgcg gagcuaaacg gauggccgug     1860

cugggcgaca ccgcuuggga uuucgggucc gucggaggcg cccucaauuc ucuggggaag     1920

ggcauccacc agauuuucgg agccgcuuuu aaaagccugu ucggcgggau guccugguuu     1980

ucucaaaucc uuaucggaac acuccugaug uggcugggcc ucaacacgaa gaaugggagc     2040

auuucccuga ugugucuugc gcugggaggc gugcucaucu uccugagcac cgccguuucu     2100

gcugacgugg ggugcuccgu cgauuuuagc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  4428
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4428
gggagaaagc uuaccaugaa gaaaagacgg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuga caacggcuau ggccgcggag guuacccgca gaggcuccgc uuacuauaug      120

uaccuugauc ggaacgacgc cggggaagcc auuucuuucc ccacaacccu gggaaugaau      180

aagugcuaua uccagauuau ggaucucggc cacaugugug acgcuacaau gagcuacgag      240

ugcccuaugc uggaugaagg gguggagccg gacgaugugg acuguuggug caacaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaaaaagg gcgaagcccg cagaucucgg      360

cgcgcuguua cccuucccag ccauuccaca agaaaacugc aaacccgguc ucagacaugg      420

cucgagagcc gcgaguacac caagcaccug aucagagugg aaaauuggau uuuucggaac      480

ccuggcuucg cccuggcggc cgcugccauc gcuuggcucc ugggguccag cacgucucag      540

aaggucaucu aucuugugau gauucugcuc aucgcccccg cguacuccau ucgcugcauc      600

ggaguuagca auagagauuu uguggagggc augucugggg gaacaugggu cgacauugug      660

cuggaacaug gcgggugugu gaccgucaug gcccaggaua aaccuacagu ugacaucgag      720

cuugugacca ccacaguguc caacauggcu gagguccgga gcuacugcua ugaagccucu      780

auuuccgaua uggcuagcga cucccgcugu cccacgcaag gagaggccua ccuggacaag      840

cagucugaua cccaguaugu gugcaaaaga acacucguug accggggcug gggcaauggg      900

uguggacugu ucggcaaggg gagccuggug accugcgcca aauuugcuug cuccaagaag      960

augacaggaa aaagcaucca gccugaaaac cucgaguaca gaaucaugcu gucuguccac     1020

ggcucccaac acagcgggau gauugugaau gauaccggac augaaacgga cgagaaccgc     1080

gcgaagguug agaucacacc caauucuccc cgggccgaag cuacccuugg cggcuucggg     1140

agccugggac ucgauuguga gccuagaacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaca auaaacacug gcucgugcau aaggaauggu uccacgacau uccccugccu     1260

uggcacgccg gggccgauac cggaacaccc cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggacg cccacgcgaa acgccagacg guggucgugc ucggcucuca ggagggggcu     1380

guucauaccg cccuggccgg cgcucuugag gccgaaaugg auggagcuaa gggccggcug     1440

agcuccgggc accucaagug cagacugaaa auggacaaac uucgccugaa gggagugagc     1500

uauucucucu guacagccgc guucaccuuu acaaagaucc cggccgagac ccugcauggc     1560

acggucaccg uggaaguuca guacgcuggg acagacggac cuugcaaagu gcccgcccaa     1620

auggcugucg auaugcagac ccugacaccu gugggccggc ucauuaccgc caaccccgug     1680

aucacggagu ccacagaaaa cagcaagaug augcuggagc uugacccgcc uuucggggau     1740

ucuuacaucg uuauuggcgu cggagagaag aaaaucaccc accacuggca uagauccggc     1800

agcacaauug ggaaggcguu ugaagccacc gugcgcggag cuaaacggau ggccgugcug     1860

ggcgacaccg cuugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaagggc     1920

auccaccaga uuuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu     2040

ucccugaugu gucuugcgcu gggaggcgug cucaucuucc ugagcaccgc cguuucugcu     2100

gacguggggu gcuccgucga uuuuagcaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  4429
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4429
gggagaaagc uuaccaugaa gagacggggc gccgacacca gcguggggau cgucggacug       60

cuccugacaa cggcuauggc cgcggagguu acccgcagag gcuccgcuua cuauauguac      120

cuugaucgga acgacgccgg ggaagccauu ucuuucccca caacccuggg aaugaauaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg cuacaaugag cuacgagugc      240

ccuaugcugg augaaggggu ggagccggac gauguggacu guuggugcaa caccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aagcccgcag aucucggcgc      360

gcuguuaccc uucccagcca uuccacaaga aagcugcaaa cccggucuca gacauggcuc      420

gagagccgcg aguacaccaa acaccugauc agaguggaaa auuggauuuu ucggaacccu      480

ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaag      540

gucaucuauc uugugaugau ucugcucauc gcccccgcgu acuccauucg cugcaucgga      600

guuagcaaua gagauuuugu ggagggcaug ucugggggaa caugggucga cauugugcug      660

gaacauggcg ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa cauggcugag guccggagcu acugcuauga agccucuauu      780

uccgauaugg cuagcgacuc ccgcuguccc acgcaaggag aggccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagagaaca cucguugacc ggggcugggg caaugggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caaaaagaug      960

acaggaaaga gcauccagcc ugaaaaccuc gaguacagaa ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaaugau accggacaug aaacggacga gaaccgcgcg     1080

aaaguugaga ucacacccaa uucuccccgg gccgaagcua cccuuggcgg cuucgggagc     1140

cugggacucg auugugagcc uagaaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaacaaua agcacuggcu cgugcauaaa gaaugguucc acgacauucc ccugccuugg     1260

cacgccgggg ccgauaccgg aacaccccau uggaacaaua aggaggcucu ggucgaauuu     1320

aaagacgccc acgcgaagcg ccagacggug gucgugcucg gcucucagga gggggcuguu     1380

cauaccgccc uggccggcgc ucuugaggcc gaaauggaug gagcuaaagg ccggcugagc     1440

uccgggcacc ucaagugcag acugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucucucugua cagccgcguu caccuuuaca aaaaucccgg ccgagacccu gcauggcacg     1560

gucaccgugg aaguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug     1620

gcugucgaua ugcagacccu gacaccugug ggccggcuca uuaccgccaa ccccgugauc     1680

acggagucca cagaaaacag caaaaugaug cuggagcuug acccgccuuu cggggauucu     1740

uacaucguua uuggcgucgg agagaagaag aucacccacc acuggcauag auccggcagc     1800

acaauuggga aagcguuuga agccaccgug cgcggagcua agcggauggc cgugcugggc     1860

gacaccgcuu gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaca cgaaaaaugg gagcauuucc     2040

cugauguguc uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac     2100

guggggugcu ccgucgauuu uagcaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4430
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4430
gggagaaagc uuaccaugag acggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg cuauggccgc ggagguuacc cgcagaggcu ccgcuuacua uauguaccuu      120

gaucggaacg acgccgggga agccauuucu uuccccacaa cccugggaau gaauaagugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcua caaugagcua cgagugcccu      240

augcuggaug aaggggugga gccggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaaa aagggcgaag cccgcagauc ucggcgcgcu      360

guuacccuuc ccagccauuc cacaagaaaa cugcaaaccc ggucucagac auggcucgag      420

agccgcgagu acaccaagca ccugaucaga guggaaaauu ggauuuuucg gaacccuggc      480

uucgcccugg cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu      600

agcaauagag auuuugugga gggcaugucu gggggaacau gggucgacau ugugcuggaa      660

cauggcgggu gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggcugagguc cggagcuacu gcuaugaagc cucuauuucc      780

gauauggcua gcgacucccg cugucccacg caaggagagg ccuaccugga caagcagucu      840

gauacccagu augugugcaa aagaacacuc guugaccggg gcuggggcaa ugggugugga      900

cuguucggca aggggagccu ggugaccugc gccaaauuug cuugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga aaaccucgag uacagaauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaaa cggacgagaa ccgcgcgaag     1080

guugagauca cacccaauuc uccccgggcc gaagcuaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugagccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccccu gccuuggcac     1260

gccggggccg auaccggaac accccauugg aacaauaaag aggcucuggu cgaauuuaag     1320

gacgcccacg cgaaacgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau     1380

accgcccugg ccggcgcucu ugaggccgaa auggauggag cuaagggccg gcugagcucc     1440

gggcaccuca aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu     1500

cucuguacag ccgcguucac cuuuacaaag aucccggccg agacccugca uggcacgguc     1560

accguggaag uucaguacgc ugggacagac ggaccuugca aagugcccgc ccaaauggcu     1620

gucgauaugc agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca     1800

auugggaagg cguuugaagc caccgugcgc ggagcuaaac ggauggccgu gcugggcgac     1860

accgcuuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa gggcauccac     1920

cagauuuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacgug     2100

gggugcuccg ucgauuuuag caaaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4431
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4431
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga gguuacccgg cgcggcuccg cuuacuauau guaccuugau      120

agaaacgacg ccggggaagc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga gugcccuaug      240

cuggaugaag ggguggagcc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaagccc ggcgcucuag acgggcuguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgagagc      420

cgggaguaca ccaagcaccu gauccgcgug gaaaauugga uuuuuagaaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaggg caugucuggg ggaacauggg ucgacauugu gcuggaacau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaggucaga agcuacugcu augaagccuc uauuuccgau      780

auggcuagcg acucccggug ucccacgcaa ggagaggccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugaaaa ccucgaguac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugaaacgg acgagaacag agcgaagguu     1080

gagaucacac ccaauucucc ccgcgccgaa gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug agccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggaaugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaagagg cucuggucga auuuaaggac     1320

gcccacgcga aaagacagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga ggccgaaaug gauggagcua agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaagauc ccggccgaga cccugcaugg cacggucacc     1560

guggaaguuc aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg ccaaccccgu gaucacggag     1680

uccacagaaa acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggcgu uugaagccac cgugagagga gcuaaacgca uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacgugggg     2100

ugcuccgucg auuuuagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4432
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4432
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaggu uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

aacgacgccg gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug      240

gaugaagggg uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caaaaagggc gaagccagac ggucucgcag agcuguuacc      360

cuucccagcc auuccacacg gaaacugcaa acccgcucuc agacauggcu cgagagcaga      420

gaguacacca agcaccugau ccggguggaa aauuggauuu uucgcaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauua gaugcaucgg aguuagcaau      600

cgggauuuug uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga gguccgcagc uacugcuaug aagccucuau uuccgauaug      780

gcuagcgacu ccagaugucc cacgcaagga gaggccuacc uggacaagca gucugauacc      840

caguaugugu gcaaacggac acucguugac cgcggcuggg gcaaugggug uggacuguuc      900

ggcaagggga gccuggugac cugcgccaaa uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gaaacggacg agaaccgggc gaagguugag     1080

aucacaccca auucucccag agccgaagcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugagc cucgcaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc     1320

cacgcgaaac ggcagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaggc cgaaauggau ggagcuaagg gcagacugag cuccgggcac     1440

cucaaaugcc gccugaagau ggacaaacuu cggcugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug     1560

gaaguucagu acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggcagacuc auuaccgcca accccgugau cacggagucc     1680

acagaaaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcauc gcuccggcag cacaauuggg     1800

aaggcguuug aagccaccgu gcggggagcu aaaagaaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu     1920

uucggagccg cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga cguggggugc     2100

uccgucgauu uuagcaaaug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4433
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4433
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccugacaacg       60

gcuauggccg cggagguuac cagacgggga uccgcuuacu auauguaccu ugaucgcaac      120

gacgccggcg aagccauuuc uuuccccaca acccugggga ugaauaagug cuauauccag      180

auuauggauc ucggacacau gugugacgcu acaaugagcu acgagugccc uaugcuggau      240

gaaggcgugg agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uaugggacgu gucaucacaa aaagggagaa gccagacggu cucgcagagc uguuacccuu      360

cccagccauu ccacacggaa acugcaaacc cgcucucaga cauggcucga gagcagagag      420

uacaccaagc accugauccg gguggaaaau uggauuuuuc gcaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggc uccagcacgu cucagaaagu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauuagau gcaucggggu uagcaaucgg      600

gauuuugugg agggaauguc uggcgggaca ugggucgaca uugugcugga acauggaggc      660

ugugugaccg ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaggu ccgcagcuac ugcuaugaag ccucuauuuc cgauauggcu      780

agcgacucca gaugucccac gcaaggggag gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aacggacacu cguugaccgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucca agaaaaugac aggaaagagc      960

auccagccug aaaaccucga guacagaauc augcugucug uccacggguc ccaacacagc     1020

ggcaugauug ugaaugauac cgggcaugaa acggacgaga accgggcgaa gguugagauc     1080

acacccaauu cucccagagc cgaagcuacc cuuggaggcu ucggcagccu ggggcucgau     1140

ugugagccuc gcaccggacu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga augguuccac gacauucccc ugccuuggca cgccggcgcc     1260

gauaccggga caccccauug gaacaauaaa gaggcucugg ucgaauuuaa ggacgcccac     1320

gcgaaacggc agacgguggu cgugcucgga ucucaggagg gcgcuguuca uaccgcccug     1380

gccggggcuc uugaggccga aauggauggc gcuaagggaa gacugagcuc cggccaccuc     1440

aaaugccgcc ugaagaugga caagcugcgg cugaaagggg ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gagacccugc auggaacggu caccguggaa     1560

guucaguacg cuggcacaga cgggccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg aagacucauu accgccaacc ccgugaucac ggaguccaca     1680

gaaaacagca agaugaugcu ggagcuugac ccgccuuucg gcgauucuua caucguuauu     1740

ggggucggcg agaaaaagau cacccaccac uggcaucgcu ccggaagcac aauuggcaag     1800

gcguuugaag ccaccgugag aggggcuaaa cggauggccg ugcugggaga caccgcuugg     1860

gauuucggcu ccgucggggg agcccucaau ucucugggca aggggaucca ccagauuuuc     1920

ggcgccgcuu uuaaaagccu guucggaggc auguccuggu uuucucaaau ccuuaucggg     1980

acacuccuga uguggcuggg acucaacacg aagaauggca gcauuucccu gaugugucuu     2040

gcgcuugggg gagugcucau cuuccugagc accgccguuu cugcugacgu gggcugcucc     2100

gucgauuuua gcaaaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4434
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4434
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu gacaacggcc       60

auggcugccg agguuaccag acggggaucc gcguacuaua uguaccuuga ucgcaacgac      120

gcuggcgaag ccauuucuuu ccccacaacc cuggggauga auaagugcua uauccagauu      180

auggaucucg gacacaugug ugacgccaca augagcuacg agugcccuau gcuggaugaa      240

ggcguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

gggacguguc aucacaaaaa gggagaagcu agacggucuc gcagagccgu uacccuuccc      360

agccauucca cacggaaacu gcaaacccgc ucucagacau ggcucgagag cagagaguac      420

accaagcacc ugauccgggu ggaaaauugg auuuuucgca acccuggcuu cgcucuggcc      480

gcggccgcua ucgccuggcu ccugggcucc agcacgucuc agaaagucau cuaucuugug      540

augauucugc ucaucgcucc cgccuacucc auuagaugca ucgggguuag caaucgggau      600

uuuguggagg gaaugucugg cgggacaugg gucgacauug ugcuggaaca uggaggcugu      660

gugaccguca uggcgcagga uaagccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg ccgagguccg cagcuacugc uaugaagcua gcauuuccga uauggccagc      780

gacuccagau gucccacgca aggggaggcu uaccuggaca agcagucuga uacccaguau      840

gugugcaaac ggacacucgu ugaccgcgga uggggcaaug gcugugggcu guucggaaag      900

ggcagccugg ugaccugcgc caaauuugcc ugcuccaaga aaaugacagg gaagucuauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggauccca acacagcggc     1020

augauuguga augauaccgg gcaugaaacg gacgagaacc gggcuaaggu ugagaucaca     1080

cccaauucuc ccagagcgga agccacccuu ggaggcuucg gcagccuggg gcucgauugu     1140

gagccucgca ccggacugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc uggcgccgau     1260

accgggacac cccauuggaa caauaaagag gcccuggucg aauuuaagga cgcucacgcc     1320

aaacggcaga cgguggucgu gcucggaucu caggagggcg cgguucauac cgcucuggcc     1380

ggggcccuug aggcugaaau ggauggcgcc aagggaagac ugagcuccgg ccaccucaaa     1440

ugccgccuga agauggacaa gcugcggcug aaagggguga gcuauucucu cuguacagcu     1500

gccuucaccu uuacaaagau cccggcggag acccugcaug gaacggucac cguggaaguu     1560

caguacgccg gcacagacgg gccuugcaaa gugcccgcuc aaauggccgu cgauaugcag     1620

acccugacac cugugggaag acucauuacc gcuaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucggcg auucuuacau cguuauuggg     1740

gucggcgaga aaaagaucac ccaccacugg caucgcuccg gaagcacaau uggcaaggcc     1800

uuugaagcga ccgugagagg ggccaaacgg auggcugugc ugggagacac cgccugggau     1860

uucggcuccg ucgggggagc ucucaauucu cugggcaagg ggauccacca gauuuucggc     1920

gccgccuuua aaagccuguu cggaggcaug uccugguuuu cucaaauccu uaucgggaca     1980

cuccugaugu ggcugggacu caacacgaag aauggcagca uuucccugau gugucuugcu     2040

cuugggggag ugcucaucuu ccugagcacc gcgguuucug ccgacguggg cugcuccguc     2100

gauuuuagca aaugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4435
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4435
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccg augugggcug cuccgucgac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4436
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4436
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgcgg uuaccagacg gggauccgcu uacuauaugu accuugaccg caacgaugcc      120

ggcgaggcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugcuacaaug agcuacgaau gcccuaugcu ggacgagggc      240

guggaaccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaggccaga cggucucgca gagcuguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgaaagcag agaguacacc      420

aagcaccuga uccgggugga gaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggaaggaa ugucuggcgg gacauggguc gauauugugc uggagcaugg aggcugugug      660

accgucaugg cccaggacaa gccuacaguu gauaucgaac uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaggccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaagccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauccag      960

ccugagaacc ucgaauacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugagacggau gaaaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaggc uacccuugga ggcuucggca gccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccccugccuu ggcacgccgg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaagcu cuggucgagu uuaaggaugc ccacgcgaaa     1320

cggcagacgg uggucgugcu cggaucucag gaaggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgagaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgaaacc cugcauggaa cggucaccgu ggagguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaggccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcug augugggcug cuccgucgac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4437
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4437
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcggc uguuacccgg cgcggcuccg ccuacuauau guaccuugau      120

agaaacgacg ccggggaggc uauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgccacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaggcuc ggcgcucuag acgggccguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgaaagc      420

cgggaguaca ccaagcaccu gauccgcgug gagaauugga uuuuuagaaa cccuggcuuc      480

gcgcuggccg cugccgcuau cgccuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgcgccc gccuacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcucaggau aagccuacag uugacaucga acuugugacc      720

accacagugu ccaacauggc cgaggucaga agcuacugcu augaggcuuc uauuuccgau      780

auggccagcg acucccggug ucccacgcaa ggagaagccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcu aaauuugcgu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgaaaacag agccaagguu     1080

gagaucacac ccaauucucc ccgcgcugag gccacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggcugaua ccggaacacc ccauuggaac aauaaagaag cccuggucga guuuaaggac     1320

gcgcacgcua aaagacagac gguggucgug cucggcucuc aggaaggggc cguucauacc     1380

gcccuggcug gcgcccuuga ggcugagaug gauggagcca agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagcgg ccuucaccuu uacaaagauc ccggcugaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgccgg gacagacgga ccuugcaaag ugcccgcuca aauggccguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg cgaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggaa cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggccu uugaggcuac cgugagagga gccaaacgca uggcugugcu gggcgacacc     1860

gccugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag cugcguuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugccc ugggaggcgu gcucaucuuc cugagcaccg cuguuucugc cgacgugggg     2100

ugcuccgucg auuuuagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4438
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4438
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gaggcguuac gcggcgcggg      120

agcgccuacu auauguaccu ggauagaaac gacgcuggag aggccauuuc cuuucccacc      180

acacuuggca ugaauaaaug cuauauccaa auuauggauc uggggcauau gugugacgcg      240

accaugucuu acgaaugccc uaugcucgau gagggagugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggcaccu gucaccauaa gaaaggcgag      360

gcucggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg guuugcccuu gcugccgcug ccaucgcgug gcugcucgga      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ccccgcuuac      600

ucuauucggu gcaucggcgu uuccaaucgc gauuucgugg aagggaugag cggaggcacg      660

ugggucgaca uugugcucga gcacggggga ugugugacag ucauggccca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggcugaggu cagauccuac      780

ugcuaugagg ccagcauuuc cgauauggcg ucugacagcc ggugucccac acagggcgaa      840

gccuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggga auggaugugg ccuguuuggg aaaggaagcc uugugacaug cgcuaaguuc      960

gccugcucua aaaagaugac cggcaagucc auccaaccug agaaccugga auaccggauc     1020

augcucagcg uccacggguc ucagcauagc ggaaugauug ugaaugauac aggccacgag     1080

accgacgaaa acagagcuaa aguugagauc acgcccaauu ccccgcgcgc cgaggccaca     1140

cugggcgggu uuggaucucu uggccuggau ugugaaccuc ggaccgggcu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcuggagcg gauacaggca caccccacug gaacaauaag     1320

gaagcccuug ucgaguucaa agacgcucau gccaagagac agacaguggu cgugcugggg     1380

ucccaggaag gcgccguuca cacggcucuc gccggagcgc uggaggcuga gauggauggc     1440

gccaaagggc gccuuagcuc uggacaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggcg uguccuauag ccucuguacc gccgcuuuua cauucaccaa aaucccggcc     1560

gaaacacugc acgggaccgu cacgguggag guucaauacg cuggaaccga cggcccuugc     1620

aaggugcccg cccagauggc ggucgauaug cagacacuua ccccuguggg gcgccugauu     1680

acagccaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gcgacagcua caucguuauu ggagucggcg agaagaagau cacacaccau     1800

uggcaccggu ccgggucuac cauuggaaag gcuuucgagg ccacagugag aggcgcuaaa     1860

cgcauggccg ugcuggggga caccgcgugg gauuucggaa gcgucggcgg ggcccuuaau     1920

ucccucggca aaggaaucca ucaaauuuuu ggcgcugccu ucaagucucu guuuggggga     1980

augagcuggu ucucccagau ccugaucggc acccuccuga uguggcuugg gcugaacacc     2040

aaaaauggaa gcauuucucu caugugucug gcucugggcg gggugcuuau cuuucucucc     2100

acggccguua gcgccgacgu gggcugcucu gucgauuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4439
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4439
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gagccguuac gcggcgcggc      120

agcgcuuacu auauguaccu ggauagaaac gacgccgggg aggcgauuuc cuuucccacc      180

acacuuggaa ugaauaaaug cuauauccaa auuauggauc ugggccauau gugugacgcu      240

accaugucuu acgaaugccc uaugcucgau gagggggugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggaaccu gucaccauaa gaaaggcgag      360

gcccggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg cuuugcucuu gccgcugccg cgaucgccug gcugcucggg      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ucccgccuac      600

ucuauucggu gcaucggagu uuccaaucgc gauuucgugg aaggcaugag cgggggaacg      660

ugggucgaca uugugcucga gcacggcggg ugugugacag ucauggcuca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggccgaggu cagauccuac      780

ugcuaugagg cgagcauuuc cgauauggcc ucugacagcc ggugucccac acagggagag      840

gcuuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggca augggugugg acuguuuggc aaagggagcc uugugacaug cgccaaguuc      960

gcuugcucua aaaagaugac cggaaagucc auccaaccug aaaaccugga auaccggauc     1020

augcucagcg uccacggcuc ucagcauagc gggaugauug ugaaugauac aggacacgag     1080

accgacgaaa acagagccaa aguugagauc acgcccaauu ccccgcgcgc cgaggcuaca     1140

cugggcggcu uugggucucu uggacuggau ugugaaccuc ggaccggccu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcgggggcc gauacaggaa caccccacug gaacaauaag     1320

gaagcucuug ucgaguucaa agacgcccau gccaagagac agacaguggu cgugcugggc     1380

ucccaggaag gggcuguuca cacggcccuc gcgggcgcuc uggaggccga gauggaugga     1440

gccaaaggcc gccuuagcuc ugggcaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggag uguccuauag ccucuguacc gcugccuuua cauucaccaa aaucccggcu     1560

gaaacacugc acggcaccgu cacgguggag guucaauacg ccgggaccga cggaccuugc     1620

aaggugcccg cgcagauggc cgucgauaug cagacacuua ccccuguggg ccgccugauu     1680

acagcuaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gggacagcua caucguuauu ggcgucggag agaagaagau cacacaccau     1800

uggcaccggu ccggcucuac cauugggaaa gccuucgagg cuacagugag aggagccaag     1860

cgcauggcgg ugcugggcga caccgccugg gauuucggga gcgucggagg cgcucuuaau     1920

ucccucggga aaggcaucca ucaaauuuuu ggagccgcuu ucaagucucu guuuggcggg     1980

augagcuggu ucucccagau ccugaucgga acccuccuga uguggcuugg ccugaacacc     2040

aaaaauggga gcauuucucu caugugucug gcccugggag gcgugcuuau cuuucucucc     2100

acggccguua gcgcugacgu ggggugcucu gucgauuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4440
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4440
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4441
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4441
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagcggcg cggcgcugac       60

accagcgugg ggaucgucgg acugcuccug acaacggcca uggcggcuga aguuaccaga      120

cggggcuccg ccuacuauau guaccuugau cgcaaugacg ccggggaggc uauuucuuuc      180

cccacaaccc ugggaaugaa caaaugcuau auccagauua uggaucucgg ccacaugugu      240

gacgccacaa ugagcuacga augcccuaug cuggaugagg ggguggaacc ggacgaugug      300

gacuguuggu gcaauaccac auccaccugg gucguguaug gaacguguca ucacaagaaa      360

ggcgaggcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      420

caaacccgcu cucagacaug gcucgagagc agagaauaca ccaagcaccu gauccgggug      480

gagaacugga ucuuucgcaa uccuggcuuc gcgcuggccg cugccgcuau ugccuggcuc      540

cuggggucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcgccc      600

gccuacucca uuagaugcau cggaguuagc aaccgggauu uuguggaagg caugucuggg      660

ggaacauggg ucgacauugu gcuggagcau ggcgggugug ugaccgucau ggcucaggau      720

aagccuacag uugacaucga gcuugugacc accacagugu ccaauauggc cgaaguccgc      780

agcuacugcu augaggcuuc uaucuccgau auggccagcg acuccagaug ucccacgcaa      840

ggagaagccu accuggacaa acagucugau acccaguaug ugugcaagcg gacacucguu      900

gacagaggcu ggggcaacgg guguggacug uucggcaaag ggagccuggu gaccugcgcu      960

aaguuugcgu gcuccaagaa aaugacagga aagagcauuc agccugagaa ucucgaauac     1020

cgcaucaugc ugucugucca cggcucccaa cacagcggga ugauugugaa cgauaccgga     1080

caugagacgg acgagaaucg ggccaaaguu gaaaucacac cgaacucucc cagagcugag     1140

gccacccuug gcggcuucgg gagccuggga cucgauugug aaccucgcac cggccuggac     1200

uuuuccgauc uuuauuaccu gacaaugaau aacaagcacu ggcucgugca uaaagagugg     1260

uuccacgaca uuccccugcc uuggcacgcc ggggcugaua ccggaacacc ccauuggaau     1320

aacaaggaag cccuggucga guuuaaagac gcgcacgcua agcggcagac gguggucgug     1380

cucggcucuc aggagggggc cguucauacc gcccuggcug gcgcccuuga agcugagaug     1440

gauggagcca agggcagacu gagcuccggg caccucaaau gccgccugaa gauggacaaa     1500

cuucggcuga agggagugag cuauucucuc uguacagcgg ccuucaccuu uacaaaaauc     1560

ccggcugaaa cccugcaugg cacggucacc guggagguuc aguacgccgg gacagacgga     1620

ccuugcaagg ugcccgcuca aauggccguc gauaugcaga cccugacacc ugugggcaga     1680

cucaucaccg cgaaccccgu gauuacggaa uccacagaga auagcaagau gaugcuggag     1740

cuugaucccc cuuucgggga cucuuacauc guuauuggcg ucggagagaa aaagaucacc     1800

caccacuggc aucgcuccgg cagcacaauu gggaaagccu uugaagcuac cgugcgggga     1860

gccaagagaa uggcugugcu gggcgacacc gccugggauu ucggguccgu cggaggcgcc     1920

cucaacucuc uggggaaagg cauccaccag aucuucggag cugcguuuaa gagccuguuc     1980

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacac uccugaugug gcugggccuc     2040

aauacgaaga acgggagcau uucccugaug ugucuugccc ugggaggcgu gcucaucuuc     2100

cugagcaccg cuguuucugc cgacgugggg ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  4442
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4442
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agcggcgcgg cgcugacacc       60

agcgugggga uugucggacu gcuccugaca acggccaugg cggcugaagu uaccagacgg      120

ggcuccgccu acuauaugua ccuugaucgc aaugacgccg gggaggcuau cucuuucccc      180

acaacccugg gaaugaacaa augcuauauu cagaucaugg aucucggcca caugugugac      240

gccacaauga gcuacgaaug cccuaugcug gaugaggggg uggaaccgga cgauguggac      300

uguuggugca auaccacauc caccuggguc guguauggaa cgugucauca caagaaaggc      360

gaggcuagac ggucucgcag agccguuacc cuucccagcc auuccacacg gaagcugcaa      420

acccgcucuc agacauggcu cgagagcaga gaauacacca agcaccugau ucggguggag      480

aacuggaucu uucgcaaucc uggcuucgcg cuggccgcug ccgcuaucgc cuggcuccug      540

ggguccagca cgucucagaa agucauuuau cuugugauga uccugcucau ugcgcccgcc      600

uacuccauca gaugcauugg aguuagcaac cgggauuuug uggaaggcau gucuggggga      660

acaugggucg acaucgugcu ggagcauggc ggguguguga ccgucauggc ucaggauaag      720

ccuacaguug acauugagcu ugugaccacc acagugucca auauggccga aguccgcagc      780

uacugcuaug aggcuucuau cuccgauaug gccagcgacu ccagaugucc cacgcaagga      840

gaagccuacc uggacaaaca gucugauacc caguaugugu gcaagcggac acucguugac      900

agaggcuggg gcaacgggug uggacuguuc ggcaaaggga gccuggugac cugcgcuaag      960

uuugcgugcu ccaagaaaau gacaggaaag agcauccagc cugagaaucu cgaauaccgc     1020

auuaugcugu cuguccacgg cucccaacac agcgggauga ucgugaacga uaccggacau     1080

gagacggacg agaaucgggc caaaguugaa auuacaccga acucucccag agcugaggcc     1140

acccuuggcg gcuucgggag ccugggacuc gauugugaac cucgcaccgg ccuggacuuu     1200

uccgaucuuu auuaccugac aaugaauaac aagcacuggc ucgugcauaa agagugguuc     1260

cacgacaucc cccugccuug gcacgccggg gcugauaccg gaacacccca uuggaauaac     1320

aaggaagccc uggucgaguu uaaagacgcg cacgcuaagc ggcagacggu ggucgugcuc     1380

ggcucucagg agggggccgu ucauaccgcc cuggcuggcg cccuugaagc ugagauggau     1440

ggagccaagg gcagacugag cuccgggcac cucaaaugcc gccugaagau ggacaaacuu     1500

cggcugaagg gagugagcua uucucucugu acagcggccu ucaccuuuac aaaaauuccg     1560

gcugaaaccc ugcauggcac ggucaccgug gagguucagu acgccgggac agacggaccu     1620

ugcaaggugc ccgcucaaau ggccgucgau augcagaccc ugacaccugu gggcagacuc     1680

aucaccgcga accccgugau cacggaaucc acagagaaua gcaagaugau gcuggagcuu     1740

gaucccccuu ucggggacuc uuacauuguu aucggcgucg gagagaaaaa gauuacccac     1800

cacuggcauc gcuccggcag cacaaucggg aaagccuuug aagcuaccgu gcggggagcc     1860

aagagaaugg cugugcuggg cgacaccgcc ugggauuucg gguccgucgg aggcgcccuc     1920

aacucucugg ggaaaggcau ucaccagauc uucggagcug cguuuaagag ccuguucggc     1980

gggauguccu gguuuucuca aauccuuauu ggaacacucc ugauguggcu gggccucaau     2040

acgaagaacg ggagcaucuc ccugaugugu cuugcccugg gaggcgugcu cauuuuccug     2100

agcaccgcug uuucugccga cguggggugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  4443
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4443
gggagaaagc uuaccaugaa cgccagaaag gagaaaaagc ggcgcggcgc ugacaccagc       60

guggggaucg ucggacugcu ccugacaacg gccauggcgg cugaaguuac cagacggggc      120

uccgccuacu auauguaccu ugaucgcaau gacgccgggg aggcuauuuc uuuccccaca      180

acccugggaa ugaacaaaug cuauauccag auuauggauc ucggccacau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggggugg aaccggacga uguggacugu      300

uggugcaaua ccacauccac cugggucgug uauggaacgu gucaucacaa gaaaggcgag      360

gcuagacggu cucgcagagc cguuacccuu cccagccauu ccacacggaa gcugcaaacc      420

cgcucucaga cauggcucga gagcagagaa uacaccaagc accugauccg gguggagaac      480

uggauuuuuc gcaauccugg cuucgcgcug gccgcugccg cuaucgccug gcuccugggg      540

uccagcacgu cucagaaagu caucuaucuu gugaugauuc ugcucaucgc gcccgccuac      600

uccauuagau gcaucggagu uagcaaccgg gauuuugugg aaggcauguc ugggggaaca      660

ugggucgaca uugugcugga gcauggcggg ugugugaccg ucauggcuca ggauaagccu      720

acaguugaca ucgagcuugu gaccaccaca guguccaaua uggccgaagu ccgcagcuac      780

ugcuaugagg cuucuauuuc cgauauggcc agcgacucca gaugucccac gcaaggagaa      840

gccuaccugg acaaacaguc ugauacccag uaugugugca agcggacacu cguugacaga      900

ggcuggggca acgggugugg acuguucggc aaagggagcc uggugaccug cgcuaaguuu      960

gcgugcucca agaaaaugac aggaaagagc auccagccug agaaucucga auaccgcauc     1020

augcugucug uccacggcuc ccaacacagc gggaugauug ugaacgauac cggacaugag     1080

acggacgaga aucgggccaa aguugaaauc acaccgaacu cucccagagc ugaggccacc     1140

cuuggcggcu ucgggagccu gggacucgau ugugaaccuc gcaccggccu ggacuuuucc     1200

gaucuuuauu accugacaau gaauaacaag cacuggcucg ugcauaaaga gugguuccac     1260

gacauucccc ugccuuggca cgccggggcu gauaccggaa caccccauug gaauaacaag     1320

gaagcccugg ucgaguuuaa agacgcgcac gcuaagcggc agacgguggu cgugcucggc     1380

ucucaggagg gggccguuca uaccgcccug gcuggcgccc uugaagcuga gauggaugga     1440

gccaagggca gacugagcuc cgggcaccuc aaaugccgcc ugaagaugga caaacuucgg     1500

cugaagggag ugagcuauuc ucucuguaca gcggccuuca ccuuuacaaa aaucccggcu     1560

gaaacccugc auggcacggu caccguggag guucaguacg ccgggacaga cggaccuugc     1620

aaggugcccg cucaaauggc cgucgauaug cagacccuga caccuguggg cagacucauu     1680

accgcgaacc ccgugaucac ggaauccaca gagaauagca agaugaugcu ggagcuugau     1740

cccccuuucg gggacucuua caucguuauu ggcgucggag agaaaaagau cacccaccac     1800

uggcaucgcu ccggcagcac aauugggaaa gccuuugaag cuaccgugcg gggagccaag     1860

agaauggcug ugcugggcga caccgccugg gauuucgggu ccgucggagg cgcccucaac     1920

ucucugggga aaggcaucca ccagauuuuc ggagcugcgu uuaagagccu guucggcggg     1980

auguccuggu uuucucaaau ccuuaucgga acacuccuga uguggcuggg ccucaauacg     2040

aagaacggga gcauuucccu gaugugucuu gcccugggag gcgugcucau cuuccugagc     2100

accgcuguuu cugccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4444
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4444
gggagaaagc uuaccauggc cagaaaggag aaaaagcggc gcggcgcuga caccagcgug       60

gggaucgucg gacugcuccu gacaacggcc auggcggcug aaguuaccag acggggcucc      120

gccuacuaua uguaccuuga ucgcaacgac gccggggagg cuauuucuuu ccccacaacc      180

cugggaauga auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca      240

augagcuacg aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg      300

ugcaacacca cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu      360

agacggucuc gcagagccgu uacccuuccc agccauucca cacggaagcu gcaaacccgc      420

ucucagacau ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaauugg      480

auuuuucgca acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc      540

agcacgucuc agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc      600

auuagaugca ucggaguuag caaucgggau uuuguggaag gcaugucugg gggaacaugg      660

gucgacauug ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca      720

guugacaucg agcuugugac caccacagug uccaacaugg ccgaaguccg cagcuacugc      780

uaugaggcuu cuauuuccga uauggccagc gacuccagau gucccacgca aggagaagcc      840

uaccuggaca aacagucuga uacccaguau gugugcaagc ggacacucgu ugacagaggc      900

uggggcaaug gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg      960

ugcuccaaga aaaugacagg aaagagcauc cagccugaga accucgaaua ccgcaucaug     1020

cugucugucc acggcuccca acacagcggg augauuguga augauaccgg acaugagacg     1080

gacgagaacc gggccaaagu ugaaaucaca cccaauucuc ccagagcuga ggccacccuu     1140

ggcggcuucg ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau     1200

cuuuauuacc ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac     1260

auuccccugc cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa     1320

gcccuggucg aguuuaaaga cgcgcacgcu aagcggcaga cgguggucgu gcucggcucu     1380

caggaggggg ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc     1440

aagggcagac ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa     1560

acccugcaug gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag     1620

gugcccgcuc aaauggccgu cgauaugcag acccugacac cugugggcag acucauuacc     1680

gcgaaccccg ugaucacgga auccacagag aacagcaaga ugaugcugga gcuugacccg     1740

ccuuucgggg auucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaagcc uuugaagcua ccgugcgggg agccaagaga     1860

auggcugugc ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaauucu     1920

cuggggaaag gcauccacca gauuuucgga gcugcguuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag     2040

aaugggagca uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc     2100

gcuguuucug ccgacguggg gugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4445
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4445
gggagaaagc uuaccaugag aaaggagaaa aagcggcgcg gcgccgacac cagcgugggg       60

aucgucggac ugcuccugac aacggcuaug gccgcggaag uuaccagacg gggcuccgcu      120

uacuauaugu accuugaucg caacgacgcc ggggaggcca uuucuuuccc cacaacccug      180

ggaaugaaua aaugcuauau ccagauuaug gaucucggcc acauguguga cgcuacaaug      240

agcuacgaau gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc      300

aacaccacau ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggccaga      360

cggucucgca gagcuguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu      420

cagacauggc ucgagagcag agaauacacc aagcaccuga uccgggugga gaauuggauu      480

uuucgcaacc cuggcuucgc ccuggcggcc gcugccaucg cuuggcuccu gggguccagc      540

acgucucaga aagucaucua ucuugugaug auucugcuca ucgcccccgc guacuccauu      600

agaugcaucg gaguuagcaa ucgggauuuu guggaaggca ugucuggggg aacauggguc      660

gacauugugc uggagcaugg cgggugugug accgucaugg cccaggauaa gccuacaguu      720

gacaucgagc uugugaccac cacagugucc aacauggcug aaguccgcag cuacugcuau      780

gaggccucua uuuccgauau ggcuagcgac uccagauguc ccacgcaagg agaagccuac      840

cuggacaaac agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg      900

ggcaaugggu guggacuguu cggcaaaggg agccugguga ccugcgccaa guuugcuugc      960

uccaagaaaa ugacaggaaa gagcauccag ccugagaacc ucgaauaccg caucaugcug     1020

ucuguccacg gcucccaaca cagcgggaug auugugaaug auaccggaca ugagacggac     1080

gagaaccggg cgaaaguuga aaucacaccc aauucuccca gagccgaggc uacccuuggc     1140

ggcuucggga gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu     1200

uauuaccuga caaugaacaa uaagcacugg cucgugcaua aagagugguu ccacgacauu     1260

ccccugccuu ggcacgccgg ggccgauacc ggaacacccc auuggaacaa uaaggaagcu     1320

cuggucgagu uuaaagacgc ccacgcgaag cggcagacgg uggucgugcu cggcucucag     1380

gagggggcug uucauaccgc ccuggccggc gcucuugaag ccgagaugga uggagcuaag     1440

ggcagacuga gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag     1500

ggagugagcu auucucucug uacagccgcg uucaccuuua caaaaauccc ggccgaaacc     1560

cugcauggca cggucaccgu ggagguucag uacgcuggga cagacggacc uugcaaggug     1620

cccgcccaaa uggcugucga uaugcagacc cugacaccug ugggcagacu cauuaccgcc     1680

aaccccguga ucacggaauc cacagagaac agcaagauga ugcuggagcu ugacccgccu     1740

uucggggauu cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau     1800

cgcuccggca gcacaauugg gaaagcguuu gaagccaccg ugcggggagc uaagagaaug     1860

gccgugcugg gcgacaccgc uugggauuuc ggguccgucg gaggcgcccu caauucucug     1920

gggaaaggca uccaccagau uuucggagcc gcuuuuaaga gccuguucgg cgggaugucc     1980

ugguuuucuc aaauccuuau cggaacacuc cugauguggc ugggccucaa cacgaagaau     2040

gggagcauuu cccugaugug ucuugcgcug ggaggcgugc ucaucuuccu gagcaccgcc     2100

guuucugcug acguggggug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4446
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4446
gggagaaagc uuaccaugaa ggagaaaaag agacggggcg ccgacaccag cguggggauc       60

gucggacugc uccugacaac ggcuauggcc gcggaaguua cccgcagagg cuccgcuuac      120

uauauguacc uugaucggaa cgacgccggg gaggccauuu cuuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcccgcaga      360

ucucggcgcg cuguuacccu ucccagccau uccacaagaa agcugcaaac ccggucucag      420

acauggcucg agagccgcga auacaccaag caccugauca gaguggagaa uuggauuuuu      480

cggaacccug gcuucgcccu ggcggccgcu gccaucgcuu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cuccauucgc      600

ugcaucggag uuagcaauag agauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggccc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaac auggcugaag uccggagcua cugcuaugag      780

gccucuauuu ccgauauggc uagcgacucc cgcuguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg gggcuggggc      900

aaugggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaccucg aauacagaau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaaugaua ccggacauga gacggacgag     1080

aaccgcgcga aaguugaaau cacacccaau ucuccccggg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu agaaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagcgc cagacggugg ucgugcucgg cucucaggag     1380

ggggcuguuc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cggcugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccggcucau uaccgccaac     1680

cccgugauca cggaauccac agagaacagc aagaugaugc uggagcuuga cccgccuuuc     1740

ggggauucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcauaga     1800

uccggcagca caauugggaa agcguuugaa gccaccgugc gcggagcuaa gcggauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccgucggag gcgcccucaa uucucugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugcugacg uggggugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4447
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4447
gggagaaagc uuaccaugga gaagaaaaga cggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc uauggccgcg gaaguuaccc gcagaggcuc cgcuuacuau      120

auguaccuug aucggaacga cgccggggag gccauuucuu uccccacaac ccugggaaug      180

aauaagugcu auauccagau uauggaucuc ggccacaugu gugacgcuac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaacacc      300

acauccaccu gggucgugua uggaacgugu caucacaaaa agggcgaggc ccgcagaucu      360

cggcgcgcug uuacccuucc cagccauucc acaagaaaac ugcaaacccg gucucagaca      420

uggcucgaga gccgcgaaua caccaagcac cugaucagag uggagaauug gauuuuucgg      480

aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc uccugggguc cagcacgucu      540

cagaagguca ucuaucuugu gaugauucug cucaucgccc ccgcguacuc cauucgcugc      600

aucggaguua gcaauagaga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcccagg auaaaccuac aguugacauc      720

gagcuuguga ccaccacagu guccaacaug gcugaagucc ggagcuacug cuaugaggcc      780

ucuauuuccg auauggcuag cgacucccgc ugucccacgc aaggagaagc cuaccuggac      840

aagcagucug auacccagua ugugugcaaa agaacacucg uugaccgggg cuggggcaau      900

ggguguggac uguucggcaa ggggagccug gugaccugcg ccaaauuugc uugcuccaag      960

aagaugacag gaaaaagcau ccagccugag aaccucgaau acagaaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aaugauaccg gacaugagac ggacgagaac     1080

cgcgcgaagg uugaaaucac acccaauucu ccccgggccg aggcuacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccuaga accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga acaauaaaca cuggcucgug cauaaggagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggccga uaccggaaca ccccauugga acaauaaaga agcucugguc     1320

gaguuuaagg acgcccacgc gaaacgccag acgguggucg ugcucggcuc ucaggagggg     1380

gcuguucaua ccgcccuggc cggcgcucuu gaagccgaga uggauggagc uaagggccgg     1440

cugagcuccg ggcaccucaa gugcagacug aaaauggaca aacuucgccu gaagggagug     1500

agcuauucuc ucuguacagc cgcguucacc uuuacaaaga ucccggccga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcu gggacagacg gaccuugcaa agugcccgcc     1620

caaauggcug ucgauaugca gacccugaca ccugugggcc ggcucauuac cgccaacccc     1680

gugaucacgg aauccacaga gaacagcaag augaugcugg agcuugaccc gccuuucggg     1740

gauucuuaca ucguuauugg cgucggagaa aagaaaauca cccaccacug gcauagaucc     1800

ggcagcacaa uugggaaggc guuugaggcc accgugcgcg gagcuaaacg gauggccgug     1860

cugggcgaca ccgcuuggga uuucgggucc gucggaggcg cccucaauuc ucuggggaag     1920

ggcauccacc agauuuucgg agccgcuuuu aaaagccugu ucggcgggau guccugguuu     1980

ucucaaaucc uuaucggaac acuccugaug uggcugggcc ucaacacgaa gaaugggagc     2040

auuucccuga ugugucuugc gcugggaggc gugcucaucu uccugagcac cgccguuucu     2100

gcugacgugg ggugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4448
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4448
gggagaaagc uuaccaugaa gaaaagacgg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuga caacggcuau ggccgcggag guuacccgca gaggcuccgc uuacuauaug      120

uaccuugauc ggaacgacgc cggggaagcc auuucuuucc ccacaacccu gggaaugaau      180

aagugcuaua uccagauuau ggaucucggc cacaugugug acgcuacaau gagcuacgag      240

ugcccuaugc uggaugaagg gguggagccg gacgaugugg acuguuggug caacaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaaaaagg gcgaagcccg cagaucucgg      360

cgcgcuguua cccuucccag ccauuccaca agaaaacugc aaacccgguc ucagacaugg      420

cucgagagcc gcgaguacac caagcaccug aucagagugg aaaauuggau uuuucggaac      480

ccuggcuucg cccuggcggc cgcugccauc gcuuggcucc ugggguccag cacgucucag      540

aaggucaucu aucuugugau gauucugcuc aucgcccccg cguacuccau ucgcugcauc      600

ggaguuagca auagagauuu uguggagggc augucugggg gaacaugggu cgacauugug      660

cuggaacaug gcgggugugu gaccgucaug gcccaggaua aaccuacagu ugacaucgag      720

cuugugacca ccacaguguc caacauggcu gagguccgga gcuacugcua ugaagccucu      780

auuuccgaua uggcuagcga cucccgcugu cccacgcaag gagaggccua ccuggacaag      840

cagucugaua cccaguaugu gugcaaaaga acacucguug accggggcug gggcaauggg      900

uguggacugu ucggcaaggg gagccuggug accugcgcca aauuugcuug cuccaagaag      960

augacaggaa aaagcaucca gccugaaaac cucgaguaca gaaucaugcu gucuguccac     1020

ggcucccaac acagcgggau gauugugaau gauaccggac augaaacgga cgagaaccgc     1080

gcgaagguug agaucacacc caauucuccc cgggccgaag cuacccuugg cggcuucggg     1140

agccugggac ucgauuguga gccuagaacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaca auaaacacug gcucgugcau aaggaauggu uccacgacau uccccugccu     1260

uggcacgccg gggccgauac cggaacaccc cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggacg cccacgcgaa acgccagacg guggucgugc ucggcucuca ggagggggcu     1380

guucauaccg cccuggccgg cgcucuugag gccgaaaugg auggagcuaa gggccggcug     1440

agcuccgggc accucaagug cagacugaaa auggacaaac uucgccugaa gggagugagc     1500

uauucucucu guacagccgc guucaccuuu acaaagaucc cggccgagac ccugcauggc     1560

acggucaccg uggaaguuca guacgcuggg acagacggac cuugcaaagu gcccgcccaa     1620

auggcugucg auaugcagac ccugacaccu gugggccggc ucauuaccgc caaccccgug     1680

aucacggagu ccacagaaaa cagcaagaug augcuggagc uugacccgcc uuucggggau     1740

ucuuacaucg uuauuggcgu cggagagaag aaaaucaccc accacuggca uagauccggc     1800

agcacaauug ggaaggcguu ugaagccacc gugcgcggag cuaaacggau ggccgugcug     1860

ggcgacaccg cuugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaagggc     1920

auccaccaga uuuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu     2040

ucccugaugu gucuugcgcu gggaggcgug cucaucuucc ugagcaccgc cguuucugcu     2100

gacguggggu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4449
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4449
gggagaaagc uuaccaugaa gagacggggc gccgacacca gcguggggau cgucggacug       60

cuccugacaa cggcuauggc cgcggagguu acccgcagag gcuccgcuua cuauauguac      120

cuugaucgga acgacgccgg ggaagccauu ucuuucccca caacccuggg aaugaauaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg cuacaaugag cuacgagugc      240

ccuaugcugg augaaggggu ggagccggac gauguggacu guuggugcaa caccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aagcccgcag aucucggcgc      360

gcuguuaccc uucccagcca uuccacaaga aagcugcaaa cccggucuca gacauggcuc      420

gagagccgcg aguacaccaa acaccugauc agaguggaaa auuggauuuu ucggaacccu      480

ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaag      540

gucaucuauc uugugaugau ucugcucauc gcccccgcgu acuccauucg cugcaucgga      600

guuagcaaua gagauuuugu ggagggcaug ucugggggaa caugggucga cauugugcug      660

gaacauggcg ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa cauggcugag guccggagcu acugcuauga agccucuauu      780

uccgauaugg cuagcgacuc ccgcuguccc acgcaaggag aggccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagagaaca cucguugacc ggggcugggg caaugggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caaaaagaug      960

acaggaaaga gcauccagcc ugaaaaccuc gaguacagaa ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaaugau accggacaug aaacggacga gaaccgcgcg     1080

aaaguugaga ucacacccaa uucuccccgg gccgaagcua cccuuggcgg cuucgggagc     1140

cugggacucg auugugagcc uagaaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaacaaua agcacuggcu cgugcauaaa gaaugguucc acgacauucc ccugccuugg     1260

cacgccgggg ccgauaccgg aacaccccau uggaacaaua aggaggcucu ggucgaauuu     1320

aaagacgccc acgcgaagcg ccagacggug gucgugcucg gcucucagga gggggcuguu     1380

cauaccgccc uggccggcgc ucuugaggcc gaaauggaug gagcuaaagg ccggcugagc     1440

uccgggcacc ucaagugcag acugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucucucugua cagccgcguu caccuuuaca aaaaucccgg ccgagacccu gcauggcacg     1560

gucaccgugg aaguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug     1620

gcugucgaua ugcagacccu gacaccugug ggccggcuca uuaccgccaa ccccgugauc     1680

acggagucca cagaaaacag caaaaugaug cuggagcuug acccgccuuu cggggauucu     1740

uacaucguua uuggcgucgg agagaagaag aucacccacc acuggcauag auccggcagc     1800

acaauuggga aagcguuuga agccaccgug cgcggagcua agcggauggc cgugcugggc     1860

gacaccgcuu gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaca cgaaaaaugg gagcauuucc     2040

cugauguguc uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac     2100

guggggugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4450
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4450
gggagaaagc uuaccaugag acggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg cuauggccgc ggagguuacc cgcagaggcu ccgcuuacua uauguaccuu      120

gaucggaacg acgccgggga agccauuucu uuccccacaa cccugggaau gaauaagugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcua caaugagcua cgagugcccu      240

augcuggaug aaggggugga gccggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaaa aagggcgaag cccgcagauc ucggcgcgcu      360

guuacccuuc ccagccauuc cacaagaaaa cugcaaaccc ggucucagac auggcucgag      420

agccgcgagu acaccaagca ccugaucaga guggaaaauu ggauuuuucg gaacccuggc      480

uucgcccugg cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu      600

agcaauagag auuuugugga gggcaugucu gggggaacau gggucgacau ugugcuggaa      660

cauggcgggu gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggcugagguc cggagcuacu gcuaugaagc cucuauuucc      780

gauauggcua gcgacucccg cugucccacg caaggagagg ccuaccugga caagcagucu      840

gauacccagu augugugcaa aagaacacuc guugaccggg gcuggggcaa ugggugugga      900

cuguucggca aggggagccu ggugaccugc gccaaauuug cuugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga aaaccucgag uacagaauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaaa cggacgagaa ccgcgcgaag     1080

guugagauca cacccaauuc uccccgggcc gaagcuaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugagccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccccu gccuuggcac     1260

gccggggccg auaccggaac accccauugg aacaauaaag aggcucuggu cgaauuuaag     1320

gacgcccacg cgaaacgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau     1380

accgcccugg ccggcgcucu ugaggccgaa auggauggag cuaagggccg gcugagcucc     1440

gggcaccuca aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu     1500

cucuguacag ccgcguucac cuuuacaaag aucccggccg agacccugca uggcacgguc     1560

accguggaag uucaguacgc ugggacagac ggaccuugca aagugcccgc ccaaauggcu     1620

gucgauaugc agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca     1800

auugggaagg cguuugaagc caccgugcgc ggagcuaaac ggauggccgu gcugggcgac     1860

accgcuuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa gggcauccac     1920

cagauuuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacgug     2100

gggugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4451
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4451
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga gguuacccgg cgcggcuccg cuuacuauau guaccuugau      120

agaaacgacg ccggggaagc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga gugcccuaug      240

cuggaugaag ggguggagcc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaagccc ggcgcucuag acgggcuguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgagagc      420

cgggaguaca ccaagcaccu gauccgcgug gaaaauugga uuuuuagaaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaggg caugucuggg ggaacauggg ucgacauugu gcuggaacau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaggucaga agcuacugcu augaagccuc uauuuccgau      780

auggcuagcg acucccggug ucccacgcaa ggagaggccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugaaaa ccucgaguac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugaaacgg acgagaacag agcgaagguu     1080

gagaucacac ccaauucucc ccgcgccgaa gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug agccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggaaugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaagagg cucuggucga auuuaaggac     1320

gcccacgcga aaagacagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga ggccgaaaug gauggagcua agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaagauc ccggccgaga cccugcaugg cacggucacc     1560

guggaaguuc aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg ccaaccccgu gaucacggag     1680

uccacagaaa acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggcgu uugaagccac cgugagagga gcuaaacgca uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacgugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4452
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4452
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaggu uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

aacgacgccg gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug      240

gaugaagggg uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caaaaagggc gaagccagac ggucucgcag agcuguuacc      360

cuucccagcc auuccacacg gaaacugcaa acccgcucuc agacauggcu cgagagcaga      420

gaguacacca agcaccugau ccggguggaa aauuggauuu uucgcaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauua gaugcaucgg aguuagcaau      600

cgggauuuug uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga gguccgcagc uacugcuaug aagccucuau uuccgauaug      780

gcuagcgacu ccagaugucc cacgcaagga gaggccuacc uggacaagca gucugauacc      840

caguaugugu gcaaacggac acucguugac cgcggcuggg gcaaugggug uggacuguuc      900

ggcaagggga gccuggugac cugcgccaaa uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gaaacggacg agaaccgggc gaagguugag     1080

aucacaccca auucucccag agccgaagcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugagc cucgcaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc     1320

cacgcgaaac ggcagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaggc cgaaauggau ggagcuaagg gcagacugag cuccgggcac     1440

cucaaaugcc gccugaagau ggacaaacuu cggcugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug     1560

gaaguucagu acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggcagacuc auuaccgcca accccgugau cacggagucc     1680

acagaaaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcauc gcuccggcag cacaauuggg     1800

aaggcguuug aagccaccgu gcggggagcu aaaagaaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu     1920

uucggagccg cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga cguggggugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4453
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4453
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccugacaacg       60

gcuauggccg cggagguuac cagacgggga uccgcuuacu auauguaccu ugaucgcaac      120

gacgccggcg aagccauuuc uuuccccaca acccugggga ugaauaagug cuauauccag      180

auuauggauc ucggacacau gugugacgcu acaaugagcu acgagugccc uaugcuggau      240

gaaggcgugg agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uaugggacgu gucaucacaa aaagggagaa gccagacggu cucgcagagc uguuacccuu      360

cccagccauu ccacacggaa acugcaaacc cgcucucaga cauggcucga gagcagagag      420

uacaccaagc accugauccg gguggaaaau uggauuuuuc gcaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggc uccagcacgu cucagaaagu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauuagau gcaucggggu uagcaaucgg      600

gauuuugugg agggaauguc uggcgggaca ugggucgaca uugugcugga acauggaggc      660

ugugugaccg ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaggu ccgcagcuac ugcuaugaag ccucuauuuc cgauauggcu      780

agcgacucca gaugucccac gcaaggggag gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aacggacacu cguugaccgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucca agaaaaugac aggaaagagc      960

auccagccug aaaaccucga guacagaauc augcugucug uccacggguc ccaacacagc     1020

ggcaugauug ugaaugauac cgggcaugaa acggacgaga accgggcgaa gguugagauc     1080

acacccaauu cucccagagc cgaagcuacc cuuggaggcu ucggcagccu ggggcucgau     1140

ugugagccuc gcaccggacu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga augguuccac gacauucccc ugccuuggca cgccggcgcc     1260

gauaccggga caccccauug gaacaauaaa gaggcucugg ucgaauuuaa ggacgcccac     1320

gcgaaacggc agacgguggu cgugcucgga ucucaggagg gcgcuguuca uaccgcccug     1380

gccggggcuc uugaggccga aauggauggc gcuaagggaa gacugagcuc cggccaccuc     1440

aaaugccgcc ugaagaugga caagcugcgg cugaaagggg ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gagacccugc auggaacggu caccguggaa     1560

guucaguacg cuggcacaga cgggccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg aagacucauu accgccaacc ccgugaucac ggaguccaca     1680

gaaaacagca agaugaugcu ggagcuugac ccgccuuucg gcgauucuua caucguuauu     1740

ggggucggcg agaaaaagau cacccaccac uggcaucgcu ccggaagcac aauuggcaag     1800

gcguuugaag ccaccgugag aggggcuaaa cggauggccg ugcugggaga caccgcuugg     1860

gauuucggcu ccgucggggg agcccucaau ucucugggca aggggaucca ccagauuuuc     1920

ggcgccgcuu uuaaaagccu guucggaggc auguccuggu uuucucaaau ccuuaucggg     1980

acacuccuga uguggcuggg acucaacacg aagaauggca gcauuucccu gaugugucuu     2040

gcgcuugggg gagugcucau cuuccugagc accgccguuu cugcugacgu gggcugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4454
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4454
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu gacaacggcc       60

auggcugccg agguuaccag acggggaucc gcguacuaua uguaccuuga ucgcaacgac      120

gcuggcgaag ccauuucuuu ccccacaacc cuggggauga auaagugcua uauccagauu      180

auggaucucg gacacaugug ugacgccaca augagcuacg agugcccuau gcuggaugaa      240

ggcguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

gggacguguc aucacaaaaa gggagaagcu agacggucuc gcagagccgu uacccuuccc      360

agccauucca cacggaaacu gcaaacccgc ucucagacau ggcucgagag cagagaguac      420

accaagcacc ugauccgggu ggaaaauugg auuuuucgca acccuggcuu cgcucuggcc      480

gcggccgcua ucgccuggcu ccugggcucc agcacgucuc agaaagucau cuaucuugug      540

augauucugc ucaucgcucc cgccuacucc auuagaugca ucgggguuag caaucgggau      600

uuuguggagg gaaugucugg cgggacaugg gucgacauug ugcuggaaca uggaggcugu      660

gugaccguca uggcgcagga uaagccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg ccgagguccg cagcuacugc uaugaagcua gcauuuccga uauggccagc      780

gacuccagau gucccacgca aggggaggcu uaccuggaca agcagucuga uacccaguau      840

gugugcaaac ggacacucgu ugaccgcgga uggggcaaug gcugugggcu guucggaaag      900

ggcagccugg ugaccugcgc caaauuugcc ugcuccaaga aaaugacagg gaagucuauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggauccca acacagcggc     1020

augauuguga augauaccgg gcaugaaacg gacgagaacc gggcuaaggu ugagaucaca     1080

cccaauucuc ccagagcgga agccacccuu ggaggcuucg gcagccuggg gcucgauugu     1140

gagccucgca ccggacugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc uggcgccgau     1260

accgggacac cccauuggaa caauaaagag gcccuggucg aauuuaagga cgcucacgcc     1320

aaacggcaga cgguggucgu gcucggaucu caggagggcg cgguucauac cgcucuggcc     1380

ggggcccuug aggcugaaau ggauggcgcc aagggaagac ugagcuccgg ccaccucaaa     1440

ugccgccuga agauggacaa gcugcggcug aaagggguga gcuauucucu cuguacagcu     1500

gccuucaccu uuacaaagau cccggcggag acccugcaug gaacggucac cguggaaguu     1560

caguacgccg gcacagacgg gccuugcaaa gugcccgcuc aaauggccgu cgauaugcag     1620

acccugacac cugugggaag acucauuacc gcuaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucggcg auucuuacau cguuauuggg     1740

gucggcgaga aaaagaucac ccaccacugg caucgcuccg gaagcacaau uggcaaggcc     1800

uuugaagcga ccgugagagg ggccaaacgg auggcugugc ugggagacac cgccugggau     1860

uucggcuccg ucgggggagc ucucaauucu cugggcaagg ggauccacca gauuuucggc     1920

gccgccuuua aaagccuguu cggaggcaug uccugguuuu cucaaauccu uaucgggaca     1980

cuccugaugu ggcugggacu caacacgaag aauggcagca uuucccugau gugucuugcu     2040

cuugggggag ugcucaucuu ccugagcacc gcgguuucug ccgacguggg cugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4455
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4455
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccg augugggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4456
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4456
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgcgg uuaccagacg gggauccgcu uacuauaugu accuugaccg caacgaugcc      120

ggcgaggcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugcuacaaug agcuacgaau gcccuaugcu ggacgagggc      240

guggaaccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaggccaga cggucucgca gagcuguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgaaagcag agaguacacc      420

aagcaccuga uccgggugga gaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggaaggaa ugucuggcgg gacauggguc gauauugugc uggagcaugg aggcugugug      660

accgucaugg cccaggacaa gccuacaguu gauaucgaac uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaggccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaagccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauccag      960

ccugagaacc ucgaauacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugagacggau gaaaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaggc uacccuugga ggcuucggca gccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccccugccuu ggcacgccgg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaagcu cuggucgagu uuaaggaugc ccacgcgaaa     1320

cggcagacgg uggucgugcu cggaucucag gaaggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgagaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgaaacc cugcauggaa cggucaccgu ggagguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaggccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcug augugggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4457
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4457
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcggc uguuacccgg cgcggcuccg ccuacuauau guaccuugau      120

agaaacgacg ccggggaggc uauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgccacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaggcuc ggcgcucuag acgggccguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgaaagc      420

cgggaguaca ccaagcaccu gauccgcgug gagaauugga uuuuuagaaa cccuggcuuc      480

gcgcuggccg cugccgcuau cgccuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgcgccc gccuacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcucaggau aagccuacag uugacaucga acuugugacc      720

accacagugu ccaacauggc cgaggucaga agcuacugcu augaggcuuc uauuuccgau      780

auggccagcg acucccggug ucccacgcaa ggagaagccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcu aaauuugcgu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgaaaacag agccaagguu     1080

gagaucacac ccaauucucc ccgcgcugag gccacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggcugaua ccggaacacc ccauuggaac aauaaagaag cccuggucga guuuaaggac     1320

gcgcacgcua aaagacagac gguggucgug cucggcucuc aggaaggggc cguucauacc     1380

gcccuggcug gcgcccuuga ggcugagaug gauggagcca agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagcgg ccuucaccuu uacaaagauc ccggcugaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgccgg gacagacgga ccuugcaaag ugcccgcuca aauggccguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg cgaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggaa cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggccu uugaggcuac cgugagagga gccaaacgca uggcugugcu gggcgacacc     1860

gccugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag cugcguuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugccc ugggaggcgu gcucaucuuc cugagcaccg cuguuucugc cgacgugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4458
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4458
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gaggcguuac gcggcgcggg      120

agcgccuacu auauguaccu ggauagaaac gacgcuggag aggccauuuc cuuucccacc      180

acacuuggca ugaauaaaug cuauauccaa auuauggauc uggggcauau gugugacgcg      240

accaugucuu acgaaugccc uaugcucgau gagggagugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggcaccu gucaccauaa gaaaggcgag      360

gcucggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg guuugcccuu gcugccgcug ccaucgcgug gcugcucgga      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ccccgcuuac      600

ucuauucggu gcaucggcgu uuccaaucgc gauuucgugg aagggaugag cggaggcacg      660

ugggucgaca uugugcucga gcacggggga ugugugacag ucauggccca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggcugaggu cagauccuac      780

ugcuaugagg ccagcauuuc cgauauggcg ucugacagcc ggugucccac acagggcgaa      840

gccuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggga auggaugugg ccuguuuggg aaaggaagcc uugugacaug cgcuaaguuc      960

gccugcucua aaaagaugac cggcaagucc auccaaccug agaaccugga auaccggauc     1020

augcucagcg uccacggguc ucagcauagc ggaaugauug ugaaugauac aggccacgag     1080

accgacgaaa acagagcuaa aguugagauc acgcccaauu ccccgcgcgc cgaggccaca     1140

cugggcgggu uuggaucucu uggccuggau ugugaaccuc ggaccgggcu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcuggagcg gauacaggca caccccacug gaacaauaag     1320

gaagcccuug ucgaguucaa agacgcucau gccaagagac agacaguggu cgugcugggg     1380

ucccaggaag gcgccguuca cacggcucuc gccggagcgc uggaggcuga gauggauggc     1440

gccaaagggc gccuuagcuc uggacaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggcg uguccuauag ccucuguacc gccgcuuuua cauucaccaa aaucccggcc     1560

gaaacacugc acgggaccgu cacgguggag guucaauacg cuggaaccga cggcccuugc     1620

aaggugcccg cccagauggc ggucgauaug cagacacuua ccccuguggg gcgccugauu     1680

acagccaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gcgacagcua caucguuauu ggagucggcg agaagaagau cacacaccau     1800

uggcaccggu ccgggucuac cauuggaaag gcuuucgagg ccacagugag aggcgcuaaa     1860

cgcauggccg ugcuggggga caccgcgugg gauuucggaa gcgucggcgg ggcccuuaau     1920

ucccucggca aaggaaucca ucaaauuuuu ggcgcugccu ucaagucucu guuuggggga     1980

augagcuggu ucucccagau ccugaucggc acccuccuga uguggcuugg gcugaacacc     2040

aaaaauggaa gcauuucucu caugugucug gcucugggcg gggugcuuau cuuucucucc     2100

acggccguua gcgccgacgu gggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4459
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4459
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gagccguuac gcggcgcggc      120

agcgcuuacu auauguaccu ggauagaaac gacgccgggg aggcgauuuc cuuucccacc      180

acacuuggaa ugaauaaaug cuauauccaa auuauggauc ugggccauau gugugacgcu      240

accaugucuu acgaaugccc uaugcucgau gagggggugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggaaccu gucaccauaa gaaaggcgag      360

gcccggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg cuuugcucuu gccgcugccg cgaucgccug gcugcucggg      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ucccgccuac      600

ucuauucggu gcaucggagu uuccaaucgc gauuucgugg aaggcaugag cgggggaacg      660

ugggucgaca uugugcucga gcacggcggg ugugugacag ucauggcuca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggccgaggu cagauccuac      780

ugcuaugagg cgagcauuuc cgauauggcc ucugacagcc ggugucccac acagggagag      840

gcuuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggca augggugugg acuguuuggc aaagggagcc uugugacaug cgccaaguuc      960

gcuugcucua aaaagaugac cggaaagucc auccaaccug aaaaccugga auaccggauc     1020

augcucagcg uccacggcuc ucagcauagc gggaugauug ugaaugauac aggacacgag     1080

accgacgaaa acagagccaa aguugagauc acgcccaauu ccccgcgcgc cgaggcuaca     1140

cugggcggcu uugggucucu uggacuggau ugugaaccuc ggaccggccu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcgggggcc gauacaggaa caccccacug gaacaauaag     1320

gaagcucuug ucgaguucaa agacgcccau gccaagagac agacaguggu cgugcugggc     1380

ucccaggaag gggcuguuca cacggcccuc gcgggcgcuc uggaggccga gauggaugga     1440

gccaaaggcc gccuuagcuc ugggcaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggag uguccuauag ccucuguacc gcugccuuua cauucaccaa aaucccggcu     1560

gaaacacugc acggcaccgu cacgguggag guucaauacg ccgggaccga cggaccuugc     1620

aaggugcccg cgcagauggc cgucgauaug cagacacuua ccccuguggg ccgccugauu     1680

acagcuaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gggacagcua caucguuauu ggcgucggag agaagaagau cacacaccau     1800

uggcaccggu ccggcucuac cauugggaaa gccuucgagg cuacagugag aggagccaag     1860

cgcauggcgg ugcugggcga caccgccugg gauuucggga gcgucggagg cgcucuuaau     1920

ucccucggga aaggcaucca ucaaauuuuu ggagccgcuu ucaagucucu guuuggcggg     1980

augagcuggu ucucccagau ccugaucgga acccuccuga uguggcuugg ccugaacacc     2040

aaaaauggga gcauuucucu caugugucug gcccugggag gcgugcuuau cuuucucucc     2100

acggccguua gcgcugacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4460
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4460
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4461
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4461
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagcggcg cggcgcugac       60

accagcgugg ggaucgucgg acugcuccug acaacggcca uggcggcuga aguuaccaga      120

cggggcuccg ccuacuauau guaccuugau cgcaaugacg ccggggaggc uauuucuuuc      180

cccacaaccc ugggaaugaa caaaugcuau auccagauua uggaucucgg ccacaugugu      240

gacgccacaa ugagcuacga augcccuaug cuggaugagg ggguggaacc ggacgaugug      300

gacuguuggu gcaauaccac auccaccugg gucguguaug gaacguguca ucacaagaaa      360

ggcgaggcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      420

caaacccgcu cucagacaug gcucgagagc agagaauaca ccaagcaccu gauccgggug      480

gagaacugga ucuuucgcaa uccuggcuuc gcgcuggccg cugccgcuau ugccuggcuc      540

cuggggucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcgccc      600

gccuacucca uuagaugcau cggaguuagc aaccgggauu uuguggaagg caugucuggg      660

ggaacauggg ucgacauugu gcuggagcau ggcgggugug ugaccgucau ggcucaggau      720

aagccuacag uugacaucga gcuugugacc accacagugu ccaauauggc cgaaguccgc      780

agcuacugcu augaggcuuc uaucuccgau auggccagcg acuccagaug ucccacgcaa      840

ggagaagccu accuggacaa acagucugau acccaguaug ugugcaagcg gacacucguu      900

gacagaggcu ggggcaacgg guguggacug uucggcaaag ggagccuggu gaccugcgcu      960

aaguuugcgu gcuccaagaa aaugacagga aagagcauuc agccugagaa ucucgaauac     1020

cgcaucaugc ugucugucca cggcucccaa cacagcggga ugauugugaa cgauaccgga     1080

caugagacgg acgagaaucg ggccaaaguu gaaaucacac cgaacucucc cagagcugag     1140

gccacccuug gcggcuucgg gagccuggga cucgauugug aaccucgcac cggccuggac     1200

uuuuccgauc uuuauuaccu gacaaugaau aacaagcacu ggcucgugca uaaagagugg     1260

uuccacgaca uuccccugcc uuggcacgcc ggggcugaua ccggaacacc ccauuggaau     1320

aacaaggaag cccuggucga guuuaaagac gcgcacgcua agcggcagac gguggucgug     1380

cucggcucuc aggagggggc cguucauacc gcccuggcug gcgcccuuga agcugagaug     1440

gauggagcca agggcagacu gagcuccggg caccucaaau gccgccugaa gauggacaaa     1500

cuucggcuga agggagugag cuauucucuc uguacagcgg ccuucaccuu uacaaaaauc     1560

ccggcugaaa cccugcaugg cacggucacc guggagguuc aguacgccgg gacagacgga     1620

ccuugcaagg ugcccgcuca aauggccguc gauaugcaga cccugacacc ugugggcaga     1680

cucaucaccg cgaaccccgu gauuacggaa uccacagaga auagcaagau gaugcuggag     1740

cuugaucccc cuuucgggga cucuuacauc guuauuggcg ucggagagaa aaagaucacc     1800

caccacuggc aucgcuccgg cagcacaauu gggaaagccu uugaagcuac cgugcgggga     1860

gccaagagaa uggcugugcu gggcgacacc gccugggauu ucggguccgu cggaggcgcc     1920

cucaacucuc uggggaaagg cauccaccag aucuucggag cugcguuuaa gagccuguuc     1980

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacac uccugaugug gcugggccuc     2040

aauacgaaga acgggagcau uucccugaug ugucuugccc ugggaggcgu gcucaucuuc     2100

cugagcaccg cuguuucugc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4462
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4462
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agcggcgcgg cgcugacacc       60

agcgugggga uugucggacu gcuccugaca acggccaugg cggcugaagu uaccagacgg      120

ggcuccgccu acuauaugua ccuugaucgc aaugacgccg gggaggcuau cucuuucccc      180

acaacccugg gaaugaacaa augcuauauu cagaucaugg aucucggcca caugugugac      240

gccacaauga gcuacgaaug cccuaugcug gaugaggggg uggaaccgga cgauguggac      300

uguuggugca auaccacauc caccuggguc guguauggaa cgugucauca caagaaaggc      360

gaggcuagac ggucucgcag agccguuacc cuucccagcc auuccacacg gaagcugcaa      420

acccgcucuc agacauggcu cgagagcaga gaauacacca agcaccugau ucggguggag      480

aacuggaucu uucgcaaucc uggcuucgcg cuggccgcug ccgcuaucgc cuggcuccug      540

ggguccagca cgucucagaa agucauuuau cuugugauga uccugcucau ugcgcccgcc      600

uacuccauca gaugcauugg aguuagcaac cgggauuuug uggaaggcau gucuggggga      660

acaugggucg acaucgugcu ggagcauggc ggguguguga ccgucauggc ucaggauaag      720

ccuacaguug acauugagcu ugugaccacc acagugucca auauggccga aguccgcagc      780

uacugcuaug aggcuucuau cuccgauaug gccagcgacu ccagaugucc cacgcaagga      840

gaagccuacc uggacaaaca gucugauacc caguaugugu gcaagcggac acucguugac      900

agaggcuggg gcaacgggug uggacuguuc ggcaaaggga gccuggugac cugcgcuaag      960

uuugcgugcu ccaagaaaau gacaggaaag agcauccagc cugagaaucu cgaauaccgc     1020

auuaugcugu cuguccacgg cucccaacac agcgggauga ucgugaacga uaccggacau     1080

gagacggacg agaaucgggc caaaguugaa auuacaccga acucucccag agcugaggcc     1140

acccuuggcg gcuucgggag ccugggacuc gauugugaac cucgcaccgg ccuggacuuu     1200

uccgaucuuu auuaccugac aaugaauaac aagcacuggc ucgugcauaa agagugguuc     1260

cacgacaucc cccugccuug gcacgccggg gcugauaccg gaacacccca uuggaauaac     1320

aaggaagccc uggucgaguu uaaagacgcg cacgcuaagc ggcagacggu ggucgugcuc     1380

ggcucucagg agggggccgu ucauaccgcc cuggcuggcg cccuugaagc ugagauggau     1440

ggagccaagg gcagacugag cuccgggcac cucaaaugcc gccugaagau ggacaaacuu     1500

cggcugaagg gagugagcua uucucucugu acagcggccu ucaccuuuac aaaaauuccg     1560

gcugaaaccc ugcauggcac ggucaccgug gagguucagu acgccgggac agacggaccu     1620

ugcaaggugc ccgcucaaau ggccgucgau augcagaccc ugacaccugu gggcagacuc     1680

aucaccgcga accccgugau cacggaaucc acagagaaua gcaagaugau gcuggagcuu     1740

gaucccccuu ucggggacuc uuacauuguu aucggcgucg gagagaaaaa gauuacccac     1800

cacuggcauc gcuccggcag cacaaucggg aaagccuuug aagcuaccgu gcggggagcc     1860

aagagaaugg cugugcuggg cgacaccgcc ugggauuucg gguccgucgg aggcgcccuc     1920

aacucucugg ggaaaggcau ucaccagauc uucggagcug cguuuaagag ccuguucggc     1980

gggauguccu gguuuucuca aauccuuauu ggaacacucc ugauguggcu gggccucaau     2040

acgaagaacg ggagcaucuc ccugaugugu cuugcccugg gaggcgugcu cauuuuccug     2100

agcaccgcug uuucugccga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4463
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4463
gggagaaagc uuaccaugaa cgccagaaag gagaaaaagc ggcgcggcgc ugacaccagc       60

guggggaucg ucggacugcu ccugacaacg gccauggcgg cugaaguuac cagacggggc      120

uccgccuacu auauguaccu ugaucgcaau gacgccgggg aggcuauuuc uuuccccaca      180

acccugggaa ugaacaaaug cuauauccag auuauggauc ucggccacau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggggugg aaccggacga uguggacugu      300

uggugcaaua ccacauccac cugggucgug uauggaacgu gucaucacaa gaaaggcgag      360

gcuagacggu cucgcagagc cguuacccuu cccagccauu ccacacggaa gcugcaaacc      420

cgcucucaga cauggcucga gagcagagaa uacaccaagc accugauccg gguggagaac      480

uggauuuuuc gcaauccugg cuucgcgcug gccgcugccg cuaucgccug gcuccugggg      540

uccagcacgu cucagaaagu caucuaucuu gugaugauuc ugcucaucgc gcccgccuac      600

uccauuagau gcaucggagu uagcaaccgg gauuuugugg aaggcauguc ugggggaaca      660

ugggucgaca uugugcugga gcauggcggg ugugugaccg ucauggcuca ggauaagccu      720

acaguugaca ucgagcuugu gaccaccaca guguccaaua uggccgaagu ccgcagcuac      780

ugcuaugagg cuucuauuuc cgauauggcc agcgacucca gaugucccac gcaaggagaa      840

gccuaccugg acaaacaguc ugauacccag uaugugugca agcggacacu cguugacaga      900

ggcuggggca acgggugugg acuguucggc aaagggagcc uggugaccug cgcuaaguuu      960

gcgugcucca agaaaaugac aggaaagagc auccagccug agaaucucga auaccgcauc     1020

augcugucug uccacggcuc ccaacacagc gggaugauug ugaacgauac cggacaugag     1080

acggacgaga aucgggccaa aguugaaauc acaccgaacu cucccagagc ugaggccacc     1140

cuuggcggcu ucgggagccu gggacucgau ugugaaccuc gcaccggccu ggacuuuucc     1200

gaucuuuauu accugacaau gaauaacaag cacuggcucg ugcauaaaga gugguuccac     1260

gacauucccc ugccuuggca cgccggggcu gauaccggaa caccccauug gaauaacaag     1320

gaagcccugg ucgaguuuaa agacgcgcac gcuaagcggc agacgguggu cgugcucggc     1380

ucucaggagg gggccguuca uaccgcccug gcuggcgccc uugaagcuga gauggaugga     1440

gccaagggca gacugagcuc cgggcaccuc aaaugccgcc ugaagaugga caaacuucgg     1500

cugaagggag ugagcuauuc ucucuguaca gcggccuuca ccuuuacaaa aaucccggcu     1560

gaaacccugc auggcacggu caccguggag guucaguacg ccgggacaga cggaccuugc     1620

aaggugcccg cucaaauggc cgucgauaug cagacccuga caccuguggg cagacucauu     1680

accgcgaacc ccgugaucac ggaauccaca gagaauagca agaugaugcu ggagcuugau     1740

cccccuuucg gggacucuua caucguuauu ggcgucggag agaaaaagau cacccaccac     1800

uggcaucgcu ccggcagcac aauugggaaa gccuuugaag cuaccgugcg gggagccaag     1860

agaauggcug ugcugggcga caccgccugg gauuucgggu ccgucggagg cgcccucaac     1920

ucucugggga aaggcaucca ccagauuuuc ggagcugcgu uuaagagccu guucggcggg     1980

auguccuggu uuucucaaau ccuuaucgga acacuccuga uguggcuggg ccucaauacg     2040

aagaacggga gcauuucccu gaugugucuu gcccugggag gcgugcucau cuuccugagc     2100

accgcuguuu cugccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4464
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4464
gggagaaagc uuaccauggc cagaaaggag aaaaagcggc gcggcgcuga caccagcgug       60

gggaucgucg gacugcuccu gacaacggcc auggcggcug aaguuaccag acggggcucc      120

gccuacuaua uguaccuuga ucgcaacgac gccggggagg cuauuucuuu ccccacaacc      180

cugggaauga auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca      240

augagcuacg aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg      300

ugcaacacca cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu      360

agacggucuc gcagagccgu uacccuuccc agccauucca cacggaagcu gcaaacccgc      420

ucucagacau ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaauugg      480

auuuuucgca acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc      540

agcacgucuc agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc      600

auuagaugca ucggaguuag caaucgggau uuuguggaag gcaugucugg gggaacaugg      660

gucgacauug ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca      720

guugacaucg agcuugugac caccacagug uccaacaugg ccgaaguccg cagcuacugc      780

uaugaggcuu cuauuuccga uauggccagc gacuccagau gucccacgca aggagaagcc      840

uaccuggaca aacagucuga uacccaguau gugugcaagc ggacacucgu ugacagaggc      900

uggggcaaug gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg      960

ugcuccaaga aaaugacagg aaagagcauc cagccugaga accucgaaua ccgcaucaug     1020

cugucugucc acggcuccca acacagcggg augauuguga augauaccgg acaugagacg     1080

gacgagaacc gggccaaagu ugaaaucaca cccaauucuc ccagagcuga ggccacccuu     1140

ggcggcuucg ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau     1200

cuuuauuacc ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac     1260

auuccccugc cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa     1320

gcccuggucg aguuuaaaga cgcgcacgcu aagcggcaga cgguggucgu gcucggcucu     1380

caggaggggg ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc     1440

aagggcagac ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa     1560

acccugcaug gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag     1620

gugcccgcuc aaauggccgu cgauaugcag acccugacac cugugggcag acucauuacc     1680

gcgaaccccg ugaucacgga auccacagag aacagcaaga ugaugcugga gcuugacccg     1740

ccuuucgggg auucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaagcc uuugaagcua ccgugcgggg agccaagaga     1860

auggcugugc ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaauucu     1920

cuggggaaag gcauccacca gauuuucgga gcugcguuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag     2040

aaugggagca uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc     2100

gcuguuucug ccgacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4465
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4465
gggagaaagc uuaccaugag aaaggagaaa aagcggcgcg gcgccgacac cagcgugggg       60

aucgucggac ugcuccugac aacggcuaug gccgcggaag uuaccagacg gggcuccgcu      120

uacuauaugu accuugaucg caacgacgcc ggggaggcca uuucuuuccc cacaacccug      180

ggaaugaaua aaugcuauau ccagauuaug gaucucggcc acauguguga cgcuacaaug      240

agcuacgaau gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc      300

aacaccacau ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggccaga      360

cggucucgca gagcuguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu      420

cagacauggc ucgagagcag agaauacacc aagcaccuga uccgggugga gaauuggauu      480

uuucgcaacc cuggcuucgc ccuggcggcc gcugccaucg cuuggcuccu gggguccagc      540

acgucucaga aagucaucua ucuugugaug auucugcuca ucgcccccgc guacuccauu      600

agaugcaucg gaguuagcaa ucgggauuuu guggaaggca ugucuggggg aacauggguc      660

gacauugugc uggagcaugg cgggugugug accgucaugg cccaggauaa gccuacaguu      720

gacaucgagc uugugaccac cacagugucc aacauggcug aaguccgcag cuacugcuau      780

gaggccucua uuuccgauau ggcuagcgac uccagauguc ccacgcaagg agaagccuac      840

cuggacaaac agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg      900

ggcaaugggu guggacuguu cggcaaaggg agccugguga ccugcgccaa guuugcuugc      960

uccaagaaaa ugacaggaaa gagcauccag ccugagaacc ucgaauaccg caucaugcug     1020

ucuguccacg gcucccaaca cagcgggaug auugugaaug auaccggaca ugagacggac     1080

gagaaccggg cgaaaguuga aaucacaccc aauucuccca gagccgaggc uacccuuggc     1140

ggcuucggga gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu     1200

uauuaccuga caaugaacaa uaagcacugg cucgugcaua aagagugguu ccacgacauu     1260

ccccugccuu ggcacgccgg ggccgauacc ggaacacccc auuggaacaa uaaggaagcu     1320

cuggucgagu uuaaagacgc ccacgcgaag cggcagacgg uggucgugcu cggcucucag     1380

gagggggcug uucauaccgc ccuggccggc gcucuugaag ccgagaugga uggagcuaag     1440

ggcagacuga gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag     1500

ggagugagcu auucucucug uacagccgcg uucaccuuua caaaaauccc ggccgaaacc     1560

cugcauggca cggucaccgu ggagguucag uacgcuggga cagacggacc uugcaaggug     1620

cccgcccaaa uggcugucga uaugcagacc cugacaccug ugggcagacu cauuaccgcc     1680

aaccccguga ucacggaauc cacagagaac agcaagauga ugcuggagcu ugacccgccu     1740

uucggggauu cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau     1800

cgcuccggca gcacaauugg gaaagcguuu gaagccaccg ugcggggagc uaagagaaug     1860

gccgugcugg gcgacaccgc uugggauuuc ggguccgucg gaggcgcccu caauucucug     1920

gggaaaggca uccaccagau uuucggagcc gcuuuuaaga gccuguucgg cgggaugucc     1980

ugguuuucuc aaauccuuau cggaacacuc cugauguggc ugggccucaa cacgaagaau     2040

gggagcauuu cccugaugug ucuugcgcug ggaggcgugc ucaucuuccu gagcaccgcc     2100

guuucugcug acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4466
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4466
gggagaaagc uuaccaugaa ggagaaaaag agacggggcg ccgacaccag cguggggauc       60

gucggacugc uccugacaac ggcuauggcc gcggaaguua cccgcagagg cuccgcuuac      120

uauauguacc uugaucggaa cgacgccggg gaggccauuu cuuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcccgcaga      360

ucucggcgcg cuguuacccu ucccagccau uccacaagaa agcugcaaac ccggucucag      420

acauggcucg agagccgcga auacaccaag caccugauca gaguggagaa uuggauuuuu      480

cggaacccug gcuucgcccu ggcggccgcu gccaucgcuu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cuccauucgc      600

ugcaucggag uuagcaauag agauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggccc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaac auggcugaag uccggagcua cugcuaugag      780

gccucuauuu ccgauauggc uagcgacucc cgcuguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg gggcuggggc      900

aaugggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaccucg aauacagaau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaaugaua ccggacauga gacggacgag     1080

aaccgcgcga aaguugaaau cacacccaau ucuccccggg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu agaaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagcgc cagacggugg ucgugcucgg cucucaggag     1380

ggggcuguuc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cggcugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccggcucau uaccgccaac     1680

cccgugauca cggaauccac agagaacagc aagaugaugc uggagcuuga cccgccuuuc     1740

ggggauucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcauaga     1800

uccggcagca caauugggaa agcguuugaa gccaccgugc gcggagcuaa gcggauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccgucggag gcgcccucaa uucucugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugcugacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4467
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4467
gggagaaagc uuaccaugga gaagaaaaga cggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc uauggccgcg gaaguuaccc gcagaggcuc cgcuuacuau      120

auguaccuug aucggaacga cgccggggag gccauuucuu uccccacaac ccugggaaug      180

aauaagugcu auauccagau uauggaucuc ggccacaugu gugacgcuac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaacacc      300

acauccaccu gggucgugua uggaacgugu caucacaaaa agggcgaggc ccgcagaucu      360

cggcgcgcug uuacccuucc cagccauucc acaagaaaac ugcaaacccg gucucagaca      420

uggcucgaga gccgcgaaua caccaagcac cugaucagag uggagaauug gauuuuucgg      480

aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc uccugggguc cagcacgucu      540

cagaagguca ucuaucuugu gaugauucug cucaucgccc ccgcguacuc cauucgcugc      600

aucggaguua gcaauagaga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcccagg auaaaccuac aguugacauc      720

gagcuuguga ccaccacagu guccaacaug gcugaagucc ggagcuacug cuaugaggcc      780

ucuauuuccg auauggcuag cgacucccgc ugucccacgc aaggagaagc cuaccuggac      840

aagcagucug auacccagua ugugugcaaa agaacacucg uugaccgggg cuggggcaau      900

ggguguggac uguucggcaa ggggagccug gugaccugcg ccaaauuugc uugcuccaag      960

aagaugacag gaaaaagcau ccagccugag aaccucgaau acagaaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aaugauaccg gacaugagac ggacgagaac     1080

cgcgcgaagg uugaaaucac acccaauucu ccccgggccg aggcuacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccuaga accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga acaauaaaca cuggcucgug cauaaggagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggccga uaccggaaca ccccauugga acaauaaaga agcucugguc     1320

gaguuuaagg acgcccacgc gaaacgccag acgguggucg ugcucggcuc ucaggagggg     1380

gcuguucaua ccgcccuggc cggcgcucuu gaagccgaga uggauggagc uaagggccgg     1440

cugagcuccg ggcaccucaa gugcagacug aaaauggaca aacuucgccu gaagggagug     1500

agcuauucuc ucuguacagc cgcguucacc uuuacaaaga ucccggccga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcu gggacagacg gaccuugcaa agugcccgcc     1620

caaauggcug ucgauaugca gacccugaca ccugugggcc ggcucauuac cgccaacccc     1680

gugaucacgg aauccacaga gaacagcaag augaugcugg agcuugaccc gccuuucggg     1740

gauucuuaca ucguuauugg cgucggagaa aagaaaauca cccaccacug gcauagaucc     1800

ggcagcacaa uugggaaggc guuugaggcc accgugcgcg gagcuaaacg gauggccgug     1860

cugggcgaca ccgcuuggga uuucgggucc gucggaggcg cccucaauuc ucuggggaag     1920

ggcauccacc agauuuucgg agccgcuuuu aaaagccugu ucggcgggau guccugguuu     1980

ucucaaaucc uuaucggaac acuccugaug uggcugggcc ucaacacgaa gaaugggagc     2040

auuucccuga ugugucuugc gcugggaggc gugcucaucu uccugagcac cgccguuucu     2100

gcugacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4468
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4468
gggagaaagc uuaccaugaa gaaaagacgg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuga caacggcuau ggccgcggag guuacccgca gaggcuccgc uuacuauaug      120

uaccuugauc ggaacgacgc cggggaagcc auuucuuucc ccacaacccu gggaaugaau      180

aagugcuaua uccagauuau ggaucucggc cacaugugug acgcuacaau gagcuacgag      240

ugcccuaugc uggaugaagg gguggagccg gacgaugugg acuguuggug caacaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaaaaagg gcgaagcccg cagaucucgg      360

cgcgcuguua cccuucccag ccauuccaca agaaaacugc aaacccgguc ucagacaugg      420

cucgagagcc gcgaguacac caagcaccug aucagagugg aaaauuggau uuuucggaac      480

ccuggcuucg cccuggcggc cgcugccauc gcuuggcucc ugggguccag cacgucucag      540

aaggucaucu aucuugugau gauucugcuc aucgcccccg cguacuccau ucgcugcauc      600

ggaguuagca auagagauuu uguggagggc augucugggg gaacaugggu cgacauugug      660

cuggaacaug gcgggugugu gaccgucaug gcccaggaua aaccuacagu ugacaucgag      720

cuugugacca ccacaguguc caacauggcu gagguccgga gcuacugcua ugaagccucu      780

auuuccgaua uggcuagcga cucccgcugu cccacgcaag gagaggccua ccuggacaag      840

cagucugaua cccaguaugu gugcaaaaga acacucguug accggggcug gggcaauggg      900

uguggacugu ucggcaaggg gagccuggug accugcgcca aauuugcuug cuccaagaag      960

augacaggaa aaagcaucca gccugaaaac cucgaguaca gaaucaugcu gucuguccac     1020

ggcucccaac acagcgggau gauugugaau gauaccggac augaaacgga cgagaaccgc     1080

gcgaagguug agaucacacc caauucuccc cgggccgaag cuacccuugg cggcuucggg     1140

agccugggac ucgauuguga gccuagaacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaca auaaacacug gcucgugcau aaggaauggu uccacgacau uccccugccu     1260

uggcacgccg gggccgauac cggaacaccc cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggacg cccacgcgaa acgccagacg guggucgugc ucggcucuca ggagggggcu     1380

guucauaccg cccuggccgg cgcucuugag gccgaaaugg auggagcuaa gggccggcug     1440

agcuccgggc accucaagug cagacugaaa auggacaaac uucgccugaa gggagugagc     1500

uauucucucu guacagccgc guucaccuuu acaaagaucc cggccgagac ccugcauggc     1560

acggucaccg uggaaguuca guacgcuggg acagacggac cuugcaaagu gcccgcccaa     1620

auggcugucg auaugcagac ccugacaccu gugggccggc ucauuaccgc caaccccgug     1680

aucacggagu ccacagaaaa cagcaagaug augcuggagc uugacccgcc uuucggggau     1740

ucuuacaucg uuauuggcgu cggagagaag aaaaucaccc accacuggca uagauccggc     1800

agcacaauug ggaaggcguu ugaagccacc gugcgcggag cuaaacggau ggccgugcug     1860

ggcgacaccg cuugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaagggc     1920

auccaccaga uuuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu     2040

ucccugaugu gucuugcgcu gggaggcgug cucaucuucc ugagcaccgc cguuucugcu     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4469
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4469
gggagaaagc uuaccaugaa gagacggggc gccgacacca gcguggggau cgucggacug       60

cuccugacaa cggcuauggc cgcggagguu acccgcagag gcuccgcuua cuauauguac      120

cuugaucgga acgacgccgg ggaagccauu ucuuucccca caacccuggg aaugaauaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg cuacaaugag cuacgagugc      240

ccuaugcugg augaaggggu ggagccggac gauguggacu guuggugcaa caccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aagcccgcag aucucggcgc      360

gcuguuaccc uucccagcca uuccacaaga aagcugcaaa cccggucuca gacauggcuc      420

gagagccgcg aguacaccaa acaccugauc agaguggaaa auuggauuuu ucggaacccu      480

ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaag      540

gucaucuauc uugugaugau ucugcucauc gcccccgcgu acuccauucg cugcaucgga      600

guuagcaaua gagauuuugu ggagggcaug ucugggggaa caugggucga cauugugcug      660

gaacauggcg ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa cauggcugag guccggagcu acugcuauga agccucuauu      780

uccgauaugg cuagcgacuc ccgcuguccc acgcaaggag aggccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagagaaca cucguugacc ggggcugggg caaugggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caaaaagaug      960

acaggaaaga gcauccagcc ugaaaaccuc gaguacagaa ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaaugau accggacaug aaacggacga gaaccgcgcg     1080

aaaguugaga ucacacccaa uucuccccgg gccgaagcua cccuuggcgg cuucgggagc     1140

cugggacucg auugugagcc uagaaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaacaaua agcacuggcu cgugcauaaa gaaugguucc acgacauucc ccugccuugg     1260

cacgccgggg ccgauaccgg aacaccccau uggaacaaua aggaggcucu ggucgaauuu     1320

aaagacgccc acgcgaagcg ccagacggug gucgugcucg gcucucagga gggggcuguu     1380

cauaccgccc uggccggcgc ucuugaggcc gaaauggaug gagcuaaagg ccggcugagc     1440

uccgggcacc ucaagugcag acugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucucucugua cagccgcguu caccuuuaca aaaaucccgg ccgagacccu gcauggcacg     1560

gucaccgugg aaguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug     1620

gcugucgaua ugcagacccu gacaccugug ggccggcuca uuaccgccaa ccccgugauc     1680

acggagucca cagaaaacag caaaaugaug cuggagcuug acccgccuuu cggggauucu     1740

uacaucguua uuggcgucgg agagaagaag aucacccacc acuggcauag auccggcagc     1800

acaauuggga aagcguuuga agccaccgug cgcggagcua agcggauggc cgugcugggc     1860

gacaccgcuu gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaca cgaaaaaugg gagcauuucc     2040

cugauguguc uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4470
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4470
gggagaaagc uuaccaugag acggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg cuauggccgc ggagguuacc cgcagaggcu ccgcuuacua uauguaccuu      120

gaucggaacg acgccgggga agccauuucu uuccccacaa cccugggaau gaauaagugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcua caaugagcua cgagugcccu      240

augcuggaug aaggggugga gccggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaaa aagggcgaag cccgcagauc ucggcgcgcu      360

guuacccuuc ccagccauuc cacaagaaaa cugcaaaccc ggucucagac auggcucgag      420

agccgcgagu acaccaagca ccugaucaga guggaaaauu ggauuuuucg gaacccuggc      480

uucgcccugg cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu      600

agcaauagag auuuugugga gggcaugucu gggggaacau gggucgacau ugugcuggaa      660

cauggcgggu gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggcugagguc cggagcuacu gcuaugaagc cucuauuucc      780

gauauggcua gcgacucccg cugucccacg caaggagagg ccuaccugga caagcagucu      840

gauacccagu augugugcaa aagaacacuc guugaccggg gcuggggcaa ugggugugga      900

cuguucggca aggggagccu ggugaccugc gccaaauuug cuugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga aaaccucgag uacagaauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaaa cggacgagaa ccgcgcgaag     1080

guugagauca cacccaauuc uccccgggcc gaagcuaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugagccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccccu gccuuggcac     1260

gccggggccg auaccggaac accccauugg aacaauaaag aggcucuggu cgaauuuaag     1320

gacgcccacg cgaaacgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau     1380

accgcccugg ccggcgcucu ugaggccgaa auggauggag cuaagggccg gcugagcucc     1440

gggcaccuca aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu     1500

cucuguacag ccgcguucac cuuuacaaag aucccggccg agacccugca uggcacgguc     1560

accguggaag uucaguacgc ugggacagac ggaccuugca aagugcccgc ccaaauggcu     1620

gucgauaugc agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca     1800

auugggaagg cguuugaagc caccgugcgc ggagcuaaac ggauggccgu gcugggcgac     1860

accgcuuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa gggcauccac     1920

cagauuuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4471
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4471
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga gguuacccgg cgcggcuccg cuuacuauau guaccuugau      120

agaaacgacg ccggggaagc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga gugcccuaug      240

cuggaugaag ggguggagcc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaagccc ggcgcucuag acgggcuguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgagagc      420

cgggaguaca ccaagcaccu gauccgcgug gaaaauugga uuuuuagaaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaggg caugucuggg ggaacauggg ucgacauugu gcuggaacau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaggucaga agcuacugcu augaagccuc uauuuccgau      780

auggcuagcg acucccggug ucccacgcaa ggagaggccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugaaaa ccucgaguac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugaaacgg acgagaacag agcgaagguu     1080

gagaucacac ccaauucucc ccgcgccgaa gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug agccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggaaugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaagagg cucuggucga auuuaaggac     1320

gcccacgcga aaagacagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga ggccgaaaug gauggagcua agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaagauc ccggccgaga cccugcaugg cacggucacc     1560

guggaaguuc aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg ccaaccccgu gaucacggag     1680

uccacagaaa acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggcgu uugaagccac cgugagagga gcuaaacgca uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4472
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4472
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaggu uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

aacgacgccg gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug      240

gaugaagggg uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caaaaagggc gaagccagac ggucucgcag agcuguuacc      360

cuucccagcc auuccacacg gaaacugcaa acccgcucuc agacauggcu cgagagcaga      420

gaguacacca agcaccugau ccggguggaa aauuggauuu uucgcaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauua gaugcaucgg aguuagcaau      600

cgggauuuug uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga gguccgcagc uacugcuaug aagccucuau uuccgauaug      780

gcuagcgacu ccagaugucc cacgcaagga gaggccuacc uggacaagca gucugauacc      840

caguaugugu gcaaacggac acucguugac cgcggcuggg gcaaugggug uggacuguuc      900

ggcaagggga gccuggugac cugcgccaaa uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gaaacggacg agaaccgggc gaagguugag     1080

aucacaccca auucucccag agccgaagcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugagc cucgcaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc     1320

cacgcgaaac ggcagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaggc cgaaauggau ggagcuaagg gcagacugag cuccgggcac     1440

cucaaaugcc gccugaagau ggacaaacuu cggcugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug     1560

gaaguucagu acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggcagacuc auuaccgcca accccgugau cacggagucc     1680

acagaaaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcauc gcuccggcag cacaauuggg     1800

aaggcguuug aagccaccgu gcggggagcu aaaagaaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu     1920

uucggagccg cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4473
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4473
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccugacaacg       60

gcuauggccg cggagguuac cagacgggga uccgcuuacu auauguaccu ugaucgcaac      120

gacgccggcg aagccauuuc uuuccccaca acccugggga ugaauaagug cuauauccag      180

auuauggauc ucggacacau gugugacgcu acaaugagcu acgagugccc uaugcuggau      240

gaaggcgugg agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uaugggacgu gucaucacaa aaagggagaa gccagacggu cucgcagagc uguuacccuu      360

cccagccauu ccacacggaa acugcaaacc cgcucucaga cauggcucga gagcagagag      420

uacaccaagc accugauccg gguggaaaau uggauuuuuc gcaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggc uccagcacgu cucagaaagu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauuagau gcaucggggu uagcaaucgg      600

gauuuugugg agggaauguc uggcgggaca ugggucgaca uugugcugga acauggaggc      660

ugugugaccg ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaggu ccgcagcuac ugcuaugaag ccucuauuuc cgauauggcu      780

agcgacucca gaugucccac gcaaggggag gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aacggacacu cguugaccgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucca agaaaaugac aggaaagagc      960

auccagccug aaaaccucga guacagaauc augcugucug uccacggguc ccaacacagc     1020

ggcaugauug ugaaugauac cgggcaugaa acggacgaga accgggcgaa gguugagauc     1080

acacccaauu cucccagagc cgaagcuacc cuuggaggcu ucggcagccu ggggcucgau     1140

ugugagccuc gcaccggacu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga augguuccac gacauucccc ugccuuggca cgccggcgcc     1260

gauaccggga caccccauug gaacaauaaa gaggcucugg ucgaauuuaa ggacgcccac     1320

gcgaaacggc agacgguggu cgugcucgga ucucaggagg gcgcuguuca uaccgcccug     1380

gccggggcuc uugaggccga aauggauggc gcuaagggaa gacugagcuc cggccaccuc     1440

aaaugccgcc ugaagaugga caagcugcgg cugaaagggg ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gagacccugc auggaacggu caccguggaa     1560

guucaguacg cuggcacaga cgggccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg aagacucauu accgccaacc ccgugaucac ggaguccaca     1680

gaaaacagca agaugaugcu ggagcuugac ccgccuuucg gcgauucuua caucguuauu     1740

ggggucggcg agaaaaagau cacccaccac uggcaucgcu ccggaagcac aauuggcaag     1800

gcguuugaag ccaccgugag aggggcuaaa cggauggccg ugcugggaga caccgcuugg     1860

gauuucggcu ccgucggggg agcccucaau ucucugggca aggggaucca ccagauuuuc     1920

ggcgccgcuu uuaaaagccu guucggaggc auguccuggu uuucucaaau ccuuaucggg     1980

acacuccuga uguggcuggg acucaacacg aagaauggca gcauuucccu gaugugucuu     2040

gcgcuugggg gagugcucau cuuccugagc accgccguuu cugcugacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4474
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4474
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu gacaacggcc       60

auggcugccg agguuaccag acggggaucc gcguacuaua uguaccuuga ucgcaacgac      120

gcuggcgaag ccauuucuuu ccccacaacc cuggggauga auaagugcua uauccagauu      180

auggaucucg gacacaugug ugacgccaca augagcuacg agugcccuau gcuggaugaa      240

ggcguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

gggacguguc aucacaaaaa gggagaagcu agacggucuc gcagagccgu uacccuuccc      360

agccauucca cacggaaacu gcaaacccgc ucucagacau ggcucgagag cagagaguac      420

accaagcacc ugauccgggu ggaaaauugg auuuuucgca acccuggcuu cgcucuggcc      480

gcggccgcua ucgccuggcu ccugggcucc agcacgucuc agaaagucau cuaucuugug      540

augauucugc ucaucgcucc cgccuacucc auuagaugca ucgggguuag caaucgggau      600

uuuguggagg gaaugucugg cgggacaugg gucgacauug ugcuggaaca uggaggcugu      660

gugaccguca uggcgcagga uaagccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg ccgagguccg cagcuacugc uaugaagcua gcauuuccga uauggccagc      780

gacuccagau gucccacgca aggggaggcu uaccuggaca agcagucuga uacccaguau      840

gugugcaaac ggacacucgu ugaccgcgga uggggcaaug gcugugggcu guucggaaag      900

ggcagccugg ugaccugcgc caaauuugcc ugcuccaaga aaaugacagg gaagucuauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggauccca acacagcggc     1020

augauuguga augauaccgg gcaugaaacg gacgagaacc gggcuaaggu ugagaucaca     1080

cccaauucuc ccagagcgga agccacccuu ggaggcuucg gcagccuggg gcucgauugu     1140

gagccucgca ccggacugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc uggcgccgau     1260

accgggacac cccauuggaa caauaaagag gcccuggucg aauuuaagga cgcucacgcc     1320

aaacggcaga cgguggucgu gcucggaucu caggagggcg cgguucauac cgcucuggcc     1380

ggggcccuug aggcugaaau ggauggcgcc aagggaagac ugagcuccgg ccaccucaaa     1440

ugccgccuga agauggacaa gcugcggcug aaagggguga gcuauucucu cuguacagcu     1500

gccuucaccu uuacaaagau cccggcggag acccugcaug gaacggucac cguggaaguu     1560

caguacgccg gcacagacgg gccuugcaaa gugcccgcuc aaauggccgu cgauaugcag     1620

acccugacac cugugggaag acucauuacc gcuaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucggcg auucuuacau cguuauuggg     1740

gucggcgaga aaaagaucac ccaccacugg caucgcuccg gaagcacaau uggcaaggcc     1800

uuugaagcga ccgugagagg ggccaaacgg auggcugugc ugggagacac cgccugggau     1860

uucggcuccg ucgggggagc ucucaauucu cugggcaagg ggauccacca gauuuucggc     1920

gccgccuuua aaagccuguu cggaggcaug uccugguuuu cucaaauccu uaucgggaca     1980

cuccugaugu ggcugggacu caacacgaag aauggcagca uuucccugau gugucuugcu     2040

cuugggggag ugcucaucuu ccugagcacc gcgguuucug ccgacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  4475
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4475
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccg auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4476
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4476
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgcgg uuaccagacg gggauccgcu uacuauaugu accuugaccg caacgaugcc      120

ggcgaggcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugcuacaaug agcuacgaau gcccuaugcu ggacgagggc      240

guggaaccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaggccaga cggucucgca gagcuguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgaaagcag agaguacacc      420

aagcaccuga uccgggugga gaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggaaggaa ugucuggcgg gacauggguc gauauugugc uggagcaugg aggcugugug      660

accgucaugg cccaggacaa gccuacaguu gauaucgaac uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaggccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaagccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauccag      960

ccugagaacc ucgaauacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugagacggau gaaaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaggc uacccuugga ggcuucggca gccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccccugccuu ggcacgccgg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaagcu cuggucgagu uuaaggaugc ccacgcgaaa     1320

cggcagacgg uggucgugcu cggaucucag gaaggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgagaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgaaacc cugcauggaa cggucaccgu ggagguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaggccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcug auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4477
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4477
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcggc uguuacccgg cgcggcuccg ccuacuauau guaccuugau      120

agaaacgacg ccggggaggc uauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgccacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaggcuc ggcgcucuag acgggccguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgaaagc      420

cgggaguaca ccaagcaccu gauccgcgug gagaauugga uuuuuagaaa cccuggcuuc      480

gcgcuggccg cugccgcuau cgccuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgcgccc gccuacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcucaggau aagccuacag uugacaucga acuugugacc      720

accacagugu ccaacauggc cgaggucaga agcuacugcu augaggcuuc uauuuccgau      780

auggccagcg acucccggug ucccacgcaa ggagaagccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcu aaauuugcgu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgaaaacag agccaagguu     1080

gagaucacac ccaauucucc ccgcgcugag gccacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggcugaua ccggaacacc ccauuggaac aauaaagaag cccuggucga guuuaaggac     1320

gcgcacgcua aaagacagac gguggucgug cucggcucuc aggaaggggc cguucauacc     1380

gcccuggcug gcgcccuuga ggcugagaug gauggagcca agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagcgg ccuucaccuu uacaaagauc ccggcugaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgccgg gacagacgga ccuugcaaag ugcccgcuca aauggccguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg cgaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggaa cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggccu uugaggcuac cgugagagga gccaaacgca uggcugugcu gggcgacacc     1860

gccugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag cugcguuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugccc ugggaggcgu gcucaucuuc cugagcaccg cuguuucugc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4478
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4478
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gaggcguuac gcggcgcggg      120

agcgccuacu auauguaccu ggauagaaac gacgcuggag aggccauuuc cuuucccacc      180

acacuuggca ugaauaaaug cuauauccaa auuauggauc uggggcauau gugugacgcg      240

accaugucuu acgaaugccc uaugcucgau gagggagugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggcaccu gucaccauaa gaaaggcgag      360

gcucggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg guuugcccuu gcugccgcug ccaucgcgug gcugcucgga      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ccccgcuuac      600

ucuauucggu gcaucggcgu uuccaaucgc gauuucgugg aagggaugag cggaggcacg      660

ugggucgaca uugugcucga gcacggggga ugugugacag ucauggccca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggcugaggu cagauccuac      780

ugcuaugagg ccagcauuuc cgauauggcg ucugacagcc ggugucccac acagggcgaa      840

gccuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggga auggaugugg ccuguuuggg aaaggaagcc uugugacaug cgcuaaguuc      960

gccugcucua aaaagaugac cggcaagucc auccaaccug agaaccugga auaccggauc     1020

augcucagcg uccacggguc ucagcauagc ggaaugauug ugaaugauac aggccacgag     1080

accgacgaaa acagagcuaa aguugagauc acgcccaauu ccccgcgcgc cgaggccaca     1140

cugggcgggu uuggaucucu uggccuggau ugugaaccuc ggaccgggcu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcuggagcg gauacaggca caccccacug gaacaauaag     1320

gaagcccuug ucgaguucaa agacgcucau gccaagagac agacaguggu cgugcugggg     1380

ucccaggaag gcgccguuca cacggcucuc gccggagcgc uggaggcuga gauggauggc     1440

gccaaagggc gccuuagcuc uggacaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggcg uguccuauag ccucuguacc gccgcuuuua cauucaccaa aaucccggcc     1560

gaaacacugc acgggaccgu cacgguggag guucaauacg cuggaaccga cggcccuugc     1620

aaggugcccg cccagauggc ggucgauaug cagacacuua ccccuguggg gcgccugauu     1680

acagccaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gcgacagcua caucguuauu ggagucggcg agaagaagau cacacaccau     1800

uggcaccggu ccgggucuac cauuggaaag gcuuucgagg ccacagugag aggcgcuaaa     1860

cgcauggccg ugcuggggga caccgcgugg gauuucggaa gcgucggcgg ggcccuuaau     1920

ucccucggca aaggaaucca ucaaauuuuu ggcgcugccu ucaagucucu guuuggggga     1980

augagcuggu ucucccagau ccugaucggc acccuccuga uguggcuugg gcugaacacc     2040

aaaaauggaa gcauuucucu caugugucug gcucugggcg gggugcuuau cuuucucucc     2100

acggccguua gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4479
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4479
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gagccguuac gcggcgcggc      120

agcgcuuacu auauguaccu ggauagaaac gacgccgggg aggcgauuuc cuuucccacc      180

acacuuggaa ugaauaaaug cuauauccaa auuauggauc ugggccauau gugugacgcu      240

accaugucuu acgaaugccc uaugcucgau gagggggugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggaaccu gucaccauaa gaaaggcgag      360

gcccggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg cuuugcucuu gccgcugccg cgaucgccug gcugcucggg      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ucccgccuac      600

ucuauucggu gcaucggagu uuccaaucgc gauuucgugg aaggcaugag cgggggaacg      660

ugggucgaca uugugcucga gcacggcggg ugugugacag ucauggcuca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggccgaggu cagauccuac      780

ugcuaugagg cgagcauuuc cgauauggcc ucugacagcc ggugucccac acagggagag      840

gcuuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggca augggugugg acuguuuggc aaagggagcc uugugacaug cgccaaguuc      960

gcuugcucua aaaagaugac cggaaagucc auccaaccug aaaaccugga auaccggauc     1020

augcucagcg uccacggcuc ucagcauagc gggaugauug ugaaugauac aggacacgag     1080

accgacgaaa acagagccaa aguugagauc acgcccaauu ccccgcgcgc cgaggcuaca     1140

cugggcggcu uugggucucu uggacuggau ugugaaccuc ggaccggccu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcgggggcc gauacaggaa caccccacug gaacaauaag     1320

gaagcucuug ucgaguucaa agacgcccau gccaagagac agacaguggu cgugcugggc     1380

ucccaggaag gggcuguuca cacggcccuc gcgggcgcuc uggaggccga gauggaugga     1440

gccaaaggcc gccuuagcuc ugggcaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggag uguccuauag ccucuguacc gcugccuuua cauucaccaa aaucccggcu     1560

gaaacacugc acggcaccgu cacgguggag guucaauacg ccgggaccga cggaccuugc     1620

aaggugcccg cgcagauggc cgucgauaug cagacacuua ccccuguggg ccgccugauu     1680

acagcuaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gggacagcua caucguuauu ggcgucggag agaagaagau cacacaccau     1800

uggcaccggu ccggcucuac cauugggaaa gccuucgagg cuacagugag aggagccaag     1860

cgcauggcgg ugcugggcga caccgccugg gauuucggga gcgucggagg cgcucuuaau     1920

ucccucggga aaggcaucca ucaaauuuuu ggagccgcuu ucaagucucu guuuggcggg     1980

augagcuggu ucucccagau ccugaucgga acccuccuga uguggcuugg ccugaacacc     2040

aaaaauggga gcauuucucu caugugucug gcccugggag gcgugcuuau cuuucucucc     2100

acggccguua gcgcugacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4480
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4480
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4481
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4481
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agcggcgcgg cgcugacacc       60

agcgugggga uugucggacu gcuccugaca acggccaugg cggcugaagu uaccagacgg      120

ggcuccgccu acuauaugua ccuugaucgc aaugacgccg gggaggcuau cucuuucccc      180

acaacccugg gaaugaacaa augcuauauu cagaucaugg aucucggcca caugugugac      240

gccacaauga gcuacgaaug cccuaugcug gaugaggggg uggaaccgga cgauguggac      300

uguuggugca auaccacauc caccuggguc guguauggaa cgugucauca caagaaaggc      360

gaggcuagac ggucucgcag agccguuacc cuucccagcc auuccacacg gaagcugcaa      420

acccgcucuc agacauggcu cgagagcaga gaauacacca agcaccugau ucggguggag      480

aacuggaucu uucgcaaucc uggcuucgcg cuggccgcug ccgcuaucgc cuggcuccug      540

ggguccagca cgucucagaa agucauuuau cuugugauga uccugcucau ugcgcccgcc      600

uacuccauca gaugcauugg aguuagcaac cgggauuuug uggaaggcau gucuggggga      660

acaugggucg acaucgugcu ggagcauggc ggguguguga ccgucauggc ucaggauaag      720

ccuacaguug acauugagcu ugugaccacc acagugucca auauggccga aguccgcagc      780

uacugcuaug aggcuucuau cuccgauaug gccagcgacu ccagaugucc cacgcaagga      840

gaagccuacc uggacaaaca gucugauacc caguaugugu gcaagcggac acucguugac      900

agaggcuggg gcaacgggug uggacuguuc ggcaaaggga gccuggugac cugcgcuaag      960

uuugcgugcu ccaagaaaau gacaggaaag agcauccagc cugagaaucu cgaauaccgc     1020

auuaugcugu cuguccacgg cucccaacac agcgggauga ucgugaacga uaccggacau     1080

gagacggacg agaaucgggc caaaguugaa auuacaccga acucucccag agcugaggcc     1140

acccuuggcg gcuucgggag ccugggacuc gauugugaac cucgcaccgg ccuggacuuu     1200

uccgaucuuu auuaccugac aaugaauaac aagcacuggc ucgugcauaa agagugguuc     1260

cacgacaucc cccugccuug gcacgccggg gcugauaccg gaacacccca uuggaauaac     1320

aaggaagccc uggucgaguu uaaagacgcg cacgcuaagc ggcagacggu ggucgugcuc     1380

ggcucucagg agggggccgu ucauaccgcc cuggcuggcg cccuugaagc ugagauggau     1440

ggagccaagg gcagacugag cuccgggcac cucaaaugcc gccugaagau ggacaaacuu     1500

cggcugaagg gagugagcua uucucucugu acagcggccu ucaccuuuac aaaaauuccg     1560

gcugaaaccc ugcauggcac ggucaccgug gagguucagu acgccgggac agacggaccu     1620

ugcaaggugc ccgcucaaau ggccgucgau augcagaccc ugacaccugu gggcagacuc     1680

aucaccgcga accccgugau cacggaaucc acagagaaua gcaagaugau gcuggagcuu     1740

gaucccccuu ucggggacuc uuacauuguu aucggcgucg gagagaaaaa gauuacccac     1800

cacuggcauc gcuccggcag cacaaucggg aaagccuuug aagcuaccgu gcggggagcc     1860

aagagaaugg cugugcuggg cgacaccgcc ugggauuucg gguccgucgg aggcgcccuc     1920

aacucucugg ggaaaggcau ucaccagauc uucggagcug cguuuaagag ccuguucggc     1980

gggauguccu gguuuucuca aauccuuauu ggaacacucc ugauguggcu gggccucaau     2040

acgaagaacg ggagcaucuc ccugaugugu cuugcccugg gaggcgugcu cauuuuccug     2100

agcaccgcug uuucugccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4482
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4482
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagcggcg cggcgcugac       60

accagcgugg ggaucgucgg acugcuccug acaacggcca uggcggcuga aguuaccaga      120

cggggcuccg ccuacuauau guaccuugau cgcaaugacg ccggggaggc uauuucuuuc      180

cccacaaccc ugggaaugaa caaaugcuau auccagauua uggaucucgg ccacaugugu      240

gacgccacaa ugagcuacga augcccuaug cuggaugagg ggguggaacc ggacgaugug      300

gacuguuggu gcaauaccac auccaccugg gucguguaug gaacguguca ucacaagaaa      360

ggcgaggcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      420

caaacccgcu cucagacaug gcucgagagc agagaauaca ccaagcaccu gauccgggug      480

gagaacugga ucuuucgcaa uccuggcuuc gcgcuggccg cugccgcuau ugccuggcuc      540

cuggggucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcgccc      600

gccuacucca uuagaugcau cggaguuagc aaccgggauu uuguggaagg caugucuggg      660

ggaacauggg ucgacauugu gcuggagcau ggcgggugug ugaccgucau ggcucaggau      720

aagccuacag uugacaucga gcuugugacc accacagugu ccaauauggc cgaaguccgc      780

agcuacugcu augaggcuuc uaucuccgau auggccagcg acuccagaug ucccacgcaa      840

ggagaagccu accuggacaa acagucugau acccaguaug ugugcaagcg gacacucguu      900

gacagaggcu ggggcaacgg guguggacug uucggcaaag ggagccuggu gaccugcgcu      960

aaguuugcgu gcuccaagaa aaugacagga aagagcauuc agccugagaa ucucgaauac     1020

cgcaucaugc ugucugucca cggcucccaa cacagcggga ugauugugaa cgauaccgga     1080

caugagacgg acgagaaucg ggccaaaguu gaaaucacac cgaacucucc cagagcugag     1140

gccacccuug gcggcuucgg gagccuggga cucgauugug aaccucgcac cggccuggac     1200

uuuuccgauc uuuauuaccu gacaaugaau aacaagcacu ggcucgugca uaaagagugg     1260

uuccacgaca uuccccugcc uuggcacgcc ggggcugaua ccggaacacc ccauuggaau     1320

aacaaggaag cccuggucga guuuaaagac gcgcacgcua agcggcagac gguggucgug     1380

cucggcucuc aggagggggc cguucauacc gcccuggcug gcgcccuuga agcugagaug     1440

gauggagcca agggcagacu gagcuccggg caccucaaau gccgccugaa gauggacaaa     1500

cuucggcuga agggagugag cuauucucuc uguacagcgg ccuucaccuu uacaaaaauc     1560

ccggcugaaa cccugcaugg cacggucacc guggagguuc aguacgccgg gacagacgga     1620

ccuugcaagg ugcccgcuca aauggccguc gauaugcaga cccugacacc ugugggcaga     1680

cucaucaccg cgaaccccgu gauuacggaa uccacagaga auagcaagau gaugcuggag     1740

cuugaucccc cuuucgggga cucuuacauc guuauuggcg ucggagagaa aaagaucacc     1800

caccacuggc aucgcuccgg cagcacaauu gggaaagccu uugaagcuac cgugcgggga     1860

gccaagagaa uggcugugcu gggcgacacc gccugggauu ucggguccgu cggaggcgcc     1920

cucaacucuc uggggaaagg cauccaccag aucuucggag cugcguuuaa gagccuguuc     1980

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacac uccugaugug gcugggccuc     2040

aauacgaaga acgggagcau uucccugaug ugucuugccc ugggaggcgu gcucaucuuc     2100

cugagcaccg cuguuucugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4483
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4483
gggagaaagc uuaccaugaa cgccagaaag gagaaaaagc ggcgcggcgc ugacaccagc       60

guggggaucg ucggacugcu ccugacaacg gccauggcgg cugaaguuac cagacggggc      120

uccgccuacu auauguaccu ugaucgcaau gacgccgggg aggcuauuuc uuuccccaca      180

acccugggaa ugaacaaaug cuauauccag auuauggauc ucggccacau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggggugg aaccggacga uguggacugu      300

uggugcaaua ccacauccac cugggucgug uauggaacgu gucaucacaa gaaaggcgag      360

gcuagacggu cucgcagagc cguuacccuu cccagccauu ccacacggaa gcugcaaacc      420

cgcucucaga cauggcucga gagcagagaa uacaccaagc accugauccg gguggagaac      480

uggauuuuuc gcaauccugg cuucgcgcug gccgcugccg cuaucgccug gcuccugggg      540

uccagcacgu cucagaaagu caucuaucuu gugaugauuc ugcucaucgc gcccgccuac      600

uccauuagau gcaucggagu uagcaaccgg gauuuugugg aaggcauguc ugggggaaca      660

ugggucgaca uugugcugga gcauggcggg ugugugaccg ucauggcuca ggauaagccu      720

acaguugaca ucgagcuugu gaccaccaca guguccaaua uggccgaagu ccgcagcuac      780

ugcuaugagg cuucuauuuc cgauauggcc agcgacucca gaugucccac gcaaggagaa      840

gccuaccugg acaaacaguc ugauacccag uaugugugca agcggacacu cguugacaga      900

ggcuggggca acgggugugg acuguucggc aaagggagcc uggugaccug cgcuaaguuu      960

gcgugcucca agaaaaugac aggaaagagc auccagccug agaaucucga auaccgcauc     1020

augcugucug uccacggcuc ccaacacagc gggaugauug ugaacgauac cggacaugag     1080

acggacgaga aucgggccaa aguugaaauc acaccgaacu cucccagagc ugaggccacc     1140

cuuggcggcu ucgggagccu gggacucgau ugugaaccuc gcaccggccu ggacuuuucc     1200

gaucuuuauu accugacaau gaauaacaag cacuggcucg ugcauaaaga gugguuccac     1260

gacauucccc ugccuuggca cgccggggcu gauaccggaa caccccauug gaauaacaag     1320

gaagcccugg ucgaguuuaa agacgcgcac gcuaagcggc agacgguggu cgugcucggc     1380

ucucaggagg gggccguuca uaccgcccug gcuggcgccc uugaagcuga gauggaugga     1440

gccaagggca gacugagcuc cgggcaccuc aaaugccgcc ugaagaugga caaacuucgg     1500

cugaagggag ugagcuauuc ucucuguaca gcggccuuca ccuuuacaaa aaucccggcu     1560

gaaacccugc auggcacggu caccguggag guucaguacg ccgggacaga cggaccuugc     1620

aaggugcccg cucaaauggc cgucgauaug cagacccuga caccuguggg cagacucauu     1680

accgcgaacc ccgugaucac ggaauccaca gagaauagca agaugaugcu ggagcuugau     1740

cccccuuucg gggacucuua caucguuauu ggcgucggag agaaaaagau cacccaccac     1800

uggcaucgcu ccggcagcac aauugggaaa gccuuugaag cuaccgugcg gggagccaag     1860

agaauggcug ugcugggcga caccgccugg gauuucgggu ccgucggagg cgcccucaac     1920

ucucugggga aaggcaucca ccagauuuuc ggagcugcgu uuaagagccu guucggcggg     1980

auguccuggu uuucucaaau ccuuaucgga acacuccuga uguggcuggg ccucaauacg     2040

aagaacggga gcauuucccu gaugugucuu gcccugggag gcgugcucau cuuccugagc     2100

accgcuguuu cugccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4484
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4484
gggagaaagc uuaccauggc cagaaaggag aaaaagcggc gcggcgcuga caccagcgug       60

gggaucgucg gacugcuccu gacaacggcc auggcggcug aaguuaccag acggggcucc      120

gccuacuaua uguaccuuga ucgcaacgac gccggggagg cuauuucuuu ccccacaacc      180

cugggaauga auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca      240

augagcuacg aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg      300

ugcaacacca cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu      360

agacggucuc gcagagccgu uacccuuccc agccauucca cacggaagcu gcaaacccgc      420

ucucagacau ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaauugg      480

auuuuucgca acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc      540

agcacgucuc agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc      600

auuagaugca ucggaguuag caaucgggau uuuguggaag gcaugucugg gggaacaugg      660

gucgacauug ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca      720

guugacaucg agcuugugac caccacagug uccaacaugg ccgaaguccg cagcuacugc      780

uaugaggcuu cuauuuccga uauggccagc gacuccagau gucccacgca aggagaagcc      840

uaccuggaca aacagucuga uacccaguau gugugcaagc ggacacucgu ugacagaggc      900

uggggcaaug gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg      960

ugcuccaaga aaaugacagg aaagagcauc cagccugaga accucgaaua ccgcaucaug     1020

cugucugucc acggcuccca acacagcggg augauuguga augauaccgg acaugagacg     1080

gacgagaacc gggccaaagu ugaaaucaca cccaauucuc ccagagcuga ggccacccuu     1140

ggcggcuucg ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau     1200

cuuuauuacc ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac     1260

auuccccugc cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa     1320

gcccuggucg aguuuaaaga cgcgcacgcu aagcggcaga cgguggucgu gcucggcucu     1380

caggaggggg ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc     1440

aagggcagac ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa     1560

acccugcaug gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag     1620

gugcccgcuc aaauggccgu cgauaugcag acccugacac cugugggcag acucauuacc     1680

gcgaaccccg ugaucacgga auccacagag aacagcaaga ugaugcugga gcuugacccg     1740

ccuuucgggg auucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaagcc uuugaagcua ccgugcgggg agccaagaga     1860

auggcugugc ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaauucu     1920

cuggggaaag gcauccacca gauuuucgga gcugcguuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag     2040

aaugggagca uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc     2100

gcuguuucug ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4485
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4485
gggagaaagc uuaccaugag aaaggagaaa aagcggcgcg gcgccgacac cagcgugggg       60

aucgucggac ugcuccugac aacggcuaug gccgcggaag uuaccagacg gggcuccgcu      120

uacuauaugu accuugaucg caacgacgcc ggggaggcca uuucuuuccc cacaacccug      180

ggaaugaaua aaugcuauau ccagauuaug gaucucggcc acauguguga cgcuacaaug      240

agcuacgaau gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc      300

aacaccacau ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggccaga      360

cggucucgca gagcuguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu      420

cagacauggc ucgagagcag agaauacacc aagcaccuga uccgggugga gaauuggauu      480

uuucgcaacc cuggcuucgc ccuggcggcc gcugccaucg cuuggcuccu gggguccagc      540

acgucucaga aagucaucua ucuugugaug auucugcuca ucgcccccgc guacuccauu      600

agaugcaucg gaguuagcaa ucgggauuuu guggaaggca ugucuggggg aacauggguc      660

gacauugugc uggagcaugg cgggugugug accgucaugg cccaggauaa gccuacaguu      720

gacaucgagc uugugaccac cacagugucc aacauggcug aaguccgcag cuacugcuau      780

gaggccucua uuuccgauau ggcuagcgac uccagauguc ccacgcaagg agaagccuac      840

cuggacaaac agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg      900

ggcaaugggu guggacuguu cggcaaaggg agccugguga ccugcgccaa guuugcuugc      960

uccaagaaaa ugacaggaaa gagcauccag ccugagaacc ucgaauaccg caucaugcug     1020

ucuguccacg gcucccaaca cagcgggaug auugugaaug auaccggaca ugagacggac     1080

gagaaccggg cgaaaguuga aaucacaccc aauucuccca gagccgaggc uacccuuggc     1140

ggcuucggga gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu     1200

uauuaccuga caaugaacaa uaagcacugg cucgugcaua aagagugguu ccacgacauu     1260

ccccugccuu ggcacgccgg ggccgauacc ggaacacccc auuggaacaa uaaggaagcu     1320

cuggucgagu uuaaagacgc ccacgcgaag cggcagacgg uggucgugcu cggcucucag     1380

gagggggcug uucauaccgc ccuggccggc gcucuugaag ccgagaugga uggagcuaag     1440

ggcagacuga gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag     1500

ggagugagcu auucucucug uacagccgcg uucaccuuua caaaaauccc ggccgaaacc     1560

cugcauggca cggucaccgu ggagguucag uacgcuggga cagacggacc uugcaaggug     1620

cccgcccaaa uggcugucga uaugcagacc cugacaccug ugggcagacu cauuaccgcc     1680

aaccccguga ucacggaauc cacagagaac agcaagauga ugcuggagcu ugacccgccu     1740

uucggggauu cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau     1800

cgcuccggca gcacaauugg gaaagcguuu gaagccaccg ugcggggagc uaagagaaug     1860

gccgugcugg gcgacaccgc uugggauuuc ggguccgucg gaggcgcccu caauucucug     1920

gggaaaggca uccaccagau uuucggagcc gcuuuuaaga gccuguucgg cgggaugucc     1980

ugguuuucuc aaauccuuau cggaacacuc cugauguggc ugggccucaa cacgaagaau     2040

gggagcauuu cccugaugug ucuugcgcug ggaggcgugc ucaucuuccu gagcaccgcc     2100

guuucugcuu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4486
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4486
gggagaaagc uuaccaugaa ggagaaaaag agacggggcg ccgacaccag cguggggauc       60

gucggacugc uccugacaac ggcuauggcc gcggaaguua cccgcagagg cuccgcuuac      120

uauauguacc uugaucggaa cgacgccggg gaggccauuu cuuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcccgcaga      360

ucucggcgcg cuguuacccu ucccagccau uccacaagaa agcugcaaac ccggucucag      420

acauggcucg agagccgcga auacaccaag caccugauca gaguggagaa uuggauuuuu      480

cggaacccug gcuucgcccu ggcggccgcu gccaucgcuu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cuccauucgc      600

ugcaucggag uuagcaauag agauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggccc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaac auggcugaag uccggagcua cugcuaugag      780

gccucuauuu ccgauauggc uagcgacucc cgcuguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg gggcuggggc      900

aaugggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaccucg aauacagaau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaaugaua ccggacauga gacggacgag     1080

aaccgcgcga aaguugaaau cacacccaau ucuccccggg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu agaaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagcgc cagacggugg ucgugcucgg cucucaggag     1380

ggggcuguuc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cggcugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccggcucau uaccgccaac     1680

cccgugauca cggaauccac agagaacagc aagaugaugc uggagcuuga cccgccuuuc     1740

ggggauucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcauaga     1800

uccggcagca caauugggaa agcguuugaa gccaccgugc gcggagcuaa gcggauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccgucggag gcgcccucaa uucucugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4487
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4487
gggagaaagc uuaccaugga gaagaaaaga cggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc uauggccgcg gaaguuaccc gcagaggcuc cgcuuacuau      120

auguaccuug aucggaacga cgccggggag gccauuucuu uccccacaac ccugggaaug      180

aauaagugcu auauccagau uauggaucuc ggccacaugu gugacgcuac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaacacc      300

acauccaccu gggucgugua uggaacgugu caucacaaaa agggcgaggc ccgcagaucu      360

cggcgcgcug uuacccuucc cagccauucc acaagaaaac ugcaaacccg gucucagaca      420

uggcucgaga gccgcgaaua caccaagcac cugaucagag uggagaauug gauuuuucgg      480

aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc uccugggguc cagcacgucu      540

cagaagguca ucuaucuugu gaugauucug cucaucgccc ccgcguacuc cauucgcugc      600

aucggaguua gcaauagaga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcccagg auaaaccuac aguugacauc      720

gagcuuguga ccaccacagu guccaacaug gcugaagucc ggagcuacug cuaugaggcc      780

ucuauuuccg auauggcuag cgacucccgc ugucccacgc aaggagaagc cuaccuggac      840

aagcagucug auacccagua ugugugcaaa agaacacucg uugaccgggg cuggggcaau      900

ggguguggac uguucggcaa ggggagccug gugaccugcg ccaaauuugc uugcuccaag      960

aagaugacag gaaaaagcau ccagccugag aaccucgaau acagaaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aaugauaccg gacaugagac ggacgagaac     1080

cgcgcgaagg uugaaaucac acccaauucu ccccgggccg aggcuacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccuaga accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga acaauaaaca cuggcucgug cauaaggagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggccga uaccggaaca ccccauugga acaauaaaga agcucugguc     1320

gaguuuaagg acgcccacgc gaaacgccag acgguggucg ugcucggcuc ucaggagggg     1380

gcuguucaua ccgcccuggc cggcgcucuu gaagccgaga uggauggagc uaagggccgg     1440

cugagcuccg ggcaccucaa gugcagacug aaaauggaca aacuucgccu gaagggagug     1500

agcuauucuc ucuguacagc cgcguucacc uuuacaaaga ucccggccga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcu gggacagacg gaccuugcaa agugcccgcc     1620

caaauggcug ucgauaugca gacccugaca ccugugggcc ggcucauuac cgccaacccc     1680

gugaucacgg aauccacaga gaacagcaag augaugcugg agcuugaccc gccuuucggg     1740

gauucuuaca ucguuauugg cgucggagaa aagaaaauca cccaccacug gcauagaucc     1800

ggcagcacaa uugggaaggc guuugaggcc accgugcgcg gagcuaaacg gauggccgug     1860

cugggcgaca ccgcuuggga uuucgggucc gucggaggcg cccucaauuc ucuggggaag     1920

ggcauccacc agauuuucgg agccgcuuuu aaaagccugu ucggcgggau guccugguuu     1980

ucucaaaucc uuaucggaac acuccugaug uggcugggcc ucaacacgaa gaaugggagc     2040

auuucccuga ugugucuugc gcugggaggc gugcucaucu uccugagcac cgccguuucu     2100

gcuugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4488
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4488
gggagaaagc uuaccaugaa gaaaagacgg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuga caacggcuau ggccgcggag guuacccgca gaggcuccgc uuacuauaug      120

uaccuugauc ggaacgacgc cggggaagcc auuucuuucc ccacaacccu gggaaugaau      180

aagugcuaua uccagauuau ggaucucggc cacaugugug acgcuacaau gagcuacgag      240

ugcccuaugc uggaugaagg gguggagccg gacgaugugg acuguuggug caacaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaaaaagg gcgaagcccg cagaucucgg      360

cgcgcuguua cccuucccag ccauuccaca agaaaacugc aaacccgguc ucagacaugg      420

cucgagagcc gcgaguacac caagcaccug aucagagugg aaaauuggau uuuucggaac      480

ccuggcuucg cccuggcggc cgcugccauc gcuuggcucc ugggguccag cacgucucag      540

aaggucaucu aucuugugau gauucugcuc aucgcccccg cguacuccau ucgcugcauc      600

ggaguuagca auagagauuu uguggagggc augucugggg gaacaugggu cgacauugug      660

cuggaacaug gcgggugugu gaccgucaug gcccaggaua aaccuacagu ugacaucgag      720

cuugugacca ccacaguguc caacauggcu gagguccgga gcuacugcua ugaagccucu      780

auuuccgaua uggcuagcga cucccgcugu cccacgcaag gagaggccua ccuggacaag      840

cagucugaua cccaguaugu gugcaaaaga acacucguug accggggcug gggcaauggg      900

uguggacugu ucggcaaggg gagccuggug accugcgcca aauuugcuug cuccaagaag      960

augacaggaa aaagcaucca gccugaaaac cucgaguaca gaaucaugcu gucuguccac     1020

ggcucccaac acagcgggau gauugugaau gauaccggac augaaacgga cgagaaccgc     1080

gcgaagguug agaucacacc caauucuccc cgggccgaag cuacccuugg cggcuucggg     1140

agccugggac ucgauuguga gccuagaacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaca auaaacacug gcucgugcau aaggaauggu uccacgacau uccccugccu     1260

uggcacgccg gggccgauac cggaacaccc cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggacg cccacgcgaa acgccagacg guggucgugc ucggcucuca ggagggggcu     1380

guucauaccg cccuggccgg cgcucuugag gccgaaaugg auggagcuaa gggccggcug     1440

agcuccgggc accucaagug cagacugaaa auggacaaac uucgccugaa gggagugagc     1500

uauucucucu guacagccgc guucaccuuu acaaagaucc cggccgagac ccugcauggc     1560

acggucaccg uggaaguuca guacgcuggg acagacggac cuugcaaagu gcccgcccaa     1620

auggcugucg auaugcagac ccugacaccu gugggccggc ucauuaccgc caaccccgug     1680

aucacggagu ccacagaaaa cagcaagaug augcuggagc uugacccgcc uuucggggau     1740

ucuuacaucg uuauuggcgu cggagagaag aaaaucaccc accacuggca uagauccggc     1800

agcacaauug ggaaggcguu ugaagccacc gugcgcggag cuaaacggau ggccgugcug     1860

ggcgacaccg cuugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaagggc     1920

auccaccaga uuuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu     2040

ucccugaugu gucuugcgcu gggaggcgug cucaucuucc ugagcaccgc cguuucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4489
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4489
gggagaaagc uuaccaugaa gagacggggc gccgacacca gcguggggau cgucggacug       60

cuccugacaa cggcuauggc cgcggagguu acccgcagag gcuccgcuua cuauauguac      120

cuugaucgga acgacgccgg ggaagccauu ucuuucccca caacccuggg aaugaauaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg cuacaaugag cuacgagugc      240

ccuaugcugg augaaggggu ggagccggac gauguggacu guuggugcaa caccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aagcccgcag aucucggcgc      360

gcuguuaccc uucccagcca uuccacaaga aagcugcaaa cccggucuca gacauggcuc      420

gagagccgcg aguacaccaa acaccugauc agaguggaaa auuggauuuu ucggaacccu      480

ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaag      540

gucaucuauc uugugaugau ucugcucauc gcccccgcgu acuccauucg cugcaucgga      600

guuagcaaua gagauuuugu ggagggcaug ucugggggaa caugggucga cauugugcug      660

gaacauggcg ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa cauggcugag guccggagcu acugcuauga agccucuauu      780

uccgauaugg cuagcgacuc ccgcuguccc acgcaaggag aggccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagagaaca cucguugacc ggggcugggg caaugggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caaaaagaug      960

acaggaaaga gcauccagcc ugaaaaccuc gaguacagaa ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaaugau accggacaug aaacggacga gaaccgcgcg     1080

aaaguugaga ucacacccaa uucuccccgg gccgaagcua cccuuggcgg cuucgggagc     1140

cugggacucg auugugagcc uagaaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaacaaua agcacuggcu cgugcauaaa gaaugguucc acgacauucc ccugccuugg     1260

cacgccgggg ccgauaccgg aacaccccau uggaacaaua aggaggcucu ggucgaauuu     1320

aaagacgccc acgcgaagcg ccagacggug gucgugcucg gcucucagga gggggcuguu     1380

cauaccgccc uggccggcgc ucuugaggcc gaaauggaug gagcuaaagg ccggcugagc     1440

uccgggcacc ucaagugcag acugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucucucugua cagccgcguu caccuuuaca aaaaucccgg ccgagacccu gcauggcacg     1560

gucaccgugg aaguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug     1620

gcugucgaua ugcagacccu gacaccugug ggccggcuca uuaccgccaa ccccgugauc     1680

acggagucca cagaaaacag caaaaugaug cuggagcuug acccgccuuu cggggauucu     1740

uacaucguua uuggcgucgg agagaagaag aucacccacc acuggcauag auccggcagc     1800

acaauuggga aagcguuuga agccaccgug cgcggagcua agcggauggc cgugcugggc     1860

gacaccgcuu gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaca cgaaaaaugg gagcauuucc     2040

cugauguguc uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4490
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4490
gggagaaagc uuaccaugag acggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg cuauggccgc ggagguuacc cgcagaggcu ccgcuuacua uauguaccuu      120

gaucggaacg acgccgggga agccauuucu uuccccacaa cccugggaau gaauaagugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcua caaugagcua cgagugcccu      240

augcuggaug aaggggugga gccggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaaa aagggcgaag cccgcagauc ucggcgcgcu      360

guuacccuuc ccagccauuc cacaagaaaa cugcaaaccc ggucucagac auggcucgag      420

agccgcgagu acaccaagca ccugaucaga guggaaaauu ggauuuuucg gaacccuggc      480

uucgcccugg cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu      600

agcaauagag auuuugugga gggcaugucu gggggaacau gggucgacau ugugcuggaa      660

cauggcgggu gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggcugagguc cggagcuacu gcuaugaagc cucuauuucc      780

gauauggcua gcgacucccg cugucccacg caaggagagg ccuaccugga caagcagucu      840

gauacccagu augugugcaa aagaacacuc guugaccggg gcuggggcaa ugggugugga      900

cuguucggca aggggagccu ggugaccugc gccaaauuug cuugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga aaaccucgag uacagaauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaaa cggacgagaa ccgcgcgaag     1080

guugagauca cacccaauuc uccccgggcc gaagcuaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugagccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccccu gccuuggcac     1260

gccggggccg auaccggaac accccauugg aacaauaaag aggcucuggu cgaauuuaag     1320

gacgcccacg cgaaacgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau     1380

accgcccugg ccggcgcucu ugaggccgaa auggauggag cuaagggccg gcugagcucc     1440

gggcaccuca aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu     1500

cucuguacag ccgcguucac cuuuacaaag aucccggccg agacccugca uggcacgguc     1560

accguggaag uucaguacgc ugggacagac ggaccuugca aagugcccgc ccaaauggcu     1620

gucgauaugc agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca     1800

auugggaagg cguuugaagc caccgugcgc ggagcuaaac ggauggccgu gcugggcgac     1860

accgcuuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa gggcauccac     1920

cagauuuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcuugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4491
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4491
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga gguuacccgg cgcggcuccg cuuacuauau guaccuugau      120

agaaacgacg ccggggaagc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga gugcccuaug      240

cuggaugaag ggguggagcc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaagccc ggcgcucuag acgggcuguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgagagc      420

cgggaguaca ccaagcaccu gauccgcgug gaaaauugga uuuuuagaaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaggg caugucuggg ggaacauggg ucgacauugu gcuggaacau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaggucaga agcuacugcu augaagccuc uauuuccgau      780

auggcuagcg acucccggug ucccacgcaa ggagaggccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugaaaa ccucgaguac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugaaacgg acgagaacag agcgaagguu     1080

gagaucacac ccaauucucc ccgcgccgaa gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug agccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggaaugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaagagg cucuggucga auuuaaggac     1320

gcccacgcga aaagacagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga ggccgaaaug gauggagcua agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaagauc ccggccgaga cccugcaugg cacggucacc     1560

guggaaguuc aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg ccaaccccgu gaucacggag     1680

uccacagaaa acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggcgu uugaagccac cgugagagga gcuaaacgca uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4492
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4492
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaggu uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

aacgacgccg gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug      240

gaugaagggg uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caaaaagggc gaagccagac ggucucgcag agcuguuacc      360

cuucccagcc auuccacacg gaaacugcaa acccgcucuc agacauggcu cgagagcaga      420

gaguacacca agcaccugau ccggguggaa aauuggauuu uucgcaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauua gaugcaucgg aguuagcaau      600

cgggauuuug uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga gguccgcagc uacugcuaug aagccucuau uuccgauaug      780

gcuagcgacu ccagaugucc cacgcaagga gaggccuacc uggacaagca gucugauacc      840

caguaugugu gcaaacggac acucguugac cgcggcuggg gcaaugggug uggacuguuc      900

ggcaagggga gccuggugac cugcgccaaa uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gaaacggacg agaaccgggc gaagguugag     1080

aucacaccca auucucccag agccgaagcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugagc cucgcaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc     1320

cacgcgaaac ggcagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaggc cgaaauggau ggagcuaagg gcagacugag cuccgggcac     1440

cucaaaugcc gccugaagau ggacaaacuu cggcugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug     1560

gaaguucagu acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggcagacuc auuaccgcca accccgugau cacggagucc     1680

acagaaaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcauc gcuccggcag cacaauuggg     1800

aaggcguuug aagccaccgu gcggggagcu aaaagaaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu     1920

uucggagccg cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4493
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4493
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccugacaacg       60

gcuauggccg cggagguuac cagacgggga uccgcuuacu auauguaccu ugaucgcaac      120

gacgccggcg aagccauuuc uuuccccaca acccugggga ugaauaagug cuauauccag      180

auuauggauc ucggacacau gugugacgcu acaaugagcu acgagugccc uaugcuggau      240

gaaggcgugg agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uaugggacgu gucaucacaa aaagggagaa gccagacggu cucgcagagc uguuacccuu      360

cccagccauu ccacacggaa acugcaaacc cgcucucaga cauggcucga gagcagagag      420

uacaccaagc accugauccg gguggaaaau uggauuuuuc gcaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggc uccagcacgu cucagaaagu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauuagau gcaucggggu uagcaaucgg      600

gauuuugugg agggaauguc uggcgggaca ugggucgaca uugugcugga acauggaggc      660

ugugugaccg ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaggu ccgcagcuac ugcuaugaag ccucuauuuc cgauauggcu      780

agcgacucca gaugucccac gcaaggggag gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aacggacacu cguugaccgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucca agaaaaugac aggaaagagc      960

auccagccug aaaaccucga guacagaauc augcugucug uccacggguc ccaacacagc     1020

ggcaugauug ugaaugauac cgggcaugaa acggacgaga accgggcgaa gguugagauc     1080

acacccaauu cucccagagc cgaagcuacc cuuggaggcu ucggcagccu ggggcucgau     1140

ugugagccuc gcaccggacu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga augguuccac gacauucccc ugccuuggca cgccggcgcc     1260

gauaccggga caccccauug gaacaauaaa gaggcucugg ucgaauuuaa ggacgcccac     1320

gcgaaacggc agacgguggu cgugcucgga ucucaggagg gcgcuguuca uaccgcccug     1380

gccggggcuc uugaggccga aauggauggc gcuaagggaa gacugagcuc cggccaccuc     1440

aaaugccgcc ugaagaugga caagcugcgg cugaaagggg ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gagacccugc auggaacggu caccguggaa     1560

guucaguacg cuggcacaga cgggccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg aagacucauu accgccaacc ccgugaucac ggaguccaca     1680

gaaaacagca agaugaugcu ggagcuugac ccgccuuucg gcgauucuua caucguuauu     1740

ggggucggcg agaaaaagau cacccaccac uggcaucgcu ccggaagcac aauuggcaag     1800

gcguuugaag ccaccgugag aggggcuaaa cggauggccg ugcugggaga caccgcuugg     1860

gauuucggcu ccgucggggg agcccucaau ucucugggca aggggaucca ccagauuuuc     1920

ggcgccgcuu uuaaaagccu guucggaggc auguccuggu uuucucaaau ccuuaucggg     1980

acacuccuga uguggcuggg acucaacacg aagaauggca gcauuucccu gaugugucuu     2040

gcgcuugggg gagugcucau cuuccugagc accgccguuu cugcuugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  4494
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4494
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu gacaacggcc       60

auggcugccg agguuaccag acggggaucc gcguacuaua uguaccuuga ucgcaacgac      120

gcuggcgaag ccauuucuuu ccccacaacc cuggggauga auaagugcua uauccagauu      180

auggaucucg gacacaugug ugacgccaca augagcuacg agugcccuau gcuggaugaa      240

ggcguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

gggacguguc aucacaaaaa gggagaagcu agacggucuc gcagagccgu uacccuuccc      360

agccauucca cacggaaacu gcaaacccgc ucucagacau ggcucgagag cagagaguac      420

accaagcacc ugauccgggu ggaaaauugg auuuuucgca acccuggcuu cgcucuggcc      480

gcggccgcua ucgccuggcu ccugggcucc agcacgucuc agaaagucau cuaucuugug      540

augauucugc ucaucgcucc cgccuacucc auuagaugca ucgggguuag caaucgggau      600

uuuguggagg gaaugucugg cgggacaugg gucgacauug ugcuggaaca uggaggcugu      660

gugaccguca uggcgcagga uaagccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg ccgagguccg cagcuacugc uaugaagcua gcauuuccga uauggccagc      780

gacuccagau gucccacgca aggggaggcu uaccuggaca agcagucuga uacccaguau      840

gugugcaaac ggacacucgu ugaccgcgga uggggcaaug gcugugggcu guucggaaag      900

ggcagccugg ugaccugcgc caaauuugcc ugcuccaaga aaaugacagg gaagucuauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggauccca acacagcggc     1020

augauuguga augauaccgg gcaugaaacg gacgagaacc gggcuaaggu ugagaucaca     1080

cccaauucuc ccagagcgga agccacccuu ggaggcuucg gcagccuggg gcucgauugu     1140

gagccucgca ccggacugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc uggcgccgau     1260

accgggacac cccauuggaa caauaaagag gcccuggucg aauuuaagga cgcucacgcc     1320

aaacggcaga cgguggucgu gcucggaucu caggagggcg cgguucauac cgcucuggcc     1380

ggggcccuug aggcugaaau ggauggcgcc aagggaagac ugagcuccgg ccaccucaaa     1440

ugccgccuga agauggacaa gcugcggcug aaagggguga gcuauucucu cuguacagcu     1500

gccuucaccu uuacaaagau cccggcggag acccugcaug gaacggucac cguggaaguu     1560

caguacgccg gcacagacgg gccuugcaaa gugcccgcuc aaauggccgu cgauaugcag     1620

acccugacac cugugggaag acucauuacc gcuaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucggcg auucuuacau cguuauuggg     1740

gucggcgaga aaaagaucac ccaccacugg caucgcuccg gaagcacaau uggcaaggcc     1800

uuugaagcga ccgugagagg ggccaaacgg auggcugugc ugggagacac cgccugggau     1860

uucggcuccg ucgggggagc ucucaauucu cugggcaagg ggauccacca gauuuucggc     1920

gccgccuuua aaagccuguu cggaggcaug uccugguuuu cucaaauccu uaucgggaca     1980

cuccugaugu ggcugggacu caacacgaag aauggcagca uuucccugau gugucuugcu     2040

cuugggggag ugcucaucuu ccugagcacc gcgguuucug ccugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4495
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  4495
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4496
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4496
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgcgg uuaccagacg gggauccgcu uacuauaugu accuugaccg caacgaugcc      120

ggcgaggcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugcuacaaug agcuacgaau gcccuaugcu ggacgagggc      240

guggaaccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaggccaga cggucucgca gagcuguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgaaagcag agaguacacc      420

aagcaccuga uccgggugga gaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggaaggaa ugucuggcgg gacauggguc gauauugugc uggagcaugg aggcugugug      660

accgucaugg cccaggacaa gccuacaguu gauaucgaac uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaggccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaagccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauccag      960

ccugagaacc ucgaauacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugagacggau gaaaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaggc uacccuugga ggcuucggca gccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccccugccuu ggcacgccgg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaagcu cuggucgagu uuaaggaugc ccacgcgaaa     1320

cggcagacgg uggucgugcu cggaucucag gaaggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgagaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgaaacc cugcauggaa cggucaccgu ggagguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaggccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4497
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4497
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcggc uguuacccgg cgcggcuccg ccuacuauau guaccuugau      120

agaaacgacg ccggggaggc uauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgccacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaggcuc ggcgcucuag acgggccguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgaaagc      420

cgggaguaca ccaagcaccu gauccgcgug gagaauugga uuuuuagaaa cccuggcuuc      480

gcgcuggccg cugccgcuau cgccuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgcgccc gccuacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcucaggau aagccuacag uugacaucga acuugugacc      720

accacagugu ccaacauggc cgaggucaga agcuacugcu augaggcuuc uauuuccgau      780

auggccagcg acucccggug ucccacgcaa ggagaagccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcu aaauuugcgu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgaaaacag agccaagguu     1080

gagaucacac ccaauucucc ccgcgcugag gccacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggcugaua ccggaacacc ccauuggaac aauaaagaag cccuggucga guuuaaggac     1320

gcgcacgcua aaagacagac gguggucgug cucggcucuc aggaaggggc cguucauacc     1380

gcccuggcug gcgcccuuga ggcugagaug gauggagcca agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagcgg ccuucaccuu uacaaagauc ccggcugaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgccgg gacagacgga ccuugcaaag ugcccgcuca aauggccguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg cgaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggaa cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggccu uugaggcuac cgugagagga gccaaacgca uggcugugcu gggcgacacc     1860

gccugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag cugcguuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugccc ugggaggcgu gcucaucuuc cugagcaccg cuguuucugc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4498
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4498
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gaggcguuac gcggcgcggg      120

agcgccuacu auauguaccu ggauagaaac gacgcuggag aggccauuuc cuuucccacc      180

acacuuggca ugaauaaaug cuauauccaa auuauggauc uggggcauau gugugacgcg      240

accaugucuu acgaaugccc uaugcucgau gagggagugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggcaccu gucaccauaa gaaaggcgag      360

gcucggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg guuugcccuu gcugccgcug ccaucgcgug gcugcucgga      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ccccgcuuac      600

ucuauucggu gcaucggcgu uuccaaucgc gauuucgugg aagggaugag cggaggcacg      660

ugggucgaca uugugcucga gcacggggga ugugugacag ucauggccca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggcugaggu cagauccuac      780

ugcuaugagg ccagcauuuc cgauauggcg ucugacagcc ggugucccac acagggcgaa      840

gccuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggga auggaugugg ccuguuuggg aaaggaagcc uugugacaug cgcuaaguuc      960

gccugcucua aaaagaugac cggcaagucc auccaaccug agaaccugga auaccggauc     1020

augcucagcg uccacggguc ucagcauagc ggaaugauug ugaaugauac aggccacgag     1080

accgacgaaa acagagcuaa aguugagauc acgcccaauu ccccgcgcgc cgaggccaca     1140

cugggcgggu uuggaucucu uggccuggau ugugaaccuc ggaccgggcu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcuggagcg gauacaggca caccccacug gaacaauaag     1320

gaagcccuug ucgaguucaa agacgcucau gccaagagac agacaguggu cgugcugggg     1380

ucccaggaag gcgccguuca cacggcucuc gccggagcgc uggaggcuga gauggauggc     1440

gccaaagggc gccuuagcuc uggacaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggcg uguccuauag ccucuguacc gccgcuuuua cauucaccaa aaucccggcc     1560

gaaacacugc acgggaccgu cacgguggag guucaauacg cuggaaccga cggcccuugc     1620

aaggugcccg cccagauggc ggucgauaug cagacacuua ccccuguggg gcgccugauu     1680

acagccaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gcgacagcua caucguuauu ggagucggcg agaagaagau cacacaccau     1800

uggcaccggu ccgggucuac cauuggaaag gcuuucgagg ccacagugag aggcgcuaaa     1860

cgcauggccg ugcuggggga caccgcgugg gauuucggaa gcgucggcgg ggcccuuaau     1920

ucccucggca aaggaaucca ucaaauuuuu ggcgcugccu ucaagucucu guuuggggga     1980

augagcuggu ucucccagau ccugaucggc acccuccuga uguggcuugg gcugaacacc     2040

aaaaauggaa gcauuucucu caugugucug gcucugggcg gggugcuuau cuuucucucc     2100

acggccguua gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4499
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4499
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gagccguuac gcggcgcggc      120

agcgcuuacu auauguaccu ggauagaaac gacgccgggg aggcgauuuc cuuucccacc      180

acacuuggaa ugaauaaaug cuauauccaa auuauggauc ugggccauau gugugacgcu      240

accaugucuu acgaaugccc uaugcucgau gagggggugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggaaccu gucaccauaa gaaaggcgag      360

gcccggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg cuuugcucuu gccgcugccg cgaucgccug gcugcucggg      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ucccgccuac      600

ucuauucggu gcaucggagu uuccaaucgc gauuucgugg aaggcaugag cgggggaacg      660

ugggucgaca uugugcucga gcacggcggg ugugugacag ucauggcuca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggccgaggu cagauccuac      780

ugcuaugagg cgagcauuuc cgauauggcc ucugacagcc ggugucccac acagggagag      840

gcuuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggca augggugugg acuguuuggc aaagggagcc uugugacaug cgccaaguuc      960

gcuugcucua aaaagaugac cggaaagucc auccaaccug aaaaccugga auaccggauc     1020

augcucagcg uccacggcuc ucagcauagc gggaugauug ugaaugauac aggacacgag     1080

accgacgaaa acagagccaa aguugagauc acgcccaauu ccccgcgcgc cgaggcuaca     1140

cugggcggcu uugggucucu uggacuggau ugugaaccuc ggaccggccu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcgggggcc gauacaggaa caccccacug gaacaauaag     1320

gaagcucuug ucgaguucaa agacgcccau gccaagagac agacaguggu cgugcugggc     1380

ucccaggaag gggcuguuca cacggcccuc gcgggcgcuc uggaggccga gauggaugga     1440

gccaaaggcc gccuuagcuc ugggcaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggag uguccuauag ccucuguacc gcugccuuua cauucaccaa aaucccggcu     1560

gaaacacugc acggcaccgu cacgguggag guucaauacg ccgggaccga cggaccuugc     1620

aaggugcccg cgcagauggc cgucgauaug cagacacuua ccccuguggg ccgccugauu     1680

acagcuaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gggacagcua caucguuauu ggcgucggag agaagaagau cacacaccau     1800

uggcaccggu ccggcucuac cauugggaaa gccuucgagg cuacagugag aggagccaag     1860

cgcauggcgg ugcugggcga caccgccugg gauuucggga gcgucggagg cgcucuuaau     1920

ucccucggga aaggcaucca ucaaauuuuu ggagccgcuu ucaagucucu guuuggcggg     1980

augagcuggu ucucccagau ccugaucgga acccuccuga uguggcuugg ccugaacacc     2040

aaaaauggga gcauuucucu caugugucug gcccugggag gcgugcuuau cuuucucucc     2100

acggccguua gcgcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4500
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 1 opt

<400>  4500
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4501
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 1 opt

<400>  4501
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaca      240

ugugacgcca ccaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacacuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaucucagac cuggcucgag agccgggaau acacgaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacauc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcucag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacc      840

caaggagaag ccuaccugga caaacagucu gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcuaaguuug cgugcuccaa gaaaaugacc ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauacg     1080

ggacaugaga cagacgagaa uagagccaaa guggaaauua ccccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auacaggaac gccccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acagcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacgcugca uggcaccgug acaguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cggcgaaccc cguuaucaca gaauccaccg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac acagccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaaugcca agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca ccgccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4502
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 1 opt

<400>  4502
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgg auacgaaacg     1080

gaugaggaca gagcuaaggu ggaggucaca ccuaauagcc cgcgcgcgga agccacccuu     1140

ggcggcuucg ggucccuggg acucgauugu gagccccgga ccggccugga cuuuucugau     1200

cuuuauuacc ugacaaugaa caauaaacac uggcucgugc auaaggaaug guuccacgac     1260

auuccucugc ccuggcacgc uggggccgau accggaacac cucauuggaa caauaaagag     1320

gcccuggucg aauuuaagga cgcucacgcc aagagacaga cggugguugu gcucggcagc     1380

caggaggggg cgguccauac cgcucuggcc ggcgcccuug aggcugaaau ggauggagcc     1440

aaaggccgcc uguucuccgg gcaccucaag ugccggcuga aaauggacaa acuuagacug     1500

aagggaguga gcuauucucu cuguacagcu gccuuuaccu ucacaaaggu ucccgcggag     1560

acccugcaug gcacggugac cgucgaagug caguacgccg ggacagacgg accgugcaag     1620

aucccugugc aaauggcugu ugauaugcag acccugacac ccgucggccg ccucaucacc     1680

gccaacccug ugauuacgga guccacagaa aauagcaaaa ugaugcugga gcuugacccu     1740

cccuuugggg auucuuacau cgugauuggc gucggagaca agaaaaucac ccaccacugg     1800

caucgguccg gcagcacaau ugggaaggcu uucgaggcca ccgugagagg agcgaaacgc     1860

auggccguuc ugggcgauac cgcuugggac uucggguccg ugggaggcgu cuuuaacucu     1920

cucgggaagg gcauccacca gaucuucgga gccgcuuuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauucu gaucggaaca cuucucgugu ggcugggccu gaacacgaaa     2040

aaugggagca uuucccucac cugucuggcc cuuggaggcg uuaugaucuu ccugagcaca     2100

gccgugucug cugaugucgg gugcuccgug gacuuuagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4503
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 1 opt

<400>  4503
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4504
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 1 opt

<400>  4504
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgu gaaugauacg     1080

ggauacgaaa cagacgagaa cagagcuaag gucgagguga ccccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaaac acuggcucgu ccauaaggaa     1260

ugguuccacg acauuccucu gcccuggcac gcuggggccg auacaggaac gccucauugg     1320

aacaauaaag aggcccuggu ggaauuuaag gacgcucacg ccaagagaca gaccguugug     1380

guccucggca gccaggaggg ggcggugcau acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggcaa gcuguucucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuucggc ugaagggagu uagcuauucu cucuguaccg cugccuuuac auucaccaag     1560

gugcccgcgg agacgcugca uggcaccguc acaguggaag ugcaguacgc cgggaccgac     1620

ggaccgugca aaaucccugu ucaaauggcu gucgauaugc agacacugac ccccgugggc     1680

agacucauca cggccaaccc ugugauuaca gaguccaccg aaaacagcaa gaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgucauug gcgugggaga caaaaagauc     1800

acacaccacu ggcaucgcuc cggcagcacc auugggaagg cuuucgaggc caccguucgg     1860

ggagcgaaaa gaauggccgu gcugggcgau acagcuuggg acuucggguc cgucggaggc     1920

guguuuaauu cucucgggaa gggcauccac cagaucuucg gagccgcuuu uaaaagccug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cgcuucucgu uuggcugggc     2040

cugaacacca agaaugggag cauuucccuc acaugucugg cccuuggagg cgugaugauc     2100

uuccugagca ccgccgucuc ugcugaugug gggugcuccg uugacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4505
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 1 opt

<400>  4505
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucacgcugg ucgccguggc uaucgccugg      540

cuccugggga gcucuacauc ccagaagguu auuuaccuug ugaugauccu gcucauugcu      600

ccugccuaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaaccu ggguggacgu cguucuggaa cacggcgggu gugugacagu gauggcgcag      720

gauaaaccca ccgucgacau cgagcuugug accacaacgg uuagcaacau ggccgaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacc      840

caaggagagg cuuaucugga caagcagagc gauacacagu acgucugcaa aagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuacaugc      960

gccaaguuua ccugcagcaa aaagaugacg ggaaaaucua uucagcccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc caacauucug ggaugauugu caaugaugaa     1080

aacagagcca agguggaggu cacaccuaau agcccgcgcg cugaggcgac ccuuggaggc     1140

uucggcuccc uggggcucga cugugaaccc cggaccggac uggauuuuuc ugaccuuuac     1200

uaucugacaa ugaacaauaa acacuggcuc gugcauaagg agugguucca cgauaucccu     1260

cugcccuggc acgccggcgc ugacaccggg acaccucauu ggaacaauaa agaagcccug     1320

guugaguuua aggaugccca cgcuaagaga cagacggugg ucgugcucgg aagccaggaa     1380

ggcgccguuc auaccgcgcu ggcuggggcc cuugaggccg agauggacgg cgcuaaagga     1440

cgccuguucu ccggccaccu caagugccgg cugaaaaugg auaaacuuag acugaagggg     1500

gugagcuacu cucucuguac agccgcuuuu accuucacaa agguccccgc cgaaacccug     1560

cauggaacgg ugaccgugga gguucaguau gcgggcacag acgggccgug caaggucccu     1620

gcccaaaugg cuguggacau gcagacccug acacccgugg gacgccucau uaccgccaac     1680

ccugucauca cggaauccac agagaacagc aaaaugaugc uggaacuuga uccucccuuu     1740

ggcgacucuu acaucgugau ugggguuggc gauaagaaaa ucacccacca cuggcaucgg     1800

uccggaagca caauuggcaa ggcuuucgag gccaccguga gaggggcgaa acgcauggcc     1860

guccugggag acaccgcuug ggauuucggc uccguugggg gaguguuuaa uucucucggc     1920

aaggggaucc accagauuuu cggcgccgcu uuuaagagcc uguucggagg cauguccugg     1980

uuuucucaaa uccugaucgg gacacuucuc guguggcugg gacugaacac gaaaaauggc     2040

agcauuuccc ucaccugucu ggcccuuggg ggagucauga ucuuccugag cacagccgug     2100

ucugcugacg uuggcugcuc cguggauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4506
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 1 opt

<400>  4506
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccgcaag      360

aaaggcgaag ccagacggag ccgcagagcg gugacccuuc cgucccauuc uacacggaag      420

cugcaaaccc gcagccagac auggcucgag uccagagagu auaccaagca ccugauuaaa      480

guugaaaauu ggaucuuucg gaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucgcug caucggagug ucuaauagag auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cauggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

cggucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucuag auguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa acgcacacuc      900

gucgaccggg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uauagaauua ugcuguccgu ucacggcagc caacacucug ggaugaucgu gaaugauacg     1080

ggauacgaaa cagacgagaa ccgcgcuaag gucgagguga ccccuaauag cccgcgggcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagcccag aacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaaac auuggcucgu ccacaaggaa     1260

ugguuccaug acauuccucu gcccuggcac gcuggggccg auacaggaac gccucacugg     1320

aacaauaaag aggcccuggu ggaauuuaag gacgcucaug ccaagcgcca gaccguugug     1380

guccucggca gccaggaggg ggcggugcac acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggcaa gcuguucucc gggcaucuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu uagcuauucu cucuguaccg cugccuuuac auucaccaag     1560

gugcccgcgg agacgcugca cggcaccguc acaguggaag ugcaguacgc cgggaccgac     1620

ggaccgugca aaaucccugu ucaaauggcu gucgauaugc agacacugac ccccgugggc     1680

cgccucauca cggccaaccc ugugauuaca gaguccaccg aaaacagcaa gaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgucauug gcgugggaga caaaaagauc     1800

acacaucacu ggcaccgguc cggcagcacc auugggaagg cuuucgaggc caccguuaga     1860

ggagcgaaac gcauggccgu gcugggcgau acagcuuggg acuucggguc cgucggaggc     1920

guguuuaauu cucucgggaa gggcauccau cagaucuucg gagccgcuuu uaaaagccug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cgcuucucgu uuggcugggc     2040

cugaacacca agaaugggag cauuucccuc acaugucugg cccuuggagg cgugaugauc     2100

uuccugagca ccgccgucuc ugcugaugug gggugcuccg uugacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4507
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 1 opt

<400>  4507
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugacggu gaaugauauc     1080

ggauacgaaa cagacgagaa cagagcuaag gucgagguga ccccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaaac acuggcucgu ccauaaggaa     1260

ugguuccacg acauuccucu gcccuggcac gcuggggccg auacaggaac gccucauugg     1320

aacaauaaag aggcccuggu ggaauuuaag gacgcucacg ccaagagaca gaccguugug     1380

guccucggca gccaggaggg ggcggugcau acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggcaa gcuguucucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuucggc ugaagggagu uagcuauucu cucuguaccg cugccuuuac auucaccaag     1560

gugcccgcgg agacgcugca uggcaccguc acaguggaag ugcaguacgc cgggaccgac     1620

ggaccgugca aaaucccugu ucaaauggcu gucgauaugc agacacugac ccccgugggc     1680

agacucauca cggccaaccc ugugauuaca gaguccaccg aaaacagcaa gaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgucauug gcgugggaga caaaaagauc     1800

acacaccacu ggcaucgcuc cggcagcacc auugggaagg cuuucgaggc caccguucgg     1860

ggagcgaaaa gaauggccgu gcugggcgau acagcuuggg acuucggguc cgucggaggc     1920

guguuuaauu cucucgggaa gggcauccac cagaucuucg gagccgcuuu uaaaagccug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cgcuucucgu uuggcugggc     2040

cugaacacca agaaugggag cauuucccuc acaugucugg cccuuggagg cgugaugauc     2100

uuccugagca ccgccgucuc ugcugaugug gggugcuccg uugacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4508
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 1 opt

<400>  4508
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgg auacgaaacg     1080

gaugagaaua gagcuaaggu ggaggucaca ccuaacagcc cgcgcgcgga agccacccuu     1140

ggcggcuucg ggucccuggg acucgacugu gagccccgga ccggccugga uuuuucugac     1200

cuuuauuacc ugacaaugaa uaacaaacac uggcucgugc auaaggaaug guuccacgau     1260

auuccucugc ccuggcacgc uggggccgac accggaacac cucauuggaa uaacaaagag     1320

gcccuggucg aauuuaagga ugcucacgcc aagagacaga cggugguugu gcucggcagc     1380

caggaggggg cgguccauac cgcucuggcc ggcgcccuug aggcugaaau ggacggagcc     1440

aaaggcaagc uguucuccgg gcaccucaaa ugccgccuga agauggauaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcu gccuuuaccu ucacaaaggu ucccgcggag     1560

acccugcaug gcacggugac cgucgaagug caguacgccg ggacagacgg accgugcaaa     1620

aucccugugc aaauggcugu ugacaugcag acccugacac ccgucggcag acucaucacc     1680

gccaauccug ugauuacgga guccacagaa aacagcaaga ugaugcugga gcuugauccu     1740

cccuuugggg acucuuacau cgugauuggc gucggagaua aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaggcu uucgaggcca ccgugcgggg agcgaaaaga     1860

auggccguuc ugggcgacac cgcuugggau uucggguccg ugggaggcgu cuuuaacucu     1920

cucgggaagg gcauccacca gaucuucgga gccgcuuuua aaagccuguu cggcgggaug     1980

uccugguuuu cucaaauucu gaucggaaca cuucucgugu ggcugggccu gaauacgaag     2040

aacgggagca uuucccucac cugucuggcc cuuggaggcg uuaugaucuu ccugagcaca     2100

gccgugucug cugacgucgg gugcuccgug gauuuuagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4509
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 1 opt

<400>  4509
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggcg gggcauucac cagaucuucg gagcugcguu uaaaagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4510
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 1 opt

<400>  4510
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacgccagcg uggggaucgu cggacugcuc cugaccacag cgauggcugc cgaaguuacg      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgcugggga ggccauuucu      180

uuccccacca cacugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccaca ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcuc uagacgggcg guuacgcuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaucucagac cuggcucgag agccgggaau acacaaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcccugg cugccgcugc cauugcgugg      540

cuccuggggu ccagcaccuc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcuuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacgu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug acaaccaccg ucuccaauau ggcugaagug      780

agaagcuacu gcuaugaggc cucuaucucc gauauggcca gcgacucccg gugucccaca      840

caaggagagg cuuaccugga caaacagucu gauacgcagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcgaaguuug ccugcuccaa gaaaaugacc ggaaagagca uccagccuga aaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauaca     1080

ggacaugaga ccgacgagaa uagagcuaaa guggaaauua cgccgaacuc uccccgcgcc     1140

gaggccacac uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gcuggggccg auacaggaac accccauugg     1320

aauaacaagg aagcgcuggu ggaguuuaaa gacgcucacg ccaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acggcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg ccgcuuucac auuuaccaaa     1560

auuccggccg aaacacugca uggcaccgug acgguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcg guggauaugc agacacugac cccugugggc     1680

cgccucauca cagccaaccc cguuaucacc gaauccacgg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcucucaacu cucuggggaa aggcauucac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaauacaa agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca cggccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4511
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 1 opt

<400>  4511
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc cuggcgcucg ccgcugccgc uauugccugg      540

cugcuugggu ccagcacguc ucagaaaguc aucuaucugg ugaugauucu ccugaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuucgugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuugag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuggug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccucga caaacagucu gauacccagu auguuugcaa gagaacacug      900

guggaccgcg gcuggggcaa cgggugugga cuguuuggca aagggagccu cgugaccugc      960

gcuaaguucg cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucuggaa     1020

uaccggauua ugcuuucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc ugggcggcuu ugggagccuc ggacuggauu gugaaccucg gaccggccuu     1200

gacuucuccg aucuguauua ccucacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuucacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccucgu ggaguucaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucugggcu cucaggaggg ggccgugcau accgcccuug cuggcgcccu ggaagcugag     1440

auggauggag ccaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggac     1500

aaacugagac ucaagggagu cagcuauucu cuguguacag cggccuuuac cuucacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccucac accugugggc     1680

cgccugauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggauc ccccuuuugg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuucgaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccucggcgac accgccuggg auuuuggguc cgugggaggc     1920

gcccugaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu caagagccuu     1980

uuuggcggga uguccugguu cucucaaauc cucauuggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag caucucccuu augugucugg cccucggagg cguucugauu     2100

uuucugagca ccgcuguguc ugccgacguc gggugcuccg uggauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4512
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 1 opt

<400>  4512
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu gguaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucau cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4513
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 1 opt

<400>  4513
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaaauc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4514
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 1 opt

<400>  4514
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccc augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccuggc ucaaauggcc guggauaugc agacccucac acccgugggc     1680

cgccugauca ccgcgaaccc uguuaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggacc cucccuucgg ggauucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccucggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccugaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccuu     1980

uucggcggga uguccugguu uucucaaauc cucauuggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag caucucccuu augugucugg cccucggagg cguucugauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4515
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 1 opt

<400>  4515
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu acccuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacacuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaucucagac cuggcucgag agccgggaau acacgaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacauc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcucag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacc      840

caaggagaag ccuaccugga caaacagucu gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcuaaguuug cgugcuccaa gaaaaugacc ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauacg     1080

ggacaugaga cagacgagaa uagagccaaa guggaaauua ccccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auacaggaac gccccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acagcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacgcugca uggcaccgug acaguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cggcgaaccc cguuaucaca gaauccaccg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac acagccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaauacca agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca cagcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4516
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 1 opt

<400>  4516
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccguggucuc ugcugacgug gggugcuccg uugauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4517
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 1 opt

<400>  4517
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccgcu ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcuc uagacgggcg guuacgcuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaccucagac cuggcucgag ucucgggaau acacaaagca ccugauccgc      480

guggagaacu ggaucuuuag aaaucccggc uucgcccugg cugccgcugc cauugcgugg      540

cuccugggga gcuccaccag ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

ccugcuuacu cuauucggug caucggaguu uccaaccgcg auuuugugga aggcaugagc      660

gggggaacgu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccca ccguggacau ugagcuugug acaaccaccg ucucuaauau ggcugaagug      780

agauccuacu gcuaugaggc cagcaucucu gauauggccu ccgacagccg guguccuaca      840

caaggagagg cuuaccugga caaacagucc gauacgcagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggucucu ggucacaugc      960

gcgaaguuug ccugcagcaa gaaaaugacc ggaaagucca uccagccgga gaaucucgaa     1020

uaccggauua ugcugagcgu gcacggcucu caacacuccg ggaugaucgu uaacgauaca     1080

ggacaugaga ccgacgagaa uagagcuaaa guggaaauua cgcccaacag cccucgcgcc     1140

gaggccacac uuggcggcuu cgggucucug ggacucgauu gugaaccccg gaccggccug     1200

gacuuuagcg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccucu gcccuggcac gcuggggccg auacaggaac cccgcauugg     1320

aauaacaagg aagcgcuggu ggaguuuaaa gacgcucacg ccaagagaca gacagucgug     1380

guucucggcu cccaggaggg ggccgugcau acggcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguaccg ccgcuuucac auuuaccaaa     1560

auuccugccg aaacacugca uggcaccgug acgguugagg ugcaguacgc ugggaccgac     1620

ggacccugca aggucccugc ccaaauggcg guggauaugc agacacugac ccccgugggc     1680

cgccucauca cagccaaccc cguuaucacc gaaucuacgg aaaauuccaa gaugaugcug     1740

gagcuugacc cuccguucgg ggauagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc uggcuccacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcucucaacu cccuggggaa aggcauucac cagaucuucg gagcggccuu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaauacaa agaacggguc uaucagccug augugucuug cucugggagg cguucucauu     2100

uuccugucca cggccgugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4518
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 1 opt

<400>  4518
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc uaaaaagguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaaaccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

cagggagaag ccuaccugga caagcaaucu gauacccagu auguuugcaa aagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aggggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa aaagaugaca ggaaaaagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc cagcacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaag guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaaac acuggcucgu ccauaaggag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaaag aagcccuggu ggaguuuaag gacgcgcacg cuaagagaca aacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaaaggccg ccugagcucc gggcaccuca agugccggcu gaaaauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaag     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucagauggcc guggauaugc aaacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa aaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga aaagaaaauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaagg ccuuugaggc uaccgugaga     1860

ggagccaaac gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa gggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucagauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga aaaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4519
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 1 opt

<400>  4519
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacgcg gcagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cuauucgcug caucggaguu uccaacagag auuuugugga aggcaugagc      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucucuaauau ggccgaagug      780

cgguccuacu gcuaugaggc uagcaucucu gauauggccu ccgacagcag augucccacg      840

caaggagaag ccuaccugga caaacagucc gauacccagu auguuugcaa gcgcacacuc      900

guggaccggg gcuggggcaa cgggugugga cuguucggca aagggucucu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggaaagucca uccagccuga gaaucucgaa     1020

uacagaauua ugcugagcgu ccacggcucu caacacuccg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa ucgcgccaaa guggaaauua caccgaacag cccccgggcu     1140

gaggccaccc uuggcggcuu cgggucucug ggacucgauu gugaaccuag aaccggccug     1200

gacuuuagcg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagcgcca gacggucgug     1380

guucucggcu cccaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg gcugucuagc gggcaccuca aaugcagacu gaagauggac     1500

aaacuucgcc ugaagggagu cuccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cggcucauca ccgcgaaccc cguuaucacg gaaucuacag aaaauuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcauagauc uggcuccaca aucgggaaag ccuuugaggc uaccgugcgc     1860

ggagccaagc ggauggcugu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcccucaacu cccuggggaa aggcauucac cagaucuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacggguc uaucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucca ccgcugugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4520
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 1 opt

<400>  4520
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaagcgcu       60

gacaccuccg ugggcaucgu cgggcugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggau cugccuacua uauguaccuu gauagaaaug acgccggcga ggcuauuagc      180

uuccccacaa cccuggggau gaacaaaugc uauauccaga uuauggaucu cggacacaug      240

ugugacgcca caauguccua cgaaugcccu augcuggaug agggcgugga accggacgau      300

guggacuguu ggugcaauac cacaucuacc ugggucgugu augggacgug ucaucacaag      360

aaaggagagg cucggcgcag cagacgggcc guuacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggca gcucuacguc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuaca gcauucggug caucgggguu ucuaaccgcg auuuugugga aggaaugucc      660

ggcgggacau gggucgacgu ggugcuggag cauggaggcu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucagcaauau ggccgaagug      780

agaucuuacu gcuaugaggc uuccaucagc gauauggccu ccgacucucg gugucccacg      840

caaggggaag ccuaccugga caaacagagc gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gauggggcaa cggcuguggg cuguucggaa aaggcucccu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca gggaagucua uccagccuga gaaucucgaa     1020

uaccggauua ugcuguccgu gcacggaagc caacacucug gcaugaucgu uaacgauacc     1080

gggcaugaga cggacgagaa uagagccaaa guggaaauua caccgaacag cccccgcgcu     1140

gaggccaccc uuggaggcuu cggcucccug gggcucgauu gugaaccucg gaccggacug     1200

gacuuuucug aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggcgcug auaccgggac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggaa gccaggaggg cgccgugcau accgcccugg cuggggcccu ugaagcugag     1440

auggauggcg ccaagggacg ccuguccagc ggccaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggggu cucuuauucc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggaacggug accguugagg ugcaguacgc cggcacagac     1620

gggccuugca agguccccgc ucaaauggcc guggauaccc agacacugac cccuguggga     1680

cgccucauca cggcgaaccc cguuaucaca gaaagcaccg aaaauucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg cgacuccuac auugucaucg gggugggcga gaaaaagauu     1800

acacaccacu ggcaucggag cggauccacc aucggcaaag ccuuugaggc uaccgugaga     1860

ggggccaagc gcauggcugu ccugggagac acagccuggg auuucggcuc ugucggggga     1920

gcccucaaca gccugggcaa agggauucac cagaucuucg gcgcugcguu uaagucccug     1980

uucggaggca ugucuugguu uagccaaauc cuuauuggga cgcuccugau guggcuggga     2040

cucaauacca agaacggcuc caucagccug augugucuug cccucggggg aguucugauu     2100

uuccugucua cagcuguguc cgccgacguc ggcugcagcg uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4521
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 1 opt

<400>  4521
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccaaug uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggca gcgccuacua uauguaccuu gauagaaacg acgccgggga ggcuauuucc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugucuua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac cacaagcacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc cagacgggcc guuacccuuc ccucucauag cacacgcaag      420

cugcaaacca gaucccagac auggcucgag ucucgggaau acaccaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggga gcuccacgag ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cuauucggug caucggaguu uccaaucgcg auuuugugga aggcaugagc      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucucuaacau ggccgaagug      780

agauccuacu gcuaugaggc uagcaucucu gauauggccu ccgacagccg gugucccacg      840

caaggagaag ccuaccugga caaacagucc gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucucu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggaaagucca uccagccuga gaaccucgaa     1020

uaccggauua ugcugagcgu ccacggcucu caacacuccg ggaugaucgu uaaugauacc     1080

ggacaugaga cggacgagaa cagagccaaa guggaaauua caccgaauag cccccgcgcu     1140

gaggccaccc uuggcggcuu cgggucucug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuagcg aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aacaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cccaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaucc cguuaucacg gaaucuacag agaacuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc uggcuccaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagcccgcc ggauggcugu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcccucaauu cccuggggaa gggcauucac cagaucuucg gagcugcguu uaaaucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaacacga agaauggguc uaucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucca ccgcugugag cgccgacguc gggugcucug uggauuuuuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4522
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 1 opt

<400>  4522
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gaaaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugagguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gacagaaaug augccgggga agcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaccu cggccacaug      240

ugugaugcca caaugagcua cgagugcccu augcuggacg aaggggugga gccggaugac      300

guggauuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgaa agccgggagu acaccaagca ccugauccgc      480

guggaaaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg acuuugugga gggcaugucu      660

gggggaacau gggucgaugu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gacaagccua caguggauau ugaacuugug accaccacag ucuccaauau ggccgaggug      780

agaagcuacu gcuaugaggc uucuaucucc gacauggcca gcgauucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gacacccagu auguuugcaa gagaacacuc      900

guggaucgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga aaaucucgag     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgacacc     1080

ggacaugaga cggaugaaaa uagagccaaa guggagauua caccgaacuc uccccgcgcu     1140

gaagccaccc uuggcggcuu cgggagccug ggacucgacu gugagccucg gaccggccug     1200

gauuuuuccg accuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagaa     1260

ugguuccacg auaucccccu gccuuggcac gccggggcug acaccggaac accccauugg     1320

aauaacaagg aggcccuggu ggaguuuaaa gaugcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaagg ggccgugcau accgcccugg cuggcgcccu ugaggcugaa     1440

auggacggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggau     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug agacccugca uggcacggug accguugaag ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggacaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaguccacag agaauagcaa gaugaugcug     1740

gaacuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgau accgccuggg acuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgauguc gggugcuccg uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4523
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 1 opt

<400>  4523
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cccugcggug cauuggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuugag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau cgagcuggug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuauuucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccucga caaacagucu gauacccagu auguuugcaa gagaacacug      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu cgugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucuggaa     1020

uaccggauca ugcuuucugu ccacggcucc caacacagcg ggaugauugu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc ugggcggcuu cgggagccuc ggacuggauu gugaaccucg gaccggccuu     1200

gacuuuuccg aucuguauua ccucacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccucgu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucugggcu cucaggaggg ggccgugcau accgcccuug cuggcgcccu ggaagcugag     1440

auggauggag ccaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggac     1500

aaacugagac ucaagggagu cagcuauucu cuguguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccucac accugugggc     1680

cgccugauua ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggauc ccccuuucgg ggacucuuac aucgucauug gcgugggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccucggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccugaacu cucuggggaa aggcauccac cagauuuucg gagcugcguu uaagagccuu     1980

uucggcggga uguccugguu uucucaaauc cucaucggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag cauuucccuu augugucugg cccucggagg cguucugauc     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4524
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 1 opt

<400>  4524
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccaaug uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggca gcgccuacua uauguaccuu gauagaaacg acgccgggga ggcuauuucc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugucuua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac cacaagcacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc cagacgggcc guuacccuuc ccucucauag cacacgcaag      420

cugcaaacca gaucccagac auggcucgag ucucgggaau acaccaagca ccugauccgc      480

guggagaauu ggauguuuag aaacccuggc uucgcgcugg ccgcugccgc uaucgccugg      540

cuccugggga gcuccacgag ccagaaaguc auuuaucuug ugaugauccu gcucauugcg      600

cccgccuacu cuauccggug cauuggaguu uccaaucgcg auuuugugga aggcaugagc      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau cgagcuugug accaccacag ucucuaacau ggccgaagug      780

agauccuacu gcuaugaggc uagcauuucu gauauggccu ccgacagccg gugucccacg      840

caaggagaag ccuaccugga caaacagucc gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucucu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggaaagucca uccagccuga gaaccucgaa     1020

uaccggauca ugcugagcgu ccacggcucu caacacuccg ggaugauugu uaaugauacc     1080

ggacaugaga cggacgagaa cagagccaaa guggaaauca caccgaauag cccccgcgcu     1140

gaggccaccc uuggcggcuu cgggucucug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuagcg aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aacaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cccaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauua ccgcgaaucc cguuaucacg gaaucuacag agaacuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac aucgucauug gcgugggaga gaaaaagauc     1800

acccaccacu ggcaucgguc uggcuccaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcccucaauu cccuggggaa aggcauccac cagauuuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuaucggaa cacuccugau guggcugggc     2040

cucaacacga agaauggguc uauuagccug augugucuug cccugggagg cguucucauc     2100

uuccugucca ccgcugugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4525
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 1 opt

<400>  4525
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggauccugag aaauccuggc uuugcgcucg ccgcugccgc uauugccugg      540

cugcuugggu ccagcacguc ucagaaaguc aucuaucugg ugaugauucu ccugaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuucgugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuugag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuggug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccucga caaacagucu gauacccagu auguuugcaa gagaacacug      900

guggaccgcg gcuggggcaa cgggugugga cuguuuggca aagggagccu cgugaccugc      960

gcuaaguucg cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucuggaa     1020

uaccggauua ugcuuucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc ugggcggcuu ugggagccuc ggacuggauu gugaaccucg gaccggccuu     1200

gacuucuccg aucuguauua ccucacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuucacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccucgu ggaguucaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucugggcu cucaggaggg ggccgugcau accgcccuug cuggcgcccu ggaagcugag     1440

auggauggag ccaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggac     1500

aaacugagac ucaagggagu cagcuauucu cuguguacag cggccuuuac cuucacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccucac accugugggc     1680

cgccugauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggauc ccccuuuugg ggacucuuac auugucaucg gcauuggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuucgaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcucggcgac accgccuggg auuuuggguc cgucggaggc     1920

gcccugaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu caagagccuu     1980

uuuggcggga uguccugguu cucucaaauu cucaucggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag cauuucccuu augugucugg cccucggagg cgugcugauc     2100

uuucugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4526
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 1 opt

<400>  4526
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug uccaccaccg ucagcaauau ggccgaagug      780

agaucuuacu gcuaugaggc uacaaucucc gauaucgcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggccguc     1380

gugcucggcu cucaggaggg ggccguucau accgcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag ccgcuuucac cuuuacaaaa     1560

aucccggccg aaacccugca uggcacgguc accguggagg uucaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcg gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgccaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaagg cuuuugaagc caccgugaga     1860

ggagcuaaac gcauggccgu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcucucaacu cucuggggaa aggcauccac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cucugggagg cgugcucauc     2100

uuccugagca ccgccguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4527
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 1 opt

<400>  4527
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggca cccaggaggg ggccgugcau acagcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacgcugca uggcaccgug acaguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cggcgaaccc cguuaucaca gaaucuaccg aaaauuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc uggcuccacc aucgggaaag ccuuugaggc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac acagccuggg auuucgggag cgugggaggc     1920

gcccucaacu cccuggggaa aggcauucac cagaucuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaauacca agaacggguc uaucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucca cagcugugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4528
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 1 opt

<400>  4528
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagc ugcaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccucac accugugggc     1680

cgccugauca ccgcgaaccc cgugaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggauc ccccuuucgg ggacucuuac auuguuaucg gcgucggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcucggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccugaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccuu     1980

uucggcggga uguccugguu uucucaaauc cucauuggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag caucucccuu augugucugg cccucggagg cgugcugauu     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4529
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 1 opt

<400>  4529
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gaaaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugagguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gacagaaaug augccgggga agcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaccu cggccacaug      240

ugugaugcca caaugagcua cgagugcccu augcuggacg aaggggugga gccggaugac      300

guggauuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgaa agccgggagu acaccaagca ccugauccgc      480

aucgaaaacu ggauuuuuag aaauccuggc uucgcgcugg ccgcugccgc uaucgccugg      540

cuccuggggu ccagcacguc ucagaaagug auuuaucuug ucaugauccu gcucauugcg      600

cccgccuacu ccauccggug cauuggagug agcaaccgcg acuuuguuga gggcaugucu      660

gggggaacau ggguggaugu cgugcuggag cauggcgggu gugugaccgu cauggcucag      720

gacaagccua caguugauau cgaacuugug accaccacag uguccaauau ggccgagguc      780

agaagcuacu gcuaugaggc uucuaucucc gacauggcca gcgauucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gacacccagu augugugcaa gagaacacuc      900

guugaucgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga aaaucucgag     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgacacc     1080

ggacaugaga cggaugaaaa uagagccaaa guugagauca caccgaacuc uccccgcgcu     1140

gaagccaccc uuggcggcuu cgggagccug ggacucgacu gugagccucg gaccggccug     1200

gauuuuuccg accuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagaa     1260

ugguuccacg auauuccccu gccuuggcac gccggggcug acaccggaac accccauugg     1320

aauaacaagg aggcccuggu cgaguuuaaa gaugcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaagg ggccguucau accgcccugg cuggcgcccu ugaggcugaa     1440

auggacggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggau     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug agacccugca uggcacgguc accguggaag uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgacaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaguccacag agaauagcaa gaugaugcug     1740

gaacuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgau accgccuggg acuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgaugug gggugcuccg ucgacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4530
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 1 opt

<400>  4530
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccauug agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucgucauug gcgugggaga gaaaaagauc     1800

acacaccacu ggcaucgguc cggcagcacc auugggaaag ccuuugaagc uacagugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cccuccugau guggcugggc     2040

cucaauacaa agaacgggag cauuucccug augugucuug cccugggagg cguucucauc     2100

uuccugagca cggcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4531
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 1 opt

<400>  4531
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ucaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag ccgcguucac cuuuacaaaa     1560

auuccggccg aaacccugca uggcacgguu accguggagg uccaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu guggauaugc agacccugac accuguuggc     1680

cgccucauca ccgccaaccc cgucaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugugaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag cguuugaagc caccgucaga     1860

ggagcuaagc gcauggccgu gcugggcgac accgcuuggg auuucggguc cguuggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagccgcuuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucuccuuc augugucugg cgcuuggagg cgugcugauu     2100

uuucucagca ccgccgucuc ugcugacgug gggugcuccg uugauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4532
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 1 opt

<400>  4532
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggcaa acugagcucc gggcaccuca agugccgccu gaaaauggac     1500

aaacuucggc ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaag     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

agacucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa aaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga aaagaaaauu     1800

acccaccacu ggcaucgcuc cggcagcaca aucgggaagg ccuuugaggc uaccgugcgg     1860

ggagccaaaa gaauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa gggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga aaaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4533
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 1 opt

<400>  4533
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gaggcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaagg ggccgugcau accgcccugg cuggcgcccu ugaggcugaa     1440

auggacggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggau     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug agacccugca uggcacggug accguugaag ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggacaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaguccacag agaauagcaa gaugaugcug     1740

gaacuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgau accgccuggg acuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgauguc gggugcuccg uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4534
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 1 opt

<400>  4534
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cggggcugac     1620

ggaccuugca agguccccgc ccaaauggcg guggauaugc agacacugac cccugugggc     1680

cgccucauca cagccaaccc cguuaucacc gaauccacgg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcucucaacu cucuggggaa aggcauucac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaauacaa agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca cggccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4535
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 1 opt

<400>  4535
gggagaaagc uuaccaugag aaucauuaac gccggcaagg agaaaaagcg gcgcggggcu       60

gacaccaaug ugggaaucgu cggccugcuc cugacaacgg ccauggcggc ugaaguuacc      120

agacggggga gcgccuacua uauguaccuu gaucgcaacg acgccggaga ggcuauuucc      180

uuccccacaa cccugggcau gaauaaaugc uauauccaga uuauggaucu cgggcacaug      240

ugugacgcca caaugucuua cgaaugcccu augcuggaug agggagugga accggacgau      300

guggacuguu ggugcaacac cacaagcacc ugggucgugu auggcacgug ucaucacaag      360

aaaggcgagg cuagacgguc ccgcagagcc guuacccuuc ccucucauag cacacggaag      420

cugcaaaccc gcucccagac auggcucgag ucuagagaau acaccaagca ccugauccgg      480

guggagaauu ggaucuuucg caacccuggg uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggaa gcuccacgag ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cuauuagaug caucggcguu uccaaucggg auuuugugga agggaugagc      660

ggaggcacau gggucgacgu ggugcuggag caugggggau gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucucuaacau ggccgaagug      780

cgcuccuacu gcuaugaggc uagcaucucu gauauggccu ccgacagcag augucccacg      840

caaggcgaag ccuaccugga caaacagucc gauacccagu auguuugcaa gcggacacuc      900

guggacagag gcugggggaa uggauguggc cuguucggga aaggaucucu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggcaagucca uccagccuga gaaccucgaa     1020

uaccgcauua ugcugagcgu ccacgggucu caacacuccg gaaugaucgu uaaugauacc     1080

ggccaugaga cggacgagaa ccgggccaaa guggaaauua caccgaauag ccccagagcu     1140

gaggccaccc uuggcggguu cggaucucug ggccucgauu gugaaccucg caccgggcug     1200

gacuuuagcg aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggagcug auaccggcac accccauugg     1320

aacaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagcggca gacggucgug     1380

guucucgggu cccaggaggg cgccgugcau accgcccugg cuggagcccu ugaagcugag     1440

auggauggcg ccaaggggag acugucuagc ggacaccuca aaugccgccu gaagauggac     1500

aaacuucggc ugaagggcgu cuccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca ugggacggug accguugagg ugcaguacgc cggaacagac     1620

ggcccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggg     1680

agacucauca ccgcgaaucc cguuaucacg gaaucuacag agaacuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg cgacagcuac auugucaucg gagugggcga gaaaaagauu     1800

acccaccacu ggcaucgcuc uggguccaca aucggaaaag ccuuugaagc uaccgugcgg     1860

ggcgccagac gcauggcugu ccugggggac accgccuggg auuucggaag cgugggcggg     1920

gcccucaauu cccugggcaa gggcauucac cagaucuucg gagcugcguu uaaaucucug     1980

uucgggggaa ugagcugguu uucccaaauc cuuauuggca cacuccugau guggcugggg     2040

cucaacacga agaauggauc uaucagccug augugucuug cccugggcgg gguucucauu     2100

uuccugucca ccgcugugag cgccgacguc ggcugcucug uggauuuuuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4536
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 1 opt

<400>  4536
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gaaaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugagguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gacagaaaag augccgggga agcuauuucu      180

uuccccacaa cccugggaau gaauaagugc uauauccaga uuauggaccu cggccacaug      240

ugugaugcca caaugagcua cgagugcccu augcuggacg aaggggugga gccggaugac      300

guggauuguu ggugcaacac cacauccacc ugggucgugu auggaacgug ucaucacaaa      360

aagggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgaa agccgggagu acaccaaaca ccugauccgc      480

guggaaaauu ggaucuuuag aaacccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaagguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaucgcg acuuugugga gggcaugucu      660

gggggaacau gggucgaugu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gacaaaccua caguggauau ugaacuugug accaccacag ucuccaacau ggccgaggug      780

agaagcuacu gcuaugaggc uucuaucucc gacauggcca gcgauucccg gugucccacg      840

caaggagaag ccuaccugga caagcagucu gacacccagu auguuugcaa aagaacacuc      900

guggaucgcg gcuggggcaa ugggugugga cuguucggca aggggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa aaagaugaca ggaaaaagca uccagccuga aaaccucgag     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaaugacacc     1080

ggacaugaga cggaugaaaa cagagccaag guggagauua cacccaauuc uccgcgcgcu     1140

gaagccaccc uuggcggcuu cgggagccug ggacucgacu gugagccucg gaccggccug     1200

gauuuuuccg accuuuauua ccugacaaug aacaauaaac acuggcucgu ccauaaggaa     1260

ugguuccacg auaucccccu gccuuggcac gccggggcug acaccggaac accccauugg     1320

aacaauaaag aggcccuggu ggaguuuaag gaugcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaagg ggccgugcau accgcccugg cuggcgcccu ugaggcugaa     1440

auggacggag ccaaaggccg ccugagcucc gggcaccuca agugccggcu gaaaauggau     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaag     1560

auuccggcug agacccugca uggcacggug accguugaag ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggacaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaguccacag agaacagcaa aaugaugcug     1740

gaacuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaagaaaauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaagg ccuuugaagc uaccgugaga     1860

ggagccaaac gcauggcugu ccugggcgau accgccuggg acuucggguc cgugggaggc     1920

gcccucaauu cucuggggaa gggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaacacga aaaaugggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgauguc gggugcuccg uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4537
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 1 opt

<400>  4537
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauaugguua gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

gucgaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gccaaguuug cuugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ucacggcucc caacacagcg ggaugaucgu gaacgauacc     1080

ggacaugaga cggacgagaa uagagcgaaa gucgaaauua caccgaacuc uccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auaccggaac accccauugg     1320

aauaacaagg aagcucuggu cgaguuuaaa gacgcccacg cgaagagaca gacggugguu     1380

gugcucggcu cucaggaggg ggcuguccau accgcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag ccgcguucac cuuuacaaaa     1560

auuccggccg aaacccugca uggcacgguu accguggagg uccaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu guggauaugc agacccugac accuguuggc     1680

cgccucauca ccgccaaccc cgucaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugugaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag cguuugaagc caccgucaga     1860

ggagcuaagc gcauggccgu gcugggcgac accgcuuggg auuucggguc cguuggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagccgcuuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cgcugggagg cgugcucauu     2100

uuccugagca ccgccgucuc ugcugacgug gggugcuccg uugauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4538
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 1 opt

<400>  4538
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccaaug uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggca gcgccuacua uauguaccuu gauagaaacg acgccgggga ggcuauuucc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugucuua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacau gaccagcaca ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc cagacgggcc guuacgcuuc ccucucauag cacccgcaag      420

cugcaaacaa gaucccagac cuggcucgag ucucgggaau acacaaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggga gcuccaccag ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cuauucggug caucggaguu uccaaucgcg auuuugugga aggcaugagc      660

gggggaacgu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcucag      720

gauaagccua ccguggacau ugagcuugug acaaccaccg ucucuaacau ggccgaagug      780

agauccuacu gcuaugaggc uagcaucucu gauauggccu ccgacagccg gugucccaca      840

caaggagaag ccuaccugga caaacagucc gauacgcagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucucu ggucacaugc      960

gcuaaguuug cgugcagcaa gaaaaugacc ggaaagucca uccagccuga gaaccucgaa     1020

uaccggauua ugcugagcgu gcacggcucu caacacuccg ggaugaucgu uaaugauaca     1080

ggacaugaga ccgacgagaa cagagccaaa guggaaauua cgccgaauag cccccgcgcu     1140

gaggccacac uuggcggcuu cgggucucug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuagcg aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auacaggaac accccauugg     1320

aacaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gaccgucgug     1380

guucucggcu cccaggaggg ggccgugcau acggcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacacugca uggcaccgug acgguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cagcgaaucc cguuaucacc gaaucuacgg agaacuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc uggcuccacc aucgggaaag ccuuugaagc uacagugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcccucaauu cccuggggaa aggcauucac cagaucuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaacacaa agaauggguc uaucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucca cggcugugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4539
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 1 opt

<400>  4539
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccgccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gcuggggccg auacaggaac accccauugg     1320

aauaacaagg aagcgcuggu ggaguuuaaa gacgcucacg ccaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acggcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg ccgcuuucac auuuaccaaa     1560

auuccggccg aaacacugca uggcaccgug acgguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcg guggauaugc agacacugac cccugugggc     1680

cgccucauca cagccaaccc cguuaucacc gaauccacgg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcucucaacu cucuggggaa aggcauucac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaacc cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cucugggagg cguucucauc     2100

uuccugagca ccgccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4540
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 1 opt

<400>  4540
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gaaaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugagguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gacagaaaug augccgggga agcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaccu cggccacaug      240

ugugaugcca caaugagcua cgagugcccc augcuggacg aaggggugga gccggaugac      300

guggauuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgaa agccgggagu acaccaagca ccugauccgc      480

guggaaaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cuggccuacu ccauucggug caucggaguu agcaaccgcg acuuugugga gggcaugucu      660

gggggaacau gggucgaugu ggugcuugag cauggcgggu gugucaccgu uauggcucag      720

gacaagccca caguggauau ugaacuggug accaccacag ucuccaauau ggccgaggug      780

agaagcuacu gcuaugaggc uucuaucucc gacauggcca gcgauucccg guguccuacg      840

caaggagaag ccuaccucga caaacagucu gacacccagu auguuugcaa gagaacacug      900

guggaucgcg gcuggggcaa cgggugugga cuguucggca aagggagccu cgugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagcccga aaaucuggag     1020

uaccggauua ugcuuucugu ccacggcucc caacacagcg ggaugaucgu uaacgacacc     1080

ggacaugaga cggaugaaaa uagagccaaa guggagauua caccuaacuc uccgcgcgcu     1140

gaagccaccc ugggcggcuu cgggagccuc ggacuggacu gugagccccg gaccggccuu     1200

gauuuuuccg accuguauua ccucacaaug aauaacaagc acuggcuggu ccauaaagaa     1260

ugguuccacg auaucccucu gcccuggcac gccggggcug acaccggaac accucauugg     1320

aauaacaagg aggcccucgu ggaguuuaaa gaugcgcacg cuaagagaca gacggucgug     1380

guucugggcu cucaggaagg ggccgugcau accgcccuug cuggcgcccu ggaggcugaa     1440

auggacggag ccaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggau     1500

aaacugagac ucaagggagu cagcuauucu cuguguacag cggccuucac cuuuacaaaa     1560

auucccgcug agacccugca uggcacggug accguugaag ugcaguacgc cgggacagac     1620

ggaccgugca aggucccugc ucaaauggcc guggacaugc agacccucac acccgugggc     1680

cgccugauca ccgcgaaccc uguuaucacg gaguccacag agaauagcaa gaugaugcuu     1740

gaacuggauc cucccuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccucggcgau accgccuggg acuucggguc cgugggaggc     1920

gcccugaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccuu     1980

uucggcggga uguccugguu uucucaaauc cucauuggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag caucucccuu augugucugg cccucggagg cguucugauu     2100

uuccugagca ccgcuguguc ugccgauguc gggugcuccg uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4541
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 1 opt

<400>  4541
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaauu agcaaccgcg auuuuguuga aggcaugucu      660

gggggaacau ggguggacgu cgugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggauucug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuagcg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cccaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu guccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauua ccgcgaaccc cgugaucacg gaaucuacag aaaauuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auuguuaucg gcgucggaga gaaaaagauu     1800

acccaccacu ggcaucgguc uggcuccaca aucgggaaag ccuuugaggc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucgggag cgucggaggc     1920

gcccucaacu cccuggggaa aggcauccac cagauuuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacggguc uaucagccug augugucuug cccugggagg cgugcucauu     2100

uuccugucca ccgcuguuag cgccgacgug gggugcucug ucgauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4542
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 1 opt

<400>  4542
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaca      240

ugugacgcca ccaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacacuuc ccagccauuc cauccgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggauuuuuag aaauccuggc uucgcgcugg ccgcugccgc uaucgccugg      540

cuccuggggu ccagcacguc ucagaaaguc auuuaucuug ugaugauccu gcucauugcg      600

cccgccuacu ccauccggug cauuggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau cgagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucgucauug gcgugggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaaugcca agaacgggag cauuucccug augugucuug cucugggagg cguucucauc     2100

uuccugagca cggccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4543
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 1 opt

<400>  4543
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucaacgguc ucgcagagcc guuacccuuc ccagccauuc cacacggaag      420

cugcagaccc gcucucagac auggcucgag agcagagaau acaccaagca ccugauccgg      480

guggagaacu ggaucuuucg caauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauuagaug caucggaguu agcaaccggg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucaa      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

cgcagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacuccag augucccacg      840

cagggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gcggacacuc      900

guggacagag gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccaaccuga gaaucucgaa     1020

uaccgcauua ugcugucugu ccacggcucc cagcacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa ucgggccaaa guggaaauua caccgaacuc ucccagagcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg caccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagcggca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggcag acugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuucggc ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaauacgc cgggacagac     1620

ggaccuugca agguccccgc ucagauggcc guggauaugc agacccugac accugugggc     1680

agacucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgcuc cggcagcaca aucgggaaag ccuuugaagc uaccgugcgg     1860

ggagccaaga gaauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac caaaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucagauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4544
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 1 opt

<400>  4544
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaagcga gaucucagac cuggcucgag agccgggaau acacaaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcccugg cugccgcugc cauugcgugg      540

cuccuggggu ccagcaccuc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcuuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacgu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug acaaccaccg ucuccaauau ggcugaagug      780

agaagcuacu gcuaugaggc cucuaucucc gauauggcca gcgacucccg gugucccaca      840

caaggagagg cuuaccugga caaacagucu gauacgcagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcgaaguuug ccugcuccaa gaaaaugacc ggaaagagca uccagccuga aaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauaca     1080

ggacaugaga ccgacgagaa uagagcuaaa guggaaauua cgccgaacuc uccccgcgcc     1140

gaggccacac uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gcuggggccg auacaggaac accccauugg     1320

aauaacaagg aagcgcuggu ggaguuuaaa gacgcucacg ccaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acggcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg ccgcuuucac auuuaccaaa     1560

auuccggccg aaacacugca uggcaccgug acgguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcg guggauaugc agacacugac cccugugggc     1680

cgccucauca cagccaaccc cguuaucacc gaauccacgg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcucucaacu cucuggggaa aggcauucac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaauacaa agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca cggccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4545
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 1 opt

<400>  4545
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaucug acgccgggga ggcuauuagc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcag cagacgggcc guuacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggaau acaccaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggga gcucuacguc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucagcaacau ggccgaagug      780

agaucuuacu gcuaugaggc uuccaucagc gauauggccu ccgacucucg gugucccacg      840

caaggagaag ccuaccugga caaacagagc gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu ccacggcagc caacacucug ggaugaucgu uaaugauacc     1080

ggacaugaga cggacgagaa cagagccaaa guggaaauua caccgaauag cccccgcgcu     1140

gaggccaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aacaauaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggca gccaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaaagcacag agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucggag cggcuccaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa aggcauucac cagaucuucg gagcugcguu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaacacga agaauggguc caucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucua ccgcuguguc cgccgacguc gggugcagcg uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4546
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 1 opt

<400>  4546
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgc ggaaguuaca      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac aaccucuacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu guuacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacauc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggucacaugc      960

gccaaguuug cuugcagcaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu uaaugauacg     1080

ggacaugaga cagacgagaa cagagcgaaa guggaaauua ccccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac acaggggccg auacgggaac accccauugg     1320

aacaauaagg aagcccuggu ggaguuuaaa gacgcucacg ccaagagaca gaccgucgug     1380

guucucggca gccaggaggg ggcggugcau accgcucugg ccggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguacag cugccuucac cuuuacgaaa     1560

auuccggcgg aaacccugca uggcacagug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac gccugugggc     1680

cgccucauca cagcuaaccc cguuaucacc gaagggacag agaacagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg cgacucuuac auugucaucg gagugggcga gaaaaagauu     1800

acccaccacu ggcaucgguc cgggagcacc aucggaaaag ccuuugaagc gacagugaga     1860

ggcgccaagc gcauggcugu ccugggggac acggccuggg auuucggauc cgugggcggg     1920

guucucaauu cucugggcaa aggcauucac cagaucuucg gagcugccuu uaagagccug     1980

uucgggggaa uguccugguu uucucaaauc cuuauuggca cccuccugau guggcugggg     2040

cucaacacaa agaauggaag caucucccug augugucuug cccugggcgg ggugcucauu     2100

uuccugagca ccgcugucuc ugcggacgug ggcugcuccg uugauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4547
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 1 opt

<400>  4547
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgc uauggcccag      720

gauaagccua caguugacau ugagcuugug accaccacag uguccaauau ggcugaaguc      780

agaagcuacu gcuaugaggc cucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagagg cuuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcgaaguuug ccugcuccaa gaaaaugaca ggaaagagca uccagccuga aaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugcuugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagcuaaa guugaaauca caccgaacuc uccccgcgcc     1140

gaggccaccc ugggcggcuu cgggagccuc ggacuggauu gugaaccucg gaccggccuu     1200

gacuuuuccg aucuguauua ccucacaaug aauaacaagc acuggcuggc ucauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcgg cuaccggaac accccauugg     1320

aauaacaagg aagcccucgu ggaguuuaaa gaugcccacg cuaagagaca gacggucgug     1380

guccugggcu cucaggaggg ggccgugcau accgcucuug ccggcgcgcu ggaagccgag     1440

auggacggag cuaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggau     1500

aaacugagac ucaagggagu uagcuauucu cuguguacag ccgcuuucac cuuuacaaaa     1560

aucccggccg aaaccgugga cggcacgguc accguggagg ggcaguacgg aggcacagac     1620

gggccuugca agguucccgc gcaaauggcc guggauaugc agacccugac accugucggc     1680

cgccucauua ccgcuaaccc cgugaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg agacucuuac aucgugauug gcguugggga gaaaaagauc     1800

acccaucacu ggcaccgguc cggaagcaca auuggcaaag ccuuugaagc uaccgucaga     1860

ggggccaagc gcauggccgu gcugggagac accgcuuggg auuucggcuc cgucgggggc     1920

gcgcucaacu cucugggaaa aggcauccau cagauuaucg gggccgcuuu caagagccug     1980

uuuggaggca uguccugguu cucucaaauc cuuauuggga cacuccugau guggcuggga     2040

cucaauacga agaacggcag caucucccug augugucuug cccucggggg cgugcugauu     2100

uuucugagca ccgccguguc uggaggcguu gggugcuccg uggacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4548
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 1 opt

<400>  4548
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgc ggaaguuaca      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac aaccucuacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu guuacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacauc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggucacaugc      960

gccaaguuug cuugcagcaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu uaaugauacg     1080

ggacaugaga cagacgagaa cagagcgaaa guggaaauua ccccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auacaggaac gccccauugg     1320

aacaauaagg aagcucuggu ggaguuuaaa gacgcccacg cgaagagaca gaccgucgug     1380

guucucggca gccaggaggg ggcugugcau acagcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguaccg ccgcguucac auuuagcaaa     1560

auuccggccg aaacccugca uggcacggug accgaggaag uucaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgccaaccc cgugaucacg gagucuacag agaacuccaa gaugaugcug     1740

gaacuugauc ccccuuucgg ggacagcuac auuguuaucg gcgucggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcucuaca aucgggaaag cguuugaagc caccgugaga     1860

ggagcuaagc gcauggccgu gcugggcgac accgcuuggg auuucgggag cgucggaggc     1920

gcccucaauu cccuggggaa aggcauucac cagaucuucg gagccgcuuu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaacacga agaaugggag caucucucug augugucuug cgcugggagg cgugcucauu     2100

uuccugucca ccgccguuag cgcugacgug gggugcucug ucgauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4549
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 1 opt

<400>  4549
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgc ggaaguuaca      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac aaccucuacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu guuacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacauc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggucacaugc      960

gccaaguuug cuugcagcaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu uaaugauacg     1080

ggacaugaga cagacgagaa cagagcgaaa guggaaauua ccccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auacaggaac gccccauugg     1320

aacaauaagg aagcucuggu ggaguuuaaa gacgcccacg cgaagagaca gaccgucgug     1380

guucucggca gccaggaggg ggcugugcau acagcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguaccg ccgcguucac auuuaccaaa     1560

auuccggccg aaacgcugca uggcaccgug acaguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcu guggauaugc agacacugac cccugugggc     1680

cgccucauca cggccaaccc cguuaucaca gaaagcaccg agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucggag cggcuccacc aucgggaaag cguuugaagc caccgugaga     1860

ggagcuaagc gcauggccgu ccugggcgac acagcuuggg auuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa aggcauucac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaacacca agaauggguc caucagccug augugucuug cgcugggagg cguucucauu     2100

uuccugucua cagccguguc cgcugacguc gggugcagcg uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4550
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 1 opt

<400>  4550
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgu ugaagugaca      120

cggcgcggca augcguacua uauguaccuu gauagauccg acgcugggga ggccauuucu      180

uuccccacca caaugggaau gaacaaaugc uauauccaga uuauggaucu gggccacaug      240

ugugacgcca ccaugagcua cgaaugcccu augcucgaug agggggugga accggacgau      300

gucgacuguu ggugcaauac aaccuccacg ugggugguuu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc gugacacugc ccagccauuc cacccgcaaa      420

cuucaaacaa gaucucagac cuggcuggag agccgggaau acacgaagca ccucauccgc      480

gucgagaacu ggaucuuuag aaauccuggc uucgcucugg ccgcggccgc uauugccugg      540

cugcucgggu ccagcacauc ucagaaagug aucuaucugg uuaugauucu ucugaucgcu      600

cccgccuacu ccauucggug caucggagug agcaaccgcg auuuugucga aggcaugucu      660

gggggaaccu ggguggacgu gguccucgag cauggcgggu guguuacagu gauggcgcag      720

gauaagccua ccguggacau ugagcugguc accacaacgg uguccaauau ggccgaaguu      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacc      840

caaggagagg cuuaccuuga caaacagucu gauacacagu augugugcaa gagaacccug      900

gucgaccgcg gcuggggcaa cgggugugga cucuucggca aagggagccu ggugacaugc      960

gccaaguuug ccugcuccaa gaaaaugacc ggaaagagca uccagccuga aaaucuggaa     1020

uaccggauua ugcucucugu ucacggcucc caacacagcg ggaugaucgu gaacgauacg     1080

ggacaugaga cagacgagaa uagagcuaaa gucgaaauua ccccgaacuc uccccgcgcg     1140

gaggccaccc ugggcggcuu cgggagccuu ggacuggauu gugaaccucg gacaggccuc     1200

gacuuuuccg aucuguauua ccuuaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acaucccccu cccuuggcac gcuggggccg auacaggaac gccccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcucacg ccaagagaca gaccgugguu     1380

gugcugggcu cucaggaggg ggcgguccau acagcucucg ccggcgcccu ggaagcugag     1440

auggauggag ccaagggccg ccuuagcucc gggcaccuga aaugccggcu caagauggac     1500

aaacugagac uuaagggagu gagcuauucu cuguguaccg cugccuucac auuuaccaaa     1560

auuccggcgg aaacgcucca uggcaccguu acaguggagg uccaguacgc cgggaccgac     1620

ggaccuugca aggugcccgc ucaaauggcc guggauaugc agacacugac cccuguuggc     1680

cgccugauca cggcuaaccc cgucaucaca gaauccaccg agaauagcaa gaugaugcuc     1740

gagcuggauc ccccuuucgg ggacucuuac auugugaucg gcgugggaga aaagaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaaag ccuuugaggc gaccgucaga     1860

ggagccaagc gcauggcugu gcuuggcgac acagccuggg auuucggguc cguuggaggc     1920

gcucugaacu cucucgggaa aggcauucac cagaucuucg gagccgccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cgcuucucgu guggcugggc     2040

cugaauacca agaacgggag caucucccuc augugucugg cucuuggagg cguccugauu     2100

uuccucagca cagcgguguc ugccgacguu gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4551
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 1 opt

<400>  4551
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgc ggaaguuaca      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac aaccucuacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu guuacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacauc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggucacaugc      960

gccaaguuug cuugcagcaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu uaaugauacg     1080

ggacaugaga cagacgagaa cagagcgaaa guggaaauua ccccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auacaggaac gccccauugg     1320

aacaauaagg aagcucuggu ggaguuuaaa gacgcccacg cgaagagaca gaccgucgug     1380

guucucggca gccaggaggg ggcugugcau acagcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguaccg ccgcguucac auuuaccaaa     1560

auuccggccg aaacgcugca uggcaccgug acaguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcu guggauaugc agacacugac cccugugggc     1680

cgccucauca cggccaaccc cguuaucaca gaaagcaccg agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucggag cggcuccacc aucgggaaag cguuugaagc caccgugaga     1860

ggagcuaagc gcauggccgu ccugggcgac acagcuuggg auuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa aggcauucac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaacacca agaauggguc caucagccug guuugucuug cgcugggagg cgugcucauu     2100

uuccugucua cagccgucuc cgcugacgug gggugcagcg uugauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4552
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 1 opt

<400>  4552
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgu ugaagugaca      120

cggcgcggcu ccgcguacua uauguaccuu gauagaucug acgcugggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

gucgacuguu ggugcaacac aaccucuacg ugggugguuu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcag cagacgggcc gugacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

gucgagaauu ggaucuuuag aaacccuggc uucgcucugg ccgcggccgc uauugccugg      540

cuccugggga gcucuacauc ccagaaagug aucuaucuug uuaugauucu gcucaucgcu      600

cccgccuaca gcauucggug caucggagug ucuaaucgcg auuuugucga aggcaugucc      660

gggggaaccu ggguggacgu gguccuggag cauggcgggu guguuacagu gauggcgcag      720

gauaagccug ccguggacau ugagcuuguc accaccacag ugagcaacau ggcugaaguu      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacg      840

caaggagaag ccuaccugga caaacagagc gauacccagu augugugcaa gagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggugaccugc      960

gccaaguuug cuugcagcaa gaaaaugaca ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu ucacggcagc caacacucug ggaugaucgu gaaugauacc     1080

ggacaugaga cggacgagaa cagagcgaaa gucgaaauua caccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugacaaug aacaauaagc acuggcucgu gcauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auaccggaac accccauugg     1320

aacaauaagg aagcucuggu cgaguuuaaa gacgcccacg cgaagagaca gacggugguu     1380

gugcucggca gccaggaggg ggcuguccau accgcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gucuuauucc cucuguacag ccgcguucac cuuuacaaaa     1560

auuccggccg aaacccugca uggcacgguu accguggagg uccaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu guggauaugc agacccugac accuguuggc     1680

cgccucauca ccgccaaccc cgucaucacg gaaagcacag agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugugaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucggag cggcuccaca aucgggaaag cguuugaagc caccgucaga     1860

ggagcuaagc gcauggccgu gcugggcgac accgcuuggg auuucggguc uguuggaggc     1920

gcccucaaua gccuggggaa aggcauucac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cacuccuggu guggcugggc     2040

cucaacacga agaauggguc caucagccug accugucuug cgcugggagg cguccucauu     2100

uuccugucua cagccguguc cgcugacguu gggugcagcg uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4553
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 1 opt

<400>  4553
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggu ugaagugacc      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua caauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

gucgacuguu ggugcaacac cacaucuacc ugggugguuu auggaacgug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu gugacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggaau acaccaagca ccugauccgc      480

gucgagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugu gauugccugg      540

cuccugggga gcucuacguc ccagaaaguu aucuaucuug ugaugauucu gcucaucgcu      600

cccgccuaca gcauucggug caucggaguc ucuaaucgcg auuuugugga aggcaugucc      660

gggggaacau ggguggacgu cguucuggag cauggcgggu gugugaccgu gauggcgcag      720

gauaagccua cagucgacau ugagcuugug accaccacag uuagcaacau ggccgaagug      780

agaucuuacu gcuaugaggc uuccaucagc gauauggccu ccgacucucg gugucccacg      840

caaggagagg cuuaccugga caaacagagc gauacccagu augucugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu gguuaccugc      960

gccaaguuug ccugcagcaa gaaaaugaca ggaaagucua uccagccuga aaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu caaugauacc     1080

ggacaugaga cggacgagaa cagagcuaaa guggaaauua caccgaauag cccccgcgcg     1140

gaggccaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gcuggggccg auaccggaac accccauugg     1320

aacaauaagg aagcccuggu ggaguuuaaa gacgcucacg ccaagagaca gacgguugug     1380

guccucggca gccaggaggg ggcggugcau accgcucugg ccggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu uucuuauucc cucuguacag cugccuucac cuuuacaaaa     1560

auuccggcgg aaacccugca uggcacggug accgucgagg ugcaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc guugauaugc agacccugac accugucggc     1680

cgccucauca ccgcuaaccc cgugaucacg gaaagcacag agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugugaucg gcgucggaga gaaaaagauu     1800

acccaccacu ggcaucggag cggcuccaca aucgggaaag ccuuugaagc gaccgugaga     1860

ggagccaagc gcauggcugu ucugggcgac accgccuggg auuucggguc ugugggaggc     1920

gcucucaaua gccuggggaa aggcauucac cagaucuucg gagccgccuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cacuccuggu cuggcugggc     2040

cucaacacga agaauggguc caucagccug accugucuug cucugggagg cgugcucauu     2100

uuccugucua cagcgguuuc cgccgacgug gggugcagcg ucgauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4554
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 1 opt

<400>  4554
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggu ugaagugacc      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacaa cccugggagu gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua caauguccua cgaaugcccu augcuggaug agggggucga accggacgau      300

guggacuguu ggugcaacac cacaucuacc uggguugugu auggaacgug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu gucacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggaau acaccaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacguc ccagaaaguu aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguc ucuaaucgcg auuuugugga aggcaugucc      660

gggggaacau ggguggacgu cguucuggag cauggcgggu gugugaccgu gauggcccag      720

gauaagccua cagucgacau ugagcuugug accaccacag uuagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacg      840

caaggagaag ccuaccugga caaacagagc gauacccagu augucugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu gguuaccugc      960

gccaaguuug cuugcagcaa gaaaaugaca ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu caaugauacc     1080

ggacaugaga cggacgagaa cagagcgaaa guggaaauua caccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auaccggaac accccauugg     1320

aacaauaagg aagcucuggu ggaguuuaaa gacgcccacg cgaagagaca gacgguugug     1380

guccucggca gccaggaggg ggcugugcau accgcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu uucuuauucc cucuguacag ccgcguucac cuuuacaaaa     1560

auuccggccg aaacccugca uggcacggug accgucgagg ugcaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu guugauaugc agacccugac accugucggc     1680

cgccucauca ccgccaaccc cgugaucacg gaaagcacag agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugugaucg gcgucggaga caaaaagauu     1800

acccaccacu gguaucggag cggcuccaca aucgggaaag cguuugaagc caccgugaga     1860

ggagcuaagc gcauggccgu ucugggcgau accgcuuggg acuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa aggcauucau cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cacuccuggu cuggcugggc     2040

cucaacacga agaauggguc caucagccug accugucuug cgcugggagg cgugcucauu     2100

uuccugucua cagccguuuc cgcugaugug gggugcagcg ucgacuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4555
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 1 opt

<400>  4555
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggu ugaagugacc      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggaa agccauuagc      180

uuccccacaa cccugggagu gaauaagugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua caauguccua cgagugcccu augcuggaug aaggggucga gccggacgau      300

guggacuguu ggugcaacac cacaucuacc uggguugugu auggaacgug ucaucacaaa      360

aagggcgaag cccggcgcag cagacgggcu gucacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggagu acaccaaaca ccugauccgc      480

guggaaaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacguc ccagaagguu aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaacau ggguggacgu cguucuggaa cauggcgggu gugugaccgu gauggcccag      720

gauaaaccua cagucgacau ugagcuugug accaccacag uuagcaacau ggcugaggug      780

agaucuuacu gcuaugaagc cuccaucagc gauauggcuu ccgacucucg gugucccacg      840

caaggagagg ccuaccugga caagcagagc gauacccagu augucugcaa aagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuaccugc      960

gccaaguuug cuugcagcaa aaagaugaca ggaaaaucua uccagccuga aaaccucgag     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu caaugauauu     1080

ggacaugaaa ccgacgagaa cagagcgaag guggagauca cgccgaauag cccccgcgcc     1140

gaagcuacac uuggcggcuu cgggucccug ggacucgauu gugagccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaaac acuggcucgu ccauaaggaa     1260

ugguuccacg acauuccccu gccuuggcac gccggggccg auacaggaac accccauugg     1320

aacaauaaag aggcucuggu ggaauuuaag gacgcccacg cgaagagaca gacaguugug     1380

guccucggca gccaggaggg ggcugugcau acggcccugg ccggcgcucu ugaggccgaa     1440

auggauggag cuaaaggccg ccuguccagc gggcaccuca agugccggcu gaaaauggac     1500

aaacuuagac ugaagggagu uucuuauucc cucuguaccg ccgcguucac auuuaccaag     1560

aucccggccg agacacugca uggcaccgug acggucgaag ugcaguacgc ugggaccgac     1620

ggaccuugca aggugcccgc ccaaauggcu guugauaugc agacacugac cccugucggc     1680

cgccucauca cagccaaccc cgugauuacc gagagcacgg aaaacucuaa aaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac aucgugauug gcgucggaga caagaaaauc     1800

acacaccacu ggcaucggag cggcuccacc auugggaagg cguuugaggc cacagugaga     1860

ggagcuaaac gcauggccgu ucugggcgau accgcuuggg acuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa gggcauccac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauu cuuaucggaa cccuccuggu cuggcugggc     2040

cucaacacca aaaauggguc cauuagccug acgugucuug cgcugggagg cgugcucauc     2100

uuccugucua ccgccguuuc cgcugaugug gggugcagcg ucgacuuuuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4556
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 1 opt

<400>  4556
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucacgcugg ucgccguggc uaucgccugg      540

cuccugggga gcucuacauc ccagaagguu auuuaccuug ugaugauccu gcucauugcu      600

ccugccuaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaaccu ggguggacgu cguucuggaa cacggcgggu gugugacagu gauggcgcag      720

gauaaaccca ccgucgacau cgagcuugug accacaacgg uuagcaacau ggccgaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacc      840

caaggagagg cuuaucugga caagcagagc gauacacagu acgucugcaa aagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuacaugc      960

gccaaguuua ccugcagcaa aaagaugacg ggaaaaucua uucagcccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc caacauucug ggaugauugu caaugauaca     1080

ggacacgaaa ccgacgagaa cagagccaag guggagguca ccccuaauag cccgcgcgcu     1140

gaagcgacac uuggcggcuu cgggucccug ggacucgauu gugagccccg gaccggccug     1200

gacuuuucug aucuuuacua ucugacaaug aacaauaaac auuggcucgu gcacaaggaa     1260

ugguuccacg acaucccucu gcccuggcau gccggggcug auacgggaac cccucacugg     1320

aacaauaaag aggcccuggu ugaauuuaag gacgcccaug cuaagagaca gacagugguc     1380

gugcucggca gccaggaggg ggccguucac accgcgcugg cuggcgcccu ugaggccgaa     1440

auggauggag cuaaaggccg ccuguucucc gggcaucuca agugccggcu gaaaauggac     1500

aaacuuagac ugaaaggagu gagcuacucu cucuguacag ccgcuuuuac cuucacgaag     1560

guccccgccg agacccugca cggcacagug accguggaag uucaguaugc ggggacagac     1620

ggaccgugca aggucccugc ccaaauggcu guggauaugc agacccugac gcccgugggc     1680

cgccucauua cagccaaccc ugucaucacc gaguccacag aaaacagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgugauug gcguuggaga caagaaaauc     1800

acccaccauu ggcaccgguc cggcagcacc auugggaagg cuuucgaggc cacagugaga     1860

ggagcgaaac gcauggccgu ccugggcgau acggcuuggg acuucggguc cgugggaggc     1920

guuuuuaauu cucucgggaa gggcauccau cagauuuucg gagccgcuuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cugaucggaa cccuucucgu guggcugggc     2040

cugaacacaa agaaugggag cauuucccuc accugucugg cccuuggagg cgucaugauc     2100

uuccugagca cagccguguc ugcugauguu gggugcuccg uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4557
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 1 opt

<400>  4557
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa aucugggagu gaacaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua ccauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaauac aaccucuaca uggguggucu acggaaccug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacgcuuc cgucccacuc uaccagaaag      420

cugcaaacac ggagccagac cuggcucgag ucccgcgagu auacaaagca ucugauuaaa      480

guugaaaacu ggaucuuuag aaaucccggc uucgcccugg cugccguggc uauugccugg      540

cuccugggga gcucuaccuc ccagaagguc aucuaccuug ugaugauucu gcucaucgcg      600

ccugccuaua gcauucggug caucggaguu ucuaaccgcg auuuugugga gggcaugucc      660

gggggaacgu gggucgacgu ggugcuggaa cacggcgggu gugucacagu uauggcucag      720

gauaaaccca ccguggacau cgagcuugug acaaccaccg ucagcaauau ggccgaggug      780

agaucuuacu gcuacgaggc uuccauuagc gauauggccu ccgacucucg guguccuaca      840

caaggagaag ccuaucugga caagcagagc gauacgcagu acguuugcaa aagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aggggucccu ggucacaugc      960

gcuaaguuua ccugcagcaa aaagaugaca ggaaaaucua uccagcccga aaaucucgag     1020

uaucggauua ugcuguccgu gcacggcagc caacauucug ggaugaucgu uaacgauauu     1080

ggacacgaaa ccgacgagaa uagagcgaag guggagguca cgccuaacag cccgcgcgcc     1140

gaagcuacac uuggcggcuu cgggucccug ggacucgauu gugagccccg gaccggccug     1200

gacuuuucug aucuuuacua ucugaccaug aauaacaaac auuggcucgu gcacaaggaa     1260

ugguuccacg acaucccucu gcccuggcau gccggggccg auacaggaac cccucacugg     1320

aauaacaaag aggcucuggu cgaauuuaag gacgcccaug cgaagagaca gacagugguu     1380

gugcucggca gccaggaggg ggcuguccac acggcccugg ccggcgcucu ugaggccgaa     1440

auggauggag cuaaaggccg ccuguucucc gggcaucuca agugccggcu gaaaauggac     1500

aaacuuagac ugaaaggagu gagcuacucu cucuguaccg ccgcguuuac auucaccaag     1560

guucccgccg agacacugca cggcaccgug acggucgaag ugcaguaugc ugggaccgac     1620

ggaccgugca aggugccugc ccaaauggcu guugauaugc agacacugac ccccgucggc     1680

cgccucauca cagccaaccc ugugauuacc gaguccacgg aaaauagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgugauug gcgucggaga caagaaaauc     1800

acacaccauu ggcaccgguc cggcagcacc auugggaagg cguucgaggc cacagugaga     1860

ggagcuaaac gcauggccgu ucugggcgau accgcuuggg acuucggguc cgugggaggc     1920

gucuuuaacu cucucgggaa gggcgugcau cagaucuucg gagccgccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cccuucucgu uuggcugggc     2040

cugaauacaa agaacgggag caucucccuc acgugucugg cucuuggagg cgugaugauu     2100

uuccugagca ccgcggucuc ugccgaugug gggugcuccg uugacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4558
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 1 opt

<400>  4558
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgu gaaugauauu     1080

ggacacgaaa cggacgagaa cagagcuaag gucgagguga caccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gaccggccug     1200

gacuuuucug aucuuuacua ucugacaaug aacaauaaac auuggcucgu ccacaaggaa     1260

ugguuccacg acaucccucu gcccuggcau gcuggggccg auaccggaac accucacugg     1320

aacaauaaag aggcccuggu ggaauuuaag gacgcucaug ccaagagaca gacgguugug     1380

guccucggca gccaggaggg ggcggugcac accgcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggccg ccuguucucc gggcaucuca agugccggcu gaaaauggac     1500

aaacuuagac ugaagggagu uagcuacucu cucuguacag cugccuuuac cuucacaaag     1560

gugcccgcgg agacccugca cggcacgguc accguggaag ugcaguaugc cgggacagac     1620

ggaccgugca agguuccugc ucaaauggcc gucgauaugc agacccugac acccgugggc     1680

cgccucauca ccgcuaaccc ugugauuacg gaguccacag aaaacagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgucauug gcgugggaga caagaaaauc     1800

acccaccauu ggcaccgguc cggcagcaca auugggaagg ccuucgaggc gaccguuaga     1860

ggagccaaac gcauggcugu gcugggcgau accgccuggg acuucggguc cgucggaggc     1920

guguuuaauu cucucgggaa gggcauccau cagaucuucg gagcugccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cacuucucgu uuggcugggc     2040

cugaacacga aaaaugggag cauuucccuc accugucugg cccuuggagg cgugaugauc     2100

uuccugagca cagcugucuc ugcggaugug gggugcuccg uugacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4559
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 1 opt

<400>  4559
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa aucugggagu gaacaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua ccauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaauac aaccucuaca uggguggucu acggaaccug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacgcuuc cgucccacuc uaccagaaag      420

cugcaaacac ggagccagac cuggcucgag ucccgcgagu auacaaagca ucugauuaaa      480

guugaaaacu ggaucuuuag aaaucccggc uucgcccugg cugccguggc uauugccugg      540

cuccugggga gcucuaccuc ccagaagguc aucuaccuug ugaugauucu gcucaucgcg      600

ccugccuaua gcauucggug caucggaguu ucuaaccgcg auuuugugga gggcaugucc      660

gggggaacgu gggucgacgu ggugcuggaa cacggcgggu gugucacagu uauggcucag      720

gauaaaccca ccguggacau cgagcuugug acaaccaccg ucagcaauau ggccgaggug      780

agaucuuacu gcuacgaggc uuccauuagc gauauggccu ccgacucucg guguccuaca      840

caaggagaag ccuaucugga caagcagagc gauacgcagu acguuugcaa aagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aggggucccu ggucacaugc      960

gcuaaguuua ccugcagcaa aaagaugaca ggaaaaucua uccagcccga aaaucucgag     1020

uaucggauua ugcuguccgu gcacggcagc caacauucug ggaugaucgu uaacgaugaa     1080

aauagagcga agguggaggu caccccuaac agcccgcgcg ccgaggcuac gcuuggaggc     1140

uucggcuccc uggggcucga cugugaaccc cggacaggac uggauuuuuc ugaccuuuac     1200

uaucugacca ugaauaacaa acacuggcuc gugcauaagg agugguucca cgauauuccu     1260

cugcccuggc acgccggcgc cgacaccggg acaccucauu ggaauaacaa agaagcucug     1320

gucgaguuua aggaugccca cgcgaagaga cagaccgugg uugugcucgg aagccaggaa     1380

ggcgcugucc auacagcccu ggccggggcu cuugaggccg agauggacgg cgcuaaagga     1440

cgccuguucu ccggccaccu caagugccgg cugaaaaugg auaaacuuag acugaagggg     1500

gugagcuacu cucucuguac ggccgcguuu accuucacaa agguucccgc cgaaacccug     1560

cauggaacag ugaccgucga ggugcaguau gcuggcacgg acgggccgug caaggugccu     1620

gcccaaaugg cuguugacau gcagacccug acacccgucg gacgccucau caccgccaac     1680

ccugugauca cagaauccac cgagaauagc aaaaugaugc uggaacuuga uccucccuuu     1740

ggcgacucuu acauugugau cggggucggc gauaagaaaa uuacgcacca cuggcaucgg     1800

uccggaagca caaucggcaa ggcguucgag gccaccguga gaggggcuaa acgcauggcc     1860

guucugggag acacagcuug ggauuucggc uccgucgggg gaguguuuaa cucucucggc     1920

aaggggauuc accagaucuu cggcgccgcc uuuaagagcc uguucggagg cauguccugg     1980

uuuucucaaa uccugauugg gacccuucuc guguggcugg gacugaauac caaaaacggc     2040

agcaucuccc ucacaugucu ggcucuuggg ggaguuauga uuuuccugag cacggcggug     2100

ucugccgacg ucggcugcuc cguggauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4560
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 1 opt

<400>  4560
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucgcccugg ucgcuguggc caucgcuugg      540

cuccugggga gcucuacguc ccagaagguu auuuaccuug ugaugauccu gcucauugcc      600

ccugcguaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaacau ggguggacgu cguucuggaa cacggcgggu gugugaccgu gauggcccag      720

gauaaaccca cagucgacau cgagcuugug accaccacag uuagcaacau ggcugaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgucugcaa aagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uucagcccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc caacauucug ggaugauugu caaugauacg     1080

ggacacgaaa cagacgagaa cagagcuaag guggagguca ccccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gacaggccug     1200

gacuuuucug aucuuuacua ucugaccaug aacaauaaac auuggcucgu gcacaaggaa     1260

ugguuccacg acaucccucu gcccuggcau gcuggggccg auacaggaac gccucacugg     1320

aacaauaaag aggcccuggu ugaauuuaag gacgcucaug ccaagagaca gaccgugguc     1380

gugcucggca gccaggaggg ggcgguucac acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggccg ccuguucucc gggcaucuca agugccggcu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacucu cucuguaccg cugccuuuac auucaccaag     1560

guccccgcgg agacgcugca cggcaccgug acaguggaag uucaguaugc cgggaccgac     1620

ggaccgugca aggucccugc ucaaauggcc guggauaugc agacacugac ccccgugggc     1680

cgccucauua cggcuaaccc ugucaucaca gaguccaccg aaaacagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgugauug gcguuggaga caagaaaauc     1800

acacaccauu ggcaccgguc cggcagcacc auugggaagg ccuucgaggc gaccgugaga     1860

ggagccaaac gcauggcugu ccugggcgau acagccuggg acuucggguc cgugggaggc     1920

guuuuuaauu cucucgggaa gggcauccau cagauuuucg gagcugccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cugaucggaa cgcuucucgu guggcugggc     2040

cugaacacca aaaaugggag cauuucccuc acaugucugg cccuuggagg cgucaugauc     2100

uuccugagca ccgcuguguc ugcggauguu gggugcuccg uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4561
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 1 opt

<400>  4561
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaacacaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccauuc uacaagaaag      420

cugcagaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ccugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucgcccugg ucgcuguggc caucgcuugg      540

cuccugggga gcucuacguc ccagaagguu auuuaccuug ugaugauccu gcucauugcc      600

ccugcguaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaacau ggguggacgu cguucuggaa cauggcgggu gugugaccgu gauggcccaa      720

gauaaaccca cagucgacau cgagcuugug accaccacag uuagcaacau ggcugaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacg      840

cagggagagg ccuaucugga caagcagagc gauacccagu acgucugcaa aagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uucaacccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc cagcacucug ggaugauugu caaugauauc     1080

ggacaugaaa cggacgagaa cagagcuaag guggagguca caccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gaccggccug     1200

gacuuuucug aucuuuacua ucugacaaug aacaauaaac acuggcucgu gcauaaggaa     1260

ugguuccacg acauuccucu gcccuggcac gcuggggccg auaccggaac accucauugg     1320

aacaauaaag aggcccuggu ugaauuuaag gacgcucacg ccaagagaca gacggugguc     1380

gugcucggca gccaggaggg ggcgguucau accgcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggccg ccuguucucc gggcaccuca agugccggcu gaaaauggac     1500

aaacuuagac ugaaaggagu gagcuacucu cucuguacag cugucugcac cgccgcgaag     1560

gugcccgccg agacacugca uggcaccgug acgguugaag uccaauaugc ugggaccgac     1620

ggaccgugua aggugccugc ccagauggcu guggauaugc agacacugac ccccgucggc     1680

cgccucauca cagccaaccc ugugaucacc gaguccacgg aaaacagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac auuguuaucg gcgugggaga caagaaaauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cguucgaggc cacagucaga     1860

ggagcuaaac gcauggccgu gcugggcgau accgcuuggg acuuuggguc cguuggaggc     1920

guguucaauu cucucgggaa gggcauucac caaaucuucg gagccgccuu uaagagccug     1980

uucggcggga uguccugguu uucucagauc cugauuggaa cccuucucgu cuggcugggc     2040

cugaacacaa agaaugggag caucucccuc acgugccugg cucuuggagg cgugaugauu     2100

uuccugagca ccgcgguuuc ugccgaugug ggguguuccg ucgacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4562
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 1 opt

<400>  4562
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacgccagca ucgggauugu gucccugcuc cugaccacag ucauggcggc ugaaaucacg      120

cgcagaggau cugccuacua uauguaccuu gaucggagcg acgccggcaa ggcuauuucc      180

uucguuacca cacugggggu gaauaaaugc cacgugcaga ucauggaucu cggacauaug      240

ugugacgcca ccaugucuua ugagugcccc augcuggaug aaggcgucga gcccgacgau      300

guggacuguu ggugcaacac aaccagcaca uggguugugu acgggaccug ucaccauaag      360

aaaggagaag cucgcagauc ccggcgcgcc gucacgcuuc cgucucacag caccagaaag      420

cugcaaacac ggucccagac cuggcucgag agccgcgagu auacaaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucgcgcugg uugccguggc uaucgccugg      540

cuccugggau cuuccaccag ccagaagguc auuuaccuug ugaugauccu gcucauugcu      600

ccugccuauu cuauccggug cauuggggug uccaaucgcg auuuugucga gggaaugagc      660

ggcgggacgu ggguugacgu ggugcuggaa cacggaggcu gugucacagu gauggcgcag      720

gauaaaccca ccguugacau cgagcuugug acaaccaccg ucucuaacau ggccgaggug      780

agauccuacu gcuacgaagc uagcaucucc gauauggccu cugacagccg guguccuaca      840

caaggggagg cuuaucugga caagcagucc gauacgcagu acguuugcaa aagaacccuc      900

guggaccgcg gauggggcaa uggcuguggg cuguucggaa agggcagccu ggucacaugc      960

gccaaguuua ccugcucuaa aaagaugaca gggaaaucca uucagcccga aaaccucgag     1020

uaucggauca ugcugagcgu gcacggaucu caacauagcg gcaugauugu caaugaugaa     1080

aacagagcca agguggaggu uaccccuaau uccccgcgcg cugaggcgac gcuuggggga     1140

uucggcucuc ugggccucga cugugaaccc cggacagggc uggauuuuag cgaccuuuac     1200

uaucugacca ugaacaauaa acacuggcuc gugcauaagg agugguucca cgauaucccu     1260

cugcccuggc acuccggagc cgacaccgaa acaccucauu ggaacaauaa agaggcucug     1320

gucgaauuua aggaugccca cgccaagaga cagaccgugg uugugcucgg cagccaggag     1380

ggggcugucc auacagcccu ggcgggagcu cuugaggccg aaauggacgg cgccaaaggg     1440

cgccugucuu ccggccaccu caagugccgg cugaaaaugg auaaacuuag acugaaagga     1500

gugagcuacu cucucuguac ggcugccuuc accuuuacaa aggugcccgc ugagacccug     1560

cauggcacag uuaccgucga agugcaguau gccgggcgcg acggaccgug caaggugccu     1620

gcgcaaaugg ccgucgacau gcagacgcug acccccgugg gccggcucau uacagcuaac     1680

ccuguuauca ccgaguccac agaaaacagc aaaaugaugc uggagcuuga uccucccuuc     1740

ggggacuccu acaucgugau uggagucggc gauaagaaaa ucacccacca cuggcauaga     1800

ucugggagca uuaucggcaa ggccuuugag gcuacggugc gcggagccaa acggauggcg     1860

guucugggcg acacagccug ggauuucggg uccgugggag gcgucuucaa uucucucggg     1920

aagggcauuc accagaucuu uggcgcugcc uucaagagcc uguuuggggg cauguccugg     1980

uucagccaaa uccugauugg aacccuucuc guguggcugg gccugaacac aaagaauggg     2040

ucuaucuccc ucaccugucu ggcucuugga ggcguuauga uuuuucugag caccgccgug     2100

ucugccgacg ucgggugcuc cguggauuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4563
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 1 opt

<400>  4563
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucgcccugg ucgcuguggc caucgcuugg      540

cuccugggga gcucuacguc ccagaagguu auuuaccuug ugaugauccu gcucauugcc      600

ccugcguaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaacau ggguggacgu cguucuggaa cacggcgggu gugugaccgu gauggcccag      720

gauaaaccca cagucgacau cgagcuugug accaccacag uuagcaacau ggcugaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgucugcaa aagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uucagcccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc caacauucug ggaugauugu caaugaugaa     1080

aacagagcua agguggaggu cacgccuaau agcccgcgcg cggaggccac acuuggaggc     1140

uucggcuccc uggggcucga cugugaaccc cggaccggac uggauuuuuc ugaccuuuac     1200

uaucugacca ugaacaauaa acacuggcuc gugcauaagg agugguucca cgauaucccu     1260

cugcccuggc acgcuggcgc cgacacaggg accccucauu ggaacaauaa agaagcccug     1320

guugaguuua aggaugcuca cgccaagaga cagacagugg ucgugcucgg aagccaggaa     1380

ggcgcgguuc auacggcucu ggccggggcc cuugaggcug agauggacgg cgccaaagga     1440

cgccuguucu ccggccaccu caagugccgg cugaaaaugg auaaacuuag acugaaaggg     1500

gugagcuacu cucucuguac cgcugccuuu acauucacca agguccccgc ggaaacacug     1560

cauggaaccg ugacggugga gguucaguau gccggcaccg acgggccgug caaggucccu     1620

gcucaaaugg ccguggacau gcagacacug acccccgugg gacgccucau uacagcuaac     1680

ccugucauca ccgaauccac ggagaacagc aaaaugaugc uggaacuuga uccucccuuu     1740

ggcgacucuu acaucgugau ugggguuggc gauaagaaaa ucacacacca cuggcaucgg     1800

uccggaagca ccauuggcaa ggccuucgag gcgacaguga gaggggccaa acgcauggcu     1860

guccugggag acaccgccug ggauuucggc uccguugggg gaguguuuaa uucucucggc     1920

aaggggaucc accagauuuu cggcgcugcc uuuaagagcc uguucggagg cauguccugg     1980

uuuucucaaa uccugaucgg gacccuucuc guguggcugg gacugaacac aaagaauggc     2040

agcauuuccc ucacgugucu ggcccuuggg ggagucauga ucuuccugag caccgcugug     2100

ucugcggacg uuggcugcuc cguggauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4564
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 1 opt

<400>  4564
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gccugacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaaa cccgcagaag ccggcgcucc gugucucuua gauaucacua cacacggaag      420

cugcaaaccc gcagccagac auggcucgag uccagagagu auaagaaaca ucugauuaug      480

guugaaaauu ggaucuuucg gaacccgggc uucgccauug ugagcgucgc gaucaccugg      540

cugauggggu cucucacguc ccagaaggug auuuaccugg uuaugaucgu gcuuauuguc      600

cccgccuaca gcaucucuug caucggagug uccaaucgcg aucuggugga gggcaugagc      660

gggggaacau gggucgacgu ugugcucgaa cacggcgggu gugugaccga gauggcucag      720

gauaaaccua cagucgacau ugagcuggug accaugaccg uuucuaacau ggccgaagug      780

agauccuauu gcuacgaggc uagccuuucc gauauggccu cugcgagccg gugucccaca      840

caaggagaac cuucccugga caagcagagc gacacgcagu cugucugcaa aagaacccuc      900

ggcgaucgcg gcugggggaa uggauguggc aucuuuggga agggaucccu ggugacaugc      960

agcaaguuua ccugcuguaa aaagaugccc ggcaaaucua uucagccuga gaaccuggaa     1020

uaucggauca ugcucccggu ucacgggagc caacauuccg gaaugauugu gaaugacauc     1080

ggccacgaga cagaugagaa cagagccaag gucgaaguga cccccaauuc uccucgcgcu     1140

gaggccacgc ugggcggguu uggaagccuu ggccuggacu gcgaaccccg gacagggcuc     1200

gauuucuccg accuguacua ucuuaccaug aacaauaaac auuggcuggu ccacaaggag     1260

ugguuucacg auaucccucu ccccuggcau gcuggagccg acaccggcac accgcacugg     1320

aacaauaaag aagcccuggu ggaguucaag gaugcucaug cgaagagaca gaccguugug     1380

guccugggga gccaggaggg cgccgugcac acagcucucg ccggagcccu ggaagcugag     1440

auggacggcg ccaaagggcg ccuuuucucu ggacaucuga agugucggcu caaaauggau     1500

aagcugagac uuaaaggcgu uuccuacagc cugugcacgg cggcuuucac cuuuacaaag     1560

gugccugccg aaacccucca cgggacaguc accguggagg ugcagucugc cggaacggac     1620

ggccccugua agguuccugc ucaaauggcc gucgacaugc agacccugac acccgugggg     1680

cgccugauua ccgcuaaccc cgugaucaca gaauccaccg agaacagcaa aaugaugcuc     1740

gagcuggauc cuccguucgg cgacuccuau auugucaucg gagugggcga uaagaaaauu     1800

acgcaccauu ggcaccgguc ugggagcaca aucggaaagg ccuuugaagc gaccguuaga     1860

ggcgccaaac gcauggcugu gcuuggggac acagccuggg auuucggauc cgucggcggg     1920

guguucaauu cucugggcaa gggaauccau cagauuuuug gcgcugccuu caagagccuc     1980

uuugggggaa uguccugguu cagccaaauc cugauuggca cccugcuugu uuggcucggg     2040

cugaacacca aaaauggauc uaucucccug acaugccucg cgcugggcgg ggugaugauu     2100

uuucuuagca cggccgucuc ugcugacgug ggcuguuccg uugauuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4565
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 1 opt

<400>  4565
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa ccaugggagu gaauaaaugc cacguccaga ucauggaucu gggccauaug      240

ugugacgcua caauguccua ugagugcccc augcucgaug aagggguuga gccugacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcccgag cagacgggcg gugacccugc ccucccacuc uacacgcaaa      420

cuucaaacca gaagccagac auggcuggag ucccgggagu auaccaagca ucucauuaag      480

guugaaaauu ggauccugcg caacccuggc uuuacggugg ccgcucuggc cauggcuugg      540

cuccugggga auagcacauc ucagagaguc auuuaccuug ugaugauccu gcucauugcc      600

cccgcguauu ccauccggug cauuggaguu agcaaccgcg auuucgugga gggcaugucu      660

gggggaaccu gggucgacgu ggugcuggaa cacggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau cgagcuugug accacaacgg ucuccaauau ggcugaggug      780

agaagcuacu gcuacgaagc cucuaucucc gauauggcua gcgacucccg gugucccacc      840

caaggagagg ccuaucugga caaacagucu gauacacagu acguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguuuggca aagggagccu ggucacaugc      960

gccaaguuua ccugcuccaa gaaaaugacg ggaaagagca uucagccuga aaaucucgag     1020

uaucggauca ugcugucugu gcacggcucc caacauagcg ggaugauugu uaacgauauc     1080

ggacacgaaa cagacgagaa uagagcuaaa guggagguca ccccgaacuc uccccgcgcg     1140

gaagccaccc uuggcggcuu cgggagccug ggacucgauu gugagccucg gacaggccug     1200

gacuucuccg aucuuuacua ucugaccaug aauaacaagc auuggcucgu gcacaaagaa     1260

ugguuucacg acauuccccu gccuuggcau gcuggggccg auacaggaac gccccacugg     1320

aauaacaagg aggcccuggu cgaguucaaa gacgcucaug ccaagagaca gaccgugguu     1380

gugcucggcu cucaggaagg ggcgguccac acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaagggccg ccuguuuagc gggcaucuca aaugccggau caagauggac     1500

aaacugagac uuaagggagu guccuacagc cuguguaccg cugccuucac auuuucuaaa     1560

guuccggcgg agacccucca cggcacggug accgucgaag ugcaguaugc cgggcgcgac     1620

ggaccuugca aggugcccgc ucaaauggcc guugauaugc agacacugac cccugucggc     1680

cggcugauca cagcuaaccc cauuaucacc gaguccacgg aaaauagcag aaugaugcuc     1740

gagcuggauc ccccuuucgg ggacucuuac auugugaucg gcgugggaga caagaaaauu     1800

acacaccauu ggcaccgcuc cggcagcacc aucgggaagg ccuuugaggc gacaguccgg     1860

ggagccaaaa gaauggcugu gcuuggcgau accgccuggg acuucggguc cguuggaggc     1920

guguucaacu cucuggggaa gggcguccau cagguguuug gagcugccuu caaaagccuc     1980

uuuggcggga uguccugguu cucucaaauc cugauuggaa cccugcuugu uuggcucggc     2040

cugaauacaa agaacgggag caucucccug gccugucucg cucugggagg cgcgaugauu     2100

uuucuuagca cggccguguc ugcugauguc gggugcuccg uggacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  4566
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 1 opt

<400>  4566
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4567
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 1 opt

<400>  4567
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccguug ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa cgcuaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu gagcaccgcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4568
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 1 opt

<400>  4568
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucggguacg agacggacga agauagagcc aagguggagg ucacaccuaa cagcccgcgg     1080

gccgaggcua cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga     1140

cuggauuuuu cugaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag     1200

gagugguucc acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau     1260

uggaauaaca aagaagcucu ggucgaguuu aaggaugccc acgccaaacg ccagacggug     1320

guugugcucg gaagccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggacg gcgccaaggg acggcuguuc uccggccacc ucaagugcag acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucucucugua cagcugccuu uaccuucaca     1500

aagguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gacgggccgu gcaaaauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacggcuca uuaccgccaa uccugugauc acggaaucca cagagaacag caagaugaug     1680

cuggaacuug auccucccuu uggcgacucu uacaucguga uuggggucgg cgauaagaaa     1740

aucacccacc acuggcauag auccggaagc acaauuggca aggcuuucga ggccaccgug     1800

cgcggggcua aacggauggc cguucuggga gacaccgcgu gggauuucgg cuccgucggg     1860

ggaguguuua auucucucgg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc     1920

cuguucggag gcauguccug guuuucucaa auccugaucg ggacacuucu cguguggcug     1980

ggacugaaca cgaagaacgg cagcauuucc cucaccuguc uggcccuugg gggaguuaug     2040

aucuuccuga gcacagcugu gucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4569
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 1 opt

<400>  4569
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4570
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 1 opt

<400>  4570
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucgugaacg acacggggua cgagacagau gaaaauagag ccaaggucga ggugaccccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaacag gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caaacacugg     1200

cucguccaua aggagugguu ccacgauauu ccucugcccu ggcacgcggg cgccgacaca     1260

gggacgccuc auuggaauaa caaagaagcu cugguggagu uuaaggaugc ccacgccaaa     1320

cgccagaccg uugugguccu cggaagccag gaaggcgcug ugcauacagc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggaaagcugu ucuccggcca ccucaaaugc     1440

cggcugaaga uggauaaacu uagacugaag gggguuagcu auucucucug uaccgcugcc     1500

uuuacauuca ccaaagugcc cgcugaaacg cugcauggaa ccgucacagu ggaggugcag     1560

uacgccggca ccgacgggcc gugcaagauc ccuguucaaa uggcggucga caugcagaca     1620

cugacccccg ugggacgccu cauuacggcc aauccuguga ucacagaauc caccgagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu cauuggggug     1740

ggcgauaaaa agaucacaca ccacuggcau cgguccggaa gcaccauugg caaggcuuuc     1800

gaggccaccg uuagaggggc uaaacgcaug gccgugcugg gagacacagc gugggauuuc     1860

ggcuccgucg ggggaguguu uaacucucuc ggcaaaggga uccaccagau uuucggcgcc     1920

gcuuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauccugau cgggacgcuu     1980

cucguuuggc ugggacugaa uaccaagaac ggcagcauuu cccucacaug ucuggcccuu     2040

gggggaguga ugaucuuccu gagcaccgcu gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4571
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 1 opt

<400>  4571
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucaca gaaagaaagg agaggcccgg cgcagcagac gggcugugac ccuuccgucc      360

cauucuacac gcaagcugca aaccagaagc cagacauggc ucgaaucccg ggaguauacc      420

aaacaccuga ucaagguuga gaacuggauc uuucgcaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu agaugcaucg gggugucuaa ccgggacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcaugg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgcuc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucuagauguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaacgga cacucgucga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauauag aauuaugcug uccguucacg gaagccaaca cucuggcaug     1020

aucgugaacg acacggggua cgagacagau gaaaaucggg ccaaggucga ggugaccccu     1080

aacagcccga gagccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

ccccgcacag gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caaacauugg     1200

cucguccaca aggagugguu ccaugauauu ccucugcccu ggcacgcggg cgccgacaca     1260

gggacgccuc acuggaauaa caaagaagcu cugguggagu uuaaggaugc ccaugccaaa     1320

cggcagaccg uugugguccu cggaagccag gaaggcgcug ugcacacagc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggaaagcugu ucuccggcca ucucaaaugc     1440

agacugaaga uggauaaacu ucgccugaag gggguuagcu auucucucug uaccgcugcc     1500

uuuacauuca ccaaagugcc cgcugaaacg cugcacggaa ccgucacagu ggaggugcag     1560

uacgccggca ccgacgggcc gugcaagauc ccuguucaaa uggcggucga caugcagaca     1620

cugacccccg ugggacggcu cauuacggcc aauccuguga ucacagaauc caccgagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu cauuggggug     1740

ggcgauaaaa agaucacaca ucacuggcac agauccggaa gcaccauugg caaggcuuuc     1800

gaggccaccg uucgcggggc uaaacggaug gccgugcugg gagacacagc gugggauuuc     1860

ggcuccgucg ggggaguguu uaacucucuc ggcaaaggga uccaucagau uuucggcgcc     1920

gcuuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauccugau cgggacgcuu     1980

cucguuuggc ugggacugaa uaccaagaac ggcagcauuu cccucacaug ucuggcccuu     2040

gggggaguga ugaucuuccu gagcaccgcu gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4572
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 1 opt

<400>  4572
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

acggugaacg acaucgggua cgagacagau gaaaauagag ccaaggucga ggugaccccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaacag gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caaacacugg     1200

cucguccaua aggagugguu ccacgauauu ccucugcccu ggcacgcggg cgccgacaca     1260

gggacgccuc auuggaauaa caaagaagcu cugguggagu uuaaggaugc ccacgccaaa     1320

cgccagaccg uugugguccu cggaagccag gaaggcgcug ugcauacagc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggaaagcugu ucuccggcca ccucaaaugc     1440

cggcugaaga uggauaaacu uagacugaag gggguuagcu auucucucug uaccgcugcc     1500

uuuacauuca ccaaagugcc cgcugaaacg cugcauggaa ccgucacagu ggaggugcag     1560

uacgccggca ccgacgggcc gugcaagauc ccuguucaaa uggcggucga caugcagaca     1620

cugacccccg ugggacgccu cauuacggcc aauccuguga ucacagaauc caccgagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu cauuggggug     1740

ggcgauaaaa agaucacaca ccacuggcau cgguccggaa gcaccauugg caaggcuuuc     1800

gaggccaccg uuagaggggc uaaacgcaug gccgugcugg gagacacagc gugggauuuc     1860

ggcuccgucg ggggaguguu uaacucucuc ggcaaaggga uccaccagau uuucggcgcc     1920

gcuuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauccugau cgggacgcuu     1980

cucguuuggc ugggacugaa uaccaagaac ggcagcauuu cccucacaug ucuggcccuu     2040

gggggaguga ugaucuuccu gagcaccgcu gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4573
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 1 opt

<400>  4573
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga uuaaggugga gaacuggauc uuucggaauc ccggcuucac gcuggucgcc      480

guggcgaucg ccuggcuccu gggcagcucu acaucccaga agguuauuua ccuugugaug      540

auccugcuca uugcuccugc cuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gaccugggug gaugucguuc uggagcacgg aggcugugug      660

acagugaugg cucaggacaa acccaccguc gauaucgaac uugugaccac aacgguuagc      720

aauauggccg aggugcgguc uuacugcuac gaggcgucca uuagcgacau ggccuccgau      780

ucucgcuguc cuacccaagg ggaggcuuau cuggacaagc agagcgacac acaguacguc      840

ugcaaaagaa cccucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua caugcgccaa auuuaccugc agcaagaaga ugacggggaa aucuauccag      960

cccgaaaauc ucgaauaucg caucaugcug uccgugcacg gaagccaaca uucuggcaug     1020

auugucaacg acgagaauag agcuaaggug gaagucacac cuaacagccc gcgggccgag     1080

gccacccuug ggggauucgg cucccugggc cucgauugug agcccagaac cgggcuggac     1140

uuuucugauc uuuacuaucu gacaaugaau aacaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaca ucccucugcc cuggcacgcu ggagcggaua ccggcacacc ucauuggaau     1260

aacaaagagg cccugguuga auuuaaggac gcucacgcca aacgccagac gguggucgug     1320

cucgggagcc aggagggagc cguucauacc gcucuggccg gcgcgcuuga agcugagaug     1380

gauggggcca agggccggcu guucuccgga caccucaagu gcagacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucucuc uguacagccg cuuuuaccuu cacaaagguc     1500

cccgccgaga cccugcaugg gacggugacc guggaaguuc aguaugcugg aacagauggc     1560

ccgugcaaag ucccugccca aauggcggug gacaugcaga cccugacacc cguggggcgg     1620

cucauuaccg ccaauccugu caucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugacccuc ccuuuggaga uucuuacauu gugaucggcg uuggggacaa gaaaaucacc     1740

caccacuggc auagauccgg cagcacaauu ggaaaggcuu ucgaagccac cgugcgcggc     1800

gcuaaacgga uggccguccu gggggauacc gcgugggacu ucggauccgu gggcgggguu     1860

uuuaacucuc ucggaaaggg cauccaccag auuuucgggg ccgcuuuuaa aagccuguuc     1920

ggcggaaugu ccugguuuuc ucaaauccug auuggcacac uucucgugug gcuggggcug     1980

aauacgaaga acggaagcau cucccucacc ugucuggccc uuggcggggu caugaucuuc     2040

cugagcacag cugugucugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4574
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 1 opt

<400>  4574
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucggguacg agacggacga aaacagagcc aagguggagg ucacaccuaa uagcccgcgg     1080

gccgaggcua cccuuggagg cuucggcucc cuggggcucg auugugaacc cagaaccgga     1140

cuggacuuuu cugaucuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag     1200

gagugguucc acgacauucc ucugcccugg cacgcgggcg ccgauaccgg gacaccucau     1260

uggaacaaua aagaagcucu ggucgaguuu aaggacgccc acgccaaacg ccagacggug     1320

guugugcucg gaagccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggaug gcgccaaggg aaagcuguuc uccggccacc ucaaaugccg gcugaagaug     1440

gacaaacuua gacugaaggg ggugagcuau ucucucugua cagcugccuu uaccuucaca     1500

aaaguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gaugggccgu gcaagauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacgccuca uuaccgccaa cccugugauc acggaaucca cagagaauag caagaugaug     1680

cuggaacuug acccucccuu uggcgauucu uacaucguga uuggggucgg cgacaaaaag     1740

aucacccacc acuggcaucg guccggaagc acaauuggca aggcuuucga ggccaccgug     1800

agaggggcua aacgcauggc cguucuggga gauaccgcgu gggacuucgg cuccgucggg     1860

ggaguguuua acucucucgg caaagggauc caccagauuu ucggcgccgc uuuuaagagc     1920

cuguucggag gcauguccug guuuucucaa auccugaucg ggacacuucu cguguggcug     1980

ggacugaaca cgaagaaugg cagcauuucc cucaccuguc uggcccuugg gggaguuaug     2040

aucuuccuga gcacagcugu gucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4575
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 1 opt

<400>  4575
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggccgcggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaaaaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4576
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 1 opt

<400>  4576
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggccuggc ucucgccgcg      480

gccgcuaucg ccuggcugcu uggcuccagc acgucucaga aagucaucua ucuggugaug      540

auucuccuga ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuc      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uugaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uggugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cucgacaagc agucugacac ccaguauguu      840

ugcaaacgga cacuggugga ucgcggaugg ggcaauggcu gugggcuguu uggaaagggc      900

agccucguga ccugcgccaa auucgccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc uggaguacag aaucaugcuu ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuggga ggcuuuggca gccucgggcu ggacugugag     1140

ccucgcaccg gacuugauuu cuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ucacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cucguggagu ucaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu gggaucucag gaaggcgcgg ugcauaccgc ucuugccggg     1380

gcccuggagg cugaaaugga cggcgccaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gcggcucaaa ggggucagcu auucucugug uacagcugcc     1500

uuuaccuuca caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cucacaccug ugggaagacu gaucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggaucccccu uuuggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccucg gagauaccgc cugggacuuu     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuucaaaa gccuguuugg aggcaugucc ugguucucuc aaauucucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggaguuc ugauuuuucu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4577
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 1 opt

<400>  4577
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacugguau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaucagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4578
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 1 opt

<400>  4578
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga cacccagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa caccaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcacagcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4579
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 1 opt

<400>  4579
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg agauccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4580
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 1 opt

<400>  4580
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacaggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cuggcucaaa uggccgugga caugcagacc     1620

cucacacccg ugggaagacu gaucaccgcu aacccuguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggauccgccc uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccucg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuuuucgg aggcaugucc ugguuuucuc aaauucucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4581
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 1 opt

<400>  4581
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuacccu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccguug ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa caccaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggagugc ucauuuuccu gagcacagcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4582
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 1 opt

<400>  4582
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgug gucucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4583
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 1 opt

<400>  4583
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccgcuugggu cguguauggg      300

accugucauc acaaaaaggg agaagccaga cggucucgca gagcuguuac gcuucccagc      360

cauuccaccc ggaaacugca aacacgcccu cagaccuggc ucgagucuag agaguacaca      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc ccggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcagcucc accagccaga aagucaucua ucuugugaug      540

auucugcuca ucgccccugc guacucuauu agaugcaucg ggguuuccaa ucgggacuuu      600

guggagggaa ugagcggcgg gacguggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cccaggacaa gcccaccgug gauauugagc uugugacaac caccgucucu      720

aacauggcug aggugcgcuc cuacugcuau gaagccagca ucucugacau ggcuuccgau      780

agcagauguc cuacacaagg ggaggccuac cuggacaagc aguccgacac gcaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

ucucugguca caugcgccaa auuugcuugc agcaagaaaa ugaccgggaa guccauucag      960

ccggaaaacc ucgaguacag aaucaugcug agcgugcacg gaucucaaca cuccggcaug     1020

aucguuaaug acacagggca ugaaaccgau gagaaccggg cgaaggugga gauuacgccc     1080

aauagcccua gagccgaagc uacacuugga ggcuucggcu cucuggggcu cgacugugag     1140

ccccgcaccg gacuggauuu uagcgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccucugcccu ggcacgccgg cgccgacaca     1260

gggaccccgc auuggaacaa uaaagaggcu cugguggaau uuaaggaugc ccacgcgaaa     1320

cggcagacag ucgugguucu cggaucccag gagggcgcug ugcauacggc ccuggccggg     1380

gcucuugagg ccgaaaugga cggcgcuaag ggaagacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucuccu auagccucug uaccgccgcg     1500

uucacauuua ccaagauucc ugccgagaca cugcauggaa ccgugacggu ugaagugcag     1560

uacgcuggca ccgacgggcc cugcaaaguc ccugcccaaa uggcugugga caugcagaca     1620

cugacccccg ugggaagacu caucacagcc aaccccguua uuaccgaguc uacggaaaac     1680

uccaagauga ugcuggagcu ugauccuccg uucggcgaca gcuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcucuggau ccaccaucgg caaggcguuu     1800

gaagccacag ugagaggggc uaaacggaug gccguccugg gagauaccgc uugggacuuc     1860

ggcagcguug ggggagcccu caauucccug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcucuauca gccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu guccacggcc gugagcgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4584
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 1 opt

<400>  4584
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucuaaaa aggucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaggg ggaggcuuac cuggacaaac aaucugacac ccaguauguu      840

ugcaagcgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaaaggc      900

agccugguga ccugcgccaa guuugccugc uccaaaaaga ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccagca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaagugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaagcacugg     1200

cucguccaua aagaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaggaggcc cugguggaau uuaaagaugc ucacgccaag     1320

cggcaaacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaaa ggaagacuga gcuccggcca ccucaagugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaaaauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaagguc cccgcucaga uggccgugga caugcaaacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaga agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaagccuuu     1800

gaagcgaccg ugagaggggc caagcggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaaaggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc agauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaaaaau ggcagcaucu cccugaugug ucuugcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4585
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 1 opt

<400>  4585
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgagacaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacucuauu cggugcaucg ggguuuccaa ucgcgacuuu      600

guggagggaa ugagcggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucu      720

aacauggccg aggugagauc cuacugcuau gaagcuagca ucucugacau ggccuccgau      780

agccgguguc ccacgcaagg ggaggcuuac cuggacaagc aguccgacac ccaguauguu      840

ugcaaacgca cacucgugga uagaggaugg ggcaauggcu gugggcuguu cggaaagggc      900

ucucugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa guccauucag      960

ccugaaaacc ucgaguaccg gaucaugcug agcgugcacg gaucucaaca cuccggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaacagag cuaaggugga gauuacaccg     1080

aauagccccc gcgcggaagc cacccuugga ggcuucggcu cucuggggcu cgacugugag     1140

ccucggaccg gacuggauuu uagcgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

agacagacgg ucgugguucu cggaucccag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggacgccugu cuagcggcca ccucaaaugc     1440

cggcugaaga uggauaagcu gagacugaaa ggggucuccu auagccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggacgccu caucaccgcu aaccccguua uuacggaguc uacagaaaac     1680

uccaagauga ugcuggagcu ugaucccccu uucggcgaca gcuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cggucuggau ccacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacgcaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcagcgucg ggggagcucu caauucccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcucuauca gccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4586
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 1 opt

<400>  4586
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaacccag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucuccu auagccucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc uaccgaaaac     1680

uccaagauga ugcuggagcu ugaucccccu uucggcgaca gcuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcucuggau ccaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcagcgucg ggggagcucu caauucccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa caccaagaau ggcucuauca gccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu guccacagcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4587
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 1 opt

<400>  4587
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacucccug agaugcauug ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uugaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauaucgagc uggugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cucgacaagc agucugacac ccaguauguu      840

ugcaaacgga cacuggugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccucguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc uggaguacag aauuaugcuu ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gaucacaccc     1080

aauucuccga gagcggaagc cacccuggga ggcuucggca gccucgggcu ggacugugag     1140

ccucgcaccg gacuugauuu uuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cucguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu gggaucucag gagggcgcgg ugcauaccgc ucuugccggg     1380

gcccuggagg cugaaaugga cggcgccaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gcggcucaaa ggggucagcu auucucugug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cucacaccug ugggaagacu gauuaccgcu aaccccguua ucacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggaucccccu uucggcgacu cuuacauugu caucggggug     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccucg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccucau ugggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggaguuc ugaucuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4588
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 1 opt

<400>  4588
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccggauuga aaauuggauc uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagugauuua ucuugucaug      540

auccugcuca uugcucccgc cuacuccauc agaugcauug gggugagcaa ucgggacuuu      600

guugagggaa ugucuggcgg gacaugggug gaugucgugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4589
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 1 opt

<400>  4589
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu cugcgcaacc cuggcuuugc ucucgccgcg      480

gccgcuaucg ccuggcugcu uggcuccagc acgucucaga aagucaucua ucuggugaug      540

auucuccuga ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuc      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uugaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uggugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cucgacaagc agucugacac ccaguauguu      840

ugcaaacgga cacuggugga ucgcggaugg ggcaauggcu gugggcuguu uggaaagggc      900

agccucguga ccugcgccaa auucgccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc uggaguacag aaucaugcuu ucuguccacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuggga ggcuuuggca gccucgggcu ggacugugag     1140

ccucgcaccg gacuugauuu cuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ucacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cucguggagu ucaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu gggaucucag gaaggcgcgg ugcauaccgc ucuugccggg     1380

gcccuggagg cugaaaugga cggcgccaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gcggcucaaa ggggucagcu auucucugug uacagcugcc     1500

uuuaccuuca caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cucacaccug ugggaagacu gaucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggaucccccu uuuggcgacu cuuacaucgu caucgggauu     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcucg gagauaccgc cugggacuuu     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuucaaaa gccuguuugg aggcaugucc ugguucucuc aaauccucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcauuu cccuuaugug ucuggcucuc     2040

gggggagugc ugaucuuucu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4590
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 1 opt

<400>  4590
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uuguguccac caccgucagc      720

aacauggccg aggugcgcuc uuacugcuau gaagcuacaa ucuccgacau ugccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auuguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gaucacaccg     1080

aauagcccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg cggucgugcu cggaucucag gagggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgaaaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaagccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4591
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 1 opt

<400>  4591
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaacugcag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cucacaccug ugggaagacu gaucaccgcu aaccccguga uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggaucccccu uucggcgacu cuuacaucgu uaucgggguc     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcucg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuuuucgg aggcaugucc ugguuuucuc aaauucucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggagugc ugauuuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4592
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 1 opt

<400>  4592
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc caucgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu cauuggggug     1740

ggcgagaaaa agaucacaca ccacuggcau cgcuccggaa gcaccauugg caaggccuuu     1800

gaagcgacag ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggaguuc ucaucuuccu gagcacggcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4593
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 1 opt

<400>  4593
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgucaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggugagcu auucucucug uacagccgcu     1500

uucaccuuua caaagauucc ggccgagacc cugcauggaa cgguuaccgu ggaaguccag     1560

uacgcgggca cagacgggcc uugcaaagug cccgcccaaa uggcugugga caugcagacc     1620

cugacaccug uuggaagacu caucaccgcc aaccccguca uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu gaucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggcuuuu     1800

gaagccaccg ucagaggggc gaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccguug ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu ccuucaugug ucuggcccuu     2040

gggggagugc ugauuuuucu cagcaccgcu gucucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4594
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 1 opt

<400>  4594
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaaaacuga gcuccggcca ccucaagugc     1440

agacugaaga uggauaaacu ucgccugaag ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaaaauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaagguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggacggcu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaga agauuaccca ccacuggcau agauccggaa gcacaaucgg caaagccuuu     1800

gaagcgaccg ugcgcggggc caagcggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaaaggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaaaaau ggcagcaucu cccugaugug ucuugcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4595
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 1 opt

<400>  4595
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caaggaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaca aaugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau ccaccugggu cguguauggg      300

acgugucauc acaagaaagg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaagcugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aaacaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aggucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ccgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aauauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaaac agucugacac ccaguauguu      840

ugcaagcgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaaaggc      900

agccugguga ccugcgccaa guuugccugc uccaaaaaga ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaagugga gauuacaccg     1080

aacucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaauaa caagcacugg     1200

cucguccaua aagaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaggaggcc cugguggaau uuaaagaugc ucacgccaag     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaaa ggaagacuga gcuccggcca ccucaagugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaaaauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaagguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaucccguua uuacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaga agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaagccuuu     1800

gaagcgaccg ugagaggggc caagcggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caacucucug ggcaaaggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaaaaac ggcagcaucu cccugaugug ucuugcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4596
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 1 opt

<400>  4596
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacaggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaggc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugaag cugagaugga uggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggacaaacu ucggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggaaacc cugcauggaa cggugaccgu ugaggugcag     1560

uacgccggca cagaugggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaauc cacagagaac     1680

agcaagauga ugcuggagcu ugacccgccu uucggcgauu cuuacaucgu caucggggug     1740

ggcgaaaaga agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaggcgaccg ugagaggggc caaacggaug gcuguccugg gagacaccgc cugggauuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4597
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 1 opt

<400>  4597
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacaggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccca gagcggaagc cgcucuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgccgg cgccgacaca     1260

gggaccccgc auuggaacaa uaaagaggcu cugguggaau uuaaggaugc ccacgcgaaa     1320

cggcagacag ucgugguucu cggaucucag gagggcgcug ugcauacggc ccuggccggg     1380

gcucuugagg ccgaaaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgccgcg     1500

uucacauuua ccaagauucc ggccgagaca cugcauggaa ccgugacggu ugaagugcag     1560

uacgcuggca ccgacgggcc uugcaaaguc cccgcccaaa uggcugugga caugcagaca     1620

cugaccccug ugggaagacu caucacagcc aaccccguua uuaccgaguc cacggaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggcguuu     1800

gaagccacag ugagaggggc uaaacggaug gccguccugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaacccuuau ugggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggaguuc ucaucuuccu gagcaccgcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4598
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 1 opt

<400>  4598
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggcg cugacgggcc uugcaaaguc cccgcccaaa uggcugugga caugcagaca     1620

cugaccccug ugggaagacu caucacagcc aaccccguua uuaccgaguc cacggaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggcguuu     1800

gaagccacag ugagaggggc uaaacggaug gccguccugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggaguuc ucauuuuccu gagcacggcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4599
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 1 opt

<400>  4599
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gccccaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucuggc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uugaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gcccacagug gauauugagc uggugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc cuacgcaagg ggaggcuuac cucgacaagc agucugacac ccaguauguu      840

ugcaaacgga cacuggugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccucguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

cccgaaaacc uggaguacag aaucaugcuu ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccu     1080

aauucuccga gagcggaagc cacccuggga ggcuucggca gccucgggcu ggacugugag     1140

ccccgcaccg gacuugauuu uuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccucugcccu ggcacgcugg cgccgacacc     1260

gggacaccuc auuggaacaa uaaagaggcc cucguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu gggaucucag gagggcgcgg ugcauaccgc ucuugccggg     1380

gcccuggagg cugaaaugga cggcgccaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gcggcucaaa ggggucagcu auucucugug uacagcugcc     1500

uucaccuuua caaagauucc cgcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc gugcaaaguc ccugcucaaa uggccgugga caugcagacc     1620

cucacacccg ugggaagacu gaucaccgcu aacccuguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggauccuccc uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccucg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4600
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 1 opt

<400>  4600
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau gguuagcgau      780

uccagauguc ccacgcaagg ggaggccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucgucga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgcuaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguucacg gaucccaaca cagcggcaug     1020

aucgugaaug acaccgggca ugaaacggau gagaaccggg ccaaggucga gauuacaccc     1080

aauucuccga gagcugaagc gacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauc ccccugccuu ggcacgccgg cgcugacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ccacgcuaaa     1320

cggcagacgg ugguugugcu cggaucucag gagggcgccg uccauaccgc gcuggcuggg     1380

gcccuugagg ccgaaaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcu     1500

uucaccuuua caaagauucc ggccgagacc cugcauggaa cgguuaccgu ggaaguccag     1560

uacgcgggca cagacgggcc uugcaaagug cccgcccaaa uggcugugga caugcagacc     1620

cugacaccug uuggaagacu caucaccgcc aaccccguca uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu gaucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggcuuuu     1800

gaagccaccg ucagaggggc gaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccguug ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcccuu     2040

gggggagugc ucauuuuccu gagcaccgcu gucucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4601
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 1 opt

<400>  4601
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggauuagcaa ucgggacuuu      600

guugagggaa ugucuggcgg gacaugggug gaugucgugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca uucuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uagcgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucccag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa gggguguccu auagccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguga ucacggaguc uacagaaaac     1680

uccaagauga ugcuggagcu ugaucccccu uucggcgaca gcuacauugu uaucgggguc     1740

ggcgagaaaa agauuaccca ccacuggcau cgcucuggau ccacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcagcgucg ggggagcucu caauucccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauccuuau ugggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcucuauca gccugaugug ucuugcucuu     2040

gggggagugc ucauuuuccu guccaccgcg guuagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4602
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 1 opt

<400>  4602
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaucc ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uucgggugga aaauuggauc uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucauuua ucuugugaug      540

auccugcuca uugcucccgc cuacuccauc agaugcauug ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauaucgagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

auuguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguua ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu cauuggggug     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cgcuaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggaguuc ucaucuuccu gagcacggcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4603
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 1 opt

<400>  4603
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcucaa agaucucggc gcgccguuac ccuucccagc      360

cauuccacaa gaaaacugca gacccggucu cagacauggc ucgagagccg cgaguacacc      420

aagcaccuga ucagagugga aaauuggauu uuucggaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu cgcugcaucg ggguuagcaa uagagacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaagacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcggag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

ucccgcuguc ccacgcaggg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaaagaa cacucgugga ucggggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucaa      960

ccugaaaacc ucgaguaccg caucaugcug ucugugcacg gaucccagca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaacagag cuaaggugga gauuacaccc     1080

aauucuccgc gggcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccuagaaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cgccagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggacggcuga gcuccggcca ccucaaaugc     1440

agacugaaga uggauaagcu gcgccugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcaa     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaga uggccgugga caugcagacc     1620

cugacaccug ugggacggcu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau agauccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugcgcggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccaaau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4604
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 1 opt

<400>  4604
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca agcucgcucu cagaccuggc ucgagagcag agaguacaca      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc accucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacguggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cccaggacaa gccuaccgug gauauugagc uugugacaac caccgucucc      720

aacauggcug aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacacaagg ggaggccuac cuggacaagc agucugacac gcaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugcuugc uccaagaaaa ugaccggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acacagggca ugaaaccgau gagaaccggg cgaaggugga gauuacgccc     1080

aauucuccca gagccgaagc uacacuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgccgg cgccgacaca     1260

gggaccccgc auuggaacaa uaaagaggcu cugguggaau uuaaggaugc ccacgcgaaa     1320

cggcagacag ucgugguucu cggaucucag gagggcgcug ugcauacggc ccuggccggg     1380

gcucuugagg ccgaaaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgccgcg     1500

uucacauuua ccaagauucc ggccgagaca cugcauggaa ccgugacggu ugaagugcag     1560

uacgcuggca ccgacgggcc uugcaaaguc cccgcccaaa uggcugugga caugcagaca     1620

cugaccccug ugggaagacu caucacagcc aaccccguua uuaccgaguc cacggaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggcguuu     1800

gaagccacag ugagaggggc uaaacggaug gccguccugg gagauaccgc uugggacuuc     1860

ggcuccguug ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu gagcacggcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4605
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 1 opt

<400>  4605
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucagc      720

aauauggccg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccguccacg gaagccaaca cucuggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacagcccca gagcggaagc cacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaagccag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaaa ggggucucuu auucccucug uacagcugcc     1500

uucaccuuua gcaagauucc ggcggagaca cugcauggaa ccgugacgga agagguucag     1560

uacgccggca ccgacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagaca     1620

cugaccccug ugggaagacu caucacagcu aaucccguga uuaccgaauc uacggagaac     1680

uccaagauga ugcuggagcu ugaucccccu uucggcgaca gcuacaucgu uaucgggguc     1740

ggcgaaaaga agauuacaca ccacuggcau cgcuccggau cuaccaucgg caaggccuuu     1800

gaggcgacag ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcagcgucg ggggagcucu caacucccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa uacaaagaac ggcagcaucu cucugaugug ucuugcucuc     2040

gggggagugc ugauuuuccu guccacggcg guuagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4606
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 1 opt

<400>  4606
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucagc      720

aauauggccg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccguccacg gaagccaaca cucuggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacagcccca gagcggaagc cacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacaccgg cgcugacaca     1260

gggacgcccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg ucgugguucu cggaagccag gagggcgccg ugcauacagc gcuggcuggg     1380

gcccuugagg ccgaaaugga cggcgcuaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucucuu auucccucug uaccgccgcu     1500

uucacauuua ccaagauucc ggccgagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgcgggca ccgacgggcc uugcaaaguc cccgcccaaa uggcugugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcc aaucccguua uuacagaggg caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggggacu cuuacaucgu caucggcgug     1740

ggagagaaaa agauuacaca ccacuggcau cgcuccggca gcaccaucgg gaaggcuuuu     1800

gaagccaccg ugcggggagc gaaaagaaug gccguccugg gcgauacagc uugggacuuc     1860

ggguccgugg gaggcguucu caacucucug gggaagggca uucaccagau cuucggagcc     1920

gcuuuuaaaa gccuguucgg cgggaugucc ugguuuucuc aaauucuuau cggaacgcuc     1980

cugauguggc ugggccucaa uaccaagaac gggagcaucu cccugaugug ucuugcccug     2040

ggaggcgugc ucauuuuccu gagcacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4607
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 1 opt

<400>  4607
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucagc      720

aauauggccg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacagcccca gagcggaagc cacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaagccag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucucuu auucccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaucccguua uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcagcggau ccacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcucugucg ggggagcucu caacagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaagaac ggcuccauca gccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gucuaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4608
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 1 opt

<400>  4608
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggaaacgcc uacuauaugu accuugaccg cuccgaugcg      120

ggcgaagcua uuucuuuccc cacaaccaug gggaugaaua agugcuauau ccagauuaug      180

gaccugggac acauguguga ugccacaaug agcuacgagu gcccuaugcu cgacgaaggc      240

guggagccgg augacgucga uuguuggugc aacaccacau ccaccugggu gguuuauggg      300

acgugucauc acaaaaaggg agaagccaga cggucucgca gagcugugac ccugcccagc      360

cauuccacac ggaaacuuca aacccgcucu cagacauggc uggagagcag agaguacacc      420

aagcaccuca uccgggucga aaauuggauu uuucgcaacc cuggcuucgc ccuggcugcc      480

gcggccaucg cuuggcugcu cggcuccagc acgucucaga aagugaucua ucugguuaug      540

auucuucuga ucgcccccgc uuacuccauu agaugcaucg gggugagcaa ucgggacuuu      600

gucgagggaa ugucuggcgg gacaugggug gauguggucc ucgaacaugg aggcuguguu      660

accgugaugg cccaggacaa gccuacagug gauauugagc uggucaccac cacagugucc      720

aacauggcgg agguucgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggccuac cuugacaagc agucugacac ccaguaugug      840

ugcaaacgga cacuggucga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgcuaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc uggaguacag aaucaugcuc ucuguucacg gaucccaaca cagcggcaug     1020

aucgugaaug acaccgggca ugaaacggau gagaaccggg ccaaggucga gauuacaccg     1080

aauagcccca gagcugaagc gacccuggga ggcuucggca gccuugggcu ggacugugag     1140

ccucgcaccg gacucgauuu uuccgaccug uauuaccuua caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccccucccuu ggcacgccgg cgcugacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ccacgcuaaa     1320

cggcagacgg ugguugugcu gggaucucag gagggcgccg uccauaccgc gcucgcuggg     1380

gcccuggagg ccgaaaugga cggcgcuaag ggaagacuua gcuccggcca ccugaaaugc     1440

cgccucaaga uggauaagcu gcggcuuaaa ggggugagcu auucucugug uacagccgcu     1500

uucaccuuua caaagauucc ggccgagacc cuccauggaa cgguuaccgu ggaaguccag     1560

uacgcgggca cagacgggcc uugcaaagug cccgcccaaa uggcugugga caugcagacc     1620

cugacaccug uuggaagacu gaucaccgcc aaccccguca uuacggaguc cacagaaaac     1680

agcaagauga ugcucgagcu ggaucccccu uucggcgacu cuuacaucgu gaucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggcuuuu     1800

gaagccaccg ucagaggggc gaaacggaug gccgugcuug gagauaccgc uugggacuuc     1860

ggcuccguug ggggagcccu caauucucuc ggcaagggga uucaccagau cuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucugau cgggacacuu     1980

cucguguggc ugggacugaa cacgaagaau ggcagcaucu cccucaugug ucuggcccuu     2040

gggggagucc ugauuuuccu cagcaccgcu gugucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4609
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 1 opt

<400>  4609
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucagc      720

aauauggccg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacagcccca gagcggaagc cacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaagccag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucucuu auucccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaucccguua uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcagcggau ccacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcucugucg ggggagcucu caacagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaagaac ggcuccauca gccugguuug ucuugcucuu     2040

gggggagugc ucauuuuccu gucuaccgcg gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4610
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 1 opt

<400>  4610
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuaacaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugguuug ucuugcucuu     2040

gggggagugc ucauuuuccu gagcaccgcg gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4611
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 1 opt

<400>  4611
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggauccgcc uacuauaugu accuugaccg cucugaugcg      120

ggcgaagcua uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgucga uuguuggugc aauaccacau cuaccugggu gguuuauggg      300

acgugucauc acaaaaaggg agaagccaga cggagccgca gagcugugac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggucga aaacuggauu uuucgcaauc cuggcuucgc ccuggcugcc      480

gcggccaucg cuuggcuccu gggcagcucu acgucccaga aagugaucua ucuuguuaug      540

auucugcuca ucgcccccgc uuacagcauu agaugcaucg gggugucuaa ccgggacuuu      600

gucgagggaa uguccggcgg gacaugggug gauguggucc uggaacaugg aggcuguguu      660

accgugaugg cccaggacaa gccugcggug gauauugagc uugucacaac caccgugagc      720

aauauggccg agguucgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacacaagg ggaggcuuac cuggacaagc agagcgacac gcaguaugug      840

ugcaaacgga cccucgucga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga caugcgccaa auuugccugc agcaagaaaa ugaccgggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccguucacg gaagccaaca cucuggcaug     1020

aucgugaacg acacagggca ugaaaccgau gagaaucggg cuaaggucga gauuacgccg     1080

aacagcccca gagcggaagc cacacuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caaacacugg     1200

cucgugcaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacacccc auuggaauaa caaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ugguugugcu cggaagccag gagggcgcgg uccauacggc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugucuu auucccucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagaca cugcauggaa ccguuacggu ggaaguccag     1560

uacgccggca ccgacgggcc uugcaaagug cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug uuggaagacu caucacagcu aaucccguca uuaccgagag cacggaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu gaucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcagcggau ccaccaucgg caaggccuuu     1800

gaagcgacag ucagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcucuguug ggggagcucu caacagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacccuc     1980

cugguguggc ugggacucaa uacaaagaac ggcuccauca gccugacgug ucuugcucuu     2040

gggggagucc ucauuuuccu gucuaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4612
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 1 opt

<400>  4612
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggauccgcc uacuauaugu accuugaccg cucugaugcg      120

ggcgaagcua uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgucga uuguuggugc aauaccacau cuaccugggu gguuuauggg      300

acgugucauc acaaaaaggg agaagccaga cggagccgca gagcugugac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggucga aaacuggauu uuucgcaauc cuggcuucgc ccuggcugcc      480

gcggugaucg ccuggcuccu gggcagcucu acgucccaga aaguuaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg gggucucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacaugggug gaugucguuc uggaacaugg aggcugugug      660

accgugaugg cucaggacaa gccuacaguc gauauugagc uugugaccac cacaguuagc      720

aauauggccg aggugcgcuc uuacugcuau gaagcgucca ucagcgacau ggccuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguc      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgccaa auuugcuugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucgucaacg acaccgggca ugaaacggau gagaaucggg ccaaggugga gauuacaccg     1080

aacagcccca gagccgaagc uacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcggg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcu cugguggaau uuaaggaugc ccacgccaaa     1320

cggcagacgg uugugguccu cggaagccag gagggcgcug ugcauaccgc ccuggcgggg     1380

gcucuugagg ccgaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa gggguuucuu auucccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcugagacc cugcauggaa cggugaccgu cgaagugcag     1560

uacgccggca cagacgggcc uugcaaagug cccgcgcaaa uggccguuga caugcagacc     1620

cugacaccug ucggaagacu caucaccgcu aaucccguga uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu gaucgggguc     1740

ggcgagaaaa agauuaccca ccacuggcau cgcagcggau ccacaaucgg caaggccuuu     1800

gaagcuaccg ugagaggggc caaacggaug gcgguucugg gagauaccgc cugggacuuc     1860

ggcucugucg ggggagcucu caacagccug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacacuc     1980

cugguguggc ugggacucaa uacgaagaac ggcuccauca gccugaccug ucuugcccuu     2040

gggggagugc ucauuuuccu gucuacagcc guuuccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4613
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 1 opt

<400>  4613
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggauccgcc uacuauaugu accuugaccg cucugaugcg      120

ggcgaagcua uuagcuuccc cacaacccug ggggugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

gucgagccgg augacgugga uuguuggugc aauaccacau cuaccugggu uguguauggg      300

acgugucauc acaaaaaggg agaagccaga cggagccgca gagcugucac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ccuggcugcc      480

gcggccaucg cuuggcuccu gggcagcucu acgucccaga aaguuaucua ucuugugaug      540

auucugcuca ucgcccccgc uuacagcauu agaugcaucg gggucucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacaugggug gaugucguuc uggaacaugg aggcugugug      660

accgugaugg cccaggacaa gccuacaguc gauauugagc uugugaccac cacaguuagc      720

aauauggcgg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggccuac cuggacaagc agagcgacac ccaguauguc      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgcuaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucgucaacg acaccgggca ugaaacggau gagaaucggg ccaaggugga gauuacaccg     1080

aacagcccca gagcugaagc gacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgccgg cgcugacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacgg uugugguccu cggaagccag gagggcgccg ugcauaccgc gcuggcuggg     1380

gcccuugagg ccgaaaugga cggcgcuaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa gggguuucuu auucccucug uacagccgcu     1500

uucaccuuua caaagauucc ggccgagacc cugcauggaa cggugaccgu cgaagugcag     1560

uacgcgggca cagacgggcc uugcaaagug cccgcccaaa uggcuguuga caugcagacc     1620

cugacaccug ucggaagacu caucaccgcc aaucccguga uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu gaucgggguc     1740

ggcgauaaaa agauuaccca ccacugguau cgcagcggau ccacaaucgg caaggcuuuu     1800

gaggccaccg ugagaggggc gaaacggaug gccguucugg gagacaccgc uugggauuuc     1860

ggcucugucg ggggagcccu caacagccug ggcaagggga uucaucagau cuucggcgcu     1920

gccuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacacuc     1980

cugguguggc ugggacucaa uacgaagaac ggcuccauca gccugaccug ucuugcccuu     2040

gggggagugc ucauuuuccu gucuacagcu guuuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4614
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 1 opt

<400>  4614
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggauccgcc uacuauaugu accuugaccg cucugaugcg      120

ggcaaggcua uuagcuuccc cacaacccug ggggugaaca aaugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgaau gcccuaugcu ggacgagggc      240

gucgaaccgg augacgugga uuguuggugc aauaccacau cuaccugggu uguguauggg      300

acgugucauc acaagaaagg agaggccaga cggagccgca gagcugucac ccuucccucc      360

cauucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguacacc      420

aaacaccuga uccgggugga gaacuggauu uuucgcaauc cuggcuucgc ccuggcugcc      480

gcggccaucg cuuggcuccu gggcagcucu acgucccaga agguuaucua ucuugugaug      540

auucugcuca ucgcccccgc uuacagcauu agaugcaucg gggucucuaa ccgggacuuu      600

guggaaggaa uguccggcgg gacaugggug gaugucguuc uggagcaugg aggcugugug      660

accgugaugg cccaggacaa gccuacaguc gauauugaac uugugaccac cacaguuagc      720

aauauggcgg aggugcgcuc uuacugcuau gaggccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaagccuac cuggacaaac agagcgacac ccaguauguc      840

ugcaagcgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaaaggc      900

ucccugguua ccugcgcuaa guuugccugc agcaaaaaga ugacagggaa gucuauucag      960

ccugagaauc ucgaauacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucgucaacg acauugggca ugagaccgau gaaaaucggg ccaaagugga gaucacgccg     1080

aacagcccca gagcugaggc gacacuugga ggcuucggcu cccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caagcacugg     1200

cucguccaua aagagugguu ccacgauauu ccccugccuu ggcacgccgg cgcugacaca     1260

gggacacccc auuggaauaa caaggaagcc cugguggagu uuaaagaugc ccacgcuaag     1320

cggcagacag uugugguccu cggaagccag gaaggcgccg ugcauacggc gcuggcuggg     1380

gcccuugagg ccgagaugga cggcgcuaaa ggaagacugu ccagcggcca ccucaagugc     1440

cgccugaaga uggauaaacu ucggcugaag gggguuucuu auucccucug uaccgccgcu     1500

uucacauuua ccaaaauccc ggccgaaaca cugcauggaa ccgugacggu cgaggugcag     1560

uacgcgggca ccgacgggcc uugcaaggug cccgcccaaa uggcuguuga caugcagaca     1620

cugaccccug ucggaagacu cauuacagcc aaucccguga ucaccgaaag cacggagaac     1680

ucuaaaauga ugcuggaacu ugaucccccu uucggcgacu ccuacaucgu gauugggguc     1740

ggcgauaaga agaucacaca ccacuggcau cgcagcggau ccaccauugg caaggcuuuu     1800

gaggccacag ugagaggggc gaaacggaug gccguucugg gagacaccgc uugggauuuc     1860

ggcucugucg ggggagcccu caacagccug ggcaaaggga uccaccagau uuucggcgcu     1920

gccuuuaagu cccuguucgg aggcaugucu ugguuuagcc aaauccuuau cgggacccuc     1980

cugguguggc ugggacucaa uaccaaaaac ggcuccauua gccugacgug ucuugcccuc     2040

gggggagugc ugaucuuccu gucuaccgcu guuuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4615
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 1 opt

<400>  4615
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga uuaaggugga gaacuggauc uuucggaauc ccggcuucac gcuggucgcc      480

guggcgaucg ccuggcuccu gggcagcucu acaucccaga agguuauuua ccuugugaug      540

auccugcuca uugcuccugc cuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gaccugggug gaugucguuc uggagcacgg aggcugugug      660

acagugaugg cucaggacaa acccaccguc gauaucgaac uugugaccac aacgguuagc      720

aauauggccg aggugcgguc uuacugcuac gaggcgucca uuagcgacau ggccuccgau      780

ucucgcuguc cuacccaagg ggaggcuuau cuggacaagc agagcgacac acaguacguc      840

ugcaaaagaa cccucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua caugcgccaa auuuaccugc agcaagaaga ugacggggaa aucuauccag      960

cccgaaaauc ucgaauaucg caucaugcug uccgugcacg gaagccaaca uucuggcaug     1020

auugucaacg acacagggca cgagaccgau gaaaauagag cuaaggugga ggucaccccu     1080

aacagcccgc gggccgaggc cacacuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaaccg gacuggauuu uucugaccuu uacuaucuga caaugaauaa caaacauugg     1200

cucgugcaca aggagugguu ccacgauauc ccucugcccu ggcaugcugg cgcggacacg     1260

gggaccccuc acuggaauaa caaagaagcc cugguugagu uuaaggaugc ucaugccaaa     1320

cgccagacag uggucgugcu cggaagccag gaaggcgccg uucacaccgc ucuggccggg     1380

gcgcuugagg cugagaugga cggcgccaag ggacggcugu ucuccggcca ucucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag ggggugagcu acucucucug uacagccgcu     1500

uuuaccuuca cgaagguccc cgccgaaacc cugcacggaa cagugaccgu ggagguucag     1560

uaugcuggca cagacgggcc gugcaaaguc ccugcccaaa uggcggugga caugcagacc     1620

cugacgcccg ugggacggcu cauuacagcc aauccuguca ucaccgaauc cacagagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacauugu gaucgggguu     1740

ggcgauaaga aaaucaccca ccauuggcac agauccggaa gcaccauugg caaggcuuuc     1800

gaggccacag ugcgcggggc uaaacggaug gccguccugg gagacacggc gugggauuuc     1860

ggcuccguug ggggaguguu uaacucucuc ggcaagggga uccaucagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacccuu     1980

cucguguggc ugggacugaa uacaaagaac ggcagcaucu cccucaccug ucuggcccuu     2040

gggggaguca ugaucuuccu gagcacagcu gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4616
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 1 opt

<400>  4616
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaaaccug ggggugaaua aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuaccaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aacacaaccu cuacaugggu ggucuacggg      300

accugucacc auaagaaagg agaggccaga cggagccgca gagcugugac gcuuccgucc      360

cacucuaccc ggaagcugca aacacgcagc cagaccuggc ucgaauccag agaguauaca      420

aaacaucuga ucaagguuga gaauuggauc uuucggaacc ccggcuucgc ccuggcggcc      480

guggcuauug ccuggcuccu gggcagcucu accucccaga aggucaucua ccuugugaug      540

auucugcuca ucgcuccugc cuauagcauu cgcugcaucg ggguuucuaa uagagacuuu      600

guggaaggaa uguccggcgg gacguggguc gauguggugc uggagcacgg aggcuguguc      660

acaguuaugg cgcaggacaa acccaccgug gauauugaac uugugacaac caccgucagc      720

aacauggccg aggugcgguc uuacugcuac gaggcuucca ucagcgacau ggccuccgau      780

ucucgcuguc cuacacaagg ggaggcuuau cuggacaagc agagcgacac gcaguacguu      840

ugcaaaagaa cccucgugga ucggggaugg ggcaauggcu gugggcuguu cggaaagggc      900

ucccugguca caugcgccaa auuuaccugc agcaagaaga ugacagggaa aucuauccag      960

cccgaaaacc ucgaauaucg cauuaugcug uccgugcacg gaagccaaca uucuggcaug     1020

aucguuaaug acauugggca cgagaccgau gaaaacagag ccaaggugga ggucacgccu     1080

aauagcccgc gggcugaggc gacacuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaaccg gacuggauuu uucugaccuu uacuaucuga ccaugaacaa uaaacauugg     1200

cucgugcaca aggagugguu ccacgauauc ccucugcccu ggcaugccgg cgcugacaca     1260

gggaccccuc acuggaacaa uaaagaagcc cuggucgagu uuaaggaugc ccaugcuaaa     1320

cgccagacag ugguugugcu cggaagccag gaaggcgccg uccacacggc gcuggcuggg     1380

gcccuugagg ccgagaugga cggcgcuaag ggacggcugu ucuccggcca ucucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag ggggugagcu acucucucug uaccgccgcu     1500

uuuacauuca ccaagguucc cgccgaaaca cugcacggaa ccgugacggu cgaggugcag     1560

uaugcgggca ccgacgggcc gugcaaagug ccugcccaaa uggcuguuga caugcagaca     1620

cugacccccg ucggacggcu cauuacagcc aacccuguga ucaccgaauc cacggagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu gauugggguc     1740

ggcgauaaga aaaucacaca ccauuggcac agauccggaa gcaccauugg caaggcuuuc     1800

gaggccacag ugcgcggggc gaaacggaug gccguucugg gagacaccgc uugggauuuc     1860

ggcuccgucg ggggaguguu uaauucucuc ggcaaggggg ugcaucagau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucugau cgggacccuu     1980

cucguuuggc ugggacugaa cacaaagaau ggcagcaucu cccucacgug ucuggcccuu     2040

gggggaguga ugauuuuccu gagcaccgcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4617
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 1 opt

<400>  4617
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucgugaacg acauugggca cgagacggau gaaaauagag ccaaggucga ggugacaccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaaccg gacuggauuu uucugaccuu uacuaucuga caaugaauaa caaacauugg     1200

cucguccaca aggagugguu ccacgauauc ccucugcccu ggcaugcggg cgccgacacc     1260

gggacaccuc acuggaauaa caaagaagcu cugguggagu uuaaggaugc ccaugccaaa     1320

cgccagacgg uugugguccu cggaagccag gaaggcgcug ugcacaccgc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggacggcugu ucuccggcca ucucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag gggguuagcu acucucucug uacagcugcc     1500

uuuaccuuca caaaggugcc cgcugaaacc cugcacggaa cggucaccgu ggaggugcag     1560

uaugccggca cagacgggcc gugcaaaguu ccugcgcaaa uggccgucga caugcagacc     1620

cugacacccg ugggacggcu cauuaccgcu aauccuguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu cauuggggug     1740

ggcgauaaga aaaucaccca ccauuggcac agauccggaa gcacaauugg caaggccuuc     1800

gaggcuaccg uucgcggggc caaacggaug gcggugcugg gagacaccgc cugggauuuc     1860

ggcuccgucg ggggaguguu uaacucucuc ggcaagggga uccaucagau uuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccugau cgggacacuu     1980

cucguuuggc ugggacugaa uacgaagaac ggcagcauuu cccucaccug ucuggcucuu     2040

gggggaguga ugaucuuccu gagcacagcc gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4618
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 1 opt

<400>  4618
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaaaccug ggggugaaua aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuaccaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aacacaaccu cuacaugggu ggucuacggg      300

accugucacc auaagaaagg agaggccaga cggagccgca gagcugugac gcuuccgucc      360

cacucuaccc ggaagcugca aacacgcagc cagaccuggc ucgaauccag agaguauaca      420

aaacaucuga ucaagguuga gaauuggauc uuucggaacc ccggcuucgc ccuggcggcc      480

guggcuauug ccuggcuccu gggcagcucu accucccaga aggucaucua ccuugugaug      540

auucugcuca ucgcuccugc cuauagcauu cgcugcaucg ggguuucuaa uagagacuuu      600

guggaaggaa uguccggcgg gacguggguc gauguggugc uggagcacgg aggcuguguc      660

acaguuaugg cgcaggacaa acccaccgug gauauugaac uugugacaac caccgucagc      720

aacauggccg aggugcgguc uuacugcuac gaggcuucca ucagcgacau ggccuccgau      780

ucucgcuguc cuacacaagg ggaggcuuau cuggacaagc agagcgacac gcaguacguu      840

ugcaaaagaa cccucgugga ucggggaugg ggcaauggcu gugggcuguu cggaaagggc      900

ucccugguca caugcgccaa auuuaccugc agcaagaaga ugacagggaa aucuauccag      960

cccgaaaacc ucgaauaucg cauuaugcug uccgugcacg gaagccaaca uucuggcaug     1020

aucguuaaug acgagaacag agccaaggug gaagucaccc cuaauagccc gcgggcugag     1080

gcgacgcuug ggggauucgg cucccugggc cucgauugug agcccagaac agggcuggac     1140

uuuucugauc uuuacuaucu gaccaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaca uuccucugcc cuggcacgcc ggagcugaua ccggcacacc ucauuggaac     1260

aauaaagagg cccuggucga auuuaaggac gcccacgcua aacgccagac cgugguugug     1320

cucgggagcc aggagggagc cguccauaca gcgcuggcug gcgcccuuga agccgagaug     1380

gauggggcua agggccggcu guucuccgga caccucaagu gcagacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucucuc uguacggccg cuuuuaccuu cacaaagguu     1500

cccgccgaga cccugcaugg gacagugacc gucgaagugc aguaugcggg aacggauggc     1560

ccgugcaaag ugccugccca aauggcuguu gacaugcaga cccugacacc cgucgggcgg     1620

cucaucaccg ccaacccugu gauuacagag uccaccgaaa acagcaagau gaugcuggag     1680

cuugacccuc ccuuuggaga uucuuacauc gugaucggcg ucggggacaa gaaaauuacg     1740

caccacuggc auagauccgg cagcacaauc ggaaaggcuu ucgaagccac cgugcgcggc     1800

gcgaaacgga uggccguucu gggggauaca gcuugggacu ucggauccgu gggcgggguc     1860

uuuaauucuc ucggaaaggg cauucaccag aucuucgggg ccgcuuuuaa aagccuguuc     1920

ggcggaaugu ccugguuuuc ucaaauucug aucggcaccc uucucgugug gcuggggcug     1980

aacaccaaga auggaagcau cucccucaca ugucuggccc uuggcggggu uaugauuuuc     2040

cugagcacgg ccgugucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4619
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 1 opt

<400>  4619
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga uuaaggugga gaacuggauc uuucggaauc ccggcuucgc ccuggucgcg      480

guggccaucg cuuggcuccu gggcagcucu acgucccaga agguuauuua ccuugugaug      540

auccugcuca uugccccugc uuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gacaugggug gaugucguuc uggagcacgg aggcugugug      660

accgugaugg cccaggacaa acccacaguc gauaucgaac uugugaccac cacaguuagc      720

aauauggcgg aggugcgguc uuacugcuac gaggccucca uuagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacguc      840

ugcaaaagaa cacucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg caucaugcug uccgugcacg gaagccaaca uucuggcaug     1020

auugucaacg acacggggca cgagacagau gaaaauagag ccaaggugga ggucaccccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaacag gacuggauuu uucugaccuu uacuaucuga ccaugaauaa caaacauugg     1200

cucgugcaca aggagugguu ccacgauauc ccucugcccu ggcaugcggg cgccgacaca     1260

gggacgccuc acuggaauaa caaagaagcu cugguugagu uuaaggaugc ccaugccaaa     1320

cgccagaccg uggucgugcu cggaagccag gaaggcgcug uucacacagc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggacggcugu ucuccggcca ucucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag ggggugagcu acucucucug uaccgcugcc     1500

uuuacauuca ccaagguccc cgcugaaacg cugcacggaa ccgugacagu ggagguucag     1560

uaugccggca ccgacgggcc gugcaaaguc ccugcgcaaa uggccgugga caugcagaca     1620

cugacccccg ugggacggcu cauuacggcu aauccuguca ucacagaauc caccgagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacauugu gaucgggguu     1740

ggcgauaaga aaaucacaca ccauuggcac agauccggaa gcaccauugg caaggccuuc     1800

gaggcuaccg ugcgcggggc caaacggaug gcgguccugg gagacacagc cugggauuuc     1860

ggcuccguug ggggaguguu uaacucucuc ggcaagggga uccaucagau uuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacgcuu     1980

cucguguggc ugggacugaa uaccaagaac ggcagcaucu cccucacaug ucuggcucuu     2040

gggggaguca ugaucuuccu gagcaccgcc gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4620
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 1 opt

<400>  4620
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucaac acaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cauucuacac ggaagcugca gacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaccuga uuaaggugga gaacuggauc uuucggaauc ccggcuucgc ccuggucgcg      480

guggccaucg cuuggcuccu gggcagcucu acgucccaga agguuauuua ccuugugaug      540

auccugcuca uugccccugc uuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gacaugggug gaugucguuc uggagcaugg aggcugugug      660

accgugaugg cccaagacaa acccacaguc gauaucgaac uugugaccac cacaguuagc      720

aauauggcgg aggugcgguc uuacugcuac gaggccucca uuagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaggg ggaagccuau cuggacaagc agagcgacac ccaguacguc      840

ugcaaaagaa cacucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccaa      960

cccgagaauc ucgaauaucg caucaugcug uccgugcacg gaagccagca cucuggcaug     1020

auugucaacg acaucgggca ugagacggau gaaaauagag ccaaggugga ggucacaccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaaccg gacuggauuu uucugaccuu uacuaucuga caaugaauaa caaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccucugcccu ggcacgcggg cgccgacacc     1260

gggacaccuc auuggaauaa caaagaagcu cugguugagu uuaaggaugc ccacgccaaa     1320

cgccagacgg uggucgugcu cggaagccag gaaggcgcug uucauaccgc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggacggcugu ucuccggcca ccucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag ggggugagcu acucucucug uacagcuguc     1500

ugcaccgccg cuaaggugcc cgccgaaaca cugcauggaa ccgugacggu ugagguccaa     1560

uaugcgggca ccgacgggcc guguaaagug ccugcccaga uggcugugga caugcagaca     1620

cugacccccg ucggacggcu caucacagcc aauccuguga uuaccgaauc cacggagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu uaucggggug     1740

ggcgauaaga aaauuacaca ccacuggcau agauccggaa gcaccaucgg caaggcuuuc     1800

gaggccacag uccgcggggc gaaacggaug gccgugcugg gagacaccgc uugggauuuu     1860

ggcuccguug ggggaguguu caacucucuc ggcaagggga uucaccaaau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauucugau cgggacccuu     1980

cucgucuggc ugggacugaa uacaaagaac ggcagcaucu cccucacgug ccuggcccuu     2040

gggggaguga ugauuuuccu gagcaccgcc guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4621
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 1 opt

<400>  4621
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga uuaaggugga gaacuggauc uuucggaauc ccggcuucgc ccuggucgcg      480

guggccaucg cuuggcuccu gggcagcucu acgucccaga agguuauuua ccuugugaug      540

auccugcuca uugccccugc uuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gacaugggug gaugucguuc uggagcacgg aggcugugug      660

accgugaugg cccaggacaa acccacaguc gauaucgaac uugugaccac cacaguuagc      720

aauauggcgg aggugcgguc uuacugcuac gaggccucca uuagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacguc      840

ugcaaaagaa cacucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg caucaugcug uccgugcacg gaagccaaca uucuggcaug     1020

auugucaacg acgagaauag agccaaggug gaagucacgc cuaacagccc gcgggccgag     1080

gcuacacuug ggggauucgg cucccugggc cucgauugug agcccagaac cgggcuggac     1140

uuuucugauc uuuacuaucu gaccaugaau aacaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaca ucccucugcc cuggcacgcg ggagccgaua caggcacccc ucauuggaau     1260

aacaaagagg cucugguuga auuuaaggac gcccacgcca aacgccagac aguggucgug     1320

cucgggagcc aggagggagc uguucauacg gcccuggcgg gcgcucuuga agccgagaug     1380

gauggggcca agggccggcu guucuccgga caccucaagu gcagacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucucuc uguaccgcug ccuuuacauu caccaagguc     1500

cccgcugaga cacugcaugg gaccgugacg guggaaguuc aguaugccgg aaccgauggc     1560

ccgugcaaag ucccugcgca aauggccgug gacaugcaga cacugacccc cguggggcgg     1620

cucauuacag cuaauccugu caucaccgag uccacggaaa acagcaagau gaugcuggag     1680

cuugacccuc ccuuuggaga uucuuacauu gugaucggcg uuggggacaa gaaaaucaca     1740

caccacuggc auagauccgg cagcaccauu ggaaaggccu ucgaagcuac agugcgcggc     1800

gccaaacgga uggcgguccu gggggauacc gccugggacu ucggauccgu gggcgggguu     1860

uuuaacucuc ucggaaaggg cauccaccag auuuucgggg cugccuuuaa aagccuguuc     1920

ggcggaaugu ccugguuuuc ucaaauccug auuggcaccc uucucgugug gcuggggcug     1980

aauacaaaga acggaagcau cucccucacg ugucuggcuc uuggcggggu caugaucuuc     2040

cugagcaccg ccgugucugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4622
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 1 opt

<400>  4622
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaaccug augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agagaccaga cggagccgca gauccguguc ucuucgguau      360

cacuacacac gcaagcugca aaccagaagc cagacauggc ucgaaucccg ggaguauaaa      420

aagcaucuga ucaugguuga gaacuggauc uuucgcaauc cgggcuucgc cauugugagc      480

gucgcuauca ccuggcugau gggcucucuc acgucccaga aggugauuua ccugguuaug      540

aucgugcuua uuguccccgc cuacagcauc ucuugcauug ggguguccaa cagagaccug      600

guggaaggaa ugagcggcgg gacauggguc gauguugugc ucgagcacgg aggcugugug      660

accgaaaugg cgcaggacaa accuacaguc gauaucgagc uggugaccau gaccguuucu      720

aauauggccg aggugcgguc cuauugcuac gaagcuagcc uuuccgacau ggccucugcu      780

agccgcuguc ccacacaagg ggagccuucc cuggauaagc agagcgacac gcagucuguc      840

ugcaaaagaa cccucggaga ccggggcugg ggcaacgggu guggaaucuu uggcaagggg      900

ucccugguga caugcagcaa auuuaccugc uguaagaaga ugcccggaaa aucuauucag      960

ccugaaaauc uggaguaucg caucaugcuc ccgguucacg gcagccaaca uuccgggaug     1020

auugugaacg auaucggaca cgaaacagac gagaauagag ccaaggucga ggugaccccc     1080

aacucuccuc gggcggaagc cacgcugggc ggcuuuggga gccuuggacu ggauugcgag     1140

cccagaacag gccucgacuu cuccgaucug uacuaucuua ccaugaauaa caaacauugg     1200

cugguccaca aggaaugguu ucacgacauu ccucuccccu ggcaugcugg ggccgauacc     1260

ggaacaccgc acuggaauaa caaagaggcu cugguggagu ucaaggacgc ccaugccaaa     1320

cgccagaccg uugugguccu gggcagccag gaaggggcug ugcacacagc gcucgccggc     1380

gcucuggagg ccgaaaugga uggagccaag ggccggcuuu ucucugggca ucugaagugu     1440

agacucaaaa uggacaagcu gcgccuuaaa ggaguuuccu acagccugug cacggcugcc     1500

uucaccuuua caaaggugcc ugcggagacc cuccacggca cagucaccgu ggaagugcag     1560

ucugcuggga cggauggacc cuguaaaguu ccugcccaaa uggccgucga caugcagacc     1620

cugacacccg ugggccggcu gaucaccgcu aaucccguga ucacagaguc caccgaaaac     1680

agcaagauga ugcucgagcu ggacccuccg uucggggauu ccuauauugu caucggcgug     1740

ggagacaaga aaauuacgca ccauuggcac agaucuggca gcacaaucgg gaaggccuuu     1800

gaggcuaccg uucgcggagc caaacggaug gcggugcuug gcgauacagc cugggacuuc     1860

ggguccgucg gaggcguguu caacucucug gggaagggca uucaucagau cuuuggagcu     1920

gccuucaaaa gccucuuugg cgggaugucc ugguucagcc aaauccugau uggaacccug     1980

cuuguuuggc ucggccugaa uaccaagaac gggucuaucu cccugacaug ccucgcucug     2040

ggaggcguga ugauuuuucu uagcacggcc gucucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4623
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 1 opt

<400>  4623
gggagaaagc uuaccaugag cgugggcauc gucgggcugc uccugaccac agccauggcu       60

gccgagguua cgagacgggg auccgcguac uauauguacc uugaccgcaa cgaugcuggc      120

gaagccauuu cuuuccccac cacacugggg augaauaagu gcuauaucca gauuauggac      180

cucggacaca ugugugaugc caccaugagc uacgagugcc cuaugcugga cgaaggcgug      240

gagccggaug acguggauug uuggugcaac acaaccucca caugggucgu guaugggacc      300

ugucaucaca aaaagggaga agcuagacgg ucucgcagag ccguuacgcu ucccagccau      360

uccacccgga aacugcaaac acgcucucag accuggcucg agagcagaga guacacaaag      420

caccugaucc ggguggaaaa uuggauuuuu cgcaacccug gcuucgcucu ggccgcggcc      480

gcuaucgccu ggcuccuggg cuccagcacc ucucagaaag ucaucuaucu ugugaugauu      540

cugcucaucg cucccgccua cuccauuaga ugcaucgggg uuagcaaucg ggacuuugug      600

gagggaaugu cuggcgggac gugggucgau guggugcugg aacauggagg cugugucaca      660

guuauggcgc aggacaagcc uaccguggau auugagcuug ugacaaccac cgucuccaac      720

auggccgagg ugcgcagcua cugcuaugaa gccucuaucu ccgacauggc uagcgauucc      780

agauguccca cacaagggga ggcuuaccug gacaagcagu cugacacgca guauguuugc      840

aaacggaccc ucguggaucg cggauggggc aauggcugug ggcuguucgg aaagggcagc      900

cuggucacau gcgccaaauu ugccugcucc aagaaaauga ccggaaagag cauucagccu      960

gaaaaccucg aguacagaau caugcugucu gugcacgggu cccaacacag cggcaugauc     1020

guuaaugaca cagggcauga aaccgaugag aaccgggcua agguggagau uacgcccaau     1080

ucucccagag cggaagccac acuuggaggc uucggcagcc uggggcucga cugugagccu     1140

cgcaccggac uggauuuuuc cgaccuuuau uaccugacca ugaacaauaa acacuggcuc     1200

guccauaagg aaugguucca cgauaucccc cugccuuggc acgcuggcgc cgacacaggg     1260

accccgcauu ggaacaauaa agaggcccug guggaauuua aggaugcuca cgccaaacgg     1320

cagacagucg ugguucucgg aucucaggag ggcgcggugc auacggcucu ggccggggcc     1380

cuugaggcug aaauggacgg cgccaaggga agacugagcu ccggccaccu caaaugccgc     1440

cugaagaugg auaagcugcg gcugaaaggg gucagcuauu cucucuguac cgcugccuuc     1500

acauuuacca agauuccggc ggagacacug cauggaaccg ugacgguuga agugcaguac     1560

gccggcaccg acgggccuug caaagucccc gcucaaaugg ccguggacau gcagacacug     1620

accccugugg gaagacucau cacagcuaac cccguuauua ccgaguccac ggaaaacagc     1680

aagaugaugc uggagcuuga ucccccuuuc ggcgacucuu acaucgucau cggggugggc     1740

gagaaaaaga uuacacacca cuggcaucgc uccggaagca ccaucggcaa ggccuuugaa     1800

gcgacaguga gaggggccaa acggauggcu guccugggag auaccgccug ggacuucggc     1860

uccguugggg gagcucucaa uucucugggc aaggggauuc accagaucuu cggcgccgcc     1920

uuuaaaagcc uguucggagg cauguccugg uuuucucaaa uucuuaucgg gacccuccug     1980

auguggcugg gacucaacac aaagaauggc agcaucuccc ugaugugucu ugcucuuggg     2040

ggagugcuca uuuuccugag cacggcggug ucugccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  4624
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 1 opt

<400>  4624
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc ccggcgcaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaaaggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaaaaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4625
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 1 opt

<400>  4625
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggaug uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuauug ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auccugcuca uugcucccgc cuacuccauc agaugcauug ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauaucgagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aauuaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguua ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacauugu caucggggug     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau ugggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucaucuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4626
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 1 opt

<400>  4626
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaugaccu ccacaugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac gcuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacaca      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc accucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacguggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugacaac caccgucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacacaagg ggaggcuuac cuggacaagc agucugacac gcaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acacagggca ugaaaccgau gagaaccggg cuaaggugga gauuacgccc     1080

aauucuccca gagcggaagc cacacuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggaccccgc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacag ucgugguucu cggaucucag gagggcgcgg ugcauacggc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagaca cugcauggaa ccgugacggu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacagcu aaccccguua uuaccgaguc cacggaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgacag ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccguug ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggagugc ucauuuuccu gagcacggcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4627
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 1 opt

<400>  4627
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accgcgaugg cccaggacaa gccuacaguu gauauugagc uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

cuugugaaug acaccgggca ugaaacggau gagaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaagc uacccuggga ggcuucggca gccucgggcu ggacugugag     1140

ccucgcaccg gacuugauuu uuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cuggcccaua aggaaugguu ccacgauauu ccccugccuu ggcacgccgg cgcugccacc     1260

gggacacccc auuggaacaa uaaagaggcg cucguggaau uuaaggacgc ucacgccaaa     1320

cggcagacgg ucgugguccu gggaucucag gagggcgccg ugcauaccgc ucuugccggg     1380

gcucuggagg ccgaaaugga uggcgcgaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggacaagcu gcggcucaaa gggguuagcu auucucugug uacagccgcu     1500

uucaccuuua caaagauccc ggccgagacc guggauggaa cggucaccgu ggaaggccag     1560

uacgggggaa cagacggccc uugcaaaguu cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ucgggagacu cauuaccgcg aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacauugu gaucggaguu     1740

ggcgagaaaa agaucaccca ucacuggcac cgcuccggga gcacaauugg aaaggccuuu     1800

gaagcuaccg uccggggcgc caaaagaaug gcugugcugg gggauaccgc cugggacuuc     1860

ggauccgugg gcggggcccu caauucucug ggcaagggaa uccaucagau uaucggcgcu     1920

gcguucaaaa gccuguuugg gggaaugucc ugguucucuc aaauucuuau cggcacacuc     1980

cugauguggc uggggcucaa cacgaagaau ggaagcaucu cccugaugug ucuugcccug     2040

ggcggggucc ucauuuuucu gagcaccgcu gugucuggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4628
<211>  2275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 1 opt

<400>  4628
gggagaaagc uuaccauggg cgccgacgcu agcaucggga uugugucccu gcuccugacc       60

acagucaugg ccgcggagau cacgagacgg ggaucugcuu acuauaugua ccuugaucgc      120

agcgacgccg gcaaggccau uuccuucguu accacacugg gggugaacaa augccacgug      180

cagaucaugg aucucggaca uaugugugac gcuaccaugu cuuaugaaug ccccaugcug      240

gaugagggcg ucgaacccga cgauguggac uguuggugca auacaaccag cacauggguu      300

guguacggga ccugucacca uaagaaagga gaggccagac ggucccgcag agcugucacg      360

cuuccgucuc acagcacccg gaagcugcaa acacgcuccc agaccuggcu cgaaagcaga      420

gaguauacaa aacaucugau uaagguggag aacuggaucu uucggaaucc cggcuucgcc      480

cugguugcgg uggccaucgc uuggcuccug ggaucuucca ccagccagaa ggucauuuac      540

cuugugauga uccugcucau ugccccugcu uauucuaucc gcugcauugg gguguccaac      600

agagauuuug ucgaaggaau gagcggcggg acguggguug acguggugcu ggagcacgga      660

ggcuguguca cagugauggc ccaggauaaa cccaccguug acaucgaacu ugugacaacc      720

accgucucua auauggcgga ggugcggucc uacugcuacg aggccagcau uuccgauaug      780

gcuucugaca gccgcugucc uacacaaggg gaagccuauc uggacaagca guccgauacg      840

caguacguuu gcaaaagaac ccucguggac cggggauggg gcaacggcug ugggcuguuc      900

ggaaagggca gccuggucac augcgcuaaa uuuaccugcu cuaagaagau gacagggaaa      960

uccauccagc ccgagaaucu cgaauaucgc aucaugcuga gcgugcacgg aucucaacau     1020

agcggcauga uugucaacga ugagaauaga gccaaggugg aaguuacccc uaacuccccg     1080

cgggccgagg cuacgcuugg gggauucggc ucucugggcc ucgacuguga gcccagaaca     1140

gggcuggauu uuagcgaccu uuacuaucug accaugaaua acaaacacug gcucgugcau     1200

aaggaauggu uccacgauau cccucugccc uggcacuccg gagcggacac cgagacaccu     1260

cauuggaaua acaaagaagc ccuggucgag uuuaaggaug cucacgccaa acgccagacc     1320

gugguugugc ucggcagcca ggaaggggcc guccauacag cucuggccgg agcgcuugag     1380

gcugagaugg acggcgccaa ggggcggcug ucuuccggcc accucaagug cagacugaaa     1440

auggauaaac uucgccugaa gggagugagc uacucucucu guacggccgc uuucaccuuu     1500

acaaaggugc ccgccgaaac ccugcauggc acaguuaccg ucgaggugca guaugcuggg     1560

cgggacggac cgugcaaagu gccugcccaa auggcggucg acaugcagac gcugaccccc     1620

gugggcagac ucauuacagc caauccuguu aucaccgaau ccacagagaa cagcaagaug     1680

augcuggaac uugauccucc cuucggggac uccuacauug ugaucggagu cggcgauaag     1740

aaaaucaccc accacuggca ucgcucuggg agcauuaucg gcaaggcuuu ugaggccacg     1800

gugcggggag cuaaaagaau ggccguucug ggcgacacag cgugggauuu cggguccgug     1860

ggaggcgucu ucaacucucu cgggaagggc auucaccaga ucuuuggcgc cgcuuucaaa     1920

agccuguuug ggggcauguc cugguucagc caaauucuga ucggaacccu ucucgugugg     1980

cugggccuga auacaaagaa cgggucuauc ucccucaccu gucuggcccu uggaggcguu     2040

augauuuuuc ugagcaccgc ugugucugcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2220

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2275


<210>  4629
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 1 opt

<400>  4629
gggagaaagc uuaccauggg cgccgacacc agcaucggga uuaucggacu gcuccugaca       60

acggcuaugg ccgcggagau uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

ucugacgccg ggaaggccau cagcuucgcu acaaccaugg gagugaacaa augccacguc      180

cagauuaugg aucugggcca uaugugugac gccacaaugu ccuaugaaug ccccaugcuc      240

gaugaggggg uugaaccuga cgauguggac uguuggugca auaccacauc uaccugggug      300

gucuacggaa cgugucacca uaagaaaggc gaggcuagac cgagccggcg cgccgugacc      360

cugcccuccc acucuacaag aaaacuucaa acccggagcc agacauggcu ggaaucccgc      420

gaguauacca agcaucucau caagguugag aacuggaucc ugagaaaucc uggcuuuacg      480

guggcggccc uggcuauggc cuggcuccug gggaacagca caucucagcg ggucauuuac      540

cuugugauga uccugcucau ugcucccgcc uauuccaucc gcugcauugg aguuagcaau      600

agagauuucg uggaaggcau gucuggggga accugggucg acguggugcu ggagcacggc      660

ggguguguca caguuauggc gcaggauaag ccuaccgugg acaucgaacu ugugaccaca      720

acggucucca acauggccga ggugcggagc uacugcuacg aggcuucuau uuccgauaug      780

gccagcgacu cccgcugucc cacccaagga gaggcuuauc uggacaaaca gucugauaca      840

caguacguuu gcaagagaac ccucguggac cggggcuggg gcaaugggug uggacuguuu      900

ggcaaaggga gccuggucac augcgccaag uuuaccugcu ccaaaaagau gacgggaaag      960

agcauccagc cugaaaaccu cgaauaucgc aucaugcugu cugugcacgg cucccaacau     1020

agcgggauga uuguuaauga uaucggacac gagacagacg aaaacagagc caaaguggag     1080

gucaccccca auucuccccg ggcugaggcg acccuuggcg gcuucgggag ccugggacuc     1140

gauugugaac cuagaacagg ccuggacuuc uccgaucuuu acuaucugac caugaacaau     1200

aagcauuggc ucgugcacaa agagugguuu cacgacauuc cccugccuug gcaugccggg     1260

gcugauacag gaacgcccca cuggaacaau aaggaagccc uggucgaguu caaagacgcc     1320

caugcuaagc gccagaccgu gguugugcuc ggcucucagg aaggggccgu ccacacagcg     1380

cuggcuggcg cccuugaggc cgagauggau ggagcuaaag gccggcuguu uagcgggcau     1440

cucaagugca gaaucaagau ggacaaacug cgccuuaagg gaguguccua cagccugugu     1500

accgccgcuu ucacauuuuc uaaaguuccg gccgaaaccc uccacggcac ggugaccguc     1560

gaggugcagu augcggggcg ggacggaccu ugcaaggugc ccgcccaaau ggcuguugau     1620

augcagacac ugaccccugu cggcagacug auuacagcca accccaucau caccgaaucc     1680

acggagaaca gccgcaugau gcucgaacug gacccgccuu ucggggauuc uuacauugug     1740

aucggcgugg gagacaaaaa gauuacacac cauuggcacc gguccggcag caccaucggg     1800

aaggcuuuug aggccacagu cagaggagcg aaacgcaugg ccgugcuugg cgauaccgcu     1860

ugggacuucg gguccguugg aggcguguuc aauucucugg ggaagggcgu ccaucaggug     1920

uuuggagccg cuuucaaaag ccucuuuggc gggauguccu gguucucuca aauucugauc     1980

ggaacccugc uuguuuggcu cggccugaac acaaagaaug ggagcaucuc ccuggccugu     2040

cucgcccugg gaggcgcuau gauuuuucuu agcacggcgg ugucugccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4630
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 1 opt

<400>  4630
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggauau uguccuugaa cacgggggau gugugacagu uauggcccaa      180

gacaaaccua cgguggauau cgagcugguc accacaaccg uguccaauau ggcggaaguu      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu gcaugggucu cagcacuccg gaaugauugu caacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guugaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu ggaguucaag gaugcucacg ccaagcggca gacagucgug      840

guucucggga gccaggaagg agcggugcau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auccccgcgg agacccuuca cgggacagug accguugaag ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauua cagccaaucc cgugaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucguuauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4631
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 1 opt

<400>  4631
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4632
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 1 opt

<400>  4632
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgg cuaugaaaca      540

gaugaggacc gggccaaggu ugaagugacc ccuaacagcc cccgcgcuga ggccacacuc      600

gggggauuug gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau      660

cuguacuacc ucacgaugaa uaacaaacau uggcuggugc acaaggaaug guuucaugac      720

auuccccuuc cuuggcacgc gggagccgac acaggcaccc cgcacuggaa uaacaaagag      780

gcucuggucg aguucaagga ugcccaugcu aagcggcaga cagugguugu gcucgggagc      840

caggaaggag ccguccacac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu      900

aaaggacgcc ucuuuuccgg ccaucugaag uguagacuua aaauggauaa gcugcggcuc      960

aaaggcgugu cuuauagccu gugcaccgcc gccuucacau uuacgaaggu ucccgcugag     1020

acccuucacg ggacagugac cgucgaagug caauacgcgg gaacagacgg cccuuguaaa     1080

auccccgucc agauggccgu ggauaugcag acccugacgc cuguugggcg ccucauuaca     1140

gcuaaucccg ugaucaccga guccaccgaa aacagcaaga ugaugcugga gcuggacccg     1200

ccuuucggag auucuuauau ugucaucggc gugggggaca agaaaaucac acaccauugg     1260

cacagauccg gcagcaccau uggaaaggcc uuugaagcca caguucgggg cgcuaaacgc     1320

auggccgugc ucggggauac ggcgugggac uucggaucuu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4633
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 1 opt

<400>  4633
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4634
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 1 opt

<400>  4634
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauaca      540

ggcuaugaaa ccgacgagaa ucgggccaag guggaaguga caccuaacag cccccgcgcu      600

gaggccaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacggggcuu      660

gacuucuccg aucuguacua ccucacaaug aauaacaaac auuggcuggu ccacaaggaa      720

ugguuucaug acauuccccu uccuuggcac gcgggagccg acaccggcac accgcacugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccaug cuaagcggca gaccguugug      840

guccucggga gccaggaagg agccgugcac accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggaaa gcucuuuucc ggccaucuga aaugucgccu uaagauggau      960

aaacugagac ucaagggcgu uucuuauagc cugugcacag ccgccuucac guuuaccaaa     1020

gugcccgcug agacacuuca cgggaccguc acaguggaag uccaauacgc gggaaccgac     1080

ggcccuugua agauccccgu gcagauggcc guugauaugc agacgcugac accugugggg     1140

cggcucauua ccgcuaaucc cgucaucacc gaguccacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuau auugugaucg gcguugggga caaaaagauc     1260

acccaccauu ggcaccgcuc cggcagcaca auuggaaaag ccuuugaagc cacggugaga     1320

ggcgcuaagc ggauggccgu ccucggggau accgcguggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4635
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 1 opt

<400>  4635
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugacagu uaacgauauc      540

ggcuaugaaa ccgacgagaa ucgggccaag guggaaguga caccuaacag cccccgcgcu      600

gaggccaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacggggcuu      660

gacuucuccg aucuguacua ccucacaaug aauaacaaac auuggcuggu ccacaaggaa      720

ugguuucaug acauuccccu uccuuggcac gcgggagccg acaccggcac accgcacugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccaug cuaagcggca gaccguugug      840

guccucggga gccaggaagg agccgugcac accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggaaa gcucuuuucc ggccaucuga aaugucgccu uaagauggau      960

aaacugagac ucaagggcgu uucuuauagc cugugcacag ccgccuucac guuuaccaaa     1020

gugcccgcug agacacuuca cgggaccguc acaguggaag uccaauacgc gggaaccgac     1080

ggcccuugua agauccccgu gcagauggcc guugauaugc agacgcugac accugugggg     1140

cggcucauua ccgcuaaucc cgucaucacc gaguccacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuau auugugaucg gcguugggga caaaaagauc     1260

acccaccauu ggcaccgcuc cggcagcaca auuggaaaag ccuuugaagc cacggugaga     1320

ggcgcuaagc ggauggccgu ccucggggau accgcguggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4636
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 1 opt

<400>  4636
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgaugaa      540

aaucgggcca agguggaggu gacaccuaac agcccccgcg cugaagccac ccugggcggg      600

uuuggaucuc ucggccugga cugcgagccu agaacaggcc uugauuucuc cgaccuguau      660

uaccucacca ugaauaacaa acauuggcug guccacaagg agugguuuca ugauauuccc      720

cuuccuuggc acgcgggggc cgacacggga acaccgcacu ggaauaacaa agaagcucug      780

guggaguuca aggacgccca ugcuaagcgg cagaccguug ugguccucgg cagccaggaa      840

ggggccgugc acacagcgcu ggccggagcu cuggaggccg aaauggaugg cgcuaaaggg      900

cgccucuuuu ccggacaucu gaaguguaga cuuaaaaugg acaagcugcg gcucaaaggc      960

guuucuuaca gccugugcac cgccgccuuc accuuuacaa aggugcccgc ugagacgcuu     1020

cacggcaccg ucacagugga gguccaauau gcggggaccg auggaccuug uaaagugccc     1080

gcccagaugg cuguugacau gcagacacug accccugugg gccgccucau cacggccaau     1140

cccgucauua cagaauccac cgagaacagc aagaugaugc uggaacugga uccgccuuuc     1200

ggggacucuu acaucgugau uggaguuggc gauaagaaaa ucacccacca uuggcacaga     1260

uccgggagca caaucggcaa ggccuuugag gcuaccgugc ggggagccaa acgcauggcg     1320

guccucggcg acacagcuug ggauuucggg ucuugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  4637
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 1 opt

<400>  4637
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgg cuaugaaaca      540

gaugagaacc gggccaaggu ugaagugacc ccuaauagcc cccgcgcuga ggccacacuc      600

gggggauuug gcucucuggg ccuggacugc gagccuagaa ccgggcuuga uuucuccgac      660

cuguacuacc ucacgaugaa caauaaacau uggcuggugc acaaggaaug guuucaugau      720

auuccccuuc cuuggcacgc gggagccgac acaggcaccc cgcacuggaa caauaaagag      780

gcucuggucg aguucaagga cgcccaugcu aagcggcaga cagugguugu gcucgggagc      840

caggaaggag ccguccacac cgcgcuggcc ggcgcucugg aagccgagau ggauggggcu      900

aaaggaaagc ucuuuuccgg ccaucugaaa ugucgccuua agauggacaa acugagacuc      960

aagggcgugu cuuauagccu gugcaccgcc gccuucacau uuacgaaagu ucccgcugag     1020

acccuucacg ggacagugac cgucgaagug caauacgcgg gaacagaugg cccuuguaag     1080

auccccgucc agauggccgu ggacaugcag acccugacgc cuguugggcg gcucauuaca     1140

gcuaaccccg ugaucaccga guccaccgaa aauagcaaga ugaugcugga gcuggauccg     1200

ccuuucggag acucuuauau ugucaucggc gugggggaua aaaagaucac acaccauugg     1260

caccgcuccg gcagcaccau uggaaaagcc uuugaagcca caguuagagg cgcuaagcgg     1320

auggccgugc ucggggacac ggcgugggau uucggaucuu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4638
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 1 opt

<400>  4638
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu cccuuagaug cauuggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuggaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau cgagcucgug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcauuucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccugga caagcagucc gauacccagu augucugcaa acggacacuu      360

guggaccgcg gguggggaaa cggcuguggc cuguuuggga agggaagccu cgugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucuggag      480

uacagaauca ugcugagcgu ccaugggucu cagcacuccg gaaugauugu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuugauu gcgagccuag aaccgggcug      660

gacuucuccg aucucuauua ccugacgaug aauaacaaac acuggcuugu gcauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccucgu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcugggga gccaggaagg agcgguccau accgcccugg cuggcgcccu cgaagcugag      900

auggacgggg ccaaaggacg ccuguccucu ggccaccuua aguguagacu gaaaauggau      960

aagcuccggc ugaaaggcgu gagcuacucc cuuugcaccg ccgcuuuuac auucacgaag     1020

auccccgcgg agacccugca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccucac gccugugggg     1140

cgccugauua cagccaaucc cguuaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcucgacc cgccuuuugg agauuccuac auugugaucg gcgucgggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucugggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4639
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 1 opt

<400>  4639
gggagaaagc uuaccaugag caagaaagug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccag      180

gacaagccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

caaggcgaag ccuaccucga caaacagucc gauacccagu augucugcaa gcggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga aaggaagccu ggugaccugc      420

gcuaaguucg ccuguucuaa gaaaaugacg ggcaagucca uccagcccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu caacacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaaa guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaagc acuggcuggu gcauaaagaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaagg aggcccuggu cgaguucaag gaugcucacg ccaaacggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaagggacg ccucuccucu ggccaccuga aauguagacu uaagauggau      960

aaacugcggc ucaagggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaaa     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaguaugc cggaacagac     1080

ggcccuugua aggugcccgc ucaaauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga gaaaaagauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaagc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4640
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 1 opt

<400>  4640
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu gguauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4641
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 1 opt

<400>  4641
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggaaa gcucuccucu ggccaccuga aaugucgccu uaagauggau      960

aaacugagac ucaagggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaaa     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aggugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cggcucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga gaaaaagauu     1260

acacaucacu ggcaucgcag cggcucuacc aucggaaagg cuuucgaagc cacagugaga     1320

ggcgcgaaac ggauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4642
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 1 opt

<400>  4642
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaccc agacgcugac accugugggg     1140

cgccucauca ccgccaaucc cguuauuacc gagagcacag aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg cuuucgaggc cacggugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac accgccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4643
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 1 opt

<400>  4643
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaaauc      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu gcaugggucu cagcacuccg gaaugauugu caacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guugaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu ggaguucaag gaugcucacg ccaagcggca gacagucgug      840

guucucggga gccaggaagg agcggugcau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auccccgcgg agacccuuca cgggacagug accguugaag ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauua cagccaaucc cgugaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucguuauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4644
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 1 opt

<400>  4644
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgcgca gaauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4645
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 1 opt

<400>  4645
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggucu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu gguuaccugc      420

gccaaauucg cuuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu gaacgauacc      540

ggccaugaaa cagacgagaa ucgggccaag guggaaauua ccccuaacag cccccgcgcu      600

gaggccacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu ccauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcgggagccg acacaggcac cccgcauugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccacg cuaagcggca gacaguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uagcuacucc cugugcaccg ccgccuuuac auucacgaag     1020

auucccgcug agacccuuca cgggacagug accgucgaag ugcaauaugc gggaacagac     1080

ggcccuugua aaguccccgc ccagauggcu guggauaugc agacccugac gccuguuggg     1140

cgccucauca cagccaaucc cgugauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg ccuucgaggc uacaguucgg     1320

ggcgccaaac gcauggcggu gcucggggac acggcuuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4646
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 1 opt

<400>  4646
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaucg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugauug gcgucgggga aaagaaaauc     1260

acccaucacu ggcauagaag cggcucuaca auuggaaagg cuuucgaggc caccgugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4647
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 1 opt

<400>  4647
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcauugggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4648
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 1 opt

<400>  4648
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cuugcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuggaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccca cggucgauau ugagcucgug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccuaca      300

cagggcgaag ccuaccugga caagcagucc gauacccagu augucugcaa acggacacuu      360

guggaccgcg gguggggaaa cggcuguggc cuguuuggga agggaagccu cgugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaaccgga gaaucuggag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua cccccaacag cccucgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuugauu gcgagcccag aaccgggcug      660

gacuucuccg aucucuauua ccugacgaug aauaacaaac acuggcuugu gcauaaggaa      720

ugguuucacg acaucccucu gcccuggcac gccggagcug acacaggcac cccucauugg      780

aauaacaaag aggcccucgu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcugggga gccaggaagg agcgguccau accgcccugg cuggcgcccu cgaagcugag      900

auggacgggg ccaaaggacg ccuguccucu ggccaccuua aguguagacu gaaaauggau      960

aagcuccggc ugaaaggcgu gagcuacucc cuuugcaccg ccgcuuuuac auucacgaag     1020

auuccggcgg agacccugca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggccccugua aagugccugc ucagauggcc gucgauaugc agacccucac gcccgugggg     1140

cgccugauca cagccaaucc uguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcucgauc ccccguuugg agacuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucugggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4649
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 1 opt

<400>  4649
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga cccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucagcucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gucuuacagc cugugcacag ccgcuuuuac guucaccaag     1020

auucccgcgg agacacuuca cgggaccguu acaguggaag uccaauaugc cggaaccgac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacgcugac accugugggg     1140

cgccucauca ccgccaaucc cguuauuacc gaguccacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauucuuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc cggcagcaca aucggaaagg cuuucgaggc cacggugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac accgccuggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4650
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 1 opt

<400>  4650
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcuggc ucagauggcc gucgauaugc agacccucac gcccgugggg     1140

cgccugauca cagccaaucc uguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcucgauc ccccguuugg agacuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucugggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4651
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 1 opt

<400>  4651
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaagcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaggcugag      900

auggaugggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggac      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg aaacccuuca cgggacaguu accguggagg uccaauaugc cggaacagau     1080

ggcccuugua aagugcccgc ucagauggcc gucgacaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gaaagcaccg agaacucuaa gaugaugcug     1200

gaacuggauc cgccuuuugg agacuccuac aucgugaucg gcgucgggga gaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggau acggccuggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4652
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 1 opt

<400>  4652
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcggccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacagggcuu      660

gacuucuccg aucuguauua ccucaccaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcuggagccg acacgggcac accgcauugg      780

aauaacaaag aggcucuggu cgaguucaag gaugcccacg cgaagcggca gaccgugguu      840

gugcucggga gccaggaagg agccguccau acagcucugg ccggcgcucu ggaagccgag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg cugcguuuac cuucacaaag     1020

auucccgccg agacgcuuca cgggaccguu acaguggaag uccaauaugc uggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauca cggcuaaucc cguuauuaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg ccuucgaggc gaccgugcgg     1320

ggcgcuaaac gcauggccgu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4653
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 1 opt

<400>  4653
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug uccaccacag uuucuaauau ggcggaagug      240

cgcagcuacu gcuaugaggc uaccaucucc gacaucgccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu ccaugggucu cagcacuccg gaaugauugu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagcggug      840

guucucggga gccaggaagg agccgugcau accgcucugg ccggcgcucu ggaagccgag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cagcuacucc cugugcaccg cugcguuuac auucacgaag     1020

auccccgccg agacccuuca cgggacagug accguugaag ugcaauaugc uggaacagac     1080

ggcccuugua aaguccccgc ccagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauua cagcuaaucc cgugaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucguuauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg ccuucgaggc gacaguccgg     1320

ggcgcuaaac gcauggccgu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4654
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 1 opt

<400>  4654
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucagcaag     1020

auucccgcgg agacgcuuca cgggaccguu acagaagagg ugcaauaugc cggaaccgac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacacugac cccugucggg     1140

cgccucauca cggccaaucc cgugauuaca gaaucuaccg agaacuccaa gaugaugcug     1200

gaacuggacc cgccuuuugg agauagcuac aucguuaucg gcguggggga gaagaaaauu     1260

acccaucacu ggcauagauc uggcagcaca aucggaaagg cuuucgaggc caccguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acagccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4655
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 1 opt

<400>  4655
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaac ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccucac gccugucggg     1140

cgccugauca cagccaaucc cgugauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcucgacc cgccuuuugg agauuccuac aucguuaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcugggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4656
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 1 opt

<400>  4656
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcauu ucuaaccggg acuucguuga ggggaugagc      120

ggaggcaccu ggguggaugu gguccuugaa cacgggggau gugugacagu uauggcccaa      180

gacaaaccua cgguggauau cgagcugguc accacaaccg uguccaauau ggcggaaguu      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu gcaugggucu cagcacuccg gaaugauugu caacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guugaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcauucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucucug aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu ggaguucaag gaugcucacg ccaagcggca gacagucgug      840

guucucgggu cccaggaagg agcggugcau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucagcucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cucuuacagc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacagug accguugaag ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauca cagccaaucc cgugaucacc gaguccaccg aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauucuuac auuguuaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagauc cggcagcacc aucggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4657
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 1 opt

<400>  4657
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ugaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uagcuacucc cugugcaccg ccgccuuuac auucacgaag     1020

auucccgcug agacccuuca cgggacagug accgucgaag ugcaauaugc gggaacagac     1080

ggcccuugua aaguccccgc ccagauggcu guggauaugc agacccugac gccuguuggg     1140

cgccucauca cagccaaucc cgugauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg ccuucgaggc uacaguucgg     1320

ggcgccaaac gcauggcggu gcucggggac acggcuuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4658
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 1 opt

<400>  4658
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggagcugac     1080

ggcccuugua aagugcccgc ccagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauca cggcuaaucc cguuauuaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg ccuucgaggc gaccgugcgg     1320

ggcgcuaaac gcauggccgu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4659
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 1 opt

<400>  4659
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac acaggagccg acaccggcac accgcauugg      780

aauaacaaag aggcucuggu cgaguucaag gaugcccacg cuaagcggca gaccgugguu      840

gugcucggga gccaggaagg agccguccau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcacag ccgccuuuac guucaccaag     1020

auucccgcug agacacuuca cgggaccguu acaguggaag uccaauaugc gggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcu gucgauaugc agacgcugac accugugggg     1140

cgccucauca ccgccaaucc cguuauuacc gagggaacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg cgauucuuac aucgugaucg gggucggcga aaagaaaauu     1260

acccaucacu ggcauagauc cggaagcaca aucggcaagg ccuucgaggc uacggugcgc     1320

ggggccaaac ggauggcggu ucucggagac accgcuuggg auuuuggcuc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4660
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 1 opt

<400>  4660
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagc cauggcgcaa      180

gacaaaccua cgguggauau ugagcugguc accacaaccg uguccaauau ggcugaaguu      240

cgcucuuacu gcuaugaggc cagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgagg cuuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gccaaauucg cuuguucuaa gaagaugacg ggcaaaucca uccaacccga aaaucucgag      480

uacagaauua ugcugagcgu gcaugggucu cagcacuccg gaaugcuggu caacgauacc      540

ggccaugaaa cagacgagaa ucgggccaag guugaaauca ccccuaacag cccccgcgcg      600

gaggccacac ucgggggauu uggcucucug ggccuugauu gcgagccuag aaccgggcug      660

gacuucuccg aucucuauua ccugacgaug aauaacaaac acuggcuugc ucauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gccggagcug ccacaggcac cccgcauugg      780

aauaacaaag aggcgcucgu ggaguucaag gacgcccacg cuaagcggca gacagugguc      840

gugcugggga gccaggaagg agccguucau accgcucugg ccggcgcccu cgaagcugag      900

auggaugggg cgaaaggacg ccuguccucu ggccaccuua aguguagacu gaaaauggac      960

aagcuccggc ugaaaggcgu gagcuacucc cuuugcaccg ccgcuuuuac auucacgaag     1020

auccccgccg agaccgucga ugggacagug accguugaag gacaauaugg cgggacagac     1080

ggaccuugua aagugcccgc ccagauggcu gucgauaugc agacccugac gccugugggc     1140

cgccucauua cagccaaucc cgucaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg ggauuccuac auugugaucg gcguuggaga aaagaaaauc     1260

acacaccauu ggcacagaag cggcucuacc auugggaagg cguucgaggc uacagugcgg     1320

ggagccaaac gcauggccgu ccucggcgac acggcuuggg auuuugggag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4661
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 1 opt

<400>  4661
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccug cggucgauau ugagcuggug acgaccacag uuuccaauau ggcugaagug      240

cgcucuuacu gcuaugaggc cagcaucucc gacauggccu cugauagcag auguccgacc      300

cagggcgagg cuuaccucga caagcagucc gauacacagu augucugcaa acggacccug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugacaugc      420

gccaaauucg cuuguucuaa gaagaugacc ggcaaaucca uccaacccga aaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacg      540

ggccaugaaa ccgacgagaa ucgggccaag guggaaauua caccuaacag cccccgcgcg      600

gaggccaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacagggcuu      660

gacuucuccg aucuguauua ccucaccaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcuggagccg acacgggcac accgcauugg      780

aauaacaaag aggcucuggu cgaguucaag gaugcccacg cgaagcggca gaccgugguu      840

gugcucggga gccaggaagg agccguccau acagcucugg ccggcgcucu ggaagccgag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg cugcguuuac cuucacaaag     1020

auucccgccg agacgcuuca cgggaccguu acaguggaag uccaauaugc uggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauca cggcuaaucc cguuauuaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg ccuucgaggc gaccgugcgg     1320

ggcgcuaaac gcauggccgu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4662
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 1 opt

<400>  4662
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu aacaaucggg acuucgugga ggggaugucu      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuagcaacau ggcggaagug      240

cgcuccuacu gcuaugaggc uucuaucagc gacauggccu ccgauucuag auguccgaca      300

cagggcgaag ccuaccucga caagcagagc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa uggcuguggc cuuuuuggga agggaucccu ggugaccugc      420

gcuaaauucg ccuguagcaa gaagaugacg ggcaaaucua uccaacccga gaaccucgag      480

uacagaauua ugcuguccgu ccaugggagc cagcacucug gaaugaucgu uaaugauacc      540

ggccaugaaa cagacgagaa ccgggcuaag guggaaauua ccccuaauag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucccug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucucug aucuguauua ccucacgaug aacaauaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aacaauaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucgggu cccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucagcucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gucuuacagc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaccc cguuauuacc gaguccaccg aaaauagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauucuuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc cggcagcacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4663
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 1 opt

<400>  4663
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcgccg cugcguuuac cuucacaaag     1020

auucccgccg agacgcuuca cgggaccguu acaguggaag uccaauaugc uggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauca cggcuaaucc cguuauuaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg ccuucgaggc gaccgugcgg     1320

ggcgcuaaac gcauggccgu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4664
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 1 opt

<400>  4664
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauauu      540

ggccaugaaa ccgacgagaa ucgggcuaag guggaaauca caccuaacag cccccgcgcc      600

gaggcgaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacagggcuu      660

gacuucuccg aucuguauua ccucaccaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacgggcac accgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gaccgugguu      840

gugcucggga gccaggaagg agcgguccau acagcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac cuucacaaag     1020

auccccgcgg agacgcuuca cgggaccguu acaguggaag uccaauaugc cggaaccgac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauua cggccaaucc cguuaucaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugauug gcgucgggga caagaaaauc     1260

acccaucacu ggcauagaag cggcucuaca auuggaaagg cuuucgaagc caccgugcgg     1320

ggcgcgaaac gcauggcugu ucucggggau acagccuggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4665
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 1 opt

<400>  4665
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga caagaaaauu     1260

acacaucacu gguauagaag cggcucuacc aucggaaagg cuuucgaagc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggau acggccuggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4666
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 1 opt

<400>  4666
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauaca      540

ggccaugaaa ccgacgagaa ucgggccaag guggaaguga caccuaacag cccccgcgcu      600

gaggccaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacggggcuu      660

gacuucuccg aucuguauua ccucacaaug aauaacaaac acuggcuggu ccauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gcgggagccg acaccggcac accgcauugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccacg cuaagcggca gaccguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuuuucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uucuuacagc cugugcacag ccgccuucac guuuaccaag     1020

gugcccgcug agacacuuca cgggaccguc acaguggaag uccaauaugc gggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcu guugauaugc agacgcugac accugugggg     1140

cgccucauca ccgccaaucc cgucauuacc gaguccacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuac aucgugauug gcguugggga caagaaaauc     1260

acccaucacu ggcauagauc cggcagcaca aucggaaagg ccuuugaagc uacggugcgg     1320

ggcgccaaac gcauggcggu ccucggggau accgcuuggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4667
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 1 opt

<400>  4667
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauauu      540

ggccaugaaa cagacgagaa ucgggccaag guggaaguga ccccuaacag cccccgcgcu      600

gaggccacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu ccauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcgggagccg acacaggcac cccgcauugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccacg cuaagcggca gacaguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuuuucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uucuuacagc cugugcaccg ccgccuucac auuuacgaag     1020

gugcccgcug agacccuuca cgggacaguc accguggaag uccaauaugc gggaacagac     1080

ggcccuugua aagugcccgc ccagauggcu guugauaugc agacccugac gccugugggg     1140

cgccucauua cagccaaucc cgucaucacc gaguccaccg aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuac auugugaucg gcguugggga caagaaaauc     1260

acacaucacu ggcauagauc cggcagcacc auuggaaagg ccuuugaagc uacagugcgg     1320

ggcgccaaac gcauggcggu ccucggggau acggcuuggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4668
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 1 opt

<400>  4668
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauauu      540

ggccaugaaa cagacgagaa ucgggccaag guggaaguga ccccuaacag cccccgcgcu      600

gaggccacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu ccauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcgggagccg acacaggcac cccgcauugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccacg cuaagcggca gacaguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuuuucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uucuuacagc cugugcaccg ccguguguac agccgcuaag     1020

guccccgcgg agacgcuuca cgggaccgug acagucgaag ugcaauaugc cggaaccgac     1080

ggcccuugca aaguucccgc ucagauggcc guggauaugc agacacugac cccugucggg     1140

cgccucauua cggccaaucc cgugaucaca gaguccaccg aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuac auuguuaucg gcguggggga caagaaaauc     1260

acccaucacu ggcauagauc cggcagcaca auuggaaagg cuuuugaagc caccguccgg     1320

ggcgcgaaac gcauggcugu gcucggggau acagccuggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4669
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 1 opt

<400>  4669
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgaugaa      540

aaucgggcca agguggaggu gacaccuaac agcccccgcg cugaagccac ccugggcggg      600

uuuggaucuc ucggccugga cugcgagccu agaacaggcc uugauuucuc cgaccuguau      660

uaccucacca ugaauaacaa acauuggcug guccacaagg agugguuuca ugauauuccc      720

cuuccuuggc acagcggggc ggacacggaa acaccgcacu ggaauaacaa agaggcccug      780

guggaguuca aggacgcuca ugccaagcgg cagaccguug ugguccucgg aucccaggaa      840

ggcgcugugc acacagcccu ggcgggggcc cuggaagcug agauggaugg agccaaaggc      900

cgccucucua gcgggcaucu gaaguguaga cuuaaaaugg acaagcugcg gcucaaagga      960

guuuccuaca gccugugcac cgcugccuuu accuucacaa aggugcccgc cgagacgcuu     1020

cacggcaccg ucacagugga aguccaauau gcuggccgcg augggccuug uaaagugccc     1080

gcgcagaugg ccguugacau gcagacccug acaccugugg gaagacucau caccgcuaau     1140

cccgucauua cggagucuac agaaaacucc aagaugaugc uggagcugga uccgccuuuu     1200

ggcgacagcu acaucgugau ugggguugga gauaagaaaa ucacccacca uuggcaccgg     1260

ucuggcagca ucauugggaa ggccuucgaa gccaccgugc gcggcgcuaa aagaauggcc     1320

guccucggag acacagcgug ggauuuuggc uccugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  4670
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 1 opt

<400>  4670
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauugu ucucaucgug       60

cccgccuauu ccauuucuug caucggcgug agcaacagag accuggucga ggggaugucc      120

ggaggcaccu ggguggaugu ugugcuugaa cacgggggau gugucacaga gauggcucaa      180

gacaaaccua cgguggauau ugaacugguu accaugacag ugucuaauau ggccgagguc      240

cggagcuacu gcuaugaagc gucccucucu gacauggcua gcgccucccg cuguccgacc      300

cagggcgagc ccagccugga uaagcagucu gacacacagu ccgugugcaa aagaacccuu      360

ggggaucggg gauggggcaa cggcuguggg aucuucggaa agggcagccu ggugacaugc      420

ucuaaauuua ccuguugcaa gaagaugccu gggaaaucca uccaacccga gaaucucgaa      480

uaccgcauua ugcugccugu ccauggaagc cagcacucug gcaugaucgu uaacgacauu      540

gggcaugaga cggaugaaaa uagagccaag guggagguga cccccaacuc cccucgggcu      600

gaggccacac ugggaggcuu cggcagccuc gggcuggacu gugaaccgcg caccggacuu      660

gauuuuuccg accuguauua ccucacaaug aauaacaaac acuggcuggu ccauaaggag      720

ugguuccacg auaucccccu uccuuggcac gcuggcgccg acaccgggac gccccauugg      780

aauaacaaag aagcgcuggu ggaguuuaag gacgcccacg cuaagagaca gacaguugug      840

guccucggau cucaggaagg cgccgugcau accgcucugg ccggggcgcu ggaggccgag      900

auggauggag cuaaaggccg ccucuucagc ggccaccuga agugccggcu uaaaauggac      960

aagcugagac ucaaaggggu uuccuauagc cuguguacag ccgcuuuuac cuucaccaag     1020

gugccugccg aaacacuuca cggaacgguc accguggagg uccaaucugc cggcacagau     1080

gggcccugca aagugccggc ucagauggcg guugacaugc agacccugac accuguggga     1140

cggcucauua ccgccaaucc cgucaucacg gaauccacag agaacagcaa gaugaugcug     1200

gaacuggauc cucccuuugg cgacucuuac auugugaucg ggguuggcga uaagaaaauc     1260

acccaucacu ggcaucgcag cggauccacc auuggcaagg cuuucgaggc cacagugaga     1320

ggggccaaac ggauggcugu ccucggagac accgccuggg auuuuggcuc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4671
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 1 opt

<400>  4671
gggagaaagc uuaccaugag acagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuaua gcauucggug caucggcguu uccaaccgcg acuucgugga ggggaugucu      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuagcaauau ggcggaagug      240

agauccuacu gcuaugaggc uucuaucagc gacauggccu ccgauucucg guguccgaca      300

cagggcgaag ccuaccucga caagcagagc gauacccagu augucugcaa acgcacacug      360

guggacagag gguggggaaa cggcuguggc cuuuuuggga agggaucccu ggugaccugc      420

gcuaaauucg ccuguagcaa gaagaugacg ggcaaaucua uccaacccga gaaucucgag      480

uaccggauua ugcuguccgu ccaugggagc cagcacucug gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgcgcuaag guggaaauua ccccuaacuc ccccagagcc      600

gaggcgacac ucgggggauu uggcagccug ggccuggauu gcgagccucg gaccgggcuu      660

gacuucucug aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcgcca gacagugguu      840

gugcucgggu cccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggaag acucagcucc ggccaccuga agugucggcu uaaaauggau      960

aagcugcgcc ucaaaggcgu gucuuacagc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

agacucauca cagccaaucc cguuauuacc gaguccaccg aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauucuuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcaucgguc cggcagcacc aucggaaagg cuuucgaggc cacagugcgc     1320

ggcgcgaaaa gaauggcugu ucucggggac acggccuggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4672
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 1 opt

<400>  4672
gggagaaagc uuaccaugag ccagagagug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauucggug caucggcguu ucuaaccgcg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaagccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

agaucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagccg guguccgaca      300

cagggcgaag ccuaccucga caaacagucc gauacccagu augucugcaa gcgcacacug      360

guggacagag gguggggaaa cggcuguggc cuuuuuggga aaggaagccu ggugaccugc      420

gcuaaguuca cguguucuaa aaagaugacc ggcaagucca uccaacccga gaaucucgag      480

uaccggauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauauu      540

ggccaugaaa cagacgagaa ucgcgccaaa guggaaguga ccccuaacag ccccagagcu      600

gaggccacac ucgggggauu uggcucucug ggccuggauu gcgagccucg gaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaagc acuggcuggu ccauaaagaa      720

ugguuucacg acaucccccu uccuuggcac gcgggagccg acacaggcac cccgcauugg      780

aauaacaagg aggcucuggu ggaguucaaa gaugcccacg cuaagcgcca gacaguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaagggaag acucuuuucc ggccaccuga aaugucggau uaagauggau      960

aaacuucgcc ugaagggcgu uucuuacagc cucugcaccg ccgccuucac auuuuccaaa     1020

gugcccgcug agacgcugca cgggaccguc acaguggaag uccaauaugc gggaagagac     1080

ggcccuugua aggugcccgc ccagauggcu guugauaugc agacccuuac accugugggg     1140

cggcugauca ccgccaaucc cauuaucacg gagagcacag aaaacucucg caugaugcuc     1200

gagcuggacc cgccuuucgg agauuccuac aucgucauug gcguggggga caaaaagauc     1260

acccaucacu ggcauagaag cggcucuacc auuggaaagg ccuuugaagc uacaguucgg     1320

ggcgccaaaa gaauggcggu gcugggggau accgcuuggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4673
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  4673
gggagaaagc uuaccaugag gauaauaaau gccagaaagg aaaaaaaaag aagaggagcu       60

gauacaagug uaggaauagu aggauuauua uugacaacag caauggcagc ugaaguaacg      120

agaagagguu cagcauauua uauguacuua gacaggaaug augcagguga agcaauauca      180

uucccaacaa cacuaggaau gaauaaaugu uauauacaaa uuauggaccu cggacauaug      240

ugugaugcua cgaugucaua ugaaugcccu augcuggacg agggaguuga accagaugau      300

guugauuguu ggugcaacac aacaaguaca ugggucguau auggaacaug ucaucauaaa      360

aaaggagaag cacgaaggag uagaagagcu guaacauuac caucacauuc aacgcgaaag      420

cuacaaacgc gaucacaaac augguuagaa agcagagaau acaccaaaca ucuaauuaga      480

guagagaauu ggauuuuuag aaauccagga uuugcacugg cggccgcugc aauugcaugg      540

cuauuaggaa guucaaccag ucaaaaagua auauaccuag uaaugauauu acuaauagcg      600

ccagcuuaua gcauaagaug cauaggaguu aguaaccgag auuuugucga gggaauguca      660

ggugguacuu ggguagacau agugcuagaa caugguggau guguaacagu uauggcacag      720

gacaaaccaa caguagacau ugaacucgua acaacaacug uaaguaauau ggcagaagua      780

cggucauacu guuaugaagc uaguauauca gauauggcaa gcgacagcag augcccaacu      840

caaggggaag cauacuuaga uaaacaaagu gauacucagu auguauguaa acgaacauua      900

guagacagag gauggggaaa cggaugugga cuuuucggua aagguagccu agugacaugu      960

gcaaaauuug cuuguaguaa aaaaaugacu ggaaaaucaa uacaacccga aaaucuagaa     1020

uauagaauaa uguugucagu ccacggauca cagcauucug guaugauugu aaaugauacu     1080

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccaaacag uccaagggca     1140

gaagcaacau uaggugguuu uggaucacua ggguuagauu gugaaccaag gacgggauua     1200

gauuuuagcg acuuauacua uuuaaccaug aacaauaaac acugguuagu acacaaagaa     1260

ugguuucacg auauaccguu gccguggcau gcgggagcug auacaggcac accacauugg     1320

aauaauaaag aagcccuagu agaauucaaa gacgcacacg cgaaaagaca aacaguagua     1380

guauuagguu cgcaagaagg cgcagugcau accgcauugg caggugcauu ggaagcagaa     1440

auggauggag caaaaggaag guuaaguucu ggacauuuga aauguagauu gaaaauggau     1500

aaacuaagac uaaaaggagu gagcuauuca uuauguaccg cagcauuuac auucacaaaa     1560

auuccagcag aaacguuaca cggaacaguu acgguagaag uacaauaugc agggacagau     1620

ggccccugua aaguaccagc ucaaauggcg guugauaugc aaacccuaac cccaguggga     1680

agauuaauua cagcuaaccc aguaauaaca gaaaguaccg aaaauagcaa aaugaugcuu     1740

gaacuagacc caccauuugg agauucauac auaguaauag gaguuggaga aaaaaagaua     1800

acacaucauu ggcauaggag uggaaguacu aucggaaaag cguuugaagc gacaguuaga     1860

ggagcaaaaa gaauggcugu guuaggggau acagcauggg auuuuggaag cgucggagga     1920

gccuuaaauu cuuuaggaaa aggaauucau caaauauuug gggccgcauu caaaagucua     1980

uuugguggaa ugucaugguu uucacaaaua uuaauaggaa cauuguuaau guggcuagga     2040

uuaaacacua aaaacggaag cauaucacua augugucuag cuuugggugg aguacuaaua     2100

uuucuaucca cugcuguauc agcagacgug ggguguagcg uggacuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4674
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 opt

<400>  4674
gggagaaagc uuaccaugag aauaaucaau gcgaggaaag aaaagaaaag aagaggagcc       60

gacacuagcg ucgguauagu agguuuauua uuaacaacug ccauggccgc cgaaguuaca      120

agaagagguu cagcauacua cauguauuua gauagaaaug augcaggaga agcuauuucc      180

uuuccuacaa cucuuggaau gaauaaaugu uauauccaaa uuauggaucu aggacauacg      240

ugcgaugcaa caaugagcua cgaaugucca auguuagaug aaggaguaga accagaugac      300

guugauugcu gguguaacac aacuucaaca uggguaguuu auggcacaug ucaucauaag      360

aaaggagaag caagaagaag uagacgugca guaacauuac caagucauuc aacuagaaaa      420

cuacaaacua ggagccaaac auggcuugaa aguagagagu auacaaaaca ccuuauuaga      480

guagaaaauu ggauauuuag gaauccagga uucgcguuag cagcagcugc aauagcaugg      540

uugcuaggaa gcucaacaag ucaaaaagua auauauuuag uaaugauacu ucuaauagca      600

ccagcauauu caauaagaug uaucggagua ucaaaccgag auuuugucga aggaaugucu      660

ggugguacau ggguagacgu aguauuagag cacggaggau guguaacagu aauggcacaa      720

gauaaaccaa caguggauau agaauugguc acaacaaccg uaucaaauau ggcugaaguc      780

agaagcuacu gcuaugaagc aucgauaagu gauauggcga gugacucuag augcccaacc      840

caaggagaag cauacuuaga uaaacaauca gacacacaau acguauguaa aagaacuuua      900

guagauagag guuggggcaa cggaugugga cucuuuggaa aaggaucgcu aguuacaugu      960

gcaaaauuug ccuguucaaa gaaaaugacg ggaaaaucaa uacagccaga aaauuuagaa     1020

uauagaauaa uguugagugu acauggaucc caacauucag gaaugauagu aaaugauaca     1080

ggacaugaaa cugacgaaaa uagagcaaaa guugaaauaa caccaaauuc accacgugca     1140

gaggcgacac ucggugguuu cgggucauug ggucuugauu gugaaccaag aacaggauua     1200

gauuuuucag auuuauauua cuuaacaaug aacaauaaac acugguuagu acauaaagaa     1260

ugguuucaug auauaccguu accauggcau gcaggagcag auacaggcac accacauugg     1320

aauaauaaag aagcauuagu ugaguucaaa gaugcgcacg cuaaaagaca aacaguuguc     1380

guauuaggua gucaagaagg agcaguacau acagcgcuag cuggagcacu agaagcugaa     1440

auggauggag caaaaggaag acuaaguagu ggucauuuaa aauguagauu aaaaauggac     1500

aaacuuaggu uaaaaggagu uaguuacuca uuauguacug cggcuuuuac auuuacaaaa     1560

auaccugcag aaacauuaca cgguacaguu acagucgaag uacaauaugc ugguacagac     1620

ggaccuugua aaguaccagc ucaaauggca guagauaugc aaacacuaac accaguugga     1680

cgccuaauaa ccgcaaaucc uguuauaaca gaaucuacgg aaaacucaaa aaugauguua     1740

gaauuagauc ccccauuugg ggauucauau auaguaauug ggguaggaga aaagaaaaua     1800

acacaucacu ggcacagaag uggaaguaca auaggaaaag cauucgaagc aacaguaaga     1860

ggagcaaaaa gaauggcagu ccuaggggac acugcauggg auuucggaag cguaggaggg     1920

gcuuuaaaua gucucgggaa aggaauccau caaaucuuug gagcagcguu uaaaucacua     1980

uuuggaggaa ugucaugguu uucacaaaua uuaauaggua cuuuacuaau gugguuagga     2040

cuaaaugcua aaaacggcuc aauuucauua auguguuuag cauuaggagg aguuuugaua     2100

uucuuaucaa cagcaguuag cgcugacguu ggauguagug uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4675
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 opt

<400>  4675
gggagaaagc uuaccaugcg aauaauaaac gcgagaaaag aacguaaacg ucgaggagca       60

gacacaagca uaggcauaau aggauuacug cuaacaaccg caauggcagc cgaaauaaca      120

agaagaggau cagccuauua uauguaucua gauagaagug augcagguaa agccauauca      180

uuugcaacca cguuaggagu aaauaaaugc cauguacaaa uaauggaccu uggacauaug      240

ugcgaugcaa caaugucuua ugaauguccu augcuagaug aaggaguaga accagaugac      300

guugauuguu gguguaauac aacuuccaca uggguagucu acggaacaug ccaucacaaa      360

aaaggugaag cuaggagguc aagacgagcc guaacauuac caucucauuc aacaagaaaa      420

uuacaaacaa gaucacaaac auggcuagaa ucaagggaau auacaaaaca ucuuauaaaa      480

guagaaaauu ggauuuucag aaauccuggu uuugcacuag uagcaguagc aauagccugg      540

cucuuaggua gcaguaccag ccaaaaagua auauaucucg ucaugauucu auuaauagcu      600

ccagccuaca gcauaagaug uauuggagua ucaaauagag auuuuguaga agguaugucu      660

ggaggaacau ggguugaugu aguuuuagaa caugggggau guguaacagu aauggcacaa      720

gauaaaccaa caguggacau ugaauuaguc accacaacug uaucuaauau ggcagaggua      780

cgaucauauu guuaugaagc aagcauaagc gauauggcga gugauucaag augccccaca      840

caaggagaag cuuauuuaga uaaacaaagu gauacacagu auguaugcaa aagaacccua      900

guggauagag guugggguaa ugguugcgga uuauuuggaa aaggaagucu aguaacaugu      960

gcaaaauuca cuuguucaaa aaaaaugaca gggaaaagua uacaaccaga aaauuuagaa     1020

uauagaauaa uguuaagcgu acaugguuca caacauucgg gaaugauagg auaugaaaca     1080

gaugaagaua gggccaaagu agaaguaaca ccaaacaguc caagagcaga agccacacuu     1140

ggugguuucg gaucucucgg auuagacugu gaaccucgaa cagggcuuga cuuuucagau     1200

cuauacuauu uaacaaugaa uaauaagcau uggcuaguuc acaaagaaug guuucacgau     1260

auuccgcuuc cauggcaugc gggagcagau acaggaacuc cacauuggaa uaauaaagaa     1320

gcacuaguug aauucaaaga ugcacaugca aaaagacaaa cagucguagu uuuaggauca     1380

caagaaggag caguacauac agcacuagcc ggagcuuuag aagcagaaau ggauggugca     1440

aaaggacgac uauuuagcgg acaccuaaaa ugucgucuaa aaauggauaa acuuagauua     1500

aaaggaguau cguacucguu auguacagca gcauuuacau uuacuaaagu gcccgcagaa     1560

acacuacaug gaacagugac uguugaagua caauaugcag gaacagaugg cccauguaaa     1620

auuccuguuc aaauggcagu ugauaugcaa acauuaacuc caguuggaag acuaauaaca     1680

gcaaacccug uuauaacaga aucaacugaa aauaguaaaa ugauguugga acuggaccca     1740

ccauucggag auaguuacau aguaauaggu guaggagaua agaaaauaac acaucauugg     1800

cacagaucag guaguaccau ugguaaagca uucgaagcaa caguuagagg agcaaaaaga     1860

auggcaguuc ucggagauac agcaugggau uuugguagug ugggaggugu uuuuaacagu     1920

uuagguaaag gaauacauca aaucuuuggu gcagcuuuua aaagccuauu uggaggaaug     1980

ucaugguucu cucaaauucu aauagguaca cuauuaguuu ggcuaggacu uaauacgaaa     2040

aaugggucua uuaguuuaac uuguuuagca cucggaggag uaaugauauu cuuaaguaca     2100

gcaguauccg cugacgucgg uuguucagua gauuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4676
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 1 opt

<400>  4676
gggagaaagc uuaccaugag aauaauaaau gcaaggaaag aaaagaaaag aagaggagca       60

gacacaagcg uagguauagu aggguuauua uuaacaacug cuauggcugc ugaaguaaca      120

aggaggggua gugcauauua uauguauuua gacagaaacg augcagggga agcaauaagu      180

uuuccuacaa cauuaggaau gaauaaaugu uauauccaaa uuauggacuu aggccauaug      240

ugugacgcua caaugaguua cgaaugucca auguuagaug aagguguaga accagacgau      300

guggauuguu gguguaacac aacaucaaca uggguaguau augguacaug ucaucauaag      360

aaaggagaag caagaagauc aaggagagcu guaacucuac cuucacacag cacaagaaaa      420

uuacaaacua gaucacagac cugguuagaa ucuagagaau auacaaaaca uuuaaucaga      480

guagaaaauu ggaucuucag aaaccccggg uuugcucucg cagcagcagc aauagcaugg      540

uuacuuggaa guucuacauc acaaaaaguu auuuaccuag uaaugauucu acuaauagca      600

ccagcuuaua gcauaagaug cauuggaguu aguaacagag acuuuguaga aggaauguca      660

ggugguacuu gggucgaugu gguauuagaa caugggggau gcguaacugu uauggcacaa      720

gacaaaccaa caguagauau cgagcuagua acaacaacgg uaagcaacau ggcagaagug      780

agaaguuauu guuaugaagc aagcauaagu gauauggcau ccgauucacg auguccaaca      840

caaggugaag cauaccuaga uaaacaauca gauacacaau auguauguaa aagaacauua      900

guugauaggg guuggggaaa uggaugugga cuauucggaa aagggucauu agugacgugu      960

gcaaaauuug ccugcaguaa gaaaaugacu gggaaaagca uucaaccaga aaaucuagaa     1020

uauagaauaa ugcuaagugu acauggaucu caacauagug gcaugauagu aaacgauaca     1080

ggacacgaaa cugacgaaaa cagagcuaaa guagaaauca cucccaauag cccaagagcu     1140

gaagcaacac uaggaggauu uggaucauua ggacuugacu gugaaccuag aacaggauug     1200

gacuuuagcg aucuauacua ucuaacaaug aauaauaaac auuggcuagu acauaaagaa     1260

ugguuucaug auauuccacu uccauggcac gccggcgcag acacagggac cccucacugg     1320

aacaauaaag aagcauuagu agaguucaaa gaugcucaug caaaaagaca aacaguaguu     1380

guauuagguu cgcaagaagg agccguacau acugcacuug caggagcuuu agaagcagaa     1440

auggauggag caaaaggacg auuaucuagu ggacaucuaa aauguagacu aaaaauggac     1500

aaauuaagau uaaaaggagu aagcuauuca uuauguacag cagcauuuac auucacaaaa     1560

auaccugcug aaacccuaca cggcacagua acaguagaag uucaauaugc aggaacugau     1620

ggaccuugca aaguacccgc acaaauggca guugauaugc aaaccuuaac cccuguuggc     1680

agacuaauca cagcaaaccc aguuauaaca gaaucaacag aaaauagcaa aaugaugcua     1740

gaacuggauc caccuuucgg ugauucauau auaguaauag gaguagggga aaagaaaauu     1800

acucaucacu ggcauagauc aggaucaaca aucgguaaag cauucgaagc uacaguacga     1860

ggggcaaaaa gaauggcagu acuaggagau acugcauggg auuuugguag uguaggugga     1920

gcacuaaauu ccuugggaaa aggaauacac caaauauuug gagcugcauu uaaaagucua     1980

uuuggaggaa ugagcugguu uagccagauu cuaauuggaa cauuacuuau guggcucgga     2040

uuaaacacaa agaauggaag uauuucauua auguguuuag cucucggugg cguauuaaua     2100

uuuuuaagua cagcaguuag cgcagacguu ggauguucag uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4677
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  4677
gggagaaagc uuaccaugac aagcguagga auuguuggac uauugcuaac aacugcaaug       60

gcugcagagg uaacucgaag aggcagcgca uacuacaugu auuuggauag aaaugacgca      120

ggggaagcaa uaagcuuucc aacgacauua ggaaugaaua aauguuauau acaaauaaug      180

gauuuaggac acauguguga ugcaacuaug aguuaugagu gcccaaugcu ugaugaagga      240

gucgaaccag augauguuga uuguuggugu aauaccacaa guacaugggu ugucuacgga      300

acaugucacc auaaaaaagg ugaagcuaga agaaguagaa gagcaguaac acuacccagu      360

cacucaacua gaaaacuaca aaccagaucc caaacauggu uagaaaguag ggaauacaca      420

aaacaucuaa uuagaguuga aaacuggaua uuuagaaauc cagguuucgc uuuagcagca      480

gcagcaauug cuugguuauu aggaaguuca acaucacaga aaguuauaua ccuaguaaug      540

auacuacuaa uugcacccgc auauaguaua cgauguauug gaguuagcaa ucgugauuuc      600

guugaaggaa ugucaggagg aacuuggguu gacauaguau uagaacaugg agggugugua      660

acgguuaugg cacaagauaa accaacgguu gauauagagc uaguaacaac aacaguuagc      720

aauauggcag aaguacgauc auauugcuau gaagcuucaa uaucagauau ggcaucagau      780

ucgagauguc caacacaagg ggaagcauau uuagauaaac aaagugauac acaauaugua      840

uguaaacgaa cacuaguaga uagagguugg gggaauggau gugguuuauu cggaaaagga      900

ucacuuguaa caugugcaaa guuugcaugu ucaaaaaaaa ugacaggcaa aagcauacaa      960

ccagaaaauc uagaauacag aaucauguua ucaguacaug gaucucaaca uagcggaaug     1020

auaguuaaug acacaggaca ugaaacagau gaaaauagag cgaaaguaga gauaacaccu     1080

aauaguccaa gagcagaagc aacauuggga ggauucggau cucuaggacu agauugugaa     1140

ccacgaacag gacuugacuu uucugauuua uacuaucuaa caaugaacaa uaaacacugg     1200

cuaguacaua aagaaugguu ucacgauaua ccacuaccau ggcacgccgg agcagauacc     1260

ggaacaccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc ccaugcuaaa     1320

agacaaacag uuguagugcu uggaagucaa gaaggagcag uacauacagc auuggcuggu     1380

gcauuagaag cagaaaugga uggugcaaaa ggaaggcuaa guucuggaca ucuaaaaugu     1440

aggcuaaaaa uggauaaauu aagauuaaaa ggaguaucau auucauuaug uaccgcugcc     1500

uucacuuuua caaaaauacc agcagaaacg cugcacggaa cuguaacagu ugaaguacaa     1560

uaugcuggaa cagauggacc augcaaaguu ccugcacaaa uggcuguaga caugcaaaca     1620

cugacuccug uugggagacu aauuacagca aaccccguaa uaacagaaag uaccgaaaac     1680

ucuaaaauga uguuagaauu agauccaccu uucggggaua guuauauagu aauaggagua     1740

ggggaaaaaa aaauaacuca ucacuggcau aggagcggca guacuauagg uaaagccuuu     1800

gaagcaacug ucagaggugc aaaaagaaug gcaguguuag gugacacagc augggauuuc     1860

ggaucaguag gaggugcauu aaauaguuua ggcaaaggca uucaucaaau auuuggggcc     1920

gcauucaaaa gucuguucgg aggaaugagc ugguucaguc agauauuaau aggaacacua     1980

uuaauguggc uaggucuaaa cacaaaaaac gggaguauua gucucaugug ucuugcauua     2040

ggaggaguau uaauauuuuu aaguacagca gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4678
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 1 opt

<400>  4678
gggagaaagc uuaccaugac cucaguaggu auaguagguu uacuauuaac aaccgcaaug       60

gcagcagaag ugacaagaag aggaucagca uacuauaugu auuuagauag aaaugaugca      120

ggagaggcaa uuucauuccc aacaaccuua ggaaugaaca aauguuauau acaaauaaug      180

gauuuaggac auacauguga cgcaacaaug aguuacgaau guccaaugcu agacgaaggu      240

guagaaccag augacguaga cugcuggugu aauacaacau caacaugggu uguuuacgga      300

accugucauc auaagaaagg ugaagcaaga agaaguagac gagccguaac ucuaccauca      360

cacucaacca gaaaacucca aacaagaagu cagacauggc uugaaagccg agaauacacu      420

aaacauuuaa uaagaguuga aaauuggauu uuuagaaacc caggauucgc auuagcagca      480

gcagcaauag cgugguuacu agguucauca acgucacaaa aaguuauuua uuuagucaug      540

auucuguuaa uagcaccagc uuacaguaua agauguaucg gaguaucaaa uagggacuuu      600

guugaaggaa uguccggagg uacuugggua gauguaguau uagaacaugg aggaugugua      660

acaguaaugg cacaagacaa accaaccgua gauauagagc uaguaacuac uacaguaucc      720

aauauggcag aaguacguag uuauuguuau gaagccagca uaagugauau ggcaucagau      780

ucaagaugcc caacacaagg ugaagcauau uuagauaaac aaagugacac acaauacgua      840

uguaaaagaa cauuaguaga cagaggaugg ggaaaugguu guggauuauu cgggaaggga      900

ucacuuguaa cuugugccaa auucgcaugc ucaaagaaaa ugacagggaa aucgauacaa      960

ccagaaaauu uagaauacag aauaaugcuc aguguacaug gaagccaaca uucaggaaug     1020

aucguuaaug auacugguca ugaaacagac gaaaacagag ccaaaguuga aauaacaccu     1080

aauucaccaa gagcugaagc aacauuagga ggauucggau cauuaggucu agacugugaa     1140

ccaagaacag gucuagacuu uagcgauuua uauuaccuaa cuaugaauaa uaaacauugg     1200

cuaguucaua aagaaugguu ucaugauaua ccacucccuu ggcaugcagg agcagacaca     1260

ggaacaccac auuggaauaa uaaagaagcc uuaguagaau uuaaagaugc acaugcuaaa     1320

agacagacag uuguuguacu cggaucucaa gaaggagcag uacauacagc auuagcagga     1380

gcauuagaag cagaaaugga uggugcaaaa gguagauuau cuagugguca ccuuaaaugu     1440

cgauuaaaaa uggauaaauu aagauuaaaa gggguuucau auagucuuug cacugcugcg     1500

uuuacauuua cuaaaauacc agcagaaacu cuacacggca caguaacugu agaaguacaa     1560

uaugcuggaa cugacggccc cuguaaagua ccagcgcaaa uggcugucga uaugcaaacc     1620

cuaacacccg uaggaaggcu gauaacagca aauccuguaa uaacagaaag uacggaaaau     1680

ucaaaaauga uguuagaacu agauccacca uucggcgauu cauacauagu aauuggagua     1740

ggagaaaaga aaauaaccca ccauuggcau aggagcggaa gcacaauagg aaaagcauuc     1800

gaagcaacag uaagaggugc aaaaagaaug gcaguacuag gagauacagc uugggacuuu     1860

ggauccguug guggagcauu aaauucguua gguaaaggaa uacaccaaau uuucggagca     1920

gcuuucaaau cccuauuugg aggaauguca ugguuuuccc aaaucuuaau aggaacacua     1980

uuaauguggc uaggccuaaa ugcuaaaaau ggaucuauau cauuaaugug cuuagcacua     2040

ggcggcguuc ugauauuuuu aagcacugca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4679
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 1 opt

<400>  4679
gggagaaagc uuaccaugac aaguauagga aucauuggau uacuacuaac aacugcaaug       60

gcagcagaaa uaacuagacg aggaucagca uauuacaugu auuuagauag auccgaugcu      120

ggcaaagcaa uuucauuugc aacaacacua ggaguaaaca aaugucaugu ucaaauaaug      180

gaucuaggac auauguguga ugccacaaug ucguaugagu guccaauguu agaugaaggu      240

guagaaccag acgauguaga uugcuggugu aauacgacau ccacaugggu aguauaugga      300

acuugucauc acaagaaagg agaggcaaga cgcuccagga gagcaguuac auuaccaagu      360

cauaguacaa gaaaauuaca aacaagaagc caaacuuggc uugaaucgag agaauauaca      420

aaacauuuaa uuaaaguaga aaauuggaua uuuagaaacc caggauucgc auuaguggca      480

gucgcaauug ccuggcuauu agggagcagu acaucacaaa aaguaauaua ucucguaaug      540

auacuauuaa uagcuccugc auacagcaua agaugcauag gaguuucaaa ccgagauuuu      600

guagaaggua ugagcggagg cacaugggua gacguuguac uagaacaugg aggauguguc      660

acugugaugg cacaagacaa accaacagua gauaucgaac ugguaacaac aacaguaagu      720

aauauggcag aaguaagauc cuauugcuau gaagcaucga uaucagacau ggcaagugau      780

ucuagaugcc caacucaagg agaagcauau uuagauaaac aaagcgacac acaauaugua      840

ugcaagagga cucuaguaga uagagguugg gguaauggau guggcuuauu uggaaaagga      900

agucucguaa caugcgcaaa guucacaugu ucaaagaaaa ugacuggaaa aucuauacaa      960

ccagaaaauu uagaguauag aauaaugcuc agcguacaug guucacaaca caguggaaug     1020

aucggauacg aaacugacga agaccgagca aaaguugaag uaaccccuaa uucaccaaga     1080

gcagaagcaa cauuaggagg guucggaagc cucggauuag acugcgaacc acgaacagga     1140

uuagauuuuu cagaucuaua cuauuuaaca augaacaaca aacauugguu aguucauaaa     1200

gaaugguuuc augauauacc acuaccaugg caugcaggcg cugauacagg cacuccacau     1260

uggaauaaca aagaagcauu aguagaauuu aaagacgcac acgcaaaacg acaaacagua     1320

guaguuuuag gaagccaaga aggagcggua cacacugcau uagccggugc auuagaagca     1380

gaaauggaug gugcaaaagg aagacuauuu ucuggucauu uaaaaugucg auuaaaaaug     1440

gauaaauuaa gacucaaagg aguaucuuac aguuuaugua cagcagcauu cacauucaca     1500

aaaguaccug cagaaacauu acauggaacg guuacaguag aagugcaaua ugcuggaaca     1560

gauggaccau guaaaauacc agugcaaaug gcuguagaua ugcagacuuu aacaccaguc     1620

ggaagauuaa uaaccgcaaa uccaguaaua acagaaagua ccgaaaauuc aaaaaugaug     1680

uuagaauuag auccaccauu uggcgacagu uauauuguua uaggaguagg cgauaagaaa     1740

auaacacauc acuggcauag aagcggaucu acaauaggaa aagccuuuga agcaacagua     1800

agaggagcca aaagaauggc aguacucggu gauacagcuu gggauuucgg aucuguugga     1860

ggaguuuuca auucauuagg caaaggaaua caucaaauau ucggagcugc cuuuaaauca     1920

uuauuuggug gaaugucaug guuuucucaa auucuaauag guacauuacu uguauggcua     1980

ggacuaaaca cuaaaaaugg uucaauauca cuaacauguc uagcuuuagg cggaguaaug     2040

aucuucuuaa gcacagcugu aucagcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4680
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 1 opt

<400>  4680
gggagaaagc uuaccaugac aucaguagga auagucggac uuuuguuaac gacagccaug       60

gcagcugaag uaacaagaag aggcucugca uacuauaugu aucuagaccg gaacgacgcc      120

ggagaagcaa uaucauuccc aacaacauua ggcaugaaua aaugcuacau acaaauuaug      180

gaucuuggac auaugugcga ugcaacaaug ucuuaugaau guccaauguu ggaugaagga      240

guugaaccug acgauguaga cugcuggugc aacacaacau caacaugggu uguauauggu      300

accugucauc auaaaaaagg cgaagccaga agaucaagaa gggcaguaac acugccuagu      360

cauaguacua gaaaauuaca aacuagauca caaacuuggu uagaaucaag ggaauacaca      420

aaacaccuaa uacgaguaga aaauuggaua uuucgaaauc caggguucgc acuagcagca      480

gcugcaauag cauggcuuuu aggaaguuca acaagccaaa aagucauaua ucuaguuaug      540

auucuacuua uagcaccugc auacucaaua cgaugcauag gagucucaaa uagggauuuu      600

guagaaggua ugucaggagg gaccugggua gacguaguac uagaacaugg uggaugugua      660

acagucaugg cacaagauaa accaacagua gacauagaau uaguaacaac gacugucucc      720

aauauggccg aaguuagauc auacugcuac gaagcuucua uaucagauau ggcaagcgau      780

ucaagauguc ccacccaagg ugaagcauau cuagacaaac aaucagauac acaauacguu      840

uguaaaagaa cauuaguaga cagagguugg ggaaauggau guggauuauu uggaaaaggu      900

aguuuaguaa caugcgcaaa auuugcuugc agcaaaaaaa ugacaggaaa auccauacaa      960

ccagaaaacc uugaguauag aauaaugcuc aguguucaug gaucacaaca uucuggaaug     1020

auaguaaaug acacaggaca cgaaacugac gagaaucgug cuaaaguaga aauaacccca     1080

aauaguccua gagcagaagc aacauuaggc ggauuuggaa guuuaggauu agauugugaa     1140

ccaagaacag gcuuggauuu uagugaucua uauuacuuaa caaugaauaa caaacauugg     1200

uugguccaua aagaaugguu ccaugauaua ccauuaccau ggcacgcugg agcagauacu     1260

ggaacaccgc auuggaauaa uaaagaagca cuuguugaau ucaaagacgc ucaugcuaaa     1320

cgacaaacag uaguuguacu cggaagccaa gaaggcgcag uccacacagc ucuagcagga     1380

gcguuagaag cugaaaugga uggcgcaaag ggaagauuaa gcucaggaca ccuaaaaugc     1440

agacuuaaaa uggacaaacu aagauuaaaa ggaguuaguu auagccuaug uacagcagca     1500

uucacauuua cuaaaauacc agcugaaaca uuacauggaa cuguuacagu agaaguacaa     1560

uaugcaggaa cagauggucc auguaaagua ccugcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccug ucggaagauu aauuacagca aauccuguaa uaacggaaag uaccgaaaau     1680

ucaaaaauga ugcuagaauu agacccaccu uuuggcgaua gcuacauagu aauuggagua     1740

ggagaaaaaa aaauaacaca ucauuggcac cgaaguggaa gcacaauagg aaaagcauuu     1800

gaagcaacag uuagaggagc aaaaagaaug gcaguauuag gggauaccgc cugggauuuc     1860

gguucuguag gaggugcacu aaauagucuu ggaaaaggca uacaccaaau uuuuggagca     1920

gcuuucaaau cacuguuugg uggaaugagu ugguuuucac aaauucuaau aggaacauua     1980

uuaauguggu uaggauuaaa uacaaaaaac ggaucaauaa gcuuaaugug ucuagccuua     2040

ggaggcguau uaauauuccu aucgacagcc guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4681
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 opt

<400>  4681
gggagaaagc uuaccaugag ucaaaaagua auauaucuag ucaugauauu auuaauagca       60

ccagcauauu caauaagaug uauaggaguc ucaaacaggg auuucguaga aggaaugagu      120

ggagguacgu ggguagauau aguuuuagaa cauggaggau guguaaccgu uauggcacaa      180

gacaaaccaa caguagacau agaguuaguc acaacaacag uaagcaauau ggcagaagua      240

agaucauacu guuaugaagc aaguauaagu gauauggcaa gugacucaag auguccaacu      300

cagggagaag cauaccuaga caaacaauca gacacacaau acguauguaa acgaacacua      360

guggauagag gauggggaaa ugguugugga cuauuuggaa aaggaagccu gguuacaugc      420

gcuaaauuug ccugcucaaa aaaaaugaca ggaaaaucaa uacaaccaga aaaucuagaa      480

uaucggauua uguuaagcgu acauggaagc caacacucag gaaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa ucgagcaaaa guggaaauaa ccccaaacag cccaagagca      600

gaagcuacac uaggaggauu uggauccuua ggacuagauu gugaacccag aacaggacua      660

gacuuuucug auuuauacua ucuuacaaug aauaauaaac auugguuagu acacaaagaa      720

ugguuccaug auauaccauu accauggcau gcaggagcag auacgggcac accacacugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaaagaca aacaguagua      840

guauuaggau cacaagaagg cgcaguucau acagcccuag caggagcacu agaagcagaa      900

auggauggag caaaaggaag gcuuagcagc ggucauuuaa aaugcagauu aaaaauggau      960

aaauuaagac uaaaaggagu aaguuauagu cuauguacag ccgcauuuac auuuacaaaa     1020

auaccagccg aaacuuuaca uggaacagua acaguagagg uacaauaugc aggaacggac     1080

ggaccaugua aaguaccagc acaaauggcu guagacaugc aaacacuaac cccaguaggc     1140

agauuaauaa cagcuaaucc aguuauaaca gaaaguacag aaaauucuaa aaugaugcua     1200

gaauuagacc caccuuuugg agacucauau auaguaauag guguaggaga aaaaaaaaua     1260

acacaucauu ggcauagauc aggaaguaca auaggaaaag cauucgaagc aaccguaaga     1320

ggagccaaaa gaauggcggu acuaggugau acagcauggg auuuuggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4682
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 opt

<400>  4682
gggagaaagc uuaccaugag ucaaaaaguu auauauuuag uuaugauuuu acuuauagcu       60

ccggcauaca guauaagaug uauaggagua ucaaauagag auuucguaga gggaaugagu      120

ggaggaacau ggguagaugu aguauuagaa cauggagguu guguaacagu aauggcacaa      180

gacaaaccaa caguagauau agaauuaguu acaaccacag uaucaaauau ggcagaagua      240

agaagcuauu gcuaugaagc aaguauaagu gacauggcaa gugauagcag auguccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacacaau acguuuguaa aagaacauua      360

guagauagag guuggggaaa cggauguggu uuauuuggaa aagggucuuu aguaacuugu      420

gcgaaauuug cuugcaguaa aaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa      480

uauaggauaa uguuaucagu acauggaucu caacauagcg guaugauagu aaaugacaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccgaauuc gcccagagca      600

gaagcaacac ugggaggauu uggaagccua ggacuagauu gugaaccaag aacgggacua      660

gauuuuucag aucuguauua ucuuacaaug aauaauaaac auuggcuagu gcauaaagag      720

ugguuucaug auauaccacu accauggcau gcaggagcag acacagguac accacauugg      780

aauaauaaag aagcacuagu agaauucaaa gacgcacaug caaaaagaca aacaguuguu      840

guacuaggaa gccaagaagg agcaguacau acagcauuag caggggcauu agaagcagaa      900

auggacggag caaaagguag acuaucgagc ggacaccuaa aauguagauu aaaaauggau      960

aaacuacgau uaaaaggagu aaguuacagu uuaugcacag cugcauuuac auuuacaaaa     1020

auaccggcag aaacauuaca uggaacgguu acaguagaag uacaauacgc uggaacagau     1080

ggacccugua aagugccugc gcaaauggca guggacaugc aaacauugac accaguagga     1140

agacuaauaa cagcaaaucc aguaauaaca gaaaguacgg aaaauaguaa aaugauguua     1200

gaacuagauc cuccauuugg agacucauau auaguaauag gaguaggaga aaaaaaaauc     1260

acacaccacu ggcacagauc aggaucgaca auaggaaaag ccuuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcugu auuaggggac acagcauggg acuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4683
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 opt

<400>  4683
gggagaaagc uuaccauguc acaaaaagua auuuaucuag uuaugauauu auuaauagcu       60

ccagcauaca guauaagaug uauaggagua ucaaacagag acuuuguaga aggaauguca      120

ggaggaacau ggguugacgu aguauuagaa cacgguggau gcguaacagu aauggcacag      180

gacaaaccaa caguugauau agaauuaguu acaacaacag uuucaaauau ggcugaagua      240

agaucauauu guuaugaagc aaguauaagu gacauggcca gcgacaguag augcccuaca      300

caaggagaag cauaccuaga uaaacaaagc gauacacaau acgucugcaa aagaacccua      360

guagacagag guuggggcaa cgguugugga cuauuuggua aagguagcuu gguaacaugu      420

gcaaaauuua cauguucaaa gaaaaugaca ggaaaaucaa uacaacccga aaaucuugaa      480

uauagaauaa ugcugagugu acauggaagu caacauucag gaaugaucgg auacgaaaca      540

gacgaagaua gagcaaaagu agaaguaaca ccaaauucac cgagagcaga agcaacacua      600

ggaggauuug gcucacuagg auuagacugu gaaccaagaa caggacuaga uuucagugau      660

cuauacuacc uaacaaugaa caacaaacac ugguuaguac auaaagaaug guuucaugac      720

auaccauuac cuuggcacgc aggagcagau acaggaacac cacauuggaa uaauaaagaa      780

gcauuagugg aauuuaaaga ugcucaugca aaaagacaaa caguaguagu acuaggaagu      840

caagaaggcg ccguacacac agcucuagca ggagcauuag aagcagaaau ggauggagca      900

aaaggaagau uauuuagugg acacuuaaaa uguagauuaa aaauggauaa acuaagauua      960

aaagguguau cauauaguuu auguacagca gcuuucacau uuacaaaagu accagcagaa     1020

acauuacaug gaacagucac aguagaagua caauacgcag gaacagaugg accauguaaa     1080

auaccaguac aaauggcagu agacaugcaa acacuaacuc caguaggaag guuaauaaca     1140

gcaaauccag uaauaacaga aaguacagaa aauaguaaaa ugauguuaga auuagauccc     1200

ccauuuggag acaguuauau agugauaggc guaggagaca agaaaauaac acaucauugg     1260

cauagaucag guucaacuau aggaaaagca uuugaagcua caguaagagg ugcgaaaaga     1320

auggcaguac uaggagauac agcaugggac uuuggaaguu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4684
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 1 opt

<400>  4684
gggagaaagc uuaccaugag ucaaaaaguu auauauuugg uaaugauauu acuaauugcu       60

ccagcauauu caauacgaug cauaggagua ucaaauagag auuucgucga aggaaugagu      120

ggaggaacau ggguagaugu aguauuagaa cauggaggau gcguaacagu aauggcacaa      180

gauaaaccaa caguagauau agaacuagua acaacaaccg uaucaaacau ggcagaagua      240

agaucauauu guuaugaagc aucaauaagc gacauggcgu cugauaguag augcccaaca      300

caaggugagg cauauuuaga uaaacaaagc gauacacaau auguuugcaa aagaacauua      360

gucgauagag gauggggaaa uggaugugga uuguucggaa aaggaucacu agucacaugu      420

gcaaaguuug cauguaguaa aaaaaugaca ggaaaaagua uccaaccaga aaacuuagaa      480

uauagaauaa ugcucagugu acaugguagu caacauagug gcaugauagu gaaugauaca      540

ggucaugaaa cagaugaaaa ucgagcgaaa guagaaauua cacccaauuc uccuagagca      600

gaagcaacuu uaggaggauu uggaaguuua ggauuagauu gcgaaccaag aacaggauua      660

gacuucagcg aucuauacua uuuaacaaug aacaauaaac auuggcucgu acauaaagaa      720

ugguuucaug acauaccucu accuuggcac gcaggagcag auacaggaac uccacauugg      780

aauaauaaag aagccuuagu agaauuuaaa gaugcacaug caaaaagaca aacaguaguc      840

guacuaggaa gccaagaagg agcaguacau acugcauugg caggagcauu agaagcagaa      900

auggacggug caaaaggaag guuaucaagu ggacaucuaa aaugcagacu uaaaauggau      960

aaauuaagau uaaaaggagu aaguuauuca uuaugcacag cagcauuuac auuuacaaaa     1020

aucccagcag aaacacuaca uggaacagua acaguagaag uacaauacgc aggaacugau     1080

ggaccaugua aaguaccagc acaaauggcc guugauaugc aaacauuaac accaguagga     1140

agauuaauaa cagcaaaucc aguaauaaca gaaaguaccg aaaacaguaa aaugauguua     1200

gaacuagauc cuccauuugg agacaguuac auaguuauag gaguaggaga aaaaaaaaua     1260

acacaccauu ggcacagaag uggaagcaca auagguaaag cauuugaagc aacaguaagg     1320

ggagcaaaaa gaauggcagu acuaggagau acagcauggg auuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4685
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       1 opt

<400>  4685
gggagaaagc uuaccaugac aucaguagga auuguuggau uauuacuaac aacagcaaug       60

gcagcagaag uaacaagacg aggaagcgca uauuauaugu aucuggauag aaaugaugcu      120

ggugaagcaa uaaguuuucc aacaaccuua gguaugaaua aauguuacau ucaaaucaug      180

gauuuaggac acauguguga ugcaacaaug ucauaugaau guccuaugcu agaugaaggc      240

guugaaccag acgaugugga uuguuggugc aacacaacaa guacaugggu gguauauggu      300

acgugucauc acaagaaagg agaagcaaga agaucaagga gagcaguuac acuuccaagc      360

cauaguacaa gaaaauuaca aacaagaagu caaacauggu uagaaucaag agaguacaca      420

aaacaccuaa uuagaguaga aaauuggaua uucagaaauc caggguucgc acucgcagca      480

gcagcuauag caugguuacu aggaaguagu acaagucaaa aaguaauaua ucuaguaaug      540

auacuauuga ucgcgccagc auauucuaua agauguauag gaguaagcaa uagagauuuu      600

guagaaggua ugucuggagg uacaugggua gauauaguau uagaacacgg cggaugcguc      660

acaguaaugg cccaagauaa accaacagua gauauagaac ucguaacaac aacaguuagu      720

aauauggcag aaguaagaag cuacuguuac gaagccagua uaucagacau ggcaagugau      780

ucaagauguc caacacaggg agaagcauau uuagauaaac aaucugacac acaauauguu      840

ugcaaaagaa ccuuaguaga cagaggaugg ggaaacggau gcggacuaag cggaaaaggu      900

agcuuaguaa caugugcaaa auucgcaugc ucaaagaaaa ugacuggaaa aagcauacaa      960

ccagaaaacu uagaauauag aauuauguua aguguacaug gaucacaaca caguggaaug     1020

auaguaaacg acacuggaca ugaaaccgau gaaaaucgag caaaaguaga aauaacaccu     1080

aauagcccga gagcggaagc cacacuagga ggauucggaa guuuaggacu agacugugaa     1140

ccaagaacag gauuagacuu uagugaucua uauuauuuaa ccaugaacaa uaaacauugg     1200

uuaguucaua aagaaugguu ccaugacaua ccauuaccau ggcacgcagg agcagauacc     1260

gguacgccgc auuggaauaa caaagaagcu cuaguagaau uuaaagaugc acaugcaaaa     1320

cgacaaacug uagugguacu aggaucacaa gaaggagcag uucauacagc uuuagcgggg     1380

gcgcucgaag cagagaugga cggugcaaaa gguagauuaa guucaggaca ccuaaaaugu     1440

agacuaaaaa uggauaaacu aagauuaaaa ggaguaucgu auucuuuaug uacagcugcg     1500

uuuaccuuua caaaaauucc agcagaaaca uuacauggaa ccgucacagu ugaagugcaa     1560

uacgcaggaa cugauggucc augcaaaguc ccugcacaga uggcaguaga uaugcaaacg     1620

cuaacaccgg uaggacgauu gaucacugcg aauccuguga uaacugaaag caccgaaaau     1680

ucaaaaauga ugcuggaauu agauccucca uuuggagaca guuacauagu aauaggagua     1740

ggagaaaaga aaauaacaca ucauuggcac agaucaggaa guacaauagg caaagcauuu     1800

gaagcuacag ugaggggagc aaaacgaaug gcaguauuag gugauacagc augggacuuu     1860

ggcaguguag gaggggcacu aaauucuuug ggaaaaggua uacaucaaau cuuuggagca     1920

gcauuuaaaa guuuauuugg aggaaugagc ugguuuucac aaauuuugau aggcacuuua     1980

uuaauguggu uaggacuaaa uacuaaaaau ggaucaauau cauuaaugug uuuagcuuua     2040

ggaggcguau uaauauuuuu auccacagca gugagcgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4686
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 1
       opt

<400>  4686
gggagaaagc uuaccaugac auccguagga auuguaggau uauuacuaac aaccgcaaug       60

gcagcagaag uaacgcgacg aggaagugca uauuacaugu acuuagauag aaaugacgcu      120

ggugaagcaa uaaguuuucc uacaacucuu gggaugaaca aauguuacau ccaaauaaug      180

gaucuuggcc auacauguga ugcaacaaug ucauaugaau guccaauguu agaugaagga      240

gucgaacccg acgaugucga uuguuggugc aauaccacau caacaugggu uguauaugga      300

acaugccauc auaagaaagg agaagcgcgg agaaguagac gagcaguaac auuaccauca      360

cauucgacac gaaaauuaca aacaagaagu caaacauggu uagaaagcag agaauacaca      420

aaacauuuaa uaagaguaga aaacuggaua uuuagaaauc caggguuugc auuagcagca      480

gcagcaauag cauggcuucu aggauccagu accucacaaa aaguaauaua cuuaguaaug      540

auauuauuga uugcaccagc uuauucuaua agauguauug gaguuagcaa uagagauuuu      600

guagaaggga ugaguggagg aacaugggua gauguaguuu uagaacacgg agguugugua      660

acaguaaugg cacaagauaa accaacagua gauauagagc uaguaacaac uacugugagc      720

aauauggcag aaguucgauc auacuguuac gaagcaucaa uaagugacau ggcaucggau      780

agcagauguc caacacaagg ugaagcauau cuagauaaac aaucagauac ucaauaugua      840

uguaaacgaa cuuuaguaga uagaggaugg ggaaauggau gcgguuuaag cggaaaaggc      900

ucauuaguua caugugcuaa auucgcuugu aguaagaaaa ugacaggaaa aucaauacaa      960

cccgaaaauc uagaauaucg aauaauguua uccguacaug gaagccaaca uucagguaug     1020

auaguaaaug acacaggaca ugaaacagac gaaaauagag cuaaaguaga aauaacuccu     1080

aauucaccaa gagcagaagc uacacuagga gguuucggua gccuaggguu agauugugaa     1140

ccaagaacag gauuagacuu uucagacuua uauuaccuaa caaugaauaa uaaacauugg     1200

uuaguucaca aagaaugguu ucacgauaua ccauugccuu ggcaugcagg agcagauacu     1260

ggaacaccac acuggaauaa uaaagaagca uuaguagaau uuaaagaugc acacgcaaaa     1320

agacagacag uugucguauu agggucacaa gaaggagcug uacauacagc auuagccgga     1380

gccuuagaag cagaaaugga cggagcaaaa ggaagacuaa guagcggaca ucuuaaaugu     1440

agacuaaaaa uggacaaacu acgacugaaa ggaguaucau auucauuaug uacagcagcu     1500

uuuacauuca caaaaauacc agcugaaacc cuacauggaa caguaacagu cgaaguucaa     1560

uacgcaggaa cggacggacc auguaaagua ccagcucaaa uggcaguaga caugcagacu     1620

uuaacaccag uagggaggcu aauuacagca aacccuguua uaacagaauc aacggaaaac     1680

ucaaaaauga uguuggaauu agauccaccu uuuggagacu cauacauagu aauaggagua     1740

ggagaaaaga aaauaacaca ucacuggcau cgaaguggau caacuauugg uaaagcauuu     1800

gaagcaaccg uaagaggagc uaaaagaaug gcuguuuuag gugauacagc gugggauuuu     1860

ggcucagugg gaggugcauu aaacucuuua ggcaaaggga uacaccaaau auuuggugcc     1920

gcauuuaaau cauuauucgg aggcauguca ugguucagcc aaauauuaau aggaacauua     1980

uuaauguggu uaggucuaaa cgcaaagaac ggaaguauuu cucuaaugug uuuagcauua     2040

ggaggaguuc uaauauuucu aucaaccgca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4687
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 1 
       opt

<400>  4687
gggagaaagc uuaccaugac aucaauugga auaaucggcu uauugcuaac uacagcaaug       60

gcagcugaaa uuacaagaag aggaucagcu uauuauaugu aucuagacag aucagaugcu      120

gguaaagcaa uuucauucgc aacaacacua ggcguaaaua aaugucacgu acaaauaaug      180

gauuuaggcc auaugugcga ugcaacaaug ucguaugaau guccaauguu agacgaagga      240

guggaacccg augauguaga cuguuggugc aacacaacuu caacaugggu aguauacgga      300

acaugccauc acaaaaaagg agaagcaaga agaucaagac gagcaguuac ucuaccaagc      360

cauaguacaa ggaaacuaca aaccagauca caaacauggc uagaaucacg ugaauacaca      420

aagcauuuaa uaaaaguaga aaacuggaua uuuagaaauc caggguucgc auuaguggca      480

guagcaauag cauggcuauu aggaucauca acaucacaaa aaguaauaua uuuaguaaug      540

auauuauuaa uagcgccagc auauaguaua cgauguauag gaguaucaaa cagagauuuu      600

guggaaggaa ugucaggagg uacaugggug gacguaguuc uagaacacgg aggcugugua      660

acaguaaugg cacaagacaa accuaccgua gauauagaac uagugaccac aacaguauca      720

aacauggcgg aaguaagguc uuauugcuau gaagccagua uaucagauau ggcaucugau      780

agcagaugcc caacacaagg agaagcauau cuugauaaac aaucugauac acaauaugua      840

uguaaaagaa ccuuaguaga ucgaggaugg ggaaaugguu guggacuauc agguaaaggu      900

ucauuaguca caugugcuaa auucacuugu aguaaaaaaa ugacaggaaa aucuauacaa      960

ccagaaaauc uugaauauag gauaauguua aguguccacg gaagucaaca uucagggaug     1020

auagguuaug aaacagauga agacagggca aaaguagaag uaacaccaaa cagcccaaga     1080

gcggaagcaa cauuaggagg auucggaagc cucggacuag acugugaacc acggacggga     1140

uuagacuuca gcgaucuuua uuauuuaaca augaauaaua aacacuggcu aguucauaaa     1200

gaaugguucc augauauccc ucuaccaugg caugcaggag cagauacagg aacaccccac     1260

uggaauaaua aagaagccuu aguagaauuu aaagaugccc acgccaaaag acaaacggua     1320

guuguauuag gaucacaaga aggagcagua cauacggcgu uagcuggcgc ccuagaagca     1380

gaaauggaug gugcgaaagg aagacuauuu ucaggccauc ucaaaugcag auugaaaaug     1440

gauaaacuua gacuaaaagg aguaucauau ucauuaugua cagcagcauu uacauuuacc     1500

aaaguaccag cagaaacauu acauggaaca guuacaguag aaguucaaua ugcagguaca     1560

gauggaccuu guaagauacc aguacaaaug gcgguagaua ugcaaacacu cacuccaguu     1620

gguagauuaa uuacugcaaa cccaguaaua acagaaucga cggaaaauuc uaaaaugaug     1680

uuagaauuag acccaccauu uggagauagu uauauaguga uagguguggg agauaaaaaa     1740

auaacacauc auuggcaucg aucaggguca acuauaggua aagcauucga agcaacagug     1800

agaggggcaa aaaggauggc aguacuagga gauacugcau gggauuuugg cagugucggu     1860

gggguuuuua auagccuagg uaaaggaaua caccaaauau uuggcgcagc guucaaauca     1920

uuauuuggcg gaaugucaug guuuucucaa auacuuauag gaaccuuacu aguaugguug     1980

gguuuaaaca ccaaaaaugg aaguauuuca cuaacauguu uagcccuggg aggaguaaug     2040

auauuucuaa gcacagcagu aagugcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4688
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 1 
       opt

<400>  4688
gggagaaagc uuaccaugac aucaguagga auaguaggac uacuauuaac aacagcaaug       60

gcagcugagg uaacaagaag aggaagcgcu uauuauaugu auuuagauag aaaugaugca      120

ggagaagcua ucagcuuccc aacaacacua ggaaugaaca aauguuauau acaaauaaug      180

gauuuaggcc acaugugcga ugcaacaaug agcuaugaau guccaauguu agaugaagga      240

guagaaccug augauguuga uuguuggugc aacaccacaa guacaugggu aguuuaugga      300

acaugccauc acaagaaagg ugaagcacga agaucaagac gagcaguaac acugccaucu      360

cauucaacac gaaaauuaca gacaagaagu cagacauggc uugaaaguag agaauauacg      420

aaacaccuaa uacgcguaga aaauuggaua uuuagaaacc cuggauuugc cuuggcggca      480

gcagcaauag cuugguuauu agguucuagu acaucacaaa aaguaauaua ucuaguaaug      540

auacuacuaa uagcaccagc auacucaaua agaugcauag gaguaagcaa cagagauuuc      600

guagaaggaa ugaguggagg aaccugggua gauguagucc uagagcacgg aggaugugua      660

acaguaaugg cacaagacaa accaacugua gauauagaac uuguaacaac aacgguauca      720

aacauggcag aaguacgaag uuacugcuac gaagcaucaa uaucugacau ggcuucagau      780

ucaagaugcc caacacaagg agaagccuau cuagauaaac aaucagacac acaauacguu      840

ugcaaacgaa cauuaguaga ccgagguugg ggaaacggau gugggcuauc agggaaaggu      900

ucacuuguaa caugugcaaa auuugcaugc ucaaagaaaa ugaccgguaa aaguauucaa      960

ccagaaaauc uagaauacag aauuaugcua ucaguacacg gaucacaaca uagugguaug     1020

auaguaaaug acacuggaca ugagacugau gaaaauagag caaaaguaga aauaacacca     1080

aauaguccaa gagcagaagc aacauuagga ggauuuggua guuuaggacu cgacugugaa     1140

ccacggacug ggcuggauuu uagugacuua uauuacuuaa ccaugaauaa uaaacacugg     1200

uuaguacaca aagaaugguu ccaugauaua ccuuugccau ggcaugcugg ugcagauaca     1260

ggaacucccc auuggaauaa uaaagaagca cuuguagaau uuaaagaugc acacgcaaaa     1320

cgacaaacag uaguuguacu agguucccaa gaaggagcag uacauacagc acuugcagga     1380

gcucuagaag cugaaaugga uggagcuaaa ggacgauuaa guaguggaca ccuaaaaugu     1440

agacuuaaaa uggacaaauu aagguuaaaa ggaguaaguu auucauuaug uacagcagca     1500

uucaccuuca cuaaaauacc ugcggaaaca cuccacggua caguaacugu agaaguacaa     1560

uaugcaggaa cagauggacc auguaaagua ccagcacaaa uggcugugga uaugcaaaca     1620

cuaacucccg uaggaagacu aauaacugca aacccaguaa ucaccgagag uacagaaaac     1680

ucuaaaauga uguuagaauu agauccacca uuuggagauu cauacauugu aauuggagua     1740

ggugaaaaga aaauaacuca ccauuggcau agaucuggaa guacaauagg aaaagccuuc     1800

gaagcaacag uaagaggugc uaaacggaug gcaguauuag gagauacggc augggauuuu     1860

gguucuguag guggagcacu aaacucccua gggaaaggga uucaccaaau uuuuggagca     1920

gcauuuaaau cauuauuugg aggcaugagc ugguuuucac agauacuaau agguacauua     1980

cuuauguggu uggguuuaaa cacuaaaaac gguucaauuu cauuaaugug cuuagcacua     2040

gguggugugu uaauauuucu aaguacagcg guuagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4689
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       1 opt

<400>  4689
gggagaaagc uuaccaugac cagcguagga auuguagggc ucuuauuaac aacagcuaug       60

gcagcagaag ugacaagaag ggguagugcc uauuacaugu acuuagauag aaaugacgca      120

ggugaagcaa uuucauuucc aacaacauua ggaaugaaua aauguuacau acaaauaaug      180

gaccuaggac auauguguga cgcaacaaug aguuaugaau gucccauguu agacgagggg      240

guugaaccag augauguaga uugcuggugu aauacaacga guacgugggu aguauaugga      300

acgugucauc auaaaaaagg agaagcucgu agaaguagga gagcaguaac uuuaccaagu      360

cacucaacaa gaaaauuaca aacaagaagc caaacauggu uagaaucaag agaauauaca      420

aaacaucuca uaagaguaga aaauuggaua uuuagaaauc caggauuugc auuagcagcu      480

gcugcaauug ccugguuacu cggaaguucc accucacaaa aaguaauaua ccucguaaug      540

aucuuguuaa uagcaccagc uuauucgaua agauguaucg gaguuaguaa uagagauuuu      600

guugaaggca ugagcggagg gacaugggua gauauaguac uugaacaugg cggauguguu      660

acaguaaugg cacaagauaa accaacggua gauauugaau uaguaacaac aacagucagu      720

aacauggcug aaguaagauc auauugcuau gaagcuagua uaucugauau ggcuagugau      780

aguagauguc caacucaagg agaagccuau uuagauaaac aaagugacac ucaauauguu      840

uguaaaagaa cauuaguaga cagaggaugg ggaaaugguu guggauuauu cggaaaagga      900

agcuugguaa caugugcaaa auucgcaugu aguaaaaaaa ugacaggaaa aucuauucaa      960

ccagaaaacu uagaauauag gauaauguua ucagugcacg gcucacaaca uaguggaaug     1020

auaguacaag acacagggca ugaaacagau gaaaauagag caaaaguaga aauaacucca     1080

aacaguccua gagcugaagc aacucuaggu ggauuuggaa guuuaggcuu agauugugaa     1140

ccaaggacag gauuagauuu uagugauuua uauuaccuaa caaugaauaa uaaacauugg     1200

cuuguacaua aagaaugguu ccaugauauu ccacuaccau ggcaugcagg agcagacacu     1260

gguacuccac auuggaauaa caaagaagca uuaguugaau uuaaagaugc ccaugcuaaa     1320

agacaaacag uaguaguauu aggaagucaa gaaggagcug uucacacugc acuugcagga     1380

gcacuagaag cagaaaugga uggagcaaaa ggacgguuaa gcaguggaca uuuaaaaugc     1440

agacuaaaaa uggauaaacu ucgauuaaaa gggguaucau auaguuuaug uacugcagcu     1500

uuuacguuua caaaaauacc agcggaaaca cuacauggaa caguuacagu ugaaguacag     1560

uaugcaggca cugauggacc uuguaaggua ccagcgcaaa uggcaguaga caugcaaaca     1620

cuaacuccag ugggacgacu aauaacagca aauccaguaa uaacagaaag cacagaaaau     1680

aguaaaauga ugcuagaacu agacccacca uuuggagacu cauacauagu cauaggcgua     1740

ggagaaaaaa aaauaaccca ucauuggcac agaaguggau caacuauagg aaaagcauuu     1800

gaagcaacag uucguggagc aaagagaaug gcaguauugg gagacaccgc augggauuuc     1860

gguaguguug gaggugcacu uaauucauug ggaaaaggaa uacaucaaau uuuuggagca     1920

gcuuucaaaa guuuauucgg cgggauguca ugguucaguc aaauauuaau agguacuuua     1980

cuaauguggu uaggauuaaa uacaaaaaau gguagcauuu cacuuaugug ucuagcauua     2040

ggagguguuu ugauauuucu aucaacagcu gugucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4690
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 1
       opt

<400>  4690
gggagaaagc uuaccaugac aucagucgga auagucggac uucuauuaac uacugcaaug       60

gcagcagaag ucacaagaag aggaucagcu uauuacaugu acuuagauag gaaugaugcu      120

ggagaagcca uuagcuuucc caccacuuua ggcaugaaua aauguuacau acaaauaaug      180

gauuuaggac auacauguga ugcaacaaug ucauacgaau gucccauguu agaugaaggu      240

guagaaccag augauguuga uuguuggugc aauacaacau caacaugggu aguauacgga      300

acaugccauc auaagaaagg agaagcaagg cgaucaagac gagcaguuac auugccuagu      360

cauaguacca gaaaauuaca aacaagaucu caaacauggu uagaaaguag agaauacaca      420

aaacaucuaa uaagaguuga aaacuggaua uucagaaauc caggauuugc acuagcagca      480

gcagccauag cauggcuauu aggaucaagc acaagucaaa aaguuauaua cuuagugaug      540

auauuauuaa uagcaccagc uuacucuaua agauguaucg gagucuccaa cagagauuuu      600

guagaaggaa ugucaggagg aacauggguu gauguaguau uagaacaugg uggaugugua      660

acaguaaugg cacaagauaa accaaccgua gauauagaau uagucacaac aacuguauca      720

aauauggccg aaguaagaag uuacuguuau gaagcaucaa uuucugacau ggcuucagau      780

agccgcugcc ccacacaagg cgaggcauau uuagacaaac aaagcgauac acaauaugua      840

uguaaaagaa cucuaguuga uagaggaugg gguaauggau guggacuauu cgguaaaggu      900

ucauuaguaa caugugcaaa auuugcuugu agcaagaaaa ugacagggaa aucaauccaa      960

ccagaaaauc uagaauauag aauaauguua ucagugcacg gaagucagca cucagguaug     1020

auaguucaag auacaggaca ugaaacagac gaaaauagag caaaagugga aaucacgcca     1080

aauucaccaa gagcugaagc uacccuaggu ggcuuuggau cauuaggcuu agauugcgaa     1140

ccaaggacag gauuagauuu uucagaucuc uacuauuuaa caaugaauaa uaaacacugg     1200

cuaguccaca aagaaugguu ccaugauauc ccauugccuu ggcaugcugg agcagauaca     1260

gguacaccac auuggaauaa caaagaagca cucguagagu ucaaagaugc acaugcaaaa     1320

agacaaacag uaguaguacu aggaucccaa gaaggugcag uucacacugc acuggcagga     1380

gccuuagaag cagaaaugga uggagcaaaa ggaagauuau caaguggaca ccuaaaaugu     1440

agauuaaaaa uggauaaacu aagauuaaaa ggaguauccu auucccucug caccgcagcc     1500

uucacuuuua cuaaaauacc agcagaaaca uugcauggaa caguuaccgu agaaguacaa     1560

uacgcaggaa cugauggacc cuguaaagua cccgcacaaa uggcaguaga uaugcaaaca     1620

cucacaccag uaggaagauu aauaacagca aauccaguaa uaaccgaauc aacagaaaac     1680

agcaaaauga ugcuugaauu agauccaccu uuuggagauu cauauauagu aauuggugua     1740

ggcgaaaaga aaaucacaca ucauuggcau agaaguggau caaccauagg aaaagcauuu     1800

gaagcaacag uaagaggggc aaaacgaaug gcgguccuag gagacacggc augggauuuu     1860

ggaucaguag gaggagcauu aaauucacua ggaaaaggca uucaucaaau auucggagcu     1920

gcauucaaaa guuuauucgg aggaauguca ugguuuaguc aaauacuuau aggcacauua     1980

uuaauguggc uaggauuaaa cgccaaaaau gggucaauau cacucaugug uuuagcuuua     2040

gguggaguau uaauauuuuu auccacagca guaucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4691
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 1 
       opt

<400>  4691
gggagaaagc uuaccaugac aagcguggga auaguuggac uacuauuaac aacagcaaug       60

gcagcagaag ugacucgucg gggaagugca uauuauaugu auuuagauag aaaugacgca      120

ggagaagcaa uuaguuuucc aacaacauua ggaaugaaua aauguuacau acaaauuaug      180

gaucuaggac acauguguga ugcaacaaug ucauaugaau guccgaugcu agacgaagga      240

guagagccag augauguuga cugcuggugc aauacaacaa gcacaugggu agucuacgga      300

acuugccauc auaagaaagg agaggcacga agaagccgga gagcuguaac uuuaccuagc      360

cauucuacuc gaaaauuaca aacaagauca caaacuuggc uagaaagucg agaauauaca      420

aaacauuuaa uaagaguaga aaacuggaua uuccguaauc cuggguuugc ucuugcagca      480

gcagcaauag cguggcuacu aggaaguagc acaagucaaa aaguaauaua cuuagugaug      540

auauuauuaa uagcuccagc auauucaauu agauguaucg gugugaguaa uagagauuuc      600

guagaaggaa ugaguggagg gacuugggua gauguaguuu uagaacacgg aggauguguc      660

acagugaugg cucaagacaa accaacugua gacauagaac uuguaacuac cacaguauca      720

aacauggcag aaguuagaag uuauuguuau gaagcaucua uaucagauau ggcaucagau      780

ucacgauguc caacacaagg cgaagcguau cuagauaaac aaucggauac ucaauaugua      840

uguaaaagaa cuuuaguaga uagaggaugg ggaaauggau guggccuauu cggaaaagga      900

ucauuaguga caugcgcuaa auuugccugu agcaagaaaa ugaccgguaa aaguauucaa      960

ccagaaaauu uagaauauag aauaauguua ucaguucacg gaucacaaca uaguggaaug     1020

auaguucaag auacaggcca cgaaacugac gaaaauagag caaaaguaga aauaacacca     1080

aauucuccaa gagcagaagc aacacuaggc ggauuuggau cauuaggauu ggauugugaa     1140

ccucgaacag gauuagacuu uagugauuug uauuauuuaa caaugaacaa caaacauugg     1200

uuaguacaca aagaaugguu ucacgauaua ccacuaccau ggcaugcagg agcagauacg     1260

ggaacacccc auuggaauaa uaaagaagcg cuuguagaau uuaaagaugc ucaugcaaaa     1320

aggcaaacag uaguaguauu aggaagucaa gaaggagcag uacauacagc ucuagcugga     1380

gcccuggaag cugaaaugga uggggcuaaa ggaagacuua gcucaggaca ucuaaagugc     1440

cgauuaaaga uggacaaauu aagauuaaaa ggcguaaguu auucuuuaug uacagcagca     1500

uuuacauuua caaaaauacc agcagaaacg cugcauggua cuguaacagu agaaguacaa     1560

uaugcaggaa cugacggacc auguaaagua ccagcacaaa uggcgguuga uaugcaaaca     1620

uuaacaccug uaggaagacu cauuacagca aacccaguua uaacagaauc uacagaaaau     1680

uccaagauga ugcuggaguu agauccccca uuuggugaua guuauauagu uaucggugua     1740

ggugagaaga aaauaacaca ucacuggcac cgaaguggau caacaauagg uaaagcauuc     1800

gaagcaacag uaagaggagc aaaaaggaug gcaguauuag gugacacagc cugggauuuu     1860

ggaucuguag gaggagcacu uaauucauua ggaaaaggca uucaccaaau auuuggagca     1920

gcauuuaaau cauuauucgg uggaauguca ugguuuucac aaauuuugau agguacacua     1980

cuaauguggc uaggacuaaa caccaaaaau gggucaauaa gucuaaugug cuuagcguua     2040

ggaggaguac uaauauuccu aaguacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4692
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4692
gggagaaagc uuaccaugac auccguaggu auaguagguu uacuauuaac uacugcgaug       60

gccgcagaag ucacaagaag agguucagcu uacuauaugu aucuagaucg uaaugacgca      120

ggagaagcaa uaucauuccc uacaacauua gguaugaaua aauguuauau ccaaauaaug      180

gauuuagggc auauguguga ugcaacaaug aguuaugaau gcccaaugcu agaugaagga      240

guagaaccag acgacguuga cugcuggugu aauacaacaa gcacaugggu aguauacgga      300

acaugucacc auaaaaaagg agaagcucga aggucacgac gagcgguuac auugccaucu      360

cauaguacaa gaaaacuaca gacaagauca cagacauggc uagaaaguag agaauacaca      420

aaacaucuaa uucgaguaga aaacuggaua uuuagaaauc caggcuuugc acucgcagca      480

gcugcaauag cauggcucuu gggaaguuca acaucacaga aaguaauaua ccuaguaaug      540

auauuacuua uagcaccagc cuauaguauu agguguauag gaguaucuaa uagagauuuu      600

guagaaggaa ugagcggagg gacaugggua gauauaguac uagaacaugg aggaugugua      660

acuguaaugg cacaagauaa accaacaguu gacauagaac uaguaacaac aacaguauca      720

aauauggcag aaguaagaag uuauuguuau gaagcgagua uaagugauau ggcaucagau      780

ucaagaugcc cuacacaagg agaagcauac cuagacaaac aaucggauac acaauaugua      840

uguaaaagga cauuaguuga uagagguugg gggaacggau guggguuauu cggaaaagga      900

aguuuaguaa ccugcgccaa auuugcaugu aguaagaaaa ugacagggaa aaguauccaa      960

ccugaaaauc uggaauacag aauuauguua agugugcaug gaagucaaca caguggaaug     1020

auaguaaaug auacgggaca cgaaacagau gaaaauaggg caaaagucga aauuacacca     1080

aacagcccca gagcagaagc aaccuuagga ggauuuggau cauuaggauu agauugcgaa     1140

ccaagaaccg gauuagacuu uagcgaucug uacuauuuga caaugaauaa uaaacauugg     1200

cuaguacaua aagaaugguu ccaugauaua ccauuaccau ggcacgcagg ugcugauacu     1260

ggcacaccac auuggaacaa caaagaagca cucguugaau ucaaagacgc ccaugcaaaa     1320

cgacaaacag uagucguuuu aggaucacaa gaaggagccg uacacacugc auuagcagga     1380

gcauuagaag cagaaaugga uggcgccaaa gggagacuau cgagugguca ucuuaagugc     1440

agacuaaaaa uggauaaauu aagguuaaaa ggaguaaguu acucucuaug cacagcagca     1500

uuuacauuua cuaaaauacc ugcugagaca uuacauggaa cuguaacugu agaaguacaa     1560

uacgcaggga cugauggacc auguaaagug ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccag uaggacgauu aauaacggca aauccaguaa ucacugaauc aacugaaaau     1680

aguaaaauga uguuagaguu agauccacca uucggcgaua guuauauagu aauaggagug     1740

ggcgaaaaaa aaaucacuca ucauuggcac agaagcggca guacauuagg aaaagcauuc     1800

agcacaacau uaaaaggagc ucaaagauua gcagcacuag gugauacagc augggauuuc     1860

ggaucaauug gaggaguauu caauucaaua gggagagcag ugcaucaagu auucgguggc     1920

gcauuuagaa cucuguucgg aggaaugagu uggauaacac aaggacuaau gggugccuua     1980

uuauuaugga ugggaguaaa ugcacgagac cgaucaauag cacuagcauu ucuagcaacu     2040

ggagguguau uaguauuucu ggcaacuaac guccacgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4693
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4693
gggagaaagc uuaccaugac caguguagga auaguagguu uacuccuuac cacagcaaug       60

gcggcagaag uaacacggag aggaucugca uauuacaugu accuugacag aaaugacgcg      120

ggagaagcaa uaaguuuccc aacaacacua gggaugaaua aaugcuacau ucaaauuaug      180

gaucuaggac auacauguga ugcgacgaug agcuaugaau gcccaauguu agaugaaggu      240

guagaaccag augauguaga cugcuggugu aauacuacau cgaccugggu uguauaugga      300

acaugucauc auaagaaagg agaagcacga agaagcagga gagcaguaac auuacccagu      360

cauucaacaa gaaaauuaca aaccagauca caaacauggc uagaaucaag agaauauaca      420

aaacauuuaa uaagaguaga aaacuggauu uuuagaaauc caggauuugc auuagcugcu      480

gcagcuauag cauggcuucu uggcaguuca accagccaaa aaguuauaua uuuggucaug      540

auuuugcuaa uagcaccagc auauaguaua cgauguauag gaguaucaaa uagagacuuu      600

guugaaggaa ugucuggagg aacauggguc gauguaguac uagaacaugg aggaugugua      660

acaguaaugg cacaagauaa accaacaguu gauauagaau uaguaacaac aacaguaucg      720

aauauggccg aaguaagaag uuauuguuau gaagcaucaa uauccgauau ggcaucagau      780

agcagauguc caacucaagg agaagcauac cuagauaaac aaucagauac ucaauauguu      840

uguaaaagaa cauuaguuga cagaggcugg gguaauggau gcgguuuauu cggcaaaggc      900

ucauuaguaa caugugcaaa guuugcaugu ucaaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaauu uagaauaucg aauaauguua aguguacaug gaucccagca cucaggaaug     1020

auaguuaaug auacagguca cgaaaccgau gagaauagag caaaaguaga gauaacucca     1080

aauaguccaa gagcagaagc uacauuagga ggauuuggua gcuuagguuu agauugugaa     1140

ccacgaacag gauuagauuu cagugaucua uauuaucuaa cuaugaauaa uaaacacugg     1200

uuaguacaua aagaaugguu ucaugauauc ccacuuccau ggcaugcagg agcagauaca     1260

ggaacacccc auuggaauaa uaaagaagca uuggucgaau uuaaagaugc acaugcaaaa     1320

aggcaaacag uuguaguguu aggaucacaa gaaggagcug uacacacagc acuugcuggu     1380

gcacuugagg cagaaaugga uggagcaaaa ggaagacuga gcagcgggca uuuaaaaugu     1440

agauugaaaa uggauaaauu acgccugaaa ggaguaaguu auagucuaug uaccgcagca     1500

uuuacauuua caaaaauacc agcugaaacg cuacauggaa cggucacagu agaaguacaa     1560

uaugcaggua cagauggacc auguaaagua ccagcacaaa uggcaguaga caugcaaaca     1620

cuaacaccag uuggaagauu gaucacugca aauccaguua uaacagaauc aacugaaaau     1680

ucgaaaauga uguuagaguu agacccuccg uuuggugacu cauauauagu aaucggagua     1740

ggagaaaaga aaauaacaca ucacuggcau agaucaggaa guacacuagg aaaggcuuuu     1800

agcacaacau uaaaaggugc ucaaagauua gcagcauuag gcgauacagc uugggacuuu     1860

ggaucaauag gcggaguauu uaacaguaua ggaagggccg uacaucaagu auuuggagga     1920

gcauuuagaa cacucuuugg aggaaugagc uggauaacac aagguuuaau gggagcauua     1980

uuacuuugga ugggaguaaa ugcacgagau cgaaguauag cauuagcauu ucuagcaacu     2040

gguggagucc uaguauuucu agcaacaaac guacaugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4694
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 1 opt

<400>  4694
gggagaaagc uuaccaugac aucaauaggu aucauaggcc uauuacuaac uacagcaaug       60

gcagcagaaa uaacuagaag agguucagca uauuacaugu aucuagauag aagugaugca      120

gguaaagcaa uaaguuuugc aacaacauua ggaguaaaua aaugccaugu acaaauaaug      180

gaucuagguc auauguguga cgcaacaaug aguuaugaau gcccuaugcu agacgaagga      240

guugaaccug augacguaga uuguuggugc aacacaacaa guacuugggu gguauaugga      300

acuugucauc acaagaaagg agaagcaaga cgaucaagac gagccguaac ucuaccaucu      360

cacucaacaa gaaaacuaca aacacgcuca cagacauggu uagaaucaag agaauacacu      420

aaacauuuaa uuaaaguuga aaacuggauu uuucgaaauc cagguuuugc auuaguagcu      480

guagccauag cauggcuacu aggaaguucu acaucucaaa aaguaauaua ucuuguaaug      540

auacuacuaa uagcuccagc auauucaauu agauguauag gaguaagcaa uagagauuuc      600

guagaaggaa ugaguggagg aacaugggua gauguaguau uagaacaugg ugguugugua      660

acaguaaugg cacaagacaa accaacaguc gacauagaau uaguuacaac uacgguaagu      720

aacauggcag aaguuaggag uuauuguuau gaggcaucaa uaagcgauau ggcgucagau      780

ucaagaugcc caacacaagg agaagcauau uuagacaaac aaagugauac gcaauacgua      840

uguaaaagaa cucuaguaga cagggguugg ggaaauggau gcgguuuauu uggaaaaggc      900

agucuaguaa caugcgcaaa auuuacaugu ucaaagaaaa ugacaggaaa aucuauucaa      960

ccugagaauu uagaauaucg aauaauguua ucuguacaug gaucucaaca uucaggaaug     1020

auaggguaug aaacugacga agauagagca aaaguagaag uaacgcccaa uucuccaaga     1080

gcagaagcaa ccuuaggcgg auuuggaagu uuaggacuag auugugaacc aagaacaggu     1140

cuagacuuuu cagaucucua cuaucuaaca augaauaaua aacacuggcu ugugcauaag     1200

gaaugguuuc augauauccc auuaccaugg caugcaggag cagacacagg aacaccucau     1260

uggaauaaua aagaggccuu aguagaauuu aaagaugcac augcgaaaag acaaacagua     1320

guaguuuuag gaagucaaga gggagcaguu cauacagcau uagccggagc acuugaagcg     1380

gaaauggaug gagccaaagg acgucuauuu aguggacacu uaaaaugcag auuaaaaaug     1440

gauaaacuua gacuaaaagg ugucucauac ucucuaugca cagccgcguu uacauuuacc     1500

aagguaccag ccgaaacacu ucacggaacc guaacuguag aaguacaaua ugcaggaaca     1560

gauggaccau guaaaauucc uguacaaaug gcaguagaua ugcaaacauu aacacccgua     1620

gguagauuaa uaacagcaaa ucccguaauu acagaaucaa cagaaaauag uaaaaugaug     1680

uuagaacuag auccuccauu uggagauuca uauauaguaa uaggaguagg ugacaagaaa     1740

auaacacauc auuggcacag aagugguagc acauuaggaa aagcauuuag uacaacacuc     1800

aaaggagcac aaagauuagc agcacuagga gauacagcau gggacuuugg aagcauugga     1860

gguguauuua auaguaucgg uagagcaguu caucaagugu uuggaggagc auucagaaca     1920

cuauuuggag guaugucuug gauuacacaa gguuuaaugg gugcauuauu auuguggaug     1980

ggaguaaaug cacgagaucg uucaauagca uuggccuuuu uagcaaccgg uggaguccug     2040

guguuuuuag caacuaaugu ccaugcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4695
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4695
gggagaaagc uuaccaugac aucuguaggu auaguaggac uccuuuuaac aacagcaaug       60

gcagcagaag uaacgagaag agguagcgca uauuauaugu auuuagauag aaacgacgcc      120

ggagaagcaa uuagcuuucc uacaacauua ggcaugaaua aauguuauau acaaauaaug      180

gaccuuggac auauguguga ugcaacuaug agcuaugaau gcccuaugcu agaugaggga      240

guagaaccag augacguaga uuguuggugu aacacaacaa gcacaugggu cguuuauggc      300

acaugccauc auaaaaaagg agaggcaaga cguagucgaa gagcaguaac acuaccaucg      360

cauucaacac gaaaauuaca aacuagaagc caaacauggc uagaaucacg agaguacaca      420

aaacauuuaa uaagaguaga aaacuggauc uuuaggaauc cugguuuugc acuagcagca      480

gcagcaaucg cauggcuacu cggaaguagu acgagucaaa aaguaaucua ucuaguaaug      540

auuuuacuaa uagcacccgc uuacucaaua agguguauag guguaucaaa uagagauuuu      600

guggaaggua ugaguggugg aacauggguu gauguaguac uagaacaugg aggauguguc      660

acaguuaugg cucaagauaa accaaccguu gauauagagu uaguuacuac uaccguuucc      720

aauauggcag aaguaagaag cuacugcuau gaagcaagca ucucagauau ggcaucagau      780

ucaagaugcc caacccaggg agaagcauau uuagacaaac aaucagauac acaauaugua      840

ugcaaaagga cauugguuga uagaggaugg gguaauggau gcggauuauu uggcaaagga      900

ucucuaguaa ccugugcuaa auucgcaugc ucuaaaaaaa ugacaggaaa aucgauucaa      960

ccggaaaauc uugaauaccg aauaaugcuu ucaguacaug gaagccaaca uucaggaaug     1020

aucguaaacg auacagguca cgaaacagau gagaauagag cuaaaguaga aauaacucca     1080

aauagcccac gugcugaagc uaccuuagga ggauuugggu cuuuaggauu agauugcgaa     1140

ccacgaacag gauuagauuu cucagaccuc uauuaucuca caaugaacaa uaaacauugg     1200

uuaguccaua aagaaugguu ccaugacauc ccacuaccau ggcacgcagg ugcggacacu     1260

ggaacaccgc acuggaacaa caaagaagca cuagucgaau ucaaggaugc ucacgcaaaa     1320

agacaaacag uaguaguacu gggaucacaa gaaggagcag uacacacagc uuuagcagga     1380

gcauuagaag cagaaaugga cggagcaaaa ggaagauuaa gcagcggaca ucuuaaaugu     1440

agauuaaaaa uggauaaauu gagauuaaaa ggagucucgu auucauuaug cacagccgca     1500

uuuacauuua caaaaauacc cgccgaaaca cuacauggaa caguuacugu cgaaguacaa     1560

uaugcaggga cagacggucc auguaaagua ccugcacaaa uggcaguuga caugcaaaca     1620

cuaacaccag uaggaagauu aaucacagca aacccaguaa uaacagaauc uacagaaaac     1680

aguaaaauga uguuagaauu agauccaccu uuuggcgauu ccuauauagu aauuggagua     1740

ggagaaaaaa aaauuacaca ucauuggcau agaagugguu caacacuagg caaagcuuuu     1800

ucaaccacac uaaaaggugc ucagcgacua gcagcacuug gagauacagc augggauuuc     1860

ggaaguaucg gaggagucuu uaacucaaua ggucgagcag uucaucaagu auucggcgga     1920

gcauuuagaa cacuauuugg uggaauguca uggauaacac aaggacuaau gggagccuua     1980

uuauuaugga ugggaguaaa ugcgagagau agaaguauug cauuagcauu uuuagcaacu     2040

gguggaguac uaguauuuuu agcaacuaac guacaugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4696
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  4696
gggagaaagc uuaccaugag aauuauaaac gcucgaaaag aaaaaaaaag acgaggagca       60

gauacaagug uugguauagu agguuuauug uuaacuacug caauggcagc agaaguaaca      120

agacgaggaa gcgcuuauua uauguaucua gacagaaaug augcaggaga agcgauaagu      180

uucccaacua cauuagguau gaauaaaugu uacauacaaa uaauggauuu agggcauaug      240

ugugaugcaa caaugaguua ugaaugccca auguuagaug agggaguaga acccgaugac      300

guagauugcu ggugcaacac uacaagcaca uggguaguau augguacaug ucaucauaaa      360

aaaggcgaag caagaagaag ccgaagagca guuacacuac caucacauuc aacaagaaaa      420

cuucaaacgc gaucacagac uuggcuagaa ucaagagaau auacaaaaca ucuaauuagg      480

guagaaaauu ggaucuucag aaacccagga uuugcgcuag cagcugcugc uauugcuugg      540

uuauuaggau caucaacaag ccaaaaagua auuuaucuug ugaugauucu acuaauugca      600

cccgcauauu cuauacgcug cauaggagug ucgaaccgag auuuugucga aggaaugucc      660

gggggaaccu ggguagauau cguauuagaa cauggagguu guguuacugu gauggcacaa      720

gauaaaccaa caguagauau agaauuaguu acaacaacag uaagcaauau ggcagaaguu      780

agauccuacu guuaugaagc auccauuagc gauauggcua gugauagcag auguccaaca      840

caaggagaag cuuacuuaga uaaacaaagu gacacacaau auguaugcaa aaggacauug      900

guagaccgag gauggggcaa uggaugcggu uuauucggua aaggcucauu aguaacuugu      960

gcaaaauuug cguguucaaa aaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa     1020

uacagaauaa uguuaucagu acacggaucc caacacucag gaaugauagu aaaugacaca     1080

ggacaugaaa cagaugaaaa cagagcaaaa guagagauaa caccaaauuc accaagagcu     1140

gaagccacau uaggaggauu cggaaguuua gguuuagacu gugaaccaag aacuggauua     1200

gauuuuagcg acuuauauua uuuaacaaug aacaauaaac auugguuagu acauaaagaa     1260

ugguuucaug auauaccauu acccuggcau gcaggagcug auacagggac uccccauugg     1320

aacaauaaag aagcacuugu agaauucaag gaugcacaug cgaaaagaca aacaguagua     1380

gucuuaggca gccaagaagg ggcaguucac acagcauuag caggagcuuu agaagcggag     1440

auggauggcg caaaaggaag auuaagcuca ggccacuuaa aaugcagguu aaagauggac     1500

aaauuaagau uaaaaggagu aaguuauagc cuaugcacag cagcauuuac auucacuaaa     1560

auaccagccg aaacauuaca cggaaccguu acuguugaag uacaauacgc aggaacagac     1620

ggacccugca aaguaccagc ucaaauggcc guagauaugc aaacauuaac ucccguuggg     1680

agacuaauaa cagccaaucc aguaauaaca gaaaguacug aaaauucaaa aaugauguua     1740

gaauuagauc caccuuuugg agacucauac auaguuauag guguagggga aaagaaaaua     1800

acacaccacu ggcacagauc aggcucaacu auugguaaag cauuugaagc uacagucaga     1860

ggcgcgaaaa gaauggcagu acuaggagau acagcauggg auuucggauc aguuggaggg     1920

gcauuaaaca guuuaggaaa aggaauacau caaaucuucg gagcagcuuu caaaucuuua     1980

uuuggaggua ugucuugguu uucucagaua cuaauaggua cauuauuaau gugguuagga     2040

cuaaauacaa aaaauggguc aaucucuuug auguguuuag cacuaggugg aguguugaua     2100

uuuuuaucaa cagcaguaag ugcagacgua gguuguucag uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4697
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4697
gggagaaagc uuaccaugau aauuaacgcu aggaaagaaa aaaaacgaag aggggcagau       60

acauccguag gcauaguagg uuuguuauua acaacagcca uggcagcaga aguaacacga      120

agaggcucag cauacuacau guauuuagau agaaaugaug caggagaagc aauaagcuuu      180

ccaacuacau uaggaaugaa uaaauguuau auacaaauca uggaucuagg acacaugugu      240

gaugcgacaa ugucauauga augcccaaug uuggaugaag gaguugaacc agaugauguc      300

gauugcuggu guaauacaac aaguacaugg guaguauaug gaacaugcca ucauaaaaaa      360

ggagaagcca gaagaagcag acgcgcagua acacucccau cucauaguac aagaaaacua      420

caaacaagau cucaaaccug gcuagagagc agagaauaca caaaacauuu aauaagagua      480

gagaauugga uuuucagaaa uccuggauuu gcccuggcag cagcagcuau ugcauggcua      540

cuugguucga gcacgagcca aaaaguaauc uaucucguua ugauacuacu aauagcucca      600

gcuuacagca uaagauguau uggaguauca aauagagauu uuguagaagg uaugucagga      660

ggaacauggg uagauaucgu uuuagaacau ggaggaugug uaacaguaau ggcgcaagac      720

aaaccaacgg uagacaucga auuaguaaca acgacaguaa guaauauggc agaaguaaga      780

aguuacugcu acgaagcaag uauuucagau auggcaucag acucacgaug uccaacacaa      840

ggagaagcau auuuggauaa acaaucagac acacaauaug uaugcaaaag aacacuagua      900

gacagaggau gggggaacgg auguggacuu uuuggaaaag guucuuuagu aacaugugca      960

aaauuugcgu gcucaaaaaa gaugacagga aaaaguauac aaccagaaaa ucuugaauau     1020

agaauaaugu uaucaguaca cgggagccag cauagcggca ugauaguuaa cgacacugga     1080

cacgaaacag acgaaaacag agcuaaagua gaaauaaccc caaauagucc cagggcagaa     1140

gcaacguuag gaggauuugg aaguuuagga cuagacugug aaccacgaac aggacuagau     1200

uuuagcgauc uauacuacuu aacaaugaac aacaaacacu ggcuagucca uaaagaaugg     1260

uuucaugaca uaccguuacc auggcaugca ggagccgaca ccggaacucc acauuggaau     1320

aacaaagagg cauuaguaga auuuaaagau gcacaugcaa aaagacagac uguaguagua     1380

cuaggaaguc aagaaggagc uguacacaca gcacuugcag gagcauuaga agcagaaaug     1440

gacggagcaa aagggcgguu gucauccgga caucuaaaau gucgauuaaa aauggacaaa     1500

uuaaggcuua aaggaguauc uuacagucua ugcacagcag cauuuacauu uacaaaaaua     1560

ccagcagaaa cauuacaugg uacaguaaca guugaaguac aauaugcagg aacagauggc     1620

ccauguaaag uuccagcaca gauggcagua gauaugcaaa cuuuaacccc aguaggaaga     1680

uugauaacag cuaaucccgu aauaacagaa aguacagaaa auucaaaaau gauguuagaa     1740

cuggauccac cauuuggaga uagcuauaua guaauaggag uaggagagaa aaagauaaca     1800

caucauuggc acagaagugg aagcacaauc ggaaaagcuu uugaagcaac gguaaggggc     1860

gcaaaaagaa uggcaguauu aggagacacc gcgugggauu uuggaagcgu aggaggagca     1920

uuaaauaguu uaggaaaagg aauacaucaa aucuucggag cugcauucaa aucauuauuu     1980

ggugguaugu caugguuuuc acaaauacua auagguaccc uucuaaugug guuaggauua     2040

aauaccaaaa acggcagcau aagcuuaaug uguuuagcau uggguggugu uuuaauuuuu     2100

uuaucuacag caguuucagc agaugucggu uguagcgugg auuuuagcaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  4698
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4698
gggagaaagc uuaccaugau aaaugcaaga aaagaaaaaa aacgaagagg agcagacaca       60

agugucggaa uaguagguuu auuauuaacu accgcaaugg cggcagaagu aacgagaaga      120

ggaucugcau auuacaugua uuuagaucgu aaugaugcug gagaagcaau aucuuuccca      180

acaacacuag gaaugaauaa augcuauauu caaauaaugg accuaggaca uaugugcgau      240

gcaacaauga guuaugaaug uccaauguua gacgaaggag uagaaccaga cgauguggac      300

uguuggugua auacaacuag cacgugggua gucuacggaa caugccauca uaaaaaaggg      360

gaagcaagaa gaaguagacg agcaguaacg uuaccaucac auagcaccag aaaauuacaa      420

accaggucac aaacuuggcu agaaucacga gaauacacaa aacaucuaau uagaguagaa      480

aacuggauau uucgaaaccc gggauuugcc uuagcugcag cagcaauagc auggcuauua      540

ggaucaagua cuucacaaaa aguaauuuau cuaguaauga uacuauuaau agcgccagca      600

uauucaauaa gguguauugg aguuagcaau agagauuuug uagaaggaau gaguggagga      660

acuugggucg auaucguacu agaacaugga ggauguguaa caguaauggc acaagacaaa      720

ccaacaguag auauagaacu aguuacaaca acaguaagua auauggccga agugagaagc      780

uauuguuaug aagccucaau aucagauaug gcaagugacu caaggugucc aacucaagga      840

gaagcauauc uggauaagca aucagacaca caauaugucu guaaaagaac guuaguagau      900

agagguuggg gaaauggaug uggucucuuc ggaaaaggau cauuaguaac augugcaaaa      960

uuugccugca gcaaaaaaau gacaggaaaa agcauacaac cugaaaaccu ggaauauaga     1020

auaaugcucu caguacaugg uagccaacau ucagguauga uagugaauga uacaggacau     1080

gaaacugaug aaaaucgagc aaaaguagaa aucacgccua auaguccgag agcugaagcu     1140

acacuaggag gauuuggaag uuuaggauua gacugugaac caagaacagg auuagauuuu     1200

agugaccucu acuauuuaac aaugaauaau aaacauuggc ucguacauaa agagugguuu     1260

cacgacauac cauuaccaug gcacgcaggu gccgauacag gaacuccgca uuggaauaac     1320

aaagaagcau ugguugaauu uaaagaugca caugcaaaga gacaaacagu aguuguuuua     1380

ggaagccaag aaggagcagu ccacacagcu uuagcaggag cuuuagaggc agaaauggau     1440

ggagcaaaag gaagacuuag cucuggccac cuaaaaugca gauugaaaau ggauaaacug     1500

agacuaaaag gaguaucuua uagucuaugu acagccgcau ucacauuuac uaaaauucca     1560

gcagaaacuu uacaugguac aguaacugua gaaguucaau augcaggaac agauggcccu     1620

uguaaaguac cugcccaaau ggccguagau augcaaacau uaacaccagu aggaagauua     1680

auaacagcaa auccaguaau aacagaaagc accgaaaaua gcaaaaugau guuagaauua     1740

gauccaccau uuggugauag uuauauagua auuggaguag gcgaaaaaaa aauaacacau     1800

cacuggcaua gaucaggguc aacaauaggu aaagcauuug aagccacagu aagaggagca     1860

aaaagaaugg caguuuuagg agauaccgcc ugggacuuug guucaguagg aggagcucuu     1920

aauucuuuag gaaaaggaau acaccaaaua uuuggcgcag cauuuaagag uuuauucgga     1980

ggaaugucau gguuuucaca aauucuaaua ggaacguuau uaauguggcu agguuuaaau     2040

acaaaaaaug ggagcaucuc ccuaaugugu cuugcauuag gaggaguacu uauauuuuua     2100

agcacugcag uaagugcgga cguagguugu aguguagauu uuagcaaaug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  4699
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4699
gggagaaagc uuaccaugaa cgcuagaaaa gaaaaaaaac guagaggugc cgacacaagc       60

guaggaauug uaggguuauu auuaacaaca gcaauggcag cugaaguaac uagaagaggu      120

ucagcauacu auauguacuu agauagaaau gacgcuggag aagcaauaag uuuuccaaca      180

acauuaggua ugaauaaaug uuauauacaa auaauggauc uuggucauau gugcgaugcc      240

acaaugucgu acgaaugucc aaugcuagau gaaggagucg aaccagauga uguagauugu      300

ugguguaaca caacaagcac uuggguagug uaugguacau gucaucacaa aaaaggagag      360

gcaagaagaa guagacgggc uguuacacuu cccagccacu cuacgagaaa acuacaaaca      420

cgaagucaaa cauggcuaga aucaagagaa uacacaaaac accuaauaag aguagaaaau      480

uggauauuua gaaauccagg guucgcucuu gcagcagcag caauagcaug gcuauuagga      540

aguaguacua gucaaaaagu gauauaucua guaaugauac uuuuaauagc gccagccuac      600

agcauaagau guaucggagu aaguaauaga gauuuugucg aaggaauguc uggagguacu      660

uggguagaca uaguacuaga acacggugga ugcgugacug uaauggcaca ggacaaaccc      720

acaguagaca uugaauuagu aacaacaaca guaucaaaca uggcagaagu aagaucauau      780

uguuaugaag caaguauaag ugauauggca ucagauaguc gauguccaac acaaggagaa      840

gcuuauuuag auaaacaaag ugauacgcaa uacguaugua aaagaacgcu aguagacagg      900

gguuggggaa acggaugugg uuuauucggu aaggguucau uaguaacaug ugccaaauuu      960

gcauguagua aaaaaaugac uggaaaaucu auacaaccug aaaauuuaga auacagaauu     1020

auguuaucgg uucauggaag ccagcacagc ggaaugauag uuaaugauac aggacaugaa     1080

acggaugaaa auagagcaaa aguagaaaua acaccaaaca guccaagagc agaagcaaca     1140

uuaggaggau uuggaagcuu aggauuggau ugugagccga gaacaggauu agauuuuagc     1200

gaucuauacu auuuaacaau gaauaacaaa cauugguuag uacauaaaga augguuucac     1260

gauauuccac uaccguggca ugcaggagca gauacaggua caccgcauug gaauaacaaa     1320

gaggccuuag uugaguucaa agaugcucau gcuaaaagac aaacggucgu aguacuagga     1380

ucacaagaag gagcaguaca cacagcacua gcaggagcuc uugaagccga aauggauggu     1440

gcgaaaggac gauuaucaag uggacauuua aaaugcagac uaaaaaugga uaaauugaga     1500

uuaaaaggag uaaguuacag cuuauguaca gcugccuuua cuuucacaaa aaucccagcu     1560

gaaacauuac auggaacagu uacaguugaa guucaauaug caggaacaga ugguccaugu     1620

aaaguaccag cacaaauggc aguagauaug caaacauuaa cuccaguagg aagauuaaua     1680

acugccaauc cuguaaucac agaaaguacc gaaaauucaa aaaugauguu agaauuggau     1740

ccgccauuug gagauucuua uauagucaua gguguaggug aaaaaaaaau aacacaucau     1800

uggcauagaa gugguucaac aauagguaaa gcauucgaag caacaguaag gggagcaaaa     1860

agaauggcag uacuaggaga uacagcuugg gacuuuggua gcgucggagg ugcuuuaaau     1920

agcuuaggaa aagguauuca ucaaauauuu ggugccgcau uuaaaaguuu auucggaggg     1980

augaguuggu uuagucaaau acucauugga acucuuuuga uguggcuagg auuaaacaca     2040

aaaaauggaa guauuucacu aauguguuua gcauuaggag gaguauuaau auuccuaucu     2100

acagcagucu cagcugaugu aggaugcuca gucgacuuua guaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4700
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4700
gggagaaagc uuaccauggc aagaaaagaa aagaaaagga gaggagcaga cacaagugua       60

ggaauaguag guuuauuacu gacuacggca auggcagcag aaguuacaag aaggggaagc      120

gcauauuaua uguauuuaga uagaaaugau gcaggagaag caauaucauu cccaaccaca      180

uuaggaauga acaaauguua cauacaaaua auggaccucg gacauaugug cgaugccaca      240

augucauaug aaugcccgau guuagacgaa ggaguagaac cagaugaugu agauuguugg      300

uguaauacua cuagcacaug gguaguauac ggaacaugcc aucauaagaa aggagaagcu      360

cgaagaucua gaagggcugu aacuuuaccc ucacauucaa cacguaaauu acagacaaga      420

agccaaacau ggcuagaauc aagggaauau acaaaacacu uaauuagagu agaaaauugg      480

auauucagaa auccaggauu cgcguuagca gccgcagcga uagcuugguu auuaggauca      540

aguacaucuc aaaaaguaau auaucuaguc augauacuac uaauagcgcc agcuuacagu      600

auaagaugca uaggaguaag uaacagagac uuuguagaag gaaugucagg aggcacaugg      660

guagacauag uacuagaaca ugguggaugu guaacaguga uggcacaaga caaaccaaca      720

guagauauag aauuaguaac aacgacagua agcaauaugg cagaaguaag gaguuauugu      780

uaugaagcau caauuucaga uauggcaagc gauucaagau gucccacuca aggugaagca      840

uaucuagaua aacaaagcga uacgcaauau guauguaaaa gaacuuuagu agaucgaggc      900

ugggguaacg gaugcgguuu auuuggaaaa gggagcuuag ucacaugugc aaaauucgca      960

uguucgaaga aaaugacugg aaaaaguaua caaccugaaa aucuagaaua cagaauuaug     1020

uuaucaguac auggaaguca acauagugga augauuguaa acgauacagg ucaugaaacg     1080

gacgaaaauc gcgcaaaagu agaaauaaca ccuaauaguc cacgagcaga agcuacuuua     1140

ggggguuucg ggucauuagg auuggacugu gaaccaagaa cagguuuaga cuuuagugac     1200

cuauauuacu uaacaaugaa caauaaacac ugguugguac acaaagaaug guuucaugac     1260

auaccauuac ccuggcaugc aggagcagac acuggaacuc cacauuggaa uaauaaagaa     1320

gcgcuaguag aauuuaaaga ugcacaugca aaaagacaaa cuguaguugu ccuaggauca     1380

caagaaggag caguacauac agcauuagcu ggagcauuag aggcagaaau ggauggagca     1440

aaaggaagau uauccagugg gcaucuaaaa ugcagguuaa aaauggauaa acuuaggcua     1500

aaaggaguau cuuacucacu auguaccgcu gcauucacau uuacaaaaau uccugcagaa     1560

acauuacaug gaacagugac aguagaagug caauaugcug gaacugacgg uccauguaaa     1620

guuccagcac aaauggcagu agauaugcaa acauuaacuc cuguagguag acuuauaaca     1680

gcaaacccag uaauaacaga aaguacagaa aauucaaaaa ugaugcuaga auuagaucca     1740

ccauucggag auagcuauau agugauugga guaggugaaa agaaaauuac acaccacugg     1800

cacaggagug guaguacaau aggaaaagcg uuugaagcaa cuguaagagg ugcaaaaaga     1860

auggcugucu uaggugacac ugcaugggau uuugguagug uuggaggagc uuuaaauuca     1920

cuaggaaaag guauacacca aauauucgga gcagcauuua aaagucuauu uggcgguaug     1980

uccugguuuu cacaaauauu aauaggaacg uuauuaaugu gguuaggacu aaacacgaaa     2040

aauggaagca uaucauugau guguuuagcu cuaggaggag uuuuaauauu ucuuucaaca     2100

gcagucucag cagauguugg auguucagua gacuuuagua aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4701
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4701
gggagaaagc uuaccaugag aaaagaaaaa aaaagacgag gagcagacac aucagucgga       60

auuguaggac uacuuuuaac aacagcaaug gcagcagagg uuacuaggag agguucagcc      120

uauuauaugu auuuagauag aaacgaugcu ggcgaagcaa uuaguuuccc cacaacauua      180

ggaaugaaua aauguuauau acaaauuaug gauuuggguc auaugugcga cgcaacaaug      240

aguuaugaau gccccaugcu ggaugaaggg gucgaaccag acgauguaga uuguuggugu      300

aacacaacau caaccugggu aguauacggg acaugucauc acaaaaaagg agaagcgaga      360

cgaagcagaa gagcaguuac acuaccaagu cacucaacac gaaaacuaca aacacgauca      420

caaacauggu uagaaaguag agaguacaca aaacaucuua uucgaguaga gaauuggaua      480

uuuaggaauc caggauuugc auuagcagca gcagcaauag caugguuauu agguucauca      540

acaagucaaa aaguaauaua ccuuguaaug auacuacuua uagcaccugc uuauaguaua      600

agauguauug gaguaucaaa uagagacuuu gucgaaggaa ugaguggagg cacgugggua      660

gauauaguau uagaacacgg aggaugugua acagucaugg cacaagacaa accuacagua      720

gacaucgaac ucgucacuac cacaguauca aauauggcag aaguaagauc auacuguuau      780

gaagccagua uaagugauau ggcaagugac ucaagauguc caacacaagg ugaagcauau      840

cucgauaaac aauccgauac ucaauaugua uguaaaagaa cauuaguaga uagaggaugg      900

ggcaauggau guggacuuuu uggaaaaggg ucacuaguaa caugcgcaaa auuugcaugu      960

aguaaaaaaa ugacagguaa aucaauacaa ccagaaaacc uugaauaucg aauaauguua     1020

aguguucaug gaagccaaca cucgggcaug aucgugaacg acacagggca cgaaaccgau     1080

gagaauagag caaagguaga aauaacacca aauagcccaa gagcagaagc uaccuuagga     1140

ggauucggau cauuaggcuu agauugugaa ccaagaacag gguuagacuu uucagaucua     1200

uacuaucuaa ccaugaauaa uaaacauugg cuaguucaca aagaaugguu ucaugauaua     1260

ccauuaccau ggcacgcagg agcagacacg ggaacaccac auuggaacaa caaagaagca     1320

uuaguggaau uuaaagaugc acacgcaaaa agacaaacug uugugguacu aggaagucag     1380

gaaggagcag uacauacagc auuagcggga gcgcuagaag cagaaaugga uggagcaaaa     1440

ggaagacuau caucugguca cuuaaaaugu agauugaaaa uggauaaguu aagauuaaag     1500

gggguuagcu auucucuaug uacagcggcc uucacuuuua caaaaauucc ggcagaaaca     1560

cugcauggaa caguaacagu agaaguccag uaugcaggua cagacggacc auguaaagua     1620

cccgcccaaa uggcaguaga uaugcaaaca uugacuccag uaggaagacu cauaaccgcu     1680

aauccgguua uaacagagag uacagaaaac ucaaaaauga ugcuagaacu cgauccgcca     1740

uucggagauu cguauauagu uauagguguu ggagaaaaaa aaauaacaca ccauuggcau     1800

agaucaggcu ccacaauagg aaaagccuuu gaagcuaccg uaaggggagc aaaaagaaug     1860

gcaguauuag gagauacugc augggauuuc ggaucaguug gaggagcacu aaauagcuua     1920

ggaaaaggaa uacaucaaau auuuggagca gcauuuaaau cuuuguuugg aggaauguca     1980

ugguucaguc aaauauuaau aggaacguua uuaauguggu uaggucugaa uaccaaaaau     2040

ggaagcauua guuuaaugug uuuagcauua ggaggaguau uaauauuucu aucgacagcu     2100

guaucagcag auguggguug uucaguagau uuuuccaaau gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  4702
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4702
gggagaaagc uuaccaugaa agaaaaaaaa agaagaggag cagauacauc aguaggaauc       60

guaggauuac uauugacaac agcuauggca gcagaaguaa caagaagagg aucggcauau      120

uauauguauu uagaucgcaa cgaugcugga gaagcaauuu cauucccaac cacuuuggga      180

augaauaaau gcuacauaca aauaauggau uugggacaua ugugugacgc gacuauguca      240

uacgaguguc cgaugcuaga ugaaggagua gaaccugaug acguagauug uugguguaau      300

acuacaagca cuuggguagu cuaugguacg ugccaucaua aaaaagguga agcucgaaga      360

agcagaagag caguuacuuu accuucacac ucaacaagaa aauuacaaac gagaagucaa      420

acaugguuag aaucaagaga auauacaaaa cauuuaauaa gaguugaaaa uuggauauuu      480

agaaauccug guuuugcauu agcugcggca gcaauagccu gguuauuagg aucaucaacu      540

agucaaaaag uaauuuauuu aguaaugauu uuauuaauag caccugcgua uaguauaaga      600

uguauaggag uaaguaauag agauuuugua gaagguaugu caggaggaac auggguagac      660

auaguauugg aacauggggg gugcguaaca gucauggcuc aagacaaacc aacaguagac      720

auagaauuag uuacaacaac uguauccaau auggcagaag uaagaagcua uuguuacgaa      780

gcuucuauaa gcgacauggc uucagauuca agauguccua cacaagguga agcauauuua      840

gacaaacaaa gugauaccca auacguaugc aaaagaacau ugguggauag aggauggggu      900

aauggaugcg gauuauuugg aaagggauca cuaguaacau gugcaaaauu ugcauguagc      960

aaaaaaauga caggaaaaag uauacaaccg gaaaaucuag aauauagaau aauguuaucg     1020

guacacggua gucaacauuc aggaaugaua guaaaugaua caggacauga aacagaugag     1080

aacagagcua aaguugaaau uacaccgaau ucuccaagag cagaagcaac gcuaggagga     1140

uuuggaaguc uagguuuaga uugcgaacca agaacagguu uagacuucag ugacuuauac     1200

uacuuaacaa ugaacaauaa acauugguua guacacaaag aaugguuuca ugauauacca     1260

cuaccauggc augcaggagc ggauacugga acaccacacu ggaacaauaa agaagcauua     1320

guagaauuua aagaugcaca ugcaaaaaga caaacaguag uagugcucgg uucacaagaa     1380

ggagcagugc acacagcauu ggccggagca cuagaagcag aaauggaugg agcaaaagga     1440

agacuaucau caggacaucu aaaaugcagg cuaaaaaugg auaagcuuag auuaaaagga     1500

guaucauauu cacugugcac agcagcuuuc acauucacca aaauuccugc agaaaccuug     1560

cacgguaccg uaacaguaga aguacaguau gccgggacag auggaccaug uaaaguacca     1620

gcgcaaaugg cggucgauau gcagacucua acaccagugg gaagguuaau aacagcuaau     1680

cccgugauaa cagaaucaac agaaaauagu aaaaugaugu uagaacuaga uccuccauuu     1740

ggagauucau acauaguaau aggaguugga gaaaaaaaaa uaacacauca cuggcauagg     1800

agcggcucaa ccauuggaaa agcauucgaa gcuacaguaa gaggcgcaaa acguauggca     1860

guauuaggag auacagcaug ggauuuugga ucaguuggag gugcauuaaa cucacucgga     1920

aaaggaauac aucaaauauu cggagcugcu uucaaauccu uguucggagg uaugaguugg     1980

uuuucucaaa uauuaauagg aacacuauug augugguuag gauuaaauac aaaaaacgga     2040

agcauaucau uaauguguuu agcauuaggg ggugucuuaa uuuucuuauc aacagcagug     2100

ucagcagaug ucggguguag cguugauuuu aguaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4703
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4703
gggagaaagc uuaccaugga aaagaaaaga agaggagcag auacaucagu gggaauugua       60

gggcuauuau uaacaaccgc aauggcagca gaaguaacaa gaaggggaag cgcauacuau      120

auguauuuag accguaauga cgcuggagaa gcgauuuccu uucccacaac auuaggaaug      180

aauaaaugcu acauacaaau aauggacuua ggacauaugu gugaugccac aaugucauac      240

gaauguccaa ugcuugacga aggaguagaa ccagaugacg ucgacuguug gugcaauaca      300

acaucuacuu ggguuguuua ugggacuugu caucacaaga aaggagaggc cagacgaagu      360

cguagagcag uaacuuuacc aagccacuca acaagaaaac uacagacuag aagucaaacg      420

ugguuagaau caagagaaua uacaaaacau uuaauacgag uggaaaacug gauauucaga      480

aacccuggau ucgcauuggc agcagcugca auugcauggu uauuaggauc aaguacauca      540

caaaaaguaa uauauuuagu uaugauauua cuaauugcac ccgcuuauuc aauaagaugu      600

auaggaguau cuaacagaga uuuuguugaa ggcaugagcg gaggaacaug gguagauaua      660

guauuggaac augggggaug cguuacagua auggcacaag auaaaccaac aguggauaua      720

gaauuaguca caacaacagu uaguaacaug gcagagguac gaucuuauug uuacgaagca      780

aguauaagug auauggcauc ggauaguaga uguccuacac aaggagaggc uuauuuagac      840

aaacaaagug auacacaaua uguuuguaaa agaacacuug uugauagagg uugggguaac      900

gguugcggau uauuugguaa aggcucacug guuacgugug caaaauucgc auguagcaag      960

aaaaugacug gaaaaaguau acaaccagaa aaucuagaau auagaauaau guuaagugua     1020

cacggaagcc aacauucagg aaugauagua aaugacacag gacacgaaac agacgaaaau     1080

agagcaaaag uugaaaucac uccuaauuca ccaagagcag aagcaacacu aggagguuuu     1140

ggaucucuag gccucgacug ugaaccacgc acaggauuag acuuuucaga cuuauacuau     1200

cuuacaauga auaacaaaca uuggcuagua cauaaagaau gguuccacga cauuccacua     1260

ccauggcacg caggagcaga cacaggaaca ccacauugga acaacaaaga agcacuugua     1320

gaauuuaaag augcccaugc caaaagacaa acaguaguag uauuaggauc acaagaaggc     1380

gcuguacaua cugcccuagc cggcgcacua gaagccgaaa uggauggcgc aaaaggaaga     1440

cuaucaagug ggcauuuaaa auguagacuu aaaauggaua aacugcgacu aaaaggagua     1500

ucguacucau uauguacggc agcuuuuaca uuuaccaaaa uaccagcaga aacacuacac     1560

ggaacuguaa cgguggaagu ucaauacgcu ggcacagaug gaccuuguaa aguaccagca     1620

caaauggcag uagacaugca aacuuuaaca ccaguaggaa ggcuaauaac agccaacccg     1680

guaauaacug aaaguacaga aaauucaaaa augaugcuag aauuagaucc uccguucgga     1740

gauagcuaca uaguaauugg uguaggagaa aagaaaauaa cccaucauug gcaccgaagu     1800

ggaucuacaa ucggaaaagc auucgaagca acaguaagag gagccaaaag aauggcugua     1860

cuuggggaua cagcauggga uuuuggauca guagggggag cgcucaauag uuuaggaaaa     1920

ggaauacauc aaauauuugg agcagcuuuu aaaaguuuau uuggaggaau gaguugguuc     1980

ucacaaauau uaauagguac ccuacuaaug ugguuaggac uuaauacuaa aaauggaagu     2040

auaucauuga uguguuuagc auuaggugga guucuaauau uuuuaucaac agcaguaagu     2100

gcagauguag guugcucagu agacuucagc aaaugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  4704
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4704
gggagaaagc uuaccaugaa aaaaaggaga ggagcggaua caucaguagg aauuguagga       60

uuacuguuaa cuacugcaau ggcagcagaa guuacaagaa gaggaagugc cuauuauaug      120

uauuuggaca gaaacgacgc aggagaagca auaucauuuc caacaacauu aggaaugaau      180

aaauguuaua uacaaauaau ggauuuagga cauaugugug augccacaau gucuuaugaa      240

uguccuaugu uagacgaagg aguagaaccu gaugacguug auuguuggug caacacaaca      300

aguacuuggg uaguauaugg aacaugccau cacaaaaaag gggaagcaag gagaucaaga      360

agggcaguua cauuaccauc ucacucuacg agaaaauuac agacaagauc acaaacgugg      420

uuagaaagua gagaauauac aaaacacuug auacgagucg aaaauuggau cuuccggaau      480

ccagguuuug cgcuagcagc agcagcaaua gcauggcuau uaggaaguuc aacaagucaa      540

aaaguaauau acuuagugau gauuuuguua auugcaccug cguacaguau aagauguaua      600

ggagugucua auagagacuu uguagaagga augucaggcg gaacaugggu agauauugua      660

cuagaacacg gaggaugcgu gacaguaaug gcacaagaca aacccacagu agauauagaa      720

uuaguaacca caacaguaag caauauggca gaaguaagaa gcuauuguua ugaggcaagu      780

auaucagaua uggcaaguga uucaagaugu ccaacucagg gagaagcuua uuuagauaaa      840

caaagcgaca cacaauaugu auguaaaaga acacuuguag acagaggaug gggaaacggg      900

ugcgguuuau uuggaaaagg uucauuagua accugugcaa aauuugcaug cucuaaaaaa      960

augacuggaa aaaguauaca acccgaaaau uuagaauaua gaauaaugcu aaguguacac     1020

ggaucacaac auucagggau gauaguaaau gauacgggac augaaacaga ugaaaauaga     1080

gcaaaaguag aaauaacucc caacagucca agagcagaag cgacacuagg uggguuuggu     1140

aguuuagggc uggacugcga accaagaacg gguuuagacu uuucagauuu guauuaucua     1200

acuaugaaca auaaacauug guuaguacau aaagaguggu uucacgacau uccgcuucca     1260

uggcaugccg gugcagacac aggaacacca cauuggaaca auaaagaagc auuaguagaa     1320

uuuaaagaug cacaugcaaa aagacagacc guaguagucu uaggaaguca agaaggagcg     1380

guacacacug cacuagcagg agcacuagaa gcagaaaugg acggagcaaa aggaagacug     1440

ucaaguggac accuaaaaug uagguuaaaa auggauaaac uaagauuaaa agguguuuca     1500

uauucacuau guacagcagc auuuacauuu acgaaaauac cagcagaaac auuacacggu     1560

acaguaacag uagaaguaca guaugcagga acagauggac cuugcaaagu accugcacag     1620

auggcuguag auaugcaaac acuuacacca guaggcagac uaauaacagc aaauccagua     1680

auuaccgaaa guaccgaaaa uucaaaaaug auguuagaac uugauccacc uuuuggagac     1740

agcuauauag uaauaggggu gggagaaaaa aaaauaacac accauuggca uagaagugga     1800

aguaccauag ggaaagcauu ugaagcaaca guuaggggag caaaaagaau ggcgguauua     1860

ggggacacag cuugggacuu uggaaguguu gguggagcau uaaauucauu aggaaaagga     1920

auacaucaaa uauuuggagc agcauuuaaa ucauuauuug gagggaugag uugguuuagu     1980

caaauauuaa uaggaacacu auuaaugugg uuaggguuaa auacuaaaaa uggcaguaua     2040

agcuuaaugu guuuagcacu agggggagug cuaauauuuc uaaguacagc cguaagugca     2100

gacgucggau gcaguguuga uuuuuccaaa ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  4705
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4705
gggagaaagc uuaccaugaa aagaagaggu gcagacacga guguuggaau agugggauug       60

uuacuaacaa cagcaauggc ugcagagguu accagaagag gaucugcaua uuauauguau      120

uuagacagaa acgaugccgg ugaagcaaua aguuucccaa cgacucuagg aaugaauaaa      180

ugcuacauac aaauuaugga cuuaggacau augugcgaug caacaaugag cuaugaaugc      240

ccaauguuag augagggagu agaaccagac gauguagauu guugguguaa uacaacauca      300

acuuggguag uuuacgguac uugccaucau aaaaaaggag aagcaagaag aucaagaaga      360

gcaguaacau ugccuaguca uucaacacgc aaacuccaaa caagaucaca aacaugguug      420

gaaucaagag aauacacaaa acauuuaaua agaguagaaa auuggaucuu ucgaaaucca      480

ggauuugcac uagcagcagc agcaauagca ugguuauuag gaaguucuac aucacaaaaa      540

guaauauacc uagucaugau acucuugaua gccccugcau auucuauaag augcauagga      600

guuaguaaua gagauuucgu ugaaggaaug uccggaggaa cuuggguuga uauagucuua      660

gaacacggag gauguguaac uguaauggca caagauaaac caacuguaga uauagaauua      720

guuacaacaa caguaucuaa uauggcagag guacgaucau acuguuauga ggccaguaua      780

agugauaugg cauccgacuc acgaugucca acacaaggag aagcuuaccu ugauaaacaa      840

agugauacgc aauauguaug uaaaagaaca uuaguagauc gaggaugggg caauggcugc      900

ggauuauuug gcaaaggauc cuuaguaaca ugugcaaaau ucgcauguag caaaaaaaug      960

acaggaaaau caauacaacc agaaaauuua gaauauagaa uaaugcuauc aguacaugga     1020

ucacaacaua gcggaaugau aguuaacgau accggacaug aaaccgacga aaauagagca     1080

aaaguagaaa uaacaccaaa cagcccaagg gcagaagcaa cauuaggcgg auuuggaucc     1140

uuagguuuag acugugaacc acgcacuggu uuggauuuua gugauuuaua cuaccuuaca     1200

augaacaaua aacauugguu aguccacaaa gaaugguuuc augauauacc auuaccaugg     1260

caugcuggug cagauacugg gaccccacau uggaauaaua aagaagcauu aguagaguuu     1320

aaagaugcac augcaaaaag acaaacaguc gucguccuag ggagucaaga aggagcagua     1380

cacacagcac uagcaggagc ccuugaagca gaaauggacg gagcaaaagg aagauuaagu     1440

ucaggacauu uaaagugucg ccuuaaaaug gacaaauuaa gacuaaaagg agucaguuau     1500

ucgcuaugua ccgcagcguu cacguuuaca aaaauuccgg cagaaacucu acaugguacu     1560

guuacuguag agguccaaua ugcaggcaca gacggaccau guaaaguacc agcucaaaug     1620

gccgucgaca ugcaaacacu cacuccagua ggaagauuaa uaacagcaaa uccaguaauu     1680

acagaaucaa cagaaaauag uaaaaugaug uuagaauugg auccacccuu uggagacagu     1740

uauauuguaa uaggagucgg agaaaaaaaa auaacacauc auuggcaucg aucaggauca     1800

acgauaggaa aagcauuuga ggcaaccguc agaggagcua aaagaauggc aguucuagga     1860

gacaccgcau gggauuucgg aucagucgga ggggcacuaa auucucucgg uaaagggaua     1920

caucaaauau uuggagccgc auuuaaaucc uuauucggag guaugaguug guuuucacaa     1980

auccuaauag gaacauugcu aauguggcug ggacuaaaua cgaaaaaugg uaguauauca     2040

uugaugugcu uagcucuagg agguguauua aucuuucuua gcacagcagu uucggcagac     2100

gucggaugua guguagauuu caguaaauga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4706
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4706
gggagaaagc uuaccaugag aagaggagcc gacacuucag ugggaauugu aggauuauua       60

uuaacaaccg caauggcagc agaaguuaca agaaggggau cagcauauua uauguaucuu      120

gauagaaaug acgcagguga agccauuagu uuccccacaa ccuuagguau gaauaaaugc      180

uauauacaaa uaauggauuu aggacauaug ugugaugcaa ccaugaguua cgaaugccca      240

auguuagacg aagggguuga acccgaugau guagauugcu gguguaacac uacaaguaca      300

uggguaguau auggcacaug ucaucacaaa aaaggugaag caagaagauc aagacgagca      360

guuaccuuac cuagccauag uacacgcaaa uuacaaacaa gaucacagac uugguuagaa      420

aguagggaau auaccaaaca cuuaauaaga guugaaaauu ggauauuuag aaaucccgga      480

uuugcauuag cagcagcagc aauagcgugg uuacuaggca gcucaacuag ucaaaaagua      540

auauaucuag uaaugaucuu auuaauagcu ccagcauauu caauaagaug uauaggagug      600

agcaauagag auuuuguaga aggaaugagu ggagguacau ggguagacau aguacuagaa      660

cacggaggcu guguaacagu cauggcacaa gauaaaccaa caguugauau cgaauuggua      720

acaacaacag uaagcaacau ggcggaagua agaagcuacu gcuaugaagc uaguauaagc      780

gacauggcaa gugauucaag auguccuaca caaggagaag cguaucuaga uaaacaaagu      840

gauacacaau auguuuguaa aagaacauua guagauagag gauggggaaa uggaugugga      900

uuguuuggua aagggucauu ggucacuugc gcuaaauuug cauguaguaa aaaaaugaca      960

ggaaaaagca uucaaccaga aaaccuagaa uauagaauaa ugcuuagugu ccauggauca     1020

cagcauucag guaugauagu aaaugacaca ggacaugaaa cagacgaaaa uagagcaaaa     1080

guagaaauaa caccgaacag cccaagagca gaagcuacuu uaggaggauu uggaaguuua     1140

ggacuagacu gugaaccaag aacaggcuua gauuuuagug acuuauauua uuugacgaug     1200

aauaauaaac acugguuagu acacaaagaa ugguuccaug auauaccauu gccauggcau     1260

gcaggugcag auacaggaac accacacugg aauaacaaag aggcccuggu agaguucaaa     1320

gaugcacaug caaaaaggca aacaguugua guauuaggau cucaagaagg ugcaguucac     1380

acugcccuag ccggagcauu agaagccgaa auggauggag cuaaaggaag auugaguagc     1440

ggacaucuua aaugcagauu aaaaauggau aaauuaagac ucaaaggugu aagcuauagu     1500

uuauguacag cagccuuuac auuuacuaaa auacccgcag aaacacuaca uggaacaguu     1560

acaguugaag uacaauacgc aggaacggau gguccaugua aaguaccugc ucaaauggca     1620

guagacaugc agacacuaac gccgguaggu agauuaauaa cagcgaaucc cguuauaacu     1680

gaaucaacag aaaauucaaa aaugaugcua gaauuagauc caccuuuugg agacagcuau     1740

auaguaauag gaguaggaga aaaaaaaaua acacaucacu ggcauagauc aggcagcaca     1800

auugguaaag cuuuugaagc uacaguucga ggagcaaaaa gaauggcagu acuaggagau     1860

acagccuggg auuuuggaag cguaggugga gcuuuaaacu cuuugggaaa agguauacau     1920

caaauuuuug gagcugcuuu uaagaguuug uuugguggaa ugucgugguu uagucaaaua     1980

uugauaggca cauuacucau gugguuagga cuaaauacaa aaaacggauc aauaagucua     2040

auguguuuag cacuaggagg aguauuaaua uuuuuaucua cagcaguaag cgcagacguu     2100

ggauguucag uagauuucag uaaaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4707
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4707
gggagaaagc uuaccaugag gggagcagau acaucaguag gaauaguagg auuauuauua       60

acaacagcaa uggcagcaga aguuacaagg aggggaagug ccuacuacau guacuuagau      120

agaaacgaug caggagaagc aauuaguuuu ccuacaacau uaggaaugaa uaaaugcuau      180

auacaaauaa uggacuuagg ucauaugugc gacgcaacua ugucuuacga augcccaaug      240

uuagaugaag gaguagaacc agacgaugua gacuguuggu guaauaccac aaguacgugg      300

guaguauaug gaacuuguca ucacaagaaa ggagaagcaa gaagauccag gagagcagua      360

acuuuaccau cacacucaac gagaaaauua cagacaagaa gccaaacaug guuagaauca      420

agagaauaua caaaacacuu aaucagagua gaaaauugga uauuucgaaa uccaggauuc      480

gcacuugcgg cagcugcuau agcauggcuc uuaggaaguu cuaccagcca aaaaguaauu      540

uauuuaguaa ugauacuuuu aauagcaccc gcauauucaa uaagguguau cggaguuucu      600

aauagagauu uuguggaagg aaugucaggc ggaacuuggg uggacaucgu uuuagaacac      660

ggaggaugug uuacaguaau ggcucaagau aaaccaacug uagauauaga auuaguaacu      720

acgacagugu caaauauggc agaaguaaga ucuuauuguu augaggcuuc aaucagugac      780

auggcaucag auaguagaug uccaacacaa ggagaagcau auuuagacaa acaaagugac      840

acacaauaug ucuguaaaag gacauuagua gacagagggu ggggaaaugg auguggacuc      900

uuuggaaaag gaucucuagu aacuugcgcu aaauuugcau guucaaagaa aaugacagga      960

aaaucaauac aaccagaaaa ucucgaauau agaauuaugu uaucaguaca uggcucacaa     1020

cauaguggaa ugauaguuaa ugauacagga caugaaacag augaaaacag agcaaaagua     1080

gaaauuacac cuaacucacc uagagccgaa gccacacuag gugguuuugg aaguuuagga     1140

cuagauugug aaccaaggac aggucuagau uuuagcgacu uauauuauuu aacaaugaau     1200

aauaaacauu gguuggucca uaaagaaugg uuccacgaua uuccauuacc uuggcaugca     1260

ggagcagaua caggaacacc acauuggaau aacaaagaag cauuaguuga auuuaaagau     1320

gcacaugcua aaagacaaac uguaguugua cuuggauccc aagaaggagc aguucauaca     1380

gcauuagcgg gugcacuuga agcagaaaug gauggagcaa aagguagacu aucaagcgga     1440

caucugaaau gucgacuaaa aauggauaaa cugagauuga aaggaguuag uuauaguuua     1500

ugcacagcag cauucacauu uacaaaaauc ccagcagaaa cauuacaugg aacaguaacu     1560

guagaaguac aauaugccgg aacugaugga cccuguaaag uucccgcaca gauggcggua     1620

gacaugcaga cauuaacucc aguagguaga uuaauaacgg caaauccugu cauaacggaa     1680

ucuacagaaa auucaaaaau gauguuggaa uuagacccac cauuuggaga uuccuacaua     1740

guuauaggag ucggugaaaa gaaaauaaca caucauuggc acaggagcgg aaguaccauu     1800

gguaaggcuu uugaagcaac aguaagagga gcaaaaagaa uggcaguuuu gggagauaca     1860

gcaugggauu ucgguagugu uggaggagcu cuuaauucuu uaggcaaagg aauacaucaa     1920

auauucggag cagcuuuuaa aagucuauuc ggagggauga guugguuuag ccaaauacua     1980

auuggaacgc uacugaugug guuaggacuc aacacuaaaa acgggagcau uaguuuaaug     2040

ugccuagcau uagguggagu acuaauauuu cuaucaacag caguuucagc cgaugucggc     2100

uguucaguag auuuuuccaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4708
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4708
gggagaaagc uuaccauggg ugcagauaca ucaguuggaa uaguaggacu gcuauuaaca       60

acagcaaugg cggcagaagu aacaagacgg ggaucagcuu auuacaugua ucuagauaga      120

aaugaugcag gagaagcaau aucauuccca acgacacuag gaaugaacaa auguuacauc      180

caaauaaugg accuaggaca uaugugcgac gccacuauga gcuaugaaug cccaaugcua      240

gaugaaggag uagaaccgga ugauguugac uguuggugua auacgacauc aacuugggua      300

guauacggaa cuugucauca uaagaaagga gaagcaagac gaaguagacg agcaguaaca      360

cuaccaucac acaguacaag aaaauuacaa acaagaaguc agacauggcu ugaaagcaga      420

gaauauacaa aacauuuaau aagaguagaa aauuggauuu uuagaaaucc agguuucgcc      480

uuagcugcag cugcgauagc auggcucuug ggaucuagca cuucacaaaa aguaauauac      540

uuaguaauga uuuuacuaau ugcaccagca uauucaauca gauguauugg agucagcaau      600

agggacuuug uagaaggcau gucaggugga acguggguag auaucguacu agaacacgga      660

gggugcguaa caguaauggc acaggauaaa ccaacgguag acauagaauu aguaacgaca      720

acgguaucaa auauggcaga aguaagaagu uacuguuacg aggcaucuau uagugacaug      780

gcuucagacu cacgaugucc aacacaagga gaagcauauu uagauaaaca aagugauacg      840

caauacguau gcaaaagaac auuaguagac agaggguggg guaauggaug cgguuuauuu      900

ggaaaaggau cauuaguaac augugcaaaa uuugcauguu cuaagaaaau gacaggaaaa      960

aguauccaac cagagaauuu agaauacagg auuauguuaa guguacacgg aucacaacau     1020

ucagggauga uaguaaauga cacaggacau gaaacugaug aaaaucgagc aaaaguagaa     1080

auaacuccca auucaccaag agcugaagcu acauuagggg gauuuggauc acuaggacua     1140

gauugugaac cgagaacagg auuagauuuu agugauuuau auuauuuaac aaugaacaau     1200

aaacacuggu uaguacauaa agaaugguuu caugacauac cauuaccgug gcacgcaggg     1260

gcagauacug gaacaccaca uuggaauaau aaagaggcac ugguugaguu caaagacgcu     1320

cacgcaaaaa gacaaacagu agucguguua ggcucucagg aaggagcagu acauaccgcg     1380

uuagcaggcg cacuagaagc agaaauggau ggcgcuaaag gcagacuuag cagcggacac     1440

uugaaaugca gacuaaaaau ggacaaauua cgguuaaaag gcgugaguua uucauuaugu     1500

acagccgcau ucacauucac aaaaauaccu gcugaaacuu uacaugggac agugacggua     1560

gaaguacagu augcggggac agacggaccu uguaaaguac cugcacaaau ggcaguagau     1620

augcaaacuu uaacaccagu aggaagauua auaacagcaa auccaguaau uacagaaagu     1680

acagaaaaua guaaaaugau gcuagaauua gacccaccuu uuggcgacuc auauauaguc     1740

auaggaguag gugaaaagaa aauaacacac cauuggcaca ggaguggauc uacaauagga     1800

aaagcauuug aagccacagu aagaggagcc aaaagaaugg caguacuagg agacacagcu     1860

ugggauuuug gaagcgucgg aggagcacua aauaguuuag gaaaaggaau acaucaaaua     1920

uucggagcag ccuucaaauc auuauuuggu gguauguccu gguuuaguca aauauuaaua     1980

ggaacauuau uaaugugguu aggacuaaau acuaaaaacg guaguaucuc acuuaugugu     2040

uuagcccugg guggaguauu aauauuuuua ucaaccgcug uaucagcuga uguuggaugu     2100

ucaguagauu ucucaaaaug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4709
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4709
gggagaaagc uuaccauggc agauacaucc guaggaauag uaggacuuuu auuaacaaca       60

gcaauggcag cggaaguaac aagaagaggu ucagccuauu acauguauuu ggauaggaau      120

gacgcaggag aagcaauuag cuucccuaca acacuuggga ugaacaaaug uuauauacaa      180

aucauggacc uaggacauau gugugaugca acuaugaguu acgaaugucc aaugcuugau      240

gagggaguag aaccagacga cgucgauugu uggugcaaua cuacaaguac uuggguagua      300

uaugguacau gucaucauaa aaaaggagaa gcaagacgcu caaggagagc aguaacauua      360

ccuagucacu caacaagaaa acugcaaaca cgaagucaaa cauggcucga gagccgagaa      420

uauacaaaac aucuaauuag aguagagaac uggauauuca gaaauccagg auucgccuua      480

gcggcagcag caauagcaug guuacuagga aguucaacau cacaaaaagu aauauaucua      540

gucaugauuu uauuaauagc uccugcauau ucuauacgau guauaggagu caguaauagg      600

gauuuuguag aaggaaugag uggagguacc uggguagaua uuguauuaga acacggaggc      660

uguguaacug uuauggcaca agauaaaccu acaguagaua uagaacuagu aacgacaaca      720

gugagcaaca uggcugaagu gagaucauau uguuaugaag cuagcauaag cgauauggcc      780

agugauucua gauguccaac ccaaggagaa gcauacuuag auaagcaauc ugauacccaa      840

uauguaugca aaagaacacu aguagaccga ggauggggca auggaugcgg ucuauucgga      900

aaaggaagcu uagugacaug ugcaaaauuu gcauguucca aaaaaaugac aggcaaaagc      960

auacaaccag aaaauuuaga auacaggaua augcuaucag uacauggauc ucaacauagc     1020

ggaaugauag uaaaugauac aggacacgaa acugacgaaa auagagccaa aguagagauc     1080

acacccaaca guccaagagc agaagcaacg cuaggaggau uuggaagccu cggauuagau     1140

ugugaaccaa gaacaggacu agauuucagc gaucuuuacu auuuaaccau gaacaacaaa     1200

cauugguuag uacauaaaga gugguuucau gauauaccgu uaccuuggca ugcaggagca     1260

gauacaggaa caccacacug gaauaauaaa gaagcccuug uagaauuuaa agacgcacau     1320

gcaaaaagac aaacgguagu uguauuaggu agucaagaag gagcaguaca uacagcacua     1380

gcuggugcau uagaagcaga aauggacgga gcaaaaggaa gauuaagcag cggacaucua     1440

aagugcagac uaaaaaugga uaaacuucgc cuaaaaggag uaucauacuc auuauguacu     1500

gccgcuuuua cauucaccaa aauaccugcu gaaacuuuac acggaacagu cacuguagaa     1560

guacaauaug cagguacaga ugguccaugu aaagugcccg cucaaauggc aguagauaug     1620

caaacacuaa caccuguagg aagauuaaua acagcaaacc cuguaauaac agaaucaaca     1680

gaaaacucga aaaugaugcu cgaacuagac ccaccguuug gagauaguua cauuguaauu     1740

gguguaggag aaaaaaaaau uacacaucau uggcaccguu cagggaguac uauaggaaaa     1800

gcauuugaag caacuguaag aggagcaaaa agaauggcag uauuagguga uacagccugg     1860

gacuucggua guguaggagg agcauugaac ucccuaggaa aaggcauuca ucaaauauuu     1920

ggagcagcau uuaaaaguuu auuuggagga augucauggu uuagucaaau auuaauaggc     1980

acgcuacuua ugugguuagg acuaaauaca aaaaacggaa guauuagccu aaugugcuua     2040

gcuuuagggg gcguguuaau uuuuuugagc acagcaguua gcgcagacgu aggauguagu     2100

guugauuuuu caaaaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4710
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4710
gggagaaagc uuaccaugga cacuagugua ggaauaguag gucuacuacu aacaacagca       60

auggcagcag aaguuacucg aagagguagc gcauauuaua uguauuuaga uagaaaugac      120

gcaggagaag cuauaaguuu cccaacaaca cuaggaauga acaaauguua cauucaaaua      180

auggaccugg gacauaugug cgaugcaacc augucuuacg aauguccuau gcuugacgaa      240

ggaguagaac cugaugaugu ugacuguugg uguaauacaa ccagcacuug gguaguauau      300

ggaacauguc aucauaaaaa aggggaagca agacgaucaa gaagggcagu gacauugcca      360

agucacucaa cuagaaaauu acaaacuaga ucacaaacau gguuggaauc aagagaauac      420

acaaaacauu uaauuagagu ggaaaauugg auauuccgaa acccaggauu cgcauuagcg      480

gcagcagcaa uagcuugguu auugggaagc ucaacgaguc aaaagguuau auaccuagug      540

augauacuau uaauagcucc agcauauucc auaagaugca uaggaguaag uaaccgagac      600

uuuguagaag gaaugagugg aggaacaugg guagauauag uauuggaaca uggaggaugu      660

guaacaguaa uggcacaaga uaaaccaaca guagauauag aacuaguuac aacaacaguu      720

ucaaauaugg ccgaaguaag aucguacugu uaugaggcua gcauaucaga cauggcaucu      780

gauucaagau guccaacuca aggcgaagca uacuuagaua aacagaguga cacgcaauau      840

guaugcaaaa gaacacucgu ggauagagga ugggguaacg gguguggccu guucggcaaa      900

gguagucuag uaacaugugc uaaauuugcu uguucaaaaa aaaugacagg aaaaucaaua      960

caaccggaaa accuagaaua cagaauaaug uuaagugucc acgggaguca acauucagga     1020

augauuguua augauacagg ucaugagacu gaugaaaaua gagcaaaagu agagaucaca     1080

ccaaauucac cacgagcaga agcaacacua ggugguuuug gaaguuuagg auuagauugu     1140

gaaccaagaa caggauuaga uuuuucagac uuguauuacu uaacaaugaa uaauaaacac     1200

ugguuagucc acaaagaaug guuucaugau auuccacugc cauggcaugc aggagcagau     1260

acaggcacac cacacuggaa uaacaaagaa gcauuaguug aauuuaaaga ugcacacgcu     1320

aaaagacaaa caguagucgu auuaggaagu caagaaggag ccguucacac agcacuagca     1380

ggugcauuag aagcugaaau ggacggagcu aaagguagau uaucaagugg acaccuaaaa     1440

ugcagacuga aaauggacaa auuaagacua aaaggaguaa guuauagcuu auguacagcg     1500

gcauuuacau uuacaaaaau accggcagaa acauuacaug guacaguaac uguagaagua     1560

caauacgccg gaacagaugg accaugcaaa guaccugcuc aaauggccgu cgauaugcaa     1620

acccuaacac cgguuggaag acucauaaca gcaaauccag uaauaacaga gucaacagaa     1680

aauaguaaaa ugauguuaga auuagaccca ccauuuggug acucguacau agugauagga     1740

guuggugaaa aaaaaauaac acaccauugg cacagaagug gaagcacuau uggaaaagca     1800

uuugaagcaa cuguuagagg cgcaaaaaga auggcaguuc uuggagauac agcaugggau     1860

uuuggaagug uuggaggagc auuaaacagu uuaggaaaag gcauacauca aauuuuugga     1920

gcagcuuuua aaucacuauu uggaggaaug ucaugguuuu cacaaauauu aauugguacu     1980

uuacuaaugu gguuaggacu aaauacuaaa aaugguagua uaaguuuaau gugucuagca     2040

uuaggcggag ucuuaaucuu cuuaucaaca gcaguaagcg cagacguagg guguagugua     2100

gacuucucaa aaugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4711
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4711
gggagaaagc uuaccaugac cagcguugga auaguagguu uacuauuaac aaccgcaaug       60

gcagcagaag ugacaagaag aggaagugca uacuauaugu acuuagaucg uaacgaugca      120

ggugaagcua uaucuuuucc aacaacguua ggaaugaaua aauguuacau acaaauaaug      180

gacuuaggac auauguguga cgcaacuaug ucauaugaau guccaauguu agaugaagga      240

guugaaccag acgacguaga uuguuggugu aauacuacca guacaugggu aguauaugga      300

acaugccauc auaaaaaagg agaggcuaga agaaguagga gagcaguaac auuaccaucu      360

cauucaacaa gaaaacuuca aacacgauca caaaccuggc uagaaagccg agaauacaca      420

aaacaucuaa uacgagugga aaauuggaua uuuagaaauc caggauucgc acuagcugca      480

gccgcuaucg cauggcuacu agguaguuca acaucacaaa aaguaauuua cuuaguaaug      540

auccuacuca uugccccggc auauucaaua agaugcaucg ggguuagcaa cagagacuuu      600

guugaaggca ugucgggagg cacaugggua gauauuguac uugagcacgg agggugcgua      660

acuguuaugg cucaagauaa accaacaguu gacauagaac uaguaacaac aacaguauca      720

aacauggcgg aaguaagaag uuauugcuau gaagcaagca uaucugacau ggcaucagau      780

ucuagauguc caacacaagg agaagcuuau uuagauaaac aaucagacac acaauacgua      840

uguaaaagaa cauuaguaga cagaggcugg ggaaauggau guggauuguu uggaaaagga      900

ucauugguaa caugugccaa auucgcaugu aguaaaaaaa ugacuggaaa aaguauccaa      960

ccagaaaauu uagaauaucg aauuauguua agcguccaug guucccaaca uucagguaug     1020

auaguaaacg auacaggaca cgagacugau gaaaauagag caaaagugga aauaacucca     1080

aauaguccaa gagcagaagc aacuuuaggu gguuuuggau cccucggauu ggauugugaa     1140

ccaagaaccg gauuagacuu cagugaucua uacuacuuaa caaugaauaa uaaacacugg     1200

cuuguccaca aagaaugguu ccacgacauc ccauuaccau ggcacgcagg cgcagauaca     1260

ggcaccccac auuggaauaa uaaagaagca cuaguagaau ucaaagacgc acaugcaaaa     1320

agacaaacgg uaguaguauu aggaagucaa gaaggagcug uacauacagc ccuagcugga     1380

gcacuagaag cagaaaugga cggagcaaaa gguagauuaa guaguggaca ccugaaaugc     1440

agauuaaaaa uggauaaacu aagguuaaaa ggaguuagcu auucuuuaug uacugcagca     1500

uuuacauuca cuaaaauacc agcagaaacc uuacauggaa cagugacagu agaaguacaa     1560

uaugcaggaa cugauggacc auguaaagua ccagcacaaa uggcaguuga uaugcaaaca     1620

uuaacuccag uagggagacu uauaacagca aauccaguaa uaaccgaauc aacagaaaau     1680

agcaaaauga uguuagaauu agacccaccu uucggcgaca gcuacauagu cauaggaguu     1740

ggugaaaaaa aaauaaccca ucauuggcau agaaguggaa gcacaauagg caaagcauuu     1800

gaagcaacug ucagaggggc caaaagaaug gcaguucuag gagauaccgc augggacuuc     1860

ggaaguguag gaggagcacu aaacaguuua ggaaaaggaa uacaucagau auuuggggca     1920

gcauucaaaa gucuauuugg gggcaugagc ugguuuucac aaauuuuaau uggaacauug     1980

uuaauguggc uaggauuaaa uacaaaaaau gguagcauaa gcuuaaugug ucuagcucua     2040

ggaggaguac ugauuuuuuu aagcacagcu guuagugcug auguagggug uucuguagau     2100

uuuucaaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4712
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4712
gggagaaagc uuaccaugac aaguguuggu auaguggggu uauuauuaac gacugcaaug       60

gcagcagcag uaacaagaag aggaucagcu uauuauaugu auuuagacag aaaugaugca      120

ggggaagcaa ucucauuucc caccacucua gguaugaaua aauguuauau ucaaauaaug      180

gaucucgguc auauguguga ugcuacuaug aguuaugaau guccaauguu agaugaagga      240

guugaaccag augaugucga cuguuggugc aauacaacaa guacuugggu aguauaugga      300

accugucauc auaagaaagg agaagcuaga cguaguagga gagcaguaac acuuccaagu      360

cauucaacaa gaaaacuaca aacuagaagc caaacauggu uagaaaguag agaauauaca      420

aaacaccuaa uaagaguaga aaacuggauu uuuagaaacc caggauuugc ccuggcagcu      480

gcugcuauag ccugguuacu gggauccagu acaucacaaa aaguaauaua cuuaguaaug      540

auauuauuga uagcucccgc uuauagcaua agauguauug gcgucucuaa uagagauuuc      600

guagaaggga ugaguggugg gacaugggua gauauaguau uagaacacgg agguugcgua      660

acaguaaugg cacaagacaa accaacagua gauauagaau uagugacaac aacagugagc      720

aauauggcug aaguacgauc auauugcuau gaagcaucaa ucucagauau ggcuucagac      780

ucaagauguc caacgcaagg agaagcauau cuagacaaac aaagugauac acaauacgua      840

uguaaaagaa cauuaguaga uagagguugg gguaaugguu guggacuauu uggaaaaggc      900

uccuuaguua caugugcuaa auucgcuugc uccaagaaaa ugacaggaaa aucaauacaa      960

ccugaaaauc uugaauacag aauaaugcua aguguacaug gaagccaaca uagugggaug     1020

auaguaaaug auacgggaca ugaaacugau gaaaauagag caaaaguaga aauaacucca     1080

aauagcccaa gagcagaagc aacuuuaggc ggauucggca gccuuggauu agauugugaa     1140

ccaagaacag gcuuagauuu uagcgacuua uauuacuuaa caaugaauaa caaacauugg     1200

uuaguccaua aagaaugguu ucacgauaua ccauuaccau ggcaugcggg ugcugauacc     1260

gguacaccac auuggaauaa caaagaagca uugguagagu ucaaagacgc acacgcaaaa     1320

aggcaaacag ucguagucuu gggaagccaa gaaggagccg uacauaccgc auuagcagga     1380

gcacuagaag cagaaaugga cggagcaaaa ggaagauuaa gcaguggaca uuuaaaaugu     1440

cgauuaaaaa uggauaaacu uagauuaaaa ggagucucau auucacuuug uacagcagca     1500

uuuacuuuua caaaaauacc ugcagaaaca uuacauggga cagugacagu ggaaguacaa     1560

uaugcaggaa cagauggacc auguaaagua ccagcucaaa uggcuguuga uaugcaaaca     1620

cuaacaccag uaggacgacu aauaacugcu aauccaguaa uaacagaauc aacugaaaau     1680

agcaaaauga ugcuagaacu ugacccgccu uuuggugaca guuauauagu uauagguguc     1740

ggugaaaaga aaaucacaca ucacuggcau aggagugggu caacaauagg gaaagcguuu     1800

gaagcaacag ucaggggugc aaaaagaaug gcuguacuag gagauacagc augggauuuu     1860

ggaaguguag gaggggcauu gaauagucua ggaaaaggua uacaucaaau auuuggggca     1920

gcauuuaaaa gucuauuugg cggaauguca ugguuuucac aaauucuaau aggaacuuua     1980

cuuauguggc ucggauugaa uacgaaaaau ggaucaauca gcuuaaugug uuuagcuuua     2040

ggaggugugc uaauauuuuu aaguacagcc guaucagccg auguaggaug uucuguagau     2100

uuuaguaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4713
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4713
gggagaaagc uuaccaugag aggagcagau acaaguguug ggauaguagg acugcuucua       60

acuacugcaa uggccgcugc gguaacgaga cgaggaagug cauauuacau guauuuagac      120

agaaacgaug caggagaagc uauaaguuuu ccaacaacgc uagguaugaa caaaugcuac      180

auacagauaa uggaucuagg acacaugugu gacgcaacua ugucauacga augcccaaug      240

uuagaugaag guguagaacc agaugauguc gacuguuggu gcaacacaac uucgacaugg      300

guaguauaug gaacauguca ucauaaaaaa ggagaagcac gccgaucaag aagggcaguc      360

acauuaccau cacacaguac aaggaaauua caaacaagaa gucaaacaug gcuugaauca      420

agagaauaua caaaacacuu aauuagggua gaaaauugga uuuucagaaa uccaggauuu      480

gcauuagcag ccgcagcaau agcuugguua uuaggaagua guacaagcca aaaaguaauu      540

uauuuaguua ugauacuuuu aauagcucca gcauauucaa uacgauguau agguguauca      600

aacagagauu uuguugaagg aaugagugga ggaacauggg uagauauagu ucuagaacau      660

ggagguugug uaacaguaau ggcacaggac aaaccaacag uagacauaga auuaguaaca      720

acaacaguuu caaauauggc agaaguaaga ucuuauuguu acgaagcaag uauaagcgau      780

auggcuagcg auucacgaug ucccacacaa ggagaagcuu aucuagauaa acaaucagau      840

acacaauacg uauguaaacg aacauuggua gacagaggau ggggaaaugg auguggauua      900

uuuggaaaag guagccuagu aacaugcgca aaauucgccu guaguaaaaa aaugacagga      960

aaaucaauuc aaccagaaaa cuuagaauau agaauaaugc uauccguuca cggcucgcaa     1020

cauucaggga ugauuguaaa ugauacagga caugaaacag augaaaacag ggcuaaggua     1080

gaaauaacuc ccaauucacc aagagcugaa gcaacauuag gcggguuugg aucacucggu     1140

cuagacugug aaccacgaac uggauuagau uuuucagauc ucuacuaucu gacuaugaau     1200

aauaaacauu gguuagucca uaaagaaugg uuucacgaua uaccacuacc cuggcaugca     1260

ggugcagaua cuggaacacc ucacuggaau aauaaagaag cucuuguaga guuuaaagau     1320

gcacaugcaa aaagacaaac aguaguagua uuaggaaguc aagaaggagc aguacacaca     1380

gcauuagccg gugcucuaga agcagaaaug gacggagcaa aaggcagacu cucaagugga     1440

cauuuaaaau gucgauuaaa aauggacaaa uuaagguuaa aagguguaag uuacagucua     1500

uguacagcug cauuuacauu uacaaaaaua ccugcugaaa cgcuccaugg aacagucaca     1560

guugaagucc aauaugccgg aaccgauggc ccauguaaag ucccugcaca aauggcggua     1620

gacaugcaaa cauuaacacc aguugguaga uuaauaaccg caaauccggu uauaacagaa     1680

aguacagaaa auuccaaaau gauguuagaa uuagauccac cauuuggaga cuccuauaua     1740

guaauuggag uaggagaaaa aaaaauaaca caucauuggc acagaucagg uucaacaaua     1800

ggaaaagcgu ucgaagcgac agugagagga gcaaaaagaa uggcuguauu aggagauaca     1860

gcuugggacu uuggguccgu aggcggugca cuuaauucac uuggaaaagg cauacaucaa     1920

auuuucgggg cggcuuuuaa aucacuuuuc ggaggaaugu caugguuuuc acagauauua     1980

auaggaacau uauuaaugug guuaggacuu aauacaaaaa auggaagcau uaguuuaaug     2040

ugccuagcgc ugggaggugu acuuauauuu cuuucaaccg caguaagugc ugauguaggg     2100

uguucuguag acuuuuccaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4714
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4714
gggagaaagc uuaccaugag aggacuguca ucgagaaaaa gaagaagcca ugauguccua       60

acaguucaau uucuaauauu agggauguua cuaaugacgg gaggaguaac uagacgugga      120

ucagcauauu auauguaccu agauagaaac gaugcuggug aagcaauauc auuuccaaca      180

accuuaggaa ugaauaaaug uuacauucaa auuauggauu ugggucauau gugcgaugca      240

accauguccu augaaugucc aauguuagac gaaggagugg aaccagauga uguagauugu      300

uggugcaaua caacgucaac uuggguagua uauggaacuu gucaucauaa gaaaggagaa      360

gcaagaagau cuagacgagc aguaacauua ccaucacauu caacuagaaa acuacaaaca      420

agaagccaaa caugguuaga gaguagagaa uacaccaaac aucuaauaag gguugaaaau      480

uggauauuuc guaauccugg uuucgcauua gcagcagcag cuauagcaug guuauuagga      540

agcaguacau cacaaaaagu aauauacuua gucaugauau uacuaaucgc accagccuau      600

ucaauaagau guauaggagu uaguaauaga gauuuugugg aagguaugag ugguggaaca      660

uggguugaca ucguacuaga acauggaggg uguguuacug uaauggcaca agauaaaccg      720

acaguugaua ucgaauuagu gacaacaaca guaucuaaua uggcagaagu aagaagcuau      780

uguuaugaag cuagcauauc cgauauggca ucagacagca gaugucccac acagggagaa      840

gcauaucuug auaaacaguc agacacacag uauguaugca aaagaacauu aguagacaga      900

ggguggggaa acggaugugg uuuauuugga aaaggaucau uaguaaccug ugcuaaauuu      960

gcaugcagca agaaaaugac uggcaaaagu auacaaccgg aaaaucuaga auaccgaaua     1020

augcucagug uucacggauc acaacauuca gguaugauug uaaacgauac uggacaugaa     1080

acagaugaaa acagagcaaa agucgaaaua acuccaaaca guccacgagc agaagcuacu     1140

uuaggcgguu uuggaucccu uggauuagau ugugagccac gaacuggauu agauuucagu     1200

gauuuauauu auuuaacaau gaauaauaaa cacugguuag uacacaaaga augguuccau     1260

gauauaccau uaccuuggca ugcaggagcu gauacaggga caccacacug gaauaacaaa     1320

gaagcccuag uagaauuuaa agaugcacau gcgaaacgac aaacaguugu ugucuuagga     1380

ucucaagaag gugcaguaca uacagcauua gcaggagccc uagaagcaga aauggacggu     1440

gcuaaagggc gauuaucuag cggacauuua aaauguagau uaaaaaugga uaaauuaaga     1500

cuaaaaggug uuagcuauag uuuauguaca gccgcauuca cauucacuaa aauaccugca     1560

gagacucuac auggaacagu aacuguagaa guacaauaug cagguacuga cggaccuugc     1620

aaaguuccag cacaaauggc aguugacaug caaacacuaa cuccaguagg aagauuaaua     1680

acggcgaacc caguuauaac agaaucaacu gaaaauagca aaaugaugcu ugaacuagau     1740

ccaccauuug gagacucaua uauaguaaua ggaguaggcg aaaagaaaau aacacaucac     1800

uggcauagaa gugguagcac uauugguaaa gcauuugaag caacuguaag aggugcaaaa     1860

agaauggcag uauugggaga uacugcaugg gauuuuggau caguaggcgg ggcacuaaau     1920

ucacuuggga aaggaauaca ucaaauauuu ggagcagcau uuaaaucuuu auuuggaggc     1980

augucauggu ucucacaaau auugauugga acccuacuua uguggcuagg uuuaaacacu     2040

aaaaauggau caauuucauu gaugugcuua gcauugggag gaguauuaau auucuuaagc     2100

acagcaguaa gugcagacgu aggaugcagu guugacuuua guaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4715
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4715
gggagaaagc uuaccaugag aggauuaagu agcagaaaaa gaagaagcca ugacguguua       60

acaguacaau uucugauacu gggaaugcua cuaaugacug gagcaguaac uagaagagga      120

agcgcauauu auauguacuu agauagaaau gaugcuggug aagcuauauc cuuuccaacc      180

acauugggaa ugaauaaaug uuauauacaa auuauggacu uaggacauau gugugaugcu      240

acaaugucau augagugucc uaugcuagau gaaggaguag aaccagauga cguagauugu      300

ugguguaaua caacgucaac auggguagua uauggaacuu gucaucauaa gaaaggagaa      360

gcaagaagau caaggagagc ugugacacua ccuucacaua gcacaagaaa acuucaaacc      420

agaagccaaa cauggcucga aucaagagaa uauacuaaac aucuaauacg uguugaaaau      480

uggauauuua gaaacccagg auucgcacuu gccgcagcag caauagcaug guuauugggu      540

ucgaguacau cucagaaagu aauauacuua guaaugauuc uauuaaucgc accagcauau      600

ucaauaagau gcauaggagu gagcaaccga gauuuuguug aaggcaugag uggaggaacg      660

uggguugaua uaguucugga acacggagga uguguuacag uaauggcaca agacaaacca      720

acaguagaca uagaauuggu caccacaaca guaaguaaua uggcagaagu aagaagcuac      780

uguuacgagg ccaguauuag ugauauggca ucagauucca gauguccuac acaaggagaa      840

gcauauuuag acaaacaauc agauacacaa uauguaugua aaaggacauu aguugacaga      900

ggauggggaa auggaugcgg auuauuuggu aaaggaucau uagugacaug ugcuaaauuu      960

gcaugcagua agaaaaugac aggaaaaagc auacaaccgg aaaaucuuga guauaggaua     1020

auguuaagug uacacggauc acaacauucu ggaaugauug uaaaugauac uggacaugaa     1080

acagaugaaa aucgagcaaa aguagaaaua acucccaauu caccaagagc ugaggcaaca     1140

cuaggaggau uugggagcuu aggauuagau ugugaaccua ggacuggacu ugauuuuagu     1200

gaucuauauu auuuaacgau gaauaauaaa cauuggcuag uacauaaaga augguuucau     1260

gacaucccau uaccauggca ugccggggca gauacaggaa caccacacug gaauaacaaa     1320

gaagcguugg uagaauuuaa agaugcacau gcaaaaagac aaacaguugu aguauuagga     1380

agccaagagg gagcaguaca uacugcacuu gcaggagcau uagaagcaga aauggauggu     1440

gcaaaaggaa gacuaucauc agggcauuua aaauguagac uuaaaaugga uaaauuaaga     1500

cuaaaaggcg uaagcuauuc auuauguaca gcagcauuua cuuucacaaa aauaccagca     1560

gaaacauuac auggaacagu aacaguggaa guacaauaug cgggaacaga uggaccaugu     1620

aaaguuccag cacaaauggc aguugauaug caaacauuaa caccaguugg aagacuaaua     1680

acagcaaauc caguaauaac ugaauccaca gaaaacucaa aaaugaugcu ugaacuagau     1740

ccaccauuug gagauaguua uauagugauu ggcguugggg agaagaaaau aacacaucau     1800

uggcauagau caggaucaac aauaggaaaa gcauuugaag caacuguaag aggagcaaaa     1860

agaauggcag uucuaggaga uacagcaugg gauuuuggaa gcguuggagg agcgcuaaau     1920

agcuugggga aagggauuca ccaaauauuu ggagcugcau uuaaaucauu auuuggagga     1980

augaguuggu uuagccaaau acuaauagga accuuauuga ugugguuagg auuaaauacu     2040

aaaaacggaa guauaaguuu aaugugucua gcacuagggg gaguuuuaau auuuuuaagu     2100

acagcaguau cagccgaugu aggcuguagu guagauuuuu caaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4716
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4716
gggagaaagc uuaccaugag aauuauaaau gcaaggaaag agaaaaaaag aagaggugca       60

gauacaagcg uugguauagu aggacuacua uuaacaacug caauggcggc agaaguaaca      120

agacgugguu cagcuuauua cauguauuua gauagaaacg augcuggaga ggcaauauca      180

uuuccuacaa cuuuaggaau gaauaaaugc uauauacaaa uaauggauuu aggacauaug      240

ugugacgcaa caaugaguua ugaaugcccu augcuagaug aaggaguuga acccgacgau      300

guagauuguu ggugcaauac uacaaguaca uggguuguau auggaacaug ucaucacaaa      360

aaaggagagg cuagaagauc aagaagagca guaacauuac cuucacacag cacacgaaaa      420

cuacaaacaa gaucacaaac augguuagaa ucaagagaau acacaaaaca ucuaauuagg      480

guagaaaauu ggaucuuuag aaauccuggc uuugcacuug cagcagcagc aauagcuugg      540

uuauuaggua gcaguacauc ucaaaaagua auauauuuag uaaugauacu acuaaucgca      600

ccagcauaca gcauaaggug cauaggagua ucuaauaggg auuuuguaga aggaauguca      660

ggaggaacau ggguugacau uguacuagaa cauggugguu guguaacagu aauggcgcaa      720

gauaaaccaa cagucgauau agaacuagua acaacaaccg uaucaaauau ggcugaaguu      780

agaucauauu guuaugaagc aucaaucagc gauauggcau cagacagcag augcccaacu      840

caaggagaag cuuauuuaga uaagcaauca gauacucaau acguauguaa aagaacacua      900

gucgauagag gaugggguaa cggauguggu uuauucggca aaggaucauu aguuaccugu      960

gccaaauuug cauguucaaa aaaaaugacu ggaaaaagca uacaacccga gaauuuagaa     1020

uauaggauaa uguugucugu ccauggaucu caacacucag guaugauagu uaaugauaca     1080

ggacacgaaa cagaugaaaa uagggcuaaa guagaaauaa cacccaauag uccucgagcu     1140

gaagcaacac ucggaggauu uggaagucua ggacuagacu gugaaccaag aacaggacua     1200

gacuuuagug aucuauauua uuuaacaaug aacaacaaac auuggcuagu acauaaagaa     1260

ugguuucacg acauaccucu accuuggcac gcaggcgcug auacaggaac accacacugg     1320

aacaauaaag aagcacuagu ugaauuuaaa gaugcucacg caaaaagaca aacagucguc     1380

guacuaggaa gucaagaagg agcuguacau accgcgcuag ccggcgcuuu agaagccgag     1440

auggacggag caaaaggaag auuaucaucg ggacacuuaa aauguagauu aaaaauggac     1500

aagcuaagau uaaaaggagu cucauauuca cuaugcacag cagcauuuac auucacaaaa     1560

auuccugcgg aaacacuaca uggaacagua acuguugaag uacaauaugc aggaacagau     1620

ggacccugca agguuccugc acaaauggcu guagauaugc aaacacuaac accaguagga     1680

agacuaauaa cagcaaaccc aguaauaaca gaaucuacag aaaauucaaa aaugauguug     1740

gaauuagauc caccauuugg ggauaguuau auaguaauag guguaggaga aaaaaaaaua     1800

acacaucacu ggcauagaag uggcuccacg auaggaaaag cauucgaagc aacaguacga     1860

ggagcaaaaa gaauggcagu acuaggagac acagcauggg auuucggauc cguuggagga     1920

gcauugaauu cauuagguaa agguauccac caaauauuug gagcggcauu uaaaucguua     1980

uucggaggaa ugaguugguu uucucaaauc cuaauuggua cacuacucau gugguuaggu     2040

uuaaacacaa aaaaugguuc aaucucauua augugcuuag cgcuuggagg aguacuaaua     2100

uuucucagua cagcugugag ugcugacgua gguuguugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4717
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4717
gggagaaagc uuaccaugau uauuaaugca agaaaagaaa agaaaagaag aggugccgau       60

acaagcguag gaauaguagg acuacuacuu acaacagcaa uggcagccga aguaacaaga      120

cgcgguucug cuuauuauau guaucuagac agaaaugaug cuggagaagc cauuaguuuc      180

ccaacaacau uaggaaugaa uaaauguuac auacaaauaa uggaccuagg acacaugugu      240

gacgcaacaa ugucauauga augcccaaug uuagacgaag guguugaacc agacgaugua      300

gauuguuggu guaauacaac aucaacaugg guuguuuaug gaacaugcca ucauaagaaa      360

ggcgaggcua gaagaucaag acgagcaguc acauuaccua gucauaguac aagaaaauua      420

caaacaagau cucagacaug gcuugaauca agagaauaua caaaacacuu aauuagaguu      480

gaaaauugga ucuuuagaaa cccugguuuu gcacuagccg cagcugcaau agcuuggcua      540

uuaggaucau cuacaucaca aaaaguaaua uauuuaguca ugauucuacu aauagcacca      600

gcauauucaa ucagaugcau aggagugagc aauagagauu uuguggaagg caugucugga      660

gggacauggg uagauauugu auuagaacau ggagggugcg uaacuguuau ggcacaggau      720

aaaccaacag uagacauaga auuaguuaca acaacaguuu caaauauggc ggaaguuaga      780

aguuacuguu augaagcauc aauuucagau auggcaucag auaguagaug uccaacacaa      840

ggggaggcuu auuuagauaa acaaagugac acucaauaug uauguaaacg aacauuagua      900

gauagaggau ggggcaacgg uugugguuua uuuggcaaag gaaguuuagu aacaugugca      960

aaauuugcgu guaguaagaa aaugacaggc aaaucaauac aaccagaaaa cuuagaauau     1020

cgaaucaugc uuaguguaca cggcucacaa cauucaggaa ugauuguaaa ugacacugga     1080

caugagacag augaaaacag agcaaaagug gaaauuacac cuaacucacc aagagcagaa     1140

gcuacguuag gagguuucgg aucucuugga uuagacugug aaccaagaac aggcuuagac     1200

uucucugauc uauauuaucu uacaaugaau aacaaacauu gguuaguaca uaaagagugg     1260

uuucaugaua uaccauuacc auggcacgca ggagcagaua cagggacgcc acauuggaac     1320

aauaaagaag cucuaguaga auuuaaagau gcacacgcaa aaagacaaac aguaguuguu     1380

uuagguucuc aagaaggagc cguacacaca gcacuagcug gagccuuaga agcagaaaug     1440

gacggggcua aagguagauu aucaucugga cacuuaaaau guagauuaaa aauggauaaa     1500

uuaagauuaa aaggaguaag uuauagccug uguacagccg cauuuacuuu uaccaaaaua     1560

ccagcagaaa cauuacaugg uacaguaacc guagaagugc aguaugcagg aacagauggg     1620

ccuuguaaag ucccagcuca aauggcagua gauaugcaaa cacuuacccc aguaggcaga     1680

cuuauaacug caaauccagu gauaacagaa ucaaccgaga acuccaaaau gaugcuagaa     1740

cuagauccac cauucggaga uaguuauaua guuaucggag ucggagaaaa gaaaauaacu     1800

caucauuggc auagaucagg uagcacgaua ggaaaagcau uugaagcaac agucagaggu     1860

gccaaaagaa uggcaguauu gggagauaca gcaugggacu uuggaagugu cggaggagca     1920

cuaaauagcu uaggaaaagg aauacaucaa auuuucggag cagcauuuaa aucuuuauuu     1980

ggaggaauga gcugguucuc acaaauacuu auaggaacau ugcugaugug guuaggacuc     2040

aauaccaaaa acggaagcau aucauuaaug ugucuagcac ugggaggugu cuuaauauuc     2100

cuaucgacag caguaucagc agauguggga uguugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  4718
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4718
gggagaaagc uuaccaugau caacgcaaga aaagaaaaaa aaagacgagg agcagacaca       60

aguguaggaa uaguaggauu acuuuugaca acugcaaugg cagcggaagu aacaagaaga      120

ggaagugcau auuauaugua uuuagaccgc aaugaugccg gugaagcgau aucauuccca      180

acuacuuuag gaaugaauaa auguuauaua caaauaaugg auuuaggaca uaugugugau      240

gcaacgauga gcuaugaaug ccccauguua gaugaaggag uagaaccaga cgauguagac      300

uguuggugua auacaacaag uacaugggua guauauggaa cgugucauca caaaaaagga      360

gaagcaagga gaaguaggag agcaguaacc uuaccauccc auagcacucg aaaauuacaa      420

acaagaagcc aaacaugguu agaaagucga gaauauacaa aacauuuaau aagagucgaa      480

aauuggauau uuagaaaucc aggauucgca uuagcagcag cugcaauagc auggcuauua      540

gguucaucaa caagucaaaa aguaauauau uuaguaauga uuuuacuaau agcaccagca      600

uauucaauaa gauguauugg aguaagcaac cgcgauuuug uagaaggaau gucaggagga      660

acauggguag acauaguacu agaacaugga ggguguguca caguaauggc acaagauaaa      720

ccaacgguag auauagaauu aguaacgacc accguuucua acauggcuga aguacgauca      780

uauugcuacg aagcaucaau aagcgacaug gcaucagauu cuagaugucc aacucaagga      840

gaagcguauu uggauaaaca auccgauaca caauacguau gcaagaggac ucuaguagau      900

agggguuggg gaaacggaug uggguuauuu ggaaaaggaa guuuaguuac augugcuaag      960

uuugcgugua guaaaaaaau gacaggaaag ucaauucaac cagaaaauuu ggaauacaga     1020

auaauguuaa gcguacaugg cucacaacac agcggaauga uagugaauga cacaggacau     1080

gaaacagaug aaaauagagc aaaaguagaa auaacaccaa auaguccaag agcagaagcg     1140

acacuaggag guuuugguuc auuaggucua gauugcgaac cucgaacagg uuuagacuuu     1200

ucagaucugu auuauuuaac aaugaacaau aaacauuggu uaguccauaa agaaugguuu     1260

cacgauauac cuuuaccgug gcacgccgga gcagacacag gaacacccca uuggaauaau     1320

aaagaagcac uaguagaguu uaaagacgcu caugcaaaaa gacaaacugu aguaguuuug     1380

gguagccaag aaggagccgu acauacagca uuagcaggag cgcuagaagc agagauggac     1440

ggcgcuaaag gaagauuauc cuccggacau uuaaaauguc gauuaaaaau ggauaaauua     1500

agacuaaaag gaguaucaua uaguuuaugc accgcagcuu uuacauuuac aaaaauaccu     1560

gcagaaacuc uacauggaac cguaacagua gaaguucaau augcaggcac agauggacca     1620

uguaaaguuc cugcacaaau ggcuguagau augcaaacac ucacacccgu uggaagauua     1680

auaacagcga acccaguaau aacugaaagc acagaaaaua guaaaaugau guuagaauua     1740

gacccaccau ucggagauag uuauauagua auagguguag gagaaaaaaa aauaacucac     1800

cauuggcaca gaucaggguc aacuauugga aaagcauuug aggcaacagu aagaggagcg     1860

aaacguaugg caguauuagg agacaccgcg ugggacuuug guagcguagg aggagcauua     1920

aacucguuag gaaaaggaau ccaucagaua uucggagcag ccuuuaaaag ucucuucgga     1980

ggaaugagcu gguucucaca aauuuuaaua ggaacauuac uaauguggcu aggauuaaau     2040

acaaaaaaug gcaguauauc auuaaugugc uuagcucuag gaggaguacu aauauucuua     2100

aguacugcag ucucagcuga ugugggaugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  4719
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4719
gggagaaagc uuaccaugaa cgcaagaaaa gaaaaaaaac gucguggugc cgauacaagu       60

guaggaauag uaggauuauu guuaacaaca gcaauggcag cagaaguaac acgaagagga      120

ucagcauauu acauguauuu ggauagaaau gaugcgggag aagcaauauc auuuccaacu      180

acacuuggaa ugaauaaaug uuauauucaa auuauggauu uaggacauau gugcgacgcu      240

acaaugucau acgaaugccc gauguuagac gaaggggugg aaccagauga cgucgauugu      300

ugguguaaua caacgucaac guggguagua uauggaacau gccaucacaa aaaaggcgaa      360

gcuagaagaa gucguagagc aguaacauua ccaagccaua guacaagaaa auuacaaaca      420

cgaucccaaa cuugguuaga aucaagggaa uacacuaaac auuugauaag aguagaaaau      480

uggauauuua gaaauccggg uuucgcccuu gcggcagcag caauagcaug guuacuagga      540

ucuucaacaa gucaaaaagu aauauauuua guaaugauac uuuuaauagc accagcuuau      600

agcauaagau gcauuggugu aaguaauagg gacuuuguag aagguauguc agguggaaca      660

uggguagaua uaguacuaga acauggaggu ugcguaacag uuauggcaca agauaaacca      720

acggucgaca uagaacuagu uacaacaaca guuagcaaua uggcagaagu aagaucauau      780

uguuacgaag caucuauaag cgacauggca agugauucua gauguccaac acaaggagag      840

gcauaccuug auaaacaaag ugauacucaa uauguaugca aaagaacauu aguagaucga      900

ggauggggaa acgggugugg ucuauuuggu aaaggaucau uaguaacgug ugcaaaauuu      960

gcauguucga aaaaaaugac aggaaaauca auacaaccag aaaacuuaga auauagaaua     1020

augcucagcg uacaugguuc acaacauuca ggaaugauug uaaaugauac aggucaugaa     1080

acagaugaaa accgagcaaa aguagaaauu acuccaaaua guccaagagc ugaagcaaca     1140

cuuggagguu uuggaagucu aggacuagac ugcgaaccaa gaacuggauu agauuuuucu     1200

gaucuauacu aucuaacaau gaacaauaag cauugguuag uacauaaaga gugguuucau     1260

gauauuccac uaccuuggca ugcaggugcu gauacaggaa ccccacauug gaauaauaaa     1320

gaagcuuugg uagaauuuaa agaugcacau gcaaaaagac aaacuguagu aguauuagga     1380

ucacaagaag gagcaguaca caccgcacua gcaggagcuu uagaagcuga aauggaugga     1440

gccaaaggaa gauuauccag uggacauuua aaaugcagau uaaagaugga caaacuacga     1500

cuaaaaggag uaagcuauuc auuauguaca gcagcauuua cauuuaccaa aauaccugcu     1560

gaaacucuac acggaacagu aacaguagaa guacaauaug cggguacaga uggaccaugu     1620

aaaguaccag cucaaauggc uguagauaug cagacuuuaa caccgguagg aagauugaua     1680

acugcaaauc cuguaauaac ugaaucaaca gaaaauagua aaaugauguu agaauuagau     1740

ccaccauucg gagauucaua cauaguaauu ggugucgggg aaaaaaaaau aacccaucau     1800

uggcauagau caggaaguac aauaggaaaa gcuuuugaag caacaguaag aggagcaaaa     1860

agaauggcgg ugcucggaga uacagcuugg gauuuuggca guguaggugg ugcacuaaau     1920

ucauuaggua aaggaauaca ucaaauuuuu ggagcugcgu uuaaaucacu auucggagga     1980

augucuuggu uuucucaaau auuaauuggg acuuuguuaa ugugguuagg ucuaaauaca     2040

aaaaaugggu caauaucacu aauguguuua gcacugggag gaguauuaau uuuuuuaagu     2100

acagcaguaa gcgcagacgu aggauguuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4720
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4720
gggagaaagc uuaccauggc cagaaaagaa aaaaaaagaa gaggagcuga uacaucagua       60

ggaauaguag gacuauuguu gacaacagca auggcagcag agguaacaag acgagguagc      120

gcauauuaca uguauuuaga uagaaacgau gcaggagaag cuauaucauu cccaacuaca      180

uuaggaauga acaaaugcua uauacaaauu auggauuuag gacauaugug ugaugcaaca      240

augaguuaug aaugcccuau guuagaugaa ggaguagaac ccgacgaugu ugacugcugg      300

ugcaauacga caaguacaug gguuguauau gguacauguc aucauaaaaa aggagaagca      360

agacgaagua gacgcgcagu cacauuaccg agccauagca cgcguaaacu acaaacacga      420

ucacaaacuu ggcuagagag uagagaauau acuaaacauu uaaucagagu cgaaaacugg      480

auauucagga aucccggauu ugcauuagca gcagcagcaa ucgcauggcu auuaggcuca      540

aguacuaguc aaaaaguaau auauuuagua augauauuac uaauugcccc agcauacagu      600

auaagaugua uagguguaag caaccgagac uucguagaag gaaugucugg aggaacaugg      660

guugacauug uacuagaaca cggagguugu gucacaguga uggcgcaaga uaaaccuaca      720

guugauauug aacuaguuac aacaacagua aguaacaugg cugaaguaag aucauacugu      780

uaugaagcau cuauaaguga uauggcuagu gacagucgau guccaacaca aggcgaagca      840

uacuuagaua aacaaucaga uacucaauau guauguaaaa gaacacuagu cgacagaggg      900

uggggaaacg gauguggacu auuuggaaaa ggaucgcuag ugacaugugc aaaauuugca      960

ugcucaaaaa aaaugacagg caaaucaaua caaccugaaa auuuagaaua ccggaucaug     1020

uuaucagucc augguucaca acauagcgga augauaguca augauacagg acaugaaacg     1080

gaugaaaaca gggcaaaagu agaaauaacu ccaaacaguc caagagcaga agccacauug     1140

ggaggcuuug gaagcuuagg auuagacugc gaaccgcgua caggcuuaga uuucuccgau     1200

cuuuauuauc uaacuaugaa uaauaaacau ugguugguac auaaagaaug guuucacgac     1260

aucccauuac cuuggcaugc aggagcagau acaggaacac cacauuggaa caauaaggaa     1320

gcauuaguag aauucaaaga cgcacaugca aaaagacaaa caguaguagu acuaggauca     1380

caagaaggag caguccauac ugcucucgca ggagcguuag aagcagaaau ggacggagca     1440

aaagggcguu ugagcucagg acauuuaaaa ugcagauuaa aaauggauaa auuacgauua     1500

aaaggaguau cuuacucgcu auguaccgca gcuuucacau ucacaaaaau accagcagaa     1560

accuuacaug ggacaguaac gguugaagug caauaugcag ggacagaugg cccauguaaa     1620

guacccgcuc aaauggcagu ugacaugcaa acucuaacac caguaggaag auuaauuaca     1680

gcaaacccag uaauuacaga aaguacugaa aacaguaaaa ugauguugga acuagaucca     1740

ccauuuggag acagcuauau aguuaucggc guaggugaaa aaaaaauaac acaucauugg     1800

cauagaagug guaguacgau aggaaaagcu uucgaagcua cgguaagagg agcaaaaaga     1860

auggcagucu ugggagauac cgcaugggau uuugguagug uugguggugc auuaaauuca     1920

uuagguaaag gaauacacca aauauuugga gcagcauuca aaucuuuauu cggagguaug     1980

aguugguuuu cacaaauauu gauaggaaca uuacuaaugu ggcuaggcuu aaauacaaaa     2040

aaugguucca uaaguuuaau guguuuagca uuagggggug uguuaauauu uuuaucuacu     2100

gcaguuagug cugauguagg cuguugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4721
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4721
gggagaaagc uuaccaugag aaaagaaaag aaaagaagag gugcagacac uucaguagga       60

auugugggau ugcuacuuac aacugcaaug gcagcagaag uaacacgacg uggaucugca      120

uacuauaugu acuuagacag gaaugacgcu ggugaagcaa uaaguuuucc aacaacauua      180

ggcaugaaca aaugcuacau acaaauaaug gaccuaggac auauguguga cgcaaccaug      240

aguuacgaau guccaauguu agaugaagga guagagcccg augauguaga uugcuggugu      300

aauacaacaa gcacaugggu aguguaugga acaugucacc auaagaaagg ugaagcaaga      360

agaucaagga gagcaguaac ucuuccauca cauucaacgc gaaaauuaca aacaagguca      420

caaacauggu uagaaaguag agaauauacg aaacaccuca uaagaguaga aaauuggaua      480

uucagaaauc ccggcuuugc acuagcagca gcagcaauag caugguuacu gggaaguagu      540

acaucacaga aaguaauaua uuugguaaug auauuacuua uagccccagc auauucaauu      600

agauguauag gaguuucgaa uagagauuuc guggaaggca ugaguggagg aacgugggua      660

gacauaguuu uagaacaugg aggauguguc acagugaugg cacaagauaa accgacagua      720

gauauagaau uagugacaac uacaguaagu aauauggcag aaguuagaag uuauuguuau      780

gaagcaucca uaagcgauau ggcuucagau ucaagauguc cgacucaagg agaagcauau      840

uuagauaaac aaagugacac acaauacgua uguaaaagga cucuaguaga uagaggaugg      900

gguaauggau guggguuauu ugguaaagga ucuuuaguca caugugcaaa auucgcaugc      960

aguaagaaaa ugacaggcaa aaguauccag ccagaaaauu uggaauauag gauaaugcua     1020

ucaguacacg guucucaaca uuccggaaug auugucaaug auacagguca ugaaacagau     1080

gaaaacaggg cgaaagugga gauuacacca aacucaccaa gagcggaagc aacacuaggu     1140

ggauuugguu cauugggauu agauugcgaa ccaagaacag gauuagauuu uucagaucug     1200

uacuauuuaa cuaugaacaa caaacauugg uuaguacaua aagaaugguu ucaugauaua     1260

ccauuaccau ggcaugcggg agcagauaca ggaacgccac acuggaacaa caaagaagca     1320

cucguagaau uuaaagacgc acaugcgaaa cgacaaacag uaguaguauu aggaagccag     1380

gaaggagcag uacauacagc acuagcaggc gcauuagaag cagaaaugga uggagcaaaa     1440

gguagauuaa guaguggaca uuuaaaaugu agguuaaaaa uggauaaacu aagauuaaaa     1500

ggaguaaguu auaguuuaug cacugccgca uucacauuua ccaaaauacc ugcagagaca     1560

uuacauggca caguaacagu ugaaguacaa uacgcaggca cagaugggcc augcaaagug     1620

cccgcucaaa uggcaguaga uaugcaaacc uuaacgccag uuggcagauu aauaacugca     1680

aacccaguua uaacugaauc gacagaaaac agcaaaauga uguuagaacu agauccgccu     1740

uuuggagauu cauauauagu aauuggagua ggagaaaaga aaauaacaca ucacuggcau     1800

agaaguggaa gcacaauagg aaaagcauuu gaagcgacag uaaggggagc gaaaagaaug     1860

gccguauuag gagauacagc augggacuuc gguaguguag gaggagcuuu aaauagucua     1920

ggaaaaggaa uacaucaaau auuuggagca gcuuuuaaau cacuauuugg ugguauguca     1980

ugguuuucac aaauauuaau aggaacauua cuaauguggc uaggccuaaa uaccaaaaau     2040

ggaaguauaa guuuaaugug uuuagcauua ggugguguau uaauauuucu aaguaccgca     2100

guaucagcag auguuggaug uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4722
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4722
gggagaaagc uuaccaugaa agaaaagaaa agaagaggug cagauacauc cguaggcaua       60

guagguuugu uauuaacaac agcaauggcu gccgaaguga cuagacgugg aagugcauac      120

uacauguauu uagauagaaa ugaugcugga gaagcuauuu cauuuccuac cacauuagga      180

augaauaaau guuacauaca aauaauggau cuaggucaca ugugcgaugc cacaaugagc      240

uacgaauguc caauguuaga ugagggagua gaaccagacg auguugauug uugguguaau      300

acaacaucaa cguggguagu uuaugguaca ugccaucaua agaaaggaga agcuagaaga      360

ucaagacgag caguaacauu accaagucac ucaacuagaa aacuacaaac aagaagccaa      420

acauggcuag aaagcagaga auauacuaaa caccuuauua gaguagaaaa uuggauauuu      480

aggaacccag gauuugcuuu agcggcugcu gcaauugcau ggcugcuugg aucaaguaca      540

ucucagaaag ucauauaucu gguaaugauc uuauuaauag ccccugcaua caguaucagg      600

ugcauaggag uaucgaaucg ugauuucgua gaaggaauga gcgggggaac auggguugac      660

auaguauuag agcauggagg auguguuaca guaauggcac aagauaaacc aacugucgau      720

auagaauuag uaaccaccac aguaagcaac auggcagaag uaagaaguua uuguuaugaa      780

gcaucaauaa gcgauauggc aucugacuca cgauguccaa cacaagggga agcauaucuc      840

gauaaacagu cagauacuca auauguuugu aaaagaacuc uaguagaucg aggcugggga      900

aacggaugcg gucucuucgg aaaaggauca uuaguaacau gugcaaaguu cgcauguagu      960

aagaaaauga caggaaaauc cauacaacca gaaaauuuag aauauagaau uauguuaagc     1020

guacaugguu cucaacacuc aggaaugaua guaaaugaua caggccauga aacagacgaa     1080

aacagagcca aaguagagau aacaccaaac aguccacgag cugaagcuac auuaggaggg     1140

uucggaucau uaggacugga uugugaaccc agaacugguc uagacuuuuc agaccuuuac     1200

uauuuaacaa ugaauaauaa acauuggcua guacacaaag aaugguuuca cgacauuccg     1260

uuaccauggc augcgggugc agauacaggc acaccacauu ggaauaauaa agaagcucua     1320

guagaguuua aagaugcaca ugcaaaacga caaacagucg uaguacuagg uagucaagaa     1380

ggagcaguac auacugcauu agcuggagca uuagaagcag aaauggaugg agcaaaagga     1440

aggcuaucau ccggucaucu aaaauguaga cuaaaaaugg auaaacugag auuaaaggga     1500

gucagcuauu cauuauguac agcagcauuc acauucacca aaaucccagc agagacuuua     1560

cauggaacag uaacagucga aguacaauau gcagguacag auggaccaug uaaaguucca     1620

gcacaaaugg cuguagauau gcaaacguua acacccguug guagacuaau aacagcuaac     1680

ccaguuauua cagaaagcac agaaaauuca aaaaugaugu uagagcuaga cccaccauuc     1740

ggugauagcu auauugucau aggagucgga gaaaagaaaa uaacacacca uuggcacaga     1800

ucaggaucaa caauaggaaa agccuuugaa gcuacaguaa gaggagcaaa acgaauggcc     1860

guauuaggug auacagcaug ggauuuugga ucaguuggag gagcacuuaa cucucuaggu     1920

aaaggaauac aucaaauauu uggagcagcu uucaaaucuc uauuuggugg uaugaguugg     1980

uuuagucaaa uauuaauugg uacauuacua augugguuag gucuaaauac uaaaaauggu     2040

ucaauaaguu uaauguguuu agcauuagga ggcguuuuaa uauuuuuaag uacagcagua     2100

agcgcugacg uuggcuguug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4723
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4723
gggagaaagc uuaccaugga aaagaaaaga agaggagcag auacaagugu aggaauuguu       60

ggacuauuau uaacuacagc cauggcagca gaaguaacga gaagggguag ugcuuauuac      120

auguaucuag acaggaauga ugcaggggaa gcaauaucuu ucccaacuac auuaggcaug      180

aauaaauguu auauucaaau aauggaucua ggacauaugu gugaugcaac aaugucauau      240

gaaugcccaa uguuagauga ggguguggaa cccgaugaug uagauugcug guguaauaca      300

acaagcacau ggguuguaua uggaaccugc caucauaaga aaggagaagc aagaagaucu      360

aggagagcag ugacguuacc aagucauucc acaagaaaau uacaaacaag aucgcagaca      420

ugguuagaaa gcagagaaua cacaaaacac uuaauaagag uagaaaauug gauuuucaga      480

aauccagguu uugcauuagc agccgcagca auagcauggc uccuaggauc aaguacaucu      540

caaaaaguua uauauuuagu caugauacua cuaauugcuc cugcauauag uauaagaugc      600

auagguguua guaauagaga uuuuguagaa gggaugucag gagguacaug gguagauaua      660

guauuagaac auggaggaug uguaacugua auggcacaag acaaaccuac aguagauaua      720

gaacucguaa cuacaaccgu aaguaauaug gcagagguaa gaucauauug uuacgaagca      780

ucaauaucag auauggcauc agauaguaga ugcccuacgc aaggagaagc auaucuagau      840

aaacaaagcg auacucaaua uguauguaaa cgaacuuuag uagauagagg guggggaaau      900

ggauguggac uauuugguaa aggaucuuua guaacuugug caaaauuugc uuguucaaag      960

aaaaugacug gaaaaucaau ucaaccagaa aauuuagaau aucgaauaau guuaucagua     1020

cauggaucgc aacacagcgg aaugauagua aacgauaccg gacacgaaac ugacgaaaac     1080

agagcaaaag uagagauaac acccaauagu ccaagagcag aagcuacacu aggaggauuu     1140

ggcagccuug gacucgacug ugagccaaga acgggauuag acuuuagcga ucuauauuac     1200

uuaacaauga auaacaaaca uugguuaguc cauaaagaau gguuucauga cauuccacua     1260

ccauggcacg cuggugcaga uacuggaaca ccgcacugga auaauaaaga agcccuaguu     1320

gaauuuaaag augcacaugc aaaaagacaa acagucguag uauuagguuc ucaggaagga     1380

gcaguucaua cagcauuagc cggugcauua gaagcagaaa uggauggagc aaaaggaaga     1440

uuaaguucug gacaucugaa augucgacua aaaauggaca aacuaagauu aaaaggagua     1500

ucauacucau uauguacagc agcauuuaca uucacuaaaa uaccagcaga aacauugcac     1560

gguacaguaa caguagaagu gcaauaugcu ggaacugaug gcccguguaa aguaccggca     1620

caaauggcug uagauaugca gacauuaaca ccaguaggaa gauuaauaac agcaaauccu     1680

guaauaacag aaagcacaga aaacaguaag augauguuag aauuagaccc acccuucggg     1740

gacagcuaua uaguuauagg aguuggagaa aagaaaauaa cacaucacug gcauagguca     1800

ggaucaacaa uagggaaagc auucgaagcg acuguuagag gagcaaaaag aauggcggua     1860

uugggagaua cugcauggga uuucggauca guaggaggag cauugaauag ucuaggcaaa     1920

gguauacacc aaauauucgg ggcagcauuu aaauccuuau uugguggaau gaguugguuu     1980

agucaaauuu uaauaggaac uuuacugaug ugguuaggac uaaauacuaa aaauggaucc     2040

aucucauuaa ugugucucgc gcuaggagga guacuaauuu ucuuaucuac agcaguauca     2100

gcggacguag gaugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4724
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4724
gggagaaagc uuaccaugaa aaaaagaagg ggagcugaua caagcgucgg aauaguagga       60

cuauuauuaa caacagcaau ggcagcagaa guaacacgaa gaggcagcgc uuauuauaug      120

uauuuagaua gaaaugaugc aggugaagcc auaucauuuc caacaacucu aggaaugaau      180

aaauguuaca uacaaauaau ggauuuagga cauaugugug augcaacaau gaguuaugaa      240

ugcccaaugc uggaugaagg uguugaaccu gaugauguag auuguuggug uaauacaaca      300

ucaacauggg uaguauacgg aacaugccau cacaagaaag gagaagcaag aagaucaaga      360

agagcaguaa cacuaccaag ucacucuaca agaaaauuac aaacaagaag ucaaacgugg      420

uuagaaucaa gagaauauac aaaacacuug auacgaguag aaaacuggau cuuuagaaau      480

ccaggauucg cccuugcagc cgcugcaauu gcaugguuac uagguaguag uacuagucaa      540

aaaguuauau accuugucau gauauuauua auagcuccag cauacucaau aagaugcaua      600

ggcguaagua auagagauuu ugucgaagga auguccggag gaacaugggu ugacauagua      660

uuagaacacg gaggaugugu aacaguaaug gcacaagaua aaccaacugu ugauauugag      720

uuaguuacaa cuacaguauc aaauauggca gagguacgaa guuauuguua cgaggcaagu      780

auaucugaua uggcaucuga uucuagaugu ccaacucaag gagaagcaua ucuugacaaa      840

caaucugaua cacaauaugu cuguaaaaga acauuaguag aucguggaug ggggaacgga      900

uguggauuau ucggaaaagg aucacuagua accugugcua aauuugcaug uuccaaaaaa      960

augacuggua aaucaaucca accagaaaau uuagaauaca ggauaauguu aucaguccac     1020

gguagucaac auagcgguau gauaguuaau gauacaggac augaaacaga ugaaaaucga     1080

gcaaaaguug aaauaacccc aaauucacca agagcagaag ccacacuagg gggcuuugga     1140

ucauugggau uagacugcga accucgaaca ggauuggacu ucucugauuu auauuaucua     1200

acgaugaaca auaaacacug guuaguacac aaagaauggu uucacgauau accucuacca     1260

uggcaugcag gugcagacac aggaacacca cauuggaaca auaaagaggc acuaguagaa     1320

uucaaagaug cgcacgccaa acgacaaaca gucguaguuu uagguucaca agaaggagcu     1380

guacauacag cuuuagcagg ggcguuagaa gcagaaaugg auggugcgaa aggacguuug     1440

ucaucugguc auuuaaaaug ucgauugaaa auggauaaac uacgacuaaa aggaguaagc     1500

uacucacuuu guacugcggc uuuuacauuu acaaaaauac cagcagaaac ccugcaugga     1560

acuguuacug uagaaguaca auaugcuggc acagacggac cuuguaaagu uccagcccaa     1620

auggcaguag auaugcaaac acuaacucca guaggaagac uuauaacagc aaauccagug     1680

auuacagaau caacagaaaa uaguaaaaug auguuagaac ucgauccacc auuuggagau     1740

agcuacauag ucauaggagu aggcgaaaaa aaaauaacac accauuggca uagaagcgga     1800

aguaccauag gaaaggccuu ugaagcgaca guaagaggag cuaaacgaau ggcaguacua     1860

ggggauacgg caugggacuu ugguagcgua ggaggagcau uaaacucucu cggaaaagga     1920

auacaccaaa ucuuuggagc ugcauuuaaa ucauuauuug guggcaugag uugguuuagc     1980

caaauccuaa uaggaacauu auuaaugugg uuagguuuaa acacaaaaaa uggauccauu     2040

ucauugaugu gucuggcacu uggaggagua uuaaucuuuc uuuccacugc aguaagcgca     2100

gauguaggau guugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4725
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4725
gggagaaagc uuaccaugaa gcgaagagga gcagauacuu caguaggaau aguaggacua       60

uuauuaacaa cagcaauggc cgcagaaguc acaagaagag gaucagcaua cuauauguac      120

cuagauagaa acgaugcugg agaagcaaua ucguucccaa caacacuagg aaugaauaaa      180

uguuauauuc agauaaugga ucucggacau augugcgaug caacaauguc auaugaaugu      240

ccaauguuag augaaggagu cgaaccugau gauguagauu guugguguaa uacaacauca      300

acauggguag uauauggaac augucaucac aaaaagggcg aggcuagaag aucccguaga      360

gccgucacau uaccaucgca uaguacaaga aagcuacaaa cacguucuca gacauggcua      420

gaaaguagag aauauacaaa acauuuaauc cgagucgaaa acuggauauu ccgcaaucca      480

ggguuugcac uagcugccgc agcuauugca uggcugcuag guucaucaac gucacaaaaa      540

guuauauacc uagugaugau auuauuaauc gcaccagcau auaguauuag auguauaggu      600

guaaguaaua gagacuuugu agaagguaug agcggaggaa caugggucga uauaguauua      660

gaacauggcg gaugugugac cguaauggca caagauaaac ccacgguaga uauagaauug      720

gucacaacua cuguaucaaa uauggcagaa guaagaucau acugcuacga agcaucaauc      780

ucagauaugg ccucagauuc cagauguccg acccaaggag aagcauaucu agacaaacaa      840

ucggauacac aauauguaug uaaacgaacg cuaguugaua gaggaugggg aaauggaugu      900

ggauuauuug gaaaaggaag uuuagucaca ugugcaaaau ucgcaugcag caagaaaaug      960

accggaaaau ccauacaacc agaaaaucua gaauauagaa uaaugcuauc aguacacgga     1020

agccagcaua guggaaugau uguaaaugau accggacaug aaacugacga aaauagagca     1080

aaaguagaaa uaacaccaaa uaguccaaga gcagaagcaa cauuaggggg auucggauca     1140

cuagguuuag acugcgaacc acgaaccgga uuagauuuca gugacuuaua cuauuuaaca     1200

augaauaaua aacauugguu aguacacaaa gaaugguuuc augacauacc auuaccaugg     1260

caugcaggag cagacacagg aacaccacac uggaacaaua aagaagcauu aguagaauuu     1320

aaagaugcac augcaaaacg gcaaacagua guaguauuag gaagucaaga aggcgcugua     1380

cacacagcac uagcuggagc auuggaagca gaaauggacg gagcaaaagg aagguuauca     1440

aguggacacu uaaaauguag auuaaagaug gauaaacuaa gacuaaaagg aguaaguuau     1500

ucauuaugca cagcugcuuu uacauuuacu aaaaucccag cagaaacacu gcacgggaca     1560

guaacaguug aaguacaaua ugcagguacu gauggaccau guaaaguacc ugcucaaaug     1620

gcaguugaua ugcaaacauu aacaccagua gggagauuaa uuacugcaaa cccaguaaua     1680

acagaaucua cugaaaauuc aaaaaugaug uuagaauuag auccaccguu uggagauagu     1740

uauauaguaa uaggaguugg agagaaaaag auaacacauc auuggcacag aagcggauca     1800

acuauaggaa aagcauuuga agccacagua agaggagcaa aaagaauggc aguguuagga     1860

gauacagcau gggauuuugg aucaguggga ggugcacuua auucauuagg aaaagguauu     1920

caccaaauau ucggagcagc cuuuaaaagu cuuuucgggg guaugaguug guucagccaa     1980

auacuaauag gaacacuacu uaugugguua ggauuaaaua cuaaaaaugg uaguauaucu     2040

uuaauguguu uagcauuagg cggaguacug auauuuuuau caacagcggu uagcgcugac     2100

guggguugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4726
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4726
gggagaaagc uuaccaugag gcguggcgca gauacaucag uagggauugu aggauuauua       60

uuaacaacag caauggcagc agaaguuaca agaagaggaa gugcauacua cauguauuua      120

gauagaaacg augccggaga agcaauaucc uuuccaacaa cauuaggaau gaauaaaugu      180

uauauacaaa uaauggacuu aggacacaug ugugacgcaa cuaugucaua ugaaugccca      240

augcuagaug aaggagucga accugaugac guagauugcu gguguaauac aacaaguaca      300

uggguaguau auggaacaug ucaucauaag aaaggcgaag caagacgauc aaggagagca      360

guuacacugc ccucacacuc aacaagaaaa cuacaaacaa gaucacaaac uugguuagaa      420

ucuagagaau auacaaaaca ucuuauaaga gucgaaaauu ggauauuuag aaacccagga      480

uuugcauuag ccgcagcagc aauagcaugg uuauuaggga guucaacauc acagaaaguu      540

auauauuuag uaaugauauu auuaauugca ccagcauacu caauaagaug uauaggaguu      600

ucgaauaggg auuucguaga aggaaugucg ggagguaccu ggguagauau aguuuuagaa      660

cauggcgguu guguaacagu uauggcacaa gauaaaccua caguagauau agaacuugua      720

acaacaacag uuagcaacau ggcugaagua agaagcuauu guuacgaagc aucaauauca      780

gacauggcau ccgauucaag augcccgaca caaggagaag cauaucuaga uaaacaauca      840

gauacacaau auguaugcaa acgaacucua guagacagag gcuggggaaa cgguugcgga      900

cuauucggaa aagguagccu aguaaccugu gcuaaauucg cauguucaaa gaaaaugacu      960

ggaaaaucaa uucaaccuga aaacuuagaa uauagaauua uguuaagcgu gcauggaagc     1020

caacauucag guaugauugu caaugauacg ggacaugaaa cagaugaaaa ccgugcaaaa     1080

guagaaauaa caccaaauuc cccgagggca gaagcaacac uagggggauu uggaucacua     1140

ggcuuagauu gugaaccuag aacuggauua gauuuuagug aucuauauua ccuaacaaug     1200

aacaauaaac auugguuagu ucauaaagaa ugguuucaug auauuccauu accguggcac     1260

gccggugcag auacagguac gccacacugg aauaacaaag aagcauuagu agaauuuaaa     1320

gaugcacaug cuaaacguca aacugucgua guauuagguu cacaagaagg agcaguacau     1380

acagcauuag caggagcccu agaagcggaa auggauggag caaaaggacg ccuaucaagu     1440

ggacacuuaa aaugucgcuu aaaaauggac aagcuaagau uaaaaggagu caguuauucu     1500

cuaugcacag ccgcuuucac auucacgaaa aucccugcgg aaacacuuca cggaacagua     1560

acuguagaag uucaauacgc agguacagac ggcccgugua aaguaccagc ccaaauggcc     1620

guagauaugc aaacauuaac accaguagga agacuaauaa cugcaaaucc aguuauaaca     1680

gaaucgacag aaaacagcaa aaugauguua gaacuagacc caccauuugg agauucuuac     1740

aucguuaucg gaguaggaga aaagaaaauu acacaccacu ggcauagaag uggaucaaca     1800

auuggaaaag cauucgaagc aacgguaaga ggagccaaac gaauggcagu uuuaggagac     1860

acugcauggg acuucggaag cguaggaggu gcauuaaacu cguuagggaa aggcauucau     1920

cagauauuug gagcagcauu caaaucauua uucggaggua ugucaugguu uagucaaaua     1980

cuaauuggaa cuuuacuaau gugguugggu uuaaacacua aaaacgguag uauaucacuu     2040

auguguuuag ccuuaggcgg aguguuaaua uuuuuaucaa cagcaguaag cgcugaugua     2100

gguuguugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4727
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4727
gggagaaagc uuaccaugag aggagcugau acaucaguag gaauuguagg uuuauuacua       60

acaacagcaa uggcagcaga aguuacaaga agaggaucag ccuauuauau guauuuagau      120

cgaaacgaug ccggagaagc aauaaguuuu ccaacaacuu uaggaaugaa uaaaugcuau      180

auacaaauca uggaucuugg acacaugugu gacgcaacaa uguccuacga auguccaaug      240

uuagacgaag gaguagaacc ugacgacguc gauuguuggu guaauaccac aucaacaugg      300

guaguuuacg guacaugcca ucauaagaaa ggagaagcaa ggcgaucaag gagagcagua      360

acauuacccu cacauaguac aagaaaauua cagacuagau cacaaacaug gcuugaaucg      420

agagaauaca cuaaacaccu aauaagagua gaaaacugga uuuuuagaaa uccaggauuu      480

gccuuagcag cagcagcgau agcauggcua cuugggucua guacaaguca aaaaguaauu      540

uacuuaguaa ugaucuuacu aauugcacca gcauacucaa uaagaugcau uggaguauca      600

aauagagacu uugucgaagg uaugagcggu ggaacuuggg uagacauugu cuuggagcac      660

gguggaugcg uaacuguaau ggcacaagac aaaccaacag uggacauaga auugguaaca      720

acaaccguaa guaauauggc agaaguuaga uccuauugcu acgaggcauc aauaagugac      780

auggcaagug auucaagaug uccaacacaa ggagaggcuu aucucgauaa acaaagugau      840

acccaauaug uauguaaaag aacauuaguu gacagagguu ggggaaaugg cuguggacua      900

uucgguaaag ggagcuuagu aacaugugca aaauuugcau guucaaagaa aaugacagga      960

aaaucuauac aaccagaaaa ucuagaauau aggauuaugu uaagcguuca uggaucgcaa     1020

cauaguggaa ugauugucaa ugacacagga caugaaacug acgaaaaccg agcuaaagug     1080

gaaauaacac caaauucacc acgagcagaa gcuacacucg gaggcuucgg aucacuagga     1140

uuggacugug aaccaagaac agguuuagau uucagcgauc uauauuauuu aacuaugaau     1200

aauaaacauu gguuagugca uaaagaaugg uuucaugaua uaccauuacc uuggcacgca     1260

ggagcagaua caggcacacc acacuggaau aauaaagaag cccuaguaga auuuaaagau     1320

gcacacgcua aaagacaaac aguaguugua cuaggaucac aagaaggugc uguacauaca     1380

gcacuugccg gggcauuaga agcagaaaug gauggagcua aaggaagauu aagcucaggu     1440

cacuuaaaau guagauuaaa aauggauaaa uuaaggcuaa aaggaguaag cuacucuuua     1500

uguaccgcag cuuucacauu uacaaaaaua ccagcagaaa cauuacaugg aacaguaacu     1560

guagaaguac aauaugcagg uacagauggu ccauguaaag uaccagcaca gauggcagua     1620

gauaugcaaa cauuaacacc aguagggaga uugauaacag cuaauccugu cauaacagaa     1680

ucaacugaaa auagcaaaau gaugcuagaa cuagauccac cauucgguga uucauauaua     1740

guaauaggag uuggagaaaa gaaaauaacg caucacuggc auagaagcgg uaguacaaua     1800

ggaaaagcau uugaggcaac aguaaggggc gcaaaaagaa uggcuguauu aggagauaca     1860

gcaugggauu ucggaagcgu cgguggagca cuaaauucac uaggaaaagg uauucaucaa     1920

auauucggag cagcuuuuaa aucuuuguuu ggaggaauga guugguuuuc ucaaauacua     1980

auuggaacau ugcugaugug guuaggacua aacacuaaaa acggaucaau uagcuuaaug     2040

ugucuagcuc ucggaggugu auugauauuu uuaucaacag cuguaucagc ggacguugga     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4728
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4728
gggagaaagc uuaccauggg agcugauaca agugucggaa uagucggacu auuacuaacu       60

acagcuaugg cagcggaagu uaccagaaga ggcagcgcau auuauaugua uuuagauaga      120

aaugaugcag gagaagcaau auccuuuccu accacucuag gaaugaauaa auguuauaua      180

caaauaaugg accuaggaca caugugcgau gccacuauga guuacgaaug cccuaugcuu      240

gaugaaggag ucgaaccuga ugauguagau ugcuggugua auacaacaag uacaugggua      300

guauacggaa caugccauca uaagaaagga gaggccagaa gaucaaggag agcaguaaca      360

cuacccucuc auucaacaag aaaauuacaa acaagaagcc aaacaugguu ggaaaguaga      420

gaauauacaa aacaucuaau ucgaguagaa aauuggauau uuaggaaccc aggauuugca      480

cuagcagcag cagcaauugc uugguuacua ggaucaucaa caucacaaaa aguaauauac      540

cuaguaauga uacuauuaau agcaccagca uauaguauaa gauguauagg aguaucuaac      600

cgagauuuug uugaaggaau gagcggggga acauggguag auauaguguu agaacaugga      660

ggauguguua ccguaauggc acaagauaaa cccacagugg acauagagcu aguuacaacu      720

accgucucaa auauggcaga gguacgaagc uauugcuaug aagcgagcau aagugauaug      780

gcaagugaua gccgaugucc uacccaagga gaagcauauc uagauaaaca aucagacacu      840

caauauguau gcaaacgaac auuaguugac agaggauggg ggaauggaug uggacuauuu      900

gggaaaggau cucuaguaac augugcaaaa uuugcuugua gcaagaaaau gacagguaaa      960

ucaauacaac cagaaaauuu agaguaucga auuaugcuaa gugugcaugg aucacaacau     1020

ucggguauga uaguaaauga cacuggacau gaaacugacg aaaauagagc gaaagucgaa     1080

auaacaccaa auuccccaag agcggaagcu acucuaggag gcuucggaag ucuggguuua     1140

gauugugaac cuagaaccgg acuagauuuc ucugauuuau auuauuuaac aaugaauaau     1200

aaacauuggu uaguacauaa agagugguuu caugauauuc cacuaccaug gcaugcuggu     1260

gcggauacag gaacuccaca uuggaacaau aaagaagcau uaguugaauu uaaagacgca     1320

caugcuaaac gacaaacagu cguaguacua ggaagucaag aaggagcugu ucacacagcu     1380

cuagcaggag cauuagaagc agaaauggau ggagcaaaag gaagauuauc aaguggacau     1440

cuaaaaugua gauuaaaaau ggacaaacuu agauuaaaag gaguaucuua uucucuaugu     1500

acagcugccu uuacauuuac aaaaauaccu gcugagacau uacacggaac aguuacaguu     1560

gaaguucaau augcaggcac ugauggacca uguaaggucc cagcucaaau ggcaguagau     1620

augcaaacau uaacaccagu uggaagauua auaaccgcaa auccuguaau aacagaaagc     1680

acagaaaauu caaaaaugau guuagagcua gauccaccau uuggagacuc auacauagua     1740

auaggaguag gugaaaagaa aauuacacau cauuggcaua gaucaggaag uacaauaggu     1800

aaagcauuug aagcaacagu aagaggagca aaaagaaugg caguccuugg cgauacugca     1860

ugggauuuug gaucaguugg aggagcacua aacucucuag gcaagggaau acaucaaaua     1920

uuuggagcag cuuucaaaag uuuauuugga ggaaugucau gguuuucuca aauacuaaua     1980

ggaacauuac uuaugugguu aggacuaaau acuaaaaacg gcaguauauc ucuaaugugu     2040

uuagcauuag gagguguuuu aauauuucuu ucuacagccg uaucggcaga uguuggaugu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4729
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4729
gggagaaagc uuaccauggc ugacacauca guuggaauag uaggacuauu auuaacaacc       60

gcaauggcag cagaaguaac cagaagagga ucagccuauu auauguaucu cgauaggaac      120

gacgcaggcg aagcaauauc auuuccuaca acacuaggua ugaauaaaug uuauauucaa      180

auaauggauc ugggacauau gugcgacgca accauguccu augaaugucc uauguuagau      240

gaagguguag agccagauga uguugacugu ugguguaaua caacuaguac uuggguagua      300

uacggaacau gucaucauaa aaaaggagag gcccgaagau caaggagagc cguaaccuua      360

ccaagucauu caacaagaaa auuacaaacu aggagucaga cuuggcuaga aagucgagaa      420

uauaccaaac auuuaauacg aguagaaaau uggauuuuuc gaaauccagg auuugcacuu      480

gcugcugcug cgauugccug guuacuugga agcucaacaa gucaaaaagu aauuuauuua      540

guuaugauau uauuaauagc accagcuuac agcauuagau guauaggagu gucaaauaga      600

gacuuuguag agggcaugag uggagguacg uggguagaua uaguauuaga acauggagga      660

ugcgugacag uaauggcaca agacaaacca acgguagaua uagaauuagu aacaacuacc      720

guaucgaaua uggcagaagu acgaucauau ugcuaugaag caucaauaag ugauauggcu      780

uccgacagca gaugcccuac ucaaggagaa gcauacuuag auaagcaaag ugauacccaa      840

uauguuugca aacgcacacu aguagauaga ggauggggua auggaugugg uuuauuuggc      900

aaaggaaguc ugguaacaug ugcuaaauuu gcaugcucaa aaaaaaugac aggaaaauca      960

auacaacccg aaaauuuaga auauagaaua auguuaagug ugcaugguuc acaacauagc     1020

ggaaugauug uaaaugauac uggacaugaa acagaugaaa auagagcaaa aguagaaaua     1080

acaccaaaca guccaagggc ggaagccaca cuagguggau uugguucauu gggacuagac     1140

ugugaaccgc gaacuggcuu agauuuuagc gauuuauauu auuuaacaau gaauaacaaa     1200

cauugguuag uacacaaaga augguuccau gacauuccac uuccauggca cgcuggcgca     1260

gauacuggaa caccucacug gaauaacaaa gaagcacuag uggaauucaa ggacgcacac     1320

gcaaaaagac aaacaguagu aguucuagga ucacaagaag gagcaguaca uacagcuuua     1380

gcgggagcau uagaagcaga aauggaugga gcaaaaggaa gacuaucaag cggacaucua     1440

aaauguaggu uaaaaaugga caaacuaaga uuaaaaggag ugucauauag uuuauguaca     1500

gcagcauuca cuuuuacaaa aauuccagcu gaaacauuac augguacagu aacaguugaa     1560

guucaauaug cuggaacaga uggaccaugu aaaguaccag cacaaauggc aguagacaug     1620

caaacacuaa cacccguugg ccggcuaauc acagcaaauc caguaauuac agaaucaacu     1680

gaaaauucaa aaaugauguu agaauuagau ccacccuuug gagauaguua cauaguaaua     1740

ggcguaggag aaaaaaaaau uacacaucau uggcauagau cugggucaac aauuggaaaa     1800

gcguuugaag caacaguaag gggcgcuaaa cguauggcag uucuaggaga uacggcuugg     1860

gauuucggcu caguaggagg agcacuaaau ucauuaggaa aaggaauaca ucaaauuuuu     1920

ggagcagcau ucaaaucauu auuuggagga augagcuggu ucucacaaau uuuaauaggc     1980

acauugcuaa uguggcuagg auuaaauacc aaaaaugguu caauauccuu aauguguuua     2040

gcauuaggag gaguuuuaau auuuuuaagu acagcaguau cagcagacgu uggauguuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4730
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4730
gggagaaagc uuaccaugga uacaucaguu gguauaguag gacuauuguu aacaacagca       60

auggcggcag aaguaacaag aagaggauca gcguacuaca uguaucuaga uagaaacgau      120

gcaggugaag caauaucauu cccaacgacc cuaggcauga acaaauguua uauacaaauc      180

auggaucuag gucauaugug ugaugcaaca augucuuaug aaugucccau guuagaugaa      240

ggcguagagc cugaugacgu agacuguugg uguaauacaa caaguacaug gguaguuuau      300

ggaacauguc aucauaaaaa aggagaagca agaagaagca gacgagcugu gacacuaccu      360

ucacacagua caagaaaauu acaaacaaga ucacaaaccu gguuggaaag cagagaauau      420

acaaaacacu uaauacgagu agagaacugg auuuuuagaa auccagguuu cgcauuagcu      480

gcagcagcaa uagcauggcu auuagguagu ucaacaucuc aaaaaguaau auauuuggua      540

augauauuac uaauagcucc agcuuauagu aucagaugua ucggcguauc aaauagagac      600

uuuguugaag guaugucagg aggaacaugg gucgauauag uucuugaaca ugguggaugu      660

guaacaguaa uggcacaaga caaaccuaca guagauauag aauuaguaac cacaacagua      720

ucaaacaugg cugaaguaag aucuuauugu uaugaagcaa gcauaucaga uauggcaagc      780

gacagcagau gcccaacaca aggagaagcu uaccuagaca aacaaaguga uacucaguac      840

guauguaaac ggacacuugu ggauagaggu uggggaaacg gauguggauu auuuggaaaa      900

ggcaguuuag uaacaugcgc aaaauuugcc uguucaaaaa aaaugaccgg aaaaucaaua      960

cagccagaaa aucuagaaua ccguauaaug uuaaguguac augguaguca acauaguggg     1020

augaucguaa augauacagg acaugaaaca gacgaaaaca gagcgaaagu agaaauuaca     1080

ccuaauucac caagagcaga agcaacauua gguggauuug gcucacuugg auuagauugc     1140

gaaccaagaa cuggacuaga uuuuucagau uuauacuauu uaacaaugaa uaauaaacau     1200

ugguuaguuc acaaagaaug guuucaugau auaccucuac cuuggcacgc gggagcagac     1260

acaggaacac cucacuggaa uaauaaagaa gcuuuaguug aauuuaaaga ugcucacgca     1320

aaacgacaaa cuguaguugu cuuagguucu caagaaggag caguacauac ugcauuagca     1380

ggcgcucuag aggcagagau ggauggagca aaagguagau uaucgagugg acauuugaaa     1440

ugcagauuaa aaauggacaa auuaagguua aaaggaguaa guuacucauu auguacagca     1500

gcauuuacau uuacaaaaau accagcagaa acauuacacg gaacuguaac cguugaagua     1560

caauaugcag guacagaugg accaugcaaa guaccagcac aaauggccgu agacaugcag     1620

acauugacuc caguaggaag auuaauaaca gcaaauccag uaauaacuga aagcacggaa     1680

aauaguaaaa ugaugcucga auuagaucca ccauuuggag auaguuacau cguaaucgga     1740

guuggcgaaa aaaaaauuac acaccacugg cauagaucug guucaacaau aggaaaagca     1800

uucgaagcaa caguacgcgg agccaaacga auggcaguuu uaggagauac agccugggau     1860

uuuggcagug uaggaggcgc ucucaauagu cuuggaaaag gaauacauca aaucuuugga     1920

gcagccuuua agagucuguu cggaggaaug aguugguucu cacaaauacu aauagguacg     1980

uuauuaaugu ggcuaggauu aaacacaaag aaugguucaa uaucuuugau gugucuagcu     2040

uuaggaggcg uacucaucuu uuugucaaca gcaguaucag cugauguagg augcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4731
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4731
gggagaaagc uuaccaugac aaguguagga auuguaggau uacuacuaac aacagcuaug       60

gcagcagaag uaacaagaag gggcucagca uauuauaugu auuuagauag aaacgaugcu      120

ggggaagcaa uaagcuuccc uacaacauua ggaaugaaca aauguuacau acaaauuaug      180

gacuuagggc auauguguga ugccacaaug ucuuaugaau gcccaauguu agaugaagga      240

guagagccug acgauguaga cuguuggugu aauacaacau caacuugggu gguauauggc      300

acaugucauc auaaaaaagg cgaagcacga agauccagga gagcuguaac acuaccauca      360

cauaguacaa gaaaauuaca aacaaggucu caaacauggu uagaaucucg cgaauauacc      420

aaacaccuua uaagaguaga aaauuggaua uucagaaauc caggcuucgc acuagcagcu      480

gcagcuauag caugguuauu aggaucgucc acaucacaaa aagucauaua uuuaguaaug      540

auacuauuaa ucgcaccagc auauucaaua agguguauag gagugucaaa cagagauuuu      600

guugaaggaa uguccggagg aacaugggug gacauaguuc uugaacacgg aggauguguu      660

acaguuaugg cucaagauaa accaaccguu gacauagaau uaguaacaac aacuguaagu      720

aauauggcag aaguaagaag uuacuguuau gaagcaagca uaucagauau ggcaucagac      780

ucaagaugcc caacacaagg ugaagcauau uuggauaaac aaucagauac acaauaugua      840

uguaaaagaa cacuaguaga uagagguugg gggaacggau guggauuauu uggaaaaggc      900

agcuuaguaa caugugcaaa auuugcaugc agcaaaaaaa ugacaggaaa aucuauacaa      960

ccagaaaauc uagaauacag aauaauguug ucaguacaug ggucacaaca uucaggaaug     1020

auaguaaacg auaccgggca cgaaacagac gagaaccgag cgaaaguuga aauaacacca     1080

aacagcccaa gagcugaagc aacauuaggg ggauucggcu cauuaggacu agauugcgag     1140

ccaagaacag gauuagacuu uucagaucug uauuauuuaa caaugaacaa uaaacauugg     1200

uuaguucaua aagaaugguu ucacgauauu ccauuaccau ggcaugcggg agcagauacc     1260

ggaacaccac auuggaauaa caaagaagcu uuaguagaau ucaaagacgc acaugcaaaa     1320

agacaaacag uuguaguauu agguagccag gaaggggccg uacauacagc acuugcagga     1380

gcauuagagg cagaaaugga uggugcuaaa ggcagacuaa guaguggaca ucuaaaaugu     1440

aggcuaaaaa uggauaaauu gagacucaaa ggagucucau auagucuaug uaccgcagca     1500

uucacauuua caaaaauacc ugcagaaaca cuucauggaa caguaacugu ugaaguacaa     1560

uacgcaggga cugauggacc auguaaaguc ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccug uugguagguu aauaacagca aacccuguga uaacugaauc uacagaaaac     1680

agcaaaauga uguuagaguu agacccacca uuuggagaua guuauauagu cauaggagua     1740

ggcgaaaaaa aaauaacuca ucauuggcac agaucaggca guacgauagg uaaagcauuu     1800

gaggcaacag uacgcggagc gaaacgaaug gcaguacuag gagauacagc augggauuuc     1860

ggcucaguug gaggagcauu aaauucacuu ggaaaaggca uacaccaaau auuuggugca     1920

gcauucaaaa gccuauuugg aggaauguca ugguuuaguc aaauauuaau aggaacauug     1980

cuaauguggc uuggauuaaa uacaaaaaau ggauccauau cacuaaugug ccuagcauua     2040

ggaggaguau uaauauuccu aucuacggca gugagcgccg auguaggaug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4732
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4732
gggagaaagc uuaccaugac uagcguagga auagucggac uucuauugac aaccgcuaug       60

gcagcagcag uuacaagaag aggcuccgca uauuacaugu accuagacag aaaugaugcc      120

ggagaagcca uaagcuuucc aacaacacua ggaaugaaua aauguuacau ucaaaucaug      180

gaucucggac auauguguga ugcaacaaug aguuaugaau guccaauguu agacgaagga      240

guagaaccug augaugucga uugcuggugc aauacaacau caacaugggu cguauauggc      300

acuugccacc auaaaaaggg agaagcuagg cguucacgaa gagcaguaac acuaccaucc      360

cauucaacua gaaaauuaca aacccgaagu caaacuuggu uagaauccag agaauauaca      420

aaacacuuga uaagaguaga aaauuggaua uuuagaaauc cagguuucgc cuuagcagcu      480

gcagcaauug caugguuacu aggaagcuca acaucacaaa aaguuauaua cuuaguaaug      540

auacuauuaa uagcuccagc uuacucaaua agauguauag gaguuucaaa uagggauuuc      600

guagaaggga ugucaggagg uacauggguc gacauaguau uagaacaugg aggaugcgua      660

acaguuaugg cucaagauaa accaacgguc gauaucgaac uuguuacaac uacaguaucu      720

aauauggcag aaguaagaag uuauuguuau gaggcaucua uaagugauau ggcuagugau      780

aguagauguc caacacaagg ugaagcauac uuagauaaac aaucugacac acaauauguu      840

uguaaaagaa cacuaguaga ccgaggaugg ggcaacgggu guggauuauu uggaaaagga      900

aguuuaguaa caugcgcaaa auuugcaugu ucaaaaaaaa ugacaggaaa aucgauucaa      960

ccugaaaauc uagaauauag aaucaugcuu ucaguucacg gaucacagca uaguggaaug     1020

auaguaaaug auacagguca cgaaacggac gaaaauagag ccaaaguaga aauaacuccg     1080

aauucaccaa gagcggaagc cacucuuggc ggauuuggau cauuaggauu agauugugaa     1140

ccaaggacag gacuagauuu cucagaucua uauuaccuua caaugaauaa caaacauugg     1200

uuaguacaca aagaaugguu ucaugauaua ccacuaccuu ggcacgcagg agcugauacu     1260

gguacaccac acuggaacaa caaagaagcg uuaguagaau uuaaagaugc acaugcaaaa     1320

agacaaaccg uaguaguacu cggaagccaa gaaggagcag uacauacagc ucuagcugga     1380

gcauuagaag cagaaaugga cggagcaaaa ggaagauuau cuaguggcca ccuuaaaugu     1440

agauuaaaaa uggauaaguu aagacucaaa gguguaucau acucauuaug uacugcugca     1500

uuuacauuca cgaaaauacc ugcagaaaca cuacauggaa cuguaacugu ugaaguccaa     1560

uaugcaggua cagauggacc auguaaagua ccagcacaaa uggcugucga uaugcaaacg     1620

uuaacuccug uaggacgauu aauaacagca aauccaguua uaacggaauc cacagaaaac     1680

ucaaaaauga uguuagaauu agauccacca uuuggagauu cauauauagu uaucgguguu     1740

ggagaaaaaa aaauaacuca ucauuggcau agaagugguu caaccauagg aaaagcauuu     1800

gaggcaacug uaagaggagc aaaaagaaug gcugugcugg gagauacagc uugggauuuu     1860

ggaagugugg gcggagcauu aaauagcuua gguaaaggua uacaucaaau auuuggagca     1920

gcauuuaaau cacuauuugg uggaauguca ugguuuaguc aaauacugau agguacucuu     1980

uugauguggu uaggauuaaa uacaaaaaau ggaagcauaa guuuaaugug uuuagcauua     2040

ggaggaguau uaauauuucu aagcacagca guaucagcug acguaggaug uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4733
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4733
gggagaaagc uuaccaugag aggagcagau accaguguag gaauaguagg ucuauuauua       60

acaacagcua uggcagcagc cguaaccaga agaggaagug cauauuauau guaccuugac      120

aggaaugaug cgggugaagc aauuaguuuu ccaacaacac uugggaugaa uaaauguuau      180

auucaaauua uggaucuugg ucauaugugu gaugcaacaa ugagcuacga auguccuaug      240

cucgaugaag gaguagaacc agaugaugua gauuguuggu guaauacaac gagcacuugg      300

guaguauaug gaaccugcca ucauaagaaa ggggaagcua gaagaaguag gagagcagua      360

acacugccau cacauucgac aagaaaacua cagacaagaa gucaaacaug guuggaaagu      420

agagaauaca caaaacauuu aauuagagua gaaaacugga uauuuagaaa uccuggauuc      480

gcauuagcag cagccgccau agcuugguug uuaggaagua gcacaaguca aaaagugaua      540

uauuuaguua ugauauuacu aauagcuccu gcauauagua uaagaugcau aggaguaagu      600

aauagagacu uuguugaagg uauguccggg ggaacauggg uagacauagu acuagaacau      660

ggaggaugug uuacaguuau ggcacaagau aaaccuacag uggauauuga acuuguaacu      720

acaacggucu caaauauggc agaaguacga ucauauuguu acgaagcaag uauaucugau      780

auggcaucug acucaagaug cccaacgcaa ggagaagcau accuugauaa acaaagugau      840

acacaauacg uaugcaaaag aacucuagua gacagaggau ggggaaaugg cuguggauua      900

uucggcaaag gaucucuugu uacaugcgca aaauuugccu gcucgaagaa aaugacagga      960

aaaaguauuc aaccagaaaa uuuagaauau agaauaaugu uaagugugca cgguucacaa     1020

cauuccggua ugaucguaaa cgauacagga caugaaacag augaaaauag agcuaaagua     1080

gagauuacac caaauagucc acgagccgaa gcaacacucg gcggauuugg caguuuaggu     1140

uuagauugcg aaccgagaac agguuuagau uuuucagauu uauauuauuu aaccaugaac     1200

aauaaacauu gguuagucca uaaagaaugg uuucacgaca uaccucuacc auggcaugca     1260

ggagcagaua cagguacccc ucauuggaau aauaaagagg cuuuaguaga auuuaaagau     1320

gcacacgcaa aaagacaaac agugguugua uuagguagcc aagaaggugc aguacauaca     1380

gcucuagcag gagcauuaga agcggaaaug gauggugcaa aaggaagauu aaguucaggg     1440

cacuuaaaau guagauuaaa gauggacaag uuacgauuaa aaggaguauc auauagccua     1500

uguacagcgg cauuuacauu uacuaaaaua ccagcagaaa cacuacacgg aacaguaaca     1560

guugaaguac aauaugcugg aacagauggc cccuguaaag uaccagcaca aauggcagua     1620

gacaugcaaa cacuaacccc aguaggaaga uuaauaacag caaauccggu aauuacugaa     1680

ucuacugaaa auagcaaaau gaugcuagaa uuagauccac cauucggcga cagcuauaua     1740

gucauagggg ugggagaaaa gaaaauuaca caucauuggc acagaucagg aaguacuauc     1800

gguaaagcau uugaagcaac aguaagagga gcuaaaagaa uggcaguacu aggggauaca     1860

gcaugggacu uuggaagugu cgguggagca cuaaauucuu ugggaaaagg gauacaccaa     1920

auauuuggug cagcauuuaa aaguuuauuc gguggaaugu cuugguucag ucagauacua     1980

auuggcacuc uuuuaaugug guuaggucua aauacgaaaa augggucaau aucucuaaug     2040

ugucuagcau uaggaggugu uuuaaucuuc uuaagcacag caguaucagc agacguagga     2100

uguugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4734
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4734
gggagaaagc uuaccaugcg aggacuguca ucaagaaaaa gaagaucaca ugauguacuu       60

acaguacaau ucuuaauauu aggaauguua cuaaugacag gaggcguaac aagaagaggu      120

agcgccuacu auauguaucu agauagaaau gaugcaggag aagcaaucag uuucccuaca      180

acccuuggua ugaauaaaug cuauauacaa auaauggauu uggggcacau gugugacgcu      240

acaaugaguu acgaaugucc aauguuagau gaaggagucg aaccagauga uguagauugc      300

ugguguaaca ccacaucaac auggguggua uacgggacau gccaucauaa gaaaggagaa      360

gcaagacguu caagacgagc uguaacauua ccaagucaua gcacaagaaa auuacaaacu      420

agaucacaaa caugguuaga aucccgagag uacacaaaac aucuaauaag aguggaaaau      480

uggauuuuua gaaauccagg uuucgcauua gcugcagccg cuauagccug guuauuaggu      540

ucaucuacaa gucaaaaagu aauauauuua guaaugauau uacuaauagc accagcauau      600

ucuauaagau guauaggagu cucaaauaga gauuucguag aagguaugag cggaggaacu      660

uggguagaua uuguacuaga acaugguggc uguguaacag uaauggcuca agauaaaccu      720

acaguagaua uagaauuagu aacaacgaca guaucaaaua uggcagaagu uagaucuuau      780

uguuacgaag caagcaucuc agauauggca agugacagua gaugcccaac acaaggggaa      840

gcauaucuag acaaacaauc agacacucaa uauguaugua aaagaacuuu aguugaccga      900

ggauggggaa augguugugg acuauucggu aaaggaucau uaguuacuug ugcgaaauuc      960

gccugcagca agaaaaugac aggaaaauca auccaaccag aaaacuuaga auacagaaua     1020

augcuaagug uacauggaag ccagcacagu ggaaugauag uaaaugauac aggccaugaa     1080

acagaugaaa auagagcaaa aguagaaaua acaccaaauu caccgagagc ugaggcaaca     1140

cuaggaggau uugggagcuu aggauuagau ugugaaccaa gaacaggccu agacuuuagc     1200

gauuuauauu acuuaacaau gaauaauaaa cacuggcuag uacacaaaga augguuucau     1260

gacauaccuu uaccauggca ugcaggagca gacacuggua caccacauug gaacaauaaa     1320

gaagcauuag uagaauuuaa agacgcccau gcgaaaagac aaacgguagu aguacuagga     1380

agucaagaag gggcaguaca uacagcuuua gcuggugcau uagaagcuga aauggaugga     1440

gcaaaaggua gacuauccuc aggucacuua aaauguagac uaaaaaugga caaauuaagg     1500

uugaaaggag uaucauacag uuuauguaca gcagcauuua cauuuacuaa aauaccugca     1560

gagacacugc auggaaccgu uacaguggaa guacaauacg caggaacaga uggaccaugu     1620

aaaguaccag cacaaauggc gguagauaug caaacacuaa ccccgguagg uagauuaaua     1680

acagcaaacc cugugauaac agaaagcacu gaaaacucaa agaugauguu agaauuagac     1740

ccaccauucg gugauaguua uauaguaaua ggagucggag aaaagaaaau aacacaucac     1800

uggcauagaa gugguucaac aauagguaaa gcguucgaag caacaguucg aggagcaaaa     1860

agaauggccg uacuaggaga uacugcaugg gacuuuggau caguaggagg agcuuuaaac     1920

ucucuaggua aagguauuca ucaaauauuc ggggcugcuu uuaagagcuu auuuggcgga     1980

augucuuggu uuucacaaau auuaauagga acacuauuaa uguggcuagg acuaaauacg     2040

aaaaauggaa gcauaagucu gauguguuua gcacugggag gaguacucau cuuccuaagc     2100

acagcaguuu cagccgaugu aggauguuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4735
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4735
gggagaaagc uuaccaugcg aggacuauca ucaagaaaaa gaagaucaca ugacguccuu       60

acaguacaau uuuuaauauu aggaauguua uuaaugaccg gagcaguaac uagacgagga      120

ucagcauauu acauguaccu agaccgaaau gacgcaggag aagcuauauc auuuccuaca      180

acccuaggaa ugaacaaaug uuacauacaa auaauggauu uaggacauau gugugaugcc      240

acaaugucau augaaugucc aaugcucgau gaaggaguag aaccggauga cgucgauugu      300

uggugcaaca caacuucgac uuggguagua uacgguacuu gucaucauaa gaaaggagaa      360

gcgagacguu caaggagagc agugacacua ccgucucacu caacaagaaa auuacaaaca      420

agaucacaaa cgugguuaga aagcagagaa uauacaaaac acuuaaucag aguggaaaau      480

uggauauuua ggaacccagg cuucgcuuua gcagcggcag caauagcaug guuacuagga      540

uccagcacau cacaaaaagu aauauacuug guaaugauau uacuaauugc uccagcauac      600

ucaauuagau guauaggugu aaguaacaga gauuucguug aaggaaugag uggaggaaca      660

ugggucgaua uaguacuuga gcauggcgga ugcguaaccg uaauggcaca agauaagcca      720

acuguagaua uagaacuggu uacaacaaca gucucuaaua uggcagaagu aagaagcuau      780

uguuaugaag cgucaauauc agauauggcg ucagacucaa gaugccccac ccaaggagaa      840

gcauaucuag auaaacaaag ugacacacaa uauguaugca aaaggacucu aguagauaga      900

ggauggggaa augggugugg auuauucgga aaaggguccc uaguaacaug cgcaaaauuu      960

gccugcucaa agaaaaugac agggaaauca auucaaccag aaaaccuaga auacagaaua     1020

auguuaagcg uucauggaag ucaacauucg gguaugauug uaaacgauac aggacaugaa     1080

acugacgaaa auagagcgaa aguugaaaua acaccuaauu caccaagagc agaagcaacu     1140

cucggaggau ucggaagucu uggacuggau ugcgaaccac gaacaggguu agauuuuagc     1200

gaccuauauu aucuaacgau gaauaacaaa cauugguuag uacacaaaga augguuucau     1260

gacaucccac uaccauggca ugcaggagca gacacaggua cgccacauug gaauaacaaa     1320

gaagccuuag uagaauuuaa ggaugcacau gcaaaaaggc aaacuguagu ugucuuagga     1380

agccaagaag gcgcaguaca uacagcauua gcuggagcuu uagaggcuga aauggaugga     1440

gcaaaaggua gguuaucaag uggacauuua aaaugucgau uaaaaaugga uaaauuaagg     1500

uuaaaaggag uaucuuauuc auuaugcaca gcagcauuua cauuuacaaa aauaccagca     1560

gaaacccuac augguacagu aacaguagaa guccaauaug caggcacaga uggaccaugc     1620

aaaguuccag cucaaauggc cguagauaug caaacuuuaa cacccguugg aagacuaaua     1680

acagcaaauc caguaauaac agaaucaaca gaaaauagua aaaugaugcu agaacuagau     1740

cccccauuug gcgauagcua uauagucaua ggggucggag aaaagaaaau uacacaucac     1800

uggcaucgau caggcaguac aauagguaaa gcauuugaag caacaguacg aggagcuaaa     1860

agaauggcag uccuaggaga uacagcuugg gacuuugguu caguaggagg agcauuaaac     1920

ucauuaggaa aagguauaca ucaaauauuu ggagcagcuu ucaaaucauu auuuggaggc     1980

augucuuggu uuagucagau acuaauagga acucuauuaa ugugguuagg cuuaaauacu     2040

aaaaauggaa gcauaucuuu aaugugucua gcuuuaggug gaguauuaau auuuuuaagc     2100

acagcuguau cagcugaugu aggcuguuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4736
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4736
gggagaaagc uuaccaugag gauaauaaau gcccgcaaag aaaagaaacg gagaggagcc       60

gauacaucug uagguauugu aggauuauua uuaacaacag cgauggcagc agaaguaaca      120

agaagaggau cagcuuauua uauguauuua gacagaaacg augcaggaga agcaauuagc      180

uuuccaacua cauuagguau gaauaaaugu uauauacaaa uaauggauuu aggacauaug      240

ugugaugcga caauguccua ugaaugucca augcuagaug aagguguaga gccagaugau      300

guagauugcu gguguaauac aacaucaaca ugggucguau auggaacaug ccaucauaag      360

aaaggugaag caaggcguag uaggagagca guuacauuac caucucauuc uacuagaaaa      420

cuacaaacaa gaucacaaac augguuagaa agucgagaau auacuaaaca uuuaauaaga      480

guagaaaacu ggauuuuuag aaacccagga uuugcacuag cugcagcugc aauagcaugg      540

uuguugggaa gcaguacaag ucagaaagua auuuauuuag uaaugauauu auuaauugca      600

ccagcauauu caauuagaug uauaggugua ucuaacagag acuuuguaga gggcauguca      660

ggaggaacau ggguugauau uguuuuagaa caugguggau guguaacugu aauggcucaa      720

gauaaaccaa caguagacau cgaauuagug acuacaacag uaucaaacau ggcugagguu      780

agauccuauu guuaugaagc cucaaucagc gauauggcca gugauaguag auguccaaca      840

caaggugaag cauauuuaga uaaacaaagu gauacgcaau auguuuguaa aaggacguua      900

guagaucggg gcuggggaaa cggaugcggc uuauuuggaa aaggaaguuu aguaacaugu      960

gcaaaauuug caugcucaaa gaaaaugaca gguaaaagca uucagccuga aaauuuagaa     1020

uauagaauaa ugcuaagugu acauggcagu caacauucag gaaugauugu aaaugacacg     1080

ggacacgaaa cagacgaaaa cagagcaaaa gucgaaauaa cgccuaauag uccaagagca     1140

gaagcaacuu uagguggauu uggaagccuu ggacuagauu gugaaccacg aacugguuug     1200

gauuucagug auuuauacua uuuaacuaug aauaacaaac acugguuagu acauaaagaa     1260

ugguuucaug acaucccauu accauggcau gcaggagcgg auacugggac accacauugg     1320

aacaauaaag aggcguuagu cgaguucaaa gaugcacaug cgaaacgaca gacaguagua     1380

guauugggaa gucaagaagg agccguacac acagcauuag cuggagcauu agaagcugaa     1440

auggacggag caaaaggaag auuaucaucu ggacacuuga aauguagauu aaaaauggau     1500

aaacucagac ugaaaggagu aagcuacagu cuauguacag cagcauucac auuuacaaaa     1560

auuccugcag aaacacuaca uggaacaguu acaguagaag uacaauaugc aggaacagau     1620

ggaccaugua aaguaccggc acaaauggcu guagacaugc aaacacuuac gccuguagga     1680

agguuaauua cagcaaaccc ugugauaaca gaaaguacgg aaaauucaaa aaugauguua     1740

gaacuagacc cuccauucgg ugauaguuau auagucauag gaguagguga aaagaaaauu     1800

acacaccauu ggcaucgcuc aggcucaaca auaggaaaag cguuugaagc gacaguaagg     1860

ggagcaaaaa gaauggcagu guuaggggau acagcauggg auuucggauc gguaggaggu     1920

gcauugaaua gucuaggaaa agguauucac caaauauuug gagcagcguu caaaucauua     1980

uuugggggua ugaguugguu cucacaaaua uuaauaggga ccuuauuaau guggcuagga     2040

cuaaauacga aaaaugguuc aauuagucuc auguguuuag cauuaggugg aguauugaua     2100

uuccuaagua cagcaguauc cgcagauuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4737
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4737
gggagaaagc uuaccaugau aauuaacgca agaaaagaaa aaaagagaag gggggcagac       60

acaucuguag gaaucguagg guuauuauua acaacagcua uggcagccga aguaacgcga      120

agaggaucag cuuauuauau guauuuagac agaaacgaug caggagaagc aauaaguuuu      180

ccaacuacuc uaggaaugaa uaaauguuac auacaaauaa uggauuuagg acacaugugu      240

gacgcaacaa ugagcuauga augcccaaug uuggaugaag gcguagagcc cgaugaugug      300

gauuguuggu guaauacaac aagcacuugg gugguauaug gcacuugcca ucacaaaaaa      360

ggcgaagcaa gaaggucuag aagagcagua acauuaccau cccacucaac aagaaaauua      420

caaacaagau cccaaacaug gcuagaaucu agagaauaua caaaacauuu aauucgcgua      480

gaaaauugga uauucagaaa uccgggguuu gcacuagcag cagcugcaau agcauggcua      540

uuaggaucau cgacaagcca aaaaguaaua uauuuaguaa ugauacuacu cauagcaccu      600

gcauacucaa uaagauguau cgguguauca aauagagauu ucguggaagg caugaguggu      660

ggaacauggg uugacauagu uuuagaacau ggagguugug uuacuguaau ggcucaagau      720

aaaccaacag uagauauaga acucguaaca acaacaguuu caaauauggc ggaaguaaga      780

aguuauuguu augaagcuuc uauuucagau auggccagcg acagucguug cccaacacaa      840

ggugaagcau aucuagacaa gcaaagugau acucaauaug uaugcaaaag aacacuagua      900

gacagagguu gggguaaugg augugguuua uuuggaaaag gaagccuagu aacaugcgca      960

aaauuugcuu gcaguaaaaa aaugacaggg aaaaguauac agcccgaaaa ccucgaguau     1020

agaauaaugu uaucuguaca uggaucacaa cacagcggaa ugauaguaaa ugauacagga     1080

cacgaaacag augaaaacag agcaaaaguc gaaauaacac caaauagucc ucgagcugaa     1140

gcuacauuag gaggauucgg aucgcuaggg uuagauugcg aaccuagaac uggauuagac     1200

uuuucugauu uauauuaccu gacaaugaac aacaaacacu ggcuuguaca uaaagaaugg     1260

uuccaugaua uuccauuacc auggcaugcc ggagcagaua caggaacccc ccauuggaau     1320

aauaaagaag cauuaguaga auucaaagac gcgcacgcua aaagacaaac aguaguagua     1380

cuaggaucac aagaaggagc uguacauaca gcauuggcag gagccuuaga agccgaaaug     1440

gacggagcaa aaggucgucu uucaagcgga cauuugaaau guagacuaaa aauggauaaa     1500

cucagacuaa aagggguauc auauucacua uguacagcag cauuuacauu uacaaaaaua     1560

ccagcagaaa cauuacaugg uacagucaca guagaaguuc aauaugcagg aacagauggu     1620

ccguguaaag uaccugcaca aauggcagua gauaugcaga cauuaacacc gguugguagg     1680

uuaauuaccg caaauccagu aauaacagaa aguacagaaa auucaaaaau gauguuagaa     1740

uuagauccac cauuuggaga cagcuauaua guaauaggag uaggugaaaa aaaaauaaca     1800

caucauuggc auagaagugg aucaacaaua ggaaaagcau uugaagcaac ggucagagga     1860

gcaaaaagaa uggccguuuu aggggauaca gcaugggauu uuggaagugu agguggagca     1920

uuaaacucuu uagguaaagg cauacaucaa auauuuggag cggcuuuuaa aaguuuauuc     1980

ggugguaugu caugguuuag ucaaauacua auaggaacuu uauuaaugug guugggauua     2040

aauacaaaaa acgguucgau uaguuuaaug ugcuuagcau uaggcggagu auuaauauuc     2100

uuaaguacgg cgguaagugc ugauugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4738
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4738
gggagaaagc uuaccaugau aaaugcaagg aaagaaaaaa aaagaagagg agcagauaca       60

uccguuggaa uaguuggacu gcuauuaaca acagcuaugg cagcagaggu uacacgaaga      120

ggaagcgcau auuauaugua ucuagauaga aaugaugcag gagaagcuau aaguuuuccg      180

acaacauuag gaaugaauaa auguuauaua caaauaaugg accuaggaca uaugugugau      240

gcaacaauga guuaugaaug uccaauguug gacgaaggag uagaacccga cgacguagau      300

uguuggugua auacaacaag caccugggua guauauggca caugucacca uaagaaagga      360

gaagcaagaa gaucacgaag agcgguaaca uuaccaucac auucuacacg aaaauuacaa      420

acaaggucac aaacaugguu agaaagcaga gaauauacaa aacauuuaau ucgaguggaa      480

aauuggauau uuagaaaucc aggauuugca cuagccgcag cagcaauagc augguuacua      540

ggaucaucaa caucacaaaa aguaauauau uuaguaauga uacuacuuau agcaccagcc      600

uacucaauua gaugcauugg aguaucuaau agagauuucg uagaaggaau gagcggagga      660

acuugggugg auauuguauu agaacacgga ggauguguaa caguaauggc ucaagauaag      720

ccaacaguag acauagaauu aguaacaacu acaguaagua auauggccga ggucagaagc      780

uacugcuaug aagcaucaau uucagauaug gcaagcgauu caagaugucc aacacaaggu      840

gaagcuuauu uggacaaaca aagugacaca caauauguau gcaaacgaac acugguagau      900

cgcggauggg gaaauggaug uggauuauuu ggaaaagggu cauuaguaac augugcaaaa      960

uucgccuguu caaaaaaaau gacuggaaaa uccauucaac ccgaaaaucu agaauauaga     1020

auaauguuaa guguacaugg aucacaacau ucugguauga uaguaaauga uacaggucau     1080

gaaacagaug aaaaccgcgc aaaaguagaa auaacaccua acucaccuag agcagaagca     1140

acacuaggag gauuuggaag cuuaggguug gacugugaac cuagaacagg acuggacuuu     1200

ucugacuuau auuaucuaac aaugaacaau aaacauuggu uaguacauaa agaaugguuc     1260

caugauauac cauuaccaug gcaugcagga gcugauacag gaacaccuca cuggaacaau     1320

aaagaagcac ucguagaauu uaaagaugcc caugcgaaaa gacaaacugu aguaguuuua     1380

ggaucccaag aaggcgcugu acacacugca cuagcugggg cauuagaagc cgaaauggac     1440

ggugcaaagg gacgauuauc auccggacau uugaaauguc guuuaaaaau ggacaaauua     1500

agacuaaaag gaguaucgua uucacuaugu acagcagccu uuacuuuuac aaaaauacca     1560

gcagaaacau uacauggaac aguuacaguu gaaguacaau augccggaac agacggacca     1620

ugcaaaguuc cagcacaaau ggcuguagau augcaaacau uaacuccagu aggcagauug     1680

auaacagcaa acccugucau aacagaauca acugaaaaca guaaaaugau gcuugaacua     1740

gaccccccau uuggagacuc auacauagua auagggguag gugaaaaaaa aauaacacac     1800

cauuggcaua gaaguggauc cacaauaggu aaagccuuug aagcaacugu acguggagca     1860

aaaagaaugg cagucuuagg ggauacagca ugggauuuug gaucaguagg aggagcuuua     1920

aacucuuuag gaaaaggaau acaucaaaua uuuggagcag cauucaaauc acuuuuugga     1980

ggaaugucau gguuuucuca aauucuaauc gggacacuac uuauguggcu aggauuaaau     2040

accaaaaacg gcaguauauc acugaugugu uuggcauuag guggaguuuu aauauuuuug     2100

agcacagcgg ugucagcaga uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4739
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4739
gggagaaagc uuaccaugaa ugcgaggaaa gaaaagaaaa gaagaggugc agauacauca       60

gucggaauag uaggacuguu auugacaaca gcgauggcag cagaaguaac aagaagagga      120

agcgcauauu auauguacuu agauagaaac gaugcuggug aagcaauauc auuuccuaca      180

acauuaggaa ugaacaaaug uuauauacaa auaauggauu ugggacauau gugugaugcu      240

acaaugaguu augaaugucc aauguuggau gaaggaguag agccagacga uguagauugu      300

ugguguaaca caacaucaac auggguagua uaugggacuu gucaccauaa gaaaggggaa      360

gcucgacgaa gccguagagc gguaacgcua ccaagccaua gcacaagaaa auuacaaacg      420

agaagucaaa cuugguuaga aagcagagaa uacacaaaac auuuaauaag aguagaaaau      480

uggauauuca gaaacccagg guuugcacua gcggcggcug caauagcaug gcuccuagga      540

ucaucaacau cacagaaagu aauauaccua guaaugauac uacuaauagc uccugcauau      600

aguaucagau guauaggagu aaguaaucga gauuuuguag aagguauguc cgguggaacg      660

uggguagaca uaguacuaga acauggagga uguguaacag ucauggcaca agacaaaccg      720

acaguagaca uagaauuagu uacgacaaca guaucaaaua uggcagaagu gagaucuuau      780

uguuacgaag caagcauaag cgacauggca agugacagcc guuguccaac acaaggagaa      840

gcauacuuag auaaacaaag ugauacacaa uauguaugua aaagaacgcu aguugacagg      900

ggcuggggaa acggaugcgg cuuauucgga aaaggaucuc uaguuacaug ugcaaaauuu      960

gcauguucaa agaaaaugac aggcaaauca auacaaccgg aaaauuuaga auauaggaua     1020

auguuaucag ugcaugguuc acaacauuca ggaaugauag uuaacgauac cggucaugag     1080

acagacgaaa auagagcaaa aguagaaaua acacccaacu caccaagagc agaagcaaca     1140

cuagguggau ucgguagcuu aggauuagac ugugaaccaa ggacaggauu ggauuuuagu     1200

gaccuauauu accuaacaau gaauaauaaa cacuggcuag uacauaaaga augguuucau     1260

gauauaccgu uaccuuggca ugcuggugcu gauacaggga cuccacauug gaauaauaaa     1320

gaagcauuag uagaauuuaa agaugcgcac gcgaaaagac aaacgguagu aguauuaggg     1380

ucacaggaag gagcaguaca uacugcacua gcaggagcau uagaagcaga aauggaugga     1440

gcuaaaggaa gacucagcuc agggcacuua aaaugcagau ugaaaaugga uaaauuaaga     1500

cuuaaaggug uuaguuauuc ucucuguacu gcagcauuua cuuucacuaa aauaccggca     1560

gaaacacuuc auggcacagu aacagucgaa guacaguacg caggaacaga ugggccaugu     1620

aaaguaccag cacaaauggc uguagauaug caaacguuaa caccuguagg acgauuaaua     1680

accgcaaacc ccguaauuac agaaucaaca gaaaacucaa aaaugaugcu ugaauuagac     1740

ccaccauucg gagauagcua uauaguaaua ggagugggag aaaagaaaau aacacaucau     1800

uggcauagaa gcgguucuac aauagguaaa gcauucgagg caacgguuag aggggcaaaa     1860

agaauggcgg uauuaggaga cacagcuugg gauuucggaa gcguaggugg agcauuaaau     1920

ucacuuggaa aagguauaca ucaaauauuu ggagcagcau ucaaaaguuu auucggaggu     1980

augucauggu uuagucaaau uuugauagga acauuacuga ugugguuagg guuaaauacu     2040

aaaaacggaa gcauuagcuu aaugugucuu gcucuaggag gcguauuaau auucuuaagc     2100

acagcaguua gugcagacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4740
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4740
gggagaaagc uuaccauggc uagaaaagaa aagaaacgaa gaggcgcaga uacaucagua       60

ggaauaguug gucuauuacu aacaacugca auggcagcag aaguaaccag aaggggaagu      120

gccuauuaua uguaucuuga uaggaaugau gcuggugagg caauaucauu uccuacaacu      180

cuuggaauga auaaaugcua cauacaaaua auggauuuag gucauaugug cgaugccaca      240

augucauacg aaugcccaau gcuagaugaa ggaguagaac cugaugaugu agacuguugg      300

ugcaacacaa ccaguacaug gguaguauau ggaacuuguc aucacaagaa aggagaagca      360

agaagaagua gacgagcagu aacgcuacca agccacucaa cacggaaauu acaaacaaga      420

agccaaacau gguuagaaag cagagaguau acgaaacacc ucaucagagu agaaaacugg      480

auauuuagaa acccgggauu ugcauuagca gcagcggcaa uagcauggcu auuaggaagu      540

uccacaaguc aaaaaguuau cuacuuagua augauacuuu ugauagcacc agcauacuca      600

auuagaugua ucgggguuuc uaauagagau uuuguagaag gaaugagugg agguacaugg      660

guagauauag uauuagaaca cggaggaugu gucacaguaa uggcacaaga uaaaccaaca      720

guugacauag aauuagugac aacaacagua agcaauaugg cagaaguacg auccuacugu      780

uacgaagcaa guauaagcga uauggcuagu gacucacgau guccuacuca aggugaggcu      840

uaccuagaua aacaaagcga uacgcaguau guaugcaaaa gaacauuagu agacagggga      900

ugggguaacg gaugcgggcu auuuggaaaa ggcucucuug uaacaugugc aaaauucgcu      960

uguaguaaga aaaugacggg uaaaucaaua caaccagaaa aucuagaaua uagaauaaug     1020

uuaaguguac auggaucuca acauaguggg augaucguaa augauacagg ucacgaaaca     1080

gacgaaaaca gagcuaaagu agaaauaaca ccaaauagcc cgagagcaga agcuaccuug     1140

ggaggauuug gaagccuagg ucuugauugu gaacccagaa caggauuaga uuucucagau     1200

uuauauuacc uaaccaugaa uaauaaacau uggcugguac auaaagaaug guuucaugau     1260

auaccuuuac cauggcacgc gggggcagac accgguacuc cacacuggaa uaauaaagag     1320

gcauuaguag aauuuaaaga cgcacaugca aaaaggcaaa caguaguggu acuagguagc     1380

caagaaggag caguacauac cgcauuagca ggagcacuag aggcugaaau ggauggagca     1440

aaaggaagac uaucaucagg acaucuuaaa ugucgauuaa aaauggacaa auuaagauua     1500

aaagggguaa gcuauaguuu augcacggca gcuuuuacau uuaccaaaau accagcagaa     1560

accuuacaug guaccguaac cguugaaguu caauaugcug gaacagacgg accauguaaa     1620

guacccgcac aaauggcagu agauaugcaa acacuaacac caguugggcg acugauaacu     1680

gcaaauccag uaauaacaga gucaacagaa aacucaaaga ugauguuaga auuagaucca     1740

ccauuuggug auucauauau aguaauagga guuggagaaa agaaaaucac acaucauugg     1800

cauagaagcg gaaguacuau aggaaaggcu uuugaagcaa caguaagagg agcaaaacga     1860

auggcagucu uaggagauac ugcuugggau uucggaagug uaggaggagc uuuaaacagc     1920

cucgguaaag ggauacauca aauauuugga gcagcauuua aaaguuuauu uggaggaaug     1980

aguugguuuu cacaaauauu aauuggcaca uuacuuaugu ggcuagguuu aaauacgaaa     2040

aacggaagca uauccuugau gugcuuagca cuuggagggg uacuaauauu cuugaguaca     2100

gcaguaagug cagauugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4741
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4741
gggagaaagc uuaccaugag aaaagagaaa aaacgaagag gcgcagauac uaguguaggc       60

aucguaggac uauuauuaac aacugcuaug gcagcagaag uuacaagaag aggcagcgca      120

uauuacaugu auuuagaccg aaaugacgca ggagaagcua uuucauuccc aacaacauua      180

ggaaugaaca aauguuauau acaaauuaug gacuuaggcc acauguguga ugcaacaaug      240

ucauaugaau guccaauguu agaugaagga guagaaccag augauguaga cuguuggugc      300

aauacaacaa guacaugggu aguauacggu acaugucauc auaaaaaagg ugaagcaaga      360

agaucuagga gagcaguaac cuuaccauca cauucaacaa gaaaauuaca gacaagaagu      420

caaacguggu uagaaaguag agaauauaca aaacaucuaa uuaggguaga aaauuggaua      480

uucagaaauc cgggauuugc auuagcagcc gcagcaauag cauggcuauu agggucuuca      540

acaagucaga aaguaauuua ccuaguaaug auauuacuaa uagcaccggc cuacaguaua      600

cgguguauag gaguaaguaa cagggauuuc guugaaggaa ugaguggagg uacaugggua      660

gacauaguau uagaacaugg uggaugugua acuguuaugg cacaagauaa accuacagua      720

gacauugaac uaguuacaac aacaguuagu aacauggcag aaguaaggag uuauuguuau      780

gaagcuucaa uaagugauau ggcaucagau ucacguuguc cuacacaagg agaagcuuau      840

uuagauaaac aaagugauac acaauacgua uguaaaagaa cauuaguaga ucgaggaugg      900

ggaaaugguu guggucuauu cggcaaaggu ucuuuaguaa ccugugcaaa auuugcuugc      960

agcaaaaaaa ugacuggaaa aaguauccaa ccagagaacu uagaauauag aauaauguua     1020

aguguacaug gaagccaaca cucaggaaug auaguaaaug auacaggaca ugaaaccgau     1080

gagaauagag cuaaaguaga aauaacacca aacucaccuc gagcugaagc aacauuagga     1140

ggauucggau cguugggauu agacugugaa ccgagaacug gacuagauuu uucugauuua     1200

uauuauuuaa caaugaauaa uaaacacugg uuaguucaua aagaaugguu ucaugauaua     1260

ccauuaccuu ggcaugcagg ugcugauacu ggaacaccac acuggaauaa uaaagaagca     1320

uuaguagaau uuaaggaugc acacgcgaaa agacaaacag uaguaguuuu aggaagccaa     1380

gagggagcag uacacacagc ucuagcagga gcguuagaag cagaaaugga uggugcaaaa     1440

ggacgauuau caucaggaca ucuuaaaugu cgacuaaaaa uggacaaacu gagacuaaaa     1500

ggaguaucau auaguuuaug uacagcagcg uuuacauuua cuaaaauacc ugcggaaaca     1560

cuucauggaa cuguuacggu agaaguacaa uaugcaggaa cugauggacc augcaaagua     1620

ccggcccaaa uggcaguaga caugcaaacu uuaacaccag ucggaagauu aauaacugca     1680

aaccccguaa uaacagaaag cacugaaaac aguaaaauga uguuagaacu ugauccccca     1740

uucggagaua gcuauauagu uauaggcgua ggagaaaaga aaauaacaca ucauuggcac     1800

agaucaggau caacaauagg aaaagcuuuu gaagcuacag ugagaggagc aaaaagaaug     1860

gcaguauugg gagauacagc uugggauuuc gguucuguug gcggagcgcu aaauagucua     1920

ggaaaaggaa uacaucaaau auucggugcu gcauuuaaau cauuguucgg aggcauguca     1980

ugguuuagcc aaauuuuaau aggaacacua uuaauguggu uaggauuaaa caccaaaaau     2040

ggcucuauuu cacuaaugug cuuagcauua ggagguguau uaauauucuu aaguacagcg     2100

guuagugcgg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4742
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4742
gggagaaagc uuaccaugaa agagaaaaaa agaagaggag cagauacguc aguaggaauu       60

guaggauuau uauugacaac agcaauggca gcugaaguga cucguagagg aucagcauac      120

uauauguauu uggaucguaa ugacgcaggu gaagcaauuu cuuuuccuac aacauuaggu      180

augaauaagu guuauauaca aauaauggac uuaggacaca ugugugaugc aacaauguca      240

uaugaaugcc cuauguuaga cgaaggggua gaaccagacg auguugauug uugguguaau      300

acuacaucaa cauggguagu auacggaaca ugucaccaua aaaaaggaga agcacgcaga      360

aguagacgag caguaacacu accuucacau ucaacaagaa aacuacagac gagaucacaa      420

acuuggcuag aaaguagaga auacacaaaa caucuaauaa gaguagaaaa uuggaucuuu      480

agaaauccag guuuugcguu agcagcagca gcaauagcuu gguuguuagg gucgucaacu      540

agccaaaaag uaauauaucu aguaaugaua uuauuaaucg caccggcuua uaguauaaga      600

ugcauaggag uuaguaaucg agauuuugua gagggaaugu caggaggaac uuggguagau      660

aucguucuag aacacggagg auguguaaca guaauggcac aggauaaacc aacaguugau      720

auugaauuag uaacaacaac aguaagcaac auggcagaag uaaggaguua uugcuacgaa      780

gcuaguauau cagauauggc aagugauuca agauguccua cacaagguga agcauauuua      840

gauaaacaaa gcgacacaca auaugucugu aaaagaacau ugguugauag agguugggga      900

aaugguugcg gauuauucgg caaagguucu cuaguaacuu gcgcaaaauu ugcauguuca      960

aaaaaaauga cagguaaaag cauacaacca gaaaacuuag aauauaggau uauguuaucg     1020

guucauggcu cgcaacauag cgguaugauu guaaaugaua caggacacga aacagacgaa     1080

aacagagcaa aaguagaaau aacuccaaau agcccaagag cagaagccac acuaggugga     1140

uuugguaguu uaggacuaga uugugaacca agaacaggau uagauuuuag ugaccuauau     1200

uauuuaacaa ugaauaauaa acacugguua guucauaaag aaugguuuca cgauauucca     1260

uuaccuuggc augcaggugc ggacacagga acgccacauu ggaacaauaa agaagcuuua     1320

guagaauuca aagaugcuca ugcaaaaaga caaacaguag uaguacuagg aagucaagaa     1380

ggagcgguac auacagcacu agcaggugca uuagaagcag aaauggaugg agcgaaaggc     1440

agacuaucau cuggacaucu aaaauguaga uuaaaaaugg acaaacuaag gcuaaaagga     1500

guaaguuacu cacuauguac agcagcauuc acauuuacaa aaauuccagc cgaaacguua     1560

cacggaacag uaacggucga agugcaauau gcaggaacug auggacccug uaaaguaccu     1620

gcucaaaugg caguagauau gcaaacacuu acaccaguag gaagauuaau aacagcuaau     1680

ccaguaauaa cagaaucuac agagaacuca aaaaugaugc ucgaauuaga cccaccauuc     1740

ggugauaguu auauaguaau aggaguagga gaaaagaaaa uaacacauca cuggcacaga     1800

ucagguucaa caauuggaaa ggcauuugaa gcuacuguua gaggagcaaa acgaauggca     1860

guauugggug acacggcaug ggauuuugga ucaguaggag gagcuuugaa uucucuaggc     1920

aaaggaauac accaaauauu cggagcagcc uucaaaucuc uauuuggagg uaugagcugg     1980

uucucacaaa uauuaauagg uacuuuauua augugguuag gauuaaacac uaaaaaugga     2040

ucaauauccc uaauguguuu agcguuagga gguguauuaa uauuccuaag uacagcuguc     2100

agcgccgauu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4743
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4743
gggagaaagc uuaccaugga aaaaaaaaga cgaggggcgg auacaucagu aggaauaguu       60

gguuuauuau uaacaaccgc aauggcagca gaaguaacua gaagaggauc agcauauuau      120

auguauuuag auagaaauga ugcaggagag gcaauaucau uuccuacaac acuuggaaug      180

aauaaauguu acauacagau uauggauuug ggacacaugu gugacgcaac aaugaguuac      240

gaaugcccaa ugcuagauga agggguagaa ccagacgacg uggauuguug guguaacaca      300

accucaacgu gggucguaua cggaacaugc caccauaaaa aaggagaagc ccgacggagu      360

cgaagagcag ugacauuacc aagucauuca acgagaaaau uacaaacuag aagccaaaca      420

ugguuagaaa gccgagaaua cacaaaacau cuuauucgag uagaaaauug gauauuuaga      480

aaucccgggu uugcacuagc agcagcagca auagcauggu uacuaggauc aucaacaucc      540

caaaaaguga ucuacuuagu aaugauauug cuuauagcgc cagcauauag uauaaggugu      600

aucggaguaa guaauagaga uuucguagaa gguaugagug gaggaacaug gguugacaua      660

guuuuggagc auggcgggug cguaacagua auggcacaag acaaacccac gguagacaua      720

gaauuaguga cuacuacugu aucaaauaug gcagaaguua gaagcuacug uuacgaagca      780

ucaauaucug acauggcaag ugauaguaga uguccaaccc aaggagaagc auaucuagau      840

aaacaaucag auacacaaua cguauguaaa agaacucuug uagaucgagg uuggggaaau      900

gguugcggcu uauuuggaaa aggaaguuua guaacaugug caaaauuugc auguucuaaa      960

aaaaugaccg guaaaucgau acagccggaa aaccuagaau accgaauuau guuaagugua     1020

caugguucgc agcauucagg aaugauagug aacgacacag gucaugaaac agaugaaaau     1080

cgugcuaagg uagaaauuac accaaacucg ccaagagcag aagcaacacu aggaggauuu     1140

gguucacugg gauuggauug ugaaccuaga acaggccuug auuucucaga uuuauauuau     1200

uuaacaauga auaauaaaca uuggcuugua cauaaagaau gguuucauga uauaccauua     1260

ccauggcacg cuggagcaga cacggguaca ccacauugga acaacaaaga agcacuagua     1320

gaauuuaagg acgcccaugc aaaaagacaa acugugguag uacuagguag ucaagaagga     1380

gcuguacaua cugcucuggc aggggcauua gaagcugaaa uggauggugc aaaaggaaga     1440

uuaucaagug gacacuuaaa augcagacua aaaauggaca aauuaagauu aaaaggaguu     1500

ucauauaguu uauguacagc agcauuuacu uuuacaaaaa uuccagcaga aacauuacau     1560

ggcacaguaa cuguagaagu ccaauaugca ggaacagacg gaccuuguaa aguuccagca     1620

caaauggcag uagacaugca aacguuaaca ccaguuggaa gacuuauaac ugcaaaucca     1680

guaauaacag aaaguacaga gaauaguaaa augaugcuag aauuagaucc accuuucgga     1740

gauucauaua uuguaauagg uguaggagaa aaaaaaauaa cacaccacug gcaucgaagu     1800

gguaguacua uaggaaaagc auuugaagcu acagucaggg gagcaaagag aauggccgua     1860

cuaggagaua cagcauggga cuucggaagc guuggaggug cccuaaauuc auuagggaaa     1920

gguauacacc aaauauucgg cgccgcauuu aaaagccuau uuggaggaau gaguugguuu     1980

ucacaaauac uaauaggcac auuauuaaug ugguuaggcu uaaacacaaa aaauggauca     2040

auaagucuaa uguguuuagc auuaggagga guauuaauuu uucuaucaac agcuguaucu     2100

gcugacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4744
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4744
gggagaaagc uuaccaugaa aaaaagaaga ggagcagaca caaguguagg aauaguagga       60

cuauuauuaa caacugcaau ggcagcagaa guaacaagaa gaggaucagc guauuauaug      120

uaucucgaua gaaaugaugc aggagaggca auauccuucc caacaacucu uggaaugaac      180

aaaugcuaua uacaaauuau ggauuuagga cauaugugcg augcaaccau guccuacgaa      240

uguccaaugu uagaugaagg uguagaaccu gaugauguag auuguuggug uaauacaaca      300

aguacuuggg ugguauaugg aacaugccau cauaaaaaag gagaggcaag aagaagcagg      360

agagcgguaa cacuaccauc ccauagcaca cgaaaacuac aaacuagaag ucaaacaugg      420

cuagaaagca gagaauacac uaaacaucuu auaagaguag aaaauuggau auuuagaaac      480

ccaggguuug cgcuagcagc cgcagccauu gcuugguuau uaggcucauc aacaagccaa      540

aaaguaauau accuaguaau gauacuacua auagcgccug cauauucaau uagaugcauu      600

ggagucucca auagagauuu uguagaagga augaguggag ggacaugggu cgacauagua      660

cuagaacacg gagguugugu uacagugaug gcgcaagaua aaccaaccgu cgauauagaa      720

uuaguaacua caacaguauc aaauauggca gaaguaagau ccuauuguua ugaagcaucg      780

auaagcgaua uggccucaga uucuagaugu cccacacaag gagaagcuua cuuagauaaa      840

caaucugaua cacaauaugu cuguaaaaga acacucguag auagaggaug gggaaaugga      900

uguggccuau ucggaaaagg uaguuuagua acaugugcaa aauuugcaug uagcaaaaaa      960

augacaggaa aaagcauuca accagaaaau cuggaauaua gaauaauguu aucuguacau     1020

ggaagucaac auucagggau gauaguuaau gauacaggac acgaaacaga ugaaaaucga     1080

gcaaaaguug aaauaacacc uaauagucca agagcagaag caacauuagg aggauuugga     1140

agucuaggau uagacuguga accuagaacu ggauuggauu uuagugacuu auauuaucuu     1200

acuaugaaua auaaacacug gcuuguucau aaagaauggu uccacgauau accacuacca     1260

uggcaugcag gcgcugacac agguacaccc cauuggaaua acaaagaagc acuaguagaa     1320

uucaaagaug cucaugcaaa aagacaaaca gucguaguuc uaggaaguca agaaggagca     1380

guacacacag cuuuagcugg agcauuagaa gcagaaaugg auggugcaaa aggacgacua     1440

ucaucaggac acuuaaaaug cagacuaaaa auggauaaau uaagacuaaa aggaguauca     1500

uauaguuugu guacagccgc auuuaccuuu acaaaaauuc cagcugaaac auuacacgga     1560

acuguaacag uggaaguaca auaugcaggu acagacggac cguguaaggu accagcucag     1620

auggcaguag auaugcaaac auuaacgccc guugguagau uaauaacugc aaauccagug     1680

auaacagaau caacagagaa uagcaaaaug augcuagaau uagauccccc auuuggagau     1740

agcuauauag uaauuggagu aggagaaaaa aaaauaacac aucauuggca ucgaucagga     1800

uccacuauag guaaagcuuu ugaagcaaca guaagagggg cuaaaagaau ggcgguauua     1860

ggagauacag ccugggauuu uggaucaguc ggaggugcau uaaacaguuu agguaaagga     1920

auucaucaaa uauuuggagc agcauucaaa agcuuauuug gagguauguc augguuuucg     1980

caaauacuga uuggaacauu gcuaaugugg cuaggacuaa auacuaaaaa uggaaguauc     2040

ucauuaaugu gucuagcacu aggaggugua uuaauauuuc ucagcacagc agucucagca     2100

gauugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4745
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4745
gggagaaagc uuaccaugaa aagaagaggu gcagauacau caguaggaau aguuggguua       60

uuacuaacaa cagcaauggc agcagaagua acgagaaggg guagugcgua cuauauguac      120

cuagaccgaa augaugcagg ugaagcuaua agcuuuccaa ccacacuagg caugaauaaa      180

uguuauauac aaauaaugga ucuaggacau augugugaug cuacaaugag uuacgaaugu      240

ccaaugcuag acgaaggagu agagccagac gauguagacu guugguguaa uacgacauca      300

acauggguag uauaugguac augucaucac aaaaaaggcg aagcaagacg aucaaggaga      360

gccgucacuc uaccaaguca cagcacaaga aaacuacaaa cuagaaguca aacaugguua      420

gaaucaagag aauauacaaa acaucuaaua aggguggaaa acuggauauu cagaaaucca      480

gguuuugcac uagcagcagc agcgauagcu ugguuauuag guaguucuac uucacaaaaa      540

guuauauacu ugguaaugau uuuauuaaua gcacccgcau acaguauaag gugcauugga      600

guaaguaaua gagauuuugu agagggaaug aguggaggaa cauggguaga uauaguauua      660

gaacauggag gcuguguaac ugugauggca caagacaaac cuacaguaga cauugaauua      720

guaacgacaa caguuucaaa uauggcagaa guaagaucuu auuguuauga agcaaguaua      780

agcgauaugg caagugacag cagaugucca acacaagggg aagcuuaucu ugacaagcaa      840

ucagacacgc aauacguuug uaaacggaca uuaguagaua ggggcugggg gaacggaugu      900

ggauuauuug gaaaaggguc cuuaguaaca ugcgcaaaau ucgccuguag uaaaaaaaug      960

acaggaaaau cuauacaacc agaaaaucuc gaauauagaa uaauguuauc aguacaugga     1020

agucaacauu cagggaugau aguaaaugau acuggccaug aaacagacga aaauagagca     1080

aaaguagaaa uaacaccuaa uagucccaga gcagaagcaa cauuaggagg auucggauca     1140

uuaggauuag acugugaacc aagaacaggc cuagauuucu cugacuuaua uuaccuaaca     1200

augaauaaua aacacuggcu aguucacaaa gagugguuuc augacauacc gcuaccuugg     1260

caugcaggug cagauacagg aacgccacau uggaauaaua aagaagcacu aguagaguuu     1320

aaagaugcuc augcaaaaag acaaacggua guaguauuag gaagccaaga aggagcagua     1380

cacacagcuc uagcaggagc ucuugaagca gaaauggaug gugcuaaagg ucgauuauca     1440

uccggacacu uaaaauguag auuaaaaaug gacaaacuaa gauuaaaagg aguaagcuac     1500

agccuaugua cagcagcauu uacauuuaca aaaauaccag cagaaacuuu acauggaaca     1560

guaacaguug aaguacaaua ugcaggaaca gaugggccau guaaaguucc ugcgcaaaug     1620

gcaguagaca ugcaaacauu aacaccagua ggaagauuga uaacagcaaa uccaguaaua     1680

acugaaucaa cugaaaacuc aaaaaugaug cuagaacuag acccaccauu cggagauagc     1740

uauauaguaa uuggaguugg cgaaaaaaaa auaacacauc auuggcacag aaguggaagu     1800

acgauuggaa aagcauuuga ggcaacagua agaggugcua aaagaauggc aguacuugga     1860

gacacagcuu gggauuuugg uagcguagga ggagcauuaa auucacuugg aaaaggaauu     1920

caucaaauau ucggagcagc cuucaaauca cuauuuggag gaaugaguug guuuucacag     1980

auauuaauug ggacacuucu aauguggcua ggauugaaua caaaaaaugg gaguauaucu     2040

uuaauguguc uagcauuagg ggguguauua auauucuugu caacagcugu gucugccgau     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4746
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4746
gggagaaagc uuaccaugag aagaggcgca gauacaucag ucggaauugu uggucuuuug       60

uuaacuacag caauggcagc agaaguaaca agacguggaa gugcguacua cauguaccua      120

gacagaaaug augcaggaga agcaaucuca uuuccuacua cucuggguau gaacaaaugc      180

uauauacaaa uaauggaucu aggccauaug ugugaugcga caaugucaua ugagugucca      240

augcuagacg aagguguuga accugacgau guagauugcu gguguaacac aacaucaaca      300

uggguaguau auggaacaug ccaccauaag aaaggagaag cuagaagauc aagacgagca      360

guaacauuac ccagucauag uacaagaaaa uuacagacaa gaagccaaac auggcuagaa      420

ucgagagaau auacuaaaca ucuaauaaga guagaaaauu ggauauuccg aaacccagga      480

uuugccuugg cagcagcugc caucgcaugg cuuuuaggaa guucgacaag ccaaaaagua      540

auauaucuag uuaugauacu ucucauagca ccagcuuauu caauaagaug uaucgguguu      600

aguaacagag auuuuguaga agguaugucc ggagguacuu ggguugauau aguauuagaa      660

caugguggau guguuacugu aauggcacaa gauaaaccaa caguagauau agaauuagua      720

acaacuacag uaagcaacau ggcagaagua agaucuuauu guuaugaagc aucaauauca      780

gauauggcaa gugauaguag augcccaacc caaggagaag cauacuuaga uaaacaguca      840

gauacacaau auguauguaa acgaacuuua guugaucgag gguggggaaa cggauguggu      900

uuauuuggaa aaggaaguuu aguaacaugu gcuaaauuug caugcucaaa gaaaaugaca      960

ggaaaaucaa uacaaccaga aaauuuagaa uauaggauaa ugcuuagcgu acauggauca     1020

caacauuccg gaaugauugu aaaugacacg ggacaugaaa cugaugaaaa cagagcuaaa     1080

gucgaaauaa caccuaauag uccucgagca gaagcaacau uaggaggauu ugguagcuua     1140

ggacuugacu gugaaccuag aacaggacua gauuuuagug aucuauacua ccuuaccaug     1200

aauaauaaac auuggcuagu acacaaagaa ugguuucaug acauaccauu accauggcau     1260

gccggagcag acacagguac accucacugg aauaauaaag aagcccuagu agaauuuaaa     1320

gaugcacacg caaaacgaca aacaguggug gucuuaggaa gccaagaagg agcaguacau     1380

acagcauuag ccggugcucu agaagcagaa auggauggug caaaaggaag acuuagcuca     1440

ggacauuuaa aauguagguu aaaaauggau aaauuaaggu uaaaaggagu aucauauagu     1500

cuaugcacag cagcauuuac uuucacaaaa auacccgccg aaacgcuaca cggaacagua     1560

acaguagaag uacaauacgc aggaacugau gguccaugua aaguaccagc ucaaauggca     1620

guagacaugc aaacauuaac accaguaggu agguuaauaa ccgcgaaucc aguaauaacu     1680

gaaucaacag aaaauagcaa aaugauguua gaacuagacc cuccauucgg agauagcuau     1740

auaguaauag guguaggaga gaagaaaauc acacaucauu ggcauagaag uggaucaaca     1800

auaggaaaag cauuugaagc uacaguaaga ggagcaaaac guauggcagu auugggagau     1860

acugcauggg auuuuggaag uguaggggga gcucuaaauu cacuuggcaa aggaauacau     1920

caaauauuug gagcagcauu uaaauccuua uucggaggaa ugucuugguu uagccaaaua     1980

cuaauaggaa cuuuauuaau guggcuugga cuaaacacua aaaauggauc aauuucuuua     2040

augugucuag cucuaggagg aguacuuauc uuucuaagua cagcagugag ugcagacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4747
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4747
gggagaaagc uuaccaugag aggagcagac acaaguguag guauaguugg auuauuauua       60

acaacagcaa uggcagccga aguaacaaga agagguucag cuuauuauau guauuuagac      120

agaaaugaug caggagaagc aauaucauuc ccaaccacgc uuggaaugaa uaaaugcuau      180

auacaaauaa uggauuuagg acacaugugu gaugcaacaa ugucauauga augccccaug      240

uuagaugaag guguagaacc agaugacgua gacuguuggu guaauacaac gagcacaugg      300

gucguauaug gaacuugcca ucauaagaaa ggagaagcua gacgaagcag acgagcagua      360

acgcuaccaa gucacucaac cagaaaauug caaacaagga gucaaacaug gcuagaauca      420

agagaauaua cuaaacaucu uaucagagua gaaaauugga uuuuucgaaa uccaggauuu      480

gcacuugcug cagcugcuau agcauggcua uuagguucaa gcaccucaca aaaaguaaua      540

uaucuaguua ugauacuauu aauugccccu gcauacucaa uuagauguau aggaguauca      600

aauagagacu ucguagaggg aaugucagga ggaacuuggg uagauauagu acuagaacau      660

gguggaugug uaacaguaau ggcacaagau aaaccaacug uugacauaga auuaguaaca      720

acaaccguaa guaauauggc agaaguaaga uccuacugcu augaggcaag uauaagcgau      780

auggcaucag auagucgaug uccaacacaa ggggaagcau auuuagauaa acaaucagau      840

acgcaauacg ucuguaaaag aacauuagug gacagaggau gggguaaugg uuguggacua      900

uucggaaaag guagcuuagu uacaugugca aaauuugcau guaguaagaa aaugacagga      960

aaaucaauuc aacccgaaaa cuuagaauau agaauaaugc uaucaguaca uggaagucaa     1020

cauucaggua ugauuguaaa cgauaccggc caugaaaccg augaaaauag agcuaaagua     1080

gaaauaacgc cuaauagccc aagagcggaa gcaacacugg gaggauuugg gagcuuagga     1140

uuggauugug aaccaagaac aggacuagau uucucugacu uauauuaccu aaccaugaau     1200

aauaaacauu gguuaguaca uaaagaaugg uuucaugaua uaccacuacc guggcaugca     1260

ggggcagaua ccggaacccc acacuggaau aauaaagaag ccuuaguaga auuuaaagac     1320

gcgcaugcaa aaagacaaac uguaguagua uuaggaucac aagaaggggc aguucacacc     1380

gccuuagcag gagcacuaga agcagaaaug gauggagcua aaggucgacu aucaucagga     1440

cauuuaaaau guagacuuaa aauggauaaa uuaagauuaa aaggaguuag uuacucauua     1500

ugcacugcag cauucacuuu caccaaaaua ccagcagaaa cauuacaugg cacuguaaca     1560

guagagguac aauaugccgg aacagaugga cccugcaaag uaccagcgca gauggcuguc     1620

gauaugcaaa cauuaacucc aguuggucga cucauaacug cuaaucccgu aauaacagaa     1680

ucaacagaaa auucaaaaau gaugcuugaa uuagauccuc ccuuuggaga uucauauaua     1740

guaauaggag uaggggaaaa gaaaauaacc caccauuggc auagaagugg aaguacaauu     1800

gguaaagcau uugaagcaac aguuagagga gcaaaaagaa uggcgguauu aggagauaca     1860

gcgugggacu ucggcagcgu aggaggcgcc uuaaauucuu uaggaaaagg gauacaucaa     1920

auauuuggag cagcauucaa aucauuauuc ggaggaaugu caugguuuuc acaaaucuua     1980

auaggaacuc uauuaaugug guuaggucua aauacuaaaa acggcucaau aaguuuaaug     2040

uguuuggcac uaggcggagu acuaauuuuc cuaucgacag caguauccgc agacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4748
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4748
gggagaaagc uuaccauggg agcggauaca ucaguuggua uaguaggacu guuauuaaca       60

acagcuaugg cugcagaagu aaccagaaga ggaucagcau auuauaugua uuuagauaga      120

aaugaugcug gagaagcuau aucauuuccc accaccuuag gaaugaauaa auguuacauu      180

caaauaaugg auuugggaca caugugcgau gcaacaauga gcuaugagug ucccauguua      240

gacgaaggag uugaaccaga ugacguagau uguuggugua auacgacaag uacuugggua      300

guauacggca caugucauca caagaaagga gaagcacgcc gcucaagacg agcuguaacc      360

cuucccaguc auaguacaag gaaauugcaa acucguagcc aaacaugguu agaauccaga      420

gaauauacca aacaucucau aagaguagaa aacuggauau uuagaaaucc aggauucgca      480

uuagcugcag cagcaauagc auggcuccua ggaucaagca caucccaaaa aguuauauac      540

cuaguuauga uauuauuaau agcuccagca uauucaauua gauguauagg aguuucaaac      600

cgugacuuug uugaaggcau gucaggagga acauggguag auauaguacu agaacacgga      660

ggauguguaa cuguaauggc acaagacaaa cccacaguug auauagagcu aguuacuaca      720

acaguaagua auauggcaga aguaagaagu uauugcuacg aagcaucaau aagugauaug      780

gcaagugaca guagaugccc aacccaagga gaagcguacu uagacaaaca aagugauacc      840

caauauguuu guaaaagaac acuuguugau cgaggauggg gaaauggaug cggacucuuu      900

gguaaaggau cauuaguaac augugcaaaa uuugccugua gcaagaaaau gacaggaaaa      960

ucaauacaac cagaaaaccu agaauauaga auaauguuau cuguacaugg uucacaacau     1020

ucagguauga uugucaacga cacaggacau gaaacugaug aaaauagagc aaaagucgaa     1080

auaacuccaa auucaccacg agcagaagcc acuuuagggg guuuuggaag ucuaggauua     1140

gacugugaac caaggacagg auuagauuuu ucagaucuau auuauuuaac aaugaacaac     1200

aaacauuggc uaguacacaa agaaugguuu caugacauac cauuacccug gcacgcaggc     1260

gcugacacug gaacuccgca cuggaauaau aaagaagccc uaguugaguu caaagacgca     1320

caugcaaaaa gacaaacagu aguuguauua ggaucacaag aaggagcugu acauacagca     1380

uuggccggag cauuagaagc ugaaauggau ggagcaaaag guagacuaag cagcggccau     1440

cucaaaugca gauuaaaaau ggacaaacua agacuaaaag gcguuucaua cucacuaugu     1500

acagcagcuu uuacauuuac uaaaauaccu gcagaaacau uacaugguac aguaacaguu     1560

gaaguacaau augcaggaac agauggacca ugcaaaguac cagcacaaau ggcagucgau     1620

augcagacau uaacgccagu aggacgacua auaacagcaa auccuguaau aacagaaagc     1680

acagaaaauu caaaaaugau gcuagaacuc gacccucccu ucggggauuc auauauagua     1740

auaggaguag gcgaaaagaa aauaacacac cauuggcauc guucaggaag uacuauagga     1800

aaagcauucg aagccacagu aagaggugca aaaaggaugg caguguuagg agauacagca     1860

ugggauuuug gaucagucgg aggagcacua aauagcuuag gaaaaggaau acaucagaua     1920

uuuggagcag cauuuaaaag uuuauucggu ggaaugucuu gguucucuca aauuuuaaua     1980

ggaaccuuac uuaugugguu ggggcuaaau accaaaaaug gaucaauauc auuaaugugc     2040

uuagcucuag gagguguacu aauauuucua ucgacagcag uguccgcaga uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4749
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4749
gggagaaagc uuaccauggc ugauacauca guaggcauag uaggucuacu uuuaacaaca       60

gcaauggcag cugaaguaac aaggcgaggu agcgcuuauu acauguacuu agauagaaau      120

gaugcagggg aagcuauauc uuuuccgaca acauuaggaa ugaauaaaug cuauauucaa      180

auaauggacc ugggacauau gugugaugcg acaaugaguu augaaugccc aauguuagau      240

gaaggaguug aaccggauga cguagacugc ugguguaaua caacaaguac augggucgua      300

uaugguacau gucaucauaa aaaaggagaa gcaagaagau caagaagagc aguuacauua      360

ccuucacacu cuacaagaaa auugcaaaca cgaagccaaa cguggcuuga aucaagagag      420

uacacaaaac auuuaauccg aguagaaaau uggauuuuca gaaauccagg auucgcacua      480

gccgcagcag caauagcaug guuacucgga ucaaguacau cacaaaaagu aauuuacuua      540

guaaugauau uacuaauagc cccagcauau aguauaagau guauaggagu aaguaauaga      600

gauuuugugg aaggaaugag uggagguacg uggguagaua uuguauuaga acauggugga      660

uguguaacag uaauggcaca ggauaaacca acaguagaca uugaauuagu aacaacaacc      720

guaucaaaca uggcagaagu aagaucauac uguuaugaag caaguauauc ugauauggcg      780

agcgacucaa gauguccuac acaaggugaa gcguaucuag auaaacaauc cgauacucaa      840

uauguaugua aaagaacccu aguagacaga ggguggggca augguugugg uuuauuuggc      900

aaaggaucau ugguaacaug ugcaaaauuc gcaugcucaa aaaaaaugac uggaaaauca      960

auucagccgg aaaaccuaga auauagaaua auguuaucug uucacggaag ucaacacuca     1020

ggaaugauug uaaacgauac uggucacgaa acugaugaaa auagagcuaa aguagaaaua     1080

acaccaaaua guccaagagc agaagccacc cuugguggau ucggaagucu aggauuagau     1140

ugcgaaccaa ggaccggauu agacuucuca gauuuauauu aucuaacuau gaauaauaaa     1200

cauuggcuag uacacaaaga augguuucac gauauaccuu uaccauggca ugcaggagca     1260

gauaccggua caccacacug gaacaauaaa gaagcuuuag uagaauuuaa agaugcucac     1320

gcaaaacgac aaacgguugu aguacuagga ucacaagaag gagcagucca cacagcauua     1380

gcgggagccu uagaagcaga aauggaugga gcaaaaggaa gacuuaguuc aggacauuua     1440

aaaugccgau uaaaaaugga uaagcuuaga cuaaaaggag uaaguuacag ccugugcaca     1500

gcugcauuua cauuuacaaa aaucccagcg gaaacauuac auggaaccgu cacaguagaa     1560

guacaauacg ccggaacaga uggcccaugc aaaguaccag cucaaauggc uguagauaug     1620

caaacacuaa caccaguagg aagacuaaua acagccaacc caguaauaac cgagucuaca     1680

gaaaacagua aaaugauguu agaacucgau ccuccauuug gagauagcua uauaguaauu     1740

gguguaggag aaaaaaaaau aacacaucau uggcaucgau cagguucaac aauagguaaa     1800

gcauuugaag cuacaguacg aggagccaaa agaauggcag uauuggguga uacagcaugg     1860

gauuuuggau cugucggagg agcauuaaac ucauuaggaa aagguauaca ucaaauauuu     1920

ggugcugcau ucaaaagcuu auuuggagga augucauggu uuucccaaau ucuaauagga     1980

acauuauuaa ugugguuagg auuaaacacg aaaaaugggu caauaagucu aaugugccuu     2040

gcauuaggug gaguacuaau auuucuaagc acugcaguau cagcugauug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4750
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4750
gggagaaagc uuaccaugga cacaagugua ggaauaguag gauuguuauu aacaacugca       60

auggcagcag aagucacucg ucgugguagc gcauacuaua uguauuuaga uagaaaugau      120

gcaggagaag caauaucuuu uccgacaaca uuaggaauga acaaauguua uauacaaaua      180

auggauuuag gucauaugug ugacgcuacg augucauaug aauguccaau guuggaugaa      240

ggaguagaac cagaugacgu cgauuguugg ugcaauacga cauccacaug gguaguuuau      300

gggacauguc aucauaagaa aggagaagcg aggagaagca ggagagcagu aaccuuacca      360

agucacagca caagaaaacu acaaacaaga agucaaacau gguuagaauc aagagaauau      420

accaaacacu uaauaagggu ggaaaauugg auauuucgaa auccaggauu ugcauuagca      480

gcagcugcaa ucgcgugguu auuaggauca ucaacaucac agaaaguaau auaccuaguu      540

augauauuau uaauagcacc agcauauagc auaagaugca uaggagugag uaauagagau      600

uuuguagaag gaaugagugg aggcaccugg guagauauag uacuagaaca uggcggaugu      660

gucaccguua uggcucaaga uaaaccaaca guagauauag aauuaguaac uacaacagua      720

ucaaauaugg cagaaguaag aaguuacugc uaugaggcuu caauaaguga cauggcuuca      780

gauucaagau guccgacaca aggagaagca uauuuagaua aacagucuga uacucaauau      840

guaugcaaaa gaacccuagu agacagagga uggggaaaug guugugguuu auucggaaaa      900

ggaagucugg uuacaugugc aaaauucgca ugcucuaaga aaaugacagg aaaaucaauc      960

caaccagaaa accuagaaua cagaauaaug uuaucaguac acggcucaca acauagugga     1020

augauaguga augauacugg acacgaaacu gaugaaaauc gagcaaaagu agaaauaaca     1080

cccaacaguc cgagagcaga agcuacauua ggaggauuug guucauuagg auuggauugc     1140

gaaccaagaa cagguuuaga cuuuagugau uuauauuauc uuaccaugaa uaacaaacau     1200

uggcuuguac auaaagaaug guuucaugac auuccucuac cauggcaugc cggagcagau     1260

acggguacuc cacacuggaa uaauaaagaa gcauugguag aauuuaaaga ugcacaugca     1320

aaaagacaaa cuguaguagu auuaggaagu caagaaggag caguucauac ugcguuagcu     1380

ggagcauuag aagccgaaau ggauggagca aagggaagau uaucaagugg acaccuuaaa     1440

ugccgacuga aaauggauaa acuaagacuu aaagguguuu cuuauucuuu auguacugcg     1500

gcuuucacau uuacgaaaau acccgcugaa acacuucaug guacuguaac aguagaagua     1560

caauaugcag gaacagacgg accauguaaa guaccagcac aaauggcagu cgauaugcaa     1620

acacuuacac caguagguag acuuaucaca gcgaauccag uuauaacuga aaguacagaa     1680

aauaguaaaa ugaugcugga auuagauccu ccuuucggag acaguuacau aguuauuggu     1740

guaggggaaa agaaaauaac acaucacugg cauagaagug gaucaacuau aggaaaagca     1800

uuugaagcaa caguccgugg agcaaaacga auggcaguac uaggcgauac ugcaugggau     1860

uuugguagcg uugguggagc acuaaauuca cuuggaaaag gaauacacca aauauuugga     1920

gcagcauuua aaucacuuuu ugguggcaug ucaugguuuu cacaaauuuu aauuggaaca     1980

uuauuaaugu ggcuaggauu aaauacuaaa aacgguagua uaucguuaau gugucuagca     2040

cuagguggag ucuuaauauu ucuuaguaca gcaguuagug cagauugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  4751
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4751
gggagaaagc uuaccaugac aagugucgga auaguaggau uauuguuaac aacggcaaug       60

gccgcagaag ugacgagaag ggggucugcu uauuauaugu aucuugauag aaaugaugca      120

ggagaagcua uaaguuuucc aacaacucua ggaaugaaua aauguuacau ucagauaaug      180

gacuuagggc auaugugcga ugcaacaaug ucauacgagu gcccaaugcu agaugaagga      240

guagaaccag augacguuga cugcuggugu aauacaacuu caacaugggu agucuacggu      300

acaugucauc acaaaaaagg agaagcaaga agaucuagaa gggcagugac auuaccuagu      360

cauagcacga ggaaacucca aacccgauca caaacauggc uagaaagcag agaauauaca      420

aagcaccuaa uaagaguaga aaauuggauu uuuagaaauc caggauucgc auuagcagcc      480

gcugcgauug caugguuacu uggaucaagu acgucgcaaa aaguuauaua cuuaguaaug      540

auauugcuaa ucgcaccagc auauucgauc agaugcauag gaguaucaaa cagagacuuc      600

guagaaggaa ugaguggagg aacaugggua gauauaguac uagaacaugg agguugugug      660

acaguaaugg cacaagauaa accaacugug gacauagaac uagugacaac aacaguauca      720

aacauggcag aaguacgauc uuacuguuau gaagcgucaa uaagcgauau ggcaagcgau      780

aguagaugcc caacacaagg agaagcauac uuagauaaac aaucagauac ucaauaugua      840

uguaaacgaa cacuaguaga uagaggaugg ggaaauggau guggauuauu uggcaaagga      900

agucugguaa cuugugcaaa auuugcuugc aguaaaaaaa ugacaggaaa aaguauacaa      960

ccagaaaacc uugaauauag gauuauguua aguguucacg ggagucaaca uagugggaug     1020

auugucaaug auacaggaca ugaaacagac gaaaauagag caaaagucga aauaacgcca     1080

aacaguccaa gagcugaagc gacauuagga ggauuuggau cauuaggacu agauugugaa     1140

ccaagaacug ggcuagacuu uucagacuua uacuaucuaa caaugaauaa uaagcacugg     1200

uuaguucaua aagaaugguu ucaugacaua ccacuaccuu ggcaugcagg agcagauacu     1260

ggaacuccgc auuggaauaa uaaagaagca uuaguagaau ucaaagaugc acaugcaaaa     1320

cgacagacag uagucguacu uggaagucaa gaaggagcgg ugcauacagc cuuagcagga     1380

gcauuagaag cagagaugga cggcgcuaaa gguagacuua gcagcggaca ucuaaaaugu     1440

agacuaaaaa uggauaaacu aagacuaaaa ggaguaucau auucauuaug uaccgcagcg     1500

uucacauuua caaaaauacc agcggaaaca uuacauggaa cuguaacagu agaaguacaa     1560

uaugcaggaa cugauggacc auguaaagua ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacacccg uaggaagacu aauaacagca aauccaguaa uaacagaaag uacagaaaau     1680

ucuaaaauga uguuggaauu agacccaccu uuuggagaca guuacauagu aauaggagua     1740

ggagaaaaaa aaauaacaca ccauuggcau aggaguggaa gcaccauagg aaaagcauuu     1800

gaagcuacug uaagaggagc gaaacgaaug gcuguucuag gagauacggc uugggacuuu     1860

ggaaguguag gaggagcguu aaauagucuc ggcaaaggaa uucaucaaau auucggagca     1920

gcauuuaaau cacuauuugg uggaauguca ugguuuaguc aaauauugau aggaacacua     1980

uuaauguggu uaggauuaaa uacuaaaaac ggcaguauau cacuaaugug cuuagcauua     2040

gguggaguuu uaauauuccu aaguacagca guaagugcag auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4752
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4752
gggagaaagc uuaccaugac aucaguagga auuguugguu uacuauuaac aacagcaaug       60

gcagccgcag uaacacgccg aggcucggcu uacuauaugu auuuagauag aaacgaugca      120

ggagaagcaa uaucauuucc aaccacacug gguaugaaua aaugcuauau ccaaauaaug      180

gaucuaggac auaugugcga cgcaacaaug ucauaugaau guccaaugcu agaugaagga      240

gucgaaccag augauguaga uugcuggugu aauaccacaa guaccugggu aguauauggc      300

acaugucauc auaagaaagg cgaagcaaga agaaguagac gagcaguaac auuaccauca      360

cauucaacaa gaaaacuuca aacaagaagc caaacuuggc uagaaagcag agaauauaca      420

aaacauuuaa uaagagugga aaauuggaua uucagaaauc cuggcuuugc auuagcugca      480

gcggcuauag cuugguuacu uggaucuagu acuucacaga aaguaauaua ucuaguaaug      540

auacuauuaa uagcaccagc uuacaguauc cgaugcauag gaguaucgaa cagagauuuu      600

guagaaggga ugucaggugg aacaugggua gauauaguau uagaacacgg cggaugcgua      660

acaguaaugg cacaagacaa accaacagua gacauagaac uuguaacaac aacaguauca      720

aauauggcag aaguuagauc auacugcuau gaagcaucaa uaucagacau ggcuucagau      780

aguagauguc caacacaagg agaagccuac uuagauaaac aguccgauac ucaauaugua      840

uguaaaagaa cccuaguaga ccgcgguugg ggaaaugguu gcggacuauu cggaaaagga      900

agucuaguaa ccugugcaaa auuugcaugu aguaagaaaa ugaccggaaa aaguauucaa      960

ccagaaaauu uagaauauag aauaauguua aguguacaug gcucucaaca uucaggaaug     1020

auagucaaug auacagguca ugaaacagau gaaaauagag cuaaagucga aauaacacca     1080

aauucaccac gugcugaagc aacacuagga ggauuuggau cacucggauu agauugugaa     1140

ccaaggacag gcuuggauuu cucagauuua uauuaucuaa cuaugaacaa caaacauugg     1200

uuaguccaua aagaaugguu ucacgauaua ccucuaccau ggcaugcugg ugcagauaca     1260

ggaacacccc auuggaauaa uaaagaagcu cuagucgaau uuaaagaugc acacgcaaaa     1320

cgccagacag uaguuguacu aggaagccaa gaaggagcag uacacacagc ucuagcagga     1380

gcuuuagaag cagaaaugga uggugcaaaa ggaagacuaa gcucaggaca ucugaaaugc     1440

aggcuaaaaa uggauaaauu aagauuaaaa ggaguaagcu auucauuaug uacagcagca     1500

uuuacauuca caaagauacc agcggaaaca uuacacggga ccguaaccgu agaaguacaa     1560

uaugcaggua cagacggacc auguaaagua ccagcacaaa uggcaguaga caugcaaacc     1620

uuaacaccag uaggucgauu aauaacagcc aauccaguaa uaacagaauc aaccgaaaau     1680

uccaaaauga ugcuagaauu agauccaccu uucggagaua guuauauagu aauaggagua     1740

ggagaaaaga aaaucacaca ucacuggcau agaucuggcu ccacuauagg aaaagcauuu     1800

gaagcuaccg uuagaggagc aaaaagaaug gcugucuuag gagauacugc augggacuuu     1860

ggaaguguug gaggugcacu uaacagccua ggaaaaggaa uacaucaaau auucggugca     1920

gcuuuuaaaa gucucuucgg uggaaugucc ugguucucuc aaauuuuaau agguacacua     1980

cuuauguggc uuggucuaaa caccaaaaau ggaucaauau cauuaaugug ccuggcacua     2040

ggaggaguac uaauauucuu aucaacagcu guaagcgcag auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4753
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4753
gggagaaagc uuaccaugag aggagcagau acaaguguug guauaguagg cuuacuauua       60

acaacagcaa uggccgcugc uguaacacga agaggaagug cauauuacau guacuuagac      120

cgaaacgaug caggagaagc cauaucauuu ccaacaacgu uaggaaugaa caaaugcuac      180

auacagauaa uggaucuagg acauaugugu gacgcaacga ugucauacga auguccaaug      240

uuagaugaag gcguagaacc agaugaugua gauuguuggu gcaacacaac aaguacaugg      300

guaguauaug gcacauguca ccauaaaaaa ggggaagcca gaagaagcag aagagcggua      360

acacuaccuu cacauaguac uagaaaacuu caaacgcgcu cucaaacaug gcuugagagu      420

agagaguaua caaaacauuu aauuagagua gaaaauugga uauuucgaaa ucccgguuuu      480

gcauuagcag cagcagcaau agcauggcug cucggaucau caacuucaca aaaaguuaua      540

uauuuaguaa ugauauuacu aauagcacca gcauacagca uacgaugcau aggaguuuca      600

aacagagauu uuguagaagg aaugucugga ggaaccuggg uagacauugu gcuugagcau      660

ggaggaugcg uaacuguuau ggcacaggac aaaccaacgg uugauaucga auuaguuaca      720

acuacaguau caaauauggc ggagguuaga uccuauuguu augaggcauc uauaucagau      780

auggcaucag auucgagaug uccuacucaa ggugaagcuu aucuagacaa acaaagugac      840

acgcaauaug uuuguaaaag aacuuuaguu gacagaggau ggggaaaugg cugugguuua      900

uuuggaaaag ggaguuuagu aacaugugca aaauuugccu guucuaaaaa aaugacaggc      960

aaaucaauac aaccagaaaa uuuagaauau agaauaaugu uaucaguaca uggaagucaa     1020

cauagcggaa ugauaguuaa ugauacagga cacgaaaccg augaaaaccg ugcuaaaguu     1080

gaaauaacac caaauucacc aagagcagaa gcaacauuag gaggauucgg auccuuagga     1140

cuugacugug aaccaagaac aggacuagac uuuagugacu uauauuauuu gacaaugaau     1200

aauaaacauu gguuaguaca uaaagaaugg uuucacgaua uaccauuacc uuggcacgca     1260

ggagcagaua caggcacacc acauuggaau aacaaagaag cuuuaguaga auucaaagac     1320

gcacacgcaa aacgacaaac uguugucgua cuaggaucac aagagggugc aguacacaca     1380

gcgcuagcug gagcauuaga agcagaaaug gacggagcua aaggacgcuu aucaucaggg     1440

caccuaaaau gucgacuaaa aauggauaaa cuaagguuaa aaggaguauc cuauucccua     1500

uguacagcag cauucacauu cacaaaaaua ccagcagaaa cauuacacgg aacaguuacu     1560

guggaaguac aauaugcagg aacagaugga ccauguaaag uuccagcuca aauggccgua     1620

gacaugcaaa cguuaacacc aguaggaaga cuuauuacag cgaacccagu uauaacagaa     1680

aguacugaaa auaguaaaau gauguuggaa uuagauccuc cauuuggaga uucuuauaua     1740

guaaucggcg uaggggaaaa aaagauaaca caucauuggc acagaagcgg aucuacaauc     1800

ggaaaagcau uugaagcaac aguaagaggc gcaaaaagaa uggcaguacu aggagauacc     1860

gcaugggauu uugguagugu aggaggagca cuaaauagcc uaggaaaagg aauacaccaa     1920

auauucgggg cagcauucaa aagcuuauuu gguggaaugu caugguucag ccaaauacua     1980

auaggaacau uauuaaugug gcuuggacuc aacaccaaaa auggaucuau uagccuaaug     2040

ugcuuagcgu uaggaggagu acuaauauuc uuaucaacag caguaagugc agauugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4754
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4754
gggagaaagc uuaccaugcg aggauuauca ucaaggaaac gaagaagcca ugacgucuua       60

acuguacaau ucuuaauacu aggaaugcua uuaaugacag gcgguguaac aagaagagga      120

agugcauacu acauguaucu ugacagaaac gaugcgggug aagccauaag cuuuccaaca      180

accuuaggaa ugaauaaaug uuacauacaa auuauggacu uagggcauau gugcgaugca      240

acaaugaguu augaaugucc uaugcuagau gagggaguag aaccugacga uguagacugu      300

uggugcaaca caacaucaac uugggucguu uaugggacuu gucaccauaa aaaaggugag      360

gcaagaagaa guagaagagc aguaacauua ccuucacauu ccacaagaaa auugcaaaca      420

cgaucacaaa caugguuaga aaguagagaa uacacaaaac accuuauaag agucgaaaau      480

uggauauuuc gaaauccagg cuuugccuug gcugcugcag caauugccug gcuauuaggu      540

aguucuacua gucaaaaagu gauauauuua gugaugauuu uauugauagc accggcauau      600

agcauaagau guauuggcgu aaguaauaga gauuuuguug aagguaugag uggugguacc      660

uggguagaua uaguccuaga acauggagga uguguaacag uaauggcaca agauaaacca      720

acaguagaua ucgaacuagu aacuacaacu gucucaaaca uggcagaggu uagaaguuau      780

uguuacgaag caaguauaag ugauauggca agcgauucuc gauguccaac acaaggugaa      840

gcuuacuuag auaaacaauc agauacacaa uauguaugua aaagaacacu aguugacaga      900

ggauggggua auggaugugg acuauuugga aaaggauccu uagucacuug cgcuaaauuu      960

gccuguagca aaaaaaugac ggguaaauca auacaacccg aaaauuuaga auaccgaauu     1020

auguugagug uucauggauc ucaacauuca gguaugauug uaaaugauac aggacaugaa     1080

acagaugaaa accgagcaaa aguagaaauc acuccaaaca gcccuagagc agaggcaacc     1140

cuuggaggau ucggaaguuu aggccuagau ugugaaccaa gaacaggauu agauuuuagu     1200

gacuuauacu accuaacaau gaauaauaaa cauugguuag uacauaaaga augguuucau     1260

gauauacccu uaccuuggca ugcuggagca gacacaggaa cgccacauug gaauaacaaa     1320

gaagcacuag uugaauuuaa agaugcacau gcaaaaagac aaacaguagu aguauuagga     1380

agucaagagg gagcagugca cacagcuuua gcaggcgcac uagaagcuga aauggacggu     1440

gcaaaaggua gacuaucaag uggacacuua aaauguagac uaaaaaugga caaauuaaga     1500

uuaaaagggg ugucauauuc uuuauguaca gcugcauuua cauucacuaa aauuccagca     1560

gaaacacuac auggaacagu aacaguggaa guacaauaug caggaacaga uggaccaugc     1620

aaagugccug cacaaauggc aguagauaug caaaccuuaa ccccaguagg aagauuaaua     1680

acggcaaacc cgguaauaac cgaaucaacu gaaaacagua aaaugauguu ggaacuagau     1740

ccaccauuug gagauucaua uauaguuaua ggagugggag aaaaaaaaau aacccaucau     1800

uggcauagaa guggaagcac aauaggaaag gcauucgaag cuacagucag aggagcaaaa     1860

agaauggcug uucuuggaga uacagcaugg gauuuuggua guguuggugg agcuuuaaau     1920

agccuuggaa aaggaauuca ucaaauauuu ggagcagcau ucaaaagcuu auuuggagga     1980

augucuuggu uuucacaaau auugauagga acacuuuuaa ugugguuagg auugaacaca     2040

aagaacggaa guauaucuuu aauguguuua gcauuaggug guguucuaau uuuucuauca     2100

accgcaguaa gugcagauug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4755
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4755
gggagaaagc uuaccaugag aggauugagu aguagaaaac gacgaaguca cgauguucua       60

acaguacaau uucuaauuuu agguaugcua uugaugacag gagcaguaac aagacgagga      120

ucagcauauu auauguauuu agauagaaau gaugcaggag aagcaauaag uuuuccaacu      180

acuuuaggaa ugaacaaaug uuauauucaa auaauggauu uaggccauau gugugaugcu      240

acaaugaguu augaaugccc aauguuagac gaaggaguag aaccagacga cguagauugu      300

ugguguaaua caacaucaac auggguagug uauggcacau gucaucacaa gaaaggagaa      360

gcgagaagaa guagacgagc aguuacacua ccaagucaua gcacgagaaa auuacaaaca      420

agaucacaga ccugguuaga aucaagagaa uacacuaaac accugauaag aguagaaaau      480

uggauuuuua gaaaucccgg auuugcacua gcagcugcag cuauagcuug gcuauuaggu      540

aguuccacua gccaaaaagu aauuuaccua guaaugauau uacuaauagc accagcauau      600

aguauaagau gcauaggagu gucaaauaga gauuuuguag aaggaaugag cggugguaca      660

uggguggaua uagucuuaga acauggagga uguguaaccg uaauggcaca agacaaacca      720

acaguugaua ucgaacuugu aacaacaacg guaucaaaua uggcugaagu aagaucauac      780

uguuaugaag caaguauauc agauauggca agugacucaa gaugcccaac acaaggagaa      840

gcauaucuag auaaacaauc agacacacaa uaugugugca aaagaacauu aguagacaga      900

ggauggggaa auggaugugg acuauucgga aaagggagcu uaguaacaug cgcuaaauuc      960

gcgugcagua agaaaaugac aggaaaaagu auacaaccag aaaauuuaga auauagaaua     1020

augcuaucag uacacggauc gcaacauuca gguaugauag uaaacgauac aggacaugaa     1080

acagaugaaa accgagcuaa aguggaaaua acgccaaauu cacccagagc cgaagcaaca     1140

uuaggggguu ucggaaguuu gggauuggau ugcgaaccua gaacgggguu agauuuuuca     1200

gauuuauauu accuaacaau gaauaauaaa cauuggcucg uacacaaaga augguuucau     1260

gauauaccuu uaccauggca ugcaggagcc gauacuggua cuccacauug gaauaauaaa     1320

gaagcccuag uagaauuuaa ggaugcucac gcaaaaagac aaacaguugu gguucuagga     1380

agucaagaag gagcgguaca uacagcauua gcaggcgcau uagaagcuga aauggaugga     1440

gcgaaagguc gacugaguuc aggacaucua aaauguagau uaaagaugga uaaacuaaga     1500

cuaaaaggag uaucauauuc auuauguacu gcagcuuuua cguuuaccaa gauuccagca     1560

gaaacacuac auggaacugu uacaguagaa guucaauaug cuggaacaga cggaccaugc     1620

aaaguacccg cacagauggc uguagacaug cagacauuaa ccccaguagg acgacuaaua     1680

acggcuaacc cagucaucac ugaaucaacu gaaaauucga aaaugaugcu agaacuagac     1740

ccaccuuuug gagacaguua uauaguuauu ggaguuggag aaaagaaaau aacacaucac     1800

uggcauagaa gcggaaguac aauagguaaa gcauuugaag caacaguacg cggugcaaaa     1860

agaauggcag uuuuaggaga uacugcaugg gauuucggau cuguaggugg agcacuaaac     1920

ucucuuggaa aagguauuca ucaaauauuu ggagccgcuu uuaaaaguuu guuuggagga     1980

augagcuggu uuucacagau auuaaucgga accuuacuaa uguggcuagg ucuaaauacc     2040

aaaaauggau caauaaguuu aauguguuua gcacuuggag gaguauuaau auuccugagc     2100

acagcaguaa gugcggacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4756
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4756
gggagaaagc uuaccaugcg aauaauaaac gccagaaaag aaaaaaagag aagaggggca       60

gauacaucug uggguaucgu aggauuauua cuuacaaccg cuauggcggc ggaaguaaca      120

agaagaggau cagccuacua uauguaccua gacagaaaug augcugguga agcaauaucu      180

uucccuacaa cuuuaggaau gaauaaaugu uacauccaaa uaauggacuu aggacauaug      240

ugcgaugcaa cuaugucgua ugaauguccu auguuagaug aaggaguaga accggaugac      300

guugauugcu gguguaacac aacaagcacc uggguaguau acgggacaug ccaucauaaa      360

aaaggggaag caagacgaag ccgaagagcu guuacuuuac caagucauuc aacgagaaaa      420

cuucaaacga gaucucaaac auggcuugaa uccagagaau auacaaaaca uuuaaucagg      480

guagaaaacu ggauuuuuag aaauccagga uucgcauuag cagccgcggc gauagcaugg      540

cugcuagggu caagcacauc ucaaaaagua auauaucuag uaaugauacu auuaauagcc      600

ccagcauauu caauaagaug uauaggagua aguaacagag auuuuguaga aggaaugucu      660

gguggaaccu ggguagauau aguauuagaa cauggaggau guguaacugu cauggcacaa      720

gauaaaccca ccguagacau agaacuggua acaacaacag uaucaaauau ggcugaagua      780

cguaguuauu gcuaugaagc aucaauauca gauauggccu cagauucacg auguccuacc      840

caaggagaag ccuacuuaga uaaacaaucc gacacacaau acguguguaa aagaacauua      900

gucgauagag gcuggggaaa uggcugugga uuauuuggaa aaggaucacu aguaacaugu      960

gcaaaauucg cgugcucaaa aaaaaugaca ggaaaaucca uucaaccuga aaacuuagaa     1020

uauagaauua uguuaagugu acacggguca caacauagcg gaaugauagu aaacgacaca     1080

ggacaugaaa cagacgagaa cagagccaaa guagaaauaa caccaaauag cccaagagcu     1140

gaagcaacac uagguggauu cggaaguuua ggauuagacu gugagccacg aacugguuua     1200

gacuucagcg aucuguacua uuuaacaaug aacaauaaac acuggcuagu acauaaagaa     1260

ugguuccaug auauaccauu gccauggcau gcaggagcag auacaggaac accacauugg     1320

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaaagaca gacgguagua     1380

guuuuaggau cacaagaagg agcuguccau acagcucucg cgggcgcacu ugaagcugaa     1440

auggacggag cgaaaggcag acuaucaucc ggucacuuaa aauguagacu aaaaauggau     1500

aaacuaagac uuaaaggagu aucauauuca uuauguacug cugcauuuac auuuacuaaa     1560

auaccagcag aaacacuuca uggaacggua acuguagaag uacaauaugc ugggacagau     1620

ggaccaugca aaguaccagc acaaauggca guugacaugc aaacauugac accaguugga     1680

agauuaauua cagcaaaucc uguaauaaca gaaagcacag agaauucaaa aaugauguua     1740

gaauuagauc caccguucgg agauagcuau auaguaauug gaguaggaga aaaaaaaaua     1800

acacaucauu ggcacagauc aggaucaacu auaggcaaag cuuuugaagc aacaguuagg     1860

ggcgcaaaaa ggauggcagu auuaggagau acagcauggg acuuuggaag cguaggaggu     1920

gccuuaaaua gucuuggaaa aggaauacau caaauuuuug gagcagcauu uaaaagucua     1980

uuugguggaa ugaguugguu uagucaaauu cuaauaggaa cucuauuaau guggcuagga     2040

cuaaauacaa aaaacggguc aauaucauug augugucuug cauuaggagg agugcuaaua     2100

uucuuaagua cagccguaag cgcuugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4757
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4757
gggagaaagc uuaccaugau aaacgcaaga aaggaaaaaa aaagaagagg ugccgauaca       60

ucaguaggaa uuguugguuu guuguuaaca acagcaaugg cagcagaagu uacgagaaga      120

ggaucagcau acuacaugua ucuugaccga aacgaugcag gcgaagcaau aagcuuuccc      180

acaacauuag gaaugaauaa auguuauauu cagauaaugg auuuaggaca uaugugcgau      240

gcuacgauga guuaugaaug ucccaugcua gaugaaggag uugaaccaga ugauguggau      300

uguuggugua auacaacauc uacgugggua guauacggaa caugccauca uaaaaaaggc      360

gaagcaagaa gaucaagacg agcgguaaca uuaccuucac auucaacuag aaaacuccaa      420

acgagaagcc agacauggcu ggaaucaaga gaauauacaa aacaucuuau uagaguagaa      480

aacuggauau uucgaaaucc agguuucgcu uuagcagcag cagcaauagc auggcuacua      540

ggaucuucua caucacaaaa aguaauauau uuaguaauga uuuuguuaau agcaccagca      600

uacaguauaa gguguauagg uguaaguaau agagacuuug uagaaggaau gagcggagga      660

acguggguag acauaguauu agaacaugga ggauguguua caguaauggc acaggauaaa      720

ccuaccguag acauagaauu ggucacaacu acagucagca acauggcuga aguacgaagu      780

uauugcuaug aagcuucaau uagugauaug gcaucagaua gcaggugucc aacacaaggu      840

gaagcauauu uagauaaaca aagcgauaca caauauguau guaaaagaac auuaguagac      900

agaggauggg ggaaugguug uggacuauuu gguaaaggaa gcuuaguaac augcgcuaaa      960

uuugcaugcu caaaaaaaau gacuggcaaa agcauacaac cagaaaauuu agaguaucga     1020

auaauguuau cagugcacgg aagucaacac ucaggaauga ucgugaauga cacaggccau     1080

gaaacggaug aaaacagagc aaaaguagaa auaacuccaa acucaccaag agcagaagca     1140

acacuaggag gauuuggauc auuaggacua gacugcgaac cuagaacagg acuagauuuc     1200

ucagauuuau acuaucuuac aaugaauaac aaacauuggu uaguccauaa agaaugguuu     1260

caugauauuc cauuaccaug gcaugcagga gcugauacag gaacgcccca uuggaauaau     1320

aaggaagcuu uaguagaauu uaaagaugca cacgcaaaaa gacaaacagu aguuguauua     1380

gggagucaag agggugcagu acacacagca uuagcuggug cgcuagaagc agaaauggac     1440

ggggcaaaag gaagacuuag uucuggacau uuaaaaugca gacuaaaaau ggauaaauua     1500

agauuaaaag gcguaaguua uaguuuaugc acugcugcau ucacauuuac aaaaauacca     1560

gcagaaacau uacauggaac gguaacagua gaaguacaau augcaggaac ugauggacca     1620

ugcaaagugc ccgcacaaau ggcaguggau augcaaacgc ucacaccagu aggaagauua     1680

auaacagcua auccagucau aacagaauca acugaaaauu caaagaugau guuagaauua     1740

gacccaccuu uuggagauuc auacauugua auaggaguag gagaaaaaaa aauaacacau     1800

cauuggcaua gaaguggauc aacaauagga aaagcauuug aagcaacagu acgaggggcc     1860

aaaagaaugg caguacuagg agauacagca ugggacuuug gaucgguagg gggagcucua     1920

aauuccuuag gaaaaggcau acaucaaaua uuuggagcgg cguucaaauc uuuauuuggc     1980

gguaugucuu gguucucuca aauuuuaaua ggaacccuuc uaaugugguu agguuuaaau     2040

acuaaaaaug gaucuaucag cuuaaugugc uuagcccuag gagguguauu aauuuuuuua     2100

ucaacagcag uuucagcuug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4758
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4758
gggagaaagc uuaccaugau aauaaaugcu aggaaagaaa aaaaaagacg gggagcagau       60

acaaguguag gaauaguagg auuacuacua acaaccgcca uggcagcaga agucacaaga      120

agagguucag cauacuauau guacuuagac aggaacgaug caggagaagc cauuaguuuu      180

ccgacaacac uagguaugaa caaauguuau auacaaauaa uggauuuagg acauaugugc      240

gaugcaacaa ugucauauga augcccaaug uuagacgaag gaguagaacc agaugauguc      300

gauuguuggu gcaauacaac aucaacaugg guaguauacg guacguguca ucacaaaaaa      360

ggcgaagcac guagaagucg aagagcagua acucuaccaa gccauaguac aagaaaacua      420

caaacucgau cacaaacgug guuagaaagc agggaauaua cuaaacacuu aauaagagua      480

gaaaacugga uauuucgaaa uccagguuuu gcuuuagcug cagcagcaau agcauggcua      540

uuagggucau ccacgucaca aaaaguaaua uauuuaguaa ugauauuacu aauagcacca      600

gcguacucca uaagauguau aggaguuagc aauagagacu ucguugaagg aaugucuggu      660

ggaacauggg uugauauagu ccuugaacac ggaggaugug uaacggucau ggcacaagau      720

aaaccaacug uugauauaga acuaguaacc acaacaguaa gcaauauggc agaagucaga      780

ucauauuguu acgaagccag uauaagcgau auggcaucag auucaagaug uccaacucag      840

ggugaagcau auuuggauaa acaaucagau acacaauaug ucugcaaacg caccuuagua      900

gauagagggu gggguaaugg uuguggacua uuuggaaaag gaucacuagu aacaugcgca      960

aaauucgcau guucaaaaaa aaugacaggg aaauccauac aaccagaaaa uuuagaauac     1020

agaauaaugc uaucagucca uggaucacaa cauaguggua ugauuguuaa ugauacaggu     1080

cacgaaacag augaaaauag agcaaaagua gaaauaacac caaauagucc aagagcagaa     1140

gcaacauugg gaggauuugg aucucuaggu cuagauugug aaccuagaac aggacuggac     1200

uuuagugacc uuuauuacuu aacaaugaac aauaaacauu ggcuaguaca uaaagagugg     1260

uuccaugaua uaccacuacc auggcaugcc ggagcagaua caggaacucc ccacuggaau     1320

aauaaagaag cucuaguaga auuuaaagac gcacaugcca aaagacaaac aguagucgua     1380

uuaggauccc aagaaggagc cguacauaca gcacuagccg gagcauuaga agcagaaaug     1440

gauggagcaa aaggacgacu gagcucaggu caucuaaaau guagauuaaa aauggacaaa     1500

uuaagauuaa aagggguguc auacagucua uguacagccg cauuuacuuu cacaaaaaua     1560

ccagcugaaa cauuacacgg cacagugacc guugaaguac aauaugcagg aacagauggu     1620

ccaugcaaag uacccgcaca aauggcaguc gauaugcaaa cauuaacacc aguaggaaga     1680

uuaaucacag caaauccagu aauaacagaa aguaccgaaa auucaaaaau gauguuggaa     1740

uuagauccac cauuuggaga uaguuauaua guaauaggag uuggagaaaa aaaaaucacc     1800

caccacuggc acagaagugg caguacaaua gguaaagcgu ucgaagcaac ugucagagga     1860

gcaaaaagaa uggccguacu uggagacaca gcaugggauu uuggaucagu uggcggcgcu     1920

cugaacuccc uaggaaaagg aauccaucaa auauuuggag cagcauuuaa aaguuuauuu     1980

ggagguauga guugguucuc acaaauacua auuggaacac uucuaaugug gcuaggacua     2040

aauacaaaga acggcaguau aucauugaug uguuuagcgc uaggaggcgu acuaaucuuu     2100

cuaagcacag cgguuucagc uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4759
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4759
gggagaaagc uuaccaugaa ugcaagaaaa gaaaagaaaa gaagaggugc cgauacaucc       60

guagguauag uuggcuuauu auuaacaaca gccauggcag cugaagugac uagaagaggu      120

ucagcauauu acauguaucu agauagaaau gacgcaggag aagcaauaag uuuucccacu      180

acauuaggaa ugaauaaaug uuauauacaa auaauggauc uuggacacau gugcgaugcu      240

acaaugaguu augaaugucc aauguuagau gaaggagugg aaccagauga uguagauugu      300

ugguguaaca cgacaucaac uuggguaguu uauggaacau gucaucauaa gaaaggagaa      360

gcaagacgau caagacgagc aguaacacuc ccaagccacu cuacaagaaa auuacagaca      420

agaucacaaa caugguuaga aucccgagaa uacacaaaac accuaauuag aguagagaac      480

uggauuuuua ggaauccagg auuugcauua gcggccgcug caauagcaug gcuacuagga      540

ucaaguacaa gucaaaaagu caucuauuua guaaugauau uauuaauagc cccugcauac      600

ucaauaagau guauuggcgu aaguaauaga gacuuuguag aagggaugag uggaggaacu      660

uggguagaua uaguauuaga gcauggagga uguguaacag uaauggcaca agacaaacca      720

acuguagaua uugaguuagu aacaacaacu gugaguaaca uggcagaagu aagauccuau      780

ugcuacgaag cauccauuuc agauauggcu ucagauagua gauguccaac ucaaggagaa      840

gccuaucuag acaaacaaag ugacacacaa uauguuugua aaagaacauu aguagauaga      900

ggauggggua auggaugugg cuuauucgga aaagguucau uaguuacaug cgcaaaauuu      960

gcuuguucaa agaaaaugac cggcaaauca auucaacccg aaaauuuaga auauagaaua     1020

augcucagug uacacggaag ccaacauuca gguaugauag uaaacgauac ugggcaugaa     1080

acagacgaaa auagagcuaa aguagaaauu acaccaaauu ccccaagagc agaagcuaca     1140

uugggaggau uugggagucu aggacuugau ugugaaccaa gaacaggucu agacuucagu     1200

gaucuguacu aucuuacaau gaauaacaaa cauuggcuug uccauaaaga augguuucac     1260

gacauaccac uaccauggca ugcaggagca gauacuggaa cgccacacug gaauaacaaa     1320

gaagcauuag uugaauuuaa agaugcacau gcgaaaagac aaacaguagu aguuuuaggu     1380

agccaagaag gagcuguaca uacagcauua gcaggagcau uagaagcaga aauggaugga     1440

gcgaaaggua gauuaucauc aggacaucua aaaugucgac uaaaaaugga uaaauuaaga     1500

cucaaaggag uuucauacuc auuauguaca gcagccuuua cuuucaccaa aauaccagca     1560

gaaacacugc auggaacugu aacaguugaa guacaauacg caggaacaga uggaccaugu     1620

aaaguaccag cgcagauggc aguagauaug caaacacuca caccuguagg uagacuaauc     1680

acagcaaauc cgguaauaac agaaaguaca gaaaauucua aaaugaugcu agaacuagau     1740

ccgccauucg gagacagcua cauaguaaua ggaguuggug agaagaaaau aacacaccau     1800

uggcacagau caggaaguac aauaggaaaa gcauuugaag caaccguaag aggagccaaa     1860

aggauggcag ucuuaggaga uacagcaugg gauuuuggaa guguaggggg ugccuuaaau     1920

uccuugggua aagguauaca ucaaauuuuu ggagcagcau uuaaaucauu auuuggcggc     1980

auguccuggu ucucacaaau acugauagga acucuauuaa uguggcuugg cuuaaauacg     2040

aaaaauggaa guauaucauu gaugugccua gcauuaggcg gaguucuaau auuuuuaagu     2100

acagcaguau cagcaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4760
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4760
gggagaaagc uuaccauggc aagaaaagaa aagaaaagaa gaggagcgga cacaagugug       60

ggaauaguag gacuauuauu aacaacagcu auggcagcag agguaacgag gagaggauca      120

gcauauuaua uguaucuaga ccgaaaugau gcaggagaag cuauuucauu cccaacgacu      180

cuaggaauga acaaaugcua cauacaaaua auggacuuag gacauaugug ugacgccaca      240

augaguuaug aaugcccaau gcuagaugaa ggcguagaac cagaugacgu agauuguugg      300

ugcaauacaa ccucgaccug gguagucuac ggaacauguc accauaagaa aggagaagca      360

agaaggucaa gacgagccgu cacuuuaccu agccauucaa cacgaaaacu acaaacaaga      420

ucacaaacau ggcuagaaag uagagaauau accaaacauu uaauaagagu agaaaauugg      480

auauucagaa acccaggauu cgcauuagcu gcagcagcua uagcuuggcu auuaggcagc      540

ucaacaaguc aaaaagugau auaucuggua augauacuac uuauagcucc ugcauacuca      600

auuagaugua uaggcguauc aaauagagau uuuguugaag gaaugagcgg aggaacaugg      660

guagacauag uauuagaaca cgguggaugu gugacaguua uggcacaaga uaaaccaaca      720

guagauauug agcuaguuac uacaacagua ucaaacaugg ccgaaguaag aaguuacugc      780

uacgaagcau caauaaguga uauggcauca gauagcagau guccaaccca aggugaagca      840

uauuuagaua aacaaucaga uacucaauau guuuguaaaa gaaccuuagu agauagagga      900

uggggaaaug gauguggguu auuuggaaaa ggaaguuuag uaacaugcgc uaaauuugcc      960

uguucgaaga aaaugacagg aaaaucuauu caaccagaaa aucuagaaua ccgaauaaug     1020

cuuagcgugc auggaaguca acacagcgga augauaguca acgauacugg acaugaaacg     1080

gaugaaaaca gagcaaaagu ugaaauaaca ccgaauaguc ccagagcaga ggcaacauua     1140

ggaggauuug guuccuuagg acuagacugc gagccaagaa caggccuaga uuucagcgau     1200

uuauauuauc uaacuaugaa caauaaacac ugguuggucc auaaagaaug guuucaugau     1260

auacccuuac cuuggcaugc uggugcagau acaggaacac cacauuggaa uaauaaagaa     1320

gcacuuguag aauuuaaaga ugcacaugca aaaagacaaa caguaguagu cuuaggaucu     1380

caagagggag caguacacac ggcacuugcu ggggcauuag aagcagaaau ggauggagcu     1440

aaaggucgau uaaguagugg acacuuaaaa ugccgauuaa aaauggacaa auuaagauua     1500

aaaggaguau cauauucacu augcacagcu gcuuucacau uuacaaaaau accagcugaa     1560

acacuacaug gaacaguaac aguagaagua caauacgcag gaacagaugg accguguaaa     1620

guaccagcac aaauggcagu ggauaugcaa acacuuacgc caguaggaag auuaauaaca     1680

gcuaaucccg uaauuacuga aaguacagaa aauucuaaaa ugaugcuaga acuagaccca     1740

ccuuucggug auaguuauau aguaauagga guaggagaaa agaaaaucac acaucauugg     1800

caucgaucag gaaguacaau aggaaaagca uuugaagcua caguaagagg cgcuaaacga     1860

auggcaguuc ugggagauac ugcaugggau uuuggaagug uaggaggagc acuaaacuca     1920

uuaggaaaag gcauccacca aauauuugga gcugcauuua aaagucuauu cggagguaug     1980

ucaugguuca gucaaauauu aauaggaacg uuauugaugu gguuaggcuu aaauacuaaa     2040

aacgguucaa uuucacuaau gugucuagca cuaggaggag uauuaauauu cuuaaguacg     2100

gcaguaagug caugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4761
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4761
gggagaaagc uuaccaugcg gaaagaaaaa aaaagaagag gugcugauac aaguguagga       60

aucguaggac uauuauuaac aacagccaug gcagccgaag uaacaaggag aggcuccgca      120

uauuauaugu aucuagaucg aaacgaugcu ggugaagcaa uaucuuuucc aacaacacua      180

ggaaugaaua aauguuauau acaaauuaug gaucuagggc auaugugcga ugcaacuaug      240

aguuaugaau gcccaauguu agacgagggu guagaacccg augauguuga uuguuggugu      300

aauacaacaa gcacaugggu aguauaugga acaugccacc acaaaaaagg ugaggcaaga      360

agaagccgaa gagcuguaac auuaccauca cauagcacua gaaaauuaca aacgagaagc      420

caaacauggu uagaaucaag agaguacacu aagcaucuaa uuagaguaga aaacuggaua      480

uuuagaaauc caggauucgc acuugcagca gcggcaauag cauggcuauu aggaucauca      540

acaagucaaa aaguaauaua uuuaguuaug auacuuuuaa uagcacccgc auauagcauc      600

agaugcauug gaguaucaaa uagagacuuu gucgaaggua ugucuggagg uaccugggua      660

gauauaguau uagaacaugg uggaugugua acgguaaugg cacaagacaa accaacugug      720

gauauagaac uaguaacuac aacaguaagu aauauggcag agguaagauc uuacugcuau      780

gaagcaucaa uaucagacau ggcaagcgac ucaagaugcc cgacacaagg agaagccuau      840

uuagauaaac aaucggacac gcaauauguu uguaaacgca cuuuaguaga uagaggaugg      900

gguaauggau gugguuuauu uggaaaagga agcuuaguua caugugcuaa auucgcaugu      960

ucaaaaaaaa ugacaggaaa aucaauacaa cccgaaaacu uggaauaucg aauaauguua     1020

agcguacaug guucacaaca cucagguaug auugucaaug acacagguca ugaaacagac     1080

gaaaacagag caaaaguaga aauaacgcca aacucaccua gagcugaagc aacacuaggu     1140

gguuuuggau cucugggacu agauugcgaa ccaagaacag gacucgauuu cagugauuua     1200

uauuacuuaa cuaugaauaa uaaacacugg cuaguccaca aagaaugguu ucaugauaua     1260

ccacugccau ggcacgcagg ugcagauacc gguacacccc auuggaauaa caaagaagca     1320

cuaguggaau uuaaagaugc acaugccaaa cgucaaacag ugguaguuuu aggaagucaa     1380

gaaggagcag uccauacggc ucuagcaggu gcacuagagg cagaaaugga uggggcuaaa     1440

ggaagauuau caaguggaca uuuaaagugu agacuaaaaa uggauaaauu gagauuaaaa     1500

gggguaaguu acucauuaug cacagcugca uuuacauuca caaaaauucc agcagaaaca     1560

cuacauggca caguaacagu agaaguccaa uacgcaggaa ccgauggucc guguaaggua     1620

ccugcacaaa uggcaguaga caugcaaaca uuaacaccag ucggccgacu aauaaccgca     1680

aauccaguaa uuacagaaag uacagaaaau aguaaaauga uguuagaauu agauccacca     1740

uuuggagauu ccuacauugu uauaggagua ggugagaaaa aaauaacgca ucauuggcac     1800

agaaguggaa guacuauagg caaagccuuu gaagcuacag uaagaggggc gaaaagaaug     1860

gcaguauuag gagauacagc uugggauuuu ggaagugucg gcggcgcacu uaauaguuua     1920

ggcaaaggua uucaccaaau auuuggagcg gcauucaaau cacuauucgg aggaaugucu     1980

ugguuuucuc aaauauuaau aggaacauua cuaauguggu uaggacuaaa uaccaaaaau     2040

ggcuccauau cacucaugug uuuagcauua ggagggguac uaauauucuu aagcacagca     2100

guaucagcau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4762
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4762
gggagaaagc uuaccaugaa agaaaaaaaa agaagaggag cagauacaag uguagguaua       60

guaggauugu uacuaacaac agcuauggca gcagaaguaa caagaagagg aagugcauau      120

uauauguacc uagauagaaa ugaugcaggu gaagcaauaa guuuuccaac aacauuggga      180

augaauaaau guuauauaca aauaauggau uuaggacaua ugugugacgc uaccauguca      240

uacgaauguc caaugcuaga ugaaggagua gaaccagaug auguagacug uugguguaac      300

acuacuucaa cauggguagu auauggaaca ugccaucaua aaaaaggaga agcaagaaga      360

ucaagaagag caguaacacu accaucacau ucuacaagaa aauuacaaac acgaagccaa      420

acauggcuug aaucacgaga auauacaaaa caucuaauac guguagaaaa cuggauauuu      480

agaaacccgg gauucgcuuu agcagccgcu gcaauagcau ggcugcuagg uucaucaacc      540

ucacaaaaag ucauauaucu aguuaugaua cuacuaauag cucccgcaua cucaaucaga      600

uguauaggag uaagcaauag agauuuuguu gaaggaaugu caggagguac auggguagau      660

auaguguuag aacauggagg auguguaaca guaauggcgc aagauaaacc aacaguagau      720

auagaauuag uaacaacuac aguaaguaac auggcugagg uuagaaguua cuguuacgag      780

gcgucuauaa gcgauauggc aucagacagu agauguccaa cccaagguga agcauauuua      840

gauaagcaga gugacacaca auauguaugc aaaagaacgu uaguugaucg aggaugggga      900

aauggaugug gauuauuugg uaaaggaagu cuaguaacau gugccaaauu ugcauguagu      960

aaaaaaauga caggaaaaag cauacaaccu gaaaaucuag aauauaggau aauguuaagu     1020

guacauggaa gccaacacuc uggcaugauu guaaaugaca caggucauga aacggacgag     1080

aacagagcga aaguagaaau aaccccgaau agccccagag cugaagcaac auuaggggga     1140

uuugguaguc uagguuuaga uugugaacca agaacagggu uagauuuuag ugauuuauau     1200

uacuugacaa ugaacaacaa acauuggcua guacacaaag aaugguuuca cgauauacca     1260

cuacccuggc augcuggagc ggacacaggu acaccccacu ggaauaauaa agaagcucua     1320

gucgaauuua aagaugcaca ugcaaaaaga caaacaguag uaguuuuagg aucacaagaa     1380

ggagcaguac auacggcacu agcaggagcg uuagaagcag aaauggaugg ggcuaaaggg     1440

cgauuaucau caggacacuu aaaaugucga uuaaaaaugg auaaacuaag auuaaaagga     1500

guaucauaua guuuaugcac agcagcauuu acuuuuacaa aaauaccagc cgaaacacuu     1560

caugguacug uaacagugga aguccaguau gcuggaaccg auggaccaug uaaagugcca     1620

gcgcaaaugg caguagauau gcaaacauua accccgguag gcagacuuau aacugccaac     1680

ccuguaauaa cagaaucaac ggaaaacuca aaaaugaugc uugaauuaga uccgccauuu     1740

ggagacagcu acauuguaau agguguagga gaaaaaaaaa uaacacacca cuggcauagg     1800

ucaggaucga ccauugguaa agcguuugaa gcaacaguac gaggagcuaa aagaauggca     1860

guacuuggag acacagcuug ggauuuuggu ucuguaggag gugcauuaaa cagccuagga     1920

aaaggaauac aucaaauauu uggagcagcu uuuaaaaguc uauuuggugg aaugaguugg     1980

uuuagccaaa uauuaauugg aacguuauua augugguuag gguuaaauac aaagaacggu     2040

aguauuaguc uuaugugcuu agcuuuagga ggaguacuaa uauuuuuaag cacagccgua     2100

ucagcaugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  4763
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4763
gggagaaagc uuaccaugga aaagaaaaga agaggggcgg auacauccgu aggaauaguu       60

ggacuauuau uaacaacugc aauggccgca gaaguaacac guagaggauc agcuuauuau      120

auguaucuag auagaaacga ugcaggagaa gcuauaaguu uuccaacaac acuaggaaug      180

aauaaauguu auauacaaau aauggaccuc ggccacaugu gugacgcuac aaugucuuau      240

gaauguccca uguuagauga aggagucgaa ccagaugacg uagauugcug guguaauaca      300

acaucuacuu ggguaguaua ugguacaugu caucacaaga aaggugaagc aagaagauca      360

cgaagggcag uaacuuuacc aucucacagc acuagaaaau uacaaacuag aagccaaaca      420

ugguuagaau cuagagaaua uacaaaacau uuaauacgag uagaaaauug gauauuuaga      480

aacccugguu uugccuuggc agccgccgcu auagcauggu uacuaggauc aucaacaagc      540

caaaaaguaa uauaccuagu uaugauacua cuaauugcac cagcauauuc aauuaggugu      600

auaggaguua gcaauagaga uuuuguagaa gguaugucag guggaacaug gguagacauu      660

guauuagaac acggagguug ugugacugua auggcgcaag auaaaccaac aguagacaua      720

gaauuaguaa cuacaacugu aaguaacaug gcagagguaa gaucguauug cuaugaagca      780

aguauaagug acauggcuuc agauucaaga uguccaacac aaggugaggc auaucuagac      840

aaacaaucag acacacaaua cguauguaaa agaacuuuag uagaccgcgg cuggggaaac      900

ggaugcggac uauuugguaa aggaaguuua guaacaugcg caaaauucgc auguucuaag      960

aaaaugacag gaaaaucaau acaaccugaa aaucuagaau auagaauaau gcuaagugua     1020

cauggaaguc aacauucagg aaugauugug aacgacacug gacaugaaac cgaugaaaau     1080

agagcaaaag uagaaauaac accaaauucg ccaagagcag aagcaacucu uggggguuuu     1140

ggaucauugg gauuagauug cgagccuaga acaggauuag auuucagcga ucuuuauuac     1200

uugacaauga auaauaaaca uugguuagua cauaaagaau gguuccauga uauaccacua     1260

ccauggcaug cgggagcaga uacugguaca ccucauugga acaauaaaga agcacuggua     1320

gaguucaaag augcccaugc aaaacgacaa acaguaguag uauuaggguc acaagaagga     1380

gccguucaca cagcacucgc uggcgcauua gaagccgaaa uggauggcgc aaaaggaaga     1440

uugucuucag gacaucuaaa augccgauua aaaauggaca aauuaagacu aaaaggggua     1500

agcuauucau uauguacagc ggcauuuaca uuuacaaaaa uaccugcaga aacucuacau     1560

ggaacaguaa caguggaagu acaauaugca gguacugacg guccuuguaa aguaccagca     1620

caaauggcag uagacaugca aacuuuaaca ccaguaggga gauuaauuac ugcgaaucca     1680

guuauaacag aaaguacaga aaauucaaaa augauguuag aauuagaucc accauucgga     1740

gauagcuaua uaguaauagg uguaggagaa aagaaaauaa cucaucacug gcacagaagu     1800

ggauccacaa uaggaaaagc auucgaagca acaguaagag gcgcaaaaag aauggcagua     1860

uuaggcgaca cugcauggga uuuuggguca guaggaggag cauugaauag ucucgguaaa     1920

gguauccauc aaauauuugg agccgccuuu aaaaguuuau ucggaggaau gucuugguuu     1980

ucucaaaucu uaauagguac acuacuaaug uggcucggac uaaauacgaa aaauggcucg     2040

auaucacuaa uguguuuagc ucuaggaggu guacuaauau uuuuaucaac agcagugagc     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  4764
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4764
gggagaaagc uuaccaugaa gaaaagaagg ggagcagaua caaguguagg aauaguagga       60

cuauuacuga caacagcaau ggccgcggaa guaacaagaa gaggaucagc uuacuacaug      120

uacuuagacc gaaaugaugc aggagaagca auaucguuuc caaccacauu aggaaugaau      180

aaauguuaua uacaaauaau ggauuuagga cauaugugug acgcaacaau gagcuaugaa      240

ugcccaaugc uagaugaagg agucgaaccu gaugauguag auuguuggug caacacaacu      300

agcacuuggg uaguauacgg aacaugucac cauaagaaag gugaagcuag acgaaguagg      360

agagcuguaa cauuacccag ucauagcaca agaaaauuac agacaagaag ccaaacaugg      420

uuagaaucaa gagaauacac aaaacaccua auaagaguag aaaauuggau auuuagaaau      480

ccaggcuucg cucuggcagc agcagcgaua gccuggcuac uaggaaguuc gacaagucaa      540

aaaguaauau auuuaguuau gauuuuacug auugcuccag cauauaguau aagauguaua      600

ggagucagua accgagauuu uguagaaggc augucaggag gaacaugggu agauauugua      660

uuagaacaug gagguugugu cacaguaaug gcacaagaca aaccaacagu agauauagaa      720

uuaguaacaa cuacaguaag uaauauggca gagguaagau cauauuguua ugaagcaucc      780

auaagugaua uggcaucaga uucaagaugu ccuacucagg gagaagcaua uuuagauaaa      840

caaagugaua cacaauaugu auguaaaagg acauuaguag accguggaug gggaaaugga      900

uguggacuau uuggaaaagg aucauuaguu acaugcgcaa aauuugcaug uucuaagaaa      960

augacaggua aaagcauaca accagaaaau uuagaauauc gcauuaugcu aucaguucau     1020

ggaucccaac auucagguau gaucgugaac gauacagggc augaaacaga ugaaaauaga     1080

gcaaaaguag aaauaacacc aaacagccca agagcagaag caacuuuagg cggcuuugga     1140

aguuuaggau uagacuguga accaagaaca gguuuagauu uuucagaucu auacuauuua     1200

acaaugaaca auaaacauug guuaguccau aaagaauggu uucacgacau acccuuacca     1260

uggcacgcgg gugcagauac agguacucca cauuggaaca acaaagaagc auuaguagaa     1320

uuuaaggacg cucaugcaaa aaggcaaacu gugguaguau uaggaucaca agagggagcu     1380

guucacacag cauuagcagg agcucuagaa gcagagaugg auggagcaaa aggaagauua     1440

aguucgggac aucuaaaaug ucgacuaaaa auggacaagc uaagauuaaa aggaguaagu     1500

uauagucuau gcacagcagc uuuuacauuu accaaaauac cagcugaaac gcuacauggg     1560

acaguaacag uagaaguaca auaugcaggu acagaugggc cuuguaaagu accagcacaa     1620

auggcaguag auaugcaaac acuaacacca guaggacgau uaauaacagc aaauccagug     1680

auuacugaaa gcacagaaaa caguaaaaug augcuagaau uagacccacc cuuuggagac     1740

aguuauauag uaauaggcgu aggagaaaag aaaauaacuc aucacuggca uagaagugga     1800

agcacaauag gaaaagcauu ugaagcaaca guuagaggag caaaacgaau ggcugugcua     1860

ggagauacag cuugggauuu uggcucagua ggaggggcau uaaauucauu agguaaagga     1920

auacaucaaa uauucggugc agcauuuaaa agucuauuug guggaaugag uugguuuuca     1980

caaauauuaa uagguacucu acuaaugugg uugggauuaa acacgaaaaa cggaucaauc     2040

agucuaaugu gccuagcgcu ugggggcgug uuaauauuuu uaaguacagc aguaagugca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  4765
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4765
gggagaaagc uuaccaugaa aagacgugga gcugauacaa gugucggcau uguaggucug       60

uuauuaacaa cagcaauggc cgcagaagua acaagaagag gaucagcaua uuauauguau      120

uuagauagaa augaugccgg agaagcaaua ucuuuuccua caacauuagg gaugaauaaa      180

ugcuauauuc aaauaaugga uuuagggcac augugugaug caacgaugag uuacgaaugc      240

ccaauguuag augaaggagu agaaccggac gauguagauu guugguguaa cacuacauca      300

acauggguug uuuacggaac augucaucau aagaaaggag aagcaaggag aagucgcaga      360

gcagucacau uaccuaguca cucaacaaga aaauuacaaa caagaaguca aacaugguua      420

gaaaguagag aauauaccaa acaucuaaua agagucgaaa auuggauuuu uagaaacccg      480

ggauucgcac uggcagcagc ugccauugca uggcuccugg gaucaaguac gucacaaaaa      540

guaauauauu uaguaaugau cuuauuaauc gcaccggcau acucaauuag auguauagga      600

guaagcaaca gagauuuugu agaagggaug ucagguggaa cauggguaga uaucguauua      660

gaacauggcg ggugugucac aguaauggca caagauaaac cuacaguuga uauagaauua      720

gugacaacca caguuaguaa uauggcagaa guacgaucau auuguuauga ggcuuccaua      780

ucagacaugg caagugacuc acgaugccca acucaaggag aagccuauuu agacaaacaa      840

ucugauacac aauacguaug caaaaggacc uuagucgaca gagguugggg aaauggaugc      900

ggacuauuug ggaaagguag cuuaguaaca ugugcaaaau uugcaugcuc gaagaaaaug      960

acaggaaaaa gcauacaacc agaaaauuua gaauauagaa uaauguuaag cguucaugga     1020

ucacaacaca guggaaugau aguaaaugau acagggcaug aaacagacga aaauagagcu     1080

aaaguagaaa uaacuccaaa cagcccaaga gcggaagcaa cauuaggagg auuuggaagu     1140

uuaggacuag auugcgaacc cagaacagga cuggacuuua gcgaccuaua uuauuuaaca     1200

augaauaaua aacauuggcu gguucauaaa gaaugguuuc augauauucc uuuaccgugg     1260

caugccggug cagauacagg uacaccacau uggaauaaca aagaagcacu cguagaauuu     1320

aaagaugcac augcaaaaag acaaacagua guagucuuag gaucccaaga aggagcagua     1380

cacacugcuc uagcgggagc ccuagaagca gaaauggacg gcgcuaaagg aagauuaucu     1440

ucaggacauc uaaaauguag acuaaaaaug gauaagcuaa gauuaaaagg cguaucuuau     1500

agccuaugua cugcagcuuu uacauuuaca aaaauaccag cagaaacuuu acauggaaca     1560

guaacaguag aaguacaaua cgcaggaacu gacggaccau gcaaaguucc cgcacaaaug     1620

gcagucgaca ugcagacacu aacaccaguu ggaagauuaa uaacagcaaa uccaguaaua     1680

acagaaucaa cagaaaauuc uaaaaugaug cuugaauuag acccgcccuu cggagauuca     1740

uauauaguaa uugguguagg agaaaagaaa auaacccauc acuggcacag aaguggaucc     1800

acaauaggua aagcauuuga agcaacagua agaggugcca aaaggauggc aguccuagga     1860

gauacagccu gggauuuugg aaguguuggu ggggcacuua acaguuuagg aaaaggaaua     1920

caucaaauuu uuggagcagc auucaaaagu cuauucggag gaaugaguug guuuucacaa     1980

auacuaaucg gaacacuauu aaugugguua ggauuaaaua cuaaaaacgg aagcauauca     2040

uuaauguguc uugcauuagg cggagugcua aucuuuuuaa gcacagcagu aagcgcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  4766
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4766
gggagaaagc uuaccaugag aaggggugcg gauacaucug uaggaauagu aggauuauua       60

uuaacaacgg caauggcagc agaaguuaca agaagaggaa gugcuuacua cauguauuua      120

gauagaaacg augcugguga agccauaagu uucccaacua cauuaggaau gaacaaaugc      180

uacauccaaa ucauggaccu uggacauaug ugugaugcua caaugucaua cgaaugucca      240

augcuggaug aagguguaga acccgaugau guagacuguu gguguaacac aacaucuacu      300

uggguuguuu augguacgug ucaccauaag aaaggugaag caagacgauc aagacgagca      360

guaacauuac ccagucacuc aaccagaaaa cuacaaacaa gaucacaaac augguuagaa      420

ucaagagaau auacaaaaca ucuuauaaga guagaaaacu ggaucuuuag aaacccugga      480

uuugcauuag cagcagcagc uauagcaugg uuacuagguu cgaguacaag ucaaaaaguu      540

auuuaucucg uaaugauacu auuaauagca ccagcuuaua gcauaagaug uauaggugua      600

ucaaauagag auuuuguaga agggauguca ggagggacau ggguagauau cguauuagaa      660

cauggaggau guguaacagu cauggcgcaa gacaaaccaa caguugauau agaauuagua      720

acaacaacag uuuccaauau ggcagaaguu agaucauauu guuaugaagc uagcaucuca      780

gauauggcuu ccgacucaag auguccaaca caaggagaag cauauuuaga caaacaaucu      840

gauacacaau auguauguaa aagaacucua guagauagag guuggggaaa ugguugugga      900

uuauuuggca aaggaucuuu aguuacaugc gcaaaauuug cauguaguaa gaaaaugaca      960

ggaaaaagca uucaaccuga aaauuuagaa uauagaauaa ugcuaucagu acacggaagu     1020

caacauagcg guaugauagu aaacgauaca ggacaugaaa cagaugaaaa cagagcaaaa     1080

guggaaauaa caccaaacuc accuagagca gaagcaacau uaggcggauu ugguaguuua     1140

ggauuagacu gugaaccaag aacaggguua gauuuuagug auuuguauua uuuaacaaug     1200

aauaacaaac auuggcuagu acauaaagaa ugguuccacg auauaccauu accguggcau     1260

gcaggggcag auacuggaac uccccauugg aacaauaaag aagccuuggu agaauuuaaa     1320

gacgcacaug caaaacgaca aacagucgua guauuaggcu cacaagaagg cgcaguacau     1380

accgcuuuag caggcgcuuu agaagcagaa auggauggag ccaaaggaag gcuaucaucu     1440

ggucaucuua aauguagacu aaaaauggau aaacuaagac uaaaaggagu uucuuacagu     1500

cucuguacag cagcauuuac auucacgaaa auaccagcug aaacacuaca uggaacugua     1560

acaguagaag uacaauaugc aggaaccgau ggaccaugua aaguaccagc acaaauggca     1620

guugauaugc aaacauuaac cccaguaggg agacuaauaa ccgcaaaucc aguuauuaca     1680

gaaucaacag aaaacaguaa aaugaugcua gaauuagauc ccccuuuugg cgauucauac     1740

aucguaauag gugugggaga aaagaaaaua acgcaucacu ggcaucgauc aggaagcaca     1800

auaggaaaag cauuugaagc uacagucaga ggagcuaaaa gaauggccgu ucuaggagac     1860

acugcuuggg acuucggauc aguuggugga gcauuaaauu cucugggaaa aggaauacau     1920

caaauauuug gugcagccuu caaaucacua uuugguggca ugaguugguu cucacagaua     1980

cuaauaggua ccuuauuaau guggcuagga uuaaauacua aaaacggauc aauaucauug     2040

augugcuuag cccuaggagg aguuuuaaua uuucuaagca cagcugucag ugcaugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  4767
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4767
gggagaaagc uuaccaugcg aggagccgau acaagcguug gaauaguugg uuuauuauua       60

acaacagcua uggcagcaga aguaaccaga cguggaucgg cauauuauau guaccuagac      120

aggaaugaug caggagaagc aauaucuuuu cccacaacuu uaggaaugaa uaaaugcuau      180

auacaaauaa uggauuuagg acauaugugu gaugcaacua ugagcuauga augcccuaug      240

uuggaugaag ggguugaacc agaugaugua gauuguuggu gcaacacuac aucaaccugg      300

guuguauaug gaacauguca ucacaagaaa ggagaagcuc gcagaagcag acgagcagua      360

acacuaccuu cacauagcac aagaaaacua caaacacgau cucaaacaug guuagaaagc      420

agagaauaca caaaacaccu aauaagggua gaaaauugga uauuuagaaa cccaggguuu      480

gcauuagcag cagcggcuau agccuggcua uuaggaucua guacaucaca aaaaguaauc      540

uacuuaguga ugauauuauu aauugcacca gcauacucaa uaagauguau aggaguuagc      600

aauagagauu ucgucgaagg aaugucaggc ggaacauggg uagauauugu auuggaacau      660

ggaggaugcg uaacaguaau ggcacaagau aaaccuacug uagacauaga auuaguaaca      720

acaacuguaa gcaacauggc agaaguaagg ucauauuguu acgaagcaag uauaucagau      780

auggcaagug auucgagaug uccaacacaa ggcgaagcau auuuagacaa acaaucagau      840

acacaauaug uauguaaaag aacccuagua gauagaggau ggggaaaugg auguggucua      900

uuuggaaaag gaaguuuagu aacaugcgcu aaauucgcau gcagcaagaa aaugacagga      960

aaaucuauac aaccagaaaa uuuagaauac aggauaaugu uaucagucca cggaagucaa     1020

cauucaggaa ugauagucaa ugacacagga caugaaacug acgaaaauag agcaaaagua     1080

gaaauaacac caaacucacc aagagcagaa gcaacacuag guggcuucgg uucauuagga     1140

cuggacugug aaccaagaac aggauuagac uucagcgauu uauacuacuu aacuaugaau     1200

aacaaacauu ggcuaguaca uaaagaaugg uuccaugaca uaccauuacc uuggcaugcu     1260

ggugcagaua cuggaacacc acauuggaau aauaaagaag cgcuagugga guucaaagau     1320

gcacaugcua aacgacaaac aguaguagua uuaggcucac aagaaggagc cguccauaca     1380

gcacuugcag gagcacuuga agcagaaaug gauggagcaa aaggaagauu aucgaguggc     1440

cacuuaaaau gucgacuaaa aauggauaaa cuaagauuaa aaggaguuuc auauaguuua     1500

uguacagcag cauuuacauu caccaaaauu ccagcagaaa cauuacaugg aaccguaacu     1560

guggaaguac aauaugcagg aacagaugga cccuguaaag uaccagcaca aauggcaguc     1620

gauaugcaaa ccuuaacacc aguaggaaga uuaaucaccg caaacccugu gauaacagaa     1680

ucuacagaaa acaguaaaau gaugcuagaa uuggauccuc cauucggaga uuccuauaua     1740

guaauaggag uaggagaaaa gaaaauuacu caccacuggc acagaucagg uagcaccaua     1800

gguaaagcau uugaagcuac aguaagaggu gcaaaaagga uggcuguauu aggcgacaca     1860

gcaugggauu ucggaucagu uggaggcgcu uuaaacucac ucggaaaagg cauucaucaa     1920

auauuuggag cagcauuuaa aaguuuauuu ggaggaaugu caugguuuuc acaaauucua     1980

auaggaacau uacuaaugug gcugggucuc aauaccaaaa acggcaguau cagcuugaug     2040

ugucuagcau uaggaggcgu acuaauauuc cuaagcacag caguaucagc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4768
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4768
gggagaaagc uuaccauggg cgcagauaca aguguaggaa uuguaggguu auuacuaaca       60

acagcaaugg cagcagaagu aacaagaaga gguagugcau auuauaugua ucuugauaga      120

aacgaugcag gagaagcuau uucauuucca acaacacuag gcaugaauaa auguuauaua      180

caaaucaugg auuuaggaca caugugcgau gcuacaauga guuaugaaug uccaaugcua      240

gacgaaggcg uugaaccaga ugauguagau ugcuggugua acacaacaag uacuugggua      300

guuuauggca ccugucauca caagaaagga gaagcgcgaa gaaguagacg agcaguaaca      360

uuaccaucac auagcacaag aaaauugcag acuagaaguc aaacauggcu ugaaucaaga      420

gaauauacaa aacauuuaau acgaguugaa aacuggauau uccgaaaucc agguuuugca      480

cuagcagcag cagcaauagc auggcuacug ggaucaagua cuagccaaaa aguaauauau      540

uuaguaauga uauuacuaau agcccccgcg uacaguauaa ggugcauagg uguaucgaau      600

agggauuuug uugaaggaau gagcggaggg acuugggugg auauaguacu agaacaugga      660

gguuguguua caguaauggc acaagauaaa ccaacaguug acauagaauu agugacaaca      720

acuguaagca auauggcuga aguaaggagc uauugcuaug aagcaaguau aucagauaug      780

gcaagugauu caagaugucc aacacaaggu gaagcauacu uagauaaaca auccgauacu      840

caauauguau guaaaagaac ucuaguagau cgaggauggg gaaacggaug uggauuauuu      900

ggaaaaggga guuuaguaac augcgcaaaa uuugcaugua guaagaaaau gacaggaaaa      960

ucaauacagc cagaaaaucu agaauaucgg auaaugcuau caguacaugg uagucaacau     1020

ucaggaauga uaguaaacga uacaggucac gaaacagacg aaaaucgagc aaaaguugaa     1080

auaacaccua acaguccacg agcugaagca acauuaggcg gauuuggauc ccuaggauua     1140

gacugugaac cgaggacagg acuagauuuu ucagaucuau auuauuuaac aaugaacaau     1200

aaacauuggc uaguucauaa agaaugguuu caugauauac cauuaccaug gcaugcuggu     1260

gcagacacag gaacaccuca uuggaauaau aaagaagcau uaguagaauu uaaagacgca     1320

cacgcaaaaa gacaaacagu uguaguauua ggaagccaag aaggagcugu acauacagca     1380

cuugcuggag cacuagaagc ugaaauggau ggugcuaaag gcagauuaag caguggucau     1440

uuaaaaugca gauugaaaau ggauaaauua cgauuaaaag gaguaucaua uuccuuaugc     1500

acagcagcau uuacauuuac aaaaauacca gcagaaacau uacauggaac aguaacagua     1560

gaaguccaau augcaggaac agacggacca ugcaaaguac cagcacaaau ggcaguugau     1620

augcagacac uaacaccagu aggaagauua auaacugcaa acccgguaau aaccgaaagu     1680

acagaaaauu caaaaaugau guuggagcug gauccaccau uuggggacuc auacauagua     1740

auuggcguag gagaaaagaa aauuacacac cacuggcauc gaaguggaag cacaauagga     1800

aaagcauucg aagccacagu aaggggagca aaaagaaugg caguauuagg ugacacagca     1860

ugggacuucg guucaguagg aggugcuuua aauaguuuag guaaaggaau acaccaaaua     1920

uuuggagcag cauuuaaauc auuauuugga ggaaugaguu gguuuucaca aauucugaua     1980

ggaacguuac uaauguggcu uggcuuaaau acgaaaaaug gaagcauuuc auuaaugugu     2040

uuagcgcuug guggaguuuu aauauuuuua ucuacugcag uuagcgcgug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4769
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4769
gggagaaagc uuaccauggc agauacgucu gucggaauag uuggauuacu acuaacaacu       60

gcaauggccg cagaagugac uagacgaggu ucggcguacu acauguaucu agacagaaau      120

gaugcuggag aagccauauc auuuccaacu acauuaggaa ugaauaaaug cuacauacaa      180

auuauggacc uaggacauau gugugacgca acaaugucau augaaugucc aauguuagau      240

gaagggguag aaccagacga cguagauugc ugguguaaca caacaucuac augggugguu      300

uacgguacau gucaucauaa gaaaggugaa gcaagacgcu caagacgagc aguaacacug      360

ccaucacaua guacaagaaa acuacaaaca agaucacaaa cauggcucga aucgagagaa      420

uacacaaaac aucuuauaag aguagaaaau uggauauuca gaaaucccgg cuucgcacua      480

gcugcagcag caauagcaug guuacuugga aguucuacga gucaaaaagu aauauaucua      540

guaaugauac uucuaaucgc gccugcauau agcauaaggu guaucggggu cucaaauaga      600

gacuuuguag aaggaauguc aggaggaaca uggguagaua uuguguuaga acauggugga      660

ugugucacag uaauggcaca agacaaaccc acaguagaca uagaauuagu uacuaccaca      720

guaucaaaca uggcagaagu ucgaucguau uguuaugaag cauccauaag ugauauggca      780

ucagacagua gaugcccuac ccaaggagaa gcauauuuag auaaacaaag cgauacucaa      840

uacguaugua aaagaacccu aguagaucgu ggauggggua acggaugugg uuuauuuggu      900

aaaggcaguu uaguaacuug ugcaaaguuu gcauguucaa agaaaaugac agguaaaucu      960

auacaaccgg aaaaccuaga auaucgaaua augcuaagug uacauggauc ucagcauagu     1020

ggaaugauag uaaaugacac agggcaugaa acagaugaaa acagagcgaa aguagaaaua     1080

acuccaaaca gcccaagagc agaagcaaca uuaggaggau uuggcagucu aggacuagau     1140

ugugaaccaa gaacuggauu agauuuuuca gaucuuuacu acuuaacuau gaacaacaaa     1200

cauugguuag uacacaaaga augguuucau gacauaccgc uaccauggca cgcuggugca     1260

gauaccggca caccucauug gaauaauaaa gaagccuuag uagaauuuaa agacgcacau     1320

gcaaaaagac aaacaguagu aguacuagga ucacaggaag gagcaguaca uacagcccua     1380

gcuggagcau uagaagcgga aauggacgga gcuaaaggaa gauugucaag cggacacuua     1440

aaaugucgac uaaaaaugga uaaacuaaga cuuaaaggag uaucauauag cuuaugcaca     1500

gcagcauuua cauuuacaaa aauaccggca gaaaccuugc augggacugu aacaguagaa     1560

guacaauaug cagggacaga uggcccaugu aaaguaccag cacaaauggc aguagauaug     1620

caaacccuaa cuccaguagg aagauuaaua accgcaaauc caguaauaac ugaaagcacg     1680

gaaaacucaa aaaugaugcu agaacuagau ccaccauucg gagacaguua uauaguaaua     1740

ggcguaggag aaaagaaaau aacgcaccau uggcauagaa gcggaaguac aaucggaaaa     1800

gcauuugaag caacaguaag aggcgcaaaa agaauggcag uacuaggaga uacagcaugg     1860

gacuuuggau cgguaggagg ggcauuaaac aguuuaggga aaggaauaca ucaaauauuu     1920

ggagcagcau uuaaaucacu auucggagga augagcuggu ucucacaaau auuaauaggg     1980

acauuauuga uguggcuagg ccuuaacacu aaaaauggau caauaagucu aauguguuug     2040

gcauuaggcg gaguacuaau auuuuuauca acagcaguau cagcaugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  4770
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4770
gggagaaagc uuaccaugga uaccucaguu ggaauaguag gacugcuauu aacaacagcu       60

auggcugccg aaguaacaag aagaggaagc gcauacuaua uguacuuaga uagaaaugau      120

gcgggagaag caauaaguuu uccaaccaca cuaggaauga auaaauguua cauacaaaua      180

auggaucuag ggcauaugug ugaugcgaca augucauaug aauguccaau guuggaugaa      240

ggcguagaac ccgaugaugu agacuguugg uguaacacaa caagcacaug gguaguauau      300

ggaacauguc aucacaagaa aggagaagca cgaagaagua ggagagcagu uacauuacca      360

ucacauagua cuagaaaauu gcaaacuaga ucacaaacau gguuagaaag ccgcgaauau      420

acaaaacauu ugauaagagu agaaaauugg auuuuuagaa acccaggcuu cgcacuagcc      480

gcagcagcaa ucgcauggcu ucuaggaagc ucuaccucac aaaaaguaau auauuuggua      540

augauauuau uaauagcacc agcuuauagu auaagaugua ucggaguaag uaauagagau      600

uuuguagaag gaaugagugg aggaacaugg guagacauag ucuuagaaca uggaggaugu      660

guuacaguaa uggcgcaaga uaaaccaaca guagauauag aauuaguaac cacaacagua      720

aguaauaugg cagaaguaag aaguuauugu uaugaagcaa gcauuaguga uauggcauca      780

gauagucgau guccaacaca gggagaagca uacuuggaca aacaaaguga cacucaguau      840

guauguaaga gaacauuggu agauagagga ugggguaaug guuguggauu auucggaaaa      900

ggaucguugg ucaccugugc aaaauuugcu uguagcaaga aaaugaccgg aaaaucaauu      960

cagccagaaa acuuagaaua uagaauaaug uuaagcguuc auggaaguca acauagcggc     1020

augauaguaa augauacagg acacgaaaca gacgaaaaca gagcaaaagu agaaauuaca     1080

ccaaauaguc caagagcaga agcaacauua ggagguuuug gaucucuugg auuggauugu     1140

gaaccaagga ccggacugga uuuuucagac cuauauuacu uaacaaugaa uaauaaacau     1200

ugguuaguac acaaagaaug guuucacgac auuccauuac cuuggcacgc cggagcagau     1260

acugggacac cgcauuggaa uaauaaagaa gcauuaguag aauuuaaaga cgcacacgca     1320

aaacgacaaa caguaguugu ucuaggaagu caagaggggg caguucauac agccuuagca     1380

ggggcacuag aggcagaaau ggauggagcu aaaggaagac uaaguagugg acacuuaaaa     1440

uguagauuaa aaauggacaa auuacgucuc aagggagucu ccuauagcuu guguacagca     1500

gcauuuacuu ucacaaaaau accagcagaa acuuuacaug gaacaguaac cguagaagua     1560

caauaugcag gaacagaugg accaugcaaa guaccagcac aaauggcagu cgacaugcaa     1620

acacuaacac caguagggag acuaaucaca gcaaauccug uaauaacaga aucaacugaa     1680

aauagcaaga ugauguuaga auuagaucca ccauuuggag auucauacau aguaauugga     1740

guaggugaaa agaagauaac acaucacugg cacagaagug gaucaaccau aggaaaggcu     1800

uuugaagcaa ccguaagagg ugcaaaacga auggcuguuu uaggagacac agcaugggac     1860

uuugggucag uagguggagc auuaaauagu cuaggaaaag guauucauca aauauuugga     1920

gcagcuuuua aaaguuuauu cggaggaaug ucaugguuca gccaaauauu gauaggaacu     1980

uuauuaaugu ggcuaggauu gaauaccaaa aaugguagua uuagucuaau gugucuugcg     2040

uuaggaggag uauuaauauu uuuaagcaca gcaguaucag caugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  4771
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  4771
gggagaaagc uuaccaugac aagcguagga auaguuggau uauuacuaac aacagcaaug       60

gcagcugaag uaacuagaag aggaagcgca uauuauaugu accuagaucg aaacgacgcu      120

ggagaagcaa uaucuuuccc aacuacauua ggcaugaaua aaugcuauau acaaauaaug      180

gauuuaggac auaugugcga ugcaacaaug ucauacgaau gucccauguu agacgaagga      240

guagaaccag augauguaga uuguuggugc aauacaacau caacaugggu cgucuauggc      300

acaugucauc auaagaaagg ugaagcacgg agaagucgca gagcaguuac acuaccguca      360

cauucaacaa gaaaacuaca aacuagauca caaaccuggu uggaaaguag agaauauaca      420

aaacaucuaa uaaggguaga aaauuggaua uucagaaacc ccggauuugc uuuagcugca      480

gcagcaauag cauggcuacu aggaaguagc acaucacaaa aaguaauaua ucuaguaaug      540

auacuacuaa uagcgccagc guauaguaua agauguauag gaguaucuaa ccgugauuuu      600

guggaaggga ugucaggugg aacaugggua gauauuguau uagaacaugg aggaugugua      660

acagucaugg cgcaagacaa accaacugua gauauagagc uaguaaccac aacuguauca      720

aauauggcag aaguuagauc cuauugcuac gaagcaucga uuagcgauau ggcaagugau      780

uccagauguc caacccaagg agaggcuuac uuagauaaac aaagugauac acaauaugua      840

ugcaaaagaa cauuagucga uagaggaugg ggaaauggau gcggguuauu cggaaaagga      900

ucuuuaguga caugugcaaa auuugcuugu ucuaagaaaa ugacaggcaa aucaauacaa      960

ccagaaaacu uagaauauag aauuauguua ucaguacacg gaucacaaca uagcggaaug     1020

auugucaaug auacaggaca ugaaacagau gaaaauagag caaaaguaga aauaacaccu     1080

aauucaccua gagcagaagc aacacuagga ggauucggaa guuuagguuu ggauugcgaa     1140

ccacgaacug gucuagauuu uucagauuua uacuauuuga caaugaauaa uaaacauugg     1200

uugguacaua aagaaugguu ucaugacaua ccacuaccuu ggcaugcagg agcagacacu     1260

ggaacaccac acuggaacaa uaaagaagca cuugucgagu ucaaagaugc acaugcuaaa     1320

agacaaacag uuguuguauu aggaagccaa gaaggagcag uacauacagc auuagcaggg     1380

gcguuagaag cagaaaugga uggagcaaaa ggcagacuuu cuuccggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu acgccuaaaa ggugucucau acucauuaug uacagcagca     1500

uuuaccuuua ccaaaauucc agcggaaacc cuucauggaa ccguaacagu ugaaguucaa     1560

uaugcuggca cagacggacc augcaaagua ccugcacaaa uggcaguaga uaugcaaacu     1620

cuaacuccag uugggagacu aauaacagcc aauccaguua ucacagaaag cacagaaaau     1680

ucaaagauga ugcucgaauu agauccgcca uucggugacu cuuauauagu uauaggagua     1740

ggugaaaaga aaauaacaca ucauuggcac cguagcggaa gcacuauugg aaaagcauuu     1800

gaagcaacag uuagaggugc aaaaagaaug gcaguacuag gagauaccgc augggauuuc     1860

ggaagugucg gcggagcacu aaauucauua gguaaaggua uacaucaaau auuuggagca     1920

gcuuuuaaaa gccuauuugg uggaaugagu ugguucucgc aaauauuaau aggaacccuu     1980

uuaauguggc uaggacuaaa uacuaaaaau ggaaguauaa gccucaugug ucuagcacuu     2040

gggggaguau uaauauucuu auccacagcu guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4772
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4772
gggagaaagc uuaccaugac gucaguagga auugucggau uauuauuaac uacagcaaug       60

gcagcagcug uaaccagaag gggaucagcc uacuauaugu auuuggaccg aaaugaugcu      120

ggugaagcaa uaucauuucc aacuacauua gguaugaaua aaugcuauau acaaauaaug      180

gaucuuggac auauguguga ugcaaccaug aguuaugaau guccuauguu agaugaagga      240

guagaaccug augacguaga uugcuggugu aacacaacuu caacaugggu aguauaugga      300

acaugccauc acaagaaagg agaagcaaga cgaagccgga gagcaguaac auuaccaagu      360

cacucaacga gaaaauuaca aacaagauca caaacauggc uugaaaguag agaauacacu      420

aaacauuuaa uaagagucga aaacuggaua uuuagaaauc caggauuugc acuagcagcu      480

gcagccauug caugguuauu aggaucaagu acaucucaaa aagucauuua cuuaguaaug      540

auauuacuca uagcuccugc auauucaauc agaugcauag gcguuucaaa cagagauuuc      600

guagaaggaa ugucaggugg aacaugggua gauaucguac uagaacacgg aggaugcguu      660

acaguaaugg cacaggauaa accaaccgua gacauagaau uaguaacaac uacuguaagu      720

aauauggccg aaguaagauc auauuguuau gaagccagua uuagcgauau ggccagcgau      780

agcagauguc caacgcaagg agaagcauac uuagauaaac aaagugauac ucaauaugua      840

uguaaacgaa cauuaguaga ucgaggaugg gguaacggau gcgguuuauu uggaaaagga      900

ucauuaguaa caugcgcaaa auuugcaugc ucuaagaaaa ugacuggaaa aucaauacaa      960

ccagaaaauc uagaauacag aauaauguua ucgguacaug gaagccaaca uucaggaaug     1020

auaguaaacg acacuggaca ugaaaccgac gaaaauagag ccaaaguaga aauaacaccu     1080

aauuccccaa gagcagaagc aacauuagga ggauucggaa gccuaggacu agauugugaa     1140

ccaagaacug gucuugacuu cucagacuua uacuaccuaa ccaugaacaa caaacacugg     1200

uuaguacaua aagaaugguu ccacgacaua ccauuaccuu ggcacgcugg agcagacaca     1260

ggcacacccc acuggaauaa uaaagaggcu uuaguugagu ucaaagaugc ucacgcuaaa     1320

agacaaacag uaguaguacu agguucacaa gaaggugcag uacauacagc uuuagcagga     1380

gcacucgaag cagaaaugga uggagcaaaa ggaagauuau caaguggaca ucuaaagugc     1440

aggcuaaaaa uggauaaguu aaggcuaaaa ggaguaucau auucucuaug cacagcagca     1500

uuuacauuca caaaaauacc agcagaaacc uuacauggca ccguaacagu agaaguacaa     1560

uaugcaggua cagauggccc uuguaaaguc ccagcacaaa uggccguaga uaugcaaaca     1620

uuaacaccag ugggcagauu aauaacagca aaccccguaa uaacugaaag uacagaaaau     1680

aguaaaauga ugcuagaauu agauccucca uuuggcgaua guuauauagu aauaggagua     1740

ggagaaaaga aaauaacaca ucacuggcac cgcagcggaa guacuauugg aaaagcauuu     1800

gaagcaacug uaagaggagc aaaaagaaug gcuguucuag gagacacagc uugggacuuu     1860

gguucuguag gaggagcacu aaauaguuug ggaaaaggca uacaucaaau uuuuggcgcu     1920

gcauucaaau cacuauuugg aggaaugagu ugguuuucac aaauucucau aggaacauua     1980

uuaauguggc uaggguuaaa cacgaaaaau ggaaguauau cauuaaugug uuuagccuua     2040

gguggaguau uaaucuucuu aucuacagca guaucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4773
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4773
gggagaaagc uuaccaugcg uggagcugau accucaguug guauaguagg cuuacuauua       60

acaacagcaa uggcugccgc uguuacuaga agaggaucag cauauuauau guacuuagau      120

agaaaugaug caggagaagc aaucucauuu cccacuacuu uaggcaugaa uaaauguuac      180

auucaaauaa uggauuuagg acauaugugu gaugcaacaa ugucauacga augcccaaug      240

uuagaugaag gcguagagcc cgaugaugua gauuguuggu guaauacuac aucuacuugg      300

guaguguaug gaacauguca ucacaaaaaa ggagaagcaa gacgaagcag acgagcagua      360

acauugccau cucacucaac aagaaaauua caaacaagau cacaaacaug gcuagaaucu      420

agagaauaca caaaacauuu aauaagagua gaaaauugga uauuuagaaa uccaggauuc      480

gcguuggcgg ccgcagcgau agcaugguua uuaggaucaa guacaucaca aaaaguuauc      540

uauuuaguaa ugauccuacu aauagcacca gcguauagua uuagauguau aggcguauca      600

aauagagauu ucguagaggg gaugaguggu ggaacauggg uagauauagu uuuagaacau      660

ggugggugug uaacgguaau ggcucaagau aaaccaaccg uagauauaga guuagucaca      720

acuacaguca gcaauauggc cgaaguaaga ucauacuguu augaagcauc aaucucagau      780

auggccagug acaguagaug uccaacucaa ggagaagcuu aucucgacaa acaaagugau      840

acacaauaug uauguaaaag gacacuagug gauagagguu gggguaaugg uuguggacuc      900

uuuggcaaag guagucuagu cacgugcgcu aaauuugcuu gcucaaaaaa aaugacggga      960

aaaagcauac aaccugaaaa uuuagaauac cgaauaaugu uaucaguuca uggcagucaa     1020

cauagcggga ugauaguuaa cgauacagga caugaaacag augaaaauag agcaaaagug     1080

gaaauaacac cuaacucacc uagagcggaa gccacguuag gaggauuugg aaguuuagga     1140

uuagauugcg aaccaaggac aggauuagau uucucagauu uauauuaucu aacuaugaau     1200

aacaaacacu gguuaguaca uaaagagugg uuccacgaua uuccacuacc auggcaugcu     1260

ggggcagaca cuggaacacc ucauuggaau aauaaagaag cauuaguaga auucaaagac     1320

gcacaugcua aaagacaaac uguaguggua cuaggaaguc aagaaggagc aguacauacc     1380

gcacuugcug gagcacuaga agcagaaaug gauggugcaa aaggaagacu uucaagugga     1440

caucuaaaau guagauuaaa aauggacaaa uuaagauuaa aaggaguauc auacucauua     1500

uguacagcag ccuuuacauu cacaaaaaua ccugcagaaa cacuucaugg aacaguaaca     1560

guagaaguac aauaugcugg aacugauggc ccguguaaag uucccgcuca aauggcaguu     1620

gauaugcaaa cauuaacccc ugucgggaga uugauaacag cuaauccagu uauaacagaa     1680

ucuacugaaa acucaaaaau gauguuagaa uuagacccac cauucggaga uagcuauaua     1740

guaaucgggg uaggugaaaa aaaaauuaca caccauuggc acagaagugg aucuacaaua     1800

ggaaaagcau uugaagccac uguuagagga gcaaaacgaa uggcaguacu aggugacacc     1860

gcaugggauu uuggaucagu cggcggugca uuaaacucac uaggaaaagg aauccaucaa     1920

auauuuggag cugcauuuaa aagccuauuu gguggaaugu caugguuuag ccaaauacua     1980

auuggaacuu uauuaaugug gcuaggauua aauacaaaaa auggaucaau uagcuuaaug     2040

uguuuagccu uaggaggagu uuuaauauuc uuaagcacag cuguaagugc augaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  4774
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4774
gggagaaagc uuaccaugag aggacuaucu agcagaaaaa gaagaucaca ugacguauua       60

accguucagu ucuuaauauu aggaauguua uuaaugacag gaggaguaac aagacguggc      120

uccgcguauu auauguaccu agauagaaau gaugcaggag aagcaauuuc auuuccuaca      180

acuuuaggaa ugaauaaaug cuauauacaa aucauggacc uaggacauau gugcgaugcc      240

acaaugagcu augaaugucc aauguuagau gaaggcguag aaccagauga cgucgauugc      300

ugguguaaca caacuucaac cuggguagua uauggaaccu gucaucauaa gaaaggagaa      360

gcaagacgau caaggcgagc aguaacauua ccaucucacu caacaagaaa auuacagaca      420

agaucacaaa cauggcuaga aaguagagaa uauacaaaac auuuaauaag agucgaaaau      480

uggaucuuuc guaauccugg uuuugcacua gcugcagcug cuauagcuug guuauuaggc      540

ucaagcacau cacaaaaagu uauuuacuua guaaugauau uacuaauagc accagcuuac      600

ucaauaagau guauaggugu uuccaacaga gauuuuguag aagguauguc uggaggaacu      660

uggguugaua ucgucuugga gcauggugga uguguaacgg uaauggcuca agauaaaccu      720

acaguagaua uagagcuagu uacgacaaca guuagcaaua uggcugaagu ccgaaguuau      780

ugcuacgaag caagcauaag ugauauggca agugauucaa gaugcccaac acaaggagaa      840

gcauaucuag auaaacaaag ugacacacaa uacguuugua aaagaacucu uguagauagg      900

gguuggggca auggaugugg uuuauucgga aaaggaaguu uaguaacaug ugcuaaauuu      960

gcuuguucua agaaaaugac uggcaaauca auucaaccag aaaauuuaga auacagaaua     1020

augcuaucag uucaugguuc acaacauucu ggaaugauag uaaaugauac aggacaugaa     1080

acagaugaaa aucgugcuaa aguagaaauu acaccaaacu caccaagagc agaagcaacu     1140

cuugguggau uuggcucauu gggguuggau ugcgaaccaa gaacuggauu agauuucagu     1200

gauuuguauu auuuaacuau gaauaauaaa cacuggcuag uucauaaaga gugguuccac     1260

gacauaccau uaccauggca cgcaggagca gauacaggaa caccacauug gaacaauaaa     1320

gaagcacucg uugaauuuaa agaugcacau gcaaaaagac aaaccguggu aguauuggga     1380

ucucaagaag gagcaguuca cacagcacua gcaggagcau uagaagccga aauggacgga     1440

gcaaaaggaa gguuaucuuc agggcaucua aaauguagau uaaaaaugga caaacuaaga     1500

uuaaaagggg uaaguuauuc acuauguaca gcagcuuuua cauucacaaa aauaccugca     1560

gaaacacugc acgggacagu aacaguagaa guacaauaug caggaacaga ugguccaugu     1620

aagguacccg cacaaauggc aguugacaug caaacucuaa cgccaguagg aagacuaauc     1680

acagcuaauc caguaauaac agaaucaacu gaaaacucaa aaaugaugcu agagcucgau     1740

ccaccuuucg gagauucaua cauugugaua gguguuggag aaaagaaaau uacacaucau     1800

uggcauagau caggaucaac uauaggaaaa gcauuugaag ccaccguaag aggagcaaaa     1860

agaauggcug ugcuaggaga uacugccugg gauuuuggaa guguaggagg agcauuaaau     1920

ucacuaggga aaggaauaca ucaaauauuu ggagcagcau uuaaaucacu auuugguggu     1980

augagcuggu uuucacaaau ucuaauagga acauuauuaa ugugguuagg ucuaaacacu     2040

aaaaacggaa gcauaucuuu aaugugccua gcacuaggug ggguacucau auuucuaagc     2100

acugcuguga gugcaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4775
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4775
gggagaaagc uuaccaugag aggguuauca agucgaaaaa ggagaucaca ugauguuuua       60

acaguacaau uuuuaauuuu gggcaugcua cuuaugacug gagcaguaac aaggagagga      120

agugccuauu auauguaucu ugauagaaau gaugcaggag aagcaauuuc auuuccaaca      180

acauuaggaa ugaauaaaug uuauauacaa auaauggauu uaggacacau gugugaugca      240

acaaugucau acgaaugucc aaugcuagau gaaggugugg aaccugauga uguagauugc      300

ugguguaaca caacaagcac uuggguagua uacggaacau gucaccacaa gaaaggggaa      360

gcaagaagaa guagaagggc aguuacccua ccaagccauu caacaagaaa auuacaaaca      420

agaucacaaa cauggcuaga aaguagagaa uacacaaaac acuuaauaag aguagaaaac      480

uggaucuuua gaaauccagg auucgcacua gcagcugcug cuauugcuug gcuauuagga      540

agcaguacua gccaaaaggu aauauaucuu guuaugauau uacuaauagc accagcauac      600

ucaauaaggu gcauaggagu aucaaauaga gauuuuguag aaggaauguc cggagguaca      660

uggguggaua uuguacuaga acauggcggc ugcgucacag ugauggcaca agacaaacca      720

acaguagaua uagaauuagu uacaacaaca gucucgaaua uggcagaagu aagaaguuau      780

uguuacgaag caagcauaag ugauauggca agcgacaguc ggugcccuac ucagggagaa      840

gcauauuuag auaaacaauc agauacccaa uacguaugua aacgaacucu aguagacaga      900

ggguggggaa auggaugugg acuauuuggu aaaggaucac ugguaacaug cgcaaaauuu      960

gcaugcagua agaaaaugac aggcaaaagu auucaaccug aaaauuugga auauagaaua     1020

augcuuagcg uacauggauc acaacacuca ggaaugauag ugaaugauac aggacacgaa     1080

acagaugaaa auagagcaaa aguagaaaua acaccuaaua guccuagagc ggaagcaaca     1140

cuaggaggau ucggaucauu gggucuugac ugugaaccac gaacuggguu agauuuuuca     1200

gaucuauauu auuuaacaau gaauaauaaa cauugguuag uacacaaaga gugguuccau     1260

gauauaccau ugccauggca ugcaggagcg gauacuggaa caccgcauug gaacaacaaa     1320

gaagcacuug uagaauuuaa agaugcacau gcaaaaaggc aaacaguagu aguacuugga     1380

ucucaagaag gagcaguaca uacugcuuua gcaggagcau uagaagcuga aauggaugga     1440

gcaaaaggua gacuaagcuc gggacacuua aaaugcagac uaaagaugga uaaauuaaga     1500

uuaaaaggug ugucguacag uuuauguaca gcugcauuua ccuuuacuaa aauaccagca     1560

gaaacauuac auggcacugu aacaguugaa guacaauaug caggaaccga ugguccaugc     1620

aaaguaccag cucaaauggc aguagauaug caaacgcuua cuccaguagg aagguuaaua     1680

acagcuaauc caguaauaac ugaauccacc gaaaauagca aaaugauguu agaauuagau     1740

ccaccauuug gggauucaua uauaguuaua ggaguaggag aaaagaaaau aacucaccau     1800

uggcacagau caggaaguac aauaggaaaa gcguucgaag caacaguaag gggagcaaaa     1860

cgaauggcag uauuaggaga cacagcaugg gacuuugguu caguaggcgg agcauugaau     1920

agcuuaggaa aaggaauaca ccagauauuu ggcgcagcuu ucaagagcuu auuuggagga     1980

augucguggu uuagccaaau acuaauagga acuuuauuga uguggcuagg acugaacacu     2040

aaaaacggaa guauuucauu aauguguuua gcauuaggag gaguauuaau cuuuuuauca     2100

acagcaguaa gugcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4776
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 1 opt

<400>  4776
gggagaaagc uuaccaugcg uauaauaaau gcaagaaaag aaaagaaaag aagaggcgca       60

gauacaagcg uaggcauagu uggguuacug uuaacaacag cuauggcugc agaaguaaca      120

agaagaggua gcgcuuauua cauguauuua gacaggaaug acgcuggaga agcaauuagc      180

uuuccaacaa cuuuaggaau gaauaaaugu uauauucaaa uaauggaucu aggacacaug      240

ugcgacgcaa ccaugaguua ugaaugucca augcuagacg aaggaguaga acccgaugau      300

guugauuguu gguguaauac aacgucaacc uggguuguau auggcacaug ucaucauaag      360

aaaggagaag caagaagaag uaggagagca guaacacuac cuucacauuc aacuagaaaa      420

cuacaaacuc guagccaaac auggcuagaa ucacgagaau auacaaaaca ucugauaaga      480

guagaaaauu ggauuuuuag aaauccugga uuugcucuag cugcagccgc aauagcaugg      540

cuacuaggau cuaguacuag ccaaaaagug auauacuuag uaaugauauu acuaauagcu      600

ccugccuaca guauacgcug cauaggagua agcaauaggg acuucguaga gggaauguca      660

ggaggcacau ggguagauau aguacuagaa cacggaggau guguaacagu aauggcccag      720

gauaaaccaa cuguagauau ugagcuagua acuacaacug uaucaaacau ggcugaaguc      780

agaaguuacu guuacgaagc aaguauaagu gauauggccu cagauucacg auguccaacu      840

caaggagaag ccuaucuaga uaaacaauca gauacucaau auguauguaa aagaacuuua      900

guagauagag gcuggggaaa uggcugugga uuauuuggaa aaggcagcuu agucacaugc      960

gcaaaauuug ccugcagcaa gaaaaugacg ggaaaaucga uacaaccgga aaauuuagaa     1020

uauagaauaa uguuaucagu acacgggagc caacauucag gaaugauagu aaaugauaca     1080

ggacacgaaa cggaugaaaa uagagccaaa guugaaauaa caccuaacag uccaagagca     1140

gaagcuacac uagguggauu cggcucauua ggauuggauu gugaaccacg aacuggcuua     1200

gacuucucug acuuauacua cuuaacuaug aauaauaaac auuggcuagu acauaaagaa     1260

ugguuccaug acaucccuuu accauggcau gcaggagcag auacaggaac accucauugg     1320

aauaauaaag aagcacuugu agaguucaaa gaugcacaug cuaaaagaca aacaguagua     1380

guauuaggau cacaagaagg ugccguacac acugcgcuag cuggugcauu agaagcggaa     1440

auggauggag ccaaaggaag acuaucuucc ggacaucuua aauguagauu aaaaauggau     1500

aaacuaagau uaaaaggagu aagcuauagu uuaugcacag cagcuuuuac auuuacaaaa     1560

auaccggcag aaacauuaca uggaacagua acaguugaag uucaauaugc gggaacggau     1620

ggaccuugca aagucccagc ccaaauggcu guggacaugc aaacauuaac gccaguagga     1680

agacucauua ccgcaaaucc aguaauaacu gaaucaacgg aaaacaguaa aaugaugcua     1740

gaacuagacc cuccauuugg agacagcuau auaguaauag gaguaggaga aaagaaaaua     1800

acacaucauu ggcaucgauc uggaaguacc aucggaaaag cauuugaagc gacaguacga     1860

ggagccaaaa gaauggccgu auuaggcgau acagcuuggg auuucgguuc ggucggaggc     1920

gcauuaaaua guuuaggaaa aggaauacau caaauuuucg gagcagcauu caaaagccua     1980

uuugggggca ugagcugguu uucacaaaua cucaucggua cuuuauuaau guggcuagga     2040

cucaauacga aaaaugguag uaucucauua auguguuuag cauuaggcgg aguauugauu     2100

uuucuuagua cagcaguaag ugccgacgua ggaugcucag uagauuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4777
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 1 opt

<400>  4777
gggagaaagc uuaccaugag aauuauaaac gcuagaaaag agaaaaagcg uagaggagcu       60

gauacaagcg uugggauagu agguuuguua cuaacaacag caauggcagc agaaguuaca      120

agaagaggga gugcauauua cauguaucua gacaggaacg augcugguga agcaauuuca      180

uucccaacaa cauuaggaau gaauaaaugu uauauacaaa uaauggacuu aggacacaca      240

ugcgaugcaa caaugucaua ugaguguccg auguuagaug agggaguaga accagaugac      300

guagauuguu gguguaauac aacaaguaca ugggucgugu auggaacaug ccaccauaag      360

aaaggagaag caagaagaag uagacgagca guaacauuac caagucacag uacaagaaaa      420

cuacaaacua gaagucaaac augguuagaa ucuagagagu auacaaaaca cuuaaucaga      480

guagaaaauu ggauauuuag aaauccagga uuugcauuag cagcugcggc aauagcuugg      540

cuauuaggaa gcucaacauc ucaaaaagua auauauuuag uaaugauacu auuaauagca      600

ccagcuuaua gcauaaggug uauuggagua ucaaauagag auuucguuga aggaauguca      660

ggaggaacau ggguagaugu uguucuagaa cauggagguu gcguuacagu cauggcacaa      720

gauaaaccaa caguagauau agaauuaguc acaacaacag uuaguaacau ggcagaagua      780

cgcucauauu guuaugaagc aagcauaagc gauauggcau cagauucaag auguccaaca      840

caaggagaag cauauuuaga uaaacaaucu gacacacaau auguauguaa acguacguua      900

guagaccgug gaugggggaa cggaugugga uuauuuggua aaggaucguu aguaacaugc      960

gcaaaauucg caugcaguaa gaaaaugacc ggaaaaagca uacaacccga gaacuuagaa     1020

uauagaauaa uguuaagcgu acauggcagc caacauagug gaaugaucgu caacgauaca     1080

ggacaugaaa cagaugaaaa uagagcuaaa guagaaauaa ccccaaacag cccaagagca     1140

gaagcaacau uagguggauu ugguucacua ggauuagauu gcgaaccuag aacgggccug     1200

gauuuuagug accuauacua uuuaacaaug aauaauaaac acugguuagu ucauaaagaa     1260

ugguuucaug auauuccucu accauggcau gcaggcgcag auacagguac accacacugg     1320

aacaauaaag aagcauuagu agaguucaaa gaugcacacg caaagagaca aacugucgug     1380

guacuaggua gucaagaagg ugcaguucau acagcacucg caggagcguu agaagcagaa     1440

auggauggag cgaaaggaag auugaguuca ggucaucuaa aaugccgacu aaaaauggau     1500

aaauuaaggu uaaaaggugu aucauauuca uuaugcacag ccgcuuucac auuuacaaaa     1560

aucccagcag aaacuuuaca uggaacagua acuguagaag ugcaauaugc cggaaccgau     1620

ggaccaugca aagucccugc ucaaauggcu guagacaugc aaacauuaac gccaguugga     1680

agacuaauaa cagcaaaccc cguaauaacg gaaagcaccg aaaacagcaa aaugaugcua     1740

gaauuagauc caccuuuugg agacaguuau aucguuauag gcguaggcga aaagaaaauu     1800

acccaucauu ggcauagaag uggaagcaca auuggcaaag cauucgaggc aacaguaaga     1860

ggcgccaaaa gaauggcugu cuuaggagac acagcauggg auuuuggaag ugucggagga     1920

gcauuaaaua gccuuggaaa aggaauacau caaauauuug gggcagcauu caaaucacua     1980

uuuggaggaa ugagcugguu uucacaaauu uuaauaggua cccuauuaau gugguuagga     2040

cuaaaugcua aaaauggaag uauaaguuua augugucuug cauuaggagg aguauuaaua     2100

uuucuaucaa cggcuguauc agcugaugua ggauguagug uagacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4778
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 1 opt

<400>  4778
gggagaaagc uuaccaugag aauaaucaac gcucgaaaag aacgaaaaag aagaggagca       60

gauacuagua uuggcauaau aggucuauua cuuacuaccg cuauggcagc ugaaauaaca      120

agaagaggaa gcgcauacua cauguauuua gauagaagug augcaggaaa agcaauuucu      180

uuugcaacga cauugggagu aaauaaaugc cacguucaaa uaauggaucu aggacauaug      240

ugugaugcua cuaugucaua cgaauguccg auguuagaug aaggaguaga accagacgac      300

gucgauuguu gguguaauac aaccagcaca uggguagugu acggaacaug ccaccauaaa      360

aaaggagagg caagacgaag caggcgagca guaacauugc cgagucacuc aacaagaaaa      420

cuacaaacua ggucacaaac augguuagaa aguagagaau auacaaaaca uuuaauaaaa      480

guggaaaauu ggauauuuag aaauccagga uucgcucuag uugcuguggc aauagcuugg      540

uuauuaggua guaguacuag ucaaaaaguc auauaucuag uuaugauucu acugauagca      600

ccagccuauu cuauaagaug uauaggagua ucuaacagag acuuuguaga agguaugucg      660

ggaggaacuu ggguagaugu aguacuagaa cauggagguu guguaacggu cauggcacaa      720

gauaaaccua cuguagacau agaauuagua acaaccaccg uaucgaacau ggcagaagua      780

agaucuuauu gcuaugaagc aaguauuagc gauauggcaa gcgacucaag augcccuacu      840

caaggagaag cauacuuaga caaacagucc gauacucaau auguguguaa aagaacuuua      900

guagauagag gauggggaaa cggcugugga cuauuuggca aaggaucauu agugacaugu      960

gcaaaauuua cauguagcaa aaagaugaca gguaaaagua uacaaccaga aaauuuagaa     1020

uacagaauaa uguuaagugu acauggcucu caacauagcg gaaugauagg auaugaaaca     1080

gaugaggaua gagcaaaagu agaaguaaca ccuaauaguc cucgugcaga agcaacucug     1140

gguggauucg gaagccuagg auuggauugu gaaccacgaa caggauuaga uuuuagugau     1200

cuauauuacu uaacaaugaa uaauaaacau uggcuagucc auaaagaaug guuucaugac     1260

auuccauuac cauggcacgc aggugcagau accggaacac cacacuggaa uaauaaagaa     1320

gcuuuaguag aauuuaaaga cgcgcaugca aagaggcaaa cggugguagu auugggauca     1380

caagagggag caguacauac agcucuagca ggagcacuug aagcagagau ggacggugca     1440

aaaggaagau uauuuucagg acacuuaaaa uguagacuca aaauggauaa auuaagguua     1500

aaaggaguca gcuauagcuu augcacagca gcauuuacau ucacaaaagu accagcagag     1560

acauuacaug ggacaguuac aguugaagua caauaugcgg gaacagaugg accauguaaa     1620

auacccgugc aaauggcagu ugacaugcaa acauuaacac cuguaggaag auuaauuaca     1680

gcgaacccag uaauaacaga aucuacagaa aauaguaaaa ugauguuaga acuagaccca     1740

ccauuuggag auaguuauau agugauagga gugggugaua aaaaaaucac acaucacugg     1800

cauaggagug gaucuacuau uggaaaagcu uuugaagcaa caguaagagg agcaaaaaga     1860

auggcaguuu uaggagauac agcaugggau uucggaagcg uaggaggggu auuuaauagc     1920

cuagggaaag guauucauca aauauuugga gccgcauuua aaagucuauu cgguggaaug     1980

aguugguucu cacagauauu aauaggaaca uuauuagucu gguuaggauu aaauacaaaa     2040

aaugggucaa uaucucugac augucuagca cuagguggag uaaugauauu ucuaaguaca     2100

gcaguaagcg cagaugucgg auguagcgua gacuuuucga aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4779
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 1 opt

<400>  4779
gggagaaagc uuaccaugag aauaauaaau gcgagaaagg aaaaaaaacg acgaggagca       60

gauacaucag uuggaauagu aggcuuauua uuaacaacgg caauggccgc agaagugaca      120

agaagaggaa gugcauauua cauguauuua gauagaaacg augcuggaga agcuauaucu      180

uuuccaacca cauuaggaau gaauaaaugu uacauacaaa uaauggauuu aggucauaug      240

ugugaugcua caaugucaua ugagugucca auguuagaug aagguguaga accugaugac      300

guagauuguu ggugcaacac aacaaguaca uggguaguau auggaacaug ucaucauaaa      360

aaaggagaag ccagaagaag cagacgugcu guaacauuac ccucacauag cacaagaaaa      420

cuacaaaccc gaagucaaac gugguuagaa ucuagagaau acacaaaaca ucuuauaaga      480

guagagaauu ggauauuuag aaauccuggu uucgcauugg cagcagcagc aauugcgugg      540

cuacuaggau cgucuacaag ucaaaaagua auauacuuag uaaugauauu acuuauagcg      600

ccugcauaua guauaagaug uauaggagua agcaauagag auuuuguaga agguauguca      660

ggggguacgu ggguagaugu aguauuagaa caugguggau gcgucacagu aauggcacaa      720

gacaaaccua caguagauau ugaacuagua acaacaacag uaucaaauau ggccgaagua      780

agaucauauu guuaugaagc aucuauaagu gauauggcua gugacaguag auguccaaca      840

caaggugaag cauaucuaga uaaacaaagc gauacacaau auguguguaa aagaacauua      900

guagauagag gaugggguaa cggauguggu uuauuuggaa aaggauccuu aguaacgugu      960

gcaaaauuug cuuguagcaa aaaaaugaca ggaaaaucaa uccaaccaga aaauuuagaa     1020

uauagaauca uguuguccgu gcaugguagu caacacucug gaaugauugu aaaugauaca     1080

ggacaugaaa ccgaugaaaa uagagcaaaa guugaaauaa caccgaauag cccaagggca     1140

gaagccacau uaggaggauu uggcucguua ggucuagauu gugaaccaag aaccggauua     1200

gauuuuagug accuauauua ucuaacuaug aacaacaaac acugguuagu ucacaaagaa     1260

ugguuucacg auauacccuu acccuggcau gcuggggcag acacagguac accacauugg     1320

aauaauaaag aagcacuugu agaauucaaa gacgcucaug ccaagagaca aacuguaguu     1380

gugcucggua gucaggaagg ugcuguacac acagcacuug caggagcacu ugaagcagaa     1440

auggauggag caaaggguag auuaucuagu ggucauuuaa aauguagauu aaaaauggac     1500

aaauuaagau uaaaaggcgu aaguuauuca cuauguacug cagcauuuac auuuaccaaa     1560

auaccagcag aaacacuaca uggaacuguc acaguagaag uacaguaugc cggaacagau     1620

gguccaugua aagugccggc ucaaauggca guugauaugc aaacauuaac accaguagga     1680

agauuaauaa cagcaaaucc gguuauuaca gagucuacgg aaaauucgaa aaugaugcuu     1740

gaacuagacc caccauucgg ugacaguuac auugucauug guguagguga aaaaaaaaua     1800

acacaccauu ggcauagauc aggaaguaca auaggaaaag cauuugaagc uacagugaga     1860

ggagcgaaac gaauggcagu acucggugau acagcauggg auuuuggauc uguaggugga     1920

gcauuaaaua guuuaggaaa agguauacau caaauauuug gagcagcuuu uaaaucacua     1980

uuuggaggaa ugucaugguu uucucaaauu cucauaggua cacuauuaau guggcuaggc     2040

cuaaauacaa aaaacggaag cauaaguuua auguguuuag cuuuaggagg uguuuuaauu     2100

uuuuuaagua cagcgguauc agcagaugua ggauguucag uugauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4780
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 1 opt

<400>  4780
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag agcgaaaacg uagaggagca       60

gauacaagca uuggaaucau agguuuacua uugacaacag caauggcugc ugaaauaacu      120

agaagaggcu ccgccuauua uauguaccua gacaggucag acgcagguaa agccauaagc      180

uuugcaacaa cauuaggcgu aaauaaaugu cauguacaaa uaauggauuu aggacauaug      240

ugugacgcua caaugaguua ugaaugucca auguuagaug aaggagugga accagacgau      300

guugauuguu gguguaauac cacuaguaca uggguaguau augguacgug ucaccacaaa      360

aaaggcgaag caagaagauc cagaagagcg gucacauuac caucacauuc aacuagaaaa      420

cuacaaacaa gaucccaaac auggcuugaa agcagagagu acacuaaaca ccuaauaaaa      480

guagaaaauu ggauauucag aaacccugga uuugcauugg uagcaguagc aauagcaugg      540

uuauuaggaa guagcaccuc acaaaaagua auauaucuag uaaugauucu acuaauugca      600

ccagcauauu caauaagaug cauagguguu uccaauagag auuuuguaga aggaauguca      660

ggaggaacau ggguagacgu cguccuagaa cacggugggu gcguaacugu aauggcacag      720

gauaaaccaa cuguagauau agaauuagua acaacaacug uaucuaauau ggcagaagua      780

agaucauauu guuacgaagc aucaauaucu gauauggcaa gugauuccag augcccaaca      840

caaggagaag cauauuuaga uaaacaauca gauacacaau auguuuguaa aagaacccug      900

gucgaucgag guugggguaa uggauguggu cuuuuuggua aaggaagccu aguaacaugu      960

gcaaaauuua cauguagcaa aaaaaugaca ggaaaaucua uacaaccaga aaaucuugaa     1020

uauagaauaa uguuaagugu ucauggaagc caacauucag gcaugauagu gaaugauaca     1080

gguuaugaaa cagaugaaaa cagggcgaaa guagaaguaa caccaaacuc accacgagca     1140

gaagccacuu uaggaggauu cggaucauua ggacuagacu gugagccaag aacaggauug     1200

gauuuuagug aucuauacua uuuaacaaug aauaauaaac acugguuagu acacaaagaa     1260

ugguuccaug acauaccauu accuuggcac gccggagcag acacaggaac accccacugg     1320

aauaauaaag aagcgcuagu agaauuuaaa gaugcacacg ccaaaagaca aacugucgua     1380

guauuaggau cacaagaagg ugcaguacau acagcucucg caggagcauu agaagcagaa     1440

auggacggag ccaaagguaa acuuuuuuca ggacauuuaa aauguagauu aaaaauggau     1500

aaauugcgac uaaaaggugu aagcuauuca cuauguacag cagcauuuac auucacaaaa     1560

guacccgcag aaacacuaca uggaaccgug acaguagaag uucaauaugc aggaacagau     1620

ggcccaugua aaauaccugu acaaauggca guagauaugc aaacacuaac ucccgucggc     1680

agauuaauua cagcaaaucc aguaauaacu gaaaguacag aaaauucaaa aaugaugcua     1740

gaauuagauc cgccauucgg agauucauac auaguuauug gcgugggaga caaaaaaauu     1800

acacaucacu ggcauagaag cggaaguacc auaggaaaag cuuuugaagc aacaguucga     1860

ggagcaaaaa gaauggcagu auuaggugac acugccuggg auuucgguuc cguaggagga     1920

guuuucaauu cauuaggcaa agguauucac caaauauuug gugcggcauu caaaucauua     1980

uuuggaggua ugucaugguu uagucaaaua cuaauaggaa cauuacuagu augguuagga     2040

uuaaauacaa aaaacggguc cauaucuuua acauguuuag cucuaggugg cguuaugaua     2100

uuuuuaagua cugcaguaag ugcagauguu ggcugcucag ucgauuuuuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4781
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 1 opt

<400>  4781
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaagaaaaag aagaggggcu       60

gauacaagua uagguauagu aggacugcua uuaacaacgg caauggcugc agaaauaaca      120

aggaggggau cagcauauua cauguaucua gauagaucug augcgggaaa agcaauaucu      180

uuugcgacaa cuuuaggagu aaauaaaugc cauguacaga uaauggauuu aggacauaug      240

ugcgaugcga cuaugaguua ugaaugccca auguuagacg aagggguuga accagacgac      300

gucgauuguu gguguaauac aacgaguacu uggguaguau auggaacaug ccaucacaag      360

aaaggcgaag caagaagaag cagacgagcg guaacauuac caucacauuc aacaagaaaa      420

cuacaaacaa ggucacaaac auggcuagaa agcagagaau auacaaaaca uuuaaucaaa      480

guagaaaauu ggaucuucag aaauccaggu uuuacucuag uagccgucgc aauugcaugg      540

uuauuaggaa guaguacauc acaaaaagua auauauuuag uaaugauacu auuaauagcg      600

ccagcauaua guaucagaug uauaggagua agcaauagag auuuuguaga aggaauguca      660

ggagguacau ggguagacgu gguacuugaa cauggagguu guguuacugu aauggcacaa      720

gauaagccga cuguagauau agaauuaguu acaacaacug uaaguaauau ggcagaaguu      780

cgaaguuauu guuacgaagc aucgauaagu gauauggcau cagacucaag auguccuaca      840

caaggugaag cauaucuaga caaacaauca gauacacaau acguauguaa aagaacauua      900

guagaucgug gguggggaaa uggaugcgga uuguuuggaa aaggaagccu aguaacaugc      960

gcuaaauuua ccuguaguaa aaagaugacu ggaaaaucaa uucagccgga aaaccuagaa     1020

uacagaauaa ugcuaucagu acacggaagu cagcauucag guaugaucgu aaaugacgaa     1080

aauagagcua aaguagaagu gacgccuaac aguccaagag cagaagcuac gcuaggaggu     1140

uuugggaguc ucggauuaga uugugaaccu agaacaggau uggauuuuag cgauuuauac     1200

uacuuaacua ugaauaauaa acauuggcuu guacauaagg aaugguuuca ugacauacca     1260

uuaccauggc augcaggagc agauacaggu acaccccauu ggaauaauaa agaagcucua     1320

guugaguuca aggaugcaca ugcuaaaaga caaacagucg uaguauuggg gucacaagaa     1380

ggugcaguac auacagcucu ugcuggagca cuagaagcug aaauggaugg agcuaaagga     1440

agacuguuua guggucauuu aaaaugucga uuaaaaaugg acaaauuaag auugaaaggg     1500

guaagcuacu cuuuauguac ugcagcauuc acguucacua aaguaccggc ugaaacauua     1560

cauggaacag uaacaguaga aguacaauau gcggguacag auggaccaug uaaaguacca     1620

gcucaaaugg cgguagauau gcaaacacua acaccaguug ggagauugau aacagcgaac     1680

ccaguaauaa cggaaaguac agaaaauagu aaaaugaugu uggaacuuga cccaccauuc     1740

ggagauaguu auauaguuau cggaguagga gauaagaaaa uuacacauca uuggcauaga     1800

aguggaagua caauugggaa agcauuugaa gccacuguua gaggggcaaa aaggauggca     1860

guuuuaggag auacagcaug ggacuuugga agcguaggcg guguuuuuaa uagucuaggu     1920

aaagggauac aucaaauauu uggugcagca uucaaaaguu uauuuggagg gaugucaugg     1980

uucagucaaa uucuaauugg uacacuauua guaugguuag gucuaaauac gaaaaaugga     2040

aguauuucau ugacaugucu ugcauuaggu ggaguaauga uuuuccuaag uacagcuguu     2100

agugcagaug ugggauguag uguagauuuu ucaaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4782
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 1 opt

<400>  4782
gggagaaagc uuaccaugag aauaauaaac gcuagaaaag aaagaaaaag aagaggagca       60

gauacaucca uaggaauaau aggauuacuu cucacaacag cgauggcagc agaaaucaca      120

agacgcggaa gugcauacua uauguaucua gauagauccg augcaggaaa ggcgauauca      180

uuugcaacga cauuaggcgu uaauaaaugu cauguacaaa uuauggauuu gggccacaug      240

ugugaugcua ccaugaguua cgaauguccu augcuagaug aaggaguaga accugaugau      300

guggauuguu ggugcaacac aacaucaaca ugggugguuu augguacuug ccacagaaag      360

aaaggugaag caagaagaag cagacgagca guaacacuuc caucccauuc aacacggaaa      420

cuacaaacaa gaucacaaac augguuagaa ucuagagaau acacaaaaca ucuuauaaaa      480

guagaaaauu ggauuuuuag aaacccagga uuugcauuag uagcuguugc uauagcaugg      540

cuucuagguu caucaacauc acaaaaagua auuuauuuag uuaugauauu auuaauagcu      600

ccagcauaua guauuagaug uauuggagua ucgaauagag auuuuguuga agguaugagu      660

ggaggcacau ggguggacgu cguauuagaa cacgggggau guguaacagu aauggcacaa      720

gacaaaccaa caguugacau agagcuagua acuacaacag uaaguaacau ggcagaagua      780

agaagcuauu guuaugaagc caguaucagu gacauggcaa gugacaguag auguccuaca      840

caaggagagg cuuauuuaga uaaacaaagu gauacacagu auguauguaa aagaacauua      900

guagauagag guuggggaaa uggcugugga cucuuuggaa aaggaucgcu aguaacaugu      960

gcaaaauuua cauguucaaa gaaaaugacu ggaaaaucaa uucaaccaga aaacuuggaa     1020

uaccgaauaa ugcuaagcgu gcauggaucc caacauagcg ggaugauagu aaacgauaca     1080

ggauaugaaa cugacgaaaa cagagcaaaa guagaaguaa cuccaaauag uccaagagca     1140

gaagcaaccu uaggaggauu ugguucuuua ggacuagauu gcgaacccag aacaggauua     1200

gacuuuagug accuuuauua uuuaaccaug aauaacaaac auuggcuagu ucauaaagaa     1260

ugguuucaug auauaccauu accauggcau gcaggcgcag auacuggcac uccacauugg     1320

aacaauaaag aagcgcuagu agaauuuaaa gacgcucaug ccaagagaca aacagugguc     1380

guacuuggaa gccaagaagg cgcagugcau acagcucuag caggagcacu agaagcagaa     1440

auggauggug caaaaggaaa guuauuuagu ggacauuuaa aauguagacu aaaaauggau     1500

aaauuaagau uaaaaggagu cagcuacucg cucugcaccg cagcauuuac auuuacaaaa     1560

guaccagcag aaacauuaca uggaacaguu acaguugaag uacaauaugc agguacagau     1620

ggaccaugua aaauaccagu ucaaauggcu guagacaugc aaacauuaac gccuguaggg     1680

agacuaauaa cagcaaaucc agucauaacg gaaucaacag aaaacaguaa aaugaugcua     1740

gaacucgacc cuccauucgg agacucauac auaguaauug gaguuggaga caagaaaauu     1800

acucaccauu ggcacagauc agggucaaca auaggaaaag ccuucgaagc aacuguaaga     1860

ggagcuaaaa gaauggcugu auuaggcgac acagcauggg auuuuggauc cgucggagga     1920

guauuuaaua gcuuaggaaa aggaauacac caaauauuug gagcggcuuu uaaaucacuu     1980

uuuggcggaa ugucaugguu uucacaaaua cuuauaggaa cucuauuagu augguuaggc     2040

cuaaauacua aaaauggaag uauaucauua acaugucuag cauuaggagg agucaugaua     2100

uucuuaagca cagcaguaag ugccgacgua ggaugcucag uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4783
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 1 opt

<400>  4783
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaagaaaaag gagaggugcc       60

gauacgucua uagguauaau aggauuauua cuaacuacag caauggcggc agaaauaaca      120

cgaagaggaa gugcguacua cauguaucua gacagaagug acgcaggaaa agcaauuagc      180

uucgcaacaa cacuuggugu aaauaaaugu cauguccaaa uaauggauuu aggacauaug      240

ugugaugcca caaugucaua ugaaugucca augcuagaug aaggagugga gcccgaugac      300

guugacuguu gguguaauac aacaagcacu uggguaguau augguacuug ccaucauaag      360

aaaggugaag ccagaagaag cagacgagca guuacauuac caucacacag cacuagaaaa      420

uuacaaacaa ggagucagac uugguuagaa agucgagaau acacaaaaca ccuaauuaaa      480

guagaaaauu ggauauuuag aaacccuggc uucgcauuag uagcaguugc aauagcaugg      540

cuacuaggua guaguacaag ucagaaagua auuuauuuag ucaugauccu auugauagca      600

ccagcauauu caauaagaug uauugggguu ucaaauagag auuucguaga ggggaugagu      660

ggcggaacau ggguagacgu aguauuagaa cauggaggcu guguuacggu aauggcgcaa      720

gauaaaccaa cugucgauau agagcuagua acaacaacag uaucaaauau ggcagaggua      780

agaucuuacu guuaugaggc aaguauaagu gauauggcau cagacaguag auguccuaca      840

caaggagaag cauauuuaga uaaacaaucg gauacacaau auguauguaa aaggacgcua      900

guagaucgag gauggggaaa cggaugugga uuauucggaa aaggaaguuu aguuacaugc      960

gcaaaauuca cauguucaaa gaagaugaca ggaaaaucaa uacaaccaga aaaccuagaa     1020

uacagaauaa uguuaucugu acacggaagu cagcauucag gaaugacagu aaaugacaua     1080

ggauacgaga cugaugaaaa uagagcuaaa guagaaguaa cuccaaauuc accgagagca     1140

gaagcuacau uaggaggauu uggaagucua ggacuagacu gugaaccucg aacagguuua     1200

gauuuuagug auuuauauua cuuaacaaug aacaacaaac acuggcuagu ucauaaagaa     1260

ugguuucaug acauaccauu acccuggcac gcuggugccg auacaggaac cccacauugg     1320

aacaauaaag aagcauuagu agaauuuaaa gaugcacaug caaaaagaca aacaguagua     1380

gucuuggguu cacaagaagg cgccguacau acggcauuag caggggcuuu agaagcagag     1440

auggauggcg caaaagguaa auuauuuucg ggacaccuaa aaugcagauu aaaaauggau     1500

aaauuacgau uaaaaggagu aucauauuca cuauguacag cagcauuuac auuuacaaaa     1560

guaccagcug aaacucugca cggaacagug acgguagaag uucaauacgc cgguacagau     1620

ggaccaugca aaauaccugu acaaauggcu guagauaugc aaaccuuaac accaguagga     1680

agauuaauua cugcaaaucc aguaauuaca gagagcaccg agaauagcaa aaugaugcua     1740

gaauuagauc caccuuuugg ugauucauau auaguaaucg guguuggaga uaagaaaaua     1800

acacaccacu ggcauagauc aggaucaaca auagguaaag cauucgaagc aacaguaaga     1860

ggcgcaaaac gaauggcugu acuaggugau acagcuuggg auuuuggauc aguaggagga     1920

guauuuaaca gucuaggaaa aggaauacau cagauuuuug gggcagcauu caaaaguuua     1980

uuuggaggca ugagcugguu cagucaaaua uuaauuggaa cacuuuuagu auggcuagga     2040

uuaaauacga aaaaugguag uauaagucug acaugucuag cauuaggugg uguaaugaua     2100

uuucuuagca cagcggucag cgcagaugua ggauguucug uggacuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4784
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 1 opt

<400>  4784
gggagaaagc uuaccaugcg cauaauaaau gccagaaaag aaagaaaaag gagaggugca       60

gauacaucca uaggaauaau aggacuguug cuaacgaccg caauggcagc agaaauuaca      120

aggaggggcu cagcauauua uauguaucua gauaggucug augcaggaaa agcaauaagu      180

uuugcaacaa cacuaggggu aaauaaaugc cauguucaaa uaauggauuu aggacauaug      240

ugugaugcaa caaugaguua cgaaugcccu augcuagacg aaggagugga accggacgac      300

gucgauugcu gguguaauac aacuucaaca uggguuguau augguacuug ccaucauaaa      360

aaaggagaag caagaagaag ccgaagagca guaacacuac caagccauag cacucgaaaa      420

uugcaaacaa gaucucagac auggcuagaa ucaagagaau auacaaaaca uuuaaucaaa      480

guagaaaauu ggauauuuag aaauccagga uucgcccuag uagcaguggc aauagcaugg      540

uuauuaggau cuucaacaag ucaaaaaguc auauauuuag ugaugauuuu gcuaauagcu      600

ccagcauauu cuauaagaug uauaggagua ucaaacagag acuuuguuga aggaaugagu      660

ggcggaacau ggguagaugu aguauuagaa cauggaggau gugugacagu aauggcucaa      720

gauaaaccaa cuguugauau ugaacuugua acaacaacag uaucuaacau ggcugaagua      780

agaaguuauu guuaugaggc uucuauauca gauauggccu cagauaguag gugcccuaca      840

caaggagaag cauacuuaga uaaacaaagu gacacacaau acguauguaa acgaacuuua      900

guagauagag guuggggaaa uggcuguggu cuauuuggaa aaggaagucu aguaacaugu      960

gcaaaauuua cauguucaaa aaaaaugacu ggaaaaucaa uucaaccaga aaauuuagag     1020

uauagaauaa ugcuaucagu acauggaagu caacacagug ggaugauagg auacgaaaca     1080

gacgaaaaua gggccaaagu agaaguaaca ccgaacucuc caagagcgga agcgacauua     1140

gggggauucg gaucauuagg auuagauugu gaaccaagaa caggauuaga uuuuagcgau     1200

cuauauuacu uaacaaugaa uaacaaacau uggcuaguac auaaagaaug guuucaugau     1260

auaccucuac cauggcaugc aggagcagau acugguacgc cacauuggaa caauaaagaa     1320

gcauugguag aauuuaaaga ugcacaugcu aagaggcaaa cgguuguagu acucggauca     1380

caagaaggag caguacauac ugccuuagcu ggugcacuag aagcagaaau ggauggugca     1440

aaaggaaaac uauuuagugg acauuuaaaa ugucgacuaa aaauggacaa acuaagacua     1500

aaaggaguuu cauauucuuu augcacagcc gcauuuacau ucacaaaagu cccagcagaa     1560

acacuucacg gaacagugac aguugaagua caauaugcag gaacggaugg accguguaaa     1620

auaccuguac aaauggcagu agauaugcaa acacuaacac caguaggaag auugauaaca     1680

gcaaacccag uaauaacaga aucaacagaa aacucaaaaa ugauguuaga guuggauccg     1740

ccauucggag auaguuauau uguuauagga guaggcgaca agaaaauaac ucaucauugg     1800

cauagaucag gaagcacuau agguaaagcg uucgaggcaa cggugagagg ggcuaaaaga     1860

auggcaguau uaggagauac agcaugggau uuuggaagug uaggaggagu auucaacuca     1920

cuaggaaaag gaauacauca aauauuugga gcagcuuuua aaucacuauu cgguggcaug     1980

agcugguuuu cacaaauauu aauaggcaca cuauuaguau gguuaggacu aaacacaaaa     2040

aauggaagua uaagucuaac auguuuagca cuaggaggag uaaugauauu ucuaucaaca     2100

gcagugagug cugauguagg uugcaguguu gauuucucga aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4785
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 1 opt

<400>  4785
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaagaaaag acguggagcu       60

gauacaucag uaggaauagu aggauuacua uuaacaacug cgauggccgc agagguaaca      120

cgaagaggau cagccuacua uauguaucug gacagaaaug augcaggaga agcaauaagu      180

uuuccaacua cauuagguau gaauaaaugu uauauacaaa uuauggacuu aggacauaug      240

ugcgaugcua caaugucaua cgaaugccca auguuagaug aagguguaga acccgacgau      300

guugauuguu gguguaacac aacaaguaca uggguaguuu acgggaccug ucaccauaag      360

aaaggagaag cgagaagauc aaggagagca gucacucugc caagccacuc uacaagaaaa      420

uugcaaacaa gaagucaaac auggcuagaa aguagggaau acacaaaaca uuuaaucaga      480

guagaaaacu ggauauuuag gaauccaggc uuugcacuag cagcagcagc aaucgcuugg      540

uugcuaggua gcaguacaag ucagaaagua auauauuuag uaaugauuuu auuaauagca      600

ccagccuauu caauucgaug uauagguguc agcaauagag auuuuguaga aggaaugucu      660

ggcggaacau ggguugaugu aguacuugaa cauggaggau gugugacagu aauggcucaa      720

gacaaaccaa cuguagacau agaauuagua accacuacug uaaguaauau ggcagaagua      780

agaaguuauu guuaugaggc uucaauauca gauauggcau cagacucaag auguccaaca      840

caaggagaag cauaucuuga caaacaauca gauacgcaau auguauguaa aagaacucua      900

gucgauagag gaugggguaa cggaugugga uuauucggaa aagguagcuu gguaacuugu      960

gcaaaauuug cauguaguaa gaaaaugaca gggaaaagua uacaacccga aaaucuagaa     1020

uaucgaauaa uguuaagugu gcauggaucg caacauagug gaaugauagu aaaugauaca     1080

ggacaugaaa cagaugaaaa uagagcuaaa guagaaauaa caccaaauuc accgagagca     1140

gaagcaaccu uaggaggguu uggaaguuug ggauuagauu gugaacccag aacaggcuua     1200

gacuuuucag aucuauauua ccuaacuaug aacaauaaac auugguuagu acauaaagaa     1260

ugguuucaug auauaccuuu accauggcau gcaggagcag auacugguac accgcauugg     1320

aauaauaaag aagcauuagu cgaauuuaag gaugcacacg caaaaagaca aacaguagua     1380

guacuaggau cucaggaagg cgcagugcau acagcacucg cuggugcauu agaagcagaa     1440

auggauggag caaaaggaag auuaagcucg ggacaucuaa aauguagauu aaaaauggac     1500

aaauugagau uaaaaggugu aagcuacagu uuaugcacag ccgccuucac auucacaaaa     1560

auaccugcag aaacauuaca uggaaccguu acgguagaag uacaauacgc aggaacagau     1620

ggaccaugua aagucccagc acaaauggca guagauaugc aaacauuaac accaguaggu     1680

agauugauaa cugcaaaucc aguaauuacg gaaucgacag aaaauaguaa aaugaugcua     1740

gaauuagauc caccauucgg cgauaguuau auaguaauag gaguuggaga gaaaaagaua     1800

acacaucacu ggcaucgaag ugguucaaca auaggcaaag cauucgaagc aacagucaga     1860

ggagcaaaaa gaauggcugu auuaggcgac acagcguggg acuucggaag uguaggugga     1920

gcacuaaauu cauuaggaag aggaauacau caaauauuug gagcagcauu uaaaucacua     1980

uuuggaggua ugagcugguu uagucaaaua uuaauuggaa cauuauuaau gugguuagga     2040

cuaaauacua aaaaugguuc uauaaguuua auguguuuag cacuaggagg aguacuaauu     2100

uuucucagua cagcuguauc agcagauguu gguuguucag uugauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4786
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 1 opt

<400>  4786
gggagaaagc uuaccaugag aauaauaaac gcacgaaaag agaaaaagag aagaggcgca       60

gaugccagcg uaggaauagu aggauuacua cuaacaacug caauggcagc cgaagucacu      120

cgaagaggaa gugcauauua uauguauuua gauaggaaug augcagggga agcaauauca      180

uuccccacaa cauuaggaau gaauaaaugc uacauacaaa uaauggauuu aggacauaug      240

ugugacgcaa cgaugucaua cgaauguccu auguuagacg agggaguuga accagaugac      300

guagauugcu ggugcaauac aacaucaaca uggguuguau auggcacuug ucaccauaag      360

aaaggagaag cgagaagauc uaggagagca guaacacuac caucacauag uacgagaaaa      420

cuacaaacaa gaucucaaac augguuagaa ucaagagaau auacaaaaca cuuaauaaga      480

guagaaaacu ggauauucag aaacccagga uucgcauuag cagcagcagc aauugcgugg      540

uuacuaggaa guaguacaag ccaaaaaguu auauaucuug ucaugauucu auuaauagca      600

ccagcauaua guauuaggug cauaggcguu agcaacagag auuucguaga agggauguca      660

ggaggaacau ggguagaugu uguauuagag caugggggau gugucaccgu aauggcacag      720

gauaaaccua caguagacau cgaauuaguu acaacaacag uaucaaauau ggcagaagua      780

agaucuuauu gcuaugaagc aucaauauca gacauggcua gugauagccg augcccuaca      840

caaggagaag cauacuuaga uaaacaauca gauacacaau auguauguaa aagaacccua      900

guagauagag gauggggaaa uggaugugga uuauucggaa aaggaucauu gguaacuugu      960

gcaaaauuug cauguucaaa gaaaaugacu ggaaaaagca uacagccuga aaaucuugaa     1020

uacagaauaa ugcuuagugu acacggaucu caacauucag ggaugauagu aaacgauaca     1080

gggcaugaaa cagaugaaaa uagagcgaaa guagaaauua caccuaauag ucccagagcu     1140

gaagcaacac uaggaggauu cggaagccua ggacuugauu gugaaccccg uacaggauua     1200

gauuuuucag acuuauauua uuugacaaug aacaauaaac auuggcuagu ccacaaagaa     1260

ugguuccacg acauaccgcu acccuggcac gcaggagcgg acacugguac cccacauugg     1320

aauaauaaag aagcauuagu agaguucaaa gaugcacaug cuaaacgaca aacaguagua     1380

guacuuggaa gucaagaagg agcaguacau acagcauuag cgggugcacu agaagcagaa     1440

auggauggag caaaaggaag auuaaguagu gggcaucuaa aaugcagacu caaaauggau     1500

aaacuaaggu uaaaaggagu aucauauuca uuauguacag cagcuuuuac auuuacuaaa     1560

auaccugcag aaacguuaca uggaacggua acugucgaag uacaauaugc uggaaccgac     1620

ggaccaugca aaguaccugc acaaauggca guagauaugc aaacauuaac uccuguaggc     1680

agauugauaa cugcaaaccc ugucauaacg gaaucaacag aaaauaguaa aaugaugcuu     1740

gaauuagacc caccauuugg agauaguuau auaguaaucg gaguagggga aaagaaaaua     1800

acacaucacu ggcacagguc ggggucaaca auaggaaaag cguuugaagc aacaguaaga     1860

ggagcaaaaa ggauggcagu auuaggugac acagcuuggg auuuuggaag cguagguggu     1920

gcauuaaaua guuuaggaaa aggcauucau caaaucuucg gagcggcauu uaaaagucua     1980

uucggaggaa ugucuugguu uucucaaaua cuuauuggaa cuuuauuaau guggcuagga     2040

uuaaauacua aaaacgguag uauaaguuua augugccucg cauugggagg aguauuaaua     2100

uuucucagua cagcgguaag cgcagacgua ggguguagcg uagauuucuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4787
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 1 opt

<400>  4787
gggagaaagc uuaccaugag aaucauaaau gcucggaagg aaaaaaaaag aagaggagcc       60

gauacaagcg uaggaauagu aggauuacuu cuaacgacag cgauggcggc agaggucaca      120

aggagaggau ccgcauauua cauguacuug gauagaaaug augcaggaga agcaauauca      180

uuuccaacaa cacuaggaau gaacaaaugu uauauacaaa uaauggaucu aggacauaug      240

ugcgaugcua caaugucaua ugagugccca auguuggaug aaggaguaga gccagaugau      300

guagacuguu gguguaacac aacaucaacu uggguuguau acgggacgug ucaucauaaa      360

aaaggggagg cgaggagguc gagaagagca guaacguuac caucacauag cacaagaaaa      420

uuacaaacac gaagucaaac cuggcuagaa aguagagaau auacaaaaca uuuaauuaga      480

guagaaaauu ggauauuuag aaacccagga uuagcguuag cugcagcagc aauagcaugg      540

cuuuuaggua gcucgacaag ucaaaaaguu auauaucucg uuaugauacu cuuaauagca      600

ccugcauaua guauaagaug uauuggagua aguaaucgag acuuuguaga agguauguca      660

ggaggaacau gggucgaugu gguacuggaa cauggaggau guguaacugu uauggcacaa      720

gauaaaccaa caguagacau agaguuagua acaacaaccg uaucaaacau ggcagaagug      780

cguaguuauu gcuaugaagc gucgauauca gacauggcaa gugauagccg auguccaacu      840

caaggagagg cauauuuaga uaaacaaagu gauacucaau auguaugcaa aagaacacua      900

gucgauagag guugggguaa cggaugcgga uuauucggga aaggcucauu aguaacuugu      960

gcuaaauuug cuuguucaaa aaaaaugacu ggaaaaagca uucaaccaga gaauuuggaa     1020

uaucguauaa uguuaagugu ucauggauca caacacucag guaugauagu caaugauaca     1080

ggacaugaaa cagacgaaaa uagggcaaaa guagaaauaa caccaaauag uccuagagca     1140

gaagcaacau uaggcggauu ugggaguuua ggcuuagacu gugagccaag aacuggauua     1200

gacuuuagcg acuuauauua cuuaacaaug aacaacaaac auugguuagu acacaaagag     1260

ugguuucaug auauaccguu accauggcau gcaggagcag acacuggaac accacauugg     1320

aauaauaaag aggcauuggu agaauuuaaa gaugcccaug cgaaacgaca aacaguggua     1380

guacuaggau cacaagaagg cgcaguacau acagcacuug cuggcgccuu ggaagcagaa     1440

auggacgggg cuaaagguag auuaaguuca ggucauuuaa aauguagauu aaaaauggac     1500

aaauuaagau uaaaaggugu gucauauuca uugugcacag cagcauucac auuuacuaaa     1560

auaccagcug aaacauuaca cgguacagua acaguagaag uucaauaugc agguacggac     1620

ggcccaugua aagucccagc ucaaauggcg guagauaugc agacauuaac uccaguuggu     1680

agacuaauaa cagcaaaucc uguaaucacc gaaaguacug aaaauucaaa aaugauguug     1740

gaacuugauc caccauuugg agauucauau auuguaauag guguagggga aaaaaaaaua     1800

acacaucauu ggcacagaag uggaaguacg auuggaaaag ccuuugaagc aacgguaagg     1860

ggugcaaaac gaauggcagu auuaggugau acagcauggg auuuuggcag uguaggaggu     1920

gcacuaaauu cauuaggaaa aggaauacau caaauauucg gagcagcauu uaaaagcuug     1980

uuugguggua ugucaugguu uucacaaaua cuaauaggaa cacuauuaau gugguuagga     2040

uuaaauacaa aaaaugggag uauaagucuc augugucugg cauuaggcgg aguacucaua     2100

uuuuuaagua cagcaguuag cgcagaugua ggauguagug uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4788
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 1 opt

<400>  4788
gggagaaagc uuaccaugcg aauaauaaau gcacgaaaag aaaagaaaag gagaggagcu       60

gacacuuccg uaggcauugu aggauuacuc uuaacaacug cuauggcagc ugaagucacc      120

agaagagggu cagcauauua cauguaucuu gauagaaaug acgcagggga agcaauuagc      180

uuuccaacaa cacuuggaau gaauaaaugu uacauacaaa uaauggaccu cggacauaug      240

ugcgacgcaa cuaugagcua ugaaugucca augcuagacg aaggaguaga accagaugac      300

guagacuguu ggugcaauac uacaaguacu uggguaguuu augggacaug ccaucauaag      360

aaaggugaag caagaagauc uaggagagca guuacauuac cuagucauag cacgagaaaa      420

cuacaaacaa gaucccaaac augguuagaa ucaagggaau acacaaaaca ucuuauaaga      480

guagaaaauu ggauauucag aaaucccggu uucgcucuag cagcagcugc uauagcuugg      540

cuauugggaa guagcacuag ccaaaaagua auauacuuag uaaugauacu auuaauagcu      600

ccugcauauu cuauaagaug uauuggagua aguaacagag acuucguuga aggaaugagu      660

ggaggcacau ggguagaugu cguacuugaa cacggaggau guguaacagu aauggcacaa      720

gauaaaccga caguagauau ugaauuggua acaacaacag ucaguaauau ggcagaaguu      780

agaaguuauu guuaugaagc uaguauaagu gacauggcau cagauucacg auguccuaca      840

caaggagagg cuuaucuaga caaacagucu gauacacaau acguguguaa aagaacuuua      900

guugaucgag gauggggaaa uggaugugga cuauuuggaa aaggaucauu aguaacaugu      960

gcaaaauucg cauguucaaa gaaaaugaca ggaaaaagca uacaaccaga aaauuuagaa     1020

uacagaauaa uguuaagugu gcauggaucc caacacagcg gcaugauagu aaaugauaca     1080

ggacacgaaa cagaugaaaa cagagcaaaa guugaaauua caccaaauag uccacgagcg     1140

gaagccacac uuggaggguu cggaucacua ggacuugacu gugaaccaag aacuggauua     1200

gauuucucag auuuauauua cuuaacaaug aauaacaaac auugguuggu acauaaagaa     1260

ugguuucacg auauuccgcu accauggcau gcuggagcag auacagggac accacauugg     1320

aauaacaaag aagcucuagu agaauuuaaa gaugcacaug caaagagaca aacaguagua     1380

guacuaggaa gucaggaagg agcaguacau acagcacuag caggagcucu agaagcugaa     1440

auggauggug caaaaggaag acuaaguagc ggacaucuaa aauguagacu aaaaauggac     1500

aaacuaagac uaaagggggu uucauauuca uuauguacag cagcuuuuac auucacaaaa     1560

auaccugcag aaacuuuaca ugguacagua acaguugaag uacaauaugc uggaacagac     1620

ggacccugua aaguaccagc acaaauggca guagauaugc aaacauuaac accaguugga     1680

cgauuaauaa cagcaaaucc cguuauaacc gaaaguacag aaaauucuaa aaugauguua     1740

gaauuagauc ccccauucgg agacagcuau auagugauug gaguaggaga aaagaaaauu     1800

acacaucacu gguaccgcuc aggaucaaca auaggaaaag ccuucgaagc uacuguaaga     1860

ggugcaaaac gaauggcagu acuaggggac acagcguggg auuucgguuc aguaggagga     1920

gcacuuaacu ccuuagguaa aggcauacau caaauauuug gagcagcuuu uaaaucucua     1980

uuugggggca ugucaugguu cucacaaauu uuaaucggca cacuacuaau gugguuagga     2040

cuaaauacua aaaacggauc aauaagucuc augugucuag cccuaggagg aguacuaaua     2100

uuucuaagua cugcuguauc agccgaugug ggaugcagug uagauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4789
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 1 opt

<400>  4789
gggagaaagc uuaccaugag aauaauuaau gcacgaaaag aaaaaaaaag aagaggagcu       60

gauacaagug uaggcauagu agguuuacug uuaacuaccg cuauggcagc agaaguaacg      120

agaagaggau cagcauauua uauguauuua gauagaaaug augcugggga agcaauauca      180

uuuccuacaa cauuaggaau gaauaaaugc uauauucaaa uaauggaucu gggacacaug      240

ugugaugcaa caaugucaua ugaaugcccc auguuagaug aagguguaga accagaugau      300

guagacuguu gguguaauac aacaucaacu uggguaguau auggaacaug ccaucauaaa      360

aaaggagaag cgagaagaag cagaagagcu guaacauuac caucacauag uacuagaaaa      420

cuacaaacaa gaagccaaac auggcuugaa aguagggaau auacuaaaca ucuuauaaga      480

guagaaaauu ggauauuuag aaauccagga uucgcauuag cagcugcagc uauagcaugg      540

cuauuaggau cauccacaag ucaaaaagua auauauuugg uaaugauauu acuaauugca      600

ccagcauacu caauaagaug uauaggaguc aguaauagag auuuugugga aggaaugagc      660

ggaggaacau ggguagacgu aguuuuagaa cacgguggau gcguuacagu uauggcacaa      720

gauaaaccaa cuguagacau agaauuaguu acaaccacag uaaguaauau ggcagagauu      780

agaucauauu guuaugaagc auccauaagc gauauggcau cggauaguag auguccaaca      840

cagggcgaag cauaccuaga uaaacaaagc gauacgcaau auguuuguaa aagaacgcua      900

guagauagag gaugggguaa ugguugugga cuuuucggaa aaggaagccu gguaaccugu      960

gcaaaauucg cauguucuaa aaaaaugaca ggaaaaucua uucaaccaga aaaucuggaa     1020

uauagaauaa uguuaagcgu ccauggaagu caacauuccg gaaugauugu aaaugauacg     1080

ggacaugaaa cagacgaaaa cagagcaaaa guugaaauaa caccaaauuc cccaagagca     1140

gaagcaaccc uaggaggauu uggaucauua ggauuagauu gcgaaccuag aacaggacua     1200

gacuucucag accuguauua ccuaacaaug aacaauaaac auugguuagu acauaaagaa     1260

ugguuccaug auauaccacu accguggcau gcaggagcug acacuggaac accccauugg     1320

aauaacaaag aagccuuagu agaguuuaaa gaugcacacg caaaacgaca aacaguaguc     1380

guccuaggua gucaagaagg ugcuguacau acagcauuag cuggugcucu agaagcagaa     1440

auggauggag cuaaaggaag acuaucaagu ggacaccuaa aaugccgauu aaaaauggau     1500

aaauuaagac uaaaaggagu aaguuacagu uuaugcacag cggcauuuac auuuacaaaa     1560

auacccgcag aaaccuuaca ugguacagua accguagaag uacaauaugc aggcacggau     1620

ggaccaugua aaguaccagc acaaauggcu guugauaugc aaacacuaac accgguaggc     1680

cgauuaauaa cugcuaaccc aguaaucaca gaaaguacug aaaauaguaa gaugauguua     1740

gaauuggacc caccauuugg agacucauau auaguaaucg gcguaggaga aaaaaaaauu     1800

acacaucacu ggcauagauc aggaaguaca auaggaaaag ccuucgaagc aacuguaaga     1860

ggagcaaaga gaauggcagu acuaggagau acagcauggg auuuuggauc gguuggagga     1920

gcucucaaua gcuugggaaa aggcauacau caaauauuug gagcagccuu uaaaaguuug     1980

uucggaggaa ugaguugguu uucucaaaua uuaaucggga cacuacuaau guggcuggga     2040

uuaaauacaa aaaauggcuc aauaagucuc augugcuuag cuuuaggggg cguuuuaaua     2100

uuuuuaagua cagcaguauc agcagacguu gguugcagcg uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4790
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 1 opt

<400>  4790
gggagaaagc uuaccaugag aauaauaaau gcccgaaaag aaaaaaaacg aagaggagcc       60

gacacaagug uaggaaucgu aggguuauua cuuacgacgg cuauggcggc agaaguaaca      120

cgaagaggaa gugccuacua cauguacuug gacagaaaug acgcaggaga agcaauauca      180

uuuccaacaa cacuagguau gaacaaaugu uauauucaaa ucauggaucu aggucauaug      240

ugugaugcca cuaugagcua cgaaugccca augcuugaug agggcguaga accugaugau      300

guggacuguu ggugcaacac aacuaguacu ugggucgugu acggcacaug ucaucauaaa      360

aaaggagaag caagaagaag caggagagca guaacguuac cgagccauuc aacaagaaaa      420

cuacaaacaa gaagccaaac augguuagag agucgagaau auacaaaaca cuugauaaga      480

guagaaaacu ggauauuuag aaauccaggu uucgcauuag cagcagcagc aauugcaugg      540

cuauugggua guuccacaag ucaaaaagua auuuauuuag uuaugauauu auuaauagcu      600

cccgcauacu caauaagaug cauaggagua agcaauagag auuuuguaga gggaaugucc      660

ggaggaacgu ggguagaugu agucuuagaa caugguggau guguaacugu aauggcacaa      720

gauaaaccaa cuguagauau cgaacuugua accacaaccg uaucaaauau ggcagaaguu      780

agaucguacu gcuaugaggc cucaauauca gauauggcau cagauucaag auguccaaca      840

caaggugaag cauaucuuga caaacaaucu gauacucaau auguauguaa aaggacacua      900

guagauagag guugggguaa uggcugugga cuauuuggua aaggaucucu aguaacaugu      960

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaagua uacaaccaga aaaucuagaa     1020

uacagaauaa uguuauccgu acaugggagc caacauagcg gaaugauagu aaaugauacu     1080

ggacacgaaa cagaugaaaa ucgagcaaaa guagaaauaa cucccaauuc accuagagcu     1140

gaagcaaccc uaggagguuu uggaaguuua ggauuagauu gugaaccacg aacaggucua     1200

gauuuuucag aucuauauua uuuaacaaug aauaacaaac acugguuagu acauaaagaa     1260

ugguuucaug auauaccauu accauggcac gcaggggcag acacaggaac uccccacugg     1320

aacaacaaag aagcuuuagu ggaauuuaaa gaugcacaug caaaaagaca aacaguagua     1380

guacuaggcu cucaagaagg agcugugcau acugcauuag caggagccuu agaagcagaa     1440

auggauggag caaaagggag auugucauca gggcauuuaa aaugucgacu aaaaauggau     1500

aaauuaagau uaaaaggggu cagcuauuca cuauguacgg cagcuuuuac auuuacaaaa     1560

auaccagcag aaacacucca ugguacagua acaguagaag uucaauaugc agguacagau     1620

ggaccaugua agguucuagc acaaauggca guagauaugc aaacguuaac uccuguagga     1680

agauuaauaa cagccaaucc uguaauaaca gaaucaaccg aaaauucaaa aaugauguua     1740

gaauuagauc cuccuuuugg agauucuuau auaguaauag guguugguga aaaaaaaaua     1800

acacaucauu ggcauagaag uggaagcaca auagguaaag cauuugaagc aacaguaaga     1860

ggagcaaaac gaauggcugu acuaggagac acagcuuggg auuucggaag uguaggcgga     1920

gcauuaaauu cauuaggaaa ggguauacau caaaucuuug gggcugcauu caaaaguuua     1980

uuugguggaa ugagcugguu cucacaaauu cuuaucggaa cacuauuaau guggcuaggu     2040

cuuaauacaa aaaauggguc aauaucuuua auguguuuag cauuaggggg aguacuuaua     2100

uuccucucaa cagccguauc agcagacguu ggauguucag uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4791
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 1 opt

<400>  4791
gggagaaagc uuaccaugag aauaauaaau gccagaaaag aaaaaaaaag acguggugcu       60

gacaccagug uaggaauagu aggauuauug uuaacaacug caauggcggc agaaguaaca      120

agaaggggaa gugcauauua uauguaucua gauagaaacg acgcaggcga agcaauaagu      180

uucccaacaa cauuaggaau gaacaaaugu uauauacaaa uaauggaccu cgggcacaug      240

ugcgaugcaa caaugaguua ugaaugccca acacuagacg aaggaguuga accggaugac      300

guagauuguu ggugcaacac aacaaguacg uggguuguau augguacgug ucaucauaaa      360

aaaggugaag ccaggagguc aagaagggca guaacauugc caucccauag uacacggaaa      420

uugcaaacaa gaucacagac uuggcuugaa agcagagaau auacaaaaca uuuaauaaga      480

guagaaaauu ggauauuucg gaauccagga uuugcauuag cagcugcugc aauugcaugg      540

cuucuagguu cgucaacauc acaaaaagua auauauuugg uaaugauauu guuaauugcu      600

ccagcauauu caauucguug uauaggugua agcaaucgug auuucguaga aggaaugagc      660

ggagggacgu ggguugaugu cguguuagaa cauggagggu guguaacagu aauggcacaa      720

gauaaaccaa caguagauau agaauuagua acaacuacag uaagcaacau ggcagaagua      780

agaucauauu guuaugaagc aaguauaucc gacauggccu ccgauaguag augucccaca      840

caaggugaag cguauuuaga uaagcaaucu gauacacaau acguaugcaa aagaacacua      900

guagauagag guuggggaaa uggcugcgga uuauuuggaa aaggcagucu aguuacaugc      960

gcaaaauuug cauguagcaa aaaaaugaca ggaaaaagua uucaaccaga aaacuuagag     1020

uauaggauaa uguuaucggu acacggaagu caacacucag gaaugauugu aaacgacacg     1080

ggccacgaaa cugacgaaaa uagagcaaaa guagaaauca cgccaaauuc accacgagcc     1140

gaagccacau uaggaggauu uggaucacug ggauuagauu gcgaaccuag aacgggauug     1200

gauuuuagug auuuauauua uuuaacuaug aauaauaaac auugguuagu acauaaagag     1260

ugguuucaug auauaccucu accuuggcau gccggggcag auacuggaac uccacauugg     1320

aauaauaaag aagcauuagu agaauuuaaa gaugcccacg caaaaagaca aacaguaguu     1380

guauuaggaa gucaggaagg agcuguacau acagccuuag caggagcacu agaagcagaa     1440

auggauggag caaaaggaag auuaucaagu ggacacuuaa aauguagauu aaaaauggac     1500

aaacuaagac uuaaaggugu gucauacucc uuauguacag ccgcuuuuac uuuuacuaaa     1560

auuccagcgg agacuuuaca ugggacugua acaguagaag uucaguaugc agguacagau     1620

gguccaugca aaguaccagc ccaaauggca guugauaugc aaacauuaac accaguagga     1680

agauuaauca cagccaaucc cguaaucaca gaauccacag aaaauaguaa gaugaugcua     1740

gaauuagacc caccguuugg agauaguuau auaguaauag gaguugggga aaagaaaaua     1800

acacaucauu ggcaccgaag cgguagcacc auaggaaaag cuuuugaagc aacaguuaga     1860

ggagcuaaaa gaauggcagu auugggugau acagcauggg auuucggauc aguaggugga     1920

gcauuaaaca gcuuggguaa aggaauacau caaauuuucg gagcagcauu uaaaagcuua     1980

uuuggaggaa ugaguugguu cucacaaaua uuaauaggaa cacuauuaau gugguuagga     2040

cuaaauacaa aaaauggaag uauaaguuua auguguuuag cauuaggggg aguacuaaua     2100

uucuuaucaa cagcaguauc ugcugacgua ggaugcucag uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4792
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 1 opt

<400>  4792
gggagaaagc uuaccaugag gauaauaaau gccagaaaag aaaagaaaag gagaggagca       60

gacacaagcg uaggaauagu aggcuuauua uuaacaacag caauggccgc agagguaacu      120

cgaagaggau cagcguacua uauguauuua gauagaaaug augcaggaga agcaauauca      180

uuuccaacaa cauuaggaau gaacaaaugc uacauacaaa uaauggauuu agggcauaug      240

ugugaugcca cuaugaguua ugaaugccca augcuagacg agggugugga accagaugac      300

guagauuguu gguguaacac aacaucuaca uggguuguau auggaacuug ucaucacaag      360

aaaggagaag cacgaagaag caggagggca guaacacuac caagccauuc aacuagaaaa      420

cuccaaacaa gaagucaaac cugguuagaa ucaagagaau auacgaaaca ucuaauaaga      480

guagaaaacu ggauauuuag aaaucccgga uuugcauuag cagcagcagc aaucgccugg      540

cuauuaggaa guucaacaag ucaaaaagug auauaucuag ucaugauacu acuaauugca      600

ccagcuuaua gcauaagaug uaucgguguc agcaauagag acuucguuga agguauguca      660

gguggaacgu ggguagacgu uguuuuagaa cacggcggau guguaacagu aauggcacaa      720

gauaaaccaa cgguagacau agaacuugug acaacuacug uuaguaacau ggcagaagua      780

cgaagcuauu gcuaugaagc aucuauauca gauauggcau cagauucacg auguccuaca      840

caaggagaag cauacuuaga uaaacaaagc gauacacaau auguuuguaa aaggacauua      900

guagauagag gcuggggaaa ugguugugga uuauuuggaa aagguucauu gguaacgugu      960

gcaaaauucg cuuguagcaa gaaaaugaca gguaaaucaa uacaaccuga aaacuuggaa     1020

uacagaauua ugcuaucagu acauggauca caacauuccg gaaugauagu aaaugauacu     1080

ggucaugaaa cagaugaaaa ccgagccaaa guagaaauaa caccuaauuc uccaagagca     1140

gaagcuacau uaggaggauu cggaucacuu ggucuagauu gcgaaccaag aacuggacuu     1200

gacuuuagug auuuauacua uuuaacaaug aauaauaaac auuggcuagu acauaaagaa     1260

ugguuucaug acauaccauu accauggcac gcaggagcag auaccggaac accacacugg     1320

aacaauaaag aagcucuagu agaguucaaa gaugcucaug caaaaagaca gaccguggua     1380

guauuaggau cacaagaagg agcuguacau acggcucuag caggagcacu ugaagcggaa     1440

auggauggug caaaaggacg auuaaguuca gggcaucuaa aauguagauu aaaaauggac     1500

aaauuaagau uaaaaggagu aaguuauagu uuaugcacag ccgcuuuuac auuuacuaaa     1560

auaccagcag aaacguuaca uggaaccguu accguagaag uacaauaugc aggaacagau     1620

gguccaugca agguaccagc acaaauggca guagauaugc aaacgcuaac accaguagga     1680

agguuaauca ccgcuaaucc agugauaaca gaaucgaccg aaaauuccaa aaugauguua     1740

gaauuggauc caccuuuugg agacuccuau auaguaauag ggguugggga aaagaaaaua     1800

acccaccauu ggcauagauc agggucaaca auaggaaagg cuuucgaggc aaccgugcga     1860

ggagcaaaaa gaauggcggu uuuaggggau acugcauggg auuuuggaag ugugggagga     1920

gcauuaaaca gccuggguaa agguauacau caaauauucg gagcagcauu caaaucauua     1980

uuuggaggaa ugagcugguu uagucagaua cuaauuggaa cauuacugau guggcuugga     2040

uuaaacacca aaaacggauc aauuagcuua augugcuuag cacuuggugg aguauuaaua     2100

uuccuaagua caguaguuuc agcagaugua gguugcucag uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4793
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 1 opt

<400>  4793
gggagaaagc uuaccaugag aauuauaaau gccagaaaag aaaaaaaaag aagaggcgcc       60

gauacaucag uagggauagu aggauugcua uuaacaacag ccauggcagc agaaguuaca      120

agacgaggau cagcauauua cauguacuua gauagaaaug augcaggaga agcuauaagu      180

uucccgacua cuuuaggaau gaacaagugu uauauucaaa uuauggacuu aggacacaug      240

ugugacgcaa caaugucaua ugaaugccca auguuagacg aaggaguaga accagaugau      300

guagauuguu gguguaacac uacaagcgcu ugggucgucu acgggacaug ccaccacaaa      360

aaaggagaag cuagacgaag uagaagagca guaacccuac caucucauag uacacgaaaa      420

cuacaaacua gaccucaaac cugguuagaa ucaagagaau acacaaaaca uuuaauacga      480

guagaaaauu ggauauucag aaacccagga uucgcguugg cagcggcggc aauugcaugg      540

uuauuaggau caaguacauc ucaaaaaguc auauauuugg uuaugauacu uuuaauagcu      600

ccagcauaua gcauacgaug uauaggagua agcaauagag acuuugugga aggaauguca      660

ggagggacau ggguugacgu agucuuagaa cauggaggau guguaacggu cauggcacaa      720

gauaaaccaa caguugauau agaauuagua acaacaacag uaucaaauau ggcagaaguu      780

agaucauauu guuaugaagc aucaaucuca gacauggcaa gugacucaag augucccacg      840

caaggugaag cauaucuaga caaacaaagu gauacacaau auguaugcaa aagaacacuu      900

guagacagag gauggggcaa cggaugcggu cucuuuggua agggcagcuu aguuacaugu      960

gcaaaauucg cguguaguaa aaaaaugaca gguaaaucaa uacagccuga aaaucuugaa     1020

uaucgaauua uguuaucugu ucacggauca caacauucug gcaugauugu aaaugacaca     1080

ggucacgaaa cagaugaaaa cagagcuaaa guagaaauaa caccgaacuc accaagagca     1140

gaagcuacuu uaggagguuu ugggaguuua ggacuagauu gcgaaccaag aacaggacuu     1200

gauuuuagug auuuauacua uuuaaccaug aauaauaaac auuggcuggu acauaaagaa     1260

ugguuucaug auauuccauu accauggcau gcaggagcug auacaggaac accccacugg     1320

aauaauaaag aagcgcuugu agaauuuaaa gacgcacaug ccaaaagaca aacaguagua     1380

guuuuaggua gucaagaagg ggcaguccau acagcacuag cgggagcauu agaagcagaa     1440

auggauggag caaaaggacg auuaagcuca ggacaucuaa aauguagacu gaaaauggau     1500

aaauuaaggc uaaaaggagu aagcuauagu cuuugcacag cagcauucac uuuuacaaaa     1560

auuccagcgg aaacacuaca uggaacugua acaguagaag ugcaauacgc aggaacugau     1620

ggaccgugua aaguuccagc acaaauggca guagauaugc aaacauuaac accaguagga     1680

cguuuaauaa cagccaaucc aguaauaaca gaaucaaccg aaaacucgaa aaugaugcua     1740

gaauuagauc cuccuuuugg agauucguac auaguuauag gugucggaga aaaaaagauc     1800

acacaucacu ggcauagaag cggcaguaca auaggaaaag cauuugaagc uaccguacgu     1860

ggagcuaaaa gaauggcagu ucuaggcgau acugcauggg auuuuggcuc aguuggaggu     1920

gcauuaaaca gcuuagggaa aggaauacau caaauuuuug gugcagcauu caaaagcuug     1980

uuuggaggaa ugucaugguu uagccaaaua uuaauuggaa cuuuacuuau gugguuagga     2040

cuaaauacaa aaaaugguag uauaucauua augugucuug cauuaggugg aguauugauu     2100

uuuuuaucaa cagcaguaag cgcagaugua ggauguucag uagacuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4794
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 1 opt

<400>  4794
gggagaaagc uuaccaugag aauaauaaau gcacgaaaag agaaaaagag acgaggcgca       60

gacacaagug uaggaauagu aggccuacuu uuaacuacag caauggcugc agaaguaacg      120

agacgagguu ccgcauauua uauguaccuu gacaggaaug acgcagggga agcaauaucc      180

uuuccaacca cauuaggaau gaauaaaugu uacauacaaa uaauggaucu aggacauaug      240

ugugaugcaa caaugaguua ugaaugucca augcuggaug aaggaguaga acccgaugac      300

guagauuguu ggugcaauac uacaucaaca uggguaguau augggaccug ucaucauaaa      360

aaaggagaag caagaaggag uagaagagca gucacauugc cgagucacuc aacaagaaaa      420

uuacaaacaa gaucccaaac auggcuugaa ucuagagaau acacaaaaca ucuuauaaga      480

guugaaaauu ggauuuucag aaaccccgga uucgcgcuag cagcagcagc aauagcaugg      540

cuccuuggau cauccacaag uaaaaaagua auauacuuag ugaugauauu acuaaucgca      600

ccagcauaca guauacguug cauaggagua ucaaaucgag auuucguuga agggaugucu      660

ggagguacau ggguagaugu uguacuagaa caugguggau guguuacagu aauggcgcaa      720

gacaaaccua cagucgauau agaacuagua acaacuacag uaagcaauau ggcagaaguc      780

cgaucauacu guuacgaagc aaguauaucu gacauggcaa gcgacagucg auguccaaca      840

caaggagaag cauaucuaga uaaacaaucu gacacacaau auguuugcaa aagaaccuua      900

guugauagag gaugggguaa uggguguggc uuauuuggca aggguagucu uguaaccugu      960

gcaaaauucg cauguaguaa aaaaaugaca ggaaaaagca uacaaccaga aaaucuagaa     1020

uauagaauaa uguuaucagu acaugguagu caacauucag gaaugauagu uaaugacacc     1080

ggacacgaaa cugaugaaaa ucgagcaaaa guagaaauaa caccaaauag uccacgagca     1140

gaagcuacau uaggcggauu uggaucguua ggauuagauu gugaaccaag aacuggauua     1200

gauuuuagug aucuauauua ccuaacaaug aauaauaaac acugguuagu acauaaagaa     1260

ugguuucacg acauaccacu acccuggcac gcuggagccg auacuggaac accacauugg     1320

aacaacaaag aagcacuagu agaguuuaaa gaugcacacg caaaaagaca aacaguagua     1380

guauuaggau cacaagaagg cgcaguccau acagcacuag caggagcauu agaagcggag     1440

auggacggag caaaaggaag acuaaguuca ggccauuuaa aaugcaggcu aaaaauggau     1500

aaauuaagau uaaaaggagu aucauauuca cuauguaccg ccgcauuuac auuuacaaaa     1560

auaccagcag aaacuuuaca uggcaccguu acugucgaag uccaauaugc uggaacagau     1620

ggaccaugua agguuccagc ucaaauggca guggauaugc aaacacuaac uccaguaggu     1680

agacuaauaa cagcaaaucc gguaauuacu gaaucaacag aaaacucaaa aaugaugcua     1740

gaauuagauc cuccguucgg agacucguac auaguuauag guguagguga aaaaaaaaua     1800

acacaucauu ggcauagauc aggaucaacc auagggaaag cuuuugaggc uacgguaaga     1860

ggagcaaaac gaauggccgu uuuaggagau acugcauggg auuuuggaag cguaggagga     1920

gcauuaaacu cauuaggaaa aggaauacau caaauauuug gagcagcauu uaaaagucua     1980

uuuggaggaa ugucaugguu uucacaaaua cuaauaggaa cucuguuaau gugguuagga     2040

cuaaauacaa aaaauggauc uauaagccuu augugccuag cucuaggagg aguuuuaaua     2100

uucuuaagua cagcuguauc agcagauguu ggauguucug uagacuucuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4795
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 1 opt

<400>  4795
gggagaaagc uuaccaugag aauuauaaau gcacgaaaag aaaagaaaag aagaggcgcu       60

gauacaagcg ugggaauugu gggauuauua uuaacaacag caauggcagc ggaaguuaca      120

agaagaggaa gugccuauua uauguaucua gauagaaaug acgcaggaga ggcaaucuca      180

uuuccaacua cacuuggaau gaauaaaugu uacauccaaa uaauggacuu aggacauaug      240

ugcgaugcaa cuaugucaua cgaauguccg auguuagaug aaggaguaga gccagacgau      300

guagauugcu gguguaauac aacuucaaca uggguaguau acggcacgug ucaucauaag      360

aaaggcgaag cuagacgauc caggagagca guaacauuac cuucacauag uacaagaaaa      420

uuacagacaa gaucacaaac augguuagaa ucuagagaau auacaaaaca ucuaauuaga      480

guugaaaacu ggauauuuag aaacccagga uucgcacuag cagcugcagc aauagcaugg      540

uuacuaggca gcucuacgag acaaaaagua auauaccuug uuaugauacu ccuaauagca      600

ccagccuauu caauuagaug cauaggagua aguaacagag auuuuguuga aggaaugagc      660

ggagguacau ggguagaugu aguauuagaa cauggaggau gugugacagu aauggcacag      720

gauaaaccaa cuguagacau agaauuagua acaacaacug uaagcaauau ggcagaagua      780

agaaguuacu guuaugaagc cucaaucagc gauauggcaa gugauucaag auguccaaca      840

caaggugagg cuuauuuaga uaaacaaagc gacacacaau auguaugcaa aaggacauua      900

guagacagag gauggggaaa cgguugcggc cuauucggaa aaggaagucu aguuacaugu      960

gcaaaauuug cuuguucuaa gaaaaugaca ggaaaaagca uacagcccga aaaccuagaa     1020

uaucgcauaa uguuaagcgu acauggauca caacauagug gaaugauagu uaaugauaca     1080

ggacacgaaa cagaugaaaa cagagcaaaa guggaaauaa cuccaaauuc acccagagca     1140

gaagcaacac uaggaggauu cggaucacua ggacuagauu gugaaccgag gaccggauug     1200

gauuuuagug auuuguacua uuugacuaug aauaacaaac auuggcuagu acacaaagaa     1260

ugguuucaug auauaccauu accauggcau gcaggagcag auacggguac accacauugg     1320

aauaacaaag aagcauuagu agaauuuaaa gacgcacaug caaaacgaca aacaguagua     1380

guacuuggga gucaggaagg agcaguacau acagcauuag caggagcauu agaagcagaa     1440

auggauggug caaaagguag acuuucaucc ggacaccuga aaugucgacu aaaaauggau     1500

aaauuaagau uaaaaggggu gagcuacagc uuguguacug cagcauuuac uuuuacuaaa     1560

auaccagcug aaacacuuca uggaacagua acuguagaag ugcaguaugc aggaacagau     1620

gguccaugua aaguaccagc acagauggcu guagauaugc aaacucuaac accuguuggc     1680

aggcuaauaa cugcaaaucc aguaauaaca gaaucaacag aaaacucaaa aaugauguug     1740

gaauuagauc caccauuugg agauaguuac auaguaauag guguugggga aaagaaaauu     1800

acucaucauu ggcacagauc aggaucuacg auaggaaaag cauuugaagc uacuguaaga     1860

ggcgcaaaaa ggauggcagu auuaggugau acggcauggg acuuuggauc uguaggugga     1920

gcguugaaua gucuagguaa aggaauacau caaauauuug gagcagcauu uaaaucauua     1980

uuugguggca ugaguugguu uagucagaua cuuauaggaa cucuauuaau gugguuagga     2040

uuaaacacca aaaauggauc aauaucacuu auguguuuag cauuaggggg aguccuaauc     2100

uuccuaucaa cagcaguaag cgcggaugua ggauguucag uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4796
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 1 opt

<400>  4796
gggagaaagc uuaccaugcg uauuauaaau gcaagaaaag aaaagaaaag aagaucagca       60

gauacaucag uugguauugu gggcuuacuu cucacaacag cgauggcagc cgaaguaacu      120

agaagaggau cagcuuacua cauguauuua gacaggaaug acgcugggga agcuauauca      180

uuuccuacaa cauuaggaau gaauaaaugc uauauacaaa uaauggaccu uggacacaug      240

ugugaugcaa ccaugagcua ugaaugucca auguuagacg aaggaguaga gccagaugau      300

guagacuguu ggugcaauac uacuuccacc ugggugguau auggaacaug ccaccauaag      360

aaaggugaag caagaagaag uagacgagca guaacacugc caucacacag uacaagaaaa      420

cuucaaacaa gaagccaaac auggcuagaa aguagagaau auacaaaaca uuuaauaaga      480

guagaaaacu ggauauuuag aaauccugga uuugcuuuag cugcugcagc aauagcaugg      540

cuauugggaa gcaguacaag ccaaaaaguc auauaccuag uaaugauacu ucuuauagca      600

ccagcauauu caauaagaug uauaggagua ucaaauagag auuuugucga aggcaugagu      660

ggaggaacau ggguagaugu uguuuuagaa cauggaggau guguaacagu aauggcacaa      720

gauaaaccaa caguagacau agaauuagua acaacaacag ucucaaacau ggcagaagua      780

agaucauauu guuacgaagc aucaauuuca gauauggcuu cagauucaag auguccuaca      840

caaggagaag ccuaccuaga uaaacaaagu gacacucaau acguaugcaa aagaacucua      900

guagauagag guuggggaaa cggauguggg uuauuuggaa aaggaagccu cguaacaugu      960

gcaaaauucg ccugcaguaa gaaaaugaca ggaaaaagca uacaaccaga aaacuuagaa     1020

uauagaauaa ugcuuagugu acacgguucu caacauucag gaaugauagu aaacgauaca     1080

ggccaugaaa cagaugaaaa cagagcgaaa guagaaauaa caccaaauuc acccagagca     1140

gaagccacac uuggaggauu uggcucauua ggauuagauu gugaaccacg aacaggacua     1200

gacuuuagug aucuauauua cuuaaccaug aauaacaaac acuggcuagu acauaaagag     1260

ugguuucacg auauuccacu cccauggcac gcaggagcag auacuggaac accgcauugg     1320

aauaauaaag aagcccucgu agaauuuaaa gaugcacaug cuaaaaggca aacgguggua     1380

guauuaggaa gucaagaagg agccguacac acagcauugg caggggcacu agaggcugaa     1440

auggacggag caaaaggucg auuaucaagu ggacauuuaa aauguagauu aaaaauggau     1500

aaauuaaggc uaaaaggagu aucguauuca uuaugcacag cagcauuuac auuuacaaaa     1560

auuccggcgg aaacacuaca uggaacagua acaguagaag uacaauaugc gggaacagac     1620

gguccaugua aaguaccugc acaaauggca guagauacac aaacuuugac accaguagga     1680

agacuaauua cagcaaaucc aguaauaacu gaaucaacag aaaauucaaa aaugauguua     1740

gaauuagauc caccuuucgg agauaguuac auaguaauug gaguggggga aaagaaaaua     1800

acccaccacu ggcaccgaag cgguucaacu auaggaaaag cauuugaggc aacaguuaga     1860

ggcgcaaaaa gaauggcggu auuaggagac acagcauggg auuucggauc uguaggaggg     1920

gccuuaaacu cacuaggaaa aggaauacau caaauauuug gagcagcguu uaaaucacua     1980

uuugguggga ugucuugguu uagccaaaua cuuauaggca cgcuacuaau guggcucggu     2040

uuaaauacca aaaauggauc uauaaguuua augugucuag cauuaggagg aguacuaaua     2100

uuuuugagua cagcaguguc agcugaugua ggcuguucag uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4797
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 1 opt

<400>  4797
gggagaaagc uuaccaugag aauuauaaau gcaagaaaag aaaaaaaaag acgaggagca       60

gacacuaaug uagguaucgu aggacuauua uuaacgacag ccauggcagc ugaaguaacc      120

cgaaggggau cggccuauua cauguauuua gauagaaaug augcaggaga agcaauaagu      180

uuuccaacaa cguuagguau gaauaaaugu uacauacaaa uaauggauuu aggucauaug      240

ugugacgcaa caaugaguua cgaaugccca auguuagaug aaggugugga acccgaugac      300

guugauugcu ggugcaauac aaccucaaca uggguugucu augggacaug ucaucauaaa      360

aaaggagaag caagaagaag uagaagggcc guuacucuuc caagccauag cacaagaaaa      420

cuccaaacua gaucacaaac auggcuagaa aguagagaau acaccaaaca cuuaauaaga      480

guagaaaauu ggauauuuag aaauccagga uuugcauuag cagcagcagc uauagcaugg      540

cuauuaggau cgucuacaag ucaaaaagua auauauuuag uuaugauacu acuaauagca      600

ccagcauaca gcauaaggug cauaggcgua ucaaacagag auuuuguuga aggaauguca      660

ggaggaaccu ggguagaugu aguguuagaa cauggagggu gcgugacagu aauggcccaa      720

gauaaaccaa caguugauau agaacuuguc acgacaacag ugucaaacau ggccgaagua      780

agauccuauu guuacgaagc aucaauaucg gauauggcau ccgauagcag auguccuaca      840

caaggagaag cauauuuaga caaacaaagc gauacacaau auguauguaa acgaacauua      900

guugacagag ggugggguaa ugggugugga cuauucggua aagguaguuu agucacuugc      960

gcaaaauuug cauguucuaa gaaaaugaca ggaaaaucaa uacaaccgga aaaccuagaa     1020

uacagaauaa ugcuaucagu gcauggauca caacauagcg gaaugauagu aaaugacacu     1080

ggucacgaga cagaugaaaa uagagcaaaa guagaaauaa caccaaauuc accaagagcg     1140

gaagcaacau uaggagguuu uggaucauug ggauuagauu gcgaaccaag aacaggauua     1200

gauuuuucag aucuuuauua ucugacaaug aauaauaaac auuggcuagu gcacaaagaa     1260

ugguuccaug auauaccccu accauggcau gcaggugccg auaccggaac accacauugg     1320

aauaauaaag aagcauuagu ugaauuuaaa gaugcucacg ccaaaagaca aacaguagua     1380

guauuaggcu cacaagaagg ggcaguacau accgcauugg cuggagcguu agaagccgaa     1440

auggacggug ccaaaggaag acucucaagu ggucauuuga aaugcagacu aaaaauggau     1500

aaauuaagau uaaaaggagu aucauauagu uuaugcacag cugcauuuac auuuacaaaa     1560

auaccagcag aaacucuaca uggaacaguu acaguugagg uucaauaugc aggaacagau     1620

ggaccaugca aagucccugc acaaauggca guagacaugc aaacacugac accuguagga     1680

agauuaauaa cugcaaaucc gguaauaaca gaaaguaccg aaaacucgaa aaugauguua     1740

gaacuugauc cuccguucgg agacagcuac auuguaauag gaguaggaga aaaaaaaaua     1800

acgcaucacu ggcauagauc aggaucgacc aucggaaaag cauuugaggc cacugugagg     1860

ggagcaagaa gaauggcagu auuaggcgau acagcuuggg auuuugguuc agucggagga     1920

gcauugaaua guuugggaaa aggaauacau caaauauuug gagcagcauu uaaaucauua     1980

uuugguggua ugagcugguu cucccagaua uuaauaggaa cacuauuaau gugguuaggu     2040

uuaaauacua aaaauggaag uauaucauua auguguuuag cuuuaggagg cguacuaaua     2100

uuuuuaucaa cggcuguuuc agcagaugug ggaugcucag uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4798
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 1 opt

<400>  4798
gggagaaagc uuaccaugag gauaauuaau gcuagaaaag aaaagaaaag aagaggagca       60

gaaacaagug uaggaaucgu ugguuuauua uuaacaacug cuauggcagc cgaaguaaca      120

aggagaggga gugccuauua cauguauuua gacagaaaug acgcaggaga agcaauuucc      180

uucccaacua cuuuagguau gaacaaaugu uauauacaaa uaauggauuu gggacauaug      240

ugcgaugcga caaugaguua ugaaugucca augcuagacg aaggagucga accugaugac      300

guagacuguu gguguaacac cacaaguaca uggguaguau auggaacgug ccaucauaag      360

aaaggagaag cgagaagaag uaggagagcc guaacauuac caagucacag cacaagaaaa      420

uuacaaacaa gaagucaaac augguuagaa ucaagagaau auacaaaaca ccuaauaagg      480

guagaaaacu ggauauuuag aaauccagga uuugcauugg cagcagcagc cauagcaugg      540

uuguuaggau caaguacaag ucaaaaagua auauauuuag uaaugauauu auuaauagca      600

ccagcauauu caauaagaug cauaggugua ucaaauagag acuuuguaga agguaugagu      660

ggaggaacuu ggguagaugu aguuuuagaa cacggaggau gcguaacagu aauggcucaa      720

gacaaaccaa caguagauau agaauuaguc acuacaacag uaucaaauau ggcagaaguc      780

agaagcuauu guuacgaagc aagcauuuca gacauggcau cugauucacg uuguccuacg      840

caaggagaag ccuauuuaga uaaacaaucc gacacacaau auguauguaa aagaacccua      900

guagacagag guugggguaa uggauguggc cuauucggga aaggaaguuu agucacaugu      960

gcuaaauuug caugcaguaa gaaaaugaca ggaaaaucaa uacaaccgga aaaucuagaa     1020

uacagaauaa ugcuaagugu acauggaagu caacauuccg gcaugauagu gaaugauacu     1080

gggcaugaaa ccgaugaaaa cagagcaaaa guagaaauaa caccaaauuc uccgcgugcu     1140

gaagcuacac uagggggauu uggaucauua ggucuugacu gugaaccaag gacugguuua     1200

gauuucucgg aucuauauua ucuaacuaug aauaacaaac auuggcuugu acauaaagaa     1260

ugguuucacg auauaccacu accauggcau gcaggagcag auacagggac uccucacugg     1320

aacaauaaag aagcauuagu ugaguuuaaa gaugcacaug caaaaagaca aacuguaguu     1380

guauuaggcu cacaagaagg ugcaguacac acagcucuag caggggcucu agaggcggaa     1440

auggauggug caaaaggaag acuuucaagu ggacacuuaa aaugcagacu aaaaauggau     1500

aaauugagau uaaaaggagu aaguuauucu uuauguacag cagcauucac auuuacaaaa     1560

auaccagcag aaacauuaca uggaacagua acaguugaag uacaauacgc gggaacagau     1620

gggccaugca aagucccagc gcaaauggcu guagacaugc aaacgcugac acccguaggg     1680

cgacuaauua cugcaaaucc aguaauaacu gaaaguacag aaaacaguaa aaugaugcug     1740

gaauuagauc ccccguuugg agacagcuau auaguaauag gaguagguga aaagaaaauc     1800

acacaucauu ggcauagauc agguucaaca auaggaaaag ccuuugaagc aacaguuaga     1860

ggagccaaac ggauggcagu gcuuggagau acggcuuggg acuucggauc aguaggaggc     1920

gcauuaaaca guuuagggaa aggaauacac caaauuuucg gugcagcuuu uaaaaguuua     1980

uuuggaggaa ugucaugguu uucucaaaua uuaauaggaa cuuugcuaau guggcuagga     2040

uuaaacacca aaaauggauc aauaucacug augugucuag cucuaggugg uguauuaaua     2100

uuucuaucaa cagcaguuuc agcagauguu ggaugcucag uugauuuuuc gaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4799
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 1 opt

<400>  4799
gggagaaagc uuaccaugag aauaauaaau gccagaaaag aaaagaaaag aagaggugca       60

gauacauccg uaggaauagu aggauuacuu uuaacaacag caauggcagc agaaguaaca      120

agaagggguu cagcauauua uauguaccua gauaggaaug acgcgggaga agcuauauca      180

uuucccacaa cccuaggaau gaauaaaugc uauauacaga ucauggaccu uggacauaug      240

ugugaugcaa caaugucaua cgaaugucca auguuagaug aaggaguaga accggaugac      300

gucgauugcu ggugcaacac uacgaguacc uggguagucu auggaacaug ucaucauaaa      360

aagggagaag caagacgaag uaggagagca gucacuuugc caagucacag uacuagaaaa      420

cugcagacaa gaagucaaac augguuagaa ucaagagaau auacuaagca ccuaauaaga      480

guugagaauu ggauauuuag aaacccaggu uuugcucuug cagcugcggc uauagcgugg      540

cuguuaggau caucaacaag ucaaaaaguu auauacuuag ugaugauauu auuaauugcu      600

ccugcauauu cacuaagaug uauuggagua aguaauagag auuuuguaga agguaugagu      660

ggaggcacgu ggguugaugu aguacuagaa cauggcggau guguuacagu aauggcacaa      720

gauaaaccua caguagauau agaauuagua acuacuacag uaaguaauau ggcagaaguu      780

agaaguuacu guuaugaagc aaguauauca gauauggcaa gugauucaag augucccacg      840

caaggagagg cuuaucuaga uaaacaauca gauacacaau acgucuguaa acgaaccuua      900

guagaucgag guuggggaaa ugggugcggu uuauucggaa aaggaucauu aguaacuugu      960

gcaaaauuug cauguucaaa gaaaaugaca ggaaaaagca uccaaccaga aaacuuagaa     1020

uauagaauaa uguuaagugu acaugguucu caacauucag gaaugauagu aaacgacaca     1080

ggccaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccaaauuc accaagagcu     1140

gaagcgacuu uaggaggauu ugguagcuua gguuuagauu gugaacccag aacaggauug     1200

gauuucagug auuuauauua ucuaacgaug aauaacaaac auugguuagu acauaaagaa     1260

ugguuccaug auauaccauu gccuuggcau gcuggagcag auacaggaac gccacacugg     1320

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg cgaaaagaca gacaguaguc     1380

guacuaggau cacaagaagg agcaguacau acagcauugg ccggagcuuu agaagccgaa     1440

auggauggag caaaaggcag auuaagcagu ggccacuuaa aaugucgauu aaaaauggau     1500

aaauuaagau uaaaaggagu caguuacagc uuauguacag cugcauucac auuuaccaaa     1560

aucccagcag aaacauuaca ugguacugug acaguagaag uacaauacgc uggaacagau     1620

ggcccuugua aaguaccagc acaaauggca guagauaugc aaacauuaac accgguagga     1680

agguuaauua cugcaaaucc aguaauaacu gaaucaacag aaaauaguaa aaugaugcua     1740

gaauuagacc cacccuuugg ggauaguuau auagugauag gaguaggaga aaagaaaaua     1800

acacaccacu ggcaucguag cggaaguaca auaggaaaag cauuugaagc uacgguaaga     1860

ggggcaaaac gaauggcugu gcuaggggac acagccuggg auuucggauc agugggaggu     1920

gcauuaaauu cauuaggaaa agggauacau caaauauucg gagcugcguu uaaaucauua     1980

uucggaggaa ugaguugguu uucucaaaua uuaauuggaa cgcuucuaau guggcuagga     2040

cuaaauacua aaaacgguuc aauaucucug auguguuuag cauugggagg aguacuaaua     2100

uuccuuagua cagcaguaag ugcggaugua ggcugcucag uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4800
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 1 opt

<400>  4800
gggagaaagc uuaccaugag aauuauaaac gcaagaaaag aaaaaaagag aagaggagcu       60

gacacuaaug uaggaauagu aggacuguua uuaacaacag cgauggcagc agaaguaacg      120

agaagaggau cugcauacua uauguauuua gauaggaaug augcaggaga agccauauca      180

uuuccgacaa cauugggaau gaauaaaugu uacauacaaa uaauggaucu aggacauaug      240

ugugacgcaa caaugaguua cgaguguccc auguuagaug aagggguuga gccagacgau      300

guggauuguu gguguaacac aacaaguacu uggguuguau auggaacuug ccaccauaaa      360

aaaggugaag cuagaagauc acguagggcg guaacacuac caucucacag uacccguaaa      420

cuacaaacga gaagccagac augguuagaa agcagagaau auacaaaaca uuuaauaaga      480

gucgaaaauu ggauguuuag aaauccaggc uuugcauuag cagcagccgc uauagcaugg      540

cuucuuggau caaguacauc acaaaaaguu auauauuuag uaaugauauu guuaauagcc      600

ccagcuuacu caauaagaug uauuggagua agcaacagag auuuuguaga aggaaugagu      660

ggaggaacau ggguugaugu aguacuagag cauggugggu gcguaacagu cauggcgcaa      720

gacaaaccua caguagauau agaauuagua acaacaacag uaaguaauau ggcagaagug      780

cgaucauauu guuacgaagc aagcauuucu gauauggcau cagauagucg uuguccaaca      840

caaggagaag cauaccuaga uaaacaauca gauacccaau acguauguaa aagaacuuua      900

guagaucgag guugggguaa uggaugugga cuauuuggua aaggaagccu aguuacuugu      960

gcuaaguuug caugcaguaa aaaaaugaca gguaaaucaa uacaaccaga aaaucuagag     1020

uauagaauaa uguuaagugu acauggaagu caacauagug guaugauugu aaaugauaca     1080

ggacacgaaa cagacgaaaa ccgagcuaaa guagaaauca cgccaaauag ucccagggca     1140

gaagcaacau uaggaggauu uggcucguua ggauuagacu gugaaccacg aacugggcua     1200

gauuuuagcg auuuauauua ucuaacaaug aacaacaaac acugguuagu acauaaagaa     1260

ugguuucaug auaucccacu accuuggcau gccggagcag acacaggaac accacacugg     1320

aauaauaaag aagcacuagu agaguuuaaa gacgcacaug cgaaaaggca aacaguagua     1380

guacuaggau cacaagaagg agcgguacau acggcacuag cuggagcccu agaagcagag     1440

auggacggcg cuaaaggaag auugagcagu ggacaccuaa aaugucguuu gaaaauggau     1500

aaauuaagac ucaaaggagu aaguuauucg uuaugcacag cugcguuuac auuuaccaaa     1560

auaccagcag aaacgcuaca uggaaccgua acgguggaag uucaauaugc aggaacagau     1620

ggaccaugca aaguaccagc ucagauggcg guagacaugc aaacauuaac accaguaggg     1680

agguuaauaa cagcaaaccc ugugauaacu gaaaguacag aaaauaguaa gaugaugcua     1740

gaacuagauc caccauucgg agauucauau auaguaauag gaguagggga aaaaaaaaua     1800

acucaucauu ggcauagaag ugguaguaca aucggaaaag cauuugaagc aacaguacgc     1860

ggggcaaaaa gaauggcagu acuuggagau acagcauggg auuuuggaag uguaggaggu     1920

gcguuaaaua guuuaggaaa aggaauacau caaauauucg gggcagcauu caaaaguuua     1980

uuuggaggaa ugaguugguu cagucaaaua uuaauaggaa cauuguuaau guggcuaggu     2040

cuuaauacaa aaaaugguuc aauaaguuua augugucuug cauuaggagg agucuuaaua     2100

uuuuuaagca cagcaguaag ugcggacguu ggguguucag uugacuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4801
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 1 opt

<400>  4801
gggagaaagc uuaccaugag aauaauaaau gcacgcaaag agaaaaaaag aagaggagca       60

gauacaagug uaggaauagu ugguuuauua cuaacaacag caauggcagc ugaagucaca      120

agaagaggau cagcauauua uauguaccua gacagaaacg augcuggaga agcgauauca      180

uuuccuacga cacuugguau gaauaaaugc uauauucaaa uaauggacuu gggacauaug      240

ugugaugcaa ccaugucaua cgaaugcccu auguuagaug aggguguaga accugacgau      300

guagauugcu gguguaacac aacuaguacu uggguaguuu auggaacaug ucaccacaaa      360

aagggagaag caagaagauc aagaagggcc guaacucucc caucacacag uacaagaaaa      420

uuacaaacaa gaucacaaac gugguuagaa ucaagagaau auacaaaaca cuuaauaaga      480

guugaaaauu ggauacuacg uaauccagga uucgcacucg cagcugcggc uauagcaugg      540

cucuuagguu cgaguaccuc ucagaaggua auauacuuag uaaugauauu auuaauagca      600

ccagcuuauu cuauaagaug uauaggaguc aguaauagag auuuuguuga aggaauguca      660

ggaggaacuu ggguagacgu gguacuggaa cacggaggau gcgugaccgu uauggcgcaa      720

gauaaaccaa cgguagauau agaauuagua acaaccacag uuaguaauau ggccgaggua      780

aggucuuauu guuaugaagc cucuauaucu gacauggcau cagacucaag augcccuaca      840

caaggagaag cauauuuaga uaaacaauca gauacacaau auguauguaa acgaacacua      900

guagauaggg gcugggguaa uggaugcgga cuauucggaa aaggaaguuu agucacaugu      960

gcaaaauucg cauguucuaa aaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa     1020

uaucgaauua uguuaucagu acauggauca caacauucug gaaugauagu aaacgacaca     1080

ggccacgaaa cagacgaaaa uagagcaaaa guugaaauaa caccaaauag uccucgagcc     1140

gaagcaacac uuggagguuu uggaagucua ggauuagauu gugaaccaag aacaggauua     1200

gacuucucgg aucuauauua ucugaccaug aauaauaaac auugguuagu gcacaaagaa     1260

ugguuucaug auauaccguu accauggcau gccggagcag auacaggcac accacauugg     1320

aauaauaaag aagcacuagu agaauucaaa gaugcucacg cuaaaagaca aacaguggua     1380

guauuaggua gccaagaagg agcaguacac acagcccuag caggugcucu ugaagcagaa     1440

auggauggag caaagggaag auuaaguucc ggacaccuga aaugcagacu aaaaauggau     1500

aaauuaagac uaaaaggagu cucauauuca uugugcacag cagcauuuac uuuuacaaaa     1560

auuccagcag aaacguugca cggaacagua acuguagaag uacaauacgc aggaacagau     1620

ggaccgugua aaguaccagc acaaauggca guugauaugc aaaccuuaac accaguugga     1680

agauuaauaa cugcaaaccc aguaauaaca gaaaguacgg aaaauucaaa aaugauguua     1740

gaauuagauc cuccauucgg agauucauac aucguaauag gaauaggaga aaaaaaaaua     1800

acacaucauu ggcacagauc gggaucaaca auagguaaag cauuugaagc uacaguaaga     1860

ggagcaaaac ggauggcagu auuaggagac acugccuggg auuuuggauc aguaggggga     1920

gcauuaaauu cacuaggaaa aggcauccau caaauauuug gugcugcuuu uaaaucccua     1980

uucggaggaa ugagcugguu cucacaaaua cuuauuggaa cucugcuaau guggcuagga     2040

uuaaauacaa aaaauggaag cauaagcuua augugucuag cucuaggagg cguucuaauu     2100

uuuuuaagua cagccguauc agcugacgua ggauguucag ucgauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4802
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 1 opt

<400>  4802
gggagaaagc uuaccaugag aauaauuaac gcuagaaaag aaaagaaaag aagaggagcu       60

gacacaagcg ugggaauagu aggacucuug cuaacaacag caauggcugc agaaguaaca      120

agaagaggaa gugcauauua uauguauuug gaucgaaaug acgcaggaga agcaauauca      180

uuuccaacaa cacucggaau gaauaaaugc uauauacaaa uaauggaucu cggacauaug      240

ugcgacgcca caaugucuua ugaauguccu augcuagaug aaggaguaga accagacgau      300

gucgauuguu gguguaauac aacaagcaca ugggugguau acggaacaug ucaucauaag      360

aaaggugaag caaggagaag uagacgggca guaacacuac caucacauag uacgcgaaaa      420

cuacaaacaa gaucacaaac uuggcuagaa ucaagagaau acacaaaaca ucuaauuaga      480

guagaaaacu ggauauuuag aaacccaggc uucgcacugg cagcagcagc aauagcaugg      540

uugcuagggu caagcacauc acaaaaaguu auauauuuag uaaugauauu auugauagcu      600

ccagcauaca guauaagaug uauaggagug ucaaaucgag auuuuguuga aggaauguca      660

ggaggcacuu ggguagaugu aguauuagaa cauggugguu gcguaacagu aauggcucaa      720

gauaaaccaa caguagauau cgaauuagua ucaacaacag uuucaaacau ggcagaagua      780

agaucuuauu guuacgaagc aaccauaagc gauauagcaa gugauucaag guguccuaca      840

caaggagaag cauaucuaga caaacaauca gauacacaau acguaugcaa aagaacauua      900

guugauagag gauggggaaa uggaugcggg uuauuuggaa aagggaguuu aguaacuugu      960

gcaaaauuug cguguucaaa gaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa     1020

uaucgaauua uguugucagu acauggauca caacauagcg ggaugauagu caaugauaca     1080

ggacaugaaa cugacgaaaa uagagcaaaa guagaaauaa caccaaauag cccuagagcc     1140

gaagcuacau uaggaggauu ugguucacuc ggacuagacu gugaaccaag gacuggucuu     1200

gauuuuucag auuuauauua cuuaacaaug aauaauaaac acugguuagu ccacaaagaa     1260

ugguuucaug acauaccauu gccuuggcau gcaggagcag auaccggaac accacacugg     1320

aauaauaaag aagcauuggu agaauuuaaa gacgcucaug caaaaaggca aacagcuguu     1380

guacucggau cucaagaagg agcaguucac acagcuuuag cuggagcacu ugaagcagaa     1440

auggauggag caaaaggaag acuaucuucg ggccauuuaa aaugcagacu aaaaauggau     1500

aaauugcgau uaaaaggggu uaguuacagu uuauguacag cggcuuuuac uuuuacaaaa     1560

auaccagcag aaacacuaca uggaacagua acaguagaag uacaauaugc ggguacagau     1620

ggcccaugca aaguaccagc acaaauggca guagauaugc aaacacuaac gccaguagga     1680

aggcugauua cagcaaaucc aguaauaaca gaaucuacug aaaacucaaa aaugaugcuc     1740

gaacuagauc caccauuugg ggauucuuau aucguuauag gcguaggcga aaagaaaauu     1800

acacaccauu ggcacagauc aggaucaaca auaggaaaag ccuuugaagc uacaguaaga     1860

ggugcaaaaa gaauggcugu ucuuggagac acagcauggg auuuugguag uguaggggga     1920

gcauugaacu cacuagggaa aggcauacau caaauuuuug gugcagcauu uaaaucauua     1980

uucggaggaa ugagcugguu uagccaaauu cuaauuggaa cuuuacugau guggcuaggc     2040

cuuaauacua aaaauggaag uauaucacuu augugcuuag cauuaggagg aguacugaua     2100

uuuuuaucaa cagccguuuc agcagacguu gguugcagug uugauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4803
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 1 opt

<400>  4803
gggagaaagc uuaccaugag aauaauaaac gcucguaaag aaaaaaagag aagaggagca       60

gacacaagug uaggcauagu aggauugcuu uugacuacag caauggcagc agagguaaca      120

aggagagggu cagcuuauua uauguacuua gacagaaacg acgcagguga agccauaucc      180

uuuccgacaa cacuagguau gaauaaaugc uacauacaaa ucauggauuu aggacauaug      240

ugcgaugcaa caaugagcua cgaaugucca auguuagaug aaggaguaga accagaugau      300

guugauuguu gguguaacac aacuagcaca uggguagucu auggcacaug ucaccacaaa      360

aaaggcgaag cuagaagauc cagacgagcc guaacauuac cuagucauag cacgagaaag      420

cuacaaacaa gaagccaaac augguuagaa aguagagaau auacaaaaca ucuaaucaga      480

guggaaaacu ggauauucag aaaccccggu uuugcauuag cugcagcagc cauagcaugg      540

cuauuaggaa guucaacgag ccaaaaagua auauauuuag uaaugauauu acugaucgcu      600

ccagcauauu caauacgaug uauaggggua agcaauagag acuucgucga aggaauguca      660

ggaggaacau ggguagaugu ugugcucgaa cacggugguu gugucacagu aauggcacaa      720

gauaaaccaa ccgucgauau agaacuagua acaacaacag uuucaaauau ggcagaagua      780

agaucuuacu guuaugaggc aaguauaagu gauauggcgu cagauucaag augcccaacu      840

caaggagaag cauauuuaga uaaacaaagu gauacacaau acguauguaa aagaacuuua      900

guagauagag gauggggaaa uggcuguggu cuauuuggca aaggaagucu aguaacuugu      960

gcaaaauucg caugcagcaa aaaaaugaca ggaaaaagca uacaacccga aaacuuagaa     1020

uaucgaauaa ugcugucagu ccauggaagu caacacagug guaugauagu uaaugauacu     1080

ggacaugaaa cagacgaaaa uagagcgaaa guagagauaa cuccaaauuc gcccagagca     1140

gaagcuaccc uaggagguuu uggaucacuc gguuuggauu gcgaaccucg aacagguuua     1200

gacuuuagcg acuuauauua ccuaacaaug aacaauaaac auuggcuagu acacaaagaa     1260

ugguuucaug auauaccuuu accauggcau gcuggcgcag auacaggaac accgcauugg     1320

aauaauaaag aagcacuagu agaauuuaaa gaugcacaug ccaaaagaca aacugucguu     1380

guauuaggua cacaagaagg agcuguacau acagcauuag ccggagcauu agaagcagag     1440

auggacggug caaaaggccg auuaaguuca gggcaucuaa aauguagauu gaaaauggac     1500

aaauuacgac uaaaaggagu uucauauuca uuauguaccg cagcauucac uuuuacaaaa     1560

auaccugcag agacauuaca ugguacagua acaguagaag uacaauaugc aggaacagac     1620

ggaccaugua aaguaccugc ucaaauggca guagauaugc agacucuaac accagucggc     1680

agauuaauaa cagcaaaucc cguaauaaca gaaucgacgg aaaauucuaa aaugauguua     1740

gaacucgauc cuccauuugg agauaguuac auaguaauug ggguaggaga aaaaaaaauc     1800

acucaucauu ggcauagaag uggaucgacg auaggaaaag cauucgaagc aacaguacga     1860

ggagcuaaaa gaauggcugu uuuaggugac acugcauggg acuuuggauc aguuggagga     1920

gcguuaaaua gcuuaggaaa gggaauacau cagauauuug gagcagcguu caaaagucuc     1980

uucgguggaa ugucaugguu cagucaaaua cugauaggua ccuuauuaau guggcuagga     2040

uuaaauacaa aaaacgguuc cauuagucuu auguguuuag cauuaggcgg aguauuaaua     2100

uucuuaucaa cagcaguauc ggcagacgua ggauguagug uagauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4804
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 1 opt

<400>  4804
gggagaaagc uuaccaugag aauaauaaau gcccguaaag aaaagaaaag aagaggugca       60

gauacaucag uggguaucgu agguuuauua uuaacaacag cgauggcagc ggaaguaaca      120

agaagaggcu cagcauauua uauguaccua gacagaaacg augcgggaga agcaauaucu      180

uuuccuacaa cacuuggaau gaauaaaugc uauauacaaa uuauggacuu aggacacaug      240

ugugaugcua caaugagcua cgaaugucca auguuagaug aaggaguaga accagaugac      300

gucgauugcu ggugcaacac uacgagcaca uggguuguuu acgguacaug ccaccauaag      360

aaaggagaag cacgaagauc aagaagggcu gucacguuac cgucacauuc aacaagaaaa      420

uugcaaacaa gaucacaaac augguuagag ucaagagaau auacaaaaca uuuaauuaga      480

guagaaaauu ggauuuuuag aaauccaggu uuugcacuug cagcagcugc uauagcaugg      540

uuauuaggau caagcacauc acaaaaaguc auauauuuag ugaugauuuu acuaauugca      600

cccgcauaca gcauaagaug uauaggagua ucaaauagag acuucguaga aggaaugucg      660

ggaggaacuu ggguagaugu aguauuagaa caugggggau guguaacagu aauggcucag      720

gauaaaccaa cuguugauau ugaauuggua acaacaacag ugaguaauau ggcugaagua      780

agaaguuauu guuacgaagc aaguauaagu gauauggcaa gugauucaag guguccuacu      840

caaggagagg cauacuuaga caaacaaucu gacacucaau auguauguaa acgaacauua      900

gucgaucgag guugggguaa uggauguggu cuauuuggaa aaggaucgcu aguaacaugu      960

gccaaauuug cauguaguaa gaaaaugaca ggaaaaagua uucaaccaga gaacuuagaa     1020

uaucgaauua uguuaucagu ucauggaagu caacauagug gaaugauagu aaaugacacc     1080

ggucaugaaa cggaugaaaa uagagcaaaa guugagauaa caccaaauag uccuagagca     1140

gaagcaacac uaggagguuu cggaagucua gguuuagauu gugaaccacg aacaggauua     1200

gacuuuagcg aucuauacua cuuaacuaug aacaauaaac acugguuggu acacaaagaa     1260

ugguuucaug acauaccacu accguggcac gcuggagcug auacuggaac accacauugg     1320

aauaauaaag aagcauuggu agaguuuaaa gaugcacacg caaaaagaca aacaguagua     1380

guauuaggaa gccaagaagg agcaguacau accgcauugg cuggugcauu agaggcagaa     1440

auggauggug cuaaagguag guuaaguuca ggccauuuaa aauguagauu aaaaauggau     1500

aaauuaagac uaaaaggugu cucauacagc uugugcacag cagcauucac auuuacaaaa     1560

aucccugcag aaacauugca uggaacggua accguagaau uacaauaugc aggcacagau     1620

ggaccuugua aaguaccagc ucaaauggca guagauaugc aaacuuuaac uccaguagga     1680

agauuaauaa cugcaaaucc agugauaacg gaaaguacug aaaacaguaa aaugauguua     1740

gaacuugauc cgccauuugg agacagcuac auagugauag gugugggaga aaagaaaaua     1800

acccaucauu ggcaccgauc aggaucaaca auaggaaagg cuuucgaagc aacaguuaga     1860

ggcgcaaaac gcauggcugu auugggagau acugcauggg auuuuggaag uguagguggg     1920

gcauuaaaca guuuaggaaa aggaauacau caaauuuucg gcgcagcauu uaaaucauua     1980

uucggaggaa ugagcugguu uucacaaaua cuuauaggaa cacuacuaau gugguuaggu     2040

uuaaauacua aaaacggaag uauuucauua auguguuuag cacucggagg uguauuaaua     2100

uuccuaucaa cagcaguauc agcagacgua ggaugcagcg uagacuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4805
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 1 opt

<400>  4805
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaagaaaag aagaggagca       60

gaaacaagug uaggaaucgu uggauuauua uuaacaacag caauggcagc agaaguaacg      120

agacggggau cagcuuauua cauguauuug gauagaaaug augcaggaga agcuauuagc      180

uuuccaacaa cacuggguau gaauaaaugu uauauacaaa uaauggacuu gggucauaug      240

ugcgacgcaa caaugucaua ugaauguccc auguuagaug aaggaguaga accagacgau      300

guagauuguu gguguaauac aacaaguaca uggguaguau augguacgug ccaucauaaa      360

aagggagaag caaggagauc aagaagggca guaacauuac cuagccauag cacucgaaaa      420

uuacaaacaa gaagucagac gugguuggaa ucaagagagu auacaaaaca cuuaauacgg      480

auagaaaauu ggauauucag aaacccaggu uucgcucugg cugccgcagc aauugccugg      540

cuauuagggu caagcaccuc acagaaagug auuuacuuag uaaugauacu auuaauagcu      600

ccugcauaca gcauuagaug uauaggcguu ucaaaccgag acuuugucga aggaaugucg      660

ggagguacau gggucgaugu aguauuagag cacggaggau gugugacagu aauggcacaa      720

gacaaaccaa cagucgacau agagcuagua acaacaacug uaaguaauau ggcagaaguu      780

agaucauauu guuaugaagc caguauaagu gauauggcua gugauuccag auguccaaca      840

caaggagaag cauaucuuga uaaacaaagc gauacacaau auguuuguaa aagaacuuua      900

guagauaggg gaugggguaa cgggugugga cuauuuggaa aaggaucuuu aguuacaugu      960

gcaaaauuug cauguaguaa gaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa     1020

uauagaauua uguugucagu acaugguuca caacauagug guaugauagu aaaugacacc     1080

gggcaugaaa cagacgaaaa uagagcaaaa guagaaauaa ccccaaauag cccgagagca     1140

gaggcaacau uggguggauu uggaagccuu ggacuagauu gugaaccaag aacagggcua     1200

gauuucagug accuguacua uuuaacaaug aacaacaaac auugguuagu ccauaaagag     1260

ugguuccaug auauccccuu accauggcac gcaggagcag acacgggaac accacacugg     1320

aacaauaaag aagcauuagu cgaauuuaaa gaugcacaug caaaaagaca aaccguagug     1380

guauuaggau cacaagaagg ugcaguucau acagcuuuag cuggagcacu ugaagcagaa     1440

auggauggcg caaaaggaag auuaucaagu ggacauuuaa aauguagauu aaaaauggau     1500

aaauugagac uaaaaggugu aucauacagu uuauguacag cagcauuuac uuuuacaaaa     1560

auaccugcag aaacauuaca cgguacagua acaguagaag uacaauaugc aggaacagac     1620

ggaccaugca aagucccggc acaaauggcu guagauaugc aaacacuaac accaguggga     1680

agauuaauua cagcaaaucc aguaauaaca gaaucaacug aaaauucgaa aaugaugcua     1740

gaauuggauc caccauuugg agacaguuau auaguaauug guguuggcga aaagaaaaua     1800

acacaucauu ggcauagauc aggaucaacu auaggaaaag cauuugaggc uacagugcga     1860

ggagcuaaac gaauggcagu guuaggugau acggcauggg auuuuggaag uguaggcggu     1920

gcccuaaauu cacuuggaaa aggcauacac caaaucuuug gagcagcauu caaaucgcua     1980

uuugguggaa ugaguugguu uagucaaaua uuaaucggga cuuuauuaau gugguuagga     2040

uuaaauacua aaaauggaag cauaucacua auguguuuag cguuaggagg aguauuaauu     2100

uucuuaagca cagccguauc agcagaugug ggaugcagug uagauuucuc gaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4806
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 1 opt

<400>  4806
gggagaaagc uuaccaugag aaucauaaau gcuagaaaag aaaaaaaaag acggggagca       60

gauacaucag uaggaauagu gggauuauua cuaacaacug caauggccgc agaaguuaca      120

cgaagaggga gugcauauua uauguaucua gaccgaaaug augcaggaga agcaauaagc      180

uuuccaacaa cccuuggaau gaauaaaugu uauauacaaa uaauggauuu aggacauaug      240

ugugacgcaa caaugucaua ugaaugcccg auguuagacg aagggguaga accagacgau      300

guagacugcu gguguaauac aacaucaaca uggguuguau acggaaccug ccaucauaaa      360

aaaggagaag caagaagaag uagaagggcg guaaccuuac cuucucauuc aacuagaaaa      420

cuucaaacua gaucacaaac uugguuagaa aguagggaau auacaaaaca uuuaauaaga      480

guagagaauu ggauuuuuag aaauccagga uuugcauuag cagcagcagc aauagcaugg      540

uuacucgguu caucaacaag ucaaaaagua auuuacuuag uaaugauuuu acuaauugca      600

cccgccuacu caauuagaug uauaggagua ucaaauagag auuucguaga aggaaugagu      660

ggaggcacuu ggguugaugu aguauuagaa caugguggcu guguaacagu cauggcacaa      720

gauaagccaa caguagauau agaacuagua acaaccaccg uaucaaacau ggccgaagua      780

cgaucguacu guuaugaagc aucaauuagu gacauggcau cagauaguag augucccaca      840

caaggagaag cauaucuaga uaaacaaagu gauacucaau auguauguaa aagaacauua      900

guagaucgag guugggguaa uggaugugga cuauuuggaa aaggaucucu agucaccugu      960

gcaaaauuug cuuguucaaa aaaaaugaca ggaaaaagca uucaaccaga aaauuuagaa     1020

uauagaauua uguuaucagu acauggaagc caacauucug gaaugauagu aaaugauacu     1080

ggacacgaaa cagaugagaa uagggcaaaa guugaaauaa caccaaauuc accuagagca     1140

gaagcaacau uaggcgguuu ugguagcuug gguuuggauu gugaaccuag aacaggacua     1200

gauuuuagug auuuauauua ucuaacaaug aauaauaaac auuggcuggu acauaaagaa     1260

ugguuucaug auauaccauu accuuggcau gcaggagccg auaccgguac gccucauugg     1320

aacaauaaag aagcacuugu agaauucaaa gaugcacacg caaaacguca aacaguagua     1380

guauuaggau cacaagaagg ggcaguacau acggcguuag caggcgcauu agaagcagaa     1440

auggauggag cuaaaggucg ucuuucaagu ggacauuuaa aauguagguu aaaaauggau     1500

aaacuacgau uaaaaggcgu aucuuauuca uuauguacag cagcauuuac uuuuaccaaa     1560

auaccagcag agacccuaca uggaacaguc acgguagaag uacaauaugc ugguacugau     1620

ggaccaugua aaguaccugc ucaaauggcc guagauaugc aaacauuaac accaguggga     1680

agacuaauaa cagcaaaucc gguuauuaca gaaagcauag aaaauucaaa aaugauguua     1740

gaacuagauc cacccuucgg ugauucauau auaguuauag gcguagggga gaaaaaaaua     1800

acgcaucacu ggcaucgauc aggaaguaca auaggcaaag cauuugaagc uacuguacgu     1860

ggagcaaaac gaauggccgu ucucggggac acugcuuggg acuucggcuc aguaggaggg     1920

gcgcuaaaua gucucggaaa aggcauacac caaauuuuug gagcagcuuu caaaucauua     1980

uucggaggaa ugucgugguu cagucaaaua uuaaucggaa cacuacuaau guggcuagga     2040

cucaauacca aaaaugggag uauaaguuua augugucuag cauuaggagg uguuuuaaua     2100

uuuuuaucaa cugcuguuuc agcagacgua ggaugcucag uugauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4807
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 1 opt

<400>  4807
gggagaaagc uuaccaugag aauaauaaac gcaaggaaag aaaagaaaag aagaggagcu       60

gauacaucug uaggaauugu aggguuacua uuaacaacag caauggcugc ggagguaaca      120

aggaggggcu cagcauacua uauguaucua gaccgaaaug augcaggaga agcaauaagu      180

uucccaacaa cauugggaau gaauaaaugu uacauacaaa uaauggauuu aggccauaug      240

ugcgaugcaa caaugaguua ugaaugcccg augcuagaug aaggaguuga acccgaugau      300

gucgauuguu gguguaauac aacaaguacu uggguaguau acggaacaug ucaucauaag      360

aaaggugaag cuagaagauc aaggagagcg guaacacuac cgucacauuc aaccagaaaa      420

uuacaaacaa gaucacaaac cugguuagaa aguagagaau auacaaaaca uuuaauaaga      480

guagaaaacu ggauauuuag aaauccaggu uuugcauugg cugcugcagc uauagcaugg      540

uuacuaggaa gcucaacaag ucagaaagua auauaucuag ucaugauuuu acuaaucgca      600

ccagccuaca guauaaggug uauaggagua ucaaauagag acuuuguaga agguaugagu      660

ggagguacau ggguagacgu aguacuagaa cacggagguu guguaaccgu aauggcccaa      720

gauaaaccga caguagacau agagcuagua acaacaacag uuucaaauau ggcugaaguu      780

agaagcuauu gcuaugaagc cucaauaagc gacauggcua gugauucaag augucccaca      840

cagggagaag cauauuuaga uaaacaguca gacacucaau auguauguaa gagaacauua      900

guagacaggg guuggggaaa uggaugugga uuauucggaa aaggaagucu uguaacaugc      960

gcaaaauuug cauguucgaa gaaaaugaca ggaaaaagua uacaaccaga aaacuuagaa     1020

uauagaauaa ugcuuagugu acauggaagu caacauagug gaaugauagu uaaugauacu     1080

ggucaugaaa cagaugaaaa uagagcaaaa guagaaauaa cuccaaacag cccuagagca     1140

gaagcuacau uaggaggauu ugguaguuua ggacuagauu gcgagccuag aaccgggcug     1200

gacuuuucug auuuauauua cuuaacaaug aacaauaaac auuggcuagu acauaaagaa     1260

ugguuccaug auauaccuuu gccauggcac gcaggggcag auacaggaac accucauugg     1320

aauaacaaag aagcucuagu agaauuuaaa gaugcacaug caaaaagaca aacaguuguu     1380

guauuaggaa gucaagaagg agcaguacac acagcacuag caggagcauu agaagcagaa     1440

auggauggag uaaaaggccg gcuuagcagc ggucaucuaa aauguagauu aaaaauggac     1500

aaacuaaggu uaaaaggugu aaguuauagu cuguguacug cggcauuuac auuuacaaaa     1560

auaccagcag aaacauuaca ugguaccgua acaguagaag uacaauacgc aggaacggau     1620

ggacccugua aaguuccugc acaaauggca guagacaugc aaacacuaac accugugggu     1680

aggcuaauaa cugcaaaucc gguaauaaca gaaucaacug aaaauaguaa aaugaugcuu     1740

gaauuggauc ccccuuuugg agacaguuau auaguaauag ggguaggaga aaagaaaaua     1800

acucaccacu ggcauagaag cggaucaacu auagggaaag cguucgaagc aacaguacga     1860

ggcgcaaaaa gaauggcagu auuaggagac accgcauggg auuuugguag uguaggaggu     1920

gcacuuaaua gccuaggcaa aggcauacac caaauauucg gagcugcauu uaaaucauua     1980

uuuggaggga ugagcugguu cucacaaaua uuaaucggaa cauuauuaau guggcuugga     2040

cuaaauacca aaaauggauc aauaucauuu augugucuag cacuuggcgg aguauuaauu     2100

uuucuaagua cagcaguuuc agcagacgua ggcuguagug uagauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4808
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 1 opt

<400>  4808
gggagaaagc uuaccaugcg uauaauaaau gcacgaaaag aaaaaaaaag aagaggagcu       60

gacacaucag uuggaauugu aggauuauua uuaacuacag caauggcagc agaaguaaca      120

agacgaggau cugcuuauua uauguauuua gauagaaacg augcgggaga agcaauauca      180

uuucccacaa cauugggaau gaauaaaugu uacauacaaa uuauggauuu aggacauaug      240

ugugaugcaa caaugagcua ugaaugucca augcuagacg aaggaguuga accugaugau      300

guagacuguu ggugcaauac uacaucaaca ugggucguau auggaacaug ccaucacaaa      360

aaaggugaag cuagaagaag uagaagagca guaaccuuac caucccacag uacacgaaaa      420

cuacaaacaa ggagucaaac augguuagaa ucaagagaau auaccaaaca ucugauacga      480

guagaaaauu ggauauucag aaacccagga uucgccuugg cagcugccgc gauagcaugg      540

cuacuaggaa guucaacuuc acaaaaagua auauacuuag ucaugauacu acuuauugcg      600

ccagcauaua gcauacgaug uauaggaguc agcaauagag auuuugucga aggaaugucu      660

ggaggaacau ggguagacgu aguauuagaa cauggaggau guguaacagu aauggcacaa      720

gauaaaccaa cuguagauau ugaauuaguu accacaacag uaucaaauau ggcagaagua      780

agaucauacu guuaugaagc aaguauaagc gauauggcaa gugacucaag auguccaacc      840

caaggagaag cauacuuaga uaaacaaagc gauacacaau auguauguaa acgaacguua      900

guagacagag gauggggaaa uggauguggu cuauucggaa aagguaguuu agugacaugu      960

gcaaaauuug cuuguaguaa aaaaaugaca ggaaaaucca uucaaccaga aaaucuagaa     1020

uauagaauaa ugcugagugu acauggauca caacacucug guaugauagu caacgacacu     1080

ggacaugaaa cugaugaaaa uagggcuaaa guagaaauaa caccuaauuc accaagagcc     1140

gaagcuacuu uaggugguuu uggcucauua ggcuuagauu gugaaccacg aacaggauua     1200

gauuuuucag accuauacua ccuaacaaug aacaacaaac auugguuagu ucauaaagaa     1260

ugguuucacg auauaccacu accuuggcau gcaggugcag auacuggaac uccacauugg     1320

aauaacaaag aagcacuagu cgaauuuaaa gacgcacaug caaaacgaca aacaguagua     1380

guuuugggcu cucaagaagg ugcaguucau acagcacuag cuggugcucu agaagcagaa     1440

auggauggag cuaaggguaa acuaaguagc ggacauuuaa aaugcagacu aaaaauggau     1500

aaauuacgac uaaaaggagu aaguuauuca uuaugcacag cggcauucac cuuuacaaaa     1560

auaccagcag aaacauuaca cggaacaguu acaguugaag uccaauaugc aggaacagau     1620

ggaccaugua agguaccugc ccaaauggcu guagauaugc aaacacuuac accuguugga     1680

agacuuauua cagcuaaccc cguaauaacc gagucaacag aaaacaguaa aaugaugcua     1740

gaacuagacc ccccauuugg ugauaguuau auagugauag ggguagguga aaaaaaaaua     1800

acacaucauu ggcauaggag cggaucaaca auagguaaag cauuugaagc cacaguaaga     1860

ggugccaaaa gaauggcagu guuaggagac acagcauggg acuucggauc agugggcggu     1920

gcacuaaaca guuuagguaa gggaauacau cagauauuug gugcugcauu uaaaucuuua     1980

uuuggaggaa ugaguugguu uagucaaauu uuaauuggaa cacuauuaau guggcuaggg     2040

cuaaacacga aaaacggcuc aauaucauua augugcuuag cauuaggugg aguuuuaaua     2100

uuucuaucaa cagcaguuag ugcugacguu ggaugcuccg uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4809
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 1 opt

<400>  4809
gggagaaagc uuaccaugag aauaauaaac gcuagaaaag aaaagaaaag aagaggagcu       60

gauacaagug ucgguaucgu uggacucuua uuaacaacug caauggccgc agaagugaca      120

cgaaggggua gugcauauua cauguauuua gauagaaaug augcaggaga agcgauauca      180

uuccccacaa cacuaggaau gaauaaaugc uauauacaaa uuauggauuu aggucacaug      240

ugugacgcaa caaugucaua ugaaugucca auguuagacg aaggaguaga accagaugau      300

guagauugcu gguguaauac aacaucaaca ugggucguau acggaacuug ucaccacaag      360

aaaggagaag cuaggagaag uagacgagca guaacucuac cuagucauuc uaccagaaaa      420

cuccaaacca gaucacaaac augguuggaa ucaagagaau auacuaaaca ccugauaagg      480

guagaaaauu ggauauuucg uaacccaggu uucgcauuag cagcagcagc aaucgcaugg      540

uuauuaggaa gcagcacauc acaaaaagua auauauuuag ugaugauauu acuaauagcg      600

ccugcauaua guauuagaug uauaggagua ucaaaucgag acuuuguuga aggaauguca      660

ggaggaacau ggguagacgu aguucuugaa cacggaggau guguaacagu aauggcacaa      720

gacaaaccaa cgguagauau agaauuagua acaacgacag uaucaaacau ggcagaagua      780

agaucuuacu guuacgaagc guccauauca gauauggcaa gcgauucaag augcccaacc      840

caaggugaag cauaucuaga uaaacaaagu gauacacaau auguauguaa aaggacucua      900

gucgacagag ggugggguaa uggaugugga cuauuuggaa aaggaucauu aguaacaugu      960

gcaaaauuug cauguucaaa gaaaaugacc ggaaaaagca uccaaccaga aaaucuugaa     1020

uaccgaauua ugcuaucagu acauggauca caacacucag gaaugauagu aaaugacaca     1080

ggucaugaaa cagaugaaaa cagggcaaaa guagaaauaa caccaaauuc accuagagca     1140

gaagcaaccc ucggagguuu uggaucauua ggauuagauu gugaaccuag aacaggacua     1200

gauuuuucag acuuauacua cuuaaccaug aacaauaaac auugguuagu acacaaagaa     1260

ugguuucaug acauaccuuu gccauggcac gcaggagccg auaccggaac accacacugg     1320

aacaauaaag aggcuuuagu agaauuuaaa gaagcacaug caaaacgaca aacaguagua     1380

guauuaggaa gucaagaagg ggccguacac acagcacuag caggagcauu agaagcagaa     1440

auggauggag cuaaaggaag acuaucaagu ggacauuuaa aaugcagacu aaaaauggau     1500

aaacuaagac uaaaaggggu cagcuacagu cuauguacag ccgcauuuac auuuaccaaa     1560

auaccagcag aaacacuaca cggaacagua acaguagaag uacaauacgc aggaacugac     1620

ggaccaugua aaguacccgc acaaauggca guagauaugc agacauuaac uccagucggc     1680

agauuaauaa cugcaaaccc ugucauaaca gaaaguacag aaaauucaaa gaugauguua     1740

gaacuagauc cacccuucgg cgacagcuau aucguuauag gagugggaga aaagaaaaua     1800

acacaucauu ggcaucgauc agguaguaca auaggaaagg cuuuugaagc aacaguaaga     1860

ggugccaaaa gaauggcugu acuuggagau acagcauggg auuuuggauc aguagguggu     1920

gcuuuaaaua guuuaggaaa aggaauacau caaauuuuug gagcagcauu uaaaucauua     1980

uuuggaggaa ugucaugguu cucacaaaua uuaauaggaa cucuauuaau guggcucgga     2040

uuaaauacca aaaauggguc aauaaguuua augugccuag cauuaggggg uguauuaaua     2100

uuuuuaucaa cagcaguaag ugcagauguu ggauguucag ucgauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4810
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 1 opt

<400>  4810
gggagaaagc uuaccaugcg aauaauaaau gcuagaaaag aaaagaaaag aagaggagca       60

gauacuucag uuggaauagu agguuuacuu cucaccacug caauggcagc agaaguaaca      120

cguagaggcu cagcauacua cauguauuua gaucgaaaug augcaggaga agcaauauca      180

uucccaacaa cacucggaau gaauaaaugu uauauacaaa ucauggauuu gggacauaug      240

ugugaugcca cuaugagcua cgaauguccu augcuagacg aaggaguaga accugaugau      300

guagauuguu gguguaacac uacaagcaca uggguaguuu auggcacaug ucaucauaag      360

aaaggugaag caagaagauc aagacgagcg guuacacugc cuucucacuc uacaagaaaa      420

cuacaaacaa gaucacaaac uugguuagaa aguagagaau acacgaaaca uuuaauaaga      480

guagaaaacu ggauuuucag aaaucccgga uuugcuuuag cugcagcagc uauagcuugg      540

uuauuaggaa gcaguacaag ccaaaaagua auauauuuag uaaugauucu acuuauagcu      600

ccagcauauu caauaaggug uauaggaguu ucaaauagag auuuuguaga aggaauguca      660

gguggaacau ggguagaugu cguucuagaa cauggcggau gcguaacagu cauggcacaa      720

gauaaaccaa caguagauau ugaauuagua acaacaacug uaaguaauau ggcagaagua      780

agaucguauu gcuacgaagc aucaauaagu gauauggcau cagacucaag augcccaaca      840

caaggagaag cauaucuaga uaaacaauca gacacgcaau auguuuguaa gcgaaccuua      900

guagauagag guugggguaa cgggugcgga uuauuuggaa aaggcagucu aguaacaugc      960

gcuaaauuug cguguaguaa aaagaugacu ggaaaaucaa uucaaccaga aaaucuagaa     1020

uauagaauaa ugcugucagu acaugguuca caacauagug gaaugauagu aaaugacacg     1080

ggucacgaaa cagaugaaaa uagagcgaaa guagaaauaa caccaaauag cccacgagcu     1140

gaagcuacau ugggagguuu cgguucuuua gguuuagacu gcgaaccgag aacaggauua     1200

gacuucagcg aucuauauua uuuaacaaug aauaauaaac acugguuagu acauaaagaa     1260

ugguuucaug acauuccauu accauggcau gcaggagcag auacagguac accacauugg     1320

aacaauaaag aagcauuagu agaauuuaaa gaugcacaug caaaaagaca aacaguagua     1380

guuuuaggua gucaagaagg agcaguucac acagcacuag cuggugcauu agaagcugaa     1440

auggauggug cgaaaggaag acugaguucc ggacaccuaa aaugcagacu aaaaauggac     1500

aaacuaagac uaaaaggugu aucauacagc uuauguacag cagcauucac auucacaaaa     1560

auucccgcag aaacauuaca uggaacagua acaguugaag uccaguacgc gggugcagau     1620

gguccuugca aaguacccgc ccaaauggca gucgacaugc aaacuuuaac accuguagga     1680

agauugauaa cagcaaaucc aguaauaaca gaaucaacag aaaacaguaa aaugauguug     1740

gaacuugauc caccauucgg ugacaguuac auaguaauag ggguuggaga aaagaaaaua     1800

acacaucauu ggcauagaag uggaagcaca auaggaaaag cauuugaagc aacaguaaga     1860

ggcgcaaaac gcauggcagu acuuggagau acagcuuggg acuuugggag cguuggagga     1920

gcccuaaaca gucuuggaaa aggaauacau cagauauucg gagcugcauu caaaagucua     1980

uuuggaggaa ugucaugguu uagucaaaua uuaaucggua cauuauuaau guggcuaggc     2040

uuaaauacga aaaauggaag uauaagucuu augugucuag cauuaggugg uguacuaaua     2100

uuuuuaagua cugcagugag ugcagacgua gguuguucag uugacuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4811
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 1 opt

<400>  4811
gggagaaagc uuaccaugag aauuauaaau gcaggcaaag aaaaaaaaag acgaggugca       60

gauacuaacg ugggaauagu aggauuauua cuaacaacag cgauggcagc cgaaguaacu      120

agacgaggaa gcgcauauua uauguaucua gaucgaaaug augcgggaga agcaauaagu      180

uucccaacaa cauuaggaau gaacaaaugc uauauacaaa ucauggaucu uggacacaug      240

ugugaugcaa caaugagcua ugaaugucca auguuagaug aaggaguuga accugaugac      300

guggauuguu ggugcaacac gacaucaaca uggguaguau auggaacaug ccaucacaaa      360

aaaggcgaag ccagaagaag cagaagagca guaacguuac cuagucauag cacaaggaaa      420

uuacaaacaa gaagucaaac uuggcuugaa ucaagagaau acacaaagca ucucaucaga      480

guugaaaacu ggaucuuucg aaauccagga uuugcuuuag cagccgcagc cauagcaugg      540

uuacuaggau caucaacaag ucaaaaagua auauacuugg ucaugauacu acuaauugca      600

ccagcuuaua guauacguug cauaggagua aguaacaggg auuuuguaga aggaaugucu      660

ggaggaacau ggguugaugu aguccuagaa cacggaggau guguuacggu aauggcacaa      720

gauaaaccaa caguagauau agaauuagua accaccacag uuucaaauau ggcagaagua      780

cgaucguauu guuacgaagc uaguauaagu gacauggcau cagauucaag augcccaaca      840

caaggagaag ccuauuuaga uaaacaaagu gacacacaau acguauguaa aagaacacua      900

guagauaggg gauggggaaa ugguugcggu uuauuuggga aggguaguuu aguaacgugu      960

gcaaaauuug cauguaguaa aaagaugacu ggcaaaagca uccaaccuga aaauuuagaa     1020

uauagaauaa uguuaucagu acauggaagu caacacucag gaaugaucgu aaacgauacu     1080

ggacacgaaa cugaugaaaa ucgagcaaaa guagaaauua caccaaacuc uccacgagca     1140

gaagcaacau uaggaggauu uggaucauua ggccuagauu gcgaaccccg aacaggacua     1200

gauuucagcg auuuauauua uuuaacaaug aauaauaaac auugguuagu acauaaagag     1260

ugguuucaug acauaccucu accguggcau gcaggagcag auacugguac accacauugg     1320

aauaauaaag aagcacuggu cgaauucaaa gaugcacaug caaaacgaca aacaguagua     1380

guauuaggca gucaagaagg agcgguacau acagcacuag caggagcccu agaagcagaa     1440

auggacggug caaaaggaag auuaaguagu ggacaucuga aauguagauu aaaaauggau     1500

aaacuaagau uaaaaggagu cucauauuca uuauguacag cagcauuuac auuuacaaaa     1560

auacccgccg aaacauuaca uggaacagua acaguggaag uacaauaugc uggaacagau     1620

ggaccuugua aaguuccagc ucaaauggca guagacaugc aaacauuaac acccguaggu     1680

agacuaauua cagcaaaccc uguaauaaca gaaagcacag aaaacucaaa aaugaugcua     1740

gaauuagauc ccccauuugg ugauaguuau auuguuauag gaguuggaga aaaaaaaaua     1800

acacaucauu ggcacagauc cgguucgaca auuggaaaag cauuugaagc uacuguaaga     1860

ggugcaaggc ggauggcggu auugggagac acugcuuggg acuucggauc aguuggagga     1920

gcacuaaacu cacuaggaaa agguauacau caaauuuuug gugcagccuu uaaaucauua     1980

uucggaggca uguccugguu cucacaaaua cuaauaggua cacuucuaau gugguuagga     2040

cuaaauacga aaaauggauc aauaagcuua auguguuuag cccuaggggg aguccuaaua     2100

uucuuaucaa cugcaguauc agcagaugua gguuguagug uagauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4812
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 1 opt

<400>  4812
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaaaaaacg ccgaggagca       60

gaaacuagug uagggauagu agguuuacuu uuaacaacag caauggcagc agaaguuaca      120

agaagaggaa gugcuuauua uauguauuua gaccgcaaag augcaggaga agcaauaagu      180

uuucccacaa cguuggguau gaauaaaugu uacauacaaa uuauggacuu aggacacaug      240

ugcgaugcaa cuaugagcua cgaaugcccu augcuagaug aaggggucga accagaugac      300

guugauuguu gguguaacac aacaucaaca uggguagucu augguacaug ccaucauaaa      360

aaaggugaag cucgacgauc aagaagagca guaacacuac cuagucauag uacuagaaaa      420

cuacaaacaa gaagucaaac auggcuagaa aguagagagu auacaaaaca ccuaauaaga      480

gucgaaaauu ggauuuuuag aaacccagga uuugcauugg cggcagcugc uauugcaugg      540

uuacuaggaa guucaacuuc acaaaaaguu auauaucuag uaaugauacu auuaauagca      600

ccugcguauu caauuagaug uauaggaguu ucgaauagag auuuuguaga agggauguca      660

gguggaacau ggguagacgu aguacuagaa cacggaggau guguaacagu cauggcacaa      720

gauaaaccaa caguugacau agaauuagua acaacaacag uuaguaauau ggcagaaguc      780

agaucauauu gcuaugaagc cagcauaucu gauauggcaa gcgacucaag auguccaaca      840

caaggugaag cguaucuuga uaaacaauca gauacccaau auguauguaa gcgaacauua      900

guagauagag gguggggaaa uggaugcgga cuguuuggaa aaggaagucu agugacaugc      960

gcaaaauucg cauguucaaa aaaaaugaca ggaaaaagca uacaaccgga aaauuuagaa     1020

uauagaauaa uguuaagugu acaugguuca caacauagug gaaugauagu caaugauacu     1080

ggacacgaaa cagaugaaaa ucgagcaaaa guugaaauaa ccccuaauuc accaagagca     1140

gaagcuacau uaggagguuu uggaagucua ggauuagacu gugaaccaag aacagggcua     1200

gacuuuagcg auuuauauua uuuaacaaug aauaauaaac auugguuggu acauaaagaa     1260

ugguuucacg acaucccacu accguggcau gcuggagccg acacugguac accacauugg     1320

aauaauaagg aagcauuagu agaauuuaaa gaugcacacg caaaaaggca aacaguugua     1380

gucuugggca gucaggaggg agcaguucau accgcucuag cuggagcauu agaagcagaa     1440

auggauggag caaaagguag auuaucaucu ggacaccuaa aauguagacu uaaaauggac     1500

aaauugagac uuaaaggugu cucauauucc uuauguacag cugccuuuac auuuacaaaa     1560

aucccagcug aaacauuaca uggaacagug acaguggaag uacaauacgc aggaacagau     1620

ggaccuugua aaguuccagc acaaauggca guugauaugc aaacacuaac accaguuggc     1680

agauuaauua cggccaaccc agugauaaca gaaaguacag aaaauucaaa gaugauguua     1740

gaacuagauc cuccuuuugg agauucauau auugucauag gaguaggaga aaaaaaaaua     1800

acucaucauu ggcauagauc aggaucaaca auaggaaaag cauucgaagc aacaguuaga     1860

ggagcuaaaa ggauggccgu acuaggagac acagcauggg auuuuggauc agugggagga     1920

gcauuaaacu cauugggaaa agggauacac caaauuuuug gcgcagcauu uaaaucuuua     1980

uuugggggca ugucuugguu cagucaaaua uuaauuggaa cacugcucau guggcuugga     2040

cuuaauacaa aaaacggauc aauaagcuua augugucuug cauuaggagg aguucuaaua     2100

uuuuuaagua cugcaguauc ugcagaugua ggauguagug uggauuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4813
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 1 opt

<400>  4813
gggagaaagc uuaccaugag aauaauaaac gcaagaaagg aaaaaaaaag aagaggagca       60

gauacaagug uaggaauagu aggcuuacua cuuaccacag caauggcugc cgaaguaaca      120

agaagaggau cagcauauua cauguauuua gauagaaacg augcaggaga agcaaucucu      180

uuuccaacca cacucgggau gaacaaaugu uacauacaaa uaauggacuu aggacauaug      240

ugugaugcaa cuaugaguua ugaauguccu augcuagaug agggggucga accugaugau      300

guugauuguu gguguaauac cacuagcaca uggguagugu augguacaug ucaucacaaa      360

aaaggugaag caagaagauc aagaagagca guaacauuac caagucauag caccagaaaa      420

uuacaaacaa gaucgcaaac augguuagaa aguagagaau auacuaaaca ucuaauaagg      480

guagaaaacu ggauauuuag aaauccaggc uuugcauuag cagcggcugc aaucgcaugg      540

uuacuaggua guucaacauc acaaaaaguu auauaucuag uuaugauacu auuaauagca      600

ccagcauacu caauaagaug cauaggagua aguaauagag auuucguaga agguaugagu      660

gggggcacau ggguugacgu uguuuuagaa caugguggau guguuacugu aauggcacaa      720

gacaaaccaa cuguagauau agaacuagua acgacaacag uaucaaacau ggcugaagug      780

agaucauauu guuaugaagc caguauaagu gauaugguca gugauucaag auguccaaca      840

caaggugaag cauauuuaga uaaacaauca gacacucaau acguuuguaa aaggacucuc      900

guagacagag gauggggaaa cggaugugga uuauuuggua aagggucuuu aguaacaugc      960

gcaaaauuug cguguagcaa gaaaaugacc ggaaaaagua uucaaccuga aaauuuagag     1020

uauagaauua ugcuaagugu acacggaagu caacauucag guaugauagu uaacgauaca     1080

ggucaugaaa cagaugaaaa uagagcuaaa guagaaauaa caccaaauuc acccagggca     1140

gaagcaacau uagggggcuu cggaucauua ggacuagauu gugaaccucg aacugggcuc     1200

gauuuuagug aucuuuauua uuugacaaug aauaauaaac acuggcuugu gcauaaagaa     1260

ugguuucacg auauaccauu accauggcac gccggagcgg auacaggaac uccacauugg     1320

aacaacaaag aagcauuggu agaauuuaaa gacgcccaug cuaaaagaca aacagucgua     1380

guacuaggau cucaagaagg ugcaguacau acagcgcuag caggagcguu agaagcggaa     1440

auggauggug cuaaaggaag auuaagcagc ggacaucuga aaugcagauu aaaaauggac     1500

aaauuaagau uaaaaggagu cucauauuca uuauguacag cugcauuuac cuucacaaaa     1560

auaccagcag agacuuugca ugguacagua acaguagaag uacaauaugc uggaacagac     1620

ggucccugua aaguaccugc acaaauggcg guagauaugc aaacacuaac ucccguagga     1680

agacuaauaa cagcuaaucc gguuauaacc gaaaguacug aaaauagcaa aaugauguua     1740

gaauuggacc caccauuugg agauaguuau auuguuauag guguaggcga aaaaaaaaua     1800

acucaccauu ggcauagaag ugguaguaca auaggcaaag cauucgaagc aacuguaaga     1860

ggagcaaaaa gaauggcugu acuaggcgau acagcauggg auuucgguag cguaggagga     1920

gcacuaaacu cuuuagguaa agguauucac caaauauuug gugcagcauu uaagucccua     1980

uucggaggaa ugagcugguu uagucaaauc uuaauaggaa cucuacuaau gugguuagga     2040

cuaaauacaa aaaaugguag uauaaguuua augugcuuag cacuuggagg aguauuaauu     2100

uuucuaagca cagcaguauc agcagacguu ggaugcucug uagauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4814
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 1 opt

<400>  4814
gggagaaagc uuaccaugag aauuauaaau gcccgaaaag aaaagaaaag aagaggagca       60

gauacaaaug uagguauagu cggccuacua cuaacaacag caauggcagc agaaguuaca      120

cgccguggau cagcuuauua uauguaccua gauagaaaug augcaggaga agcgauuagu      180

uuuccgacaa cauuagguau gaauaaaugu uacauacaaa uaauggaccu gggacacaug      240

ugcgacgcua caaugagcua ugaaugccca augcuagacg aaggaguaga accagaugac      300

guagacugcu gguguaauau gacaucaaca ugggucguau auggaacaug ccaucauaag      360

aaaggagaag caagaagauc ucggagagcg guaacucucc caagucauag uacuagaaaa      420

cuacaaacaa gaucucaaac cuggcuagaa ucaagagaau auacuaaaca ucuaauaaga      480

guagaaaauu ggauauuuag aaacccuggu uucgcccuag cagcugcugc aauagccugg      540

cuacuaggaa guaguacaag ccaaaaagua auauauuuag uaaugauauu acucauugcu      600

ccggcguaua guauuagaug uauuggagua aguaauagag acuucguaga aggaaugucg      660

ggaggcacau ggguagaugu aguacuagaa cauggaggau guguaacggu uauggcucaa      720

gauaaaccaa caguagauau agaauuaguc acuacaacag uaucaaauau ggcagaagua      780

agaucuuacu guuaugaagc aucaauaagu gauauggcua gcgacucuag augcccaacc      840

caaggagaag cauacuuaga uaaacaauca gauacacaau auguauguaa aagaacauua      900

guagacagag gguggggaaa uggaugugga uuauucggaa aaggaucccu aguaacaugc      960

gcaaaauuug ccuguucaaa gaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa     1020

uauagaauaa uguuaagugu acacgguuca caacauucag gaaugauagu aaaugauacc     1080

ggucaugaaa cugaugaaaa uagagcaaaa guggaaauca ccccaaauuc accaagggca     1140

gaagccacuc uaggaggguu ugguucacua ggacuugauu gugaaccaag aacaggacua     1200

gauuuuucag auuuauauua uuuaaccaug aauaauaaac auugguuagu ucauaaagaa     1260

ugguuucaug auauuccccu uccauggcau gcaggagcag acacaggaac accucauugg     1320

aacaacaaag aagcucuagu agaauuuaaa gaugcacacg caaaaagaca aacuguaguc     1380

guucuaggau cacaagaagg agcaguacac acagcacuag caggagcacu cgaagcagaa     1440

auggauggag caaaaggacg acuaagcagu ggacauuuaa aaugucgacu uaaaauggau     1500

aaauuaagau uaaaaggagu aaguuacuca uuauguacag cagcauuuac auucacaaaa     1560

auacccgcag aaacccuaca uggaacagua acuguggaag uccaauacgc uggcacagau     1620

ggaccaugua aaguaccagc ucaaauggca guagacaugc aaacauuaac uccaguaggu     1680

agauuaauaa cugcaaaucc ugucauaaca gaaucaacgg aaaacuccaa aaugauguua     1740

gaacuagacc caccauuugg agacaguuau auaguuauag guguagguga aaagaaaauu     1800

acacaucauu ggcauagaag uggaucaacc auaggaaaag ccuuugaagc aacaguaaga     1860

ggagccaaaa ggauggcagu acuuggagac acagcauggg acuuuggaag cguaggagga     1920

gcauuaaaca gccugggaaa aggaauacau caaauauucg gagcagcauu uaaaagccuu     1980

uuuggaggua ugucgugguu cucccaaauc cuaauuggaa cucucuuaau guggcuagga     2040

cuuaauacca aaaacggauc aauaucauua augugucuag cauuaggagg uguacuaaua     2100

uuucuaagua cagcaguauc agcagacgua ggauguucag uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4815
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 1 opt

<400>  4815
gggagaaagc uuaccaugag aauaauaaac gcuaggaaag aaaaaaaaag aagaggggca       60

gacacaagug uagggauagu cggacuauua uuaacuacag caauggcagc agaaguuaca      120

aggagagguu cugcauacua uauguauuua gaucgaaaug acgcaggcga ggcuauaagu      180

uuucccacaa cucucggaau gaauaaaugu uauauucaaa uaauggaucu uggacacaug      240

ugugaugcaa ccaugucaua ugaaugucca auguuagaug aaggaguaga gccagacgau      300

guagacuguu gguguaacac uacaucaacu uggguaguuu acggaacaug ucaucauaaa      360

aaaggagaag cgagaaggag uagaagagca guaacauuac cuucacauuc aaccaggaaa      420

uuacaaacaa gaagccaaac cugguuagaa ucaagagaau auacaaagca uuuaauacgc      480

guggaaaacu ggauauuuag gaacccaggc uucgcuuuag cagcggcugc gauagcaugg      540

cuccucggau caucaacguc acaaaaagua auauauuuag uaaugauuuu auugauagca      600

ccagcauauu caauacgaug uauuggcgug ucaaaucgag auuuuguuga aggaaugucc      660

ggaggaacuu ggguagaugu aguauuagaa cauggaggau guguaacagu aauggcacaa      720

gacaaaccua caguugauau agaauuagua acaacaaccg uaucaaauau ggcagaggua      780

agaagcuauu guuaugaagc uucaauauca gauauggcuu cagauagcag auguccaaca      840

cagggagaag cuuaucuaga caaacaauca gacacacaau auguauguaa aaggacacua      900

guagauagag guuggggaaa ugguuguggu cuuuucggua aaggaagcuu aguaaccugc      960

gcaaaauuug cuuguaguaa aaaaaugacu ggaaaaagca uucaaccaga aaauuuagaa     1020

uauagaauaa uguuaagugu acacggcucu cagcauagcg gaaugauagu aaaugauacc     1080

ggccacgaaa cugacgaaaa uagagccaaa gucgaaauua cuccuaauuc uccaagggcu     1140

gaagcugcau uaggugguuu uggaucgcua ggccuugacu gcgaaccaag aacaggauua     1200

gacuucagug aucuauauua uuuaaccaug aauaauaaac acugguuagu ccauaaagaa     1260

ugguuccaug auauaccgcu accauggcac gcaggugcag auacagguac cccacacugg     1320

aauaauaagg aagcauuagu cgaauuuaaa gaugcacacg cgaaaagaca aacaguagua     1380

guauuaggau cacaagaagg ggcuguacac acagcacuag cuggagcacu agaagcagaa     1440

auggauggug ccaaaggcag auuaaguagu ggacaucuua aauguagacu uaaaauggau     1500

aaauuacgac uuaaaggugu aaguuauuca cuauguacag cagcauuuac uuuuacuaaa     1560

auuccagcag aaacacuuca uggcaccgua acuguugaag uacaauacgc aggaacugau     1620

gguccuugua aaguuccagc acaaauggca guggauaugc aaacauuaac cccaguaggc     1680

agauugauaa cugcaaaucc gguaauuaca gaaaguacag aaaacaguaa aaugaugcuu     1740

gagcuugauc caccauuugg ggacucuuac auaguaauag gagucgguga aaaaaaaaua     1800

acacaccacu ggcacagauc agguucaacu auaggaaaag ccuucgaagc uacaguaaga     1860

ggugcaaaaa gaauggcagu auuaggagau acagcauggg acuucggauc uguaggagga     1920

gcauuaaauu cauuaggaaa aggaauacau caaaucuucg gagccgcauu uaaaagucua     1980

uucgguggaa ugucuugguu uagccaaaca uuaauaggca cuuuauuaau guggcuaggu     2040

uuaaauacaa aaaaugguuc aauuaguuua auguguuuag cauuaggagg uguacuaaua     2100

uucuugagua ccgcaguauc agccgauguc gguuguucug uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4816
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 1 opt

<400>  4816
gggagaaagc uuaccaugag gauuauaaac gcacgaaaag aaaaaaaaag aagaggggca       60

gaaacaagcg uaggaauugu aggucuacua uuaacaacag cuauggcugc ugaaguaaca      120

cgaagaggau cagcauauua cauguauuua gauagaaaug augcgggaga agcaaucuca      180

uucccuacaa cguuagggau gaauaaaugu uauauccaaa uaauggaucu aggccacaug      240

ugcgaugcua caauguccua ugagugccca augcuagaug aaggaguaga accagaugau      300

guagauuguu gguguaauac aacaaguaca uggguaguau augguacaug ucaccacaaa      360

aaaggagaag cgaggcgguc aaggagagca guuacauuac caucacacag uacaagaaaa      420

cuacagacac gaucacaaac augguuggaa aguagggaau acacuaaaca ucuaauacgu      480

guugaaaauu ggaucuuuag aaacccaggg uucgcucuag cagcggcagc uauagcaugg      540

uuacuagguu cuucaacauc gcaaaaagua auauaccucg uaaugauucu acuaauagcc      600

uuagcuuacu caauaagaug cauaggcgua uccaauagag auuuuguaga aggaauguca      660

ggaggaacuu ggguagaugu aguuuuagaa cacggaggcu guguaacagu aauggcacag      720

gacaaaccua caguagauau agaauuagua acaacaacag uaucuaauau ggcagaaguu      780

cgaucauauu guuaugaagc aucgauuuca gauauggcaa gcgauaguag auguccuacg      840

caaggugaag cauauuuaga uaaacaaucu gauacucaau auguaugcaa aagaacauua      900

guagauaggg guuggggaaa uggaugugga cuauucggaa aaggaagcuu ggucacuugu      960

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa     1020

uacagaauaa ugcugagcgu acauggaagc caacauagcg gaaugauagu aaacgacaca     1080

ggacaugaaa cagaugaaaa cagagcaaaa guagaaauaa cucccaauuc accaagagca     1140

gaagcaacuc ucggaggauu cggaagcuua ggacuagacu gugaaccgag aacaggucua     1200

gacuucucug aucucuacua ucuaacuaug aauaacaaac auuggcuagu ucauaaagaa     1260

ugguuccaug acauaccauu accauggcau gcuggagccg auacaggaac accacauugg     1320

aacaauaaag aagcucuagu ugaauuuaaa gaugcacaug caaaaagaca aaccgucguu     1380

guauuaggau cacaagaggg agcuguccau acagcauuag caggagcauu agaagccgaa     1440

auggauggag caaaaggaag acuaucauca ggacauuuga aauguagauu aaaaauggau     1500

aaauuaagac uaaaaggggu aucauacuca cuauguaccg cagcauuuac auuuaccaaa     1560

auaccagcag aaacauuaca uggaacaguu acaguggaag uccaauaugc cggaacugau     1620

gguccaugca agguacccgc ccaaauggca guagacaugc aaacacuaac accaguggga     1680

agauuaauaa ccgccaaucc aguaauuaca gaaucaacag aaaauucaaa aaugaugcuc     1740

gaauuagauc cgccauucgg agauagcuac auuguuauug gaguaggaga aaaaaaaauu     1800

acacaccauu ggcaccgaag cggaagcacu auuggaaaag cauuugaagc uacuguaaga     1860

ggagcaaaac gaauggcagu acuuggagau acugcuuggg auuuuggaag uguagguggc     1920

gcacuaaaca gcuuagguaa aggaauacau caaauauucg gcgccgcauu caaaagcuug     1980

uuugggggaa ugucaugguu cucacaaaua uugauaggua cacuuuugau guggcuagga     2040

cuaaauacaa aaaacgguuc aauaagucuu augugucuag cccucggugg aguacuuaua     2100

uuuuuaagua ccgcaguaag cgcagauguu ggcuguagug uagacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4817
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 1 opt

<400>  4817
gggagaaagc uuaccaugag aauaauaaau gcgagaaaag aaaaaaaaag aagaggagcu       60

gauacaagug uuggaauugu gggucucuua uuaacaacag caauggcagc agaaguaaca      120

agaagagggu cagcauacua uauguaucua gauaggaaug augccggcga agcuauauca      180

uuuccuacaa cauuaggaau gaauaaaugc uauauacaaa uaauggaucu uggucauaug      240

ugugaugcaa ccaugaguua ugaauguccg auguuggacg aaggaguuga accagaugau      300

guagacugcu gguguaauac aacaaguaca ugggucguau auggcacaug ucaucauaaa      360

aaaggagaag cgagaagauc gcguagagcu guaacacuac caucacauag uaccagaaaa      420

cuacaaacga gaagucaaac auggcuagaa ucaagagaau auacaaaaca cuuaauacga      480

guagaaaauu ggauauuuag aaacccagga uucgcauuag cagcagcggc aauugcaugg      540

uuauuaggau caaguacgag ucaaaaagua auauauuuag uaaugauacu auuaauagca      600

ccugcauacu caauaagaug uauaggaaua aguaaucgag auuucgugga aggaauguca      660

ggagguacau ggguagaugu aguucuugaa cauggaggau guguaacugu aauggcccaa      720

gacaagccaa caguagauau cgaauuggua acaacgacag uuaguaauau ggcagaagua      780

cgaaguuauu guuacgaagc aucuauuucu gauauggcau cagacaguag auguccaaca      840

caaggagagg cuuaucugga caaacaaagu gacacacaau auguauguaa acguacgcua      900

guugaucgag gauggggaaa cggauguggg uuauucggaa aagggaguuu aguaacaugu      960

gcaaaauuug cauguucgaa aaagaugacg ggaaaaagca uacaaccaga gaauuuggaa     1020

uauagaauaa ugcuaagugu gcacggauca caacacucag guaugauagu caaugauacu     1080

ggacaugaaa ccgaugaaaa ucgugcaaaa guagaaauua caccaaauag uccaagagca     1140

gaagcaacac uaggcggauu cgggauauua ggauuagauu gugaaccaag aacaggacua     1200

gauuuuagcg aucuauacua cuuaaccaug aauaacaaac auugguuagu acauaaagag     1260

ugguuccaug auaucccucu uccauggcac gcuggugcag auacaggaac accacauugg     1320

aacaacaaag aagcacuagu agaauuuaaa gaugcacacg caaaaagaca aacaguagua     1380

guauuaggaa gccaagaggg agcaguacau acagcacuag caggugcauu agaagcagaa     1440

auggacggag cgaaagguag auugaguagu ggacauuuaa aaugcagauu aaaaauggac     1500

aaacuaagac uuaaaggagu aaguuacagu cuuuguacag cagcauuuac uuucacaaaa     1560

auaccagcug aaacacuuca uggaacaguu acuguagaag uucaauaugc agguacagau     1620

ggaccaugua aaguacccgc acaaauggca guagauaugc aaacccugac accaguaggc     1680

agacuaauaa cagcgaaccc aguaauaaca gaaagcaccg aaaacucaaa aaugaugcua     1740

gaauuagacc caccauuugg agacaguuau auaguuauag guguuggaga aaaaaaaaua     1800

acacaucacu ggcacagaag ugguaguaca auaggcaaag cauuugaagc uacugucaga     1860

ggcgcaaaaa gaauggccgu auuaggagau acagcauggg acuuugggag cguuggaggu     1920

gcauugaaua gccucgguaa agggauccau cagauauuug gagcagcauu uaaaagcuua     1980

uucggaggaa ugucaugguu cucacaaaua uuaauuggua cauuauuaau gugguuaggu     2040

uugaacacaa agaaugguuc aauuucauua augugcuuag ccuuaggagg aguauuaaua     2100

uuucuuagua cagcaguauc ggcugauguu ggauguuccg uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4818
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 1 opt

<400>  4818
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaagaaaag aagaggagca       60

gauacaagug uaggaauagu gggucuauua uuaacgacug caauggcagc cgaagugaca      120

agacgaggaa gcgcauauua uauguaucua gauaggaaug augcuggaga agcaauuuca      180

uuuccaacca cuuuaggaau gaauaaaugc uauauucaaa uaauggaucu aggacauaca      240

ugugaugcaa caaugaguua ugaaugucca auguuagaug aaggcguaga accagaugac      300

guagacuguu gguguaauac uacaaguacu uggguagucu auggaacaug ccaucauaag      360

aaaggagaag cucgacgguc acguagagcc guaaccuuac cuucucacuc aauaagaaaa      420

uuacaaacac gaagucaaac auggcuagaa agcagagaau auacaaaaca ucuaauacga      480

gucgaaaauu ggauauuucg aaauccggga uuugccuuag ccgcggcagc aauagcgugg      540

uuacucggua guucaacauc acaaaaagua auuuacuugg uaaugauacu gcuuauagcc      600

ccagccuacu caauaagaug uauaggagua aguaauagag auuuuguuga aggaauguca      660

ggcggaacuu gggucgaugu aguauuagaa cacggcgguu gugugacagu uauggcccaa      720

gacaaaccaa caguagauau agaauuagua acaacuacag uauccaauau ggcagaagua      780

agaaguuauu guuaugaagc aucaauaucg gauauggcau ccgauaguag augucccaca      840

caaggagaag cauaucuaga uaaacaauca gacacacaau auguauguaa acgcaccuua      900

guagacagag guuggggaaa cggaugugga uuauuuggaa aaggaagucu cgugacuugc      960

gcuaaauuug cauguagcaa gaaaaugaca ggcaaaagua uacagccaga aaauuuagaa     1020

uauagaauaa uguuaagugu acauggaagu caacauagug gaaugauugu aaaugauaca     1080

ggacaugaaa cagaugaaaa cagagcaaaa gucgaaauaa caccaaacuc accacgagca     1140

gaagccacac uaggaggauu uggaagccua ggcuuagauu gugaaccaag aacaggacua     1200

gacuuuucag auuuguacua uuuaacuaug aauaauaaac acuggcuagu ucauaaagaa     1260

ugguuccaug acauaccauu accauggcac gcuggugcag auacagggac accacacugg     1320

aauaauaaag aggcuuuggu agaauuuaaa gaugcucacg ccaaaagaca aacaguagua     1380

guauuaggau cacaagaagg agcuguacau acagcauuag cuggugcguu agaagcagaa     1440

auggauggag caaaaggucg auuaagcuca ggacacuuaa aauguagauu aaaaauggac     1500

aaauuaagac uaaaaggugu aucauauagu cuaugcacag cugcauucac auucacaaaa     1560

auacccgccg aaacacuuca ugguacaguc acagucgaag uccaauaugc aggaacugau     1620

ggaccaugca aaguaccugc gcaaauggcu guagacaugc aaacgcuaac uccaguagga     1680

agauuaauaa cagcaaaucc agucauuacu gaaucaacug aaaauaguaa aaugaugcua     1740

gaauuagauc caccauuugg ugauuccuau auaguaauag gcguagguga aaagaaaaua     1800

acucaucauu ggcacagaag ugggucaacu auaggaaagg cuuucgaagc cacaguaaga     1860

ggugcaaaaa gaauggcagu auuaggagac acagcauggg acuuuggaag uguaggagga     1920

gcauuaaaca gucuagguaa aggaauacau caaauauuug gagcagcauu caaaucauua     1980

uucggcggaa ugagcugguu uucacaaaua uuaauaggaa cccucuuaau gugguuagga     2040

cuaaaugcua aaaauggauc aauuucacuu augugucucg cacuaggagg aguccuaaua     2100

uuuuugucga cagcaguauc agcagaugua gguuguucug uggacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4819
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 1 opt

<400>  4819
gggagaaagc uuaccaugag aaucaucaau gcuagaaaag aaaaaaaaag acgcggggca       60

gauacaagug uuggaauugu aggauuguua cuaacaacag caauggcagc agaaguaaca      120

agacgaggga gugcauauua cauguaucua gauaggaaug augcagggga agcuauaagc      180

uuuccaacaa cucuagguau gaauaaaugc uauauacaaa uaauggaccu gggucauaug      240

ugugaugcaa caaugagcua ugaaugucca auguuagaug aaggaguaga accagaugac      300

guagacuguu ggugcaauac aacaaguaca uggguaguau auggaacaug ucaucauaaa      360

aaaggagaag cacaacgaag cagaagagcu guaacacuac caagccacuc uacuagaaaa      420

uuacaaacca gaucacaaac guggcuggaa ucacgugaau auacaaaaca ucuuauuaga      480

guagaaaauu ggauauucag aaauccgggg uuugcauuag cagcagcugc aauagcaugg      540

uuacuaggua guucuacuuc ccaaaaaguc auauacuuag uuaugauauu auuaauagca      600

ccagcuuaca gcauacgaug uauaggcguu aguaauaggg auuuuguaga agguauguca      660

ggaggcacau ggguagaugu aguuuuagaa cacggcggau gugucacugu aauggcgcaa      720

gacaagccaa caguagacau agagcuuguu acaacaacgg uaucaaauau ggcagaagug      780

aggucauauu guuacgaggc aucaauaagu gauauggcau cagauucaag auguccaaca      840

caaggagagg ccuauuuaga uaaacaaagc gauacacaau acguaugcaa aagaacacug      900

guugauagag gauggggaaa uggaugugga cuguuuggaa aaggaaguuu agugacaugc      960

gcgaaauuug ccuguuccaa aaaaaugaca ggaaaaagca uacaaccaga gaauuuagaa     1020

uauagaauaa uguuaagugu gcacggaagu caacacagug gaaugauugu aaaugacaca     1080

ggucacgaaa cagaugaaaa uagagcuaaa guggaaauaa cgccuaauuc accaagggca     1140

gaagcaacau uaggaggauu cggaaguuua gggcuagacu gugaaccaag gacaggguua     1200

gauuuuagcg aucuguauua ucugacgaug aacaacaaac auugguuagu acauaaagaa     1260

ugguuccacg auauaccauu accguggcau gccggagcag acaccggcac accacauugg     1320

aauaauaaag aagcauuagu agaauuuaaa gacgcacaug caaaacguca aacaguagua     1380

guucuaggaa gucaagaagg ugcaguacac acugcauuag ccggagcacu agaggcggaa     1440

auggacggag caaaaggucg acuuucaagu ggucaucuaa aauguagauu aaaaauggac     1500

aaacuucgac uaaaaggagu aucuuauagu uuaugcacag cagcauucac auuuacgaaa     1560

auuccagcag aaacauuaca uggaacagug acuguagagg uacaguacgc gggaacagau     1620

ggaccaugua aagugccagc acaaauggca guugacaugc aaacauugac accaguagga     1680

cgacucauca cagcaaaucc cguaauaacc gaaucaacgg aaaauucaaa aaugauguug     1740

gaauuagacc caccauuugg ggacaguuau auuguaauag gaguuggaga aaaaaaaauu     1800

acucaucacu ggcacaggag cggaucaaca auagguaaag cguucgaagc aacuguucga     1860

ggagccaaac gaauggcagu auuaggagau acagcauggg auuuugguuc aguaggagga     1920

gcauuaaaca guuuaggaaa aggaauacac caaauauuug gagcggcauu uaagucauug     1980

uuuggaggga ugucaugguu cucacaaaua cuaauaggaa cacuguuaau gugguuagga     2040

uuaaauacca aaaacggcag uauuaguuua augugcuuag cacuaggagg aguguuaaua     2100

uucuuaagua cugcuguaag ugccgaugua ggcuguagug ucgacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4820
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 1 opt

<400>  4820
gggagaaagc uuaccaugag aauaauaaac gcaagaaaag aaaaaaagag aagaggagca       60

gauaccagug uagguauugu aggucuguua uugacaacag cuauggcagc agaaguaaca      120

agaagaggaa gugcuuauua cauguauuua gauagaaaug augcaggaga agcaauauca      180

uucccaacaa cauuaggaau gaauaaaugc uauauacaga uaauggacuu aggacacaug      240

ugugaugcaa cuaugucgua ugaaugucca augcuagaug aaggaguaga accggaugau      300

guugauugcu gguguaauac aacgucaaca uggguaguuu auggaaccug ucaucauaag      360

aaaggggaag cuagacguuc aagaagagca guaacccuac caucacacag cacgcgaaaa      420

uuacaagcaa gaucacagac uugguuagaa ucacgagaau auacgaaaca cuuaauacga      480

guagagaauu ggauauuuag aaauccagga uuugcauuag cagcagccgc uauagcaugg      540

uuacuuggau caucaacauc ccaaaaagua aucuaccuag uaaugauacu auuaauagca      600

ccagcuuaua guauuagaug uauaggagua aguaauagag auuuuguuga aggaaugagu      660

gguggaacgu ggguagaugu aguauuagaa cauggcggau guguaaccgu uauggcacaa      720

gacaaaccaa caguggacau agaacuaguu acaacaacug uaagcaacau ggcggaagua      780

aggucauauu guuacgaagc uagcauaagu gacauggcau cagauaguag auguccaaca      840

caaggcgaag ccuaucuaga uaaacaauca gacacacaau auguauguaa gagaacacuu      900

guagaucgug gauggggaaa cgguugugga uuguuuggua aaggaucacu aguuacuugu      960

gcaaaauucg cauguaguaa aaaaaugacg ggaaaaagca uacaaccaga aaauuuagag     1020

uacagaauaa ugcugagugu ucaugguuca caacauucag gaaugauagu aaacgauaca     1080

ggucacgaaa cugaugaaaa cagagcgaaa guagaaauaa cuccaaauag uccuagagcc     1140

gaagcaacuu uaggcgguuu ugguucauua ggauuagacu gugaaccaag aacuggauua     1200

gauuucucug auuuauacua cuuaacuaug aauaauaaac auuggcuagu ccauaaagaa     1260

ugguuucaug acauaccauu acccuggcac gcaggugccg acacagguac accacauugg     1320

aauaauaaag aagcauuagu ugaauuuaaa gaugcacaug caaaaagaca aacaguagua     1380

guauuaggga gucaagaagg agcaguacau acagcuuuag caggggcauu ggaagcagaa     1440

auggauggcg cuaaagguag acuaucaucu ggacacuuaa aaugucgacu aaaaauggau     1500

aaauuaagac uuaaaggagu aaguuauagc cuaugcacgg cagcguuuac auuuacaaaa     1560

auaccugcug aaacuuuaca uggcacaguu acaguagaag uacaguaugc gggaacagau     1620

ggucccugua aaguaccagc ucaaauggcu guagauaugc aaacguuaac accaguaggc     1680

agauuaauaa cagcaaaccc aguuauaaca gaaaguaccg aaaauagcaa aaugauguug     1740

gaauuagauc caccuuuugg agauucauau auaguaauag gaguuggaga aaaaaaaaua     1800

acacaucauu ggcauagaag uggaucaacc auaggcaaag cuuuugaggc aacaguaagg     1860

ggagcaaaac gaauggcagu auuaggagau acagcauggg auuuugguuc aguaggagga     1920

gcacucaaua gucuuggcaa aggcauacau cagauauuug gagccgcuuu caaaucauua     1980

uuugguggga ugucaugguu cucacaaauu cugauaggaa cacuguuaau gugguuaggc     2040

cuuaauacga aaaauggauc aauuagcuua augugcuuag cauuaggagg aguacuaauu     2100

uuuuuaucaa ccgcaguaag cgcugaugua gggugcagug uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4821
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 1 opt

<400>  4821
gggagaaagc uuaccaugag aauaauaaau gcacgaaaag agaagaaaag acggggcgca       60

gacacaagug ucggaauugu aggauuacuu cuaacaacag caauggcagc agaaguaaca      120

agaagaggaa gugcauauua uauguaucug gauagaagcg augcaggaga agcaauuagc      180

uucccaacaa cguuagguau gaauaaaugc uacauacaaa uaauggauuu aggacauaug      240

ugcgacgcaa caaugaguua cgaaugucca augcuggaug aaggaguaga accagacgau      300

guggauuguu ggugcaacac uacaaguacu ugggugguau auggaacuug ccaucacaag      360

aaaggugaag caagaagauc aaggagagcu guuacguuac caucacacag uacaagaaaa      420

uuacaaacaa gaagucaaac auggcuggaa aguagagaau acacaaaaca cuuaauaaga      480

guugaaaauu ggaucuuuag gaacccagga uuugcacuag cugcagcagc aauagccugg      540

cuacuaggua guaguacaag ccaaaaagua auauauuuag ucaugauccu auuaauagca      600

ccagcauacu cuauaagaug uauaggagua agcaauagag auuucguaga aggaaugucu      660

ggaggaaccu ggguagaugu aguuuuagaa cacggaggcu gcguaacagu uauggcccaa      720

gauaaaccga caguugauau agaacuugua acaacaacgg uaaguaauau ggcagaagua      780

agauccuacu guuacgaagc aaguauaagu gacauggcuu cagauucaag auguccaaca      840

caaggggaag cguaccuuga uaaacaaucu gauacacaau auguuuguaa aagaacccua      900

guagauagag guuggggaaa uggaugugga cuuuuuggaa aagggaguuu aguuacaugu      960

gcaaaauucg cauguaguaa gaaaaugaca gguaaaagua uacaaccaga aaauuuggaa     1020

uacaggauaa uguuaagugu ccauggaagu caacauagcg gaaugauagu caaugauacg     1080

gggcaugaaa cagacgaaaa cagagcaaaa guagaaauaa caccuaauuc cccgcgagca     1140

gaagcaacau uagguggauu uggcucauua ggauuagauu gcgaaccaag gacuggauua     1200

gauuuuagug auuuguauua uuuaacaaug aacaauaaac auuggcuugu ucauaaagaa     1260

ugguuucaug auauuccacu accauggcac gcaggagcug auacaggcac cccacauugg     1320

aauaauaaag aagcauuggu cgaauuuaaa gaugcucaug caaaaagaca aacgguagua     1380

guauuaggau cacaagaagg agcaguacau acagcacuug cuggugcuuu agaagcagaa     1440

auggauggag caaaaggucg auuaaguagu ggacauuuaa aauguagauu aaaaauggac     1500

aaauugagac uaaaaggugu cuccuauagc uuaugcacag cagcauuuac auuuacaaaa     1560

aucccagcag aaacauuaca uggaacugua acuguugaag uacaauaugc agguacagau     1620

ggcccgugua aaguuccagc ucagauggcu guugauaugc aaacauuaac accugucgga     1680

agauuaauaa cggcaaaucc cguaaucacg gaaaguacug aaaauaguaa aaugaugcua     1740

gaauuagauc caccauuugg ggacagcuau auaguaauag gaguagguga aaagaaaauc     1800

acacaucauu ggcauaggag uggaagcaca auaggaaaag ccuuugaagc aacaguaaga     1860

ggagcaaaac gaauggccgu gcuaggugau acagcauggg auuucggauc agugggugga     1920

gcucucaauu ccuuaggaaa agggauacau caaauauuug gagcugcauu uaaaagucua     1980

uuugggggua ugucuugguu uagucaaaua uuaauuggaa cauuacuuau guggcuggga     2040

uuaaacacua aaaaugguuc gauaaguuua auguguuuag cauuaggugg gguuuuaauu     2100

uuucuaagua cagcaguauc cgccgaugug gguuguagug uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4822
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 1 opt

<400>  4822
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaagaaaag aagagguaca       60

gacaccagug uaggaauagu aggauuacua uugacaacag caauggcugc agaaguuaca      120

cgcagaggau cugcuuacua uauguaccua gauagaagug augcaggaga agccauuuca      180

uucccaacaa cuuuagguau gaauaaaugu uauauacaaa uaauggaucu aggacacaug      240

ugcgacgcaa cuaugaguua ugaaugcccu augcuagaug aaggaguaga acccgaugau      300

guugauuguu gguguaacac aacaaguaca uggguaguau acggaacaug ucaucauaag      360

aaaggcgaag caagaagaag cagacgagca guuacauuac cgagccauuc aacaagaaaa      420

cuacaaacaa gaucucaaac cugguuagaa agucgagaau auacaaaaca uuuaauuaga      480

guagaaaacu ggauauucag aaauccagga uuugcuuuag cagcugcagc uauagcaugg      540

cuacuaggau cauccacauc acaaaaaguu auauacuuag uaaugauucu auuaauagcc      600

ccagcauauu caauaagaug cauaggugua aguaauagag auuucgucga aggaaugagu      660

ggaggaacau ggguugacgu aguacuagaa cauggaggcu guguaacagu aauggcccaa      720

gauaaaccga cuguagauau agaauuagua acaacaacag uaaguaauau ggccgaaguc      780

agaucauacu guuaugaagc cagcauaagu gauauggcaa gcgauuccag auguccaacu      840

caaggugagg cauauuuaga uaaacaaucg gauacacaau augugugcaa aagaacucua      900

guagacagag gauggggaaa cggcugugga uuauuuggaa aaggaagucu aguaacaugu      960

gccaaauuug caugcucaaa gaaaaugacu ggaaaaucaa uacaaccaga aaaucuagaa     1020

uaucgaauaa uguuaagugu gcauggaagu caacacagcg gaaugauagu aaacgauacu     1080

ggucaugaga cagacgaaaa cagagcaaaa guagaaauaa caccuaauuc accacgagca     1140

gaagcaaccu uaggaggcuu cggaucccua ggauuagauu gugaaccacg aacaggcuua     1200

gacuuuagug auuuauauua uuuaacaaug aauaacaaac auuggcuggu acauaaagaa     1260

ugguuucacg auaucccauu accauggcac acaggugcag auacaggaac accacauugg     1320

aacaacaaag aggcgcuugu agaauuuaaa gaugcucacg caaaaagaca aacaguugua     1380

guauugggau cucaagaagg cgcaguacau acagcauuag cuggagcucu agaagcagaa     1440

auggacggug caaaaggccg acugucuucu ggucacuuaa aaugcagacu caaaauggau     1500

aaauuaagac uuaaaggcgu aucauacagu uuauguaccg cugcuuucac auuuacaaaa     1560

auaccagcug aaacuuuaca uggaaccgua acaguagaag ugcaauacgc ugguacugau     1620

ggaccaugua aagugcccgc ucaaauggca guagauaugc agacacuaac accaguggga     1680

cgauuaauua cugcaaaucc aguuauuaca gaaggaacag aaaacucaaa aaugauguua     1740

gaauuagauc caccauuugg ugauucauau auaguaauag gaguagguga gaaaaagauu     1800

acacaucauu ggcauagauc aggaucaaca auagggaaag cguucgaagc uacaguaaga     1860

ggagcuaaaa gaauggcagu auuaggagac acggcauggg auuuuggauc agugggugga     1920

guacucaauu cauugggaaa aggaauacac caaauauucg gagcagccuu caaaagccua     1980

uuuggaggaa ugaguugguu uagucaaaua cucauaggaa cauuguuaau guggcuaggc     2040

uuaaauacua aaaauggcag uauaucacua augugucuug cacugggagg aguacuuaua     2100

uuucuaucaa cagcuguauc cgcagaugua ggauguagcg uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4823
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 1 opt

<400>  4823
gggagaaagc uuaccaugag aauuauaaau gcaaggaaag aaaaaaaaag aaggggagcu       60

gauacaucag uaggaauagu uggucuucua cuaacuacag caauggcagc ggaaguaacg      120

cgaagaggau cagcauauua uauguaucua gauagaaaug augcaggaga ggcaaucucu      180

uuuccuacaa cauuaggaau gaacaaaugu uacauacaaa uaauggacuu gggacauaug      240

ugcgaugcaa ccaugucaua ugaauguccu auguuggaug aagguguaga accugaugac      300

guugauuguu ggugcaauac aacaucaaca uggguaguau acgggacaug ucaucacaaa      360

aaaggugaag cuagaagauc aagaagagcu guuacauuac cuucacacuc aacacgaaaa      420

uuacaaacaa gaucacaaac augguuagaa ucaagagaau auacaaaaca ccuaauaaga      480

guagaaaauu ggauauuuag aaauccaggu uuugcauuag cagcagcagc aauagcaugg      540

uuauuaggau ccucaacgag ucaaaaaguu auuuauuuag uaaugauuuu guuaauugcu      600

ccagcuuauu caauaagaug cauaggugua aguaaucgag acuuuguaga agggaugagu      660

ggugggacau ggguagaugu aguauuagaa cacggcggau gcguaacggc aauggcgcaa      720

gacaaaccaa caguagauau agaacuaguu acaacuacgg uuucuaacau ggcagaagua      780

agaucuuauu gcuacgaagc aucaauauca gacauggcau cugauaguag auguccaaca      840

caaggggaag cauaucuaga uaaacaauca gauacacaau auguauguaa acgaacucua      900

guagaccgug gcugggguaa cggaugcggg cucuuuggaa aaggaucauu aguaacaugu      960

gcaaaauuug cuuguuccaa gaaaaugaca ggaaaaagca uacaaccaga aaaccuugaa     1020

uauaggauaa uguuaucagu acauggcuca caacauagug guaugcuugu aaaugauaca     1080

ggacaugaaa cggacgaaaa ccgagcaaaa guugaaauaa cuccaaauag uccgcgagcu     1140

gaagcaacgu uaggcggauu uggaagcuua gggcuagauu gugaaccaag aacaggauug     1200

gauuucucag aucuuuauua uuuaacaaug aauaauaaac auugguuagc acacaaggaa     1260

ugguuccaug auauaccccu accauggcau gcaggugcag ccacgggaac uccucauugg     1320

aacaauaaag aagcauuagu agaauuuaaa gacgcacacg ccaaaagaca aaccgucguu     1380

guacuuggau cacaagaggg agcaguacau acagcauuag caggagcauu ggaagcagaa     1440

auggauggug cuaaaggaag auuaucuagc ggacaucuaa aaugcaggcu aaaaauggac     1500

aaacuacgau uaaaaggugu aagcuauagu cuaugcacag cagcuuuuac auuuacaaaa     1560

auacccgcag agacuguuga cgguacagua acaguagaag gucaauaugg agggacugau     1620

gguccuugca aaguuccagc acaaauggca gucgacaugc aaacacuaac gccaguuggu     1680

agacuaauaa cagcgaaucc cgucauuaca gaaucaacag aaaauucuaa aaugauguua     1740

gaauuagauc caccauuugg cgauagcuau auaguuaucg gaguaggcga aaaaaaaaua     1800

acucaucauu ggcaccggag uggaagcacu auaggaaaag cauucgaagc cacaguaagg     1860

ggugcaaaaa gaauggcagu gcuaggagau acugcauggg auuuuggaag uguagggggc     1920

gcauuaaaua guuuaggaaa aggaauacau caaauaauag gugcagccuu uaaaagucua     1980

uucggaggaa ugaguugguu uagccaaaua uuaauaggaa ccuuacugau gugguuagga     2040

cuuaacacga aaaauggaag cauaucacuu augugucucg ccuugggagg aguauuaaua     2100

uuucuaucga cggcaguaag cggcggggua gguuguucag uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4824
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 1 opt

<400>  4824
gggagaaagc uuaccaugag aauaauaaac gcaagaaaag aaaaaaaaag gagagguaca       60

gauacauccg ucggaauagu ggguuuauua cuuacaacug caauggcggc agaaguaaca      120

cgaagaggaa gcgcauauua uauguauuua gaucgaagug augcgggaga agcaauaagc      180

uucccaacaa cauuaggaau gaacaaaugu uauauacaaa uaauggauuu aggacacaug      240

ugugaugcaa cgaugucaua ugaaugucca auguuagaug aaggaguaga accggaugau      300

gucgauuguu gguguaauac aacaucaaca uggguaguau auggaaccug ccaucauaaa      360

aaaggugaag caagaagauc uaggagagca guaacgcuuc caagucauag uacaaggaaa      420

uuacaaacua gaagucaaac augguuagaa aguagagaau acacuaaaca uuuaauacga      480

guagaaaacu ggauauuuag aaauccaggc uucgcauuag ccgcagcugc cauagcaugg      540

uuauuagguu cgaguacguc acaaaaaguu auauauuuag uuaugauucu auuaauugcc      600

cccgcauaua guauaagaug cauaggagug aguaaccggg auuuuguuga aggaauguca      660

ggaggaacau gggucgaugu uguauuagaa caugguggau gcguaacagu uauggcacaa      720

gacaaaccaa caguggacau agaauuagua acaacaacgg uaaguaauau ggcagaagua      780

cgaaguuacu guuaugaagc aaguauauca gauauggcaa gcgauucuag auguccaacu      840

caaggcgaag cauaucuaga uaaacaaucc gauacacaau acgugugcaa gaggacguug      900

guagauagag gaugggguaa ugggugcggg uuauucggaa aagguucauu aguaaccugc      960

gcuaaauucg cguguucuaa aaaaaugaca ggaaaaucaa uacaaccgga aaauuuagag     1020

uauagaauaa uguugucagu ucauggaagu caacauucag gaaugauagu aaaugauacu     1080

ggacaugaaa cagacgagaa ccgagcaaaa guugaaauaa caccaaauag uccaagggca     1140

gaagcuacau uaggaggauu uggcucucua ggacuggauu gugaaccaag aacuggcuua     1200

gauuuuagcg accuauauua ccuaacgaug aauaauaagc acuggcuagu acacaaagaa     1260

ugguuccacg auauaccauu accauggcau gcaggugcag auacaggaac uccacauugg     1320

aauaauaaag aagcacuagu ggaguuuaaa gacgcacaug caaaaaggca aacaguugua     1380

guuuuaggca gucaagaagg cgcuguacau acagcauuag caggagcacu agaagcggaa     1440

auggauggag caaaaggaag auuaucaagc ggacaucuaa aauguagguu aaaaauggau     1500

aagcuuagau uaaaaggagu caguuacuca uuauguacgg cagcuuuuac cuuuagcaaa     1560

auaccagcag aaacacuaca cggcacaguu acugaagaag ugcaauaugc uggaacugau     1620

ggcccaugca aaguaccggc acaaauggca guagauaugc aaacacuaac accaguagga     1680

agauuaauaa cagcaaaccc aguaauaaca gaaucaacag aaaacaguaa aaugauguua     1740

gaauuagacc caccauuugg agauucauau auagucauag gaguaggaga aaaaaaaauu     1800

acccaucauu ggcauagaag ugguaguaca auaggcaaag cauucgaagc uacaguaaga     1860

ggagcaaaaa ggauggcagu cuuaggagac acagcauggg auuuuggauc aguaggagga     1920

gcuuuaaaua guuuaggaaa aggaauucau cagauauucg gagcagcauu uaaaagccua     1980

uucgguggaa ugagcugguu uucgcaaaua uugauaggaa cauuacuaau gugguuagga     2040

uuaaacacaa aaaacggauc uauaagucug augugccuag cguuaggggg cguucuaaua     2100

uuccucucaa cagcaguauc agcggacgua ggauguucag uagauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4825
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 1 opt

<400>  4825
gggagaaagc uuaccaugag aauaauaaau gcacgaaaag aaaaaaaaag aagaggaaca       60

gacacuucag uaggcauugu aggacuacua cuuacaacag caauggcagc agagguaaca      120

cgaaggggaa gugcauauua uauguauuua gacagaucag augcaggaga agcgaucuca      180

uuuccuacaa cauugggaau gaauaaaugu uacauccaaa uaauggaucu gggccauaug      240

ugugaugcaa caaugaguua cgaaugucca auguuagaug aaggggucga gccagaugau      300

guagauuguu gguguaauac cacuucaaca uggguuguuu auggaacaug ccaucauaaa      360

aaaggagaag caagaagauc cagaagggca guaacauuac caucacauuc uacacgaaaa      420

uuacaaaccc gaucgcaaac auggcuagaa aguagagaau acaccaaaca uuuaauaaga      480

gucgaaaauu ggauauucag aaacccaggc uuugcuuuag cagcagcugc gauagccugg      540

cuacuaggaa guagcacuuc acaaaaaguc aucuauuuag uaaugauuuu acuaaucgcu      600

ccagcauaca gcauuagaug uauuggaguu aguaauagag acuuuguaga aggaauguca      660

ggcggaacau ggguugaugu gguccuagaa cauggcggau guguaacagu aauggcccaa      720

gauaaaccca caguagauau agaauuagua acgacaacag ugagcaauau ggcagaagua      780

agaucauauu gcuacgaagc uucaauaucu gauauggcaa gcgauucgag augcccaaca      840

caaggagaag cauaucucga uaaacaaagc gacacucaau auguaugcaa aagaacacua      900

guagacagag gcugggguaa ugguuguggu uuguuuggaa aagggaguuu aguaacaugu      960

gcuaaauucg caugcucgaa gaaaaugaca ggcaaaucaa uacaaccuga aaauuuagaa     1020

uacagaauaa ugcuaagugu acaugguuca caacauucag guaugauugu aaaugauaca     1080

ggucacgaaa cagaugaaaa uagagcuaaa guagaaauaa cccccaauuc accaagagcu     1140

gaagccacac uaggaggcuu cgguaguuua ggguuagauu gugaaccuag aacagggcuu     1200

gauuuuucag accuuuauua ucucacaaug aauaauaaac acuggcucgu acauaaagaa     1260

ugguuccaug acauaccucu acccuggcac gcuggagcag auacaggaac gccacacugg     1320

aauaauaaag aagcuuuagu agaauucaaa gaugcccacg ccaaaagaca aacuguagua     1380

guacuaggaa gucaagaagg agcaguacac acagcuuuag caggagcauu agaagcggaa     1440

auggacggag cgaaaggaag auuaucaucu ggucauuuaa aauguagauu gaaaauggau     1500

aaacuaagau uaaaaggugu uucauauagc uuaugcacag cugcauuuac auuuacaaaa     1560

auaccagcag aaacuuuaca uggcacagua acaguagaag uccaguaugc agguacugau     1620

ggaccuugua agguacccgc acaaauggca guagauaugc aaacacucac accaguagga     1680

agacuaauua cagcuaaucc aguuauuaca gaaagcacag aaaauuccaa aaugaugcua     1740

gaguuagauc caccuuuugg ugacaguuau auagugauag gggucggaga aaaaaaaaua     1800

acacaucacu ggcauagaag cggaucaaca auaggaaaag cauuugaagc cacaguaaga     1860

ggagcuaaaa gaauggcugu acuaggcgau acagcauggg auuucggaag uguuggcgga     1920

gcucugaaca gccucgggaa agguauacau caaauauuug gugcggcauu uaaaaguuua     1980

uuuggaggaa ugucaugguu uucacaaaua uuaauaggaa cacuacuaau gugguuaggu     2040

cucaauacua aaaauggaag uauaagcuua auguguuuag cauugggagg agucuuaaua     2100

uuccuaucaa cagcaguaag ugcagaugua ggauguagug uugauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4826
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 1 opt

<400>  4826
gggagaaagc uuaccaugag aaucauuaau gcacguaaag aaaagaaaag aaggggaaca       60

gauacgucug uaggaauagu uggacuacua uuaacaacug caauggcugu agaaguaaca      120

agaagaggaa augccuauua uauguauuua gauaggucgg acgcaggaga agcaauuuca      180

uucccaacaa caaugggaau gaacaaaugu uauauccaaa ucauggaccu uggacauaug      240

ugugacgcaa caaugucaua ugaaugucca auguuagaug aagggguuga accagaugau      300

guagauuguu ggugcaacac aacaagcaca uggguagucu acggaacaug ucaucauaag      360

aaaggagaag cacgaagauc aagacgagca guuacauuac caagucacuc aacaagaaaa      420

uuacaaacaa gaucacaaac auggcucgaa aguagagaau acaccaaaca ucugauccga      480

guagaaaacu ggauauuuag aaaucccggu uuugcucuug cugccgccgc aaucgcaugg      540

uuacuaggau cgucuacuuc acaaaaaguu auauauuuag uaaugauauu auugauagcu      600

ccagcauauu ccauuagaug cauaggaguu uccaauagag auuuuguaga agggaugagc      660

ggagguaccu ggguagaugu aguauuagaa cacggaggau gcguuacagu aauggcacaa      720

gacaaaccaa caguagacau agaauuaguu acaacaacag uaucaaacau ggcagaaguu      780

cguuccuauu gcuacgaagc aaguauuuca gauauggcaa gugauaguag auguccgaca      840

caaggagaag cauauuuaga caaacaauca gacacucaau auguauguaa aagaacucua      900

guagauagag guuggggaaa uggcugugga uuauucggca aaggaucucu aguaacaugu      960

gcaaaauuug ccuguaguaa gaaaaugaca ggaaaaucca uccaaccaga aaauuuagaa     1020

uaucgaauaa uguuaucagu acauggauca caacauuccg gaaugaucgu caacgauacu     1080

ggucaugaga cugaugaaaa uagagcaaaa guagaaauaa caccaaauuc accucgagcu     1140

gaagcaacau ugggaggauu uggaucacua ggacuagacu gugaaccaag aacaggacuu     1200

gauuuuagcg acuuauauua ccuuacgaug aacaauaaac auugguuagu acauaaagaa     1260

ugguuucacg acauaccacu accuuggcau gcaggagcag auacgggaac uccccauugg     1320

aacaauaaag aggcuuuagu agaguucaaa gacgcacacg caaaaagaca aacaguagua     1380

guucuuggaa gucaagaagg agcuguacac acagccuuag cuggugcacu agaagcagaa     1440

auggauggag caaaaggaag auuaaguagu ggacacuuaa aauguagauu aaaaauggau     1500

aaauuacgau uaaaaggagu uucauauuca uuaugcacag cagcuuuuac guucacgaag     1560

auaccagcag aaacacugca cggaacagua acaguagaag uacaauaugc cgguacagau     1620

ggaccuugua agguaccagc acaaauggcu guugacaugc aaacauuaac ucccguuggu     1680

agacuaauaa cagcaaaucc aguaauaacu gaaagcacag aaaauucuaa aaugaugcua     1740

gaauuagauc caccuuuugg agauagcuac aucguaauag gaguuggcga aaagaaaaua     1800

acucaucacu ggcaccguuc agggucaacc auugguaaag cauuugaagc aacaguaaga     1860

ggagccaaaa gaauggcggu acuaggagau acggcauggg auuuuggaag uguaggaggu     1920

gcccuaaauu ccuuggguaa aggaauacac caaaucuuug gagccgcauu uaaaucauua     1980

uucgggggaa ugaguugguu cagccaaaua uuaauuggaa cacuauuagu augguuagga     2040

uugaauacua aaaauggauc uauaucacua augugccuag cauuaggagg agugcucaua     2100

uuccuaagua cugcuguuuc agcagaugua ggauguagug uagauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4827
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 1 opt

<400>  4827
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaaaaaacg aagaggaaca       60

gauacaagcg uggguauagu uggcuugcua uugacuacug caauggcagc ugaaguaacc      120

agaagaggaa gugcauacua cauguaucua gaucguucug augccgguga agcaaucuca      180

uuuccuacaa cauuagguau gaacaaaugu uauauacaaa uaauggaucu aggucauaug      240

ugugaugcaa caaugucuua ugaauguccu augcuggacg agggaguuga accagaugac      300

gucgauugcu ggugcaauac cacguccaca uggguaguuu auggaacuug ucaccacaaa      360

aaaggagaag caagaagaag uagaagagca guaacgcuac caagucauag uacaagaaaa      420

cuccaaacaa ggucacagac uuggcucgaa aguagggaau auacuaaaca ucuaauacga      480

guagaaaauu ggauauuuag aaauccaggg uuugcguuag cagcagcagc aauagcuugg      540

uuauuaggcu ccaguacuuc acaaaaagua aucuacuuag uaaugauauu auuaauagcc      600

ccagcauauu caauaagaug uauagguguu aguaauagag auuuuguaga aggaauguca      660

ggagguacuu ggguagaugu cguucuagaa cacggaggau gcguaacagu aauggcacaa      720

gauaaaccca cuguagauau agaacuaguu acaacaacug uauccaauau ggcagaagua      780

agaaguuauu guuacgaagc uagcauaagc gauauggcaa gugacucaag augucccaca      840

caaggugaag cauaccuuga uaaacaauca gauacucaau auguaugcaa acguacacug      900

guagaccgug gaugggggaa uggaugcgga cuauucggua aaggauccuu aguuacaugu      960

gcaaaauuug cuuguucaaa aaaaaugacu gggaaaucaa uacaaccaga aaacuuagaa     1020

uauagaauua uguuaucggu acacggauca cagcauucug gaaugauagu aaaugauaca     1080

ggacaugaaa cagaugaaaa ucgagcuaaa guagaaauaa cuccuaauag uccaagagcg     1140

gaagcaacuc uaggagguuu uggaagucua ggauuagauu gcgaaccgag aacuggguua     1200

gacuuuucag auuuauauua ucuaacuaug aacaacaagc acugguuagu acauaaagag     1260

ugguuucaug auauaccgcu accauggcau gcgggagcug auacaggaac uccacauugg     1320

aauaauaaag aagcacuagu ggaauuuaaa gaugcacaug caaaacgaca aaccguggua     1380

guacuaggau cacaagaagg agcaguacau acagccuuag cuggugcauu agaagcagaa     1440

auggauggag caaaaggaag auuaaguagu ggucaucuaa aauguagacu aaagauggac     1500

aaauuaagau uaaagggagu gucuuacagu cuauguacag cagccuuuac auuuacaaaa     1560

auaccugcug aaacauuaca cggaacagua acuguggaag uacaauaugc agguaccgau     1620

ggaccaugua aaguuccugc acaaauggcu guagauaugc aaacucucac accgguagga     1680

cgacuaauaa cagcaaaucc aguaaucaca gaaaguacug aaaauaguaa aaugaugcuc     1740

gaguuagauc cacccuuugg cgacagcuau auaguaaucg gaguagguga aaaaaaaauu     1800

acacaccacu ggcacaggag uggaagcaca auaggaaaag cuuucgaagc aacaguuaga     1860

ggugcaaaaa gaauggcugu acugggagau acagcauggg acuuuggaag uguaggagga     1920

gcacuaaaua gcuuaggaaa aggaauacac caaauauuug gagcagcauu uaaaucacua     1980

uuuggaggua ugaguugguu uucgcaaauu uuaauaggaa cauuauuaau gugguuaggg     2040

cuaaauacaa aaaauggaag uauaucauua guuugucuag cgcucggagg aguacuuaua     2100

uuuuuaagca cagcaguauc agcagacguu ggauguagug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4828
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 1 opt

<400>  4828
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaaaaaaag aagaggaaca       60

gacacaagug uuggaauugu ugguuuacua cuuacgacag cuauggcagu agagguuaca      120

aggagaggua gugcauauua uauguaccua gauagaucag augcaggaga agccauauca      180

uuuccgacca cuuuaggaau gaauaaaugu uauauacaaa uaauggaucu aggacauaug      240

ugugaugcaa caaugaguua cgaaugccca auguuagaug aaggaguaga accugaugau      300

guagauugcu gguguaacac uaccucaaca uggguuguau acggaacaug ucaucauaaa      360

aaaggugaag cuagacgaag cagacgagcu guaacguuac caagucauuc aacacgaaaa      420

cuacaaacua gaucacagac auggcuugaa ucaagagaau auacaaaaca cuuaauuaga      480

guugaaaauu ggauauuuag aaauccagga uuugcacucg cagcagcagc aauugcaugg      540

cuccuuggca guaguacauc acaaaaagua auauauuuag uuaugauauu auuaauagcu      600

ccagcauaca guauucgaug cauaggaguu ucaaacagag acuuuguaga aggaauguca      660

ggaggcacau ggguagacgu aguacuagaa cacggcggau guguuacagu uauggcccaa      720

gauaaaccag ccguagauau ugaauuaguu acuacgacag uaaguaauau ggcagaagug      780

agaucauauu guuaugaagc aaguauauca gauauggcgu cagauagccg uugccccacc      840

caaggcgaag cuuaucuaga caaacaaagu gacacacagu acguauguaa aagaacauua      900

guagacaggg gauggggaaa uggaugcgga cuauuuggca aaggaagcuu aguaacaugc      960

gcaaaauucg cuuguagcaa aaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa     1020

uauagaauua uguugucagu acacgggagu caacauuccg gaaugauagu aaaugacacu     1080

ggucacgaaa cagacgaaaa uagagcaaaa guagaaauaa cgccaaauuc uccuagagca     1140

gaagcaacac uaggaggauu ugggucuuua ggguuagacu gugagccaag aacaggauua     1200

gauuuuucug auuuauauua uuuaacaaug aauaacaaac auugguuagu acauaaagag     1260

ugguuccaug acauaccucu accauggcau gcaggagcag auacuggaac uccacacugg     1320

aauaacaaag aagcacuugu ugaauuuaaa gacgcucaug caaaaagaca aaccguaguu     1380

guauuaggau cacaagaagg ugcggugcac acagcuuuag caggggcccu agaagccgag     1440

auggauggug caaaagguag acuuagcucu ggucaccuua aaugccgacu aaaaauggau     1500

aaauuaagac uaaagggagu aaguuauagc cuauguaccg cagcguuuac auuuacaaaa     1560

auaccagcag aaacacuaca uggaaccgug acaguagaag uucaauacgc aggaacagau     1620

gguccaugua aaguaccagc acaaauggca guagauaugc aaacauuaac accaguggga     1680

agauuaauaa cagcaaaccc aguaauaaca gaaaguacag aaaauucaaa aaugauguua     1740

gaauuagacc ccccuuuugg agauagcuau auagucauag guguuggcga aaaaaaaaua     1800

acacaucauu ggcauagaag cgguucaaca auaggaaagg cauuugaagc aacgguacga     1860

ggugcgaaaa ggauggcugu auuaggagau acggcauggg auuuuggauc aguaggagga     1920

gcguuaaauu cauuaggcaa aggaauacau caaauauucg gagcugcguu uaaaucauua     1980

uuugguggua ugucaugguu cagucaaaua uuaauaggaa cacuuuuagu cuggcuaggu     2040

uuaaacacca aaaaugguag cauaucauua acaugcuuag cauuaggagg aguauuaaua     2100

uuccucagca cagcgguuuc agcagacgua ggauguagug uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4829
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 1 opt

<400>  4829
gggagaaagc uuaccaugag aauaauaaau gccagaaaag aaaaaaaaag aagaggagcg       60

gacacuucag uagguauagu aggacuauua uuaacaacag cgauggccgu agaaguaaca      120

agaagaggaa gugcauauua uauguacuua gauagaagcg acgcaggaga agcgaucagu      180

uucccaacaa cauuagguau gaauaaaugu uauauacaaa uaauggaucu uggacauaug      240

ugugacgcaa cuaugucaua cgaaugucca augcuagacg aaggaguaga accugaugac      300

gucgauuguu gguguaauac aacuagcaca uggguaguau auggaacaug ucaucauaaa      360

aagggugaag cuagaagaag uagaagagca guaacgcuuc caucacacag uacaagaaaa      420

uuacaaacua gaagucaaac auggcuagaa ucuagagaau auacuaaaca ucucauacga      480

guagaaaacu ggauauuucg uaauccggga uuugcauugg cugcggcugu aauagccugg      540

uuauuaggaa guaguacaag ucaaaaagug auauauuuag uaaugauacu acuuauugcu      600

ccagcuuaca guauaagaug caucggagua ucaaauagag auuucgucga aggaaugagu      660

ggagggacau ggguagacgu aguacuagaa cauggaggcu gcguaaccgu aauggcacaa      720

gauaaaccaa caguagauau agaauuagua acaacgacag uuuccaauau ggcugaagua      780

cgaucauacu guuaugaagc cagcauuagu gacauggcau cugauucuag gugucccacu      840

caaggugagg cauaucuaga caaacaauca gauacacaau auguauguaa aagaacauua      900

guagauagag gaugggggaa uggauguggg cuauuuggaa aaggaucguu agucacaugc      960

gcgaaauuug cuuguucaaa aaaaaugaca ggcaaaucaa uucaaccuga gaauuuagaa     1020

uauagaauca uguuaucagu ucauggauca caacacagcg gaaugauagu aaaugauacg     1080

ggacaugaaa cugacgaaaa uagagcaaaa guugaaauaa cuccaaauuc cccuagagca     1140

gaagcaacac uagguggauu uggaucauua ggacuagauu gcgaaccaag aacaggauua     1200

gauuuuagcg aucuauacua uuuaacaaug aauaacaaac auugguuagu acauaaagaa     1260

ugguuucaug auauaccauu accauggcau gcaggggcag acacugguac accgcauugg     1320

aauaauaaag aagccuuagu agaauuuaaa gacgcacaug caaaaagaca aacaguuguc     1380

guauuagguu cucaagaagg agcaguacau acggcuuuag caggagcucu cgaagcugag     1440

auggacggag caaaaggcag acuuucaagu ggacaucuca aauguagguu aaagauggau     1500

aaacuucgau uaaaaggagu aaguuacucc cuauguacug cagcauuuac uuuuacaaaa     1560

auaccagcug aaacuuugca uggaacagua acaguagaag uacaauacgc aggaacggac     1620

ggaccaugca aaguuccagc acaaauggca guagauaugc aaacacugac gccaguagga     1680

agguuaauua cagcgaaccc aguaauuaca gaaucuaccg aaaauucaaa aaugauguua     1740

gaauuagacc ccccuuuugg agacucuuac auaguuaucg gaguagguga aaaaaaaaua     1800

acacaccauu ggcaucguag ugguuccaca auaggaaaag cuuucgaagc aacuguaagg     1860

ggagcaaaaa gaauggcagu uuuaggagac acggcauggg acuucggaag uguaggaggg     1920

gcacugaauu cucuaggaaa aggcauacau caaauauuug gugccgcauu uaaaucauua     1980

uuuggaggca ugaguugguu uucucaaaua uuaauuggua cauuauuagu auggcuagga     2040

uugaauacca aaaacggauc cauaucacua acaugucuag cacuuggagg aguucuaauc     2100

uuucuaucaa cagcuguauc agcagaugua ggcuguucug uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4830
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 1 opt

<400>  4830
gggagaaagc uuaccaugag aauuauuaau gcaagaaaag agaaaaaaag aagaggagca       60

gauacaagug uuggaauagu aggauuacua cuaacaacag caauggcagu ggaaguaaca      120

cgcagaggau cugcauauua uauguaucua gauagaagug augcuggaga agcaauaagu      180

uuuccgacaa cucuaggagu aaacaaaugu uauauucaaa uaauggaucu aggacacaug      240

ugcgaugcaa cuaugucaua cgaguguccu auguuagaug aaggugucga accugaugau      300

guagacuguu gguguaauac aacauccacg uggguagucu auggcacaug ucaucauaaa      360

aaaggagaag cuagacgauc aagaagagca guuacucuac caagccacuc gacaagaaaa      420

cuacaaacaa gaucacaaac auggcuugaa ucaagagaau auacaaaaca ucuaauaaga      480

gucgaaaauu ggauauucag aaauccagga uuugcauuag cagccgcagc aauagcaugg      540

uuauuaggcu caaguacaag ccaaaaagua auauaucuag uaaugauacu cuuaauagca      600

ccagcauacu cuauaagaug cauugguguu aguaacagag acuuugucga aggaaugucg      660

ggaggaacuu ggguagaugu aguacuagaa caugguggau guguaacagu aauggcacaa      720

gauaaaccaa caguagauau agaauuaguu acaacaacgg uaucaaauau ggcagaaguu      780

agaagcuacu guuaugaagc aaguauuuca gacauggcau cagacucaag auguccuaca      840

caaggagaag cauaucuuga uaaacaaagc gauacacagu acguauguaa aagaacacua      900

guugauagag gaugggguaa uggauguggu cuauuuggaa aaggaaguuu aguaacuugu      960

gcaaaauuug cauguucuaa aaaaaugaca ggaaaaucaa uucaaccuga aaacuuagaa     1020

uauagaauaa uguuaucugu acauggaucc caacauucag gaaugauugu aaaugauaca     1080

ggucaugaaa cagaugaaaa uagagcuaaa guagaaauca cuccgaacuc accacgagca     1140

gaagcuacuu uaggaggauu cgggucauua ggccuugauu gcgaaccaag gaccggacua     1200

gauuuuucag auuuauacua cuuaacuaug aauaauaaac acugguuagu acacaaggaa     1260

ugguuucaug acauacccuu accauggcau gcaggagccg acacaggaac uccacauugg     1320

aauaauaaag aagcacuagu agaauucaaa gaugcucaug ccaaaagaca aacaguagua     1380

guuuuaggaa gccaagaagg cgcuguccau acagccuuag caggagcacu cgaagcagaa     1440

auggauggag ccaaaggaag acuaucauca ggucauuuaa aaugucgucu uaaaauggau     1500

aaauuaagac uuaaaggugu uaguuauagc uuauguacag cggcauuuac cuuuacaaaa     1560

auaccugccg aaacacuuca cggaacaguu acaguugaag uacaauaugc agggacagac     1620

ggaccaugca aaguuccugc acaaauggca guugauaugc aaacauuaac accaguaggc     1680

agauuaauaa cagcaaaccc aguuauaacu gaaucaaccg aaaacaguaa aaugauguua     1740

gaauuagauc caccuuuugg cgacaguuau auaguaauag gaguagguga caaaaaaaua     1800

acucaucacu gguacagaag uggaucaacu auagggaaag cauuugaagc aacaguaaga     1860

ggggcaaaaa gaauggccgu auugggagau acggcauggg acuuuggcuc aguaggcggu     1920

gcuuugaaua guuuaggaaa agguauacau caaauauuug gagcagcauu uaaaucauug     1980

uuuggaggaa ugaguugguu uucucaaauu uugauuggua cauuauuagu gugguuagga     2040

cuaaauacca aaaacggaag uaucagccua acuugcuuag cccuaggggg gguacuaaua     2100

uuuuuaagua cagcagugag ugcggacgua ggaugcagug ucgacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4831
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 1 opt

<400>  4831
gggagaaagc uuaccaugag gauaaucaau gcaagaaaag aaaaaaaaag aagaggugcu       60

gacacauccg ucggaauagu aggacuguua cuaaccaccg cgauggcugu agaaguaaca      120

cgacgcggca gcgcauauua uauguacuua gauagaagug augcagguaa agcaaucucc      180

uuuccaacaa cauuaggagu aaauaaaugu uacauacaaa uaauggauuu aggacauaug      240

ugugaugcaa caaugucaua ugaauguccu augcuggaug aaggaguaga accugaugac      300

guugauuguu ggugcaauac cacuucaaca ugggucguau auggaacaug ucaucauaaa      360

aaaggagaag cuagaagaag cagaagagcg guuacucuuc caagucauag uacuagaaaa      420

cuacaaacaa gaucucaaac uuggcuugaa ucaagggaau acaccaaaca uuuaauaaga      480

guagaaaauu ggaucuucag aaauccagga uucgcacuag cagcagcggc aauagcaugg      540

cuacuugguu caucuacaag ccaaaaagua auauauuuag ucaugauacu auuaauagca      600

cccgcauauu cuaucagaug cauaggagua aguaauagag auuuuguaga gggaaugucc      660

ggaggaacau ggguagaugu aguccucgaa caugguggau guguuacagu gauggcacaa      720

gacaagccaa caguagauau agaauuaguu acgacaacug ucucaaauau ggcagaagua      780

agaagcuauu guuaugaagc aagcauaucu gauauggcaa gcgauucaag auguccuacu      840

caaggagaag cguauuuaga uaaacaguca gacacacagu acguauguaa acgaacacua      900

guagacagag gauggggaaa cggaugugga uuauucggaa aagguaguuu aguaaccugu      960

gcaaaauuug cguguucaaa aaaaaugacu ggaaaaagua uacaaccaga aaauuuagaa     1020

uauaggauaa ugcucagcgu gcauggauca caacauucag gaaugauagu aaacgauaua     1080

ggacaugaaa cagacgaaaa ucgagcaaaa gucgagauaa cacccaauuc accacgagca     1140

gaggcuaccc uuggagguuu cggaucauua ggauuggauu gcgaaccacg aacaggacuc     1200

gauuuuagug auuuauauua ucuuacaaug aacaacaaac auugguuagu acauaaagaa     1260

ugguuucaug acauuccacu accauggcau gcaggagcag auacugguac accacacugg     1320

aacaauaaag aagcacuagu agaauuuaaa gaugcucacg cuaaaagaca aacaguagug     1380

guauuaggaa gucaagaagg agcaguacau acagcacuag caggagcccu ggaagccgaa     1440

auggauggag caaaaggaag gcuaucauca ggccaucuaa aaugcagacu aaaaauggau     1500

aaauugagau uaaaaggagu aucguauagc uuaugcacag cagcguuuac uuuuacaaaa     1560

auaccagcug aaacacuaca cggaaccgua acuguagagg uacaauacgc aggaacagau     1620

gguccaugua aagucccagc acaaauggca guagauaugc aaacauuaac acccguagga     1680

aggcuuauaa cugcaaaucc aguuauaaca gaaaguacag aaaauucuaa aaugaugcug     1740

gaauuagauc cacccuucgg agauucauac auaguaauag gaguaggaga uaaaaaaauu     1800

acacaucauu ggcauagauc ugggaguaca auugguaaag ccuuugaagc aacagugcga     1860

ggcgcaaaaa gaauggcagu auuaggagau acagcuuggg auuuuggauc agugggagga     1920

gcauuaaaua guuuaggaaa aggaauacau caaauauucg gagcagcauu uaaaagucua     1980

uuuggaggca ugucaugguu uucacaaauu cuuauaggaa cgcuuuuagu augguuagga     2040

uuaaauacaa aaaauggauc aauaucacug acauguuuag cgcuaggugg cguuuuaaua     2100

uuuuuaagca cggcaguauc agcagacgua ggaugcucag uagauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4832
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 1 opt

<400>  4832
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaagaaaaag aagaggagca       60

gauacaagca uagguauagu agggcuauua uuaacaacug cuauggcagc agaaaucacg      120

agaagaggaa gugcauauua cauguauuug gacagaucug augcaggaaa agcaauaucu      180

uucgcaacaa cuuuaggcgu aaacaaaugu cauguucaaa uaauggacuu aggccacaug      240

ugugaugcga caaugaguua ugaaugucca auguuagaug aaggaguaga gccagaugau      300

guugauuguu ggugcaauac aacaagcaca ugggucguuu acgggacuug ucaucauaag      360

aaaggagaag caagaagaag uagacgagcu guuacuuuac caagccauag uacaagaaaa      420

uugcagacaa gaagccaaac auggcucgaa agcagagaau auacaaaaca uuuaauaaaa      480

guagaaaauu ggauauuccg aaacccagga uuuacgcugg uagcaguagc cauagcuugg      540

uuguuaggau ccuccacguc acaaaaagug auauacuuag ugaugauauu acuaauugca      600

ccagcuuauu caauaaggug uauaggagua ucaaauagag auuuuguaga aggaaugagu      660

ggaggaacuu ggguagacgu aguacuagaa cauggagguu gugugacugu aauggcacaa      720

gauaaaccga cuguugauau agaacuugua acaacaacug uaucaaauau ggcugaagua      780

agaucauacu gcuaugaagc aucuauaagu gauauggccu ccgauucaag gugcccaaca      840

caaggggagg cuuacuuaga uaaacaaagu gauacacaau auguauguaa acgaacauua      900

guagauagag gaugggguaa ugguugcgga uuauuuggaa aaggaaguuu gguaacaugu      960

gcuaaauuua cuugcaguaa gaaaaugaca ggcaaaagca uacaaccgga aaaucuggaa     1020

uauagaauaa uguuaucagu acauggauca caacauagug gaaugauagu aaaugacacu     1080

gggcaugaaa cagaugaaaa uagagcaaaa guagaaguua caccgaacag cccaagagcg     1140

gaagcaacuu uagguggguu cggaucccua ggacuagacu gugaaccaag aacaggcuug     1200

gauuuuagug acuuauauua ccuaacaaug aacaauaaac auuggcuagu ucauaaagaa     1260

ugguuucacg auauaccauu accauggcac gcaggagcag auacaggaac accacauugg     1320

aauaauaaag aagcgcuagu cgaguucaaa gaugcacacg caaaacgaca aacaguaguc     1380

guacuugguu cacaagaagg agcaguucac acugcuuuag cgggugcauu agaagcagaa     1440

auggauggag caaaaggaag acuauuuuca ggacaucuua aaugccgucu uaaaauggau     1500

aaauuaagac uuaaaggagu aaguuauuca cuaugcacag cagcauucac auuuacuaaa     1560

guuccugcag aaacauuaca cggaacagug acaguugaag uccaauacgc uggaacagau     1620

gguccaugua aggucccagc acaaauggcg guagauaugc aaacauugac accaguugga     1680

agacuaauua cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugauguua     1740

gaauuggacc cacccuuugg agauaguuau auaguuauag gaguaggaga uaagaaaaua     1800

acacaucacu ggcauagauc uggaucaacg auaggaaaag cauuugaagc aacuguaaga     1860

ggagcuaaaa gaauggcagu acuuggagau acagcauggg acuucggcuc aguaggcggu     1920

gucuucaauu cauuaggaaa aggaauacau caaauauuug gcgcagcauu uaaaucacua     1980

uucggaggua ugaguugguu uucacaaaua cuuauaggaa cccuuuuagu uuggcuagga     2040

cuaaauacua aaaaugguuc aaucaguuua acauguuugg cauuaggagg aguuaugauc     2100

uuucuaagca cagcuguuag ugcugaugua ggguguagcg uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4833
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 1 opt

<400>  4833
gggagaaagc uuaccaugag aauuauaaau gcaagaaaag agagaaagag acgaggagcc       60

gacacaucua uaggaauaau aggacuacua uuaacaaccg caauggcagc agaaauaacu      120

cgaagaggga gugcauacua cauguauuua gaucgaucag acgcugguaa agccauaagu      180

uuugcgacua auuuaggagu aaacaaaugc cauguucaaa uuauggaccu aggacauaug      240

ugcgaugcaa caaugaguua cgaaugucca augcuagaug aaggaguaga accagacgau      300

guagauugcu gguguaauac aacaucaaca uggguuguau acggaacaug ucaucauaaa      360

aaaggagaag caagacgauc aagaagagca guaacuuugc ccucucacag cacaagaaaa      420

uuacagaccc gaagccaaac cuggcuagaa ucaagagaau auacaaaaca ucuuauaaaa      480

guugaaaauu ggauauuuag aaaucccggu uuugcguuag cagcaguagc uauagccugg      540

uuacuuggaa gcaguacauc acaaaaaguu auauauuuag uuaugauauu auuaauugcg      600

ccagcuuauu caauuagaug uaucggagua agcaaccggg auuucguuga gggaauguca      660

ggggguacau ggguagaugu aguauuagaa caugggggau gcguaacagu aauggcgcaa      720

gauaaaccaa caguagacau agaauuagua acaacgaccg ugucaaacau ggcagaaguu      780

cgcucauauu guuaugaagc aaguauaucu gauauggcau cagacucaag auguccuaca      840

caaggugaag cauacuuaga caaacaaucu gauacucagu acgucuguaa acgaacucua      900

guagacagag gauggggaaa uggaugcggu cuauuuggua aaggaucacu agugacuugu      960

gcaaaauuua cauguagcaa aaaaaugaca gguaaaagua uucagcccga aaaccuugag     1020

uauagaauaa uguuaucagu ccauggaucu caacauagug guaugauagu caaugauaua     1080

ggccaugaaa cagaugaaaa uagagcaaaa guugaaguaa caccaaauuc accaagagca     1140

gaagcaaccc uagggggauu uggaucacuu ggauuagauu gugaaccuag aacuggacua     1200

gacuuuucag acuuauacua uuuaacaaug aacaauaaac auugguuggu acauaaagaa     1260

ugguuucacg auaucccuuu acccuggcau gccggagccg auacaggaac cccacauugg     1320

aauaauaaag aggcacuagu ugaauuuaag gaugcacaug cgaaaagaca aacaguagua     1380

guacuaggau cacaagaggg agccguacau acggccuuag cuggagcacu agaagcagaa     1440

auggacggag cuaaaggaag acuauuuagc gggcacuuga aaugccgauu aaaaauggau     1500

aaauuacgac uuaaaggagu uuccuacuca uuaugcacag cugcauuuac guuuacuaaa     1560

guaccugcag aaacauugca cggaacagua acaguagaag uacaauaugc ugguacagau     1620

ggacccugua aagugccagc gcaaauggcu guggauaugc aaacauuaac uccuguugga     1680

agacuaauaa cagcaaaucc aguaauaaca gaaucaacug aaaacaguaa aaugauguua     1740

gaauuagacc cuccauuugg ggauucguau auaguaauag guguuggaga uaaaaaaaua     1800

acacaucauu ggcauagaag ugggucgaca auaggaaaag cuuucgaagc aacaguaaga     1860

ggagcaaaaa gaauggcagu cuuaggagac acagcauggg auuuuggauc aguuggaggu     1920

gucuucaaua guuuagguaa aggaguacac caaaucuucg gagcagcuuu caaaagucua     1980

uuugggggaa ugucaugguu uucacaaaua cuuauuggaa cauuacuagu cugguuagga     2040

cucaauacaa aaaauggcag uauaagcuua acauguuugg cauuaggagg cgugaugauu     2100

uuccuaagca cagcaguauc agcagacgua gguuguagug uugauuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4834
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 1 opt

<400>  4834
gggagaaagc uuaccaugcg aauaauaaac gcaagaaaag aacgaaaaag aagaggagca       60

gacacaucaa uuggaauaau aggucuguua uuaacaacag ccauggccgc agaaauuaca      120

agaagaggca gugcauauua uauguauuua gaccgaagug augcuggaaa agcaauauca      180

uucgcuacua cauugggagu caauaaaugu cauguccaaa uuauggaccu uggucauaug      240

ugcgacgcaa cuaugucaua ugaauguccg auguuagaug aggguguuga accagacgac      300

guagauuguu ggugcaacac aacaaguacu uggguaguau auggaacaug ccaucauaaa      360

aaaggagagg cacgccgaag uagaagagca guaacacuac caucucacuc aacaagaaaa      420

uuacaaacua ggucacaaac augguuagag ucacgcgaau acacaaaaca uuuaauaaaa      480

guagaaaauu ggauauuuag aaauccagga uuugcacuug uagccguagc cauagcuugg      540

cuacuuggua gcucaacaag ucaaaaagua auauauuuag uaaugauauu acuaauagcc      600

ccagcauaca guauaaggug cauaggugua ucaaauagag auuuuguaga agguauguca      660

ggcggaacau ggguagaugu aguacuugaa cacggaggau gcguaacagu aauggcgcaa      720

gacaaaccca caguagacau ugaacuagua acaaccacag uaagcaauau ggcugaagua      780

agaucuuauu guuacgaagc aaguauaagc gauauggcaa gugauucaag auguccuaca      840

caaggcgaag cauauuugga uaagcaaagu gacacucaau auguuuguaa aagaacuuua      900

gucgacagag gauggggaaa uggaugcgga cuuuuuggaa aaggaagccu aguaacaugc      960

gccaaauuca cauguaguaa aaaaaugaca ggaaaaagua uacaaccuga aaaccuagag     1020

uacagaauaa ugcuuagugu acacggaagc caacacuccg gaaugauugu aaaugauauu     1080

ggucaugaaa cagacgaaaa uagagcaaaa guagaaguua ccccaaacuc accuagagca     1140

gaggcaacac uagguggauu uggcucauua ggauuagauu gugaacccag aacagggcua     1200

gacuuuagug acuuauauua ucuaacgaug aacaauaaac auugguuagu ccauaaagag     1260

ugguuccaug auauaccccu accauggcau gcgggagcag auacugguac accacauugg     1320

aacaacaaag aagcauuagu agaauuuaaa gacgcucaug cuaaaagaca aacuguagua     1380

guauuaggau cacaagaagg ugcagugcac acagcauuag caggugcucu agaagcagaa     1440

auggauggag caaaaggaag auuauucuca gggcaucuaa aaugccgauu aaaaauggau     1500

aaauuaagau uaaaaggggu aaguuauuca uuauguacag cagcauucac uuuuacuaaa     1560

guaccagcag aaacauuaca cgguacaguc acaguagaag uacaauaugc aggaacagau     1620

ggaccaugca aaguaccagc acagauggca guagauaugc aaacauuaac accaguugga     1680

cguuuaauaa cagcgaaucc aguaauaaca gaaucaacag aaaauaguaa aaugaugcua     1740

gaguuggauc cgccauuugg ggauucuuac auaguuauag gaguggguga caaaaaaaua     1800

acucaccauu ggcacagaag cgguucaaca auaggaaaag cauuugaagc aacaguaaga     1860

ggagccaaac gaauggcagu acucggagau acagcauggg auuuuggauc agucggaggu     1920

guauuuaacu cauuaggaaa agguauacau cagauauucg gggccgcuuu uaaaucauua     1980

uuuggcggaa ugucaugguu cagucaaaua uuaauuggca cuuuauuggu uuggcuagga     2040

cuuaauacaa aaaaugggag uauaagccuu accuguuuag cguuaggagg aguuaugaua     2100

uuuuugucaa ccgcaguaag ugcggaugua ggaugcagug uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4835
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 1 opt

<400>  4835
gggagaaagc uuaccaugag gauuauaaac gcaaggaaag agagaaaaag acguggagcc       60

gauacaagca ucggaauaau aggccuauua cuaacaacag ccauggcagc ugagauuaca      120

agaagaggaa gcgcauauua cauguauuua gauagaagug augcuggaaa agcaauauca      180

uuugcaacaa auuuaggagu aaauaaaugu cauguccaaa uaauggacuu agggcacaug      240

ugcgaugcaa caaugagcua ugaaugucca auguuagaug aaggagucga accagaugac      300

guagauuguu gguguaauac aacaucaaca uggguaguau acgguacuug ccaucauaag      360

aaaggagaag cgagaagauc aagacgagcc gugacucucc cgagucacag uacacguaaa      420

uuacaaacuc gaucacaaac augguuagaa ucucgagaau auacaaaaca uuuaaucaaa      480

guagaaaacu ggauauucag aaauccagga uucgcacuag ccgcaguagc aauagcaugg      540

uuauuaggga guaguacuuc ucaaaaagua aucuaucucg uaaugauccu auuaauugca      600

ccagcauaua gcauacguug cauaggagua agcaacaggg auuuugugga agguaugagu      660

ggaggaacau ggguagaugu aguauuagaa cacggagguu gcguaacugu aauggcccaa      720

gacaaaccaa caguagauau agaacucgua acaacaacag uaucaaauau ggcagaagua      780

agaaguuauu guuaugaagc caguauuuca gauauggcau cagacuccag augcccgaca      840

caaggggaag ccuauuuaga caagcaaagu gauacacaau auguauguaa acgaacauua      900

guugauaggg gaugggguaa uggaugugga uuauucggaa aaggaucauu aguaacaugu      960

gcaaaauuca cuuguuccaa gaaaaugacc ggaaaaucaa uacaacccga aaacuuagaa     1020

uacagaauaa ugcuaucugu acauggauca caacacagug gaaugauagu aaaugacgaa     1080

aauagggcaa aaguagaagu cacaccaaac agcccucgag cggaagcaac auuaggagga     1140

uuuggaaguc uaggauuaga cugugaacca aggacugguc uagauuuuuc ggaccuauau     1200

uauuuaacca ugaauaacaa acauugguua guacauaaag aaugguuuca ugauauucca     1260

uuaccguggc augcuggugc agauacagga acaccgcauu ggaacaauaa agaagcauua     1320

guagaauuua aagaugcaca ugcaaaaaga caaacaguag uaguuuuggg cagccaagaa     1380

ggugcaguuc auacugcacu ggcuggagcu cucgaagcag aaauggaugg agccaaagga     1440

aggcuauuuu caggacacuu gaaauguagg cuaaaaaugg auaaacuaag auuaaaaggc     1500

guaucauauu cucuauguac agcggccuuu accuuuacaa aaguuccagc agaaacucua     1560

cauggcacag uuaccguaga aguacaauau gcaggcacag auggaccaug caaaguacca     1620

gcacaaaugg caguagacau gcaaacauua acacccguag ggagauuaau aacagcaaau     1680

ccaguuauaa cugaaucaac ugaaaacagc aaaaugaugu uggaacuaga uccaccuuuc     1740

ggcgauaguu acauaguaau agguguagga gauaagaaaa uaacacauca cuggcaucgu     1800

aguggaagca caauaggaaa agcauuugaa gcaacaguaa gaggggcuaa aagaauggca     1860

guacuaggag auacagcuug ggacuucgga ucaguagggg guguauuuaa uagcuuagga     1920

aaaggcauac accaaauauu uggagcagca uuuaaaaguu uauuuggggg aaugaguugg     1980

uucucacaaa uauuaauagg aacuuuauug guuugguuag gucucaacac uaaaaacggu     2040

aguauaucau uaacaugucu agcacuagga ggaguaauga ucuuuuuauc aacagcagua     2100

ucagcagacg uagguuguag uguagauuuu ucaaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4836
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 1 opt

<400>  4836
gggagaaagc uuaccaugcg aauaauuaau gcaagaaaag aaagaaaacg uaggggagca       60

gacacaucaa uuggcauugu cggauuauua uuaacaacug cuauggcagc agaaaucaca      120

agaagaggua gugcuuauua uauguauuua gauaggagug augcaggaaa agcaauauca      180

uuugcaacca cacuaggcgu aaauaaaugu caugugcaga uaauggauuu aggacauaug      240

ugcgaugcua cuaugaguua ugaaugucca auguuagaug aaggaguaga acccgaugau      300

guagacuguu gguguaacac aacgaguaca ugggugguau auggaacaug ucaucauaag      360

aaaggagaag caagaaggag uagaagggcc guuacacuac cuagucauag uacaagaaaa      420

uuacaaacaa gaagccaaac augguuggaa agccgcgaau acacaaaaca uuuaauaaaa      480

guagaaaauu ggauuuucag aaauccgggc uuugcccuag uagcaguagc aauugcaugg      540

uuacuaggaa guuccacaag ucaaaaagua auuuauuuag uaaugauauu acuaauagcg      600

ccagcauacu cuauaaggug uauuggagua aguaauagag auuuuguaga aggcaugagu      660

ggaggaacuu ggguagaugu aguacuagaa cauggaggau gugucacagu gauggcccaa      720

gacaaaccca caguagauau agagcuagua acaacuaccg uaaguaacau ggcggaagua      780

agaucauacu guuaugaagc aaguauauca gacauggcuu ccgauaguag auguccgaca      840

caaggagaag cguaccugga uaaacaaagc gacacacaau auguauguaa aagaaccuua      900

guagauagag gauggggaaa ugguuguggu uuauuuggaa aggguagcuu aguaacaugu      960

gcaaaauuua cauguucaaa gaaaaugacg ggaaaaucaa uacaaccuga aaacuuggaa     1020

uauagaauaa ugcuaagugu acauggauca caacauagug gaaugauagu aaaugauaca     1080

ggacaugaaa cggaugaaaa ucgagcaaaa guggaaguaa cgccaaacuc accaagagcg     1140

gaagcgacau uaggagguuu cggaucauua ggguuggacu gugaaccacg aacaggacua     1200

gauuucagug auuuauacua uuuaacuaug aauaacaaac auugguuagu ucauaaagaa     1260

ugguuucaug auauaccauu accauggcac gcaggcgcag auacgggaac accacauugg     1320

aacaauaaag aagccuuagu cgaauuuaaa gaugcacacg cgaaacgcca aacuguagua     1380

guuuuaggau cacaagaagg agcaguacau acagcauuag cuggugcauu ggaagcggaa     1440

auggauggag caaaaggaag auuauuuagc ggucauuuaa aaugucgacu aaaaauggau     1500

aaauuaagau uaaagggagu aucauauuca cuauguacag cagcauuuac uuuuaccaaa     1560

guuccagcug aaacauuaca uggaacggua acuguagaag uacaauaugc aggaacugau     1620

ggaccuugua aaguaccugc ucagauggca guugauaugc aaacauuaac accuguagga     1680

cgauugauaa ccgcaaaucc agugauaaca gaaucuacag aaaauaguaa aaugauguug     1740

gaauuagauc caccuuuugg ugacaguuau auuguaauag gaguagguga uaagaaaauu     1800

acacaccacu ggcacaggag ugguaguacu auaggaaaag ccuuugaagc uacaguaaga     1860

ggagcaaaaa gaauggcugu guuaggagau acagcuuggg acuuugguuc aguuggcgga     1920

guuuuuaauu cguuagggaa aggcauucau caaauauucg gugcagcauu caaaagccua     1980

uucggaggaa ugagcugguu cagucaaaua cuuauaggaa cacuauuagu guggcuuggu     2040

uuaaauacga aaaauggauc aauaaguuua acauguuuag cauuaggugg uguaaugaua     2100

uucuuaagua cagcaguaag cgcagaugua ggauguucug uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4837
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 1 opt

<400>  4837
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag agcgaaaaag aagaggagca       60

gacacaucua uugggauagu aggacuauua uuaacaacag cgauggcggc ugaaauuaca      120

agaagaggaa gugcuuacua uauguacuua gaccgaagug augcuggcaa agcaauaucg      180

uucgcuacaa cacuuggggu aaacaaaugc cacguucaaa uaauggacuu aggacauaug      240

ugugaugcua cgaugagcua cgaaugucca auguuagaug aagguguaga accagaugac      300

guagacuguu gguguaacac aacaaguaca uggguaguuu auggaacaug ucaacacaag      360

aaaggggaag caagacguag cagacgagca gugacacuac cuagucauag uacuagaaaa      420

cuacaaacaa gaucacagac cugguuagaa aguagagaau auacaaaaca ucuaauaaaa      480

guagaaaauu ggauauuuag aaaucccgga uuugcccuag uugcaguagc aauagcaugg      540

uuauuaggau caagcacaag ucaaaaagua auauauuuag uaaugauauu acuuauagca      600

ccagcuuaca gcauuagaug uauaggugua ucaaauagag acuuugugga ggguaugagc      660

ggaggaacau ggguugaugu aguauuagaa cauggaggcu guguuacugu aauggcucaa      720

gacaaaccca caguagauau agaauuagua acgacaacag uaucgaauau ggcagaaguc      780

agaucuuauu guuacgaagc gagcauauca gacauggcaa gugauaguag augucccacu      840

caaggugaag cauauuuaga caaacaauca gauacacaau auguauguaa aaggacauua      900

guagaccggg guuggggaaa uggaugcgga cuauuuggua aaggcagcuu aguuacaugu      960

gccaaauuua cauguucuaa gaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa     1020

uauagaauua uguuaagugu acauggaagc caacauucag gaaugaucgu aaaugacauu     1080

ggacaugaga cugaugaaaa uagagcaaaa guagagguaa ccccaaauuc uccuagggca     1140

gaagcaacac ucggaggauu cggaaguuua ggacuagauu gugaaccaag aacaggacua     1200

gauuuuucag auuuauacua ucuaacaaug aauaauaaac auuggcuagu gcauaaagaa     1260

ugguuucacg acauuccgcu accauggcau gcaggagcag auaccggaac accacauugg     1320

aauaacaaag aagcucuggu agaauuuaaa gaugcacaug caaaaaggca aacgguagua     1380

guucuuggau cacaagaagg agcaguacac acggcauuag caggagcauu agaggcugaa     1440

auggauggug cuaaaggucg gcuauuuuca ggucauuuaa aaugcagguu aaaaauggau     1500

aaauugagau uaaaaggagu aagcuauagu cuauguacag cuguuugcac agcugcaaaa     1560

guaccagcag aaacauuaca uggaacugua acaguagaag uacaauacgc aggaacagau     1620

ggaccuugua aaguaccagc acaaauggcu guagauaugc aaacacuuac cccaguagga     1680

cgauuaauua cagcaaaccc cguaauaaca gagucaacgg aaaauaguaa aaugauguua     1740

gaauuagacc cucccuuugg ugauucauac auaguaauag gaguaggaga uaagaaaaua     1800

acacaccauu ggcacagauc aggcucaaca aucggaaaag ccuucgaagc uacaguacga     1860

ggagcaaaaa gaauggcagu auuaggagau acugcauggg auuuuggcag uguaggagga     1920

guauuuaaua guuuagguaa agguauucau caaauuuuug gagcagcuuu caaaucauua     1980

uucggaggaa ugaguugguu uucacaaaua cuuauaggaa cacuauuagu augguuagga     2040

cuuaauacga aaaacggguc aaucagcuua acauguuuag cacuaggugg cguaaugaua     2100

uuucuuucaa cagccguuag ugcagaugua ggcuguuccg uugauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4838
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 1 opt

<400>  4838
gggagaaagc uuaccaugag aauaauaaac gcuagaaaag aacggaagag aagaggagcu       60

gaugcgucaa uaggaauagu aaguuuauua uuaacaacag uaauggcugc agaaauuaca      120

aggagaggau cagcuuauua uauguauuua gacagaagug augcgggaaa agcaauaagu      180

uuuguaacaa cauuaggagu aaauaaaugu cauguccaaa uaauggacuu ggggcauaug      240

ugcgaugcaa caaugucuua ugagugccca auguuggaug aaggaguuga accagaugau      300

guagauuguu gguguaauac aacaucaacu uggguaguau acggaacaug ccaccacaag      360

aaaggggaag cuagaagauc aagaagggca guaacauugc caagucauag uacgagaaaa      420

cuacaaacac guagccagac auggcuugaa ucaagagaau auacaaaaca cuugauaaaa      480

guagagaauu ggauuuuccg aaauccaggu uucgccuuag uugcaguagc aauugcaugg      540

uuacuaggaa gcagcacuuc ucaaaaagua auauauuuag uaaugauauu acuaauagca      600

ccagcauauu caauaagaug uauaggagug aguaacagag auuucguaga agguaugagu      660

ggaggaacau ggguagacgu aguacucgaa cacgguggau gcguaacagu aauggcucaa      720

gacaaaccaa caguagauau ugaacucgua acaacaacag uaucaaauau ggccgaaguu      780

aggucuuauu gcuaugaggc aucaauauca gacauggcau cagauagcag auguccuaca      840

caaggagaag cauauuuaga uaaacaauca gacacacaau acguaugcaa aagaacauua      900

guagauagag gcuggggaaa cggcugugga cuguuuggga aaggaucucu cguaacaugc      960

gcuaaauuca ccuguaguaa gaaaaugaca ggaaaaagua uacaaccaga aaaccuagaa     1020

uauagaauua uguuaagcgu gcauggaagc caacacagcg ggaugauagu uaaugaugaa     1080

aauagggcaa aaguagaggu cacgccaaac agcccaagag cagaagcaac auuaggaggu     1140

uucggaagcc ucggauuaga uugugaacca aggacuggau uagauuucuc agauuuauau     1200

uaucuuacaa ugaauaacaa acacuggcua guacauaaag aaugguuuca cgauauucca     1260

uuaccauggc auucaggagc agacacugaa acaccacacu ggaauaauaa agaagcguug     1320

guagaauuua aagaugcaca cgcaaaaaga caaaccguag uagucuuagg uucgcaagaa     1380

ggagcaguac auacagcacu agcaggcgcg cuagaagcug aaauggaugg ugcaaaagga     1440

agacugucau caggucaucu aaaauguaga uuaaaaaugg auaaauuaag acuaaaaggu     1500

guaucauauu ccuuauguac cgcagcguuu acuuucacaa aaguuccagc cgaaacauua     1560

cauggaacag uaacuguaga aguacaauau gcagggcgag acggaccuug uaaaguucca     1620

gcacaaaugg caguugauau gcaaacauua acaccaguug ggagauuaau aaccgcaaac     1680

ccaguaauaa cugaaucaac cgaaaauuca aagaugaugu uagaauuaga ucccccauuu     1740

ggagacuccu auauagugau aggaguugga gauaagaaga uaacccauca uuggcauaga     1800

ucaggaagca uuauaggaaa agcauuugaa gcaacaguaa gaggcgcaaa aagaauggca     1860

guacuaggcg auacggcaug ggacuuugga ucagugggag gaguuuucaa cagucuagga     1920

aaagguauuc accaaauauu cggagccgca uucaaaagcc uauucggagg uaugucaugg     1980

uuuucacaga uucuaauagg aacgcuucua guaugguugg gauuaaauac uaaaaaugga     2040

aguauaucac uaacaugucu ugcuuuagga ggaguaauga uauucuuguc aacagcaguu     2100

ucugcagacg uuggaugcag uguggauuuu aguaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4839
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 1 opt

<400>  4839
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaagaaaaag aagaggagcu       60

gauacaucua uuggaauagu aggcuuguua uuaacaacag cuauggcagc agaaauaaca      120

agaagaggaa gcgcuuacua uauguauuua gacaggagug augccggaaa agcaauauca      180

uuugcaacaa cauuaggggu caauaaaugu cauguccaaa uaauggacuu aggacauaug      240

ugcgacgcaa caaugaguua ugagugucca auguuggacg aaggaguaga accagaugau      300

guagauuguu gguguaauac gacuuccaca uggguaguuu acggaacuug ucaucauaag      360

aaaggagaag cuagaagaag caggagagca guaacacuac caucacauag uacuagaaaa      420

cuucaaacaa gaagccaaac auggcuagag ucaagagaau auacaaaaca cuugauaaaa      480

guagaaaauu ggauauuuag aaauccaggc uuugcauuag uggcaguagc uauagccugg      540

cuuuuaggaa gcaguacaag ucaaaaagua auauauuuag uaaugauauu auuaauagca      600

ccagcauauu caaucagaug caucggagug aguaauagag acuucguaga agguaugagc      660

ggaggaacau ggguagaugu gguacuagaa cacggaggcu guguaacagu aauggcacaa      720

gauaagccga ccguagauau ugaauuagua accaccaccg ugaguaauau ggcagaaguu      780

agaucauauu gcuaugaagc aaguaucagu gauauggcaa gcgauaguag auguccgaca      840

caaggagaag cauaccuaga uaaacaaagc gacacacaau acguauguaa aagaacacuc      900

guagauagag gauggggaaa cggaugugga uuauuuggga aaggaucccu aguaacaugu      960

gccaaauuua cuuguucgaa gaaaaugacu ggaaaaagua uacaaccuga aaacuuagaa     1020

uauagaauaa ugcuaucagu acaugguagc caacauagcg gaaugauagu aaaugaugaa     1080

aacagggcaa aaguagaagu aacgccuaau agcccaagag cagaagcuac acuaggugga     1140

uuuggaaguu uaggacuaga uugcgaacca cgaacggggu uagauuuuag ugacuuauau     1200

uauuuaacua ugaacaauaa acauugguua guacacaaag aaugguuuca cgacauacca     1260

uuacccuggc augcaggagc ggauacagga acaccacauu ggaauaacaa agaagcucua     1320

guggaauuua aagaugcaca ugcaaaaagg caaacaguug uaguacuggg aucacaagaa     1380

ggagcgguuc auacagcccu agcaggagca cuagaagccg aaauggaugg agcgaaagga     1440

agauuauucu caggacauuu aaaaugucga uuaaaaaugg acaaacuacg auuaaaagga     1500

guuaguuacu cauuauguac agcagccuuc acguuuacaa aaguaccagc agaaacacua     1560

cacggaacag uaacuguaga aguacaauac gcuggaacag auggaccuug uaaaguaccu     1620

gcccaaaugg cgguagauau gcaaacauua acaccugugg guagguuaau aacagcaaau     1680

ccaguaauaa cugaaucaac agaaaacagu aaaaugaugu uagaauuaga uccacccuuu     1740

ggugauagcu auauagucau aggaguaggu gacaagaaaa ucacacauca uuggcaccga     1800

aguggcucaa cgauagguaa agcauucgaa gcaacaguuc gaggagcuaa aagaauggca     1860

guacuaggag auacagccug ggauuuuggg aguguaggug gaguauucaa uagccuaggg     1920

aaaggaauac accaaauauu cggggcagca uucaaaaguc uauuuggugg uaugaguugg     1980

uuuucacaaa uauuaauagg cacuuuauua guauggcuag gguuaaauac uaaaaacgga     2040

agcauuagcu uaacaugccu agcgcuggga gguguaauga uauuucuaag uacagcagua     2100

agugcagaug uaggauguag cguugauuuu ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4840
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 1 opt

<400>  4840
gggagaaagc uuaccaugag aauuauaaau gcaagaaaag aaagaaaaag aaggggagcu       60

gacacuucaa uaggaauaau aggauuacua cuuaccacag caauggcagc agaaauaaca      120

cgaagaggau cugcauauua cauguaucuc gauagaagcg acgcagguaa agccauaagu      180

uuugcaacca cauuaggugu aaauaagugu cauguacaaa ucauggacuu aggacauaug      240

ugugacgcaa ccaugagcua ugaaugcccu augcuagacg aaggaguaga acccgaugac      300

gucgacuguu ggugcaacac uacaagcaca ugggucguuu augguaccug ucaucauaag      360

aaaggagaaa cucgaagauc aagaagaagu guaucucuua gauaucauua cacaagaaaa      420

cuacagacaa gaucucaaac auggcuagaa ucaagagaau auaagaaaca ucuaauaaug      480

guugaaaauu ggauauucag gaacccagga uuugcaauag uuaguguugc aauaacaugg      540

cuuaugggaa guuuaacaag ccaaaaaguu auauacuuag uaaugauagu ucuaauugua      600

ccagcuuauu caauaagcug cauaggagug aguaacagag auuuagucga aggaauguca      660

gguggaacau ggguagaugu aguauuagaa caugggggau gcguuacaga aauggcccaa      720

gauaaaccga caguagauau cgaauuagua acaaugaccg uaucaaacau ggccgaagua      780

agaucauauu guuaugaagc aucacuauca gauauggcaa gugcaagcag augcccaacu      840

caaggggaac caucucuaga uaaacaauca gacacacaau caguaugcaa aagaacauug      900

ggugauagag gauggggcaa cggcuguggc auuuuuggaa aaggaagucu aguuaccugu      960

aguaaauuca cgugcuguaa gaaaaugcca ggaaaaagua uucaaccaga aaaccuugaa     1020

uacagaauua uguuaccagu acauggaucg caacauagug gcaugauagu gaaugauaua     1080

ggucacgaaa cagacgaaaa uagagcaaaa guugaaguga caccaaacag uccaagagca     1140

gaagcaacau uaggaggauu uggaucauua ggauuagauu gugaaccacg aaccgggcua     1200

gauuucagcg aucuuuauua cuuaacgaug aauaauaaac acuggcuagu acauaaagaa     1260

ugguuccaug acauaccauu accauggcac gcaggagcag auacuggaac uccacauugg     1320

aauaauaaag aagcacuugu ugaguucaaa gaugcacaug caaaaaggca aacaguagua     1380

guacuaggaa gccaagaagg agcuguucac acagcguuag caggcgcacu ugaagcugaa     1440

auggauggag caaaaggaag auuguuuuca ggacauuuaa agugcagacu uaaaauggac     1500

aaacuaagac uaaaaggcgu cucuuacuca uuauguacag cagcauuuac auucacuaaa     1560

gugccagcag aaacacuaca uggaacuguc acaguagaag uccaaucugc agguacagau     1620

ggcccaugua aaguacccgc ccaaauggca guugacaugc aaacgcugac accaguaggu     1680

agacuaauaa cagcaaaccc uguaaucaca gaaucaacag aaaacuccaa aaugauguua     1740

gaacuagauc caccuuucgg agauaguuau auuguaauug gaguaggaga uaagaaaaua     1800

acacaucauu ggcauagaag cggaaguacu auaggaaaag ccuucgaagc uacaguaaga     1860

ggcgcuaaaa ggauggcugu cuuaggugau acagcuuggg auuuuggauc aguagggggu     1920

guauucaaua gucuaggaaa aggaauacau caaauuuuug gagccgcguu caaaaguuua     1980

uucggaggua ugucgugguu uucacaaauc uuaauaggaa cauuacuggu auggcuagga     2040

cuaaauacua aaaauggcag uauuagcuua acauguuuag cuuuaggagg aguaaugaua     2100

uuucuuagca cagcaguuag ugcggauguc ggauguucug uagacuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4841
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 1 opt

<400>  4841
gggagaaagc uuaccaugag aauuauuaac gcucguaaag aaagaaaaag acguggugca       60

gacacaagca uagggauaau agguuugcua uuaacaacug caauggcugc ugaaauaaca      120

agacgaggau cagcauauua uauguaccug gauaggucug augcaggaaa agcaauauca      180

uucgcaacua caaugggagu aaauaaaugu cauguacaaa uaauggauuu aggacacaug      240

ugugaugcua caaugaguua ugaauguccu auguuagacg aaggaguaga accugaugau      300

guugacuguu gguguaauac uacuagcaca uggguuguau augggaccug ccaucauaag      360

aaaggagaag caaggccauc aagacgagca gucacauuac cgagucauag uacucgaaaa      420

uuacaaacuc gaagucaaac gugguuagaa ucacgugaau acacaaaaca cuuaauaaaa      480

guagaaaauu ggauacuucg aaacccuggg uuuacgguag cagccuuagc aauggcaugg      540

cuucuaggaa auaguacaag ucaaagggua auauaccuag uaaugauuuu auuaauagca      600

cccgcauauu caauaagaug cauaggagua agcaauagag auuuuguaga agguauguca      660

ggaggaacau ggguagaugu uguacuagaa cacggcggau gcguaacagu uauggcucaa      720

gacaaaccaa caguagauau agaauuagua acuacaacag uaucaaauau ggcugaagua      780

aggaguuauu guuaugaggc aaguaucuca gauauggcaa gugacaguag gugcccaacc      840

caaggcgagg cuuaucuaga uaaacaaagu gauacucaau acguauguaa aaggacgcua      900

guagauagag gauggggaaa cgguugugga cuauuuggaa aaggaagccu aguaacaugu      960

gccaaauuua cauguucaaa gaaaaugaca ggaaaaagua uacaacccga aaaucuagaa     1020

uauagaauua ugcuaagugu acauggaucu caacacucag gaaugauagu aaaugacaua     1080

ggacaugaaa cagaugaaaa uagagcaaaa guggaaguua caccaaauag uccaagagca     1140

gaagcuacac uggguggauu cgggagcuua ggguuagauu gugaaccaag gacaggauua     1200

gauuucucag aucuauacua ucuaacaaug aacaacaaac auugguuagu ucauaaagaa     1260

ugguuucacg acauaccacu accauggcac gcaggagcag auacaggaac accacauugg     1320

aacaauaaag aagcauuagu agaguucaaa gacgcacaug cuaaaagaca gacaguaguu     1380

guucuaggaa gccaagaagg agcuguacau acagcacuug caggagcacu ugaagcagaa     1440

auggauggug caaaagggag acuuuucagc ggacacuuaa aaugccgaau aaaaauggau     1500

aaauuaagau uaaaaggugu cucguauucu uuauguacag cagcauuuac auuuaguaaa     1560

guaccagcag aaacauuaca ugguacggua acagucgaag uacaauaugc aggaagagau     1620

ggaccaugua aaguaccagc acagauggca guagauaugc aaacauuaac accuguaggg     1680

agauuaauaa cggccaaucc uauaauaaca gaaaguacag aaaauucacg caugaugcuu     1740

gaacucgauc caccauucgg agauaguuau auagugauag gaguagguga uaagaaaaua     1800

acccaucauu ggcauagaag uggaaguacu auagguaaag cauucgaagc aacagugaga     1860

ggagcuaaac gaauggcagu auuaggcgau acagcuuggg auuuugguag uguaggugga     1920

guauucaaua gucuaggaaa aggaguacac caaguauucg gagcggcauu uaagagcuug     1980

uucggaggaa ugagcugguu cucacaaaua cuuauaggua cauuauuagu auggcuagga     2040

uuaaauacua aaaacggaag uauuucauua gcaugucuug cucuaggugg ugcaaugauu     2100

uuucuaagca cugccguuuc ugcagacguu gguuguagcg uagauuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  4842
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 1 opt

<400>  4842
gggagaaagc uuaccaugac uagcguagga auaguaggau uauuauuaac gacagcaaug       60

gcugcagaag uaacucgacg cggaucagca uauuauaugu accucgacag aaaugacgca      120

ggagaagcaa uuaguuuccc uacaacauua gggaugaaua aauguuauau ccaaaucaug      180

gauuuaggac acauguguga ugcaacaaug agcuacgaau guccaauguu agacgaaggu      240

guagaaccag acgacguaga cuguuggugc aauacaacau caacaugggu aguauauggu      300

acaugccauc auaagaaagg cgaagcuaga agaaguagac gagcaguaac acuaccauca      360

cauagcacaa gaaaauuaca aacaagaucu caaaccuggc uagaaucaag agaauauacu      420

aaacauuuga uaagaguaga aaauuggaua uuuagaaauc caggauucgc uuuagcagca      480

gcugccauug ccuggcuuuu aggaucauca acaagccaaa aaguaauaua uuuaguaaug      540

auauugcuua uagcgccagc auacagcaua agauguauag gaguuucaaa uagagauuuu      600

gucgaaggaa ugagcggagg aacaugggua gauauaguac uugaacaugg aggaugcgug      660

acaguuaugg cccaagacaa accuaccgua gacauagaau uaguuacaac uacuguaucu      720

aauauggccg aaguaagaag uuauugcuac gaggcuucaa uuagugacau ggcaucagau      780

ucuagaugcc caacucaagg ugaagcauac cuagacaaac aaagugacac gcaauacguc      840

uguaaaagaa cauuaguaga cagaggaugg ggcaauggau gcggauuauu cggaaaaggc      900

agcuuaguaa cuugugcaaa auucgcaugu agcaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaacu uagaauacag aaucauguug ucaguucaug gcagccaaca uucaggaaug     1020

auaguaaacg auacuggaca ugaaaccgau gaaaauagag caaaaguaga aauaacucca     1080

aauaguccua gagccgaagc cacauuaggu gguuuuggau cauuaggacu agauugugaa     1140

ccacgaacag gacuagauuu uagugauuua uauuaucuaa cgaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ucaugauaua ccacuuccau ggcaugcagg agccgauacu     1260

ggaaccccac acuggaauaa uaaagaagca uuaguugagu ucaaagaugc ccacgcaaag     1320

agacaaacag uaguaguauu aggaucacaa gaaggggcag uacauaccgc cuuagcugga     1380

gccuuagaag cagaaaugga cggagcuaaa ggaagacuga guagcggaca cuugaaaugu     1440

cgacugaaaa uggauaaguu aagguuaaaa ggaguaaguu auaguuuaug uacagcugca     1500

uuuacauuua ccaaaauacc agccgaaacc cugcauggga cgguuacagu agaaguacaa     1560

uaugcaggaa cggauggacc auguaaagua ccagcgcaga uggcaguuga uaugcaaaca     1620

cuaacaccag uaggaagacu uauaaccgca aauccgguga uaacagaaag uacagaaaac     1680

uccaaaauga uguuagaauu agauccacca uuuggagacu cauauauagu gauaggagug     1740

ggagaaaaga aaauaacaca ccauuggcau agaaguggaa guacaauagg aaaagcguuc     1800

gaagcaaccg uaagaggagc aaaaagaaug gcaguucuag gagauacugc augggauuuc     1860

gguagcguag gaggagcauu aaauucacua ggaaaaggaa uccaucaaau auuuggagca     1920

gcauucaaaa guuuauucgg aggaaugagu ugguuuuccc aaauacuaau aggaacacua     1980

uuaauguggu uaggauugaa uacgaaaaau ggaucuauau cgcugaugug ucuagcauua     2040

ggaggagucu uaauuuucuu aucaacagca guaucagcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4843
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 1 opt

<400>  4843
gggagaaagc uuaccaugac aucaguugga auagucggac uucuuuugac aacagcaaug       60

gcugcugaag uuacgcgaag aggaagugcc uauuacaugu auuuggacag aaaugaugca      120

ggagaggcua uuucauuccc aacaacauua gggaugaaca aauguuauau acaaauaaug      180

gaucuugguc auacaugcga cgcgacgaug ucauacgaau guccaaugcu ugaugaagga      240

guagaaccug acgauguaga cuguuggugc aacacaacau caacuugggu aguauaugga      300

acuugucauc acaaaaaagg ggaggcaagg agaucacgua gagcaguuac acuaccuuca      360

cauaguacaa gaaaacuaca aacaagauca caaacauggc ucgaaucacg agaauauaca      420

aaacauuuaa uaaggguaga aaauuggauu uucagaaacc caggauuugc ccuugcagca      480

gcagcaauug cauggcuuuu aggaaguagc acaagucaga aagugauaua cuuagugaug      540

auuuuacuua uagcuccagc auauucuaua agaugcauag gaguaaguaa uagagacuuc      600

guagaaggaa ugaguggggg uacuugggua gauguuguac uugaacacgg cggaugcgua      660

acaguaaugg cccaagauaa accaacugua gauauagaau uaguaacgac aacaguuuca      720

aauauggcag aaguaagauc auauugcuac gaagcaucca uaucagacau ggcuucugau      780

ucuagauguc caacucaagg ugaagccuau uuagauaaac aaucagauac ucaauaugua      840

uguaaaagaa ccuuaguaga cagagguugg ggaaauggau gcggauuauu uggaaaagga      900

agucuaguaa cgugugcaaa auuugcaugc aguaaaaaaa ugacuggaaa aaguauucaa      960

ccagaaaacc uugaauauag aauaauguug aguguacaug guagccaaca uucaggaaug     1020

auaguaaaug auacaggcca cgaaacagac gaaaauagag caaaaguuga aauaacacca     1080

aauaguccca gagccgaagc aaccuuagga ggauucggau cucuaggauu agacugcgaa     1140

ccuagaacag gacuagacuu uucagaucuu uacuauuuaa caaugaauaa uaaacauugg     1200

cuagugcaua aggaaugguu ucacgauaua ccauuaccau ggcaugcagg agcugauaca     1260

gguacaccac auuggaauaa uaaagaagca cuuguagaau ucaaagacgc acacgcuaaa     1320

cgacaaacag uaguaguauu agguagucaa gaaggagcag uucauacagc auuagcuggg     1380

gcauuagaag cagaaaugga uggagcaaag ggaaggcuau caucaggaca uuuaaaaugc     1440

agauuaaaaa uggauaaauu gagacucaaa gguguaucau auucucuaug uacagcagcu     1500

uucacuuuua caaaaauacc agcagaaacu cuucauggga cuguuacagu ugaaguacaa     1560

uaugcaggaa cagauggucc augcaaagua ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccag uaggaagacu gauaacagca aacccaguaa uaacagaaag cacagaaaau     1680

ucaaaaauga uguuagaauu agauccucca uucggagauu cauauauagu cauaggagua     1740

ggagaaaaaa aaauaacaca ucauuggcau agaucaggau caacuauagg caaagcauuu     1800

gaagcaacag uaagaggcgc aaaaagaaug gccguacuag gagacacagc uugggacuuc     1860

ggaagcguag gaggagcacu aaauaguuua ggaaaaggaa uucaccaaau auuuggagca     1920

gcauuuaaaa guuuauucgg aggaaugagc ugguucucac aaauauuaau agguacuuua     1980

cuaauguggu uaggacuaaa ugccaaaaac ggaucaauaa gcuuaaugug cuuagcucuu     2040

ggaggugugc uaauauuccu uucaacagca guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4844
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 1 opt

<400>  4844
gggagaaagc uuaccaugac aucaauugga auaauugguc uuuuacuuac uacagcaaug       60

gcagcagaaa uaacaagaag aggaucagca uauuauaugu auuuagaccg uagcgaugcc      120

ggaaaagcaa uaucauucgc uacaacauua ggaguuaaua aaugucaugu ucaaauaaug      180

gacuuaggac auauguguga ugcaacuaug ucuuaugaau guccaaugcu agacgaagga      240

guagaaccag augauguaga uugcuggugu aauacuacua gcacaugggu uguauauggu      300

acaugucauc auaagaaagg cgaagcuaga agaaguagac gagcaguaac uuuaccaucu      360

cauucgacaa gaaaauugca gacgagauca caaacauggu uagaaaguag agaauauacu      420

aaacauuuaa uuaaaguaga aaacuggaua uuuagaaauc cggguuuugc auuaguggca      480

gucgcuauug caugguuauu aggcucauca acgagucaaa aaguaauaua ucuaguaaug      540

auacuauuga uagccccagc cuauucaaua agaugcauag gaguaaguaa uagagauuuu      600

guagaaggua ugagcggagg cacuugggua gaugucguuu uagaacacgg aggaugcgua      660

acaguaaugg cucaagacaa accaaccgua gauauagagu uaguuacaac aacaguaagu      720

aauauggcag aaguucgauc uuacuguuau gaagcaucaa uaucagauau ggcuagcgac      780

ucuagaugcc ccacacaggg agaagccuac uuagauaaac aaagugauac ccaauacgua      840

uguaaaagaa cccuaguaga uagaggaugg ggcaaugggu gcggacuauu ugguaaagga      900

ucucuaguga caugcgcaaa auucacaugc ucaaagaaaa ugacaggaaa aaguauccaa      960

ccagaaaauu uagaauauag aauaauguua ucuguacaug gcucacaaca uucagguaug     1020

auaggauaug aaacugauga agacagagca aaaguagaag ucacaccaaa cuccccaaga     1080

gcagaagcaa cacuuggcgg guuuggaagu uuaggauuag acugugaacc uagaacagga     1140

uuagacuuca gugauuuaua uuaccuaacg augaacaaua aacacugguu aguacauaaa     1200

gaaugguuuc acgacauacc auuaccgugg caugcaggag cagauacugg aacaccucau     1260

uggaacaaua aagaagcauu ggucgaguuc aaagaugcuc augcaaaaag acaaaccgua     1320

guaguauuag gaucacaaga aggagcugua cauacagcau uagcaggagc gcuagaagca     1380

gaaauggaug gagcaaaagg aaggcuauuc ucaggccauc uaaaauguag gcuuaaaaug     1440

gauaaacuac gacugaaagg aguaaguuac ucauuaugua cagcagccuu caccuucacg     1500

aaaguaccag ccgaaacucu acacggaaca guaacuguag aaguacaaua ugcagguaca     1560

gauggacccu guaaaauacc aguacaaaug gcaguagaua ugcaaacauu aacaccagua     1620

ggaagauuaa uuacugcaaa uccaguaaua acagaaagua cagaaaauuc aaaaaugaug     1680

cuugaacuug aucccccauu uggagauuca uauauaguaa uaggaguagg agauaagaaa     1740

auaacacauc auuggcauag aucaggaucg acaauuggaa aagcauucga agcaacagua     1800

agaggagcga aaagaauggc uguauuagga gacacagcgu gggauuuugg aucaguggga     1860

ggugucuuua auagcuuagg aaagggaaua caccaaauau ucggugccgc guuuaaauca     1920

cucuuuggag gcaugucaug guucagucaa auacuaauag gaacucuguu aguguggcua     1980

ggauuaaaua cgaaaaaugg aagcauuagc uuaacauguu uagcauuagg aggagugaug     2040

auauuccuaa guacagcagu aucagcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4845
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 1 opt

<400>  4845
gggagaaagc uuaccaugac aucaguagga auuguuggau uauuacuaac aacagcuaug       60

gcagcugagg ucacaagaag aggaucugca uauuauaugu auuuagauag gaaugaugca      120

ggagaagcua uaaguuuccc gacaacccua gguaugaaua aauguuauau ucaaauaaug      180

gaucuaggac acauguguga ugcuacaaug ucauacgaau gcccuauguu agaugaaggc      240

guagaaccag acgauguaga uugcuggugu aauacaacga gcacaugggu aguauaugga      300

acuugccauc auaagaaagg agaagcuaga agaaguagga gagcuguaac auuaccuagu      360

cauaguacaa gaaaauuaca aacaagaucg caaacauggu uggaaucaag agaauauacg      420

aaacauuuaa uaagaguaga aaauuggaua uuuagaaauc caggguucgc uuuagcagcg      480

gcugcuauug caugguuauu agggaguagc acuucacaaa aaguaauaua uuuagugaug      540

auauuauuaa uagcuccagc auauucaaua agaugcauag gagugucaaa uagagauuuc      600

guagaaggaa ugucgggugg aacaugggua gauguaguuu uggaacaugg cgguugcgua      660

acaguaaugg cucaagacaa accuacagua gauauagaau uaguaacaac aacaguaagu      720

aauauggcag aaguuagauc uuauugcuau gaagcaucaa uuucagauau ggcaagugac      780

ucacgaugcc ccacacaagg ggaagcauac cuagacaaac aaucagauac ucaauacgua      840

uguaaaagaa cauuaguaga ccgggguugg ggaaacggau gcggucuauu cggaaaagga      900

agucuaguaa caugugcuaa auuugcaugu aguaagaaaa ugacagggaa aaguauucag      960

ccugaaaacu uagaauauag aauaaugcug ucuguacaug gaagucaaca uagugguaug     1020

auaguaaaug auacaggaca ugaaacagac gaaaauaggg caaaagugga gaucacgcca     1080

aacaguccua gagcagaagc uacacuagga gguuuuggaa gucuagggcu agauugugaa     1140

cccagaacug gauuagauuu uucggauuua uacuacuuaa caaugaacaa caaacacugg     1200

uugguccaua aagaaugguu ucaugauaua ccauuaccau ggcaugcggg cgcagauaca     1260

ggaacaccac auuggaauaa caaagaagca uuagucgaau uuaaagaugc ccaugcaaaa     1320

agacaaaccg ugguagucuu aggaagucaa gaaggagcag uacauacagc acuagcuggc     1380

gcgcuugaag cagaaaugga uggagcuaaa ggaaggcuau cgucaggaca uuuaaaaugu     1440

agguugaaaa uggauaaacu aaggcuaaaa ggaguuaguu auagcuugug uacugcagca     1500

uucacguuua caaaaauacc agcagaaaca uuacacggua caguuacagu ugaaguacaa     1560

uaugcaggga ccgauggacc auguaaagua cccgcacaaa uggcuguaga uaugcagaca     1620

cuaacacccg uaggaagacu aauaacagca aauccuguca uaacagaaag uacagaaaac     1680

ucaaaaauga ugcuugaauu agauccacca uuuggagauu cuuacauagu aauaggagua     1740

ggagaaaaga aaauaacuca ucacuggcau cgaaguggaa guacaauagg aaaagcauuu     1800

gaagcaaccg uuagaggagc aaaaagaaug gcaguauuag gagacacagc uugggauuuu     1860

ggaagcguag gaggagcauu aaacucauua gggaaaggua uacaucaaau uuucggagca     1920

gcauuuaaaa gucuauuugg gggaauguca ugguuuaguc aaauauuaau agguacauug     1980

cuaauguggu uaggacuaaa uacuaaaaau ggaaguauaa guuuaaugug uuuagcacuu     2040

ggaggaguac uaauauuuuu aagcacagca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4846
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 1 opt

<400>  4846
gggagaaagc uuaccaugac cucaaucggu auuauaggac uacuauuaac aacagcaaug       60

gcggccgaaa uaacgagaag aggaucagca uauuacaugu aucuagaccg aagugaugca      120

ggaaaagcaa uaucuuuugc uacaacacua ggaguaaaca aaugucaugu acaaauuaug      180

gacuuaggac auauguguga cgcgacaaug ucauacgaau gucccauguu agaugaagga      240

guugaaccag augauguaga cuguuggugu aacacaacca guacuugggu aguauacggu      300

acaugccauc acaagaaagg agaagcaaga agaaguagac gagcagugac auuaccaagc      360

cacucaacga gaaaauuaca aacaagaagu caaacauggc uagaaaguag agaauacacu      420

aaacaucuaa uaaaaguaga aaauuggauc uucagaaauc caggauuugc auuaguagca      480

guugcaauug caugguuauu aggaucauca accagccaaa aaguuauuua uuuaguaaug      540

auccuauuaa uugcaccagc auacaguauu agaugcaucg gcguuucaaa uagagauuuu      600

guugaaggua ugucaggagg aacaugggua gauguaguau uagaacaugg uggaugugua      660

acuguuaugg cacaagauaa accuacagug gacauugaau uaguaacaac aacaguaucc      720

aacauggcag aaguuagauc auacugcuau gaagcaagca uaagugacau ggcuucagau      780

ucaagauguc cuacacaagg agaagcauau cuggacaaac aaucagauac ucaauauguc      840

ugcaaaagaa cauuaguuga uagaggcugg ggaaaugggu gcggcuuauu cggaaaaggu      900

agucuaguca caugcgcuaa auuuacaugu aguaagaaaa ugacaggaaa aucaauccag      960

ccagaaaacu uagaauacag aauuauguug ucaguacaug gaucucaaca uagugguaug     1020

auuguaaacg auacaggaua cgaaacagau gaaaauagag ccaaaguaga aguuaccccu     1080

aacucaccaa gagcagaagc aacucuagga ggauuuggau ccuuaggacu agauugugaa     1140

ccaagaacag gcuuagauuu uucagaucua uauuaccuua caaugaacaa uaaacacugg     1200

uuaguacaua aagaaugguu ccacgauaua ccacuaccau ggcacgcagg agcggacacc     1260

gguacaccac acuggaauaa uaaagaagca uuaguugagu ucaaagaugc ucaugccaaa     1320

agacaaacag uaguaguuuu aggaucucaa gaaggagcag uacauacagc uuuagcagga     1380

gcauuagaag cagaaaugga cggagcaaaa ggaaaauuau uuucaggaca uuuaaaaugc     1440

agauuaaaaa uggacaaauu gagauuaaaa ggcguaaguu auucacuaug uacggcagca     1500

uucacauuua cuaaaguccc agccgaaaca cugcauggaa caguuacagu agaaguucag     1560

uacgcaggca cagauggacc augcaaaaua ccuguacaaa uggcaguaga uaugcaaacc     1620

cuaacaccag ucggaagacu uauaacugcc aauccaguua uaacagaauc aacugaaaac     1680

uccaaaauga uguuagaauu agauccacca uuuggagaca guuauauagu aauuggagua     1740

ggagacaaga aaauuacaca ucacuggcau cgaaguggaa guacaauagg aaaagcauuu     1800

gaagcuacag uaagaggagc uaaaagaaug gcaguacuag gugauacagc augggauuuu     1860

gguucuguag gagguguauu uaauagucuu ggaaaaggaa uacaucaaau auuuggagcc     1920

gcauuuaaau cauuauuugg aggaauguca ugguucucac aaauacuaau uggcacauua     1980

uuaguauggc uaggacuaaa caccaaaaac ggaagcauaa guuuaacaug ucuagcacua     2040

ggaggaguaa ugauauuucu aaguacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4847
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 1 opt

<400>  4847
gggagaaagc uuaccaugac aucaauagga auuauugguc uacuucuaac aacagcaaug       60

gcugcagaaa uaacaagaag aggaagugca uauuacaugu auuuagacag aucagacgcu      120

ggaaaagcga ucaguuucgc aacaacuuua ggaguaaaua aaugucaugu acaaauaaug      180

gauuuagguc acauguguga cgcaacuaug uccuaugaau guccaauguu agacgaagga      240

guugaaccag augauguuga uuguuggugu aauacaacau cuacaugggu cguauaugga      300

acaugucaca ggaaaaaagg ggaagcaaga agaucuagaa gggcaguaac cuuaccaagu      360

cauucaacaa gaaagcuaca aacaagaagc caaacauggc uagaaaguag agaauacacu      420

aaacacuuaa uuaaaguaga aaauuggaua uuuagaaauc caggauucgc auuaguugca      480

guagcaauag caugguuauu aggcucguca acaagucaaa aaguuaucua uuuaguaaug      540

aucuuacuaa uagcuccagc cuauucaauu aggugcaucg gcguaucaaa uagagacuuu      600

guagaaggaa ugagcggagg uacaugggua gacguaguau uagaacaugg aggaugcgua      660

acaguuaugg cucaagauaa accaaccgua gauauagaac uaguaacaac aacaguuagu      720

aauauggcag aaguuagaag cuauugcuau gaagcgucua uaucagauau ggcaucagau      780

aguagguguc caacacaggg agaagcauac uuagacaaac aaucggauac ucaauaugua      840

uguaaaagaa cauuaguaga uagaggcugg gguaacggau gcggauuauu cggaaaagga      900

ucauuaguga ccugugcuaa auuuaccugc ucaaaaaaaa ugacgggcaa aucaauacaa      960

ccugaaaauc uagaauaccg cauaaugcuu aguguacaug gaucccagca uucaggaaug     1020

auaguaaacg auacaggcua cgaaacagau gaaaauagag caaagguaga aguuacaccu     1080

aauucaccac gagcagaagc aacauuagga ggauuuggaa gucuagguuu agauugugaa     1140

ccaagaacag gauuagauuu uucagaucua uauuauuuaa caaugaacaa uaaacauugg     1200

cuaguacaua aagaaugguu ucaugauaua ccauuaccuu ggcaugcagg ugcagauaca     1260

ggaacaccuc auuggaauaa uaaagaagcc cuugucgagu ucaaagaugc acacgccaaa     1320

agacaaacug uagucguacu aggaagucaa gaaggugcag uacacacagc uuuagcagga     1380

gcacucgaag cagaaaugga cggagcgaaa ggaaaacugu ucagcggaca uuuaaaaugc     1440

cgauuaaaaa uggacaaauu gagacuaaaa ggaguaucau acucuuuaug uacagcagca     1500

uuuacauuua caaaaguucc agcagaaaca uuacauggaa caguaaccgu agaaguacaa     1560

uaugcaggaa cugacggucc augcaaaaua ccaguucaaa uggcaguaga caugcaaaca     1620

uuaacuccag uggguaggcu aauuacagca aacccuguaa uaacagaaag cacggaaaac     1680

ucuaaaauga uguuagaacu agauccacca uuuggagauu cauacauagu aauuggagua     1740

ggagauaaaa aaauuacaca ucauuggcau agaaguggau caacaauagg aaaagcauuc     1800

gaagcaacug uacgaggggc aaaaagaaug gcaguacuag gcgacacagc augggauuuu     1860

ggaaguguug gggguguauu uaauucuuua ggaaaaggaa uacaucaaau auuuggagcg     1920

gcauucaaau cauuauuugg aggaaugagc ugguuuaguc aaauuuuaau agguacuuug     1980

cuaguauggu uaggauuaaa uacaaaaaac ggaucaauaa gccuaaccug uuuggcuuua     2040

ggaggaguaa ugauauucuu aagcacagca gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4848
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 1 opt

<400>  4848
gggagaaagc uuaccaugac cagcauagga auaaucggac uguuauuaac gacagcuaug       60

gcagcugaaa uaacaagaag gggauccgcg uacuacaugu acuuagauag aucugaugcu      120

ggaaaagcaa uaucauucgc uacaacacuc ggaguaaaca aaugucaugu acaaauaaug      180

gaucuaggac acauguguga ugccacaaug uccuacgaau guccuauguu agaugaagga      240

guugagccag augauguaga uugcuggugu aauacaacuu ccacaugggu aguauauggc      300

acaugccauc acaagaaagg agaagcuaga agaaguagga gagccguaac acuaccaagc      360

cauaguacaa ggaaauuaca aacaagaagu cagacuuggc uagaaaguag agaauauaca      420

aaacaucuga uaaaaguaga aaauuggaua uucagaaauc caggauuugc ucuaguagca      480

guagcaauag caugguuauu aggaagcuca acaagccaaa aaguuaucua uuuaguaaug      540

auauuauuaa uagcuccagc guauuccaua agaugcauag gaguaaguaa uagagacuuu      600

guagaaggaa ugaguggugg gacaugggua gauguugucu uagaacaugg gggaugugua      660

acagugaugg cucaagacaa accaacaguu gacauagagc uaguaacuac aacaguaagu      720

aacauggcag aaguaagauc auacuguuau gaagccucca uaucagauau ggcauccgac      780

agucgauguc caacacaagg ugaagcauau uuggacaaac aaagugauac acaguaugua      840

ugcaagagaa cauuaguuga uagaggaugg gguaacgggu guggauuauu uggaaaaggu      900

ucacugguga caugugcaaa auuuacaugc ucaaagaaaa ugacuggaaa aagcauacaa      960

ccagaaaauu uagaauauag aauuauguua ucaguucacg gaagccaaca uaguggaaug     1020

acuguaaaug acauaggaua ugaaacagac gaaaacagag caaaaguuga aguaacgcca     1080

aauucaccua gagcagaagc uacauuagga ggauuuggca guuugggauu agauugugaa     1140

cccagaacag gacuugacuu cagugauuua uauuaucuga caaugaacaa uaaacauugg     1200

uuaguacaua aagagugguu ucaugauaua ccauuaccgu ggcaugcagg agcagacaca     1260

ggaacuccac acuggaacaa uaaagaagcu cuaguagaau uuaaagacgc acacgcuaaa     1320

agacaaacag uaguagucuu agggucacaa gaaggcgcag uacacacagc uuuagcagga     1380

gcauuagaag cagaaaugga cggcgccaaa ggcaaacuau uuucuggcca uuuaaagugu     1440

aggcuaaaaa uggacaagcu aagauuaaaa gggguaucau auucauuaug cacagccgcc     1500

uuuacauuua caaagguucc agcagaaaca cugcauggaa cagugacagu agaaguccaa     1560

uaugcaggaa cagauggacc auguaaaaua ccaguacaaa uggccguaga caugcaaaca     1620

uuaacaccag uaggaagacu cauaacagca aacccuguca uuacagaaag cacagaaaau     1680

ucuaaaauga ugcuagaauu agauccaccu uuuggagauu cuuauaucgu gauaggaguu     1740

ggagauaaga aaauaacaca ucauuggcau agauccggaa guacaauagg aaaagcguuu     1800

gaagccacag uaaggggagc aaaaaggaug gcaguacucg gagauacagc gugggauuuu     1860

gggucaguug gaggaguuuu uaauagucua ggaaaaggua uacaccaaau auuuggggca     1920

gcauucaaau cuuuauucgg aggaaugagc ugguuuucac aaauauuaau aggcacacua     1980

cuuguauggu uaggauuaaa uaccaaaaau ggaucaauaa guuugacaug ccuagcgcua     2040

ggagggguaa ugauauuucu gucaacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4849
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 1 opt

<400>  4849
gggagaaagc uuaccaugac aaguauaggu auaguaggcc uucuacuaac gacagcaaug       60

gcagcugaaa uaacaagaag agguucagca uauuauaugu aucuagauag aagugacgca      120

ggaaaagcua uuagcuucgc aacuacuuua ggaguuaaua aaugucaugu acaaauaaug      180

gacuuaggac auaugugcga ugcaacaaug aguuaugaau gcccaauguu agaugaagga      240

guagaaccag acgauguaga uugcuggugc aauacuacau cgacaugggu aguguacggc      300

acaugucauc acaagaaagg agaagcgaga agaucaagaa gggcaguaac acuaccaagc      360

cauaguacga gaaaauuaca aacacgaucu caaacauggu uagaaucaag agaauauaca      420

aaacacuuaa uaaaagucga gaacuggaua uuucggaauc caggauucac ccucguugca      480

guagccauag cuuggcuacu aggauccuca acuagccaaa aaguaauuua uuuaguaaug      540

auauuauuaa uagcuccagc auauucaaua agauguauag guguaucuaa cagagauuuu      600

gucgaaggca ugucaggagg aacaugggua gauguaguuc uagaacaugg aggaugcgua      660

acaguuaugg cacaagacaa acccacugua gauauagaau uaguaacgac aacaguauca      720

aauauggcag aaguacgaag cuauugcuau gaagccucaa uaucagacau ggcuucagau      780

ucaagauguc caacacaggg ugaagcauau cucgacaaac aaucggacac acaauaugua      840

uguaaacgca cauuaguaga uagaggaugg gguaauggau gcggucuauu cggaaaagga      900

ucauuaguaa caugugcaaa auucacuugc ucuaagaaaa ugacgggaaa aucaauucag      960

ccagagaauu uagaauaccg uauaauguua ucuguucaug gaucacaaca uucugggaug     1020

auaguaaaug augaaaauag agcaaaagua gaagugacac caaauucacc aagagcagaa     1080

gcuacacuag gaggauuugg aucacuagga uuggauugcg aaccacgaac uggcuuagau     1140

uuuucagauc uauacuauuu aacaaugaau aacaaacauu ggcuaguaca uaaagaaugg     1200

uuccacgaua uaccauuacc auggcaugcu ggggcagaua cuggaacacc acauuggaau     1260

aauaaggaag cauuaguaga auuuaaagau gcucacgcaa aaagacaaac aguagucgug     1320

uuaggaaguc aagaaggagc aguacauacc gcauuagcug gugcacuaga agcagaaaug     1380

gauggagcaa aaggaagauu auuuucugga caucuaaaau gccgauuaaa gauggauaaa     1440

cuuagauuaa aagguguuag cuacagucua uguacagcag cauucacauu caccaaagua     1500

ccugcagaga cccuacacgg aacaguuacc guagaaguac aauaugcagg gacugaugga     1560

ccaugcaaag uaccugcaca aauggcagua gacaugcaaa cauuaacccc aguaggaaga     1620

uuaauaacag cuaauccagu aauaacagaa ucaacagaaa acagcaaaau gaugcuagaa     1680

uuggacccac cuuuuggaga uaguuacaua guaauaggag uaggagauaa gaaaauaaca     1740

caucacuggc auaggagugg uaguacaauu ggaaaagcau uugaagcaac aguuagagga     1800

gcuaaaagaa uggcagucuu aggagauaca gcaugggauu uuggaagugu aggagguguu     1860

uuuaauucgu uaggaaaagg aauacaucaa auauuuggag ccgcuuucaa aagucuuuuu     1920

ggaggaaugu caugguuuuc acaaauucuu auagggacau uauuaguuug gcuuggauua     1980

aacacuaaaa acggaaguau aucuuuaaca ugccuagcac uaggaggggu aaugauuuuu     2040

cuaaguacag caguaagugc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4850
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 1 opt

<400>  4850
gggagaaagc uuaccaugac gucaauuggu auaauaggcc uauuacuaac aacagcuaug       60

gcggcagaaa uuacaagaag gggaucagca uauuauaugu accuagauag aucagaugca      120

gguaaagcua uaaguuuugc uacuacauug ggaguaaaua aaugucacgu acaaauaaug      180

gaucuggguc auaugugcga cgcuacaaug ucguaugaau guccaaugcu agacgaaggg      240

gucgaaccug augauguaga uuguuggugu aauacuacau cgacaugggu aguauauggc      300

accugucauc acaaaaaagg agaagcaaga agaucaagaa gggcaguaac acuaccaagu      360

cauagcacua gaaaauuaca aacaagaagc caaacauggu uagaaagccg agaauauaca      420

aaacauuuaa uaaaaguaga gaauuggaua uuuagaaauc caggauuugc cuuaguagca      480

guugcaauag cauggcuacu uggaucaucc acuagucaaa aaguaauaua ucuaguaaug      540

auauuauuaa uagcaccagc auauaguauu agauguauag gaguuucaaa uagagacuuu      600

guugaaggua ugucuggagg aacaugggua gacguagucu uagaacaugg aggaugugua      660

acaguaaugg cacaagauaa accuacagua gauauagaac uaguaacaac aaccguuagu      720

aauauggcag aaguaagguc auauuguuac gaagcaucaa uaucugacau ggcaagugau      780

agucgaugcc caacacaagg ugaagcguau uuggauaaac aaucagauac acaauaugua      840

uguaaaagaa cucuaguaga uagagguugg ggcaacggcu guggauuauu uggaaaggga      900

agccuaguaa caugugcaaa auucacuugc aguaaaaaaa ugacaggaaa aucaauacaa      960

cccgagaacc uagaauauag aauaauguua ucaguacaug ggucgcagca cucaggaaug     1020

auaggauaug aaacagauga aaauagggcu aaaguugagg uuacgccaaa uucuccacgc     1080

gcagaagcaa cauuaggugg auucggcuca cuaggacuag auugugagcc aagaacagga     1140

uuagacuuua gugaucuaua uuaucuaaca augaauaaua aacauugguu aguacauaaa     1200

gaaugguuuc augacauacc auuaccaugg cacgcaggag cugauacagg cacuccgcau     1260

uggaauaaua aagaagcacu cguagaguuu aaagacgcac acgccaaacg acaaacagug     1320

guuguacuag gaucacaaga aggagcaguu cacacagcac uagcaggagc acuagaagcc     1380

gaaauggaug gagcuaaagg uaaauuauuu aguggacauc ugaaaugcag acuaaaaaug     1440

gacaaacuua gauugaaagg uguaaguuau aguuuaugca ccgcagccuu uacuuuuaca     1500

aaaguaccag cagaaacacu ucauggcaca guaacaguug aaguacaaua ugcugguaca     1560

gauggaccuu guaaaauucc uguacaaaug gcagucgaca ugcaaacauu aacaccaguu     1620

ggacgccuaa uaacggcuaa uccaguaaua acugaaagca cagagaauag uaaaaugaug     1680

uuagaauuag aucccccauu cggagauagu uacauuguaa ucggaguugg agauaaaaaa     1740

auaacgcauc auuggcacag aagcgguuca acaauaggaa aagcauuuga agcaacaguc     1800

agaggugcga aaagaauggc aguccucggc gauacagcau gggauuucgg uucaguggga     1860

ggaguauuca auucucuagg gaaagguaua caucaaauau uuggugcagc cuuuaaauca     1920

uuauuuggag gaaugaguug guuuucccaa auauuaauug gaacauuauu agugugguua     1980

gggcucaaca caaaaaacgg aaguauauca cuaacuuguu uagcacuagg aggaguaaug     2040

auauuuuugu caacugcugu aagcgcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4851
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 1 opt

<400>  4851
gggagaaagc uuaccaugac aagcguagga auaguagguc uauuacuaac gacagcaaug       60

gcagcagaag uaacuagaag aggaucggca uauuauaugu auuuagacag aaaugaugcg      120

ggggaagcaa uaucuuuucc gacaacauua ggaaugaaua aauguuauau acaaaucaug      180

gaccuuggac auauguguga cgcgacaaug ucauaugaau guccaauguu ggaugaagga      240

guagaaccag augauguuga uuguuggugu aacacgacau cuacaugggu aguauauggc      300

acuugccauc auaaaaaagg agaagcgaga agaagcagaa gagcagugac acuaccuagu      360

cauucaacaa gaaaauuaca aacaagauca caaacauggu uagaaucaag agaauacaca      420

aaacacuuaa uucgaguuga aaacuggaua uucagaaacc caggauuugc cuuagcugcu      480

gcagcaauag caugguuauu aggcaguagu acaagccaaa aaguaauaua ucuaguaaug      540

auccuauuaa uagccccagc auauaguaua agauguauug guguaucuaa ucgagauuuu      600

guagaaggaa ugucaggagg aacaugggua gacguaguau uagaacacgg aggauguguc      660

acaguaaugg cacaagacaa accaacagua gauauugaau uagucacaac uacaguaagc      720

aauauggcag aaguaagaag uuauuguuau gaagccucca uaucagauau ggcaagugac      780

ucaagauguc cgacucaagg agaagcuuau cuugauaaac aaagcgauac ucaguacgua      840

ugcaaaagaa cacuuguuga uagaggcugg ggaaacggau gcggauuauu cggaaaagga      900

agccugguaa caugcgcuaa auuugcaugu aguaaaaaaa ugacaggaaa aagcauacaa      960

ccagaaaauc uagaauaucg aauaauguua ucaguacacg gcucacagca uucaggaaug     1020

auuguuaacg acacaggaca cgaaacugac gagaauagag caaaaguuga aauaacacca     1080

aacucaccga gagcagaagc aacauuagga ggcuucggca gccuaggacu agacugugaa     1140

ccacgaacag gcuuagacuu uagugaucua uauuaccuca cuaugaauaa caaacauugg     1200

uuaguucaca aagaaugguu ccaugauaua ccuuuaccau ggcaugcagg ugccgauacu     1260

gguacaccac acuggaacaa caaagaagca uuaguagaau uuaaagaugc acaugcaaaa     1320

agacaaacag uuguuguauu aggaucacaa gaaggagcag uacacacagc guuagcaggg     1380

gcacuagaag cagaaaugga cggggccaaa ggacggcuaa guucaggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu aagacuaaaa ggaguaucgu acucgcugug cacagcagcc     1500

uuuacauuca caaaaauucc ugccgaaaca cuacacggaa cuguaacagu agagguacaa     1560

uacgcaggua cugauggacc auguaaagua ccagcgcaaa uggcaguaga uaugcaaaca     1620

uugacaccag uaggaagauu aauaacagca aauccaguaa uaacagaauc uacagaaaac     1680

ucaaaaauga ugcuugaauu agauccaccu uuuggagaua guuacauagu aauaggggua     1740

ggagaaaaaa agauaaccca ccauuggcau agaucaggau caaccauagg gaaagcauuu     1800

gaagccacug ucagaggagc uaaaaggaug gcaguuuuag gagacacagc augggauuuc     1860

ggaagcgugg guggcgcacu caauagucuu ggacgaggaa uacaucaaau auuuggugcc     1920

gcauucaaaa gucuuuuugg aggaaugagu ugguuuucuc agauacuaau uggaacauua     1980

uuaauguggc uaggauuaaa uacaaaaaau ggaaguauuu cauugaugug uuuagcacua     2040

ggaggaguau uaauauuucu aaguacggca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4852
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 1 opt

<400>  4852
gggagaaagc uuaccaugac aagugucgga auagugggau uacuuuuaac aacagcaaug       60

gcugcugaag uaacuagaag agguucagca uacuacaugu acuuagacag aaaugaugca      120

ggagaagcaa uaaguuuucc gacaacauug ggaaugaaua aauguuauau acaaauaaug      180

gauuuaggac auauguguga ugcaacaaug ucauaugaau gucccauguu agacgaaggc      240

guagaaccag augaugugga uugcuggugc aauacaacau caacuugggu agucuacgga      300

acaugucauc acaagaaagg ugaagcaaga agaagcagac gagcaguaac auuaccuagu      360

cauagcacua gaaaguuaca aacacggagu caaacuuggu uagagaguag ggaauauaca      420

aagcauuuaa uaagaguaga aaacuggaua uucagaaauc caggucuagc ucuagcugca      480

gcugcaauug cauggcuauu aggaucaagu accucacaaa aagugauaua uuuaguaaug      540

auuuuacuaa ucgcaccagc auauucaaua agaugcauag gaguuagcaa ucgagauuuc      600

guagaaggaa ugaguggagg aacaugggua gauguuguau uagaacacgg uggauguguu      660

acagugaugg cgcaagauaa accuacagua gauauagaau uaguuacaac aacuguuagc      720

aauauggcgg aaguaagaag uuauuguuac gaagccagua uaagugauau ggcaucggac      780

aguagaugcc ccacacaagg ugaagcauau cuagauaaac aaucagauac acaauauguu      840

ugcaaaagaa cauuaguaga cagagguugg ggaaaugguu gcggucuuuu uggaaaaggu      900

agucuaguaa caugcgcaaa auucgccugu agcaagaaaa ugacagguaa aaguauacag      960

ccugaaaacu uagaguauag aauaaugcua ucaguacacg ggagccaaca cagcggaaug     1020

auuguuaacg acacgggaca ugaaacagau gaaaacagag caaaaguaga aauuacaccg     1080

aauagcccuc gagcagaagc cacacuaggu gguuuuggaa gccuaggauu ggacugugaa     1140

ccaaggacag gucuagauuu uagugaucua uauuacuuaa caaugaacaa uaaacauugg     1200

uuaguacaua aagaaugguu ucaugacauc ccuuuaccau ggcaugcagg agcagauacc     1260

ggaacuccac auuggaacaa uaaagaagca uuaguagaau uuaaagaugc acacgcaaag     1320

agacaaacag uaguaguauu aggaagccaa gaaggagccg uacauacagc cuuagcaggc     1380

gcguuagaag cagaaaugga uggagcaaag gguagauuaa guucgggaca uuugaaaugu     1440

aggcucaaaa uggauaaauu acgauugaaa ggaguaaguu acucuuugug uacagcagcu     1500

uuuacuuuua caaaaauacc agcagaaaca uuacauggua cagucacugu ggaaguacaa     1560

uaugcaggaa cagauggacc augcaaagua ccugcacaaa uggcaguaga caugcaaaca     1620

cuuacaccgg uagguagauu aauaacagcu aauccaguaa uaaccgaaag uacagaaaac     1680

agcaaaauga uguuagaauu agacccgcca uuuggagauu cauauauagu aauuggagua     1740

ggagaaaaga aaauaacaca ccauuggcau agaucaggaa guacuauagg uaaagcguuc     1800

gaagcuacag uaagaggagc aaaaagaaug gccguguugg gcgacacugc augggacuuu     1860

ggaucgguug gaggugcacu aaauucauua gguaaaggca uucaccaaau auuuggagca     1920

gcauuuaagu cauuauuugg aggaaugagc ugguuuucac aaauacuaau uggaacguua     1980

uuaauguggc uaggauuaaa uacuaaaaau gguagcauua guuuaaugug ucuagccuug     2040

ggaggagugu uaaucuuccu uaguacagca guuagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4853
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 1 opt

<400>  4853
gggagaaagc uuaccaugac aucaguaggu aucguaggau uacuauuaac aacagcaaug       60

gcagcagaag uuacgagaag agguucagcc uacuauaugu aucuugauag gaacgaugca      120

ggggaagcua ucaguuuucc uacaacauua ggaaugaaca aaugcuauau ucaaauaaug      180

gauuuaggac auauguguga cgcuacuaug agcuaugaau guccuaugcu agacgaaggc      240

guagaaccug augacguaga cuguuggugu aauacaacau cgaccugggu aguguaugga      300

acgugucauc auaagaaagg agaagcaaga agaagcagac gagcuguaac auuaccauca      360

cauaguacaa gaaaauuaca aacacgauca caaacauggc uagaaaguag agaauauaca      420

aaacauuuaa uuagaguuga aaauuggaua uuuaggaauc cagguuuugc auuggcagca      480

gcugcaauug cguggcugcu cggcaguuca acaucacaaa aaguaauaua ucuaguaaug      540

auauugcuaa uagcuccagc uuauucaaua agaugcauag gaguuucaaa uagagacuuu      600

guugaaggca ugaguggagg cacaugggua gacguaguau uagaacacgg aggaugcgug      660

acuguaaugg cacaagauaa accaacagua gauauugagu ugguaacaac aacaguguca      720

aauauggcag aaguaagaag uuauuguuac gaagcuagca uaagugauau ggcaucagac      780

agcagauguc cuacucaagg cgaagcauau cucgacaaac aaagugauac acaauacgua      840

uguaaaagga cacuggucga cagagggugg ggaaacggcu gugguuuguu uggaaaagga      900

agucuaguaa ccugugcaaa auuugcuugu aguaaaaaga ugacgggaaa gaguauccaa      960

ccagaaaacu uggaauauag aauaauguua uccgugcacg ggagucaaca uucaggaaug     1020

auaguaaacg auacaggaca ugaaacagac gaaaauagag caaaagugga aauuacucca     1080

aauaguccaa gggccgaagc uacucuuggc ggauuuggau cauuaggauu agacugcgaa     1140

ccgaggacag gacuagauuu cagugauuug uacuaucuaa caaugaacaa caaacauugg     1200

uuaguccaua aagaaugguu ccaugauauu ccauuaccgu ggcaugcugg ugcggauacu     1260

ggaacaccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc ucaugcaaaa     1320

agacaaaccg uaguaguuuu agguagucaa gaaggagcag uacacacagc auuggcggga     1380

gcccuagaag cagaaaugga uggagcaaaa gggagacuau cuucaggaca cuugaaaugu     1440

agauugaaaa uggauaaacu cagauuaaaa gggguaucau auucauuaug uacagcugca     1500

uucacauuua caaaaauacc agcugaaaca cuacauggua cuguuacggu agaaguacaa     1560

uaugcaggca cagacggacc uuguaaaguu ccagcacaaa uggcaguaga caugcaaaca     1620

uuaacaccug uaggaagauu aauaacagca aauccaguga uaacagaauc aacagaaaau     1680

aguaaaauga uguuagaauu agauccacca uuuggcgauu cauauauagu aauaggugua     1740

ggagaaaaga aaaucaccca ccauugguau agaagcggaa gcacaauagg uaaagcauuu     1800

gaagcaacag uaagaggagc aaaaaggaug gccguucuag gagacacugc augggauuuu     1860

ggaaguguag gaggagcacu aaacucuuua ggaaaaggua uucaucaaau auuuggagca     1920

gcauuuaaau cguuauucgg aggaaugagc ugguuuucac aaaucuuaau aggaacauua     1980

cuaauguggc uuggauugaa uaccaaaaau ggaucgauau cuuuaaugug ucuagcacua     2040

gggggaguuu uaauauuucu aucaacagca gugagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4854
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 1 opt

<400>  4854
gggagaaagc uuaccaugac gucuguugga auaguuggau uauuacuaac aacagcaaug       60

gcagcagaag uuacaagaag aggcucagcc uauuacaugu acuuagauag aaaugacgca      120

ggagaagcua uuagcuuucc gacuacucua ggaaugaaua aaugcuacau acaaauaaug      180

gacuuaggac acaugugcga ugcaacaaug aguuacgaau guccaaugcu agacgagggu      240

guugaaccgg augauguaga uuguuggugu aacaccacaa gcacuugggu gguauaugga      300

acgugucacc acaaaaaagg agaagcacgu agaucacgaa gggcaguaac acucccauca      360

cauaguacua gaaaauuaca aacaagaagu caaacguggu uagaaucaag agaauacacc      420

aaacacuuaa uuagaguaga aaauuggaua uuucgaaauc cagguuuugc ucuagcagca      480

gcagcaauag caugguuauu aggaagcucg acaagucaaa aaguaauaua uuuaguaaug      540

auacuauuaa uagcuccggc uuauucaaua agauguauag gaguaucaaa uagagacuuu      600

guagaaggaa ugucuggagg aacuuggguu gaugugguau uagaacaugg gggaugugua      660

acaguaaugg cccaagauaa accaacaguu gauauagaac uuguuacaac aaccguaagu      720

aacauggccg aaguaagauc cuauugcuau gaagcaucaa uaucggauau ggcaagugau      780

ucaagauguc cuacucaagg agaagccuac uuagacaaac aaagcgauac acaauaugua      840

ugcaaaagga cacuaguaga ucgugguugg ggaaacggau guggauuguu cggaaaaggu      900

agccucguaa caugugcaaa auuugcuugu aguaaaaaaa ugacaggaaa aucaauacaa      960

cccgaaaauu uagaauauag aauaaugcua aguguccacg ggagccaaca uagcgguaug     1020

auaguuaacg auacaggaca ugaaacugau gaaaauaggg caaaagucga aauaacacca     1080

aauaguccaa gagcagaagc aacauuaggu ggauuuggau cuuuaggacu agacugugaa     1140

ccaaggacug guuuagauuu uagcgacuua uauuaucuaa caaugaacaa uaaacauugg     1200

uuaguacaua aagaaugguu ccaugauaua ccauuaccau ggcacgcagg agcagauacc     1260

gguaccccgc acuggaauaa uaaagaagca cuaguugaau uuaaagacgc acaugcaaaa     1320

cgucagacag uaguaguauu aggaagucaa gaaggcgcag uacauacagc auuagcagga     1380

gcacuugaag cggaaaugga uggugcaaaa ggaagauuaa guucaggaca uuugaagugu     1440

cgguuaaaaa uggacaaacu aagauuaaaa ggaguaucau auucgcuaug uacagcugcu     1500

uuuacauuca cuaaaauccc agcugaaaca cuacauggaa caguaacagu agaaguucaa     1560

uaugcaggua cugacggacc augcaaaguc ccagcacaaa uggcagugga uacacagacc     1620

uuaacacccg ugggaagacu uauuacugca aauccaguaa uaacugaauc aacugaaaau     1680

ucaaaaauga uguuagaauu agauccacca uuuggagacu cguauaucgu aauuggugua     1740

ggagaaaaaa aaauaacuca ucacuggcau agaucaggau caacaauagg aaaagcauuc     1800

gaagcuacag uaagaggcgc aaaaagaaug gcaguccuag gagauacugc augggacuuc     1860

ggaagcguag gaggcgcacu uaauucucua gguaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaaa gcuuauuugg aggaaugucc ugguuuucac aaauacuaau aggaacuuug     1980

uuaauguggc uuggucuuaa uacaaaaaau gggucaauaa guuuaaugug ccuagcuuua     2040

ggaggggucu uaauauuucu uuccacugca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4855
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 1 opt

<400>  4855
gggagaaagc uuaccaugac aucaguuggc auuguaggau uacuauuaac aacagcuaug       60

gcagcagaag uaacacgaag gggaucagca uauuauaugu aucuagauag aaacgaugca      120

ggagaagcaa ucagcuuccc aacaacacua gggaugaaua aauguuauau acaaauuaug      180

gauuugggcc acauguguga cgcaacaaug aguuaugaau gcccaaugcu ugacgaagga      240

guugaacccg augauguaga cugcuggugu aauacaaccu caacuugggu aguuuaugga      300

accugucauc acaagaaagg agaagcucga agaucaagac gagcaguaac auuaccuagu      360

cauucaacga gaaaauuaca aacuagauca caaacuuggc uugaauccag agaauacaca      420

aaacaucuaa uaagaguuga aaacuggauc uuuagaaacc cugguuuugc cuuagcagcg      480

gcugcaauag caugguuacu aggaucauca acaagucaga aagucauaua uuuagucaug      540

auauuacuaa uagcuccugc auacucuaua agauguauag gugugaguaa uagagacuuu      600

guggaaggaa ugucuggugg aacaugggug gacguaguuu uagagcacgg gggaugcguu      660

acaguaaugg cccaagauaa accaacaguu gauauugagc uaguaacaac gaccguaagc      720

aauauggcag aaauaagauc auauugcuau gaagccucaa uuagugacau ggcuagcgau      780

agcagaugcc caacacaggg agaagcauac cuggacaaac aaucagacac acaauaugua      840

ugcaaaagaa cauuaguaga cagaggaugg ggaaaugguu guggacuauu uggaaaaggg      900

ucauuaguaa caugcgcaaa auuugcaugu ucaaagaaaa ugaccgguaa aucaauacaa      960

ccagaaaauu uagaauacag aauaaugcua ucaguacacg guagucaaca uagcgggaug     1020

auuguaaacg auacaggaca cgaaacagau gaaaaucgcg caaaaguaga aauaacgcca     1080

aauaguccua gagcugaagc aacauugggu ggauuuggga guuuaggacu agauugcgaa     1140

ccuagaacag gacuagauuu uagugacuua uauuaucuca caaugaauaa uaaacacugg     1200

uuaguucaca aagaaugguu ucaugauaua ccauuaccau ggcaugcagg agcagauaca     1260

gguacacccc auuggaacaa uaaagaagcu cuaguagagu uuaaagacgc acaugcaaaa     1320

cgacaaacag uaguaguuuu agguagucaa gaaggcgcag ugcauacagc acuagcagga     1380

gcauuagaag cagaaaugga cggugcgaaa ggucgauuaa guaguggaca uuuaaaaugc     1440

cgacuaaaaa uggacaaauu acgauuaaaa gguguaucau acucauuaug uaccgcugca     1500

uucacuuuua caaaaauacc ugcagaaaca uugcacggaa cuguaacagu agaaguacaa     1560

uaugcgggua cagauggacc augcaaaguu ccagcccaaa uggcugugga uaugcaaaca     1620

cuaacaccag uagguagacu uauaacggca aauccaguaa uaacagaaag uacagaaaau     1680

ucaaaaauga uguuagaauu agauccccca uuuggagaca gcuauauagu uauaggagua     1740

ggagagaaaa agauaacuca ucauuggcau cgaagcgguu caacaauugg aaaagcauuu     1800

gaagcaacug uaagaggagc aaaaagaaug gccgucuuag gggacacugc uugggauuuc     1860

ggaucaguag gcggugcauu aaauucacua ggaaaaggua uucaccaaau auuuggagca     1920

gcauucaaau cauuauucgg aggcaugagu ugguucaguc aaauacuaau aggaacauua     1980

cuaauguggc uuggacuaaa cacuaaaaau gguucaauaa guuuaaugug ucuugcauua     2040

ggcggcguac uuauauuuuu aucaacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4856
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 1 opt

<400>  4856
gggagaaagc uuaccaugac cucaguaggu auaguuggau uacuacuaac aaccgcaaug       60

gcugcagaag uaacaagaag aggaucagca uauuauaugu auuuagacag aaacgaugca      120

ggggaagcga uaucuuuucc aacaacuuua gggaugaaua aauguuacau acaaauaaug      180

gaccuagguc acauguguga ugcaacuaug ucauaugaau gcccaaugcu cgaugaagga      240

guagaaccag acgacguaga cuguuggugu aauacaacuu caacaugggu aguauauggc      300

acaugccauc auaagaaagg agaagcaaga agaucacgua gagcaguaac auugccauca      360

cauucuacca gaaaacuaca aacaagaagc caaacauggu uagaaucaag agaauacaca      420

aaacacuuaa uaagaguaga aaauuggauu uuuagaaacc cagguuucgc uuuagcugca      480

gcagcuauag cuuggcuauu agggaguagc acaucacaaa aagucauaua ucuaguuaug      540

auccuccuga uagcaccagc auauaguaua cgauguauag gaguaucaaa cagggacuuc      600

guugaaggaa ugaguggagg aacuugggua gauguuguuc uagagcaugg aggcugugua      660

acuguaaugg cacaagacaa accaacagua gacauagagc uaguaacaac uacuguaagc      720

aauauggcug aaguaagauc uuauugcuau gaagcaagca uaucagauau ggcaagugac      780

agucgauguc caacccaagg agaggcuuau uuagauaaac aauccgauac ucaauauguu      840

uguaaacgaa cccuagugga cagaggaugg gguaacggau guggacuauu ugggaaagga      900

agccucguga caugugcaaa auuugcuugu ucgaaaaaga ugacaggaaa aucaauacaa      960

ccugaaaauc uagaauauag aauaaugcua ucuguacacg gaagccaaca uaguggaaug     1020

auaguaaaug acacagguca ugaaacagac gagaacagag caaaaguuga aauuacgccc     1080

aauagcccuc gagcagaagc uacacuaggg ggauuugguu cucuaggacu agacugugaa     1140

ccaagaacag gccuagauuu uucagaccua uauuauuuaa caaugaauaa uaaacacugg     1200

cuaguccaua aagaaugguu ccacgauaua ccauuacccu ggcacgccgg agcagacaca     1260

ggaacaccuc acuggaauaa uaaagaagcc cuuguagaau uuaaagaugc acacgcaaaa     1320

cgucaaaccg uaguaguauu aggcagucaa gaaggagcag uucauacagc auuagcugga     1380

gcauuagaag cagaaaugga cggagcaaag ggcagacuau ccucaggaca ucuaaaaugc     1440

agauugaaaa uggauaaacu acgauuaaaa ggaguaaguu auucacugug uacagcagca     1500

uuuacguuua caaaaauacc agcagaaaca uuacacggaa cagucacagu agaaguucaa     1560

uaugcaggaa cagacggucc auguaaagua uuagcacaaa uggcuguaga uaugcaaaca     1620

cuaacaccug uaggaagauu aauuacugca aauccuguua ucacugaaag cacagaaaac     1680

ucaaaaauga ugcuugaauu agauccacca uuuggcgauu cauacaucgu cauuggagua     1740

ggagaaaaga aaauaacgca ucacuggcac cgaaguggau caacaauagg uaaagcauuu     1800

gaagcaacag uacguggagc uaaaagaaug gcaguuuuag gagauacagc uugggauuuu     1860

ggaagugucg guggagcacu aaacucacua ggaaaaggaa uacaucaaau auuuggagca     1920

gcauucaaau cauuauuugg aggcauguca ugguuuaguc aaauuuuaau aggaacucua     1980

cuaauguggu uaggauuaaa cacuaaaaac gguucaauau ccuuaaugug ucuagccuua     2040

ggaggaguuc uuauauuucu cagcacagcu guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4857
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 1 opt

<400>  4857
gggagaaagc uuaccaugac gaguguagga auaguaggau uguugcuaac aacagcuaug       60

gcugcagaag uaacuagaag aggcagugcc uacuacaugu acuuagacag aaaugacgcu      120

ggugaagcaa uuagcuuucc aacaacacua ggaaugaaca aauguuauau acaaauaaug      180

gacuuaggcc auauguguga ugcaaccaug ucauacgagu guccaacauu agacgagggc      240

guugaaccag acgacguaga uugcuggugu aauaccacuu caacaugggu aguauauggu      300

acuugucacc auaagaaagg ugaagcaaga agaucacgua gagcagugac cuuaccuuca      360

cauucuacaa gaaaauuaca aacaaggagu caaacauggc uugaaucaag agaguauaca      420

aaacaccuaa uaaggguuga aaauuggaua uuucgaaacc cgggguuugc acuagccgca      480

gcugcaauag cauggcuccu aggaaguuca acuagccaaa aaguaauaua ccuaguaaug      540

aucuugcuua uagccccagc auacaguaua agauguauug gagugaguaa uagagauuuc      600

guagaaggaa ugucaggagg gacuuggguu gauguaguau uagaacaugg aggaugcgua      660

accguaaugg cacaagauaa accgacgguu gauauagaau uaguaaccac aacaguaagu      720

aauauggcug agguaagaag uuacuguuau gaggcaucaa uaucagacau ggccagcgac      780

agucguuguc caacgcaagg agaagccuau uuagacaaac aaucagauac acaauaugua      840

uguaaaagaa cucuaguuga uagaggaugg ggaaacggcu gcggacuuuu uggaaaagga      900

ucauuaguga caugcgcaaa auuugccugu aguaagaaaa ugacagguaa aucuauacaa      960

ccugaaaauu uagaauauag aauuauguua ucaguacacg gaucacaaca uucggggaug     1020

auaguaaaug auaccggaca cgaaacagau gaaaauagag cuaaaguuga aaucacacca     1080

aacaguccaa gagcagaagc aacauuaggc ggauucggau cacuaggccu agauugugaa     1140

ccaagaacag gacuagauuu uagcgaucuu uauuacuuaa caaugaauaa uaaacauugg     1200

uuaguucaua aagaaugguu ucaugauaua ccccuuccau ggcaugccgg agcagauacu     1260

ggaacaccac acuggaauaa caaagaagca uuagucgaau uuaaggaugc acaugcaaaa     1320

agacaaacug uuguaguauu aggaucacaa gaaggagcag uacauacagc uuuagcugga     1380

gcacuagaag cugaaaugga cggagcaaaa ggacgauuaa gcucuggaca ccuaaaaugu     1440

agacuaaaaa uggauaaacu aagacucaaa ggaguuucuu auucguuaug uacugcagca     1500

uuuacuuuua caaaaauucc agcagaaacg cuacauggaa caguaacugu agaaguacaa     1560

uaugccggaa cugauggacc auguaaaguc ccagcacaaa uggcaguuga uaugcaaaca     1620

cuaacuccag uaggaagauu aauaaccgcu aauccaguca uaacagaauc aacagaaaau     1680

ucaaaaauga uguuagaauu ggauccuccc uuuggugaca guuacauagu aauaggagua     1740

ggagaaaaga aaauaacaca ucacuggcau agaaguggau caacuauagg uaaagcauuu     1800

gaagcuacag uacgaggagc aaaaagaaug gccguucuug gagacacagc uugggacuuu     1860

ggaaguguag gaggagcucu aaauagcuua ggaaagggua uacaucaaau auuuggagcc     1920

gcuuuuaaaa gucuauucgg aggaauguca ugguuuucac aaauauuaau aggaacacua     1980

cuaauguggu uaggacuaaa uacgaaaaau ggcagcauua gcuuaaugug uuuagcacua     2040

ggaggaguuc uaauauucuu aucaacagcc guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4858
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 1 opt

<400>  4858
gggagaaagc uuaccaugac aucaguagga auagugggau uacuuuuaac aacggcaaug       60

gcagcugaag uaacaagaag aggcagugcu uauuauaugu auuuagacag gaaugaugca      120

ggugaagcaa uuaguuuucc uacaacuuua ggcaugaaca aauguuacau acaaauaaug      180

gaucuaggac auaugugcga ugcaacaaug ucauaugagu gcccuauguu agaugaaggu      240

guugaaccag acgaugugga uugcuggugu aauaccacca gcacuugggu aguauacgga      300

acaugucauc auaaaaaagg cgaagcaaga agaaguagga gagcuguaac ucucccuagu      360

cauucuacga gaaaauuaca aacaagauca caaacuuggc uugaaucaag agaauauaca      420

aaacauuuaa uacgaguaga aaacuggaua uuuagaaauc cagguuucgc acuggcagca      480

gcagcaauag caugguuacu aggaucauca acaagccaaa aaguaauaua cuuaguaaug      540

auucuguuaa uagcaccagc guauagcauc cguuguauag guguaucaaa uagagauuuu      600

gucgaaggaa ugaguggagg aacauggguu gauguaguuc uagaacacgg aggcugcguc      660

acagucaugg cacaagauaa accaacggua gauauugaau uaguaacaac aaccgucagc      720

aacauggcag aaguaagauc auacuguuau gaagcaucaa uauccgauau ggcaucagac      780

ucuagauguc caacucaggg agaggcauau uuagauaaac aaucagauac gcaauaugua      840

uguaaaagaa cauuaguaga uagagggugg ggcaauggau guggauuauu uggaaaggga      900

ucuuuaguua cuugugcaaa auuugcaugc ucaaaaaaaa ugacaggaaa aaguauucaa      960

ccagaaaauu uagaauaccg aauaaugcuu aguguacacg gaagucaaca uucaggaaug     1020

aucgucaaug acacagggca cgaaacagac gaaaacagag caaaaguaga aauaacaccu     1080

aauucuccaa gagcagaagc aacuuuaggu ggauucggaa gcuugggccu ugauugugaa     1140

ccaagaaccg ggcuugauuu uagcgauuua uacuauuuaa caaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ccaugauaua ccucuaccau ggcacgcagg ugcagauaca     1260

ggaacaccac acuggaacaa uaaagaagcc uuaguugaau uuaaagaugc acaugcgaaa     1320

agacaaacag ucgucguacu aggaagucaa gaaggagcag uacauacugc ucuugcaggg     1380

gccuuagaag cagagaugga uggagcaaaa ggaagauuau cuucgggaca ucucaaaugu     1440

agauuaaaaa uggauaaauu aagacuaaaa ggugucuccu acagucuaug uacggcagca     1500

uucacauuua caaaaauacc ugcagaaacc uuacauggaa caguaacagu cgaaguacaa     1560

uaugcaggaa cagauggucc auguaaagua ccagcacaaa uggcaguuga uaugcagaca     1620

uuaacacccg uaggaagauu aauaacagca aauccaguca uaacugaauc aacagaaaac     1680

aguaaaauga uguuagaauu agauccgcca uucggagaua guuauauagu uauuggaguc     1740

ggugaaaaaa aaauaacuca ccauuggcac agaucaggau caacaauagg aaaagcauuu     1800

gaagcaacag uaagaggagc aaaaagaaug gcaguguuag gagauacugc augggauuuu     1860

ggaaguguag gaggagccuu gaauucauua gguaaaggua uacaccaaau auuuggggcc     1920

gcauuuaaaa gcuuauuugg aggaaugagu ugguuuucac aaauucuaau aggaacauua     1980

uuaauguggc uuggauuaaa uacuaaaaac gguucuauuu cacuaaugug uuuagcauua     2040

ggcgggguuc uaauauuucu uucuacgguc guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4859
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 1 opt

<400>  4859
gggagaaagc uuaccaugac aucaguuggu auaguuggac uacuauuaac aacagcgaug       60

gcagcugaag uaacaagaag aggaucagca uauuacaugu acuuagauag gaacgaugcu      120

ggugaagcaa uuucauuucc uacaacacua ggaaugaaua aauguuauau acaaauaaug      180

gaccuagguc auaugugcga ugcuacuaug ucuuaugaau gcccaaugcu agaugaagga      240

guagaaccag augauguaga cuguuggugu aacacaacca gugcaugggu aguauauggu      300

accugccauc acaagaaagg agaagcuaga agaucuagac gagcuguaac auuaccuagc      360

cauucaacga gaaaauuaca gacaagacca caaacuuggc uagaaucuag agaauacacc      420

aaacaucuaa uaaggguaga aaauuggaua uuuagaaauc cagguuuugc acuagcugcc      480

gcagcaauug cuugguuacu agguucauca acaucucaaa aaguaaucua ucuaguuaug      540

aucuuacuaa uagcuccugc auauaguaua agaugcauag gaguaagcaa cagagauuuu      600

guagaaggua ugucaggugg aacauggguu gauguaguac uugaacacgg uggaugugug      660

acuguaaugg cacaggauaa acccacagug gacauagaau uaguaacaac aacuguguca      720

aauauggcug aaguaagauc auacugcuau gaagcaucaa uuagugauau ggcauccgau      780

aguagauguc cuacacaagg agaagcauau cuagauaaac aaagugacac acaauauguc      840

uguaaaagaa cguuaguuga uagaggaugg ggaaauggau gcggauuauu uggaaaagga      900

ucacuuguaa ccugugcaaa auuugcaugu ucaaagaaaa ugacaggcaa aaguauacaa      960

ccagaaaauc uagaauauag aauaauguua ucgguccaug gaucacaaca uagcgguaug     1020

auuguaaaug auacaggaca cgaaacagac gaaaauaggg caaaaguuga aaucacccca     1080

aacucaccca gagcagaagc aacucuggga ggauuuggca gccugggauu agauugugaa     1140

ccaagaacug gacuugauuu uagugaucua uacuaucuua caaugaacaa uaaacauugg     1200

cucguacaua aagaaugguu ccaugauaua ccauuaccuu ggcaugcagg agccgauaca     1260

ggaacaccac acuggaacaa uaaagaagca uuaguagagu ucaaagacgc acaugcaaaa     1320

aggcaaacag uaguuguacu aggaagccaa gaaggagcag uucauacugc auuagcgggu     1380

gcauuagaag ccgaaaugga uggagcgaaa gguagauuau cuucuggaca cuugaaaugu     1440

cgcuuaaaaa uggauaaacu aagacuaaaa ggaguaaguu acucauuaug cacagcagca     1500

uucacauuua caaaaauucc ugcggaaaca cuacacggaa cuguaacagu agaaguacaa     1560

uaugcuggaa cugauggacc auguaaaguu cccgcacaaa uggcaguuga caugcaaaca     1620

uuaacaccgg uaggaagauu gauuacugcg aacccuguua uaacagaaag uacggaaaau     1680

aguaaaauga ugcuagaauu agauccaccu uuuggagaua guuauauagu aauaggagua     1740

ggugaaaaga aaauaacaca ucauuggcac agaagcggau caacaauagg aaaagcauuu     1800

gaagcuacag uuagaggagc uaaacgaaug gccguacuug gagacacagc cugggauuuu     1860

ggcuccguag gaggagcauu aaacagucua gguaaaggca uacaucaaau auuuggagca     1920

gccuuuaaaa guuuauuugg agguauguca ugguuuucac aaauacuaau aggaacauua     1980

uuaauguggc uaggacuaaa cacuaaaaau ggaucgauaa gccuuaugug cuuagcacua     2040

ggagguguuu uaauauuucu aagcacagca gugucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4860
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 1 opt

<400>  4860
gggagaaagc uuaccaugac aucuguggga auaguagguu uauuauuaac uaccgcaaug       60

gcugccgaag uaacaagaag aggaucagca uauuauaugu accuugauag aaaugaugca      120

ggagaagcaa uaucuuuucc aacaacauua gguaugaaua aaugcuacau acaaauuaug      180

gauuuaggac auauguguga ugcaacgaug ucauaugaau guccaaugcu ugaugaagga      240

guugaaccug acgauguaga uuguuggugu aauacaacaa gcaccugggu uguauaugga      300

acaugucauc auaaaaaagg agaagcucga agaaguagaa gagcaguaac ccuaccgagc      360

cacagcacua gaaaauuaca aacuagguca caaaccuggc uagaaaguag agaauacaca      420

aaacaucuua uaagaguaga aaauuggauc uuuagaaauc cagguuucgc acuagcagca      480

gcagcaauag cauggcuacu agguucgucc acaucaaaaa aaguaauaua uuuaguaaug      540

auauuacuaa uagcaccagc auauucaaua agauguauag guguaaguaa uagagauuuc      600

guugaaggaa ugaguggagg aacaugggua gauguagucu uagaacacgg aggaugugug      660

acuguuaugg cacaagacaa accaacagua gacauagaac uuguaacgac aacaguaagc      720

aauauggcag aaguacguag uuauugcuau gaagcaucaa uuucagauau ggcaucagau      780

uccagauguc caacacaagg agaagcuuau uuagauaaac agucagauac gcaauauguu      840

ugcaaacgaa cccuaguuga cagagggugg ggcaacggau gcggauuguu cggaaaagga      900

ucguuaguaa cuugugccaa auucgcaugu ucuaaaaaaa ugaccggaaa aucaauacaa      960

ccagaaaauu uagaauauag aaucaugcua agcgugcaug gcucacaaca cucuggcaug     1020

auuguaaaug auacaggaca ugaaacugac gagaauagag cuaaaguaga aauaacaccg     1080

aauucaccca gagcagaagc aacauuagga ggauucggau cacugggauu agauugcgaa     1140

ccacgaacag gauuagauuu cagugaccuu uauuauuuaa caaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ccacgacaua ccauuaccuu ggcaugcagg cgccgauaca     1260

ggaacaccac auuggaauaa uaaagaagcu cuaguagaau uuaaagaugc acaugcaaaa     1320

aggcaaacag uagucguucu aggaucacaa gaaggagcag uacauacagc auuagcagga     1380

gccuuagaag ccgaaaugga uggcgcaaaa ggaagacuaa gcagcggaca uuugaaaugu     1440

cgguuaaaga uggacaaguu aagguuaaaa ggcguuagcu acaguuuaug uacagcagca     1500

uuuaccuuca caaaaauccc ugccgaaaca uuacauggaa caguuacagu agaaguucaa     1560

uaugccggga cugacggacc auguaaagua ccagcucaaa uggcaguaga caugcaaaca     1620

uuaacaccug uuggaagacu aauaacagca aauccaguga uaacugagag uacagaaaac     1680

aguaaaauga uguuagaauu ggacccucca uucggugaca gcuauaucgu aauaggagua     1740

ggugaaaaaa aaauaacaca ucacuggcac agaucaggau caacaauagg uaaagcauuc     1800

gaagcaacag uacguggagc aaaacgaaug gcgguucuag gagauacugc gugggauuuc     1860

ggaagcgucg gaggggcauu aaacucauua gggaaaggaa uucaucaaau auuuggugcc     1920

gcauuuaaaa gccuauuugg agguaugagu ugguuuaguc aaauauuaau uggaacacuc     1980

cuuauguggc ucggauuaaa uacuaaaaac ggaucuauua guuuaaugug uuuggcauua     2040

ggugguguac uuauauuuuu aaguacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4861
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 1 opt

<400>  4861
gggagaaagc uuaccaugac gaguguaggu auaguaggau uauuacuaac cacagcuaug       60

gccgcagaag uaaccagaag aggaagugca uacuauaugu acuuagaucg gaaugaugca      120

ggggaagcca uaagcuuucc aacaacucua gguaugaaua aaugcuauau acaaauaaug      180

gacuuaggac auauguguga ugcgacaaug agcuaugaau guccaaugcu agacgaaggc      240

guagaaccag augauguuga cugcuggugu aacacaacaa guacaugggu aguauacggg      300

acaugucacc auaaaaaagg cgaagcgcga agaucaagaa gagcaguuac auuaccaagu      360

cauuccacua gaaaauuaca aacccguuca caaacauggc uugaaucacg agaauacaca      420

aaacaucuua uucguguaga aaacuggaua uuccgaaauc cgggauuugc acuagcagca      480

gcagcaaucg caugguuauu aggaaguucu acaagacaaa aaguuauaua ccucguaaug      540

auacuuuuaa uagcaccagc cuauagcaua agauguauag gaguauccaa cagagauuuc      600

guugaaggaa ugaguggagg aacaugggua gacguagucu uagaacaugg aggaugugua      660

acaguaaugg cucaagauaa accuacaguu gauauagaac ucguaacaac uacaguaagu      720

aauauggccg aaguccguuc auauuguuau gaagcaucaa uaucagauau ggcgagcgac      780

aguagauguc cuacccaagg agaagcauau uuagacaaac aaagugauac acaauauguc      840

ugcaaaagaa cauuagugga cagagguugg gguaauggau gcggacuauu cggaaaagga      900

aguuuaguaa caugugccaa auuugccugu ucaaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaacu uagaauauag aauuauguua ucaguccaug gcucacaaca cagugggaug     1020

auagucaacg acacaggaca ugaaacagau gaaaauagag cuaaaguaga aauuacacca     1080

aauucacccc gagcggaagc aacacuagga ggauuuggau cacuaggauu agauugugaa     1140

ccuagaacag guuuagacuu uagugauuua uacuaucuua caaugaacaa uaaacacugg     1200

uuaguacaca aagagugguu ccaugacaua ccacuuccau ggcaugcagg agcagacacg     1260

gggacgccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc acaugcgaaa     1320

agacaaacgg uaguaguacu agguagccaa gaaggagcag uacacacagc cuuagcaggu     1380

gcauuagaag cagaaaugga cggagcuaaa ggacgguuau caagugggca cuuaaaaugc     1440

agacuaaaaa uggacaaauu aagauuaaaa ggaguaagcu acucauuaug cacagcagca     1500

uuuacauuca cuaaaauccc cgcagagacu uuacauggca cagugaccgu agaaguacaa     1560

uacgcaggca cagauggacc auguaaaguu ccugcacaaa uggcuguaga uaugcaaacg     1620

uuaacaccag uugguagauu aauaacugca aauccuguaa uuaccgaguc cacggaaaau     1680

ucaaaaauga ugcuagaauu agacccacca uuuggagauu cuuauauagu aauaggaguc     1740

ggagagaaga aaauaacuca ucauuggcau agaagcggaa guacaauagg aaaagcauuu     1800

gaagcaacag ucagaggagc caaaagaaug gcaguucuag gagacaccgc uugggauuuu     1860

ggaucaguag gaggugccuu aaauucucua ggaaaaggaa uacaucagau auuuggugca     1920

gcauuuaaaa guuuauucgg uggaaugucg ugguuuucac agaucuuaau aggaacgcug     1980

cuaauguggc uaggacuaaa uacaaaaaau ggaucaauau cauuaaugug ucuagcauua     2040

gguggcguac uaauauuuuu aucaacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4862
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 1 opt

<400>  4862
gggagaaagc uuaccaugac aagugucggc auaguagguc ucuuacugac aacagcgaug       60

gcagcagaag uaacaagaag aggcagugca uacuacaugu auuuagauag gaaugaugcc      120

ggggaagcaa uaucauuccc aacaacuuua gguaugaaua aauguuauau acaaauaaug      180

gauuuggggc auauguguga cgcuaccaug aguuacgaau guccaaugcu ugaugaaggu      240

gucgaaccag augauguaga uuguuggugu aauacaacua guacaugggu aguuuaugga      300

acuugucauc acaaaaaagg agaagcgaga agaagcagaa gagcugugac acuccccucc      360

cauaguacua gaaaacuaca aacacgaagc caaacauggu uagaaucaag agaauauaca      420

aaacauuuaa uaagaguaga aaauuggauu uuuagaaauc caggauuugc auuagcugca      480

gcugcaauag cauggcuccu ggguucauca acuagucaga aagugauuua uuuaguuaug      540

auacuacuaa ucgcacccgc guauucaaua agguguauag guguauccaa cagagauuuc      600

guagaaggaa ugucuggagg aacauggguu gauguaguuu uagaacacgg uggguguguu      660

acaguaaugg cucaagacaa accaacugua gauauugaac uaguaacaac uacuguaagc      720

aauauggcag aagucagaag uuauuguuac gaagccucaa uaagcgacau ggcuucagau      780

ucuagauguc caacacaagg ggaagcauau uuagauaaac aaucagauac acaauauguc      840

ugcaaaagaa cacuagucga cagaggaugg gggaacgguu gcggguuauu cggcaaagga      900

ucauuaguua ccugcgcaaa auucgcaugu agcaaaaaaa ugacggguaa aaguauacaa      960

ccagaaaauc uugaauauag aauaaugcua ucuguucacg guucacaaca uaguggaaug     1020

auagucaaug auacaggaca ugaaaccgau gaaaacagag caaaaguuga aaucacacca     1080

aauucaccuc gagcagaggc uacauuagga ggcuuuggua gucuuggauu agacugugaa     1140

ccgagaacag gauuagauuu uagugauuua uauuauuuaa caaugaauaa uaaacauugg     1200

cugguacaca aagaaugguu ucaugauauu ccacuuccau ggcacgcagg ugcagauaca     1260

gguacaccgc acuggaauaa caaagaagca uuagucgaau uuaaagacgc acaugcaaaa     1320

agacaaacag uaguuguuuu aggcacucaa gaaggugcag uccauacggc acuagcgggg     1380

gcacuagaag cagaaaugga uggugcuaaa ggaagacuaa guucaggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu aagacuaaaa ggaguaucau auucauuaug uacagcagca     1500

uuuacauuua cgaaaauucc agccgaaacc cuacacggua cuguuacugu agaaguccaa     1560

uaugcaggua cagauggacc auguaaagua ccugcgcaaa uggcaguaga uaugcaaaca     1620

cuuacaccag uaggaagauu aauaacugcg aauccaguaa uaacggaauc cacagaaaau     1680

ucaaagauga uguuagaauu agauccaccu uuuggagauu cauacauagu aaucggaguu     1740

ggugaaaaaa aaauaacaca ucauuggcau agaucaggcu caacaauagg aaaagccuuu     1800

gaggcaacag uaagaggcgc caaaagaaug gcaguccuag gugacacagc augggauuuu     1860

ggaucuguag gaggagcauu aaauucccua ggaaaaggaa uacaccaaau uuuuggagcc     1920

gcuuuuaaau ccuuauuugg aggaauguca ugguuuucac aaauucuaau uggaacacua     1980

uugauguggu uaggcuuaaa cacaaaaaac ggaagcauca guuuaaugug ucuggcacuc     2040

ggaggaguac uaauauuccu aucaacagca guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4863
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 1 opt

<400>  4863
gggagaaagc uuaccaugac uucuguagga auaguaggcc uauuguuaac aacugccaug       60

gccgcagaag ucacaagaag gggcagcgca uacuauaugu auuuagauag aaacgaugca      120

ggagaggcaa uuucauuucc aacaacacua ggaaugaaua aauguuauau acaaauaaug      180

gaucuugguc auauguguga cgcaacaaug ucguaugaau guccuaugcu ugacgaaggu      240

guugaaccag augauguaga uuguuggugc aauacaacau caacuugggu aguauacgga      300

accugucauc auaaaaaagg agaggcaaga agaucaagaa gagcaguuac gcuaccuagu      360

cauucgacaa ggaaauuaca aacaagaagu caaacauggc uagaaagcag agaauacacu      420

aaacaccuaa uaagaguaga aaacuggaua uuuagaaauc cuggauuugc acuagcagcu      480

gcggcaauag cauggcuauu gggcucauca acaagccaaa aaguaauaua ucuagucaug      540

auacucuuaa uagcaccagc auacagcuua agauguauag gagucagcaa ucgagauuuu      600

guagagggua ugaguggagg uacaugggug gauguaguuc uugaacaugg agggugugua      660

acaguaaugg cucaagauaa accaacagua gauauagagu ugguaacgac aacagucagc      720

aauauggcag aaguaagaag cuacuguuac gaagcaucaa uaucagauau ggcaagugac      780

ucaagauguc caacacaagg agaagcauau uuagauaaac aauccgauac ucaauaugua      840

uguaaacgaa cguuaguaga cagagguugg ggaaauggau guggccuuuu uggaaaaggu      900

ucacuaguaa cuugugccaa auuugcaugu ucaaaaaaaa ugacaggaaa aucaauacaa      960

ccagaaaauu uagaauauag aaucaugcuu ucaguccaug gaagucagca cucaggaaug     1020

aucgucaaug auacaggaca ugaaacugau gaaaauagag cuaaaguuga aauuacaccg     1080

aauuccccaa gggcugaagc aacucuuggc ggauuuggua gucugggucu agacugcgaa     1140

ccaagaacag guuuagacuu uucagauuua uacuaccuaa caaugaauaa uaaacauugg     1200

uuagugcaua aagaaugguu ucacgauauu ccauuaccau ggcacgcagg ugcagauaca     1260

gguacaccac auuggaauaa uaaagaggca cuaguagaau uuaaagaugc acacgcaaaa     1320

agacaaacag uaguaguauu agguucacaa gaaggagcag uacauacagc uuuagcagga     1380

gcauuagaag ccgaaaugga cggcgccaaa ggaagacuuu caagcgggca uuuaaaaugc     1440

agauuaaaaa uggauaaacu uaggcuaaaa ggaguaaguu auaguuuaug uacagccgcu     1500

uucacauuua cuaaaauacc agcagaaaca cuacacggua caguuacugu ugaaguucaa     1560

uacgcaggua cagauggucc cuguaaagua ccagcacaaa uggcuguaga caugcaaacg     1620

cuuaccccag uaggaagauu aauuacugca aacccaguaa uaacagaauc aacagagaac     1680

agcaaaauga uguuagaauu agauccaccu uucggugauu cauauauagu aauaggagua     1740

ggagaaaaaa aaauaacaca ucauuggcau agaaguggga guacaauugg uaaagcguuc     1800

gaagcuacug uuagaggagc aaaacgaaug gcgguauuag gagauacagc cugggauuuc     1860

ggaaguguug guggagcauu aaauaguuua ggcaaaggaa uacaucaaau auucggagcu     1920

gcauuuaaga gucuauucgg ugguaugagu ugguucucac aaauacuaau uggaacacua     1980

uuaauguggu uaggauuaaa uacaaaaaac ggaaguauau cacuaaugug uuuagcauua     2040

gguggaguuu uaauuuuucu aagcacagca guaucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4864
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 1 opt

<400>  4864
gggagaaagc uuaccaugac gaguguuggc auuguagggu uauuauuaac aacggcuaug       60

gcagcagaag uaacaagaag aggaucagca uauuauaugu auuuagauag aaacgacgca      120

ggagaagcaa uaucuuuccc aacaacacua gguaugaaua aauguuauau acaaauaaug      180

gaucuagguc auauguguga ugcuacaaug agcuaugaau gcccaaugcu agaugaagga      240

guagaaccgg augacguaga cuguuggugu aauacaacau cuacaugggu aguauacgga      300

acaugucacc auaagaaagg agaagcaaga agaucgcgua gagcagucac acuccccuca      360

cauucaacca gaaaauuaca aacuagaagu caaacuuggu uagaaucaag agaauauacu      420

aaacacuuaa uaagaauaga aaacuggaua uucagaaauc caggauuugc auuagcugcg      480

gcagcuauag cauggcucuu aggaucuucu accagucaaa aaguaauaua ccuaguaaug      540

auacucuuaa uagcaccagc auauucuaua cgaugcauug gagugaguaa cagagauuuu      600

guugaaggaa ugucaggagg aacaugggua gacguuguuc ucgaacacgg aggaugugua      660

accgugaugg cucaggauaa accaacagua gauauugaau uaguaacaac aacaguuuca      720

aauauggcag aaguccgaag uuacuguuac gaagcaucca uaucagacau ggcaucggau      780

ucaagauguc caacccaagg agaagccuau uuagauaaac agucagacac ucaauauguu      840

uguaaacgaa ccuuaguaga ucgaggcugg gguaauggau guggacuuuu cgguaaaggc      900

agucuuguaa ccugcgcaaa auucgcaugu aguaagaaaa ugacaggaaa aaguauccaa      960

ccagaaaacc uggaauauag aauuaugcua ucaguccacg gaucacaaca uucaggaaug     1020

auaguaaaug auacugguca ugaaacagau gaaaauaggg caaaaguaga aauaacucca     1080

aauucaccac gagccgaggc cacccuugga ggauuuggau cauuaggccu agauugcgaa     1140

ccuagaacag gauuagauuu uagugauuua uacuauuuaa ccaugaacaa caaacauugg     1200

uuaguucaua aagaaugguu ucaugauaua ccauuaccau ggcaugcugg agcagauaca     1260

gguacaccac auuggaacaa uaaagaagca uuaguugaau uuaaagaugc acacgcuaaa     1320

cgucaaacug uuguuguuuu aggaucacag gaaggagcug uacauacagc auuagcgggu     1380

gccuuagaag cagaaaugga cggagcaaaa ggucgacuaa guagugguca cuuaaaaugu     1440

agauuaaaaa uggacaaauu aagauuaaaa gguguuuccu auuccuuaug uacagcagcg     1500

uuuaccuuca ccaaaauacc agcagaaacc cuacacggaa caguaacagu agaaguacaa     1560

uaugcaggua cagauggacc cuguaaaguu ccugcacaaa uggcaguaga caugcaaacu     1620

uuaaccccag uaggaagauu aauaacagcc aauccaguga uuacagaauc cacagaaaac     1680

ucgaaaauga uguuagaauu ggauccacca uucggugaua guuacauagu uauaggagua     1740

ggagaaaaga aaauuacaca ccauuggcac agaagcggaa gcacaauagg aaaagcauuc     1800

gaagcaacag uaagaggagc aaaaagaaug gcugucuuag gagauacagc augggauuuc     1860

gguucuguug gcggagcuuu gaacaguuug ggcaaaggaa uacaucaaau auuuggggca     1920

gcauuuaaga gcuuauuugg agguauguca ugguucucac aaauauuaau aggaacauua     1980

cuaauguggc uaggauuaaa uacgaaaaau ggcagcauuu cacuaaugug ucuagcauua     2040

gguggagucu uaauauuucu aucaacagca guaucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4865
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 1 opt

<400>  4865
gggagaaagc uuaccaugac aucaguggga auugugggau uauuauuaac aaccgcuaug       60

gcagcagaag ucacuagaag aggaucugca uauuacaugu aucuagauag aaacgaugca      120

ggagaagcaa uaaguuuccc aacaacauua gguaugaaua aauguuacau acagauaaug      180

gaucuagguc auaugugcga ugcaacuaug aguuaugaau gcccaauguu agaugaagga      240

guagaaccag augauguaga uuguuggugu aacacaacau caacaugggu agucuacgga      300

acaugucauc auaagaaagg ugaagcuaga agaagccgca gggcaguaac uuuaccgucg      360

cacucaacaa gaaaacuuca gacaagaagu caaacuuggu uagaaaguag agaauauaca      420

aaacauuuaa uccguguaga aaauuggaua uuaaggaacc caggauucgc auuagcagca      480

gcggcaauag ccugguuacu agguucaagc acgucacaaa aaguaauaua cuuagucaug      540

auacuguuaa uugcaccagc auauagcauu cguuguauug gcguaucuaa cagagauuuc      600

guugaaggaa ugucgggagg aacauggguu gauguaguuc uagaacaugg cgguugugua      660

acaguaaugg cccaagauaa accaacagua gauaucgaac ucguaacuac aacgguaucc      720

aauauggcag aaguucgaag uuauuguuac gaagcaagua uaagugacau ggccuccgau      780

ucaagauguc cuacgcaggg ugaggcuuau cuagacaaac aaucggacac acaauaugua      840

uguaaaagaa cauuaguaga uagaggaugg ggaaauggau guggacuauu uggaaaagga      900

aguuuaguaa ccugugcaaa auuugcuugc aguaagaaaa ugacagguaa aagcauacaa      960

ccagaaaauc uugaauauag aauaaugcug agcguacacg gcagccaaca cucuggaaug     1020

auuguaaacg auacugggca ugaaacagau gaaaaucgag caaaaguaga aauaacacca     1080

aacagcccaa gggcugaagc aacccugggu ggauuuggaa guuugggucu agauugcgaa     1140

ccaagaaccg gucuagauuu cucagauuua uauuauuuaa cgaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ccaugauaua ccauuaccau ggcaugcagg agcugauaca     1260

ggaacaccac auuggaauaa caaagaagca uuaguagagu ucaaagaugc acacgcaaaa     1320

agacaaacug uaguaguacu aggaagucaa gaaggagcag uccacacagc auuagcagga     1380

gcucuagaag cagaaaugga uggugcaaaa ggccgacuau cuucuggaca uuuaaaaugu     1440

agauuaaaaa uggacaaacu aagauuaaaa ggagugucau acucauuaug cacagcagca     1500

uuuacauuua caaaaauucc agcagaaaca cuacauggaa caguaacugu agaaguccaa     1560

uaugccggaa cagacggacc uuguaaagua ccagcacaaa uggccguaga uaugcaaaca     1620

uuaacaccug uagguagacu aauuacagcc aauccaguaa uaacugaauc cacugaaaau     1680

agcaaaauga ugcuagaacu ggauccucca uuuggagauu cauauauagu cauagggaua     1740

ggagagaaaa agauaacaca ccacuggcau agaucuggaa guacuauagg uaaagcauuc     1800

gaagcaacag uaagaggugc aaaaaggaug gcaguauuag gagacacagc cugggauuuc     1860

ggaucaguag gaggugcauu aaauucauua ggaaaaggua uccaccaaau uuucggagca     1920

gcuuuuaaau cuuuauucgg uggaaugagu ugguuuucac aaauauuaau uggaacuuua     1980

cuaauguggc uaggauuaaa uaccaaaaau ggaucaauaa gucuuaugug ucuagcauua     2040

gguggaguac uuauauuucu aaguacagca guaucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4866
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 1 opt

<400>  4866
gggagaaagc uuaccaugac uucaguagga aucgucggac uuuuguuaac aacggccaug       60

gcugcagagg uaacaagaag aggaucagcu uauuacaugu auuuagauag gaaugaugcu      120

ggagaagcca uaaguuuccc aacuacacua ggaaugaaua aauguuauau ucaaauaaug      180

gauuuagguc auauguguga cgccacuaug aguuacgagu guccaauguu ggaugaaggc      240

guagaaccag acgauguuga cuguuggugc aacaccacau caacaugggu aguauauggu      300

acaugucauc auaagaaagg agaagcaaga agaucaagaa gggcaguaac acucccuuca      360

cauucaacaa gaaaauuaca aacgagaagc cagacauggc uagagucaag agaauauacu      420

aaacacuuaa uaagaguaga aaauuggaua uucagaaauc caggguuugc auuagcagca      480

gcagcaauag cuugguuacu aggaaguagu acgucacaaa aaguaauaua ucuuguaaug      540

auuuuauuaa uugcaccagc uuauagcauu agauguauag gcguuuccaa ccgugauuuu      600

guugaaggaa ugucuggagg aacauggguu gauguagugc uagaacacgg aggaugugua      660

acaguuaugg cacaagauaa accaacagug gacauagagc uaguaucaac uacugucagc      720

aauauggcgg aaguaagauc auacuguuac gaagccacaa uaagugauau agcaucagac      780

ucaagaugcc caacacaagg agaagcauau cuagacaaac aaucagacac ucaauaugua      840

uguaaaagaa cauugguaga uagaggaugg ggaaauggau gcggauuauu uggaaaaggc      900

aguuuaguua caugugcaaa auuugccugc aguaagaaaa ugacuggaaa aucaauacaa      960

ccugaaaauu uagaauauag aauaauguua ucaguacaug ggucucagca cucaggaaug     1020

auagucaaug acacaggaca cgaaacugau gaaaauagag cuaaaguuga aauaacacca     1080

aauagcccac gagcagaagc aacauuagga ggcuuuggaa guuuaggacu agauugugaa     1140

ccaagaacag gccuagacuu uucagaucua uauuauuuaa caaugaacaa caaacauugg     1200

cuaguacaca aagaaugguu ccacgauaua ccauugccau ggcacgcagg agcagacaca     1260

ggaacuccac auuggaauaa uaaagaagcu cuuguagaau uuaaagaugc acaugcaaaa     1320

agacaaacag ccguaguacu agguucacaa gaaggagcug uacacacagc acuagcugga     1380

gcauuagagg cagaaaugga uggagcuaaa ggaagauugu cgagugggca ucuaaaaugc     1440

agauuaaaaa uggacaaauu acgauuaaaa ggaguaucuu auucacuaug caccgccgca     1500

uuuacauuua caaaaauacc ggcagaaacc uuacauggaa ccguaacagu ugaaguacaa     1560

uaugcaggaa cagauggucc auguaaagua ccagcucaaa uggcaguuga uaugcaaaca     1620

uuaacaccag ucggaagacu uauaacagca aauccaguaa uaacagaauc cacagaaaau     1680

agcaaaauga ugcuggaacu agauccucca uuuggggacu cauacaucgu aauaggagua     1740

ggagaaaaga aaauaacgca ccauuggcau agaaguggua guacaauagg aaaagcauuc     1800

gaagcuacag uaagaggagc gaaaagaaug gcaguauuag gugacacugc uugggauuuu     1860

ggaucagucg gcggggcauu aaauucauua gguaaaggaa uacaucagau uuuuggagca     1920

gccuuuaaau cauuauuugg ugggaugagu ugguuuucac aaauauuaau aggaaccuua     1980

cuuauguggu uaggcuuaaa uacuaaaaau gguagcauau cacuuaugug cuuagcauua     2040

gguggagucu uaauauuuuu aucuacagca gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4867
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 1 opt

<400>  4867
gggagaaagc uuaccaugac aagcguaggu auaguugguu uacuuuuaac aacagcaaug       60

gcagcagaag uaacuagaag aggaagugca uauuauaugu accuagaucg uaacgacgcg      120

ggugaagcaa ucagcuuucc caccacuuua ggaaugaaua aauguuacau acaaauaaug      180

gacuuaggac auauguguga ugcaacaaug aguuaugaau guccaaugcu agaugaggga      240

guugaaccag augaugucga cuguuggugu aauacaacaa guacuugggu uguauauggg      300

acuugucauc auaagaaagg ugaagcuagg agaucaagac gcgcagugac acuaccuagu      360

cauagcacua gaaaacucca aacacgauca caaacauggu uagaaucacg cgaauacaca      420

aaacacuuga uacguguaga aaacuggaua uuucguaacc cagguuucgc acuggcagca      480

gcagcaauag cauggcuauu aggaaguagc acuucacaaa aagucauaua ucuagucaug      540

aucuuauuaa uagcaccagc auauaguaua agaugcauag ggguaucaaa cagagauuuc      600

guagaaggaa ugaguggagg aacaugggua gacguaguac uggaacacgg aggaugcgua      660

acuguaaugg cacaagacaa accuacagua gauauagagu uaguaacgac uacaguuagu      720

aacauggcag aaguuagaag uuauuguuau gaagcaagua uaagugauau ggcaagcgau      780

aguagaugcc caacccaagg agaagcauau uuagauaaac agagcgacac ucaauauguu      840

uguaaaagaa cuuuaguaga caggggaugg gggaacggau guggacuauu uggaaaagga      900

uccuugguua caugugcaaa auuugcaugu ucaaagaaaa ugacaggaaa aucaauccag      960

ccugaaaacc uugaauacag aaucaugcua agcguacaug ggucacaaca cucaggaaug     1020

auaguaaacg auacgggaca ugaaacagau gaaaacagag caaaagugga aauaacccca     1080

aauagcccac gagcagaagc aacucuugga gguuuuggau cucuaggauu agauugugaa     1140

ccccgaacug gucuagauuu uagugacuua uauuaucuca cuaugaauaa caaacacugg     1200

uuaguacaua aagaaugguu ccaugauaua ccacuacccu ggcaugcugg agcagacacg     1260

ggaacaccac auuggaauaa uaaagaagcu cuaguagaau uuaaagaugc acacgcaaaa     1320

agacagacag uagugguauu aggaucacaa gaaggugcag uacauacagc acuugcagga     1380

gcccuagaag cagaaaugga cggagcaaaa ggccgacuaa guaguggaca ucuaaaaugu     1440

agauuaaaaa uggacaaauu aagacuaaaa ggaguaucuu auucauuaug uacagcggca     1500

uuuacauuua cuaaaauacc cgcugaaaca cuacacggaa ccguaacagu agaacuacaa     1560

uaugcaggua cagauggucc auguaaagua ccugcccaaa uggcuguaga uaugcaaacg     1620

uuaacaccag uuggaagguu aauaaccgca aacccaguaa uaacagaauc aacggaaaac     1680

agcaaaauga uguuagaauu agacccacca uuuggugacu cauauauagu aaucggagua     1740

ggagaaaaga aaauaacaca ucacuggcau agaaguggaa guacuauagg aaaagcauuu     1800

gaagcaacag uaagaggagc aaaaagaaug gccguauuag gagauacugc uugggauuuu     1860

gguucaguag guggagcacu aaacagucua ggaaaaggaa uacaccaaau auuuggugca     1920

gcauuuaaaa gucuauucgg cggaaugucg ugguuuucac aaauacuaau agguacacua     1980

uuaauguggc uuggacuuaa uacuaaaaau ggaagcauca gcuuaaugug uuuagcauua     2040

ggaggcgucc uaauauucuu aaguacagca guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4868
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 1 opt

<400>  4868
gggagaaagc uuaccaugac aagcguaggc auaguaggau uacuauuaac aacagcaaug       60

gcugcagaag uaacaaggcg aggaagugca uauuauaugu auuuagauag aaaugacgca      120

ggcgaagcaa uaaguuuucc aacaacauua ggaaugaaca aauguuacau acaaauaaug      180

gauuuaggac auauguguga cgcaacaaug ucauaugaau guccaauguu agaugaagga      240

guggaaccag augauguaga cuguuggugc aauacuacaa guacgugggu aguauacgga      300

acgugccauc auaaaaaagg agaagcccga agaagccgaa gagcuguaac ucuacccagc      360

cauucuaccc gcaaauuaca aaccagauca caaaccuggu uagaaucaag agaauauaca      420

aaacacuuaa uuagaguuga gaauuggauc uuuagaaauc ccgguuuugc auuagcagca      480

gcggcaaucg cauggcuauu aggaucuuca acaucacaaa aaguaauaua cuuaguaaug      540

auauuauuaa uagcuccagc auacaguaua agauguauag gaguaucaaa uagagauuuc      600

guugaaggaa ugucuggagg aacaugggua gauguaguac uagaacacgg cggauguguu      660

acaguaaugg cacaagauaa accuacagua gauauagagc ucgucacaac uaccguuuca      720

aauauggcag aaguaagguc auauugcuac gaagcaagca ucagugauau ggcaagcgau      780

ucaagauguc caacucaagg agaagcauau cuagacaaac aaucagacac acaauaugua      840

ugcaaaagaa cacuuguaga caggggaugg gguaaugggu gcggccuauu cgguaaaggu      900

ucgcuaguaa caugcgcgaa auuugcaugc aguaaaaaaa ugacagguaa aaguauucaa      960

ccagaaaacu uagaauauag gauaaugcua ucuguucaug gaucacaaca cagcggaaug     1020

auaguaaaug auacuggaca cgaaacagau gaaaaccgag caaaaguaga aauaacacca     1080

aauagcccaa gagcugaagc aacucuaggu ggauuugguu cacuagguuu ggacugugaa     1140

ccaagaacag gacuggauuu uucagaucua uauuauuuga ccaugaacaa caaacauugg     1200

cuuguacaua aagaaugguu ccaugauauc ccauuaccau ggcaugcagg cgcggauaca     1260

ggaacaccac auuggaauaa caaagaagca cuaguggagu ucaaagacgc acacgcaaaa     1320

agacagacag uaguaguacu aggaucacaa gaaggagcag uucauacagc uuuagccggc     1380

gcuuuggaag cagaaaugga uggagcaaaa gguagacuua guuccggcca ucuuaaaugu     1440

cgacugaaaa uggauaaacu acgacuaaaa ggagugucau auucacucug uacagcagca     1500

uuuacuuuca cuaaaauacc agcagaaaca uuacauggaa cuguaacagu agagguacaa     1560

uaugcuggca cagacggccc auguaaagua ccagcacaaa uggcugucga uaugcagacc     1620

uuaacuccug uugguagacu aauaaccgca aauccaguaa uuacagaaag uauagaaaau     1680

agcaaaauga ugcuugaacu agauccacca uuuggugauu cguauauagu uauagggguu     1740

ggagaaaaaa aaauaacuca ucauuggcau agaucaggau caacuauagg aaaagcauuu     1800

gaagcuacag uaagaggagc aaaaaggaug gcaguuuuag gugacacagc augggauuuu     1860

gguucuguag gaggagcauu aaacucccua gguaaaggaa uacaucaaau auuuggugca     1920

gccuuuaaaa gccuauuugg agguaugucu ugguuuucac aaauauuaau aggaacacuu     1980

cuaauguggc uaggacuaaa cacuaagaau ggaaguauca gcuuaaugug uuuagcacuc     2040

ggaggaguac uaauauuuuu aucaacagca guguccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4869
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 1 opt

<400>  4869
gggagaaagc uuaccaugac aucaguugga auaguagguc uauuacuaac aacagcaaug       60

gcugcagaag uaacucggcg uggaagcgcu uauuacaugu acuuagacag aaaugaugca      120

ggcgaagcaa uaucauuucc aacaacacua ggcaugaaua aaugcuacau acaaauaaug      180

gauuuagggc auauguguga ugcaacuaug ucuuacgaau guccaauguu agaugaaggc      240

guagaaccag augauguaga uuguuggugc aauacaacuu caacaugggu aguauacggu      300

acaugucauc acaagaaagg agaagcuaga agaucaagga gagcgguaac acuaccuagu      360

cauaguacaa gaaaacuaca aacucgauca caaaccuggu uagaaucacg ugaauacaca      420

aaacauuuaa uaaggguaga aaauuggaua uucagaaauc cagguuuugc acuggcagca      480

gcugcaauag cauggcuuuu aggaucaagc acaagucaaa aagucauaua ccuggugaug      540

auauuauuaa uagcuccagc uuauaguaua cgauguauag gaguaaguaa uagagauuuc      600

guagaaggaa ugucaggagg uacauggguc gaugucguuc uagaacaugg aggauguguc      660

acgguaaugg cacaagacaa accaacagua gauauugaau ugguaacuac aacuguaagc      720

aacauggcag aaguaagauc auauuguuac gaagcaucua uaagugacau ggcaucagau      780

ucacgaugcc caacacaagg ugaagcauau uuagauaaac aaucugacac ucaauaugua      840

uguaaaagaa cucuaguaga ucgaggaugg ggaaaugguu gugggcuauu uggaaaagga      900

agccuaguaa caugcgcaaa auuugccugu ucaaagaaaa ugacaggaaa aucaauccaa      960

ccagaaaacc ucgaauauag aauaaugcua ucaguacacg gaucacaaca cagcggaaug     1020

auaguaaaug acacaggaca ugaaacagac gaaaacagag caaaaguaga aauaacaccc     1080

aauagcccca gagcugaagc aacauuagga ggauucggaa guuuaggacu ugacugugaa     1140

ccaagaacag gauuagacuu cagcgaccua uauuauuuaa caaugaauaa caaacauugg     1200

cuaguacaca aagaaugguu ucacgauaua ccuuuaccau ggcacgcagg cgcugacacu     1260

gguacuccac acuggaauaa caaagaagca cuuguagaau uuaaagaugc gcaugcaaaa     1320

agacaaacag uagugguacu aggcucucaa gaaggugcag uacauacagc acuagcaggc     1380

gcauuagaag ccgaaaugga uggcguaaaa ggaagacuau caucggggca uuuaaaaugu     1440

cgucuaaaaa uggacaaauu aagacuaaaa ggaguaucgu auucauuaug caccgcagca     1500

uucacauuua caaaaauacc agcagaaaca uuacauggaa caguaacagu agaaguacaa     1560

uaugcaggua cagacggacc auguaaaguu ccagcacaaa uggcagugga caugcaaaca     1620

uuaacaccgg uaggaagauu aauaacugca aauccaguua ucacggaaag uacagaaaau     1680

uccaaaauga uguuagaguu agacccucca uuuggagaua guuauauagu uauugguguu     1740

ggggaaaaga aaauaacuca ucacuggcau agaucaggca guacgauagg aaaagcauuu     1800

gaagcuacag uacgaggcgc caaaagaaug gcaguauuag gagauacagc augggacuuu     1860

gguagcguag guggagcccu aaauucauug ggcaaaggua uucaucaaau auuuggagcu     1920

gcauuuaaaa gccucuucgg uggaaugagu ugguuuucuc aaauacuaau aggaacauua     1980

uuaauguggu uaggacuaaa uacuaaaaau gguucuauua gcuuuaugug uuuggcacua     2040

ggaggagucu ugauauuucu aaguaccgca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4870
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 1 opt

<400>  4870
gggagaaagc uuaccaugac aucuguuggu aucguaggau uauuacucac aacagcaaug       60

gcggccgaag uaacuagaag gggaucagca uauuauaugu aucuagauag aaacgaugca      120

ggagaagcca uaaguuuucc gacaacauua ggcaugaaca aaugcuauau acaaauaaug      180

gauuuagggc auauguguga ugcaaccaug agcuaugaau gcccaauguu agacgaagga      240

guagaaccag acgacguaga uuguuggugu aacacgacaa guacaugggu cguauacgga      300

acuugucauc auaaaaaagg agaagcuagg cguucaagaa gagcaguaac auuaccauca      360

cauucaacac gaaaacuuca aacaagauca caaacauggc uagaaucaag agaauauacu      420

aaacauuuaa uaaggguuga aaacuggaua uuuagaaacc caggauuugc ccuagcugca      480

gcagcaaucg cauggcuacu aggaucaagu acaagccaaa aaguaauaua ucuaguaaug      540

auacuauuaa uagcuccagc auacucgauu agaugcauag guguaucaaa uagagacuuc      600

guagaaggaa uguccggagg aacaugggua gacguagugu uagaacaugg aggauguguu      660

acaguaaugg cacaggacaa accaaccguu gacauagaau uaguuacaac aacaguuagu      720

aacauggcug agguacguuc auauuguuau gaagcaucaa uaucagacau ggcaucagau      780

ucacgauguc ccacucaagg ugaagcauac uuagauaaac aaagcgacac acaauacguu      840

uguaaacgaa cacuaguaga uagaggaugg gggaauggau guggccuauu uggaaaagga      900

agccuaguaa caugugcuaa auuugcaugu aguaaaaaaa ugacaggaaa aagcauucaa      960

ccggaaaacu uagaauauag aauuauguua ucaguacaug guucacaaca uucagggaug     1020

auaguaaaug auaccggaca ugaaacugac gaaaacagag caaaaguaga aauaacuccu     1080

aauaguccaa gagcagaagc cacauuaggg ggauuuggga gucuaggacu agauugcgaa     1140

ccuagaacag gauuagauuu uucagaucua uauuauuuaa caaugaacaa uaaacacugg     1200

cuuguucaua aagaaugguu ucacgacaua ccacuaccau ggcaugcagg agcagauaca     1260

ggaacaccac auuggaacaa uaaagaagca uuaguggaau uuaaagaugc acaugcaaaa     1320

aggcaaacug ucguaguucu agguucccaa gaaggugcug uacauacugc acuagcaggg     1380

gcacucgaag cagaaaugga cggagcuaaa ggaaaacuaa guucagggca ccuaaaaugc     1440

agauuaaaaa uggauaaauu acguuuaaaa ggaguaucau auucauugug cacagcagca     1500

uuuacauuua caaaaauucc ggccgaaacg uuacauggca ccguuacggu agaaguacaa     1560

uacgcaggaa cagaugggcc auguaaagua ccugcccaaa uggcaguuga uaugcaaaca     1620

cugacaccgg uugggagacu aauuacugcu aacccaguaa uaacugaauc aacagaaaau     1680

ucaaaaauga uguuagaauu agauccacca uucggcgaua gcuauauugu aauaggagua     1740

ggugaaaaaa aaauaacaca ccauuggcac aggagcggua guacaaucgg caaagcauuu     1800

gaagcaacug uuagaggagc uaaaagaaug gcaguguugg gcgauacagc augggauuuu     1860

gggaguguag gaggggcacu caacucauua ggaaaaggua uacaucaaau auucggagca     1920

gcuuuuaaau cauuauucgg aggaaugagc ugguuuaguc aaauacuuau aggaacacua     1980

uuaauguggu uaggauuaaa uacaaagaac ggaagcauau cauuaaugug uuuagcacuu     2040

ggaggaguuu uaauauuucu aaguacagcu gucagugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4871
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 1 opt

<400>  4871
gggagaaagc uuaccaugac aagcguagga auuguaggac uacuguuaac aacagcaaug       60

gccgccgaag uaacaagaag aggcagcgca uauuauaugu acuuagauag aaaagaugcu      120

ggagaagcaa ucuccuuucc aacaacauua gguaugaaua aauguuauau acaaauaaug      180

gaucuaggac auauguguga cgcaacaaug ucuuaugaau guccaauguu agaugaagga      240

guagaacccg augauguaga cugcuggugu aauacgacau caacuugggu gguauacgga      300

acaugccacc auaaaaaagg agaagcaagg agaucacgua gagcaguuac acuuccuucu      360

cauucaacua gaaaauuaca aacacgaagc caaacuuggu uagaaagcag agaauauaca      420

aaacauuuaa uaagaguaga aaacuggaua uucagaaauc caggauuugc acucgcagca      480

gcagcaauag cauggcuauu ggggucaagc acaucacaaa agguaauaua cuuaguuaug      540

auauuauuaa uugcaccagc cuauucuaua agauguauag gaguaucuaa cagagauuuu      600

guugaaggua ugucaggcgg aacaugggua gaugugguuc uugaacaugg uggaugugua      660

acaguaaugg cacaagauaa accaacagua gauauagaau uaguaacaac aacaguaagu      720

aacauggcag agguaagaag uuauuguuau gaagcaagua ucucagauau ggcaagcgac      780

ucuagaugcc caacacaagg agaagcauau cuagauaaac aaagugacac acaauaugua      840

uguaaaagaa cacuaguaga uagaggaugg ggaaauggau guggacuuuu uggaaaaggu      900

ucguuaguua cuugcgcaaa auuugcaugu ucaaaaaaaa ugacuggaaa aaguauacaa      960

ccagaaaacu uagaguauag aauaauguua ucaguacacg ggucucaaca uaguggaaug     1020

auuguuaaug acacuggaca ugagacagau gaaaauagag caaaaguaga aauaacucca     1080

aacucacccc gugcagaagc gacauuagga ggauuugguu cauuaggauu agauugugaa     1140

cccaggacgg gacuagauuu uagugaucua uauuaucuaa cuaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ucacgacauc ccauuaccau ggcacgcagg agcagauaca     1260

ggaacaccuc auuggaauaa uaaagaagca uuaguugaau uuaaagacgc ucaugcaaaa     1320

cgucaaaccg uaguuguacu agguagccaa gaaggagcag uacauacugc auuagcagga     1380

gcauuagaag cagaaaugga uggcgcgaaa gguagauuaa gcaguggcca uuuaaaaugu     1440

agacucaaaa uggauaaacu cagauuaaaa ggaguuucau auagucuaug uacagcagca     1500

uuuacauuua caaaaauacc agccgaaaca cuccauggaa ccguaacagu agaagugcaa     1560

uacgcaggaa cagauggacc gugcaaagua ccagcccaaa uggcaguaga uaugcaaaca     1620

uuaacaccug uaggaagauu aauaacagca aauccaguaa uuacagaguc aacagaaaac     1680

agcaaaauga uguuagaacu agauccccca uucggagaua guuauauugu aauaggugua     1740

ggugaaaaaa agaucacaca ucauuggcac agaucaggau caacaauugg gaaagcuuuc     1800

gaagcaacag ucagaggugc aaaacgaaug gcaguacuag gagauacagc gugggacuuc     1860

ggaaguguag guggagcacu aaacucacug gggaaaggaa uacaccaaau auucggagca     1920

gccuuuaaau cacuauuugg gggcaugucu ugguucucuc aaauacuaau uggaaccuua     1980

uuaauguggc uaggacuaaa cacuaaaaau ggaagcauca guuuaaugug ucuagcacuu     2040

ggagguguuu uaauauucuu aucgacagcc guuuccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4872
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 1 opt

<400>  4872
gggagaaagc uuaccaugac aaguguagga auuguaggac uauuauuaac aacagcuaug       60

gcugcagaag uaacaagaag aggcagugca uacuauaugu auuuagauag aaaugacgcu      120

ggagaagcaa uuagcuuccc aacaacauug ggaaugaaua aaugcuacau acaaauaaug      180

gauuuaggcc auauguguga ugcaacaaug aguuaugaau gccccaugcu agacgaagga      240

guugaaccag augauguaga uugcuggugu aauacaacuu caacaugggu uguuuauggu      300

acaugucacc auaaaaaagg agaagcaagg cguucaagac gagccguaac acucccuucg      360

cacucaacuc gaaaauuaca aacccgaagc caaacauggc uagaaucaag agaauauaca      420

aaacaucuga uaagagucga aaacuggaua uuuagaaauc caggauuugc acuagcagca      480

gcagcaaucg cauggcugcu ugggagcuca accucucaaa aagugaucua ucuuguaaug      540

auacuguuga uagccccagc auacucaaua agauguauag guguaagcaa uagagauuuc      600

guagaaggca ugucaggagg aacauggguu gauguaguau uagaacacgg agguugcguu      660

acagugaugg cacaagacaa accaacagua gacauagaac uaguaacaac cacuguaagu      720

aauauggcag aaguacgauc uuauuguuau gaagcuagua uuucagauau ggcaucggau      780

ucaagguguc cuacucaagg ugaggcauac cuagacaagc aaucagacac ucaauacgua      840

ugcaaaagaa cacuaguuga uagaggaugg ggaaauggau guggauuguu cggaaaaggu      900

ucauugguaa caugugcaaa auuugcgugc ucuaaaaaaa ugacagguaa aucuauacaa      960

ccggaaaacu uagaguauag aauaaugcua aguguacaug gcucucaaca uucuggaaug     1020

auuguaaaug auacaggaca ugaaacagau gaaaauagag caaaaguaga aauaacgcca     1080

aauaguccac gagcggaagc aacacuagga ggauuuggua guuuaggauu agacugugaa     1140

ccgagaacag gauuagauuu cagcgaccua uauuauuuaa cgaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ucaugacauu ccccucccau ggcacgcagg ggcagacaca     1260

gggacaccac auuggaacaa uaaagaagca cuaguagaau uuaaagaagc acacgcaaaa     1320

agacaaacag uuguaguacu aggaagucaa gaaggagcag uacauacagc acuagcagga     1380

gcuuuagaag cagaaaugga uggagcaaag ggaagacuau cuucuggaca cuuaaaaugu     1440

agauuaaaaa uggauaaacu gagacuaaaa gguguaucau auucguuaug uacagcagcc     1500

uucacauuua cuaaaauacc agcagaaacu cuacauggaa cgguuacagu agaaguacaa     1560

uaugccggaa ccgauggccc gugcaaagua ccagcacaaa uggcagugga uaugcaaaca     1620

uuaaccccag uaggaagauu aauaacagca aauccaguaa uaacagaauc aacagaaaau     1680

agcaaaauga uguuagaauu agauccaccu uuuggagaua gcuacauagu aauaggaguc     1740

ggagaaaaaa aaauaacuca ccauuggcac agaagcggau caacaauagg aaaagcauuu     1800

gaggccacag uuagaggagc aaaaagaaug gcaguacuag gagacacugc cugggauuuu     1860

ggaagcguug gaggggcauu aaauagucuu gguaaaggga uucaucaaau auucggggca     1920

gcauuuaaau cacuauuugg aggaaugagu ugguucucac aaauauugau aggaacauug     1980

cuaauguggu uaggacuaaa uacaaaaaau ggcagcauaa gccucaugug ucuugcucua     2040

ggaggaguuc uaauauuuuu aaguacugca guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4873
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 1 opt

<400>  4873
gggagaaagc uuaccaugac aaguguugga auaguaggau uacuacuaac uacugcaaug       60

gcagcagaag uaacuagaag aggcucagcu uauuacaugu auuuagacag gaacgaugcc      120

ggcgaagcga uaaguuuucc uaccacacua ggaaugaaca aauguuacau acaaauaaug      180

gauuugggac auauguguga ugcuacgaug agcuaugagu guccaaugcu agacgaagga      240

guugaaccug augacguaga uuguuggugu aacacaacaa gcacaugggu uguauaugga      300

acaugucauc acaaaaaagg agaagcuaga agaucaagaa gagcagucac auuaccaagu      360

cauagcacaa gaaaacuaca aacaagauca caaacauggu uagaaucaag agaauauaca      420

aaacaccuua uaagaguuga aaauuggauu uuuagaaauc caggauuugc uuuagcagcu      480

gcagcaauag caugguuacu cgguucuuca acaagccaaa aaguaaucua ucuaguaaug      540

auauugcuaa uugccccagc guacucuauu cgauguauug gcguaucaaa cagagauuuc      600

gucgaaggaa ugucaggagg cacaugggua gacguuguac uagaacacgg agggugcgua      660

acaguaaugg cacaggacaa acccacaguu gauauagaau uaguaaccac aacgguauca      720

aacauggcgg aaguuagauc uuauuguuau gaggcaagua uaagugauau ggcaucagau      780

aguagauguc caacccaagg ugaagcauac uuagauaaac aaucagacac acaauacgua      840

uguaaaagga cacugguaga cagaggaugg ggaaauggau guggauuauu ugguaaagga      900

aguuuaguaa caugugcaaa auuugcaugu ucaaaaaaaa ugacgggaaa aaguauacaa      960

ccagaaaauu uagaauauag aauuauguua aguguucaug gaagccaaca uagcgguaug     1020

auagugaacg acacagguca cgaaacugau gaaaacaggg cgaaaguaga aauaacacca     1080

aauaguccgc gcgcagaagc agcuuuagga ggauucggau cacuggguuu agauugcgaa     1140

ccaagaacag guuuagauuu cagugacuug uacuaccuaa caaugaauaa uaaacauugg     1200

uuagugcaca aagagugguu ucaugauaua ccauugccau ggcaugccgg agcagauaca     1260

ggcacgccac auuggaauaa uaaagaggcg uuaguugagu ucaaagaugc acaugccaaa     1320

cgacaaacag uaguaguauu aggaucacaa gaaggggcag uucacacagc auuagcagga     1380

gcauuagaag cagaaaugga cggagcuaaa ggaagauuaa gcucaggaca ccuaaaaugu     1440

agauuaaaaa uggauaaguu gcgauuaaaa gguguaucuu acucacuuug uacagcagcu     1500

uucacauuua caaaaauacc cgccgaaaca cuacacggua caguaacagu agagguacaa     1560

uaugccggaa cagauggacc auguaaaguu ccagcacaga uggcaguaga uaugcaaaca     1620

uuaacaccug ucggaagauu aauaaccgca aauccaguga uaaccgaauc uacugaaaau     1680

ucaaaaauga uguuagaauu agacccucca uuuggagauu cauacauagu uauaggagua     1740

ggagagaaaa aaauuacaca ucauuggcau agauccgguu caacuauagg aaaagcauuu     1800

gaagcuacag uaagaggcgc aaaacguaug gcaguauuag gagacacggc augggauuuc     1860

ggaucaguag gaggagcauu aaacucauua ggaaaaggaa uacaccaaau uuucggugcg     1920

gccuuuaaau cauuauuugg uggaaugagc ugguuuucgc aaacauuaau aggcacuuua     1980

cucauguggu ugggacuuaa uacaaaaaau ggaagcauau cacucaugug uuuagcacug     2040

ggaggaguac uuauauuuuu auccacugca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4874
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 1 opt

<400>  4874
gggagaaagc uuaccaugac aaguguaggu auuguaggac uuuuauuaac uacagcaaug       60

gcagcagagg uuacuagaag aggaucugcg uauuauaugu aucuagacag aaaugacgca      120

ggagaagcaa uaucuuuucc uacgacauua ggaaugaaua aaugcuauau acaaauaaug      180

gauuuaggcc acaugugcga ugcaaccaug ucauaugaau guccaauguu agaugaagga      240

guugaaccag augauguaga uugcuggugu aauacaacau caaccugggu aguauaugga      300

accugucacc acaaaaaagg cgaagcaaga agaaguagaa gagcaguaac auuaccauca      360

cauagcacac gaaaacuuca aacacgaagc caaacauggc uugaaaguag agaauauacu      420

aaacaucuaa uaagaguaga aaacuggaua uucagaaauc caggcuuugc auuagcagca      480

gcugccauug cauggcuauu aggcaguuca acaucacaaa aaguaauuua uuuaguaaug      540

auucuauuaa uugcaccugc auauaguaua aggugcauag gaguuuccaa uagagauuuu      600

guagaaggaa ugucaggagg uacaugggua gauguaguuc uagaacaugg ugguugugua      660

acaguaaugg cucaggauaa accaacagua gauauagaac uaguaacgac aacaguaagc      720

aacauggcag aaguuagaag cuauugcuac gaagcaagua uaucagauau ggcaagcgau      780

ucacgauguc caacacaagg agaagcuuac cuugacaaac aaucugauac ucaauaugua      840

ugcaaaagga cguuaguaga ucgugguugg ggaaaugguu guggcuuauu cggaaaagga      900

agucuaguua caugcgccaa auuugcaugu aguaaaaaga ugacagguaa aucaauacaa      960

ccagaaaauu uagaauaucg aaucauguua ucaguacaug guucacaaca uucugguaug     1020

auuguaaaug acacaggaca ugaaacagac gaaaauagag caaaaguaga aaucacacca     1080

aacucuccaa gagcugaagc aacuuuagga ggguucggcu cguuaggauu agauugugaa     1140

ccaagaacgg gacuagacuu uucagaucua uauuauuuga caaugaauaa uaaacacugg     1200

cuaguucaua aagaaugguu ucaugauaua ccacuaccau ggcaugccgg agcagacacu     1260

ggaacccccc auuggaauaa caaagaagca cuaguagaau uuaaagaugc ucacgcgaaa     1320

cgacaaacug uaguaguacu uggaucacaa gaaggagcag uacacacagc auuagcagga     1380

gcuuuagaag cugagaugga cggcgcuaaa ggacgacuaa guucaggcca uuuaaaaugc     1440

agauuaaaaa uggauaaacu aagauuaaaa ggaguuucau auucacuaug uacagcagcu     1500

uuuacauuua ccaaaauacc ugcagaaaca uugcauggaa caguuacagu agaaguacaa     1560

uacgcuggag cugauggacc uuguaaagua cccgcucaaa uggcaguaga uaugcagaca     1620

uuaacaccag ucggaagauu aauuacagcc aauccaguaa uuacagaauc aacggaaaau     1680

ucaaaaauga uguuagagcu agacccgcca uuuggagauu cauauauugu gaucggugua     1740

ggcgaaaaaa aaauuacaca ccauuggcau agaucagguu cuacaauagg caaagcguuu     1800

gaagcaacug ugaggggcgc aaaaagaaug gcaguauuag gagauacugc augggauuuc     1860

ggaaguguag gaggagcacu aaacucccug ggaaaaggua uccaucaaau auuuggagcg     1920

gcguucaaau cauuauucgg uggaauguca ugguucucac aaauccucau aggaacuuua     1980

uuaauguggu uaggacuuaa uacaaaaaau ggcaguauau cauuaaugug ccuagcacua     2040

ggaggagucu uaauauuuuu aaguacugca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4875
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 1 opt

<400>  4875
gggagaaagc uuaccaugac aucaguggga auaguagguu uacuacuaac aacagcaaug       60

gcagcagaag uaacaagaag aggaucugca uauuauaugu auuuggauag aaacgaugca      120

ggggaagcaa ucaguuuucc uacaacguug ggaaugaaua aauguuauau acaaauaaug      180

gaccuaggac auauguguga ugcuacaaug ucguaugaau gcccgaugcu ggaugaaggg      240

guagaaccag augauguaga cuguuggugu aauacaacca guacaugggu gguauaugga      300

acaugucauc auaaaaaagg ugaggcucga cgaaguagga gagccguaac auuaccuuca      360

cauucaacaa gaaaauuaca aacaagguca caaacauggu uagaaaguag agaauauaca      420

aaacaucuaa uuagaguuga aaacuggaua uuuagaaacc caggguucgc uuuggcagca      480

gcagcaauag caugguuauu aggaagcuca acaucucaaa aaguuauaua ucuagucaug      540

auauuacuua uagcauuagc uuauuccaua cgauguaucg gaguaucaaa uagagauuuc      600

guagaaggaa ugucaggagg aacauggguu gacguagugu uagaacaugg uggcugugua      660

acaguaaugg cacaagauaa gccaacagua gacauugaau uaguaacuac aacaguaagu      720

aauauggcag aaguaagauc uuauuguuac gaagcaagua ucagcgauau ggccuccgau      780

ucaagaugcc cgacacaagg ugaagcauau uuagauaaac aaagcgauac gcaauacgua      840

uguaaacgua ccuugguaga cagagguugg gguaauggau gcggauuauu uggaaaaggc      900

ucacuuguua cgugugcaaa auucgcaugc ucaaaaaaaa ugacuggaaa auccauacaa      960

ccagaaaauc uagaauauag aaucaugcua uccguacaug gaucccaaca uucagguaug     1020

aucguaaaug auacagggca ugaaacagau gaaaauagag cgaaaguaga aauaacccca     1080

aauaguccaa gagcugaggc aacuuuagga ggauuuggau cacuaggcuu agacugcgaa     1140

ccgaggacag guuuagauuu uagugaucua uauuaucuua cuaugaauaa uaaacauugg     1200

uugguacaua aagaaugguu ucacgauaua ccauuaccau ggcaugcagg agcugauaca     1260

ggaacaccac auuggaauaa uaaagaagcc uugguugaau uuaaagacgc acaugcaaaa     1320

cgacaaacag uagugguauu aggaagucaa gaaggggccg ugcacacagc auuagcugga     1380

gcucuagaag cagaaaugga uggagcaaaa ggaagacuau caucaggaca ccuaaaaugc     1440

cgguuaaaaa uggacaaacu aagacuaaaa ggggugaguu acucauugug uacugcagca     1500

uuuacauuca caaagauacc agcagaaacc uuacauggua cugucacagu agaaguacaa     1560

uaugcaggaa cagauggacc auguaaagua ccugcacaaa uggcaguaga caugcagaca     1620

uuaacaccag uagguagacu aauaacugca aaucccguua uaaccgaauc aaccgaaaau     1680

aguaaaauga uguuagaauu agauccaccu uuuggggauu cauauauagu cauaggagua     1740

ggagaaaaaa aaaucacuca ucauuggcac agaagcggau caacaaucgg gaaagcauuc     1800

gaagccacag uuagaggagc aaaaagaaug gcagucuuag gagauacggc cugggauuuu     1860

ggaaguguag ggggggcauu aaauucauug ggaaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaaa guuuguucgg uggaauguca ugguuuucuc aaauacuaau aggaacucua     1980

uuaauguggc uaggucuuaa uacaaaaaau gggaguauau cauuaaugug ccuagcauua     2040

gguggagucu ugauauuccu aagcacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4876
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 1 opt

<400>  4876
gggagaaagc uuaccaugac aaguguagga auaguuggau uacucuuaac aacagcaaug       60

gcagcagaag uaacaagaag ggguucagca uauuauaugu aucuagaucg aaaugaugca      120

ggagaagcaa uaaguuuucc cacaacuuug ggaaugaaca aauguuauau acaaauaaug      180

gacuugggac auauguguga ugcuacuaug uccuaugaau guccaauguu agacgaagga      240

guagaacccg acgauguuga uugcuggugc aacacaacaa guacuugggu uguauacgga      300

acaugccauc acaagaaagg agaagcaaga agaucaagac gagcaguaac uuuaccaagu      360

cauaguacaa gaaaauuaca aacacgauca cagacauggc ucgaaucaag ggaauauaca      420

aaacaucuaa uuagaguaga aaauuggaua uucagaaauc caggauuugc auuagcagcu      480

gcagcaaucg cauggcuuuu agguucaucg acuucacaaa aaguuauaua uuuaguaaug      540

auacuucuaa uagcccccgc auauucaauu cggugcauug gagucucgaa ucgagacuuu      600

guggaaggaa ugucaggugg aacaugggua gauguagucu uagaacaugg aggaugcguu      660

acuguaaugg cucaagauaa accuacagua gauauagagc uaguaacaac uacaguaagu      720

aauauggcag aaguaagaag uuauuguuau gaggcaagua uaucugauau gguaucagau      780

ucaagauguc caacacaggg agaagcauac cuagauaaac agucagauac ucaauauguu      840

uguaaaagaa cauuaguaga ucgaggaugg gguaauggcu guggauuauu uggaaaagga      900

ucacuuguaa caugugcgaa auuugcaugu ucaaagaaaa ugacaggcaa aucaauucaa      960

ccagaaaauu uagaauacag aauaaugcua aguguacacg guucacaaca uuccggcaug     1020

auaguaaacg acacuggaca ugaaacugac gaaaauagag caaaaguuga aauaacacca     1080

aauaguccua gagcagaagc aacauuaggu ggauuuggau cauugggauu agauugugaa     1140

cccagaacag gucuggauuu uucagauuua uauuaccuua ccaugaacaa uaaacauugg     1200

cuagugcaua aagaaugguu ccacgacaua ccacuaccuu ggcaugcagg agcagauaca     1260

ggaacaccac auuggaauaa uaaagaggcu uuaguugaau uuaaagacgc acaugcuaaa     1320

agacaaacag uaguaguauu aggaagucaa gaaggagcag uacauacagc acuagcuggu     1380

gcauuagaag cagaaaugga cggugcaaaa gguagauuau caucaggaca cuuaaaaugu     1440

agauuaaaaa uggauaaacu acgccuaaaa gguguaucau auucucuaug uacagcagca     1500

uuuacauuca caaaaauucc ugcagaaaca uuacauggua caguaacagu agaaguacag     1560

uaugcaggaa cggauggacc cuguaaagua ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccag ucggaagauu aauuacugcc aacccaguaa uuacagaaag uacagaaaac     1680

agcaaaauga uguuagaacu agacccaccc uuuggagacu cuuauauagu aauaggugua     1740

ggagaaaaga aaauaacaca ucauuggcau cguaguggua gcacaauagg aaaagcguuc     1800

gaagcaacag uaagaggagc aaaaagaaug gcuguauuag gugauacagc uugggauuuc     1860

ggauccguag gaggagcacu caacaguuua ggaaaaggua uucaccaaau cuuuggagca     1920

gcauuuaaaa guuuauuugg aggaaugagc ugguuuaguc aaauauuaau ugguacucua     1980

uuaauguggu uaggauuaaa uacuaaaaau ggaucaauaa gucuaaugug ccuagcauua     2040

ggcgguguac uaauauuucu gucuacagcg guuucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4877
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 1 opt

<400>  4877
gggagaaagc uuaccaugac aucaguuggg auuguggguc uucuuuuaac aacagcuaug       60

gcagcagaag uaacaagaag aggaucagca uacuacaugu auuuagaucg aaaugaugcu      120

ggagaagcaa uaaguuuucc aacuacuuua ggaaugaaua aauguuauau acaaauuaug      180

gauuuaggac auauguguga ugcuacuaug agcuaugaau guccaaugcu agaugaagga      240

guagaacccg acgauguaga uugcuggugu aacacuacaa gcacgugggu uguauaugga      300

acuugccacc auaagaaagg agaagcuaga agaaguagac gagcaguaac acuaccuagu      360

cauucaacga gaaaacuaca aaccagauca caaacauggc uagaaucaag agaauauaca      420

aaacaccuca uaagaguuga aaacuggaua uuuaggaacc caggauuugc ucuagcagca      480

gcugcaaucg caugguuauu aggauccuca acaucacaaa aaguuauaua cuuaguaaug      540

auuuuauuaa uagcaccagc uuauuccauu agauguauag guauaagcaa cagggacuuu      600

guagaaggaa ugaguggagg aacuuggguc gauguagucu uagaacacgg cggaugcgua      660

acuguaaugg cacaggauaa accaacugua gauauagaau uaguaaccac aacuguaucu      720

aauauggcug aaguaagauc auacugcuau gaagcaagua uaucugauau ggcaucagac      780

ucaagaugcc caacacaagg agaagccuau uuagacaaac aaagugauac acaauacgua      840

uguaaaagga cauuaguaga uagaggaugg ggaaauggcu gcggauuauu ugguaaagga      900

agucuaguaa cuugugccaa auucgccugu ucaaagaaaa ugacaggaaa aucaauucaa      960

cccgaaaauc ucgaauaucg uauaauguug ucaguacacg gaucacaaca uaguggaaug     1020

auaguuaacg acacuggcca cgaaacggac gaaaaucgag caaaaguaga aauaacacca     1080

aauaguccua gagcagaagc gacauuagga ggauuuggca uauuaggauu agauugugaa     1140

ccaagaacug gacucgauuu uucagauuua uacuauuuga caaugaauaa uaaacacugg     1200

uuaguacaca aagaaugguu ucaugacaua ccuuuaccgu ggcaugcagg agcagacaca     1260

ggaacgccuc acuggaacaa uaaagaagca uuaguggagu ucaaagaugc acacgcaaaa     1320

agacaaacag uaguaguauu agguagucaa gaaggagcag uacauaccgc acuagcuggc     1380

gcuuuagaag cagaaaugga uggcgcaaaa ggaagauuau caagcggaca uuuaaaaugu     1440

aggcuaaaaa uggauaaacu aagauuaaaa ggaguaagcu auagcuuaug cacagcagca     1500

uucacauuua caaaaauacc agcagagaca uuacauggua caguuacugu agaaguccaa     1560

uaugcaggua cugauggacc auguaaagua ccggcacaaa uggcaguaga caugcaaaca     1620

uuaacaccug uugguagacu aaucacagca aauccuguaa uaacagaauc aacagaaaac     1680

ucgaaaauga uguuagaauu ggacccucca uuuggagacu cauauauugu cauaggagua     1740

ggagaaaaga aaauaacuca ucauuggcau agaucaggau caacaauagg aaaagcauuu     1800

gaagcaacag ugagaggagc caaaagaaug gcgguucuug gagacacagc uugggauuuu     1860

ggaucuguag gaggagcauu aaauaguuua ggaaaaggaa uacaucaaau auuuggcgcc     1920

gcuuuuaaaa gccuuuuugg aggaauguca ugguuuaguc aaauacugau agguacgcua     1980

uuaauguggc uugguuugaa uacgaaaaau ggaaguauaa guuuaaugug ucuggcacuu     2040

ggagggguau uaauauucuu aucaacagca gugucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4878
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 1 opt

<400>  4878
gggagaaagc uuaccaugac caguguaggg auaguaggau uacuauuaac uaccgcaaug       60

gccgccgaag uaacaagaag aggcagugca uauuacaugu acuuagacag gaaugacgcc      120

ggugaagcua uaaguuuucc aacaacuuua ggaaugaaua agugcuauau ucaaauaaug      180

gacuuaggac auacguguga ugcaacaaug ucauaugaau guccgauguu ggacgaagga      240

guagaaccag augauguaga uuguuggugu aacacaacga guacaugggu uguauaugga      300

acuugucauc acaagaaagg agaagcaaga agaagcagac gagcaguaac ccuucccucu      360

cacaguauaa gaaaacuaca aacaaggagc caaacauggc uagaaaguag agaauauaca      420

aaacaucuga uuagaguuga aaauuggaua uucagaaauc cuggauuugc guuagcagcg      480

gcagcaauag caugguuauu agguaguuca acaagucaaa aaguaauuua ccuaguaaug      540

auauuacuaa uugcaccagc auacucuaua aggugcaucg gaguaucaaa uagagauuuc      600

guagaaggua ugucaggagg aacaugggua gacguagugu uagaacaugg uggaugugua      660

acuguaaugg cgcaagauaa accaacagug gauauagaac ugguaacaac uacuguaagc      720

aauauggcag aaguacgauc guauuguuau gaggccagua uaucugauau ggccagugac      780

aguagaugcc caacacaagg ugaagcauac cuagacaaac aaucagacac ucaguauguu      840

uguaaacgaa cauuaguaga ucgagguugg gguaauggau guggguuauu cggaaaagga      900

aguuuaguaa cuugugcaaa auuugcaugu ucaaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaauc uagaauauag aauaauguua ucuguacaug guagccaaca cagcggaaug     1020

auuguuaacg acacaggaca ugaaacggau gaaaauagag cgaaaguuga aauaacccca     1080

aauagcccaa gagcagaagc aacuuuaggu ggauuuggau cauuagguuu agauugugaa     1140

ccaagaaccg gacuagacuu uagugaccua uauuauuuaa caaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ucacgauaua ccacucccau ggcacgccgg agcagauaca     1260

gguacaccac acuggaauaa uaaagaagca uuaguagagu ucaaagaugc gcacgcaaaa     1320

agacaaacug uugugguucu aggaagucaa gaaggcgcag uucauacugc auuagcggga     1380

gcacuagagg ccgaaaugga cggagcaaaa ggaagacuua gcucagguca ucuaaaaugc     1440

agauuaaaaa uggauaaacu ccgauuaaaa ggaguaucau acaguuuaug uacagcagca     1500

uuuacauuua caaaaauacc agcagaaacu cuacauggaa cugugacagu agaaguacaa     1560

uacgcgggua cugacggacc uuguaaagua ccagcucaaa uggccgugga uaugcaaaca     1620

uuaacaccag uuggaagauu aauuacagcu aacccaguaa uaacugaauc aacagaaaac     1680

agcaaaauga uguuagaauu agacccgcca uuuggagaca guuauauagu aauuggagua     1740

ggcgaaaaga aaauaacuca ucacuggcac cgaucaggau caacaauagg uaaagcauuu     1800

gaagcaacug uucgaggagc aaaaaggaug gcaguacuag gcgauacagc gugggauuuu     1860

ggaucuguag gaggugccuu aaauucuuua ggaaaaggua uacaccaaau uuuuggagca     1920

gcauuuaaau cacuauuugg uggaaugagc ugguucucac aaauauuaau aggaacacua     1980

uuaauguggc uuggucuaaa ugcuaaaaau gguaguauaa guuuaaugug uuuagcauua     2040

ggaggaguac uaaucuuccu aagcaccgca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4879
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 1 opt

<400>  4879
gggagaaagc uuaccaugac auccguaggu auaguaggau uacuacuaac aacagccaug       60

gccgcagaag uaacccgucg aggaucugca uacuauaugu auuuagaccg aaacgacgca      120

ggagaagcua uauccuuccc aacaacucua ggaaugaaua aauguuauau ucaaauaaug      180

gauuuaggac auaugugcga ugcaaccaug ucauaugaau guccaauguu agaugaaggg      240

guagaaccag augauguaga cugcuggugu aauacaacuu ccacaugggu uguauacgga      300

acgugucauc auaagaaagg agaagcgcaa agaaguagac gagcaguuac acuaccaagu      360

cauaguacaa gaaaauuaca aaccagaucu caaacauggc uagaaucaag ggaauacacu      420

aaacaucuaa uaagaguaga aaauuggauu uuuagaaauc cuggauuugc acuagcggca      480

gccgcaauag cauggcuauu aggaaguucu acaucacaaa aaguaauaua cuuaguaaug      540

auacuauuaa uagcaccagc uuacaguauc agaugcauag gaguuucaaa cagagauuuc      600

guagaaggca ugaguggagg aacauggguu gauguaguac uagaacaugg uggaugcgua      660

acaguaaugg cacaagacaa accaacagua gauauugaau uaguaacaac aaccguauca      720

aauauggcug aagucagaag uuacuguuau gaagcuagca uaagugauau ggcaagugau      780

ucaaggugcc caacacaagg ugaggcuuau cuagacaaac aaagugacac acaauaugua      840

uguaaaagaa cuuuaguaga ccgaggaugg ggaaauggau guggauuauu cggaaaagga      900

uccuuaguaa cuugcgcuaa auuugcgugu ucaaagaaaa ugacagguaa aaguauacaa      960

ccagaaaauu uagaauauag aauaaugcua ucaguacacg gaagccaaca uucaggcaug     1020

auaguaaacg auaccggaca ugaaaccgac gaaaacagag caaaaguuga aauuacaccc     1080

aacagccccc gagcagaagc aacccuugga gguuuuggca gcuuaggauu agauugugaa     1140

ccaagaaccg gauuagauuu uagugaccua uauuaucuaa caaugaauaa uaaacauugg     1200

uuaguacaca aagaaugguu ucacgacaua ccauuaccau ggcaugcagg agcagacacu     1260

ggaacaccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc ucaugcaaaa     1320

cgacaaacag uaguaguauu aggcagucaa gaaggggccg uacauacagc cuuagcaggg     1380

gcacucgaag cagaaaugga cggagcuaaa ggaagacuau caagugguca uuuaaaaugu     1440

cgcuuaaaga uggauaaauu gagauuaaaa ggaguaaguu auucacuaug uacagcugca     1500

uuuacauuca caaaaauacc agcagaaaca cuucauggaa ccguaacagu agaaguacaa     1560

uaugcaggga cagauggacc auguaaagua ccugcacaaa uggcaguuga uaugcaaacc     1620

uuaacaccag uaggaagacu aauaacagca aauccaguaa uaacagaauc gacagaaaau     1680

aguaaaauga uguuagaauu agacccaccc uuuggagacu cauauauagu gauaggugua     1740

ggugaaaaga aaauaacuca ccauuggcau agauccggaa guacaauagg aaaagcauuu     1800

gaagcaacag uaagaggagc gaaacguaug gcuguacuag gagauacagc augggauuuu     1860

ggaucaguag gaggagcacu aaauagccuu ggaaaaggaa uacaccagau auuuggagca     1920

gcauuuaaau cuuuauuugg aggaauguca ugguucaguc aaauauuaau agguacauua     1980

cuaauguggu uagguuuaaa cacuaaaaau ggaucuauau cuuuaaugug uuuagcauua     2040

gggggaguuu uaauauuucu aucaacagcu guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4880
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 1 opt

<400>  4880
gggagaaagc uuaccaugac uuccguaggu auaguagguu uacuauuaac aacugccaug       60

gccgcagaag uaacuagaag agguagcgca uauuauaugu acuuagaucg aaacgaugcc      120

ggagaagcaa uaucauuccc aacaacauua ggcaugaaua aauguuauau acaaauaaug      180

gauuuaggac auauguguga ugcaacaaug ucauacgaau guccgaugcu agacgaaggu      240

guagaaccag acgauguaga cuguuggugu aacacaacuu ccacaugggu uguuuaugga      300

acuugucauc auaaaaaagg agaagcaaga cguucacgua gagcuguaac ccuaccauca      360

cauucaacaa gaaaacuuca agcaagauca caaacauggc uagagucuag agaauacaca      420

aaacauuuaa uaagaguaga aaacuggaua uuuagaaauc cgggauucgc uuuagcugca      480

gcagcaaucg ccuggcuauu agguucaagc acaagucaaa aaguaauaua cuuagucaug      540

auauuauuga uagccccagc uuauucaaua agauguauag gaguuucaaa cagggauuuu      600

gucgaaggaa ugucaggagg aacaugggua gaugucguau uagaacacgg aggaugugua      660

acaguaaugg cacaagauaa accaacaguu gacaucgaau uaguuacaac gacaguaagc      720

aauauggcug aaguaagauc uuauuguuau gaagcaagca uuagugacau ggcaagugau      780

ucaaggugcc caacacaagg agaagcuuau uuagacaaac aauccgauac ucaauacgua      840

uguaaaagaa cauuagugga uagaggaugg ggcaauggau guggccuauu ugguaaagga      900

aguuuaguaa cuugugcaaa auuugcaugu aguaaaaaaa ugacagguaa gagcauacaa      960

ccagaaaauc uagaauaucg aauaauguua ucuguucaug gcagucaaca cucaggaaug     1020

auaguaaaug acacaggaca ugaaacugau gagaauagag caaagguaga aauaacacca     1080

aacagcccaa gagcugaagc aacauuagga ggguuuggau cauuaggacu cgauugugaa     1140

ccaaggacug gcuuagacuu uucagauuua uauuauuuaa caaugaauaa uaaacauugg     1200

cuaguacaca aagaaugguu ucaugauauu ccgcuaccau ggcacgcagg agcagauacg     1260

ggaacucccc auuggaauaa uaaagaagcc uuaguagagu ucaaagacgc acacgcaaaa     1320

agacaaacag ucguaguacu uggaagucaa gaaggagcag uacauacagc uuuagcaggg     1380

gcucuugaag cagagaugga cggagcaaaa ggaagacugu caagcggaca ucuaaaaugc     1440

agauuaaaaa uggauaaacu aagauuaaaa ggaguaucuu acagucuuug caccgcugcu     1500

uuuaccuuua caaaaauacc agcagaaacu uuacauggaa cuguuaccgu agaaguccag     1560

uacgcuggaa cagauggccc augcaaagua cccgcacaga uggcuguaga uaugcaaaca     1620

uuaacaccag uagguaggcu gauaacagca aaucccguaa uaacagaauc aacagaaaau     1680

ucaaaaauga ugcuagaacu ugacccaccu uuuggcgaua guuauauagu uauaggagua     1740

ggagaaaaaa aaauaacaca ucauuggcac agaucugguu ccacgaucgg aaaagccuuu     1800

gaagcaacag uaaggggagc gaaaagaaug gcuguacuag gugacacagc cugggauuuc     1860

ggaucaguug gaggugcacu aaauucauua ggaaaaggaa uacaccaaau uuuuggagca     1920

gcauuuaaau cauuauucgg cggaaugagc ugguuuucac agauuuuaau aggaacacua     1980

uuaauguggc uaggacuaaa uacaaaaaau ggaucgauaa gucuaaugug ucuagcacua     2040

ggaggaguau ugauauuucu aucaacggca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4881
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 1 opt

<400>  4881
gggagaaagc uuaccaugac aucaguaggc auaguaggac uauuacuaac aacagccaug       60

gcagcggaag uaacaaggag aggaucugca uauuacaugu acuuagaucg auccgaugca      120

ggagaagcaa uaaguuuucc aacaacuuua gguaugaaua aauguuauau acaaauaaug      180

gauuuaggac acauguguga ugcuacuaug ucuuaugaau gcccaauguu agacgaagga      240

guggaaccug augauguuga uuguuggugu aauacaacaa guacaugggu aguauauggu      300

acaugucauc auaagaaagg agaagcgagg agaucaagga gagcuguaac auuaccaagu      360

cauagcacaa gaaaauuaca aacaagaagc caaacuuggu uagaaaguag agaauacaca      420

aagcauuuaa uaagaguaga aaauuggaua uuuagaaacc caggauuugc acuagcagca      480

gcagcaauag cauggcuauu aggcaguagu accucacaaa aagucauaua uuuaguuaug      540

auacuuuuaa uagccccugc auauagcauu agauguauag gcguuagcaa ucgagauuuu      600

guagaaggua ugucaggagg uacuugggua gauguaguau uagaacacgg gggauguguc      660

accguaaugg cccaagacaa accaacugua gauauagagu uaguuacuac aacaguaagc      720

aauauggcag aagucagaag uuauugcuac gaagcaagca uaagcgauau ggcuagugau      780

agccgauguc cuacccaagg agaggcguau uuagauaaac aaucugacac gcaauaugua      840

uguaaaagaa cucuaguaga cagaggaugg ggcaacggau gcggauuauu uggaaaaggu      900

agcuuaguaa caugugcaaa auuugcaugu ucaaagaaaa ugacuggaaa aaguauucaa      960

ccagaaaacu uagaauaucg aauaaugcua ucaguacaug gaagucaaca uucaggaaug     1020

auaguaaacg acacuggaca cgagacagau gaaaaucgag cgaaaguaga aauaacacca     1080

aauucaccua gagcugaagc aacauuaggu ggauuuggua gcuuaggauu agauugugaa     1140

ccaagaaccg gucuagauuu cagcgaccua uauuauuuaa caaugaauaa uaaacacugg     1200

cuaguacaua aagaaugguu ucaugauauu ccucuaccuu ggcacgcugg agccgacacu     1260

ggaacaccuc auuggaacaa uaaagaagcg uuagucgagu ucaaagaugc ccacgcaaaa     1320

agacaaacug uaguaguauu aggaucacaa gaaggagcag uacauacagc acuagcagga     1380

gcacuagaag cagaaaugga uggagcaaag ggaagauuaa gcaguggaca ccuaaaaugu     1440

agacuaaaaa uggacaaacu aagauuaaaa ggaguuucuu auagucuaug cacggcagcu     1500

uucacauuua guaaaauucc agcugaaaca uuacauggca caguaacaga agaaguccaa     1560

uaugcaggua cugauggacc auguaaagua ccagcacaaa uggcaguaga uaugcagaca     1620

cuaacacccg uagguaggcu uauaacagcg aauccaguaa uaacugaaag uacugaaaau     1680

aguaaaauga uguuagaauu agauccccca uuuggugauu cauacauagu aauaggagua     1740

ggagaaaaga aaauuacaca ucauuggcac cguucaggau caacaaucgg aaaagccuuu     1800

gaagcaacug uuagaggagc aaaacgaaug gcaguuuuag gagauacugc augggauuuc     1860

ggaaguguag gaggggcauu gaauagcuug ggaaaaggua uucaucaaau auucggagca     1920

gcauuuaaaa gucuauuugg ugguaugagu ugguuuaguc aaauacuaau uggaacauua     1980

cuaauguggc uagguuuaaa uacgaaaaac ggaucaauca guuuaaugug ccuagcucua     2040

ggcggcgucc uaauauuuuu gaguacagca guuuccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4882
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 1 opt

<400>  4882
gggagaaagc uuaccaugac aucuguagga auaguaggac uacuacuaac uacagccaug       60

gcugcagagg uaacaagaag aggaucagca uauuauaugu auuuggauag gucagaugcc      120

ggugaagcaa uaaguuuucc uacuacauua ggaaugaaca aauguuauau acaaauaaug      180

gauuuaggac auauguguga ugcaacaaug aguuaugaau gcccgaugcu agaugaagga      240

gucgaaccag augaugucga uuguuggugc aacacaacau caacgugggu ugucuaugga      300

acaugucacc acaagaaagg ugaagcuaga agaucaagga gagcaguuac auugcccagu      360

cauaguacaa gaaaauuaca aacaagaagu caaacauggu uagaaaguag agaauauacu      420

aaacaucuaa uacgugugga aaauuggaua uuuagaaauc caggauuugc gcuagcagca      480

gcagcaauag caugguuacu agguucuagu acaagucaaa aaguaauaua ucuagugaug      540

auauuauuaa uugcacccgc auauaguaua aggugcauag gugucagcaa uagagauuuu      600

guggaaggaa ugucuggagg aaccuggguu gauguaguuu uagaacaugg gggaugcguu      660

acaguaaugg cacaggauaa acccacaguu gauaucgaau ugguuacgac aacaguuagu      720

aacauggcag aaguaagauc auacuguuau gaagcaagua uaucagauau ggcaucagau      780

ucaagauguc caacccaagg agaagcauau uuagacaaac aaagugauac ccaauacgua      840

uguaaacgaa cucuuguaga uagaggaugg ggaaacggau guggauuauu uggaaaaggu      900

agucuaguaa cuugcgcaaa auuugcuugu ucaaagaaaa ugaccggaaa aucaauacaa      960

ccagaaaacu uagaauauag aauuaugcua aguguacaug gaucucagca cagcgggaug     1020

auaguuaacg acacagguca ugaaacagac gaaaauagag caaaaguaga aauaacacca     1080

aacaguccaa gagcagaagc aacccuuggu ggauuuggga guuuaggucu ggauugcgaa     1140

ccgagaacag gauuagauuu uagugauuua uauuaucuaa caaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ccaugauaua ccacuuccau ggcauacggg agcagacacc     1260

ggaaccccac auuggaacaa uaaagaggca uuaguugaau uuaaagacgc acacgcaaaa     1320

agacaaacag uaguaguacu gggcucccaa gaaggagcag uacauacagc acuagcgggu     1380

gcacucgaag cagaaaugga uggagcgaaa ggaagauuaa gcaguggcca ucuaaaaugu     1440

agguugaaaa uggacaaacu aagguuaaaa ggaguaaguu acagccucug uacagcugcu     1500

uucaccuuua caaaaauacc ggcagaaaca uuacacggaa caguaacagu agagguucaa     1560

uaugcaggaa cagauggacc auguaagguu ccagcacaaa uggcagucga uaugcaaaca     1620

uuaacuccag uuggaagauu aauaacagca aauccuguua uaacagaagg aacagaaaac     1680

ucaaaaauga uguuagaacu agauccacca uuuggcgaua guuauaucgu aauaggagua     1740

ggggaaaaga aaauaacuca ucacuggcau agaagcggau caaccauagg uaaagcauuu     1800

gaagcaacgg uaaggggugc aaaaagaaug gcaguauugg gagacacagc uugggacuuu     1860

ggaaguguag gagguguauu aaacagucua ggaaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaaa guuuauuugg aggaaugagc ugguuuucac aaauauuaau aggaacauua     1980

cuuauguggu ugggacuaaa uacuaaaaau ggaucgauaa gccugaugug uuuagcacua     2040

gguggaguuu uaaucuuccu aaguacagca guaagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4883
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 1 opt

<400>  4883
gggagaaagc uuaccaugac aucaguagga auaguaggau uacuacuaac cacagcaaug       60

gcagcagagg uaacaaggag aggaucagca uauuauaugu auuuagaucg aucagacgca      120

ggagaagcaa uaaguuuucc aaccacgcua ggcaugaaua aauguuacau ucaaauaaug      180

gacuuagguc auauguguga ugcaacaaug uccuaugaau guccuauguu agaugaagga      240

guugaaccug augauguuga uuguuggugu aacacaacau caacuugggu aguauauggu      300

acaugucacc auaaaaaagg agaagcuaga cgaaguagaa gagcuguaac acuaccaagc      360

cacucgacua gaaaacuaca aacaagauca caaacauggu uagaaaguag agaauacaca      420

aaacaucuua uacgaguaga gaauuggaua uuucgcaauc caggauuugc acuagcagca      480

gcagcaauag cgugguuauu agguucaucu acaagccaaa aaguaauaua uuuaguaaug      540

auauuauuaa uagcaccagc guauucaaua agauguauag gagucaguaa uagggauuuc      600

guagaaggaa ugucuggcgg aacaugggua gauguaguac uagaacaugg aggaugcgua      660

acaguaaugg cucaagauaa accaacuguu gauauagaau uaguaacaac aacaguaagc      720

aauauggcag aaguaagauc auacuguuau gaggcaagua uaagugauau ggcaucagau      780

agcagauguc ccacacaagg cgaagcauau cucgacaaac aaagcgacac gcaauaugua      840

uguaaacgaa cucuaguaga uagagguugg gguaacggcu guggacuauu uggaaaagga      900

aguuuaguaa ccugcgcuaa auucgcaugu ucaaaaaaaa ugacgggaaa aucaauacaa      960

ccagaaaacu uagaauauag aauaauguua agcguacaug gcagucaaca cucaggaaug     1020

auaguaaaug auacagguca ugagacugac gaaaaccgag ccaaaguaga aauaacucca     1080

aauucaccaa gagcugaagc aacacuugga gguuuuggca guuuagguuu agacugugaa     1140

ccaagaacag gacuagacuu uuccgaucua uauuaccuaa caaugaacaa uaaacauugg     1200

uuaguacaua aagaaugguu ucaugacaua ccauuaccuu ggcaugcagg agcagauacc     1260

ggaacaccuc acuggaauaa uaaagaagca cuaguugaau ucaaagaugc acaugcuaaa     1320

agacaaacug ucguuguacu agguagucaa gaaggagcag uucauaccgc acuagcggga     1380

gcacuugaag cagaaaugga cggggcaaaa ggaagauuaa gcucaggaca uuuaaaaugc     1440

agacuaaaaa uggauaaacu aagacuaaaa ggagucucau auaguuuaug uacagcagcg     1500

uuuacauuua caaagauucc agcagaaaca uuacauggaa caguaacugu agaaguacag     1560

uaugcaggaa cugacggacc auguaaaguu ccagcacaaa uggcuguuga uaugcaaacu     1620

uuaacuccag uaggaagauu gauuacagca aaucccguga uaacagaauc cacagaaaau     1680

ucuaaaauga uguuagaacu cgaucccccg uuuggagaua gcuauauagu aauaggagua     1740

ggagaaaaaa aaauaacaca ccauuggcac aggucagguu ccacaauagg uaaagcuuuu     1800

gaagcuacag uaagaggagc uaaaagaaug gcuguacuag gagauacagc augggauuuu     1860

ggaagcguag gcggagcacu uaauucauua ggaaaaggua uccaccaaau auucggagcc     1920

gcuuucaaau cuuuauuugg aggaaugagu ugguuuagcc aaauauuaau aggcacucua     1980

cuaauguggu uaggacuaaa cacuaaaaau ggaucuauau cauuaaugug uuuagcauua     2040

ggcggaguuc uaauuuuucu cucaacagca guaucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4884
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 1 opt

<400>  4884
gggagaaagc uuaccaugac aucaguagga auaguuggau uauuauuaac aacagcaaug       60

gcaguagaag uaacuagaag aggaaaugca uauuacaugu acuuagaucg aucagaugcc      120

ggugaagcga uuagcuuucc aacaaccaug ggaaugaaca aaugcuacau acaaauaaug      180

gaccuaggcc auaugugcga cgcuacaaug ucauacgaau guccuauguu agaugaggga      240

guagaaccug augauguuga cuguuggugu aauacaacaa guacuugggu aguauaugga      300

acaugccauc auaagaaagg agaagcaagg agaucaagac gagcaguaac acuuccaagc      360

cauucaacua gaaaacuuca aacaagaucu caaacguggc uagaaucaag agaauauaca      420

aaacacuuaa uaagaguaga aaauuggauu uuccgaaauc caggcuuugc auuagcagca      480

gcugcaauag cuugguugcu aggaaguuca acaucacaaa aaguaauuua ccucguaaug      540

auauuauuaa uagcacccgc auacucaauu cgaugcauag gaguaaguaa cagagacuuu      600

gucgaaggua ugucaggagg gacuugggua gauguaguac uagaacaugg agguugcgua      660

acaguaaugg cucaagauaa accaacagua gacauagaau uagucacuac aacgguauca      720

aacauggccg aaguacgauc auauuguuau gaagcaucaa uaucagacau ggccagugac      780

uccagauguc caacacaagg ugaagccuau cuagauaaac aaagugauac gcaauaugua      840

uguaaacgaa cacuuguaga cagagguugg ggaaacggcu gcgguuuauu ugguaaagga      900

aguuuaguaa caugugccaa auuugcaugu uccaagaaaa ugacagguaa aagcauacaa      960

cccgaaaauu uggaauaucg aauuauguua ucaguacacg gaucacaaca cucagguaug     1020

auagucaaug auacugguca cgaaacugau gaaaauagag caaaagucga aauuacaccc     1080

aauucgccua gagcugaagc aacguuagga ggauuuggcu cauuaggauu agauugcgaa     1140

ccaagaacag gucucgauuu cucagacuua uauuacuuaa ccaugaauaa uaaacauugg     1200

cucguucaca aagaaugguu ucaugauaua ccucuacccu ggcaugcagg agcagauacc     1260

gguacaccac auuggaauaa caaagaagcg cuaguagaau uuaaagacgc acaugcaaaa     1320

cgacaaacug uaguaguuuu aggaucacaa gaaggagcag uacauacugc auuagcugga     1380

gcauuagaag cagaaaugga uggagcaaaa ggaagguuaa guagugguca uuuaaaaugu     1440

agauuaaaaa uggauaaguu aagacuaaaa ggagugaguu auucacuaug cacagccgcc     1500

uuuacauuua cuaaaauucc agcagaaaca uugcauggaa cagucacagu ggaaguacaa     1560

uacgccggaa cagacggacc augcaaaguc ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaaccccag uaggaagacu aauaacagca aauccuguaa uaacugaauc aacggaaaau     1680

ucaaaaauga uguuggaauu agauccacca uuuggcgauu cauauauagu uauaggcguc     1740

ggagaaaaga aaauaaccca ucauuggcac agaucagguu caacaauagg aaaagcauuu     1800

gaggccacgg uaagaggcgc aaaaagaaug gcaguauuag gagacacagc augggauuuc     1860

ggaagcguug gaggagcacu caauagcuua gguaaaggaa uccaucaaau uuuuggagca     1920

gcguucaaau cauuauuugg uggaauguca ugguuuucac aaauauuaau aggaacuuua     1980

uuaguauggu uaggacuaaa uacuaaaaac ggaucuauaa gccuaaugug uuuagcacuc     2040

ggaggaguau uaauuuuccu aaguacagca guuagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4885
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 1 opt

<400>  4885
gggagaaagc uuaccaugac aucaguagga auaguugguc uauuauugac cacagcaaug       60

gcugccgaag ugacaagaag agguucagca uauuauaugu aucuugaucg gucugaugca      120

ggagaagcga uaaguuuucc aacaacucua ggaaugaaca aauguuauau acaaauaaug      180

gaccuagggc auauguguga cgcaacaaug aguuaugaau guccaaugcu agaugaagga      240

guagaaccag acgauguuga cuguuggugu aauaccacca guacaugggu aguauauggc      300

acaugucauc auaaaaaagg agaagcgcga cguaguagac gcgccguaac acucccauca      360

cauucaacaa gaaaacuuca aacaagaagc caaaccuggu uagaaaguag agaauacaca      420

aaacaccuua uaagaguaga aaacuggaua uuuagaaauc caggcuucgc auuagcagcu      480

gcagcaaucg caugguuacu cggaucauca acaucccaaa aaguaauaua ucuagugaug      540

auacuauuaa ucgcaccagc auacucaaua agauguauag gaguaaguaa cagagauuuu      600

guugaaggua ugaguggggg gacaugggua gauguaguac uagaacaugg cggaugcgua      660

acagucaugg cucaagauaa acccacagua gauauagaac ucguaacaac aacaguauca      720

aacauggcag aagucagauc auauuguuau gaggcaagua uaagugacau ggcaucagac      780

aguagguguc caacccaagg agaagcuuau cuagacaaac aaagugauac ccaauaugua      840

uguaaaagaa cuuuaguaga cagaggaugg ggaaauggau gcggauuauu uggaaaaggu      900

ucguuaguaa cgugcgcaaa auucgcaugu agcaaaaaaa ugacugguaa aucaauacaa      960

ccagaaaauc uagaauacag aauaaugcuc ucugugcacg guagucaaca uucuggaaug     1020

auaguaaaug auacaggaca ugaaacggau gagaacagag caaaaguaga aauaacacca     1080

aacaguccaa gagcagaagc aacacuaggg gguuucgguu cuuuaggauu agauugugaa     1140

ccaagaacag gacuagauuu uagcgaucua uauuauuuaa caaugaacaa caaacauugg     1200

uuaguacaua aagaaugguu ucaugauaua ccacucccau ggcaugcagg cgccgauaca     1260

ggcacaccac auuggaauaa caaagaagca cuaguggaau uuaaagaugc acacgccaaa     1320

agacaaacug uaguaguacu aggaucacaa gaaggagcug uacauacagc auuagcaggu     1380

gcacuagagg cagaaaugga uggagcaaaa ggaagauuau cuagcggcca ucuaaaaugc     1440

aggcuaaaaa uggauaaauu aagacuaaaa ggagugucau acucucucug uacugcagca     1500

uucacuuuua caaaaauacc cgcagaaacg uuacacggaa cuguaacagu cgaaguucaa     1560

uaugcaggaa cagauggacc auguaaagua ccagcacaaa uggcaguaga uaugcaaacu     1620

uuaacuccag uuggccgacu aauuacagca aauccaguua uuacagaguc aacagaaaau     1680

agcaaaauga ugcuagaauu agacccacca uucggagauu cauauauagu cauagggguc     1740

ggagaaaaaa aaauuacgca ucacuggcac agaagcggau caacaauagg aaaagcuuuc     1800

gaagcgacag uaagaggagc aaaaagaaug gcaguuuuag gggauacagc augggacuuu     1860

ggauccgucg guggagcacu aaauagucua ggaaaaggaa uacaucaaau auucggagcg     1920

gccuuuaaau cgcuauuugg aggcauguca ugguuuaguc aaauucuuau agguacuuua     1980

cuaauguggu uaggguuaaa uacgaaaaac gggucaauaa guuuaguaug uuuagcucua     2040

ggaggagugc ucauauuccu aucaacagcg guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4886
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 1 opt

<400>  4886
gggagaaagc uuaccaugac aaguguagga auuguaggau uauuacugac aacugcuaug       60

gcagcagagg uuacaagaag aggaagcgca uauuauaugu aucuagauag aucagaugcu      120

ggugaagcaa uuucuuuucc aacaacauua ggaaugaaua aauguuacau acaaauuaug      180

gauuuaggac auauguguga cgcuaccaug aguuaugaau guccaauguu agaugaagga      240

guugaaccag augacguaga uuguuggugu aauacaacau caacaugggu aguauauggg      300

acaugccauc auaagaaagg agaagcaaga cgaaguagac gagcuguaac auugccauca      360

cauucaacaa gaaaauugca aacuagauca caaacuuggc uagaaaguag agaauauaca      420

aaacauuuaa uccgaguaga gaauuggaua uuccgaaauc cuggauuugc auuagcagca      480

gcagcaauag cguggcuucu cgguaguuca acaucacaaa aaguuauaua cuuggucaug      540

auauuauuaa uagcuccggc auauagcauc agauguaucg gaguaaauaa ucgagauuuc      600

guagaaggaa ugucaggugg aacgugggua gauguaguau uagaacacgg uggaugugua      660

acuguaaugg cacaagauaa accaacuguu gauauagaac ucguaacaac gacaguaagu      720

aauauggcgg aaguacgaag uuauuguuau gaagcaucca uaucugauau ggcaucagac      780

agcagauguc caacccaagg agaagcauac cuggauaaac aaucagauac gcaauaugua      840

uguaaaagaa cuuuaguuga cagaggaugg ggaaauggau gcggcuuauu cggcaaagga      900

aguuuaguaa caugcgcaaa auuugcgugu aguaagaaaa ugacaggaaa aagcauacag      960

ccagaaaacc uagaauauag gauaauguug aguguacaug gcagucaaca uaguggaaug     1020

auagucaaug auacuggcca cgaaacagau gaaaauaggg caaaaguaga aaucacacca     1080

aauagcccuc gagcagaagc aacauuagga ggauuuggau cuuuaggucu agauugugaa     1140

ccaagaacag gcuuagauuu uucagacuua uacuaucuua caaugaauaa caaacauugg     1200

cuaguacaua aagaaugguu ucacgauaua ccacuaccuu ggcaugcagg agcagacacu     1260

ggaacaccac auuggaauaa caaagaagca cucguagaau uuaaagacgc acaugcaaaa     1320

agacagacag ugguaguuuu aggaucacaa gagggagcag uacauaccgc auuagcagga     1380

gcucucgaag cugaaaugga uggagcaaaa ggaagauuau caagcggaca uuuaaaaugc     1440

agacuaaaaa uggacaaauu aagauuaaaa gguguaaguu acagucuaug uacugcagca     1500

uuuacauuca cuaaaauacc agcggaaacc uuacauggaa cgguaacagu agaagugcag     1560

uacgcuggua cggauggacc cuguaaaguc ccugcacaaa uggccguaga uaugcaaacu     1620

cuaacaccug uaggacgacu aauuacagcu aauccaguaa uaaccgaaag cacagaaaac     1680

ucuaaaauga uguuagaauu agauccuccu uuuggagauu cauacauugu uauaggagua     1740

ggcgaaaaga aaaucacaca ccauuggcau agauccggaa guacaauagg aaaagcauuu     1800

gaggccacag uaagaggcgc aaaaagaaug gcaguacuag gagacacagc cugggauuuu     1860

gguucuguag gaggagcauu aaacaguuua ggcaaaggaa uacaucagau uuucggagca     1920

gcauuuaaau cauuauucgg gggaaugagu ugguuuucac aaauacuuau aggaacacua     1980

cuaauguggc uggguuuaaa uacuaaaaau ggaagcauau cccuaguuug ccuagcacua     2040

ggaggaguuc ugauauuucu aucaacagcu guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4887
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 1 opt

<400>  4887
gggagaaagc uuaccaugac gucaguagga auaguagggu uacuuuuaac gacagcaaug       60

gcaguagaag uaacacgaag aggauccgca uauuacaugu aucuugaccg aucagaugca      120

ggagaagcaa uaaguuuccc aacaacacua ggaaugaaua aaugcuauau acaaauaaug      180

gauuuagguc acaugugcga ugcaacaaug uccuaugaau guccgauguu agaugaaggu      240

guggaaccug augauguaga uuguuggugu aauacuacau cuacaugggu ugucuacggc      300

acaugucauc auaagaaagg agaagcgcga aggaguagga gagcaguaac acuaccguca      360

cacagcacaa gaaaauuaca aacuagauca caaaccuggu uggaauccag agaauauaca      420

aaacauuuaa ucagaguaga aaauuggaua uuuagaaauc caggguuugc uuuagcagca      480

gcagcaauag caugguuauu aggaaguuca acuagccaaa aaguuaucua ccuaguaaug      540

auucuauuaa uagcaccagc guauucaaua agauguauag ggguaaguaa uagagacuuc      600

guagaaggaa ugaguggagg aacuugggua gacgugguau uagaacaugg aggaugcgua      660

acagugaugg cacaagacaa accagcagua gacauagaau uaguaacaac aacaguaagc      720

aauauggcag aaguaagauc cuauuguuau gaagcaucaa uaucagauau ggcaagcgac      780

ucgagauguc cgacacaagg agaggcuuau cuagacaaac aaagcgauac ccaauaugua      840

ugcaaaagaa cauuaguaga uagaggaugg gguaaugguu gcggauuauu cggaaaagga      900

ucucuaguca caugugcuaa auucgcaugu ucaaagaaaa ugacaggcaa aaguauacaa      960

ccagaaaauu uagaauacag aauaauguua agcguucaug gaagucaaca uaguggaaug     1020

auaguaaacg auacgggaca cgaaacggau gaaaauagag cgaaagugga aauaacaccu     1080

aauucaccaa gagcagaagc uacacuaggu ggauuuggau cucuaggacu ggacugugaa     1140

cccaggacag gucuagauuu uagcgaccua uauuacuuaa cgaugaauaa uaaacauugg     1200

cuaguucaua aagaaugguu ucaugacaua ccauuaccau ggcaugcugg agccgacaca     1260

gguacaccac auuggaauaa uaaagaagca uuaguagagu ucaaagaugc ucaugccaaa     1320

aggcagacag uuguuguacu agggucacaa gaaggagcag uacauacagc ucuagcggga     1380

gcacuagaag cagaaaugga uggagcaaaa gggcgauuaa gcucuggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu aagauuaaaa ggaguaaguu auucauuaug cacagcggcc     1500

uuuacauuca caaaaauacc agcagaaaca cuacauggga cagugacagu agaaguucaa     1560

uaugcaggaa cagacggacc uugcaaaguu ccggcacaaa uggcaguuga uaugcaaaca     1620

cuaacaccag ugggaagauu aauaacagca aacccaguua uaacagaaag uacagaaaau     1680

agcaaaauga ugcuggaauu agauccacca uuuggugaca guuacauagu uauaggagug     1740

ggagaaaaga aaauaacaca ucauuggcau agaucuggga gcacaauugg aaaagcguuc     1800

gaagccacag uaaggggugc aaaaagaaug gcaguauuag gagauacugc augggauuuu     1860

ggaucaguag ggggugcuuu aaauucauua ggaaaaggaa uacaucaaau uuucggugca     1920

gcauuuaaaa guuuauuugg aggaauguca ugguucaguc aaauccucau uggaaccuua     1980

uuagucuggc ugggauuaaa uacuaaaaac gguucuauaa guuuaaccug ucuagcacua     2040

ggaggcguau uaauuuucuu aagcacagca guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4888
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 1 opt

<400>  4888
gggagaaagc uuaccaugac gucaguaggg auagucggau uauuauuaac aacugcuaug       60

gcaguugaag uuacaagaag aggaagugca uacuauaugu aucuugauag aucagaugcg      120

ggugaagcaa uaucauuucc uacuacacuu ggaaugaaca aauguuauau acaaauaaug      180

gaucuaggac auauguguga ugcaacaaug agcuacgaau guccgauguu agacgaagga      240

guagagccag acgauguaga uuguuggugu aacacaacau caacaugggu cguuuaugga      300

acgugucacc acaaaaaagg agaagcaaga agaucgcgaa gagcaguuac acuaccaagc      360

cauaguacca gaaaacuaca aaccagauca caaacauggc uagaaucacg ugaauauaca      420

aaacaucuaa uacgaguuga aaauuggaua uuuagaaauc cagguuuugc acuagcagca      480

gcaguaauag cauggcuacu aggaucguca acaagucaaa aagucauaua cuuagucaug      540

auccuacuaa uagcaccagc uuacuccaua agauguauag gaguaagcaa cagggauuuu      600

guugaaggua ugaguggcgg aacauggguu gauguuguac uagaacacgg ggguugugua      660

accguuaugg cacaagacaa accaacagua gacauagaac ugguaacaac aacaguauca      720

aauauggcag aaguucgaag uuauuguuau gaagcaagua uuagcgacau ggcuagugau      780

ucaagauguc caacacaagg ugaagcauau uuagauaaac aaucugauac acaauacguc      840

uguaaaagaa cacuugucga cagaggaugg ggaaauggau gugguuuauu uggaaaagga      900

agccucguaa cuugugcuaa auuugcaugu aguaaaaaaa ugacgggaaa aaguauacaa      960

ccagaaaauu uagaauauag aauaauguua agugugcacg gaucacagca uucagguaug     1020

auuguaaacg acacaggaca ugagacugac gaaaauagag caaaaguaga aauaacaccg     1080

aacagcccua gagcagaagc aacacuugga gguuuuggau cauuaggucu ugauugugaa     1140

ccuagaacag guuuagauuu cagcgacuua uauuaucuaa caaugaacaa uaaacauugg     1200

uuagugcaca aagaaugguu ucacgacaua ccgcugccau ggcaugcagg agcagacaca     1260

ggaacaccuc auuggaauaa uaaagaagcg cuaguugaau uuaaagaugc acacgcaaaa     1320

agacaaacag uaguuguacu aggaagccaa gaaggcgcag uucauacagc guuagcagga     1380

gcucuagagg cagagaugga uggcgcaaaa gggagauuaa guaguggaca uuugaaaugu     1440

agacuaaaaa uggauaaauu aagauuaaaa ggaguaucau auagccuaug cacagcagcc     1500

uuuacauuua caaaaauacc agcagaaaca uuacauggaa cuguaacugu ggaaguacaa     1560

uaugcaggaa cagauggccc auguaaagua ccagcacaaa uggcugucga caugcaaaca     1620

cuaacaccag uuggaagguu gauaacugca aauccaguga uuacagaauc aacagaaaau     1680

ucaaaaauga uguuagaguu agacccacca uuuggagaua gcuacauagu aauuggcgua     1740

ggagaaaaaa aaauuacgca ucauuggcac agaagcggau ccacaauagg aaaagcauuu     1800

gaagcaacag uaagaggagc aaaaaggaug gcaguguuag gagauacagc augggauuuu     1860

ggaagugucg gaggugcucu aaacucauua gguaaaggua uacaucaaau cuuuggggca     1920

gcauuuaagu cgcuauuugg aggaaugagc ugguuuagcc aaaucuuaau ugguacacua     1980

cuagucuggc uaggauuaaa cacaaaaaau gguucaauaa gcuuaaccug ucuagcauua     2040

gggggcguac uaaucuucuu aucaacagca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4889
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 1 opt

<400>  4889
gggagaaagc uuaccaugac aucagugggu aucguaggau uauuacuuac aacagcaaug       60

gcaguugaag uaacaaggag gggauccgca uauuauaugu acuuagacag aagugacgcc      120

ggugaagcaa uaucauuucc aacaacacua ggaguuaaua aauguuauau acaaauuaug      180

gaccuaggac acauguguga ugcaacaaug uccuacgaau guccaaugcu agacgaagga      240

guggaaccag augauguaga uuguuggugu aauacaacaa guacaugggu aguuuauggg      300

acaugucauc auaagaaagg agaagcacga agaucaagga gagcugucac auuaccauca      360

cacaguacga gaaaacuaca aacaagaagu caaacauggc uagaaucaag ggaauauacu      420

aaacaucuua uaaggguuga aaauuggaua uucagaaauc caggauucgc acuagcagcu      480

gcagcuauag cguggcuuuu ggguaguagu acuagccaaa aagucauaua uuugguuaug      540

auucuacuaa uugcaccggc auauuccaua cguuguauag gaguaucaaa uagagacuuu      600

guugaaggaa ugucaggagg aaccugggua gauguaguac uagaacacgg aggcuguguu      660

acggucaugg cacaagauaa accaacagua gauauagaac ugguuacaac aacaguaagc      720

aauauggcug aaguacgaag cuacugcuac gaagcaagua uaucagauau ggcaucagac      780

ucacgauguc caacacaagg agaagcauau uuagauaaac aaagugauac ucaauaugua      840

uguaaaagaa cucuaguaga uagaggaugg gggaaugggu gcgguuuauu cggaaaaggu      900

agucuaguua ccugugccaa auuugcaugc ucaaagaaaa ugacuggaaa aaguauacag      960

ccugaaaauu uagaauauag aauaaugcua ucaguacaug gaagucaaca uuccggaaug     1020

auagucaaug auacaggaca ugaaacagau gaaaauagag caaaaguaga aauuacacca     1080

aacaguccaa gagcagaagc aacauuagga gguuuugguu cauuaggauu agauugcgaa     1140

cccagaacag gacuagacuu uagugacuua uauuaccuaa caaugaauaa uaaacauugg     1200

uuagugcaua aagaaugguu ucaugauaua ccauuaccau ggcacgcagg agcugauacg     1260

ggaacaccac acuggaauaa uaaagaggcu cugguagaau uuaaagaugc acacgcaaaa     1320

agacaaacag uaguuguuuu ggguagccaa gaaggugcag uucacacagc guuggccgga     1380

gcauuagagg ccgaaaugga uggugcaaaa ggaagacuau caaguggaca ucuaaaaugu     1440

agacuaaaaa uggacaaauu aagauuaaaa ggaguaaguu auagccuaug cacagcugca     1500

uuuacauuca caaaaauacc agccgaaaca uuacauggaa caguaacagu agaaguucaa     1560

uaugcaggaa cagacggacc augcaaagua ccugcacaaa uggcugucga uaugcaaaca     1620

uuaacuccag uuggaagauu gauaacagca aauccaguaa uaacagaaag uaccgaaaac     1680

agcaaaauga uguuagaacu ugauccaccu uuuggugaua guuauauagu gauuggcgua     1740

ggagauaaga aaauuacaca ccauugguau agaucaggau caacaauagg aaaagcauuc     1800

gaagcaacug uaagaggagc aaaacgaaug gcaguauuag gagacacugc augggauuuu     1860

ggaagcguag gaggagcguu aaauucacua ggcaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaaa guuuguuugg cggaaugagc ugguuuaguc aaauauuaau aggaacauua     1980

uuaguauggu uaggacuaaa cacgaaaaau ggaucaauaa gucuaacaug uuuagcucua     2040

gguggaguau uaauauuuuu aaguacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4890
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 1 opt

<400>  4890
gggagaaagc uuaccaugac aucaguugga auaguuggac uauuacucac uacagcaaug       60

gcaguagagg uaacacgaag aggcucagca uauuauaugu accuugauag gucagaugcc      120

ggaaaagcaa uuucauuucc aacaacauua gguguaaaca aaugcuauau acaaauaaug      180

gaucuaggcc auauguguga ugcaacuaug ucauaugaau gcccaauguu agaugaagga      240

guagaaccag augauguaga cuguuggugu aauacuacaa guacuugggu aguauaugga      300

acaugucacc auaaaaaagg agaagcaaga agaagcagga gagcagucac auugcccuca      360

cauucuacaa gaaaauugca aacgagaagc caaacuuggu uggaaucaag agaauacaca      420

aaacacuuaa uaagaguaga aaauuggaua uuuagaaacc caggauucgc auuggccgca      480

gcagcuauag caugguuauu aggaucuucu acgucgcaaa agguaauaua ucuaguaaug      540

auauuauuaa uagcccccgc guauucaauu agauguauug gaguaucaaa uagagacuuc      600

guagaaggga ugucaggagg aacauggguc gauguuguuu uagaacacgg uggaugcguc      660

accguuaugg cacaagauaa accuacaguc gauauugaac uaguaacuac uacugucuca      720

aacauggcag aaguucgaag uuauuguuau gaagcaucaa uuucagauau ggcaucagau      780

aguagauguc caacccaagg agaagcuuau uuagacaaac aaagcgacac acaauauguu      840

uguaaaagaa cauuaguuga uaggggcugg ggaaaugguu guggauuauu cggaaaagga      900

aguuuaguua caugugcaaa auucgcuugu ucuaaaaaaa ugacgggaaa aaguauccaa      960

ccugagaauu uagaauauag aauaaugcua ucaguacacg gaagucaaca cagcgggaug     1020

auaguaaaug acauaggaca ugaaacggau gaaaauaggg cuaaaguaga aauaacaccg     1080

aacucaccaa gagccgaagc aacacuuggu gguuucggau cauuaggacu ggauugcgaa     1140

ccaagaacug gauuagacuu cagcgaucua uauuacuuaa caaugaauaa uaaacacugg     1200

uuaguacaua aagaaugguu ccaugauaua ccauuacccu ggcaugcagg agcagauacg     1260

ggaacaccac auuggaauaa caaagaagcc cuaguagaau ucaaagaugc acaugccaaa     1320

agacaaacag uaguaguucu agggagucaa gaaggagcag uacauacugc acuagcagga     1380

gcauuggaag cagagaugga uggagcaaaa ggaagauuau caagugguca uuuaaaaugc     1440

agauugaaaa uggauaaauu acgauuaaaa ggaguaucau acucucuuug cacagcagca     1500

uuuacuuuca cuaaaauucc agcugaaacu cuucauggaa caguaacagu agaaguccag     1560

uacgcgggaa cagauggacc augcaaagua ccugcacaaa uggcaguaga caugcaaaca     1620

uuaaccccag uaggaagauu aauaacagca aacccuguaa uaacagaaag uacugaaaac     1680

ucuaaaauga uguuagaauu agauccaccu uuuggagauu cauauauagu cauaggaguu     1740

ggagauaaaa aaauaacaca ccacuggcau aggucaggaa gcacaauugg aaaagcauuu     1800

gaagcuaccg uaagaggcgc aaaaagaaug gcaguauuag gagacacagc augggauuuc     1860

gguucuguag gaggugcguu aaacucauug gguaaaggga uacaccaaau cuuuggagcc     1920

gccuuuaaga gccuauuugg aggaaugagu ugguuuaguc aaauacuaau aggaacacua     1980

uuaguauggu ugggacuaaa uacaaaaaau ggaagcauau cacuuacaug uuuagcauua     2040

ggagguguau uaauauuucu gucaacugca guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4891
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 1 opt

<400>  4891
gggagaaagc uuaccaugac aaguauagga auuguagguc uacuucucac uacagcaaug       60

gcagcugaga uaacaagacg ggguagugca uacuauaugu accuagacag aagugacgca      120

gguaaagcaa uuucauuugc aacuacauua ggcguaaaca aaugucaugu acaaauaaug      180

gauuuaggac auaugugcga cgcaacaaug ucauaugaau gcccuaugcu ggaugaggga      240

guugaaccug acgauguaga uuguuggugu aauacaacau caacaugggu agucuacgga      300

acaugccauc auaagaaagg agaagcaaga agaaguagga gagcagugac acuaccuucu      360

cauaguacaa gaaaauuaca aacuagaagu caaacauggc uagaaucaag agaauauaca      420

aaacauuuaa uaaaaguuga aaauuggaua uuuagaaauc caggauuuac auuaguagcu      480

guugcaauag cauggcuauu gggaucaagu accagucaaa aaguuauuua ucuaguaaug      540

aucuuauuaa uagccccugc auauagcaua cgauguauag gaguuucaaa cagagauuuu      600

guagaaggaa ugaguggagg uaccugggua gauguaguau uagaacaugg aggcugcgua      660

acggugaugg cucaagauaa gccaacaguu gauauagaau uaguaacaac aacaguaagc      720

aacauggcag aaguuagaag cuacuguuac gaagcaagua uaagugauau ggcaucagau      780

aguagaugcc caacgcaagg agaagccuac uuagauaaac aaagugauac acaauaugua      840

ugcaaaagaa cauuaguuga ucgaggaugg ggaaacgguu gcgguuuauu uggaaaagga      900

ucuuuaguaa cuugugcaaa guuuacaugu aguaagaaaa ugacugguaa aagcauacaa      960

ccagaaaauc uagaauauag aauaauguua ucaguacaug gaagccaaca cagcggaaug     1020

auaguuaaug acaccggaca cgaaacagac gaaaauagag ccaaaguaga aguuacacca     1080

aacaguccuc gagcggaagc aacacuaggc ggauucggaa guuuaggauu agacugugaa     1140

ccuagaacag gccuagauuu uagugacuua uacuacuuaa caaugaauaa caaacauugg     1200

uuaguacaca aagaaugguu ccaugacauc ccacuaccau ggcacgcugg ugcagauacu     1260

ggaacaccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc acaugcuaaa     1320

agacaaacag uuguuguuuu aggaucucaa gaaggagcag uacauacagc acuagcuggc     1380

gcucuggagg cugaaaugga uggagcgaaa ggaagauuau ucucaggaca cuuaaaaugu     1440

agauuaaaaa uggauaaauu gagacuaaag ggaguaaguu auagucuaug uacagcagca     1500

uuuacguuua caaaagugcc ugcagaaaca cuacauggaa cugucacggu agaaguucaa     1560

uaugcaggaa cugauggacc guguaaagua ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaaccccug uaggaagacu gauaacagca aacccgguaa uaacagaaag uacagaaaac     1680

aguaaaauga uguuagaauu ggacccgcca uucggagaua gcuauauagu aauuggcgua     1740

ggagauaaga aaauaacuca ccauuggcac agaaguggau caacaauagg aaaagcauuc     1800

gaagcaacag uaagaggagc aaaacgaaug gcaguauugg gagauacagc augggauuuu     1860

ggaucaguag gaggcguauu uaacucgcua ggaaaaggga uacaucaaau cuuuggagca     1920

gccuucaaaa guuuauuugg aggaaugagc ugguuuaguc aaauacuuau aggaacacua     1980

uuaguauggu uaggauuaaa cacuaaaaac ggaaguaucu cuuuaacaug uuuagcauua     2040

ggaggaguga ugauauucuu aucgacagcu gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4892
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 1 opt

<400>  4892
gggagaaagc uuaccaugac aaguauagga auaauagguc ucuuguuaac gacagcuaug       60

gcagcagaaa uaacaagacg agggagugca uauuauaugu accuagaccg uucugacgcc      120

ggaaaagcua uaucauuugc cacaaaccuc ggaguaaaca agugucaugu acaaauaaug      180

gaucucgguc auauguguga cgcgacuaug aguuaugaau guccaaugcu agaugaagga      240

guagaaccag acgacguuga uugcuggugu aauacaacaa gcacuugggu aguauaugga      300

acaugucauc acaagaaagg ggaggccaga agaucaagac gagcaguaac auuaccaucc      360

cacagcacaa gaaaauuaca aacaagaagu caaacauggu uagaaaguag agaauauacu      420

aaacauuuaa uaaaaguuga aaauuggauu uucagaaauc caggauucgc auuagcagca      480

guagcaauag ccuggcuauu aggaucauca acuucacaaa aaguaauuua ucuggucaug      540

auauuacuaa uagcaccagc auauagcaua agaugcauag gaguaaguaa cagagauuuu      600

guagaaggaa ugaguggagg aacauggguu gaugucguau uagaacaugg agguugugua      660

acuguuaugg cgcaagauaa accaacagua gacaucgaau uaguuacaac aacaguaagc      720

aauauggcug aaguaaggag uuauuguuau gaagcaucca uuucggauau ggccucagau      780

aguagaugcc cuacacaagg agaagcauau uuagauaaac aaucagacac acaauauguc      840

ugcaaacgaa cucuagucga uagaggaugg gguaauggcu guggccuauu cgguaaagga      900

ucccuaguaa caugcgcaaa auucacaugc ucuaagaaaa ugacagggaa aucaauccaa      960

ccugaaaauu uggaauaucg aauaauguua aguguacacg ggagucaaca cucaggaaug     1020

auaguaaaug auauagggca ugaaacagau gaaaacagag caaaaguaga aguaacgccu     1080

aauucaccaa gagcugaagc aacacuaggu ggauuuggga guuuaggauu agacugugaa     1140

ccaagaacag gauuagauuu cucagaccuc uacuacuuaa caaugaauaa uaaacacugg     1200

cuaguacaua aagaaugguu ccaugauauu ccuuuaccau ggcacgcagg agcagauaca     1260

ggcacaccuc auuggaacaa uaaagaagcu cuaguagaau uuaaagaugc acaugcaaaa     1320

agacaaacug ugguuguauu agguucacaa gaaggagcag uacauacagc acuagcuggc     1380

gcauuggaag cagaaaugga cggagcaaaa gguaggcucu uuaguggaca ucuaaaaugu     1440

agguuaaaaa uggacaaacu uagguuaaaa ggaguaucau acucacuaug cacugcugcu     1500

uucacauuca caaaaguacc cgcagaaaca uuacacggaa cagugacagu ugaaguacaa     1560

uaugcuggaa cugauggucc cuguaaggua ccagcacaaa uggccguaga uaugcaaaca     1620

uuaacuccag uagguagacu aauaaccgca aauccaguaa uuacagaauc cacagaaaau     1680

aguaagauga ugcuagagcu agauccacca uuuggagaua guuauauagu cauaggagua     1740

ggugacaaga aaaucacaca ucacuggcau agaaguggca gcacaauugg aaaagcauuu     1800

gaagcaacag uaagaggagc aaaaagaaug gcaguauuag gugauacagc augggauuuu     1860

ggaaguguag guggaguuuu uaauucacua ggaaaaggag uacaucaaau auuuggagcc     1920

gcuuucaaau cacuauuugg aggaaugagu ugguuuucuc aaauauuaau aggaacauua     1980

uuaguauggu uaggucuaaa cacuaaaaau ggaucaaucu cauuaacaug uuuagcacuc     2040

ggaggaguua ugauuuuccu aagcacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4893
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 1 opt

<400>  4893
gggagaaagc uuaccaugac uucaauaggu auaauagguc uauuacuaac aacagcaaug       60

gcugcagaaa uaacacgaag aggaucagcu uacuacaugu aucuagacag aagugaugcu      120

ggaaaagcaa uuagcuucgc aacaacacuc ggaguuaaua aaugccacgu ucaaauaaug      180

gacuuaggac auauguguga ugcaacuaug aguuacgaau guccaaugcu ugacgaaggu      240

guugaaccag augauguaga cuguuggugu aauacgacua gcacgugggu aguauacgga      300

acaugucauc auaaaaaagg cgaagcuaga agaagucgac gagcaguuac acuaccgagu      360

cacaguacaa gaaaacuaca aacacgauca caaacauggu uagaaaguag agaauauaca      420

aaacaccuaa uaaagguuga gaauuggauc uucagaaauc cgggguucgc ucuuguagca      480

guagccauag caugguuauu aggaucuagc acaucacaaa aaguaaucua ucuaguuaug      540

auacuuuuga uagcaccagc uuauaguaua agauguauug gaguaucaaa uagagacuuc      600

guagaaggaa ugucuggagg aaccugggua gauguaguau uagaacacgg cggaugcguu      660

acaguaaugg cccaagauaa accaacagua gacauagaau uaguaaccac gacaguaagu      720

aauauggcgg aagugagaag uuauugcuau gaagcaagua uaucagauau ggcaucagau      780

ucacgaugcc caacacaagg agaagccuau uuagauaaac aaagcgauac acaauacguu      840

ugcaaaagaa cucuaguaga cagaggaugg ggaaauggau guggauuauu cggaaaagga      900

ucuuuaguaa ccugugcaaa auuuacaugc aguaaaaaaa ugacagguaa aaguauacaa      960

ccagaaaauu uagaauauag aaucaugcuu ucaguacacg gaucacaaca uagcgguaug     1020

auaguaaacg acauaggaca ugaaaccgau gaaaauaggg caaaaguaga gguaacacca     1080

aacucaccaa gagcagaagc uacuuuagga ggauuuggau cauuaggguu ggauugugaa     1140

ccacgaaccg gacuagauuu uagugaucua uacuaucuaa caaugaauaa caaacauugg     1200

uugguacaua aagaaugguu ucacgacauu ccucuaccau ggcaugcugg ugcagauacu     1260

ggaacaccac auuggaauaa uaaagaagca cuaguagagu uuaaagaugc ccaugccaaa     1320

cgacaaacag uaguaguacu aggaagucaa gaaggagcag uacauacagc auuagcugga     1380

gcauuagaag cggaaaugga uggagcaaaa ggaagacuau ucagugguca ccuaaaaugu     1440

agauuaaaaa uggauaaauu aagauuaaaa ggagugucau auucccuaug cacagcggca     1500

uucacauuua cgaaaguucc agcagaaaca cuccauggaa cagugacagu agagguacaa     1560

uacgcuggaa cugauggucc augcaaagua ccggcacaaa uggcgguuga uaugcagaca     1620

uuaacaccag uagggagguu aauaacagca aauccaguaa uuacagaaag cacagaaaac     1680

ucuaaaauga ugcuagaguu agauccaccc uuuggagaca guuacaucgu aauaggagua     1740

ggagauaaaa aaauaacgca ucauuggcau agaucagguu cuacuauugg caaagcuuuu     1800

gaggcaacag uaagaggagc aaaaaggaug gcagucuuag gugauacggc augggauuuu     1860

ggcucuguag gaggaguuuu uaacaguuua gguaaaggaa uucaccaaau auuuggagcg     1920

gcauuuaaaa gcuuauuugg aggaaugagu ugguuuucac aaauacuuau agguacguua     1980

uuagucuggc uuggauuaaa cacaaaaaau ggaaguauau cacuaacuug cuuagcauua     2040

ggaggcguaa ugauauuuuu aaguacugca guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4894
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 1 opt

<400>  4894
gggagaaagc uuaccaugac aagcauugga auuauaggac uauuauuaac aaccgcaaug       60

gccgcagaaa uaacgagacg uggaagcgca uacuauaugu auuuagaccg aucugaugca      120

ggaaaagcua uuucuuuugc gacaaauuua ggaguuaaua aaugccaugu ucaaauaaug      180

gaucuaggac auauguguga ugcaacaaug ucauaugaau guccaauguu agaugaagga      240

guagaaccag acgacguaga uuguuggugc aacacaacau caacaugggu aguauacgga      300

acgugucacc auaagaaagg ugaagcaaga agaucuagga gagcaguaac auuaccaucg      360

cauagcacaa gaaaacuaca aacaagaagu caaaccuggc uagaaucaag agaauacaca      420

aaacaccuca uaaaagugga aaauuggaua uucagaaacc caggauucgc uuuagcagca      480

guagcaauag cauggcuuuu aggaagcagu acaucccaaa aaguaauaua uuuaguuaug      540

auauuauuaa uagcuccagc auacucaauc aggugcauag gaguaaguaa uagggauuuc      600

guugaaggaa ugucaggagg aacaugggua gacguaguau uagagcacgg agguugcgua      660

acgguaaugg cacaagacaa accaacagug gauauagaau uaguaacaac aacuguuagu      720

aauauggcgg aaguacgauc auauugcuau gaggccucua uaucagacau ggcaucagac      780

agcagaugcc caacccaagg agaggcuuac uuagauaaac aaagugacac acaauacgua      840

uguaaaagaa cacucguuga ucgaggcugg ggaaauggau gcggauuauu uggaaaagga      900

ucuuuaguua caugcgcaaa auuuacaugu aguaagaaaa ugacagggaa aagcauacaa      960

ccagaaaauu uagaauauag aauaaugcua ucuguacaug gaucacaaca uucagggaug     1020

auaguaaacg augaaaaccg agcaaaaguc gaaguaacuc caaauucacc ucgagcggaa     1080

gcaacguuag gugguuucgg uaguuuaggu uuagauugcg aaccuagaac aggacuggau     1140

uucagcgauu uauauuaccu aacuaugaac aauaaacauu gguuagugca uaaagaaugg     1200

uuucacgaua uaccacuccc auggcacgca ggagcugaua caggaacacc acauuggaau     1260

aauaaagaag cucuaguaga auuuaaagau gcacacgcaa aaagacaaac aguaguggua     1320

cuaggaaguc aagaaggagc cguacauaca gccuuagcag gagcauuaga agcugaaaug     1380

gauggugcaa aaggaagguu auuuucagga cauuuaaaau guagacucaa aauggauaaa     1440

uuaagauuaa aagguguaag uuauucacua ugcacagcag cuuuuacauu uacaaaagua     1500

ccagcagaaa cacuacacgg gacagugaca guagaaguac aguacgcagg aacagauggg     1560

ccauguaaag uaccagcaca aauggcugua gacaugcaga cauugacacc gguuggaaga     1620

uuaauaacag caaauccagu aauaacugaa aguacugaaa auucaaaaau gaugcuagaa     1680

cuagacccac cauuuggcga cuccuauaua guuauaggag ugggugauaa gaaaaucaca     1740

caucauuggc auagaucagg aaguacgaua ggaaaagcgu uugaagccac gguuagagga     1800

gcuaaaagaa uggcagugcu aggagauacg gcuugggauu uuggaagugu aggaggcgug     1860

uuuaauagcc uagggaaagg aauacaucaa auauuugggg cagcuuucaa aagucuauuu     1920

ggagguauga guugguuuag ucaaauauua auagguacac uauuaguaug guuaggauug     1980

aacacuaaaa augggagcau uaguuugacu uguuuagcac uagguggugu aaugauauuu     2040

uuaucaacag caguaagugc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4895
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 1 opt

<400>  4895
gggagaaagc uuaccaugac cucaauuggu auugucggac uacuccuaac aacagcaaug       60

gcagcagaaa ucacucgaag gggaucggcg uacuauaugu aucuugauag gagcgacgca      120

ggaaaagcaa ucucguuugc uacaacauua ggaguaaaca aaugccaugu acaaauaaug      180

gauuugggac auaugugcga ugcaacaaug uccuaugagu guccuauguu ggacgaagga      240

guugaacccg acgacguaga cuguuggugu aauacgacaa gcacaugggu uguauauggu      300

acaugucauc auaaaaaagg ugaagcuaga agaucucggc gagcagugac auuaccauca      360

cauucaacaa ggaaacuaca aacuagauca caaacauggu uagaaucacg agaauauacg      420

aaacaucuaa ucaaaguaga aaacuggauu uuucguaacc caggauuugc ccuuguagca      480

guagcaauag cauggcuucu aggaucgagu acaucccaaa aaguaauuua uuuaguaaug      540

auacuauuaa uagcaccagc auauagcaua agauguauag gaguuaguaa cagagacuuc      600

gucgaaggaa ugucaggcgg aacauggguu gauguagucc uagaacacgg cggaugugua      660

acaguaaugg cucaagauaa accaacagua gauauagaac ucgucacgac aacaguauca      720

aauauggccg aaguaagaag uuauuguuau gaagcgagua uuagugacau ggccucagau      780

ucaagauguc caacccaggg cgaagcauau cuugauaaac aaucugacac acaauaugua      840

ugcaaaagaa cacuuguaga cagaggaugg ggaaauggau gcggacuauu uggaaaagga      900

agccuaguaa caugugcaaa auucacuugu ucaaaaaaga ugacaggaaa aagcauucaa      960

ccugaaaacu uagaauaucg aauaauguua ucuguucaug gaucacaaca uucuggaaug     1020

auaguaaaug acacuggaca ugaaacugac gaaaacagag caaaaguaga agucacaccc     1080

aauucaccaa gagcagaagc uacacuagga ggauuuggua gucuaggucu agauugugaa     1140

ccaagaacug gacuagauuu cucagacuua uacuaucuga caaugaauaa caaacacugg     1200

uuaguacaua aggaaugguu ucaugacaua ccucucccuu ggcacgcagg agcagauaca     1260

gguaccccac auuggaacaa uaaagaagca cuaguagaau uuaaagacgc ccaugcaaaa     1320

agacaaacag uaguagucuu aggaagccaa gaaggagcag uacauacagc auuagcagga     1380

gcucuagaag ccgaaaugga cggagcaaaa gguagacuau uuucugguca ucuaaaaugc     1440

cgacuaaaaa uggacaaauu aagacuaaaa gguguaucau acagcuuaug cacagccgca     1500

uuuacauuca cuaaaguccc agcagaaaca uuacacggga caguaacagu agaaguacaa     1560

uaugcaggaa cagacggacc augcaaaguc ccagcacaaa uggcaguaga caugcaaaca     1620

uuaacuccag uaggaagacu aauaacagcu aacccaguua uuacagaauc gacagaaaau     1680

ucaaaaauga ugcuagaacu ugauccacca uuuggugauu cauauauagu uauaggagua     1740

ggagauaaaa aaauaacaca ucauuggcau agaucaggau caacaauagg aaaagcauuu     1800

gaagcaacag uaaggggcgc caaaagaaug gcaguauuag gagacacagc augggauuuu     1860

gguucuguug gagguguauu caauucauua ggaaaaggaa uccaccaaau auuuggagcc     1920

gcauuuaaau cucuauuugg aggaauguca ugguucucac aaauauuaau aggaacauug     1980

uuaguauggc uaggauuaaa cacgaaaaau gggucaauua gucuaaccug ucuagcauug     2040

ggaggaguaa ugauauuucu aucaacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4896
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 1 opt

<400>  4896
gggagaaagc uuaccaugac aucuaucggu auaguaggau uacuauuaac aacggcaaug       60

gccgcagaga uaacucgaag aggaucagcg uacuauaugu acuuagauag gucugacgcc      120

ggaaaagcaa uaaguuuugc uacuacauua ggaguaaaua aaugucacgu acaaauaaug      180

gaucuaggac auauguguga cgcuacuaug ucauaugaau gcccaauguu agaugaaggc      240

guugaaccgg augauguaga uuguuggugc aauacuacau caacaugggu aguauaugga      300

acaugucaac auaagaaagg ugaagcccga agaagucgga gagcugucac auuaccauca      360

cacucaacaa gaaaauuaca aacaagauca caaacauggu uagaauccag agaauacaca      420

aaacaucuaa uaaaaguuga gaacuggauu uuuagaaacc caggcuuugc uuuaguagca      480

guggcaauag cgugguuauu gggaaguagu acaagucaaa aagugauaua uuuaguaaug      540

auucuauuaa uagcuccagc auauaguauc agauguauag guguaucaaa uagagauuuu      600

guagaaggaa ugagcggagg aaccugggua gaugucguau uagaacaugg aggaugugua      660

acuguuaugg cacaagacaa accuaccgua gauauagaau uaguaacaac uacuguuagu      720

aauauggcug aagucagaag cuauugcuac gaagcaagua uaagugauau ggcaagugau      780

ucgagauguc caacucaagg cgaagcauau cuugauaaac aaagcgauac acaauaugua      840

uguaaaagga cguugguaga uagagguugg ggaaacggau guggcuuauu cggaaaagga      900

agucuaguua cuugcgcgaa auuuacaugu agcaagaaaa ugacagguaa aaguauacaa      960

cccgaaaauc uagaauauag aaucauguua ucaguacacg gaucacaaca uaguggaaug     1020

auaguaaaug auauaggaca cgaaaccgau gaaaauagag ccaaaguuga aguaacgcca     1080

aauucgccaa gagcugaagc aacauuagga ggauucggau cucuuggauu agauugcgag     1140

ccaagaacag gauuagacuu uagugauuua uacuaucuaa cuaugaauaa caaacauugg     1200

uuaguacaua aagaaugguu ucaugauauc ccauuaccuu ggcaugcagg agcagauaca     1260

gggacaccuc auuggaauaa uaaagaagca uuagucgagu ucaaagacgc ucaugcaaaa     1320

agacaaaccg uaguaguacu aggaucacaa gaaggagccg uacauacggc gcuugcagga     1380

gcauuagaag cagaaaugga uggagcaaaa ggaagauuau uuucuggaca uuuaaaaugu     1440

agauuaaaaa uggauaaacu gagauuaaaa ggaguuucuu auucuuuaug uacagcaguu     1500

uguacagcag cuaaaguccc agcagaaaca cuacauggaa caguaacagu agaaguacaa     1560

uaugcaggaa ccgauggucc auguaaagua ccagcucaaa uggcagucga uaugcaaaca     1620

uuaacuccgg uaggaagacu aauuacagcu aacccaguaa uaacagaguc aaccgaaaau     1680

agcaaaauga uguuagaauu agauccacca uuuggagaua gcuacauugu aauaggaguu     1740

ggagauaaga aaauaacaca ucauuggcau agaucgggaa gcaccauugg gaaagcauuc     1800

gaagcgacag ugaggggagc aaaaagaaug gcaguauuag gagauacagc gugggauuuu     1860

ggaucuguag gaggaguauu uaauaguuug ggaaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaau cauuauuugg aggaaugagc ugguuuucac aaauauuaau aggcacacua     1980

uuggucuggc ucggacugaa uacuaaaaac ggaaguauua guuuaacgug ccuagcauua     2040

ggaggcguaa ugauauuccu uaguacagca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4897
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 1 opt

<400>  4897
gggagaaagc uuaccaugac cucaauagga auaguaggcu uguuguuaac aacagcaaug       60

gcagccgaaa uuacaagaag agguuccgca uauuauaugu accuagauag aucagaugca      120

gggaaagcaa uaaguuuugc aacaacuuua ggagucaaua aaugucaugu acaaauaaug      180

gauuugggac auauguguga ugcaacaaug agcuacgaau guccaauguu agaugaagga      240

guugaaccug acgauguaga uuguuggugu aauacaacaa guacaugggu aguauacgga      300

accugucauc auaaaaaagg agaagcucga agaagcagaa gggcuguaac acuaccgucu      360

cauagcacua gaaaauuaca aacacgaucc caaacauggc uagaaaguag agaauacaca      420

aaacauuuaa uaaaaguaga aaauuggaua uuuagaaauc ccggauucgc uuuaguagcc      480

guagcaauug caugguuauu aggaucaucu acaucacaaa aaguaauaua ucuuguaaug      540

auacuauuaa uagcgccggc auauaguauu agaugcauag gaguaaguaa uagagauuuu      600

guagaaggaa ugaguggugg uacgugggua gaugucguuc uagaacacgg aggaugcgua      660

acaguaaugg cacaagacaa accgaccgua gacauagaau uaguuacaac uaccguuuca      720

aacauggccg aagugagauc uuacuguuau gaagcuucaa uaagugauau ggcaucagau      780

ucaagaugcc cuacacaagg agaagcguac cuugacaaac aaucggauac acaauaugua      840

ugcaaaagaa cacuaguaga ucgaggaugg ggaaacgguu guggacuguu uggaaaagga      900

aguuugguaa cuugugcaaa auuuacaugu ucaaaaaaaa ugacagguaa gucaauacaa      960

ccagaaaacc uugaguauag aauaaugcua ucaguacaug gaagucagca cucaggaaug     1020

auaguaaaug acgaaaacag agccaaagua gaaguuacuc ccaauagucc acgagcggaa     1080

gcaacauugg gaggguuugg aucucuaggu cuagauugcg aaccgagaac cggauuagau     1140

uucagugauu uauauuauuu aacaaugaau aauaaacacu gguuaguuca caaagaaugg     1200

uuccacgaua ucccauuacc auggcaugcu ggagcggaua cgggcacacc acauuggaau     1260

aacaaagaag cacuaguuga auuuaaagau gcacaugcaa aacgucaaac aguaguagua     1320

uuaggauccc aagaaggcgc uguacauaca gcacuagcag gugcauuaga agcugaaaug     1380

gauggugcua aaggacgcuu auuuucagga caucuaaaau gucgucuaaa aauggacaaa     1440

uuaagacuua aaggaguaag uuauagcuua uguacugcag cauuuacuuu uacaaaagua     1500

ccagcugaaa cauuacacgg aacagugaca guugaaguac aauaugcugg gacagacgga     1560

ccauguaaag uaccagccca aauggcagua gauaugcaaa cauuaacgcc uguaggucgu     1620

uuaauaacug cuaacccugu cauaacggaa ucaacagaaa auucaaaaau gauguuagaa     1680

uuagauccac cuuucggaga cucauacaua guaauaggag uuggagacaa gaaaauaacu     1740

caccacuggc auagaucagg aaguacgauu gguaaagcau uugaagcaac ggugagagga     1800

gcaaaaagaa uggcaguauu aggggauaca gcgugggauu uuggaagugu aggaggaguu     1860

uuuaacucau uaggaaaagg uauacaccaa auauuuggug cagcauuuaa aucucuauuu     1920

ggagguaugu caugguuuuc acaaauauua auagguacac uauuaguaug guugggacua     1980

aauacaaaaa auggaucaau aucucuaaca ugcuuagcuu uaggaggagu aaugauauuu     2040

uuaaguacag cagugucagc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  4898
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 1 opt

<400>  4898
gggagaaagc uuaccaugac uucaauagga auaaucgggu uacuauuaac aaccgcaaug       60

gcagcugaaa uaacccgaag aggaucagca uacuauaugu accuagauag aucagacgca      120

ggaaaagcaa uaagcuuugc aacgacgcuc ggaguaaaca aaugucaugu ccaaauaaug      180

gauuugggac acauguguga cgcaacuaug ucauacgaau guccaaugcu ugaugaagga      240

guagaaccag augauguaga uuguuggugu aacacaacuu caacaugggu aguauacgga      300

acuugucauc auaagaaagg agaaaccaga cgaagccgaa ggucaguuag ucuaagauau      360

cacuacacaa gaaaacuaca aaccagauca caaacauggc uagaaucaag agaauauaaa      420

aaacacuuaa uuaugguaga gaauuggaua uuucguaauc cagguuuugc aauagugagu      480

guagcaauua cuuggcuuau gggaaguuua acuagucaaa aaguuauaua uuuaguaaug      540

auaguacuaa ucguaccagc auacagcaua ucaugcauag gaguuaguaa cagagaucua      600

guugaaggaa ugucaggagg aacuugggua gacguuguau uagaacacgg aggaugugua      660

acagaaaugg cgcaagacaa accgacaguc gauauagaau ugguaacaau gacaguaagc      720

aauauggcag aaguaagauc auauuguuau gaagcaucac uaucugacau ggcaucggca      780

ucacgaugcc caacacaagg ugaaccaagu uuagauaaac aaagugauac acaaagcguu      840

uguaaaagaa cauuagguga uagagguugg ggaaacgguu guggaauauu uggaaaagga      900

ucacucguaa cauguucuaa auuuaccugu uguaaaaaaa ugccaggaaa auccauucaa      960

cccgaaaauu uggaauauag aauaaugcug cccguacaug gaagucaaca uaguggaaug     1020

auaguaaaug auauaggaca ugaaacagau gaaaaucgug caaaaguuga aguaacacca     1080

aauucaccua gagcagaagc aacguuagga ggauuuggaa gucuaggcuu agauugugaa     1140

ccaagaacag gauuagauuu uuccgauuua uauuaucuaa caaugaauaa caaacacugg     1200

uuaguacaua aagaaugguu ucaugauaua ccauuaccau ggcaugcggg agcugacacu     1260

ggaacaccac auuggaacaa uaaggaagcg cuuguagaau ucaaagaugc acacgcaaaa     1320

agacaaacag uaguaguacu aggaucacaa gaaggagcag uacauacagc auuagccgga     1380

gcacuagaag cagaaaugga cggagcaaaa gguagacuau ucagugguca uuuaaaaugc     1440

agguuaaaaa uggauaaacu ccguuuaaaa ggaguuucuu auaguuuaug uacagcagca     1500

uuuacauuua cuaaaguacc ugcagaaaca uuacauggaa caguaacugu agaaguacaa     1560

ucagccggaa cagauggacc uugcaaagua ccugcacaaa uggcaguaga uaugcaaaca     1620

cuaacaccgg uaggaagacu aauaacagca aaccccguaa uaacugaauc aacagaaaac     1680

ucuaaaauga ugcuagaacu agauccuccu uuuggugacu cauauaucgu gauaggcgua     1740

ggagauaaaa aaaucacaca ucacuggcac agaucaggau cuaccauagg aaaagccuuc     1800

gaagcaacag uaagaggagc aaaaaggaug gcaguguuag gcgauacagc uugggauuuu     1860

ggcagcguag gcggaguauu uaacucacuu ggaaaaggaa uacaucaaau auuuggcgca     1920

gcauucaaau cccuauuugg aggaaugagu ugguuuucac aaauauuaau agguacauua     1980

cuuguauggu uagguuuaaa uacaaaaaac ggcucaauca guuuaacuug ccuagcuuua     2040

ggaggaguaa ugauauuucu aagcacagca gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4899
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 1 opt

<400>  4899
gggagaaagc uuaccaugag uguuggaaua guaggauuau uacuuacaac agcuauggca       60

gcagagguca caagacgagg aucagcuuac uauauguauu uagacaggaa ugaugcuggu      120

gaagcaauau cauucccgac cacccugggu augaacaaau guuacauaca aauuauggau      180

cuuggacaca ugugugacgc aacuauguca uaugaauguc caaugcuaga ugaaggagua      240

gaaccagacg auguagacug cuggugcaac acaacaagca cuuggguagu auauggcacc      300

ugucaucaua aaaaaggaga agcuagaaga aguagaagag caguaacauu accaagccau      360

uccacuagaa aauuacaaac aagaucacaa acaugguuag aauccaggga auauacaaag      420

caccucauaa gagucgaaaa uuggauauuu agaaacccag gauucgcccu ugcagccgca      480

gcaauagccu gguuacuugg aucgagcaca agucaaaaag uuaucuaucu cguaaugaua      540

uuauuaauag caccagcaua uagcauacga uguauuggcg uaucgaauag agauuuugua      600

gaaggaauga gcggaggaac auggguagac gucguucuag aacauggugg augcguaaca      660

guaauggcac aagauaaacc uacaguugau auagaauuag uaacaacaac aguaaguaau      720

auggcagaag uaaggagcua uugcuacgaa gcaucaauca gugauauggc auccgauuca      780

agauguccaa cacaaggaga agcauacuua gauaaacaau cagauacaca auauguaugu      840

aaacguacac uuguagacag aggaugggga aacggaugug gucuauucgg aaaggguagc      900

cuaguaacau gcgcaaaauu cgcaugcagu aaaaaaauga cugguaaaag cauacaacca      960

gaaaaucuug aauauagaau aauguuauca guacauggaa gucaacauuc ugggaugaua     1020

guuaaugaua ccggacauga aacugaugaa aacagagcaa aaguugaaau uacuccaaau     1080

ucaccaagag cagaagcaac auuagguggc uuuggaaguu ugggacuuga uugcgaaccu     1140

agaacaggac uagacuuuag ugauuuguau uacuuaacca ugaacaauaa acauugguua     1200

guacauaaag aaugguucca ugacauucca uuacccuggc augcuggagc ggauaccggg     1260

acaccacauu ggaacaacaa agaagcauua guagaauuua aagacgcaca cgccaaaaga     1320

caaacuguag uaguacuggg aagccaagaa ggagcaguac auacagcgcu agcaggagca     1380

uuagaagcag aaauggaugg ugcaaaagga agauuaagcu caggucauuu aaaaugcaga     1440

cuaaaaaugg acaaacuuag auuaaaaggu guaaguuauu cacuauguac ugcagcuuuc     1500

acauuuacua aaauaccagc agaaacacua cauggcacag uuacuguaga aguccaauac     1560

gcaggaacag auggaccuug uaaaguuccc gcacagaugg ccguagauau gcaaacacuu     1620

acuccaguag gccgacuuau aacagcaaac ccuguaauaa cagaaucaac agaaaauucc     1680

aaaaugaugu uggaauuaga ccccccguuu ggugauaguu acauaguaau aggagucggg     1740

gaaaaaaaaa uaacccauca cuggcaucga uccggcagca caauaggaaa agcauuugaa     1800

gccacuguaa gaggagcaaa aagaauggcg guauuaggag auacagcaug ggauuuugga     1860

agcguaggug gcgcacuaaa uuccuuagga aaagguauac aucaaauauu uggagcugca     1920

uuuaaaucuc uuuuuggggg aaugucaugg uuuagucaaa uuuuaauagg aacucuauua     1980

augugguuag gucucaauac aaaaaacgga ucaauaaguu uaauguguuu agcuuuggga     2040

ggaguccuaa uauuuuuaag cacggcugua agcgcaugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  4900
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 1 opt

<400>  4900
gggagaaagc uuaccaugac aucaguaggu auaguaggac uacuguuaac aacagcaaug       60

gcagcagaag uaacuagaag aggaucggca uauuauaugu aucuugaccg gaaugaugcg      120

ggagaagcua uaagcuuucc aacuacuuua ggcaugaaua aauguuauau ccaaauaaug      180

gaucuaggac auauguguga cgcaacaaug aguuacgaau guccaauguu agacgaaggg      240

guagagccug augacguaga uugcuggugc aacacgacaa guacaugggu cgucuaugga      300

acaugucauc auaaaaaagg agaagcacga agaaguaggc gagcaguaac ucuucccuca      360

cacaguacac gaaaauuaca aacaagaagu caaacauggu uagaaucccg agaguauaca      420

aaacacuuga uaagaguaga aaauuggauu uuuagaaauc caggauuugc acuagcagcc      480

gcagcaaucg cauggcuacu uggaagcagu acaagucaaa aagucauuua uuuagucaug      540

auauuauuaa uugcaccugc auauucaaua agaugcaucg gagugucgaa uagagauuuu      600

guagaaggaa ugaguggagg aacaugggua gauguaguau uagaacaugg aggcugugua      660

acugucaugg cccaagauaa acccacagua gauauagaau uaguaacgac aacaguauca      720

aauauggcag aaguaagauc auacuguuau gaggcuagua uuucagacau ggcaagugau      780

ucuagauguc ccacgcaagg ugaagcauau cuagacaagc aaucagauac acaauaugua      840

uguaaaagga cauuagugga ucgaggcugg ggaaaugguu guggacuuuu ugggaaaggu      900

ucauuaguca caugugcaaa auuugcgugu ucaaaaaaaa ugacaggaaa aaguauccaa      960

ccagaaaacu uagaauacag aauaaugcua ucuguacacg gaucucaaca uaguggcaug     1020

auaguaaaug auacgggaca ugaaacugac gaaaacagag caaaaguaga aauaacacca     1080

aacucaccua gagccgaagc aacauuaggc ggauuuggau cucuaggauu agauugcgaa     1140

ccaagaacug gauuagauuu cucagauuua uauuaucuaa caaugaauaa caaacauugg     1200

uugguacaua aagaaugguu ucacgacauc ccauuaccau ggcaugcagg agccgauacu     1260

ggaacaccac auuggaacaa uaaagaagca cuuguugaau uuaaagaugc acacgcaaaa     1320

cgacaaacug uuguaguucu aggaucgcaa gaaggugccg uacauacagc gcuugcagga     1380

gcacucgaag cagaaaugga uggcgcaaaa ggaagauuaa guucagggca ucuuaaaugc     1440

agacuaaaaa uggauaaauu aagguuaaaa ggaguuucau auaguuuaug cacagcagca     1500

uuuacauuua cuaaaauacc agcggaaaca uugcacggaa cagugaccgu agaagugcaa     1560

uaugccggaa cagacggucc cuguaaagua ccagcucaaa uggcuguaga caugcaaacg     1620

uuaacuccag ucggaagauu aaucacagcg aacccaguaa uaacagaaag uacagaaaau     1680

ucaaaaauga uguuagaauu agacccacca uuuggugaua gcuauauagu aauaggagua     1740

ggugaaaaaa aaauaaccca ucauuggcau aggucuggaa guacaauagg uaaagcauuc     1800

gaagcaacag uaagaggagc uagaagaaug gcuguauugg gagauacugc gugggacuuu     1860

ggaucaguag ggggagcccu uaauucacua ggaaaaggaa uacaucaaau uuuuggagca     1920

gcauucaaau cacuauuugg agguauguca ugguuuagcc aaauucuaau aggaacauua     1980

uuaauguggc uuggauuaaa cacaaaaaau ggaaguauuu cacuaaugug ccuagcacuc     2040

gguggagugu uaaucuuuuu aucaacagca guaucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4901
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 1 opt

<400>  4901
gggagaaagc uuaccaugac uucaguaggu auaguaggau uacuuuuaac uaccgcgaug       60

gcagcagaag uaacaagaag agggucagcu uauuacaugu acuuagacag aaaugaugca      120

ggagaagcaa ucaguuuccc aacuacauua gguaugaaca aauguuacau ccaaauuaug      180

gauuuaggac auauguguga cgcaacaaug uccuacgaau guccaauguu agaugaagga      240

guugaacccg augacgucga cuguuggugc aauacaacaa guacuugggu agucuauggg      300

acaugccauc auaagaaagg agaagcgaga agaagucgac gagcaguaac acuaccaucu      360

cauaguacaa gaaaauuaca aacacgaagu caaacauggc uagaaucuag agaauauaca      420

aaacaucuaa ucagaguaga aaauuggaug uuucgaaauc cuggauuugc acuagccgca      480

gcagcaauag cauggcuauu aggaucauca acaucacaaa aaguaauuua cuuaguaaug      540

auuuuguuaa uagcuccugc auauaguaua agaugcauag gaguaucaaa cagagauuuu      600

guagaaggaa ugucaggagg uacaugggua gauguaguuu uagaacaugg cggaugugua      660

acaguuaugg cacaagauaa accaacugua gauauagaau uaguaacaac uacaguaucu      720

aacauggcug aaguuagauc guacuguuac gaagcaagca uuucugacau ggcaucagau      780

ucuagauguc cuacacaagg agaagcauau cuugauaaac agucagauac acaauacgua      840

uguaaaagaa cauuaguaga uaggggaugg ggaaauggau gcggucuauu ugggaaagga      900

ucguuaguua caugugcaaa auucgcaugu aguaagaaaa ugacaggcaa aucaauacaa      960

cccgagaacc uugaauauag aauaauguua agcguucaug gaucccaaca cagcggaaug     1020

auagucaacg auaccgguca cgaaacugau gaaaaccgug caaaaguaga gauaacucca     1080

aacucaccac gugcagaagc aacauuagga gguuuuggcu cucuaggacu cgacugugaa     1140

cccagaacug gucuagacuu uucagaucua uauuauuuga caaugaauaa caaacauugg     1200

cugguucaua aagaaugguu ccaugauaua ccauuaccau ggcaugccgg agcagauaca     1260

ggaacuccgc acuggaacaa uaaagaggcu uuaguagaau uuaaagaugc ccaugcaaaa     1320

cgacaaacag uaguuguauu aggaagucaa gaaggugcag uucacacagc ucuggcagga     1380

gcauuagagg cagaaaugga cggcgcuaaa gguagauuau cauccggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu aagauuaaaa gguguaagcu auagccuaug cacugcugca     1500

uuuacauuca caaaaauacc agcagaaaca uuacauggaa caguuacggu ugaaguucaa     1560

uaugcaggua cugacggacc auguaaggua ccugcacaaa uggcagugga uaugcaaaca     1620

uugacucccg uugguagguu aauaacagca aauccaguca uaacugaauc aacagaaaau     1680

ucaaaaauga ugcuagaauu agauccucca uuuggagaca guuacauagu aauaggagua     1740

ggugaaaaga aaauaacaca ucauuggcac agaagcggaa guacuaucgg aaaagccuuu     1800

gaagccacgg uuagaggugc aaaaagaaug gcgguauugg gagauacagc augggacuuc     1860

ggcaguguag gaggagcuuu aaauucauua ggaaaaggaa uacaccaaau auucggagcu     1920

gcauuuaaaa gccucuucgg agguauguca ugguuuuccc aaauucuaau aggaacacua     1980

cuaauguggu uaggacuaaa uacuaaaaau ggaucaauau cacuaaugug cuuagcacuc     2040

ggaggaguac uaaucuuccu aucaacagca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4902
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 1 opt

<400>  4902
gggagaaagc uuaccaugac aaguguaggu auaguaggac uauuauuaac aacagcuaug       60

gcagcagaag uaacgcggag agggucagcg uauuauaugu aucucgauag aaacgaugcc      120

ggagaagcaa uaagcuuucc aacaacauug ggaaugaaua aauguuauau acaaauaaug      180

gauuuaggac auauguguga ugcaaccaug ucauacgagu gcccaauguu agaugaaggu      240

guagaaccag augauguuga uuguuggugc aacaugacaa guacaugggu agucuaugga      300

acaugucauc acaaaaaagg agaagcaaga agaucaagaa gagcaguaac acuacccucc      360

cauucaacga gaaaacuuca aacaaggagu caaacauggu uagaaucccg agaauacaca      420

aaacaucuaa uaagaguaga aaauuggaua uuucgaaauc cagguuuugc ucuagcugca      480

gcagcgauag caugguuacu uggaagcucu accucacaaa aaguaauaua uuuaguuaug      540

auucuacuaa uagcaccagc auauucaaua agaugcauag gaguaucaaa ucgagauuuu      600

gucgaaggaa ugucaggagg aacaugggua gauguugucc uagaacaugg aggguguguu      660

acuguaaugg cacaagauaa accuacagua gacauagaac uuguaacaac aacaguauca      720

aauauggcug aaguucguuc auauuguuau gaagcuagua uaagcgacau ggccagcgau      780

ucaagauguc caacacaagg agaagcauac uuagauaaac aaagugauac acaauaugua      840

ugcaaacgaa cucuuguuga uagaggaugg gguaaugguu gcggauuauu uggaaaagga      900

ucacuuguaa caugugcaaa auuugcaugu aguaagaaaa ugacgggcaa aucaauacaa      960

cccgaaaauu uggaauauag aauaauguua aguguucaug gaagccaaca cucaggaaug     1020

auaguaaaug auacaggaca ugaaacagau gaaaaucgag caaaaguaga aauaaccccu     1080

aacagcccac gagcagaagc aacguuagga ggauuuggau cacuaggccu agauugugaa     1140

ccacgaacug gacuugacuu uagcgauuua uacuaccuga cuaugaacaa caaacacugg     1200

uuaguacaua aagaaugguu ucaugacaua ccacuuccuu ggcaugcagg ugcagauaca     1260

ggaacaccac auuggaauaa uaaagaagcu uuaguagaau uuaaagaugc gcaugcaaaa     1320

cgccaaacag uuguaguauu aggcucccaa gaaggagcgg uacauaccgc ccucgcagga     1380

gcauuagaag cagaaaugga uggagccaaa gguagauuau caucuggcca uuuaaaaugu     1440

agguuaaaaa uggacaaauu acgauuaaaa gguguaaguu auucauuaug cacagcugca     1500

uucacauuca caaaaauucc agcagaaaca uuacacggaa caguaacagu ugaaguucaa     1560

uacgcgggaa ccgacggacc auguaaggua ccagcccaaa uggccguaga caugcaaaca     1620

uuaacaccag uaggaagauu aauaacagcu aauccaguaa uaaccgaaag cacagaaaau     1680

ucaaaaauga uguuagaauu agauccucca uuuggagauu cguauaucgu gauuggggua     1740

ggagaaaaaa aaauuacaca ccacuggcau agaucaggcu caaccauagg aaaagcauuu     1800

gaagcuacug uaagaggugc uaaaagaaug gcaguucucg gugacaccgc augggacuuu     1860

ggaaguguag guggugcauu aaauucauua ggaaaaggaa uccaccaaau auuuggagca     1920

gcauuuaaau cguuauucgg aggcauguca ugguuuaguc aaauauuaau agguacuuua     1980

uuaauguggu uaggacuaaa uacaaaaaau gguaguauaa guuuaaugug uuuagcucua     2040

ggaggaguau ugauauucuu aucaacugca guuagcgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4903
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 1 opt

<400>  4903
gggagaaagc uuaccaugac cagcguagga auaguugguc uauuacuaac aacagcuaug       60

gcagcagaag uaacaagaag gggaucagca uauuauaugu auuuagaucg aaaugaugcu      120

ggagaagcca uaucuuuucc cacaacacua ggaaugaaca aaugcuauau acaaauuaug      180

gauuuagguc auauguguga ugcaacaaug ucauacgaau guccuauguu agaugaagga      240

guagaaccgg augauguaga uuguuggugu aauacaaccu caacaugggu agucuacggu      300

acuugucauc auaaaaaagg ugaagcaaga aggaguagga gagcgguaac uuuaccaucg      360

cauucaacaa ggaaacuuca aacaagaagu caaacauggc uagaaaguag agaauauaca      420

aaacaucuca uaagaguuga aaacuggaua uucagaaauc cuggcuuugc uuuggcugca      480

gcagcaauag cgugguuacu agggucauca acaucgcaaa aaguuauaua ccugguuaug      540

auauuauuaa uagcaccggc auauaguauu agauguauag gaguaucaaa uagagauuuu      600

guugaaggaa ugagcggcgg aacaugggua gacguuguuc uagaacaugg aggaugcgua      660

acagcuaugg cucaagauaa accgacagua gacauagaau uaguaacaac uacaguuagc      720

aauauggcug aagucagguc auacuguuac gaagccagca uaagugauau ggcuagcgac      780

aguagauguc ccacucaagg agaagcauau cuagauaaac aaagcgauac acaauaugug      840

uguaaacgua cauuaguaga cagaggaugg ggcaauggau gcggauuauu uggaaaaggu      900

agucuaguaa caugugcaaa auuugccugc agcaaaaaaa ugacaggaaa aaguauacaa      960

ccagaaaacu uagaauaccg aauaauguua ucaguacaug guagucaaca caguggaaug     1020

uuaguaaacg auacagguca ugaaacagau gaaaaucgug caaaaguaga aauaacacca     1080

aauucgccaa gggcagaagc aacucuagga ggauuuggau cacuagguuu ggauugugaa     1140

ccaaggacag gacuagauuu uucagauuua uauuaucuaa cuaugaauaa uaaacauugg     1200

uuagcacaua aagaaugguu ucacgauaua ccauuaccau ggcacgcggg agcagcaacu     1260

ggcacaccac auuggaauaa uaaagaagca uuaguagaau ucaaagaugc acacgcaaaa     1320

aggcaaacug uaguaguacu aggcucacaa gaaggagccg uacauaccgc cuuagcagga     1380

gcauuagaag cugaaaugga uggagcaaaa ggaagauuau cuucuggaca ucuaaaaugu     1440

cgauuaaaaa uggauaaacu aagauugaaa ggaguaagcu acuccuugug uacagcugca     1500

uuuacauuua caaagauccc agcugaaaca guugauggaa cuguaacugu agaaggacag     1560

uauggaggaa cagaugggcc uuguaaaguu ccagcacaaa uggccguaga uaugcaaacg     1620

uuaacuccug uuggaagacu aauaacagca aauccgguaa ucacagaaag cacagaaaau     1680

agcaaaauga uguuagaauu agacccaccu uuuggagauu cauauauagu aauuggugua     1740

ggagaaaaaa aaauaacaca ccacuggcau agaucuggaa guacaauagg aaaagcauuu     1800

gaagcaaccg uaaggggagc gaaaagaaug gcaguucuag gcgauacagc cugggacuuu     1860

ggaagcgucg gaggcgcauu aaauaguuua ggaaaaggaa uacaucaaau uauaggagcu     1920

gccuucaaau cauuauucgg aggcaugucu ugguuuaguc agauauuaau cgguacauua     1980

cuaauguggu uaggauuaaa uacaaaaaac gguagcauau cauuaaugug ucuagcguua     2040

ggaggaguuu uaauuuuucu aaguacagca guaucagggu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4904
<211>  2275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 1 opt

<400>  4904
gggagaaagc uuaccauggg ggcagacgcu ucuauaggaa uaguaagccu auuauuaacu       60

acuguuaugg cagcagaaau aacaagaagg gguagugccu auuauaugua uuuagacaga      120

agugaugcag gaaaagcaau aaguuuuguc acaacuuuag gaguaaauaa augucacguu      180

caaauaaugg auuuaggaca uaugugcgau gcgacaauga guuaugaaug uccuauguua      240

gaugaaggcg uagaaccaga ugacguagau uguuggugua auaccacauc aacuuggguu      300

guauauggaa cuugucauca caaaaaagga gaagcgagaa gaucaaggag agcaguaaca      360

uuaccaaguc auucaacaag aaaacuucaa acaagaagcc aaacaugguu agaaaguaga      420

gaguacacua aacaucuaau aaaaguagaa aauuggauau uuagaaaucc agguuuugca      480

cuaguugcag ucgcuauagc cuggcuacua ggaucaucua caagccaaaa aguaaucuau      540

cuaguaauga ucuuacuaau agcaccagca uauaguauaa gauguauugg aguaagcaau      600

agagauuuug uagaaggaau gagcggagga acuuggguug acguaguauu agaacauggc      660

gguugcguua cagucauggc acaagauaaa ccaacaguag auauugaacu agugacaaca      720

acagugagca auauggcaga aguaagaucg uauuguuaug aagcaucaau aucugauaug      780

gcuucugaua gcagaugccc cacacaaggu gaagcauauu uagacaaaca aagugacaca      840

caauauguau guaaaagaac auuaguagac agaggauggg gaaacggaug uggauuguuu      900

ggaaaaggua guuuaguuac augugcaaaa uucacuuguu caaaaaaaau gacaggaaaa      960

ucuauccaac cagaaaauuu agaauacaga auuaugcuaa guguucaugg aagucaacac     1020

ucgggaauga uagucaauga cgaaaacagg gccaaaguug aaguuacgcc aaacucaccu     1080

agagcagaag ccacacuagg aggauuugga ucacuaggac uugacuguga acccagaacg     1140

ggacuagauu uuagugauuu auauuaucuu acaaugaaua acaaacauug gcuaguacac     1200

aaagaauggu uucaugacau accacuaccc uggcacagug gugcagauac cgaaacacca     1260

cauuggaaca auaaagaagc acuaguagaa uucaaagaug cacaugcaaa gagacagacc     1320

gugguuguac uagggagcca ggaaggagca guacacacgg cauuagcggg agcauuagaa     1380

gcagaaaugg acggagcgaa aggaagauua ucaagcggac aucuaaaaug uagauuaaaa     1440

auggauaaau ugagauuaaa aggaguuuca uacaguuuau guaccgcugc auuuacuuuu     1500

acuaaaguuc ccgcugaaac gcuacacgga acaguaacag uagaaguuca auaugccgga     1560

agagacgguc ccuguaaagu acccgcacaa auggccguag auaugcaaac acuaacaccg     1620

gugggaagac uaauaaccgc caacccaguu auuacagaau caacagagaa uucaaaaaug     1680

auguuagaau uagauccccc auuuggugau ucuuauauag uaauaggagu aggugauaaa     1740

aaaauaaccc accacuggca uaggagcgga ucaauaauag gcaaagcauu cgaagcaaca     1800

guccgaggag caaaaagaau ggcaguacuu ggugauaccg caugggacuu uggaagugua     1860

ggaggaguau uuaauagucu agguaaagga auucaucaaa uuuuuggagc ggcuuuuaag     1920

ucauuauucg gaggaauguc cugguucagu caaauccuaa uaggaaccuu gcuaguaugg     1980

uuaggauuaa auacaaaaaa uggcucaauu ucauuaacau gucuagcacu uggcggagua     2040

augauauuuu uauccacugc aguaucggca ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2220

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2275


<210>  4905
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 1 opt

<400>  4905
gggagaaagc uuaccauggg agccgacacu aguauaggaa uaauaggauu auuauuaacu       60

acagcaaugg cggcugaaau aacucgaaga ggaucagcau auuauaugua uuuagauaga      120

ucagaugcug gaaaagcaau aaguuuugca acaacaaugg gaguaaacaa augccaugua      180

cagauuaugg aucuagguca caugugugau gcuacaauga guuaugaaug cccaauguua      240

gaugagggag uagaaccaga ugauguggau uguuggugua auacaacguc aacauggguu      300

guauauggaa caugucacca caaaaaagga gaagcaaggc cgucaagacg agcaguaaca      360

uuaccuaguc auaguaccag aaaacuacaa acuagaagcc aaacaugguu agaaaguaga      420

gaauauacaa aacacuuaau aaaaguggaa aauuggauac uaaggaaucc aggcuuuacu      480

guagcggcau uagcuauggc auggcuacuu gggaauucaa caucacaacg aguaauauau      540

uuaguaauga uauuacuaau agcgccugca uauuccauaa gaugcauugg aguaagcaac      600

cgcgacuuug uagagggaau gucaggcgga acuuggguug auguaguauu agaacacggu      660

ggauguguaa cuguaauggc acaagacaaa ccuacaguag acauugaauu aguuacgaca      720

acaguaagca auauggcaga aguacguucc uauuguuaug aagcuaguau aucagauaug      780

gcaagcgauu cuagaugucc uacacaggga gaagcauauu uagacaaaca aagugauaca      840

caauacguau gcaaacgcac ccuaguagau cgaggauggg gaaacgggug cggauuauuu      900

gguaaaggau cauugguuac uugcgcgaaa uuuacaugua guaaaaaaau gacaggaaaa      960

ucaauucaac cugaaaauuu agaauaucga auaaugcugu caguacaugg aucacaacau     1020

aguggcauga uagucaauga uauaggacau gaaacagaug aaaacagggc caaaguagaa     1080

gucaccccca auucccccag agcagaagcg acauuaggcg gcuuuggaag uuuaggauua     1140

gauugcgaac ccagaacagg auuagauuuu ucugauuuau auuaucuaac aaugaacaac     1200

aaacacuggu uaguacacaa agaaugguuu cacgacauac cccuaccgug gcaugcagga     1260

gcagauaccg gaacaccaca uuggaauaau aaagaagcau uaguagaauu uaaagaugcu     1320

cacgcaaaaa gacaaacagu uguaguauua ggaucucaag aaggugcugu acauacagca     1380

cuugcaggag cauuagaagc agaaauggau ggagcaaaag gaagauuauu uuccggacac     1440

cuuaaaugca gaauaaaaau ggacaagcua agauuaaagg gaguaaguua uucacuaugu     1500

acugcagcuu uuacauuuag uaaaguacca gcagaaacuu uacaugguac cguaacuguu     1560

gaaguucaau augcaggaag ggaugggcca ugcaaaguac cugcccaaau ggcaguagau     1620

augcaaacuu uaacgccugu agguagauua auuaccgcaa auccuauuau uacagaauca     1680

acugaaaaua gccgcaugau guuagaacua gauccgccau uuggugauag cuauauaguu     1740

auaggaguag gagauaaaaa aauaacacau cauuggcaua gaaguggauc aaccauaggc     1800

aaagcauuug aagcaacagu aagaggagcg aaaagaaugg caguacuagg cgauacugca     1860

ugggacuuug gaucaguagg uggaguauuc aauucauuag gaaaaggagu acaucaaguc     1920

uuuggagcug cuuucaaaag uuuauuugga gggaugucau gguuuagcca aauauuaauu     1980

gggacuuuau uaguuuggcu gggauuaaau acaaaaaaug gaaguauauc auuagcuugu     2040

cuugcacuag gaggagcaau gauauuccua agcacagcug uaucagcaug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  4906
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 1 opt

<400>  4906
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauacu auuaauagca       60

ccagcuuaua gcauaagaug uauaggaguu ucaaacaggg auuucgucga aggaaugagu      120

gguggaacau ggguagauau aguucuagaa cauggagguu guguaacagu gauggcacaa      180

gauaaaccaa cuguagauau agaauuaguu acuacaaccg uaucaaauau ggcagaaguu      240

agaucauauu guuaugaagc aucaauaagu gauauggccu cagauucaag auguccaaca      300

cagggggaag ccuacuuaga uaaacaauca gauacucaau auguauguaa aagaacauua      360

guagauagag guuggggaaa uggaugugga uuauuuggaa aagguaguuu aguaacgugu      420

gcaaaauucg cauguucuaa aaaaaugaca ggaaaaagua uacaaccuga aaaccuagaa      480

uauagaauaa ugcuaucagu acaugguagu caacauucgg gaaugauagu aaaugauaca      540

ggacaugaaa cagacgaaaa uagagcaaaa guugaaauaa ccccaaauag uccaagagca      600

gaagcaacau uaggaggauu uggaucauua ggacuagauu gcgaaccaag aacaggauua      660

gauuuuagug auuuauauua ucuaacaaug aauaauaaac acugguuagu acauaaagaa      720

ugguuccaug auauaccacu accauggcac gcaggugcag auacaggaac accacauugg      780

aauaauaaag aagcauuagu ugaauucaaa gacgcccaug caaaaagaca aacaguagua      840

guacuuggua gucaagaagg ugcuguucac acagcauugg caggagcauu agaagcagaa      900

auggauggag cuaaaggaag auuaucauca ggacauuuaa aaugcagauu aaaaauggau      960

aaacuaagac uaaaaggagu aaguuauagu cuaugcacgg cggcauuuac auucacuaaa     1020

auaccagcgg aaacacuaca ugggacagua acaguagaag uacaauaugc aggaacagau     1080

ggaccuugua aaguuccagc ucaaauggca guagauaugc aaacauuaac accgguaggu     1140

agauuaauaa cagcuaaccc aguaauaaca gaaaguacug aaaauucaaa aaugaugcua     1200

gaacuagauc caccauuugg agacucauau auaguaauag gaguaggaga aaaaaaaaua     1260

acucaucauu ggcacagauc aggaaguaca auaggaaagg cauucgaagc aacaguaaga     1320

ggagcuaaaa gaauggcagu uuuaggagau acagcauggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4907
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 1 opt

<400>  4907
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauacu acucauagca       60

ccagcuuaua guauaagaug cauuggagua ucaaaucgag auuuuguaga agguauguca      120

ggaggaacau ggguagaugu aguacuagaa caugguggau guguaacugu aauggcacaa      180

gacaaaccaa caguagacau agaauuggua acaacaacag uaaguaauau ggcagaagua      240

agaucauauu guuaugaagc aagcauauca gauauggcaa gugacagcag guguccaacu      300

caaggugaag cauaccuaga uaaacaauca gauacacaau auguuuguaa aagaacauua      360

gucgacagag guuggggaaa ugguugcgga cuauucggaa aaggaagcuu aguaacaugu      420

gcaaaauuug cauguagcaa aaaaaugaca gguaaaucaa uucaaccaga aaauuuagaa      480

uacagaauaa ugcuuagugu acacgguucc caacauagug gaaugaucgu aaaugacacc      540

ggacaugaaa cugaugaaaa ucgugcaaaa guagaaauaa caccaaauuc cccaagagcu      600

gaagcaacac uaggaggauu cgguucauua ggauuagauu gugaaccuag aacaggauua      660

gauuuuucag aucuauauua ucuaacgaug aauaauaaac auugguuagu acauaaagaa      720

ugguuucaug auauaccauu acccuggcau gcuggagcag auacagggac accacauugg      780

aauaauaaag aagcguuagu ugaauuuaaa gacgcacaug caaaaagaca aacaguagua      840

guacuuggau cacaagaagg cgcaguacau acagcacuag caggagcacu ggaagcugaa      900

auggauggug caaaggguag acuaucaagc ggacaucuaa aauguagacu aaaaauggau      960

aaacuaagac uaaaaggagu uuccuauagu uuguguacag cagcauuuac uuuuacaaaa     1020

auaccagcag aaacauuaca uggaacagua acaguagaag uucaauaugc aggaacggau     1080

ggaccaugua aaguaccagc acaaauggca guagacaugc aaacauuaac accaguagga     1140

agauuaauaa ccgcuaaucc aguaauaaca gaaucaacag aaaauucaaa aaugaugcuu     1200

gaacuugauc caccauuugg agauucauac auaguaauag gaguaggaga aaaaaaaaua     1260

acacaucacu ggcacagaag uggaucaaca auaggaaaag cauuugaagc aacgguaaga     1320

ggagcaaaaa gaauggcagu auuaggagau acugccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4908
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 1 opt

<400>  4908
gggagaaagc uuaccauguc ucaaaaagua auauauuuag uuaugauauu auuaauagca       60

ccagcauauu caauaagaug uauaggagua ucaaauagag acuuuguaga aggaaugagu      120

ggaggaacau ggguagaugu aguacuagaa cauggaggau guguaacagu uauggcacaa      180

gauaaaccaa cgguagacau agaauuagua acaacuacag uaucaaauau ggcagaagua      240

agaagcuacu guuaugaagc aucaauuagu gacauggcaa gugacagcag auguccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacacaau acguaugcaa aagaacauua      360

guagacagag gauggggaaa uggaugugga uuguuuggca aaggaaguuu aguaacaugu      420

gcgaaauuua cuuguaguaa gaaaaugaca gggaaaucaa uacaaccaga aaauuuagaa      480

uauagaauaa ugcuaagugu acaugguuca caacacucag ggaugauugg uuacgaaacg      540

gaugaagaua gagcaaaagu agaaguuaca ccaaauagcc ccagagcaga agcaacguua      600

gguggcuuug gcaguuuagg auuagauugu gaaccaagaa caggauuaga cuuuagugau      660

cuauauuauu uaacaaugaa uaauaaacau ugguuaguac acaaagaaug guuccaugau      720

auaccucuac cauggcaugc aggagcagau acaggcacac cgcacuggaa uaauaaagaa      780

gcauuaguag aauuuaaaga ugcacaugca aaacgacaaa ccguagucgu auuaggaagu      840

caagaagggg caguucacac agcauuagca ggagcucuug aagcagaaau ggauggagca      900

aaaggaagau uauucucagg acacuuaaaa uguagacuaa aaauggauaa auugagacuc      960

aaagguguau cauauagucu guguacagca gcauucacau uuacaaaagu accugcagaa     1020

acauuacaug ggacaguaac aguugaagua caauaugcag guacugaugg accauguaaa     1080

aucccuguac aaauggcagu ggauaugcaa acucuaacac caguaggaag acuaauaaca     1140

gcuaauccag uaauaacaga aagcacagaa aauagcaaaa ugauguuaga auuagaucca     1200

cccuucggag auaguuacau aguaauagga guuggagaua agaaaauaac acaccacugg     1260

cauagaucag gcaguacaau aggaaaagca uuugaagcua cagugagagg ugcuaaacga     1320

auggcuguau uaggagauac agcuugggau uuuggaaguu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4909
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 1 opt

<400>  4909
gggagaaagc uuaccaugag ccaaaaaguu auauaucuag uuaugauacu auuaauagcc       60

ccagcauaua guauaaggug uauaggggug aguaacagag auuucguaga aggaauguca      120

ggaggaacau ggguagaugu aguacuagaa cacggaggau guguuacagu aauggcacaa      180

gauaaaccaa caguagauau agagcuagua acgacaacag uaaguaauau ggcagaaguu      240

cgaucuuauu guuaugaagc aaguauaagu gauauggcaa gugauucacg uuguccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacucaau auguuugcaa aagaacauua      360

guagauagag gauggggaaa cggguguggu uuauuuggua aaggaaguuu aguaacaugc      420

gcaaaauuug cauguagcaa gaaaaugacu ggaaaaagua uacaaccaga aaauuuagaa      480

uauagaauaa ugcuaagcgu acacgguagu caacauagug guaugauugu caaugauacu      540

ggacaugaaa cagacgaaaa uagagcuaaa guagaaauaa caccaaauag cccacgagca      600

gaagcuacac ucggaggauu ugguucuuua ggauuggauu gugaaccucg aaccggacua      660

gauuuuagcg aucucuacua cuuaacaaug aauaauaaac auuggcuagu acauaaagaa      720

ugguuucaug auaucccauu accauggcau gcaggagcag auacaggaac accacauugg      780

aacaauaaag aagcauuggu agaauuuaaa gacgcacaug caaaaagaca aacuguagua      840

guacuaggau cacaagaggg ugcuguacau acagcgcuag caggcgcacu agaagcagaa      900

auggauggug caaaagggcg auuaaguuca ggacaucuaa aauguagauu aaaaauggau      960

aaauuaagac uaaaaggagu aucauauuca uuauguacug cugcauuuac auuuacaaaa     1020

auaccggcag aaacauuaca uggaacagua acaguagaag uacaauaugc aggaacagac     1080

ggaccaugua aagucccagc acaaauggca guugauaugc aaacauuaac accuguagga     1140

agauuaauaa cagcaaaucc aguaauaaca gaaucaacag aaaacucaaa aaugauguua     1200

gaauuagauc cuccauuugg ugacagcuau auaguaauag guguaggaga aaagaaaauu     1260

acacaccauu ggcauagaag cggaucaacc auaggaaaag cauuugaggc aacaguaaga     1320

ggagcaaaaa gaauggcagu auugggagac acagcauggg auuucggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4910
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 1 opt

<400>  4910
gggagaaagc uuaccaugag ccaaaaagua auauauuuag uaaugauauu acuuauagcu       60

ccagcuuaua guauaagaug uauagggguc ucaaauagag acuuuguuga aggaaugagu      120

ggaggaacau ggguagacgu aguuuuagaa cauggaggau guguaacugu aauggcacaa      180

gauaaaccga caguagauau agaauuagua acaacaaccg uaaguaauau ggcagaagua      240

agaagcuauu gcuaugaagc aagcaucagu gauauggcaa gugauaguag auguccaaca      300

caaggagaag cauaccuaga caaacaaagu gacacacaau auguauguaa aagaacacua      360

guagauagag gauggggaaa cggaugcgga uuauuuggua aagguucauu aguaacaugu      420

gcaaaauuua cauguagcaa aaaaaugaca ggaaaaagca uucaaccaga aaaucuagaa      480

uacagaauca ugcuaucugu acauggauca caacacagug guaugauagu uaaugauacu      540

ggauaugaaa cagaugaaaa uagggcaaaa guggaaguaa cuccaaauag cccaagagca      600

gaagcuacau uaggaggauu cggaaguuua ggacuagauu gcgaaccaag aacaggacua      660

gacuuuucag aucuauauua uuuaacuaug aauaauaaac auugguuagu acacaaagaa      720

ugguuucaug auauaccauu accauggcac gcaggagcag auacuggaac accccauugg      780

aauaauaaag aagcacuagu agaauucaaa gaugcucaug caaaaagaca aacuguagua      840

guauuaggcu cacaagaagg agccgugcau accgcauuag cuggagcucu agaagcugaa      900

auggauggag caaaagggaa auuauucagu ggacacuuaa aaugucgacu aaaaauggau      960

aaguugaggc uaaaaggugu aaguuauuca uuauguacag cagcauucac auuuacuaaa     1020

guaccagcag aaacauugca uggaacggua acaguggaag uccaauaugc aggaacggau     1080

ggaccaugca aaauaccagu acagauggca guagauaugc agacauuaac accaguagga     1140

agacuaauaa cagcgaaucc aguaauaaca gaaaguacag aaaauaguaa aaugauguug     1200

gaauuagauc cccccuuugg agauucauac auaguaauag gaguaggaga uaaaaaaaua     1260

acucaccauu ggcauaggag uggaucaaca aucggaaaag cauuugaagc aacuguaaga     1320

ggagcaaaaa ggauggcagu acuaggagau acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4911
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 1 opt

<400>  4911
gggagaaagc uuaccaugag ucaaaaaguc auauauuuag uaaugauauu auuaauugca       60

ccugcauaua guauaagaug uauaggagua aguaauagag acuuuguaga aggaaugagu      120

gguggaacau ggguagacgu agucuuagaa cauggaggau guguaacugu aauggcacaa      180

gacaaaccaa caguugauau agaauuaguu accacaacag uaaguaauau ggcagaagua      240

aggaguuauu guuaugaagc aaguaucagu gacauggcaa gugauagucg auguccaaca      300

caaggagaag cauaucuaga uaaacaauca gauacacaau auguauguaa aagaacauua      360

guagauagag gaugggguaa cgguugugga cuauuuggua aaggaagcuu aguaacuugu      420

gcaaaauuca cauguucaaa aaaaaugaca ggaaaaucaa uacaaccaga aaaucuggaa      480

uacagaauaa uguuaucagu acauggaagu caacauagug gaaugacagu aaacgauaua      540

ggauaugaaa cagaugaaaa uagagcaaaa guagaaguaa cuccaaauag cccaagggca      600

gaagcaacac uaggagguuu uggaucacug ggauuagauu gcgaaccaag aacuggacuc      660

gacuucagcg auuuauacua uuuaacuaug aauaauaaac auugguuagu ucauaaagaa      720

ugguuucacg acaucccauu accauggcac gcaggagcgg auacagguac accacacugg      780

aacaauaaag aggccuuagu agaauuuaaa gaugcacaug caaaaaggca aacuguagua      840

guauugggaa gucaagaggg agcaguacau acagcacuag caggugcauu agaagcagaa      900

auggacggag caaaagguaa acuauuuuca ggacaucuaa aaugcagauu aaaaauggac      960

aaauuacguu uaaaaggagu aaguuacuca cuuuguacag cggcuuuuac auucacaaaa     1020

guaccagcag aaacauuaca uggcacaguu acaguagaag uacaauaugc aggaacagac     1080

ggaccaugua aaauaccagu acaaauggca guagacaugc aaacacuaac cccgguagga     1140

agacuuauaa cagcaaaucc aguaauaaca gaaaguacug aaaauucaaa aaugaugcua     1200

gaauuagauc cuccauuugg agauucauac auaguaauag guguagggga uaaaaaaauu     1260

acacaccauu ggcauagauc cggaagcaca auaggaaaag cauuugaagc aacgguaaga     1320

ggagcuaaaa gaauggcugu acuaggagau acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4912
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 1 opt

<400>  4912
gggagaaagc uuaccauguc gcaaaaaguu auauaucuag uaaugauauu auuaauagca       60

ccagcuuaua guauaagaug uauaggagua ucaaacagag auuuuguaga aggaaugagc      120

ggagggacau ggguagacgu aguauuagaa cauggagguu guguaacagu aauggcacaa      180

gauaaaccaa cggucgauau agaauuggua acgaccacgg uaucaaauau ggcagaagua      240

agaaguuauu guuaugaagc auccauaagc gauauggcaa gcgauaguag auguccaaca      300

caaggagaag cauaucuuga uaaacaauca gauacccaau auguauguaa aagaacauua      360

guagauagag gauggggaaa ugguugugga uuguucggaa aaggauccuu aguaacaugc      420

gcaaaauuua cauguagcaa aaaaaugacu ggaaaaagua uacaaccuga aaaucuagaa      480

uauagaauaa uguuaucugu acauggaagu caacauucag gaaugauugu aaaugacgaa      540

aauagagcca aaguagaagu gacaccaaau agcccaagag cagaagcaac auuaggaggu      600

uuugguagcu uaggauuaga cugugaacca cgaacagggc uggacuuuuc agacuuauau      660

uaucuaacua ugaauaauaa acauuggcuc guacauaaag aaugguuuca cgauauacca      720

cuaccauggc augcaggagc agauacaggc acaccacauu ggaauaauaa agaagcauua      780

guagaauuca aagacgcaca cgcaaaaaga caaacaguag uaguacuagg aucacaagaa      840

ggagcaguac auacagcuuu agcaggagcu cuagaagcag aaauggaugg agcaaaaggu      900

agacuauuca gcggacauuu aaaauguaga uuaaaaaugg auaaacuaag guuaaaagga      960

guaaguuaua guuuauguac agcagcauuu acauuuacga aaguaccagc agaaacauug     1020

cacggaacag ugacaguuga aguacaauau gcgggaacag acggaccaug caaaguacca     1080

gcacagaugg ccguagauau gcaaacauua acaccaguag gucgacuaau aacugcaaau     1140

ccaguuauua cagaaucuac agaaaauagc aaaaugaugc uagaguuaga uccuccauuc     1200

ggagacaguu auauaguaau aggaguugga gauaaaaaaa uaacacauca uuggcauaga     1260

aguggaagua caauaggaaa agcauuugaa gcaacaguaa gaggagcuaa aagaauggcu     1320

guuuuaggag auacagcaug ggacuuugga aguugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  4913
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 1 opt

<400>  4913
gggagaaagc uuaccauguc acaaaaagua auauacuuag uuaugauacu auuaauagca       60

ccugcuuaca gcauacgaug cauuggugua agcaaucgug acuuuguaga gggaaugagc      120

gguggaacau ggguagacgu aguauuagaa cacggaggau guguaacagu aauggcgcaa      180

gauaaaccaa ccguagauau agaauuaguu acaacaacag uaucaaauau ggcagaagua      240

agaucauauu guuaugaagc aagcauauca gauauggcaa gugacagucg augcccaaca      300

caaggagaag cauauuuaga uaaacaaagc gauacacaau acguauguaa aagaacauua      360

guagauaggg gauggggcaa cgguugcgga uuauucggca aaggaagccu aguuacaugu      420

gccaaauuua cauguaguaa aaaaaugacu ggaaaaucaa uacaaccuga aaauuuagaa      480

uauagaauua ugcuaagugu acacggaagc caacacucag gaaugauugg auacgaaaca      540

gaugaaaaca gggcuaaagu agaagucaca ccaaauucac caagagcaga agcaacacuu      600

ggaggcuucg guucgcuagg acucgauugu gaaccaagaa caggacuaga cuuuucugac      660

uuauauuauc uaacaaugaa uaauaaacau uggcuaguac auaaagaaug guuccacgau      720

auaccauugc cauggcacgc aggugcagau acaggaacac cacauuggaa uaacaaagaa      780

gcauuaguag aauuuaaaga cgcccaugcu aaaagacaaa caguaguagu acuaggaagu      840

caagagggug caguccauac agcauuagca ggagcacuag aagcagaaau ggauggggcu      900

aaagguaaau uauuuagugg acauuuaaaa uguagauuaa aaauggacaa auuaagauua      960

aaaggaguaa guuacagcuu auguacagca gccuucacau uuacaaaagu accagcagaa     1020

acauuacaug gaacaguuac aguagaagua caauaugcag gaacagaugg accauguaaa     1080

auaccagucc aaauggcagu agauaugcaa acauuaacac caguaggaag auuaauaaca     1140

gcaaauccug uaauaacaga aaguacagaa aauagcaaaa ugauguuaga acuagaucca     1200

ccauuuggug acaguuauau agugauagga guuggagaca aaaaaauaac acaucauugg     1260

caucgaucag gaaguacaau uggaaaagca uucgaagcaa cuguaagagg agcaaaaaga     1320

auggcugucu uaggggacac agcaugggau uucggcaguu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4914
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 1 opt

<400>  4914
gggagaaagc uuaccauguc ucaaaaagua auauauuuag uuaugauacu auugauagcu       60

cccgcauaua gucuaagaug uauaggagua agcaauagag auuuuguaga aggaaugagc      120

gguggaacau ggguagaugu aguauuagaa cauggaggau guguaacagu uauggcacaa      180

gauaaaccaa caguagauau agaauuagua acaacaacag uaucaaauau ggcagaagua      240

agaucauauu guuaugaagc aaguauaagc gauauggcuu cagauaguag augcccaacc      300

caaggagaag cauaccuaga uaaacaaagu gauacacaau acguauguaa aagaacguua      360

guagauagag gauggggaaa uggaugcgga uuauuuggaa aaggaagucu aguaacaugu      420

gcuaaauuug cauguucaaa gaaaaugaca ggaaaaucaa uucaaccaga aaauuuagaa      480

uauagaauaa uguuaagcgu acaugguuca caacauagug gaaugaucgu aaaugauaca      540

ggacaugaaa cagaugaaaa ucgagcuaaa guagaaauaa caccuaacag uccaagagca      600

gaagcaacau uaggaggauu uggaaguuua ggauuggauu gcgaaccaag aacagguuua      660

gauuucucag auuuauauua ucuaacaaug aauaacaaac auugguuggu acauaaagaa      720

ugguuucaug auauaccucu accuuggcau gcaggagcag acacgggaac accacacugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcucaug caaaaagaca aacuguagua      840

guauuaggaa gucaagaagg agcaguucau acagccuuag caggagcauu agaagcagaa      900

auggauggag cuaaaggaag auuaucaagu ggacauuuga aaugcagauu gaaaauggau      960

aaauuaagau uaaaaggagu aucauauagu cuauguacag cagcauuuac auuuacaaaa     1020

auaccagcgg aaacacuaca uggcacaguu acugucgaag uacaauaugc aggaacagac     1080

gguccaugua aaguaccagc acaaauggca gucgauaugc aaacauuaac accaguggga     1140

agauuaauaa cagcaaaucc aguaauaacu gaaucaacug aaaauucaaa aaugaugcua     1200

gaauuagauc cacccuucgg ugauucguac auaguaauag gaguugggga aaagaaaaua     1260

acacaucauu ggcauagauc aggaaguaca auuggaaaag cauucgaagc uacuguuaga     1320

ggcgcuaaaa gaauggcagu auugggagau acagcauggg auuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4915
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 1 opt

<400>  4915
gggagaaagc uuaccaugag caaaaaagua auauaccuag uaaugauauu acuaauagca       60

ccagcauaca guauacgaug uauaggagua aguaacagag auuuuguuga aggaauguca      120

ggaggaacau ggguugaugu aguauuagaa cacggcggau guguaacagu aauggcacaa      180

gauaaaccaa caguugauau agaauuagua accacaacag uuagcaauau ggcugaagua      240

agaucauauu guuaugaagc aucaauauca gauauggcaa gugauucaag auguccaaca      300

caaggugaag cauaucuaga caaacaauca gauacacaau acguauguaa aagaacacua      360

gucgauagag gauggggaaa uggaugcggu uuauucggaa aagggucacu aguuacaugu      420

gccaaauuug caugcagcaa aaaaaugacu ggaaaaucaa uucaaccaga aaaucuugaa      480

uauagaauaa uguuaucagu acaugguuca caacauagcg gaaugauugu uaacgacacc      540

ggacaugaaa cagaugaaaa ucgagcaaaa guagaaauaa cuccaaacuc accaagagcu      600

gaagcaacau ugggaggauu cggaucauua ggacuagacu gugaaccaag aaccggauua      660

gauuuuucag acuuauauua uuuaacaaug aauaacaaac auugguuagu acacaaagaa      720

ugguuucacg acauaccacu accauggcac gcaggagcug auacagguac accacauugg      780

aauaacaaag aagcucucgu agaauuuaaa gaugcacaug caaaacgaca aacuguaguc      840

guauuaggau cucaagaagg agcuguacau acagcacuag caggagcauu agaagcagaa      900

auggauggag cuaaaggaag auuaagcuca ggccaucuca aaugccgauu aaaaauggau      960

aaauuaagau uaaaaggagu aucauauuca uuauguacag cagcauucac auuuacaaaa     1020

auuccagcug aaacauuaca ugguacugua acaguagaag uacaauaugc cgguacagau     1080

gguccaugua agguaccugc acaaauggca guagauaugc aaacauuaac accaguagga     1140

agacuaauaa cagcaaaucc uguaauaaca gaaaguacag aaaauucaaa aaugaugcua     1200

gaauuagacc caccguucgg cgauaguuau auuguaauag gaguaggaga aaaaaaaaua     1260

acucaccauu ggcauagauc aggcucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcuaaaa gaauggcugu ucuaggagac acagcauggg auuuugguuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4916
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 1 opt

<400>  4916
gggagaaagc uuaccaugag ccaaaaagua aucuauuuag ucaugauauu auugauagcc       60

ccagcauacu cuauaagaug uauaggagua aguaauagag acuucgucga aggaaugagu      120

ggaggaacau ggguagaugu aguucuagaa cauggaggau guguaacagu aauggcucaa      180

gacaaaccca caguagauau agaauuagua acaacaacag uaagcaauau ggcagaagua      240

cgaaguuauu gcuaugaagc aucaauauca gauauggcau cagauucaag auguccaacc      300

caaggagaag cuuaucucga uaaacaauca gauacacaau auguauguaa aagaacucua      360

guagauaggg gauggggaaa ugguugcggu cuauuuggaa aaggaaguuu agucacaugu      420

gcaaaauuug caugcaguaa aaaaaugaca ggaaaaucua uacaaccaga gaacuuagaa      480

uauagaauaa uguugagugu acacgguagu caacauagug guaugauagu caaugauaca      540

ggacaugaaa cagaugaaaa uagagcaaag guagaaauaa cgccaaauuc accaagagca      600

gaagcaacau uaggaggauu uggaagcuua ggacuagacu gugaaccacg aacgggauua      660

gauuuuagug accuauauua uuuaacaaug aauaacaaac auuggcuggu ucauaaagaa      720

ugguuucacg auauaccacu accauggcau gcuggagcag auacagguac accacacugg      780

aacaauaaag aagcauuagu agaauuuaaa gacgcucaug caaaaagaca aacgguagua      840

gucuuaggau cacaagaagg agcaguacac acagcauuag cgggagcacu agaagcagaa      900

auggauggug caaaaggaag auuaaguagu ggacaucuaa aauguagauu aaaaauggau      960

aaauuaaggu uaaaaggagu aaguuauuca uuauguacgg cggcauuuac auuuacaaaa     1020

auaccugcag aaacguuaca uggaacaguu acagucgaag uucaauaugc uggaacagau     1080

ggacccugua aaguuccagc acaaauggcg guagauaugc aaacauuaac accaguugga     1140

agauuaauca cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugaugcuu     1200

gaauuagauc caccauucgg agauagcuau auaguaauug gaguagguga aaaaaaaaua     1260

acgcaucauu gguauagauc aggaucaaca auaggaaaag cauuugaagc aacuguaagg     1320

ggagcaaaac guauggcagu auugggagau acagccuggg acuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4917
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 1 opt

<400>  4917
gggagaaagc uuaccauguc acaaaaagua auauaucuag uaaugauauu acuuauagcu       60

ccugcauaua guauacgaug cauaggggug ucaaauagag auuuuguaga aggaaugagu      120

gggggaacau ggguagaugu aguacuagaa cacggagguu guguaacagu cauggcacaa      180

gauaaaccga cuguggauau agaacuugua acaacuacag uaucaaauau ggcagaagua      240

agaaguuauu gcuacgaagc aaguauaagc gacauggcaa gcgauuccag guguccaaca      300

caaggagaag cauaccuaga uaaacaaagc gauacucaau auguauguaa aagaacgcuc      360

guagauagag gcuggggaaa uggaugugga uuauuuggua aagggaguuu aguuacaugu      420

gcuaaauuug caugcaguaa gaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa      480

uaucgaauaa uguugucagu ucauggaagu caacauucag gaaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa uagagcgaaa guugaaauaa caccaaauuc accaagagca      600

gaagcaacac uagggggauu ugggucacua ggacuagauu gugaaccaag aacaggauua      660

gauuuuucag aucucuacua ccuaacaaug aacaauaaac acugguuagu acauaaagaa      720

ugguuucacg acaucccauu accauggcau gcaggagcag auacaggaac gccacauugg      780

aauaacaaag aagcauuagu agaauuuaaa gaugcccaug cuaaaagaca aacaguugua      840

guacuugguu cacaagaagg ugcaguacac acagcauuag caggagcauu agaagcagaa      900

auggauggag caaaaggaaa acuaagcucu ggacaucuaa aaugcagacu aaaaauggau      960

aaauuaagau uaaaaggagu aagcuauagu cuaugcacag cagcauucac uuucacaaaa     1020

auaccagcag aaacauuaca cggaacagua acgguagaag uacaauaugc aggaacugac     1080

ggaccaugua aagugccagc acaaauggca guagauaugc agacauuaac accaguaggu     1140

agauuaauaa cagcaaaccc ggugauuacu gaaagcacug aaaauaguaa aaugauguua     1200

gaacuagauc caccuuuugg agauaguuau auaguuauag gaguaggaga aaagaaaaua     1260

acacaccacu ggcacagauc aggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcugu acuaggagau acagcauggg acuuugguag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4918
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 1 opt

<400>  4918
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauacu ccucaucgca       60

ccagcauacu caauaagaug uauaggagua ucaaacagag acuuuguaga aggaaugucc      120

ggaggaacuu ggguugaugu uguauuagaa cauggaggau guguaaccgu aauggcacaa      180

gacaaaccaa caguagauau ugaacuagua acaacaacag uaucaaauau ggcagaaguu      240

agaaguuauu guuacgaagc aucaauauca gauauggcau cagauucaag gugcccaaca      300

caaggagaag cauauuuaga caaacaauca gacacacaau auguaugcaa aagaacacug      360

gucgauagag gauggggaaa cggaugugga uuguucggaa aaggaucauu aguuaccugu      420

gcaaaauucg caugcagcaa aaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa      480

uacagaauaa uguuaagugu ucacggauca caacauagug gaaugauugu uaacgacaca      540

ggacacgaaa cagaugaaaa uagagcaaaa guagaaauaa cgccaaauuc gccaagagca      600

gaagcaacau uagguggauu uggaagcuua gguuuagauu gugaaccaag aacaggacuu      660

gauuuuagcg auuuauacua ccuaacaaug aauaauaaac auugguuagu ucacaaagaa      720

ugguuucaug auauaccauu accauggcac gcaggagcag auacaggaac accacacugg      780

aauaacaaag aagcacuugu cgaauuuaaa gaugcgcacg caaaaagaca aacaguaguu      840

guucuaggau cacaagaagg agcaguacau acagcucuag cuggagcacu agaagcagaa      900

auggacggag caaaaggcag auuauccagu ggacauuuaa aauguagacu aaaaauggac      960

aaauuaagac uuaaaggagu uaguuauuca cuauguacag ccgcauucac auucacaaaa     1020

auuccagcag aaacacuaca uggaacagua acagucgaag uacaauaugc aggaacagau     1080

ggaccaugua aaguaccggc acaaauggcu guagacacuc agacauuaac accaguagga     1140

agguuaauua cagcaaaucc aguaauaacu gaaucaacag aaaauagcaa aaugauguua     1200

gaauuagauc caccauuugg ugauaguuau auuguuauag gaguaggaga aaaaaaaaua     1260

acacaucauu ggcaucgauc aggaagcaca auaggaaaag cauuugaagc cacuguaaga     1320

ggagcaaaaa gaauggcagu acucggugau acagcguggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4919
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 1 opt

<400>  4919
gggagaaagc uuaccauguc acaaaaagua auauaccuag uaaugauauu auuaauagcu       60

ccagcauaua guauaagaug cauaggagua aguaaucgag auuuuguaga gggaaugagc      120

ggggguacau ggguagacgu aguauuggaa cacggaggau guguaacagu aauggcacaa      180

gacaaaccaa caguagacau ugaacugguu acgaccacug uaucaaauau ggcagaaaua      240

agaucauacu gcuaugaagc gaguauaagu gauauggcau cagauucuag auguccaaca      300

caaggagaag cauaucuaga caaacaaagu gauacacagu auguauguaa aagaacgcua      360

guagauagag gauggggaaa uggauguggu cuauuuggga aagguagcuu aguaacaugu      420

gcaaaauuug caugcaguaa aaaaaugaca ggaaaaucaa uucaaccaga aaauuuagaa      480

uauagaauaa uguuaucugu gcauggauca caacauucag gaaugauagu gaacgauaca      540

ggacacgaaa cugaugaaaa uagagcaaaa gucgaaauaa ccccaaauuc acccagagcc      600

gaagcaacau uaggaggauu uggaucacua ggccuugauu gugaaccaag aacaggauua      660

gacuuuucag acuuguauua ucuaacaaug aauaacaaac auugguuagu acauaaagaa      720

ugguuucaug auauaccauu accuuggcau gcaggagcag auacggguac accacauugg      780

aauaauaaag aagcccucgu agaauuuaaa gaugcacaug cuaaaaggca aacuguugua      840

guacuaggua gucaagaagg agcuguacau acagcauuag caggagcauu agaagcagaa      900

auggacgggg caaaagguag gcucaguuca ggacaccuaa aaugcagauu aaaaauggau      960

aaauuaagac uaaaaggcgu aaguuauagu uuauguacag cagcauuuac auucacaaaa     1020

auaccagcag aaacguuaca uggaacagua acaguagaag uacaauaugc aggaacagau     1080

ggaccuugca aaguuccagc acaaauggca guagauaugc aaacacuaac accaguagga     1140

agacuaauaa ccgcaaaucc aguaauaaca gagagcacag aaaauagcaa aaugaugcua     1200

gaauuagacc caccauucgg agauuccuau auaguaauag gaguagguga aaaaaaaauu     1260

acacaucacu ggcaccgauc aggaucaaca auagguaagg cuuucgaagc aacaguaaga     1320

ggagcuaaac guauggcagu auuaggagau acugcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4920
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 1 opt

<400>  4920
gggagaaagc uuaccauguc acaaaaagua auauacuuag uuaugauuuu guuaauagca       60

ccagcauauu caauaagaug cauuggagua ucaaauagag auuucguaga aggaaugagc      120

ggaggcacau ggguagaugu aguauuagaa caugguggau guguuacugu aauggcacaa      180

gauaaaccaa cuguagacau agaacuagua acaacaacag uaucaaauau ggcagaagua      240

agaaguuauu guuaugaagc aagcauaucc gauauggccu cagacaguag auguccaaca      300

caaggagaag cuuacuuaga uaaacaauca gauacacaau auguauguaa aagaacacua      360

guagacagag gauggggaaa uggaugcggu uuauucggaa aaggaagucu aguaacaugc      420

gcaaaauuug cauguaguaa aaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa      480

uacagaauaa ugcuaagugu ccauggaagu caacauagug gaaugauugu aaaugacaca      540

ggacaugaaa cagaugaaaa uagagcgaaa guagaaauaa caccaaacuc accaagagca      600

gaagcaacau uaggaggauu uggaucauua ggucuugauu gugaaccaag aacuggacuu      660

gauuuuucag auuuauauua ccuaacaaug aauaauaaac acuggcuggu acauaaagaa      720

ugguuccaug acauaccucu acccuggcac gcaggagcag auacagguac cccacauugg      780

aauaauaaag aagcauuagu agaguucaaa gaugcacaug caaaaagaca aacaguaguu      840

guauuaggau cacaagaagg agcaguacau acagcacuag caggagcauu agaagcagaa      900

auggacggcg caaaaggcag acuuagcagu ggacaccuaa aaugccgcuu aaaaauggau      960

aaacuaagau uaaaaggagu aaguuauuca uugugcacag cagcauucac auucacaaaa     1020

auacccgcag aaacacuaca cgguacaguu acaguugaag uacaauaugc aggaacagau     1080

ggaccaugua aaguuccagc acaaauggca guagauaugc aaacauuaac accaguaggu     1140

cgauuaauaa cagcaaaccc aguaauuaca gaaucaacag aaaauaguaa aaugauguua     1200

gaacucgacc ccccauucgg agacucauac auaguuauag gaguagguga aaaaaaaauu     1260

acacaucacu ggcacagauc aggaucaaca auaggaaaag cuuuugaagc aacaguuaga     1320

ggagcacgaa gaauggcugu auuaggagau accgcguggg acuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4921
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 1 opt

<400>  4921
gggagaaagc uuaccauguc ucaaaaaguu auauauuuag uaaugauuuu auuaauagca       60

ccagcauauu caauuagaug uauaggagua ucaaauagag auuuuguaga gggaaugucu      120

ggaggaacau ggguagaugu aguucuagaa cauggaggau guguaaccgu aauggcgcaa      180

gauaaaccaa cugucgauau agaauuagua acaacaaccg uuucaaauau ggcagaagua      240

agaucauauu guuaugaagc aucaauaagu gauaugguaa gcgacucaag auguccaaca      300

caaggagaag cauaucugga uaaacaaucg gacacgcaau auguauguaa aaggacucua      360

guugauagag gauggggaaa uggaugcggu uuauuuggua aaggaaguuu aguaacaugc      420

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaucca uacaaccuga aaauuuagaa      480

uacagaauaa uguuaucagu acauggaagu caacauucag gaaugauagu aaacgacaca      540

ggacaugaaa cagaugaaaa cagagcuaaa guggaaauaa caccgaauag cccaagagca      600

gaggcaacau uaggaggauu uggaucacug ggauuagauu gugaaccaag aacaggcuua      660

gauuuuagcg acuuauacua uuuaacaaug aauaauaaac auuggcuagu gcauaaagaa      720

ugguuccacg auauaccauu accguggcau gcaggagcag auacaggaac accacauugg      780

aauaacaaag aagcuuuagu agaauuuaaa gacgcccaug caaaaagaca aacgguagua      840

guauugggau cacaagaagg ugcaguacau acagcacuag caggagcauu agaagcagaa      900

auggauggcg caaaaggaag acuaucguca ggacacuuaa aauguagauu aaagauggac      960

aaacuuagau uaaaaggcgu aaguuauuca uugugcacag cagcauuuac auuuacaaaa     1020

auaccugcag aaacauuaca uggaacagua acuguagaag uacaauaugc aggaacagac     1080

gguccaugca aaguaccagc acaaauggca guagauaugc aaacacuaac accaguagga     1140

agacuaauaa ccgcaaaucc agucauaaca gaaucaacag aaaauagcaa aaugaugcua     1200

gaauuagauc cuccauucgg agauagcuau auagucauag ggguugggga aaaaaaaauu     1260

acucaccacu ggcauagaag uggaagcacc auaggaaaag cauuugaagc aacaguaaga     1320

ggagccaaaa gaauggcagu guuaggagau acggcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4922
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 1 opt

<400>  4922
gggagaaagc uuaccaugag ucaaaaagua auauaccuag uaaugauacu auuaauagca       60

ccugcauaua guauaagaug uauaggagua ucaaauagag auuucguaga aggaauguca      120

ggaggcacau ggguagacgu aguuuuagaa cauggaggau guguaacugu aauggcacaa      180

gauaaaccua cuguagauau agaauuagua acaacaacag uaucaaacau ggcagaagua      240

agaaguuauu guuaugaagc aagcauaucu gauauggcau cagacucaag augcccuaca      300

caaggagaag cguacuuaga caaacaaagc gauacacaau auguauguaa aagaacacuc      360

guagauagag gaugggggaa cggauguggu uuauucggga aaggaucccu aguaacaugu      420

gcaaaauuug cauguaguaa aaaaaugaca gggaaaucaa uacaaccuga aaaccuagaa      480

uauagaauaa uguuaagcgu acauggauca caacauucag gaaugauugu aaacgacaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauua caccaaauuc accaagggcu      600

gaagcaacau uaggagguuu uggaaguuua ggauuagauu gugaaccaag aacaggacua      660

gauuucagug auuuguacua cuuaacaaug aauaauaaac auuggcuugu ucauaaagaa      720

ugguuccaug auauaccacu accauggcac gcaggagcag auacuggaac accucacugg      780

aacaauaaag aagcacuagu ugaauucaaa gacgcccaug caaaacgaca aacugucgua      840

guauuaggau cacaagaagg agcaguacau acagcauuag caggagcauu agaagccgaa      900

auggauggag cuaaaggaag auuaucauca ggacaucuaa aguguaggcu aaaaauggac      960

aaacuaagau uaaaaggagu gaguuacagu uuauguacag ccgcauuuac auuuacaaaa     1020

aucccagcag aaacacugca uggaacugua acaguugaag uacaauaugc aggaacagau     1080

gggccaugca aaguaccagc acaaauggca guagauaugc aaacacuaac accaguagga     1140

agguuaauaa cagcuaaucc aguaauaaca gaaaguauag aaaauaguaa aaugauguua     1200

gaacuagacc caccuuucgg ugauucuuau auaguaauag gaguagguga aaaaaaaaua     1260

acacaucauu ggcauagauc uggaaguacc auaggaaaag cauuugaagc uacaguaaga     1320

ggagcuaaaa gaauggcagu auuaggagau acagcauggg auuucgguag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4923
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 1 opt

<400>  4923
gggagaaagc uuaccauguc acaaaaagua auauaccuag uaaugauauu acuaauagca       60

ccagcuuacu caauaagaug uauaggggua ucaaauagag auuuugucga aggaaugucu      120

ggaggaacau ggguagaugu cguacuugaa cacggaggau guguaacagu aauggcacaa      180

gauaaaccaa caguagauau agaauuaguu acaacaacag uaucaaacau ggcugaagua      240

agaucauacu guuacgaagc cucaauuuca gauauggcaa gugauucuag guguccaacg      300

caaggagaag ccuauuuaga uaaacaaucc gauacacaau auguuuguaa aagaacuuua      360

guagauagag gaugggguaa cggaugugga uuauucggaa aagggucucu aguaacaugu      420

gcaaaauuug cauguaguaa aaaaaugaca ggaaaaucaa uacaaccuga aaaucuagaa      480

uacagaauaa uguuaagugu acauggaagc caacauucag gaaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa ccgugccaaa guagaaauaa caccaaacuc accacgagca      600

gaagcaacuu uaggaggauu ugggucacua ggacuagacu gugaaccaag aacuggacuu      660

gacuuuucug aucuauauua uuuaaccaug aauaacaaac acuggcuugu acacaaagaa      720

ugguuccaug auaucccauu accauggcac gcaggagcag auacaggaac accccauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaacgaca aacagucguu      840

guauuaggau cacaagaagg agcaguacau acagcacuag caggagcacu agaagcagaa      900

auggauggag caaaaggaag auuaucaagc ggacaccuaa aauguagacu aaaaauggau      960

aaauuaagau uaaaaggagu aaguuauagu uuauguacag cagcauucac auucacaaaa     1020

auaccagcag aaacacuaca uggaacaguc acaguagaag uacaauaugc uggaacagau     1080

ggaccuugua aaguaccagc acaaauggca guagauaugc aaacauuaac uccaguagga     1140

agauuaauua cagcaaaucc uguaauaaca gaaucaaccg aaaacucaaa aaugauguua     1200

gaauuagacc caccauuugg agacucauau auagucauag gaauaggcga aaaaaaaaua     1260

acacaucacu ggcacagaag uggcucaaca auaggaaaag cauuugaagc gacaguaagg     1320

ggagcaaaaa gaauggccgu auuaggagac accgcauggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4924
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 1 opt

<400>  4924
gggagaaagc uuaccauguc gcaaaaagua auauaucugg uuaugauuuu guuaauagca       60

uuagcauacu caauaagaug cauaggagua aguaauaggg auuuuguaga aggaaugagu      120

ggagguacau ggguagaugu aguauuagaa caugguggau gcguaacagu aauggcacaa      180

gauaaaccua caguagacau agaauuagua accaccacag uuucaaacau ggcagaaguc      240

agaucauacu guuacgaagc aagcauaucc gauauggcau cagacucuag auguccaacc      300

caaggugaag cauauuuaga caaacaauca gacacacaau auguauguaa acgaacuuua      360

guagauagag gauggggaaa ugguugcgga uuauuuggaa aaggaucauu aguaacaugu      420

gcaaaauucg caugcaguaa aaaaaugacu ggaaaaucaa uacaacccga aaauuuagaa      480

uauagaauaa uguuaagugu ucauggcagu caacauagug guaugauagu aaaugacaca      540

ggacaugaaa cagaugaaaa cagagcaaaa guugaaauaa caccaaacuc uccaagagca      600

gaagcaacau uaggagguuu ugguaguuua ggacuagauu gugaaccaag aacuggauua      660

gauuuuucag aucuauauua uuugacaaug aauaauaaac auugguuagu ucacaaagaa      720

ugguuccaug auauaccacu accauggcau gcaggagcag auacaggaac accacauugg      780

aauaauaaag aagcauuagu ggaauuuaaa gaugcacaug caaaacgaca aacaguaguu      840

guacuaggau cacaagaagg agcaguacac acggcauuag caggagcauu agaagcagaa      900

auggaugggg caaaaggaag guuaagcucc gggcauuuaa aaugccgauu aaaaauggac      960

aaauuaagau uaaaaggugu gucuuauagu cuauguacag cagcauuuac auuuacaaaa     1020

auaccggcag aaacacuuca ugguacagua acaguagaag uacaauaugc aggaacagau     1080

ggcccaugua aagugccagc acaaauggca guagacaugc aaacacuaac accagucgga     1140

agauuaauaa cugcaaaucc aguaauaaca gaaucaacag aaaacaguaa aaugauguua     1200

gaauuagacc cuccguucgg agacuccuau auaguaauag gaguagguga aaaaaaaaua     1260

acacaucauu ggcauagauc aggaucaaca auaggaaaag ccuuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu acuaggagac acagcauggg acuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4925
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 1 opt

<400>  4925
gggagaaagc uuaccauguc acaaaaagua auuuacuuag uuaugauacu acuuauagca       60

ccagcauauu caauaagaug uauaggagua ucaaauaggg acuuuguaga aggaaugagu      120

ggugguacuu ggguagacgu aguauuagaa cacggaggau guguaacugu aauggcgcaa      180

gauaaaccaa caguagacau agaauuaguu acaaccaccg uaaguaauau ggcagaagua      240

agaaguuauu gcuacgaagc aaguauauca gacauggcaa gugauucaag auguccaacu      300

caaggugaag cauauuuaga uaaacaauca gauacacaau auguauguaa aagaacucua      360

guagacagag gaugggguaa uggaugcggc uuauucggaa aaggauccuu aguuacaugu      420

gcuaaauuug cuuguaguaa gaaaaugaca ggaaaaagua uucaaccaga aaacuuagaa      480

uacagaauaa uguugagcgu acaugguagu cagcauucag gaaugauagu caaugauaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccaaauag uccaagagca      600

gaagcgacau uaggaggauu uggaagccua ggacuagacu gugaaccaag aaccgguuua      660

gauuucuccg auuuauacua uuuaacaaug aacaacaaac acuggcuagu ccauaaagaa      720

ugguuucaug auauuccacu accauggcau gcaggagcag acacggguac accacacugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacaug caaagagaca gacaguaguu      840

guauuaggaa cacaagaagg ugcaguacac acagcacuag cuggugcacu agaagcagaa      900

auggauggag caaaaggccg acuaaguagu ggacaccuua aauguagauu aaaaauggau      960

aaauuaagau uaaaaggagu uucuuauagu uuauguacag cagcauucac auuuacaaaa     1020

auaccagcug aaacauuaca cggaacagua acaguugaag uucaauaugc aggaacagau     1080

ggaccaugua aaguaccugc gcaaauggcu guagauaugc aaacguuaac accaguagga     1140

agauuaauaa cugcaaaccc aguuauaaca gaaucuacag aaaauaguaa aaugauguua     1200

gaauuagacc cuccauuugg cgauaguuau auaguaauag gaguaggaga aaagaaaaua     1260

acacaccacu ggcauagauc aggaaguaca auaggaaaag cauuugaagc uacaguacga     1320

ggcgcuaaaa ggauggcugu auuaggugau acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4926
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 1 opt

<400>  4926
gggagaaagc uuaccaugag ccaaaaaguu auauauuuag uuaugauacu auuaauagca       60

ccagcuuaca guauaagaug uauaggagua aguaauagag auuuuguaga ggguauguca      120

ggaggaacau ggguagacgu aguauuagaa cauggaggau guguaacagu aauggcacaa      180

gauaaaccaa caguagauau agaacuagua acgacaacag uaucaaauau ggcugaaguu      240

aggucauauu guuaugaagc aaguauuagu gacauggcau cagauagcag auguccaaca      300

caaggagaag cauaucuaga caaacaaagu gauacacaau auguaugcaa aagaaccuua      360

guagauagag gauggggaaa cggcuguggu cuauuuggaa aaggaucauu aguaacaugc      420

gcaaaauuug caugcaguaa aaaaaugaca ggaaaaucaa uacaacccga aaaucuagaa      480

uauagaauaa uguuaagugu acauggauca caacacucgg gcaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa uagagcgaaa guagaaauaa caccaaauag uccuagagcc      600

gaagcaacgu uaggaggauu uggcucacuu ggacuggauu gugaaccaag aaccggacua      660

gauuuuucag auuuauauua uuuaacaaug aacaauaaac auugguuagu acacaaagaa      720

ugguuucaug auauccccuu accauggcac gcaggugcag auacaggaac accacauugg      780

aauaauaaag aggcauuagu agaauuuaaa gacgcacaug caaaaagaca aacaguagua      840

guauuaggaa gucaagaagg agcaguacau acagcauuag cuggagcccu agaagcggaa      900

auggacggag caaaaggaag guuaucaagu ggacauuuaa aauguagacu aaaaauggau      960

aaauuaagac uaaaaggagu aucauacagc uuguguacag cagcauuuac auuuacaaaa     1020

auaccagcag aaacauuaca cgguacaguu acaguagaag uacaauaugc aggaacagac     1080

ggaccuugca aaguauuagc acaaauggcc guagauaugc aaacacuaac accaguagga     1140

agauuaauaa cagcaaaucc aguaauaaca gaaagcacag aaaacaguaa aaugauguua     1200

gaacucgauc cacccuucgg agauuccuac auagucauag gugugggaga aaaaaaaauu     1260

acacaucauu ggcauagaag cggaagcaca auaggcaaag cauuugaagc aacagucaga     1320

ggagcaaagc gaauggcagu ucuaggagac acagccuggg auuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4927
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 1 opt

<400>  4927
gggagaaagc uuaccaugag ccaaaaagua auauauuuag uaaugauauu acuaauagca       60

ccagcauaua guauacgcug uauuggagua ucgaaucgag acuuuguaga aggaaugagc      120

ggaggaacau ggguagaugu aguacuagaa cacggaggau guguuaccgu uauggcacag      180

gauaaaccaa caguagauau agaauuagua acaacaacag uuaguaauau ggcagaagua      240

agaagcuauu guuacgaagc aucaauaagc gacauggcaa gcgauaguag augcccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacucaau auguaugcaa acgaacauua      360

guagacagag gauggggaaa uggaugugga uuauuuggaa aaggaagucu aguuacaugc      420

gcaaaauuug cuuguagcaa gaaaaugaca ggaaaaucaa uacaaccaga aaacuuggaa      480

uauagaauaa uguuaucugu acauggauca caacauucag gaaugauagu aaacgauacu      540

ggacacgaaa cagaugaaaa uagagcaaaa guugaaauua cacccaauag cccaagagca      600

gaagcaacau uagggggauu cggaucauua ggauuagacu gugaaccaag aacaggauua      660

gacuucucag auuuguauua uuuaacaaug aauaauaaac acuggcuagu acacaaagaa      720

ugguuucaug auauaccucu cccauggcac gcaggagcug auacaggaac accacacugg      780

aauaacaaag aagcauuagu agaauuuaaa gaagcacaug caaaaagaca aacuguugua      840

guacuaggaa gucaagaagg agcaguacac acugcauuag caggagcuuu agaagcggaa      900

auggauggag caaaaggaag auuaucaagu ggacaucuaa aauguagacu aaaaauggau      960

aaacuaagau uaaaaggcgu aaguuauagu cuauguacgg cugcguucac auucacaaaa     1020

auaccagcag aaacauuaca cggaacagua acaguagaag uacaauaugc aggcacagau     1080

ggacccugca aaguaccagc acaaauggca guagauaugc aaacauuaac accaguagga     1140

agacuaauaa cagcaaaucc uguaauaaca gaaaguaccg aaaauaguaa aaugauguua     1200

gaacuagauc caccauuugg agauaguuau auaguaaucg gaguuggaga aaagaaaaua     1260

acucaucacu ggcacagaag cgguaguacc auagguaaag cauuugaagc uacagugcga     1320

ggagcaaaaa gaauggcagu uuuaggagau acagcauggg auuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4928
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 1 opt

<400>  4928
gggagaaagc uuaccauguc acaaaaagua auauacuuag uuaugauauu acucaucgcu       60

ccggcauaua guauaagaug uauuggagua ucaaauagag auuuuguaga aggaauguca      120

ggaggaacau ggguagaugu gguauuagaa cauggcggau gcguaacagu aauggcacaa      180

gauaaaccaa caguagacau agaauuaguc accacaacag ucagcaauau ggcagaagua      240

agaagcuauu gcuaugaagc aucaauaagu gauauggcaa gugauagcag auguccaaca      300

caaggagaag cuuaucuaga uaaacaaagu gauacacaau acguauguaa aagaacauua      360

guugacagag gauggggaaa cggcugugga cuauuuggaa aaggaaguuu aguaacaugc      420

gcaaaauucg cauguagcaa aaaaaugaca ggaaaaagua uucaaccaga aaaccuagaa      480

uauagaauaa uguuaagcgu acauggaagu caacauucag gaaugauagu uaacgauaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa ccccaaauag uccuagagca      600

gaagcagcau uaggaggauu uggaucacua ggauuagauu gcgagccaag aacaggguua      660

gauuuuucag auuuauauua ucuaacaaug aauaacaaac auugguuagu acacaaagaa      720

ugguuccacg auauaccauu accauggcac gcaggcgcgg acacaggaac accccauugg      780

aacaauaaag aagcauuagu agaauuuaaa gaugcgcaug caaaacgaca aacuguagua      840

guacuuggaa gucaagaagg agcugugcau acagcucugg caggagcuuu agaagcagaa      900

auggauggag caaaaggaag auuaucaagu ggucacuuaa aauguagauu aaaaauggau      960

aaacuaaggu uaaaaggugu gagcuacagu uuaugcacag cugcauuuac auuuaccaaa     1020

auaccugcag aaacacuaca cggcacaguu acuguagaag uccaauaugc aggaacagac     1080

ggaccaugua aagugccagc acaaauggca guagacaugc aaacauuaac accagugggg     1140

agacuaauaa cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugauguua     1200

gaauuagauc caccauucgg agauucauau auagucauag guguggguga aaaaaaaauu     1260

acacaucauu ggcauagauc aggaucaacg aucgguaaag cauuugaagc aacaguacgu     1320

ggggcgaaac gaauggcagu auuaggagau acggcauggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4929
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 1 opt

<400>  4929
gggagaaagc uuaccauguc acaaaaagua auauaucuag uaaugauauu auuaauagca       60

ccggcauaua gcauaagaug uaucggagua aguaauagag acuuuguaga aggaaugagu      120

ggaggaacau ggguugaugu aguacuagaa cauggaggau gcgugacagu aauggcucaa      180

gauaaaccaa caguagauau agaacuagua agcacaacag uaaguaauau ggcagaaguu      240

agaagcuauu guuacgaagc aacaauaagu gauauugcaa gugacaguag auguccaaca      300

caaggagaag cauaucuaga uaaacaaagu gauacacaau auguauguaa aagaacgcuu      360

guagauagag gauggggaaa uggcugugga cuauuuggaa aaggaagccu aguaacaugu      420

gcaaaauucg caugcaguaa aaaaaugaca ggaaaaagua uacagccaga aaauuuagaa      480

uacagaauaa ugcuaagugu acauggauca caacacagug gaaugauagu aaacgauaca      540

ggacaugaaa cagaugaaaa cagagcaaaa guagaaauaa caccgaauag cccgagggcu      600

gaagcaacau uaggaggauu uggaagcuua ggauuagauu gugaaccaag aacaggauua      660

gauuuuagug acuuauacua uuuaacaaug aauaauaaac acugguuagu acauaaagaa      720

ugguuccaug acauaccccu accuuggcau gcaggugcag auacaggaac accacauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcucaug cuaaaagaca aacagcaguu      840

guacuaggau cacaagaagg agcaguacau acagcacucg caggggcauu agaagcagaa      900

auggacggag caaaaggcag auuaucaagc gggcauuuaa aauguagacu aaaaauggau      960

aaauuaagac uaaaaggugu gucauauuca cuauguacag cugcauucac auuuacaaaa     1020

auaccagcag aaacauuaca cggaacgguc acagucgaag uccaauacgc aggaacagau     1080

ggaccaugua aaguacccgc acagauggca guagauaugc aaacacuuac accaguaggu     1140

agauuaauaa cagcaaaucc aguaauaaca gaaaguacag aaaacaguaa aaugaugcua     1200

gaauuagauc caccauuugg agauucauau auaguaauug gagugggcga aaaaaaaauu     1260

acacaucacu ggcaucgaag uggaucaaca auaggaaaag cauuugaagc aaccguaagg     1320

ggagcuaaaa ggauggcagu cuuaggagac acagcuuggg auuucgguuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4930
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 1 opt

<400>  4930
gggagaaagc uuaccaugag ucaaaaagua auauaucuag ugaugauauu auuaauagca       60

ccagcauaua gcauaagaug uauaggagug ucaaacagag auuucguaga aggaauguca      120

ggaggaacau ggguagaugu gguacuagaa cauggagguu gcguuaccgu aauggcacaa      180

gauaaaccaa caguagacau agaauuagua acaaccaccg uaaguaauau ggcagaaguu      240

agaaguuauu guuaugaagc aagcauaagc gacauggcaa gcgacagcag auguccaaca      300

caaggagaag cauauuuaga uaaacaaagc gacacacaau auguaugcaa aagaacuuua      360

guagauaggg guuggggaaa uggcugugga cuauuuggaa aagguaguuu aguaacaugu      420

gcaaaauuug cauguucaaa gaaaaugacu ggaaaaucaa uacaaccaga aaauuuagaa      480

uaucguauaa ugcuaagugu acauggauca caacacagug gaaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccaaacuc acccagagcu      600

gaagcaacgu uaggaggauu uggaucauua ggauuagauu gugaaccaag gacaggauua      660

gauuuuagug aucuauauua ucuaacaaug aauaauaaac acugguuagu ucauaaagaa      720

ugguuucaug auauaccauu accauggcau gcaggagcag acacaggaac accacauugg      780

aacaauaaag aagcgcuagu ggaauuuaaa gaugcacacg caaaaagaca aacuguaguu      840

guacuaggaa gucaagaagg ggcaguacau acggcucuag cuggagcacu ggaagcagaa      900

auggacgggg cuaaagguag auuaucgagc ggacauuuaa aauguagguu gaaaauggac      960

aaacuuagau uaaaaggugu aucauauagu uuauguacag cagcguuuac guuuaguaaa     1020

auaccugcag aaacauuaca cggaacagua acagaagaag uacaauacgc aggaacggau     1080

ggucccugca aaguuccagc acaaauggcu guggacaugc aaacauuaac accuguagga     1140

agauuaauaa cagcaaaccc aguaauaaca gaaagcacag aaaauaguaa aaugauguua     1200

gaauuagauc caccauuugg agauaguuau aucguaauag gaguaggaga aaagaaaauc     1260

acucaucacu ggcauagaag cgguaguaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu acuaggagau acagcguggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4931
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 1 opt

<400>  4931
gggagaaagc uuaccauguc acaaaaagua auuuauuuag uuaugauauu acuaauagca       60

ccagcauauu caauaagaug uauaggagug aguaaucgag auuuuguaga aggcaugagu      120

ggaggaacuu ggguagaugu aguuuuagaa cacggcggau gcguaacagu aauggcacaa      180

gauaaaccga cgguagacau agaauuagua acaacaacag uuaguaacau ggcagaagua      240

agaucauauu guuaugaagc aaguauaagu gauauggcua gugauucaag auguccaaca      300

caaggcgaag cauacuuaga uaaacaaagc gauacacaau auguauguaa gagaacauua      360

guagauagag guuggggaaa uggaugugga uuauuuggaa aaggaucacu aguaaccugc      420

gcaaaauuug cuuguucaaa aaaaaugaca ggaaaaucaa uccaaccaga aaaucuagaa      480

uauagaauaa uguuaucagu acaugggagc caacacucgg gaaugauagu aaaugauacu      540

ggccacgaaa cagacgaaaa ucgagcaaaa guagaaauaa caccaaauuc accaagagca      600

gaagcaacac uaggaggauu uggaucauua ggauuagacu gugaaccaag aacuggacua      660

gauuuuucag accuauacua ucuaacaaug aauaauaaac acugguuagu acauaaagaa      720

ugguuucaug acauaccacu gccauggcau gcaggcgcag auacgggaac gccacauugg      780

aacaauaaag aagcauuagu ugaauuuaaa gaugcacaug cgaaaagaca aacuguagua      840

guauuaggaa gccaagaagg agcaguucac accgcauuag cuggagcguu agaagcagaa      900

auggacggag caaaagggag auuaucaagc ggucaucuaa aaugcagacu aaaaauggau      960

aaauuaagau uaaaaggagu aagcuauucg cuauguacag cagcauuuac auuuacaaaa     1020

auaccagcag aaacauuaca uggaacagua acaguagaau uacaauaugc uggcacagau     1080

ggaccaugua aaguaccagc acaaauggca guagauaugc aaacucuuac accaguagga     1140

cgauuaauua cagcaaaucc aguaauaacu gaaucuacug aaaacaguaa aaugauguua     1200

gaauuagauc caccauuugg agauaguuau auaguaauag gaguaggaga aaaaaaaaua     1260

acacaucauu ggcauagauc agguaguaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu uuuaggugau acagccuggg auuuugguuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4932
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 1 opt

<400>  4932
gggagaaagc uuaccaugag ucaaaaagug aucuauuuag uaaugauucu acuaauagca       60

ccagcauauu caauaagaug uauaggcaua ucuaacaggg auuuuguuga aggaaugagu      120

ggaggaacuu ggguagacgu aguauuggaa cauggaggau guguaacagu aauggcacaa      180

gauaaaccua caguagacau agaacuggua acaacaacag uaaguaauau ggcugaagua      240

aggucauacu guuacgaagc aaguauaagc gauauggcaa gugauucaag augcccaaca      300

caaggagaag cauaucucga uaaacaaagc gauacacaau auguauguaa aagaacauua      360

guagauagag gauggggaaa cgguugcgga uuauuuggga aaggaucuuu aguaacaugu      420

gcaaaauucg cauguaguaa aaaaaugaca ggaaaaucca uacaaccaga aaacuuagaa      480

uauagaauaa uguuaagugu acacgguagu caacauucag gaaugauugu aaaugauaca      540

ggacacgaaa ccgacgaaaa uagagcaaaa guagaaauaa caccaaacuc accaagagca      600

gaagcaacac uaggaggauu uggaauacuu ggauuagauu gugaaccacg aacuggacua      660

gauuuuucag accuauauua ucuaacgaug aauaauaaac auuggcuagu acauaaagaa      720

ugguuucaug auauacccuu accauggcau gcaggcgcag auacgggaac gccacauugg      780

aacaacaaag aagcauuagu agaauuuaaa gaugcacaug caaaacgaca aacaguagua      840

guauuagggu cacaagaagg agcaguacau acugcacuug caggagcuuu agaagcagaa      900

auggacggug caaaaggaag auuaucauca ggacacuuaa aauguagauu aaaaauggac      960

aaauuacgau uaaaaggagu aucauauagc uuaugcaccg cagcauuuac auuuacuaaa     1020

auaccugcug aaacauuaca uggaaccgua acaguagaag uacaauaugc aggaacagau     1080

ggaccaugua aaguaccagc acaaauggca guagauaugc aaacacuaac accaguagga     1140

agacuuauca cagcaaaucc aguaauaaca gaaagcacgg agaauucaaa aaugauguua     1200

gaacuagauc caccauuugg agacucauac auaguaauag gaguaggaga aaaaaaaaua     1260

acacaucauu ggcacagauc aggaaguacu auaggaaaag cauuugaagc aacgguaaga     1320

ggagcaaaga gaauggcagu auuaggagau acagcauggg auuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4933
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 1 opt

<400>  4933
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauauu auuaauagca       60

ccagcauaua guauaagaug uauaggagua aguaauagag auuucguaga aggaaugagu      120

gggggaacau ggguagaugu aguauuagaa cauggagggu guguaacagu aauggcacaa      180

gacaaaccaa ccguggauau ugaauuagua acuacuacag ucaguaauau ggcagaaguc      240

agaucauauu guuacgaagc uaguauauca gacauggcga gugauagcag auguccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacucaau auguauguaa aagaacauua      360

guugauagag gguggggaaa uggaugugga uuauuuggaa aaggaagcuu aguaacaugu      420

gcaaaauucg cauguaguaa aaaaaugaca ggaaaaagua uacaaccaga aaacuuagaa      480

uauagaauaa ugcugagugu acauggauca caacauucag gaaugauagu aaaugauaca      540

ggacaugaga cagaugaaaa cagagcaaag guagaaauaa cuccaaacag uccacgagca      600

gaagcaacac uagguggauu uggaucacua ggacuagauu gugaaccuag aacaggauua      660

gauuuuucag auuuauauua ucuuacaaug aacaacaaac auuggcuagu acauaaagaa      720

ugguuccacg auauaccacu accauggcau gcuggagccg auaccggcac uccacauugg      780

aacaauaaag aagcauuagu agaauucaaa gaugcacacg cuaaaagaca gacaguggua      840

guauuaggaa gucaagaagg agcaguacac acagcacuag caggagccuu agaagcagaa      900

auggaugggg uaaaagguag guuaagcagu ggacacuuaa aauguagauu aaaaauggau      960

aaacuaagac uuaaaggugu aaguuacuca uuauguacag cagcuuuuac guuuacuaaa     1020

aucccugcag aaacacuaca uggaacagua accguggaag uacaauacgc aggaacagau     1080

ggaccaugua aaguaccagc acaaauggcu guagauaugc aaacauuaac cccaguagga     1140

agauugauaa cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugauguua     1200

gaauuagauc cucccuuugg ugauagcuau auaguuauag guguaggaga aaaaaaaaua     1260

acacaucauu ggcacagauc aggcagcaca auagguaaag cuuuugaagc aacaguaaga     1320

ggagcaaaac gaauggcagu guugggagau acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4934
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 1 opt

<400>  4934
gggagaaagc uuaccaugag ccaaaaagua auuuauuuag uaaugauacu auuaauagca       60

ccagcauaua guauaagaug uauaggggua uccaacagag auuucguuga aggaaugagc      120

ggaggaacau ggguagaugu aguuuuagaa caugguggau guguaacagu aauggcacaa      180

gauaaaccua caguagauau agaauuagua acaacaacag uaucaaacau ggcagaagua      240

agaaguuauu gcuaugaagc aagcauuagu gauauggcaa gugauagucg auguccaaca      300

caaggagaag cauaucuaga caaacaaagu gacacacaau acguauguaa aagaacauua      360

guagauagag gaugggguaa uggcugcgga cuauucggga aaggauccuu aguaacuugu      420

gcaaaauuug ccuguaguaa aaaaaugaca ggaaaaucaa uacaacccga aaaucuagaa      480

uaccgaauaa ugcuaagugu acacggaagu caacauucag gaaugaucgu gaaugacaca      540

gggcaugaaa cugaugaaaa cagagcaaaa guagaaauua caccaaauuc accaagggcu      600

gaagcaacau uaggaggauu cggaaguuua ggacuggauu gugaacccag aacuggauua      660

gacuuuucag auuuauacua uuuaacaaug aauaacaaac acuggcuagu acauaaagaa      720

ugguuucacg auauaccauu accauggcau gcaggagcag auacaggaac accacauugg      780

aauaauaaag aagcacuagu agaauuuaaa gaugcacacg cuaaaagaca aaccguugua      840

guauuaggau cacaagaagg agcgguucac acagcacuag ccggagcauu agaagcggaa      900

auggauggag caaaaggaag auuaucauca ggucacuuaa aauguagguu aaaaauggau      960

aaacuaagac uaaaaggagu aagcuauagu cuuuguacag cagcauucac auucacaaaa     1020

auaccagcag aaacauuaca uggaacagua acaguugaag uacaauaugc aggagcagac     1080

ggaccaugca aaguaccugc acaaauggca guggauaugc aaacauuaac uccaguaggu     1140

agguuaauaa cagcaaaccc aguaauaaca gaaaguacgg aaaauucaaa aaugaugcua     1200

gaauuagauc caccauuugg agauaguuau auaguaauag gaguaggaga aaaaaaaauu     1260

acacaccauu ggcauagauc agguaguaca auaggaaaag cauuugaagc aaccguacgu     1320

ggagcuaaaa gaauggcugu auuaggagau acagcauggg acuuugguag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4935
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 1 opt

<400>  4935
gggagaaagc uuaccauguc acaaaaagua auauaucuag uuaugauacu auuaauagca       60

ccagccuaca guauacgaug uauuggagua aguaauagag auuuuguuga aggaaugagu      120

gggggaacau ggguagaugu gguacuugaa cauggaggau gugucacagu aauggcacaa      180

gauaaaccaa caguggauau agaauuagua acaacuacag uaaguaacau ggcagaagua      240

agaaguuauu gcuacgaagc uucaauaagu gacauggcaa gugauagcag auguccgaca      300

caaggcgaag cauaucuaga uaaacaaagc gauacucaau auguauguaa aagaacauua      360

guagacagag guuggggcaa uggaugugga uuauuuggua aaggcucucu aguaacaugu      420

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaucaa uucaaccaga aaaucuagaa      480

uauagaauaa uguuaucagu ccauggaagu caacauucag guaugauagu aaacgacaca      540

ggacaugaaa cagaugaaaa cagagcaaaa guagaaauca caccaaauag uccaagagcc      600

gaagcaacuu uaggaggauu cggaucauua ggauuagauu gcgaaccaag aaccggguua      660

gauuuuagug auuuauauua ucuuacuaug aauaauaaac auuggcuagu acauaaagaa      720

ugguuucaug auauaccauu accuuggcac acuggagcag auacaggaac accacauugg      780

aacaauaaag aagcuuuagu agaauuuaaa gaugcacaug caaaaagaca aacaguagua      840

guauuaggaa gucaagaagg agcuguacau acugcauuag cuggugcauu agaagcagaa      900

auggauggag caaaaggaag gcuaagcagu ggacaucuaa aauguagauu aaaaauggau      960

aaacuaagau uaaaaggagu aucauauagu cuauguacag cagcauucac auuuacaaag     1020

auaccagcag aaacacuaca cgguacagua acgguagaag uccaauaugc aggaacagau     1080

ggcccuugua aagugccagc acaaauggca guagauaugc aaacacuaac uccaguagga     1140

agacuuauaa cagcaaaucc aguuauaaca gaaggcacag aaaacaguaa aaugauguua     1200

gaacucgauc caccauuugg cgauucauau auaguaauag gaguaggaga aaaaaaaaua     1260

acacaucauu ggcauagguc uggaucaacu auaggaaaag cauuugaggc aacuguaaga     1320

ggagcaaaaa gaauggcagu uuuaggugau acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4936
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 1 opt

<400>  4936
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauauu guuaauagca       60

ccagcguaca guauuagaug uauaggaguu agcaauagag auuuuguaga aggaaugagu      120

ggaggaaccu ggguagaugu aguauuagaa caugggggau guguaacagc aauggcgcaa      180

gauaaaccaa caguagacau agaauuagua acaacuaccg uuaguaauau ggcagaagua      240

cgaagcuauu guuacgaagc uucaauaagu gauauggcaa gugacagcag auguccaaca      300

caaggagaag cguaccuaga uaaacaaagu gauacacaau acguaugcaa aaggacuuua      360

guagauagag gauggggaaa uggauguggu uuauuuggga aaggaagccu uguaacaugu      420

gcaaaauuug cauguagcaa aaaaaugaca gguaaaucaa uacaaccuga aaaucuagaa      480

uauagaauaa uguuaagcgu acauggauca caacauagcg gaauguuagu aaacgauaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa cgccaaacuc accucgagcc      600

gaagcaacau uagguggauu uggaucauua ggauuagauu gcgaaccaag aacaggcuua      660

gacuuuagcg auuuauauua cuuaacgaug aacaauaaac auugguuagc ucauaaagaa      720

ugguuucaug auauaccacu accauggcac gcgggagcag caacaggaac gccucauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacaug cuaaaagaca aacaguagua      840

guacuaggaa gccaagaagg agcaguacac acagcauugg caggagcauu ggaagcagaa      900

auggauggag caaaaggacg auuaaguucu ggacauuuaa aaugucgauu aaaaauggau      960

aaacuaagac ucaaaggggu aucauacuca uuauguacag cagcguuuac auucacaaaa     1020

auaccagcag aaacaguaga ugguacagua acaguagaag gacaauaugg uggaacagac     1080

ggaccaugca aagugccagc acaaauggca guagauaugc aaacacuuac accaguagga     1140

agauuaauaa cagcaaaucc agugauaaca gaaucgacag aaaacaguaa aaugauguua     1200

gaacuagauc caccuuuugg agauucauau auuguaauag gaguagggga aaaaaaaaua     1260

acacaucauu ggcauagauc uggaagcacg auaggaaaag cuuuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu uuuaggggau acagcauggg auuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4937
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 1 opt

<400>  4937
gggagaaagc uuaccaugag ccaaaaaguu auauaccuag uaaugauauu auuaauagca       60

ccagccuaua guauacgaug cauuggagua ucaaauagag auuucguaga aggcauguca      120

gguggaacau ggguagaugu aguauuagaa cauggaggau guguaacagu aauggcacaa      180

gacaaaccag caguagauau agagcuagua acaacuacag uaucaaauau ggcagaagua      240

agaaguuacu guuaugaagc uagcauaagc gauauggcau cugauucuag guguccaaca      300

caaggcgaag cauauuuaga uaaacaaucu gauacacaau acgucuguaa aagaacucua      360

guagauagag gauggggaaa cggauguggu uuauucggaa aaggcagcuu aguuacaugu      420

gcaaaauuug caugcucaaa gaaaaugaca ggaaaaucaa uacaaccuga aaaccuagaa      480

uacagaauaa uguuaucagu gcacggauca caacauagcg gaaugauagu caaugauaca      540

ggacaugaaa cagaugaaaa cagagcuaaa gucgaaauaa caccaaacag uccaagagcu      600

gaagcaacau uaggaggauu cgguagcuua ggacuugauu gcgaaccaag aacaggacua      660

gauuuuucag aucuuuauua cuuaaccaug aacaacaaac auugguuggu acacaaagaa      720

ugguuucaug auauaccacu accuuggcau gcaggagcag auacuggaac accacauugg      780

aacaauaaag aagcauuagu agaauuuaaa gacgcucaug caaaaagaca aacagucguu      840

guauuaggau cacaagaagg agcaguacau accgcucuag cuggagcauu agaagcagaa      900

auggauggag caaaaggaag auuaucauca ggacacuuaa aaugcagacu aaaaauggac      960

aaauuaagac ucaaaggagu aucuuauuca uuaugcaccg cagcauuuac auuuacaaaa     1020

auaccagcag aaacacuaca uggaacugua acagucgaag uccaauaugc aggaacagau     1080

ggaccgugca aagucccggc acaaauggca guagacaugc aaacuuuaac accaguagga     1140

cgauuaauaa cugcaaaucc uguaauaaca gaaagcacag aaaauucaaa aaugaugcua     1200

gaauuagauc cacccuucgg agauucauau aucguaauag gaguaggaga aaagaaaaua     1260

acacaucauu ggcauagauc aggaagcacu auagguaaag cauuugaagc aacaguaaga     1320

ggugcaaaaa gaauggccgu uuuaggugau acagcauggg auuuuggcag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4938
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 1 opt

<400>  4938
gggagaaagc uuaccaugag ucaaaaagua auauauuuag ugaugauacu auuaauagca       60

ccagcauaua guauaagaug uauaggugug aauaauagag auuuuguuga aggaauguca      120

gggggaacau ggguagaugu cgucuuagaa cacggagguu guguaacagu gauggcacaa      180

gauaaaccaa caguagauau agaauuagua acaaccacag uaaguaauau ggcagaagua      240

agaucauauu guuaugaggc aaguauaagu gauauggcaa gugauaguag auguccgaca      300

cagggagaag cauauuuaga uaaacaaagc gauacucaau auguauguaa aagaacauua      360

guagauagag gauggggaaa uggaugcgga uuguuuggaa aaggaagucu agugacaugu      420

gcaaaauuug caugcuccaa aaaaaugaca ggaaaaucaa uacaaccaga gaacuuagaa      480

uauagaauaa uguuaagugu acauggaagu caacacagcg gaaugauagu uaaugauaca      540

ggacaugaaa cagaugaaaa ucgagcgaaa guugaaauaa cuccaaacuc accuagagca      600

gaagcaacau uaggaggguu cggaucauua ggacuagauu gugaaccaag aacaggacua      660

gauuucagug acuuauauua ucuaacaaug aauaauaaac auuggcuagu acauaaagaa      720

ugguuucaug auauacccuu accauggcau gcgggugcag auacagguac accacauugg      780

aauaauaaag aagcccuugu ggaauuuaaa gaugcacacg caaaaagaca aacaguaguc      840

guacuagguu cacaagaagg agccgugcac acagccuuag caggagcacu ggaagcagaa      900

auggaugggg caaaaggaag auuaucgagu ggacaucuaa aaugcagacu aaaaauggau      960

aaauuaagau uaaaaggagu aaguuauuca uuauguacag cagcauuuac auuuacaaaa     1020

auaccagcag aaacauuaca uggaacagua acaguugaag uacaauaugc gggaacagac     1080

ggaccaugua aaguaccagc acaaauggca guagacaugc aaaccuuaac cccagucgga     1140

agacuaauaa cagcaaaccc gguaauaacc gaaaguacag aaaacucuaa aaugauguua     1200

gaacuagauc caccauuugg agauaguuac auaguaauag gagugggaga aaagaaaaua     1260

acacaucacu ggcauagaag cggaagcaca auuggcaaag cauucgaagc aaccguaaga     1320

ggagcaaaaa gaauggcagu auuaggagau acugcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4939
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 1 opt

<400>  4939
gggagaaagc uuaccauguc acaaaaagua auauauuuag ucaugauauu gcuaauagca       60

ccugcauaua guauaagaug uauaggagua aguaauagag acuuuguaga aggaaugagc      120

ggcggaacau ggguagacgu aguacuagaa cauggaggau guguaacagu aauggcacaa      180

gauaaaccaa caguagauau agaauuagua acaacaacag uaucuaacau ggcugaagua      240

agaaguuauu gcuacgaagc aaguauaagc gauauggcau cagauuccag auguccgaca      300

caaggagaag cauauuuaga uaaacaaagc gauacacaau augugugcaa aagaacauua      360

guagauagag gauggggaaa uggaugcgga cuauuuggaa aagguagcuu aguaacaugu      420

gcaaaauuug cauguucuaa aaaaaugaca ggaaaaagca uacaaccaga aaauuuagaa      480

uaucgaauaa uguuaagcgu acauggauca cagcauucag gaaugauagu aaacgauaca      540

ggucaugaaa cagacgaaaa uagagcaaaa guagaaauaa caccuaacuc cccaagagca      600

gaagcaacau uaggaggauu uggaucacua ggauuagauu gugaaccaag aacuggacuu      660

gacuucagug acuuauauua uuuaacaaug aauaauaaac auugguuagu acauaaagaa      720

ugguuucacg acauaccauu accauggcau gcaggagcag auacaggaac uccccauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaaagaca aacaguagua      840

guacuuggaa gucaagaagg agcaguacau acagcuuuag caggagcacu ggaagcagaa      900

auggacggug cuaaaggaag acuuucaagu ggacaucuaa aaugucgguu aaaaauggau      960

aaauuaagau uaaaaggcgu aaguuauagc uuaugugcag cagcauuuac uuuuacaaaa     1020

auaccugcag aaacacuaca cgguacaguu acgguagaag uccaauaugc uggaacggau     1080

ggaccaugua aaguaccugc acaaauggca guagauaugc agacauuaac uccaguggga     1140

agauuaauca cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugaugcua     1200

gaauuagauc caccauucgg ggauaguuau auaguaauag gaguagguga aaaaaaaaua     1260

acacaucacu ggcauagauc cggaucgaca auaggaaaag cauuugaagc aacaguaaga     1320

ggggcuaaaa gaauggcagu auuaggagau acagcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4940
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 1 opt

<400>  4940
gggagaaagc uuaccauguc acaaaaagua auauaccuag uaaugauauu auuaauagca       60

ccagcauaua gcauaagaug uauaggagua aguaauagag acuuugucga aggaaugagu      120

ggaggaacau ggguagacgu aguauuagaa cauggaggau guguaacagu uauggcacaa      180

gauaaaccaa caguagacau agaacuagua acaacuacag uuucaaauau ggcugaagua      240

agaaguuauu guuacgaagc aaguauuagc gauauggcga gugauucaag augcccaacu      300

caaggagaag cauaucuaga uaaacaaagu gauacacaau auguauguaa aagaacacua      360

guagacagag gauggggaaa uggaugugga uuauuuggaa aaggaucauu aguuacaugu      420

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaagua uacaaccuga aaauuuggaa      480

uauagaauaa ugcugagugu acauggauca caacacagug gaaugauagu caaugauauu      540

ggacaugaaa cagaugaaaa ucgagcaaaa guagaaauaa caccgaauag cccaagagca      600

gaagcaacuu uaggaggauu uggaucauua gggcuagauu gugaaccuag aacuggacua      660

gauuuuagug acuuguauua cuuaacaaug aauaacaaac acuggcuggu acauaaagaa      720

ugguuucaug auauaccauu accauggcac gcaggugcag acacaggaac accacauugg      780

aauaauaaag aagcauuagu agaauucaaa gaugcacaug caaaaagaca aacaguggua      840

guauuaggaa gucaagaagg agcaguacau acugcauuag caggugcuuu agaagcagaa      900

auggauggag caaaaggaag auuaucuuca ggacauuuaa aaugcagauu aaaaauggac      960

aaauuaaggu uaaaaggagu aaguuacagc cuuuguacag cagcauuuac uuuuacgaaa     1020

auccccgcag aaacuuuaca uggaacagua acaguagaag uacaauaugc aggaacagac     1080

ggaccaugca aaguaccagc acaaauggca gucgauaugc aaacauuaac accuguagga     1140

agacucauaa cagcgaaucc gguuauuacu gaaucaacag aaaauaguaa aaugaugcua     1200

gaauuagacc caccauuugg agauucguau auaguaauag gaguaggcga uaaaaaaaua     1260

acacaucauu ggcauagauc aggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu auuaggagau acagcauggg acuuuggauc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4941
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 1 opt

<400>  4941
gggagaaagc uuaccauguc gcaaaaagua auauauuuag uaaugauucu auuaauagcu       60

ccugcauauu caauaagaug uauaggugua agcaauagag auuuuguaga aggaaugagu      120

ggaggaacgu ggguagaugu aguauuagaa cacggaggau guguaacugu aauggcacaa      180

gauaaaccaa cuguagauau agaauuagua acaacuacag uaucaaauau ggccgaagua      240

agaagcuauu gcuaugaagc uaguauaagu gacauggcau cagacucaag auguccaaca      300

caaggcgaag cauaccuaga uaaacaaagu gauacacaau augucugcaa aaggacgcua      360

guagauagag gauggggaaa uggauguggu cuauuuggaa aagggucauu agucacaugu      420

gcaaaauuug cauguucaaa gaaaaugaca ggaaaaucaa uacagccaga aaauuuagaa      480

uauagaauua uguuaagugu acacggaagc caacacucag gaaugauugu aaacgacaca      540

ggacaugaaa ccgaugaaaa cagagcaaaa guagaaauaa caccuaauuc accaagagca      600

gaagcaacgu uaggaggauu ugguucuuua ggacuagauu gugaaccaag gacaggacua      660

gauuuuagug acuuauauua cuuaacaaug aacaauaaac auuggcuagu ucauaaagaa      720

ugguuucaug auauaccucu accauggcac gccggagcag auacuggaac cccacauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaaagaca aacuguagua      840

guauuagguu cucaagaagg ugcaguacac acagcuuuag caggagcauu agaagcagaa      900

auggauggug caaaaggaag acuaaguagu ggacacuuaa aauguagauu aaaaauggau      960

aaacuaagau uaaaaggagu aucauauuca uuauguacug cagcauucac auucacaaaa     1020

auuccagcag aaacuuuaca uggaacaguu acaguugaag uacaauaugc aggaacagau     1080

ggaccaugca aaguaccagc gcaaauggca guagauaugc aaacauuaac uccaguagga     1140

cgauuaauaa ccgcaaaucc aguaauaaca gaaaguacag aaaacaguaa aaugaugcuu     1200

gaauuagacc caccauuugg agauucauau auuguaauag guguaggaga caagaaaaua     1260

acacaccacu gguacagauc gggaucaacg auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu auuaggagac acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4942
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 1 opt

<400>  4942
gggagaaagc uuaccauguc acaaaaagug auauauuuag uaaugauauu acuaauagca       60

ccagcauaca guauaagaug uauaggagua ucuaauagag auuuuguaga aggaauguca      120

ggaggaacau ggguagaugu aguacuagaa cacggaggau guguaacagu aauggcacaa      180

gacaaaccaa caguagauau agaauuagua acaacaacug uaagcaacau ggcagaaguu      240

agaaguuauu guuaugaagc aaguauaagu gauauggcaa gugauucaag auguccuaca      300

caaggagaag cauacuuaga caaacaaagc gauacacaau auguuugcaa aagaacguua      360

gucgaccgag gaugggggaa cggaugugga cuauuuggaa aaggaagucu aguaacaugu      420

gcuaaauuua cauguagcaa aaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa      480

uacagaauaa uguuaagugu gcauggauca caacauagcg gaaugauagu uaaugauacu      540

gggcacgaaa cugaugaaaa uagagcgaaa guagaaguca caccaaacag uccaagagcg      600

gaagcaacuu uaggaggauu cgguucauua ggacuagauu gcgaaccuag gacuggauua      660

gauuuuucag aucuauauua ucuuacaaug aauaauaaac auugguuagu acauaaagaa      720

ugguuucaug auauaccacu accguggcau gcaggagcag auacaggaac accucauugg      780

aauaacaaag aagcauuagu agaauucaaa gaugcacaug caaaacgaca aacgguagua      840

guacuuggaa gucaagaagg agcaguacac acagcauuag caggagcuuu agaagccgag      900

auggacgggg caaaaggcag acuauuuagc ggacacuuaa aaugcagauu aaaaauggau      960

aaauuaagau uaaagggagu aaguuauuca uuauguacag cugcauuuac uuuuacaaaa     1020

guuccagcag agacauuaca uggaacagua acaguggaag uacaauaugc aggaacagau     1080

gggccaugua aaguuccagc acaaauggca guagauaugc aaacauuaac uccaguagga     1140

agacuuauua cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugaugcuu     1200

gaauuagacc caccauuugg ggauucuuau auagucauag gaguuggaga uaaaaaaaua     1260

acucaucauu ggcauagauc uggcucaacu auaggaaaag cguuugaagc aacgguaaga     1320

ggagcaaaaa gaauggcugu acuaggcgac acagcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4943
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 1 opt

<400>  4943
gggagaaagc uuaccauguc acaaaaaguu auauauuuag uaaugauauu guuaauagca       60

ccggcauaua guauaagaug uauaggagua ucaaauagag auuuugucga aggaaugagu      120

ggaggaacau ggguugaugu aguauuagaa cauggcggau gcguaacugu aauggcacaa      180

gauaaaccaa cuguggauau agaauuagua acaacaacag uaagcaauau ggcagaagua      240

agaaguuauu guuaugaagc aucaauaagu gauauggcaa gugauucucg augcccgaca      300

caaggagaag cauacuuaga uaaacaaagu gauacacaau auguaugcaa gcgaacacuu      360

guagacagag gauggggaaa cggauguggu uuauucggua aaggaaguuu aguaacuugu      420

gcaaaauuua cauguagcaa aaaaaugaca ggaaaaucaa uacaaccuga gaauuuagaa      480

uauagaauua ugcuaagcgu acacggguca caacauagcg gaaugauagu aaaugauaua      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaguaa cuccaaauag uccaagagca      600

gaagcaacau uagggggcuu cgguucauua gguuuagacu gcgaaccacg gacuggacua      660

gacuucucag auuuauauua uuuaacaaug aauaacaaac acuggcuggu acauaaagaa      720

ugguuucacg auauaccuuu accauggcau gcgggcgcag auacaggaac accucauugg      780

aauaacaaag aagcacuagu agaauuuaaa gaugcacaug caaaacgaca aacaguagua      840

guauuaggaa gucaagaagg ggcaguacau acagcacuag cgggagcauu agaggcagaa      900

auggauggag caaaaggaag guuauucagc ggacauuuaa aauguagacu uaaaauggau      960

aaauuaagau uaaaaggagu aagcuauagu uuauguacag cagcauuuac auuuacaaaa     1020

guaccagcag aaacauuaca uggcacagua accguagaag uacaauaugc aggaacagau     1080

ggaccaugca aaguaccagc ucaaauggcu guagauaugc aaacacuaac accuguagga     1140

agacugauua cagcuaaucc agucauaaca gaaaguacgg aaaauagcaa aaugauguua     1200

gaacuagauc caccauuugg ggauagcuac auaguaauag gaguaggaga uaaaaaaaua     1260

acacaucacu ggcauagauc aggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaac gcauggcagu auuaggggac acagcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4944
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 1 opt

<400>  4944
gggagaaagc uuaccaugag ucaaaaaguu auauauuuag uuaugauauu acuaauagca       60

ccagcauauu caauaagaug uauaggagua agcaacagag auuuuguuga aggaaugagc      120

gguggaacau ggguagacgu aguauuagaa cauggaggau guguaacagu aauggcacaa      180

gauaaaccaa caguagacau agaacuagua acaacaacag uuucaaauau ggcagaagua      240

agaaguuauu gcuaugaagc aaguauaagu gacauggcaa gugauaguag auguccaacu      300

caaggugaag cauacuuaga uaaacagagu gacacacaau acguauguaa acgaacucua      360

guagacagag gcuggggaaa uggauguggg cuuuucggaa aaggaucauu aguuacgugu      420

gcaaaauuua caugcaguaa aaaaaugaca ggaaaaucaa uacaaccaga aaaucuggaa      480

uauagaauaa uguuaagcgu acauggaagu caacauucag gaaugauagu aaacgauauc      540

ggacaugaaa cagaugaaaa cagagcaaaa guagaaguaa cuccaaauag cccaagagca      600

gaagcaacau uaggaggauu uggaucauua ggucuagauu gugaaccaag aacaggacua      660

gauuuuagug aucuauacua ucuaacaaug aauaauaaac auugguuagu acacaaagaa      720

ugguuccaug auauaccauu acccuggcau gcaggagcag acacaggaac accacauugg      780

aacaauaaag aagcauuagu agaauuuaaa gacgcacaug caaaaagaca aacuguagua      840

gugcuaggaa gucaagaagg agcuguacac acagcacuag caggugcauu agaggcagag      900

auggauggag caaaaggaag auuauuuagu ggacaucuaa aguguagacu aaaaauggac      960

aaauuaagau uaaaaggagu aaguuauagu cugugcacag cuguaugcac agcagcaaaa     1020

guaccagcag aaacuuuaca uggaacagua acuguagaag uacaauaugc agggacagau     1080

gguccuugua aaguaccagc ucaaauggca guagacaugc aaacgcuaac accaguagga     1140

agacuaauca cagcaaaucc aguuauaacc gaaaguacag aaaauagcaa aaugauguua     1200

gaacucgauc cuccauuugg agauagcuau aucguaauag gaguaggaga caaaaaaaua     1260

acucaucauu ggcauagaag uggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggugcaaaaa gaauggcagu acuaggagac acagcauggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4945
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 1 opt

<400>  4945
gggagaaagc uuaccauguc acaaaaagua auauacuuag uuaugauauu acuaauagca       60

ccggcuuaua guauaagaug cauaggagua agcaauagag auuuuguaga aggaaugagc      120

ggaggaacau ggguagaugu aguauuagaa cacggaggcu gcguaacagu aauggcucaa      180

gauaaaccaa cuguagauau agaacuagua acaacuacag uaucaaauau ggcagaggua      240

agaaguuauu guuaugaagc aucaauaagc gauauggcau cagacaguag auguccaaca      300

caaggugaag cauauuuaga uaaacaaagu gauacacaau auguauguaa aagaacccua      360

guagacaggg gauggggaaa uggcugugga uuauuuggaa aaggaucacu aguuacaugu      420

gcaaaauuca cauguagcaa aaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa      480

uacagaauaa uguuaagugu acacggaagc caacauucag guaugauagu aaaugaugaa      540

aacagagcaa aaguagaagu uacaccaaau ucaccaagag cagaagcaac acuaggagga      600

uuugguucuc uuggauuaga uugugagcca agaacagguc uggauuuuag ugacuuauau      660

uacuuaacaa ugaauaauaa acauugguua guucacaaag aaugguucca ugauauuccc      720

uuaccauggc auucaggagc agauacagaa acaccacauu ggaauaauaa agaagcauua      780

guagaauuua aagaugcaca ugcaaaaaga caaacaguug uuguacuggg aagccaagaa      840

ggggcaguac auacagcauu agcuggagca cuagaagccg aaauggaugg agcgaaaggc      900

agacuaucaa guggacauuu aaaauguaga uuaaaaaugg auaaauuaag auuaaaagga      960

guaagcuacu cauuauguac agcagcauuc acauuuacaa aaguaccagc agaaacauua     1020

caugguacag uaacaguaga aguccaauau gcuggaagag auggaccaug uaaaguacca     1080

gcacaaaugg caguagauau gcaaacauua acaccaguug guagauuaau aacagcuaau     1140

ccagugauaa cugaaucaac agaaaauagu aagaugaugu uagaacuaga uccaccauuc     1200

ggagacaguu auauaguaau aggagucgga gauaaaaaaa uaacgcauca uuggcauaga     1260

agcggaagua uuauaggaaa agcauucgaa gcaacaguaa gaggagcaaa aagaauggcg     1320

guacuaggag auacagcuug ggauuuuggu aguugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  4946
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 1 opt

<400>  4946
gggagaaagc uuaccauguc gcaaaaagua auauaucucg uuaugauagu ucuaauagua       60

ccagcuuaca guauaagcug uauaggagua ucaaauagag acuuagugga aggcaugagc      120

ggagguacau ggguagacgu aguauuagaa cacggaggau gcguaacaga aauggcccaa      180

gauaaaccaa cuguggauau agaauuagua acgaugacag uaagcaauau ggcugaggua      240

agaagcuauu guuacgaagc aaguuuauca gauauggcua gcgcaucaag guguccaaca      300

caaggcgaac caucauuaga uaaacaaagu gauacucaaa guguauguaa aagaacucua      360

ggagauaggg gauggggaaa uggaugcgga auauucggua aaggaagucu aguaacaugc      420

aguaaauuca cauguuguaa aaaaaugccu ggaaaaucaa uucaaccaga aaauuuagaa      480

uacagaauaa ugcuaccagu acauggaagu caacauagcg gaaugauagu uaaugauaua      540

ggacaugaaa cugaugaaaa cagagcaaaa guagaaguaa cuccaaacag cccuagagca      600

gaagcaacac ucggaggauu cggaucacuc ggauuagauu gugaaccaag aacaggacuu      660

gacuucagug aucuuuacua uuuaacgaug aauaacaaac auugguuagu acauaaagaa      720

ugguuccaug auauaccauu accauggcau gcaggagcag auacaggaac accacauugg      780

aacaauaaag aagcguuagu agaauuuaaa gaugcacaug caaaaagaca aacaguagua      840

guauuaggaa gccaagaagg agcuguacac acggcacuag caggggcauu agaagcagaa      900

auggauggag ccaaagguag auuauuuagu ggacauuuaa aauguagauu aaaaauggau      960

aaacuaagac uaaaaggagu aaguuacagc uuauguacag cagcauuuac auucacaaag     1020

guaccagccg aaaccuuaca cggaacaguu acaguagaag uccaaagugc aggcacagau     1080

gguccaugua aaguaccggc acaaauggca guagauaugc aaacuuuaac accuguagga     1140

agguuaauaa cagcaaaucc aguaauaaca gaaagcacag aaaauaguaa aaugaugcua     1200

gaauuagacc caccauuugg agauucauau auaguaauag gagucggaga uaaaaaaaua     1260

acacaucauu ggcacagaag uggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggcgccaaaa gaauggcagu auuaggagau acagcauggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4947
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 1 opt

<400>  4947
gggagaaagc uuaccaugag acaaaaagua auauauuuag uaaugauauu acuaauugca       60

ccugcauauu caauaagaug cauagguguu aguaauagag auuuuguuga aggaauguca      120

ggaggaacuu ggguagacgu aguacuagaa caugguggcu guguaacagu aauggcacaa      180

gauaaaccaa caguagauau agagcuaguu acgacaacag uuagcaacau ggcagaggua      240

agaaguuauu guuaugaagc aaguauauca gacauggcaa gcgauucaag guguccaaca      300

caaggugaag cauacuuaga uaaacaaagu gauacacaau acguauguaa aagaaccuua      360

guugauagag guuggggaaa ugguugugga cuauuuggaa aaggaaguuu aguuacaugc      420

gcaaaauuug cauguaguaa aaaaaugaca ggaaaaucaa uacaaccaga gaauuuagaa      480

uauagaauaa ugcuuagugu acauggaagu caacauagcg gaaugauagu aaaugacaca      540

ggacaugaaa cugaugaaaa uagagcaaaa guagaaauaa caccaaauuc acccagagca      600

gaagcuacau uaggaggauu uggaucauua ggauuagacu gugaaccuag aaccggauua      660

gauuuuucag aucuauauua cuuaacaaug aauaauaaac auugguuagu acauaaagaa      720

ugguuucaug auauaccguu accauggcau gcaggcgcag auacaggaac cccacacugg      780

aauaauaaag aagcacuagu agaauuuaaa gaugcccaug caaaacguca aacuguagua      840

guacuaggau cacaagaagg agcaguacac acugcauuag ccggagcuuu agaagcagaa      900

auggauggag cgaaaggaag acuaucaucu ggacaccuaa aauguagauu aaaaauggau      960

aaauuaagau uaaaaggagu aaguuauagu uuaugcacag cagcauucac auuuacgaaa     1020

auuccagcag aaacauuaca cggaacagua acaguagaag uucaauaugc gggaacagac     1080

ggaccaugua aaguaccagc acaaauggca guugauaugc agacauugac ucccguugga     1140

agacuaauaa cagcaaaucc aguaauaacu gaaucaacag aaaacucaaa aaugaugcua     1200

gaauuagauc caccauuugg agauucauau auaguaauag gaguagguga aaaaaaaaua     1260

acucaucauu ggcacagaag ugggaguaca auaggaaaag cauuugaagc aacuguaaga     1320

ggggcaaaac gaauggcagu acuaggagau acugcauggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4948
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 1 opt

<400>  4948
gggagaaagc uuaccaugag ucaaagagua auauacuuag uaaugauauu auugauagca       60

ccagcauauu caauuagaug uauaggagua aguaauagag auuuuguaga aggaaugagu      120

ggaggaacau ggguagaugu aguauuagaa cauggaggau guguaacugu uauggcacaa      180

gauaaaccaa caguagauau agaauuagua acaacaacag uaucaaauau ggcagaagua      240

agaagcuacu guuacgaagc aucaaucucu gauauggcaa gugauaguag auguccgaca      300

caaggagaag cuuauuuaga uaaacaaagc gauacacaau auguaugcaa aagaacauua      360

guugauagag gauggggaaa uggaugugga uuauuuggaa aagguagucu aguaacaugu      420

gcuaaauuua cauguagcaa aaaaaugaca ggaaaaagua uacaaccaga aaaucuagaa      480

uauaggauaa ugcuaucagu acacggaagu caacacagcg gaaugauugu aaaugacaua      540

ggacacgaaa cagaugaaaa ucgagcaaaa guagaaguaa cuccaaauuc accuagagca      600

gaagcaaccu uaggagguuu ugggucauua ggacuagacu gugaaccaag aacaggauua      660

gauuuuagug acuuauauua cuuaacaaug aauaauaaac auuggcuagu acacaaagaa      720

ugguuucacg auauaccacu accauggcac gcaggagcag auacuggaac accacauugg      780

aauaauaaag aagcauuggu ugaauuuaaa gaugcacaug cuaaaagaca aaccguagua      840

guguuaggca gccaagaagg agcaguacau acagcuuuag caggagcauu agaagcagaa      900

auggauggag caaaagguag auuauuuuca ggacaucuga aauguagaau aaaaauggau      960

aaauuaagau uaaaaggagu auccuauagu uuauguacag cagcauucac guuuaguaaa     1020

guaccagcag aaacguuaca ugguacagua acaguagaag uacaauaugc agguagggac     1080

ggaccgugua aaguaccagc acaaauggcc guagauaugc aaacauuaac accaguagga     1140

cgauuaauaa cagcaaaccc aauaauaaca gaaucaacug aaaauaguag aaugauguua     1200

gaauuagauc caccauuugg agauucauau auaguuauag guguaggaga uaaaaaaaua     1260

acccaucauu ggcauagaag uggcucaaca auagguaagg cauuugaagc gacaguaaga     1320

ggagcgaaaa gaauggcugu gcuaggagau acugcauggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4949
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  4949
gggagaaagc uuaccaugcg uauaaucaac gcacgaaaag agaagaaaag gcgcggcgcc       60

gacacuucag ucgguaucgu cggccuccuc cucacaaccg ccauggccgc agaagucacc      120

cgccgaggaa gugcauacua cauguaccua gaccgcaaug acgccggaga agcaaucagc      180

uuccccacca cacucgguau gaacaaaugc uacauacaaa uaauggaccu cggucacaug      240

ugcgacgcca cgaugagcua cgaaugcccc augcucgacg aaggggucga accagacgac      300

guugacugcu ggugcaacac aaccucuacu ugggucgucu acgguaccug ccaccacaag      360

aaaggcgaag cgcgacgauc ccgcagggcc gucacccugc cgagccauuc aacccgaaaa      420

cuacaaaccc gaagccaaac cuggcucgaa ucucgcgaau acaccaaaca cuugauccgc      480

gucgaaaacu ggauuuucag aaacccaggc uucgcacucg cggcagccgc aauagccugg      540

cuccucggcu caucaacauc ccagaaaguc aucuaccucg uaaugauucu acucaucgcc      600

ccagcauacu caauacggug cauaggagug uccaaccgcg acuucgucga gggaauguca      660

ggcggcacau ggguagacau aguacucgaa cacggaggau gcgucaccgu aauggcccaa      720

gacaaaccaa ccguugacau agaacucgua accacaaccg uaagcaacau ggcagaaguc      780

cgcucauacu gcuacgaggc auccaucagc gacauggccu ccgacagccg augccccacc      840

caaggagagg ccuaccucga uaaacaaagc gacacccaau acgucugcaa aagaacccuc      900

guagauagag guuggggcaa cggcugcggu cuauucggca aaggaagccu aguaacaugc      960

gccaaauucg ccugcuccaa gaaaaugacc ggcaaaucca uccaaccgga gaaccuugaa     1020

uauagaauca ugcuguccgu acacggaucc caacacuccg gaaugauagu caacgacaca     1080

ggccacgaga ccgacgagaa ccgagcaaaa guagagauca cacccaacuc gccaagagcc     1140

gaagccaccc uagguggcuu cggaucacuu ggccucgacu gcgaaccccg gacagggcuc     1200

gacuucucag accucuacua ccucaccaug aacaacaaac acuggcuugu acacaaagaa     1260

ugguuucacg acaucccacu cccauggcau gcaggcgcag acaccggcac accacacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg caaaacgcca aacaguggua     1380

guccuggguu cccaagaagg cgccguccac acagcccucg ccggcgcccu cgaagcagaa     1440

auggacggcg caaaaggacg ccuuagcucc ggccaccuaa aaugcagacu caaaauggac     1500

aaacuccgcc uuaaaggcgu aucauacagc cucugcacag cugcauucac cuucaccaaa     1560

auacccgccg aaacccucca cggaacaguc accgucgaag uacaauacgc aggaacggau     1620

ggcccuugca aaguaccagc ccagauggcc gucgacaugc agacccuaac accagucgga     1680

agacucauaa ccgccaaucc agucauaaca gaguccaccg aaaacuccaa aaugaugcuc     1740

gaacuagacc cuccauuugg cgacuccuac auagucauag gcguaggaga aaagaaaaua     1800

acacaccacu ggcaccgcuc cgguuccacc auuggaaaag cauucgaagc gacaguacgc     1860

ggggcaaaac guauggcggu gcucggcgac accgccuggg acuuuggguc cgucggcggc     1920

gcccucaauu cacucggcaa aggaauacau caaaucuucg gagcggccuu caaaucccua     1980

uucggcggca ugagcugguu cucccaaaua cugaucggca cacuacucau guggcuaggc     2040

cucaacacca aaaacggcuc aaucucccuc augugccugg cacucggcgg cguacucauc     2100

uuccuaucca cagcagugag ugccgacgug ggaugcuccg uagacuucuc gaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4950
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 1 opt

<400>  4950
gggagaaagc uuaccaugcg caucaucaac gcccgaaaag aaaagaaacg acgaggagcg       60

gacacgucag ucggcaucgu aggauuacug cucaccacag caauggccgc cgaagucacc      120

cgcaggggca gcgcauauua cauguaccuu gaccgaaacg acgcggggga agcaauaucg      180

uuccccacca cgcuaggcau gaacaaaugc uacauccaaa uaauggaccu aggccauacc      240

ugcgacgcca caaugucaua cgaaugccca augcuagacg aaggcguaga acccgacgac      300

guagacugcu ggugcaacac aacaagcaca ugggucgucu acgggacaug ccaccacaag      360

aaaggcgaag cucgcagaag cagacgcgcc gucacacugc caagccacuc cacccgaaaa      420

cuacaaaccc gcagccaaac cuggcucgaa agccgcgaau acacaaaaca ccucauacga      480

gucgaaaacu ggaucuuccg aaaccccggc uucgcccugg cagcagcagc cauagccugg      540

cuccuaggcu caagcacaag ccaaaaagua auauaccuag ucaugauccu acuaaucgcg      600

ccagcguacu caauccgaug caucgguguc agcaaccgag acuucgucga aggcauguca      660

gguggcaccu gggucgaugu cguccuggaa cacggcgguu gcgucacggu cauggcccag      720

gauaaaccua ccguagacau agaacugguc accacgaccg uaagcaacau ggcagaagug      780

aggagcuauu gcuacgaagc aucaauaucg gacauggccu cugauucgcg augcccgacc      840

caaggcgagg cauacuugga uaaacaauca gauacccaau acguauguaa acgaacgcua      900

guagaccgag ggugggggaa cgguugcgga cucuuuggaa aaggcucccu aguaaccugc      960

gccaaauucg cguguucaaa gaaaaugaca ggcaaaucaa uacagccaga aaaccucgaa     1020

uaccggauaa ugcucuccgu ccacgggucc caacacagcg gcaugauagu caacgacaca     1080

ggccacgaaa cagacgaaaa uagggccaaa gucgaaauaa caccaaacuc accacgagcg     1140

gaagccacgc ucggcggauu cggaucccua ggccucgacu gugaaccccg gaccggccug     1200

gacuuuagcg accuauacua ccucacaaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuucacg acaucccccu gccauggcac gcgggcgcag acacuggcac accacacugg     1320

aacaacaaag aagcucuugu cgaguucaaa gacgcacacg ccaaaagaca aacagucgug     1380

guacucggau cccaagaagg agccguacac accgcccucg ccggcgcccu cgaagccgag     1440

auggacggug ccaaaggacg ccucagcagc ggacaccuaa aaugcagacu aaaaauggac     1500

aagcucagac ucaagggcgu auccuacucc cucugcaccg ccgccuucac cuucacaaaa     1560

auccccgccg aaacccuaca cgggaccguc accgucgaag uacaguacgc cggcacagau     1620

ggccccugua aaguaccagc ccaaauggcc gucgacaugc aaacccugac acccgucgga     1680

cgccuaauaa cggccaaccc cgucaucacc gaaucaaccg aaaacuccaa aaugaugcuc     1740

gaacuagacc cacccuucgg cgacagcuac aucgucaucg gggucggaga aaagaaaaua     1800

acccaccacu ggcaccgauc cgggucaacc aucggcaaag cauucgaagc caccguccgc     1860

ggcgcaaaac gcauggcggu ccugggagac acagcuuggg acuucggauc cgucggcggc     1920

gcccuaaacu cacucggaaa aggcauucac caaaucuucg gcgccgccuu uaaaagccuc     1980

uucggcggaa ugucgugguu cucccagauc cuuauaggaa cccuacuaau guggcucggc     2040

uugaacgcca aaaacggaag cauaagucug augugccucg cacucggagg cguccuaauc     2100

uuccuaagca ccgcaguaag cgccgacguu ggauguagug uagacuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4951
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 1 opt

<400>  4951
gggagaaagc uuaccaugag aauaaucaac gcccgaaaag aacgcaaacg cagaggagcc       60

gacacaagca uaggcauuau cggccuacuu cucaccaccg caauggcugc agaaaucacc      120

agacgaggaa gcgccuauua cauguaucuc gacagaucag augcaggaaa ggcaaucucc      180

uucgccacca cacucggagu aaacaaaugc cacgugcaaa ucauggaccu cggacacaug      240

ugcgacgcga caaugucaua cgagugcccc augcuagacg agggagucga gccagacgac      300

guagacugcu ggugcaacac aaccucaacc ugggucgucu acggaacaug ccaccacaaa      360

aagggggaag cacgccgaag ccgcagagcg gucacacuac caagucacuc cacacgcaag      420

cuccaaaccc ggagccagac cuggcuagaa ucccgggaau acaccaaaca ccuaauaaaa      480

guagagaacu ggauauuucg gaacccagga uucgcgcucg ucgccguagc caucgcaugg      540

cuacuaggcu ccagcacauc acaaaaaguc auauaccuug uaaugauccu ccucaucgcc      600

cccgcauacu ccauccgcug cauaggaguc agcaaucgcg acuucgucga aggcauguca      660

ggaggaaccu ggguagaugu aguacucgag cauggcggcu gcgucacagu cauggcacaa      720

gacaaaccaa ccguagacau cgaacucguc acaaccaccg ugagcaacau ggcugaaguc      780

cggucauacu gcuacgaggc aucaauaucc gacauggccu ccgacagccg cugcccaacc      840

caaggcgaag ccuaccucga caaacagagc gacacacaau acgucugcaa acgaacccuc      900

guagacagag gcugggggaa cggaugcgga cuauucggca aaggaucccu cguaaccugc      960

gccaaguuca cgugcagcaa gaaaaugacc ggcaaaucca uucaacccga aaaccuagag     1020

uaccgcauca ugcuaucagu ccacggcagu caacacucag ggaugaucgg cuacgaaacc     1080

gacgaagacc gcgccaaagu ggaagucaca ccaaacucac cacgcgccga agcaacacua     1140

ggcggguucg gaucccuggg acucgacugc gagccccgca cgggccuaga cuuuagcgac     1200

cuuuacuauc uaaccaugaa caacaaacac uggcuaguac acaaagagug guuccacgac     1260

aucccacuac cguggcacgc aggggcagau accggcacac cgcacuggaa caauaaagaa     1320

gcccuaguag aguucaaaga cgcacacgcc aaacgacaaa ccguaguggu cuuaggaucc     1380

caggaaggag ccguacacac cgcccucgcc ggagcccuag aagccgaaau ggacggcgcc     1440

aaaggccgcc uauuuucggg ccaccucaaa ugccgccuga aaauggacaa acuacgccua     1500

aaagggguaa guuacucccu cugcaccgcc gcauucacau ucacuaaagu cccagccgaa     1560

acccuccacg gcacaguaac aguagaaguc caauacgccg gaaccgacgg acccugcaaa     1620

auccccguac aaauggcagu agauaugcaa acccuaaccc ccgucggccg ccuaauaaca     1680

gccaaucccg uaauuaccga auccacagag aacagcaaga ugaugcucga acuugaccca     1740

cccuucggcg acucguacau agucaucgga gucggcgaca agaaaauuac acaccacugg     1800

caccggucag gaucgacaau cggcaaagcg uucgaggcaa ccguacgagg agccaaaaga     1860

auggccguac uaggugacac cgcgugggac uucggcuccg uagggggcgu auucaacucc     1920

cuaggaaaag gaauccacca gaucuucggc gccgcauuca agucacuauu cggggguaug     1980

agcugguuca gccaaauccu aaucggcacc cuacuggugu ggcucggucu caacaccaaa     2040

aacggaucaa ucucccucac augccuagca uuagggggag ucaugaucuu ccucagcaca     2100

gcagucucag ccgacguggg augcagcgua gacuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4952
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 1 opt

<400>  4952
gggagaaagc uuaccaugag aaucaucaac gccaggaaag aaaagaaaag acgaggagca       60

gauaccagug uaggcauagu aggcuuacuc cucacuacag caauggcagc cgaaguaaca      120

agacggggcu ccgcguacua cauguaccuc gaccgaaacg acgcaggaga ggcgaucagc      180

uuccccacga cgcuagggau gaacaaaugc uacauacaaa ucauggaccu cggccacaug      240

ugcgacgcaa caaugagcua cgaaugcccc augcuggaug aaggagugga gcccgacgac      300

guagacugcu ggugcaauac cacaaguacc uggguggugu acggaacaug ccaccacaag      360

aaaggcgaag cccgacgauc gcgccgagcc guaacucugc cgucccauuc gaccagaaaa      420

cuccaaacaa gaucacagac auggcuagag ucacgggaau auacaaaaca ccucauccgc      480

guagaaaacu ggauauuccg aaacccgggc uucgcccucg cagcggcagc aaucgccugg      540

cugcugggga guucgaccag ccaaaaaguc auauaccuag uaaugauccu acucaucgca      600

ccggcuuaca gcauacgcug cauaggaguc ucaaaccgag acuucgucga aggaaugagc      660

ggcggcaccu ggguagacgu aguacucgag cacggcgggu gcguaaccgu cauggcacaa      720

gacaaaccaa caguagacau ugagcuaguc acgacaacag uauccaacau ggcugaagua      780

agaaguuacu guuacgaagc cagcaucagc gacauggcaa gcgacucucg cugccccacc      840

caaggagaag ccuaccucga caaacaguca gacacccagu acguauguaa acgaacgcua      900

guagacagag gcuggggcaa cggauguggc cuuuuuggaa aaggauccuu agucacaugc      960

gccaaauucg ccugcagcaa gaaaaugaca gggaaaucaa uccagccaga aaaccuagaa     1020

uaccggauca ugcucagcgu ccacggcucc cagcacucgg gaaugaucgu aaacgacaca     1080

ggccacgaaa cagacgaaaa ucgcgccaaa guagaaauaa ccccgaacag cccccgcgca     1140

gaagccacuc uaggagguuu cggaagccuc ggucuagacu gcgagccccg caccgggcua     1200

gacuucagug accuauacua ccugacaaug aacaacaaac acuggcuagu ccacaaagaa     1260

ugguuccaug acaucccacu accguggcac gcaggcgcag auaccggcac cccacacugg     1320

aauaacaaag aagcccucgu cgaguucaaa gacgcgcacg ccaaacgcca aacugugguc     1380

guacucggga gucaggaagg cgcaguacac accgcacucg caggcgcccu agaagcagaa     1440

auggacggcg caaaaggacg ccuauccagc ggacaccuca aguguagacu aaaaauggac     1500

aaacuccgac ucaaaggugu aagcuacucc cuaugcacgg cagcauucac cuucacgaaa     1560

auaccagcgg aaacacucca cggaacagua acaguagaag uccaauacgc agggacugac     1620

gggccaugca aaguacccgc gcaaauggca guagacaugc aaacacucac accggucgga     1680

cgacuaauaa cagcaaaccc gguaaucacc gaaagcaccg aaaacagcaa aaugaugcuc     1740

gaacuagacc cacccuucgg agacucauac aucgucaucg gcguaggaga aaagaaaauc     1800

acgcaccacu ggcacagauc aggaucgacu auaggcaaag ccuucgaagc aacagucaga     1860

ggggccaaac gcauggcagu acugggggac acagccuggg acuucggguc cgucggcggg     1920

gcucuaaaca gccuaggcaa agggauacac caaaucuucg gcgcagcauu caaaucacuc     1980

uucggcggca ugagcugguu cagccaaauc cuaaucggaa cacuacucau guggcuaggg     2040

uuaaacacca aaaacggcag caucagccuc augugcuuag cccuaggcgg gguacucaua     2100

uuucuaagca cagcaguaag cgccgacgua ggaugcagcg uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4953
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  4953
gggagaaagc uuaccaugac guccguaggc aucguggggc uccugcuaac gacggccaug       60

gcagcagagg uaacccgccg aggcucagca uacuacaugu accuagaccg aaacgacgcg      120

ggagaagcca ucagcuuccc caccacccuc ggaaugaaca aaugcuacau ccaaauaaug      180

gaccucggcc acaugugcga cgcgacaaug ucauacgaau gcccaauguu agacgaaggg      240

gucgaacccg acgacgucga cugcuggugc aacaccacaa gcacaugggu aguauacggu      300

accugccacc acaagaaagg cgaagccaga cgcagccgca gagccguaac ccucccgucc      360

cacagcacac ggaaacucca gacacggagu caaacauggc uggaaucgcg cgaauacacc      420

aaacaccuua uaagaguaga aaacuggauc uuccgaaacc cgggcuucgc gcucgccgca      480

gccgccaucg cauggcuccu gggguccagc accagccaaa aagucauaua ccucgucaug      540

auccuccuca uagcccccgc cuacuccauc cgcugcaucg guguguccaa ccgagacuuc      600

guagaaggaa ugucgggagg aacaugggua gacauagucc ucgaacacgg cgggugcguc      660

accgucaugg cgcaagacaa accaacagua gacauagagc uaguaacaac aacagucagc      720

aacauggcug aaguccgauc cuacugcuac gaagcaucaa ucagcgacau ggcaucugac      780

agucgaugcc ccacacaagg ugaagccuac cucgacaaac agucagacac acaauacgua      840

ugcaaacgca cacucguaga cagaggcugg ggcaacggau gcggacuguu cggcaaaggc      900

ucacugguaa caugcgccaa auucgcaugc agcaagaaaa ugacaggcaa aagcauccaa      960

ccagaaaacc uagaauaccg caucaugcua agcguccacg gaagccaaca cagcggcaug     1020

auagucaacg acaccgggca cgaaacagac gaaaaccgag ccaaagucga aaucacgcca     1080

aacagccccc gcgccgaagc aacacuaggc ggcuucggaa gccucggccu agacugcgaa     1140

ccacgcacag gacuagacuu cagcgaccua uacuaccuca cuaugaacaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacaua ccccucccuu ggcacgccgg cgccgacacc     1260

gguacacccc acuggaacaa caaagaagcc cucguagagu ucaaggacgc ucacgccaaa     1320

cgacaaaccg ucgucguucu cggaucccaa gaaggcgcag uacacacagc acuagccggc     1380

gcccuagaag cugaaaugga cggagccaaa ggacgccuau caucaggaca uuuaaagugc     1440

cgccucaaaa uggacaaacu ccgacucaaa ggagucuccu acagccugug cacagcagcc     1500

uucacauuca caaaaauacc cgcagagacc cugcacggaa ccguaaccgu agagguccaa     1560

uaugccggaa ccgacggccc cugcaaaguc ccagcccaaa uggcagucga caugcaaaca     1620

cucacccccg uaggaagacu aaucaccgca aacccaguaa ucaccgaaag uaccgaaaac     1680

agcaaaauga ugcuagaacu cgauccccca uucggcgaca gcuacauagu caucgggguc     1740

ggcgaaaaga aaaucaccca ccacuggcac cgcucaggau caacaauagg aaaagcauuc     1800

gaggcaaccg uacgcggcgc caaacgcaug gccguccuag gagacacagc augggacuuu     1860

ggcuccguag gaggcgcgcu caacagccuc ggaaaaggaa uacaccaaau cuucggggcc     1920

gccuucaaau cccuauuugg aggaaugucg ugguucucgc aaauccucau aggcacacuc     1980

cugauguggc ucgggcuaaa caccaaaaac ggcuccauca gucuaaugug ucucgcccuc     2040

ggaggagucc uaaucuuccu aagcacagcu guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4954
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 1 opt

<400>  4954
gggagaaagc uuaccaugac cagcgucgga auuguaggac ugcuccuaac gacagcaaug       60

gcagccgaag uaaccagaag aggcuccgca uauuauaugu accucgaccg gaacgacgcc      120

ggagaagcca ucuccuuccc aaccaccuua gguaugaaca aaugcuacau ccaaaucaug      180

gaccucggac acacgugcga cgcaaccaug uccuacgaau gcccaaugcu agacgaaggc      240

guagaaccag acgacguaga cugcuggugc aacaccaccu cgacuugggu aguauacgga      300

accugccacc acaaaaaggg cgaagcacgc cgaucacgca gagccgucac ccuccccuca      360

cacagcaccc gaaaacugca aacacggucc caaaccuggc ucgaaagccg agaauauacu      420

aaacaccuca uaagaguaga gaacuggauu uucagaaacc cgggcuucgc ccucgccgcg      480

gcggcaauag cauggcuccu aggcuccucc acaucgcaaa aagugauaua ucucgucaug      540

auacuacuca ucgccccagc auacuccaua cgaugcaucg gcguauccaa uagagacuuc      600

gucgaaggaa ugagcggagg cacgugggug gacguagucc ucgaacacgg cggcugcgua      660

accgucaugg cacaagauaa accaacggua gacaucgaac ucgucacaac caccguauca      720

aacauggcug aaguccgauc cuacugcuac gaagccucca ucagcgacau ggcauccgac      780

ucacgcugcc ccacccaagg cgaggccuac cuggacaaac aaucagacac ccaauacguc      840

uguaagcgca cccuggugga ccggggcugg ggcaacgggu gcggacucuu cggcaaaggc      900

agccucguaa caugcgcaaa auucgccugc agcaagaaaa ugacaggaaa aagcauccaa      960

cccgaaaacc ucgaauaccg aauaaugcua agcguacacg gaucccaaca cuccgguaug     1020

auaguaaacg acacaggcca cgagaccgac gaaaaccgcg ccaaagucga aauuaccccc     1080

aauucgcccc gagcagaagc uacccuagga ggguuuggcu cccuaggacu ggacugcgaa     1140

ccacgcaccg gacuagacuu cuccgaccua uacuaccuca ccaugaacaa caaacauugg     1200

cuaguacaua aggaaugguu ccacgacauc ccacuacccu ggcacgccgg agccgacaca     1260

ggcacacccc acuggaacaa caaagaggca cucguagagu ucaaagacgc ccacgccaaa     1320

agacaaaccg ucgucguucu cggcucacaa gaaggcgccg uacacacggc ccucgcuggu     1380

gcccuggaag ccgaaaugga cggagccaaa ggacgccucu ccagcggcca ccuaaaaugc     1440

cgccuaaaaa uggacaaacu ccgccucaaa ggcguauccu acucacucug caccgccgcc     1500

uucaccuuca ccaaaauacc cgcagaaacg cuccacggca caguaaccgu cgaaguacag     1560

uacgcuggaa cggauggucc cugcaaagua cccgcccaaa uggcaguaga caugcaaacc     1620

cucacccccg uaggccgccu caucaccgcc aacccaguaa ucacagaauc aaccgaaaac     1680

ucgaaaauga uguuagaacu cgacccaccc uucggagacu cauacaucgu caucggcguc     1740

ggcgagaaaa agaucacgca ccacuggcac cgcagcggcu caacaaucgg aaaggccuuc     1800

gaagcaaccg uaagaggagc caaacgcaug gccguacucg gcgacaccgc augggacuuc     1860

ggcuccgucg gcggagcacu aaacucccuc ggcaaaggaa uccaccaaau cuuuggcgca     1920

gcuuuuaaau cccucuucgg cggcauguca ugguucucuc aaauccucau cggcacacuc     1980

cuaauguggc ucggguuaaa cgcuaaaaac ggcuccauca gccucaugug ccuagcacuc     2040

ggcggaguac uaauauuccu aucaaccgca guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4955
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 1 opt

<400>  4955
gggagaaagc uuaccaugac auccauaggc aucauaggau uacuacuaac caccgccaug       60

gccgccgaaa uaacacgccg gggauccgcu uacuauaugu accucgaccg cagugacgcc      120

ggcaaagcua ucagcuucgc aaccacucua ggaguaaaua aaugccacgu ucaaaucaug      180

gaccuaggcc acaugugcga cgccacaaug uccuacgaau gccccaugcu agacgaaggu      240

guagagccgg acgacgucga cugcuggugc aauaccacca gcaccugggu cgucuacggc      300

acgugccacc acaagaaagg agaagcacgu cguucccgac gcgccguuac ucugccaagc      360

cacuccaccc guaaacucca gaccagauca caaacauggc uagaguccag agaauacacc      420

aaacaccuca ucaaagucga gaacuggaua uuccgaaacc ccggcuucgc ccucgucgcc      480

gucgcaaucg ccuggcuacu cggauccagc acuucacaga aagucaucua ccucgucaug      540

auccuacuca ucgccccggc uuacagcauc cgaugcaucg gagugagcaa ccgugacuuc      600

gucgaaggca ugucuggcgg uaccugggua gacgucgucc uagaacacgg aggcugcguc      660

accguaaugg cccaagacaa acccacuguc gacauagaac ucgucacaac aaccgucagc      720

aauauggcag aaguccgcuc cuacugcuac gaggccucca uaagcgacau ggcaucagac      780

ucccgcugcc caacccaagg agaagccuac cuggacaaac aauccgacac gcaauacguc      840

ugcaaaagaa ccuuagucga ccgcggaugg ggaaacgggu gcggccuauu cggcaaaggc      900

ucgcucguaa ccugcgccaa guucacaugc agcaaaaaga ugacgggcaa aagcauacaa      960

ccggaaaacc ucgaauacag aauaaugcuc ucgguccacg gcucccaaca cagcggaaug     1020

auuggauacg aaacugacga ggacagagcc aaagucgagg uaaccccaaa cuccccgcgc     1080

gcugaagcca cccuaggagg cuucggaagc cugggccuug acugcgaacc ccgaacagga     1140

cuggacuucu cagaccugua cuacuugacc augaacaaca aacacuggcu gguccauaaa     1200

gaaugguucc acgacauccc ccuuccaugg caugcaggcg cagacaccgg cacaccccac     1260

uggaacaaca aagaagcccu cgucgaguuc aaggacgcac acgcaaaacg ucaaaccguu     1320

gucguccuag gcucacaaga aggagcaguu cacaccgccc ucgcgggcgc ccuagaggca     1380

gaaauggacg gagccaaagg gcgccucuuc agcggccacc uaaaaugccg ccuaaaaaug     1440

gacaaacucc gccugaaagg aguaucguac ucccucugca ccgcagcauu caccuucacc     1500

aaaguacccg cggagacccu acacggaaca guaaccguug agguccaaua ugccggaacc     1560

gacggcccau gcaagauccc cguacagaug gccguagaca ugcaaacacu gacaccaguc     1620

ggacgccuaa ucaccgcaaa ccccgugauc acagagucca ccgaaaacag caaaaugaug     1680

cucgagcucg acccaccuuu cggcgacagc uacaucguca uaggagucgg agacaagaaa     1740

auaacccacc acuggcaccg aagcgggucc acgaucggca aagccuucga agcgacaguu     1800

agaggagcca aacguauggc cguacuuggc gacaccgccu gggacuucgg aagugucggc     1860

ggcgucuuca acucacucgg caaaggaauc caccaaaucu ucggagccgc guucaaauca     1920

cuauucggag gaauguccug guucucccaa auccuuauag gaacacuccu gguauggcua     1980

ggucuaaaca ccaaaaacgg cagcauaucc cuaaccugcc ucgcgcucgg cggugucaug     2040

auauuccucu cuaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4956
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 1 opt

<400>  4956
gggagaaagc uuaccaugac caguguaggc aucgucgggc uccuacucac cacggccaug       60

gcggcagagg ucacccgacg uggauccgca uauuacaugu accucgaccg aaacgacgcc      120

ggggaagcga uauccuuccc gaccacccuc ggaaugaaca aaugcuacau acaaaucaug      180

gaccuaggac acaugugcga cgcaacaaug agcuacgaau gcccaaugcu cgacgaaggc      240

guggaaccag acgacgucga cugcuggugc aacaccaccu ccacaugggu aguauacggc      300

acaugccauc acaagaaagg cgaagcccga cgguccagac gcgcugugac acucccaagc      360

cacagcacaa gaaaacucca aacacgcucc cagaccuggc ucgaaagccg ggaauauaca      420

aaacaccuca uacgcgucga aaacuggaua uuccgcaacc cgggguucgc acucgcggcc      480

gccgccaucg cauggcuccu uggcagcagc acuucacaaa aagugauaua ccucguaaug      540

auccuccuga ucgcgcccgc cuacuccauc cgcugcauag gcgucagcaa ccgcgacuuc      600

guggaaggaa uguccggcgg cacguggguc gacgucguac ucgaacacgg aggcugcguc      660

acaguaaugg cccaagacaa accaacgguc gacaucgaac uuguaaccac cacaguaagc      720

aacauggccg aaguccgcuc auacugcuac gaggcaagca uauccgacau ggcaucagau      780

ucacgcugcc ccacccaagg agaagccuac cuggacaaac aaucagacac acaauacgua      840

ugcaaacgga cccucguaga ccgcggcugg ggcaacggcu gcggacucuu uggcaaaggu      900

ucgcucguaa ccugcgccaa auucgccugc uccaaaaaga ugaccggaaa gucaauccaa      960

cccgagaacc ucgaauaccg aaucaugcuc ucgguccacg ggucccaaca caguggcaug     1020

aucgucaacg acacgggaca cgaaacagac gagaacagag ccaaaguaga aaucaccccc     1080

aacuccccaa gagcggaagc aacacucggc ggcuucggca gccuuggccu agacugcgaa     1140

ccacgaaccg gacuggacuu cagugaccuc uauuaccuaa caaugaacaa uaaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccgcuacccu ggcacgcggg ugccgacacc     1260

ggaaccccac acuggaauaa caaggaagcc cuagucgaau uuaaagacgc acacgccaag     1320

agacaaacag ucguaguacu aggcucacaa gaaggcgcag uacacacagc ccuagcagga     1380

gcgcucgaag ccgaaaugga cggcgcgaaa ggccgacuga gcagcggcca ccuaaaaugc     1440

cgacuaaaaa uggacaaacu acgccuuaaa ggagugucau acagccucug cacagcggca     1500

uucaccuuca ccaagauacc ugcagaaaca cuucacggca ccguaaccgu cgaaguacaa     1560

uaugccggaa cagacggccc augcaaggua cccgcgcaaa uggccgucga caugcaaacc     1620

cuaacccccg uaggacgacu aaucacagca aaccccguca ucaccgaauc gacagaaaac     1680

ucaaaaauga ugcuagaacu agauccccca uucggcgacu cauacauagu cauaggcguc     1740

ggcgaaaaga aaaucacaca ccauuggcac cgauccggaa gcacaauagg aaaagcauuc     1800

gaggcaacag uaagaggcgc caaacgcaug gcaguccucg gcgacacagc augggacuuc     1860

ggcagcguag gaggagcacu aaacucccug ggaaaaggaa uacaccaaau auucggcgcc     1920

gcauucaaaa gccucuucgg agggaugagc ugguucuccc aaauccuaau cgggacccuc     1980

cugauguggc uaggccuaaa caccaaaaac gggagcauca gccucaugug ccuagcacua     2040

ggcggcgucc uaauauuccu aucaaccgca guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4957
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 1 opt

<400>  4957
gggagaaagc uuaccauguc acaaaaaguc auauaccugg ucaugauccu acucaucgcc       60

ccagccuaca gcauacgcug cauaggagua ucgaaccgag acuuugucga gggcaugagc      120

ggaggcacau gggucgacau cguccucgaa cacggcgggu gcgugacagu aauggcacaa      180

gacaaaccga caguggacau agagcuaguc acaaccacgg ucucgaacau ggcagaagua      240

aggagcuacu gcuacgaagc cagcauaagc gacauggcau cagacucaag gugcccaaca      300

caaggcgagg cuuaccucga caaacaaagc gacacccaau acgugugcaa acgcacccua      360

guagaccgcg guuggggcaa cggaugcggc cuguucggca aagggagccu agucacaugc      420

gcgaaauucg ccugcagcaa gaaaaugacu ggaaaaagca uccagccgga aaaucucgaa      480

uaccgcauaa ugcucagcgu gcacgggagc caacacucag gaaugaucgu gaacgacacc      540

ggacacgaaa cagacgaaaa cagagcgaaa guagaaauaa ccccaaacuc accaagagcc      600

gaagccacac uaggaggauu cggcucgcua ggccuugacu gcgaaccccg aaccgggcua      660

gacuucucgg accucuauua ccucaccaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccgcu acccuggcac gcaggcgcag acacaggcac accccacugg      780

aacaacaaag aggcccucgu agaauuuaaa gacgcccacg caaaacgcca aacagugguc      840

guacuaggga gccaagaagg ggcaguucac acggcccuag ccggagccuu agaggcggaa      900

auggacggag cgaaaggacg ccucucgagc ggccaucuaa aaugucgccu gaaaauggac      960

aaacucaggc ucaaaggcgu auccuacagc cuauguaccg ccgcguucac auucacaaaa     1020

auacccgcag aaacgcuaca cggcacagua acagucgagg uacaauacgc aggcacagac     1080

gggcccugca aaguacccgc gcaaauggcg guagacaugc aaacccuaac acccgucggc     1140

agacuaauaa ccgcaaaccc aguaaucaca gagagcacag agaacagcaa aaugaugcuc     1200

gagcucgacc caccguucgg agacucauac auagucauag gcgugggcga aaagaaaaua     1260

acgcaccacu ggcaccgcuc cggaucaacc auaggcaaag ccuucgaagc caccguacga     1320

ggcgccaaaa gaauggccgu gcucggagac acagccuggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4958
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 1 opt

<400>  4958
gggagaaagc uuaccauguc ccaaaaaguc auauaccuag uaaugauacu acucauagcc       60

ccagcauaca gcauccgcug caucggcgua ucaaacaggg acuucguaga aggcaugagc      120

ggaggaacau ggguagacgu aguccuagaa cacggcggau gcgugaccgu aauggcacaa      180

gacaaaccga ccguagacau agagcuaguc accacaaccg ucuccaacau ggcagaaguc      240

agaagcuacu gcuacgaagc aagcaucagc gacauggcca gcgacagccg gugcccgacc      300

caaggggaag ccuaccuaga caaacagagc gacacacaau acgugugcaa acgcacacuc      360

guagaccgcg gauggggcaa cggcugcgga cuguucggca aaggaagucu agucacgugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggcaaaucga uccaacccga aaaccucgaa      480

uaccgcauca ugcuaagcgu gcacggcagu cagcacucgg guaugaucgu gaacgacaca      540

ggacacgaaa cagacgaaaa ucgcgcaaaa guggaaauaa ccccaaacuc ccccagagcc      600

gaggccaccc ucggaggcuu cggcucgcuc ggacucgacu gcgaaccccg gaccggccuc      660

gacuucuccg accuguacua ucuaacgaug aacaauaaac acuggcucgu acacaaagaa      720

ugguuccacg acauacccuu acccuggcac gccggcgcag acacaggaac gccacacugg      780

aacaacaaag aagcccucgu ggaguucaaa gacgcacacg ccaagcgcca aaccgucgua      840

guacuaggau cccaagaagg cgccguacac acggcccuag ccggagcccu ggaagcggaa      900

auggacggag caaaaggcag acucagcagc ggacaccuga aaugccgacu gaagauggac      960

aaacuaagac ucaaaggcgu gagcuacagc cucugcacag ccgcauucac guucacaaag     1020

auacccgcag aaacacuaca cggcacagua acagucgaag uccaauacgc cggcacggac     1080

gggcccugca aaguacccgc acaaauggca guggacaugc aaacgcucac acccguggga     1140

agacuaauaa cagcaaaccc agucauaaca gaaagcacag aaaacagcaa aaugaugcua     1200

gaacucgacc cacccuucgg cgacucguac auagugauag gcguaggaga aaagaaaauu     1260

acacaucacu ggcaccgcuc aggcagcaca auaggcaaag ccuucgaagc aacgguccgc     1320

ggagcaaaac gcauggccgu gcucggagac accgccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4959
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 1 opt

<400>  4959
gggagaaagc uuaccauguc gcaaaaagua aucuaccuag ucaugauccu acuaaucgcc       60

cccgccuacu ccauacgaug caucggagua agcaacagag acuucgucga aggaaugagc      120

ggcggaaccu gggucgacgu aguccuggaa cauggcggcu gcgucaccgu cauggcccaa      180

gacaaaccca ccguagacau agaacucguc acaacaacag uauccaacau ggcugaaguc      240

cgaagcuacu gcuacgaagc cucaauaagc gacauggccu cagacagcag augccccacc      300

caaggagaag cauaccuaga caaacaaagc gauacacaau acguauguaa acgcacacug      360

gucgaccgcg gauggggaaa cggcugcggc cucuucggca aaggcagucu cguaaccugc      420

gccaaauuca caugcagcaa gaaaaugacc ggaaaaucca uacaaccuga aaaccucgaa      480

uauagaauca ugcuaucggu ccacggaagc caacauucgg gcaugaucgg cuacgaaacc      540

gacgaagacc gcgccaaagu agaagucaca ccgaacaguc cccgagcaga agccacccua      600

ggcggcuucg gaucccucgg ccuagacugc gaacccagaa ccggauuaga cuucagcgac      660

uuauacuacc ucaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac      720

auaccccuac cauggcacgc cggcgcagac accggcaccc cgcacuggaa caacaaagaa      780

gcccuagucg aguucaaaga cgcacacgcc aaacgccaaa ccguagucgu acucggaagc      840

caagaaggcg caguacacac cgcccuggcc ggagcccucg aagccgaaau ggacggcgcc      900

aaagggagac ucuucagcgg acaccuaaaa ugcagacuca aaauggacaa acuccgccua      960

aaaggcgugu ccuacucccu augcacagca gccuucacgu ucacaaaagu gccagccgaa     1020

acacuacacg gcacaguaac cgucgaaguc caauacgcgg gaacagacgg cccaugcaaa     1080

aucccagucc aaauggcagu cgacaugcaa acacucaccc ccgucggacg acuaaucacc     1140

gccaacccag ucaucaccga auccacagaa aacucaaaaa ugaugcucga acucgaccca     1200

cccuucggag acuccuacau cgucauagga gucggcgaca aaaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau aggcaaagcc uucgaagcaa caguacgcgg agcaaaacgc     1320

auggccgucc uaggcgacac cgccugggac uucggguccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  4960
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 1 opt

<400>  4960
gggagaaagc uuaccaugag ccaaaaaguc auauaccucg uaaugauccu acucauagcc       60

ccggccuaca gcauccgcug caucggagua ucaaacagag acuucguaga aggcaugagc      120

gggggaacau ggguagacgu aguccucgaa cacggaggau gcguaacagu aauggcacaa      180

gacaaaccga ccguagacau agaauuaguc acaaccacag ucucaaacau ggccgaaguc      240

cgcagcuacu gcuacgaagc gagcauaagc gacauggcaa gcgauucaag augccccacc      300

cagggggaag ccuaccuaga caaacaaagc gacacccagu acguaugcaa acgaacccuc      360

guggaccgcg gauggggaaa cgguugcggc cuauucggga aagggagccu agucacaugc      420

gcaaaauucg caugcagcaa gaaaaugacc gggaaaagca uacaaccaga aaaccuagaa      480

uaccgcauca ugcugagcgu acacgggagc caacacucag gaaugaucgu caacgacacc      540

ggacacgaaa cagacgaaaa ccgagccaaa guagaaauaa ccccuaacuc accacgagcc      600

gaagcgacac ugggcggauu cggcucgcuc ggacuugacu gcgaaccccg gaccggacua      660

gacuucucgg aucuauacua ccucacgaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acauaccccu acccuggcac gcgggcgccg acacaggaac accccacugg      780

aacaacaaag aagcacucgu agaguucaaa gacgcacacg ccaaacgcca aaccguaguc      840

guacuaggaa gccaagaagg cgccguccac acggcacuag ccggagccuu agaagccgaa      900

auggacggcg ccaaaggccg gcuaucaagu gggcaccuga aaugcagacu gaaaauggac      960

aaacucaggc ucaaaggagu gagcuacagc cugugcacgg cagcauucac auucacaaaa     1020

auaccagcgg aaacacucca cgggaccguc acggucgaag uccaauacgc aggcacagac     1080

ggacccugca aaguacccgc gcaaauggcc gucgacaugc aaacgcucac accaguaggc     1140

aggcucauaa cagcaaaccc aguaauuacc gaaagcacag aaaacagcaa aaugaugcua     1200

gaacucgacc caccauucgg agacucauac auagucaucg gcguaggaga gaagaaaaua     1260

acacaccacu ggcaccgauc aggcuccaca aucggcaaag ccuucgaggc aaccgucaga     1320

ggagccaaac gcauggcagu gcucggagac acggccuggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  4961
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       1 opt

<400>  4961
gggagaaagc uuaccaugac aagcguaggc auagucggcu uacugcucac gaccgccaug       60

gcagcggaag uaaccagacg aggaagcgca uacuauaugu aucuggaccg aaacgacgcc      120

ggcgaagcaa ucagcuuccc cacaacgcuc ggcaugaaca aaugcuacau acagaucaug      180

gaccucgggc acaugugcga cgccacgaug aguuacgagu gccccaugcu cgacgaggga      240

gucgaaccag acgacgucga cugcuggugc aacaccacca gcaccugggu gguauacggc      300

acaugccacc acaagaaagg agaagcacgc cgcucucgac gagccguaac acuccccucc      360

cacagcacca gaaaacucca gacaagauca caaacauggc uagaaucacg cgaauacaca      420

aaacaccuca uaagggucga aaacuggaua uucagaaauc ccggauucgc ccucgccgcc      480

gccgcgaucg caugguuacu cggcuccucc accagccaaa aagucauaua ccuagugaug      540

auacuccuca ucgcuccagc auacuccauc cggugcauag gaguauccaa ccgagauuuu      600

guggagggaa ugucgggagg caccuggguc gacaucgucc ucgagcacgg aggcugcgua      660

acagucaugg cacaagacaa acccacaguc gacauagagc uaguuaccac aaccguaucc      720

aacauggccg aaguaagauc guacuguuac gaagcaagca ucagcgacau ggccagcgac      780

agccgcugcc cuacccaagg cgaagccuac cucgacaaac aguccgacac acaauacgua      840

uguaaacgca cccuagucga cagagguugg gguaacggcu gcggacucag cggcaaaggc      900

agccucguga ccugcgcgaa auucgccugc ucaaagaaaa ugaccggcaa auccauacaa      960

cccgaaaacc uagaauaccg caucaugcuc ucgguccacg gaucacagca cucaggcaug     1020

auagucaaug acacaggaca cgaaaccgac gaaaaccgag cuaaagucga aaucacccca     1080

aacagcccac gcgccgaagc cacgcucgga ggcuucggau cacuagggcu agacugcgaa     1140

ccccgcacag gacuagacuu cagcgaccuc uacuaccuga caaugaacaa caaacacugg     1200

cucguucaca aagaaugguu ccacgauauc ccccuacccu ggcacgcagg agccgacaca     1260

ggaaccccac acuggaacaa caaggaagcc cucguagagu ucaaagacgc acacgccaag     1320

cgacaaaccg ucguaguauu aggcucgcaa gaaggcgcag uacacacagc ccucgccggc     1380

gcacucgaag cagaaaugga cggcgccaaa gggcgacuaa guagcggcca ucucaaaugc     1440

cgacucaaaa uggacaaacu ccgacuaaaa ggcgucucau acucccuaug caccgcagca     1500

uucaccuuca ccaaaauccc ggccgaaaca cuccacggaa ccgucaccgu cgaaguacaa     1560

uacgcaggaa cggauggccc guguaaaguu cccgcgcaaa uggccgucga caugcaaacg     1620

cuuacaccag ucggcagacu cauaacagcc aacccaguaa uaaccgaaag cacagaaaau     1680

agcaagauga ugcucgaacu agacccaccc uucggagacu ccuacauagu aaucggcgua     1740

ggagaaaaga aaaucaccca ccacuggcac cgaagcggaa gcacgaucgg caaagcauuu     1800

gaagccaccg ugcgcggcgc caagagaaug gccguacucg gcgacacagc cugggacuuc     1860

gguuccgucg gaggcgcacu aaacucacug ggcaaaggca uacaccaaau cuuuggagca     1920

gccuucaaaa gccucuucgg aggcauguca ugguucuccc agauacucau cggcacccua     1980

cucauguggc uaggccugaa caccaagaac gggucaauau cacuaaugug ccuagcccuc     2040

ggcggaguac uaauauuccu gagcaccgcc guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4962
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 1
       opt

<400>  4962
gggagaaagc uuaccaugac gagcgucggc aucgucggac ugcuucucac aaccgcgaug       60

gcagccgaag uaacccgcag aggauccgca uacuacaugu accuagaccg aaacgacgcc      120

ggagaagcca ucagcuuccc aaccacccug ggaaugaaua aaugcuacau acagaucaug      180

gaccugggcc auaccugcga cgcgaccaug uccuaugagu gcccaaugcu agacgaagga      240

guagagcccg augacgucga cugcuggugc aacacaacuu cgaccugggu cguguacgga      300

accugccacc acaagaaagg cgaagcacgg cgaucaagac gagccgucac acuaccaagc      360

cacagcaccc gcaagcuaca aacacgaagc cagacauggc uagaaucccg agaguacacc      420

aaacaccuaa uccgaguaga gaacuggauc uuccgcaacc caggcuucgc acuugccgcg      480

gccgcaaucg ccuggcugcu cggcagcagc acaagucaaa aggucaucua ccuggucaug      540

auacuccuaa uagcgccggc cuacagcauc cguugcaucg gcgucuccaa ccgagacuuc      600

guugaaggca ugucaggagg cacaugggua gacguaguac ucgagcacgg aggcugcgua      660

accgugaugg cgcaggacaa accaacagua gacaucgagc uagucaccac aaccgugucg      720

aacauggcug aagucaggag cuacugcuau gaagcaucaa uaucagacau ggcaagcgac      780

uccagaugcc ccacacaagg cgaagccuac cucgacaaac aaucugacac ccaauacgug      840

ugcaaaagga cccuaguaga ccggggcugg ggcaacgggu gcggccuaag cggcaaaggc      900

agccuaguaa ccugcgccaa auucgcaugc uccaagaaaa ugacaggcaa auccauccaa      960

ccagaaaacc uagaauacag aaucauguua ucaguccacg gaagccaaca cucaggcaug     1020

aucgucaacg acaccggaca cgaaacagac gaaaaccgcg cgaaaguaga aauaacccca     1080

aacucaccca gagcagaagc cacacugggc ggcuucggcu cccucggacu ggacugcgaa     1140

ccccgaaccg ggcuagacuu cucagaccua uacuaccuca caaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacauc ccacuccccu ggcacgcagg cgccgacacc     1260

ggcacaccac acuggaauaa caaagaagcc uuaguggagu ucaaagacgc acaugccaaa     1320

agacaaaccg ucgucguacu aggcagucaa gaaggcgcag uacacacagc gcucgcgggc     1380

gcgcuggaag ccgaaaugga cggagcaaaa ggcagguuau caagcggcca ccucaaaugc     1440

cgacuaaaaa uggauaaacu acgccucaaa ggcguaagcu acagccucug cacagcagca     1500

uucaccuuca ccaaaauacc cgccgaaaca cugcacggca caguaacagu cgaaguccaa     1560

uacgcaggca ccgacggacc augcaaaguc cccgcccaga uggccgucga caugcaaacc     1620

cucacucccg uagggcgcuu aaucaccgcc aacccaguca ucacagaauc cacagaaaac     1680

aguaaaauga ugcucgaacu cgacccgccu uucggagacu ccuacaucgu aaucggcgua     1740

ggcgaaaaga agaucaccca ucacuggcac agaucaggua gcaccaucgg caaagcguuc     1800

gaagcaaccg ugcgcggcgc gaaacgcaug gcaguucucg gcgacacugc augggacuuu     1860

ggcucaguag gaggcgcacu caacagccua ggaaaaggaa uacaccaaau auucggcgcc     1920

gcauucaaaa gccuauucgg cggaauguca ugguucagcc aaauacucau uggcacccuc     1980

cucauguggc ugggccucaa cgccaaaaac ggcuccauau cgcucaugug ccucgcacua     2040

ggcggcguau uaauauuccu cucaacagcu gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4963
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 1 
       opt

<400>  4963
gggagaaagc uuaccaugac cuccauaggc auaaucggcc uacugcuuac gacagcaaug       60

gccgcagaaa uaacacgacg gggcuccgcc uacuacaugu accucgaccg cucggacgcc      120

ggaaaagcaa ucaguuucgc uacaacccuc ggcguaaaca aaugccacgu ccaaaucaug      180

gacuuaggcc acaugugcga cgcgacuaug uccuacgaau gccccauguu agacgagggc      240

gucgaacccg acgacgucga cugcuggugc aacaccaccu cgacaugggu agucuacggc      300

acuugccauc acaagaaagg agaagcacgc cguucaagac gagcagucac ccuacccagc      360

cacagcacac gaaaacuaca aacccgcucc caaaccuggc uagaaagccg cgaauacacc      420

aaacaccuca uaaaagucga aaacuggaua uuccgaaacc ccggcuucgc acucgucgcc      480

guagccaucg cauggcuccu aggauccagc accagccaga aagucauaua ccuagucaug      540

auucuacuca ucgcaccggc auacuccaua cgcugcaucg gaguaagcaa cagagacuuc      600

gucgaaggua uguccggcgg cacuuggguc gacgucgucc ucgaacacgg aggaugcgug      660

acagucaugg cacaagacaa accaacagug gacaucgaac ucguaaccac caccgucagc      720

aacauggccg aaguccgaag cuacugcuac gaagccagca ucagcgacau ggcuucagac      780

agccgcugcc caacgcaagg ggaagccuac cucgacaaac aaucagacac ccaauacgua      840

ugcaaacgca cccuggucga ucgcggaugg ggcaacgggu gcggccuuuc uggcaagggg      900

ucccuaguaa ccugcgcaaa auucaccugu ucaaagaaaa ugaccggcaa auccauccaa      960

ccugaaaacc ucgaauaccg uauaaugcuc aguguccacg gaucccaaca cagcggaaug     1020

aucggcuacg aaacagacga agaccgcgcc aaaguagaag uaacccccaa cucgccacga     1080

gccgaggcca cacuaggcgg uuucggcucc cuaggacucg acugcgaacc ucgcacaggg     1140

cucgacuucu ccgaccuaua cuaccugaca augaacaaca aacacugguu aguccauaag     1200

gaaugguucc augacauacc ccuccccugg cacgcuggcg ccgacacagg cacaccgcac     1260

uggaacaaca aagaagcucu aguagaauuu aaagacgccc acgccaaacg acaaacaguc     1320

guuguccuag gcucccaaga gggagcagua cacacagccc uagcgggcgc ccuagaagcc     1380

gaaauggacg gcgccaaagg ccgccucuuc uccggccacc ucaaaugucg ccucaagaug     1440

gacaaacucc gccuaaaagg cguaucuuau agccuaugca cagcagcauu cacauucaca     1500

aaaguaccag ccgaaacgcu ccacggaacg gucacagucg aaguccaaua cgccggcacc     1560

gacggaccgu gcaagauccc aguacaaaug gcaguagaca ugcagacccu aacaccaguc     1620

ggccgccuga uaacugccaa cccagucaua accgaaucga ccgaaaacuc aaagaugaug     1680

cuugagcuug acccacccuu cggcgacucc uacauuguaa ucggcgucgg ggacaagaaa     1740

aucacccacc acuggcacag aagcggaucc accauaggaa aagccuucga agcaaccgua     1800

agaggagcca aaagaauggc aguccucggc gauacagcuu gggacuucgg aagcgucgga     1860

ggcgucuuca acucccucgg uaaaggcauc caccaaauau ucggcgcagc auucaagucc     1920

cuguucggcg gaaugucaug guucucacag auacuaaucg gaacacugcu aguauggcua     1980

ggccucaaca ccaaaaacgg cagcaucagc cuuacgugcc uagcacuggg cggagucaug     2040

aucuuucucu ccaccgccgu auccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4964
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 1 
       opt

<400>  4964
gggagaaagc uuaccaugac aucaguaggg auaguaggac uccuccugac aacagccaug       60

gccgccgaag ucacaagacg uggcagcgcg uacuacaugu accuggauag aaacgacgca      120

ggcgaagcaa ucaguuuccc cacaacacug ggcaugaaca agugcuacau ccaaaucaug      180

gaccugggcc acaugugcga ugccaccaug agcuacgaau gccccaugcu agacgaaggc      240

gucgaacccg acgacgucga cugcuggugc aacacaacaa guacgugggu cguguacggc      300

acaugucacc acaaaaaggg agaagcucgg cggucccgcc gagccgucac gcuccccagc      360

cacagcacaa gaaaacucca aacacgaagc caaacauggc uagaaucaag agaauacacc      420

aaacaccuca uaagagugga aaacuggauc uucagaaacc caggcuucgc acuagccgca      480

gccgcaaucg cgugguuacu uggcuccagc acuucucaga aagucauaua ccugguaaug      540

auacuacuaa uagccccggc auacucgaua cgaugcaucg ggguaaguaa cagggacuuc      600

gucgaaggaa uguccggagg cacaugggua gacguagucc uagaacacgg cggaugcguu      660

acgguaaugg cccaagacaa accaaccgua gacaucgaac uggucacaac aaccguaagc      720

aacauggcag aggucagaag cuacugcuac gaagccucaa uaagcgacau ggcaagcgau      780

ucccggugcc caacucaagg ggaagcauac cuagacaaac aauccgacac acaauacgua      840

ugcaaacgca cacuuguaga cagaggcugg gggaacggcu gcggacuauc cggaaaaggc      900

ucguuaguga caugugcaaa auucgcaugc agcaagaaaa ugacagggaa aucaauacaa      960

cccgaaaacc uagaauaucg gaucaugcuc uccguacacg gcucacaaca cagcggaaug     1020

aucguaaacg acaccggaca cgaaacagau gaaaaccgag cuaaaguaga aaucaccccc     1080

aacagcccaa gagcagaagc aacacucgga ggauucgguu cacuuggacu cgacugcgag     1140

cccagaaccg gccucgacuu cucagaccua uacuaccuaa cgaugaauaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccccugccgu ggcacgcagg ggcggacaca     1260

ggaaccccac acuggaacaa caaagaagcg cuggucgagu ucaaagacgc ccacgcuaaa     1320

cgccaaaccg uaguaguacu cggaagccag gaaggagccg uacacaccgc ccuggccggg     1380

gcacucgaag cagaaaugga cggcgccaaa ggccgccuaa gcagcggaca ccuaaaaugc     1440

cgucugaaaa uggacaaauu gcgacucaag ggaguauccu acucccuuug caccgcagca     1500

uucacauuca caaaaauacc cgccgaaaca cuacauggca caguaacagu agagguccaa     1560

uacgcaggca cggacggucc augcaaagua cccgcgcaaa uggccgucga caugcaaaca     1620

cugacuccag ucggcagacu cauaaccgca aaccccguaa uaaccgaaag uaccgaaaac     1680

agcaaaauga uguuagagcu agacccaccc uucggcgaca gcuacauagu aauaggaguc     1740

ggcgagaaaa agauaaccca ccacuggcac cgcagcggaa gcaccaucgg caaagccuuc     1800

gaagccacag uccgcggggc caaacgaaug gcaguccugg gcgacacagc augggacuuc     1860

gggucagucg gcggcgcacu aaacagccuc gggaaaggca uacaccaaau cuucggugca     1920

gcuuucaaaa gccuauucgg ugggauguca ugguucagcc aaauacucau aggcacccuc     1980

cucauguggc ugggccucaa caccaaaaac ggcucaauaa gccuaaugug ccuagcacua     2040

ggcggcgucc ugauauuccu guccacagcc guauccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4965
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       1 opt

<400>  4965
gggagaaagc uuaccaugac cagcgucgga auagucggac uccuccucac aacagcaaug       60

gccgcggaag ucacccgacg cggcagcgcu uacuacaugu accucgaccg aaacgacgcc      120

ggcgaagcca ucagcuuccc caccacccug ggcaugaaca aaugcuacau acaaauaaug      180

gaccuuggcc acaugugcga cgcgaccaug agcuacgaau gccccaugcu cgacgaagga      240

guggagccag acgauguaga cuguuggugc aacacaacau caaccugggu cgucuacggc      300

acaugccacc acaagaaggg agaagccaga cgcagccgca gagcaguaac ccuaccgucc      360

cacucuacca gaaaacucca aacacgaagc cagacauggu uagaaagccg cgaauacaca      420

aaacaccuca ucagagucga gaacuggaua uucagaaacc caggcuucgc auuagccgca      480

gccgcaaucg ccuggcuccu agggagcuca accucacaaa aaguaauaua ccuagucaug      540

auacuacuca uagccccggc cuacagcaua cgaugcauag gugucucaaa cagggacuuc      600

gucgaaggca ugagcggagg cacgugggug gacauaguac ucgaacacgg cggcugcgug      660

accgucaugg cacaagacaa accgacagua gacauagaau uggugacaac cacagucucc      720

aacauggccg aagugcguuc auacugcuac gaagcaagca ucagcgacau ggccucagac      780

agccgaugcc caacccaagg cgaagccuac cuagacaagc aaagcgacac acaauacguc      840

ugcaagcgga cgcuagugga ccgaggcugg ggcaacggcu gcggacuauu cggcaaagga      900

agccucguaa caugcgcgaa auucgcgugc agcaagaaaa ugaccggcaa auccauccaa      960

cccgaaaacc ucgaauacag aauuaugcuc uccguacacg gcagccaaca cuccggcaug     1020

auuguacaag auacaggcca cgagacagac gaaaacagag cgaaagucga aauaaccccc     1080

aacucgcccc gggccgaagc aacucuagga ggauucggau cgcuagggcu ugacugcgaa     1140

ccccgaacag gacuagacuu cuccgaccug uacuaccuaa caaugaacaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacauc cccuuacccu ggcacgcagg cgccgacaca     1260

ggaaccccac acuggaacaa caaagaagcc uuaguggagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg uaguaguacu aggaagccaa gaaggagcag uacacacagc cuuggccgga     1380

gcguuagaag ccgaaaugga cggggcaaaa ggcaggcuua gcucgggaca cuuaaaaugc     1440

agacuaaaaa uggacaagcu ccgccugaaa ggaguauccu acucccuaug cacagccgcg     1500

uucacauuca cgaaaauacc cgccgaaacu uuacacggca cggucacagu cgagguccaa     1560

uacgcaggca ccgacgggcc augcaaagua cccgcccaaa uggccgucga uaugcaaacc     1620

cuaaccccag uaggaaggcu uauuaccgcc aacccaguca uaacagaaag cacagaaaac     1680

ucaaaaauga ugcuagaacu agauccaccc uucggagacu ccuacaucgu cauaggcguu     1740

ggagaaaaga aaaucacaca ccacuggcac agaucaggaa gcaccauagg caaagccuuu     1800

gaagcgacag uacgcggcgc aaagagaaug gccguacuug gcgacacagc cugggacuuc     1860

ggcucagucg ggggcgcgcu gaacagccua ggcaaaggga uacaccaaau auucggagca     1920

gcauucaaau cccucuucgg cggaauguca ugguucucac aaauccucau cggaacccua     1980

cugauguggc uaggccuaaa caccaaaaac ggcagcaucu cacucaugug ccuggcccua     2040

ggcggagucc ucaucuuccu cuccaccgcc guaucggccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4966
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 1
       opt

<400>  4966
gggagaaagc uuaccaugac cagcguagga aucgucgggc uacuacucac aacagcaaug       60

gcggccgagg ucacccgcag aggauccgcc uacuacaugu accuagaccg aaacgacgca      120

ggcgaagcga ucagcuuccc aacuacacua ggaaugaaca aaugcuacau acaaauaaug      180

gaccuuggac acacaugcga cgccaccaug agcuacgaau gcccaaugcu ggacgaggga      240

guugagcccg acgaugugga cugcuggugc aacaccacga gcacgugggu ugucuauggc      300

acaugccacc acaagaaagg cgaagccaga cgcagccgac gcgcaguaac acucccauca      360

cacucaacaa gaaaacuuca aacaagaagc caaaccuggc uggaaagccg agaauacaca      420

aaacaccuga uacgagucga aaacuggauc uuccggaacc ccggauucgc acucgcggcu      480

gcagccaucg ccuggcuacu cggcuccagc acaucccaaa aagucaucua ccucgugaug      540

auauuauuga uugcacccgc auacucaauc agaugcaucg gcguaagcaa ucgugauuuc      600

guggagggga ugucaggcgg caccugggug gacgucguac uagaacacgg gggaugcguc      660

acaguuaugg cccaagacaa accaacaguc gacaucgaac ugguaaccac aaccguaagc      720

aauauggccg aggugcgauc auacuguuac gaggccagca uaagcgacau ggcuucagac      780

ucccgcugcc ccacccaagg agaagcguac cuagacaaac aaucagacac acaauacgua      840

ugcaaaagga cccuagucga ccgaggaugg ggcaacggau gcgggcuauu cggcaagggc      900

agccuaguaa cuugcgccaa auucgcaugc uccaagaaaa ugacaggcaa aagcauacaa      960

ccagaaaacc uagaauacag aaucaugcua ucgguacacg gaagccaaca cagcgggaug     1020

auaguacaag acacgggaca cgaaacagac gaaaacaggg ccaaaguaga gauaacgccc     1080

aacagucccc gggcagaagc aacacuaggc ggcuucggau cacuaggccu agacugcgaa     1140

ccacgcacag gccuagacuu cagcgaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cugguacaua aagaaugguu ccacgacaua ccguuaccgu ggcacgcagg cgcagacacc     1260

ggcacacccc acuggaacaa caaagaggcc cucguagagu uuaaagacgc ccacgcaaaa     1320

cggcaaacag ucguaguccu aggaucgcaa gagggcgcag uccacacagc acuagcaggc     1380

gcccuagaag ccgaaaugga cggagccaaa ggacgacucu ccuccggcca ccuaaaaugc     1440

agacucaaga uggauaaacu ccgacuaaaa ggcgucuccu acucgcucug cacagcggca     1500

uuuacauuca ccaaaauacc agccgaaacc uuacacggaa ccguuaccgu cgaaguacag     1560

uacgcaggaa cagacgggcc augcaaaguc ccggcacaga uggccgucga caugcaaacc     1620

cucaccccag ucggccgacu caucacggcc aaccccguga ucacagaauc gaccgaaaau     1680

ucaaaaauga ugcuagaacu ugacccaccc uucggagaca gcuacaucgu cauaggcguc     1740

ggcgagaaga aaaucaccca ucacuggcac cgcagcggaa gcaccauagg aaaggccuuc     1800

gaggcaaccg ugagaggagc gaaacgaaug gccguacucg gcgacacugc augggacuuc     1860

ggcuccgucg gcggagcucu aaacucccug ggaaaaggaa uccaccaaau auucggagcc     1920

gccuucaaaa gccuauucgg aggaaugucc ugguucucac aaauacuaau aggcacccuc     1980

cugauguggc uaggacuaaa cgcaaagaac ggguccauaa gccugaugug ccuggcgcuc     2040

gggggcgucc uaaucuuccu auccacagca guauccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4967
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 1 
       opt

<400>  4967
gggagaaagc uuaccaugac aagcguagga aucgucgggc uccugcuaac gacagcaaug       60

gcagcagaag ucacucgacg aggcuccgca uacuacaugu accuggaccg aaacgacgcc      120

ggagaagcca uauccuuccc aaccacacuu ggcaugaaca aaugcuauau ccaaauaaug      180

gaccucggac acaugugcga ugccacaaug ucauacgaau gcccaaugcu cgacgaaggc      240

guggaaccag augacgucga cugcuggugc aacaccacaa gcaccugggu cguauacggu      300

acaugccacc acaagaaagg cgaagcacgg aggagccgac gagcaguaac ccuaccaagc      360

cacuccacuc gcaaacucca aacccgaagc caaaccuggc uagaaagccg agaauacacc      420

aaacaccuaa uacggguaga aaacuggauc uuccgaaauc ccggcuucgc acuagccgcc      480

gcggcaaucg cauggcucuu aggcucaagu acaagccaaa aaguaaucua ccucgucaug      540

auucuacuaa ucgcccccgc cuacuccaua cgcugcaucg gaguaagcaa cagagacuuc      600

gucgaaggga ugucaggcgg uaccugggug gacgucguac uagaacacgg cggcugcgua      660

accgucaugg cccaagacaa accaacggua gacaucgaac ucgucaccac cacagucagc      720

aacauggccg aagugcgcuc cuauugcuau gaagcaucaa uaucagacau ggcuucagac      780

ucacgaugcc caacacaagg agaagccuac cuagacaaac agucagacac ccaauacgua      840

ugcaaacgaa ccuuagucga ucgcggaugg ggaaacggcu gcggccucuu cgggaaaggc      900

ucgcuaguga caugcgccaa auucgcaugc uccaagaaaa ugaccggcaa aucaauccaa      960

ccagagaacc uagaguauag gauaaugcuc ucaguccacg gaucgcaaca uagcggaaug     1020

auaguccaag acacgggaca cgagacggac gagaaccgcg caaaagucga aauaacacca     1080

aacagccccc gagcggaagc gacacuaggc ggguucggaa gccuaggacu agacugcgag     1140

ccacgaacgg gccuggacuu cucagaccua uacuaccuca ccaugaacaa caaacauugg     1200

cuaguccaca aagaaugguu ccacgacauc ccccucccau ggcacgcggg ugcagacaca     1260

ggcacucccc acuggaacaa caaagaggcc cuggucgaau uuaaagacgc ucacgcgaaa     1320

aggcaaaccg ucguaguacu gggcucacaa gaaggcgcgg uccauacagc ccucgcaggc     1380

gcauuagaag ccgaaaugga cggagcaaaa ggacgccuau ccucaggcca ccucaaaugc     1440

cgacucaaaa uggacaaacu acggcugaaa ggaguauccu acagccucug cacagccgca     1500

uucaccuuca ccaaaauccc cgccgaaacc cuacacggga ccguaacagu agaaguacaa     1560

uacgcaggga cagauggccc augcaaaguc ccggcccaga uggccgucga caugcaaaca     1620

cuaacaccgg uuggacgccu caucacagcc aaccccguca uuacagaauc aacagagaac     1680

uccaaaauga ugcucgaacu agacccaccc uucggagauu cauacaucgu cauaggcguc     1740

ggagaaaaga aaauaacuca ccacuggcac cgcagcggca gcaccaucgg caaagcauuc     1800

gaagccacag uccgaggagc aaaaaggaug gcaguacucg gagacaccgc augggacuuu     1860

gguagcgucg gcggcgcccu aaacucacua ggaaaaggaa uacaucaaau cuucggagca     1920

gcauuuaaaa gccucuucgg uggaaugagc ugguucuccc aaauacuaau cggaacccuc     1980

cugauguggc uaggacugaa caccaaaaac ggaagcauca gccugaugug ccucgcgcuc     2040

gggggcguac uaaucuucuu aagcacagca guaucggccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4968
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4968
gggagaaagc uuaccaugac aagcguugga aucgucgggc ugcuacucac gaccgccaug       60

gccgcugaag ucacgcgcag gggcucagca uacuacaugu accucgacag aaacgacgcg      120

ggcgaagcaa ucagcuuccc aacaacacuc ggcaugaaca aaugcuacau acaaauaaug      180

gaccucggac acaugugcga cgcaacaaug ucguacgaau gccccaugcu cgacgaagga      240

guugaacccg acgacguaga cugcuggugc aacacaacaa guaccugggu cguguacgga      300

accugccacc acaagaaagg agaagcuagg cggucacgac gcgcaguaac ccugccaagc      360

cacuccacga gaaaacuaca aacccgcagc caaacauggc uggaaucacg cgaauacacc      420

aaacaccuca uccgcgugga aaacuggauu uucagaaacc caggcuucgc gcuagccgcc      480

gccgccauag cauggcuacu cgggucaucc acaagccaaa aagucauaua ccucgucaug      540

auccuguuaa ucgccccagc guacagcaua cggugcaucg gcgucagcaa ucgagacuuc      600

guggaaggca uguccggcgg aaccuggguc gacaucgucc ucgagcacgg agggugcgua      660

accgucaugg cacaagacaa accaacagua gacauagaac ucgugacaac aacaguaucu      720

aauauggccg aaguccgcuc cuacugcuac gaagccagca ucucagacau ggccuccgac      780

ucacgcugcc caacgcaagg ggaagcauac cucgacaaac aauccgacac ccaauacgua      840

ugcaaaagaa cacucguaga ccgagggugg ggaaacggau gcggccuauu cggaaaaggc      900

agccucguaa ccugcgccaa auucgcgugc uccaagaaaa ugacgggcaa aagcauccaa      960

cccgaaaacc ucgaauacag aaucaugcug uccguccacg gcucccaaca cuccgggaug     1020

aucguaaacg acacgggaca cgaaaccgac gaaaaccgag ccaaaguaga aaucacccca     1080

aacaguccca gagccgaagc caccuugggu gguuucggaa gccucggccu cgacugcgaa     1140

ccgagaacag gccuggacuu cagcgaccuc uacuaccuca caaugaacaa caaacacugg     1200

uuaguccaua aagaaugguu ccacgacaua ccucuccccu ggcacgccgg cgcagacacc     1260

ggaaccccac acuggaacaa caaagaggca cuuguagagu ucaaagacgc ccacgccaaa     1320

cgacaaaccg ucguaguccu cggaucacaa gaaggcgcgg uacacaccgc acucgcgggg     1380

gcccuggaag cagaaaugga cggcgccaag ggcagacuca gcuccgggca ccucaaaugc     1440

agacugaaaa uggacaagcu ccgacucaag ggagucucuu acucacuaug caccgccgcg     1500

uucacauuca cuaaaauccc agccgaaaca cuccacggua ccgucaccgu agaaguccag     1560

uaugcaggca cugacggccc gugcaaagug cccgcccaaa uggccguaga caugcaaaca     1620

cucaccccag uaggaaggcu aaucacugca aaccccguga uaacagaauc aaccgaaaac     1680

ucaaaaauga ugcuagaacu agacccaccc uucggagacu ccuacaucgu cauaggagug     1740

ggggaaaaga aaaucacaca ccacuggcac agguccggcu ccacacucgg caaagccuuc     1800

uccaccaccc uaaaaggcgc acaacgccua gcagcccuag gagacacggc gugggacuuc     1860

ggguccauag gcggcguauu caacuccauc ggacgcgcag uccaccaagu cuucggcgga     1920

gccuuccgaa cccuauucgg cggaaugagc uggauaaccc agggacugau gggagcacua     1980

cugcuaugga ugggcgucaa cgcacgcgac agaagcauag cacuagccuu ccucgccacu     2040

ggcggaguac ucguauuccu ggcaacaaac guccacgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4969
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4969
gggagaaagc uuaccaugac cagcgucgga aucgucggac uccugcuaac aacagcaaug       60

gcagccgaag ucacgcgaag gggaucggca uacuacaugu aucucgaccg caacgacgcg      120

ggcgaagcca ucagcuuccc aacaacgcug ggcaugaaca aaugcuacau acaaauuaug      180

gaccuaggac acacaugcga cgcaacaaug agcuacgaau gccccaugcu cgacgagggc      240

guugaacccg acgacgucga cugcuggugu aauacgacau ccacaugggu aguauacggg      300

accugccacc acaagaaagg ggaagcgcgc cgguccagga gggccgucac ucucccgagc      360

cacaguacca ggaaacuaca aacccgcagc caaaccuggc ucgaaagccg ugaauauaca      420

aaacaccuaa uucgcguaga gaacuggauc uuccgcaacc ccggauucgc ccuggcagca      480

gcagcaauag ccuggcuguu gggcucaagc acaagccaaa aggucauaua ccugguaaug      540

auccuacuaa uagcaccagc auacuccauc agaugcauag gcgugagcaa ccgagacuuc      600

guugaaggaa ugucgggcgg gacauggguc gacguagucc uagaacacgg aggcugcgua      660

acggucaugg cucaagacaa gcccacgguc gacauagagc uaguuacuac aacaguaagc      720

aacauggccg aagucaggag cuauugcuac gaagccucca ucucagacau ggcauccgac      780

ucccggugcc ccacccaagg agaagcauac cucgacaaac aaagcgacac gcaauacguc      840

ugcaaaagga cccucgucga ccgcggaugg ggcaacggau gcgggcuauu cggaaaagga      900

ucccucguga ccugcgccaa auucgccugc uccaagaaaa ugacaggcaa aagcauacaa      960

cccgagaauc uggaauacag aauuaugcuc agcguacacg gcucacagca cagcggcaug     1020

aucguaaacg acaccggaca cgaaacggac gaaaaccgag caaaagucga aauaacacca     1080

aacagcccuc gcgcggaagc gacccuggga ggauucggga gccuaggacu ggacugcgaa     1140

ccccgcacgg gccuagacuu cagcgaucuc uauuaccuaa ccaugaacaa caaacacugg     1200

cuagugcaca aagagugguu ccacgacauc ccacucccgu ggcacgccgg agccgacaca     1260

ggaaccccgc acuggaacaa caaagaggcc cuuguagagu ucaaagacgc acaugcgaaa     1320

agacaaacag ucguaguccu gggcucacaa gaaggagccg uacacacagc acuagcaggc     1380

gcucucgaag ccgaaaugga cggagccaaa gggcgccuaa gcuccggcca ccugaaaugc     1440

cgccuaaaaa uggacaaacu acgccucaag ggaguaucau auucccuaug caccgcagcc     1500

uucacauuca caaaaauccc agccgaaaca cuacacggca caguaacagu cgaaguacaa     1560

uacgcaggaa cagacggccc augcaaaguc cccgcccaaa uggcgguuga caugcaaacc     1620

cuaaccccag ucggacgccu gaucacagca aacccaguca ucaccgaauc cacagagaac     1680

ucaaaaauga ugcucgaacu agacccgccc uucggcgaua gcuacaucgu aauaggggua     1740

ggagaaaaga aaaucacaca ucacuggcac cgcagcggaa gcacccuagg caaagcauuc     1800

ucgaccacac ugaaaggagc acaacggcuc gcagcccucg gagacaccgc cugggacuuc     1860

ggauccauag ggggagucuu caacucgauc ggccgcgcag uacaucaagu auucggcggc     1920

gcguucagga cacuauucgg aggaaugagc uggaucacac aaggccuaau gggagcccua     1980

cuacucugga ugggcgucaa cgcacgagac cggagcauag cccuagcauu ccucgccacc     2040

ggaggcguac ucguauuccu agcaacgaac guacacgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4970
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 1 opt

<400>  4970
gggagaaagc uuaccaugac cagcaucgga auaaucggac uccuccuaac gaccgcaaug       60

gcagcagaaa uaacacgacg aggcucagcc uacuacaugu accuggaccg cagugacgca      120

ggaaaagcga ucucguucgc aacuacccuc ggcgucaaca aaugccacgu ccaaaucaug      180

gaccucgguc acaugugcga cgcaaccaug uccuacgaau gcccaauguu agaugaaggu      240

guagaaccag acgacguaga cugcuggugc aacacgacgu caaccugggu cgucuacggu      300

accugccauc acaagaaagg cgaagcccgc cgcagcagac gcgccguaac ccuaccaagc      360

cacucaacac gaaaacucca gaccagauca cagacguggc uggaaucccg agaauauacc      420

aaacaccuaa uaaaagucga aaacuggaua uucagaaauc ccggauucgc ccucgucgca      480

guagcaaucg cuuggcuacu cggauccagc acaagccaaa aaguaaucua ucucguaaug      540

auacuccuaa uagcacccgc auacuccaua agaugcaucg gcguaagcaa ccgagacuuc      600

gucgaaggaa ugucgggcgg cacaugggua gacgugguac uagagcacgg aggaugugua      660

acggucaugg cacaagauaa accgaccguc gacauagaac ucguaacaac caccguaucc      720

aacauggccg aagucagaag cuacuguuac gaagccucca ucucagacau ggccucagac      780

ucccgcugcc ccacacaggg cgaagcauac cuagacaagc aauccgacac acaauauguc      840

ugcaaacgaa cgcuagucga ccgaggaugg gggaacggau gcggacucuu ugguaagggc      900

ucacucguaa ccugcgccaa auuuaccugc agcaagaaaa ugaccggaaa aagcauccaa      960

ccggaaaacc uagaauacag aauuaugcuc ucgguccacg gcucacaaca cagcgggaug     1020

auaggcuaug aaaccgacga agacagagcc aaaguagagg ucacaccaaa cagccccagg     1080

gcagaagcaa cccucggcgg cuucggaucc cucggccucg acugugaacc ccgaaccggc     1140

cuggacuucu ccgaccucua cuaccugacc augaacaaca agcacugguu aguucacaaa     1200

gaaugguucc augacauccc ccuacccugg cacgccggcg cagacaccgg caccccacac     1260

uggaacaaca aagaagcccu agucgaguuc aaagacgcac acgccaaacg gcaaacaguc     1320

guaguccuug gcucccaaga aggagccgua cacaccgcac ucgccggagc acuagaagca     1380

gaaauggacg gugccaaggg ccgacucuuc agcggacacc uaaaaugccg ccucaagaug     1440

gacaagcuaa gacucaaagg ggucuccuac ucucucugca cugcagccuu caccuucacg     1500

aaaguccccg cagaaacccu ccacgggacu gucaccguag aaguacaaua cgccgguacc     1560

gacggcccau gcaaaauccc aguacaaaug gcaguagaca ugcagacacu uacacccgua     1620

ggacgccuaa uaacagcaaa ccccgucaua accgaaagca cagaaaacuc caaaaugaug     1680

cuagaacuag acccucccuu cggagacagc uacauaguga ucggcgucgg cgacaagaaa     1740

aucacacacc acuggcacag aagcggcagc acccuaggca aagcauucuc uacaacccuc     1800

aaaggcgccc aacgacuagc agcacucgga gacacagccu gggacuucgg aagcaucgga     1860

ggcgucuuca acagcauagg acgcgccgua caccaagucu ucggaggcgc uuuccgcacc     1920

cucuucggcg gaaugagcug gaucacccaa gggcugaugg gcgcacuacu acucuggaug     1980

ggaguaaacg cacgggacag aagcaucgcc cuggcuuuuc ucgccaccgg cggcguccuc     2040

gucuuccuag caacgaacgu acaugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  4971
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 1 opt

<400>  4971
gggagaaagc uuaccaugac cuccgucggc auaguaggcc uacuacucac caccgccaug       60

gccgccgaag ucacgcgcag aggaagcgca uacuacaugu accuggaccg aaacgaugcc      120

ggcgaagcga ucuccuuccc aaccacucuc gggaugaaca aaugcuauau ccaaaucaug      180

gaccuaggac acaugugcga ugcaaccaug uccuacgagu gcccaaugcu agaugaagga      240

gucgaaccag acgacgugga cugcuggugc aacaccaccu cgacaugggu agucuacggc      300

acaugccacc auaagaaggg ggaagccaga cgcuccagac gagcaguaac gcuccccuca      360

cacucaacga gaaaacucca aaccagaagc cagacauggc ucgaaucacg cgaguacacc      420

aaacaccuca uccgcgucga aaacuggauc uuccgaaauc cuggcuucgc ccuagcagca      480

gccgccauag ccuggcuacu cggcagcagc accagccaaa aaguaaucua ucuaguaaug      540

auccuacuaa ucgcacccgc cuacagcaua cgcuguaucg guguaucaaa ccgcgacuuu      600

gucgaaggaa ugucaggcgg cacauggguc gacguagucc uagaacacgg cggcugcguc      660

acaguaaugg cacaagacaa accaacgguc gacauagagc uaguaacaac aacgguaucc      720

aacauggccg aaguccgauc auacugcuac gaagccucca ucucagacau ggcaucggac      780

ucccgcuguc cgacacaagg agaagcguac cuagacaaac aaucggacac ccaauacgua      840

ugcaaacgaa cucuaguaga ccgcggcugg ggaaacggau guggccucuu cgguaagggc      900

ucacucguga ccugcgcaaa auucgcaugc agcaagaaaa ugaccggcaa auccauccaa      960

ccugaaaacc uagaauaccg aauaaugcua ucaguccacg guucacaaca cuccggaaug     1020

aucguaaacg acaccggcca cgaaacagac gaaaaccgag ccaaaguaga aauaaccccc     1080

aacagcccac gagcggaagc aacccuaggg ggauucggcu cacuggggcu ggacugugaa     1140

cccagaacgg gccuagacuu cagcgaccuc uacuaccuaa caaugaacaa uaaacacugg     1200

cuaguacaca aagaaugguu ccacgacauc ccccuccccu ggcacgcagg cgccgauacc     1260

ggcacucccc acuggaacaa caaagaagcu cuggucgagu ucaaagaugc acacgcuaaa     1320

agacaaaccg uagucguccu aggcucccag gaaggugcgg uccacaccgc ccucgcagga     1380

gcccucgagg cagaaaugga cggcgcuaaa ggacgccuaa gcuccggcca ccucaaaugc     1440

cgccuaaaaa uggacaaguu acgccucaaa ggagugaguu acagccucug cacagcggcc     1500

uucaccuuca caaaaauccc agcagaaaca cuacacggaa ccgucaccgu cgagguucaa     1560

uacgcaggca cggacgggcc augcaaaguc ccagcccaaa uggccgucga caugcaaacu     1620

cuaaccccag uaggccgccu cauuacagcc aacccaguaa ucacugaauc caccgagaac     1680

agcaaaauga ugcucgaacu cgacccacca uucggggacu ccuacaucgu caucggcgua     1740

ggcgagaaga aaauaacaca ccacuggcac cgcucgggga gcacucucgg caaggccuuc     1800

ucaaccacac ucaaaggcgc ccaacgacuc gccgcccuag gagacaccgc augggacuuc     1860

ggcucaaucg gcggagucuu caacagcaua ggccgcgccg uacaccaagu auucggcgga     1920

gcauuccgca cacuguucgg gggaaugucc uggauaacac aaggccucau gggcgcccua     1980

uuacuuugga uggggguaaa cgcccgagac cgaagcauag cccuagccuu ccuagccacc     2040

ggagggguac ucguauuuuu agccaccaac guccacgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  4972
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 1 opt

<400>  4972
gggagaaagc uuaccaugcg caucauaaac gcccgcaagg aaaagaaaag acgcggggcc       60

gacacauccg ucggaauagu aggccugcua cuaacaaccg ccauggccgc cgagguaaca      120

agacgcggca gcgccuauua cauguaccuc gaccgcaaug acgcaggaga agcaauauca      180

uuccccacca ccuugggaau gaacaaaugu uacauccaaa ucauggaccu aggacacaug      240

ugcgacgcaa caaugaguua cgagugccca augcuggacg aaggagugga accagacgac      300

gucgacuguu ggugcaacac caccagcacc uggguaguau acggcacaug ccaccacaag      360

aaaggcgaag cacgacgaag ccggagagcg guaacccucc ccagccacuc cacacgcaaa      420

cugcaaacca gaucccaaac cuggcuagaa agccgcgaau acacaaaaca ucucauacgc      480

guggaaaacu ggauauuuag aaacccaggc uucgcccuag ccgccgccgc aaucgcaugg      540

cuccucggaa gcuccaccag ccaaaaaguu auuuaccugg uaaugauacu guugauagca      600

cccgccuaca gcauucggug uaucggcgua agcaacagag acuucgucga aggaaugucg      660

ggaggcaccu gggucgacau cguccuagag cacggcggcu gcguaaccgu cauggcacaa      720

gacaaaccaa cagucgacau cgagcucguc accaccaccg uaucaaacau ggccgaagug      780

cgcagcuacu gcuacgaagc aagcauauca gacauggcuu cugacagcag augccccaca      840

caaggagagg ccuaccuaga caagcaaucc gacacacagu acguaugcaa acgaacccua      900

gucgaccgag gauggggaaa cggaugcgga uuauucggca agggaucccu agucacgugc      960

gcaaaauucg ccugcagcaa gaaaaugacc ggaaaaucca uacaaccaga aaaccucgaa     1020

uacaggauaa ugcucagcgu acacggcuca caacacagug gcaugaucgu caacgacacc     1080

ggacaugaaa ccgacgaaaa ccgcgccaaa guggaaauca ccccaaacag cccacgggca     1140

gaggcaacac ucggcggcuu cggcucccua ggacuggacu gcgaacccag aaccggacuc     1200

gacuucuccg accucuacua ccuaacaaug aacaacaaac auuggcuggu gcacaaagaa     1260

ugguuccacg acauaccgcu ucccuggcac gccggcgccg acacgggaac accccacugg     1320

aacaacaaag aagcccucgu agaguucaaa gacgcacacg caaaacgaca aacggucguc     1380

guccuagggu cacaagaagg cgccguccac accgcacuag ccggcgcacu cgaagcagaa     1440

auggacggcg ccaaaggacg acucucauca ggccaccuaa aaugccgccu aaaaauggac     1500

aaacuacgcc ugaaaggcgu cagcuacagc cuauguaccg ccgcauuuac guuuaccaaa     1560

auacccgcag aaacacucca cggcacaguc acagucgaag uccaauacgc cgggaccgac     1620

ggccccugca aaguaccggc ccagauggca guggacaugc aaacgcucac cccaguugga     1680

agacugauaa ccgcaaaccc cgucaucacc gagagcaccg aaaacucaaa aaugaugcua     1740

gaacucgacc cacccuucgg cgacuccuac aucgucaucg gcgucgguga aaagaaaaua     1800

acacaucauu ggcacagauc cggaagcacc aucggaaaag cauucgaagc caccguccga     1860

ggagcaaaaa gaauggcggu ccucggcgac accgcguggg acuucggaag cgugggcggc     1920

gcccucaaca gccuagggaa agggauacac caaauuuucg gugcagccuu caaaucacua     1980

uucggcggaa ugagcugguu cucccaaaua cuaaucggua cacuacucau guggcuaggc     2040

cuaaacacca aaaacggcag cauaagccua augugucucg cucuaggcgg cguacucauc     2100

uuccucucua cagcagucag cgccgacgua ggcugcagcg ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  4973
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4973
gggagaaagc uuaccaugau caucaaugcu cgaaaagaga agaaacgacg uggcgcagac       60

acaucggucg gcauaguagg acucuuacuc acaaccgcaa uggcagcgga agucacccga      120

cgcgggagcg ccuacuacau guaccucgac cgcaacgaug ccggugaggc aaucucauuc      180

cccacgacac uaggaaugaa caaaugcuac auccaaauca uggaccucgg gcacaugugc      240

gaugcgacua uguccuauga gugcccaaug cuggaugaag gagucgaacc cgacgacgua      300

gacugcuggu gcaacacgac caguacaugg gucguauacg gaacgugcca ccacaagaaa      360

ggcgaagccc gccgcagccg ucgagcagug acccuaccca gccacucaac acgcaaacuc      420

caaacacguu cacaaaccug gcucgaaagc cgagaauaca cgaaacaccu aauaagaguc      480

gaaaacugga ucuuuaggaa cccgggcuuc gcacuagcag cggccgcgau agccuggcuc      540

cuggggagcu cgaccucaca aaaagucaua uaccugguaa ugauccuacu gaucgccccg      600

gcauacucua uacgcuguau aggcgucucg aaccgcgacu ucguagaagg aaugagcgga      660

gggacauggg ucgacauagu ccuagaacac gggggcugcg uaacagucau ggcccaagac      720

aaaccaaccg ucgacaucga auuagucaca acuacagucu cgaacauggc cgaaguacgg      780

agcuacugcu acgaagccag caucagcgac auggccucag acagccgaug cccaacacaa      840

ggcgaagcau accucgacaa acaaagcgac acacaguacg uaugcaaacg cacccuagua      900

gacagaggau ggggaaacgg uugcggacuc uucgggaaag gaagccuagu cacaugcgca      960

aaauucgccu gcagcaagaa aaugacuggc aaaaguauac aaccagaaaa ccucgaauac     1020

cgaaucaugc ucagcgugca cggaucacaa cacucuggga ugauaguaaa cgacaccgga     1080

caugaaaccg augaaaaccg cgccaaagua gagaucacac caaacagccc cagggccgaa     1140

gccacccucg gaggcuucgg cagccuggga cuagacugcg aaccccgcac aggccucgac     1200

uucucagauc uauacuaccu cacaaugaac aacaaacacu ggcuagucca caaagaaugg     1260

uuccacgaca ucccucuacc guggcacgcc ggcgcagaca ccggcacccc acacuggaac     1320

aacaaagaag cccuagugga guucaaagac gcacacgcaa agcgccagac aguaguagua     1380

cuagggagcc aagaaggagc aguccacaca gcacucgccg gggcccuaga agccgaaaug     1440

gacggcgcaa agggacgccu guccucggga caccuaaaau gccgacugaa aauggacaag     1500

cuccgacuaa aaggcguaag cuacagucua ugcacagccg cguucacauu caccaaaauc     1560

ccugcagaga cgcugcacgg gaccguaaca guagaaguac aauacgccgg cacggacggg     1620

cccugcaaag uaccagcaca aauggccguc gacaugcaga cacucacacc cguaggcaga     1680

cuaaucacag caaaccccgu aaucaccgag ucgacagaaa acaguaagau gaugcuggaa     1740

cucgacccac ccuucggaga cuccuacaua guaauaggcg ugggugaaaa gaaaaucaca     1800

caccacuggc accgaagcgg aagcacaaua ggcaaagccu ucgaagcaac aguaagaggg     1860

gcaaaacgca uggccguccu cggcgacaca gcgugggacu ucggaagcgu cggaggcgca     1920

cucaacuccc ucggaaaagg aauccaccaa auauucggag cagccuucaa aucccuauuc     1980

ggcgggauga guugguucag ccaaauacug auaggcaccc ugcuaaugug gcuaggacuc     2040

aacaccaaaa acgggucaau aucacucaug ugccucgcgc uaggaggagu cuuaaucuuc     2100

cuaucaacag cugucagcgc cgauguagga ugcuccguug acuuuucaaa augaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  4974
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4974
gggagaaagc uuaccaugau caacgcaagg aaggagaaga aacgccgagg ggccgacacg       60

agcgugggca uagucggccu ccuacucaca accgcgaugg cggcagaggu aacaagaaga      120

ggcagcgccu acuacaugua ccucgaccgc aacgacgcag gagaagcaau aucauuccca      180

accacccuag gcaugaacaa auguuacaua caaaucaugg accuaggcca caugugcgac      240

gccacaaugu ccuacgaaug cccaaugcua gacgagggcg uagagcccga cgacgucgac      300

ugcuggugca acaccacuag caccugggug guauauggca cuugccauca caagaaagga      360

gaagcccgcc gcuccaggag agcaguaacc cuccccagcc acagcacaag aaaacuccaa      420

acaagauccc aaacuuggcu ggaaucgaga gaauacacca aacaucuaau aagagucgag      480

aacuggaucu uccgaaaccc aggauucgca cuagccgcag cagcaauagc cuggcuucua      540

ggcucaagca caucccagaa aguaaucuac cuagucauga uacucuugau agcaccagca      600

uacucaaucc gcugcauagg aguaagcaac cgcgacuucg ucgaaggaau gagcggaggc      660

acaugggucg auaucguacu cgagcacgga ggcugcguua cggucauggc acaagacaaa      720

cccaccguag acaucgaacu gguaaccaca acagugagca acauggcaga aguacgcagc      780

uacuguuacg aagcaucaau cucagacaug gcaucagaca gccgaugccc aacacaaggc      840

gaagcguacc ucgacaaaca aagcgacaca caauacguau guaaacggac ccuggucgac      900

cgaggauggg gcaacggaug cggacuguuc ggcaaaggau cgcucguaac augcgcaaaa      960

uucgcaugca gcaagaaaau gaccggaaaa agcauacaac ccgagaaccu cgaauaccgg     1020

aucaugcucu cgguacacgg gucccaacac agcggcauga uaguaaacga cacgggacac     1080

gaaacagacg agaacagagc gaaagucgaa auaacaccaa acagcccccg agcagaagcg     1140

acccugggcg gcuucggguc ccugggacua gacugcgaac caagaacugg acuagacuuu     1200

uccgaccuau acuaccuaac caugaacaac aaacacuggc ugguacacaa agaaugguuc     1260

caugacaucc cgcucccaug gcacgccggg gccgauaccg gcacacccca cuggaacaac     1320

aaagaagccc uagucgaauu uaaagacgcc caugccaaaa gacaaacagu cgucguacug     1380

ggcagccaag agggcgccgu acacaccgcg cucgcaggcg cacuagaagc cgaaauggac     1440

ggagcaaaag gaagacuaag cucaggccac cuaaaaugca gacucaaaau ggacaaacug     1500

cgccugaaag gagugucgua cucccuaugc acagcagccu ucaccuucac aaaaauccca     1560

gcagagacgc uacacgggac agucaccgua gaggugcaau augcagggac agacggccca     1620

ugcaaaguac ccgcccaaau ggccguagac augcaaaccc uaacaccagu cggacgccuc     1680

auaacagcaa acccaguaau aacagaaucu acggagaacu cgaaaaugau gcuggaacua     1740

gauccgcccu ucggcgacag cuacaucgua auaggagucg gugaaaagaa aauaacacac     1800

cacuggcaca gauccggcag cacgauagga aaagccuucg aggcaacagu aaggggcgcc     1860

aaacgaaugg caguacuagg cgacaccgcc ugggacuucg ggucagucgg aggggcacua     1920

aacagucucg ggaaaggcau acaccaaauu uucggagccg ccuucaaaag ccuauucgga     1980

ggaaugucgu gguucucaca aauccuuaua ggcacgcuac uaauguggcu ugggcuaaau     2040

accaaaaacg gcagcaucag ccugaugugc cuagcacucg gaggcgugcu cauauuccuc     2100

agcaccgccg ucagcgcgga cgucggaugc agcguggacu ucagcaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  4975
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4975
gggagaaagc uuaccaugaa cgcgcgaaaa gaaaagaaaa ggcgaggggc cgacacaagc       60

guuggaaucg ucggccuacu cuugacaacg gcaauggcag cagaaguaac acggcgcgga      120

ucagcguacu acauguaccu cgacagaaac gacgccggcg aagccaucag cuuccccacg      180

acccucggca ugaacaaaug cuauauacaa aucauggacc ucggccacau gugcgacgca      240

acaaugagcu acgaaugucc aaugcucgac gaaggggucg aaccagacga cguagacugu      300

uggugcaaca caacgagcac auggguagua uacggaacau gccaccacaa gaaaggcgaa      360

gccagacgca gccgaagggc aguaacacuc cccagccacu caacacgcaa acuccaaacc      420

cgaucacaaa cguggcuaga aucccgugaa uacaccaaac accuuauacg cgucgaaaac      480

uggaucuucc gcaauccagg guucgcacuc gccgcagcag ccaucgcgug gcugcuagga      540

uccagcacau cgcaaaaagu aaucuaccua guaaugaucc uccuaauugc cccggcguac      600

ucaauccgau gcauaggagu cucaaaccgg gacuucguag aaggcaugag cggaggcaca      660

uggguagaca uuguccuaga acacggcggg ugugucaccg ucauggccca agacaagcca      720

accguggaca ucgaacucgu cacaacaacc guaucuaaca uggcagaggu aagaucauac      780

ugcuacgaag ccaguauauc cgacauggca agcgauucca gaugcccaac ccaaggcgaa      840

gccuaccuag auaaacaauc agacacccaa uacguaugca aacgcaccuu agucgaccga      900

ggcuggggca auggaugcgg ccucuucggg aaaggaagcc uagucacuug ugcgaaauuc      960

gccugcagca agaagaugac aggcaaaucc auccagcccg aaaaccucga auaccgaauc     1020

augcuaagcg uacacggcag ccaacacucu ggaaugauag ucaacgacac ugggcacgaa     1080

acagacgaaa accgcgcgaa aguggaaauc accccaaacu cacccagagc cgaagccaca     1140

cuaggaggcu ucgguucacu cggacucgac ugcgaaccaa gaaccggccu ugacuucuca     1200

gaccuauauu aucuaacaau gaacaacaag cacuggcugg ugcacaaaga augguuccac     1260

gacauaccuc ucccguggca cgcaggcgca gacacaggaa caccacacug gaacaacaaa     1320

gaagcccuag ucgaguucaa agacgcacac gccaaacgac agacuguagu cgugcuagga     1380

agccaagaag gagccgugca cacugcccuc gcaggggcac uagaagcaga aauggacgga     1440

gccaaaggaa gacuaagcag cggacaccug aaaugcagac uaaaaaugga caaacuaagg     1500

cucaaaggag ucaguuacuc acuaugcaca gccgcguuca cauucacaaa aauacccgca     1560

gaaacgcucc acggaacagu cacaguugaa guccaauacg caggcacaga cggccccugc     1620

aaagugcccg cccaaauggc aguagacaug caaacacuca cgcccguagg gagacuaaua     1680

acggcaaacc cggucauaac cgaaagcacc gaaaacagca aaaugaugcu cgaacucgau     1740

ccgccguucg gcgauagcua caucgucaua ggagucggcg aaaagaaaau cacacaccac     1800

uggcacagau ccggauccac aaucggaaaa gccuucgaag ccacagugcg aggagccaaa     1860

cguauggcgg uacucggcga cacggccugg gacuucggca gcgucggggg agcacugaac     1920

agccuaggaa agggaaucca ccaaaucuuc ggagccgccu uuaaaagucu cuucgggggc     1980

augucauggu ucagucaaau acucaucgga acacuacuca uguggcuagg ccuaaacacc     2040

aaaaacggau ccauaucacu aaugugccuu gcccuaggcg gaguccuaau auuccucuca     2100

acagcaguca gcgccgacgu cgggugcucg gucgacuucu caaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4976
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4976
gggagaaagc uuaccauggc cagaaaagag aaaaagagac gaggagcgga cacguccguc       60

ggcaucgucg gucuccuucu gacaaccgcc auggccgcgg agguaacccg ccgcggaagc      120

gccuacuaua uguaccucga cagaaacgac gccggagaag caaucucauu ccccacaacc      180

cuaggaauga acaaaugcua cauccaaauc auggaccucg gccacaugug cgacgccaca      240

augagcuacg agugccccau gcuagacgaa ggaguagagc ccgacgacgu cgacugcugg      300

ugcaacacca ccucaaccug gguagucuac ggaacaugcc accacaagaa aggcgaggca      360

agacgcuccc gccgagccgu uacgcuuccg ucacacagca ccagaaaacu ccaaaccaga      420

ucccagaccu ggcucgaauc cagagaauac acuaaacacc uuauacgagu cgagaacugg      480

aucuucagga acccaggcuu cgcguuagca gcagccgcga ucgccuggcu acucggcagu      540

uccaccagcc aaaaaguaau uuaccucgug augauccuac uaaucgcacc ggcauauucu      600

auaagaugca uaggcgucag caacagggac uucguggaag gaaugucagg aggcaccugg      660

gucgauaucg uacuagaaca cggaggcugc gucaccguca uggcccaaga caaaccaacg      720

gucgacaucg aauuagucac cacaaccgua agcaacaugg cagaagucag aagcuacugc      780

uacgaagcau ccaucuccga cauggcaagc gacucacgcu gccccaccca aggcgaagca      840

uaccucgaua aacaaagcga cacacaauac guaugcaaaa ggacacucgu ggacagagga      900

ugggggaacg gcugcggccu auucggcaaa ggaagccucg ugaccugcgc caaauucgca      960

ugcuccaaga aaaugaccgg aaaaucaauc caaccagaaa aucucgaaua cagaaucaug     1020

cucuccgucc acggguccca acacagcggc augauaguua acgacacagg ccaugagacc     1080

gacgaaaacc gggcuaaagu agagauaacc cccaacucac cccgggccga agccacgcua     1140

gggggcuucg gaagccuagg acucgacugu gaaccgcgua cgggacucga cuucuccgac     1200

cuguauuauc ucacaaugaa caacaaacac uggcuaguac acaaagagug guuccacgac     1260

auaccgcuac cauggcacgc cggcgccgac accggaacac cacacuggaa uaacaaagaa     1320

gcccucguag aguucaaaga cgcgcacgca aaaagacaaa ccgugguagu cuuaggcuca     1380

caggaagggg caguacacac agcccuggca ggagcacuag aagccgaaau ggauggagca     1440

aaagggcgac uaagcagcgg gcaccuuaaa ugccgacuca aaauggacaa acuacgccuc     1500

aaaggagucu ccuacucgcu auguaccgcc gcauuuacau ucaccaaaau ccccgcagaa     1560

acccuccacg gaacaguaac aguagaaguc caauaugccg gcacagaugg cccaugcaaa     1620

guaccugcgc aaauggcagu cgacaugcaa acacuaacgc cugucggccg acuaaucaca     1680

gccaacccag uaaucacaga aucgaccgaa aacagcaaga ugaugcuaga acuagaccca     1740

cccuucggcg acuccuacau agucauaggg guaggagaaa agaaaaucac ucaccacugg     1800

cacaggagcg gcuccaccau aggaaaagcg uucgaagcua ccgucagagg cgcaaaacgc     1860

auggcgguac ugggagacac cgcaugggac uucggaagcg ucggaggggc ccuaaacucc     1920

cuaggaaaag gaauccacca aauauucgga gccgccuuca aaagccuauu cggaggcaug     1980

agcugguuuu cccaaauacu aaucggcacc cuccucaugu gguuaggacu aaacaccaaa     2040

aacggcagca uaucacucau gugccucgcg cuaggcggcg uccucauauu ccucagcaca     2100

gcagugucgg ccgacgucgg cugcagcgua gacuuuucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  4977
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4977
gggagaaagc uuaccaugag aaaagagaag aaacgaagag gagcagacac cagcguagga       60

auagucggac uccuccuaac uaccgcaaug gcagcagaag ucacgcgaag aggcagcgcg      120

uacuacaugu accuagaccg gaacgaugcc ggcgaggcca ucucauuccc aacaacacuc      180

gggaugaaca aaugcuacau acagaucaug gaccucgggc acaugugcga cgccacuaug      240

uccuacgagu gcccgaugcu ggacgaaggc gucgagccag acgacgucga cuguuggugu      300

aacacuacca gcaccugggu aguauacgga acaugccacc acaagaaagg agaagcccgc      360

aggagcagac gcgcggucac gcugccaucc cacagcacgc gcaaacucca aacuagguca      420

caaaccuggc uagagagccg agaauacacc aagcaccuaa ucagagucga aaacuggaua      480

uuccgcaacc caggauuugc acuagcggcc gccgcaauag cguggcuacu aggcucgagc      540

acaucccaaa aagugaucua ccuagucaug auacuccuaa ucgcacccgc auacagcauc      600

cgaugcauag gagucucuaa cagagauuuc gucgaaggaa ugucgggcgg aacguggguc      660

gacauaguac uagaacacgg aggcugcgua accgugaugg cccaagacaa accaaccguc      720

gacaucgagc uagucacgac uaccguaagc aacauggcag aaguacgauc cuacugcuac      780

gaagcaagca ucucagacau ggccagcgac agccgaugcc ccacccaggg cgaagcauac      840

cuagacaaac aaucagacac acaauacguc ugcaaaagaa cacucguaga ucgcggaugg      900

ggcaacggau gcggacucuu cggaaaaggc agucuaguca caugcgccaa auucgcaugc      960

ucaaagaaaa ugaccgguaa auccauacaa ccagaaaacc ucgaauaccg gauaaugcuc     1020

ucaguacaug ggagccaaca cagcggcaug auagucaacg acacagggca cgaaacugac     1080

gaaaaccgcg ccaaaguaga aauaacacca aacagccccc gcgccgaagc cacacuggga     1140

ggcuuuggaa gccucggcuu agauugcgag ccacgaacag gccuagacuu cucggaccua     1200

uacuaccuca cgaugaacaa caaacacugg cuaguacaua aagaaugguu ccaugacauc     1260

ccccucccau ggcacgcugg cgcggacacg gggacaccac acuggaacaa caaggaagcc     1320

cugguagagu ucaaggacgc ccacgcgaaa aggcaaacag ucgugguauu aggcagccag     1380

gaaggcgccg uccacaccgc auuagccggc gcccuggaag ccgaaaugga cggagcaaaa     1440

ggcagauuaa gcuccggaca ccucaaaugc cggcuaaaga uggacaaacu cagauugaaa     1500

ggaguauccu acagccuuug caccgcagcc uucaccuuca cgaaaauacc agccgaaaca     1560

cuacacggaa caguaacggu cgaagugcaa uacgcaggaa cagacggccc cugcaaagua     1620

ccagcgcaaa uggcagucga caugcaaacc cugacgccgg ucggcagacu aaucaccgca     1680

aacccaguca ucacagaauc caccgaaaac ucaaaaauga ugcuagaacu ggacccaccc     1740

uucggagacu ccuacauugu aauaggaguc ggcgaaaaga aaauaaccca ccacuggcac     1800

cgaucaggca gcacaauagg caaagcauuc gaagccaccg uaagaggcgc aaaacgcaug     1860

gcaguacugg gagacaccgc augggacuuc ggcagcguug gcggagcccu caacuccuua     1920

ggcaaaggaa uccaccaaau auucggagca gcauucaaaa gccuguuugg cgguaugagc     1980

ugguucuccc aaauacucau cgggacacug cugauguggc ucggacucaa cacgaaaaac     2040

gguagcauaa gucugaugug ccucgcccua ggcggcguac ucaucuuccu aaguacagcg     2100

guaucagcag acguaggcug cagcgucgac uucagcaaau gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  4978
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4978
gggagaaagc uuaccaugaa agaaaagaaa cgccgaggcg cagacaccag uguaggcaua       60

guaggacuuc uccuaacaac agccauggcg gcggaaguaa cucgcagagg cuccgccuac      120

uacauguauc ucgaccgaaa cgacgcgggu gaagcaauca gcuucccaac cacucucggc      180

augaacaaau gcuacaucca aaucauggac cucggccaca ugugcgacgc cacaaugagc      240

uaugaaugcc ccaugcuaga cgaaggcgua gaacccgacg acguagacug cugguguaac      300

accaccucaa ccugggucgu cuacggaacg ugccaccaua agaaaggaga agcacgccgc      360

agcagacgcg cggucacauu accaagccau agcacgcgca aacuacaaac ccggucacag      420

acauggcucg agagccgaga guacaccaaa caccuaauac gcgucgaaaa cuggauauuc      480

cgaaaccccg gauucgcacu ugcagcggcc gccaucgcau ggcuccuggg cucaagcacc      540

agccaaaaag uaaucuaccu agucaugauc cugcuaaucg ccccagccua cagcaucagg      600

ugcauaggcg ugagcaaccg agacuucguc gaaggaaugu cgggcggaac cuggguagac      660

aucguacugg aacauggcgg cugcgucacc guaauggccc aagacaagcc caccgucgac      720

auagaacucg ucacaacuac cgucuccaac auggccgagg uacgcuccua cugcuacgaa      780

gccucaaucu ccgacauggc cagcgacuca cgaugcccua cgcaaggcga agccuaccug      840

gacaaacaau cggacacaca auacgugugc aaacgaacau uagucgaccg aggauggggg      900

aacggcugcg gccuguucgg caaaggcuca uuagugaccu gcgcaaaguu ugccugcagc      960

aaaaagauga ccggaaaauc cauacaacca gaaaaccuag aauacagaau caugcuguca     1020

guacauggcu cacaacacag ugguaugauc gucaacgaca cgggccacga aacagacgaa     1080

aaccgagcga aaguagaaau caccccaaac agcccgcgcg cugaagccac acucggaggu     1140

uucggcucac uaggccuaga cugcgagcca cgaacuggcc uugacuucuc cgaccuguac     1200

uaccucacca ugaauaacaa acacuggcua guacacaaag aaugguucca cgacauccca     1260

cuacccuggc acgccggcgc cgacaccggg accccacacu ggaacaacaa agaagcacug     1320

guagaguuca aagacgccca cgccaaaaga caaaccgucg uaguacuagg cagccaagaa     1380

ggugcggucc acaccgcccu agccggcgcc cuagaagccg aaauggacgg ugccaaaggc     1440

cgccuuagcu ccggucaccu aaaaugccga cuaaaaaugg acaaacugag acuaaaaggc     1500

gucucauacu cauuaugcac agcagccuuu acuuucacaa agaucccagc cgagacgcua     1560

cacggaacag uaacaguaga aguacaguac gccgggaccg acggccccug caaagucccc     1620

gcccaaaugg ccguagacau gcagacacua accccagucg ggagacugau caccgccaac     1680

ccgguaauaa cagaaagcac cgaaaacucc aaaaugaugc uugagcuaga cccaccuuuc     1740

ggggauagcu acaucgucau cgggguaggc gagaagaaaa uaacacauca cuggcauaga     1800

agcggcagca cgauaggaaa agcauucgaa gcaacagucc gcggcgcaaa acgcauggcc     1860

guuuugggcg acacagcaug ggacuucggc uccgugggcg gcgcacucaa cuccuuaggc     1920

aaaggcaucc accagaucuu cggggccgcc uucaaaucac uauucggcgg caugucaugg     1980

uucagccaga uccucauagg cacacuccua auguggcugg gacuaaauac caaaaacggc     2040

uccaucucac ucaugugccu ugcccucgga ggcguccuaa ucuuccuuag uacagccgua     2100

agcgccgacg ucggaugcuc cguagacuuc uccaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4979
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4979
gggagaaagc uuaccaugga aaagaaaaga cgcggcgccg acacgagcgu cgguauagua       60

ggacuucuac ucacgaccgc cauggcggcc gaaguaacca gacggggcag cgccuacuac      120

auguaucucg accgcaacga cgccggcgaa gccaucucgu uccccaccac acuagggaug      180

aacaaauguu acauccaaau aauggaccuc gggcacaugu gcgacgcgac aaugaguuac      240

gaaugcccaa uguuagacga aggcguagaa ccggacgacg ucgauugcug guguaacacc      300

acaucaaccu ggguagucua cgggaccugc caccacaaaa agggcgaagc ccgaagauca      360

cggagagccg uuacccuacc gagccacucc acaagaaaau uacaaacacg aagccagacc      420

ugguuagaau caagagagua cacaaaacac cugauaagag uagagaacug gaucuuccga      480

aacccgggcu ucgcacuagc cgccgccgca aucgccuggc uccuaggauc cucaaccagc      540

caaaaaguca uuuaccucgu aaugauccuc cucauagcac cagcauacuc cauccgaugc      600

aucgguguuu ccaacagaga cuucguagaa ggcaugucgg gcggcaccug ggucgacauc      660

guccuagagc acggaggcug cgugacuguc auggcacaag acaagcccac cgucgacauc      720

gagcuaguaa ccacaacagu auccaacaug gcugaagucc gaagcuacug cuacgaagca      780

ucaaucucug acauggccag cgacagccga ugccccacac aaggagaagc cuaccuagac      840

aaacaaagcg acacccaaua cgucugcaag cgcacccuag ucgaccgcgg auggggaaac      900

gggugcggac ucuucgggaa gggaagccua guaacaugcg ccaaauucgc gugcucaaag      960

aaaaugacgg gcaagaguau ccagcccgaa aacuuagaau acagaaucau gcucucgguu     1020

cacggauccc aacacagcgg caugauagua aacgacaccg gccacgaaac agacgaaaac     1080

agggcaaaag uagaaaucac ccccaacuca cccagagccg aagccacgcu cggcggcuuc     1140

gggucacucg ggcucgacug cgagccccga accggacuug acuucagcga ccuuuacuac     1200

cuaacaauga acaacaagca cugguuagug cacaaagagu gguuccacga uaucccacuc     1260

cccuggcacg caggcgcaga caccgguaca ccccacugga acaacaaaga agcacucgua     1320

gaguucaaag augcccacgc aaaaaggcaa accgucguag uauuaggcag ccaagaaggc     1380

gcaguacaca ccgcccucgc cggagcccua gaagcagaaa uggacggcgc aaaaggaaga     1440

cucuccucag gccaccucaa augccgauug aagauggaca aacuucgacu caagggaguc     1500

aguuacucau uaugcacugc agccuucacc uucaccaaaa ucccggcaga aacgcuacac     1560

ggaaccguaa ccgucgaagu ccaauaugca ggcaccgacg gcccgugcaa aguacccgcc     1620

cagauggcgg ucgacaugca aacgcugaca ccgguagguc gccucauaac cgccaacccc     1680

guuauaacgg aauccaccga aaacuccaaa augaugcucg aacuagaccc gcccuucgga     1740

gacagcuaca ucgugaucgg ugucggagaa aagaaaauaa cccaccacug gcacagaagu     1800

gggagcacaa ucggaaaagc cuucgaagcc accgucagag gcgccaaacg cauggccguc     1860

cucggcgaca cagcauggga cuucgggagc gucggcggug cccuaaacag ccucggcaaa     1920

ggcauacacc aaauauucgg agcagcuuuc aagagcuuau ucgggggcau gagcugguuc     1980

ucccaaaucc uaaucgggac ccuacucaug uggcucggac uaaacaccaa aaacggaagc     2040

auuagccuca ugugccuggc ccucgggggc guacucaucu uccugucgac cgccguaagc     2100

gcugaugucg gaugcuccgu agacuucuca aaaugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  4980
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4980
gggagaaagc uuaccaugaa gaaacgacgg ggcgcagaca cauccgucgg caucguagga       60

cuccugcuaa ccacagcaau ggcagccgaa gucacacgca ggggcucugc auacuauaug      120

uaccuagacc ggaacgacgc aggagaagcc auaucguucc caaccacacu gggaaugaac      180

aaaugcuaua uacagauuau ggaccucggc cacaugugcg acgccaccau gagcuacgaa      240

ugcccuaugc ucgacgaggg cguggaacca gacgacgucg acugcuggug caacacgaca      300

agcaccuggg uggucuacgg aaccugccac cacaagaaag gcgaggcgag acgcucccga      360

cgcgcgguca cccucccaag ccacucaacg cgcaaacuac agacaagauc ccaaacaugg      420

cucgaaagcc gcgaauacac caaacaccua auccgagucg aaaacuggau cuuccgcaac      480

cccggauucg cacuagccgc cgccgcaaua gcauggcucu uaggcucaag caccagccaa      540

aaaguaaucu accucguaau gauccuucuc aucgcaccag ccuauucaau aagaugcaua      600

ggaguuagca acagagacuu cguagaagga augucgggag gaaccugggu cgacaucguc      660

cucgagcacg ggggcugcgu caccgucaug gcucaggaca aaccaacagu cgauaucgag      720

cuagucacca cgacaguaag caacauggca gaaguacggu ccuacugcua cgaagcaucg      780

aucuccgaca uggcaucaga cucacgaugc ccaacccaag gcgaagcaua ccuagacaag      840

caaucagaca cgcaguacgu augcaagcga acacugguag acagaggcug gggcaacgga      900

ugcggccuau ucggaaaggg cucacuggua accugugcaa aauucgcaug cucaaagaaa      960

augacaggaa aaagcauaca acccgaaaac cucgaguacc guauaaugcu cucaguccac     1020

ggaagccaac auuccggcau gaucguaaac gacacaggac acgagaccga cgaaaacaga     1080

gccaaagucg aaaucacgcc caauagccca cgcgccgaag ccacccuagg aggcuucgga     1140

ucacuaggau uggacugcga accacguacc ggccuagacu ucucagaccu auacuaccua     1200

accaugaaua acaaacacug guuagugcac aaagaguggu uccacgacau accacuccca     1260

uggcacgcgg gcgcggacac aggcacaccc cacuggaaca acaaagaagc ccuaguagag     1320

uucaaagacg cacacgcaaa acggcaaacc guaguagucc ugggcagcca agaaggcgcc     1380

guacacaccg cgcuggccgg agcccucgaa gcagaaaugg acggcgcgaa aggaagacua     1440

uccuccggcc accuaaaaug ccgacucaaa auggacaagc uaaggcugaa aggcgucagc     1500

uacucccucu guaccgcagc guuuaccuuc accaaaaucc cagcagaaac ccuccacgga     1560

acagugacag uagaagucca auacgccggc acagauggcc cuugcaaggu accagcgcaa     1620

auggcggucg acaugcaaac acuaacaccg gucggccgcc ucauaacagc caaucccgua     1680

aucacagaaa gcaccgaaaa cagcaaaaug augcucgaac uagacccgcc auucggcgac     1740

agcuacaucg ugauaggagu cggcgaaaag aaaauaacac aucauuggca cagaagcggc     1800

uccacaaucg gcaaagcauu cgaagcaaca guaagaggcg ccaaacgaau ggcaguccug     1860

ggugacacag cgugggauuu cgggucaguc ggaggugccc uuaacagccu aggaaaaggg     1920

auccaccaaa uauucggggc cgcauucaaa ucacuuuucg ggggaauguc cugguucucc     1980

caaauccuca ucggaacacu ccuaaugugg cucggacuca acaccaaaaa cggcagcauc     2040

agccucaugu gccucgcacu cggaggcgua cuaauauucc ugucaacggc aguuagcgca     2100

gacguagggu gcucagucga cuucagcaaa ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  4981
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4981
gggagaaagc uuaccaugaa gaggagaggg gcggacacca gcgucggcau agucggccua       60

cuacucacga ccgcaauggc cgccgaagua acacguagag gaucggccua cuacauguac      120

cucgacagga acgacgccgg agaagccauc ucauucccca caacacuggg aaugaacaaa      180

uguuacaucc aaaucaugga cuuaggacac augugcgacg ccaccauguc auacgaaugc      240

cccaugcucg acgaaggcgu ggaacccgac gacgucgacu gcugguguaa cacaacauca      300

accuggguag ucuacggcac augccaccac aagaaaggcg aagcccgucg cagcagaagg      360

gcaguaaccc ucccaagcca cucaacgcgg aaacugcaaa cccgaaguca aaccuggcug      420

gaaucgaggg aauacacaaa acaccuaauc cgaguagaaa acuggaucuu ccgcaaucca      480

ggcuucgcac ucgccgcagc agccauagcc uggcuacucg gcagcuccac aagccaaaaa      540

guaauuuacc ucgucaugau acuacugauc gcccccgccu acuccaucag augcaucggc      600

gucucaaacc gcgauuucgu cgaaggcaug ucagguggca ccuggguaga caucguccuc      660

gaacacggag gcugcgucac cgucauggca caagacaaac ccacagucga caucgaauug      720

gucacaacca ccgucagcaa cauggccgaa guacgaagcu acugcuacga agcgucaaua      780

uccgacaugg cgagcgacuc ccgaugucca acucaaggag aagcguaucu cgacaagcaa      840

ucggacaccc aauacguaug uaaacgaacc cuaguagacc gcggaugggg caacggaugc      900

ggccuauucg gaaaaggcuc ccucgugaca ugugcaaagu uugccugcag caagaaaaug      960

acaggcaaaa gcauccaacc agaaaaccua gaauaccgaa ucauguuguc aguacacggc     1020

ucccaacaca guggaaugau agugaacgac acaggccacg aaacagacga gaaucgcgcc     1080

aaagucgaga uaaccccuaa cucaccacgc gcagaagcga cacugggagg auucgggucc     1140

cuaggccucg acugcgaacc acgcaccggc uuagacuuca gcgaucucua cuaccuaacc     1200

augaacaaca aacacuggcu cguacacaaa gaaugguucc acgauauccc acuccccugg     1260

cacgccggcg cugacacagg cacgccccac uggaacaaca aagaagcgcu uguggaguuc     1320

aaggacgccc acgcaaaaag gcaaaccguc guaguacuag gcucacaaga gggcgccgua     1380

cacaccgccc uggcaggcgc acucgaagcc gaaauggacg gcgccaaagg acggcucucc     1440

agcggacacc ucaagugccg ccuaaaaaug gacaaacuga gacuaaaagg cguaucauac     1500

agccugugca ccgccgcauu caccuucacc aaaauacccg cggaaacccu acacggcaca     1560

guaaccguag aaguacaaua cgcaggcacc gacggccccu gcaaaguacc ggcccagaug     1620

gccguagaca ugcaaacccu cacucccguc ggacgccuca ucacagcaaa ccccgucaua     1680

acagaaucca ccgaaaacag caaaaugaug cucgaacucg acccacccuu cggagacucc     1740

uacauaguaa ucggagucgg cgagaagaaa aucacccacc acuggcaucg aucaggauca     1800

acgaucggca aagcauucga agcaaccgug cggggagcaa aaaggauggc cguacucggc     1860

gacaccgccu gggacuucgg gucagucggg ggcgcccuaa acucccuggg aaaaggcauu     1920

caccaaaucu ucggugccgc cuucaaaagc cuguucgggg gcaugagcug guucagccaa     1980

auacucauag gcacacuccu gauguggcua ggccucaaca ccaagaacgg cagcaucagu     2040

uuaaugugcc uagcccuggg aggaguccuc aucuuccuca gcacagccgu aucagcagac     2100

guaggcugua gcgucgacuu cagcaaauga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4982
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4982
gggagaaagc uuaccaugcg ccggggcgca gacacaucag ugggcaucgu aggccuccug       60

cucaccaccg cgauggccgc agaggucacc cgacgcggga gcgccuacua cauguaccua      120

gaccgaaacg augccggcga agccaucucg uucccgacaa cccugggcau gaauaaaugc      180

uacauccaaa uaauggaccu aggucacaug ugcgacgcaa cuaugucuua cgaaugcccg      240

augcucgacg agggugugga accugaugac guagacugcu ggugcaacac cacaagcaca      300

ugggugguau acggaacaug ccaccacaag aaaggcgaag cacgacggag ccggcgcgca      360

gucacccuac cgagccacuc aacccgaaaa cuccaaaccc gcagccaaac augguuggaa      420

agccgcgaau acacaaaaca ccuaauccgc gucgaaaacu ggaucuuccg caacccgggc      480

uucgcccucg cagccgcagc caucgccugg cuuuuaggcu cauccaccuc acaaaaagua      540

aucuaccuag ucaugauccu acucauagcc ccugcauacu ccaucaggug cauaggcgua      600

uccaacaggg acuucgucga aggcaugagc ggcggcaccu gggucgacau cguacucgaa      660

cacgggggcu gcguaacagu cauggcacaa gacaaaccca cagucgacau cgaacucgug      720

acaaccacag ucagcaacau ggcagaagua agaucauacu gcuacgaagc auccauaagc      780

gacauggcau cugacagcag augcccaaca cagggagaag cauaccucga caaacaaagc      840

gacacacaau acgucuguaa acgaacccua guagaccgcg gauggggaaa cggcugcgga      900

cucuucggaa aaggcagccu cguaacaugc gccaaauucg ccugcagcaa gaaaaugacc      960

ggcaaaucca uacaacccga aaaccucgag uacagaauca ugcuuucagu ccacggaagc     1020

caacauuccg gcaugauagu caacgacacc ggacacgaaa ccgacgaaaa cagagcgaaa     1080

gucgaaauca cacccaacuc acccagggcg gaagcaacac ugggaggauu cgggucacuc     1140

ggucuugauu gugaaccccg aacuggacuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaaac acuggcuagu ccacaaagag ugguuccacg acaucccacu acccuggcac     1260

gcaggagccg acacaggcac cccacacugg aacaacaaag aagcacuggu cgaguucaaa     1320

gacgcacacg ccaaacgcca aacugucguc guacuaggca gccaggaagg agcaguccac     1380

acagcacugg ccggagcucu agaagcagag auggacggcg cgaaaggacg ccuaagcuca     1440

ggccacuuaa aaugccgacu caaaauggac aaacuccguc uuaaaggagu cucguacuca     1500

cugugcaccg cugcauucac cuuuaccaag auccccgcgg aaacccuaca cggaaccguc     1560

acaguagaag uccaauacgc cggaacugac ggcccaugca aaguccccgc ccaaauggca     1620

guggauaugc aaacccuaac ccccguagga cgccuaauaa cagccaaccc cguuaucacc     1680

gaauccaccg aaaacucuaa aaugaugcug gaacuagauc cacccuucgg agacuccuau     1740

auaguaaucg gcgucggaga aaagaaaauc acgcaccacu ggcaccggag cggcucaacc     1800

aucggaaaag ccuuugaggc gaccguccgc ggagccaaac gcauggcagu acucggagac     1860

acagccuggg acuucgggag cgucggaggc gcccuaaacu cacucggcaa aggcauccac     1920

caaaucuucg gagccgcauu caaaucccuc uucggcggca ugagcugguu cucacagaua     1980

cuaauaggaa cccuccuaau guggcucggc cucaacacca aaaacgguag caucagccua     2040

augugccuag cacuaggcgg cguacuaaua uuccucucca ccgccgucag cgccgacgug     2100

ggcuguuccg ucgacuucuc caaaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4983
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4983
gggagaaagc uuaccaugag aggcgccgau acuagcguag gaaucguggg acuccuacuc       60

acaacugcaa uggccgcaga agucacaaga cgaggaagcg cauacuacau guaucuagac      120

cggaacgacg ccggagaagc aaucagcuuc ccgacaacac uaggaaugaa uaaaugcuau      180

auccaaauaa uggaccuagg ccacaugugc gacgcgacca ugagcuacga gugccccaug      240

cucgaugaag gcgucgaacc agacgacguc gacugcuggu gcaacacuac aagcacaugg      300

guaguauacg ggacauguca ccacaagaaa ggagaagcca gacgcucgcg ccgcgcaguc      360

acacuccccu cacacucaac acggaaacua caaacgcgcu cgcaaaccug guuggaaucc      420

cgagaauaca ccaaacaccu aauccgcguc gaaaacugga ucuuccgcaa cccggguuuc      480

gcacucgcag ccgcagccau cgccuggcua cucggcagcu caacaagcca aaaagucaua      540

uaccuaguaa ugauacuacu gaucgcacca gccuacagca ucagaugcau cggcgucuca      600

aaccgcgacu ucgucgaagg aauguccggc ggaaccuggg uagacaucgu acuagaacac      660

ggcggaugcg ucaccgucau ggcacaagac aaacccacag uagacauuga gcucguaaca      720

acaacagucu ccaacauggc cgagguccga ucauacugcu acgaagcauc caucagcgac      780

auggccagcg acuccaggug cccgacccaa ggcgaagccu aucucgacaa acagagcgac      840

acgcaguacg ucugcaaacg aacauuaguc gaccgaggcu ggggaaacgg augcggccua      900

uucggaaaag gaagccuagu cacgugcgca aaauucgcau gcucaaagaa aaugaccgga      960

aaaucgaucc aacccgaaaa ccuggaauac cgaauaaugc ucuccguaca cgggagccag     1020

cacucaggaa ugauagucaa ugacaccggc cacgaaacag acgagaaucg agcuaaagua     1080

gagauaacac ccaacagccc cagagcagaa gcgacccucg gcggcuucgg cucccucggc     1140

uuagacugcg aaccacgcac cggccucgac uucagcgacc uauacuauuu aacaaugaac     1200

aacaaacacu ggcuagugca caaagaaugg uuccacgaca uaccacuacc auggcacgca     1260

ggggcagaca cggggacccc acacuggaac aacaaagaag cccuaguaga guucaaagac     1320

gcacacgcaa aacgacaaac aguagucgua cuugggagcc aggaaggcgc aguccacacc     1380

gcacuagccg gggcccucga agcagaaaug gacggggcaa aaggcagacu aagcagcgga     1440

caucugaaau gcagacucaa aauggacaaa cuccgccuaa aaggcguguc cuacucacua     1500

ugcaccgcag ccuucaccuu cacgaaaauc ccagcggaaa ccuuacacgg caccgucaca     1560

guggaaguac aauacgccgg caccgacgga ccaugcaaag uaccagcaca gauggccguc     1620

gauaugcaaa cacugacgcc cgucggacga cugaucacug caaacccggu aaucaccgaa     1680

agcacagaaa acucaaaaau gaugcucgag cucgacccuc ccuucggaga cagcuacauc     1740

gucaucggag ucggagaaaa gaaaauaacu caucacuggc accgcagcgg aucaaccauc     1800

ggcaaagccu ucgaagcuac agucagaggc gcaaagagaa uggcgguacu aggcgacaca     1860

gcaugggacu ucggaucagu gggaggcgca cugaacagcc ucggaaaggg gauacaccaa     1920

aucuucggcg ccgcauucaa aagccucuuc ggcggcauga gcugguuuuc ccagauacua     1980

aucggaacgc uccuaaugug gcuagggcua aacaccaaaa acggcagcau aagccugaug     2040

ugccucgcgc ucggaggcgu ccucaucuuc cuaucaaccg cagucucggc agauguaggc     2100

ugcucagucg acuucagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4984
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4984
gggagaaagc uuaccauggg ggccgacacu uccguaggca ucgucggacu acuccugacc       60

acggccaugg ccgccgaagu aacccgccgc ggcagcgcau acuacaugua ccucgaccgc      120

aacgacgccg gggaagcuau aucguuccca acaacccucg gaaugaacaa augcuacaua      180

caaaucaugg accucggcca caugugcgac gcaaccauga gcuacgaaug uccaaugcug      240

gacgaagggg uagaaccaga cgacguggac ugcuggugca acaccacaag caccuggguc      300

gucuacggaa ccugccacca caagaaagga gaggcccgac gaagucgccg cgcagucacc      360

cucccaagcc acucuacacg aaaacuucaa acccggucac aaacauggcu cgaaucaaga      420

gaauacacca aacaccucau ccgagucgaa aacuggaucu ucagaaaccc cggauucgcc      480

cucgcagcgg ccgccauagc guggcuacua ggcucaagca ccucacaaaa agucaucuac      540

cuggucauga uccuccuaau agcacccgcc uacagcaucc gcugcaucgg cgucucaaac      600

agagacuucg uagaaggaau gagcggagga acauggguag acauagugcu cgaacaugga      660

ggcugcguaa ccgucauggc ccaagacaaa ccaaccgucg acauagaacu ggucacuaca      720

accguaucua acauggccga gguaagauca uacugcuacg aagccuccau cuccgacaug      780

gccagugaca gccgaugccc aacgcaaggc gaagccuauc ucgacaaaca auccgacacc      840

caauaugucu gcaagcgcac acucguagac cgcggcuggg gaaacggcug cggccucuuc      900

ggcaaaggca gccuaguaac cugcgcaaaa uucgcaugcu ccaagaaaau gacaggaaaa      960

uccauccaac ccgaaaaccu cgaauaccgc aucaugcucu ccgugcacgg cagccaacac     1020

uccggaauga ucguaaacga caccggacac gaaacagacg aaaaccgcgc caaagucgaa     1080

auaacaccaa acuccccacg cgcggaagcc acacuaggcg gcuucggcuc ccuaggccuc     1140

gacugcgaac ccagaacagg acucgacuuc agcgaccucu auuaccuaac caugaacaac     1200

aaacauuggu uaguacacaa ggaaugguuu cacgacaucc cccuccccug gcacgcaggc     1260

gcagauacag gaacaccaca cuggaacaac aaagaagccc ucguagaauu uaaagacgcc     1320

cacgcuaaac ggcaaacagu aguaguccua ggcagccaag agggggccgu acacaccgca     1380

cucgcaggcg cccucgaagc ugaaauggau ggcgccaaag gcaggcucuc aucgggucac     1440

cucaaaugcc gacuaaaaau ggacaaacua cgucuaaaag gggucucuua cucacucugu     1500

acggcggccu ucaccuucac caaaauacca gcagagacac uacacggaac cgucacaguc     1560

gagguccaau acgccggcac agacggacca ugcaaagucc cugcacaaau ggccgucgac     1620

augcaaaccc ucacaccagu aggacgccuc aucacggcca accccgucau cacagaaagc     1680

accgaaaacu ccaaaaugau gcucgaacua gaucccccgu ucggagacag cuacaucgua     1740

auaggcgucg gagagaagaa aauaacacac cacuggcacc gauccggauc cacaauaggc     1800

aaagccuucg aagcaaccgu cagaggcgca aaacgaaugg ccguacuggg agacacagcc     1860

ugggacuucg gaagcguggg cggagcacuc aacagccuug gcaaaggaau ccaccaaauc     1920

uucggcgcag cauucaaauc acucuucgga ggcauguccu gguucucaca aauacugaua     1980

gggacacucc ugaugugguu aggucucaac accaaaaacg gcagcauuuc ucucaugugc     2040

cucgcccucg gaggaguacu caucuuccuc uccacagcgg ucagcgcaga cgucggaugc     2100

uccgucgacu uuucgaaaug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4985
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4985
gggagaaagc uuaccauggc cgacaccagc gucggaaucg ucgggcuccu auugaccacc       60

gccauggccg ccgaagucac ccgccgcgga agcgccuacu acauguaccu agaccgaaac      120

gacgcaggcg aagccaucag cuuccccaca acacuaggca ugaacaaaug cuacauucaa      180

aucauggacc uaggccacau gugcgacgca accaugucau acgagugucc caugcuagac      240

gaaggaguag aaccagacga uguagacugc uggugcaaca ccaccuccac augggucguc      300

uacggaaccu gccaccacaa aaagggcgag gcacgacgau cacgccgcgc cgucacucua      360

ccaucacacu caacucgaaa acuacaaacc cgcucccaaa caugguuaga aagccgcgag      420

uauacgaaac accuaauccg aguagaaaau uggauauucc ggaacccagg cuucgcacug      480

gcagccgccg caaucgcaug gcuucuggga uccucaacgu cacaaaaagu caucuaccuc      540

gucaugauac ugcucauagc accagcauau uccauccgcu guaucggggu auccaacagg      600

gacuucguug aaggcauguc aggagggaca ugggucgaca ucguccucga acacggaggc      660

ugcgucacag ucauggcaca agacaaaccc accguagaca ucgagcucgu caccacaaca      720

gucagcaaca uggccgaagu ccgcagcuac ugcuacgaag caucaaucag cgauauggcc      780

agcgacucaa ggugcccuac ucaaggcgag gcuuaccuag auaaacaaag cgacacccaa      840

uacgucugca aacgcacccu aguagaccga ggauggggaa acggaugcgg ccucuucggc      900

aaaggcuccc ucgugacaug cgcaaaauuc gcaugcagca agaaaaugac aggcaaaagc      960

auacaaccag aaaaccucga auacagaauc augcugagcg uccacggguc acaacacuca     1020

ggcaugauag ugaacgacac cggccacgaa accgacgaaa accgcgcaaa aguagaaaua     1080

accccaaaca gcccacgagc cgaagccacc cuaggcggcu uuggaagccu cggccuagac     1140

ugcgagcccc gcaccggucu agacuucagc gaccucuacu accugaccau gaacaacaaa     1200

cauuggcucg ugcacaaaga gugguuccac gacauacccc uaccuuggca cgcaggagca     1260

gacaccggca caccccacug gaacaacaag gaggcacugg uagaguucaa ggacgcccac     1320

gcaaaacgac aaacaguagu cgugcuagga ucucaagaag gcgccgucca cacagccuua     1380

gccggggcac ucgaagcaga gauggacgga gccaaaggac gacuauccuc cggccaucuc     1440

aaaugccgcc ucaaaaugga caagcuccga cuaaaaggcg uaagcuacag ccuuugcacg     1500

gcagcauuca cguucacgaa aauucccgcc gaaacacucc acggcacagu aaccgucgaa     1560

guacaauacg ccggcacaga ugggcccugc aagguccccg cccaaauggc aguugacaug     1620

caaacccuca cccccguagg ccggcugauc accgccaacc cagucaucac cgaauccacc     1680

gagaacagca aaaugaugcu cgaacucgau cccccuuucg gagacuccua cauagucauc     1740

ggaguaggag aaaagaaaau cacccaccac uggcaccgca gcggcuccac cauaggaaaa     1800

gcauucgaag cuaccguccg cggggccaaa cgcauggccg uacucggcga cacggcgugg     1860

gacuucggcu cgguuggagg agcccuaaau agccucggca aaggaaucca ccaaauauuc     1920

ggagcggcuu ucaaaucccu uuucgguggc augucauggu uuagccaaau ucucaucgga     1980

acacugcuca uguggcuggg cuuaaacacc aaaaauggau caaucucgcu caugugccua     2040

gcacuaggag ggguccuaau auuccuguca accgcaguga gcgccgacgu aggcugcagc     2100

gucgacuucu caaaaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  4986
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4986
gggagaaagc uuaccaugga cacaucaguu ggcaucgucg ggcugcuacu caccacggcc       60

auggccgcag aagugacaag aagaggaagc gcauacuaca uguaucugga ucgcaacgac      120

gccggcgaag cgaucagcuu cccaacgacc cucggaauga acaaaugcua cauacagauc      180

auggaccuag gacacaugug cgacgcgacc auguccuacg agugcccaau gcuagacgaa      240

ggcgucgagc cagacgacgu cgacugcugg uguaauacga ccucaaccug gguagucuac      300

gguacaugcc accacaagaa aggcgaagca cgaagaucaa gacgagccgu cacccuaccc      360

ucacacagca cuagaaaacu ccaaacccga agucaaacau ggcuagaaag ucgagaauac      420

acgaaacacc ugauccgcgu agaaaacugg auauuccgaa acccaggcuu cgcgcucgcc      480

gcagccgcca uagccuggcu ccucggcagc agcaccaguc agaaggucau auaucucgug      540

augauccucc ucaucgcccc ugccuacuca auaagaugca ucggcguaag caaccgggac      600

uucguggaag gaaugucugg aggcaccugg guagacauag uccucgaaca cggaggcugc      660

guaacuguaa uggcccaaga caaaccuaca gucgacauug aacuggucac caccaccgua      720

uccaacaugg ccgaagugcg cagcuacugc uacgaagcca gcaucucaga cauggcgagu      780

gacagccgau gccccacuca aggcgaagcc uaccuagaca aacaaagcga cacacaauac      840

gucugcaaac gaacacucgu cgaucgugga ugggguaacg gaugcggccu cuucggcaaa      900

gguucccuag ucacuugcgc aaaauucgcc ugcagcaaaa agaugacagg aaagagcaua      960

caacccgaaa accuagaaua ccgcaucaug cugagcgucc acggcagcca gcacagcgga     1020

augauaguaa acgacacagg acacgaaacc gacgaaaaua gggcgaaagu cgaaaucacc     1080

cccaacagcc ccagagcgga agcaacacua gguggcuucg ggagccucgg auuagacugc     1140

gagccccgaa caggccucga cuucagcgac cuauacuacc uaacaaugaa caacaaacac     1200

ugguuagucc acaaagagug guuccaugac aucccccucc cauggcacgc cggcgccgac     1260

accggcacac cccacuggaa caacaaggag gccuuagucg aguucaaaga cgcccacgca     1320

aaacgacaaa cagugguagu ccuaggaagc caggaaggag ccguccacac ggcccucgca     1380

ggagcacucg aggccgaaau ggacggcgca aaaggccgcc ugagcucagg acaccucaaa     1440

ugccgccuaa aaauggacaa acuaagacuc aaaggaguaa gcuacucccu uugcacagca     1500

gccuucacau ucacgaagau cccagcagaa acacugcaug gcacagugac aguagaagua     1560

caauacgccg gcaccgacgg accaugcaaa gugcccgccc agauggccgu agauaugcag     1620

acucuaacuc ccguaggcag acuaaucacc gcaaaccccg ucaucaccga gagcacagaa     1680

aacagcaaaa ugaugcuaga gcucgauccc ccauucggag acaguuacau aguaaucgga     1740

guaggcgaga agaaaaucac ccaccacugg caccgcagug gauccacuau aggcaaagcc     1800

uuugaagcaa caguccgcgg cgcaaaaagg auggcagucc uaggagacac cgcaugggau     1860

uucggcagcg uaggcggagc acuaaacagc cucggcaaag gcauacacca aauauucgga     1920

gccgccuuca agagccuauu cggcggcaug uccugguuca gccaaauacu aaucggcacc     1980

cuccucaugu ggcucggacu gaacaccaaa aacgggucaa ucagccucau gugccuggca     2040

cugggagggg uacucauauu ccuaagcacc gcaguaucag ccgacguagg uugcagcgua     2100

gacuuuagca aaugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  4987
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4987
gggagaaagc uuaccaugac cucagucggc auaguaggac ugcuccugac aacagcgaug       60

gccgccgagg uaacucgaag aggaagcgcg uacuacaugu accucgaccg aaacgacgcg      120

ggcgaagcaa ucagcuuccc gaccacccua ggcaugaaca aaugcuacau ccaaauaaug      180

gaucugggac acaugugcga cgcaaccaug ucauacgaau gucccaugcu cgacgaagga      240

guagaacccg acgacgugga uugcuggugc aacacaacca gcaccugggu agucuaugga      300

acaugccacc acaagaaagg cgaagcccga cgauccagac gugccgucac gcuacccagc      360

cacagcacac gcaaacucca aaccagaagc caaacauggc ucgaaagccg agaauacacc      420

aaacaccuca uaagagucga aaacuggaua uuccgcaacc ccggguucgc acuagcugca      480

gcagccaucg cauggcuccu aggcuccagc acaagccaaa aagucauaua ccuugucaug      540

auccuacuca uagcaccggc cuacuccaua cgcugcauag gcgucuccaa ccgagacuuc      600

gucgaaggaa ugagcggagg caccugggua gauaucgucc uggagcacgg cggaugcgua      660

accgucaugg cgcaagacaa gccaaccgua gacaucgagc ucguaaccac cacgguuagc      720

aacauggccg aaguccgcuc cuacugcuac gaagcaagca uaagcgacau ggcaagugac      780

agcagaugcc ccacucaagg cgaagcguac cucgacaaac aaagcgacac acaauacgua      840

ugcaaacgaa cccucguaga cagaggcugg ggcaacggcu gcggccuauu cggaaagggc      900

ucccucguaa ccugcgcgaa guucgccugc ucaaagaaaa ugacaggcaa aucgauacaa      960

ccggaaaacc ucgaauacag aauaaugcug uccguccacg gaucacaaca cagcggaaug     1020

auagugaaug acacaggaca ugaaaccgac gagaaccgag caaaagucga aauaacacca     1080

aacuccccac gggccgaagc cacccucgga gguuuuggca gcuuaggccu cgacugcgag     1140

ccacgaacgg gccucgacuu cucagaccuc uacuaccuaa cgaugaacaa caaacacugg     1200

cuuguccaua aagaaugguu ccacgacaua ccccuccccu ggcacgccgg agccgacacc     1260

ggcacacccc acuggaacaa caaagaggcg cuaguagagu ucaaagacgc ccacgccaag     1320

agacaaaccg ugguaguacu agggagccaa gaaggcgcgg uacacacagc acuagccgga     1380

gcccucgaag ccgaaaugga uggagcaaaa gggcgacuca gcuccggcca ccucaaaugc     1440

cgccuaaaaa uggacaaacu aagacugaag ggcguaagcu acagccuaug cacagccgca     1500

uucaccuuca ccaaaauacc cgccgaaacc cuacacggaa cagucaccgu cgaaguccaa     1560

uacgccggua ccgacggacc gugcaaggua ccggcacaaa uggcaguaga caugcaaacu     1620

cuaacccccg uaggcagacu caucacggcc aaccccguaa uaaccgaauc uacagaaaac     1680

aguaagauga ugcucgaacu cgacccaccc uuuggcgacu ccuacaucgu cauaggaguc     1740

ggagaaaaga aaaucaccca ccacuggcac aggaguggca gcacaaucgg uaaagccuuc     1800

gaagccacag uacgcggggc aaaacgcaug gcgguccucg gcgacacagc augggacuuc     1860

gggagcguag gcggcgcgcu aaacagccuc ggcaaaggca uccaccagau cuucggcgcg     1920

gcauucaaau cacuauuugg agggaugagc ugguucucuc aaauccucau aggaaccuug     1980

cugauguggc ugggccuaaa cacgaaaaac gggagcauca gccucaugug ccuugcccua     2040

gguggcgucc uaaucuuccu uagcacagcc gucucagccg acgucggaug cagcguggac     2100

uucaguaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4988
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4988
gggagaaagc uuaccaugac cagcgucggu auaguaggcc uacuacuaac aacggccaug       60

gcagccgccg ugacacgcag aggaucugcc uacuacaugu accuagaccg caacgacgcc      120

ggcgaagcga ucuccuuccc uacuacccug ggcaugaaca aaugcuacau ccaaaucaug      180

gaccuaggac acaugugcga ugccacaaug ucauacgaau gcccuaugcu ggacgaggga      240

gucgaacccg acgacguaga uuguuggugc aacaccacaa gcacaugggu aguauaugga      300

acaugccacc acaaaaaggg cgaagcgcgc agaagccgcc gcgccgucac ccuacccagc      360

cacuccacaa gaaaacugca aacgagaagc caaaccuggc ucgaaucccg cgaauacacc      420

aaacaccuca uccgcgucga aaacuggauc uuccgaaacc ccggcuucgc gcuagccgcg      480

gccgcuaucg cguggcuccu gggaucaagu accagccaaa aaguaaucua ccuaguaaug      540

auccuccuca ucgcaccagc cuacuccauc cgaugcauug gcguaagcaa cagagacuuc      600

gucgagggca ugaguggcgg caccugggua gacaucgugc uagaacacgg aggcugcguc      660

accguaaugg cccaagacaa acccacaguc gacauagaac ucguaacaac aacgguuuca      720

aacauggcag aggugagauc guauugcuac gaagccagca ucagcgauau ggccagcgac      780

agccgcugcc cgacacaagg agaagcguac cuggacaaac aaagcgacac ccaauacguc      840

ugcaagcgaa cacucguaga ccgcgggugg ggcaacggcu gcggacuauu cggaaaagga      900

agccuuguaa ccugcgcaaa guucgccugc agcaagaaaa ugacaggcaa aagcauccaa      960

ccagaaaacc ucgaauaccg gaucaugcuc agcguccaug gcucacaaca cagcggcaug     1020

auagucaacg acacgggcca cgaaacagac gaaaaccgag caaaaguaga aauaaccccc     1080

aacuccccaa gagcggaagc cacccuaggc ggauucggau cccucggacu cgacugcgaa     1140

ccacgaaccg gacucgacuu cucagaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacaua ccacucccau ggcacgccgg cgccgacaca     1260

ggcacaccac acuggaacaa caaagaggcc cuugucgagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg ucguaguccu cggaucccaa gaaggagcgg uccacaccgc ccucgcaggc     1380

gcccuagaag ccgaaaugga cggagcgaaa ggacgccuau caucggggca ccucaagugc     1440

agacucaaaa uggacaaacu ccgacuaaaa ggcgucagcu acucacucug caccgccgca     1500

uucacguuca ccaaaauccc cgccgaaacc cuacacggaa cagugacagu agaagugcag     1560

uacgcaggaa cagacggacc augcaaaguc cccgcccaga uggcaguaga caugcaaaca     1620

cucacaccag ugggacgccu aaucaccgcc aaccccguaa ucacagaaag caccgaaaac     1680

agcaaaauga ugcuagaacu cgacccaccc uucggggacu ccuacaucgu aauaggaguc     1740

ggggaaaaga aaaucacgca ccacuggcac cgaagcgguu ccacaaucgg caaagccuuc     1800

gaagcaacag uacgcggcgc aaaacggaug gcgguccucg gagacaccgc augggacuuc     1860

ggaagcgucg gaggcgcccu aaacagucua ggcaaaggaa uacaccaaau auucggugcg     1920

gccuucaaaa gucuauucgg cggcaugagc ugguucagcc aaauccucau aggaacacuc     1980

uuaauguggc ucggccuaaa uacuaaaaac ggcucaauua gccuaaugug cuuagcacua     2040

ggaggcgugc ucaucuuccu gucaaccgcc gucuccgccg acguaggcug cagcguagac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  4989
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4989
gggagaaagc uuaccaugcg aggcgcagac acgagcguag gcaucgucgg ccugcuuuua       60

accacagcaa uggcugcagc cguaacccga cgaggaagcg cauauuacau guaccuagac      120

agaaaugacg caggagaagc aauaucguuc cccacaacac uaggaaugaa uaagugcuac      180

auccaaauca uggaccucgg acacaugugc gacgccacga ugucguacga augccccaug      240

cuagacgaag gagucgaacc agacgacgug gauugcuggu guaacacaac cucaaccugg      300

gugguauacg gcacgugcca ccacaagaaa ggcgaagcaa gacgaucaag acgagcagua      360

acucuccccu cccacagcac cagaaaacuc cagacaagau cccaaacaug gcuagaauca      420

cgagaauaca caaaacaccu gauaagaguc gaaaacugga uauucagaaa ccccggauuc      480

gcacucgccg cagcagccau agcauggcuc uuaggcagcu caacaagcca aaaaguaauc      540

uaccuaguca ugauccuacu aaucgcgccc gcguacucga uccgcugcau cggcguaucg      600

aacagagacu ucgucgaagg cauguccgga gggacguggg ucgacauagu gcuagaacac      660

ggcggaugcg ucacagucau ggcacaagac aaaccgacgg ucgacauaga gcuaguaacc      720

accacaguaa gcaacauggc agaagucaga aguuacugcu acgaagcauc cauaucagau      780

auggcaucag acuccagaug cccgacccag ggagaggccu accuagacaa acaaucagau      840

acccaauacg ucugcaaacg aacccuggua gaccgcggcu ggggaaacgg cugcggccug      900

uucggcaagg ggagcuuagu aaccugcgca aaauucgcau gcagcaagaa aaugaccgga      960

aaaagcaucc aacccgaaaa ccucgaauau cgcauaaugc ucagcgucca cggcagccaa     1020

cacagcggaa ugauagucaa cgacacaggc cacgaaacag acgaaaacag ggcaaaaguc     1080

gaaaucacac caaauagccc aagggccgaa gcaacacucg ggggcuucgg gucacuuggc     1140

cuagacugcg aacccagaac gggacuagac uucagcgacc uguacuaccu caccaugaac     1200

aacaaacacu ggcuaguaca caaagaaugg uuccacgaca uaccccuccc auggcacgca     1260

ggagccgaca ccgggacgcc acacuggaac aacaaggaag cacucgucga guucaaagac     1320

gcccacgcaa agcgccaaac aguagucgua cugggcuccc aagaaggggc aguacacacg     1380

gcacucgcag gcgcacucga agcagaaaug gacggcgcca aaggccgccu cucaagcgga     1440

caccucaaau gccgccucaa gauggacaag cuccgacuca aaggcguaag cuacagccuu     1500

ugcacagcag cauucacauu cacaaaaaua cccgcagaaa cacuccacgg cacagucaca     1560

gucgaagugc aauacgcagg aaccgacggc ccaugcaagg uaccagcaca aauggcaguc     1620

gacaugcaaa cgcuaacccc agucggccgc cucauaaccg caaacccagu aauaacugag     1680

agcacagaaa acagcaagau gaugcucgag cucgacccac ccuucggcga uagcuacaua     1740

gugauaggag ucggcgagaa aaagaucacg caccauuggc acagaagcgg aagcacaaua     1800

ggaaaagcgu ucgaagcaac agucagaggc gcaaaacgca uggccguccu cggcgacaca     1860

gcaugggacu ucggcucagu aggaggggca cucaauagcc uaggcaaagg aauacaccag     1920

auauucggag cggcauucaa aagccuguuc ggcgguauga gcugguucag ccagauacua     1980

auuggaaccc uacuaaugug gcuaggccua aacaccaaaa acggaagcau cagccuaaug     2040

ugccuagcac uuggcggagu acucauauuc cucagcacag cagucuccgc cgacguagga     2100

ugcagcgucg acuucagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4990
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4990
gggagaaagc uuaccaugcg agggcucuca ucacgcaaac gccgcaguca cgacguccua       60

accguccaau uucuaauccu aggaaugcuc cuaaugaccg gaggggucac ccgcagaggc      120

ucggccuacu acauguaccu cgacaggaac gacgccggcg aagcaauuag cuuccccaca      180

acccuuggua ugaacaaaug cuacauacaa auaauggacc uaggacauau gugcgacgca      240

acgauguccu acgagugucc cauguuggac gaaggcguag aaccagacga cguggacugc      300

uggugcaaca ccacaagcac auggguugua uacggcacau gccaccacaa gaaaggagaa      360

gcccgccgau cacgacgugc cgucacccug cccagccaca gcacgcgcaa acuacaaacu      420

cgcucgcaaa ccuggcugga aagccgcgaa uacacaaaac accucauccg agucgagaac      480

uggauauucc gcaaccccgg cuucgcacuc gcggcagccg caauagcaug gcuucuaggg      540

ucaagcacca gccaaaaagu caucuaccuc guaaugaucc uccugaucgc ccccgccuac      600

uccaucagau gcaucggagu aucaaacaga gauuucguag aaggcauguc agguggaacc      660

uggguagaca ucguacuaga gcacggaggc ugcgucaccg ucauggcaca ggauaaaccc      720

acagucgaca ucgaacuggu cacgaccacu gucucaaaca uggccgaagu gcguagcuac      780

uguuacgaag ccagcaucag cgacauggcg agcgacaguc gaugccccac ccaaggggaa      840

gcauaccucg acaaacaaag cgacacacaa uacguaugca agcgaacacu cguagaccgc      900

ggcuggggga acggaugcgg acucuucgga aaaggcagcc uagugaccug cgccaaauuc      960

gcuuguucaa agaaaaugac cggcaaaagc auacaaccgg aaaaccucga auacagaaua     1020

augcucuccg ugcacggauc ucagcacagc ggcaugauag ucaacgacac cggccacgag     1080

accgacgaaa acagggcgaa agucgaaaua acaccgaaca gcccccgagc cgaggcgaca     1140

cucggcggcu ucgggucccu cggccuagau ugcgaacccc gcacaggccu cgacuucagc     1200

gaccucuauu aucuaacaau gaacaacaaa cacuggcuag uccacaaaga augguuccac     1260

gacauuccac uccccuggca cgcgggagcg gacacgggaa caccacacug gaacaacaaa     1320

gaagcacucg uagaguucaa agacgcacac gccaaacgac aaaccguagu aguacuagga     1380

agccaagaag gcgccgugca cacagcacua gcaggggccc uagaagccga aauggacggc     1440

gccaaaggcc gacuaagcag uggacaccua aaaugccgcc uaaaaaugga caaacuacgc     1500

cuaaaaggag ucagcuauag ccuaugcaca gccgcauuca cguucacaaa aauucccgcg     1560

gaaacgcuac acggaacugu aaccgucgaa guacaauacg ccggcaccga cggaccgugc     1620

aaggucccug cacaaauggc uguagacaug caaacacuca caccggucgg ccgucuaaua     1680

acagccaacc ccguaaucac cgaauccacc gaaaacucaa aaaugaugcu ggaacucgac     1740

ccacccuucg gagacaguua cauagucauc ggagucggag agaaaaagau cacucaccac     1800

uggcacagau caggcucaac gaucggaaaa gccuucgaag caacaguccg cggggcaaaa     1860

agaauggccg uccuaggaga cacagccugg gauuucggua gcguaggagg agcccucaac     1920

ucccuaggaa agggcauaca ccaaauauuc ggagccgccu ucaaaagccu cuucggaggc     1980

augucguggu ucucgcaaau acuaaucggc acccuccuga uguggcuagg gcuaaacacc     2040

aaaaacggau cgaucucucu caugugccua gcccucggug gcgugcuaau auuccucucc     2100

accgccguca gcgcggaugu aggaugcagc guagacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4991
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4991
gggagaaagc uuaccaugcg cggacucagc ucccgcaaac gacgcucaca cgacguacua       60

accgugcaau uccucauccu ugggaugcua cucaugacag gcgcggugac ccgacgcggc      120

agcgcauacu acauguaccu cgaccgaaac gacgcgggcg aagcaaucag cuuccccaca      180

acacucggca ugaacaaaug cuacauacaa auaauggacc ucggacacau gugcgacgcc      240

accaugucgu acgagugccc aaugcuagac gaaggcguag aacccgacga cgucgacugc      300

uggugcaaua ccaccucaac auggguaguc uacggcacau gccaccacaa gaaaggagaa      360

gcccgccgcu cuagacgcgc cgucacucuc cccagucacu caacccgaaa acuccaaacg      420

cgcucccaaa cauggcucga gucccgcgaa uacacaaaac accucauccg aguagaaaac      480

uggaucuucc gaaaccccgg auucgcacuc gccgcagcag ccauagcaug gcuccuggga      540

ucaagcacau cccagaaagu aaucuaccuc gucaugaucc uacuaaucgc ucccgcguac      600

agcauacgau gcauaggcgu cagcaacaga gacuucguag aaggaauguc gggcggcaca      660

uggguagaca ucgugcucga gcacggcgga ugcguaaccg uaauggccca agacaaaccc      720

acaguagaca ucgagcucgu aacuaccaca gucaguaaca uggccgaagu ccgcagcuac      780

uguuacgagg ccucaauuuc agacauggcc uccgacaguc gcugcccaac acaaggcgaa      840

gcguaccugg acaaacaauc cgacacccaa uacguaugca aacgcacccu cguugacagg      900

ggguggggaa auggaugcgg auuauucggu aaaggguccc uaguaaccug cgccaaauuc      960

gcaugcucaa agaaaaugac cggcaaauca auacaaccag agaaccuaga auaccgcaua     1020

augcucagcg uccacgggag ccaacauucc ggaaugaucg uaaacgacac cggacacgaa     1080

accgaugaga aucgcgcaaa aguagaaauc acaccaaacu ccccacgggc ugaggcgaca     1140

cuaggcggcu ucggcagccu aggccuagac ugcgaacccc gcaccgggcu cgacuucucg     1200

gaccuguacu accucacgau gaacaacaaa cacuggcucg uacacaaaga augguuccac     1260

gauauccccc ucccauggca cgccggcgcc gacaccggaa caccccacug gaacaacaaa     1320

gaagcccucg uagaguucaa agacgcccac gccaaaagac aaacaguggu uguacuaggg     1380

ucacaggaag gcgccguaca cacagcacuc gccggagccc ucgaagccga aauggacggg     1440

gcaaaaggua gacuaaguag cggccaccuc aagugccgac ucaaaaugga caagcugcga     1500

cugaaagggg ucaguuacuc ccuaugcacc gcugcauuca ccuucaccaa gaucccggcc     1560

gaaacccuac acggaacagu caccgucgaa gugcaguacg ccggcacaga cggccccugc     1620

aaaguacccg cgcaaauggc cgucgacaug caaacacuca cgcccgucgg acggcucauc     1680

acagccaacc ccguaaucac ggaaagcaca gagaacagca aaaugaugcu agaacuagac     1740

ccacccuucg gagacuccua cauaguaauc ggcguaggcg agaagaaaau uacacaccac     1800

uggcacaggu ccggcucaac gauugguaaa gccuucgaag caacaguccg cggugccaag     1860

cgcauggcag uccuaggcga cacagcaugg gacuucggcu cagucggcgg cgcacucaac     1920

agucugggaa agggcauaca ucagauauuc ggcgcagccu ucaagucgcu auucggaggg     1980

augagcuggu ucagccagau ccucaucgga acacugcuca uguggcuagg acuaaacacc     2040

aaaaacggua gcaucagccu caugugccua gcacuaggcg gaguccuaau auuccuuagc     2100

acggcaguca gcgccgacgu gggcugcagu gucgacuucu cuaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  4992
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4992
gggagaaagc uuaccaugcg caucaucaac gcgcgcaaag aaaagaaacg acgcggagcc       60

gacaccucgg ucggaauagu cggccuccua cugacaaccg ccauggcagc cgaggucacc      120

cgacgaggcu ccgccuacua cauguaccuc gaccgcaacg acgccggaga ggccauuucc      180

uuccccacca cccucggcau gaacaaaugc uacauccaaa uuauggaccu aggacacaug      240

ugcgacgcaa caaugagcua cgaaugccca augcuagacg aagguguaga acccgacgac      300

guagacugcu ggugcaacac aacauccaca ugggucgugu acgguacaug ccaucacaag      360

aaaggcgaag cacgccgcag ccgacgcgcc guaacccucc caucccacag cacaaggaaa      420

cuucaaacac gcagccagac guggcucgaa agcagagaau acacaaaaca ccucauacga      480

guagaaaacu ggauauuucg caacccgggc uuugcccucg ccgccgcggc cauagcaugg      540

cuauuaggca gcaguacguc ccaaaaagua auuuaccuag ucaugauacu acuaauagca      600

ccagccuacu ccauaaggug caucggagug ucaaaccgcg acuucgucga aggaaugagc      660

ggaggaacau gggucgacau cguccucgaa cacggcgguu gcgucacagu cauggcccaa      720

gacaaaccca caguagacau cgaacucguc acgacaacag uauccaauau ggccgaagua      780

cgauccuacu gcuacgaggc aagcauauca gacauggccu ccgacucaag augcccaacc      840

caaggagaag ccuaccucga caaacagucc gacacucaau acgugugcaa acggacacuu      900

gucgaccgcg gguggggcaa cggauguggc cuauucggca aaggcagucu gguaaccugc      960

gcaaaauuug caugcuccaa gaaaaugaca gggaaaucca uacaacccga aaaccucgaa     1020

uacagaauca ugcucucagu ccacggguca cagcacagcg gcaugauagu aaacgacacc     1080

ggccacgaga cagacgaaaa ccgcgccaaa guagaaauaa ccccaaacag cccccgggcc     1140

gaggccaccc ugggcggguu cggcagccuc ggucucgacu gcgaaccccg aacaggucua     1200

gauuucucag accuauacua ccuaaccaug aacaacaaac auuggcucgu acauaaagag     1260

ugguuccacg acaucccacu cccauggcac gcaggcgcgg acaccgguac cccacacugg     1320

aacaacaaag aagcgcuagu cgaguucaaa gacgcacacg caaaaagaca aacagucgua     1380

guccuaggcu cccaggaagg agccguccac acggcacucg ccggagcacu cgaggcagag     1440

auggacggcg ccaaaggccg ccucagcuca ggacaccuaa aaugccgccu caaaauggac     1500

aaacucagac ucaaaggggu aucauacagc cuaugcacag ccgcauuuac guucaccaaa     1560

auaccagccg agacacucca cggcaccgua acagucgagg uccaauacgc aggaacagac     1620

ggccccugca aagucccagc ccaaauggcc gucgacaugc aaacacucac acccgugggc     1680

cgucuaauaa ccgcaaaccc cguuauaaca gaauccacag aaaacuccaa aaugaugcug     1740

gaacucgauc ccccauucgg ggacagcuac aucguaaucg gcguaggcga aaagaaaauc     1800

acccaccacu ggcaccgcuc cggaagcaca auaggcaaag cauucgaagc aaccguccgc     1860

ggagcgaaaa gaauggcugu acucggagac accgcguggg acuucggcag cgugggcggc     1920

gcacucaacu cccuaggaaa aggcauccac cagauauucg gcgcagccuu caaaucccuu     1980

uucggcggca uguccugguu cucccaaauc cuuauaggaa cccuucuaau guggcucggc     2040

cucaacacca aaaacggcuc cauaucccuc augugccucg cccucggcgg cguacuaauc     2100

uuccuaagca cagccgucag cgccgacguc ggaugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  4993
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4993
gggagaaagc uuaccaugau caucaacgcc cgaaaagaaa agaaacgacg aggcgccgac       60

acaagcguag gcaucguagg acuccugcuc acuacagcca uggcagcaga agugacccgc      120

cgcggauccg ccuacuacau guaccucgac cgcaacgacg ccggagaggc aauaucauuc      180

cccaccacgc uaggaaugaa caaaugcuac auacaaauaa uggaucucgg acacaugugc      240

gacgccacca ugagcuacga auguccaaug cuagacgagg gaguugaacc cgaugacgua      300

gacugcuggu gcaacaccac aucgaccugg gucgucuacg guaccugcca ccacaagaaa      360

ggcgaagccc gacgcucacg ccgagccguc acucuaccca gccacuccac aagaaaacuc      420

caaacacgca gccaaacaug gcuagaaucc cgcgaauaca caaaacaccu aauacgagua      480

gagaacugga ucuucagaaa ccccggcuuc gcgcucgcag cggccgccau cgcauggcuc      540

uuaggcucca gcaccagcca gaaaguuauc uaccucguaa ugauccuccu caucgcccca      600

gccuacucca uccgaugcau uggaguaagc aacagggacu ucguagaagg aaugagcggc      660

ggaacauggg ucgacauagu acucgaacac ggaggcugcg uaaccguaau ggcacaagac      720

aaacccaccg uggacauaga acucgucaca accaccguuu ccaacauggc cgaagugaga      780

agcuacuguu acgaagccag caucucagac auggcuuccg auagcagaug ccccacccaa      840

ggagaggcau accucgauaa acaaucagac acccaauacg ucugcaaaag aacacucgua      900

gaccgcgguu ggggcaacgg cugcggacuc uuuggcaaag gaucccuagu uaccugcgca      960

aaauucgccu gcagcaaaaa gaugacgggc aagucaauac agcccgaaaa ucuggaauac     1020

cggaucaugc uaucggucca cggcagccaa cacagcggca ugaucgugaa cgacaccggc     1080

cacgaaaccg acgaaaacag agcaaaaguc gagauaaccc caaacucacc ccgagcagaa     1140

gccacccuag gcggauucgg cagccugggc cuagacugcg aacccagaac cggccuagac     1200

uucuccgacc uguacuaccu cacaaugaac aacaaacauu gguuaguaca caaagaaugg     1260

uuccacgaca uaccucuacc auggcacgcc ggcgcagaca ccgggacccc gcacuggaac     1320

aacaaggaag cacugguaga guucaaggac gcacacgcaa aacgccaaac ggucgucgug     1380

cucggcagcc aagaaggagc cguccacacc gcgcuagcag gagcucucga ggcggaaaug     1440

gacggcgcca aaggcaggcu cuccucaggg caccucaaau gccgccucaa aauggacaaa     1500

cuccgacuca aagguguguc cuacucucuc ugcacagcag ccuucacauu caccaagauc     1560

ccugccgaaa cacuccacgg gacagucacg guagaagucc aauacgccgg uacagacgga     1620

ccgugcaaag ucccagccca aauggccgua gacaugcaga cacuuacccc agucggccgc     1680

cucaucaccg ccaaccccgu caucacagaa agcaccgaaa acucaaaaau gaugcuggaa     1740

cucgacccac ccuucgguga cagcuacauc guaaucggag uaggagagaa gaaaauaaca     1800

caccacuggc aucgcagcgg cuccaccauc ggcaaagcau ucgaggcuac aguaagagga     1860

gccaaacgaa uggccguccu aggagacacc gcaugggacu ucgggagcgu cggcggcgca     1920

cuaaacagcc ucggaaaagg gauacaucaa aucuucggcg ccgccuuuaa aucauuauuc     1980

ggcggcauga gcugguucag ccaaauauua aucggaacgc uacucaugug gcuagggcuc     2040

aacaccaaaa acggauccau cucacuaaug ugccuggcuc ucggaggcgu ccucaucuuc     2100

cuuucaacag caguaagcgc cgacguaggc ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  4994
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4994
gggagaaagc uuaccaugau caacgcccgc aaagagaaga aacgcagagg cgccgacacg       60

agcguaggaa ucgucggccu acuacucacu acagccaugg cggccgaggu cacacgcaga      120

gggucagccu acuacaugua ucucgacagg aacgacgccg gagaagcgau cucauucccc      180

accacccucg gaaugaacaa augcuacauc caaauaaugg accucgggca caugugcgac      240

gcuacaaugu ccuaugagug cccuaugcua gacgaaggcg uggaaccuga cgacguagau      300

ugcuggugca acacaacuuc aaccugggua guauacggca caugccacca caagaaagga      360

gaggcacgca ggagccggcg cgccgucacg cucccaucac acuccacgcg aaaacuucaa      420

acccggagcc aaacuuggcu agaaagcaga gaauacacca aacaccucau aagaguagaa      480

aacuggaucu uccgaaaccc cggauucgcg cucgcagccg cagccaucgc cuggcuacuc      540

ggcucgagca caagccaaaa agugaucuac cuaguaauga uacuccuaau cgcccccgcc      600

uacucaaucc gaugcaucgg aguaucaaac cgagacuucg uagaaggaau gucaggagga      660

acuugggucg auauaguacu agaacacgga ggcugcguca cggucauggc ccaagacaaa      720

ccaacuguug acaucgaauu aguaaccacg accgucagca auauggcaga ggugcgaucc      780

uacugcuacg aagccuccau cucggacaug gcaagcgacu cacgaugccc aacccaggga      840

gaggcguacc ucgacaaaca aagcgacaca caguacguau gcaaaaggac gcuagucgac      900

cgcggauggg ggaacggaug cgggcucuuu ggaaaaggcu cgcucguaac augcgccaaa      960

uucgcgugca guaaaaagau gacaggcaag agcauacaac cagaaaaccu agaauaccgg     1020

aucaugcucu ccguccacgg cucccaacac agcggaauga uagucaacga caccggacac     1080

gaaaccgacg agaacagagc aaaagucgaa auaacaccca acagcccccg cgccgaagca     1140

acccuaggag gguucgggag ccucggccug gacugcgaac cacgcaccgg acuagacuuc     1200

agcgaccuau acuaccuaac aaugaacaau aaacacuggc uaguccacaa agagugguuu     1260

cacgacauac cccugccaug gcacgcagga gcugacaccg gaaccccaca cuggaacaac     1320

aaagaagccc uggucgaguu caaggacgcc cacgccaaaa gacaaacagu agucguacuc     1380

ggcucacaag aaggcgcggu acacacagca cucgccggcg cgcucgaagc cgagauggac     1440

ggcgccaagg gcagauuaag cagcggccac cucaagugca ggcuaaaaau ggacaaacua     1500

cgacugaagg gcguaucaua cucacucugc acagcugcau ucacauucac caagauacca     1560

gcggaaacac uacacgggac aguaaccgua gaaguacaau acgccggcac cgacggcccc     1620

uguaaggucc cggcccaaau ggcagucgac augcagaccc uaaccccugu cggaagacug     1680

aucaccgcca acccaguaau cacagaauca acagagaacu caaaaaugau gcuggaacug     1740

gacccgcccu ucggagauag cuacaucgug aucggcgucg gugaaaagaa aaucacgcac     1800

cacuggcaca gaagcgggag caccauaggu aaagccuucg aggcgacagu uaggggcgcc     1860

aaacgaaugg caguccucgg cgacacagca ugggacuucg gcagcguggg cggagcacuc     1920

aacucacuag ggaaaggaau ccaccaaaua uucggcgcag cauuuaaaag ccuauucgga     1980

ggaaugagcu gguucucaca gauccuaauu ggcacacuac uaauguggcu agggcucaac     2040

accaaaaacg gaagcaucag ccuuaugugc cucgcacucg guggaguccu aauuuuucug     2100

agcaccgccg ucagugcaga cgugggaugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  4995
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4995
gggagaaagc uuaccaugaa cgcacgcaaa gaaaagaagc gccgcggagc cgacacaucc       60

guaggaaucg uaggacuacu ccucacaacg gccauggcag ccgaaguaac cagacgcggc      120

uccgccuacu acauguaccu cgaccgaaac gacgcaggag aggcuaucuc auuccccaca      180

acccucggaa ugaacaaaug cuauauacag auaauggacc ugggccacau gugcgacgca      240

acaaugagcu acgaaugccc aauguuagau gaaggcguag aacccgacga cgucgacugc      300

ugguguaaca ccacaucaac cuggguagua uacggcacau gccaccacaa gaaaggcgaa      360

gcgcgcagaa gccgucgagc agucacacua ccaucacacu caacacgaaa acuucagacc      420

cgaucacaaa ccuggcuaga aucacgagaa uauacgaaac accucauccg aguagaaaac      480

uggaucuuca gaaaccccgg cuucgcccuu gcagcggcgg ccaucgccug gcuccuaggg      540

aguucaacca gccagaaggu aaucuaccuc gucaugaucc uccucaucgc accagccuac      600

agcauccgau gcaucggcgu cagcaacaga gacuucguag aaggaaugag cggaggcaca      660

uggguagaca uaguacucga acacggcggg ugcguaaccg ugauggcaca agauaaaccc      720

acagucgaca ucgaacuugu gaccaccacc guaagcaaca uggcagaagu aagguccuau      780

ugcuacgaag caucaauuuc ugacauggca uccgacagcc gaugcccaac gcagggagaa      840

gccuaccuag acaaacaaag cgacacccaa uacguaugca aacgaacgcu gguagaccgc      900

ggcuggggca acgguugcgg acuauucggg aaaggaagcc uagucacaug cgccaaauuc      960

gccugcagca aaaagaugac aggcaaauca auccaacccg aaaacuugga auacagaauc     1020

augcuauccg uccacggaag ccaacacucc ggcaugauag ucaacgacac gggacacgaa     1080

acagacgaaa accgugcaaa aguagaaauc accccgaaua gcccaagagc ggaagccaca     1140

cuagggggcu uuggcagccu cggccuagau ugcgaaccac gaacagggcu agauuucucg     1200

gaccuauacu auuuaaccau gaacaacaaa cacuggcuag uucacaaaga augguuccac     1260

gacauaccgc ucccguggca cgccggcgca gauacgggca ccccacacug gaacaacaag     1320

gaagcgcugg uagaguucaa agaugcucac gccaaacgcc aaaccgucgu aguacuaggc     1380

ucacaggaag gugcaguaca caccgcacua gccggagcac ucgaagccga aauggacgga     1440

gcaaaaggac gacucagcag cggacaucua aaaugucgac ucaaaaugga caaacucagg     1500

cucaagggag ucagcuacag ccuaugcacg gccgcauuca cauucaccaa aauaccggca     1560

gaaacacucc acgguaccgu aacaguagag guccaauacg ccggcaccga cggccccugc     1620

aaaguaccag cacaaauggc aguagacaug caaacccuca cccccguagg acgacuaaua     1680

acggccaacc caguaauaac ugaaagcacc gaaaacagua agaugaugcu agaacuggac     1740

ccaccauucg gugacucaua caucgucauc ggcguaggcg aaaagaaaau aacccaccac     1800

uggcaccgaa gcgggucaac cauaggaaaa gccuucgaag cgacagugcg cggagccaaa     1860

cgcauggccg uacugggcga cacagcaugg gacuucggau caguaggcgg cgcccucaac     1920

agccucggca aagggaucca ccaaaucuuc ggagccgcgu uuaaaucgcu auucggcggc     1980

auguccuggu ucucccaaau ccucaucggc acccuacuca uguggcuugg ccuaaacacc     2040

aaaaauggcu caaucagccu caugugccua gcacucggag gcguccugau cuuccuguca     2100

acagcggucu cggcagacgu cggcuguuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  4996
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4996
gggagaaagc uuaccauggc acgcaaggaa aagaagcgcc gcggagcaga caccucaguc       60

ggaauagucg gccugcuacu caccaccgcc auggcggcgg aaguaacccg ccggggguca      120

gccuauuaca uguaccucga ccgcaacgac gcaggcgaag ccaucucauu ccccaccacg      180

cuaggcauga acaaaugcua cauccaaaua auggaccucg gccacaugug cgacgccacc      240

augagcuacg aaugcccaau gcuagacgaa ggcguagaac ccgacgacgu cgauugcugg      300

ugcaacacaa caucaacaug gguugucuac ggaaccugcc accauaagaa aggcgaagca      360

cgacgcagcc gcagagcagu uacacuaccc ucccacucga caagaaaauu acaaacacga      420

ucacaaaccu ggcucgaauc acgagaauac accaaacacc uaauccgcgu cgaaaacugg      480

auauuccgaa aucccggauu cgcucuagca gcagccgcga ucgccuggcu gcucggcucc      540

uccacauccc aaaaagucau uuaccucguc augauccuau uaaucgcacc ggccuacuca      600

auacgcugca uaggaguauc caacagagac uucgucgaag ggaugucagg cggcaccugg      660

guagacaucg uacuagaaca cggcggcugu gucaccguaa uggcccaaga caaaccaacc      720

gucgacaucg aacucgucac gaccaccgua agcaacaugg ccgaaguacg cuccuauugc      780

uacgaagcaa gcauaucaga uauggcaagc gacucccgcu gcccaacaca gggcgaagca      840

uaccuggaca aacaaaguga cacccaauac gucuguaagc ggacccucgu cgaccgcgga      900

uggggcaacg gcugcggccu cuucggaaaa ggaucccuag uaacaugcgc caaauucgca      960

ugcuccaaga aaaugaccgg caaguccaua caaccagaaa accuagaaua ccggaucaug     1020

cuaaguguac acggcagcca acauuccgga augauaguca acgacacagg gcacgaaacc     1080

gacgaaaacc gagcaaaagu agaaauaacc ccaaacuccc ccagagcaga agccacacua     1140

ggcggcuuug gcucucuagg ccucgacugc gaaccaagaa caggucuaga cuucucagac     1200

cuuuacuacc uaacaaugaa caacaaacac uggcucguac acaaagaaug guuccacgac     1260

aucccauugc cauggcacgc aggagcagac acaggaacac cccacuggaa caacaaagaa     1320

gcgcuagucg aguucaaaga cgcacacgcc aagagacaaa ccguaguagu ccucggcuca     1380

caagaaggug ccguccacac cgcacuagca ggcgcacuag aagccgaaau ggacggagca     1440

aaaggacgac uauccucagg acaccuaaaa ugccgucuca aaauggauaa acuaagacug     1500

aaaggcguau cauacucacu cugcacagca gccuucacgu ucaccaaaau ccccgccgaa     1560

acacuacacg gcacagucac aguagaagua caauacgccg gcaccgacgg ucccugcaag     1620

guaccagcuc aaauggcggu cgacaugcaa acccuaaccc ccguagguag acucaucacu     1680

gcaaaccccg ugaucaccga aucaaccgaa aacucaaaaa ugaugcugga acuagaccca     1740

ccguucggcg acagcuacau agucaucgga guaggggaga aaaagauaac ccaccacugg     1800

cacagaagug gaucgaccau cggcaaagcc uucgaagcaa caguucgagg cgccaaaaga     1860

auggcagucc ucggagacac cgcgugggac uucggcuccg uaggcggagc ccucaacucc     1920

cucggcaagg gaauacauca gaucuucggc gccgccuuca agagccuuuu cggaggaaug     1980

agcugguucu cacaaauccu aauaggcaca cuacucaugu ggcucggccu caacaccaaa     2040

aacgggucca ucucccuuau gugccuggca cugggcggag uacucauuuu ccucagcacu     2100

gccgucagcg ccgacgucgg cuguugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  4997
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4997
gggagaaagc uuaccaugag aaaagaaaaa aaacgccgag gagcagacac aagcgucggc       60

aucguaggau uacuacugac aaccgcgaug gcagccgaag ucacaagacg aggcucagca      120

uacuacaugu accucgaccg caacgacgca ggugaagcaa uaagcuuccc gacgacucua      180

ggaaugaaca aaugcuauau ucaaauaaug gaccuaggac acaugugcga cgcaaccaug      240

agcuacgaau gccccaugcu agacgagggu gucgaacccg acgacguaga cugcuggugc      300

aacaccacaa gcaccugggu cgucuacggc acaugccacc acaaaaaagg agaggcacgc      360

agaagcagaa gagcagucac gcuccccucc cacagcacaa gaaaacuaca aaccagaucc      420

caaaccuggc uggagucgag agaauacaca aaacaccuca uaaggguaga aaacuggauc      480

uucagaaacc cgggauucgc ccuagcggca gccgccaucg cauggcuccu gggcucaagu      540

acuagccaga aagugauaua ccuaguaaug auacugcuaa uagcacccgc cuacucaauc      600

cgcugcaucg gcgucucaaa ccgagacuuc gucgaaggua ugagcggcgg uacaugggua      660

gacaucguac uagaacaugg cggaugcguc accgucaugg cacaggacaa acccacagug      720

gacaucgaac uggucaccac aaccgucucu aacauggcag agguacgcuc cuacugcuac      780

gaagccucaa ucucagacau ggcgagcgac ucccgcuguc ccacccaggg cgaagccuac      840

cuggacaaac aauccgauac acaauacgug ugcaaacgca cacucguuga ccgagggugg      900

gggaacgguu gcggccucuu cggcaaaggc agccugguga ccugcgcaaa auucgcgugc      960

ucgaaaaaaa ugacaggcaa gagcauacaa ccagaaaacc uagaguaccg gaucaugcua     1020

ucaguccacg gaucgcaaca cucaggaaug auagucaacg auacaggaca ugaaaccgac     1080

gaaaaccgag cgaaaguaga aaucacaccc aacagcccgc gcgccgaagc aacacuaggg     1140

ggcuucggaa gccucggacu agacugcgaa ccacgcaccg gccuagacuu cagcgaccuu     1200

uacuaccuaa ccaugaacaa caaacacugg cuaguccaca aagaaugguu ccaugacauc     1260

ccgcuaccgu ggcacgcugg cgcagacacg gggaccccac auuggaacaa uaaagaagca     1320

cuagucgaau ucaaagacgc gcacgcaaaa cggcaaacag ucgucguacu aggcagccaa     1380

gagggagcug uccauaccgc gcuagccgga gcccuagaag cagaaaugga cggagcaaaa     1440

ggccgccuua gcuccggcca ccucaaaugu cggcuuaaga uggacaaacu cagacucaag     1500

ggcgucagcu acagccucug caccgcagcc uucacauuca ccaaaauccc ggccgaaacc     1560

cuacacggaa caguaacagu agaggugcaa uacgcgggaa cagacggucc augcaaaguc     1620

ccagcccaaa uggcuguaga caugcaaacc cuuaccccgg uagggagacu aauaacagcc     1680

aaccccguaa uuacugaauc aacagaaaac agcaaaauga ugcucgaacu cgacccaccc     1740

uucggcgaua gcuacauagu aaucgggguc ggagaaaaaa aaauaacaca ccacuggcac     1800

agaucaggau ccacaauagg aaaagcauuc gaagccaccg uuaggggagc aaaacgaaug     1860

gcaguacucg gcgacaccgc cugggacuuc ggcagcguag gcggcgcccu aaacagccuc     1920

ggaaaaggaa uccaccaaau auucggugca gcauucaaaa gccucuucgg cggcaugagc     1980

ugguucagcc aaauacucau cggcacgcuc cuaauguggc uaggacuaaa uacaaaaaac     2040

ggcaguauca gccuaaugug ccucgcgcua gggggcgucc uaaucuuccu cucaacagca     2100

gucagcgccg acgucggaug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  4998
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4998
gggagaaagc uuaccaugaa ggaaaaaaaa cgacgcggag cagacacauc agucggaauc       60

guaggccucc uccugacaac agcaauggcu gccgaaguaa cgaggcgggg aagcgcguac      120

uacauguacc ucgacagaaa cgacgcaggc gaagccaucu ccuucccaac cacauuagga      180

augaauaaau gcuacaucca aauaauggac cucggacaca ugugcgacgc cacaauguca      240

uacgaaugcc cgaugcucga ugaaggcguc gaacccgaug acguagacug cuggugcaac      300

acaacgagca caugggucgu cuacggaaca ugccaccaca aaaaaggcga ggcccgacga      360

ucacgccgag cagugacacu ccccucccac agcacccgca aauuacaaac gagaagccaa      420

acauggcucg aaagccgcga auauaccaaa caccugauca gaguggaaaa cuggauauuc      480

aggaaccccg gcuucgcccu ggcagcagca gcaauagccu ggcuacuugg caguagcaca      540

ucccagaaag ucaucuaccu cgucaugaua cuccucaucg caccugcaua cagcauccga      600

ugcaucggcg uguccaacag agacuucgua gaaggcauga gcggcggaac augggucgac      660

auaguacucg aacacggcgg cugcguaacc gugauggcuc aagacaaacc cacagucgac      720

aucgagcuag ucaccacgac cguaagcaac auggcagaag uacgcagcua cugcuaugaa      780

gccucgauau ccgacauggc aucagacucc cggugcccca cccagggaga agcauaccuc      840

gacaaacaau ccgacaccca guacguuugu aaacgcacac uagucgacag aggcugggga      900

aacggcugcg gccuauuugg caaaggcagc cuggucacau gcgcaaaauu cgccugcagc      960

aaaaaaauga ccggaaaaag cauacaacca gaaaaccugg aauaccgcau aaugcugucc     1020

guccacggaa gccaacacuc aggcaugauc guaaacgaca ccggccacga gaccgacgaa     1080

aacagagcaa aagucgaaau cacaccgaac agcccacgag ccgaagcaac ccucggaggg     1140

uucggcucgu uaggacucga cugcgaacca agaaccggac ucgacuucuc agaccucuac     1200

uaccuaacaa ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauacca     1260

cucccauggc acgccggagc cgacaccggc accccacacu ggaacaacaa agaagcccuc     1320

gucgaauuca aagacgccca cgccaaacga caaaccguag uaguccucgg aagccaagaa     1380

ggcgcuguac acacggcccu cgcaggggcg cuggaagccg aaauggaugg cgcaaaagga     1440

agacuaagcu ccggccaccu aaaaugccgu cucaagaugg acaaacugcg ccucaaaggc     1500

gucucauaua gccuaugcac cgccgcuuuc acauucacaa aaauacccgc cgaaacacuc     1560

cacggcacag uaacagucga aguacaauau gccggcacag acggcccaug caaaguccca     1620

gcccaaaugg ccgucgacau gcaaacccuc acaccagucg gccgccuaau cacagcaaac     1680

cccguaauaa cagaaagcac agaaaacuca aaaaugaugc uagaacugga cccccccuuc     1740

ggcgauagcu acauagucau cggaguuggc gaaaaaaaaa uaacacacca cuggcaccgc     1800

uccggcucca cgaucggcaa agcauucgaa gcaacgguac gaggagcaaa aagaauggca     1860

guacuaggag auacugcaug ggacuucggc uccguggggg gggcacuaaa cucccuaggc     1920

aaaggcaucc accaaaucuu cggcgcagcc uucaaaucac ucuucggcgg gauguccugg     1980

uucagccaaa uccucaucgg cacccuacua auguggcuug ggcuaaauac gaagaacgga     2040

agcauauccc ucaugugccu agcacuaggc ggcguccuca ucuuccuuag caccgcagua     2100

uccgcagacg uaggcugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  4999
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  4999
gggagaaagc uuaccaugga aaagaaaaga cgaggagcag acaccagcgu cggcaucguc       60

ggucuacuac ucacaacagc cauggccgca gaagugacca gacggggauc ggcauacuac      120

auguaccucg accguaacga cgcaggagaa gcaaucucau ucccaaccac acucggcaug      180

aacaaaugcu acauccaaau aauggacuua ggacacaugu gcgacgccac gauguccuac      240

gaaugcccga ugcuagauga aggaguggaa cccgacgacg ucgacugcug gugcaacacc      300

acgagcacgu ggguaguaua cggaacaugu caccacaaaa agggugaagc cagacgcucc      360

cgacgggcgg ucacccuucc cucacacagc acaagaaaac ugcaaacucg cagccaaaca      420

uggcuggaau cacgcgaaua cacaaaacac cuuauacggg ucgaaaacug gaucuuccga      480

aacccaggau ucgcacuagc agcggccgcc auagccuggc uacuaggcag caguacuuca      540

caaaaaguaa uauaccuagu caugauacuc cugauagccc ccgcuuacuc cauccgaugc      600

aucggaguaa gcaauagaga uuucguugaa ggaaugucag gaggaacaug gguagacaua      660

guccucgagc acggcggcug cguaaccguc auggcacagg acaaaccaac cguggacaua      720

gaacugguca caacaacugu cagcaacaug gccgagguac gcaguuacug cuacgaggca      780

uccaucuccg acauggcauc ggacagccga ugcccgaccc aaggagaggc auaccucgac      840

aaacaaagcg acacgcaaua uguauguaaa cgaaccuuag uagaccgcgg auggggcaac      900

gguugcggac ucuucggaaa aggcucccug gucacaugcg caaaguucgc cugcuccaag      960

aaaaugacgg gaaaaagcau ccaaccggaa aaccuagagu accgaaucau gcugucuguc     1020

cacggauccc agcauuccgg caugaucguc aacgacacug gacacgaaac agacgaaaac     1080

cgagccaaag uggagauaac cccaaacagc ccaagagcag aggcaacccu aggaggauuc     1140

ggaucccuag gacuggacug cgaaccgcgc acuggacucg acuucucaga ccuauacuac     1200

cuuacaauga acaacaaaca cuggcucguc cacaaagagu gguuccacga uaucccccua     1260

cccuggcacg ccggcgcaga cacaggaaca ccacacugga acaacaaaga agcccuaguc     1320

gaguucaaag acgcgcacgc caaacggcaa acaguagucg uccucggaag ccaagaaggg     1380

gcuguacaua ccgcacucgc cggcgcccua gaagccgaaa uggacggcgc gaaaggacga     1440

cuaagcucag gacaccucaa augccggcuc aaaauggaca aacucagacu caaaggaguu     1500

uccuacuccc uaugcacagc agccuuuacc uucaccaaaa uacccgcgga aacccuccac     1560

ggaaccguca cagucgaagu ccaauaugcg ggaaccgacg ggcccugcaa agugcccgcc     1620

caaauggccg ucgauaugca aacccucacc cccguaggcc gacuaaucac agccaacccc     1680

guaauaaccg agucaacaga aaacuccaaa augaugcuag aacuagaucc cccauucgga     1740

gacagcuaca uaguaaucgg cguaggcgaa aagaaaauca cccaccacug gcaccgaucc     1800

ggaagcacaa ucggaaaagc cuucgaagca accguccgug gcgccaaaag aauggccgua     1860

cuuggagaca cagcauggga cuucgggagc gucggaggag cacugaacag ccucggaaaa     1920

ggaauccacc aaaucuucgg cgcagcguuc aagagccuau ucggcgggau gagcugguuc     1980

ucacaaauac uaaucggaac acugcucaug uggcuaggcc uaaacaccaa aaacggauca     2040

aucagccuca ugugucucgc gcugggagga guacugaucu uccuaucaac agccguaagu     2100

gccgacgucg gcugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  5000
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5000
gggagaaagc uuaccaugaa aaaaagacgg ggugccgaca ccucgguagg aauaguagga       60

cuccuacuca ccaccgccau ggcagcagaa guaacacgcc gaggcagcgc cuacuauaug      120

uaccucgaca gaaacgacgc cggcgaagcc aucuccuucc ccaccacacu cggcaugaac      180

aaauguuaca uccaaauaau ggaucucggc cacaugugcg acgcaaccau gucauacgag      240

ugcccaaugc ucgacgaagg agucgaaccc gaugacguag acugcuggug caauaccacc      300

uccacauggg ucguauacgg aacuugccac cauaaaaaag gagaagcaag acgaucacgg      360

agagccguca ccuuacccag ccauuccacc cggaaacuac aaaccagaag ccaaacgugg      420

cucgagagcc gggaauauac aaaacaccuc auaagagucg aaaauuggau auucagaaac      480

cccggauucg cccucgccgc cgcagccaua gccuggcuac ucggauccag cacaagccag      540

aaagucauau accucgucau gauacugcua auugccccag cuuauuccau ccggugcaua      600

ggcgucagua accgggacuu cguagaagga augucaggag ggaccugggu agacaucgug      660

cuagaacacg gaggcugcgu aaccgucaug gcacaagaca aaccaacagu cgacauagaa      720

cugguaacca cuacagucag caacauggcc gaaguacgau cauacugcua ugaagcauca      780

aucagcgaca uggcaagcga cagucgaugc cccacgcagg gcgaagccua ccuagacaaa      840

caaucagaca cacaauacgu gugcaaacgg acacuugucg acagagggug gggaaacggc      900

ugcggccuau ucgggaaagg cagccuagua accugcgcga aauucgcgug cucaaaaaaa      960

augaccggca aaagcaucca gccagaaaac cuagaauaca ggaucaugcu cagcguccac     1020

ggcagccagc acagcgguau gauagucaac gacacuggcc acgaaacaga cgagaaccgc     1080

gcaaaagugg aaaucacacc aaacucacca cgcgccgaag caacccucgg uggguucggc     1140

ucccuuggac uagacuguga accaagaacc ggccucgacu ucagcgaccu auacuaccuc     1200

acaaugaaca acaaacacug gcucguccac aaagaauggu uccacgacau accccuacca     1260

uggcacgcag gcgcagacac agggacucca cacuggaaca auaaggaagc gcuagucgaa     1320

uucaaagacg cccacgcaaa aagacaaacc guggucgucc ugggauccca agaaggagca     1380

gugcacaccg cccucgcagg cgcucucgaa gccgagaugg auggagcaaa aggaagacua     1440

agcagcgggc accucaaaug ccgacuaaaa auggacaaac uaagacuaaa aggcgucagc     1500

uacagcuuau guacggccgc cuucaccuuc acgaaaaucc cugccgaaac ccuacacggc     1560

accguaaccg ucgaagucca auacgccgga acagacgggc cguguaaagu ccccgcccag     1620

auggcaguag auaugcaaac acucaccccc guaggacgcc uaaucacggc aaaccccguc     1680

auaaccgaaa gcacagaaaa cucaaaaaug augcucgaac uagauccccc cuucggagac     1740

agcuacauag uaauaggagu aggagaaaaa aaaaucaccc accacuggca cagaucagga     1800

aguaccaucg gcaaggccuu cgaagccacc gucagaggag ccaaacgaau ggcgguccuc     1860

ggagacaccg ccugggacuu cggaagcguc ggcggcgcac ucaacucacu aggcaaaggc     1920

auacaccaaa ucuucggagc cgccuucaaa agccucuucg gcggaaugag cugguucucc     1980

caaauacuaa ucggcacccu acucaugugg cucggucuca acaccaaaaa cggcuccaua     2040

ucccucaugu gccuagcacu cggaggcguc cucaucuucc ugagcaccgc cgucagugcc     2100

gacguaggcu guugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  5001
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5001
gggagaaagc uuaccaugaa gaggagaggc gcagauacga guguagguau aguagggcuc       60

uugcucacga ccgcaauggc agccgaaguc acaagaaggg gcucggcaua cuacauguac      120

cuggacagga acgacgccgg cgaagcaaua ucguucccaa caacgcuagg gaugaauaaa      180

ugcuacauac agaucaugga ucuagggcac augugugacg ccacaauguc auacgaaugc      240

ccgaugcugg augaaggcgu agagcccgac gacguggacu gcuggugcaa cacgacaagc      300

acauggguag ucuacggcac augccaucac aagaaaggcg aagcgagaag aaguaggaga      360

gcaguaacac ugccaagcca cucaacccgc aagcuacaaa cccgaaguca gaccuggcua      420

gaaucaagag aguacacaaa acaccugaua agaguagaga acuggauauu ccgaaaccca      480

ggauucgccu uggcagccgc ggcaauagcc uggcuauuag gcucguccac cagccaaaag      540

gucaucuauc ucgugaugau acuacuaauc gcccccgcau acucaaucag gugcauagga      600

gucagcaacc gcgacuuugu agaggggaug ucgggcggga cauggguaga caucguacua      660

gaacacgggg gcugugucac agugauggcc caagacaaac ccacgguaga uaucgaacuc      720

gugaccacga caguuagcaa cauggcagaa guuagaucgu acugcuacga agccagcaua      780

agcgacaugg ccucagacuc gcgaugccca acgcaagggg aggcauaccu ggacaaacaa      840

ucagacaccc aauacgucug caaaagaacc cuagucgacc gaggcugggg caacgggugc      900

gggcuauucg gcaaaggcag ccugguaaca ugugcuaaau ucgcaugcag caaaaagaug      960

acaggcaaaa guauacagcc agagaaccug gaguacagga ucaugcuaag cguccacggc     1020

agccaacaca gcggcaugau cgucaacgac acaggccacg aaacugacga aaaccgagcc     1080

aaaguagaga uaacacccaa cagcccgcgc gcagaggcaa cacugggcgg guucgggagc     1140

cugggacuag acugcgaacc gagaacgggc cuggacuuca gcgaccucua cuacuuaacc     1200

augaacaaca aacacuggcu aguccauaaa gagugguucc acgacauacc acuaccaugg     1260

cacgcaggcg cagacaccgg gacaccacac uggaacaaca aagaagcccu ggucgaguuc     1320

aaggacgcac acgccaaaag acagacaguc gugguccugg gcucgcaaga aggagccguc     1380

cacaccgccc ucgcgggagc acuagaagcc gagauggacg gggccaaagg gcgacugagc     1440

ucgggacauc ucaaaugccg ccuaaagaug gauaaacucc gccucaaagg cguaucauac     1500

agcuuaugca ccgcggcauu cacauucaca aagauccccg ccgagaccuu gcacgggacc     1560

gugacaguag aagugcaaua cgccggaacg gauggaccau gcaaaguccc agcacaaaug     1620

gcagucgaca ugcaaacccu aaccccagua ggacgccuaa uaacggcaaa ccccgucauc     1680

acagaaucaa ccgaaaacuc caaaaugaug cuggaacuag acccaccguu uggcgauagc     1740

uacaucguaa ucggaguagg agaaaagaaa aucacacauc acuggcaccg aagcggcagc     1800

acaaucggca aagcauuuga agcaacagua cggggcgcaa aacgaauggc aguacuugga     1860

gacaccgcau gggauuucgg gagcguagga ggggcccuga acucgcuagg gaaaggaauc     1920

caccaaauau ucggggccgc guucaagagc cuuuucggag gaaugagcug guucagccaa     1980

auucucauag ggacgcuccu gauguggcuc gggcuaaaca ccaaaaacgg cucaaucagc     2040

uuaaugugcc uagcacuagg aggcguccua auauuccuaa gcacagccgu aagcgcagac     2100

guaggcugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  5002
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5002
gggagaaagc uuaccaugcg ccguggugca gacaccucag uaggaaucgu aggccuucuc       60

cuaaccacag cuauggccgc agaagucaca cgacggggau cggccuacua cauguaccuc      120

gaccgcaacg acgccggcga agcaaucagc uucccaacca cccugggcau gaacaagugc      180

uacauacaaa uaauggaccu cggccacaug ugcgacgcaa cgaugucaua ugaaugcccc      240

augcucgacg aaggaguaga acccgacgac guagacugcu ggugcaacac caccagcacc      300

ugggucgucu auggaaccug ccaccacaag aaaggcgagg cccgcagguc ccgacgcgcc      360

guaacgcuac ccagccacuc aacucgcaaa cuacaaacca gaagccaaac cuggcuagaa      420

ucccgagaau acacgaaaca ccucauaaga gucgaaaacu ggauauuccg caacccaggc      480

uucgcccugg cagcagcagc aaucgccugg cuccuaggca gcagcaccuc ccaaaaagua      540

auauaccuug uaaugauacu gcucauagca cccgcauaca gcauacgaug caucggaguc      600

agcaacagag acuucgucga aggcaugagc ggaggcacau ggguagacau cguccuagaa      660

cacggagggu gcgucacggu aauggcacag gacaaaccaa cagucgacau cgaacucguu      720

accacaaccg uaagcaacau ggcagagguc agauccuacu gcuacgaagc auccauaagc      780

gacauggcau ccgacagccg augcccaacc caaggcgagg cuuaccuaga caaacaaucc      840

gacacccagu acguaugcaa gcgaacucua gucgauaggg gauggggcaa cggaugcgga      900

cucuucggca aaggcuccuu agugacaugu gcaaaauucg ccuguagcaa gaaaaugacc      960

ggcaaaagca uccaaccaga aaaccuggag uaccguauaa ugcucagcgu acacggaagc     1020

caacacagcg gcaugauagu caacgacaca ggacacgaaa cagacgaaaa ccgugccaaa     1080

guagaaauca ccccaaacag cccacgagcu gaagccaccc uaggaggcuu cggaagccug     1140

ggacuagacu gcgaaccccg caccggccuc gacuucuccg acuuauacua ccugacaaug     1200

aacaacaaac acuggcuagu acacaaagag ugguuccaug acaucccacu acccuggcac     1260

gcaggagccg acaccggcac accgcacugg aauaauaaag aagcgcucgu agaguucaag     1320

gacgcccacg ccaagcgaca aaccguggua guccugggca gccaagaagg agcaguccac     1380

accgcccuag caggagcacu agaagccgaa auggacggag caaaagggcg acucagcuca     1440

ggacaucuca aaugccgccu gaaaauggac aaacuccggc ucaagggagu cagcuacagc     1500

cugugcaccg cagccuucac cuucaccaaa auacccgcag aaacccucca cggcaccgua     1560

acaguagaag uccaauacgc aggaaccgac ggacccugca aaguaccagc ccaaauggca     1620

guagacaugc aaacacucac acccgucggu agauuaauaa cagccaaccc agucauaacc     1680

gaaucgacug aaaacaguaa aaugaugcuc gagcuagauc ccccauucgg ggacagcuac     1740

auaguaaucg gagucggcga gaaaaagauc acacaucacu ggcaccgcag cggcagcacc     1800

aucggcaagg cuuucgaagc aacaguacgc ggggcaaaac ggauggccgu ccuaggcgac     1860

acagccuggg acuucggcuc cguaggcggg gcacucaaca gccuagggaa aggcauccac     1920

caaaucuucg gagccgccuu caaaucgcug uucggaggaa ugagcugguu uagucaaaua     1980

cuuauaggca cccuacucau guggcucgga cuaaauacca aaaacgguuc caucagccuc     2040

augugccuag cccuaggcgg gguacugauc uuccucucaa ccgccgucuc cgccgacgua     2100

ggcuguugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  5003
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5003
gggagaaagc uuaccaugag aggagcugac acgagcgucg gcauagucgg acuccuccua       60

accacagcca uggcagccga aguaacacga cguggauccg ccuacuacau guaccuggac      120

agaaacgaug caggcgaagc uaucagcuuc cccaccaccc ucggcaugaa caagugcuac      180

auacagauca uggaccuagg ccacaugugc gacgcgacga ugagcuacga augcccgaug      240

cucgacgaag gcguggaacc cgacgacguc gacugcuggu gcaacacaac cagcaccugg      300

guugucuacg gcacgugcca ccacaagaaa ggagaagcac gccgaucccg cagagccgua      360

acccuacccu cacauuccac aagaaaacua caaacccgaa gccaaacaug gcuagaaagc      420

cgcgaguaca caaaacaccu aauaagagua gaaaauugga uauuccgaaa ccccgguuuc      480

gcccucgcag ccgccgcgau cgccuggcua cuaggauccu ccacuagcca aaaaguaauc      540

uaccucguca ugauacuacu cauagcccca gccuacucca uccgcugcau cggaguguca      600

aacagggacu ucguagaagg caugagcgga ggcacguggg ucgacaucgu acuagaacac      660

ggcgggugcg ucacagugau ggcccaggac aaaccaaccg uagacaucga gcuagucacg      720

accacaguaa gcaacauggc ugaagucaga ucguacugcu acgaagccuc caucuccgac      780

auggcauccg acucccgcug cccaacccaa ggcgaagcau accucgacaa acaaucggac      840

acccaauaug uaugcaaacg cacacucgua gacagaggau ggggcaacgg cugcggacua      900

uucggaaaag ggagccuagu cacuugugcc aaauucgccu gcagcaagaa aaugaccgga      960

aaaaguaucc aacccgaaaa ccuagaauac cgcaucaugc ucuccgucca uggaucccaa     1020

cacagcggca ugaucguaaa cgacaccggc cacgaaacag acgaaaaccg cgccaaaguc     1080

gaaaucaccc ccaacucccc aagagcagag gccacccugg gaggauucgg aucacuaggc     1140

cucgauugcg aaccgcggac cggccuagac uucucagacc uguauuaccu uaccaugaac     1200

aacaagcacu ggcucguaca caaggaaugg uuccacgaua uaccacuacc cuggcacgcc     1260

ggcgccgaca caggcacacc ccacuggaac aacaaagagg ccuuagucga guucaaagac     1320

gcccacgcaa agcgucaaac cguagucgua cuuggaucgc aagaaggcgc cguccacacc     1380

gcccucgcag gggcccucga agcagaaaug gacggcgcga aagggcgccu auccagcgga     1440

caccucaaau gcagacuaaa aauggauaaa cuccgccuga aaggcgucuc auacucccuc     1500

ugcaccgccg ccuucaccuu cacaaaaauc cccgcagaaa cccuccacgg gacaguaacc     1560

gucgaagucc aguacgccgg aacagacgga cccugcaaag uaccugcgca aauggcagua     1620

gacaugcaaa cccuaacccc cgugggacga cuaauaacgg caaacccagu cauaaccgaa     1680

uccacagaaa acucaaaaau gaugcucgag cucgacccac ccuucggcga cagcuacauc     1740

gucaucggag ucggagagaa aaagauaaca caccacuggc acagauccgg cagcacgauc     1800

ggcaaagcau ucgaagccac cguacgugga gcuaaacgca uggcaguccu cggagauaca     1860

gccugggacu ucggcuccgu cggaggcgcc cucaacagcc uaggcaaagg aauacaccaa     1920

auauucggug cagcauucaa gucccucuuc ggcggcauga gcugguucuc acagauccuc     1980

auugggaccc uacucaugug gcuaggucua aacaccaaaa acggcuccau cucccucaug     2040

ugccuggcac uagguggcgu acucaucuuc cucucgaccg cgguuucagc cgacgucggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  5004
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5004
gggagaaagc uuaccauggg cgcggacaca agcgucggga ucguaggccu ucugcucacg       60

accgccaugg cagccgaagu cacgcgacgg ggaucggccu acuacaugua ccuugacaga      120

aacgacgccg gugaagcaau auccuucccu accacccucg gaaugaacaa auguuauaua      180

cagaucaugg accucggcca caugugcgac gcaaccaugu cauacgaaug ccccaugcua      240

gacgaaggag uggagccaga cgacguggac uguuggugca acacaacgag cacaugggua      300

guuuacggaa caugccauca caagaaagga gaagcuagaa gauccagacg cgcagucaca      360

cuaccauccc acagcacgcg caaacuccag acacgcagcc aaacauggcu ggagucaagg      420

gaauacacaa aacaccucau acgagucgag aacuggaucu uccgcaaccc cggauucgca      480

cucgcagccg cagcaauagc auggcuucuc gggagcucca ccagccaaaa aguaaucuac      540

cucguaauga uacuccuaau cgcaccagcg uacuccauaa gaugcauagg cguaagcaac      600

cgagacuucg ucgaaggaau gagcggugga acguggguug acaucguacu ggaacacggg      660

gguuguguca cggucauggc acaagacaaa ccaacggugg acaucgagcu aguaacaacg      720

accgucagca acauggcaga aguccgaucc uacugcuacg aagcaucaau cagcgacaug      780

gccucagacu cccgaugccc cacccaagga gaagccuacc uagacaagca auccgacacu      840

caauacgucu gcaaaagaac gcuagucgac cguggauggg gaaacggcug cgggcuauuu      900

ggaaaaggau cucuagucac uugcgcaaaa uucgcgugua gcaagaaaau gacaggaaaa      960

agcauacaac cggaaaauuu agaauacagg auaaugcucu ccguccacgg cucccaacau     1020

agcggcauga ucgucaacga cacaggucac gaaacagacg aaaacagggc caaaguagaa     1080

aucaccccca acucaccgcg cgcagaagca acacugggcg gcuucggcuc acuaggccuc     1140

gacugcgaac cacgcacugg cuuagacuuc agcgaccucu acuaccugac aaugaacaau     1200

aaacacuggc uaguccacaa agaaugguuc cacgacauac cgcuaccaug gcaugcagga     1260

gcggacacag gcacaccaca cuggaacaac aaagaagccc ucgucgaguu caaagacgcc     1320

cacgcaaaaa gacaaacggu cguaguccua ggcucccaag agggggcagu acacacagca     1380

cucgcaggag cacucgaagc cgagauggac ggagcgaaag gccgacuauc aucgggacac     1440

cucaaaugca ggcucaaaau ggacaaacug cggcuaaaag gagucuccua cucacucugc     1500

acagcggccu ucacuuucac caaaauccca gccgaaaccc uacacggcac cgucaccgug     1560

gaaguacaau acgccggcac cgacggcccg ugcaagguac cagcccaaau ggccguagac     1620

augcagaccu ugaccccagu aggaagacua aucacagcca acccggucau aacagaaagc     1680

accgagaacu ccaaaaugau gcuggaacug gacccacccu ucggagacag cuauaucguc     1740

auaggaguug gagagaaaaa gaucacccac cacuggcacc gaagcggauc aaccaucggc     1800

aaagccuucg aagccacagu ccgaggcgca aaaagaaugg ccguccuagg cgacacggcc     1860

ugggacuucg ggaguguagg uggagcacuc aacucacucg gaaaaggcau ccaccaaaua     1920

uucggggccg ccuucaagag ccuauucgga ggcaugagcu gguucucgca aauacuaauc     1980

ggcacacugc ucaugugguu aggccuuaac acuaaaaacg gcaguaucag ccuaaugugc     2040

cucgcacucg gaggcgugcu cauauuccug agcacagcag ugagcgcgga cgugggaugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  5005
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5005
gggagaaagc uuaccauggc agacaccagc gucggcauag uagggcuacu acucacaaca       60

gcaauggcag ccgagguaac acggcgcggc ucagccuauu acauguaccu cgacagaaac      120

gacgcaggag aagcaaucag cuuccccacc acgcuaggca ugaacaaaug cuacauacaa      180

auaauggacc uaggccacau gugcgacgcu acaaugaguu acgaaugccc aaugcuggac      240

gaaggcguag aaccagacga cgucgacugc uggugcaaca ccacuagcac cugggucguc      300

uacggaaccu gccaccauaa gaaaggggaa gcgcgccgca gccggcgagc gguaacacuc      360

cccagccaca gcacgcgcaa acuccagacc cgcagccaga cauggcuaga auccagagaa      420

uacaccaaac accuaauaag aguagagaac uggaucuucc gcaacccagg auucgcccuc      480

gccgccgcag ccauagccug guuauuaggc uccuccacaa gccagaaagu aaucuaccuc      540

guaaugauac uccuaaucgc acccgcauau uccauaagau gcauaggcgu aagcaaccgc      600

gacuucguug aagggauguc gggcggaacc ugggucgaca ucgugcucga acacggagga      660

ugcgucacgg ucauggccca agacaaaccc accgucgaca ucgaguuagu cacuacaaca      720

guguccaaca uggccgaggu acgcuccuac ugcuacgaag caagcauauc cgacauggcc      780

uccgacaguc gcugcccaac acagggagaa gcauaccucg acaaacagag cgacacacaa      840

uacguaugca aacgaacccu aguagaccga ggauggggca acggaugcgg acuauucggc      900

aaaggcuccc uagugaccug cgcaaaauuc gcaugcucaa agaaaaugac gggaaaaagc      960

auacaaccag aaaaccucga guaccgaaua augcuaucgg uccacggcag ccaacacagc     1020

ggaaugauag uaaacgauac cggacacgaa acagacgaaa acagagccaa aguagagaua     1080

acucccaaca gcccacgagc ugaagcgacu cugggaggau ucggcagccu gggccucgac     1140

ugugaaccgc gcaccggccu agacuucuca gaucuuuauu accucacuau gaauaacaaa     1200

cauuggcucg uacacaaaga augguuccau gacauaccac uaccauggca cgccggagca     1260

gacaccggca ccccacacug gaacaacaaa gaggcccucg uagaguucaa agacgcccac     1320

gcgaaacgac aaacagucgu gguucuaggc ucacaagaag gagcggucca caccgcgcua     1380

gcuggcgcac ucgaagcaga aauggacgga gcaaaaggac ggcucagcuc cggccaccuc     1440

aaaugccgac uaaaaaugga caagcuccgg cugaaaggag ucagcuacuc ccucuguacc     1500

gccgccuuca ccuucacaaa aaucccagca gaaacacuac acggcacagu aacaguagag     1560

guccaauacg cggggaccga cgguccaugc aagguaccag cccaaauggc aguagacaug     1620

cagacccuaa cgcccguagg acgccugaua accgcaaacc caguaauaac cgaaagcacc     1680

gaaaacagca aaaugaugcu ggaauuagac ccgccauucg gcgacagcua uaucgucauu     1740

ggcgucggcg aaaagaaaau cacccaccac uggcaccgca guggauccac aauaggaaaa     1800

gcauucgagg caaccguacg aggcgcaaaa cgaauggccg uccucggaga uacagcgugg     1860

gacuucggcu ccgucggcgg agcacucaac agccuaggga aagguauaca ccaaauauuc     1920

ggcgcagcuu ucaaaagccu auucggagga augagcuggu ucagccaaau acuaaucggc     1980

acacuccuca uguggcucgg acucaacacc aaaaacggga gcauaagccu caugugccug     2040

gcgcucggag gcguacucau cuuccucuca acggcaguaa gcgcagacgu gggaugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  5006
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5006
gggagaaagc uuaccaugga caccuccguc ggcauagugg gacuccuccu uacuaccgcc       60

auggcagcgg aaguaacccg acgcggcuca gcauacuaca uguaccugga ccgcaacgac      120

gcaggcgagg ccaucagcuu ucccaccacc cugggaauga acaaguguua cauacaaauc      180

auggaccucg gccacaugug cgacgcaacc augagcuacg aaugcccaau gcucgacgaa      240

ggaguagaac ccgacgacgu agacugcugg ugcaacacaa ccucgacaug ggucguauac      300

ggaaccugcc accacaagaa aggcgaggcc cgccguucac gccgagccgu cacacuacca      360

ucccacucca caagaaaauu acaaacgcgc ucacaaacau ggcucgaguc cagagaauac      420

accaaacacc uaauccgagu agaaaacugg aucuuccgaa accccggcuu cgcccucgcc      480

gcagccgcaa uagcguggcu ccucgguucc agcaccuccc aaaaagucau cuaccuagua      540

augauacuac uaaucgcucc agcuuacucc auaagaugca uaggcguauc caaccgggac      600

uucgucgaag gcaugagugg cggcacaugg gucgacaucg uccuagagca cgggggcugc      660

guaacaguca uggcccaaga caaaccaacg gucgacauag aacucgucac caccacaguc      720

ucaaacaugg cagaaguccg cuccuacugc uacgaagcga gcaucaguga cauggcauca      780

gacagccgcu gcccaaccca aggagaagcc uaccuagaca aacaauccga uacucaauac      840

gucugcaaaa gaacauuagu ggacagaggc uggggaaacg gcugcggccu cuuuggaaaa      900

ggcagccuag uaacaugcgc caaauucgcu ugcucuaaga aaaugaccgg caaaucaauc      960

caacccgaaa accuggagua ucgcaucaug cuaagcguac acggcagcca acacagcgga     1020

augauaguua augacaccgg ccacgaaaca gacgaaaacc gcgcaaaagu cgaaaucacc     1080

cccaacucac cccgcgcaga agccacccua ggcgguuucg gcucacucgg cuuagacugc     1140

gaaccacgua caggacuaga cuucucagac cuguacuacc ucaccaugaa uaacaagcac     1200

uggcucgucc acaaagaaug guuccacgac auaccccugc cauggcacgc gggagcggac     1260

accggcacac cccacuggaa caacaaagaa gcccuagugg aguucaaaga cgcccacgca     1320

aaacgccaaa ccguagucgu ucucggaagu caggaagggg ccguccacac agcccuagcc     1380

ggagcccucg aagcagaaau ggacggugcg aaagggcgac ucuccucugg acaccucaaa     1440

ugccgcuuaa aaauggacaa acuacggcuc aaaggagucu ccuacagccu cugcacggcc     1500

gccuucaccu ucacaaaaau ccccgccgaa acacuccacg gaacagucac aguagaaguc     1560

caauacgccg gaaccgacgg ccccugcaaa gucccagcac aaauggcagu cgacaugcaa     1620

acacucacac ccguaggcag acucaucacc gccaacccag ucauaacaga aucaacagaa     1680

aauuccaaga ugaugcugga acucgauccc ccauucggcg acucauauau cgucauagga     1740

gugggagaaa agaaaaucac acaccacugg caucgcucag gaagcaccau cggcaaagca     1800

uucgaagcua ccguccgagg agcgaaacgg auggccguac uaggcgacac ggccugggac     1860

uucggcuccg uaggcggagc ccucaauucc cuagggaaag gcauccacca aaucuucggc     1920

gccgccuuca aaucccuauu cggcggcaug uccugguuca gccaaauacu aaucggaaca     1980

cuacucaugu ggcuaggacu caacaccaaa aacggcucca ucagccucau gugccuagcu     2040

cugggcggag uccucaucuu ccucagcacc gcuguauccg ccgacgucgg cugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  5007
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5007
gggagaaagc uuaccaugac cagcgucggu aucguaggcc uacuacuaac gaccgcaaug       60

gcugccgaag uaacaagacg cggcagcgcg uacuacaugu accucgaccg aaacgacgca      120

ggcgaagcaa ucagcuuccc gaccacacua ggcaugaaca aaugcuacau acaaauaaug      180

gaccucgguc auaugugcga cgcaacaaug aguuacgaau gcccaaugcu cgacgaagga      240

guagagcccg acgacgucga cugcuggugc aacaccacca gcacaugggu cgucuacgga      300

acgugccacc acaagaaagg cgaagcccgc agaucccgcc gcgcagucac ccuccccagc      360

cacucaacga gaaaacucca gacccgguca caaacguggc ucgaaucccg agaguacaca      420

aaacaccuca uacgcgugga aaauuggauc uuccgcaacc ccggcuucgc acucgccgca      480

gccgccauag ccuggcuacu cggauccuca acaucccaaa aagucauaua cuuaguaaug      540

auccuacuca uagcaccagc uuacagcauc cgcugcauag gcguauccaa cagagacuuc      600

gucgaaggaa ugagcggcgg aaccugggua gacauagucc uggaacacgg gggaugcgua      660

accgugaugg cacaggacaa acccacaguc gacauagaac ucgugacaac aacgguaagu      720

aacauggcgg aaguucgcag cuacugcuac gaagccucaa ucuccgacau ggcaagcgac      780

ucacggugcc caacgcaggg cgaagccuac cuagacaaac agucagauac acaauacguc      840

ugcaaacgaa cccucguaga ccggggcugg ggcaacggau gcggccucuu cggaaaagga      900

ucacucguca caugcgcaaa guucgcaugc aguaagaaga ugacgggcaa aagcauccag      960

ccagagaacc ucgaauaccg cauuaugcuc ucaguccacg gaucccaaca cucaggcaug     1020

aucgugaacg acacaggaca cgaaacagac gaaaaccgag cgaaaguaga aauaacccca     1080

aacagcccca gagcagaagc aacccucggc ggcuucggca gucucggacu agacugcgaa     1140

ccaagaacag gccucgacuu cagcgaucug uacuaccuaa cgaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccacucccgu ggcacgcagg agcagacacg     1260

ggcacacccc auuggaacaa caaagaggcc cuagucgagu ucaaggacgc ccacgccaaa     1320

cggcagaccg ucgugguacu aggcucacaa gaaggagcag ugcacacagc acuagcagga     1380

gcacuugagg cagaaaugga cggcgccaaa ggucgacugu caagcgggca ccuaaaaugu     1440

cgccuaaaaa uggacaaacu acgccucaaa ggcguaagcu auagccucug caccgcagcg     1500

uucacguuca cgaaaauacc ggcagagacc cuacacggca caguaacggu ugaaguccaa     1560

uacgccggca ccgacgggcc augcaaaguc ccggcccaaa uggccgucga caugcaaacg     1620

cuaacaccgg ucggacgccu caucacagcc aacccgguca ucacagaaag cacagagaac     1680

aguaaaauga ugcuagaacu agacccaccc uucggcgacu cauacaucgu aauaggcgua     1740

ggagagaaga aaauaaccca ccacuggcac agaagcggca guaccauagg caaagcauuc     1800

gaagcaaccg uaagaggcgc caaacgcaug gcaguacuag gcgacaccgc cugggacuuc     1860

ggcagcguag gaggagcccu aaacucccuc ggcaaaggca uacaccaaau auucggcgca     1920

gccuucaaaa gccucuucgg cgggaugagc ugguucuccc agauacuaau aggcacccua     1980

cuuauguggc uaggacuaaa caccaaaaac ggaagcauca gccucaugug ccucgcacuc     2040

ggcggaguac uaaucuuccu cuccacagcg gucucagcag acgucggcug uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  5008
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5008
gggagaaagc uuaccaugac cucaguugga aucgucggcc uacuucugac aaccgccaug       60

gcagccgccg ucacacgcag gggcucagca uauuacaugu accuggaccg aaacgacgcc      120

ggggaggcca ucuccuuccc caccacccuc gggaugaaca aaugcuacau ccaaauaaug      180

gaccugggcc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgaggga      240

guagaacccg acgaugucga cugcuggugc aacaccacga gcaccugggu ggucuacgga      300

acgugccacc acaagaaagg cgaagcccga cgcucccgca gggcggugac ccuacccagc      360

cacagcacac ggaaacuaca aaccaggucc cagacauggc ucgaguccag agaauacacc      420

aaacaccuaa uucgcgucga aaacuggauc uucagaaacc caggauucgc ccuagccgca      480

gccgccaucg cauggcuccu cggcucaagc acaagccaaa aagucaucua ccuagucaug      540

auacugcuaa ucgcccccgc cuacucaauc cgcugcaucg gaguaucaaa ucgcgauuuc      600

guugagggaa ugagcggagg caccuggguc gacaucgucc ucgaacacgg cggcugcgua      660

acagucaugg cucaagacaa accaacagua gacaucgagc uaguaacaac caccguaagc      720

aacauggcag aaguacgguc auacugcuac gaagcaucua uaucagacau ggccucagac      780

ucacgaugcc caacucaagg ggaagcauau cucgacaaac aaagcgacac ccaauaugua      840

ugcaaacgaa cacucguuga cagaggcugg ggaaacggcu guggccucuu cggcaaaggc      900

ucccuuguca caugcgcaaa auucgccugc ucgaagaaaa ugaccggcaa aagcauacaa      960

ccagaaaacc uggaauacag aauaaugcug agcguacacg gcucgcaaca uagcggcaug     1020

auaguaaaug acaccggaca cgaaaccgac gaaaaccgag ccaaaguaga aaucaccccc     1080

aauucucccc gcgccgaagc cacucuggga ggcuucggau cccuaggccu cgacugcgaa     1140

ccaagaaccg gacuagacuu cucagaccua uacuaccuaa caaugaacaa caaacacugg     1200

cuuguccaca aagaaugguu ccacgacauc ccccuacccu ggcacgcagg cgccgacaca     1260

ggaacgcccc acuggaacaa caaagaagca cucguagagu ucaaagacgc ccacgcgaaa     1320

cgccaaaccg uagucguccu gggcucacaa gaaggagcag uccacaccgc gcucgccggu     1380

gcacucgaag cagaaaugga cggcgccaaa ggccgacuaa gcuccggcca ucuaaaaugc     1440

cgucuaaaaa uggacaaacu ccgccuaaaa ggagucagcu acucacugug uacagcagcc     1500

uucaccuuca ccaaaauccc ggccgagacg cuacacggca ccgucaccgu cgaaguucag     1560

uacgcaggga ccgacggacc cugcaaaguc cccgcccaaa uggcgguaga caugcaaacc     1620

cugaccccgg ucggaagacu uauaaccgca aaccccguaa ucaccgaauc aaccgagaac     1680

agcaaaauga ugcuggagcu agacccaccc uucggcgacu ccuacaucgu aaucggcguc     1740

ggcgaaaaga aaauaaccca ccacuggcac cgaucgggau caaccauagg aaaagcguuc     1800

gaagcaaccg uccgcggagc aaaacggaug gccgugcuag gagacacagc augggacuuc     1860

ggaagcguag gaggcgccuu aaacucacua ggcaaaggca uacaccaaau cuucggcgcc     1920

gccuucaaga gcuuauucgg aggcaugagc ugguucuccc aaauccuaau cggcacacuc     1980

cucauguggc ugggacucaa caccaaaaac gggagcauau cgcucaugug ccucgcccug     2040

gguggcgucc uaaucuuccu aucgacagcc gucuccgccg acgucggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  5009
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5009
gggagaaagc uuaccaugcg cggcgccgac accagcguag gcaucgucgg acuacuccuc       60

accacagcca uggcagccgc aguaacacga cgaggaucag ccuacuacau guaccuggac      120

agaaaugacg ccggcgaagc caucagcuuu ccaacaacuc ucgguaugaa caagugcuac      180

auacaaauaa uggaccucgg ccacaugugc gacgcaacaa ugagcuacga augccccaug      240

cuagacgaag gggucgagcc cgacgacgua gacugcuggu gcaacaccac cucaaccugg      300

gugguauacg gaacaugcca ccacaagaaa ggcgaagcuc gacgcagcag acgagccgua      360

acacucccaa gccacagcac aagaaaacuc caaaccagaa gccaaacaug gcucgaaucc      420

agagaauaca caaaacaccu aauuagagua gaaaacugga uauucagaaa ccccgguuuc      480

gcacucgccg ccgccgcaau agcauggcua cucggaagcu ccaccucgca aaaagugauc      540

uaccucguca ugauccuacu aaucgcacca gccuacucca ucagaugcau cggagucucc      600

aaccgagacu ucgucgaagg aaugagcggc ggaaccuggg uagacaucgu gcuagaacac      660

ggcggaugcg ucacuguaau ggcacaagac aaaccaacag ucgacaucga acucguaacc      720

acaacggucu ccaacauggc cgaaguccga uccuacuguu acgaggccag cauaucagau      780

auggcauccg acagccgaug ccccacccaa ggcgaagccu accuagacaa acaaagcgac      840

acccaauacg uaugcaaacg cacccucguu gacagaggcu ggggaaacgg augcgggcua      900

uucggcaagg gcucacucgu cacaugcgcc aaauucgccu gcagcaaaaa gaugaccggg      960

aaaagcauac aacccgaaaa ccuagaauac cgaaucaugc uaucagugca cgggucacag     1020

cauagcggca ugaucgucaa cgacaccgga cacgagacgg acgaaaacag ggccaaaguc     1080

gagaucaccc ccaacucacc gcgcgcagaa gccacgcucg gcggauucgg cucauuagga     1140

cuggauugcg aaccacguac cggccucgac uucuccgacc ucuacuaccu cacaaugaau     1200

aacaaacacu ggcugguaca caaagaaugg uuccacgaca uaccccuacc cuggcacgcc     1260

ggagccgaca caggcacacc gcacuggaac aacaaggaag cccucgucga guucaaagac     1320

gcccacgcua aaagacaaac cgucgucgua cuaggaagcc aagaaggcgc agugcacacc     1380

gcgcuggcgg gcgcacuuga agccgaaaug gauggagcaa aaggaagacu aagcuccgga     1440

caccugaaau gucgccuaaa gauggacaaa cuaaggcuaa aaggcguauc guacucacuc     1500

ugcacagcag cauucacuuu cacaaaaaua cccgccgaaa cgcuccacgg caccguaaca     1560

gucgaagucc aauacgcggg aaccgacggc ccaugcaaag uccccgccca aauggccgug     1620

gacaugcaga cccuaacccc ggugggccgc cuaauaacag caaaccccgu aaucacugaa     1680

agcaccgaaa auucaaaaau gaugcucgaa cucgacccac ccuucggaga cagcuacaua     1740

gugauaggag uaggagagaa aaagauuacc caccauuggc acagaucagg cagcacaauc     1800

ggaaaggcuu ucgaagcaac cguacgcgga gcaaaacgca uggcgguacu cggcgacaca     1860

gccugggacu ucggcagcgu cggcggcgca cuaaacuccu uaggaaaggg uauccaccaa     1920

aucuuuggcg cagccuucaa aagccucuuc ggcggcauga gcugguucuc acaaauccuc     1980

auaggcacac uccucaugug gcucggcuua aacaccaaaa acggauccau cagccuaaug     2040

ugccuggcac ucggcggcgu ccuaaucuuu cuaucaacug ccgucagugc agacguagga     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  5010
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5010
gggagaaagc uuaccaugcg aggccucagc uccagaaaac gacgaucaca cgacguccuc       60

acgguacaau uccucauacu agguaugcua cucaugaccg gaggagugac acgcagaggc      120

agcgccuacu acauguaccu cgaccgcaac gacgcaggag aggccaucag cuuccccaca      180

acccuaggca ugaauaaaug cuacauccaa aucauggacc uaggccacau gugcgacgcc      240

acaaugucau acgaaugccc caugcuugac gaaggaguag aacccgacga cguagacugc      300

uggugcaaca caaccagcac cugggucgua uacggaacgu gccaccacaa gaaaggugaa      360

gcacgaagaa gccgaagggc agucacccuu cccagccacu caaccagaaa acuccaaaca      420

cgaucccaaa ccuggcuaga gagccgcgag uauacgaaac accucauccg cgucgaaaac      480

uggaucuuca gaaacccagg cuucgcgcuc gcugcagcag caauagcaug gcuccuggga      540

agcagcaccu cgcaaaaggu uauauaccua gucaugauac uacugauagc acccgccuac      600

agcauacgcu guaucggagu auccaacaga gacuucgucg aaggaauguc cggaggaaca      660

uggguagaua uagugcucga acacgguggg ugcguaaccg ucauggccca ggacaaaccc      720

accgucgaca uagaacucgu cacaaccacg guaucaaaca uggccgaagu acgaucauac      780

ugcuacgaag caagcauuag cgacauggcg uccgacucaa ggugcccaac ccaaggagaa      840

gcauaccuag acaaacaaag cgacacgcaa uacgugugca agcgaacccu uguagacaga      900

ggauggggga acggaugcgg ccucuucgga aaaggcagcc uaguaacaug cgcaaaauuc      960

gcaugcagca agaaaaugac cggaaaaucg auacaaccag agaaccucga auacagaaua     1020

augcucagcg uccacgguuc acagcacagc ggcaugauag ugaacgacac agggcacgag     1080

accgacgaaa aucgcgcuaa agucgagaua accccaaaca gcccacgagc agaagccacc     1140

cucgggggcu ucggaucacu uggacuagac ugcgaaccac gaaccggacu agauuucagc     1200

gaccucuacu accuaacaau gaacaacaaa cacuggcucg uacacaaaga augguuccac     1260

gauaucccgu uaccauggca cgcaggagcc gacacaggaa cuccccacug gaacaacaaa     1320

gaagcacucg uggaguucaa ggacgcucac gcaaaacgcc aaaccguggu gguccuagga     1380

agccaggaag gagccguuca cacagccuua gccggagcac uagaagcgga gauggacggg     1440

gccaagggcc gccugagcag uggacaccuc aagugccgac ugaaaaugga caaacuaaga     1500

cuaaaaggag ucucguacag ccuaugcaca gcagccuuca ccuucacaaa gauaccggcc     1560

gaaacacuac acggcacagu gacaguagaa guacaauacg caggaaccga cggccccugc     1620

aaaguacccg cccaaauggc aguagacaug caaacccuca cacccguagg caggcuaauc     1680

acggcgaacc caguuauaac ggaaagcacg gagaacagca aaaugaugcu agagcuggac     1740

ccaccauuug gagacucgua cauaguaauc ggagucggag aaaagaaaau cacacaccac     1800

uggcaccguu ccggcagcac gauaggcaag gcguucgaag caacagucag aggcgccaag     1860

aggauggcgg uacucggaga caccgccugg gacuucggcu cagugggcgg cgcgcuaaac     1920

ucacucggaa aaggcauaca ccaaauauuc ggagccgcau ucaaaagucu uuucggaggc     1980

augagcuggu ucagccaaau ccugauaggc accuuacuaa uguggcucgg cuuaaacacc     2040

aaaaacggaa guaucucacu caugugcuua gcccugggcg gaguucucau auuccuuagc     2100

accgcaguca gcgccgacgu ugggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  5011
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5011
gggagaaagc uuaccaugcg cggccucucu agccgcaaac gcagaagcca cgacgucuua       60

acuguacaau ucuuaauccu cggcaugcug cuaaugacag gggcaguaac acgccgagga      120

uccgcuuacu auauguaccu ggaccggaac gacgcaggcg aagccauauc cuucccaaca      180

acccugggca ugaacaaaug cuacauacaa auaauggacc uaggucacau gugcgacgcg      240

accaugagcu acgagugccc caugcucgau gaaggagucg aacccgacga cgucgacugc      300

uggugcaaca ccacgucuac cuggguaguc uacggaaccu gccaccacaa aaagggagaa      360

gcacggcgau cuaggcgagc aguaacgcua ccaucacaua gcacgcggaa acuccagaca      420

cgaucccaaa ccugguuaga aagccgagag uacaccaaac accugauaag aguagaaaac      480

uggaucuuca gaaacccagg auucgcucua gcggcagcag ccaucgccug gcuccuugga      540

ucaucaacau cucaaaaagu aaucuaccuc guaaugaucc uccucaucgc accggcauac      600

agcauacgcu gcaucggcgu cucaaacaga gacuucguag aagggaugag cggcggcaca      660

uggguagaca uaguacucga acacggcggc ugcguaaccg uaauggcgca ggacaaacca      720

accgucgaca ucgagcuagu cacgaccacc gucucaaaca uggcugaagu ccgaucauac      780

ugcuacgaag caagcaucag cgauauggcc uccgacagua gaugccccac ccaaggagaa      840

gccuaccuug acaaacaauc cgacacccaa uacguaugca agcgaacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccuauucggc aaaggcagcc ucgucacaug cgccaaauuc      960

gccugcagca agaaaaugac cggaaaauca auacaaccag aaaaccucga auaccgaauc     1020

augcucagcg uccacggcag ccaacacucc ggcaugauag ucaacgacac aggccacgaa     1080

acagacgaga accgugccaa aguagagaua acacccaaca guccccgugc cgaagcaacc     1140

cucggcggcu ucggcucccu aggccuggac ugcgaaccac gaaccggacu agacuucagc     1200

gaccuauacu accuaacaau gaacaacaag cacuggcugg uacacaaaga augguuccac     1260

gacauccccc uccccuggca cgcgggcgcc gacacgggaa ccccacacug gaacaacaaa     1320

gaagcccucg ucgaguucaa agacgcgcac gcaaaaagac aaacagucgu cguauuaggc     1380

ucacaagaag gcgcaguaca cacagcccua gcuggagccc ucgaagcaga aauggacggg     1440

gcaaaaggcc gacucagcag cggacaccuc aaaugccgcc uuaaaaugga caaacuacga     1500

cuaaagggag ucagcuacuc acucugcacc gccgcauuca cguucaccaa aauccccgcc     1560

gaaacccucc acggaaccgu aacaguagaa guacaauacg caggcaccga cggucccugc     1620

aaagucccag cccaaauggc aguagacaug cagacacuaa cgccuguagg gagacuaauu     1680

acagcaaacc cugucaucac ggaaucaacc gagaacagca aaaugaugcu agaacuagau     1740

cccccauucg gagacaguua caucguaauu ggagucggag aaaagaagau aacgcaccac     1800

uggcacagaa gcggcucaac aauaggaaaa gcauucgaag ccacaguccg gggagccaag     1860

agaauggccg uacuggggga caccgcaugg gacuucggaa gcgugggcgg agcacuaaac     1920

agccuaggca aagggauaca ccaaauauuc ggcgccgccu ucaaaucacu cuucggaggc     1980

augagcuggu ucagccagau ucuuaucggc acacuacuaa uguggcucgg ccugaacacc     2040

aaaaacggua gcauaucacu caugugccuu gcccucggag guguacuaau cuuccugagc     2100

accgccguca gcgcagacgu aggaugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  5012
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5012
gggagaaagc uuaccaugcg aauaauaaac gcacgaaaag agaagaaaag aagaggcgcc       60

gacacaagcg ugggaauagu cggccuacug cuaacaacag ccauggccgc cgaagucacg      120

cgucgaggau ccgccuacua cauguaccuc gaccgaaacg acgccggcga agccauaucc      180

uucccaacaa cccucggcau gaacaaaugc uacauccaaa uaauggaccu gggacacaug      240

ugcgacgcca ccaugucaua cgaaugccca augcuagacg aaggcgucga accagacgau      300

gucgacugcu ggugcaacac aacuagcaca uggguaguau acggcacaug ccaccacaag      360

aaaggggaag cccgccgcag ccggagggcc gucaccuuac caucccacuc aacgcgcaaa      420

cuccaaaccc gaagccaaac auggcucgaa ucgcgagaau acaccaaaca ccucauccgg      480

gucgagaacu ggaucuuucg aaaccccggg uucgcccuag cggcggcggc aauagcaugg      540

cuacucggcu caucaacguc ucaaaaaguc auuuaucuag ucaugauacu gcuaaucgcc      600

ccagcguacu caauacggug caucggagua ucaaaccgcg auuucgugga gggcauguca      660

ggcggaaccu ggguagacau agugcuagag cacggaggcu guguuacugu cauggcccag      720

gacaaaccca ccguggacau cgagcuagua acaacaaccg uauccaacau ggcagaaguc      780

cguucauacu gcuaugaagc cuccaucucc gacauggcau cagauagcag augcccaaca      840

cagggcgaag cauacuuaga caaacaguca gacacgcaau acguuuguaa acgcaccuua      900

gucgacagag gcugggggaa cggaugcggg cuauucggca agggcucgcu aguaaccugc      960

gccaaauucg caugcagcaa gaagaugacc ggcaaaucca uacaaccaga aaaccucgaa     1020

uacagaauaa ugcuaucugu ccacggcagc caacacagcg gaaugauugu caacgacaca     1080

ggccacgaaa cggacgaaaa ccgagccaaa guagaaauca cacccaacuc accgcgagcc     1140

gaagcaaccc ucgggggcuu cggcucccua ggacucgacu gcgaaccccg gaccggccuc     1200

gacuucagcg accucuacua ccuaaccaug aacaacaaac auuggcuagu ccacaaagaa     1260

ugguuccacg acaucccccu uccauggcau gcaggagcag acacaggcac accccacugg     1320

aacaacaaag aagcgcuagu agaguucaaa gacgcacacg caaaacgaca aacagucguc     1380

gugcugggau cccaagaagg cgcuguccau acagcacucg caggcgcacu cgaagccgag     1440

auggauggcg caaaaggacg acuaagcucc ggacaccuca aaugcagacu gaaaauggac     1500

aaacucagac uaaaaggagu cucauacucc cuaugcaccg ccgcguucac cuucaccaaa     1560

auaccagcag aaacacucca cggaacgguc accgucgaag uacaauaugc aggcacagac     1620

ggaccgugca aagucccggc ccaaauggca gucgauaugc aaacacuaac cccagucggc     1680

cgccugauca cugcaaaccc cguaaucaca gaaagcacag aaaacuccaa aaugaugcuc     1740

gaauuggacc cacccuucgg ggauagcuac aucguaaucg gcgucggcga aaagaaaaua     1800

acacaccacu ggcaccgcag ugggagcacu auaggcaaag cauucgaagc caccguucgc     1860

ggagccaaac gcauggccgu acucggcgac acagccuggg auuucggcuc aguaggcggc     1920

gcccuaaacu cccuagggaa aggcauccau caaauauucg gagcugcguu caaaagccua     1980

uucggaggaa ugagcugguu cucccaaaua cucaucggca cacuacugau guggcucggc     2040

cuaaacacca aaaacggauc cauaagccua augugccucg cacucggcgg cgugcucauc     2100

uuccucagca cagccguaag cgcagacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  5013
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5013
gggagaaagc uuaccaugau cauaaacgca cgcaaggaaa agaaacgaag aggcgcugac       60

acaagcgucg ggauaguagg ccuccugcua accaccgcaa uggcagcaga agugacucgc      120

cguggcagcg cauacuacau guaccucgac cguaacgacg caggggaagc aauaaguuuc      180

cccaccacac ucggcaugaa caaaugcuac auccaaauca uggaccucgg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cucgacgagg gagucgaacc agacgacgua      300

gacugcuggu guaacacgac cucaaccugg gugguauacg gaaccugcca ccauaagaaa      360

ggagaagccc gccgaucccg acgcgcagua acccuccccu cacacuccac ccgaaaacua      420

caaacacgcu cacaaacaug gcuggaauca cgcgaauaca ccaaacaccu caucagagua      480

gagaauugga ucuuccgcaa ccccggcuuc gccuuagcag ccgccgccau agcauggcua      540

cucgggagcu cuaccagcca aaaggucaua uaccuaguaa ugauccugcu aaucgcccca      600

gcauacagca uacgcugcau cggcguaucc aacagagacu ucgucgaagg uauguccggc      660

ggcacauggg ucgacaucgu ccucgaacac ggaggcugcg ugaccgucau ggcccaagau      720

aaaccaaccg ucgacauaga acucgucacc accacaguau caaacauggc cgagguacgc      780

ucauacugcu acgaagcauc cauauccgac auggcaagcg acucgaggug cccuacacag      840

ggcgaagccu accuagacaa acaaucagac acacaauacg ucuguaaacg cacccuaguc      900

gacagaggau ggggaaacgg gugcggccua uucgggaagg gcagccuggu aaccugcgca      960

aaauucgccu gcagcaagaa aaugacugga aaaucgaucc aaccugaaaa ccuggaauac     1020

cgaaucaugc uaagcgucca cggaagccag cacucgggca ugaucgucaa ugacacagga     1080

cacgaaacag acgaaaaucg cgcaaaagua gaaauaaccc ccaacucgcc cagggccgaa     1140

gccacgcucg gcggcuucgg aucgcuaggc cuugacugcg agcccagaac cggacuagac     1200

uucucagauc uguacuaccu aaccaugaac aacaaacacu ggcucgugca caaagaaugg     1260

uuucacgaca uaccccuccc auggcacgcc ggcgcagaca caggcacacc ccacuggaac     1320

aacaaagaag cacucguaga guucaaagac gcccacgcaa aacgccaaac agucgucgug     1380

cuaggcuccc aagaaggagc cguccacaca gcccucgccg gcgcccuaga agccgaaaug     1440

gacggagcca aaggacgacu cucaagcggg caucucaaau gcaggcuaaa aauggauaag     1500

cucagacuaa aaggagucag cuacucacua ugcaccgccg ccuucaccuu caccaaaaua     1560

cccgcggaaa cacuacacgg aacaguaacc gucgaagucc aauacgccgg caccgauggc     1620

ccgugcaaag uccccgcuca aauggccguc gacaugcaaa cauuaacgcc cguaggccgc     1680

cucaucacag caaauccagu cauaacggaa agcacagaaa acagcaaaau gaugcucgaa     1740

cucgacccgc ccuucggaga caguuacauc gucauuggcg uaggcgaaaa gaaaauaaca     1800

caccacuggc acagaagcgg aucgaccaua ggcaaagcau ucgaagcaac cguccgaggc     1860

gccaaaagaa uggccguacu cggagauaca gcaugggacu ucggaucagu cggcggagcc     1920

cuaaacuccc ucggcaaagg uauacaccaa aucuucggag ccgccuucaa aagccucuuc     1980

ggaggcaugu cgugguucag ucaaauacuc auaggaacac uacucaugug gcuaggccua     2040

aacaccaaaa acggcucuau cagccucaug ugccuagcac uaggaggcgu ccuaaucuuc     2100

cucagcacag cgguauccgc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  5014
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5014
gggagaaagc uuaccaugau caacgcccgg aaagaaaaga aaaggagagg cgcagacaca       60

agcgucggca uagucggacu ccuacucaca accgccaugg cggccgaagu cacacggaga      120

ggaucggccu acuacaugua ccuagaccga aacgacgccg gagaagccau cagcuucccc      180

acaacacuag gaaugaacaa augcuacaua caaauaaugg accuuggcca uaugugcgac      240

gccacaauga gcuaugaaug ccccaugcuc gacgaaggag ucgagccgga cgacgucgau      300

ugcuggugca acaccacauc cacauggguc gucuacggca caugccacca caagaaagga      360

gaagccagac gcagcagacg agccguaaca cuacccagcc acucaacacg caaacuccaa      420

acccgaagcc aaaccuggcu cgagagcaga gaauauacca aacaccuaau cagggucgag      480

aacuggauau ucaggaaccc aggauucgca cuagcugccg cagcaauagc auggcuacua      540

ggcagcagca ccagccagaa agugaucuac cucguaauga uacuucucau agcacccgcc      600

uacucaauac gaugcauagg cgucagcaac cgcgacuucg ucgaaggcau gucaggugga      660

acauggguag acaucgugcu cgaacacggc ggcugcguca ccgucauggc ccaagacaaa      720

cccaccguag acauagagcu agugaccaca acaguaagca auauggccga agugagaagc      780

uacuguuacg aagccagcau cagcgacaug gcguccgacu cucgcugccc aacccaagga      840

gaagcguacc uagacaaaca aagcgacaca caauacguau gcaaaaggac ccucgucgac      900

cgaggcuggg gcaacggaug cggacuguuc ggcaaaggga gccuaguaac augcgcaaaa      960

uucgcgugcu ccaagaaaau gacaggaaaa agcauacaac cagagaaccu agaauaccgc     1020

aucaugcuaa gcguccacgg aagccaacac ucaggcauga uaguuaacga cacaggccac     1080

gagaccgaug agaaccgcgc caaaguggag aucacaccaa acuccccaag agcagaagcg     1140

acccuaggag gauucggauc gcucgggcuc gacugcgagc caagaacagg ccuagacuuc     1200

uccgaccucu acuaccuaac caugaacaau aaacauuggc uagugcacaa agaaugguuc     1260

cacgacauac cgcucccaug gcacgccggc gcugacacgg gaacgcccca cuggaacaau     1320

aaagaagcac uggucgaguu caaggacgca cacgccaaaa gacaaaccgu cguaguacuc     1380

ggaagccaag aaggcgccgu acacaccgca cuggccggag cccuagaagc agaaauggac     1440

ggcgcgaaag gaagacucag cucaggacac cugaagugcc ggcucaaaau ggacaaacuc     1500

cgacucaaag gaguaagcua cagccucugc acagccgccu ucaccuucac caagaucccg     1560

gcagaaaccc uucacggcac cgucacagua gaaguacaau acgcagguac agacggaccc     1620

ugcaaggucc ccgcacaaau ggcaguagac augcaaaccc ugacaccagu ugggagacug     1680

aucaccgcaa acccagugau caccgaauca accgaaaaca gcaaaaugau gcuagaacuc     1740

gacccaccau ucggggacag cuacauagua aucggcgugg gagaaaagaa aauaacacac     1800

cacuggcacc gcucaggcag uacuauagga aaagccuucg aggccacagu ccguggggcc     1860

aaaagaaugg cgguacuagg cgacaccgcc ugggacuucg gcagcguagg gggcgcacua     1920

aacucacucg gaaaagggau ucaccagauu uucggcgcag ccuucaaaag ccucuucgga     1980

ggaaugagcu gguucagcca aauacucaua ggaacgcuac ugauguggcu gggccucaac     2040

accaaaaacg ggucaauaag ccucaugugc cuagcccuag gcggaguccu aauauuccua     2100

uccacagcag ucagcgcaga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  5015
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5015
gggagaaagc uuaccaugaa ugcacgaaaa gaaaagaaac gacgaggagc agacacuuca       60

gucggcaucg ugggccugcu acucacaaca gcgauggccg ccgaagucac acgccgaggc      120

ucugcuuacu acauguaccu cgaucgaaac gacgccgggg aagcaaucuc guuccccacc      180

acgcugggaa ugaacaaaug uuacauacaa aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgaaugccc aaugcucgac gaaggaguag agccagacga cgucgacugc      300

uggugcaaca ccaccagcac guggguaguc uacgggacgu gccaccauaa gaaaggagaa      360

gcacgcagaa guagacgagc agucacccua ccaagccacu caacccgcaa acuacaaacc      420

agaucucaaa cguggcucga aagucgcgag uacacuaaac accucauacg cgucgaaaac      480

uggaucuucc gaaaucccgg auucgcgcug gcagcagcag ccauagccug guuacugggc      540

agcuccacca gccaaaaagu aaucuaccuu gugaugaucc uacuaaucgc uccagccuac      600

agcauccgau gcauaggcgu cagcaaccga gacuucgucg agggcauguc cggcggaacc      660

uggguagaca ucguacuaga acacggcggg ugcgucacag ucauggcaca agacaaaccc      720

accgucgaca uagagcuagu aaccacgaca guaagcaaca uggcagaagu cagaagcuac      780

uguuacgaag caucaauauc cgacauggca agcgacuccc gaugccccac ccaaggcgaa      840

gcauaccuag acaaacaaag cgacacccag uacgucugca aaaggacccu agucgaccga      900

ggcuggggaa acgggugcgg acuguucggg aaaggcagcc ucguaaccug cgccaaauuc      960

gccugcucaa agaaaaugac gggcaaaagc auacaacccg aaaaccucga auacaggauc     1020

augcuaucug uccauggcag ccaacacucc ggcaugauag ucaaugauac uggacacgaa     1080

acagacgaaa accgcgccaa aguagaaauc acacccaaca gcccccgcgc agaagcaacc     1140

cucggaggcu ucggaucccu aggccucgac ugcgaaccac gcaccggccu cgacuucucc     1200

gaucucuacu accucaccau gaacaauaaa cacuggcugg uccacaaaga augguuccac     1260

gacauacccc ugccguggca cgccggcgcc gacacaggaa ccccacacug gaacaacaaa     1320

gaggcgcuag ucgaguucaa agacgcccac gcuaaacgac aaacggucgu cguccuaggc     1380

ucccaagaag gggcggucca cacagcccuc gcaggagccc uggaagccga aauggacggc     1440

gccaaaggca gacucagcuc cggccaccua aagugccgcc ucaaaaugga caaacuaaga     1500

cugaaaggcg ucagcuacag ccuaugcaca gcagcauuca cauucaccaa aauacccgcc     1560

gaaacccugc acggaacagu gacaguagaa guccaauacg ccggcaccga cgggcccugc     1620

aaaguaccag cccaaauggc cgucgacaug caaacgcuca ccccagucgg ccgacugauc     1680

accgccaacc cagucaucac cgaauccaca gagaacucaa aaaugaugcu cgaacuagau     1740

cccccauucg gcgacuccua cauaguuauc ggcguuggag aaaagaaaau cacgcaccac     1800

uggcacagga gcgguucaac aauaggaaaa gccuucgaag cgacaguccg cggagccaaa     1860

cgaauggcgg uacugggcga cacugcaugg gacuucggca gcguaggcgg cgcucucaac     1920

ucccugggaa aaggaauaca ucaaauauuc ggagccgcau uuaaaucacu guucggaggc     1980

augucauggu ucagccagau ccuaauaggc acccuccuca uguggcucgg acuaaacacg     2040

aaaaauggaa gcauaagccu uaugugccua gcacucggcg gaguccuaau auuccucagc     2100

acggccguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  5016
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5016
gggagaaagc uuaccauggc ccgaaaagaa aaaaaacgac gaggcgccga cacauccgua       60

ggaauagucg gccuccuacu aaccacggcu auggcagccg aaguaacgcg ccgaggauca      120

gccuacuaca uguacuuaga cagaaacgac gcaggagaag caaucagcuu cccaaccacg      180

cucggaauga auaaaugcua cauccaaaua auggaccuag gccacaugug ugacgccacu      240

auguccuacg aaugccccau gcuagacgag ggcgucgagc cagacgacgu cgauugcugg      300

ugcaacacca ccagcaccug ggucguguac gggacaugcc accacaaaaa aggcgaagca      360

cgacgauccc gccgagcagu cacccugcca ucccauagca caagaaaacu ccaaacccgc      420

agccagacau ggcuagaaag ccgagaauac acaaaacacc ucauccgagu ggaaaacugg      480

aucuuucgca acccaggcuu cgcacucgca gcagccgcca ucgccuggcu ccuaggcucg      540

agcaccagcc aaaaagucau auaccucguc augauacuac ucauagcccc ggcauacucc      600

auccgaugca uuggagucag caaccgggac uucgucgaag gaaugagcgg cggcaccugg      660

guagacaucg ugcucgaaca cggaggcugc guaaccguca uggcgcagga caaacccaca      720

guagauaucg aacuagucac cacuacagua agcaacaugg cggaaguacg aagcuacugu      780

uacgaggcca gcauauccga cauggcaucg gacagcagau gccccaccca aggggaggca      840

uaccucgaca aacaaucaga cacgcaauac guaugcaaac ggacccuggu cgaucgcgga      900

uggggcaacg ggugcggccu cuucggcaaa ggcucacucg ucaccugcgc caaauucgca      960

ugcuccaaga aaaugaccgg caaaagcaua caaccagaga accuagaaua ccgaaucaug     1020

cugucagucc acgguagcca acacagcggc augaucguca acgacaccgg acacgaaacc     1080

gacgaaaaua gagccaaagu agaaaucacg cccaacagcc caagagcaga agccacccuc     1140

ggcggguucg guagccuagg ccuugacugc gagccgcgca caggacugga cuucagcgac     1200

cucuacuacc ugaccaugaa caauaaacac uggcucguac acaaagagug guuccacgac     1260

aucccccuac ccuggcacgc aggagccgac accggcacgc cacauuggaa caacaaagaa     1320

gcccucgucg aauucaaaga cgcgcaugcg aaaagacaga cagucguagu ccuaggauca     1380

caagaaggag cgguacauac agcacuagca ggagcgcucg aagcagaaau ggacggcgca     1440

aaaggacgac uaucgucagg ccaccucaaa ugccgacuca aaauggacaa acuaagacuc     1500

aaagggguca gcuacuccuu augcaccgcc gcauucacau ucaccaaaau ccccgcagaa     1560

accuuacacg gaaccgugac cgucgaagua caauacgcag gaaccgaugg acccugcaaa     1620

gucccagcac agauggccgu agauaugcaa acccugacac cggucggccg acuaauaaca     1680

gcaaaccccg uaaucacuga aucaaccgaa aacucaaaaa ugaugcuaga acuugaccca     1740

cccuucggcg acagcuacau cguaaucggc guaggcgaga aaaaaauuac acaccacugg     1800

caccgauccg gaagcacgau cggcaaggca uucgaagcga cgguccgagg agccaaacga     1860

auggcagucc ucggcgacac agcgugggac uucggcagcg ucggcggcgc ucucaacagc     1920

cuaggcaaag gcauccacca gaucuucggc gccgccuuca aaucccuauu cggcggcaug     1980

ucaugguuca gccaaauccu aauaggcaca cuccucaugu ggcucggccu caacacaaaa     2040

aacggcucaa uaagccuaau gugccucgcc cucggcggcg uucucauuuu ccucagcacc     2100

gcagucagcg cggacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  5017
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5017
gggagaaagc uuaccaugcg gaaagaaaag aaacgccgag gcgcagacac gagcguagga       60

auagucggcc uacuccugac gacagcaaug gcagcagaag uaacacgccg uggaagcgcg      120

uacuacaugu accucgaccg gaacgacgca ggagaagcca ucagcuuccc aaccacccuc      180

ggaaugaaca aaugcuacau acaaauaaug gaccuaggac acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu agacgaagga guagaaccag acgacguaga cugcuggugc      300

aacacaacau ccacgugggu aguauacgga acaugccacc acaagaaagg cgaagcacgc      360

cggagccgac gagccgucac gcugccaucc cacagcacac gaaaacuaca aacccggucc      420

cagaccuggc uagaaucaag agaauacaca aaacaccuca uacggguaga gaacuggaua      480

uucagaaacc ccggguucgc acucgcagcc gccgcaauag ccuggcuccu gggcucaagc      540

accucccaaa aagugauaua ccuagucaug auacugcuga ucgccccggc cuacuccauc      600

agaugcaucg gggucagcaa cagagacuuc gucgaaggua ugucuggagg aaccugggua      660

gacauaguac uagaacacgg aggaugcguc acggucaugg cucaggacaa acccaccgua      720

gauauagaac uggucaccac aacgguaagc aauauggccg aaguaagauc cuacugcuac      780

gaggccagca uaucagacau ggcauccgac ucacgcugcc caacccaagg agaagcauac      840

cucgacaaac aaagcgauac gcaauacgug ugcaaaagaa cccuaguaga ccgaggaugg      900

ggaaacggcu gcggccuguu cggcaaaggg ucacugguaa ccugugcgaa auucgcgugc      960

ucaaagaaaa ugacagggaa auccauccaa cccgaaaacc ucgaguaccg aauaaugcua     1020

agcguccacg ggucacaaca cuccggaaug aucgucaacg acacaggaca cgaaacagac     1080

gaaaacagag caaaaguaga aaucacaccg aacagccccc gugcugaggc aacacucggg     1140

ggauucgggu cccuaggacu cgacugcgaa ccacgaacgg gacuagacuu cagcgaccua     1200

uacuaucuca cgaugaacaa caaacacugg cuagugcaca aagaaugguu ccacgacauc     1260

ccccuacccu ggcacgcagg agccgacacu ggcacacccc acuggaacaa caaagaagca     1320

cugguagagu ucaaagacgc acacgccaag agacagaccg uagucguacu cggaagccaa     1380

gagggggcgg ugcacacagc acucgcagga gcgcucgaag cagagaugga cggggccaaa     1440

ggccgacuau ccagcggaca ccucaaaugc cgacuaaaaa uggacaaacu cagacuaaaa     1500

ggagucuccu acagccuaug cacagcagcc uucaccuuca ccaagauacc cgcagaaaca     1560

cuccacggca ccgucaccgu cgaaguacaa uacgccggga ccgauggacc augcaaaguc     1620

ccagcgcaaa uggcagugga caugcaaacc cuaaccccag ucggacgucu gaucaccgca     1680

aaucccguca ucacagaauc caccgaaaau agcaaaauga ugcuggaacu ggacccaccc     1740

uucggcgaca guuacauagu caucggaguc ggagaaaaga aaauaacaca ccauuggcac     1800

agguccggga gcaccauagg caaagcauuc gaagcaacag uacgaggagc caaaagaaug     1860

gccgucuugg gagacacugc augggacuuc ggcuccguag gaggagcacu caacagccuc     1920

ggaaaaggaa uccaccagau auucggcgca gcauucaaau cccucuucgg aggcaugagc     1980

ugguucuccc aaauacuuau aggcacgcuc cuaauguggc uaggccucaa uaccaaaaac     2040

ggaagcaucu cucuaaugug ccucgcgcua ggaggcgucc uaauauuccu aucaacagca     2100

guuucagcag acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  5018
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5018
gggagaaagc uuaccaugaa agaaaagaaa cgccgagggg cggacacaag cguggguauc       60

gucggccuac ucuuaaccac cgcgauggcc gcggagguca cccgacgagg cuccgcguac      120

uacauguacu uagaccgaaa ugacgccggc gaagccauau cauuccccac cacgcucggc      180

augaacaaau gcuacaucca aauaauggac cucggccaca ugugcgacgc caccaugagu      240

uaugaaugcc ccaugcucga cgaaggggua gagccagacg acguagacug uuggugcaac      300

acaaccagca caugggucgu guacggaacc ugccaccaca agaaaggcga agcccggcgc      360

agccgccggg cagucacacu ccccagccau agcacccgca aacuacagac ccgcucgcaa      420

accuggcucg aaucaagaga auacaccaaa caccucaucc gcgucgaaaa cuggauauuu      480

cgaaaccccg gauucgcccu ggcagcagca gcaauagccu ggcuucucgg cuccagcaca      540

agccagaaag ucaucuaccu cguaaugauc cuauuaauag ccccagcuua cuccauccgg      600

uguauagggg ucuccaacag agacuucguc gaaggcaugu ccgguggaac cuggguagac      660

aucguacucg aacacggagg augcgucacc gucauggccc aagacaaacc caccguggac      720

aucgaacucg ucacaaccac agucucgaac auggccgaag ucagguccua cugcuacgaa      780

gcgucaauau cagacauggc auccgacagc cgaugcccaa cccagggaga agcauaccuu      840

gacaaacaau cggacaccca auacgucugc aagcgcacac ucguggacag aggauggggc      900

aacggaugcg gacuauuugg caaagggagc cuaguaaccu gcgccaaauu cgcgugcagc      960

aagaaaauga cuggcaaaag uauccaacca gagaacuuag aauaucgaau caugcucagc     1020

guccacggaa gccaacacuc cggcaugauc gucaacgaca caggccacga gacagacgaa     1080

aaccgugcca aaguagaaau aacaccaaau agcccccgag cagaggcaac ccucggaggc     1140

uucgggagcc uaggacucga cugcgagccg aggacgggac uagauuucag cgaccuauac     1200

uaccuaacaa ugaacaacaa acauuggcuu guccacaaag aaugguucca cgacauccca     1260

cuccccuggc acgccggagc cgacacuggc acaccucacu ggaacaacaa agaagcacuc     1320

guagaguuca aggacgccca cgcuaaacgc caaacaguug uaguacucgg cagccaagaa     1380

ggagccgucc acacagcacu cgcaggcgcc cucgaagccg aaauggaugg agccaagggc     1440

cgacuaucgu caggacaccu caagugccgg cucaagaugg acaaacucag acugaaagga     1500

guaagcuaca gccuaugcac cgccgccuuc accuucacca agauccccgc agaaacacuc     1560

cacggaacag ucaccgucga aguacaguac gccggaaccg acggccccug caaagucccc     1620

gcacaaaugg ccguggacau gcagacccua acaccaguag gacgccucau aacagcaaau     1680

ccaguaauaa ccgagucaac cgaaaacagc aaaaugaugc uagaacuaga uccacccuuc     1740

ggcgacuccu acauaguaau cggcguaggc gagaagaaaa uaacacauca cuggcacaga     1800

ucagguucca caaucgggaa agcauucgaa gccacagucc gcggagccaa aagaauggcg     1860

guacuaggcg acacagccug ggacuucgga aguguaggug gcgcgcucaa cucccuaggg     1920

aagggaauac accaaaucuu cggcgccgca uucaaaagcc uauucggcgg aaugagcugg     1980

uucagccaaa uucucauagg cacccuccua auguggcuug gacucaauac aaagaacggg     2040

agcauauccc uaaugugccu cgcccuaggc ggcguccuaa uauuucugag cacagcaguc     2100

agcgcagacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  5019
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5019
gggagaaagc uuaccaugga aaagaagagg cgaggagccg acaccagcgu agggauagua       60

ggccuacucc ucacaacagc uauggcagca gaagucacac gacgcggcag ugcauacuac      120

auguaccucg accgaaacga ugccggcgag gccaucagcu uccccaccac acuaggcaug      180

aacaaaugcu acauacaaau cauggaccuc ggacacaugu gcgacgcaac aauguccuac      240

gaaugcccaa ugcucgacga ggggguagaa cccgacgacg uagacugcug gugcaacacg      300

accuccacau gggugguuua cggcacaugc caccacaaga aaggcgaagc ccgacgaagc      360

cgacgugcag ugacacuccc aucgcacucc acacgaaaac uacaaacacg aucucaaaca      420

uggcuagaau cccgcgaaua cacaaaacac cuaauacggg uggaaaacug gauauucaga      480

aaccccggcu ucgcccuagc agcagccgca auagcauggc ucuuagggag cuccaccuca      540

caaaaaguca ucuaccucgu aaugaucuua cuaauagcgc cggccuacag cauacgcugc      600

auagggguaa gcaaccgaga cuucgucgaa ggaaugagcg gaggcaccug gguagauauc      660

guacuagagc acggcggcug cgucacugua auggcacaag acaaaccuac aguagacauc      720

gaauugguaa caacaacagu cagcaacaug gccgaaguca gaucauacug cuacgaagcg      780

agcauaagcg acauggcauc ugacagccgc ugccccaccc agggcgaagc cuaccucgac      840

aaacagucag acacccaaua cguaugcaaa agaacacucg uagaccgagg auggggcaau      900

ggaugcggac ucuucgggaa aggaagccua gucacaugcg ccaaauuugc augcucaaag      960

aaaaugacag gaaaaucgau ccaaccagaa aacuuagaau aucgaaucau gcugagcgua     1020

cacggcagcc aacacagcgg caugauaguc aacgacacug gacacgaaac cgacgaaaac     1080

cgggcaaaag ucgaaaucac cccaaacuca ccaagggccg aagcaacacu cggcggcuuu     1140

ggaagccuag gccucgacug cgaaccccgc accggacuag acuucucaga ccuauacuac     1200

cuaaccauga acaacaaaca cuggcuaguu cacaaggaau gguuccacga cauaccccuc     1260

ccauggcacg ccggcgcaga cacaggaacg ccccacugga acaacaaaga agcacucgua     1320

gaguucaagg acgcccacgc caagcggcag acaguagucg ugcucggauc acaagaagga     1380

gccguccaca cagcccucgc cggagcccuu gaagcggaaa uggacggcgc aaaaggacgg     1440

cugucuagug gacaccuaaa augucggcug aaaauggaca aacugcgucu aaaaggugua     1500

agcuacagcc uaugcacagc agcguucacc uucaccaaaa ucccggcaga aacgcuccac     1560

ggaaccguca caguagaagu acaauacgca gggacagacg gcccaugcaa aguacccgcc     1620

caaauggccg uagacaugca aacacuaaca ccagucggaa gacucauaac agccaacccc     1680

guaaucacag aaagcaccga gaacuccaaa augaugcugg aacucgaucc ucccuucggc     1740

gacagcuaua uagucauagg ugucggugag aagaaaauaa cacaccacug gcaccgcagc     1800

ggcagcacaa ucggaaaagc cuucgaagca accguucggg gagcgaaacg aauggccguc     1860

uuaggcgaca cagcauggga cuucggcucu guaggcggag cacugaacag ccuaggcaaa     1920

ggaauccacc agauauucgg cgcagccuuc aaaucacucu ucggcgggau gucuugguuc     1980

ucacaaauac ucauaggaac acuccucaug uggcuaggcu uaaacaccaa aaacggaucg     2040

aucucgcuga ugugccuagc acucggcgga gugcugauau uccuaucgac cgcaguaucg     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  5020
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5020
gggagaaagc uuaccaugaa gaaacgccgc ggcgccgaca ccagcguagg aaucgucgga       60

cuacuguuga caaccgcaau ggccgcggaa gugacacgaa ggggaagcgc cuacuacaug      120

uaccucgaca gaaacgacgc cggagaagcc auauccuucc cgaccacacu aggcaugaac      180

aaaugcuaca uacaaauaau ggacuuaggc cauaugugcg acgcaacgau gucauacgaa      240

ugcccuaugc uagacgaagg aguggaaccc gacgacgucg acugcuggug caacacgaca      300

ucgaccuggg uagucuacgg cacuugccac cacaagaaag gagaagcacg acgaagccga      360

cgggcaguca cgcuacccag ccacuccacc cgaaaacucc aaacucgcag ccaaaccugg      420

cucgaaagcc gcgaauacac caagcaccua auacgagucg aaaacuggau auuccggaac      480

ccaggcuucg cccucgcagc agcagcaaua gccuggcucc uuggaagcuc cacaucccaa      540

aaaguaauau accuagucau gauacuccua aucgcccccg cauacagcau ccgauguauc      600

ggaguaucca accgcgacuu cgucgagggc augagcggcg gcaccugggu agacauaguc      660

cucgaacacg gaggcugcgu cacaguaaug gcccaagaca aacccacagu agacaucgag      720

cuggucacca caacgguaag caacauggcc gaaguccgau ccuacugcua cgaagcaagc      780

auaucagaca uggcaucaga cagcagaugc cccacacaag gcgaagccua ccuagacaaa      840

caaagcgaca cacaauacgu augcaaacgg acguuggucg accggggcug gggcaacgga      900

ugcggucucu ucggaaaagg cagccucgua accugcgcca aauucgcgug cucaaagaaa      960

augacgggca aaucaaucca accggaaaau cuagaguacc ggauaaugcu aagcguccac     1020

ggaucacagc acuccggcau gaucgucaac gacaccggac acgaaacaga cgaaaaccgu     1080

gcaaaagugg agaucacacc aaacagcccc cgcgccgaag ccacacuagg cggauucgga     1140

ucccugggac uagacugcga accaaggaca ggacuagacu ucuccgaccu cuacuaccuc     1200

acaaugaaua acaaacacug gcuaguacac aaagaauggu uccacgacau cccacucccc     1260

uggcacgccg gagcagacac aggcacacca cacuggaaca acaaagaagc acucgucgag     1320

uucaaagacg cacaugccaa acgccaaacc gucguagugc uaggauccca agaaggagca     1380

guccacaccg cgcucgccgg agcccuggaa gccgaaaugg acggagcaaa agggcgccug     1440

aguuccggcc accuaaagug ccgccuaaag auggacaaac uaagacucaa aggcgucucc     1500

uacagccucu gcaccgcagc guucaccuuc acuaaaaucc cggccgaaac acugcacgga     1560

acaguaaccg uggaggugca guacgcaggc acagacggac ccugcaaagu gccggcccaa     1620

auggcagucg auaugcaaac acuaacgccc guaggacgac ucaucacagc caaccccgua     1680

aucacagagu ccacagaaaa cucaaaaaug augcuagaac uagauccccc auucggagac     1740

agcuacauag ucaucggcgu cggugaaaag aaaauaacac accauuggca cagaagcggc     1800

ucaaccaucg gaaaagccuu cgaagccacg guccgagggg caaaacgcau ggccguccua     1860

ggggacacgg caugggacuu cgggagcgua gggggagccc ucaacucccu gggcaaggga     1920

auacaccaaa uauucggcgc cgcuuucaaa agccucuucg gcgggaugag cugguucagc     1980

caaauccuca ucggaacacu ucucaugugg cucggucuaa acaccaaaaa cggaagcaua     2040

ucgcugaugu gccucgcgcu uggcggcguc cuuauauucc uaucaaccgc cguuuccgcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  5021
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5021
gggagaaagc uuaccaugaa gcgccgcgga gccgacacaa gcguaggaau ugucggccuc       60

cuucuuacaa cagcgauggc ggccgaagua acccgaagag gcuccgccua cuauauguac      120

cucgacagga acgacgccgg cgaagcaauc ucauucccca caacccuggg aaugaacaaa      180

ugcuacaucc agauuaugga ccuaggccac augugcgaug ccaccauguc guacgaaugc      240

cccaugcuag acgaaggagu agaaccagac gacguagacu gcuggugcaa cacaaccagc      300

acaugggucg uauacggaac augccaccac aaaaagggcg aagcgcgaag aagccgccgc      360

gcagugaccc ucccaagcca cagcacacga aaacuccaaa cacgcagcca aacauggcuc      420

gaaagccggg aauacaccaa acaccucauc agggucgaaa acuggaucuu ccgaaacccc      480

ggcuucgcac ucgcugcagc agcaauagcc uggcuucucg gauccagcac cucccaaaaa      540

gucauauacc uagucaugau acuccuaaua gcccccgcau acucuauacg cugcauagga      600

gugaguaacc gagacuucgu ggaagggaug uccggcggaa caugggucga caucguccua      660

gaacacggug gcugcguaac aguaauggca caagacaaac ccacagugga caucgagcuu      720

guaacaacca caguauccaa cauggcagaa gucagauccu acugcuacga agccuccaua      780

ucugacaugg ccucagacuc acgaugccca acccaaggag aagcauaccu agacaaacaa      840

agcgacacac aauauguaug caaaagaaca uuagucgacc gcggaugggg aaacggaugc      900

ggacuuuucg gaaaagguuc ccuagugacu ugcgccaaau ucgccuguuc gaagaaaaug      960

accggcaagu ccauccaacc ggaaaaccug gaauaccgaa ucaugcuauc uguccacggc     1020

ucgcaacacu caggcaugau cguaaacgac acgggccacg aaacagacga aaacagagcc     1080

aaagucgaga uaacccccaa cuccccucgc gccgaagcca cgcucggcgg auucggaagc     1140

cugggccucg acugugaacc gcgaacagga cuagacuuca gcgaccucua cuaccuaacc     1200

augaacaaca agcacuggcu aguccacaaa gaaugguucc acgacauccc acucccaugg     1260

cacgcaggag ccgacaccgg aacaccacac uggaacaaca aggaagcccu cgucgaguuc     1320

aaagacgcgc acgccaaacg ccaaacagug gucguccuag gaucccaaga aggcgccguc     1380

cacaccgcac uagcgggagc ccucgaagcc gaaauggacg gcgcaaaagg aagacucagc     1440

ucgggccacc uaaaaugcag acuaaaaaug gauaagcucc gacugaaagg aguaucuuac     1500

ucccuaugca cagcagcauu caccuuuacu aaaauccccg cagaaacacu gcacggaaca     1560

guaaccgucg agguacaaua cgcaggcacc gacggacccu gcaaaguacc ggcccaaaug     1620

gccgucgaca ugcaaacccu cacaccaguc ggaagacuca ucacggcaaa ccccguaauc     1680

accgaaucca ccgaaaacuc uaaaaugaug cuggagcuag acccacccuu cggcgacagc     1740

uacaucguca ucggaguggg agagaagaag aucacccacc acuggcaccg aagcggcagc     1800

accaucggca aagcauucga ggcgacuguc cgcggagcga aaagaauggc cguucucggg     1860

gacaccgcau gggacuucgg guccgucggc ggcgccuuaa acagucucgg aaaaggcaua     1920

caccaaauau ucggcgccgc guucaaaagc cuguucggag gcauguccug guucucgcaa     1980

auacuaaucg gcacccuccu uauguggcuc gggcuaaaca ccaaaaacgg aagcauaagc     2040

cuaaugugcc uagcccuagg cggcgugcuc aucuuccuua gcacagccgu aagcgcggac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  5022
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5022
gggagaaagc uuaccaugag acggggcgca gacacaagcg ucggaaucgu cggacuccua       60

cuuacgaccg caauggcggc ugaagucaca cgccgaggau ccgcauacua cauguaccuc      120

gaccguaacg acgcaggcga agccauauca uucccuacaa cgcugggaau gaacaaaugc      180

uacauccaga uaauggaccu aggccacaug ugcgacgcaa ccauguccua cgaaugccca      240

augcucgacg aaggggucga acccgacgac guagacugcu ggugcaacac aacgagcaca      300

uggguagugu acggaacaug ccaccacaaa aagggggaag cccgacgauc caggagagca      360

gugacacucc caagccacag cacaagaaaa cuacagacaa gaucacaaac cuggcuugaa      420

agcagagaau acacaaagca ccuaauacga guugaaaacu ggauauucag aaaccccggc      480

uucgcacucg cagcagccgc cauagccugg cuccugggca guucaacgag ccaaaaaguc      540

auauaccuag uaaugauacu gcuaaucgcc ccagcauacu caauacgcug caucggcgua      600

uccaaccgag acuucguaga aggaauguca ggaggaaccu ggguagacau uguacucgaa      660

cacggaggau gcguaacagu cauggcccag gacaaaccca cggucgacau cgaacucgua      720

acaacaaccg uaucaaacau ggcagaagua cgauccuacu gcuacgaagc aagcaucucu      780

gacauggccu cagacucacg augccccacc cagggcgaag ccuaccuaga caaacagucg      840

gacacacaau acgucugcaa acgaacacug gucgaccgcg guuggggcaa cggcugcggc      900

cuguucggaa aagggagccu agugaccugc gccaaauucg ccugcagcaa gaaaaugacc      960

ggcaaaucga uacaacccga aaaccuugag uacagaauca ugcuauccgu ccacggcagc     1020

caacauuccg gcaugauagu caacgacacc ggccacgaaa cagacgaaaa cagagcaaaa     1080

gucgaaauca cgcccaacuc cccacgggcg gaagccacac ugggaggauu cggcagccuc     1140

ggccuagauu gcgaaccacg cacaggguua gacuucuccg accucuacua ccuuacaaug     1200

aacaacaaac auuggcucgu gcacaaagaa ugguuccaug acaucccacu ccccuggcau     1260

gcaggcgccg acacggggac accacacugg aauaacaagg aggcgcuugu ggaguucaag     1320

gaugcgcacg ccaaacgaca aacaguaguu guccuaggcu cacaagaagg cgcgguccac     1380

acagcccucg ccggggcacu agaagccgaa auggacggcg ccaaagggcg acucagcagc     1440

ggccaccuca aaugccgacu aaaaauggac aaacugcgcc uaaaaggggu cagcuacagc     1500

cucugcaccg ccgcauucac cuucacaaaa auaccagccg aaacccuaca cggcacaguc     1560

acaguagaag uacaauacgc cggcacagac ggacccugca aagucccagc ccaaauggcg     1620

gucgacaugc aaacccucac gccaguaggc cgacuaauca cagcaaaccc agucaucacc     1680

gagagcacag aaaacagcaa aaugaugcua gaacuggacc cgccauuugg agauaguuac     1740

aucguaauug gcguggggga aaagaaaaua acacaccacu ggcacagaag cggcuccaca     1800

aucggaaaag cauucgaagc uaccguccga ggagcgaaac gaauggccgu acucggcgac     1860

acagccuggg acuucggcuc aguaggcgga gcccugaacu cccucggcaa aggaauacac     1920

caaaucuucg gggccgcguu caaaucccug uucggaggaa ugagcugguu cucacagaua     1980

cucauaggca cacuacuaau guggcuaggc cuaaacacaa agaacggcag cauuagcuug     2040

augugccuag ccuugggagg cguacuaaua uuccuaagca cagccguauc agccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  5023
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5023
gggagaaagc uuaccaugcg aggagccgau acuucggucg gcauagucgg ccuacuccua       60

accacggcca uggcugcaga agucacaaga cggggcucag ccuacuacau guaccucgac      120

agaaacgaug caggagaagc aaucucguuc cccacaacac ugggcaugaa caaaugcuau      180

auacaaauca uggaucuugg ccacaugugc gacgcaacca ugagcuacga gugcccaaug      240

cucgacgaag gcgucgaacc cgaugacgug gacugcuggu gcaacacaac cagcacaugg      300

gucguauacg gaaccugcca ccacaagaaa ggcgaagccc gccgaucacg gcgcgcagug      360

acccuccccu cacacuccac ccgaaaacua caaacaagaa gccaaaccug gcucgaauca      420

cgcgaguaca ccaaacaccu aaucagagua gaaaacugga uauuccgcaa cccaggcuuc      480

gcacuggccg ccgcggcaau agcauggcuc cuuggcucaa gcacaucaca aaaaguaauu      540

uaccucguaa ugauacuacu caucgccccc gccuauagca uccgcugcau cggcgucuca      600

aaucgcgacu ucguagaagg caugagcggc ggaacauggg uagacaucgu ccucgaacac      660

ggagguugcg ucacggucau ggcccaagac aaacccacag uagacaucga gcuagucacc      720

acgacagucu cuaacauggc ugaaguacga agcuauugcu acgaagcgag uaucuccgac      780

auggcaucag acucacguug ccccacccaa ggagaggccu accucgacaa acaauccgac      840

acacaauacg ucugcaaacg cacccucgua gacagaggau gggggaacgg cugcggacug      900

uucggcaaag gaagccucgu aacaugcgca aaauucgcau gcucaaagaa aaugaccgga      960

aaaucaauac aaccagaaaa ccucgaauac cgcauaaugc uauccguaca cggcucccaa     1020

cacuccggaa ugauaguaaa cgacaccggc cacgaaaccg acgagaaccg agcaaaaguc     1080

gagaucaccc cuaacucccc acgcgcagaa gcaacccuag gcggcuucgg cucccucgga     1140

cuggacugcg aacccagaac cggacuggac uucucagacc ucuacuaccu cacaaugaac     1200

aacaaacacu ggcucguuca caaggagugg uuccacgaca ucccccuccc cuggcacgca     1260

ggcgcggaca cagguacgcc acacuggaac aauaaggagg cacucgucga guucaaagac     1320

gcacacgcaa aacgccaaac ugucgucgua cugggcuccc aagaaggagc cguacacaca     1380

gcccucgccg gggcacucga agccgaaaug gacggcgcca agggacgacu aagcuccggc     1440

caccucaaau gccgacuaaa aauggacaag cuacggcuca aaggcgucuc guacagccuc     1500

ugcacagcgg ccuucaccuu caccaaaauc cccgccgaaa cucuacacgg cacagucacc     1560

guagaagucc aguacgcagg aaccgacggc cccugcaagg ugccagcaca aauggccgua     1620

gauaugcaaa cccuuacccc cguaggccgc cucauuaccg ccaaccccgu gauaaccgag     1680

agcaccgaaa acagcaaaau gaugcuagaa cucgaucccc cauucggcga caguuacauc     1740

gucaucggcg ucggagagaa gaaaaucaca caccacuggc acagauccgg uucaacaaua     1800

ggaaaagccu ucgaagcaac cguccgcgga gccaaacgca uggcaguacu cggcgacacc     1860

gcaugggacu ucggauccgu cggaggagcc cuaaacuccc ucggcaaggg aauacaccaa     1920

aucuucggcg ccgccuucaa aagccucuuc gguggaaugu caugguucag ccaaauccug     1980

aucgggacac uucuaaugug gcuuggccua aacaccaaaa acggcagcau cagucucaug     2040

ugucuagccc ucggcggagu acucauauuc cuaucaacag caguauccgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  5024
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5024
gggagaaagc uuaccauggg ggcggauacc uccguaggaa uagucggacu acuacucacu       60

accgcaaugg ccgcagaggu aacacgacgc ggcagcgccu acuacaugua ccuagaccga      120

aacgacgcag gagaagcaau cagcuucccc acgacacugg gaaugaacaa gugcuacaua      180

cagaucaugg accucggcca caugugcgac gcaaccauga gcuacgaaug cccaaugcua      240

gacgaaggag uagaacccga cgacguagac uguuggugua acaccacaag caccuggguc      300

gucuacggaa caugccacca caagaaggga gaggcccgcc gcagcagacg agccguaaca      360

cuacccucgc acagcaccag aaaacuacag accagaagcc aaacauggcu agaaagcaga      420

gaauacacaa aacaccuaau ccggguagaa aacuggauau uuaggaaccc gggauucgca      480

cuagcagccg cagcaauagc auggcuacua gggagcucaa ccucccaaaa aguaauauac      540

cuagucauga uccuacucau cgcgccagca uacuccaucc ggugcauagg aguaucaaac      600

agagauuucg uagaaggcau gucagggggc accuggguag acauaguccu cgaacacgga      660

gggugcguaa cggugauggc acaagauaaa ccaaccguag acaucgagcu aguaacaaca      720

acagucagca acauggccga aguaaggucg uacugcuacg aagccaguau cucagacaug      780

gccagcgacu cacgaugccc aacccaagga gaagccuacc uagacaaaca guccgacaca      840

caguacguau gcaaacgcac gcucgucgac cgaggcuggg gaaacggcug cggccuguuc      900

ggaaaaggga gccuggucac augcgcaaaa uucgccugcu ccaagaaaau gaccggaaaa      960

uccauacaac ccgaaaaccu agaauaccgg aucaugcuca guguccacgg cagucagcau     1020

agcggcauga ucguaaacga caccggacac gaaacagaug aaaaccgagc aaaagucgaa     1080

auaaccccaa acucgccccg agcagaagcc acauuaggcg gcuucggauc ccuaggacua     1140

gacugcgaac cccgaaccgg acucgacuuc ucagaccugu auuaucucac gaugaacaac     1200

aaacacuggc ugguacacaa ggaaugguuc cacgacauac cacuaccaug gcacgcugga     1260

gcugacaccg gaacaccaca cuggaacaac aaagaggccc uaguagaguu caaagaugca     1320

caugccaaac gccaaaccgu gguaguccua gggucccaag aaggagccgu gcacaccgca     1380

cucgccggcg cacuggaagc agaaauggac ggagccaaag ggcgacuaag cuccggccac     1440

cuaaaaugca gacucaaaau ggacaaauua cgacuuaaag gcguaagcua uucacugugc     1500

acggcggcau ucacauucac caaaauaccc gccgaaaccc uacacggaac agucaccgua     1560

gaaguccaau acgccgggac agacggaccg ugcaaagucc cagcccaaau ggccguagac     1620

augcaaacac ucacccccgu aggaagacuc auaaccgcaa acccggucau caccgaauca     1680

accgaaaaca gcaaaaugau gcucgaacua gacccgcccu ucggggacag cuauauagug     1740

auaggcguag gagaaaagaa aauaacgcac cacuggcacc gaagcggcag cacaauaggg     1800

aaagccuucg aagccacagu acgaggagcg aaaagaaugg caguccuggg agacaccgcg     1860

ugggacuucg gaucgguagg aggcgcacua aacagccugg gaaaaggaau acaccaaauc     1920

uucggcgccg ccuucaaauc acucuucggc ggaaugucau gguucuccca gauacuaauc     1980

gggacccucc ugauguggcu aggucuaaac accaaaaaug gcucaaucuc acuaaugugc     2040

cuagcgcuag gaggcguccu caucuuccug uccacagccg uaucggccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  5025
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5025
gggagaaagc uuaccauggc agacaccagu guaggcaucg uaggacuccu acuaaccaca       60

gcaauggccg cugaagucac acgacgcgga agcgcauacu acauguaccu agaccguaac      120

gacgccggcg aagcaaucuc guuccccacg acccucggaa ugaacaaaug cuacauucaa      180

auuauggacc uagggcacau gugcgacgcg acaaugagcu acgaaugccc caugcucgac      240

gaaggcgugg aaccagacga cgucgacugc uggugcaaca caacaagcac augggugguc      300

uauggaaccu gccaucacaa gaaaggcgaa gcacgaagaa gccgccgggc aguaacacuu      360

ccgagucaca gcacccgaaa acuccaaaca cgcagccaaa cauggcuaga aagccgcgaa      420

uauacgaaac accuaaucag aguagaaaac uggauauucc gaaacccugg cuuugcacuc      480

gccgcagcag ccauagcaug gcuccugggc agcagcacaa gccaaaaagu caucuaccua      540

gucaugauac uccuuaucgc ccccgcauac ucaauccgcu gcaucggcgu cucgaacagg      600

gacuucgugg agggcaugag ugggggaacc uggguagaca ucguccuaga acacggcggc      660

ugcgucacgg uaauggcaca ggacaaacca acaguagaua uagagcuagu aaccacuaca      720

guauccaaca uggcagaagu ccgcagcuac ugcuacgaag caucgauauc ggacauggcc      780

agcgauucca gaugcccaac ccaaggggag gccuaccucg acaaacaaag cgacacccag      840

uacguaugca aaagaacacu cguggacaga ggguggggca acggcugcgg acuauucggc      900

aagggaucac ugguaaccug cgccaaauuc gccugcucca agaaaaugac cggcaaaagc      960

auacagccag aaaaccuaga auacagaauc augcucuccg uacacggcuc gcaacacagc     1020

ggaaugaucg uaaacgacac aggacacgaa acagacgaaa accgcgcaaa agucgaaauc     1080

accccgaacu ccccgcgcgc agaagcaaca cuaggcggcu ucggaagccu cggacuagac     1140

ugcgaaccuc gaaccggccu cgacuucagu gaccucuacu accucacaau gaacaacaaa     1200

cacuggcuag uacacaaaga augguuccac gacauccccc uacccuggca cgcgggcgca     1260

gacacaggca caccacacug gaacaacaaa gaagcccugg uggaguucaa ggacgcccac     1320

gcaaaacgac aaaccgucgu cguacuuggg agucaagaag gcgcaguaca cacagcacuc     1380

gccggagcac ucgaagccga aauggacggu gccaaaggac gccugagcag cggccaccuc     1440

aagugcagac uaaagaugga caaacuaaga cucaaaggag ugagcuacag ccuuugcacc     1500

gcagccuuca ccuucacaaa aauaccggcc gaaacccugc acggaacggu caccguagaa     1560

guccaguacg ccggaacuga cggacccugc aaggucccag cacaaauggc gguagacaug     1620

caaacccuaa caccaguagg gagauuaaua accgccaacc ccguaaucac agaaagcacg     1680

gaaaacagca agaugaugcu agaacucgac ccgcccuucg gggacagcua cauaguaaua     1740

ggagucggag aaaagaagau cacccaccac uggcaccgaa gcgggagcac caucgguaaa     1800

gcguucgaag caacaguacg cggcgccaaa cgcauggccg uacucgguga caccgccugg     1860

gacuucggcu cggucggcgg ggcccucaau agccuuggca aaggcauuca ccaaaucuuc     1920

ggcgccgcau ucaaaagccu auucggcgga augagcuggu ucagccagau acucauaggu     1980

acacuucuaa uguggcucgg gcucaacacc aaaaacggcu caaucagccu aaugugccug     2040

gcccucggag gagugcuaau cuuccuaagc acagccguca gcgccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  5026
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5026
gggagaaagc uuaccaugga cacaagcguc ggcaucguag gacuacuacu cacaacagcc       60

auggcagcug aagucaccag acgcggcucc gcauacuaca uguacuuaga ccgcaacgac      120

gccggcgaag ccaucucauu cccaacaaca cugggaauga acaaaugcua cauccaaauc      180

auggaccucg gacacaugug cgacgcaacg augucauaug agugcccuau gcuggacgaa      240

ggagucgaac cagacgacgu agacugcugg ugcaacacca cgagcacgug ggucguauac      300

ggcacuugcc accacaagaa aggggaagcc cgccgcucac gccgagccgu aacccuacca      360

ucccacucaa cccggaaacu acaaacacgc ucccaaacau ggcuagaaag cagagaguac      420

acaaaacacc ugauacgcgu cgaaaacugg auauuccgaa accccggauu cgcccucgcc      480

gcugccgcca uagccuggcu ccugggcagc uccaccucac agaaagucau cuaucucgua      540

augauccuac ucaucgcccc cgcauacagc auacgaugca uaggcgucuc aaaccgcgac      600

uucgucgaag gaauguccgg aggcaccugg guugauaucg uccuagaaca cgguggaugc      660

guaaccguca uggcacaaga uaaacccacc gucgacaucg aacucguaac aaccacaguc      720

ucgaacaugg ccgaaguacg auccuacugc uacgaagcca gcaucucgga cauggccucc      780

gacagccgcu gccccaccca aggcgaagca uaccucgaca aacaaagcga cacucaauac      840

guaugcaagc ggacacuugu agaccgaggc ugggggaacg gcugcggacu cuucggcaaa      900

ggcagccuug uaacgugcgc aaaguucgcc ugcucaaaga aaaugaccgg aaaauccaua      960

caacccgaaa accuugaaua ccgaauaaug cuaagcgucc acggaucgca acauuccgga     1020

augaucguca acgauaccgg ccacgaaacc gacgaaaacc gagcaaaagu cgaaaucaca     1080

cccaacagcc cccgcgcaga agccacccua gggggcuucg gaagccucgg acuagacugc     1140

gaaccgcgca caggccucga cuucagcgac cuguacuacc ucaccaugaa caauaaacac     1200

uggcuagucc auaaagagug guuccacgau auccccuuac cauggcacgc aggagcagac     1260

accggcaccc cacacuggaa caacaaagag gcacucgucg aguucaaaga cgcacacgcc     1320

aaacgccaaa ccgucgucgu acucggcuca caagaaggcg ccguccauac agcccuggcg     1380

ggagcccuag aagcagaaau ggacggagcc aaaggcagac uaagcucggg ccaccucaaa     1440

ugccgccuaa aaauggacaa acucagacuc aaaggaguaa gcuacucuuu augcaccgcc     1500

gcguucacau ucacaaaaau ucccgcagaa acccuccacg gaacaguaac cguagaagua     1560

caauacgccg gcaccgacgg ccccugcaaa gucccggccc agauggccgu ugacaugcaa     1620

acccuaacac ccgucggcag acuaauaacc gccaacccag uaaucaccga auccacggaa     1680

aacucaaaaa ugauguuaga gcuagaucca ccguucggag acagcuacau cgugaucggc     1740

gucggcgaaa agaaaauaac ccaccacugg caccgcagcg gaagcaccau aggcaaagcc     1800

uucgaagcca ccguccgagg ggcgaaacgc auggcggucc uaggagacac agcuugggac     1860

uucggcagcg uaggcggagc gcuaaacuca cucggcaaag gcauccacca aaucuucggc     1920

gcggcauuca aaucguuguu cggaggaaug ucaugguucu cacagauacu caucggcaca     1980

cuccugaugu ggcuaggccu caacaccaaa aauggcucaa uaagccuaau gugccuagca     2040

cucggcggcg uccuaaucuu ccugagcaca gccgucagcg cugacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  5027
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5027
gggagaaagc uuaccaugac aagcguagga auugucggac uccuacuaac aaccgcgaug       60

gcagcagaag uaacgcgacg cggcucagcc uacuacaugu accuagaccg aaacgacgcc      120

ggagaagcca ucucauuccc gaccacacuc gggaugaaua aauguuacau acaaauuaug      180

gaccuaggcc acaugugcga cgcaaccaug ucguacgaau gcccgaugcu cgacgaggga      240

gucgaaccgg acgacguaga cugcuggugc aacaccacgu ccacaugggu cgucuacggg      300

accugccacc acaagaaagg agaagcacga cgaagccgac gggcgguuac ccuaccaagc      360

cacagcacac gcaaacucca aacacgcagu caaaccuggc ucgaaucccg agaauacacc      420

aaacaccuaa uacgcgucga gaacuggauc uucaggaauc caggauucgc ccucgcagca      480

gcagccaucg cauggcuccu gggcucgagc acuagccaaa aaguaauaua cuuaguaaug      540

auccuacuga ucgcccccgc auacucaaua cgaugcaucg gcguaagcaa ccgcgacuuc      600

gucgaaggca ugucaggagg aacaugggua gacauagucc ucgaacacgg gggcugcguc      660

acgguaaugg cacaggacaa accaacaguc gacauagagc uagugaccac cacagucagu      720

aacauggcgg aaguccgauc guacugcuac gaagccagca uaucagacau ggccucagac      780

ucacgcugcc ccacacaggg agaggccuac cucgacaagc aaagcgacac acaauauguc      840

ugcaaaagaa cauuagucga ucgcgggugg ggcaacgggu gcggccucuu cggcaaaggc      900

agucucguaa caugcgcaaa auuugcaugc uccaagaaaa ugacaggaaa auccauacaa      960

cccgaaaauc ucgaguacag aaucaugcua uccguccacg gcucccaaca uagcgggaug     1020

auagugaacg acacgggcca cgaaacagac gaaaaccgag ccaaagucga aauaacaccc     1080

aacagucccc gcgcagaagc aacacuaggc ggguucggca gccuaggcuu agacugcgaa     1140

ccacgcacag gcuuagacuu cagcgaccua uacuaccuca ccaugaacaa caaacacugg     1200

cugguccaca aagagugguu ccacgacauc ccacuccccu ggcaugcagg agcugacacg     1260

ggcaccccac acuggaacaa caaagaggcu cucguagagu ucaaagacgc acacgcaaaa     1320

cgacaaacag ucgugguccu aggcucacaa gaaggagcgg uacauaccgc acuagccgga     1380

gcccucgagg ccgaaaugga cggagcgaag gggcgccuaa gcucaggaca ccuaaagugc     1440

cgacuuaaaa uggacaaacu acgauugaaa ggagucuccu acucccucug cacagcagcc     1500

uuuacauuua ccaaaauacc ggccgagacc cugcacggga cuguaacagu ugagguacaa     1560

uacgccggga ccgacggccc augcaaagua ccagcccaaa uggcaguaga caugcagacc     1620

cuaacccccg uagggcgccu gauaaccgcc aauccaguca ucacagaauc aacggaaaac     1680

ucaaaaauga ugcuagaacu agauccccca uucggcgaca gcuacauagu cauaggaguc     1740

ggagaaaaga aaauaaccca ccacuggcac aggucaggcu caacaaucgg aaaagcauuc     1800

gaagccacgg uacgaggcgc gaaaagaaug gcgguccucg gcgacacugc augggacuuc     1860

ggauccguag gcggcgcccu caacucacuc ggaaaaggaa uccaccaaau cuucggcgcc     1920

gcauucaaau cacuguucgg cgggaugucc ugguucagcc aaauacucau cggaacgcua     1980

cuaauguggc uaggccuaaa caccaagaac gggagcaucu cccugaugug ccucgcccua     2040

ggaggaguac ugauauuccu gagcacagcc guaagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  5028
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5028
gggagaaagc uuaccaugac cuccgucggc aucguaggac uccuccuaac aaccgccaug       60

gcagccgcag uuacacgacg aggaagcgcc uacuacaugu accuagacag aaacgacgcc      120

ggagaagcca uauccuuccc gacgaccuua ggcaugaaca aaugcuacau acaaauaaug      180

gaccuaggac acaugugcga cgcaacgaug ucauaugaau gccccaugcu cgacgaagga      240

guggaaccug acgacguaga cugcuggugc aacacgacau caaccugggu aguauacgga      300

accugccacc acaagaaagg cgaagcccga cguucccggc gagccguaac ccuccccagc      360

cacuccacac gcaaacuaca gacacgauca caaaccuggc uagagucacg agaauacaca      420

aaacaccuga uacgcguaga gaacuggauc uuccgcaacc ccggauucgc acuagccgcc      480

gcugccaucg cauggcuacu aggcagcucg accucacaaa aaguaauaua ccucguaaug      540

auccuccuaa ucgccccagc cuacuccaua cgcugcaucg gggucucaaa ccgcgacuuu      600

gucgaaggca uguccggagg aaccuggguc gacauaguac ucgaacacgg cggcugugua      660

accguaaugg cacaagacaa gccuacagua gacaucgagc uagucacaac aacaguauca      720

aacauggccg aaguacgcuc cuacugcuac gaagcaagca uaagcgacau ggcaagcgac      780

agccggugcc ccacacaagg cgaagccuau cuugacaaac agagcgacac acaauacguc      840

ugcaaaagga cccucguaga cagaggcugg ggcaacggcu gcggacuauu cggaaaaggg      900

ucacucguca caugcgcaaa auucgccugc aguaagaaga ugaccgggaa aagcauccaa      960

cccgaaaacc uagaauaccg uaucaugcuc agcguacacg gcucacaaca cuccggcaug     1020

auugucaaug acaccgguca cgaaacagac gagaaccgcg caaaagugga aauaaccccc     1080

aacucgccca gggccgaagc gacacucgga ggauucggau cccuuggccu agacugcgaa     1140

cccaggacag gacucgacuu cucagaccuc uacuaccuua ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ucacgacauc ccccuaccau ggcacgcggg agccgacaca     1260

ggcacaccac acuggaacaa caaagaagcc cuagucgaau uuaaggacgc acacgcaaaa     1320

agacaaaccg uaguuguccu aggaucacaa gaaggcgcag uacacacggc ccucgccgga     1380

gcccucgaag ccgaaaugga cggcgccaaa ggccgacuaa gcagcggcca ccucaaaugc     1440

cgccugaaaa uggacaaacu ccgacuaaaa ggugucaguu auucccuaug cacagccgcc     1500

uuuaccuuca caaaaauacc cgcagaaacc cuccacggca ccgucaccgu cgagguccaa     1560

uacgccggca cggacggccc cugcaaggua cccgcacaaa uggccgugga caugcaaacc     1620

cugacccccg ugggcagacu cauaacagca aacccaguca uaacagaaag cacagaaaac     1680

uccaaaauga ugcucgaacu agacccgccc uucggggacu cauacauagu aauaggcgua     1740

ggagagaaga aaaucaccca ccacuggcac cgaucgggau cuaccaucgg caaagcauuc     1800

gaagccaccg ugcgaggcgc caaacgaaug gcaguccucg gcgacaccgc augggacuuu     1860

ggcucugucg gaggagcccu aaacagccuc ggcaaaggca uccaccaaau cuucggugca     1920

gcguucaaaa gccucuucgg cggcaugucc ugguucagcc aaauccugau cgggacccua     1980

cucauguggc uaggccucaa uaccaaaaac ggauccauca gccuaaugug ccuggcccua     2040

ggcggagucc ugaucuuccu cucaacagca gucagcgcag acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  5029
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5029
gggagaaagc uuaccaugcg aggcgcggac acgucagucg gcauagucgg ucuacuacuu       60

acaaccgcca uggcagccgc cguuacacgc cggggauccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaagc aaucucguuc ccaaccacac uaggcaugaa caaaugcuac      180

auccaaauua uggaccuagg acacaugugc gacgccacca ugagcuacga gugcccaaug      240

cuagacgaag gaguggagcc agacgacgug gacugcuggu gcaacacuac aucgaccugg      300

gucgucuacg ggaccugcca ccacaagaaa ggagaagcac gaaggucaag acgagcugua      360

acacuaccaa gccacuccac aaggaaauua cagacccgaa gccaaacaug gcucgaaucc      420

agggaauaca ccaaacaucu cauucgcgua gaaaacugga uauuccgcaa cccaggcuuc      480

gcacuagcgg cggccgcgau agcuuggcuc cucgggagcu ccaccucgca aaaaguaauc      540

uaccuaguca ugauccuacu cauagcuccc gcguacagca ucagaugcau cggcguaagc      600

aaccgcgacu ucgucgaagg caugagcggc ggcaccuggg uagacauagu acucgaacac      660

gggggcugug ucaccguaau ggcacaagac aaaccaacgg uggauaucga gcuagucaca      720

acgaccgucu ccaacauggc cgaaguaaga agcuauugcu acgaggcauc cauaagcgac      780

auggcaagcg acucgcgcug cccaacgcaa ggugaagcau accuagacaa acaaagcgac      840

acacaauacg uaugcaaacg cacccucguc gacagagggu ggggcaaugg augcggacuc      900

uucggcaaag gcucccucgu cacaugcgca aaauucgccu gcuccaaaaa gaugacaggc      960

aaaucaaucc aacccgaaaa ccuagaguac cgaauaaugc ucagcgucca cggaagccaa     1020

cacagcggaa ugauagucaa cgacacagga cacgaaaccg acgaaaaccg cgcaaaaguc     1080

gaaaucacac caaacagucc aagagcggaa gcaacacuag gcggauucgg aucacuagga     1140

cuagauugcg agccacgcac aggacuagac uucagcgacc uauacuaccu gacgaugaac     1200

aacaaacacu ggcucguaca caaagagugg uuccacgaca ucccccuccc auggcacgcc     1260

ggcgccgaca ccggcacccc gcauuggaac aacaaagaag cccuaguaga guucaaagac     1320

gcacacgcaa agcgccaaac cgucgucgua cuaggaagcc aagaaggagc gguccacaca     1380

gcccuagccg gcgcccuaga agcugaaaug gacggagcaa aaggaagacu aagcagcggu     1440

caccucaaau gcagacucaa aauggacaaa cuacgccuca aaggcguauc guacucccuc     1500

ugcaccgccg ccuucacauu caccaaaaua ccagcagaga cacuacacgg aaccgugaca     1560

guggaagucc aauacgccgg caccgaugga ccaugcaaag uacccgcaca aauggcagua     1620

gacaugcaaa cccugacacc cgucggcaga cuaaucacag ccaacccagu cauaaccgaa     1680

agcaccgaga acagcaagau gaugcucgag cucgacccgc cauucgggga cagcuacauc     1740

gucauaggag ugggagaaaa gaaaaucacc caccacuggc accgaagcgg cucaacaaua     1800

ggaaaagcgu ucgaagcgac cguccgagga gccaaacgca uggcaguacu cggugacaca     1860

gccugggacu ucggcucagu aggcggcgcc cuaaacucuc uaggaaaagg cauacaccaa     1920

aucuuuggcg cagccuucaa aucacucuuc ggcggcauga gcugguucuc ccagauccua     1980

auaggcacac uacuaaugug gcucggacuc aacaccaaaa auggcucgau cagccugaug     2040

ugccucgcac uaggcggcgu acugaucuuc cuauccacag cgguaagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  5030
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5030
gggagaaagc uuaccaugcg cggccuaucg ucacggaaaa gacggagcca cgacgugcua       60

acagugcaau uccucauacu cggcaugcua cuaaugaccg gaggcgucac ccgccgaggc      120

ucagccuacu acauguaccu agacaggaac gacgccgggg aagccauaag uuuccccacg      180

acacugggca ugaacaaaug cuauauacaa aucauggauc uaggccacau gugcgacgcc      240

accaugagcu acgaaugccc gaugcuagac gaaggagucg agccagacga cguggacugu      300

uggugcaaca caacauccac auggguaguc uacggaacau gccaccauaa gaaaggagaa      360

gcccgacgcu caaggagagc cgugacccuc ccaagccacu cgaccagaaa acuccaaaca      420

agaagccaaa cguggcucga aucgagggaa uacacgaagc accuaauaag agucgaaaac      480

uggauauucc gaaacccagg auucgcacug gccgcggccg ccaucgccug gcuacucgga      540

agcagcacga gccaaaaagu aauuuaucuu guaaugaucu ugcugauagc cccggccuac      600

agcauccgau gcaucggcgu uucgaauaga gacuucguag aaggcaugag cggcgguacc      660

uggguagaua ucguccucga acacggggga ugcgugaccg uaauggccca ggacaaaccg      720

accgucgaca uagagcuagu aaccacaaca gucagcaaca uggcagaagu caggaguuac      780

ugcuaugaag caucaauauc agacauggca agcgacagca gaugcccuac acaaggggag      840

gcauaccucg acaaacaaag ugacacccaa uacgucugua aaagaacccu agucgaccgc      900

ggauggggga auggcugcgg acuauucgga aaagggagcc uagugacaug cgccaaauuc      960

gcaugcucaa agaaaaugac aggaaaaagc auccaaccag aaaaccucga guacagaaua     1020

augcucagcg uccacggguc acagcacagc ggcaugauag uaaacgacac cggacacgaa     1080

accgacgaaa accgagccaa aguagaaaua accccuaaua gccccagagc ugaggcaacc     1140

cuaggaggcu ucggcagcuu gggacucgac ugcgaacccc gaacgggacu cgacuucucc     1200

gaccuauacu accucaccau gaacaacaaa cacuggcucg ugcacaaaga augguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacacaggaa cgccucacug gaacaacaag     1320

gaagcccuug uagaguucaa agacgcgcac gcaaaacgcc aaacgguggu cgugcucgga     1380

agucaagaag gagccgugca cacggcacua gccggcgcgc ucgaagccga aauggacgga     1440

gcaaaggggc gacugagcag cggacaccug aaaugccggc ugaaaaugga caaacugaga     1500

cuaaaaggag ucagcuacag ccuaugcacc gccgcauuca cauucacaaa aauaccggcc     1560

gaaacacuac acgguacagu cacgguagaa guccaauacg cggggacaga cggacccugc     1620

aaagucccag cgcaaauggc gguagauaug cagacacuca cacccguggg uagauuaaua     1680

acagcaaacc ccgucaucac agaaagcacg gaaaacagca aaaugaugcu agaacuagac     1740

ccacccuucg gagauucaua cauagucaua ggcguaggcg aaaagaaaau aacccaccac     1800

uggcaccgca gcggcagcac aaucggaaaa gcauucgaag caacggugag gggagccaaa     1860

cgaauggcgg uccucggaga uacagccugg gacuucggaa gugucggggg ugcacugaac     1920

agccuaggca aaggcauaca ccaaaucuuc ggagcagcgu ucaaaagccu cuucggggga     1980

augagcuggu ucucccaaau auugauagga acucuacuca uguggcucgg gcuaaacacu     2040

aaaaauggca gcaucagccu caugugccug gcccucggag gcguccucau auuccuaagc     2100

acagccguca gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  5031
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5031
gggagaaagc uuaccaugcg aggccuuagc ucccgcaaac gacgcagcca cgacguacua       60

accguccaau uccuaauacu aggcaugcuc cucaugacag gcgccguaac aagacggggu      120

uccgcauacu acauguaccu cgacaggaac gacgcgggag aagccauuuc cuucccaacc      180

acacucggca ugaacaaaug cuacauacaa auaauggauc ucggacacau gugcgacgcc      240

accaugucau acgaaugccc aaugcuagac gaaggagugg aacccgacga cguagacugc      300

uggugcaaca ccacauccac cuggguaguc uacggaacgu gccaccacaa gaaaggagaa      360

gcacggcgaa gccgacgagc cguaacccuc cccucccaca gcacucgcaa acuccaaaca      420

cgaucacaaa cauggcuaga aucccgcgaa uacaccaaac accuaauacg cgucgaaaac      480

uggaucuuuc gaaacccagg cuucgcccuc gcagcagccg cgauagcaug gcuucuugga      540

agcagcacaa gucaaaaagu aaucuacuua guaaugaucc ugcuuauagc gccugcauau      600

ucgauacggu gcaucggggu aagcaaccgc gacuucgucg aaggaauguc aggaggcacc      660

uggguagaca uuguacucga acacggagga ugcguaaccg uaauggccca agacaaaccu      720

acagucgaca uagagcucgu aacgaccaca guaagcaaca uggcagaggu ccgaucauac      780

ugcuacgaag ccucaauaag ugacauggcc ucagacucac gcugcccaac ccaaggcgag      840

gccuaccuag acaagcaauc cgacacacag uacgucugca aacgcacacu cgucgacaga      900

ggguggggca acgguugcgg ccuuuucggc aaaggaucac ucguaacaug cgcaaaauuc      960

gccugcucca agaaaaugac uggaaaaagc auacaaccag aaaaccuaga guacagaaua     1020

augcuauccg uacacgggag ccagcacuca ggaaugaucg ucaacgacac gggacacgaa     1080

accgacgaaa accgcgcaaa aguagaaaua acacccaacu caccccgagc agaagcgacc     1140

cuaggaggau ucggaucccu cggacuagac ugugaaccca gaacaggccu cgacuucagc     1200

gaccucuacu accucaccau gaacaacaaa cacuggcuug uccacaaaga gugguuccac     1260

gacauccccc uacccuggca cgccggagca gacaccggca ccccacacug gaacaacaaa     1320

gaagcccucg ucgaguucaa agacgcgcac gcaaaacgac aaaccgucgu cguucuugga     1380

ucccaggaag gagccgucca uacagcacua gcaggcgccc uggaagccga aauggacggc     1440

gccaaggggc ggcuaucauc aggccaccua aaaugucgcc uaaagaugga caaacuccga     1500

cuaaaagggg ucucauacuc ucuaugcacc gcggcauuca ccuucaccaa gauccccgca     1560

gaaacccuac acggaaccgu cacaguagaa guacaauacg ccggcacaga cggccccugc     1620

aaggucccag cccaaauggc cguagacaug caaacacuga ccccaguagg acgacuaaua     1680

accgcgaacc ccguaauaac cgaaucgacc gaaaacucaa aaaugaugcu cgaacuagac     1740

ccaccguuug gcgacagcua caucgucauc ggcgucggag agaaaaagau aacccaccac     1800

uggcacaggu ccggaagcac caucgguaag gcuuucgaag caaccguccg cggagccaaa     1860

cgcauggccg uccuaggaga cacagcaugg gauuucggca gcgucggcgg agcccugaac     1920

agccucggaa aaggaauaca ccaaauauuc ggagccgcgu ucaaaagccu auucgggggc     1980

auguccuggu ucucacaaau ccuaaucggc acacuacuga uguggcucgg ccucaacacc     2040

aaaaacggga gcauuagccu caugugucua gcucuaggcg gcguacuaau cuuccuaucc     2100

acagcaguga gcgcggacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  5032
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5032
gggagaaagc uuaccaugcg caucauaaac gccagaaaag aaaagaaacg aaggggagcc       60

gacacaucag uaggcaucgu cggacuacuc uugaccaccg caauggccgc ggaagucacc      120

agaaggggcu cagcauacua cauguaccua gaccguaacg acgccggaga agcaaucucc      180

uuccccacca cccuuggaau gaacaaaugc uacauacaaa uaauggaucu aggccauaug      240

ugcgacgcca ccauguccua cgaaugcccc augcucgacg aaggaguaga gcccgacgac      300

guggacuguu gguguaauac caccagcaca ugggucgucu augggaccug ccaccacaag      360

aaaggcgaag cacgaagaag ucgacgagca guaacacuac cgagccacag cacccgaaaa      420

cuacaaacaa gaagucaaac auggcuagaa agccgagaau acacgaaaca ccuaauaaga      480

guagagaacu ggauauucag aaacccaggg uucgcccucg ccgccgcggc caucgcaugg      540

cugcuaggcu ccagcacauc gcaaaaagua auauaccuag ucaugauucu acuaaucgca      600

cccgcauaca gcauccgcug cauaggcguc ucgaaccgcg acuucgucga gggcauguca      660

ggaggcacau ggguggacau cgucuuagaa cacgguggau gcgucaccgu cauggcccaa      720

gacaaaccca ccguagacau cgagcuggua accaccaccg uauccaacau ggccgaagug      780

cgguccuacu gcuacgaagc cucaauaagc gacauggccu ccgacagcag augcccaacc      840

caaggggagg cguaucucga caaacaaagc gacacccaau acgucugcaa acgaacacuc      900

guagaccgcg gguggggaaa cggcugcgga cucuucggaa aaggcagccu cguaaccugu      960

gccaaauucg caugcucgaa gaaaaugacc ggaaaaucca uacaaccaga gaacuuagaa     1020

uaccgcauca ugcuaagcgu acauggcagc caacacagcg gcaugauagu aaacgacaca     1080

ggccaugaaa cagacgaaaa ccgagcaaaa gucgaaauca cccccaacag cccgagagca     1140

gaagccacac uaggaggauu cggcagccua ggccuggacu gcgaaccaag gacaggccuc     1200

gacuucagcg accucuacua ccucaccaug aacaacaagc acuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu ccccuggcac gcaggcgccg acacagggac accccauugg     1320

aauaacaaag aagcacuggu agaguucaaa gacgcccacg ccaagagaca aaccguagua     1380

guacuaggcu cccaggaagg ggcaguccac acagcacuag cgggagcacu ggaagcagaa     1440

auggacggcg ccaaagggcg acuaagcagc ggccaccuga aaugcagacu uaaaauggau     1500

aaacuacgac uaaagggcgu cagcuacagc cucugcaccg cugccuucac auucacgaaa     1560

auccccgccg aaaccuuaca cggcacgguc acagucgaag uacaauacgc cggaaccgac     1620

gguccaugca aaguacccgc acaaauggcc gucgacaugc agacacuaac acccgucgga     1680

cggcuaauaa cagccaaccc cguuaucaca gaaucgaccg aaaauucaaa aaugaugcuc     1740

gaacuagacc caccguuugg agacuccuac aucguaaucg gcgucggaga aaagaaaauc     1800

acacaccacu ggcaccgcag cggcuccacg auagggaaag cauucgaggc caccguccga     1860

ggagcaaaaa gaauggccgu ccucggcgac acagcauggg acuucggcag cgucggaggc     1920

gcgcucaaca gucucggaaa aggcauccac caaauauucg gcgcagcauu caaaagccuu     1980

uucggcggca ugagcugguu uucacaaauc cuaauaggaa cccuccucau guggcucggc     2040

cuaaacacca aaaacggguc aauaagccua augugccugg cccucggcgg gguccuaaua     2100

uuccucucca cggcagucag cgcaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  5033
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5033
gggagaaagc uuaccaugau aaacgcccga aaagagaaaa agcgccgagg agccgacaca       60

uccguaggua uagucggccu cuuauuaaca acagcaaugg cugccgaagu aacaagacgc      120

ggcagcgcau auuacaugua ccuugaccga aacgaugcag gggaagcaau aucauuucca      180

acaacacuug gcaugaacaa augcuauauc caaaucaugg acuugggaca caugugcgac      240

gccacaaugu cauacgaaug cccgaugcua gacgaagggg uggaaccgga cgacguugac      300

ugcuggugca acacaacuuc aaccugggua gucuacggaa ccugccacca caagaaaggc      360

gaagcaagaa gauccagacg ggccgucaca cuccccagcc acagcacuag aaaacuacaa      420

acccgaagcc aaacauggcu cgaaagccgc gaauacacaa agcaucugau ccgaguggaa      480

aacuggaucu uccgaaaccc cggcuucgcc cucgccgccg cagccauagc auggcuccug      540

ggauccucca cgucacaaaa agugauauac cuaguaauga uccugcucau agccccggcc      600

uacuccauac gcugcauagg cguaagcaac cgagacuucg uagagggaau gucagggggc      660

accugggucg acaucguccu agaacacggg ggcugcguca cagucauggc gcaagacaag      720

cccacagucg acaucgagcu cgucacaaca accguaucaa acauggcaga gguaaggucc      780

uacugcuacg aagcgucaau cucagacaug gccagcgacu cccguugccc cacacaaggc      840

gaggccuacc uagacaaaca auccgacaca caauacguau gcaaacgaac acucguagac      900

cggggcuggg gaaacggcug cggacucuuc ggcaaaggca gccucgucac augcgcgaaa      960

uucgccugcu caaagaaaau gaccggaaaa agcauccaac ccgagaaccu agaauaccga     1020

auaaugcuaa gcguacacgg gucccaacac uccggaauga ucgucaacga caccggacac     1080

gaaaccgacg aaaacagagc uaaaguagaa aucacgccaa acucgccacg cgcagaagcc     1140

acgcucggag gauucggauc acuagggcua gacugcgagc cacgcaccgg gcuagacuuc     1200

uccgaccuau acuaccuaac caugaacaac aaacacuggc uuguucacaa agaaugguuc     1260

caugacaucc cccuccccug gcacgcagga gccgacacag gaaccccaca cuggaacaac     1320

aaagaagccc uaguagaguu caaagacgca caugcaaaac gccagaccgu cgucguacua     1380

ggaucccaag aaggagcugu ccauaccgca cucgccggcg cccucgaagc cgaaauggac     1440

ggagccaaag ggcgccuaag cuccggacac cucaaaugcc gacuaaaaau ggacaaacua     1500

agacugaaag gcgucagcua cagccuaugc acagccgcau ucacauucac caaaauucca     1560

gcagaaacgc uccacggaac agucaccgua gaaguacaau acgccggcac agacggcccc     1620

ugcaaagugc cagcccaaau ggcgguagac augcaaacac ucacaccagu cggccgccua     1680

auaacggcua acccaguaau caccgaaagc accgaaaaua gcaaaaugau gcuugaacuc     1740

gacccgcccu ucggagacag cuacaucguc aucggcgucg gggaaaagaa aauaacacac     1800

cacuggcacc gaagcggguc cacaaucggc aaagccuucg aagcaaccgu aaggggcgca     1860

aaacgcaugg ccguacucgg agacacggcc ugggacuucg guuccgucgg aggcgcacua     1920

aacagccugg gcaaaggcau ccaucaaauc uucggagcag cauucaaauc acuuuuuggg     1980

ggaaugagcu gguucuccca aauccugauc ggcaccuuac uaauguggcu gggccuuaac     2040

accaaaaaug gcagcaucuc gcucaugugc cucgcacucg gaggaguccu aaucuuccug     2100

agcacagccg uauccgccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  5034
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5034
gggagaaagc uuaccaugau aaucaacgca agaaaggaaa agaaacgccg cggugccgac       60

acaucaguag gcauagucgg ccuccuccuc acgacagcua uggccgcaga aguaacacga      120

cgcggaagcg cguacuacau guaccucgac cgcaacgacg ccggagaagc gaucuccuuc      180

ccgaccacac ucggcaugaa caaaugcuac auccaaauca uggaucucgg acacaugugc      240

gacgccacca uguccuacga gugcccaaug cuagacgaag gagucgaacc cgacgacgua      300

gauugcuggu gcaacacgac cuccacaugg gucguuuacg gaaccuguca ccacaagaag      360

ggcgaagccc gacgaagccg cagagcaguc acccuaccca gccacuccac ucgcaaacuc      420

cagacacgaa gccaaacaug gcucgagagc cgagaguaca caaaacaccu cauaagggua      480

gaaaacugga ucuuccgaaa uccaggcuuc gcccuagccg cagccgccau cgcguggcuu      540

cuaggcucaa gcacgucaca aaaggucauc uaccucguca ugauccuacu aaucgcucca      600

gccuauucaa uccggugcau agguguaagc aaccgagacu ucguagaagg caugucagga      660

ggaacauggg uagacaucgu ccuggaacau ggcggaugcg uaaccgucau ggcccaagac      720

aaacccacag uagacaucga acucgugacc accaccguaa gcaacauggc agaaguccga      780

agcuacugcu acgaggcuuc aaucagcgac auggcaucag acagcagaug ccccacacaa      840

ggcgaagccu accuagacaa gcagucagac acccaauaug uaugcaaacg cacccuaguc      900

gauaggggau ggggaaacgg augcggacuc uucgguaaag gcagucucgu gaccugcgcg      960

aaauucgcgu gcuccaagaa aaugacgggc aaaagcauuc aacccgaaaa ccuagaauac     1020

agaaucaugc uaucagucca cggcucccaa cacuccggca ugauugucaa cgacaccggc     1080

cacgaaaccg acgaaaaccg agcaaaaguc gaaaucacac ccaacagccc gcgcgccgaa     1140

gccacacucg gcggcuucgg cagccugggc cucgacugug aaccccgcac aggacuagac     1200

uucagcgacc ucuacuaccu gacaaugaac aacaaacacu ggcucgucca caaagaaugg     1260

uuccacgaca uuccccuccc cuggcacgcc ggcgcagaua ccggaacccc acacuggaac     1320

aauaaagaag cacucguaga guucaaggac gcgcacgcaa aacggcaaac agucgugguc     1380

cugggcucgc aagaaggcgc aguccacaca gcccucgccg gcgcccuaga agccgaaaug     1440

gacggagcaa aagguagacu cagcucagga caccuaaaau gcagacucaa gauggacaaa     1500

uuacgccuca aaggcguauc uuacucccua ugcaccgccg cauucaccuu uaccaaaauc     1560

ccggcagaaa cgcuccaugg aacagucacc gucgaggucc aauacgccgg aaccgaugga     1620

ccuugcaaag uccccgccca aauggcgguu gacaugcaga cccugacccc cgucggccga     1680

cucaucaccg ccaacccagu caucacugaa agcacagaaa acuccaaaau gaugcucgaa     1740

cucgacccac ccuucggcga caguuacauu gucaucggcg ucggggaaaa gaagaucaca     1800

caccauuggc accgguccgg aagcacgauu ggaaaggccu ucgaagcaac cgucagagga     1860

gccaaaagaa uggcgguacu cggcgacacc gcaugggacu uuggcuccgu cggcggagca     1920

cuuaacucac ucggaaaagg cauacaccaa auauucggug cugccuucaa aucacucuuc     1980

ggcggcauga gcugguucuc acagauacua aucggaacac uacuaaugug gcuaggccuc     2040

aacaccaaaa acggaagcau aucacuuaug ugccuagccc ucggcggagu acuaaucuuc     2100

uuguccacag cagucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  5035
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5035
gggagaaagc uuaccaugaa cgccagaaaa gaaaagaaac gaagaggcgc cgacaccagc       60

guuggaaucg ucggacuacu acuaaccacc gcgauggcag ccgaagucac gagaagggga      120

agcgccuacu acauguaccu agacagaaac gacgcaggcg aggcaauaag uuuccccaca      180

acgcuaggaa ugaauaaaug uuacauacaa auaauggauc ucggacacau gugcgacgca      240

acaaugagcu acgaaugccc aaugcuagac gaaggagucg aaccagacga uguggacugc      300

uggugcaaca caacuagcac augggucguc uacggcacau gccaccacaa gaaaggagaa      360

gcacgaagga gucgcagagc cgucacgcua cccagccacu caacgagaaa acuccagacc      420

aggagccaaa cguggcuaga gagcagagaa uacacaaaac accuaauaag agucgaaaac      480

uggaucuucc gaaacccagg cuucgcccuc gccgccgcag ccauagccug gcuccuaggu      540

ucuaguacca gccaaaaagu aauauaccua gugaugaucc uacugauagc acccgcauac      600

uccauccguu gcauaggagu cagcaaccgc gacuucguag aaggaaugag cggaggaacc      660

ugggucgaua ucguacuaga acacggcggc ugcgucacag ucauggccca agacaaacca      720

accgucgaca uagaacucgu gacaacaaca guuagcaaca uggcggaagu gcgaagcuac      780

ugcuaugaag ccuccauauc agacauggca ucggacagcc gauguccaac acaaggagaa      840

gccuaccuag acaagcaauc cgacacacaa uacguaugca aaagaacccu agucgaucga      900

ggcuggggca acgggugcgg ccuuuucgga aaaggaagcc uggucacaug cgccaaauuc      960

gcgugcagca agaaaaugac aggcaaauca auccaaccag aaaaccucga auaucgaauc     1020

augcucucgg uacacggcag ccaacacucc ggaaugaucg uaaacgacac aggucacgag     1080

acagacgaaa acagagcaaa agucgaaauc acgcccaaca gcccaagagc cgaagcaaca     1140

cucggcgggu ucgggagccu aggacuagac ugcgaacccc gaacaggccu cgacuucucg     1200

gaccucuacu aucuuaccau gaacaacaaa cacuggcuag uccacaaaga augguuccac     1260

gacauccccc uccccuggca cgcaggggca gacaccggaa cgccgcauug gaacaauaaa     1320

gaggcucuag ucgaguucaa ggacgcacac gcgaaaagac aaaccgucgu cguauuaggc     1380

ucacaagagg gcgccgucca cacagcgcuc gcaggggcac uagaagccga gauggacggc     1440

gcgaaaggaa ggcuaucauc aggucaccuc aaaugccggc uuaagaugga caaacugaga     1500

cuaaaagggg uauccuauuc acucugcaca gcagcauuca cauucacaaa aauccccgca     1560

gaaacacugc acggaacagu aaccguagaa guccaauacg caggcaccga cggcccaugc     1620

aaaguaccug cccaaauggc cguagacaug cagacccuaa cgcccgucgg ccggcucauc     1680

acggccaacc cgguaauaac ugaaucaaca gaaaacucua aaaugaugcu ggaacuggac     1740

ccacccuucg gcgacaguua cauagucauc ggugucggug aaaagaaaau cacccaccac     1800

uggcacagaa gcggaagcac uauaggcaaa gcguucgaag ccacaguaag aggcgcaaaa     1860

aggauggcag uacuaggaga caccgccugg gacuucggcu caguaggagg agcacuaaac     1920

ucgcucggca agggaaucca ccaaauauuc ggagccgcau ucaagagccu auucggcgga     1980

augagcuggu ucucacaaau ccuaaucgga acgcugcuaa uguggcucgg ccuaaacaca     2040

aagaacggca gcaucagccu aaugugccuc gcccucgggg gcguccuuau auucuugagc     2100

acagcaguaa gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  5036
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5036
gggagaaagc uuaccauggc caggaaagag aaaaagagaa gaggcgcgga cacaucagua       60

ggaauagucg gacuacugcu aacaaccgcc auggcagcag aaguaacccg aagaggcuca      120

gccuacuaca uguaccucga ccgaaacgac gccggagaag ccauaucauu ccccaccacc      180

cucggaauga acaaaugcua cauccagauc auggaccucg gccacaugug cgaugcaacc      240

augagcuacg aaugcccaau gcucgacgaa ggcguagaac cagacgacgu agacugcugg      300

ugcaacacaa ccaguacgug gguagucuau ggcacaugcc accacaagaa aggcgaagca      360

cgcagaagcc gccgcgcagu cacucuccca ucucacagca ccagaaaacu ccagacaagg      420

agccagaccu ggcuggaauc ccgagaauac accaaacacc uaauaagggu cgaaaacugg      480

auuuuccgca acccaggcuu cgcacucgca gccgcagcca uagccuggcu acugggcagc      540

agcacaagcc aaaaagugau cuaucucguc augauacucc ugauugcccc agcuuacucc      600

aucagaugca ucggcguaag caaccgagac uucgucgagg ggaugucggg aggaaccugg      660

guugacaucg uacucgaaca cggcggaugc guaaccguaa uggcucaaga caaacccacc      720

guugacauag aacuggugac aaccacagua uccaacaugg cagaggugag aucauacugc      780

uacgaggcuu caaucucuga cauggccucg gacagccgcu gccccacaca gggggaagcg      840

uaccucgaca aacaaucaga cacacaguac gucugcaaac gaacccucgu agaccgcgga      900

uggggcaacg ggugcgggcu auucggcaaa ggaucacucg ucacaugcgc aaaauucgcu      960

ugcucgaaga aaaugacagg caaaagcaua caaccagaga accucgaaua cagaauaaug     1020

cugucaguac acggcucaca acacagcgga augauuguca acgacacagg acacgagacc     1080

gacgaaaaca gggccaaagu agaaauaacc cccaacagcc cccgagccga ggcaacacuu     1140

ggaggauucg gcucccuagg auuagacugc gaaccgcgca ccggacugga cuuuagcgac     1200

cuauacuacc ucacaaugaa caacaaacac uggcuagucc acaaagagug guuccacgac     1260

auaccccucc cauggcaugc cggagcagac acaggaaccc cacacuggaa caacaaagaa     1320

gcgcugguag aguucaaaga cgcccacgca aaacgccaaa cagucguggu ccucggcagc     1380

caagaaggcg ccguccacac cgcacucgcc ggcgcacuag aagcagaaau ggacggcgcg     1440

aaaggccggc ucagcuccgg gcaccuaaaa uguagacuaa aaauggauaa gcuacgccua     1500

aaagggguau cauacagccu augcaccgcc gccuucacau ucacaaaaau accggcagaa     1560

acacugcacg gaacaguaac agucgaagua caauacgcag gaaccgaugg cccaugcaaa     1620

guuccagccc aaauggcagu cgacaugcaa acacugaccc cggucggaag acuuaucaca     1680

gcaaaccccg ucaucacaga aucaaccgaa aacagcaaaa ugaugcucga acuagaccca     1740

ccauucggcg acaguuacau agugauaggc guaggagaga agaaaaucac ccaucacugg     1800

cacagauccg ggagcacuau agggaaagcc uucgaggcca caguaagggg agccaaacgc     1860

auggccgucc ucggugacac agcaugggac uucggcucag uaggcggcgc ccucaacucc     1920

cucggaaaag gaauccacca aauauucggg gccgcauuca agagccuguu cgggggcaug     1980

agcugguucu cccaaauccu aauaggcacc cuccucaugu ggcuaggccu caauaccaaa     2040

aacggaagua ucucacucau gugccuagca cuaggcggcg uccucauauu ccucagcaca     2100

gccguuagcg cgugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  5037
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5037
gggagaaagc uuaccaugcg aaaagagaag aaaagacgcg gagcagacac cagcgucgga       60

aucgucggcc uucuccucac uacagccaug gccgccgaag ucacgagacg cggauccgca      120

uacuacaugu aucuagaccg aaacgacgcc ggcgaggcaa ucucauuccc caccacccuc      180

ggcaugaaua aaugcuauau ccagauaaug gaccucggcc acaugugcga cgcaacaaug      240

agcuacgaau gccccaugcu cgacgaggga guagaacccg augacguuga cugcuggugc      300

aacacaaccu cgaccugggu cguguacggc acaugccacc acaagaaagg agaagcgaga      360

cgcucccguc gagcaguaac ccuuccaucc cacagcaccc ggaaacucca aacacgcucc      420

caaaccuggc ucgaaucccg cgaauauaca aaacaucuaa uccgaguaga aaacuggauc      480

uuccgcaacc cgggauucgc ccuggcagcg gccgccaucg ccuggcuacu cgguagcucc      540

accagccaaa aaguaaucua ccucguuaug auacuacuaa ucgccccagc auacucaaua      600

cgcugcaucg gcguaagcaa ccgagacuuc gucgaaggaa ugucaggcgg caccuggguc      660

gacaucgucc uagaacacgg cggaugcguc accgucaugg cgcaagacaa accaaccgua      720

gacauagagc uaguaacaac caccguauca aacauggccg aaguccgaag cuacugcuac      780

gaagcaucga uauccgacau ggccuccgac ucacgcugcc ccacccaagg agaagccuac      840

cuagacaaac agucggauac gcaauacguc ugcaaacgaa cccuagucga ccgaggcugg      900

ggaaacggcu gcggccucuu cggaaaaggg ucccuaguaa ccugcgcaaa auucgcaugc      960

agcaagaaaa ugacaggcaa guccauccag cccgaaaacc ucgaauaucg aauaaugcuc     1020

uccguccacg gcucacaaca cagcggaaug aucgucaacg acaccggaca cgaaaccgac     1080

gaaaaccgag caaaagucga gauaacccca aacucaccuc gcgcugaggc aacacucgga     1140

ggcuucggcu cccucggacu cgacugcgaa ccacgaaccg ggcuagacuu cagcgaucua     1200

uauuaccuca ccaugaacaa caaacacugg cuaguccaca aagaaugguu ccacgacaua     1260

ccacuccccu ggcacgccgg cgcagacacc ggcacgcccc acuggaacaa caaagaagcc     1320

cuggucgagu ucaaagacgc ccacgccaaa cgucaaaccg ucgugguacu cgggucccaa     1380

gaaggagcag uccacacagc acuugcgggc gcccuggaag ccgaaaugga cggcgccaaa     1440

ggacgucucu ccagcggaca ccuaaagugc cgccugaaaa uggacaaacu acgacuaaag     1500

ggagucucau acagcuuaug caccgccgcc uucacauuua ccaaaauacc cgcagaaacg     1560

cuacacggca ccguaaccgu cgaaguacaa uacgcgggaa cagacggacc cugcaaagua     1620

ccugcucaaa uggccguaga caugcaaaca cucacaccag ucggaagacu aaucacagca     1680

aaccccguaa ucaccgaaag caccgaaaau uccaaaauga ugcucgaacu agacccaccg     1740

uuuggagacu cuuacaucgu aauaggagug ggcgaaaaga aaaucacaca ucacuggcac     1800

cgaucgggau cgaccauagg uaaagccuuc gaagcaacag ugcgaggcgc caaacgaaug     1860

gcuguacuug gagacaccgc augggauuuu ggcuccguug gaggagcccu uaacagccuc     1920

ggcaaaggca uacaccagau uuucggcgca gccuucaaau cccucuucgg cggcaugucc     1980

ugguucuccc aaauccucau cggaacccua cucauguggc uaggccucaa cacgaaaaac     2040

ggaucaauau cccucaugug ccuagcacug ggcgguguac uaauauuccu cuccaccgcc     2100

gucucagcgu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  5038
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5038
gggagaaagc uuaccaugaa agagaaaaag aggagaggcg cggacacguc cgucgguaua       60

guaggccuac uucuaacaac cgccauggcc gcagaaguaa cccgucgagg aagcgccuac      120

uacauguacc ucgaccgaaa cgacgccggc gaagcgauca gcuuccccac cacccucggc      180

augaacaaau guuacaucca gauaauggac cucggccaca ugugcgacgc cacgauguca      240

uacgaaugcc ccaugcuuga cgaaggcguu gaaccagacg acgucgacug cuggugcaac      300

acuacaagca ccuggguggu cuacggcacu ugccaccaca aaaagggaga agcacgccgc      360

agccgucgag ccguaacccu ucccucacac agcacccgaa agcuccaaac ccgcucccaa      420

accuggcuag aaucucgaga auacaccaaa caccucauua gagucgaaaa cuggauauuc      480

cgcaauccag gcuucgcccu agcagcagcc gcaaucgccu ggcuucucgg auccucaaca      540

ucacagaaag ucauauaccu cguuaugaua cuccuaaucg ccccagccua cagcaucaga      600

ugcaucggcg ucagcaaccg agacuucgua gaaggcauga gcggaggaac auggguagac      660

aucguccucg aacacggagg augcgucacc guaauggccc aagacaaacc cacaguugac      720

auugagcuag ucacaaccac cgugagcaac auggccgaag ugcgcagcua cugcuacgaa      780

gccuccauau cagacauggc aucagacagc cgcugcccua cacagggaga agcauaccug      840

gacaaacagu ccgacacaca auacgucugc aaacgaaccc ucguagaccg aggcugggga      900

aacggaugcg gacuauucgg caaaggcucc cucgucacau gcgcaaaguu cgccugcagc      960

aagaaaauga ccggcaaauc uauccagccc gagaaccucg aauacagaau aaugcucucc     1020

guacacggau cccaacacag cggcaugauc gucaacgaca caggacacga aacugacgaa     1080

aaucgcgcga aagucgaaau cacacccaac uccccucgag cggaagcuac ccugggcggc     1140

uucggcagcc uaggccucga uugcgaacca cgaacaggcu uagacuucuc cgaccucuac     1200

uaccuaacga ugaacaauaa acacuggcua guccacaaag aaugguucca cgacauaccg     1260

uuaccguggc acgccggggc cgauaccgga acaccacauu ggaacaacaa agaagcacug     1320

guagaguuca aagacgccca cgcaaaacgc caaaccgucg ucguacucgg gucccaagaa     1380

ggcgcagucc acacagcccu cgcaggagcc cucgaagccg aaauggacgg cgcaaaaggc     1440

cgccucagca guggucaccu aaaaugcaga cuaaaaaugg acaaacuccg acuaaaaggc     1500

gucucguacu cccuguguac cgcagcuuuc acuuucacca agauccccgc agaaacccuc     1560

cacggcaccg ucaccguaga agugcaauac gcagggaccg auggccccug uaaaguaccg     1620

gcccaaaugg cagucgacau gcagacccug acacccgugg gccggcucau cacagcaaac     1680

cccguaauaa cagaauccac cgaaaacucc aagaugaugc uagagcuaga ucccccauuc     1740

ggcgacagcu acauagucau cggaguagga gaaaagaaaa ucacacacca cuggcaccgg     1800

uccggcucca ccauaggcaa agcauuugaa gcuacagucc gcggcgccaa acgcauggca     1860

guacuuggcg acaccgccug ggacuucggc uccguaggag gggcgcucaa cagccuaggg     1920

aaaggaaucc accaaaucuu uggagccgca uucaaauccc uauucggcgg cauguccugg     1980

uucucucaaa uccugaucgg cacccuacuc auguggcuag gccucaacac caaaaacggc     2040

uccaucuccc uaaugugccu ggcacucgga ggaguacuca ucuuccuauc cacagccguc     2100

uccgcaugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  5039
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5039
gggagaaagc uuaccaugga gaagaaaaga agaggagcag acaccagcgu aggcaucgua       60

ggccuccuac ucacaaccgc aauggccgcc gaaguaaccc gccggggcuc cgcauacuac      120

auguaccuag accgcaauga ugccggcgaa gcaauaucgu uccccaccac ccucggcaug      180

aacaaaugcu acauacaaau cauggaccua ggacacaugu gcgacgccac cauguccuac      240

gaaugcccca ugcuagacga agggguugaa cccgaugacg ucgacugcug gugcaauaca      300

acgagcaccu gggugguuua cggaacgugc caccacaaga aaggggaggc ccgccgcucc      360

cgcagagccg uaacccuccc cucacacagc accagaaaac uacagacacg aagccagaca      420

uggcucgaau cacgagaaua caccaagcac cucauccgcg uagaaaacug gauauuccgc      480

aaccccggcu ucgcccuagc cgcagccgcc aucgccuggc uccuuggcuc cucaacaagc      540

caaaaaguca uauaccucgu caugauucua cucaucgcgc ccgccuacuc cauccguugc      600

aucggagucu ccaacagaga cuucgucgaa ggcaugucag gaggcaccug gguugacauc      660

guccuugaac acggggguug cguaacggug auggcccagg acaaacccac aguagacaua      720

gaacuuguga ccacaacugu auccaacaug gcggaagucc gcucauacug cuacgaagcc      780

agcaucucgg acauggccuc cgacucccga ugcccaacac aaggcgaagc auaccucgac      840

aaacaauccg acacccaaua cgucugcaaa cggacacucg ucgaccgcgg auggggaaac      900

ggcugugguc uauucggcaa aggaucccua gugaccugcg caaaauuugc uugcucaaag      960

aaaaugacgg gaaaaucaau ccagcccgaa aaccucgaau acagaaucau gcuuuccguc     1020

cacggcagcc aacacagcgg aaugauaguc aacgacaccg gccaugaaac cgacgaaaac     1080

cgggccaaag uagaaaucac cccaaacuca ccccgcgcag aagccacccu cggaggguuc     1140

ggaagccucg gacucgacug cgaaccacgc acuggacuag acuucucaga ccuguacuau     1200

cucacgauga acaauaaaca uuggcuagua cacaaagagu gguuccacga uauaccacua     1260

ccauggcacg ccggagcaga cacaggcaca ccccacugga acaacaaaga agcccucgua     1320

gaguucaaag acgcacacgc caaacgucaa accgucguag uacucgguuc acaagagggc     1380

gcuguacaca ccgcacuagc cggcgcccuc gaagccgaaa uggauggagc caaagggcgc     1440

cuauccucag gccaccuaaa augccgacuc aaaauggaca aacuucgccu caaaggggua     1500

ucauacuccc uaugcacagc cgccuucacc uucacaaaga uacccgccga aacauuacac     1560

ggcaccguca cagucgaggu ccaauacgcc ggcaccgacg gacccugcaa agucccggca     1620

cagauggcag uagacaugca aacccucaca cccgucggcc gccuaauaac agcaaacccc     1680

gucaucaccg aaucaaccga aaacagcaaa augaugcugg aacucgaccc acccuucggc     1740

gacuccuaca ucguaaucgg cguaggcgaa aagaaaauaa cccaucacug gcacaggucc     1800

gguagcacca ucggaaaagc auucgaagca accguccgag gcgccaaacg gauggcugua     1860

cuaggagaca cugccuggga cuucggcucc guuggcggcg cccuaaacuc gcuaggcaaa     1920

ggaauccacc aaaucuucgg cgccgccuuu aaaagccuau ucggaggaau gagcugguuc     1980

ucacaaaucc ucaucggaac acuacuaaug uggcugggac ugaacaccaa aaacggaagc     2040

auaucacuua ugugccucgc acuagguggc guucuuaucu uccucagcac agcgguaucu     2100

gcaugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  5040
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5040
gggagaaagc uuaccaugaa gaaacggcga ggcgccgaca caagcgucgg caucguaggc       60

cuacuccuaa ccaccgcuau ggcagccgaa guaacacgcc gaggaagcgc auacuauaug      120

uaccuagauc gcaacgacgc aggcgaggcc aucagcuucc ccaccacauu aggcaugaau      180

aaaugcuaca uccaaauaau ggaucuaggc cacaugugcg augccacaau gaguuacgaa      240

ugcccaaugc ucgacgaggg agucgaaccc gacgacguag acugcuggug caacaccacu      300

ucaacauggg ucguauacgg aaccugccac cacaagaaag gcgaagcaag gagaucaagg      360

agagcuguca cgcuccccuc acacagcacc cgcaaacuac aaacacgguc ccaaaccugg      420

cuggaaagcc gagaguauac aaaacaccua auccgcgucg aaaauuggau auucagaaac      480

cccggcuucg cgcucgcagc agccgcaaua gcauggcuac ucggcucaag cacuucacaa      540

aaagucaucu aucuagucau gauccuucuc aucgcaccag cauacaguau acgcugcaua      600

gggguaagca accgggacuu cguugaagga auguccgggg gcacgugggu agacauagua      660

cuagaacacg gaggaugcgu aacgguaaug gcccaagaca aacccacagu ggacaucgag      720

cuaguaacga caacagucag caacauggcc gagguacggu cauacugcua cgaggcaagc      780

auaucagaca uggcauccga cucaaggugc cccacacagg gcgaggccua ccucgacaag      840

caaagcgaca cacaauacgu cugcaaacgg acccucguag auagaggcug gggcaacgga      900

ugcggccucu ucggaaaagg gagccuagua acaugcgcaa aauucgccug cagcaagaaa      960

augaccggaa aaagcauaca accagaaaac cuugaauacc gcauaaugcu aagcguacac     1020

ggcagccaac acagcgguau gaucguaaac gacacaggac acgaaaccga cgaaaauaga     1080

gcaaaaguag aaauaacacc gaacuccccu cgggcagaag ccacacucgg gggauucgga     1140

agccuaggcc uagacugcga gcccagaacc ggacuggacu ucagcgaccu cuacuaccua     1200

acaaugaaca acaagcacug gcuaguacac aaagaauggu uccacgacau accucuccca     1260

uggcacgcag gggcagacac aggcacaccc cacuggaaca auaaagaagc ccuaguggag     1320

uucaaagacg cccacgcaaa aagacaaacc guaguagucc ucggaagcca agaaggcgcc     1380

guccacaccg cgcuagccgg ggcgcuggaa gcggaaaugg acggcgccaa aggacgacua     1440

agcagcggcc accuaaaaug ccgacuaaaa auggacaaac uaaggcucaa aggcguaucc     1500

uacucacucu gcacagccgc cuucaccuuc acaaaaauac cagcagagac gcuccacggc     1560

acagugacgg uggaagucca auaugccggc acagacggac caugcaaggu gccagcacaa     1620

auggcggucg acaugcaaac acuaacaccc guaggccgcc uaauaaccgc caaccccgua     1680

aucaccgaaa gcacggaaaa uucgaaaaug augcuagagc ucgacccacc guucggagac     1740

ucauacaucg ucauaggagu gggcgagaaa aagauaacac accacuggca ccgaagcggc     1800

uccacaaucg gaaaagcauu cgaagccaca guaagagggg ccaagagaau ggccguacuc     1860

ggagauacag caugggauuu cgggucagua ggcggagccc uaaacagccu aggcaaaggc     1920

auccaccaga ucuucggagc agcauucaaa agucucuuug gaggaauguc cugguucucc     1980

caaauacuga ucggcacacu acugaugugg cugggacuaa acaccaaaaa cgggagcauc     2040

ucacucaugu gucuggcacu gggcggagua cucaucuucc uaagcacagc cguaucagca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  5041
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5041
gggagaaagc uuaccaugaa aagacgcggc gcggacaccu ccgucggcau agucggucuu       60

cugcucacaa cagcaauggc cgcagagguc acacgaagag gcagcgccua cuacauguac      120

cucgaccgaa acgacgcagg cgaagcaauc ucauucccca caacccuagg caugaacaaa      180

ugcuacaucc agauaaugga ccuaggccac augugcgacg ccacaauguc cuacgaaugc      240

ccuauguuag acgaaggagu ugaaccagac gacguagacu gcuggugcaa cacaacauca      300

acgugggugg uguauggcac augccaucac aagaaaggag aagccagacg cagcagacga      360

gcagucaccc uacccagcca cucaacacga aaacuccaaa cacggucaca gacauggcuc      420

gaaagccgcg aauacacgaa acaccuaauc cgggucgaga auuggaucuu ccgcaaucca      480

ggauucgccc ucgcagccgc agccaucgcc uggcuacuag gaagcuccac cucacaaaaa      540

gucaucuacc ucgucaugau acuccuaaua gcaccagccu acuccaucag augcauaggc      600

gucagcaacc gcgacuucgu agaaggaaug aguggcggca ccugggucga caucguccua      660

gaacacggcg gaugcguaac cgugauggcg caggacaaac ccaccgugga cauagagcua      720

gucaccacca ccgucucgaa cauggcagaa guacgauccu acugcuacga agccagcaua      780

agcgacaugg caagcgacag ucgcugcccc acccaaggag aagccuaccu agauaaacaa      840

agcgacaccc aauacgucug uaaacgaacc cuagucgacc gcggaugggg caacggcugu      900

ggacucuucg ggaaaggcuc acuggugaca ugcgccaaau ucgcgugcuc uaagaaaaug      960

acagggaaau cuauccaacc cgaaaaccua gaauaccgca ucaugcuaag cguacacggc     1020

ucacaacacu ccggcaugau aguaaacgac acaggacacg aaaccgacga gaacagagcc     1080

aaaguagaaa ucacgcccaa cucccccagg gccgaagcaa cccucggcgg cuucggaucc     1140

cuugggcuag acugcgaacc cagaaccgga cucgacuuca gcgaccucua cuaccuaaca     1200

augaacaaca aacacugguu agugcacaag gaaugguucc acgacauccc acuaccaugg     1260

cacgcgggag cagacacugg cacaccccac uggaacaaca aagaagcucu cgucgaguuc     1320

aaggacgccc acgccaaaag gcaaacaguc gucguccuag ggagccagga aggagccgug     1380

cacaccgcac ucgcuggcgc ccuggaagcc gaaauggacg gagccaaagg cagacuauca     1440

agcggacacu uaaaaugcag acuaaaaaug gauaaacucc gucuaaaagg cguaagcuac     1500

agccuaugca ccgccgcguu cacauucacc aaaauaccag cagaaacucu ccacggcacc     1560

gugacaguag aaguccagua cgccggcacg gauggaccau guaaaguacc ggcccaaaug     1620

gcaguagaca ugcaaacacu cacacccgua ggacgccuaa ucaccgcaaa ccccgugaua     1680

accgaaucaa ccgaaaacag caaaaugaug cucgaacucg acccaccauu uggagauagc     1740

uacaucguga ucgggguagg agagaaaaag aucacgcacc auuggcaccg cucaggcuca     1800

accauaggaa aggccuucga agcaaccgua cgaggagcaa aacgcauggc aguacuagga     1860

gacacugccu gggacuucgg cucaguaggc ggagcacuca acucccuagg caaaggaaua     1920

caccaaaucu ucggcgccgc cuucaaaagc cucuucggcg guauguccug guucagccaa     1980

auccugaucg gcacacuucu aaugugguua ggacucaaca ccaaaaacgg cuccaucucc     2040

cuaaugugcc uagcacucgg cggcguacuc aucuuccuaa gcacagcagu guccgcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  5042
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5042
gggagaaagc uuaccaugag acgaggcgcc gacacaucag uuggaauagu aggacuacua       60

cuaaccacag ccauggccgc agaaguaaca agacgcggau ccgccuauua cauguaccua      120

gaccgaaaug acgcaggcga ggcgaucagc uucccaacaa cgcugggaau gaacaaaugc      180

uacauacaaa uaauggaccu uggccacaug ugugacgcaa caaugagcua cgaaugcccc      240

augcucgacg agggcgucga accagacgac guagacugcu ggugcaauac aacaagcacc      300

uggguagucu acggaacaug ccaccacaaa aagggagaag cacgacgcag ccgccgagcg      360

guaacccucc ccagccauuc gacccggaaa cuacagaccc gaagccaaac cuggcuagaa      420

ucacgcgaau acaccaaaca cuuaaucaga guggagaacu ggaucuuccg caaccccgga      480

uucgcacuag cagcagccgc aauagcaugg cuccuaggca gcagcacaag ccaaaagguc      540

auauaccucg ugaugaucuu acucauagcc ccagccuaca gcauacgaug cauaggcgug      600

uccaacagag acuucgucga aggcaugagc ggaggcacau ggguagacau aguacucgaa      660

cacggcggau gcguaacggu gauggcccaa gacaaaccaa caguagauau cgaacugguc      720

accaccacag ucagcaauau ggcagaagua agauccuacu gcuacgaagc auccauaagc      780

gacauggcaa gcgauagcag augccccaca cagggcgaag ccuaccucga caaacaauca      840

gauacacaau acgucugcaa gcgcacccug gucgacagag gcuggggaaa uggaugcggc      900

cuauuuggaa aagggucccu ggucacgugc gcaaaguucg cgugcagcaa gaaaaugacc      960

ggcaaaagca uccaacccga gaaccucgaa uacagaauaa ugcucagcgu gcacgggagc     1020

caacacagcg gcaugauagu aaacgacacu ggacacgaaa ccgaugagaa ccgagccaaa     1080

gucgaaauaa ccccuaacuc accacgagca gaagcgacac uagggggcuu cggcagucuc     1140

gggcuagacu gcgagcccag aaccggccuc gacuuuuccg accucuacua ccuaaccaug     1200

aacaacaaac acuggcucgu gcacaaagaa ugguuccacg acaucccacu cccauggcac     1260

gcaggagcag acacaggaac accccacugg aacaacaaag aggcacucgu cgaguucaag     1320

gacgcccacg caaaaagaca aacaguagua guucucggaa gccaagaagg agcaguacac     1380

accgcccuug caggggcccu cgaagccgag auggacggag cgaaaggccg gcuaucguca     1440

ggccaccuca aauguagacu aaagauggac aaacucagac uaaaaggagu gucauacagc     1500

cugugcacag ccgcguucac auucacaaaa auaccggcgg aaacacucca cgggacgguc     1560

accguagagg ugcaauacgc cggcaccgac ggccccugca aagucccggc acagauggcc     1620

gucgacaugc aaacccucac accaguaggc cgacuaauca cagccaaccc gguaauaacg     1680

gaauccacag aaaacucaaa aaugaugcua gaacucgacc caccauuugg cgacagcuac     1740

auagugauag ggguaggcga aaagaaaauc acacaccacu ggcaccgaag cggcagcacc     1800

auagggaaag cauucgaagc aaccguacgg ggagcaaaac gcauggcagu acucggggac     1860

acagccuggg acuucggcag cguaggcggu gcacuaaacu cacuaggcaa gggcauacac     1920

caaauauucg gggcggccuu caaaucucua uucgggggca ugagcugguu cucacaaauc     1980

cuaaucggga cccuccucau guggcuagga cucaauacaa agaacggcag cauaucgcuc     2040

augugccugg cauuaggagg aguccuaauc uuccucagca cagcaguaag cgcaugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  5043
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5043
gggagaaagc uuaccaugcg cggagcagac accagcgucg gcaucguggg ccuccuucug       60

acaacagcca uggccgcgga gguaacacga agaggcagcg cguacuacau guaccuagac      120

agaaaugacg caggagaagc caucucguuc ccaacaaccc uaggaaugaa caaaugcuac      180

auccaaauca uggaccuagg ccacaugugc gacgccacca ugagcuacga augcccaaug      240

cucgacgaag gaguagagcc cgacgacgua gacugcuggu gcaacaccac cagcacaugg      300

guaguauacg gaaccugcca ccacaaaaag ggggaagcuc gccgaagccg ccgcgcuguc      360

acacucccca gccacagcac cagaaaacuc caaacccgcu cacaaaccug gcuagaaagc      420

cgagaauaca ccaaacacuu aauccgagug gaaaacugga uauucaggaa ucccggcuuc      480

gcccuugccg ccgccgcaau agccuggcuc cucggcagcu caaccucaca aaaagucaua      540

uaccuaguca ugauacuccu aauagccccc gccuacucca uaagaugcau aggcguaagc      600

aacagagacu ucguagaagg gaugucuggc ggcaccuggg uagacauugu acucgaacac      660

ggcggcugug ugacagugau ggcccaagac aaaccaaccg uagacaucga gcuagucaca      720

accaccgucu ccaacauggc cgaagucaga uccuacugcu acgaagcgag cauauccgac      780

auggcguccg acagccgaug ccccacacag ggcgaagccu accucgacaa acaaagcgac      840

acccaguacg uauguaaacg caccuuagug gacagaggau ggggcaacgg cugcggacuc      900

uucggcaagg gaucccucgu cacaugcgcc aaauucgcau gcucgaagaa aaugaccggc      960

aaaagcaucc aaccagaaaa ccucgaauac cgaauaaugc ucucgguaca cggcagccaa     1020

cacagcggaa ugaucguuaa cgacaccggg cacgagacag acgaaaaccg ugccaaaguc     1080

gaaauaaccc ccaacagccc aagagcagag gccacccucg gcggauucgg uagccuagga     1140

cuagacugcg agcccagaac cggccuggac uucucagacc ucuacuaccu aacuaugaac     1200

aacaaacacu ggcuagucca caaagaaugg uuccacgaca uaccccuacc cuggcacgcc     1260

ggagcagaca caggcacccc acacuggaac aacaaagaag cccuaguaga guucaaggac     1320

gcacacgcaa aacgccaaac aguuguaguc cuuggcagcc aagaaggagc cguccacacc     1380

gcccucgcag gagcccuaga agcagaaaug gauggcgcga aaggccgccu aucauccggc     1440

caccuaaaau gcagacucaa gauggacaaa cuaagacuca agggagucuc cuacucccua     1500

uguaccgcag ccuucacguu caccaaaauc ccagcagaaa cacuccacgg caccguaacc     1560

gucgaaguac aauaugccgg uacagacgga cccugcaagg uaccagccca aauggccgug     1620

gacaugcaaa cccuaacacc cguaggccgc cugaucacag cgaaccccgu uaucaccgaa     1680

uccacagaaa acucaaagau gaugcucgaa cuagacccac ccuucggaga cuccuacaua     1740

gucaucggcg ucggggaaaa gaaaauaaca caccacuggc accgcuccgg cagcaccaua     1800

gguaaagcau ucgaggccac cguacgagga gccaaacgaa uggccguacu aggcgacacc     1860

gccugggacu uuggauccgu gggcggcgca cuaaacucac uaggcaaagg aauacaccaa     1920

aucuucgggg cagccuucaa aagccuauuc ggcggaaugu cuugguucuc acaaauccuc     1980

auaggaacac uccugaugug gcucggacug aacaccaaaa acggauccau aagucuaaug     2040

ugccuagcac ugggcggagu ccuaaucuuc cugagcaccg caguauccgc gugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  5044
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5044
gggagaaagc uuaccauggg cgccgacaca ucggucggaa uagucggacu acuccucaca       60

acagccaugg cagcggaagu cacgcgaaga ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgcag gggaagcgau cagcuucccc accacccucg ggaugaacaa augcuacaua      180

caaauaaugg aucuaggaca caugugcgac gcgacgauga gcuacgaaug ccccaugcua      240

gacgaaggag uagaaccaga cgacgucgac uguuggugca acacaacauc cacuugggug      300

guauauggaa ccugccauca caagaaagga gaagccagga ggucccgcag agccgucaca      360

cuccccagcc acuccacucg caagcuacaa acaagaucac aaaccuggcu agaaagcagg      420

gaauauacaa aacaccuaau aagaguggaa aacuggaucu ucagaaaccc cggcuucgcc      480

cuagccgcag cggccauagc cuggcuacuc ggcagcucca caagccagaa agucaucuac      540

cucguaauga uccuucugau cgccccagcg uacuccauac gguguauagg aguauccaac      600

cgcgacuucg ucgaaggcau gucaggcgga acaugggucg acauaguccu cgaacacggu      660

ggaugcguca cgguaauggc acaggacaaa cccacggucg auaucgagcu aguaacaacc      720

acggucucca acauggccga aguccgcuca uacugcuacg aagccuccau auccgacaug      780

gcguccgacu cccgcugccc aacccaaggc gaagccuacc ucgacaaaca auccgacacc      840

caauacguau gcaaacgaac cuuagucgac cgcggauggg ggaacggaug uggccuauuc      900

ggcaagggcu cgcucguuac augcgccaaa uuugccugcu cgaagaaaau gacgggaaaa      960

agcauccaac ccgaaaaccu agaauacaga auaaugcuau cgguccaugg aucacaacac     1020

ucagggauga uaguaaacga cacgggacac gagaccgacg aaaaccgcgc gaaagucgaa     1080

auuaccccaa acaguccucg cgcagaagca acccuaggcg gguucggaag ccucggccuc     1140

gacugcgaac cacggacagg cuuagacuuu agcgaccugu acuaccucac caugaacaac     1200

aaacacuggu uaguccacaa agaaugguuc cacgacauac cccuaccaug gcacgccgga     1260

gccgauacag guacacccca cuggaacaac aaggaagcgc uaguggaguu caaagacgcc     1320

cacgcaaaac gccaaaccgu cgucgucuua ggcagccaag aaggagccgu ccacaccgcg     1380

cucgccggag cccuagaagc agagauggac ggagcaaaag gacgccuaag uucagggcac     1440

cuaaagugcc gacugaaaau ggacaagcug cgucucaaag gcguguccua cucacuaugc     1500

acggcggcau uuacguucac caaaaucccc gcagaaaccc uacacggaac cgucaccgua     1560

gaaguccaau augccggaac ggacggaccc ugcaaggucc cagcccaaau ggccgucgac     1620

augcagaccc ugacccccgu aggccggcua auaaccgcaa acccgguaau aacagaauca     1680

acagagaacu caaaaaugau gcucgaacug gacccacccu ucggcgacuc cuacauaguc     1740

aucggaguag gagaaaagaa aauaacccac cacuggcacc gcagcggaag caccauaggc     1800

aaagcguucg aggcgaccgu ccgcggcgcc aaaaggaugg caguccuagg agacaccgca     1860

ugggacuucg gaagcguagg aggcgcccuc aacucacuag gaaaaggaau ccaccaaauc     1920

uucggagcgg cguucaaaag ccucuucggc ggaaugagcu gguucucaca aauccucauc     1980

ggaacccugc uaauguggcu cggacuaaac accaaaaacg ggagcaucuc ccuaaugugu     2040

cucgcacuag guggcguccu caucuuccuc agcacagcag uaucagcgug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  5045
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5045
gggagaaagc uuaccauggc cgacacaagc guaggaauag uaggacugcu ccugaccaca       60

gcaauggcag cagaaguaac aagacgaggc uccgcguauu acauguacuu agaccgaaac      120

gacgccggag aagccauauc uuuccccacc acccuaggga ugaacaagug cuacauucaa      180

auaauggacu ugggccacau gugcgaugcg accaugagcu acgagugccc caugcuagac      240

gagggcgucg aaccagacga cguagacugc uggugcaaca cgaccucaac auggguagua      300

uacgggacau gccaccacaa gaaaggagag gcacgaagaa gccgacgagc cguaacgcua      360

cccucccacu cgacacgcaa acuccaaaca agaucccaaa cauggcugga aagcagggaa      420

uacacaaaac accucauuag aguagaaaac uggauauuuc gaaacccagg cuucgcccua      480

gcagcagcag cgaucgccug gcuccucgga agcagcaccu cgcaaaaagu aauuuaccuc      540

guaaugauac uacuaaucgc gccagcauac agcaucaggu guaucggagu aagcaacaga      600

gacuucguag aaggcauguc aggaggaacc uggguagaca ucguacuaga acacggagga      660

ugcguaaccg ugauggcuca agacaaacca accguagaca ucgagcuagu aaccacuaca      720

gucuccaaua uggcugaagu cagauccuac ugcuacgaag ccucaauuag cgacauggcg      780

agcgacagcc gaugcccgac ccaaggagaa gccuaccucg acaaacaauc ggacacccaa      840

uacguaugca aaaggacacu ggucgaccga ggcuggggga acggcugcgg ccuguucggg      900

aaaggcagcc ucguaacaug cgcgaaauuc gccugcucca agaaaaugac uggaaaauca      960

auccaaccag aaaaccugga auacagaauc augcucuccg uacacgggag ccaacacagc     1020

ggaaugaucg uaaacgacac cggccacgaa accgacgaaa accgcgcgaa agucgaaaua     1080

acgcccaacu cacccagagc cgaggccaca cuaggagguu ucggaucacu agggcucgac     1140

ugcgaaccca ggaccggacu agacuucagc gaccucuacu accucacaau gaacaacaaa     1200

cacuggcuag ugcacaaaga augguuucac gacauacccc uacccuggca cgcaggcgca     1260

gacaccggca cgccccacug gaacaacaaa gaagcacucg ucgaguucaa agacgcccac     1320

gccaaacgcc agaccguagu cguccuagga agccaagaag gagcagucca caccgcccua     1380

gcaggggcuc uagaagccga aauggacggc gcaaagggac gacucagcag cggccaccua     1440

aaaugccgcc ugaaaaugga caaacucaga cucaaaggag uaucauacuc acuaugcaca     1500

gcagcauuca cauucaccaa aauccccgca gaaacccucc auggaacagu cacagucgaa     1560

guucaauaug ccggcacaga cggaccaugc aaaguacccg cacaaauggc cguagacaug     1620

cagacgcuaa cccccgucgg aaggcucaua acagcuaacc ccgucauaac cgaaagcaca     1680

gaaaacucca aaaugaugcu cgaacucgau cccccguucg gcgacucgua cauugucaua     1740

ggcgucggag aaaagaaaau cacacaccac uggcaccgcu caggcagcac aaucggcaaa     1800

gccuucgaag cgacaguacg gggagccaaa cgaauggccg uccucggaga cacagccugg     1860

gacuucggaa gcgucggagg agcccuuaac agucugggaa aaggaaucca ccaaaucuuc     1920

ggggcagcuu ucaaaagccu cuucggcggc augagcuggu ucagccaaau ccuaaucgga     1980

accuuacuaa uguggcucgg gcugaacacg aaaaacgggu ccauaagccu caugugccuc     2040

gcccugggcg gaguccucau auuccuaagc acagccgucu ccgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  5046
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5046
gggagaaagc uuaccaugga cacaagcguc ggaauagugg gucuccuacu cacgaccgca       60

auggccgcag agguaacccg aaggggcucu gcauacuaca uguaccugga ccgcaacgac      120

gcaggcgaag ccauaucauu ccccacgaca cucggaauga acaaaugcua uauacagauc      180

auggaccucg gacauaugug cgacgccacc auguccuacg aaugccccau gcuagacgaa      240

ggcguggaac cagacgacgu cgacugcugg uguaacacca cgagcaccug gguagucuac      300

ggcacaugcc accacaagaa aggggaagcc agaagaucac gccgagcggu aacgcucccg      360

ucacacucca caagaaaacu acagacacga agccaaaccu ggcucgaaag ccgcgaauac      420

accaagcacc uaauaagagu agagaacugg auuuuccgaa acccuggguu cgcgcuagca      480

gcugcagcaa uagccuggcu ccuagggagc ucaacgucac agaaagucau cuaccucguc      540

augauacuac ugaucgcacc cgccuacucc auccgaugca uaggcgucuc aaaccgcgac      600

uucguagaag gcauguccgg agggaccugg gucgauaucg uucucgaaca cggaggaugc      660

guaacaguca uggcccagga uaaacccaca guagacauag aacucguaac aacgacaguc      720

ucaaacaugg ccgaaguaag guccuacugc uacgaagcau caaucucgga cauggccucc      780

gacucacgau gccccaccca aggcgaagcc uaccucgaca aacaaucaga cacccaauau      840

gucuguaaac ggacccucgu agaccgcggc uggggcaacg gaugcggccu auucggcaaa      900

ggcucccuag ugaccugcgc caaauucgca ugcucaaaga aaaugacagg caaaagcaua      960

caacccgaaa acuuagaaua ccguaucaug cuuuccguac acggcagcca acacuccggc     1020

augaucguca augacaccgg ccacgaaacu gacgaaaaua gagcaaaagu agaaaucaca     1080

ccaaacagcc cacgagcgga agcgacgcuc ggcggcuuug gcucacucgg ccucgacugu     1140

gagccaagaa caggacuaga cuucuccgau cuauacuacc ucacgaugaa caacaaacau     1200

uggcuaguac acaaagaaug guuucaugau aucccccuac cuuggcacgc cggagccgac     1260

acaggaacac cucacuggaa caauaaagag gcacucguag aguucaaaga cgcacacgcc     1320

aaaagacaaa ccguagucgu ccuuggcuca caagaaggcg ccguacacac cgcccucgcc     1380

ggagcacucg aagccgagau ggacggcgcc aaaggccgac uaucuagcgg ccaccucaag     1440

ugcaggcuca agauggauaa acuacgacuc aagggaguau cauacucccu augcaccgca     1500

gcauucacgu ucacaaaaau acccgcagaa acccuacacg gcacagucac aguggaggua     1560

caguacgccg gcacagacgg accauguaaa gucccagcac agauggccgu ugacaugcag     1620

acccuaaccc ccgucgggag acucaucaca gcaaacccag uaaucacaga aagcacagaa     1680

aacucaaaga ugaugcucga acuugaccca cccuucggcg acuccuacau cgucauagga     1740

guaggagaga agaaaauaac ccaccacugg caccgcuccg gaaguacaau aggcaaagca     1800

uucgaagcca ccguccgagg cgcaaaacgc auggcggucc uaggcgacac cgcgugggac     1860

uucggaagcg ucggcggcgc acucaacucu cugggaaaag gcauacacca gauuuucggc     1920

gcagccuuca aaucccuauu cggcggcaug agcugguuca gccaaauacu caucggcacu     1980

cuccuaaugu ggcuaggccu caacacgaaa aacggcagca uaagccuaau gugccucgcc     2040

cuaggaggag uccuaauauu ccuaucaaca gcagugagcg cuugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  5047
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 1 opt

<400>  5047
gggagaaagc uuaccaugac cagcgucggg aucgucggcc uccuacuaac cacagcaaug       60

gcagcagaag uaaccaggcg cgguuccgcc uacuauaugu accucgacag aaacgacgcg      120

ggcgaagcca ucuccuuucc aaccacacuc ggcaugaaca aaugcuacau ccaaauaaug      180

gaccucggac acaugugcga cgccacgaug uccuacgaau gucccaugcu agacgaaggu      240

gucgaaccag acgacguaga cugcuggugc aacaccacca gcaccugggu gguauacggc      300

acaugccacc acaagaaagg cgaagccaga cgaucgcgcc gagccgucac gcuccccucc      360

cacucgacca gaaaacuaca aacccgcucu cagaccuggc ucgaaucgcg agaauauacc      420

aaacaccuua uacgagucga aaacuggaua uuccgcaacc caggauuugc auuagccgcc      480

gcagcaaucg ccuggcuacu cgggagcuca acaucacaaa aaguaauuua ccucgucaug      540

auccugcuca uugccccagc uuacucaaua cgauguaucg ggguaagcaa ccgagacuuu      600

gucgaaggca ugagcggagg gaccuggguc gacaucgucc ucgaacacgg cggcugcgua      660

acagucaugg cccaggauaa acccaccguc gacauugagc uagucacaac cacagucucg      720

aacauggccg aaguacgcag cuacugcuac gaagcgucca uaagugacau ggccagcgac      780

ucccgcugcc ccacucaagg cgaagccuac cucgauaaac aaagcgacac ccaauacgua      840

ugcaaaagaa cccucgucga ccgaggcugg ggcaacgguu gcggccuauu cggaaaaggc      900

ucccuaguua ccugcgcaaa guucgcaugc ucaaagaaaa ugaccggcaa auccauccaa      960

ccagaaaacc uggaauaccg cauaaugcug agcguccacg gcucccaaca cuccggaaug     1020

aucgucaacg acacagggca cgaaacggac gaaaaccgcg caaaaguuga aauaaccccc     1080

aauagccccc gcgcagaagc aacacucggc ggcuucggaa gccuagggcu cgacugcgaa     1140

ccccgcacag gccucgacuu cuccgaccug uacuaccuaa ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacauc ccacuaccau ggcacgcggg ugcagacaca     1260

ggcacacccc acuggaauaa caaagaagcc cucguagagu ucaaagaugc ccacgcaaaa     1320

agacagaccg ucguaguccu cggcagucaa gaaggcgccg uccacacggc ucucgccgga     1380

gcccuagaag ccgaaaugga cggagcaaaa ggaaggcuau ccucaggcca ccucaaaugc     1440

aggcucaaga uggacaaacu aagacucaaa ggcguaagcu acagccucug cacagccgca     1500

uucaccuuca cgaaaauucc cgcggaaacg cuacacggca cagugacagu cgaaguccag     1560

uacgcaggca cagacgggcc augcaaagua cccgcacaaa uggcggucga caugcagaca     1620

cucacccccg uaggaagacu uaucacagca aaccccguaa ucacagaauc cacagaaaac     1680

agcaaaauga ugcuagaacu agauccaccc uucggagacu ccuacaucgu aauaggcguc     1740

ggagaaaaga aaaucacuca ccauuggcac agaagcggcu ccacaauagg gaaagccuuc     1800

gaggcgacag uacgcggagc uaaacgcaug gccguacucg gggacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcacu aaacagucuc ggcaaaggaa uacaccaaau cuucggcgcc     1920

gccuucaaau cccuauucgg aggcaugagc ugguucagcc aaauccugau aggcacauug     1980

cucauguggu uaggccucaa caccaaaaac ggcagcauca gccucaugug ccucgcacuc     2040

gggggaguac uaauauuccu auccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5048
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5048
gggagaaagc uuaccaugac cuccguaggc aucgugggac uccuccucac aaccgcaaug       60

gccgcagccg uaaccagacg cggcuccgca uauuacaugu accucgacag aaacgacgcc      120

ggcgaagcga ucucguuucc caccacccuc gggaugaaca aauguuacau ccaaaucaug      180

gaccucggcc acaugugcga cgccaccaug agcuacgaau gccccaugcu agaugaagga      240

guagaaccag acgacguaga cugcuggugc aacaccacau caacaugggu aguguacggc      300

acaugucacc acaagaaagg ugaagcacgg cgcagcagac gcgcgguaac gcuccccuca      360

cacuccacac gcaaacucca aaccagaagc caaaccuggc uagaaagccg agaauacacc      420

aaacaccuca uacgagucga aaacuggaua uucagaaacc ccggauucgc ccucgcggcc      480

gcggcaauag cuuggcuacu cggauccucg accucacaaa aagucaucua ccuggucaug      540

aucuuacuca uagcaccagc auacucgauc cgguguaucg gcgugucuaa ccgcgacuuu      600

gucgagggca uguccggcgg caccugggug gacaucgucc uagaacacgg gggcugcguc      660

accguuaugg cccaagacaa acccacggua gacauagagc ucgucaccac cacaguaucc      720

aacauggccg aaguccgauc auacugcuac gaagccucca uaagcgacau ggccucagac      780

ucccgcugcc ccacacaagg ugaagcauac cuagacaaac aaucagacac gcaauacgug      840

ugcaaacgca cucuggucga ccgaggcugg ggcaacggcu gcggccucuu cggcaaggga      900

agccuaguaa ccugcgccaa auucgccugc ucaaagaaaa ugaccggcaa aucaauccaa      960

cccgaaaacc ucgaauaccg aaucaugcua ucaguccacg gaucacagca cucuggcaug     1020

auagucaacg acacagguca cgaaacagac gaaaaccgcg ccaaaguuga aaucaccccc     1080

aacucaccuc gagcagaagc cacccucgga ggauucggcu cccuaggccu agacugcgaa     1140

ccacgcacag gacuagacuu cagcgaccuc uauuaccuca ccaugaacaa caaacauugg     1200

cuaguucaca aagaaugguu ccacgacaua ccccucccau ggcaugccgg ugcagacacc     1260

ggaacaccuc acuggaacaa caaggaagcc cucguagagu ucaaggacgc acacgcaaaa     1320

cgacaaacag ucguaguccu cggaagccag gaaggagccg uucacaccgc acuugcagga     1380

gcccuggaag ccgaaaugga cggugccaaa ggccgccuuu ccucagggca ccucaaaugc     1440

cgccucaaaa uggacaaacu acgccucaaa ggcgucucuu acucccucug uacugccgcc     1500

uuuaccuuca ccaagauacc agcagaaacu cugcacggca ccgucacagu cgaaguacaa     1560

uacgcuggca ccgacggacc cugcaaaguc cccgcccaaa uggccgucga caugcagacc     1620

cuaacccccg uaggaagacu aaucacagcc aauccaguca ucaccgaauc cacagaaaac     1680

ucaaaaauga ugcucgaacu cgauccaccc uucggagaca gcuacauugu caucggcguc     1740

ggugaaaaga aaauaacaca ccacuggcau cgaucaggcu caacgaucgg aaaagcguuc     1800

gaagcuacag uacgaggcgc caaacggaug gccguccucg gcgacaccgc uugggacuuc     1860

ggcuccgucg gaggugcacu caacucacuc ggcaaaggua uccaccaaau cuucggcgca     1920

gcguucaaga gccucuucgg cggcauguca ugguuuucac aaauacucau aggaacacua     1980

cucauguggc ucgggcucaa cacaaagaac ggcagcauaa gccucaugug ccucgcacuu     2040

ggcggcgucc uaaucuuccu aucgacagca guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5049
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5049
gggagaaagc uuaccaugcg cggcgcggac acaucagucg gcaucgucgg gcuacuacuc       60

acgacggcca uggcagccgc ugucacaaga cgaggaagug ccuauuacau guaccucgac      120

cgcaacgacg ccggggaagc caucuccuuc cccacaacac ucggcaugaa caaaugcuac      180

auccaaauca uggaccucgg ccauaugugc gacgccacca ugucauacga gugcccaaug      240

cuagacgaag gagucgagcc agacgacguc gacugcuggu guaacacaac cuccacaugg      300

guaguuuaug gcacguguca ccacaaaaag ggcgaagccc gccgaagccg cagagccguc      360

acccuaccuu cacacagcac ccgcaagcua caaacacgca gccaaaccug gcuggaauca      420

cgagaauaca caaagcaccu aauacgcguc gagaacugga uauuccgcaa ccccggcuuc      480

gcacuagcgg ccgcagcgau cgcauggcuc cucggcucca gcacaucgca aaaggucauc      540

uaccucguaa ugauacuccu aaucgcaccc gcguauagca uacgguguau aggcgucagc      600

aaccgagacu ucguagaagg caugagcggu gguacauggg ucgacaucgu ccucgaacac      660

ggaggcugcg ucacagucau ggcacaagac aagccaacag uagacaucga acuuguaacc      720

accaccguua gcaacauggc ugaaguuaga ucauacugcu acgaagccag cauaagcgac      780

auggccucag acagccgaug cccaacgcaa ggagaagccu accuagacaa acaaucggac      840

acccaauacg ucugcaaacg aacccuaguc gaccgcggcu ggggaaaugg augcggccua      900

uucggaaaag gaucccucgu cacaugcgcc aaauucgcgu gcucaaagaa aaugaccggc      960

aaaagcauac aaccagaaaa ccuagaauac agaauaaugc ucucagucca cggcagccaa     1020

cacagcggca ugaucgucaa cgacacaggc cacgaaaccg acgaaaacag agcaaaaguc     1080

gagauuacac cuaacagucc acgcgcugaa gccacccuag gcggauucgg aucgcuaggc     1140

cucgacugcg aaccacguac cggacuagac uucuccgacc uauacuaccu cacaaugaac     1200

aacaaacacu ggcucguaca caaagaaugg uuccacgaca uaccccuacc cuggcacgcc     1260

ggagcagaca ccgggacacc acacuggaac aacaaagaag cccucgucga guucaaagac     1320

gcccacgcaa aacgccaaac agucgucgua uugggcuccc aagaaggggc aguccacaca     1380

gcccucgcgg gagcccuaga agcagaaaug gacggcgcca aaggacgacu aagcuccggc     1440

caccuuaagu gcagacucaa aauggacaaa cugcgacuua aaggagucuc auacucacuc     1500

ugcacagcag cuuucacauu cacaaaaaua ccagcagaaa cccuucacgg cacagugacg     1560

guagaagucc aguacgccgg aaccgacggc cccugcaaag uacccgccca aauggccgua     1620

gacaugcaaa cgcuaacccc aguuggacgu cucauaacag cuaacccagu aaucaccgaa     1680

ucgacugaaa acucgaagau gaugcuagaa cucgacccac cauucggcga cagcuacauc     1740

guaaucggcg ucggcgagaa aaagauaacu caucacuggc accgcagcgg uagcacaauc     1800

ggcaaagccu ucgaagccac gguaagggga gcaaaacgca uggcaguccu gggggacacc     1860

gcaugggauu ucggaucggu gggcggggcc cuaaacucac ugggcaaagg aauccaccag     1920

aucuucggcg ccgcuuucaa aucacucuuc ggcggcaugu ccugguucuc acaaauacuc     1980

aucggaacac uacucaugug gcucggacuc aacaccaaaa acggaagcau aagccucaug     2040

ugccucgcac uaggaggagu ccucauuuuc cucucaaccg ccguuuccgc augaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  5050
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5050
gggagaaagc uuaccaugcg cggccugagc agcagaaaac gacgaucaca cgacguacuc       60

acaguccaau uccucauacu cggaaugcua cucaugaccg gcggggucac gagaagaggc      120

agcgcauacu acauguauuu agaccgaaac gacgccggcg aagccaucuc cuuccccacc      180

acacugggca ugaacaaaug cuacauccaa aucauggacc uaggacacau gugcgacgcg      240

accaugucau acgaaugccc gaugcucgac gaaggcgucg aaccagacga cgucgauugc      300

uggugcaaca ccacauccac cuggguagua uacggcacau gccaccacaa gaaaggagaa      360

gcccgacgca gccggcgcgc ugucacccuc ccgucacacu cuacccgaaa gcugcaaacc      420

cgaucacaga ccuggcugga aucacgcgaa uacacaaaac accucauccg cgucgaaaac      480

uggauauucc gcaaccccgg cuucgcccua gcagcugcag ccaucgccug gcuacucggc      540

uccagcacca gccaaaaggu caucuaccuc guaaugaucc uccucauagc cccagcauac      600

ucaauccgau gcaucggagu gagcaaccgu gacuucgucg aaggaauguc cggcgggaca      660

ugggucgaua uugugcucga acacggaggc ugcgucacag uaauggccca agacaaacca      720

accguagaca uagagcucgu uaccaccacc guauccaaca uggccgaagu gcgcuccuac      780

ugcuacgaag caagcaucag cgacauggcc uccgauucaa gaugccccac gcaaggcgaa      840

gcauaccuag acaaacaauc agacacacaa uacgucugca aacgaacccu cgucgacagg      900

ggauggggga acggcugcgg ccuuuuuggg aaaggauccc uagucaccug ugccaaauuu      960

gcaugcagca aaaagaugac cggaaaauca auucaaccag aaaaccucga auaccgaaua     1020

augcucucag uccacggcag ccagcacagc ggaaugaucg uaaacgauac aggccacgaa     1080

acagacgaaa accgcgccaa aguagaaaua acacccaacu caccccgcgc agaagccaca     1140

cuagguggau ucgggagccu aggacuagac ugcgaacccc gcacaggucu agacuucucc     1200

gaccucuacu accucacaau gaacaacaag cacuggcuag uccacaaaga augguuccac     1260

gacaucccgc uaccauggca cgccggcgcc gacaccggaa caccccacug gaacaacaaa     1320

gaagcccuag ucgaguucaa agacgcacac gcaaagagac aaacugucgu aguccucggc     1380

ucccaagaag gcgccgucca uaccgcacuc gcaggcgcau uagaagccga gauggacggc     1440

gcaaaaggac gacuaagcag cggacaccuc aaaugccgcc uaaaaaugga caaacugaga     1500

cucaaaggag ucucuuacuc acucugcacc gccgcauuca ccuucacgaa aauccccgcc     1560

gaaacccucc acgguaccgu uaccgucgaa guccaauaug cagggacaga cggcccaugc     1620

aaaguccccg cccaaauggc agucgacaug cagacccuca cgccagucgg cagacuaauc     1680

acagcaaacc ccgucauaac cgaaagcacg gaaaacucca aaaugaugcu cgaacuagau     1740

cccccauucg gcgacucaua caucgucauc ggaguaggug agaaaaagau cacccaccac     1800

uggcacagaa gcggaagcac gaucgguaaa gccuucgaag ccacuguccg aggugccaaa     1860

cgcauggcug ugcucggaga caccgccugg gacuucggga gcgugggcgg agcccuaaac     1920

agccuaggga aaggcauaca ccagaucuuc ggcgcagcau ucaagagcuu auucggcggg     1980

augagcuggu ucucacaaau ccucaucgga acacuccuca uguggcuagg acucaacacc     2040

aaaaacggaa gcauaagucu gaugugccuc gcccucggag gaguccuuau auuccuaucc     2100

acagccguca gcgcgugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  5051
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 1 opt

<400>  5051
gggagaaagc uuaccaugag aggccuauca agccgcaaaa gacggucaca cgacguauua       60

acaguacaau ucuuaauccu aggcaugcug cuaaugacag gggcaguuac ccgccgaggc      120

ucagcguacu acauguaccu ggaccguaac gacgccggcg aagcaauuag cuucccaacc      180

acacucggga ugaacaaaug cuacauccaa auaauggacc uaggacacau gugcgacgcc      240

acaaugucgu acgaaugucc aaugcuagac gaaggcgucg agccagacga cgucgacugc      300

uggugcaaua caaccagcac augggucgua uacggaaccu gccaccacaa gaaaggcgaa      360

gcccgccgau cccgccgcgc aguaacccuc cccagccaca gcacccgaaa acuccaaacc      420

cggagccaaa cauggcuaga gagcagggag uacacaaaac accugaucag gguagagaac      480

uggauauucc gaaaccccgg auucgcacuc gccgccgcag cuaucgccug gcuacucgga      540

uccuccaccu cucaaaaggu gaucuaucuu guaaugaucc uccucauagc acccgccuac      600

ucaauacgcu gcauaggagu uagcaacaga gacuucgucg aaggcaugag cggcggaaca      660

ugggucgaca ucguccucga gcacggcgga ugcgucacag ucauggccca ggacaaacca      720

accguagaca uagagcuagu aacaacaaca guauccaaua uggccgaagu ccgcuccuac      780

ugcuacgagg caucaaucuc agauauggcc ucagacucgc gauguccaac ccaaggggaa      840

gccuaccucg acaagcagag cgacacacag uacguaugua aaagaacccu uguugaccga      900

ggcuggggaa acggaugcgg ccuauuuggu aaaggaucac ucguaaccug ugccaaauuc      960

gcaugcucca agaaaaugac cggcaaaagc auacaacccg agaaccucga auaccgcaua     1020

augcucagcg uacacggcag ccaacacagc gggaugauag uaaacgacac cggacacgaa     1080

accgacgaaa aucgagcgaa aguagaaaua acaccaaaca gcccaagagc cgaagccacc     1140

cucggcggau ucggaagccu cggacuagac ugcgagccgc gcacagggcu cgacuucucc     1200

gaccucuacu accucacaau gaacaacaaa cacuggcucg uccacaaaga augguuccac     1260

gacauaccac uccccuggca cgccggcgcc gauaccggca ccccacacug gaacaacaaa     1320

gaggcccugg ucgaguucaa agacgcgcac gccaagcgcc aaacaguggu cguacucgga     1380

ucacaagaag gcgcaguaca cacagcccuc gccggagccc ucgaagccga aauggacggc     1440

gccaaaggac gccuaagcuc cggccaccuc aagugccgcc ucaaaaugga caaauugcga     1500

cucaagggag ucagcuacuc acugugcaca gccgccuuca cauucacaaa aauccccgcc     1560

gaaacacugc acggaacagu cacaguagaa guacaauacg caggcacuga cggaccaugu     1620

aaaguaccgg cgcaaauggc agucgacaug caaacccuca cgccagucgg acgacuaauc     1680

accgccaauc cagucaucac agaaagcaca gaaaacucaa aaaugaugcu agaacuagau     1740

cccccauucg gagacagcua caucgugaua ggcgucggcg aaaagaaaau cacccaccac     1800

uggcaccgaa gcggcucaac cauaggcaaa gcguucgaag ccaccgucag aggcgcuaaa     1860

cggauggcag uacucggcga cacggcaugg gacuucgguu cagucggggg cgcgcuaaac     1920

agccucggca aaggaaucca ccaaauuuuc ggggccgcau ucaaaagccu cuucggcgga     1980

augaguuggu ucucccaaau acuaaucggc acccuccuga uguggcuagg ccuaaacacc     2040

aaaaacgguu cgaucagccu caugugccua gcguugggag gcgugcuaau auuccuaagc     2100

acagccguaa gugccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  5052
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 1 opt

<400>  5052
gggagaaagc uuaccaugcg aauaauaaac gcaagaaagg aaaagaaacg gcgcggcgca       60

gacacauccg ucggaauagu gggacuccua cucacaaccg caauggccgc ggaaguaaca      120

agaagaggcu cagcguacua cauguaccua gaccgcaacg acgcaggaga agcaauaucc      180

uuccccacaa cccucggcau gaacaaaugc uacauacaga ucauggaccu cggacacaug      240

ugcgacgcaa ccaugagcua cgaaugccca augcuagaug aaggcgucga acccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguagucu auggaaccug ccaccacaag      360

aaaggcgaag cccgaagauc aaggagagca gucacacuac caagccacag cacacgaaaa      420

cuacaaacac guucacaaac cuggcucgaa uccagagaau acacaaaaca ccucauacga      480

gucgaaaacu ggauauucag aaaccccggc uucgcccucg cggcagccgc gauagcaugg      540

cugcugggaa gcaguaccuc acaaaaaguc auauaccuag uaaugauccu ccucaucgcg      600

cccgccuaca gcauucgcug cauaggugua agcaaccggg acuucguaga agggaugagc      660

ggcgguacau gggucgacau cguacuagaa cacgggggcu gcguuacagu aauggcacaa      720

gauaagccca ccguagacau agaacucguc acaacaaccg uaucaaacau ggcugagguc      780

agaagcuacu gcuacgaagc auccaucucc gacauggcaa gugacagucg cugccccacc      840

caaggagaag cguaccucga caaacaaucc gacacccaau acgucugcaa acgaacacuc      900

guagaccgcg gauggggaaa uggcugugga cuuuucggaa aaggcagccu aguaacaugc      960

gccaaguucg ccugcagcaa gaaaaugaca ggcaaaucaa uccaaccaga aaaucucgag     1020

uaccgcauua uguuaucagu acacggcagc caacacagcg gcaugaucgu caacgauacc     1080

gggcacgaaa ccgacgaaaa cagagccaaa guagaaauca caccaaacag cccaagagca     1140

gaagccacac uugggggcuu uggcagccua ggccucgacu gcgaaccacg aacaggccuc     1200

gacuucucag aucuauacua ccucaccaug aacaacaaac acuggcucgu acacaaagaa     1260

ugguuccacg acaucccccu ccccuggcau gccggggcag acacgggaac accccauugg     1320

aacaauaaag aagcccuagu agaauuuaaa gacgcccacg ccaaaagaca aaccgucguc     1380

guccucggca gccaagaagg ggccguccac accgcucuag ccggggcccu cgaagcagag     1440

auggacggag ccaagggacg gcuaagcucc ggccaccuaa aaugcagacu caagauggac     1500

aaacuaaggc ucaagggggu cucauacagc uuaugcacag ccgcauucac auucaccaaa     1560

aucccggcag agacauuaca cggcacaguc accgucgaag uacaauacgc aggcacagac     1620

ggcccaugca aagucccagc ccaaauggcg guagacaugc aaacacucac ccccgucggc     1680

cgccuuauca cagccaaccc agucaucacc gaaagcacgg aaaacagcaa gaugaugcuc     1740

gaacucgacc cacccuucgg cgacucguac auaguaaucg gagucggaga gaaaaagaua     1800

acccaucacu ggcaccgcag cggcuccacc auaggcaaag ccuucgaagc gaccguacgc     1860

ggcgcaaaac ggauggccgu acucggcgac acugccuggg acuucggauc aguaggcggc     1920

gcccucaacu cacuaggcaa gggaauccac caaaucuuug gcgcagccuu caaaagccua     1980

uucggcggaa ugucgugguu cagccaaauc cucauaggca cguuacucau guggcucggc     2040

cuaaacacca aaaacggcuc caucucccua augugccucg ccuuaggcgg aguccugauc     2100

uuccucagca cagcaguauc ggccgacgua ggaugcuccg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5053
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 1 opt

<400>  5053
gggagaaagc uuaccaugag aauaauaaac gcgcgcaaag aaaagaaacg acgcggcgca       60

gacacaagug ucggaaucgu cggccuccug cugacgacag ccauggcugc agaagucaca      120

cgcagaggaa gcgccuacua cauguaccua gaccgaaacg acgcaggcga agccauauca      180

uucccaacaa cgcucggcau gaacaaaugc uacauucaaa uaauggaccu agggcacaca      240

ugcgacgcaa cgaugagcua cgaaugcccc augcucgacg aaggagucga accagacgac      300

guagacugcu ggugcaacac gaccagcacg ugggugguau acggaaccug ccaccacaag      360

aaaggggaag cacgaagauc acgacgcgcc gugacccucc ccagucacag cacacgaaaa      420

cuccaaacac gcucccaaac auggcuagaa ucacgcgagu acacgaaaca ccucauccga      480

gucgaaaacu ggaucuuuag aaacccaggc uuugcacucg cagcggccgc cauugcaugg      540

cuacugggca guagcacauc ccagaaaguu auauaccucg uaaugauacu ucuaauagcg      600

cccgcauacu ccauccgcug cauaggagug uccaauagag acuucguaga aggaaugagc      660

ggaggcaccu ggguagacgu agucuuagaa cacggaggcu gcgucacagu cauggcccaa      720

gacaaaccga ccguagauau agaacucgua accaccacag ucagcaacau ggcagaagug      780

cgaucauacu gcuacgaagc cucaaucucc gacauggcca gcgacagccg gugcccaaca      840

caaggagaag ccuaccucga caaacaauca gacacccaau augucuguaa acgcacacuc      900

gucgacagag gguggggaaa cggcugcggc cucuucggca aagggucacu cgucacgugc      960

gcaaaauucg caugcagcaa gaaaaugacg ggcaaaucca uccaacccga aaaccucgaa     1020

uaccgaauaa ugcucagcgu ccacggguca caacacagcg gaaugauagu caaugacacc     1080

ggacacgaaa cagacgaaaa cagagcaaaa guagaaauca caccaaacuc accgcgagcc     1140

gaagccacac ugggaggauu cgggagucuc ggccucgacu gcgaaccccg gacuggccua     1200

gacuucuccg accuauacua ccuaacgaug aacaacaaac auuggcucgu ccacaaagaa     1260

ugguuccacg acaucccacu cccguggcac gccggcgccg acaccggcac uccccacugg     1320

aacaacaaag aagcccuugu cgaguucaaa gacgcccacg caaaacggca aacaguagua     1380

gucuuagggu cacaggaggg cgccguucac accgcccucg cuggggcgcu ugaggccgaa     1440

auggacggcg ccaaaggccg ccucuccagc ggacaccuga aaugccgacu uaaaauggac     1500

aaacuacgac uaaaaggagu cuccuacagc cucugcacag cagccuucac cuucacgaag     1560

aucccagccg aaacacuaca cggaacaguc acaguagaag uacaauacgc agggaccgac     1620

ggcccaugca aaguccccgc ccaaauggcc guagacaugc agacacuaac gcccguagga     1680

agacucauca cagcaaaccc gguaaucacc gaaagcaccg aaaacuccaa aaugaugcug     1740

gaacucgauc ccccauucgg agacagcuac auagucaucg gaguaggaga aaagaaaaua     1800

acacaccacu ggcaccgcag cggaucaacc auaggaaaag ccuuugaagc caccguaaga     1860

ggcgcaaaaa ggauggcggu ucugggggac acagcauggg acuucggcuc cguaggcggc     1920

gcacuaaacu cccucggcaa aggaauacac caaaucuucg gagcagccuu caaaucucuc     1980

uucggcggaa ugagcugguu cagccaaauc cuaauaggca cgcuacucau guggcuagga     2040

cuaaacgcca agaacggauc caucagccua augugccugg cucucggcgg cguguuaaua     2100

uuccuuagca cagcagucuc cgccgacgua ggcugcagcg uagacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5054
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 1 opt

<400>  5054
gggagaaagc uuaccaugcg caucauaaac gcgcgcaaag aacgcaaacg aagaggcgca       60

gauacaagca uaggaauaau cggacuccug cucacaacag cgauggcagc agagaucaca      120

cgaagggggu cagccuacua cauguaccuc gacagguccg acgcaggaaa agcaauauca      180

uucgcaacca cacuaggagu caacaaaugc cacguacaaa ucauggaccu aggacacaug      240

ugcgacgcca caaugucgua cgaaugcccg augcuggacg aaggagugga accggacgac      300

guagacugcu ggugcaacac aacaaguacg uggguggucu acggaacgug ccaccacaag      360

aaaggagaag cccgacgaag cagacgagca guaacacugc ccagccauuc aacaagaaaa      420

uugcaaacca gaucccagac auggcuggaa agcagggaau acaccaaaca ccucauaaag      480

guagaaaacu ggauauuccg aaaucccgga uucgcacucg uagccguagc aauagccugg      540

uuacugggcu caagcacaag ccaaaaagua auauaccuag ugaugauacu ccugaucgcc      600

cccgcguacu cgauccgaug cauaggaguc agcaaccggg acuuuguuga agggauguca      660

ggaggaacau ggguagacgu cgugcucgaa cacggaggcu gugugacagu aauggcgcaa      720

gacaaaccca cggucgacau agagcuagua acaacaacug uaagcaacau ggcagaagug      780

agaagcuacu gcuacgaagc cucgaucuca gacauggcaa gcgauagccg augcccuacg      840

caaggagaag ccuaccugga caaacaguca gacacccagu acgucugcaa acggacacug      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggga agggaucacu cgugaccugc      960

gcaaaguuca cgugcagcaa gaaaaugacc ggcaaaagca uacaaccaga aaaccuagaa     1020

uaccgaauca ugcucucagu acacggcucg caacacucag gaaugaucgg auacgaaaca     1080

gacgaagacc gagcaaaagu agaagucacu ccaaacagcc cgcgcgcaga agcaacacua     1140

gggggcuucg ggagccucgg gcuagacugu gaaccacgca cgggacugga cuucagcgac     1200

cucuauuacc uaacgaugaa caacaaacac uggcuggucc acaaagaaug guuccacgac     1260

aucccacuuc cguggcacgc cggagccgac accggcacac cccacuggaa caacaaagaa     1320

gcccuagucg aguucaagga cgcccacgcu aaaagacaaa ccgucgucgu ccucggcuca     1380

caagaaggag cgguccacac cgcgcucgcg ggcgcucucg aagcggagau ggacggugcc     1440

aaaggacgcu uauucuccgg gcaccuaaag ugcaggcuaa aaauggacaa auuaagacuc     1500

aaaggaguau ccuacucgcu cuguaccgca gccuuuaccu ucacaaaggu cccagccgaa     1560

acccuacacg ggacagugac aguugaaguc caguacgccg gcacagacgg accaugcaaa     1620

aucccgguac agauggcggu agacaugcaa acauugacac cagugggacg ccucauaacu     1680

gcuaacccag uaauaacaga aucaaccgaa aacaguaaaa ugaugcugga gcuggacccg     1740

ccauucggag acagcuacau cgugauagga guaggagaca agaaaauaac acaccacugg     1800

cacaggagcg gcagcaccau cggaaaagca uuugaagcga cagucagggg cgcgaaaaga     1860

auggcaguac uaggggacac cgcaugggac uuugggagcg ugggaggagu uuuuaacagc     1920

uuaggaaaag gcauccacca gaucuucgga gccgcauuca agagcuuguu cggaggcaug     1980

agcugguuca gccaaauacu aauagggacc cuccuggucu ggcuugggcu gaacaccaaa     2040

aacggaagca ucagccugac augccucgca cuaggaggcg uaaugauauu ccuaagcaca     2100

gccgugagcg cggacguggg gugcagcgug gacuucagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  5055
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 1 opt

<400>  5055
gggagaaagc uuaccaugcg aaucauaaac gcacggaaag aaaagaagag aagaggagca       60

gacaccagcg uagggaucgu gggccugcuc cucacaacgg ccauggccgc cgaagucacc      120

cgcagaggau cagcuuacua cauguaccua gaccgcaaug acgcgggcga agcaaucagc      180

uuccccacga cccuaggcau gaacaaaugc uacauacaaa uaauggacuu aggccacaug      240

ugcgacgcaa ccaugaguua cgaaugcccg augcuggacg aaggggucga accagacgac      300

guagacuguu gguguaacac cacaucaaca uggguggugu acgggaccug ccaccacaag      360

aaaggcgaag ccaggcgauc gagacgagcu guaacccuac caagccacag caccagaaaa      420

cuacaaacga gaucacaaac guggcuggag ucacgcgaau acaccaaaca ccucauccga      480

guugagaacu ggauauuccg aaacccgggu uucgcauugg cagcggccgc caucgccugg      540

uuacucggaa gcucuacgag ccaaaagguc aucuaccuag uaaugauccu ccucauagca      600

ccagcauaca gcauccgcug uauuggcguc agcaacaggg acuucguaga aggcaugagc      660

gggggcacau gggucgacgu aguacuugaa cacggcggcu gcgucaccgu aauggcacaa      720

gacaaaccca cagucgacau cgaacucgua acaacgaccg uaagcaacau ggcggaagug      780

cgaagcuacu gcuacgaagc aucaaucagc gacauggcga gcgacagcag augcccuacc      840

cagggcgaag cauaccucga caaacaaagc gacacacaau acguaugcaa gagaacucua      900

gucgaccgag gguggggcaa cggaugcgga cuauucggaa aaggcagccu cgucacaugu      960

gcaaaauucg ccugcucaaa gaaaaugaca gguaaaagca uacaacccga aaaccuggag     1020

uaccgcauaa ugcuaagcgu acacgggucc caacacagcg gcaugauagu caacgacacc     1080

ggacacgaga cagacgaaaa ccgagccaaa guggaaauaa ccccaaacag cccaagggca     1140

gaggccaccc uaggaggguu cggcagccuc ggccucgacu gcgaaccacg caccggccua     1200

gacuucucag accuauacua ccucacaaug aacaacaagc acuggcucgu acacaaagaa     1260

ugguuccacg acaucccccu uccauggcac gcgggagccg acaccggaac gccccacugg     1320

aacaacaagg aagcgcucgu ggaguucaaa gacgcccacg caaaacgaca aaccguggua     1380

guccuaggaa gccaagaagg ggcaguacac accgcacucg ccggagcacu agaagcggaa     1440

auggacggag cgaaagggag acucagcagc ggacaccuga aaugucgacu caaaauggac     1500

aagcuacggc ugaagggagu gagcuauucc cucugcacag cagccuucac auucacaaaa     1560

aucccggccg aaacacuaca cggaaccguc acuguggagg uacaauacgc cggcacagac     1620

ggccccugca agguaccagc ccaaauggca guagacaugc aaacacucac accagugggc     1680

cgccuaauaa cggcaaaucc aguuauaacc gagagcacgg aaaacuccaa gaugaugcuc     1740

gaacucgauc ccccguucgg agacucauac auaguaauag gcguagggga gaaaaagauc     1800

acccaccacu ggcacagauc cggcucgaca auaggaaaag ccuucgaagc caccguacgc     1860

ggagcaaaaa gaauggcggu acuaggagac acagcauggg acuucggauc cgugggcgga     1920

gcccuaaacu cccucggaaa gggaauacac cagauauucg gagcggcauu caaaucccua     1980

uucggaggaa ugagcugguu cagccagauc cucauaggaa cacuacuaau guggcuaggu     2040

cucaacacca aaaacggaag cauuagccuc augugccucg cacuaggcgg uguacuaauu     2100

uuccuaagca cagccguauc agcggacgua ggaugcagcg uagacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5056
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 1 opt

<400>  5056
gggagaaagc uuaccaugcg gaucauaaac gccaggaaag aacgaaaacg gagaggagca       60

gacacgagca uuggcaucau cgggcuacua cuaacgaccg cgauggcggc ggagaucaca      120

cgaagaggaa gcgcauacua cauguaccug gacagguccg acgccggaaa agccauuuca      180

uuugccacaa cacuaggggu aaauaagugc cacguacaaa uaauggaucu uggacacaug      240

ugcgacgcaa caauguccua cgaaugccca augcuggaug agggcguaga accagacgac      300

gucgacugcu ggugcaacac gacaucgacg ugggucguau acggaacaug ccaccacaag      360

aagggggaag cacgaagauc gcggagagca guaacacucc cgagucacuc gacacggaag      420

cuacaaacaa ggagccaaac auggcuggag uccagagaau acacaaagca ccuaauaaaa      480

gucgaaaacu ggaucuuccg caaccccggu uuugcccuag ucgcagucgc aauagccugg      540

cuacuaggcu caagcaccag ccagaaagua auauaucuag ugaugauacu ccugauagcg      600

ccagcguaca gcauuaggug cauaggagua agcaaccgcg acuucguaga aggcaugucg      660

ggcgggaccu gggucgacgu cguauuagaa cacggaggcu gcgucaccgu aauggcacag      720

gacaaaccaa caguagacau cgaacucgua accaccacag ucagcaacau ggcggagguc      780

agaagcuacu guuacgaggc aucuauaagu gacauggcca gugacagccg cuguccaaca      840

caaggagaag cauaccuaga caaacaauca gacacacaau acgucugcaa gagaacccua      900

guagaccggg gaugggggaa cgggugcgga cuuuucggca agggcucgcu cguaacaugc      960

gcaaaauuca cgugcucgaa gaaaaugacg ggcaaaagca uccaaccaga aaaccucgaa     1020

uacaggauaa ugcuaucggu gcacggcuca caacacucag gaaugauagu aaacgacacc     1080

ggauaugaaa cagacgaaaa ccgggcaaag guagagguaa caccgaacag cccccgggca     1140

gaggcgacac uaggcggcuu cggaagccua ggcuuagacu gcgaaccaag aacaggccuc     1200

gacuucucag accucuacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagag     1260

ugguuccacg acauaccgcu cccauggcac gcgggagccg acacaggaac accgcacugg     1320

aacaacaaag aggcccuagu agaguucaaa gacgcacacg caaaaagaca aacaguagua     1380

gugcuaggca gccaagaagg cgccgugcac acagcccucg ccggagcgcu ggaagccgaa     1440

auggacggcg cgaaaggcaa acuguucucc ggacaccuaa agugccggcu caaaauggac     1500

aagcugcgac ucaagggcgu aagcuacucc cuguguacag cagccuucac auucacaaaa     1560

gugccggccg agacccuaca cgggaccgug acaguagaag ugcaauacgc cggaacagac     1620

ggccccugca agaucccggu acaaauggca gucgauaugc aaacacuaac acccgucggg     1680

agauugauaa ccgcaaaccc cguaauaaca gaaaguacag agaacaguaa aaugaugcua     1740

gaacuggacc cgcccuucgg cgacagcuac auagucaucg gcgucggaga uaagaaaauc     1800

acacaccacu ggcaccgaag cgggagcaca auagggaaag cguucgaggc aacagucagg     1860

ggagcgaaac ggauggccgu acuaggcgau accgcguggg acuucggaag ugucggcggc     1920

guauucaacu cacuagggaa aggaauccac caaaucuucg gagcagccuu caagagccua     1980

uucggcggca ugaguugguu cucacaaaua cugauaggca cacugcuggu auggcuaggg     2040

cuaaauacca aaaacgggag cauaagccua accugccucg cacucggugg cgugaugauc     2100

uuccugagca cagccguaag cgcggacgua gggugcagcg uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5057
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 1 opt

<400>  5057
gggagaaagc uuaccaugag aaucaucaac gccaggaaag agcgaaaacg ucguggagca       60

gacacaucga uaggcauagu cggccuacug cucacaaccg caauggccgc agagauaaca      120

cgcagaggcu cagcauacua cauguaccuc gaccgaagug acgccggaaa agcaaucuca      180

uucgcaacaa cacuaggcgu aaacaaaugc cacguacaaa uuauggaccu aggucacaug      240

ugcgacgcca ccaugucaua cgagugcccg augcuagacg aaggcgugga accagacgac      300

guagacugcu ggugcaacac aacgagcacc ugggucguau acgggacgug ccaccacaag      360

aaaggcgaag caagacgaag ccggcgcgcc gugacacucc caagccacag cacccgaaaa      420

cuacagacca gaagccagac uuggcuagaa agccgagaau acaccaaaca ccucaucaaa      480

guagaaaacu ggaucuucag aaacccaggg uucacgcucg uagccguagc cauagccugg      540

cucuuaggcu caagcacguc gcaaaaaguc aucuaccuag ugaugauacu ccugauagcg      600

cccgccuaca gcauacgaug caucggaguc agcaaccgcg acuucgugga gggcauguca      660

ggagggaccu gggucgacgu aguccuggaa cacggaggcu guguaacagu aauggcccag      720

gacaaaccaa cagucgacau cgaacucgua accaccaccg uguccaacau ggcagaaguc      780

agaucuuacu gcuacgaagc cagcaucuca gacauggccu cagacucacg cugucccaca      840

caaggagaag cguacuuaga caaacaauca gacacccaau acguaugcaa acgcacgcuc      900

gucgaucgag guuggggaaa cggcugcgga cuauucggca aaggcucgcu cguaaccugu      960

gcaaaauuca caugcagcaa gaaaaugaca ggcaaaagca uccaaccgga aaaccuagaa     1020

uaccggauca ugcucucggu acacggcucc caacacagcg gaaugauagu caacgacgag     1080

aaccgcgcaa aaguagaagu caccccaaac ucuccccgcg cagaagcaac ccuaggaggg     1140

uucggauccc uaggcuugga cugcgaaccc agaacaggac ucgacuucag cgaccucuac     1200

uaccuaacaa ugaacaacaa gcacugguug guucacaaag aaugguucca cgacaucccu     1260

cucccauggc augcgggggc agacacaggg acgccucacu ggaacaacaa agaagcccua     1320

gucgaguuca aagacgcgca cgcaaaacgc caaacggucg uaguacucgg cagccaagaa     1380

ggcgcggucc acaccgcgcu agccggcgcu cucgaagcgg aaauggacgg agcaaaagga     1440

cgccuauucu ccggccaccu caagugccga cuaaagaugg acaaacuacg auugaaaggg     1500

guauccuauu cccucugcac agcagcauuc accuucacca agguccccgc cgaaacccua     1560

cacggcaccg ucaccgucga agugcaauac gcaggaaccg acggcccgug caaagucccc     1620

gcacaaaugg ccgucgauau gcagacacua acaccgguag gacgacugau cacugcgaac     1680

cccgugauca ccgaaagcac cgaaaacuca aaaaugaugc ucgaacuaga cccuccguuc     1740

ggcgacagcu acaucgucau cggggucggc gacaagaaaa uaacccacca cuggcaccgc     1800

agcggaucaa cuaucggcaa agcguucgag gccaccguaa gaggcgccaa acggauggca     1860

guacuaggcg auacagcaug ggauuucggg uccguaggag gcgucuucaa cagccucgga     1920

aaaggcaucc accaaauauu cggcgccgcg uucaagagcc uauuuggcgg aaugagcugg     1980

uucucacaaa uccugaucgg aacucuccua gucuggcuag gacuaaacac gaaaaaugga     2040

ucaauaagcc uaacaugccu ggcacuagga ggcgucauga uauuccuaag uacagcaguc     2100

agcgccgacg ucggaugcag cguagacuuc agcaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  5058
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 1 opt

<400>  5058
gggagaaagc uuaccaugcg caucauaaac gcgagaaaag aacgaaaacg ccgcggggcg       60

gacacgagca ucggcauaau cggacuguug cuaacgaccg cgauggccgc ggagaucaca      120

agacgaggca gcgcguacua uauguaccuc gaccgguccg acgccggaaa agccaucucg      180

uucgccacca cccuaggagu caacaaaugc cacguccaaa uaauggaccu cggccacaug      240

ugcgaugcca caaugucaua cgagugcccc augcuagacg aagguguaga accagacgac      300

guagacugcu ggugcaauac cacgagcacg uggguaguuu acggcacgug ucacagaaag      360

aaaggagaag ccaggagauc aagacgcgcg gucacccucc caagccacuc aacacgcaaa      420

uuacagacca gaagccagac cuggcucgag uccagagaau acaccaaaca ccugauaaaa      480

gucgaaaacu ggaucuuucg caaccccgga uucgcacucg uggcaguagc gauagcaugg      540

cuccuaggca gcagcacauc ccaaaaagug auuuaucucg ucaugauccu acugauagca      600

ccggcguaca gcaucaggug cauaggagua aguaacaggg auuuuguaga aggcaugucu      660

ggcggaacgu ggguagacgu cguacucgag cacggaggcu gugucacagu cauggcccaa      720

gacaaaccca cagucgacau cgagcucguc accacgacag ucagcaacau ggcagaagug      780

cgaucauacu gcuacgaagc gucgaucagc gacauggcau ccgacucgcg cugccccacg      840

caaggugaag cauaccuaga caagcaauca gacacacaau augucugcaa acgcacccuc      900

gucgaccgag gauggggcaa cggaugcgga cucuuuggaa aaggaucccu aguaaccugc      960

gccaaauuua ccugcagcaa aaagaugacg ggcaagagca uccaacccga aaaccucgag     1020

uacagaauca ugcuguccgu ccacggcucg caacacucag gaaugauagu caacgacaca     1080

ggcuacgaaa cagaugaaaa cagggcaaaa gucgaaguca cccccaacuc cccacgcgcg     1140

gaagccaccc ugggcggauu cggcucccua ggccuagacu gcgaaccacg cacaggacuc     1200

gacuucagcg accuguacua ccuaacaaug aacaacaaac acuggcuagu ccacaaggag     1260

ugguuccacg acauaccacu ccccuggcac gcaggcgccg acaccggaac cccgcacugg     1320

aacaacaaag aagcacucgu cgaguucaaa gacgcacacg caaagagaca aacgguagua     1380

guccugggau cccaagaagg cgcaguacac acagcacugg ccggcgcccu agaagccgaa     1440

auggacggcg cgaaaggaaa acuguucagc ggccaccuca aaugccgccu aaaaauggac     1500

aaacuacgac ucaaaggagu aucuuacucc cuauguaccg cagccuucac cuucaccaaa     1560

guaccggccg aaacgcucca cggaacugug acagucgagg uacaauacgc cggcaccgac     1620

ggacccugca aaauaccagu acaaauggcc guagacaugc aaacgcuaac gcccguagga     1680

cggcuaauca cagcgaaccc cgugauaaca gaaucaaccg agaacucaaa aaugaugcuc     1740

gaacuagacc cgcccuucgg cgacagcuau auagugauag gcguaggcga caagaaaaua     1800

acccaccacu ggcacagaag cggauccacc aucggcaaag cguucgaagc aaccguaagg     1860

ggcgccaaaa gaauggcagu ccuaggagau accgccuggg acuucggaag cgucggagga     1920

gucuucaaca gccuuggaaa agggauucac caaaucuucg gagccgccuu caaaagccua     1980

uucgggggca uguccugguu cagccaaaua cuaaucggca cccuauuagu cuggcucggc     2040

cucaauacca aaaauggcag cauuagccua accugccuag cacucggagg cgucaugauc     2100

uuccugagca cagccguaag cgccgacgua ggcugcuccg uagacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5059
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 1 opt

<400>  5059
gggagaaagc uuaccaugcg cauaaucaac gccagaaaag aacggaaacg acgaggcgcg       60

gacacaucaa uaggaaucau aggacugcug cugacaacag cgauggcggc cgagauaaca      120

agacgaggcu cggccuacua cauguaccuc gaccggagcg acgcagggaa agcgauauca      180

uucgcaacga cacucggagu aaacaagugc cacguacaaa ucauggaccu cggacacaug      240

ugcgacgcca cgaugucaua cgagugcccc augcuggacg agggcgucga accagacgac      300

guagacugcu ggugcaacac cacaagcaca uggguaguau acggaaccug ccaccacaag      360

aaaggcgaag cgcgaagguc aagacgagca gucacccuac caagccacag cacaagaaaa      420

cuccaaaccc gcucacaaac cuggcuagaa agccgagaau auaccaaaca ccucaucaaa      480

guagaaaacu ggauauuccg caacccaggc uucgcacucg uggcaguagc cauagcaugg      540

uuauuaggca guagcacuag ccaaaaggua aucuaccuag ucaugauacu ccuaaucgcg      600

cccgcguacu caaucagaug caucggagua agcaacagag acuucguaga agggauguca      660

gggggaacau ggguagacgu cguacuagaa cacggaggau gcgucacggu aauggcccag      720

gacaagccaa ccgucgacau agagcucgug accacuaccg uaagcaacau ggcggaagua      780

cgaucauauu gcuacgaagc cucaauauca gacauggccu cugacagccg cugcccaaca      840

caaggagaag cguaccuaga caagcaaagc gacacgcaau acgucugcaa acggacacuc      900

guagaccgcg gauggggaaa cggcugcggu cuauucggaa agggcucgcu cguaaccugc      960

gcaaaauuua ccugcucaaa gaaaaugaca ggcaaaucaa uucagccgga aaaccucgag     1020

uacaggauca ugcucuccgu acacggcuca caacauagcg gcaugacagu caacgacaua     1080

ggauacgaaa cugacgaaaa cagggccaaa gucgaaguaa cgcccaacag cccaagagca     1140

gaagccacac ugggaggauu cggcagcuua ggccuggacu gcgaaccgcg aaccggauug     1200

gacuucucag accucuacua ccuaaccaug aacaacaaac acuggcuagu ccacaaagaa     1260

ugguuccacg acauaccacu accguggcac gcaggcgcag acaccgggac cccgcacugg     1320

aacaacaaag aagcgcuggu cgaguucaaa gacgcgcaug ccaaacgaca aaccgucgug     1380

gucuuaggau cccaagaagg cgcgguccac acggcacugg caggcgcucu cgaagccgaa     1440

auggacggag caaaaggaaa gcuauucagc ggccaccuca aaugcagacu caaaauggau     1500

aaacugaggc ucaaaggggu aucguacucc cugugcacag cagccuucac cuucacaaaa     1560

guccccgcag agacccucca cggcacagug acaguggaag uccaauacgc cgggacagac     1620

ggaccaugca aaaucccagu ccagauggca guagauaugc aaacacugac acccguagga     1680

agacuaauca cggcaaaccc cgugauaaca gaaucgacag aaaacuccaa aaugaugcug     1740

gaauuagacc cacccuucgg cgauagcuac aucguaauag gagucgggga caagaaaaua     1800

acacaccacu ggcaccgcag cggaagcacc aucggcaaag cauucgaggc gacggucaga     1860

ggcgcaaaac gaauggcggu cuuaggagac acagcauggg acuucggguc cgugggaggg     1920

guguucaacu cacuaggaaa aggaauccau caaaucuucg gagccgcauu caaaagccua     1980

uucggaggga uguccugguu cucacaaaua cuaauaggca cacugcuggu auggcuagga     2040

cucaacacca aaaacgggag cauaagccua accugccucg cacuaggggg aguuaugauc     2100

uucuugagca cggccgucag cgcagacguc ggcugcucag uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5060
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 1 opt

<400>  5060
gggagaaagc uuaccaugcg cauaaucaac gcccgcaaag aacgcaaacg aagaggcgca       60

gacacaagca uaggcaucau cggccuccuc cucacaaccg ccauggcagc agaaauaacc      120

cgcagaggaa gcgccuacua cauguaccua gaccgcuccg acgcaggcaa agccauauca      180

uucgcaacaa cccugggagu aaacaaaugc cacguccaaa uaauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgaaugccca auguuagacg aaggaguaga acccgacgac      300

gucgacugcu gguguaacac aacuagcaca uggguagucu auggaaccug ccaccacaag      360

aaaggagaag caagacgauc ccgccgcgcg gugacccugc caagccacuc aacacgaaaa      420

cuacaaacca gaucccaaac cuggcucgaa ucacgugaau acaccaaaca ccucauaaag      480

gucgagaauu ggauauuccg aaacccaggc uucgcccuag uagcaguagc aauagcaugg      540

cugcucggga guagcaccag ccaaaagguc aucuaccuag ucaugauacu acucauagcc      600

ccagcauaca gcauccgaug cauaggagua uccaaccgcg acuucguaga agggaugagc      660

ggaggcaccu ggguagacgu aguccucgaa cauggaggau gcguaacggu aauggcacag      720

gacaagccca cggucgacau agaauuagug acgaccacag uauccaacau ggccgaggua      780

cgaaguuacu gcuacgaagc gucaauaucc gacauggcga gugacucgcg cugccccaca      840

caaggggagg cuuaccucga caaacagagc gacacacaau acgucugcaa acgaacccua      900

gucgaccgcg gaugggguaa cggcugcgga cuauuuggca aaggcagccu agugaccugc      960

gccaaauuca cauguuccaa aaagaugacu ggaaaaucca uacaaccaga aaaccucgaa     1020

uaccgcauca ugcuauccgu acacggcuca caacauucag gcaugauagg guacgaaaca     1080

gacgaaaacc gcgccaaagu agaagucaca ccaaacagcc caagagcaga ggccacacua     1140

ggaggcuucg gaucccucgg acucgacugc gagccgcgaa ccggccucga cuucucggac     1200

cuauacuacc ucacaaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac     1260

aucccccugc cauggcacgc aggcgcagac accggaacac cgcacuggaa caacaaagaa     1320

gcacugguag aguucaaaga cgcccaugcc aaacgccaaa caguaguagu ucugggaucc     1380

caagaaggcg caguccacac ugcccucgcg ggcgcccucg aagcagaaau ggacggcgcu     1440

aaaggcaaac uauucuccgg gcaccucaaa ugucgacuaa aaauggacaa acuccgacua     1500

aaaggcguaa gcuacucucu cugcaccgcc gcguucacgu ucacaaaagu ccccgccgaa     1560

acacuccacg gcacagucac cgucgaaguc caauacgccg gaaccgacgg acccugcaaa     1620

auacccgugc agauggcagu agacaugcaa accuuaacac cagucggacg ccucauaacc     1680

gcaaacccag ucaucacaga aagcaccgaa aacuccaaaa ugaugcuaga acucgacccg     1740

cccuucggcg acuccuacau cgugauaggc gugggggaca agaaaaucac acaccacugg     1800

caccgaagcg gcagcacaau cggaaaagcc uucgaggcca cagucagagg cgcaaaaaga     1860

auggccgugc uaggagacac cgcaugggac uucgguuccg uaggcggcgu auucaacagc     1920

cucggcaagg gaauccacca aaucuucggc gcggcuuuca aaagccucuu cggcggcaug     1980

uccugguucu cacagauccu gaucggcaca cuccucgucu ggcuaggccu aaacaccaaa     2040

aacggcucga uaaguuuaac augccucgca cugggcggag ucaugaucuu ccucucaacc     2100

gcaguuagcg ccgacguagg augcagcgua gauuucucca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  5061
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 1 opt

<400>  5061
gggagaaagc uuaccaugag aaucaucaac gcacgaaagg aaaagaaacg cagaggagca       60

gacacaagcg ucggcauagu aggacuccua cuaacaacag ccauggccgc agaagucaca      120

agaaggggaa gcgcauauua cauguaccuc gaccgcaacg acgcuggcga agccaucagc      180

uucccgacca cccuaggcau gaacaaaugc uauauacaaa uaauggaccu cggccauaug      240

ugcgacgcaa caaugagcua cgaaugcccc augcucgacg aaggcgucga accagacgac      300

gucgacugcu ggugcaacac aaccucaaca ugggucgucu acggcacgug ccaccacaaa      360

aagggagaag ccagacgcuc ccgacgagcc guuacccucc cuagccacag cacgagaaaa      420

cuccaaacac gaucacaaac cuggcuugaa agccgcgaau acaccaaaca ccucauccgc      480

guggaaaacu ggauauuccg aaaucccggc uucgcccucg cagcagccgc gaucgcaugg      540

cuccuuggcu ccuccacguc ccagaaaguc aucuaucucg ucaugauccu acuuaucgcg      600

ccugcauacu ccauaagaug caucggcgua agcaacagag acuucgucga aggcaugucc      660

ggcggcaccu gggucgacgu cguccuagaa cacggggguu gcguaaccgu cauggcucag      720

gacaaaccaa cagucgacau cgaacuggua acaacgaccg ucagcaacau ggccgaagua      780

cgcagcuacu gcuaugaggc uucaauaagc gacauggcca gcgacagccg gugcccaaca      840

caaggagaag ccuaccuaga caaacaauca gacacccaau acgucuguaa gagaacccug      900

guggaccgcg gauggggaaa cggcugcgga uuguucggca agggcucccu aguaacaugc      960

gcaaaauucg ccugcuccaa gaaaaugacc ggcaaaucga uccaacccga aaaccucgaa     1020

uaccgcauaa ugcuauccgu ccacggcuca caacacucag gcaugauagu aaacgacacc     1080

ggacacgaaa cagacgaaaa uagagcgaaa guggagauca cucccaacag cccaagagcg     1140

gaagccacac uaggaggcuu cggcucccua ggacucgacu gugaaccacg aaccggacuc     1200

gauuucucgg accucuacua ccuaacaaug aacaacaaac acuggcucgu gcacaaagaa     1260

ugguuccacg acauaccccu cccuuggcac gcaggagcag acaccggcac cccacacugg     1320

aacaacaaag aagcccucgu agaguucaaa gacgcccacg ccaaaagaca aacaguugua     1380

guacugggca gccaagaagg agccgugcac accgcccuag ccggagcccu agaagccgaa     1440

auggacgggg cuaaaggccg uuuauccagc ggccaccuaa aaugcagacu aaaaauggac     1500

aaacuaaggu uaaaaggcgu aagcuacucc cuaugcacgg cggcauucac cuucacaaaa     1560

auccccgcag aaacccucca cggaacaguc acaguggaag uucaauacgc cggcacagac     1620

ggacccugua agguaccagc acaaauggcu gucgacaugc agacucugac accaguuggg     1680

cgacucauca ccgcaaaccc cgucaucacu gaaagcaccg aaaacuccaa gaugaugcua     1740

gaacuugacc caccauucgg agacagcuac auaguaauug gcgucggcga aaagaaaauc     1800

acacaccacu ggcacagaag cggcagcacg auagguaaag cauucgaagc aaccguaagg     1860

ggcgccaaac gaauggcagu gcucggagac accgccuggg acuucggaag cguaggaggc     1920

gcccugaacu cacuaggccg aggcauacau caaauauucg gggcagcauu caaaagccuc     1980

uucggcggca ugagcugguu cagucaaaua cuaauaggga cacugcuaau guggcuagga     2040

cugaacacca aaaacggcuc aauaucacua augugccuag cacucggagg cgugcuaaua     2100

uuccuaagca cagcagucuc ggcagacgua ggaugcagcg uagacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5062
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 1 opt

<400>  5062
gggagaaagc uuaccaugcg caucaucaac gcaaggaagg agaaaaagcg acgaggcgca       60

gacgcgagcg ucggcauagu aggccuccug cucacaaccg cgauggccgc cgaggucaca      120

cgcagaggcu cggccuauua cauguaccuc gacagaaaug acgcaggcga agccaucuca      180

uucccgacca cacucggaau gaacaaaugu uacauacaaa ucauggaccu agggcacaug      240

ugcgacgcaa cuauguccua cgaaugcccg augcucgacg aaggaguaga accagacgac      300

guagauugcu ggugcaacac aacaagcaca uggguagucu auggcacgug ccaccacaaa      360

aagggggagg cgaggaggag cagaagggca guuacacucc caagccacag cacgcgcaaa      420

cuacaaacaa ggagccagac cuggcuugaa agcagagaau acacaaagca ccugaucagg      480

gucgaaaacu ggauauuccg caacccgggu uucgcacuag cagcagcagc aaucgcaugg      540

cuccuaggaa gcagcacguc ccaaaaagua auauaccucg uaaugauacu gcucaucgcc      600

ccagcuuauu caaucaggug caucggcgug agcaacagag acuuugugga aggaauguca      660

ggcggcacgu gggucgacgu cguucuagaa cacggaggau gcgucacagu aauggcucag      720

gacaaaccaa cagucgacau agagcuagua acaacaaccg uaagcaacau ggcagaaguu      780

cgcaguuacu gcuaugaagc cucgaucuca gacauggccu cagacucgag augccccaca      840

caaggagaag cguaccuaga caaacaauca gacacccaau acguaugcaa aaggacccua      900

guagaccgcg gaugggggaa cgggugcgga cuauucggaa agggaucgcu cguaaccugc      960

gcaaaauucg ccugcagcaa aaagaugacu ggcaaaagca uacaacccga aaaccuggaa     1020

uacagaauca ugcuaucagu ccacgggagc caacacucag gcaugauagu aaacgacacg     1080

ggccacgaaa cagacgaaaa cagagcuaaa guagagauaa cacccaacag cccacgagcg     1140

gaagcgacac uaggagguuu cggaagccua ggccuggacu gcgaaccgcg aacaggccuc     1200

gauuucagcg aucucuacua ccugaccaug aauaacaaac acuggcucgu acauaaagag     1260

ugguuccacg acauaccgcu accauggcau gccggagcag acaccggaac accccacugg     1320

aacaacaaag aggcccuagu cgaguucaaa gacgcgcacg ccaaaagaca aacaguggug     1380

guacugggau cgcaggaggg cgcgguccac acugcacucg ccggcgcgcu agaagccgag     1440

auggacggag caaaaggacg ccuaagcagc ggacaccuca agugccgacu aaagauggac     1500

aagcuccgcc uaaaaggggu cucguacagu cucugcacgg cagcauucac cuucacgaag     1560

aucccggcgg aaacacugca cggaaccgua acaguagaag ugcaauacgc cgggaccgac     1620

ggcccgugca aagucccggc ccaaauggca guagacaugc aaacgcuaac accaguagga     1680

agacugauca cugccaaccc aguaaucaca gaaucgacag agaacucaaa aaugaugcua     1740

gaauuggacc cgcccuuugg cgacagcuac aucgugauag gaguagggga aaagaaaaua     1800

acacaccacu ggcaccgaag cggaagcaca aucgggaaag ccuucgaggc aacaguccga     1860

ggcgcaaaga gaauggcagu ccuuggagac acggcauggg acuucggaag cgucgggggc     1920

gccuugaacu cacuaggcaa agggauccac caaauauucg gagccgcguu caagagccuc     1980

uucggcggca uguccugguu uucacaaaua cucaucggaa cauuacugau guggcucggg     2040

cuuaacacca agaacggcuc aauaagccug augugccucg cccuaggggg cguacucaua     2100

uuccugucca cggccgucag cgccgacgua ggaugcagcg uagauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5063
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 1 opt

<400>  5063
gggagaaagc uuaccaugcg aaucaucaau gcacggaaag agaaaaagcg ccgaggggcc       60

gacacgagcg ucgggaucgu aggccuacug cugaccaccg caauggccgc agaagucaca      120

cgacgaggca gcgcauacua cauguaccuc gacagaaacg acgccggaga agccaucuca      180

uuccccacaa cacucgggau gaacaaaugc uacauccaaa uaauggaccu aggacacaug      240

ugcgaugcaa ccaugagcua cgaaugccca augcucgaug aaggcguuga accagacgac      300

guggacugcu ggugcaacac aacuagcacc ugggucgucu acggcacaug ccaccacaag      360

aaaggggagg cacgcagaag ucgacgggcc guaaccuuac ccagccacuc cacaagaaaa      420

cuacaaacac gcucacaaac cuggcuggaa agucgagaau acaccaaaca ccucauccga      480

guggaaaacu ggaucuucag aaacccggga cuagcacuag ccgccgcagc aaucgcaugg      540

cuacuaggaa gcuccacaag ccaaaaaguc auauaccucg ucaugauccu acugaucgcc      600

ccagcauaca guauacgcug cauaggagug agcaacagag acuucguaga aggcauguca      660

ggaggaacgu gggucgacgu aguccucgag cacggcggcu gcguaaccgu aauggcacaa      720

gacaaaccca ccguagacau agaacucguc accacaaccg uuagcaacau ggcagagguc      780

aggucuuacu gcuacgaagc aagcauaagc gacauggcau ccgacucaag augccccacc      840

caaggcgaag cauaccuaga caaacaaucc gacacacaau acguaugcaa acgaacccua      900

guagaccggg gcuggggcaa cggcugcgga cuauucggca aagggagccu agucacaugc      960

gcaaaauucg cauguuccaa gaaaaugacc ggcaaaucca uccaaccaga gaaccucgag     1020

uaccgcauaa ugcugucagu acacggcucc caacacuccg gaaugauagu gaacgacacc     1080

ggccacgaaa ccgacgagaa cagagccaaa gucgaaauaa cacccaacuc accccgggca     1140

gaggcaaccc ucggaggcuu cggcagucuc ggacucgacu gcgaaccccg uaccggucuc     1200

gacuuuucag accuauacua ccuuacuaug aacaacaaac acuggcuagu gcacaaagag     1260

ugguuccacg auaucccccu accauggcac gccggggccg acaccggcac accacacugg     1320

aacaacaaag aagcgcucgu cgaguucaaa gacgcgcacg caaagcgcca aaccguggua     1380

guccuaggca gccaagaagg ggcaguccac acagcacuag ccggagcgcu ggaagcagaa     1440

auggacggag ccaaaggccg ccuauccagc ggacaucuaa aaugcagacu aaaaauggac     1500

aaacuccguc ucaaaggagu auccuacagc cucugcacag cagcuuucac cuucacgaag     1560

auccccgccg aaacccucca cggcacaguc acagucgaag uccaguaugc cggaacagac     1620

ggaccaugca aagugccggc ccaaauggcc guggacaugc aaacacuuac cccaguaggc     1680

cggcuaauca cagcaaaccc agugaucacc gaaagcaccg aaaacuccaa aaugauguua     1740

gaacuggacc cacccuucgg cgauuccuac aucgucaucg gcgugggcga aaagaaaauc     1800

acacaccauu ggcaccgaag cggcuccacc aucggaaagg cauucgaagc caccguaaga     1860

ggcgccaagc gcauggcagu acuaggagac acagcguggg acuucggcag cgucggagga     1920

gcucuaaaca gccugggaaa ggguauacac caaaucuucg gagcggccuu caaaagccuc     1980

uucgggggca ugucaugguu cagccagauc cuaaucggaa cccuacuaau guggcucggc     2040

cuaaacacca agaacggaag cauaucgcug augugcuuag cacucggcgg aguacuuauc     2100

uuccucucaa cagccguauc cgccgacguc ggcugcagcg ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5064
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 1 opt

<400>  5064
gggagaaagc uuaccaugcg gaucaucaac gcacgcaaag agaagaaacg acggggagcc       60

gacaccucag uaggcaucgu gggacuacuu cucacaacgg caauggcagc cgaaguaaca      120

cgcagaggaa gcgccuacua cauguaccua gaccgcaacg acgccggcga agcaaucagc      180

uuccccacaa cccuaggaau gaacaaaugc uacauacaaa uaauggaccu cggccacaug      240

ugcgacgcca caaugucaua cgaaugcccc augcuagacg aaggaguaga acccgacgac      300

gucgacugcu ggugcaacac gacaagcacc uggguuguau acggaaccug ccaccacaag      360

aaaggcgaag cacgccgcuc acggagagcc guaacccucc ccucacacag cacaagaaaa      420

cugcaaacac ggucgcaaac cuggcuagaa ucacgcgaau acaccaaaca cuuaauccga      480

gucgaaaacu ggauauuccg caauccaggc uucgcacucg ccgcagccgc caucgcaugg      540

cuacugggaa gcuccacaag ccaaaaaguu aucuaccucg uaaugauccu acuaaucgcc      600

cccgcuuacu cgauacggug cauaggagua ucgaacaggg acuucguaga gggcaugucc      660

ggcggcacau gggucgacgu cgucuuagag cacgggggcu gcguaaccgu cauggcucaa      720

gacaaaccga ccguggacau cgaacucgua accacaaccg ugagcaacau ggccgaagua      780

agaagcuauu guuacgaagc aagcaucuca gacauggcaa gcgacagcag augcccaacc      840

cagggagaag cauaccuaga caaacaauca gauacacaau acgugugcaa aaggacacuc      900

guagacagag gaugggggaa cggaugcggg cuuuucggaa aaggaucacu cgucacaugc      960

gcaaaauucg caugcagcaa gaaaaugaca ggcaaaucaa uccaacccga gaaccuggaa     1020

uacagaauaa ugcuauccgu ccacgggagc caacacuccg gaaugauagu aaacgacaca     1080

ggacacgaaa cugacgagaa ccgagccaaa gucgagauca caccaaacag cccacgcgcg     1140

gaagccacac ucggcggauu cggaucccuc ggccucgacu gcgagccacg aaccggccua     1200

gacuucucgg accuauacua ccuaaccaug aacaacaaac acuggcuggu gcacaaagaa     1260

ugguuccaug acauaccgcu uccauggcac gcaggagcag acaccggcac accccacugg     1320

aacaacaaag aagcgcuugu ugaguucaaa gacgcccacg ccaaaagaca aaccguagua     1380

guacucggca gccaagaagg ugccguacac acagcgcuug ccggcgcccu cgaagcagaa     1440

auggacggcg caaaaggacg ccuuagcagc ggccaccuaa aauguaggcu caaaauggac     1500

aaacuaagac uaaaaggggu cuccuauucc cuaugcacgg cagcauucac cuucacgaaa     1560

auccccgcag aaacccucca cggaaccgua acagucgaag uacaguacgc aggaaccgac     1620

ggaccgugca aagucccagc ccagauggca guggacaugc aaacccugac gccagucggc     1680

cgacucauca ccgccaaccc ugugaucacg gagagcacag aaaacagcaa aaugaugcuc     1740

gaacucgacc cacccuucgg cgacucguac auagucauag gcguaggcga aaagaaaaua     1800

acacaccacu gguaccgcuc cggcagcaca aucgggaaag cauucgaagc cacagucaga     1860

ggggcgaaac gcauggccgu acuaggagac acagccuggg acuucggcag cguaggaggc     1920

gcacuaaaca gccuagggaa aggcauacac caaaucuucg gagccgccuu caaaagccua     1980

uucgguggaa uguccugguu cucccaaaua cuaauaggca cacuccuaau guggcucgga     2040

cuaaacacca aaaacggauc aaucucccua augugucucg cccuaggcgg aguacucaua     2100

uuucuaucca cagcagucag cgcagacgua ggguguucag ucgacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5065
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 1 opt

<400>  5065
gggagaaagc uuaccaugcg aaucauaaac gcccggaaag aaaagaaacg ccgcggagcc       60

gacaccuccg ucggaauagu aggccugcug cucaccacgg ccauggcugc cgaagugacc      120

cgccgaggaa gugccuacua cauguaccuc gacagaaacg acgcaggcga agcaaucagc      180

uuccccacaa cguuaggcau gaacaaaugc uauauacaga uaauggaccu gggccauaug      240

ugugaugcaa ccaugucaua cgaaugcccc augcucgacg aaggagucga accagacgac      300

guagauugcu ggugcaacac aaccucgaca uggguagucu auggaacaug ccaccacaag      360

aaaggcgaag cccgccgcuc aagacgagcg guaacgcuac cuucgcauuc cacacgaaaa      420

cuacaaaccc gcucccaaac auggcuagaa agccgagaau acacaaaaca ccucaucagg      480

guagagaacu ggaucuuccg caacccaggc uucgcacucg ccgcagcagc caucgccugg      540

cuacuaggaa gcagcacauc gcaaaaaguu aucuaccucg uaaugauccu ccuuaucgcc      600

ccggccuaca gcauacgcug caucgggguc uccaacagag acuucgucga aggcaugagu      660

gggggcacgu ggguagacgu cguacuagaa cacggcggau gcgugaccgu cauggcacaa      720

gacaaaccca ccguggacau agagcuagua acaaccacug uaucgaacau ggccgagauc      780

agaagcuacu gcuacgaagc cuccauaucc gacauggcca gcgacagcag augcccgaca      840

caaggagaag ccuaccucga caaacaaagc gacacacaau augucugcaa acgcacccua      900

gucgacagag gcuggggcaa cgggugcggc cucuucggca aaggaagccu cgucacaugc      960

gcgaaauucg caugcuccaa aaagaugacc ggaaaaucga uacaacccga gaaccucgaa     1020

uaccgaauca ugcuaagugu ccacggcagc caacacucag gaaugaucgu caaugacacc     1080

ggacacgaga cagacgaaaa ccgagccaaa guagaaauaa ccccaaacuc accacgggca     1140

gaggcgacgc uaggcggauu cggcucccug ggccucgacu gcgaaccacg gaccggacuc     1200

gacuucagcg accuauacua ccucacgaug aacaacaaac acuggcuagu acacaaagaa     1260

ugguuccacg acaucccacu gccauggcac gcaggcgcag auacaggcac accccacugg     1320

aacaacaaag aggcccuagu agaguucaaa gacgcacacg ccaaaagaca aaccguagug     1380

guacucggca gccaagaagg cgcaguacac acagcacucg ccggcgcacu ggaagcagaa     1440

auggacggag ccaaaggccg gcuauccagc ggccaccuga aaugccgccu caaaauggac     1500

aagcucaggc ucaagggagu aagcuacagc cuaugcaccg cagcauuuac cuucacaaaa     1560

auccccgcgg aaacucugca cggcaccguc acagucgagg uucaauacgc aggcaccgac     1620

ggcccaugca agguccccgc acaaauggcu guggacaugc aaacacucac cccugucggc     1680

agacucauaa ccgccaaccc agucauaacc gagagcacag aaaauuccaa aaugaugcua     1740

gaacuagacc cucccuucgg ggacagcuac aucgucaucg gcguagggga gaaaaagauc     1800

acccaccacu ggcaccgguc aggcucaacc auaggcaaag ccuucgaagc aacaguaaga     1860

ggagccaaac gcauggccgu acugggagau acagccuggg auuucggauc ugucggaggc     1920

gcacuuaaca gccuuggcaa aggcauccac caaaucuucg gcgccgccuu caagucccuc     1980

uucggcggaa ugagcugguu cagccaaauc cuaaucggca cccuccuaau guggcucgga     2040

cucaacacca aaaacgguuc caucucauua augugccuag cccucggagg uguccucaua     2100

uuccuaucca ccgcagucuc cgccgacgua ggcuguagcg ucgacuucuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5066
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 1 opt

<400>  5066
gggagaaagc uuaccaugcg caucaucaac gcaagaaaag aaaagaaacg caggggagca       60

gacaccucgg ucggcauagu cggacuccua cucaccaccg ccauggcugc ugaaguuaca      120

cgcagaggau cggccuacua cauguaccug gaccgcaacg acgcagggga ggccauaucg      180

uuccccacca cacuuggaau gaacaaaugc uacauacaaa ucauggaccu cgggcacaug      240

ugcgacgcaa ccauguccua cgaaugcccc augcucgacg aaggaguaga accagacgau      300

gucgacugcu ggugcaacac cacgagcaca ugggucguuu acggcacaug ccaccacaag      360

aaaggugaag cccgccgcag ucgacgagcc gucacccuuc caagccacuc cacacgaaaa      420

cuccaaacca ggagccaaac cuggcucgaa agccgagaau acaccaaaca ccucauaaga      480

guagagaacu ggauauuccg caacccaggc uucgcccucg cagccgccgc caucgcgugg      540

cuccuaggca guagcaccag ccaaaaagug auauaccuag ucaugauacu acugaucgca      600

ccagcauacu caauccggug cauaggagua ucuaaccgag acuucguaga aggcauguca      660

ggagggacau ggguggacgu aguccucgaa cacggagggu gcguaacagu aauggcccaa      720

gacaaaccca ccgucgacau cgagcuaguc accacgaccg ucagcaacau ggcagaaguc      780

cgguccuacu gcuacgaagc aagcaucucg gacauggcca gugacucccg cugccccacg      840

cagggcgaag cauaccucga caaacaaucc gacacccaau acgucuguaa acgcacacuc      900

guagaccgcg gcuggggaaa cggcugcggg cuauucggaa agggcagcuu agucacaugc      960

gccaaguuug caugcucaaa gaaaaugacc ggcaaaagca uacaacccga aaaccucgaa     1020

uaccguauca ugcuguccgu acacggauca caacacagcg gaaugaucgu caacgacacc     1080

ggccacgaaa cagacgaaaa cagagcaaaa gucgaaauaa cucccaauag cccacgcgca     1140

gaggccacac ugggcgguuu cggcagccug ggccuagacu gcgaaccccg cacaggccuc     1200

gacuuuuccg accucuacua ccucaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccaug acaucccccu cccauggcac gccggcgccg acaccggaac cccacacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcccacg cgaaacgaca aaccguagua     1380

guacucggca gccaagaagg cgcaguucau acggcccucg cgggagcacu cgaagcugaa     1440

auggacggcg caaaaggacg acucucauca ggacaucuga aaugccgacu caaaauggac     1500

aaauuaagac ucaaaggcgu auccuacucg cucugcacgg cagcauucac guuuacaaaa     1560

auaccagcag aaacccugca cggcaccguu accguagaag uccaauacgc cgguaccgac     1620

ggcccgugca agguccuagc acaaauggca gucgacaugc aaacauugac acccgucggc     1680

cgacugauca cagcaaaccc cgugaucacc gaaucaaccg aaaacuccaa aaugaugcuc     1740

gaacuggacc caccauucgg cgauagcuau aucgucaucg gagucggcga aaagaaaauc     1800

acccaccacu ggcaccgauc cggcuccaca aucggaaaag cauucgaagc aacaguacgg     1860

ggcgcaaaac ggauggcagu ccucggcgac acagccuggg acuucggcag cgucggcggc     1920

gcccucaaca gccuaggcaa aggcauccac caaauauucg gugcagccuu caagagccuc     1980

uucggaggaa ugagcugguu cagccaaaua cucaucggca cacuccuaau guggcugggc     2040

cucaacacca aaaacggcag cauaagccuc augugucugg cccuaggagg aguacuaaua     2100

uuccuaucca cugccgucag cgcagacguu ggcugcagcg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5067
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 1 opt

<400>  5067
gggagaaagc uuaccaugcg caucaucaac gcacgaaagg agaaaaaacg ccgaggggcc       60

gauaccuccg uuggcaucgu gggacuccuc cuaacuacag cuauggccgc ggaagugacc      120

cgccgcggaa gcgcuuacua cauguaccua gaucgcaacg acgccggcga agccaucuca      180

uucccaacga cacugggcau gaacaaaugc uacauccaaa ucauggaccu aggccacaug      240

ugcgacgcca ccaugucuua cgaauguccc acacuugacg aaggcguaga gcccgacgac      300

guagacugcu ggugcaacac gacgucaacc uggguuguau acggcacaug ccaccacaaa      360

aagggagaag ccagacgcag cagaagagcc gucacacuac ccagccacuc gaccagaaaa      420

cuacagaccc gcagccaaac auggcuggaa ucccgcgaau acacaaaaca ccuaauacgc      480

guagaaaacu ggauauucag gaacccgggc uucgcccuag ccgcagcagc aaucgccugg      540

cuccuaggcu ccuccacauc ccaaaaaguc auauaucucg ucaugaucuu acucaucgcc      600

ccagccuacu cuauacgcug caucggcguc uccaaccgcg acuucgucga aggcaugucc      660

ggaggaacau ggguagacgu aguacuggag cacgggggau gcgucacagu aauggcccaa      720

gacaaaccca ccguagacau agaacuaguc accaccacag ucucaaacau ggccgaaguc      780

cgcucauacu guuacgaagc aagcauaucc gacauggccu ccgacucgcg gugcccaacc      840

caaggcgagg ccuaccuaga caaacaaagc gacacccaau acgucugcaa gcguacgcug      900

guggaccgug gauggggaaa cggaugcgga cuauucggca aaggcagccu cguuaccugc      960

gcaaaauucg ccugcuccaa aaaaaugaca ggcaaaucga uucaacccga gaaccucgaa     1020

uaccgaauaa ugcuaagcgu ccacggauca cagcacuccg gcaugaucgu caacgacacc     1080

ggacacgaga cagacgagaa ccgcgcaaaa guggaaauca ccccgaacuc cccccgugca     1140

gaggcaaccc uaggcggcuu cggcucacuc ggacucgacu gugaacccag aaccggccuc     1200

gacuucuccg acuuguacua ccucacaaug aacaacaaac acuggcuagu ccacaaagaa     1260

ugguuccacg acaucccccu ccccuggcac gcaggagcgg acaccggaac cccacacugg     1320

aacaacaaag aagcccucgu cgaguucaaa gaugcccacg caaaacgcca gacagucguc     1380

guccuaggaa gccaagaagg agcaguacac acagcacucg caggagcccu agaagccgaa     1440

auggacggug caaaaggcag acucagcagc ggacaucuca aaugcagacu uaaaauggac     1500

aaacuccggc ucaaaggcgu cagcuacagc cucugcacag cagccuucac auucacaaaa     1560

auccccgcgg aaacccucca cggcacugua acggucgaag uccaauacgc aggaacggau     1620

ggcccaugca aagucccagc ccaaauggca gucgacaugc aaacccucac ccccgucggc     1680

cgucucauaa ccgcaaaccc ggucaucaca gaaagcaccg aaaacagcaa aaugaugcug     1740

gaacuagacc cacccuucgg agauuccuac aucgucaucg gcguaggcga aaaaaaaaua     1800

acccaccacu ggcacagauc agguucgaca aucggaaaag cguucgaagc caccguaaga     1860

ggcgccaaac gcauggccgu ccucggagac accgcauggg acuucggauc uguaggugga     1920

gcccuuaacu cccuaggcaa agguauccac caaaucuuug gagcugcauu caaaucacua     1980

uucggcggaa ugucaugguu cagucaaaua cucaucggga cccuacucau guggcuaggc     2040

cuaaauacca aaaacggguc gauaucacua augugccucg cacucggcgg cguacucaua     2100

uuucucucaa ccgcgguaag cgcagacgug ggaugcucag ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5068
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 1 opt

<400>  5068
gggagaaagc uuaccaugcg aauaauaaau gcacgaaagg aaaagaaacg aagaggagca       60

gacacauccg uaggaauagu gggacuacua cuaaccacgg ccauggcagc agaaguaacc      120

aggaggggau cggccuacua cauguaccuc gacagaaacg acgcaggaga agcaaucucc      180

uuccccacaa cacucggaau gaacaaaugc uacauccaga uaauggaucu uggacacaug      240

ugcgacgcca caaugagcua cgagugcccc auguuagacg aaggcguuga accagacgac      300

gucgauugcu ggugcaacac aaccucaacu uggguagucu acgggacaug ccaucacaag      360

aaaggggagg cccgcagguc cagacgagcc guaacacucc ccagccauuc aacacgcaaa      420

cuccagacgc gcagccagac cuggcucgaa agccgagaau acacaaaaca ccuaauccgc      480

guagaaaacu ggauuuuuag aaaccccgga uucgcacucg cagcagcggc uauagccugg      540

cuccucggau ccagcaccag ccaaaaagua aucuaccucg uaaugauccu gcucaucgcc      600

ccagcauaca gcauccgaug caucggagug agcaacagag acuucguaga aggcauguca      660

ggcggcaccu ggguagaugu aguacucgaa cacggaggau gcguaacggu aauggcccaa      720

gacaaaccaa caguggacau cgaacucgug accaccaccg ucuccaacau ggcagaagua      780

cggucauacu gcuacgaagc cucaaucagc gacauggcaa gcgacuccag guguccgacc      840

caaggcgaag cauaccucga caaacaaagc gacacccaau acgucugcaa acggacccua      900

guagaccgag gcugggguaa cggcugcggc cucuuuggaa aaggaagccu cguaacaugc      960

gccaaauuug ccugcagcaa aaagaugaca gggaaaagca uacaaccaga aaaccuagaa     1020

uacagaauaa ugcucuccgu ccacggcagc caacacucag gcaugauagu aaacgacacc     1080

ggacacgaaa cggacgagaa cagagccaaa gucgaaauaa ccccaaacag cccacgcgcc     1140

gaagccacgc ucgggggcuu cggaucauug ggauuagacu gugaaccccg cacaggucuc     1200

gauuucagcg accucuauua ccucaccaug aacaacaagc acuggcuggu acacaaagaa     1260

ugguuccacg acauaccacu ccccuggcac gccggugcag acaccggcac accccacugg     1320

aauaauaaag aagcguuggu cgaguucaaa gaugcccacg cgaagagaca aaccguagua     1380

gugcucggca gccaagaagg agccguacac accgcccucg ccggcgcucu cgaagccgaa     1440

auggacggag ccaaaggucg ccugagcucc ggccaccuaa aaugccgacu aaaaauggac     1500

aaacuuagac uaaaaggagu aucauacuca cucugcacgg cagccuuuac cuucaccaaa     1560

aucccggcag aaaccuuaca cggaacagua acagucgaag ugcaauacgc aggcacagac     1620

ggccccugca aaguccccgc acagauggcc guagacaugc agacccucac cccggucgga     1680

cgccucauca cagcuaaccc ggugaucacc gaauccaccg aaaacagcaa aaugaugcuc     1740

gaacuugacc cacccuucgg cgacagcuac aucgucaucg gagugggaga aaagaaaauc     1800

acgcaucacu ggcaccguag cggaucaacg auaggaaagg ccuucgaggc cacgguaaga     1860

ggcgcgaaaa gaauggcagu ccuaggagac acagccuggg auuucggguc ggucgguggc     1920

gcacucaacu cacuaggcaa aggcauccau caaaucuucg gcgccgccuu caaaagccua     1980

uucggaggaa ugagcugguu uagccaaaua cuuauaggca cacuccuaau guggcucgga     2040

cucaauacaa agaacggaag caucucacua augugucuag cccuaggcgg gguccucaua     2100

uuccuaucca cagucgucag cgccgacguc ggcugcagcg uugacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5069
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 1 opt

<400>  5069
gggagaaagc uuaccaugag aaucaucaau gccagaaaag aaaagaaacg gcgcggagcc       60

gacacauccg ucgggauagu aggacuccug cucacaaccg caauggcagc ggaggucacu      120

cgacggggcu cagcguacua cauguaccuc gacagaaacg acgcaggaga agcaauauca      180

uuccccacaa cacuaggaau gaacaaaugc uauauccaga uaauggaccu aggccacaug      240

ugcgacgcga caaugagcua cgagugucca augcucgacg aaggaguaga acccgacgac      300

guagacugcu ggugcaacac caccuccgca uggguagugu acggaacaug ccaccacaag      360

aaaggcgaag ccagacgcuc gcgcagagcc gucacccuac ccagccacuc cacccgaaaa      420

cuucaaaccc gcccacaaac auggcuagaa ucacgggaau acaccaaaca ccuaauacgc      480

guugaaaauu ggauauucag aaaccccgga uucgcacuag cugccgcugc cauagccugg      540

cuacuaggga guucaacauc acaaaaaguc aucuaccuag ucaugaucuu acucaucgca      600

cccgccuaca gcauccgcug uauaggaguc agcaaucgag acuuuguaga gggaaugagc      660

gggggcaccu ggguagacgu cguccuagaa cacggcggau gcguaaccgu cauggcgcaa      720

gacaaaccaa ccgucgacau cgaacucguc acaacaaccg ucucaaacau ggccgaaguu      780

cgaucauacu gcuacgaagc gaguauauca gacauggcca gcgacucccg gugccccacc      840

caaggugaag cauaccugga caaacaaagc gacacccagu acgucugcaa aaggacccug      900

guggaccgcg gauggggcaa cggcugcgga cucuucggca aaggcagccu cguuacgugc      960

gcuaaauucg ccugcagcaa gaaaaugaca ggcaaaucca uccaacccga aaaccuagaa     1020

uaccgaauca ugcuaagcgu acacggcucg caacacucag gcaugauagu caacgacacc     1080

gggcacgaaa ccgacgaaaa ccgagccaaa guagaaauca cgccaaacuc accccgagcc     1140

gaggccacac ucggcggcuu cggaucccuc ggccuagacu gcgaacccag aacaggucuc     1200

gacuucuccg accuauacua ccugacaaug aauaauaaac acuggcucgu ccacaaagag     1260

ugguuccacg auaucccacu gcccuggcau gccggcgcgg acaccggcac accccacugg     1320

aacaacaaag aagcccuagu agaguucaaa gacgcacacg ccaaaaggca aaccgucguc     1380

guccuaggau cccaagaagg cgccguccac accgcccucg ccggcgcccu agaagcagaa     1440

auggacggag ccaaaggccg ccucucauca ggccaccuca agugccgccu aaaaauggac     1500

aagcuacgac uaaaaggcgu gagcuacucc cucugcacag cggccuucac cuucacgaag     1560

auaccagccg aaacacucca cgggaccguc accguagagg uacaauacgc aggaacagac     1620

gggcccugca aaguacccgc ccaaauggcc guagacaugc aaacccucac cccaguagga     1680

cgacucauaa ccgccaaccc agucauaacc gagagcaccg aaaacagcaa aaugaugcua     1740

gaacuagacc cacccuucgg cgauaguuac auuguaauag gcguaggcga aaagaaaauc     1800

acacaccauu ggcacagauc cggaucaacc auuggcaaag cauucgaagc aaccguacgc     1860

ggcgcuaaac gcauggccgu acucggcgau accgcauggg acuuuggauc cgucggggga     1920

gcauuaaacu cacuaggaaa aggcauccac cagauauucg gcgccgcauu caaaucacuc     1980

uucggaggaa ugucaugguu uagccaaaua cucauaggca cacuccucau guggcuaggc     2040

cucaacacca aaaacggaag cauaucccuu augugccucg cccucggcgg uguucucauc     2100

uuucuaucca cagccgucag cgccgacguc ggcugcucag ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5070
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 1 opt

<400>  5070
gggagaaagc uuaccaugcg caucaucaac gcccgcaaag aaaagaaacg acgcggcgcu       60

gacaccuccg uaggcauagu aggacuccua cucaccaccg caauggcagc agaagucacc      120

cgccgaggca gcgcauacua cauguaccuc gacagaaacg acgccgguga agccauauca      180

uuccccacca cccucggaau gaacaagugc uacauccaaa uaauggaccu uggccacaug      240

ugcgacgcca ccaugagcua cgaaugccca augcucgacg aaggaguaga gccagacgac      300

gucgacugcu ggugcaacac gaccuccacu ugggucgucu auggcacgug ccaccacaag      360

aaaggagaag ccagaagauc acgccgugca guaacgcuac cgagccacuc aacccgaaaa      420

cuucaaaccc guagccagac cuggcuagag ucacgcgaau acacuaaaca ccugauccgc      480

guagaaaacu ggauauuucg caaccccggc uucgcgcucg cagccgcggc aauagccugg      540

cugcuaggau cguccaccuc caaaaagguu auauaccucg uaaugauccu ccucauagcu      600

cccgcauacu caauaaggug cauaggcguc agcaaccgcg acuucgucga aggcaugucc      660

ggaggcaccu ggguagacgu gguccucgaa cacggaggau gcgucacagu gauggcacaa      720

gacaaaccca ccgucgacau agaacucguc accacuacag ucagcaacau ggccgaaguc      780

agaucauacu gcuacgaagc aagcaucagc gacauggcau ccgacucgcg cugccccacc      840

caaggcgaag cauaccuaga caaacaaagc gacacccagu acguaugcaa acgcacacuc      900

gucgaccggg gcugggggaa cggcugcggu cucuucggaa aaggcucccu cguaaccugc      960

gccaaauucg caugcuccaa gaaaaugacc ggcaaaagca uccaacccga aaaccucgaa     1020

uaccgaauca ugcuaucggu ccacgguucc caacacagcg gaaugauagu caacgacacc     1080

ggacacgaaa ccgacgaaaa ccgagccaaa gucgaaauaa cacccaacag cccgcgagca     1140

gaagccacac uaggcggauu cggaagccuc ggccuagacu gcgagccccg cacaggauua     1200

gacuuuuccg acuuauacua ccuaacgaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccacu accauggcac gcaggggccg acaccggaac accacacugg     1320

aacaauaaag aggcacucgu agaauuuaaa gacgcacacg caaaacgaca gaccguuguc     1380

guacucggau cccaggaggg agccguccac accgcacuag ccggugcccu agaagcagaa     1440

auggacggag caaaaggaag acuaaguagu gggcaccuca aaugccgucu aaaaauggac     1500

aagcucaggc uaaaaggcgu gagcuacucc cucuguacag ccgccuucac cuucaccaaa     1560

auccccgcug aaacacuaca cggaaccguc accgucgaag uccaauacgc cggcaccgac     1620

ggccccugca aaguucccgc ccagauggcc gucgacaugc aaacccucac acccgucggu     1680

cgacucauca cagcgaaccc cgugauaacc gaaagcacug aaaauagcaa aaugaugcuc     1740

gagcuggacc cacccuuugg ggacagcuac auaguaaucg gcgucggcga aaagaaaauc     1800

acccaccacu ggcacagaag cgggagcacc auaggaaaag cauucgaagc aacaguccgc     1860

ggugcaaaac guauggccgu acucggagac acagccuggg acuucggcag cguaggcggu     1920

gcacuaaaca gccuaggaaa aggaauacac caaauauucg gagccgccuu caaaagccuc     1980

uucggaggca ugucgugguu cucgcagauu cucauaggca cacuucuaau guggcuaggc     2040

cuaaacacca agaacggcuc aaucagccua augugccugg cacucggagg cguccuaauc     2100

uuccuaucaa cagcagucag cgcagacguc ggaugcagcg uagacuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5071
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 1 opt

<400>  5071
gggagaaagc uuaccaugag aaucaucaac gcacgaaaag aaaagaaacg acgcggagcc       60

gacacauccg uaggcaucgu uggacuacua uuaaccacag caauggccgc cgaaguaaca      120

agaagaggua gcgcauacua cauguaucuu gaccggaacg acgccggcga agccaucagc      180

uucccgacca cacuagggau gaacaaaugc uacauucaaa uaauggaccu gggccauaug      240

ugcgacgcaa caaugucaua cgaaugcccg augcucgacg agggcguaga accggacgac      300

gucgauugcu ggugcaacac aacuucaacu ugggucgucu acgguaccug ccaccacaag      360

aaaggcgaag cccgccgcuc gcggcgcgca guaacgcuac ccagccacuc cacgcgcaaa      420

cuacaaaccc gcucccagac cuggcuagaa ucgcgagagu acacaaaaca ccuaauccgc      480

guggaaaacu ggauuuuccg aaacccaggc uucgcccucg cagccgcagc gaucgcaugg      540

cuccuaggca guagcacacg acaaaaagua auauaucuug uaaugauacu ucucaucgcc      600

cccgccuaca gcauccgaug cauuggcguc agcaacaggg acuucguaga aggcaugucc      660

ggaggaaccu ggguagacgu cguucucgaa cacggcggcu gcgucaccgu aauggcacaa      720

gauaaaccga ccguagacau cgaacuugug accaccacgg uguccaacau ggccgaagua      780

agaagcuacu gcuaugaagc aagcaucagc gacauggcca gcgacagcag augccccaca      840

caaggcgagg cuuaccucga uaaacaauca gacacccagu acguaugcaa gcgaacccua      900

gucgacagag gcuggggaaa cggcugcggc cucuucggua aagggucacu cguuaccugc      960

gccaaauucg ccugcagcaa gaaaaugacc ggcaagagca uccaacccga aaaccucgaa     1020

uaccgaauca ugcugucagu ccacgguagc caacacucag guaugauagu caacgacacc     1080

ggacacgaaa cagacgaaaa cagagcaaaa gucgaaauca cacccaacag cccuagagcg     1140

gaagccaccc ucggaggcuu cggcagccua ggccuagacu gugaaccacg cacaggccuc     1200

gacuucagcg acuuauauua ucucacaaug aacaacaagc acuggcuagu ccacaaagaa     1260

ugguuccacg acauaccacu acccuggcac gcaggagcag acaccggcac accccacugg     1320

aacaacaaag aggcacucgu ugaauuuaaa gacgcacacg ccaaacgaca aaccgucgua     1380

guacuaggaa gccaagaagg cgcaguacac acagcccuag ccggggcgcu agaagccgaa     1440

auggacggcg ccaagggacg ccucuccucc ggacaccuca aaugucggcu aaaaauggac     1500

aaacuaagac uaaaaggggu auccuacagc cucugcacgg cagccuucac cuuuaccaaa     1560

aucccagccg aaacccuaca cgggacaguc acaguagaag uucaauacgc cgggaccgac     1620

ggaccaugca agguccccgc gcaaauggcc guggacaugc aaacccuaac accuguaggc     1680

cgacucauaa ccgccaaccc cguaauaacc gaaagcaccg aaaacuccaa aaugaugcuc     1740

gaauuagauc ccccauucgg cgacagcuac auaguaauag gcguaggcga gaaaaagauc     1800

acgcaccacu ggcacagauc cggcagcacc aucggaaaag ccuucgaggc aaccgugcga     1860

ggcgccaaac gcauggccgu ccucggagac acggcauggg acuucggauc aguaggcgga     1920

gcccuaaaca gccuaggaaa aggaauacac caaauauucg gcgcagccuu caaauccuua     1980

uucggcggca ugagcugguu cucacaaaua cuaaucggga cccugcucau guggcucgga     2040

cucaacacca aaaacggaag cauaucccuc augugccucg cacucggggg cguacuaauc     2100

uuccugagca cagcaguauc cgccgacgua gguugcucgg ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5072
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 1 opt

<400>  5072
gggagaaagc uuaccaugcg cauaaucaac gcgcgaaaag aaaagaagcg aagaucggca       60

gacaccagcg ucggcauagu cggccuacuc cucaccaccg cgauggccgc agaagucaca      120

cgcaggggcu ccgcauacua cauguaccuc gaccgaaacg acgccggcga agccaucagu      180

uuccccacaa cacuaggaau gaacaaaugc uacauacaaa ucauggaccu ggggcacaug      240

ugcgacgcca caauguccua cgaaugcccc augcuggacg aaggcgugga accggacgac      300

guagacuguu ggugcaauac caccagcacc uggguagugu auggcacaug ccaccacaag      360

aaaggagaag ccagacgcuc acggcgcgca gugacacucc caagccacag cacucguaaa      420

cuacaaaccc gaagccaaac cuggcuagaa ucccgagaau auacaaaaca ccucauaaga      480

guagaaaacu ggauauuccg aaaccccgga uucgcgcucg cagccgcagc aauagcaugg      540

cuacuuggcu caagcaccag ccaaaaaguc auauaccuag ucaugauacu gcucaucgcc      600

ccagcauacu caaucagaug cauaggcgua agcaaccgag acuucguuga agggaugucu      660

ggagggacau gggucgacgu cguacuagaa cacggaggcu gcgucacggu cauggcccag      720

gacaaaccaa cgguagauau agagcucgua acgacaaccg uaagcaacau ggcagaagua      780

cgcaguuacu gcuacgaagc cagcauauca gauauggccu ccgacucccg cugccccaca      840

cagggagagg ccuaccucga caaacaauca gacacacaau acgucugcaa acggacgcua      900

guagaccgcg gcuggggaaa uggaugcggu cucuucggca aaggaucgcu agugacaugu      960

gccaaauucg caugcuccaa gaaaaugaca ggcaaaucca uccaaccaga aaaccuagag     1020

uacagaauaa ugcucagcgu acacggcagc caacacagcg gcaugauagu caaugacaca     1080

ggacacgaaa ccgacgaaaa ccgggccaag guagagauaa cccccaacag cccaagagca     1140

gaggcgaccc ugggcgguuu cggcagccuc ggccuagacu gcgagccaag aacaggacuc     1200

gacuucagcg accucuacua ccuaacaaug aacaacaaac acuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu accauggcac gccggcgcag acaccgggac accacacugg     1320

aacaacaagg aagcccuagu cgaguucaag gacgcacacg caaaacgaca gaccgucgua     1380

guacuaggau cacaagaagg ggccguacac acagcccuag ccggugcacu agaagcagaa     1440

auggacggag caaaaggacg ccuaucaagc ggccaccuaa aaugccgccu caaaauggau     1500

aaacuucguc uaaaaggagu cagcuacagc uuaugcacug cagcauucac auucacaaaa     1560

aucccagcag aaacacuaca cggaaccguc acaguggaag uccaauacgc cggaacagac     1620

ggcccaugca aaguaccagc ccaaauggcg gucgauaccc agacacucac cccagucggg     1680

cgacucauaa cagcaaaccc agucaucacc gaaucgacag aaaacucaaa aaugaugcua     1740

gaacucgacc cacccuucgg cgacagcuau aucguaauag ggguaggaga gaaaaagaua     1800

acacaccacu ggcaccggag cggcagcacc auaggcaaag cguucgaagc cacaguccgu     1860

ggagcaaaaa gaauggcagu auugggggau acggccuggg acuucggcag cguaggagga     1920

gcccucaaca gccuaggaaa agggauccac caaauauucg gcgccgcguu caaaagccug     1980

uucggaggaa ugagcugguu cucccaaaua cuaaucggga cccuccucau guggcucgga     2040

cuuaacacca aaaacggaag caucucacua augugccuag cacuaggagg agugcuaauu     2100

uuccugagua cagccguauc cgcggacguc ggcugcuccg uugacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5073
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 1 opt

<400>  5073
gggagaaagc uuaccaugag aaucaucaac gcaagaaaag aaaagaaaag acgaggagcc       60

gacacaaacg uaggaaucgu cggacuacuc cucacaacug caauggcagc agaagucacg      120

cgacgaggau ccgccuacua cauguaccua gaccgcaacg acgcgggcga agcaauauca      180

uucccaacca cccuggggau gaacaaaugc uacauacaga uaauggaccu cggacacaug      240

ugcgacgcga ccaugagcua cgagugccca augcucgacg aaggcgugga accagacgau      300

gucgacugcu ggugcaacac cacgagcaca ugggucguau acgggacaug ccaccacaag      360

aaaggagaag cgaggcgauc ccggagagcc gucacccucc cgagccacuc aacacgaaaa      420

cuacaaacaa gaucgcaaac cuggcuagaa ucgagagaau auaccaaaca ccuaauaagg      480

guagaaaacu ggaucuuccg caacccgggc uucgcgcuag ccgcagccgc aauagccugg      540

cugcuaggga gcucaacaag ccagaaagua aucuaucuag uaaugauccu ccuaauagcc      600

ccagcauaca gcauccgcug cauaggcgua agcaacaggg auuucguaga aggaaugagc      660

ggaggcacau ggguagacgu agugcucgag cauggaggau gcgucacagu cauggcacaa      720

gacaaaccaa cagucgacau agaacuggua acaaccacag uguccaacau ggcagaaguc      780

cgcagcuacu gcuacgaagc cagcauaagc gacauggcaa gcgacagccg gugcccaaca      840

caaggagaag ccuaccuaga caaacaaucc gacacacaau acguuugcaa acgcacguua      900

guagaccgcg gauggggaaa cgguugcgga cucuucggca aagguagcuu ggugacaugc      960

gccaaauucg ccugcaguaa gaaaaugaca ggcaaaagca uccaaccaga gaaccuagag     1020

uacagaauca ugcucagcgu ccacggcucc caacacagug gaaugaucgu aaacgacaca     1080

ggacacgaga cggacgaaaa cagagcaaaa guagaaauca cacccaacag cccacgggca     1140

gaagccaccu ugggaggauu cggaucccua ggucucgacu gcgaacccag aaccggauua     1200

gauuucuccg accucuacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acaucccauu accauggcac gccggugccg acacaggaac cccgcacugg     1320

aacaacaaag aagcccuagu ggaguucaaa gacgcccacg caaaaagaca aacaguagug     1380

guucuaggau cccaagaagg ugcaguccac acugcacugg cgggugcacu cgaagccgag     1440

auggacggcg caaaaggccg gcuaucgagc ggccaccuaa aaugcaggcu caagauggac     1500

aagcuaagac ucaaaggcgu gagcuacuca cucugcaccg cagcguucac auucaccaaa     1560

aucccagccg aaacccuaca cgggacaguc acaguggagg uccaguaugc aggcaccgau     1620

ggcccaugca aaguaccggc ccaaauggca guagacaugc agacacuaac accggucggc     1680

cgacuaauaa cagcaaaccc aguaaucaca gaaagcaccg agaacucgaa aaugaugcua     1740

gaacucgauc ccccauucgg cgacagcuac aucguaaucg ggguagggga gaagaaaauc     1800

acccaccauu ggcaccggag cggcagcacg aucgggaaag cauucgaagc cacgguacga     1860

ggagcgagac gaauggcagu ccucggggac accgcauggg acuucggcag uguuggcgga     1920

gcauugaacu cgcucgggaa agggauacac cagauuuucg gggcagcauu caaaucacua     1980

uucggaggga ugagcugguu cagccagauc cucaucggaa cccuacugau guggcuugga     2040

cuaaacacca aaaacggauc cauaagccua augugccuag cccugggagg aguccuaaua     2100

uuccuaagua cagccguaag ugccgacgua ggaugcagcg ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5074
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 1 opt

<400>  5074
gggagaaagc uuaccaugcg aaucauaaac gcccgaaaag aaaagaaacg acgcggagca       60

gaaaccucgg uaggcauagu aggacugcua cuaaccacag cgauggcagc cgaaguaaca      120

agaagagguu cagccuacua cauguaccuc gaccgcaacg acgcaggcga agcgaucagc      180

uucccaacga cgcuaggaau gaacaaaugc uacauacaaa uaauggaccu gggccauaug      240

ugcgacgcca cgaugaguua cgaaugcccc auguuggacg agggagucga gccagacgac      300

gucgacugcu gguguaacac aacgagcacg ugggucgucu acggaaccug ccaccacaag      360

aaaggcgaag cccgacgauc aagacgcgca guaacacuac cgagccacuc cacacgcaaa      420

cuccaaacaa gaagccaaac cuggcucgaa agccgagaau acacaaaaca ccuaauccgc      480

guagaaaacu ggaucuuccg caacccaggc uucgcacuag cagccgccgc aauagcaugg      540

cuccucggua gcagcaccag ccaaaaaguc aucuaucuag uaaugauacu ccuuauagcg      600

ccagcauacu ccauucgaug caucggcguu aguaaccgcg acuucguaga aggaaugucg      660

gguggaaccu ggguagacgu gguacucgag cacgguggcu gcguaacggu gauggcgcaa      720

gacaaaccca cagucgacau cgaacucgug acaacaacag uaagcaacau ggccgaaguc      780

cggucauacu gcuacgaggc aucaaucagc gauauggccu cagacagccg cugccccacg      840

cagggcgaag ccuaccucga caaacagucc gacacacaau acgucugcaa acgaacgcua      900

gucgacaggg gaugggggaa cggcugcgga cucuucggca aaggaucccu aguaaccugc      960

gcaaaauucg ccugcucaaa gaaaaugaca ggaaaaagca uucaaccaga aaaucuagaa     1020

uacagaauca ugcugucagu ucacggcuca caacacagcg gcaugauagu aaacgacaca     1080

ggccacgaaa cagacgagaa ccgagccaaa guagaaauca cucccaauag cccaagagca     1140

gaagcaaccc uuggaggguu cgggucacuu ggacuagacu gcgaaccacg aaccggccua     1200

gacuucagcg accuuuacua ccucaccaug aacaauaaac acuggcucgu acauaaagaa     1260

ugguuccacg acaucccacu gccguggcac gcaggcgccg acacagguac accacacugg     1320

aacaacaaag aagcacuggu agaguucaaa gacgcccaug cgaaacgcca gaccgucguu     1380

gugcugggcu cacaagaagg agccguacac acagcacugg ccggagccuu agaagccgaa     1440

auggacggcg cgaaaggacg cuuguccucc ggccaccuca aaugccggcu aaaaauggac     1500

aaacuacgcc ugaaaggagu uucguauuca cugugcacag cagcguucac cuucaccaaa     1560

auaccagccg aaacccuaca cggcacagua accgucgaag uacaauacgc gggaaccgac     1620

gggcccugca aaguuccagc acaaauggcg guagacaugc aaaccuuaac accgguaggc     1680

agacuaauca cagccaaccc agucaucacc gaaaguacgg aaaauucgaa aaugaugcua     1740

gaacuagacc cgccauucgg agacagcuac auaguaaucg gggucggcga aaagaaaaua     1800

acacaccacu ggcacagaag cggcagcacg auaggcaaag ccuucgaagc caccgucaga     1860

ggcgcgaaac ggauggcagu acuaggcgac accgccuggg acuucggcag cgucggcgga     1920

gcccucaaca gcuuaggcaa gggaauccac caaaucuucg gcgccgcauu caagagccuc     1980

uucggcggca ugucgugguu cucccaaauc cucauaggca cucuacuaau guggcuagga     2040

cucaacacga aaaacggcag cauaagccug augugccucg cguuaggcgg aguccucaua     2100

uuccugagca cagccgucuc agcggacguc gggugcagcg uggacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5075
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 1 opt

<400>  5075
gggagaaagc uuaccaugag aauaaucaac gcccgcaaag agaaaaagcg cagaggcgca       60

gacacauccg uaggcauagu aggccuacuc cugacaacag caauggccgc cgaaguaacc      120

cgucgaggau cagccuacua cauguaucua gaccgaaacg acgccggaga agcuaucagc      180

uuccccacca cacucggcau gaauaagugu uacauccaaa ucauggaccu cggccacaug      240

ugcgacgcaa ccaugagcua cgaaugccca augcuggacg aaggcguaga acccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggaaccug ccaccauaag      360

aaaggggaag ccagacggag ccgacgugcg guaacgcucc ccagccacuc aacucgcaaa      420

cuccaaaccc gcagccaaac guggcucgaa agccgggaau acaccaaaca ccuaaucaga      480

guggaaaacu ggauauuccg aaaccccgga uucgcgcucg cagcagccgc gauagcuugg      540

cugcucggaa gcuccaccuc ccagaaagua auauaucuag ucaugauccu ccucauagca      600

cccgccuaca gccuccgcug cauaggcgua uccaaccgag acuucgugga aggcaugagc      660

ggcggcaccu ggguagaugu cguacucgaa cacggaggcu gcgucaccgu aauggcccaa      720

gacaaaccca caguagacau agaacuuguc acaaccaccg uauccaacau ggccgaagug      780

cgguccuacu gcuacgaagc cagcauauca gacauggccu cggacucacg augcccaacc      840

caaggcgaag ccuaccucga caaacaaagc gacacccaau acguaugcaa acgcacacuc      900

guagacagag gauggggcaa cgggugcggu cuuuucggaa aaggaucacu cgucacaugc      960

gcaaaauucg ccugcuccaa aaagaugacc ggaaaaucaa uccaaccaga aaaccuagaa     1020

uaccgcauca ugcucagcgu acauggcucc caacacuccg gcaugauagu caacgacacc     1080

ggacacgaaa ccgacgaaaa ccgcgcaaaa guagaaauca cacccaacag cccacgagcc     1140

gaagccaccc ugggaggguu cgggagccua ggacucgacu gcgaaccgcg aacgggacua     1200

gacuucuccg accucuacua ccuuaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acaucccauu accauggcac gccggcgcug auaccggcac cccacacugg     1320

aacaacaaag aagcccucgu cgaguucaaa gacgcucacg cgaaacgcca aaccguaguc     1380

guacucggcu cacaagaagg cgccgugcau accgcacuag caggggcacu agaagcagaa     1440

auggacggcg caaaaggccg ccucagcucu ggccaccuua aaugcagacu aaaaauggac     1500

aagcuacgcc uaaagggcgu gagcuacuca cugugcaccg cagccuuuac cuucaccaaa     1560

auccccgccg aaacucucca uggaaccgua acagucgaag uccaguacgc cggcacggac     1620

ggaccaugca aaguccccgc acaaauggcc guugauaugc aaacacugac acccguggga     1680

agacucauca ccgccaaccc agucaucacc gaaagcacag aaaacucaaa aaugaugcug     1740

gaauuagacc caccauucgg cgacucauac aucgugaucg gggucggaga aaagaaaaua     1800

acacaccacu ggcaccgcag cggaucaaca auaggcaaag cauucgaagc cacagucaga     1860

ggggcaaaaa gaauggcggu acuaggagac acagcauggg acuucggcuc agugggcgga     1920

gcccucaacu cccuuggaaa aggcauucac caaaucuucg gggcagcauu caaaucccua     1980

uucggaggaa uguccugguu cucgcaaaua cucaucggca cgcuccuaau guggcuagga     2040

cuaaacacca aaaacggaag uauaucccua augugccucg cgcucggagg cguacuaauu     2100

uuccugagca ccgcagucag cgcagaugua ggcugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5076
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 1 opt

<400>  5076
gggagaaagc uuaccaugcg cauaaucaau gcacgaaagg agaaaaagcg caggggagcc       60

gacaccaacg uaggcauagu cggccuacuu cuaacaacag caauggcggc cgaaguaaca      120

agacgcggau cagccuacua cauguaccuc gaccgaaacg acgccggcga agccaucagc      180

uuccccacca cccucggcau gaacaaaugc uacauacaga uaauggaccu cggucacaug      240

ugcgacgcca caaugucaua cgaaugcccc augcuagacg agggagugga accggacgac      300

gucgacugcu ggugcaacac caccagcacc uggguagucu acggaaccug ccaucacaag      360

aaaggcgaag ccagaagauc ccgacgcgcu guaacccucc ccagccacag cacucgcaaa      420

cuccaaacca ggucacaaac cuggcuagaa ucucgagagu acacaaaaca cuuaauacga      480

guagaaaauu ggauguuccg aaaccccgga uucgcccucg cggcagcagc aauagccugg      540

cuccucggau ccuccacauc gcaaaaaguc auauaccuug uuaugauccu acuaaucgca      600

ccggcauaca gcauccgcug caucggaguc ucaaacagag acuucgucga aggaaugucg      660

ggcggcacau gggucgacgu cguucuugag cacggaggau gcgucacggu cauggcucaa      720

gacaaaccua caguagacau agagcugguu acaacaaccg uguccaacau ggccgagguc      780

cgguccuacu gcuacgaggc auccaucagc gacauggccu ccgacucacg augcccaacc      840

caaggggagg cuuaccucga caaacaaagc gacacacaau acgucugcaa acgaacacug      900

guugauaggg gauggggcaa cggcugcggc cuuuuuggca aaggaagccu ggucacaugc      960

gccaaguucg ccugcagcaa gaaaaugacg ggcaaaagua uacaaccaga aaaccuggaa     1020

uaccgaauca ugcucagcgu ccacgguucc caacacuccg gcaugauagu aaacgacaca     1080

ggccacgaaa cagacgaaaa cagagcaaaa gucgaaauca cccccaacag cccacgggcc     1140

gaagccacac ugggcgguuu cggcagccuc ggacucgacu gcgaaccgcg cacgggacuc     1200

gacuucucag accucuacua cuuaacaaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauuccccu accauggcau gccggagcgg acaccggcac accccacugg     1320

aacaacaaag aagcacuggu cgaguucaaa gacgcacacg ccaaacggca aacagucguc     1380

guccuaggau cacaagaagg agcaguccac acggcacuag cgggcgcccu agaagccgaa     1440

auggauggcg caaaaggccg gcuaucaagc ggacaccuaa aaugcagacu aaaaauggac     1500

aaacuuagac ucaaaggcgu aagcuacagc uuaugcacag ccgccuucac cuucacgaaa     1560

auccccgcag aaacacucca ugguacaguc acgguagaag ugcaauacgc cggcacagac     1620

gggccaugca aagucccugc acaaauggca gucgacaugc agacccuaac cccagucggc     1680

cgacucauaa cggccaaucc agucaucaca gagucaaccg agaacagcaa aaugaugcuc     1740

gagcucgacc cgccauucgg cgacagcuau aucgucaucg gugucggcga aaagaaaauc     1800

acacaccauu ggcacaggag cgguagcaca auaggcaagg cauucgaagc aaccguccgc     1860

ggcgccaaac gcauggccgu ccuaggagac accgccuggg acuucggauc aguagggggc     1920

gcccucaaua gccugggcaa aggcauccau caaaucuucg gggcagccuu caaaagccuu     1980

uucgguggaa uguccugguu cagccaaauc cucauaggca cccuccuaau guggcucggc     2040

cucaacacga aaaacggguc cauaucccuc augugucuag cacuaggcgg aguccucauc     2100

uuccucucca ccgcggucuc ggccgacguc ggaugcucgg uagauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5077
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 1 opt

<400>  5077
gggagaaagc uuaccaugag aauaaucaac gcccgaaaag agaaaaagcg ccgaggcgcc       60

gacacaagcg ucggcaucgu cggacuucuc cuaacaacag ccauggccgc ggaaguaaca      120

agaaggggca gugcauacua cauguaccuc gaccgcaacg acgcaggaga agccaucagc      180

uuccccacaa cauuaggcau gaauaaaugc uacauacaga ucauggacuu gggccacaug      240

ugcgacgcca caaugagcua cgaaugcccc augcucgacg aaggcguaga accagacgac      300

gucgacugcu ggugcaacac aacaagcacg ugggugguau acggaaccug ccaccacaag      360

aaaggagaag caagacgaag ccguagagca gugacacucc ccagccacuc gaccagaaaa      420

cugcaaacac gcagccaaac cuggcucgaa ucacgagaau acaccaaaca ccucauccga      480

gucgaaaacu ggauacuacg gaaccccgga uuugcacucg cagcagccgc caucgcaugg      540

cuacucggcu caucgaccuc ccagaaaguc auauaccuug uaaugauacu ccuaaucgcg      600

cccgccuaca gcauccgaug caucggcgug ucuaaucgag acuucguaga aggcaugagc      660

ggaggcacau gggucgacgu cguacuggag cacggaggcu gcgugacggu aauggcacag      720

gacaaaccca cagucgacau agagcuaguc acaacgaccg ucuccaacau ggcagaagua      780

cgcagcuacu guuacgaagc cagcauaagc gacauggcca gugacagccg gugcccaacc      840

caaggggaag ccuaccuaga caaacaaagc gacacacaau acguaugcaa aagaacccuc      900

gucgaucgag gcuggggaaa cggaugcggc cucuucggga aaggaucccu cgugaccugc      960

gccaaauucg caugcagcaa gaaaaugacc ggaaaaagua uacaaccaga aaaccuagag     1020

uaucgcauaa ugcuaucagu ccacggcucc caacacuccg ggaugauagu caacgacacc     1080

ggccacgaaa cagacgaaaa ucgcgcaaaa gucgaaauca ccccaaacag cccaagagcu     1140

gaagccaccc uaggaggauu cggaucccuc ggccucgacu gcgaaccacg aacaggccuc     1200

gacuuuuccg acuuauauua ccucacaaug aacaacaaac acuggcuagu ccacaaggaa     1260

ugguuccacg acaucccacu acccuggcac gcaggcgcag acacaggcac cccacauugg     1320

aacaacaaag aagcacucgu ggaguucaaa gacgcucacg caaaacgcca aacaguagua     1380

guccuaggcu cacaagaagg ugccguccac acagcucucg ccggagcccu agaagcagaa     1440

auggauggag caaaaggccg ccugucgagc ggccaccuca aaugucgacu aaaaauggac     1500

aaacuacgcc uaaaaggcgu cagcuacagc uuaugcacag cagccuucac cuucacaaaa     1560

auaccggcgg aaacacucca cggcacgguc acgguugaag uccaauacgc cggcacagac     1620

ggucccugca aagucccagc acaaauggca gucgacaugc aaacccuaac cccaguaggg     1680

cgucuuauca cagccaaccc agucauuacc gagucaacag aaaacaguaa aaugaugcua     1740

gaacucgacc cacccuuugg ggacucguac aucgucaucg gaauaggaga aaagaaaaua     1800

acccaccacu ggcaccgaag cggcuccacc auaggcaaag ccuucgaagc aacaguaaga     1860

ggagcaaaac gcauggcagu acuuggagac accgcguggg acuucggaag cgucggaggc     1920

gcgcucaaca gccucggcaa aggcauccac cagaucuucg gcgccgcguu caaaagccug     1980

uucggaggaa ugucaugguu cucccagaua cuaaucggaa cccuccuaau guggcuaggg     2040

cugaacacga aaaacggauc gauaagccua augugucucg cccuaggagg cguacuaaua     2100

uuccucucca cagcaguaag cgcagacguc ggcugcuccg uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5078
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 1 opt

<400>  5078
gggagaaagc uuaccaugag gauaauaaac gcacgaaaag agaaaaagcg aagaggagcc       60

gacacauccg ucggaaucgu cggacugcua uuaacaacag ccauggccgc agaagucacc      120

cgcagaggcu ccgccuacua cauguaccua gaccgaaacg acgcggguga agcaaucucc      180

uuccccacca cacucgguau gaacaaaugc uacauacaga ucauggaccu cggccacaug      240

ugcgacgcaa cgauguccua cgaaugccca augcuagacg aaggcguaga acccgacgac      300

gucgacugcu ggugcaacac aaccaguaca ugggucguau acgggaccug ccaccacaag      360

aaaggagaag cccgaagauc ccggcgggca guaacccuac cgucacacuc cacacgcaaa      420

cuucaaacgc gaagccaaac cuggcuagag aguagggaau auacaaaaca ccuaauaaga      480

guagagaacu ggauauuccg caaccccgga uucgcgcucg cggccgccgc aauagcaugg      540

cuacucggca gcagcaccag ccagaaagua auauaccuag ucaugauccu acucauagcc      600

ccggcuuacu caauccgcug cauagggguc agcaaccgcg acuucguaga aggcaugagc      660

ggaggaacau ggguggaugu aguacuagag cacggcgggu gcguaaccgu aauggcacaa      720

gacaaaccua ccgucgacau cgaacucgua agcacaacug uauccaacau ggccgaagua      780

aggucuuacu gcuacgaagc caccauaucc gacaucgccu ccgacuccag augcccaacg      840

caaggggaag ccuaccuaga caaacaaucc gacacacaau auguaugcaa acgaacccua      900

gucgacagag gauggggaaa cggcugcgga cuauucggca aaggaagucu gguaaccugc      960

gcaaaauucg caugcuccaa gaaaaugaca ggaaaaucca uacaacccga aaaccucgaa     1020

uaccgcauca ugcuaagcgu ccacggaagc caacacagcg gaaugaucgu caacgacacc     1080

ggacacgaaa ccgacgaaaa ccgagccaaa guagaaauca caccaaacag cccccgcgcc     1140

gaagccaccc ucggcggcuu cggcucccua ggacuggacu gcgaaccacg aaccggccua     1200

gacuucagcg accuauacua ccuaaccaug aacaacaaac acuggcuagu ccacaaagag     1260

ugguuccacg acaucccauu gcccuggcac gccggagccg acaccgguac accccacugg     1320

aacaacaaag aagcccuagu agaauuuaaa gacgcccacg cuaaaagaca aaccgcaguc     1380

guccucggau cccaagaagg cgccguacac acagcacuag caggcgcgcu cgaagccgaa     1440

auggauggcg cgaaaggaag acucagcagc gggcaccuca aaugccgguu aaaaauggac     1500

aaacuccgcc ucaaaggcgu cucauacagc cucugcaccg cagcuuucac cuucacuaaa     1560

auccccgcgg aaacucuaca cgggacagug accgucgaag uccaauacgc cggaacagac     1620

ggcccaugca agguaccagc acaaauggca gucgacaugc aaacccuaac cccgguagga     1680

agauuaauaa cggccaaccc uguaauaacc gaaagcaccg aaaacagcaa gaugaugcuc     1740

gaacucgauc ccccuuuugg cgacagcuac aucguaaucg gcgucggcga aaagaaaauc     1800

acccaccacu ggcaccgauc cggauccaca aucggcaaag ccuucgaagc aacgguccgg     1860

ggagccaaac gaauggcggu ccugggagac acggcauggg acuucggauc cguagguggg     1920

gcccuaaacu cguuaggcaa aggcauccac cagaucuucg gcgcagccuu caaaucacuc     1980

uucgggggaa ugagcugguu cucgcaaauc uuaaucggaa cacuucuaau guggcucggc     2040

cuuaacacca aaaacggaag caucucgcug augugccuag cacuaggcgg aguccucaua     2100

uuccuaagca cagccguauc cgcagacgug ggcugcagcg uugacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5079
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 1 opt

<400>  5079
gggagaaagc uuaccaugag aaucauaaac gcacgcaagg aaaagaaacg gcgcggcgcc       60

gacacauccg uaggcaucgu cggcuuacuc cuaacaacag caauggcagc cgaaguaaca      120

aggcgaggaa gugccuacua cauguaccug gacaggaacg acgcaggaga agccaucagc      180

uucccaacca cucucggcau gaacaaaugc uauauacaaa ucauggaccu aggacauaug      240

ugcgacgcaa ccaugagcua cgaaugcccc augcuagacg aaggagugga accagacgau      300

guagacugcu ggugcaacac aaccucaaca ugggucguau acggaaccug ccaccacaag      360

aaaggugaag cccgacgcuc aagacgagcg guaacacucc cuagccacuc uacccgaaaa      420

cuccaaaccc gcagccaaac auggcucgaa ucccgagagu acacaaaaca ccuaauacga      480

guagaaaacu ggauauuccg aaacccggga uucgcccuag ccgcagccgc aaucgccugg      540

cuccuaggca gcagcaccuc gcaaaaaguc aucuaccuag ucaugauacu acucauagcc      600

cccgccuaca gcauccgcug cauaggagua ucgaaccggg acuucguaga gggcaugagc      660

ggcggcaccu gggucgacgu aguacuagaa cacggcgggu gcguuacggu cauggcacaa      720

gacaaaccca cggucgacau cgagcuaguc acgaccaccg ucucaaacau ggcagaaguc      780

cgcuccuacu guuacgaagc aucaaucagc gacauggcgu ccgacuccag gugcccgacc      840

caaggagaag ccuaucucga caagcaaagc gacacacagu auguuugcaa gcgcacccuu      900

gucgacaggg gcugggguaa cggcugcggc cucuucggga agggcucccu agucacaugu      960

gcaaaauucg caugcagcaa gaaaaugacc ggcaaaucca uccaaccaga aaaccucgaa     1020

uaccgcauaa uguuaagcgu acacggcucc caacauuccg gcaugaucgu uaacgacacc     1080

ggacacgaaa cagacgaaaa ccgagcaaaa gucgaaauaa caccaaacag cccccgagca     1140

gaagccaccc uaggaggauu cggaucacuc ggacuagacu gcgaaccgcg aaccggacuc     1200

gacuucucag accuuuacua ccuaacaaug aacaacaaac acuggcucgu acacaaagaa     1260

ugguuccacg acauaccacu gcccuggcac gccggcgcag acacaggaac gccacacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg ccaagcgaca aacaguggua     1380

guacucggaa cccaagaagg ggccguccac accgcccugg caggagcacu cgaagccgaa     1440

auggacgggg cgaagggacg ccucuccuca ggacacuuaa aaugcagacu uaagauggac     1500

aaacuccgac uaaaaggagu cagcuacagc cuaugcaccg cagcauucac guucaccaaa     1560

auaccagcug aaacacucca cggcacgguc acagucgaag uccaauacgc cggcacagac     1620

ggaccaugca aaguacccgc ccagauggcc guggacaugc aaacgcucac ccccgucgga     1680

cgccucauaa ccgccaaccc cgucauaacc gaauccaccg aaaacagcaa aaugaugcua     1740

gaacucgacc cucccuucgg agacucauac aucgucauag gcgucggcga aaagaaaauc     1800

acgcaccauu ggcacagauc cggaucaaca auaggcaaag cauucgaagc aacagugaga     1860

ggggccaagc gaauggcagu ccuuggagac acagcauggg acuucggaag cguagguggu     1920

gcgcuaaacu cccuaggcaa aggaauccac caaaucuucg gagcagcauu caaaagccuu     1980

uuuggcggaa ugucgugguu cagccaaauc cuaaucggga cgcugcuaau guggcuagga     2040

cuaaacacga aaaacggauc caucucgcug augugccuag cacuaggcgg aguccugauc     2100

uuccuuucaa ccgccgucuc cgcagacgua ggcugcucag ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5080
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 1 opt

<400>  5080
gggagaaagc uuaccaugcg aauaaucaac gcccgaaaag aaaagaaaag acgaggggca       60

gacacauccg ucggcauagu agggcuccuc cucacaacag ccauggccgc agaaguaacc      120

cgacgaggca gcgcauacua uauguaccuc gacaggaacg acgccgguga ggcaauaucg      180

uuccccacga cccucgggau gaacaagugc uauauacaga ucauggaccu cggccacaug      240

ugcgacgcaa caaugucaua cgaaugucca augcucgacg agggaguaga acccgaugac      300

guugacugcu gguguaacac caccagcacg uggguugucu acggaaccug ccaucacaag      360

aaaggcgaag cccgacgcuc ccgcagagcc gucacacugc ccucgcauuc aacaagaaaa      420

cuccaaacac gcucccaaac auggcucgaa ucccgcgaau auacaaaaca ccucauccgc      480

guagaaaacu ggauauucag gaaucccggg uucgcccuag ccgccgcagc aaucgccugg      540

uugcucggcu caagcaccag ccaaaaagua auauacuuag ucaugauccu acucaucgcc      600

cccgcguaca gcauccgcug caucggaguc aguaaccggg acuucgucga aggcaugucc      660

ggaggaacau ggguggacgu cguacuagag cacggaggcu gcgugaccgu aauggcacaa      720

gacaaaccca caguagauau agagcuagua acgacaaccg uauccaacau ggcagaaguu      780

cgaagcuacu gcuaugaagc aagcaucagc gacauggcaa gcgacucccg augccccacc      840

cagggagaag ccuaccuaga caagcaaucc gacacacaau acguaugcaa acgaacccuc      900

gucgaccgcg gauggggaaa cggcugcggc cuauucggga aaggaucccu aguaacaugc      960

gcaaaauucg caugcagcaa gaaaaugacc gguaagagca uccaacccga aaaccucgaa     1020

uaccgaauaa ugcucagugu acacggcucc caacacagcg gcaugaucgu aaacgacaca     1080

ggacacgaaa ccgacgaaaa ccgugcgaaa guggaaauaa ccccgaacuc ccccagagcc     1140

gaagcaaccc uaggcggcuu cggaucacug ggccucgacu gcgaaccaag aaccggacua     1200

gacuucuccg accuauacua ccucacaaug aacaacaaac acuggcucgu acacaaggaa     1260

ugguuccacg acauaccccu ccccuggcac gccggagcag acacagggac accgcacugg     1320

aacaauaaag aagcccuggu cgaguucaaa gacgcucacg caaaacgcca aacugucguc     1380

guccuuggca gccaagaagg cgccguccac acagcacucg ccggugcccu cgaagccgaa     1440

auggacggag ccaaagggcg gcuaagcagc gggcaccuga aaugccggcu uaaaauggac     1500

aaacuccgac ugaaaggugu aucauacagc cuaugcaccg cagccuucac auucacaaaa     1560

auacccgcag aaacacucca cggaaccguc acagucgaac uccaauacgc cggaaccgac     1620

ggacccugca aagugccagc ccaaauggca guagacaugc aaacgcucac ucccguaggc     1680

cgccuuauaa cugcgaaccc cgucauaacc gaaagcacag aaaacagcaa aaugaugcuc     1740

gaacucgauc ccccguuugg ggacucauac aucgucauug gcgugggcga aaagaaaauc     1800

acgcaccacu ggcaccgcuc aggcagcacu aucggaaaag cauucgaagc aaccguccgc     1860

ggcgccaaga gaauggcugu acucggcgac accgcauggg acuucggauc ggucggaggg     1920

gcccucaaca gccucggcaa aggaauacac caaaucuucg gcgccgccuu caaaucucua     1980

uucggaggga ugucgugguu uucccagauc cugauaggca cccuccucau guggcuaggc     2040

cucaacacga aaaacggcuc cauuagccuu augugccucg cccuaggcgg cguauuaauc     2100

uuccucucaa cagcagucag cgccgacgua gggugcagcg ucgacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5081
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 1 opt

<400>  5081
gggagaaagc uuaccaugcg gaucaucaac gcccgcaaag aaaagaagcg cagaggagca       60

gaaaccuccg ucggcauagu cggccugcua cuaaccaccg ccauggccgc cgaagucacc      120

agacgaggcu ccgccuacua cauguaccuc gacagaaacg acgccggcga agccaucucc      180

uucccuacaa cccucggcau gaacaaaugc uacauacaaa uaauggaccu aggccacaug      240

ugcgacgcca cuaugagcua cgaaugcccc augcucgacg aaggagucga acccgacgac      300

guggauugcu ggugcaacac cacaucgaca ugggucgugu acgggacgug ccaccacaaa      360

aagggagaag ccagacgauc acgcagagcc gucacacucc ccagccacag cacgagaaaa      420

cuacaaacac guagccaaac guggcucgaa agccgcgaau acaccaagca ccucauccgc      480

aucgagaacu ggauauuccg caaccccggc uucgcacuag ccgcggccgc caucgcaugg      540

cuacucggau cgagcaccuc acaaaaaguc auauaccuag ucaugauccu acuaauagcc      600

cccgcauacu ccauacgcug cauuggcguc agcaaccggg acuucguaga aggcaugagc      660

gguggcacau gggucgacgu cguccucgaa cacggaggcu gcguaaccgu cauggcucaa      720

gacaaaccua cgguagacau agaacugguu accacaacag ucagcaacau ggccgaaguc      780

cgcagcuauu gcuacgaagc aagcauauca gacauggcca gcgacucccg uugcccaaca      840

cagggagagg cauaccuaga caaacaaucc gauacacagu acguaugcaa acguacccuc      900

gucgaccgcg guuggggcaa uggcugcggc cuauucggaa aaggaagccu cgucacaugu      960

gcaaaauucg ccugcaguaa gaagaugaca ggcaaaucaa uccaacccga aaaccuagaa     1020

uacagaauaa ugcucucggu ccacggaagc caacacuccg gaaugaucgu aaacgacacc     1080

ggacacgaaa ccgacgagaa ccgcgccaag gucgaaauca cccccaacuc accacgcgcg     1140

gaagcaacac ugggaggcuu cggaucacuc ggacucgacu gcgaaccccg aaccggauua     1200

gacuuuuccg accucuacua ccucacaaug aacaauaaac acuggcucgu ccacaaggaa     1260

ugguuccaug acauaccacu acccuggcau gccggcgcgg auacgggaac gccccacugg     1320

aacaauaagg aggcccucgu ggaguucaaa gacgcccacg caaaacgcca aaccguagua     1380

gugcucggca gccaagaagg agcuguccac accgcccugg ccggagcccu cgaagccgaa     1440

auggacgggg cgaaaggacg ccucuccucg ggccaccuga aaugccgccu aaaaauggac     1500

aaacuccgau uaaaaggggu caguuacagc cuaugcaccg cggcauucac cuucacaaaa     1560

auaccagcag aaacacuaca uggaaccguc acagucgaag uccaguacgc cggcacagac     1620

gguccaugua aaguccccgc acaaauggcc gucgacaugc aaacacugac uccagucgga     1680

agacucauca ccgcaaaccc aguaauaacc gaaucaaccg aaaacuccaa aaugaugcuc     1740

gaacucgacc caccauucgg cgacuccuac auaguaauag gcgucggaga aaagaaaaua     1800

acacaccacu ggcaccgaag cggauccacc auuggcaagg cuuuugaagc aaccguacga     1860

ggagccaaac gcauggccgu gcuaggagac accgccuggg acuucggcuc cgucggcggc     1920

gcccuaaacu cccugggcaa aggcauacau caaauauucg gcgccgcauu caaaucacuc     1980

uucggaggca ugagcugguu cagccagauc cucauaggca cccuccuuau gugguugggc     2040

cucaacacca agaacggaag caucucccuc augugccuag cacucggcgg cguacucaua     2100

uuccucucaa cagccgucag cgccgacgua ggcugcagcg ucgacuucuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5082
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 1 opt

<400>  5082
gggagaaagc uuaccaugag aauaauaaac gcacgaaagg aaaagaaacg ccgaggcgcg       60

gacacaagcg ugggaauagu cggacuccua cucacaacgg caauggcagc cgaagucaca      120

cgaaggggcu cagcuuacua cauguaccug gacagaaacg acgccgggga agcaaucagc      180

uuuccgacca cacuaggaau gaauaaaugc uacauacaaa uaauggaccu cggacacaug      240

ugcgaugcaa ccaugucgua cgaaugccca augcuugacg agggcguaga gcccgacgac      300

gucgauugcu gguguaacac cacgagcaca uggguaguuu auggaacaug ccaccacaag      360

aaaggagaag cacgcagaag cagacgcgcu gucacgcucc caagucacag cacgcgcaag      420

cuccagaccc ggagccagac cugguuagaa agccgcgaau acaccaaaca ccucaucaga      480

gucgaaaacu ggaucuucag aaacccaggg uucgcacuag cagccgccgc cauagccugg      540

cuacucggau ccuccacgag ccagaaagua auauaccucg uaaugauacu ccugaucgca      600

ccagcauacu cgauccgcug cauaggcgua agcaaccggg acuucgugga agggauguca      660

ggaggaaccu gggucgaugu aguucuugag cacggaggau gugugacggu aauggcucag      720

gacaaaccca cgguagacau ugaacucguc accaccaccg ugagcaacau ggcagaagug      780

cgaucauacu gcuacgaggc cuccauaucc gacauggcca gugacucaag augcccaacg      840

caaggagaag cguaccuaga caaacaauca gacacgcagu acgucugcaa acgaacgcua      900

gucgaccgcg gaugggggaa cgggugcgga cuauuuggca aaggcucacu ggugacaugc      960

gccaaauuug caugcucaaa gaaaaugaca ggcaaaucca uacaacccga gaaccucgaa     1020

uaucggauca ugcuauccgu acauggcuca caacauagcg gcaugauagu caacgacaca     1080

ggacacgaaa cagacgagaa cagagccaaa gucgaaauaa ccccaaauag cccccgcgcg     1140

gaagcaaccc ucggaggauu cggaagccua ggacuggacu gugaaccaag aacaggcuug     1200

gacuuuagcg accucuacua ccugacaaug aacaacaaac acuggcuagu ccacaaagaa     1260

ugguuccacg acauaccgcu accguggcac gcaggggccg acaccggcac cccgcauugg     1320

aacaacaaag aggccuuagu cgaguucaaa gacgcacacg caaaaagaca aacgguagua     1380

guacucggcu cgcaagaagg cgccguacac accgcgcucg caggagcccu cgaagcggaa     1440

auggacggag caaaaggucg ccugagcucc ggccaucuca aaugcagacu aaaaauggac     1500

aaacugagac ucaaaggggu aucuuacagc cuaugcacgg cagccuucac auucacaaaa     1560

aucccugccg agacacuaca cgggacgguc acagucgagg uacaauacgc cggaaccgac     1620

ggaccaugca agguacccgc ccaaauggcc gucgacaugc aaacacuaac accagucggg     1680

cgccuaauca cagcuaaucc cgucaucacu gaaucaaucg aaaacagcaa aaugaugcua     1740

gaacuagauc cgcccuucgg cgacagcuac aucguaaucg gcgucggaga aaagaaaauc     1800

acacaccacu ggcauagaag cgggagcaca auaggcaaag cauucgaagc cacaguacgc     1860

ggagcgaaac gaauggcagu ccucggcgac accgcguggg auuucgggag cgucggcggc     1920

gcacucaacu cgcucggaaa aggaauccac caaaucuucg gagccgcauu caaaagccua     1980

uuuggcggca ugagcugguu uagccaaaua cucaucggaa cacuccugau guggcucgga     2040

cucaacacga aaaacggcag cauaaguuug augugccucg cccucggagg gguacucaua     2100

uuucugagca cagccgucag cgcagacgua gggugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5083
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 1 opt

<400>  5083
gggagaaagc uuaccaugcg caucauaaac gcccgcaaag aaaagaaacg cagaggcgca       60

gacacaucag ucggcaucgu aggacuucua cuaacuaccg cgauggcagc agagguaacc      120

cgccgcggau ccgcauauua cauguaccuc gaccgcaacg acgcgggcga agcaaucuca      180

uucccaacaa cccugggaau gaacaaaugu uacauccaaa ucauggaccu cggacacaug      240

ugcgacgcaa ccauguccua cgaaugcccc augcuagacg aaggcgucga accagacgac      300

gucgacugcu ggugcaacac caccagcaca uggguuguau acggcacgug ucaucacaag      360

aaaggagaag cgcgccgaag ccgacgcgca gugacgcugc ccucccacuc cacccgcaaa      420

cugcaaacgc gcucccagac auggcuagag agccgagagu acaccaaaca ccuaauccga      480

guagaaaacu ggaucuuccg caacccaggc uucgcacucg ccgccgccgc aaucgccugg      540

cuacucggau cuagcaccag ccaaaaagua aucuaccucg ucaugauccu ccugaucgcu      600

cccgccuaca gcauccggug caucgggguc agcaacagag acuucguaga aggaaugucc      660

gggggaacau ggguagacgu cguccuagaa cacgggggcu gcguaacugu cauggcucag      720

gacaaaccca cagucgacau agaacuuguc accaccaccg ucagcaauau ggcggaagua      780

cgcagcuacu guuacgaagc aagcauauca gacauggccu cugacagccg uugcccgacc      840

caaggagaag cauaucucga caaacagucc gacacccaau acgucugcaa acgaacccuc      900

guagaccgag gaugggggaa cggaugugga cucuucggca aaggaagccu cguaaccugc      960

gcaaaauucg cauguuccaa gaaaaugacc ggcaaaucaa uccaaccaga aaaccucgaa     1020

uaccgaauca ugcucagcgu ccacggaucc cagcacuccg gcaugaucgu aaacgauacu     1080

ggccacgaaa ccgacgaaaa ccgagccaaa gucgaaauaa cccccaauag cccacgcgcc     1140

gaagccaccc uaggcggauu cggcucccuc ggccucgacu gcgaaccacg caccggccua     1200

gacuucagcg accucuauua ccuaaccaug aacaacaaac acuggcuggu ccacaaagaa     1260

ugguuccacg acaucccccu cccuuggcac gccggggcgg acacaggaac cccacacugg     1320

aacaauaaag aagcacucgu cgaguucaaa gacgcacacg cuaaaaggca aaccgucgua     1380

guacuaggca gccaagaagg agcaguucac accgcauuag ccggcgcacu agaagccgaa     1440

auggacggag ucaaaggacg ccucuccucc ggacaccuaa aaugccgccu caaaauggac     1500

aaacuacgcc uaaaaggagu aaguuauuca cucugcacag cagccuucac auucaccaaa     1560

aucccagcag aaacccuaca cggcacagug acaguggaag uccaauacgc aggcaccgac     1620

ggcccaugca aagucccugc acagauggcc gucgacaugc aaacccucac accagucggc     1680

aggcucauca ccgcuaaccc aguaaucacc gaaucaaccg aaaacucaaa gaugaugcuc     1740

gaacucgacc cgcccuuugg ggacuccuac aucgugaucg ggguugggga aaagaaaauc     1800

acccaccacu ggcaccguuc aggaucaaca aucggcaaag cauucgaagc aaccguacga     1860

ggcgcaaaaa gaauggccgu acuaggcgac acagcauggg acuucggcuc agucggcggg     1920

gcccuaaacu cccucggcaa aggcauacac caaaucuucg gagcagcauu caagucccuc     1980

uucggcggca ugagcugguu cagccaaauc cucaucggca cacugcuaau guggcuggga     2040

cucaacacca aaaacggauc gaucucauuc augugccugg cacucggugg cgugcucaua     2100

uuccucagca cagccgucuc cgcagacguc ggcugcagcg uagauuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5084
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 1 opt

<400>  5084
gggagaaagc uuaccaugag gauaauaaac gcccgaaagg aaaagaaacg acgaggagcc       60

gacacauccg ucggcaucgu aggacuccuc cuuaccaccg ccauggcggc ggaggucaca      120

cgcagagggu cagccuacua cauguaccua gaccgcaacg acgccggcga agcgaucagu      180

uuucccacca cgcugggaau gaacaaaugc uacauccaaa ucauggaccu aggacacaug      240

ugcgacgcca caaugucaua cgaaugcccc augcucgacg aaggcgucga accagacgac      300

gucgacugcu ggugcaacac gaccuccaca ugggucgugu acggaaccug ucaccacaag      360

aaaggcgaag cccgacgauc acgcagagcc gucacccuac ccucccacag cacccgcaaa      420

cuucaaacca gaucacaaac cuggcucgaa ucccgagagu auacaaaaca ccucauacga      480

gucgaaaacu ggaucuuccg caacccagga uuugcccugg cggccgcugc cauagccugg      540

cuacucggcu ccucaaccuc acaaaagguc auauaccucg ucaugauccu ccuaaucgcc      600

cccgccuaca gcauccgcug uauaggaguc ucaaacagag acuuuguaga aggaauguca      660

ggcgggaccu ggguugacgu gguacuggaa cacggcggcu gcguaacugu cauggcgcaa      720

gacaaaccca caguggacau agagcuagua accaccaccg uaagcaacau ggcagaagua      780

cgcuccuacu gcuacgaagc aucgaucucc gacauggccu cagacucccg cugccccacc      840

caaggcgaag cauaccucga caaacaauca gacacccaau acgucugcaa gcgcacccuc      900

guagaccgcg gauggggcaa cggcugcggc cuguucggga aaggcucccu aguaaccugc      960

gccaaauucg caugcagcaa aaagaugacu ggcaaaucca uccaacccga gaaccucgaa     1020

uacagaauca ugcucucagu ccacggaucc caacacuccg gcaugauagu caaugacacg     1080

ggacacgaaa cagacgaaaa ccgcgcgaaa gucgagauca ccccaaacuc accccgagca     1140

gaagcaaccc uuggaggauu cggcagccua ggacucgacu gcgaacccag aaccggccua     1200

gacuucuccg accucuacua ucucaccaug aacaacaaac acugguuagu ccauaaggaa     1260

ugguuccacg acaucccccu ccccuggcac gcaggcgcug acaccggcac accccacugg     1320

aacaacaaag aggcacucgu cgaguucaag gacgcccacg caaaacgaca aacaguaguc     1380

guacucggcu cccaggaagg cgcaguacac acagcccuag caggcgcacu cgaagcggaa     1440

auggacggag caaaagguaa acuuucaagc ggccaccuca aaugccgacu gaaaauggac     1500

aagcugcgcc uaaaaggagu cagcuacagu cucugcacgg ccgcauucac auuuacaaaa     1560

auaccagccg aaacgcuaca cggcaccguc accgucgaag uccaauacgc cggcaccgac     1620

ggccccugca aaguacccgc acaaauggcc guagacaugc aaacccucac accgguagga     1680

cgccucauca ccgcaaaccc cgucauuaca gaauccaccg aaaacucaaa aaugaugcuc     1740

gaacuagauc ccccguuugg ugacuccuac aucgucaucg gaguaggaga aaagaaaaua     1800

acacaccacu ggcaccgcag cggaucgaca aucggaaaag ccuucgaggc aaccgugcgc     1860

ggcgcaaaac gaauggccgu ccucggagac accgccuggg acuucggcuc cgucggcggg     1920

gcacuaaaca gccuaggaaa aggaauccau cagauauucg gagcagccuu caaaucacug     1980

uucggaggca uguccugguu cucccaaaua cugauaggca cacuacucau gugguuaggc     2040

cucaacacca aaaacggaag cauaagccuc augugccucg cccucggcgg cguccucaua     2100

uuccucucca cagcggucuc cgcagacgug ggaugcagcg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5085
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 1 opt

<400>  5085
gggagaaagc uuaccaugcg aaucaucaac gcacgcaaag aaaaaaaacg gcgaggcgcc       60

gacaccagcg uaggcauagu aggacuccua cuaacaacag ccauggccgc cgaaguaacc      120

cgacgcggcu ccgccuacua cauguaucuc gaccggaacg acgccggcga agcaauaucc      180

uuccccacaa cccucggcau gaacaaaugc uacauacaaa ucauggaccu cggccauaug      240

ugcgacgcaa caaugucaua cgaaugcccc augcucgacg aaggcguuga gccagacgac      300

gucgacuguu ggugcaacac cacaagcacc uggguagucu acggaacaug ccaccauaaa      360

aaaggcgaag ccagacgauc caggagagcg guaacucugc caucacacuc cacacgaaaa      420

cuacaaacca gaucccaaac auggcucgaa agccgcgaau acaccaagca ccucauccgc      480

guagagaacu ggauuuuccg aaaccccgga uucgcccuag ccgccgccgc caucgccugg      540

cuucugggau ccucgaccag ccaaaaagua aucuaccucg ucaugauccu cuuaauagcc      600

cccgcguaca gcauacgaug caucggagua ucgaaccgcg acuucguaga gggaaugagc      660

ggcggcacau ggguagacgu aguacucgag cacggagguu gcguaacagu cauggcacaa      720

gacaaaccua caguggacau cgaacucgua acgaccaccg ucagcaacau ggcggaagug      780

cgcucauacu gcuacgaagc aucgauaagc gacauggccu ccgacucccg cugcccaacg      840

caaggcgaag ccuaccucga uaaacagucc gacacccagu acgucugcaa acgcaccuua      900

gucgacaggg gauggggaaa cggcugcggc cuauucggca aaggcagccu ugucaccugc      960

gccaaauucg cgugcagcaa aaaaaugaca ggaaaaucca uccaacccga aaacuuagag     1020

uaccgcauaa uguuaucagu ccacggaucc caacacucag gaaugaucgu caacgauaca     1080

ggccacgaaa cagacgaaaa ccgcgccaaa gucgaaauca cacccaacuc cccccgcgca     1140

gaagccaccc uaggcggguu cggaagccuc ggacuagacu gcgagccccg cacaggacuu     1200

gacuucagug accuauacua ccuaacaaug aacaacaagc acuggcucgu ccacaaagaa     1260

ugguuccacg acaucccccu acccuggcac gcaggagccg auaccggcac accucacugg     1320

aauaacaaag aagcgcuagu cgaauucaaa gaagcacacg caaaacgaca aacaguagua     1380

guguuaggcu cacaagaagg cgcaguccac accgcgcuag ccggagcccu agaagcggaa     1440

auggacggcg caaaaggccg ccuaucaagc ggccaucuaa agugucgacu aaaaauggac     1500

aaacuccgac ugaaaggcgu auccuauagc cugugcaccg ccgcauucac cuucacaaaa     1560

auccccgcag agacccuuca cggaacgguc acuguagaag uccaguacgc aggcaccgac     1620

ggaccaugca aaguccccgc acaaauggcc guugacaugc aaacccuaac accagucgga     1680

agacucauaa ccgccaaccc agucaucaca gaaaguaccg aaaacagcaa aaugaugcuc     1740

gaacucgacc ccccguucgg cgacagcuac aucgugaucg gcguaggcga aaaaaaaaua     1800

acgcaccacu ggcauagauc aggcagcacc auaggaaaag ccuucgaagc aacaguacgc     1860

ggcgcaaaac gaauggccgu acucggcgac acggcauggg acuucggcuc cgugggcgga     1920

gcgcugaaua gucugggcaa agguauccac caaaucuuug gggccgcauu uaaaucccuc     1980

uucggcggca ugucaugguu cucacaaauc cuaauaggaa cacuucugau guggcuggga     2040

cucaacacga aaaacggcuc caucucccug augugccuag cacucggcgg cguacugaua     2100

uuccuaucca cagcagucuc agcagacgua ggcugcucgg ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5086
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 1 opt

<400>  5086
gggagaaagc uuaccaugcg aaucaucaac gcaaggaaag aaaagaaaag acggggcgcg       60

gacacaagcg ucgggaucgu cggucuacua cucacaaccg caauggccgc ugaagucacc      120

cgcagaggca gcgcauauua cauguaccug gacagaaaug acgcaggaga ggcaaucagc      180

uuccccacaa cgcuaggcau gaacaaaugc uacauacaaa ucauggaccu aggacacaug      240

ugcgaugcga caaugagcua ugagugcccc augcucgacg aaggagucga accagacgau      300

gucgacugcu ggugcaacac cacaagcacg ugggucguau acggcacaug ccaccacaaa      360

aagggcgaag cacgacgaag cagacgagcc gucacacugc caucacacuc cacacgcaaa      420

cuccaaacac ggagccaaac auggcuggaa ucccgggaau acaccaaaca ccucauccgg      480

gucgaaaacu ggauauucag aaacccagga uucgcccucg cagcugccgc cauagcaugg      540

cuccuaggcu caagcacauc acaaaaagug auauaccuug uaaugauacu acucaucgcg      600

cccgccuacu caauacgaug cauaggggua agcaaucgcg auuucgugga agguauguca      660

gguggcaccu ggguagacgu cgugcucgag cacggaggau guguaacggu aauggcacag      720

gacaaaccca cggucgacau cgagcucguc accacgacgg uaagcaacau ggccgaagua      780

cgcucauacu gcuacgaggc guccauauca gacauggcau cagacagccg cugccccacc      840

cagggagagg cauaccugga caaacaaagu gauacacaau acguaugcaa acggacgcuc      900

gucgacagag gcuggggcaa cggaugugga cuauucggca aaggaagccu aguaacgugc      960

gccaaauucg ccugcagcaa gaaaaugaca ggaaaaucca uacaaccaga aaaccuagaa     1020

uaccgaauaa ugcucucagu acacggcuca caacacagug gcaugauagu aaacgacacc     1080

ggccaugaaa cugacgaaaa ccgcgccaaa gucgaaauca cccccaacag cccgcgagca     1140

gaagcaacac ucggaggauu cggauccuua ggacucgacu gcgaaccacg cacaggacuc     1200

gauuucagcg accucuacua ccucacgaug aacaauaaac auuggcuagu ccacaaagaa     1260

ugguuccacg acaucccccu acccuggcac gcaggcgccg acacaggaac accccacugg     1320

aacaacaaag aagcacuggu agaguucaaa gacgcacacg ccaaacgcca gacagucgug     1380

guccuaggau cacaggaggg ggcaguccac accgcacucg ccggagcccu agaagccgag     1440

auggacggag ccaaaggaag acucagcucg ggccaccuca aaugcagacu aaaaauggau     1500

aaacuaagac ucaaaggugu cuccuacucc cucuguacag ccgccuucac auucaccaaa     1560

auaccagccg agacgcuaca cggcaccgua acagucgagg ugcaguacgc aggcgcggac     1620

ggaccaugca aggugccagc ccaaauggcc guagacaugc agacccucac accgguaggc     1680

cgacuaauaa ccgccaaucc cguaauaacc gaauccaccg aaaacucaaa aaugauguua     1740

gaacuggacc cgcccuucgg ggacagcuac auagucaucg gagugggaga aaagaaaauc     1800

acacaccacu ggcacagaag uggaagcaca auagggaaag cguuugaggc caccguacgc     1860

ggcgccaaac gcauggcagu ccuaggagac accgcauggg acuucggcag cgucggcgga     1920

gcacucaacu cacugggcaa gggaauccac caaauuuucg gcgcagcauu caaaagccua     1980

uucggaggaa ugagcugguu cucacaaaua cugaucggca cacuacuaau guggcuggga     2040

cugaauacca aaaacggaag caucaguuua augugccuug cacuaggagg cguacugaua     2100

uuccuaagca cagccgucuc cgcagacgug gggugcagcg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5087
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 1 opt

<400>  5087
gggagaaagc uuaccaugag aauaauaaac gcagggaaag agaagaaacg aaggggagcc       60

gacacaaacg ucggcaucgu cggacuauug cuaacaacgg cgauggcagc cgaaguaaca      120

cgccgcggau cagcguacua cauguaccug gaccgaaacg acgccggaga agcaaucucc      180

uucccaacca cgcucggcau gaacaaaugc uacauccaaa uaauggaccu aggacacaug      240

ugcgacgcca ccauguccua cgaaugcccg augcucgacg aaggcgucga accagacgau      300

gucgauugcu gguguaauac cacgaguaca ugggucgucu acggcacaug ccaucacaag      360

aaaggagaag cacgcagaag cagacgcgcc guaacacuac caagccauuc cacaagaaaa      420

cuacagacaa ggagccaaac auggcuggaa agccgcgaau acacaaaaca ccuaaucaga      480

guggaaaacu ggaucuucag gaauccagga uucgcacugg ccgcggcggc aauagcgugg      540

cuccuaggaa gcaguacauc acaaaaagua aucuaccucg uaaugauauu gcugaucgcg      600

ccagccuacu cuauaaggug cauaggaguc agcaaccgug acuucguaga aggaaugucc      660

ggcggcaccu gggucgacgu cgugcucgaa cacggagggu gugucaccgu aauggcccaa      720

gacaaaccca ccguagacau agaacugguc acaacgaccg uaagcaacau ggcagaagug      780

cgcagcuacu gcuacgaggc cucaaucagc gacauggcaa gcgacucccg cugcccaaca      840

caaggcgaag cauaccugga caaacagagc gacacccagu augucugcaa aaggacccuc      900

gucgaccgag gauggggaaa cggaugcggc cuauucggca aaggaucccu agugacgugc      960

gccaaauucg caugcaguaa gaaaaugacc ggcaaaagca uacaaccaga aaacuuagaa     1020

uacaggauca ugcucucagu gcacggauca caacacagcg gaaugauagu caacgacacc     1080

ggccacgaaa cggacgaaaa cagagccaaa gucgaaauua cacccaacag uccacgcgca     1140

gaagcgaccc ucggaggguu cgggagcuua ggacucgacu gcgaaccacg aacgggacuc     1200

gacuucagcg accuguauua ccuaacaaug aacaacaaac auuggcuagu gcacaaagag     1260

ugguuccacg acaucccccu accauggcac gccggggccg acacaggcac accccauugg     1320

aacaacaaag aagcucuagu cgaguucaaa gacgcacacg caaaaaggca aaccgucgug     1380

guacuuggca gccaagaagg cgcaguccac acugcacuag caggagcgcu agaagccgaa     1440

auggacggag caaaaggccg ccuauccucg gggcaccuca aaugcagauu gaaaauggac     1500

aaacuaagac ugaaaggagu aucauacucc cucugcaccg cugccuucac cuucaccaaa     1560

auacccgcag aaacccucca cggaacggua acagucgaag uacaguaugc cggaaccgac     1620

ggccccugca aggucccagc ccaaauggcc gucgacaugc aaacacuaac cccaguuggc     1680

cgccuaauca cggccaaccc aguaaucaca gaaucaaccg agaacuccaa aaugaugcuc     1740

gaacucgacc cacccuucgg agacagcuac aucguaaucg gcgucggcga aaagaaaaua     1800

acccaccacu ggcaccgcag cggaagcaca auuggcaaag cguucgaagc aacggucaga     1860

ggcgcacgca gaauggcagu ucucggcgau acagcauggg auuucggcag cguaggaggg     1920

gcgcucaacu cccucgggaa aggcauacau caaaucuuug gggcagcguu caaaagccug     1980

uucggcggca ugucaugguu cagucaaauc cuaauaggaa cacuccuaau guggcucggg     2040

cuaaacacga aaaacggguc caucagcuua augugccucg cacucggagg aguacucauc     2100

uuccuaucca cagcuguaag cgcagacguc ggaugcucag uggacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5088
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 1 opt

<400>  5088
gggagaaagc uuaccaugcg cauaaucaac gccagaaaag agaagaaacg acgaggagca       60

gaaaccagcg uggggauagu gggccugcuc cuaaccacag caauggcugc cgaagugaca      120

cgaagaggaa gcgccuauua cauguaccug gacagaaaag acgcaggaga agccaucagc      180

uuuccaacca cacucgggau gaacaaaugc uacauccaga uaauggaccu aggccacaug      240

ugcgacgcaa ccaugucaua cgaaugcccc auguuagacg aaggcgugga gccggacgac      300

guagacugcu ggugcaacac caccagcacu uggguggugu acggaacaug ccaccacaag      360

aaaggcgaag ccagacgcag cagacgagcc gucacgcucc ccagccacuc cacacggaaa      420

cuacagacac gaagccaaac auggcuagaa agccgcgaau acaccaaaca ccuaaucaga      480

gucgagaacu ggaucuuccg caaucccggc uucgcauuag ccgcagccgc aauagccugg      540

cuacuaggca gcagcacauc ccaaaaaguu aucuaucuug ugaugauacu gcuaauagcc      600

cccgcguacu caauccggug caucggcguc ucaaaccggg acuucguaga aggcauguca      660

ggcggcaccu gggucgacgu cguacuagaa cauggaggau gcgucaccgu aauggcccaa      720

gacaaaccca ccguagacau cgaacucguc acgacuaccg ucaguaacau ggcagaagug      780

cgauccuacu gcuacgaagc cagcaucucc gacauggccu ccgacagccg cugcccaaca      840

caaggcgaag cauaccucga caaacaaagc gacacacaau acgugugcaa acgaacccua      900

gucgaccgcg gaugggguaa cggaugcggc cucuucggaa agggcagccu agugaccugc      960

gcaaaguucg caugcagcaa gaaaaugaca ggcaaaagua uccagccaga aaaucuggag     1020

uacagaauaa ugcuuucagu acacggauca caacacucag gaaugaucgu caacgacaca     1080

ggccaugaaa cagacgagaa ccgcgcgaaa guagaaauaa cccccaacag ccccagagca     1140

gaagcaacac ucggaggguu cggcagccua ggacucgacu gcgaaccacg aaccgggcua     1200

gacuucagcg accuauacua cuuaacaaug aacaauaaac acuggcucgu ucacaaagaa     1260

ugguuccacg acaucccccu accauggcau gcaggagccg acaccggaac gccccacugg     1320

aacaacaagg aggccuuagu agaguucaag gacgcacacg cgaagcgaca aaccguaguc     1380

guccuaggau cccaagaagg agccguacac acagcacugg ccggcgcccu cgaagcagaa     1440

auggacgggg caaaaggccg acuaagcagc ggccaccuaa agugcagacu gaagauggac     1500

aaacugagac ucaaaggcgu cuccuacagc cucugcaccg ccgcauucac auucaccaag     1560

auaccagcgg aaacacuaca cggaaccguc accguagaag ugcaauacgc aggcacugac     1620

ggccccugca aagucccagc acaaauggca guagacaugc aaacacucac accaguagga     1680

cgacucauca ccgccaaccc aguuaucacc gagucaacag aaaacucaaa aaugaugcuc     1740

gaacucgacc cgcccuucgg cgacagcuac aucguaauag gagugggaga aaagaaaaua     1800

acgcaccacu ggcaccggag cggcagcaca auaggaaaag cauucgaagc aacagucaga     1860

ggcgccaaaa gaauggccgu ccucggcgac acggcauggg acuucgggag cgucggaggc     1920

gcauuaaacu cccuggggaa aggaauccac caaauuuucg gagccgccuu caaaagccua     1980

uucggcggga ugucaugguu cagccaaauc cucauuggga cccuacuaau guggcuaggc     2040

cugaacacga agaacggaag cauaucacua augugccuag cacuaggggg aguccuaaua     2100

uuccuaucca cggcgguaag cgcagacgua ggaugcuccg uagacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5089
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 1 opt

<400>  5089
gggagaaagc uuaccaugcg aaucaucaac gcgagaaaag aaaagaaaag acguggagcg       60

gacacguccg ucggaaucgu cggacuauug cuaacgacgg cuauggccgc ggaagucaca      120

cgcagaggca gcgccuacua cauguaccuc gaccgcaacg acgcuggcga agcgaucagc      180

uuccccacca cccuuggaau gaacaagugu uacauccaga uaauggacuu aggacacaug      240

ugcgacgcca ccauguccua cgaaugcccu augcuugacg aaggcgucga gccagacgac      300

guggauugcu ggugcaacac aaccagcaca uggguaguau acggaaccug ccaucacaag      360

aaaggcgaag cccgaaggag uagacgagcc guaaccuuac caucgcacuc aacacgaaaa      420

cuacaaaccc gcucccaaac auggcuagaa ucccgcgaau acacaaaaca ccucauccgc      480

gucgaaaacu ggauauuccg caaccccgga uucgcacuag ccgccgccgc aaucgccugg      540

cuacucggaa gcucaaccuc gcaaaaaguc auauaccucg uaaugauacu gcuaauagca      600

ccggcauacu ccauacgaug uaucggcgug uccaacaggg acuucgucga aggaaugucc      660

ggaggaaccu gggucgacgu gguacuagag cacggaggcu gcgucacagu gauggcccag      720

gacaaaccaa cagucgacau agagcuaguc acaacaaccg uguccaacau ggccgaagua      780

agguccuacu gcuacgaagc cagcauaagc gacaugguau cagauucgcg augccccacc      840

caaggcgaag cauaccucga caaacaaagc gacacccagu acguaugcaa acgcacgcuc      900

gucgacagag gauggggcaa cggaugcggu uuauucggca aaggaagccu aguaaccugc      960

gcaaaauucg ccugcaguaa gaaaaugacc ggaaaaucca uacaaccaga aaaccuagaa     1020

uaccgcauca ugcucuccgu ccacggauca caacacagcg gaaugauagu caacgacacg     1080

ggacacgaaa ccgacgaaaa cagggccaaa guagaaauaa ccccaaacag uccccgugca     1140

gaagcaacac ucggcggguu cggcucacuc ggccucgacu gcgaaccaag aaccggccua     1200

gacuucuccg accucuacua ccuaacaaug aacaacaaac acuggcucgu acacaaagaa     1260

ugguuccacg acauaccccu accauggcac gccggcgccg acaccggcac uccacacugg     1320

aacaacaagg aagcccucgu agaguucaaa gacgcacacg cuaaacgcca aaccgucgua     1380

guccucggaa gccaggaagg agccguccac accgcccugg caggggcccu ggaagcugaa     1440

auggacggag caaaaggcag acucucaagc ggccaccuaa agugccgacu caaaauggac     1500

aaacucagac uaaagggagu aagcuacagc cucugcacag ccgcauucac auucaccaaa     1560

aucccagccg aaacucuaca cggcacaguc accguagaag uacaauacgc cggaacagac     1620

ggaccgugua aaguaccagc ucaaauggcc guggacaugc aaacacugac acccgucgga     1680

agacucauaa ccgccaaccc agucauaacc gaaucgaccg aaaacagcaa gaugaugcua     1740

gaacuagacc cgcccuucgg agacagcuac aucguaaucg gcguagggga aaagaaaaua     1800

acccaccacu ggcacagauc aggcuccaca auaggaaaag ccuucgaagc cacaguacga     1860

ggcgccaaac gaauggcugu auuaggugac acggcauggg acuuuggauc aguaggcggc     1920

gcccuaaacu cacuaggaaa aggcauccac caaaucuucg gggcagccuu caagucgcuc     1980

uuugggggca uguccugguu cucgcaaauc cucaucggga cccuccuaau guggcuagga     2040

cucaacacca agaacgguuc gauaucccug augugccucg cucucggagg aguccucauc     2100

uuccucucca cugccgucag cgccgacguu ggcugcucgg ucgauuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5090
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 1 opt

<400>  5090
gggagaaagc uuaccaugag aaucauaaac gcaagaaaag aaaagaaaag aagaggcgcg       60

gauaccaaug ucggaaucgu gggacuccua cuaacaacgg caauggcagc ggaaguaacc      120

cgacgaggca gcgcauacua cauguaccuc gacagaaacg acgcaggaga agcaaucagc      180

uuccccacca cucuaggcau gaacaaaugc uacauccaaa uaauggaccu cgggcacaug      240

ugcgacgcga caauguccua cgaaugcccc augcuggacg aaggcguaga acccgacgac      300

gucgacugcu ggugcaacau gacgagcaca uggguaguau acgggacgug ccaccacaag      360

aaaggcgaag cacgacgaag ccgccgggca guaacccucc caagccacag cacgcgcaaa      420

cuacagacuc gcucacaaac cuggcucgag agccgagaau acacaaagca ccuaauccgc      480

guagaaaacu ggaucuuccg aaaccccggc uucgcacuag cagcagcagc gauagcgugg      540

cuacugggcu caagcaccag ucagaaaguc aucuaccugg uaaugauacu acuaauagca      600

ccagccuaca gcaucaggug cauaggcgua agcaaccgag acuucguaga aggaaugagc      660

ggaggcaccu ggguggacgu cguucucgaa cacggagggu gcgucacagu aauggcccaa      720

gacaaaccca caguggacau cgagcuagua acaaccacag ucagcaacau ggcagaaguc      780

cgcucauacu gcuacgaagc cuccaucucc gacauggcca gugacucacg cugcccaaca      840

caaggagaag ccuaccucga caaacaauca gacacacagu acgucugcaa acgcacacug      900

gucgaccgcg gauggggaaa ugggugcgga cuauucggaa aaggaucccu cguaaccugc      960

gccaaauucg ccugcaguaa gaaaaugaca ggcaaaucaa uccagccgga aaaccuagaa     1020

uaccgcauca ugcucagcgu ccacggcagc caacacagcg guaugauagu aaacgacaca     1080

ggacacgaaa cagaugaaaa ccgcgcaaaa gucgagauca cacccaacag cccccgcgcc     1140

gaagcaaccc uagguggguu uggcagccug ggacuagacu gcgaaccacg aaccggacuc     1200

gacuucuccg accuauacua ccuaacuaug aacaauaaac acuggcucgu acauaaggaa     1260

ugguuccacg acaucccacu accauggcac gcuggagccg acaccggaac cccgcacugg     1320

aacaacaaag aagcgcucgu agaguucaaa gacgcacacg ccaaacgaca aacagucguu     1380

guacucggua gccaggaagg agcaguacac acagcacucg ccggagcacu agaagcagaa     1440

auggacggag caaaaggacg acugagcagc ggccaccuga agugcagacu caaaauggac     1500

aagcuccgcc ucaaaggagu cagcuacagc cucugcaccg ccgcguucac cuucacaaaa     1560

auaccagcgg aaacacuuca cggaacggua acaguggaag uacaauacgc cggaacagac     1620

ggacccugca aagucccagc ccagauggcc gucgacaugc aaaccuuaac cccagucggc     1680

cgccuaauca ccgcaaaccc cgucaucacc gaauccaccg aaaacuccaa aaugaugcug     1740

gaacuagacc cacccuuugg agacuccuac aucguuauag gaguaggcga gaaaaagauc     1800

acccaccacu ggcaccgcuc cggaagcacg aucggcaaag ccuucgaagc caccguccgg     1860

ggagccaaac gcauggcagu acuaggagac acagcauggg acuucggaag cgugggcggg     1920

gcccugaaca gccuaggcaa aggaauccac cagauauucg gcgccgccuu caaaagccua     1980

uucggcggca uguccugguu cucgcaaaua cuuaucggca cacuauuaau guggcucgga     2040

cucaacacaa agaacggcuc aaucagccua augugucucg cucugggagg aguccuaaua     2100

uuccucagca cggcagucag cgcggacguc ggcugcucag ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5091
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 1 opt

<400>  5091
gggagaaagc uuaccaugcg cauaaucaac gcccgaaaag agaaaaagcg acgcggggcc       60

gacacgagcg ucgguauagu aggccuacuu cugaccacag ccauggcggc cgaagucacg      120

cgccggggca gcgccuacua cauguaccua gaccgaaacg acgcaggaga agcaaucagc      180

uucccaacca cgcuaggcau gaacaaaugc uacauacaaa ucauggaccu aggucacaug      240

ugcgacgcaa ccaugagcua cgaaugcccu augcuagacg aaggagucga acccgacgac      300

guggacugcu ggugcaacac cacaagcaca ugggucgucu acggaaccug ccaucacaaa      360

aagggcgaag cacgcagaag ccgacgcgcc gucaccuugc caagccacag cacccggaaa      420

cugcaaacaa gaagccaaac guggcuagaa agucgcgaau auaccaaaca ucucauacgc      480

gucgaaaacu ggaucuuccg aaaccccggc uucgcacucg cagcggccgc aauagcaugg      540

cuccuagggu ccagcacaag ccaaaaaguu aucuaccucg uaaugauccu acucaucgcu      600

ccagcauacu ccauacgaug caucggcguc agcaaccgag auuucgucga aggaaugucc      660

ggaggcaccu ggguagacgu cguacucgag cacggcggcu gcgucaccgu cauggcccaa      720

gacaaaccaa cugucgacau agaacucguc accaccaccg ucucaaacau ggccgaagug      780

cgcagcuacu gcuacgaagc gagcaucagc gacauggcca gcgacuccag augucccacu      840

caaggagaag ccuaccucga caaacaaucc gacacccaau acgugugcaa acgcacgcua      900

gucgaccgag gauggggaaa uggaugcgga cucuuuggaa aagguucguu aguaaccugc      960

gccaaauucg caugcuccaa aaagaugaca ggcaaaucaa uacagccgga aaaccucgaa     1020

uacagaauca ugcucagcgu ccacggcagc caacauagcg gaaugaucgu aaacgacacg     1080

ggacacgaaa cagaugaaaa uagggcaaaa gucgagauca ccccuaacag uccccgagcc     1140

gaggcggcac uaggcggauu cggaucacua ggacucgacu gcgagccccg cacuggccug     1200

gacuucuccg accucuacua ccucaccaug aacaacaaac acuggcuagu gcacaaggaa     1260

ugguuccacg acaucccccu acccuggcac gccggcgccg acaccggaac uccccauugg     1320

aacaacaaag aagcacuggu cgaauuuaaa gacgcacacg cgaaacgaca aacagucgua     1380

guacucggau cccaagaagg ugccguccac acagcacuag ccggagcgcu ggaggcagag     1440

auggauggcg ccaagggccg gcucuccucc ggccaccuaa agugucgucu aaaaauggac     1500

aaacuccgcc uaaaaggcgu gucguacagc cuaugcaccg cagccuucac cuucaccaaa     1560

auccccgccg agacacuaca cggaaccguc acagucgaag uccaauacgc cggcacagac     1620

ggcccgugca aagugccggc ccaaauggca guggacaugc agacccuaac accagucgga     1680

cggcuaauca cagccaaucc cguaaucaca gaaagcacag aaaacagcaa aaugauguua     1740

gaacuagauc ccccauucgg cgacagcuac auagucauag gcgucggaga gaagaaaaua     1800

acacaccacu ggcacagaag cggcagcaca aucggaaaag cauuugaagc aaccguccgc     1860

ggagcaaaaa gaauggcagu acugggcgac accgcauggg acuucggauc aguaggcggc     1920

gcacuaaacu cauuaggcaa agggauacac caaauauucg gagcugcauu caaaagccuc     1980

uucgggggca ugagcugguu cucacaaaca cucaucggca cccuacugau guggcuggga     2040

cuaaauacca aaaacggaag caucucgcuc augugccucg ccuuaggagg aguccuaauc     2100

uuccucagca ccgcuguaag cgccgacguc ggcugcagcg ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5092
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 1 opt

<400>  5092
gggagaaagc uuaccaugcg cauaaucaau gccaggaaag aaaagaaaag gcgcggcgca       60

gaaaccucag ugggaaucgu cggacuacua cugaccacag caauggccgc cgaggugaca      120

cgacgcggau cugccuacua cauguaccua gaccgaaacg acgcuggcga agcgaucucc      180

uuccccacaa cccucggaau gaauaaaugc uacauacaaa uaauggaccu aggccacaug      240

ugugacgcca caauguccua cgaaugccca augcucgacg aaggugugga acccgacgac      300

guggacugcu ggugcaacac aacgucaacg uggguaguau acggaaccug ccaccacaag      360

aaaggagaag caagaagaag ccgacgagcu guaacacucc cgagccacag cacgcgaaaa      420

cuccaaacca gaagccaaac guggcuagaa agcagagaau acaccaaaca ccuaauacgc      480

gucgagaacu ggauauuucg aaaccccgga uucgcacucg cagccgccgc aauagccugg      540

cuacuaggca guucaaccag ucaaaaaguc aucuaccuag uuaugauacu acucaucgca      600

cuagcauaua gcauccgaug uauagggguc ucaaaccgcg acuucguaga gggaaugagc      660

ggcggcaccu ggguagacgu cguccucgaa cacggcggau gcguuaccgu aauggcgcag      720

gacaaaccca ccguggacau agagcuaguu accacgacag uaagcaacau ggcggaaguc      780

agaucauacu gcuacgaagc aagcaucagc gacauggcaa gcgacagcag augcccaacg      840

caaggagaag ccuaccucga caaacagagc gacacacaau acgucugcaa acgcacgcuu      900

guagaccgag gauggggcaa cggaugcgga cuguucggaa aaggcucauu agucacaugc      960

gccaaauucg cgugcagcaa gaaaaugacc ggaaaaagua uccaacccga aaaccucgaa     1020

uaccgaauca ugcucuccgu ccacggauca cagcacagcg gaaugauagu aaacgacaca     1080

ggacacgaaa ccgaugaaaa ccgggcgaaa guagaaauca cuccaaacuc cccuagagcg     1140

gaagccacac uaggcggcuu cggaagccua ggccucgacu gcgaaccacg cacgggccuc     1200

gacuucagcg accucuacua ccuuaccaug aacaacaaac acuggcuggu acacaaagaa     1260

ugguuccacg acaucccccu accauggcac gcaggagcag acacaggcac accccacugg     1320

aacaauaaag aggcacucgu cgaguucaaa gacgcccacg caaaacgaca aaccgucguc     1380

guccuaggaa gucaagaagg cgcgguccac acggcacucg ccggugcgcu agaagcagag     1440

auggacggcg cgaaaggacg acuaagcucc ggacaucuca aaugcaggcu caaaauggac     1500

aaacuccggc ugaaaggagu aagcuacucg cucugcaccg cugccuucac auucaccaaa     1560

auccccgccg aaacacucca cggcaccguc accgucgagg uacaauacgc aggcacagau     1620

ggaccgugca aagucccagc acaaauggcc guagacaugc aaacacugac acccgucgga     1680

cgacucauca ccgcuaaccc cgucaucacc gaaaguaccg agaacagcaa aaugaugcua     1740

gaacucgacc cgcccuucgg agacucauac aucguaaucg gagucgggga aaagaaaaua     1800

acacaccauu ggcaccgauc cggcuccacu auaggaaaag cguucgaagc cacgguacgc     1860

ggugccaaac gaauggcagu ccucggcgac accgccuggg acuucggaag ugucggaggc     1920

gcauuaaacu cccucggcaa aggaauacac caaaucuucg gcgccgcauu caaaagccua     1980

uucggaggaa uguccugguu cucccaaaua cugauaggga cacuccucau guggcucggu     2040

cuaaacacca aaaacggaag caucucccuc augugccuug cccuuggagg aguacucauc     2100

uucuugucaa cagccguaag ugcugacguc ggcugcagcg ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5093
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 1 opt

<400>  5093
gggagaaagc uuaccaugcg aaucauaaac gcuaggaaag agaagaaacg gcgaggagcg       60

gacacauccg ugggaauagu uggccugcuc cuaacaaccg caauggcggc agaaguaacg      120

cgccgaggcu ccgccuacua cauguaccua gaccguaacg augcugggga ggcaauaucc      180

uucccuacca cccucggcau gaacaaaugc uacauccaaa uaauggaccu cggacacaug      240

ugcgacgcca caauguccua cgagugcccg augcuagacg aaggcgucga accagacgac      300

gucgacugcu ggugcaauac aaccagcacc ugggucguau acggcacuug ccaccacaag      360

aaaggagaag ccagacgcag cagacgagcc gucacgcucc caagccacuc cacccgcaaa      420

cuacagaccc gaucccaaac auggcucgaa agccgagaau acacaaaaca cuuaauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcacucg cagcggccgc cauagccugg      540

cuccucggga gcagcaccuc ccagaaggua aucuaccugg ugaugauacu ccuaauugca      600

cccgccuacu ccauacggug cauaggcaua uccaaccgag acuucgucga aggcauguca      660

ggaggaaccu gggucgacgu cguucuagaa cacggaggcu gcguaaccgu cauggcccaa      720

gacaaaccaa cagucgacau agagcuaguc accacaaccg ucuccaacau ggccgaaguc      780

agaaguuacu gcuacgaggc aagcaucagc gacauggcca gcgacagccg cugccccaca      840

caaggagaag cauaccucga uaaacaauca gacacccaau acgucugcaa acgcacacug      900

guagacagag gauggggcaa cggcugcggu cucuucggga aaggcucacu gguaaccugc      960

gccaaauucg ccugcuccaa gaaaaugaca gggaaaucca uacaaccaga aaaccuagaa     1020

uaccgcauaa ugcuaucugu ccacggauca caacacuccg gcaugauagu aaacgacacc     1080

gggcacgaaa ccgacgaaaa cagagccaaa guagaaauca cccccaacag cccccgcgcg     1140

gaagcaacac ucggaggauu cggcauacua ggacuggacu gcgaacccag aaccggacug     1200

gauuucagcg accuuuauua ccucacaaug aacaauaaac acuggcuggu ccauaaagaa     1260

ugguuccacg acauacccuu acccuggcau gcgggagcag acacaggcac accgcacugg     1320

aacaauaaag aagcccucgu cgaguucaaa gacgcccacg caaaaaggca aaccgucgua     1380

guccugggcu cccaagaagg agccguacac accgcacucg ccggcgcccu agaagcagag     1440

auggacgggg ccaaaggccg acuaagcucc ggccaccuca aaugccgucu aaaaauggac     1500

aaacuacgcu uaaagggcgu gucauacuca cucugcacag cagccuucac auucaccaaa     1560

auaccggcag aaacacugca cggcacaguc acaguggaag ugcaauaugc cggcacagac     1620

ggcccaugua aagucccagc ccaaauggca guagacaugc aaacgcuaac accagugggc     1680

cgccuaauaa cagccaaccc cgucaucaca gaaaguacag aaaacucaaa aaugaugcua     1740

gagcuagauc ccccauuugg cgacucauac auaguaauag gcgucggcga aaagaaaauc     1800

acccaucacu ggcaccgauc aggaagcaca auuggaaaag ccuucgaagc gacaguaaga     1860

ggagccaaac gaauggcagu acugggagac acagccuggg acuucggaag cgugggcgga     1920

gcacugaacu cgcuaggaaa aggcauccac cagaucuucg gcgcagccuu caagucacuc     1980

uucggcggca ugagcugguu cucccagaua cucaucggca cgcuccucau guggcuagga     2040

cuaaacacga aaaacgguag caucucacug augugccucg cccucggagg aguccucaua     2100

uuccuaucga cagccguaag ugccgacgug ggaugcuccg ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5094
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 1 opt

<400>  5094
gggagaaagc uuaccaugcg caucauaaac gccagaaaag aaaagaaaag gcguggcgcc       60

gacacauccg uaggaauagu cggucuacua cucaccacag ccauggcagc cgaagucaca      120

aggagaggcu cagcauacua cauguaccuc gaccgcaacg augccggaga ggccauuuca      180

uucccaacaa cccuaggaau gaacaaaugc uacauccaaa uaauggaccu cggccauaca      240

ugcgacgcca caauguccua cgaaugcccu augcucgacg aaggaguaga accggacgac      300

gucgacugcu ggugcaacac caccucaacc ugggucgugu acggaacaug ccaccacaag      360

aaaggagaag ccagacgcag ccgccgggca gugacccucc ccucccacuc gauccgcaaa      420

cuccaaacac gcagccaaac cugguuagaa ucacgagaau acaccaaaca ucuaaucagg      480

gucgaaaacu ggaucuuccg aaacccaggc uucgcacucg ccgcagccgc aauagcgugg      540

uuacucggaa gcuccacaag ccaaaaaguc aucuaccucg uaaugauacu ucuaauagca      600

ccagccuaca gcauccgcug caucggaguc agcaaccgag acuuugucga gggcaugagc      660

ggcggcaccu ggguggacgu cguacucgaa cacggcggcu guguuacggu cauggcccag      720

gacaagccca cagucgauau agaacucguc acuacaaccg ucagcaacau ggcugaagua      780

cgaagcuacu gcuacgaggc cuccauaagc gacauggcaa gcgacucccg cugccccacc      840

caaggcgaag cauaccuuga uaaacaaucc gacacacaau auguaugcaa acgcacacuc      900

guagacagag gguggggaaa cgggugcgga cucuucggca aaggaucccu cguaaccugc      960

gccaaauucg ccugcuccaa aaagaugacc ggaaagagca uccaacccga aaaccucgaa     1020

uaucggauca ugcucuccgu ccacggaucc caacauagcg gaaugaucgu aaacgacaca     1080

gggcacgaaa ccgacgaaaa ccgcgcaaaa guagaaauca ccccgaacag cccccgcgcg     1140

gaagccaccc ucgggggcuu cggaagccug ggccucgacu gcgaaccccg aacaggacua     1200

gacuucuccg accucuacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccccu accauggcac gcaggcgccg acacgggcac accgcacugg     1320

aacaacaaag aggcauuagu cgaauuuaag gacgcgcacg caaaacgcca gacaguaguc     1380

gugcugggcu cacaagaagg cgccguacac accgcccuug ccggcgcacu agaagcagag     1440

auggacggcg caaaaggcag acuauccucc ggccaccuaa aaugcagacu aaagauggac     1500

aaacuccggc ugaaaggagu guccuacagc cuuugcaccg cugccuucac guucacaaaa     1560

auccccgccg agacccucca cggaacggua acaguagagg uccaguacgc aggcaccgac     1620

ggaccgugca aagucccagc ccaaauggcc gucgacaugc aaacgcucac ccccgucggc     1680

cgacugauaa ccgcgaaccc agucaucacc gaguccaccg aaaacuccaa aaugaugcua     1740

gaacuagacc caccauucgg ggacucauac aucgugauag gaguaggcga aaagaaaauc     1800

acccaccacu ggcaucguuc cggcuccaca aucggcaaag cauucgaagc cacagucaga     1860

ggcgccaaac gaauggccgu gcucggagac accgccuggg acuucggaag cguaggcggc     1920

gcccucaacu cccucggaaa aggaauccac caaaucuucg gggccgccuu caagucccuc     1980

uucggcggaa ugucaugguu cucucagaua cucaucggaa cucugcucau guggcuaggg     2040

cuuaacgcca agaauggcuc gaucucacuc augugccuag cauuaggagg aguccucaua     2100

uuccuaagca ccgcgguauc cgcagacgua gggugcagcg ucgacuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5095
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 1 opt

<400>  5095
gggagaaagc uuaccaugcg aauaauaaac gccagaaaag aaaagaaacg acgaggagcc       60

gacaccucag uaggcaucgu aggacuacua cucacgacag caauggcagc agagguaacc      120

cgccgcggga gugcauacua cauguaccuu gacagaaacg acgcgggaga agcgauaucc      180

uucccaacaa cgcucgggau gaacaaaugc uacauccaaa uaauggaccu cgggcauaug      240

ugcgacgcaa caaugucgua cgaaugccca augcuagacg aaggcguaga accagacgac      300

gucgacugcu ggugcaacac aaccagcacc ugggucguau acggcacgug ucaccacaag      360

aaaggagaag cccaacgcuc caggcgagcc gucacgcucc ccagccacag cacccgaaaa      420

cuccaaaccc gcagccagac auggcucgaa ucaagagaau acaccaaaca ccuaauacga      480

gucgaaaacu ggaucuuccg caaccccgga uucgcccucg cagccgcagc gauagcgugg      540

cuccugggca gcaguaccag ccagaaagua auauaccuug ucaugauacu ccucauagcc      600

cccgccuaca guauacgcug cauaggcgua agcaaccgcg acuucguaga aggaaugagc      660

ggcggaacau gggucgacgu ggucuuagaa cacggcggcu gugugacagu aauggcacaa      720

gacaagccca ccguagacau agaacugguc acaacaacag uaagcaacau ggcagaaguc      780

cgcucguacu gcuacgaggc uucaaucucc gacauggcau cagacucaag gugcccaacu      840

caaggagaag cauaccucga caaacaaucc gacacccaau acgucuguaa acgaacacuu      900

gucgaccgcg gaugggggaa cggaugcggc cucuucggga aaggaucccu cguaaccugc      960

gccaaauucg cgugcucgaa gaaaaugacg ggaaaaagca uacagcccga aaaccuugaa     1020

uacagaauaa ugcucagcgu ccacggauca caacacagcg gcaugauagu caacgacaca     1080

ggccacgaaa ccgaugaaaa ccgggccaaa guagaaauca cgccaaacuc cccacgagcc     1140

gaagcgacac ucggaggcuu cggaucccug gggcuagacu gcgaaccucg aacaggcuug     1200

gacuucagcg accuguacua ccuaacaaug aacaacaaac acuggcuggu acacaaagaa     1260

ugguuccacg acaucccacu ccccuggcac gccggagcag acaccggaac cccucacugg     1320

aacaacaaag aagcacucgu cgaguucaaa gacgcacacg ccaaaagaca aacaguagug     1380

guccucggga gccaagaagg agcaguacac acagcccucg caggcgcccu cgaagcagag     1440

auggacggag caaaaggacg ccugagcuca ggacaccuaa aaugcagacu aaaaauggac     1500

aaacuccgac uaaaaggcgu cucuuacuca cuauguacag cagcguuuac cuucacaaaa     1560

aucccagcgg aaacauuaca cggaaccguc accgucgaag uacaauaugc cggaaccgac     1620

ggcccaugca aaguccccgc acaaauggca gucgacaugc aaacacucac ccccgucgga     1680

cgccugauca cagcgaaccc aguaaucacc gagucaacgg agaacagcaa aaugaugcua     1740

gagcuggacc cgccauuugg agauuccuac auagucaucg gcgucggaga gaagaaaauc     1800

acacaccacu ggcaucgcag cggaagcaca auagguaaag cauucgaagc gacaguccgu     1860

ggagcaaaaa ggauggcggu ccucggcgau accgcauggg acuucggcag cguaggcgga     1920

gcccuaaaca gccucggaaa aggcauacac caaaucuucg gcgccgccuu caaaagccua     1980

uucggcggaa ugagcugguu cagccaaauc cuaauaggca cacuccucau guggcucggc     2040

cucaacacca aaaacggaag caucagccuc augugccuag cccucggcgg cguccuaauc     2100

uuccugucaa cagccgugag cgcagacguc ggcugcagcg uggacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5096
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 1 opt

<400>  5096
gggagaaagc uuaccaugcg cauuaucaac gcgcgaaaag agaaaaagag acgaggcgca       60

gacaccuccg uaggcaucgu cggccuacug cucaccaccg ccauggcagc agaagucacc      120

agaagagguu cagcguauua cauguaccuc gaccgaaacg acgcagggga agcaaucagc      180

uucccaacca cccuaggaau gaacaagugc uacauacaaa ucauggaccu gggacacaug      240

ugcgacgcca ccaugucaua cgaaugcccg augcucgacg aaggaguaga acccgaugac      300

gucgacugcu ggugcaacac caccagcacc uggguagucu acggaaccug ccaccacaag      360

aaaggcgaag cacgacgcuc gcgcagagca gucacccucc caucacacuc aacaagaaaa      420

cuacaggcca gaucacaaac auggcucgaa ucccgagagu auaccaaaca ccugauccgc      480

gucgaaaacu ggauauuccg aaaucccggc uucgcacugg ccgccgccgc cauagccugg      540

cugcucggca gcagcaccag ccaaaaagug aucuaccucg ucaugauacu gcucauagcc      600

ccagccuacu caauacgcug cauaggagua ucaaacagag acuucgucga ggggauguca      660

ggaggcaccu ggguagacgu cguccucgaa cacggcggau gcgugacagu cauggcccag      720

gacaaaccca caguagacau cgagcuaguc acaacaacag ucucaaacau ggccgaaguu      780

cgcucauacu guuacgaagc cagcaucagc gacauggcga gcgacucacg cugcccgacc      840

caaggagaag ccuaccugga caaacagucc gacacgcagu acgucugcaa gagaacccua      900

guagacaggg gcuggggcaa cggcugcggg cuauucggga aagguagccu uguaaccugc      960

gccaaauucg ccugcuccaa gaaaaugacc ggcaaaucga uccagcccga aaaucuagaa     1020

uaccgcauaa ugcucuccgu gcacggcagc caacacucag gcaugauugu aaacgacacu     1080

ggccacgaga cggacgaaaa ccgcgcaaaa gucgaaauca caccaaacuc cccacgcgcg     1140

gaggcaaccc ucggaggcuu cggaagccua ggccucgacu gugaaccccg caccggccua     1200

gacuucucag accuuuauua ccucacaaug aacaacaaac acuggcucgu ucacaaagaa     1260

ugguuucacg acaucccgcu ccccuggcac gcaggcgccg acacaggaac accccacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg caaaacgcca aaccgucguc     1380

guacucggcu cgcaagaagg agcaguacac accgccuugg caggcgcccu cgaagccgaa     1440

auggacggcg ccaagggccg gcucagcucc ggccaccuca aaugccgccu aaaaauggac     1500

aaacuacgac uaaaaggagu aagcuacagc cuaugcaccg cugcauucac uuucacaaaa     1560

auacccgcag aaacccuaca cggcaccguc acggucgaag uucaauacgc cggcaccgac     1620

ggacccugca aagucccagc ccaaauggca guagacaugc agacacucac cccagucgga     1680

agacuaauca ccgcaaaccc aguaauaaca gaauccaccg aaaacagcaa aaugaugcuc     1740

gaacucgacc cgccguucgg agacagcuac aucguaaucg gcguaggcga aaagaaaaua     1800

acacaccacu ggcaccgauc cggcuccacc aucggcaagg ccuucgaagc aaccguacga     1860

ggagccaaac gaauggcagu acucggagau acggcuuggg auuucggcag cgucggggga     1920

gcccuaaacu cacuaggaaa aggcauccau caaaucuucg gagcggcuuu uaaaagucua     1980

uucggaggca ugaguugguu cagccagaua cuaaucggca cacuacuaau guggcucggc     2040

cucaacacca aaaacggauc caucucacua augugucucg cccuaggcgg aguacuaaua     2100

uuccucagca ccgccgucag cgccgacgua ggaugcagug uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5097
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 1 opt

<400>  5097
gggagaaagc uuaccaugcg caucaucaac gcgcgcaagg agaagaaacg ccgaggcgca       60

gacacauccg ucggaaucgu agggcuacuc cucacaaccg caauggccgc agaagugaca      120

cgacgcggau cugcauacua uauguaccua gacagaagcg acgcaggaga agcaauuagc      180

uucccaacga cccucgggau gaacaaaugc uacauacaaa uaauggaccu cggacacaug      240

ugcgacgcaa cgaugucuua cgaaugcccc augcuagacg aaggggugga acccgacgac      300

gucgacugcu ggugcaacac cacaagcacg uggguagucu acggaaccug ccaccacaag      360

aaaggcgaag cccgcaggag caggagagcc guaacacucc ccucccacag cacccgaaaa      420

cugcaaacga gaucacaaac guggcucgaa uccagagaau auacaaaaca ccuaaucaga      480

gucgagaacu ggauauucag aaacccaggu uucgcccuag cagcagcagc aauagccugg      540

uuacuaggaa gcagcaccag ccaaaaagua auauaccuag uaaugauacu ccucauagcc      600

ccagcauaca gcauacggug uaucggagua agcaaccgag acuucgucga aggaaugucg      660

ggaggaacau ggguagacgu aguacucgaa cacggcggcu gcguaacagu cauggcccaa      720

gacaaaccaa caguagacau ugagcuagug acuacaacag ucagcaacau ggcggaaguc      780

cgcucguacu gcuacgaagc aucgaucagc gacauggcaa gcgacaguag augccccaca      840

cagggcgaag ccuacuuaga caaacaauca gacacccaau acguauguaa aaggacccua      900

gucgaucgcg gauggggcaa cggaugcggg cuauuuggca aagguucacu cguaaccugc      960

gcgaaauucg cgugcaguaa gaaaaugaca ggcaaaagua uacaaccaga aaaccucgag     1020

uaccgcauaa ugcuaucggu ccacggcucc caacacagcg gcaugaucgu caacgacacg     1080

ggacacgaaa ccgacgaaaa cagagcaaaa gucgagauca caccaaacuc accacgagca     1140

gaggcaaccc uuggcggauu cggcagucuc ggacucgacu gcgaaccgcg gacaggccuc     1200

gacuucucag accuauauua ccuaaccaug aacaacaaac acuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu accauggcac gccggcgccg acaccggcac accacacugg     1320

aacaacaaag aagcacucgu agaguucaaa gacgcgcacg cgaaacgaca aacaguagua     1380

guacuaggcu cacaagaagg agcaguccac acagcacugg ccggcgcacu agaagccgaa     1440

auggacggcg caaaaggacg ucuaaguagc ggucaccuaa aaugccgacu aaaaauggau     1500

aaacuacgcc uaaaaggggu cucauacucg cucugcaccg cagcauucac cuuuacgaaa     1560

aucccagccg aaacccugca cgggacagua accguagaag uccaauacgc cggcaccgac     1620

ggcccaugca aaguaccagc ccaaauggcc gucgacaugc aaacccugac cccgguggga     1680

agacugauca cagccaaccc agucaucaca gaaagcacgg agaacagcaa aaugaugcug     1740

gaacuugacc cacccuucgg cgacagcuac aucguaauag gggugggaga aaagaaaaua     1800

acacaucacu ggcaccgcuc agguagcaca aucggcaaag cauucgaggc aacagucaga     1860

ggagcaaaac ggauggcagu ccuaggagac acagccuggg auuucggaag cguaggaggg     1920

gcgcucaauu cacuaggcaa gggcauccac caaauauucg gagccgccuu caagucacug     1980

uucggcggaa ugagcugguu cagccaaauc cucaucggca cacuauuaau gugguuagga     2040

cugaacacua aaaacggauc caucagccuc augugccucg cgcugggcgg cguacugaua     2100

uuccugagca cagccguaag cgccgacguc gggugcucag uagacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5098
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 1 opt

<400>  5098
gggagaaagc uuaccaugcg cauaauaaac gccagaaaag aaaagaagag aaggggaaca       60

gacacauccg ugggcaucgu cggccuccug cucacaacag ccauggcagc cgaagucacc      120

cgaagaggca gcgcauacua cauguaccua gacaggucag acgcaggcga agcaauaucc      180

uucccaacaa cacuaggaau gaacaaaugc uacauccaga uaauggacuu aggacacaug      240

ugcgacgcaa cgaugucaua cgaaugcccc auguuagacg aaggaguuga accagacgac      300

guggacugcu ggugcaacac uacaucaacg uggguggugu acggaacaug ccaccacaag      360

aaaggagaag cgcgcagauc cagacgagca gugacccuac caagccauag cacccgaaaa      420

cuccaaacaa gaagccaaac cuggcuggag agcagggaau auaccaaaca ccucauaagg      480

gucgagaacu ggauauucag aaacccaggc uucgcacucg ccgcagcagc cauagccugg      540

cuccuaggcu caagcacaag ccaaaaagua auauaucucg ugaugauccu acuaaucgca      600

ccagcauacu caauacgcug caucggcguc aguaacaggg acuucguaga agggaugucg      660

ggaggcacau ggguggacgu uguacucgag cacggaggau gcgucaccgu cauggcacaa      720

gauaaaccca caguggacau cgaacucgua acuaccacag ugucuaacau ggcagaagua      780

cgaucauacu gcuacgaagc gucgauauca gacauggcca gugacucccg gugcccgaca      840

caaggagaag cauaccucga caaacagagc gacacacaau acguauguaa aagaacgcug      900

guagaccgcg gauggggcaa cggcugcgga cucuucggca aaggaucccu cguaaccugc      960

gcaaaauucg cgugcucaaa gaaaaugacc ggaaagagca uacaaccaga aaaccucgag     1020

uaccgcauaa ugcucucagu ccacggaagc cagcacagcg ggaugauagu caacgacaca     1080

ggccacgaaa cagacgaaaa ucgagcaaag guagaaauca caccgaacag cccccgcgcc     1140

gaagccacgc uaggaggauu cggcucccua ggacucgacu gugaaccgcg cacaggacua     1200

gacuucuccg accuauacua ccucacaaug aacaacaaac acuggcuagu gcacaaggaa     1260

ugguuccaug acauaccccu ccccuggcac accggagccg acaccggaac accacacugg     1320

aacaacaaag aagcccuggu agaguucaaa gacgcacacg caaaaagaca aacaguagug     1380

guccuaggca gucaagaagg cgcuguacac acagcacuag caggagcacu agaagccgaa     1440

auggacggcg caaaaggaag acugucguca ggacaccuca agugccgauu aaaaauggac     1500

aaacuaagac ugaaaggagu cucguacagc cucuguaccg cggccuucac auucaccaag     1560

auaccagccg aaacacugca cggaacagua acaguagaag ugcaguacgc cgggacggac     1620

ggaccaugca aagugccagc acaaauggcc guagacaugc aaacacucac gccagucggc     1680

cgccugauca ccgccaaccc cgucauaaca gaagggaccg agaacucgaa aaugaugcug     1740

gaguuggacc cacccuucgg agacagcuac aucguaaucg gagugggcga gaaaaagaua     1800

acgcaccacu ggcaccgcag uggaagcacc aucggcaaag ccuucgaagc caccguccgc     1860

ggcgcaaaga gaauggcagu ccuaggcgac acagcauggg acuucggaag cguaggaggc     1920

guucugaaca gccuaggcaa aggaauccac caaaucuucg gcgcagccuu caaaucacuc     1980

uucgggggca ugucaugguu cagccaaaua cuaauaggca cccuacucau guggcucggg     2040

cuaaacacua aaaacggaag caucagccua augugccucg cccugggagg cguacuaauc     2100

uuccuaagca cagcaguuuc agccgacgug ggaugcagcg ucgacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5099
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 1 opt

<400>  5099
gggagaaagc uuaccaugcg aauaauuaac gcuagaaaag aaaagaaaag acgaggagcc       60

gauacauccg uagggauugu cggacuacuc cuaacaacag cgauggcggc agaagugaca      120

cggcguggca gcgccuacua cauguaccug gaccgaaacg acgcaggcga agccaucuca      180

uucccaacaa cacuuggcau gaauaaaugc uacauccaaa uaauggaucu aggccacaug      240

ugcgacgcga caaugagcua cgaaugccca augcuagacg aaggcgucga acccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggaccug ccaccacaag      360

aaaggagaag cccgacgcuc aagacgcgcg guaacacuac caucacacuc aacccgaaaa      420

cugcaaaccc gcagucagac cuggcucgag ucgcgagaau acacaaaaca ccugauacga      480

guagaaaauu ggaucuuccg aaacccggga uucgcccucg ccgccgccgc aaucgccugg      540

cuccuaggau ccuccacaag ccaaaaaguc aucuaccucg uaaugauccu ccuaaucgca      600

ccagccuaca gcauaagaug cauaggagua agcaauagag acuucgucga aggaauguca      660

ggcggcacau ggguagacgu cguacuagag cacggaggau gcgucaccgc aauggcccaa      720

gacaaaccaa caguagacau cgaauuaguc acaacaacag uguccaacau ggcagaagua      780

cgcucauacu gcuacgaagc cagcaucagc gacauggcga gcgacucccg gugccccacg      840

caaggcgaag ccuaucucga caaacaguca gacacccaau acgucugcaa acgaacuuua      900

guugaccgcg gcuggggcaa cggaugcggc cucuucggca aaggaucacu cgucacgugc      960

gcgaaauucg ccugcagcaa gaaaaugaca gggaaaucaa uucagccuga aaaccuagaa     1020

uaccgcauca uguuaagcgu ccacggauca cagcacuccg gcaugcucgu gaaugauaca     1080

ggccacgaaa ccgacgaaaa uagagcgaaa gucgaaauca ccccgaacuc uccacgggca     1140

gaagcaacac uaggcggcuu cggcucccuc ggacucgauu gcgagccccg caccggacuc     1200

gacuuuagug accucuacua ccucacaaug aacaacaaac acuggcucgc acacaaagaa     1260

ugguuccacg acaucccgcu acccuggcac gccggagcag ccaccggcac accccauugg     1320

aauaacaaag aagcacucgu cgaguucaaa gacgcacacg cgaaacgcca aacagucgua     1380

gucuuaggaa gucaggaagg cgccguccac accgcucucg ccggcgcccu cgaagccgaa     1440

auggacggcg ccaaaggccg acucuccagc gggcaccuaa aaugccgacu caaaauggac     1500

aaacucagac ucaaaggcgu aucauacagc cucugcacag cagcauucac cuucacaaaa     1560

auccccgcug aaaccguuga cggcacagua accguagaag gccaauacgg gggcaccgac     1620

ggccccugca aaguccccgc gcaaauggcg guagacaugc aaacccucac gcccguaggc     1680

cgccucauaa cagccaaccc cgugaucaca gaaucaacgg aaaacagcaa aaugaugcua     1740

gaacuggauc ccccauucgg agacucauac auagugaucg gugucggaga aaagaaaauc     1800

acccaccacu ggcaccgcuc aggcucaaca auuggaaaag ccuucgaagc aacagucaga     1860

ggcgcaaaac gcauggccgu ccugggagac acagccuggg acuucggcuc agugggcggc     1920

gcccucaacu cccucgggaa aggcauccac cagaucaucg gagcggccuu caaaagccua     1980

uucggcggaa ugucaugguu cagccaaaua cuaauaggaa cacuacuaau guggcuagga     2040

cucaacacca aaaacggcag uaucucacuc augugccuag cccucggcgg agucuuaauc     2100

uuccucucaa cagcuguauc aggcggggua ggcugcuccg uagacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5100
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 1 opt

<400>  5100
gggagaaagc uuaccaugcg aaucauaaac gcacgcaaag aaaagaaacg acgagggacg       60

gacaccuccg ucggaaucgu aggccuccuu cugaccaccg caauggccgc cgaagucacc      120

cgccgaggcu cugcauacua cauguaccuc gaccgcagcg augccggaga agcgaucuca      180

uuccccacaa cacucggcau gaacaaaugc uacauucaaa ucauggaccu cggacacaug      240

ugcgacgcca caauguccua cgaaugcccc augcuagaug aaggcgucga accagacgau      300

gucgacugcu ggugcaauac aaccagcacc ugggucgugu acggcacaug ccaccacaag      360

aaaggggaag ccagacgcag ccgacgagca gucacacuac cgucacacag cacacguaaa      420

cuccaaaccc gcagccaaac auggcuggag uccagagagu acaccaaaca ccugauaagg      480

guagaaaacu ggauauuccg caacccuggc uucgcccucg ccgccgcagc aaucgccugg      540

cuacucggcu caucgaccuc ccaaaaagua aucuaccucg ucaugauacu ucucaucgcc      600

cccgcauacu ccauccgaug cauaggagug uccaaccgcg acuucgugga aggcaugucc      660

ggcggaaccu gggucgacgu aguacuagaa cacggaggau gcguaacggu cauggcacaa      720

gacaaaccca caguugauau agagcucgua accaccaccg ucagcaacau ggccgaagua      780

cgcucauacu gcuacgaagc cuccaucucg gacauggcua gcgauucccg cugccccaca      840

caaggagaag cguaccugga caaacaaucg gacacacaau acguaugcaa acggacccua      900

gucgaccgcg guuggggcaa cggcugcgga cuuuucggaa aaggaagccu cguaacaugc      960

gccaaauucg caugcucgaa aaagaugacc ggcaaaagca uacaaccaga gaaccucgag     1020

uacagaauaa ugcucucagu ccacggcucc cagcacuccg gcaugauugu gaaugacacg     1080

ggccacgaaa ccgacgagaa ucgcgcuaag gucgaaauca cacccaauuc accacgcgcg     1140

gaggccacac ucggcggguu uggaagccuc ggccucgacu gcgaacccag aacaggacua     1200

gacuucagcg accuguacua ccucaccaug aacaauaaac acuggcuagu ccacaaagaa     1260

ugguuccacg acauccccuu accauggcau gcaggcgccg acaccggcac accccacugg     1320

aacaacaaag aagcccucgu agaguucaaa gacgcacacg ccaaacgaca aacaguagug     1380

guacugggau cucaggaagg agccguacac acagcccuag caggcgcauu agaagccgaa     1440

auggacggag ccaaaggucg ccucucuagc ggacaucuca agugccgccu gaaaauggac     1500

aaacucagac uaaagggagu cucuuacuca uuaugcaccg cagccuucac auuuuccaag     1560

aucccagccg aaacacugca cggcaccguc accgaggaag uacaguacgc cggaaccgac     1620

ggcccaugca agguaccagc ccaaauggcg gucgacaugc aaacacuaac ccccgucggc     1680

cgacucauua cagcuaaccc cguuaucaca gaaucaaccg aaaacucgaa aaugaugcua     1740

gaacuagacc cacccuucgg agacuccuac aucgucaucg gcguaggcga aaagaaaauu     1800

acacaccacu ggcaccgcag uggcagcacg auaggaaaag cauucgaagc aacuguccgc     1860

ggagccaaac gcauggcagu ccucggggac accgcauggg acuucggaag cgucggaggc     1920

gcccucaacu cgcucggaaa aggaauccac caaauuuucg gagcagcguu caagucccug     1980

uucgggggca ugagcugguu cagccaaaua cucauaggaa cacuacucau gugguuaggc     2040

uuaaacacua agaauggcag caucagccuc augugccucg cccuaggcgg aguacucauc     2100

uuccucagca cagccgugag cgccgacgua ggcugcagcg ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5101
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 1 opt

<400>  5101
gggagaaagc uuaccaugcg cauaaucaac gcccggaaag aaaagaaacg acgaggcacu       60

gacaccuccg ucggaauagu cggacuacua cuaacuacag ccauggccgc agaagucaca      120

aggcgcggau cagcauacua cauguaccua gaccgcucag acgccggcga agccaucagc      180

uucccaacca cacuaggaau gaacaaaugc uacauacaga uaauggaccu gggacacaug      240

ugcgacgcca caaugagcua cgaaugccca augcuagacg aaggcguuga acccgacgac      300

guagacugcu gguguaacac gaccagcacc ugggucguau acggaacaug ccaccacaag      360

aaaggcgaag cuaggcgcuc ccgacgcgcc gucacccuac cgagccauuc caccagaaaa      420

cuccaaacga ggucccagac cuggcuggaa ucccgagaau acacaaaaca ccuuauacga      480

guagaaaacu ggauauuccg aaacccgggg uucgcccugg ccgccgcugc cauagccugg      540

cuccucggcu ccuccacaag ccaaaaagua auauaccugg uaaugauccu acuaauagcc      600

cccgccuaca gcaucaggug caucggcguc ucaaacagag acuucgucga aggcaugucc      660

ggcggaaccu ggguggacgu aguccuggag cauggcggau gcgugacagu aauggcccaa      720

gacaagccaa ccguagacau cgaacuggua acuacaaccg ucagcaacau ggccgaaguc      780

cgaagcuacu gcuacgaagc cagcauaagc gacauggccu ccgacucccg gugcccaaca      840

caaggcgaag ccuaccuaga caaacaaucc gacacgcaau acguaugcaa acgaacccua      900

gucgacagag gauggggcaa cggcugcggc cucuucggca aaggaucacu cgucacgugc      960

gccaaguucg ccugcaguaa gaaaaugaca ggcaaaucga uacaacccga aaaccuagaa     1020

uaccgcauca ugcuaagcgu acacggcagc caacacucag gaaugaucgu gaacgacaca     1080

ggccacgaaa cagacgaaaa ucgagcaaaa guagaaauca ccccuaacag cccacgugcc     1140

gaagcaacac uaggaggauu cggcagccua ggccuugacu gugagccacg aaccggacuc     1200

gacuucuccg accuguacua ccucacgaug aauaacaaac auugguuagu ccacaaagaa     1260

ugguuccacg acauaccauu gccauggcac gcaggcgcgg auaccggaac gccacacugg     1320

aacaacaaag aagcacucgu cgaguucaaa gaugcacacg caaaaagaca gaccguaguc     1380

guacuuggca gucaagaagg cgccguccac accgcgcuug ccggagcccu agaagccgaa     1440

auggacggcg ccaaaggccg acucuccucc ggacaccuca aaugccgacu gaaaauggac     1500

aaacuccgac ucaaaggcgu aagcuacagc uugugcaccg ccgccuucac auucacaaaa     1560

auaccagcag aaacucucca cggcaccgua accguagaag uacaguacgc cgggacagac     1620

ggccccugca agguaccagc acaaauggcc gucgacaugc aaacccuaac acccguuggc     1680

agacuaauca cagcgaaccc aguaaucacc gaaagcaccg aaaauagcaa gaugaugcuc     1740

gaacucgauc ccccauucgg cgacagcuac auagucauag gagucggcga gaagaaaauc     1800

acccaccacu ggcaccgaag cggcagcacc auaggaaaag ccuucgaagc cacagugaga     1860

ggcgcgaaaa gaauggcagu ccucggcgac acagcguggg acuucggcag cgucggcggc     1920

gcacucaacu cacucggcaa aggcauccac caaaucuucg gcgcagccuu caaaagccuc     1980

uucggcggca uguccugguu cagccaaaua cuaaucggga cccuacugau guggcuagga     2040

cuaaacacca aaaacggauc gaucucccuc augugcuuag cacucggcgg cguccuaaua     2100

uucuuaagca ccgccguauc cgcagacgug ggcugcagug ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5102
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 1 opt

<400>  5102
gggagaaagc uuaccaugcg aauaaucaac gcacgcaaag agaaaaagag aagaggaaca       60

gacacaucag ucggcaucgu cggauuacug cucacgacgg caauggcggu agaggugacc      120

cgcaggggaa acgccuacua cauguaccua gacagaagcg acgcaggaga ggcuaucucc      180

uucccgacca caaugggcau gaacaaaugc uacauccaaa ucauggaccu agggcauaug      240

ugcgacgcaa ccaugagcua cgaaugccca auguuagacg agggagucga accagacgac      300

guugacuguu ggugcaauac cacaagcacc ugggucguau acggcacaug ccaccacaag      360

aaaggggaag cgaggcgcag ucgacgcgcc gucacccuac ccagccauuc cacacgcaag      420

uuacaaacgc gcagccaaac guggcuagaa agccgagaau acaccaaaca cuuaaucaga      480

gucgaaaacu ggaucuucag aaacccggga uucgcacuag cagccgccgc gaucgccugg      540

cuacuagguu cgagcacaag ccaaaaaguc aucuaccuag ugaugauacu ccuaauagcc      600

cccgcauaca gcauacggug uauaggcgua ucaaacagag auuucgucga gggaaugucc      660

ggcggaacau ggguagacgu aguacuggaa cacggcggau gcguaacagu cauggcacag      720

gacaaaccca cagucgacau agaacucguc accaccaccg ucagcaacau ggccgaagua      780

cgcagcuacu gcuacgaggc aucaauaucc gacauggcau ccgacagcag augcccaacg      840

cagggagagg ccuaccuaga caaacaaucc gacacacagu acgugugcaa aagaacccua      900

guagaccgag guuggggcaa cggcugcgga cuauucggga aaggcucgcu aguaaccugc      960

gccaaauucg ccugcagcaa gaaaaugacc ggcaaaucaa uucaaccaga aaaccucgaa     1020

uaccgaauca ugcucagcgu ccacggaagc caacacagcg gaaugauagu aaacgacacc     1080

ggccacgaaa cagacgagaa cagagcgaaa gucgaaauaa cacccaacag uccccgcgca     1140

gaagccaccc ugggaggauu cggcagccuc ggccuagacu gcgaaccacg aacaggacua     1200

gacuucagcg accuguacua ccugacaaug aacaacaaac auuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu cccuuggcac gcuggcgccg acacaggaac accccacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gaugcacacg caaaacgcca aaccguagug     1380

guccugggcu cacaggaagg agccgugcac acagccuuag cgggcgcgcu cgaagccgaa     1440

auggacggag ccaaaggcag auuaagcagc ggacaccuca aaugccggcu gaaaauggac     1500

aaacugcgac ucaaaggagu auccuacagc cucuguacag cggccuuuac auucacaaaa     1560

auaccagcgg aaacgcuuca cgggacagua acaguugaag uacaauacgc uggaacagac     1620

ggacccugca aaguaccggc acagauggcc gucgacaugc aaacccuaac cccaguagga     1680

cgccugauaa cggccaaccc agugaucacc gaaagcaccg aaaacucaaa aaugaugcuc     1740

gaacucgacc cgccauucgg cgacagcuac auagucauag gagugggaga aaagaaaaua     1800

acgcaccacu ggcaccgcag cggcaguaca aucggaaaag ccuucgaagc aaccgugcgc     1860

ggcgcaaaaa gaauggccgu acuaggagac accgcuuggg acuucggcag cguaggcgga     1920

gcccucaaca gccucgggaa ggggauccac caaaucuucg gagcagccuu caagagccuu     1980

uucggaggca uguccugguu cagccagaua cuaauaggga cccuacuggu auggcuaggu     2040

cucaacacca aaaacggcag cauaagucuu augugccucg cacuaggcgg aguacuaaua     2100

uuccuaagca cagcagugag ugccgacgua ggcugcagcg uagacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5103
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 1 opt

<400>  5103
gggagaaagc uuaccaugcg aaucauaaac gcccgcaaag aaaagaaaag gagaggcaca       60

gacacaagcg uaggaaucgu ggggcuccug cuaacaacag ccauggcggc cgaggucacc      120

cguagaggca gcgcguacua cauguaccua gaccgcagcg acgccggaga ggccaucagc      180

uucccgacaa cccuaggcau gaacaaaugc uacauacaaa uaauggaccu cggccacaug      240

ugcgacgcga ccaugucaua cgaaugccca augcuggacg aaggagucga acccgacgac      300

guagacugcu ggugcaacac aacgagcacg uggguagucu acggaaccug ccaccacaag      360

aaaggcgaag cacgacgauc ccgacgagcg gucacgcucc caagccacuc cacccgaaaa      420

cuccagacca gaagccaaac cuggcuagaa uccagggaau acaccaaaca ccucauccgc      480

guagaaaacu ggauauuccg aaaccccggc uucgcacucg cagcagcagc cauagcaugg      540

cuacucggca gcagcacaag ccaaaaagua auauaccugg ugaugauacu acugaucgcc      600

cccgccuaca gcauccgcug cauuggaguc aguaacagag acuucguaga gggcaugagu      660

ggaggaacau ggguggacgu cguccuggaa cacggaggau gcgucacagu aauggcacaa      720

gacaaaccca cagucgacau cgaacucguu acaacgaccg ucucaaacau ggccgaagua      780

cgcagcuacu gcuacgaggc aaguauaagc gacauggcaa gcgacagccg augcccgacc      840

caaggagaag cauaccugga uaaacaaagc gacacgcaau acgucugcaa aagaacccua      900

gucgaccgcg gaugggggaa cggaugcgga cuauucggaa aagggagccu cguaacaugc      960

gcaaaauucg cgugcucaaa gaaaaugaca ggcaaaagca uccaaccaga aaaccuagaa     1020

uaccgaauca ugcuaagcgu acacggaucc caacacagcg gaaugauagu caaugacacg     1080

ggacaugaaa cagacgaaaa cagagccaaa gucgaaauaa caccaaacag uccccgcgcg     1140

gaagccaccc ucgggggauu cggaagccuc ggcuuagacu gcgagccgcg cacaggacua     1200

gacuucagcg accuauacua cuuaaccaug aauaacaaac auuggcucgu ccacaaagag     1260

ugguuccacg acaucccacu uccguggcac gccggcgcgg acacaggcac accgcacugg     1320

aacaacaaag aagcccucgu ggaguucaaa gacgcccacg ccaaacgaca aacagucgua     1380

guucuagggu cccaagaagg cgcaguacac accgcacucg caggcgcccu cgaggccgaa     1440

auggacggcg ccaaaggccg acuguccagc ggacaccuca agugcagacu aaaaauggac     1500

aagcuccgcc uaaaaggcgu aucauacucg cuauguacag cggcauucac auucaccaaa     1560

aucccagccg aaacccucca cggaaccgua acaguagaag uacaauacgc aggaacagac     1620

ggcccgugca aaguaccggc ccaaauggca guagacaugc aaacacucac acccgucgga     1680

aggcuaauca cagcaaaccc agucauaacg gaaagcacag aaaacagcaa aaugaugcuc     1740

gaacuagacc caccguucgg cgacagcuac auaguuauag ggguaggaga aaagaaaaua     1800

acacaccauu ggcauagauc cggcagcaca aucgggaaag cauucgaagc gacagucaga     1860

ggagcuaaac gaauggccgu acucggcgac acagccuggg acuucggauc cgugggcggg     1920

gcccugaaca gucucggcaa aggaauccac caaaucuucg gagccgcauu caaaucgcua     1980

uucggcggca uguccugguu cucccagauc cuaaucggaa cccuauuaau guggcucggg     2040

cuaaacacca aaaacggauc cauuucccug guaugccucg cccucggagg aguacucaua     2100

uuccugagca cagccgucag cgccgaugua ggaugcuccg uggacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5104
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 1 opt

<400>  5104
gggagaaagc uuaccaugcg cauaaucaac gcaagaaaag aaaagaaacg ccgaggcaca       60

gacaccagcg ugggcauagu cggacuacuc cuaacaaccg ccauggccgu ugaagucaca      120

cgacgaggaa gcgcauacua cauguaccua gaccguagcg acgccggaga agcaauaucc      180

uucccaacca cccuaggaau gaacaaaugc uacauccaaa uaauggaccu aggccauaug      240

ugcgacgcua cgaugaguua cgaaugcccc augcuggacg aaggcguaga gccggacgac      300

gucgacugcu ggugcaacac caccagcacc uggguagucu acggaaccug ccaccacaag      360

aaaggcgagg cacgcagaag ccgccgagca gucacccuac caucccacuc aacacgcaaa      420

cuacaaacaa gaucccaaac cuggcuggaa agccgcgaau acacaaaaca ccugauacga      480

gucgagaacu ggaucuuccg aaacccaggc uucgcccucg ccgcagccgc caucgcgugg      540

cuacucggca gcuccacuag ccaaaaagug auauacuuag uaaugauacu ccucaucgcc      600

ccagccuacu cgauccgcug caucggcguc agcaauaggg auuuuguaga aggcaugagc      660

ggcgguacuu gggucgacgu cgugcuagag cacggcggcu gcgucacagu aauggcacaa      720

gacaaacccg ccgucgacau cgaacucgua acgacgacag uaucaaacau ggccgaaguc      780

agaucguauu gcuacgaagc cuccaucagc gacauggcca gcgacucccg cugcccaaca      840

cagggagaag cauacuuaga caaacaaagc gacacacaau acguaugcaa acgaacccua      900

gucgaccgcg gaugggggaa cgggugcggu cuauucggaa aaggaucacu cguaaccugc      960

gccaaauucg cgugcagcaa gaaaaugacc ggcaaaucga uacaaccaga aaaccucgaa     1020

uacagaauca ugcuauccgu ccacggcucc caacacucug gcaugaucgu aaacgauacc     1080

ggccacgaaa ccgacgaaaa ccgagccaaa guggaaauca ccccgaacag cccucgggca     1140

gaagccacgc ugggcggguu cgggagccua ggacucgacu gugaaccacg caccggacua     1200

gauuucagcg accuauacua ccugaccaug aacaacaaac acugguuagu ccacaaagaa     1260

ugguuccacg acauaccacu cccauggcac gcaggcgccg acacaggcac cccacacugg     1320

aacaacaaag aagcacuggu agaguucaaa gacgcccacg ccaaacgcca aacggucgua     1380

gugcuagggu cacaggaagg cgcgguccac accgcccucg ccggcgcccu ggaagcagaa     1440

auggacggug ccaaaggacg ccucagcuca ggacaccuaa aaugccgacu caaaauggac     1500

aaacugcgcc ucaaaggggu cucauacuca cuaugcaccg ccgccuucac cuucaccaaa     1560

aucccagccg aaacacucca cggaacagua accguggaag uccaguacgc cggcaccgac     1620

ggacccugca aggugccagc acaaauggcc guagacaugc aaacccucac cccagucgga     1680

cgccuaauaa cugccaaccc cgucaucacc gaaagcacag aaaacucaaa aaugaugcuu     1740

gaacuggauc ccccauuugg cgacagcuac aucgucaucg ggguuggcga aaagaaaauc     1800

acacaccacu ggcaucgcuc aggcuccacc aucggcaaag cguucgaagc caccguccgc     1860

ggcgcaaaac gcauggccgu acugggagac accgcauggg acuuuggaag ugucggaggc     1920

gcccugaacu cacuagggaa aggaauacac caaaucuucg gagcagcguu caaaagccuc     1980

uucggcggaa ugaguugguu cagccaaauc cuuauaggaa cccuccucgu auggcucggg     2040

uuaaacacca aaaacgggag cauuagccuu acaugccuag cccucggcgg cguacucaua     2100

uuccucagca ccgcugucuc ggccgacguc ggcuguuccg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5105
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 1 opt

<400>  5105
gggagaaagc uuaccaugcg gaucaucaac gccagaaaag aaaagaaacg gcgcggagcg       60

gacacaucag ucgggaucgu aggccuacuu cucaccacgg caauggcagu cgaaguaaca      120

agacgcggaa gcgccuacua uauguaccuc gaccgcuccg acgccggaga agcaaucagc      180

uuccccacua cccuaggcau gaacaaaugc uacauccaaa ucauggaccu cggacacaug      240

ugugacgcca cgaugucaua cgaaugcccc augcucgacg aaggagugga acccgacgac      300

guagacugcu ggugcaacac cacaucaaca uggguugucu acggaaccug ccaccacaag      360

aaaggagaag cccgucgaag ccggcgagcc guaacacucc cuucgcacuc aacccgaaaa      420

cugcaaacaa gaucccaaac auggcuagaa agucgugaau acacaaaaca ucuaauacgu      480

guggagaacu ggaucuuccg aaaccccgga uucgcauuag cagccgccgu cauagccugg      540

cugcuaggaa gcagcaccag ccaaaaagua auauaccugg ucaugauccu acucauagcg      600

ccagccuacu ccaucagaug uauaggcguc ucaaaccggg acuucgucga gggaaugucc      660

ggaggcacuu gggucgacgu gguacuggaa cacggcggau gcgucacagu cauggcacaa      720

gacaaaccaa cuguagacau agaacucguc acgaccacgg ucagcaacau ggccgagguc      780

agaagcuacu gcuacgaagc cagcauuuca gacauggcaa gcgacagccg gugcccgacc      840

caaggagaag cguaccuaga caagcaaagc gacacccaau acgucugcaa aagaacccuc      900

gucgaccggg gcuggggcaa cggcugcgga cucuucggca aaggaagucu aguaacuugc      960

gcaaaauuug cgugcucgaa gaaaaugacc ggcaaaucaa uccaacccga gaaccuagag     1020

uacagaauaa ugcuaucagu ucacggcagu cagcacuccg gaaugaucgu aaacgacacc     1080

ggccacgaaa ccgacgaaaa ccgcgcaaaa gucgaaauaa cacccaacag cccccgagcc     1140

gaagccacac ucggaggguu cggcucccua ggacuagacu gcgagccacg caccggacuc     1200

gacuucucag accuauacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccgcu ccccuggcac gccggagcgg acaccggcac accccacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacaug caaaacgcca aaccguaguc     1380

guacucggca gccaggaagg ggccgugcac acagcccuag caggagcccu cgaagccgaa     1440

auggacggag ccaaaggacg acucucaagc ggccaccuca aaugccgacu aaaaauggac     1500

aaacuacgac uaaaaggcgu cagcuauagc cugugcaccg cagccuuuac cuuuacuaag     1560

auucccgcgg aaacccuaca uggcaccguc accguagagg uacaauacgc cggcaccgac     1620

ggcccaugca aaguccccgc ccaaauggcc gucgacaugc aaacgcucac accagucgga     1680

agacuaauaa cagccaaccc cgucaucacc gaaaguaccg aaaacaguaa aaugaugcua     1740

gaacucgauc cacccuucgg agacucauac aucgucaucg gagucggcga aaagaaaaua     1800

acacaccauu ggcauagguc gggcuccaca auaggaaaag cauucgaagc caccguccgc     1860

ggugcaaaac gaauggcagu ccuaggggac acagcauggg acuucggcag cgucggaggu     1920

gcacuaaacu cccucggcaa aggcauccac caaaucuucg gcgcagccuu caaaucccug     1980

uucggaggca uguccugguu cagccaaauc cucaucggca cccuauuagu auggcuaggc     2040

cucaacacga aaaauggcuc gauaucacua acaugucucg cccuaggcgg aguccucauc     2100

uuccuaagca ccgccguauc cgcagacguc ggaugcuccg uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5106
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 1 opt

<400>  5106
gggagaaagc uuaccaugcg aauuauaaac gcccgcaaag agaagaaaag acgcggagcc       60

gacacauccg uggguaucgu cggacuccuc cuaaccaccg ccauggccgu cgaaguaacc      120

cgacgcggau ccgcguacua cauguaccuu gaccgcucag acgcggggga agccauaucu      180

uuucccacca cacuaggagu caacaaaugc uacauccaaa ucauggaccu aggccacaug      240

ugcgacgcca caaugagcua cgagugcccc augcucgacg aagggguuga accagacgac      300

guugacugcu ggugcaacac cacguccacc uggguugucu auggaaccug ccaccacaag      360

aaaggcgaag cccgccgguc ccgccgagcc gucacccugc ccagccacag cacacgcaaa      420

cuacaaaccc gaucucaaac auggcuagag ucacgagaau auacaaaaca ccucauccga      480

gucgaaaauu ggaucuuccg caacccaggc uucgcgcucg ccgcagccgc caucgcaugg      540

cuccucggau ccucaaccuc acaaaaaguu auauaccugg uaaugauauu acucauagca      600

ccagccuacu caauacgaug cauaggaguc uccaaccgcg acuucgucga gggcaugagc      660

ggaggcaccu ggguagacgu cguccuagaa cacggaggcu guguuaccgu cauggcgcaa      720

gacaaaccca cagucgacau cgaacucgua acaaccaccg ucuccaauau ggcggaggua      780

cgaucauauu guuacgaagc aucaauauca gacauggcga gcgacagucg cugcccgacc      840

caaggcgaag ccuaucuuga caaacaaagc gacacacagu acguaugcaa acgaacacuc      900

guagaccgag gcuggggcaa cggcuguggc cuguuuggca aagguagccu gguaacaugc      960

gcaaaauucg ccugcuccaa gaaaaugacc ggcaaaucaa uacaacccga aaaccuagaa     1020

uaccgcauca ugcuguccgu ccacggcagc caacacagcg gcaugaucgu caacgacacc     1080

ggacacgaaa ccgacgaaaa ccgcgccaaa guagagauaa caccuaacuc accccgagcc     1140

gaagccacac ucggaggcuu uggcagccua ggacucgacu gcgaaccccg aacaggacuc     1200

gacuucuccg accuauauua ccuaacaaug aacaacaaac acuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu acccuggcac gccggagccg acaccggaac accccacugg     1320

aacaacaaag aagcacucgu cgaguucaag gacgcacacg ccaaacggca gaccgucguu     1380

guccuaggcu cacaagaagg cgcgguccac accgcucucg caggcgcccu cgaagcagaa     1440

auggacggcg ccaaggggag gcucuccucc ggacaccuca agugccgccu aaaaauggac     1500

aaacuacggc ucaaaggagu aagcuacagc cucugcacag cagcauucac auucaccaaa     1560

auaccagcag aaacgcuaca uggaacaguc acggucgaag uacaauacgc gggcacagac     1620

ggacccugca aagugcccgc acaaauggcg guugacaugc aaacccuuac gccagucggc     1680

cgacucauca ccgccaaccc cgucauuaca gaauccacag aaaacuccaa aaugaugcuu     1740

gaacuggacc cacccuucgg cgacucguac auaguaauag gcgucggcga caagaaaauc     1800

acccaccacu gguaccgcuc cggcucaaca aucggaaaag cauucgaagc aaccguccga     1860

ggugccaaac guauggcagu acuaggcgac accgccuggg acuucggcuc cguaggagga     1920

gcacucaacu cccucggaaa aggaauccau caaauauucg gagccgccuu caagucccua     1980

uucggcggca ugucaugguu uagccagauc cucauaggaa cccugcuagu auggcuaggc     2040

cucaacacca aaaacggguc aaucucuuua accugccuag cauuaggggg aguucucaua     2100

uuccucagca cagcaguaag cgccgacgua ggcuguuccg ucgauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5107
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 1 opt

<400>  5107
gggagaaagc uuaccaugcg cauaaucaac gcacgcaaag aaaagaaaag acgcggagca       60

gacacaagcg ugggaauugu aggccugcua uuaaccacug ccauggcggu agaaguaaca      120

cgacgcggau cagcauacua cauguaccug gacagaagcg acgcuggcaa agccaucucc      180

uucccaacaa cgcugggagu aaacaaaugc uacauccaaa uaauggaccu agggcacaug      240

ugcgacgcca caaugagcua cgaaugcccg augcucgacg agggaguaga accagacgac      300

gucgacugcu ggugcaacac aacuagcaca uggguaguau acggcaccug ucaccacaag      360

aaaggagaag cacgccggag ccgacgggca gucacgcuac ccucacacag cacacgaaaa      420

cuacaaacuc gcucccaaac cuggcucgaa agccgagaau acaccaaaca ccuaauccgc      480

gucgagaacu ggaucuuccg caacccagga uucgcacuag cagcugcagc aaucgcgugg      540

cugcucggaa gcagcacaag ccaaaaagua auauaccuag uaaugauacu ccucaucgcc      600

cccgccuaua guauacggug caucggcguc agcaaccgag acuucgucga aggcaugagc      660

ggcggcacgu gggucgacgu cguccucgaa cauggcggau gcgucacagu aauggcccag      720

gacaaaccaa ccgucgacau cgaacuuguc acaacgaccg ugagcaacau ggccgaaguc      780

cgcuccuacu gcuaugaagc cuccaucuca gauauggccu cagacagccg augcccaacc      840

cagggcgagg cauacuugga caaacaaagc gacacccaau acgucugcaa gcgaacacug      900

gucgaccgag ggugggguaa uggaugcggc cuauucggca agggaagccu agucaccugu      960

gcaaaguucg ccugcucaaa gaaaaugaca gggaaaucca uccaacccga aaaccucgag     1020

uaccgcauaa uguugagcgu ccacggcucc caacacagcg gcaugauagu aaacgacauc     1080

ggccaugaaa ccgacgaaaa ucgagcaaaa guagaaauaa caccuaacag uccgagagcg     1140

gaagcaacac uaggaggcuu cggcucgcua ggccuagacu gcgaaccccg aaccggacuc     1200

gacuucagcg accuauacua ucucacuaug aacaacaaac acuggcucgu gcacaaagag     1260

ugguuccacg acaucccgcu ccccuggcac gcaggagccg acacaggcac cccacacugg     1320

aacaacaaag aagcgcuagu cgaguucaaa gacgcacacg cuaaaagaca aaccgugguc     1380

guccuaggcu cacaagaagg cgcaguacac acagcacuag ccggcgcucu cgaagcagaa     1440

auggacggag ccaaaggucg acuauccucc ggccaccuaa aaugccgccu caaaauggau     1500

aaacuccgcc ucaaaggcgu aucauacagu cuaugcaccg ccgcauucac auucacaaaa     1560

auaccagccg aaacccucca cggcaccgua accguagaag uacaguacgc aggaaccgac     1620

ggaccaugca aagugccagc ccaaauggca gucgacaugc aaacacucac uccagucgga     1680

cgacuaauca ccgcuaaccc cgucauaacc gagagcacgg aaaauuccaa gaugaugcua     1740

gaacuggauc ccccguucgg cgacagcuac auagucaucg gagucggaga caagaaaauc     1800

acacaccacu ggcaccgaag cggcagcacc aucggaaaag cguucgaagc aaccgucaga     1860

ggcgccaaac gaauggccgu acucggagac acggccuggg acuucggcuc cgucggcgga     1920

gcacucaauu cccucggaaa agguauccac caaauuuucg gcgccgcauu caaaucacua     1980

uuugggggca uguccugguu cucgcagaua cuuaucggca cccuccuagu auggcuagga     2040

cuaaacacca aaaacggcag cauaagccuc acaugccuag cccucggagg gguccuaaua     2100

uuccugagca cagccguaag cgcugacguc ggcugcagcg ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5108
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 1 opt

<400>  5108
gggagaaagc uuaccaugag aaucaucaac gcaagaaaag aaagaaaaag aagaggcgcc       60

gacacaucaa ucggcaucgu cggccugcua cuaacaacag cuauggcagc agaaaucacc      120

cgacgaggcu cagcguacua cauguaccuc gacagaagcg acgccggaaa agcuaucagc      180

uucgccacga cacugggagu caacaagugc cacgugcaga uaauggaucu cggacacaug      240

ugcgacgcaa ccaugaguua ugagugcccc augcuagacg aaggcguaga gccagacgac      300

guagacugcu ggugcaacac aacaagcacc uggguaguau acggcacaug ccaccacaag      360

aaaggagaag caagacgaag caggcgcgca guaacacuac ccucacacag cacacgaaaa      420

cuccaaacca gaagccagac auggcucgag agccgugaau acacaaaaca ccuaauaaaa      480

guggaaaacu ggauauuccg caacccaggg uucacccuag uagccguagc aaucgcgugg      540

cuucucggga gcagcaccuc ccagaaaguc auauaccucg uaaugauccu ucucauagcu      600

ccggccuaca gcauacgaug cauaggcgua agcaaccgcg acuucgucga aggaauguca      660

gggggaacau ggguggacgu aguacuagaa cacggaggcu gcgucacggu aauggcacaa      720

gacaaaccaa ccgucgauau cgagcuaguc accaccacag ucagcaacau ggcggaagua      780

cgaagcuacu gcuacgaagc cucaaucagc gacauggcga gcgacucucg cugcccaaca      840

caaggcgaag cauaccucga uaaacaaagc gacacgcagu acgucugcaa acgcacccuc      900

gucgacagag gguggggcaa cggguguggc cuauuuggaa agggaucauu agucacaugc      960

gcaaaauuca caugcagcaa gaaaaugaca gggaaaagca uccaaccuga gaaccucgaa     1020

uaccgcauaa ugcucagcgu ccauggauca caacacuccg gcaugaucgu caacgacacg     1080

ggacacgaaa cagaugaaaa ucgagcgaaa guagagguca caccgaauag ccccagagcc     1140

gaagccacac uaggaggauu cggaagccuc ggccuggacu gcgaaccgcg aaccggccuc     1200

gacuucagcg accuauacua ccucaccaug aacaacaaac auuggcuagu ccacaaagaa     1260

ugguuccacg acaucccccu ccccuggcac gcaggcgccg acacgggaac cccucacugg     1320

aacaacaaag aagcacuggu ugaguucaaa gacgcacacg ccaaaagaca aacaguagua     1380

guauuaggga gccaggaagg agccguacac acagcccucg caggcgcccu agaagccgaa     1440

auggacggag ccaaaggcag acuguucagu gggcaccuca aaugcaggcu aaaaauggac     1500

aaacuacgcc uaaaaggcgu aagcuacagc cuaugcacag ccgcauucac cuucacaaag     1560

guccccgcgg aaacacugca ugggacggua acaguagaag ugcaauacgc aggcacagau     1620

ggacccugca aaguaccagc acaaauggcc guagacaugc aaacacuaac acccgucgga     1680

agacucauca ccgcaaaccc agucaucacg gaaucaacag aaaacagcaa aaugauguua     1740

gaacuggacc cacccuucgg cgacucauac auaguaauag gcgucggaga caagaaaauc     1800

acgcaucacu ggcaccgcag cggcagcaca auuggcaagg cauucgaagc aacagucaga     1860

ggagcgaaac gaauggcagu acucggcgau acagccuggg acuuuggauc cgugggaggc     1920

guguucaaca gccucggaaa agguauccac caaaucuucg gcgcagcguu caaaucacua     1980

uucggcggca ugagcugguu cagccaaaua cucaucggaa cacuccuagu auggcuagga     2040

cucaacacca aaaacggcag caucagccug acaugccuag cgcucggagg agucaugaua     2100

uuccucucaa ccgcaguguc ggccgacgua gggugcagug ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5109
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 1 opt

<400>  5109
gggagaaagc uuaccaugag aauaaucaac gcccgcaaag agcgcaaaag aaggggagca       60

gacacgagca uaggcaucau cggacuccug cucaccaccg caauggcagc agaaaucacc      120

agaagaggau cagccuacua cauguaccug gacagaagcg acgcgggaaa agcaauaucc      180

uucgcaacga aucuaggcgu caacaaaugc cacguacaga ucauggaccu aggucauaug      240

ugcgacgcca ccaugucaua cgaaugccca augcuagacg aaggcgucga accagacgac      300

gucgacugcu ggugcaacac cacguccacc ugggugguau acggcacaug ccaccacaag      360

aaaggcgaag cccgaaggag cagacgcgcu gucacccuac caagccacag uacgcgcaag      420

cuccaaacaa gaagccagac cuggcuagaa agccgcgaau acacaaagca ccuaaucaaa      480

gucgaaaacu ggaucuuccg aaacccagga uucgcccucg cagccgucgc aaucgcaugg      540

cugcuaggaa gcuccaccag ccaaaaggua aucuaccugg ucaugauccu acugaucgca      600

cccgccuacu cgauucgaug caucggcgua ucgaaccgcg acuucgucga agguaugagc      660

ggcggaaccu gggucgacgu cguacuagaa cauggcggcu gcguuacagu aauggcacaa      720

gauaagccca ccguagacau cgaacucguc accacgaccg uaagcaacau ggcagaaguc      780

cgcucuuacu gcuacgaagc aagcaucagc gacauggcaa gcgacucacg auguccaacc      840

caaggggaag cauaccucga caagcaaagc gauacacaau acguaugcaa aaggacccuc      900

gucgaccgag gcuggggaaa cggaugcgga cucuucggca aaggaagccu aguaacaugc      960

gccaaauuua caugcagcaa gaaaaugaca ggcaaaagua uccagccaga aaaccucgaa     1020

uaccgcauca ugcuuagcgu acacggcagc caacacucag gaaugaucgu caacgacaua     1080

ggacacgaaa ccgacgaaaa cagggcgaaa guagaaguca cacccaacuc cccacgagca     1140

gaagcuaccc ugggcggauu cgggagccug ggccucgacu gugaaccgag aacaggacua     1200

gacuucagcg accucuacua ccucacaaug aacaacaaac acuggcuggu gcacaaagaa     1260

ugguuccaug acauaccgcu cccauggcac gcaggagccg acacaggaac accgcacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg cgaaaaggca gacagucguc     1380

guccugggau cacaggaagg cgcaguacac acagcacucg ccggcgcguu agaagcagag     1440

auggacggug ccaaaggacg acuguuuuca gggcaccuca aaugccggcu aaaaauggac     1500

aaacugcgcc ucaaaggagu aucauacagc uuaugcaccg ccgccuuuac cuucaccaaa     1560

guccccgcag aaacccucca cggcaccgua acaguggaag uacaauacgc agggaccgac     1620

gggccaugca agguaccugc acaaauggcc gucgacaugc aaacacucac acccgucgga     1680

agacuaauca ccgccaaccc aguaauaaca gaaagcacag aaaacagcaa aaugaugcua     1740

gaacuagacc caccauuugg agacagcuac aucgugaucg gagugggcga caagaaaaua     1800

acccaucacu ggcaccgcuc aggcagcaca auaggcaaag ccuucgaagc aacagucaga     1860

ggagcgaaaa gaauggccgu acugggagac acugccuggg acuucggcuc cgucggcggg     1920

guauucaaca gccuuggcaa aggcgugcac cagauauucg gggccgcguu caaaaguuua     1980

uucgggggaa ugaguugguu cucccaaaua cuaauaggca cacuucuagu auggcucgga     2040

cuaaauacga aaaacggaag cauuagccuc acaugcuuag cccucggcgg aguuaugaua     2100

uuccucagua cagcaguguc agccgacgug ggaugcuccg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5110
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 1 opt

<400>  5110
gggagaaagc uuaccaugcg caucauaaac gcacgcaaag aaaggaaacg cagaggagcg       60

gacaccucca uaggaauaau aggccuacua cucacaaccg ccauggcggc cgaaaucacg      120

cgacgaggau cugcguacua cauguaccug gaccgcagcg augccggaaa agccaucucc      180

uucgcaacga cgcucggagu caacaagugc cauguacaaa uaauggaccu cggucacaug      240

ugcgacgcca cgaugucaua cgaaugcccg augcucgacg aaggcguaga accagacgac      300

gucgauugcu ggugcaacac aacgagcaca ugggucguau acggcacuug ccaccacaaa      360

aagggagaag cacgccgcag ccgccgagca gucacacugc caucccacag cacgcgcaaa      420

cuacaaacca ggagccaaac guggcuagaa agcagagaau acacgaaaca ccugaucaaa      480

gucgaaaacu ggauauuccg aaaccccgga uucgcccugg uagcaguagc aaucgcaugg      540

cuacuaggcu ccagcacauc ccaaaagguc auauaccuag uaaugauacu acucaucgca      600

ccagccuaca gcauaagaug caucggcgua agcaaccgcg acuucgucga aggaaugucc      660

ggcgggaccu ggguugacgu aguacucgaa cacggcggau gcgucaccgu gauggcacaa      720

gacaaaccca cgguagacau cgaacucguc acaacgacag ucagcaacau ggcggaagug      780

cguucguacu gcuacgaagc cagcauauca gacauggcau ccgacucaag augcccaaca      840

caaggcgaag ccuacuuaga caaacaauca gacacccaau acgucugcaa acggacacuu      900

gucgaccgcg gauggggaaa cggaugcgga cuauucggaa aaggcucgcu agugacaugc      960

gcaaaguuca caugcucaaa gaaaaugaca ggcaaaucca uacaaccaga aaaccuugaa     1020

uaucgaauua ugcucagcgu ccacggcucc caacacucag gaaugauagu caacgacaua     1080

ggacacgaaa cagacgaaaa cagagccaaa guggagguca caccaaacuc ccccagagca     1140

gaagcaacac uaggaggauu cggaucccug ggacuagacu gcgaacccag gacaggccuu     1200

gacuucuccg accucuacua ccucaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccccu gcccuggcau gccggggcag acacagggac accccacugg     1320

aacaacaaag aggcucuggu agaguucaaa gacgcgcacg cgaaaagaca aacagucguc     1380

guccucggcu cccaggaagg cgccguacau acagcacucg cuggcgcgcu agaagccgaa     1440

auggacggcg caaaaggcag acucuucucc ggacaccuaa aaugccgacu caaaauggac     1500

aaacuacgac ugaaaggcgu auccuacagc cuauguacag cagcauucac cuucacuaaa     1560

guaccagccg aaacccuaca cggcacagua accguagaag uccaguacgc aggaacagac     1620

ggccccugca aaguaccagc acagauggca gucgacaugc aaacgcugac cccgguaggg     1680

aggcucauaa ccgccaaccc aguuaucacg gaaucaaccg aaaacagcaa aaugaugcuc     1740

gaacuggacc cacccuucgg cgauucauac auaguaauag gaguaggcga caagaaaaua     1800

acccaccacu ggcacagauc cggcagcacc auaggcaaag ccuucgaggc cacaguacgc     1860

ggcgccaaaa gaauggccgu acucggagac accgcauggg acuucggauc cgucggaggc     1920

guauucaaca gccucggcaa aggaauccac caaauauucg gagccgccuu caaaagccua     1980

uucggaggca ugagcugguu cagccaaauc cuaaucggga cgcuccuagu auggcuaggc     2040

cucaacacca aaaacggaag caucagccua accugucuag cccugggcgg cguaaugauc     2100

uuccuaagca ccgcggucag cgccgacgua ggcugcagcg uugacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5111
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 1 opt

<400>  5111
gggagaaagc uuaccaugag aauaaucaac gcaaggaagg aaaggaaaag gaggggcgca       60

gacacgagca uagggauaau cggccuacua cugacaacag cgauggccgc cgaaauaacc      120

aggaggggca gugcauacua cauguaccug gacagaagcg acgccgggaa agcgaucucg      180

uucgcgacua accucggcgu caacaagugc cacgugcaaa uaauggaccu cggccacaug      240

ugcgacgcaa ccaugucgua cgaaugcccg augcuagacg aaggagugga acccgacgac      300

guagacugcu ggugcaacac aacgagcacc uggguaguau acggaaccug ccaccacaag      360

aaaggcgaag cgcgccggag ccggcgagcg gucacacucc caagccauag cacacgaaaa      420

cuccagaccc gcagccagac auggcuagaa agcagagaau acacaaagca ccugaucaaa      480

gucgaaaacu ggauauucag aaacccaggg uucgcacuag cagcgguagc caucgccugg      540

cugcuaggca gcuccacuag ccagaagguc auauaccucg uaaugaucuu acucaucgcg      600

ccagcauacu caauaaggug caucggcgug agcaaccgcg acuucgucga aggcaugucg      660

ggaggaaccu gggucgacgu cguccuagaa cacggcggcu gcgucacagu uauggcacag      720

gacaagccca caguagacau agaacucguc acaacaaccg ugagcaacau ggccgaagua      780

cgaagcuacu gcuaugaagc gucaaucuca gacauggcau cagacagccg augccccaca      840

cagggggaag cauaccucga caagcaaagc gacacccaau acguauguaa acgaacccuc      900

gucgacagag gaugggggaa cggauguggc cuauucggaa aaggcagccu cgugaccugc      960

gcaaaauuua ccugcucaaa gaaaaugacg ggaaagagca uccaacccga aaacuuagaa     1020

uacagaauca ugcuauccgu ccauggaucc caacacagcg gcaugauagu caacgacgaa     1080

aacagagcca aaguggaggu cacgccaaac agcccaagag ccgaagccac ccucggcgga     1140

uucggcagcu ugggccuaga uugcgaaccc aggaccggac uagacuucuc cgaucuuuau     1200

uaccugacca ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauccca     1260

cuaccauggc acgcaggagc agacacaggg accccgcacu ggaauaauaa agaagccuug     1320

gucgaguuca aagacgcaca cgcgaaacgc caaaccguag uaguacuagg cucccaagaa     1380

ggugcggucc acaccgcucu agccggagcc cucgaagcag aaauggacgg cgccaaaggc     1440

cgacuguucu caggccaccu aaaaugucga cuaaaaaugg acaaauuacg ccucaaaggc     1500

guaaguuacu cgcucugcac agcggcauuc accuucacca aagugccagc cgaaacuuua     1560

cacggaaccg uaaccgucga aguacaauau gcgggaacag acggacccug caaaguucca     1620

gcucaaaugg cggucgacau gcagacgcuc acccccgucg gccgacucau aaccgccaac     1680

cccgucauaa cugaaagcac agaaaacuca aagaugaugu uagaauuaga cccacccuuc     1740

ggcgacucau acaucguaau aggcguagga gacaagaaaa uaacccacca cuggcaccga     1800

ucagguagca cgauagguaa agcauucgaa gccacaguca gaggcgcaaa acgcauggca     1860

gugcugggcg acacagcgug ggacuucgga uccgucggcg gaguauucaa cucacucggg     1920

aaagggaucc accaaauauu cggagccgca uucaaauccc uauucggcgg aaugagcugg     1980

uucucccaaa uacuaauagg caccuuauug guauggcuag ggcucaacac caaaaacgga     2040

agcauaagcc ugaccugccu agcacucgga ggagucauga uauuccucag caccgcaguc     2100

agcgcagacg ucggaugcag cguggacuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  5112
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 1 opt

<400>  5112
gggagaaagc uuaccaugag aauuaucaac gcccgaaaag aacgcaagcg gcgaggcgca       60

gacaccucaa uaggaaucgu cggccuacua cuuaccacag cgauggcggc agaaaucaca      120

cgacgcggca gugcauacua uauguaccua gaccgcucag acgccggcaa agcaaucagu      180

uucgcaacca cccucggcgu caacaagugc cacguccaaa uuauggaccu aggccacaug      240

ugcgacgcca ccauguccua cgaaugcccc augcuugacg aaggagucga acccgacgac      300

gucgacuguu ggugcaacac cacaagcacc ugggucgucu acggcacaug ucaccacaag      360

aaaggcgaag cgcgaaggag cagacgagcg guaacccuac caucccauuc cacccguaaa      420

cuucaaacac gcagccaaac cuggcucgaa agccgagaau acacaaaaca ccuaaucaaa      480

gucgaaaauu ggauauuccg caacccgggg uucgcucuag ucgccguggc cauagccugg      540

cuccuaggcu ccucaacguc acaaaaggua auauaccucg ucaugauacu ccuaaucgca      600

ccagcguaca gcauccgaug cauaggcguc agcaaccgcg acuucgugga ggggaugagc      660

ggaggcaccu ggguagaugu cguacucgaa cauggaggcu gcgucacagu cauggcacaa      720

gacaagccaa caguggacau agaacucguc acaacgacgg uuagcaacau ggccgaggua      780

cgaagcuacu gcuacgaggc cagcauauca gacauggcca gcgacucaag gugccccacc      840

caaggggaag cauacuugga caaacaaucc gacacccaau acgucugcaa acggacccug      900

guggaccgag gguggggcaa cggcugcggc cucuucggaa aagguucccu cguuacaugc      960

gccaaauuua cgugcagcaa gaaaaugacc ggaaaaucaa uacaaccuga aaaccucgaa     1020

uacagaauaa ugcucagcgu ccacggcucg caacacagcg gaaugaucgu aaacgacacc     1080

ggacacgaaa ccgacgaaaa ccgcgccaaa gucgaaguca caccaaacuc cccacgcgcc     1140

gaagccacac ucggcggauu cgguagccua ggacuggacu gcgaaccacg cacaggacua     1200

gacuucuccg accucuacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccccu cccauggcau gcgggcgccg acaccggcac accccacugg     1320

aacaacaaag aagcucuagu agaguucaaa gacgcacacg cuaaacgcca aaccgucgua     1380

guccuuggcu cccaagaagg cgcuguacau accgcccuag cuggugcccu cgaagcagaa     1440

auggacggag ccaaaggacg ccuguucucc ggccaccuca aaugccgccu caaaauggac     1500

aaacuccgcc ucaaaggagu aucauacucc cuaugcaccg cagcuuucac guucacaaaa     1560

gugccagccg aaacacuaca cggaacagug accgucgaag uccaauacgc cggcaccgac     1620

ggacccugca aaguacccgc ccagauggcc guagacaugc agacccuuac ccccgucgga     1680

agacuaauca ccgcaaaccc agucaucacc gaaucaacag aaaauagcaa aaugaugcuc     1740

gaauuagacc cacccuucgg cgacucauac aucgucauug gcguaggcga caagaaaaua     1800

acccaccacu ggcacagguc agguuccacc aucggcaaag cauucgaagc caccguacga     1860

ggcgccaaac gcauggcagu ccuaggagac accgcguggg acuucggcag cgugggagga     1920

guuuucaacu cccucggaaa aggaauccac caaaucuuug gcgcggcauu uaaaagucua     1980

uuuggaggaa ugagcugguu cucacaaauu cugauaggca cccuccucgu cuggcuaggu     2040

cuaaacacca aaaacggcag uaucucacua accugccucg cacuaggagg gguaaugauc     2100

uuccucagua ccgcagucuc cgcagacguu gggugcagcg ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5113
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 1 opt

<400>  5113
gggagaaagc uuaccaugcg aauaaucaac gcgaggaaag aacgaaagag acggggcgcg       60

gacacaucca uaggcauagu agggcugcug cuaacaacgg cgauggcagc cgaaauaaca      120

agaaggggaa gcgccuacua cauguaucuc gaccgaucgg acgccgggaa agcaaucagc      180

uucgcaacca cccucggagu uaacaaaugc cacguacaaa ucauggaccu aggccacaug      240

ugcgacgcca caaugagcua cgaaugccca augcuggacg aaggcgugga acccgacgac      300

gucgacugcu ggugcaacac gacaagcacc uggguggucu acggcacaug ccagcacaag      360

aagggggaag cccgacgauc cagacgcgcg gucacacugc caagccacuc cacccgcaaa      420

cuacagacaa ggagccaaac cuggcucgaa agccgagaau acaccaaaca ucucauaaaa      480

guagagaacu ggauauuccg aaacccuggg uucgcccucg ucgcgguggc aaucgcaugg      540

cuacuaggau ccagcacgag ccaaaaaguc auauaccuag uaaugauacu cuuaaucgcg      600

ccagcauacu caauccgaug cauaggcguc uccaacagag auuucgucga aggaaugucc      660

ggcggcaccu ggguagacgu cgugcuagaa cauggaggcu gcguaacagu aauggcacaa      720

gacaaaccaa cagucgacau cgagcucgug acaacaaccg uaaguaacau ggcggaagug      780

cgcucguacu guuacgaagc cagcaucucu gacauggcaa gcgauagcag augcccaacg      840

caaggcgaag cguaccucga caagcaaucc gacacacagu auguaugcaa gaggacauug      900

guagacagag guugggguaa cggaugcgga cuguucggaa aaggcucgcu aguaaccugc      960

gccaaauuca caugcaguaa gaaaaugaca ggcaaaagca uacagcccga gaaccucgaa     1020

uacaggauaa ugcucucagu gcacggaagc caacacagcg gaaugauagu aaacgacauu     1080

ggccacgaaa cugacgagaa cagagcaaaa guagaaguga cacccaacag cccaagggcg     1140

gaggcaacac uaggcggauu cggcagccua ggccuagacu gugaaccgcg aacaggccuc     1200

gacuucagcg accuauacua cuuaaccaug aacaacaaac acugguuggu gcauaaagag     1260

ugguuccacg acauaccacu uccauggcac gccggagcag acacagggac cccacacugg     1320

aacaacaaag aagcacuggu cgaguucaaa gacgcgcacg ccaaacgaca gacaguaguc     1380

guccugggaa gucaagaagg cgcgguacac accgcacucg caggcgcccu agaagccgaa     1440

auggacggcg ccaaaggaag gcuguucagu ggccaccuaa aaugcaggcu caaaauggac     1500

aaacuucgac ugaaaggggu aagcuacucg uugugcaccg ccguaugcac agccgccaaa     1560

guacccgcgg aaacacuaca cggcacggug acaguggaag uccaguaugc cgggaccgac     1620

ggacccugca aggucccagc ccaaauggca guagauaugc aaacccucac gcccguaggg     1680

cgacuaauaa ccgcaaaccc aguaauaaca gaaucgacag aaaacagcaa aaugaugcug     1740

gaacucgacc cgcccuucgg agacagcuau auaguaauag gggucggaga caagaaaaua     1800

acacaccacu ggcaccggag cggaagcaca auugggaaag cauucgaagc gacagugcgg     1860

ggagcuaaaa ggauggcggu acuaggagac accgcauggg auuucgggag cgucggcggg     1920

guauucaaca gccuagggaa aggcauacau caaauauuug gcgcagcuuu caagagccua     1980

uucgggggca ugagcugguu cucacaaaua cuaauaggaa cccuccuagu cuggcucggg     2040

cucaacacaa agaauggauc aauaagccua acaugccuug cucucggagg cgugaugaua     2100

uuccuaucaa cagcggugag cgcggacgug ggaugcuccg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5114
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 1 opt

<400>  5114
gggagaaagc uuaccaugcg aauaauaaac gcccgcaagg aaagaaaacg caggggcgcc       60

gacgccucaa uaggaaucgu cucacuccua cucacaacag ugauggccgc agagaucacg      120

cgcagaggau ccgcauacua cauguaccua gacagaagcg acgcaggaaa ggcaauaucc      180

uucgucacaa cccucggcgu gaacaaaugc cacguccaaa uaauggaccu aggccacaug      240

ugcgacgcca caaugucaua cgagugccca augcuggacg aaggcgucga accggacgac      300

gucgacugcu ggugcaacac aacguccaca uggguagucu acggaaccug ccaccacaag      360

aaaggcgaag cacggaggag ccggcgcgca guaacauuac caucacacag uacccgaaaa      420

cuccaaacca gaucccaaac cuggcuagag agccgcgaau acaccaaaca ccuaaucaaa      480

guggaaaacu ggauauucag aaaucccgga uucgcacucg ucgccguagc caucgcaugg      540

cuacugggau ccagcacaag ccaaaaaguu aucuaccucg uaaugauacu ccugaucgca      600

cccgcauacu caauaagaug cauaggcgua agcaaccgcg acuuuguaga aggaaugagc      660

ggagggacgu gggucgacgu cguacuagag cacggaggau gcgucaccgu gauggcccag      720

gacaaaccca ccgucgacau ugaacucguc acgaccacag uaagcaacau ggccgaagua      780

cggucauacu gcuacgaagc cucaauaagc gacauggcau cagacucacg cugcccaaca      840

caaggggaag ccuaccuaga uaagcaaucc gacacccaau acguaugcaa acgcacccua      900

gucgaccgcg gaugggggaa cggaugcgga cuauucggca aaggaucgcu ggugaccugc      960

gccaaauuca caugcucaaa gaaaaugacc gggaaaagca uccaaccaga aaaccucgaa     1020

uaccguauaa ugcuaucagu ccacggcucu caacauucag gcaugaucgu caacgacgag     1080

aaccgcgcaa aaguagaggu gacccccaac uccccaagag cagaggcgac ccucggagga     1140

uucggcucac ugggccuaga cugcgaaccc cgcaccggcu uagacuucag cgaccucuac     1200

uaccuaacca ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauacca     1260

cuucccuggc auucaggggc cgauaccgaa accccgcacu ggaacaauaa agaagcccuc     1320

guagaguuca aggacgcgca cgccaagaga cagacagucg uaguccuugg aagccaggaa     1380

ggcgccguac acacugcccu cgccggagca uuagaagccg aaauggacgg cgcgaaaggc     1440

cgacuaucau cuggccaccu gaaaugccgg cucaagaugg acaagcugcg ucucaaggga     1500

gugucauacu cacucugcac agccgcauuc acauucacca agguccccgc ugaaacccuc     1560

cacggaaccg ugaccgucga aguccaauac gccggacgag acggaccuug caagguuccc     1620

gcccagaugg ccgucgacau gcaaacccua acacccgucg gcagacuaau caccgccaac     1680

cccgucauca cagaaucaac agaaaacuca aagaugaugc uagaacuaga cccaccauuc     1740

ggggacagcu acauaguaau aggcgucgga gacaagaaaa ucacgcacca cuggcaccgc     1800

agugggucaa uaauaggcaa agcguucgaa gccacggucc gcggagccaa acgcauggca     1860

guacuaggcg acaccgccug ggacuucgga agcgucggag gcguguucaa cucacucggc     1920

aaaggaauac aucaaauauu cggcgcugcc uucaaaucuc uauucggagg uauguccugg     1980

uucucacaaa uacuaaucgg aacccuccuc gucuggcucg gacuuaacac caaaaacggg     2040

agcaucucac ugaccugccu cgcccucggc ggcguaauga ucuuccuaag cacagcagua     2100

uccgcagacg ucggcugcag cguagacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  5115
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 1 opt

<400>  5115
gggagaaagc uuaccaugcg caucauaaac gcccgcaaag aaagaaaaag aagaggagcc       60

gacaccagua ucggaauagu uggacuccuc cuaacaaccg ccauggccgc cgaaauaacc      120

cgccgcggca gcgcuuacua cauguaccuc gacaggagcg augcaggcaa agccaucuca      180

uucgccacaa cuuugggcgu aaacaaaugc cacgugcaaa ucauggaccu cggccacaug      240

ugcgacgcca cuauguccua cgaaugcccc augcucgacg aaggcguaga accugacgac      300

guagacugcu ggugcaauac aaccagcacc uggguagucu auggaacaug ccaccacaag      360

aaaggugaag cacgccgauc acggagagcc guaacccuac ccucucacag cacccgcaaa      420

cuacaaaccc gcucccaaac auggcuagag ucacgcgagu acaccaaaca cuuaaucaaa      480

guggaaaacu ggaucuuccg uaaccccggc uucgcccucg ucgcagucgc cauagccugg      540

cuccucggcu cauccacauc ccaaaaaguc auauaccuag uaaugauacu ccuuauugca      600

ccagccuaca gcaucagaug caucggcguc ucaaaccgag auuucgucga gggcaugucu      660

ggaggaaccu gggucgacgu aguccucgaa cacggaggcu gcgucaccgu aauggcccaa      720

gacaaaccaa caguugacau agagcuagug accaccacag ugagcaauau ggccgaaguu      780

cgauccuacu gcuacgaagc cagcaucuca gacauggcca gugacucucg cugccccacc      840

caaggagagg ccuacuuaga caaacaaagc gacacacaau acgucugcaa gcgcacccuc      900

gucgaccgcg gauggggaaa cgguugcgga uuauucggca aaggcucucu cguuaccugc      960

gcgaaauuca ccuguuccaa gaaaaugacc ggcaaaagca uccaacccga aaaccuagaa     1020

uacaggauaa ugcucagcgu ccacggcucg caacacuccg gaaugaucgu caacgaugaa     1080

aaccgggcca aagucgaagu cacgccgaac ucaccaagag cugaagcuac acuaggcggc     1140

uucggaagcc ucggccugga cugcgaaccc cgcacaggcc ucgacuucuc agaccucuac     1200

uaccucacca ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauaccc     1260

cucccauggc acgcaggcgc agacacaggc acaccccacu ggaacaacaa agaagcacuc     1320

guggaguuca aagacgccca cgcaaaacgg caaacagucg ucguacuagg cagccaagaa     1380

ggcgcugucc acaccgcacu ugcaggcgcc cucgaagccg aaauggacgg agcuaaaggc     1440

agacucuuca gcggccaccu aaaaugccgc cucaagaugg acaaacugcg acuuaaaggc     1500

gucucguacu cccuaugcac agcagccuuc accuucacaa aggucccggc ggaaacacua     1560

cacgggaccg uaaccgucga aguacaauac gcaggaaccg acggccccug caaaguaccc     1620

gcacaaaugg cggucgacau gcaaacacug acccccgucg gacgccugau aaccgccaac     1680

cccgucauaa ccgagucaac agaaaacagc aaaaugaugc ucgaacuaga cccucccuuc     1740

ggcgauucuu acaucgucau aggagucggc gacaagaaaa ucacacacca cuggcacaga     1800

uccgguucaa caaucggcaa agcauucgag gccaccgucc gcggcgccaa acgcauggca     1860

gucuuaggcg acaccgccug ggacuucggc agcguaggcg gaguauucaa cagucucggc     1920

aaaggcaucc accaaauauu uggcgccgcg uucaaaagcc uguucggggg cauguccugg     1980

uucucccaaa uccucauagg aacccuccua guauggcuug gacuaaacac caaaaacggc     2040

uccauauccc uaaccugccu cgcucucggc ggggugauga uauuccucag cacagcagug     2100

uccgccgacg ucggcugcuc cguugacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  5116
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 1 opt

<400>  5116
gggagaaagc uuaccaugcg aaucauaaac gcaagaaaag aaagaaaaag gagaggcgcc       60

gacaccucaa ucgguauaau cggccuacuc cucacaacgg ccauggccgc agaaaucaca      120

agacgaggcu ccgcauacua cauguaccuc gaccguucag acgcgggcaa agcaauauca      180

uucgccacaa cccucggagu aaacaaaugc cacguccaaa uaauggaccu aggacacaug      240

ugcgacgcca cuaugagcua cgaaugccca augcuagacg aaggagucga acccgacgac      300

gucgacugcu ggugcaacac caccucgacc uggguagugu acgggaccug ccaccacaaa      360

aagggggaaa cccgaagauc ccggaggagu gugagccuaa gguaccacua cacacgaaaa      420

cuccagacca gaagccaaac cuggcuggaa uccagagaau acaagaaaca ccucauaaug      480

guagaaaacu ggauauucag aaacccagga uucgcgauag ucagcguggc aaucacaugg      540

cuaaugggaa gccucaccuc ccaaaaagua auauacuuag ugaugaucgu ccucauagua      600

ccagccuaca gcaucagcug caucggaguc agcaaccgcg acuuagucga ggggaugucg      660

ggcgggacau gggucgacgu aguacucgaa cauggaggcu gcguaacaga aauggcacaa      720

gacaaaccca ccguagacau cgagcucgua accaugacag ucucaaacau ggccgagguc      780

cggucguacu gcuacgaggc gucccuguca gauauggcaa gcgccuccag augcccuacc      840

caaggcgaac caagucucga caaacaauca gacacccaaa gcguaugcaa aagaacacuc      900

ggagacaggg guuggggaaa uggcugugga auauucggaa agggaagccu aguaacaugc      960

aguaaguuua ccugcugcaa gaagaugcca ggaaaaucaa uacaaccaga aaaccuggaa     1020

uaccgaauaa ugcuccccgu acacgguuca cagcacagcg gcaugaucgu aaacgacauu     1080

ggacacgaaa cagaugaaaa cagggccaaa guggaaguga caccgaacag cccacgcgca     1140

gaagccaccc ucggaggcuu cggcucacuc ggacuggacu gcgaaccacg uacggggcuu     1200

gacuucagcg accucuacua ccugacgaug aacaacaaac acuggcuagu acacaaagag     1260

ugguuccaug acauaccccu acccuggcac gccggcgcag auacaggcac gccucacugg     1320

aacaacaagg aggcacucgu cgaguucaaa gacgcccacg caaaaaggca aaccguaguc     1380

gugcucggcu cccaggaagg agcaguacau acagcacugg ccggagcccu agaagcagaa     1440

auggacggag ccaaaggacg ccucuucuca ggccaccuga aaugcaggcu caaaauggac     1500

aaacugagac ucaagggcgu aagcuacagc cucugcacag cagccuucac auucaccaaa     1560

gugcccgcag aaacacucca cggaacgguc accgucgaag uacaauccgc cggaacagac     1620

ggccccugca aaguacccgc acaaauggcu gucgacaugc aaacauuaac acccgucgga     1680

cgacugauca ccgcaaaucc gguaaucacc gaauccacag aaaauagcaa aaugaugcuc     1740

gaacucgacc cacccuucgg agacagcuac auagucaucg gcgucgguga caaaaagauc     1800

acccaccacu ggcacaggag cggcagcaca auaggaaaag ccuucgaagc aaccgugcga     1860

ggagcgaaaa gaauggcagu guuaggcgac accgccuggg acuucggcuc aguaggagga     1920

guguucaaca gccuaggaaa aggaauacac caaaucuucg gcgcggcauu caaaagucuu     1980

uucggaggaa ugagcugguu cagccagaua uuaauaggca cccuccucgu auggcuaggu     2040

cuaaacacca aaaacggcuc caucucacua accugccucg cgcuaggcgg agucaugauc     2100

uuccucagca cagccgucuc cgcagacgua gggugcucag ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  5117
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 1 opt

<400>  5117
gggagaaagc uuaccaugcg cauaauaaac gcccgaaaag aacgcaaacg aagaggcgcc       60

gacacaucaa uaggaauaau aggccuccuc uuaacgacag cgauggcagc cgaaauaacc      120

aggagggguu cagccuauua cauguaccug gaccgcagcg acgcaggaaa agccaucucg      180

uucgcaacaa ccaugggagu caacaagugc cacguacaaa ucauggaccu aggacacaug      240

ugcgacgcca caauguccua cgaaugcccu augcuagacg aaggcguaga acccgacgac      300

gucgacugcu ggugcaacac caccagcaca ugggugguau acggaaccug ccaccacaag      360

aaaggagaag cgagaccguc cagacgagcg guaacacugc cgagccacuc cacccgcaaa      420

cuucaaacca ggucacaaac auggcucgaa agcagagaau auacaaagca ccuaaucaaa      480

gucgaaaacu ggauccuccg caacccagga uucacagugg cagcacuagc aauggccugg      540

cuacugggaa acagcaccuc acaaagaguc aucuaccuag ucaugauccu acuaaucgcc      600

cccgccuacu cgaucaggug caucggcguu agcaaccggg acuucguaga aggcaugagc      660

ggcggaacau gggucgacgu agugcucgaa cacggcgggu gcgucacagu aauggcacaa      720

gacaaaccca cagucgauau cgagcuaguc accacaacag uaagcaacau ggccgaaguc      780

aggucguacu gcuacgaagc aagcauaagc gacauggcaa gugacagcag augccccacc      840

caaggggaag ccuaccuaga caaacaaucc gacacccaau acgucugcaa gcgcacccua      900

gucgacagag gguggggaaa cggcugcggc cuauucggca aaggaucccu aguaacaugc      960

gcaaaauuua ccugcagcaa aaagaugaca ggcaaaucaa uccaacccga aaaccucgaa     1020

uacagaauca ugcucucagu ccacgguuca caacacucgg ggaugaucgu caacgauauc     1080

ggacacgaaa ccgaugaaaa cagagcaaag gucgaaguca cuccaaacuc cccuagagcc     1140

gaagcaaccc ucggaggauu cggcucccuc ggccuagacu gcgaaccacg caccgggcuc     1200

gacuucagcg accuauacua ccuaacaaug aacaacaaac acuggcuggu acacaaagaa     1260

ugguuccacg acauaccacu accuuggcac gcaggggccg acaccggcac accccacugg     1320

aacaacaaag aagcgcuagu agaguucaag gacgcacacg caaaaagaca aaccgucgua     1380

guacuaggaa gccaggaagg cgcgguccac accgcgcugg ccggcgcacu agaagccgaa     1440

auggauggag caaaagggcg ccucuucucc ggccaccuca aaugccggau caaaauggac     1500

aaguuaagac uaaaaggcgu cucauacagc cuuugcacag cagccuucac auucucgaaa     1560

gugccggcag agacacugca cggcaccgua accgucgaag uacaauaugc aggcagagac     1620

ggcccaugca agguacccgc acaaauggca gucgacaugc agacacuaac cccagucggc     1680

cgccuaauaa cagcaaaccc caucaucaca gaaagcacag aaaacucccg caugaugcuc     1740

gaacuagacc cacccuucgg cgacagcuac auaguaaucg gggucggcga caagaaaaua     1800

acacaccacu ggcaccgaag ugggagcaca aucggaaaag cauucgaagc cacagucaga     1860

ggagccaaac ggauggcagu ccucggcgac accgcauggg acuucggaag cgucggaggc     1920

gucuucaacu cauugggcaa aggaguccac caaguuuuug gggccgcauu caaaagccug     1980

uucggaggga ugagcugguu cucgcaaaua cuaaucggca cccugcucgu cuggcucggc     2040

cucaacacaa agaacggauc uaucucacuu gcaugccucg cacucggcgg cgccaugauc     2100

uuccuaucca cggcagucag cgccgacgua ggcuguagcg uagacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  5118
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 1 opt

<400>  5118
gggagaaagc uuaccaugac aucaguagga auaguaggcc ugcuacucac caccgcaaug       60

gccgcggaag ucaccagaag agggagcgca uacuacaugu accuagacag gaacgacgcg      120

ggagaggcca uaucauuccc aacuacgcua gguaugaaca aaugcuauau ccaaauaaug      180

gaccuagggc auaugugcga cgccacgaug aguuacgagu gccccaugcu agacgaaggg      240

gucgaaccgg acgacguaga cugcuggugc aacacaaccu caaccugggu agucuacggg      300

accugccacc acaagaaagg cgaagcacgc cgaucaagac gagcgguaac ccucccaagc      360

cacucgacaa gaaaacucca aacccgaagu caaaccuggc ucgaaucucg cgaauacacc      420

aaacaccuca uucgaguaga aaacuggauc uuccggaacc caggcuucgc guuagccgcc      480

gccgccauag cauggcuacu uggaagcucc accucccaaa aagucauaua ccuaguaaug      540

auccuccuca uagcgccagc cuacuccauu agaugcauag gcguaagcaa uagagacuuc      600

gucgaaggca ugagcggggg cacguggguu gacaucgucc uggagcacgg cggcugcgua      660

acaguaaugg cccaagacaa accaaccguu gacaucgagc uagugacgac aaccguauca      720

aacauggcag aaguaagguc cuacugcuac gaagcaagca ucuccgacau ggcaucagac      780

agcagaugcc cgacacaagg agaagcauac cuagacaagc aaagcgacac ccaguacgua      840

ugcaaaagaa cgcuggucga ccgcggaugg ggaaacggau gcggccuauu uggcaaagga      900

agccuaguaa ccugcgccaa auucgcaugc uccaaaaaga ugacaggcaa auccauccaa      960

ccagaaaacc uagaauaucg aaucauguua agcguacacg gaagucaaca cucaggcaug     1020

aucgucaacg acacaggaca cgagacagac gaaaacagag caaaagucga aaucacacca     1080

aauucacccc gagcagaagc cacccuaggg ggcuucggau cgcucggccu agacugugaa     1140

ccacgcaccg gacuggauuu cagcgaccua uacuaccuaa ccaugaauaa caagcacugg     1200

cucguacaca aagaaugguu ccacgacaua ccacucccau ggcacgccgg cgccgacaca     1260

gggacgccac auuggaacaa caaagaagca cucguagaau uuaaagacgc ccacgccaaa     1320

cgacaaacag ucguggucuu ggggucccaa gaaggcgcgg uccacacagc acucgccggc     1380

gcccuggaag cggaaaugga cggcgccaag ggcagacugu caucgggcca ccuaaaaugc     1440

cgacuaaaga uggacaaacu ccgccucaaa ggcguaucau acucccucug cacggcagca     1500

uucacauuca ccaaaauacc cgcggaaaca cuacacggca ccgucacagu agaaguccaa     1560

uacgccggaa cggacgggcc guguaagguc cccgcccaaa uggccgucga uaugcaaacc     1620

cugacuccag ucggccgacu aaucacagca aaccccguca uaacugaauc cacagagaac     1680

aguaagauga ugcucgagcu cgacccaccc uucggcgaca gcuacauagu aaucggaguc     1740

ggcgagaaga aaauaacaca ccacuggcac cgcagcggau ccaccauagg caaagccuuc     1800

gaagccaccg uccgaggugc caaacgcaug gcaguacugg gcgacacagc cugggacuuc     1860

ggcagcgucg gaggagcccu aaacagccug ggaaaaggca uacaccaaau cuucggagcc     1920

gccuucaaau cccuuuucgg aggcaugucc ugguucagcc aaauccugau cggcacccug     1980

cuuauguggc uaggccuaaa caccaaaaac ggauccauca gccuaaugug ccuggcccua     2040

ggcggugugc uaaucuuccu aagcacugcc guaucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5119
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 1 opt

<400>  5119
gggagaaagc uuaccaugac uucagucggc aucguaggcc uacuccucac caccgccaug       60

gcagccgaag uaacccgccg cggcuccgca uacuacaugu accucgacag gaacgacgcu      120

ggcgaagcca ucuccuuccc cacaacccuc ggaaugaaua aaugcuacau ccaaaucaug      180

gaccucgggc acacaugcga cgcaacuaug uccuaugaau gccccaugcu agacgaaggc      240

gucgaacccg augacgucga uugcuggugc aacacaaccu caaccugggu agucuacgga      300

accugccacc auaagaaagg cgaagcccgc agaucccgac gcgccguaac acuaccgucc      360

cacuccacgc gcaaacucca gacaaggagc cagacauggc uagaaagccg cgaauacacc      420

aaacaccuaa uaagaguaga gaacuggauc uuccgaaacc caggauucgc acucgcagca      480

gcagccaucg cauggcuacu cggauccagc accucccaaa aaguaauuua ccuggucaug      540

auccugcuaa uagcgcccgc auacagcauc aggugcauag gggucuccaa uagagacuuc      600

guagaaggca ugagcggcgg aacgugggua gacgucguac ucgaacacgg cggcugcguc      660

acuguaaugg cccaagacaa accuacgguu gacaucgaac ucguaacaac aaccgucuca      720

aacauggccg aaguacgauc cuacugcuac gaagccucaa uauccgacau ggccuccgac      780

agccggugcc ccacgcaagg cgaagcauac uuagacaaac aaagcgacac acaauacguc      840

ugcaaacgaa cacuggucga ccgcggcugg ggaaacggcu guggacuauu cggaaaaggc      900

ucacucguaa caugcgcaaa auucgccugu agcaagaaaa ugacaggaaa aagcauccaa      960

cccgaaaacc ucgaauaccg aaucaugcug ucaguccacg gaagccaaca uucaggaaug     1020

aucgucaacg acacaggaca cgaaacugac gaaaacagag ccaaggucga gauaacacca     1080

aacagcccac gugcagaagc cacccuagga ggguucgggu cacucggccu ugacugcgaa     1140

ccgcggacgg gacuagacuu cagcgaccuu uacuaccuca caaugaacaa caaacacugg     1200

cuaguucaua aagaaugguu ccacgauauc ccacugcccu ggcacgcagg cgccgacaca     1260

ggcacaccac acuggaacaa caaggaagcc cucgucgagu ucaaagacgc ccacgcgaaa     1320

agacaaaccg ucgucguacu aggcucacaa gagggcgccg uccacaccgc ccuagccggc     1380

gcccuagagg cagaaaugga cggcgcaaag ggccgccucu cauccgggca ccucaaaugc     1440

cgccucaaaa uggacaaacu ccgucuuaaa ggcgucucgu auucccuaug caccgccgcc     1500

uucacguuca ccaaaauacc ggcagaaacg cuccacggaa ccgucaccgu agaaguacag     1560

uaugccggca cggacggccc cugcaaaguc ccagcccaaa uggccgucga caugcagaca     1620

cucaccccag ucggcaggcu aaucacggcg aaucccguca ucacagaauc cacagaaaac     1680

ucaaaaauga ugcuagaacu agacccgcca uucggcgaca guuacaucgu caucggaguc     1740

ggcgaaaaga agaucacaca ucacuggcac agaagcggca gcacaauagg gaaggccuuu     1800

gaagcaaccg ucagaggugc caaaagaaug gcaguccucg gcgacaccgc cugggacuuc     1860

ggaucgguag gcggugcccu caacucccua ggaaagggca uccaccaaau cuucggugcc     1920

gcauucaaau cccuauucgg gggcauguca ugguucucac agauacuaau aggaacccuc     1980

cucauguggc uaggccuaaa cgccaaaaac ggcagcauaa gccugaugug ccuagcccuc     2040

ggcggcguac uaaucuuccu cagcacagcc gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5120
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 1 opt

<400>  5120
gggagaaagc uuaccaugac gagcauaggc auaauaggac ucuugcucac aaccgcaaug       60

gccgccgaaa ucacgcgacg aggcucagcc uacuacaugu accuagacag aagcgacgca      120

ggcaaagcca ucucguucgc aaccacacuc ggaguaaaca aaugccacgu acaaauaaug      180

gaucucggac acaugugcga ugcaacaaug uccuacgagu gccccauguu agacgaagga      240

guagaaccgg acgacgucga cugcuggugc aauaccacaa gcacgugggu aguauauggc      300

acgugccacc acaagaaagg cgaagcacgc agaagccggc gcgccgucac gcuacccagc      360

cauagcacca gaaagcucca gacacggucc caaaccuggc ucgagagccg cgaauauaca      420

aaacaccuga ucaaggucga aaacuggauc uuccggaauc cuggauucgc acucgucgcc      480

guugcaaucg cuuggcuccu aggcucgagc acgagccaaa aaguaaucua ccucguaaug      540

auacuacuaa ucgcgcccgc auauucuauc cgaugcauag gcgugagcaa ccgugacuuc      600

gucgaaggaa ugucgggcgg gacauggguc gauguaguac uagagcacgg gggaugcguc      660

acggucaugg cacaagacaa gcccaccguc gauaucgagc ucgucacgac cacagugagc      720

aacauggcug aagugcgcuc auacugcuac gaagccucca uaucagacau ggcauccgac      780

agcagaugcc cuacacaagg ugaagcauac cuggacaaac aaucagacac acaauacguc      840

ugcaaaagga cccuagucga ucgcggaugg gggaacgggu gcggacuauu cggcaaagga      900

agccuaguga ccugcgcuaa auuuaccugc ucgaagaaaa ugacaggaaa gagcauccaa      960

cccgaaaacc ucgaauacag aaucaugcuc ucaguccacg gcucucaaca uagcgggaug     1020

auaggcuacg aaacugacga ggacagagcc aaagucgagg ucacgcccaa cagcccgcgc     1080

gcagaagcua cacugggagg auucggcagc cuaggacuag acugcgaacc acgcacagga     1140

cucgacuuca gcgaccuaua cuaccugaca augaacaaca aacacuggcu aguccacaaa     1200

gaaugguucc acgauauacc gcucccgugg cacgccggcg ccgacaccgg uaccccacac     1260

uggaacaaca aagaagcacu cgucgaguuc aaagacgcac acgcaaaaag acaaacgguc     1320

guaguauuag gcucgcaaga aggcgcaguc cacacagccc uagcaggcgc gcuagaagca     1380

gaaauggacg gcgcgaaagg cagacuauuc ucaggccauc ucaagugccg ccuaaaaaug     1440

gacaaacucc gccuaaaagg gguauccuac ucacucugca cagcagcauu cacauucacc     1500

aaaguccccg cggaaacccu acauggcaca guaaccguag aagugcaaua ugccggaacg     1560

gauggccccu gcaagauccc gguacaaaug gcgguugaca ugcagacucu gaccccagua     1620

gggcgccuaa ucacagcaaa cccuguaauc acugaaucca cagaaaacuc aaaaaugaug     1680

cucgaacugg acccaccauu cggggacagc uacauaguaa uaggagucgg cgacaagaaa     1740

aucacccacc acuggcacag aagcgggagu acaaucggaa aagcguucga ggccacaguc     1800

aggggcgcaa aaagaauggc aguacuagga gacaccgcuu gggacuucgg cagcgugggc     1860

gggguauuca acucacucgg aaaaggcauc caccaaaucu ucggugcagc cuucaaaagc     1920

cuguucggag gaauguccug guucucccaa auucucaucg gaacgcuccu agucuggcuc     1980

ggacuaaaua ccaaaaacgg aucuaucagc uuaaccugcc ucgcgcuagg aggagugaug     2040

aucuuccugu ccacagcagu guccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  5121
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 1 opt

<400>  5121
gggagaaagc uuaccaugac cuccguaggc aucguaggcc uacuccugac gaccgccaug       60

gccgccgaag ucacccgccg cggcuccgca uacuacaugu accucgaccg aaacgacgcc      120

ggagaagcca ucagcuuucc cacuacccuc ggcaugaaca aaugcuacau ccaaaucaug      180

gaccucgguc acaugugcga cgcuacuaug agcuacgaau gucccaugcu agacgaggga      240

guugaacccg acgacgucga cugcuggugc aacacaacau caacaugggu cguauacggc      300

accugucacc acaagaaagg cgaagcaaga cguucuagac gagccguaac acuaccaucc      360

cauucgaccc gcaaauuaca aacccgcucc caaaccuggc uagaaucacg agaauacacc      420

aaacaccuca uccgcgucga gaacuggauc uucaggaacc cgggauucgc acuugcugcc      480

gcagcaauag cauggcuccu cggauccucc accucccaaa aaguuauaua ccucgucaug      540

auccuccuua ucgcccccgc auacucgauc cgcugcauag gugucucaaa ccgagacuuc      600

guggaaggca uguccggggg cacgugggua gacguggucu uagaacacgg cggcugcgua      660

accguaaugg cacaagacaa accaacagug gacaucgaac uggucaccac aacaguaucc      720

aacauggccg aaguccgcag cuauugcuac gaagcaagca uaucugauau ggcguccgac      780

ucacgcugcc ccacucaggg cgaagccuac cuugacaagc aauccgacac ccaauacguc      840

ugcaaacgca cacucgucga ccgaggcugg ggcaacggau gcggacuauu cggaaagggc      900

ucccuaguca caugcgccaa auucgccugc uccaagaaaa ugaccggcaa aucgauacaa      960

cccgaaaacc ucgaauaccg uauaaugcua uccguccacg gcucacaaca uucagggaug     1020

aucgucaacg acacaggcca cgaaaccgac gaaaaccgag ccaaggucga aauaaccccg     1080

aacagcccca gagccgaagc cacccuagga ggcuucggcu cccuaggccu agacugcgaa     1140

ccccgcaccg gccucgacuu cuccgacuug uacuaccuca cgaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacaua ccccucccau ggcacgccgg cgcagauacc     1260

ggaacucccc acuggaacaa caaagaagcc cuagucgagu ucaaagacgc ccacgccaaa     1320

cgccaaacag ucguaguccu aggaucccaa gaaggcgccg uucacacggc acucgccgga     1380

gcacuagaag ccgaaaugga cggcgcaaaa ggccgucuau ccucaggcca ccuuaaaugc     1440

cgccugaaga uggauaaacu ccggcucaaa ggagucuccu acucacugug caccgccgca     1500

uucacauuca ccaaaauccc agccgaaacc cuccacggca caguaaccgu ugaaguccaa     1560

uacgccggca ccgauggccc augcaaagua ccggcccaaa uggcaguaga caugcaaaca     1620

cucacacccg uaggacgccu cauaacggcc aacccaguaa uaaccgaauc cacagaaaac     1680

uccaaaauga ugcuagaacu agacccaccc uucggggacu ccuacaucgu caucggcgua     1740

ggcgaaaaga aaaucacaca ucacuggcac cgcucaggau caacaauagg aaaagccuuc     1800

gaagccacag uaagaggugc aaaacgcaug gcaguacuag gagacacagc augggacuuu     1860

gggucagucg gcggagcccu gaacucacuc ggaaaaggga uccaccaaau cuucggcgcc     1920

gcauucaaau cccuauucgg aggaaugagc ugguucagcc aaauccucau cgggacccua     1980

cuaauguggc ucggccucaa caccaaaaac ggcuccaucu cccuuaugug ucucgcucua     2040

ggcggcguac ucauauuccu cagcaccgcc gucucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5122
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 1 opt

<400>  5122
gggagaaagc uuaccaugac gagcaucggc aucauagguc uccuccuaac cacggccaug       60

gccgccgaaa ucacccgccg cggcuccgca uacuacaugu accuugaccg cucagaugcc      120

ggaaaagcaa ucucauucgc gacaacccuc ggaguaaaca aaugccacgu acaaaucaug      180

gaccugggcc auaugugcga cgccacgaug uccuacgaau gccccaugcu agacgaaggg      240

gucgaacccg augacguaga uugcuggugc aacacaacaa gcaccugggu cgucuacggc      300

acaugccacc acaagaaagg agaagcacgc cgaucacgcc gagccgucac cuuacccucu      360

cacucaaccc gaaaacuaca gacaagaucc cagaccuggc uugaaagccg cgaauacaca      420

aaacaccuca ucaaagucga aaacuggaua uuccggaacc ccggauucgc ccuaguagca      480

guagcaauag cauggcugcu cggauccuca acaucacaaa aaguaaucua ccucgucaug      540

auccugcuua ucgcacccgc cuacucaauc agaugcauag gagucagcaa ccgcgacuuc      600

gucgaaggga ugucaggagg cacguggguc gacguagucc uagaacacgg cggcugcguc      660

acaguaaugg cccaagacaa accuaccgua gacaucgagc ucguaaccac cacuguaucc      720

aauauggccg aaguccguuc auauugcuac gaagccucaa ucuccgacau ggcaagcgac      780

ucccgaugcc ccacccaagg agaagcguac cucgacaaac aauccgacac ccaguacguc      840

ugcaaacgaa cuuugguaga ccggggcugg ggcaacggau gcggucucuu cgguaaagga      900

uccuuaguaa ccugcgcaaa auuuaccugc agcaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaacc ucgaauaccg caucaugcuc ucaguccacg ggucccaaca cuccggaaug     1020

auagucaaug acacaggcua cgaaaccgac gaaaaccgcg ccaaaguaga agucacaccc     1080

aacagccccc gcgcagaggc cacccucggc ggcuucggcu cccuaggccu agacugugaa     1140

ccgcgaacag gccuggacuu cuccgaccuc uacuaccuua cuaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacauc ccucuaccau ggcacgccgg agcagacacc     1260

ggcacacccc auuggaacaa caaagaagcc cuuguugagu ucaaggacgc ccacgcgaaa     1320

cgacaaaccg ugguaguacu ugguucacaa gaaggcgcag uccacacagc ccuagccgga     1380

gcccucgaag cagagaugga cggagccaaa ggcaaacucu ucuccggcca ccuaaagugc     1440

agacuaaaaa uggacaaacu aagacuaaaa gggguaucau acagccuaug caccgccgcu     1500

uucaccuuca cgaaaguccc agccgaaacc cuacacggca ccguaaccgu cgaaguacaa     1560

uacgcaggaa ccgacggccc cugcaaaauc cccguccaga uggccguaga uaugcaaaca     1620

cuaacccccg ucggacgccu aauaaccgcc aacccaguca ucaccgaaag cacugaaaac     1680

uccaaaauga ugcuagaacu cgacccgcca uucggcgaca gcuacaucgu aaucggaguc     1740

ggggauaaaa agaucaccca ccacuggcac cgcucaggca gcacgauagg caaagccuuc     1800

gaagccacag uccgcggggc caaacguaug gcaguccuag gagacacggc cugggacuuc     1860

ggcuccgucg gcggagucuu caacucccua ggcaaaggca uccaccaaau auucggcgcg     1920

gcauucaaaa gccuauucgg cggcaugucc ugguucucac aaauccuaau aggcacccuc     1980

cuaguauggc uaggacuaaa caccaaaaac ggauccauaa gccuaaccug ccuagcacua     2040

ggcggcguaa ugauuuuccu aagcacggcc gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5123
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 1 opt

<400>  5123
gggagaaagc uuaccaugac gaguaucggu aucauaggcc uacugcugac aaccgcgaug       60

gcugcagaga ucacacguag aggaagcgca uacuacaugu accuggaccg cagcgacgcc      120

ggaaaagcaa uaucauucgc cacaacgcuc ggaguaaaca aaugccacgu ccagauaaug      180

gaccucgguc acaugugcga cgcaaccaug ucguacgaau gccccaugcu agacgaggga      240

gucgaaccag acgacgucga uugcuggugc aacacaacca gcacgugggu cguguacgga      300

accugccaca gaaagaaagg ggaagcgaga agaagcagac gagcggucac acucccuagc      360

cacagcacac gcaaacucca aacccgaagc caaacguggc ucgaaagccg agaauacaca      420

aaacaccuca ucaaagucga aaacuggauc uuccgcaacc ccggguucgc acucguggca      480

gucgccauag cauggcuccu gggcagcagc acgagccaaa aaguaaucua ccuagugaug      540

auacuccuga ucgccccagc cuacucgaua cgaugcauag gcgucagcaa ccgcgauuuc      600

guggaaggaa uguccggcgg gacgugggua gacguagucc uggaacacgg aggcugcguc      660

accguaaugg cccaggacaa acccacaguc gauauagagc uggugacgac aacaguaagc      720

aacauggcag aaguacgcuc guacugcuau gaagcaucga ucucggacau ggcaagcgac      780

agccgcuguc caacccaagg agaggccuac cuagacaaac agagcgacac acaguauguc      840

ugcaaaagaa cccucguaga cagaggcugg ggaaacggau gcggccucuu cggcaaggga      900

ucgcuaguaa caugcgccaa auucacaugc uccaagaaaa ugacaggcaa aagcauccag      960

cccgaaaacc uagaguaccg aauaaugcug ucaguccacg gcucacaaca cuccggcaug     1020

auaguaaacg acaccggcua cgaaacggau gaaaacagag ccaaagucga gguaaccccg     1080

aacuccccac gagcagaagc aacucucgga ggguucggga gccucggguu agacugcgaa     1140

ccccgcaccg gauuggacuu cagcgaccuc uauuaccuga caaugaacaa caaacacugg     1200

cucguccaca aagagugguu ucacgacauc ccccuccccu ggcacgcagg cgccgacaca     1260

ggaacgccac acuggaacaa caaagaggcc uuggucgagu ucaaagacgc acacgccaaa     1320

agacaaaccg uggucguacu cggcagccaa gaaggagcag uacacacagc gcuagcaggc     1380

gcacuagaag ccgaaaugga cggagcaaaa gggaaauugu ucucuggcca ccucaaaugc     1440

agacugaaaa uggacaaacu aagacugaaa ggagugucau acucacugug cacagcagca     1500

uucaccuuca caaaaguacc agcggaaacc cuucacggga caguaaccgu agaaguccaa     1560

uacgcaggaa ccgacgggcc gugcaagauc ccagugcaga uggccgucga caugcagacc     1620

cuaaccccag ucgggcgacu caucaccgcc aacccaguaa uaacagaaag uaccgaaaac     1680

agcaaaauga ugcuugaacu agacccaccc uucggagaca gcuacauagu aauaggcgug     1740

ggagauaaga aaauaaccca ccacuggcac agaagcggga gcacaauagg aaaagcauuc     1800

gaggccaccg ucaggggagc aaaaagaaug gcaguacucg gagacaccgc cugggacuuc     1860

gggucugucg gaggaguauu caacucacua gggaaaggaa uccaccaaau auucggcgcc     1920

gccuucaaga gcuuauucgg gggcaugucc ugguucagcc aaauccuaau uggaacccuc     1980

cuaguguggc uaggccuaaa caccaaaaac ggaagcauaa gucuaacaug ucuagcccua     2040

ggcggcguga ugauauuccu aagcaccgcg guaagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5124
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 1 opt

<400>  5124
gggagaaagc uuaccaugac aagcaucggc auaaucggac uauuacucac aaccgcaaug       60

gcagccgaaa uaacccgccg aggaucugcc uacuacaugu accucgacag gagcgacgcc      120

ggaaaagcaa uaucauucgc aacaacccua gggguaaaca agugccacgu acaaauaaug      180

gaccucggcc acaugugcga cgccacaaug agcuacgaau gccccaugcu ggacgagggc      240

guggaaccgg acgacgucga cugcuggugc aacaccacuu ccaccugggu cguauacgga      300

accugccacc acaagaaagg ggaagcacga agauccagac gagccgucac auuaccaagc      360

cacucaacca gaaaacuaca gacaagaagc caaacauggc ucgaaagcag ggaguacaca      420

aaacaccuga uaaaagucga aaacuggaua uuccgcaacc ccggguucgc ccucguggcc      480

guagcaauag ccuggcuacu aggcuccagc acaagccaaa aagucaucua ccuagucaug      540

auccuccuaa ucgcgccagc auacuccauc cgguguauag gcgucucaaa cagagacuuc      600

gucgaaggga ugucaggcgg aaccugggua gacgugguac ucgagcacgg aggcugcgua      660

acagucaugg cccaagacaa acccaccguc gacaucgaac ugguaacaac caccguaagc      720

aacauggcug aaguccgauc cuacugcuau gaagccagca ucucagacau ggcaagugac      780

ucacgaugcc caacccaagg cgaagccuac cuggacaagc aaagcgacac ccaguacguc      840

ugcaaacgca cccuagucga ccgagggugg ggaaacggcu gcggccucuu cggaaaagga      900

ucgcuaguca cgugugcaaa guuuaccugc uccaagaaaa ugacaggcaa aaguauacag      960

ccugaaaacc ucgaguaucg aaucaugcua ucugugcacg gcucacaaca cucaggaaug     1020

acggucaacg acauaggaua cgagaccgac gaaaaccgcg caaaagucga ggucacccca     1080

aacagcccac gggcagaagc cacccucgga ggguucggca gccuaggccu cgauugcgaa     1140

ccaagaacag gccuugacuu cuccgaccua uacuaucuca ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacaua ccacuccccu ggcacgcagg cgcggacaca     1260

gggacgccac acuggaacaa caaagaagcc cuaguagagu ucaaagacgc acacgccaaa     1320

cgacaaaccg ucgucguucu aggcucccaa gaaggcgcag uccacacggc ccuagcagga     1380

gcgcuagaag ccgagaugga cggcgccaag ggcaaacucu ucagcggaca ccuaaaaugc     1440

aggcuaaaaa uggacaaacu acggcucaag ggaguaucau acucgcugug caccgcggcg     1500

uucaccuuca ccaaaguacc cgccgagacc cuccacggaa ccguaaccgu cgaaguccaa     1560

uacgcaggga cugacggccc gugcaaaauc ccaguccaaa uggcagucga caugcaaaca     1620

cuaacaccag ucgggcgacu caucacagca aaccccguaa ucacagaauc caccgaaaac     1680

uccaagauga ugcucgagcu agacccgcca uucggagaca gcuacaucgu aauaggaguc     1740

ggagacaaaa agaucaccca ccacuggcac cgcucgggaa gcacaaucgg caaggccuuc     1800

gaagcaacug uaagaggcgc aaaacgcaug gccguacuag gagacaccgc uugggacuuc     1860

ggcagcgucg gcgggguguu caacucccua ggaaaaggca uacaccaaau auucggagca     1920

gcuuuuaaau cccuguucgg cggcaugagc ugguucagcc aaauacuaau aggcacccuc     1980

uuagucuggc uaggacucaa cacaaagaac ggcucaaucu cacuaaccug ccucgcacua     2040

ggaggaguca ugauauuccu gagcacagcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5125
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 1 opt

<400>  5125
gggagaaagc uuaccaugac aagcauaggc auagucggcc ugcuacucac cacugccaug       60

gccgccgaaa uaacacgcag aggcuccgcc uacuacaugu accucgaccg cuccgacgca      120

ggaaaagcca ucuccuucgc aaccacgcuc ggcgucaaca aaugucacgu acaaauaaug      180

gaccuaggcc acaugugcga cgccaccaug agcuacgaau gccccaugcu cgacgagggc      240

gucgagccgg acgacguaga cugcuggugc aacacaaccu cuacaugggu agucuaugga      300

accugccauc acaagaaagg cgaggcccga cgaagccgac gagcaguuac cuuacccagc      360

cacagcacac gcaaacucca aacaagaagc caaacauggc uagagagccg agaauacaca      420

aaacaccuca ucaaagucga aaacuggaua uuccggaauc caggcuucac acucgucgca      480

guagcaauag ccuggcuccu cggcagcagc accagccaaa aaguaaucua ccuagucaug      540

auccuccuaa uagcgccagc cuacuccaua aggugcauag gaguaagcaa ucgcgacuuc      600

guagaaggaa ugagcggagg caccuggguc gacguagucc ucgaacacgg gggaugcgua      660

acaguaaugg cccaagacaa gccaacagua gacauagagc ucgucacaac gaccgucagu      720

aacauggcag aagucagguc uuacugcuac gaagcaagca ucagcgacau ggcaucagac      780

agccgaugcc ccacccaagg agaagcguac cuagacaagc aaucagacac ccaauacguc      840

ugcaaaagga cccuuguaga uagaggaugg ggaaacggau gcggccucuu cggcaagggc      900

agccuaguaa ccugcgcaaa auucacaugc ucaaagaaaa ugaccggcaa aucaauccaa      960

ccagaaaacc uugaauaccg aaucaugcuc uccguccacg gaagccaaca cagcggaaug     1020

auagucaacg acgaaaacag agcaaaaguc gaagucacuc ccaacucacc ccgagccgaa     1080

gccacccugg gcggcuucgg aucccuggga cuugacugcg aaccucgcac cggccuagac     1140

uucagcgacc uauacuaccu cacaaugaac aacaaacacu ggcuagucca caaagaaugg     1200

uuucacgaca uaccccuccc auggcacgcc ggcgcagaca ccggaacacc ccauuggaac     1260

aacaaagaag cccuagucga guucaaagac gcacacgcaa aacggcagac aguagucgua     1320

cuaggcuccc aggagggcgc aguacacacc gcacuggccg gcgcccugga agccgaaaug     1380

gauggcgcaa aaggaaggcu guucucggga caccucaaau gcaggcugaa aauggacaaa     1440

cuaagacuca aagggguauc auacuccuua ugcacagccg ccuucaccuu caccaaagua     1500

ccagcagaga cgcuccacgg caccgugacc gucgagguac aauacgccgg caccgacgga     1560

ccaugcaaag uccccgccca aauggcagua gauaugcaga cacugacccc agucggcaga     1620

cuaauaaccg ccaacccggu uauaaccgaa agcaccgaaa acucaaaaau gauguuggaa     1680

cuggacccac ccuucggcga uagcuacaua gucauaggug uaggagacaa gaaaaucacc     1740

caccacuggc acagaucagg uagcacaauc ggcaaagcgu ucgaagccac cguccgcggc     1800

gcaaaaagga uggccguacu cggcgacacc gccugggacu ucggcagcgu gggaggaguc     1860

uucaacagcc uaggaaaagg cauccaccaa aucuucggag ccgccuucaa aagccucuuc     1920

ggaggaauga gcugguucuc ccaaauccuc aucggaaccc uacuggucug gcucggccuc     1980

aacaccaaaa acggcagcau cucucuaacc ugccucgcac uaggcggcgu caugauauuc     2040

cucagcaccg cggucucagc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  5126
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 1 opt

<400>  5126
gggagaaagc uuaccaugac cucaaucgga aucaucggac uauugcucac caccgcgaug       60

gcagcagaaa ucacacgcag aggauccgca uauuacaugu accuugaccg cagugacgcc      120

ggcaaagcaa ucucuuucgc cacaacacuc ggagucaaca aaugccacgu ccaaaucaug      180

gaccucggcc acaugugcga cgccacaaug uccuacgaau gccccaugcu cgacgaaggc      240

gucgaacccg acgacgucga cugcuggugc aacaccacau ccacuugggu cgucuacgga      300

accugccacc acaagaaagg cgaggcgaga cgcuccagac gcgccgucac acuacccucg      360

cacuccacaa ggaaacucca gacccgaagc cagaccuggc uagagucccg ggaauacacc      420

aaacacuuaa ucaaaguaga gaacuggaua uuuagaaacc ccggauucgc ucucgucgcc      480

gucgcaauug cuuggcuccu aggcucaagc accagucaaa aaguuauuua ccuagucaug      540

auacuacuaa ucgcacccgc cuacuccauc cgcugcaucg gagucaguaa ccgcgacuuc      600

guggaaggca uguccggagg aacguggguc gacguagucc ucgagcacgg aggaugcguc      660

acagucaugg cacaggacaa accaaccguc gacauagagc uagucaccac gacagucuca      720

aacauggccg aaguaagguc uuacugcuac gaagccagca ucuccgauau ggccucagac      780

ucccgaugcc ccacccaagg agaggccuac cucgacaaac aaucagacac ccaauacgua      840

ugcaaaagaa cccuaguuga ccgcggcugg ggaaacggcu gcggccucuu cggcaaaggg      900

agccuaguaa ccugcgcaaa auucacaugc uccaagaaaa ugaccggcaa auccauccaa      960

ccagaaaacc ucgaguaccg cauaaugcuc ucaguacacg gcucacaaca cagcggaaug     1020

auaggcuacg aaaccgacga aaaccgcgcc aaagucgaag ucacccccaa cagcccacgc     1080

gccgaagcca cacucggcgg auucggcucc cuaggccuag acugcgaacc ccgcaccggu     1140

cuugauuuuu cagaccuuua uuaccucaca augaacaaca aacacuggcu cgugcauaaa     1200

gaaugguuuc acgauauacc acuaccgugg caugcgggag cagacaccgg cacgccccac     1260

uggaacaaca aagaagcacu cgucgaguuc aaagacgccc augccaaacg ccaaaccgua     1320

guaguucuag gaucacaaga aggagcaguc cacaccgccc ucgccggagc cuuagaagca     1380

gaaauggacg gagcgaaagg caaacuguuc agcggccacc uaaagugcag acucaaaaug     1440

gacaagcucc gccucaaagg cgucucauac ucacuaugca ccgcagccuu caccuucacc     1500

aaggucccag cagaaacccu ccacgggacc gucacugucg aaguccagua ugcaggcacg     1560

gacggaccau gcaagauccc gguccagaug gcaguagaca ugcaaacccu cacacccguc     1620

ggccgacuca ucacagcaaa ccccgugauc acagaaucca cugaaaacuc aaaaaugaug     1680

cucgaacucg acccgcccuu cggcgacagc uacaucguca ucggcgucgg cgacaaaaag     1740

auaacccacc acuggcaccg cagcggguca acuaucggca aagcauucga agccaccguc     1800

cgcggcgcca aacgaauggc cguacucgga gacacggccu gggauuucgg cuccguaggc     1860

ggaguauuca acagccuagg aaagggcauc caccaaaucu ucggagcagc auucaaaagc     1920

cuauucggcg gcaugucaug guuuucccaa auacugaucg gcacacucuu agucuggcuc     1980

ggacucaaca ccaagaacgg cuccaucagc cuaaccugcc ucgcacuagg cggagucaug     2040

aucuuccuaa gcaccgccgu aagcgcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  5127
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 1 opt

<400>  5127
gggagaaagc uuaccaugac cuccgucgga auagucggcc ucuuacuaac cacugccaug       60

gccgcagaag uaacccgccg agggagcgca uacuacaugu accucgaccg caacgacgca      120

ggagaagcca ucagcuuccc cacgacgcua ggcaugaaca agugcuacau acagaucaug      180

gaccuaggcc auaugugcga cgccacaaug ucauacgaau gcccaaugcu agacgaaggc      240

gucgaacccg acgacgucga cugcuggugc aauacaacaa gcacuugggu agucuacgga      300

accugccacc acaagaaagg ggaagcacga cgaagccgcc gagcagucac ucuccccuca      360

cacagcaccc ggaaacucca aacacgcagc caaaccuggc uagaaagccg agaauauaca      420

aaacaccuca uacgaguaga aaacuggauc uuccgaaacc caggcuucgc auuagccgcc      480

gcggccaucg ccuggcuacu gggcucguca acaucacaga aaguuaucua ccuaguaaug      540

auacuucuaa ucgcccccgc cuacagcaua agaugcaucg gcguaagcaa cagagacuuc      600

guagagggga ugagcggcgg gaccugggua gacgucgucc uggagcacgg cgguugcguc      660

acgguaaugg cccaagacaa accaaccguu gacaucgaac ucguaacgac gaccgugucc      720

aacauggcag aaguccgauc auacugcuac gaagccagca ucagcgacau ggcauccgac      780

ucuagaugcc cgacccaggg agaagccuac cuugacaaac aaagugacac acaauacguc      840

ugcaaacgaa cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cgggaaaggc      900

agccuaguaa ccugugcgaa auucgcaugc agcaagaaaa ugacagggaa auccauacag      960

ccagaaaacc uagaauaccg cauaaugcug aguguccacg gaucucaaca cagcggcaug     1020

aucguaaacg acacaggaca cgaaacagac gaaaaccgag caaaaguaga gaucaccccg     1080

aacagccccc gagcggaagc aacacuaggc ggauucggca gccugggccu cgacugugaa     1140

cccagaacag gcuuagacuu cagcgaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cucguccaua aagaaugguu ccacgacauc ccgcucccau ggcacgccgg cgcagacaca     1260

ggaacgccac acuggaacaa caaagaggcg cuaguggagu ucaaagacgc acacgccaaa     1320

cgccagaccg uagucguacu aggaucacaa gaaggcgccg uucacacagc ccuagccgga     1380

gcacucgaag ccgaaaugga cggcgcaaag ggcagacuau caucaggcca ccuaaaaugc     1440

cgccuaaaga uggacaaacu gcgccuaaaa ggcgugucau acucacuaug cacagcagca     1500

uucacguuua ccaagauacc agcagaaaca cuacacggua cagucaccgu cgaaguccaa     1560

uacgcaggga ccgacggacc augcaaagua cccgcccaaa uggcagugga caugcaaaca     1620

cucacaccgg uaggacgacu uaucaccgcc aacccgguaa uaacagaauc caccgaaaac     1680

agcaaaauga ugcucgaacu cgacccaccc uucggagacu ccuacauagu aauaggcguc     1740

ggagaaaaga aaaucacaca ccacuggcac cgcuccggcu ccaccaucgg gaaagcauuc     1800

gaagccacag ucagaggagc caaacgaaug gcgguccugg gcgacacggc augggacuuc     1860

ggaagcgucg ggggcgcacu caacucacuc ggccguggaa uacaccaaau cuucggggcc     1920

gcguucaaaa gccucuucgg cggcaugagc ugguucucgc aaauccucau aggcacccua     1980

cucauguggc ucggacuaaa caccaaaaac ggaucaauca gccucaugug cuuagcccua     2040

ggcggcgucc ucauuuuucu cagcaccgcg guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5128
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 1 opt

<400>  5128
gggagaaagc uuaccaugac cuccguagga aucguuggcu uacuccucac caccgcaaug       60

gcagcagaag uaacucggcg cggcucagcc uacuacaugu accucgaccg caacgacgca      120

ggagaagcca uaagcuuccc caccacccuu ggcaugaaca aaugcuacau ccaaauaaug      180

gaccuugguc acaugugcga cgcgaccaug agcuacgaau gccccaugcu agacgaagga      240

gucgaaccag acgacguaga cugcuggugc aacaccacaa gcaccugggu cguauacggc      300

accugccacc acaaaaaagg cgaagccaga cgcucacgac gagccgucac ucuucccucc      360

cacuccaccc gcaaacuaca aacgcgaucc caaaccuggu uagaaagccg cgaauacacc      420

aaacaccuua uaagggucga gaacuggaua uuccgcaauc ccggacuagc ucuugcagcc      480

gcagcaauag ccuggcuacu aggaucgucc accucacaaa aagucauaua ccuagucaug      540

auccuccuaa uagcuccagc cuacagcaua cguugcaucg gcguuuccaa ccgggacuuc      600

guagaaggca ugagcggcgg caccuggguc gacguuguac uggaacacgg aggaugcguc      660

accgucaugg cccaagacaa acccacuguc gacaucgaac uaguaaccac aaccgucagc      720

aacauggccg agguacgcag cuacugcuac gaggcgucca ucagcgacau ggcguccgac      780

ucacggugcc ccacccaagg cgaagcauac cuagacaaac aaucugacac ccaauacgua      840

ugcaaacgaa cccucgugga ccgaggaugg ggcaacggcu gcggccuguu cggcaaaggc      900

ucacuaguca ccugugcaaa auucgccugc uccaaaaaaa ugacagggaa auccauacaa      960

cccgaaaacc uagaauauag aaucaugcua agcgugcacg guucccagca uuccggcaug     1020

auaguaaacg acaccggcca cgaaaccgac gaaaaccgug caaaagucga aauaacucca     1080

aacucacccc gcgccgaagc aaccuuaggc ggcuucggcu cacucggccu agacugcgag     1140

ccccgcaccg gguuagacuu cuccgaccug uacuaccuua ccaugaacaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacaua ccccuaccau ggcacgcggg ggccgacaca     1260

ggaacccccc acuggaacaa uaaagaagcc cucgucgaau ucaaagacgc ccacgcuaag     1320

cgccaaaccg uagucguccu agggucacag gagggagccg uccacacugc ccuagccggc     1380

gcucucgaag cagaaaugga uggcgcaaaa gggcgacuua gcuccggaca ccuaaaaugu     1440

cgccucaaaa uggacaaauu gcgacuuaaa ggagucucuu acucucucug caccgccgcc     1500

uucacauuca caaaaauccc agccgaaacc cuccacggca ccgucaccgu agaaguccaa     1560

uaugcaggca ccgacggacc cugcaaaguc ccugcacaaa uggccgucga caugcaaaca     1620

cucacaccag uaggccgacu cauaacugcc aacccgguaa ucaccgaauc cacugaaaac     1680

ucgaaaauga ugcucgaacu cgacccacca uucggagacu cauacauagu cauagguguc     1740

ggagaaaaaa aaauaaccca ucacuggcac agaucaggcu caaccaucgg caaggcauuc     1800

gaagccaccg uaaggggcgc aaaacgcaug gccguccuag gagacaccgc augggacuuc     1860

ggaucgguag gcggcgcacu caacagccua ggcaaaggca uacaccagau auucggcgca     1920

gccuuuaaaa gccucuucgg cggaauguca ugguucagcc agauccucau aggcacacuc     1980

cuuauguggc uaggccuaaa cacaaaaaac ggauccaucu cccucaugug ccucgcccuc     2040

ggaggaguac ugaucuuccu aucgaccgca gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5129
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 1 opt

<400>  5129
gggagaaagc uuaccaugac aagcguaggu auagucggcc uccuccucac caccgcaaug       60

gcagccgagg ucacacgcag aggcagcgca uacuacaugu accuagaccg gaacgacgca      120

ggagaagcaa ucucguuccc aaccacccuu gggaugaaca aaugcuacau acagauaaug      180

gaccugggac auaugugcga cgccacaaug uccuacgaau gcccaaugcu cgacgagggg      240

guagaacccg augacguaga cugcuggugc aacaccacaa gcacaugggu cgucuacggc      300

acaugccacc acaagaaagg ggaagcccgg aggagcagac gagcagucac acuccccagc      360

cacagcacaa gaaaacuaca gacccgcuca caaaccuggc uagaaagccg agaguacaca      420

aaacaccuca uacgaguuga aaacuggauc uuccgcaacc ccggauucgc gcuagccgcg      480

gcagcaauag cauggcuccu gggaucuagc accagucaaa aaguuaucua ccuugucaug      540

auacuccuaa uagcccccgc auacagcaua cgaugcauag gcguaagcaa ccgcgacuuc      600

gucgaaggga ugucaggagg gaccuggguc gacguaguac uagaacacgg cggaugcgug      660

acagucaugg cccaagacaa acccacagua gacauagagc ucgucacaac cacaguaucc      720

aacauggccg aaguccgcuc guacugcuac gaagcaucaa ucagcgacau ggccucagac      780

ucccgcugcc ccacgcaggg agaagcauac cucgacaaac aaucagacac acaguacguc      840

ugcaaaagga cccuaguaga ccgaggaugg gggaacgggu gcggacucuu uggaaaggga      900

ucgcucguaa caugcgcaaa auucgccugc agcaaaaaga ugacaggcaa aucaauacag      960

ccggaaaacc ucgaauaccg gaucaugcuc uccguacacg gcucacaaca cucgggcaug     1020

auagucaacg acacaggcca cgaaacagac gaaaacaggg ccaaagucga aaucaccccg     1080

aacucaccgc gcgcagaggc uacccuagga ggguucggau cccucggccu agacugcgaa     1140

ccgcgaacag gacuggacuu cagugaccuc uacuaccuaa ccaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacaua ccccuccccu ggcacgcagg agccgauacc     1260

ggcacgccgc acuggaacaa caaagaggcc cuagucgagu ucaaagacgc acacgcaaaa     1320

aggcaaacgg ucgucguacu cggcucgcaa gaaggagcag uacauacagc acuagcaggc     1380

gcacucgagg ccgaaaugga cggagccaaa gggcgccuuu ccagcggcca ccucaaaugc     1440

cggcuuaaaa uggacaaacu uaggcuaaaa ggggucuccu acagccucug caccgccgcc     1500

uucacauuca cuaaaauccc agcagagacc cuacacggaa cagugaccgu agaagugcaa     1560

uacgccggga cagacggacc cugcaaagua cccgcacaaa uggccguuga caugcagaca     1620

cuaacccccg ucggacgacu aaucaccgcc aauccgguga uaaccgaauc gacugaaaac     1680

agcaaaauga ugcucgaacu cgacccaccc uucggcgaca gcuacaucgu aauaggaguc     1740

ggcgaaaaga aaauaaccca ccacugguac cgcuccggaa gcaccaucgg caaagcguuc     1800

gaagccacgg ucagaggugc aaaacgcaug gccguccuag gcgauacggc cugggacuuc     1860

ggaagcguug ggggagcacu aaacucacua ggcaaaggga uacaccaaau auucggugcc     1920

gcauucaaau cccuguucgg aggaauguca ugguucucac aaauacuaau aggcacacua     1980

cuaauguggc ugggccucaa caccaagaac ggaagcauuu cccuaaugug ccucgcccuc     2040

ggcggcguac uaaucuuccu auccacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5130
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 1 opt

<400>  5130
gggagaaagc uuaccaugac gucuguaggc auaguuggcc uacuccucac aacagccaug       60

gcagccgaag uaacccgccg cggcucagcc uauuacaugu accuggaccg aaacgacgcu      120

ggagaagcca ucucauuccc caccacccuc ggaaugaaca aaugcuacau acaaaucaug      180

gaccucggac acaugugcga cgcgaccaug aguuacgaau gcccaauguu ggacgaagga      240

gucgagcccg acgacguuga cugcuggugu aacaccacca guacuugggu cgucuacgga      300

accugccacc acaagaaagg ggaagcccga cgaucccgcc gggcgguaac ccuaccuucc      360

cacagcaccc gcaaacucca aacacgauca caaaccuggc ucgaaagcag agaauacacg      420

aaacaucuca uacgaguaga gaacuggaua uucagaaauc cgggauucgc acuagccgcc      480

gccgccaucg ccuggcuccu gggaucauca accagccaaa aaguaauaua ccucgugaug      540

auccuccuaa uagcccccgc guacucaaua cgcugcauag gcgucagcaa ccgggacuuc      600

guagaaggca uguccggggg caccuggguc gacguaguac ucgagcacgg cggcugcgug      660

accguaaugg cccaagacaa acccaccgua gacaucgaac ugguuacaac cacaguguca      720

aacauggcag aagucagaag cuacugcuac gaagccucga uaagcgacau ggccuccgac      780

agccgaugcc ccacccaagg agaagccuac cuagacaaac aaucagacac acaauacguc      840

ugcaaacgaa cccuaguaga ccgcggcugg ggcaacggau gcggccuuuu cggaaaaggc      900

ucacuuguaa ccugcgccaa auucgcaugc agcaagaaaa ugaccggaaa aucaauccaa      960

ccagaaaacc uagaguaucg caucaugcuc uccguacacg gaucacaaca cucaggcaug     1020

auagucaaug auacgggcca cgaaaccgac gaaaauagag caaaagucga gaucaccccc     1080

aacagccccc gcgccgaagc aacacucgga ggauucggga gccucggacu cgacugcgaa     1140

ccccgaacag gccuagacuu cucagaccua uacuaccuca ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ucacgacaua ccacuacccu ggcacgcugg ggccgacacc     1260

gggacucccc acuggaauaa caaagaagcc cuaguagagu ucaaagacgc ccacgccaaa     1320

cgccaaacgg ucguaguacu cggcagccaa gaaggggccg uccacaccgc gcucgccgga     1380

gcccucgaag ccgaaaugga cggagcaaag ggacggcuau ccucagggca ccucaaaugc     1440

agacuaaaga uggacaaacu ccgucugaag ggcguuuccu auagccucug caccgcagca     1500

uucaccuuca caaagauccc ggccgaaaca cuccacggga ccgucaccgu agaagugcaa     1560

uacgccggca cagauggccc cugcaaaguc cccgcccaga uggccgucga cacccaaacg     1620

cugacacccg ucggccgccu aaucaccgcg aaucccguca uaacugaauc caccgaaaac     1680

ucgaaaauga uguuagaacu cgauccccca uucggggacu cauacaucgu aauaggcguc     1740

ggagaaaaga aaauaaccca ccacuggcac cgaucaggca gcaccauagg caaagccuuc     1800

gaagccacug uaaggggcgc aaaacgcaug gcaguacuag gagacaccgc augggacuuu     1860

ggcucagucg gcggagcccu caacucccua ggcaaaggaa uacaccagau uuucggagca     1920

gcuuucaaaa gccucuucgg cggcaugagc ugguucuccc agauucuaau aggcacccug     1980

cucauguggc ucggacugaa caccaaaaac ggauccaucu cccucaugug ccuagcacug     2040

ggcggcgucc uaauauuccu gucuaccgcc guauccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5131
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 1 opt

<400>  5131
gggagaaagc uuaccaugac auccgugggc auagucgguc uccuacuuac cacagcaaug       60

gcagccgaag uaacacgccg aggcucagca uacuacaugu accucgaccg gaacgacgca      120

ggcgaagcaa uauccuuccc aaccacacuc ggcaugaaca aaugcuacau ccaaaucaug      180

gaccucggcc acaugugcga cgccacaaug uccuacgaau gccccaugcu cgacgaaggg      240

guggaaccag acgacgucga cuguuggugc aacaccaccu ccaccugggu cgucuacggg      300

accugccacc acaagaaagg cgaagcccgc cgaagcagac gcgcagucac acucccuucc      360

cacucaaccc gcaaacugca aacccgcuca caaaccuggc ucgaaagccg cgaauacacc      420

aaacaccuua uaagaguaga aaacuggauc uuccgaaacc caggcuucgc acucgccgca      480

gccgccauag ccuggcuacu ugggagcucc acgucccaaa aaguaauaua ccucguaaug      540

auucuccuaa uagcgccagc cuacuccauc cggugcauag gagucucaaa cagagacuuc      600

gucgaaggua ugucgggagg aaccuggguc gacguaguac uagaacacgg gggcugcguc      660

acugucaugg cacaagacaa accgaccguc gacauagaac ucguaacaac caccgugucu      720

aacauggccg aaauccgaag cuacugcuac gaagcaagca uaagcgacau ggccuccgac      780

ucgcgaugcc caacccaagg agaagccuac cuugacaaac aaagugauac acaauacguc      840

ugcaagagaa cauuaguaga ccgaggaugg gggaacggau gcggccuauu cggcaaaggc      900

ucacucguca cgugcgccaa guucgcuugc uccaagaaaa ugaccggcaa aagcauacaa      960

ccugaaaacc ucgaauacag aauaaugcua uccguccacg gcucccaaca cagcggaaug     1020

aucgucaacg acacaggaca cgaaacagac gaaaaccgcg ccaaaguaga aaucaccccc     1080

aacucacccc gagcagaagc cacucucggc ggcuucggcu cccuaggacu agacugcgaa     1140

ccccgaaccg gccuggacuu uuccgaccuc uacuaccuga ccaugaauaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacaua cccuuacccu ggcacgccgg cgccgacacc     1260

ggcacacccc acuggaacaa caaagaagcc cuggucgagu ucaaggacgc acacgcaaaa     1320

cgucaaaccg uaguaguccu cggcucccaa gaaggcgccg uccacaccgc ccuagcaggg     1380

gcacuagaag cagaaaugga cggggccaaa ggaagacuau ccagcggcca ccucaaaugc     1440

cgacucaaaa uggacaaacu cagacucaaa ggaguaucau acagccuaug uaccgcagcg     1500

uucaccuuca ccaaaauccc agccgaaaca cuacacggga caguaaccgu agaaguacaa     1560

uacgccggaa cugacggacc cugcaaagua cccgcacaaa uggcugugga caugcaaacc     1620

uuaaccccag ugggcagacu gaucacagcc aaccccguaa uaacggaauc gacggaaaac     1680

uccaaaauga ugcucgaacu agacccuccc uucggcgacu cauacaucgu caucggcguc     1740

ggcgagaaaa agaucaccca ccauuggcac cgaagcggca gcacgauagg caaagcauuc     1800

gaagcaacag uaagaggcgc caaacgaaug gccguccuag gagacaccgc augggacuuc     1860

ggcagcguag gaggcgcacu aaacucacuc ggaaaaggaa uccaccaaau cuucggagca     1920

gccuucaaau cccuauucgg gggcaugagu ugguucuccc aaauacucau cggcacccua     1980

cuuauguggc ucggccuuaa caccaagaac ggaagcauaa gccugaugug ccucgcccug     2040

ggcggcgucc ucaucuuccu uucuaccgca guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5132
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 1 opt

<400>  5132
gggagaaagc uuaccaugac cagcguggga aucgucggac uacuccuaac aaccgcgaug       60

gcugccgaag uaacccgacg cgggagcgcc uacuacaugu acuuagacag aaacgacgca      120

ggggaagcaa ucagcuuccc uacaacgcuc ggaaugaaca aaugcuacau acaaauaaug      180

gaccucggac acaugugcga cgcaacaaug aguuacgaau gcccaaugcu agacgaaggc      240

guagaaccag acgacguaga cugcuggugc aacaccacga gcacgugggu agucuacggu      300

acgugucacc acaagaaagg cgaagcacga cgcuccagga gagccguaac gcucccgagc      360

cacucgacac gaaaacucca aacacgcagc caaaccuggc uagaaucacg agaauacacg      420

aagcaccuaa uccgaguaga aaacuggaua uuccgaaacc ccggauucgc gcucgccgcc      480

gcggccaucg cauggcuccu ugggagcagc acaagccaaa aagugauaua ccuaguaaug      540

auccuacuca ucgccccagc auacaguaua cgaugcaucg gagugagcaa ccgcgacuuc      600

guggaaggaa uguccggggg caccuggguc gacgucgucc uggaacacgg cggaugcguc      660

acggucaugg cacaagacaa accaacagug gacauagaac ucgucaccac cacagucagc      720

aacauggcag aaguccgcag cuacugcuac gaagccagca ucucagacau ggccuccgac      780

agcagaugcc ccacccaagg cgaagcguac cuagacaaac aguccgacac gcaauacguc      840

ugcaaacgga cacucguaga cagaggaugg ggaaacggcu gcggccuguu cggcaaaggc      900

ucccuaguca ccugugcaaa guucgccugc agcaagaaaa ugacaggcaa aucaauccag      960

ccggaaaacc ucgaauacag aauaaugcuc agcguccacg gcucccaaca uagcggcaug     1020

auaguaaacg acacgggcca cgaaacagac gaaaaccgag ccaaagucga aauaacaccc     1080

aacagccccc gcgcggaagc cacacucgga ggcuucgggu cgcuaggacu cgacugugaa     1140

ccaagaacag gccuggacuu cagcgaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cuugugcaua aagaaugguu ccacgacaua ccacucccgu ggcaugccgg agcagacacc     1260

ggaacaccac acuggaacaa caaagaagcc cuaguggagu ucaaagacgc acacgcaaaa     1320

cgacaaacag uagucguacu aggaucccaa gaaggcgcug uacacaccgc acuagccggc     1380

gcacuagaag ccgaaaugga cggagcaaaa ggacgccuau ccucaggcca ccucaagugc     1440

aggcuaaaga uggacaaacu ccgauuaaag ggagucuccu acagccugug caccgccgcc     1500

uucaccuuca ccaaaauccc agccgaaaca cugcacggga ccgugacagu agaaguacaa     1560

uacgccggca cagauggacc cugcaaagua cuagcacaaa uggccgucga caugcaaaca     1620

cuaaccccag ucggccgccu cauaacggca aaccccguga ucacagaauc cacagaaaac     1680

ucgaaaauga ugcuagaacu cgacccaccc uucggcgacu cauacauagu aaucggagua     1740

ggagaaaaga aaauaaccca ccacuggcac agaagcggaa gcacaauagg caaagccuuc     1800

gaagcaaccg uccgaggagc caaaagaaug gcagucuuag gcgacaccgc augggacuuc     1860

gggagcgugg gcggagcacu aaacagcuug ggaaaaggaa uccaccaaau auucggagca     1920

gcguucaaaa gccuguucgg aggcaugagu ugguucaguc agauccucau cggcacacuc     1980

cucauguggc uaggacuaaa caccaaaaac ggcagcaucu cacugaugug ccucgcgcuc     2040

ggaggcguac ugauauuccu gagcaccgcc guauccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5133
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 1 opt

<400>  5133
gggagaaagc uuaccaugac cucagucggg auugucggac uccuacucac cacagccaug       60

gcagccgagg ugacccgacg aggaucagcc uacuacaugu aucucgaucg caacgacgca      120

ggcgaagcca uaucguuccc cacaacccuc ggcaugaaca aaugcuacau ccaaaucaug      180

gacuuaggcc auaugugcga cgccaccaug ucauacgaau gccccacacu agacgaaggc      240

gucgaacccg acgacgucga cugcuggugc aauaccaccu ccacaugggu cguauacggc      300

acaugccacc acaagaaagg cgaagcccgc cgaucccgca gagccguuac cuuaccaagc      360

cacuccaccc gaaaacucca aacgcggagc caaaccuggc uagaaagccg cgaauacaca      420

aaacaccuca uccgcgucga aaacuggaua uuccgcaacc caggauucgc ccucgccgcc      480

gccgcaauag cauggcugcu cggcuccagc acaucccaaa aagucaucua ccucguaaug      540

auccuucuca ucgcccccgc cuacuccauc cgcugcaucg gcgucucaaa ccgagacuuc      600

gucgaaggca ugucaggcgg caccuggguc gacgucgucc uggaacacgg aggcugcgua      660

accgucaugg cccaagacaa accgacagua gacaucgagc uagugaccac aacagucagc      720

aauauggccg aagucagauc uuacugcuau gaagcgucca ucucugacau ggccuccgac      780

ucacgcuguc ccacgcaagg ggaagccuac cuggacaaac aaagcgacac gcaguacguc      840

uguaaacgca cccuagucga ccgcggcugg ggcaacggau gcggacuauu uggaaaaggc      900

ucacucguca cuugcgccaa auucgccugc ucaaagaaga ugaccggaaa gucuauccaa      960

ccggaaaacc uagaauaccg cauaaugcua uccguccacg ggucacaaca cucaggaaug     1020

auagucaaug acaccggaca cgaaaccgac gaaaaccgug cuaaaguaga aaucaccccc     1080

aacuccccua gagccgaagc aacccuaggg ggauucggaa gucuaggccu ggacugcgaa     1140

ccacgcaccg gccuggacuu cuccgaccuc uacuaccuca caaugaacaa caagcacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccacuaccuu ggcacgccgg cgccgacaca     1260

ggcacacccc acuggaacaa caaagaagcc cucguagagu ucaaagacgc ucacgccaaa     1320

cgucaaaccg ucgucguccu agggagccaa gaaggcgcag uccacaccgc guuggcaggc     1380

gcccuagaag cagaaaugga cggagcaaaa ggacgacucu ccucgggcca ccuaaaaugc     1440

cggcuaaaaa uggacaaacu acgccucaaa ggcgucuccu acagccucug caccgcagcc     1500

uucaccuuca ccaagauccc agccgaaacc cuucacggca ccguaaccgu agaaguccaa     1560

uacgcaggca cagacggccc augcaaagua ccagcccaaa uggcaguaga uaugcaaaca     1620

cuaaccccug uuggccgccu caucacagcc aaccccguaa ucaccgaaag caccgagaac     1680

ucaaaaauga ugcuagagcu cgacccaccc uuuggcgauu ccuacaucgu caucggggua     1740

ggcgaaaaga aaaucacgca ccacuggcac agguccggaa gcacaaucgg caaagcguuc     1800

gaggcaacug uccgaggcgc caaacgaaug gcaguccucg gugacaccgc cugggauuuc     1860

ggaagcgucg gcggugcccu caauagccua ggcaagggca uccaccaaau cuucggcgcc     1920

gccuucaaau cccuguucgg aggcauguca ugguucucac agauccuaau aggcacccug     1980

cuaauguggc uaggccucaa caccaaaaac ggaucgaucu ccuuaaugug ccuagcacuc     2040

ggcggggucc uaaucuuccu auccacagcc guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5134
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 1 opt

<400>  5134
gggagaaagc uuaccaugac cucgguaggg aucgucggcc uacuccuaac aacagccaug       60

gccgccgaag ucacccgccg aggaagugcc uacuacaugu acuuagaccg aaacgacgcc      120

ggggaggcca uaucguuccc cacaacacua ggaaugaaca aaugcuacau ucagauaaug      180

gaccuaggac acauguguga cgcaaccaug aguuacgaau gucccaugcu ggacgaagga      240

guagagcccg acgacguaga cugcuggugc aacacaacca gcacuugggu agucuacgga      300

acgugucauc acaagaaagg cgaagcacga cgaucccgaa gggcuguaac gcugccauca      360

cacucgaccc gaaaacuuca aacacgaucg caaacauggc uagagucccg cgaauacaca      420

aaacaccuca uacgcgucga aaacuggaua uuccgcaacc ccggcuucgc ccuggccgca      480

gccgccaucg cauggcuccu cggcagcagu accucccaaa aagucaucua ccucguaaug      540

auccuacuaa ucgccccagc auacagcaua cgaugcaucg gaguaucaaa ccgcgacuuc      600

gucgaaggca ugagcggcgg aacaugggua gacguaguau uagaacacgg aggcugcgua      660

acuguaaugg cgcaagacaa accgacagua gacaucgagc uagucaccac caccgucagu      720

aacauggcag aaguccgguc auacugcuac gaagccagca uaucagacau ggcguccgac      780

agccggugcc ccacccaggg cgaagccuac cuagacaaac aauccgacac ccaauacgug      840

ugcaaaagaa cccuaguaga cagaggaugg ggcaacggau gcggccuauu cgggaaaggg      900

ucgcucguaa caugcgcaaa auucgccugc ucaaagaaaa ugacagggaa aucaauccaa      960

ccagaaaacc uagaauacag aaucaugcua ucuguccacg gcucccagca cagcggcaug     1020

auagucaacg acacaggcca cgaaaccgac gaaaacaggg ccaaaguaga aaucacacca     1080

aacuccccac gcgccgaagc aacauuaggc ggcuucggca gccuaggccu cgacugcgag     1140

ccccgaacgg gccuugacuu cuccgaccua uacuaccuca ccaugaacaa caaacacugg     1200

cuuguacaca aagagugguu ccacgacaua ccccuacccu ggcacgcagg cgccgacaca     1260

ggcacacccc acuggaacaa caaagaagcc cuggucgagu ucaaagacgc acacgcaaaa     1320

cgccaaacag uaguuguacu cggaagccag gaaggcgcug uacacacagc ccucgcagga     1380

gcucuggaag ccgaaaugga cggugcaaaa ggccgacucu caagcggcca ccugaaaugc     1440

agacuaaaaa uggacaaacu cagacucaaa ggggugagcu acagccucug cacagccgca     1500

uucacauuca caaaaauacc agccgaaacc cuucacggua cagucacagu ggagguccaa     1560

uacgcaggaa cggacggacc augcaaagug cccgcccaaa uggcagucga caugcaaacc     1620

cucacgcccg uagggaggcu cauaacagcu aaccccguaa uaacagagag cacggaaaac     1680

agcaaaauga ugcucgagcu cgacccgccc uuuggggaca gcuacauagu caucggcgua     1740

ggagaaaaga aaaucacaca ucacuggcac cgaagcggcu caacaauagg caaagcguuc     1800

gaagcaacag uacgaggcgc caaaagaaug gcugugcucg gagacaccgc cugggacuuc     1860

gguuccguag gcggagcacu caacucucug ggcaaaggca uacaccaaau cuucggagca     1920

gccuucaaau cccucuucgg aggaauguca ugguucagcc aaauccucau aggaacacug     1980

cucauguggc ucggacuaaa caccaaaaac ggcagcauau cccuaaugug ccuggcacua     2040

ggaggcgucc uaauauuccu cucuaccgug guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5135
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 1 opt

<400>  5135
gggagaaagc uuaccaugac cagcguaggc aucgucgggc uacuacuaac aaccgcgaug       60

gccgccgaag ucacacgcag aggguccgcc uauuacaugu accuagacag gaacgacgcc      120

ggcgaagcaa uaucauuccc gaccacgcug gggaugaaca aaugcuacau acagauaaug      180

gaccucgggc acaugugcga cgcaacaaug aguuacgaau gccccaugcu ggacgaaggc      240

guagaaccgg acgacgucga cugcuggugc aacacaacga gugcaugggu ggucuacgga      300

accugccacc acaagaaagg cgaagcgcgc agaagccgcc gagcagucac acuaccaagc      360

cacaguacac guaagcucca aacaagacca caaacauggc ucgagucccg agaguacaca      420

aaacacuuaa uacgaguaga gaacuggauc uucagaaacc caggauucgc acucgcagcc      480

gcagcaaucg cauggcuacu ggguuccagc accagccaaa aagucaucua ccucguaaug      540

auacuccuaa ucgccccggc cuacuccauc aggugcaucg gaguaagcaa ccgcgauuuc      600

gucgaaggga ugucgggagg aaccugggua gacgucguac ucgaacacgg cggcuguguc      660

acgguaaugg cccaagacaa accaacugua gacaucgagc uaguaaccac gaccguauca      720

aacauggccg aagucagaag cuacugcuac gaagcaucaa ucagcgacau ggccuccgac      780

agcagaugcc ccacccaagg cgaagccuac cuggacaaac aaagcgacac acaauacgua      840

ugcaagagaa cacuggucga ccgaggaugg ggcaacggcu gcggccucuu cgguaaaggg      900

ucgcuaguua caugcgccaa auucgcgugc agcaagaaaa ugaccggaaa aagcauccaa      960

ccagaaaacc ucgaauacag aaucaugcua uccguacacg gcagccagca cagcggcaug     1020

auaguaaacg acaccgggca cgaaacagac gaaaacaggg caaaagucga gauaacaccc     1080

aacucuccac gagccgaagc aacccucgga ggauucggga gccucggacu agacugugaa     1140

ccgaggaccg gccuagacuu cagcgaccug uacuacuuaa caaugaauaa caaacacugg     1200

cuuguacaca aagagugguu ccacgacauc ccccuaccau ggcacgccgg cgcagacacc     1260

ggaacgccgc acuggaacaa uaaagaggcc cuaguagagu ucaaagacgc ccacgcaaaa     1320

cgacaaacug uagucguccu aggcucacaa gagggagcag uccauaccgc ccuagccgga     1380

gcgcucgaag cagagaugga cggagcgaaa gggcgccuau caagcggaca ccuaaagugc     1440

aggcuaaaaa uggauaaacu gcgccucaag ggcgucagcu acucacucug cacagcagca     1500

uucacauuca caaaaauccc ggccgaaacc cuacacggaa ccguaacagu agaaguacag     1560

uacgcaggaa ccgacggacc cugcaaaguc ccggcccaaa uggcgguuga caugcaaacc     1620

cuaacgcccg uaggacgccu gaucacagcc aaccccguaa ucacagaauc aaccgaaaau     1680

ucaaagauga ugcuggaacu ggacccgcca uucggagaca gcuacaucgu aauagggguc     1740

ggcgaaaaga aaauaacaca ccacuggcac cgauccggaa gcaccauugg aaaagcauuc     1800

gaggccacag ucaggggagc gaaacgaaug gcaguccucg gggauacagc augggacuuc     1860

ggauccguag gcggagcccu gaacucacug ggcaaaggaa uccaccaaau auucggagca     1920

gcguucaaau cacuauucgg aggcaugagc ugguucucac aaauacuaau aggcacacua     1980

cuaauguggc ucggccuaaa cacgaaaaac ggcagcaucu cccuaaugug ccuggcccua     2040

ggaggcguac uaauauuccu aucaacagcc guuuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5136
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 1 opt

<400>  5136
gggagaaagc uuaccaugac aucagucgga aucgucggcc ucuugcuuac aacagccaug       60

gccgcggagg uaacacgccg gggcagcgcc uacuacaugu accuagacag aaacgacgcc      120

ggcgaagcaa ucagcuuccc caccacacuc gguaugaaca aaugcuacau ccaaaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgaau gccccaugcu agacgaaggc      240

gucgaacccg acgacguaga cugcuggugc aacaccacca gcacaugggu ggucuacgga      300

accugccacc acaagaaagg agaagcccga aggagccgcc gcgcagucac ccuacccagc      360

cacucaacaa gaaaacugca gacacgaagc caaacauggc uagaaucccg cgaauacaca      420

aaacaccuaa ucagagucga aaacuggauc uucagaaauc caggcuucgc acucgccgcu      480

gcagcaauag cguggcuacu cggcagcuca acaucaaaga aaguaaucua ccuaguaaug      540

auccuacuga uagcaccagc cuacagcauc agaugcaucg ggguauccaa cagagacuuc      600

gucgaaggca ugagcggagg gacgugggua gacgucgucc ucgaacacgg cggaugcguc      660

accguaaugg cacaagacaa accaacaguc gacauagaac ucguaaccac aaccgucucg      720

aacauggccg aaguccgaag cuacugcuac gaagcgagca ucagcgacau ggcaucagac      780

ucacggugcc caacccaagg agaagcauac cuagacaaac agagcgacac ccaauacguc      840

uguaaaagaa cccucgucga ccgcggaugg ggcaacggcu gcggccuauu cggaaaagga      900

ucacucguaa ccugcgccaa auucgccugc uccaagaaaa ugacaggaaa aaguauucag      960

cccgaaaacc uugaguaccg caucaugcug ucgguccacg gaagucaaca cagcggcaug     1020

aucguaaacg acacuggaca cgagaccgac gaaaaucgag caaaagucga aauaacaccg     1080

aacucacccc gcgccgaagc cacccuuggc ggauucggau cccucggccu agacugcgaa     1140

cccagaaccg gccuagacuu cucggaccuc uauuaucuca cgaugaacaa caaacacugg     1200

cuuguccaca aagaaugguu ccacgacauu ccacuacccu ggcacgcagg agcagacacc     1260

ggcaccccac acuggaacaa caaagaagcc cuaguggagu ucaaagacgc ccacgccaaa     1320

cgccagacag uaguaguccu aggcucccaa gaaggagccg uccacacagc ccuggccggc     1380

gcacuagaag ccgaaaugga cggggcgaaa gggcgccuua guagcgggca ucuuaaaugc     1440

cgccuaaaaa uggacaaacu cagacucaaa ggagucagcu auucccucug cacugcagca     1500

uucaccuuca cuaaaauccc agccgaaacc cuacacggca ccguaaccgu ggaaguacaa     1560

uacgcgggca cagacggccc augcaaagua cccgcccaaa uggccguaga caugcaaacc     1620

cucacaccag ugggcaggcu cauaacagcc aaccccguca ucacggaauc gaccgagaac     1680

aguaaaauga ugcuggaauu agacccgccc uucggagaua gcuacaucgu aaucggagua     1740

ggagagaaga aaaucacaca ccacuggcac cgaucaggca gcacaaucgg caaggcuuuc     1800

gaggccaccg uaagaggcgc gaaacgcaug gcaguacuag gcgacacagc cugggacuuc     1860

ggcucaguug gaggcgcccu aaacagccua ggcaaaggca uccaccaaau cuucggcgcc     1920

gcauuuaaaa gccuauucgg cggaaugucc ugguucagcc agauccugau cggaacucuc     1980

cuaauguggc ugggguuaaa caccaaaaac gggucgaucu cacuaaugug ccucgcacua     2040

ggcggaguac uaaucuuccu gagcacagca gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5137
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 1 opt

<400>  5137
gggagaaagc uuaccaugac auccgucggg aucgucggcc uccuacugac aaccgcuaug       60

gccgccgaag ucacacgcag aggauccgcu uauuacaugu accucgaccg aaacgacgca      120

ggcgaagcga ucaguuuccc cacaacccuc ggaaugaaca aaugcuacau acaaaucaug      180

gaccucggac acaugugcga ugcgacgaug uccuacgaau gccccauguu agacgaaggc      240

gucgaacccg acgauguaga cugcuggugc aacacaacca gcaccugggu aguauacggc      300

acaugccacc acaagaaagg agaggcccgc agaucacgca gagcagucac acuacccagu      360

cacagcacga gaaaacucca aacaaggagc cagacauggc ucgagagccg ggaauacaca      420

aaacaccuaa uacgaguaga aaacuggauc uuccgaaacc caggauucgc gcucgccgcc      480

gccgccaucg ccuggcuccu cggcagcagc accagacaga aaguaauaua ccucgucaug      540

auccuccuaa uagcaccagc auauucaaua cgaugcaucg ggguaagcaa ccgcgacuuc      600

guugaaggga uguccggcgg aacaugggua gaugucgucc uugaacacgg cggcugcgua      660

accguaaugg cccaagacaa acccacggua gacauagagc ucguuacaac aaccguaucc      720

aacauggccg aagucaggag cuacugcuac gaggccagca uauccgacau ggccagcgac      780

agcagauguc ccacacaagg agaagcguac cucgacaaac aaagcgacac acaauacgua      840

ugcaaacgaa cccuagucga cagaggcugg ggaaacggau gcggccucuu cggcaagggc      900

ucccucguaa ccugugcaaa guucgccugc uccaagaaga ugaccggcaa aagcauacaa      960

cccgaaaacc uagaauaucg aaucaugcua agcguacacg ggagccaaca cucaggcaug     1020

aucgucaaug acacaggcca cgaaacagac gaaaacaggg ccaagguaga aaucaccccc     1080

aacagccccc gcgccgaagc cacccugggc ggauucggca gccuaggccu agacugcgaa     1140

cccagaacag gacuagacuu cuccgaccuc uacuaccuca caaugaacaa uaaacauugg     1200

cugguacaca aggaaugguu ccacgacaua ccccucccuu ggcacgccgg agcagacacc     1260

ggaacuccac acuggaacaa caaagaagcc cuaguggagu ucaaggacgc acacgcaaag     1320

agacaaacgg ucguaguacu gggaucacaa gaaggcgcag uccacaccgc ccucgcggga     1380

gcacuagaag ccgaaaugga cggcgcaaaa ggccgacuau ccagcgggca ccuaaaaugc     1440

agacuaaaaa uggacaaacu ccgacuaaaa ggagucaguu acagccuaug caccgccgca     1500

uucacguuca caaaaauccc ggccgagacg cugcacggca ccgucacggu ugaagugcaa     1560

uacgccggca cagacggccc cugcaagguu cccgcccaaa uggccguaga caugcaaacg     1620

cuaaccccag ucggccgccu uauaacggcc aaccccguaa ucaccgaauc aacagaaaac     1680

uccaaaauga ugcucgaacu agacccacca uucggcgaca gcuacaucgu aaucggcguc     1740

ggcgaaaaga aaauaacgca ccacuggcau cgcagcggaa gcacaaucgg caaagcguuc     1800

gaagccaccg uacgcggagc caaacgcaug gccgugcucg gcgacacagc cugggacuuc     1860

ggaucagucg gcggagcccu aaacagccuc ggcaaaggca uacaccaaau auuuggagcg     1920

gccuucaaau cccuauucgg agggaugagc ugguucuccc aaauccugau cggaacccuc     1980

cucauguggc ucggacucaa cacgaaaaac ggcagcauaa gccuaaugug ccuggccuug     2040

ggcgguguac ucauauuucu aagcaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5138
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 1 opt

<400>  5138
gggagaaagc uuaccaugac caguguaggg auaguaggcc uacuccucac aacggccaug       60

gcagcagaag ucacccguag aggaagcgcc uauuacaugu accuagaccg aaacgaugcg      120

ggcgaggcca ucagcuuccc aaccacucua ggcaugaaua aaugcuacau acaaaucaug      180

gaccucggac auaugugcga cgcgacaaug aguuacgaau gccccaugcu agacgagggc      240

guagaaccag acgacgugga cugcuggugc aauacaaccu ccacaugggu cgucuacgga      300

acaugccacc acaagaaagg cgaggcccga cgcucgcgcc gcgcuguaac auuacccucg      360

cacuccaccc gaaaacucca aacacggagc caaacguggc ucgaaagcag agaauacaca      420

aaacacuuaa uaagaguaga aaacuggaua uuucgaaacc ccggcuucgc ccuagccgcg      480

gcagcgaucg cauggcuccu uggaagcagc acaagccaga aagucauaua ccuagugaug      540

auccuccuca ucgcgcccgc auacucaauc cggugcauag gcgucagcaa cagagacuuc      600

guagaaggaa ugagcggcgg caccuggguc gacguagugc ucgagcacgg cggaugcguc      660

accguaaugg cacaggacaa accaaccguc gacaucgaac ucgucacaac cacggucuca      720

aacauggccg aagucagauc guacugcuac gaggccucca ucagcgacau ggccagugac      780

uccagaugcc ccacgcaagg ggaagccuac cucgacaaac aaucagacac acaauacgua      840

ugcaaacgaa cacucgucga caggggaugg ggaaacggcu gcggccucuu cgguaaagga      900

ucgcugguaa ccugcgccaa auucgccugc agcaagaaaa ugaccgggaa aagcauccaa      960

ccugaaaacc uagaauaccg cauaaugcuc ucaguccacg ggucccagca cagcggcaug     1020

auagucaacg acacaggcca cgaaaccgac gaaaacaggg ccaaaguaga aaucaccccc     1080

aacagcccac gcgccgaagc aacgcuaggc ggcuuuggau cccucggacu agacugugaa     1140

ccacgaaccg gccucgacuu cuccgaccua uacuaucuca ccaugaauaa caagcacugg     1200

cucgugcaca aagaaugguu ccacgacauc ccacucccau ggcacgccgg cgccgacacc     1260

ggaacgccac auuggaacaa caaagaagcc cuaguggagu ucaaagacgc acacgcaaaa     1320

aggcaaacag ucgucguacu cggaacucaa gaaggagcag uccacacagc gcucgcagga     1380

gcacucgaag cagaaaugga cggggcaaag ggcagacuau ccagcggaca ccucaagugc     1440

cggcucaaga uggacaaacu acgccuaaaa ggcguaagcu acagccuaug caccgcagcc     1500

uucaccuuca ccaagauacc cgcagaaacc cuacacggca cagucacagu ggaaguccaa     1560

uacgccggca ccgacggacc cugcaaaguc cccgcccaaa uggcagucga caugcagacg     1620

cucacacccg uaggacguuu aaucacagcg aaccccguaa uaaccgaguc aaccgaaaac     1680

agcaaaauga ugcuagaacu cgacccacca uucggcgaca guuacauagu caucggcgua     1740

ggcgagaaaa agauaaccca ccacuggcac cgaagcggga gcacaauagg caaagccuuc     1800

gaagcaaccg uccgaggagc caaaagaaug gccguccuag gagacaccgc cugggauuuc     1860

gggagcgucg gcggggcacu caacagccuc ggaaaaggca uacaccaaau auucggggca     1920

gcauucaaga gcuuguuugg agggauguca ugguucuccc agauccucau cggcacccua     1980

cucauguggc uaggacuaaa caccaaaaac gggucgaucu cacucaugug ccuagcccuc     2040

gggggcguac uuaucuuccu aucaacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5139
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 1 opt

<400>  5139
gggagaaagc uuaccaugac gagcgucggc aucguaggac uacuccucac uaccgccaug       60

gcagccgaag ucacaagacg aggauccgcc uacuacaugu accuagaccg caacgacgca      120

ggcgaagcaa uauccuuccc aaccacgcug gguaugaaca aaugcuacau acaaauaaug      180

gaccucggac acaugugcga cgcaacaaug ucguacgaau gcccgaugcu agacgaagga      240

guagagccag acgacgucga cugcuggugc aacacaaccu ccacgugggu cguguacggc      300

acaugccacc acaaaaaggg agaggcccga agaagccgac gagccguaac ccuccccuca      360

cacucgaccc gcaaacuuca gacacgcagc caaacauggc uagaaagcag agaauacacc      420

aagcaccuga ucaggguaga aaauuggaua uuccgcaacc ccggauuugc ccuagcagcc      480

gcagccaucg cauggcuccu gggauccagc acaucacaga aaguaauaua ccuuguaaug      540

auccuacuaa ucgcaccagc cuacucccuc cggugcauag gcgucagcaa ccgcgacuuc      600

gucgaaggca ugucaggcgg gaccugggua gacguaguac ucgaacaugg gggaugcguc      660

accguaaugg cacaggacaa accaaccguc gacaucgagc ugguaaccac aacaguaagc      720

aacauggcag aagugcgcuc auacugcuac gaagcaucca ucagcgacau ggccagcgac      780

ucccgcugcc caacacaagg cgaagccuac cuagacaaac aaagcgacac ccaauacguc      840

ugcaagagaa cccucguaga ccgaggcugg gguaacggcu gcggacucuu cggcaaaggc      900

ucacucguga cuugcgccaa guucgcaugc agcaagaaaa ugacaggcaa aagcauacaa      960

ccagaaaacc uagaauacag aaucaugcuc agcguucacg gaucccaaca cuccgggaug     1020

aucgucaacg acacaggcca cgaaacagac gaaaaccgcg caaaagucga aaucacacca     1080

aacagcccac gcgcagaagc aacacuuggc ggauucggca gccucgggcu agacugcgaa     1140

ccccgaacgg gccuggacuu cagcgaccua uacuaccuaa ccaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccccucccau ggcacgccgg agccgacaca     1260

ggaacacccc acuggaacaa uaaagaagcc cuaguagagu ucaaagacgc ccacgccaaa     1320

cgccaaacag uaguaguucu agggagccaa gaaggagcag uccacacagc gcucgcaggc     1380

gcccuggagg cagaaaugga cggagccaaa ggacggcucu ccagcggaca ccuuaagugc     1440

cggcuaaaaa uggacaaacu cagacucaaa ggcguaucuu acaguuuaug uaccgccgcc     1500

uuuacauuca ccaagauccc cgcugaaaca cuucacggga ccgucacagu cgaaguccaa     1560

uacgcgggua ccgacggacc augcaagguc cccgcgcaaa uggcaguaga caugcagaca     1620

uuaacacccg uaggccggcu uaucacagca aacccaguaa ucacagaauc aacagaaaac     1680

ucaaaaauga ugcucgagcu agacccgccc uucggggacu cauacaucgu caucggaguc     1740

ggagagaaaa agaucacaca ccacuggcac cgcagcggca gcacgaucgg aaaagcguuc     1800

gaagccacug ugcgcggagc gaaaagaaug gcaguacugg gagacacugc gugggacuuc     1860

ggcucagucg gaggggcacu aaacagccug ggcaaaggaa uacaccaaau auucggcgcg     1920

gcauucaaau cccucuucgg aggaaugagu ugguucucac aaauccugau cggcacccua     1980

cucauguggc ugggucucaa caccaaaaac gggucaauaa gccucaugug ccucgcacua     2040

ggcggaguac uaaucuuccu caguacagca guaagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5140
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 1 opt

<400>  5140
gggagaaagc uuaccaugac cuccgucggg auaguaggcc uacuacucac caccgcuaug       60

gcagcagaag uaacccgaag gggcagcgca uauuacaugu accuagacag aaacgacgcc      120

ggcgaagcga ucaguuuccc gacaacgcug ggaaugaaca aaugcuacau acaaaucaug      180

gaccucggac acauguguga cgccaccaug ucauacgaau gcccgaugcu cgacgaggga      240

gucgaacccg augauguaga cugcuggugc aacacaacaa gcacuugggu cgucuacggg      300

accugccacc acaagaaagg cgaagcacgu cgcagccgca gagcuguaac ccucccaucg      360

cacagcacac gcaaacucca aacccgcucc caaacguggc uggaaucccg cgaguacacc      420

aaacacuuaa ucagaaucga aaauuggauc uuccgcaacc ccggcuucgc acucgcggca      480

gccgccauag cguggcuccu aggcucaucc acgagucaaa aaguaauaua ccucgucaug      540

auccuacuaa uagcccccgc cuacuccaua cgcugcauag gcgucagcaa ccgagacuuc      600

guagaaggaa ugucuggagg caccuggguc gacguaguac uggaacacgg cggcugcguc      660

acggugaugg cacaagacaa accgaccgua gacaucgaac ucgucaccac cacaguaagc      720

aacauggcug agguccgcuc guacugcuac gaggcaagca uaagcgacau ggcaucagac      780

uccaggugcc ccacccaagg agaagccuac cucgacaaac aaagcgacac ccaauaugua      840

ugcaaaagga cccuagucga ccgcgggugg ggaaacggcu gcggacuauu cggcaaagga      900

agccucguua ccugcgcaaa auucgcaugc uccaagaaaa ugaccggcaa aucuauacaa      960

ccagaaaacc ucgaauacag aauaaugcua ucaguccacg gcucacaaca cagcggcaug     1020

aucgucaaug acaccggcca cgaaaccgac gaaaacagag caaaaguaga aaucaccccc     1080

aacagcccac gagccgaggc cacccucggc ggcuucggau cgcuggggcu agacugcgaa     1140

cccagaacag gccucgacuu cucugaccua uacuaccuaa cgaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacauc ccacuaccau ggcacgcagg cgcagacaca     1260

ggaacacccc acuggaauaa uaaagaggca cuugucgagu ucaaagacgc acacgccaaa     1320

agacaaaccg ucguaguccu cggcagccaa gaaggugcug uacacaccgc acucgccggc     1380

gcccucgaag cagaaaugga cggcgcaaaa ggccgccuau caucaggcca ccuaaaaugc     1440

cgccucaaaa uggacaaacu ccgacuaaaa ggcgucagcu acucgcucug caccgcggcc     1500

uucaccuuca ccaaaauccc cgcagaaacc cuacacggaa ccgugaccgu cgaaguccaa     1560

uacgcaggaa ccgacggacc augcaaaguc ccagcacaaa uggccgucga caugcagaca     1620

cuaacaccag uaggcagacu cauaacugcc aacccaguaa ucacagaauc caccgaaaac     1680

aguaaaauga ugcucgaacu agacccgcca uucggcgaca gcuacaucgu caucggcguc     1740

ggggaaaaga aaaucacaca ucacuggcac cgcucaggau ccacaaucgg caaagccuuc     1800

gaagccacag uccgaggggc uaaaagaaug gcaguccucg gcgacaccgc augggacuuc     1860

ggcagcguag gcggcgcccu aaacucacuc ggaaaaggca uucaccaaau auucggcgca     1920

gcauucaaau cacuuuucgg aggaaugagc ugguucucac aaauacuaau cggaacacua     1980

cuaauguggc ucggacucaa caccaaaaac ggguccauaa gccugaugug ucuagcccua     2040

ggaggaguac ucaucuuccu aagcaccgca gucuccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5141
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 1 opt

<400>  5141
gggagaaagc uuaccaugac aucaguggga auaguaggac uccuacuaac gaccgcaaug       60

gccgcagaag uuacccgaag aggcagcgca uacuauaugu accucgaccg caacgacgcc      120

ggcgaagcca ucucauuccc aaccacacuc gggaugaaca aaugcuacau ccaaauaaug      180

gaccuaggac acaugugcga cgcgaccaug ucauacgagu gucccaugcu ugacgaaggc      240

guagaaccgg acgacguaga cugcuggugc aacaccacga gcaccugggu gguauacggc      300

accugccacc acaaaaaagg cgaggcgaga aggagcagac gagccgucac ccuucccagc      360

cauucaaccc gaaaacuaca aacaagaagu caaacguggc uagaaucacg cgaauacaca      420

aaacaccuga uccgcguaga aaacuggauc cuaaggaacc caggauucgc acuggccgcc      480

gcggccaucg ccuggcuccu agggagcagc acaucccaaa aaguaaucua ccuagucaug      540

auccuccuga uagcgcccgc guacagcauc agauguaucg gcgucuccaa ccgagacuuc      600

guagaaggaa ugucuggggg uacauggguc gacgucgucc uagaacacgg cggcugcguc      660

acaguaaugg cacaagacaa accaacggua gacauagaac ucgugaccac caccgucuca      720

aacauggcag aaguacgauc guacugcuac gaggcgucca uaucagacau ggccagcgac      780

agccgcugcc caacccaggg agaggcauac cucgacaaac aauccgacac ccaauacgug      840

ugcaaaagga cacuagucga ccgggggugg gggaacggcu gcggccucuu uggaaaaggc      900

agccucguca caugcgccaa auucgccugc uccaaaaaaa ugacagggaa aagcauccaa      960

ccagaaaacc uagaauaccg aaucaugcuc uccgugcacg gcucacaaca cuccgggaug     1020

auaguaaacg acacaggaca cgagaccgac gaaaaccgag ccaaaguuga aauaaccccc     1080

aacagcccca gagcugaagc cacacuugga ggauucggau cccuaggccu cgacugcgaa     1140

ccacgcacgg gccucgacuu uagcgaccua uacuaccuaa ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacauc ccgcugccau ggcacgccgg cgccgacacg     1260

ggaacccccc acuggaacaa caaagaggca cucgucgaau uuaaagacgc acacgccaaa     1320

cgacaaaccg ucguuguacu aggcucacaa gagggcgcgg uccauacagc acuagcaggc     1380

gcccuggagg ccgaaaugga cggagccaaa ggccgacucu ccagcggaca ucuaaaaugc     1440

cgacugaaga uggauaaacu acgacucaaa ggagucucau acucacuaug uaccgcagca     1500

uuuacauuca caaaaauacc cgcagaaaca cuacacggaa ccguaaccgu cgaaguccag     1560

uacgccggca cggacggccc cugcaaaguc cccgcccaaa uggccgucga caugcaaaca     1620

cugaccccag ucggccgccu cauaaccgca aacccaguca ucacugaauc caccgagaac     1680

agcaaaauga ugcucgagcu cgaccccccc uucggcgaca gcuacaucgu caucggcauc     1740

ggggaaaaga aaauaacaca ccacuggcac cgauccggca gcaccaucgg caaagccuuc     1800

gaggcaacag uccgcggagc aaaacgcaug gcaguccucg gagacaccgc cugggacuuc     1860

ggcagcgucg gaggagcccu caacucucug ggcaaaggga uacaccaaau cuucggagca     1920

gcauucaaaa gccuauucgg cggaaugagc ugguucuccc aaauacucau aggaacccuc     1980

cucauguggc ucggacuaaa caccaaaaac ggauccauaa gccuaaugug ccuagcucuc     2040

ggaggcgugc uaauauucuu aaguaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5142
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 1 opt

<400>  5142
gggagaaagc uuaccaugac cuccguagga aucgucggcc uccugcuaac caccgccaug       60

gcagccgaag ucacacgccg cggcucagca uacuacaugu aucucgacag aaaugacgcc      120

ggugaagcca uaucauuccc caccacacug ggcaugaaua agugcuacau ccaaaucaug      180

gaccugggcc acaugugcga cgccacaaug uccuacgaau gucccaugcu cgacgaaggc      240

gucgagcccg acgacguaga cugcuggugc aacaccacua gcacgugggu cgucuacgga      300

accugccacc acaagaaagg cgaagcccgc cgaucaagaa gggcagucac ccuucccuca      360

cacagcaccc gaaaauuaca aacaagaagc caaacauggc ucgaaagccg cgaauacaca      420

aaacaccuua uccgcguaga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuacu uggcucaucc accagccaaa aagucaucua ccuagucaug      540

auccuacuca ucgcacccgc cuacuccauc cgaugcaucg gcguauccaa ccgcgacuuc      600

gucgaaggaa ugaguggcgg caccugggua gacguugucc ucgaacacgg cggaugcgua      660

accgucaugg cccaagacaa accgaccguc gacauagaac ucgucucaac caccguaagc      720

aacauggcag aaguaagauc auacugcuac gaagcaacca uaagcgacau ugcgucggac      780

ucccgcugcc caacucaggg cgaagccuac cuagacaaac aaucagacac ccaauacgug      840

ugcaaacgaa cccucgugga uagaggcugg ggcaaugggu gcgggcuuuu cgggaaaggc      900

ucccugguca caugcgccaa auucgccugu agcaagaaaa ugacaggcaa auccauccaa      960

ccggaaaacc ucgaauaccg caucaugcua ucaguacacg gcagccaaca cagcggcaug     1020

auuguaaacg acacaggaca cgaaaccgau gaaaaccgag ccaaagucga aauuaccccc     1080

aacucacccc gcgccgaggc aacccuugga ggcuucggcu cccucggacu ggacugcgaa     1140

ccaagaacag gccucgacuu uuccgaccuc uacuaccuaa ccaugaacaa caaacacugg     1200

cucguacaca aggaaugguu ccacgacauc ccccuucccu ggcacgcggg agcggacacc     1260

ggcaccccac acuggaacaa caaagaagcg cuggucgagu ucaaagacgc ccaugccaaa     1320

cgccaaacag ccgucgugcu cgggagccaa gaaggagcag uucacaccgc ccuagccggc     1380

gcccuggaag cagaaaugga cggcgccaaa gggcgacucu ccuccggcca ccucaaaugc     1440

cgacuaaaga uggacaaauu acgccuaaaa ggagucuccu auagccuaug cacagccgca     1500

uucacauuca caaagauacc cgcagaaacc cuccacggca ccguaaccgu cgaaguacaa     1560

uacgcaggaa cggacggacc augcaaagua cccgcccaaa uggcagucga caugcaaacc     1620

cuaacccccg ucggacgacu cauaaccgcc aacccaguca ucacagaaag caccgaaaac     1680

ucaaaaauga ugcucgaacu cgacccaccc uucggcgacu cauacaucgu caucggcguc     1740

ggagaaaaga aaauaaccca ccacuggcac cgcuccggca gcacgaucgg gaaagcauuc     1800

gaagccacag uccgaggagc caagcgaaug gcaguccuag gcgauacagc cugggacuuc     1860

ggcucaguag guggcgcccu aaacucacuc ggaaagggaa uccaccaaau auuuggcgcc     1920

gcauucaaau cccuauucgg cggcaugucc ugguucagcc agauauuaau aggaacacua     1980

cucauguggc uagggcucaa uaccaaaaac ggcuccaucu cgcuaaugug ccuagcccuc     2040

gguggcguac ucaucuuccu cagcaccgca gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5143
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 1 opt

<400>  5143
gggagaaagc uuaccaugac aagcguugga aucguaggac uacuacugac caccgccaug       60

gcugccgaag ucacacgccg aggcucagcc uacuacaugu accucgaccg aaacgacgcc      120

ggugaagcaa uaucauuccc aaccacacuc ggcaugaaca aaugcuacau ccaaaucaug      180

gaccucggac acaugugcga cgccaccaug agcuacgaau gucccaugcu agaugagggc      240

gucgaaccag acgaugucga cugcuggugc aacaccaccu ccaccugggu cguguacggu      300

accugccacc acaagaaagg cgaagcccga cgaucacgac gcgcagucac acucccaagc      360

cacagcacca ggaaacucca aacacgcagc cagacauggc ucgaaagccg cgaauauaca      420

aaacaccuaa uaagaguaga aaacuggauc uuccgaaacc caggcuucgc ccucgcagcc      480

gccgcgaucg cauggcuacu cgggagcuca accucccaaa aagucaucua ccuaguuaug      540

auacuacuaa uagcacccgc uuacuccauc cgaugcauag gcgucucaaa ccgcgacuuc      600

gucgaaggaa ugagcggagg caccugggua gacguagucc ucgagcacgg agguugugua      660

accgucaugg cucaagacaa accaacgguc gacauagagc uaguaacaac aaccgucuca      720

aacauggcag aaguccggag cuacugcuac gaagccagca ucuccgacau ggccucugau      780

ucccgcugcc caacacaagg cgaggcuuac cuagacaaac agucagacac ccaguauguu      840

ugcaaacgga cacucgucga ccgagggugg ggcaacggau gcggccucuu cggcaaaggc      900

ucccucguaa caugcgcaaa auucgcuugu ucaaagaaaa ugacaggcaa aucaauccaa      960

ccggagaacc ucgaauaccg caucaugcuc agcguccacg ggagccaaca cuccggaaug     1020

auagucaacg acaccggaca cgaaaccgac gaaaaccgag caaaagugga aaucacgccu     1080

aacagccccc gggccgaagc aacccuagga ggcuucgggu cccuggggcu ugacugcgaa     1140

ccucguaccg gccuagacuu cagcgaccuc uacuaccuaa ccaugaacaa caaacauugg     1200

cuaguacaca aagagugguu ccacgacauc cccuuacccu ggcacgccgg agcagacacc     1260

ggcacacccc acuggaacaa caaagaagcc cucguugagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg uagugguucu aggaucccaa gaaggagccg uccacaccgc ccucgccgga     1380

gcacucgaag ccgaaaugga cggcgcaaaa ggcaggcuca gcagcggaca ccucaaaugc     1440

cgacuaaaaa uggacaaacu ccgacucaaa ggcguguccu acucccugug cacagccgcc     1500

uucaccuuca ccaaaauccc cgccgaaaca cuccacggca cgguaacagu agaacuacaa     1560

uacgccggga cagauggacc augcaaaguc cccgcacaaa uggcaguaga uaugcaaaca     1620

cuaacccccg uaggccgccu uaucaccgca aaccccguca ucaccgaauc cacagaaaac     1680

uccaaaauga ugcuugaacu cgacccacca uucggcgauu cauacaucgu cauaggcguc     1740

ggagaaaaga aaaucacgca ccacuggcac aggagcggcu ccaccauagg caaagcguuu     1800

gaagccaccg ugcgcggcgc caaacgaaug gcaguccucg gagacacagc cugggacuuc     1860

ggcuccguug gcggggcacu aaacucacuc ggcaaaggca uccaccaaau cuuuggagcc     1920

gcguucaaau cacucuucgg uggcaugucu ugguucucuc agauccuaau cggcacucuu     1980

cucauguggc uaggacucaa uaccaaaaac ggcucgauca gccucaugug ccucgcacuc     2040

ggcggcguac ucauuuuccu cagcacagcc gucucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5144
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 1 opt

<400>  5144
gggagaaagc uuaccaugac auccguagga aucgucggac uccugcucac aaccgccaug       60

gccgccgaag ucacaagacg aggcuccgca uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcaa ucagcuuccc aaccacccuc ggcaugaaca aaugcuacau acaaauaaug      180

gaucuaggac acaugugcga cgccacgaug agcuacgaau gccccaugcu ggacgaagga      240

guggagccag acgacgucga cugcuggugc aacaccacaa gcacaugggu uguauacgga      300

accugccacc acaagaaagg cgaggcccgc cgcagcagac gagcaguaac ucuaccaucc      360

cacuccacac gcaaacucca aacucgaucc cagaccuggc ucgaaagccg agaauacaca      420

aaacaucuca uacgcgucga aaacuggaua uucagaaacc caggguucgc acucgccgcc      480

gccgccaucg ccuggcuccu aggcagcagc acaucccaaa aagucaucua ccuaguaaug      540

aucuuauuaa uagcccccgc guacuccaua cggugcaucg gaguaucaaa ccgagacuuc      600

guagaaggaa ugagcggggg cacguggguc gacguugucc ucgaacacgg cggcugcgua      660

accguaaugg cccaagacaa accaacagua gacauagaac ucgucaccac aaccgucagc      720

aacauggccg aagugcgcuc auacugcuac gaagccucca uuuccgacau ggccucugac      780

aguaggugcc caacgcaggg agaagcauac cuagacaaac aaagcgacac ccaauacguc      840

ugcaagcgca cucuagucga ccgaggaugg ggaaacgggu gcggucuauu cggaaaaggc      900

agucuuguaa ccugcgcaaa auucgcuugc uccaagaaaa ugacaggcaa aagcauacaa      960

cccgaaaacc ucgaauaccg caucaugcuc uccguccacg gcucacaaca cucagggaug     1020

aucgucaaug acacaggcca ugaaaccgac gaaaaccggg ccaaagucga aaucaccccc     1080

aacagcccca gagccgaagc aacccuagga ggcuucggca gccuaggccu cgacugcgaa     1140

ccucgcaccg gucuggacuu cuccgaucuc uacuaccuca ccaugaacaa caaacauugg     1200

cucguccaca aagaaugguu ccacgacauc ccccucccau ggcacgccgg agccgacaca     1260

ggaacaccac auuggaacaa caaagaagca cuggucgagu ucaaagaugc ccaugccaag     1320

agacagaccg ucgugguccu cggcucacaa gaaggcgccg uacacaccgc acucgccgga     1380

gcgcucgaag ccgaaaugga cggagccaaa gguaggcuaa gcuccggaca ccugaaaugc     1440

agacuuaaaa uggacaaguu acgccucaaa ggcgucucau acucccuuug cacagccgca     1500

uucacuuuca caaaaauccc cgccgaaacc uuacacggga caguaacugu ggaagugcaa     1560

uacgccggca ccgacggacc gugcaagguc cccgcgcaaa uggcagucga caugcaaaca     1620

cuaacacccg uaggccgccu gaucacagca aacccuguua ucacagaauc aauagaaaac     1680

uccaaaauga ugcuugaacu cgacccaccg uucggagaca gcuacaucgu aauaggcguc     1740

ggcgaaaaga aaauuaccca ccacuggcac cgcucaggca gcacaaucgg aaaagccuuc     1800

gaagccacag uccggggcgc caaacgaaug gccguccucg gcgacaccgc augggacuuc     1860

ggaucaguag gaggcgcacu caacagucuu gggaaaggaa uccaccaaau auucggagcc     1920

gccuucaaga gccucuucgg aggcaugucc ugguuuagcc aaauacuaau aggcacacuc     1980

cuaauguggc uaggccucaa caccaagaac ggcagcauca gccucaugug ccucgcccuc     2040

ggaggaguac uaauauuccu auccaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5145
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 1 opt

<400>  5145
gggagaaagc uuaccaugac aaguguagga aucguggguc uccuccugac aaccgcaaug       60

gccgcagaag uaacacggcg gggguccgca uacuacaugu accucgaccg gaacgacgca      120

ggcgaggcca ucucuuuccc caccacccua ggcaugaaca aaugcuacau ccaaauaaug      180

gaccuaggac acaugugcga cgcaaccaug uccuacgaau gccccaugcu agaugaagga      240

gucgaaccag acgacgugga cugcuggugc aacaccacca guaccugggu cgucuacgga      300

acaugccacc acaagaaagg ggaggcucgc agaagccgcc gagcugucac acucccaagc      360

cacagcacca gaaaacucca aacacgaucc caaacauggc uagaaucgcg cgaguacacc      420

aaacaccuca uccgcgugga aaacuggauc uuccgaaacc caggcuucgc ccucgcagcg      480

gcagcaauag cauggcuguu aggauccucc acaagccaaa aaguaauaua ccugguaaug      540

auccuacuca ucgcacccgc auacuccauc cgaugcauag gaguauccaa ccgggacuuc      600

gucgaaggaa ugagcggagg aaccuggguc gacguggucc uagaacacgg cggaugcguc      660

accgucaugg cacaagacaa acccacagug gacaucgagc uaguaaccac caccguguca      720

aacauggcug aaguuagauc cuacugcuac gaagcaagca ucagcgacau ggcgagugac      780

agcagaugcc ccacacaagg cgaagcauac cucgacaaac aauccgauac ccaauacgua      840

ugcaaacgca cacucguaga ccgcgguugg ggcaacggcu gcggauuauu cgggaaaggg      900

agccucguua ccugcgcaaa auucgccugc agcaaaaaga ugacaggcaa aagcauccaa      960

cccgagaauc uagaauacag aauaaugcuc uccguacacg gaucccaaca cucagggaug     1020

auaguuaaug acacaggcca cgaaacagac gaaaaccgag ccaaaguuga aauaaccccg     1080

aacucaccaa gagccgaagc cacccuagga ggcuucggau cucucggacu cgacugcgaa     1140

ccccgcacag gacuagacuu cuccgaccua uacuaucuca cuaugaacaa caagcacugg     1200

cucguacaua aagaaugguu ccacgacauc ccacuacccu ggcacgcggg cgcagacaca     1260

gggacgcccc acuggaacaa uaaagaagcg cucguagagu ucaaggaugc ucacgccaag     1320

cgccaaacag ucguaguacu aggcucccaa gaaggcgccg uccacacagc ccuagcagga     1380

gcccuggaag ccgaaaugga cggaguaaag ggcagacucu cuagcgggca ccuuaaaugc     1440

cgacugaaaa uggacaaacu cagguuaaag ggcguauccu auucacugug caccgcggca     1500

uucacauuca ccaaaauccc ggccgaaaca cuccacggca caguaacggu cgaaguccaa     1560

uacgccggca cggacggacc cugcaaagua cccgcccaaa uggcagucga caugcaaacc     1620

cucacacccg ucggaaggcu aauaacagcc aaccccguca ucaccgaaag caccgaaaac     1680

agcaaaauga ugcucgaacu agacccaccc uucggcgacu cauacauagu gaucggcguc     1740

ggcgaaaaga aaaucacgca ccacuggcac agaucaggau cgacaauagg aaaagccuuc     1800

gaagcgacag uccgcggagc aaaacgaaug gccguauuag gggacacagc augggacuuc     1860

ggcuccgucg gcggcgcacu caacucccuc gguaaaggaa uacaccaaau auucggugca     1920

gcuuucaaau cacuauucgg cggcaugucc ugguucagcc aaauucucau cggaacacuc     1980

cucauguggc ucggccuuaa cacuaaaaau ggcucaaucu cguucaugug ccucgcccua     2040

ggcggagucc ugaucuuccu cuccaccgcg guauccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5146
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 1 opt

<400>  5146
gggagaaagc uuaccaugac aagcguagga auagucggac uacuacucac aacggcaaug       60

gcagccgaag ucacccgaag agggagcgcc uauuacaugu accuagacag aaacgacgca      120

ggagaagcaa ucucauuccc cacgacacuc gggaugaaca aaugcuacau acaaauaaug      180

gaccuaggac auaugugcga cgcgacaaug ucauacgaau gcccaaugcu cgacgaagga      240

guggaacccg acgacguaga cugcuggugc aacaccacau ccaccugggu gguauauggc      300

acaugccacc acaagaaagg cgaagcacgc cgaagccgcc gagcagucac acucccaagc      360

cacagcaccc gcaaacucca gacaagaagc caaacauggc uagaaucccg agaauacacu      420

aaacaccuaa uacggguaga aaacuggaua uucagaaacc caggauucgc acuugcagcc      480

gcagccaucg cuuggcuccu aggaagcucc acgucccaaa aagugaucua ucucgucaug      540

auacuacuga ucgcccccgc cuacagcaua cgcugcauag gcguaagcaa ccgagauuuc      600

guggaaggaa ugagcggcgg caccugggua gacgucguuc uagaacacgg aggcugcgua      660

accguaaugg cccaagacaa acccaccgug gacaucgaac ugguuacaac caccguaucc      720

aacauggccg agguuagaag cuacugcuac gaagcgucca ucucagacau ggccucagac      780

ucacgcugcc ccacacaagg agaagcauac cucgacaaac aaucagauac gcaguacguc      840

ugcaaaagaa cacuggugga ccgcggcugg ggaaacggau gcggccuguu cgguaaaggc      900

agccucguca caugcgcaaa auucgcgugc ucgaaaaaga ugacaggcaa aagcauacaa      960

cccgaaaacc uagaauaccg aaucaugcua uccguccacg gcucacaaca cucuggaaug     1020

aucgucaacg acacgggcca ugaaacggac gaaaacagag ccaaagucga gauaacaccc     1080

aacagcccca gggccgaagc gacacucgga ggcuucggua gccugggacu agacugcgaa     1140

ccgcgcacag gccuggacuu cagcgaccuu uacuaccuga ccaugaauaa caaacacugg     1200

cucguccaca aggaaugguu ccacgacauc ccccugccau ggcaugccgg cgcagacacc     1260

ggcaccccac acuggaacaa caaagaagcc cuaguagagu ucaaagacgc acacgcaaaa     1320

cgccagacag uaguaguccu gggcucacag gaaggcgcag ugcacaccgc acuggcaggc     1380

gcgcuggaag ccgagaugga cggcgccaaa gguaaacuau cuucgggcca ccucaaaugc     1440

cgucuaaaaa uggacaaacu aagacuaaaa ggggucuccu acagccucug caccgcagcc     1500

uucaccuuua caaaaauacc agcagaaaca cuccacggga caguaaccgu cgaagugcag     1560

uacgccggca cagacggccc cugcaagguc cccgcacaga uggcagucga caugcagaca     1620

cucaccccag uaggacgccu cauaaccgca aaccccguca ucaccgaaag caccgagaac     1680

aguaaaauga ugcucgaauu ggacccacca uucggagaca gcuacaucgu aauaggaguu     1740

ggcgaaaaga aaaucacaca ccauuggcac agaagcggga gcaccauagg caaagcauuc     1800

gaagccacgg uccgaggcgc aaaacggaug gcagugcuag gcgacaccgc augggacuuu     1860

ggaagcgugg gcggagcacu gaacagucua ggaaaaggca uacaccagau auucggagca     1920

gcguucaaaa gccuguuugg cggaauguca ugguucucgc aaauccucau aggcacccua     1980

cuuauguggc uagggcucaa caccaaaaau ggaagcauca gccuaaugug ccuagcacua     2040

gggggcgucc uaauauuccu cagcaccgcc guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5147
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 1 opt

<400>  5147
gggagaaagc uuaccaugac gagcgucgga aucgucggac uccuccuaac caccgcaaug       60

gcggccgaag ucacccgccg aggcagcgca uacuacaugu accuagaccg aaaagacgcc      120

ggagaagcga ucagcuuccc caccacacua ggaaugaaca aauguuacau acaaaucaug      180

gaucugggac acaugugcga cgccaccaug agcuacgaau gccccaugcu cgacgaaggc      240

guagaaccgg acgacguaga cugcuggugc aacaccacca gcaccugggu cguauacgga      300

accugccacc acaagaaagg agaagcacgg cgaagccgac gcgccgucac acuccccuca      360

cacagcaccc gcaaacucca aacccgaucc caaaccuggc ucgagagcag agaauacacg      420

aaacacuuaa uacgaguaga gaacuggaua uuccgcaacc ccggauucgc acuagcagca      480

gccgccaucg ccuggcuacu cggcucaucc acaucacaaa aagucaucua ccuggucaug      540

auacuacuaa uagcacccgc cuacagcaua cgcugcauag gaguaagcaa ccgcgacuuc      600

gucgaaggaa uguccggcgg caccugggua gacguagucc uggagcacgg cggcuguguc      660

accguaaugg cacaagacaa acccaccgua gacaucgagc uaguaacaac caccgucagu      720

aacauggcag aaguacgauc cuacugcuac gaagcaagca ucuccgacau ggcgucagac      780

ucccgguguc ccacgcaagg agaagcauac cucgacaagc aaagcgacac acaauacguc      840

ugcaaacgaa cccucgucga ccgaggaugg ggcaacgguu gcggccuguu cggaaaagga      900

ucccucguga ccugcgcaaa auucgcaugc agcaagaaaa ugaccggcaa gagcauccaa      960

cccgaaaacc uagaauaccg caucaugcug ucaguccacg gcagccaaca cagcgggaug     1020

auagucaacg acacuggcca cgaaaccgac gaaaacagag caaaagugga gauaacccca     1080

aacagccccc gagccgaggc aacacucgga ggauucggga gccucggccu cgacugcgaa     1140

cccaggaccg gacucgacuu cagcgaccuc uacuaccuga ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacaua ccucugccuu ggcacgcagg cgccgacacg     1260

ggaacuccac acuggaacaa caaagaagcc cucguagagu ucaaggacgc ccacgcgaaa     1320

aggcaaacgg uaguuguacu aggcagucaa gaaggcgcag uacacaccgc ccuagccggc     1380

gcccuagaag ccgaaaugga cggcgccaaa ggccgucuau ccagcggaca cuuaaagugc     1440

cgacuaaaaa uggacaaacu ucgccucaaa ggggucucau acagucucug caccgcugcg     1500

uuuaccuuca ccaaaauccc cgcagaaaca cuccacggaa cugucacagu cgaagugcaa     1560

uacgccggga cagacggccc cugcaaagua ccagcacaga uggccgucga caugcagacc     1620

uuaacaccug ucggaagacu caucacagcc aaccccguca uaaccgaauc cacagaaaac     1680

agcaaaauga uguuagaacu cgacccuccc uucggagacu cauacauagu aaucggcgug     1740

ggcgaaaaga aaaucaccca ccacuggcac agauccggau ccaccaucgg aaaagccuuc     1800

gaagccaccg uccgaggcgc caaacgcaug gccguauugg gagacacagc augggacuuc     1860

ggguccguag gaggcgccuu aaacucgcua gggaaaggca uccaccaaau cuucggagcc     1920

gccuucaaaa gccucuucgg uggcaugagc ugguucuccc aaauccucau cggcacgcuc     1980

cuaauguggc uaggucugaa caccaagaac ggcucaauau cccuaaugug ccucgcacua     2040

ggcggagucc uaauauuccu aucaaccgcc gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5148
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 1 opt

<400>  5148
gggagaaagc uuaccaugac cuccguggga aucguaggac ugcuacucac aacagccaug       60

gcagcagaag ucacccgaag aggcucugcg uacuacaugu accuagaccg caacgacgcc      120

ggggaagcga uaucauuccc aacaacacuc ggcaugaaca aaugcuacau ccaaauaaug      180

gaccuaggcc acaugugcga cgccacaaug agcuacgaau gcccaaugcu cgacgaagga      240

guggagccag augaugugga cugcuggugc aacacgaccu caaccugggu aguauacggc      300

acaugccacc acaaaaaggg cgaggcccgc agaagccgca gagcagucac ccuacccuca      360

cacucuacaa gaaaacuaca aacacgcucc caaacauggc uagaaagcag agaauacaca      420

aaacaccuaa uacgaguaga gaacuggauc uuccgaaacc caggcuucgc auuggcagcc      480

gccgcaaucg cuuggcuacu aggguccagc accagccaaa aagucaucua ccuaguuaug      540

auacuccuca ucgccccagc cuacagcauc cgaugcauug guguaucaaa cagggacuuc      600

guagaaggaa ugagcggcgg aacaugggua gacguggucc ucgaacacgg cggcugcgua      660

acgguaaugg cccaagacaa acccacagua gauauagagc uagucacaac gacaguaagc      720

aacauggccg aaguaagaag cuacugcuac gaagcaagca uaagcgacau ggccucggac      780

uccaggugcc ccacgcaggg cgaagcauac cuagacaagc agagugacac ccaauacguc      840

ugcaagcgca cgcuaguaga cagaggaugg ggaaacgguu gcggccuauu cggaaaaggg      900

ucacucguga ccugcgcaaa guucgccugc aguaaaaaga ugacaggcaa aagcauacaa      960

cccgagaacc uggaauaccg uaucaugcuc agcguacacg gcucacaaca uagcgggaug     1020

auagucaacg acacaggcca cgaaacggac gagaacagag caaaagucga aauaacccca     1080

aacucacccc gagcagaagc aacccuaggc ggcuucggau cccugggacu agacugcgaa     1140

cccagaacag gccuugacuu uuccgaucug uauuaccuga caaugaacaa caagcacugg     1200

cuaguacaca aagaaugguu ccacgacaua ccccuacccu ggcacgcagg cgccgacacc     1260

ggcacacccc acuggaacaa caaagaagcc cucguagagu ucaaagaagc ccacgcaaag     1320

cgccaaacgg uagucguacu ggggagccaa gagggagccg uacacacagc gcuagcggga     1380

gcacucgaag cagaaaugga cggggccaaa gggagacuua gcagcggcca ccuaaaaugc     1440

cgacuaaaaa uggacaaacu ccgccuaaag ggaguaucau acucccugug cacagcagca     1500

uucaccuuca ccaagauccc cgcagaaaca cuccauggca cagucacagu agaaguacag     1560

uacgccggca ccgacggacc cugcaaagua ccagcacaaa uggccguaga caugcaaaca     1620

cuaaccccag uaggcagacu caucacagcu aauccaguaa uaacagaaag caccgagaac     1680

aguaaaauga ugcuggagcu cgacccgccc uucggcgacu ccuacaucgu aauaggagug     1740

ggcgagaaaa agauaacaca ccauuggcac agaagcggca gcaccauagg caaagcauuc     1800

gaagcgacgg ugcgcggggc uaaacgcaug gcagugcuag gcgacaccgc gugggacuuc     1860

ggcucaguag gaggggcacu aaacucacuc ggaaaaggca uacaccagau cuucggagca     1920

gccuuuaaga gccucuucgg aggcaugagc ugguucucgc aaauacucau cggcacacuu     1980

cucauguggc ucgggcuaaa caccaaaaac ggaucaauca gccucaugug ccuagcacua     2040

ggaggggucc ucaucuuccu aagcacggcg gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5149
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 1 opt

<400>  5149
gggagaaagc uuaccaugac cagcguugga auagucggcc uacuacuaac cacagcaaug       60

gccgccgaag ucacacgcag aggguccgcu uacuacaugu accuagaccg aaacgacgcg      120

ggcgaagcaa ucagcuuccc caccacccuc ggaaugaaca aaugcuacau ccaaaucaug      180

gaccuaggac acaugugcga cgcgacuaug aguuacgaau gccccaugcu cgacgaagga      240

gucgaaccgg acgacguaga cugcuggugc aacaccacgu ccaccugggu aguauacgga      300

accugccacc acaagaaagg cgaagcacgc cgaagccgcc gcgccgucac gcugccaucu      360

cacucaacac gaaaacuaca aacacgaagu cagacauggc uagagagccg cgaguacacc      420

aaacaccuga uaagaguuga aaauuggaua uucagaaacc caggcuucgc gcuggcggca      480

gcagccaucg ccuggcuccu cggaucaucc accagccaga aagucauaua ccuaguaaug      540

auccuccuca ucgcaccagc guacagcauc cgcugcaucg gcguaagcaa ccgcgauuuc      600

guagaaggca ugagcggagg caccugggua gacguaguac ucgaacacgg aggaugcgua      660

accgucaugg cccaagacaa acccacaguc gacaucgaac ucgucaccac cacaguaagc      720

aacauggccg agguccgcag cuacugcuac gaggcaagca ucagcgacau ggcaucggac      780

ucccgaugcc caacccaagg ggaagcguac cuagauaaac aaagcgacac acaauacguc      840

ugcaaacgca cacuuguaga ccgaggaugg gguaacggau gcggacucuu uggcaaaggc      900

agccucguca caugcgccaa auucgcaugc ucaaagaaaa ugacaggaaa auccauccag      960

cccgagaacc uggaguaccg caucaugcuc aguguccacg ggagccaaca cucaggcaug     1020

aucgucaacg acacgggcca cgaaaccgac gaaaaccgag ccaaagucga aaucaccccc     1080

aacucgccaa gagcagaagc cgcccucggc ggcuucggau cacucggacu ggacugcgaa     1140

cccagaacug gacucgacuu cucagaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacaua ccccuacccu ggcacgccgg ugcugacaca     1260

ggcacuccac acuggaacaa caaagaagcc cuaguagagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg uagucguacu agggagccaa gaaggcgccg uacacaccgc acuagcaggc     1380

gcacuagaag cagaaaugga cggcgcaaaa ggcagacugu cuagcggcca cuuaaaaugc     1440

cgucucaaaa uggacaaacu gagacucaaa ggaguaagcu acucccuaug cacagcagca     1500

uucacauuca caaaaauacc cgcagaaaca cuacacggca caguaaccgu cgagguacaa     1560

uacgcaggga cagacgggcc cugcaaaguc ccagcacaaa uggccgugga uaugcaaacc     1620

cucacacccg uaggacgacu aauaaccgca aacccaguca uuacagaauc caccgaaaac     1680

agcaaaauga ugcucgaacu agacccacca uucggcgacu cauacaucgu cauaggaguc     1740

ggggaaaaga aaaucacaca ccacuggcac cgcagcgggu cgaccaucgg caaagccuuc     1800

gaagcgacag uuagaggggc aaaaaggaug gccguccuag gcgacaccgc gugggacuuc     1860

ggcuccguag gcggagcccu aaacagucuc ggcaaaggaa uacaccaaau cuucggagca     1920

gcguucaaau cacuauuugg cggcaugucu ugguucucac aaacccuaau cgggacccuc     1980

cucauguggc ucggccuaaa caccaaaaac ggcuccauca gccucaugug cuuagcccuc     2040

ggcggggucc uaauauuccu uuccacagca gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5150
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 1 opt

<400>  5150
gggagaaagc uuaccaugac cagcguagga aucgucggcc uccuacuuac caccgcaaug       60

gccgcagaag ucacccgucg aggcucagcc uacuacaugu accucgacag aaacgacgcc      120

ggcgaagcca ucagcuuucc gacaacgcuu ggcaugaaca aaugcuacau acaaauaaug      180

gaccucggcc acaugugcga cgcaacgaug ucauaugaau gcccaaugcu agacgaaggc      240

guagaaccag acgacgucga cugcuggugu aacacaacau ccaccugggu ggucuacgga      300

accugccacc acaagaaagg agaagcucga cgaagccgac gcgccguaac acugcccagc      360

cauuccacgc gcaaacucca aacacgcucc caaaccuggc uugagagccg agaguacaca      420

aaacaccuaa uacgcgugga aaacuggaua uuccgaaacc ccggcuucgc acucgccgca      480

gccgcaauag cguggcuucu aggaagcucc accucacaga aagugauaua ucugguaaug      540

auacugcuca uagcacccgc cuacagcaua agaugcaucg gcgucucaaa cagggacuuc      600

gucgaaggaa ugagcggagg gacguggguc gacgucgucc uagaacacgg gggcugcgua      660

acagucaugg cgcaagacaa gcccacggua gacaucgaac uggucaccac cacagucuca      720

aacauggccg aaguacgcuc cuauugcuau gaagccucaa ucucagacau ggcguccgac      780

agccgcugcc ccacccaagg cgaagccuac cucgacaaac aaucggacac ccaauacguc      840

ugcaaaagaa cacuuguaga caggggaugg ggcaacggau gcggacucuu cggcaagggc      900

agccuaguaa caugcgccaa auucgccugc ucaaagaaaa ugaccggaaa guccauccaa      960

cccgaaaacc uagaauacag aaucaugcua uccguacacg gcagccaaca cuccggcaug     1020

aucgucaacg acaccggcca cgaaacagac gaaaaccgag ccaaggucga aauaaccccc     1080

aacucaccca gggcggaagc aacacuuggc ggauucggca gccucggccu agacugcgaa     1140

ccacgcaccg gccuagacuu cagcgaccuc uacuaccuaa caaugaacaa caaacacugg     1200

cucguacaua aggagugguu ccaugacaua ccgcuacccu ggcacgcagg cgccgacacc     1260

ggaaccccac acuggaacaa caaagaagcg cucgucgagu ucaaagacgc ccacgccaaa     1320

cgccagaccg uagucguccu aggaucacaa gaaggcgcug uacacacagc ccucgccgga     1380

gcccucgaag ccgaaaugga cggagcaaaa ggccgccuca gcagcgggca ccuaaaaugc     1440

cgacuaaaaa uggacaaacu ccgucucaaa ggaguaagcu acucccucug cacagccgcu     1500

uucaccuuca caaaaauacc agccgaaacc cuccacggca ccgucacagu agaaguccaa     1560

uacgcagggg cugacggacc cugcaaaguc cccgcccaaa uggccguaga caugcaaacg     1620

cucaccccag ucggcagacu uauaaccgcg aaccccguaa ucaccgaaag caccgaaaac     1680

ucaaaaauga ugcucgagcu agacccaccc uucggcgaca gcuacaucgu caucggcgug     1740

ggagaaaaga agaucacaca ccacuggcac agaagcggcu cgacaauagg caaagcauuc     1800

gaagccaccg uacguggcgc gaaacgcaug gccgugcucg gagacaccgc uugggacuuc     1860

gggagcguag ggggagcccu caacucccuc ggcaagggaa uacaccaaau auucggcgcu     1920

gccuucaaga gccucuucgg cggaaugagu ugguucuccc aaauacucau cggaacucuc     1980

cuaauguggc ucggacucaa caccaaaaac ggcucgauuu cccucaugug ccuugcacuu     2040

ggcggugucc uuauauuucu cuccaccgcc guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5151
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 1 opt

<400>  5151
gggagaaagc uuaccaugac cagcgucggc auuguaggcc uauuacuuac aacagcuaug       60

gccgccgaag ucacaagacg gggauccgcc uacuacaugu accuagaccg caacgacgcc      120

ggagaagcca uaucauuccc cacaacccua ggcaugaaca agugcuacau acaaauaaug      180

gaccuugguc acaugugcga cgccacaaug agcuacgaau gucccaugcu cgacgaaggc      240

guagagcccg acgacgucga cugcuggugc aacacaacau ccacgugggu aguauacggg      300

accugccacc acaagaaagg cgaggccaga cggucacgac gagccguaac ccuacccucc      360

cacuccaccc gaaaacucca aacacgcagc caaacuuggc uagaaucacg agaauacacc      420

aaacaccuaa uccgcguaga gaacuggaua uucagaaacc caggauucgc acuagccgcc      480

gccgcaauag ccuggcuccu aggcucaucg accucacaga aaguaaucua ccucguaaug      540

auacuucuca ucgcacucgc cuacagcauc cgcugcaucg gcguaagcaa ucgcgacuuc      600

gucgagggaa ugucaggagg aacauggguc gacgucgugc uagagcacgg cgggugcgua      660

acggucaugg cacaagacaa acccaccguc gacaucgaac ucguaacgac cacagucucu      720

aacauggccg aaguacgaag uuacugcuac gaagccucca uaucggacau ggcaagcgac      780

ucccgcugcc cgacccaagg agaagccuac cuggacaaac agagcgacac acaauacgug      840

ugcaaacgaa cucuagucga ccgcggaugg ggcaacggau gcggccucuu cggaaaagga      900

agccuaguaa caugcgcgaa auucgcuugc uccaagaaaa ugaccgggaa auccauccaa      960

ccugagaacc uggaauaucg aauaaugcua uccguacacg gcucccaaca cuccggcaug     1020

aucgucaacg acacuggaca ugaaacagac gaaaaccgcg ccaaagugga aaucacaccc     1080

aacuccccac gcgcggaagc aacgcucgga ggauucggcu cacuaggccu agacugcgaa     1140

cccagaaccg gacuagacuu cagcgaccuc uacuaccuca caaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccccuaccau ggcacgcagg ggcggacacg     1260

ggaacacccc acuggaacaa caaagaagcu cucguagagu ucaaagacgc gcaugcgaaa     1320

cgacaaaccg uaguaguccu cggaucgcaa gaaggagcag uacacaccgc ccuggccggc     1380

gcccuagaag ccgaaaugga cggagcaaaa ggccgacuca gcagcgggca ccucaaaugc     1440

agacuaaaaa uggacaaacu aagacucaaa ggcguaagcu acagccuuug cacagcagcc     1500

uuuacauuca caaaaauucc cgcugaaaca cuccacggca ccguaaccgu agaaguccaa     1560

uacgcaggca ccgacggacc augcaaaguc ccagcacaga uggcugucga caugcaaaca     1620

cucacacccg uaggacgacu aauaacugcc aaccccguga ucaccgaaag cacagaaaac     1680

agcaagauga ugcucgaacu ggacccaccc uucggcgacu ccuauaucgu cauuggaguc     1740

ggagaaaaga aaaucaccca ccacuggcac cgcagcggcu ccacaaucgg caaggccuuc     1800

gaagcaaccg uaagaggcgc caaaagaaug gccguacuag gagacacggc augggacuuc     1860

ggcagcguag gcggggcacu caacagucua ggaaagggca uccaccaaau auucggcgca     1920

gccuucaaau cacuauucgg cggcaugagc ugguucaguc aaauacucau aggcacgcug     1980

cuaauguggc ucggccucaa uaccaaaaac ggaucgaucu cccucaugug ccucgcccuu     2040

ggaggcgugc uaaucuuccu auccaccgca gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5152
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 1 opt

<400>  5152
gggagaaagc uuaccaugac cuccguagga auagucggcc uccuccugac cacagccaug       60

gccgccgaag ugaccagacg cggcucagca uacuacaugu accucgacag gaacgacgcc      120

ggcgaagcca ucucauuccc gaccacauua ggcaugaaca aaugcuacau ccaaaucaug      180

gaucuaggcc acaugugcga ugccaccaug ucauacgaau gccccaugcu cgaugaaggc      240

gucgaaccgg acgacguaga cugcuggugc aacaccaccu ccaccugggu cguauacgga      300

accugccacc acaagaaagg ugaagcacgc agaagccgac gcgccgugac acuucccuca      360

cacuccaccc gaaaacuaca aacacgcuca caaaccuggc uagaaucccg cgaauacacc      420

aaacaccuca uacgcgucga aaacuggauc uuucgaaacc caggauucgc ucucgccgca      480

gccgcaaucg cauggcuucu cggaucuucc acaucccaaa aagucaucua cuuaguuaug      540

auccuccuca ucgccccagc cuacuccaua cgaugcaucg gcguaagcaa ccgcgacuuc      600

gucgaaggca ugucgggagg cacgugggua gacgucguuc uagaacacgg uggcugcgua      660

acaguaaugg cccaagacaa gcccaccguc gacauugaac ucgucaccac cacuguauca      720

aacauggcug aaguccgcag cuacugcuac gaagcaagca ucuccgacau ggucuccgac      780

agccgcugcc caacccaagg cgaagcguac cuagacaaac agucggacac ccaauacguc      840

ugcaaacgca cccuagucga cagagguugg ggcaacggau gcggacucuu cggcaaggga      900

ucccuaguaa caugugccaa auucgcgugc ucgaagaaaa ugaccggaaa aagcauccag      960

cccgaaaacc ucgaauaccg cauaaugcuc uccguccacg gaagccaaca cuccggcaug     1020

aucgugaacg acaccggaca cgaaaccgac gaaaaccgcg cuaaaguaga aaucacacca     1080

aacucacccc gcgcagaagc aacccuagga ggcuucggau cccucggccu agacugugaa     1140

ccccggaccg gccuagauuu cuccgaccuc uacuaccuaa ccaugaacaa caaacacugg     1200

cucguacaua aagagugguu ucacgacauc ccccuccccu ggcacgcggg cgcagacaca     1260

gguacacccc auuggaacaa caaagaagcc cuugucgagu ucaaagacgc acacgccaaa     1320

agacaaaccg ucgucgugcu uggcucccag gaaggagccg ugcacacagc ccuggccgga     1380

gcgcuagaag cagaaaugga cggcgccaaa ggaagacuaa gcuccggaca ccuaaaaugc     1440

cgacuaaaaa uggacaaacu ucgacuuaaa ggagugucau acucacucug cacggccgca     1500

uuuaccuuca ccaaaauccc cgcagaaaca cuacacggca ccgucaccgu cgaaguacaa     1560

uacgccggaa ccgacggccc augcaaggua ccagcacaaa uggccgucga caugcagaca     1620

cuuaccccgg uaggccgacu gauaaccgcu aacccaguca ucaccgaauc aaccgaaaau     1680

ucgaaaauga ugcuggaacu cgacccaccc uucggagacu cauauaucgu cauaggcgua     1740

ggagaaaaga aaaucaccca ccacuggcac cgcucuggca gcacaauagg aaaagccuuc     1800

gaagccacag ucagaggcgc caaacgcaug gccgugcucg gcgacaccgc cugggacuuc     1860

ggaagcgucg gaggcgcccu caacagccug ggcaaaggaa uccaccaaau auucggagca     1920

gccuuuaaau cccuuuucgg aggaauguca ugguucuccc aaauucuaau uggcacacuc     1980

cuaauguggc ucggacuaaa caccaaaaau ggcucaauaa gccugaugug ucuagcacua     2040

ggaggcguac ucaucuuccu uagcaccgcc gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5153
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 1 opt

<400>  5153
gggagaaagc uuaccaugac cuccguaggc auagucggcc uacuccugac aaccgcaaug       60

gcagccgaag ucacacgccg cggaagcgcc uacuacaugu accucgaccg aaaugacgcc      120

ggcgaagcca ucagcuuccc caccacgcuc ggcaugaaca aaugcuacau acaaaucaug      180

gaccucggcc acaugugcga cgccaccaug ucguacgaau gccccaugcu agacgaaggc      240

gucgaaccag acgacguaga cugcuggugc aacaccacau cgacaugggu aguguacgga      300

acaugccacc auaagaaagg agaagcaaga cgcagcaggc gagcggucac ccuacccagc      360

cacuccacga gaaaacucca gacacgcucc caaacauggc ucgaaagcag agaauacacu      420

aaacaccuca uccgaguaga aaauuggaua uuuagaaacc caggcuucgc gcuagcggca      480

gccgcaaucg ccuggcuacu cgguaguucc acaucacaaa aaguaaucua ccucguaaug      540

auccuccuaa uugcaccagc auacuccaua agaugcaucg gcaucuccaa cagagacuuc      600

guggaaggca ugucaggggg aacaugggua gacguuguac ucgagcacgg cggcugcgua      660

accguaaugg cccaagacaa accaacagug gacaucgaac ucguuaccac aaccgucagu      720

aacauggccg aaguccguuc cuacugcuac gaggccagua uauccgacau ggccagcgac      780

agccgcugcc caacccaagg ggaagccuac cucgacaaac aaucagacac ccaauauguu      840

ugcaaaagaa cccuagucga ccgaggcugg ggaaacggau gcggacuuuu cggcaaaggc      900

ucgcucguca caugcgccaa auucgcaugc uccaagaaaa ugaccggcaa aagcauacaa      960

ccagaaaacc ucgaauaccg caucaugcuc uccguacacg guagccaaca cagcgggaug     1020

aucgucaacg acacaggcca cgaaaccgac gaaaaccgcg cgaaagucga aauaaccccu     1080

aacucacccc gugccgaagc aacacuuggc ggguucggua uccugggccu cgacugcgaa     1140

cccagaaccg gccucgacuu cuccgaccuc uacuaccuua ccaugaauaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacaua ccgcuccccu ggcacgccgg agccgacaca     1260

ggcacacccc acuggaacaa caaagaagcc cugguagagu ucaaggacgc gcacgcaaaa     1320

cgacagaccg uagucgugcu cggcagucaa gaaggugcug ugcacacggc acucgccggu     1380

gcacuggaag cagaaaugga cggagcaaaa ggccgccuau ccagcggcca ucucaaaugc     1440

cgacucaaaa uggacaaacu acgucuaaaa ggagugucgu acagccuaug cacagccgcc     1500

uucacauuca cgaaaauccc agccgaaaca cuacacggaa cgguaacagu agaaguacaa     1560

uaugcaggca ccgacggacc augcaaagua cccgcccaaa uggccguuga caugcaaacu     1620

cuaacuccag uaggacgccu gauuaccgcc aacccaguca ucaccgaaag caccgaaaac     1680

ucuaaaauga ugcucgaacu ggacccacca uucggugaca gcuacaucgu cauaggcgua     1740

ggagaaaaga aaaucaccca ccacuggcac cgcagcggcu ccaccauagg caaagcauuc     1800

gaagcaaccg uccggggagc caaacgcaug gcaguccucg gcgacaccgc cugggauuuc     1860

ggguccgucg gcggggcccu caauucccug gggaaaggaa uacaccaaau cuucggggcc     1920

gccuucaaaa gccuuuucgg cggaauguca ugguucucuc aaauacucau cggaacccua     1980

cuaauguggc uuggccugaa caccaaaaac ggaagcaucu cacucaugug ccucgcacuu     2040

ggaggcgugc ucaucuuccu auccaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5154
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 1 opt

<400>  5154
gggagaaagc uuaccaugac aagcguaggc aucguaggac uccuccuaac aaccgccaug       60

gcagcagaag uuacacgccg gggaucagca uacuacaugu accuagaccg caacgacgcc      120

ggugaagcca ucucguuccc cacaacacug ggcaugaaca aaugcuacau ccaaaucaug      180

gaccucggac auaccugcga cgcaacaaug ucauaugaau gccccaugcu agacgaaggc      240

gucgaacccg acgacguaga cugcuggugc aacaccacca gcacgugggu agucuauggc      300

accugucacc acaagaaagg cgaagcacgc cguucucgca gagcagucac ccuacccuca      360

cacagcaucc gcaaacuuca aacccgcucc caaacauggc ucgagucccg ggaauauacg      420

aaacaccuca uacgaguaga aaacuggauc uuccgcaacc ccggcuuugc ccuagccgca      480

gcagcaauag ccuggcuauu aggcuccagc accagccaaa aaguaaucua ccuggucaug      540

auccuccuca uugccccugc auacucaaua cgcugcaucg gagucucaaa cagggauuuc      600

gucgagggaa ugucgggcgg aacguggguc gacgucgucc ucgaacacgg uggcugcgug      660

acagucaugg cccaagacaa accgacaguu gacauagaac uugucaccac aacaguauca      720

aacauggcgg aaguccgcag cuacugcuac gaagccucaa uauccgacau ggcaagcgac      780

agccgaugcc caacccaagg cgaagcauac cucgacaaac aauccgacac gcaauaugua      840

ugcaaacgca cccuaguaga ccggggcugg ggaaacggcu gcggacucuu cggcaaaggc      900

agccuaguca caugcgcuaa auucgccugu agcaagaaaa ugacaggcaa aagcauccaa      960

cccgaaaauc ucgaguaccg aaucaugcua agcguacacg gcucacaaca cucaggaaug     1020

auagucaacg acacaggaca cgagacagac gaaaaccgcg caaaagucga gauaacgccc     1080

aacuccccac gcgcagaagc aacacucggc ggcuucggaa gucucggucu agacugcgaa     1140

ccccgaacag gacuagacuu cucagaccua uacuaccuga cuaugaacaa caagcacugg     1200

cucguccaca aagaaugguu ucacgacauc cccuuaccau ggcacgcagg cgcugacacc     1260

ggcacaccac acuggaacaa caaagaagca cucgucgagu ucaaagacgc ucacgccaaa     1320

cggcaaaccg ucguuguccu gggcucccaa gaaggcgcgg uacacaccgc ccucgccggc     1380

gcucucgagg cagaaaugga cggagcaaaa ggacgccucu ccaguggaca ccucaaaugc     1440

agacuaaaaa uggacaaacu acgccucaaa ggugucucau acagccucug uacagcagca     1500

uucaccuuca ccaaaauccc ggccgagaca cuccacggaa ccguaaccgu agaaguccaa     1560

uacgcaggga cagacggacc cugcaaaguc ccagcacaaa uggcaguaga caugcaaaca     1620

cuaacacccg ugggaagacu caucaccgca aaccccguca ucaccgaaag caccgaaaac     1680

agcaaaauga ugcuagaacu agacccaccc uucggcgacu cauacauagu aauaggcgug     1740

ggagaaaaga aaaucaccca ccacuggcac cgcagcggau caaccauagg caaagcauuc     1800

gaagccacag uccgcggugc aaaacgcaug gccguccucg gcgacacagc cugggacuuc     1860

ggcagcguag gcggcgcccu aaacucgcua ggaaaaggca uacaccagau cuucggggca     1920

gccuucaaga gucucuucgg cgguaugucc ugguucagcc aaauccucau cgggacccua     1980

cucauguggc ucggccucaa cgccaaaaac ggcucgaucu cacucaugug ccuagcacuc     2040

ggcggcgucc ucauauuccu gucaacagcc guaagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5155
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 1 opt

<400>  5155
gggagaaagc uuaccaugac aucagucggg auagucggcc uacuacuaac cacggccaug       60

gcagccgaag uaacccgacg cggaucggca uacuacaugu accuagacag aaacgacgca      120

ggagaagcca uaucuuuccc caccacauua ggcaugaaca aaugcuacau ccaaaucaug      180

gaccuaggac acaugugcga cgcaacgaug ucguaugagu gccccaugcu cgacgaaggg      240

guggaacccg acgacguaga cugcuggugc aacacaaccu caaccugggu cguauacgga      300

accugccauc auaagaaagg ggaagcccaa cgcucccgca gagccguaac acuacccagc      360

cacagcacca gaaaacuaca aacccgcuca caaaccuggc uggaaucacg cgaguacacc      420

aaacaccuaa uaaggguaga aaacuggaua uuccggaacc caggcuucgc ccucgccgca      480

gcagcaauag cauggcuacu cggauccuca acaucccaga aagugaucua ccucguuaug      540

auacuccuua ucgcccccgc cuacagcauc cgaugcauag gcgucagcaa ccgagacuuc      600

guagaaggaa ugagcggcgg caccugggua gacgucguac ucgagcacgg cgguuguguc      660

accgucaugg cccaggacaa accaacagua gacauugaac ucgugacaac aacggucucc      720

aacauggcag aaguacgcag cuacugcuac gaagccagca ucagcgacau ggccuccgac      780

agccgcugcc ccacacaagg agaagcguac cuggacaaac aaucagacac ccaauacguc      840

ugcaaacgca cccuagucga ccgaggaugg ggcaacggau gcggccucuu cggcaaagga      900

ucgcucguaa caugcgccaa auucgccugc agcaagaaaa ugaccggaaa aucaauccaa      960

cccgaaaacu uagaauaccg cauaaugcua ucaguccacg gcucacaaca cagcggcaug     1020

aucguaaacg acaccggcca cgaaaccgau gagaaccgcg caaaggucga aauaaccccc     1080

aacagccccc gcgccgaagc aacccuagga ggauuuggaa gccucggccu agacugcgaa     1140

ccacgcaccg gccucgacuu cuccgaccua uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aagaaugguu ccacgacauc ccccuccccu ggcacgccgg agccgacacg     1260

ggaacacccc acuggaauaa caaagaggcc cuaguagagu ucaaagacgc acacgcgaaa     1320

cggcaaacag uaguuguacu cggaucacaa gaaggggccg uacacacagc ccuagcagga     1380

gcacucgaag cggaaaugga cggagccaaa ggcagacuca gcagcgggca ucuaaaaugc     1440

cgacucaaaa uggacaaacu ccgauugaaa ggcgucagcu acagccucug uaccgcagca     1500

uuuacauuca ccaagauacc cgccgaaaca cuacacggaa caguaaccgu agaaguccaa     1560

uacgccggga cagacgggcc cugcaaggua cccgcacaga uggcagugga caugcaaacc     1620

uuaacaccgg uaggacgacu gaucacagcg aacccaguca ucacggaauc aaccgaaaac     1680

uccaaaauga ugcuggaacu agacccgccu uucggcgaua gcuacaucgu aauuggagug     1740

ggcgaaaaga aaaucacaca ccacuggcac cgcuccggcu caacaaucgg aaaagccuuc     1800

gaagccaccg uccgcggcgc caaaagaaug gcaguccuag gagacacagc augggacuuc     1860

gguagcguag gcggagccuu aaacucacug ggaaaaggca uccaccaaau cuucggagcc     1920

gcauucaaau cacuauucgg aggaaugucc ugguucaguc aaauacucau cggcacgcua     1980

cucauguggc uaggacucaa caccaaaaac ggaagcaucu cgcugaugug ccuagcccua     2040

ggaggcguac ucauauuccu auccacagcg gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5156
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 1 opt

<400>  5156
gggagaaagc uuaccaugac aagcgucgga auagugggac uccuacucac aacagcaaug       60

gcggccgagg ucacacgacg aggaagcgcc uacuacaugu accuagaccg caacgacgca      120

ggagaggcca ucucguuccc cacgacccua ggcaugaaca aaugcuacau ccagaucaug      180

gaccucgguc acaugugcga cgcaacaaug ucguacgaau gccccaugcu cgacgaaggg      240

gucgaaccag acgacguaga cugcuggugc aacacaacaa gcaccugggu gguauacggc      300

accugucacc acaagaaagg agaagcccga agaagccgca gagccguaac acucccaagc      360

cacuccaccc ggaagcuaca ggcacgcagc caaaccuggc uagaaagcag agaauacacc      420

aaacaccuca uacgcgucga aaacuggaua uucagaaacc ccggauucgc ccuagccgcc      480

gccgccaucg cguggcuuuu agguuccucc acuagccaaa aaguaauaua ccuaguaaug      540

auacuccuaa uagcgccagc cuacagcauc agaugcauag gcguauccaa cagagacuuc      600

gucgaaggca uguccggagg aacauggguc gaugucguac uagaacacgg cggcugcgua      660

accgucaugg cccaagacaa accaacagua gauauagaac ucgucacuac cacgguaagc      720

aacauggccg aggugcgcuc auacugcuac gaggccagca ucucagacau ggcaucagac      780

ucacgcugcc caacacaagg agaagcauac uuagacaaac aaagcgacac acaauacgua      840

ugcaaacgca cccuaguaga uagaggcugg ggcaacgggu gcggacucuu cggcaaagga      900

ucccuaguca caugcgcgaa auucgccugc ucaaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaacc ucgaauaccg caucaugcua agcgugcacg gaucacaaca cuccggcaug     1020

aucguaaacg acacaggcca cgaaacagac gaaaaccgag caaaagugga aauaacaccu     1080

aacucaccac gcgccgaagc cacccuaggg ggcuucggcu cacucggccu cgacugcgaa     1140

cccaggacag gccuggacuu uucggaccug uacuaccuua caaugaacaa caaacacugg     1200

cuaguacaua aagaaugguu ccacgacauu ccacuucccu ggcacgcagg agccgacacc     1260

ggcaccccac acuggaacaa uaaggaagca cucguagagu ucaaagacgc ccacgccaag     1320

agacagacag uagucguacu cggaucccag gagggcgcag uacacacggc ccuagcgggc     1380

gcacuagaag ccgagaugga cggcgccaaa ggccggcuca gcagcggcca ccucaaaugc     1440

cggcuuaaaa uggacaagcu cagacugaaa ggcguaucau acagccuaug caccgccgcc     1500

uucacguuca caaaaauccc agcggaaaca cuacacggaa ccgucacagu cgaagugcaa     1560

uacgcgggca cagacggccc cugcaaagug cccgcccaaa uggcggucga caugcaaaca     1620

cuaaccccag uaggaagacu caucaccgca aacccgguca uaacagaaag cacagaaaac     1680

agcaagauga ugcuagaacu agacccacca uucggcgacu cauacauagu caucggcgua     1740

ggagaaaaga aaaucacaca ccacuggcac cgaagcggcu cuacaaucgg aaaagccuuc     1800

gaagccaccg uacgcggcgc gaaacgcaug gccguccucg gagacacagc augggacuuu     1860

ggcagcguag gaggcgcccu caacucccua ggcaaaggca uccaccaaau auucggggcc     1920

gccuucaaau cccucuucgg aggaauguca ugguucagcc agaucuuaau aggaacccug     1980

cuaauguggc uaggccucaa cacgaaaaac ggguccauau cgcuaaugug ccuagcacug     2040

ggcggagucc ucauauuccu aucaacagcc guauccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5157
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 1 opt

<400>  5157
gggagaaagc uuaccaugac aagcgucggc aucguaggcc uccuccugac cacagccaug       60

gcagccgaag uuacacgccg aggaucagca uacuacaugu accucgaccg cuccgacgcg      120

ggagaagcca ucagcuuccc uaccacccuc ggcaugaaca aaugcuacau acaaauaaug      180

gaccugggac acaugugcga cgcaacaaug ucguacgaau gcccaaugcu agacgaaggc      240

gucgaacccg augacguaga cugcuggugc aacaccacau ccaccugggu ggucuacgga      300

accugccacc acaaaaaggg cgaggccaga cggucgagac gcgcggucac ccuuccuagc      360

cacagcacca gaaaacuaca aacacggucg cagacuuggc ucgaaaguag agaguauacc      420

aaacaccuca uccgggucga aaauuggauc uuccgcaacc ccggauucgc gcucgccgcc      480

gccgcaauag cguggcuacu cggcagcucc acaagccaaa aagucauaua ccucguuaug      540

auacuccuca ucgcccccgc cuacuccauu cgaugcauag gcguaagcaa cagagacuuc      600

guagaaggca ugaguggagg gaccuggguc gacguagucc ucgaacacgg cggcugcguc      660

accguaaugg cgcaagacaa acccacagua gacaucgagc uagucaccac caccguaagu      720

aacauggcag aaguccgauc cuacugcuac gaagcaucaa ucuccgacau ggccuccgau      780

agccggugcc ccacacaagg cgaagcauac cucgacaagc aaagcgacac ucaguacguc      840

ugcaaaagaa cacucgucga ccgaggcugg ggaaauggau gcggccuauu cgguaaaggc      900

ucccucguaa ccugcgccaa auucgcaugu ucgaagaaaa ugaccggaaa aagcauacaa      960

cccgagaacc uagaauaccg uauaaugcua agcguccacg gaagccaaca cagcggaaug     1020

aucgucaacg acacaggcca ugaaacagac gaaaaccgcg ccaaaguaga aauaaccccu     1080

aacucaccaa gggcagaagc aacccuaggc ggauucggaa gccuaggacu cgacugugaa     1140

ccaaggacag gacucgacuu cucagaccua uacuaccuca ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacauu ccgcuaccgu ggcaugcugg cgccgauaca     1260

ggcacgcccc auuggaacaa caaagaagca cucguagagu ucaaagacgc ccacgcaaaa     1320

cgccaaaccg ucgugguccu agggagccag gaaggagccg uccacaccgc ccucgccgga     1380

gcccucgaag ccgaaaugga cggcgcaaaa ggccgcuuaa gcuccggcca ccuaaaaugc     1440

cgccugaaaa uggacaaacu caggcucaaa ggcguaagcu acucgcuaug uaccgcagca     1500

uucacauuca gcaaaauccc agcagaaaca cuacacggca cagucacaga agaaguacag     1560

uacgccggca ccgacggccc cugcaaaguc cccgcgcaaa uggccgucga uaugcaaaca     1620

cuaacacccg uaggccgacu aauaacagca aaccccguaa ucaccgaaag caccgaaaac     1680

agcaaaauga ugcuggaacu cgacccgccc uucggagacu cauacaucgu aauaggcguc     1740

ggagaaaaga aaaucacuca ccacuggcac cgcuccggca gcaccaucgg caaagccuuc     1800

gaagccaccg uccgcggcgc caagcgaaug gcgguacuag gcgacacagc cugggacuuc     1860

ggaucaguug gaggagcccu aaacucacua ggcaagggca uacaccaaau cuucggagca     1920

gccuucaaau cccuauucgg gggcaugagc ugguucagcc aaauccuaau cggaacccuc     1980

cucauguggc ucggccuaaa caccaaaaac ggcuccauua gucuaaugug ccuagcccuc     2040

ggcggcgugc ucaucuuccu auccacagcg guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5158
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 1 opt

<400>  5158
gggagaaagc uuaccaugac cucagucgga auaguaggac uacuccucac gacagcaaug       60

gccgcagaag uaaccagacg cgguuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggagaagcca ucagcuuccc cacaacccua ggcaugaaca aaugcuacau acaaaucaug      180

gaucucggac acaugugcga cgccaccaug uccuacgaau gccccaugcu cgacgaagga      240

guagagccgg acgacguuga cugcuggugc aacacaacau ccacgugggu agucuacgga      300

accugccauc acaagaaagg cgaagcccga agaucccgcc gcgccgucac acuacccagc      360

cacucaacgc ggaaacucca aacaagaucg caaaccuggc uagaaagcag agaauauacc      420

aaacaccuaa ucagaguaga gaacuggaua uucaggaacc caggcuucgc acuagccgcc      480

gcagccauag ccuggcuccu cgguuccucc acaagccaaa aaguaauaua ccucgucaug      540

auacuccuca ucgcaccagc auauucgaua cguugcauag gagugagcaa uagagacuuc      600

guagagggaa ugucaggagg caccugggua gacgucguac ucgagcacgg cggaugugua      660

acaguuaugg cccaagacaa acccaccguc gacauagaac ucgucacaac cacagucuca      720

aacauggccg aaguaagauc cuacugcuac gaggcuucca ucucagacau ggcauccgac      780

ucacgcugcc caacccaagg cgaagcguau cucgacaaac agucugacac ccaguacguc      840

ugcaaacgca cacucgugga cagaggaugg ggcaacggau gcggccucuu cggcaaaggc      900

ucccuaguaa caugcgccaa auucgcaugc uccaagaaaa ugaccggaaa aagcauacag      960

cccgaaaacc ucgaauaccg cauaaugcua ucaguccacg gaucccaaca cuccggcaug     1020

aucguaaacg acaccggcca cgaaaccgac gaaaaccgcg cgaaagucga aaucaccccc     1080

aacagcccac gagccgaagc cacacuaggu ggcuucggaa gccucggacu ggacugugaa     1140

ccccgcacag gccuagacuu cagcgaccuu uacuaccuca ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccacuaccgu ggcacacagg agcagacacc     1260

ggaacaccac acuggaacaa caaagaagcc uuaguagagu ucaaagacgc ccacgcaaaa     1320

cgacaaacag ucguaguacu cggcucucaa gaaggagccg uccacaccgc acuagcaggc     1380

gcccucgaag ccgaaaugga cggagcuaaa ggcaggcuga gcucgggcca ccuuaaaugc     1440

agacucaaaa uggacaaacu uagacucaaa ggaguaucau auucccucug cacagcagca     1500

uucaccuuua ccaaaauacc agccgaaacc cuacacggaa ccgugaccgu agaaguucaa     1560

uacgcaggaa ccgacggccc augcaaagua cccgcacaaa uggcagugga caugcagacc     1620

cugacaccgg ucggacgccu cauaacagcc aaccccguca ucacugaagg cacagaaaac     1680

ucgaaaauga ugcucgaacu agacccaccc uucggcgacu ccuacauagu caucggcguc     1740

ggugaaaaga aaaucacaca ccacuggcac agaagcggcu caacaaucgg aaaagccuuc     1800

gaagccaccg uccgcggugc gaaaagaaug gcaguccucg gcgacacagc augggauuuc     1860

ggaagcguag gcggaguacu aaacucgcuc ggcaaaggca uacaccaaau cuuuggcgca     1920

gccuucaaaa gccuauucgg aggcaugucc ugguucagcc agauccuaau cggcacacuc     1980

cuaauguggc ugggacucaa uaccaaaaac gggagcauau cccucaugug ccuugcacua     2040

ggcggcguuc ucaucuuccu cuccacagcc gucagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5159
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 1 opt

<400>  5159
gggagaaagc uuaccaugac cagcgucggu auagucggcu uacuccucac aacagcaaug       60

gcagccgagg ucacccgaag aggaucagcc uacuacaugu aucuggaccg aucagacgca      120

ggggaagcaa ucuccuuccc aacaacccua ggcaugaaua agugcuacau acaaauaaug      180

gaccucggac acaugugcga cgccacgaug uccuacgaau gcccaaugcu ugacgagggc      240

guagaaccag acgacgucga cuguuggugc aacaccacgu ccacgugggu cgucuacgga      300

accugccacc auaagaaagg cgaagcgcga cguagcagac gagcagucac ucuaccaagc      360

cacagcacca gaaaacuaca aacaagaagc caaaccuggc uagaaagccg agaguacacc      420

aaacaccuaa uccgcguaga aaacuggauc uucaggaacc cuggcuucgc acucgccgca      480

gcagcaaucg ccuggcugcu cggaucaagc acaagccaaa aaguaauaua ccuugugaug      540

auccugcuaa uagcccccgc cuacuccaua cgcugcauag gagucucaaa ucgcgacuuc      600

guugaaggca uguccggcgg cacauggguc gacguagugc ucgaacacgg uggcugcguc      660

acagucaugg cccaggacaa accaacagua gacauagaac uuguaaccac uaccgucagu      720

aacauggccg agguacguuc auacuguuau gaagcaucca uaucggacau ggccucagac      780

ucccgaugcc caacccaagg cgaagcguac cucgacaaac aaucagacac acaauauguc      840

ugcaaacgca cccuagucga cagaggcugg ggaaacggau gcggacucuu cggaaaaggc      900

ucccucguaa ccugcgcaaa auucgcgugc ucaaagaaaa ugacgggcaa aagcauacaa      960

cccgaaaacc ucgaguaccg caucaugcua ucaguccacg guucacagca uagcggcaug     1020

auaguaaacg acacaggaca cgagacagac gagaaccgcg caaaagucga gauuacaccu     1080

aauagucccc gcgcagaagc cacauuaggc ggauucggaa gccugggacu agacugcgaa     1140

ccucgaaccg gacucgacuu cuccgaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacauc cccuuaccau ggcacgccgg cgcggacaca     1260

ggcaccccac acuggaacaa caaagaagcc cuagucgagu ucaaagacgc ccacgccaaa     1320

agacaaacug uagucguccu aggaagccaa gaaggagcag ugcacaccgc acuggcaggc     1380

gcccuagaag cagaaaugga cggcgccaaa ggcagacuga gcucaggcca ccuaaaaugc     1440

cgacucaaaa uggauaaacu ccgauuaaaa ggggucucau acucccucug caccgccgcc     1500

uucacuuuca caaaaauccc cgccgaaacc cuccacggua ccgucaccgu cgaaguacaa     1560

uacgccggca ccgacggccc cugcaaaguc cccgcccaaa uggccguaga caugcaaacc     1620

uuaaccccag ucggacgccu caucacagca aaucccguca ucaccgaauc caccgaaaac     1680

ucgaaaauga ugcucgaacu agacccacca uucggagaca gcuacaucgu caucggagua     1740

ggugaaaaga aaauaaccca ccacuggcac cgcagcggaa gcaccauagg aaaagccuuc     1800

gaggccaccg uccgaggagc aaaaagaaug gcgguucucg gugacaccgc augggacuuc     1860

ggcagcguag gcggagcucu gaacagccua ggaaaaggaa uccaccaaau uuucggggcc     1920

gcauucaaaa gccucuucgg aggaaugucc ugguucucgc aaauacucau uggcacacuc     1980

cucauguggc ucggacucaa cacaaagaac ggcagcauca gccucaugug ccucgcccuu     2040

gguggcgucc ucauauuccu cagcacagcc gucucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5160
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 1 opt

<400>  5160
gggagaaagc uuaccaugac cagcgugggc auagucggac uccuacucac caccgccaug       60

gccgucgaag ucacgcgccg gggcaacgcc uacuacaugu accuagaccg uagcgacgca      120

ggagaggcaa uaucauuccc cacaacaaug ggaaugaaca aaugcuacau ccagaucaug      180

gaccuaggcc acaugugcga cgcaacaaug ucauacgaau gcccaaugcu cgacgaagga      240

guggaacccg acgacguaga cugcuggugc aacacaacaa gcaccugggu aguauacgga      300

acgugccacc acaagaaagg cgaagcccga cggucccggc gagccgucac ccucccaagc      360

cacuccacac gaaaauuaca aacaaggagc caaaccuggc ucgaaagccg agaguacacc      420

aaacaccuca ucagagugga aaacuggauc uuccgaaacc cuggauucgc gcucgccgca      480

gcagccauag cauggcuucu cggcuccagu accagccaga aaguuaucua ccucguaaug      540

auccuacuca ucgcuccagc auacuccauc cgaugcaucg gcgucagcaa ccgcgauuuc      600

gucgaaggua ugagcggcgg cacaugggua gacgucguac uagaacacgg cggcugugug      660

acagucaugg cucaggacaa accaacaguc gauaucgaac ucguaaccac aaccgucucc      720

aauauggcag aagucagauc auauugcuac gaagcaagca ucuccgacau ggccagcgac      780

ucacguugcc caacccaagg cgaagccuac cuggacaaac aguccgacac ccaauacgua      840

ugcaaacgaa cccucguuga ccgaggcugg ggcaacggcu gcggccucuu cggcaaagga      900

ucacucguaa caugcgccaa auucgcuugc ucaaagaaaa ugacgggaaa aagcauacaa      960

cccgaaaacc uagaguaccg uauaaugcuc uccguccaug gaucccaaca cucaggcaug     1020

aucgucaacg acacgggcca cgaaacagac gaaaaccgcg caaaagucga aauaacaccc     1080

aacagccccc gagccgaagc aacacucgga ggcuucggua gccucggccu agacugugaa     1140

ccgcgcacag gacuagacuu uagcgaccuc uacuacuuaa ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacaua ccauugcccu ggcacgcagg ggccgacaca     1260

ggcaccccac acuggaacaa caaagaagcc cucguagagu ucaaagaugc acacgcuaaa     1320

agacaaaccg ucgucguacu cggcagccaa gaaggcgccg uacacacagc ccuugcgggc     1380

gcacuagaag cagaaaugga cggagccaaa ggccgacugu cauccggcca ccucaagugc     1440

agacucaaaa uggacaaacu ccgccuaaaa ggcguuuccu acagccucug caccgccgcc     1500

uucacauuca ccaagauucc cgcggaaacu cuccauggga ccgucacagu agaaguacaa     1560

uacgcaggaa cagacggccc cugcaaaguc cccgcccaga uggccgucga caugcaaacc     1620

cugacccccg ucgggcgucu aaucacagcc aacccgguaa ucaccgaauc gaccgaaaac     1680

ucaaaaauga ugcuagaacu ugacccgccc uucggcgacu ccuacaucgu cauaggcgua     1740

ggcgaaaaga aaauaaccca ucacuggcac cgauccggua gcaccauagg gaaagccuuc     1800

gaagccaccg uacgcggagc aaaaagaaug gccguccucg gagacacagc augggacuuc     1860

ggaucgguag gcggagcacu caauucacua ggcaaaggca uacaccagau cuucggcgca     1920

gcauucaaaa gccucuucgg cggaaugagc ugguucagcc aaauccuaau aggaacccua     1980

cugguuuggc uaggccucaa caccaaaaac ggcucaauca gccucaugug ccuugcccua     2040

ggaggcgucc ucauauuucu aucaacugca gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5161
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 1 opt

<400>  5161
gggagaaagc uuaccaugac cuccguaggc auagucggac uccucuugac cacagccaug       60

gcagccgaag ucacccgacg cggcagcgca uacuacaugu accuagaccg uuccgacgca      120

ggcgaagcaa uuagcuuccc caccacccua ggcaugaaca aaugcuacau acaaauaaug      180

gaucucgggc acaugugcga ugccacaaug ucguacgaau gccccaugcu cgacgaagga      240

guagaacccg acgacgucga cugcuggugc aacacaacca guacaugggu agucuacgga      300

accugccacc acaagaaagg ugaagcucgg agaucccgac gcgcggucac ccuccccucc      360

cacuccacaa gaaaacugca aacaagguca caaacauggc ucgaaagcag agaauacacc      420

aagcaccuaa uaagagugga aaacuggaua uuucggaacc ccggcuucgc guuagccgca      480

gccgccaucg cguggcuccu agggagcuca acuucacaaa aaguaauaua ccucgucaug      540

auccuccuga uagccccagc cuacuccaua cguugcauag gcguaucuaa ccgcgacuuc      600

gucgaaggaa uguccggagg cacauggguc gacgugguac ucgaacaugg cggcugcgua      660

accguaaugg cacaagacaa accgacggua gacauagaac ugguuacaac caccgucagc      720

aacauggccg aaguaagauc auacuguuac gaagcaucaa ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaagg agaagcguau cucgacaagc aauccgacac ccaauaugua      840

ugcaaacgca cccucgugga ccgaggaugg ggcaacggau gcggccuauu cggcaaagga      900

ucccuaguca caugcgcgaa guucgcaugc ucaaagaaaa ugaccggaaa auccauacaa      960

cccgagaauc ucgaauacag aaucaugcuc uccguacacg ggucucaaca uuccggcaug     1020

aucgugaacg acacuggcca cgaaaccgau gaaaaccgag caaaagucga gauaacacca     1080

aauucacccc gcgccgaagc cacccuagga ggcuucggaa gccuaggccu cgacugcgaa     1140

ccccgaaccg gacuggacuu cuccgaccug uacuaccuca caaugaacaa caaacacugg     1200

cuaguccaca aagagugguu ccacgacaua ccacuucccu ggcacgccgg cgccgauaca     1260

gggacacccc acuggaacaa caaagaagcc cuuguagagu ucaaagacgc acacgcaaaa     1320

cgccaaacag ucgugguccu aggcagccaa gagggagccg uacacaccgc ccuagccgga     1380

gcccuagaag ccgaaaugga cggcgccaaa ggcagauuaa gcuccggcca ccucaagugc     1440

cgacuaaaaa uggauaaacu gcgccuuaaa ggcguaagcu acucccucug uacagcagca     1500

uucacauuca caaaaauacc cgccgagaca cuccacggaa caguaacagu cgaaguccaa     1560

uacgcaggaa cagauggacc cugcaaaguc ccggcacaaa uggccguaga caugcaaacc     1620

cuaacccccg ucggcagacu cauaaccgca aaccccguca uaacagaguc aaccgaaaac     1680

ucaaaaauga ugcuggaacu cgacccaccc uucggcgaca gcuacauagu aauaggcgua     1740

ggcgaaaaga aaauaacaca ccacuggcac cgcagcggau caaccaucgg gaaagccuuc     1800

gaagccacgg uccgaggcgc aaaaagaaug gccgugcucg gcgacacagc cugggacuuc     1860

ggcagcgucg gcggagcccu caacucccua ggcaagggca uucaccaaau cuucggagcc     1920

gccuucaaaa gccucuucgg aggcaugucg ugguucuccc aaauacuaau cggcacccuc     1980

cuuauguggc ugggccucaa caccaaaaac ggcuccaucu cgcuagucug ccuagcccuc     2040

gggggagucc ucauauuccu uucgacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5162
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 1 opt

<400>  5162
gggagaaagc uuaccaugac cucaguugga auaguggggc uacuacuaac cacagccaug       60

gcggcagaag uaacccgaag aggcagcgcc uacuacaugu accucgaccg cagcgacgcc      120

ggcgaagcaa ucagcuuccc aaccacccua ggaaugaaca aaugcuacau ccaaaucaug      180

gaccuagguc acaugugcga cgccaccaug agcuacgaau gccccaugcu ugacgaagga      240

guugaaccag acgacgucga cugcuggugc aacaccacaa gcaccugggu cgucuacggg      300

accugccacc acaagaaagg cgaagcgcgc cgaagccgca gagcugucac ccuccccagc      360

cacuccaccc gaaaacuaca aaccaggagc caaacauggc ucgaaagccg cgaauauacc      420

aaacacuuaa ucagagugga gaacuggaua uuccgcaacc ccggauucgc acucgcagcg      480

gcagcgaucg cauggcuccu aggcagcucc accagccaaa aagucaucua cuuagucaug      540

auccuccuca uagcccccgc cuacucgauc agaugcaucg gcguuaacaa ccgagacuuc      600

gucgaaggca ugucuggcgg caccuggguu gacgucgucc uagagcacgg cggcugcgua      660

accguaaugg cucaagacaa acccaccguc gacaucgagc uagucacaac caccguaagc      720

aacauggccg aaguucgaag cuacugcuac gaagcaucca uuagcgacau ggcaucagac      780

ucaaggugcc caacacaagg agaggcuuac uuagacaaac aaagcgacac gcaauacgug      840

ugcaaacgca cccuagucga ccgcggaugg ggcaacggcu gcggccucuu cgguaagggc      900

agccugguaa ccugcgcaaa auucgccugc ucaaagaaaa ugacaggaaa aagcauacag      960

cccgaaaauc ucgaauaccg uauaaugcua agcguccacg gaucacaaca cagcggaaug     1020

aucgucaacg acaccggaca cgaaacagau gaaaaccgag ccaaagucga aaucacccca     1080

aacagccccc gcgcugaagc cacccugggc ggguucggau cacuaggccu agacugcgag     1140

ccgcgcacag gccuggacuu cagcgaccuc uacuaccuaa ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacauc ccacucccau ggcacgccgg cgcagacacc     1260

ggaaccccgc acuggaacaa caaagaagca cucgucgagu ucaaagacgc ccacgccaaa     1320

cgccagacag ucgucguccu gggaucccaa gaaggcgccg uccacacagc acucgccggc     1380

gcccuagagg ccgaaaugga cggagcaaaa ggacgccuau caaguggcca ucucaaaugc     1440

cggcuuaaaa uggacaaacu uagauugaaa ggcgucuccu acucucucug uaccgcggcc     1500

uucacauuca caaaaauacc cgcggaaaca cuccacggca cagucaccgu cgagguccag     1560

uacgcaggga ccgaugggcc gugcaaagua ccagcccaaa uggccguaga caugcaaaca     1620

cuaacccccg ucggacgucu aauaacagcc aaccccguca uaacggaauc aacagagaac     1680

uccaaaauga uguuagaauu agacccucca uucggcgacu cguacaucgu cauaggggua     1740

ggagaaaaga aaaucacaca ccacuggcac cgcagcggaa gcaccauagg caaagcguuc     1800

gaagccaccg uacgaggcgc caaacgcaug gccguccucg gagauaccgc cugggacuuc     1860

ggaagcgucg gcggagcccu caacucucua ggaaaaggca uccaccaaau auucggagcc     1920

gcauucaaaa gccuauucgg aggcauguca ugguucuccc aaauacucau cggaacgcuc     1980

uuaauguggc uaggccucaa uaccaaaaac ggcuccauau cccuagucug cuuagcccuc     2040

ggcggcgucc uaauauuccu aagcaccgca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5163
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 1 opt

<400>  5163
gggagaaagc uuaccaugac aucaguaggg aucgucgguc ugcuccugac cacagcaaug       60

gcaguggaag ucacacgacg cggcagcgcg uacuacaugu accucgaccg aucagacgca      120

ggcgaagcaa ucuccuuccc caccacacuc ggcaugaaca aaugcuacau ccaaaucaug      180

gaccuaggac acaugugcga cgcaacaaug ucauacgaau gcccaaugcu ggacgaagga      240

guagaacccg acgacgucga cugcuggugc aacacuacca gcacaugggu cguauacggg      300

acuugccauc acaagaaagg ggaagcaaga cgcucacgac gagcggucac gcucccgagc      360

cacucaacac ggaaacucca aacucggucg caaaccuggc uagaaucaag agaguacaca      420

aaacaccuaa uacgaguaga aaacuggauc uucagaaacc caggcuucgc ccucgccgcg      480

gccgccaucg ccuggcuccu aggcagcagc accucccaaa aagucauaua ccugguaaug      540

auccuacuga uagcaccagc cuacucaauc aggugcauag gaguaagcaa ccgagauuuc      600

guagaaggaa uguccggagg cacaugggua gacguggucc uggaacacgg cggaugcgug      660

acagucaugg cccaagacaa accagccguc gauauagaac ucgucaccac aaccguaagc      720

aacauggcag aaguaagauc cuacugcuac gaagccagca uaagugacau ggcaagcgac      780

agccgcugcc ccacccaagg cgaagccuac cucgacaaac aaucagacac ccaguacgua      840

ugcaaacgca cauuaguaga cagaggcugg ggcaacggcu gcggacuauu cgggaaaggc      900

ucgcugguaa ccugcgccaa auucgcgugc uccaagaaaa ugaccggcaa aagcauacag      960

cccgaaaacc ucgaauaccg gauaauguug uccgugcacg gcagccaaca cuccggcaug     1020

aucgucaacg acaccggaca ugagacagac gaaaaccgcg ccaaaguaga aauaaccccu     1080

aacagcccgc gagccgaggc gacccucgga gguuucggcu cguuaggccu cgauugcgaa     1140

ccccguaccg gacuagacuu cuccgacuua uacuaccuca cgaugaacaa caaacacugg     1200

cuuguacaca aagaaugguu ccacgacaua ccacugcccu ggcacgccgg cgcagacaca     1260

ggcaccccac acuggaacaa caaagaggcc cuaguagaau uuaaagacgc ccaugcaaaa     1320

cgccaaacag uaguaguccu cggcagccaa gaaggcgcag ugcacacagc acuagccgga     1380

gcccuagagg ccgaaaugga cggcgcaaaa gggcgacuaa guagcggaca ccuaaaaugc     1440

cggcucaaaa uggacaaacu ccgccucaaa ggcgucuccu acucccuaug caccgcagca     1500

uuuacauuca caaaaauccc cgcagaaacc cuacauggaa ccgugacagu agaaguccaa     1560

uacgcaggaa cggacgggcc augcaaagug cccgcacaaa uggcaguaga caugcaaaca     1620

cucacaccag ucggcagacu aaucaccgcg aaucccguga uaaccgaauc aaccgaaaac     1680

agcaaaauga ugcuagaacu agacccaccc uucggcgaca guuacauagu cauaggcgua     1740

ggagaaaaga aaaucacaca ccacuggcac cgauccggau caacaauagg aaaagcguuc     1800

gaagcgaccg uacgcggagc caaacgaaug gcaguucucg gagacacagc cugggacuuc     1860

ggaagcguag gcggcgcgcu aaacagccuc ggcaaaggca uccaucagau cuucggagca     1920

gcguucaaau cacuauucgg agggaugagc ugguucaguc agauacuaau aggcacauug     1980

uuaguguggc ucggccuaaa cacgaaaaac ggcagcauca gcuuaaccug ccucgcacuc     2040

ggcggcguac ucauuuuucu aagcaccgca guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5164
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 1 opt

<400>  5164
gggagaaagc uuaccaugac aucagucggc aucguaggcc uccuucuaac aaccgcaaug       60

gccguagagg uaacgcgucg aggcucagcg uacuacaugu accuggaccg aucagacgcg      120

ggggaggcga uaucguuccc aaccacgcuc ggaaugaaca aaugcuacau acaaauaaug      180

gacuuaggac acaugugcga cgccaccaug uccuacgaau gccccaugcu cgacgaagga      240

guggaaccag acgacgucga cugcuggugc aacacaacca guaccugggu agucuacggc      300

acgugccacc acaagaaagg cgaagcccgc cgcucccgcc gagcugucac acucccaagc      360

cauagcacac gcaaacugca gaccagaagc caaaccuggc ucgaaucuag agaauauaca      420

aaacaccuga uccgcguaga gaacuggaua uucagaaacc caggauucgc ccuagccgca      480

gcagucaucg ccuggcuacu cggauccagc acaucacaga agguaauaua ccucguaaug      540

auauuacuca ucgcaccagc guauucgauc cggugcaucg gcguaagcaa ccgagacuuc      600

guugaaggaa ugucaggcgg gaccuggguc gacgucgucc uggaacacgg aggaugcguu      660

acggucaugg cacaagacaa acccacaguc gacaucgaac ucgucacaac gaccguaagu      720

aacauggcag aaguacgguc cuacugcuac gaagcuagca uaucagacau ggcuucagac      780

uccagaugcc ccacacaggg cgaagccuac cuagacaaac aaucagacac gcaauacguc      840

ugcaaacgaa cccucguaga ccgaggcugg ggaaacggcu gcggccucuu cggcaaagga      900

ucgcucguga ccugcgccaa guuugcaugc uccaagaaaa ugacaggcaa auccauacaa      960

cccgagaacc ucgaauaccg caucaugcuc agcguccacg gaucacaaca cucgggaaug     1020

auagucaacg acaccgggca cgagaccgac gaaaaccgcg ccaaggugga aauaacaccu     1080

aacaguccca gagcagaagc cacacucggc ggauucgggu cacucggccu cgacugcgaa     1140

ccacgaaccg gacuagacuu cagcgaccuc uacuaccuga caaugaacaa caaacacugg     1200

cuuguacaca aagaaugguu ccacgauaua ccacucccau ggcacgccgg ggccgauacc     1260

gguaccccac acuggaacaa caaagaggca cucguagagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg ucgucguacu cgggucacaa gaaggcgccg ugcauacagc ccucgcuggc     1380

gcccuagaag ccgagaugga cggcgcuaag ggucgacuuu ccucaggcca ccuaaaaugc     1440

cgccugaaaa uggacaaacu ccggcucaaa ggggucuccu acucacucug caccgcagcc     1500

uucacuuuca ccaagauacc cgccgaaacc cuccacggca ccgucaccgu cgaaguccaa     1560

uacgcaggaa ccgacggacc cugcaaagua cccgcucaaa uggcgguaga caugcaaacc     1620

cugacgcccg ucggccgccu aauuaccgcc aauccuguca uaaccgaauc gacagaaaac     1680

agcaaaauga ugcucgaacu ggacccaccc uucggcgaca gcuacauagu caucggcguc     1740

ggcgaaaaga aaaucaccca ccacuggcac cgauccggua gcaccauagg aaaagcauuc     1800

gaagccaccg uacggggagc aaaaagaaug gcgguacugg gcgacaccgc augggacuuc     1860

gggagugucg gaggagcccu uaacucguua ggcaaaggca uacaccagau auucggcgcc     1920

gccuucaaaa gccuguucgg cggcaugagc ugguucagcc aaauccuaau cgggacacuc     1980

cuggucuggc uaggccucaa caccaagaac ggauccauua gccuaaccug ccuggcacuc     2040

ggaggaguuc uaauauuccu cagcaccgcc gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5165
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 1 opt

<400>  5165
gggagaaagc uuaccaugac gagcguaggc auaguaggcc uccuacuaac caccgcaaug       60

gccguagagg uaacccgccg aggcucagcc uacuacaugu accuagaccg aagcgacgcc      120

ggcgaagcca ucagcuuucc cacgacccua ggagucaaca aaugcuacau acaaauaaug      180

gaccucgggc acaugugcga cgcaaccaug ucauacgagu gcccaaugcu agacgaagga      240

gucgaacccg augacgucga uugcuggugu aacaccaccu caacgugggu cgucuacgga      300

acaugccacc acaagaaagg cgaagcacgg cggagcagac gagcagucac gcuaccaucc      360

cacucaacac gcaaacucca aacccgauca caaacauggc ucgaaucccg cgaauacacc      420

aaacaccuaa ucagaguaga aaacuggauc uuucgcaacc ccgguuucgc acucgcagca      480

gccgcuaucg cauggcuacu aggcucaucc accucacaga aagucauuua ccucgugaug      540

auccuacuaa ucgcacccgc auacucgauc cgcugcauag gaguuagcaa ccgcgacuuc      600

guagaaggaa ugagcggcgg cacaugggua gacguagugc uagaacacgg cggcugcguc      660

accgucaugg cccaggacaa acccacggua gacaucgaau uagucacuac aacggucagc      720

aacauggcag aaguccgcuc auacugcuac gaggcaagca ucuccgacau ggcuuccgac      780

agccgcuguc ccacgcaggg cgaagccuac cuagacaaac aauccgacac acaauacguc      840

ugcaaaagga cccuaguaga cagaggaugg ggaaacgggu gcggacucuu cggaaaaggc      900

agccucguga ccugcgccaa auucgccugc ucaaagaaaa ugaccggaaa aagcauccaa      960

cccgaaaauc ucgaguacag aaucaugcuc agcguccacg gaucacaaca cagcggcaug     1020

auagucaacg acacggggca cgaaacggau gaaaaccgcg ccaaagucga aaucaccccc     1080

aacagccccc gagccgaagc cacccucggc ggguucggau cgcuaggacu cgacugcgaa     1140

ccacgaacag gccuggacuu cagcgaccuc uacuaccuaa cgaugaacaa uaaacacugg     1200

cuaguacaca aagaaugguu ucacgacauc ccacuccccu ggcacgccgg agccgacacc     1260

ggaacacccc acuggaacaa caaagaagca cugguugagu ucaaggacgc acacgcaaaa     1320

cgccagaccg ugguugugcu aggcucacaa gaaggcgcag uacacaccgc ccuggcagga     1380

gcucuagaag ccgagaugga cggagccaaa gggcgccuca gcuccggaca ccucaaaugc     1440

cgccuaaaaa uggacaaacu ccgacuaaaa ggagucucuu acucacucug caccgccgca     1500

uucaccuuca ccaaaauacc cgcagagacc cuccacggca cagugaccgu cgaaguacaa     1560

uacgccggaa cagacggccc augcaaagua ccggcccaaa uggccguaga caugcaaaca     1620

cucaccccag ucggcagacu aaucacagca aaccccguca uaacagaauc cacagagaac     1680

agcaaaauga ugcucgaacu agauccgccc uucggcgaua gcuacauagu aaucgguguc     1740

ggagacaaga agaucaccca ccacugguac cgauccggua gcaccaucgg caaagcauuc     1800

gaagccaccg uccgaggcgc caaaagaaug gcaguccucg gcgacaccgc gugggacuuc     1860

ggcagcguag gaggagcccu caacucccuc ggaaaaggca uccaccaaau cuucggcgcc     1920

gccuucaaaa gccuauucgg uggaaugagc ugguucuccc agauccugau uggcacgcuu     1980

cuaguauggc uaggccuaaa cacgaagaac ggcagcauau cccucacgug ccuugcgcua     2040

ggcggcguac uaauauucuu aagcaccgcc guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5166
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 1 opt

<400>  5166
gggagaaagc uuaccaugac cagcgucggc aucgugggcc uccuccuaac uaccgccaug       60

gccgucgaag ucacacgccg cggcagcgcg uauuacaugu accuggacag aagcgaugcc      120

ggaaaagcca uaucauuccc caccacccua ggaguuaaua aaugcuacau acaaaucaug      180

gaccucggcc acaugugcga cgcaaccaug uccuacgaau gcccaaugcu agacgaaggc      240

guagaaccug acgacgugga cugcuggugc aacaccacau caaccugggu cguuuacggc      300

acaugccacc acaagaaagg ggaagcccga cgcuccaggc gcgcggucac ccucccauca      360

cacucaaccc gcaaacuaca aacacgaucc cagacuuggc uagaaucgcg cgaauacacc      420

aaacaccuca ucagagucga gaacuggauc uuccgaaacc ccggcuucgc acuagcagcc      480

gcugccauag caugguuauu aggcucgagc accucacaga aagugauaua ucucgucaug      540

auacugcuca ucgcgccagc guacuccauu cggugcauag gcguaagcaa cagagacuuc      600

gucgaaggca uguccggcgg aaccugggua gacgucgucc ucgaacacgg uggcugcgug      660

accguaaugg cccaagacaa acccacagua gacaucgaac ugguuaccac aaccguaucc      720

aacauggccg aagugcgcuc auauugcuau gaagccagca ucucagacau ggcaucggac      780

ucccgcugcc caacacaagg agaagcguac cucgacaaac aaagcgacac ccaguacguc      840

ugcaaaagga cacucgucga cagaggcugg ggcaacggau gcggccucuu cggcaaaggc      900

agccucguaa ccugcgccaa auuugccugu agcaagaaaa ugaccggcaa aagcauacaa      960

cccgaaaacc ucgaauaccg gauuaugcuc agcguccacg gcucacaaca cagcggcaug     1020

aucgucaaug acauaggcca cgagaccgac gaaaaccgcg ccaaagucga gaucaccccc     1080

aacuccccac gcgcagaggc aacucucggg ggauucggcu cccucggcuu agacugcgaa     1140

ccccgaaccg gacucgacuu cucagaccug uacuaccuca ccaugaauaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacaua ccccuccccu ggcacgcagg cgcggacacc     1260

ggaacaccgc acuggaacaa caaagaggcc cucgucgagu ucaaagacgc acacgccaaa     1320

cgucaaacag uagucguacu cggcucccaa gaaggcgccg uacauaccgc acucgccggc     1380

gcccuagaag cagaaaugga cggcgccaaa ggacgucuaa gcuccggcca ccucaaaugc     1440

agacugaaaa uggauaaacu acgccucaaa ggcgucagcu acucccucug cacagccgcu     1500

uucacauuua ccaaaauccc agccgaaaca uuacacggaa cuguuaccgu agaaguccag     1560

uacgccggca ccgacggccc augcaaaguc ccagcccaaa uggcagucga uaugcaaaca     1620

cuuacaccag ucggacgccu caucacagca aacccgguaa uaaccgaauc aaccgaaaac     1680

uccaaaauga ugcuagagcu cgaucccccu uuuggcgaca gcuacaucgu caucggaguc     1740

ggggacaaga aaaucaccca ccacuggcac cgcucaggcu ccaccaucgg gaaagcauuc     1800

gaagccacag uacguggugc caaacgcaug gccguacucg gcgacacagc gugggacuuc     1860

ggcagcgucg gaggcgcacu caacucacuc gggaaaggca uccaccaaau cuucggcgcc     1920

gcauucaaau cccuauucgg cggcauguca ugguucuccc aaauacuaau cggcacccua     1980

cucgucuggc uaggacucaa caccaaaaac ggaagcauca gccucacgug ccuagcccuc     2040

ggaggcgucc ucauauuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5167
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 1 opt

<400>  5167
gggagaaagc uuaccaugac aagcaucggc aucgucgggc uccuacucac caccgcaaug       60

gccgcagaaa ucacccgaag aggaagcgcc uacuacaugu accuggaccg gagcgacgca      120

ggaaaagcca uuagcuucgc caccacacua ggaguaaaca aaugucacgu gcagaucaug      180

gaccuaggcc acauguguga cgcgacaaug uccuacgaau gcccaaugcu ggaugaagga      240

guagaacccg acgacgugga cugcuggugc aacacaacua gcacaugggu cgucuacggc      300

acaugccacc acaagaaagg agaggcccga agaucccggc gcgcagucac ccuaccgucc      360

cacaguaccc gcaaacucca aaccagaagc caaacauggc uagaaagcag agaauacacg      420

aaacaccuca ucaaaguuga aaacuggauc uuccgaaacc ccggauucac gcugguagca      480

gucgcaaucg cauggcuccu agggucuagc acuucacaaa aaguaauaua ccucgucaug      540

auccuucuga uagcgccggc cuacagcaua cgcugcaucg gaguauccaa uagagacuuc      600

guagagggga uguccggcgg cacauggguc gacguagucc ucgaacaugg aggcuguguc      660

accguaaugg cccaggacaa gccaaccgua gacaucgagc ucguaacaac caccguaucc      720

aacauggcgg aaguaagaag cuacugcuac gaagccagca ucagcgacau ggccuccgac      780

ucgcggugcc caacccaagg cgaagccuau cucgacaagc aauccgacac acaguacgua      840

ugcaaaagaa cccucgucga ucgcggcugg ggcaacgggu gcggccucuu cggaaaaggc      900

agccucguaa ccugcgcaaa auuuaccugc uccaaaaaga ugaccggaaa aucaauccag      960

ccagaaaacc ucgaauaccg caucaugcuc agcguccacg gaagccaaca uagcggcaug     1020

aucguaaacg acacaggaca cgaaaccgac gaaaaccgcg caaagguaga aguaaccccc     1080

aacagcccac gcgcggaagc gacgcuagga ggcuucggaa gccugggacu cgacugcgaa     1140

ccccgcacgg gccuggacuu cucagaccuc uacuaccuca caaugaacaa caagcacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccacucccgu ggcacgcggg cgcagacacc     1260

ggaacacccc acuggaacaa caaagaggcc cuaguggagu ucaaagacgc acacgccaaa     1320

cgacaaaccg uagucguccu aggcagccaa gaaggcgccg uccacaccgc ccucgcaggc     1380

gcccuagaag ccgaaaugga cggagcaaaa ggacgacuau ucucgggaca ccucaaaugc     1440

agacucaaaa uggacaaacu uagacuaaaa ggggucucau acagccucug uacagcagca     1500

uucaccuuca cgaaaguacc ggccgaaaca cuacacggaa ccguaacagu agagguacaa     1560

uacgcaggga cggacggccc gugcaaaguc ccagcccaaa uggcuguaga caugcaaacc     1620

cuaacaccag ucggccgccu cauaaccgca aacccaguua ucacagaauc aacagaaaac     1680

ucaaaaauga ugcuagaacu agacccaccc uucggagaca gcuacauagu cauaggaguu     1740

ggagacaaga aaauaacaca ccacuggcac cggucaggga gcacaaucgg aaaagccuuc     1800

gaagcaaccg uaagaggcgc aaaaagaaug gcgguccuug gcgacaccgc augggacuuc     1860

ggaucaguug gaggcguguu caacucacuc gggaaaggca uacaccaaau auucggagcg     1920

gcauucaaau cacuauucgg gggaaugucc ugguucaguc aaauacucau aggcacccug     1980

cuaguauggc uugggcuaaa cacaaagaac ggauccauaa gccuaaccug ccucgcacug     2040

ggaggcguca ugaucuuccu aucaaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5168
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 1 opt

<400>  5168
gggagaaagc uuaccaugac aucaauaggg aucauaggcc uccuccugac aaccgcaaug       60

gcggcugaga uaacgcgccg agggagcgcc uacuacaugu accuagacag aucggacgca      120

gggaaagcaa ucagcuucgc caccaacuug ggagucaaca aaugccacgu ccaaaucaug      180

gaccucggac acaugugcga cgccacaaug ucuuacgagu gccccaugcu agacgaaggg      240

guagaacccg acgacgugga cuguuggugc aacaccacaa gcaccugggu agucuacgga      300

accugccacc acaagaaagg cgaagcccgg agaucccgcc gagccguaac ccuccccucc      360

cacucgaccc gaaaacucca gacaagaagu caaaccuggc uugaauccag agaauacacg      420

aaacaccuaa uaaaaguuga aaacuggauc uuccgcaacc ccggcuucgc ccucgccgcg      480

gucgccauag ccuggcuccu aggcagcucg accagucaaa aaguaaucua ccuagucaug      540

auccuacuca ucgccccggc auacagcaua cgauguaucg gagugagcaa ccgagacuuc      600

gucgaaggaa ugagcggcgg aacuugggua gacguugucu uagagcacgg cggcugcgua      660

accgucaugg cccaggacaa accaaccguc gacaucgagc uagugaccac cacggucuca      720

aacauggccg aagugagauc guacugcuac gaagccucca uauccgacau ggcaucagac      780

ucgagaugcc caacacaagg cgaagccuac cucgacaaac aaagcgacac acaauacgug      840

ugcaaacgca cccuaguaga cagaggaugg ggcaacggau gcggacucuu cggaaaaggc      900

agccuaguca cuugcgcgaa auucacaugc agcaagaaaa ugaccggcaa aagcauacaa      960

ccggaaaacc uagaauaccg caucaugcuc agcguacacg guucacaaca cuccggcaug     1020

aucgucaacg acauaggaca cgaaaccgac gaaaaccgcg caaaagugga agucacccca     1080

aacucaccga gagcagaagc aacacucggc ggauucggau cacuaggucu cgacugcgaa     1140

ccgcgcacug gccuagacuu cuccgaccuc uacuaccuua caaugaacaa caagcacugg     1200

cuaguccaca aagaaugguu ccaugacauu ccguugccau ggcacgcagg agccgacaca     1260

ggaacaccac acuggaacaa caaagaagcg cuaguagagu ucaaagacgc ccacgccaag     1320

cgucaaaccg ugguaguccu aggaagccaa gaaggcgccg ugcacacagc acuagccgga     1380

gcacucgaag ccgaaaugga cggugccaaa gggcgacuau ucagcggcca ccucaaaugc     1440

aggcucaaaa uggacaaacu gcgacuaaaa ggaguaucgu acucacuaug caccgccgcc     1500

uucacauuca caaaaguacc ggccgaaacc cuacacggca caguaaccgu ggaaguccag     1560

uacgcaggaa ccgacggacc uugcaaaguc cccgcccaaa uggccgucga caugcaaaca     1620

cucacacccg ucggcagacu gauaaccgca aacccaguaa ucaccgaaag cacugagaac     1680

agcaaaauga uguuagaacu agauccccca uucggcgacu cauacaucgu caucggaguc     1740

ggcgacaaga aaauaaccca ccacuggcac cgcaguggcu caaccaucgg caaagcauuc     1800

gaagccaccg uacgcggcgc caaacgcaug gcaguccucg gugacacagc augggauuuc     1860

ggaagcgucg gcggcgucuu caacucccuc ggcaaaggug ugcaccaaau cuucggcgcc     1920

gccuucaaga gccucuucgg aggcaugucg ugguucucac aaauccuuau cgggacacuc     1980

cuagucuggc uaggacuaaa caccaagaac ggcaguauca gccuaaccug ccucgcccua     2040

ggcgggguaa ugauauuccu aagcacggcc guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5169
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 1 opt

<400>  5169
gggagaaagc uuaccaugac cuccaucggg aucauaggcc uauuacuaac caccgcaaug       60

gcagccgaga ucacccggcg cgguagcgcc uacuacaugu accucgauag aucagaugca      120

ggcaaagcaa uauccuucgc aaccacacuc ggaguaaaca agugccacgu ccaaaucaug      180

gaccuaggcc acaugugcga cgcaacaaug ucauacgaau gcccaaugcu agacgagggc      240

gucgaacccg acgacguaga cugcuggugc aacaccacaa gcacaugggu agucuacgga      300

acaugucacc acaagaaagg agaggccagg agaagccgac gcgcagucac ccucccaagc      360

cacucgacac gaaaacucca aacacgcagc caaacauggc uggaaagccg cgaauacacc      420

aaacaccuca ucaaaguaga aaacuggauc uuccgcaacc caggcuucgc acucguagcc      480

guagccauag ccuggcuccu gggaagcagc accagccaaa aagucauaua ccucguaaug      540

auccuccuga ucgcaccagc cuacucgauc aggugcauug gcgucagcaa ccgagacuuc      600

gucgaaggca uguccggagg cacaugggua gacgucgugc uagagcacgg cggcugcguc      660

acggucaugg cccaagacaa acccacggug gacauugaac ucguaaccac cacagucagc      720

aacauggcug aagucagaag cuacugcuac gaggcaucga ucagcgacau ggccagugac      780

ucaagaugcc caacacaagg ggaagcauac cucgacaaac agucagacac ccaauacguc      840

ugcaagcgaa cacucguaga ccgcggcugg ggcaacggcu gcggacucuu cggcaagggc      900

ucucugguca caugcgccaa auucacgugu agcaagaaaa ugacuggcaa aucaauccaa      960

ccagaaaauc ucgaauaccg aaucaugcua uccguacacg gcucacaaca cagcggcaug     1020

auaguaaaug acauagguca ugaaacugac gaaaacagag caaaagucga gguaaccccc     1080

aacagcccgc gcgcugaagc aacgcucggc ggcuucggga gccuaggacu cgacugcgaa     1140

ccccgaacgg gccuagacuu uagcgaucug uacuaccuca caaugaacaa caaacacugg     1200

cucgugcaca aagagugguu ccacgacauc ccccuccccu ggcacgccgg agccgacacc     1260

ggcacacccc auuggaacaa caaagaagcc cucguggagu ucaaggacgc acacgcaaaa     1320

cggcaaaccg uagucguccu aggaucacag gaaggcgccg uccacaccgc cuuagcggga     1380

gcacucgaag cagaaaugga cggcgccaaa gggcgccuau ucuccggaca ccugaagugu     1440

cgucucaaaa uggacaaacu ccgacuaaaa ggaguguccu acagccucug caccgcagca     1500

uucacauuca ccaaaguccc cgccgaaacc cuccacggga caguaacagu cgaaguccaa     1560

uacgccggua cagacggacc augcaaaguc cccgcccaaa uggcaguaga caugcaaaca     1620

cuaacccccg ucgggcgacu caucaccgcc aaccccguua uaacagaaag cacggaaaac     1680

agcaaaauga ugcuggaacu ugacccuccc uucggggacu cauacauagu aauaggcguc     1740

ggagacaaga aaaucacaca ccauuggcac cgaagcggau ccacaauagg aaaagcauuc     1800

gaagccaccg uacgaggggc aaaacggaug gcaguacuag gcgacaccgc cugggacuuu     1860

ggcucaguag gcggcgucuu caacagccuc gggaaaggca uccaccaaau auucggugcc     1920

gccuucaaau cacucuucgg cggaaugagc ugguucagcc aaauccugau uggaacccua     1980

cucgucuggc ugggcuuaaa caccaaaaac ggcagcaucu cccugacaug ucucgcccua     2040

gggggaguaa ugauauuccu aucaaccgcc gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5170
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 1 opt

<400>  5170
gggagaaagc uuaccaugac aagcauaggu aucauagggc uucuacucac aaccgccaug       60

gcagccgaaa ucacccgacg gggcuccgcc uacuacaugu acuuagacag aagcgacgca      120

ggaaaagcca ucucauucgc caccaaccua ggagucaaca agugccacgu acaaauaaug      180

gaccugggac acaugugcga cgccacuaug uccuacgaau gccccaugcu cgacgagggc      240

gucgaaccag acgacguuga cugcuggugc aacaccacaa gcacaugggu cgucuacggg      300

accugccacc acaagaaagg cgaagcacgc cggagccggc gcgccgucac gcuuccgagc      360

cacagcaccc gcaaacuaca aacaagauca cagacguggc ucgagagcag agaauacacc      420

aagcaccuca ucaaaguaga aaacuggauu uuccgcaacc ccggauucgc ucuagccgca      480

gucgcaaucg ccuggcuccu aggcucgagc acaagccaaa aagucaucua ccuaguaaug      540

auccuccuaa ucgcacccgc auacucaaua cgcugcauag gcgucagcaa ccgcgacuuu      600

guagaaggaa ugucaggagg gacaugggua gacguagucc ucgaacacgg aggcugcguc      660

accgucaugg cacaggacaa acccaccgua gacauagagc ucguaaccac aacaguaagc      720

aauauggcgg aaguccguuc cuacuguuac gaggcuucaa ucucagacau ggcgucagac      780

ucacgauguc ccacccaagg cgaagcauac cuggacaaac aaucagacac ccaauacgua      840

uguaaaagga cccucguuga ccgcggcugg gggaacggau gcgggcuauu cggaaaagga      900

ucccucguca ccugugccaa auucacaugc ucaaagaaaa ugaccggaaa aucaauccag      960

ccggaaaacc uagaauacag gauaaugcuc ucaguccacg gaagccaaca cagcggaaug     1020

auagucaacg acgaaaacag ggccaaaguc gagguuacac ccaacagccc acgcgccgaa     1080

gcaacacuag gaggauucgg gagccuaggu cuugacugcg aaccgcgaac cggccuggac     1140

uucucagacc ucuacuaccu aaccaugaac aacaaacacu ggcucguaca caaagaaugg     1200

uuccacgaca ucccgcuccc guggcacgcc ggagcagaca caggcacccc acacuggaac     1260

aacaaagagg cccucguaga guucaaagac gcccacgcga aacgccaaac aguaguagua     1320

cuggguuccc aagaaggcgc aguccacacc gcgcuagcag gcgcacuaga agccgaaaug     1380

gacggagcca aaggacgucu auucuccgga caccucaaau gccggcuaaa aauggacaaa     1440

cuaagacuca aaggaguauc guacucccuc ugcacagccg ccuuuacauu cacgaagguc     1500

ccagccgaaa cccuacacgg aaccgugacc gucgaaguac aauaugcggg aaccgacggc     1560

ccaugcaagg uaccagccca aauggcaguc gacaugcaaa cccucacgcc cgucggacgc     1620

cucaucacag caaauccagu aaucacagaa agcaccgaaa auucaaaaau gaugcuggaa     1680

cuagacccac ccuucggaga cagcuacauc guaaucggcg ucggagacaa gaaaaucaca     1740

caucacuggc auaggagcgg gagcacaauu ggaaaagccu ucgaagcaac cguaagaggc     1800

gccaaacgaa uggcgguccu aggugacaca gccugggacu ucggcagcgu cgggggcguc     1860

uucaacagcc uaggcaaagg cauccaccaa auauucggag cagccuuuaa aagccuauuc     1920

gguggcaugu caugguucag ccagauccuc auaggaaccc ugcuagucug gcuaggacua     1980

aacacgaaaa acgggagcau aagccugaca ugccucgcuc ugggaggcgu caugauauuc     2040

uuaagcacgg ccguaagcgc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  5171
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 1 opt

<400>  5171
gggagaaagc uuaccaugac cagcauagga auaguaggcc uccuacucac aaccgccaug       60

gcggcggaga ucaccagacg aggcagcgca uacuacaugu accucgacag gucagacgcc      120

gggaaagcaa ucagcuucgc aaccacacug ggagucaaca aaugccacgu acagauaaug      180

gaccugggac acauguguga cgcaaccaug ucauacgagu gccccaugcu agaugaagga      240

guagagcccg acgacguaga cugcuggugc aauacaacca gcacaugggu cguguacgga      300

accugccacc acaagaaagg cgaagcuaga agaagccgca gagcgguaac ccuaccaagc      360

cacagcacgc gaaaacucca aacccgaagc caaaccuggc uugagagccg ggaauacacg      420

aaacaccuaa ucaagguaga aaacuggaua uuccgcaauc ccgguuucgc gcucgucgca      480

gucgcaauag cauggcuccu aggaagcagc acaagccaga aagugauaua ccuaguaaug      540

auccuacuga uagccccagc guacagcauc cgcugcaucg gcguaagcaa ccgcgacuuc      600

guagaaggaa uguccggagg cacaugggua gacguagucc ucgagcacgg cggcugugua      660

acaguaaugg cacaagacaa accaacgguc gacauagagc uagucaccac aacaguaagc      720

aacauggcag aagugcgaag cuacugcuac gaagcaucca ucucagacau ggccagcgac      780

agucgaugcc ccacacaagg cgaagcauac cuagacaaac aauccgacac ccaauacguc      840

ugcaaaagaa cgcuagucga uagagguugg ggcaacggcu gcggauuauu cggcaaaggc      900

ucgcuaguga caugcgcgaa auucacaugc agcaagaaaa ugacgggcaa aagcauccag      960

cccgaaaacc ucgaguacag aauaaugcua uccguccacg gcagccaaca cagcggcaug     1020

auagucaacg acacugggca cgaaacagac gaaaacaggg ccaaaguaga agucacccca     1080

aacagcccac gcgcggaagc gacccucgga ggguucgggu cacucggacu agacugcgaa     1140

ccacgaacag ggcucgacuu cuccgaccuc uacuaccuaa cgaugaacaa caaacacugg     1200

cugguacaca aagagugguu ccacgacaua cccuugccau ggcacgcagg ggcggacaca     1260

ggaaccccac acuggaacaa uaaagaggcg cuagucgagu ucaaagacgc acacgcaaaa     1320

cgacaaacag uaguaguacu aggaagccaa gaaggcgcag uacacacagc acuagccggc     1380

gcgcuggaag ccgaaaugga cggcgcaaaa ggacgacuau ucagcggcca ccugaaaugc     1440

aggcucaaaa uggacaaacu gagacugaaa ggagucuccu acucacuaug cacagcagca     1500

uuuaccuuca cgaaaguacc agccgaaacc cuacacggaa caguaacggu agaaguccag     1560

uacgcaggaa cagacgggcc augcaaagua ccagcacaga uggccgucga caugcagacc     1620

cucacaccag ucgggcgccu aauuacagcc aacccgguaa ucacagaaag caccgaaaac     1680

uccaagauga ugcuagaacu agacccaccc uucggcgaca gcuacaucgu gaucggugua     1740

ggugacaaga aaaucacaca ccacuggcac cggagcggca gcacaauagg gaaagccuuc     1800

gaagcgacag uacgcggcgc gaaacgcaug gccguacucg gcgacaccgc augggacuuc     1860

ggaagcgugg gcggcgucuu caacagccuc ggaaaaggua uccaccaaau cuucggggca     1920

gccuucaaaa gccucuucgg gggaaugagc ugguucucac aaauacuuau agggacacug     1980

cuaguauggc ucggccucaa caccaaaaac ggaagcauaa gccuaaccug ccuagcacug     2040

ggaggcguga ugauauucuu aagcacagcc gucucggcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5172
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 1 opt

<400>  5172
gggagaaagc uuaccaugac gagcauagga aucgucggac uacuacuaac caccgcaaug       60

gccgcagaaa ucacacgcag aggaagcgca uacuacaugu accuagauag gagcgaugcc      120

ggcaaagcca ucagcuucgc gacaacacua ggugucaaca aaugccacgu acaaauaaug      180

gaccuaggcc auaugugcga cgccaccaug ucauacgaau gccccaugcu agacgaaggc      240

gucgaaccag acgacgugga cugcuggugu aauaccacau ccaccugggu agucuacggc      300

acaugccagc acaagaaagg agaggcacga cgcucaagac gagcgguaac acuacccagc      360

cacucaaccc gcaaacugca aacgcgcuca caaacauggc uagaaucaag ggaauacaca      420

aaacaccuaa uaaaagucga aaacuggaua uuccgcaacc ccggcuucgc acuggucgca      480

guagcaauag cguggcuccu aggcucgagu accucccaaa aagucauaua ccucgucaug      540

auccuccuca uagccccagc auacuccauc cgcugcaucg gcgucagcaa cagagacuuc      600

guagaaggca ugucuggagg cacaugggug gacguggugc uagaacacgg aggcugcguc      660

accguaaugg cccaagacaa acccacaguc gacaucgaac ucgucacaac cacgguuagc      720

aacauggcag agguacgcuc cuacugcuau gaagccagca uaucagacau ggcaagcgac      780

ucgcgcugcc ccacccaagg cgaagccuac cucgacaaac aaagugacac gcaauacgua      840

ugcaaaagaa cccuagucga ccgaggcugg ggaaacgguu gcggccucuu cggaaaaggc      900

agccuaguaa caugcgccaa auucacaugc uccaagaaaa ugacagggaa aagcauacaa      960

cccgaaaacc uggaauacag aaucaugcuu agcguacacg gcagccaaca uucagguaug     1020

auaguaaacg acaucggcca cgaaaccgau gagaaccgcg ccaaaguaga agucaccccc     1080

aauuccccgc gcgccgaagc aacccuaggc ggauucggau cacucggccu ugacugcgaa     1140

ccccgcacag gacucgacuu cagcgaccuc uacuaccuaa caaugaacaa caaacacugg     1200

cucguacaca aagagugguu ucacgacauc ccccuacccu ggcacgccgg agccgacacc     1260

ggaacacccc acuggaacaa caaagaagcc cuaguggagu ucaaagacgc acacgccaaa     1320

cgccaaaccg uagucguccu aggcagccaa gaaggcgccg uccacacugc guuagcagga     1380

gcauuggaag ccgaaaugga cggggccaaa ggucggcuau ucagcggcca ccugaaaugc     1440

cgccucaaaa uggacaaacu ucgccuaaaa ggcgucuccu auucacucug caccgccgua     1500

ugcacagcag ccaaaguacc ggcggaaacc cuacacggca ccguaacggu cgaaguacaa     1560

uacgcaggaa cagacggucc augcaaagua cccgcucaaa uggccguaga caugcaaacc     1620

cucacccccg uaggcagacu aaucaccgcc aacccaguaa uaaccgaaag cacggaaaac     1680

uccaaaauga ugcuagaacu agacccgccc uucggcgaca gcuacaucgu cauuggagua     1740

ggggacaaga aaaucacaca ccacuggcac agauccggau ccacaauagg caaagcguuc     1800

gaagcaaccg uccgcggcgc aaaacgcaug gccguccucg gcgacacggc gugggacuuc     1860

gguucaguag gcggagucuu caacucccua ggcaaaggaa uacaccaaau cuucggcgcc     1920

gcauucaaau cgcuguucgg cggcaugucg ugguucucac aaauacucau cgggacccuc     1980

cuaguauggc ugggacucaa uaccaaaaac ggaucaaucu cacucacaug ccuggcgcug     2040

ggcggcguaa ugauauuccu aucaacagca gucucggccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5173
<211>  2266
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 1 opt

<400>  5173
gggagaaagc uuaccaugac uagcaucggc aucgucggcc uccuacugac cacagcuaug       60

gcagcagaaa ucacccgacg gggguccgca uacuacaugu accuagaccg cucagacgcc      120

ggaaaagcga ucuccuucgc aacaacgcua ggagucaaca aaugccacgu ccaaaucaug      180

gaccuaggcc acaugugcga cgcuacaaug agcuacgaau gccccaugcu agaugagggc      240

guagaacccg acgacgucga cugcuggugc aacaccacua gcacgugggu aguauauggc      300

acaugccacc acaaaaaggg agaagcacgg cggagccgac gcgcaguaac acucccaagc      360

cacagcacac gaaaacucca aacccguagc caaacguggc ucgaauccag agaauacacc      420

aaacaccuaa ucaaaguaga aaacuggauc uuccgaaacc caggcuucgc ccucguagca      480

gucgcaauag cauggcuccu aggauccagu acaagccaga aaguaaucua ccuaguaaug      540

auccuccuaa uagcacccgc cuacuccauc agaugcauag gcguaagcaa ccgagacuuu      600

guggaaggaa uguccggcgg aacaugggua gacgucgugc uggaacacgg aggcugcgua      660

acgguaaugg cgcaagacaa acccaccgua gacaucgaac uggucaccac caccguauca      720

aacauggcag aaguacgcuc cuacugcuac gaagcgucaa uaucagacau ggccucggac      780

ucccggugcc caacccaagg cgaggcauac cuagacaaac aauccgacac ccaauacguc      840

ugcaaacgaa cccugguuga ucgaggaugg gguaacggcu gcggacucuu cgguaaaggc      900

agucuaguca ccugugcaaa auucacaugc ucaaagaaaa ugaccggaaa aucaauucaa      960

ccagaaaacc ucgaauaucg aauaaugcuc ucaguacacg gaagccaaca cucaggcaug     1020

auagucaaug acgaaaaccg cgccaaaguu gaagucaccc caaacagucc cagagcagaa     1080

gccacgcucg gcggguucgg gagccuagga cuggacugug aaccccggac gggccuggac     1140

uucagcgacc uauacuaccu caccaugaac aacaaacacu ggcuaguaca caaagaaugg     1200

uuucacgaua uaccccuccc guggcacgcc ggcgccgaua ccggcacucc ccacuggaac     1260

aacaaagaag cacuggucga guucaaagac gcacacgcaa aacgccaaac ugucguaguc     1320

cucggguccc aagaaggcgc cguacacaca gcacucgccg gcgcgcucga ggcagaaaug     1380

gacggcgcca aagguagacu cuucucagga caccuaaagu gccgccuuaa aauggacaaa     1440

cuccgccuca aaggaguguc guacuccuua ugcaccgccg cauucaccuu cacaaaagua     1500

ccagccgaga cacuccacgg caccguaacc guagaaguac aauacgccgg gaccgacgga     1560

ccaugcaagg uacccgcaca aauggccgua gacaugcaaa cacucacgcc agucggacgc     1620

cucaucaccg caaaucccgu caucacagag uccaccgaga acuccaaaau gaugcucgaa     1680

cucgaucccc cauucggcga cuccuacaua gucaucggcg ucggagacaa gaaaaucacc     1740

caccacuggc accgauccgg cucgaccauc ggcaaagccu ucgaagccac cguaagaggc     1800

gccaaacgga uggccguccu gggcgacacg gccugggacu ucggauccgu cgguggcguu     1860

uucaacucac ucggaaaagg aauccaccaa aucuucggag ccgccuucaa aucacuauuc     1920

ggaggcaugu ccugguucag ccaaauccuc aucggaaccc uacucgucug gcuuggucuc     1980

aacaccaaaa acggcagcau cagcuuaaca ugccucgcac ucggaggcgu aaugaucuuu     2040

cucuccaccg ccgucagcgc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2220

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2266


<210>  5174
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 1 opt

<400>  5174
gggagaaagc uuaccaugac aagcauuggc auuauaggcc uacuucucac cacugccaug       60

gccgccgaaa uaacacgacg aggaagcgcc uacuacaugu accuagacag aucggacgca      120

ggcaaagcaa ucagcuucgc aacaacccuc ggagucaaca aaugccacgu acaaaucaug      180

gaccuaggac auaugugcga cgcaacaaug ucauacgaau gccccaugcu ggacgaaggc      240

gucgaacccg acgacguaga cugcuggugc aacaccacaa guaccugggu agucuacgga      300

accugccacc acaaaaaggg agaaacccga cggucccgca gaucaguauc ccuccgcuac      360

cacuacacaa gaaaacucca aacccgaagc caaaccuggc ucgaaucccg cgaauacaaa      420

aaacaccuaa ucaugguaga aaacuggaua uuccggaacc caggauucgc caucgugagu      480

guagccauca cauggcucau gggcagucug accagccaaa aagucaucua ccucguaaug      540

aucgugcuaa uaguacccgc cuacagcauc ucaugcaucg gcgucucaaa ccgcgaccuc      600

guagaaggaa uguccggagg caccuggguc gacgugguuc ucgaacaugg aggaugcgug      660

acggaaaugg cccaagacaa accaaccguc gacaucgaac ucguaacgau gaccgucagc      720

aacauggcag aagucagauc cuacugcuau gaagcguccc ucagcgacau ggcaagcgcc      780

agccgaugcc ccacacaggg cgagcccuca cucgacaaac aaucagacac acaaagcguc      840

uguaaaagaa cccucgggga ccgaggcugg ggcaacgggu gcggcauauu cggaaaaggu      900

ucgcuaguaa caugcucaaa auucaccugc ugcaaaaaaa ugcccggcaa aucgauacaa      960

ccagagaacc ucgaauacag aauaaugcua cccguacacg ggagccaaca cagcggcaug     1020

auaguaaacg acauaggaca cgagacagac gagaacagag caaaaguaga agucacgcca     1080

aauuccccgc gagccgaagc aacacucgga ggcuucggaa gccuagggcu cgacugcgaa     1140

ccacgcaccg gccucgacuu cucagaccua uacuaccuga ccaugaacaa caaacacugg     1200

cuuguccaca aagaaugguu ccacgauauc ccucuacccu ggcacgccgg agcagacacc     1260

ggcacacccc acuggaacaa caaagaggcc cugguagagu ucaaagacgc acacgcaaaa     1320

cgucaaaccg ucguggucuu aggcagccaa gaaggcgccg uacacaccgc acucgccggc     1380

gcccucgaag cggagaugga cggggcuaaa ggacgccugu ucucaggcca ucucaaaugc     1440

cgucuaaaaa uggacaaacu aagacuaaaa ggaguaucgu acagccucug caccgccgcc     1500

uucacauuca ccaaaguacc ggcagaaaca cugcacggaa cagugacagu ggaaguccaa     1560

agcgccggaa cagauggacc cugcaaaguc cccgcccaga uggccguaga caugcaaaca     1620

cucacacccg uaggccggcu aaucacagca aacccgguaa ucaccgaguc aacagaaaac     1680

uccaaaauga ugcuggaacu ggauccgcca uucggagacu cauacauagu cauaggugua     1740

ggagacaaga aaauaaccca ccacuggcac cgaagcggca gcaccauagg aaaagccuuc     1800

gaagccacgg uacgcggagc caaaagaaug gccgugcucg gagacaccgc cugggacuuc     1860

ggcucaguag ggggcguauu caacagccua ggaaaaggca uacaccaaau cuucggagca     1920

gcauuuaaaa gccuauucgg aggaaugagc ugguucucac aaauacuaau cggaacccua     1980

cuaguguggc uaggacucaa caccaaaaac ggcagcaucu cacucacgug ccuagcccug     2040

ggaggaguca ugauauuccu cuccacagcc guauccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5175
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 1 opt

<400>  5175
gggagaaagc uuaccaugag cguaggcaua gucggccuac uccugacgac cgcaauggcc       60

gcagaaguga cacggcgggg cagcgccuac uacauguauc uggacagaaa cgacgcgggg      120

gaagccaucu cauucccaac gacccuaggc augaacaaau gcuacauaca aauaauggac      180

cuagggcaca ugugcgacgc aacgaugucc uacgagugcc cgaugcucga cgaaggugua      240

gaaccagacg acgucgauug cuggugcaac accacaucca cauggguagu auacggcacu      300

ugccaccaua agaaagggga agcacgccgc agccgacgcg ccguaacacu cccuagccac      360

uccacgcgca aacuacaaac aagaagccaa accuggcuag aaucuagaga auacacgaaa      420

caccugauaa gaguagaaaa cuggaucuuc cgaaacccgg gauucgcccu cgccgcagcc      480

gccauagcau ggcuccucgg aagcaguacc agucaaaaag uaaucuaccu gguaaugauc      540

cuccuaaucg cacccgcgua cagcaucagg ugcaucggcg ugagcaaccg cgacuucgua      600

gaagggauga gcggcggaac augggucgac gucguccuag agcacggagg augcgucaca      660

guaauggcac aagacaaacc uacgguagac aucgagcucg ucaccaccac cguaaguaac      720

auggccgaag uacggucaua uugcuacgaa gcauccauaa gcgacauggc cagcgacucc      780

cgauguccaa cucaaggaga agcauaccua gacaaacaau cggacacaca auacguaugc      840

aaaagaaccc ucguagacag aggauggggc aacggaugcg gacuauucgg aaaaggcucg      900

cuaguaaccu gcgccaaauu cgcgugcucc aagaaaauga caggaaaaag cauucaacca      960

gaaaaccuag aauacagaau aaugcugucc guacacggaa gccaacauag cggaaugauc     1020

gucaacgaca ccggacacga aacggacgag aacagagcaa aaguagaaau caccccaaac     1080

aguccccgcg cagaggcgac gcuaggaggu uucggaagcc uaggacugga cugcgaaccc     1140

cgcacgggcc ucgacuucag cgaccuguac uaccucacca ugaacaacaa acacuggcua     1200

gugcacaaag aaugguuuca cgacaucccc cuaccguggc acgcaggcgc agacacagga     1260

acaccgcacu ggaacaacaa agaagcacug gucgaguuca aagacgccca cgcaaaaaga     1320

cagacagugg ugguccuggg cagccaagag ggagccguac acacagcgcu cgcgggagca     1380

cuggaagccg agauggacgg cgcaaaagga cgccuguccu caggacaccu caaaugccga     1440

cuaaaaaugg acaaacucag acucaaagga gucagcuacu cacuuuguac agccgccuuc     1500

acauucacaa aaauaccagc cgaaacacua cacggaacag uaaccgucga aguacaguac     1560

gcaggaacag acggaccgug caaagucccc gcgcaaaugg caguagacau gcaaacccug     1620

accccagucg gacgacuaau aaccgcaaac cccguaauca cagaauccac cgaaaauucc     1680

aaaaugaugc ucgagcuaga cccacccuuc ggcgacagcu acauagugau cggcgucggg     1740

gaaaagaaaa uaacccacca cuggcaccga agugggagua caauaggcaa agcauucgag     1800

gcaaccguca gaggggccaa acgaauggca guacugggag acacagcaug ggacuucgga     1860

ucagugggcg gagcccuaaa cucacucgga aaaggaaucc aucaaaucuu cggggccgcg     1920

uucaaaucac ucuucggugg caugagcugg uucagccaaa uacucauagg cacacuccua     1980

auguggcucg gacugaacac gaaaaacgga agcauaagcc ugaugugccu ggcacuaggc     2040

ggcguccuca uauuuuuaag uacagccgua agcgccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  5176
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 1 opt

<400>  5176
gggagaaagc uuaccaugac cuccgugggg auagucggac uacuccugac aaccgccaug       60

gccgccgaag uuacccgacg aggcagcgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcaa ucuccuuccc caccacgcua ggcaugaaca aaugcuacau acaaaucaug      180

gaccucggac acaugugcga cgcaacaaug agcuacgaau gccccaugcu cgacgaggga      240

gucgaacccg acgaugucga cugcuggugc aacacaacca gcaccugggu cguguacgga      300

accugccacc acaaaaaggg cgaagcgcgc cgcucccggc gcgccgucac ccuccccagc      360

cacucaacac gaaaacuaca aacacgaagc caaacauggc uggaaucacg agaauacacc      420

aaacaccuaa uacgagucga aaacuggaua uuccgaaacc ccggcuucgc acuagccgcc      480

gcggccauag ccuggcuccu cggcucauca accucccaaa aaguaaucua ccucguaaug      540

auacuccuaa uagcccccgc auacucgaua agaugcauag gcgucagcaa ccgcgacuuc      600

guagaaggaa ugagcggcgg caccuggguc gacguugucc uagaacacgg cggaugcguc      660

acaguaaugg cccaagacaa accuaccguc gacauagaac ucgucacaac aaccguuucc      720

aacauggccg aaguacgauc auacugcuac gaggcuucaa uauccgacau ggccucggac      780

ucccgcugcc ccacccaagg cgaagccuac cucgacaaac agagcgacac ccaauacguc      840

ugcaaacgca cgcuagucga ccgcggaugg ggcaacggcu gcggacuauu cgggaaagga      900

ucccuaguaa caugugcaaa auucgccugc ucaaagaaaa ugacggguaa aucaauccaa      960

ccggaaaacc ucgaguaucg aaucaugcuc uccguccaug gcucccaaca cuccggcaug     1020

auaguaaaug acacaggcca cgaaaccgau gaaaacagag cuaaaguuga aauaaccccc     1080

aacucaccac gcgccgaagc cacccugggc ggcuuuggau cacugggccu cgacugcgaa     1140

ccccgaaccg gucucgacuu cagcgaccug uacuaccuaa caaugaacaa caaacacugg     1200

cuaguccaca aggagugguu ccacgacauc ccacucccau ggcacgccgg agcagacaca     1260

ggcaccccac acuggaacaa caaggaagcc cuagucgagu ucaaagacgc acacgcaaaa     1320

cgacaaacgg ucgucguccu agggagccaa gaaggagcag uacacacagc acucgcaggg     1380

gcgcuagaag cagaaaugga cggggcaaaa ggacgccuuu ccagugggca cuuaaagugc     1440

agacucaaaa uggacaaacu uagauuaaaa ggcguaucau acucacuaug caccgccgcc     1500

uucaccuuca ccaaaauacc cgccgaaacg cugcacggaa cagucacggu ugaaguccaa     1560

uacgccggca cagacgggcc cugcaaagua cccgcgcaaa uggcggucga caugcagaca     1620

cuuacccccg uuggucggcu aauaacggcc aacccaguca uaacagaaag caccgaaaac     1680

uccaaaauga ugcuagaacu cgacccaccc uucggagaca gcuacaucgu caucggcguu     1740

ggcgaaaaga aaaucacaca ucacuggcac agaucaggua gcaccauagg caaagccuuu     1800

gaagccacag ucagaggcgc caggcguaug gcaguccucg gagacaccgc cugggacuuu     1860

ggauccgugg gaggcgcacu aaacucccua ggcaaaggca uccaucaaau auucggggcc     1920

gccuucaaau cgcuguucgg cggaauguca ugguucagcc aaauccuaau cggaacgcuc     1980

cuuauguggc ucggccuaaa caccaaaaac ggauccauau cccucaugug ccuggcccuc     2040

ggaggcgucc uaaucuuccu guccacagcg gucuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5177
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 1 opt

<400>  5177
gggagaaagc uuaccaugac cuccguuggc aucgugggcc uccuccuaac aaccgcaaug       60

gccgccgaag uaacccgacg uggaagugca uacuacaugu accuagaccg caacgacgca      120

ggagaagcaa ucucauuccc aaccacccua ggcaugaaca aaugcuacau ccaaaucaug      180

gaccuaggcc acaugugcga cgcaaccaug ucauacgaau gccccaugcu agacgaaggu      240

guugaaccag acgacguaga cugcuggugc aacacaacau ccaccugggu agucuacggc      300

acaugccacc acaaaaaggg agaagcacgg cgcucccgcc gagccgucac ccugcccagu      360

cacuccaccc gcaaacucca aacccgaucc caaaccuggc uagaaagccg cgaauacaca      420

aaacaccuaa uaagaguaga aaacuggaug uucagaaacc ccggauucgc ccucgccgca      480

gcagccauag cauggcuccu cggcucaucc accucacaaa aaguuauaua ccucgucaug      540

auccuacuaa uagccccagc cuauagcauc agaugcaucg gcgucuccaa ccgagacuuc      600

gucgaaggaa ugucaggagg aaccugggug gacgucgugc ucgaacacgg gggcugcgug      660

accguaaugg cacaagacaa accaacggua gacaucgagc uagucacaac aacugugucc      720

aacauggccg aaguacgaag cuauugcuac gaagcaucca ucucggacau ggccagcgac      780

ucucgaugcc ccacccaagg cgaagcauac cuggacaaac aauccgacac ccaauacgua      840

ugcaaacgua cucuaguaga ccgcggcugg gggaacgguu gcggacuguu cggaaaaggg      900

ucacugguaa ccugcgcaaa guucgcgugc ucuaagaaaa ugaccggaaa auccauccaa      960

ccagaaaacc uagaauaccg caucaugcua uccguccacg gaucccagca caguggaaug     1020

auugucaacg acaccggaca ugaaacagac gaaaaccgcg ccaaaguaga aauaacacca     1080

aacucacccc gcgccgaagc aacacucggc ggauucggcu cacucggacu cgacugcgaa     1140

ccccgcaccg gccucgacuu cucagaccug uacuaccuca caaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccacucccuu ggcacgcggg cgccgacaca     1260

gguaccccac acuggaacaa caaagaagcc cuagucgagu ucaaagacgc acacgccaaa     1320

agacagacgg uagucgugcu cggaagccag gaaggagccg uacacaccgc ccucgccggg     1380

gcacuggaag cagaaaugga cggcgcuaaa ggccgccucu cauccggcca ccuaaaaugc     1440

cgguuaaaaa uggacaaacu gagacucaaa ggaguaagcu acuccuuaug caccgccgcc     1500

uucaccuuca ccaaaauacc ggccgaaacc cuccacggua ccguaacagu agaaguccaa     1560

uacgccggaa cagacggccc cugcaaaguc ccugcccaga uggcugucga caugcaaacc     1620

cucacccccg ucggaagacu aaucacugcc aaccccguca uaaccgaauc caccgaaaac     1680

agcaaaauga uguuggaacu cgauccccca uuuggcgacu cauacaucgu gauaggcgua     1740

ggcgagaaaa agauaacaca ccacuggcac cguuccggaa guaccaucgg caaagcauuc     1800

gaagcgaccg uccgaggcgc aaaacgaaug gccguacucg gggacacggc cugggacuuc     1860

gggagcgugg gcggcgcacu aaacucccuc ggcaaaggaa uccaccaaau cuucggcgcc     1920

gccuucaagu cccucuucgg aggcaugucg ugguucuccc agaucuuaau cggaacucua     1980

cucauguggc uaggguugaa caccaaaaac ggaucaauau cccuaaugug cuuagcccuu     2040

ggaggaguac uaaucuuccu aagcaccgca gugucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5178
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 1 opt

<400>  5178
gggagaaagc uuaccaugac caguguaggc auagugggac uacuacucac uaccgccaug       60

gcagccgaag uaacaaggcg aggauccgcg uacuacaugu acuuagaccg caacgacgca      120

ggagaagcaa ucuccuuucc caccacacuc ggaaugaaca aaugcuacau ccaaauaaug      180

gaccuaggcc acaugugcga cgccacaaug uccuacgaau gcccgaugcu cgacgaagga      240

gucgagccag augacguaga cugcuggugc aacaugacca gcaccugggu cgucuacggc      300

accugccacc acaaaaaagg agaggcgcga agauccagac gggccgucac acuaccaagu      360

cacuccacgc guaaacucca gaccagaagc caaaccuggc ucgaaucgcg agaauacaca      420

aaacaccuaa uccgcgucga gaacuggaua uuccguaacc ccggauucgc ccuagcagcc      480

gccgcaaucg ccuggcuacu aggcagcuca accagccaaa aaguuaucua ccuugucaug      540

auacuccuca uugcgccagc auacucgauc agauguauug gugucaguaa ccgcgacuuc      600

guagaaggca uguccggcgg aacauggguc gacgucguuc ucgaacacgg gggaugcguc      660

accgucaugg cacaagacaa accaaccguc gacauagaac ucguaacuac aaccgucucc      720

aacauggccg aaguccgcag cuacugcuac gaagccagca ucucagacau ggcgagcgau      780

ucccgcugcc ccacgcaagg cgaagccuac cuagacaaac aaagcgacac ccaauacgua      840

uguaaacgga cccucgucga cagaggaugg ggcaacggcu gcggccuuuu cggcaaaggc      900

agccuaguaa ccugcgccaa auucgccugc agcaaaaaaa ugaccggaaa aagcauucaa      960

ccugaaaacc uagaguaccg caucaugcuc uccguccacg gaucgcaaca cuccggcaug     1020

auagucaaug acaccggaca cgaaaccgac gaaaaccgug cgaaagugga aaucaccccu     1080

aauagcccca gggccgaagc cacacucggc ggauucggca gccuagggcu agacugcgaa     1140

cccagaacag gccuagacuu cagcgaccua uacuaccuca caaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccacuaccau ggcacgcugg agccgacaca     1260

gggacucccc acuggaacaa caaagaagcg cucguagagu ucaaagaugc acacgcaaaa     1320

cggcaaacag ucguaguccu aggcucacag gaaggcgcgg uccauacagc ccucgcagga     1380

gcacucgaag cagaaaugga cggagccaaa ggccgacuaa gcagcgggca ccucaaaugc     1440

agacucaaaa uggacaaacu cagacugaaa ggcguaagcu acagccuuug cacagcagcc     1500

uucaccuuua ccaaaauacc cgccgaaacc cuacacggca ccguaacggu agaaguucaa     1560

uacgcaggca ccgacggacc augcaagguc cccgcacaaa uggccgucga uaugcagacc     1620

cuaacccccg ucggacgccu caucaccgcc aacccaguca uaaccgaaag cacagaaaac     1680

ucgaaaauga ugcucgaacu agacccaccc uucggagacu cauacaucgu aaucggaguc     1740

ggagaaaaaa aaauaacaca ucacuggcac cgauccggcu caacaauagg caaagccuuc     1800

gaagcaacag uacgaggagc gaaacggaug gccguacucg gagacacagc cugggacuuc     1860

ggcagcgucg gaggagcccu caacagcuua ggaaaaggca uccaccaaau auucggagcc     1920

gcauucaagu cccuguucgg cggcaugucc ugguucagcc aaauacuaau aggcacgcua     1980

cugauguggc ucggacucaa caccaaaaac ggcagcauca gccucaugug ccucgcgcuc     2040

ggaggcgucc ucauauuccu uucaaccgca gucucagcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5179
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 1 opt

<400>  5179
gggagaaagc uuaccaugac aagcgucggg aucgugggcc uccuacuaac gaccgcaaug       60

gcagcagagg ucacccgcag aggaagcgca uacuacaugu accuagaccg aaacgacgcc      120

ggagaagcaa ucagcuuccc cacaacccuc ggcaugaaca aaugcuacau acagaucaug      180

gaccuaggac acaugugcga cgccaccaug agcuacgaau gcccgaugcu agacgaaggc      240

gucgaaccag acgacgucga cugcuggugc aacacaacaa gcacaugggu agucuacgga      300

accugccacc auaagaaagg cgaagcgcgc agaagccggc gagcaguaac ccuaccaagc      360

cacuccacaa gaaaacuaca aaccagauca cagaccuggc ucgaaagccg ggaauacacc      420

aaacaccuca uccgcgucga aaacuggauc uucagaaauc ccggcuucgc ccuagcggca      480

gccgccaucg ccuggcuauu aggcagcagu accucccaga aaguaauaua cuuagucaug      540

auacuacuaa uagcgccagc guacucgaua cgaugcauag gagucagcaa ccgagacuuc      600

gucgaaggca ugagcggagg gacgugggug gacguaguac ucgaacacgg aggcugcguc      660

acggcgaugg cucaagacaa acccacaguc gacaucgagc uagucaccac aaccguaagc      720

aacauggccg agguaagauc auacugcuac gaggcaucca uaagcgacau ggcaagugac      780

ucaagaugcc ccacacaagg agaagcauac cucgacaaac aguccgacac ccaauacguc      840

uguaaaagaa cccuaguaga cagaggcugg ggcaacgggu gcggacuauu cggcaaaggc      900

agccucguga caugcgcgaa auucgcuugc uccaagaaaa ugacaggcaa auccauccaa      960

cccgaaaacc ucgaauaccg cauaaugcua agcguacacg ggucccaaca cuccggcaug     1020

cuggugaacg auaccggaca cgagaccgac gaaaacagag cgaaaguaga aauaacccca     1080

aauagcccgc gagcagaagc aacucuaggc ggauucggca gccuuggacu agacugcgaa     1140

ccaagaacag gccucgacuu cucagaccua uacuaccuca ccaugaacaa caaacacugg     1200

cucgcgcaca aagaaugguu ccacgauauc ccccuccccu ggcacgcagg cgcagcaacg     1260

ggaacaccac acuggaacaa caaagaagcc cucgucgagu ucaaagacgc gcacgccaaa     1320

aggcaaaccg ucguaguacu aggaucccaa gaaggagccg uccacaccgc acucgccgga     1380

gcacuagaag ccgagaugga cggcgcaaaa ggacgacucu ccagcggcca ccucaaaugc     1440

cgacucaaaa uggacaaacu gcgccuuaaa ggcgucucgu acuccuuaug cacggccgcc     1500

uucaccuuua caaaaauacc cgcagagacc guagacggca ccgucaccgu agaagggcag     1560

uacgggggaa ccgauggacc uugcaaagua ccagcccaaa uggcaguaga caugcagacc     1620

cuuacacccg uaggacgacu aauaacagcc aaccccguca uaaccgaauc caccgaaaac     1680

agcaaaauga ugcuagaacu agauccccca uucggcgacu cauacaucgu gaucgggguc     1740

ggagaaaaga aaauaaccca ccacuggcac aggagcggga gcacaaucgg caaagccuuc     1800

gaagccaccg uacggggcgc caaacgaaug gccguucucg gcgacacagc augggacuuc     1860

ggcucggucg gcggagcacu aaacagccuc ggaaaaggaa uacaccaaau aauaggcgcg     1920

gccuucaaau cacuauucgg gggcaugagc ugguucagcc aaauacugau agggacgcug     1980

cugauguggc ucggacugaa uacgaaaaac ggcuccauca gucuaaugug ccuagcccua     2040

ggaggcguac ugauauuccu cagcacagca guauccggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  5180
<211>  2275
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 1 opt

<400>  5180
gggagaaagc uuaccauggg cgccgacgcc agcaucggca ucgucucccu acuccuaaca       60

accguaaugg cagcagagau aacgcgacga ggauccgcgu acuacaugua ccuagacaga      120

agcgacgccg gcaaggccau cucauucguc acaacgcuag gcgucaacaa augccacguu      180

caaauaaugg aucucggcca caugugugac gcuaccauga gcuacgaaug cccaaugcuc      240

gacgagggcg ucgaacccga cgacguggac ugcuggugca acaccaccuc aacauggguc      300

guauauggca cgugccacca caagaaaggc gaagcccgcc gaucaaggag agccgucacc      360

cucccaagcc acucaaccag aaaacuccaa accagaagcc agaccuggcu cgagagccga      420

gaauacacaa aacaccugau aaaaguagaa aacuggaucu uccgaaaccc aggcuucgcg      480

cuagucgcag uagccauagc cuggcuccuc ggcagcucca caucacagaa gguuauauac      540

cuagucauga uacuccucau cgccccagca uacagcaucc gcugcauagg cguaagcaac      600

cgcgacuucg ucgaggggau guccggagga acgugggucg acguagugcu agaacacggu      660

ggcugcguaa ccgucauggc gcaagacaaa ccgaccgucg acaucgagcu agucacaacg      720

acaguaucaa acauggccga aguacgaagc uacugcuacg aagccagcau cagcgacaug      780

gccucagaca gcagaugucc aacccaagga gaagcauacc ucgacaaaca aagcgacacg      840

caauacgucu gcaaacgcac ccuaguagac cguggauggg gcaacggcug cggccuguuu      900

ggaaaaggcu cacuggucac augugccaaa uuuacaugca gcaagaaaau gacgggcaaa      960

agcauacaac cagaaaaccu agaauaccgc aucaugcuaa gcguccacgg aagccaacac     1020

uccggaauga uagucaacga cgaaaaccga gcaaaagucg aaguaacacc caacucacca     1080

agagcagaag caacacucgg cggcuucgga agccugggcc uagacugcga acccagaacc     1140

ggacuagacu ucagcgaccu guacuaccuc accaugaaca acaaacacug gcucguccac     1200

aaggaauggu uccacgacau accacuacca uggcacucag gagccgacac cgaaacccca     1260

cacuggaaca acaaagaagc acugguggaa uuuaaagacg cacacgccaa aagacagacc     1320

gucguagucc ucgggagcca agaaggcgcc guccauacag cacuggccgg agcccuagag     1380

gccgaaaugg acggagcaaa aggacgccua ucauccggcc accucaaaug ccgccucaaa     1440

auggacaaac uccgacuaaa aggcguaucc uacucccugu gcaccgccgc auucacauuc     1500

acaaaaguac cagccgaaac acugcacggc acggugaccg uagaagucca auacgcagga     1560

cgcgacggcc caugcaaggu ccccgcacaa auggcaguag acaugcagac auuaacgcca     1620

gucggaagac ucauaaccgc caaccccguc auaacugaaa gcaccgaaaa cucaaaaaug     1680

augcuugaac uagacccacc cuucggagac uccuauaucg ucauaggagu aggcgacaag     1740

aaaauaacac accacuggca cagaucaggc agcauaaucg ggaaagccuu cgaggccaca     1800

guccgcggcg ccaaacgcau ggcaguccua ggggacacag caugggacuu cggcuccgug     1860

ggcggaguuu ucaacucccu aggaaaggga auccaccaaa ucuucggcgc cgccuucaaa     1920

ucacucuucg gaggaauguc cugguucucu caaauccuaa ucggaacccu acucgucugg     1980

cucggccuaa acaccaagaa cggcagcaua agccuaaccu gccucgcgcu cggaggcgua     2040

augauauucc ucucaacugc ggucagcgca ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2220

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2275


<210>  5181
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 1 opt

<400>  5181
gggagaaagc uuaccauggg agcagacacc agcaucggaa ucauaggacu acuccuaaca       60

acagcuaugg cggccgaaau cacgagaaga ggaagcgcau acuauaugua ccucgaccgu      120

uccgacgcag gcaaagcgau aucauucgca accacaaugg gcgugaacaa augccacguc      180

caaaucaugg accuagguca caugugugac gccaccaugu cuuacgaaug ccccaugcuc      240

gacgaaggag ucgaacccga cgacguagac ugcuggugca acaccaccuc cacauggguu      300

guguacggaa caugccauca caagaaagga gaagcccggc cgucccgccg cgcaguaacg      360

cucccgagcc acuccaccag aaaacuacaa acccgcucac aaacauggcu cgaaagucgc      420

gaguacacaa aacaucucau aaaagucgaa aacuggauac uacgcaaccc aggcuucaca      480

guggccgcgc ucgccauggc cuggcuacug ggaaacagua cgagccagcg cguaaucuac      540

cucguaauga uucuccugau cgcacccgca uacuccauac gcugcaucgg cguuagcaac      600

cgcgacuucg uagaaggaau guccggagga accuggguag acgugguccu agaacacgga      660

ggaugcguca ccgucauggc ccaagacaaa ccaacagucg acauagagcu aguaacaacc      720

acggucucca acauggccga agucagaagc uacugcuacg aagcauccau cagcgacaug      780

gcaagcgacu cccgcugucc cacacaaggg gaagccuacc ucgacaaaca guccgacaca      840

caauacgucu gcaaacgaac ccucguggau cgcggauggg gcaacggaug cggacucuuc      900

ggaaaaggaa gccuagugac cugugcaaag uucacgugcu ccaagaaaau gacaggaaaa      960

uccauacaac ccgaaaaccu cgaauaccgg aucaugcuca gcguccacgg aucccagcac     1020

agcggaauga uagugaacga cauaggccac gaaaccgacg aaaaccgagc aaaaguggaa     1080

gucaccccua auucaccccg cgcagaagca acacugggcg gcuucggcag ccucggccua     1140

gacugcgaac cgcgcaccgg acuagacuuu ucagaccugu acuaucuaac aaugaacaac     1200

aaacacuggc uuguccauaa agaaugguuc cacgacaucc cccucccaug gcaugcaggc     1260

gcagacacag gcacacccca cuggaacaac aaagaggcac ucguggaguu caaagacgca     1320

cacgccaaaa gacaaaccgu cguaguacuc ggcucccaag aaggagcagu ccacaccgcu     1380

cuggcaggag cauuggaggc agaaauggac ggcgcgaagg gccgacucuu cucaggacac     1440

cucaaaugcc ggauaaaaau ggacaaacua aggcuaaaag gcgucagcua cucacucugc     1500

accgccgcau ucacauucag caaagucccg gcagaaacgc uccacggaac agucacggug     1560

gaggugcaau acgccggccg agacggaccc ugcaaagucc ccgcccaaau ggcaguagac     1620

augcaaacgc ucacccccgu aggaagacuu aucacggcca acccaauaau aacagaaucc     1680

accgaaaaca gcagaaugau gcucgagcuc gacccucccu ucggcgacuc auacauaguu     1740

aucggaguag gcgacaagaa aaucacgcac cacuggcaca ggucaggcag cacaaucgga     1800

aaagccuucg aagccaccgu ccgcggcgca aaaagaaugg ccguacucgg cgacacagcc     1860

ugggacuuug gaagcgucgg cggagucuuc aacucacuag ggaaaggagu ucaccaagua     1920

uucggagccg ccuucaaauc gcuauucggc gggauguccu gguucucgca aauccucauc     1980

ggaacccuuc uagucuggcu cggacugaac accaaaaacg gaagcaucuc ccuagcaugc     2040

cuagcccucg gcggugcaau gauauuccua agcacggccg uaagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  5182
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 1 opt

<400>  5182
gggagaaagc uuaccauguc gcaaaagguc auauaccugg ucaugaucuu acucauagcc       60

ccggcauaca gcauaagaug uauaggaguc ucgaacagag acuucgucga aggaaugagc      120

ggaggcacgu ggguggacau aguccuggaa cacggaggau gcgugaccgu gauggcgcaa      180

gacaaaccga ccguggacau agaacuggua acaaccacgg ucucgaacau ggccgagguc      240

aggagcuacu gcuacgaagc aagcauaagc gacauggcaa gcgauaguag augcccgaca      300

caaggcgagg cauaucuaga caaacaaagc gauacccaau acguauguaa gcgcacauua      360

guggaccgag gauggggaaa cggcugcgga cuguucggga aaggaagccu agucacgugu      420

gcgaaguucg cgugcagcaa gaaaaugacc ggcaaaucga uacaacccga gaaccuagaa      480

uacagaauaa ugcuaagcgu acacgggagc cagcacucgg gcaugaucgu aaacgacacc      540

ggacacgaga ccgacgaaaa ccgagcgaaa guggaaauaa cacccaacuc gccccgagca      600

gaggccaccc uaggaggauu cggcucgcuc gggcuagacu gcgagcccag gacuggucua      660

gacuucucag acuuguacua ccucacgaug aacaacaagc acuggcuagu acacaaggaa      720

ugguuccacg acauaccacu gcccuggcac gcgggagccg acacagguac accccacugg      780

aauaacaagg aggcccucgu ggaguucaaa gacgcccacg caaaacgcca aaccguggua      840

guacucggca gccaggaagg ggccguccac acggcccugg caggggcacu ggaagcggaa      900

auggacgggg caaagggcag acuaucgagc ggacaccuaa aaugucgccu aaaaauggac      960

aagcuaaggc ucaagggcgu aaguuacagc cuaugcacgg ccgcauucac guucacgaaa     1020

auacccgcag aaacgcuaca cggcacgguc acggucgagg uccaauacgc gggcacagac     1080

ggacccugca aagugcccgc gcaaauggcc guggacaugc aaacgcucac gcccgucgga     1140

cggcucauaa ccgcaaaccc gguaauaacc gaaaguacgg aaaacagcaa aaugaugcuc     1200

gaguuagacc caccauucgg ggacucauac auaguaauag gcgugggcga gaaaaagaua     1260

acgcaccacu ggcaccgcuc gggaucuacc aucggcaagg cuuuugaagc aacaguacgc     1320

ggggcaaaac gcauggccgu gcucggggac acggccuggg acuuuggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5183
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 1 opt

<400>  5183
gggagaaagc uuaccauguc gcaaaaaguc aucuaccuag ucaugauccu acucauagcc       60

cccgccuaca gcauccgcug caucggaguc ucaaaccgcg acuucguuga aggcauguca      120

ggaggcacau gggucgacgu gguacuagaa cacggcgggu gcgucacagu cauggcccaa      180

gacaaaccca caguggacau cgaacugguc accaccacag uaucaaacau ggcagaaguu      240

agaagcuacu gcuacgaggc aagcauaucc gacauggcca gcgacucaag augcccaaca      300

caaggcgaag ccuaccugga caaacaaagc gacacucaau acguaugcaa acgaacgcua      360

guggacagag gauggggaaa cggauguggc cucuucggca aaggcagccu cgucacaugc      420

gcaaaauucg ccugcucaaa gaaaaugacc ggaaaaucca uucaacccga gaaccuagaa      480

uacagaauca ugcuauccgu gcacggcagc caacacuccg gaaugauagu caacgacacc      540

ggccacgaaa ccgacgaaaa ccgcgcaaaa guggaaauaa ccccaaacuc acccagagcc      600

gaagcaacac uaggcggcuu cggcucccuc ggacuagacu gcgaacccag aaccggccuc      660

gacuucucag accuauacua ucuaaccaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauaccccu acccuggcac gcaggggccg auacaggcac accccacugg      780

aauaacaaag aagcccucgu agaguucaaa gaugcacaug caaaacgaca gaccguaguc      840

gugcucggca gucaggaagg cgccguccac acggcccugg ccggagcccu cgaagcagaa      900

auggacggcg caaaaggccg acucuccagc ggacaccuaa aaugccgccu aaaaauggac      960

aaacuacgcc ucaaaggcgu cagcuacagc cucugcacag cagcauucac guucacaaaa     1020

auaccagcag aaacacucca cggcacagua acagucgaag uccaguacgc cggcacagac     1080

ggacccugca aaguaccagc acaaauggca guagacaugc aaacgcucac gcccguagga     1140

cgccuaauca ccgcaaaucc agucaucacc gaaagcaccg aaaacagcaa aaugaugcuc     1200

gaacucgauc ccccauucgg cgacucguac auagucaucg gcgugggaga aaagaaaauc     1260

acacaccacu ggcaccgcuc cggaagcacc auaggcaaag ccuucgaagc caccguacgc     1320

ggagccaaac gcauggcagu acucggagac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5184
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 1 opt

<400>  5184
gggagaaagc uuaccauguc gcaaaaagua auauaccuag ucaugauccu acucaucgcc       60

ccagcuuaca gcauacgcug caucgggguc ucaaacaggg acuucgucga aggcaugagc      120

ggaggaacau ggguggacgu gguacuagaa cacggcggau gcguaaccgu aauggcgcaa      180

gacaagccua ccguagacau cgaacuagua accaccacag ucuccaacau ggccgaaguc      240

aggaguuacu gcuacgaagc aagcauaagc gacauggcga gcgacagccg gugcccgaca      300

caaggugaag cauaccuaga caaacaaagu gacacccaau acgucugcaa aaggacccua      360

guagacaggg gcuggggcaa cggcugcggu cuauucggaa aaggcagccu agucacaugc      420

gccaaauuca ccugcagcaa aaaaaugacc ggcaaaucaa uccaacccga aaaccucgaa      480

uaccgcauaa ugcugagcgu gcacggguca caacacucgg gaaugaucgg auacgaaaca      540

gacgaagacc gcgcaaaagu agaaguaaca ccgaacucgc caagagcaga agccacccuc      600

ggaggauucg gcucucucgg acucgacugu gaaccaagaa ccgggcuaga cuucucagac      660

cucuacuacc uaacgaugaa caacaaacac uggcucgugc acaaagaaug guuccacgac      720

auaccccugc ccuggcacgc aggcgcagac acgggcaccc cacacuggaa caacaaggaa      780

gcacuaguag aauucaaaga cgcccacgcc aagcgucaaa caguagucgu gcucgggagc      840

caagaagggg ccguacacac ggcccuagca ggggcccuag aagcagaaau ggacggggca      900

aaaggaagac uauucuccgg acaccugaaa ugccgccuaa aaauggacaa acucagacua      960

aagggcguaa gcuacagccu augcacagcc gcauucacau ucacaaaagu ccccgcagaa     1020

acacuacacg gcaccgucac cgucgaaguc caauacgcgg gcacagacgg cccauguaaa     1080

auaccaguac aaauggcagu agacaugcaa acacucacgc cggugggcag acucauaaca     1140

gcaaacccag ucauaaccga aagcacagaa aacaguaaaa ugaugcuuga acucgaccca     1200

cccuucggug acucguacau cgucauagga guaggcgaca aaaaaaucac gcaccacugg     1260

caccgaucag ggucaaccau uggcaaagca uucgaagcaa caguacgcgg ggccaaacgc     1320

auggccgucc uaggggacac cgccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  5185
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 1 opt

<400>  5185
gggagaaagc uuaccauguc ccaaaaagua auauaccuag uaaugauccu ccucaucgca       60

ccggccuaua guauccguug cauaggaguc agcaacagag acuuuguaga aggcaugagc      120

ggcggcaccu gggucgacgu cguccuggaa cacggaggau gcgugaccgu aauggcacaa      180

gacaaaccga ccguggacau cgaacugguc acaacaacag ucucaaacau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcauaagc gacauggcga gcgacagccg augcccaacc      300

cagggagaag cauaccucga caaacagagc gacacacaau acguaugcaa acgaacgcuc      360

guagacaggg gcuggggcaa cggaugcggc cuauucggaa aagggagccu agucacgugc      420

gcgaaauucg ccugcagcaa gaaaaugaca ggaaaaucaa uccagccaga aaaccuagag      480

uaccgaauca ugcugagcgu acacgggagc caacacucag gcaugaucgu aaacgacacc      540

ggacacgaaa ccgacgaaaa ccgcgcaaaa guagaaauaa ccccaaacuc accaagggcc      600

gaagcaacgc uaggcggauu cggcucacuc ggacucgacu gcgagccaag aaccggacuc      660

gacuucagug accuguacua ccucacaaug aacaacaaac acuggcuagu acacaaagaa      720

ugguuccacg acauaccacu ccccuggcac gcgggcgcag acacgggcac accacacugg      780

aacaacaaag aagcccucgu agaguucaaa gacgcacacg ccaaacgaca gaccguggua      840

guacucggaa gccaggaagg ggccgugcac accgcccugg ccggcgcccu ggaagccgaa      900

auggacggag caaaaggccg ccucucaagc gggcacuuaa aaugucgccu aaaaauggac      960

aaacucaggu uaaaaggcgu aagcuacagu cuaugcacgg ccgcauucac auucaccaaa     1020

auaccagcgg aaacgcuaca cggcacaguc acggucgaag uccaauacgc gggcacagac     1080

gggcccugua aaguaccagc acaaauggcc guagacaugc aaacacuaac accggucggc     1140

cgacucauaa ccgcgaaccc agucauaacc gaaagcacag aaaacaguaa gaugaugcua     1200

gaacuagauc ccccauucgg agacagcuac auagucauag gcguagguga gaaaaagaua     1260

acacaccacu ggcacagaag cggaucaacc auaggaaagg ccuucgaagc aaccgugaga     1320

ggggcaaaaa gaauggccgu acucggcgac accgccuggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5186
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 1 opt

<400>  5186
gggagaaagc uuaccauguc gcaaaaagua aucuaccuag ucaugaucuu gcuaauagcc       60

ccggccuaca gcauacgcug caucggagua ucaaacaggg acuucgucga aggaaugagc      120

ggaggcacgu ggguagacgu gguacuagaa cacggcggau gcgucaccgu aauggcgcaa      180

gacaaaccaa ccguggacau cgaacucgua acgaccacgg uaucaaacau ggccgaagua      240

agaagcuacu gcuacgaagc aagcaucagc gacauggcaa gcgauagccg gugcccgacc      300

caaggagaag cauaccuaga caaacagagc gacacccaau acguaugcaa acgaacccua      360

guagacaggg gcuggggcaa cggcugcgga cuauucggaa aagggagccu agucacaugc      420

gccaaauuca caugcagcaa gaaaaugacc gggaaaucaa uccagcccga aaaccuagaa      480

uaccgcauaa ugcuaagcgu gcacgggagu cagcacucug gcaugaucgu gaacgacacg      540

ggauacgaaa cagacgaaaa cagagcaaaa guagaaguaa caccaaacuc cccaagagcc      600

gaagccacgc ucgggggauu cggaucgcuc ggacuagacu gcgaaccccg uaccgggcua      660

gacuucucag accuauacua ccuaacaaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acauaccacu gcccuggcac gcgggcgccg acacuggaac accacacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacaug caaagagaca gaccgugguc      840

guacugggaa gccaagaagg agcaguacac acagcacuag caggggcacu agaagcggaa      900

auggacgggg ccaaaggaaa acuauucagu ggacacuuaa agugcagacu aaaaauggac      960

aaacuaagac ucaagggcgu aagcuacagc cuaugcacag cagcguucac guucacgaaa     1020

gugccagcgg aaacgcuaca cggaaccguc acugucgaag uccaauacgc gggcacagac     1080

gggcccugca aaauccccgu ccaaauggcc guagacaugc aaacgcuaac acccguggga     1140

agacuaauaa ccgcaaaccc agucauaaca gaaagcacgg aaaauagcaa aaugaugcuc     1200

gaacuagacc caccguucgg agacagcuac auaguaauag gcguaggaga caagaaaauc     1260

acccaccacu ggcaccgcuc gggcucaacc auaggaaaag ccuucgaagc gaccguacgc     1320

ggagccaaac gaauggccgu acuaggagac acggcauggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5187
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 1 opt

<400>  5187
gggagaaagc uuaccauguc acagaaagua auauaccuag uaaugauacu ccucauagcc       60

ccggccuacu ccauacgcug caucggaguc agcaacagag acuucguaga gggaaugagc      120

ggcggaacau ggguagacgu gguccuagaa cacggcggau gcguaacagu aauggcacaa      180

gacaaaccaa ccguggacau agaauugguc acaaccacag uaucgaacau ggccgaaguc      240

agaaguuacu gcuacgaagc aagcauaagc gacauggcaa gcgacagccg cugccccacc      300

caaggagagg cauaccucga caaacaaagc gacacacaau acguaugcaa acgaacacuc      360

guagaccggg gcugggguaa cggcugcggc cuauucggga aagggagccu agucacaugc      420

gcgaaguuca caugcagcaa gaaaaugacc ggcaaaagca uccagcccga gaaccuagaa      480

uacagaauaa ugcucagcgu gcacgggagc caacacucgg gcaugaccgu aaacgacauc      540

ggcuacgaaa cagacgaaaa ccgcgccaaa guagaaguaa ccccgaacuc gccaagagcc      600

gaagccaccc uagguggauu cggaucccuc ggacucgacu gcgaaccaag aaccggacuc      660

gacuucucgg accuauacua ccucacgaug aacaacaaac acuggcuagu acacaaagaa      720

ugguuccacg acauaccccu acccuggcac gccggagcag acacaggcac gccacacugg      780

aacaacaaag aagcgcuagu agaguucaaa gacgcccacg ccaaacgaca aacaguggua      840

guguuaggaa gccaagaagg ggcaguccac acagcccugg caggagcacu cgaagcagaa      900

auggacggag ccaaaggcaa acucuuuagc ggacaccuca aaugccgccu aaaaauggac      960

aaacuccgcc uaaagggcgu aaguuacagc cugugcacgg cagcguucac cuucacaaaa     1020

gugcccgcgg aaacacucca cggcaccguc acggucgaag uccaauacgc aggaacggac     1080

ggacccugca agauacccgu acaaauggca guagauaugc agacucucac ccccgucgga     1140

agacucauaa ccgccaaccc gguaauaaca gaaagcacag aaaacagcaa aaugaugcuc     1200

gaacucgacc caccauucgg ggacucauac auagugaucg gcgugggcga caagaaaauc     1260

acccaccacu ggcaucgcuc gggcagcacc auaggcaaag ccuucgaagc aacaguccgc     1320

ggggcaaaac gaauggccgu auuaggggac accgcauggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5188
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 1 opt

<400>  5188
gggagaaagc uuaccauguc ccaaaagguc auauaccuug uaaugauccu acucauagcu       60

ccagcauaca gcauacgcug cauaggcgua ucaaacagag acuucguaga aggcaugagc      120

ggaggaacau gggucgacgu aguacucgaa cacggaggcu gcgucacagu aauggcacag      180

gacaaaccga ccguggacau agaacucguc acaaccacag ucuccaacau ggccgaagua      240

agaagcuacu gcuacgaagc aagcaucagc gacauggcgu cagacagccg augcccaaca      300

caaggcgaag ccuaccugga caaacagagu gacacccagu acgugugcaa acggacacuu      360

guagacagag gcuggggcaa cggcuguggc cuguucggaa aagggagccu agugaccugc      420

gcaaaauuca caugcagcaa gaaaaugaca ggcaaaucaa uccaaccaga aaaccucgaa      480

uacagaauaa uguuauccgu ccacggaagc caacacuccg gcaugauagu aaacgacgaa      540

aaccgcgcca aaguagaagu aacaccaaac ucacccagag ccgaagccac gcucggcggc      600

uucggaucgc uaggccuaga cugcgaaccc cgaacagggc ucgacuucag cgaccucuac      660

uaccucacga ugaacaacaa acacuggcua guacacaaag aaugguucca cgacaucccc      720

cuacccuggc acgcaggcgc agauacaggc acaccgcacu ggaacaacaa ggaagcgcuc      780

guggaguuca aagacgcaca cgccaagaga caaaccguag ucguacuagg aagucaggaa      840

ggcgccguac acaccgcccu cgccggagca cucgaagcag aaauggacgg agcaaaagga      900

agacucuuca gcggacaccu caaaugccgc cucaaaaugg acaagcuaag acucaaaggc      960

guaagcuaca gccuaugcac agccgccuuc acauucacaa aaguacccgc cgaaacacuc     1020

cacggcacag ucaccgucga aguccaauac gccggcacag acggacccug caaaguaccc     1080

gcacaaaugg ccgucgacau gcaaacgcuc acacccgucg gcaggcucau aaccgcaaac     1140

ccggucauca ccgaauccac ggaaaacagu aaaaugaugc ucgaacuaga ucccccauuc     1200

ggcgacucgu acauaguaau aggcgucggc gacaagaaaa uuacacacca cuggcaccga     1260

uccggaagca ccauaggcaa agccuucgaa gcaacagucc gcggcgccaa acgcauggcc     1320

guacuagggg acacggcaug ggacuuuggc ucgugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  5189
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 1 opt

<400>  5189
gggagaaagc uuaccauguc acaaaaaguc auauaccuag uaaugauacu gcuaauagca       60

ccagcauaua gcauacgcug caucggaguc uccaacagag acuucgucga gggcaugagc      120

gggggaacgu ggguggacgu aguacucgaa cacggcggau gcgucaccgu cauggcacaa      180

gacaaaccaa caguagacau agaacugguc acaaccaccg ucucaaauau ggccgaaguc      240

agauccuacu gcuacgaagc aagcaucucc gacauggcaa gcgacagccg augcccaacc      300

caaggcgaag ccuaccucga caaacaauca gacacgcaau acguuugcaa acgaacccuc      360

gucgaccgag gcuggggcaa cggcugcggc cucuuuggga aaggaagccu aguaacaugc      420

gcgaaauuca caugcuccaa gaaaaugacc ggcaaaucaa uccaaccaga gaaccuagaa      480

uaccgaauca ugcugagcgu acacggcagc caacacucag gaaugauagg guacgaaacc      540

gacgaaaauc gagccaaagu cgaagucacc ccaaacagcc cacgagccga agccacccuc      600

ggaggcuucg gaucacucgg acucgacugc gaaccccgga ccgggcucga cuucucagac      660

cuguacuacc ucacaaugaa caauaaacac uggcucguac auaaagaaug guuucacgac      720

auaccccucc ccuggcacgc cggcgcagac accggcacac cacacuggaa caacaaagaa      780

gcacucgugg aguucaaaga cgcccacgcc aaacgccaga ccgucgucgu acuaggcuca      840

caagaaggag caguccacac agcccuagca ggggccuuag aagcggaaau ggacggcgca      900

aaagggaaac ucuucagcgg acaccugaaa ugccgccuaa aaauggacaa acuacgacuc      960

aaaggcguaa gcuacagucu augcacggcc gcauucaccu ucaccaaagu cccagcagaa     1020

acacuccacg gcacagucac aguagagguu caauacgcag gcacagacgg ccccugcaaa     1080

auacccgucc aaauggccgu agacaugcaa acccuaaccc ccgucggcag acuaaucaca     1140

gccaacccgg ucaucacaga auccacagaa aacagcaaaa ugaugcucga acucgaccca     1200

ccauucggag acucuuacau agucauagga guaggcgaca agaaaaucac gcaccacugg     1260

caccgauccg gcuccacaau aggcaaagcc uucgaagcaa ccgugcgcgg cgcaaaaaga     1320

auggcagucc ucggagacac cgccugggac uucggcucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  5190
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 1 opt

<400>  5190
gggagaaagc uuaccauguc gcagaaaguc auauaccugg ucaugauccu gcucauagcc       60

ccagccuaca gucuacgaug caucggagua agcaacagag acuucgucga aggaaugagu      120

ggaggcacau ggguggacgu cguccuggaa cacggcgggu gcgucacagu aauggcacaa      180

gacaaaccca ccguagacau cgagcuaguc acaaccacag ucuccaacau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcauauca gacauggcaa gcgacagcag augcccgacc      300

cagggggagg ccuaccucga caaacaaagc gacacccaau acgucugcaa acgcacccua      360

guggacaggg gcuggggcaa uggcugcggc cuguucggaa aaggcagccu ggucacaugc      420

gcaaaauucg ccugcucaaa gaaaaugaca ggaaaaagca uccaacccga aaaccuggaa      480

uaucgcauaa ugcuaucagu ccacggcagc cagcacucag gcaugauagu gaacgacacc      540

ggacacgaaa cagacgaaaa ccgcgcaaaa guagaaauaa ccccaaacuc accccgagcc      600

gaagcaacgc uuggcggcuu cggaucacuu ggacucgacu gcgaaccaag aaccggacua      660

gacuucagug accucuacua ccucaccaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauaccauu acccuggcac gcgggcgcag acacuggaac accccacugg      780

aacaacaaag aagcgcucgu ggaguucaaa gaugcccacg ccaaacgcca gaccgugguc      840

guacucggga gccaggaagg agcaguacac acggcccuag caggggcccu agaagcagaa      900

auggacggag caaaaggcag acucucaagc ggacacuuaa aaugccgccu gaaaauggac      960

aaacuacggc uaaaaggagu aaguuacagc cucugcacag ccgcauucac guucacgaag     1020

auaccggccg aaacacucca cggaacaguc acgguagaag uccaauacgc gggcacggac     1080

gggccaugca aaguacccgc gcaaauggca guagacaugc aaacgcuaac acccgugggc     1140

cgacucauaa cagcaaaccc cgucauaacc gaaagcaccg aaaacagcaa aaugaugcuc     1200

gaacucgauc ccccguucgg agacucauac auaguaauag gcguaggaga aaagaaaauc     1260

acgcaccacu ggcaccgcuc aggaucaacc auaggcaaag ccuucgaagc cacaguaaga     1320

ggggccaaac gcauggcagu gcuaggcgac acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5191
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 1 opt

<400>  5191
gggagaaagc uuaccaugag caaaaaaguc auauaccucg ucaugauccu ccuuauagcc       60

cccgccuacu caauccgcug caucggugua agcaaccgcg acuucguuga aggaaugucc      120

ggcggaaccu gggucgacgu cguccucgaa cacggcggcu gcgugacugu cauggcacaa      180

gacaaaccca ccguagacau cgaacucguc acaaccaccg ucucgaacau ggccgaggua      240

cggagcuacu gcuacgaagc auccauauca gacauggccu ccgacuccag augcccaacu      300

caaggagaag ccuaccucga caaacaaucc gacacccaau acguaugcaa acgaacccua      360

guggaccgag gauggggcaa cggcugcgga cucuucggca aaggcucccu cgugacaugc      420

gccaaauucg ccugcucaaa aaaaaugacc ggaaaaucca uccaacccga aaaccucgaa      480

uacaggauca ugcuaagcgu ccacggauca caacacuccg gaaugaucgu caacgacaca      540

ggacacgaaa cagacgaaaa ccgagcaaaa guagaaauaa caccaaacuc ccccagagcc      600

gaagccaccc ucggcggcuu cggcucccua ggccuagacu gcgaaccaag aacgggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg auaucccccu ccccuggcac gccggcgccg acaccgggac cccccacugg      780

aacaacaaag aagccuuagu cgaauucaaa gacgcacacg caaaaagaca aaccgucguc      840

guccucggaa gccaagaagg cgcaguccac acagcccucg cgggagcccu agaagcagaa      900

auggacggag cgaaaggccg ccucuccagc ggccaccuca aaugccgccu caaaauggac      960

aaacuaagac ugaaaggcgu cuccuacucc cucuguaccg cagcauucac cuucacaaaa     1020

auccccgcag aaacccucca cggcaccguc accguagaag uccaauacgc aggcacggac     1080

ggccccugca aaguaccagc ccaaauggcc guagacaugc aaacccuaac ccccgucggc     1140

cgacucauaa ccgcaaaucc agucaucaca gaaucgaccg aaaacagcaa aaugaugcuc     1200

gaacuugacc ccccauucgg ggacuccuac auaguaaucg gcgucggcga aaaaaagaua     1260

acccaccacu ggcaccgcuc uggaucuacc aucggcaaag ccuucgaagc caccguacgc     1320

ggagcaaaac gcauggccgu ccucggcgac accgcauggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5192
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 1 opt

<400>  5192
gggagaaagc uuaccauguc acaaaaagua aucuaccugg ucaugauccu acucauagcc       60

cccgcauaca gcauacgcug cauaggaguc ucaaaccgcg acuucgucga aggaaugagc      120

gggggaacgu ggguagacgu aguccuagaa cacggcggau gcguaaccgu aauggcgcaa      180

gacaaaccaa ccguagacau agagcuaguc acaacaacag ucucgaacau ggcagaaguc      240

aggagcuacu gcuacgaagc aaguauaagc gacauggcca gcgauucccg augcccgaca      300

caaggagaag cauaccuaga caaacaaagc gacacacaau acguaugcaa acgcacccua      360

guagaccgug gcuggggcaa cgguugcgga cuauucggga aaggaagucu ggucacaugc      420

gcaaaauucg caugcagcaa gaaaaugacc ggaaaaucga uacagcccga aaaccuagaa      480

uaccgaauaa ugcuaagcgu acacgggagc caacacucgg gaaugauagu aaacgacacc      540

ggacacgaga ccgacgaaaa cagagcaaaa guagaaauaa caccaaacuc gccucgagcc      600

gaagcaacac ucggaggcuu cggaucacuc ggacucgacu gcgagcccag aaccggccuc      660

gacuucucgg accuauacua ccucacaaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acauaccccu gcccuggcac gcaggcgccg acacgggaac gccccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacacg ccaaacgcca aaccgugguc      840

gugcucggga gccaagaagg agcagugcac acagcacugg ccggagcccu ggaagccgaa      900

auggacggag caaaaggaag gcucucaagc ggacaccuaa aaugccgccu gaaaauggac      960

aaguuaaggc uaaaaggagu aucauacagc cucugcacgg ccgcguucac cuucacaaaa     1020

auacccgcag aaacgcuaca cggcaccguc acagucgaag uacaguacgc gggaaccgac     1080

ggcccaugca aaguacccgc acaaauggcc guggacaugc aaacacuaac gccagugggc     1140

aggcuaauaa ccgcaaaccc agucauaacg gaaagcacgg aaaauagcaa aaugaugcuc     1200

gaacucgacc caccguucgg agacucguac auaguaauag gcguaggcga aaagaaaaua     1260

acacaccacu gguaccgcuc gggcucaacc auaggcaaag cauucgaagc aaccgugcga     1320

ggcgccaaac gcauggcugu acucggagac acagcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5193
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 1 opt

<400>  5193
gggagaaagc uuaccaugag ccaaaaaguc auauaccuag ucaugauccu acuaaucgcc       60

ccagcauacu caauacgcug caucggcguc agcaaucgag acuucgucga aggcaugagc      120

ggcggcacau gggucgacgu aguccucgaa cacggcggcu gcgucaccgu cauggcccaa      180

gacaaaccaa ccguagacau agaacucguc acaacaacag ucucaaacau ggccgaaguc      240

agaagcuacu gcuacgaagc cuccauaagc gacauggcau ccgacucacg cugcccaaca      300

caaggcgaag cauaccucga caaacagucc gacacgcaau acguaugcaa acgcacccua      360

guagacaggg gcugggggaa cggaugcggc uuauucggca aaggcagccu cgucacaugc      420

gcgaaauucg ccugcuccaa gaaaaugacc ggcaaaucca uacagccaga gaaccuagaa      480

uaccgaauaa ugcucuccgu ccacggauca caacacagcg gaaugaucgu aaacgacacc      540

ggccacgaga ccgacgaaaa ccgcgccaaa gucgaaauca cccccaacag cccacgagcc      600

gaggccacac ucggaggcuu cggcucgcua ggacucgacu gugaaccuag aaccggccuc      660

gacuucucag accucuacua ccuaacgaug aacaacaaac acuggcuagu gcacaaagaa      720

ugguuccacg acauaccacu gcccuggcac gcaggcgcag acaccggcac gccacacugg      780

aacaacaagg aagcccuagu agaguucaaa gacgcccacg ccaagcgaca aaccgucgua      840

gugcucggcu cccaagaagg agccguccac acagcucuag ccggagcacu agaagcagaa      900

auggacggag ccaaaggaaa acucucaagc ggccaccuaa aaugccgacu caaaauggac      960

aaauuacgac uaaaaggcgu auccuacucc cuaugcacag ccgcauucac auucacaaaa     1020

auucccgccg aaacgcuaca cggcacaguc acaguggaag uacaauacgc cggcacagac     1080

ggaccaugca aaguaccagc ccaaauggcc gucgacaugc aaacgcugac acccgucggc     1140

agacucauaa cagccaaccc cgucauaacc gaaaguaccg aaaacucaaa aaugaugcuc     1200

gaacucgacc caccauucgg agacagcuac auagucaucg gcgucggaga aaagaaaaua     1260

acccaccacu ggcaccgaag cggcuccaca aucggaaaag ccuucgaagc cacaguacgg     1320

ggagccaaac guauggccgu acucggcgau accgcauggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5194
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 1 opt

<400>  5194
gggagaaagc uuaccauguc acaaaaaguc auauaucuag ucaugauccu gcuaauagcc       60

ccagccuaca gcauccgcug caucggagua ucgaaccgcg acuucgucga aggcaugagc      120

ggaggcaccu ggguagacgu aguccuagaa cacggcggau gcguuaccgu aauggcacaa      180

gacaagccaa ccguagacau cgagcuaguc accaccaccg uuuccaacau ggcagaagua      240

cgaagcuacu gcuacgaagc aaguauaagc gacauggcgu ccgacagccg gugccccaca      300

caaggcgaag ccuaccucga caaacaaagc gacacacagu acguuugcaa gcggacgcug      360

guggaccgcg gcuggggcaa cggaugcgga uuguucggaa aaggaagccu agucacaugc      420

gcgaaauucg caugcagcaa gaaaaugaca ggcaaaucaa uacagccaga aaaccuagaa      480

uacagaauaa ugcuaagcgu acacggaagu caacacagcg gcaugaucgu caacgacaca      540

ggccacgaga ccgacgaaaa cagagcaaaa guagaaauaa ccccaaacuc gccaagagcc      600

gaagccacac ucggaggauu cggcucgcuc ggacucgacu gcgagcccag aaccggacuc      660

gacuucuccg accuauacua ccucacaaug aacaauaaac acugguuagu acacaaagaa      720

ugguuccacg acaucccacu gcccuggcac gcaggagccg acacaggcac accccacugg      780

aacaacaagg aagcucuagu agaguucaaa gacgcccacg cgaaacguca aaccguagua      840

guacuaggca gccaagaagg cgcagugcac acagcccucg ccggcgcacu agaagcggaa      900

auggacggcg caaaaggcag auuaagcagc ggccaccuaa aaugccgccu aaagauggac      960

aaacuaagac ucaaaggagu aagcuacagc cuaugcacag cagcauucac cuucaccaaa     1020

auacccgccg aaacacuaca cgggacagua acagucgaag uacaguaugc aggcacagac     1080

ggccccugca aaguacccgc acaaauggcc guggacaccc aaacacuaac ccccguaggc     1140

aggcuaauaa ccgccaaccc aguaaucacc gaaagcacag aaaacagcaa aaugaugcuc     1200

gaacuagauc ccccauucgg agacuccuac aucgucauag gagucggcga aaagaaaauc     1260

acucaucacu ggcaccgauc aggcuccacc aucggcaaag cauucgaagc aaccguacgc     1320

ggcgccaaac guauggccgu gcuaggcgac acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5195
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 1 opt

<400>  5195
gggagaaagc uuaccauguc acaaaaaguc auauacuuag ucaugauacu acucauugcc       60

cccgcauacu ccauacgaug caucggcguc uccaacagag acuucguaga aggcaugagc      120

ggcggcacau gggucgacgu aguccuggaa cacggcggcu gcguaaccgu gauggcccaa      180

gacaaaccaa cuguagacau agaacucguc acaaccaccg uaagcaacau ggccgaaauc      240

cgaagcuacu gcuacgaagc cagcauaagc gacauggcgu ccgacagccg cugcccaacc      300

caaggcgaag ccuaccuaga caaacagagc gacacacaau acgucugcaa acgcacacua      360

guagacagag gcuggggaaa cggcugcgga cuauucggaa aaggaagccu aguaacgugc      420

gcgaaauucg ccugcagcaa aaaaaugaca ggaaaaucaa uccagcccga gaaccuagaa      480

uacagaauua ugcuaagcgu acacgggucc caacacucag gaaugauagu aaacgacacu      540

ggccacgaaa ccgacgaaaa cagagccaaa guagaaauaa cacccaacuc accccgagcc      600

gaagccacac uaggaggcuu cggcucacuc ggacucgacu gcgaaccaag aaccggacua      660

gacuucucgg accucuacua ccucacgaug aauaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccccu cccuuggcac gcuggcgcag acaccggaac accacacugg      780

aacaacaagg aagcacucgu cgaauucaaa gacgcccacg ccaaacgcca aaccguaguc      840

guacucggau cccaagaagg ggcaguacac acagcccugg ccggagcacu cgaagcagaa      900

auggacggcg ccaaaggacg acuaucuagu ggccaccuga aaugucgccu aaaaauggac      960

aaacuccgac ucaaaggcgu aagcuacagc cucugcaccg cagccuucac cuucaccaaa     1020

auaccagcag aaacacuaca cggcacggua accgucgaag uccaguacgc aggaacugac     1080

gggcccugca aagucccagc ccaaauggca gucgacaugc aaacccuuac ccccguagga     1140

cgccucauaa cagccaaccc agucaucacc gaaagcacag aaaacagcaa aaugaugcuc     1200

gaacucgacc cacccuucgg agacucauac auagucauug gcgugggcga aaaaaaaaua     1260

acucaccacu ggcaccgauc gggcucaacu auaggcaaag ccuucgaagc gaccguccgc     1320

ggcgccaaac gcauggccgu acucggcgac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5196
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 1 opt

<400>  5196
gggagaaagc uuaccaugag ccaaaaagua auauaccucg ucaugauccu gcucauagcc       60

cccgcuuacu ccauacgcug caucggcguc agcaacaggg acuucguaga aggcauguca      120

ggcggcacau ggguagacgu aguccuagaa cacgggggau gcgucaccgu aauggcccaa      180

gacaaaccaa caguagacau cgaacucguc acgacgaccg ucuccaacau ggccgaagua      240

cgcagcuacu gcuacgaagc aagcauauca gacauggcau ccgacucccg cugccccacc      300

caaggcgaag ccuaccucga caaacaaucc gacacccaau acguaugcaa acgaacccua      360

guagaccgag gcuggggaaa cggcugcgga cuauucggaa aaggcucacu cguaacaugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggaaaaucca uccaacccga aaaccucgaa      480

uaccgcauaa ugcucuccgu ccacggaagc caacacucgg gcaugaucgu aaacgacacc      540

ggccacgaaa ccgacgaaaa ccgcgcaaaa guagaaauaa cacccaacag ccccagagca      600

gaagcaaccc ucggcggcuu uggaucccuc ggacuagacu gcgaaccccg aaccggccuc      660

gacuucagcg aucuauacua ccucacaaug aacaacaaac acuggcuagu ccacaaagaa      720

ugguuccacg acauaccucu gcccuggcac gccggugcag acacaggcac cccgcacugg      780

aacaacaaag aagcccuagu cgaguucaaa gacgcccacg ccaaacgcca aacagucguc      840

guccuaggca gccaagaagg cgcaguccac accgcccuag caggagcccu agaagcagaa      900

auggacggag caaaaggaag auuaucaucc ggccaccuga aaugucgccu aaaaauggac      960

aaacuccgac ucaaaggggu cagcuacagc cucugcacgg ccgcauucac auucacaaaa     1020

aucccagcag aaacacucca cggaaccguc acaguagaag uccaauacgc aggcaccgac     1080

ggccccugca aaguaccagc acaaauggcc guagacaugc aaacccuaac ccccgucgga     1140

cgccuuauca ccgcaaaccc aguaaucacg gaaucaaccg aaaacagcaa aaugaugcua     1200

gaacucgacc cgcccuucgg cgacagcuac auaguuauag gcguaggaga aaagaaaaua     1260

acccaccacu ggcaucgcag cgguucaacc aucggaaaag ccuucgaggc caccguacgc     1320

ggcgcacgcc gcauggccgu acucggcgac acggccuggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5197
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 1 opt

<400>  5197
gggagaaagc uuaccaugag ccaaaaagua auauaccuag ucaugauacu acucaucgca       60

cccgccuacu caauccgcug cauaggcguc ucaaaccgag acuucgucga aggcaugagc      120

ggaggaacgu gggucgacgu aguacucgaa cacggcggau gcgugaccgu uauggcacaa      180

gacaaaccaa ccgucgacau agaacucguc acaacaacgg ucucaaacau ggccgaaguc      240

cgcucauacu gcuacgaagc auccauaagc gacaugguau cugacucacg cugcccaacc      300

cagggagaag cguaccuaga caaacaaagc gacacccaau acgucugcaa aagaacccua      360

guggaccgcg gauggggaaa cggcugcggc cuauucggca aaggaagccu aguaacaugc      420

gccaaauucg ccugcagcaa gaaaaugacc ggcaaaucaa uacagcccga aaaccuagaa      480

uacagaauaa ugcuaagcgu ccacggcuca caacacuccg gaaugaucgu aaacgacacc      540

ggacaugaaa ccgacgaaaa ccgagcaaaa gucgaaauca ccccaaacuc acccagagcc      600

gaagccaccc uaggaggauu cggcagccua ggccuagacu gcgaaccacg aaccggacuc      660

gacuucuccg accucuacua ccuaacaaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acaucccccu ccccuggcac gcaggagcag acaccggcac accccacugg      780

aacaacaaag aagcgcucgu cgaguucaaa gacgcacacg ccaaacgcca aaccgucguc      840

guacuaggaa gucaagaagg cgccguacac accgcacucg caggcgcacu agaagcagaa      900

auggacggag ccaaaggccg acucagcagc ggacaccuca aaugccgacu aaaaauggac      960

aagcuacgcc ucaaaggagu cuccuacagc cuaugcacag cagcguuuac auucacgaaa     1020

aucccagcag aaacccucca cggcacaguc acggucgaag uacaauacgc aggcacagac     1080

ggacccugca aaguccccgc gcaaauggcc guagacaugc aaacccugac cccaguaggc     1140

cgccuuauca ccgccaaccc agucaucacc gaauccacag aaaacuccaa aaugaugcuc     1200

gaacuagauc ccccguucgg agacuccuac auaguaauag gcguaggaga aaagaaaauc     1260

acccaccacu ggcacagauc gggcuccaca aucggcaaag cauucgaagc aaccguccga     1320

ggcgccaaac gcauggccgu acucggagac acggcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5198
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 1 opt

<400>  5198
gggagaaagc uuaccaugag ccaaaaagua auauaccucg ucaugauccu ucucaucgca       60

ccagcauauu ccauacgcug cauuggagua ucaaacagag acuucgucga aggaaugagc      120

ggaggcacgu ggguggacgu gguccuagaa cacgggggcu gcguaaccgu aauggcucaa      180

gacaaaccga caguagacau cgagcuaguc accaccaccg ucucaaacau ggcagaaguc      240

agaaguuacu gcuacgaagc cagcauaagc gacauggcaa gcgacagcag augccccacc      300

caaggcgagg cauaccucga caaacaaagc gacacccaau acguaugcaa acgcacacuc      360

guagaccgcg gcuggggcaa cggcugcgga cuauucggca aaggaagccu cgucacaugc      420

gccaaauucg caugcucaaa gaaaaugacc ggcaaaagca uacagcccga gaaccuggaa      480

uaccgaauaa ugcucagcgu acacggauca caacacucag gcaugaucgu gaacgacaca      540

gggcaugaaa ccgacgaaaa ccgcgccaaa guagaaauca ccccgaacuc cccaagagcc      600

gaagccacac ucggcggcuu cggcucacua ggacucgacu gcgagccaag aaccggccua      660

gacuucucag accuuuacua ccucacaaug aauaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccccu ccccuggcac gcaggcgcag auacaggaac gccccacugg      780

aacaacaagg aagcacuugu agaguucaaa gacgcacacg ccaaacgaca aaccguaguc      840

guacucggaa gccaagaagg agccgugcac accgcccuag caggagcccu cgaagccgaa      900

auggacggag ccaaaggcag acuaucaagc ggacaccuaa aaugccgccu aaaaauggac      960

aaacuacgac ucaagggcgu auccuacagc cuaugcaccg cugcauuuac cuuuacaaaa     1020

auacccgccg aaacacuaca cggcacagua acagucgaag uccaguacgc aggcacagac     1080

gggcccugca aaguaccagc acaaauggca guagacaugc aaacgcuaac gcccguagga     1140

cgccucauca cagccaaccc agucaucacc gaaagcauag aaaacagcaa aaugaugcua     1200

gaacuagauc ccccauucgg agacucauac auagucaucg gcguaggaga aaagaaaauc     1260

acgcaccacu ggcaccgcuc aggaucaacc aucggcaaag ccuuugaagc aacagugcga     1320

ggcgccaaac gcauggccgu acucggagac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5199
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 1 opt

<400>  5199
gggagaaagc uuaccauguc gcaaaaagua auauaccuag ucaugauacu gcucauagca       60

ccagccuaca guauacgaug caucggcguc agcaacaggg acuucgucga aggcaugagc      120

ggaggcacau ggguugacgu gguacuagaa cacggcggau gcguaaccgu gauggcacaa      180

gauaaaccaa cgguagacau cgaacuggua acaacaacag uaagcaacau ggccgaagua      240

aggagcuacu gcuacgaagc aagcauaagc gacauggcaa gcgacuccag augcccaaca      300

caaggcgaag ccuaccucga caaacaaagc gacacccaau acgucugcaa acgcacacuc      360

guagauagag gcuggggaaa uggcugcggc cuauucggaa aaggaagccu cgucacgugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggcaaaucga uccaaccaga aaacuuagaa      480

uaucgaauca ugcugagcgu acacgggagc caacacuccg gaaugauagu gaacgacaca      540

ggacacgaaa ccgacgagaa cagggcaaaa guagaaauca caccaaacuc accccgggcc      600

gaagccacac ucggaggauu cggaagccuc ggacuagacu gcgaaccaag aacaggacuc      660

gacuucagug accuauacua ccucacaaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acaucccacu gccauggcac gcgggcgcag acacaggcac accccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacacg ccaaacgcca aaccguaguc      840

guacuaggaa gccaagaagg ggccguacac acagcccugg ccggggcacu cgaagcagaa      900

auggacggag caaaaggcag gcucucguca ggacaucuaa aaugccgccu aaaaauggac      960

aaacucaggc ucaaaggcgu gagcuacagc cuguguacag cagcauucac auucacaaaa     1020

auacccgcag aaacacucca cggcacaguc acgguagaag uacaauacgc aggcacagac     1080

ggcccaugca aaguacccgc gcagauggcc gucgacaugc aaacacuaac acccguagga     1140

cgacuaauaa ccgcaaaccc ggucauaacc gaaagcacgg aaaauagcaa aaugaugcuc     1200

gaacuagacc caccauucgg cgauucauac aucgucaucg gcauaggcga aaagaaaauc     1260

acacaccacu ggcaccgcuc aggcucaaca auaggcaaag ccuuugaagc aaccguacgc     1320

ggggcaaaac gcauggcagu gcuaggggac acagcauggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5200
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 1 opt

<400>  5200
gggagaaagc uuaccauguc acagaaaguc aucuaccuag ucaugauccu ccuaaucgca       60

cucgccuacu caauacgcug cauaggaguc uccaaccgag acuucguaga aggaaugucc      120

ggcggaaccu ggguagacgu aguccucgaa cacgggggcu gcguaacagu cauggcccaa      180

gacaaaccca ccgucgacau agaacucgua accaccaccg ucucaaacau ggccgaaguc      240

cgaucguacu gcuacgaagc cagcaucucc gacauggccu ccgacucccg augcccaacc      300

cagggagaag ccuaccuaga caaacaaagc gacacacaau acguaugcaa acgaacacuc      360

gucgaccgcg gcuggggaaa cgguugcgga cuauucggca aaggaagccu agucacaugc      420

gcaaaauucg caugcucaaa gaaaaugacc ggcaaaucca uccaaccaga aaaccucgaa      480

uaccgaauaa ugcucagcgu ccacggcuca caacacagcg gaaugauagu aaacgacacc      540

ggacacgaaa ccgacgaaaa ccgcgcaaaa gucgaaauca cccccaacuc cccacgcgcc      600

gaagccaccc uaggaggauu cggaucccuc ggacuagacu gcgaacccag aaccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaaac acugguuagu ccacaaagaa      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acacaggaac cccgcacugg      780

aacaacaaag aagcccucgu cgaguucaaa gacgcgcacg ccaaacgaca aaccgucguc      840

guacuagggu cccaagaagg agccguacac acagcacuag ccggcgcccu ugaagcagaa      900

auggacggcg cuaaaggccg acucagcucc ggacaccuca aaugcagacu uaaaauggac      960

aaacuccgcc ucaaaggcgu cagcuacagc cucugcaccg ccgcauucac auucacaaaa     1020

auacccgccg aaacccucca cggcaccguc acaguagaag uccaauacgc cggaaccgac     1080

ggccccugca aaguaccagc ccaaauggca gucgacaugc aaacacuaac cccagucgga     1140

cgacuuauca cggcaaaccc cgucaucaca gaaucaaccg aaaacuccaa aaugaugcuc     1200

gaacucgacc caccuuucgg cgacuccuac aucguaaucg gaguaggcga aaagaaaauc     1260

acacaccacu ggcaccgauc cggauccacg aucggcaaag ccuucgaagc aacaguccgc     1320

ggagcaaaaa gaauggccgu acucggagau accgccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5201
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 1 opt

<400>  5201
gggagaaagc uuaccaugag ccaaaaaguc auauaccuag ucaugauccu ccucauagcc       60

ccagcauacu ccauacgcug caucggagua agcaaccgag acuucgucga aggaauguca      120

ggcggaaccu gggucgacgu cguacuagaa cacggcggau gcguaacagu aauggcccaa      180

gacaaaccaa caguagacau cgaacucgua acaaccacag ucagcaacau ggcagaagua      240

agauccuacu gcuacgaagc cucaauaagc gacauggcau ccgacuccag gugcccaaca      300

caaggcgaag ccuaccucga caaacaaagc gacacacaau acgucugcaa aagaacacuu      360

guagacagag gauggggcaa cggaugcggc cucuucggaa aaggaagccu cguaaccugc      420

gccaaauucg ccugcagcaa aaagaugaca ggaaaaucga uccaacccga aaaccuagaa      480

uaccgcauca ugcucuccgu ccacggcagc cagcacucag gcaugauagu caacgacaca      540

ggacacgaaa cagacgaaaa ccgagccaag guagaaauaa ccccaaacag cccccgagcc      600

gaagcaaccc ucggaggauu cggaucacua ggacuagacu gcgaaccccg aaccggccuc      660

gacuucuccg accuauacua ccuaaccaug aacaacaaac acuggcucgu ccauaaggaa      720

ugguuccacg acauaccccu gcccuggcac gcgggcgcag acacgggcac accccacugg      780

aacaacaaag aagcccucgu cgaguucaaa gacgcccacg caaaacgcca gaccguaguc      840

guacuaggca cccaagaagg agccguccac accgcccugg ccggagcccu cgaagccgaa      900

auggacggag caaaagggcg acucagcagc ggccaccuaa aaugccgccu aaaaauggac      960

aaacuaagac uaaaaggcgu aucauacagc cuaugcacag cagcauucac cuucaccaaa     1020

aucccugcag aaacacucca cggcaccguc acggucgaag uccaguacgc cggcacagac     1080

ggacccugca aagucccagc ccaaauggcc gucgacaugc aaacccuaac acccgucgga     1140

agacuaauaa ccgccaaccc cguaaucacc gaaagcacag aaaauucaaa aaugaugcuc     1200

gaacuagauc ccccguucgg agacuccuac auaguuaucg gcguaggcga gaaaaagauc     1260

acacaccacu ggcacagauc cggcagcaca auaggaaagg ccuucgaagc aacaguccgc     1320

ggagcaaaac gaauggcagu ccucggagac acagccuggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5202
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 1 opt

<400>  5202
gggagaaagc uuaccauguc gcaaaaaguc aucuaccuag ucaugauccu ccuaauagcc       60

ccggccuaca guauacgaug caucggcguc agcaacaggg acuucgucga aggcaugagc      120

gggggcacgu ggguagacgu aguuuuagaa cacggcggau gcguaaccgu gauggcacaa      180

gacaagccaa ccguagacau agagcuaguc acaaccacag uuucaaacau ggcagaaguc      240

aggagcuacu gcuacgaagc gagcauaagc gacauggcga gcgacagcag augcccaacu      300

caaggcgaag ccuaccuuga caaacaaagc gacacccagu acguaugcaa aagaacccua      360

gucgaccgag gaugggggaa cgguugcgga cuauucggaa aagggagccu agucacaugc      420

gcgaaauucg ccugcagcaa aaagaugaca ggaaaaagca uccaacccga gaaccuagaa      480

uaucgaauaa ugcucagcgu acacgggagc caacacucag gaaugauagu caacgacaca      540

gggcacgaaa ccgacgaaaa ccgagcgaaa guggagauaa cuccaaacuc acccagagcu      600

gaggcaacac uaggaggcuu cgggucacuu ggacuagacu gcgaacccag aacaggccuc      660

gacuucucgg accuauacua ccuaacaaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauaccacu accuuggcac gcaggagcag acacagguac accacacugg      780

aacaacaagg aagcacucgu agaguucaaa gacgcacaug ccaaacgaca gaccguaguc      840

guacucggga gccaagaagg ggccguacac acggcccugg caggagcacu agaagcagaa      900

auggacggag ccaagggccg acucagcagc ggccaccuca agugcagauu gaaaauggac      960

aagcuacgac ucaaaggcgu aagcuacagu cuaugcaccg ccgcauucac auucacaaaa     1020

aucccagcag aaacgcuaca cggcacaguc accgucgaag uucaguacgc cggaacagac     1080

ggaccaugca aagugcucgc ccagauggca gucgacaugc aaacgcuaac gcccguaggu     1140

aggcucauaa ccgcgaaccc gguaaucaca gaaagcacag aaaacagcaa aaugaugcuc     1200

gagcucgacc cacccuucgg agacagcuac aucgucauag gggugggaga aaagaaaauc     1260

acacaccacu ggcaccgcag cgguucaacc aucggcaaag ccuucgaagc gaccguacga     1320

ggagcuaaaa gaauggccgu acucggagac acagccuggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5203
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 1 opt

<400>  5203
gggagaaagc uuaccaugag ccagaaaguc aucuaccuag ucaugauccu gcucauagca       60

ccagcauaca gcauaagaug uaucggaguc uccaacagag acuucgucga aggaaugagc      120

ggaggaaccu ggguagacgu agucuuagaa cacgguggcu gcgucaccgu gauggcacaa      180

gacaaaccga caguagacau agaacucguc acaacaacag uaagcaacau ggcagaagua      240

cgaagcuacu gcuacgaagc guccauaagc gacauggcca gcgacagcag augcccaaca      300

caaggcgaag cauaccuaga caaacaaucc gacacacagu acgucugcaa acgcacccua      360

guagacagag gguggggcaa cggaugcgga cuauucggga aagggagccu agucacaugc      420

gccaaauucg ccugcucaaa gaaaaugaca ggaaaaagca uacaaccaga aaaccuagaa      480

uaccgaauaa ugcuaagcgu acacggaagc caacacagcg gcaugaucgu aaacgacacc      540

ggacacgaaa cagacgaaaa ccgagcaaaa gucgaaauaa cacccaacag cccccgagcc      600

gaggccacac uaggcgguuu cggaagccuc ggacucgacu gcgaaccacg cacagggcua      660

gacuucuccg accucuauua ccuaacaaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccccu acccuggcac gcaggcgcgg acacaggaac cccgcacugg      780

aacaacaaag aagcccuagu cgaguucaaa gaagcccacg ccaaacgaca gacaguaguc      840

guccuaggaa gccaagaagg agccguccac acagcccuag caggagcacu cgaagcagaa      900

auggacgggg ccaaaggacg ccuaagcucc ggacaucuaa aaugccgccu aaaaauggac      960

aaacucagac uaaaaggagu cagcuacagc cuaugcacag ccgccuucac cuucaccaaa     1020

auaccggcag aaacacucca cggcacaguc accgucgaag uccaauacgc aggcaccgac     1080

ggaccaugca aaguacccgc acaaauggcc guagacaugc aaacacuaac cccaguggga     1140

cgacucauaa cugccaaccc agucaucacc gaaucaaccg aaaacagcaa aaugaugcua     1200

gagcucgacc caccauucgg agacagcuac auagucaucg gcgucggcga gaagaaaauu     1260

acgcaucacu ggcacagaag cggcucgacc auaggcaaag ccuucgaagc aacaguacgc     1320

ggagcaaaac gaauggccgu ccucggagac acagccuggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5204
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 1 opt

<400>  5204
gggagaaagc uuaccauguc gcaaaaaguc aucuaccuag uaaugauccu gcuaauagca       60

ccagccuaca guaucagaug cauaggaguc ucgaacaggg acuucguaga gggaauguca      120

gggggcacgu ggguagacgu gguacuagaa cacggcggau gcgugaccgu gauggcgcaa      180

gacaaaccga ccguagacau agaacucguc acgacaacgg ucucaaacau ggccgagguc      240

aggagcuacu gcuacgaagc gaguauaagc gacauggcaa gcgacagcag augcccgaca      300

cagggcgagg ccuaccucga caaacagagc gacacccaau acgugugcaa acgcacccua      360

guggaccgag gcuggggcaa cggcuguggc cuguucggga aggggagccu cgucacaugc      420

gcaaaauucg ccugcagcaa gaaaaugaca ggaaaaagca uccagcccga aaaccuggaa      480

uacagaauaa uguuaagcgu gcacggaagc cagcacucag gcaugaucgu aaacgacaca      540

ggccacgaga ccgacgaaaa ccgcgcaaaa guggaaauaa ccccgaacuc gccgagggca      600

gaagccgccc uagggggauu cggcucgcua gggcuagacu gcgagccaag aacaggccug      660

gacuucagcg accuauacua ccuaacgaug aacaacaaac acuggcuagu gcacaaggag      720

ugguuccacg acauacccuu gcccuggcac gcgggagccg acacaggaac gccucacugg      780

aauaacaagg aagcccuagu cgaguucaaa gacgcacacg caaaacgaca gaccguagua      840

gugcuaggga gccaggaagg ggcagugcac acggcucugg ccggagcacu ggaagcggaa      900

auggacgggg ccaaaggcag gcucucaucc gggcaccuga aaugccgccu gaagauggac      960

aaacuaagac ucaaaggcgu cagcuauagc cuaugcacgg ccgcguucac guucacgaaa     1020

auaccagccg aaacgcuaca cggaacgguc acgguagaag uccaguacgc gggcacggac     1080

gggccaugca aagugccggc gcaaauggcc guagacaugc aaacgcucac gccaguggga     1140

aggcuaauaa ccgcgaaccc aguaauaaca gaaagcacag aaaauagcaa gaugaugcuc     1200

gagcucgauc ccccguucgg ggacucauac auagucauag gcgugggcga gaaaaagaua     1260

acgcaccacu ggcacagauc gggaucgacc auaggcaaag ccuuugaagc gaccguacgc     1320

ggggcaaaac gcauggcagu acucggggac acggcauggg acuuugguuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5205
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 1 opt

<400>  5205
gggagaaagc uuaccauguc ccagaaaguc auauaccucg uaaugauccu acuaaucgca       60

ccagcauaca gcauaagaug cauaggcguu uccaaccgag acuucguaga aggcaugagc      120

ggcggcacau ggguagacgu cguccucgaa cacggaggau gcguaaccgu cauggcccaa      180

gacaaaccca cagucgacau cgaacugguc ucuacaacag ucuccaacau ggccgaaguc      240

cgaagcuacu gcuacgaagc aaccauaucg gacaucgcau ccgacagccg cugcccgaca      300

caaggcgaag ccuaccucga caagcaaagc gacacacaau acgucugcaa acgaacgcuc      360

gucgaccgag gcugggguaa cggcugcggc cucuucggca aagggucccu cguaacaugc      420

gccaaauucg ccugcucaaa gaaaaugaca ggcaaaucca uccaaccaga aaaccucgaa      480

uacagaauca ugcuauccgu acacggcagc caacacuccg gcaugauugu aaacgacaca      540

ggccacgaaa ccgacgaaaa ccgagcaaaa guagaaauca ccccaaacag cccccgagca      600

gaagccaccc ucggcggauu cggaagccuc ggccucgacu gcgaacccag aaccggacuc      660

gacuucagcg accucuacua ccucaccaug aauaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acaucccccu acccuggcac gcaggcgccg acaccggaac accccacugg      780

aacaacaaag aagcacucgu cgaguucaaa gacgcccacg ccaaacgcca gaccgcaguc      840

guccucggau cacaggaagg cgccguccau acagcccuag ccggcgcccu agaagcagaa      900

auggacggcg cgaaaggccg ccucagcagc ggccaccuaa aaugccgccu caaaauggac      960

aaacuacgcc uuaaaggcgu cagcuauagc cucugcacag cugcauucac cuucacaaaa     1020

auccccgcag aaacccuaca cggcacaguc accgucgaag uacaauacgc cggcacagau     1080

ggcccaugca aagugcccgc ccaaauggcc guggacaugc aaacccucac cccagucggc     1140

agacucauaa ccgccaaccc aguaaucacc gaaucaaccg aaaacuccaa aaugaugcuc     1200

gaacucgauc ccccauuugg agacuccuac auagucaucg gcguaggcga aaagaaaauc     1260

acccaccacu ggcaccgcag cggauccacc auaggcaaag ccuucgaagc cacaguccga     1320

ggagcaaaaa gaauggccgu gcucggagac acagcauggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5206
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 1 opt

<400>  5206
gggagaaagc uuaccauguc acaaaaaguc auauaccuag ucaugauccu acuaauagca       60

ccagcguaua gcauacgcug caucggagua agcaacaggg acuucgucga gggcaugagc      120

ggaggcacau ggguggacgu cguacuggaa cacggaggau gcguaacagu gauggcacaa      180

gacaaaccaa ccguagacau agagcuagua acaacaacag ucucgaacau ggccgaaguc      240

agaucauacu gcuacgaagc cagcauaagc gacauggcau ccgacuccag gugcccaacc      300

cagggcgaag cauaccucga caaacaaagc gacacacagu acguaugcaa aagaacccua      360

guggacagag gauggggcaa cggaugcgga cuauucggaa aagggagccu cgucacaugc      420

gcaaaauucg ccugcagcaa gaaaaugaca gggaaaucaa uacagccaga aaaccuagaa      480

uaccgaauaa ugcucagcgu gcacggaagc caacacucag gcaugaucgu aaacgacacc      540

gggcacgaaa cagacgaaaa ccgcgcaaaa gucgaaauaa caccaaacuc acccagagcc      600

gaggccacac uugggggauu cggcagccuc gguuuagacu gcgaaccaag gaccgggcuc      660

gacuucucag accuguacua ccucacaaug aauaacaaac acuggcucgu acacaaagaa      720

ugguuccaug acaucccccu acccuggcac gccggcgcag acacaggcac gccacacugg      780

aacaacaaag aagcgcucgu ggaguucaaa gacgcccacg ccaaacgcca aaccguagug      840

gugcuaggga gccaggaagg agcagugcac acagccuuag ccggcgcccu ggaagcagaa      900

auggacggag caaaaggccg gcucagcagc ggacaccuca aaugccgacu gaaaauggac      960

aaacuacgcc uaaaaggagu gagcuacagc cucugcacag ccgcguucac auucagcaag     1020

auacccgcgg aaacacuaca cggaacagua acagaagaag uccaauacgc aggcaccgac     1080

ggacccugca aggugcccgc acaaauggcc guagacaugc aaacacucac accgguuggc     1140

agacucauca ccgcaaaccc cgugauaacc gaaagcacag aaaacucaaa aaugaugcuc     1200

gaacucgacc cgccauucgg agacucguac auaguaauag gaguaggcga aaagaaaauc     1260

acgcaccacu ggcaccgcag cggaucaaca aucggcaaag ccuucgaagc gaccgucaga     1320

ggagcaaaac gcauggcggu acugggagac acagcauggg acuucggauc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5207
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 1 opt

<400>  5207
gggagaaagc uuaccauguc ccaaaaagua auauaccuag uaaugauccu ccuaauagca       60

cccgcauaca gcauccgcug cauagggguc ucgaacaggg acuucguaga aggcaugagc      120

ggaggaacau ggguagacgu agucuuagaa cacggcggcu gcgucaccgu aauggcccaa      180

gacaaaccaa ccguagacau agaacugguc accaccacgg uaucgaacau ggcagaaguc      240

cgaagcuacu gcuacgaagc cagcaucagc gacauggccu cagacagccg augccccacc      300

caaggcgaag ccuaccucga caaacaauca gacacccaau acguaugcaa acgcacccua      360

guagaccgag gcuggggcaa cggcugcggc cuguucggaa aagggagccu ggucacaugc      420

gccaaauucg ccugcuccaa gaaaaugaca ggaaaaucaa uccaacccga aaaccuagaa      480

uaucgcauca ugcuauccgu acacggaucc caacacuccg gaaugauagu aaacgacaca      540

ggacacgaaa ccgacgaaaa ccgcgccaaa guagaaauaa ccccaaacuc ucccagagcc      600

gaagccacac ucggaggauu cggcucccua ggccuagacu gcgaaccccg gaccgggcuc      660

gacuucuccg accucuacua ccucaccaug aacaacaaac acuggcucgu ccauaaagaa      720

ugguuucacg acauaccccu cccauggcac gcaggcgcag acaccgguac accacacugg      780

aacaacaagg aagcgcuagu agaguucaaa gacgcccacg ccaaacgcca aaccguaguc      840

gugcuaggca gccaggaagg agccguacac acagcacugg ccggagcucu cgaagcagaa      900

auggacggag ccaaaggccg gcuaucaagc ggacaccuaa aaugccgccu aaaaauggac      960

aagcuaagac ucaaaggcgu gagcuacagc cuaugcacag ccgcauucac guucaccaaa     1020

auaccugcag aaacccuaca cggaacagua acagucgaac uccaauacgc aggcacggac     1080

ggacccugca aagugcccgc ccaaauggcc guggacaugc aaacgcucac acccguagga     1140

aggcucauua cagcaaaccc agucaucacc gaaagcacag aaaacucaaa aaugaugcua     1200

gaacuugauc ccccauucgg agacucguac auagucaucg gcguagggga gaaaaagauc     1260

acacaccacu ggcaccgauc aggcucaaca aucggcaagg ccuucgaagc caccguccga     1320

ggagcaaaac gaauggccgu acuaggggac acagccuggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5208
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 1 opt

<400>  5208
gggagaaagc uuaccaugag ccaaaaaguc auauaccugg ucaugauccu ccuaauugca       60

ccagccuaca gcauacguug caucggcauc uccaacagag acuuugucga aggcaugagc      120

ggagggacgu ggguagacgu gguacuagaa cacggaggcu guguaaccgu aauggcccaa      180

gacaaaccaa ccgucgacau cgagcuaguc acuacaacag ucagcaacau ggcagaaguc      240

cgaagcuacu gcuacgaagc cagcauaagc gauauggcga gcgacagcag augcccaacc      300

caaggugaag cauaccuaga caaacaaagc gacacccagu acgugugcaa acgcacacug      360

guagaccggg gcuggggcaa cggcugcggc cuguucggca aaggaucccu agucacaugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggaaaaucua uccaaccaga aaaccuagaa      480

uaucgcauaa ugcuaagcgu acacggaagc cagcacuccg gaaugaucgu aaacgacacc      540

ggacacgaaa ccgacgaaaa ccgagccaaa guagaaauca cccccaacuc gcccagagca      600

gaagcaaccc uuggaggcuu cggaauccua ggacucgacu gcgaacccag gaccggacua      660

gacuucagug accuguacua ccuaacaaug aacaacaaac acuggcucgu ccacaaggaa      720

ugguuccacg acauaccccu cccuuggcac gcaggcgcag acacaggaac gccacacugg      780

aacaacaagg aagcacucgu cgaguucaaa gacgcccacg ccaaacguca aaccgucgua      840

guccucggcu cacaagaagg agccguacac acagcccugg caggagcccu cgaagcagaa      900

auggacggag caaaaggacg ccuaagcagc gggcaccuaa aaugccgccu aaaaauggac      960

aaacuccgac ucaaaggcgu aagcuacagc cuaugcaccg ccgcauucac cuucacaaaa     1020

auacccgcag aaacccucca cggcaccguc accgucgaag uccaauacgc aggaaccgac     1080

ggaccaugca aaguccccgc acaaauggca guagacaugc aaacccucac accaguagga     1140

agacucauca ccgcaaaccc agucaucacc gagagcaccg agaacucaaa aaugaugcuc     1200

gaacucgacc cacccuucgg cgacucuuau auagucaucg ggguaggaga aaagaaaauc     1260

acacaccacu ggcaccgauc gggcuccacc auaggaaaag ccuucgaagc aaccguccgc     1320

ggagccaaac gcauggcugu gcucggagac acggcuuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5209
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 1 opt

<400>  5209
gggagaaagc uuaccaugag ccaaaaagua auauaccucg ucaugauacu acuaauagcc       60

ccggcauaca guaucagaug caucgggguc agcaacagag acuucgucga aggcaugagu      120

ggaggcacgu ggguagacgu aguacuagaa cacggaggau gcgucacagu cauggcacaa      180

gacaaaccga ccguagacau agagcuagua acaaccaccg ucagcaacau ggccgaaguc      240

agaagcuacu gcuacgaagc caguauaucc gacauggcca gcgacucgag gugccccacc      300

caaggcgaag ccuaccuuga caaacaaagc gacacacaau acguaugcaa aagaacccua      360

gucgaccgag gcuggggaaa cggaugcggc cucuucggaa agggaagccu agucacaugc      420

gccaaauuug caugcagcaa gaaaaugaca ggaaaaagca uacaacccga aaaccuagaa      480

uaccgcauca ugcuaagcgu ccacggaagc cagcauagcg gcaugauagu aaacgacaca      540

gggcacgaaa ccgacgaaaa ccgagccaaa guagaaauca caccaaacag ccccagagcc      600

gaagccacgc ucggaggcuu cggaagccua ggacucgacu gcgaacccag aaccggacuc      660

gacuucagug accucuacua ccuaacaaug aauaacaaac acuggcuggu ccacaaagaa      720

ugguuccacg acaucccacu acccuggcac gcaggagcgg acacgggcac accacacugg      780

aacaacaaag aagcucucgu agaauuuaaa gacgcccacg caaaacgcca gaccguaguc      840

guccuaggaa gccaagaagg agccguacac accgcccugg caggagcgcu ggaagcggaa      900

auggacggag uaaaaggaag gcuaagcagc ggccaccuca aaugccgccu aaaaauggac      960

aaacuacgcc uaaagggcgu aagcuacagc cuaugcacag ccgcguucac auucacaaag     1020

auaccagccg aaacgcucca cgggacaguc acaguagaag uccaauacgc aggaacagac     1080

ggaccaugca aagugccagc acaaauggcc gucgacaugc aaacccuaac ccccguaggc     1140

cggcuaauaa cagcaaaccc ugucauaacc gaaucgacag aaaacuccaa gaugaugcua     1200

gaacucgauc ccccauucgg cgacaguuac auugucauag gagugggcga aaagaaaaua     1260

acacaccacu ggcaucgaag cggaucaacc aucggcaaag ccuucgaagc caccguacga     1320

ggagccaaac gcauggcagu gcuaggcgac acagcauggg acuucggcag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5210
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 1 opt

<400>  5210
gggagaaagc uuaccaugag ccaaaaagua auauaccuag ucaugauccu acucauagcc       60

ccagcauaca gcauacgcug cauaggagug uccaaccgag acuucgucga aggcauguca      120

ggcggcacgu ggguagacgu aguacuagaa cacggcgggu gcguaacagu gauggcccaa      180

gacaaaccca cagucgacau cgagcucguc acaaccacag ucuccaacau ggccgaaguc      240

agaucauauu gcuacgaagc aagcaucucc gacauggcaa gcgacagcag augcccaacc      300

caaggagaag ccuaccuuga caaacaauca gacacccaau acgucugcaa acgcacccua      360

gucgaccggg gauggggcaa cggaugcggg cuauucggaa aaggaucccu aguuacaugu      420

gccaaauucg caugcucaaa gaaaaugacc gggaaaucaa uacaacccga aaaccuagaa      480

uaccgcauca ugcugagcgu acacggcagc caacauucag gcaugaucgu aaacgacacc      540

ggacacgaaa cagaugaaaa ccgcgccaaa guggaaauaa cuccaaacuc ccccagagcc      600

gaagccacac ucggaggcuu cggcucgcuc ggacucgacu gcgaacccag aacaggccuu      660

gacuucucag accuauauua ccuuacaaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg auauaccccu acccuggcac gcaggcgcag acaccggcac accccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacacg caaaacgcca aaccgucgua      840

guccucggau cacaagaagg cgccguacac acggcccuug ccggcgcacu agaagcagag      900

auggacggcg ccaaaggccg acuaucaagc gggcaccuga aaugcagacu caaaauggac      960

aaacuccgac uaaagggcgu guccuacuca cuaugcacag cagcauucac auucacaaag     1020

auacccgcag aaacccuaca cggaacggua acagucgaag uacaauacgc cggcgcagau     1080

ggaccaugca aaguacccgc gcaaauggcc guagacaugc aaacacucac gcccgucgga     1140

cgacucauaa cugcgaaccc cgucauaaca gaaagcacag aaaacuccaa aaugaugcua     1200

gaacucgauc ccccauucgg cgacuccuau auagucaucg gcgucggaga gaagaaaaua     1260

acacaccacu ggcaccgcuc cggcuccacu aucggcaaag ccuucgaagc caccguacga     1320

ggagccaaac gaauggccgu ccucggagac accgcauggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5211
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 1 opt

<400>  5211
gggagaaagc uuaccauguc ccagaaaguc aucuaccugg uaaugauccu acuaauagcc       60

cccgcauaca gcauaagaug caucggagua uccaacagag acuucgucga aggaaugucc      120

ggcggcacau ggguagacgu agucuuggaa cacggaggcu gcguaaccgu cauggcacaa      180

gacaaaccca ccgucgacau cgaacucguc acgaccacag ucucgaauau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcauaagc gacauggcca gcgacagcag augcccaacc      300

caaggcgaag ccuacuuaga caaacagucc gacacccaau acguaugcaa acgcacccuc      360

guagaccgag gcuggggaaa cggcugcggc cuguucggaa aaggaagccu cguaaccugc      420

gcgaaauucg ccugcuccaa gaaaaugacc ggaaaaucaa uccagccuga aaaccucgaa      480

uaccgcauaa ugcucagcgu ccacggaagc cagcauagcg gcaugaucgu aaaugacaca      540

ggacacgaaa ccgacgaaaa ccgcgcaaaa guagaaauaa caccgaacuc ccccagggcc      600

gaggccacac ucggcggcuu cggcucgcuc ggccucgacu gcgaaccaag gaccggacua      660

gacuucagcg accuauacua ccucacaaug aacaacaaac acuggcuagu acacaaagaa      720

ugguuccacg acauaccccu cccauggcac acaggcgccg acaccggcac accccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcccaug ccaaacgcca aaccgugguc      840

guccucggau cacaggaagg cgccguucac accgcucuag caggagcccu agaagccgaa      900

auggacggag ccaagggacg acucucaagc ggacaccuca aaugcagacu caaaauggac      960

aagcuacgac uaaaaggagu aagcuauagc cugugcaccg cagcauucac cuucaccaaa     1020

auacccgcag aaacacucca cggcacggua accgucgaag uacaauacgc aggcaccgac     1080

ggcccaugca aaguacccgc ccaaauggcc gucgacaugc aaacacuaac ccccguaggc     1140

cgacucauaa cagcaaaccc agucauaacc gaaggcaccg aaaauagcaa gaugaugcuc     1200

gaacuggacc cgcccuucgg cgacucguac auagucauag gagugggcga gaaaaagauc     1260

acacaccacu ggcaccgcuc cggcucaacc auaggcaaag ccuucgaagc caccguccga     1320

ggggccaaac gcauggccgu gcucggagac acagccuggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5212
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 1 opt

<400>  5212
gggagaaagc uuaccauguc gcaaaaagua auauaccucg ucaugauccu ccucauagcc       60

cccgccuacu ccauacgcug cauaggcguc uccaaccggg acuucgucga aggaaugagu      120

gggggcaccu gggucgacgu aguccuagaa cacggcggau gcguaaccgc aauggcccag      180

gacaagccca ccgucgacau agaacucgua acgacaacag ucuccaacau ggccgaaguc      240

agauccuacu gcuacgaagc aagcaucuca gacauggcaa gcgacagccg augcccaacc      300

caaggcgaag ccuaccuaga caaacaaagc gacacccaau acguaugcaa acgaacccua      360

gucgaccgcg gcuggggcaa cgggugcggc cuauuuggga agggaagccu cgucacaugc      420

gcaaaauuug caugcagcaa aaagaugacc gggaaaagca uccaaccaga aaaucucgaa      480

uaccgcauaa ugcuauccgu ccacggcagc caacacagcg gcaugcuagu aaacgacacc      540

ggacacgaaa ccgacgaaaa ccgcgcaaaa guagaaauaa caccaaacuc cccacgagca      600

gaagcaacac ucggcggcuu cggaagccua ggccuagacu gcgaacccag aaccggacuc      660

gacuucagcg accucuacua ccucacaaug aacaacaaac acuggcuagc ccacaaggaa      720

ugguuccacg acaucccacu acccuggcac gcaggcgcag caacgggaac accacacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacaug cuaaacgaca aaccgucgua      840

guacuaggaa gccaagaagg cgcaguccac accgcccucg ccggagcacu agaagccgaa      900

auggacggag ccaaggggag acuuucaagc ggccaccuaa aaugucgccu aaaaauggac      960

aaacuacgcc ucaaaggcgu cagcuacagc cucugcacag ccgcauucac auucaccaaa     1020

auaccugcug aaacagucga cggaacagua acagucgaag gacaauacgg aggaacugac     1080

ggccccugca agguaccagc acaaauggcc gucgacaugc agacgcuaac gccaguagga     1140

cgacucauaa ccgccaaccc agucauaacc gaauccacag aaaacagcaa aaugaugcuc     1200

gaacuagacc cacccuucgg ggacuccuac auagucauag gagucggcga aaagaaaauc     1260

acccaccacu ggcaccgcag cggcucaacc auaggcaaag cauucgaagc gacaguacgc     1320

ggagcaaaac gcauggccgu acuaggagac acagccuggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5213
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 1 opt

<400>  5213
gggagaaagc uuaccauguc ccaaaaggug auauaccuag ucaugauccu ccuaauagcc       60

ccagccuaca gcauacggug caucggaguc uccaacagag acuucgucga aggcaugagc      120

ggaggaacau ggguagacgu aguccuagaa cacggcggau gcguaacagu gauggcgcaa      180

gacaaaccag ccguagacau agaacugguc acaacaacgg ucagcaacau ggcagaaguc      240

agaagcuacu gcuacgaagc aagcauaagc gacauggcga gcgacagcag augcccaacc      300

cagggcgaag cauaucucga caaacaauca gacacacagu acguaugcaa gagaacgcuc      360

guagaccgag gcuggggcaa cggaugcggc cuguucggaa aggggagccu cgucacgugc      420

gcgaaauucg cgugcagcaa gaaaaugacc gggaaaucaa uacagccaga gaaccuggaa      480

uaccgaauaa ugcucagcgu ccacggcagc caacacagcg gcaugaucgu caacgacaca      540

ggacaugaaa ccgacgaaaa ucgcgcaaaa gucgaaauca caccgaacuc gcccagagcc      600

gaagccacac ucgggggauu cgguagccua ggacucgacu gcgagcccag gacaggacuc      660

gacuucucgg accuguacua ccuaacaaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauaccccu acccuggcac gcaggcgcag acacaggcac accccacugg      780

aacaacaaag aagcccuagu ggaguucaaa gacgcacaug caaaacgaca aaccguagua      840

gugcuaggaa gccaggaagg agccguacac acagcccugg ccggagcauu ggaagccgaa      900

auggacggag caaaaggcag gcucuccagc gggcaccuga aaugccgccu gaaaauggac      960

aaacuacgac ucaaaggcgu guccuacagu cucugcaccg cagcguucac auucacaaaa     1020

auacccgcag aaacgcucca cggcacgguc acggucgaag uccaauacgc uggaaccgac     1080

ggacccugca aaguaccagc gcaaauggcc guggacaugc agacgcucac accaguggga     1140

cggcucauaa cagcgaaccc agucauaaca gaaagcacag aaaacaguaa aaugaugcuc     1200

gaacuagacc cgcccuucgg ggacuccuac auaguaaucg gcguaggcga aaagaaaaua     1260

acgcaccacu ggcaccgcuc cggcaguacc auaggaaaag cauucgaagc gacaguacga     1320

ggagccaagc gcauggcggu acugggagac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5214
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 1 opt

<400>  5214
gggagaaagc uuaccaugag ccaaaaaguc aucuaccuug uaaugauccu ccuaauagcc       60

ccagcguaca gcauccgcug caucggcgua aacaacaggg acuucguaga aggcaugucc      120

ggaggcaccu ggguagacgu cguacucgaa cacgguggau gcguaaccgu aauggcccaa      180

gacaaaccaa ccguagacau cgagcuaguc acgaccacag uaagcaacau ggcagaaguc      240

aggagcuacu gcuacgaagc aagcaucagc gacauggcaa gcgacagccg cugcccaacc      300

caaggagaag cguaccucga caaacaaagc gacacacaau acgucugcaa aagaacccuc      360

guggaccgag gcuggggcaa cggcuguggc cuauucggca aagggagccu agucacaugc      420

gccaaauucg caugcagcaa gaaaaugacc ggaaaaagca uccaacccga aaaccuagaa      480

uaccgaauaa ugcugagcgu ccacggaagc caacacucag gcaugaucgu caacgacacc      540

ggccacgaaa cagacgaaaa cagagcaaaa guagaaauca ccccaaacag ccccagagcc      600

gaagcaacac uaggcggcuu cggaagccug ggacuggacu gcgagccccg aaccggacua      660

gacuucucag accuauacua ccucacaaug aauaacaaac acuggcuagu acacaaagaa      720

ugguuccacg acauaccacu gccauggcac gccggcgcgg acaccggaac accccacugg      780

aacaacaaag aagcacucgu agaguucaaa gacgcccacg ccaaacgcca aaccgucgua      840

guacuaggca gccaagaagg agccguacac acagcccugg ccggagcacu cgaagccgaa      900

auggacgggg ccaaaggccg acucuccagc gggcaccuca aaugccgccu caaaauggac      960

aaacuacgac ucaaaggcgu aagcuacagc cucugcacag ccgcauucac auucaccaaa     1020

auacccgccg aaacccuaca cgguacagug acagucgaag uacaauacgc aggcacggac     1080

gggcccugca aaguacccgc acagauggcc guagacaugc aaacacuaac accagucggc     1140

agacucauca cagccaaccc cguuaucacc gaaagcacag aaaacagcaa aaugaugcuc     1200

gaacuagacc caccauuugg cgacagcuac auagucauug gagucggaga aaagaaaauc     1260

acacaccacu ggcacagauc cggcagcaca aucggaaaag ccuucgaagc aaccguccga     1320

ggcgcgaaac gaauggcagu acuaggagac acagcauggg acuucgguag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5215
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 1 opt

<400>  5215
gggagaaagc uuaccauguc ccaaaaagua auauaccuag uaaugauauu acucauagca       60

ccggccuaca gcauccgcug uauaggggua uccaacagag acuucgucga gggcaugagc      120

ggcggcacau gggucgacgu cguacuagag cacggcggcu gcguaacagu cauggcacaa      180

gacaaaccaa ccguggacau cgagcuaguc accaccacag ucucaaacau ggccgaaguc      240

agaagcuacu gcuacgaagc gagcaucagc gacauggcaa gcgacagcag augcccaacc      300

caaggcgagg cuuaccucga caagcagagc gacacccaau acgugugcaa acgcacgcua      360

guagacagag gcuggggaaa uggaugcgga cuuuucggaa aaggaagccu aguuacgugc      420

gcaaaauucg ccugcagcaa gaaaaugacc gggaaaucaa uccaacccga aaaccuagaa      480

uaccgaauaa ugcuaagcgu acacggcagc caacacucgg gaaugauagu aaacgacaca      540

ggacacgaga cagacgaaaa cagagccaaa guagaaauaa caccgaacuc acccagggcc      600

gaggcaacac ucggaggcuu cggcucgcua gggcucgacu gcgagcccag aaccggccua      660

gacuucuccg accuauacua ccucacaaug aauaacaaac acuggcuagu acacaaggaa      720

ugguuccacg acauaccacu gcccuggcac gcaggcgcag acacaggaac accccacugg      780

aacaacaagg aagcccuagu cgaguucaaa gacgcacacg caaagcgaca aaccguaguc      840

guccuuggaa gccaagaagg agccguacac acagcccucg caggggcccu ggaagcagaa      900

auggacggag ccaaaggcag acuauccagc ggacaccuga agugccgacu caaaauggac      960

aagcucaggc ucaagggagu cuccuacagc cuaugcgcag ccgccuucac auucacaaaa     1020

auacccgcgg aaacgcucca cggcacagua acggucgaag uccaguacgc aggcacagac     1080

ggacccugua aaguacccgc gcaaauggca gucgacaugc aaacacuaac accagucggc     1140

aggcucauaa ccgccaaccc ggucauaacc gagaguacag aaaacagcaa aaugaugcuc     1200

gaacucgauc ccccauucgg agacagcuac auaguaauag gaguaggcga aaagaaaauc     1260

acccaccacu ggcacagauc aggcucaaca auaggcaaag cauucgaagc aaccguacgc     1320

ggagcaaaac gaauggccgu ccuaggggac accgccuggg acuuugguuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5216
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 1 opt

<400>  5216
gggagaaagc uuaccauguc acaaaaagua aucuaccucg uaaugauccu acuaaucgca       60

cccgccuaca gcauacgaug caucggagua ucaaaccgcg acuucgucga agguaugucc      120

ggcggaacau ggguagacgu cgucuuagaa cacggagggu gcguaacagu cauggcccaa      180

gacaaaccaa caguagacau agagcucgua acaaccaccg uaagcaacau ggcagaaguc      240

cgcagcuacu gcuacgaggc cagcauauca gacauggccu ccgacucacg augcccaaca      300

caaggagaag cauaccuaga caaacaaagc gacacccaau acgucugcaa aagaacacug      360

gucgacagag gauggggcaa cggaugcggc cuauucggaa aaggcagccu aguaaccugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggcaaaucca uacaaccaga aaaucuagaa      480

uaccgaauaa ugcuauccgu ccacggaagc caacacuccg gcaugaucgu caacgacauc      540

ggccacgaaa ccgacgaaaa ccgagccaaa gucgaaauca cccccaacuc accccgagca      600

gaagccacac ucggcggcuu cggaucccuc ggacuagacu gcgaacccag aacaggccuc      660

gacuucagcg accucuacua ccuaacaaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccacu gcccuggcac gcaggcgccg acacaggcac gccccacugg      780

aacaacaaag aggcacucgu ggaguucaaa gacgcccacg ccaaaagaca aacaguagua      840

guacucggca gccaagaagg agccguccac acagcccucg caggagcccu cgaagccgaa      900

auggacggag ccaaaggcag acucuccagc gggcaccuga aaugccgccu caaaauggac      960

aaacuacgcc ucaaaggcgu cagcuacagc cucugcacag cagccuucac cuucaccaaa     1020

auccccgccg aaacacucca cggaaccgua acaguagaag uccaauacgc cggcacagac     1080

ggacccugca aaguacccgc acaaauggcc gucgacaugc agacccucac gcccguagga     1140

agacucauca ccgcaaaccc cgucauaacc gaaagcacag aaaacagcaa gaugaugcua     1200

gaacucgacc cacccuucgg agacagcuac aucguaauag gagugggaga caagaaaauc     1260

acacaucacu ggcaccgcag cggcucaaca auaggcaaag cauuugaagc cacaguacgc     1320

ggggccaaaa gaauggccgu ccuaggggac accgccuggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5217
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 1 opt

<400>  5217
gggagaaagc uuaccaugag ucaaaaaguc auauaccucg ucaugauccu gcuaaucgcc       60

cccgccuaca gcauacgcug uaucggagug agcaacaggg acuucguaga aggaaugagu      120

ggaggcaccu gggucgacgu aguacuagaa cacggaggau gcguaaccgu aauggcacaa      180

gacaaaccaa ccguagacau cgagcuaguc acaaccacag ucucaaacau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcaucagc gacauggcaa gcgacagccg augcccaaca      300

caaggagaag cauaccuaga caaacaaagc gacacccaau acgugugcaa acgcacccua      360

gucgaccgag gauggggcaa uggaugcggc cucuucggaa aaggaucccu agucacaugc      420

gcgaaauucg caugcagcaa gaaaaugacu ggaaaaucaa uccagccaga aaaccuagaa      480

uaccgaauca ugcucucggu acacggcagu caacacagcg guaugauagu gaacgacacc      540

ggacacgaaa ccgaugaaaa cagagccaaa gucgaaauaa ccccaaacuc ccccagagcc      600

gaagccacac uaggaggcuu cggaucgcua ggacuugacu gcgaaccaag aaccggacua      660

gacuucuccg accuauacua ucuaacaaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg auauaccacu gccauggcac gcaggagcgg acacaggaac accccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcccacg ccaaacgaca aaccgucguc      840

guacuaggca gccaggaagg agccguacac acagcccucg cgggcgcccu agaagccgaa      900

auggacggag caaaaggcag acuaucguca ggccaccuca aaugccgccu gaaaauggac      960

aaacuaaggc ucaaaggagu gucauacagc cucugcacag ccgcguucac auucacaaaa     1020

auaccagcag aaacacucca cggaacagua acaguagaag uccaauacgc aggaacagac     1080

ggacccugca aaguccccgc ccaaauggcc guagacaugc aaacccucac gccagucggc     1140

agacugauaa ccgcaaaccc aguaaucaca gaaagcacag aaaacucaaa aaugaugcuc     1200

gaacuagacc caccauucgg cgacagcuau auaguaauag gcgucggaga caagaaaauc     1260

acacaccacu gguaccgcuc aggcuccacc aucggaaaag ccuucgaagc caccguacga     1320

ggagcaaaac gcauggcagu gcuaggggac acggccuggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5218
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 1 opt

<400>  5218
gggagaaagc uuaccaugag ccaaaaagua auauaccuag uaaugauccu acuaauagcc       60

cccgcauaca gcauccgcug cauaggggua uccaacaggg acuucgucga aggcaugagc      120

ggaggcacau gggucgacgu gguccuagag cacggaggcu gcguaaccgu aauggcacaa      180

gacaaaccaa ccgucgacau cgaacugguc accaccacag uuagcaacau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcaucuca gacauggcaa gcgacucccg gugccccaca      300

caaggagaag cauaccuaga caaacagagc gacacccaau acguaugcaa acgcacacuc      360

gucgaccgag gcuggggcaa cggcugcgga cuauucggaa aaggaagccu cgucacaugc      420

gcaaaauuca caugcagcaa aaaaaugaca ggaaagagca uccaaccaga aaaccuagaa      480

uaccgcauaa ugcuaagcgu acacgguagc caacacucag gcaugauagu caacgacacc      540

ggccacgaaa ccgacgaaaa ccgagccaaa guagagguca cccccaacag cccacgagcc      600

gaagcaaccc uaggcggcuu cggcagcuua ggccucgacu gcgaaccccg caccgggcua      660

gacuucagcg accuauacua ccuaaccaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauacccuu accauggcac gccggcgcgg acacaggcac accccacugg      780

aacaacaaag aagcccucgu cgaauucaaa gacgcucacg ccaaacgcca aacaguagua      840

guacuaggca gccaggaagg cgcgguacac accgcccuag ccggagcccu agaagccgaa      900

auggacgggg ccaaaggccg acucuucagc ggacaccuga aaugccggcu gaaaauggac      960

aaacucagac uaaaaggcgu cagcuacagc cuaugcacag cagccuucac auuuacaaaa     1020

guacccgcag aaacacucca cggcaccguc acagucgaag uacaauacgc aggcacagac     1080

ggcccaugca aaguaccagc acaaauggcc guagacaugc aaacacucac accagugggc     1140

agacucauca ccgccaaccc aguaauaacc gaaagcaccg aaaacuccaa aaugaugcua     1200

gaacucgacc caccauucgg cgacuccuac auaguaauag gcgucggcga caaaaaaauc     1260

acacaccacu ggcaucgaag cggcuccaca aucggcaaag cauucgaagc aaccguaaga     1320

ggagccaaac gaauggcugu acucggcgac accgcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5219
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 1 opt

<400>  5219
gggagaaagc uuaccauguc gcaaaaaguc aucuaccuag uaaugauccu ccucauagcc       60

ccagcuuaca gcaucagaug uaucggagua uccaacagag acuucgucga aggcaugagc      120

gggggcacau gggucgacgu aguccuagag cacggcgggu gugucacugu aauggcgcaa      180

gacaaaccaa caguagacau agagcuagua acaaccaccg ucucaaacau ggccgaaguc      240

cgaagcuacu gcuaugaagc cagcauaagc gacauggcaa gcgacagcag augcccgaca      300

caaggcgaag ccuaccuaga caaacaaagc gacacccaau acguaugcaa acgcacccua      360

guagaccggg gauggggaaa cggcugcggc cuauucggga aaggaagccu aguaacaugc      420

gccaaauuca caugcagcaa gaaaaugacc gggaaaucaa uccaacccga aaaccuagaa      480

uaccgcauaa ugcugagcgu gcacggaagc caacacucag gcaugauagu aaacgacauc      540

ggccacgaaa cagacgaaaa ccgagcgaaa guagaaguga caccgaacuc acccagggca      600

gaagccacgc ucggaggcuu cgguucguua ggacuagacu gcgagcccag aacaggacuc      660

gacuuuucgg accuguacua ccuaaccaug aacaacaaac acuggcuggu acacaaagaa      720

ugguuccacg acauaccccu gccauggcac gcaggagcag acacuggcac accccacugg      780

aacaacaaag aagcacucgu ggaguucaaa gacgcccacg ccaaacgaca aaccgucguc      840

gugcucggaa gucaagaagg agcagugcac accgcccucg ccggagcacu agaagcggaa      900

auggacgggg ccaaaggccg ccucuucagc ggacaccuca aaugcaggcu gaaaauggac      960

aaacuaagac ucaagggagu aagcuacagc cucugcaccg cugcauucac cuucacgaaa     1020

guacccgcgg aaacgcuaca cggaacaguu acggucgaag uccaguacgc aggcacggac     1080

gggcccugca aagugcccgc acagauggcc guugacaugc aaacacucac cccagucggc     1140

agacucauaa cugcaaaccc ggucaucacc gaaaguacgg aaaacagcaa gaugaugcua     1200

gagcuagauc ccccauucgg ggacuccuac auagucaucg gcgugggcga caagaaaauc     1260

acgcaccacu ggcaccgauc gggcucgacc aucggcaaag ccuuugaagc aaccgugcga     1320

ggagccaaac gcauggccgu ccucggcgac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5220
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 1 opt

<400>  5220
gggagaaagc uuaccauguc ccaaaaagua auauaccucg ucaugauacu ccucauagcc       60

cccgcauaca gcauacgcug caucgggguc uccaacagag acuucgucga aggaaugagc      120

ggaggcacau ggguggacgu aguccuggaa cacggaggau gcguaaccgu aauggcccaa      180

gacaaaccaa ccgucgacau agagcuaguu acaaccaccg ucuccaacau ggccgaaguc      240

cgcuccuacu gcuacgaagc cagcauaagc gacauggcca gcgacagcag augcccgacc      300

caaggcgaag ccuaccuaga caaacaaagu gacacccaau acgucugcaa acgaacacuc      360

guagaucgcg gcuggggcaa cggcugcggu cuguucggaa aaggaagccu aguaaccugc      420

gccaaauuca caugcagcaa gaaaaugacc ggaaaaucaa uccagcccga aaaccucgaa      480

uaccgcauaa ugcuauccgu acacgguagc caacacuccg gcaugaucgu caacgacauc      540

ggacacgaga cagacgaaaa ccgcgccaaa guagaaguaa ccccaaacuc ccccagggcc      600

gaagcaacac ucggaggcuu cggaucccua ggacuagacu gcgaacccag aaccggccuc      660

gauuucagcg accuauacua ccucacgaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acauaccccu acccuggcac gccggcgcag acaccggcac accccacugg      780

aacaacaaag aagcacucgu agaguucaaa gacgcacacg caaaacgcca aaccguagua      840

guccuaggca gccaagaagg cgccguacac accgcccugg ccggagcgcu agaagcagaa      900

auggacggag ccaaaggcag acuauucagc ggccaccuaa aaugccgacu aaaaauggac      960

aaacucagac ucaaaggagu aagcuacagc cuaugcaccg ccguaugcac cgccgccaaa     1020

guccccgcag aaacacuaca cggcacaguc acaguagaag uccaauacgc aggcacggac     1080

ggccccugca aagugccagc ccaaauggcc guagacaugc aaacucugac accagucggc     1140

agacucauaa ccgccaaccc aguaauaaca gaaagcacgg aaaacucaaa aaugaugcuu     1200

gaacucgacc cgcccuucgg agacucauac auagucauag gaguaggcga caagaaaauc     1260

acacaccacu ggcaccggag cggcuccacu auaggcaaag cauucgaagc aaccguaaga     1320

ggagccaaac gaauggccgu acuaggggac acagcauggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5221
<211>  1558
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 1 opt

<400>  5221
gggagaaagc uuaccauguc gcaaaaaguc auauaccugg ucaugauccu gcuuaucgca       60

cccgcuuacu ccauccgcug caucggaguc ucgaacaggg acuucgucga aggcaugagc      120

ggaggcacau ggguagacgu aguccuagaa cacggcggcu gcgugaccgu cauggcacaa      180

gacaaaccga caguagacau agaacucguc acaaccacag ucucgaacau ggcagaaguu      240

agaaguuacu gcuacgaagc cagcauaagc gacauggcaa gcgacagcag augcccaacc      300

caaggggaag cauaccugga caaacaaagc gacacccaau acgucugcaa acgcacgcua      360

guagacaggg gauggggcaa cggaugcgga cuguucggga aaggaagccu agucacaugc      420

gcgaaguuca caugcagcaa gaaaaugaca ggaaaaucca uacaacccga aaaccuagaa      480

uaccgaauaa ugcugagcgu gcacggaagc caacacagcg gcaugauagu aaacgacgaa      540

aaccgcgcga aaguagaagu aaccccaaac agcccaaggg ccgaggcaac gcuaggagga      600

uucggaagcc uagggcucga cugcgagccc agaaccggac ucgacuucuc ggaccuauac      660

uaccuaacga ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauaccg      720

cuccccuggc acucgggagc agacaccgaa acgccacacu ggaacaacaa agaagcgcua      780

guagaguuca aagacgccca cgccaagcga cagacaguag ucguacucgg gagccaggaa      840

ggagccguac acacagcccu cgccggagcc cucgaagcag agauggacgg agccaaaggc      900

cgacuaucgu ccggccaccu aaaaugccgc cugaaaaugg acaaacucag gcuaaaaggc      960

gugagcuaca gccugugcac cgccgcauuc acauucacaa aagugcccgc agaaacacuc     1020

cauggaacag ucacagucga aguccaauac gcaggaaggg acgggcccug caaaguaccc     1080

gcgcagaugg caguagacau gcaaacgcua acccccgucg gcagacucau aacagcgaac     1140

ccggucauaa ccgaaagcac agaaaacagc aaaaugaugc uagaauuaga ucccccauuc     1200

ggggacucau acaucgucau aggcguaggc gacaagaaaa uaacacacca cuggcacaga     1260

ucaggcucca uaauaggcaa agccuucgaa gcaacagugc gaggagccaa acgcauggca     1320

guacuaggag acaccgccug ggacuucggc ucaugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau aaaaaaaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     1500

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       1558


<210>  5222
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 1 opt

<400>  5222
gggagaaagc uuaccauguc acaaaaggua auauaccuag ucaugaucgu acucauaguc       60

ccggcauaca gcauaagcug uaucggagua ucaaaccgag accugguaga gggcaugagc      120

gggggcacau ggguggacgu gguacuggaa cauggcggau gcguaaccga aauggcgcaa      180

gacaaaccga ccguggacau agagcuagua acaaugaccg ucucgaacau ggcagaaguc      240

agaagcuacu gcuacgaagc aagccugagc gacauggcaa gcgcaaguag gugcccgacc      300

caaggagaac caucacuaga caaacaaagc gacacccaga gcguaugcaa gagaacgcua      360

ggggacagag gcuggggaaa cggcugcgga aucuucggaa aaggaucccu agucacgugc      420

uccaaauuua caugcugcaa gaaaaugcca gggaaaagca uccagccaga gaaccucgaa      480

uauagaauaa ugcuacccgu gcacggcagc cagcacucag gaaugaucgu gaacgacauc      540

ggacacgaga ccgacgaaaa ccgcgcaaaa guggaaguaa ccccaaacuc acccagagca      600

gaagccacgc ucggaggauu cggaagccuc gggcucgacu gcgaaccccg aaccgggcuc      660

gacuucucag acuuauacua ccuaacaaug aacaacaagc acuggcucgu acacaaagag      720

ugguuccacg acauaccccu gccauggcac gcgggagcag acacaggcac gccgcauugg      780

aacaacaagg aggcacucgu agaguucaaa gacgcgcacg caaagcgaca aacagucgua      840

gugcucggaa gccaagaagg ggccguccac acagccuuag ccggggcccu cgaagcagaa      900

auggacgggg caaagggccg gcucuuuagc ggccaccuaa aaugccgccu aaaaauggac      960

aagcuaagac uaaagggcgu aagcuacagc cugugcacag ccgcauucac auucacaaaa     1020

guacccgcag aaacgcucca cggcacaguc acagucgagg uccagagcgc gggaacggac     1080

ggacccugca aaguaccagc gcaaauggcc guagacaugc aaacacucac ccccguggga     1140

aggcuaauaa cagcaaaccc gguaauaacc gaaagcacag aaaacagcaa gaugaugcuc     1200

gaguuagacc caccauucgg ggacucauac auaguuauag gcguaggcga caagaaaauc     1260

acgcaccauu ggcacagauc cggaaguacc aucggcaaag ccuucgaagc aaccgugcgc     1320

ggggccaaga gaauggcagu gcucggagau accgcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5223
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 1 opt

<400>  5223
gggagaaagc uuaccaugcg acaaaaaguc auuuaccuag uaaugauacu ccuaauagcc       60

ccagccuaca gcauuagaug caucggggua ucaaacaggg acuucguaga aggaaugagc      120

ggcggaaccu ggguagacgu cguccucgaa cacggcggcu gcguaaccgu aauggcacaa      180

gacaaaccga ccguagacau agaacucguc accaccacag ucucaaacau ggcagaaguc      240

cggagcuacu gcuacgaggc uucuauaagc gacauggcaa gcgacagcag augcccaacc      300

caaggcgaag cauaccuaga caaacaaagc gacacccagu acguaugcaa aagaacccuc      360

guagaccgag gauggggcaa cggcugcggc cuguucggaa aaggaagccu gguaacaugc      420

gcaaaauuug ccugcagcaa aaagaugaca gggaaaucca uccaacccga aaaccuagaa      480

uacagaauaa ugcucagcgu acauggaagc caacauagcg gcaugaucgu caacgacaca      540

ggacacgaga cagacgaaaa ccgcgcaaaa guagaaauca ccccaaacuc acccagggcc      600

gaggcaacac ucggcggauu cggcagccuc ggccucgacu gcgaacccag aaccggccuc      660

gacuucucag accuguacua ucuaaccaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acaucccacu gcccuggcac gccggcgccg acacaggaac accacacugg      780

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg caaaacgaca aacagucguc      840

guccucggca gccaagaagg agcaguccac acggcccugg caggagcccu cgaagcagaa      900

auggacggag ccaaaggcag gcucucauca ggacaccuaa aaugccgccu uaaaauggac      960

aaacuccgac ugaaaggagu auccuacagc cucugcacag ccgcauucac cuucacaaaa     1020

auaccagcgg aaacacugca cggcacaguc accgucgaag uccaguacgc aggcaccgac     1080

ggccccugca aaguaccagc acaaauggcg guggacaugc aaacccuaac cccaguaggc     1140

cgacuaauaa ccgcgaaccc agucauaacc gagagcaccg aaaacaguaa aaugaugcuc     1200

gagcucgacc caccauucgg agacagcuac auaguaauag gcgucgguga aaagaaaauc     1260

acacaccacu ggcaccgcuc gggcucaacc aucggcaaag ccuucgaagc aacuguacgc     1320

ggagccaaac gcauggcagu acuaggagac acagcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5224
<211>  1576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 1 opt

<400>  5224
gggagaaagc uuaccaugag ccaaagaguc aucuaccugg uaaugauccu acuaauagcc       60

ccagccuaca gcauccgaug caucgggguc ucgaacagag acuucgucga aggcaugagc      120

ggaggcacau ggguagacgu agugcuggaa cacggcggau gcguaaccgu aauggcacaa      180

gacaaaccaa ccguagacau agagcuaguc acaacaacag ucucaaacau ggccgaaguc      240

aggaguuacu gcuacgaagc aagcauaagc gacauggcca gcgacagccg augcccaacc      300

caaggcgaag cauaccuaga caaacaaagc gacacccaau acgugugcaa acgcacgcua      360

gucgacagag gauggggcaa cgggugcggc cucuucggga aaggaagccu agucacgugc      420

gcaaaauuca caugcagcaa gaagaugacc ggaaaaucaa uccaacccga aaaccuagaa      480

uaccgcauaa ugcugagcgu ccacggaagc caacacucag gcaugaucgu caacgacauc      540

ggacacgaga ccgacgaaaa ccgagcgaaa guggaaguaa ccccaaacuc accaagggcc      600

gaggccaccc ucggaggcuu cggaucgcuc gggcuagacu gcgaacccag aaccggacuc      660

gacuucucag accucuacua ccucacgaug aacaacaaac auuggcucgu gcacaaagaa      720

ugguuccacg auaucccacu gcccuggcac gcaggcgcgg acacaggaac accccacugg      780

aacaauaagg aagcacuugu agaguucaaa gacgcccacg ccaaacgcca gaccguaguc      840

guacuaggaa gccaagaagg agccgugcac accgcccugg caggagcacu ggaagcagaa      900

auggacggag ccaaaggccg acucuucagc ggacacuuga aaugccgcau aaaaauggac      960

aaacucaggc ucaaaggcgu gagcuacagc cuaugcacug ccgcauucac auucuccaag     1020

guaccagcag aaacacucca cggcaccguc acgguagaag uccaauacgc aggccgagac     1080

gggccaugca aagugcccgc acaaauggcc guagacaugc aaacgcucac acccguaggc     1140

aggcucauaa cagcgaaccc aaucauaaca gaaaguacag aaaacagcag aaugaugcua     1200

gaacucgacc caccguucgg ggacuccuac auagucaucg gcgugggcga caagaaaaua     1260

acacaccacu ggcauagauc gggcucaacc auaggcaaag ccuucgaagc gacagugcga     1320

ggagcaaaaa gaauggccgu acuaggagac acagcauggg auuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     1560

cagagccacc agaauu                                                     1576


<210>  5225
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4797 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2
       wild type

<400>  5225
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac ggggcgcaga uaccaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5226
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 wild 
       type

<400>  5226
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacgaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacacgug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aaucuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacgcaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5227
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 wild type

<400>  5227
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg     1140

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga aguuuaggac     1200

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca     1260

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uuuaaggaug     1380

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg     1440

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc     1500

auuugaaaug ccgccuaaaa auggacaagc ucagauugaa gggcguguca uauuccuugu     1560

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1620

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1680

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1740

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1800

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1860

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1920

ccugggacuu cggaucaguc gggggugugu ucaacucacu ggguaagggc auucaccaga     1980

uuuuuggagc agccuucaaa ucacuguuug gaggaauguc cugguucuca cagauccuca     2040

uaggcacgcu gcuagugugg uuagguuuga acacaaagaa uggaucuauc ucccucacau     2100

gcuuggcccu ggggggagug augaucuucc ucuccacggc uguuucugcu gacguggggu     2160

gcucagugga cuucucaaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5228
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 2 wild 
       type

<400>  5228
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uaccaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguuggucc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5229
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 wild
       type

<400>  5229
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5230
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 2 wild 
       type

<400>  5230
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac acgugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac uguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaaucu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaacg     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5231
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 2 wild type

<400>  5231
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau uggauaugaa acugacgaag     1080

auagagcgaa agucgagguu acgccuaauu caccaagagc ggaagcaacc uugggaggcu     1140

uuggaaguuu aggacuugac ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu     1200

accugaccau gaacaauaag cauugguugg ugcacaaaga gugguuucau gacaucccau     1260

ugccuuggca ugcuggggca gacaccggaa cuccacacug gaacaacaaa gaggcauugg     1320

uagaauuuaa ggaugcccac gccaagaggc aaaccgucgu cguucugggg agccaggaag     1380

gagccguuca cacggcucuc gcuggagcuc uagaggcuga gauggauggu gcaaagggaa     1440

ggcuguucuc uggccauuug aaaugccgcc uaaaaaugga caagcucaga uugaagggcg     1500

ugucauauuc cuugugcacu gcggcauuca cauucaccaa ggucccagcu gaaacacugc     1560

auggaacagu cacaguggag gugcaguaug cagggacaga uggacccugc aagaucccag     1620

uccagauggc gguggacaug cagacccuga ccccaguugg aaggcugaua accgccaacc     1680

ccgugauuac ugaaagcacu gagaacucaa agaugauguu ggagcuugac ccaccauuug     1740

gggauucuua cauugucaua ggaguugggg acaagaaaau cacccaccac uggcauagga     1800

gugguagcac caucggaaag gcauuugagg ccacugugag aggcgccaag agaauggcag     1860

uccuggggga uacagccugg gacuucggau cagucggggg uguguucaac ucacugggua     1920

agggcauuca ccagauuuuu ggagcagccu ucaaaucacu guuuggagga auguccuggu     1980

ucucacagau ccucauaggc acgcugcuag ugugguuagg uuugaacaca aagaauggau     2040

cuaucucccu cacaugcuug gcccuggggg gagugaugau cuuccucucc acggcuguuu     2100

cugcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5232
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 2 wild 
       type

<400>  5232
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5233
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 wild
       type

<400>  5233
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5234
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 wild 
       type

<400>  5234
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5235
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 wild type

<400>  5235
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      240

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      420

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      480

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc      540

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg      600

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga aguuuaggac      660

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca      720

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug      780

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uuuaaggaug      840

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg      900

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc      960

auuugaaaug ccgccuaaaa auggacaagc ucagauugaa gggcguguca uauuccuugu     1020

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1080

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1140

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1200

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1260

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1320

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1380

ccugggacuu cggaucauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  5236
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 2 wild 
       type

<400>  5236
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguuggucc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5237
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       2 wild type

<400>  5237
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuccg gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5238
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 2
       wild type

<400>  5238
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac acgugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac uguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuccg gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaaucu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaacg     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5239
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 2 
       wild type

<400>  5239
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuccg gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau uggauaugaa acugacgaag     1080

auagagcgaa agucgagguu acgccuaauu caccaagagc ggaagcaacc uugggaggcu     1140

uuggaaguuu aggacuugac ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu     1200

accugaccau gaacaauaag cauugguugg ugcacaaaga gugguuucau gacaucccau     1260

ugccuuggca ugcuggggca gacaccggaa cuccacacug gaacaacaaa gaggcauugg     1320

uagaauuuaa ggaugcccac gccaagaggc aaaccgucgu cguucugggg agccaggaag     1380

gagccguuca cacggcucuc gcuggagcuc uagaggcuga gauggauggu gcaaagggaa     1440

ggcuguucuc uggccauuug aaaugccgcc uaaaaaugga caagcucaga uugaagggcg     1500

ugucauauuc cuugugcacu gcggcauuca cauucaccaa ggucccagcu gaaacacugc     1560

auggaacagu cacaguggag gugcaguaug cagggacaga uggacccugc aagaucccag     1620

uccagauggc gguggacaug cagacccuga ccccaguugg aaggcugaua accgccaacc     1680

ccgugauuac ugaaagcacu gagaacucaa agaugauguu ggagcuugac ccaccauuug     1740

gggauucuua cauugucaua ggaguugggg acaagaaaau cacccaccac uggcauagga     1800

gugguagcac caucggaaag gcauuugagg ccacugugag aggcgccaag agaauggcag     1860

uccuggggga uacagccugg gacuucggau cagucggggg uguguucaac ucacugggua     1920

agggcauuca ccagauuuuu ggagcagccu ucaaaucacu guuuggagga auguccuggu     1980

ucucacagau ccucauaggc acgcugcuag ugugguuagg uuugaacaca aagaauggau     2040

cuaucucccu cacaugcuug gcccuggggg gagugaugau cuuccucucc acggcuguuu     2100

cugcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5240
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 2 
       wild type

<400>  5240
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuccg gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5241
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       2 wild type

<400>  5241
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguucaggac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5242
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 2
       wild type

<400>  5242
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac acgugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac uguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguucaggac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaaucu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaacg     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5243
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 2 
       wild type

<400>  5243
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguucaggac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5244
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 wild 
       type

<400>  5244
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc acgcugggca aggccuuuuc aacaacuuug aagggagcuc     1860

aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga ggggucuuca     1920

acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca cucuuugggg     1980

gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg     2040

cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc guguucuuag     2100

cgaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5245
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 wild 
       type

<400>  5245
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac acgugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac uguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc acgcugggca aggccuuuuc aacaacuuug aagggagcuc     1860

aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga ggggucuuca     1920

acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca cucuuugggg     1980

gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg     2040

cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc guguucuuag     2100

cgaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5246
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 2 wild type

<400>  5246
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau uggauaugaa acugacgaag     1080

auagagcgaa agucgagguu acgccuaauu caccaagagc ggaagcaacc uugggaggcu     1140

uuggaaguuu aggacuugac ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu     1200

accugaccau gaacaauaag cauugguugg ugcacaaaga gugguuucau gacaucccau     1260

ugccuuggca ugcuggggca gacaccggaa cuccacacug gaacaacaaa gaggcauugg     1320

uagaauuuaa ggaugcccac gccaagaggc aaaccgucgu cguucugggg agccaggaag     1380

gagccguuca cacggcucuc gcuggagcuc uagaggcuga gauggauggu gcaaagggaa     1440

ggcuguucuc uggccauuug aaaugccgcc uaaaaaugga caagcucaga uugaagggcg     1500

ugucauauuc cuugugcacu gcggcauuca cauucaccaa ggucccagcu gaaacacugc     1560

auggaacagu cacaguggag gugcaguaug cagggacaga uggacccugc aagaucccag     1620

uccagauggc gguggacaug cagacccuga ccccaguugg aaggcugaua accgccaacc     1680

ccgugauuac ugaaagcacu gagaacucaa agaugauguu ggagcuugac ccaccauuug     1740

gggauucuua cauugucaua ggaguugggg acaagaaaau cacccaccac uggcauagga     1800

guggcagcac gcugggcaag gccuuuucaa caacuuugaa gggagcucaa agacuggcag     1860

cguugggcga cacagccugg gacuuuggcu cuauuggagg ggucuucaac uccauaggaa     1920

gagccguuca ccaaguguuu gguggugccu ucagaacacu cuuuggggga augucuugga     1980

ucacacaagg gcuaaugggu gcccuacugc ucuggauggg cgucaacgca cgagaccgau     2040

caauugcuuu ggccuucuua gccacaggag gugugcucgu guucuuagcg accaaugugc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5247
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 wild 
       type

<400>  5247
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc acgcugggca aggccuuuuc aacaacuuug aagggagcuc     1860

aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga ggggucuuca     1920

acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca cucuuugggg     1980

gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg     2040

cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc guguucuuag     2100

cgaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5248
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 wild 
       type

<400>  5248
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac ggggcgcaga uaccaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5249
<211>  2521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5249
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

ucaaugcuag gaaggagaag aagagacggg gcgcagauac cagugucgga auuguuggcc      120

uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca uacuauaugu      180

acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug gggaugaaua      240

aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug agcuaugaau      300

gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc aacacgacgu      360

caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg agaucuagaa      420

gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg caaaccuggu      480

uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua uucaggaacc      540

cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa      600

aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc aggugcauag      660

gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu gauauugucu      720

uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc gacauagagc      780

ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau gaggcaucaa      840

uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac cuugacaagc      900

aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg ggaaauggau      960

guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc uccaagaaaa     1020

ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug ucaguucaug     1080

gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau gagaauagag     1140

cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg gguuuuggaa     1200

gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug uauuacuuga     1260

cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu ccauuaccuu     1320

ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca cugguagagu     1380

ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa gaaggagcag     1440

uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag ggaaggcugu     1500

ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag ggcgugucau     1560

acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca cugcacggga     1620

cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu ccagcucaga     1680

uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu aaccccguaa     1740

ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca uuuggggacu     1800

cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac aggaguggca     1860

gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug gcagucuugg     1920

gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug ggcaagggca     1980

uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc ugguucucac     2040

aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau ggaucuauuu     2100

cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc gucucugcug     2160

auguggggug cucgguggac uucucaaagu gaggacuagu gcaucacauu uaaaagcauc     2220

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2280

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2340

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2460

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2520

u                                                                     2521


<210>  5250
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5250
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu guuggccucc      120

ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac uauauguacu      180

uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg augaauaagu      240

guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc uaugaaugcc      300

cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac acgacgucaa      360

cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga ucuagaagag      420

cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa accugguugg      480

aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc aggaacccug      540

gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag      600

ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg ugcauaggag      660

ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau auugucuugg      720

aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac auagagcugg      780

uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag gcaucaauau      840

cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu gacaagcaau      900

cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga aauggaugug      960

gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga     1020

ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca guucauggcu     1080

cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag aauagagcga     1140

agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu uuuggaagcc     1200

uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau uacuugacua     1260

ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca uuaccuuggc     1320

acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug guagaguuca     1380

aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa ggagcaguuc     1440

acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga aggcuguccu     1500

cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc gugucauacu     1560

ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug cacgggacag     1620

ucacagugga gguacaguac gcagggacag auggaccuug caagguucca gcucagaugg     1680

cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac cccguaauca     1740

cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu ggggacucuu     1800

acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg aguggcagca     1860

ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca gucuugggag     1920

acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc aagggcaucc     1980

aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg uucucacaaa     2040

uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga ucuauuuccc     2100

uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc ucugcugaug     2160

uggggugcuc gguggacuuc ucaaagugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  5251
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5251
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaug       60

cuaggaagga gaagaagaga cggggcgcag auaccagugu cggaauuguu ggccuccugc      120

ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau auguacuugg      180

acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug aauaaguguu      240

auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau gaaugcccua      300

ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg acgucaacuu      360

ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu agaagagcug      420

ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau      480

caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg aacccuggcu      540

ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc caaaaaguca      600

uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc auaggaguca      660

gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu gucuuggaac      720

auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua gagcugguua      780

caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca ucaauaucag      840

acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac aagcaaucag      900

acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau ggauguggac      960

uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag aaaaugaccg     1020

ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu cauggcuccc     1080

agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau agagcgaagg     1140

uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu ggaagccuag     1200

gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac uugacuauga     1260

auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua ccuuggcacg     1320

cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua gaguucaagg     1380

acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga gcaguucaca     1440

cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg cuguccucug     1500

gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug ucauacuccu     1560

uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac gggacaguca     1620

caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu cagauggcgg     1680

uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc guaaucacug     1740

aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg gacucuuaca     1800

uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu ggcagcacca     1860

uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc uugggagaca     1920

cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag ggcauccauc     1980

aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc ucacaaauuc     2040

ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu auuucccuua     2100

ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu gcugaugugg     2160

ggugcucggu ggacuucuca aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5252
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5252
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcua       60

ggaaggagaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc cuccugcuga      120

ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug uacuuggaca      180

gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau aaguguuaua      240

uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa ugcccuaugc      300

uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg ucaacuuggg      360

uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga agagcuguga      420

cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa      480

gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac ccuggcuucg      540

cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau      600

acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua ggagucagca      660

auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc uuggaacaug      720

gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag cugguuacaa      780

caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca auaucagaca      840

uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag caaucagaca      900

cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga uguggacuuu      960

uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa augaccggga     1020

agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau ggcucccagc     1080

acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga gcgaagguug     1140

agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga agccuaggac     1200

uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug acuaugaaua     1260

acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu uggcacgcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag uucaaggacg     1380

cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca guucacacgg     1440

cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug uccucuggcc     1500

acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca uacuccuugu     1560

guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg acagucacag     1620

uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag auggcggugg     1680

acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua aucacugaaa     1740

gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac ucuuacauug     1800

ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc agcaccauug     1860

gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug ggagacacag     1920

ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa     1980

uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca caaauucuca     2040

uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu ucccuuaugu     2100

gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu gauguggggu     2160

gcucggugga cuucucaaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5253
<211>  2509
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5253
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

aggagaagaa gagacggggc gcagauacca gugucggaau uguuggccuc cugcugacca      120

cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac uuggacagaa      180

acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag uguuauauac      240

agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc ccuaugcugg      300

augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca acuuggguug      360

uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga gcugugacgc      420

uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug gaaucaagag      480

aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu ggcuucgcgu      540

uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa gucauauacu      600

uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga gucagcaaua      660

gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug gaacauggag      720

guugugucac cguaauggca caggacaaac cgacugucga cauagagcug guuacaacaa      780

cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua ucagacaugg      840

cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa ucagacacuc      900

aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu ggacuuuuug      960

gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga     1020

gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc ucccagcaca     1080

gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg aagguugaga     1140

uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc cuaggacuug     1200

auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu augaauaaca     1260

agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg cacgcugggg     1320

cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc aaggacgcac     1380

augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu cacacggccc     1440

uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc ucuggccacu     1500

ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac uccuugugua     1560

ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca gucacagugg     1620

agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug gcgguggaca     1680

ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc acugaaagca     1740

cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu uacauuguca     1800

uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc accauuggaa     1860

aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga gacacagccu     1920

gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc caucaaauuu     1980

uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa auucucauug     2040

gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc cuuaugugcu     2100

uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau guggggugcu     2160

cgguggacuu cucaaaguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2220

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2280

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2340

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2460

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2509


<210>  5254
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5254
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagag acggggcgca gauaccagug ucggaauugu uggccuccug cugaccacag      120

cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug gacagaaacg      180

augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu uauauacaga      240

ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu augcuggaug      300

agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu uggguugugu      360

acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu gugacgcucc      420

ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau      480

acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc uucgcguuag      540

cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg      600

ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc agcaauaggg      660

acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa cauggagguu      720

gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu acaacaacag      780

ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca gacauggcuu      840

cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca gacacucaau      900

augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga cuuuuuggca      960

aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc gggaagagca     1020

uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc cagcacagug     1080

ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag guugagauaa     1140

cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua ggacuugauu     1200

gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug aauaacaagc     1260

acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac gcuggggcag     1320

acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag gacgcacaug     1380

ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac acggcccuug     1440

cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu ggccacuuga     1500

aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc uuguguaccg     1560

cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc acaguggagg     1620

uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg guggacaugc     1680

aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu gaaagcacug     1740

agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac auugucauag     1800

gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc auuggaaaag     1860

cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac acagccuggg     1920

acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau caaauuuuug     1980

gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu cucauuggaa     2040

cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu augugcuugg     2100

ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugaugug gggugcucgg     2160

uggacuucuc aaagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5255
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5255
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagagacg gggcgcagau accagugucg gaauuguugg ccuccugcug accacagcua      120

uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac agaaacgaug      180

cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau auacagauca      240

uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug cuggaugagg      300

ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg guuguguacg      360

gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug acgcuccccu      420

cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca agagaauaca      480

caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc gcguuagcag      540

cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua uacuugguca      600

ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc aauagggacu      660

uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau ggagguugug      720

ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca acaacaguca      780

gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac auggcuucgg      840

acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac acucaauaug      900

ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu uuuggcaaag      960

ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg aagagcaucc     1020

agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag cacaguggga     1080

ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu gagauaacgc     1140

ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga cuugauugug     1200

aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau aacaagcacu     1260

gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu ggggcagaca     1320

ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac gcacaugcca     1380

aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg gcccuugcug     1440

gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc cacuugaaau     1500

gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug uguaccgcag     1560

cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca guggagguac     1620

aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug gacaugcaaa     1680

cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa agcacugaga     1740

acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu gucauaggag     1800

ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu ggaaaagcau     1860

uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca gccugggacu     1920

uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa auuuuuggag     1980

cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc auuggaacgu     2040

ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug ugcuuggccu     2100

uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg ugcucggugg     2160

acuucucaaa gugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  5256
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5256
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agagacgggg cgcagauacc agugucggaa uuguuggccu ccugcugacc acagcuaugg      120

cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga gguuguguca      720

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu     1260

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1860

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1920

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu uuuggagcag     1980

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu ggaacguugc     2040

ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     2100

ggggaguguu gaucuucuua uccacagccg ucucugcuga uguggggugc ucgguggacu     2160

ucucaaagug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  5257
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5257
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gacggggcgc agauaccagu gucggaauug uuggccuccu gcugaccaca gcuauggcag      120

cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu ugugucaccg      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggaguc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1260

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     1860

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     1920

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu ggagcagcuu     1980

ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga acguugcuga     2040

ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2100

gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcucg guggacuucu     2160

caaagugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5258
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5258
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgcaga uaccaguguc ggaauuguug gccuccugcu gaccacagcu auggcagcgg      120

aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau gcuggggagg      180

ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc auggaucuug      240

gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag gggguggaac      300

cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac ggaaccugcc      360

aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc ucccauucca      420

cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac acaaagcacu      480

ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca gcagcugcca      540

ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc augauacugc      600

ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac uuuguggaag      660

guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu gucaccguaa      720

uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc agcaacaugg      780

cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg gacagccgcu      840

gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau gucugcaaaa      900

gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa gggagucugg      960

ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc cagccagaga     1020

aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg augaucguua     1080

augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg cccaauucac     1140

caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu gaaccgagga     1200

caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac ugguugguuc     1260

acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac accggaacuc     1320

cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc aaaaggcaaa     1380

cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu ggagcucugg     1440

aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa ugucgccuga     1500

aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca gcguucacau     1560

ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua caguacgcag     1620

ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa acucugaccc     1680

caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag aacucuaaga     1740

ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga gucggggaga     1800

agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca uuugaagcca     1860

cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac uuuggaucag     1920

uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga gcagcuuuca     1980

aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg uugcugaugu     2040

gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc uuagggggag     2100

uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug gacuucucaa     2160

agugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5259
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5259
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug gcagcggagg      120

ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu ggggaggcca      180

uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug gaucuuggac      240

acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg guggaaccag      300

augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga accugccauc      360

acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc cauuccacua      420

ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca aagcacuuga      480

uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca gcugccaucg      540

cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug auacugcuga      600

uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu guggaaggua      660

ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc accguaaugg      720

cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc aacauggcgg      780

agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac agccgcugcc      840

caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc ugcaaaagaa      900

cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg agucugguga      960

caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag ccagagaauc     1020

uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug aucguuaaug     1080

acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc aauucaccaa     1140

gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa ccgaggacag     1200

gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg uugguucaca     1260

aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac     1320

acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa aggcaaacug     1380

ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga gcucuggagg     1440

cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa     1500

uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg uucacauuca     1560

ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag uacgcaggga     1620

cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu cugaccccag     1680

uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac ucuaagauga     1740

ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc ggggagaaga     1800

agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu gaagccacug     1860

ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu ggaucaguug     1920

gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca gcuuucaaau     1980

cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug cugauguggu     2040

ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua gggggagugu     2100

ugaucuucuu auccacagcc gucucugcug auguggggug cucgguggac uucucaaagu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5260
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5260
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

cagauaccag ugucggaauu guuggccucc ugcugaccac agcuauggca gcggagguca      120

cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg gaggccauau      180

cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau cuuggacaca      240

ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug gaaccagaug      300

acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc ugccaucaca      360

aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau uccacuagga      420

agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag cacuugauua      480

gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu gccaucgcuu      540

ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua cugcugauug      600

ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug gaagguaugu      660

caggugggac uuggguugau auugucuugg aacauggagg uugugucacc guaauggcac      720

aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac auggcggagg      780

uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc cgcugcccaa      840

cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc aaaagaacgu      900

uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu cuggugacau      960

gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca gagaaucugg     1020

aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc guuaaugaca     1080

caggacauga aacugaugag aauagagcga agguugagau aacgcccaau ucaccaagag     1140

ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg aggacaggcc     1200

uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug guucacaagg     1260

agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga acuccacacu     1320

ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg caaacugucg     1380

ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu cuggaggcug     1440

agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg     1500

auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc acauucacca     1560

agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac gcagggacag     1620

auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug accccaguug     1680

ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu aagaugaugc     1740

uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg gagaagaaga     1800

ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa gccacuguga     1860

gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga ucaguuggag     1920

gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu uucaaaucau     1980

uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug augugguugg     2040

gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga     2100

ucuucuuauc cacagccguc ucugcugaug uggggugcuc gguggacuuc ucaaagugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5261
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5261
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcag       60

auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg gaggucacua      120

gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag gccauaucuu      180

uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu ggacacaugu      240

gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa ccagaugacg      300

ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc caucacaaaa      360

aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc acuaggaagc      420

ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac uugauuagag      480

ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc      540

uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug cugauugccc      600

cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa gguaugucag      660

gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua auggcacagg      720

acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug gcggagguaa      780

gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc ugcccaacac      840

aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa agaacguuag      900

uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug gugacaugcg      960

cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag aaucuggagu     1020

accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu aaugacacag     1080

gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca ccaagagccg     1140

aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg acaggccuug     1200

acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu cacaaggagu     1260

gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu ccacacugga     1320

acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa acugucgugg     1380

uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug gaggcugaga     1440

uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug aaaauggaua     1500

aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca uucaccaaga     1560

ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca gggacagaug     1620

gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc ccaguuggga     1680

gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag augaugcugg     1740

aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag aagaagauca     1800

cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc acugugagag     1860

gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca guuggaggcg     1920

cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu     1980

uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug ugguuggguc     2040

ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga guguugaucu     2100

ucuuauccac agccgucucu gcugaugugg ggugcucggu ggacuucuca aagugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5262
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5262
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaua       60

ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag gucacuagac      120

gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc      180

caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga cacaugugug      240

augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca gaugacgucg      300

auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau cacaaaaaag      360

gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu aggaagcugc      420

aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug auuagagucg      480

aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu      540

ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug auugccccgg      600

cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu augucaggug      660

ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug gcacaggaca      720

aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg gagguaagau      780

ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc ccaacacaag      840

gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga acguuagugg      900

acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug acaugcgcua      960

aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau cuggaguacc     1020

ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau gacacaggac     1080

augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca agagccgaag     1140

ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca ggccuugacu     1200

uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac aaggaguggu     1260

uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca cacuggaaca     1320

acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu gucgugguuc     1380

uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag gcugagaugg     1440

auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa auggauaaac     1500

uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc accaagaucc     1560

cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg acagauggac     1620

cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca guugggaggu     1680

ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug augcuggaac     1740

uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag aagaucaccc     1800

accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu gugagaggug     1860

ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu ggaggcgcuc     1920

ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug     1980

gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg uugggucuga     2040

acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug uugaucuucu     2100

uauccacagc cgucucugcu gauguggggu gcucggugga cuucucaaag ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5263
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5263
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcugau guggggugcu cgguggacuu cucaaaguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5264
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5264
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggccguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcugau guggggugcu cgguggacuu cucaaaguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5265
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5265
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug gcagcggccg      120

ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu ggggaggcca      180

uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug gaucuuggac      240

acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg guggaaccag      300

augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga accugccauc      360

acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc cauuccacua      420

ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca aagcacuuga      480

uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca gcugccaucg      540

cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug auacugcuga      600

uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu guggaaggua      660

ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc accguaaugg      720

cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc aacauggcgg      780

agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac agccgcugcc      840

caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc ugcaaaagaa      900

cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg agucugguga      960

caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag ccagagaauc     1020

uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug aucguuaaug     1080

acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc aauucaccaa     1140

gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa ccgaggacag     1200

gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg uugguucaca     1260

aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac     1320

acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa aggcaaacug     1380

ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga gcucuggagg     1440

cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa     1500

uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg uucacauuca     1560

ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag uacgcaggga     1620

cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu cugaccccag     1680

uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac ucuaagauga     1740

ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc ggggagaaga     1800

agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu gaagccacug     1860

ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu ggaucaguug     1920

gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca gcuuucaaau     1980

cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug cugauguggu     2040

ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua gggggagugu     2100

ugaucuucuu auccacagcc gucucugcug auguggggug cucgguggac uucucaaagu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5266
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5266
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuguccuc aaggaaacgc cguucccaug auguucugac ugugcaauuc cuaauuuugg      120

gaaugcuguu gaugacgggu ggagugaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcuccgu cgacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5267
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5267
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuguccuc aaggaaacgc cguucccaug auguucugac ugugcaauuc cuaauuuugg      120

gaaugcuguu gaugacgggu gccgugaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcuccgu cgacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5268
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5268
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac ggggcgcaga uaccaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5269
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5269
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

ucaaugcuag gaaggagaag aagagacggg gcgcagauac cagugucgga auuguuggcc      120

uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca uacuauaugu      180

acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug gggaugaaua      240

aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug agcuaugaau      300

gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc aacacgacgu      360

caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg agaucuagaa      420

gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg caaaccuggu      480

uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua uucaggaacc      540

cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa      600

aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc aggugcauag      660

gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu gauauugucu      720

uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc gacauagagc      780

ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau gaggcaucaa      840

uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac cuugacaagc      900

aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg ggaaauggau      960

guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc uccaagaaaa     1020

ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug ucaguucaug     1080

gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau gagaauagag     1140

cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg gguuuuggaa     1200

gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug uauuacuuga     1260

cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu ccauuaccuu     1320

ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca cugguagagu     1380

ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa gaaggagcag     1440

uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag ggaaggcugu     1500

ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag ggcgugucau     1560

acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca cugcacggga     1620

cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu ccagcucaga     1680

uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu aaccccguaa     1740

ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca uuuggggacu     1800

cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac aggaguggca     1860

gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug gcagucuugg     1920

gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug ggcaagggca     1980

uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc ugguucucac     2040

aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau ggaucuauuu     2100

cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc gucucugcug     2160

auguggggug cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  5270
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5270
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu guuggccucc      120

ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac uauauguacu      180

uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg augaauaagu      240

guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc uaugaaugcc      300

cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac acgacgucaa      360

cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga ucuagaagag      420

cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa accugguugg      480

aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc aggaacccug      540

gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag      600

ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg ugcauaggag      660

ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau auugucuugg      720

aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac auagagcugg      780

uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag gcaucaauau      840

cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu gacaagcaau      900

cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga aauggaugug      960

gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga     1020

ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca guucauggcu     1080

cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag aauagagcga     1140

agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu uuuggaagcc     1200

uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau uacuugacua     1260

ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca uuaccuuggc     1320

acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug guagaguuca     1380

aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa ggagcaguuc     1440

acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga aggcuguccu     1500

cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc gugucauacu     1560

ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug cacgggacag     1620

ucacagugga gguacaguac gcagggacag auggaccuug caagguucca gcucagaugg     1680

cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac cccguaauca     1740

cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu ggggacucuu     1800

acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg aguggcagca     1860

ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca gucuugggag     1920

acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc aagggcaucc     1980

aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg uucucacaaa     2040

uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga ucuauuuccc     2100

uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc ucugcugaug     2160

uggggugcug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  5271
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5271
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaug       60

cuaggaagga gaagaagaga cggggcgcag auaccagugu cggaauuguu ggccuccugc      120

ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau auguacuugg      180

acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug aauaaguguu      240

auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau gaaugcccua      300

ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg acgucaacuu      360

ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu agaagagcug      420

ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau      480

caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg aacccuggcu      540

ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc caaaaaguca      600

uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc auaggaguca      660

gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu gucuuggaac      720

auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua gagcugguua      780

caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca ucaauaucag      840

acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac aagcaaucag      900

acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau ggauguggac      960

uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag aaaaugaccg     1020

ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu cauggcuccc     1080

agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau agagcgaagg     1140

uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu ggaagccuag     1200

gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac uugacuauga     1260

auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua ccuuggcacg     1320

cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua gaguucaagg     1380

acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga gcaguucaca     1440

cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg cuguccucug     1500

gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug ucauacuccu     1560

uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac gggacaguca     1620

caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu cagauggcgg     1680

uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc guaaucacug     1740

aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg gacucuuaca     1800

uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu ggcagcacca     1860

uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc uugggagaca     1920

cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag ggcauccauc     1980

aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc ucacaaauuc     2040

ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu auuucccuua     2100

ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu gcugaugugg     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5272
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5272
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcua       60

ggaaggagaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc cuccugcuga      120

ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug uacuuggaca      180

gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau aaguguuaua      240

uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa ugcccuaugc      300

uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg ucaacuuggg      360

uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga agagcuguga      420

cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa      480

gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac ccuggcuucg      540

cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau      600

acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua ggagucagca      660

auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc uuggaacaug      720

gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag cugguuacaa      780

caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca auaucagaca      840

uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag caaucagaca      900

cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga uguggacuuu      960

uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa augaccggga     1020

agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau ggcucccagc     1080

acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga gcgaagguug     1140

agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga agccuaggac     1200

uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug acuaugaaua     1260

acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu uggcacgcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag uucaaggacg     1380

cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca guucacacgg     1440

cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug uccucuggcc     1500

acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca uacuccuugu     1560

guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg acagucacag     1620

uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag auggcggugg     1680

acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua aucacugaaa     1740

gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac ucuuacauug     1800

ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc agcaccauug     1860

gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug ggagacacag     1920

ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa     1980

uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca caaauucuca     2040

uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu ucccuuaugu     2100

gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu gauguggggu     2160

gcugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5273
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5273
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

aggagaagaa gagacggggc gcagauacca gugucggaau uguuggccuc cugcugacca      120

cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac uuggacagaa      180

acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag uguuauauac      240

agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc ccuaugcugg      300

augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca acuuggguug      360

uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga gcugugacgc      420

uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug gaaucaagag      480

aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu ggcuucgcgu      540

uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa gucauauacu      600

uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga gucagcaaua      660

gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug gaacauggag      720

guugugucac cguaauggca caggacaaac cgacugucga cauagagcug guuacaacaa      780

cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua ucagacaugg      840

cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa ucagacacuc      900

aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu ggacuuuuug      960

gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga     1020

gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc ucccagcaca     1080

gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg aagguugaga     1140

uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc cuaggacuug     1200

auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu augaauaaca     1260

agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg cacgcugggg     1320

cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc aaggacgcac     1380

augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu cacacggccc     1440

uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc ucuggccacu     1500

ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac uccuugugua     1560

ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca gucacagugg     1620

agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug gcgguggaca     1680

ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc acugaaagca     1740

cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu uacauuguca     1800

uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc accauuggaa     1860

aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga gacacagccu     1920

gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc caucaaauuu     1980

uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa auucucauug     2040

gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc cuuaugugcu     2100

uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau guggggugcu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5274
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5274
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagag acggggcgca gauaccagug ucggaauugu uggccuccug cugaccacag      120

cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug gacagaaacg      180

augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu uauauacaga      240

ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu augcuggaug      300

agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu uggguugugu      360

acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu gugacgcucc      420

ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau      480

acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc uucgcguuag      540

cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg      600

ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc agcaauaggg      660

acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa cauggagguu      720

gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu acaacaacag      780

ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca gacauggcuu      840

cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca gacacucaau      900

augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga cuuuuuggca      960

aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc gggaagagca     1020

uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc cagcacagug     1080

ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag guugagauaa     1140

cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua ggacuugauu     1200

gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug aauaacaagc     1260

acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac gcuggggcag     1320

acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag gacgcacaug     1380

ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac acggcccuug     1440

cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu ggccacuuga     1500

aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc uuguguaccg     1560

cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc acaguggagg     1620

uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg guggacaugc     1680

aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu gaaagcacug     1740

agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac auugucauag     1800

gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc auuggaaaag     1860

cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac acagccuggg     1920

acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau caaauuuuug     1980

gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu cucauuggaa     2040

cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu augugcuugg     2100

ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugaugug gggugcugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5275
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5275
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagagacg gggcgcagau accagugucg gaauuguugg ccuccugcug accacagcua      120

uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac agaaacgaug      180

cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau auacagauca      240

uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug cuggaugagg      300

ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg guuguguacg      360

gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug acgcuccccu      420

cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca agagaauaca      480

caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc gcguuagcag      540

cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua uacuugguca      600

ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc aauagggacu      660

uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau ggagguugug      720

ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca acaacaguca      780

gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac auggcuucgg      840

acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac acucaauaug      900

ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu uuuggcaaag      960

ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg aagagcaucc     1020

agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag cacaguggga     1080

ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu gagauaacgc     1140

ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga cuugauugug     1200

aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau aacaagcacu     1260

gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu ggggcagaca     1320

ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac gcacaugcca     1380

aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg gcccuugcug     1440

gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc cacuugaaau     1500

gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug uguaccgcag     1560

cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca guggagguac     1620

aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug gacaugcaaa     1680

cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa agcacugaga     1740

acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu gucauaggag     1800

ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu ggaaaagcau     1860

uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca gccugggacu     1920

uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa auuuuuggag     1980

cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc auuggaacgu     2040

ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug ugcuuggccu     2100

uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg ugcugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5276
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5276
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agagacgggg cgcagauacc agugucggaa uuguuggccu ccugcugacc acagcuaugg      120

cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga gguuguguca      720

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu     1260

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1860

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1920

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu uuuggagcag     1980

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu ggaacguugc     2040

ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     2100

ggggaguguu gaucuucuua uccacagccg ucucugcuga uguggggugc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5277
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5277
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gacggggcgc agauaccagu gucggaauug uuggccuccu gcugaccaca gcuauggcag      120

cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu ugugucaccg      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggaguc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1260

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     1860

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     1920

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu ggagcagcuu     1980

ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga acguugcuga     2040

ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2100

gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5278
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5278
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgcaga uaccaguguc ggaauuguug gccuccugcu gaccacagcu auggcagcgg      120

aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau gcuggggagg      180

ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc auggaucuug      240

gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag gggguggaac      300

cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac ggaaccugcc      360

aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc ucccauucca      420

cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac acaaagcacu      480

ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca gcagcugcca      540

ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc augauacugc      600

ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac uuuguggaag      660

guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu gucaccguaa      720

uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc agcaacaugg      780

cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg gacagccgcu      840

gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau gucugcaaaa      900

gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa gggagucugg      960

ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc cagccagaga     1020

aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg augaucguua     1080

augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg cccaauucac     1140

caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu gaaccgagga     1200

caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac ugguugguuc     1260

acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac accggaacuc     1320

cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc aaaaggcaaa     1380

cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu ggagcucugg     1440

aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa ugucgccuga     1500

aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca gcguucacau     1560

ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua caguacgcag     1620

ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa acucugaccc     1680

caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag aacucuaaga     1740

ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga gucggggaga     1800

agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca uuugaagcca     1860

cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac uuuggaucag     1920

uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga gcagcuuuca     1980

aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg uugcugaugu     2040

gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc uuagggggag     2100

uguugaucuu cuuauccaca gccgucucug cugauguggg gugcugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5279
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5279
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug gcagcggagg      120

ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu ggggaggcca      180

uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug gaucuuggac      240

acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg guggaaccag      300

augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga accugccauc      360

acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc cauuccacua      420

ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca aagcacuuga      480

uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca gcugccaucg      540

cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug auacugcuga      600

uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu guggaaggua      660

ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc accguaaugg      720

cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc aacauggcgg      780

agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac agccgcugcc      840

caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc ugcaaaagaa      900

cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg agucugguga      960

caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag ccagagaauc     1020

uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug aucguuaaug     1080

acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc aauucaccaa     1140

gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa ccgaggacag     1200

gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg uugguucaca     1260

aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac     1320

acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa aggcaaacug     1380

ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga gcucuggagg     1440

cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa     1500

uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg uucacauuca     1560

ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag uacgcaggga     1620

cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu cugaccccag     1680

uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac ucuaagauga     1740

ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc ggggagaaga     1800

agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu gaagccacug     1860

ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu ggaucaguug     1920

gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca gcuuucaaau     1980

cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug cugauguggu     2040

ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua gggggagugu     2100

ugaucuucuu auccacagcc gucucugcug auguggggug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5280
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5280
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

cagauaccag ugucggaauu guuggccucc ugcugaccac agcuauggca gcggagguca      120

cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg gaggccauau      180

cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau cuuggacaca      240

ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug gaaccagaug      300

acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc ugccaucaca      360

aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau uccacuagga      420

agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag cacuugauua      480

gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu gccaucgcuu      540

ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua cugcugauug      600

ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug gaagguaugu      660

caggugggac uuggguugau auugucuugg aacauggagg uugugucacc guaauggcac      720

aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac auggcggagg      780

uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc cgcugcccaa      840

cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc aaaagaacgu      900

uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu cuggugacau      960

gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca gagaaucugg     1020

aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc guuaaugaca     1080

caggacauga aacugaugag aauagagcga agguugagau aacgcccaau ucaccaagag     1140

ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg aggacaggcc     1200

uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug guucacaagg     1260

agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga acuccacacu     1320

ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg caaacugucg     1380

ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu cuggaggcug     1440

agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg     1500

auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc acauucacca     1560

agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac gcagggacag     1620

auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug accccaguug     1680

ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu aagaugaugc     1740

uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg gagaagaaga     1800

ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa gccacuguga     1860

gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga ucaguuggag     1920

gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu uucaaaucau     1980

uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug augugguugg     2040

gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga     2100

ucuucuuauc cacagccguc ucugcugaug uggggugcug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5281
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5281
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcag       60

auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg gaggucacua      120

gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag gccauaucuu      180

uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu ggacacaugu      240

gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa ccagaugacg      300

ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc caucacaaaa      360

aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc acuaggaagc      420

ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac uugauuagag      480

ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc      540

uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug cugauugccc      600

cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa gguaugucag      660

gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua auggcacagg      720

acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug gcggagguaa      780

gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc ugcccaacac      840

aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa agaacguuag      900

uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug gugacaugcg      960

cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag aaucuggagu     1020

accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu aaugacacag     1080

gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca ccaagagccg     1140

aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg acaggccuug     1200

acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu cacaaggagu     1260

gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu ccacacugga     1320

acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa acugucgugg     1380

uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug gaggcugaga     1440

uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug aaaauggaua     1500

aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca uucaccaaga     1560

ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca gggacagaug     1620

gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc ccaguuggga     1680

gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag augaugcugg     1740

aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag aagaagauca     1800

cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc acugugagag     1860

gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca guuggaggcg     1920

cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu     1980

uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug ugguuggguc     2040

ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga guguugaucu     2100

ucuuauccac agccgucucu gcugaugugg ggugcugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5282
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5282
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaua       60

ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag gucacuagac      120

gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc      180

caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga cacaugugug      240

augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca gaugacgucg      300

auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau cacaaaaaag      360

gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu aggaagcugc      420

aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug auuagagucg      480

aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu      540

ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug auugccccgg      600

cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu augucaggug      660

ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug gcacaggaca      720

aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg gagguaagau      780

ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc ccaacacaag      840

gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga acguuagugg      900

acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug acaugcgcua      960

aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau cuggaguacc     1020

ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau gacacaggac     1080

augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca agagccgaag     1140

ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca ggccuugacu     1200

uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac aaggaguggu     1260

uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca cacuggaaca     1320

acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu gucgugguuc     1380

uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag gcugagaugg     1440

auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa auggauaaac     1500

uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc accaagaucc     1560

cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg acagauggac     1620

cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca guugggaggu     1680

ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug augcuggaac     1740

uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag aagaucaccc     1800

accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu gugagaggug     1860

ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu ggaggcgcuc     1920

ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug     1980

gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg uugggucuga     2040

acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug uugaucuucu     2100

uauccacagc cgucucugcu gauguggggu gcugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5283
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5283
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcugau guggggugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5284
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5284
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggccguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcugau guggggugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5285
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5285
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug gcagcggccg      120

ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu ggggaggcca      180

uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug gaucuuggac      240

acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg guggaaccag      300

augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga accugccauc      360

acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc cauuccacua      420

ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca aagcacuuga      480

uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca gcugccaucg      540

cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug auacugcuga      600

uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu guggaaggua      660

ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc accguaaugg      720

cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc aacauggcgg      780

agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac agccgcugcc      840

caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc ugcaaaagaa      900

cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg agucugguga      960

caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag ccagagaauc     1020

uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug aucguuaaug     1080

acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc aauucaccaa     1140

gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa ccgaggacag     1200

gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg uugguucaca     1260

aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac     1320

acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa aggcaaacug     1380

ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga gcucuggagg     1440

cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa     1500

uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg uucacauuca     1560

ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag uacgcaggga     1620

cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu cugaccccag     1680

uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac ucuaagauga     1740

ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc ggggagaaga     1800

agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu gaagccacug     1860

ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu ggaucaguug     1920

gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca gcuuucaaau     1980

cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug cugauguggu     2040

ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua gggggagugu     2100

ugaucuucuu auccacagcc gucucugcug auguggggug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5286
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5286
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuguccuc aaggaaacgc cguucccaug auguucugac ugugcaauuc cuaauuuugg      120

gaaugcuguu gaugacgggu ggagugaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5287
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5287
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuguccuc aaggaaacgc cguucccaug auguucugac ugugcaauuc cuaauuuugg      120

gaaugcuguu gaugacgggu gccgugaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5288
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5288
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac ggggcgcaga uaccaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5289
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5289
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

ucaaugcuag gaaggagaag aagagacggg gcgcagauac cagugucgga auuguuggcc      120

uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca uacuauaugu      180

acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug gggaugaaua      240

aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug agcuaugaau      300

gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc aacacgacgu      360

caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg agaucuagaa      420

gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg caaaccuggu      480

uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua uucaggaacc      540

cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa      600

aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc aggugcauag      660

gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu gauauugucu      720

uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc gacauagagc      780

ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau gaggcaucaa      840

uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac cuugacaagc      900

aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg ggaaauggau      960

guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc uccaagaaaa     1020

ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug ucaguucaug     1080

gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau gagaauagag     1140

cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg gguuuuggaa     1200

gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug uauuacuuga     1260

cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu ccauuaccuu     1320

ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca cugguagagu     1380

ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa gaaggagcag     1440

uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag ggaaggcugu     1500

ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag ggcgugucau     1560

acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca cugcacggga     1620

cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu ccagcucaga     1680

uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu aaccccguaa     1740

ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca uuuggggacu     1800

cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac aggaguggca     1860

gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug gcagucuugg     1920

gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug ggcaagggca     1980

uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc ugguucucac     2040

aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau ggaucuauuu     2100

cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc gucucugcug     2160

auugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5290
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5290
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu guuggccucc      120

ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac uauauguacu      180

uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg augaauaagu      240

guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc uaugaaugcc      300

cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac acgacgucaa      360

cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga ucuagaagag      420

cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa accugguugg      480

aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc aggaacccug      540

gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag      600

ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg ugcauaggag      660

ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau auugucuugg      720

aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac auagagcugg      780

uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag gcaucaauau      840

cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu gacaagcaau      900

cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga aauggaugug      960

gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga     1020

ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca guucauggcu     1080

cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag aauagagcga     1140

agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu uuuggaagcc     1200

uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau uacuugacua     1260

ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca uuaccuuggc     1320

acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug guagaguuca     1380

aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa ggagcaguuc     1440

acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga aggcuguccu     1500

cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc gugucauacu     1560

ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug cacgggacag     1620

ucacagugga gguacaguac gcagggacag auggaccuug caagguucca gcucagaugg     1680

cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac cccguaauca     1740

cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu ggggacucuu     1800

acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg aguggcagca     1860

ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca gucuugggag     1920

acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc aagggcaucc     1980

aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg uucucacaaa     2040

uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga ucuauuuccc     2100

uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc ucugcugauu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5291
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5291
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaug       60

cuaggaagga gaagaagaga cggggcgcag auaccagugu cggaauuguu ggccuccugc      120

ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau auguacuugg      180

acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug aauaaguguu      240

auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau gaaugcccua      300

ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg acgucaacuu      360

ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu agaagagcug      420

ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau      480

caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg aacccuggcu      540

ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc caaaaaguca      600

uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc auaggaguca      660

gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu gucuuggaac      720

auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua gagcugguua      780

caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca ucaauaucag      840

acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac aagcaaucag      900

acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau ggauguggac      960

uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag aaaaugaccg     1020

ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu cauggcuccc     1080

agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau agagcgaagg     1140

uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu ggaagccuag     1200

gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac uugacuauga     1260

auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua ccuuggcacg     1320

cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua gaguucaagg     1380

acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga gcaguucaca     1440

cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg cuguccucug     1500

gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug ucauacuccu     1560

uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac gggacaguca     1620

caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu cagauggcgg     1680

uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc guaaucacug     1740

aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg gacucuuaca     1800

uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu ggcagcacca     1860

uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc uugggagaca     1920

cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag ggcauccauc     1980

aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc ucacaaauuc     2040

ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu auuucccuua     2100

ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu gcugauugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5292
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5292
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcua       60

ggaaggagaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc cuccugcuga      120

ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug uacuuggaca      180

gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau aaguguuaua      240

uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa ugcccuaugc      300

uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg ucaacuuggg      360

uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga agagcuguga      420

cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa      480

gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac ccuggcuucg      540

cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau      600

acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua ggagucagca      660

auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc uuggaacaug      720

gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag cugguuacaa      780

caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca auaucagaca      840

uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag caaucagaca      900

cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga uguggacuuu      960

uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa augaccggga     1020

agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau ggcucccagc     1080

acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga gcgaagguug     1140

agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga agccuaggac     1200

uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug acuaugaaua     1260

acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu uggcacgcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag uucaaggacg     1380

cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca guucacacgg     1440

cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug uccucuggcc     1500

acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca uacuccuugu     1560

guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg acagucacag     1620

uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag auggcggugg     1680

acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua aucacugaaa     1740

gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac ucuuacauug     1800

ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc agcaccauug     1860

gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug ggagacacag     1920

ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa     1980

uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca caaauucuca     2040

uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu ucccuuaugu     2100

gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu gauugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5293
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5293
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

aggagaagaa gagacggggc gcagauacca gugucggaau uguuggccuc cugcugacca      120

cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac uuggacagaa      180

acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag uguuauauac      240

agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc ccuaugcugg      300

augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca acuuggguug      360

uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga gcugugacgc      420

uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug gaaucaagag      480

aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu ggcuucgcgu      540

uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa gucauauacu      600

uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga gucagcaaua      660

gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug gaacauggag      720

guugugucac cguaauggca caggacaaac cgacugucga cauagagcug guuacaacaa      780

cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua ucagacaugg      840

cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa ucagacacuc      900

aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu ggacuuuuug      960

gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga     1020

gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc ucccagcaca     1080

gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg aagguugaga     1140

uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc cuaggacuug     1200

auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu augaauaaca     1260

agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg cacgcugggg     1320

cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc aaggacgcac     1380

augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu cacacggccc     1440

uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc ucuggccacu     1500

ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac uccuugugua     1560

ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca gucacagugg     1620

agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug gcgguggaca     1680

ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc acugaaagca     1740

cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu uacauuguca     1800

uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc accauuggaa     1860

aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga gacacagccu     1920

gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc caucaaauuu     1980

uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa auucucauug     2040

gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc cuuaugugcu     2100

uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5294
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5294
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagag acggggcgca gauaccagug ucggaauugu uggccuccug cugaccacag      120

cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug gacagaaacg      180

augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu uauauacaga      240

ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu augcuggaug      300

agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu uggguugugu      360

acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu gugacgcucc      420

ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau      480

acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc uucgcguuag      540

cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg      600

ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc agcaauaggg      660

acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa cauggagguu      720

gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu acaacaacag      780

ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca gacauggcuu      840

cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca gacacucaau      900

augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga cuuuuuggca      960

aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc gggaagagca     1020

uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc cagcacagug     1080

ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag guugagauaa     1140

cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua ggacuugauu     1200

gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug aauaacaagc     1260

acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac gcuggggcag     1320

acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag gacgcacaug     1380

ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac acggcccuug     1440

cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu ggccacuuga     1500

aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc uuguguaccg     1560

cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc acaguggagg     1620

uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg guggacaugc     1680

aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu gaaagcacug     1740

agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac auugucauag     1800

gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc auuggaaaag     1860

cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac acagccuggg     1920

acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau caaauuuuug     1980

gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu cucauuggaa     2040

cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu augugcuugg     2100

ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugauuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5295
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5295
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagagacg gggcgcagau accagugucg gaauuguugg ccuccugcug accacagcua      120

uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac agaaacgaug      180

cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau auacagauca      240

uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug cuggaugagg      300

ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg guuguguacg      360

gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug acgcuccccu      420

cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca agagaauaca      480

caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc gcguuagcag      540

cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua uacuugguca      600

ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc aauagggacu      660

uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau ggagguugug      720

ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca acaacaguca      780

gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac auggcuucgg      840

acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac acucaauaug      900

ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu uuuggcaaag      960

ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg aagagcaucc     1020

agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag cacaguggga     1080

ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu gagauaacgc     1140

ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga cuugauugug     1200

aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau aacaagcacu     1260

gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu ggggcagaca     1320

ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac gcacaugcca     1380

aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg gcccuugcug     1440

gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc cacuugaaau     1500

gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug uguaccgcag     1560

cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca guggagguac     1620

aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug gacaugcaaa     1680

cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa agcacugaga     1740

acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu gucauaggag     1800

ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu ggaaaagcau     1860

uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca gccugggacu     1920

uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa auuuuuggag     1980

cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc auuggaacgu     2040

ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug ugcuuggccu     2100

uagggggagu guugaucuuc uuauccacag ccgucucugc ugauugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5296
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5296
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agagacgggg cgcagauacc agugucggaa uuguuggccu ccugcugacc acagcuaugg      120

cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga gguuguguca      720

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu     1260

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1860

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1920

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu uuuggagcag     1980

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu ggaacguugc     2040

ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     2100

ggggaguguu gaucuucuua uccacagccg ucucugcuga uugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5297
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5297
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gacggggcgc agauaccagu gucggaauug uuggccuccu gcugaccaca gcuauggcag      120

cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu ugugucaccg      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggaguc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1260

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     1860

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     1920

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu ggagcagcuu     1980

ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga acguugcuga     2040

ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2100

gaguguugau cuucuuaucc acagccgucu cugcugauug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5298
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5298
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgcaga uaccaguguc ggaauuguug gccuccugcu gaccacagcu auggcagcgg      120

aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau gcuggggagg      180

ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc auggaucuug      240

gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag gggguggaac      300

cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac ggaaccugcc      360

aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc ucccauucca      420

cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac acaaagcacu      480

ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca gcagcugcca      540

ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc augauacugc      600

ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac uuuguggaag      660

guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu gucaccguaa      720

uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc agcaacaugg      780

cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg gacagccgcu      840

gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau gucugcaaaa      900

gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa gggagucugg      960

ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc cagccagaga     1020

aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg augaucguua     1080

augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg cccaauucac     1140

caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu gaaccgagga     1200

caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac ugguugguuc     1260

acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac accggaacuc     1320

cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc aaaaggcaaa     1380

cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu ggagcucugg     1440

aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa ugucgccuga     1500

aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca gcguucacau     1560

ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua caguacgcag     1620

ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa acucugaccc     1680

caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag aacucuaaga     1740

ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga gucggggaga     1800

agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca uuugaagcca     1860

cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac uuuggaucag     1920

uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga gcagcuuuca     1980

aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg uugcugaugu     2040

gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc uuagggggag     2100

uguugaucuu cuuauccaca gccgucucug cugauugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5299
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5299
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug gcagcggagg      120

ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu ggggaggcca      180

uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug gaucuuggac      240

acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg guggaaccag      300

augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga accugccauc      360

acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc cauuccacua      420

ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca aagcacuuga      480

uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca gcugccaucg      540

cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug auacugcuga      600

uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu guggaaggua      660

ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc accguaaugg      720

cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc aacauggcgg      780

agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac agccgcugcc      840

caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc ugcaaaagaa      900

cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg agucugguga      960

caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag ccagagaauc     1020

uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug aucguuaaug     1080

acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc aauucaccaa     1140

gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa ccgaggacag     1200

gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg uugguucaca     1260

aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac     1320

acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa aggcaaacug     1380

ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga gcucuggagg     1440

cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa     1500

uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg uucacauuca     1560

ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag uacgcaggga     1620

cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu cugaccccag     1680

uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac ucuaagauga     1740

ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc ggggagaaga     1800

agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu gaagccacug     1860

ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu ggaucaguug     1920

gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca gcuuucaaau     1980

cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug cugauguggu     2040

ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua gggggagugu     2100

ugaucuucuu auccacagcc gucucugcug auugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5300
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5300
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

cagauaccag ugucggaauu guuggccucc ugcugaccac agcuauggca gcggagguca      120

cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg gaggccauau      180

cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau cuuggacaca      240

ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug gaaccagaug      300

acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc ugccaucaca      360

aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau uccacuagga      420

agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag cacuugauua      480

gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu gccaucgcuu      540

ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua cugcugauug      600

ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug gaagguaugu      660

caggugggac uuggguugau auugucuugg aacauggagg uugugucacc guaauggcac      720

aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac auggcggagg      780

uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc cgcugcccaa      840

cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc aaaagaacgu      900

uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu cuggugacau      960

gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca gagaaucugg     1020

aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc guuaaugaca     1080

caggacauga aacugaugag aauagagcga agguugagau aacgcccaau ucaccaagag     1140

ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg aggacaggcc     1200

uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug guucacaagg     1260

agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga acuccacacu     1320

ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg caaacugucg     1380

ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu cuggaggcug     1440

agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg     1500

auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc acauucacca     1560

agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac gcagggacag     1620

auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug accccaguug     1680

ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu aagaugaugc     1740

uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg gagaagaaga     1800

ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa gccacuguga     1860

gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga ucaguuggag     1920

gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu uucaaaucau     1980

uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug augugguugg     2040

gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga     2100

ucuucuuauc cacagccguc ucugcugauu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5301
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5301
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcag       60

auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg gaggucacua      120

gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag gccauaucuu      180

uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu ggacacaugu      240

gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa ccagaugacg      300

ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc caucacaaaa      360

aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc acuaggaagc      420

ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac uugauuagag      480

ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc      540

uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug cugauugccc      600

cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa gguaugucag      660

gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua auggcacagg      720

acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug gcggagguaa      780

gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc ugcccaacac      840

aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa agaacguuag      900

uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug gugacaugcg      960

cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag aaucuggagu     1020

accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu aaugacacag     1080

gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca ccaagagccg     1140

aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg acaggccuug     1200

acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu cacaaggagu     1260

gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu ccacacugga     1320

acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa acugucgugg     1380

uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug gaggcugaga     1440

uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug aaaauggaua     1500

aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca uucaccaaga     1560

ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca gggacagaug     1620

gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc ccaguuggga     1680

gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag augaugcugg     1740

aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag aagaagauca     1800

cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc acugugagag     1860

gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca guuggaggcg     1920

cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu     1980

uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug ugguuggguc     2040

ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga guguugaucu     2100

ucuuauccac agccgucucu gcugauugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  5302
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5302
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaua       60

ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag gucacuagac      120

gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc      180

caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga cacaugugug      240

augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca gaugacgucg      300

auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau cacaaaaaag      360

gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu aggaagcugc      420

aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug auuagagucg      480

aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu      540

ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug auugccccgg      600

cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu augucaggug      660

ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug gcacaggaca      720

aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg gagguaagau      780

ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc ccaacacaag      840

gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga acguuagugg      900

acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug acaugcgcua      960

aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau cuggaguacc     1020

ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau gacacaggac     1080

augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca agagccgaag     1140

ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca ggccuugacu     1200

uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac aaggaguggu     1260

uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca cacuggaaca     1320

acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu gucgugguuc     1380

uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag gcugagaugg     1440

auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa auggauaaac     1500

uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc accaagaucc     1560

cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg acagauggac     1620

cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca guugggaggu     1680

ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug augcuggaac     1740

uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag aagaucaccc     1800

accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu gugagaggug     1860

ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu ggaggcgcuc     1920

ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug     1980

gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg uugggucuga     2040

acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug uugaucuucu     2100

uauccacagc cgucucugcu gauugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  5303
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5303
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcugau ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5304
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5304
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggccguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcugau ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5305
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5305
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug gcagcggccg      120

ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu ggggaggcca      180

uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug gaucuuggac      240

acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg guggaaccag      300

augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga accugccauc      360

acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc cauuccacua      420

ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca aagcacuuga      480

uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca gcugccaucg      540

cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug auacugcuga      600

uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu guggaaggua      660

ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc accguaaugg      720

cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc aacauggcgg      780

agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac agccgcugcc      840

caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc ugcaaaagaa      900

cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg agucugguga      960

caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag ccagagaauc     1020

uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug aucguuaaug     1080

acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc aauucaccaa     1140

gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa ccgaggacag     1200

gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg uugguucaca     1260

aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac     1320

acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa aggcaaacug     1380

ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga gcucuggagg     1440

cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa     1500

uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg uucacauuca     1560

ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag uacgcaggga     1620

cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu cugaccccag     1680

uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac ucuaagauga     1740

ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc ggggagaaga     1800

agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu gaagccacug     1860

ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu ggaucaguug     1920

gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca gcuuucaaau     1980

cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug cugauguggu     2040

ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua gggggagugu     2100

ugaucuucuu auccacagcc gucucugcug auugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5306
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5306
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuguccuc aaggaaacgc cguucccaug auguucugac ugugcaauuc cuaauuuugg      120

gaaugcuguu gaugacgggu ggagugaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5307
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5307
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuguccuc aaggaaacgc cguucccaug auguucugac ugugcaauuc cuaauuuugg      120

gaaugcuguu gaugacgggu gccgugaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5308
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5308
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac ggggcgcaga uaccaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5309
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5309
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

augcuaggaa ggagaagaag agacggggcg cagauaccag ugucggaauu guuggccucc      120

ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac uauauguacu      180

uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg augaauaagu      240

guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc uaugaaugcc      300

cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac acgacgucaa      360

cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga ucuagaagag      420

cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa accugguugg      480

aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc aggaacccug      540

gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag      600

ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg ugcauaggag      660

ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau auugucuugg      720

aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac auagagcugg      780

uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag gcaucaauau      840

cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu gacaagcaau      900

cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga aauggaugug      960

gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga     1020

ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca guucauggcu     1080

cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag aauagagcga     1140

agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu uuuggaagcc     1200

uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau uacuugacua     1260

ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca uuaccuuggc     1320

acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug guagaguuca     1380

aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa ggagcaguuc     1440

acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga aggcuguccu     1500

cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc gugucauacu     1560

ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug cacgggacag     1620

ucacagugga gguacaguac gcagggacag auggaccuug caagguucca gcucagaugg     1680

cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac cccguaauca     1740

cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu ggggacucuu     1800

acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg aguggcagca     1860

ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca gucuugggag     1920

acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc aagggcaucc     1980

aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg uucucacaaa     2040

uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga ucuauuuccc     2100

uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc ucugcuugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5310
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5310
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

ucaaugcuag gaaggagaag aagagacggg gcgcagauac cagugucgga auuguuggcc      120

uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca uacuauaugu      180

acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug gggaugaaua      240

aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug agcuaugaau      300

gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc aacacgacgu      360

caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg agaucuagaa      420

gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg caaaccuggu      480

uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua uucaggaacc      540

cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa      600

aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc aggugcauag      660

gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu gauauugucu      720

uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc gacauagagc      780

ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau gaggcaucaa      840

uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac cuugacaagc      900

aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg ggaaauggau      960

guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc uccaagaaaa     1020

ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug ucaguucaug     1080

gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau gagaauagag     1140

cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg gguuuuggaa     1200

gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug uauuacuuga     1260

cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu ccauuaccuu     1320

ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca cugguagagu     1380

ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa gaaggagcag     1440

uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag ggaaggcugu     1500

ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag ggcgugucau     1560

acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca cugcacggga     1620

cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu ccagcucaga     1680

uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu aaccccguaa     1740

ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca uuuggggacu     1800

cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac aggaguggca     1860

gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug gcagucuugg     1920

gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug ggcaagggca     1980

uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc ugguucucac     2040

aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau ggaucuauuu     2100

cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc gucucugcuu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5311
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5311
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaug       60

cuaggaagga gaagaagaga cggggcgcag auaccagugu cggaauuguu ggccuccugc      120

ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau auguacuugg      180

acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug aauaaguguu      240

auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau gaaugcccua      300

ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg acgucaacuu      360

ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu agaagagcug      420

ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc ugguuggaau      480

caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg aacccuggcu      540

ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc caaaaaguca      600

uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc auaggaguca      660

gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu gucuuggaac      720

auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua gagcugguua      780

caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca ucaauaucag      840

acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac aagcaaucag      900

acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau ggauguggac      960

uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag aaaaugaccg     1020

ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu cauggcuccc     1080

agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau agagcgaagg     1140

uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu ggaagccuag     1200

gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac uugacuauga     1260

auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua ccuuggcacg     1320

cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua gaguucaagg     1380

acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga gcaguucaca     1440

cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg cuguccucug     1500

gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug ucauacuccu     1560

uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac gggacaguca     1620

caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu cagauggcgg     1680

uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc guaaucacug     1740

aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg gacucuuaca     1800

uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu ggcagcacca     1860

uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc uugggagaca     1920

cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag ggcauccauc     1980

aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc ucacaaauuc     2040

ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu auuucccuua     2100

ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu gcuugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5312
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5312
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcua       60

ggaaggagaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc cuccugcuga      120

ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug uacuuggaca      180

gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau aaguguuaua      240

uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa ugcccuaugc      300

uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg ucaacuuggg      360

uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga agagcuguga      420

cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg uuggaaucaa      480

gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac ccuggcuucg      540

cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa aaagucauau      600

acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua ggagucagca      660

auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc uuggaacaug      720

gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag cugguuacaa      780

caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca auaucagaca      840

uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag caaucagaca      900

cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga uguggacuuu      960

uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa augaccggga     1020

agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau ggcucccagc     1080

acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga gcgaagguug     1140

agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga agccuaggac     1200

uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug acuaugaaua     1260

acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu uggcacgcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag uucaaggacg     1380

cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca guucacacgg     1440

cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug uccucuggcc     1500

acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca uacuccuugu     1560

guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg acagucacag     1620

uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag auggcggugg     1680

acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua aucacugaaa     1740

gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac ucuuacauug     1800

ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc agcaccauug     1860

gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug ggagacacag     1920

ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc auccaucaaa     1980

uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca caaauucuca     2040

uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu ucccuuaugu     2100

gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5313
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5313
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

aggagaagaa gagacggggc gcagauacca gugucggaau uguuggccuc cugcugacca      120

cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac uuggacagaa      180

acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag uguuauauac      240

agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc ccuaugcugg      300

augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca acuuggguug      360

uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga gcugugacgc      420

uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug gaaucaagag      480

aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu ggcuucgcgu      540

uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa gucauauacu      600

uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga gucagcaaua      660

gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug gaacauggag      720

guugugucac cguaauggca caggacaaac cgacugucga cauagagcug guuacaacaa      780

cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua ucagacaugg      840

cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa ucagacacuc      900

aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu ggacuuuuug      960

gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga     1020

gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc ucccagcaca     1080

gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg aagguugaga     1140

uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc cuaggacuug     1200

auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu augaauaaca     1260

agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg cacgcugggg     1320

cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc aaggacgcac     1380

augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu cacacggccc     1440

uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc ucuggccacu     1500

ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac uccuugugua     1560

ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca gucacagugg     1620

agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug gcgguggaca     1680

ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc acugaaagca     1740

cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu uacauuguca     1800

uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc accauuggaa     1860

aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga gacacagccu     1920

gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc caucaaauuu     1980

uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa auucucauug     2040

gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc cuuaugugcu     2100

uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcuuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5314
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5314
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagag acggggcgca gauaccagug ucggaauugu uggccuccug cugaccacag      120

cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug gacagaaacg      180

augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu uauauacaga      240

ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu augcuggaug      300

agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu uggguugugu      360

acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu gugacgcucc      420

ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau      480

acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc uucgcguuag      540

cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg      600

ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc agcaauaggg      660

acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa cauggagguu      720

gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu acaacaacag      780

ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca gacauggcuu      840

cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca gacacucaau      900

augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga cuuuuuggca      960

aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc gggaagagca     1020

uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc cagcacagug     1080

ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag guugagauaa     1140

cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua ggacuugauu     1200

gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug aauaacaagc     1260

acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac gcuggggcag     1320

acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag gacgcacaug     1380

ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac acggcccuug     1440

cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu ggccacuuga     1500

aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc uuguguaccg     1560

cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc acaguggagg     1620

uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg guggacaugc     1680

aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu gaaagcacug     1740

agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac auugucauag     1800

gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc auuggaaaag     1860

cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac acagccuggg     1920

acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau caaauuuuug     1980

gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu cucauuggaa     2040

cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu augugcuugg     2100

ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcuugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5315
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5315
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagagacg gggcgcagau accagugucg gaauuguugg ccuccugcug accacagcua      120

uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac agaaacgaug      180

cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau auacagauca      240

uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug cuggaugagg      300

ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg guuguguacg      360

gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug acgcuccccu      420

cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca agagaauaca      480

caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc gcguuagcag      540

cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua uacuugguca      600

ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc aauagggacu      660

uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau ggagguugug      720

ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca acaacaguca      780

gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac auggcuucgg      840

acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac acucaauaug      900

ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu uuuggcaaag      960

ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg aagagcaucc     1020

agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag cacaguggga     1080

ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu gagauaacgc     1140

ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga cuugauugug     1200

aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau aacaagcacu     1260

gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu ggggcagaca     1320

ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac gcacaugcca     1380

aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg gcccuugcug     1440

gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc cacuugaaau     1500

gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug uguaccgcag     1560

cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca guggagguac     1620

aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug gacaugcaaa     1680

cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa agcacugaga     1740

acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu gucauaggag     1800

ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu ggaaaagcau     1860

uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca gccugggacu     1920

uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa auuuuuggag     1980

cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc auuggaacgu     2040

ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug ugcuuggccu     2100

uagggggagu guugaucuuc uuauccacag ccgucucugc uugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5316
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5316
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agagacgggg cgcagauacc agugucggaa uuguuggccu ccugcugacc acagcuaugg      120

cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga gguuguguca      720

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu     1260

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1860

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1920

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu uuuggagcag     1980

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu ggaacguugc     2040

ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     2100

ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5317
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5317
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gacggggcgc agauaccagu gucggaauug uuggccuccu gcugaccaca gcuauggcag      120

cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu ugugucaccg      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggaguc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1260

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     1860

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     1920

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu ggagcagcuu     1980

ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga acguugcuga     2040

ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2100

gaguguugau cuucuuaucc acagccgucu cugcuugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5318
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5318
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgcaga uaccaguguc ggaauuguug gccuccugcu gaccacagcu auggcagcgg      120

aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau gcuggggagg      180

ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc auggaucuug      240

gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag gggguggaac      300

cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac ggaaccugcc      360

aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc ucccauucca      420

cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac acaaagcacu      480

ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca gcagcugcca      540

ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc augauacugc      600

ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac uuuguggaag      660

guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu gucaccguaa      720

uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc agcaacaugg      780

cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg gacagccgcu      840

gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau gucugcaaaa      900

gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa gggagucugg      960

ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc cagccagaga     1020

aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg augaucguua     1080

augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg cccaauucac     1140

caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu gaaccgagga     1200

caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac ugguugguuc     1260

acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac accggaacuc     1320

cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc aaaaggcaaa     1380

cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu ggagcucugg     1440

aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa ugucgccuga     1500

aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca gcguucacau     1560

ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua caguacgcag     1620

ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa acucugaccc     1680

caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag aacucuaaga     1740

ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga gucggggaga     1800

agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca uuugaagcca     1860

cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac uuuggaucag     1920

uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga gcagcuuuca     1980

aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg uugcugaugu     2040

gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc uuagggggag     2100

uguugaucuu cuuauccaca gccgucucug cuugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5319
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5319
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug gcagcggagg      120

ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu ggggaggcca      180

uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug gaucuuggac      240

acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg guggaaccag      300

augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga accugccauc      360

acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc cauuccacua      420

ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca aagcacuuga      480

uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca gcugccaucg      540

cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug auacugcuga      600

uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu guggaaggua      660

ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc accguaaugg      720

cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc aacauggcgg      780

agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac agccgcugcc      840

caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc ugcaaaagaa      900

cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg agucugguga      960

caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag ccagagaauc     1020

uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug aucguuaaug     1080

acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc aauucaccaa     1140

gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa ccgaggacag     1200

gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg uugguucaca     1260

aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac     1320

acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa aggcaaacug     1380

ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga gcucuggagg     1440

cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa     1500

uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg uucacauuca     1560

ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag uacgcaggga     1620

cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu cugaccccag     1680

uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac ucuaagauga     1740

ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc ggggagaaga     1800

agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu gaagccacug     1860

ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu ggaucaguug     1920

gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca gcuuucaaau     1980

cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug cugauguggu     2040

ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua gggggagugu     2100

ugaucuucuu auccacagcc gucucugcuu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5320
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5320
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

cagauaccag ugucggaauu guuggccucc ugcugaccac agcuauggca gcggagguca      120

cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg gaggccauau      180

cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau cuuggacaca      240

ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug gaaccagaug      300

acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc ugccaucaca      360

aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau uccacuagga      420

agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag cacuugauua      480

gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu gccaucgcuu      540

ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua cugcugauug      600

ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug gaagguaugu      660

caggugggac uuggguugau auugucuugg aacauggagg uugugucacc guaauggcac      720

aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac auggcggagg      780

uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc cgcugcccaa      840

cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc aaaagaacgu      900

uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagu cuggugacau      960

gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca gagaaucugg     1020

aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc guuaaugaca     1080

caggacauga aacugaugag aauagagcga agguugagau aacgcccaau ucaccaagag     1140

ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg aggacaggcc     1200

uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug guucacaagg     1260

agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga acuccacacu     1320

ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg caaacugucg     1380

ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu cuggaggcug     1440

agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg     1500

auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc acauucacca     1560

agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac gcagggacag     1620

auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug accccaguug     1680

ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu aagaugaugc     1740

uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg gagaagaaga     1800

ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa gccacuguga     1860

gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga ucaguuggag     1920

gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu uucaaaucau     1980

uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug augugguugg     2040

gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga     2100

ucuucuuauc cacagccguc ucugcuugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  5321
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5321
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcag       60

auaccagugu cggaauuguu ggccuccugc ugaccacagc uauggcagcg gaggucacua      120

gacgugggag ugcauacuau auguacuugg acagaaacga ugcuggggag gccauaucuu      180

uuccaaccac auuggggaug aauaaguguu auauacagau cauggaucuu ggacacaugu      240

gugaugccac caugagcuau gaaugcccua ugcuggauga ggggguggaa ccagaugacg      300

ucgauuguug gugcaacacg acgucaacuu ggguugugua cggaaccugc caucacaaaa      360

aaggugaagc acggagaucu agaagagcug ugacgcuccc cucccauucc acuaggaagc      420

ugcaaacgcg gucgcaaacc ugguuggaau caagagaaua cacaaagcac uugauuagag      480

ucgaaaauug gauauucagg aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc      540

uuuugggaag cucaacgagc caaaaaguca uauacuuggu caugauacug cugauugccc      600

cggcauacag caucaggugc auaggaguca gcaauaggga cuuuguggaa gguaugucag      660

gugggacuug gguugauauu gucuuggaac auggagguug ugucaccgua auggcacagg      720

acaaaccgac ugucgacaua gagcugguua caacaacagu cagcaacaug gcggagguaa      780

gauccuacug cuaugaggca ucaauaucag acauggcuuc ggacagccgc ugcccaacac      840

aaggugaagc cuaccuugac aagcaaucag acacucaaua ugucugcaaa agaacguuag      900

uggacagagg cuggggaaau ggauguggac uuuuuggcaa agggagucug gugacaugcg      960

cuaaguuugc augcuccaag aaaaugaccg ggaagagcau ccagccagag aaucuggagu     1020

accggauaau gcugucaguu cauggcuccc agcacagugg gaugaucguu aaugacacag     1080

gacaugaaac ugaugagaau agagcgaagg uugagauaac gcccaauuca ccaagagccg     1140

aagccacccu gggggguuuu ggaagccuag gacuugauug ugaaccgagg acaggccuug     1200

acuuuucaga uuuguauuac uugacuauga auaacaagca cugguugguu cacaaggagu     1260

gguuccacga cauuccauua ccuuggcacg cuggggcaga caccggaacu ccacacugga     1320

acaacaaaga agcacuggua gaguucaagg acgcacaugc caaaaggcaa acugucgugg     1380

uucuagggag ucaagaagga gcaguucaca cggcccuugc uggagcucug gaggcugaga     1440

uggauggugc aaagggaagg cuguccucug gccacuugaa augucgccug aaaauggaua     1500

aacuuagauu gaagggcgug ucauacuccu uguguaccgc agcguucaca uucaccaaga     1560

ucccggcuga aacacugcac gggacaguca caguggaggu acaguacgca gggacagaug     1620

gaccuugcaa gguuccagcu cagauggcgg uggacaugca aacucugacc ccaguuggga     1680

gguugauaac cgcuaacccc guaaucacug aaagcacuga gaacucuaag augaugcugg     1740

aacuugaucc accauuuggg gacucuuaca uugucauagg agucggggag aagaagauca     1800

cccaccacug gcacaggagu ggcagcacca uuggaaaagc auuugaagcc acugugagag     1860

gugccaagag aauggcaguc uugggagaca cagccuggga cuuuggauca guuggaggcg     1920

cucucaacuc auugggcaag ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu     1980

uuggaggaau guccugguuc ucacaaauuc ucauuggaac guugcugaug ugguuggguc     2040

ugaacacaaa gaauggaucu auuucccuua ugugcuuggc cuuaggggga guguugaucu     2100

ucuuauccac agccgucucu gcuugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  5322
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5322
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaua       60

ccagugucgg aauuguuggc cuccugcuga ccacagcuau ggcagcggag gucacuagac      120

gugggagugc auacuauaug uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc      180

caaccacauu ggggaugaau aaguguuaua uacagaucau ggaucuugga cacaugugug      240

augccaccau gagcuaugaa ugcccuaugc uggaugaggg gguggaacca gaugacgucg      300

auuguuggug caacacgacg ucaacuuggg uuguguacgg aaccugccau cacaaaaaag      360

gugaagcacg gagaucuaga agagcuguga cgcuccccuc ccauuccacu aggaagcugc      420

aaacgcgguc gcaaaccugg uuggaaucaa gagaauacac aaagcacuug auuagagucg      480

aaaauuggau auucaggaac ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu      540

ugggaagcuc aacgagccaa aaagucauau acuuggucau gauacugcug auugccccgg      600

cauacagcau caggugcaua ggagucagca auagggacuu uguggaaggu augucaggug      660

ggacuugggu ugauauuguc uuggaacaug gagguugugu caccguaaug gcacaggaca      720

aaccgacugu cgacauagag cugguuacaa caacagucag caacauggcg gagguaagau      780

ccuacugcua ugaggcauca auaucagaca uggcuucgga cagccgcugc ccaacacaag      840

gugaagccua ccuugacaag caaucagaca cucaauaugu cugcaaaaga acguuagugg      900

acagaggcug gggaaaugga uguggacuuu uuggcaaagg gagucuggug acaugcgcua      960

aguuugcaug cuccaagaaa augaccggga agagcaucca gccagagaau cuggaguacc     1020

ggauaaugcu gucaguucau ggcucccagc acagugggau gaucguuaau gacacaggac     1080

augaaacuga ugagaauaga gcgaagguug agauaacgcc caauucacca agagccgaag     1140

ccacccuggg ggguuuugga agccuaggac uugauuguga accgaggaca ggccuugacu     1200

uuucagauuu guauuacuug acuaugaaua acaagcacug guugguucac aaggaguggu     1260

uccacgacau uccauuaccu uggcacgcug gggcagacac cggaacucca cacuggaaca     1320

acaaagaagc acugguagag uucaaggacg cacaugccaa aaggcaaacu gucgugguuc     1380

uagggaguca agaaggagca guucacacgg cccuugcugg agcucuggag gcugagaugg     1440

auggugcaaa gggaaggcug uccucuggcc acuugaaaug ucgccugaaa auggauaaac     1500

uuagauugaa gggcguguca uacuccuugu guaccgcagc guucacauuc accaagaucc     1560

cggcugaaac acugcacggg acagucacag uggagguaca guacgcaggg acagauggac     1620

cuugcaaggu uccagcucag auggcggugg acaugcaaac ucugacccca guugggaggu     1680

ugauaaccgc uaaccccgua aucacugaaa gcacugagaa cucuaagaug augcuggaac     1740

uugauccacc auuuggggac ucuuacauug ucauaggagu cggggagaag aagaucaccc     1800

accacuggca caggaguggc agcaccauug gaaaagcauu ugaagccacu gugagaggug     1860

ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu ggaggcgcuc     1920

ucaacucauu gggcaagggc auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug     1980

gaggaauguc cugguucuca caaauucuca uuggaacguu gcugaugugg uugggucuga     2040

acacaaagaa uggaucuauu ucccuuaugu gcuuggccuu agggggagug uugaucuucu     2100

uauccacagc cgucucugcu ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5323
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 wild
       type

<400>  5323
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5324
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5324
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggccguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5325
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5325
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gcgcagauac cagugucgga auuguuggcc uccugcugac cacagcuaug gcagcggccg      120

ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu ggggaggcca      180

uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug gaucuuggac      240

acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg guggaaccag      300

augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga accugccauc      360

acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc cauuccacua      420

ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca aagcacuuga      480

uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca gcugccaucg      540

cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug auacugcuga      600

uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu guggaaggua      660

ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc accguaaugg      720

cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc aacauggcgg      780

agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac agccgcugcc      840

caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc ugcaaaagaa      900

cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg agucugguga      960

caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag ccagagaauc     1020

uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug aucguuaaug     1080

acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc aauucaccaa     1140

gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa ccgaggacag     1200

gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg uugguucaca     1260

aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac     1320

acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa aggcaaacug     1380

ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga gcucuggagg     1440

cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa     1500

uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg uucacauuca     1560

ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag uacgcaggga     1620

cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu cugaccccag     1680

uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac ucuaagauga     1740

ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc ggggagaaga     1800

agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu gaagccacug     1860

ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu ggaucaguug     1920

gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca gcuuucaaau     1980

cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug cugauguggu     2040

ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua gggggagugu     2100

ugaucuucuu auccacagcc gucucugcuu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5326
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5326
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuguccuc aaggaaacgc cguucccaug auguucugac ugugcaauuc cuaauuuugg      120

gaaugcuguu gaugacgggu ggagugaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5327
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 wild type

<400>  5327
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuguccuc aaggaaacgc cguucccaug auguucugac ugugcaauuc cuaauuuugg      120

gaaugcuguu gaugacgggu gccgugaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5328
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 2 wild type

<400>  5328
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac ggggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5329
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 2 wild type

<400>  5329
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacacgug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aaucuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacgcaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5330
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 2 wild type

<400>  5330
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg     1140

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga agcuuaggac     1200

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca     1260

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uucaaggaug     1380

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg     1440

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc     1500

auuugaaaug ccgccuaaaa auggacaagc uuagauugaa gggcguguca uauuccuugu     1560

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1620

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1680

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1740

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1800

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1860

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1920

ccugggacuu cggaucaguc gggggugugu ucaacucacu ggguaagggc auucaccaga     1980

uuuuuggagc agccuucaaa ucacuguuug gaggaauguc cugguucuca cagauccuca     2040

uaggcacgcu gcuagugugg uuagguuuga acacaaagaa uggaucuauc ucccucacau     2100

gcuuggcccu ggggggagug augaucuucc ucuccacggc uguuucugcu gacguggggu     2160

gcucagugga cuucucaaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5331
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 2 wild type

<400>  5331
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguuggucc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5332
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 2 wild type

<400>  5332
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca guugacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augauuguca augauacagg auaugaaacu gacgaaaaua     1140

gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug ggaggcuuug     1200

gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau cuguauuacc     1260

ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac aucccauugc     1320

cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag gcauugguag     1380

aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc caggaaggag     1440

ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca aagggaaagc     1500

uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug aagggcgugu     1560

cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa acacugcaug     1620

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag aucccagucc     1680

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaaccccg     1740

ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg cauaggagug     1860

guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga auggcagucc     1920

ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca cuggguaagg     1980

gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag aauggaucua     2100

ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg gcuguuucug     2160

cugacguggg gugcucagug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5333
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 2 wild type

<400>  5333
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aggaagagac guggcgcaga caccagcauc ggaaucguug      120

gccuccuguu gacuacagcc auggcagcag agaucacuag acgugggagu gcauacuaca      180

uguacuugga uaggagcgau gcagggaagg ccauuucuuu cgcuaccaca uuggggguga      240

acaaaugcca ugugcagauc auggaccucg ggcacaugug ugacgccacc augagcuaug      300

aaugcccuau gcuggacgag gggguggaac cagaugacgu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucauaaaaa aggugaagca cggcgaucua      420

gaagagccgu cacgcuccca ucucacucca caaggaaauu gcaaacgcgg ucgcagacuu      480

ggcuagaauc aagagaauac acaaagcacc ugaucaaggu ugaaaauugg auauucagga      540

acccugguuu uacgcuagug gcugucgcca ucgccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uaggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugacguug      720

uccuggaaca uggaggcugc gucaccguga uggcacagga caagccaaca guugacauag      780

agcuggucac aacaacgguu aguaacaugg ccgaggugag auccuauugu uacgaggcau      840

caauaucgga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau guuugcaaaa gaacauuggu ggacagaggu uggggaaaug      960

gguguggacu cuuuggcaaa gggaguuugg ugacaugugc uaaguucacg ugcuccaaga     1020

agaugacugg gaagagcauu cagccggaga accuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcacaguggg augauuguua augaugaaaa cagagcgaag gucgagguua     1140

cgcccaauuc accaagagca gaagcaaccc ugggaggcuu uggaagcuua ggacuugauu     1200

gugaaccaag gacaggccuu gacuuuucag aucuguauua ccuaaccaug aauaacaagc     1260

auugguuggu gcacaaagag ugguuucaug acaucccauu gcccuggcau gcuggggcag     1320

acacuggaac uccacauugg aacaacaagg aggcauuagu ggaauucaag gacgcccacg     1380

ccaagaggca aaccgucgug guuuugggga gccaggaagg agccguccac acggcucuug     1440

cuggagcucu agaggcugag auggauggug caaagggaag gcuauucucu ggccacuuga     1500

aaugucgcuu aaaaauggac aagcuuagau ugaagggcgu gucauauucc uugugcaccg     1560

cggcauucac auucaccaag gucccggcug aaacacuaca uggaacaguc acaguggagg     1620

ugcaguaugc agggacagau ggacccugca aggucccagc ccagauggcg guggacaugc     1680

agaccuugac cccagucgga aggcugauaa ccgccaaccc cgugauuacu gaaagcacug     1740

agaauucaaa gaugauguug gagcucgacc caccauuugg ggauucuuac auugucauag     1800

gaguugggga uaagaaaauc acccaucacu ggcauaggag uggcagcacc auuggaaaag     1860

cauuugaagc cacugugaga ggcgcuaaga gaauggcagu ccugggggac acagcuuggg     1920

acuuuggauc agucggaggu guguuuaacu cauugggcaa gggcauucau cagauuuuug     1980

gagcagcuuu caaaucacug uuuggaggaa uguccugguu cucacagauc cucauaggca     2040

cucugcuggu gugguuaggu cugaacacaa agaauggguc uaucucccuc acaugcuuag     2100

cccugggggg agugaugauc uuccucucca cggcuguuuc ugcugacgug gggugcucgg     2160

uggacuucuc aaagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5334
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 2 wild type

<400>  5334
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucgcaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca guugacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augauuguca augauacagg auaugaaacu gacgaaaaua     1140

gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug ggaggcuuug     1200

gaagcuuagg acuugacugu gaaccaagga caggucuuga cuuuucagau cuguauuacc     1260

ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac aucccauugc     1320

cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag gcauugguag     1380

aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc caggaaggag     1440

ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca aagggaaagc     1500

uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug aagggcgugu     1560

cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa acacugcaug     1620

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag aucccagucc     1680

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaaccccg     1740

ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg cauaggagug     1860

guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga auggcagucc     1920

ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca cuggguaagg     1980

gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag aauggaucua     2100

ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg gcuguuucug     2160

cugacguggg gugcucagug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5335
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 2 wild type

<400>  5335
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca guugacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augacuguca augauauagg auaugaaacu gacgaaaaua     1140

gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug ggaggcuuug     1200

gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau cuguauuacc     1260

ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac aucccauugc     1320

cuuggcaugc uggggcagac acuggaacuc cacacuggaa caacaaagag gcauugguag     1380

aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc caggaaggag     1440

ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca aagggaaagc     1500

uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug aagggcgugu     1560

cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa acacugcaug     1620

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag aucccagucc     1680

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaaccccg     1740

ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg cauaggagug     1860

guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga auggcagucc     1920

ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca cuggguaagg     1980

gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag aauggaucua     2100

ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg gcuguuucug     2160

cugacguggg gugcucagug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5336
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 2 wild type

<400>  5336
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ccggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augauuggau augaaacuga cgaaaauaga gcgaaagucg     1140

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga agcuuaggac     1200

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca     1260

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug     1320

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uucaaggaug     1380

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg     1440

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaagcug uucucuggcc     1500

auuugaaaug ccgccuaaaa auggacaagc uuagauugaa gggcguguca uauuccuugu     1560

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1620

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1680

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1740

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1800

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1860

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1920

ccugggacuu cggaucaguc gggggugugu ucaacucacu ggguaagggc auucaccaga     1980

uuuuuggagc agccuucaaa ucacuguuug gaggaauguc cugguucuca cagauccuca     2040

uaggcacgcu gcuagugugg uuagguuuga acacaaagaa uggaucuauc ucccucacau     2100

gcuuggcccu ggggggagug augaucuucc ucuccacggc uguuucugcu gacguggggu     2160

gcucagugga cuucucaaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5337
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 2 wild type

<400>  5337
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaggg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5338
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 2 wild type

<400>  5338
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga ugcuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5339
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 2 wild type

<400>  5339
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuagaaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggccu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5340
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 2 wild type

<400>  5340
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccuacu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg uacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5341
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 2 wild type

<400>  5341
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagauaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacuggugg     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcugcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5342
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 2 wild type

<400>  5342
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guucuagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5343
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 2 wild type

<400>  5343
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuac gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc agcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcuggucac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcaguaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5344
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 2 wild type

<400>  5344
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucuag aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacugaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acuccuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gucgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5345
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 2 wild type

<400>  5345
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucagcuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ccgcaaaccu      480

gguuggaauc aagagaauac acaaagcauu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacaagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5346
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 2 wild type

<400>  5346
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagu ucaacgagca      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauugggacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5347
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 2 wild type

<400>  5347
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgcgcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucuag aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacugaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acuccuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5348
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 2 wild type

<400>  5348
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaagcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacu augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caauaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacacgcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5349
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 2 wild type

<400>  5349
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaauguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaacuu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacuccaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaggaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5350
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 2 wild type

<400>  5350
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga aacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagccgu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccugguuu cgcuuuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug uugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5351
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 2 wild type

<400>  5351
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca ccaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcauu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacaagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc cucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucu gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5352
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 2 wild type

<400>  5352
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaauguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaacugg auguucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag gucccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5353
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 2 wild type

<400>  5353
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

agugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auacucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auauuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acucagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga aucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5354
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 2 wild type

<400>  5354
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuuc aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcaa      840

caauaucaga cauagcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugccguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5355
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 2 wild type

<400>  5355
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc caggaaggag aagaagagac gaggcgcaga uacuaguguc ggaaucguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggggguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagcuuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggacu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5356
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 2 wild type

<400>  5356
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca ccaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucu gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaagu ugagauaacg cccaauucac cgagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaguua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5357
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 2 wild type

<400>  5357
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga aacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga uagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagccgu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagaau cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaua      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagccc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5358
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 2 wild type

<400>  5358
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcgcuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauauagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccaccuug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcauugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggugc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu caucggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5359
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 2 wild type

<400>  5359
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugua aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaaa ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

uaggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuuccuuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5360
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 2 wild type

<400>  5360
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaca      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgccuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaagc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccucau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5361
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 2 wild type

<400>  5361
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaca      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagcuuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga agcacaugcu aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccucau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5362
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 2 wild type

<400>  5362
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucaccag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuggu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcau ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag gggcagaugg acccugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5363
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 2 wild type

<400>  5363
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc ugggaaggag aagaagagac gaggcgcaga uacuaauguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaacuu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacgu ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag gcugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacuccaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaggaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5364
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 2 wild type

<400>  5364
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga aacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaaagau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagccgu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccugguuu cgcuuuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau guuugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug uugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cauaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5365
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 2 wild type

<400>  5365
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcag aggucacuag acgugggagu gcauacuaca      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga caugguuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuucucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccucau gugcuuggcc uuagggggag ugcugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5366
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 2 wild type

<400>  5366
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaauguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacauga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugaccugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguccacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

guagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aacggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5367
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 2 wild type

<400>  5367
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc caggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug cgaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaauacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccgcccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaacucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5368
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 2 wild type

<400>  5368
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga aacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagccgu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccugguuu cgcuuuagca gcagcugcca ucgcguggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccu ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug uugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagccc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5369
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 2 wild type

<400>  5369
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaca      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca uagcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggaaucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaguau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaauccuagg acuggauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccucau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5370
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 2 wild type

<400>  5370
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aagucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacacgug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca uuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcaug     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacgcaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5371
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 2 wild type

<400>  5371
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaca      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu cccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cagagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaaguuau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggaggcugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacccaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuggauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagauac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccucau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5372
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 2 wild type

<400>  5372
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gccggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaagcgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauauagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu guugacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagcuugg ugacaugcgc uaaguuugca ugcuccaaga     1020

agaugaccgg gaagagcauc cagccagaga auuuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaca     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaagagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggugc ucuuaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5373
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 2 wild type

<400>  5373
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca cuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguagaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaguc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga uacccaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggaaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucagucc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguuggucc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggacaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg acccugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcaguaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggccu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5374
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 2 wild type

<400>  5374
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcacaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca cuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguagaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucca      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag uaauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa caacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacac uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aggcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgu ucuuaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaauacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucuccg     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5375
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 2 wild type

<400>  5375
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca uuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccu ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccgcaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau guuugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augcucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguuggcuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagcc accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acaguggacg     1620

ggacagucac aguggaggga caguacggag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcag acucugaccc caguugggag guugauaacc gcuaaccccg     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuauugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauugggacg uugcugaugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucag     2160

gugguguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5376
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 2 wild type

<400>  5376
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcacaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcc auggcagcgg aagucacuag acgugggagu gcauacuaua      180

uguacuugga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca cuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguagaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc accacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuggca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucuccaagau cccggcugaa acacugcacg     1620

ggacagucac agaggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccgg     1740

uaaucacuga aagcacagag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaauacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5377
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 2 wild type

<400>  5377
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc caggaaggag aagaagagac gaggcacaga cacuaguguc ggaauuguug      120

gccuccugcu gaccacagcc auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca cuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguagaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc accacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccacucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu cuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcucc ggcauauagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggcgaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc caaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccgaauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggagguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aauucaagga ugcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagagggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacucccu guguaccgca gcguucacau ucacuaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaauacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5378
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 2 wild type

<400>  5378
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcacaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcc auggcagugg aggucacuag acgugggaau gcauacuaua      180

uguacuugga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca auggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguagaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc accacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauac acaaagcacc ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu guuaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugcu ugcucuaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augauacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugcc aaaaggcaga cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucacuaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1740

uaaucacuga aagcacugag aacuccaaga ugaugcugga acuggaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaaa agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uugggggugc ucucaacuca cugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcuggugu gguugggucu gaauacaaag aauggaucua     2100

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5379
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 2 wild type

<400>  5379
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggaa aagaagagac gaggcacaga cacuaguguc ggaauuguug      120

gccuccugcu gaccacagcc auggcagcgg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca cuggggauga      240

auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau gcuggaugag gggguagaac cagaugacgu cgauuguugg ugcaacacga      360

cgucaacuug gguuguguac ggaaccugcc accacaaaaa aggugaagcg cggagaucua      420

gaagagcugu gacgcucccc ucccacucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuggca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauauagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugaugucg      720

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cagagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc caaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccgaauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggagguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1380

aguucaagga ugcacaugcc aaaaggcaaa cugucguggu ccuagggagu caagagggag     1440

caguucacac ggcucuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagacug aagggcgugu     1560

cauacucccu guguaccgca gcguucacau ucacuaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1980

guauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaauacaaag aauggaucua     2100

uuucccuugu gugcuuggcc uuagggggag uguugaucuu cuuguccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5380
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 2 wild type

<400>  5380
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcacaga uacuaguguc ggaauuguug      120

gccuccugcu gaccacagcc auggcagugg aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca cuggggauga      240

acaaguguua cauacagauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau guuggaugag gggguagaac cagaugacgu cgauuguugg ugcaacacga      360

caucaacuug gguuguguac ggaaccugcc accacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauau acaaagcacc ugauuagagu cgaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcugcca ucgccuggcu uuugggaagu ucaacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu guuaccguaa uggcacagga caaaccggcu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuauugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcagucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagcuga agccacccug ggggguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcaugc uggggcagac accggaacuc cacauuggaa caacaaagaa gcauugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg acccugcaag guuccagcuc     1680

agauggcggu ggacaugcaa acucugaccc caguugggag gcugauaacc gcuaacccug     1740

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaucacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggau uuuggaucag uuggaggugc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcuggugu gguugggucu gaauacaaag aauggaucua     2100

uuucccuuac gugcuuggcc uuagggggag uguugaucuu uuuauccaca gccgucucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5381
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 2 wild type

<400>  5381
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac gaggcgcaga uacuagcguc ggaauuguug      120

gccuccuccu gaccacagcc auggcaguag aggucacuag acgugggagu gcauacuaua      180

uguacuugga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca cuggggauga      240

auaaguguua cauacaaauc auggaucuug gacacaugug ugaugccacc augagcuaug      300

aaugcccuau guuggaugag gggguagaac cagaugacgu cgauugcugg ugcaacacga      360

caucaacuug gguuguguau ggaaccugcc accacaaaaa aggugaagca cggagaucua      420

gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauac acaaagcacc ugauuagagu ugaaaauugg auauucagga      540

acccuggcuu cgcguuagca gcagcuguca ucgcuuggcu uuugggaagu ucaacgagcc      600

aaaaagucau auaucugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      720

ucuuggaaca uggagguugu guuaccguaa uggcacagga caaaccgacu gucgacauag      780

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      840

caauaucgga uauggcuucg gacagccgcu gcccaacaca aggugaggcc uaccuugaca      900

agcagucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      960

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga     1020

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug     1200

ggagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacc     1260

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1320

cuuggcaugc uggggcagac acuggaacuc cacauuggaa caacaaagaa gcacugguag     1380

aguucaagga cgcacaugca aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggagcc aagggaaggc     1500

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1560

cauacuccuu gugcacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1620

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1680

agauggcggu ggauaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1740

uaaucacuga aagcaccgag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1800

acucuuacau ugucauagga gucggggaga agaagaucac ccaucacugg cacaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag uugggggugc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu cggaggaaug uccugguucu     2040

cacaaauucu cauuggaacg uugcuggugu gguugggucu gaauacaaag aauggaucua     2100

uuucccuuac gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccguuucug     2160

cugauguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5382
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 2 wild type

<400>  5382
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac guggcgcaga caccaguguc ggaauuguug      120

gccuccugcu gaccacagcc auggcagugg aggucaccag acgugggagu gcauacuaua      180

uguacuuaga cagaagcgau gcuggggagg ccauaucuuu uccaaccaca cuggggguga      240

auaaguguua cauacagauc auggaucuug gacacaugug ugaugccaca augagcuaug      300

aaugcccuau guuggaugag gggguagaac cagaugacgu cgauugcugg ugcaacacga      360

caucgacuug gguuguguac ggaaccugcc aucacaaaaa aggugaggca cggagaucua      420

gaagagcugu gacgcucccc ucucauucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauac acaaagcacu ugaucagagu cgaaaauugg auauucagga      540

acccuggcuu ugcguuggca gcagcugcca uugcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugu ugauugcccc ggcauacagu aucaggugca      660

uaggagucag caauagggau uuuguggaag guaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggagguugu guuaccguaa uggcacagga caagccaacu guugauauag      780

aguuggucac aacaacgguu agcaacaugg cggagguaag auccuacugc uacgaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcagucaga cacucaauau guuugcaaaa gaacguuagu ggacagaggu uggggaaaug      960

gauguggacu cuuuggcaaa gggagccugg ugacaugcgc caaguuugca ugcuccaaga     1020

aaaugacugg gaagagcauc cagccagaga accuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augauuguua augacacagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggagguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcau ugguuggugc acaaggagug guuccaugac auuccacuac     1320

cuuggcaugc uggggcagac accggaacuc cacauuggaa caacaaagaa gcauugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

ccguucacac ggcucuugcu ggagcccugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgcuuga aaauggacaa acuuagauug aagggcgugu     1560

cauacuccuu auguaccgcg gcguucacau ucaccaagau cccggcugaa acgcugcaug     1620

ggacagucac aguggaggua caguaugcag ggacagaugg acccugcaag guuccagcuc     1680

agauggcggu ggauaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1740

ugaucacuga aagcacugag aauucaaaga ugauguugga acuugaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaua agaagaucac ccaccacugg uacaggagug     1860

gcagcaccau cggaaaagca uuugaagcca cugugagagg cgccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag ucggaggugc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauaggaacg uugcuggugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuac gugcuuggcc uuagggggag uguugaucuu ccuaucuaca gccgucucug     2160

cugauguggg guguucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5383
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 2 wild type

<400>  5383
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aagaagagac guggcgcaga caccaguguc ggaauuguug      120

gccuccugcu gaccacagcc auggcagugg aggucaccag acgugggagu gcauacuaua      180

uguacuuaga cagaagcgau gcugggaagg ccauaucuuu uccaaccaca cuggggguga      240

auaaguguua cauacagauc auggaucuug gacacaugug ugaugccaca augagcuaug      300

aaugcccuau guuggaugag gggguagaac cagaugacgu cgauugcugg ugcaacacga      360

caucgacuug gguuguguac ggaaccugcc aucacaaaaa aggugaggca cggagaucua      420

gaagagcugu gacgcucccc ucucauucca cuaggaagcu gcaaacgcgg ucgcagaccu      480

gguuggaauc aagagaauac acaaagcacu ugaucagagu cgaaaauugg auauucagga      540

acccuggcuu ugcguuggca gcagcugcca uugcuuggcu uuugggaagc ucaacgagcc      600

aaaaagucau auacuugguc augauacugu ugauugcccc ggcauacagu aucaggugca      660

uaggagucag caauagggau uuuguggaag guaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggagguugu guuaccguaa uggcacagga caagccaacu guugauauag      780

aguuggucac aacaacgguu agcaacaugg cggagguaag auccuacugc uacgaggcau      840

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      900

agcagucaga cacucaauau guuugcaaaa gaacguuagu ggacagaggu uggggaaaug      960

gauguggacu cuuuggcaaa gggagccugg ugacaugcgc caaguuugca ugcuccaaga     1020

aaaugacugg gaagagcauc cagccagaga accuggagua ccggauaaug cugucaguuc     1080

auggcuccca gcacaguggg augauuguua augacauagg acaugaaacu gaugagaaua     1140

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggagguuuug     1200

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu     1260

ugacuaugaa uaacaagcau ugguuggugc acaaggagug guuccaugac auuccacuac     1320

cuuggcaugc uggggcagac accggaacuc cacauuggaa caacaaagaa gcauugguag     1380

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1440

ccguucacac ggcucuugcu ggagcccugg aggcugagau ggauggugca aagggaaggc     1500

uguccucugg ccacuugaaa ugucgcuuga aaauggacaa acuuagauug aagggcgugu     1560

cauacuccuu auguaccgcg gcguucacau ucaccaagau cccggcugaa acgcugcaug     1620

ggacagucac aguggaggua caguaugcag ggacagaugg acccugcaag guuccagcuc     1680

agauggcggu ggauaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1740

ugaucacuga aagcacugag aauucaaaga ugauguugga acuugaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaua agaagaucac ccaccacugg cacaggagug     1860

gcagcaccau cggaaaagca uuugaagcca cugugagagg cgccaagaga auggcagucu     1920

ugggagacac agccugggac uuuggaucag ucggaggugc ucucaacuca uugggcaagg     1980

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     2040

cacaaauccu cauaggaacg uugcuggugu gguugggucu gaacacaaag aauggaucua     2100

uuucccuuac gugcuuggcc uuagggggag uguugaucuu ccuaucuaca gccgucucug     2160

cugauguggg guguucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5384
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 2 wild type

<400>  5384
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aggaagagac guggcgcaga caccagcauc ggaaucguug      120

gccuccuguu gacuacagcc auggcagcag agaucacuag acgugggagu gcauacuaca      180

uguacuugga uaggagcgau gcagggaagg ccauuucuuu cgcuaccaca uuggggguga      240

acaaaugcca ugugcagauc auggaccucg ggcacaugug ugacgccacc augagcuaug      300

aaugcccuau gcuggacgag gggguggaac cagaugacgu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucauaaaaa aggugaagca cggcgaucua      420

gaagagccgu cacgcuccca ucucacucca caaggaaauu gcaaacgcgg ucgcagacuu      480

ggcuagaauc aagagaauac acaaagcacc ugaucaaggu ugaaaauugg auauucagga      540

acccugguuu uacgcuagug gcugucgcca ucgccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uaggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugacguug      720

uccuggaaca uggaggcugc gucaccguga uggcacagga caagccaaca guugacauag      780

agcuggucac aacaacgguu aguaacaugg ccgaggugag auccuauugu uacgaggcau      840

caauaucgga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau guuugcaaaa gaacauuggu ggacagaggu uggggaaaug      960

gguguggacu cuuuggcaaa gggaguuugg ugacaugugc uaaguucacg ugcuccaaga     1020

agaugacugg gaagagcauu cagccggaga accuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcacaguggg augauuguua augacacagg acaugaaacu gaugaaaaca     1140

gagcgaaggu cgagguuacg cccaauucac caagagcaga agcaacccug ggaggcuuug     1200

gaagcuuagg acuugauugu gaaccaagga caggccuuga cuuuucagau cuguauuacc     1260

uaaccaugaa uaacaagcau ugguuggugc acaaagagug guuucaugac aucccauugc     1320

ccuggcaugc uggggcagac acuggaacuc cacauuggaa caacaaggag gcauuagugg     1380

aauucaagga cgcccacgcc aagaggcaaa ccgucguggu uuuggggagc caggaaggag     1440

ccguccacac ggcucuugcu ggagcucuag aggcugagau ggauggugca aagggaaggc     1500

uauucucugg ccacuugaaa ugucgcuuaa aaauggacaa gcuuagauug aagggcgugu     1560

cauauuccuu gugcaccgcg gcauucacau ucaccaaggu cccggcugaa acacuacaug     1620

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag gucccagccc     1680

agauggcggu ggacaugcag accuugaccc cagucggaag gcugauaacc gccaaccccg     1740

ugauuacuga aagcacugag aauucaaaga ugauguugga gcucgaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaua agaaaaucac ccaucacugg cauaggagug     1860

gcagcaccau uggaaaagca uuugaagcca cugugagagg cgcuaagaga auggcagucc     1920

ugggggacac agcuugggac uuuggaucag ucggaggugu guuuaacuca uugggcaagg     1980

gcauucauca gauuuuugga gcagcuuuca aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcacu cugcuggugu gguuaggucu gaacacaaag aaugggucua     2100

ucucccucac augcuuagcc cuggggggag ugaugaucuu ccucuccacg gcuguuucug     2160

cugacguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5385
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 2 wild type

<400>  5385
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aggaagagac guggugcaga caccagcauc ggaaucauug      120

gucuccugcu gacuacagcc auggcagcgg agauuaccag acguggaagu gcauacuaca      180

uguacuugga caggagcgau gcugggaagg ccaucuccuu ugcuaccaac uugggaguua      240

acaaaugcca uguacagauc auggaucucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau guuggacgag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaagca cggcgaucua      420

gaagagcugu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacc ugaucaaggu ugaaaacugg auauuuagga      540

accccggguu ugcgcucgca gcuguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguu augauacugc ugauugcccc ggcauacagc aucaggugca      660

uuggagucag caauagagac uucguggaag gcaugucagg ugggaccugg guugaugucg      720

ucuuggaaca uggagguugc gucaccguua uggcacagga caagccaaca guugacauag      780

aguuggucac gacaacgguu aguaacaugg ccgagguaag auccuacugu uaugaggcau      840

caauaucaga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaua      900

agcaaucaga cacccaauau gugugcaaaa gaacauuggu ggacagaggu uggggaaaug      960

gguguggacu uuuuggcaaa gggagccugg ugacaugugc caaguucacg uguuccaaga     1020

agaugacugg gaagagcauu cagccggaga aucuggagua ucgaauaaug cuaucagugc     1080

auggcuccca gcacagcggg augauuguua augacauagg acaugaaacu gacgaaaaca     1140

gagcgaaagu cgaggucacg cccaauucac caagagcaga agcaaccuug ggaggcuuug     1200

gaagcuuagg acuugauugu gaaccaagga caggccugga cuuuucagau uuguacuacu     1260

ugaccaugaa caacaagcac ugguuggugc acaaagagug guuucaugac aucccauugc     1320

cuuggcaugc uggggcagau accggaacuc cacacuggaa caacaaggag gcauugguag     1380

aguucaaaga ugcccacgcc aagaggcaaa ccgucguugu ucuagggagc caggaaggag     1440

ccguucacac ggcucuugcu ggagcucugg aggcugagau ggauggugca aaaggaaggc     1500

uguucuccgg ccauuugaaa ugccgcuuaa aaauggacaa gcuuagauug aagggcgugu     1560

cauauuccuu gugcaccgca gcguucacau uuaccaaggu cccagcugaa acauugcaug     1620

gaacaguuac aguggaggug caguaugcag ggacagaugg acccugcaag gucccagccc     1680

agauggcggu ggacaugcag acccugaccc cagucgggag gcugauaacc gccaacccug     1740

ugauuacuga aagcacugag aauucaaaga ugauguugga gcucgaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaca agaaaaucac ccaucacugg cauaggagug     1860

guagcaccau cggaaaagca uuugaggcca cugugagagg ugccaagaga auggcaguuc     1920

ugggggauac agccugggac uucggaucag ucgggggcgu guucaacuca uugggcaagg     1980

guguucacca gauuuuugga gcagcuuuca aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcacg cugcuggugu gguuggguuu gaacacaaag aauggaucca     2100

ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg gcugucucug     2160

cugacguggg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5386
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 2 wild type

<400>  5386
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcgaucua      420

gaagagccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca guugacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaaug      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguucacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc     1080

auggcuccca gcauagcggg augauuguca augauauagg acaugaaacu gacgaaaaca     1140

gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug ggaggcuuug     1200

gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau cuguauuacc     1260

ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac aucccauugc     1320

cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag gcauugguag     1380

aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc caggaaggag     1440

ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca aagggaaggc     1500

uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug aagggcgugu     1560

cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa acacugcaug     1620

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag gucccagccc     1680

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaaccccg     1740

ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg cauaggagug     1860

guagcaccau cggaaaggca uuugaagcca cugugagagg cgccaagaga auggcagucc     1920

ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca cuggguaagg     1980

gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag aauggaucua     2100

ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg gcuguuucug     2160

cugacguggg gugcucagug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5387
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 2 wild type

<400>  5387
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aggaagagac guggugcaga caccagcauc ggaaucauug      120

gucuccugcu gacuacagcc auggcagcag agauuaccag acguggaagu gcauacuaca      180

uguacuugga caggagcgau gcugggaagg ccaucuccuu ugcuaccaau uugggaguua      240

acaaaugcca uguacagauc auggaucucg ggcacaugug ugacgccacc augaguuaug      300

aguguccuau guuggacgag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaagca cggcgaucua      420

gaagagcugu gacgcucccu ucucacucua caaggaagcu gcaaacgcgg ucgcagacuu      480

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      540

accccggguu ugcgcucgca gcuguugcca uugccuggcu uuugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      660

uuggagucag caauagagac uucguggaag gcaugucagg ugggaccugg guugaugucg      720

ucuuggaaca uggagguugc gucaccguaa uggcacagga caagccaaca guugacauag      780

aguuggucac gacaacgguc aguaacaugg ccgagguaag auccuacugu uaugaggcau      840

caauaucgga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau guaugcaaaa gaacauuggu ggacagaggu uggggaaaug      960

gguguggacu uuuuggcaaa gggagccugg ugacaugugc caaguucacg uguuccaaga     1020

agaugaccgg gaagagcauu cagccggaga aucuggagua ucgaauaaug cuaucagugc     1080

auggcuccca gcacagcggg augauuguua augacgaaaa uagggcgaaa gucgagguca     1140

cgcccaauuc accaagagca gaagcaaccu ugggaggcuu uggaagcuua ggacuugauu     1200

gugaaccaag gacaggccug gacuuuucag auuuguacua cuugaccaug aacaauaagc     1260

acugguuagu gcacaaagag ugguuucaug acaucccauu gcccuggcau gcuggggcag     1320

acaccggaac uccacauugg aacaacaagg aggcauuggu agaguucaaa gaugcccacg     1380

cuaagaggca aaccgucguu guucuaggga gccaggaagg agccguucac acggcucuug     1440

cuggagcucu ggaggcugag auggauggug caaaaggaag guuguucucc ggccauuuga     1500

aaugccgcuu aaaaauggac aagcuuagau ugaagggcgu gucauauucc uugugcaccg     1560

cagcguucac auucaccaag gucccagcug aaacauugca uggaacaguu acaguggagg     1620

ugcaguaugc agggacagau ggacccugca aggucccagc ucagauggcg guggacaugc     1680

agacccugac cccagucgga aggcugauaa ccgccaaccc cgugauuacu gaaagcacug     1740

agaauucaaa gaugauguug gagcucgacc caccauuugg ggauucuuac auugucauag     1800

gaguugggga caagaaaauu acccaccacu ggcauaggag ugguagcacc aucggaaaag     1860

cauuugaggc cacugugaga ggugccaaga gaauggcagu ucugggggac acagccuggg     1920

acuuuggauc agucgggggc guguuuaacu cauugggcaa gggcauccac cagauuuuug     1980

gagcagccuu caaaucacug uuuggaggaa uguccugguu cucacagauc cucauaggca     2040

cgcugcuggu gugguugggu uugaacacaa agaauggauc caucucccuc acaugcuugg     2100

cccugggggg agugaugauc uuccucucca cggcugucuc ugcugacgug gggugcucgg     2160

uggacuucuc aaagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5388
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 2 wild type

<400>  5388
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuaucaaugc uaggaaggag aggaagagac guggcgcuga caccagcauc ggaaucgucg      120

gccuccugcu gaccacagcc auggcagccg agaucacuag acgugggagu gcauacuaca      180

uguacuugga caggagcgau gcugguaagg ccauuucuuu ugccaccaca uuggggguga      240

acaaaugcca uguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugccccau gcuagacgag ggaguggagc cagaugacgu cgauugcugg ugcaacacga      360

caucgacuug gguuguguac ggaaccuguc aucauaaaaa aggugaagca cgacgaucca      420

gaagagccgu gacgcuuccu ucucacucca cgaggaagcu gcaaacgcga ucgcagacuu      480

ggcuagaguc aagagaauac acaaagcacc ugaucaaggu ugaaaauugg auauucagga      540

accccggguu ugcgcuagug gcuguagcua uagccuggcu ccugggaagc ucgacgagcc      600

aaaaaguuau auacuugguc augauauugu ugauugcccc ggcauacagc auuaggugca      660

uaggaguuag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggggguugc gucaccguga uggcacagga caagccaaca guugauaucg      780

aguuggucac gacaacgguu agcaacaugg ccgagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccguu guccaacaca aggugaagcc uaccuugaca      900

agcagucaga cacucaauau gucugcaaga gaacauuggu ggauagaggu uggggaaaug      960

gguguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg ugcuccaaga     1020

aaaugacagg caagagcauc cagccggaga acuuggagua ccggauaaug cuaucagugc     1080

auggauccca gcacaguggg augauuguga augacacagg acaugaaacu gacgaaaaca     1140

gagcaaaagu cgaggucaca cccaauucac caagagcaga agcaaccuug ggagguuuug     1200

gaagcuuggg acuugacugu gaaccaagga caggccuuga cuucucagau cuguauuacu     1260

ugaccaugaa caauaagcau ugguuggugc acaaggagug guuucaugac aucccauuac     1320

cuuggcaugc uggugcagac acuggaacuc cacacuggaa caacaaagag gcauuggugg     1380

aguucaagga cgcccacgcc aagaggcaaa cuguuguggu ucuggggagc caagagggag     1440

cuguucauac ggcccucgcu ggagcuuugg aggcugagau ggauggugca aagggaaggc     1500

uauucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagguug aagggugugu     1560

cauauucccu guguaccgca gcguucacau uuaccaaggu cccagcugaa acauugcaug     1620

gaacagucac aguggaggug caguaugcag ggacagacgg acccugcaaa gucccagccc     1680

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaauccug     1740

ugaucacuga aaguacugag aauucaaaga ugauguugga gcucgaccca ccauuugggg     1800

auucuuacau ugucauagga gucggggaca agaaaaucac ccaucacugg caucggagug     1860

guagcaccau cggaaaggca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggggacac agccugggac uuuggaucag uugggggugu guuuaauuca uuggguaagg     1980

guauucacca gaucuuugga gcagcuuuca aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcaca cuguuggugu gguuagguuu gaacacaaag aauggaucua     2100

ucucccucac augcuuagcc cuggggggag ugaugaucuu ccuuuccacg gcuguuucug     2160

cugauguugg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5389
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 2 wild type

<400>  5389
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuaucaaugc uaggaaggag aggaagagac guggcgcuga caccagcauc ggaaucgucg      120

gccuccugcu gaccacagcc auggcagccg agaucaccag acgugggagu gcauacuaca      180

uguacuugga caggagcgau gcugguaagg ccauuucuuu ugcuaccaca uuggggguga      240

acaaaugcca uguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugccccau gcuggacgag ggaguggagc cagaugacgu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aacauaaaaa aggugaagca cgacgaucca      420

gaagagccgu gacgcuuccu ucucacucca caaggaagcu gcaaacgcga ucacagacuu      480

ggcuagaauc aagagaauac acaaagcacc ugaucaaggu ugagaauugg auauucagga      540

accccggguu ugcgcuagug gcuguagcua uugccuggcu ccugggaagc ucgacgagcc      600

aaaaagucau auacuuggua augauauugu ugauugcccc ggcauacagc aucaggugca      660

uaggaguuag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggggguugc gucaccguga uggcacagga caagccaaca guugacaucg      780

aguuggucac gacaacgguu agcaacaugg ccgagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccgcu guccaacaca aggugaagcc uaccuugaca      900

agcagucaga cacucaauau gucugcaaga gaacauuggu ggauagaggu uggggaaaug      960

gguguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg ugcuccaaga     1020

aaaugacagg caagagcauc cagccggaga acuuggagua ccggauaaug cuaucagugc     1080

auggauccca gcacaguggg augauuguga augacauagg acaugaaacu gacgaaaaca     1140

gagcaaaagu cgaggucaca cccaauucac caagagcaga agcaaccuug ggagguuuug     1200

gaagcuuggg acuugacugu gaaccaagga caggccuuga cuucucagau cuguauuacc     1260

ugaccaugaa caauaagcau ugguuggugc acaaggagug guuucaugac aucccauuac     1320

cuuggcaugc uggugcagac acuggaacuc cacacuggaa caacaaagag gcauuggugg     1380

aguuuaagga cgcccacgcc aagaggcaaa cuguuguggu ucuggggagc caagagggag     1440

cuguucacac ggcccucgcu ggagcuuugg aggcugagau ggauggugca aagggaaggc     1500

uauucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagguug aagggugugu     1560

cauauucccu guguaccgca guguguaccg cagcgaaggu cccagcugaa acauugcaug     1620

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag gucccagccc     1680

agauggcggu agacaugcag acccugaccc caguuggaag gcugauaacc gccaacccug     1740

ugaucacuga aaguacugag aauucaaaga ugauguugga gcucgaccca ccauuugggg     1800

auucuuacau ugucauagga gucggggaca agaaaaucac ccaucacugg caucggagug     1860

guagcaccau cggaaaggca uuugaagcca cugugagagg ugccaagaga auggcagucu     1920

ugggggacac agccugggac uuuggaucag uugggggugu guuuaauuca uuggguaagg     1980

guauucacca gaucuuugga gcagcuuuua aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcaca cuguuggugu gguuaggucu gaacacaaag aauggaucua     2100

ucucccucac augcuuagcc cuggggggag ugaugaucuu ccuuuccacg gcuguuucug     2160

cugauguugg gugcucggug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5390
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 2 wild type

<400>  5390
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaggag aggaagagac guggagcuga ugccagcauc ggaaucguca      120

gccuccugcu gacuacaguc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga caggagcgau gcugguaagg ccauuucuuu cguuaccaca cuggggguga      240

acaaaugcca ugugcagauc auggaccucg ggcauaugug ugacgccacc augaguuaug      300

agugccccau gcuggacgag ggaguggagc cagaugacgu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucauaaaaa aggugaagca cgacgaucca      420

gaagagccgu gacgcuuccu ucucacucua caaggaaguu gcaaacgcga ucgcagacuu      480

ggcuagaauc aagagaauac acaaagcacc ugaucaaggu ugagaauugg auauucagga      540

accccggguu ugcgcuagug gcuguagcua uugccuggcu ccugggaagc ucgacgagcc      600

aaaaagucau auacuugguc augauauugu ugauugcccc ggcauacagu aucaggugca      660

uaggaguuag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggagguugu gucaccguga uggcacagga caagccaaca guugacauag      780

aguuggucac gacaacgguu agcaacaugg ccgaggugag auccuacugc uacgaggcau      840

caauaucgga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaca      900

agcagucaga cacucaauau gucuguaaaa gaacauuggu ggacagaggu uggggaaaug      960

gguguggacu uuuuggcaag gggagcuugg ugacgugugc caaguuuaca ugcuccaaga     1020

aaaugacagg gaagagcauc cagccggaga acuuggagua ccggauaaug cuaucagugc     1080

auggauccca gcacaguggg augauuguga augacgaaaa cagagcaaaa gucgagguua     1140

cacccaauuc accaagagca gaagcaaccu ugggagguuu uggaagccug ggacuugauu     1200

gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug aacaauaagc     1260

auugguuggu gcacaaagag ugguuucaug acaucccauu accuuggcau ucuggugcag     1320

acacugaaac uccacacugg aacaacaaag aggcacuggu ggaguucaag gacgcccacg     1380

ccaagaggca aacuguugug guucugggga gccaagaagg agccguucac acggcucucg     1440

cuggagcucu ggaggcugag auggauggug cgaagggaag gcuauccuca ggccauuuga     1500

aaugccgccu aaaaauggac aagcuuaggu ugaagggugu gucauauucc cuguguaccg     1560

cagcguucac auucaccaag guuccagcug aaacauugca uggaacaguc acaguggagg     1620

ugcaguaugc agggagggau ggacccugca aggucccagc ccagauggcg guggacaugc     1680

agacccugac cccaguugga aggcugauaa cggcuaaccc ugugaucacu gaaagcacug     1740

agaauucaaa gaugauguug gagcucgacc caccauuugg ggauucuuac auugucauag     1800

gagucgggga caagaaaauc acccaucacu ggcaucggag ugguagcauc aucggaaagg     1860

cauuugaagc cacugugaga ggcgccaaga gaauggcagu cuugggagac acagccuggg     1920

acuuuggauc aguugggggu guguuuaacu cauugggcaa ggguauucac cagaucuuug     1980

gagcagcuuu caaaucacug uucggaggaa uguccugguu cucacagauc cucauaggca     2040

cacuguuggu gugguugggu cugaacacaa agaauggauc uaucucccuc acaugcuugg     2100

ccuugggagg agugaugauc uuccuuucca cggcuguuuc ugcugaugug ggguguucgg     2160

uggacuucuc aaagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5391
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 2 wild type

<400>  5391
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuaucaaugc uaggaaggag aggaagagac guggcgcuga caccagcauc ggaaucgucg      120

gccuccugcu gaccacagcc auggcagccg agaucacuag acgugggagu gcauacuaca      180

uguacuugga caggagcgau gcugguaagg ccauuucuuu ugccaccaca uuggggguga      240

acaaaugcca uguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugccccau gcuagacgag ggaguggagc cagaugacgu cgauugcugg ugcaacacga      360

caucgacuug gguuguguac ggaaccuguc aucauaaaaa aggugaagca cgacgaucca      420

gaagagccgu gacgcuuccu ucucacucca cgaggaagcu gcaaacgcga ucgcagacuu      480

ggcuagaguc aagagaauac acaaagcacc ugaucaaggu ugaaaauugg auauucagga      540

accccggguu ugcgcuagug gcuguagcua uagccuggcu ccugggaagc ucgacgagcc      600

aaaaaguuau auacuugguc augauauugu ugauugcccc ggcauacagc auuaggugca      660

uaggaguuag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggggguugc gucaccguga uggcacagga caagccaaca guugauaucg      780

aguuggucac gacaacgguu agcaacaugg ccgagguaag auccuacugc uaugaggcau      840

caauaucgga cauggcuucg gacagccguu guccaacaca aggugaagcc uaccuugaca      900

agcagucaga cacucaauau gucugcaaga gaacauuggu ggauagaggu uggggaaaug      960

gguguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg ugcuccaaga     1020

aaaugacagg caagagcauc cagccggaga acuuggagua ccggauaaug cuaucagugc     1080

auggauccca gcacaguggg augauuguga augacgaaaa cagagcaaaa gucgagguca     1140

cacccaauuc accaagagca gaagcaaccu ugggagguuu uggaagcuug ggacuugacu     1200

gugaaccaag gacaggccuu gacuucucag aucuguauua ccugaccaug aacaauaagc     1260

auugguuggu gcacaaggag ugguuucaug acaucccauu accuuggcau gcuggugcag     1320

acacuggaac uccacacugg aacaacaaag aggcauuggu ggaguucaag gacgcccacg     1380

ccaagaggca aacuguugug guucugggga gccaagaggg agcuguucau acggcccucg     1440

cuggagcuuu ggaggcugag auggauggug caaagggaag gcuauucucu ggccauuuga     1500

aaugccgccu aaaaauggac aagcuuaggu ugaagggugu gucauauucc cuguguaccg     1560

cagcguucac auuuaccaag gucccagcug aaacauugca uggaacaguc acaguggagg     1620

ugcaguaugc agggacagac ggacccugca aagucccagc ccagauggcg guggacaugc     1680

agacccugac cccaguugga aggcugauaa ccgccaaucc ugugaucacu gaaaguacug     1740

agaauucaaa gaugauguug gagcucgacc caccauuugg ggauucuuac auugucauag     1800

gagucgggga caagaaaauc acccaucacu ggcaucggag ugguagcacc aucggaaagg     1860

cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggggac acagccuggg     1920

acuuuggauc aguugggggu guguuuaauu cauuggguaa ggguauucac cagaucuuug     1980

gagcagcuuu caaaucacug uuuggaggaa uguccugguu cucacagauc cucauaggca     2040

cacuguuggu gugguuaggu uugaacacaa agaauggauc uaucucccuc acaugcuuag     2100

cccugggggg agugaugauc uuccuuucca cggcuguuuc ugcugauguu gggugcucgg     2160

uggacuucuc aaagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5392
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 2 wild type

<400>  5392
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca uugggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugagaca cggcgaucua      420

gaagaucugu gucgcuccgu uaucacuaua caaggaaguu gcaaacgcgg ucgcagacau      480

gguuagaauc aagagaauac aagaagcacu ugaucauggu cgaaaacugg auauucagga      540

accccggguu ugccauagug uccguugcca uuaccuggcu gaugggaagc uugacgagcc      600

aaaaagucau auacuugguc augauagugu ugauuguccc ggcauacagu aucagcugca      660

uuggagucag caauagagac uuaguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggagggugc guuaccgaga uggcacagga caagccaaca guugacauag      780

aguuggucac gaugacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cguuauccga cauggcuucg gccagucguu gcccaacaca aggcgaaccc ucccucgaca      900

agcaaucaga cacucaaucu guaugcaaaa gaacauuagg agacagaggu uggggaaaug      960

guugugggau uuuuggcaaa gggagcuugg ugacauguuc caaguucacg uguuguaaga     1020

agaugcccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cucccagugc     1080

auggcuccca gcauagcggg augauuguga augacauagg acaugaaacu gacgaaaaca     1140

gagcgaaagu cgaggucaca cccaauucac caagagcaga agcaaccuug ggagguuuug     1200

gaagcuuggg acuugacugu gaaccaagga caggccuuga cuucucagau cuguauuauc     1260

ugaccaugaa caacaagcau ugguuggugc acaaggagug guuucaugac aucccauuac     1320

cuuggcaugc uggugcagac acuggaacuc cacacuggaa caacaaagag gcauuggugg     1380

aguucaagga cgcccacgcc aagaggcaaa cuguuguggu ucuggggagc caagagggag     1440

cuguucacac ggcccucgcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1500

uauucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagguug aagggugugu     1560

cauauucccu guguacugca gcguucacau uuaccaaggu cccagcugaa acauugcaug     1620

gaacaguuac aguggaggug cagucugcag ggacagaugg acccugcaag gucccagccc     1680

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaaccccg     1740

ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca ccauuugggg     1800

auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg cauaggagug     1860

guagcaccau cggaaaggca uuugaagcca cugugagagg cgccaagaga auggcagucc     1920

ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca cuggguaagg     1980

gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug uccugguucu     2040

cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag aauggaucua     2100

ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg gcuguuucug     2160

cugacguggg gugcucagug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5393
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 2 wild type

<400>  5393
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc uaggaaagag aggaagagac guggcgcaga caccagcauc ggaaucauug      120

gccuccugcu gacuacagcc auggcagcag agaucacuag acgcgggagu gcauacuaca      180

uguacuugga uaggagcgau gccgggaagg ccauuucguu ugcuaccaca augggaguga      240

acaagugcca cguacagauc auggaccucg ggcacaugug ugacgccacc augaguuaug      300

agugcccuau gcuggaugag ggaguggaac cagaugaugu cgauugcugg ugcaacacga      360

caucaacuug gguuguguac ggaaccuguc aucacaaaaa aggugaggca cggcccucaa      420

gaagagcugu cacgcuuccu ucccauucca caagaaaguu acagacacgg ucacaaaccu      480

ggcuugaauc acgggaguac acgaagcacc ugaucaaagu ugagaacugg auacuccgga      540

auccaggguu cacaguugcu gcucuagcaa uggccuggcu cuugggaaau uccacgagcc      600

agcgagugau cuaucuggug augauccugu ugauugcccc ggcauacagu aucaggugca      660

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      720

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      780

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      840

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      900

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      960

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga     1020

agaugaccgg gaagagcauu caaccggaga auuuggagua cagaauuaug cugucagugc     1080

acggcuccca acacagcggg augauuguga augacauagg acaugagacc gaugaaaaca     1140

gagcaaaagu ggaggucaca ccaaacucac caagagcuga agcgacccua gggggcuucg     1200

ggaguuuggg acucgauugu gagccaagga ccgguuuaga uuucuccgac cuguacuauu     1260

uaaccaugaa caacaagcau ugguuggugc acaaggagug guuccacgac auuccauugc     1320

cauggcacgc uggagcggac acagggacuc cccauuggaa caacaaagag gcauugguug     1380

aguucaagga ugcccacgcc aaaagacaga cuguugucgu guugggaagu caggagggug     1440

ccguccacac agcccuggca ggagcuuugg aagcggagau ggauggagcc aaaggaaggc     1500

uguucucagg ucauuugaag ugccggauaa agauggacaa gcuuagguug aagggugucu     1560

cauacucauu gugcacugcc gccuucacau ucucaaaggu uccggcugaa acgcugcaug     1620

ggacagucac aguugaggug caguaugcgg gcagagaugg accuugcaag gugccagccc     1680

aaauggcagu ggauaugcag acucuuaccc cagugggaag guugauaacg gcaaauccca     1740

ucauaacuga gagcacugaa aacucgagaa ugauguugga gcuggaccca cccuuuggag     1800

acucuuacau ugucauuggg guuggagaua agaaaauuac ccaucauugg cacagaagug     1860

gaagcacgau ugggaaggca uuugaggcca cugugagagg ugcaaaaagg auggcagucc     1920

ugggagauac cgccugggau uuugguucug uuggaggcgu guucaauucu cugggcaagg     1980

gaguucauca ggucuucgga gcagccuuua aauccuuauu uggcggaaug uccugguuuu     2040

cacaaauucu uauuggcaca cuuuuagugu gguugggcuu gaauacaaaa aauggaucca     2100

uuucccuugc augucuggcg cuuggagggg cgaugaucuu ucucuccacg gccgucucgg     2160

cagacguggg gugcucaguu gacuucugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  5394
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 2 wild type

<400>  5394
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5395
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 2 wild type

<400>  5395
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac acgugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac uguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaaucu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaacg     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5396
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 2 wild type

<400>  5396
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau uggauaugaa acugacgaag     1080

auagagcgaa agucgagguu acgccuaauu caccaagagc ggaagcaacc uugggaggcu     1140

uuggaagcuu aggacuugac ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu     1200

accugaccau gaacaauaag cauugguugg ugcacaaaga gugguuucau gacaucccau     1260

ugccuuggca ugcuggggca gacaccggaa cuccacacug gaacaacaaa gaggcauugg     1320

uagaauucaa ggaugcccac gccaagaggc aaaccgucgu cguucugggg agccaggaag     1380

gagccguuca cacggcucuc gcuggagcuc uagaggcuga gauggauggu gcaaagggaa     1440

ggcuguucuc uggccauuug aaaugccgcc uaaaaaugga caagcuuaga uugaagggcg     1500

ugucauauuc cuugugcacu gcggcauuca cauucaccaa ggucccagcu gaaacacugc     1560

auggaacagu cacaguggag gugcaguaug cagggacaga uggacccugc aagaucccag     1620

uccagauggc gguggacaug cagacccuga ccccaguugg aaggcugaua accgccaacc     1680

ccgugauuac ugaaagcacu gagaacucaa agaugauguu ggagcuugac ccaccauuug     1740

gggauucuua cauugucaua ggaguugggg acaagaaaau cacccaccac uggcauagga     1800

gugguagcac caucggaaag gcauuugagg ccacugugag aggcgccaag agaauggcag     1860

uccuggggga uacagccugg gacuucggau cagucggggg uguguucaac ucacugggua     1920

agggcauuca ccagauuuuu ggagcagccu ucaaaucacu guuuggagga auguccuggu     1980

ucucacagau ccucauaggc acgcugcuag ugugguuagg uuugaacaca aagaauggau     2040

cuaucucccu cacaugcuug gcccuggggg gagugaugau cuuccucucc acggcuguuu     2100

cugcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5397
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 2 wild type

<400>  5397
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5398
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 2 wild type

<400>  5398
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau ugucaaugau acaggauaug     1080

aaacugacga aaauagagcg aaagucgagg uuacgccuaa uucaccaaga gcggaagcaa     1140

ccuugggagg cuuuggaagc uuaggacuug acugugaacc aaggacaggc cuugacuuuu     1200

cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa gagugguuuc     1260

augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaggcauu gguagaauuc aaggaugccc acgccaagag gcaaaccguc gucguucugg     1380

ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu gagauggaug     1440

gugcaaaggg aaagcuguuc ucuggccauu ugaaaugccg ccuaaaaaug gacaagcuua     1500

gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc aaggucccag     1560

cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca gauggacccu     1620

gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu ggaaggcuga     1680

uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug uuggagcuug     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa aucacccacc     1800

acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug agaggcgcca     1860

agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg gguguguuca     1920

acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacgcugcu agugugguua gguuugaaca     2040

caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug aucuuccucu     2100

ccacggcugu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5399
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 2 wild type

<400>  5399
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucgc aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacaguuga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau ugucaaugau acaggauaug     1080

aaacugacga aaauagagcg aaagucgagg uuacgccuaa uucaccaaga gcggaagcaa     1140

ccuugggagg cuuuggaagc uuaggacuug acugugaacc aaggacaggu cuugacuuuu     1200

cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa gagugguuuc     1260

augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaggcauu gguagaauuc aaggaugccc acgccaagag gcaaaccguc gucguucugg     1380

ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu gagauggaug     1440

gugcaaaggg aaagcuguuc ucuggccauu ugaaaugccg ccuaaaaaug gacaagcuua     1500

gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc aaggucccag     1560

cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca gauggacccu     1620

gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu ggaaggcuga     1680

uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug uuggagcuug     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa aucacccacc     1800

acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug agaggcgcca     1860

agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg gguguguuca     1920

acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacgcugcu agugugguua gguuugaaca     2040

caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug aucuuccucu     2100

ccacggcugu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5400
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 2 wild type

<400>  5400
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacaguuga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugac ugucaaugau auaggauaug     1080

aaacugacga aaauagagcg aaagucgagg uuacgccuaa uucaccaaga gcggaagcaa     1140

ccuugggagg cuuuggaagc uuaggacuug acugugaacc aaggacaggc cuugacuuuu     1200

cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa gagugguuuc     1260

augacauccc auugccuugg caugcugggg cagacacugg aacuccacac uggaacaaca     1320

aagaggcauu gguagaauuc aaggaugccc acgccaagag gcaaaccguc gucguucugg     1380

ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu gagauggaug     1440

gugcaaaggg aaagcuguuc ucuggccauu ugaaaugccg ccuaaaaaug gacaagcuua     1500

gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc aaggucccag     1560

cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca gauggacccu     1620

gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu ggaaggcuga     1680

uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug uuggagcuug     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa aucacccacc     1800

acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug agaggcgcca     1860

agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg gguguguuca     1920

acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacgcugcu agugugguua gguuugaaca     2040

caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug aucuuccucu     2100

ccacggcugu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5401
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 2 wild type

<400>  5401
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cguuggccuc cuguugacua cagccauggc agcagagauc acuagacgug      120

ggagugcaua cuacauguac uuggauagga gcgaugcagg gaaggccauu ucuuucgcua      180

ccacauuggg ggugaacaaa ugccaugugc agaucaugga ccucgggcac augugugacg      240

ccaccaugag cuaugaaugc ccuaugcugg acgagggggu ggaaccagau gacgucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucau aaaaaaggug      360

aagcacggcg aucuagaaga gccgucacgc ucccaucuca cuccacaagg aaauugcaaa      420

cgcggucgca gacuuggcua gaaucaagag aauacacaaa gcaccugauc aagguugaaa      480

auuggauauu caggaacccu gguuuuacgc uaguggcugu cgccaucgcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauagga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga cguuguccug gaacauggag gcugcgucac cgugauggca caggacaagc      720

caacaguuga cauagagcug gucacaacaa cgguuaguaa cauggccgag gugagauccu      780

auuguuacga ggcaucaaua ucggacaugg cuucggacag ucgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauauguuug caaaagaaca uugguggaca      900

gagguugggg aaaugggugu ggacucuuug gcaaagggag uuuggugaca ugugcuaagu      960

ucacgugcuc caagaagaug acugggaaga gcauucagcc ggagaaccug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaca gugggaugau uguuaaugau gaaaacagag     1080

cgaaggucga gguuacgccc aauucaccaa gagcagaagc aacccuggga ggcuuuggaa     1140

gcuuaggacu ugauugugaa ccaaggacag gccuugacuu uucagaucug uauuaccuaa     1200

ccaugaauaa caagcauugg uuggugcaca aagagugguu ucaugacauc ccauugcccu     1260

ggcaugcugg ggcagacacu ggaacuccac auuggaacaa caaggaggca uuaguggaau     1320

ucaaggacgc ccacgccaag aggcaaaccg ucgugguuuu ggggagccag gaaggagccg     1380

uccacacggc ucuugcugga gcucuagagg cugagaugga uggugcaaag ggaaggcuau     1440

ucucuggcca cuugaaaugu cgcuuaaaaa uggacaagcu uagauugaag ggcgugucau     1500

auuccuugug caccgcggca uucacauuca ccaagguccc ggcugaaaca cuacauggaa     1560

cagucacagu ggaggugcag uaugcaggga cagauggacc cugcaagguc ccagcccaga     1620

uggcggugga caugcagacc uugaccccag ucggaaggcu gauaaccgcc aaccccguga     1680

uuacugaaag cacugagaau ucaaagauga uguuggagcu cgacccacca uuuggggauu     1740

cuuacauugu cauaggaguu ggggauaaga aaaucaccca ucacuggcau aggaguggca     1800

gcaccauugg aaaagcauuu gaagccacug ugagaggcgc uaagagaaug gcaguccugg     1860

gggacacagc uugggacuuu ggaucagucg gagguguguu uaacucauug ggcaagggca     1920

uucaucagau uuuuggagca gcuuucaaau cacuguuugg aggaaugucc ugguucucac     1980

agauccucau aggcacucug cugguguggu uaggucugaa cacaaagaau gggucuaucu     2040

cccucacaug cuuagcccug gggggaguga ugaucuuccu cuccacggcu guuucugcuu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  5402
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 2 wild type

<400>  5402
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaccgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau uggauaugaa acugacgaaa     1080

auagagcgaa agucgagguu acgccuaauu caccaagagc ggaagcaacc uugggaggcu     1140

uuggaagcuu aggacuugac ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu     1200

accugaccau gaacaauaag cauugguugg ugcacaaaga gugguuucau gacaucccau     1260

ugccuuggca ugcuggggca gacaccggaa cuccacacug gaacaacaaa gaggcauugg     1320

uagaauucaa ggaugcccac gccaagaggc aaaccgucgu cguucugggg agccaggaag     1380

gagccguuca cacggcucuc gcuggagcuc uagaggcuga gauggauggu gcaaagggaa     1440

agcuguucuc uggccauuug aaaugccgcc uaaaaaugga caagcuuaga uugaagggcg     1500

ugucauauuc cuugugcacu gcggcauuca cauucaccaa ggucccagcu gaaacacugc     1560

auggaacagu cacaguggag gugcaguaug cagggacaga uggacccugc aagaucccag     1620

uccagauggc gguggacaug cagacccuga ccccaguugg aaggcugaua accgccaacc     1680

ccgugauuac ugaaagcacu gagaacucaa agaugauguu ggagcuugac ccaccauuug     1740

gggauucuua cauugucaua ggaguugggg acaagaaaau cacccaccac uggcauagga     1800

gugguagcac caucggaaag gcauuugagg ccacugugag aggcgccaag agaauggcag     1860

uccuggggga uacagccugg gacuucggau cagucggggg uguguucaac ucacugggua     1920

agggcauuca ccagauuuuu ggagcagccu ucaaaucacu guuuggagga auguccuggu     1980

ucucacagau ccucauaggc acgcugcuag ugugguuagg uuugaacaca aagaauggau     2040

cuaucucccu cacaugcuug gcccuggggg gagugaugau cuuccucucc acggcuguuu     2100

cugcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5403
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 2 wild type

<400>  5403
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caggggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5404
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 2 wild type

<400>  5404
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuaga aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggccucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5405
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 2 wild type

<400>  5405
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cuacugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acugguacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5406
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 2 wild type

<400>  5406
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccacuaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaua     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca cgcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5407
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 2 wild type

<400>  5407
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag auaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguggaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcugc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5408
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 2 wild type

<400>  5408
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucu agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5409
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 2 wild type

<400>  5409
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuacgcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccagcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug gucacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagu accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5410
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 2 wild type

<400>  5410
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuagaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggacu gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucc uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagucgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5411
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 2 wild type

<400>  5411
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca gcuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggccgca aaccugguug gaaucaagag aauacacaaa gcauuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac aagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5412
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 2 wild type

<400>  5412
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaaguucaac gagcaaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug ggacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5413
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 2 wild type

<400>  5413
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gcgccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuagaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggacu gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucc uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5414
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 2 wild type

<400>  5414
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau cguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacaugggg guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc uuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggacucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5415
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 2 wild type

<400>  5415
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccaccagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcauuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac aagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagccucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucugacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5416
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 2 wild type

<400>  5416
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggauagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gccgugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agaaucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugauaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcccagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5417
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 2 wild type

<400>  5417
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugagugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauacu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauauu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuca     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggaaucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5418
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 2 wild type

<400>  5418
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuucaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaacaaua ucagacauag cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacugcc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5419
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 2 wild type

<400>  5419
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccaccagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucugacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccgaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg aguuacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5420
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 2 wild type

<400>  5420
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgc uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

auagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

ccuugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca uugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggugcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucaucg gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5421
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 2 wild type

<400>  5421
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

guguaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaaaaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuagga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc uuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5422
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 2 wild type

<400>  5422
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuacauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcc uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaagcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgccaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cucaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5423
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 2 wild type

<400>  5423
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa aagaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gccgugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu gguuucgcuu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauauguuug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaauguuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcauag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5424
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 2 wild type

<400>  5424
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuacauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc uuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggaagcac augcuaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgccaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cucaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5425
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 2 wild type

<400>  5425
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugcgaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa uacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagccg     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccuguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa acucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5426
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 2 wild type

<400>  5426
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc accagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uugguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcauugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcaggggca gauggacccu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgccaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5427
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 2 wild type

<400>  5427
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gccgugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu gguuucgcuu uagcagcagc ugccaucgcg uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gcccuggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaauguuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcccagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5428
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 2 wild type

<400>  5428
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcagagguc acuagacgug      120

ggagugcaua cuacauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg uuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuucu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgccaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cucaugugcu uggccuuagg gggagugcug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5429
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 2 wild type

<400>  5429
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuacauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccauagcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga aucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aguaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaauc cuaggacugg auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgccaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cucaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5430
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 2 wild type

<400>  5430
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggaaguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac acgugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccauuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcaugggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaacg     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5431
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 2 wild type

<400>  5431
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuacauguac uuggacagaa acgaugcugg ggaggccaua ucuuucccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacagag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa guuauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag gcugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacaccc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacugg auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgccaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gauacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cucaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5432
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 2 wild type

<400>  5432
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugccgg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaag      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

auagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacuguuga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag cuuggugaca ugcgcuaagu      960

uugcaugcuc caagaagaug accgggaaga gcauccagcc agagaauuug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaacagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaaa gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggugcucuua     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5433
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 2 wild type

<400>  5433
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagccauggc agcggaaguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacacuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu agaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaccac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uggcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucucc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagagg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccgguaauc acugaaagca cagagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaua     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5434
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 2 wild type

<400>  5434
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacacuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu agaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag auccagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucaguaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaacaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacacugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaggca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcguucuua     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaua     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cuccgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5435
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 2 wild type

<400>  5435
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacacuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu agaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gagucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagauaccc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gaaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguccauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gacaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggacccu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagu accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggccugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5436
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 2 wild type

<400>  5436
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagccauggc aguggagguc acuagacgug      120

ggaaugcaua cuauauguac uuggacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacaauggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu agaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaccac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauacacaaa gcaccugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guuguguuac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcuugcuc uaagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugau acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg caugcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcagacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacu aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccuguaauc acugaaagca cugagaacuc caagaugaug cuggaacugg     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggaaaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguuggg ggugcucuca     1920

acucacuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu ggugugguug ggucugaaua     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5437
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 2 wild type

<400>  5437
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagccauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacacuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu agaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaccac aaaaaaggug      360

aagcgcggag aucuagaaga gcugugacgc uccccuccca cuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uggcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

auagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga ugucgucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcagag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgccaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgaa     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggagg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggaugcac augccaaaag gcaaacuguc gugguccuag     1380

ggagucaaga gggagcaguu cacacggcuc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gacugaaggg cgugucauac ucccugugua ccgcagcguu cacauucacu aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccuguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caaggguauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaua     2040

caaagaaugg aucuauuucc cuugugugcu uggccuuagg gggaguguug aucuucuugu     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5438
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 2 wild type

<400>  5438
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagccauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacacuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu agaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaccac aaaaaaggug      360

aagcgcggag aucuagaaga gcugugacgc uccccuccca cuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uggcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

auagcaucag gugcauagga gucaacaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga ugucgucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcagag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgccaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgaa     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggagg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggaugcac augccaaaag gcaaacuguc gugguccuag     1380

ggagucaaga gggagcaguu cacacggcuc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gacugaaggg cgugucauac ucccugugua ccgcagcguu cacauucacu aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccuguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caaggguauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaua     2040

caaagaaugg aucuauuucc cuugugugcu uggccuuagg gggaguguug aucuucuugu     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5439
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 2 wild type

<400>  5439
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagccauggc aguggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacacuggg gaugaacaag uguuacauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuauguugg augagggggu agaaccagau gacgucgauu      300

guuggugcaa cacgacauca acuuggguug uguacggaac cugccaccac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauauacaaa gcaccugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcc uggcuuuugg      540

gaaguucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guuguguuac cguaauggca caggacaaac      720

cggcugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

auugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcag ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gcugaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg caugcugggg cagacaccgg aacuccacau uggaacaaca     1320

aagaagcauu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggacccu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggaggcuga     1680

uaaccgcuaa cccuguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccauc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggauuuugg aucaguugga ggugcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu ggugugguug ggucugaaua     2040

caaagaaugg aucuauuucc cuuacgugcu uggccuuagg gggaguguug aucuuuuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5440
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 2 wild type

<400>  5440
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gcgucggaau uguuggccuc cuccugacca cagccauggc aguagagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacacuggg gaugaauaag uguuacauac aaaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuauguugg augagggggu agaaccagau gacgucgauu      300

gcuggugcaa cacgacauca acuuggguug uguauggaac cugccaccac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauacacaaa gcaccugauu agaguugaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugucaucgcu uggcuuuugg      540

gaaguucaac gagccaaaaa gucauauauc uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guuguguuac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggauaugg cuucggacag ccgcugccca acacaaggug      840

aggccuaccu ugacaagcag ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuugggagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuaccugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg caugcugggg cagacacugg aacuccacau uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augcaaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gagccaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugca cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaua ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccuguaauc acugaaagca ccgagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccauc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguuggg ggugcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguucggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu ggugugguug ggucugaaua     2040

caaagaaugg aucuauuucc cuuacgugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5441
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 2 wild type

<400>  5441
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagccauggc aguggagguc accagacgug      120

ggagugcaua cuauauguac uuagacagaa gcgaugcugg ggaggccaua ucuuuuccaa      180

ccacacuggg ggugaauaag uguuacauac agaucaugga ucuuggacac augugugaug      240

ccacaaugag cuaugaaugc ccuauguugg augagggggu agaaccagau gacgucgauu      300

gcuggugcaa cacgacaucg acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aggcacggag aucuagaaga gcugugacgc uccccucuca uuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauacacaaa gcacuugauc agagucgaaa      480

auuggauauu caggaacccu ggcuuugcgu uggcagcagc ugccauugcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acuguugauu gccccggcau      600

acaguaucag gugcauagga gucagcaaua gggauuuugu ggaagguaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag guuguguuac cguaauggca caggacaagc      720

caacuguuga uauagaguug gucacaacaa cgguuagcaa cauggcggag guaagauccu      780

acugcuacga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcag ucagacacuc aauauguuug caaaagaacg uuaguggaca      900

gagguugggg aaauggaugu ggacucuuug gcaaagggag ccuggugaca ugcgccaagu      960

uugcaugcuc caagaaaaug acugggaaga gcauccagcc agagaaccug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau uguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggagg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcauugguu ggugcacaag gagugguucc     1260

augacauucc acuaccuugg caugcugggg cagacaccgg aacuccacau uggaacaaca     1320

aagaagcauu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagccguu cacacggcuc uugcuggagc ccuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg cuugaaaaug gacaaacuua     1500

gauugaaggg cgugucauac uccuuaugua ccgcggcguu cacauucacc aagaucccgg     1560

cugaaacgcu gcaugggaca gucacagugg agguacagua ugcagggaca gauggacccu     1620

gcaagguucc agcucagaug gcgguggaua ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccugugauc acugaaagca cugagaauuc aaagaugaug uuggaacuug     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggauaagaag aucacccacc     1800

acugguacag gaguggcagc accaucggaa aagcauuuga agccacugug agaggcgcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucagucgga ggugcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauag gaacguugcu ggugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuacgugcu uggccuuagg gggaguguug aucuuccuau     2100

cuacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5442
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 2 wild type

<400>  5442
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagccauggc aguggagguc accagacgug      120

ggagugcaua cuauauguac uuagacagaa gcgaugcugg gaaggccaua ucuuuuccaa      180

ccacacuggg ggugaauaag uguuacauac agaucaugga ucuuggacac augugugaug      240

ccacaaugag cuaugaaugc ccuauguugg augagggggu agaaccagau gacgucgauu      300

gcuggugcaa cacgacaucg acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aggcacggag aucuagaaga gcugugacgc uccccucuca uuccacuagg aagcugcaaa      420

cgcggucgca gaccugguug gaaucaagag aauacacaaa gcacuugauc agagucgaaa      480

auuggauauu caggaacccu ggcuuugcgu uggcagcagc ugccauugcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acuguugauu gccccggcau      600

acaguaucag gugcauagga gucagcaaua gggauuuugu ggaagguaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag guuguguuac cguaauggca caggacaagc      720

caacuguuga uauagaguug gucacaacaa cgguuagcaa cauggcggag guaagauccu      780

acugcuacga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcag ucagacacuc aauauguuug caaaagaacg uuaguggaca      900

gagguugggg aaauggaugu ggacucuuug gcaaagggag ccuggugaca ugcgccaagu      960

uugcaugcuc caagaaaaug acugggaaga gcauccagcc agagaaccug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau uguuaaugac auaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggagg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcauugguu ggugcacaag gagugguucc     1260

augacauucc acuaccuugg caugcugggg cagacaccgg aacuccacau uggaacaaca     1320

aagaagcauu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagccguu cacacggcuc uugcuggagc ccuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg cuugaaaaug gacaaacuua     1500

gauugaaggg cgugucauac uccuuaugua ccgcggcguu cacauucacc aagaucccgg     1560

cugaaacgcu gcaugggaca gucacagugg agguacagua ugcagggaca gauggacccu     1620

gcaagguucc agcucagaug gcgguggaua ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccugugauc acugaaagca cugagaauuc aaagaugaug uuggaacuug     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggauaagaag aucacccacc     1800

acuggcacag gaguggcagc accaucggaa aagcauuuga agccacugug agaggcgcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucagucgga ggugcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauag gaacguugcu ggugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuacgugcu uggccuuagg gggaguguug aucuuccuau     2100

cuacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5443
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 2 wild type

<400>  5443
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cguuggccuc cuguugacua cagccauggc agcagagauc acuagacgug      120

ggagugcaua cuacauguac uuggauagga gcgaugcagg gaaggccauu ucuuucgcua      180

ccacauuggg ggugaacaaa ugccaugugc agaucaugga ccucgggcac augugugacg      240

ccaccaugag cuaugaaugc ccuaugcugg acgagggggu ggaaccagau gacgucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucau aaaaaaggug      360

aagcacggcg aucuagaaga gccgucacgc ucccaucuca cuccacaagg aaauugcaaa      420

cgcggucgca gacuuggcua gaaucaagag aauacacaaa gcaccugauc aagguugaaa      480

auuggauauu caggaacccu gguuuuacgc uaguggcugu cgccaucgcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauagga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga cguuguccug gaacauggag gcugcgucac cgugauggca caggacaagc      720

caacaguuga cauagagcug gucacaacaa cgguuaguaa cauggccgag gugagauccu      780

auuguuacga ggcaucaaua ucggacaugg cuucggacag ucgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauauguuug caaaagaaca uugguggaca      900

gagguugggg aaaugggugu ggacucuuug gcaaagggag uuuggugaca ugugcuaagu      960

ucacgugcuc caagaagaug acugggaaga gcauucagcc ggagaaccug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaca gugggaugau uguuaaugac acaggacaug     1080

aaacugauga aaacagagcg aaggucgagg uuacgcccaa uucaccaaga gcagaagcaa     1140

cccugggagg cuuuggaagc uuaggacuug auugugaacc aaggacaggc cuugacuuuu     1200

cagaucugua uuaccuaacc augaauaaca agcauugguu ggugcacaaa gagugguuuc     1260

augacauccc auugcccugg caugcugggg cagacacugg aacuccacau uggaacaaca     1320

aggaggcauu aguggaauuc aaggacgccc acgccaagag gcaaaccguc gugguuuugg     1380

ggagccagga aggagccguc cacacggcuc uugcuggagc ucuagaggcu gagauggaug     1440

gugcaaaggg aaggcuauuc ucuggccacu ugaaaugucg cuuaaaaaug gacaagcuua     1500

gauugaaggg cgugucauau uccuugugca ccgcggcauu cacauucacc aaggucccgg     1560

cugaaacacu acauggaaca gucacagugg aggugcagua ugcagggaca gauggacccu     1620

gcaagguccc agcccagaug gcgguggaca ugcagaccuu gaccccaguc ggaaggcuga     1680

uaaccgccaa ccccgugauu acugaaagca cugagaauuc aaagaugaug uuggagcucg     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggauaagaaa aucacccauc     1800

acuggcauag gaguggcagc accauuggaa aagcauuuga agccacugug agaggcgcua     1860

agagaauggc aguccugggg gacacagcuu gggacuuugg aucagucgga gguguguuua     1920

acucauuggg caagggcauu caucagauuu uuggagcagc uuucaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacucugcu ggugugguua ggucugaaca     2040

caaagaaugg gucuaucucc cucacaugcu uagcccuggg gggagugaug aucuuccucu     2100

ccacggcugu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5444
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 2 wild type

<400>  5444
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggucuc cugcugacua cagccauggc agcggagauu accagacgug      120

gaagugcaua cuacauguac uuggacagga gcgaugcugg gaaggccauc uccuuugcua      180

ccaacuuggg aguuaacaaa ugccauguac agaucaugga ucucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuauguugg acgagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aagcacggcg aucuagaaga gcugugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcaccugauc aagguugaaa      480

acuggauauu uaggaacccc ggguuugcgc ucgcagcugu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu ugguuaugau acugcugauu gccccggcau      600

acagcaucag gugcauugga gucagcaaua gagacuucgu ggaaggcaug ucagguggga      660

ccuggguuga ugucgucuug gaacauggag guugcgucac cguuauggca caggacaagc      720

caacaguuga cauagaguug gucacgacaa cgguuaguaa cauggccgag guaagauccu      780

acuguuauga ggcaucaaua ucagacaugg cuucggacag ucgcugccca acacaaggug      840

aagccuaccu ugauaagcaa ucagacaccc aauaugugug caaaagaaca uugguggaca      900

gagguugggg aaaugggugu ggacuuuuug gcaaagggag ccuggugaca ugugccaagu      960

ucacguguuc caagaagaug acugggaaga gcauucagcc ggagaaucug gaguaucgaa     1020

uaaugcuauc agugcauggc ucccagcaca gcgggaugau uguuaaugac auaggacaug     1080

aaacugacga aaacagagcg aaagucgagg ucacgcccaa uucaccaaga gcagaagcaa     1140

ccuugggagg cuuuggaagc uuaggacuug auugugaacc aaggacaggc cuggacuuuu     1200

cagauuugua cuacuugacc augaacaaca agcacugguu ggugcacaaa gagugguuuc     1260

augacauccc auugccuugg caugcugggg cagauaccgg aacuccacac uggaacaaca     1320

aggaggcauu gguagaguuc aaagaugccc acgccaagag gcaaaccguc guuguucuag     1380

ggagccagga aggagccguu cacacggcuc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaagg aaggcuguuc uccggccauu ugaaaugccg cuuaaaaaug gacaagcuua     1500

gauugaaggg cgugucauau uccuugugca ccgcagcguu cacauuuacc aaggucccag     1560

cugaaacauu gcauggaaca guuacagugg aggugcagua ugcagggaca gauggacccu     1620

gcaagguccc agcccagaug gcgguggaca ugcagacccu gaccccaguc gggaggcuga     1680

uaaccgccaa cccugugauu acugaaagca cugagaauuc aaagaugaug uuggagcucg     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa aucacccauc     1800

acuggcauag gagugguagc accaucggaa aagcauuuga ggccacugug agaggugcca     1860

agagaauggc aguucugggg gauacagccu gggacuucgg aucagucggg ggcguguuca     1920

acucauuggg caaggguguu caccagauuu uuggagcagc uuucaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacgcugcu ggugugguug gguuugaaca     2040

caaagaaugg auccaucucc cucacaugcu uggcccuggg gggagugaug aucuuccucu     2100

ccacggcugu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5445
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 2 wild type

<400>  5445
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacaguuga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaaugguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

ucacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau ugucaaugau auaggacaug     1080

aaacugacga aaacagagcg aaagucgagg uuacgccuaa uucaccaaga gcggaagcaa     1140

ccuugggagg cuuuggaagc uuaggacuug acugugaacc aaggacaggc cuugacuuuu     1200

cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa gagugguuuc     1260

augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaggcauu gguagaauuc aaggaugccc acgccaagag gcaaaccguc gucguucugg     1380

ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu gagauggaug     1440

gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug gacaagcuua     1500

gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc aaggucccag     1560

cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca gauggacccu     1620

gcaagguccc agcccagaug gcgguggaca ugcagacccu gaccccaguu ggaaggcuga     1680

uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug uuggagcuug     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa aucacccacc     1800

acuggcauag gagugguagc accaucggaa aggcauuuga agccacugug agaggcgcca     1860

agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg gguguguuca     1920

acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacgcugcu agugugguua gguuugaaca     2040

caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug aucuuccucu     2100

ccacggcugu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5446
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 2 wild type

<400>  5446
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggucuc cugcugacua cagccauggc agcagagauu accagacgug      120

gaagugcaua cuacauguac uuggacagga gcgaugcugg gaaggccauc uccuuugcua      180

ccaauuuggg aguuaacaaa ugccauguac agaucaugga ucucgggcac augugugacg      240

ccaccaugag uuaugagugu ccuauguugg acgagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aagcacggcg aucuagaaga gcugugacgc ucccuucuca cucuacaagg aagcugcaaa      420

cgcggucgca gacuugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc ucgcagcugu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauugga gucagcaaua gagacuucgu ggaaggcaug ucagguggga      660

ccuggguuga ugucgucuug gaacauggag guugcgucac cguaauggca caggacaagc      720

caacaguuga cauagaguug gucacgacaa cggucaguaa cauggccgag guaagauccu      780

acuguuauga ggcaucaaua ucggacaugg cuucggacag ucgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauauguaug caaaagaaca uugguggaca      900

gagguugggg aaaugggugu ggacuuuuug gcaaagggag ccuggugaca ugugccaagu      960

ucacguguuc caagaagaug accgggaaga gcauucagcc ggagaaucug gaguaucgaa     1020

uaaugcuauc agugcauggc ucccagcaca gcgggaugau uguuaaugac gaaaauaggg     1080

cgaaagucga ggucacgccc aauucaccaa gagcagaagc aaccuuggga ggcuuuggaa     1140

gcuuaggacu ugauugugaa ccaaggacag gccuggacuu uucagauuug uacuacuuga     1200

ccaugaacaa uaagcacugg uuagugcaca aagagugguu ucaugacauc ccauugcccu     1260

ggcaugcugg ggcagacacc ggaacuccac auuggaacaa caaggaggca uugguagagu     1320

ucaaagaugc ccacgcuaag aggcaaaccg ucguuguucu agggagccag gaaggagccg     1380

uucacacggc ucuugcugga gcucuggagg cugagaugga uggugcaaaa ggaagguugu     1440

ucuccggcca uuugaaaugc cgcuuaaaaa uggacaagcu uagauugaag ggcgugucau     1500

auuccuugug caccgcagcg uucacauuca ccaagguccc agcugaaaca uugcauggaa     1560

caguuacagu ggaggugcag uaugcaggga cagauggacc cugcaagguc ccagcucaga     1620

uggcggugga caugcagacc cugaccccag ucggaaggcu gauaaccgcc aaccccguga     1680

uuacugaaag cacugagaau ucaaagauga uguuggagcu cgacccacca uuuggggauu     1740

cuuacauugu cauaggaguu ggggacaaga aaauuaccca ccacuggcau aggaguggua     1800

gcaccaucgg aaaagcauuu gaggccacug ugagaggugc caagagaaug gcaguucugg     1860

gggacacagc cugggacuuu ggaucagucg ggggcguguu uaacucauug ggcaagggca     1920

uccaccagau uuuuggagca gccuucaaau cacuguuugg aggaaugucc ugguucucac     1980

agauccucau aggcacgcug cugguguggu uggguuugaa cacaaagaau ggauccaucu     2040

cccucacaug cuuggcccug gggggaguga ugaucuuccu cuccacggcu gucucugcuu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  5447
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 2 wild type

<400>  5447
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cgucggccuc cugcugacca cagccauggc agccgagauc acuagacgug      120

ggagugcaua cuacauguac uuggacagga gcgaugcugg uaaggccauu ucuuuugcca      180

ccacauuggg ggugaacaaa ugccauguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc cccaugcuag acgagggagu ggagccagau gacgucgauu      300

gcuggugcaa cacgacaucg acuuggguug uguacggaac cugucaucau aaaaaaggug      360

aagcacgacg auccagaaga gccgugacgc uuccuucuca cuccacgagg aagcugcaaa      420

cgcgaucgca gacuuggcua gagucaagag aauacacaaa gcaccugauc aagguugaaa      480

auuggauauu caggaacccc ggguuugcgc uaguggcugu agcuauagcc uggcuccugg      540

gaagcucgac gagccaaaaa guuauauacu uggucaugau auuguugauu gccccggcau      600

acagcauuag gugcauagga guuagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacaugggg guugcgucac cgugauggca caggacaagc      720

caacaguuga uaucgaguug gucacgacaa cgguuagcaa cauggccgag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccguugucca acacaaggug      840

aagccuaccu ugacaagcag ucagacacuc aauaugucug caagagaaca uugguggaua      900

gagguugggg aaaugggugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacgugcuc caagaaaaug acaggcaaga gcauccagcc ggagaacuug gaguaccgga     1020

uaaugcuauc agugcaugga ucccagcaca gugggaugau ugugaaugac acaggacaug     1080

aaacugacga aaacagagca aaagucgagg ucacacccaa uucaccaaga gcagaagcaa     1140

ccuugggagg uuuuggaagc uugggacuug acugugaacc aaggacaggc cuugacuucu     1200

cagaucugua uuacuugacc augaacaaua agcauugguu ggugcacaag gagugguuuc     1260

augacauccc auuaccuugg caugcuggug cagacacugg aacuccacac uggaacaaca     1320

aagaggcauu gguggaguuc aaggacgccc acgccaagag gcaaacuguu gugguucugg     1380

ggagccaaga gggagcuguu cauacggccc ucgcuggagc uuuggaggcu gagauggaug     1440

gugcaaaggg aaggcuauuc ucuggccauu ugaaaugccg ccuaaaaaug gacaagcuua     1500

gguugaaggg ugugucauau ucccugugua ccgcagcguu cacauuuacc aaggucccag     1560

cugaaacauu gcauggaaca gucacagugg aggugcagua ugcagggaca gacggacccu     1620

gcaaaguccc agcccagaug gcgguggaca ugcagacccu gaccccaguu ggaaggcuga     1680

uaaccgccaa uccugugauc acugaaagua cugagaauuc aaagaugaug uuggagcucg     1740

acccaccauu uggggauucu uacauuguca uaggagucgg ggacaagaaa aucacccauc     1800

acuggcaucg gagugguagc accaucggaa aggcauuuga agccacugug agaggugcca     1860

agagaauggc agucuugggg gacacagccu gggacuuugg aucaguuggg gguguguuua     1920

auucauuggg uaaggguauu caccagaucu uuggagcagc uuucaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacacuguu ggugugguua gguuugaaca     2040

caaagaaugg aucuaucucc cucacaugcu uagcccuggg gggagugaug aucuuccuuu     2100

ccacggcugu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5448
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 2 wild type

<400>  5448
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cgucggccuc cugcugacca cagccauggc agccgagauc accagacgug      120

ggagugcaua cuacauguac uuggacagga gcgaugcugg uaaggccauu ucuuuugcua      180

ccacauuggg ggugaacaaa ugccauguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc cccaugcugg acgagggagu ggagccagau gacgucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaacau aaaaaaggug      360

aagcacgacg auccagaaga gccgugacgc uuccuucuca cuccacaagg aagcugcaaa      420

cgcgaucaca gacuuggcua gaaucaagag aauacacaaa gcaccugauc aagguugaga      480

auuggauauu caggaacccc ggguuugcgc uaguggcugu agcuauugcc uggcuccugg      540

gaagcucgac gagccaaaaa gucauauacu ugguaaugau auuguugauu gccccggcau      600

acagcaucag gugcauagga guuagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacaugggg guugcgucac cgugauggca caggacaagc      720

caacaguuga caucgaguug gucacgacaa cgguuagcaa cauggccgag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugucca acacaaggug      840

aagccuaccu ugacaagcag ucagacacuc aauaugucug caagagaaca uugguggaua      900

gagguugggg aaaugggugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacgugcuc caagaaaaug acaggcaaga gcauccagcc ggagaacuug gaguaccgga     1020

uaaugcuauc agugcaugga ucccagcaca gugggaugau ugugaaugac auaggacaug     1080

aaacugacga aaacagagca aaagucgagg ucacacccaa uucaccaaga gcagaagcaa     1140

ccuugggagg uuuuggaagc uugggacuug acugugaacc aaggacaggc cuugacuucu     1200

cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaag gagugguuuc     1260

augacauccc auuaccuugg caugcuggug cagacacugg aacuccacac uggaacaaca     1320

aagaggcauu gguggaguuu aaggacgccc acgccaagag gcaaacuguu gugguucugg     1380

ggagccaaga gggagcuguu cacacggccc ucgcuggagc uuuggaggcu gagauggaug     1440

gugcaaaggg aaggcuauuc ucuggccauu ugaaaugccg ccuaaaaaug gacaagcuua     1500

gguugaaggg ugugucauau ucccugugua ccgcagugug uaccgcagcg aaggucccag     1560

cugaaacauu gcauggaaca gucacagugg aggugcagua ugcagggaca gauggacccu     1620

gcaagguccc agcccagaug gcgguagaca ugcagacccu gaccccaguu ggaaggcuga     1680

uaaccgccaa cccugugauc acugaaagua cugagaauuc aaagaugaug uuggagcucg     1740

acccaccauu uggggauucu uacauuguca uaggagucgg ggacaagaaa aucacccauc     1800

acuggcaucg gagugguagc accaucggaa aggcauuuga agccacugug agaggugcca     1860

agagaauggc agucuugggg gacacagccu gggacuuugg aucaguuggg gguguguuua     1920

auucauuggg uaaggguauu caccagaucu uuggagcagc uuuuaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacacuguu ggugugguua ggucugaaca     2040

caaagaaugg aucuaucucc cucacaugcu uagcccuggg gggagugaug aucuuccuuu     2100

ccacggcugu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5449
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 2 wild type

<400>  5449
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cgucggccuc cugcugacca cagccauggc agccgagauc acuagacgug      120

ggagugcaua cuacauguac uuggacagga gcgaugcugg uaaggccauu ucuuuugcca      180

ccacauuggg ggugaacaaa ugccauguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc cccaugcuag acgagggagu ggagccagau gacgucgauu      300

gcuggugcaa cacgacaucg acuuggguug uguacggaac cugucaucau aaaaaaggug      360

aagcacgacg auccagaaga gccgugacgc uuccuucuca cuccacgagg aagcugcaaa      420

cgcgaucgca gacuuggcua gagucaagag aauacacaaa gcaccugauc aagguugaaa      480

auuggauauu caggaacccc ggguuugcgc uaguggcugu agcuauagcc uggcuccugg      540

gaagcucgac gagccaaaaa guuauauacu uggucaugau auuguugauu gccccggcau      600

acagcauuag gugcauagga guuagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacaugggg guugcgucac cgugauggca caggacaagc      720

caacaguuga uaucgaguug gucacgacaa cgguuagcaa cauggccgag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccguugucca acacaaggug      840

aagccuaccu ugacaagcag ucagacacuc aauaugucug caagagaaca uugguggaua      900

gagguugggg aaaugggugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacgugcuc caagaaaaug acaggcaaga gcauccagcc ggagaacuug gaguaccgga     1020

uaaugcuauc agugcaugga ucccagcaca gugggaugau ugugaaugac gaaaacagag     1080

caaaagucga ggucacaccc aauucaccaa gagcagaagc aaccuuggga gguuuuggaa     1140

gcuugggacu ugacugugaa ccaaggacag gccuugacuu cucagaucug uauuaccuga     1200

ccaugaacaa uaagcauugg uuggugcaca aggagugguu ucaugacauc ccauuaccuu     1260

ggcaugcugg ugcagacacu ggaacuccac acuggaacaa caaagaggca uugguggagu     1320

ucaaggacgc ccacgccaag aggcaaacug uugugguucu ggggagccaa gagggagcug     1380

uucauacggc ccucgcugga gcuuuggagg cugagaugga uggugcaaag ggaaggcuau     1440

ucucuggcca uuugaaaugc cgccuaaaaa uggacaagcu uagguugaag ggugugucau     1500

auucccugug uaccgcagcg uucacauuua ccaagguccc agcugaaaca uugcauggaa     1560

cagucacagu ggaggugcag uaugcaggga cagacggacc cugcaaaguc ccagcccaga     1620

uggcggugga caugcagacc cugaccccag uuggaaggcu gauaaccgcc aauccuguga     1680

ucacugaaag uacugagaau ucaaagauga uguuggagcu cgacccacca uuuggggauu     1740

cuuacauugu cauaggaguc ggggacaaga aaaucaccca ucacuggcau cggaguggua     1800

gcaccaucgg aaaggcauuu gaagccacug ugagaggugc caagagaaug gcagucuugg     1860

gggacacagc cugggacuuu ggaucaguug gggguguguu uaauucauug gguaagggua     1920

uucaccagau cuuuggagca gcuuucaaau cacuguuugg aggaaugucc ugguucucac     1980

agauccucau aggcacacug uugguguggu uagguuugaa cacaaagaau ggaucuaucu     2040

cccucacaug cuuagcccug gggggaguga ugaucuuccu uuccacggcu guuucugcuu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  5450
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 2 wild type

<400>  5450
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agcagagauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

agacacggcg aucuagaaga ucugugucgc uccguuauca cuauacaagg aaguugcaaa      420

cgcggucgca gacaugguua gaaucaagag aauacaagaa gcacuugauc auggucgaaa      480

acuggauauu caggaacccc ggguuugcca uaguguccgu ugccauuacc uggcugaugg      540

gaagcuugac gagccaaaaa gucauauacu uggucaugau aguguugauu gucccggcau      600

acaguaucag cugcauugga gucagcaaua gagacuuagu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag ggugcguuac cgagauggca caggacaagc      720

caacaguuga cauagaguug gucacgauga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucguua uccgacaugg cuucggccag ucguugccca acacaaggcg      840

aacccucccu cgacaagcaa ucagacacuc aaucuguaug caaaagaaca uuaggagaca      900

gagguugggg aaaugguugu gggauuuuug gcaaagggag cuuggugaca uguuccaagu      960

ucacguguug uaagaagaug cccgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuccc agugcauggc ucccagcaua gcgggaugau ugugaaugac auaggacaug     1080

aaacugacga aaacagagcg aaagucgagg ucacacccaa uucaccaaga gcagaagcaa     1140

ccuugggagg uuuuggaagc uugggacuug acugugaacc aaggacaggc cuugacuucu     1200

cagaucugua uuaucugacc augaacaaca agcauugguu ggugcacaag gagugguuuc     1260

augacauccc auuaccuugg caugcuggug cagacacugg aacuccacac uggaacaaca     1320

aagaggcauu gguggaguuc aaggacgccc acgccaagag gcaaacuguu gugguucugg     1380

ggagccaaga gggagcuguu cacacggccc ucgcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcuauuc ucuggccauu ugaaaugccg ccuaaaaaug gacaagcuua     1500

gguugaaggg ugugucauau ucccugugua cugcagcguu cacauuuacc aaggucccag     1560

cugaaacauu gcauggaaca guuacagugg aggugcaguc ugcagggaca gauggacccu     1620

gcaagguccc agcccagaug gcgguggaca ugcagacccu gaccccaguu ggaaggcuga     1680

uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug uuggagcuug     1740

acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa aucacccacc     1800

acuggcauag gagugguagc accaucggaa aggcauuuga agccacugug agaggcgcca     1860

agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg gguguguuca     1920

acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca cuguuuggag     1980

gaauguccug guucucacag auccucauag gcacgcugcu agugugguua gguuugaaca     2040

caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug aucuuccucu     2100

ccacggcugu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5451
<211>  2446
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 2 wild type

<400>  5451
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagug       60

ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu agacguggga      120

gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu uuuccaacca      180

cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug ugugaugcca      240

ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac gucgauuguu      300

ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa aaaggugaag      360

cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag cugcaaacgc      420

ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga gucgaaaauu      480

ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg cuuuugggaa      540

gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc ccggcauaca      600

gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca ggugggacuu      660

ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag gacaaaccga      720

cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua agauccuacu      780

gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca caaggugaag      840

ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua guggacagag      900

gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc gcuaaguuug      960

caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag uaccggauaa     1020

ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca ggacaugaaa     1080

cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc gaagccaccc     1140

ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu gacuuuucag     1200

auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag ugguuccacg     1260

acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg aacaacaaag     1320

aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug guucuaggga     1380

gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag auggauggug     1440

caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau aaacuuagau     1500

ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag aucccggcug     1560

aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau ggaccuugca     1620

agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg agguugauaa     1680

ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug gaacuugauc     1740

caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc acccaccacu     1800

ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga ggugccaaga     1860

gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc gcucucaacu     1920

cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug uuuggaggaa     1980

uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu cugaacacaa     2040

agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc uucuuaucca     2100

cagccgucuc ugcuugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2160

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2220

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2280

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2400

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2446


<210>  5452
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 2 wild type

<400>  5452
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aacuugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc caagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

ggagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5453
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 2 wild type

<400>  5453
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

acuggauguu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguccc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5454
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 2 wild type

<400>  5454
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa caugacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucggacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugacc ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguc cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa cccuguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gagugguagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaacgg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5455
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 2 wild type

<400>  5455
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcggagguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc ucccuuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cgcaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauauguuug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugcu cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu ggcucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagccaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacagu ggacgggaca gucacagugg agggacagua cggagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcagacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauua uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug ggacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucagguuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5456
<211>  2440
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 2 wild type

<400>  5456
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggag       60

cugaugccag caucggaauc gucagccucc ugcugacuac agucauggca gcagagauca      120

cuagacgcgg gagugcauac uacauguacu uggacaggag cgaugcuggu aaggccauuu      180

cuuucguuac cacacugggg gugaacaaau gccaugugca gaucauggac cucgggcaua      240

ugugugacgc caccaugagu uaugagugcc ccaugcugga cgagggagug gagccagaug      300

acgucgauug cuggugcaac acgacaucaa cuuggguugu guacggaacc ugucaucaua      360

aaaaagguga agcacgacga uccagaagag ccgugacgcu uccuucucac ucuacaagga      420

aguugcaaac gcgaucgcag acuuggcuag aaucaagaga auacacaaag caccugauca      480

agguugagaa uuggauauuc aggaaccccg gguuugcgcu aguggcugua gcuauugccu      540

ggcuccuggg aagcucgacg agccaaaaag ucauauacuu ggucaugaua uuguugauug      600

ccccggcaua caguaucagg ugcauaggag uuagcaauag agacuucgug gagggcaugu      660

caggugggac cuggguugau guugucuugg aacauggagg uugugucacc gugauggcac      720

aggacaagcc aacaguugac auagaguugg ucacgacaac gguuagcaac auggccgagg      780

ugagauccua cugcuacgag gcaucaauau cggacauggc uucggacagu cgcugcccaa      840

cacaagguga agccuaccuu gacaagcagu cagacacuca auaugucugu aaaagaacau      900

ugguggacag agguugggga aaugggugug gacuuuuugg caaggggagc uuggugacgu      960

gugccaaguu uacaugcucc aagaaaauga cagggaagag cauccagccg gagaacuugg     1020

aguaccggau aaugcuauca gugcauggau cccagcacag ugggaugauu gugaaugacg     1080

aaaacagagc aaaagucgag guuacaccca auucaccaag agcagaagca accuugggag     1140

guuuuggaag ccugggacuu gauugugaac caaggacagg ccuugacuuu ucagaucugu     1200

auuaccugac caugaacaau aagcauuggu uggugcacaa agagugguuu caugacaucc     1260

cauuaccuug gcauucuggu gcagacacug aaacuccaca cuggaacaac aaagaggcac     1320

ugguggaguu caaggacgcc cacgccaaga ggcaaacugu ugugguucug gggagccaag     1380

aaggagccgu ucacacggcu cucgcuggag cucuggaggc ugagauggau ggugcgaagg     1440

gaaggcuauc cucaggccau uugaaaugcc gccuaaaaau ggacaagcuu agguugaagg     1500

gugugucaua uucccugugu accgcagcgu ucacauucac caagguucca gcugaaacau     1560

ugcauggaac agucacagug gaggugcagu augcagggag ggauggaccc ugcaaggucc     1620

cagcccagau ggcgguggac augcagaccc ugaccccagu uggaaggcug auaacggcua     1680

acccugugau cacugaaagc acugagaauu caaagaugau guuggagcuc gacccaccau     1740

uuggggauuc uuacauuguc auaggagucg gggacaagaa aaucacccau cacuggcauc     1800

ggagugguag caucaucgga aaggcauuug aagccacugu gagaggcgcc aagagaaugg     1860

cagucuuggg agacacagcc ugggacuuug gaucaguugg ggguguguuu aacucauugg     1920

gcaaggguau ucaccagauc uuuggagcag cuuucaaauc acuguucgga ggaauguccu     1980

gguucucaca gauccucaua ggcacacugu uggugugguu gggucugaac acaaagaaug     2040

gaucuaucuc ccucacaugc uuggccuugg gaggagugau gaucuuccuu uccacggcug     2100

uuucugcuug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2160

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2220

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2280

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2400

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2440


<210>  5457
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 2 wild type

<400>  5457
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

cagacaccag caucggaauc auuggccucc ugcugacuac agccauggca gcagagauca      120

cuagacgcgg gagugcauac uacauguacu uggauaggag cgaugccggg aaggccauuu      180

cguuugcuac cacaauggga gugaacaagu gccacguaca gaucauggac cucgggcaca      240

ugugugacgc caccaugagu uaugagugcc cuaugcugga ugagggagug gaaccagaug      300

augucgauug cuggugcaac acgacaucaa cuuggguugu guacggaacc ugucaucaca      360

aaaaagguga ggcacggccc ucaagaagag cugucacgcu uccuucccau uccacaagaa      420

aguuacagac acggucacaa accuggcuug aaucacggga guacacgaag caccugauca      480

aaguugagaa cuggauacuc cggaauccag gguucacagu ugcugcucua gcaauggccu      540

ggcucuuggg aaauuccacg agccagcgag ugaucuaucu ggugaugauc cuguugauug      600

ccccggcaua caguaucagg ugcauuggag ucagcaauag agacuucgug gagggcaugu      660

caggugggac cuggguugau guugucuugg aacauggagg cugcguuacc gugauggcac      720

aggacaagcc aacagucgac auagaguugg ucacgacgac gguuaguaac auggccgagg      780

uaagauccua uugcuacgag gcaucgauau cggacauggc uucggacagu cguugcccaa      840

cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc aaaagaacau      900

uaguggacag agguugggga aacgguugug gacuuuuugg caaagggagc uuggugacau      960

gugccaaguu uacguguucu aagaagauga ccgggaagag cauucaaccg gagaauuugg     1020

aguacagaau uaugcuguca gugcacggcu cccaacacag cgggaugauu gugaaugaca     1080

uaggacauga gaccgaugaa aacagagcaa aaguggaggu cacaccaaac ucaccaagag     1140

cugaagcgac ccuagggggc uucgggaguu ugggacucga uugugagcca aggaccgguu     1200

uagauuucuc cgaccuguac uauuuaacca ugaacaacaa gcauugguug gugcacaagg     1260

agugguucca cgacauucca uugccauggc acgcuggagc ggacacaggg acuccccauu     1320

ggaacaacaa agaggcauug guugaguuca aggaugccca cgccaaaaga cagacuguug     1380

ucguguuggg aagucaggag ggugccgucc acacagcccu ggcaggagcu uuggaagcgg     1440

agauggaugg agccaaagga aggcuguucu caggucauuu gaagugccgg auaaagaugg     1500

acaagcuuag guugaagggu gucucauacu cauugugcac ugccgccuuc acauucucaa     1560

agguuccggc ugaaacgcug caugggacag ucacaguuga ggugcaguau gcgggcagag     1620

auggaccuug caaggugcca gcccaaaugg caguggauau gcagacucuu accccagugg     1680

gaagguugau aacggcaaau cccaucauaa cugagagcac ugaaaacucg agaaugaugu     1740

uggagcugga cccacccuuu ggagacucuu acauugucau ugggguugga gauaagaaaa     1800

uuacccauca uuggcacaga aguggaagca cgauugggaa ggcauuugag gccacuguga     1860

gaggugcaaa aaggauggca guccugggag auaccgccug ggauuuuggu ucuguuggag     1920

gcguguucaa uucucugggc aagggaguuc aucaggucuu cggagcagcc uuuaaauccu     1980

uauuuggcgg aauguccugg uuuucacaaa uucuuauugg cacacuuuua gugugguugg     2040

gcuugaauac aaaaaaugga uccauuuccc uugcaugucu ggcgcuugga ggggcgauga     2100

ucuuucucuc cacggccguc ucggcaugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  5458
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 2 wild type

<400>  5458
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5459
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 2 wild type

<400>  5459
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5460
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 2 wild type

<400>  5460
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      240

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      420

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      480

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc      540

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg      600

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga agcuuaggac      660

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca      720

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug      780

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uucaaggaug      840

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg      900

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc      960

auuugaaaug ccgccuaaaa auggacaagc uuagauugaa gggcguguca uauuccuugu     1020

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1080

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1140

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1200

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1260

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1320

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1380

ccugggacuu cggaucauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  5461
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 2 wild type

<400>  5461
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguuggucc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5462
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 2 wild type

<400>  5462
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      240

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      420

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      480

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc      540

auggcuccca gcauagcggg augauuguca augauacagg auaugaaacu gacgaaaaua      600

gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug ggaggcuuug      660

gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau cuguauuacc      720

ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac aucccauugc      780

cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag gcauugguag      840

aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc caggaaggag      900

ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca aagggaaagc      960

uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug aagggcgugu     1020

cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa acacugcaug     1080

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag aucccagucc     1140

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaaccccg     1200

ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca ccauuugggg     1260

auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg cauaggagug     1320

guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga auggcagucc     1380

ugggggauac agccugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5463
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 2 wild type

<400>  5463
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca guugacauag      240

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      420

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      480

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc      540

auggcuccca gcauagcggg augacuguca augauauagg auaugaaacu gacgaaaaua      600

gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug ggaggcuuug      660

gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau cuguauuacc      720

ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac aucccauugc      780

cuuggcaugc uggggcagac acuggaacuc cacacuggaa caacaaagag gcauugguag      840

aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc caggaaggag      900

ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca aagggaaagc      960

uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug aagggcgugu     1020

cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa acacugcaug     1080

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag aucccagucc     1140

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaaccccg     1200

ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca ccauuugggg     1260

auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg cauaggagug     1320

guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga auggcagucc     1380

ugggggauac agccugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5464
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 2 wild type

<400>  5464
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uaggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugacguug      180

uccuggaaca uggaggcugc gucaccguga uggcacagga caagccaaca guugacauag      240

agcuggucac aacaacgguu aguaacaugg ccgaggugag auccuauugu uacgaggcau      300

caauaucgga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau guuugcaaaa gaacauuggu ggacagaggu uggggaaaug      420

gguguggacu cuuuggcaaa gggaguuugg ugacaugugc uaaguucacg ugcuccaaga      480

agaugacugg gaagagcauu cagccggaga accuggagua ucggauaaug cuaucagugc      540

auggcuccca gcacaguggg augauuguua augaugaaaa cagagcgaag gucgagguua      600

cgcccaauuc accaagagca gaagcaaccc ugggaggcuu uggaagcuua ggacuugauu      660

gugaaccaag gacaggccuu gacuuuucag aucuguauua ccuaaccaug aauaacaagc      720

auugguuggu gcacaaagag ugguuucaug acaucccauu gcccuggcau gcuggggcag      780

acacuggaac uccacauugg aacaacaagg aggcauuagu ggaauucaag gacgcccacg      840

ccaagaggca aaccgucgug guuuugggga gccaggaagg agccguccac acggcucuug      900

cuggagcucu agaggcugag auggauggug caaagggaag gcuauucucu ggccacuuga      960

aaugucgcuu aaaaauggac aagcuuagau ugaagggcgu gucauauucc uugugcaccg     1020

cggcauucac auucaccaag gucccggcug aaacacuaca uggaacaguc acaguggagg     1080

ugcaguaugc agggacagau ggacccugca aggucccagc ccagauggcg guggacaugc     1140

agaccuugac cccagucgga aggcugauaa ccgccaaccc cgugauuacu gaaagcacug     1200

agaauucaaa gaugauguug gagcucgacc caccauuugg ggauucuuac auugucauag     1260

gaguugggga uaagaaaauc acccaucacu ggcauaggag uggcagcacc auuggaaaag     1320

cauuugaagc cacugugaga ggcgcuaaga gaauggcagu ccugggggac acagcuuggg     1380

acuuuggauc augaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  5465
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 2 wild type

<400>  5465
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      240

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      420

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      480

agaugaccgg gaagagcauu caaccggaaa aucuggagua ccggauaaug cuaucagugc      540

auggcuccca gcauagcggg augauuggau augaaacuga cgaaaauaga gcgaaagucg      600

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga agcuuaggac      660

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca      720

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug      780

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uucaaggaug      840

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg      900

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaagcug uucucuggcc      960

auuugaaaug ccgccuaaaa auggacaagc uuagauugaa gggcguguca uauuccuugu     1020

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1080

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1140

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1200

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1260

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1320

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1380

ccugggacuu cggaucauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  5466
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 2 wild type

<400>  5466
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc cucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucu gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5467
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 2 wild type

<400>  5467
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagca       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5468
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 2 wild type

<400>  5468
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg uacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5469
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 2 wild type

<400>  5469
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaagc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5470
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 2 wild type

<400>  5470
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caauaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacacgcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5471
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 2 wild type

<400>  5471
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagauaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacuggugg      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5472
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 2 wild type

<400>  5472
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacuccaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaggaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5473
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 2 wild type

<400>  5473
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga caugguuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuucucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5474
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 2 wild type

<400>  5474
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauauagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccaccuug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcauugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5475
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 2 wild type

<400>  5475
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auauuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acucagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga aucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5476
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 2 wild type

<400>  5476
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccu ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug uugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagccc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5477
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 2 wild type

<400>  5477
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggggguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagcuuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggacu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5478
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 2 wild type

<400>  5478
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guucuagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5479
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 2 wild type

<400>  5479
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagcuuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga agcacaugcu aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5480
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 2 wild type

<400>  5480
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccgcccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5481
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 2 wild type

<400>  5481
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuuc aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcaa      300

caauaucaga cauagcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugccguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5482
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 2 wild type

<400>  5482
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucuccaagau cccggcugaa acacugcacg     1080

ggacagucac agaggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccgg     1200

uaaucacuga aagcacagag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5483
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 2 wild type

<400>  5483
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucu gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaagu ugagauaacg cccaauucac cgagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaguua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5484
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 2 wild type

<400>  5484
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggaaucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaguau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaauccuagg acuggauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5485
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 2 wild type

<400>  5485
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugua aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaaa ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

uaggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5486
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 2 wild type

<400>  5486
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuggu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcau ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag gggcagaugg acccugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gccaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5487
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 2 wild type

<400>  5487
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag uaauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa caacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacac uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aggcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5488
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 2 wild type

<400>  5488
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccgcaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau guuugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augcucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguuggcuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagcc accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acaguggacg     1080

ggacagucac aguggaggga caguacggag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcag acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5489
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 2 wild type

<400>  5489
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu guuaccguaa uggcacagga caaaccggcu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuauugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcagucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagcuga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcaugc uggggcagac accggaacuc cacauuggaa caacaaagaa gcauugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg acccugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag gcugauaacc gcuaacccug     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaucacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5490
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 2 wild type

<400>  5490
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauauagc aucaggugca      120

uaggagucaa caauagggac uuuguggaag guaugucagg ugggacuugg guugaugucg      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cagagguaag auccuacugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc caaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccgaauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggagguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga ugcacaugcc aaaaggcaaa cugucguggu ccuagggagu caagagggag      900

caguucacac ggcucuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagacug aagggcgugu     1020

cauacucccu guguaccgca gcguucacau ucacuaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5491
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 2 wild type

<400>  5491
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auauuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggaggcugu guuaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguu agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucgga uauggcuucg gacagccgcu gcccaacaca aggugaggcc uaccuugaca      360

agcagucaga cacucaauau gucugcaaaa gaacguuagu ggauagaggc uggggaaaug      420

gauguggacu uuuuggaaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga accuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacc      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcaugc uggggcagac acuggaacuc cacauuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acucagauug aagggugugu     1020

cauacuccuu gugugcugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1140

agauggcggu ggauaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1200

uaaucacuga aagcaccgag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5492
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 2 wild type

<400>  5492
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugu ugauugcccc ggcauacagu aucaggugca      120

uaggagucag caauagggau uuuguggaag guaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggagguugu guuaccguaa uggcacagga caagccaacu guugauauag      240

aguuggucac aacaacgguu agcaacaugg cggagguaag auccuacugc uacgaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcagucaga cacucaauau guuugcaaaa gaacguuagu ggacagaggu uggggaaaug      420

gauguggacu cuuuggcaaa gggagccugg ugacaugcgc caaguuugca ugcuccaaga      480

aaaugacugg gaagagcauc cagccagaga accuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augauuguua augacauagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggagguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcau ugguuggugc acaaggagug guuccaugac auuccacuac      780

cuuggcaugc uggggcagac accggaacuc cacauuggaa caacaaagaa gcauugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

ccguucacac ggcucuugcu ggagcccugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgcuuga aaauggacaa acuuagauug aagggcgugu     1020

cauacuccuu auguaccgcg gcguucacau ucaccaagau cccggcugaa acgcugcaug     1080

ggacagucac aguggaggua caguaugcag ggacagaugg acccugcaag guuccagcuc     1140

agauggcggu ggauaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1200

ugaucacuga aagcacugag aauucaaaga ugauguugga acuugaccca ccauuugggg     1260

auucuuacau ugucauagga guuggggaua agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau cggaaaagca uuugaagcca cugugagagg cgccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5493
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 2 wild type

<400>  5493
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugu ugauugcccc ggcauacagu aucaggugca      120

uaggagucag caauagggau uuuguggaag guaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggagguugu guuaccguaa uggcacagga caagccaacu guugauauag      240

aguuggucac aacaacgguu agcaacaugg cggagguaag auccuacugc uacgaggcau      300

caauaucgga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcagucaga cacucaauau guuugcaaaa gaacguuagu ggacagaggu uggggaaaug      420

gauguggacu cuuuggcaaa gggagccugg ugacaugcgc caaguuugca ugcuccaaga      480

aaaugacugg gaagagcauc cagccagaga accuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augauuguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggagguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcau ugguuggugc acaaggagug guuccaugac auuccacuac      780

cuuggcaugc uggggcagac accggaacuc cacauuggaa caacaaagaa gcauugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

ccguucacac ggcucuugcu ggagcccugg aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgcuuga aaauggacaa acuuagauug aagggcgugu     1020

cauacuccuu auguaccgcg gcguucacau ucaccaagau cccggcugaa acgcugcaug     1080

ggacagucac aguggaggua caguaugcag ggacagaugg acccugcaag guuccagcuc     1140

agauggcggu ggauaugcaa acucugaccc caguugggag guugauaacc gcuaacccug     1200

ugaucacuga aagcacugag aauucaaaga ugauguugga acuugaccca ccauuugggg     1260

auucuuacau ugucauagga guuggggaua agaagaucac ccaccacugg uacaggagug     1320

gcagcaccau cggaaaagca uuugaagcca cugugagagg cgccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5494
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 2 wild type

<400>  5494
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      120

uaggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugacguug      180

uccuggaaca uggaggcugc gucaccguga uggcacagga caagccaaca guugacauag      240

agcuggucac aacaacgguu aguaacaugg ccgaggugag auccuauugu uacgaggcau      300

caauaucgga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau guuugcaaaa gaacauuggu ggacagaggu uggggaaaug      420

gguguggacu cuuuggcaaa gggaguuugg ugacaugugc uaaguucacg ugcuccaaga      480

agaugacugg gaagagcauu cagccggaga accuggagua ucggauaaug cuaucagugc      540

auggcuccca gcacaguggg augauuguua augacacagg acaugaaacu gaugaaaaca      600

gagcgaaggu cgagguuacg cccaauucac caagagcaga agcaacccug ggaggcuuug      660

gaagcuuagg acuugauugu gaaccaagga caggccuuga cuuuucagau cuguauuacc      720

uaaccaugaa uaacaagcau ugguuggugc acaaagagug guuucaugac aucccauugc      780

ccuggcaugc uggggcagac acuggaacuc cacauuggaa caacaaggag gcauuagugg      840

aauucaagga cgcccacgcc aagaggcaaa ccgucguggu uuuggggagc caggaaggag      900

ccguccacac ggcucuugcu ggagcucuag aggcugagau ggauggugca aagggaaggc      960

uauucucugg ccacuugaaa ugucgcuuaa aaauggacaa gcuuagauug aagggcgugu     1020

cauauuccuu gugcaccgcg gcauucacau ucaccaaggu cccggcugaa acacuacaug     1080

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag gucccagccc     1140

agauggcggu ggacaugcag accuugaccc cagucggaag gcugauaacc gccaaccccg     1200

ugauuacuga aagcacugag aauucaaaga ugauguugga gcucgaccca ccauuugggg     1260

auucuuacau ugucauagga guuggggaua agaaaaucac ccaucacugg cauaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg cgcuaagaga auggcagucc     1380

ugggggacac agcuugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5495
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 2 wild type

<400>  5495
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguu augauacugc ugauugcccc ggcauacagc aucaggugca      120

uuggagucag caauagagac uucguggaag gcaugucagg ugggaccugg guugaugucg      180

ucuuggaaca uggagguugc gucaccguua uggcacagga caagccaaca guugacauag      240

aguuggucac gacaacgguu aguaacaugg ccgagguaag auccuacugu uaugaggcau      300

caauaucaga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaua      360

agcaaucaga cacccaauau gugugcaaaa gaacauuggu ggacagaggu uggggaaaug      420

gguguggacu uuuuggcaaa gggagccugg ugacaugugc caaguucacg uguuccaaga      480

agaugacugg gaagagcauu cagccggaga aucuggagua ucgaauaaug cuaucagugc      540

auggcuccca gcacagcggg augauuguua augacauagg acaugaaacu gacgaaaaca      600

gagcgaaagu cgaggucacg cccaauucac caagagcaga agcaaccuug ggaggcuuug      660

gaagcuuagg acuugauugu gaaccaagga caggccugga cuuuucagau uuguacuacu      720

ugaccaugaa caacaagcac ugguuggugc acaaagagug guuucaugac aucccauugc      780

cuuggcaugc uggggcagau accggaacuc cacacuggaa caacaaggag gcauugguag      840

aguucaaaga ugcccacgcc aagaggcaaa ccgucguugu ucuagggagc caggaaggag      900

ccguucacac ggcucuugcu ggagcucugg aggcugagau ggauggugca aaaggaaggc      960

uguucuccgg ccauuugaaa ugccgcuuaa aaauggacaa gcuuagauug aagggcgugu     1020

cauauuccuu gugcaccgca gcguucacau uuaccaaggu cccagcugaa acauugcaug     1080

gaacaguuac aguggaggug caguaugcag ggacagaugg acccugcaag gucccagccc     1140

agauggcggu ggacaugcag acccugaccc cagucgggag gcugauaacc gccaacccug     1200

ugauuacuga aagcacugag aauucaaaga ugauguugga gcucgaccca ccauuugggg     1260

auucuuacau ugucauagga guuggggaca agaaaaucac ccaucacugg cauaggagug     1320

guagcaccau cggaaaagca uuugaggcca cugugagagg ugccaagaga auggcaguuc     1380

ugggggauac agccugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5496
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 2 wild type

<400>  5496
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuuggua augauauugu ugauugcccc ggcauacagc aucaggugca      120

uaggaguuag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggggguugc gucaccguga uggcacagga caagccaaca guugacaucg      240

aguuggucac gacaacgguu agcaacaugg ccgagguaag auccuacugc uaugaggcau      300

caauaucgga cauggcuucg gacagccgcu guccaacaca aggugaagcc uaccuugaca      360

agcagucaga cacucaauau gucugcaaga gaacauuggu ggauagaggu uggggaaaug      420

gguguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg ugcuccaaga      480

aaaugacagg caagagcauc cagccggaga acuuggagua ccggauaaug cuaucagugc      540

auggauccca gcacaguggg augauuguga augacauagg acaugaaacu gacgaaaaca      600

gagcaaaagu cgaggucaca cccaauucac caagagcaga agcaaccuug ggagguuuug      660

gaagcuuggg acuugacugu gaaccaagga caggccuuga cuucucagau cuguauuacc      720

ugaccaugaa caauaagcau ugguuggugc acaaggagug guuucaugac aucccauuac      780

cuuggcaugc uggugcagac acuggaacuc cacacuggaa caacaaagag gcauuggugg      840

aguuuaagga cgcccacgcc aagaggcaaa cuguuguggu ucuggggagc caagagggag      900

cuguucacac ggcccucgcu ggagcuuugg aggcugagau ggauggugca aagggaaggc      960

uauucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagguug aagggugugu     1020

cauauucccu guguaccgca guguguaccg cagcgaaggu cccagcugaa acauugcaug     1080

gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag gucccagccc     1140

agauggcggu agacaugcag acccugaccc caguuggaag gcugauaacc gccaacccug     1200

ugaucacuga aaguacugag aauucaaaga ugauguugga gcucgaccca ccauuugggg     1260

auucuuacau ugucauagga gucggggaca agaaaaucac ccaucacugg caucggagug     1320

guagcaccau cggaaaggca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggggacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5497
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 2 wild type

<400>  5497
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauauugu ugauugcccc ggcauacagu aucaggugca      120

uaggaguuag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggagguugu gucaccguga uggcacagga caagccaaca guugacauag      240

aguuggucac gacaacgguu agcaacaugg ccgaggugag auccuacugc uacgaggcau      300

caauaucgga cauggcuucg gacagucgcu gcccaacaca aggugaagcc uaccuugaca      360

agcagucaga cacucaauau gucuguaaaa gaacauuggu ggacagaggu uggggaaaug      420

gguguggacu uuuuggcaag gggagcuugg ugacgugugc caaguuuaca ugcuccaaga      480

aaaugacagg gaagagcauc cagccggaga acuuggagua ccggauaaug cuaucagugc      540

auggauccca gcacaguggg augauuguga augacgaaaa cagagcaaaa gucgagguua      600

cacccaauuc accaagagca gaagcaaccu ugggagguuu uggaagccug ggacuugauu      660

gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug aacaauaagc      720

auugguuggu gcacaaagag ugguuucaug acaucccauu accuuggcau ucuggugcag      780

acacugaaac uccacacugg aacaacaaag aggcacuggu ggaguucaag gacgcccacg      840

ccaagaggca aacuguugug guucugggga gccaagaagg agccguucac acggcucucg      900

cuggagcucu ggaggcugag auggauggug cgaagggaag gcuauccuca ggccauuuga      960

aaugccgccu aaaaauggac aagcuuaggu ugaagggugu gucauauucc cuguguaccg     1020

cagcguucac auucaccaag guuccagcug aaacauugca uggaacaguc acaguggagg     1080

ugcaguaugc agggagggau ggacccugca aggucccagc ccagauggcg guggacaugc     1140

agacccugac cccaguugga aggcugauaa cggcuaaccc ugugaucacu gaaagcacug     1200

agaauucaaa gaugauguug gagcucgacc caccauuugg ggauucuuac auugucauag     1260

gagucgggga caagaaaauc acccaucacu ggcaucggag ugguagcauc aucggaaagg     1320

cauuugaagc cacugugaga ggcgccaaga gaauggcagu cuugggagac acagccuggg     1380

acuuuggauc augaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  5498
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 2 wild type

<400>  5498
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auacuugguc augauagugu ugauuguccc ggcauacagu aucagcugca      120

uuggagucag caauagagac uuaguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggagggugc guuaccgaga uggcacagga caagccaaca guugacauag      240

aguuggucac gaugacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cguuauccga cauggcuucg gccagucguu gcccaacaca aggcgaaccc ucccucgaca      360

agcaaucaga cacucaaucu guaugcaaaa gaacauuagg agacagaggu uggggaaaug      420

guugugggau uuuuggcaaa gggagcuugg ugacauguuc caaguucacg uguuguaaga      480

agaugcccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cucccagugc      540

auggcuccca gcauagcggg augauuguga augacauagg acaugaaacu gacgaaaaca      600

gagcgaaagu cgaggucaca cccaauucac caagagcaga agcaaccuug ggagguuuug      660

gaagcuuggg acuugacugu gaaccaagga caggccuuga cuucucagau cuguauuauc      720

ugaccaugaa caacaagcau ugguuggugc acaaggagug guuucaugac aucccauuac      780

cuuggcaugc uggugcagac acuggaacuc cacacuggaa caacaaagag gcauuggugg      840

aguucaagga cgcccacgcc aagaggcaaa cuguuguggu ucuggggagc caagagggag      900

cuguucacac ggcccucgcu ggagcucugg aggcugagau ggauggugca aagggaaggc      960

uauucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagguug aagggugugu     1020

cauauucccu guguacugca gcguucacau uuaccaaggu cccagcugaa acauugcaug     1080

gaacaguuac aguggaggug cagucugcag ggacagaugg acccugcaag gucccagccc     1140

agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc gccaaccccg     1200

ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca ccauuugggg     1260

auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg cauaggagug     1320

guagcaccau cggaaaggca uuugaagcca cugugagagg cgccaagaga auggcagucc     1380

ugggggauac agccugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5499
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 2 wild type

<400>  5499
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      120

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      180

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      240

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      300

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      420

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      480

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      540

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      600

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      660

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      720

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac      780

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag      840

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag      900

caguucacac ggcccuugcu ggagcucuag aggcugagau ggauggugca aagggaaggc      960

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1020

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1080

ggacagucac aguggaggua caguacgcag ggacugaugg accuugcaag guuccagcuc     1140

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1200

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1260

acuccuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1320

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1380

ugggagacac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5500
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 2 wild type

<400>  5500
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agcgagugau cuaucuggug augauccugu ugauugcccc ggcauacagu aucaggugca      120

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      180

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      240

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      300

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      360

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      420

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      480

agaugaccgg gaagagcauu caaccggaga auuuggagua cagaauuaug cugucagugc      540

acggcuccca acacagcggg augauuguga augacauagg acaugagacc gaugaaaaca      600

gagcaaaagu ggaggucaca ccaaacucac caagagcuga agcgacccua gggggcuucg      660

ggaguuuggg acucgauugu gagccaagga ccgguuuaga uuucuccgac cuguacuauu      720

uaaccaugaa caacaagcau ugguuggugc acaaggagug guuccacgac auuccauugc      780

cauggcacgc uggagcggac acagggacuc cccauuggaa caacaaagag gcauugguug      840

aguucaagga ugcccacgcc aaaagacaga cuguugucgu guugggaagu caggagggug      900

ccguccacac agcccuggca ggagcuuugg aagcggagau ggauggagcc aaaggaaggc      960

uguucucagg ucauuugaag ugccggauaa agauggacaa gcuuagguug aagggugucu     1020

cauacucauu gugcacugcc gccuucacau ucucaaaggu uccggcugaa acgcugcaug     1080

ggacagucac aguugaggug caguaugcgg gcagagaugg accuugcaag gugccagccc     1140

aaauggcagu ggauaugcag acucuuaccc cagugggaag guugauaacg gcaaauccca     1200

ucauaacuga gagcacugaa aacucgagaa ugauguugga gcuggaccca cccuuuggag     1260

acucuuacau ugucauuggg guuggagaua agaaaauuac ccaucauugg cacagaagug     1320

gaagcacgau ugggaaggca uuugaggcca cugugagagg ugcaaaaagg auggcagucc     1380

ugggagauac cgccugggau uuugguucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5501
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5050/R5051 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 2 GC

<400>  5501
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcucc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      720

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      780

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1680

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1740

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc gcggugagcg     2160

ccgacguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5502
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 GC

<400>  5502
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcucc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacacgug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

cgagcaccug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cgcgcaagcu gcagacccgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccacgagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg guggacgucg      720

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      780

agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac cuguacuacc     1260

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg     1380

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc caggaggggg     1440

ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acgcuccacg     1620

gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1680

agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg     1740

ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1920

ugggggacac ggcgugggac uucggcagcg ucggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacc cugcugaugu ggcucgggcu gaacgccaag aacggcucca     2100

ucagccugau gugccucgcg cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5503
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 GC

<400>  5503
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag cggaagcgcc ggggcgccga caccagcauc gggaucaucg      120

gccugcugcu cacgaccgcc auggcggccg agaucacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcaagg ccaucuccuu cgcgaccacg cugggcguga      240

acaagugcca cgugcagauc auggaccucg ggcacaugug cgacgccacc augagcuacg      300

agugcccgau gcuggacgag ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccacgug gguggucuac gggaccugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu gacccugccc ucccacagca cccggaagcu ccagacgcgc ucccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugaucaaggu ggagaacugg aucuuccgga      540

accccggcuu cgcccucguc gcgguggcca ucgccuggcu gcuggggucc agcaccuccc      600

agaaggugau cuaccucguc augauccugc ugaucgcccc ggccuacagc auccgcugca      660

ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg gucgacgugg      720

ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc uacgaggcca      840

gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga     1020

agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg gccaaggugg     1140

aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc agccucgggc     1200

uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug acgaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg uggcacgcgg     1320

gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg guccacacgg     1440

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug uucuccgggc     1500

accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc uacucccucu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc acggucaccg     1620

uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag auggcggugg     1680

acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc aucaccgaga     1740

gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac agcuacaucg     1800

ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1860

ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc ggggacaccg     1920

ccugggacuu cggcagcgug ggcggcgucu ucaacucccu ggggaagggg auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc cagauccuca     2040

ucgggacgcu gcuggugugg cucggccuga acaccaagaa cggcuccauc agccugaccu     2100

gccucgcgcu gggcggcgug augaucuucc uguccaccgc cgucagcgcc gacguggggu     2160

gcuccgugga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5504
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 2 GC

<400>  5504
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcucc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacgucg      720

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      780

agcucgugac cacgaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg     1380

aguucaagga cgcgcacgcc aagcgccaga ccguggucgu gcugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugacgc ccgucgggcg ccugaucacc gcgaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gucggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ucggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gcggugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5505
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5052/R5053 ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA 
       Design 2 GC

<400>  5505
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5506
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 2 GC

<400>  5506
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac acgugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccacgagc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacgcgc aagcugcaga      420

cccggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac gagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugacgacca ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacacgc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac acgggccacg     1080

agaccgacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggacgggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagacggug gucgugcugg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacgcu ccacggcacc gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacgcu gacccccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca cggagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacacggcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaacg     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcgcuggg cggcgugcug aucuuccucu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5507
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 2 GC

<400>  5507
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucgggau caucggccug cugcucacga ccgccauggc ggccgagauc acccgccggg      120

gguccgccua cuacauguac cuggaccgca gcgacgccgg caaggccauc uccuucgcga      180

ccacgcuggg cgugaacaag ugccacgugc agaucaugga ccucgggcac augugcgacg      240

ccaccaugag cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccucc acgugggugg ucuacgggac cugccaccac aagaagggcg      360

aggcccggcg cagccggcgc gccgugaccc ugcccuccca cagcacccgg aagcuccaga      420

cgcgcuccca gaccuggcug gagagccgcg aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu ccggaacccc ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg      540

gguccagcac cucccagaag gugaucuacc ucgucaugau ccugcugauc gccccggccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggga      660

cgugggucga cguggugcuc gagcacggcg gcugcgucac cgugauggcg caggacaagc      720

ccaccgugga caucgagcug gucaccacga ccguguccaa cauggccgag guccgcagcu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagcgcacg cucguggacc      900

ggggcugggg gaacggcugc gggcuguucg gcaagggcuc ccuggucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcugag cgugcacggc ucccagcaca gcgggaugau cggcuacgag acggacgagg     1080

accgggccaa gguggagguc accccgaacu ccccgcgcgc cgaggccacc cugggcgggu     1140

ucggcagccu cgggcuggac ugcgagcccc ggaccggccu ggacuucucc gaccucuacu     1200

accugacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ucccguggca cgcgggggcc gacaccggca ccccgcacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagcgcc agaccgucgu ggugcugggc agccaggagg     1380

gggcggucca cacggcccug gccggcgccc ucgaggccga gauggacggg gcgaagggcc     1440

ggcuguucuc cgggcaccug aagugccgcc ucaagaugga caagcugcgg cugaagggcg     1500

ugagcuacuc ccucugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcacggu caccguggag gugcaguacg ccgggaccga cggccccugc aagaucccgg     1620

uccagauggc gguggacaug cagacccuga cgcccguggg gcgccucauc accgccaacc     1680

ccgucaucac cgagagcacc gagaacucca agaugaugcu ggagcuggac ccgccguucg     1740

gcgacagcua caucgugauc ggggugggcg acaagaagau cacgcaccac uggcaccgga     1800

gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgcgaag cggauggccg     1860

ugcucgggga caccgccugg gacuucggca gcgugggcgg cgucuucaac ucccugggga     1920

aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc auguccuggu     1980

ucagccagau ccucaucggg acgcugcugg uguggcucgg ccugaacacc aagaacggcu     2040

ccaucagccu gaccugccuc gcgcugggcg gcgugaugau cuuccugucc accgccguca     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5508
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 2 GC

<400>  5508
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugaccacga ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacg cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagaccgug gucgugcugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacgcccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcgugcug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5509
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5054/R5055 ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA 
       Design 2 GC

<400>  5509
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      120

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      180

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      240

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      360

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      420

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgugc      540

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      660

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      720

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc      780

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg      900

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc      960

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1020

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1080

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1140

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1200

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1320

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1380

ugggggacac cgcgugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5510
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 GC

<400>  5510
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      120

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg guggacgucg      180

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      240

agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      360

agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc      540

acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      660

gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc      780

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc caggaggggg      900

ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc      960

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1020

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acgcuccacg     1080

gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1140

agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg     1200

ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg caccggagcg     1320

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1380

ugggggacac ggcgugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5511
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 GC

<400>  5511
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggugau cuaccucguc augauccugc ugaucgcccc ggccuacagc auccgcugca      120

ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg gucgacgugg      180

ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc guggacaucg      240

agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga      480

agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgugc      540

acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg gccaaggugg      600

aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc agccucgggc      660

uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug acgaugaaca      720

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg uggcacgcgg      780

gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag uucaaggacg      840

cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg guccacacgg      900

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug uucuccgggc      960

accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc uacucccucu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc acggucaccg     1080

uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag auggcggugg     1140

acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc aucaccgaga     1200

gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac agcuacaucg     1260

ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1320

ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc ggggacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  5512
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 2 GC

<400>  5512
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      120

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacgucg      180

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      240

agcucgugac cacgaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      360

agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      420

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc      540

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacg gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      660

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      720

ucacgaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc      780

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcgcacgcc aagcgccaga ccguggucgu gcugggcucc caggaggggg      900

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc      960

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1020

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1080

gcacggugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugacgc ccgucgggcg ccugaucacc gcgaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggc gucggggaga agaagaucac gcaccacugg caccggagcg     1320

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1380

ugggggacac cgcgugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5513
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5180 ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA 
       Design 2 GC

<400>  5513
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuccg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5514
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 2
       GC

<400>  5514
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac acgugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccacgagc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacgcgc aagcugcaga      420

cccggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac gagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugacgacca ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacacgc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg gaacggcugc ggccucuccg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac acgggccacg     1080

agaccgacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggacgggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagacggug gucgugcugg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacgcu ccacggcacc gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacgcu gacccccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca cggagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacacggcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaacg     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcgcuggg cggcgugcug aucuuccucu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5515
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 2 GC

<400>  5515
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucgggau caucggccug cugcucacga ccgccauggc ggccgagauc acccgccggg      120

gguccgccua cuacauguac cuggaccgca gcgacgccgg caaggccauc uccuucgcga      180

ccacgcuggg cgugaacaag ugccacgugc agaucaugga ccucgggcac augugcgacg      240

ccaccaugag cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccucc acgugggugg ucuacgggac cugccaccac aagaagggcg      360

aggcccggcg cagccggcgc gccgugaccc ugcccuccca cagcacccgg aagcuccaga      420

cgcgcuccca gaccuggcug gagagccgcg aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu ccggaacccc ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg      540

gguccagcac cucccagaag gugaucuacc ucgucaugau ccugcugauc gccccggccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggga      660

cgugggucga cguggugcuc gagcacggcg gcugcgucac cgugauggcg caggacaagc      720

ccaccgugga caucgagcug gucaccacga ccguguccaa cauggccgag guccgcagcu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagcgcacg cucguggacc      900

ggggcugggg gaacggcugc gggcuguccg gcaagggcuc ccuggucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcugag cgugcacggc ucccagcaca gcgggaugau cggcuacgag acggacgagg     1080

accgggccaa gguggagguc accccgaacu ccccgcgcgc cgaggccacc cugggcgggu     1140

ucggcagccu cgggcuggac ugcgagcccc ggaccggccu ggacuucucc gaccucuacu     1200

accugacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ucccguggca cgcgggggcc gacaccggca ccccgcacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagcgcc agaccgucgu ggugcugggc agccaggagg     1380

gggcggucca cacggcccug gccggcgccc ucgaggccga gauggacggg gcgaagggcc     1440

ggcuguucuc cgggcaccug aagugccgcc ucaagaugga caagcugcgg cugaagggcg     1500

ugagcuacuc ccucugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcacggu caccguggag gugcaguacg ccgggaccga cggccccugc aagaucccgg     1620

uccagauggc gguggacaug cagacccuga cgcccguggg gcgccucauc accgccaacc     1680

ccgucaucac cgagagcacc gagaacucca agaugaugcu ggagcuggac ccgccguucg     1740

gcgacagcua caucgugauc ggggugggcg acaagaagau cacgcaccac uggcaccgga     1800

gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgcgaag cggauggccg     1860

ugcucgggga caccgccugg gacuucggca gcgugggcgg cgucuucaac ucccugggga     1920

aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc auguccuggu     1980

ucagccagau ccucaucggg acgcugcugg uguggcucgg ccugaacacc aagaacggcu     2040

ccaucagccu gaccugccuc gcgcugggcg gcgugaugau cuuccugucc accgccguca     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5516
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 2 
       GC

<400>  5516
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugaccacga ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacg cugguggacc      900

ggggcugggg gaacggcugc ggccucuccg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagaccgug gucgugcugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacgcccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcgugcug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5517
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5181 ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA 
       Design 2 GC

<400>  5517
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cguccaggac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5518
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 2
       GC

<400>  5518
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac acgugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccacgagc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacgcgc aagcugcaga      420

cccggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac gagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugacgacca ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacacgc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugcaggac acgggccacg     1080

agaccgacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggacgggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagacggug gucgugcugg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacgcu ccacggcacc gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacgcu gacccccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca cggagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacacggcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaacg     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcgcuggg cggcgugcug aucuuccucu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5519
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 2 
       GC

<400>  5519
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugaccacga ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacg cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugcaggac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagaccgug gucgugcugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacgcccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcgugcug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5520
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5182 ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 GC

<400>  5520
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc aagggcgccc     1860

agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg ggcguguuca     1920

acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc cucuucgggg     1980

gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg     2040

cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5521
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 GC

<400>  5521
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac acgugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccacgagc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacgcgc aagcugcaga      420

cccggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac gagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugacgacca ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacacgc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac acgggccacg     1080

agaccgacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggacgggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagacggug gucgugcugg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacgcu ccacggcacc gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacgcu gacccccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca cggagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc aagggcgccc     1860

agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg ggcguguuca     1920

acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc cucuucgggg     1980

gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg     2040

cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5522
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 2 GC

<400>  5522
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucgggau caucggccug cugcucacga ccgccauggc ggccgagauc acccgccggg      120

gguccgccua cuacauguac cuggaccgca gcgacgccgg caaggccauc uccuucgcga      180

ccacgcuggg cgugaacaag ugccacgugc agaucaugga ccucgggcac augugcgacg      240

ccaccaugag cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccucc acgugggugg ucuacgggac cugccaccac aagaagggcg      360

aggcccggcg cagccggcgc gccgugaccc ugcccuccca cagcacccgg aagcuccaga      420

cgcgcuccca gaccuggcug gagagccgcg aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu ccggaacccc ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg      540

gguccagcac cucccagaag gugaucuacc ucgucaugau ccugcugauc gccccggccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggga      660

cgugggucga cguggugcuc gagcacggcg gcugcgucac cgugauggcg caggacaagc      720

ccaccgugga caucgagcug gucaccacga ccguguccaa cauggccgag guccgcagcu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagcgcacg cucguggacc      900

ggggcugggg gaacggcugc gggcuguucg gcaagggcuc ccuggucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcugag cgugcacggc ucccagcaca gcgggaugau cggcuacgag acggacgagg     1080

accgggccaa gguggagguc accccgaacu ccccgcgcgc cgaggccacc cugggcgggu     1140

ucggcagccu cgggcuggac ugcgagcccc ggaccggccu ggacuucucc gaccucuacu     1200

accugacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ucccguggca cgcgggggcc gacaccggca ccccgcacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagcgcc agaccgucgu ggugcugggc agccaggagg     1380

gggcggucca cacggcccug gccggcgccc ucgaggccga gauggacggg gcgaagggcc     1440

ggcuguucuc cgggcaccug aagugccgcc ucaagaugga caagcugcgg cugaagggcg     1500

ugagcuacuc ccucugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcacggu caccguggag gugcaguacg ccgggaccga cggccccugc aagaucccgg     1620

uccagauggc gguggacaug cagacccuga cgcccguggg gcgccucauc accgccaacc     1680

ccgucaucac cgagagcacc gagaacucca agaugaugcu ggagcuggac ccgccguucg     1740

gcgacagcua caucgugauc ggggugggcg acaagaagau cacgcaccac uggcaccgga     1800

gcggcagcac ccuggggaag gccuucucca ccacgcucaa gggcgcccag cgccuggcgg     1860

cccuggggga caccgccugg gacuucggca gcaucggggg cguguucaac uccaucggcc     1920

gggccgucca ccagguguuc gggggcgccu uccgcacccu cuucgggggc augagcugga     1980

ucacccaggg gcugaugggc gcgcugcucc uguggauggg ggugaacgcc cgggaccgcu     2040

ccaucgcccu ggccuuccuc gcgacgggcg gcguccuggu guuccuggcc accaacgugc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5523
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 GC

<400>  5523
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugaccacga ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacg cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagaccgug gucgugcugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacgcccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc aagggcgccc     1860

agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg ggcguguuca     1920

acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc cucuucgggg     1980

gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg     2040

cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5524
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5618 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2
       GC

<400>  5524
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      720

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      780

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug cucuccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1680

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1740

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc gcggugagcg     2160

ccgacguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5525
<211>  2521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5525
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg caaggagaag aagcggcgcg gcgccgacac cagcgugggg aucgugggcc      120

uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc uacuacaugu      180

accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca      240

agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug uccuacgagu      300

gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc aacaccacca      360

gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc cggucccgcc      420

gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc cagaccuggc      480

ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc uuccgcaacc      540

cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc accagccaga      600

aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc cgcugcaucg      660

gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug gacaucgucc      720

uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc gacaucgagc      780

ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac gaggccucca      840

ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac cuggacaagc      900

aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg gggaacggcu      960

gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc uccaagaaga     1020

ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc uccgugcacg     1080

gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac gagaaccggg     1140

ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc ggguucggcu     1200

cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug uacuaccuca     1260

cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccgcugcccu     1320

ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc cucgucgagu     1380

ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag gagggggccg     1440

ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag ggccggcuca     1500

gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag ggggugagcu     1560

acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc cuccacggca     1620

cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc cccgcccaga     1680

uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg aaccccguca     1740

ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc uucggcgacu     1800

ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac cggagcggcu     1860

ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug gccgugcugg     1920

gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug ggcaagggga     1980

uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc ugguucagcc     2040

agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac ggcuccauca     2100

gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg     2160

acguggggug cuccgucgac uucagcaagu gaggacuagu gcaucacauu uaaaagcauc     2220

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2280

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2340

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2460

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2520

u                                                                     2521


<210>  5526
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5526
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc gugggccucc      120

ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac uacauguacc      180

ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc augaacaagu      240

gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc uacgagugcc      300

ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac accaccagca      360

cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg ucccgccggg      420

ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag accuggcucg      480

agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc cgcaacccgg      540

gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc agccagaagg      600

ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc ugcaucggcg      660

ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac aucguccugg      720

agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac aucgagcucg      780

ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag gccuccauca      840

gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug gacaagcagu      900

ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg aacggcugcg      960

gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc aagaagauga     1020

ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc gugcacggca     1080

gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag aaccgggcca     1140

agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg uucggcuccc     1200

uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac uaccucacga     1260

ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg cugcccuggc     1320

acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc gucgaguuca     1380

aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag ggggccgugc     1440

acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc cggcucagcu     1500

ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg gugagcuacu     1560

cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc cacggcacgg     1620

ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc gcccagaugg     1680

ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac cccgucauca     1740

ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu     1800

acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg agcggcucca     1860

ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc gugcuggggg     1920

acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc aaggggaucc     1980

accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg uucagccaga     2040

uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc uccaucagcc     2100

ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug agcgccgacg     2160

uggggugcuc cgucgacuuc agcaagugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  5527
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5527
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cccgcaagga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug ggccuccugc      120

ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcuccgu cgacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5528
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5528
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gcaaggagaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc cuccugcuga      120

cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug uaccucgacc      180

ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac aagugcuaca      240

uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag ugccccaugc      300

ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc agcacguggg      360

ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc cgggccguga      420

cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg cucgaguccc      480

gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac ccgggcuucg      540

cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag aaggugaucu      600

accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc ggcgucagca      660

accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc cuggagcacg      720

ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag cucgugacca      780

cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc aucagcgaca      840

uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag caguccgaca      900

cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc ugcggccucu      960

ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag augaccggga     1020

aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac ggcagccagc     1080

acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg gccaaggugg     1140

agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc ucccuggggc     1200

ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc acgaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc uggcacgccg     1320

gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc gugcacacgg     1440

cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc agcuccggcc     1500

accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc uacucccugu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc acggucaccg     1620

uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag auggccgugg     1680

acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc aucaccgaga     1740

gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac uccuacaucg     1800

ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1860

ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug ggggacaccg     1920

cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc cagauccuca     2040

ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc agccugaugu     2100

gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc gacguggggu     2160

gcuccgucga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5529
<211>  2509
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5529
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc cugcugacga      120

ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac cucgaccgga      180

acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag ugcuacaucc      240

agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc cccaugcucg      300

acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc acgugggucg      360

uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg gccgugaccc      420

ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc gagucccgcg      480

aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc      540

uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag gugaucuacc      600

uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc gucagcaacc      660

gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug gagcacgggg      720

gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc gugaccacga      780

ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc agcgacaugg      840

ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag uccgacaccc      900

aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc ggccucuucg      960

ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug accgggaagu     1020

ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc agccagcacu     1080

ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc aagguggaga     1140

ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc cuggggcucg     1200

acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg augaacaaca     1260

agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg cacgccggcg     1320

ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc     1380

acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug cacacggccc     1440

uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc uccggccacc     1500

ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac ucccugugca     1560

ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg gucaccgugg     1620

aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug gccguggaca     1680

ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc accgagagca     1740

ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc uacaucguga     1800

ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc accaucggga     1860

aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg gacaccgcgu     1920

gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc caccagaucu     1980

ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag auccucaucg     2040

gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc cugaugugcc     2100

ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac guggggugcu     2160

ccgucgacuu cagcaaguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2220

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2280

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2340

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2460

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2509


<210>  5530
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5530
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug cugacgaccg      120

ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc gaccggaacg      180

acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc uacauccaga      240

ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc augcucgacg      300

agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg ugggucgugu      360

acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc gugacccugc      420

ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag ucccgcgagu      480

acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc uucgcccugg      540

ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug aucuaccugg      600

ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc agcaaccggg      660

acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag cacgggggcu      720

gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug accacgaccg      780

ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc gacauggccu      840

ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc gacacccagu      900

acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc cucuucggga      960

agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc gggaagucca     1020

uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc cagcacuccg     1080

ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag guggagauca     1140

cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug gggcucgacu     1200

gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac gccggcgccg     1320

acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag gacgcgcacg     1380

ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac acggcccugg     1440

ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc ggccaccuga     1500

agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc cugugcaccg     1560

ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc accguggagg     1620

ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc guggacaugc     1680

agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc gagagcaccg     1740

agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac aucgugaucg     1800

gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc aucgggaagg     1860

ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac accgcguggg     1920

acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac cagaucuucg     1980

gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc cucaucggca     2040

cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug augugccucg     2100

cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacgug gggugcuccg     2160

ucgacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5531
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5531
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagcggcg cggcgccgac accagcgugg ggaucguggg ccuccugcug acgaccgcca      120

uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac cggaacgacg      180

ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac auccagauca      240

uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug cucgacgagg      300

gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg gucguguacg      360

gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug acccugccca      420

gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc cgcgaguaca      480

ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg      540

cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc uaccugguga      600

ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc aaccgggacu      660

ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac gggggcugcg      720

ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc acgaccguga      780

gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac auggccuccg      840

acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac acccaguacg      900

ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc uucgggaagg      960

gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg aaguccaucc     1020

agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag cacuccggga     1080

ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug gagaucaccc     1140

ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg cucgacugcg     1200

agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac aacaagcacu     1260

ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca     1320

ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac gcgcacgcca     1380

agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg gcccuggccg     1440

gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc caccugaagu     1500

gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug ugcaccgccg     1560

ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc guggaggugc     1620

aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug gacaugcaga     1680

cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag agcaccgaga     1740

acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc gugaucggcg     1800

ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc gggaaggccu     1860

ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc gcgugggacu     1920

ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag aucuucggcg     1980

ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc aucggcacgc     2040

ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug ugccucgccc     2100

ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg ugcuccgucg     2160

acuucagcaa gugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  5532
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5532
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agcggcgcgg cgccgacacc agcgugggga ucgugggccu ccugcugacg accgccaugg      120

cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg ggcugcguga      720

ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg accgugagca      780

acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgugu      900

gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag uccauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac uccgggauga     1080

ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg cacgccaagc     1380

gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug gaggugcagu     1620

acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac augcagaccc     1680

ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc accgagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgugg     1800

gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg     1920

gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacgcugc     2040

ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc cucgcccugg     2100

gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cguggggugc uccgucgacu     2160

ucagcaagug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  5533
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5533
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc gccauggcgg      120

ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc ugcgugaccg      720

ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc gugagcaaca      780

uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgugugca      900

agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc gggaugaucg     1080

ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc     1380

agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag gugcaguacg     1620

ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug cagacccuga     1680

cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcguggggg     1800

agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     1860

ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg gacuucggca     1920

gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acgcugcuga     2040

uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc gcccugggcg     2100

gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugcucc gucgacuuca     2160

gcaagugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5534
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5534
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggc       60

gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc auggcggccg      120

aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac gccggggagg      180

ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc auggaccugg      240

ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag ggcguggagc      300

ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac ggcacgugcc      360

accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc agccacucca      420

cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac accaagcacc      480

ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc gcggccgcca      540

ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug augauccucc      600

ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac uucguggagg      660

ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc gugaccguga      720

uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug agcaacaugg      780

ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc gacagccggu      840

gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac gugugcaagc      900

gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag ggcagccugg      960

ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc cagccggaga     1020

accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg augaucguca     1080

acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc cccaacagcc     1140

cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc gagccccgga     1200

ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac uggcuggugc     1260

acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac accgggaccc     1320

cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc aagcgccaga     1380

ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg     1440

aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag ugccgccuga     1500

agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc gccuucaccu     1560

ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug caguacgccg     1620

ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag acccugacgc     1680

ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag aacagcaaga     1740

ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc gugggggaga     1800

agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc uucgaggcca     1860

ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac uucggcagcg     1920

ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc gccgccuuca     1980

agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg cugcugaugu     2040

ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc cugggcggcg     2100

uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcuccguc gacuucagca     2160

agugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5535
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5535
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug gcggccgagg      120

ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc ggggaggcca      180

ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug gaccuggggc      240

acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc acgugccacc      360

acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc cacuccaccc      420

gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc aagcaccuga      480

uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg      540

ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug auccuccuga      600

ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc guggagggga      660

uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug accgugaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc aacauggccg      780

agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac agccggugcc      840

ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug ugcaagcgca      900

cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc agccugguga      960

ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag ccggagaacc     1020

uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug aucgucaacg     1080

acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc aacagccccc     1140

gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag ccccggaccg     1200

gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag cgccagaccg     1380

uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc gcgcuggagg     1440

ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc cgccugaaga     1500

uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag uacgccggga     1620

ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc cugacgcccg     1680

uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac agcaagauga     1740

ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug ggggagaaga     1800

agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg     1860

uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc ggcagcgugg     1920

gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug cugauguggc     2040

ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug ggcggcgucc     2100

ugaucuuccu cuccaccgcg gugagcgccg acguggggug cuccgucgac uucagcaagu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5536
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5536
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gugggccucc ugcugacgac cgccauggcg gccgagguca      120

cccggcgcgg cagcgccuac uacauguacc ucgaccggaa cgacgccggg gaggccauca      180

gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac cuggggcaca      240

ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg ugccaccaca      360

agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac uccacccgca      420

agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag caccugaucc      480

gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu      540

ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc cuccugaucg      600

cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu      660

ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc gugauggccc      720

aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac auggccgagg      780

uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc cggugcccca      840

cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc aagcgcacgc      900

uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc cuggugaccu      960

gcgccaaguu cgccugcucc aagaagauga ccgggaaguc cauccagccg gagaaccugg     1020

aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc gucaacgaca     1080

ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac agcccccgcg     1140

cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc cggaccggcc     1200

uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg accccgcacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc cagaccgugg     1380

ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg cuggaggccg     1440

agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc cugaagaugg     1500

acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc accuucacca     1560

agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac gccgggaccg     1620

acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug acgcccgugg     1680

ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc aagaugaugc     1740

ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg gagaagaaga     1800

ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc agcgugggcg     1920

gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug auguggcucg     2040

ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc ggcguccuga     2100

ucuuccucuc caccgcggug agcgccgacg uggggugcuc cgucgacuuc agcaagugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5537
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5537
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc gaggucaccc      120

ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag gccaucagcu      180

ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug gggcacaugu      240

gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag cccgacgacg      300

uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc caccacaaga      360

agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc acccgcaagc      420

ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac cugauccggg      480

ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc      540

uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc cugaucgccc      600

ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag gggauguccg      660

gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug auggcccagg      720

acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug gccgaggucc      780

gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg ugccccaccc      840

agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag cgcacgcugg      900

ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug gugaccugcg      960

ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag aaccuggagu     1020

accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc aacgacaccg     1080

gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc ccccgcgcgg     1140

aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg accggccugg     1200

acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc ccgcacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag accguggugg     1380

uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug gaggccgaga     1440

uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug aagauggaca     1500

agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc uucaccaaga     1560

uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc gggaccgacg     1620

gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg cccguggggc     1680

gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag augaugcucg     1740

agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag aagaagauca     1800

cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc gugggcgggg     1920

cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug uggcucgggc     2040

ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc guccugaucu     2100

uccucuccac cgcggugagc gccgacgugg ggugcuccgu cgacuucagc aagugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5538
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5538
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag gucacccggc      120

gcggcagcgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc aucagcuucc      180

cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg cacaugugcg      240

acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc gacgacgugg      300

acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac cacaagaagg      360

gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc cgcaagcugc      420

agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug auccgggucg      480

agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc gccuggcucc      540

uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug aucgcccccg      600

cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg auguccggcg      660

gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug gcccaggaca      720

agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc gagguccgca      780

gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc cccacccagg      840

gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc acgcuggucg      900

accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug accugcgcca      960

aguucgccug cuccaagaag augaccggga aguccaucca gccggagaac cuggaguacc     1020

gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac gacaccggcc     1080

acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc cgcgcggagg     1140

ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc ggccuggacu     1200

ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac aaggaguggu     1260

uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg cacuggaaca     1320

acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc gugguggucc     1380

ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag gccgagaugg     1440

acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag auggacaagc     1500

uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg accgacggcc     1620

cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc guggggcgcc     1680

ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug augcucgagc     1740

uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag aagaucacgc     1800

accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc guccgcggcg     1860

ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug ggcggggccc     1920

ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag agccuguucg     1980

ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg cucgggcuga     2040

acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc cugaucuucc     2100

ucuccaccgc ggugagcgcc gacguggggu gcuccgucga cuucagcaag ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5539
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5539
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccgac guggggugcu ccgucgacuu cagcaaguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5540
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5540
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgcaguc acccggcgcg      120

gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccgac guggggugcu ccgucgacuu cagcaaguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5541
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5541
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug gcggccgcag      120

ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc ggggaggcca      180

ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug gaccuggggc      240

acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc acgugccacc      360

acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc cacuccaccc      420

gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc aagcaccuga      480

uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg      540

ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug auccuccuga      600

ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc guggagggga      660

uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug accgugaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc aacauggccg      780

agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac agccggugcc      840

ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug ugcaagcgca      900

cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc agccugguga      960

ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag ccggagaacc     1020

uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug aucgucaacg     1080

acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc aacagccccc     1140

gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag ccccggaccg     1200

gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag cgccagaccg     1380

uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc gcgcuggagg     1440

ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc cgccugaaga     1500

uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag uacgccggga     1620

ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc cugacgcccg     1680

uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac agcaagauga     1740

ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug ggggagaaga     1800

agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg     1860

uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc ggcagcgugg     1920

gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug cugauguggc     2040

ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug ggcggcgucc     2100

ugaucuuccu cuccaccgcg gugagcgccg acguggggug cuccgucgac uucagcaagu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5542
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5542
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gccugagcuc ccgcaagcgg cgcagccacg acgugcucac cguccaguuc cugauccugg      120

ggaugcuccu gaugaccggc ggggucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcuccgu cgacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5543
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5543
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gccugagcuc ccgcaagcgg cgcagccacg acgugcucac cguccaguuc cugauccugg      120

ggaugcuccu gaugaccggc gcagucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcuccgu cgacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5544
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5544
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      720

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      780

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug cucuccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1680

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1740

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc gcggugagcg     2160

ccgacguggg gugcugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5545
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5545
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg caaggagaag aagcggcgcg gcgccgacac cagcgugggg aucgugggcc      120

uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc uacuacaugu      180

accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca      240

agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug uccuacgagu      300

gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc aacaccacca      360

gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc cggucccgcc      420

gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc cagaccuggc      480

ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc uuccgcaacc      540

cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc accagccaga      600

aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc cgcugcaucg      660

gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug gacaucgucc      720

uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc gacaucgagc      780

ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac gaggccucca      840

ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac cuggacaagc      900

aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg gggaacggcu      960

gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc uccaagaaga     1020

ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc uccgugcacg     1080

gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac gagaaccggg     1140

ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc ggguucggcu     1200

cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug uacuaccuca     1260

cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccgcugcccu     1320

ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc cucgucgagu     1380

ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag gagggggccg     1440

ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag ggccggcuca     1500

gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag ggggugagcu     1560

acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc cuccacggca     1620

cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc cccgcccaga     1680

uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg aaccccguca     1740

ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc uucggcgacu     1800

ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac cggagcggcu     1860

ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug gccgugcugg     1920

gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug ggcaagggga     1980

uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc ugguucagcc     2040

agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac ggcuccauca     2100

gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg     2160

acguggggug cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  5546
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5546
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc gugggccucc      120

ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac uacauguacc      180

ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc augaacaagu      240

gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc uacgagugcc      300

ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac accaccagca      360

cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg ucccgccggg      420

ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag accuggcucg      480

agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc cgcaacccgg      540

gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc agccagaagg      600

ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc ugcaucggcg      660

ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac aucguccugg      720

agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac aucgagcucg      780

ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag gccuccauca      840

gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug gacaagcagu      900

ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg aacggcugcg      960

gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc aagaagauga     1020

ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc gugcacggca     1080

gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag aaccgggcca     1140

agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg uucggcuccc     1200

uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac uaccucacga     1260

ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg cugcccuggc     1320

acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc gucgaguuca     1380

aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag ggggccgugc     1440

acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc cggcucagcu     1500

ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg gugagcuacu     1560

cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc cacggcacgg     1620

ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc gcccagaugg     1680

ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac cccgucauca     1740

ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu     1800

acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg agcggcucca     1860

ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc gugcuggggg     1920

acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc aaggggaucc     1980

accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg uucagccaga     2040

uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc uccaucagcc     2100

ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug agcgccgacg     2160

uggggugcug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  5547
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5547
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cccgcaagga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug ggccuccugc      120

ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5548
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5548
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gcaaggagaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc cuccugcuga      120

cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug uaccucgacc      180

ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac aagugcuaca      240

uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag ugccccaugc      300

ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc agcacguggg      360

ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc cgggccguga      420

cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg cucgaguccc      480

gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac ccgggcuucg      540

cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag aaggugaucu      600

accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc ggcgucagca      660

accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc cuggagcacg      720

ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag cucgugacca      780

cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc aucagcgaca      840

uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag caguccgaca      900

cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc ugcggccucu      960

ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag augaccggga     1020

aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac ggcagccagc     1080

acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg gccaaggugg     1140

agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc ucccuggggc     1200

ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc acgaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc uggcacgccg     1320

gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc gugcacacgg     1440

cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc agcuccggcc     1500

accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc uacucccugu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc acggucaccg     1620

uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag auggccgugg     1680

acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc aucaccgaga     1740

gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac uccuacaucg     1800

ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1860

ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug ggggacaccg     1920

cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc cagauccuca     2040

ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc agccugaugu     2100

gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc gacguggggu     2160

gcugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5549
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5549
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc cugcugacga      120

ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac cucgaccgga      180

acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag ugcuacaucc      240

agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc cccaugcucg      300

acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc acgugggucg      360

uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg gccgugaccc      420

ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc gagucccgcg      480

aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc      540

uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag gugaucuacc      600

uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc gucagcaacc      660

gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug gagcacgggg      720

gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc gugaccacga      780

ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc agcgacaugg      840

ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag uccgacaccc      900

aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc ggccucuucg      960

ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug accgggaagu     1020

ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc agccagcacu     1080

ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc aagguggaga     1140

ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc cuggggcucg     1200

acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg augaacaaca     1260

agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg cacgccggcg     1320

ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc     1380

acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug cacacggccc     1440

uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc uccggccacc     1500

ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac ucccugugca     1560

ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg gucaccgugg     1620

aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug gccguggaca     1680

ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc accgagagca     1740

ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc uacaucguga     1800

ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc accaucggga     1860

aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg gacaccgcgu     1920

gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc caccagaucu     1980

ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag auccucaucg     2040

gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc cugaugugcc     2100

ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac guggggugcu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5550
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5550
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug cugacgaccg      120

ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc gaccggaacg      180

acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc uacauccaga      240

ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc augcucgacg      300

agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg ugggucgugu      360

acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc gugacccugc      420

ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag ucccgcgagu      480

acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc uucgcccugg      540

ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug aucuaccugg      600

ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc agcaaccggg      660

acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag cacgggggcu      720

gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug accacgaccg      780

ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc gacauggccu      840

ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc gacacccagu      900

acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc cucuucggga      960

agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc gggaagucca     1020

uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc cagcacuccg     1080

ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag guggagauca     1140

cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug gggcucgacu     1200

gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac gccggcgccg     1320

acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag gacgcgcacg     1380

ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac acggcccugg     1440

ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc ggccaccuga     1500

agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc cugugcaccg     1560

ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc accguggagg     1620

ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc guggacaugc     1680

agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc gagagcaccg     1740

agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac aucgugaucg     1800

gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc aucgggaagg     1860

ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac accgcguggg     1920

acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac cagaucuucg     1980

gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc cucaucggca     2040

cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug augugccucg     2100

cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacgug gggugcugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5551
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5551
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagcggcg cggcgccgac accagcgugg ggaucguggg ccuccugcug acgaccgcca      120

uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac cggaacgacg      180

ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac auccagauca      240

uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug cucgacgagg      300

gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg gucguguacg      360

gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug acccugccca      420

gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc cgcgaguaca      480

ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg      540

cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc uaccugguga      600

ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc aaccgggacu      660

ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac gggggcugcg      720

ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc acgaccguga      780

gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac auggccuccg      840

acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac acccaguacg      900

ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc uucgggaagg      960

gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg aaguccaucc     1020

agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag cacuccggga     1080

ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug gagaucaccc     1140

ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg cucgacugcg     1200

agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac aacaagcacu     1260

ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca     1320

ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac gcgcacgcca     1380

agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg gcccuggccg     1440

gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc caccugaagu     1500

gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug ugcaccgccg     1560

ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc guggaggugc     1620

aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug gacaugcaga     1680

cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag agcaccgaga     1740

acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc gugaucggcg     1800

ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc gggaaggccu     1860

ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc gcgugggacu     1920

ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag aucuucggcg     1980

ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc aucggcacgc     2040

ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug ugccucgccc     2100

ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg ugcugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5552
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5552
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agcggcgcgg cgccgacacc agcgugggga ucgugggccu ccugcugacg accgccaugg      120

cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg ggcugcguga      720

ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg accgugagca      780

acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgugu      900

gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag uccauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac uccgggauga     1080

ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg cacgccaagc     1380

gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug gaggugcagu     1620

acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac augcagaccc     1680

ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc accgagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgugg     1800

gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg     1920

gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacgcugc     2040

ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc cucgcccugg     2100

gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cguggggugc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5553
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5553
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc gccauggcgg      120

ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc ugcgugaccg      720

ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc gugagcaaca      780

uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgugugca      900

agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc gggaugaucg     1080

ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc     1380

agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag gugcaguacg     1620

ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug cagacccuga     1680

cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcguggggg     1800

agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     1860

ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg gacuucggca     1920

gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acgcugcuga     2040

uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc gcccugggcg     2100

gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugcuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5554
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5554
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggc       60

gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc auggcggccg      120

aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac gccggggagg      180

ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc auggaccugg      240

ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag ggcguggagc      300

ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac ggcacgugcc      360

accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc agccacucca      420

cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac accaagcacc      480

ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc gcggccgcca      540

ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug augauccucc      600

ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac uucguggagg      660

ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc gugaccguga      720

uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug agcaacaugg      780

ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc gacagccggu      840

gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac gugugcaagc      900

gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag ggcagccugg      960

ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc cagccggaga     1020

accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg augaucguca     1080

acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc cccaacagcc     1140

cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc gagccccgga     1200

ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac uggcuggugc     1260

acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac accgggaccc     1320

cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc aagcgccaga     1380

ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg     1440

aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag ugccgccuga     1500

agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc gccuucaccu     1560

ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug caguacgccg     1620

ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag acccugacgc     1680

ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag aacagcaaga     1740

ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc gugggggaga     1800

agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc uucgaggcca     1860

ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac uucggcagcg     1920

ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc gccgccuuca     1980

agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg cugcugaugu     2040

ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc cugggcggcg     2100

uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5555
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5555
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug gcggccgagg      120

ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc ggggaggcca      180

ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug gaccuggggc      240

acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc acgugccacc      360

acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc cacuccaccc      420

gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc aagcaccuga      480

uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg      540

ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug auccuccuga      600

ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc guggagggga      660

uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug accgugaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc aacauggccg      780

agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac agccggugcc      840

ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug ugcaagcgca      900

cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc agccugguga      960

ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag ccggagaacc     1020

uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug aucgucaacg     1080

acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc aacagccccc     1140

gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag ccccggaccg     1200

gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag cgccagaccg     1380

uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc gcgcuggagg     1440

ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc cgccugaaga     1500

uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag uacgccggga     1620

ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc cugacgcccg     1680

uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac agcaagauga     1740

ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug ggggagaaga     1800

agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg     1860

uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc ggcagcgugg     1920

gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug cugauguggc     2040

ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug ggcggcgucc     2100

ugaucuuccu cuccaccgcg gugagcgccg acguggggug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5556
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5556
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gugggccucc ugcugacgac cgccauggcg gccgagguca      120

cccggcgcgg cagcgccuac uacauguacc ucgaccggaa cgacgccggg gaggccauca      180

gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac cuggggcaca      240

ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg ugccaccaca      360

agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac uccacccgca      420

agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag caccugaucc      480

gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu      540

ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc cuccugaucg      600

cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu      660

ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc gugauggccc      720

aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac auggccgagg      780

uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc cggugcccca      840

cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc aagcgcacgc      900

uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc cuggugaccu      960

gcgccaaguu cgccugcucc aagaagauga ccgggaaguc cauccagccg gagaaccugg     1020

aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc gucaacgaca     1080

ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac agcccccgcg     1140

cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc cggaccggcc     1200

uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg accccgcacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc cagaccgugg     1380

ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg cuggaggccg     1440

agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc cugaagaugg     1500

acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc accuucacca     1560

agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac gccgggaccg     1620

acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug acgcccgugg     1680

ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc aagaugaugc     1740

ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg gagaagaaga     1800

ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc agcgugggcg     1920

gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug auguggcucg     2040

ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc ggcguccuga     2100

ucuuccucuc caccgcggug agcgccgacg uggggugcug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5557
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5557
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc gaggucaccc      120

ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag gccaucagcu      180

ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug gggcacaugu      240

gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag cccgacgacg      300

uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc caccacaaga      360

agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc acccgcaagc      420

ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac cugauccggg      480

ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc      540

uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc cugaucgccc      600

ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag gggauguccg      660

gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug auggcccagg      720

acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug gccgaggucc      780

gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg ugccccaccc      840

agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag cgcacgcugg      900

ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug gugaccugcg      960

ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag aaccuggagu     1020

accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc aacgacaccg     1080

gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc ccccgcgcgg     1140

aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg accggccugg     1200

acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc ccgcacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag accguggugg     1380

uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug gaggccgaga     1440

uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug aagauggaca     1500

agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc uucaccaaga     1560

uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc gggaccgacg     1620

gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg cccguggggc     1680

gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag augaugcucg     1740

agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag aagaagauca     1800

cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc gugggcgggg     1920

cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug uggcucgggc     2040

ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc guccugaucu     2100

uccucuccac cgcggugagc gccgacgugg ggugcugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5558
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5558
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag gucacccggc      120

gcggcagcgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc aucagcuucc      180

cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg cacaugugcg      240

acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc gacgacgugg      300

acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac cacaagaagg      360

gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc cgcaagcugc      420

agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug auccgggucg      480

agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc gccuggcucc      540

uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug aucgcccccg      600

cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg auguccggcg      660

gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug gcccaggaca      720

agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc gagguccgca      780

gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc cccacccagg      840

gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc acgcuggucg      900

accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug accugcgcca      960

aguucgccug cuccaagaag augaccggga aguccaucca gccggagaac cuggaguacc     1020

gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac gacaccggcc     1080

acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc cgcgcggagg     1140

ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc ggccuggacu     1200

ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac aaggaguggu     1260

uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg cacuggaaca     1320

acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc gugguggucc     1380

ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag gccgagaugg     1440

acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag auggacaagc     1500

uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg accgacggcc     1620

cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc guggggcgcc     1680

ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug augcucgagc     1740

uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag aagaucacgc     1800

accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc guccgcggcg     1860

ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug ggcggggccc     1920

ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag agccuguucg     1980

ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg cucgggcuga     2040

acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc cugaucuucc     2100

ucuccaccgc ggugagcgcc gacguggggu gcugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5559
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5559
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccgac guggggugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5560
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5560
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgcaguc acccggcgcg      120

gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccgac guggggugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5561
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5561
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug gcggccgcag      120

ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc ggggaggcca      180

ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug gaccuggggc      240

acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc acgugccacc      360

acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc cacuccaccc      420

gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc aagcaccuga      480

uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg      540

ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug auccuccuga      600

ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc guggagggga      660

uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug accgugaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc aacauggccg      780

agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac agccggugcc      840

ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug ugcaagcgca      900

cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc agccugguga      960

ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag ccggagaacc     1020

uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug aucgucaacg     1080

acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc aacagccccc     1140

gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag ccccggaccg     1200

gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag cgccagaccg     1380

uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc gcgcuggagg     1440

ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc cgccugaaga     1500

uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag uacgccggga     1620

ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc cugacgcccg     1680

uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac agcaagauga     1740

ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug ggggagaaga     1800

agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg     1860

uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc ggcagcgugg     1920

gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug cugauguggc     2040

ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug ggcggcgucc     2100

ugaucuuccu cuccaccgcg gugagcgccg acguggggug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5562
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5562
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gccugagcuc ccgcaagcgg cgcagccacg acgugcucac cguccaguuc cugauccugg      120

ggaugcuccu gaugaccggc ggggucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5563
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5563
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gccugagcuc ccgcaagcgg cgcagccacg acgugcucac cguccaguuc cugauccugg      120

ggaugcuccu gaugaccggc gcagucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacgugg     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5564
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5564
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      720

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      780

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug cucuccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1680

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1740

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc gcggugagcg     2160

ccgacugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5565
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5565
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg caaggagaag aagcggcgcg gcgccgacac cagcgugggg aucgugggcc      120

uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc uacuacaugu      180

accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca      240

agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug uccuacgagu      300

gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc aacaccacca      360

gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc cggucccgcc      420

gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc cagaccuggc      480

ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc uuccgcaacc      540

cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc accagccaga      600

aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc cgcugcaucg      660

gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug gacaucgucc      720

uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc gacaucgagc      780

ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac gaggccucca      840

ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac cuggacaagc      900

aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg gggaacggcu      960

gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc uccaagaaga     1020

ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc uccgugcacg     1080

gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac gagaaccggg     1140

ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc ggguucggcu     1200

cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug uacuaccuca     1260

cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccgcugcccu     1320

ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc cucgucgagu     1380

ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag gagggggccg     1440

ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag ggccggcuca     1500

gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag ggggugagcu     1560

acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc cuccacggca     1620

cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc cccgcccaga     1680

uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg aaccccguca     1740

ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc uucggcgacu     1800

ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac cggagcggcu     1860

ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug gccgugcugg     1920

gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug ggcaagggga     1980

uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc ugguucagcc     2040

agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac ggcuccauca     2100

gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg     2160

acugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5566
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5566
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc gugggccucc      120

ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac uacauguacc      180

ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc augaacaagu      240

gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc uacgagugcc      300

ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac accaccagca      360

cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg ucccgccggg      420

ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag accuggcucg      480

agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc cgcaacccgg      540

gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc agccagaagg      600

ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc ugcaucggcg      660

ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac aucguccugg      720

agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac aucgagcucg      780

ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag gccuccauca      840

gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug gacaagcagu      900

ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg aacggcugcg      960

gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc aagaagauga     1020

ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc gugcacggca     1080

gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag aaccgggcca     1140

agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg uucggcuccc     1200

uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac uaccucacga     1260

ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg cugcccuggc     1320

acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc gucgaguuca     1380

aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag ggggccgugc     1440

acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc cggcucagcu     1500

ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg gugagcuacu     1560

cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc cacggcacgg     1620

ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc gcccagaugg     1680

ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac cccgucauca     1740

ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu     1800

acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg agcggcucca     1860

ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc gugcuggggg     1920

acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc aaggggaucc     1980

accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg uucagccaga     2040

uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc uccaucagcc     2100

ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug agcgccgacu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5567
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5567
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cccgcaagga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug ggccuccugc      120

ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5568
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5568
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gcaaggagaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc cuccugcuga      120

cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug uaccucgacc      180

ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac aagugcuaca      240

uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag ugccccaugc      300

ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc agcacguggg      360

ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc cgggccguga      420

cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg cucgaguccc      480

gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac ccgggcuucg      540

cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag aaggugaucu      600

accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc ggcgucagca      660

accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc cuggagcacg      720

ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag cucgugacca      780

cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc aucagcgaca      840

uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag caguccgaca      900

cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc ugcggccucu      960

ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag augaccggga     1020

aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac ggcagccagc     1080

acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg gccaaggugg     1140

agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc ucccuggggc     1200

ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc acgaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc uggcacgccg     1320

gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc gugcacacgg     1440

cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc agcuccggcc     1500

accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc uacucccugu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc acggucaccg     1620

uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag auggccgugg     1680

acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc aucaccgaga     1740

gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac uccuacaucg     1800

ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1860

ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug ggggacaccg     1920

cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc cagauccuca     2040

ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc agccugaugu     2100

gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc gacugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5569
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5569
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc cugcugacga      120

ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac cucgaccgga      180

acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag ugcuacaucc      240

agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc cccaugcucg      300

acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc acgugggucg      360

uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg gccgugaccc      420

ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc gagucccgcg      480

aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc      540

uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag gugaucuacc      600

uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc gucagcaacc      660

gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug gagcacgggg      720

gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc gugaccacga      780

ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc agcgacaugg      840

ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag uccgacaccc      900

aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc ggccucuucg      960

ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug accgggaagu     1020

ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc agccagcacu     1080

ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc aagguggaga     1140

ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc cuggggcucg     1200

acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg augaacaaca     1260

agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg cacgccggcg     1320

ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc     1380

acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug cacacggccc     1440

uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc uccggccacc     1500

ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac ucccugugca     1560

ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg gucaccgugg     1620

aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug gccguggaca     1680

ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc accgagagca     1740

ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc uacaucguga     1800

ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc accaucggga     1860

aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg gacaccgcgu     1920

gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc caccagaucu     1980

ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag auccucaucg     2040

gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc cugaugugcc     2100

ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5570
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5570
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug cugacgaccg      120

ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc gaccggaacg      180

acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc uacauccaga      240

ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc augcucgacg      300

agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg ugggucgugu      360

acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc gugacccugc      420

ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag ucccgcgagu      480

acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc uucgcccugg      540

ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug aucuaccugg      600

ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc agcaaccggg      660

acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag cacgggggcu      720

gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug accacgaccg      780

ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc gacauggccu      840

ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc gacacccagu      900

acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc cucuucggga      960

agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc gggaagucca     1020

uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc cagcacuccg     1080

ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag guggagauca     1140

cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug gggcucgacu     1200

gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac gccggcgccg     1320

acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag gacgcgcacg     1380

ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac acggcccugg     1440

ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc ggccaccuga     1500

agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc cugugcaccg     1560

ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc accguggagg     1620

ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc guggacaugc     1680

agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc gagagcaccg     1740

agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac aucgugaucg     1800

gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc aucgggaagg     1860

ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac accgcguggg     1920

acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac cagaucuucg     1980

gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc cucaucggca     2040

cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug augugccucg     2100

cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5571
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5571
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagcggcg cggcgccgac accagcgugg ggaucguggg ccuccugcug acgaccgcca      120

uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac cggaacgacg      180

ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac auccagauca      240

uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug cucgacgagg      300

gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg gucguguacg      360

gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug acccugccca      420

gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc cgcgaguaca      480

ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg      540

cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc uaccugguga      600

ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc aaccgggacu      660

ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac gggggcugcg      720

ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc acgaccguga      780

gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac auggccuccg      840

acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac acccaguacg      900

ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc uucgggaagg      960

gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg aaguccaucc     1020

agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag cacuccggga     1080

ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug gagaucaccc     1140

ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg cucgacugcg     1200

agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac aacaagcacu     1260

ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca     1320

ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac gcgcacgcca     1380

agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg gcccuggccg     1440

gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc caccugaagu     1500

gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug ugcaccgccg     1560

ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc guggaggugc     1620

aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug gacaugcaga     1680

cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag agcaccgaga     1740

acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc gugaucggcg     1800

ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc gggaaggccu     1860

ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc gcgugggacu     1920

ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag aucuucggcg     1980

ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc aucggcacgc     2040

ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug ugccucgccc     2100

ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5572
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5572
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agcggcgcgg cgccgacacc agcgugggga ucgugggccu ccugcugacg accgccaugg      120

cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg ggcugcguga      720

ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg accgugagca      780

acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgugu      900

gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag uccauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac uccgggauga     1080

ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg cacgccaagc     1380

gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug gaggugcagu     1620

acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac augcagaccc     1680

ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc accgagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgugg     1800

gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg     1920

gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacgcugc     2040

ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc cucgcccugg     2100

gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5573
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5573
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc gccauggcgg      120

ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc ugcgugaccg      720

ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc gugagcaaca      780

uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgugugca      900

agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc gggaugaucg     1080

ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc     1380

agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag gugcaguacg     1620

ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug cagacccuga     1680

cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcguggggg     1800

agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     1860

ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg gacuucggca     1920

gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acgcugcuga     2040

uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc gcccugggcg     2100

gcguccugau cuuccucucc accgcgguga gcgccgacug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5574
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5574
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggc       60

gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc auggcggccg      120

aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac gccggggagg      180

ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc auggaccugg      240

ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag ggcguggagc      300

ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac ggcacgugcc      360

accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc agccacucca      420

cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac accaagcacc      480

ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc gcggccgcca      540

ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug augauccucc      600

ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac uucguggagg      660

ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc gugaccguga      720

uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug agcaacaugg      780

ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc gacagccggu      840

gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac gugugcaagc      900

gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag ggcagccugg      960

ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc cagccggaga     1020

accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg augaucguca     1080

acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc cccaacagcc     1140

cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc gagccccgga     1200

ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac uggcuggugc     1260

acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac accgggaccc     1320

cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc aagcgccaga     1380

ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg     1440

aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag ugccgccuga     1500

agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc gccuucaccu     1560

ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug caguacgccg     1620

ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag acccugacgc     1680

ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag aacagcaaga     1740

ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc gugggggaga     1800

agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc uucgaggcca     1860

ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac uucggcagcg     1920

ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc gccgccuuca     1980

agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg cugcugaugu     2040

ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc cugggcggcg     2100

uccugaucuu ccucuccacc gcggugagcg ccgacugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5575
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5575
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug gcggccgagg      120

ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc ggggaggcca      180

ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug gaccuggggc      240

acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc acgugccacc      360

acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc cacuccaccc      420

gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc aagcaccuga      480

uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg      540

ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug auccuccuga      600

ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc guggagggga      660

uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug accgugaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc aacauggccg      780

agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac agccggugcc      840

ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug ugcaagcgca      900

cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc agccugguga      960

ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag ccggagaacc     1020

uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug aucgucaacg     1080

acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc aacagccccc     1140

gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag ccccggaccg     1200

gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag cgccagaccg     1380

uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc gcgcuggagg     1440

ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc cgccugaaga     1500

uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag uacgccggga     1620

ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc cugacgcccg     1680

uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac agcaagauga     1740

ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug ggggagaaga     1800

agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg     1860

uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc ggcagcgugg     1920

gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug cugauguggc     2040

ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug ggcggcgucc     2100

ugaucuuccu cuccaccgcg gugagcgccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5576
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5576
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gugggccucc ugcugacgac cgccauggcg gccgagguca      120

cccggcgcgg cagcgccuac uacauguacc ucgaccggaa cgacgccggg gaggccauca      180

gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac cuggggcaca      240

ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg ugccaccaca      360

agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac uccacccgca      420

agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag caccugaucc      480

gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu      540

ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc cuccugaucg      600

cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu      660

ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc gugauggccc      720

aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac auggccgagg      780

uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc cggugcccca      840

cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc aagcgcacgc      900

uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc cuggugaccu      960

gcgccaaguu cgccugcucc aagaagauga ccgggaaguc cauccagccg gagaaccugg     1020

aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc gucaacgaca     1080

ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac agcccccgcg     1140

cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc cggaccggcc     1200

uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg accccgcacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc cagaccgugg     1380

ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg cuggaggccg     1440

agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc cugaagaugg     1500

acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc accuucacca     1560

agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac gccgggaccg     1620

acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug acgcccgugg     1680

ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc aagaugaugc     1740

ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg gagaagaaga     1800

ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc agcgugggcg     1920

gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug auguggcucg     2040

ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc ggcguccuga     2100

ucuuccucuc caccgcggug agcgccgacu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5577
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5577
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc gaggucaccc      120

ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag gccaucagcu      180

ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug gggcacaugu      240

gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag cccgacgacg      300

uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc caccacaaga      360

agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc acccgcaagc      420

ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac cugauccggg      480

ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc      540

uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc cugaucgccc      600

ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag gggauguccg      660

gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug auggcccagg      720

acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug gccgaggucc      780

gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg ugccccaccc      840

agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag cgcacgcugg      900

ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug gugaccugcg      960

ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag aaccuggagu     1020

accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc aacgacaccg     1080

gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc ccccgcgcgg     1140

aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg accggccugg     1200

acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc ccgcacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag accguggugg     1380

uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug gaggccgaga     1440

uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug aagauggaca     1500

agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc uucaccaaga     1560

uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc gggaccgacg     1620

gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg cccguggggc     1680

gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag augaugcucg     1740

agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag aagaagauca     1800

cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc gugggcgggg     1920

cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug uggcucgggc     2040

ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc guccugaucu     2100

uccucuccac cgcggugagc gccgacugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  5578
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5578
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag gucacccggc      120

gcggcagcgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc aucagcuucc      180

cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg cacaugugcg      240

acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc gacgacgugg      300

acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac cacaagaagg      360

gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc cgcaagcugc      420

agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug auccgggucg      480

agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc gccuggcucc      540

uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug aucgcccccg      600

cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg auguccggcg      660

gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug gcccaggaca      720

agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc gagguccgca      780

gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc cccacccagg      840

gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc acgcuggucg      900

accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug accugcgcca      960

aguucgccug cuccaagaag augaccggga aguccaucca gccggagaac cuggaguacc     1020

gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac gacaccggcc     1080

acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc cgcgcggagg     1140

ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc ggccuggacu     1200

ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac aaggaguggu     1260

uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg cacuggaaca     1320

acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc gugguggucc     1380

ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag gccgagaugg     1440

acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag auggacaagc     1500

uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg accgacggcc     1620

cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc guggggcgcc     1680

ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug augcucgagc     1740

uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag aagaucacgc     1800

accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc guccgcggcg     1860

ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug ggcggggccc     1920

ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag agccuguucg     1980

ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg cucgggcuga     2040

acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc cugaucuucc     2100

ucuccaccgc ggugagcgcc gacugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  5579
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5579
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccgac ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5580
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5580
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgcaguc acccggcgcg      120

gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccgac ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5581
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5581
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug gcggccgcag      120

ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc ggggaggcca      180

ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug gaccuggggc      240

acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc acgugccacc      360

acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc cacuccaccc      420

gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc aagcaccuga      480

uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg      540

ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug auccuccuga      600

ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc guggagggga      660

uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug accgugaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc aacauggccg      780

agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac agccggugcc      840

ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug ugcaagcgca      900

cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc agccugguga      960

ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag ccggagaacc     1020

uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug aucgucaacg     1080

acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc aacagccccc     1140

gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag ccccggaccg     1200

gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag cgccagaccg     1380

uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc gcgcuggagg     1440

ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc cgccugaaga     1500

uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag uacgccggga     1620

ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc cugacgcccg     1680

uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac agcaagauga     1740

ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug ggggagaaga     1800

agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg     1860

uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc ggcagcgugg     1920

gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug cugauguggc     2040

ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug ggcggcgucc     2100

ugaucuuccu cuccaccgcg gugagcgccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5582
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5582
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gccugagcuc ccgcaagcgg cgcagccacg acgugcucac cguccaguuc cugauccugg      120

ggaugcuccu gaugaccggc ggggucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5583
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5583
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gccugagcuc ccgcaagcgg cgcagccacg acgugcucac cguccaguuc cugauccugg      120

ggaugcuccu gaugaccggc gcagucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5584
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5584
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      720

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      780

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug cucuccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1680

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1740

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc gcggugagcg     2160

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5585
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5585
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc gugggccucc      120

ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac uacauguacc      180

ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc augaacaagu      240

gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc uacgagugcc      300

ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac accaccagca      360

cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg ucccgccggg      420

ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag accuggcucg      480

agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc cgcaacccgg      540

gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc agccagaagg      600

ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc ugcaucggcg      660

ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac aucguccugg      720

agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac aucgagcucg      780

ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag gccuccauca      840

gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug gacaagcagu      900

ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg aacggcugcg      960

gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc aagaagauga     1020

ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc gugcacggca     1080

gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag aaccgggcca     1140

agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg uucggcuccc     1200

uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac uaccucacga     1260

ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg cugcccuggc     1320

acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc gucgaguuca     1380

aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag ggggccgugc     1440

acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc cggcucagcu     1500

ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg gugagcuacu     1560

cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc cacggcacgg     1620

ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc gcccagaugg     1680

ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac cccgucauca     1740

ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc ggcgacuccu     1800

acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg agcggcucca     1860

ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc gugcuggggg     1920

acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc aaggggaucc     1980

accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg uucagccaga     2040

uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc uccaucagcc     2100

ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug agcgccugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5586
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5586
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg caaggagaag aagcggcgcg gcgccgacac cagcgugggg aucgugggcc      120

uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc uacuacaugu      180

accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca      240

agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug uccuacgagu      300

gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc aacaccacca      360

gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc cggucccgcc      420

gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc cagaccuggc      480

ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc uuccgcaacc      540

cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc accagccaga      600

aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc cgcugcaucg      660

gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug gacaucgucc      720

uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc gacaucgagc      780

ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac gaggccucca      840

ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac cuggacaagc      900

aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg gggaacggcu      960

gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc uccaagaaga     1020

ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc uccgugcacg     1080

gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac gagaaccggg     1140

ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc ggguucggcu     1200

cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug uacuaccuca     1260

cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccgcugcccu     1320

ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc cucgucgagu     1380

ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag gagggggccg     1440

ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag ggccggcuca     1500

gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag ggggugagcu     1560

acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc cuccacggca     1620

cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc cccgcccaga     1680

uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg aaccccguca     1740

ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc uucggcgacu     1800

ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac cggagcggcu     1860

ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug gccgugcugg     1920

gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug ggcaagggga     1980

uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc ugguucagcc     2040

agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac ggcuccauca     2100

gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5587
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5587
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cccgcaagga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug ggccuccugc      120

ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5588
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5588
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gcaaggagaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc cuccugcuga      120

cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug uaccucgacc      180

ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac aagugcuaca      240

uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag ugccccaugc      300

ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc agcacguggg      360

ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc cgggccguga      420

cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg cucgaguccc      480

gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac ccgggcuucg      540

cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag aaggugaucu      600

accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc ggcgucagca      660

accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc cuggagcacg      720

ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag cucgugacca      780

cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc aucagcgaca      840

uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag caguccgaca      900

cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc ugcggccucu      960

ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag augaccggga     1020

aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac ggcagccagc     1080

acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg gccaaggugg     1140

agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc ucccuggggc     1200

ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc acgaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc uggcacgccg     1320

gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc gugcacacgg     1440

cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc agcuccggcc     1500

accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc uacucccugu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc acggucaccg     1620

uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag auggccgugg     1680

acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc aucaccgaga     1740

gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac uccuacaucg     1800

ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1860

ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug ggggacaccg     1920

cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc cagauccuca     2040

ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc agccugaugu     2100

gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5589
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5589
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

aggagaagaa gcggcgcggc gccgacacca gcguggggau cgugggccuc cugcugacga      120

ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac cucgaccgga      180

acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag ugcuacaucc      240

agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc cccaugcucg      300

acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc acgugggucg      360

uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg gccgugaccc      420

ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc gagucccgcg      480

aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc      540

uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag gugaucuacc      600

uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc gucagcaacc      660

gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug gagcacgggg      720

gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc gugaccacga      780

ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc agcgacaugg      840

ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag uccgacaccc      900

aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc ggccucuucg      960

ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug accgggaagu     1020

ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc agccagcacu     1080

ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc aagguggaga     1140

ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc cuggggcucg     1200

acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg augaacaaca     1260

agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg cacgccggcg     1320

ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc     1380

acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug cacacggccc     1440

uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc uccggccacc     1500

ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac ucccugugca     1560

ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg gucaccgugg     1620

aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug gccguggaca     1680

ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc accgagagca     1740

ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc uacaucguga     1800

ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc accaucggga     1860

aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg gacaccgcgu     1920

gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc caccagaucu     1980

ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag auccucaucg     2040

gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc cugaugugcc     2100

ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5590
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5590
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug cugacgaccg      120

ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc gaccggaacg      180

acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc uacauccaga      240

ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc augcucgacg      300

agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg ugggucgugu      360

acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc gugacccugc      420

ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag ucccgcgagu      480

acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc uucgcccugg      540

ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug aucuaccugg      600

ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc agcaaccggg      660

acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag cacgggggcu      720

gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug accacgaccg      780

ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc gacauggccu      840

ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc gacacccagu      900

acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc cucuucggga      960

agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc gggaagucca     1020

uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc cagcacuccg     1080

ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag guggagauca     1140

cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug gggcucgacu     1200

gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac gccggcgccg     1320

acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag gacgcgcacg     1380

ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac acggcccugg     1440

ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc ggccaccuga     1500

agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc cugugcaccg     1560

ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc accguggagg     1620

ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc guggacaugc     1680

agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc gagagcaccg     1740

agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac aucgugaucg     1800

gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc aucgggaagg     1860

ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac accgcguggg     1920

acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac cagaucuucg     1980

gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc cucaucggca     2040

cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug augugccucg     2100

cccugggcgg cguccugauc uuccucucca ccgcggugag cgccugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5591
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5591
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagcggcg cggcgccgac accagcgugg ggaucguggg ccuccugcug acgaccgcca      120

uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac cggaacgacg      180

ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac auccagauca      240

uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug cucgacgagg      300

gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg gucguguacg      360

gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug acccugccca      420

gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc cgcgaguaca      480

ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg      540

cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc uaccugguga      600

ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc aaccgggacu      660

ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac gggggcugcg      720

ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc acgaccguga      780

gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac auggccuccg      840

acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac acccaguacg      900

ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc uucgggaagg      960

gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg aaguccaucc     1020

agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag cacuccggga     1080

ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug gagaucaccc     1140

ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg cucgacugcg     1200

agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac aacaagcacu     1260

ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca     1320

ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac gcgcacgcca     1380

agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg gcccuggccg     1440

gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc caccugaagu     1500

gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug ugcaccgccg     1560

ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc guggaggugc     1620

aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug gacaugcaga     1680

cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag agcaccgaga     1740

acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc gugaucggcg     1800

ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc gggaaggccu     1860

ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc gcgugggacu     1920

ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag aucuucggcg     1980

ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc aucggcacgc     2040

ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug ugccucgccc     2100

ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5592
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5592
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agcggcgcgg cgccgacacc agcgugggga ucgugggccu ccugcugacg accgccaugg      120

cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg ggcugcguga      720

ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg accgugagca      780

acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgugu      900

gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag uccauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac uccgggauga     1080

ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg cacgccaagc     1380

gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug gaggugcagu     1620

acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac augcagaccc     1680

ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc accgagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgugg     1800

gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg     1920

gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacgcugc     2040

ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc cucgcccugg     2100

gcggcguccu gaucuuccuc uccaccgcgg ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5593
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5593
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

ggcgcggcgc cgacaccagc guggggaucg ugggccuccu gcugacgacc gccauggcgg      120

ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc ugcgugaccg      720

ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc gugagcaaca      780

uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgugugca      900

agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc gggaugaucg     1080

ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc     1380

agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag gugcaguacg     1620

ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug cagacccuga     1680

cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcguggggg     1800

agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     1860

ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg gacuucggca     1920

gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acgcugcuga     2040

uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc gcccugggcg     2100

gcguccugau cuuccucucc accgcgguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5594
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5594
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggc       60

gcggcgccga caccagcgug gggaucgugg gccuccugcu gacgaccgcc auggcggccg      120

aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac gccggggagg      180

ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc auggaccugg      240

ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag ggcguggagc      300

ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac ggcacgugcc      360

accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc agccacucca      420

cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac accaagcacc      480

ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc gcggccgcca      540

ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug augauccucc      600

ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac uucguggagg      660

ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc gugaccguga      720

uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug agcaacaugg      780

ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc gacagccggu      840

gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac gugugcaagc      900

gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag ggcagccugg      960

ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc cagccggaga     1020

accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg augaucguca     1080

acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc cccaacagcc     1140

cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc gagccccgga     1200

ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac uggcuggugc     1260

acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac accgggaccc     1320

cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc aagcgccaga     1380

ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg     1440

aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag ugccgccuga     1500

agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc gccuucaccu     1560

ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug caguacgccg     1620

ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag acccugacgc     1680

ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag aacagcaaga     1740

ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc gugggggaga     1800

agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc uucgaggcca     1860

ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac uucggcagcg     1920

ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc gccgccuuca     1980

agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg cugcugaugu     2040

ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc cugggcggcg     2100

uccugaucuu ccucuccacc gcggugagcg ccugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5595
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5595
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug gcggccgagg      120

ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc ggggaggcca      180

ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug gaccuggggc      240

acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc acgugccacc      360

acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc cacuccaccc      420

gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc aagcaccuga      480

uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg      540

ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug auccuccuga      600

ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc guggagggga      660

uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug accgugaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc aacauggccg      780

agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac agccggugcc      840

ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug ugcaagcgca      900

cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc agccugguga      960

ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag ccggagaacc     1020

uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug aucgucaacg     1080

acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc aacagccccc     1140

gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag ccccggaccg     1200

gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag cgccagaccg     1380

uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc gcgcuggagg     1440

ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc cgccugaaga     1500

uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag uacgccggga     1620

ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc cugacgcccg     1680

uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac agcaagauga     1740

ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug ggggagaaga     1800

agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg     1860

uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc ggcagcgugg     1920

gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug cugauguggc     2040

ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug ggcggcgucc     2100

ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5596
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5596
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gugggccucc ugcugacgac cgccauggcg gccgagguca      120

cccggcgcgg cagcgccuac uacauguacc ucgaccggaa cgacgccggg gaggccauca      180

gcuucccgac cacgcugggc augaacaagu gcuacaucca gaucauggac cuggggcaca      240

ugugcgacgc gaccaugucc uacgagugcc ccaugcucga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca cgugggucgu guacggcacg ugccaccaca      360

agaaggggga ggcccgccgg ucccgccggg ccgugacccu gcccagccac uccacccgca      420

agcugcagac gcggagccag accuggcucg agucccgcga guacaccaag caccugaucc      480

gggucgagaa cuggaucuuc cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu      540

ggcuccuggg gagcuccacc agccagaagg ugaucuaccu ggugaugauc cuccugaucg      600

cccccgcgua cuccauccgc ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu      660

ccggcggcac guggguggac aucguccugg agcacggggg cugcgugacc gugauggccc      720

aggacaagcc caccgucgac aucgagcucg ugaccacgac cgugagcaac auggccgagg      780

uccgcagcua cugcuacgag gccuccauca gcgacauggc cuccgacagc cggugcccca      840

cccaggggga ggcguaccug gacaagcagu ccgacaccca guacgugugc aagcgcacgc      900

uggucgaccg gggcuggggg aacggcugcg gccucuucgg gaagggcagc cuggugaccu      960

gcgccaaguu cgccugcucc aagaagauga ccgggaaguc cauccagccg gagaaccugg     1020

aguaccgcau caugcucucc gugcacggca gccagcacuc cgggaugauc gucaacgaca     1080

ccggccacga gacggacgag aaccgggcca agguggagau cacccccaac agcccccgcg     1140

cggaggccac ccugggcggg uucggcuccc uggggcucga cugcgagccc cggaccggcc     1200

uggacuucag cgaccuguac uaccucacga ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccg cugcccuggc acgccggcgc cgacaccggg accccgcacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgcgca cgccaagcgc cagaccgugg     1380

ugguccuggg cucccaggag ggggccgugc acacggcccu ggccggcgcg cuggaggccg     1440

agauggacgg ggccaagggc cggcucagcu ccggccaccu gaagugccgc cugaagaugg     1500

acaagcuccg gcugaagggg gugagcuacu cccugugcac cgccgccuuc accuucacca     1560

agauccccgc ggagacccuc cacggcacgg ucaccgugga ggugcaguac gccgggaccg     1620

acggcccgug caaggucccc gcccagaugg ccguggacau gcagacccug acgcccgugg     1680

ggcgccugau caccgcgaac cccgucauca ccgagagcac cgagaacagc aagaugaugc     1740

ucgagcugga cccgcccuuc ggcgacuccu acaucgugau cggcgugggg gagaagaaga     1800

ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcggauggcc gugcuggggg acaccgcgug ggacuucggc agcgugggcg     1920

gggcccucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggggg cauguccugg uucagccaga uccucaucgg cacgcugcug auguggcucg     2040

ggcugaacac caagaacggc uccaucagcc ugaugugccu cgcccugggc ggcguccuga     2100

ucuuccucuc caccgcggug agcgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  5597
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5597
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu ggggaucgug ggccuccugc ugacgaccgc cauggcggcc gaggucaccc      120

ggcgcggcag cgccuacuac auguaccucg accggaacga cgccggggag gccaucagcu      180

ucccgaccac gcugggcaug aacaagugcu acauccagau cauggaccug gggcacaugu      240

gcgacgcgac cauguccuac gagugcccca ugcucgacga gggcguggag cccgacgacg      300

uggacugcug gugcaacacc accagcacgu gggucgugua cggcacgugc caccacaaga      360

agggggaggc ccgccggucc cgccgggccg ugacccugcc cagccacucc acccgcaagc      420

ugcagacgcg gagccagacc uggcucgagu cccgcgagua caccaagcac cugauccggg      480

ucgagaacug gaucuuccgc aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc      540

uccuggggag cuccaccagc cagaagguga ucuaccuggu gaugauccuc cugaucgccc      600

ccgcguacuc cauccgcugc aucggcguca gcaaccggga cuucguggag gggauguccg      660

gcggcacgug gguggacauc guccuggagc acgggggcug cgugaccgug auggcccagg      720

acaagcccac cgucgacauc gagcucguga ccacgaccgu gagcaacaug gccgaggucc      780

gcagcuacug cuacgaggcc uccaucagcg acauggccuc cgacagccgg ugccccaccc      840

agggggaggc guaccuggac aagcaguccg acacccagua cgugugcaag cgcacgcugg      900

ucgaccgggg cugggggaac ggcugcggcc ucuucgggaa gggcagccug gugaccugcg      960

ccaaguucgc cugcuccaag aagaugaccg ggaaguccau ccagccggag aaccuggagu     1020

accgcaucau gcucuccgug cacggcagcc agcacuccgg gaugaucguc aacgacaccg     1080

gccacgagac ggacgagaac cgggccaagg uggagaucac ccccaacagc ccccgcgcgg     1140

aggccacccu gggcggguuc ggcucccugg ggcucgacug cgagccccgg accggccugg     1200

acuucagcga ccuguacuac cucacgauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccgcug cccuggcacg ccggcgccga caccgggacc ccgcacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcgcacgc caagcgccag accguggugg     1380

uccugggcuc ccaggagggg gccgugcaca cggcccuggc cggcgcgcug gaggccgaga     1440

uggacggggc caagggccgg cucagcuccg gccaccugaa gugccgccug aagauggaca     1500

agcuccggcu gaagggggug agcuacuccc ugugcaccgc cgccuucacc uucaccaaga     1560

uccccgcgga gacccuccac ggcacgguca ccguggaggu gcaguacgcc gggaccgacg     1620

gcccgugcaa gguccccgcc cagauggccg uggacaugca gacccugacg cccguggggc     1680

gccugaucac cgcgaacccc gucaucaccg agagcaccga gaacagcaag augaugcucg     1740

agcuggaccc gcccuucggc gacuccuaca ucgugaucgg cgugggggag aagaagauca     1800

cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg gauggccgug cugggggaca ccgcguggga cuucggcagc gugggcgggg     1920

cccucaacuc ccugggcaag gggauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucgggggcau guccugguuc agccagaucc ucaucggcac gcugcugaug uggcucgggc     2040

ugaacaccaa gaacggcucc aucagccuga ugugccucgc ccugggcggc guccugaucu     2100

uccucuccac cgcggugagc gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  5598
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5598
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg gaucgugggc cuccugcuga cgaccgccau ggcggccgag gucacccggc      120

gcggcagcgc cuacuacaug uaccucgacc ggaacgacgc cggggaggcc aucagcuucc      180

cgaccacgcu gggcaugaac aagugcuaca uccagaucau ggaccugggg cacaugugcg      240

acgcgaccau guccuacgag ugccccaugc ucgacgaggg cguggagccc gacgacgugg      300

acugcuggug caacaccacc agcacguggg ucguguacgg cacgugccac cacaagaagg      360

gggaggcccg ccggucccgc cgggccguga cccugcccag ccacuccacc cgcaagcugc      420

agacgcggag ccagaccugg cucgaguccc gcgaguacac caagcaccug auccgggucg      480

agaacuggau cuuccgcaac ccgggcuucg cccuggccgc ggccgccauc gccuggcucc      540

uggggagcuc caccagccag aaggugaucu accuggugau gauccuccug aucgcccccg      600

cguacuccau ccgcugcauc ggcgucagca accgggacuu cguggagggg auguccggcg      660

gcacgugggu ggacaucguc cuggagcacg ggggcugcgu gaccgugaug gcccaggaca      720

agcccaccgu cgacaucgag cucgugacca cgaccgugag caacauggcc gagguccgca      780

gcuacugcua cgaggccucc aucagcgaca uggccuccga cagccggugc cccacccagg      840

gggaggcgua ccuggacaag caguccgaca cccaguacgu gugcaagcgc acgcuggucg      900

accggggcug ggggaacggc ugcggccucu ucgggaaggg cagccuggug accugcgcca      960

aguucgccug cuccaagaag augaccggga aguccaucca gccggagaac cuggaguacc     1020

gcaucaugcu cuccgugcac ggcagccagc acuccgggau gaucgucaac gacaccggcc     1080

acgagacgga cgagaaccgg gccaaggugg agaucacccc caacagcccc cgcgcggagg     1140

ccacccuggg cggguucggc ucccuggggc ucgacugcga gccccggacc ggccuggacu     1200

ucagcgaccu guacuaccuc acgaugaaca acaagcacug gcuggugcac aaggaguggu     1260

uccacgacau cccgcugccc uggcacgccg gcgccgacac cgggaccccg cacuggaaca     1320

acaaggaggc ccucgucgag uucaaggacg cgcacgccaa gcgccagacc gugguggucc     1380

ugggcuccca ggagggggcc gugcacacgg cccuggccgg cgcgcuggag gccgagaugg     1440

acggggccaa gggccggcuc agcuccggcc accugaagug ccgccugaag auggacaagc     1500

uccggcugaa gggggugagc uacucccugu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgcggagac ccuccacggc acggucaccg uggaggugca guacgccggg accgacggcc     1620

cgugcaaggu ccccgcccag auggccgugg acaugcagac ccugacgccc guggggcgcc     1680

ugaucaccgc gaaccccguc aucaccgaga gcaccgagaa cagcaagaug augcucgagc     1740

uggacccgcc cuucggcgac uccuacaucg ugaucggcgu gggggagaag aagaucacgc     1800

accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc guccgcggcg     1860

ccaagcggau ggccgugcug ggggacaccg cgugggacuu cggcagcgug ggcggggccc     1920

ucaacucccu gggcaagggg auccaccaga ucuucggcgc cgccuucaag agccuguucg     1980

ggggcauguc cugguucagc cagauccuca ucggcacgcu gcugaugugg cucgggcuga     2040

acaccaagaa cggcuccauc agccugaugu gccucgcccu gggcggcguc cugaucuucc     2100

ucuccaccgc ggugagcgcc ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5599
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5612 ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 
       2 GC

<400>  5599
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5600
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5600
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgcaguc acccggcgcg      120

gcagcgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5601
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5601
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcgugggg aucgugggcc uccugcugac gaccgccaug gcggccgcag      120

ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc ggggaggcca      180

ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug gaccuggggc      240

acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc acgugccacc      360

acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc cacuccaccc      420

gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc aagcaccuga      480

uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg      540

ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug auccuccuga      600

ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc guggagggga      660

uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug accgugaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc aacauggccg      780

agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac agccggugcc      840

ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug ugcaagcgca      900

cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc agccugguga      960

ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag ccggagaacc     1020

uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug aucgucaacg     1080

acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc aacagccccc     1140

gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag ccccggaccg     1200

gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag cgccagaccg     1380

uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc gcgcuggagg     1440

ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc cgccugaaga     1500

uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag uacgccggga     1620

ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc cugacgcccg     1680

uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac agcaagauga     1740

ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug ggggagaaga     1800

agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg     1860

uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc ggcagcgugg     1920

gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug cugauguggc     2040

ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug ggcggcgucc     2100

ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5602
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5602
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gccugagcuc ccgcaagcgg cgcagccacg acgugcucac cguccaguuc cugauccugg      120

ggaugcuccu gaugaccggc ggggucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5603
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 GC

<400>  5603
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcggg       60

gccugagcuc ccgcaagcgg cgcagccacg acgugcucac cguccaguuc cugauccugg      120

ggaugcuccu gaugaccggc gcagucaccc ggcgcggcag cgccuacuac auguaccucg      180

accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug aacaagugcu      240

acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac gagugcccca      300

ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcacgu      360

gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc cgccgggccg      420

ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc uggcucgagu      480

cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc aacccgggcu      540

ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc cagaagguga      600

ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc aucggcguca      660

gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc guccuggagc      720

acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc gagcucguga      780

ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc uccaucagcg      840

acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac aagcaguccg      900

acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac ggcugcggcc      960

ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug cacggcagcc     1080

agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac cgggccaagg     1140

uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc ggcucccugg     1200

ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac cucacgauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug cccuggcacg     1320

ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg gccgugcaca     1440

cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg cucagcuccg     1500

gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug agcuacuccc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac ggcacgguca     1620

ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc cagauggccg     1680

uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc gucaucaccg     1740

agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc gacuccuaca     1800

ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc ggcuccacca     1860

ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug cugggggaca     1920

ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag gggauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     2040

ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc aucagccuga     2100

ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5604
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 2 GC

<400>  5604
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcucc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      720

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      780

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1680

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1740

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc gcggugagcg     2160

ccgacguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5605
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 2 GC

<400>  5605
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcucc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacacgug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

cgagcaccug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cgcgcaagcu gcagacccgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccacgagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg guggacgucg      720

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      780

agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac cuguacuacc     1260

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg     1380

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc caggaggggg     1440

ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acgcuccacg     1620

gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1680

agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg     1740

ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1920

ugggggacac ggcgugggac uucggcagcg ucggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacc cugcugaugu ggcucgggcu gaacgccaag aacggcucca     2100

ucagccugau gugccucgcg cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5606
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 2 GC

<400>  5606
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag cggaagcgcc ggggcgccga caccagcauc gggaucaucg      120

gccugcugcu cacgaccgcc auggcggccg agaucacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcaagg ccaucuccuu cgcgaccacg cugggcguga      240

acaagugcca cgugcagauc auggaccucg ggcacaugug cgacgccacc augagcuacg      300

agugcccgau gcuggacgag ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccacgug gguggucuac gggaccugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu gacccugccc ucccacagca cccggaagcu ccagacgcgc ucccagaccu      480

ggcuggagag ccgcgaguac accaagcacc ugaucaaggu ggagaacugg aucuuccgga      540

accccggcuu cgcccucguc gcgguggcca ucgccuggcu gcuggggucc agcaccuccc      600

agaaggugau cuaccucguc augauccugc ugaucgcccc ggccuacagc auccgcugca      660

ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg gucgacgugg      720

ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc uacgaggcca      840

gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga     1020

agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg gccaaggugg     1140

aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc agccucgggc     1200

uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug acgaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg uggcacgcgg     1320

gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg guccacacgg     1440

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug uucuccgggc     1500

accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc uacucccucu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc acggucaccg     1620

uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag auggcggugg     1680

acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc aucaccgaga     1740

gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac agcuacaucg     1800

ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1860

ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc ggggacaccg     1920

ccugggacuu cggcagcgug ggcggcgucu ucaacucccu ggggaagggg auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc cagauccuca     2040

ucgggacgcu gcuggugugg cucggccuga acaccaagaa cggcuccauc agccugaccu     2100

gccucgcgcu gggcggcgug augaucuucc uguccaccgc cgucagcgcc gacguggggu     2160

gcuccgugga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5607
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 2 GC

<400>  5607
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaggag aagaagcggc gcggcgccga caccagcgug gggaucgugg      120

gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcucc gccuacuaca      180

uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg cugggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc auguccuacg      300

agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      540

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      600

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      660

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacgucg      720

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      780

agcucgugac cacgaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      900

agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacg gacgagaacc     1140

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg     1200

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc     1260

ucacgaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg     1380

aguucaagga cgcgcacgcc aagcgccaga ccguggucgu gcugggcucc caggaggggg     1440

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1500

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

gcacggugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugacgc ccgucgggcg ccugaucacc gcgaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggc gucggggaga agaagaucac gcaccacugg caccggagcg     1860

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ucggcggggc ccucaacucc cugggcaagg     1980

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     2100

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gcggugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5608
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 2 GC

<400>  5608
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu gacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgga      540

accccggguu cgcccuggug gccguggcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggucau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccgcugca      660

ucgggguguc caaccgggac uucgucgagg gcaugagcgg cgggaccugg guggacgugg      720

uccuggagca cggcgggugc gugacgguga uggcccagga caagccgacc gucgacaucg      780

agcuggugac caccacggug uccaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ucacgugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucguca acgacaccgg cuacgagacc gacgagaacc     1140

gcgccaaggu ggaggugacc cccaacuccc cgcgggccga ggcgacccuc gggggcuucg     1200

ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggggccgac accggcaccc cgcacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcg aagcggcaga ccguggucgu gcucgggagc caggagggcg     1440

ccgugcacac ggcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggcaagc     1500

uguucuccgg gcaccucaag ugccgccuga agauggacaa gcuccggcug aagggcguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaaggu gcccgcggag acccuccacg     1620

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag auccccgugc     1680

agauggccgu cgacaugcag acccugacgc cgguggggcg ccugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaca agaagaucac gcaccacugg caccgguccg     1860

ggagcaccau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc cucgggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacg cugcucgugu ggcugggccu gaacaccaag aacgggucca     2100

ucagccugac cugccucgcc cugggcggcg ugaugaucuu ccucuccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5609
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 2 GC

<400>  5609
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucgugg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguca      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu cacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu ggagaacugg aucuuccgga      540

accccggguu cacccuggug gccgucgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccuggug augauccugc ucaucgcccc cgcguacagc auccgcugca      660

ucggggucuc caaccgggac uucguggagg gcaugagcgg cgggacgugg guggacgucg      720

ugcuggagca cggcgggugc gugaccguca uggcccagga caagccgacc guggacaucg      780

agcuggugac cacgaccguc uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccagc gacucccggu gccccaccca gggcgaggcg uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacgagaa ccgcgccaag guggagguca     1140

cccccaacuc cccgcgggcc gaggccaccc ucggcggguu cggcagccug gggcuggacu     1200

gcgagccccg caccggccuc gacuucuccg accuguacua ccugaccaug aacaacaagc     1260

acuggcucgu gcacaaggag ugguuccacg acaucccccu gcccuggcac gcgggcgccg     1320

acacggggac cccgcacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagcggca gaccgucgug gugcucggca gccaggaggg ggcgguccac accgcccugg     1440

ccggcgcccu ggaggcggag auggacgggg ccaagggccg ccucuucucc ggccaccuga     1500

agugccggcu gaagauggac aagcuccgcc ugaagggggu gagcuacucc cugugcacgg     1560

ccgccuucac cuucaccaag gugcccgccg agacccucca cggcaccguc acgguggagg     1620

ugcaguacgc ggggaccgac ggccccugca agguccccgc ccagauggcc guggacaugc     1680

agacccugac cccggugggc cggcugauca cggccaaccc cgucaucacc gagagcaccg     1740

agaacuccaa gaugaugcuc gagcuggacc cgcccuucgg ggacagcuac aucgugaucg     1800

gcguggggga caagaagauc acccaccacu ggcaccgcuc cggcagcacg aucgggaagg     1860

ccuucgaggc gaccguccgg ggcgccaagc gcauggccgu gcugggcgac accgccuggg     1920

acuucgggag cgugggcggg gucuucaacu cccucggcaa ggggauccac cagaucuucg     1980

gcgccgcguu caagagccug uucggcggga uguccugguu cagccagauc cugaucggca     2040

cccugcucgu guggcugggg cugaacacga agaacggcuc caucagccug accugccugg     2100

cccucggggg cgugaugauc uuccucucca ccgccgucag cgccgacgug ggcugcuccg     2160

uggacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5610
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 2 GC

<400>  5610
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accgcaagaa gggcgaggcc cggcgcagcc      420

ggcgcgcggu gacccucccc ucccacagca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggguu cgcccuggug gccguggcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggucau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccggugca      660

ucgggguguc caaccgcgac uucgucgagg gcaugagcgg cgggaccugg guggacgugg      720

uccuggagca cggcgggugc gugacgguga uggcccagga caagccgacc gucgacaucg      780

agcuggugac caccacggug uccaacaugg ccgagguccg gagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc ggacccuggu ggaccgcggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ucacgugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucguca acgacaccgg cuacgagacc gacgagaacc     1140

gggccaaggu ggaggugacc cccaacuccc cgcgcgccga ggcgacccuc gggggcuucg     1200

ggagccuggg ccuggacugc gagccccgga ccggccucga cuucuccgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggggccgac accggcaccc cgcacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcucgggagc caggagggcg     1440

ccgugcacac ggcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggcaagc     1500

uguucuccgg gcaccucaag ugccggcuga agauggacaa gcuccgccug aagggcguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaaggu gcccgcggag acccuccacg     1620

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag auccccgugc     1680

agauggccgu cgacaugcag acccugacgc cgguggggcg gcugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaca agaagaucac gcaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcga ccgugcgggg cgccaagcgc auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc cucgggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacg cugcucgugu ggcugggccu gaacaccaag aacgggucca     2100

ucagccugac cugccucgcc cugggcggcg ugaugaucuu ccucuccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5611
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 2 GC

<400>  5611
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu gacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgga      540

accccggguu cgcccuggug gccguggcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggucau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccgcugca      660

ucgggguguc caaccgggac uucgucgagg gcaugagcgg cgggaccugg guggacgugg      720

uccuggagca cggcgggugc gugacgguga uggcccagga caagccgacc gucgacaucg      780

agcuggugac caccacggug uccaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ucacgugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaccguca acgacaucgg cuacgagacc gacgagaacc     1140

gcgccaaggu ggaggugacc cccaacuccc cgcgggccga ggcgacccuc gggggcuucg     1200

ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggggccgac accggcaccc cgcacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcg aagcggcaga ccguggucgu gcucgggagc caggagggcg     1440

ccgugcacac ggcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggcaagc     1500

uguucuccgg gcaccucaag ugccgccuga agauggacaa gcuccggcug aagggcguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaaggu gcccgcggag acccuccacg     1620

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag auccccgugc     1680

agauggccgu cgacaugcag acccugacgc cgguggggcg ccugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaca agaagaucac gcaccacugg caccgguccg     1860

ggagcaccau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc cucgggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacg cugcucgugu ggcugggccu gaacaccaag aacgggucca     2100

ucagccugac cugccucgcc cugggcggcg ugaugaucuu ccucuccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5612
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 2 GC

<400>  5612
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu gacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgga      540

accccggguu cgcccuggug gccguggcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggucau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccgcugca      660

ucgggguguc caaccgggac uucgucgagg gcaugagcgg cgggaccugg guggacgugg      720

uccuggagca cggcgggugc gugacgguga uggcccagga caagccgacc gucgacaucg      780

agcuggugac caccacggug uccaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ucacgugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucggcu acgagaccga cgagaaccgc gccaaggucg     1140

aggugacccc caacuccccg cgggccgagg cgacccucgg gggcuucggg agccugggcc     1200

uggacugcga gccccgcacc ggccucgacu ucuccgaccu guacuaccug accaugaaca     1260

acaagcacug gcucgugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gggccgacac gggcaccccg cacuggaaca acaaggaggc ccuggucgag uucaaggacg     1380

cccacgcgaa gcggcagacc gugguggucc ucgggagcca ggagggcgcc gugcacaccg     1440

cccuggccgg ggcgcuggag gccgagaugg acggcgccaa gggcaagcug uucuccgggc     1500

accucaagug ccgccugaag auggacaagc uccggcugaa gggcgugagc uacucccugu     1560

gcacggccgc cuucaccuuc accaaggucc ccgcggagac ccuccacggg accgugacgg     1620

uggaggucca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1680

acaugcagac ccugaccccg gucgggcgcc ugaucacggc caaccccgug aucaccgaga     1740

gcaccgagaa cuccaagaug augcucgagc uggacccgcc cuucggcgac agcuacaucg     1800

ugaucggggu cggcgacaag aagaucaccc accacuggca ccgguccggg agcacgaucg     1860

gcaaggccuu cgaggcgacc gugcgcggcg ccaagcggau ggccgugcug ggggacaccg     1920

ccugggacuu cggcagcguc gggggcgugu ucaacucccu cgggaagggc auccaccaga     1980

ucuucggcgc cgcguucaag agccuguucg ggggcauguc cugguucagc cagauccuga     2040

ucgggacccu gcucgugugg cugggccuga acacgaagaa cggguccauc agccugaccu     2100

gccucgcccu gggcggcguc augaucuucc ucuccaccgc cgugagcgcc gacguggggu     2160

gcuccgucga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5613
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 2 GC

<400>  5613
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggcggg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5614
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 2 GC

<400>  5614
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga cgcgagcgug gggaucgucg      120

gccugcuccu gaccaccgcc auggccgccg aggugacgcg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gcgggcgagg ccaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacga      360

ccagcaccug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgcggu cacgcucccc agccacucca cccggaagcu gcagacccgc agccagaccu      480

ggcuggaguc ccgggaguac acgaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccuggcc gccgccgcga ucgccuggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacg guggacaucg      780

agcuggucac caccaccgug agcaacaugg cggaggugcg cuccuacugc uacgaggcca      840

gcaucuccga cauggccagc gacagccggu gccccacgca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugaccugcgc caaguucgcg ugcuccaaga     1020

agaugacggg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc ccgaacagcc cccgggccga ggccacgcuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggcggac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

cgguccacac cgcccuggcc ggggcccugg aggccgagau ggacggcgcg aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac cgucgaggug caguacgccg gcacggacgg ccccugcaag gugccggcgc     1680

agauggccgu cgacaugcag acccugaccc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucacgga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca cggugcgggg cgcgaagcgc auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gcggccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacaccaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacg gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5615
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 2 GC

<400>  5615
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccgggcu ggcgcuggcc gccgccgcca ucgcguggcu ccugggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccucc ugaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcucgucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccuggaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccugg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccucgacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcucgugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcuggggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcuccgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcguga     1560

gcuacucccu cugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcucaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ucggggacac cgccugggac uucggcagcg ucgggggcgc ccugaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucgggacc cugcugaugu ggcucggccu gaacacgaag aacgggucca     2100

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5616
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 2 GC

<400>  5616
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg uaccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5617
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 2 GC

<400>  5617
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgagauccg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu ggagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccguggucgu gcucgggucc caggagggcg     1440

ccgugcacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguca     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu ggacaugcag acccugaccc ccgucgggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5618
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 2 GC

<400>  5618
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugcuggccc     1680

agauggcggu cgacaugcag acccugaccc cgguggggcg gcucaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ucggggacac cgccugggac uucggcagcg ucgggggcgc ccugaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucgggacc cugcugaugu ggcucggccu gaacacgaag aacgggucca     2100

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5619
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 2 GC

<400>  5619
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgac ccuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcacgug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacgcgc agccagaccu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggacgugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacg gacgagaacc     1140

gcgcgaaggu cgagaucacc ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugacgc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac gcaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacg cuccugaugu ggcugggccu caacaccaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5620
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 2 GC

<400>  5620
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc guggugagcg     2160

ccgacguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5621
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 2 GC

<400>  5621
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcgccug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgcggu cacgcucccc agccacucca cccggaagcu gcagacccgc ccccagaccu      480

ggcuggagag ccgggaguac acgaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

acccgggguu cgcccuggcc gccgccgcga ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc cgccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacg guggacaucg      780

agcuggucac caccaccgug uccaacaugg cggaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccucc gacagccggu gccccacgca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucgcg ugcagcaaga     1020

agaugacggg caaguccauc cagccggaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacagcc cccgggccga ggccacgcuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccgcugc     1320

ccuggcacgc cggggcggac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

cgguccacac cgcccuggcc ggggcccugg aggccgagau ggacggcgcg aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac cgucgaggug caguacgccg gcacggacgg cccgugcaag gugcccgcgc     1680

agauggccgu cgacaugcag acccugaccc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucacgga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gguccaccau cggcaaggcc uucgaggcca cggugcgggg cgcgaagcgc auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gcggccuuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccugaugu ggcugggccu caacaccaag aacgggagca     2100

ucucccugau gugccuggcc cucggcggcg ugcugaucuu ccugagcacg gccguguccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5622
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 2 GC

<400>  5622
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagca      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5623
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 2 GC

<400>  5623
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccacccgcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacagc auccggugca      660

ucgggguguc caaccgcgac uucguggagg gcaugagcgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug uccaacaugg ccgaggugcg gagcuacugc uacgaggcgu      840

ccaucagcga cauggccucc gacagccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc ggacccuggu ggaccgcggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gggcgaaggu cgagaucacg ccgaacagcc cccgcgccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgga ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccggc     1500

ucuccagcgg gcaccugaag ugccgccuga agauggacaa gcuccggcug aagggcgugu     1560

ccuacagccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg ccugaucacg gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccggagcg     1860

gguccacgau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggagca     2100

ucucccugau gugccuggcc cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5624
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 2 GC

<400>  5624
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggagcgccga caccuccgug ggcaucgucg      120

ggcugcuccu gaccacggcg auggccgccg aggugacccg ccggggcagc gccuacuaca      180

uguaccugga ccgcaacgac gccggggagg cgaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccugg ggcacaugug cgacgccacg augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac ggcacgugcc accacaagaa gggggaggcc cggcgcagcc      420

ggcgcgccgu cacccucccc ucccacagca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggcuu cgcgcuggcc gccgccgcca ucgcguggcu gcucgggucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacagc auccgcugca      660

ucggcguguc caaccgggac uucguggagg ggaugagcgg cggcaccugg gucgacgugg      720

ugcuggagca cgggggcugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug uccaacaugg ccgaggugcg cagcuacugc uacgaggcgu      840

ccaucagcga cauggccagc gacucccggu gccccaccca gggggaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc uggggcaacg      960

ggugcggccu guucgggaag ggcucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg gaaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggc augaucguga acgacaccgg gcacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc ggcggguucg     1200

gcucccuggg gcuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggcgccgac acggggacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucggcagc caggaggggg     1440

ccguccacac cgcgcuggcc ggcgcccugg aggccgagau ggacggggcc aagggccgcc     1500

ucuccagcgg ccaccugaag ugccggcuga agauggacaa gcuccgccug aagggggugu     1560

ccuacagccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgugac ggucgaggug caguacgccg ggaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacacccag acccugacgc ccgugggccg gcugaucacc gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucgggg     1800

acuccuacau cgucaucggc gugggggaga agaagaucac gcaccacugg caccgcagcg     1860

gcagcaccau cgggaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggcgacac cgccugggac uucggguccg ugggcggggc ccucaacagc cugggcaagg     1980

ggauccacca gaucuucggc gccgcguuca agucccuguu cggcgggaug agcugguucu     2040

cccagauccu gaucggcacg cuccugaugu ggcuggggcu caacaccaag aacggcagca     2100

ucucccugau gugccuggcc cucgggggcg ugcugaucuu ccugagcacc gccgucuccg     2160

ccgacguggg cugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5625
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 2 GC

<400>  5625
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccaacgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggagc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucuccuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu cacccucccc ucccacagca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug uccaacaugg ccgaggugcg cagcuacugc uacgaggcgu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gguccacgau cggcaaggcc uucgaggcca ccgugcgggg cgcccgccgg auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggagca     2100

ucucccugau gugccuggcc cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5626
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 2 GC

<400>  5626
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga gaccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5627
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 2 GC

<400>  5627
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc cugcgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcucgucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccuggaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccugg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccucgacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcucgugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcuggggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcuccgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcguga     1560

gcuacucccu cugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcucaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ucggggacac cgccugggac uucggcagcg ucgggggcgc ccugaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucgggacc cugcugaugu ggcucggccu gaacacgaag aacgggucca     2100

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5628
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 2 GC

<400>  5628
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccaacgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggagc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucuccuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu cacccucccc ucccacagca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg auguuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug uccaacaugg ccgaggugcg cagcuacugc uacgaggcgu      840

ccaucagcga cauggccucc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gguccacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggagca     2100

ucucccugau gugccuggcc cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5629
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 2 GC

<400>  5629
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg auccugcgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu ccugggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccucc ugaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcucgucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccuggaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccugg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccucgacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcucgugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcuggggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcuccgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcguga     1560

gcuacucccu cugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcucaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg aucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggugc     1920

ucggggacac cgccugggac uucggcagcg ucgggggcgc ccugaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucgggacc cugcugaugu ggcucggccu gaacacgaag aacgggucca     2100

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5630
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 2 GC

<400>  5630
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucag caccaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggcga      840

cgaucuccga caucgccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgccgucgu gcucgggucc caggagggcg     1440

cggugcacac cgcccuggcc ggggcccugg aggccgagau ggacggcgcg aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugccggcgc     1680

agauggccgu ggacaugcag acccugaccc ccgucgggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgcgaagcgc auggccgugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gcggccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5631
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 2 GC

<400>  5631
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggacc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugacgc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac gcaccacugg caccgcagcg     1860

gguccaccau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacg cuccugaugu ggcugggccu caacaccaag aacgggagca     2100

ucucccugau gugccuggcc cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5632
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 2 GC

<400>  5632
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgagcug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu ggacaugcag acccugaccc ccgucgggcg gcucaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggugc     1920

ucggggacac cgccugggac uucggcagcg ucgggggcgc ccugaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucgggacc cugcugaugu ggcucggccu gaacacgaag aacgggucca     2100

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5633
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 2 GC

<400>  5633
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga gaccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcau cgagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccuggug augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggucag caaccgggac uucguggagg gcauguccgg cgggaccugg guggacgucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagcccacc guggacaucg      780

agcuggugac caccacgguc agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu ggagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccguggucgu gcucgggucc caggagggcg     1440

ccgugcacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguca     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu ggacaugcag acccugaccc ccgucgggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5634
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 2 GC

<400>  5634
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaucgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca cggugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacaccaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacg gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5635
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 2 GC

<400>  5635
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgug aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcc gcguucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgucac gguggaggug caguacgccg gcaccgacgg ccccugcaag gucccggccc     1680

agauggccgu ggacaugcag acccugaccc ccguggggcg gcugaucacg gcgaaccccg     1740

ucaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccguccgggg cgccaagcgc auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagcuucau gugccuggcg cugggcggcg ugcucaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5636
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 2 GC

<400>  5636
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggcaagc     1500

ucagcuccgg gcaccugaag ugccgccuga agauggacaa gcuccggcug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg ccugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5637
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 2 GC

<400>  5637
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga ggcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5638
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 2 GC

<400>  5638
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcgccgacgg ccccugcaag gugccggcgc     1680

agauggccgu cgacaugcag acccugaccc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucacgga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca cggugcgggg cgcgaagcgc auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gcggccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacaccaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacg gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5639
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 2 GC

<400>  5639
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc cggcaaggag aagaagcggc gcggggccga caccaacgug ggcaucgucg      120

ggcugcuccu gaccacggcg auggccgccg aggugacccg gcgcggcagc gccuacuaca      180

uguaccugga ccggaacgac gccggggagg cgaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugcccgau gcuggacgag gggguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac ggcacgugcc accacaagaa gggggaggcc cgccggagcc      420

gccgggccgu cacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucauccgggu ggagaacugg aucuuccgca      540

accccggcuu cgcgcuggcc gccgccgcca ucgcguggcu gcucgggucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacagc auccggugca      660

ucggcguguc caaccgcgac uucguggagg gcaugagcgg gggcaccugg gucgacgugg      720

ugcuggagca cgggggcugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug uccaacaugg ccgaggugcg gagcuacugc uacgaggcgu      840

ccaucagcga cauggccucc gacagccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc ggacccuggu ggaccgcggg uggggcaacg      960

ggugcggccu guucgggaag ggcucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccggaucaug cugagcgucc     1080

acggguccca gcacagcggc augaucguga acgacaccgg gcacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgcgccga ggccacccuc ggcggguucg     1200

gcucccuggg ccuggacugc gagccccgga ccgggcucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggcgccgac acggggacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcucggcagc caggaggggg     1440

ccguccacac cgcgcuggcc ggcgcccugg aggccgagau ggacggcgcc aaggggcggc     1500

ucuccagcgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aagggggugu     1560

ccuacagccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgugac ggucgaggug caguacgccg gcaccgacgg gcccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccgugggccg ccugaucacg gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucgggg     1800

acuccuacau cgucaucggc gugggggaga agaagaucac ccaccacugg caccggagcg     1860

gcuccacgau cggcaaggcc uucgaggcca ccgugcgcgg ggcccggcgc auggcggucc     1920

ugggcgacac cgccugggac uucgggagcg ugggcggggc ccucaacagc cugggcaagg     1980

gcauccacca gaucuucggg gccgcguuca agucccuguu cggcgggaug agcugguucu     2040

cccagauccu gaucggcacc cuccugaugu ggcuggggcu caacacgaag aacggcagca     2100

ucucccugau gugccuggcc cucggcgggg ugcugaucuu ccugagcacc gccgucuccg     2160

ccgacguggg cugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5640
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 2 GC

<400>  5640
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga gaccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaaggac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5641
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 2 GC

<400>  5641
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggucagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gugugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ucacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacg ccgaacagcc cccgggcgga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcccuggucg     1380

aguucaagga cgcgcacgcc aagcggcaga ccgugguggu ccucgggucc caggagggcg     1440

ccgugcacac cgcccuggcg ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcc gcguucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgucac gguggaggug caguacgccg gcaccgacgg ccccugcaag gucccggccc     1680

agauggccgu ggacaugcag acccugaccc ccguggggcg gcugaucacg gcgaaccccg     1740

ucaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccguccgggg cgccaagcgc auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcg cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5642
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 2 GC

<400>  5642
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccaacgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggagc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucuccuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacauga      360

ccuccaccug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu cacgcucccc ucccacagca cccggaagcu gcagacccgc ucccagaccu      480

ggcuggagag ccgggaguac acgaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacg guggacaucg      780

agcuggucac caccaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggcgu      840

ccaucagcga cauggccucc gacagccggu gccccacgca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacc ccgaacagcc cccgggccga ggccacgcuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcg gccuucacgu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac cgucgaggug caguacgccg gcacggacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucacgga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gguccaccau cggcaaggcc uucgaggcca cggugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccugaugu ggcugggccu caacaccaag aacgggagca     2100

ucucccugau gugccuggcc cucggcggcg ugcugaucuu ccugagcacg gccguguccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5643
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 2 GC

<400>  5643
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccgcccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggcggac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

cgguccacac cgcccuggcc ggggcccugg aggccgagau ggacggcgcg aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac cgucgaggug caguacgccg gcacggacgg ccccugcaag gugccggcgc     1680

agauggccgu cgacaugcag acccugaccc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucacgga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca cggugcgggg cgcgaagcgc auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gcggccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagacccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5644
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 2 GC

<400>  5644
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga gaccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccu ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagccgacc guggacaucg      780

agcucgucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccuggaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccugg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cucuccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg cccaacagcc cgcgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccucgacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggggccgac acgggcaccc cgcacuggaa caacaaggag gcgcucgugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcuggggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcuccgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcguga     1560

gcuacucccu cugcacggcg gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggcggu cgacaugcag acccugaccc cgguggggcg gcucaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ucggggacac cgccugggac uucggcagcg ucgggggcgc ccugaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu caucgggacc cugcugaugu ggcucggccu gaacacgaag aacgggucca     2100

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5645
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 2 GC

<400>  5645
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucgggaucag caaccgggac uucguggagg gcauguccgg cgggaccugg guggacgucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagcccacc guggacaucg      780

agcuggugac caccacgguc agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu ggagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggauccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccguggucgu gcucgggagc caggagggcg     1440

ccgugcacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgucu     1560

ccuacagccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu ggacaugcag acccugaccc ccgucgggcg gcugaucacg gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gguccacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggagca     2100

ucucccugau gugccuggcc cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacgucgg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5646
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 2 GC

<400>  5646
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacacgug cgacgccacc auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcacgug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca uccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg cuccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacgccaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacg gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5647
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 2 GC

<400>  5647
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cagcgguccc      420

gccgggccgu cacccucccc agccacucca cccgcaagcu gcagacccgg agccagacgu      480

ggcuggaguc ccgcgaguac accaagcacc ucauccgggu ggagaacugg aucuuccgca      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccggugca      660

ucggggugag caaccgcgac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug agcaacaugg ccgaggugcg guccuacugc uacgaggcga      840

gcaucuccga cauggccagc gacagccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc ggacccuggu ggaccgcggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccggaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgcgccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgga ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcucgggucc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccggc     1500

ucagcuccgg gcaccugaag ugccgccuga agauggacaa gcuccggcug aagggcguga     1560

gcuacucccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg ccugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggcggucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5648
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 2 GC

<400>  5648
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcaggcgcgc agccagaccu      480

ggcuggaguc ccgggaguac acgaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccuggcc gccgccgcga ucgccuggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacg guggacaucg      780

agcuggucac caccaccgug agcaacaugg cggaggugcg cuccuacugc uacgaggcca      840

gcaucuccga cauggccagc gacagccggu gccccacgca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugaccugcgc caaguucgcg ugcuccaaga     1020

agaugacggg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc ccgaacagcc cccgggccga ggccacgcuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggcggac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggucc caggagggcg     1440

cgguccacac cgcccuggcc ggggcccugg aggccgagau ggacggcgcg aagggccgcc     1500

ucagcuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac cgucgaggug caguacgccg gcacggacgg ccccugcaag gugccggcgc     1680

agauggccgu cgacaugcag acccugaccc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucacgga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca cggugcgggg cgcgaagcgc auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gcggccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacaccaag aacgggucca     2100

ucagccugau gugccuggcc cucggcggcg ugcugaucuu ccuguccacg gccgugagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5649
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 2 GC

<400>  5649
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcgagg cgaucuccuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu cacccucccc ucccacagca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacgguga uggcccagga caagcccacc guggacaucg      780

agcuggucac caccacggug uccaacaugg ccgaggugcg cagcuacugc uacgaggcgu      840

ccaucagcga cauggccagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgcgaaggu cgagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcgcuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcgcuggcc ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcg gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggcggu cgacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggucc     1920

ugggggacac cgccugggac uucggcuccg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgcguuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggagca     2100

ucucccugau gugccuggcc cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5650
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 2 GC

<400>  5650
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcaccga caccagcgug gggaucgucg      120

gccugcuccu gacgaccgcc auggcggccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcgagg ccaucuccuu ccccaccacg cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgcgacc augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccacgug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu cacccucccc ucccacagca cccggaagcu gcagacgcgc ucccagaccu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccuggcg gccgccgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcgcc ggccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggacgugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacg gacgagaacc     1140

gcgccaaggu cgagaucacc ccgaacagcc cccgggcgga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacac cggggccgac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcg aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acgcuccacg     1620

ggaccgugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggccgu cgacaugcag acgcugaccc ccguggggcg gcugaucacc gccaaccccg     1740

ugaucaccga gggcacggag aacuccaaga ugaugcucga gcuggacccg cccuucgggg     1800

acagcuacau cgucaucggc gugggggaga agaagaucac ccaccacugg caccgcuccg     1860

gcagcaccau cggcaaggcg uucgaggcca ccgugcgcgg ggccaagcgg auggccgucc     1920

ugggcgacac ggccugggac uucgggagcg ugggcggggu gcucaacucc cugggcaagg     1980

gcauccacca gaucuucggg gcggccuuca agagccuguu cggcgggaug uccugguuca     2040

gccagauccu gaucggcacc cuccugaugu ggcuggggcu caacaccaag aacggcucca     2100

ucagccugau gugccuggcc cucggcgggg uccugaucuu ccuguccacc gccgugagcg     2160

cggacguggg cugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5651
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 2 GC

<400>  5651
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcaacgac gccggcgagg cgaucagcuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacg auguccuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccagcaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cggcgcuccc      420

ggcgcgccgu cacccucccc agccacucca cccggaagcu gcagacccgc agccagacgu      480

ggcuggaguc ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcgcuggcc gccgccgcca ucgcguggcu gcucggcagc uccaccagcc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucacggcca uggcccagga caagcccacc guggacaucg      780

agcuggugac caccacgguc agcaacaugg cggaggugcg cuccuacugc uacgaggcca      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugacgugcgc caaguucgcg ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgugc     1080

acggcagcca gcacuccggg augcucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacg ccgaacagcc cccgggccga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cuguacuacc     1260

ucaccaugaa caacaagcac uggcuggccc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc gggggccgcc acgggcacgc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcgcacgcc aagcggcaga ccgugguggu ccuggggucc caggagggcg     1440

ccgugcacac cgcccuggcc ggggcgcucg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcuccgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcguga     1560

gcuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgcggag accgucgacg     1620

ggaccgugac gguggagggc caguacggcg ggaccgacgg ccccugcaag gucccggccc     1680

agauggccgu ggacaugcag acccugaccc ccguggggcg gcugaucacg gccaaccccg     1740

ucaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcagcacgau cgggaaggcc uucgaggcga ccguccgggg cgccaagcgc auggccgugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc gcucaacucc cugggcaagg     1980

ggauccacca gaucaucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacggcucca     2100

ucagccugau gugccuggcc cucgggggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ggggcguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5652
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 2 GC

<400>  5652
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcaccga caccagcgug gggaucgucg      120

gccugcuccu gacgaccgcc auggcggccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcgagg ccaucuccuu ccccaccacg cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgcgacc augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccacgug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu cacccucccc ucccacagca cccggaagcu gcagacgcgc ucccagaccu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccuggcg gccgccgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcgcc ggccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggacgugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacg gacgagaacc     1140

gcgccaaggu cgagaucacc ccgaacagcc cccgggcgga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcgcacgcc aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcccuggcg ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcc gcguucaccu ucuccaagau ccccgccgag acccuccacg     1620

ggaccgugac ggaggagguc caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggccgu ggacaugcag acccugaccc ccgucgggcg gcugaucacg gcgaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgcagcg     1860

gguccacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ucgggggcgc ccucaacucc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cuccugaugu ggcugggccu caacacgaag aacgggucca     2100

ucagccugau gugccuggcg cucggcggcg ugcugaucuu ccuguccacc gccgugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5653
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 2 GC

<400>  5653
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcaccga caccagcgug gggaucgucg      120

gccugcuccu gacgaccgcc auggcggccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcgagg ccaucuccuu ccccaccacg cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgcgacc augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccacgug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu cacccucccc ucccacagca cccggaagcu gcagacgcgc ucccagaccu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccuggcg gccgccgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcgcc ggccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggacgugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacg gacgagaacc     1140

gcgccaaggu cgagaucacc ccgaacagcc cccgggcgga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcgcacgcc aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcccuggcg ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcc gcguucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggccgu cgacaugcag acccugacgc ccguggggcg gcugaucacc gcgaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac gcaccacugg caccgcagcg     1860

ggagcaccau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggccgucc     1920

ugggggacac cgcgugggac uucggcuccg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacg cuccugaugu ggcugggccu caacaccaag aacgggagca     2100

ucucccugau gugccuggcg cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5654
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 2 GC

<400>  5654
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcaccga caccagcgug gggaucgucg      120

gccugcuccu gacgaccgcc auggcggugg aggugacccg ccgggggaac gccuacuaca      180

uguaccugga ccgcuccgac gccggcgagg ccaucagcuu ccccaccacg auggggauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugcccgau gcuggacgag ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcacgug gguggucuac gggaccugcc accacaagaa gggcgaggcg cggcgcuccc      420

ggcgcgccgu gacccugccc agccacucca cccggaagcu ccagacgcgc agccagaccu      480

ggcuggaguc ccgggaguac accaagcacc ugauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccucgcc gcggccgcca ucgccuggcu gcugggcagc uccaccagcc      600

agaaggucau cuaccucgug augauccugc ugaucgcccc ggcguacucc auccgcugca      660

ucggggugag caaccgggac uucgucgagg gcauguccgg cgggacgugg guggacgugg      720

uccucgagca cggcgggugc gugaccguga uggcccagga caagcccacc gucgacaucg      780

agcuggugac cacgaccgug agcaacaugg ccgagguccg cuccuacugc uacgaggcca      840

gcaucuccga cauggccagc gacagccggu gccccaccca gggcgaggcg uaccuggaca      900

agcaguccga cacccaguac gugugcaagc gcacgcucgu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccugg ugaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accucgagua ccgcaucaug cuguccgugc     1080

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc     1140

gcgccaaggu ggagaucacc ccgaacagcc cccgggccga ggcgacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccugga cuucagcgac cucuacuacc     1260

ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccucc     1320

cguggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcccuggucg     1380

aguucaagga cgcccacgcg aagcggcaga ccgugguggu ccuggggucc caggagggcg     1440

ccgugcacac cgcccucgcc ggggcgcugg aggccgagau ggacggcgcc aagggccgcc     1500

ugagcuccgg gcaccucaag ugccggcuga agauggacaa gcugcgccuc aagggcguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccugcacg     1620

ggacggucac cguggaggug caguacgccg gcaccgacgg ccccugcaag gucccggccc     1680

agauggccgu ggacaugcag acccucacgc ccguggggcg gcugaucacc gccaaccccg     1740

ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcucgacccg cccuucggcg     1800

acagcuacau cgugaucggg gugggcgaga agaagaucac gcaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcg uucgaggcca ccguccgggg cgccaagcgc auggccgugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgc gcugaacucc cucgggaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacg cugcucgucu ggcugggccu gaacaccaag aacgggucca     2100

ucagccucau gugccuggcc cugggcggcg ugcucaucuu ccuguccacc gcggugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5655
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 2 GC

<400>  5655
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcaccga caccagcgug gggaucgucg      120

gccugcuccu gacgaccgcc auggcggccg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcgagg ccaucuccuu ccccaccacg cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgcgacc augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccacgug ggugguguac gggaccugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu cacccucccc ucccacagca cccggaagcu gcagacgcgc ucccagaccu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccuggcg gccgccgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcgcc ggccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggacgugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacg gacgagaacc     1140

gcgccaaggu cgagaucacc ccgaacagcc cccgggcgga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcgcacgcc aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcccuggcg ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcc gcguucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggccgu cgacaugcag acccugacgc ccguggggcg gcugaucacc gcgaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgucaucggg gugggcgaga agaagaucac gcaccacugg caccgcagcg     1860

ggagcaccau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggccgucc     1920

ugggggacac cgcgugggac uucggcuccg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacg cuccugaugu ggcugggccu caacaccaag aacgggagca     2100

ucucccuggu gugccuggcg cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacguggg gugcagcguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5656
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 2 GC

<400>  5656
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcaccga caccagcgug gggaucgucg      120

gccugcuccu gacgaccgcc auggcggugg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcgagg ccaucuccuu ccccaccacg cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugcccgau gcuggacgag ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccacgug gguggucuac gggaccugcc accacaagaa gggcgaggcg cggcgcagcc      420

ggcgcgccgu gacccucccc ucccacagca cccggaagcu gcagacgcgc ucccagaccu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccuggcc gcggccgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggucau cuaccuggug augauccugc ucaucgcccc ggcguacagc auccgcugca      660

ucgggguguc caaccgggac uucgucgagg gcaugagcgg cgggacgugg guggacgugg      720

uccuggagca cggcgggugc gugaccguga uggcccagga caagcccgcc gucgacaucg      780

agcuggugac caccacggug uccaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ucacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacg ccgaacagcc cccgggcgga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcccuggucg     1380

aguucaagga cgcgcacgcc aagcggcaga ccgugguggu ccucgggagc caggagggcg     1440

ccgugcacac cgcccuggcg ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgugu     1560

ccuacagccu gugcaccgcc gcguucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgucac gguggaggug caguacgccg gcaccgacgg ccccugcaag gucccggccc     1680

agauggccgu ggacaugcag acccugaccc ccguggggcg gcugaucacg gcgaaccccg     1740

ucaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggg gugggcgaga agaagaucac ccaccacugg caccgcagcg     1860

ggagcacgau cggcaaggcc uucgaggcca ccguccgggg cgccaagcgc auggccgugc     1920

ugggggacac cgcgugggac uucggcuccg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccuggugu ggcugggccu caacacgaag aacgggagca     2100

ucucccugac cugccuggcg cucggcggcg ugcugaucuu ccugagcacc gccguguccg     2160

ccgacgucgg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5657
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 2 GC

<400>  5657
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgugg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcgagg ccaucuccuu ccccaccacc cucgggauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgcgacg augagcuacg      300

agugcccgau gcuggacgag ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug gguggucuac gggacgugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu gacccucccc ucccacagca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu ggagaacugg aucuuccgga      540

accccggguu cgcccuggcg gccgccguca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccuggug augauccugc ucaucgcccc ggcguacagc auccgcugca      660

ucggggucuc caaccgggac uucguggagg gcaugagcgg cgggaccugg guggacgucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagcccacc guggacaucg      780

agcuggugac caccacgguc uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccagc gacucccggu gccccaccca gggcgaggcg uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacg ccgaacagcc cccgggccga ggcgacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcg aagcggcaga ccguggucgu gcucgggagc caggagggcg     1440

ccgugcacac cgcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgucu     1560

ccuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1620

ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggccgu ggacaugcag acccugaccc ccgucgggcg gcugaucacg gccaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgcagcg     1860

ggagcacgau cggcaaggcg uucgaggcca ccgugcgggg cgccaagcgc auggccgugc     1920

ugggggacac cgccugggac uucggcuccg ucgggggcgc gcucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccuggugu ggcugggccu caacacgaag aacgggagca     2100

ucucccugac cugccuggcc cucggcggcg ugcugaucuu ccugagcacc gcggucuccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5658
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 2 GC

<400>  5658
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgugg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcgagg ccaucuccuu ccccaccacc cucgggguca      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgcgacg augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu gacccucccc ucccacagca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgga      540

accccggguu cgcccuggcg gccgccgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccuggug augauccugc ucaucgcgcc ggccuacagc auccgcugca      660

ucggggucuc caaccgggac uucguggagg gcaugagcgg cgggaccugg guggacgucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagcccacc guggacaucg      780

agcuggugac caccacgguc uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacg ccgaacagcc cccgggcgga ggccacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac acgggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcgcacgcc aagcggcaga ccguggucgu gcucgggagc caggagggcg     1440

ccgugcacac cgcccuggcg ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgucu     1560

ccuacagccu gugcaccgcc gcguucaccu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugccggccc     1680

agauggccgu ggacaugcag acccugaccc ccgucgggcg gcugaucacg gcgaaccccg     1740

ugaucaccga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggg gucggcgaca agaagaucac ccaccacugg uaccgcagcg     1860

ggagcacgau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggccgugc     1920

ugggggacac cgcgugggac uucggcuccg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccuggugu ggcugggccu caacacgaag aacgggagca     2100

ucucccugac cugccuggcg cucggcggcg ugcugaucuu ccugagcacc gccgucuccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5659
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 2 GC

<400>  5659
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag aagaagcgcc ggggcgccga caccagcgug gggaucgucg      120

gccugcuccu gaccacggcg auggccgugg aggugacccg ccgggggucc gccuacuaca      180

uguaccugga ccgcagcgac gccggcaagg ccaucuccuu ccccaccacc cucgggguca      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgcgacg augagcuacg      300

agugcccgau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgaggcc cggcgcagcc      420

ggcgcgccgu gacccucccc ucccacagca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgga      540

accccggguu cgcccuggcg gccgccgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccuggug augauccugc ucaucgcgcc ggccuacagc auccgcugca      660

ucggggucuc caaccgggac uucguggagg gcaugagcgg cgggaccugg guggacgucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagcccacc guggacaucg      780

agcuggugac caccacgguc uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggagaucacc ccgaacagcc cccgggcgga ggccacgcuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcgcacgcc aagcggcaga ccguggucgu gcucgggagc caggagggcg     1440

ccgugcacac cgcccuggcg ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccagcgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcgucu     1560

ccuacagccu gugcaccgcc gcguucacgu ucaccaagau ccccgccgag acccuccacg     1620

ggaccgugac cguggagguc caguacgccg gcacggacgg ccccugcaag gugccggccc     1680

agauggccgu ggacaugcag acccugaccc ccgucgggcg gcugaucacc gcgaaccccg     1740

ugaucacgga guccaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1800

acuccuacau cgugaucggg gucggcgaca agaagaucac ccaccacugg caccgcagcg     1860

ggagcaccau cggcaaggcc uucgaggcca cggugcgggg cgccaagcgc auggccgugc     1920

ugggggacac cgcgugggac uucggcuccg ucgggggcgc ccucaacagc cuggggaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccuguu cgggggcaug agcugguucu     2040

cccagauccu gaucgggacc cuccuggugu ggcugggccu caacaccaag aacgggagca     2100

ucucccugac gugccuggcg cucggcggcg ugcugaucuu ccugagcacc gccgucuccg     2160

ccgacguggg gugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5660
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 2 GC

<400>  5660
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucgugg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguca      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu cacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu ggagaacugg aucuuccgga      540

accccggguu cacccuggug gccgucgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccuggug augauccugc ucaucgcccc cgcguacagc auccgcugca      660

ucggggucuc caaccgggac uucguggagg gcaugagcgg cgggacgugg guggacgucg      720

ugcuggagca cggcgggugc gugaccguca uggcccagga caagccgacc guggacaucg      780

agcuggugac cacgaccguc uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggccagc gacucccggu gccccaccca gggcgaggcg uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggaggucacc cccaacuccc cgcgggccga ggccacccuc gggggcuucg     1200

ggagccuggg ccuggacugc gagccccgca cgggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcggcaga cggucguggu gcucgggagc caggagggcg     1440

cgguccacac cgcccuggcc ggggcccugg aggcggagau ggacggcgcc aagggccgcc     1500

ucuucuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acgcuccacg     1620

ggaccgucac cguggaggug caguacgcgg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu ggacaugcag acgcugaccc cgguggggcg gcugaucacc gccaaccccg     1740

ucaucaccga gagcacggag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gugggcgaca agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcga ccguccgggg cgccaagcgc auggccgugc     1920

ugggggacac ggccugggac uucggcagcg ucgggggcgu guucaacucc cucgggaagg     1980

gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cugcucgugu ggcugggccu gaacaccaag aacgggucca     2100

ucagccugac cugccucgcc cugggcggcg ugaugaucuu ccucuccacg gccgucagcg     2160

ccgacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5661
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 2 GC

<400>  5661
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccaac cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacga      360

ccuccaccug ggucguguac gggaccugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu gacgcucccc ucccacagca cccgcaagcu gcagacccgg ucccagaccu      480

ggcuggagag ccgcgaguac acgaagcacc ucaucaaggu cgagaacugg aucuuccgga      540

accccggguu cgcccuggcc gccguggcca ucgcguggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc cgccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagccgacg guggacaucg      780

agcuggucac caccaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggcgu      840

ccaucagcga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggugacc cccaacuccc cgcgggcgga ggccacgcuc gggggcuucg     1200

ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccuggucg     1380

aguucaagga cgcgcacgcc aagcggcaga ccgugguggu ccucgggagc caggagggcg     1440

ccgugcacac cgcccuggcg ggggcccugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcc gcguucacgu ucaccaaggu ccccgccgag acccuccacg     1620

ggaccgugac cguggagguc caguacgccg gcacggacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc cggucgggcg gcugaucacc gcgaaccccg     1740

ugaucacgga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca cggugcgggg cgccaagcgc auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ucgggggcgu guucaacucc cucgggaagg     1980

gcgugcacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cugcucgucu ggcugggccu gaacaccaag aacgggucca     2100

ucagccucac gugccuggcc cugggcggcg ugaugaucuu ccucuccacc gcggugagcg     2160

ccgacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5662
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 2 GC

<400>  5662
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu gacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgga      540

accccggguu cgcccuggug gccguggcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggucau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccgcugca      660

ucgggguguc caaccgggac uucgucgagg gcaugagcgg cgggaccugg guggacgugg      720

uccuggagca cggcgggugc gugacgguga uggcccagga caagccgacc gucgacaucg      780

agcuggugac caccacggug uccaacaugg ccgagguccg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ucacgugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgugc     1080

acggcuccca gcacagcggg augaucguca acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggaggugacc cccaacuccc cgcgggccga ggcgacccuc gggggcuucg     1200

ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggggccgac acgggcaccc cgcacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcg aagcggcaga ccguggucgu gcucgggagc caggagggcg     1440

ccgugcacac cgcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguca     1560

gcuacucccu gugcacggcc gccuucaccu ucaccaaggu gcccgcggag acccuccacg     1620

ggaccgugac ggucgaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu cgacaugcag acccugaccc cgguggggcg gcugaucacg gccaaccccg     1740

ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgucaucggg gugggcgaca agaagaucac ccaccacugg caccgcuccg     1860

ggagcacgau cggcaaggcg uucgaggcca ccgugcgggg cgccaagcgc auggccgucc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc cucgggaagg     1980

gcauccacca gaucuucggc gcggccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cugcucgugu ggcugggccu gaacacgaag aacgggucca     2100

ucagccugac cugccucgcc cugggcggcg ugaugaucuu ccucuccacc gccgugagcg     2160

cggacgucgg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5663
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 2 GC

<400>  5663
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccaac cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacga      360

ccuccaccug ggucguguac gggaccugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu gacgcucccc ucccacagca cccgcaagcu gcagacccgg ucccagaccu      480

ggcuggagag ccgcgaguac acgaagcacc ucaucaaggu cgagaacugg aucuuccgga      540

accccggguu cgcccuggcc gccguggcca ucgcguggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccugguc augauccugc ucaucgcccc cgccuacagc auccgcugca      660

ucgggguguc caaccgggac uucguggagg gcaugagcgg cgggaccugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagccgacg guggacaucg      780

agcuggucac caccaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggcgu      840

ccaucagcga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugaccugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacgagaa ccgcgccaag gucgagguga     1140

cccccaacuc cccgcgggcg gaggccaccc ucggcggguu cggcagccug gggcuggacu     1200

gcgagccccg cacgggccuc gacuucuccg accuguacua ccugaccaug aacaacaagc     1260

acuggcucgu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccgggac cccgcacugg aacaacaagg aggcccuggu cgaguucaag gacgcgcacg     1380

ccaagcggca gacgguggug guccucggca gccaggaggg ggccgugcac accgcccugg     1440

cgggcgcccu ggaggccgag auggacgggg ccaagggccg ccucuucucc ggccaccuga     1500

agugccggcu gaagauggac aagcuccgcc ugaagggggu gagcuacucc cugugcaccg     1560

ccgcguucac cuucacgaag guccccgccg agacccucca cggcaccgug accguggagg     1620

uccaguacgc cgggaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacgcugac cccggucggc cggcugauca ccgcgaaccc cgugaucacc gagagcacgg     1740

agaacuccaa gaugaugcuc gagcuggacc cgcccuucgg ggacagcuac aucgugaucg     1800

gcgucgggga caagaagauc acccaccacu ggcaccgcuc cggcagcacc aucgggaagg     1860

ccuucgaggc caccgugcgg ggcgccaagc gcauggccgu gcugggcgac acggcguggg     1920

acuucgggag cgucggcggg guguucaacu cccucggcaa ggggauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggga uguccugguu cagccagauc cugaucggca     2040

cccugcucgu guggcugggg cugaacacca agaacggcuc caucagccug accugccugg     2100

cccucggggg cgucaugauc uuccucucca cggcggugag cgccgacgug ggcugcuccg     2160

ucgacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5664
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 2 GC

<400>  5664
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucgugg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguca      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu cacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu ggagaacugg aucuuccgga      540

accccggguu cgcccuggug gccgucgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccgcugca      660

ucggggucuc caaccgggac uucguggagg gcaugagcgg cgggaccugg guggacgucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagccgacc guggacaucg      780

agcuggugac caccacgguc uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggaggucacc cccaacuccc cgcgggccga ggcgacccuc gggggcuucg     1200

ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggggccgac accggcaccc cgcacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcg aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac ggcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcaccgcc gccuucaccu ucaccaaggu gcccgcggag acccuccacg     1620

ggacggucac cguggaggug caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu ggacaugcag acccugacgc cgguggggcg gcugaucacc gccaaccccg     1740

ucaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcg uucgaggcca ccguccgggg cgccaagcgc auggccgugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc cucgggaagg     1980

gcauccacca gaucuucggc gcggccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacg cugcucgugu ggcugggccu gaacaccaag aacgggucca     2100

ucagccugac cugccucgcc cugggcggcg ugaugaucuu ccucuccacc gccgucagcg     2160

cggacguggg gugcuccgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5665
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 2 GC

<400>  5665
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucgugg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguca      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac gggacgugcc agcacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu cacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu ggagaacugg aucuuccgga      540

accccggguu cgcccuggug gccgucgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccgcugca      660

ucggggucuc caaccgggac uucguggagg gcaugagcgg cgggaccugg guggacgucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagccgacc guggacaucg      780

agcuggugac caccacgguc uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu ggaggucacc cccaacuccc cgcgggccga ggcgacccuc gggggcuucg     1200

ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggggccgac acgggcaccc cgcacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcg aagcggcaga ccgucguggu gcucgggagc caggagggcg     1440

ccguccacac cgcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg gcaccugaag ugccggcuga agauggacaa gcuccgccug aagggcguga     1560

gcuacucccu gugcacggcc gugugcaccg ccgcgaaggu ccccgccgag acccuccacg     1620

ggaccgugac cguggagguc caguacgccg gcacggacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc cggucgggcg gcugaucacc gcgaaccccg     1740

ugaucacgga gagcaccgag aacuccaaga ugaugcucga gcuggacccg cccuucggcg     1800

acagcuacau cgugaucggg gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcc uucgaggcca cggugcgggg cgccaagcgc auggccgugc     1920

ugggggacac cgcgugggac uucggcagcg ucgggggcgu guucaacucc cucgggaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cugcucgugu ggcugggccu gaacaccaag aacgggucca     2100

ucagccucac gugccuggcc cugggcggcg ucaugaucuu ccucuccacc gcggugagcg     2160

ccgacguggg gugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5666
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 2 GC

<400>  5666
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga cgcgagcauc gggaucgugu      120

cccugcuccu gaccaccguc auggccgccg agaucacgcg gcgcggcagc gccuacuaca      180

uguaccugga ccgguccgac gccgggaagg cgaucagcuu cgugaccacc cucggcguga      240

acaagugcca cguccagauc auggaccugg ggcacaugug cgacgccacc auguccuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacga      360

ccagcaccug ggucguguac gggaccugcc accacaagaa gggggaggcc cgccgguccc      420

gccgggccgu gacgcucccc agccacucca cccgcaagcu gcagacccgg agccagaccu      480

ggcuggaguc ccgcgaguac acgaagcacc ucaucaaggu cgagaacugg aucuuccgga      540

accccggcuu cgcgcuggug gccguggcca ucgccuggcu gcucgggagc uccaccagcc      600

agaaggucau cuaccuggug augauccugc ucaucgcccc cgcguacucc auccgcugca      660

ucggcgugag caaccgggac uucgucgagg ggauguccgg cggcaccugg guggacgugg      720

uccuggagca cgggggcugc gugaccguga uggcccagga caagccgacg gucgacaucg      780

agcuggugac caccaccgug agcaacaugg ccgagguccg cuccuacugc uacgaggcca      840

gcaucagcga cauggccucc gacagccggu gccccacgca gggggaggcg uaccucgaca      900

agcaguccga cacccaguac gugugcaagc gcacccuggu cgaccggggc uggggcaacg      960

ggugcggccu guucgggaag ggcagccucg ugaccugcgc caaguucacg ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgugc     1080

acggcagcca gcacuccggc augaucguca acgacgagaa ccgcgccaag guggagguga     1140

cccccaacag cccgcgggcc gaggccaccc ucgggggcuu cgggucccug ggccuggacu     1200

gcgagccccg cacggggcuc gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu gcccuggcac uccggcgcgg     1320

acaccgagac cccgcacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagcggca gacggugguc gugcucggca gccaggaggg ggccgugcac accgcgcugg     1440

ccggcgcccu ggaggccgag auggacgggg cgaagggccg ccucuccagc gggcaccuga     1500

agugccggcu gaagauggac aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg     1560

ccgccuucac cuucacgaag gugcccgccg agacccucca cggcaccgug accgucgagg     1620

ugcaguacgc cgggcgggac ggccccugca aggugcccgc gcagauggcc gucgacaugc     1680

agacccugac gccggugggg cgccugauca ccgccaaccc cgugaucacc gaguccaccg     1740

agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacagcuac aucgucaucg     1800

gcguggggga caagaagauc acgcaccacu ggcaccgguc cggcagcauc aucgggaagg     1860

ccuucgaggc caccgugcgc ggcgcgaagc ggauggccgu ccugggggac accgccuggg     1920

acuucggcuc cgugggcggg guguucaaca gccucggcaa ggggauccac cagaucuucg     1980

gcgccgccuu caagucccug uucgggggca ugagcugguu cucccagauc cugaucggca     2040

cccugcucgu cuggcugggg cugaacacga agaacggcag caucucccug accugccugg     2100

cgcucggggg cgugaugauc uuccucagca ccgccguguc cgccgacguc gggugcagcg     2160

uggacuucuc caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5667
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 2 GC

<400>  5667
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucgugg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguca      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggugguguac gggacgugcc accacaagaa gggcgaggcc cgccggagcc      420

gccgggcggu cacccucccc ucccacagca cccgcaagcu gcagacccgg ucccagacgu      480

ggcuggagag ccgcgaguac accaagcacc ucaucaaggu ggagaacugg aucuuccgga      540

accccggguu cgcccuggug gccgucgcca ucgccuggcu gcucggcucc agcaccuccc      600

agaaggugau cuaccuggug augauccugc ucaucgcgcc cgccuacagc auccgcugca      660

ucggggucuc caaccgggac uucguggagg gcaugagcgg cgggaccugg guggacgucg      720

ugcuggagca cggcgggugc gugacgguca uggcccagga caagccgacc guggacaucg      780

agcuggugac caccacgguc uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      840

ccaucagcga cauggcgagc gacucccggu gccccaccca gggcgaggcc uaccucgaca      900

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggucccucg ugacgugcgc caaguucacg ugcagcaaga     1020

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cugagcgucc     1080

acggcuccca gcacagcggg augaucguga acgacgagaa ccgcgccaag guggagguca     1140

cccccaacuc cccgcgggcc gaggcgaccc ucggcggguu cggcagccug gggcuggacu     1200

gcgagccccg caccggccuc gacuucuccg accuguacua ccugaccaug aacaacaagc     1260

acuggcucgu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccgggac gccgcacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

cgaagcggca gaccgucgug gugcucggca gccaggaggg ggccguccac accgcccugg     1440

ccggcgcgcu ggaggccgag auggacgggg ccaagggccg ccucuucucc ggccaccuga     1500

agugccggcu gaagauggac aagcuccgcc ugaagggggu gagcuacucc cugugcaccg     1560

ccgccuucac guucaccaag gugcccgcgg agacccucca cggcaccguc accguggagg     1620

ugcaguacgc cgggacggac ggccccugca agguccccgc ccagauggcc guggacaugc     1680

agacccugac cccggugggc cggcugauca ccgccaaccc cgucaucacg gagagcaccg     1740

agaacuccaa gaugaugcuc gagcuggacc cgcccuucgg ggacagcuac aucgugaucg     1800

gcguggggga caagaagauc acccaccacu ggcaccgcuc cggcagcacc aucgggaagg     1860

cguucgaggc cacgguccgg ggcgccaagc gcauggccgu gcugggcgac accgccuggg     1920

acuucgggag cgugggcggg gucuucaacu cccucggcaa ggggauccac cagaucuucg     1980

gcgcggccuu caagagccug uucggcggga uguccugguu cagccagauc cugaucggca     2040

cccugcucgu guggcugggg cugaacacca agaacggcuc caucagccuc acgugccugg     2100

cccucggggg cgugaugauc uuccugucca ccgccgucag cgcggacgug ggcugcuccg     2160

uggacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5668
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 2 GC

<400>  5668
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc cucgggguga      240

acaagugcca cguccagauc auggaccugg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc cggacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgagacc cgccggagcc      420

gccgguccgu gagccuccgc uaccacuaca cccggaagcu gcagacccgc ucccagacgu      480

ggcuggagag ccgggaguac aagaagcacc ucaucauggu cgagaacugg aucuuccgca      540

accccggguu cgccaucgug uccguggcga ucaccuggcu gaugggcagc cugaccuccc      600

agaaggucau cuaccucgug augaucgugc ugaucguccc cgccuacagc aucuccugca      660

ucggggugag caaccgggac cugguggagg gcauguccgg cgggaccugg gucgacgugg      720

ugcucgagca cggcgggugc gucacggaga uggcccagga caagcccacc guggacaucg      780

agcuggugac caugaccguc agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      840

gccugagcga cauggcgucc gccagccggu gcccgacgca gggcgagccc ucccucgaca      900

agcagagcga cacccagucc gugugcaagc gcacccuggg cgaccggggg uggggcaacg      960

ggugcggcau cuucgggaag ggcagccugg ugaccugcuc caaguucacg ugcugcaaga     1020

agaugcccgg caagagcauc cagcccgaga accucgagua ccgcaucaug cugccggucc     1080

acggguccca gcacagcggc augaucguca acgacaucgg gcacgagacc gacgagaacc     1140

gcgccaaggu ggaggugacc cccaacagcc cccgggccga ggccacccug ggcggguucg     1200

gcucccucgg ccuggacugc gagccccgca cggggcugga cuucuccgac cucuacuacc     1260

ugaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcucc     1320

ccuggcacgc gggcgccgac accgggacgc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagcggcaga ccguggucgu gcugggcagc caggaggggg     1440

cggugcacac cgcccucgcc ggcgcccugg aggccgagau ggacggcgcg aaggggcgcc     1500

uguucuccgg ccaccucaag ugccggcuga agauggacaa gcugcgccuc aaggggguca     1560

gcuacucccu gugcaccgcc gccuucaccu ucacgaaggu gcccgccgag acccugcacg     1620

gcaccgugac cgucgaggug cagagcgcgg gcaccgacgg gccgugcaag gugcccgccc     1680

agauggccgu cgacaugcag acgcucaccc ccgugggccg gcugaucacc gccaaccccg     1740

ugaucaccga guccacggag aacagcaaga ugaugcugga gcucgacccg cccuucgggg     1800

acagcuacau cgucaucggc gugggggaca agaagaucac ccaccacugg caccgcuccg     1860

gcagcaccau cggcaaggcc uucgaggcga ccgugcgcgg ggccaagcgg auggccgucc     1920

ugggcgacac ggccugggac uucggguccg ugggcggggu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggg gccgcguuca agucccucuu cggcgggaug agcugguucu     2040

cccagauccu gaucggcacc cugcuggucu ggcucgggcu gaacaccaag aacggcagca     2100

ucucccugac cugccucgcc cugggcgggg ugaugaucuu ccugagcacg gccguguccg     2160

ccgacgucgg cugcagcgug gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5669
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 2 GC

<400>  5669
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccggaaggag cgcaagcggc gcggcgccga caccagcauc gggaucaucg      120

gccugcuccu gaccacggcg auggccgccg agaucacccg gcgcgggucc gccuacuaca      180

uguaccugga ccggagcgac gccggcaagg cgaucuccuu cgccaccacc auggggguga      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacg augagcuacg      300

agugccccau gcuggacgag ggcguggagc cggacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggacgugcc accacaagaa gggcgaggcc cgccccagcc      420

ggcgcgcggu gacccugccc ucccacagca cccggaagcu ccagacccgc ucccagacgu      480

ggcuggagag ccgggaguac accaagcacc ugaucaaggu cgagaacugg auccuccgca      540

accccggguu caccguggcc gcccuggcca uggccuggcu gcucggcaac uccaccagcc      600

agcgggugau cuaccugguc augauccugc ucaucgcgcc ggccuacucc auccgcugca      660

ucggggugag caaccgggac uucguggagg gcauguccgg cgggacgugg gucgacgugg      720

ugcuggagca cggcgggugc gucaccguga uggcccagga caagcccacc guggacaucg      780

agcuggucac cacgaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      840

gcaucuccga cauggcgagc gacagccggu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      960

gcugcgggcu guucggcaag gggagccucg ugaccugcgc caaguucacg ugcuccaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua ccgcaucaug cuguccgucc     1080

acggcagcca gcacuccggg augaucguga acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggugacc ccgaacagcc cccgggccga ggcgacccuc gggggcuucg     1200

ggucccuggg ccuggacugc gagccccgca ccggccucga cuucagcgac cuguacuacc     1260

ugacgaugaa caacaagcac uggcucgugc acaaggagug guuccacgac aucccccugc     1320

cguggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcccuggucg     1380

aguucaagga cgcccacgcg aagcggcaga ccgugguggu ccucgggucc caggagggcg     1440

ccgugcacac cgcccuggcc ggggcgcugg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucagcgg gcaccugaag ugccggauca agauggacaa gcugcgccuc aagggcgugu     1560

ccuacagccu gugcaccgcc gccuucaccu ucuccaaggu ccccgcggag acccugcacg     1620

ggaccgugac gguggagguc caguacgccg gccgggacgg ccccugcaag gugccggccc     1680

agauggccgu ggacaugcag acccucaccc ccgucgggcg ccugaucacc gccaacccca     1740

ucaucacgga gagcaccgag aacucccgga ugaugcugga gcucgacccg cccuucggcg     1800

acagcuacau cgugaucggg gugggcgaca agaagaucac ccaccacugg caccgcuccg     1860

ggagcaccau cggcaaggcg uucgaggcca cgguccgggg cgccaagcgc auggccgugc     1920

ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc cuggggaagg     1980

gcgugcacca gguguucggc gcggccuuca agagccucuu cgggggcaug uccugguuca     2040

gccagauccu gaucgggacc cugcuggucu ggcucggccu gaacaccaag aacgggucca     2100

ucagccuggc cugccucgcc cugggcggcg cgaugaucuu ccuguccacg gccgugagcg     2160

ccgacguggg gugcuccguc gacuucugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  5670
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 2 GC

<400>  5670
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5671
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 2 GC

<400>  5671
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac acgugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccacgagc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacgcgc aagcugcaga      420

cccggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac gagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugacgacca ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacacgc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac acgggccacg     1080

agaccgacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggacgggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagacggug gucgugcugg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacgcu ccacggcacc gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacgcu gacccccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca cggagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacacggcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaacg     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcgcuggg cggcgugcug aucuuccucu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5672
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 2 GC

<400>  5672
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucgggau caucggccug cugcucacga ccgccauggc ggccgagauc acccgccggg      120

gguccgccua cuacauguac cuggaccgca gcgacgccgg caaggccauc uccuucgcga      180

ccacgcuggg cgugaacaag ugccacgugc agaucaugga ccucgggcac augugcgacg      240

ccaccaugag cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccucc acgugggugg ucuacgggac cugccaccac aagaagggcg      360

aggcccggcg cagccggcgc gccgugaccc ugcccuccca cagcacccgg aagcuccaga      420

cgcgcuccca gaccuggcug gagagccgcg aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu ccggaacccc ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg      540

gguccagcac cucccagaag gugaucuacc ucgucaugau ccugcugauc gccccggccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggga      660

cgugggucga cguggugcuc gagcacggcg gcugcgucac cgugauggcg caggacaagc      720

ccaccgugga caucgagcug gucaccacga ccguguccaa cauggccgag guccgcagcu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagcgcacg cucguggacc      900

ggggcugggg gaacggcugc gggcuguucg gcaagggcuc ccuggucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcugag cgugcacggc ucccagcaca gcgggaugau cggcuacgag acggacgagg     1080

accgggccaa gguggagguc accccgaacu ccccgcgcgc cgaggccacc cugggcgggu     1140

ucggcagccu cgggcuggac ugcgagcccc ggaccggccu ggacuucucc gaccucuacu     1200

accugacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ucccguggca cgcgggggcc gacaccggca ccccgcacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagcgcc agaccgucgu ggugcugggc agccaggagg     1380

gggcggucca cacggcccug gccggcgccc ucgaggccga gauggacggg gcgaagggcc     1440

ggcuguucuc cgggcaccug aagugccgcc ucaagaugga caagcugcgg cugaagggcg     1500

ugagcuacuc ccucugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcacggu caccguggag gugcaguacg ccgggaccga cggccccugc aagaucccgg     1620

uccagauggc gguggacaug cagacccuga cgcccguggg gcgccucauc accgccaacc     1680

ccgucaucac cgagagcacc gagaacucca agaugaugcu ggagcuggac ccgccguucg     1740

gcgacagcua caucgugauc ggggugggcg acaagaagau cacgcaccac uggcaccgga     1800

gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgcgaag cggauggccg     1860

ugcucgggga caccgccugg gacuucggca gcgugggcgg cgucuucaac ucccugggga     1920

aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc auguccuggu     1980

ucagccagau ccucaucggg acgcugcugg uguggcucgg ccugaacacc aagaacggcu     2040

ccaucagccu gaccugccuc gcgcugggcg gcgugaugau cuuccugucc accgccguca     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5673
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 2 GC

<400>  5673
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgagguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugaccacga ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacg cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagaccgug gucgugcugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacgcccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcgugcug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5674
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 2 GC

<400>  5674
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucgggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgugaacaag ugccacgucc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgugaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ugguggcggu ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gucaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc guguccaacc gcgacuucgu cgaggggaug agcggcggca      660

ccugggugga cgugguccug gagcacgggg gcugcgugac ggugauggcg caggacaagc      720

cgaccgucga caucgagcug gugaccacca cgguguccaa cauggccgag guccggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacc cugguggacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cgugcacggc ucccagcaca gcggcaugau cgucaacgac accggguacg     1080

agaccgacga gaaccgcgcc aagguggagg ugacccccaa cuccccgcgg gccgaggcca     1140

cccucggcgg guucggcagc cuggggcugg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccugua cuaccugacg augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg cggacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg gcagaccgug gucgugcucg     1380

gcagccagga gggggccgug cacacggcgc uggccggcgc ccuggaggcc gagauggacg     1440

gggcgaaggg caagcuguuc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

gccugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu ccacggcacg gugaccgucg aggugcagua cgccgggacc gacggccccu     1620

gcaagauccc cgugcagaug gcggucgaca ugcagacccu gacgccggug ggccggcuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggacaagaag aucacgcacc     1800

acuggcaccg cuccggcagc accaucggga aggccuucga ggccaccgug cggggcgcca     1860

agcgcauggc gguccugggc gacaccgccu gggacuucgg gagcgugggc gggguguuca     1920

acucccucgg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacgcugcu cgucuggcug gggcugaaca     2040

ccaagaacgg cuccaucagc cugaccugcc ucgcccucgg gggcgugaug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5675
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 2 GC

<400>  5675
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucgggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgugaacaag ugccacgucc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggucg uguacggcac gugccaccgg aagaaggggg      360

aggcccgccg gagccgccgg gccgugaccc uccccuccca cagcacccgc aagcugcaga      420

cccgguccca gacguggcug gagagccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccggaacccc ggcuucgccc ugguggcggu ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gucaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu cgaggggaug agcggcggca      660

ccugggugga cgugguccug gagcacgggg gcugcgugac ggugauggcg caggacaagc      720

cgaccgucga caucgagcug gugaccacca cgguguccaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cgugcacggc ucccagcaca gcggcaugau cgucaacgac accggguacg     1080

agaccgacga gaaccgggcc aagguggagg ugacccccaa cuccccgcgc gccgaggcca     1140

cccucggcgg guucggcagc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacg augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg cggacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccgug gucgugcucg     1380

gcagccagga gggggccgug cacacggcgc uggccggcgc ccuggaggcc gagauggacg     1440

gggcgaaggg caagcuguuc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu ccacggcacg gugaccgucg aggugcagua cgccgggacc gacggccccu     1620

gcaagauccc cgugcagaug gcggucgaca ugcagacccu gacgccggug ggccgccuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggacaagaag aucacgcacc     1800

acuggcaccg guccggcagc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc gguccugggc gacaccgccu gggacuucgg gagcgugggc gggguguuca     1920

acucccucgg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacgcugcu cgucuggcug gggcugaaca     2040

ccaagaacgg cuccaucagc cugaccugcc ucgcccucgg gggcgugaug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5676
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 2 GC

<400>  5676
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucgggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgugaacaag ugccacgucc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgugaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ugguggcggu ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gucaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc guguccaacc gcgacuucgu cgaggggaug agcggcggca      660

ccugggugga cgugguccug gagcacgggg gcugcgugac ggugauggcg caggacaagc      720

cgaccgucga caucgagcug gugaccacca cgguguccaa cauggccgag guccggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacc cugguggacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cgugcacggc ucccagcaca gcggcaugac cgucaacgac aucggguacg     1080

agaccgacga gaaccgcgcc aagguggagg ugacccccaa cuccccgcgg gccgaggcca     1140

cccucggcgg guucggcagc cuggggcugg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccugua cuaccugacg augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg cggacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg gcagaccgug gucgugcucg     1380

gcagccagga gggggccgug cacacggcgc uggccggcgc ccuggaggcc gagauggacg     1440

gggcgaaggg caagcuguuc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

gccugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu ccacggcacg gugaccgucg aggugcagua cgccgggacc gacggccccu     1620

gcaagauccc cgugcagaug gcggucgaca ugcagacccu gacgccggug ggccggcuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggacaagaag aucacgcacc     1800

acuggcaccg cuccggcagc accaucggga aggccuucga ggccaccgug cggggcgcca     1860

agcgcauggc gguccugggc gacaccgccu gggacuucgg gagcgugggc gggguguuca     1920

acucccucgg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacgcugcu cgucuggcug gggcugaaca     2040

ccaagaacgg cuccaucagc cugaccugcc ucgcccucgg gggcgugaug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5677
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 2 GC

<400>  5677
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cguggggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgugc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aagguggaga      480

acuggaucuu ccgcaacccc ggcuucaccc ugguggccgu cgcgaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc gucuccaacc gcgacuucgu ggaggggaug agcggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

cgaccgugga caucgagcug gugaccacga ccgucuccaa cauggcggag gugcggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacg cuggucgacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacc ugcgccaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac gagaaccgcg     1080

cgaaggugga ggucaccccc aacuccccgc gggccgaggc cacccucggg ggcuucggga     1140

gccugggccu ggacugcgag ccccgcaccg ggcucgacuu cuccgaccug uacuaccuga     1200

ccaugaacaa caagcacugg cucgugcaca aggagugguu ccacgacauc ccccugcccu     1260

ggcacgccgg cgccgacacg ggcaccccgc acuggaacaa caaggaggcg cugguggagu     1320

ucaaggacgc ccacgccaag cggcagaccg ucguggugcu cgggagccag gagggcgccg     1380

uccacaccgc ccuggcgggg gcccuggagg ccgagaugga cggcgccaag gggcgccucu     1440

ucuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag ggcgugagcu     1500

acucccugug cacggcggcc uucaccuuca ccaaggugcc cgccgagacc cuccacggga     1560

ccgucacggu ggaggugcag uacgccggca ccgacgggcc cugcaagguc cccgcccaga     1620

uggcggugga caugcagacc cugaccccgg ugggccgccu gaucacggcc aaccccguca     1680

ucaccgagag caccgagaac uccaagauga ugcucgagcu ggacccgccc uucggcgaca     1740

gcuacaucgu gaucggggug ggcgacaaga agaucaccca ccacuggcac cgguccggga     1800

gcacgaucgg caaggccuuc gaggccaccg uccgcggggc caagcggaug gcggugcugg     1860

gcgacaccgc cugggacuuc ggcagcgucg ggggcguguu caacucccuc gggaagggca     1920

uccaccagau cuucggggcc gccuucaaga gccuguucgg cggcaugucc ugguucagcc     1980

agauccugau cgggacccug cucguguggc ugggccugaa cacgaagaac ggguccauca     2040

gccugaccug ccucgcccug ggcgggguga ugaucuuccu cuccaccgcg gucagcgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  5678
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 2 GC

<400>  5678
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucgggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgugaacaag ugccacgucc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgugaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ugguggcggu ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gucaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc guguccaacc gcgacuucgu cgaggggaug agcggcggca      660

ccugggugga cgugguccug gagcacgggg gcugcgugac ggugauggcg caggacaagc      720

cgaccgucga caucgagcug gugaccacca cgguguccaa cauggccgag guccggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacc cugguggacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cgugcacggc ucccagcaca gcggcaugau cggguacgag accgacgaga     1080

accgcgccaa ggucgaggug acccccaacu ccccgcgggc cgaggccacc cucggcgggu     1140

ucggcagccu ggggcuggac ugcgagcccc gcaccggccu cgacuucucc gaccuguacu     1200

accugaccau gaacaacaag cacuggcucg ugcacaagga gugguuccac gacauccccc     1260

ugcccuggca cgccggcgcg gacacgggga ccccgcacug gaacaacaag gaggcccugg     1320

ucgaguucaa ggacgcccac gccaagcggc agaccguggu gguccucggc agccaggagg     1380

gggccgugca caccgcgcug gccggcgccc uggaggccga gauggacggg gcgaagggca     1440

agcuguucuc cggccaccug aagugccgcc ugaagaugga caagcuccgc cugaaggggg     1500

ugagcuacuc ccugugcacg gccgccuuca ccuucaccaa gguccccgcc gagacccucc     1560

acggcaccgu gacgguggag guccaguacg ccgggaccga cggccccugc aagauccccg     1620

ugcagauggc gguggacaug cagacccuga ccccggucgg ccggcugauc acggccaacc     1680

ccgugaucac cgagagcacc gagaacucca agaugaugcu cgagcuggac ccgcccuucg     1740

gggacagcua caucgugauc ggcgucgggg acaagaagau cacccaccac uggcaccgcu     1800

ccggcagcac gaucgggaag gccuucgagg ccaccgugcg gggcgccaag cgcauggcgg     1860

ugcugggcga caccgccugg gacuucggga gcgucggcgg gguguucaac ucccucggca     1920

aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggg auguccuggu     1980

ucagccagau ccugaucggc acccugcucg uguggcuggg gcugaacacg aagaacggcu     2040

ccaucagccu gaccugccuc gcccucgggg gcgucaugau cuuccucucc accgcgguga     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5679
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 2 GC

<400>  5679
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg ccgggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5680
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 2 GC

<400>  5680
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggccuggccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccuccugauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcuc gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccucuucg ggaagggcag ccuggugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcucuc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcugg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcucgaggcc gagauggacg     1440

gggccaaggg ccgccugagc uccggccacc ugaagugccg gcucaagaug gacaagcugc     1500

gccugaaggg ggugagcuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu gcacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuca     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cuggagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuga     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaaca     2040

cgaagaacgg cuccaucagc cugaugugcc ucgcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5681
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 2 GC

<400>  5681
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acugguaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5682
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 2 GC

<400>  5682
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca cccagacccu gacgcccgug ggccggcuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg cuccggcagc accaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacgcuccu gauguggcug gggcucaaca     2040

ccaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5683
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 2 GC

<400>  5683
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag auccgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgugug caagcgcacc cuggucgacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cgugcacggg agccagcacu ccggcaugau cgucaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccgug gucgugcucg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggugg agguccagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccguggaca ugcagacccu gacccccguc ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg gagcgucggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5684
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 2 GC

<400>  5684
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcu ggcccagaug gccgucgaca ugcagacccu gaccccggug ggccggcuca     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cuggagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuga     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaaca     2040

cgaagaacgg cuccaucagc cugaugugcc ucgcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5685
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 2 GC

<400>  5685
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgacccugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc acgugggugg uguacgggac cugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cgcgcagcca gaccuggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggucga cguggugcug gagcacgggg gcugcgucac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacga ccgugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacg cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggc agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agacggacga gaaccgggcc aaggucgaga ucaccccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacg augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacaccgg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacgcccgug ggccggcuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg cuccggcagc accaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacgcuccu gauguggcug gggcucaaca     2040

ccaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5686
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 2 GC

<400>  5686
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgucgu gagcgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5687
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 2 GC

<400>  5687
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc gcgugggugg uguacgggac cugccaccac aagaagggcg      360

aggcccggcg cucccggcgc gccgucacgc uccccagcca cuccacccgg aagcugcaga      420

cccgccccca gaccuggcug gagagccggg aguacacgaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccg ggcuucgccc uggcggccgc cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggucaugau ccugcucauc gcgcccgccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac cgugauggcc caggacaagc      720

ccacggugga caucgagcug gucaccacca ccguguccaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg cguccgacag ccggugcccc acgcaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacc ugcgccaagu      960

ucgccugcag caagaagaug acggggaagu ccauccagcc ggagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacccccaa cagcccccgc gcggaggcca     1140

cgcucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgcgc acgccaagcg ccagaccguc guggugcucg     1380

gcagccagga gggggccguc cacaccgccc uggcgggcgc ccuggaggcc gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg gguguccuac agccugugca ccgccgcguu cacguucacc aagauccccg     1560

ccgagacccu ccacggcacc gugaccgucg aggugcagua cgccgggacg gacggcccgu     1620

gcaaggugcc cgcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucaccgcgaa ccccgugauc acggagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cagcggcucc accaucggga aggccuucga ggccacggug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgcgu gggacuucgg gagcgugggc ggggcccuca     1920

acagccuggg caaggggauc caccagaucu ucggcgccgc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

ccaagaacgg cagcaucucc cugaugugcc uggcgcucgg gggcgugcug aucuuccuga     2100

gcacggccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5688
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 2 GC

<400>  5688
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagcaagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5689
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 2 GC

<400>  5689
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac ccgccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acagcauccg gugcaucggc guguccaacc gcgacuucgu ggaggggaug agcggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cgguguccaa cauggccgag gugcggagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccgcugcccc acccaggggg      840

aggcguaccu cgacaagcag agcgacaccc aguacgucug caagcggacc cugguggacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgccaagu      960

ucgccugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacgccgaa cagcccccgg gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcagccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucucc agcggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg gguguccuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccgccuga     1680

ucacggccaa ccccgugauc accgagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg gagcggcucc acgaucggga aggcguucga ggccaccgug cgcggcgcca     1860

agcggauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acagccuggg caaggggauc caccagaucu ucggcgccgc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cagcaucucc cugaugugcc uggcccucgg gggcgugcug aucuuccuga     2100

gcaccgcggu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5690
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 2 GC

<400>  5690
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcacccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg gguguccuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacgcccgug ggccggcuga     1680

ucaccgccaa ccccgugauc accgagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguca ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg cagcggcucc accaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acagccuggg caaggggauc caccagaucu ucggcgccgc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacgcuccu gauguggcug gggcucaaca     2040

ccaagaacgg cagcaucucc cugaugugcc uggcccucgg gggcgugcug aucuuccuga     2100

gcaccgcggu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5691
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 2 GC

<400>  5691
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acucccugcg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcuc gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccucuucg ggaagggcag ccuggugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcucuc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcugg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcucgaggcc gagauggacg     1440

gggccaaggg ccgccugagc uccggccacc ugaagugccg gcucaagaug gacaagcugc     1500

gccugaaggg ggugagcuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu gcacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuca     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cuggagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuga     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaaca     2040

cgaagaacgg cuccaucagc cugaugugcc ucgcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5692
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 2 GC

<400>  5692
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcaucgaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac ggucauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca cggucagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgugug caagcgcacc cuggucgacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cgugcacggg agccagcacu ccggcaugau cgucaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccgug gucgugcucg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggugg agguccagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccguggaca ugcagacccu gacccccguc ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg gagcgucggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5693
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 2 GC

<400>  5693
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggauccu gcggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccuccugauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcuc gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccucuucg ggaagggcag ccuggugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcucuc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcugg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcucgaggcc gagauggacg     1440

gggccaaggg ccgccugagc uccggccacc ugaagugccg gcucaagaug gacaagcugc     1500

gccugaaggg ggugagcuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu gcacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuca     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cuggagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcaucgg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cgugcucggc gacaccgccu gggacuucgg gagcgucggc ggggcgcuga     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaaca     2040

cgaagaacgg cuccaucagc cugaugugcc ucgcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5694
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 2 GC

<400>  5694
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucagcacca ccguguccaa cauggccgag gugcgcagcu      780

acugcuacga ggccacgauc uccgacaucg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccgcc gucgugcucg     1380

gcucccagga gggggccgug cacaccgccc uggcgggcgc ccuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggucagcuac ucccugugca ccgccgcguu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gugacggugg agguccagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccguggaca ugcagacccu gacccccguc ggccggcuga     1680

ucacggcgaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggccuucga ggccaccgug cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgcgu gggacuucgg gagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcgcucgg gggcgugcug aucuuccugu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5695
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 2 GC

<400>  5695
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg agcugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccguggaca ugcagacccu gacccccguc ggccggcuca     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cuggagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cgugcucggc gacaccgccu gggacuucgg gagcgucggc ggggcgcuga     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaaca     2040

cgaagaacgg cuccaucagc cugaugugcc ucgcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5696
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 2 GC

<400>  5696
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ucgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc accaucggga aggcguucga ggccacggug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

ccaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccacggcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5697
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 2 GC

<400>  5697
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggugaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucacggugg aggugcagua cgcggggacc gacggccccu     1620

gcaagguccc ggcccagaug gccguggaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgucauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggccuucga ggcgaccguc cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg gagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgcggc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc uucaugugcc uggcccucgg gggcguccug aucuuccugu     2100

ccaccgccgu gagcgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5698
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 2 GC

<400>  5698
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg caagcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccgccuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg guccggcagc acgaucggga aggcguucga ggccaccgug cgcggcgcca     1860

agcggauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5699
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 2 GC

<400>  5699
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca aggacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5700
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 2 GC

<400>  5700
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggaggccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5701
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 2 GC

<400>  5701
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcgg     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacaccgg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgcgc acgccaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggcgggcgc ccuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgcguu cacguucacc aagauccccg     1560

ccgagacccu ccacggcacc gugaccgucg aggugcagua cgccgggacg gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucaccgcgaa ccccgugauc acggagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc accaucggga aggccuucga ggccacggug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgcgu gggacuucgg gagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag acccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcgcucgg gggcgugcug aucuuccugu     2100

ccaccgccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5702
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 2 GC

<400>  5702
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccggggcc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucaccgcgaa ccccgugauc acggagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc accaucggga aggccuucga ggccacggug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgcgu gggacuucgg gagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

ccaagaacgg cuccaucagc cugaugugcc uggcgcucgg gggcgugcug aucuuccugu     2100

ccacggccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5703
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 2 GC

<400>  5703
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gcccuggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

cgaccgugga caucgagcuc gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccucuucg ggaagggcag ccuggugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcucuc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgcccaa cagcccgcgc gccgaggcga     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacacggg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcugg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcucgaggcc gagauggacg     1440

gggccaaggg ccgccugagc uccggccacc ugaagugccg gcucaagaug gacaagcugc     1500

gccugaaggg ggugagcuac ucccucugca cggccgccuu caccuucacc aagauccccg     1560

cggagacccu gcacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc cgcccagaug gccgucgaca ugcagacccu gaccccggug ggccggcuca     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cuggagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuga     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaaca     2040

cgaagaacgg cuccaucagc cugaugugcc ucgcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5704
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 2 GC

<400>  5704
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ucagcgacag ccggugcccc acccaggggg      840

aggccuaccu cgacaagcag uccgacaccc aguacgugug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgucacg ugcgcgaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cgugcacggg agccagcacu ccggcaugau cgucaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucacgccgaa cagcccccgc gccgaggcca     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgcgggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu ggucgaguuc aaggacgccc acgccaagcg ccagaccgug gugguccucg     1380

gcucccagga gggggcggug cacaccgccc uggccggcgc ccuggaggcg gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucacggugg aggugcagua cgcggggacc gacggccccu     1620

gcaagguccc ggcccagaug gccguggaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgucauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggccuucga ggcgaccguc cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg gagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgcggc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcguccug aucuuccugu     2100

ccaccgccgu gagcgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5705
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 2 GC

<400>  5705
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc aucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac ggucauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca cggucagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgugug caagcgcacc cuggucgacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cgugcacggg agccagcacu ccggcaugau cgucaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcauc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccgug gucgugcucg     1380

gcagccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggucuccuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggugg agguccagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccguggaca ugcagacccu gacccccguc ggccggcuga     1680

ucacggccaa ccccgugauc accgagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg cagcggcucc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg gagcgucggc ggggcgcuca     1920

acagccuggg caaggggauc caccagaucu ucggcgccgc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cagcaucucc cugaugugcc uggcccucgg gggcgugcug aucuuccuga     2100

gcaccgcggu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5706
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 2 GC

<400>  5706
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac acgugcgacg      240

ccaccauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc acgugggugg uguacgggac cugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccauccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggc agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaacg     2040

ccaagaacgg cuccaucagc cugaugugcc uggcgcucgg gggcgugcug aucuuccugu     2100

ccacggccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5707
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 2 GC

<400>  5707
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcagcg gucccgccgg gccgucaccc uccccagcca cuccacccgc aagcugcaga      420

cccggagcca gacguggcug gagucccgcg aguacaccaa gcaccucauc cggguggaga      480

acuggaucuu ccgcaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg gugcaucggc gugagcaacc gcgacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgguccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccgcugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcggacc cugguggacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgga     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacgccgaa cagcccccgg gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccgcaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg gcagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccgccuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg guccggcagc acgaucggga aggcguucga ggccaccgug cgcggcgcca     1860

agcggauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5708
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 2 GC

<400>  5708
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcagg      420

cccgcagcca gaccuggcug gagucccggg aguacacgaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggcggccgc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gcgccggccu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac cgugauggcc caggacaagc      720

ccacggugga caucgagcug gucaccacca ccgugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg cgagcgacag ccggugcccc acgcaggggg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacc ugcgccaagu      960

ucgccugcuc caagaagaug acgggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucaccccgaa cagcccccgc gcggaggcca     1140

cgcucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacaccgg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgcgc acgccaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggcgggcgc ccuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgcguu cacguucacc aagauccccg     1560

ccgagacccu ccacggcacc gugaccgucg aggugcagua cgccgggacg gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucaccgcgaa ccccgugauc acggagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc accaucggga aggccuucga ggccacggug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgcgu gggacuucgg gagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

ccaagaacgg cuccaucagc cugaugugcc uggcgcucgg gggcgugcug aucuuccugu     2100

ccacggccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5709
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 2 GC

<400>  5709
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg ggaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cgguguccaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag agcgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgccaagu      960

ucgccugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcagccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg gguguccuac agccugugca ccgccgccuu caccuucucc aagauccccg     1560

cggagacccu ccacggcacc gugaccgagg agguccagua cgccgggacg gacggccccu     1620

gcaaggugcc ggcccagaug gccguggaca ugcagacccu gacccccguc ggccggcuga     1680

ucaccgccaa ccccgugauc acggagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg cagcggcucc accaucggga aggcguucga ggccacggug cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg gagcgucggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

ccaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccacggcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5710
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 2 GC

<400>  5710
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg ggaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cgguguccaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag agcgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgccaagu      960

ucgccugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacacgggcg ccgacaccgg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc guggugcucg     1380

gcagccagga gggggcgguc cacaccgccc uggccggcgc ccuggaggcg gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg gguguccuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacg gugaccgucg aggugcagua cgcggggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacgcccgug ggccggcuga     1680

ucaccgccaa ccccgugauc accgagggga ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggcgacagc uacaucguca ucgggguggg cgagaagaag aucacgcacc     1800

acuggcaccg cuccgggagc accaucggca aggccuucga ggcgaccgug cggggcgcca     1860

agcgcauggc cguccugggg gacaccgccu gggacuucgg cagcgucggg ggcgugcuca     1920

acucccuggg gaagggcauc caccagaucu ucggcgccgc guucaagagc cuguucgggg     1980

gcauguccug guucagccag auccugaucg ggacgcuccu gauguggcug ggccucaaca     2040

ccaagaacgg guccaucagc cugaugugcc uggcccucgg cggcgugcug aucuuccugu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5711
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 2 GC

<400>  5711
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg ggaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cgguguccaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag agcgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgccaagu      960

ucgccugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcagccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg gguguccuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cagcggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg guccgugggc ggggcgcuca     1920

acagccuggg caaggggauc caccagaucu ucggcgccgc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cagcaucucc cugaugugcc uggcccucgg gggcgugcug aucuuccuga     2100

gcaccgcggu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5712
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 2 GC

<400>  5712
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cguggaggug acccgccggg      120

gcaacgcgua cuacauguac cuggaccgcu ccgacgccgg ggaggccauc agcuucccca      180

ccaccauggg caugaacaag ugcuacaucc agaucaugga ccucgggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg ucuacggcac gugccaccac aagaaggggg      360

aggcccggcg cucccggcgc gcggugaccc ugcccagcca cuccacccgg aagcuccaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccugauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc ucgccgccgc ggccaucgcc uggcugcugg      540

ggagcuccac cagccagaag gucaucuacc ucgugaugau ccugcugauc gccccggccu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu cgaggggaug uccggcggca      660

ccugggugga cgugguccuc gagcacgggg gcugcgugac ggugauggcg caggacaagc      720

ccaccgucga caucgagcug gugaccacca cggugagcaa cauggccgag guccgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggccuaccu ggacaagcag uccgacaccc aguacgugug caagcgcacc cucguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccuggucacg ugcgcgaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccuc gaguaccgca     1020

ucaugcuguc cgugcacggg agccagcacu ccggcaugau cgucaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucacgccgaa cagcccccgc gccgaggcca     1140

cccugggcgg guucggcucc cucgggcugg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccucua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccucccgugg cacgcgggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu ggucgaguuc aaggacgccc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggcggug cacaccgccc ucgccggcgc ccuggaggcg gagauggacg     1440

gggccaaggg ccgccugagc uccggccacc ucaagugccg gcugaagaug gacaagcugc     1500

gccucaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gucacggugg aggugcagua cgcggggacc gacggccccu     1620

gcaagguccc ggcccagaug gccguggaca ugcagacccu cacccccgug ggccggcuga     1680

ucacggccaa ccccgucauc accgagagca ccgagaacuc caagaugaug cuggagcucg     1740

acccgcccuu cggggacagc uacaucguga ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggccuucga ggcgaccguc cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg gagcgugggc ggggcccuga     1920

acucccucgg caaggggauc caccagaucu ucggcgcggc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccugcu cgucuggcug gggcugaaca     2040

cgaagaacgg cuccaucagc cucaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgccgu gagcgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5713
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 2 GC

<400>  5713
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg ggaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cgguguccaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag agcgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgccaagu      960

ucgccugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcagccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg gguguccuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cagcggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg guccgugggc ggggcgcuca     1920

acagccuggg caaggggauc caccagaucu ucggcgccgc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cagcaucucc cuggugugcc uggcccucgg gggcguccug aucuuccuga     2100

gcaccgcggu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5714
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 2 GC

<400>  5714
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg ggaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acagcauccg cugcaucggc gugaacaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cuggugugcc uggcccucgg gggcguccug aucuuccugu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5715
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 2 GC

<400>  5715
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cguggaggug acccgccggg      120

gcuccgcgua cuacauguac cuggaccgca gcgacgccgg ggaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggugg ucuacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gcggugaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgccgc ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gucaucuacc uggugaugau ccugcucauc gccccggccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu cgaggggaug agcggcggca      660

ccugggugga cgugguccug gagcacgggg gcugcgugac ggugauggcg caggacaagc      720

ccgccgucga caucgagcug gugaccacca ccguguccaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acgcaggggg      840

aggcguaccu cgacaagcag agcgacaccc aguacgugug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacc ugcgccaagu      960

ucgccugcag caagaagaug acggggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgucaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucaccccgaa cagcccccgc gccgaggcga     1140

cgcucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacaccgg gacgccccac uggaacaaca     1320

aggaggcccu ggucgaguuc aaggacgccc acgcgaagcg ccagaccgug gugguccucg     1380

gcagccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg gguguccuac agccugugca ccgccgccuu cacguucacc aagauccccg     1560

cggagacccu ccacggcacc gucaccgugg aggugcagua cgccgggacg gacggccccu     1620

gcaagguccc ggcccagaug gccguggaca ugcagacccu gacccccgug ggccggcuga     1680

ucaccgccaa ccccgucauc acggagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguga ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cagcggcagc accaucggga aggcguucga ggccacgguc cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg guccgugggc ggggcgcuca     1920

acagccuggg caaggggauc caccagaucu ucggcgccgc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu ggucuggcug gggcucaaca     2040

ccaagaacgg cagcaucucc cugacgugcc uggcccucgg gggcgugcug aucuuccuga     2100

gcaccgcggu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5716
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 2 GC

<400>  5716
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cguggaggug acccgccggg      120

gcuccgcgua cuacauguac cuggaccgca gcgacgccgg ggaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggugg ucuacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gcggugaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgccgc ggucaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggugaugau ccugcucauc gccccggccu      600

acagcauccg cugcaucggc gucuccaacc gggacuucgu ggaggggaug agcggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac ggucauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca cggucuccaa cauggcggag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcgcacc cuggucgacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgccaagu      960

ucgcgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucacgccgaa cagcccccgc gccgaggcca     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg cggacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccgug gucgugcucg     1380

gcagccagga gggggccgug cacaccgcgc uggccggcgc ccuggaggcc gagauggacg     1440

gggcgaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggucuccuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gugacggugg agguccagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcgcagaug gccguggaca ugcagacccu gacccccguc ggccggcuga     1680

ucacggccaa ccccgugauc accgagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg cagcggcagc acgaucggga aggccuucga ggccaccgug cggggcgcga     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg guccgucggc ggggcccuca     1920

acagccuggg caaggggauc caccagaucu ucggcgccgc guucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu gguguggcug gggcucaaca     2040

cgaagaacgg cagcaucucc cugaccugcc uggcccucgg gggcgugcug aucuuccuga     2100

gcaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5717
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 2 GC

<400>  5717
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cguggaggug acccgccggg      120

gcuccgcgua cuacauguac cuggaccgca gcgacgccgg ggaggccauc uccuucccca      180

ccacccucgg cgucaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gcggugaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgccgc ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggugaugau ccugcucauc gccccggccu      600

acagcauccg cugcaucggc gucuccaacc gggacuucgu ggaggggaug agcggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac ggucauggcg caggacaagc      720

ccaccgugga caucgagcug gugaccacca cggucuccaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcgcacc cuggucgacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgcgaagu      960

ucgccugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucacgccgaa cagcccccgc gccgaggcca     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugccgugg cacgcgggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccgug gucgugcucg     1380

gcagccagga gggggcggug cacaccgccc uggccggcgc ccuggaggcg gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggucuccuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gugacggugg agguccagua cgcggggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccguggaca ugcagacccu gacccccguc ggccggcuga     1680

ucacggccaa ccccgugauc accgagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguga ucggcgucgg ggacaagaag aucacccacc     1800

acugguaccg cagcggcagc acgaucggga aggccuucga ggcgaccgug cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg guccgucggc ggggcccuca     1920

acagccuggg caaggggauc caccagaucu ucggcgcggc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu gguguggcug gggcucaaca     2040

cgaagaacgg cagcaucucc cugaccugcc uggcccucgg gggcgugcug aucuuccuga     2100

gcaccgccgu cuccgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5718
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 2 GC

<400>  5718
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cguggaggug acccgccggg      120

gcuccgcgua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucccca      180

ccacccucgg cgucaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgaugag cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gcggugaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgccgc ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggugaugau ccugcucauc gccccggccu      600

acagcauccg cugcaucggc gucuccaacc gggacuucgu ggaggggaug agcggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac ggucauggcg caggacaagc      720

ccaccgugga caucgagcug gugaccacca cggucuccaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcgcacc cuggucgacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgcgaagu      960

ucgccugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac aucgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucaccccgaa cagcccccgc gccgaggcca     1140

cgcucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugccgugg cacgcgggcg ccgacaccgg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccgug gucgugcucg     1380

gcagccagga gggggcggug cacaccgccc uggccggcgc ccuggaggcg gagauggacg     1440

gggccaaggg ccgccucucc agcggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggucuccuac agccugugca ccgccgccuu cacguucacc aagauccccg     1560

ccgagacccu ccacggcacc gugaccgugg agguccagua cgcggggacg gacggccccu     1620

gcaaggugcc ggcccagaug gccguggaca ugcagacccu gacccccguc ggccggcuga     1680

ucaccgccaa ccccgugauc acggagucca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggggacucc uacaucguga ucggcgucgg ggacaagaag aucacccacc     1800

acuggcaccg cagcggcagc accaucggga aggccuucga ggcgacggug cggggcgcca     1860

agcgcauggc cgugcugggc gacaccgccu gggacuucgg guccgucggc ggggcccuca     1920

acagccuggg caaggggauc caccagaucu ucggcgcggc cuucaagucc cuguucggcg     1980

ggaugagcug guucucccag auccugaucg gcacccuccu gguguggcug gggcucaaca     2040

ccaagaacgg cagcaucucc cugacgugcc uggcccucgg gggcgugcug aucuuccuga     2100

gcaccgccgu cuccgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5719
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 2 GC

<400>  5719
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cguggggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgugc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aagguggaga      480

acuggaucuu ccgcaacccc ggcuucaccc ugguggccgu cgcgaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc gucuccaacc gcgacuucgu ggaggggaug agcggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

cgaccgugga caucgagcug gugaccacga ccgucuccaa cauggcggag gugcggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacg cuggucgacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacc ugcgccaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgcgcg aagguggagg ucacccccaa cuccccgcgg gccgaggcca     1140

cccucggcgg guucggcagc cuggggcugg acugcgagcc ccgcacgggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcgcu gguggaguuc aaggacgccc acgccaagcg gcagacgguc guggugcucg     1380

gcagccagga gggggccguc cacaccgccc uggcgggcgc ccuggaggcc gagauggacg     1440

gggccaaggg ccgccucuuc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgcggccuu caccuucacc aaggugcccg     1560

ccgagacgcu ccacggcacc gucaccgugg aggugcagua cgccgggacc gacggccccu     1620

gcaagguccc cgcccagaug gcgguggaca ugcagacgcu gaccccggug ggccgccuga     1680

ucaccgccaa ccccgucauc accgagagca cggagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcguggg ggacaagaag aucacccacc     1800

acuggcaccg guccggcagc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc ggugcugggc gacacggccu gggacuucgg gagcgugggc ggggucuuca     1920

acucccucgg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccugcu cguguggcug gggcugaaca     2040

ccaagaacgg cuccaucagc cugaccugcc uggcccucgg gggcgugaug aucuuccucu     2100

ccacggcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5720
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 2 GC

<400>  5720
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucgggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccaaccucgg cgugaacaag ugccacgucc agaucaugga ccuggggcac augugcgacg      240

cgaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa cacgaccucc accugggucg uguacgggac cugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgugacgc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gaccuggcug gagagccggg aguacacgaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc uggcggccgu ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggucaugau ccugcucauc gcccccgcgu      600

acagcauccg gugcaucggc guguccaacc gcgacuucgu ggaggggaug agcggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac cgugauggcc caggacaagc      720

cgacggugga caucgagcug gucaccacca ccguguccaa cauggccgag gugcggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acgcaggggg      840

aggcguaccu cgacaagcag agcgacaccc aguacgucug caagcggacc cugguggacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacc ugcgccaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac aucgggcacg     1080

agaccgacga gaaccgcgcc aaggucgagg ugacccccaa cuccccgcgg gccgaggcca     1140

cgcucggcgg guucggcagc cuggggcugg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgcgggcg ccgacaccgg gacgccgcac uggaacaaca     1320

aggaggcccu ggucgaguuc aaggacgccc acgccaagcg gcagaccgug gugguccucg     1380

gcagccagga gggggcggug cacaccgccc uggccggcgc ccuggaggcg gagauggacg     1440

gggccaaggg ccgccucuuc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu cacguucacc aagguccccg     1560

ccgagacccu ccacggcacc gugaccgugg agguccagua cgcggggacg gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gaccccgguc ggccgccuga     1680

ucaccgccaa ccccgugauc acggagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcgucgg ggacaagaag aucacccacc     1800

acuggcaccg guccggcagc accaucggga aggccuucga ggcgacggug cgcggcgcca     1860

agcggauggc cgugcugggc gacaccgccu gggacuucgg gagcgucggc gggguguuca     1920

acucccucgg caagggggug caccagaucu ucggcgccgc guucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccugcu cgucuggcug gggcugaaca     2040

ccaagaacgg cuccaucagc cucacgugcc uggcccuggg cggcgugaug aucuuccucu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5721
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 2 GC

<400>  5721
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucgggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgugaacaag ugccacgucc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccucc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgugaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ugguggcggu ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gucaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc guguccaacc gcgacuucgu cgaggggaug agcggcggca      660

ccugggugga cgugguccug gagcacgggg gcugcgugac ggugauggcg caggacaagc      720

cgaccgucga caucgagcug gugaccacca cgguguccaa cauggccgag guccggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacc cugguggacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cgugcacggc ucccagcaca gcggcaugau cgucaacgac aucgggcacg     1080

agaccgacga gaaccgcgcc aagguggagg ugacccccaa cuccccgcgg gccgaggcca     1140

cccucggcgg guucggcagc cuggggcugg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg cggacacggg gaccccgcac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg gcagaccgug gucgugcucg     1380

gcagccagga gggggccgug cacaccgcgc uggccggcgc ccuggaggcc gagauggacg     1440

gggcgaaggg ccgccucuuc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca cggccgccuu caccuucacc aaggugcccg     1560

ccgagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc cgcgcagaug gccgucgaca ugcagacccu gaccccggug ggccgccuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggacaagaag aucacccacc     1800

acuggcaccg guccggcagc acgaucggga aggccuucga ggccaccgug cgcggcgcga     1860

agcggauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc gggguguuca     1920

acucccucgg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccugcu cgucuggcug gggcugaaca     2040

cgaagaacgg cuccaucagc cugaccugcc uggcgcucgg gggcgugaug aucuuccucu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5722
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 2 GC

<400>  5722
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucgggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccaaccucgg cgugaacaag ugccacgucc agaucaugga ccuggggcac augugcgacg      240

cgaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa cacgaccucc accugggucg uguacgggac cugccaccac aagaagggcg      360

aggcccggcg cagccggcgc gccgugacgc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gaccuggcug gagagccggg aguacacgaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc uggcggccgu ggccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggucaugau ccugcucauc gcccccgcgu      600

acagcauccg gugcaucggc guguccaacc gcgacuucgu ggaggggaug agcggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac cgugauggcc caggacaagc      720

cgacggugga caucgagcug gucaccacca ccguguccaa cauggccgag gugcggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acgcaggggg      840

aggcguaccu cgacaagcag agcgacaccc aguacgucug caagcggacc cugguggacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacc ugcgccaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac gagaaccgcg     1080

ccaaggucga ggugaccccc aacuccccgc gggccgaggc cacccucggg ggcuucggga     1140

gccugggccu ggacugcgag ccccgcacgg ggcucgacuu cuccgaccug uacuaccuga     1200

ccaugaacaa caagcacugg cucgugcaca aggagugguu ccacgacauc ccccugcccu     1260

ggcacgcggg cgccgacacc ggcaccccgc acuggaacaa caaggaggcc cuggucgagu     1320

ucaaggacgc ccacgccaag cggcagacgg uggugguccu cgggagccag gagggcgcgg     1380

ugcacaccgc ccuggccggg gcccuggagg cggagaugga cggcgccaag gggcgccucu     1440

ucuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag ggcgugagcu     1500

acucccugug caccgccgcc uucaccuuca cgaagguccc cgccgagacc cuccacggga     1560

ccgugaccgu ggagguccag uacgcgggca ccgacgggcc cugcaaggug cccgcccaga     1620

uggccgugga caugcagacg cugaccccgg ucggccgccu gaucaccgcc aaccccguga     1680

ucaccgagag cacggagaac uccaagauga ugcucgagcu ggacccgccc uucggcgaca     1740

gcuacaucgu gaucgggguc ggcgacaaga agaucaccca ccacuggcac cgguccggga     1800

gcaccaucgg caaggccuuc gaggcgaccg ugcgcggggc caagcggaug gccgugcugg     1860

gcgacacggc cugggacuuc ggcagcgucg ggggcguguu caacucccuc gggaagggca     1920

uccaccagau cuucggggcc gcguucaaga gccuguucgg cggcaugucc ugguucagcc     1980

agauccugau cgggacccug cucguguggc ugggccugaa caccaagaac ggguccauca     2040

gccugaccug ccucgcccug ggcgggguca ugaucuuccu cuccacggcc gugagcgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  5723
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 2 GC

<400>  5723
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cguggggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgugc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aagguggaga      480

acuggaucuu ccgcaacccc ggcuucgccc ugguggcggu cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc gucuccaacc gcgacuucgu ggaggggaug agcggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac ggucauggcg caggacaagc      720

cgaccgugga caucgagcug gugaccacca cggucuccaa cauggccgag gugcggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacc cuggucgacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgcgcc aagguggagg ucacccccaa cuccccgcgg gccgaggcca     1140

cccucggcgg guucggcagc cuggggcugg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccugua cuaccugacg augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg cggacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg gcagaccguc guggugcucg     1380

gcagccagga gggggccguc cacacggcgc uggccggcgc ccuggaggcc gagauggacg     1440

gggcgaaggg ccgccucuuc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggccccu     1620

gcaagguccc cgcgcagaug gccguggaca ugcagacccu gacgccggug ggccgccuga     1680

ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcguggg ggacaagaag aucacgcacc     1800

acuggcaccg guccggcagc accaucggga aggccuucga ggccaccguc cgcggcgcga     1860

agcggauggc cgugcugggc gacaccgccu gggacuucgg gagcgugggc ggggucuuca     1920

acucccucgg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacgcugcu cguguggcug gggcugaaca     2040

ccaagaacgg cuccaucagc cugaccugcc uggcgcucgg gggcgugaug aucuuccucu     2100

ccaccgccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5724
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 2 GC

<400>  5724
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cguggggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgugc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccagcac aagaaggggg      360

aggcccggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aagguggaga      480

acuggaucuu ccgcaacccc ggcuucgccc ugguggcggu cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc gucuccaacc gcgacuucgu ggaggggaug agcggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac ggucauggcg caggacaagc      720

cgaccgugga caucgagcug gugaccacca cggucuccaa cauggccgag gugcggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacc cuggucgacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac aucgggcacg     1080

agaccgacga gaaccgcgcc aagguggagg ucacccccaa cuccccgcgg gccgaggcca     1140

cccucggcgg guucggcagc cuggggcugg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg cggacacggg gaccccgcac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagcg gcagaccguc guggugcucg     1380

gcagccagga gggggccguc cacaccgcgc uggccggcgc ccuggaggcc gagauggacg     1440

gggcgaaggg ccgccucuuc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca cggccgugug caccgccgcc aagguccccg     1560

ccgagacccu ccacggcacc gugaccgugg agguccagua cgcggggacg gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gaccccgguc ggccgccuga     1680

ucaccgccaa ccccgugauc acggagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcgucgg ggacaagaag aucacccacc     1800

acuggcaccg guccggcagc accaucggga aggccuucga ggcgacggug cgcggcgcca     1860

agcggauggc cgugcugggc gacaccgccu gggacuucgg gagcgucggc gggguguuca     1920

acucccucgg caaggggauc caccagaucu ucggcgccgc guucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccugcu cguguggcug gggcugaaca     2040

ccaagaacgg cuccaucagc cucacgugcc uggcccucgg gggcgucaug aucuuccugu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5725
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 2 GC

<400>  5725
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cguggggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgugc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcccggcg cagccggcgc gccgucaccc uccccuccca cagcacccgg aagcugcaga      420

cccgcuccca gacguggcug gagagccggg aguacaccaa gcaccucauc aagguggaga      480

acuggaucuu ccgcaacccc ggcuucgccc ugguggcggu cgccaucgcc uggcugcucg      540

gguccagcac cucccagaag gugaucuacc uggugaugau ccugcucauc gcccccgccu      600

acagcauccg gugcaucggc gucuccaacc gcgacuucgu ggaggggaug agcggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac ggucauggcg caggacaagc      720

cgaccgugga caucgagcug gugaccacca cggucuccaa cauggccgag gugcggagcu      780

acugcuacga ggccuccauc agcgacaugg ccagcgacuc ccgcugcccc acccaggggg      840

aggccuaccu cgacaagcag agcgacaccc aguacgugug caagcggacc cuggucgacc      900

gcggcugggg caacgggugc ggccuguucg ggaagggcuc ccucgugacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccug gaguaccgga     1020

ucaugcugag cguccacggc ucccagcaca gcggcaugau cgugaacgac gagaaccgcg     1080

ccaaggugga ggucaccccc aacuccccgc gggccgaggc cacccucggg ggcuucggga     1140

gccugggccu ggacugcgag ccccgcaccg ggcucgacuu cuccgaccug uacuaccuga     1200

ccaugaacaa caagcacugg cucgugcaca aggagugguu ccacgacauc ccccugcccu     1260

ggcacgccgg cgcggacacc ggcacgccgc acuggaacaa caaggaggcc cugguggagu     1320

ucaaggacgc ccacgccaag cggcagaccg ucguggugcu cgggagccag gagggcgccg     1380

uccacaccgc gcuggccggg gcccuggagg ccgagaugga cggcgcgaag gggcgccucu     1440

ucuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag ggcgugagcu     1500

acucccugug caccgccgcc uucacguuca ccaaggugcc cgccgagacc cuccacggga     1560

ccgucaccgu ggaggugcag uacgccggca cggacgggcc cugcaagguc cccgcgcaga     1620

uggccgugga caugcagacc cugaccccgg ugggccgccu gaucaccgcc aaccccguca     1680

ucacggagag caccgagaac uccaagauga ugcucgagcu ggacccgccc uucggcgaca     1740

gcuacaucgu gaucggggug ggcgacaaga agaucaccca ccacuggcac cgguccggga     1800

gcaccaucgg caaggccuuc gaggccacgg uccgcggggc gaagcggaug gccgugcugg     1860

gcgacaccgc cugggacuuc ggcagcgucg ggggcguguu caacucccuc gggaagggca     1920

uccaccagau cuucggggcc gccuucaaga gccuguucgg cggcaugucc ugguucagcc     1980

agauccugau cgggacccug cucguguggc ugggccugaa caccaagaac ggguccauca     2040

gccucacgug ccuggcgcug ggcgggguga ugaucuuccu cuccaccgcc gucagcgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  5726
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 2 GC

<400>  5726
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucgggcug cuccugacca cggccauggc cgcggagauc acccgccggg      120

gcuccgccua cuacauguac cuggaccgca gcgacgccgg gaaggccauc uccuucgcca      180

ccacccucgg cgugaacaag ugccacgucc agaucaugga ccuggggcac augugcgacg      240

cgacgaugag cuacgagugc cccaugcugg acgagggcgu ggagccggac gacguggacu      300

gcuggugcaa caccaccucc accugggucg uguacggcac gugccaccac aagaaggggg      360

agacccggcg cagccggcgc uccgugagcc uccgguacca cuacacccgc aagcugcaga      420

cccgguccca gacguggcug gagagccgcg aguacaagaa gcaccucauc auggucgaga      480

acuggaucuu ccggaacccc ggcuucgcca ucguguccgu ggccaucacc uggcugaugg      540

ggagccugac cucccagaag gucaucuacc ucgugaugau cgugcugauc guccccgccu      600

acagcaucuc cugcaucggc gugagcaacc gcgaccuggu ggaggggaug uccggcggca      660

ccugggucga cguggugcuc gagcacgggg gcugcgucac ggagauggcg caggacaagc      720

ccaccgugga caucgagcug gugaccauga ccgucagcaa cauggccgag gugcgguccu      780

acugcuacga ggccagccug agcgacaugg ccuccgccag ccgcugcccg acgcaggggg      840

agcccucccu cgacaagcag agcgacaccc aguccgugug caagcggacc cugggcgacc      900

gcggcugggg gaacggcugc gggaucuucg gcaaggggag ccuggugacc ugcuccaagu      960

ucacgugcug caagaagaug cccggcaaga gcauccagcc cgagaaccuc gaguaccgga     1020

ucaugcugcc ggugcacggc ucccagcaca gcgggaugau cgucaacgac aucggccacg     1080

agaccgacga gaaccgcgcg aagguggagg ugacccccaa cagcccccgg gccgaggcca     1140

cccucggggg cuucgggucc cugggccugg acugcgagcc ccgcacgggc cuggacuucu     1200

ccgaccucua cuaccugacc augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcuccccugg cacgccgggg ccgacaccgg cacgccccac uggaacaaca     1320

aggaggcgcu gguggaguuc aaggacgccc acgccaagcg gcagaccgug gucgugcugg     1380

ggagccagga gggcgccgug cacaccgccc ucgcgggggc ccuggaggcc gagauggacg     1440

gcgccaaggg ccgccuguuc uccgggcacc ucaagugccg ccugaagaug gacaagcugc     1500

ggcucaaggg cgucagcuac ucccugugca ccgccgcguu caccuucacg aaggugcccg     1560

ccgagacccu gcacgggacc gugaccgucg aggugcagag cgccggcacc gacggcccgu     1620

gcaaggugcc cgcccagaug gcggucgaca ugcagacgcu cacccccgug gggcgccuga     1680

ucaccgccaa ccccgugauc accgagucca cggagaacag caagaugaug cuggagcucg     1740

acccgcccuu cggcgacagc uacaucguca ucgggguggg cgacaagaag aucacccacc     1800

acuggcaccg guccgggagc accaucggca aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc gguccugggg gacacggccu gggacuucgg cuccgucggg ggcguguuca     1920

acagccuggg gaagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucgggg     1980

gcaugagcug guucucccag auccugaucg ggacccugcu ggucuggcuc ggccugaaca     2040

ccaagaacgg gagcaucucc cugaccugcc ucgcccuggg cggcgugaug aucuuccuga     2100

gcacggcggu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5727
<211>  2446
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 2 GC

<400>  5727
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcg       60

ugggcaucgu cgggcugcuc cugaccaccg ccauggccgc ggaggugacg cgccggggcu      120

ccgccuacua cauguaccug gaccgcaacg acgccgggga ggccaucagc uuccccacca      180

cccucggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug ugcgacgcca      240

ccauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac gucgacugcu      300

ggugcaacac gaccagcacc uggguggugu acgggaccug ccaccacaag aagggggagg      360

cgcggcgcuc ccggcgcgcc gucacgcucc ccagccacuc cacccggaag cugcagaccc      420

gcagccagac cuggcuggag ucccgggagu acacgaagca ccucauccgc guggagaacu      480

ggaucuuccg gaaccccggc uucgcccugg ccgcggccgc caucgccugg cugcucggga      540

gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc ccggcguacu      600

ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc ggcggcaccu      660

gggucgacgu ggugcuggag cacgggggcu gcgucaccgu gauggcccag gacaagccca      720

cgguggacau cgagcugguc accaccaccg ugagcaacau ggccgaggug cgcuccuacu      780

gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacg cagggggagg      840

cguaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug guggaccggg      900

gcuggggcaa cgggugcggc cuguucggga agggcagccu cgugaccugc gccaaguucg      960

ccugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag uaccgcauca     1020

ugcuguccgu ccacggcagc cagcacuccg gcaugaucgu gaacgacacc gggcacgaga     1080

ccgacgagaa ccgggccaag gucgagauca ccccgaacag cccccgcgcc gaggcgacgc     1140

ucggcggguu cggcucccug gggcuggacu gcgagccccg gaccggccuc gacuucagcg     1200

accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag ugguuccacg     1260

acaucccccu gccguggcac gccggcgccg acaccgggac gccccacugg aacaacaagg     1320

aggcccuggu ggaguucaag gacgcccacg cgaagcgcca gaccgucgug gugcucggcu     1380

cccaggaggg ggccguccac accgcccugg ccggcgcgcu ggaggccgag auggacgggg     1440

ccaagggccg ccucagcucc ggccaccuga agugccggcu gaagauggac aagcuccgcc     1500

ugaagggggu gagcuacucc cugugcaccg ccgccuucac guucaccaag auccccgcgg     1560

agacccucca cggcaccgug accgucgagg ugcaguacgc cgggacggac ggccccugca     1620

aggugccggc ccagauggcc gucgacaugc agacccugac ccccgugggc cggcugauca     1680

ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc gagcuggacc     1740

cgcccuucgg ggacagcuac aucgucaucg gcguggggga gaagaagauc acccaccacu     1800

ggcaccgcuc cggcagcacc aucgggaagg cguucgaggc cacggugcgg ggcgccaagc     1860

gcauggccgu ccugggcgac accgccuggg acuucgggag cgugggcggg gcgcucaacu     1920

cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug uucggcggga     1980

uguccugguu cagccagauc cugaucggca cccuccugau guggcugggg cucaacacca     2040

agaacggcuc caucagccug augugccugg cccucggggg cgugcugauc uuccugucca     2100

cggcggucag cgccugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2160

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2220

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2280

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2400

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2446


<210>  5728
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 2 GC

<400>  5728
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgccc     1860

gccggauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5729
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 2 GC

<400>  5729
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggauguu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggugauggcc caggacaagc      720

ccaccgugga caucgagcug gucaccacca cggugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acccaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggg agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucacgccgaa cagcccccgc gccgaggcga     1140

cccucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacacggg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacc gugacggucg aggugcagua cgccgggacc gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggga aggcguucga ggccaccgug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccaccgcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5730
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 2 GC

<400>  5730
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caugaccagc accugggugg uguacgggac cugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucacgc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gaccuggcug gagucccggg aguacacgaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac cgugauggcc caggacaagc      720

ccacggugga caucgagcug gucaccacca ccgugagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacag ccggugcccc acgcaggggg      840

aggcguaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacc ugcgccaagu      960

ucgccugcuc caagaagaug acgggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cguccacggc agccagcacu ccggcaugau cgugaacgac accgggcacg     1080

agaccgacga gaaccgggcc aaggucgaga ucaccccgaa cagcccccgc gccgaggcga     1140

cgcucggcgg guucggcucc cuggggcugg acugcgagcc ccggaccggc cucgacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgacaccgg gacgccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgcgaagcg ccagaccguc guggugcucg     1380

gcucccagga gggggccguc cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccgccucagc uccggccacc ugaagugccg gcugaagaug gacaagcucc     1500

gccugaaggg ggugagcuac ucccugugca ccgccgccuu cacguucacc aagauccccg     1560

cggagacccu ccacggcacc gugaccgucg aggugcagua cgccgggacg gacggccccu     1620

gcaaggugcc ggcccagaug gccgucgaca ugcagacccu gacccccgug ggccggcuga     1680

ucaccgccaa ccccgugauc acggagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguca ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc accaucggga aggcguucga ggccacggug cggggcgcca     1860

agcgcauggc cguccugggc gacaccgccu gggacuucgg gagcgugggc ggggcgcuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

ccaagaacgg cuccaucagc cugaugugcc uggcccucgg gggcgugcug aucuuccugu     2100

ccacggcggu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5731
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 2 GC

<400>  5731
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacca cggccauggc cgcggaggug acccgccggg      120

gcuccgccua cuacauguac cuggaccgca acgacgccgg ggaggccauc agcuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

ccacgauguc cuacgagugc ccgaugcugg acgagggcgu ggagcccgac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac gugccaccac aagaaggggg      360

aggcgcggcg cucccggcgc gccgucaccc uccccagcca cuccacccgg aagcugcaga      420

cccgcagcca gacguggcug gagucccggg aguacaccaa gcaccucauc cgcguggaga      480

acuggaucuu ccggaacccc ggcuucgccc uggccgcggc cgccaucgcc uggcugcucg      540

ggagcuccac cagccagaag gugaucuacc uggucaugau ccugcucauc gccccggcgu      600

acuccauccg cugcaucggc gugagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggucga cguggugcug gagcacgggg gcugcgucac ggccauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca cggucagcaa cauggccgag gugcgcuccu      780

acugcuacga ggccagcauc uccgacaugg cgagcgacag ccggugcccc acccaggggg      840

aggccuaccu cgacaagcag uccgacaccc aguacgugug caagcgcacc cuggucgacc      900

ggggcugggg caacgggugc ggccuguucg ggaagggcag ccucgugacg ugcgccaagu      960

ucgccugcuc caagaagaug accggcaaga gcauccagcc cgagaaccug gaguaccgca     1020

ucaugcuguc cgugcacggg agccagcacu ccggcaugcu cgucaacgac accgggcacg     1080

agaccgacga gaaccgggcc aagguggaga ucacgccgaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu ggcccacaag gagugguucc     1260

acgacauccc ccugccgugg cacgccggcg ccgcgacggg gacgccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggcggug cacaccgccc uggccggcgc ccucgaggcc gagauggacg     1440

gggcgaaggg ccgccugagc uccggccacc ugaagugccg gcucaagaug gacaagcugc     1500

gccugaaggg ggugagcuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagaccgu cgacggcacc gugacggugg aggggcagua cggcggcacc gacgggcccu     1620

gcaagguccc ggcccagaug gcgguggaca ugcagacccu gacccccgug ggccggcuga     1680

ucacggccaa ccccgucauc accgagagca ccgagaacuc caagaugaug cucgagcugg     1740

acccgcccuu cggggacagc uacaucguga ucggcguggg ggagaagaag aucacccacc     1800

acuggcaccg cuccggcagc acgaucggca aggccuucga ggccaccguc cgcggggcca     1860

agcggauggc ggugcugggc gacaccgccu gggacuucgg gagcgugggc ggggcccuca     1920

acucccuggg caagggcauc caccagauca ucggggccgc guucaagagc cuguucggcg     1980

ggauguccug guucagccag auccugaucg gcacccuccu gauguggcug gggcucaaca     2040

cgaagaacgg cuccaucagc cugaugugcc uggcccucgg cgggguccug aucuuccugu     2100

ccaccgccgu gagcggcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5732
<211>  2440
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 2 GC

<400>  5732
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacgccag caucgggauc gugucccugc uccugaccac cgucauggcg gccgagauca      120

cgcgccgggg cagcgccuac uacauguacc uggaccgcuc cgacgccggg aaggccauca      180

gcuucgugac cacccucggc gugaacaagu gccacgucca gaucauggac cuggggcaca      240

ugugcgacgc gaccaugucc uacgagugcc ccaugcugga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac acgaccagca ccugggucgu guacgggacc ugccaccaca      360

agaaggggga ggcccggcgc ucccggcgcg ccgugacgcu ccccagccac uccacccgga      420

agcugcagac ccgcagccag accuggcugg agucccggga guacacgaag caccucauca      480

aggucgagaa cuggaucuuc cgcaaccccg gcuucgcccu gguggcggug gccaucgccu      540

ggcugcucgg gagcuccacc agccagaagg ucaucuaccu ggugaugauc cugcucaucg      600

cccccgccua cuccauccgg ugcaucggcg ugagcaaccg cgacuucguc gaggggaugu      660

ccggcggcac cuggguggac gugguccugg agcacggggg cugcgugacc gugauggcgc      720

aggacaagcc gacggucgac aucgagcugg ugaccaccac cgugagcaac auggccgagg      780

uccgguccua cugcuacgag gccagcauca gcgacauggc cuccgacagc cgcugcccca      840

cgcaggggga ggccuaccuc gacaagcagu ccgacaccca guacgugugc aagcggaccc      900

uggucgaccg cggcuggggc aacgggugcg gccuguucgg gaagggcagc cucgugaccu      960

gcgcgaaguu cacgugcucc aagaagauga ccggcaagag cauccagccc gagaaccugg     1020

aguaccggau caugcugucc gugcacggca gccagcacuc cggcaugauc gucaacgacg     1080

agaaccgcgc caagguggag gugaccccca acagcccgcg ggccgaggcc acccucgggg     1140

gcuucggguc ccugggccug gacugcgagc cccgcacggg gcucgacuuc agcgaccugu     1200

acuaccugac caugaacaac aagcacuggc ucguccacaa ggagugguuc cacgacaucc     1260

cccugcccug gcacuccggc gccgacaccg agaccccgca cuggaacaac aaggaggcgc     1320

ugguggaguu caaggacgcc cacgccaagc ggcagacggu ggucgugcuc ggcagccagg     1380

agggggccgu gcacaccgcc cuggcgggcg cccuggaggc cgagauggac ggggccaagg     1440

gccgccucuc cagcgggcac cugaagugcc gccugaagau ggacaagcuc cggcugaagg     1500

gcgucuccua cagccugugc accgcggccu ucaccuucac gaaggugccc gccgagaccc     1560

uccacggcac cgugaccguc gaggugcagu acgccgggcg cgacggcccc ugcaaggugc     1620

ccgcccagau ggcggucgac augcagaccc ugacgccggu ggggcggcug aucaccgcca     1680

accccgugau caccgagucc accgagaaca gcaagaugau gcucgagcug gacccgcccu     1740

ucggcgacag cuacaucguc aucggcgugg gggacaagaa gaucacgcac cacuggcacc     1800

gcuccggcag caucaucggg aaggccuucg aggccaccgu gcggggcgcc aagcgcaugg     1860

cgguccuggg ggacaccgcc ugggacuucg gcuccguggg cgggguguuc aacagccucg     1920

gcaaggggau ccaccagauc uucggcgccg ccuucaaguc ccuguucggg ggcaugagcu     1980

gguucuccca gauccugauc ggcacccugc ucgucuggcu ggggcugaac acgaagaacg     2040

gcagcaucuc ccugaccugc cuggcccucg ggggcgugau gaucuuccuc agcaccgcgg     2100

uguccgccug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2160

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2220

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2280

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2400

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2440


<210>  5733
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 2 GC

<400>  5733
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag caucgggauc aucggccugc uccugaccac ggccauggcg gccgagauca      120

cccgccgggg guccgccuac uacauguacc uggaccgcag cgacgccggc aaggccaucu      180

ccuucgcgac caccaugggg gugaacaagu gccacgucca gaucauggac cucggccaca      240

ugugcgacgc cacgaugagc uacgagugcc ccaugcugga cgagggcgug gagccggacg      300

acguggacug cuggugcaac accaccucca ccugggucgu guacgggacg ugccaccaca      360

agaagggcga ggcccggccc agccgccggg ccgugacccu gcccucccac agcacccgca      420

agcuccagac ccggucccag acguggcugg agagccgcga guacaccaag caccugauca      480

aggucgagaa cuggauccuc cggaaccccg gguucaccgu ggcggcccug gccauggccu      540

ggcugcucgg caacuccacc agccagcgcg ugaucuaccu ggucaugauc cugcucaucg      600

ccccggcgua cuccauccgg ugcaucgggg ugagcaaccg cgacuucgug gagggcaugu      660

ccggcgggac gugggucgac guggugcugg agcacggcgg gugcgucacc gugauggccc      720

aggacaagcc caccguggac aucgagcugg ucaccacgac cgugagcaac auggccgagg      780

ugcgguccua cugcuacgag gccagcaucu ccgacauggc cagcgacagc cgcugcccca      840

cccagggcga ggcguaccuc gacaagcagu ccgacaccca guacgucugc aagcggacgc      900

ugguggaccg cggcuggggg aacggcugcg ggcuguucgg caaggggagc cucgugaccu      960

gcgccaaguu cacgugcucc aagaagauga ccggcaagag cauccagccc gagaaccugg     1020

aguaccggau caugcugucc guccacggca gccagcacuc cgggaugauc gugaacgaca     1080

ucggccacga gaccgacgag aaccgcgcca aggucgaggu gaccccgaac agcccccggg     1140

ccgaggccac ccucgggggc uucggguccc ugggccugga cugcgagccc cgcaccggcc     1200

ucgacuucag cgaccuguac uaccugacga ugaacaacaa gcacuggcuc gugcacaagg     1260

agugguucca cgacaucccc cugccguggc acgcgggggc cgacaccggc acgccccacu     1320

ggaacaacaa ggaggcccug gucgaguuca aggacgccca cgccaagcgg cagaccgugg     1380

ugguccucgg gucccaggag ggcgcggugc acaccgcccu ggccggggcc cuggaggcgg     1440

agauggacgg cgccaagggc cgccucuuca gcgggcaccu gaagugccgc aucaagaugg     1500

acaagcugcg gcucaagggc guguccuaca gccugugcac cgccgccuuc accuucucca     1560

agguccccgc cgagacccug cacgggaccg ugacggugga gguccaguac gcgggccgcg     1620

acggccccug caaggugccg gcccagaugg ccguggacau gcagacccuc acccccgucg     1680

ggcggcugau caccgccaac cccaucauca cggagagcac cgagaacucc cgcaugaugc     1740

uggagcucga cccgcccuuc ggcgacagcu acaucgugau cggggugggc gacaagaaga     1800

ucacccacca cuggcaccgg uccgggagca ccaucggcaa ggccuucgag gcgacggucc     1860

gcggcgccaa gcggauggcc gugcuggggg acaccgccug ggacuucggc agcgucgggg     1920

gcguguucaa cucccugggg aagggcgugc accagguguu cggcgccgcg uucaagagcc     1980

ucuucggggg cauguccugg uucagccaga uccugaucgg gacccugcug gucuggcucg     2040

gccugaacac caagaacggg uccaucagcc uggccugccu cgcccugggc ggcgccauga     2100

ucuuccuguc cacggcggug agcgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  5734
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 2 GC

<400>  5734
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      120

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      180

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      240

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      360

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      420

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgugc      540

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      660

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      720

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc      780

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg      900

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc      960

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1020

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1080

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1140

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1200

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1320

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1380

ugggggacac cgcgugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5735
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 2 GC

<400>  5735
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      120

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg guggacgucg      180

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      240

agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      360

agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc      540

acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      660

gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc      780

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc caggaggggg      900

ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc      960

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1020

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acgcuccacg     1080

gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1140

agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg     1200

ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg caccggagcg     1320

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1380

ugggggacac ggcgugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5736
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 2 GC

<400>  5736
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggugau cuaccucguc augauccugc ugaucgcccc ggccuacagc auccgcugca      120

ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg gucgacgugg      180

ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc guggacaucg      240

agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga      480

agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgugc      540

acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg gccaaggugg      600

aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc agccucgggc      660

uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug acgaugaaca      720

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg uggcacgcgg      780

gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag uucaaggacg      840

cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg guccacacgg      900

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug uucuccgggc      960

accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc uacucccucu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc acggucaccg     1080

uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag auggcggugg     1140

acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc aucaccgaga     1200

gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac agcuacaucg     1260

ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1320

ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc ggggacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  5737
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 2 GC

<400>  5737
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      120

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacgucg      180

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      240

agcucgugac cacgaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      360

agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      420

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc      540

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacg gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      660

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      720

ucacgaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc      780

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcgcacgcc aagcgccaga ccguggucgu gcugggcucc caggaggggg      900

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc      960

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1020

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1080

gcacggugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugacgc ccgucgggcg ccugaucacc gcgaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggc gucggggaga agaagaucac gcaccacugg caccggagcg     1320

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1380

ugggggacac cgcgugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5738
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 2 GC

<400>  5738
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg cuacgagacc gacgagaacc      600

gggccaaggu ggaggucacg cccaacuccc cgcgcgccga ggccacccuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc gggggccgac acgggcaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcuggggagc caggagggcg      900

cgguccacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg aagggcaagc      960

uguucagcgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcgugu     1020

ccuacagccu cugcacggcc gccuucaccu ucaccaaggu ccccgccgag acccugcacg     1080

ggacggugac cguggagguc caguacgccg gcaccgacgg ccccugcaag auccccgugc     1140

agauggcggu ggacaugcag acccugacgc cggucgggcg gcucaucacc gccaaccccg     1200

ugaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggg gucggcgaca agaagaucac gcaccacugg caccgcagcg     1320

gguccaccau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggugc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5739
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 2 GC

<400>  5739
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaccguga acgacaucgg cuacgagacc gacgagaacc      600

gggccaaggu ggaggucacg cccaacuccc cgcgcgccga ggccacccuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc gggggccgac acgggcaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcuggggagc caggagggcg      900

cgguccacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg aagggcaagc      960

uguucagcgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcgugu     1020

ccuacagccu cugcacggcc gccuucaccu ucaccaaggu ccccgccgag acccugcacg     1080

ggacggugac cguggagguc caguacgccg gcaccgacgg ccccugcaag auccccgugc     1140

agauggcggu ggacaugcag acccugacgc cggucgggcg gcucaucacc gccaaccccg     1200

ugaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggg gucggcgaca agaagaucac gcaccacugg caccgcagcg     1320

gguccaccau cggcaaggcc uucgaggcca ccgugcgggg cgccaagcgc auggcggugc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5740
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 2 GC

<400>  5740
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcggccu cuucgggaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacgagaa ccgggccaag guggagguca      600

cccccaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccug ggccucgacu      660

gcgagccccg gacggggcug gacuucuccg accuguacua ccucaccaug aacaacaagc      720

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gcgggcgccg      780

acaccgggac cccgcacugg aacaacaagg aggcccucgu ggaguucaag gacgcccacg      840

ccaagcgcca gacggucgug gugcugggca gccaggaggg ggcgguccac accgcccugg      900

ccggcgcccu cgaggccgag auggacggcg cgaaggggcg gcuguucagc ggccaccuga      960

agugccgccu caagauggac aagcugcggc ugaagggggu guccuacagc cucugcaccg     1020

ccgccuucac cuucacgaag guccccgccg agacccugca cggcaccgug accguggagg     1080

uccaguacgc cgggacggac ggccccugca aggugcccgc gcagauggcc guggacaugc     1140

agacccugac cccggucggc cgccucauca ccgccaaccc cgugaucacg gaguccaccg     1200

agaacagcaa gaugaugcug gagcuggacc cgcccuucgg ggacuccuac aucgugaucg     1260

gcgucgggga caagaagauc acccaccacu ggcaccggag cggcuccacc aucgggaagg     1320

ccuucgaggc cacggugcgc ggcgcgaagc ggauggccgu gcucggcgac accgccuggg     1380

acuucgggag cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  5741
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 2 GC

<400>  5741
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucggcu acgagaccga cgagaaccgg gccaaggugg      600

aggugacccc caacuccccg cgcgccgagg ccacgcucgg gggcuucggc agccuggggc      660

ucgacugcga gccccggacc ggccuggacu ucuccgaccu guacuaccuc accaugaaca      720

acaagcacug gcugguccac aaggaguggu uccacgacau cccccugccc uggcacgcgg      780

gggccgacac cggcacgccg cacuggaaca acaaggaggc ccucguggag uucaaggacg      840

cccacgccaa gcgccagacc guggucgugc uggggagcca ggagggcgcg gugcacaccg      900

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggcaagcug uucagcgggc      960

accugaagug ccggcucaag auggacaagc ugcgccugaa gggcgucucc uacagccucu     1020

gcaccgccgc cuucacguuc accaaggugc ccgccgagac ccugcacggg accgucacgg     1080

uggaggugca guacgccggc accgacggcc ccugcaagau ccccguccag auggcggugg     1140

acaugcagac ccugaccccg guggggcggc ucaucacggc caaccccguc aucaccgagu     1200

ccaccgagaa cagcaagaug augcuggagc uggacccgcc cuucggcgac uccuacaucg     1260

ugaucggggu gggcgacaag aagaucaccc accacuggca ccgcagcggg uccacgaucg     1320

gcaaggccuu cgaggccacc guccggggcg ccaagcgcau ggcggugcuc ggggacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  5742
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 2 GC

<400>  5742
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc cugcgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcucgagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcuggucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccucgu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcuggacugc gagccccgga ccggccugga cuucuccgac cucuacuacc      720

ugaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccucgcc ggcgcccugg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccucaag ugccgccuga agauggacaa gcugcggcuc aagggcguca     1020

gcuacucccu gugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccucaccc cgguggggcg ccugaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcucgacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ugggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5743
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 2 GC

<400>  5743
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagca       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5744
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 2 GC

<400>  5744
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg uaccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5745
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 2 GC

<400>  5745
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggcaagc      960

ugagcuccgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg gcucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcuccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgggg cgccaagcgc auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5746
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 2 GC

<400>  5746
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacacccag acccugacgc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac gcaccacugg caccgguccg     1320

ggagcaccau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5747
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 2 GC

<400>  5747
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgagauccg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgugc      540

acggcuccca gcacagcggg augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcg aagcgccaga ccgugguggu ccuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguga     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgucac gguggagguc caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc cggucgggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5748
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 2 GC

<400>  5748
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgcccggcgc auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5749
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 2 GC

<400>  5749
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggucagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc caaguucgcg ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu cgagaucacc cccaacuccc cgcgcgccga ggccacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc gggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcuggggagc caggagggcg      900

cgguccacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguga     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgucac gguggaggug caguacgccg gcaccgacgg ccccugcaag guccccgcgc     1140

agauggccgu ggacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgugaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcca ccguccgcgg cgcgaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5750
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 2 GC

<400>  5750
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaucgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcaccau cggcaaggcc uucgaggcga cggugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5751
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 2 GC

<400>  5751
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg aucggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5752
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 2 GC

<400>  5752
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccu ggccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcucgagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcuggucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccucgu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cccgcgccga ggcgacgcuc gggggcuucg      660

gcucccuggg gcuggacugc gagccgcgga ccggccugga cuucuccgac cucuacuacc      720

ugaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggggccgac accggcacgc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccucgcc ggcgcccugg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccucaag ugccgccuga agauggacaa gcugcggcuc aagggcguca     1020

gcuacucccu gugcaccgcc gccuucacgu ucaccaagau cccggccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccucaccc ccguggggcg ccugaucacg gccaacccgg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcucgacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ugggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5753
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 2 GC

<400>  5753
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggacc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcagcgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcgucu     1020

ccuacagccu cugcacggcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

ggacggugac cgucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugacgc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgucaucggg gugggcgaga agaagaucac gcaccacugg caccggagcg     1320

gguccaccau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5754
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 2 GC

<400>  5754
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcuggccc     1140

agauggccgu cgacaugcag acccucaccc ccguggggcg ccugaucacg gccaacccgg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcucgacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ugggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5755
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 2 GC

<400>  5755
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga ggcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5756
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 2 GC

<400>  5756
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcggcccuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga cgggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcgcucg aggccgagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucaccu ucacgaagau ccccgcggag acccugcacg     1080

ggaccgugac cgucgaggug caguacgccg gcacggacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucacgga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcaccau cggcaaggcg uucgaggcca cggugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgcgugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5757
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 2 GC

<400>  5757
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucag caccaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggcca      300

ccaucuccga caucgccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccgccguggu ccuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcgcucg aggccgagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguga     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgcggag acccugcacg     1080

ggaccgucac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc cggucgggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcg uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1380

ucggggacac cgcgugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5758
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 2 GC

<400>  5758
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucagcaagau ccccgccgag acccugcacg     1080

ggaccgugac cgaggagguc caguacgcgg gcacggacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc cggucgggcg ccucaucacc gccaaccccg     1200

ugaucacgga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccggagcg     1320

gguccaccau cggcaaggcc uucgaggcga cggugcgcgg cgccaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5759
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 2 GC

<400>  5759
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgagcug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccucaccc cggucgggcg ccugaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcucgacccg cccuucggcg     1260

acagcuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgugc     1380

ugggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5760
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 2 GC

<400>  5760
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcaucag caaccgggac uucguggagg ggauguccgg cgggaccugg guggacgucg      180

ugcuggagca cggcgggugc gugaccguca uggcgcagga caagcccacg guggacaucg      240

agcucgugac caccaccguc agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgugc      540

acggcuccca gcacagcggg augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcauccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcg aagcgccaga ccgugguggu ccuggggucc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcagcgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcgugu     1020

ccuacagccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgucac gguggagguc caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc cggucgggcg ccucaucacg gccaaccccg     1200

ugaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggg gucggcgaga agaagaucac ccaccacugg caccggagcg     1320

gguccacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5761
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 2 GC

<400>  5761
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacgggguc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguga     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgucac gguggaggug caguacgccg gcaccgacgg ccccugcaag guccccgcgc     1140

agauggccgu ggacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgugaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcca ccguccgcgg cgcgaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5762
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 2 GC

<400>  5762
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcgccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucacgga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcaccau cggcaaggcg uucgaggcca cggugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgcgugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5763
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 2 GC

<400>  5763
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacac cggggccgac acgggcaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcc aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

cggugcacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcacggcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

ggacggugac cgucgaggug caguacgccg gcaccgacgg ccccugcaag gugcccgcgc     1140

agauggccgu cgacaugcag acccugacgc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucaccga gggcaccgag aacagcaaga ugaugcugga gcuggacccg cccuucgggg     1260

acuccuacau cgucaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1320

gcuccaccau cggcaaggcc uucgaggcca ccgugcgcgg ggcgaagcgg auggccgucc     1380

ucggcgacac cgccugggac uucgggagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5764
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 2 GC

<400>  5764
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccgcga uggcccagga caagcccacg guggacaucg      240

agcucgugac caccaccguc agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcg uaccuggaca      360

agcagagcga cacccaguac gugugcaagc gcacccuggu cgaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgugc      540

acggcuccca gcacagcggg augcugguca acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgcgga ggccacgcuc gggggcuucg      660

gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac cucuacuacc      720

ugaccaugaa caacaagcac uggcuggccc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggggccgcg accggcacgc cgcacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcuggggagc caggagggcg      900

ccguccacac cgcgcucgcc ggcgcccugg aggccgagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccucaag ugccgccuga agauggacaa gcugcggcuc aagggcguga     1020

gcuacucccu gugcaccgcg gccuucacgu ucaccaagau ccccgccgag accguggacg     1080

ggaccgucac gguggagggc caguacggcg ggaccgacgg ccccugcaag guccccgccc     1140

agauggccgu ggacaugcag acccugaccc cgguggggcg ccucaucacg gcgaaccccg     1200

ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgugaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

gcagcacgau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggcggugc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5765
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 2 GC

<400>  5765
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccgcc guggacaucg      240

agcucgucac gaccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccaccca gggcgaggcg uaccuggaca      360

agcagagcga cacgcaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacacggg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgcgga ggccacccuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga cgggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcgcucg aggccgagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucaccu ucacgaagau ccccgcggag acccugcacg     1080

ggaccgugac cgucgaggug caguacgccg gcacggacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucacgga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcaccau cggcaaggcg uucgaggcca cggugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgcgugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5766
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 2 GC

<400>  5766
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugaa caaccgggac uucguggagg ggaugagcgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug uccaacaugg ccgaggugcg cagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccggu gccccacgca gggcgaggcc uaccuggaca      360

agcaguccga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggagccugg ugaccugcgc gaaguucgcc ugcuccaaga      480

agaugacggg caagagcauc cagcccgaga accuggagua ccgcaucaug cucuccgucc      540

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacagcc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggucc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcagcgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcgucu     1020

ccuacagccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ugaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccggagcg     1320

gguccacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5767
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 2 GC

<400>  5767
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcgccgcc gccuucaccu ucacgaagau ccccgcggag acccugcacg     1080

ggaccgugac cgucgaggug caguacgccg gcacggacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucacgga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgguccg     1320

ggagcaccau cggcaaggcg uucgaggcca cggugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgcgugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5768
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 2 GC

<400>  5768
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaucgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacccuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga cgggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga cgguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucaccu ucacgaagau ccccgccgag acccugcacg     1080

ggaccgugac cgucgaggug caguacgcgg gcacggacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucacgga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaca agaagaucac ccaccacugg caccgguccg     1320

ggagcaccau cggcaaggcc uucgaggcga cggugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5769
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 2 GC

<400>  5769
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucgcc ugcagcaaga      480

agaugacggg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggagaucacc cccaacuccc cgcgcgccga ggcgacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcgccaga ccguggucgu gcuggggagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccggc      960

ugagcuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcguca     1020

gcuacucccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacg gccaaccccg     1200

ugaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg cccuucggcg     1260

acagcuacau cgucaucggg gugggcgaca agaagaucac ccaccacugg uaccgguccg     1320

ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5770
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 2 GC

<400>  5770
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gggccaaggu ggaggucacg cccaacuccc cgcgcgccga ggccacccuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc gggggccgac acgggcaccc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcuggggagc caggagggcg      900

cgguccacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg aagggccggc      960

uguucagcgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcgugu     1020

ccuacagccu cugcacggcc gccuucaccu ucaccaaggu ccccgccgag acccugcacg     1080

ggacggugac cguggagguc caguacgccg gcaccgacgg ccccugcaag gugcccgcgc     1140

agauggccgu ggacaugcag acccugacgc cggucgggcg ccucaucacc gccaaccccg     1200

ugaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggg gucggcgaca agaagaucac gcaccacugg caccggagcg     1320

gguccaccau cggcaaggcc uucgaggcca ccgugcgcgg cgcgaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5771
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 2 GC

<400>  5771
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaucgg ccacgagacc gacgagaacc      600

gggccaaggu ggaggucacc cccaacuccc cgcgcgccga ggccacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc gggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcuggggagc caggagggcg      900

cgguccacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg aagggccggc      960

uguucagcgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcgugu     1020

ccuacagccu cugcaccgcc gccuucacgu ucaccaaggu ccccgccgag acccugcacg     1080

ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag gugcccgcgc     1140

agauggccgu ggacaugcag acccugaccc cggucgggcg ccucaucacg gccaaccccg     1200

ugaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgugaucggg gucggcgaca agaagaucac ccaccacugg caccggagcg     1320

gguccacgau cggcaaggcc uucgaggcca ccgugcgcgg cgcgaagcgg auggccgugc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5772
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 2 GC

<400>  5772
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaucgg ccacgagacc gacgagaacc      600

gggccaaggu ggaggucacc cccaacuccc cgcgcgccga ggccacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc gggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcuggggagc caggagggcg      900

cgguccacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg aagggccggc      960

uguucagcgg gcaccugaag ugccgccuca agauggacaa gcugcggcug aagggcgugu     1020

ccuacagccu cugcaccgcc gucugcacgg ccgccaaggu gcccgccgag acccugcacg     1080

ggaccgugac cgucgaggug caguacgcgg gcacggacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg ccucaucacc gccaaccccg     1200

ugaucacgga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgucaucggg gugggcgaca agaagaucac ccaccacugg caccggagcg     1320

gguccaccau cggcaaggcc uucgaggcga cggugcgcgg cgccaagcgg auggccgucc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5773
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 2 GC

<400>  5773
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccgcugca      120

ucggcgugag caaccgggac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg cuccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccggu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      420

gcugcggccu cuucgggaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccgcaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacgagaa ccgggccaag guggagguca      600

cccccaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccug ggccucgacu      660

gcgagccccg gacggggcug gacuucuccg accuguacua ccucaccaug aacaacaagc      720

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac agcggcgcgg      780

acaccgagac cccgcacugg aacaacaagg aggcccucgu ggaguucaag gacgcccacg      840

ccaagcgcca gacggucgug gugcugggga gccaggaggg cgccguccac accgcgcugg      900

ccggggcccu cgaggccgag auggacggcg ccaagggccg gcuguccagc gggcaccuga      960

agugccgccu caagauggac aagcugcggc ugaagggcgu guccuacagc cucugcaccg     1020

cggccuucac cuucacgaag guccccgccg agacccugca cgggaccgug accguggagg     1080

uccaguacgc cggccgcgac gggcccugca aggugcccgc ccagauggcg guggacaugc     1140

agacgcugac cccggucggc cggcucauca ccgccaaccc cgugaucacc gaguccacgg     1200

agaacagcaa gaugaugcug gagcuggacc cgcccuucgg cgacuccuac aucgugaucg     1260

gggucggcga caagaagauc acccaccacu ggcaccgcag cggguccauc aucggcaagg     1320

ccuucgaggc caccgugcgc ggggccaagc ggauggcggu gcucggcgac accgccuggg     1380

acuucggcag cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  5774
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 2 GC

<400>  5774
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augaucgugc ucaucgugcc cgccuacucc aucagcugca      120

ucggcgucuc caaccgcgac cugguggagg ggaugagcgg cgggaccugg guggacgucg      180

ugcuggagca cggcgggugc gugaccgaga uggcccagga caagcccacg gucgacaucg      240

agcucgugac caugaccgug uccaacaugg cggagguccg gagcuacugc uacgaggccu      300

cccugagcga cauggccucc gccagccgcu gccccaccca gggcgagccc ucccuggaca      360

agcagagcga cacgcagucc gugugcaagc ggacccucgg cgaccgcggg uggggcaacg      420

ggugcggcau cuucgggaag ggcagccugg ugaccugcuc caaguucacg ugcugcaaga      480

agaugcccgg caagagcauc cagccggaga accuggagua ccggaucaug cuccccgucc      540

acggguccca gcacagcggc augaucguga acgacaucgg gcacgagacc gacgagaacc      600

gcgccaaggu ggaggucacc cccaacagcc cccgggcgga ggccacccug ggcggcuucg      660

ggucccuggg ccucgacugc gagccgcgca ccgggcugga cuucagcgac cuguacuacc      720

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accgggacgc cccacuggaa caacaaggag gcgcucgugg      840

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcugggcucc caggagggcg      900

ccguccacac cgcccuggcg ggggcccucg aggccgagau ggacggcgcc aaggggcgcc      960

uguucagcgg ccaccugaag ugccggcuca agauggacaa gcugcgccug aagggggugu     1020

ccuacagccu cugcaccgcc gcguucaccu ucaccaaggu cccggccgag acgcugcacg     1080

gcaccgugac cguggagguc caguccgccg gcaccgacgg gcccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acgcugaccc ccgucggccg gcucaucacc gcgaacccgg     1200

ugaucaccga gagcacggag aacuccaaga ugaugcugga gcuggacccg cccuucgggg     1260

acagcuacau cgugaucggc gucggggaca agaagaucac ccaccacugg caccgcuccg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugcgcgg ggccaagcgg auggccgugc     1380

ucggcgacac ggcgugggac uucggguccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5775
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 2 GC

<400>  5775
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

agaaggugau cuaccugguc augauccucc ugaucgcccc cgccuacagc auccggugca      120

ucggcguguc caaccgcgac uucguggagg ggaugagcgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug uccaacaugg ccgaggugcg gagcuacugc uacgaggccu      300

ccaucagcga cauggccucc gacagccgcu gccccacgca gggcgaggcc uaccuggaca      360

agcaguccga cacccaguac gucugcaagc ggacccuggu ggaccgcggc ugggggaacg      420

gcugcgggcu guucggcaag gggagccugg ugaccugcgc gaaguucgcc ugcuccaaga      480

agaugacggg caagagcauc cagcccgaga accuggagua ccggaucaug cucuccgucc      540

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu ggagaucacc cccaacagcc cgcgggccga ggcgacgcuc gggggcuucg      660

gcucccuggg gcucgacugc gagccccgca ccggccugga cuucagcgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcg aagcggcaga ccguggucgu gcuggggucc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccucg aggcggagau ggacggggcc aagggccgcc      960

ugagcagcgg gcaccugaag ugccggcuca agauggacaa gcugcgccug aagggcgucu     1020

ccuacagccu cugcaccgcc gccuucacgu ucaccaagau ccccgccgag acccugcacg     1080

ggaccgugac ggucgaggug caguacgcgg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu cgacaugcag acccugaccc cgguggggcg gcucaucacg gccaaccccg     1200

ugaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg cccuucggcg     1260

acuccuacau cgucaucggg gugggcgaga agaagaucac ccaccacugg caccgcagcg     1320

gguccacgau cggcaaggcc uucgaggcga ccgugcgggg cgccaagcgc auggccgucc     1380

ucggggacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5776
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 2 GC

<400>  5776
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agcgcgugau cuaccugguc augauccucc ugaucgcccc cgccuacucc auccggugca      120

ucggcgugag caaccgcgac uucguggagg ggauguccgg cgggaccugg gucgacgugg      180

ugcuggagca cggcgggugc gucaccguga uggcgcagga caagcccacg guggacaucg      240

agcucgucac caccaccgug agcaacaugg ccgaggugcg guccuacugc uacgaggcca      300

gcaucuccga cauggccagc gacucccgcu gccccacgca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gucugcaagc ggacccuggu ggaccgcggc ugggggaacg      420

gcugcgggcu guucggcaag gggucccugg ugaccugcgc gaaguucacg ugcagcaaga      480

agaugaccgg caaguccauc cagcccgaga accuggagua ccggaucaug cucagcgucc      540

acggcuccca gcacagcggg augaucguga acgacaucgg ccacgagacc gacgagaacc      600

gcgccaaggu ggaggucacc cccaacuccc cgcgggccga ggccacgcuc gggggcuucg      660

gcagccuggg gcucgacugc gagccccgca ccggccugga cuucuccgac cuguacuacc      720

ucaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc gggggccgac accggcacgc cgcacuggaa caacaaggag gcccucgugg      840

aguucaagga cgcccacgcc aagcggcaga ccgucguggu gcuggggagc caggagggcg      900

cgguccacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg aagggccgcc      960

uguucagcgg gcaccugaag ugccggauca agauggacaa gcuccgccug aagggcgugu     1020

ccuacagccu gugcaccgcc gccuucacgu ucuccaaggu ccccgccgag acccuccacg     1080

ggaccgugac cguggagguc caguacgccg gccgggacgg ccccugcaag gugcccgcgc     1140

agauggccgu ggacaugcag acgcugaccc cggucgggcg ccugaucacc gccaacccca     1200

ucaucaccga gagcacggag aacucccgga ugaugcucga gcuggacccg cccuucggcg     1260

acagcuacau cgugaucggg gugggcgaca agaagaucac ccaccacugg caccgcuccg     1320

ggagcaccau cggcaaggcc uucgaggcca ccguccgggg cgcgaagcgc auggccgugc     1380

ugggggacac ggccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5777
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  5777
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5778
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 opt

<400>  5778
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaccug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacgccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5779
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opt

<400>  5779
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucggcu acgagaccga cgaggaccgc gccaaggucg     1140

aggucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc     1200

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca     1260

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg     1440

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uucuccggcc     1500

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1560

gcaccgccgc cuucaccuuc accaaggucc ccgccgagac ccuccacggc accgucaccg     1620

ucgaggucca guacgccggc accgacggcc ccugcaagau ccccguccag auggccgucg     1680

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1740

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1800

ucaucggcgu cggcgacaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1860

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1920

ccugggacuu cggcuccguc ggcggcgucu ucaacucccu cggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc cagauccuca     2040

ucggcacccu ccucgucugg cucggccuca acaccaagaa cggcuccauc ucccucaccu     2100

gccucgcccu cggcggcguc augaucuucc ucuccaccgc cgucuccgcc gacgucggcu     2160

gcuccgucga cuucuccaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5780
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 2 opt

<400>  5780
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5781
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  5781
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5782
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 2 opt

<400>  5782
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac accugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaacg     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5783
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 2 opt

<400>  5783
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cggcuacgag accgacgagg     1080

accgcgccaa ggucgagguc acccccaacu ccccccgcgc cgaggccacc cucggcggcu     1140

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu     1200

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1260

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1320

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1380

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1440

gccucuucuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1500

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gguccccgcc gagacccucc     1560

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagauccccg     1620

uccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1680

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1740

gcgacuccua caucgucauc ggcgucggcg acaagaagau cacccaccac uggcaccgcu     1800

ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag cgcauggccg     1860

uccucggcga caccgccugg gacuucggcu ccgucggcgg cgucuucaac ucccucggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc auguccuggu     1980

ucucccagau ccucaucggc acccuccucg ucuggcucgg ccucaacacc aagaacggcu     2040

ccaucucccu caccugccuc gcccucggcg gcgucaugau cuuccucucc accgccgucu     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5784
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 2 opt

<400>  5784
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5785
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 opt

<400>  5785
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5786
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 opt

<400>  5786
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5787
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opt

<400>  5787
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucggcu acgagaccga cgaggaccgc gccaaggucg      600

aggucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc      660

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca      720

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg      780

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg      840

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg      900

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uucuccggcc      960

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1020

gcaccgccgc cuucaccuuc accaaggucc ccgccgagac ccuccacggc accgucaccg     1080

ucgaggucca guacgccggc accgacggcc ccugcaagau ccccguccag auggccgucg     1140

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1200

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1260

ucaucggcgu cggcgacaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1320

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1380

ccugggacuu cggcuccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  5788
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 2 opt

<400>  5788
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  5789
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       2 opt

<400>  5789
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuccg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5790
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 2
       opt

<400>  5790
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac accugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuccg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaacg     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5791
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 2 
       opt

<400>  5791
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuccg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cggcuacgag accgacgagg     1080

accgcgccaa ggucgagguc acccccaacu ccccccgcgc cgaggccacc cucggcggcu     1140

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu     1200

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1260

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1320

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1380

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1440

gccucuucuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1500

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gguccccgcc gagacccucc     1560

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagauccccg     1620

uccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1680

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1740

gcgacuccua caucgucauc ggcgucggcg acaagaagau cacccaccac uggcaccgcu     1800

ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag cgcauggccg     1860

uccucggcga caccgccugg gacuucggcu ccgucggcgg cgucuucaac ucccucggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc auguccuggu     1980

ucucccagau ccucaucggc acccuccucg ucuggcucgg ccucaacacc aagaacggcu     2040

ccaucucccu caccugccuc gcccucggcg gcgucaugau cuuccucucc accgccgucu     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5792
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 2 
       opt

<400>  5792
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuccg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5793
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       2 opt

<400>  5793
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cguccaggac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5794
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 2
       opt

<400>  5794
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac accugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cguccaggac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaacg     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5795
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 2 
       opt

<400>  5795
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cguccaggac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5796
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  5796
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc aagggcgccc     1860

agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc ggcgucuuca     1920

acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc cucuucggcg     1980

gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg     2040

cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc gucuuccucg     2100

ccaccaacgu ccacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5797
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  5797
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac accugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc aagggcgccc     1860

agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc ggcgucuuca     1920

acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc cucuucggcg     1980

gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg     2040

cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc gucuuccucg     2100

ccaccaacgu ccacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5798
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 2 opt

<400>  5798
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cggcuacgag accgacgagg     1080

accgcgccaa ggucgagguc acccccaacu ccccccgcgc cgaggccacc cucggcggcu     1140

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu     1200

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1260

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1320

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1380

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1440

gccucuucuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1500

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gguccccgcc gagacccucc     1560

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagauccccg     1620

uccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1680

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1740

gcgacuccua caucgucauc ggcgucggcg acaagaagau cacccaccac uggcaccgcu     1800

ccggcuccac ccucggcaag gccuucucca ccacccucaa gggcgcccag cgccucgccg     1860

cccucggcga caccgccugg gacuucggcu ccaucggcgg cgucuucaac uccaucggcc     1920

gcgccgucca ccaggucuuc ggcggcgccu uccgcacccu cuucggcggc auguccugga     1980

ucacccaggg ccucaugggc gcccuccucc ucuggauggg cgucaacgcc cgcgaccgcu     2040

ccaucgcccu cgccuuccuc gccaccggcg gcguccucgu cuuccucgcc accaacgucc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5799
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  5799
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc aagggcgccc     1860

agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc ggcgucuuca     1920

acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc cucuucggcg     1980

gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg     2040

cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc gucuuccucg     2100

ccaccaacgu ccacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5800
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  5800
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5801
<211>  2521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5801
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg caaggagaag aagcgccgcg gcgccgacac cuccgucggc aucgucggcc      120

uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc uacuacaugu      180

accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca      240

agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug uccuacgagu      300

gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc aacaccaccu      360

ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc cgcucccgcc      420

gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc cagaccuggc      480

ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc uuccgcaacc      540

ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc accucccaga      600

aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc cgcugcaucg      660

gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc gacaucgucc      720

ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc gacaucgagc      780

ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac gaggccucca      840

ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac cucgacaagc      900

aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu      960

gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc uccaagaaga     1020

ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc uccguccacg     1080

gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac gagaaccgcg     1140

ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc ggcuucggcu     1200

cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca     1260

ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc ccccuccccu     1320

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cucgucgagu     1380

ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag gagggcgccg     1440

uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag ggccgccucu     1500

ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag ggcgucuccu     1560

acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc cuccacggca     1620

ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc cccgcccaga     1680

uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc aaccccguca     1740

ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc uucggcgacu     1800

ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac cgcuccggcu     1860

ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug gccguccucg     1920

gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc ggcaagggca     1980

uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc ugguucuccc     2040

agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac ggcuccaucu     2100

cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg     2160

acgucggcug cuccgucgac uucuccaagu gaggacuagu gcaucacauu uaaaagcauc     2220

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2280

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2340

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2460

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2520

u                                                                     2521


<210>  5802
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5802
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc gucggccucc      120

uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac uacauguacc      180

ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc augaacaagu      240

gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc uacgagugcc      300

ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac accaccucca      360

ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc ucccgccgcg      420

ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag accuggcucg      480

agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg      540

gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc ucccagaagg      600

ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc ugcaucggcg      660

ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac aucguccucg      720

agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac aucgagcucg      780

ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag gccuccaucu      840

ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc gacaagcagu      900

ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg      960

gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc aagaagauga     1020

ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc guccacggcu     1080

cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag aaccgcgcca     1140

aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc uucggcuccc     1200

ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac uaccucacca     1260

ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc cuccccuggc     1320

acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc gucgaguuca     1380

aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag ggcgccgucc     1440

acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc cgccucuccu     1500

ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc gucuccuacu     1560

cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc cacggcaccg     1620

ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc gcccagaugg     1680

ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac cccgucauca     1740

ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc ggcgacuccu     1800

acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc uccggcucca     1860

ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc guccucggcg     1920

acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc aagggcaucc     1980

accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg uucucccaga     2040

uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc uccaucuccc     2100

ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc uccgccgacg     2160

ucggcugcuc cgucgacuuc uccaagugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  5803
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5803
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cccgcaagga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc ggccuccucc      120

ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacgucg     2160

gcugcuccgu cgacuucucc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5804
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5804
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gcaaggagaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc cuccuccuca      120

ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug uaccucgacc      180

gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac aagugcuaca      240

uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag ugccccaugc      300

ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc uccaccuggg      360

ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca      420

cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg cucgaguccc      480

gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac cccggcuucg      540

cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag aaggucaucu      600

accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc ggcgucucca      660

accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc cucgagcacg      720

gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag cucgucacca      780

ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc aucuccgaca      840

uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag caguccgaca      900

cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc ugcggccucu      960

ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag augaccggca     1020

aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac ggcucccagc     1080

acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc gccaaggucg     1140

agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc     1200

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca     1260

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg     1440

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uccuccggcc     1500

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc accgucaccg     1620

ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag auggccgucg     1680

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1740

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1800

ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1860

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1920

ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc cagauccuca     2040

ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc ucccucaugu     2100

gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc gacgucggcu     2160

gcuccgucga cuucuccaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5805
<211>  2509
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5805
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

aggagaagaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc cuccucacca      120

ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac cucgaccgca      180

acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag ugcuacaucc      240

agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc cccaugcucg      300

acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc accugggucg      360

ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc gccgucaccc      420

uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc gagucccgcg      480

aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc      540

ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag gucaucuacc      600

ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc gucuccaacc      660

gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc gagcacggcg      720

gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc gucaccacca      780

ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc uccgacaugg      840

ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag uccgacaccc      900

aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc ggccucuucg      960

gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug accggcaagu     1020

ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc ucccagcacu     1080

ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc aaggucgaga     1140

ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc cucggccucg     1200

acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc augaacaaca     1260

agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg cacgccggcg     1320

ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc aaggacgccc     1380

acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc cacaccgccc     1440

ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc uccggccacc     1500

ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac ucccucugca     1560

ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc gucaccgucg     1620

agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug gccgucgaca     1680

ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc accgagucca     1740

ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc uacaucguca     1800

ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc accaucggca     1860

aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc gacaccgccu     1920

gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc caccagaucu     1980

ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag auccucaucg     2040

gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc cucaugugcc     2100

ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac gucggcugcu     2160

ccgucgacuu cuccaaguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2220

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2280

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2340

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2460

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2509


<210>  5806
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5806
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc cucaccaccg      120

ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg      180

acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc uacauccaga      240

ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc augcucgacg      300

agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc ugggucgucu      360

acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc      420

ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag ucccgcgagu      480

acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc uucgcccucg      540

ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc aucuaccucg      600

ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc uccaaccgcg      660

acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag cacggcggcu      720

gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc accaccaccg      780

ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc gacauggccu      840

ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc gacacccagu      900

acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc cucuucggca      960

agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc ggcaagucca     1020

uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc cagcacuccg     1080

gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag gucgagauca     1140

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu     1200

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1380

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1440

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca     1500

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1560

ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc accgucgagg     1620

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1680

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1740

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1800

gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1860

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1920

acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     2040

cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc augugccucg     2100

cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacguc ggcugcuccg     2160

ucgacuucuc caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5807
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5807
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagcgccg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc accaccgcca      120

uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac cgcaacgacg      180

ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac auccagauca      240

uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug cucgacgagg      300

gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg gucgucuacg      360

gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc acccuccccu      420

cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc cgcgaguaca      480

ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg      540

ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc uaccucguca      600

ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc aaccgcgacu      660

ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg      720

ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc accaccgucu      780

ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac auggccuccg      840

acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac acccaguacg      900

ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg      960

gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc aaguccaucc     1020

agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag cacuccggca     1080

ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc gagaucaccc     1140

ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc cucgacugcg     1200

agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac aacaagcacu     1260

ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca     1320

ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac gcccacgcca     1380

agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc gcccucgccg     1440

gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc caccucaagu     1500

gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc ugcaccgccg     1560

ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc gucgaggucc     1620

aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc gacaugcaga     1680

cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag uccaccgaga     1740

acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc gucaucggcg     1800

ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc ggcaaggccu     1860

ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc gccugggacu     1920

ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag aucuucggcg     1980

ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc aucggcaccc     2040

uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug ugccucgccc     2100

ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc ugcuccgucg     2160

acuucuccaa gugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  5808
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5808
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agcgccgcgg cgccgacacc uccgucggca ucgucggccu ccuccucacc accgccaugg      120

ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1920

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     2040

ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc cucgcccucg     2100

gcggcguccu caucuuccuc uccaccgccg ucuccgccga cgucggcugc uccgucgacu     2160

ucuccaagug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  5809
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5809
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

gccgcggcgc cgacaccucc gucggcaucg ucggccuccu ccucaccacc gccauggccg      120

ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag gccuucgagg     1860

ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg gacuucggcu     1920

ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc acccuccuca     2040

uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc gcccucggcg     2100

gcguccucau cuuccucucc accgccgucu ccgccgacgu cggcugcucc gucgacuucu     2160

ccaagugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5810
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5810
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

gcggcgccga caccuccguc ggcaucgucg gccuccuccu caccaccgcc auggccgccg      120

aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac gccggcgagg      180

ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc auggaccucg      240

gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag ggcgucgagc      300

ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac ggcaccugcc      360

accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc ucccacucca      420

cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac accaagcacc      480

ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc gccgccgcca      540

ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc augauccucc      600

ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac uucgucgagg      660

gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc gucaccguca      720

uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc uccaacaugg      780

ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc gacucccgcu      840

gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac gucugcaagc      900

gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag ggcucccucg      960

ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc cagcccgaga     1020

accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc augaucguca     1080

acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc cccaacuccc     1140

cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc gagccccgca     1200

ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac uggcucgucc     1260

acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac accggcaccc     1320

cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc aagcgccaga     1380

ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc ggcgcccucg     1440

aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag ugccgccuca     1500

agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc gccuucaccu     1560

ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc caguacgccg     1620

gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag acccucaccc     1680

ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag aacuccaaga     1740

ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc gucggcgaga     1800

agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc uucgaggcca     1860

ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac uucggcuccg     1920

ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc gccgccuuca     1980

agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc cuccucaugu     2040

ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc cucggcggcg     2100

uccucaucuu ccucuccacc gccgucuccg ccgacgucgg cugcuccguc gacuucucca     2160

agugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5811
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5811
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug gccgccgagg      120

ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc ggcgaggcca      180

ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug gaccucggcc      240

acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc gucgagcccg      300

acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc accugccacc      360

acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc cacuccaccc      420

gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc aagcaccuca      480

uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg      540

ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug auccuccuca      600

ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca      660

uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc accgucaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc aacauggccg      780

agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac ucccgcugcc      840

ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc ugcaagcgca      900

cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc ucccucguca      960

ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag cccgagaacc     1020

ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug aucgucaacg     1080

acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc aacucccccc     1140

gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag ccccgcaccg     1200

gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg cucguccaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag cgccagaccg     1380

ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc gcccucgagg     1440

ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc cgccucaaga     1500

uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag uacgccggca     1620

ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc cucacccccg     1680

ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac uccaagauga     1740

ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc ggcgagaaga     1800

agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg     1860

uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc ggcuccgucg     1920

gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc gccuucaagu     1980

cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc cucauguggc     2040

ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc     2100

ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cuccgucgac uucuccaagu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5812
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5812
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccuc cgucggcauc gucggccucc uccucaccac cgccauggcc gccgagguca      120

cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc gaggccaucu      180

ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac cucggccaca      240

ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc gagcccgacg      300

acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc ugccaccaca      360

agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac uccacccgca      420

agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag caccucaucc      480

gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu      540

ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc cuccucaucg      600

cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu      660

ccggcggcac cugggucgac aucguccucg agcacggcgg cugcgucacc gucauggccc      720

aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac auggccgagg      780

uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc cgcugcccca      840

cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc aagcgcaccc      900

ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc cucgucaccu      960

gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc gagaaccucg     1020

aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc gucaacgaca     1080

ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac uccccccgcg     1140

ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc cgcaccggcc     1200

ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc guccacaagg     1260

agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc cagaccgucg     1380

ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg     1440

agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc cucaagaugg     1500

acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc accuucacca     1560

agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac gccggcaccg     1620

acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc acccccgucg     1680

gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc aagaugaugc     1740

ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc gagaagaaga     1800

ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc uccgucggcg     1920

gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc uucaaguccc     1980

ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc auguggcucg     2040

gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc ggcguccuca     2100

ucuuccucuc caccgccguc uccgccgacg ucggcugcuc cgucgacuuc uccaagugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5813
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5813
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc gaggucaccc      120

gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag gccaucuccu      180

uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc ggccacaugu      240

gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag cccgacgacg      300

ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc caccacaaga      360

agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc acccgcaagc      420

uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac cucauccgcg      480

ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc      540

uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc cucaucgccc      600

ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag ggcauguccg      660

gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc auggcccagg      720

acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug gccgaggucc      780

gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc ugccccaccc      840

agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag cgcacccucg      900

ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg      960

ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag aaccucgagu     1020

accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc aacgacaccg     1080

gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg     1140

aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc accggccucg     1200

acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc cacaaggagu     1260

gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag accgucgucg     1380

uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc gaggccgaga     1440

uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc aagauggaca     1500

agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc uucaccaaga     1560

uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg     1620

gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc cccgucggcc     1680

gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag augaugcucg     1740

agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag aagaagauca     1800

cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc gucggcggcg     1920

cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc aagucccucu     1980

ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug uggcucggcc     2040

ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc guccucaucu     2100

uccucuccac cgccgucucc gccgacgucg gcugcuccgu cgacuucucc aagugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5814
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5814
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccuccgucgg caucgucggc cuccuccuca ccaccgccau ggccgccgag gucacccgcc      120

gcggcuccgc cuacuacaug uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc      180

ccaccacccu cggcaugaac aagugcuaca uccagaucau ggaccucggc cacaugugcg      240

acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc gacgacgucg      300

acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac cacaagaagg      360

gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc cgcaagcucc      420

agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc auccgcgucg      480

agaacuggau cuuccgcaac cccggcuucg cccucgccgc cgccgccauc gccuggcucc      540

ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc aucgcccccg      600

ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc auguccggcg      660

gcaccugggu cgacaucguc cucgagcacg gcggcugcgu caccgucaug gcccaggaca      720

agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc gagguccgcu      780

ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc cccacccagg      840

gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc acccucgucg      900

accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc accugcgcca      960

aguucgccug cuccaagaag augaccggca aguccaucca gcccgagaac cucgaguacc     1020

gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac gacaccggcc     1080

acgagaccga cgagaaccgc gccaaggucg agaucacccc caacuccccc cgcgccgagg     1140

ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc ggccucgacu     1200

ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac aaggaguggu     1260

uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc cacuggaaca     1320

acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc gucgucgucc     1380

ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag gccgagaugg     1440

acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag auggacaagc     1500

uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc accgacggcc     1620

ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc gucggccgcc     1680

ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug augcucgagc     1740

ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgagaag aagaucaccc     1800

accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc guccgcggcg     1860

ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc     1920

ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag ucccucuucg     1980

gcggcauguc cugguucucc cagauccuca ucggcacccu ccucaugugg cucggccuca     2040

acaccaagaa cggcuccauc ucccucaugu gccucgcccu cggcggcguc cucaucuucc     2100

ucuccaccgc cgucuccgcc gacgucggcu gcuccgucga cuucuccaag ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5815
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5815
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccgac gucggcugcu ccgucgacuu cuccaaguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5816
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5816
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccgac gucggcugcu ccgucgacuu cuccaaguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5817
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5817
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug gccgccgccg      120

ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc ggcgaggcca      180

ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug gaccucggcc      240

acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc gucgagcccg      300

acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc accugccacc      360

acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc cacuccaccc      420

gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc aagcaccuca      480

uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg      540

ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug auccuccuca      600

ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca      660

uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc accgucaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc aacauggccg      780

agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac ucccgcugcc      840

ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc ugcaagcgca      900

cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc ucccucguca      960

ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag cccgagaacc     1020

ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug aucgucaacg     1080

acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc aacucccccc     1140

gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag ccccgcaccg     1200

gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg cucguccaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag cgccagaccg     1380

ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc gcccucgagg     1440

ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc cgccucaaga     1500

uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag uacgccggca     1620

ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc cucacccccg     1680

ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac uccaagauga     1740

ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc ggcgagaaga     1800

agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg     1860

uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc ggcuccgucg     1920

gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc gccuucaagu     1980

cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc cucauguggc     2040

ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc     2100

ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cuccgucgac uucuccaagu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5818
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5818
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucuccuc ccgcaagcgc cgcucccacg acguccucac cguccaguuc cucauccucg      120

gcaugcuccu caugaccggc ggcgucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacgucg     2160

gcugcuccgu cgacuucucc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5819
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5819
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucuccuc ccgcaagcgc cgcucccacg acguccucac cguccaguuc cucauccucg      120

gcaugcuccu caugaccggc gccgucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacgucg     2160

gcugcuccgu cgacuucucc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  5820
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5820
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5821
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5821
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg caaggagaag aagcgccgcg gcgccgacac cuccgucggc aucgucggcc      120

uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc uacuacaugu      180

accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca      240

agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug uccuacgagu      300

gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc aacaccaccu      360

ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc cgcucccgcc      420

gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc cagaccuggc      480

ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc uuccgcaacc      540

ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc accucccaga      600

aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc cgcugcaucg      660

gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc gacaucgucc      720

ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc gacaucgagc      780

ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac gaggccucca      840

ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac cucgacaagc      900

aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu      960

gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc uccaagaaga     1020

ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc uccguccacg     1080

gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac gagaaccgcg     1140

ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc ggcuucggcu     1200

cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca     1260

ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc ccccuccccu     1320

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cucgucgagu     1380

ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag gagggcgccg     1440

uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag ggccgccucu     1500

ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag ggcgucuccu     1560

acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc cuccacggca     1620

ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc cccgcccaga     1680

uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc aaccccguca     1740

ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc uucggcgacu     1800

ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac cgcuccggcu     1860

ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug gccguccucg     1920

gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc ggcaagggca     1980

uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc ugguucuccc     2040

agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac ggcuccaucu     2100

cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg     2160

acgucggcug cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  5822
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5822
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc gucggccucc      120

uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac uacauguacc      180

ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc augaacaagu      240

gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc uacgagugcc      300

ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac accaccucca      360

ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc ucccgccgcg      420

ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag accuggcucg      480

agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg      540

gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc ucccagaagg      600

ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc ugcaucggcg      660

ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac aucguccucg      720

agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac aucgagcucg      780

ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag gccuccaucu      840

ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc gacaagcagu      900

ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg      960

gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc aagaagauga     1020

ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc guccacggcu     1080

cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag aaccgcgcca     1140

aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc uucggcuccc     1200

ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac uaccucacca     1260

ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc cuccccuggc     1320

acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc gucgaguuca     1380

aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag ggcgccgucc     1440

acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc cgccucuccu     1500

ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc gucuccuacu     1560

cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc cacggcaccg     1620

ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc gcccagaugg     1680

ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac cccgucauca     1740

ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc ggcgacuccu     1800

acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc uccggcucca     1860

ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc guccucggcg     1920

acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc aagggcaucc     1980

accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg uucucccaga     2040

uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc uccaucuccc     2100

ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc uccgccgacg     2160

ucggcugcug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  5823
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5823
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cccgcaagga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc ggccuccucc      120

ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacgucg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5824
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5824
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gcaaggagaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc cuccuccuca      120

ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug uaccucgacc      180

gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac aagugcuaca      240

uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag ugccccaugc      300

ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc uccaccuggg      360

ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca      420

cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg cucgaguccc      480

gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac cccggcuucg      540

cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag aaggucaucu      600

accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc ggcgucucca      660

accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc cucgagcacg      720

gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag cucgucacca      780

ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc aucuccgaca      840

uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag caguccgaca      900

cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc ugcggccucu      960

ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag augaccggca     1020

aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac ggcucccagc     1080

acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc gccaaggucg     1140

agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc     1200

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca     1260

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg     1440

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uccuccggcc     1500

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc accgucaccg     1620

ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag auggccgucg     1680

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1740

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1800

ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1860

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1920

ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc cagauccuca     2040

ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc ucccucaugu     2100

gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc gacgucggcu     2160

gcugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5825
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5825
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

aggagaagaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc cuccucacca      120

ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac cucgaccgca      180

acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag ugcuacaucc      240

agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc cccaugcucg      300

acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc accugggucg      360

ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc gccgucaccc      420

uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc gagucccgcg      480

aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc      540

ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag gucaucuacc      600

ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc gucuccaacc      660

gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc gagcacggcg      720

gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc gucaccacca      780

ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc uccgacaugg      840

ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag uccgacaccc      900

aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc ggccucuucg      960

gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug accggcaagu     1020

ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc ucccagcacu     1080

ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc aaggucgaga     1140

ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc cucggccucg     1200

acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc augaacaaca     1260

agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg cacgccggcg     1320

ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc aaggacgccc     1380

acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc cacaccgccc     1440

ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc uccggccacc     1500

ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac ucccucugca     1560

ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc gucaccgucg     1620

agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug gccgucgaca     1680

ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc accgagucca     1740

ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc uacaucguca     1800

ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc accaucggca     1860

aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc gacaccgccu     1920

gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc caccagaucu     1980

ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag auccucaucg     2040

gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc cucaugugcc     2100

ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac gucggcugcu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5826
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5826
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc cucaccaccg      120

ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg      180

acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc uacauccaga      240

ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc augcucgacg      300

agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc ugggucgucu      360

acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc      420

ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag ucccgcgagu      480

acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc uucgcccucg      540

ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc aucuaccucg      600

ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc uccaaccgcg      660

acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag cacggcggcu      720

gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc accaccaccg      780

ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc gacauggccu      840

ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc gacacccagu      900

acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc cucuucggca      960

agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc ggcaagucca     1020

uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc cagcacuccg     1080

gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag gucgagauca     1140

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu     1200

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1380

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1440

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca     1500

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1560

ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc accgucgagg     1620

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1680

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1740

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1800

gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1860

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1920

acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     2040

cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc augugccucg     2100

cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacguc ggcugcugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5827
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5827
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagcgccg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc accaccgcca      120

uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac cgcaacgacg      180

ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac auccagauca      240

uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug cucgacgagg      300

gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg gucgucuacg      360

gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc acccuccccu      420

cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc cgcgaguaca      480

ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg      540

ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc uaccucguca      600

ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc aaccgcgacu      660

ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg      720

ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc accaccgucu      780

ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac auggccuccg      840

acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac acccaguacg      900

ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg      960

gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc aaguccaucc     1020

agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag cacuccggca     1080

ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc gagaucaccc     1140

ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc cucgacugcg     1200

agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac aacaagcacu     1260

ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca     1320

ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac gcccacgcca     1380

agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc gcccucgccg     1440

gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc caccucaagu     1500

gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc ugcaccgccg     1560

ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc gucgaggucc     1620

aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc gacaugcaga     1680

cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag uccaccgaga     1740

acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc gucaucggcg     1800

ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc ggcaaggccu     1860

ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc gccugggacu     1920

ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag aucuucggcg     1980

ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc aucggcaccc     2040

uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug ugccucgccc     2100

ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc ugcugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5828
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5828
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agcgccgcgg cgccgacacc uccgucggca ucgucggccu ccuccucacc accgccaugg      120

ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1920

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     2040

ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc cucgcccucg     2100

gcggcguccu caucuuccuc uccaccgccg ucuccgccga cgucggcugc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5829
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5829
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

gccgcggcgc cgacaccucc gucggcaucg ucggccuccu ccucaccacc gccauggccg      120

ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag gccuucgagg     1860

ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg gacuucggcu     1920

ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc acccuccuca     2040

uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc gcccucggcg     2100

gcguccucau cuuccucucc accgccgucu ccgccgacgu cggcugcuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5830
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5830
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

gcggcgccga caccuccguc ggcaucgucg gccuccuccu caccaccgcc auggccgccg      120

aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac gccggcgagg      180

ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc auggaccucg      240

gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag ggcgucgagc      300

ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac ggcaccugcc      360

accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc ucccacucca      420

cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac accaagcacc      480

ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc gccgccgcca      540

ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc augauccucc      600

ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac uucgucgagg      660

gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc gucaccguca      720

uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc uccaacaugg      780

ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc gacucccgcu      840

gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac gucugcaagc      900

gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag ggcucccucg      960

ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc cagcccgaga     1020

accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc augaucguca     1080

acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc cccaacuccc     1140

cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc gagccccgca     1200

ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac uggcucgucc     1260

acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac accggcaccc     1320

cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc aagcgccaga     1380

ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc ggcgcccucg     1440

aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag ugccgccuca     1500

agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc gccuucaccu     1560

ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc caguacgccg     1620

gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag acccucaccc     1680

ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag aacuccaaga     1740

ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc gucggcgaga     1800

agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc uucgaggcca     1860

ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac uucggcuccg     1920

ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc gccgccuuca     1980

agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc cuccucaugu     2040

ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc cucggcggcg     2100

uccucaucuu ccucuccacc gccgucuccg ccgacgucgg cugcugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5831
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5831
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug gccgccgagg      120

ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc ggcgaggcca      180

ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug gaccucggcc      240

acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc gucgagcccg      300

acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc accugccacc      360

acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc cacuccaccc      420

gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc aagcaccuca      480

uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg      540

ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug auccuccuca      600

ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca      660

uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc accgucaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc aacauggccg      780

agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac ucccgcugcc      840

ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc ugcaagcgca      900

cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc ucccucguca      960

ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag cccgagaacc     1020

ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug aucgucaacg     1080

acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc aacucccccc     1140

gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag ccccgcaccg     1200

gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg cucguccaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag cgccagaccg     1380

ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc gcccucgagg     1440

ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc cgccucaaga     1500

uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag uacgccggca     1620

ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc cucacccccg     1680

ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac uccaagauga     1740

ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc ggcgagaaga     1800

agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg     1860

uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc ggcuccgucg     1920

gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc gccuucaagu     1980

cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc cucauguggc     2040

ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc     2100

ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5832
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5832
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccuc cgucggcauc gucggccucc uccucaccac cgccauggcc gccgagguca      120

cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc gaggccaucu      180

ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac cucggccaca      240

ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc gagcccgacg      300

acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc ugccaccaca      360

agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac uccacccgca      420

agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag caccucaucc      480

gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu      540

ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc cuccucaucg      600

cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu      660

ccggcggcac cugggucgac aucguccucg agcacggcgg cugcgucacc gucauggccc      720

aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac auggccgagg      780

uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc cgcugcccca      840

cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc aagcgcaccc      900

ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc cucgucaccu      960

gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc gagaaccucg     1020

aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc gucaacgaca     1080

ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac uccccccgcg     1140

ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc cgcaccggcc     1200

ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc guccacaagg     1260

agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc cagaccgucg     1380

ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg     1440

agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc cucaagaugg     1500

acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc accuucacca     1560

agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac gccggcaccg     1620

acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc acccccgucg     1680

gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc aagaugaugc     1740

ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc gagaagaaga     1800

ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc uccgucggcg     1920

gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc uucaaguccc     1980

ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc auguggcucg     2040

gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc ggcguccuca     2100

ucuuccucuc caccgccguc uccgccgacg ucggcugcug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5833
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5833
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc gaggucaccc      120

gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag gccaucuccu      180

uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc ggccacaugu      240

gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag cccgacgacg      300

ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc caccacaaga      360

agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc acccgcaagc      420

uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac cucauccgcg      480

ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc      540

uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc cucaucgccc      600

ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag ggcauguccg      660

gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc auggcccagg      720

acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug gccgaggucc      780

gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc ugccccaccc      840

agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag cgcacccucg      900

ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg      960

ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag aaccucgagu     1020

accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc aacgacaccg     1080

gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg     1140

aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc accggccucg     1200

acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc cacaaggagu     1260

gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag accgucgucg     1380

uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc gaggccgaga     1440

uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc aagauggaca     1500

agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc uucaccaaga     1560

uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg     1620

gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc cccgucggcc     1680

gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag augaugcucg     1740

agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag aagaagauca     1800

cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc gucggcggcg     1920

cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc aagucccucu     1980

ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug uggcucggcc     2040

ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc guccucaucu     2100

uccucuccac cgccgucucc gccgacgucg gcugcugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5834
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5834
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccuccgucgg caucgucggc cuccuccuca ccaccgccau ggccgccgag gucacccgcc      120

gcggcuccgc cuacuacaug uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc      180

ccaccacccu cggcaugaac aagugcuaca uccagaucau ggaccucggc cacaugugcg      240

acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc gacgacgucg      300

acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac cacaagaagg      360

gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc cgcaagcucc      420

agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc auccgcgucg      480

agaacuggau cuuccgcaac cccggcuucg cccucgccgc cgccgccauc gccuggcucc      540

ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc aucgcccccg      600

ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc auguccggcg      660

gcaccugggu cgacaucguc cucgagcacg gcggcugcgu caccgucaug gcccaggaca      720

agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc gagguccgcu      780

ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc cccacccagg      840

gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc acccucgucg      900

accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc accugcgcca      960

aguucgccug cuccaagaag augaccggca aguccaucca gcccgagaac cucgaguacc     1020

gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac gacaccggcc     1080

acgagaccga cgagaaccgc gccaaggucg agaucacccc caacuccccc cgcgccgagg     1140

ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc ggccucgacu     1200

ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac aaggaguggu     1260

uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc cacuggaaca     1320

acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc gucgucgucc     1380

ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag gccgagaugg     1440

acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag auggacaagc     1500

uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc accgacggcc     1620

ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc gucggccgcc     1680

ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug augcucgagc     1740

ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgagaag aagaucaccc     1800

accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc guccgcggcg     1860

ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc     1920

ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag ucccucuucg     1980

gcggcauguc cugguucucc cagauccuca ucggcacccu ccucaugugg cucggccuca     2040

acaccaagaa cggcuccauc ucccucaugu gccucgcccu cggcggcguc cucaucuucc     2100

ucuccaccgc cgucuccgcc gacgucggcu gcugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5835
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5835
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccgac gucggcugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5836
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5836
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccgac gucggcugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5837
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5837
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug gccgccgccg      120

ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc ggcgaggcca      180

ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug gaccucggcc      240

acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc gucgagcccg      300

acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc accugccacc      360

acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc cacuccaccc      420

gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc aagcaccuca      480

uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg      540

ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug auccuccuca      600

ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca      660

uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc accgucaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc aacauggccg      780

agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac ucccgcugcc      840

ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc ugcaagcgca      900

cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc ucccucguca      960

ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag cccgagaacc     1020

ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug aucgucaacg     1080

acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc aacucccccc     1140

gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag ccccgcaccg     1200

gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg cucguccaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag cgccagaccg     1380

ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc gcccucgagg     1440

ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc cgccucaaga     1500

uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag uacgccggca     1620

ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc cucacccccg     1680

ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac uccaagauga     1740

ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc ggcgagaaga     1800

agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg     1860

uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc ggcuccgucg     1920

gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc gccuucaagu     1980

cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc cucauguggc     2040

ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc     2100

ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5838
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5838
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucuccuc ccgcaagcgc cgcucccacg acguccucac cguccaguuc cucauccucg      120

gcaugcuccu caugaccggc ggcgucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacgucg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5839
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5839
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucuccuc ccgcaagcgc cgcucccacg acguccucac cguccaguuc cucauccucg      120

gcaugcuccu caugaccggc gccgucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacgucg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5840
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5840
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  5841
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5841
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg caaggagaag aagcgccgcg gcgccgacac cuccgucggc aucgucggcc      120

uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc uacuacaugu      180

accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca      240

agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug uccuacgagu      300

gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc aacaccaccu      360

ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc cgcucccgcc      420

gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc cagaccuggc      480

ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc uuccgcaacc      540

ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc accucccaga      600

aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc cgcugcaucg      660

gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc gacaucgucc      720

ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc gacaucgagc      780

ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac gaggccucca      840

ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac cucgacaagc      900

aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu      960

gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc uccaagaaga     1020

ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc uccguccacg     1080

gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac gagaaccgcg     1140

ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc ggcuucggcu     1200

cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca     1260

ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc ccccuccccu     1320

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cucgucgagu     1380

ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag gagggcgccg     1440

uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag ggccgccucu     1500

ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag ggcgucuccu     1560

acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc cuccacggca     1620

ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc cccgcccaga     1680

uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc aaccccguca     1740

ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc uucggcgacu     1800

ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac cgcuccggcu     1860

ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug gccguccucg     1920

gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc ggcaagggca     1980

uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc ugguucuccc     2040

agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac ggcuccaucu     2100

cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg     2160

acugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5842
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5842
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc gucggccucc      120

uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac uacauguacc      180

ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc augaacaagu      240

gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc uacgagugcc      300

ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac accaccucca      360

ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc ucccgccgcg      420

ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag accuggcucg      480

agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg      540

gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc ucccagaagg      600

ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc ugcaucggcg      660

ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac aucguccucg      720

agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac aucgagcucg      780

ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag gccuccaucu      840

ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc gacaagcagu      900

ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg      960

gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc aagaagauga     1020

ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc guccacggcu     1080

cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag aaccgcgcca     1140

aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc uucggcuccc     1200

ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac uaccucacca     1260

ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc cuccccuggc     1320

acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc gucgaguuca     1380

aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag ggcgccgucc     1440

acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc cgccucuccu     1500

ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc gucuccuacu     1560

cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc cacggcaccg     1620

ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc gcccagaugg     1680

ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac cccgucauca     1740

ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc ggcgacuccu     1800

acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc uccggcucca     1860

ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc guccucggcg     1920

acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc aagggcaucc     1980

accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg uucucccaga     2040

uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc uccaucuccc     2100

ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc uccgccgacu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5843
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5843
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cccgcaagga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc ggccuccucc      120

ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5844
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5844
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gcaaggagaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc cuccuccuca      120

ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug uaccucgacc      180

gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac aagugcuaca      240

uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag ugccccaugc      300

ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc uccaccuggg      360

ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca      420

cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg cucgaguccc      480

gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac cccggcuucg      540

cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag aaggucaucu      600

accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc ggcgucucca      660

accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc cucgagcacg      720

gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag cucgucacca      780

ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc aucuccgaca      840

uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag caguccgaca      900

cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc ugcggccucu      960

ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag augaccggca     1020

aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac ggcucccagc     1080

acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc gccaaggucg     1140

agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc     1200

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca     1260

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg     1440

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uccuccggcc     1500

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc accgucaccg     1620

ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag auggccgucg     1680

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1740

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1800

ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1860

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1920

ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc cagauccuca     2040

ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc ucccucaugu     2100

gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc gacugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5845
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5845
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

aggagaagaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc cuccucacca      120

ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac cucgaccgca      180

acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag ugcuacaucc      240

agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc cccaugcucg      300

acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc accugggucg      360

ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc gccgucaccc      420

uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc gagucccgcg      480

aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc      540

ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag gucaucuacc      600

ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc gucuccaacc      660

gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc gagcacggcg      720

gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc gucaccacca      780

ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc uccgacaugg      840

ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag uccgacaccc      900

aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc ggccucuucg      960

gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug accggcaagu     1020

ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc ucccagcacu     1080

ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc aaggucgaga     1140

ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc cucggccucg     1200

acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc augaacaaca     1260

agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg cacgccggcg     1320

ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc aaggacgccc     1380

acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc cacaccgccc     1440

ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc uccggccacc     1500

ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac ucccucugca     1560

ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc gucaccgucg     1620

agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug gccgucgaca     1680

ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc accgagucca     1740

ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc uacaucguca     1800

ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc accaucggca     1860

aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc gacaccgccu     1920

gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc caccagaucu     1980

ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag auccucaucg     2040

gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc cucaugugcc     2100

ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5846
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5846
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc cucaccaccg      120

ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg      180

acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc uacauccaga      240

ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc augcucgacg      300

agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc ugggucgucu      360

acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc      420

ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag ucccgcgagu      480

acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc uucgcccucg      540

ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc aucuaccucg      600

ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc uccaaccgcg      660

acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag cacggcggcu      720

gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc accaccaccg      780

ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc gacauggccu      840

ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc gacacccagu      900

acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc cucuucggca      960

agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc ggcaagucca     1020

uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc cagcacuccg     1080

gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag gucgagauca     1140

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu     1200

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1380

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1440

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca     1500

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1560

ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc accgucgagg     1620

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1680

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1740

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1800

gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1860

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1920

acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     2040

cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc augugccucg     2100

cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5847
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5847
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagcgccg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc accaccgcca      120

uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac cgcaacgacg      180

ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac auccagauca      240

uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug cucgacgagg      300

gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg gucgucuacg      360

gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc acccuccccu      420

cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc cgcgaguaca      480

ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg      540

ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc uaccucguca      600

ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc aaccgcgacu      660

ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg      720

ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc accaccgucu      780

ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac auggccuccg      840

acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac acccaguacg      900

ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg      960

gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc aaguccaucc     1020

agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag cacuccggca     1080

ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc gagaucaccc     1140

ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc cucgacugcg     1200

agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac aacaagcacu     1260

ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca     1320

ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac gcccacgcca     1380

agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc gcccucgccg     1440

gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc caccucaagu     1500

gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc ugcaccgccg     1560

ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc gucgaggucc     1620

aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc gacaugcaga     1680

cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag uccaccgaga     1740

acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc gucaucggcg     1800

ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc ggcaaggccu     1860

ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc gccugggacu     1920

ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag aucuucggcg     1980

ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc aucggcaccc     2040

uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug ugccucgccc     2100

ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5848
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5848
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agcgccgcgg cgccgacacc uccgucggca ucgucggccu ccuccucacc accgccaugg      120

ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1920

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     2040

ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc cucgcccucg     2100

gcggcguccu caucuuccuc uccaccgccg ucuccgccga cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5849
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5849
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

gccgcggcgc cgacaccucc gucggcaucg ucggccuccu ccucaccacc gccauggccg      120

ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag gccuucgagg     1860

ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg gacuucggcu     1920

ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc acccuccuca     2040

uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc gcccucggcg     2100

gcguccucau cuuccucucc accgccgucu ccgccgacug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5850
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5850
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

gcggcgccga caccuccguc ggcaucgucg gccuccuccu caccaccgcc auggccgccg      120

aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac gccggcgagg      180

ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc auggaccucg      240

gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag ggcgucgagc      300

ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac ggcaccugcc      360

accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc ucccacucca      420

cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac accaagcacc      480

ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc gccgccgcca      540

ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc augauccucc      600

ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac uucgucgagg      660

gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc gucaccguca      720

uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc uccaacaugg      780

ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc gacucccgcu      840

gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac gucugcaagc      900

gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag ggcucccucg      960

ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc cagcccgaga     1020

accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc augaucguca     1080

acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc cccaacuccc     1140

cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc gagccccgca     1200

ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac uggcucgucc     1260

acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac accggcaccc     1320

cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc aagcgccaga     1380

ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc ggcgcccucg     1440

aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag ugccgccuca     1500

agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc gccuucaccu     1560

ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc caguacgccg     1620

gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag acccucaccc     1680

ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag aacuccaaga     1740

ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc gucggcgaga     1800

agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc uucgaggcca     1860

ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac uucggcuccg     1920

ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc gccgccuuca     1980

agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc cuccucaugu     2040

ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc cucggcggcg     2100

uccucaucuu ccucuccacc gccgucuccg ccgacugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5851
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5851
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug gccgccgagg      120

ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc ggcgaggcca      180

ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug gaccucggcc      240

acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc gucgagcccg      300

acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc accugccacc      360

acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc cacuccaccc      420

gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc aagcaccuca      480

uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg      540

ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug auccuccuca      600

ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca      660

uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc accgucaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc aacauggccg      780

agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac ucccgcugcc      840

ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc ugcaagcgca      900

cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc ucccucguca      960

ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag cccgagaacc     1020

ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug aucgucaacg     1080

acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc aacucccccc     1140

gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag ccccgcaccg     1200

gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg cucguccaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag cgccagaccg     1380

ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc gcccucgagg     1440

ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc cgccucaaga     1500

uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag uacgccggca     1620

ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc cucacccccg     1680

ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac uccaagauga     1740

ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc ggcgagaaga     1800

agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg     1860

uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc ggcuccgucg     1920

gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc gccuucaagu     1980

cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc cucauguggc     2040

ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc     2100

ucaucuuccu cuccaccgcc gucuccgccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5852
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5852
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccuc cgucggcauc gucggccucc uccucaccac cgccauggcc gccgagguca      120

cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc gaggccaucu      180

ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac cucggccaca      240

ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc gagcccgacg      300

acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc ugccaccaca      360

agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac uccacccgca      420

agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag caccucaucc      480

gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu      540

ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc cuccucaucg      600

cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu      660

ccggcggcac cugggucgac aucguccucg agcacggcgg cugcgucacc gucauggccc      720

aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac auggccgagg      780

uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc cgcugcccca      840

cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc aagcgcaccc      900

ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc cucgucaccu      960

gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc gagaaccucg     1020

aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc gucaacgaca     1080

ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac uccccccgcg     1140

ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc cgcaccggcc     1200

ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc guccacaagg     1260

agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc cagaccgucg     1380

ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg     1440

agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc cucaagaugg     1500

acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc accuucacca     1560

agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac gccggcaccg     1620

acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc acccccgucg     1680

gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc aagaugaugc     1740

ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc gagaagaaga     1800

ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc uccgucggcg     1920

gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc uucaaguccc     1980

ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc auguggcucg     2040

gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc ggcguccuca     2100

ucuuccucuc caccgccguc uccgccgacu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5853
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5853
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc gaggucaccc      120

gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag gccaucuccu      180

uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc ggccacaugu      240

gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag cccgacgacg      300

ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc caccacaaga      360

agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc acccgcaagc      420

uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac cucauccgcg      480

ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc      540

uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc cucaucgccc      600

ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag ggcauguccg      660

gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc auggcccagg      720

acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug gccgaggucc      780

gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc ugccccaccc      840

agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag cgcacccucg      900

ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg      960

ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag aaccucgagu     1020

accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc aacgacaccg     1080

gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg     1140

aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc accggccucg     1200

acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc cacaaggagu     1260

gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag accgucgucg     1380

uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc gaggccgaga     1440

uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc aagauggaca     1500

agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc uucaccaaga     1560

uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg     1620

gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc cccgucggcc     1680

gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag augaugcucg     1740

agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag aagaagauca     1800

cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc gucggcggcg     1920

cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc aagucccucu     1980

ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug uggcucggcc     2040

ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc guccucaucu     2100

uccucuccac cgccgucucc gccgacugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  5854
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5854
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccuccgucgg caucgucggc cuccuccuca ccaccgccau ggccgccgag gucacccgcc      120

gcggcuccgc cuacuacaug uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc      180

ccaccacccu cggcaugaac aagugcuaca uccagaucau ggaccucggc cacaugugcg      240

acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc gacgacgucg      300

acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac cacaagaagg      360

gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc cgcaagcucc      420

agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc auccgcgucg      480

agaacuggau cuuccgcaac cccggcuucg cccucgccgc cgccgccauc gccuggcucc      540

ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc aucgcccccg      600

ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc auguccggcg      660

gcaccugggu cgacaucguc cucgagcacg gcggcugcgu caccgucaug gcccaggaca      720

agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc gagguccgcu      780

ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc cccacccagg      840

gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc acccucgucg      900

accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc accugcgcca      960

aguucgccug cuccaagaag augaccggca aguccaucca gcccgagaac cucgaguacc     1020

gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac gacaccggcc     1080

acgagaccga cgagaaccgc gccaaggucg agaucacccc caacuccccc cgcgccgagg     1140

ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc ggccucgacu     1200

ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac aaggaguggu     1260

uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc cacuggaaca     1320

acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc gucgucgucc     1380

ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag gccgagaugg     1440

acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag auggacaagc     1500

uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc accgacggcc     1620

ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc gucggccgcc     1680

ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug augcucgagc     1740

ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgagaag aagaucaccc     1800

accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc guccgcggcg     1860

ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc     1920

ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag ucccucuucg     1980

gcggcauguc cugguucucc cagauccuca ucggcacccu ccucaugugg cucggccuca     2040

acaccaagaa cggcuccauc ucccucaugu gccucgcccu cggcggcguc cucaucuucc     2100

ucuccaccgc cgucuccgcc gacugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  5855
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5855
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccgac ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5856
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5856
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccgac ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5857
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5857
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug gccgccgccg      120

ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc ggcgaggcca      180

ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug gaccucggcc      240

acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc gucgagcccg      300

acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc accugccacc      360

acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc cacuccaccc      420

gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc aagcaccuca      480

uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg      540

ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug auccuccuca      600

ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca      660

uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc accgucaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc aacauggccg      780

agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac ucccgcugcc      840

ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc ugcaagcgca      900

cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc ucccucguca      960

ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag cccgagaacc     1020

ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug aucgucaacg     1080

acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc aacucccccc     1140

gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag ccccgcaccg     1200

gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg cucguccaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag cgccagaccg     1380

ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc gcccucgagg     1440

ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc cgccucaaga     1500

uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag uacgccggca     1620

ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc cucacccccg     1680

ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac uccaagauga     1740

ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc ggcgagaaga     1800

agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg     1860

uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc ggcuccgucg     1920

gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc gccuucaagu     1980

cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc cucauguggc     2040

ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc     2100

ucaucuuccu cuccaccgcc gucuccgccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5858
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5858
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucuccuc ccgcaagcgc cgcucccacg acguccucac cguccaguuc cucauccucg      120

gcaugcuccu caugaccggc ggcgucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5859
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5859
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucuccuc ccgcaagcgc cgcucccacg acguccucac cguccaguuc cucauccucg      120

gcaugcuccu caugaccggc gccgucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5860
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5860
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  5861
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5861
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc gucggccucc      120

uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac uacauguacc      180

ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc augaacaagu      240

gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc uacgagugcc      300

ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac accaccucca      360

ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc ucccgccgcg      420

ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag accuggcucg      480

agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg      540

gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc ucccagaagg      600

ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc ugcaucggcg      660

ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac aucguccucg      720

agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac aucgagcucg      780

ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag gccuccaucu      840

ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc gacaagcagu      900

ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg      960

gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc aagaagauga     1020

ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc guccacggcu     1080

cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag aaccgcgcca     1140

aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc uucggcuccc     1200

ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac uaccucacca     1260

ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc cuccccuggc     1320

acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc gucgaguuca     1380

aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag ggcgccgucc     1440

acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc cgccucuccu     1500

ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc gucuccuacu     1560

cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc cacggcaccg     1620

ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc gcccagaugg     1680

ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac cccgucauca     1740

ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc ggcgacuccu     1800

acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc uccggcucca     1860

ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc guccucggcg     1920

acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc aagggcaucc     1980

accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg uucucccaga     2040

uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc uccaucuccc     2100

ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc uccgccugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  5862
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5862
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg caaggagaag aagcgccgcg gcgccgacac cuccgucggc aucgucggcc      120

uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc uacuacaugu      180

accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca      240

agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug uccuacgagu      300

gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc aacaccaccu      360

ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc cgcucccgcc      420

gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc cagaccuggc      480

ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc uuccgcaacc      540

ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc accucccaga      600

aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc cgcugcaucg      660

gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc gacaucgucc      720

ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc gacaucgagc      780

ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac gaggccucca      840

ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac cucgacaagc      900

aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu      960

gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc uccaagaaga     1020

ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc uccguccacg     1080

gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac gagaaccgcg     1140

ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc ggcuucggcu     1200

cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca     1260

ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc ccccuccccu     1320

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cucgucgagu     1380

ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag gagggcgccg     1440

uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag ggccgccucu     1500

ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag ggcgucuccu     1560

acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc cuccacggca     1620

ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc cccgcccaga     1680

uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc aaccccguca     1740

ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc uucggcgacu     1800

ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac cgcuccggcu     1860

ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug gccguccucg     1920

gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc ggcaagggca     1980

uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc ugguucuccc     2040

agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac ggcuccaucu     2100

cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  5863
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5863
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cccgcaagga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc ggccuccucc      120

ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5864
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5864
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gcaaggagaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc cuccuccuca      120

ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug uaccucgacc      180

gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac aagugcuaca      240

uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag ugccccaugc      300

ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc uccaccuggg      360

ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc cgcgccguca      420

cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg cucgaguccc      480

gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac cccggcuucg      540

cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag aaggucaucu      600

accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc ggcgucucca      660

accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc cucgagcacg      720

gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag cucgucacca      780

ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc aucuccgaca      840

uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag caguccgaca      900

cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc ugcggccucu      960

ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag augaccggca     1020

aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac ggcucccagc     1080

acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc gccaaggucg     1140

agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc     1200

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca     1260

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg     1440

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uccuccggcc     1500

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc accgucaccg     1620

ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag auggccgucg     1680

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1740

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1800

ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1860

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1920

ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc cagauccuca     2040

ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc ucccucaugu     2100

gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  5865
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5865
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

aggagaagaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc cuccucacca      120

ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac cucgaccgca      180

acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag ugcuacaucc      240

agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc cccaugcucg      300

acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc accugggucg      360

ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc gccgucaccc      420

uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc gagucccgcg      480

aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc      540

ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag gucaucuacc      600

ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc gucuccaacc      660

gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc gagcacggcg      720

gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc gucaccacca      780

ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc uccgacaugg      840

ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag uccgacaccc      900

aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc ggccucuucg      960

gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug accggcaagu     1020

ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc ucccagcacu     1080

ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc aaggucgaga     1140

ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc cucggccucg     1200

acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc augaacaaca     1260

agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg cacgccggcg     1320

ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc aaggacgccc     1380

acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc cacaccgccc     1440

ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc uccggccacc     1500

ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac ucccucugca     1560

ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc gucaccgucg     1620

agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug gccgucgaca     1680

ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc accgagucca     1740

ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc uacaucguca     1800

ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc accaucggca     1860

aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc gacaccgccu     1920

gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc caccagaucu     1980

ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag auccucaucg     2040

gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc cucaugugcc     2100

ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  5866
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5866
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc cucaccaccg      120

ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg      180

acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc uacauccaga      240

ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc augcucgacg      300

agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc ugggucgucu      360

acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc      420

ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag ucccgcgagu      480

acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc uucgcccucg      540

ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc aucuaccucg      600

ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc uccaaccgcg      660

acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag cacggcggcu      720

gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc accaccaccg      780

ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc gacauggccu      840

ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc gacacccagu      900

acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc cucuucggca      960

agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc ggcaagucca     1020

uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc cagcacuccg     1080

gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag gucgagauca     1140

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu     1200

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1380

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1440

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca     1500

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1560

ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc accgucgagg     1620

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1680

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1740

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1800

gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1860

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1920

acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     2040

cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc augugccucg     2100

cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  5867
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5867
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagcgccg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc accaccgcca      120

uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac cgcaacgacg      180

ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac auccagauca      240

uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug cucgacgagg      300

gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg gucgucuacg      360

gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc acccuccccu      420

cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc cgcgaguaca      480

ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg      540

ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc uaccucguca      600

ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc aaccgcgacu      660

ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg      720

ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc accaccgucu      780

ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac auggccuccg      840

acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac acccaguacg      900

ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg      960

gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc aaguccaucc     1020

agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag cacuccggca     1080

ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc gagaucaccc     1140

ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc cucgacugcg     1200

agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac aacaagcacu     1260

ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca     1320

ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac gcccacgcca     1380

agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc gcccucgccg     1440

gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc caccucaagu     1500

gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc ugcaccgccg     1560

ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc gucgaggucc     1620

aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc gacaugcaga     1680

cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag uccaccgaga     1740

acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc gucaucggcg     1800

ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc ggcaaggccu     1860

ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc gccugggacu     1920

ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag aucuucggcg     1980

ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc aucggcaccc     2040

uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug ugccucgccc     2100

ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  5868
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5868
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agcgccgcgg cgccgacacc uccgucggca ucgucggccu ccuccucacc accgccaugg      120

ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1920

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     2040

ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc cucgcccucg     2100

gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  5869
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5869
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

gccgcggcgc cgacaccucc gucggcaucg ucggccuccu ccucaccacc gccauggccg      120

ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag gccuucgagg     1860

ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg gacuucggcu     1920

ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc acccuccuca     2040

uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc gcccucggcg     2100

gcguccucau cuuccucucc accgccgucu ccgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  5870
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5870
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

gcggcgccga caccuccguc ggcaucgucg gccuccuccu caccaccgcc auggccgccg      120

aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac gccggcgagg      180

ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc auggaccucg      240

gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag ggcgucgagc      300

ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac ggcaccugcc      360

accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc ucccacucca      420

cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac accaagcacc      480

ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc gccgccgcca      540

ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc augauccucc      600

ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac uucgucgagg      660

gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc gucaccguca      720

uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc uccaacaugg      780

ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc gacucccgcu      840

gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac gucugcaagc      900

gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag ggcucccucg      960

ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc cagcccgaga     1020

accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc augaucguca     1080

acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc cccaacuccc     1140

cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc gagccccgca     1200

ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac uggcucgucc     1260

acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac accggcaccc     1320

cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc aagcgccaga     1380

ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc ggcgcccucg     1440

aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag ugccgccuca     1500

agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc gccuucaccu     1560

ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc caguacgccg     1620

gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag acccucaccc     1680

ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag aacuccaaga     1740

ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc gucggcgaga     1800

agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc uucgaggcca     1860

ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac uucggcuccg     1920

ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc gccgccuuca     1980

agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc cuccucaugu     2040

ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc cucggcggcg     2100

uccucaucuu ccucuccacc gccgucuccg ccugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  5871
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5871
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug gccgccgagg      120

ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc ggcgaggcca      180

ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug gaccucggcc      240

acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc gucgagcccg      300

acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc accugccacc      360

acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc cacuccaccc      420

gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc aagcaccuca      480

uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg      540

ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug auccuccuca      600

ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca      660

uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc accgucaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc aacauggccg      780

agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac ucccgcugcc      840

ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc ugcaagcgca      900

cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc ucccucguca      960

ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag cccgagaacc     1020

ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug aucgucaacg     1080

acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc aacucccccc     1140

gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag ccccgcaccg     1200

gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg cucguccaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag cgccagaccg     1380

ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc gcccucgagg     1440

ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc cgccucaaga     1500

uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag uacgccggca     1620

ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc cucacccccg     1680

ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac uccaagauga     1740

ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc ggcgagaaga     1800

agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg     1860

uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc ggcuccgucg     1920

gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc gccuucaagu     1980

cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc cucauguggc     2040

ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc     2100

ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5872
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5872
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccuc cgucggcauc gucggccucc uccucaccac cgccauggcc gccgagguca      120

cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc gaggccaucu      180

ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac cucggccaca      240

ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc gagcccgacg      300

acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc ugccaccaca      360

agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac uccacccgca      420

agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag caccucaucc      480

gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu      540

ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc cuccucaucg      600

cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu      660

ccggcggcac cugggucgac aucguccucg agcacggcgg cugcgucacc gucauggccc      720

aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac auggccgagg      780

uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc cgcugcccca      840

cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc aagcgcaccc      900

ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc cucgucaccu      960

gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc gagaaccucg     1020

aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc gucaacgaca     1080

ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac uccccccgcg     1140

ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc cgcaccggcc     1200

ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc guccacaagg     1260

agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc cagaccgucg     1380

ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg     1440

agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc cucaagaugg     1500

acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc accuucacca     1560

agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac gccggcaccg     1620

acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc acccccgucg     1680

gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc aagaugaugc     1740

ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc gagaagaaga     1800

ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc uccgucggcg     1920

gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc uucaaguccc     1980

ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc auguggcucg     2040

gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc ggcguccuca     2100

ucuuccucuc caccgccguc uccgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  5873
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5873
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccuccgu cggcaucguc ggccuccucc ucaccaccgc cauggccgcc gaggucaccc      120

gccgcggcuc cgccuacuac auguaccucg accgcaacga cgccggcgag gccaucuccu      180

uccccaccac ccucggcaug aacaagugcu acauccagau cauggaccuc ggccacaugu      240

gcgacgccac cauguccuac gagugcccca ugcucgacga gggcgucgag cccgacgacg      300

ucgacugcug gugcaacacc accuccaccu gggucgucua cggcaccugc caccacaaga      360

agggcgaggc ccgccgcucc cgccgcgccg ucacccuccc cucccacucc acccgcaagc      420

uccagacccg cucccagacc uggcucgagu cccgcgagua caccaagcac cucauccgcg      480

ucgagaacug gaucuuccgc aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc      540

uccucggcuc cuccaccucc cagaagguca ucuaccucgu caugauccuc cucaucgccc      600

ccgccuacuc cauccgcugc aucggcgucu ccaaccgcga cuucgucgag ggcauguccg      660

gcggcaccug ggucgacauc guccucgagc acggcggcug cgucaccguc auggcccagg      720

acaagcccac cgucgacauc gagcucguca ccaccaccgu cuccaacaug gccgaggucc      780

gcuccuacug cuacgaggcc uccaucuccg acauggccuc cgacucccgc ugccccaccc      840

agggcgaggc cuaccucgac aagcaguccg acacccagua cgucugcaag cgcacccucg      900

ucgaccgcgg cuggggcaac ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg      960

ccaaguucgc cugcuccaag aagaugaccg gcaaguccau ccagcccgag aaccucgagu     1020

accgcaucau gcucuccguc cacggcuccc agcacuccgg caugaucguc aacgacaccg     1080

gccacgagac cgacgagaac cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg     1140

aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc accggccucg     1200

acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc cacaaggagu     1260

gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag accgucgucg     1380

uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc gaggccgaga     1440

uggacggcgc caagggccgc cucuccuccg gccaccucaa gugccgccuc aagauggaca     1500

agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc uucaccaaga     1560

uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg     1620

gccccugcaa gguccccgcc cagauggccg ucgacaugca gacccucacc cccgucggcc     1680

gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag augaugcucg     1740

agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgag aagaagauca     1800

cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc gucggcggcg     1920

cccucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc aagucccucu     1980

ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucaug uggcucggcc     2040

ucaacaccaa gaacggcucc aucucccuca ugugccucgc ccucggcggc guccucaucu     2100

uccucuccac cgccgucucc gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  5874
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5874
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccuccgucgg caucgucggc cuccuccuca ccaccgccau ggccgccgag gucacccgcc      120

gcggcuccgc cuacuacaug uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc      180

ccaccacccu cggcaugaac aagugcuaca uccagaucau ggaccucggc cacaugugcg      240

acgccaccau guccuacgag ugccccaugc ucgacgaggg cgucgagccc gacgacgucg      300

acugcuggug caacaccacc uccaccuggg ucgucuacgg caccugccac cacaagaagg      360

gcgaggcccg ccgcucccgc cgcgccguca cccuccccuc ccacuccacc cgcaagcucc      420

agacccgcuc ccagaccugg cucgaguccc gcgaguacac caagcaccuc auccgcgucg      480

agaacuggau cuuccgcaac cccggcuucg cccucgccgc cgccgccauc gccuggcucc      540

ucggcuccuc caccucccag aaggucaucu accucgucau gauccuccuc aucgcccccg      600

ccuacuccau ccgcugcauc ggcgucucca accgcgacuu cgucgagggc auguccggcg      660

gcaccugggu cgacaucguc cucgagcacg gcggcugcgu caccgucaug gcccaggaca      720

agcccaccgu cgacaucgag cucgucacca ccaccgucuc caacauggcc gagguccgcu      780

ccuacugcua cgaggccucc aucuccgaca uggccuccga cucccgcugc cccacccagg      840

gcgaggccua ccucgacaag caguccgaca cccaguacgu cugcaagcgc acccucgucg      900

accgcggcug gggcaacggc ugcggccucu ucggcaaggg cucccucguc accugcgcca      960

aguucgccug cuccaagaag augaccggca aguccaucca gcccgagaac cucgaguacc     1020

gcaucaugcu cuccguccac ggcucccagc acuccggcau gaucgucaac gacaccggcc     1080

acgagaccga cgagaaccgc gccaaggucg agaucacccc caacuccccc cgcgccgagg     1140

ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc ggccucgacu     1200

ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac aaggaguggu     1260

uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc cacuggaaca     1320

acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc gucgucgucc     1380

ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag gccgagaugg     1440

acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag auggacaagc     1500

uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc accgacggcc     1620

ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc gucggccgcc     1680

ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug augcucgagc     1740

ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgagaag aagaucaccc     1800

accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc guccgcggcg     1860

ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc     1920

ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag ucccucuucg     1980

gcggcauguc cugguucucc cagauccuca ucggcacccu ccucaugugg cucggccuca     2040

acaccaagaa cggcuccauc ucccucaugu gccucgcccu cggcggcguc cucaucuucc     2100

ucuccaccgc cgucuccgcc ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  5875
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  5875
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5876
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5876
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5877
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5877
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cuccgucggc aucgucggcc uccuccucac caccgccaug gccgccgccg      120

ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc ggcgaggcca      180

ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug gaccucggcc      240

acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc gucgagcccg      300

acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc accugccacc      360

acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc cacuccaccc      420

gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc aagcaccuca      480

uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg      540

ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug auccuccuca      600

ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca      660

uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc accgucaugg      720

cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc aacauggccg      780

agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac ucccgcugcc      840

ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc ugcaagcgca      900

cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc ucccucguca      960

ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag cccgagaacc     1020

ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug aucgucaacg     1080

acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc aacucccccc     1140

gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag ccccgcaccg     1200

gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg cucguccaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag cgccagaccg     1380

ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc gcccucgagg     1440

ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc cgccucaaga     1500

uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag uacgccggca     1620

ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc cucacccccg     1680

ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac uccaagauga     1740

ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc ggcgagaaga     1800

agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg     1860

uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc ggcuccgucg     1920

gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc gccuucaagu     1980

cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc cucauguggc     2040

ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc     2100

ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  5878
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5878
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucuccuc ccgcaagcgc cgcucccacg acguccucac cguccaguuc cucauccucg      120

gcaugcuccu caugaccggc ggcgucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5879
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  5879
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucuccuc ccgcaagcgc cgcucccacg acguccucac cguccaguuc cucauccucg      120

gcaugcuccu caugaccggc gccgucaccc gccgcggcuc cgccuacuac auguaccucg      180

accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug aacaagugcu      240

acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac gagugcccca      300

ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc accuccaccu      360

gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc cgccgcgccg      420

ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc uggcucgagu      480

cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc aaccccggcu      540

ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc cagaagguca      600

ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc aucggcgucu      660

ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc guccucgagc      720

acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc gagcucguca      780

ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc uccaucuccg      840

acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac aagcaguccg      900

acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag aagaugaccg     1020

gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc cacggcuccc     1080

agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac cgcgccaagg     1140

ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg     1200

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga     1260

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1380

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1440

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuccuccg     1500

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1560

ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac ggcaccguca     1620

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc cagauggccg     1680

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1740

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1800

ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc ggcuccacca     1860

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1920

ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     2040

ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc aucucccuca     2100

ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  5880
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 2 opt

<400>  5880
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5881
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 2 opt

<400>  5881
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaccug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacgccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5882
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 2 opt

<400>  5882
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucggcu acgagaccga cgaggaccgc gccaaggucg     1140

aggucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc     1200

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca     1260

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg     1440

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uucuccggcc     1500

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1560

gcaccgccgc cuucaccuuc accaaggucc ccgccgagac ccuccacggc accgucaccg     1620

ucgaggucca guacgccggc accgacggcc ccugcaagau ccccguccag auggccgucg     1680

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1740

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1800

ucaucggcgu cggcgacaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1860

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1920

ccugggacuu cggcuccguc ggcggcgucu ucaacucccu cggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc cagauccuca     2040

ucggcacccu ccucgucugg cucggccuca acaccaagaa cggcuccauc ucccucaccu     2100

gccucgcccu cggcggcguc augaucuucc ucuccaccgc cgucuccgcc gacgucggcu     2160

gcuccgucga cuucuccaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5883
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 2 opt

<400>  5883
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5884
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 2 opt

<400>  5884
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg cuacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggcaagc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag auccccgucc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5885
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 2 opt

<400>  5885
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cacccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacgagaa ccgcgccaag gucgagguca     1140

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu     1200

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1380

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1440

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc ggccaccuca     1500

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1560

ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc accgucgagg     1620

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1680

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1740

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1800

gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1860

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1920

acuucggcuc cgucggcggc gucuucaacu cccucggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     2040

cccuccucgu cuggcucggc cucaacacca agaacggcuc caucucccuc accugccucg     2100

cccucggcgg cgucaugauc uuccucucca ccgccgucuc cgccgacguc ggcugcuccg     2160

ucgacuucuc caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5886
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 2 opt

<400>  5886
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accgcaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg cuacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggcaagc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag auccccgucc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5887
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 2 opt

<400>  5887
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaccguca acgacaucgg cuacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggcaagc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag auccccgucc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5888
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 2 opt

<400>  5888
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucggcu acgagaccga cgagaaccgc gccaaggucg     1140

aggucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc     1200

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca     1260

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1380

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg     1440

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggcaagcuc uucuccggcc     1500

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1560

gcaccgccgc cuucaccuuc accaaggucc ccgccgagac ccuccacggc accgucaccg     1620

ucgaggucca guacgccggc accgacggcc ccugcaagau ccccguccag auggccgucg     1680

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1740

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1800

ucaucggcgu cggcgacaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1860

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1920

ccugggacuu cggcuccguc ggcggcgucu ucaacucccu cggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc cagauccuca     2040

ucggcacccu ccucgucugg cucggccuca acaccaagaa cggcuccauc ucccucaccu     2100

gccucgcccu cggcggcguc augaucuucc ucuccaccgc cgucuccgcc gacgucggcu     2160

gcuccgucga cuucuccaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  5889
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 2 opt

<400>  5889
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggccgcg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5890
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 2 opt

<400>  5890
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga cgccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5891
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 2 opt

<400>  5891
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggccu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5892
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 2 opt

<400>  5892
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg uaccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5893
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 2 opt

<400>  5893
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagauccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5894
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 2 opt

<400>  5894
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccucgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5895
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 2 opt

<400>  5895
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccac ccucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5896
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 2 opt

<400>  5896
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gucgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5897
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 2 opt

<400>  5897
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccgccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ccccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5898
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 2 opt

<400>  5898
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccucca      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5899
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 2 opt

<400>  5899
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccacccgcc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5900
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 2 opt

<400>  5900
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcuccgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacacccag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5901
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 2 opt

<400>  5901
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccaacguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgcccgccgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5902
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 2 opt

<400>  5902
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga gaccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5903
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 2 opt

<400>  5903
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc cuccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5904
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 2 opt

<400>  5904
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccaacguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg auguuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5905
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 2 opt

<400>  5905
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg auccuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc aucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5906
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 2 opt

<400>  5906
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucuc caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggcca      840

ccaucuccga caucgccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgccgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5907
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 2 opt

<400>  5907
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcacc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5908
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 2 opt

<400>  5908
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagcuc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5909
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 2 opt

<400>  5909
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga gaccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcau cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5910
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 2 opt

<400>  5910
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaucgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5911
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 2 opt

<400>  5911
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcguc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucuccuucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5912
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 2 opt

<400>  5912
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggcaagc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5913
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 2 opt

<400>  5913
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga ggcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5914
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 2 opt

<400>  5914
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcgccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5915
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 2 opt

<400>  5915
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc cggcaaggag aagaagcgcc gcggcgccga caccaacguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgcccgccgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5916
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 2 opt

<400>  5916
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga gaccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaaggac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5917
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 2 opt

<400>  5917
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggucucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5918
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 2 opt

<400>  5918
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccaacguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacauga      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5919
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 2 opt

<400>  5919
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccgcccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagacccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5920
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 2 opt

<400>  5920
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga gaccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccu cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5921
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 2 opt

<400>  5921
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcaucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcauccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5922
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 2 opt

<400>  5922
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaccug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca uccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacgccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5923
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 2 opt

<400>  5923
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cagcgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5924
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 2 opt

<400>  5924
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccaggcccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5925
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 2 opt

<400>  5925
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5926
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 2 opt

<400>  5926
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcaccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacac cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga gggcaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5927
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 2 opt

<400>  5927
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccgcca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augcucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgccc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgcc accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag accgucgacg     1620

gcaccgucac cgucgagggc caguacggcg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucaucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

gcggcgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5928
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 2 opt

<400>  5928
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcaccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucuccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgaggagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5929
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 2 opt

<400>  5929
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcaccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5930
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 2 opt

<400>  5930
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcaccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgucg aggucacccg ccgcggcaac gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc augggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5931
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 2 opt

<400>  5931
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcaccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     2100

ucucccucgu cugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5932
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 2 opt

<400>  5932
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcaccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgucg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccgcc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5933
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 2 opt

<400>  5933
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgucg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc cucggcauga      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccguca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5934
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 2 opt

<400>  5934
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgucg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcgagg ccaucuccuu ccccaccacc cucggcguca      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg uaccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5935
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 2 opt

<400>  5935
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgucg aggucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu ccccaccacc cucggcguca      240

acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5936
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 2 opt

<400>  5936
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cacccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5937
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 2 opt

<400>  5937
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccaac cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcguccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5938
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 2 opt

<400>  5938
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5939
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 2 opt

<400>  5939
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccaac cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucgcc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacgagaa ccgcgccaag gucgagguca     1140

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu     1200

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1380

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1440

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc ggccaccuca     1500

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1560

ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc accgucgagg     1620

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1680

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1740

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1800

gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1860

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1920

acuucggcuc cgucggcggc gucuucaacu cccucggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     2040

cccuccucgu cuggcucggc cucaacacca agaacggcuc caucucccuc accugccucg     2100

cccucggcgg cgucaugauc uuccucucca ccgccgucuc cgccgacguc ggcugcuccg     2160

ucgacuucuc caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5940
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 2 opt

<400>  5940
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5941
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 2 opt

<400>  5941
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc agcacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gucugcaccg ccgccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5942
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 2 opt

<400>  5942
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga cgccuccauc ggcaucgucu      120

cccuccuccu caccaccguc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgucaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacgagaa ccgcgccaag gucgagguca     1140

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu     1200

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac uccggcgccg     1320

acaccgagac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1380

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1440

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca     1500

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1560

ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc accgucgagg     1620

uccaguacgc cggccgcgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1680

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1740

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1800

gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccauc aucggcaagg     1860

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1920

acuucggcuc cgucggcggc gucuucaacu cccucggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     2040

cccuccucgu cuggcucggc cucaacacca agaacggcuc caucucccuc accugccucg     2100

cccucggcgg cgucaugauc uuccucucca ccgccgucuc cgccgacguc ggcugcuccg     2160

ucgacuucuc caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5943
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 2 opt

<400>  5943
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucgucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      540

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      600

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacgagaa ccgcgccaag gucgagguca     1140

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu     1200

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc     1260

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1380

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1440

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc ggccaccuca     1500

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1560

ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc accgucgagg     1620

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1680

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1740

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1800

gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1860

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1920

acuucggcuc cgucggcggc gucuucaacu cccucggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     2040

cccuccucgu cuggcucggc cucaacacca agaacggcuc caucucccuc accugccucg     2100

cccucggcgg cgucaugauc uuccucucca ccgccgucuc cgccgacguc ggcugcuccg     2160

ucgacuucuc caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  5944
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 2 opt

<400>  5944
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc cucggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgagacc cgccgcuccc      420

gccgcuccgu cucccuccgc uaccacuaca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac aagaagcacc ucaucauggu cgagaacugg aucuuccgca      540

accccggcuu cgccaucguc uccgucgcca ucaccuggcu caugggcucc cucaccuccc      600

agaaggucau cuaccucguc augaucgucc ucaucguccc cgccuacucc aucuccugca      660

ucggcgucuc caaccgcgac cucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccgaga uggcccagga caagcccacc gucgacaucg      780

agcucgucac caugaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

cccucuccga cauggccucc gccucccgcu gccccaccca gggcgagccc ucccucgaca      900

agcaguccga cacccagucc gucugcaagc gcacccucgg cgaccgcggc uggggcaacg      960

gcugcggcau cuucggcaag ggcucccucg ucaccugcuc caaguucacc ugcugcaaga     1020

agaugcccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cuccccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguccgccg gcaccgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1740

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  5945
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 2 opt

<400>  5945
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaggag cgcaagcgcc gcggcgccga caccuccauc ggcaucaucg      120

gccuccuccu caccaccgcc auggccgccg agaucacccg ccgcggcucc gccuacuaca      180

uguaccucga ccgcuccgac gccggcaagg ccaucuccuu cgccaccacc augggcguca      240

acaagugcca cguccagauc auggaccucg gccacaugug cgacgccacc auguccuacg      300

agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc cgccccuccc      420

gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      480

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg auccuccgca      540

accccggcuu caccgucgcc gcccucgcca uggccuggcu ccucggcaac uccaccuccc      600

agcgcgucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      660

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      720

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      780

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      840

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      900

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      960

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga     1020

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc     1080

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc     1140

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg     1200

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1380

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1440

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1500

ucuucuccgg ccaccucaag ugccgcauca agauggacaa gcuccgccuc aagggcgucu     1560

ccuacucccu cugcaccgcc gccuucaccu ucuccaaggu ccccgccgag acccuccacg     1620

gcaccgucac cgucgagguc caguacgccg gccgcgacgg ccccugcaag guccccgccc     1680

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaacccca     1740

ucaucaccga guccaccgag aacucccgca ugaugcucga gcucgacccc cccuucggcg     1800

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1860

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1920

ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc cucggcaagg     1980

gcguccacca ggucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     2040

cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag aacggcucca     2100

ucucccucgc cugccucgcc cucggcggcg ccaugaucuu ccucuccacc gccgucuccg     2160

ccgacgucgg cugcuccguc gacuucugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  5946
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 2 opt

<400>  5946
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5947
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 2 opt

<400>  5947
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac accugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaacg     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5948
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 2 opt

<400>  5948
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cggcuacgag accgacgagg     1080

accgcgccaa ggucgagguc acccccaacu ccccccgcgc cgaggccacc cucggcggcu     1140

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu     1200

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1260

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1320

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1380

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1440

gccucuucuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1500

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gguccccgcc gagacccucc     1560

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagauccccg     1620

uccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1680

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1740

gcgacuccua caucgucauc ggcgucggcg acaagaagau cacccaccac uggcaccgcu     1800

ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag cgcauggccg     1860

uccucggcga caccgccugg gacuucggcu ccgucggcgg cgucuucaac ucccucggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc auguccuggu     1980

ucucccagau ccucaucggc acccuccucg ucuggcucgg ccucaacacc aagaacggcu     2040

ccaucucccu caccugccuc gcccucggcg gcgucaugau cuuccucucc accgccgucu     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5949
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 2 opt

<400>  5949
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5950
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 2 opt

<400>  5950
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggcuacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg caagcucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5951
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 2 opt

<400>  5951
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccgc aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggcuacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg caagcucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5952
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 2 opt

<400>  5952
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugac cgucaacgac aucggcuacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg caagcucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5953
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 2 opt

<400>  5953
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau cgucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucaccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac gagaaccgcg     1080

ccaaggucga ggucaccccc aacucccccc gcgccgaggc cacccucggc ggcuucggcu     1140

cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca     1200

ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc ccccuccccu     1260

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cucgucgagu     1320

ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag gagggcgccg     1380

uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag ggccgccucu     1440

ucuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag ggcgucuccu     1500

acucccucug caccgccgcc uucaccuuca ccaagguccc cgccgagacc cuccacggca     1560

ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc cccgcccaga     1620

uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc aaccccguca     1680

ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc uucggcgacu     1740

ccuacaucgu caucggcguc ggcgacaaga agaucaccca ccacuggcac cgcuccggcu     1800

ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug gccguccucg     1860

gcgacaccgc cugggacuuc ggcuccgucg gcggcgucuu caacucccuc ggcaagggca     1920

uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc ugguucuccc     1980

agauccucau cggcacccuc cucgucuggc ucggccucaa caccaagaac ggcuccaucu     2040

cccucaccug ccucgcccuc ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  5954
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 2 opt

<400>  5954
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cggcuacgag accgacgaga     1080

accgcgccaa ggucgagguc acccccaacu ccccccgcgc cgaggccacc cucggcggcu     1140

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu     1200

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1260

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1320

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1380

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggca     1440

agcucuucuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1500

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gguccccgcc gagacccucc     1560

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagauccccg     1620

uccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1680

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1740

gcgacuccua caucgucauc ggcgucggcg acaagaagau cacccaccac uggcaccgcu     1800

ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag cgcauggccg     1860

uccucggcga caccgccugg gacuucggcu ccgucggcgg cgucuucaac ucccucggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc auguccuggu     1980

ucucccagau ccucaucggc acccuccucg ucuggcucgg ccucaacacc aagaacggcu     2040

ccaucucccu caccugccuc gcccucggcg gcgucaugau cuuccucucc accgccgucu     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  5955
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 2 opt

<400>  5955
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg ccgcggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5956
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 2 opt

<400>  5956
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggccucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5957
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 2 opt

<400>  5957
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acugguaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5958
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 2 opt

<400>  5958
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca cccagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5959
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 2 opt

<400>  5959
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag auccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5960
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 2 opt

<400>  5960
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccu cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5961
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 2 opt

<400>  5961
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccacccucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5962
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 2 opt

<400>  5962
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgucgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5963
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 2 opt

<400>  5963
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc gccugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgccccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5964
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 2 opt

<400>  5964
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cuccaagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5965
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 2 opt

<400>  5965
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac ccgccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5966
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 2 opt

<400>  5966
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcacccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5967
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 2 opt

<400>  5967
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acucccuccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5968
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 2 opt

<400>  5968
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcaucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5969
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 2 opt

<400>  5969
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggauccu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcaucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5970
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 2 opt

<400>  5970
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucuccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccaccauc uccgacaucg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccgcc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5971
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 2 opt

<400>  5971
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agcuccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5972
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 2 opt

<400>  5972
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ucgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5973
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 2 opt

<400>  5973
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgucaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc uucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5974
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 2 opt

<400>  5974
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg caagcucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5975
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 2 opt

<400>  5975
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca aggacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5976
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 2 opt

<400>  5976
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggaggccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5977
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 2 opt

<400>  5977
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggccg     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag acccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5978
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 2 opt

<400>  5978
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcgcc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5979
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 2 opt

<400>  5979
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccucgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5980
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 2 opt

<400>  5980
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ucuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5981
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 2 opt

<400>  5981
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc aucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcauc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5982
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 2 opt

<400>  5982
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac accugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccauccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaacg     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5983
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 2 opt

<400>  5983
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccagcg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5984
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 2 opt

<400>  5984
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccagg      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5985
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 2 opt

<400>  5985
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucucc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgagg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5986
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 2 opt

<400>  5986
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacaccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagggca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcguccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5987
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 2 opt

<400>  5987
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5988
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 2 opt

<400>  5988
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgucgagguc acccgccgcg      120

gcaacgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccaccauggg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5989
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 2 opt

<400>  5989
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucgucugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5990
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 2 opt

<400>  5990
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucaacaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucgucugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5991
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 2 opt

<400>  5991
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgucgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccgccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5992
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 2 opt

<400>  5992
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgucgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgucaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5993
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 2 opt

<400>  5993
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgucgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg cgaggccauc uccuucccca      180

ccacccucgg cgucaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acugguaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5994
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 2 opt

<400>  5994
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgucgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucccca      180

ccacccucgg cgucaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac aucggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5995
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 2 opt

<400>  5995
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau cgucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucaccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5996
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 2 opt

<400>  5996
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccaaccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac aucggccacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcguc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5997
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 2 opt

<400>  5997
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac aucggccacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  5998
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 2 opt

<400>  5998
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccaaccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac gagaaccgcg     1080

ccaaggucga ggucaccccc aacucccccc gcgccgaggc cacccucggc ggcuucggcu     1140

cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca     1200

ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc ccccuccccu     1260

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cucgucgagu     1320

ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag gagggcgccg     1380

uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag ggccgccucu     1440

ucuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag ggcgucuccu     1500

acucccucug caccgccgcc uucaccuuca ccaagguccc cgccgagacc cuccacggca     1560

ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc cccgcccaga     1620

uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc aaccccguca     1680

ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc uucggcgacu     1740

ccuacaucgu caucggcguc ggcgacaaga agaucaccca ccacuggcac cgcuccggcu     1800

ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug gccguccucg     1860

gcgacaccgc cugggacuuc ggcuccgucg gcggcgucuu caacucccuc ggcaagggca     1920

uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc ugguucuccc     1980

agauccucau cggcacccuc cucgucuggc ucggccucaa caccaagaac ggcuccaucu     2040

cccucaccug ccucgcccuc ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  5999
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 2 opt

<400>  5999
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau cgucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6000
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 2 opt

<400>  6000
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau cgucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccagcac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac aucggccacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgucug caccgccgcc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6001
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 2 opt

<400>  6001
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau cgucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac gagaaccgcg     1080

ccaaggucga ggucaccccc aacucccccc gcgccgaggc cacccucggc ggcuucggcu     1140

cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca     1200

ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc ccccuccccu     1260

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cucgucgagu     1320

ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag gagggcgccg     1380

uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag ggccgccucu     1440

ucuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag ggcgucuccu     1500

acucccucug caccgccgcc uucaccuuca ccaagguccc cgccgagacc cuccacggca     1560

ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc cccgcccaga     1620

uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc aaccccguca     1680

ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc uucggcgacu     1740

ccuacaucgu caucggcguc ggcgacaaga agaucaccca ccacuggcac cgcuccggcu     1800

ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug gccguccucg     1860

gcgacaccgc cugggacuuc ggcuccgucg gcggcgucuu caacucccuc ggcaagggca     1920

uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc ugguucuccc     1980

agauccucau cggcacccuc cucgucuggc ucggccucaa caccaagaac ggcuccaucu     2040

cccucaccug ccucgcccuc ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6002
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 2 opt

<400>  6002
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgagauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

agacccgccg cucccgccgc uccgucuccc uccgcuacca cuacacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaagaa gcaccucauc auggucgaga      480

acuggaucuu ccgcaacccc ggcuucgcca ucgucuccgu cgccaucacc uggcucaugg      540

gcucccucac cucccagaag gucaucuacc ucgucaugau cguccucauc guccccgccu      600

acuccaucuc cugcaucggc gucuccaacc gcgaccucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgagauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccauga ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccucccuc uccgacaugg ccuccgccuc ccgcugcccc acccagggcg      840

agcccucccu cgacaagcag uccgacaccc aguccgucug caagcgcacc cucggcgacc      900

gcggcugggg caacggcugc ggcaucuucg gcaagggcuc ccucgucacc ugcuccaagu      960

ucaccugcug caagaagaug cccggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcuccc cguccacggc ucccagcacu ccggcaugau cgucaacgac aucggccacg     1080

agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagguccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccaguc cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgucuuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6003
<211>  2446
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 2 opt

<400>  6003
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccg       60

ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc cgccgcggcu      120

ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc uuccccacca      180

cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug ugcgacgcca      240

ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac gucgacugcu      300

ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag aagggcgagg      360

cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag cuccagaccc      420

gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc gucgagaacu      480

ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg cuccucggcu      540

ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc cccgccuacu      600

ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc ggcggcaccu      660

gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag gacaagccca      720

ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc cgcuccuacu      780

gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc cagggcgagg      840

ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc gucgaccgcg      900

gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc gccaaguucg      960

ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag uaccgcauca     1020

ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc ggccacgaga     1080

ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc gaggccaccc     1140

ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc gacuucuccg     1200

accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag ugguuccacg     1260

acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg aacaacaagg     1320

aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc guccucggcu     1380

cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag auggacggcg     1440

ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac aagcuccgcc     1500

ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag auccccgccg     1560

agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac ggccccugca     1620

agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc cgccucauca     1680

ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc gagcucgacc     1740

cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc acccaccacu     1800

ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc ggcgccaagc     1860

gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc gcccucaacu     1920

cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc uucggcggca     1980

uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc cucaacacca     2040

agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc uuccucucca     2100

ccgccgucuc cgccugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2160

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2220

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2280

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2400

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2446


<210>  6004
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 2 opt

<400>  6004
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgccc     1860

gccgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6005
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 2 opt

<400>  6005
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggauguu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6006
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 2 opt

<400>  6006
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caugaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6007
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 2 opt

<400>  6007
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgagguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgccauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugcu cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cgcccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgccaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagaccgu cgacggcacc gucaccgucg agggccagua cggcggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagauca ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccggcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6008
<211>  2440
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 2 opt

<400>  6008
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacgccuc caucggcauc gucucccucc uccucaccac cgucauggcc gccgagauca      120

cccgccgcgg cuccgccuac uacauguacc ucgaccgcuc cgacgccggc aaggccaucu      180

ccuucgucac cacccucggc gucaacaagu gccacgucca gaucauggac cucggccaca      240

ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc gagcccgacg      300

acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc ugccaccaca      360

agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac uccacccgca      420

agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag caccucauca      480

aggucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu      540

ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc cuccucaucg      600

cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu      660

ccggcggcac cugggucgac gucguccucg agcacggcgg cugcgucacc gucauggccc      720

aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac auggccgagg      780

uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc cgcugcccca      840

cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc aagcgcaccc      900

ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc cucgucaccu      960

gcgccaaguu caccugcucc aagaagauga ccggcaaguc cauccagccc gagaaccucg     1020

aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc gucaacgacg     1080

agaaccgcgc caaggucgag gucaccccca acuccccccg cgccgaggcc acccucggcg     1140

gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu     1200

acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc cacgacaucc     1260

cccuccccug gcacuccggc gccgacaccg agacccccca cuggaacaac aaggaggccc     1320

ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc ggcucccagg     1380

agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac ggcgccaagg     1440

gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc cgccucaagg     1500

gcgucuccua cucccucugc accgccgccu ucaccuucac caaggucccc gccgagaccc     1560

uccacggcac cgucaccguc gagguccagu acgccggccg cgacggcccc ugcaaggucc     1620

ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc aucaccgcca     1680

accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc gacccccccu     1740

ucggcgacuc cuacaucguc aucggcgucg gcgacaagaa gaucacccac cacuggcacc     1800

gcuccggcuc caucaucggc aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg     1860

ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgucuuc aacucccucg     1920

gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc ggcauguccu     1980

gguucuccca gauccucauc ggcacccucc ucgucuggcu cggccucaac accaagaacg     2040

gcuccaucuc ccucaccugc cucgcccucg gcggcgucau gaucuuccuc uccaccgccg     2100

ucuccgccug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2160

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2220

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2280

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2400

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2440


<210>  6009
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 2 opt

<400>  6009
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccuc caucggcauc aucggccucc uccucaccac cgccauggcc gccgagauca      120

cccgccgcgg cuccgccuac uacauguacc ucgaccgcuc cgacgccggc aaggccaucu      180

ccuucgccac caccaugggc gucaacaagu gccacgucca gaucauggac cucggccaca      240

ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc gagcccgacg      300

acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc ugccaccaca      360

agaagggcga ggcccgcccc ucccgccgcg ccgucacccu ccccucccac uccacccgca      420

agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag caccucauca      480

aggucgagaa cuggauccuc cgcaaccccg gcuucaccgu cgccgcccuc gccauggccu      540

ggcuccucgg caacuccacc ucccagcgcg ucaucuaccu cgucaugauc cuccucaucg      600

cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu      660

ccggcggcac cugggucgac gucguccucg agcacggcgg cugcgucacc gucauggccc      720

aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac auggccgagg      780

uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc cgcugcccca      840

cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc aagcgcaccc      900

ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc cucgucaccu      960

gcgccaaguu caccugcucc aagaagauga ccggcaaguc cauccagccc gagaaccucg     1020

aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc gucaacgaca     1080

ucggccacga gaccgacgag aaccgcgcca aggucgaggu cacccccaac uccccccgcg     1140

ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc cgcaccggcc     1200

ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc guccacaagg     1260

agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc cagaccgucg     1380

ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg     1440

agauggacgg cgccaagggc cgccucuucu ccggccaccu caagugccgc aucaagaugg     1500

acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc accuucucca     1560

agguccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac gccggccgcg     1620

acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc acccccgucg     1680

gccgccucau caccgccaac cccaucauca ccgaguccac cgagaacucc cgcaugaugc     1740

ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc gacaagaaga     1800

ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc uccgucggcg     1920

gcgucuucaa cucccucggc aagggcgucc accaggucuu cggcgccgcc uucaaguccc     1980

ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc gucuggcucg     2040

gccucaacac caagaacggc uccaucuccc ucgccugccu cgcccucggc ggcgccauga     2100

ucuuccucuc caccgccguc uccgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6010
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 2 opt

<400>  6010
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6011
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 2 opt

<400>  6011
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6012
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 2 opt

<400>  6012
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucggcu acgagaccga cgaggaccgc gccaaggucg      600

aggucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc      660

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca      720

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg      780

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg      840

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg      900

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uucuccggcc      960

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1020

gcaccgccgc cuucaccuuc accaaggucc ccgccgagac ccuccacggc accgucaccg     1080

ucgaggucca guacgccggc accgacggcc ccugcaagau ccccguccag auggccgucg     1140

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1200

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1260

ucaucggcgu cggcgacaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1320

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1380

ccugggacuu cggcuccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6013
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 2 opt

<400>  6013
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6014
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 2 opt

<400>  6014
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg cuacgagacc gacgagaacc      600

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggcaagc      960

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag auccccgucc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6015
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 2 opt

<400>  6015
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaccguca acgacaucgg cuacgagacc gacgagaacc      600

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggcaagc      960

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag auccccgucc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6016
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 2 opt

<400>  6016
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacgagaa ccgcgccaag gucgagguca      600

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu      660

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc      720

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg      780

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg      840

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg      900

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc ggccaccuca      960

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1020

ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc accgucgagg     1080

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1140

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1200

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1260

gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1320

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1380

acuucggcuc cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  6017
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 2 opt

<400>  6017
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucggcu acgagaccga cgagaaccgc gccaaggucg      600

aggucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc      660

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca      720

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg      780

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg      840

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg      900

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggcaagcuc uucuccggcc      960

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1020

gcaccgccgc cuucaccuuc accaaggucc ccgccgagac ccuccacggc accgucaccg     1080

ucgaggucca guacgccggc accgacggcc ccugcaagau ccccguccag auggccgucg     1140

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1200

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1260

ucaucggcgu cggcgacaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1320

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1380

ccugggacuu cggcuccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6018
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 2 opt

<400>  6018
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc cuccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6019
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 2 opt

<400>  6019
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucca       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6020
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 2 opt

<400>  6020
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg uaccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6021
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 2 opt

<400>  6021
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggcaagc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6022
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 2 opt

<400>  6022
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacacccag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6023
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 2 opt

<400>  6023
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagauccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6024
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 2 opt

<400>  6024
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgcccgccgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6025
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 2 opt

<400>  6025
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggucucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6026
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 2 opt

<400>  6026
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaucgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6027
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 2 opt

<400>  6027
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc aucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6028
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 2 opt

<400>  6028
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccu cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6029
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 2 opt

<400>  6029
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcacc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6030
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 2 opt

<400>  6030
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccucgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6031
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 2 opt

<400>  6031
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga ggcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6032
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 2 opt

<400>  6032
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccgcccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6033
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 2 opt

<400>  6033
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucuc caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggcca      300

ccaucuccga caucgccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgccgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6034
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 2 opt

<400>  6034
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucuccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgaggagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6035
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 2 opt

<400>  6035
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagcuc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6036
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 2 opt

<400>  6036
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcaucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcauccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6037
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 2 opt

<400>  6037
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcguc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6038
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 2 opt

<400>  6038
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcgccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6039
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 2 opt

<400>  6039
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacac cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga gggcaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6040
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 2 opt

<400>  6040
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccgcca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augcucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgccc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgcc accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag accgucgacg     1080

gcaccgucac cgucgagggc caguacggcg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6041
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 2 opt

<400>  6041
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccgcc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6042
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 2 opt

<400>  6042
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucaa caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6043
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 2 opt

<400>  6043
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcgccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6044
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 2 opt

<400>  6044
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6045
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 2 opt

<400>  6045
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg uaccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6046
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 2 opt

<400>  6046
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6047
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 2 opt

<400>  6047
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc      600

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6048
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 2 opt

<400>  6048
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc      600

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gucugcaccg ccgccaaggu ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6049
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 2 opt

<400>  6049
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacgagaa ccgcgccaag gucgagguca      600

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu      660

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc      720

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac uccggcgccg      780

acaccgagac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg      840

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg      900

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca      960

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1020

ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc accgucgagg     1080

uccaguacgc cggccgcgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1140

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1200

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1260

gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccauc aucggcaagg     1320

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1380

acuucggcuc cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  6050
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 2 opt

<400>  6050
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaggucau cuaccucguc augaucgucc ucaucguccc cgccuacucc aucuccugca      120

ucggcgucuc caaccgcgac cucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccgaga uggcccagga caagcccacc gucgacaucg      240

agcucgucac caugaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

cccucuccga cauggccucc gccucccgcu gccccaccca gggcgagccc ucccucgaca      360

agcaguccga cacccagucc gucugcaagc gcacccucgg cgaccgcggc uggggcaacg      420

gcugcggcau cuucggcaag ggcucccucg ucaccugcuc caaguucacc ugcugcaaga      480

agaugcccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cuccccgucc      540

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc      600

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguccgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6051
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 2 opt

<400>  6051
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      600

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1200

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6052
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 2 opt

<400>  6052
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agcgcgucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      120

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      180

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      240

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      300

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      360

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      420

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      480

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      540

acggcuccca gcacuccggc augaucguca acgacaucgg ccacgagacc gacgagaacc      600

gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      660

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg      840

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg      900

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc      960

ucuucuccgg ccaccucaag ugccgcauca agauggacaa gcuccgccuc aagggcgucu     1020

ccuacucccu cugcaccgcc gccuucaccu ucuccaaggu ccccgccgag acccuccacg     1080

gcaccgucac cgucgagguc caguacgccg gccgcgacgg ccccugcaag guccccgccc     1140

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaacccca     1200

ucaucaccga guccaccgag aacucccgca ugaugcucga gcucgacccc cccuucggcg     1260

acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg caccgcuccg     1320

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1380

ucggcgacac cgccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6053
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  6053
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacauag      720

uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca guggacauug      780

agcuggucac cacgacuguu ucaaauaugg cugaagugcg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu ggaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuugugg     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguaguugu gcugggcagc caggaggggg     1440

ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggaa acccuccaug     1620

gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag acccugacuc cugucggcag guuaauuaca gccaacccag     1740

ugaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggacac agccugggau uucggaagug uaggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag ugcugauuuu ccugucaacg gccguuagug     2160

cugacgucgg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6054
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 opt

<400>  6054
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacacuug ugacgccacc augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacaa      360

ccaguacuug gguggucuau gguacauguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacccuaccg ucccauucua cgaggaagcu ccaaacucgg ucgcagaccu      480

ggcuggaguc acgcgaauac acaaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacuuccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguaccugg guugacgugg      720

ucuuagagca uggcgggugu gugacaguga uggcacagga uaagccaacc gucgacauag      780

agcugguuac gacuacagug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccaccca aggagaagcc uaccuugaca      900

aacagagcga uacucaguau gugugcaaga gaacauuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugaccugcgc aaaguuugcu ugcucuaaga     1020

aaaugacggg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauaccgg ccaugagaca gacgagaauc     1140

gagccaaagu cgaaaucacu ccaaacucac cccgcgcuga ggccacccuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa cagggcucga cuuuagugau cuguauuacu     1260

uaacuaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau accggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac ugcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacggcc gcauucacau uuaccaaaau accugcggaa acucuccaug     1620

gcaccgugac aguugaggug caguacgcug guaccgacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acucugacac cugugggcag guuaaucacg gccaacccag     1740

uuauuaccga aaguacugaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac acaccacugg caucguucug     1860

gaucaaccau cgguaaggcu uuugaggcca cagugcgggg cgcaaaacga auggcuguac     1920

ucggggacac ugccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacc cuccugaugu ggcuuggccu gaaugcaaag aacgguagca     2100

uauccuugau gugucucgcc uuagggggag uucugaucuu ccugucaacg gcugucagug     2160

ccgacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6055
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opt

<400>  6055
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca uuggggguga      240

auaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu cacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuauaaaagu ggaaaauugg aucuuuagaa      540

acccgggauu cgcucugguu gccguggcga uugcaugguu gcucggcagu uccacaucuc      600

agaaggucau cuaccuggug augauucugc ucaucgcccc cgcuuauagc auaaggugca      660

uuggcguauc caaucgggau uuuguugagg ggaugucagg agguacuugg guggacgucg      720

uguuagaaca cggcgggugu gugaccguca uggcccagga uaaaccuaca guugacaucg      780

agcuggugac cacgacugua aguaacaugg cagaggugcg uagcuacugc uacgaggcuu      840

cuauuuccga uauggccuca gacagccgcu guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gucugcaaaa gaacuuuggu ggacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag uuacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauc cagcccgaaa accucgagua ucggaucaug cugucggugc     1080

acggaucuca acauuccggu augauuggcu acgaaaccga ugaggaccga gcaaaggucg     1140

aggugacacc uaauucacca cgcgcugaag ccacucuugg gggauucggc agucuggguu     1200

uggauuguga gcccagaacc gggcucgacu uuagcgaucu guauuacuua acaaugaaca     1260

auaaacacug gcugguacau aaggaauggu uccacgacau accucuccca uggcacgcug     1320

gcgccgauac uggaaccccg cauuggaaca auaaagaggc acuuguugaa uuuaaggacg     1380

cccacgcuaa gaggcagaca guggucgugc ugggcuccca ggagggggcg guccauaccg     1440

cauuggccgg agcucuagag gccgaaaugg auggugcaaa aggccggcug uucucugggc     1500

accucaagug ccgccugaaa auggacaaac uuagacugaa gggagugagc uauucauugu     1560

guacugccgc uuuuacguuc acaaagguuc ccgccgagac ccuccauggc acugugaccg     1620

uagaagucca guacgcaggu acagacgggc cuugcaagau cccagugcaa auggcgguug     1680

auaugcagac ccugacuccc gugggcaggu uaauuacagc uaacccuguc aucacggaga     1740

guaccgaaaa uuccaaaaug augcuggagc uagacccacc cuuuggagau agcuacauag     1800

ugauuggcgu aggggacaag aaaaucacuc accacuggca ucguucugga ucaacaaucg     1860

guaaggccuu cgaggcaacc gugcggggcg ccaaacgaau ggcuguucuc ggggauacag     1920

ccugggacuu cggaaguguc ggcggugugu uuaacagccu ggggaagggc auucaccaga     1980

ucuucggagc agcuuuuaag ucccuuuucg gcgggauguc uugguuuucg caaauuuuga     2040

uaggaacucu gcucgugugg cugggccuua acaccaaaaa ugguagcauc ucccugacgu     2100

guuuggcccu cgggggaguc augauuuucc ugucaaccgc gguuagugcc gaugugggcu     2160

gcucugugga cuuuagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6056
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 2 opt

<400>  6056
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6057
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  6057
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6058
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 2 opt

<400>  6058
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac acuugugaug      240

ccaccauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa cacaaccagu acuugggugg ucuauggcac augucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugaccc uaccguccca uucuacgagg aaacuccaaa      420

cucggucgca gaccuggcug gagucacgcg aguacacaaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac uucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

ccuggguuga uguggucuua gaacauggug gcugugugac agugauggcu caggacaagc      720

caaccgucga uauugagcug guuacgacua cagugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acccaagggg      840

aggccuaccu ugacaagcag agcgacacuc aguaugugug caaaaggaca uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugacc ugcgcgaaau      960

uugccugcuc uaagaaaaug acgggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac accggucaug     1080

aaacagauga gaaccgcgca aaggucgaga uuacuccaaa uucccccaga gcugaagcca     1140

cccuuggcgg guucggauca cugggcuugg acugugagcc uaggacaggu cucgauuuua     1200

gugaccugua uuacuuaacu augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacaccgg cacaccccau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagaccguu gugguccugg     1380

gaagccagga gggcgcggug cauacugcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cggccgcuuu cacauuuacc aagaucccug     1560

ccgagacucu ccauggaacc gugacaguug aagugcagua cgcaggcacc gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacucu gacaccugug gggagguuaa     1680

uuacggccaa cccaguuaua accgagagua cugaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacacacc     1800

acuggcaucg gucuggguca accauaggaa aggcauuuga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauacugcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacccuccu gauguggcuu ggacugaacg     2040

ccaagaaugg cagcaucucc uugauguguc ucgcucuggg ugggguuuua auuuuccugu     2100

caacggcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6059
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 2 opt

<400>  6059
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauu aagguggaga      480

acuggaucuu uagaaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauuag gugcaucggc guauccaacc gggacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcacggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgcagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaagaacu uugguggaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauccagcc cgaaaaucuc gaauaucgga     1020

uuaugcuguc ggugcacggg ucucaacauu ccggaaugau cgguuacgag accgacgaag     1080

aucgugcgaa ggucgaggug acaccuaacu caccacgcgc cgaggcaacu cuuggcgggu     1140

ucggaagucu gggcuuggac ugugaaccca gaaccggucu cgauuuuagc gaccuguauu     1200

acuuaacaau gaauaacaaa cacuggcugg uacauaagga gugguuccac gauaucccuc     1260

ucccauggca cgcuggggcc gacacuggca ccccgcauug gaauaacaaa gaagcucuug     1320

uugaguuuaa ggaugcccac gcaaaaaggc agacaguggu cgugcuggga ucccaggaag     1380

gcgccgucca uaccgcuuug gcgggggcac uagaggccga gauggacgga gcuaaggguc     1440

ggcuguucuc uggccaccuc aagugccgac ugaaaaugga uaaacuucgc cugaaggggg     1500

ugagcuauuc auuguguacu gccgcauuua cguucacaaa gguucccgcc gaaacccucc     1560

auggaacugu gaccguagag guccaguacg cuggcacaga cgguccuugc aaaauuccag     1620

ugcaaauggc cguugacaug cagacccuga cucccguggg gagauuaaua acagcaaauc     1680

cugucaucac ggaaaguacc gagaacucca agaugaugcu ggaacuagau ccacccuuug     1740

gcgacagcua cauugugauc ggaguaggcg auaagaaaau aacucaccac uggcauaggu     1800

cugggucaac aauuggaaag gcguucgagg cuaccgugcg gggugccaaa cgcauggcag     1860

uucucggcga cacagccugg gauuucggga gugucggagg cguguuuaau agccugggua     1920

aggggaucca ccagaucuuc ggcgcugccu uuaaaucccu uuucggaggc augucuuggu     1980

uuucgcaaau uuugaucggg acucugcucg uguggcuggg acuuaacacc aagaacggca     2040

gcauuucccu gacguguuug gcacucggug gggucaugau auuccuguca accgcuguua     2100

gugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6060
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 2 opt

<400>  6060
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6061
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 opt

<400>  6061
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagauaucg      180

ugcuggaaca cggcgggugu gucacaguga uggcacaaga caaaccuacu guugauauug      240

aguuggugac gaccacaguc aguaauaugg ccgaagugcg gagcuacugc uaugaggcgu      300

ccaucucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guaugcaaac gaacacuggu ugaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuaua caaccggaga aucuggagua cagaaucaug cuuagcgucc      540

auggauccca gcacucaggu augauuguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu ggaaaucacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuagucc auaaggaaug guuucacgac auuccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuuguug      840

aguucaagga ugcacacgcc aagagacaga cagugguagu guugggcucc caggaagggg      900

cuguccauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ccccgcugag acccugcacg     1080

gcacuguuac aguggaaguc caauaugccg guaccgacgg gccuuguaaa gugccagcuc     1140

agauggccgu agauaugcag acguuaaccc cgguuggccg acugauaaca gcaaaucccg     1200

ugauuacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau cgucauuggc gugggggaaa agaaaaucac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgucagggg cgcgaaacgg auggcagugc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6062
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 opt

<400>  6062
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6063
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opt

<400>  6063
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucggcu augaaaccga ugaggacagg gccaaggugg      600

aagucacgcc caacaguccu cgggcugagg caacucuggg aggguuuggc agcuuggguc      660

ucgauugcga gccacgcacc gggcuggacu ucucugaucu guacuaccuc acaaugaaua      720

acaaacauug guuaguucac aaggaauggu uucaugacau uccccugccu uggcacgccg      780

gagcugacac uggcacccca cacuggaaua acaaagaggc ccuuguggag uucaaggaug      840

cgcaugcaaa gagacagaca guaguggucu ugggcuccca ggaaggggcc gugcacaccg      900

cucuggccgg agcacuagaa gcugagaugg acggugccaa aggcaggcuc uuuucagggc      960

aucugaagug ucggcuuaaa auggauaagc ugcgcuugaa aggaguuagc uauagucucu     1020

gcacggcggc cuucacuuuu acaaaggugc ccgcagagac ccugcacggc acugucacag     1080

uggaaguaca auacgcuggu accgacgggc cuuguaaaau cccaguucag auggccgugg     1140

auaugcagac guuaaccccg gucggccgac ugauuacagc uaaucccgug aucacugagu     1200

cuaccgaaaa cuccaagaug augcucgagc uugacccucc auucggagau agcuauauag     1260

ucauuggcgu gggggacaag aaaaucacac accauuggca cagaucggga ucuacuauug     1320

guaaggccuu ugaagcaacc guuaggggcg ccaaacggau ggcugugcug ggggauacag     1380

cgugggacuu cggauccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6064
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 2 opt

<400>  6064
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6065
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       2 opt

<400>  6065
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcugagug gaaagggcuc ucuagucaca ugcgcgaaau      960

ucgccugcuc caagaaaaug accggcaaga gcauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcuguc ggugcacggg ucucaacacu ccggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucacccaga gcugaagcca     1140

cucuuggcgg guuuggaagu cugggcuugg acugugagcc uaggaccggu cucgauuuca     1200

gcgaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguuuc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaguuc aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaucccagga aggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaagcuga     1500

gacugaaagg ggugucauau aguuugugua cugccgcuuu uacguucaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagucca ccgaaaacag caagaugaug cuggagcuag     1740

aucccccguu uggcgacucu uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucagggagu acaauuggaa aggcauucga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuuugg gagcguagga ggcgcucuua     1920

auucccuugg uaaggggauc caccagaucu ucggcgccgc guucaaaucu uuguuuggag     1980

gcaugucgug guucagccaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cuccauuuca uugauguguc ucgcccuggg ugggguguua auauuucuga     2100

guacggcugu uucugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6066
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 2
       opt

<400>  6066
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac acuugugaug      240

ccaccauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa cacaaccagu acuugggugg ucuauggcac augucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugaccc uaccguccca uucuacgagg aaacuccaaa      420

cucggucgca gaccuggcug gagucacgcg aguacacaaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac uucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

ccuggguuga uguggucuua gaacauggug gcugugugac agugauggcu caggacaagc      720

caaccgucga uauugagcug guuacgacua cagugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acccaagggg      840

aggccuaccu ugacaagcag agcgacacuc aguaugugug caaaaggaca uuggucgauc      900

ggggaugggg caaugguugu gggcugagug gaaagggcuc ucuagugacc ugcgcgaaau      960

ucgccugcuc caagaaaaug acgggcaaga gcauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcuguc gguucacggg ucucaacacu ccggaaugau cgugaaugac accggucaug     1080

aaacagauga gaaccgcgca aaggucgaga uuacuccaaa uucacccaga gcugaagcca     1140

cccuuggcgg guuuggaagu cugggcuugg acugugagcc uaggacaggu cucgauuuca     1200

gcgaccugua uuacuuaacu augaacaaua aacacuggcu ggugcauaag gaaugguuuc     1260

acgauauccc acucccgugg cacgcugggg ccgacaccgg cacaccccau uggaacaaua     1320

aagaggcacu uguagaguuc aaggaugccc acgcuaaacg gcagaccguu gugguccugg     1380

gaucccagga aggcgcggug cauacugcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaagcuga     1500

gacugaaagg ggucucauau aguuugugua cggccgcuuu uacauucacc aagaucccug     1560

ccgagacucu ccauggaacc gugacaguug aagugcagua cgcaggcacc gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacucu gacaccugug gggagguuaa     1680

uuacggccaa cccaguuaua accgagucca cugaaaacag caagaugaug cuggagcuag     1740

aucccccguu uggcgacucu uacaucguga uuggagucgg cgagaaaaag aucacacacc     1800

acuggcaucg gucagggagu accauaggaa aggcauucga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauacugcau gggacuuugg gagcguggga ggcgcucuua     1920

auucccuugg uaaggggauu caccagaucu ucggcgccgc guucaaaucu uuguuuggag     1980

gcaugucgug guucagccaa auccugauug ggacccuccu gauguggcuu ggacugaacg     2040

ccaagaaugg cuccaucuca uugauguguc ucgcucuggg ugggguuuua auuuuucuga     2100

guacggcagu cucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6067
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 2 
       opt

<400>  6067
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauu aagguggaga      480

acuggaucuu uagaaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauuag gugcaucggc guauccaacc gggacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcacggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgcagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaagaacu uugguggaua      900

ggggaugggg caacgguugu gggcugucug gaaagggcuc ccuaguuaca ugcgccaaau      960

uuaccugcag caagaagaug acgggcaaau cgauccagcc cgaaaaucuc gaauaucgga     1020

uuaugcuguc ugugcacggg ucccaacauu caggaaugau cgguuacgag accgacgaag     1080

aucgugcgaa ggucgaggug acaccuaaca guccacgcgc cgaggcaacu cuuggcgggu     1140

ucggaagccu gggcuuggac ugugaaccca gaaccggucu cgauuucucc gaccuguauu     1200

acuuaacaau gaauaacaaa cacuggcugg uacauaagga gugguuucac gauaucccuc     1260

ucccauggca cgcuggggcc gacacuggca ccccgcauug gaauaacaaa gaagcucuug     1320

uugaguucaa ggaugcccac gcaaaaaggc agacaguggu cgugcuggga ucucaggaag     1380

gcgccgucca uaccgcuuug gcgggggcac uagaggccga gauggacgga gcuaaggguc     1440

ggcuguuuag cggccaccuc aagugccgac ugaaaaugga uaaacuucgc cugaaggggg     1500

ugucauauag uuuguguacu gccgcauuca cguuuacaaa gguucccgcc gaaacccucc     1560

auggaacugu gaccguagag guccaguacg cuggcacaga cgguccuugc aaaauuccag     1620

ugcaaauggc cguugacaug cagacccuga cucccguggg gagauuaaua acagcaaauc     1680

cugucaucac ggaauccacc gagaacagca agaugaugcu ggaacuagau ccacccuucg     1740

gcgacucuua cauugugauc ggaguaggcg auaagaaaau aacucaccac uggcauaggu     1800

cagggaguac aauuggaaag gcguuugagg cuaccgugcg gggugccaaa cgcauggcag     1860

uucucggcga cacagccugg gauuucggga gcgucggagg cguguucaau ucccugggua     1920

aggggaucca ccagaucuuu ggcgcugccu ucaaaucucu uuuuggaggc augucguggu     1980

ucagccaaau uuugaucggg acucugcucg uguggcuggg acuuaacacc aagaacggcu     2040

ccauuucacu gacguguuug gcacucggug gggucaugau auuucugagu accgcuguuu     2100

cugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6068
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 2 
       opt

<400>  6068
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcugagug gaaagggcuc ucuagugaca ugcgcgaaau      960

ucgccugcuc caagaaaaug accggcaaga gcauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcuguc gguucacggg ucucaacacu ccggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucacccaga gcugaagcca     1140

cucuuggcgg guuuggaagu cugggcuugg acugugagcc uaggaccggu cucgauuuca     1200

gcgaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguuuc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaguuc aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaucccagga aggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaagcuga     1500

gacugaaagg ggucucauau aguuugugua cugccgcuuu uacguucaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagucca ccgaaaacag caagaugaug cuggagcuag     1740

aucccccguu uggcgacucu uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucagggagu acaauaggaa aggcauucga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuuugg gagcguggga ggcgcucuua     1920

auucccuugg uaaggggauu caccagaucu ucggcgccgc guucaaaucu uuguuuggag     1980

gcaugucgug guucagccaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cuccaucuca uugauguguc ucgcccuggg ugggguuuua auuuuucuga     2100

guacggcugu cucugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6069
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       2 opt

<400>  6069
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguucaggac acgggucaug     1080

aaaccgauga gaaucgcgca aagguggaga ucacaccaaa cucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaauaaca aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaauaaca     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucaaua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcagaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa uccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

acagccuugg uaaggggauc caccaaaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcag auucugaucg ggacucuccu gauguggcuu ggacugaaua     2040

ccaagaacgg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6070
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 2
       opt

<400>  6070
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac acuugugaug      240

ccaccauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa cacaaccagu acuugggugg ucuauggcac augucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugaccc uaccguccca uucuacgagg aaacuccaaa      420

cucggucgca gaccuggcug gagucacgcg aguacacaaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac uucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

ccuggguuga uguggucuua gaacauggug gcugugugac agugauggcu caggacaagc      720

caaccgucga uauugagcug guuacgacua cagugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acccaagggg      840

aggccuaccu ugacaagcag agcgacacuc aguaugugug caaaaggaca uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugacc ugcgcgaaau      960

uugccugcuc uaagaaaaug acgggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugcaggac accggucaug     1080

aaacagauga gaaucgcgca aaggucgaga uuacuccaaa cucccccaga gcugaagcca     1140

cccuuggcgg guucggauca cugggcuugg acugugagcc uaggacaggu cucgauuuua     1200

gugaccugua uuacuuaacu augaauaaca aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacaccgg cacaccccau uggaauaaca     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagaccguu gugguccugg     1380

gaagccagga gggcgcggug cauacugcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cggccgcuuu cacauuuacc aagaucccug     1560

ccgagacucu ccauggaacc gugacaguug aagugcaaua cgcaggcacc gacgguccau     1620

gcaaaguacc cgcgcagaug gcugucgaca ugcagacucu gacaccugug gggagguuaa     1680

uuacggccaa uccaguuaua accgagagua cugaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacacacc     1800

acuggcaucg gucuggguca accauaggaa aggcauuuga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauacugcau gggacuucgg gaguguggga ggcgcucuga     1920

acagccuugg uaaggggauu caccaaaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcag auccugauug ggacccuccu gauguggcuu ggacugaaug     2040

ccaagaacgg cagcaucucc uugauguguc ucgcucuggg ugggguuuua auuuuccugu     2100

caacggcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6071
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 2 
       opt

<400>  6071
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugcaggac acgggucaug     1080

aaaccgauga gaaucgcgca aaggucgaga uuacaccaaa cucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaauaaca aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaauaaca     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcaaua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcagaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa uccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

acagccuugg uaaggggauu caccaaaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcag auccugauug ggacucuccu gauguggcuu ggacugaaua     2040

ccaagaacgg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6072
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6072
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acacucggaa aggcauuuag uaccacacug aaaggugccc     1860

agcgccuugc ugccuugggc gauacugcau gggacuucgg gagcauugga ggcgucuuca     1920

auuccaucgg uagagcugug caccaaguau uugggggcgc cuucaggacc cuguuuggag     1980

gcaugucuug gaucacgcag gggcucaugg gagcgcugcu ucuguggaug ggcgugaacg     2040

cccgugaucg gucgauugcu uuggcauucc ucgccaccgg ugggguucug gucuuuuuag     2100

cuacaaaugu gcaugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6073
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6073
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac acuugugaug      240

ccaccauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa cacaaccagu acuugggugg ucuauggcac augucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugaccc uaccguccca uucuacgagg aaacuccaaa      420

cucggucgca gaccuggcug gagucacgcg aguacacaaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac uucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

ccuggguuga uguggucuua gaacauggug gcugugugac agugauggcu caggacaagc      720

caaccgucga uauugagcug guuacgacua cagugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acccaagggg      840

aggccuaccu ugacaagcag agcgacacuc aguaugugug caaaaggaca uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugacc ugcgcgaaau      960

uugccugcuc uaagaaaaug acgggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac accggucaug     1080

aaacagauga gaaccgcgca aaggucgaga uuacuccaaa uucccccaga gcugaagcca     1140

cccuuggcgg guucggauca cugggcuugg acugugagcc uaggacaggu cucgauuuua     1200

gugaccugua uuacuuaacu augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacaccgg cacaccccau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagaccguu gugguccugg     1380

gaagccagga gggcgcggug cauacugcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cggccgcuuu cacauuuacc aagaucccug     1560

ccgagacucu ccauggaacc gugacaguug aagugcagua cgcaggcacc gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacucu gacaccugug gggagguuaa     1680

uuacggccaa cccaguuaua accgagagua cugaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacacacc     1800

acuggcaucg gucuggguca acccucggaa aggcauuuag uacaacucug aaaggugccc     1860

agcgccuugc ugccuugggc gauaccgcau gggacuucgg gagcauagga ggcguguuca     1920

auuccauugg uagagcugua caccaagugu uugggggcgc cuucaggacg cuguuuggag     1980

gcaugucuug gaucacccag gggcucaugg gagcgcugcu ucuguggaug ggcguuaacg     2040

cccgugaucg gucgaucgcu uuggcauucc ucgccacagg ugggguccug guguuuuuag     2100

cuacuaaugu gcaugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6074
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 2 opt

<400>  6074
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauu aagguggaga      480

acuggaucuu uagaaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauuag gugcaucggc guauccaacc gggacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcacggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgcagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaagaacu uugguggaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauccagcc cgaaaaucuc gaauaucgga     1020

uuaugcuguc ggugcacggg ucucaacauu ccggaaugau cgguuacgag accgacgaag     1080

aucgugcgaa ggucgaggug acaccuaacu caccacgcgc cgaggcaacu cuuggcgggu     1140

ucggaagucu gggcuuggac ugugaaccca gaaccggucu cgauuuuagc gaccuguauu     1200

acuuaacaau gaauaacaaa cacuggcugg uacauaagga gugguuccac gauaucccuc     1260

ucccauggca cgcuggggcc gacacuggca ccccgcauug gaauaacaaa gaagcucuug     1320

uugaguuuaa ggaugcccac gcaaaaaggc agacaguggu cgugcuggga ucccaggaag     1380

gcgccgucca uaccgcuuug gcgggggcac uagaggccga gauggacgga gcuaaggguc     1440

ggcuguucuc uggccaccuc aagugccgac ugaaaaugga uaaacuucgc cugaaggggg     1500

ugagcuauuc auuguguacu gccgcauuua cguucacaaa gguucccgcc gaaacccucc     1560

auggaacugu gaccguagag guccaguacg cuggcacaga cgguccuugc aaaauuccag     1620

ugcaaauggc cguugacaug cagacccuga cucccguggg gagauuaaua acagcaaauc     1680

cugucaucac ggaaaguacc gagaacucca agaugaugcu ggaacuagau ccacccuuug     1740

gcgacagcua cauugugauc ggaguaggcg auaagaaaau aacucaccac uggcauaggu     1800

cugggucaac acucggaaag gcguucagua ccacacugaa aggugcucag cggcuugccg     1860

cauugggcga cacugccugg gauuucggga gcauuggagg cguguuuaau uccaucgguc     1920

gcgcuguuca ccaagucuuc gggggcgccu uuagaacccu guucggaggc augucuugga     1980

ucacgcaggg gcucauggga gcacugcuuc uguggauggg cgugaacgcu agggaccguu     2040

cgauugccuu ggcguuucuc gccaccggug gggugcuggu cuucuuagcu acaaacguuc     2100

augcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6075
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6075
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acacucggaa aggcauuuag uaccacacug aaaggugccc     1860

agcgccuugc ugccuugggc gauacugcau gggacuucgg gagcauagga ggcguguuca     1920

auuccauugg uagagcugua caccaagugu uugggggcgc cuucaggacc cuguuuggag     1980

gcaugucuug gaucacgcag gggcucaugg gagcgcugcu ucuguggaug ggcguuaacg     2040

cccgugaucg gucgaucgcu uuggcauucc ucgccaccgg ugggguccug guguuuuuag     2100

cuacaaaugu gcaugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6076
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  6076
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacauag      720

uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca guggacauug      780

agcuggucac cacgacuguu ucaaauaugg cugaagugcg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu ggaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuugugg     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguaguugu gcugggcagc caggaggggg     1440

ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggaa acccuccaug     1620

gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag acccugacuc cugucggcag guuaauuaca gccaacccag     1740

ugaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggacac agccugggau uucggaagug uaggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag ugcugauuuu ccugucaacg gccguuagug     2160

cugacgucgg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6077
<211>  2521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6077
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uuaacgccag aaaggagaaa aagaggcggg gcgcugacac cagcgugggg auagucggac      120

ugcuccuuac aacugcaaug gccgcggaag uuacgcgccg agguuccgcu uacuauaugu      180

accuggaucg uaaugacgcc ggcgaggcaa ucucuuuccc caccacauug gggaugaaca      240

aaugcuauau ucagaucaug gauuuaggac acauguguga cgccacuaug ucauacgaau      300

gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc aauaccacaa      360

guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggcuaga aggagccggc      420

gcgcagugac acuaccgucc cauucuacca gaaagcucca aacgaggucg cagacuuggc      480

uggagucacg ggaauacacc aagcaccuua uucgcguuga gaacuggauc uuucgaaauc      540

ccggauucgc ccuggcugcc gcggcaauag ccugguugcu cggcagcagu acaucccaga      600

aagugaucua ucuggucaug auucugcuca ucgcuccugc cuacucuauu agaugcaucg      660

gcgugagcaa cagggauuuu guagaaggga uguccggagg uacuuggguu gacauagugu      720

uagagcaugg cggguguguc accgugaugg cacaggauaa gccaacagug gacauugagc      780

uggucaccac gacuguuuca aauauggcug aagugcggag uuacugcuau gaggccagca      840

ucucugauau ggcguccgac ucacguuguc ccacacaagg agaagccuac cuugacaaac      900

agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg gguaacgggu      960

guggacuguu cggcaaaggc agucuaguca caugcgcaaa guuugcuugc ucuaagaaaa     1020

ugaccgggaa guccauucag ccugagaauc ucgaauacag gaucaugcug agcgugcacg     1080

gaucgcaaca cucugguaug aucguuaacg auacgggcca ugagaccgac gagaaucggg     1140

ccaaagugga aauuacacca aacucacccc gagcugaggc cacucuuggg ggauucggcu     1200

cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug uauuacuuaa     1260

caaugaauaa caagcacugg cugguccaua aagagugguu ccacgacaua ccacucccgu     1320

ggcacgcagg cgccgauacu ggaacacccc auuggaauaa caaggaagcu cuuguggagu     1380

uuaaagacgc gcacgcaaag agacagaccg uaguugugcu gggcagccag gagggggccg     1440

uccauaccgc uuuggccgga gcacuagaag ccgagaugga uggugcuaag ggcaggcugu     1500

ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag ggagugagcu     1560

auucauugug uacugccgca uucacguuua caaaaauccc ugcggaaacc cuccauggca     1620

cugucaccgu ggagguucag uacgcuggua cagacgggcc augcaaggug cccgcccaaa     1680

uggcaguaga uaugcagacc cugacuccug ucggcagauu aauuacagcc aacccaguga     1740

ucacggaaag uaccgaaaau uccaagauga ugcuggagcu agaucccccg uucggagaca     1800

gcuacauagu uauuggcgug ggggagaaaa agaucacuca ccacuggcau aggucuggau     1860

caacaaucgg uaaggcuuuu gaggccaccg uccguggcgc aaaacggaug gcugugcucg     1920

gggacacagc cugggauuuc ggaaguguag gcggugcgcu gaacagccuu gggaaaggca     1980

uucaccagau cuucggagcc gcuuuuaagu ccuuguucgg cgggaugucu ugguuuucgc     2040

aaauucugau aggaacucuc cugauguggc uuggccugaa uaccaagaac gguagcaucu     2100

ccuugaugug ucucgcauua gggggagugc ugauuuuccu gucaacggcc guuagugcug     2160

acgucggcug cucuguggau uuuagcaaau gaggacuagu gcaucacauu uaaaagcauc     2220

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2280

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2340

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2460

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2520

u                                                                     2521


<210>  6078
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6078
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaaaaag aggcggggcg cugacaccag cguggggauu gucggacugc      120

uccuuacaac ugcaauggcc gcggaaguua cgcgccgagg uuccgcuuac uauauguacc      180

uggaucguaa ugacgccggc gaggcaauau cuuuccccac cacauugggg augaacaaau      240

gcuauaucca gauuauggau uuaggacaca ugugugacgc cacuauguca uacgaaugcc      300

cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau accacaagua      360

ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuagaagg agccggcgcg      420

cagugacacu accgucccau ucuaccagaa agcuccaaac gaggucgcag acuuggcugg      480

agucacggga auacaccaag caccuuaucc gcguugagaa cuggauuuuu cgaaaucccg      540

gauucgcccu ggcugccgcg gcaaucgccu gguugcucgg cagcaguaca ucccagaaag      600

ugauauaucu ggucaugauc cugcucauug cuccugccua cucuaucaga ugcauuggcg      660

ugagcaacag ggauuuugua gaagggaugu ccggagguac uuggguugac aucguguuag      720

agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac auagagcugg      780

ucaccacgac uguuucaaau auggcugaag ugcggaguua cugcuaugag gccagcauuu      840

cugauauggc guccgacuca cguuguccca cacaaggaga agccuaccuu gacaaacaga      900

gcgauaccca guauguaugc aagcgcacuu ugguggacag aggcuggggu aacgggugug      960

gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu aagaaaauga     1020

ccgggaaguc cauccagccu gagaaucucg aauacaggau uaugcugagc gugcacggau     1080

cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag aaucgggcca     1140

aaguggaaau cacaccaaac ucaccccgag cugaggccac ucuuggggga uucggcuccc     1200

uggguuugga uugugaaccu cgcaccgggc ucgacuuuag ugaucuguau uacuuaacaa     1260

ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauucca cucccguggc     1320

acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu guggaguuua     1380

aagacgcgca cgcaaagaga cagaccguag uugugcuggg cagccaggag ggggccgucc     1440

auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc aggcuguccu     1500

cugggcaccu caaaugccgg cugaagaugg acaaacuucg ccugaaggga gugagcuauu     1560

cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc cauggcacug     1620

ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc gcccaaaugg     1680

caguagauau gcagacccug acuccugucg gcagauuaau cacagccaac ccagugauua     1740

cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc ggagacagcu     1800

acaucguuau aggcgugggg gagaaaaaga uuacucacca cuggcauagg ucuggaucaa     1860

caaucgguaa ggcuuuugag gccaccgucc guggcgcaaa acggauggcu gugcucgggg     1920

acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg aaaggcaucc     1980

accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg uuuucgcaaa     2040

uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu agcauauccu     2100

ugaugugucu cgcauuaggg ggagugcuga ucuuccuguc aacggccguu agugcugacg     2160

ucggcugcuc uguggauuuu agcaaaugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  6079
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6079
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaaaaagagg cggggcgcug acaccagcgu ggggaucguc ggacugcucc      120

uuacaacugc aauggccgcg gaaguuacgc gccgagguuc cgcuuacuau auguaccugg      180

aucguaauga cgccggcgag gcaauuucuu uccccaccac auuggggaug aacaaaugcu      240

auauacagau cauggauuua ggacacaugu gugacgccac uaugucauac gaaugcccua      300

ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaauacc acaaguaccu      360

ggguggucua ugguacuugu caucacaaga aaggggaggc uagaaggagc cggcgcgcag      420

ugacacuacc gucccauucu accagaaagc uccaaacgag gucgcagacu uggcuggagu      480

cacgggaaua caccaagcac cuuauucgcg uugagaacug gaucuuucga aaucccggau      540

ucgcccuggc ugccgcggca auugccuggu ugcucggcag caguacaucc cagaaaguga      600

ucuaucuggu caugauacug cucaucgcuc cugccuacuc uauuagaugc aucggcguga      660

gcaacaggga uuuuguagaa gggauguccg gagguacuug gguugacauu guguuagagc      720

auggcgggug ugucaccgug auggcacagg auaagccaac aguggacauc gagcugguca      780

ccacgacugu uucaaauaug gcugaagugc ggaguuacug cuaugaggcc agcauaucug      840

auauggcguc cgacucacgu ugucccacac aaggagaagc cuaccuugac aaacagagcg      900

auacccagua uguaugcaag cgcacuuugg uggacagagg cugggguaac ggguguggac      960

uguucggcaa aggcagucua gucacaugcg caaaguuugc uugcucuaag aaaaugaccg     1020

ggaaguccau ucagccugag aaucucgaau acaggaucau gcugagcgug cacggaucgc     1080

aacacucugg uaugauuguu aacgauacgg gccaugagac cgacgagaau cgggccaaag     1140

uggaaaucac accaaacuca ccccgagcug aggccacucu ugggggauuc ggcucccugg     1200

guuuggauug ugaaccucgc accgggcucg acuuuaguga ucuguauuac uuaacaauga     1260

auaacaagca cuggcugguc cauaaagagu gguuccacga caucccacuc ccguggcacg     1320

caggcgccga uacuggaaca ccccauugga auaacaagga agcucuugug gaguuuaaag     1380

acgcgcacgc aaagagacag accguaguug ugcugggcag ccaggagggg gccguccaua     1440

ccgcuuuggc cggagcacua gaagccgaga uggauggugc uaagggcagg cuguccucug     1500

ggcaccucaa augccggcug aagauggaca aacuucgccu gaagggagug agcuauucau     1560

uguguacugc cgcauucacg uuuacaaaaa uuccugcgga aacccuccau ggcacuguca     1620

ccguggaggu ucaguacgcu gguacagacg ggccaugcaa ggugcccgcc caaauggcag     1680

uagauaugca gacccugacu ccugucggca gauuaauaac agccaaccca gugaucacgg     1740

aaaguaccga aaauuccaag augaugcugg agcuagaucc cccguucgga gacagcuaca     1800

uuguuaucgg cgugggggag aaaaagauaa cucaccacug gcauaggucu ggaucaacaa     1860

uugguaaggc uuuugaggcc accguccgug gcgcaaaacg gauggcugug cucggggaca     1920

cagccuggga uuucggaagu guaggcggug cgcugaacag ccuugggaaa ggcauccacc     1980

agaucuucgg agccgcuuuu aaguccuugu ucggcgggau gucuugguuu ucgcaaauuc     2040

ugaucggaac ucuccugaug uggcuuggcc ugaauaccaa gaacgguagc auuuccuuga     2100

ugugucucgc auuaggggga gugcugauau uccugucaac ggccguuagu gcugacgucg     2160

gcugcucugu ggauuuuagc aaaugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6080
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6080
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa aaagaggcgg ggcgcugaca ccagcguggg gaucgucgga cugcuccuua      120

caacugcaau ggccgcggaa guuacgcgcc gagguuccgc uuacuauaug uaccuggauc      180

guaacgacgc cggcgaggca auuucuuucc ccaccacauu ggggaugaau aaaugcuaua      240

uacagaucau ggauuuagga cacaugugug acgccacuau gucauacgaa ugcccuaugc      300

uggaugaggg cguggaacca gacgauguag acuguuggug caacaccaca aguaccuggg      360

uggucuaugg uacuugucau cacaagaaag gggaggcuag aaggagccgg cgcgcaguga      420

cacuaccguc ccauucuacc agaaagcucc aaacgagguc gcagacuugg cuggagucac      480

gggaauacac caagcaccuu auucgcguug agaauuggau cuuucgaaac cccggauucg      540

cccuggcugc cgcggcaauu gccugguugc ucggcagcag uacaucccag aaagugaucu      600

aucuggucau gauacugcuc aucgcuccug ccuacucuau uagaugcauc ggcgugagca      660

auagggauuu uguagaaggg auguccggag guacuugggu ugacauugug uuagagcaug      720

gcgggugugu caccgugaug gcacaggaua agccaacagu ggacaucgag cuggucacca      780

cgacuguuuc aaacauggcu gaagugcgga guuacugcua ugaggccagc auaucugaua      840

uggcguccga cucacguugu cccacacaag gagaagccua ccuugacaaa cagagcgaua      900

cccaguaugu augcaagcgc acuuuggugg acagaggcug ggguaauggg uguggacugu      960

ucggcaaagg cagucuaguc acaugcgcaa aguuugcuug cucuaagaaa augaccggga     1020

aguccauuca gccugagaac cucgaauaca ggaucaugcu gagcgugcac ggaucgcaac     1080

acucugguau gauuguuaau gauacgggcc augagaccga cgagaaccgg gccaaagugg     1140

aaaucacacc aaauucaccc cgagcugagg ccacucuugg gggauucggc ucccuggguu     1200

uggauuguga accucgcacc gggcucgacu uuagugaucu guauuacuua acaaugaaca     1260

auaagcacug gcugguccau aaagaguggu uccacgacau cccacucccg uggcacgcag     1320

gcgccgauac uggaacaccc cauuggaaca auaaggaagc ucuuguggag uuuaaagacg     1380

cgcacgcaaa gagacagacc guaguugugc ugggcagcca ggagggggcc guccauaccg     1440

cuuuggccgg agcacuagaa gccgagaugg auggugcuaa gggcaggcug uccucugggc     1500

accucaaaug ccggcugaag auggacaaac uucgccugaa gggagugagc uauucauugu     1560

guacugccgc auucacguuu acaaaaauuc cugcggaaac ccuccauggc acugucaccg     1620

uggagguuca guacgcuggu acagacgggc caugcaaggu gcccgcccaa auggcaguag     1680

auaugcagac ccugacuccu gucggcagau uaauaacagc caacccagug aucacggaaa     1740

guaccgagaa cuccaagaug augcuggagc uagauccccc guucggagac agcuacauug     1800

uuaucggcgu gggggagaaa aagauaacuc accacuggca uaggucugga ucaacaauug     1860

guaaggcuuu ugaagccacc guccguggcg caaaacggau ggcugugcuc ggggacacag     1920

ccugggauuu cggaagugua ggcggugcgc ugaauagccu ugggaaaggc auccaccaga     1980

ucuucggagc cgcuuuuaag uccuuguucg gcgggauguc uugguuuucg caaauucuga     2040

ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu uccuugaugu     2100

gucucgcauu agggggagug cugauauucc ugucaacggc cguuagugcu gacgucggcu     2160

gcucugugga uuuuagcaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6081
<211>  2509
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6081
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaaaaa gaggcggggc gccgacacca gcguggggau cgucggacug cuccuuacaa      120

cugcuauggc agccgaaguu acgcgccgag guuccgcgua cuauauguac cuggaucgua      180

acgacgcugg cgaggccauu ucuuucccca ccacauuggg gaugaauaaa ugcuauauac      240

agaucaugga uuuaggacac augugugacg caacuauguc auacgaaugc ccuaugcugg      300

augagggcgu ggaaccagac gauguagacu guuggugcaa caccacaagu accugggugg      360

ucuaugguac uugucaucac aagaaagggg aggccagaag gagccggcgc gcugugacac      420

uaccguccca uucuaccaga aagcuccaaa cgaggucgca gacuuggcug gagucacggg      480

aauacaccaa gcaccuuauu cgcguugaga auuggaucuu ucgaaacccc ggauucgcac      540

uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaaa gugaucuauc      600

uggucaugau acugcucauc gccccugcuu acucuauuag augcaucggc gugagcaaua      660

gggauuuugu agaagggaug uccggaggua cuuggguuga cauuguguua gagcauggcg      720

ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug gucaccacga      780

cuguuucaaa cauggcagaa gugcggaguu acugcuauga ggcuagcaua ucugauaugg      840

ccuccgacuc acguuguccc acacaaggag aagcguaccu ugacaaacag agcgauaccc      900

aguauguaug caagcgcacu uugguggaca gaggcugggg uaaugggugu ggacuguucg      960

gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaagaaaaug accgggaagu     1020

ccauucagcc ugagaaccuc gaauacagga ucaugcugag cgugcacgga ucgcaacacu     1080

cugguaugau uguuaaugau acgggccaug agaccgacga gaaccgggcu aaaguggaaa     1140

ucacaccaaa uucaccccga gccgaggcua cucuuggggg auucggcucc cuggguuugg     1200

auugugaacc ucgcaccggg cucgacuuua gugaucugua uuacuuaaca augaacaaua     1260

agcacuggcu gguccauaaa gagugguucc acgacauccc acucccgugg cacgccggcg     1320

cagauacugg aacaccccau uggaacaaua aggaagcccu uguggaguuu aaagacgcuc     1380

acgcgaagag acagaccgua guugugcugg gcagccagga gggggcaguc cauaccgccu     1440

uggcuggagc ccuagaagca gagauggaug gugccaaggg caggcugucc ucugggcacc     1500

ucaaaugccg gcugaagaug gacaaacuuc gccugaaggg agugagcuau ucauugugua     1560

cugcugccuu cacguuuaca aaaauuccug cagaaacccu ccauggcacu gucaccgugg     1620

agguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug gccguagaua     1680

ugcagacccu gacuccuguc ggcagauuaa uaacagcaaa cccagugauc acggaaagua     1740

ccgagaacuc caagaugaug cuggagcuag aucccccguu cggagacagc uacauuguua     1800

ucggcguggg ggagaaaaag auaacucacc acuggcauag gucuggauca acaauuggua     1860

aagccuuuga agcuaccguc cguggcgcca agcggauggc agugcucggg gacacagcuu     1920

gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc caccagaucu     1980

ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa auucugaucg     2040

gaacucuccu gauguggcuu ggccugaaca ccaagaaugg uagcauuucc uugauguguc     2100

ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac gucggcugcu     2160

cuguggauuu uagcaaauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2220

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2280

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2340

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2460

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2509


<210>  6082
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6082
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaaaaagag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc cuuacaacug      120

cuauggcagc cgaaguuacg cggcgcgguu ccgcguacua uauguaccug gaucgaaacg      180

acgcuggcga ggccauuucu uuccccacca cauuggggau gaauaaaugc uauauacaga      240

ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgaaugcccu augcuggaug      300

agggcgugga accagacgau guagacuguu ggugcaacac cacaaguacc uggguggucu      360

augguacuug ucaucacaag aaaggggagg cccguagaag caggcgggcu gugacacuac      420

cgucccauuc uacccgcaag cuccaaacga gaucgcagac uuggcuggag ucaagggaau      480

acaccaagca ccuuauucgg guugagaauu ggaucuuucg caaccccgga uucgcacugg      540

ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug aucuaucugg      600

ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug agcaauagag      660

auuuuguaga agggaugucc ggagguacuu ggguugacau uguguuagag cauggcgggu      720

gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc accacgacug      780

uuucaaacau ggcagaagug aggaguuacu gcuaugaggc uagcauaucu gauauggccu      840

ccgacucacg gugucccaca caaggagaag cguaccuuga caaacagagc gauacccagu      900

auguaugcaa gcguacuuug guggaccgcg gcugggguaa ugggugugga cuguucggca      960

aaggcagucu agucacaugc gccaaguuug caugcucuaa gaaaaugacc gggaagucca     1020

uucagccuga gaaccucgaa uacagaauca ugcugagcgu gcacggaucg caacacucug     1080

guaugauugu uaaugauacg ggccaugaga ccgacgagaa cagggcuaaa guggaaauca     1140

caccaaauuc accccgggcc gaggcuacuc uugggggauu cggcucccug gguuuggauu     1200

gugaaccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug aacaauaagc     1260

acuggcuggu ccauaaagag ugguuccacg acaucccacu cccguggcac gccggcgcag     1320

auacuggaac accccauugg aacaauaagg aagcccuugu ggaguuuaaa gacgcucacg     1380

cgaagcgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau accgccuugg     1440

cuggagcccu agaagcagag auggauggug ccaagggcag acuguccucu gggcaccuca     1500

aaugcaggcu gaagauggac aaacuucggc ugaagggagu gagcuauuca uuguguacug     1560

cugccuucac guuuacaaaa auuccugcag aaacccucca uggcacuguc accguggagg     1620

uucaguacgc ggguacagac gggccaugca aggugcccgc ucaaauggcc guagauaugc     1680

agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg gaaaguaccg     1740

agaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac auuguuaucg     1800

gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca auugguaaag     1860

ccuuugaagc uaccgucagg ggcgccaagc guauggcagu gcucggggac acagcuuggg     1920

auuucggaag uguaggcggu gcccugaaua gccuugggaa aggcauccac cagaucuucg     1980

gagcggccuu uaaguccuug uucggcggga ugucuugguu uucgcaaauu cugaucggaa     2040

cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug augugucucg     2100

cuuuaggggg agugcugaua uuccugucaa cggcaguuag ugccgacguc ggcugcucug     2160

uggauuuuag caaaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6083
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6083
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagaag gggcgccgac accagcgugg ggaucgucgg acugcuccuu acaacugcua      120

uggcagccga aguuacgcgg cgcgguuccg cguacuauau guaccuggau cgaaacgacg      180

cuggcgaggc cauuucuuuc cccaccacau uggggaugaa uaagugcuau auacagauca      240

uggauuuagg acacaugugu gacgcaacua ugucauacga augcccuaug cuggaugagg      300

gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg guggucuaug      360

guacuuguca ucacaaaaag ggggaggccc guagaagcag gcgggcugug acacuaccgu      420

cccauucuac ccgcaaacuc caaacgagau cgcagacuug gcuggaguca agggaauaca      480

ccaagcaccu uauucggguu gagaauugga ucuuucgcaa ccccggauuc gcacuggccg      540

cugccgcgau ugcaugguug cucggcagca guacauccca gaaggugauc uaucugguca      600

ugauacugcu caucgccccu gcuuacucua uucgaugcau cggcgugagc aauagagauu      660

uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau ggcgggugug      720

ucaccgugau ggcccaggau aaaccaacag uggacaucga gcuggucacc acgacuguuu      780

caaacauggc agaagugagg aguuacugcu augaggcuag cauaucugau auggccuccg      840

acucacggug ucccacacaa ggagaagcgu accuugacaa gcagagcgau acccaguaug      900

uaugcaaacg uacuuuggug gaccgcggcu gggguaaugg guguggacug uucggcaagg      960

gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa gaugaccggg aaauccauuc     1020

agccugagaa ccucgaauac agaaucaugc ugagcgugca cggaucgcaa cacucuggua     1080

ugauuguuaa ugauacgggc caugagaccg acgagaacag ggcuaaggug gaaaucacac     1140

caaauucacc ccgggccgag gcuacucuug ggggauucgg cucccugggu uuggauugug     1200

aaccucgaac cgggcucgac uuuagugauc uguauuacuu aacaaugaac aauaaacacu     1260

ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgcc ggcgcagaua     1320

cuggaacacc ccauuggaac aauaaagaag cccuugugga guuuaaggac gcucacgcga     1380

aacgccagac cguaguugug cugggcagcc aggagggggc aguccauacc gccuuggcug     1440

gagcccuaga agcagagaug gauggugcca agggcagacu guccucuggg caccucaagu     1500

gcaggcugaa aauggacaag cugcggcuga aaggagugag cuauucauug uguacugcug     1560

ccuucacguu uacaaagauu ccugcagaaa cccuccaugg cacugucacc guggagguuc     1620

aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua gauaugcaga     1680

cccugacucc ugucggccgc uuaauaacag caaacccagu gaucacggaa aguaccgaga     1740

acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu guuaucggcg     1800

ugggggaaaa gaaaauaacu caccacuggc auagaucugg aucaacaauu gguaaggccu     1860

uugaggcuac cgucaggggc gccaaacgua uggcagugcu cggggacaca gcuugggauu     1920

ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag aucuucggag     1980

cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug aucggaacuc     2040

uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug ugucucgcuc     2100

uugggggagu guuaauauuc cugucaacgg caguuagugc cgacgucggc ugcucugugg     2160

auuuuagcaa gugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  6084
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6084
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagaagggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca acugcuaugg      120

cagccgaggu uacgcggcgc gguuccgcgu acuauaugua ccuggaucga aacgacgcug      180

gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua cagaucaugg      240

auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug gaugaaggcg      300

uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug gucuauggua      360

cuugucauca caaaaagggg gaagcccgua gaagcaggcg ggcugugaca cuaccguccc      420

auucuacccg caaacuccaa acgagaucgc agacuuggcu ggagucaagg gaguacacca      480

agcaccuuau ucggguugaa aauuggaucu uucgcaaccc cggauucgca cuggccgcug      540

ccgcgauugc augguugcuc ggcagcagua caucccagaa ggugaucuau cuggucauga      600

uacugcucau cgccccugcu uacucuauuc gaugcaucgg cgugagcaau agagauuuug      660

uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc ggguguguca      720

ccgugauggc ccaggauaaa ccaacagugg acaucgagcu ggucaccacg acuguuucaa      780

acauggcaga ggugaggagu uacugcuaug aagcuagcau aucugauaug gccuccgacu      840

cacggugucc cacacaagga gaggcguacc uugacaagca gagcgauacc caguauguau      900

gcaaacguac uuugguggac cgcggcuggg guaaugggug uggacuguuc ggcaagggca      960

gucuagucac augcgccaaa uuugcaugcu cuaagaagau gaccgggaaa uccauucagc     1020

cugaaaaccu cgaguacaga aucaugcuga gcgugcacgg aucgcaacac ucugguauga     1080

uuguuaauga uacgggccau gaaaccgacg agaacagggc uaagguggag aucacaccaa     1140

auucaccccg ggccgaagcu acucuugggg gauucggcuc ccuggguuug gauugugagc     1200

cucgaaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau aaacacuggc     1260

ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc gcagauacug     1320

gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu cacgcgaaac     1380

gccagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc uuggcuggag     1440

cccuagaggc agaaauggau ggugccaagg gcagacuguc cucugggcac cucaagugca     1500

ggcugaaaau ggacaagcug cggcugaaag gagugagcua uucauugugu acugcugccu     1560

ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug gaaguucagu     1620

acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau augcagaccc     1680

ugacuccugu cggccgcuua auaacagcaa acccagugau cacggagagu accgaaaacu     1740

ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu aucggcgugg     1800

gggagaagaa aauaacucac cacuggcaua gaucuggauc aacaauuggu aaggccuuug     1860

aagcuaccgu caggggcgcc aaacguaugg cagugcucgg ggacacagcu ugggauuucg     1920

gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc uucggagcgg     1980

ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc ggaacucucc     2040

ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu cucgcucuug     2100

ggggaguguu aauauuccug ucaacggcag uuagugccga cgucggcugc ucuguggauu     2160

uuagcaagug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  6085
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6085
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gaaggggcgc cgacaccagc guggggaucg ucggacugcu ccuuacaacu gcuauggcag      120

ccgagguuac gcggcgcggu uccgcguacu auauguaccu ggaucgaaac gacgcuggcg      180

aagccauuuc uuuccccacc acauugggga ugaauaaaug cuauauacag aucauggauu      240

uaggacacau gugugacgca acuaugucau acgagugccc uaugcuggau gaaggcgugg      300

agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc uaugguacuu      360

gucaucacaa gaaaggggaa gcccguagaa gcaggcgggc ugugacacua ccgucccauu      420

cuacccgcaa gcuccaaacg agaucgcaga cuuggcugga gucaagggag uacaccaaac      480

accuuauucg gguugaaaau uggaucuuuc gcaaccccgg auucgcacug gccgcugccg      540

cgauugcaug guugcucggc agcaguacau cccagaaggu gaucuaucug gucaugauac      600

ugcucaucgc cccugcuuac ucuauucgau gcaucggcgu gagcaauaga gauuuuguag      660

aggggauguc cggagguacu uggguugaca uuguguuaga acauggcggg ugugucaccg      720

ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu guuucaaaca      780

uggcagaggu gaggaguuac ugcuaugaag cuagcauauc ugauauggcc uccgacucac      840

ggugucccac acaaggagag gcguaccuug acaaacagag cgauacccag uauguaugca      900

agcguacuuu gguggaccgc ggcuggggua augggugugg acuguucggc aaaggcaguc      960

uagucacaug cgccaaguuu gcaugcucua aaaagaugac cgggaagucc auucagccug     1020

aaaaccucga guacagaauc augcugagcg ugcacggauc gcaacacucu gguaugauug     1080

uuaaugauac gggccaugaa accgacgaga acagggcuaa aguggagauc acaccaaauu     1140

caccccgggc cgaagcuacu cuugggggau ucggcucccu ggguuuggau ugugagccuc     1200

gaaccgggcu cgacuuuagu gaucuguauu acuuaacaau gaacaauaag cacuggcugg     1260

uccauaaaga augguuccac gacaucccac ucccguggca cgccggcgca gauacuggaa     1320

caccccauug gaacaauaag gaggcccuug uggaauuuaa agacgcucac gcgaagcgcc     1380

agaccguagu ugugcugggc agccaggagg gggcagucca uaccgccuug gcuggagccc     1440

uagaggcaga aauggauggu gccaaaggca gacuguccuc ugggcaccuc aagugcaggc     1500

ugaagaugga caaacuucgg cugaagggag ugagcuauuc auuguguacu gcugccuuca     1560

cguuuacaaa aauuccugca gagacccucc auggcacugu caccguggaa guucaguacg     1620

cggguacaga cgggccaugc aaggugcccg cucaaauggc cguagauaug cagacccuga     1680

cuccugucgg ccgcuuaaua acagcaaacc cagugaucac ggagaguacc gaaaacucca     1740

aaaugaugcu ggagcuagau cccccguucg gagacagcua cauuguuauc ggcguggggg     1800

agaagaagau aacucaccac uggcauagau cuggaucaac aauugguaaa gccuuugaag     1860

cuaccgucag gggcgccaag cguauggcag ugcucgggga cacagcuugg gauuucggaa     1920

guguaggcgg ugcccugaau agccuuggga aaggcaucca ccagaucuuc ggagcggccu     1980

uuaaguccuu guucggcggg augucuuggu uuucgcaaau ucugaucgga acucuccuga     2040

uguggcuugg ccugaacacc aaaaauggua gcauuuccuu gaugugucuc gcuuuagggg     2100

gagugcugau auuccuguca acggcaguua gugccgacgu cggcugcucu guggauuuua     2160

gcaagugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6086
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6086
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ggggcgccga caccagcgug gggaucgucg gacugcuccu uacaacugcu auggcagccg      120

agguuacgcg gcgcgguucc gcguacuaua uguaccugga ucgaaacgac gcuggcgaag      180

ccauuucuuu ccccaccaca uuggggauga auaagugcua uauacagauc auggauuuag      240

gacacaugug ugacgcaacu augucauacg agugcccuau gcuggaugaa ggcguggagc      300

cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau gguacuuguc      360

aucacaaaaa gggggaagcc cguagaagca ggcgggcugu gacacuaccg ucccauucua      420

cccgcaaacu ccaaacgaga ucgcagacuu ggcuggaguc aagggaguac accaagcacc      480

uuauucgggu ugaaaauugg aucuuucgca accccggauu cgcacuggcc gcugccgcga      540

uugcaugguu gcucggcagc aguacauccc agaaagugau cuaucugguc augauacugc      600

ucaucgcccc ugcuuacucu auucgaugca ucggcgugag caauagagau uuuguagagg      660

ggauguccgg agguacuugg guugacauug uguuagaaca uggcgggugu gucaccguga      720

uggcccagga uaagccaaca guggacaucg agcuggucac cacgacuguu ucaaacaugg      780

cagaggugag gaguuacugc uaugaagcua gcauaucuga uauggccucc gacucacggu      840

gucccacaca aggagaggcg uaccuugaca agcagagcga uacccaguau guaugcaaac      900

guacuuuggu ggaccgcggc ugggguaaug gguguggacu guucggcaag ggcagucuag      960

ucacaugcgc caaauuugca ugcucuaaga aaaugaccgg gaaguccauu cagccugaaa     1020

accucgagua cagaaucaug cugagcgugc acggaucgca acacucuggu augauuguua     1080

augauacggg ccaugaaacc gacgagaaca gggcuaaggu ggagaucaca ccaaauucac     1140

cccgggccga agcuacucuu gggggauucg gcucccuggg uuuggauugu gagccucgaa     1200

ccgggcucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac uggcuggucc     1260

auaaggaaug guuccacgac aucccacucc cguggcacgc cggcgcagau acuggaacac     1320

cccauuggaa caauaaagag gcccuugugg aauuuaagga cgcucacgcg aaacgccaga     1380

ccguaguugu gcugggcagc caggaggggg caguccauac cgccuuggcu ggagcccuag     1440

aggcagaaau ggauggugcc aagggcagac uguccucugg gcaccucaaa ugcaggcuga     1500

agauggacaa gcugcggcug aaaggaguga gcuauucauu guguacugcu gccuucacgu     1560

uuacaaagau uccugcagag acccuccaug gcacugucac cguggaaguu caguacgcgg     1620

guacagacgg gccaugcaaa gugcccgcuc aaauggccgu agauaugcag acccugacuc     1680

cugucggccg cuuaauaaca gcaaacccag ugaucacgga gaguaccgaa aacuccaaga     1740

ugaugcugga gcuagauccc ccguucggag acagcuacau uguuaucggc gugggggaga     1800

aaaagauaac ucaccacugg cauagaucug gaucaacaau ugguaaggcc uuugaagcua     1860

ccgucagggg cgccaaacgu auggcagugc ucggggacac agcuugggau uucggaagug     1920

uaggcggugc ccugaauagc cuugggaagg gcauccacca gaucuucgga gcggccuuua     1980

aauccuuguu cggcgggaug ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu     2040

ggcuuggccu gaacaccaag aaugguagca uuuccuugau gugucucgcu cuugggggag     2100

uguuaauauu ccugucaacg gcaguuagug ccgacgucgg cugcucugug gauuuuagca     2160

aaugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6087
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6087
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug gcagccgagg      120

uuacgaggcg ggguuccgcg uacuauaugu accuggaucg caacgacgcu ggcgaagcca      180

uuucuuuccc caccacauug gggaugaaua agugcuauau acagaucaug gauuuaggac      240

acauguguga cgcaacuaug ucauacgagu gcccuaugcu ggaugaaggc guggagccag      300

acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu acuugucauc      360

acaaaaaggg ggaagcccga cguagcagaa gggcugugac acuaccgucc cauucuaccc      420

ggaaacucca aacgcgcucg cagacuuggc uggagucaag agaguacacc aagcaccuua      480

uuaggguuga aaauuggauc uuucggaacc ccggauucgc acuggccgcu gccgcgauug      540

caugguugcu cggcagcagu acaucccaga aagugaucua ucuggucaug auacugcuca      600

ucgccccugc uuacucuauu cgcugcaucg gcgugagcaa ucgagauuuu guagagggga      660

uguccggagg uacuuggguu gacauugugu uagaacaugg cggguguguc accgugaugg      720

cccaggauaa gccaacagug gacaucgagc uggucaccac gacuguuuca aacauggcag      780

aggugagaag uuacugcuau gaagcuagca uaucugauau ggccuccgac ucaagguguc      840

ccacacaagg agaggcguac cuugacaagc agagcgauac ccaguaugua ugcaaacgga      900

cuuuggugga ccguggcugg gguaaugggu guggacuguu cggcaagggc agucuaguca      960

caugcgccaa auuugcaugc ucuaagaaaa ugaccgggaa guccauucag ccugaaaacc     1020

ucgaguaccg caucaugcug agcgugcacg gaucgcaaca cucugguaug auuguuaaug     1080

auacgggcca ugaaaccgac gagaacagag cuaaggugga gaucacacca aauuccccca     1140

gggccgaagc uacucuuggg ggauucggcu cacuggguuu ggauugugag ccucggaccg     1200

ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaaacacugg cugguccaua     1260

aggaaugguu ccacgacauc ccacucccgu ggcacgccgg cgcagauacu ggaacacccc     1320

auuggaacaa uaaagaggcc cuuguggaau uuaaggacgc ucacgcgaaa cgacagaccg     1380

uaguugugcu gggcagccag gagggggcag uccauaccgc cuuggcugga gcccuagagg     1440

cagaaaugga uggugccaag ggccgccugu ccucugggca ccucaaaugc agacugaaga     1500

uggacaagcu gaggcugaaa ggagugagcu auucauugug uacugcugcc uucacguuua     1560

caaagauucc ugcagagacc cuccauggca cugucaccgu ggaaguucag uacgcgggua     1620

cagacgggcc augcaaagug cccgcucaaa uggccguaga uaugcagacc cugacuccug     1680

ucggccgguu aauaacagca aacccaguga ucacggagag uaccgaaaac uccaagauga     1740

ugcuggagcu agaucccccg uucggagaca gcuacauugu uaucggcgug ggggagaaaa     1800

agauaacuca ccacuggcau cgcucuggau caacaauugg uaaggccuuu gaagcuaccg     1860

ucagaggcgc caaaaggaug gcagugcucg gggacacagc uugggauuuc ggaaguguag     1920

gcggugcccu gaauagccuu gggaagggca uccaccagau cuucggagcg gccuuuaaau     1980

ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc cugauguggc     2040

uuggccugaa caccaagaau gguagcauuu ccuugaugug ucucgcucuu gggggagugu     2100

uaauauuccu gucaacggca guuagugccg acgucggcug cucuguggau uuuagcaaau     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6088
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6088
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gucggacugc uccuuacaac ugcuauggca gccgagguua      120

cgagaagggg uuccgcguac uauauguacc uggaucggaa cgacgcuggc gaagccauuu      180

cuuuccccac cacauugggg augaauaagu gcuauauaca gaucauggau uuaggacaca      240

ugugugacgc aacuauguca uacgagugcc cuaugcugga ugaaggcgug gagccagacg      300

auguagacug uuggugcaac accacaagua ccuggguggu cuaugguacu ugucaucaca      360

aaaaggggga agcccgccga agccguagag cugugacacu accgucccau ucuaccagga      420

aacuccaaac gcggucgcag acuuggcugg agucacgcga guacaccaag caccuuauua      480

gaguugaaaa uuggaucuuu aggaaccccg gauucgcacu ggccgcugcc gcgauugcau      540

gguugcucgg cagcaguaca ucccagaaag ugaucuaucu ggucaugaua cugcucaucg      600

ccccugcuua cucuauucgg ugcaucggcg ugagcaaucg cgauuuugua gaggggaugu      660

ccggagguac uuggguugac auuguguuag aacauggcgg gugugucacc gugauggccc      720

aggauaagcc aacaguggac aucgagcugg ucaccacgac uguuucaaac auggcagagg      780

ugcgaaguua cugcuaugaa gcuagcauau cugauauggc cuccgacuca agauguccca      840

cacaaggaga ggcguaccuu gacaagcaga gcgauaccca guauguaugc aaaaggacuu      900

ugguggaccg gggcuggggu aaugggugug gacuguucgg caagggcagu cuagucacau      960

gcgccaaauu ugcaugcucu aagaaaauga ccgggaaguc cauucagccu gaaaaccucg     1020

aguaccguau caugcugagc gugcacggau cgcaacacuc ugguaugauu guuaaugaua     1080

cgggccauga aaccgacgag aaccgcgcua agguggagau cacaccaaau ucccccagag     1140

ccgaagcuac ucuuggggga uucggcucac uggguuugga uugugagccu aggaccgggc     1200

ucgacuuuag ugaucuguau uacuuaacaa ugaacaauaa acacuggcug guccauaagg     1260

aaugguucca cgacauccca cucccguggc acgccggcgc agauacugga acaccccauu     1320

ggaacaauaa agaggcccuu guggaauuua aggacgcuca cgcgaaacgg cagaccguag     1380

uugugcuggg cagccaggag ggggcagucc auaccgccuu ggcuggagcc cuagaggcag     1440

aaauggaugg ugccaagggc cgacuguccu cugggcaccu caaaugccgc cugaagaugg     1500

acaagcugag acugaaagga gugagcuauu cauuguguac ugcugccuuc acguuuacaa     1560

agauuccugc agagacccuc cauggcacug ucaccgugga aguucaguac gcggguacag     1620

acgggccaug caaagugccc gcucaaaugg ccguagauau gcagacccug acuccugucg     1680

gcagguuaau aacagcaaac ccagugauca cggagaguac cgaaaacucc aagaugaugc     1740

uggagcuaga ucccccguuc ggagacagcu acauuguuau cggcgugggg gagaaaaaga     1800

uaacucacca cuggcaucgg ucuggaucaa caauugguaa ggccuuugaa gcuaccgucc     1860

gcggcgccaa aagaauggca gugcucgggg acacagcuug ggauuucgga aguguaggcg     1920

gugcccugaa uagccuuggg aagggcaucc accagaucuu cggagcggcc uuuaaauccu     1980

uguucggcgg gaugucuugg uuuucgcaaa uucugaucgg aacucuccug auguggcuug     2040

gccugaacac caagaauggu agcauuuccu ugaugugucu cgcucuuggg ggaguguuaa     2100

uauuccuguc aacggcaguu agugccgacg ucggcugcuc uguggauuuu agcaaaugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6089
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6089
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucguc gggcugcucc uuacaacugc uauggcagcc gagguuacga      120

gaaggggauc cgcguacuau auguaccugg aucggaacga cgcuggugaa gccauuucuu      180

uccccaccac auugggcaug aauaagugcu auauacagau cauggauuua gggcacaugu      240

gugacgcaac uaugucauac gagugcccua ugcuggauga aggaguggag ccagacgaug      300

uagacuguug gugcaacacc acaaguaccu ggguggucua uggcacuugu caucacaaaa      360

agggugaagc ccgccgaagc cguagagcug ugacacuacc gucccauucu accaggaaac      420

uccaaacgcg gucgcagacu uggcuggagu cacgcgagua caccaagcac cuuauuagag      480

uugaaaauug gaucuuuagg aaccccgggu ucgcacuggc cgcugccgcg auugcauggu      540

ugcucggaag caguacaucc cagaaaguga ucuaucuggu caugauacug cucaucgccc      600

cugcuuacuc uauucggugc aucggcguga gcaaucgcga uuuuguagag ggcauguccg      660

ggggaacuug gguugacauu guguuagaac augguggcug ugucaccgug auggcccagg      720

auaagccaac aguggacauc gagcugguca ccacgacugu uucaaacaug gcagaggugc      780

gaaguuacug cuaugaagcu agcauaucug auauggccuc cgacucaaga ugucccacac      840

aaggggaggc guaccuugac aagcagagcg auacccagua uguaugcaaa aggacuuugg      900

uggaccgggg auggggcaau gguugugggc uguucggaaa gggcagucua gucacaugcg      960

ccaaauuugc augcucuaag aaaaugaccg gcaaguccau ucagccugaa aaccucgagu     1020

accguaucau gcugagcgug cacgggucgc aacacucugg aaugauuguu aaugauacgg     1080

gucaugaaac cgacgagaac cgcgcuaagg uggagaucac accaaauucc cccagagccg     1140

aagcuacucu uggcggguuc ggaucacugg gcuuggauug ugagccuagg accggucucg     1200

acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc cauaaggaau     1260

gguuccacga caucccacuc ccguggcacg ccggggcaga uacuggcaca ccccauugga     1320

acaauaaaga ggcccuugug gaauuuaagg acgcucacgc gaaacggcag accguaguug     1380

ugcugggaag ccaggagggc gcaguccaua ccgccuuggc uggggcccua gaggcagaaa     1440

uggauggagc caagggucga cuguccucug gccaccucaa augccgccug aagauggaca     1500

aacuuagacu gaagggggug agcuauucau uguguacugc ugccuucacg uuuacaaaga     1560

uuccugcaga gacccuccau ggaacuguca ccguggaagu ucaguacgcg ggcacagacg     1620

guccaugcaa agugcccgcu caaauggccg uagauaugca gacccugacu ccugucggga     1680

gguuaauaac agcaaaccca gugaucacgg agaguaccga aaacuccaag augaugcugg     1740

agcuagaucc cccguucggc gacagcuaca uuguuaucgg agugggcgag aaaaagauaa     1800

cucaccacug gcaucggucu gggucaacaa uuggaaaggc cuuugaagcu accguccgcg     1860

gugccaaaag aauggcagug cucggcgaca cagcuuggga uuucgggagu guaggaggcg     1920

cccugaauag ccuugguaag gggauccacc agaucuucgg cgcggccuuu aaauccuugu     1980

ucggaggcau gucuugguuu ucgcaaauuc ugaucgggac ucuccugaug uggcuuggac     2040

ugaacaccaa gaauggcagc auuuccuuga ugugucucgc ucuggguggg guguuaauau     2100

uccugucaac ggcaguuagu gccgacgucg gaugcucugu ggauuuuagc aaaugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6090
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6090
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgucggg cugcuccuua caacugccau ggcugcagag guuacgagaa      120

ggggauccgc cuacuauaug uaccuggauc ggaacgacgc gggugaagcu auuucuuucc      180

ccaccacauu gggcaugaau aagugcuaua uacagaucau ggauuuaggg cacaugugug      240

acgccacuau gucauacgag ugcccuaugc uggaugaagg aguggagcca gacgauguag      300

acuguuggug caacaccaca aguaccuggg uggucuaugg cacuugucau cacaaaaagg      360

gugaagcacg ccgaagccgu agagccguga cacuaccguc ccauucuacc aggaaacucc      420

aaacgcgguc gcagacuugg cuggagucac gcgaguacac caagcaccuu auuagaguug      480

aaaauuggau cuuuaggaac cccggguucg cucuggcagc cgcugccauu gcgugguugc      540

ucggaagcag uacaucccag aaagugaucu aucuggucau gauacugcuc aucgcacccg      600

ccuacucuau ucggugcauc ggcgugagca aucgcgauuu uguagagggc auguccgggg      660

gaacuugggu ugacauugug uuagaacaug guggcugugu caccgugaug gcucaggaua      720

agccaacagu ggacaucgag cuggucacca cgacuguuuc aaacauggcc gaggugcgaa      780

guuacugcua ugaagcaagc auaucugaua uggcuuccga cucaagaugu cccacacaag      840

gggaggccua ccuugacaag cagagcgaua cccaguaugu augcaaaagg acuuuggugg      900

accggggaug gggcaauggu ugugggcugu ucggaaaggg cagucuaguc acaugcgcga      960

aauuugccug cucuaagaaa augaccggca aguccauuca gccugaaaac cucgaguacc     1020

guaucaugcu gagcgugcac gggucgcaac acucuggaau gauuguuaau gauacggguc     1080

augaaaccga cgagaaccgc gcaaaggugg agaucacacc aaauuccccc agagcugaag     1140

ccacucuugg cggguucgga ucacugggcu uggauuguga gccuaggacc ggucucgacu     1200

uuagugaucu guauuacuua acaaugaaca auaaacacug gcugguccau aaggaauggu     1260

uccacgacau cccacucccg uggcacgcug gggccgauac uggcaccccu cauuggaaca     1320

auaaagaggc acuuguggaa uuuaaggacg cccacgcuaa acggcagaca guaguugugc     1380

ugggaagcca ggagggcgcg guccauaccg cauuggccgg ggcucuagag gccgaaaugg     1440

auggagcaaa gggucgacug uccucuggcc accucaaaug ccgccugaag auggacaaac     1500

uuagacugaa gggggugagc uauucauugu guacugccgc uuucacguuu acaaagauuc     1560

cugccgagac ccuccaugga acugucaccg uggaaguuca guacgcaggc acagacgguc     1620

caugcaaagu gcccgcgcaa auggcuguag auaugcagac ccugacuccu gucgggaggu     1680

uaauaacagc caacccagug aucacggaga guaccgaaaa cuccaagaug augcuggagc     1740

uagauccccc guucggcgac agcuacauug uuaucggagu gggcgagaaa aagauaacuc     1800

accacuggca ucggucuggg ucaacaauug gaaaggcauu ugaagccacc guccgcggug     1860

cuaaaagaau ggccgugcuc ggcgacacag caugggauuu cgggagugua ggaggcgcuc     1920

ugaauagccu ugguaagggg auccaccaga ucuucggcgc cgcguuuaaa uccuuguucg     1980

gaggcauguc uugguuuucg caaauucuga ucgggacucu ccugaugugg cuuggacuga     2040

acaccaagaa uggcagcauu uccuugaugu gucucgcccu ggguggggug uuaauauucc     2100

ugucaacggc uguuagugca gacgucggau gcucugugga uuuuagcaaa ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6091
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6091
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcagau gucggaugcu cuguggacuu uagcaaauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6092
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6092
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagccguu acgagaaggg      120

gauccgcgua cuauauguac cuggaccgga acgaugcugg ugaggccauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

caacuauguc auacgaaugc ccuaugcugg acgagggagu ggaaccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aggcccgccg aagccguaga gcugugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gaaucacgcg aguacaccaa gcaccuuauu agaguugaga      480

auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcuu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agaaggcaug uccgggggaa      660

cuuggguuga uauuguguua gagcauggug gcugugucac cgugauggcc caggacaagc      720

caacagugga uaucgaacug gucaccacga cuguuucaaa cauggcagag gugcgaaguu      780

acugcuauga ggcuagcaua ucugacaugg ccuccgauuc aagauguccc acacaagggg      840

aagcguaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgccaaau      960

uugcaugcuc uaagaaaaug accggcaagu ccauucagcc ugagaaccuc gaauaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

agaccgauga aaaccgcgcu aagguggaga ucacaccaaa uucccccaga gccgaggcua     1140

cucuuggcgg guucggauca cugggcuugg acugugaacc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gagugguucc     1260

acgauauccc acucccgugg cacgccgggg cagacacugg cacaccccau uggaacaaua     1320

aagaagcccu uguggaguuu aaggaugcuc acgcgaaacg gcagaccgua guugugcugg     1380

gaagccagga aggcgcaguc cauaccgccu uggcuggggc ccuagaggca gagauggacg     1440

gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca aagauuccug     1560

cagaaacccu ccauggaacu gucaccgugg agguucagua cgcgggcaca gacgguccau     1620

gcaaagugcc cgcucaaaug gccguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagcaaa cccagugauc acggaaagua ccgagaacuc caagaugaug cuggaacuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggccuuuga ggcuaccguc cgcggugcca     1860

aaagaauggc agugcucggc gauacagcuu gggacuucgg gaguguagga ggcgcccuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua auauuccugu     2100

caacggcagu uagugccgau gucggaugcu cuguggacuu uagcaaauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6093
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6093
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug gcagccgcgg      120

uuacgaggcg ggguuccgcu uacuauaugu accuggaucg caacgacgcc ggcgaggcaa      180

uuucuuuccc caccacauug gggaugaaua agugcuauau acagaucaug gauuuaggac      240

acauguguga cgccacuaug ucauacgaau gcccuaugcu ggaugagggc guggaaccag      300

acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu acuugucauc      360

acaaaaaggg ggaggcucga cguagcagaa gggcagugac acuaccgucc cauucuaccc      420

ggaaacucca aacgcgcucg cagacuuggc uggaaucaag agaguacacc aagcaccuua      480

uuaggguuga gaauuggauc uuucggaacc ccggauucgc ccuggcugcc gcggcaauug      540

ccugguugcu cggcagcagu acaucccaga aagugaucua ucuggucaug auacugcuca      600

ucgcuccugc cuacucuauu cgcugcaucg gcgugagcaa ucgagauuuu guagaaggga      660

uguccggagg uacuuggguu gacauugugu uagagcaugg cggguguguc accgugaugg      720

cacaggauaa gccaacagug gacaucgaac uggucaccac gacuguuuca aacauggcug      780

aggugagaag uuacugcuau gaggccagca uaucugauau ggcguccgac ucaagguguc      840

ccacacaagg agaagccuac cuugacaagc agagcgauac ccaguaugua ugcaaacgga      900

cuuuggugga ccguggcugg gguaaugggu guggacuguu cggcaagggc agucuaguca      960

caugcgcaaa auuugcuugc ucuaagaaaa ugaccgggaa guccauucag ccugagaacc     1020

ucgaauaccg caucaugcug agcgugcacg gaucgcaaca cucugguaug auuguuaaug     1080

auacgggcca ugagaccgac gaaaacagag ccaaggugga gaucacacca aauuccccca     1140

gggcugaggc cacucuuggg ggauucggcu cacuggguuu ggauugugaa ccucggaccg     1200

ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaaacacugg cugguccaua     1260

aggagugguu ccacgacauc ccacucccgu ggcacgcagg cgccgauacu ggaacacccc     1320

auuggaacaa uaaagaagcu cuuguggagu uuaaggacgc gcacgcaaaa cgacagaccg     1380

uaguugugcu gggcagccag gaaggggccg uccauaccgc uuuggccgga gcacuagagg     1440

ccgagaugga uggugcuaag ggccgccugu ccucugggca ccucaaaugc agacugaaga     1500

uggacaagcu gaggcugaaa ggagugagcu auucauugug uacugccgca uucacguuua     1560

caaagauucc ugcggaaacc cuccauggca cugucaccgu ggagguucag uacgcuggua     1620

cagacgggcc augcaaagug cccgcccaaa uggcaguaga uaugcagacc cugacuccug     1680

ucggccgguu aauaacagcc aacccaguga ucacggaaag uaccgagaac uccaagauga     1740

ugcuggaacu agaucccccg uucggagaca gcuacauugu uaucggcgug ggggagaaaa     1800

agauaacuca ccacuggcau cgcucuggau caacaauugg uaaggcuuuu gaggccaccg     1860

ucagaggcgc aaaaaggaug gcugugcucg gggacacagc cugggauuuc ggaaguguag     1920

gcggugcgcu gaauagccuu gggaagggca uccaccagau cuucggagcc gcuuuuaaau     1980

ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc cugauguggc     2040

uuggccugaa caccaagaau gguagcauuu ccuugaugug ucucgcacuu gggggagugu     2100

uaauauuccu gucaacggcc guuagugcug acgucggcug cucuguggau uuuagcaaau     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6094
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6094
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc caggaagcgg cgcucucacg acgugcucac cguccaguuc cuuauccugg      120

ggauguuguu aaugacagga gguguuacuc gacguggcuc agccuacuau auguaccugg      180

auagaaacga cgcuggggag gcaauuaguu uucccacgac ccuaggaaug aauaaaugcu      240

auauacaaau cauggaucuc ggccauaugu gugacgccac aaugagcuac gaaugcccua      300

ugcuggauga ggguguggaa ccagacgaug uagacuguug gugcaacacu accuccacau      360

ggguggucua ugggaccugu caccauaaga aaggagaggc gaggcggucu cgcagagcug      420

ugacucuucc gucgcacuca acaaggaagc ugcagacccg gagccagacg ugguuggaaa      480

gucgcgagua cacuaaacau cucauucgag uugagaauug gaucuucaga aaccccggcu      540

uugcccuggc agccgcugca auugccuggc ugcucggcuc cucuaccagc cagaagguga      600

ucuauuuagu caugauacug cuuaucgcuc cugccuacuc cauuaggugc aucggggugu      660

caaaucggga uuucguagaa ggaaugagug guggcacaug gguugacauu guguuggagc      720

acgggggaug ugucacugug auggcgcaag auaagccaac cguggacauc gaacugguca      780

caaccacggu uagcaacaug gcagaggugc guucuuacug cuaugaggcc uccauaucag      840

auauggcuag cgacagucgc ugucccacuc agggcgaagc cuaccuagac aaacagucug      900

auacacagua uguaugcaag agaacccucg uggacagggg uugggggaau ggauguggcc      960

uguuuggcaa agggucccuu gucacuugcg caaaguucgc uugcagcaaa aagaugacag     1020

gaaagucgau ucaaccugag aaccuggaau accggaucau guugucugug cacgguuccc     1080

agcauucagg caugauuguu aaugauaccg ggcacgagac ggacgaaaac cgagccaaag     1140

uggagaucac cccaaauagu ccccgcgcgg aggccacacu cggaggcuuu gguagccugg     1200

gguuagauug ugaaccuaga acuggccugg acuucuccga ucucuauuac cuuaccauga     1260

acaauaagca uuggcugguc cacaaagagu gguuucacga caucccauug ccguggcaug     1320

caggagcuga uacaggcacu ccccacugga acaauaagga agcccuagug gaguucaaag     1380

acgcucaugc caagaggcag accguaguug ugcugggguc ucaggaagga gcaguccaca     1440

cagcccucgc uggugcgcug gaggcagaga uggauggcgc caaagggcgg cuuagcucag     1500

gacaucugaa gugccgcuug aagauggaca aacucagacu gaagggcgug aguuauuccu     1560

uauguaccgc ugccuuuacu uucacgaaaa uuccugcaga aacacugcac gguaccguca     1620

cuguggaggu ucaauacgcc gggaccgacg gcccaugcaa ggugcccgcu cagauggccg     1680

uagauaugca gacacuaacc ccugucggaa ggcucauaac ugcaaaccca gugaucacag     1740

aaagcacgga gaacucuaaa augaugcugg aacuugaucc cccguuuggc gacucauaca     1800

uuguuaucgg ggugggagag aagaagauaa cccaccauug gcaccguagu gguagcacua     1860

uuggcaaagc guucgaggcu acaguccgag gggccaagcg gauggcagug uugggagaca     1920

ccgccuggga uuucggcucc guaggugggg cucucaauuc ucugggcaaa ggaauccauc     1980

aaaucuuugg cgccgcauuc aagucgcugu uugggggaau gagcugguuc ucccagauuc     2040

uuaucggcac acuguugaug uggcucgguc ugaacacuaa aaauggguca auuaguuuaa     2100

ugugucuggc ucuaggaggc gugcucauau uucugucuac cgccguuagc gcggacgucg     2160

guugcuccgu ggauuucuca aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6095
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6095
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc caggaagcgg cgcucucacg acgugcucac cguccaguuc cuuauccugg      120

ggauguuguu aaugacagga gccguuacuc gacgugguuc agcuuacuau auguaccugg      180

auagaaacga cgcaggcgag gccauuaguu uucccacgac ccuagggaug aauaaaugcu      240

auauacaaau cauggaucuc ggacauaugu gugacgcgac aaugagcuac gaaugcccua      300

ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacu accuccacau      360

ggguggucua ugguaccugu caccauaaga aaggggaggc uaggcggucu cgcagagccg      420

ugacucuucc gucgcacuca acaaggaagc ugcagacccg gagccagacg ugguuggaaa      480

gucgcgagua cacuaaacau cucauucgag uugagaauug gaucuucaga aaccccggau      540

uugcacuggc cgcugcagcc auugcuuggc ugcucggcuc cucuaccagc cagaagguga      600

ucuauuuagu caugauacug cuuaucgccc cugcguacuc cauuaggugc aucggcgugu      660

caaaucggga uuucguagaa gggaugagug gagguacaug gguugacauu guguuggagc      720

acggcgggug ugucacugug auggcacaag auaagccaac cguggacauc gaacugguca      780

caaccacggu uagcaacaug gccgaggugc guucuuacug cuaugaggcu uccauaucag      840

auauggccag cgacagucgc ugucccacuc agggagaagc auaccuagac aaacagucug      900

auacacagua uguaugcaag agaacccucg uggacagggg cugggguaau ggguguggac      960

uguuuggcaa aggcucccuu gucacuugcg cuaaguucgc cugcagcaaa aagaugacag     1020

ggaagucgau ucaaccugag aaccuggaau accggaucau guugucugug cacggauccc     1080

agcauucagg uaugauuguu aaugauaccg gccacgagac ggacgaaaac cgagcgaaag     1140

uggagaucac cccaaauagu ccccgcgccg aggcaacacu cgggggauuu ggcagccugg     1200

guuuagauug ugaaccuaga acugggcugg acuucuccga ucucuauuac cuuaccauga     1260

acaauaagca uuggcugguc cacaaagagu gguuucacga caucccauug ccguggcaug     1320

cuggcgccga uacaggaacu ccccacugga acaauaagga agcucuagug gaguucaaag     1380

acgcccaugc aaagaggcag accguaguug ugcugggcuc ucaggaaggg gccguccaca     1440

cagcucucgc gggagcacug gaggccgaga uggauggugc uaaaggccgg cuuagcucag     1500

ggcaucugaa gugccgcuug aagauggaca aacucagacu gaagggagug aguuauuccu     1560

uauguaccgc cgcauuuacu uucacgaaaa uuccugccga aacacugcac ggcaccguca     1620

cuguggaggu ucaauacgcu gguaccgacg ggccaugcaa ggugcccgcc cagauggcag     1680

uagauaugca gacacuaacc ccugucggca ggcucauaac ugcgaaccca gugaucacag     1740

aaagcacgga gaacucuaaa augaugcugg aacuugaucc cccguuugga gacucauaca     1800

uuguuaucgg cgugggggag aagaagauaa cccaccauug gcaccguagu ggaagcacua     1860

uugguaaggc uuucgaggcc acaguccggg gcgcaaaacg aauggccgug uugggggaca     1920

ccgcuuggga uuucggaucc guaggcggug cccucaauuc ucuggggaaa ggcauccauc     1980

aaaucuuugg agcagcuuuc aagucgcugu uuggcgggau gagcugguuc ucccagauuc     2040

uuaucggaac acuguugaug uggcucggcc ugaacacuaa aaaugguuca auuaguuuaa     2100

ugugucuggc ccuaggggga gugcucauau uucugucuac cgcgguuagc gccgacgucg     2160

gcugcuccgu ggauuucuca aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6096
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6096
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacauag      720

uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca guggacauug      780

agcuggucac cacgacuguu ucaaauaugg cugaagugcg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu ggaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuugugg     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguaguugu gcugggcagc caggaggggg     1440

ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggaa acccuccaug     1620

gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag acccugacuc cugucggcag guuaauuaca gccaacccag     1740

ugaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggacac agccugggau uucggaagug uaggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag ugcugauuuu ccugucaacg gccguuagug     2160

cugacgucgg cugcugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6097
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6097
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uuaacgccag aaaggagaaa aagaggcggg gcgcugacac cagcgugggg auagucggac      120

ugcuccuuac aacugcaaug gccgcggaag uuacgcgccg agguuccgcu uacuauaugu      180

accuggaucg uaaugacgcc ggcgaggcaa ucucuuuccc caccacauug gggaugaaca      240

aaugcuauau ucagaucaug gauuuaggac acauguguga cgccacuaug ucauacgaau      300

gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc aauaccacaa      360

guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggcuaga aggagccggc      420

gcgcagugac acuaccgucc cauucuacca gaaagcucca aacgaggucg cagacuuggc      480

uggagucacg ggaauacacc aagcaccuua uucgcguuga gaacuggauc uuucgaaauc      540

ccggauucgc ccuggcugcc gcggcaauag ccugguugcu cggcagcagu acaucccaga      600

aagugaucua ucuggucaug auucugcuca ucgcuccugc cuacucuauu agaugcaucg      660

gcgugagcaa cagggauuuu guagaaggga uguccggagg uacuuggguu gacauagugu      720

uagagcaugg cggguguguc accgugaugg cacaggauaa gccaacagug gacauugagc      780

uggucaccac gacuguuuca aauauggcug aagugcggag uuacugcuau gaggccagca      840

ucucugauau ggcguccgac ucacguuguc ccacacaagg agaagccuac cuugacaaac      900

agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg gguaacgggu      960

guggacuguu cggcaaaggc agucuaguca caugcgcaaa guuugcuugc ucuaagaaaa     1020

ugaccgggaa guccauucag ccugagaauc ucgaauacag gaucaugcug agcgugcacg     1080

gaucgcaaca cucugguaug aucguuaacg auacgggcca ugagaccgac gagaaucggg     1140

ccaaagugga aauuacacca aacucacccc gagcugaggc cacucuuggg ggauucggcu     1200

cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug uauuacuuaa     1260

caaugaauaa caagcacugg cugguccaua aagagugguu ccacgacaua ccacucccgu     1320

ggcacgcagg cgccgauacu ggaacacccc auuggaauaa caaggaagcu cuuguggagu     1380

uuaaagacgc gcacgcaaag agacagaccg uaguugugcu gggcagccag gagggggccg     1440

uccauaccgc uuuggccgga gcacuagaag ccgagaugga uggugcuaag ggcaggcugu     1500

ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag ggagugagcu     1560

auucauugug uacugccgca uucacguuua caaaaauccc ugcggaaacc cuccauggca     1620

cugucaccgu ggagguucag uacgcuggua cagacgggcc augcaaggug cccgcccaaa     1680

uggcaguaga uaugcagacc cugacuccug ucggcagauu aauuacagcc aacccaguga     1740

ucacggaaag uaccgaaaau uccaagauga ugcuggagcu agaucccccg uucggagaca     1800

gcuacauagu uauuggcgug ggggagaaaa agaucacuca ccacuggcau aggucuggau     1860

caacaaucgg uaaggcuuuu gaggccaccg uccguggcgc aaaacggaug gcugugcucg     1920

gggacacagc cugggauuuc ggaaguguag gcggugcgcu gaacagccuu gggaaaggca     1980

uucaccagau cuucggagcc gcuuuuaagu ccuuguucgg cgggaugucu ugguuuucgc     2040

aaauucugau aggaacucuc cugauguggc uuggccugaa uaccaagaac gguagcaucu     2100

ccuugaugug ucucgcauua gggggagugc ugauuuuccu gucaacggcc guuagugcug     2160

acgucggcug cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  6098
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6098
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaaaaag aggcggggcg cugacaccag cguggggauu gucggacugc      120

uccuuacaac ugcaauggcc gcggaaguua cgcgccgagg uuccgcuuac uauauguacc      180

uggaucguaa ugacgccggc gaggcaauau cuuuccccac cacauugggg augaacaaau      240

gcuauaucca gauuauggau uuaggacaca ugugugacgc cacuauguca uacgaaugcc      300

cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau accacaagua      360

ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuagaagg agccggcgcg      420

cagugacacu accgucccau ucuaccagaa agcuccaaac gaggucgcag acuuggcugg      480

agucacggga auacaccaag caccuuaucc gcguugagaa cuggauuuuu cgaaaucccg      540

gauucgcccu ggcugccgcg gcaaucgccu gguugcucgg cagcaguaca ucccagaaag      600

ugauauaucu ggucaugauc cugcucauug cuccugccua cucuaucaga ugcauuggcg      660

ugagcaacag ggauuuugua gaagggaugu ccggagguac uuggguugac aucguguuag      720

agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac auagagcugg      780

ucaccacgac uguuucaaau auggcugaag ugcggaguua cugcuaugag gccagcauuu      840

cugauauggc guccgacuca cguuguccca cacaaggaga agccuaccuu gacaaacaga      900

gcgauaccca guauguaugc aagcgcacuu ugguggacag aggcuggggu aacgggugug      960

gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu aagaaaauga     1020

ccgggaaguc cauccagccu gagaaucucg aauacaggau uaugcugagc gugcacggau     1080

cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag aaucgggcca     1140

aaguggaaau cacaccaaac ucaccccgag cugaggccac ucuuggggga uucggcuccc     1200

uggguuugga uugugaaccu cgcaccgggc ucgacuuuag ugaucuguau uacuuaacaa     1260

ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauucca cucccguggc     1320

acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu guggaguuua     1380

aagacgcgca cgcaaagaga cagaccguag uugugcuggg cagccaggag ggggccgucc     1440

auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc aggcuguccu     1500

cugggcaccu caaaugccgg cugaagaugg acaaacuucg ccugaaggga gugagcuauu     1560

cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc cauggcacug     1620

ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc gcccaaaugg     1680

caguagauau gcagacccug acuccugucg gcagauuaau cacagccaac ccagugauua     1740

cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc ggagacagcu     1800

acaucguuau aggcgugggg gagaaaaaga uuacucacca cuggcauagg ucuggaucaa     1860

caaucgguaa ggcuuuugag gccaccgucc guggcgcaaa acggauggcu gugcucgggg     1920

acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg aaaggcaucc     1980

accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg uuuucgcaaa     2040

uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu agcauauccu     2100

ugaugugucu cgcauuaggg ggagugcuga ucuuccuguc aacggccguu agugcugacg     2160

ucggcugcug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  6099
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6099
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaaaaagagg cggggcgcug acaccagcgu ggggaucguc ggacugcucc      120

uuacaacugc aauggccgcg gaaguuacgc gccgagguuc cgcuuacuau auguaccugg      180

aucguaauga cgccggcgag gcaauuucuu uccccaccac auuggggaug aacaaaugcu      240

auauacagau cauggauuua ggacacaugu gugacgccac uaugucauac gaaugcccua      300

ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaauacc acaaguaccu      360

ggguggucua ugguacuugu caucacaaga aaggggaggc uagaaggagc cggcgcgcag      420

ugacacuacc gucccauucu accagaaagc uccaaacgag gucgcagacu uggcuggagu      480

cacgggaaua caccaagcac cuuauucgcg uugagaacug gaucuuucga aaucccggau      540

ucgcccuggc ugccgcggca auugccuggu ugcucggcag caguacaucc cagaaaguga      600

ucuaucuggu caugauacug cucaucgcuc cugccuacuc uauuagaugc aucggcguga      660

gcaacaggga uuuuguagaa gggauguccg gagguacuug gguugacauu guguuagagc      720

auggcgggug ugucaccgug auggcacagg auaagccaac aguggacauc gagcugguca      780

ccacgacugu uucaaauaug gcugaagugc ggaguuacug cuaugaggcc agcauaucug      840

auauggcguc cgacucacgu ugucccacac aaggagaagc cuaccuugac aaacagagcg      900

auacccagua uguaugcaag cgcacuuugg uggacagagg cugggguaac ggguguggac      960

uguucggcaa aggcagucua gucacaugcg caaaguuugc uugcucuaag aaaaugaccg     1020

ggaaguccau ucagccugag aaucucgaau acaggaucau gcugagcgug cacggaucgc     1080

aacacucugg uaugauuguu aacgauacgg gccaugagac cgacgagaau cgggccaaag     1140

uggaaaucac accaaacuca ccccgagcug aggccacucu ugggggauuc ggcucccugg     1200

guuuggauug ugaaccucgc accgggcucg acuuuaguga ucuguauuac uuaacaauga     1260

auaacaagca cuggcugguc cauaaagagu gguuccacga caucccacuc ccguggcacg     1320

caggcgccga uacuggaaca ccccauugga auaacaagga agcucuugug gaguuuaaag     1380

acgcgcacgc aaagagacag accguaguug ugcugggcag ccaggagggg gccguccaua     1440

ccgcuuuggc cggagcacua gaagccgaga uggauggugc uaagggcagg cuguccucug     1500

ggcaccucaa augccggcug aagauggaca aacuucgccu gaagggagug agcuauucau     1560

uguguacugc cgcauucacg uuuacaaaaa uuccugcgga aacccuccau ggcacuguca     1620

ccguggaggu ucaguacgcu gguacagacg ggccaugcaa ggugcccgcc caaauggcag     1680

uagauaugca gacccugacu ccugucggca gauuaauaac agccaaccca gugaucacgg     1740

aaaguaccga aaauuccaag augaugcugg agcuagaucc cccguucgga gacagcuaca     1800

uuguuaucgg cgugggggag aaaaagauaa cucaccacug gcauaggucu ggaucaacaa     1860

uugguaaggc uuuugaggcc accguccgug gcgcaaaacg gauggcugug cucggggaca     1920

cagccuggga uuucggaagu guaggcggug cgcugaacag ccuugggaaa ggcauccacc     1980

agaucuucgg agccgcuuuu aaguccuugu ucggcgggau gucuugguuu ucgcaaauuc     2040

ugaucggaac ucuccugaug uggcuuggcc ugaauaccaa gaacgguagc auuuccuuga     2100

ugugucucgc auuaggggga gugcugauau uccugucaac ggccguuagu gcugacgucg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6100
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6100
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa aaagaggcgg ggcgcugaca ccagcguggg gaucgucgga cugcuccuua      120

caacugcaau ggccgcggaa guuacgcgcc gagguuccgc uuacuauaug uaccuggauc      180

guaacgacgc cggcgaggca auuucuuucc ccaccacauu ggggaugaau aaaugcuaua      240

uacagaucau ggauuuagga cacaugugug acgccacuau gucauacgaa ugcccuaugc      300

uggaugaggg cguggaacca gacgauguag acuguuggug caacaccaca aguaccuggg      360

uggucuaugg uacuugucau cacaagaaag gggaggcuag aaggagccgg cgcgcaguga      420

cacuaccguc ccauucuacc agaaagcucc aaacgagguc gcagacuugg cuggagucac      480

gggaauacac caagcaccuu auucgcguug agaauuggau cuuucgaaac cccggauucg      540

cccuggcugc cgcggcaauu gccugguugc ucggcagcag uacaucccag aaagugaucu      600

aucuggucau gauacugcuc aucgcuccug ccuacucuau uagaugcauc ggcgugagca      660

auagggauuu uguagaaggg auguccggag guacuugggu ugacauugug uuagagcaug      720

gcgggugugu caccgugaug gcacaggaua agccaacagu ggacaucgag cuggucacca      780

cgacuguuuc aaacauggcu gaagugcgga guuacugcua ugaggccagc auaucugaua      840

uggcguccga cucacguugu cccacacaag gagaagccua ccuugacaaa cagagcgaua      900

cccaguaugu augcaagcgc acuuuggugg acagaggcug ggguaauggg uguggacugu      960

ucggcaaagg cagucuaguc acaugcgcaa aguuugcuug cucuaagaaa augaccggga     1020

aguccauuca gccugagaac cucgaauaca ggaucaugcu gagcgugcac ggaucgcaac     1080

acucugguau gauuguuaau gauacgggcc augagaccga cgagaaccgg gccaaagugg     1140

aaaucacacc aaauucaccc cgagcugagg ccacucuugg gggauucggc ucccuggguu     1200

uggauuguga accucgcacc gggcucgacu uuagugaucu guauuacuua acaaugaaca     1260

auaagcacug gcugguccau aaagaguggu uccacgacau cccacucccg uggcacgcag     1320

gcgccgauac uggaacaccc cauuggaaca auaaggaagc ucuuguggag uuuaaagacg     1380

cgcacgcaaa gagacagacc guaguugugc ugggcagcca ggagggggcc guccauaccg     1440

cuuuggccgg agcacuagaa gccgagaugg auggugcuaa gggcaggcug uccucugggc     1500

accucaaaug ccggcugaag auggacaaac uucgccugaa gggagugagc uauucauugu     1560

guacugccgc auucacguuu acaaaaauuc cugcggaaac ccuccauggc acugucaccg     1620

uggagguuca guacgcuggu acagacgggc caugcaaggu gcccgcccaa auggcaguag     1680

auaugcagac ccugacuccu gucggcagau uaauaacagc caacccagug aucacggaaa     1740

guaccgagaa cuccaagaug augcuggagc uagauccccc guucggagac agcuacauug     1800

uuaucggcgu gggggagaaa aagauaacuc accacuggca uaggucugga ucaacaauug     1860

guaaggcuuu ugaagccacc guccguggcg caaaacggau ggcugugcuc ggggacacag     1920

ccugggauuu cggaagugua ggcggugcgc ugaauagccu ugggaaaggc auccaccaga     1980

ucuucggagc cgcuuuuaag uccuuguucg gcgggauguc uugguuuucg caaauucuga     2040

ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu uccuugaugu     2100

gucucgcauu agggggagug cugauauucc ugucaacggc cguuagugcu gacgucggcu     2160

gcugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6101
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6101
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaaaaa gaggcggggc gccgacacca gcguggggau cgucggacug cuccuuacaa      120

cugcuauggc agccgaaguu acgcgccgag guuccgcgua cuauauguac cuggaucgua      180

acgacgcugg cgaggccauu ucuuucccca ccacauuggg gaugaauaaa ugcuauauac      240

agaucaugga uuuaggacac augugugacg caacuauguc auacgaaugc ccuaugcugg      300

augagggcgu ggaaccagac gauguagacu guuggugcaa caccacaagu accugggugg      360

ucuaugguac uugucaucac aagaaagggg aggccagaag gagccggcgc gcugugacac      420

uaccguccca uucuaccaga aagcuccaaa cgaggucgca gacuuggcug gagucacggg      480

aauacaccaa gcaccuuauu cgcguugaga auuggaucuu ucgaaacccc ggauucgcac      540

uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaaa gugaucuauc      600

uggucaugau acugcucauc gccccugcuu acucuauuag augcaucggc gugagcaaua      660

gggauuuugu agaagggaug uccggaggua cuuggguuga cauuguguua gagcauggcg      720

ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug gucaccacga      780

cuguuucaaa cauggcagaa gugcggaguu acugcuauga ggcuagcaua ucugauaugg      840

ccuccgacuc acguuguccc acacaaggag aagcguaccu ugacaaacag agcgauaccc      900

aguauguaug caagcgcacu uugguggaca gaggcugggg uaaugggugu ggacuguucg      960

gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaagaaaaug accgggaagu     1020

ccauucagcc ugagaaccuc gaauacagga ucaugcugag cgugcacgga ucgcaacacu     1080

cugguaugau uguuaaugau acgggccaug agaccgacga gaaccgggcu aaaguggaaa     1140

ucacaccaaa uucaccccga gccgaggcua cucuuggggg auucggcucc cuggguuugg     1200

auugugaacc ucgcaccggg cucgacuuua gugaucugua uuacuuaaca augaacaaua     1260

agcacuggcu gguccauaaa gagugguucc acgacauccc acucccgugg cacgccggcg     1320

cagauacugg aacaccccau uggaacaaua aggaagcccu uguggaguuu aaagacgcuc     1380

acgcgaagag acagaccgua guugugcugg gcagccagga gggggcaguc cauaccgccu     1440

uggcuggagc ccuagaagca gagauggaug gugccaaggg caggcugucc ucugggcacc     1500

ucaaaugccg gcugaagaug gacaaacuuc gccugaaggg agugagcuau ucauugugua     1560

cugcugccuu cacguuuaca aaaauuccug cagaaacccu ccauggcacu gucaccgugg     1620

agguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug gccguagaua     1680

ugcagacccu gacuccuguc ggcagauuaa uaacagcaaa cccagugauc acggaaagua     1740

ccgagaacuc caagaugaug cuggagcuag aucccccguu cggagacagc uacauuguua     1800

ucggcguggg ggagaaaaag auaacucacc acuggcauag gucuggauca acaauuggua     1860

aagccuuuga agcuaccguc cguggcgcca agcggauggc agugcucggg gacacagcuu     1920

gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc caccagaucu     1980

ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa auucugaucg     2040

gaacucuccu gauguggcuu ggccugaaca ccaagaaugg uagcauuucc uugauguguc     2100

ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac gucggcugcu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6102
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6102
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaaaaagag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc cuuacaacug      120

cuauggcagc cgaaguuacg cggcgcgguu ccgcguacua uauguaccug gaucgaaacg      180

acgcuggcga ggccauuucu uuccccacca cauuggggau gaauaaaugc uauauacaga      240

ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgaaugcccu augcuggaug      300

agggcgugga accagacgau guagacuguu ggugcaacac cacaaguacc uggguggucu      360

augguacuug ucaucacaag aaaggggagg cccguagaag caggcgggcu gugacacuac      420

cgucccauuc uacccgcaag cuccaaacga gaucgcagac uuggcuggag ucaagggaau      480

acaccaagca ccuuauucgg guugagaauu ggaucuuucg caaccccgga uucgcacugg      540

ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug aucuaucugg      600

ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug agcaauagag      660

auuuuguaga agggaugucc ggagguacuu ggguugacau uguguuagag cauggcgggu      720

gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc accacgacug      780

uuucaaacau ggcagaagug aggaguuacu gcuaugaggc uagcauaucu gauauggccu      840

ccgacucacg gugucccaca caaggagaag cguaccuuga caaacagagc gauacccagu      900

auguaugcaa gcguacuuug guggaccgcg gcugggguaa ugggugugga cuguucggca      960

aaggcagucu agucacaugc gccaaguuug caugcucuaa gaaaaugacc gggaagucca     1020

uucagccuga gaaccucgaa uacagaauca ugcugagcgu gcacggaucg caacacucug     1080

guaugauugu uaaugauacg ggccaugaga ccgacgagaa cagggcuaaa guggaaauca     1140

caccaaauuc accccgggcc gaggcuacuc uugggggauu cggcucccug gguuuggauu     1200

gugaaccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug aacaauaagc     1260

acuggcuggu ccauaaagag ugguuccacg acaucccacu cccguggcac gccggcgcag     1320

auacuggaac accccauugg aacaauaagg aagcccuugu ggaguuuaaa gacgcucacg     1380

cgaagcgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau accgccuugg     1440

cuggagcccu agaagcagag auggauggug ccaagggcag acuguccucu gggcaccuca     1500

aaugcaggcu gaagauggac aaacuucggc ugaagggagu gagcuauuca uuguguacug     1560

cugccuucac guuuacaaaa auuccugcag aaacccucca uggcacuguc accguggagg     1620

uucaguacgc ggguacagac gggccaugca aggugcccgc ucaaauggcc guagauaugc     1680

agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg gaaaguaccg     1740

agaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac auuguuaucg     1800

gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca auugguaaag     1860

ccuuugaagc uaccgucagg ggcgccaagc guauggcagu gcucggggac acagcuuggg     1920

auuucggaag uguaggcggu gcccugaaua gccuugggaa aggcauccac cagaucuucg     1980

gagcggccuu uaaguccuug uucggcggga ugucuugguu uucgcaaauu cugaucggaa     2040

cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug augugucucg     2100

cuuuaggggg agugcugaua uuccugucaa cggcaguuag ugccgacguc ggcugcugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6103
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6103
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagaag gggcgccgac accagcgugg ggaucgucgg acugcuccuu acaacugcua      120

uggcagccga aguuacgcgg cgcgguuccg cguacuauau guaccuggau cgaaacgacg      180

cuggcgaggc cauuucuuuc cccaccacau uggggaugaa uaagugcuau auacagauca      240

uggauuuagg acacaugugu gacgcaacua ugucauacga augcccuaug cuggaugagg      300

gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg guggucuaug      360

guacuuguca ucacaaaaag ggggaggccc guagaagcag gcgggcugug acacuaccgu      420

cccauucuac ccgcaaacuc caaacgagau cgcagacuug gcuggaguca agggaauaca      480

ccaagcaccu uauucggguu gagaauugga ucuuucgcaa ccccggauuc gcacuggccg      540

cugccgcgau ugcaugguug cucggcagca guacauccca gaaggugauc uaucugguca      600

ugauacugcu caucgccccu gcuuacucua uucgaugcau cggcgugagc aauagagauu      660

uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau ggcgggugug      720

ucaccgugau ggcccaggau aaaccaacag uggacaucga gcuggucacc acgacuguuu      780

caaacauggc agaagugagg aguuacugcu augaggcuag cauaucugau auggccuccg      840

acucacggug ucccacacaa ggagaagcgu accuugacaa gcagagcgau acccaguaug      900

uaugcaaacg uacuuuggug gaccgcggcu gggguaaugg guguggacug uucggcaagg      960

gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa gaugaccggg aaauccauuc     1020

agccugagaa ccucgaauac agaaucaugc ugagcgugca cggaucgcaa cacucuggua     1080

ugauuguuaa ugauacgggc caugagaccg acgagaacag ggcuaaggug gaaaucacac     1140

caaauucacc ccgggccgag gcuacucuug ggggauucgg cucccugggu uuggauugug     1200

aaccucgaac cgggcucgac uuuagugauc uguauuacuu aacaaugaac aauaaacacu     1260

ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgcc ggcgcagaua     1320

cuggaacacc ccauuggaac aauaaagaag cccuugugga guuuaaggac gcucacgcga     1380

aacgccagac cguaguugug cugggcagcc aggagggggc aguccauacc gccuuggcug     1440

gagcccuaga agcagagaug gauggugcca agggcagacu guccucuggg caccucaagu     1500

gcaggcugaa aauggacaag cugcggcuga aaggagugag cuauucauug uguacugcug     1560

ccuucacguu uacaaagauu ccugcagaaa cccuccaugg cacugucacc guggagguuc     1620

aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua gauaugcaga     1680

cccugacucc ugucggccgc uuaauaacag caaacccagu gaucacggaa aguaccgaga     1740

acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu guuaucggcg     1800

ugggggaaaa gaaaauaacu caccacuggc auagaucugg aucaacaauu gguaaggccu     1860

uugaggcuac cgucaggggc gccaaacgua uggcagugcu cggggacaca gcuugggauu     1920

ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag aucuucggag     1980

cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug aucggaacuc     2040

uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug ugucucgcuc     2100

uugggggagu guuaauauuc cugucaacgg caguuagugc cgacgucggc ugcugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6104
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6104
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagaagggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca acugcuaugg      120

cagccgaggu uacgcggcgc gguuccgcgu acuauaugua ccuggaucga aacgacgcug      180

gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua cagaucaugg      240

auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug gaugaaggcg      300

uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug gucuauggua      360

cuugucauca caaaaagggg gaagcccgua gaagcaggcg ggcugugaca cuaccguccc      420

auucuacccg caaacuccaa acgagaucgc agacuuggcu ggagucaagg gaguacacca      480

agcaccuuau ucggguugaa aauuggaucu uucgcaaccc cggauucgca cuggccgcug      540

ccgcgauugc augguugcuc ggcagcagua caucccagaa ggugaucuau cuggucauga      600

uacugcucau cgccccugcu uacucuauuc gaugcaucgg cgugagcaau agagauuuug      660

uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc ggguguguca      720

ccgugauggc ccaggauaaa ccaacagugg acaucgagcu ggucaccacg acuguuucaa      780

acauggcaga ggugaggagu uacugcuaug aagcuagcau aucugauaug gccuccgacu      840

cacggugucc cacacaagga gaggcguacc uugacaagca gagcgauacc caguauguau      900

gcaaacguac uuugguggac cgcggcuggg guaaugggug uggacuguuc ggcaagggca      960

gucuagucac augcgccaaa uuugcaugcu cuaagaagau gaccgggaaa uccauucagc     1020

cugaaaaccu cgaguacaga aucaugcuga gcgugcacgg aucgcaacac ucugguauga     1080

uuguuaauga uacgggccau gaaaccgacg agaacagggc uaagguggag aucacaccaa     1140

auucaccccg ggccgaagcu acucuugggg gauucggcuc ccuggguuug gauugugagc     1200

cucgaaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau aaacacuggc     1260

ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc gcagauacug     1320

gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu cacgcgaaac     1380

gccagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc uuggcuggag     1440

cccuagaggc agaaauggau ggugccaagg gcagacuguc cucugggcac cucaagugca     1500

ggcugaaaau ggacaagcug cggcugaaag gagugagcua uucauugugu acugcugccu     1560

ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug gaaguucagu     1620

acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau augcagaccc     1680

ugacuccugu cggccgcuua auaacagcaa acccagugau cacggagagu accgaaaacu     1740

ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu aucggcgugg     1800

gggagaagaa aauaacucac cacuggcaua gaucuggauc aacaauuggu aaggccuuug     1860

aagcuaccgu caggggcgcc aaacguaugg cagugcucgg ggacacagcu ugggauuucg     1920

gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc uucggagcgg     1980

ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc ggaacucucc     2040

ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu cucgcucuug     2100

ggggaguguu aauauuccug ucaacggcag uuagugccga cgucggcugc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6105
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6105
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gaaggggcgc cgacaccagc guggggaucg ucggacugcu ccuuacaacu gcuauggcag      120

ccgagguuac gcggcgcggu uccgcguacu auauguaccu ggaucgaaac gacgcuggcg      180

aagccauuuc uuuccccacc acauugggga ugaauaaaug cuauauacag aucauggauu      240

uaggacacau gugugacgca acuaugucau acgagugccc uaugcuggau gaaggcgugg      300

agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc uaugguacuu      360

gucaucacaa gaaaggggaa gcccguagaa gcaggcgggc ugugacacua ccgucccauu      420

cuacccgcaa gcuccaaacg agaucgcaga cuuggcugga gucaagggag uacaccaaac      480

accuuauucg gguugaaaau uggaucuuuc gcaaccccgg auucgcacug gccgcugccg      540

cgauugcaug guugcucggc agcaguacau cccagaaggu gaucuaucug gucaugauac      600

ugcucaucgc cccugcuuac ucuauucgau gcaucggcgu gagcaauaga gauuuuguag      660

aggggauguc cggagguacu uggguugaca uuguguuaga acauggcggg ugugucaccg      720

ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu guuucaaaca      780

uggcagaggu gaggaguuac ugcuaugaag cuagcauauc ugauauggcc uccgacucac      840

ggugucccac acaaggagag gcguaccuug acaaacagag cgauacccag uauguaugca      900

agcguacuuu gguggaccgc ggcuggggua augggugugg acuguucggc aaaggcaguc      960

uagucacaug cgccaaguuu gcaugcucua aaaagaugac cgggaagucc auucagccug     1020

aaaaccucga guacagaauc augcugagcg ugcacggauc gcaacacucu gguaugauug     1080

uuaaugauac gggccaugaa accgacgaga acagggcuaa aguggagauc acaccaaauu     1140

caccccgggc cgaagcuacu cuugggggau ucggcucccu ggguuuggau ugugagccuc     1200

gaaccgggcu cgacuuuagu gaucuguauu acuuaacaau gaacaauaag cacuggcugg     1260

uccauaaaga augguuccac gacaucccac ucccguggca cgccggcgca gauacuggaa     1320

caccccauug gaacaauaag gaggcccuug uggaauuuaa agacgcucac gcgaagcgcc     1380

agaccguagu ugugcugggc agccaggagg gggcagucca uaccgccuug gcuggagccc     1440

uagaggcaga aauggauggu gccaaaggca gacuguccuc ugggcaccuc aagugcaggc     1500

ugaagaugga caaacuucgg cugaagggag ugagcuauuc auuguguacu gcugccuuca     1560

cguuuacaaa aauuccugca gagacccucc auggcacugu caccguggaa guucaguacg     1620

cggguacaga cgggccaugc aaggugcccg cucaaauggc cguagauaug cagacccuga     1680

cuccugucgg ccgcuuaaua acagcaaacc cagugaucac ggagaguacc gaaaacucca     1740

aaaugaugcu ggagcuagau cccccguucg gagacagcua cauuguuauc ggcguggggg     1800

agaagaagau aacucaccac uggcauagau cuggaucaac aauugguaaa gccuuugaag     1860

cuaccgucag gggcgccaag cguauggcag ugcucgggga cacagcuugg gauuucggaa     1920

guguaggcgg ugcccugaau agccuuggga aaggcaucca ccagaucuuc ggagcggccu     1980

uuaaguccuu guucggcggg augucuuggu uuucgcaaau ucugaucgga acucuccuga     2040

uguggcuugg ccugaacacc aaaaauggua gcauuuccuu gaugugucuc gcuuuagggg     2100

gagugcugau auuccuguca acggcaguua gugccgacgu cggcugcuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6106
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6106
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ggggcgccga caccagcgug gggaucgucg gacugcuccu uacaacugcu auggcagccg      120

agguuacgcg gcgcgguucc gcguacuaua uguaccugga ucgaaacgac gcuggcgaag      180

ccauuucuuu ccccaccaca uuggggauga auaagugcua uauacagauc auggauuuag      240

gacacaugug ugacgcaacu augucauacg agugcccuau gcuggaugaa ggcguggagc      300

cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau gguacuuguc      360

aucacaaaaa gggggaagcc cguagaagca ggcgggcugu gacacuaccg ucccauucua      420

cccgcaaacu ccaaacgaga ucgcagacuu ggcuggaguc aagggaguac accaagcacc      480

uuauucgggu ugaaaauugg aucuuucgca accccggauu cgcacuggcc gcugccgcga      540

uugcaugguu gcucggcagc aguacauccc agaaagugau cuaucugguc augauacugc      600

ucaucgcccc ugcuuacucu auucgaugca ucggcgugag caauagagau uuuguagagg      660

ggauguccgg agguacuugg guugacauug uguuagaaca uggcgggugu gucaccguga      720

uggcccagga uaagccaaca guggacaucg agcuggucac cacgacuguu ucaaacaugg      780

cagaggugag gaguuacugc uaugaagcua gcauaucuga uauggccucc gacucacggu      840

gucccacaca aggagaggcg uaccuugaca agcagagcga uacccaguau guaugcaaac      900

guacuuuggu ggaccgcggc ugggguaaug gguguggacu guucggcaag ggcagucuag      960

ucacaugcgc caaauuugca ugcucuaaga aaaugaccgg gaaguccauu cagccugaaa     1020

accucgagua cagaaucaug cugagcgugc acggaucgca acacucuggu augauuguua     1080

augauacggg ccaugaaacc gacgagaaca gggcuaaggu ggagaucaca ccaaauucac     1140

cccgggccga agcuacucuu gggggauucg gcucccuggg uuuggauugu gagccucgaa     1200

ccgggcucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac uggcuggucc     1260

auaaggaaug guuccacgac aucccacucc cguggcacgc cggcgcagau acuggaacac     1320

cccauuggaa caauaaagag gcccuugugg aauuuaagga cgcucacgcg aaacgccaga     1380

ccguaguugu gcugggcagc caggaggggg caguccauac cgccuuggcu ggagcccuag     1440

aggcagaaau ggauggugcc aagggcagac uguccucugg gcaccucaaa ugcaggcuga     1500

agauggacaa gcugcggcug aaaggaguga gcuauucauu guguacugcu gccuucacgu     1560

uuacaaagau uccugcagag acccuccaug gcacugucac cguggaaguu caguacgcgg     1620

guacagacgg gccaugcaaa gugcccgcuc aaauggccgu agauaugcag acccugacuc     1680

cugucggccg cuuaauaaca gcaaacccag ugaucacgga gaguaccgaa aacuccaaga     1740

ugaugcugga gcuagauccc ccguucggag acagcuacau uguuaucggc gugggggaga     1800

aaaagauaac ucaccacugg cauagaucug gaucaacaau ugguaaggcc uuugaagcua     1860

ccgucagggg cgccaaacgu auggcagugc ucggggacac agcuugggau uucggaagug     1920

uaggcggugc ccugaauagc cuugggaagg gcauccacca gaucuucgga gcggccuuua     1980

aauccuuguu cggcgggaug ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu     2040

ggcuuggccu gaacaccaag aaugguagca uuuccuugau gugucucgcu cuugggggag     2100

uguuaauauu ccugucaacg gcaguuagug ccgacgucgg cugcugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6107
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6107
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug gcagccgagg      120

uuacgaggcg ggguuccgcg uacuauaugu accuggaucg caacgacgcu ggcgaagcca      180

uuucuuuccc caccacauug gggaugaaua agugcuauau acagaucaug gauuuaggac      240

acauguguga cgcaacuaug ucauacgagu gcccuaugcu ggaugaaggc guggagccag      300

acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu acuugucauc      360

acaaaaaggg ggaagcccga cguagcagaa gggcugugac acuaccgucc cauucuaccc      420

ggaaacucca aacgcgcucg cagacuuggc uggagucaag agaguacacc aagcaccuua      480

uuaggguuga aaauuggauc uuucggaacc ccggauucgc acuggccgcu gccgcgauug      540

caugguugcu cggcagcagu acaucccaga aagugaucua ucuggucaug auacugcuca      600

ucgccccugc uuacucuauu cgcugcaucg gcgugagcaa ucgagauuuu guagagggga      660

uguccggagg uacuuggguu gacauugugu uagaacaugg cggguguguc accgugaugg      720

cccaggauaa gccaacagug gacaucgagc uggucaccac gacuguuuca aacauggcag      780

aggugagaag uuacugcuau gaagcuagca uaucugauau ggccuccgac ucaagguguc      840

ccacacaagg agaggcguac cuugacaagc agagcgauac ccaguaugua ugcaaacgga      900

cuuuggugga ccguggcugg gguaaugggu guggacuguu cggcaagggc agucuaguca      960

caugcgccaa auuugcaugc ucuaagaaaa ugaccgggaa guccauucag ccugaaaacc     1020

ucgaguaccg caucaugcug agcgugcacg gaucgcaaca cucugguaug auuguuaaug     1080

auacgggcca ugaaaccgac gagaacagag cuaaggugga gaucacacca aauuccccca     1140

gggccgaagc uacucuuggg ggauucggcu cacuggguuu ggauugugag ccucggaccg     1200

ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaaacacugg cugguccaua     1260

aggaaugguu ccacgacauc ccacucccgu ggcacgccgg cgcagauacu ggaacacccc     1320

auuggaacaa uaaagaggcc cuuguggaau uuaaggacgc ucacgcgaaa cgacagaccg     1380

uaguugugcu gggcagccag gagggggcag uccauaccgc cuuggcugga gcccuagagg     1440

cagaaaugga uggugccaag ggccgccugu ccucugggca ccucaaaugc agacugaaga     1500

uggacaagcu gaggcugaaa ggagugagcu auucauugug uacugcugcc uucacguuua     1560

caaagauucc ugcagagacc cuccauggca cugucaccgu ggaaguucag uacgcgggua     1620

cagacgggcc augcaaagug cccgcucaaa uggccguaga uaugcagacc cugacuccug     1680

ucggccgguu aauaacagca aacccaguga ucacggagag uaccgaaaac uccaagauga     1740

ugcuggagcu agaucccccg uucggagaca gcuacauugu uaucggcgug ggggagaaaa     1800

agauaacuca ccacuggcau cgcucuggau caacaauugg uaaggccuuu gaagcuaccg     1860

ucagaggcgc caaaaggaug gcagugcucg gggacacagc uugggauuuc ggaaguguag     1920

gcggugcccu gaauagccuu gggaagggca uccaccagau cuucggagcg gccuuuaaau     1980

ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc cugauguggc     2040

uuggccugaa caccaagaau gguagcauuu ccuugaugug ucucgcucuu gggggagugu     2100

uaauauuccu gucaacggca guuagugccg acgucggcug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6108
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6108
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gucggacugc uccuuacaac ugcuauggca gccgagguua      120

cgagaagggg uuccgcguac uauauguacc uggaucggaa cgacgcuggc gaagccauuu      180

cuuuccccac cacauugggg augaauaagu gcuauauaca gaucauggau uuaggacaca      240

ugugugacgc aacuauguca uacgagugcc cuaugcugga ugaaggcgug gagccagacg      300

auguagacug uuggugcaac accacaagua ccuggguggu cuaugguacu ugucaucaca      360

aaaaggggga agcccgccga agccguagag cugugacacu accgucccau ucuaccagga      420

aacuccaaac gcggucgcag acuuggcugg agucacgcga guacaccaag caccuuauua      480

gaguugaaaa uuggaucuuu aggaaccccg gauucgcacu ggccgcugcc gcgauugcau      540

gguugcucgg cagcaguaca ucccagaaag ugaucuaucu ggucaugaua cugcucaucg      600

ccccugcuua cucuauucgg ugcaucggcg ugagcaaucg cgauuuugua gaggggaugu      660

ccggagguac uuggguugac auuguguuag aacauggcgg gugugucacc gugauggccc      720

aggauaagcc aacaguggac aucgagcugg ucaccacgac uguuucaaac auggcagagg      780

ugcgaaguua cugcuaugaa gcuagcauau cugauauggc cuccgacuca agauguccca      840

cacaaggaga ggcguaccuu gacaagcaga gcgauaccca guauguaugc aaaaggacuu      900

ugguggaccg gggcuggggu aaugggugug gacuguucgg caagggcagu cuagucacau      960

gcgccaaauu ugcaugcucu aagaaaauga ccgggaaguc cauucagccu gaaaaccucg     1020

aguaccguau caugcugagc gugcacggau cgcaacacuc ugguaugauu guuaaugaua     1080

cgggccauga aaccgacgag aaccgcgcua agguggagau cacaccaaau ucccccagag     1140

ccgaagcuac ucuuggggga uucggcucac uggguuugga uugugagccu aggaccgggc     1200

ucgacuuuag ugaucuguau uacuuaacaa ugaacaauaa acacuggcug guccauaagg     1260

aaugguucca cgacauccca cucccguggc acgccggcgc agauacugga acaccccauu     1320

ggaacaauaa agaggcccuu guggaauuua aggacgcuca cgcgaaacgg cagaccguag     1380

uugugcuggg cagccaggag ggggcagucc auaccgccuu ggcuggagcc cuagaggcag     1440

aaauggaugg ugccaagggc cgacuguccu cugggcaccu caaaugccgc cugaagaugg     1500

acaagcugag acugaaagga gugagcuauu cauuguguac ugcugccuuc acguuuacaa     1560

agauuccugc agagacccuc cauggcacug ucaccgugga aguucaguac gcggguacag     1620

acgggccaug caaagugccc gcucaaaugg ccguagauau gcagacccug acuccugucg     1680

gcagguuaau aacagcaaac ccagugauca cggagaguac cgaaaacucc aagaugaugc     1740

uggagcuaga ucccccguuc ggagacagcu acauuguuau cggcgugggg gagaaaaaga     1800

uaacucacca cuggcaucgg ucuggaucaa caauugguaa ggccuuugaa gcuaccgucc     1860

gcggcgccaa aagaauggca gugcucgggg acacagcuug ggauuucgga aguguaggcg     1920

gugcccugaa uagccuuggg aagggcaucc accagaucuu cggagcggcc uuuaaauccu     1980

uguucggcgg gaugucuugg uuuucgcaaa uucugaucgg aacucuccug auguggcuug     2040

gccugaacac caagaauggu agcauuuccu ugaugugucu cgcucuuggg ggaguguuaa     2100

uauuccuguc aacggcaguu agugccgacg ucggcugcug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6109
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6109
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucguc gggcugcucc uuacaacugc uauggcagcc gagguuacga      120

gaaggggauc cgcguacuau auguaccugg aucggaacga cgcuggugaa gccauuucuu      180

uccccaccac auugggcaug aauaagugcu auauacagau cauggauuua gggcacaugu      240

gugacgcaac uaugucauac gagugcccua ugcuggauga aggaguggag ccagacgaug      300

uagacuguug gugcaacacc acaaguaccu ggguggucua uggcacuugu caucacaaaa      360

agggugaagc ccgccgaagc cguagagcug ugacacuacc gucccauucu accaggaaac      420

uccaaacgcg gucgcagacu uggcuggagu cacgcgagua caccaagcac cuuauuagag      480

uugaaaauug gaucuuuagg aaccccgggu ucgcacuggc cgcugccgcg auugcauggu      540

ugcucggaag caguacaucc cagaaaguga ucuaucuggu caugauacug cucaucgccc      600

cugcuuacuc uauucggugc aucggcguga gcaaucgcga uuuuguagag ggcauguccg      660

ggggaacuug gguugacauu guguuagaac augguggcug ugucaccgug auggcccagg      720

auaagccaac aguggacauc gagcugguca ccacgacugu uucaaacaug gcagaggugc      780

gaaguuacug cuaugaagcu agcauaucug auauggccuc cgacucaaga ugucccacac      840

aaggggaggc guaccuugac aagcagagcg auacccagua uguaugcaaa aggacuuugg      900

uggaccgggg auggggcaau gguugugggc uguucggaaa gggcagucua gucacaugcg      960

ccaaauuugc augcucuaag aaaaugaccg gcaaguccau ucagccugaa aaccucgagu     1020

accguaucau gcugagcgug cacgggucgc aacacucugg aaugauuguu aaugauacgg     1080

gucaugaaac cgacgagaac cgcgcuaagg uggagaucac accaaauucc cccagagccg     1140

aagcuacucu uggcggguuc ggaucacugg gcuuggauug ugagccuagg accggucucg     1200

acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc cauaaggaau     1260

gguuccacga caucccacuc ccguggcacg ccggggcaga uacuggcaca ccccauugga     1320

acaauaaaga ggcccuugug gaauuuaagg acgcucacgc gaaacggcag accguaguug     1380

ugcugggaag ccaggagggc gcaguccaua ccgccuuggc uggggcccua gaggcagaaa     1440

uggauggagc caagggucga cuguccucug gccaccucaa augccgccug aagauggaca     1500

aacuuagacu gaagggggug agcuauucau uguguacugc ugccuucacg uuuacaaaga     1560

uuccugcaga gacccuccau ggaacuguca ccguggaagu ucaguacgcg ggcacagacg     1620

guccaugcaa agugcccgcu caaauggccg uagauaugca gacccugacu ccugucggga     1680

gguuaauaac agcaaaccca gugaucacgg agaguaccga aaacuccaag augaugcugg     1740

agcuagaucc cccguucggc gacagcuaca uuguuaucgg agugggcgag aaaaagauaa     1800

cucaccacug gcaucggucu gggucaacaa uuggaaaggc cuuugaagcu accguccgcg     1860

gugccaaaag aauggcagug cucggcgaca cagcuuggga uuucgggagu guaggaggcg     1920

cccugaauag ccuugguaag gggauccacc agaucuucgg cgcggccuuu aaauccuugu     1980

ucggaggcau gucuugguuu ucgcaaauuc ugaucgggac ucuccugaug uggcuuggac     2040

ugaacaccaa gaauggcagc auuuccuuga ugugucucgc ucuggguggg guguuaauau     2100

uccugucaac ggcaguuagu gccgacgucg gaugcugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6110
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6110
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgucggg cugcuccuua caacugccau ggcugcagag guuacgagaa      120

ggggauccgc cuacuauaug uaccuggauc ggaacgacgc gggugaagcu auuucuuucc      180

ccaccacauu gggcaugaau aagugcuaua uacagaucau ggauuuaggg cacaugugug      240

acgccacuau gucauacgag ugcccuaugc uggaugaagg aguggagcca gacgauguag      300

acuguuggug caacaccaca aguaccuggg uggucuaugg cacuugucau cacaaaaagg      360

gugaagcacg ccgaagccgu agagccguga cacuaccguc ccauucuacc aggaaacucc      420

aaacgcgguc gcagacuugg cuggagucac gcgaguacac caagcaccuu auuagaguug      480

aaaauuggau cuuuaggaac cccggguucg cucuggcagc cgcugccauu gcgugguugc      540

ucggaagcag uacaucccag aaagugaucu aucuggucau gauacugcuc aucgcacccg      600

ccuacucuau ucggugcauc ggcgugagca aucgcgauuu uguagagggc auguccgggg      660

gaacuugggu ugacauugug uuagaacaug guggcugugu caccgugaug gcucaggaua      720

agccaacagu ggacaucgag cuggucacca cgacuguuuc aaacauggcc gaggugcgaa      780

guuacugcua ugaagcaagc auaucugaua uggcuuccga cucaagaugu cccacacaag      840

gggaggccua ccuugacaag cagagcgaua cccaguaugu augcaaaagg acuuuggugg      900

accggggaug gggcaauggu ugugggcugu ucggaaaggg cagucuaguc acaugcgcga      960

aauuugccug cucuaagaaa augaccggca aguccauuca gccugaaaac cucgaguacc     1020

guaucaugcu gagcgugcac gggucgcaac acucuggaau gauuguuaau gauacggguc     1080

augaaaccga cgagaaccgc gcaaaggugg agaucacacc aaauuccccc agagcugaag     1140

ccacucuugg cggguucgga ucacugggcu uggauuguga gccuaggacc ggucucgacu     1200

uuagugaucu guauuacuua acaaugaaca auaaacacug gcugguccau aaggaauggu     1260

uccacgacau cccacucccg uggcacgcug gggccgauac uggcaccccu cauuggaaca     1320

auaaagaggc acuuguggaa uuuaaggacg cccacgcuaa acggcagaca guaguugugc     1380

ugggaagcca ggagggcgcg guccauaccg cauuggccgg ggcucuagag gccgaaaugg     1440

auggagcaaa gggucgacug uccucuggcc accucaaaug ccgccugaag auggacaaac     1500

uuagacugaa gggggugagc uauucauugu guacugccgc uuucacguuu acaaagauuc     1560

cugccgagac ccuccaugga acugucaccg uggaaguuca guacgcaggc acagacgguc     1620

caugcaaagu gcccgcgcaa auggcuguag auaugcagac ccugacuccu gucgggaggu     1680

uaauaacagc caacccagug aucacggaga guaccgaaaa cuccaagaug augcuggagc     1740

uagauccccc guucggcgac agcuacauug uuaucggagu gggcgagaaa aagauaacuc     1800

accacuggca ucggucuggg ucaacaauug gaaaggcauu ugaagccacc guccgcggug     1860

cuaaaagaau ggccgugcuc ggcgacacag caugggauuu cgggagugua ggaggcgcuc     1920

ugaauagccu ugguaagggg auccaccaga ucuucggcgc cgcguuuaaa uccuuguucg     1980

gaggcauguc uugguuuucg caaauucuga ucgggacucu ccugaugugg cuuggacuga     2040

acaccaagaa uggcagcauu uccuugaugu gucucgcccu ggguggggug uuaauauucc     2100

ugucaacggc uguuagugca gacgucggau gcugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6111
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6111
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcagau gucggaugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6112
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6112
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagccguu acgagaaggg      120

gauccgcgua cuauauguac cuggaccgga acgaugcugg ugaggccauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

caacuauguc auacgaaugc ccuaugcugg acgagggagu ggaaccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aggcccgccg aagccguaga gcugugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gaaucacgcg aguacaccaa gcaccuuauu agaguugaga      480

auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcuu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agaaggcaug uccgggggaa      660

cuuggguuga uauuguguua gagcauggug gcugugucac cgugauggcc caggacaagc      720

caacagugga uaucgaacug gucaccacga cuguuucaaa cauggcagag gugcgaaguu      780

acugcuauga ggcuagcaua ucugacaugg ccuccgauuc aagauguccc acacaagggg      840

aagcguaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgccaaau      960

uugcaugcuc uaagaaaaug accggcaagu ccauucagcc ugagaaccuc gaauaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

agaccgauga aaaccgcgcu aagguggaga ucacaccaaa uucccccaga gccgaggcua     1140

cucuuggcgg guucggauca cugggcuugg acugugaacc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gagugguucc     1260

acgauauccc acucccgugg cacgccgggg cagacacugg cacaccccau uggaacaaua     1320

aagaagcccu uguggaguuu aaggaugcuc acgcgaaacg gcagaccgua guugugcugg     1380

gaagccagga aggcgcaguc cauaccgccu uggcuggggc ccuagaggca gagauggacg     1440

gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca aagauuccug     1560

cagaaacccu ccauggaacu gucaccgugg agguucagua cgcgggcaca gacgguccau     1620

gcaaagugcc cgcucaaaug gccguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagcaaa cccagugauc acggaaagua ccgagaacuc caagaugaug cuggaacuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggccuuuga ggcuaccguc cgcggugcca     1860

aaagaauggc agugcucggc gauacagcuu gggacuucgg gaguguagga ggcgcccuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua auauuccugu     2100

caacggcagu uagugccgau gucggaugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6113
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6113
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug gcagccgcgg      120

uuacgaggcg ggguuccgcu uacuauaugu accuggaucg caacgacgcc ggcgaggcaa      180

uuucuuuccc caccacauug gggaugaaua agugcuauau acagaucaug gauuuaggac      240

acauguguga cgccacuaug ucauacgaau gcccuaugcu ggaugagggc guggaaccag      300

acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu acuugucauc      360

acaaaaaggg ggaggcucga cguagcagaa gggcagugac acuaccgucc cauucuaccc      420

ggaaacucca aacgcgcucg cagacuuggc uggaaucaag agaguacacc aagcaccuua      480

uuaggguuga gaauuggauc uuucggaacc ccggauucgc ccuggcugcc gcggcaauug      540

ccugguugcu cggcagcagu acaucccaga aagugaucua ucuggucaug auacugcuca      600

ucgcuccugc cuacucuauu cgcugcaucg gcgugagcaa ucgagauuuu guagaaggga      660

uguccggagg uacuuggguu gacauugugu uagagcaugg cggguguguc accgugaugg      720

cacaggauaa gccaacagug gacaucgaac uggucaccac gacuguuuca aacauggcug      780

aggugagaag uuacugcuau gaggccagca uaucugauau ggcguccgac ucaagguguc      840

ccacacaagg agaagccuac cuugacaagc agagcgauac ccaguaugua ugcaaacgga      900

cuuuggugga ccguggcugg gguaaugggu guggacuguu cggcaagggc agucuaguca      960

caugcgcaaa auuugcuugc ucuaagaaaa ugaccgggaa guccauucag ccugagaacc     1020

ucgaauaccg caucaugcug agcgugcacg gaucgcaaca cucugguaug auuguuaaug     1080

auacgggcca ugagaccgac gaaaacagag ccaaggugga gaucacacca aauuccccca     1140

gggcugaggc cacucuuggg ggauucggcu cacuggguuu ggauugugaa ccucggaccg     1200

ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaaacacugg cugguccaua     1260

aggagugguu ccacgacauc ccacucccgu ggcacgcagg cgccgauacu ggaacacccc     1320

auuggaacaa uaaagaagcu cuuguggagu uuaaggacgc gcacgcaaaa cgacagaccg     1380

uaguugugcu gggcagccag gaaggggccg uccauaccgc uuuggccgga gcacuagagg     1440

ccgagaugga uggugcuaag ggccgccugu ccucugggca ccucaaaugc agacugaaga     1500

uggacaagcu gaggcugaaa ggagugagcu auucauugug uacugccgca uucacguuua     1560

caaagauucc ugcggaaacc cuccauggca cugucaccgu ggagguucag uacgcuggua     1620

cagacgggcc augcaaagug cccgcccaaa uggcaguaga uaugcagacc cugacuccug     1680

ucggccgguu aauaacagcc aacccaguga ucacggaaag uaccgagaac uccaagauga     1740

ugcuggaacu agaucccccg uucggagaca gcuacauugu uaucggcgug ggggagaaaa     1800

agauaacuca ccacuggcau cgcucuggau caacaauugg uaaggcuuuu gaggccaccg     1860

ucagaggcgc aaaaaggaug gcugugcucg gggacacagc cugggauuuc ggaaguguag     1920

gcggugcgcu gaauagccuu gggaagggca uccaccagau cuucggagcc gcuuuuaaau     1980

ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc cugauguggc     2040

uuggccugaa caccaagaau gguagcauuu ccuugaugug ucucgcacuu gggggagugu     2100

uaauauuccu gucaacggcc guuagugcug acgucggcug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6114
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6114
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc caggaagcgg cgcucucacg acgugcucac cguccaguuc cuuauccugg      120

ggauguuguu aaugacagga gguguuacuc gacguggcuc agccuacuau auguaccugg      180

auagaaacga cgcuggggag gcaauuaguu uucccacgac ccuaggaaug aauaaaugcu      240

auauacaaau cauggaucuc ggccauaugu gugacgccac aaugagcuac gaaugcccua      300

ugcuggauga ggguguggaa ccagacgaug uagacuguug gugcaacacu accuccacau      360

ggguggucua ugggaccugu caccauaaga aaggagaggc gaggcggucu cgcagagcug      420

ugacucuucc gucgcacuca acaaggaagc ugcagacccg gagccagacg ugguuggaaa      480

gucgcgagua cacuaaacau cucauucgag uugagaauug gaucuucaga aaccccggcu      540

uugcccuggc agccgcugca auugccuggc ugcucggcuc cucuaccagc cagaagguga      600

ucuauuuagu caugauacug cuuaucgcuc cugccuacuc cauuaggugc aucggggugu      660

caaaucggga uuucguagaa ggaaugagug guggcacaug gguugacauu guguuggagc      720

acgggggaug ugucacugug auggcgcaag auaagccaac cguggacauc gaacugguca      780

caaccacggu uagcaacaug gcagaggugc guucuuacug cuaugaggcc uccauaucag      840

auauggcuag cgacagucgc ugucccacuc agggcgaagc cuaccuagac aaacagucug      900

auacacagua uguaugcaag agaacccucg uggacagggg uugggggaau ggauguggcc      960

uguuuggcaa agggucccuu gucacuugcg caaaguucgc uugcagcaaa aagaugacag     1020

gaaagucgau ucaaccugag aaccuggaau accggaucau guugucugug cacgguuccc     1080

agcauucagg caugauuguu aaugauaccg ggcacgagac ggacgaaaac cgagccaaag     1140

uggagaucac cccaaauagu ccccgcgcgg aggccacacu cggaggcuuu gguagccugg     1200

gguuagauug ugaaccuaga acuggccugg acuucuccga ucucuauuac cuuaccauga     1260

acaauaagca uuggcugguc cacaaagagu gguuucacga caucccauug ccguggcaug     1320

caggagcuga uacaggcacu ccccacugga acaauaagga agcccuagug gaguucaaag     1380

acgcucaugc caagaggcag accguaguug ugcugggguc ucaggaagga gcaguccaca     1440

cagcccucgc uggugcgcug gaggcagaga uggauggcgc caaagggcgg cuuagcucag     1500

gacaucugaa gugccgcuug aagauggaca aacucagacu gaagggcgug aguuauuccu     1560

uauguaccgc ugccuuuacu uucacgaaaa uuccugcaga aacacugcac gguaccguca     1620

cuguggaggu ucaauacgcc gggaccgacg gcccaugcaa ggugcccgcu cagauggccg     1680

uagauaugca gacacuaacc ccugucggaa ggcucauaac ugcaaaccca gugaucacag     1740

aaagcacgga gaacucuaaa augaugcugg aacuugaucc cccguuuggc gacucauaca     1800

uuguuaucgg ggugggagag aagaagauaa cccaccauug gcaccguagu gguagcacua     1860

uuggcaaagc guucgaggcu acaguccgag gggccaagcg gauggcagug uugggagaca     1920

ccgccuggga uuucggcucc guaggugggg cucucaauuc ucugggcaaa ggaauccauc     1980

aaaucuuugg cgccgcauuc aagucgcugu uugggggaau gagcugguuc ucccagauuc     2040

uuaucggcac acuguugaug uggcucgguc ugaacacuaa aaauggguca auuaguuuaa     2100

ugugucuggc ucuaggaggc gugcucauau uucugucuac cgccguuagc gcggacgucg     2160

guugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6115
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6115
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc caggaagcgg cgcucucacg acgugcucac cguccaguuc cuuauccugg      120

ggauguuguu aaugacagga gccguuacuc gacgugguuc agcuuacuau auguaccugg      180

auagaaacga cgcaggcgag gccauuaguu uucccacgac ccuagggaug aauaaaugcu      240

auauacaaau cauggaucuc ggacauaugu gugacgcgac aaugagcuac gaaugcccua      300

ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacu accuccacau      360

ggguggucua ugguaccugu caccauaaga aaggggaggc uaggcggucu cgcagagccg      420

ugacucuucc gucgcacuca acaaggaagc ugcagacccg gagccagacg ugguuggaaa      480

gucgcgagua cacuaaacau cucauucgag uugagaauug gaucuucaga aaccccggau      540

uugcacuggc cgcugcagcc auugcuuggc ugcucggcuc cucuaccagc cagaagguga      600

ucuauuuagu caugauacug cuuaucgccc cugcguacuc cauuaggugc aucggcgugu      660

caaaucggga uuucguagaa gggaugagug gagguacaug gguugacauu guguuggagc      720

acggcgggug ugucacugug auggcacaag auaagccaac cguggacauc gaacugguca      780

caaccacggu uagcaacaug gccgaggugc guucuuacug cuaugaggcu uccauaucag      840

auauggccag cgacagucgc ugucccacuc agggagaagc auaccuagac aaacagucug      900

auacacagua uguaugcaag agaacccucg uggacagggg cugggguaau ggguguggac      960

uguuuggcaa aggcucccuu gucacuugcg cuaaguucgc cugcagcaaa aagaugacag     1020

ggaagucgau ucaaccugag aaccuggaau accggaucau guugucugug cacggauccc     1080

agcauucagg uaugauuguu aaugauaccg gccacgagac ggacgaaaac cgagcgaaag     1140

uggagaucac cccaaauagu ccccgcgccg aggcaacacu cgggggauuu ggcagccugg     1200

guuuagauug ugaaccuaga acugggcugg acuucuccga ucucuauuac cuuaccauga     1260

acaauaagca uuggcugguc cacaaagagu gguuucacga caucccauug ccguggcaug     1320

cuggcgccga uacaggaacu ccccacugga acaauaagga agcucuagug gaguucaaag     1380

acgcccaugc aaagaggcag accguaguug ugcugggcuc ucaggaaggg gccguccaca     1440

cagcucucgc gggagcacug gaggccgaga uggauggugc uaaaggccgg cuuagcucag     1500

ggcaucugaa gugccgcuug aagauggaca aacucagacu gaagggagug aguuauuccu     1560

uauguaccgc cgcauuuacu uucacgaaaa uuccugccga aacacugcac ggcaccguca     1620

cuguggaggu ucaauacgcu gguaccgacg ggccaugcaa ggugcccgcc cagauggcag     1680

uagauaugca gacacuaacc ccugucggca ggcucauaac ugcgaaccca gugaucacag     1740

aaagcacgga gaacucuaaa augaugcugg aacuugaucc cccguuugga gacucauaca     1800

uuguuaucgg cgugggggag aagaagauaa cccaccauug gcaccguagu ggaagcacua     1860

uugguaaggc uuucgaggcc acaguccggg gcgcaaaacg aauggccgug uugggggaca     1920

ccgcuuggga uuucggaucc guaggcggug cccucaauuc ucuggggaaa ggcauccauc     1980

aaaucuuugg agcagcuuuc aagucgcugu uuggcgggau gagcugguuc ucccagauuc     2040

uuaucggaac acuguugaug uggcucggcc ugaacacuaa aaaugguuca auuaguuuaa     2100

ugugucuggc ccuaggggga gugcucauau uucugucuac cgcgguuagc gccgacgucg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6116
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6116
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacauag      720

uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca guggacauug      780

agcuggucac cacgacuguu ucaaauaugg cugaagugcg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu ggaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuugugg     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguaguugu gcugggcagc caggaggggg     1440

ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggaa acccuccaug     1620

gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag acccugacuc cugucggcag guuaauuaca gccaacccag     1740

ugaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggacac agccugggau uucggaagug uaggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag ugcugauuuu ccugucaacg gccguuagug     2160

cugacugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6117
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6117
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uuaacgccag aaaggagaaa aagaggcggg gcgcugacac cagcgugggg auagucggac      120

ugcuccuuac aacugcaaug gccgcggaag uuacgcgccg agguuccgcu uacuauaugu      180

accuggaucg uaaugacgcc ggcgaggcaa ucucuuuccc caccacauug gggaugaaca      240

aaugcuauau ucagaucaug gauuuaggac acauguguga cgccacuaug ucauacgaau      300

gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc aauaccacaa      360

guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggcuaga aggagccggc      420

gcgcagugac acuaccgucc cauucuacca gaaagcucca aacgaggucg cagacuuggc      480

uggagucacg ggaauacacc aagcaccuua uucgcguuga gaacuggauc uuucgaaauc      540

ccggauucgc ccuggcugcc gcggcaauag ccugguugcu cggcagcagu acaucccaga      600

aagugaucua ucuggucaug auucugcuca ucgcuccugc cuacucuauu agaugcaucg      660

gcgugagcaa cagggauuuu guagaaggga uguccggagg uacuuggguu gacauagugu      720

uagagcaugg cggguguguc accgugaugg cacaggauaa gccaacagug gacauugagc      780

uggucaccac gacuguuuca aauauggcug aagugcggag uuacugcuau gaggccagca      840

ucucugauau ggcguccgac ucacguuguc ccacacaagg agaagccuac cuugacaaac      900

agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg gguaacgggu      960

guggacuguu cggcaaaggc agucuaguca caugcgcaaa guuugcuugc ucuaagaaaa     1020

ugaccgggaa guccauucag ccugagaauc ucgaauacag gaucaugcug agcgugcacg     1080

gaucgcaaca cucugguaug aucguuaacg auacgggcca ugagaccgac gagaaucggg     1140

ccaaagugga aauuacacca aacucacccc gagcugaggc cacucuuggg ggauucggcu     1200

cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug uauuacuuaa     1260

caaugaauaa caagcacugg cugguccaua aagagugguu ccacgacaua ccacucccgu     1320

ggcacgcagg cgccgauacu ggaacacccc auuggaauaa caaggaagcu cuuguggagu     1380

uuaaagacgc gcacgcaaag agacagaccg uaguugugcu gggcagccag gagggggccg     1440

uccauaccgc uuuggccgga gcacuagaag ccgagaugga uggugcuaag ggcaggcugu     1500

ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag ggagugagcu     1560

auucauugug uacugccgca uucacguuua caaaaauccc ugcggaaacc cuccauggca     1620

cugucaccgu ggagguucag uacgcuggua cagacgggcc augcaaggug cccgcccaaa     1680

uggcaguaga uaugcagacc cugacuccug ucggcagauu aauuacagcc aacccaguga     1740

ucacggaaag uaccgaaaau uccaagauga ugcuggagcu agaucccccg uucggagaca     1800

gcuacauagu uauuggcgug ggggagaaaa agaucacuca ccacuggcau aggucuggau     1860

caacaaucgg uaaggcuuuu gaggccaccg uccguggcgc aaaacggaug gcugugcucg     1920

gggacacagc cugggauuuc ggaaguguag gcggugcgcu gaacagccuu gggaaaggca     1980

uucaccagau cuucggagcc gcuuuuaagu ccuuguucgg cgggaugucu ugguuuucgc     2040

aaauucugau aggaacucuc cugauguggc uuggccugaa uaccaagaac gguagcaucu     2100

ccuugaugug ucucgcauua gggggagugc ugauuuuccu gucaacggcc guuagugcug     2160

acugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6118
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6118
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaaaaag aggcggggcg cugacaccag cguggggauu gucggacugc      120

uccuuacaac ugcaauggcc gcggaaguua cgcgccgagg uuccgcuuac uauauguacc      180

uggaucguaa ugacgccggc gaggcaauau cuuuccccac cacauugggg augaacaaau      240

gcuauaucca gauuauggau uuaggacaca ugugugacgc cacuauguca uacgaaugcc      300

cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau accacaagua      360

ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuagaagg agccggcgcg      420

cagugacacu accgucccau ucuaccagaa agcuccaaac gaggucgcag acuuggcugg      480

agucacggga auacaccaag caccuuaucc gcguugagaa cuggauuuuu cgaaaucccg      540

gauucgcccu ggcugccgcg gcaaucgccu gguugcucgg cagcaguaca ucccagaaag      600

ugauauaucu ggucaugauc cugcucauug cuccugccua cucuaucaga ugcauuggcg      660

ugagcaacag ggauuuugua gaagggaugu ccggagguac uuggguugac aucguguuag      720

agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac auagagcugg      780

ucaccacgac uguuucaaau auggcugaag ugcggaguua cugcuaugag gccagcauuu      840

cugauauggc guccgacuca cguuguccca cacaaggaga agccuaccuu gacaaacaga      900

gcgauaccca guauguaugc aagcgcacuu ugguggacag aggcuggggu aacgggugug      960

gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu aagaaaauga     1020

ccgggaaguc cauccagccu gagaaucucg aauacaggau uaugcugagc gugcacggau     1080

cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag aaucgggcca     1140

aaguggaaau cacaccaaac ucaccccgag cugaggccac ucuuggggga uucggcuccc     1200

uggguuugga uugugaaccu cgcaccgggc ucgacuuuag ugaucuguau uacuuaacaa     1260

ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauucca cucccguggc     1320

acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu guggaguuua     1380

aagacgcgca cgcaaagaga cagaccguag uugugcuggg cagccaggag ggggccgucc     1440

auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc aggcuguccu     1500

cugggcaccu caaaugccgg cugaagaugg acaaacuucg ccugaaggga gugagcuauu     1560

cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc cauggcacug     1620

ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc gcccaaaugg     1680

caguagauau gcagacccug acuccugucg gcagauuaau cacagccaac ccagugauua     1740

cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc ggagacagcu     1800

acaucguuau aggcgugggg gagaaaaaga uuacucacca cuggcauagg ucuggaucaa     1860

caaucgguaa ggcuuuugag gccaccgucc guggcgcaaa acggauggcu gugcucgggg     1920

acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg aaaggcaucc     1980

accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg uuuucgcaaa     2040

uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu agcauauccu     2100

ugaugugucu cgcauuaggg ggagugcuga ucuuccuguc aacggccguu agugcugacu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6119
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6119
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaaaaagagg cggggcgcug acaccagcgu ggggaucguc ggacugcucc      120

uuacaacugc aauggccgcg gaaguuacgc gccgagguuc cgcuuacuau auguaccugg      180

aucguaauga cgccggcgag gcaauuucuu uccccaccac auuggggaug aacaaaugcu      240

auauacagau cauggauuua ggacacaugu gugacgccac uaugucauac gaaugcccua      300

ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaauacc acaaguaccu      360

ggguggucua ugguacuugu caucacaaga aaggggaggc uagaaggagc cggcgcgcag      420

ugacacuacc gucccauucu accagaaagc uccaaacgag gucgcagacu uggcuggagu      480

cacgggaaua caccaagcac cuuauucgcg uugagaacug gaucuuucga aaucccggau      540

ucgcccuggc ugccgcggca auugccuggu ugcucggcag caguacaucc cagaaaguga      600

ucuaucuggu caugauacug cucaucgcuc cugccuacuc uauuagaugc aucggcguga      660

gcaacaggga uuuuguagaa gggauguccg gagguacuug gguugacauu guguuagagc      720

auggcgggug ugucaccgug auggcacagg auaagccaac aguggacauc gagcugguca      780

ccacgacugu uucaaauaug gcugaagugc ggaguuacug cuaugaggcc agcauaucug      840

auauggcguc cgacucacgu ugucccacac aaggagaagc cuaccuugac aaacagagcg      900

auacccagua uguaugcaag cgcacuuugg uggacagagg cugggguaac ggguguggac      960

uguucggcaa aggcagucua gucacaugcg caaaguuugc uugcucuaag aaaaugaccg     1020

ggaaguccau ucagccugag aaucucgaau acaggaucau gcugagcgug cacggaucgc     1080

aacacucugg uaugauuguu aacgauacgg gccaugagac cgacgagaau cgggccaaag     1140

uggaaaucac accaaacuca ccccgagcug aggccacucu ugggggauuc ggcucccugg     1200

guuuggauug ugaaccucgc accgggcucg acuuuaguga ucuguauuac uuaacaauga     1260

auaacaagca cuggcugguc cauaaagagu gguuccacga caucccacuc ccguggcacg     1320

caggcgccga uacuggaaca ccccauugga auaacaagga agcucuugug gaguuuaaag     1380

acgcgcacgc aaagagacag accguaguug ugcugggcag ccaggagggg gccguccaua     1440

ccgcuuuggc cggagcacua gaagccgaga uggauggugc uaagggcagg cuguccucug     1500

ggcaccucaa augccggcug aagauggaca aacuucgccu gaagggagug agcuauucau     1560

uguguacugc cgcauucacg uuuacaaaaa uuccugcgga aacccuccau ggcacuguca     1620

ccguggaggu ucaguacgcu gguacagacg ggccaugcaa ggugcccgcc caaauggcag     1680

uagauaugca gacccugacu ccugucggca gauuaauaac agccaaccca gugaucacgg     1740

aaaguaccga aaauuccaag augaugcugg agcuagaucc cccguucgga gacagcuaca     1800

uuguuaucgg cgugggggag aaaaagauaa cucaccacug gcauaggucu ggaucaacaa     1860

uugguaaggc uuuugaggcc accguccgug gcgcaaaacg gauggcugug cucggggaca     1920

cagccuggga uuucggaagu guaggcggug cgcugaacag ccuugggaaa ggcauccacc     1980

agaucuucgg agccgcuuuu aaguccuugu ucggcgggau gucuugguuu ucgcaaauuc     2040

ugaucggaac ucuccugaug uggcuuggcc ugaauaccaa gaacgguagc auuuccuuga     2100

ugugucucgc auuaggggga gugcugauau uccugucaac ggccguuagu gcugacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6120
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6120
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa aaagaggcgg ggcgcugaca ccagcguggg gaucgucgga cugcuccuua      120

caacugcaau ggccgcggaa guuacgcgcc gagguuccgc uuacuauaug uaccuggauc      180

guaacgacgc cggcgaggca auuucuuucc ccaccacauu ggggaugaau aaaugcuaua      240

uacagaucau ggauuuagga cacaugugug acgccacuau gucauacgaa ugcccuaugc      300

uggaugaggg cguggaacca gacgauguag acuguuggug caacaccaca aguaccuggg      360

uggucuaugg uacuugucau cacaagaaag gggaggcuag aaggagccgg cgcgcaguga      420

cacuaccguc ccauucuacc agaaagcucc aaacgagguc gcagacuugg cuggagucac      480

gggaauacac caagcaccuu auucgcguug agaauuggau cuuucgaaac cccggauucg      540

cccuggcugc cgcggcaauu gccugguugc ucggcagcag uacaucccag aaagugaucu      600

aucuggucau gauacugcuc aucgcuccug ccuacucuau uagaugcauc ggcgugagca      660

auagggauuu uguagaaggg auguccggag guacuugggu ugacauugug uuagagcaug      720

gcgggugugu caccgugaug gcacaggaua agccaacagu ggacaucgag cuggucacca      780

cgacuguuuc aaacauggcu gaagugcgga guuacugcua ugaggccagc auaucugaua      840

uggcguccga cucacguugu cccacacaag gagaagccua ccuugacaaa cagagcgaua      900

cccaguaugu augcaagcgc acuuuggugg acagaggcug ggguaauggg uguggacugu      960

ucggcaaagg cagucuaguc acaugcgcaa aguuugcuug cucuaagaaa augaccggga     1020

aguccauuca gccugagaac cucgaauaca ggaucaugcu gagcgugcac ggaucgcaac     1080

acucugguau gauuguuaau gauacgggcc augagaccga cgagaaccgg gccaaagugg     1140

aaaucacacc aaauucaccc cgagcugagg ccacucuugg gggauucggc ucccuggguu     1200

uggauuguga accucgcacc gggcucgacu uuagugaucu guauuacuua acaaugaaca     1260

auaagcacug gcugguccau aaagaguggu uccacgacau cccacucccg uggcacgcag     1320

gcgccgauac uggaacaccc cauuggaaca auaaggaagc ucuuguggag uuuaaagacg     1380

cgcacgcaaa gagacagacc guaguugugc ugggcagcca ggagggggcc guccauaccg     1440

cuuuggccgg agcacuagaa gccgagaugg auggugcuaa gggcaggcug uccucugggc     1500

accucaaaug ccggcugaag auggacaaac uucgccugaa gggagugagc uauucauugu     1560

guacugccgc auucacguuu acaaaaauuc cugcggaaac ccuccauggc acugucaccg     1620

uggagguuca guacgcuggu acagacgggc caugcaaggu gcccgcccaa auggcaguag     1680

auaugcagac ccugacuccu gucggcagau uaauaacagc caacccagug aucacggaaa     1740

guaccgagaa cuccaagaug augcuggagc uagauccccc guucggagac agcuacauug     1800

uuaucggcgu gggggagaaa aagauaacuc accacuggca uaggucugga ucaacaauug     1860

guaaggcuuu ugaagccacc guccguggcg caaaacggau ggcugugcuc ggggacacag     1920

ccugggauuu cggaagugua ggcggugcgc ugaauagccu ugggaaaggc auccaccaga     1980

ucuucggagc cgcuuuuaag uccuuguucg gcgggauguc uugguuuucg caaauucuga     2040

ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu uccuugaugu     2100

gucucgcauu agggggagug cugauauucc ugucaacggc cguuagugcu gacugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6121
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6121
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaaaaa gaggcggggc gccgacacca gcguggggau cgucggacug cuccuuacaa      120

cugcuauggc agccgaaguu acgcgccgag guuccgcgua cuauauguac cuggaucgua      180

acgacgcugg cgaggccauu ucuuucccca ccacauuggg gaugaauaaa ugcuauauac      240

agaucaugga uuuaggacac augugugacg caacuauguc auacgaaugc ccuaugcugg      300

augagggcgu ggaaccagac gauguagacu guuggugcaa caccacaagu accugggugg      360

ucuaugguac uugucaucac aagaaagggg aggccagaag gagccggcgc gcugugacac      420

uaccguccca uucuaccaga aagcuccaaa cgaggucgca gacuuggcug gagucacggg      480

aauacaccaa gcaccuuauu cgcguugaga auuggaucuu ucgaaacccc ggauucgcac      540

uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaaa gugaucuauc      600

uggucaugau acugcucauc gccccugcuu acucuauuag augcaucggc gugagcaaua      660

gggauuuugu agaagggaug uccggaggua cuuggguuga cauuguguua gagcauggcg      720

ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug gucaccacga      780

cuguuucaaa cauggcagaa gugcggaguu acugcuauga ggcuagcaua ucugauaugg      840

ccuccgacuc acguuguccc acacaaggag aagcguaccu ugacaaacag agcgauaccc      900

aguauguaug caagcgcacu uugguggaca gaggcugggg uaaugggugu ggacuguucg      960

gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaagaaaaug accgggaagu     1020

ccauucagcc ugagaaccuc gaauacagga ucaugcugag cgugcacgga ucgcaacacu     1080

cugguaugau uguuaaugau acgggccaug agaccgacga gaaccgggcu aaaguggaaa     1140

ucacaccaaa uucaccccga gccgaggcua cucuuggggg auucggcucc cuggguuugg     1200

auugugaacc ucgcaccggg cucgacuuua gugaucugua uuacuuaaca augaacaaua     1260

agcacuggcu gguccauaaa gagugguucc acgacauccc acucccgugg cacgccggcg     1320

cagauacugg aacaccccau uggaacaaua aggaagcccu uguggaguuu aaagacgcuc     1380

acgcgaagag acagaccgua guugugcugg gcagccagga gggggcaguc cauaccgccu     1440

uggcuggagc ccuagaagca gagauggaug gugccaaggg caggcugucc ucugggcacc     1500

ucaaaugccg gcugaagaug gacaaacuuc gccugaaggg agugagcuau ucauugugua     1560

cugcugccuu cacguuuaca aaaauuccug cagaaacccu ccauggcacu gucaccgugg     1620

agguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug gccguagaua     1680

ugcagacccu gacuccuguc ggcagauuaa uaacagcaaa cccagugauc acggaaagua     1740

ccgagaacuc caagaugaug cuggagcuag aucccccguu cggagacagc uacauuguua     1800

ucggcguggg ggagaaaaag auaacucacc acuggcauag gucuggauca acaauuggua     1860

aagccuuuga agcuaccguc cguggcgcca agcggauggc agugcucggg gacacagcuu     1920

gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc caccagaucu     1980

ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa auucugaucg     2040

gaacucuccu gauguggcuu ggccugaaca ccaagaaugg uagcauuucc uugauguguc     2100

ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6122
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6122
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaaaaagag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc cuuacaacug      120

cuauggcagc cgaaguuacg cggcgcgguu ccgcguacua uauguaccug gaucgaaacg      180

acgcuggcga ggccauuucu uuccccacca cauuggggau gaauaaaugc uauauacaga      240

ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgaaugcccu augcuggaug      300

agggcgugga accagacgau guagacuguu ggugcaacac cacaaguacc uggguggucu      360

augguacuug ucaucacaag aaaggggagg cccguagaag caggcgggcu gugacacuac      420

cgucccauuc uacccgcaag cuccaaacga gaucgcagac uuggcuggag ucaagggaau      480

acaccaagca ccuuauucgg guugagaauu ggaucuuucg caaccccgga uucgcacugg      540

ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug aucuaucugg      600

ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug agcaauagag      660

auuuuguaga agggaugucc ggagguacuu ggguugacau uguguuagag cauggcgggu      720

gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc accacgacug      780

uuucaaacau ggcagaagug aggaguuacu gcuaugaggc uagcauaucu gauauggccu      840

ccgacucacg gugucccaca caaggagaag cguaccuuga caaacagagc gauacccagu      900

auguaugcaa gcguacuuug guggaccgcg gcugggguaa ugggugugga cuguucggca      960

aaggcagucu agucacaugc gccaaguuug caugcucuaa gaaaaugacc gggaagucca     1020

uucagccuga gaaccucgaa uacagaauca ugcugagcgu gcacggaucg caacacucug     1080

guaugauugu uaaugauacg ggccaugaga ccgacgagaa cagggcuaaa guggaaauca     1140

caccaaauuc accccgggcc gaggcuacuc uugggggauu cggcucccug gguuuggauu     1200

gugaaccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug aacaauaagc     1260

acuggcuggu ccauaaagag ugguuccacg acaucccacu cccguggcac gccggcgcag     1320

auacuggaac accccauugg aacaauaagg aagcccuugu ggaguuuaaa gacgcucacg     1380

cgaagcgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau accgccuugg     1440

cuggagcccu agaagcagag auggauggug ccaagggcag acuguccucu gggcaccuca     1500

aaugcaggcu gaagauggac aaacuucggc ugaagggagu gagcuauuca uuguguacug     1560

cugccuucac guuuacaaaa auuccugcag aaacccucca uggcacuguc accguggagg     1620

uucaguacgc ggguacagac gggccaugca aggugcccgc ucaaauggcc guagauaugc     1680

agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg gaaaguaccg     1740

agaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac auuguuaucg     1800

gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca auugguaaag     1860

ccuuugaagc uaccgucagg ggcgccaagc guauggcagu gcucggggac acagcuuggg     1920

auuucggaag uguaggcggu gcccugaaua gccuugggaa aggcauccac cagaucuucg     1980

gagcggccuu uaaguccuug uucggcggga ugucuugguu uucgcaaauu cugaucggaa     2040

cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug augugucucg     2100

cuuuaggggg agugcugaua uuccugucaa cggcaguuag ugccgacuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6123
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6123
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagaag gggcgccgac accagcgugg ggaucgucgg acugcuccuu acaacugcua      120

uggcagccga aguuacgcgg cgcgguuccg cguacuauau guaccuggau cgaaacgacg      180

cuggcgaggc cauuucuuuc cccaccacau uggggaugaa uaagugcuau auacagauca      240

uggauuuagg acacaugugu gacgcaacua ugucauacga augcccuaug cuggaugagg      300

gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg guggucuaug      360

guacuuguca ucacaaaaag ggggaggccc guagaagcag gcgggcugug acacuaccgu      420

cccauucuac ccgcaaacuc caaacgagau cgcagacuug gcuggaguca agggaauaca      480

ccaagcaccu uauucggguu gagaauugga ucuuucgcaa ccccggauuc gcacuggccg      540

cugccgcgau ugcaugguug cucggcagca guacauccca gaaggugauc uaucugguca      600

ugauacugcu caucgccccu gcuuacucua uucgaugcau cggcgugagc aauagagauu      660

uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau ggcgggugug      720

ucaccgugau ggcccaggau aaaccaacag uggacaucga gcuggucacc acgacuguuu      780

caaacauggc agaagugagg aguuacugcu augaggcuag cauaucugau auggccuccg      840

acucacggug ucccacacaa ggagaagcgu accuugacaa gcagagcgau acccaguaug      900

uaugcaaacg uacuuuggug gaccgcggcu gggguaaugg guguggacug uucggcaagg      960

gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa gaugaccggg aaauccauuc     1020

agccugagaa ccucgaauac agaaucaugc ugagcgugca cggaucgcaa cacucuggua     1080

ugauuguuaa ugauacgggc caugagaccg acgagaacag ggcuaaggug gaaaucacac     1140

caaauucacc ccgggccgag gcuacucuug ggggauucgg cucccugggu uuggauugug     1200

aaccucgaac cgggcucgac uuuagugauc uguauuacuu aacaaugaac aauaaacacu     1260

ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgcc ggcgcagaua     1320

cuggaacacc ccauuggaac aauaaagaag cccuugugga guuuaaggac gcucacgcga     1380

aacgccagac cguaguugug cugggcagcc aggagggggc aguccauacc gccuuggcug     1440

gagcccuaga agcagagaug gauggugcca agggcagacu guccucuggg caccucaagu     1500

gcaggcugaa aauggacaag cugcggcuga aaggagugag cuauucauug uguacugcug     1560

ccuucacguu uacaaagauu ccugcagaaa cccuccaugg cacugucacc guggagguuc     1620

aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua gauaugcaga     1680

cccugacucc ugucggccgc uuaauaacag caaacccagu gaucacggaa aguaccgaga     1740

acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu guuaucggcg     1800

ugggggaaaa gaaaauaacu caccacuggc auagaucugg aucaacaauu gguaaggccu     1860

uugaggcuac cgucaggggc gccaaacgua uggcagugcu cggggacaca gcuugggauu     1920

ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag aucuucggag     1980

cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug aucggaacuc     2040

uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug ugucucgcuc     2100

uugggggagu guuaauauuc cugucaacgg caguuagugc cgacugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6124
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6124
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagaagggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca acugcuaugg      120

cagccgaggu uacgcggcgc gguuccgcgu acuauaugua ccuggaucga aacgacgcug      180

gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua cagaucaugg      240

auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug gaugaaggcg      300

uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug gucuauggua      360

cuugucauca caaaaagggg gaagcccgua gaagcaggcg ggcugugaca cuaccguccc      420

auucuacccg caaacuccaa acgagaucgc agacuuggcu ggagucaagg gaguacacca      480

agcaccuuau ucggguugaa aauuggaucu uucgcaaccc cggauucgca cuggccgcug      540

ccgcgauugc augguugcuc ggcagcagua caucccagaa ggugaucuau cuggucauga      600

uacugcucau cgccccugcu uacucuauuc gaugcaucgg cgugagcaau agagauuuug      660

uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc ggguguguca      720

ccgugauggc ccaggauaaa ccaacagugg acaucgagcu ggucaccacg acuguuucaa      780

acauggcaga ggugaggagu uacugcuaug aagcuagcau aucugauaug gccuccgacu      840

cacggugucc cacacaagga gaggcguacc uugacaagca gagcgauacc caguauguau      900

gcaaacguac uuugguggac cgcggcuggg guaaugggug uggacuguuc ggcaagggca      960

gucuagucac augcgccaaa uuugcaugcu cuaagaagau gaccgggaaa uccauucagc     1020

cugaaaaccu cgaguacaga aucaugcuga gcgugcacgg aucgcaacac ucugguauga     1080

uuguuaauga uacgggccau gaaaccgacg agaacagggc uaagguggag aucacaccaa     1140

auucaccccg ggccgaagcu acucuugggg gauucggcuc ccuggguuug gauugugagc     1200

cucgaaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau aaacacuggc     1260

ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc gcagauacug     1320

gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu cacgcgaaac     1380

gccagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc uuggcuggag     1440

cccuagaggc agaaauggau ggugccaagg gcagacuguc cucugggcac cucaagugca     1500

ggcugaaaau ggacaagcug cggcugaaag gagugagcua uucauugugu acugcugccu     1560

ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug gaaguucagu     1620

acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau augcagaccc     1680

ugacuccugu cggccgcuua auaacagcaa acccagugau cacggagagu accgaaaacu     1740

ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu aucggcgugg     1800

gggagaagaa aauaacucac cacuggcaua gaucuggauc aacaauuggu aaggccuuug     1860

aagcuaccgu caggggcgcc aaacguaugg cagugcucgg ggacacagcu ugggauuucg     1920

gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc uucggagcgg     1980

ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc ggaacucucc     2040

ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu cucgcucuug     2100

ggggaguguu aauauuccug ucaacggcag uuagugccga cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6125
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6125
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gaaggggcgc cgacaccagc guggggaucg ucggacugcu ccuuacaacu gcuauggcag      120

ccgagguuac gcggcgcggu uccgcguacu auauguaccu ggaucgaaac gacgcuggcg      180

aagccauuuc uuuccccacc acauugggga ugaauaaaug cuauauacag aucauggauu      240

uaggacacau gugugacgca acuaugucau acgagugccc uaugcuggau gaaggcgugg      300

agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc uaugguacuu      360

gucaucacaa gaaaggggaa gcccguagaa gcaggcgggc ugugacacua ccgucccauu      420

cuacccgcaa gcuccaaacg agaucgcaga cuuggcugga gucaagggag uacaccaaac      480

accuuauucg gguugaaaau uggaucuuuc gcaaccccgg auucgcacug gccgcugccg      540

cgauugcaug guugcucggc agcaguacau cccagaaggu gaucuaucug gucaugauac      600

ugcucaucgc cccugcuuac ucuauucgau gcaucggcgu gagcaauaga gauuuuguag      660

aggggauguc cggagguacu uggguugaca uuguguuaga acauggcggg ugugucaccg      720

ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu guuucaaaca      780

uggcagaggu gaggaguuac ugcuaugaag cuagcauauc ugauauggcc uccgacucac      840

ggugucccac acaaggagag gcguaccuug acaaacagag cgauacccag uauguaugca      900

agcguacuuu gguggaccgc ggcuggggua augggugugg acuguucggc aaaggcaguc      960

uagucacaug cgccaaguuu gcaugcucua aaaagaugac cgggaagucc auucagccug     1020

aaaaccucga guacagaauc augcugagcg ugcacggauc gcaacacucu gguaugauug     1080

uuaaugauac gggccaugaa accgacgaga acagggcuaa aguggagauc acaccaaauu     1140

caccccgggc cgaagcuacu cuugggggau ucggcucccu ggguuuggau ugugagccuc     1200

gaaccgggcu cgacuuuagu gaucuguauu acuuaacaau gaacaauaag cacuggcugg     1260

uccauaaaga augguuccac gacaucccac ucccguggca cgccggcgca gauacuggaa     1320

caccccauug gaacaauaag gaggcccuug uggaauuuaa agacgcucac gcgaagcgcc     1380

agaccguagu ugugcugggc agccaggagg gggcagucca uaccgccuug gcuggagccc     1440

uagaggcaga aauggauggu gccaaaggca gacuguccuc ugggcaccuc aagugcaggc     1500

ugaagaugga caaacuucgg cugaagggag ugagcuauuc auuguguacu gcugccuuca     1560

cguuuacaaa aauuccugca gagacccucc auggcacugu caccguggaa guucaguacg     1620

cggguacaga cgggccaugc aaggugcccg cucaaauggc cguagauaug cagacccuga     1680

cuccugucgg ccgcuuaaua acagcaaacc cagugaucac ggagaguacc gaaaacucca     1740

aaaugaugcu ggagcuagau cccccguucg gagacagcua cauuguuauc ggcguggggg     1800

agaagaagau aacucaccac uggcauagau cuggaucaac aauugguaaa gccuuugaag     1860

cuaccgucag gggcgccaag cguauggcag ugcucgggga cacagcuugg gauuucggaa     1920

guguaggcgg ugcccugaau agccuuggga aaggcaucca ccagaucuuc ggagcggccu     1980

uuaaguccuu guucggcggg augucuuggu uuucgcaaau ucugaucgga acucuccuga     2040

uguggcuugg ccugaacacc aaaaauggua gcauuuccuu gaugugucuc gcuuuagggg     2100

gagugcugau auuccuguca acggcaguua gugccgacug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6126
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6126
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ggggcgccga caccagcgug gggaucgucg gacugcuccu uacaacugcu auggcagccg      120

agguuacgcg gcgcgguucc gcguacuaua uguaccugga ucgaaacgac gcuggcgaag      180

ccauuucuuu ccccaccaca uuggggauga auaagugcua uauacagauc auggauuuag      240

gacacaugug ugacgcaacu augucauacg agugcccuau gcuggaugaa ggcguggagc      300

cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau gguacuuguc      360

aucacaaaaa gggggaagcc cguagaagca ggcgggcugu gacacuaccg ucccauucua      420

cccgcaaacu ccaaacgaga ucgcagacuu ggcuggaguc aagggaguac accaagcacc      480

uuauucgggu ugaaaauugg aucuuucgca accccggauu cgcacuggcc gcugccgcga      540

uugcaugguu gcucggcagc aguacauccc agaaagugau cuaucugguc augauacugc      600

ucaucgcccc ugcuuacucu auucgaugca ucggcgugag caauagagau uuuguagagg      660

ggauguccgg agguacuugg guugacauug uguuagaaca uggcgggugu gucaccguga      720

uggcccagga uaagccaaca guggacaucg agcuggucac cacgacuguu ucaaacaugg      780

cagaggugag gaguuacugc uaugaagcua gcauaucuga uauggccucc gacucacggu      840

gucccacaca aggagaggcg uaccuugaca agcagagcga uacccaguau guaugcaaac      900

guacuuuggu ggaccgcggc ugggguaaug gguguggacu guucggcaag ggcagucuag      960

ucacaugcgc caaauuugca ugcucuaaga aaaugaccgg gaaguccauu cagccugaaa     1020

accucgagua cagaaucaug cugagcgugc acggaucgca acacucuggu augauuguua     1080

augauacggg ccaugaaacc gacgagaaca gggcuaaggu ggagaucaca ccaaauucac     1140

cccgggccga agcuacucuu gggggauucg gcucccuggg uuuggauugu gagccucgaa     1200

ccgggcucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac uggcuggucc     1260

auaaggaaug guuccacgac aucccacucc cguggcacgc cggcgcagau acuggaacac     1320

cccauuggaa caauaaagag gcccuugugg aauuuaagga cgcucacgcg aaacgccaga     1380

ccguaguugu gcugggcagc caggaggggg caguccauac cgccuuggcu ggagcccuag     1440

aggcagaaau ggauggugcc aagggcagac uguccucugg gcaccucaaa ugcaggcuga     1500

agauggacaa gcugcggcug aaaggaguga gcuauucauu guguacugcu gccuucacgu     1560

uuacaaagau uccugcagag acccuccaug gcacugucac cguggaaguu caguacgcgg     1620

guacagacgg gccaugcaaa gugcccgcuc aaauggccgu agauaugcag acccugacuc     1680

cugucggccg cuuaauaaca gcaaacccag ugaucacgga gaguaccgaa aacuccaaga     1740

ugaugcugga gcuagauccc ccguucggag acagcuacau uguuaucggc gugggggaga     1800

aaaagauaac ucaccacugg cauagaucug gaucaacaau ugguaaggcc uuugaagcua     1860

ccgucagggg cgccaaacgu auggcagugc ucggggacac agcuugggau uucggaagug     1920

uaggcggugc ccugaauagc cuugggaagg gcauccacca gaucuucgga gcggccuuua     1980

aauccuuguu cggcgggaug ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu     2040

ggcuuggccu gaacaccaag aaugguagca uuuccuugau gugucucgcu cuugggggag     2100

uguuaauauu ccugucaacg gcaguuagug ccgacugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6127
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6127
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug gcagccgagg      120

uuacgaggcg ggguuccgcg uacuauaugu accuggaucg caacgacgcu ggcgaagcca      180

uuucuuuccc caccacauug gggaugaaua agugcuauau acagaucaug gauuuaggac      240

acauguguga cgcaacuaug ucauacgagu gcccuaugcu ggaugaaggc guggagccag      300

acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu acuugucauc      360

acaaaaaggg ggaagcccga cguagcagaa gggcugugac acuaccgucc cauucuaccc      420

ggaaacucca aacgcgcucg cagacuuggc uggagucaag agaguacacc aagcaccuua      480

uuaggguuga aaauuggauc uuucggaacc ccggauucgc acuggccgcu gccgcgauug      540

caugguugcu cggcagcagu acaucccaga aagugaucua ucuggucaug auacugcuca      600

ucgccccugc uuacucuauu cgcugcaucg gcgugagcaa ucgagauuuu guagagggga      660

uguccggagg uacuuggguu gacauugugu uagaacaugg cggguguguc accgugaugg      720

cccaggauaa gccaacagug gacaucgagc uggucaccac gacuguuuca aacauggcag      780

aggugagaag uuacugcuau gaagcuagca uaucugauau ggccuccgac ucaagguguc      840

ccacacaagg agaggcguac cuugacaagc agagcgauac ccaguaugua ugcaaacgga      900

cuuuggugga ccguggcugg gguaaugggu guggacuguu cggcaagggc agucuaguca      960

caugcgccaa auuugcaugc ucuaagaaaa ugaccgggaa guccauucag ccugaaaacc     1020

ucgaguaccg caucaugcug agcgugcacg gaucgcaaca cucugguaug auuguuaaug     1080

auacgggcca ugaaaccgac gagaacagag cuaaggugga gaucacacca aauuccccca     1140

gggccgaagc uacucuuggg ggauucggcu cacuggguuu ggauugugag ccucggaccg     1200

ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaaacacugg cugguccaua     1260

aggaaugguu ccacgacauc ccacucccgu ggcacgccgg cgcagauacu ggaacacccc     1320

auuggaacaa uaaagaggcc cuuguggaau uuaaggacgc ucacgcgaaa cgacagaccg     1380

uaguugugcu gggcagccag gagggggcag uccauaccgc cuuggcugga gcccuagagg     1440

cagaaaugga uggugccaag ggccgccugu ccucugggca ccucaaaugc agacugaaga     1500

uggacaagcu gaggcugaaa ggagugagcu auucauugug uacugcugcc uucacguuua     1560

caaagauucc ugcagagacc cuccauggca cugucaccgu ggaaguucag uacgcgggua     1620

cagacgggcc augcaaagug cccgcucaaa uggccguaga uaugcagacc cugacuccug     1680

ucggccgguu aauaacagca aacccaguga ucacggagag uaccgaaaac uccaagauga     1740

ugcuggagcu agaucccccg uucggagaca gcuacauugu uaucggcgug ggggagaaaa     1800

agauaacuca ccacuggcau cgcucuggau caacaauugg uaaggccuuu gaagcuaccg     1860

ucagaggcgc caaaaggaug gcagugcucg gggacacagc uugggauuuc ggaaguguag     1920

gcggugcccu gaauagccuu gggaagggca uccaccagau cuucggagcg gccuuuaaau     1980

ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc cugauguggc     2040

uuggccugaa caccaagaau gguagcauuu ccuugaugug ucucgcucuu gggggagugu     2100

uaauauuccu gucaacggca guuagugccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6128
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6128
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gucggacugc uccuuacaac ugcuauggca gccgagguua      120

cgagaagggg uuccgcguac uauauguacc uggaucggaa cgacgcuggc gaagccauuu      180

cuuuccccac cacauugggg augaauaagu gcuauauaca gaucauggau uuaggacaca      240

ugugugacgc aacuauguca uacgagugcc cuaugcugga ugaaggcgug gagccagacg      300

auguagacug uuggugcaac accacaagua ccuggguggu cuaugguacu ugucaucaca      360

aaaaggggga agcccgccga agccguagag cugugacacu accgucccau ucuaccagga      420

aacuccaaac gcggucgcag acuuggcugg agucacgcga guacaccaag caccuuauua      480

gaguugaaaa uuggaucuuu aggaaccccg gauucgcacu ggccgcugcc gcgauugcau      540

gguugcucgg cagcaguaca ucccagaaag ugaucuaucu ggucaugaua cugcucaucg      600

ccccugcuua cucuauucgg ugcaucggcg ugagcaaucg cgauuuugua gaggggaugu      660

ccggagguac uuggguugac auuguguuag aacauggcgg gugugucacc gugauggccc      720

aggauaagcc aacaguggac aucgagcugg ucaccacgac uguuucaaac auggcagagg      780

ugcgaaguua cugcuaugaa gcuagcauau cugauauggc cuccgacuca agauguccca      840

cacaaggaga ggcguaccuu gacaagcaga gcgauaccca guauguaugc aaaaggacuu      900

ugguggaccg gggcuggggu aaugggugug gacuguucgg caagggcagu cuagucacau      960

gcgccaaauu ugcaugcucu aagaaaauga ccgggaaguc cauucagccu gaaaaccucg     1020

aguaccguau caugcugagc gugcacggau cgcaacacuc ugguaugauu guuaaugaua     1080

cgggccauga aaccgacgag aaccgcgcua agguggagau cacaccaaau ucccccagag     1140

ccgaagcuac ucuuggggga uucggcucac uggguuugga uugugagccu aggaccgggc     1200

ucgacuuuag ugaucuguau uacuuaacaa ugaacaauaa acacuggcug guccauaagg     1260

aaugguucca cgacauccca cucccguggc acgccggcgc agauacugga acaccccauu     1320

ggaacaauaa agaggcccuu guggaauuua aggacgcuca cgcgaaacgg cagaccguag     1380

uugugcuggg cagccaggag ggggcagucc auaccgccuu ggcuggagcc cuagaggcag     1440

aaauggaugg ugccaagggc cgacuguccu cugggcaccu caaaugccgc cugaagaugg     1500

acaagcugag acugaaagga gugagcuauu cauuguguac ugcugccuuc acguuuacaa     1560

agauuccugc agagacccuc cauggcacug ucaccgugga aguucaguac gcggguacag     1620

acgggccaug caaagugccc gcucaaaugg ccguagauau gcagacccug acuccugucg     1680

gcagguuaau aacagcaaac ccagugauca cggagaguac cgaaaacucc aagaugaugc     1740

uggagcuaga ucccccguuc ggagacagcu acauuguuau cggcgugggg gagaaaaaga     1800

uaacucacca cuggcaucgg ucuggaucaa caauugguaa ggccuuugaa gcuaccgucc     1860

gcggcgccaa aagaauggca gugcucgggg acacagcuug ggauuucgga aguguaggcg     1920

gugcccugaa uagccuuggg aagggcaucc accagaucuu cggagcggcc uuuaaauccu     1980

uguucggcgg gaugucuugg uuuucgcaaa uucugaucgg aacucuccug auguggcuug     2040

gccugaacac caagaauggu agcauuuccu ugaugugucu cgcucuuggg ggaguguuaa     2100

uauuccuguc aacggcaguu agugccgacu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6129
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6129
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucguc gggcugcucc uuacaacugc uauggcagcc gagguuacga      120

gaaggggauc cgcguacuau auguaccugg aucggaacga cgcuggugaa gccauuucuu      180

uccccaccac auugggcaug aauaagugcu auauacagau cauggauuua gggcacaugu      240

gugacgcaac uaugucauac gagugcccua ugcuggauga aggaguggag ccagacgaug      300

uagacuguug gugcaacacc acaaguaccu ggguggucua uggcacuugu caucacaaaa      360

agggugaagc ccgccgaagc cguagagcug ugacacuacc gucccauucu accaggaaac      420

uccaaacgcg gucgcagacu uggcuggagu cacgcgagua caccaagcac cuuauuagag      480

uugaaaauug gaucuuuagg aaccccgggu ucgcacuggc cgcugccgcg auugcauggu      540

ugcucggaag caguacaucc cagaaaguga ucuaucuggu caugauacug cucaucgccc      600

cugcuuacuc uauucggugc aucggcguga gcaaucgcga uuuuguagag ggcauguccg      660

ggggaacuug gguugacauu guguuagaac augguggcug ugucaccgug auggcccagg      720

auaagccaac aguggacauc gagcugguca ccacgacugu uucaaacaug gcagaggugc      780

gaaguuacug cuaugaagcu agcauaucug auauggccuc cgacucaaga ugucccacac      840

aaggggaggc guaccuugac aagcagagcg auacccagua uguaugcaaa aggacuuugg      900

uggaccgggg auggggcaau gguugugggc uguucggaaa gggcagucua gucacaugcg      960

ccaaauuugc augcucuaag aaaaugaccg gcaaguccau ucagccugaa aaccucgagu     1020

accguaucau gcugagcgug cacgggucgc aacacucugg aaugauuguu aaugauacgg     1080

gucaugaaac cgacgagaac cgcgcuaagg uggagaucac accaaauucc cccagagccg     1140

aagcuacucu uggcggguuc ggaucacugg gcuuggauug ugagccuagg accggucucg     1200

acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc cauaaggaau     1260

gguuccacga caucccacuc ccguggcacg ccggggcaga uacuggcaca ccccauugga     1320

acaauaaaga ggcccuugug gaauuuaagg acgcucacgc gaaacggcag accguaguug     1380

ugcugggaag ccaggagggc gcaguccaua ccgccuuggc uggggcccua gaggcagaaa     1440

uggauggagc caagggucga cuguccucug gccaccucaa augccgccug aagauggaca     1500

aacuuagacu gaagggggug agcuauucau uguguacugc ugccuucacg uuuacaaaga     1560

uuccugcaga gacccuccau ggaacuguca ccguggaagu ucaguacgcg ggcacagacg     1620

guccaugcaa agugcccgcu caaauggccg uagauaugca gacccugacu ccugucggga     1680

gguuaauaac agcaaaccca gugaucacgg agaguaccga aaacuccaag augaugcugg     1740

agcuagaucc cccguucggc gacagcuaca uuguuaucgg agugggcgag aaaaagauaa     1800

cucaccacug gcaucggucu gggucaacaa uuggaaaggc cuuugaagcu accguccgcg     1860

gugccaaaag aauggcagug cucggcgaca cagcuuggga uuucgggagu guaggaggcg     1920

cccugaauag ccuugguaag gggauccacc agaucuucgg cgcggccuuu aaauccuugu     1980

ucggaggcau gucuugguuu ucgcaaauuc ugaucgggac ucuccugaug uggcuuggac     2040

ugaacaccaa gaauggcagc auuuccuuga ugugucucgc ucuggguggg guguuaauau     2100

uccugucaac ggcaguuagu gccgacugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6130
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6130
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgucggg cugcuccuua caacugccau ggcugcagag guuacgagaa      120

ggggauccgc cuacuauaug uaccuggauc ggaacgacgc gggugaagcu auuucuuucc      180

ccaccacauu gggcaugaau aagugcuaua uacagaucau ggauuuaggg cacaugugug      240

acgccacuau gucauacgag ugcccuaugc uggaugaagg aguggagcca gacgauguag      300

acuguuggug caacaccaca aguaccuggg uggucuaugg cacuugucau cacaaaaagg      360

gugaagcacg ccgaagccgu agagccguga cacuaccguc ccauucuacc aggaaacucc      420

aaacgcgguc gcagacuugg cuggagucac gcgaguacac caagcaccuu auuagaguug      480

aaaauuggau cuuuaggaac cccggguucg cucuggcagc cgcugccauu gcgugguugc      540

ucggaagcag uacaucccag aaagugaucu aucuggucau gauacugcuc aucgcacccg      600

ccuacucuau ucggugcauc ggcgugagca aucgcgauuu uguagagggc auguccgggg      660

gaacuugggu ugacauugug uuagaacaug guggcugugu caccgugaug gcucaggaua      720

agccaacagu ggacaucgag cuggucacca cgacuguuuc aaacauggcc gaggugcgaa      780

guuacugcua ugaagcaagc auaucugaua uggcuuccga cucaagaugu cccacacaag      840

gggaggccua ccuugacaag cagagcgaua cccaguaugu augcaaaagg acuuuggugg      900

accggggaug gggcaauggu ugugggcugu ucggaaaggg cagucuaguc acaugcgcga      960

aauuugccug cucuaagaaa augaccggca aguccauuca gccugaaaac cucgaguacc     1020

guaucaugcu gagcgugcac gggucgcaac acucuggaau gauuguuaau gauacggguc     1080

augaaaccga cgagaaccgc gcaaaggugg agaucacacc aaauuccccc agagcugaag     1140

ccacucuugg cggguucgga ucacugggcu uggauuguga gccuaggacc ggucucgacu     1200

uuagugaucu guauuacuua acaaugaaca auaaacacug gcugguccau aaggaauggu     1260

uccacgacau cccacucccg uggcacgcug gggccgauac uggcaccccu cauuggaaca     1320

auaaagaggc acuuguggaa uuuaaggacg cccacgcuaa acggcagaca guaguugugc     1380

ugggaagcca ggagggcgcg guccauaccg cauuggccgg ggcucuagag gccgaaaugg     1440

auggagcaaa gggucgacug uccucuggcc accucaaaug ccgccugaag auggacaaac     1500

uuagacugaa gggggugagc uauucauugu guacugccgc uuucacguuu acaaagauuc     1560

cugccgagac ccuccaugga acugucaccg uggaaguuca guacgcaggc acagacgguc     1620

caugcaaagu gcccgcgcaa auggcuguag auaugcagac ccugacuccu gucgggaggu     1680

uaauaacagc caacccagug aucacggaga guaccgaaaa cuccaagaug augcuggagc     1740

uagauccccc guucggcgac agcuacauug uuaucggagu gggcgagaaa aagauaacuc     1800

accacuggca ucggucuggg ucaacaauug gaaaggcauu ugaagccacc guccgcggug     1860

cuaaaagaau ggccgugcuc ggcgacacag caugggauuu cgggagugua ggaggcgcuc     1920

ugaauagccu ugguaagggg auccaccaga ucuucggcgc cgcguuuaaa uccuuguucg     1980

gaggcauguc uugguuuucg caaauucuga ucgggacucu ccugaugugg cuuggacuga     2040

acaccaagaa uggcagcauu uccuugaugu gucucgcccu ggguggggug uuaauauucc     2100

ugucaacggc uguuagugca gacugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  6131
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6131
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcagau ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6132
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6132
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagccguu acgagaaggg      120

gauccgcgua cuauauguac cuggaccgga acgaugcugg ugaggccauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

caacuauguc auacgaaugc ccuaugcugg acgagggagu ggaaccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aggcccgccg aagccguaga gcugugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gaaucacgcg aguacaccaa gcaccuuauu agaguugaga      480

auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcuu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agaaggcaug uccgggggaa      660

cuuggguuga uauuguguua gagcauggug gcugugucac cgugauggcc caggacaagc      720

caacagugga uaucgaacug gucaccacga cuguuucaaa cauggcagag gugcgaaguu      780

acugcuauga ggcuagcaua ucugacaugg ccuccgauuc aagauguccc acacaagggg      840

aagcguaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgccaaau      960

uugcaugcuc uaagaaaaug accggcaagu ccauucagcc ugagaaccuc gaauaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

agaccgauga aaaccgcgcu aagguggaga ucacaccaaa uucccccaga gccgaggcua     1140

cucuuggcgg guucggauca cugggcuugg acugugaacc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gagugguucc     1260

acgauauccc acucccgugg cacgccgggg cagacacugg cacaccccau uggaacaaua     1320

aagaagcccu uguggaguuu aaggaugcuc acgcgaaacg gcagaccgua guugugcugg     1380

gaagccagga aggcgcaguc cauaccgccu uggcuggggc ccuagaggca gagauggacg     1440

gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca aagauuccug     1560

cagaaacccu ccauggaacu gucaccgugg agguucagua cgcgggcaca gacgguccau     1620

gcaaagugcc cgcucaaaug gccguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagcaaa cccagugauc acggaaagua ccgagaacuc caagaugaug cuggaacuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggccuuuga ggcuaccguc cgcggugcca     1860

aaagaauggc agugcucggc gauacagcuu gggacuucgg gaguguagga ggcgcccuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua auauuccugu     2100

caacggcagu uagugccgau ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6133
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6133
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug gcagccgcgg      120

uuacgaggcg ggguuccgcu uacuauaugu accuggaucg caacgacgcc ggcgaggcaa      180

uuucuuuccc caccacauug gggaugaaua agugcuauau acagaucaug gauuuaggac      240

acauguguga cgccacuaug ucauacgaau gcccuaugcu ggaugagggc guggaaccag      300

acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu acuugucauc      360

acaaaaaggg ggaggcucga cguagcagaa gggcagugac acuaccgucc cauucuaccc      420

ggaaacucca aacgcgcucg cagacuuggc uggaaucaag agaguacacc aagcaccuua      480

uuaggguuga gaauuggauc uuucggaacc ccggauucgc ccuggcugcc gcggcaauug      540

ccugguugcu cggcagcagu acaucccaga aagugaucua ucuggucaug auacugcuca      600

ucgcuccugc cuacucuauu cgcugcaucg gcgugagcaa ucgagauuuu guagaaggga      660

uguccggagg uacuuggguu gacauugugu uagagcaugg cggguguguc accgugaugg      720

cacaggauaa gccaacagug gacaucgaac uggucaccac gacuguuuca aacauggcug      780

aggugagaag uuacugcuau gaggccagca uaucugauau ggcguccgac ucaagguguc      840

ccacacaagg agaagccuac cuugacaagc agagcgauac ccaguaugua ugcaaacgga      900

cuuuggugga ccguggcugg gguaaugggu guggacuguu cggcaagggc agucuaguca      960

caugcgcaaa auuugcuugc ucuaagaaaa ugaccgggaa guccauucag ccugagaacc     1020

ucgaauaccg caucaugcug agcgugcacg gaucgcaaca cucugguaug auuguuaaug     1080

auacgggcca ugagaccgac gaaaacagag ccaaggugga gaucacacca aauuccccca     1140

gggcugaggc cacucuuggg ggauucggcu cacuggguuu ggauugugaa ccucggaccg     1200

ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaaacacugg cugguccaua     1260

aggagugguu ccacgacauc ccacucccgu ggcacgcagg cgccgauacu ggaacacccc     1320

auuggaacaa uaaagaagcu cuuguggagu uuaaggacgc gcacgcaaaa cgacagaccg     1380

uaguugugcu gggcagccag gaaggggccg uccauaccgc uuuggccgga gcacuagagg     1440

ccgagaugga uggugcuaag ggccgccugu ccucugggca ccucaaaugc agacugaaga     1500

uggacaagcu gaggcugaaa ggagugagcu auucauugug uacugccgca uucacguuua     1560

caaagauucc ugcggaaacc cuccauggca cugucaccgu ggagguucag uacgcuggua     1620

cagacgggcc augcaaagug cccgcccaaa uggcaguaga uaugcagacc cugacuccug     1680

ucggccgguu aauaacagcc aacccaguga ucacggaaag uaccgagaac uccaagauga     1740

ugcuggaacu agaucccccg uucggagaca gcuacauugu uaucggcgug ggggagaaaa     1800

agauaacuca ccacuggcau cgcucuggau caacaauugg uaaggcuuuu gaggccaccg     1860

ucagaggcgc aaaaaggaug gcugugcucg gggacacagc cugggauuuc ggaaguguag     1920

gcggugcgcu gaauagccuu gggaagggca uccaccagau cuucggagcc gcuuuuaaau     1980

ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc cugauguggc     2040

uuggccugaa caccaagaau gguagcauuu ccuugaugug ucucgcacuu gggggagugu     2100

uaauauuccu gucaacggcc guuagugcug acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6134
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6134
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc caggaagcgg cgcucucacg acgugcucac cguccaguuc cuuauccugg      120

ggauguuguu aaugacagga gguguuacuc gacguggcuc agccuacuau auguaccugg      180

auagaaacga cgcuggggag gcaauuaguu uucccacgac ccuaggaaug aauaaaugcu      240

auauacaaau cauggaucuc ggccauaugu gugacgccac aaugagcuac gaaugcccua      300

ugcuggauga ggguguggaa ccagacgaug uagacuguug gugcaacacu accuccacau      360

ggguggucua ugggaccugu caccauaaga aaggagaggc gaggcggucu cgcagagcug      420

ugacucuucc gucgcacuca acaaggaagc ugcagacccg gagccagacg ugguuggaaa      480

gucgcgagua cacuaaacau cucauucgag uugagaauug gaucuucaga aaccccggcu      540

uugcccuggc agccgcugca auugccuggc ugcucggcuc cucuaccagc cagaagguga      600

ucuauuuagu caugauacug cuuaucgcuc cugccuacuc cauuaggugc aucggggugu      660

caaaucggga uuucguagaa ggaaugagug guggcacaug gguugacauu guguuggagc      720

acgggggaug ugucacugug auggcgcaag auaagccaac cguggacauc gaacugguca      780

caaccacggu uagcaacaug gcagaggugc guucuuacug cuaugaggcc uccauaucag      840

auauggcuag cgacagucgc ugucccacuc agggcgaagc cuaccuagac aaacagucug      900

auacacagua uguaugcaag agaacccucg uggacagggg uugggggaau ggauguggcc      960

uguuuggcaa agggucccuu gucacuugcg caaaguucgc uugcagcaaa aagaugacag     1020

gaaagucgau ucaaccugag aaccuggaau accggaucau guugucugug cacgguuccc     1080

agcauucagg caugauuguu aaugauaccg ggcacgagac ggacgaaaac cgagccaaag     1140

uggagaucac cccaaauagu ccccgcgcgg aggccacacu cggaggcuuu gguagccugg     1200

gguuagauug ugaaccuaga acuggccugg acuucuccga ucucuauuac cuuaccauga     1260

acaauaagca uuggcugguc cacaaagagu gguuucacga caucccauug ccguggcaug     1320

caggagcuga uacaggcacu ccccacugga acaauaagga agcccuagug gaguucaaag     1380

acgcucaugc caagaggcag accguaguug ugcugggguc ucaggaagga gcaguccaca     1440

cagcccucgc uggugcgcug gaggcagaga uggauggcgc caaagggcgg cuuagcucag     1500

gacaucugaa gugccgcuug aagauggaca aacucagacu gaagggcgug aguuauuccu     1560

uauguaccgc ugccuuuacu uucacgaaaa uuccugcaga aacacugcac gguaccguca     1620

cuguggaggu ucaauacgcc gggaccgacg gcccaugcaa ggugcccgcu cagauggccg     1680

uagauaugca gacacuaacc ccugucggaa ggcucauaac ugcaaaccca gugaucacag     1740

aaagcacgga gaacucuaaa augaugcugg aacuugaucc cccguuuggc gacucauaca     1800

uuguuaucgg ggugggagag aagaagauaa cccaccauug gcaccguagu gguagcacua     1860

uuggcaaagc guucgaggcu acaguccgag gggccaagcg gauggcagug uugggagaca     1920

ccgccuggga uuucggcucc guaggugggg cucucaauuc ucugggcaaa ggaauccauc     1980

aaaucuuugg cgccgcauuc aagucgcugu uugggggaau gagcugguuc ucccagauuc     2040

uuaucggcac acuguugaug uggcucgguc ugaacacuaa aaauggguca auuaguuuaa     2100

ugugucuggc ucuaggaggc gugcucauau uucugucuac cgccguuagc gcggacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6135
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6135
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc caggaagcgg cgcucucacg acgugcucac cguccaguuc cuuauccugg      120

ggauguuguu aaugacagga gccguuacuc gacgugguuc agcuuacuau auguaccugg      180

auagaaacga cgcaggcgag gccauuaguu uucccacgac ccuagggaug aauaaaugcu      240

auauacaaau cauggaucuc ggacauaugu gugacgcgac aaugagcuac gaaugcccua      300

ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacu accuccacau      360

ggguggucua ugguaccugu caccauaaga aaggggaggc uaggcggucu cgcagagccg      420

ugacucuucc gucgcacuca acaaggaagc ugcagacccg gagccagacg ugguuggaaa      480

gucgcgagua cacuaaacau cucauucgag uugagaauug gaucuucaga aaccccggau      540

uugcacuggc cgcugcagcc auugcuuggc ugcucggcuc cucuaccagc cagaagguga      600

ucuauuuagu caugauacug cuuaucgccc cugcguacuc cauuaggugc aucggcgugu      660

caaaucggga uuucguagaa gggaugagug gagguacaug gguugacauu guguuggagc      720

acggcgggug ugucacugug auggcacaag auaagccaac cguggacauc gaacugguca      780

caaccacggu uagcaacaug gccgaggugc guucuuacug cuaugaggcu uccauaucag      840

auauggccag cgacagucgc ugucccacuc agggagaagc auaccuagac aaacagucug      900

auacacagua uguaugcaag agaacccucg uggacagggg cugggguaau ggguguggac      960

uguuuggcaa aggcucccuu gucacuugcg cuaaguucgc cugcagcaaa aagaugacag     1020

ggaagucgau ucaaccugag aaccuggaau accggaucau guugucugug cacggauccc     1080

agcauucagg uaugauuguu aaugauaccg gccacgagac ggacgaaaac cgagcgaaag     1140

uggagaucac cccaaauagu ccccgcgccg aggcaacacu cgggggauuu ggcagccugg     1200

guuuagauug ugaaccuaga acugggcugg acuucuccga ucucuauuac cuuaccauga     1260

acaauaagca uuggcugguc cacaaagagu gguuucacga caucccauug ccguggcaug     1320

cuggcgccga uacaggaacu ccccacugga acaauaagga agcucuagug gaguucaaag     1380

acgcccaugc aaagaggcag accguaguug ugcugggcuc ucaggaaggg gccguccaca     1440

cagcucucgc gggagcacug gaggccgaga uggauggugc uaaaggccgg cuuagcucag     1500

ggcaucugaa gugccgcuug aagauggaca aacucagacu gaagggagug aguuauuccu     1560

uauguaccgc cgcauuuacu uucacgaaaa uuccugccga aacacugcac ggcaccguca     1620

cuguggaggu ucaauacgcu gguaccgacg ggccaugcaa ggugcccgcc cagauggcag     1680

uagauaugca gacacuaacc ccugucggca ggcucauaac ugcgaaccca gugaucacag     1740

aaagcacgga gaacucuaaa augaugcugg aacuugaucc cccguuugga gacucauaca     1800

uuguuaucgg cgugggggag aagaagauaa cccaccauug gcaccguagu ggaagcacua     1860

uugguaaggc uuucgaggcc acaguccggg gcgcaaaacg aauggccgug uugggggaca     1920

ccgcuuggga uuucggaucc guaggcggug cccucaauuc ucuggggaaa ggcauccauc     1980

aaaucuuugg agcagcuuuc aagucgcugu uuggcgggau gagcugguuc ucccagauuc     2040

uuaucggaac acuguugaug uggcucggcc ugaacacuaa aaaugguuca auuaguuuaa     2100

ugugucuggc ccuaggggga gugcucauau uucugucuac cgcgguuagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6136
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6136
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacauag      720

uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca guggacauug      780

agcuggucac cacgacuguu ucaaauaugg cugaagugcg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu ggaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuugugg     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguaguugu gcugggcagc caggaggggg     1440

ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggaa acccuccaug     1620

gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag acccugacuc cugucggcag guuaauuaca gccaacccag     1740

ugaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggacac agccugggau uucggaagug uaggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag ugcugauuuu ccugucaacg gccguuagug     2160

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6137
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6137
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaaaaag aggcggggcg cugacaccag cguggggauu gucggacugc      120

uccuuacaac ugcaauggcc gcggaaguua cgcgccgagg uuccgcuuac uauauguacc      180

uggaucguaa ugacgccggc gaggcaauau cuuuccccac cacauugggg augaacaaau      240

gcuauaucca gauuauggau uuaggacaca ugugugacgc cacuauguca uacgaaugcc      300

cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaau accacaagua      360

ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcuagaagg agccggcgcg      420

cagugacacu accgucccau ucuaccagaa agcuccaaac gaggucgcag acuuggcugg      480

agucacggga auacaccaag caccuuaucc gcguugagaa cuggauuuuu cgaaaucccg      540

gauucgcccu ggcugccgcg gcaaucgccu gguugcucgg cagcaguaca ucccagaaag      600

ugauauaucu ggucaugauc cugcucauug cuccugccua cucuaucaga ugcauuggcg      660

ugagcaacag ggauuuugua gaagggaugu ccggagguac uuggguugac aucguguuag      720

agcauggcgg gugugucacc gugauggcac aggauaagcc aacaguggac auagagcugg      780

ucaccacgac uguuucaaau auggcugaag ugcggaguua cugcuaugag gccagcauuu      840

cugauauggc guccgacuca cguuguccca cacaaggaga agccuaccuu gacaaacaga      900

gcgauaccca guauguaugc aagcgcacuu ugguggacag aggcuggggu aacgggugug      960

gacuguucgg caaaggcagu cuagucacau gcgcaaaguu ugcuugcucu aagaaaauga     1020

ccgggaaguc cauccagccu gagaaucucg aauacaggau uaugcugagc gugcacggau     1080

cgcaacacuc ugguaugauc guuaacgaua cgggccauga gaccgacgag aaucgggcca     1140

aaguggaaau cacaccaaac ucaccccgag cugaggccac ucuuggggga uucggcuccc     1200

uggguuugga uugugaaccu cgcaccgggc ucgacuuuag ugaucuguau uacuuaacaa     1260

ugaauaacaa gcacuggcug guccauaaag agugguucca cgacauucca cucccguggc     1320

acgcaggcgc cgauacugga acaccccauu ggaauaacaa ggaagcucuu guggaguuua     1380

aagacgcgca cgcaaagaga cagaccguag uugugcuggg cagccaggag ggggccgucc     1440

auaccgcuuu ggccggagca cuagaagccg agauggaugg ugcuaagggc aggcuguccu     1500

cugggcaccu caaaugccgg cugaagaugg acaaacuucg ccugaaggga gugagcuauu     1560

cauuguguac ugccgcauuc acguuuacaa aaauaccugc ggaaacccuc cauggcacug     1620

ucaccgugga gguucaguac gcugguacag acgggccaug caaggugccc gcccaaaugg     1680

caguagauau gcagacccug acuccugucg gcagauuaau cacagccaac ccagugauua     1740

cggaaaguac cgaaaauucc aagaugaugc uggagcuaga ucccccguuc ggagacagcu     1800

acaucguuau aggcgugggg gagaaaaaga uuacucacca cuggcauagg ucuggaucaa     1860

caaucgguaa ggcuuuugag gccaccgucc guggcgcaaa acggauggcu gugcucgggg     1920

acacagccug ggauuucgga aguguaggcg gugcgcugaa cagccuuggg aaaggcaucc     1980

accagauuuu cggagccgcu uuuaaguccu uguucggcgg gaugucuugg uuuucgcaaa     2040

uccugauugg aacucuccug auguggcuug gccugaauac caagaacggu agcauauccu     2100

ugaugugucu cgcauuaggg ggagugcuga ucuuccuguc aacggccguu agugcuugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6138
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6138
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uuaacgccag aaaggagaaa aagaggcggg gcgcugacac cagcgugggg auagucggac      120

ugcuccuuac aacugcaaug gccgcggaag uuacgcgccg agguuccgcu uacuauaugu      180

accuggaucg uaaugacgcc ggcgaggcaa ucucuuuccc caccacauug gggaugaaca      240

aaugcuauau ucagaucaug gauuuaggac acauguguga cgccacuaug ucauacgaau      300

gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc aauaccacaa      360

guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggcuaga aggagccggc      420

gcgcagugac acuaccgucc cauucuacca gaaagcucca aacgaggucg cagacuuggc      480

uggagucacg ggaauacacc aagcaccuua uucgcguuga gaacuggauc uuucgaaauc      540

ccggauucgc ccuggcugcc gcggcaauag ccugguugcu cggcagcagu acaucccaga      600

aagugaucua ucuggucaug auucugcuca ucgcuccugc cuacucuauu agaugcaucg      660

gcgugagcaa cagggauuuu guagaaggga uguccggagg uacuuggguu gacauagugu      720

uagagcaugg cggguguguc accgugaugg cacaggauaa gccaacagug gacauugagc      780

uggucaccac gacuguuuca aauauggcug aagugcggag uuacugcuau gaggccagca      840

ucucugauau ggcguccgac ucacguuguc ccacacaagg agaagccuac cuugacaaac      900

agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg gguaacgggu      960

guggacuguu cggcaaaggc agucuaguca caugcgcaaa guuugcuugc ucuaagaaaa     1020

ugaccgggaa guccauucag ccugagaauc ucgaauacag gaucaugcug agcgugcacg     1080

gaucgcaaca cucugguaug aucguuaacg auacgggcca ugagaccgac gagaaucggg     1140

ccaaagugga aauuacacca aacucacccc gagcugaggc cacucuuggg ggauucggcu     1200

cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug uauuacuuaa     1260

caaugaauaa caagcacugg cugguccaua aagagugguu ccacgacaua ccacucccgu     1320

ggcacgcagg cgccgauacu ggaacacccc auuggaauaa caaggaagcu cuuguggagu     1380

uuaaagacgc gcacgcaaag agacagaccg uaguugugcu gggcagccag gagggggccg     1440

uccauaccgc uuuggccgga gcacuagaag ccgagaugga uggugcuaag ggcaggcugu     1500

ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag ggagugagcu     1560

auucauugug uacugccgca uucacguuua caaaaauccc ugcggaaacc cuccauggca     1620

cugucaccgu ggagguucag uacgcuggua cagacgggcc augcaaggug cccgcccaaa     1680

uggcaguaga uaugcagacc cugacuccug ucggcagauu aauuacagcc aacccaguga     1740

ucacggaaag uaccgaaaau uccaagauga ugcuggagcu agaucccccg uucggagaca     1800

gcuacauagu uauuggcgug ggggagaaaa agaucacuca ccacuggcau aggucuggau     1860

caacaaucgg uaaggcuuuu gaggccaccg uccguggcgc aaaacggaug gcugugcucg     1920

gggacacagc cugggauuuc ggaaguguag gcggugcgcu gaacagccuu gggaaaggca     1980

uucaccagau cuucggagcc gcuuuuaagu ccuuguucgg cgggaugucu ugguuuucgc     2040

aaauucugau aggaacucuc cugauguggc uuggccugaa uaccaagaac gguagcaucu     2100

ccuugaugug ucucgcauua gggggagugc ugauuuuccu gucaacggcc guuagugcuu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6139
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6139
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaaaaagagg cggggcgcug acaccagcgu ggggaucguc ggacugcucc      120

uuacaacugc aauggccgcg gaaguuacgc gccgagguuc cgcuuacuau auguaccugg      180

aucguaauga cgccggcgag gcaauuucuu uccccaccac auuggggaug aacaaaugcu      240

auauacagau cauggauuua ggacacaugu gugacgccac uaugucauac gaaugcccua      300

ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaauacc acaaguaccu      360

ggguggucua ugguacuugu caucacaaga aaggggaggc uagaaggagc cggcgcgcag      420

ugacacuacc gucccauucu accagaaagc uccaaacgag gucgcagacu uggcuggagu      480

cacgggaaua caccaagcac cuuauucgcg uugagaacug gaucuuucga aaucccggau      540

ucgcccuggc ugccgcggca auugccuggu ugcucggcag caguacaucc cagaaaguga      600

ucuaucuggu caugauacug cucaucgcuc cugccuacuc uauuagaugc aucggcguga      660

gcaacaggga uuuuguagaa gggauguccg gagguacuug gguugacauu guguuagagc      720

auggcgggug ugucaccgug auggcacagg auaagccaac aguggacauc gagcugguca      780

ccacgacugu uucaaauaug gcugaagugc ggaguuacug cuaugaggcc agcauaucug      840

auauggcguc cgacucacgu ugucccacac aaggagaagc cuaccuugac aaacagagcg      900

auacccagua uguaugcaag cgcacuuugg uggacagagg cugggguaac ggguguggac      960

uguucggcaa aggcagucua gucacaugcg caaaguuugc uugcucuaag aaaaugaccg     1020

ggaaguccau ucagccugag aaucucgaau acaggaucau gcugagcgug cacggaucgc     1080

aacacucugg uaugauuguu aacgauacgg gccaugagac cgacgagaau cgggccaaag     1140

uggaaaucac accaaacuca ccccgagcug aggccacucu ugggggauuc ggcucccugg     1200

guuuggauug ugaaccucgc accgggcucg acuuuaguga ucuguauuac uuaacaauga     1260

auaacaagca cuggcugguc cauaaagagu gguuccacga caucccacuc ccguggcacg     1320

caggcgccga uacuggaaca ccccauugga auaacaagga agcucuugug gaguuuaaag     1380

acgcgcacgc aaagagacag accguaguug ugcugggcag ccaggagggg gccguccaua     1440

ccgcuuuggc cggagcacua gaagccgaga uggauggugc uaagggcagg cuguccucug     1500

ggcaccucaa augccggcug aagauggaca aacuucgccu gaagggagug agcuauucau     1560

uguguacugc cgcauucacg uuuacaaaaa uuccugcgga aacccuccau ggcacuguca     1620

ccguggaggu ucaguacgcu gguacagacg ggccaugcaa ggugcccgcc caaauggcag     1680

uagauaugca gacccugacu ccugucggca gauuaauaac agccaaccca gugaucacgg     1740

aaaguaccga aaauuccaag augaugcugg agcuagaucc cccguucgga gacagcuaca     1800

uuguuaucgg cgugggggag aaaaagauaa cucaccacug gcauaggucu ggaucaacaa     1860

uugguaaggc uuuugaggcc accguccgug gcgcaaaacg gauggcugug cucggggaca     1920

cagccuggga uuucggaagu guaggcggug cgcugaacag ccuugggaaa ggcauccacc     1980

agaucuucgg agccgcuuuu aaguccuugu ucggcgggau gucuugguuu ucgcaaauuc     2040

ugaucggaac ucuccugaug uggcuuggcc ugaauaccaa gaacgguagc auuuccuuga     2100

ugugucucgc auuaggggga gugcugauau uccugucaac ggccguuagu gcuugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6140
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6140
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa aaagaggcgg ggcgcugaca ccagcguggg gaucgucgga cugcuccuua      120

caacugcaau ggccgcggaa guuacgcgcc gagguuccgc uuacuauaug uaccuggauc      180

guaacgacgc cggcgaggca auuucuuucc ccaccacauu ggggaugaau aaaugcuaua      240

uacagaucau ggauuuagga cacaugugug acgccacuau gucauacgaa ugcccuaugc      300

uggaugaggg cguggaacca gacgauguag acuguuggug caacaccaca aguaccuggg      360

uggucuaugg uacuugucau cacaagaaag gggaggcuag aaggagccgg cgcgcaguga      420

cacuaccguc ccauucuacc agaaagcucc aaacgagguc gcagacuugg cuggagucac      480

gggaauacac caagcaccuu auucgcguug agaauuggau cuuucgaaac cccggauucg      540

cccuggcugc cgcggcaauu gccugguugc ucggcagcag uacaucccag aaagugaucu      600

aucuggucau gauacugcuc aucgcuccug ccuacucuau uagaugcauc ggcgugagca      660

auagggauuu uguagaaggg auguccggag guacuugggu ugacauugug uuagagcaug      720

gcgggugugu caccgugaug gcacaggaua agccaacagu ggacaucgag cuggucacca      780

cgacuguuuc aaacauggcu gaagugcgga guuacugcua ugaggccagc auaucugaua      840

uggcguccga cucacguugu cccacacaag gagaagccua ccuugacaaa cagagcgaua      900

cccaguaugu augcaagcgc acuuuggugg acagaggcug ggguaauggg uguggacugu      960

ucggcaaagg cagucuaguc acaugcgcaa aguuugcuug cucuaagaaa augaccggga     1020

aguccauuca gccugagaac cucgaauaca ggaucaugcu gagcgugcac ggaucgcaac     1080

acucugguau gauuguuaau gauacgggcc augagaccga cgagaaccgg gccaaagugg     1140

aaaucacacc aaauucaccc cgagcugagg ccacucuugg gggauucggc ucccuggguu     1200

uggauuguga accucgcacc gggcucgacu uuagugaucu guauuacuua acaaugaaca     1260

auaagcacug gcugguccau aaagaguggu uccacgacau cccacucccg uggcacgcag     1320

gcgccgauac uggaacaccc cauuggaaca auaaggaagc ucuuguggag uuuaaagacg     1380

cgcacgcaaa gagacagacc guaguugugc ugggcagcca ggagggggcc guccauaccg     1440

cuuuggccgg agcacuagaa gccgagaugg auggugcuaa gggcaggcug uccucugggc     1500

accucaaaug ccggcugaag auggacaaac uucgccugaa gggagugagc uauucauugu     1560

guacugccgc auucacguuu acaaaaauuc cugcggaaac ccuccauggc acugucaccg     1620

uggagguuca guacgcuggu acagacgggc caugcaaggu gcccgcccaa auggcaguag     1680

auaugcagac ccugacuccu gucggcagau uaauaacagc caacccagug aucacggaaa     1740

guaccgagaa cuccaagaug augcuggagc uagauccccc guucggagac agcuacauug     1800

uuaucggcgu gggggagaaa aagauaacuc accacuggca uaggucugga ucaacaauug     1860

guaaggcuuu ugaagccacc guccguggcg caaaacggau ggcugugcuc ggggacacag     1920

ccugggauuu cggaagugua ggcggugcgc ugaauagccu ugggaaaggc auccaccaga     1980

ucuucggagc cgcuuuuaag uccuuguucg gcgggauguc uugguuuucg caaauucuga     2040

ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu uccuugaugu     2100

gucucgcauu agggggagug cugauauucc ugucaacggc cguuagugcu ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6141
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6141
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaaaaa gaggcggggc gccgacacca gcguggggau cgucggacug cuccuuacaa      120

cugcuauggc agccgaaguu acgcgccgag guuccgcgua cuauauguac cuggaucgua      180

acgacgcugg cgaggccauu ucuuucccca ccacauuggg gaugaauaaa ugcuauauac      240

agaucaugga uuuaggacac augugugacg caacuauguc auacgaaugc ccuaugcugg      300

augagggcgu ggaaccagac gauguagacu guuggugcaa caccacaagu accugggugg      360

ucuaugguac uugucaucac aagaaagggg aggccagaag gagccggcgc gcugugacac      420

uaccguccca uucuaccaga aagcuccaaa cgaggucgca gacuuggcug gagucacggg      480

aauacaccaa gcaccuuauu cgcguugaga auuggaucuu ucgaaacccc ggauucgcac      540

uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaaa gugaucuauc      600

uggucaugau acugcucauc gccccugcuu acucuauuag augcaucggc gugagcaaua      660

gggauuuugu agaagggaug uccggaggua cuuggguuga cauuguguua gagcauggcg      720

ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug gucaccacga      780

cuguuucaaa cauggcagaa gugcggaguu acugcuauga ggcuagcaua ucugauaugg      840

ccuccgacuc acguuguccc acacaaggag aagcguaccu ugacaaacag agcgauaccc      900

aguauguaug caagcgcacu uugguggaca gaggcugggg uaaugggugu ggacuguucg      960

gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaagaaaaug accgggaagu     1020

ccauucagcc ugagaaccuc gaauacagga ucaugcugag cgugcacgga ucgcaacacu     1080

cugguaugau uguuaaugau acgggccaug agaccgacga gaaccgggcu aaaguggaaa     1140

ucacaccaaa uucaccccga gccgaggcua cucuuggggg auucggcucc cuggguuugg     1200

auugugaacc ucgcaccggg cucgacuuua gugaucugua uuacuuaaca augaacaaua     1260

agcacuggcu gguccauaaa gagugguucc acgacauccc acucccgugg cacgccggcg     1320

cagauacugg aacaccccau uggaacaaua aggaagcccu uguggaguuu aaagacgcuc     1380

acgcgaagag acagaccgua guugugcugg gcagccagga gggggcaguc cauaccgccu     1440

uggcuggagc ccuagaagca gagauggaug gugccaaggg caggcugucc ucugggcacc     1500

ucaaaugccg gcugaagaug gacaaacuuc gccugaaggg agugagcuau ucauugugua     1560

cugcugccuu cacguuuaca aaaauuccug cagaaacccu ccauggcacu gucaccgugg     1620

agguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug gccguagaua     1680

ugcagacccu gacuccuguc ggcagauuaa uaacagcaaa cccagugauc acggaaagua     1740

ccgagaacuc caagaugaug cuggagcuag aucccccguu cggagacagc uacauuguua     1800

ucggcguggg ggagaaaaag auaacucacc acuggcauag gucuggauca acaauuggua     1860

aagccuuuga agcuaccguc cguggcgcca agcggauggc agugcucggg gacacagcuu     1920

gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc caccagaucu     1980

ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa auucugaucg     2040

gaacucuccu gauguggcuu ggccugaaca ccaagaaugg uagcauuucc uugauguguc     2100

ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6142
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6142
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaaaaagag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc cuuacaacug      120

cuauggcagc cgaaguuacg cggcgcgguu ccgcguacua uauguaccug gaucgaaacg      180

acgcuggcga ggccauuucu uuccccacca cauuggggau gaauaaaugc uauauacaga      240

ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgaaugcccu augcuggaug      300

agggcgugga accagacgau guagacuguu ggugcaacac cacaaguacc uggguggucu      360

augguacuug ucaucacaag aaaggggagg cccguagaag caggcgggcu gugacacuac      420

cgucccauuc uacccgcaag cuccaaacga gaucgcagac uuggcuggag ucaagggaau      480

acaccaagca ccuuauucgg guugagaauu ggaucuuucg caaccccgga uucgcacugg      540

ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug aucuaucugg      600

ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug agcaauagag      660

auuuuguaga agggaugucc ggagguacuu ggguugacau uguguuagag cauggcgggu      720

gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc accacgacug      780

uuucaaacau ggcagaagug aggaguuacu gcuaugaggc uagcauaucu gauauggccu      840

ccgacucacg gugucccaca caaggagaag cguaccuuga caaacagagc gauacccagu      900

auguaugcaa gcguacuuug guggaccgcg gcugggguaa ugggugugga cuguucggca      960

aaggcagucu agucacaugc gccaaguuug caugcucuaa gaaaaugacc gggaagucca     1020

uucagccuga gaaccucgaa uacagaauca ugcugagcgu gcacggaucg caacacucug     1080

guaugauugu uaaugauacg ggccaugaga ccgacgagaa cagggcuaaa guggaaauca     1140

caccaaauuc accccgggcc gaggcuacuc uugggggauu cggcucccug gguuuggauu     1200

gugaaccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug aacaauaagc     1260

acuggcuggu ccauaaagag ugguuccacg acaucccacu cccguggcac gccggcgcag     1320

auacuggaac accccauugg aacaauaagg aagcccuugu ggaguuuaaa gacgcucacg     1380

cgaagcgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau accgccuugg     1440

cuggagcccu agaagcagag auggauggug ccaagggcag acuguccucu gggcaccuca     1500

aaugcaggcu gaagauggac aaacuucggc ugaagggagu gagcuauuca uuguguacug     1560

cugccuucac guuuacaaaa auuccugcag aaacccucca uggcacuguc accguggagg     1620

uucaguacgc ggguacagac gggccaugca aggugcccgc ucaaauggcc guagauaugc     1680

agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg gaaaguaccg     1740

agaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac auuguuaucg     1800

gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca auugguaaag     1860

ccuuugaagc uaccgucagg ggcgccaagc guauggcagu gcucggggac acagcuuggg     1920

auuucggaag uguaggcggu gcccugaaua gccuugggaa aggcauccac cagaucuucg     1980

gagcggccuu uaaguccuug uucggcggga ugucuugguu uucgcaaauu cugaucggaa     2040

cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug augugucucg     2100

cuuuaggggg agugcugaua uuccugucaa cggcaguuag ugccugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6143
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6143
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagaag gggcgccgac accagcgugg ggaucgucgg acugcuccuu acaacugcua      120

uggcagccga aguuacgcgg cgcgguuccg cguacuauau guaccuggau cgaaacgacg      180

cuggcgaggc cauuucuuuc cccaccacau uggggaugaa uaagugcuau auacagauca      240

uggauuuagg acacaugugu gacgcaacua ugucauacga augcccuaug cuggaugagg      300

gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg guggucuaug      360

guacuuguca ucacaaaaag ggggaggccc guagaagcag gcgggcugug acacuaccgu      420

cccauucuac ccgcaaacuc caaacgagau cgcagacuug gcuggaguca agggaauaca      480

ccaagcaccu uauucggguu gagaauugga ucuuucgcaa ccccggauuc gcacuggccg      540

cugccgcgau ugcaugguug cucggcagca guacauccca gaaggugauc uaucugguca      600

ugauacugcu caucgccccu gcuuacucua uucgaugcau cggcgugagc aauagagauu      660

uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau ggcgggugug      720

ucaccgugau ggcccaggau aaaccaacag uggacaucga gcuggucacc acgacuguuu      780

caaacauggc agaagugagg aguuacugcu augaggcuag cauaucugau auggccuccg      840

acucacggug ucccacacaa ggagaagcgu accuugacaa gcagagcgau acccaguaug      900

uaugcaaacg uacuuuggug gaccgcggcu gggguaaugg guguggacug uucggcaagg      960

gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa gaugaccggg aaauccauuc     1020

agccugagaa ccucgaauac agaaucaugc ugagcgugca cggaucgcaa cacucuggua     1080

ugauuguuaa ugauacgggc caugagaccg acgagaacag ggcuaaggug gaaaucacac     1140

caaauucacc ccgggccgag gcuacucuug ggggauucgg cucccugggu uuggauugug     1200

aaccucgaac cgggcucgac uuuagugauc uguauuacuu aacaaugaac aauaaacacu     1260

ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgcc ggcgcagaua     1320

cuggaacacc ccauuggaac aauaaagaag cccuugugga guuuaaggac gcucacgcga     1380

aacgccagac cguaguugug cugggcagcc aggagggggc aguccauacc gccuuggcug     1440

gagcccuaga agcagagaug gauggugcca agggcagacu guccucuggg caccucaagu     1500

gcaggcugaa aauggacaag cugcggcuga aaggagugag cuauucauug uguacugcug     1560

ccuucacguu uacaaagauu ccugcagaaa cccuccaugg cacugucacc guggagguuc     1620

aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua gauaugcaga     1680

cccugacucc ugucggccgc uuaauaacag caaacccagu gaucacggaa aguaccgaga     1740

acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu guuaucggcg     1800

ugggggaaaa gaaaauaacu caccacuggc auagaucugg aucaacaauu gguaaggccu     1860

uugaggcuac cgucaggggc gccaaacgua uggcagugcu cggggacaca gcuugggauu     1920

ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag aucuucggag     1980

cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug aucggaacuc     2040

uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug ugucucgcuc     2100

uugggggagu guuaauauuc cugucaacgg caguuagugc cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6144
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6144
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagaagggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca acugcuaugg      120

cagccgaggu uacgcggcgc gguuccgcgu acuauaugua ccuggaucga aacgacgcug      180

gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua cagaucaugg      240

auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug gaugaaggcg      300

uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug gucuauggua      360

cuugucauca caaaaagggg gaagcccgua gaagcaggcg ggcugugaca cuaccguccc      420

auucuacccg caaacuccaa acgagaucgc agacuuggcu ggagucaagg gaguacacca      480

agcaccuuau ucggguugaa aauuggaucu uucgcaaccc cggauucgca cuggccgcug      540

ccgcgauugc augguugcuc ggcagcagua caucccagaa ggugaucuau cuggucauga      600

uacugcucau cgccccugcu uacucuauuc gaugcaucgg cgugagcaau agagauuuug      660

uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc ggguguguca      720

ccgugauggc ccaggauaaa ccaacagugg acaucgagcu ggucaccacg acuguuucaa      780

acauggcaga ggugaggagu uacugcuaug aagcuagcau aucugauaug gccuccgacu      840

cacggugucc cacacaagga gaggcguacc uugacaagca gagcgauacc caguauguau      900

gcaaacguac uuugguggac cgcggcuggg guaaugggug uggacuguuc ggcaagggca      960

gucuagucac augcgccaaa uuugcaugcu cuaagaagau gaccgggaaa uccauucagc     1020

cugaaaaccu cgaguacaga aucaugcuga gcgugcacgg aucgcaacac ucugguauga     1080

uuguuaauga uacgggccau gaaaccgacg agaacagggc uaagguggag aucacaccaa     1140

auucaccccg ggccgaagcu acucuugggg gauucggcuc ccuggguuug gauugugagc     1200

cucgaaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau aaacacuggc     1260

ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc gcagauacug     1320

gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu cacgcgaaac     1380

gccagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc uuggcuggag     1440

cccuagaggc agaaauggau ggugccaagg gcagacuguc cucugggcac cucaagugca     1500

ggcugaaaau ggacaagcug cggcugaaag gagugagcua uucauugugu acugcugccu     1560

ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug gaaguucagu     1620

acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau augcagaccc     1680

ugacuccugu cggccgcuua auaacagcaa acccagugau cacggagagu accgaaaacu     1740

ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu aucggcgugg     1800

gggagaagaa aauaacucac cacuggcaua gaucuggauc aacaauuggu aaggccuuug     1860

aagcuaccgu caggggcgcc aaacguaugg cagugcucgg ggacacagcu ugggauuucg     1920

gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc uucggagcgg     1980

ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc ggaacucucc     2040

ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu cucgcucuug     2100

ggggaguguu aauauuccug ucaacggcag uuagugccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6145
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6145
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gaaggggcgc cgacaccagc guggggaucg ucggacugcu ccuuacaacu gcuauggcag      120

ccgagguuac gcggcgcggu uccgcguacu auauguaccu ggaucgaaac gacgcuggcg      180

aagccauuuc uuuccccacc acauugggga ugaauaaaug cuauauacag aucauggauu      240

uaggacacau gugugacgca acuaugucau acgagugccc uaugcuggau gaaggcgugg      300

agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc uaugguacuu      360

gucaucacaa gaaaggggaa gcccguagaa gcaggcgggc ugugacacua ccgucccauu      420

cuacccgcaa gcuccaaacg agaucgcaga cuuggcugga gucaagggag uacaccaaac      480

accuuauucg gguugaaaau uggaucuuuc gcaaccccgg auucgcacug gccgcugccg      540

cgauugcaug guugcucggc agcaguacau cccagaaggu gaucuaucug gucaugauac      600

ugcucaucgc cccugcuuac ucuauucgau gcaucggcgu gagcaauaga gauuuuguag      660

aggggauguc cggagguacu uggguugaca uuguguuaga acauggcggg ugugucaccg      720

ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu guuucaaaca      780

uggcagaggu gaggaguuac ugcuaugaag cuagcauauc ugauauggcc uccgacucac      840

ggugucccac acaaggagag gcguaccuug acaaacagag cgauacccag uauguaugca      900

agcguacuuu gguggaccgc ggcuggggua augggugugg acuguucggc aaaggcaguc      960

uagucacaug cgccaaguuu gcaugcucua aaaagaugac cgggaagucc auucagccug     1020

aaaaccucga guacagaauc augcugagcg ugcacggauc gcaacacucu gguaugauug     1080

uuaaugauac gggccaugaa accgacgaga acagggcuaa aguggagauc acaccaaauu     1140

caccccgggc cgaagcuacu cuugggggau ucggcucccu ggguuuggau ugugagccuc     1200

gaaccgggcu cgacuuuagu gaucuguauu acuuaacaau gaacaauaag cacuggcugg     1260

uccauaaaga augguuccac gacaucccac ucccguggca cgccggcgca gauacuggaa     1320

caccccauug gaacaauaag gaggcccuug uggaauuuaa agacgcucac gcgaagcgcc     1380

agaccguagu ugugcugggc agccaggagg gggcagucca uaccgccuug gcuggagccc     1440

uagaggcaga aauggauggu gccaaaggca gacuguccuc ugggcaccuc aagugcaggc     1500

ugaagaugga caaacuucgg cugaagggag ugagcuauuc auuguguacu gcugccuuca     1560

cguuuacaaa aauuccugca gagacccucc auggcacugu caccguggaa guucaguacg     1620

cggguacaga cgggccaugc aaggugcccg cucaaauggc cguagauaug cagacccuga     1680

cuccugucgg ccgcuuaaua acagcaaacc cagugaucac ggagaguacc gaaaacucca     1740

aaaugaugcu ggagcuagau cccccguucg gagacagcua cauuguuauc ggcguggggg     1800

agaagaagau aacucaccac uggcauagau cuggaucaac aauugguaaa gccuuugaag     1860

cuaccgucag gggcgccaag cguauggcag ugcucgggga cacagcuugg gauuucggaa     1920

guguaggcgg ugcccugaau agccuuggga aaggcaucca ccagaucuuc ggagcggccu     1980

uuaaguccuu guucggcggg augucuuggu uuucgcaaau ucugaucgga acucuccuga     2040

uguggcuugg ccugaacacc aaaaauggua gcauuuccuu gaugugucuc gcuuuagggg     2100

gagugcugau auuccuguca acggcaguua gugccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6146
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6146
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ggggcgccga caccagcgug gggaucgucg gacugcuccu uacaacugcu auggcagccg      120

agguuacgcg gcgcgguucc gcguacuaua uguaccugga ucgaaacgac gcuggcgaag      180

ccauuucuuu ccccaccaca uuggggauga auaagugcua uauacagauc auggauuuag      240

gacacaugug ugacgcaacu augucauacg agugcccuau gcuggaugaa ggcguggagc      300

cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau gguacuuguc      360

aucacaaaaa gggggaagcc cguagaagca ggcgggcugu gacacuaccg ucccauucua      420

cccgcaaacu ccaaacgaga ucgcagacuu ggcuggaguc aagggaguac accaagcacc      480

uuauucgggu ugaaaauugg aucuuucgca accccggauu cgcacuggcc gcugccgcga      540

uugcaugguu gcucggcagc aguacauccc agaaagugau cuaucugguc augauacugc      600

ucaucgcccc ugcuuacucu auucgaugca ucggcgugag caauagagau uuuguagagg      660

ggauguccgg agguacuugg guugacauug uguuagaaca uggcgggugu gucaccguga      720

uggcccagga uaagccaaca guggacaucg agcuggucac cacgacuguu ucaaacaugg      780

cagaggugag gaguuacugc uaugaagcua gcauaucuga uauggccucc gacucacggu      840

gucccacaca aggagaggcg uaccuugaca agcagagcga uacccaguau guaugcaaac      900

guacuuuggu ggaccgcggc ugggguaaug gguguggacu guucggcaag ggcagucuag      960

ucacaugcgc caaauuugca ugcucuaaga aaaugaccgg gaaguccauu cagccugaaa     1020

accucgagua cagaaucaug cugagcgugc acggaucgca acacucuggu augauuguua     1080

augauacggg ccaugaaacc gacgagaaca gggcuaaggu ggagaucaca ccaaauucac     1140

cccgggccga agcuacucuu gggggauucg gcucccuggg uuuggauugu gagccucgaa     1200

ccgggcucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac uggcuggucc     1260

auaaggaaug guuccacgac aucccacucc cguggcacgc cggcgcagau acuggaacac     1320

cccauuggaa caauaaagag gcccuugugg aauuuaagga cgcucacgcg aaacgccaga     1380

ccguaguugu gcugggcagc caggaggggg caguccauac cgccuuggcu ggagcccuag     1440

aggcagaaau ggauggugcc aagggcagac uguccucugg gcaccucaaa ugcaggcuga     1500

agauggacaa gcugcggcug aaaggaguga gcuauucauu guguacugcu gccuucacgu     1560

uuacaaagau uccugcagag acccuccaug gcacugucac cguggaaguu caguacgcgg     1620

guacagacgg gccaugcaaa gugcccgcuc aaauggccgu agauaugcag acccugacuc     1680

cugucggccg cuuaauaaca gcaaacccag ugaucacgga gaguaccgaa aacuccaaga     1740

ugaugcugga gcuagauccc ccguucggag acagcuacau uguuaucggc gugggggaga     1800

aaaagauaac ucaccacugg cauagaucug gaucaacaau ugguaaggcc uuugaagcua     1860

ccgucagggg cgccaaacgu auggcagugc ucggggacac agcuugggau uucggaagug     1920

uaggcggugc ccugaauagc cuugggaagg gcauccacca gaucuucgga gcggccuuua     1980

aauccuuguu cggcgggaug ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu     2040

ggcuuggccu gaacaccaag aaugguagca uuuccuugau gugucucgcu cuugggggag     2100

uguuaauauu ccugucaacg gcaguuagug ccugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6147
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6147
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug gcagccgagg      120

uuacgaggcg ggguuccgcg uacuauaugu accuggaucg caacgacgcu ggcgaagcca      180

uuucuuuccc caccacauug gggaugaaua agugcuauau acagaucaug gauuuaggac      240

acauguguga cgcaacuaug ucauacgagu gcccuaugcu ggaugaaggc guggagccag      300

acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu acuugucauc      360

acaaaaaggg ggaagcccga cguagcagaa gggcugugac acuaccgucc cauucuaccc      420

ggaaacucca aacgcgcucg cagacuuggc uggagucaag agaguacacc aagcaccuua      480

uuaggguuga aaauuggauc uuucggaacc ccggauucgc acuggccgcu gccgcgauug      540

caugguugcu cggcagcagu acaucccaga aagugaucua ucuggucaug auacugcuca      600

ucgccccugc uuacucuauu cgcugcaucg gcgugagcaa ucgagauuuu guagagggga      660

uguccggagg uacuuggguu gacauugugu uagaacaugg cggguguguc accgugaugg      720

cccaggauaa gccaacagug gacaucgagc uggucaccac gacuguuuca aacauggcag      780

aggugagaag uuacugcuau gaagcuagca uaucugauau ggccuccgac ucaagguguc      840

ccacacaagg agaggcguac cuugacaagc agagcgauac ccaguaugua ugcaaacgga      900

cuuuggugga ccguggcugg gguaaugggu guggacuguu cggcaagggc agucuaguca      960

caugcgccaa auuugcaugc ucuaagaaaa ugaccgggaa guccauucag ccugaaaacc     1020

ucgaguaccg caucaugcug agcgugcacg gaucgcaaca cucugguaug auuguuaaug     1080

auacgggcca ugaaaccgac gagaacagag cuaaggugga gaucacacca aauuccccca     1140

gggccgaagc uacucuuggg ggauucggcu cacuggguuu ggauugugag ccucggaccg     1200

ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaaacacugg cugguccaua     1260

aggaaugguu ccacgacauc ccacucccgu ggcacgccgg cgcagauacu ggaacacccc     1320

auuggaacaa uaaagaggcc cuuguggaau uuaaggacgc ucacgcgaaa cgacagaccg     1380

uaguugugcu gggcagccag gagggggcag uccauaccgc cuuggcugga gcccuagagg     1440

cagaaaugga uggugccaag ggccgccugu ccucugggca ccucaaaugc agacugaaga     1500

uggacaagcu gaggcugaaa ggagugagcu auucauugug uacugcugcc uucacguuua     1560

caaagauucc ugcagagacc cuccauggca cugucaccgu ggaaguucag uacgcgggua     1620

cagacgggcc augcaaagug cccgcucaaa uggccguaga uaugcagacc cugacuccug     1680

ucggccgguu aauaacagca aacccaguga ucacggagag uaccgaaaac uccaagauga     1740

ugcuggagcu agaucccccg uucggagaca gcuacauugu uaucggcgug ggggagaaaa     1800

agauaacuca ccacuggcau cgcucuggau caacaauugg uaaggccuuu gaagcuaccg     1860

ucagaggcgc caaaaggaug gcagugcucg gggacacagc uugggauuuc ggaaguguag     1920

gcggugcccu gaauagccuu gggaagggca uccaccagau cuucggagcg gccuuuaaau     1980

ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc cugauguggc     2040

uuggccugaa caccaagaau gguagcauuu ccuugaugug ucucgcucuu gggggagugu     2100

uaauauuccu gucaacggca guuagugccu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6148
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6148
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gucggacugc uccuuacaac ugcuauggca gccgagguua      120

cgagaagggg uuccgcguac uauauguacc uggaucggaa cgacgcuggc gaagccauuu      180

cuuuccccac cacauugggg augaauaagu gcuauauaca gaucauggau uuaggacaca      240

ugugugacgc aacuauguca uacgagugcc cuaugcugga ugaaggcgug gagccagacg      300

auguagacug uuggugcaac accacaagua ccuggguggu cuaugguacu ugucaucaca      360

aaaaggggga agcccgccga agccguagag cugugacacu accgucccau ucuaccagga      420

aacuccaaac gcggucgcag acuuggcugg agucacgcga guacaccaag caccuuauua      480

gaguugaaaa uuggaucuuu aggaaccccg gauucgcacu ggccgcugcc gcgauugcau      540

gguugcucgg cagcaguaca ucccagaaag ugaucuaucu ggucaugaua cugcucaucg      600

ccccugcuua cucuauucgg ugcaucggcg ugagcaaucg cgauuuugua gaggggaugu      660

ccggagguac uuggguugac auuguguuag aacauggcgg gugugucacc gugauggccc      720

aggauaagcc aacaguggac aucgagcugg ucaccacgac uguuucaaac auggcagagg      780

ugcgaaguua cugcuaugaa gcuagcauau cugauauggc cuccgacuca agauguccca      840

cacaaggaga ggcguaccuu gacaagcaga gcgauaccca guauguaugc aaaaggacuu      900

ugguggaccg gggcuggggu aaugggugug gacuguucgg caagggcagu cuagucacau      960

gcgccaaauu ugcaugcucu aagaaaauga ccgggaaguc cauucagccu gaaaaccucg     1020

aguaccguau caugcugagc gugcacggau cgcaacacuc ugguaugauu guuaaugaua     1080

cgggccauga aaccgacgag aaccgcgcua agguggagau cacaccaaau ucccccagag     1140

ccgaagcuac ucuuggggga uucggcucac uggguuugga uugugagccu aggaccgggc     1200

ucgacuuuag ugaucuguau uacuuaacaa ugaacaauaa acacuggcug guccauaagg     1260

aaugguucca cgacauccca cucccguggc acgccggcgc agauacugga acaccccauu     1320

ggaacaauaa agaggcccuu guggaauuua aggacgcuca cgcgaaacgg cagaccguag     1380

uugugcuggg cagccaggag ggggcagucc auaccgccuu ggcuggagcc cuagaggcag     1440

aaauggaugg ugccaagggc cgacuguccu cugggcaccu caaaugccgc cugaagaugg     1500

acaagcugag acugaaagga gugagcuauu cauuguguac ugcugccuuc acguuuacaa     1560

agauuccugc agagacccuc cauggcacug ucaccgugga aguucaguac gcggguacag     1620

acgggccaug caaagugccc gcucaaaugg ccguagauau gcagacccug acuccugucg     1680

gcagguuaau aacagcaaac ccagugauca cggagaguac cgaaaacucc aagaugaugc     1740

uggagcuaga ucccccguuc ggagacagcu acauuguuau cggcgugggg gagaaaaaga     1800

uaacucacca cuggcaucgg ucuggaucaa caauugguaa ggccuuugaa gcuaccgucc     1860

gcggcgccaa aagaauggca gugcucgggg acacagcuug ggauuucgga aguguaggcg     1920

gugcccugaa uagccuuggg aagggcaucc accagaucuu cggagcggcc uuuaaauccu     1980

uguucggcgg gaugucuugg uuuucgcaaa uucugaucgg aacucuccug auguggcuug     2040

gccugaacac caagaauggu agcauuuccu ugaugugucu cgcucuuggg ggaguguuaa     2100

uauuccuguc aacggcaguu agugccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6149
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6149
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucguc gggcugcucc uuacaacugc uauggcagcc gagguuacga      120

gaaggggauc cgcguacuau auguaccugg aucggaacga cgcuggugaa gccauuucuu      180

uccccaccac auugggcaug aauaagugcu auauacagau cauggauuua gggcacaugu      240

gugacgcaac uaugucauac gagugcccua ugcuggauga aggaguggag ccagacgaug      300

uagacuguug gugcaacacc acaaguaccu ggguggucua uggcacuugu caucacaaaa      360

agggugaagc ccgccgaagc cguagagcug ugacacuacc gucccauucu accaggaaac      420

uccaaacgcg gucgcagacu uggcuggagu cacgcgagua caccaagcac cuuauuagag      480

uugaaaauug gaucuuuagg aaccccgggu ucgcacuggc cgcugccgcg auugcauggu      540

ugcucggaag caguacaucc cagaaaguga ucuaucuggu caugauacug cucaucgccc      600

cugcuuacuc uauucggugc aucggcguga gcaaucgcga uuuuguagag ggcauguccg      660

ggggaacuug gguugacauu guguuagaac augguggcug ugucaccgug auggcccagg      720

auaagccaac aguggacauc gagcugguca ccacgacugu uucaaacaug gcagaggugc      780

gaaguuacug cuaugaagcu agcauaucug auauggccuc cgacucaaga ugucccacac      840

aaggggaggc guaccuugac aagcagagcg auacccagua uguaugcaaa aggacuuugg      900

uggaccgggg auggggcaau gguugugggc uguucggaaa gggcagucua gucacaugcg      960

ccaaauuugc augcucuaag aaaaugaccg gcaaguccau ucagccugaa aaccucgagu     1020

accguaucau gcugagcgug cacgggucgc aacacucugg aaugauuguu aaugauacgg     1080

gucaugaaac cgacgagaac cgcgcuaagg uggagaucac accaaauucc cccagagccg     1140

aagcuacucu uggcggguuc ggaucacugg gcuuggauug ugagccuagg accggucucg     1200

acuuuaguga ucuguauuac uuaacaauga acaauaaaca cuggcugguc cauaaggaau     1260

gguuccacga caucccacuc ccguggcacg ccggggcaga uacuggcaca ccccauugga     1320

acaauaaaga ggcccuugug gaauuuaagg acgcucacgc gaaacggcag accguaguug     1380

ugcugggaag ccaggagggc gcaguccaua ccgccuuggc uggggcccua gaggcagaaa     1440

uggauggagc caagggucga cuguccucug gccaccucaa augccgccug aagauggaca     1500

aacuuagacu gaagggggug agcuauucau uguguacugc ugccuucacg uuuacaaaga     1560

uuccugcaga gacccuccau ggaacuguca ccguggaagu ucaguacgcg ggcacagacg     1620

guccaugcaa agugcccgcu caaauggccg uagauaugca gacccugacu ccugucggga     1680

gguuaauaac agcaaaccca gugaucacgg agaguaccga aaacuccaag augaugcugg     1740

agcuagaucc cccguucggc gacagcuaca uuguuaucgg agugggcgag aaaaagauaa     1800

cucaccacug gcaucggucu gggucaacaa uuggaaaggc cuuugaagcu accguccgcg     1860

gugccaaaag aauggcagug cucggcgaca cagcuuggga uuucgggagu guaggaggcg     1920

cccugaauag ccuugguaag gggauccacc agaucuucgg cgcggccuuu aaauccuugu     1980

ucggaggcau gucuugguuu ucgcaaauuc ugaucgggac ucuccugaug uggcuuggac     2040

ugaacaccaa gaauggcagc auuuccuuga ugugucucgc ucuggguggg guguuaauau     2100

uccugucaac ggcaguuagu gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  6150
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6150
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgucggg cugcuccuua caacugccau ggcugcagag guuacgagaa      120

ggggauccgc cuacuauaug uaccuggauc ggaacgacgc gggugaagcu auuucuuucc      180

ccaccacauu gggcaugaau aagugcuaua uacagaucau ggauuuaggg cacaugugug      240

acgccacuau gucauacgag ugcccuaugc uggaugaagg aguggagcca gacgauguag      300

acuguuggug caacaccaca aguaccuggg uggucuaugg cacuugucau cacaaaaagg      360

gugaagcacg ccgaagccgu agagccguga cacuaccguc ccauucuacc aggaaacucc      420

aaacgcgguc gcagacuugg cuggagucac gcgaguacac caagcaccuu auuagaguug      480

aaaauuggau cuuuaggaac cccggguucg cucuggcagc cgcugccauu gcgugguugc      540

ucggaagcag uacaucccag aaagugaucu aucuggucau gauacugcuc aucgcacccg      600

ccuacucuau ucggugcauc ggcgugagca aucgcgauuu uguagagggc auguccgggg      660

gaacuugggu ugacauugug uuagaacaug guggcugugu caccgugaug gcucaggaua      720

agccaacagu ggacaucgag cuggucacca cgacuguuuc aaacauggcc gaggugcgaa      780

guuacugcua ugaagcaagc auaucugaua uggcuuccga cucaagaugu cccacacaag      840

gggaggccua ccuugacaag cagagcgaua cccaguaugu augcaaaagg acuuuggugg      900

accggggaug gggcaauggu ugugggcugu ucggaaaggg cagucuaguc acaugcgcga      960

aauuugccug cucuaagaaa augaccggca aguccauuca gccugaaaac cucgaguacc     1020

guaucaugcu gagcgugcac gggucgcaac acucuggaau gauuguuaau gauacggguc     1080

augaaaccga cgagaaccgc gcaaaggugg agaucacacc aaauuccccc agagcugaag     1140

ccacucuugg cggguucgga ucacugggcu uggauuguga gccuaggacc ggucucgacu     1200

uuagugaucu guauuacuua acaaugaaca auaaacacug gcugguccau aaggaauggu     1260

uccacgacau cccacucccg uggcacgcug gggccgauac uggcaccccu cauuggaaca     1320

auaaagaggc acuuguggaa uuuaaggacg cccacgcuaa acggcagaca guaguugugc     1380

ugggaagcca ggagggcgcg guccauaccg cauuggccgg ggcucuagag gccgaaaugg     1440

auggagcaaa gggucgacug uccucuggcc accucaaaug ccgccugaag auggacaaac     1500

uuagacugaa gggggugagc uauucauugu guacugccgc uuucacguuu acaaagauuc     1560

cugccgagac ccuccaugga acugucaccg uggaaguuca guacgcaggc acagacgguc     1620

caugcaaagu gcccgcgcaa auggcuguag auaugcagac ccugacuccu gucgggaggu     1680

uaauaacagc caacccagug aucacggaga guaccgaaaa cuccaagaug augcuggagc     1740

uagauccccc guucggcgac agcuacauug uuaucggagu gggcgagaaa aagauaacuc     1800

accacuggca ucggucuggg ucaacaauug gaaaggcauu ugaagccacc guccgcggug     1860

cuaaaagaau ggccgugcuc ggcgacacag caugggauuu cgggagugua ggaggcgcuc     1920

ugaauagccu ugguaagggg auccaccaga ucuucggcgc cgcguuuaaa uccuuguucg     1980

gaggcauguc uugguuuucg caaauucuga ucgggacucu ccugaugugg cuuggacuga     2040

acaccaagaa uggcagcauu uccuugaugu gucucgcccu ggguggggug uuaauauucc     2100

ugucaacggc uguuagugca ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6151
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  6151
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6152
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6152
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagccguu acgagaaggg      120

gauccgcgua cuauauguac cuggaccgga acgaugcugg ugaggccauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

caacuauguc auacgaaugc ccuaugcugg acgagggagu ggaaccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aggcccgccg aagccguaga gcugugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gaaucacgcg aguacaccaa gcaccuuauu agaguugaga      480

auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcuu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agaaggcaug uccgggggaa      660

cuuggguuga uauuguguua gagcauggug gcugugucac cgugauggcc caggacaagc      720

caacagugga uaucgaacug gucaccacga cuguuucaaa cauggcagag gugcgaaguu      780

acugcuauga ggcuagcaua ucugacaugg ccuccgauuc aagauguccc acacaagggg      840

aagcguaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgccaaau      960

uugcaugcuc uaagaaaaug accggcaagu ccauucagcc ugagaaccuc gaauaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

agaccgauga aaaccgcgcu aagguggaga ucacaccaaa uucccccaga gccgaggcua     1140

cucuuggcgg guucggauca cugggcuugg acugugaacc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gagugguucc     1260

acgauauccc acucccgugg cacgccgggg cagacacugg cacaccccau uggaacaaua     1320

aagaagcccu uguggaguuu aaggaugcuc acgcgaaacg gcagaccgua guugugcugg     1380

gaagccagga aggcgcaguc cauaccgccu uggcuggggc ccuagaggca gagauggacg     1440

gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca aagauuccug     1560

cagaaacccu ccauggaacu gucaccgugg agguucagua cgcgggcaca gacgguccau     1620

gcaaagugcc cgcucaaaug gccguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagcaaa cccagugauc acggaaagua ccgagaacuc caagaugaug cuggaacuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggccuuuga ggcuaccguc cgcggugcca     1860

aaagaauggc agugcucggc gauacagcuu gggacuucgg gaguguagga ggcgcccuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua auauuccugu     2100

caacggcagu uagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6153
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6153
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccuuac aacugcuaug gcagccgcgg      120

uuacgaggcg ggguuccgcu uacuauaugu accuggaucg caacgacgcc ggcgaggcaa      180

uuucuuuccc caccacauug gggaugaaua agugcuauau acagaucaug gauuuaggac      240

acauguguga cgccacuaug ucauacgaau gcccuaugcu ggaugagggc guggaaccag      300

acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggu acuugucauc      360

acaaaaaggg ggaggcucga cguagcagaa gggcagugac acuaccgucc cauucuaccc      420

ggaaacucca aacgcgcucg cagacuuggc uggaaucaag agaguacacc aagcaccuua      480

uuaggguuga gaauuggauc uuucggaacc ccggauucgc ccuggcugcc gcggcaauug      540

ccugguugcu cggcagcagu acaucccaga aagugaucua ucuggucaug auacugcuca      600

ucgcuccugc cuacucuauu cgcugcaucg gcgugagcaa ucgagauuuu guagaaggga      660

uguccggagg uacuuggguu gacauugugu uagagcaugg cggguguguc accgugaugg      720

cacaggauaa gccaacagug gacaucgaac uggucaccac gacuguuuca aacauggcug      780

aggugagaag uuacugcuau gaggccagca uaucugauau ggcguccgac ucaagguguc      840

ccacacaagg agaagccuac cuugacaagc agagcgauac ccaguaugua ugcaaacgga      900

cuuuggugga ccguggcugg gguaaugggu guggacuguu cggcaagggc agucuaguca      960

caugcgcaaa auuugcuugc ucuaagaaaa ugaccgggaa guccauucag ccugagaacc     1020

ucgaauaccg caucaugcug agcgugcacg gaucgcaaca cucugguaug auuguuaaug     1080

auacgggcca ugagaccgac gaaaacagag ccaaggugga gaucacacca aauuccccca     1140

gggcugaggc cacucuuggg ggauucggcu cacuggguuu ggauugugaa ccucggaccg     1200

ggcucgacuu uagugaucug uauuacuuaa caaugaacaa uaaacacugg cugguccaua     1260

aggagugguu ccacgacauc ccacucccgu ggcacgcagg cgccgauacu ggaacacccc     1320

auuggaacaa uaaagaagcu cuuguggagu uuaaggacgc gcacgcaaaa cgacagaccg     1380

uaguugugcu gggcagccag gaaggggccg uccauaccgc uuuggccgga gcacuagagg     1440

ccgagaugga uggugcuaag ggccgccugu ccucugggca ccucaaaugc agacugaaga     1500

uggacaagcu gaggcugaaa ggagugagcu auucauugug uacugccgca uucacguuua     1560

caaagauucc ugcggaaacc cuccauggca cugucaccgu ggagguucag uacgcuggua     1620

cagacgggcc augcaaagug cccgcccaaa uggcaguaga uaugcagacc cugacuccug     1680

ucggccgguu aauaacagcc aacccaguga ucacggaaag uaccgagaac uccaagauga     1740

ugcuggaacu agaucccccg uucggagaca gcuacauugu uaucggcgug ggggagaaaa     1800

agauaacuca ccacuggcau cgcucuggau caacaauugg uaaggcuuuu gaggccaccg     1860

ucagaggcgc aaaaaggaug gcugugcucg gggacacagc cugggauuuc ggaaguguag     1920

gcggugcgcu gaauagccuu gggaagggca uccaccagau cuucggagcc gcuuuuaaau     1980

ccuuguucgg cgggaugucu ugguuuucgc aaauucugau cggaacucuc cugauguggc     2040

uuggccugaa caccaagaau gguagcauuu ccuugaugug ucucgcacuu gggggagugu     2100

uaauauuccu gucaacggcc guuagugcuu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6154
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6154
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc caggaagcgg cgcucucacg acgugcucac cguccaguuc cuuauccugg      120

ggauguuguu aaugacagga gguguuacuc gacguggcuc agccuacuau auguaccugg      180

auagaaacga cgcuggggag gcaauuaguu uucccacgac ccuaggaaug aauaaaugcu      240

auauacaaau cauggaucuc ggccauaugu gugacgccac aaugagcuac gaaugcccua      300

ugcuggauga ggguguggaa ccagacgaug uagacuguug gugcaacacu accuccacau      360

ggguggucua ugggaccugu caccauaaga aaggagaggc gaggcggucu cgcagagcug      420

ugacucuucc gucgcacuca acaaggaagc ugcagacccg gagccagacg ugguuggaaa      480

gucgcgagua cacuaaacau cucauucgag uugagaauug gaucuucaga aaccccggcu      540

uugcccuggc agccgcugca auugccuggc ugcucggcuc cucuaccagc cagaagguga      600

ucuauuuagu caugauacug cuuaucgcuc cugccuacuc cauuaggugc aucggggugu      660

caaaucggga uuucguagaa ggaaugagug guggcacaug gguugacauu guguuggagc      720

acgggggaug ugucacugug auggcgcaag auaagccaac cguggacauc gaacugguca      780

caaccacggu uagcaacaug gcagaggugc guucuuacug cuaugaggcc uccauaucag      840

auauggcuag cgacagucgc ugucccacuc agggcgaagc cuaccuagac aaacagucug      900

auacacagua uguaugcaag agaacccucg uggacagggg uugggggaau ggauguggcc      960

uguuuggcaa agggucccuu gucacuugcg caaaguucgc uugcagcaaa aagaugacag     1020

gaaagucgau ucaaccugag aaccuggaau accggaucau guugucugug cacgguuccc     1080

agcauucagg caugauuguu aaugauaccg ggcacgagac ggacgaaaac cgagccaaag     1140

uggagaucac cccaaauagu ccccgcgcgg aggccacacu cggaggcuuu gguagccugg     1200

gguuagauug ugaaccuaga acuggccugg acuucuccga ucucuauuac cuuaccauga     1260

acaauaagca uuggcugguc cacaaagagu gguuucacga caucccauug ccguggcaug     1320

caggagcuga uacaggcacu ccccacugga acaauaagga agcccuagug gaguucaaag     1380

acgcucaugc caagaggcag accguaguug ugcugggguc ucaggaagga gcaguccaca     1440

cagcccucgc uggugcgcug gaggcagaga uggauggcgc caaagggcgg cuuagcucag     1500

gacaucugaa gugccgcuug aagauggaca aacucagacu gaagggcgug aguuauuccu     1560

uauguaccgc ugccuuuacu uucacgaaaa uuccugcaga aacacugcac gguaccguca     1620

cuguggaggu ucaauacgcc gggaccgacg gcccaugcaa ggugcccgcu cagauggccg     1680

uagauaugca gacacuaacc ccugucggaa ggcucauaac ugcaaaccca gugaucacag     1740

aaagcacgga gaacucuaaa augaugcugg aacuugaucc cccguuuggc gacucauaca     1800

uuguuaucgg ggugggagag aagaagauaa cccaccauug gcaccguagu gguagcacua     1860

uuggcaaagc guucgaggcu acaguccgag gggccaagcg gauggcagug uugggagaca     1920

ccgccuggga uuucggcucc guaggugggg cucucaauuc ucugggcaaa ggaauccauc     1980

aaaucuuugg cgccgcauuc aagucgcugu uugggggaau gagcugguuc ucccagauuc     2040

uuaucggcac acuguugaug uggcucgguc ugaacacuaa aaauggguca auuaguuuaa     2100

ugugucuggc ucuaggaggc gugcucauau uucugucuac cgccguuagc gcgugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6155
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6155
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc caggaagcgg cgcucucacg acgugcucac cguccaguuc cuuauccugg      120

ggauguuguu aaugacagga gccguuacuc gacgugguuc agcuuacuau auguaccugg      180

auagaaacga cgcaggcgag gccauuaguu uucccacgac ccuagggaug aauaaaugcu      240

auauacaaau cauggaucuc ggacauaugu gugacgcgac aaugagcuac gaaugcccua      300

ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacu accuccacau      360

ggguggucua ugguaccugu caccauaaga aaggggaggc uaggcggucu cgcagagccg      420

ugacucuucc gucgcacuca acaaggaagc ugcagacccg gagccagacg ugguuggaaa      480

gucgcgagua cacuaaacau cucauucgag uugagaauug gaucuucaga aaccccggau      540

uugcacuggc cgcugcagcc auugcuuggc ugcucggcuc cucuaccagc cagaagguga      600

ucuauuuagu caugauacug cuuaucgccc cugcguacuc cauuaggugc aucggcgugu      660

caaaucggga uuucguagaa gggaugagug gagguacaug gguugacauu guguuggagc      720

acggcgggug ugucacugug auggcacaag auaagccaac cguggacauc gaacugguca      780

caaccacggu uagcaacaug gccgaggugc guucuuacug cuaugaggcu uccauaucag      840

auauggccag cgacagucgc ugucccacuc agggagaagc auaccuagac aaacagucug      900

auacacagua uguaugcaag agaacccucg uggacagggg cugggguaau ggguguggac      960

uguuuggcaa aggcucccuu gucacuugcg cuaaguucgc cugcagcaaa aagaugacag     1020

ggaagucgau ucaaccugag aaccuggaau accggaucau guugucugug cacggauccc     1080

agcauucagg uaugauuguu aaugauaccg gccacgagac ggacgaaaac cgagcgaaag     1140

uggagaucac cccaaauagu ccccgcgccg aggcaacacu cgggggauuu ggcagccugg     1200

guuuagauug ugaaccuaga acugggcugg acuucuccga ucucuauuac cuuaccauga     1260

acaauaagca uuggcugguc cacaaagagu gguuucacga caucccauug ccguggcaug     1320

cuggcgccga uacaggaacu ccccacugga acaauaagga agcucuagug gaguucaaag     1380

acgcccaugc aaagaggcag accguaguug ugcugggcuc ucaggaaggg gccguccaca     1440

cagcucucgc gggagcacug gaggccgaga uggauggugc uaaaggccgg cuuagcucag     1500

ggcaucugaa gugccgcuug aagauggaca aacucagacu gaagggagug aguuauuccu     1560

uauguaccgc cgcauuuacu uucacgaaaa uuccugccga aacacugcac ggcaccguca     1620

cuguggaggu ucaauacgcu gguaccgacg ggccaugcaa ggugcccgcc cagauggcag     1680

uagauaugca gacacuaacc ccugucggca ggcucauaac ugcgaaccca gugaucacag     1740

aaagcacgga gaacucuaaa augaugcugg aacuugaucc cccguuugga gacucauaca     1800

uuguuaucgg cgugggggag aagaagauaa cccaccauug gcaccguagu ggaagcacua     1860

uugguaaggc uuucgaggcc acaguccggg gcgcaaaacg aauggccgug uugggggaca     1920

ccgcuuggga uuucggaucc guaggcggug cccucaauuc ucuggggaaa ggcauccauc     1980

aaaucuuugg agcagcuuuc aagucgcugu uuggcgggau gagcugguuc ucccagauuc     2040

uuaucggaac acuguugaug uggcucggcc ugaacacuaa aaaugguuca auuaguuuaa     2100

ugugucuggc ccuaggggga gugcucauau uucugucuac cgcgguuagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6156
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 2 opt

<400>  6156
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacauag      720

uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca guggacauug      780

agcuggucac cacgacuguu ucaaauaugg cugaagugcg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu ggaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuugugg     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguaguugu gcugggcagc caggaggggg     1440

ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggaa acccuccaug     1620

gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag acccugacuc cugucggcag guuaauuaca gccaacccag     1740

ugaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggacac agccugggau uucggaagug uaggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag ugcugauuuu ccugucaacg gccguuagug     2160

cugacgucgg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6157
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 2 opt

<400>  6157
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacacuug ugacgccacc augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacaa      360

ccaguacuug gguggucuau gguacauguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacccuaccg ucccauucua cgaggaagcu ccaaacucgg ucgcagaccu      480

ggcuggaguc acgcgaauac acaaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacuuccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguaccugg guugacgugg      720

ucuuagagca uggcgggugu gugacaguga uggcacagga uaagccaacc gucgacauag      780

agcugguuac gacuacagug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccaccca aggagaagcc uaccuugaca      900

aacagagcga uacucaguau gugugcaaga gaacauuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugaccugcgc aaaguuugcu ugcucuaaga     1020

aaaugacggg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauaccgg ccaugagaca gacgagaauc     1140

gagccaaagu cgaaaucacu ccaaacucac cccgcgcuga ggccacccuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa cagggcucga cuuuagugau cuguauuacu     1260

uaacuaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau accggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac ugcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacggcc gcauucacau uuaccaaaau accugcggaa acucuccaug     1620

gcaccgugac aguugaggug caguacgcug guaccgacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acucugacac cugugggcag guuaaucacg gccaacccag     1740

uuauuaccga aaguacugaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac acaccacugg caucguucug     1860

gaucaaccau cgguaaggcu uuugaggcca cagugcgggg cgcaaaacga auggcuguac     1920

ucggggacac ugccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacc cuccugaugu ggcuuggccu gaaugcaaag aacgguagca     2100

uauccuugau gugucucgcc uuagggggag uucugaucuu ccugucaacg gcugucagug     2160

ccgacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6158
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 2 opt

<400>  6158
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca uuggggguga      240

auaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu cacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuauaaaagu ggaaaauugg aucuuuagaa      540

acccgggauu cgcucugguu gccguggcga uugcaugguu gcucggcagu uccacaucuc      600

agaaggucau cuaccuggug augauucugc ucaucgcccc cgcuuauagc auaaggugca      660

uuggcguauc caaucgggau uuuguugagg ggaugucagg agguacuugg guggacgucg      720

uguuagaaca cggcgggugu gugaccguca uggcccagga uaaaccuaca guugacaucg      780

agcuggugac cacgacugua aguaacaugg cagaggugcg uagcuacugc uacgaggcuu      840

cuauuuccga uauggccuca gacagccgcu guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gucugcaaaa gaacuuuggu ggacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag uuacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauc cagcccgaaa accucgagua ucggaucaug cugucggugc     1080

acggaucuca acauuccggu augauuggcu acgaaaccga ugaggaccga gcaaaggucg     1140

aggugacacc uaauucacca cgcgcugaag ccacucuugg gggauucggc agucuggguu     1200

uggauuguga gcccagaacc gggcucgacu uuagcgaucu guauuacuua acaaugaaca     1260

auaaacacug gcugguacau aaggaauggu uccacgacau accucuccca uggcacgcug     1320

gcgccgauac uggaaccccg cauuggaaca auaaagaggc acuuguugaa uuuaaggacg     1380

cccacgcuaa gaggcagaca guggucgugc ugggcuccca ggagggggcg guccauaccg     1440

cauuggccgg agcucuagag gccgaaaugg auggugcaaa aggccggcug uucucugggc     1500

accucaagug ccgccugaaa auggacaaac uuagacugaa gggagugagc uauucauugu     1560

guacugccgc uuuuacguuc acaaagguuc ccgccgagac ccuccauggc acugugaccg     1620

uagaagucca guacgcaggu acagacgggc cuugcaagau cccagugcaa auggcgguug     1680

auaugcagac ccugacuccc gugggcaggu uaauuacagc uaacccuguc aucacggaga     1740

guaccgaaaa uuccaaaaug augcuggagc uagacccacc cuuuggagau agcuacauag     1800

ugauuggcgu aggggacaag aaaaucacuc accacuggca ucguucugga ucaacaaucg     1860

guaaggccuu cgaggcaacc gugcggggcg ccaaacgaau ggcuguucuc ggggauacag     1920

ccugggacuu cggaaguguc ggcggugugu uuaacagccu ggggaagggc auucaccaga     1980

ucuucggagc agcuuuuaag ucccuuuucg gcgggauguc uugguuuucg caaauuuuga     2040

uaggaacucu gcucgugugg cugggccuua acaccaaaaa ugguagcauc ucccugacgu     2100

guuuggcccu cgggggaguc augauuuucc ugucaaccgc gguuagugcc gaugugggcu     2160

gcucugugga cuuuagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6159
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 2 opt

<400>  6159
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6160
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 2 opt

<400>  6160
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca uuggggguga      240

auaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu cacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuauaaaagu ggaaaauugg aucuuuagaa      540

acccgggauu cgcucugguu gccguggcga uugcaugguu gcucggcagu uccacaucuc      600

agaaggucau cuaccuggug augauucugc ucaucgcccc cgcuuauagc auaaggugca      660

uuggcguauc caaucgggau uuuguugagg ggaugucagg agguacuugg guggacgucg      720

uguuagaaca cggcgggugu gugaccguca uggcccagga uaaaccuaca guugacaucg      780

agcuggugac cacgacugua aguaacaugg cagaggugcg uagcuacugc uacgaggcuu      840

cuauuuccga uauggccuca gacagccgcu guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gucugcaaaa gaacuuuggu ggacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag uuacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauc cagcccgaaa accucgagua ucggaucaug cugucggugc     1080

acggaucuca acauuccggu augauuguca augauaccgg cuacgaaaca gacgagaacc     1140

gagcaaaggu ggagguaacu ccuaauucac cacgcgcuga agccacccuu gggggauucg     1200

gcagucuggg uuuggauugu gagcccagaa cagggcucga cuuuagcgau cuguauuacu     1260

uaacuaugaa caauaaacac uggcugguuc auaaggaaug guuccacgac auaccucucc     1320

cauggcacgc uggcgccgau accggaacac cgcauuggaa caauaaagag gcacuugugg     1380

aauuuaagga cgcccacgcu aagaggcaga ccgucguggu ccugggcucc caggaggggg     1440

cggugcauac ugcauuggcc ggagcucuag aggccgaaau ggauggugca aaaggcaagc     1500

uguucucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug aagggaguua     1560

gcuauucauu guguacggcc gcuuuuacau ucaccaaggu gcccgccgag acucuccaug     1620

gcaccguaac agucgaagug caguacgcag guaccgacgg gccuugcaaa aucccaguuc     1680

aaauggcggu ggauaugcag acucugacac ccgucggcag auuaauuacg gcuaacccug     1740

ugaucaccga gaguacugaa aacuccaaga ugaugcugga gcuagaccca cccuuuggag     1800

auagcuacau aguaauuggc gugggggaca aaaagaucac acaccacugg cauaggucug     1860

gaucaaccau cgguaaagcc uucgaggcaa caguucgugg cgccaagcgg auggcugucc     1920

ucggggauac ugccugggac uucggaagug ugggcggugu guuuaauagc cuggggaagg     1980

gcauucacca gaucuucgga gcagcuuuua aaucccuuuu cggcgggaug ucuugguuuu     2040

cgcaaauuuu gauaggaacc cugcucgucu ggcugggccu uaacacgaag aaugguagca     2100

ucucccugac cuguuuggcc cucgggggag uuaugauuuu ccugucaaca gcggugagug     2160

ccgauguggg cugcucugua gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6161
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 2 opt

<400>  6161
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucgugg      120

gacugcuccu uacaacugca auggccgcgg aaauuacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caaucucauu cgccaccaca uuggggguca      240

auaaaugcca cguucagauu auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguggagc cugacgaugu agacuguugg ugcaacacca      360

caagcaccug gguggucuac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu gacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuaucaaagu ugaaaauugg auauuuagaa      540

acccgggauu cacacuggug gcugucgcca ucgcgugguu gcucggcagu uccacuucuc      600

agaaggugau uuaccuggua augauccugc ucauugcacc cgccuauagc aucaggugca      660

uaggcguuuc caaucgggau uuuguggagg ggaugucagg agguaccugg gucgacgugg      720

uguuagaaca cggcgggugu gucacaguua uggcucagga uaaaccuacc guggacauug      780

agcugguaac gacuacagug aguaacaugg ccgagguccg uagcuacugc uacgaagcau      840

cuaucuccga uauggcuuca gacagccgcu guccaaccca aggagaggcc uaucuugaca      900

agcagaguga uacucaguac gugugcaaaa gaacauuggu ugacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag ugaccugcgc gaaguuuacg ugcuccaaaa     1020

agaugaccgg gaaaagcauu cagcccgaaa accucgagua ucggaucaug cugucggucc     1080

acggaucuca acauuccggu augaucguga augaugaaaa ccgagccaag guagagguua     1140

caccuaauuc accacgcgca gaggcuacuc uuggcggguu cggaagucug ggcuuggacu     1200

gugaacccag aaccggucuc gauuuuagcg accuguacua uuuaacaaug aacaauaaac     1260

acuggcuggu gcauaaggag ugguuccacg auauuccucu cccauggcac gccggggcug     1320

acacuggcac cccgcauugg aacaauaaag aagcccuugu cgaguuuaag gaugcacacg     1380

ccaagaggca gacagugguc gugcugggau cccaggaagg cgcuguucau accgcguugg     1440

caggggcccu agaggcugag auggacggag ccaaaggucg gcuguucucu ggccaccuca     1500

agugccgccu gaaaauggau aaacuuagac ugaagggggu gagcuacuca uuguguacug     1560

cagccuuuac guucacaaag guacccgcug aaacccucca uggaacuguc accguggagg     1620

uucaguaugc cggcacagac gguccuugca aggugccagc acaaauggcg gucgacaugc     1680

agacccugac ucccgugggg agguuaauaa cagcuaaccc uguaaucacg gaaaguaccg     1740

agaacuccaa aaugaugcug gaacuagauc cacccuuugg cgacagcuac auugugaucg     1800

gaguuggcga uaagaaaaua acucaccacu ggcaucguuc ugggucaaca auuggaaagg     1860

ccuucgaggc aaccguccgg ggugccaaac gaauggcugu gcucggcgac acagccuggg     1920

auuucgggag ugugggaggc gucuuuaaua gccuggguaa ggggauccac cagaucuucg     1980

gcgcagcuuu uaagucccuu uucggaggca ugucuugguu uucgcaaauu uugaucggga     2040

cucugcucgu uuggcuggga cuuaacacca aaaauggcag cauuucccug acguguuugg     2100

cccucggugg ggugaugaua uuccugucaa ccgcggugag ugccgacgua ggaugcucug     2160

ucgauuuuag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6162
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 2 opt

<400>  6162
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca uuggggguga      240

auaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accggaagaa aggggaagca cgcagaucca      420

ggcgggccgu cacacuacca ucucauucga cccgcaagcu ccaaacgcga ucacagacuu      480

ggcuggagag cagagaguau accaagcacc uuauaaaagu ggaaaauugg aucuuuagga      540

acccgggauu cgcucugguu gccguggcga uugcaugguu gcucggcagu uccacaucuc      600

agaaggucau cuaccuggug augauucugc ucaucgcccc cgcuuauagc auacggugca      660

uuggcguauc caaucgugau uuuguugagg ggaugucagg agguacuugg guggacgucg      720

uguuagaaca uggcgggugu gugaccguca uggcccagga uaaaccuaca guugacaucg      780

agcuggugac cacgacugua aguaacaugg cagaggugcg cagcuacugc uacgaggcuu      840

cuauuuccga uauggccuca gacagcagau guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gucugcaaaa ggacuuuggu ggaccggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag uuacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauc cagcccgaaa accucgagua ucgaaucaug cugucggugc     1080

acggaucuca acacuccggu augauuguca augauaccgg cuacgaaaca gacgagaacc     1140

gcgcaaaggu ggagguaacu ccuaauucac caagagcuga agccacccuu gggggauucg     1200

gcagucuggg uuuggauugu gagcccagga cagggcucga cuuuagcgau cuguauuacu     1260

uaacuaugaa caauaaacau uggcugguuc acaaggaaug guuccaugac auaccucucc     1320

cauggcacgc uggcgccgau accggaacac cgcacuggaa caauaaagag gcacuugugg     1380

aauuuaagga cgcccaugcu aagcggcaga ccgucguggu ccugggcucc caggaggggg     1440

cggugcacac ugcauuggcc ggagcucuag aggccgaaau ggauggugca aaaggcaagc     1500

uguucucugg gcaucucaaa ugccgccuga agauggacaa acuuagacug aagggaguua     1560

gcuauucauu guguacggcc gcuuuuacau ucaccaaggu gcccgccgag acucuccacg     1620

gcaccguaac agucgaagug caguacgcag guaccgacgg gccuugcaaa aucccaguuc     1680

aaauggcggu ggauaugcag acucugacac ccgucggcag guuaauuacg gcuaacccug     1740

ugaucaccga gaguacugaa aacuccaaga ugaugcugga gcuagaccca cccuuuggag     1800

auagcuacau aguaauuggc gugggggaca aaaagaucac acaucacugg caccguucug     1860

gaucaaccau cgguaaagcc uucgaggcaa caguucgggg cgccaagcga auggcugucc     1920

ucggggauac ugccugggac uucggaagug ugggcggugu guuuaauagc cuggggaagg     1980

gcauucauca gaucuucgga gcagcuuuua aaucccuuuu cggcgggaug ucuugguuuu     2040

cgcaaauuuu gauaggaacc cugcucgucu ggcugggccu uaacacgaag aaugguagca     2100

ucucccugac cuguuuggcc cucgggggag uuaugauuuu ccugucaaca gcggugagug     2160

ccgauguggg cugcucugua gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6163
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 2 opt

<400>  6163
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca uuggggguga      240

auaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu cacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuauaaaagu ggaaaauugg aucuuuagaa      540

acccgggauu cgcucugguu gccguggcga uugcaugguu gcucggcagu uccacaucuc      600

agaaggucau cuaccuggug augauucugc ucaucgcccc cgcuuauagc auaaggugca      660

uuggcguauc caaucgggau uuuguugagg ggaugucagg agguacuugg guggacgucg      720

uguuagaaca cggcgggugu gugaccguca uggcccagga uaaaccuaca guugacaucg      780

agcuggugac cacgacugua aguaacaugg cagaggugcg uagcuacugc uacgaggcuu      840

cuauuuccga uauggccuca gacagccgcu guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gucugcaaaa gaacuuuggu ggacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag uuacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauc cagcccgaaa accucgagua ucggaucaug cugucggugc     1080

acggaucuca acauuccggu augaccguca augauauugg cuacgaaaca gacgagaacc     1140

gagcaaaggu ggagguaacu ccuaauucac cacgcgcuga agccacccuu gggggauucg     1200

gcagucuggg uuuggauugu gagcccagaa cagggcucga cuuuagcgau cuguauuacu     1260

uaacuaugaa caauaaacac uggcugguuc auaaggaaug guuccacgac auaccucucc     1320

cauggcacgc uggcgccgau accggaacac cgcauuggaa caauaaagag gcacuugugg     1380

aauuuaagga cgcccacgcu aagaggcaga ccgucguggu ccugggcucc caggaggggg     1440

cggugcauac ugcauuggcc ggagcucuag aggccgaaau ggauggugca aaaggcaagc     1500

uguucucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug aagggaguua     1560

gcuauucauu guguacggcc gcuuuuacau ucaccaaggu gcccgccgag acucuccaug     1620

gcaccguaac agucgaagug caguacgcag guaccgacgg gccuugcaaa aucccaguuc     1680

aaauggcggu ggauaugcag acucugacac ccgucggcag auuaauuacg gcuaacccug     1740

ugaucaccga gaguacugaa aacuccaaga ugaugcugga gcuagaccca cccuuuggag     1800

auagcuacau aguaauuggc gugggggaca aaaagaucac acaccacugg cauaggucug     1860

gaucaaccau cgguaaagcc uucgaggcaa caguucgugg cgccaagcgg auggcugucc     1920

ucggggauac ugccugggac uucggaagug ugggcggugu guuuaauagc cuggggaagg     1980

gcauucacca gaucuucgga gcagcuuuua aaucccuuuu cggcgggaug ucuugguuuu     2040

cgcaaauuuu gauaggaacc cugcucgucu ggcugggccu uaacacgaag aaugguagca     2100

ucucccugac cuguuuggcc cucgggggag uuaugauuuu ccugucaaca gcggugagug     2160

ccgauguggg cugcucugua gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6164
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 2 opt

<400>  6164
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca uuggggguga      240

auaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu cacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuauaaaagu ggaaaauugg aucuuuagaa      540

acccgggauu cgcucugguu gccguggcga uugcaugguu gcucggcagu uccacaucuc      600

agaaggucau cuaccuggug augauucugc ucaucgcccc cgcuuauagc auaaggugca      660

uuggcguauc caaucgggau uuuguugagg ggaugucagg agguacuugg guggacgucg      720

uguuagaaca cggcgggugu gugaccguca uggcccagga uaaaccuaca guugacaucg      780

agcuggugac cacgacugua aguaacaugg cagaggugcg uagcuacugc uacgaggcuu      840

cuauuuccga uauggccuca gacagccgcu guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gucugcaaaa gaacuuuggu ggacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag uuacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauc cagcccgaaa accucgagua ucggaucaug cugucggugc     1080

acggaucuca acauuccggu augauuggcu acgaaaccga ugagaaucga gcaaaggucg     1140

aggugacacc uaacucacca cgcgcugaag ccacucuugg gggauucggc agucuggguu     1200

uggacuguga gcccagaacc gggcucgauu uuagcgaccu guauuacuua acaaugaaua     1260

acaaacacug gcugguacau aaggaauggu uccacgauau accucuccca uggcacgcug     1320

gcgccgacac uggaaccccg cauuggaaua acaaagaggc acuuguugaa uuuaaggaug     1380

cccacgcuaa gaggcagaca guggucgugc ugggcuccca ggagggggcg guccauaccg     1440

cauuggccgg agcucuagag gccgaaaugg acggugcaaa aggcaagcug uucucugggc     1500

accucaaaug ccggcugaag auggauaaac uucgccugaa gggagugagc uauucauugu     1560

guacugccgc uuuuacguuc acaaagguuc ccgccgagac ccuccauggc acugugaccg     1620

uagaagucca guacgcaggu acagacgggc cuugcaaaau cccagugcaa auggcgguug     1680

acaugcagac ccugacuccc gugggcagau uaauuacagc uaauccuguc aucacggaga     1740

guaccgaaaa cuccaagaug augcuggagc uagauccacc cuuuggagac agcuacauag     1800

ugauuggcgu aggggauaaa aagaucacuc accacuggca uaggucugga ucaacaaucg     1860

guaaagccuu cgaggcaacc gugcguggcg ccaagcggau ggcuguucuc ggggacacag     1920

ccugggauuu cggaaguguc ggcggugugu uuaacagccu ggggaagggc auucaccaga     1980

ucuucggagc agcuuuuaaa ucccuuuucg gcgggauguc uugguuuucg caaauuuuga     2040

uaggaacucu gcucgugugg cugggccuua auaccaagaa cgguagcauc ucccugacgu     2100

guuuggcccu cgggggaguc augauuuucc ugucaaccgc gguuagugcc gacgugggcu     2160

gcucugugga uuuuagcaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6165
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 2 opt

<400>  6165
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggagag     1980

gcauccacca gauuuucgga gccgcuuuua aauccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6166
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 2 opt

<400>  6166
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga cgcaagcgug gggauagucg      120

gacugcuccu uaccacagcc auggcggcug aaguuacucg acgugguucc gccuacuaua      180

uguaccugga uagaaaugac gcaggcgagg ccaucucuuu ccccacgacc uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgcuaca augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacua      360

ccaguacaug gguggucuau gguaccuguc aucacaagaa aggggaggca aggcggagcc      420

gcagagccgu gacucuaccg ucccauucua caaggaagcu ccaaacccgg ucgcagacgu      480

ggcuggaguc acgcgaauac acuaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcucuggcc gcggcagcca uagcuugguu gcucggcagc aguaccuccc      600

agaaagugau cuaucugguc augauucugc ucaucgcccc ugcauacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacaugg guugacgugg      720

ucuuagagca uggcgggugu gugacuguga uggcucagga uaagccaacc gucgacauag      780

agcugguuac aaccacggug ucaaauaugg ccgaaguacg uaguuacugc uaugaggcga      840

gcauuucuga uauggccucc gacucacgcu gucccacuca aggagaagca uaccuugaca      900

aacagagcga uacacaguau gugugcaaga gaaccuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacuugcgc uaaguuugcc ugcucuaaga     1020

aaaugacagg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauaccgg ccaugagacg gacgagaauc     1140

gagcuaaagu cgaaaucacc ccaaacucac cccgcgccga ggcaacacuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa cugggcucga cuuuagugau cuguauuacu     1260

uaaccaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcugau acaggaacuc cccauuggaa uaacaaggaa gcgcuuguag     1380

aguuuaaaga cgcacacgcc aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

cugugcauac agccuuggca ggagcccuag aagcugagau ggauggugcc aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguaccgca gcguucacuu uuacgaaaau accugcugaa acacuccaug     1620

gcaccgugac uguugaggug caguacgccg guaccgacgg gccaugcaag guacccgcac     1680

aaauggccgu cgauaugcag acacugaccc cugugggcag guuaaucacu gcuaacccag     1740

uuauuacaga aaguacggaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ccaccacugg caucguucug     1860

gaucaacuau cgguaaagcc uuugaggcaa cagugcgggg cgcuaagcga auggccguac     1920

ucggggacac cgcgugggau uucggaagug ugggcggugc ccugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gcugcauuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaaca cuccugaugu ggcuuggccu gaauacuaag aacgguagca     2100

uauccuugau gugucucgcc uuagggggag uucugaucuu ccugucaacc gcugucagug     2160

ccgacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6167
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 2 opt

<400>  6167
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggacu ggccuuggcu gccgcggcaa uagccuggcu ccugggcagc aguacauccc      600

agaaagugau cuaucugguc augauucucu uaaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uucguagaag ggauguccgg agguacuugg guugacgugg      720

uccuggagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcuuguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uacuuggaca      900

aacagagcga uacccaguau gugugcaaga gaacucuggu cgacaggggc ugggguaacg      960

gguguggacu auuuggcaaa ggcagucucg ugacaugcgc aaaguucgcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucuggaaua ccggauuaug cuuagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucug ggaggguuug     1200

gcuccuuggg ucucgauugu gaaccuagaa ccgggcugga cuucagugau uuauauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuucacgac auuccacuuc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucugguag     1380

aguucaaaga cgcgcacgca aagaggcaga ccguuguggu cuugggcagc caggaggggg     1440

ccgugcauac cgcucuagcc ggagcacugg aagccgagau ggauggugcu aagggccggc     1500

ucuccucugg gcaccugaaa ugccgccuua agauggacaa acugagauug aagggaguca     1560

gcuauucacu cuguacugcc gcauuuacgu ucacaaaaau accugcggaa acccugcaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag accuuaacuc cugugggcag gcugaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcuaga gcucgauccc ccguuuggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uucgaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ugggggacac agccugggau uuuggaagug ugggcggugc gcuuaacagc uuggggaaag     1980

gcauccacca gauuuucgga gccgcuuuca agucccuguu uggcgggaug ucuugguucu     2040

cgcaaauccu cauuggaacu cugcuuaugu ggcugggcuu gaauaccaag aacgguagca     2100

uaucccucau gugucuggca uuagggggag uucugaucuu ucuaucaacg gccgucagug     2160

cugacguggg cugcucugug gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6168
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 2 opt

<400>  6168
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg uaucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccauca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6169
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 2 opt

<400>  6169
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaauucg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu ggaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuugugg     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguaguugu gcugggcagc caggaggggg     1440

ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggaa acccuccaug     1620

gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag acccugacuc cugucggcag guuaauuaca gccaacccag     1740

ugaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggacac agccugggau uucggaagug uaggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag ugcugauuuu ccugucaacg gccguuagug     2160

cugacgucgg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6170
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 2 opt

<400>  6170
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacuggccc     1680

aaauggcagu cgauaugcag accuuaacuc ccgugggcag gcugaucaca gccaacccug     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcuaga gcucgaccca cccuucggag     1800

auagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ugggggacac agccugggau uucggaagug ugggcggugc gcuuaacagc uuggggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu cauuggaacu cugcuuaugu ggcugggcuu gaauaccaag aacgguagca     2100

uaucccucau gugucuggca uuagggggag uucugaucuu ccuaucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6171
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 2 opt

<400>  6171
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuac ccuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacaa      360

ccaguacuug gguggucuau gguacauguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacccuaccg ucccauucua cgaggaagcu ccaaacucgg ucgcagaccu      480

ggcuggaguc acgcgaauac acaaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacuuccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguaccugg guugacgugg      720

ucuuagagca uggcgggugu gugacaguga uggcacagga uaagccaacc gucgacauag      780

agcugguuac gacuacagug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccaccca aggagaagcc uaccuugaca      900

aacagagcga uacucaguau gugugcaaga gaacauuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugaccugcgc aaaguuugcu ugcucuaaga     1020

aaaugacggg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauaccgg ccaugagaca gacgagaauc     1140

gagccaaagu cgaaaucacu ccaaacucac cccgcgcuga ggccacccuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa cagggcucga cuuuagugau cuguauuacu     1260

uaacuaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau accggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac ugcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacggcc gcauucacau uuaccaaaau accugcggaa acucuccaug     1620

gcaccgugac aguugaggug caguacgcug guaccgacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acucugacac cugugggcag guuaaucacg gccaacccag     1740

uuauuaccga aaguacugaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac acaccacugg caucguucug     1860

gaucaaccau cgguaaggcu uuugaggcca cagugcgggg cgcaaaacga auggcuguac     1920

ucggggacac ugccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacc cuccugaugu ggcuuggccu gaauacgaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacc gccgucagug     2160

cugacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6172
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 2 opt

<400>  6172
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacg gucgugagug     2160

ccgacguggg cugcucuguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6173
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 2 opt

<400>  6173
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caagugcuug gguggucuau gguaccuguc aucacaagaa aggggaggca aggcggagcc      420

gcagagccgu gacucuaccg ucccauucua caaggaagcu ccaaacccgg ccccagacgu      480

ggcuggaguc gcgcgaauac acuaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

auccuggauu cgcucuggcc gcggcagcca uagcuugguu gcucggcuca agcaccaguc      600

agaaagugau cuaucugguc augauucugc ucaucgcccc agcauacucc auuaggugca      660

ucggcguguc uaaccgggau uuuguagaag ggaugagcgg agguacaugg guugacgugg      720

ucuuagagca uggcgggugu gugacuguga uggcucagga uaagcccacc gucgacauag      780

agcugguuac aaccacggug uccaauaugg ccgaaguacg uucauacugc uaugaggcga      840

guauuagcga uauggccucu gacucccgcu guccuacuca aggagaagca uaccuugaca      900

aacagucaga uacacaguau gugugcaaga gaaccuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagccuag ugacuugcgc uaaguuugcc ugcaguaaga     1020

aaaugacagg gaagucuauc cagccagaga aucucgaaua ccggauuaug cuguccguuc     1080

acggaagcca acacucgggu augaucguga acgauaccgg ccaugagacg gacgagaauc     1140

gagcuaaagu cgaaaucacc cccaacucuc cucgcgccga ggcaacacuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccaagaa cugggcucga cuuuucagau cuguauuacu     1260

uaaccaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccgcucc     1320

ccuggcacgc cggcgcugau acaggaacuc cucauuggaa uaacaaggaa gcgcuuguag     1380

aguuuaaaga cgcacacgcc aagaggcaga ccguuguggu ccugggcagu caggaggggg     1440

cugugcauac agccuuggca ggagcccuag aagcugagau ggauggugcc aagggccggc     1500

ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cuuauagcuu guguaccgca gcguucacuu uuacgaaaau accagcugaa acacuccaug     1620

gcaccgugac uguugaggug caguacgccg guaccgacgg gcccugcaag guaccugcac     1680

aaauggccgu cgauaugcag acacugaccc cagugggcag guuaaucacu gcuaaccccg     1740

uuauuacaga aucaacggag aauaguaaga ugaugcugga gcuagacccg ccuuucggag     1800

auuccuacau cgugauaggc gucggggaga aaaagauuac ccaccacugg caucguagcg     1860

gaucuacuau cgguaaagcc uuugaagcaa cagugcgggg cgcuaagcga auggccguac     1920

ucggggacac cgcgugggau uucggaucag ugggcggugc ccugaacagu cuugggaaag     1980

gcauccacca gauuuucgga gcugcauuua agagcuuguu cggcgggaug uccugguuuu     2040

cucaaauccu gauuggaaca cuccugaugu ggcuuggccu gaauacuaag aacgguucga     2100

ucagcuugau gugucucgcc uuagggggag uucugauauu ccuguccacc gcugucucag     2160

ccgacguggg cugcagugug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6174
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 2 opt

<400>  6174
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacaucca      600

aaaaggugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaaaccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca gggagaagcc uaccuugaca      900

agcaaagcga uacccaguau gugugcaaaa gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaag ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaaa     1020

agaugaccgg gaaauccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca gcacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaggu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaaacac uggcuggugc auaaggagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaagaa gcucuuguag     1380

aguuuaagga cgcgcacgca aagaggcaaa ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aaaggccggc     1500

uguccucugg gcaccucaag ugccgccuga aaauggacaa gcugagacug aaaggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaagau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

agauggcagu cgauaugcaa acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaaa ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga agaaaauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggaagg     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcagauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaaa aacgguagca     2100

uauccuugau gugucucgca cuugggggag uuuuaaucuu ccugucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6175
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 2 opt

<400>  6175
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacaaggc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucc auucggugca      660

ucggcguguc uaaccgugau uuuguagaag ggaugagcgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug uccaauaugg cugaaguacg cucauacugc uaugaggcca      840

guauuagcga uauggcgucu gacuccagau gucccacaca aggagaagcc uaccuugaca      900

aacagucaga uacccaguau gugugcaaga ggacuuuggu cgaccggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagccuag ugacaugcgc aaaguuugcu ugcaguaaga     1020

aaaugaccgg gaagucuauc cagccugaga aucucgaaua ccgaauuaug cuguccguuc     1080

acggaagcca acacucgggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gcgccaaagu cgaaaucaca ccaaacucuc ccagagcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagga ccgggcucga cuuuucagau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagcggcaga ccguuguggu ccugggcagu caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccgcc     1500

ugagcuccgg gcaccucaaa ugcagacuga agauggacaa acuuaggcug aagggagucu     1560

cuuauagcuu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggccg uuuaaucaca gccaacccag     1740

uuauuacgga aucaaccgag aauaguaaga ugaugcugga gcuagauccc ccguucggag     1800

acuccuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucggagcg     1860

gaucuacaau cgguaaggcu uuugaagcca ccgugcgagg cgcaaaaaga auggcuguac     1920

ucggggacac agccugggau uucggaucag ugggcggugc gcugaacagu cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agagcuuguu cggcgggaug uccugguuuu     2040

cucaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguucga     2100

ucagcuugau gugucucgca uuagggggag uucugauauu ccuguccacg gccgucucag     2160

cugacguggg cugcagugug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6176
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 2 opt

<400>  6176
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcagcgcuga caccuccgug ggcauagucg      120

ggcugcuccu uacaacugca auggccgcgg aaguuacgcg acguggaucu gcuuacuaua      180

uguaccugga uagaaaugac gccggugagg caaucucauu ccccaccaca uugggcauga      240

acaaaugcua uauucagauc auggauuuag ggcacaugug ugacgccacu augaguuacg      300

aaugcccuau gcuggaugag ggaguggaac cagacgaugu agacuguugg ugcaauacca      360

caagcaccug gguggucuau ggcacuuguc aucacaagaa aggugaggcu aggcgguccc      420

gcagagcagu gacacuaccg ucucauucga ccaggaagcu ccaaacgcgg ucacagacuu      480

ggcuggagag ccgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggguu cgcccuggcu gccgcggcaa uagccugguu gcucggaagu uccacaucuc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacagc auuaggugca      660

ucggcguguc caaccgggau uuuguagaag gcaugucagg gggaacuugg guugacgugg      720

ucuuagagca ugguggcugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug aguaauaugg cugaaguacg uagcuacugc uaugaggccu      840

cuauuuccga uauggcguca gacagccgcu gucccacaca aggggaagcc uaccuugaca      900

aacagaguga uacccaguau gugugcaaga gaacuuuggu cgacagggga uggggcaacg      960

guugugggcu guucggaaaa ggcucucuag ugacaugcgc aaaguuugcu ugcuccaaga     1020

aaaugaccgg caagagcauc cagccugaga aucucgaaua ccggauuaug cugucgguuc     1080

acgggucuca acacuccgga augaucguga acgauacggg ucaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu ggcggguucg     1200

gaagucuggg cuuggauugu gaaccuagaa ccggucucga cuuuagcgau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggggccgau acuggcacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggaucc caggagggcg     1440

ccgugcauac cgcuuuggcc ggggcacuag aagccgagau ggauggagcu aagggucggc     1500

ugucuagcgg ccaccucaaa ugccgccuga agauggacaa acuuagacug aagggggucu     1560

cauauaguuu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gaacugugac cguugaggug caguacgcug gcacagacgg uccaugcaag guacccgccc     1680

aaauggcagu cgauacccag acucugacac cuguggggag guuaaucacg gccaacccag     1740

uuauuaccga auccacugag aauagcaaga ugaugcugga gcuagauccc ccguucggcg     1800

acucuuacau cgugauagga gucggcgaga aaaagauuac acaccacugg caucguucag     1860

ggaguaccau cggaaaggcu uuugaagcca cagugcgggg ugcaaaacga auggcuguac     1920

ucggcgacac ugccugggau uucgggagcg ugggaggcgc gcugaacucc cuugguaaag     1980

ggauccacca gauuuucggc gccgcuuuua agucuuuguu cggaggcaug ucgugguuua     2040

gccaaauccu gauugggacc cuccugaugu ggcuuggacu gaauacgaag aacggcucca     2100

uaucauugau gugucucgca cugggugggg uuuuaaucuu ccugaguacc gccgucucug     2160

cugacguggg augcagcgug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6177
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 2 opt

<400>  6177
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccaaugug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguagc gcuuacuaua      180

uguaccugga uagaaacgac gccggcgagg caaucuccuu ccccaccaca uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucuuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaacacca      360

caucaaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggaguc      420

gcagagcagu gacacuaccg agccauucca ccaggaagcu ccaaacgcgg ucucagacuu      480

ggcuggaguc gcgcgaauac accaagcacc uuauucgagu ugagaauugg aucuuuagaa      540

accccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcuca agcacaaguc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucc auuaggugca      660

ucggcguguc uaaucgggau uuuguagaag ggaugagcgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug uccaacaugg cugaaguacg uucauacugc uaugaggcca      840

guauuagcga uauggcgucu gacucccgcu gucccacaca aggagaagcc uaccuugaca      900

aacagucaga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcagccuag ugacaugcgc aaaguuugcu ugcaguaaga     1020

aaaugaccgg gaagucuauc cagccugaga accucgaaua ccggauuaug cuguccguuc     1080

acggaagcca acacucgggu augaucguga augauacggg ccaugagacc gacgagaacc     1140

gagccaaagu cgaaaucaca ccaaauucuc cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuucagau cuguauuacu     1260

uaacaaugaa caauaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa caacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagu caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cuuauagcuu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaaucaca gccaauccag     1740

uuauuacgga aucaaccgag aacaguaaga ugaugcugga gcuagauccc ccguucggag     1800

acuccuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguagcg     1860

gaucuacaau cgguaaggcu uuugaagcca ccgugcgggg cgcacgaaga auggcuguac     1920

ucggggacac agccugggau uucggaucag ugggcggugc gcugaauagu cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agagcuuguu cggcgggaug uccugguuuu     2040

cucaaauccu gauuggaacu cuccugaugu ggcuuggccu gaacaccaaa aaugguucga     2100

ucagcuugau gugucucgca uuagggggag uucugauauu ccuguccacg gccgucucag     2160

cugacguggg cugcagugug gauuuuucua agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6178
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 2 opt

<400>  6178
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga aaccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg agguuacgcg acgugguucc gcuuacuaua      180

uguaccugga cagaaaugau gccggcgaag caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggacuuag gacacaugug ugaugccacu augucauacg      300

agugcccuau gcuggacgaa ggcguggagc cagaugacgu agauuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaauc acgcgaguac accaagcacc uuauucgagu ugaaaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggac uuuguagagg ggauguccgg agguacuugg guugaugugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga caagccaaca gucgauauag      780

aacugguuac cacgacugug ucaaauaugg cugagguacg uaguuacugc uaugaagcca      840

gcauuucuga cauggcgucc gauucacgcu gucccacaca aggagaggcc uaccuugaca      900

aacagagcga cacccaguau gugugcaaga gaacuuuggu cgauaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaaa aucucgagua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgacacggg ccaugagacc gaugaaaauc     1140

gagccaaagu cgagaucaca ccaaacucac cccgcgcuga agccacucuu gggggauucg     1200

gcucccuggg uuuggacugu gagccuagaa ccgggcucga uuuuagugac cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagaaug guuccacgau auuccacucc     1320

cguggcacgc aggcgccgac acuggaacac cccauuggaa uaacaaggag gcucuuguag     1380

aguuuaaaga ugcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaagggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aggccgaaau ggacggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggauaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggag acccuccaug     1620

gcacugugac cguugaagug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgacaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga gaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaagcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggauac agccugggac uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacg gccgucagug     2160

cugauguggg cugcucugug gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6179
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 2 opt

<400>  6179
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu uuaaggugca      660

uuggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

uccuggagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacaucg      780

agcuuguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauaucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uacuuggaca      900

aacagagcga uacccaguau gugugcaaga gaacucuggu cgacaggggc ugggguaacg      960

gguguggacu auucggcaaa ggcagucucg ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucuggaaua ccggaucaug cuuagcguuc     1080

acggaucgca acacucuggu augauuguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucug ggaggguucg     1200

gcuccuuggg ucucgauugu gaaccuagaa ccgggcugga cuuuagugau uuauauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac aucccacuuc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucugguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu cuugggcagc caggaggggg     1440

ccgugcauac cgcucuagcc ggagcacugg aagccgagau ggauggugcu aagggccggc     1500

ucuccucugg gcaccugaaa ugccgccuua agauggacaa acugagauug aagggaguca     1560

gcuauucacu cuguacugcc gcauucacgu uuacaaaaau uccugcggaa acccugcaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag accuuaacuc cugugggcag gcugauaaca gccaacccag     1740

uuaucacgga aaguaccgaa aauuccaaga ugaugcuaga gcucgauccc ccguucggag     1800

acagcuacau ugugaucggc gucggggaga aaaagauaac ucaccacugg caucguucug     1860

gaucaacaau ugguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ugggggacac agccugggau uucggaagug ugggcggugc gcuuaacagc uuggggaaag     1980

gcauccacca gaucuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu caucggaacu cugcuuaugu ggcugggcuu gaauaccaag aacgguagca     2100

uuucccucau gugucuggca uuagggggag uucugauauu ccuaucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6180
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 2 opt

<400>  6180
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccaaugug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguagc gcuuacuaua      180

uguaccugga uagaaacgac gccggcgagg caaucuccuu ccccaccaca uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucuuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaacacca      360

caucaaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggaguc      420

gcagagcagu gacacuaccg agccauucca ccaggaagcu ccaaacgcgg ucucagacuu      480

ggcuggaguc gcgcgaauac accaagcacc uuauucgagu ugagaauugg auguuuagaa      540

accccggauu cgcccuggcu gccgcggcaa ucgccugguu gcucggcuca agcacaaguc      600

agaaagugau auaucugguc augauccugc ucauugcucc ugccuacucc aucaggugca      660

uuggcguguc uaaucgggau uuuguagaag ggaugagcgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacaucg      780

agcugguuac cacgacugug uccaacaugg cugaaguacg uucauacugc uaugaggcca      840

guauaagcga uauggcgucu gacucccgcu gucccacaca aggagaagcc uaccuugaca      900

aacagucaga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcagccuag ugacaugcgc aaaguuugcu ugcaguaaga     1020

aaaugaccgg gaagucuauu cagccugaga accucgaaua ccggaucaug cuguccguuc     1080

acggaagcca acacucgggu augauuguga augauacggg ccaugagacc gacgagaacc     1140

gagccaaagu cgaaaucaca ccaaauucuc cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuucagau cuguauuacu     1260

uaacaaugaa caauaagcac uggcuggugc auaaagagug guuccacgac aucccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa caacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagu caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cuuauagcuu guguacugcc gcauucacgu uuacaaaaau uccugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaauaaca gccaauccag     1740

uuaucacgga aucaaccgag aacaguaaga ugaugcugga gcuagauccc ccguucggag     1800

acuccuacau ugugaucggc gucggggaga aaaagauaac ucaccacugg caucguagcg     1860

gaucuacaau ugguaaggcu uuugaagcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggacac agccugggau uucggaucag ugggcggugc gcugaauagu cuugggaaag     1980

gcauccacca gaucuucgga gccgcuuuua agagcuuguu cggcgggaug uccugguuuu     2040

cucaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag aaugguucga     2100

uuagcuugau gugucucgca uuagggggag uucugauauu ccuguccacg gccgucucag     2160

cugacguggg cugcagugug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6181
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 2 opt

<400>  6181
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg auccugagaa      540

aucccggauu ugccuuggcu gccgcggcaa uagccuggcu ccugggcagc aguacauccc      600

agaaagugau cuaucugguc augauucucu uaaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uucguagaag ggauguccgg agguacuugg guugacgugg      720

uccuggagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcuuguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uacuuggaca      900

aacagagcga uacccaguau gugugcaaga gaacucuggu cgacaggggc ugggguaacg      960

gguguggacu auuuggcaaa ggcagucucg ugacaugcgc aaaguucgcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucuggaaua ccggauuaug cuuagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucug ggaggguuug     1200

gcuccuuggg ucucgauugu gaaccuagaa ccgggcugga cuucagugau uuauauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuucacgac auuccacuuc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucugguag     1380

aguucaaaga cgcgcacgca aagaggcaga ccguuguggu cuugggcagc caggaggggg     1440

ccgugcauac cgcucuagcc ggagcacugg aagccgagau ggauggugcu aagggccggc     1500

ucuccucugg gcaccugaaa ugccgccuua agauggacaa acugagauug aagggaguca     1560

gcuauucacu cuguacugcc gcauuuacgu ucacaaaaau accugcggaa acccugcaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag accuuaacuc cugugggcag gcugaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcuaga gcucgauccc ccguuuggag     1800

acagcuacau cgugauaggc auuggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uucgaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ugggggacac agccugggau uuuggaagug uaggcggugc gcuuaacagc uuggggaaag     1980

gcauucacca gaucuucgga gccgcuuuca agucccuguu uggcgggaug ucuugguucu     2040

cgcaaauucu cauaggaacu cugcuuaugu ggcugggcuu gaauaccaag aacgguagca     2100

ucucccucau gugucuggca uuagggggag ugcugauuuu ucuaucaacg gccguuagug     2160

cugacgucgg cugcucugug gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6182
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 2 opt

<400>  6182
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuuc aaccacggug aguaauaugg cugaaguacg uagcuacugc uaugaggcca      840

cuauuucuga uaucgcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccgccguugu gcugggcagc caggaggggg     1440

cuguccauac cgccuuggca ggagcccuag aagcugagau ggauggugcc aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugca gcguucacgu uuacaaaaau cccugcugaa acccuccaug     1620

gcacugucac cguggagguu caguacgccg guacagacgg gccaugcaag gugcccgcac     1680

aaauggccgu agauaugcag acccugacuc cugucggcag guuaauuaca gcuaacccag     1740

ugaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaagcc uuugaggcaa ccguccgggg cgcuaagcga auggccgugc     1920

ucggggacac agcgugggau uucggaagug uaggcggugc ccugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gcugcauuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgcc uuagggggag ugcugauuuu ccugucaacg gcuguuagug     2160

ccgacgucgg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6183
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 2 opt

<400>  6183
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacagcc cccgcgcuga ggccacucuu gggggauucg     1200

gcucacuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcacc caggaggggg     1440

ccgugcauac ugcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cuuauagcuu guguacggcc gcauucacau uuaccaaaau accugcggaa acucuccaug     1620

gcaccgugac aguugaggug caguacgcug guaccgacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acucugacac cugugggcag guuaaucacg gccaacccag     1740

uuauuaccga aucaacugag aauaguaaga ugaugcugga gcuagauccc ccguucggag     1800

acuccuacau cgugauaggc gucggggaga aaaagauuac acaccacugg caucguagcg     1860

gaucuaccau cgguaaggcu uuugaagcca cagugcgggg cgcaaaacga auggcuguac     1920

ucggggacac ugccugggau uucggaucag ugggcggugc gcugaacagu cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agagcuuguu cggcgggaug uccugguuuu     2040

cucaaauccu gauuggaacc cuccugaugu ggcuuggccu gaauacgaag aacgguucga     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccuguccacc gccgucucag     2160

cugacguggg cugcagugug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6184
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 2 opt

<400>  6184
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugagcug caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag accuuaacuc cugucggcag gcugaucaca gccaacccag     1740

ugauuacgga aaguaccgaa aauuccaaga ugaugcuaga gcucgauccc ccguucggag     1800

acagcuacau cguuauaggc gugggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccguccgggg cgcaaaacga auggcugugc     1920

ugggggacac agccugggau uucggaagug uaggcggugc gcuuaacagc uuggggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu cauuggaacu cugcuuaugu ggcugggcuu gaauaccaag aacgguagca     2100

uaucccucau gugucuggca uuagggggag ugcugaucuu ccuaucaacg gccguuagug     2160

cugacgucgg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6185
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 2 opt

<400>  6185
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga aaccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg agguuacgcg acgugguucc gcuuacuaua      180

uguaccugga cagaaaugau gccggcgaag caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggacuuag gacacaugug ugaugccacu augucauacg      300

agugcccuau gcuggacgaa ggcguggagc cagaugacgu agauuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaauc acgcgaguac accaagcacc uuauucgaau cgaaaacugg auauuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa ucgccugguu gcucggcagc aguacauccc      600

agaaaguuau uuaucuggug augauccugc ucauugcucc ugccuacucu aucaggugca      660

uaggcgucag caaccgggac uuuguggagg ggauguccgg agguacuugg guagauguug      720

uguuagagca uggcgggugu gucaccguga uggcacagga caagccaaca guggauauug      780

aacuggucac cacgacuguu ucaaauaugg cugaggugcg uaguuacugc uaugaagcca      840

gcaucucuga cauggcgucc gauucacgcu gucccacaca aggagaggcc uaccuugaca      900

aacagagcga cacccaguau guaugcaaga gaacuuuggu ggauaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaaa aucucgagua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgacacggg ccaugagacc gaugaaaauc     1140

gagccaaagu ggagauuaca ccaaacucac cccgcgcuga agccacucuu gggggauucg     1200

gcucccuggg uuuggacugu gagccuagaa ccgggcucga uuuuagugac cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagaaug guuccacgau auaccacucc     1320

cguggcacgc aggcgccgac acuggaacac cccauuggaa uaacaaggag gcucuugugg     1380

aguuuaaaga ugcgcacgca aagaggcaga ccguaguugu gcugggcagc caggaagggg     1440

ccguccauac cgcuuuggcc ggagcacuag aggccgaaau ggacggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggauaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggag acccuccaug     1620

gcacugucac cguggaaguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agacaugcag acccugacuc cugucggcag guuaauuaca gccaacccag     1740

ugaucacgga gaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau aguuauuggc gugggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaagcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggauac agccugggac uucggaagug uaggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag ugcugauuuu ccugucaacg gccguuagug     2160

cugaugucgg cugcucugug gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6186
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 2 opt

<400>  6186
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga aaguaucgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau agugauuggc gucggggaga aaaagaucac ccaccacugg caucguucug     1860

gaucaacuau cgguaaggcu uuugaggcca cagugcgggg cgcaaaacga auggcuguac     1920

ucggggacac cgccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaaca cuccugaugu ggcuuggccu gaauacuaag aacgguagca     2100

ucuccuugau gugucucgca uuagggggag uucugauuuu ccugucaacc gccgucagug     2160

cugacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6187
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 2 opt

<400>  6187
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggaugguguc aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcu gccuucacgu uuacaaaaau accugcagaa acccuccaug     1620

gcacuguuac cguggaggua caguacgcgg guacagacgg gccaugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacuc cuguuggcag guuaaucaca gcaaacccag     1740

ugauuacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgucauaggc gugggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaagcc uuugaggcua ccguacgggg cgccaagcga auggcagugc     1920

ucggggacac agcuugggau uucggaagug uuggcggugc ccugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gcggccuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuucau guguuuggcu cucgggggag uccugaucuu uuuaucaacg gcagugagug     2160

ccgacguggg cugcucuguc gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6188
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 2 opt

<400>  6188
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggcaaac     1500

uguccucugg gcaccucaag ugccggcuga aaauggacaa gcugcgccug aaaggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaagau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag auuaaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaaa ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga agaaaauuac ucaccacugg cauaggucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgugg cgcaaaacgg auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggaagg     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaaa aacgguagca     2100

uauccuugau gugucucgca cuugggggag uuuuaaucuu ccugucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6189
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 2 opt

<400>  6189
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga ggcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaagggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aggccgaaau ggacggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggauaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggag acccuccaug     1620

gcacugugac cguugaagug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgacaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga gaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaagcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggauac agccugggac uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacg gccgucagug     2160

cugauguggg cugcucugug gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6190
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 2 opt

<400>  6190
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug gugccgacgg gccaugcaag guacccgcac     1680

aaauggccgu cgauaugcag acacugaccc cugugggcag guuaaucacu gcuaacccag     1740

uuauuacaga aaguacggaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ccaccacugg caucguucug     1860

gaucaacuau cgguaaagcc uuugaggcaa cagugcgggg cgcuaagcga auggccguac     1920

ucggggacac cgcgugggau uucggaagug ugggcggugc ccugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gcugcauuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaaca cuccugaugu ggcuuggccu gaauacuaag aacgguagca     2100

uauccuugau gugucucgcc uuagggggag uucugaucuu ccugucaacc gcugucagug     2160

ccgacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6191
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 2 opt

<400>  6191
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc cggcaaggag aaaaagaggc gcggggcuga caccaaugug ggaauagucg      120

gucugcuccu uacaacugca auggccgcgg aaguuacgcg gcgaggcagc gcuuacuaua      180

uguaccugga ucguaacgac gccggggagg caaucuccuu ccccaccaca uugggaauga      240

auaaaugcua uauucagauc auggauuuag gccacaugug ugacgccacu augucuuacg      300

aaugcccuau gcuggaugag gguguggaac cagacgaugu agacuguugg ugcaacacca      360

caucaaccug gguggucuau gggacuuguc aucacaagaa aggagaggcu agaaggaguc      420

ggcgcgcagu gacacuaccg agccauucca ccagaaagcu ccaaacgagg ucucagacuu      480

ggcuggaguc gcgggaauac accaagcacc uuauucgcgu ugagaauugg aucuuucgaa      540

accccggcuu cgcccuggcu gccgcggcaa uagccugguu gcucggcuca agcacaaguc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucc auuagaugca      660

ucgggguguc uaauagggau uuuguagaag gaaugagcgg uggcacuugg guugacgugg      720

ucuuagagca ugggggaugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug uccaacaugg cugaaguacg gucauacugc uaugaggcca      840

guauuagcga uauggcgucu gacucccguu gucccacaca aggcgaagcc uaccuugaca      900

aacagucaga uacccaguau gugugcaagc gcacuuuggu cgacagaggu ugggggaaug      960

gauguggccu guucggcaaa gggagccuag ugacaugcgc aaaguuugcu ugcaguaaga     1020

aaaugaccgg aaagucuauc cagccugaga accucgaaua caggauuaug cuguccguuc     1080

acgguagcca acacucgggc augaucguga augauacggg gcaugagacc gacgagaacc     1140

gggccaaagu cgaaaucaca ccaaauucuc cccgagcuga ggccacucuu ggaggcuucg     1200

guucccuggg guuggauugu gaaccucgca ccggccucga cuuuucagau cuguauuacu     1260

uaacaaugaa caauaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggagccgau acuggcacac cccauuggaa caacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagagacaga ccguuguggu ccuggggagu caggagggag     1440

ccgugcauac cgcuuuggcc ggugcacuag aagccgagau ggauggcgcu aaggggaggc     1500

ugagcuccgg acaccucaaa ugccggcuga agauggacaa acuucgccug aagggcgucu     1560

cuuauagcuu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

guacugugac cguugaggug caguacgcug ggacagacgg cccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggaag auuaaucaca gccaauccag     1740

uuauuacgga aucaaccgag aacaguaaga ugaugcugga gcuagauccc ccguucggcg     1800

acuccuacau cgugauaggg gucggagaga aaaagauuac ucaccacugg cauaggagcg     1860

guucuacaau cggcaaggcu uuugaagcca ccgugcgugg ggcacggcga auggcuguac     1920

ucggagacac agccugggau uucggcucag uggguggggc gcugaauagu cuuggcaaag     1980

gaauccacca gauuuucggc gccgcuuuua agagcuuguu cgggggaaug uccugguuuu     2040

cucaaauccu gauuggcacu cuccugaugu ggcuuggucu gaacaccaaa aaugggucga     2100

ucagcuugau gugucucgca cugggaggcg uuuuaauauu ccuguccacg gccgucucag     2160

cugacguggg uugcagugug gauuuuucua agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6192
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 2 opt

<400>  6192
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga aaccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg agguuacgcg acgugguucc gcuuacuaua      180

uguaccugga cagaaaagau gccggcgaag caaucucuuu ccccaccaca uuggggauga      240

auaagugcua uauucagauc auggacuuag gacacaugug ugaugccacu augucauacg      300

agugcccuau gcuggacgaa ggcguggagc cagaugacgu agauuguugg ugcaacacca      360

caaguaccug gguggucuau gguacuuguc aucacaaaaa gggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaauc acgcgaguac accaaacacc uuauucgagu ugaaaauugg aucuuuagaa      540

accccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaggugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaucgggac uuuguagagg ggauguccgg agguacuugg guugaugugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga caaaccaaca gucgauauag      780

aacugguuac cacgacugug ucaaacaugg cugagguacg uaguuacugc uaugaagcca      840

gcauuucuga cauggcgucc gauucacgcu gucccacaca aggagaggcc uaccuugaca      900

agcagagcga cacccaguau gugugcaaaa gaacuuuggu cgauaggggc ugggguaaug      960

gguguggacu guucggcaag ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaaa     1020

agaugaccgg gaaauccauc cagccugaaa accucgagua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga augacacggg ccaugagacc gaugaaaacc     1140

gagccaaggu cgagaucaca ccaaauucac cccgcgcuga agccacucuu gggggauucg     1200

gcucccuggg uuuggacugu gagccuagaa ccgggcucga uuuuagugac cuguauuacu     1260

uaacaaugaa caauaaacac uggcuggugc auaaggaaug guuccacgau auuccacucc     1320

cguggcacgc aggcgccgac acuggaacac cccauuggaa caauaaagag gcucuuguag     1380

aguuuaagga ugcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaagggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aggccgaaau ggacggugcu aaaggccggc     1500

uguccucugg gcaccucaag ugccgccuga aaauggauaa gcugagacug aaaggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaagau accugcggag acccuccaug     1620

gcacugugac cguugaagug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgacaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga gaguaccgag aacuccaaaa ugaugcugga acuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga agaaaauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaagcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggauac agccugggac uucggaagug ugggcggugc gcugaauagc cuugggaagg     1980

gcauccacca gauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaacaccaaa aaugguagca     2100

uauccuugau gugucucgca cuugggggag uuuuaaucuu ccugucaacg gccgucagug     2160

cugauguggg cugcucugug gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6193
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 2 opt

<400>  6193
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggugucc gacucacgcu gucccacaca aggagaagcg uaccuugaca      900

aacagagcga uacccaguau gucugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag uuacaugcgc caaguuugca ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcgugc     1080

acggaucgca acacucuggu augaucguca acgauacggg ccaugagacc gacgagaauc     1140

gagcuaaagu ggaaaucaca ccaaacucac cccgcgccga ggcuacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcugguac auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcagau acuggaacac cccauuggaa uaacaaggaa gcccuuguug     1380

aguuuaaaga cgcucacgcg aagaggcaga ccguggucgu gcugggcagc caggaggggg     1440

caguccauac cgccuuggcu ggagcccuag aagcagagau ggauggugcc aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguga     1560

gcuauucauu guguacugcu gccuucacgu uuacaaaaau accugcagaa acccuccaug     1620

gcacuguuac cguggaggua caguacgcgg guacagacgg gccaugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacuc cuguuggcag guuaaucaca gcaaacccag     1740

ugauuacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgucauaggc gugggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaagcc uuugaggcua ccguacgggg cgccaagcga auggcagugc     1920

ucggggacac agcuugggau uucggaagug uuggcggugc ccugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gcggccuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgcu uuagggggag uccugaucuu ccugucaacg gcagugagug     2160

ccgacguggg cugcucuguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6194
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 2 opt

<400>  6194
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccaaugug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguagc gcuuacuaua      180

uguaccugga uagaaacgac gccggcgagg caaucuccuu ccccaccaca uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucuuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaacauga      360

ccucaacaug gguggucuau gguaccuguc aucacaagaa aggggaggcu aggcggaguc      420

gcagagcagu gacucuaccg agccauucca caaggaagcu ccaaacccgg ucucagacgu      480

ggcuggaguc gcgcgaauac acuaagcacc uuauucgagu ugagaauugg aucuuuagaa      540

accccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcuca agcaccaguc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucc auuaggugca      660

ucggcguguc uaaucgggau uuuguagaag ggaugagcgg agguacaugg guugacgugg      720

ucuuagagca uggcgggugu gugacuguga uggcacagga uaagccaacc gucgacauag      780

agcugguuac aaccacggug uccaacaugg cugaaguacg uucauacugc uaugaggcca      840

guauuagcga uauggcgucu gacucccgcu gucccacuca aggagaagcc uaccuugaca      900

aacagucaga uacacaguau gugugcaaga gaaccuuggu cgacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcagccuag ugacuugcgc aaaguuugcu ugcaguaaga     1020

aaaugacagg gaagucuauc cagccugaga accucgaaua ccggauuaug cuguccguuc     1080

acggaagcca acacucgggu augaucguga augauaccgg ccaugagacg gacgagaacc     1140

gagccaaagu cgaaaucacc ccaaauucuc cccgcgcuga ggccacacuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa cugggcucga cuuuucagau cuguauuacu     1260

uaaccaugaa caauaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acaggaacuc cccauuggaa caacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagu caggaggggg     1440

ccgugcauac agcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cuuauagcuu guguaccgcc gcauucacuu uuacgaaaau accugcggaa acacuccaug     1620

gcaccgugac uguugaggug caguacgcug guaccgacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acacugaccc cugugggcag guuaaucacu gccaauccag     1740

uuauuacaga aucaacggag aacaguaaga ugaugcugga gcuagauccc ccguucggag     1800

acuccuacau cgugauaggc gucggggaga aaaagauuac ccaccacugg caucguagcg     1860

gaucuacuau cgguaaggcu uuugaagcca cagugcgggg cgcaaaacga auggcuguac     1920

ucggggacac cgccugggau uucggaucag ugggcggugc gcugaauagu cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agagcuuguu cggcgggaug uccugguuuu     2040

cucaaauccu gauuggaaca cuccugaugu ggcuuggccu gaacacuaag aaugguucga     2100

ucagcuugau gugucucgca uuagggggag uucugauauu ccuguccacc gccgucucag     2160

cugacguggg cugcagugug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6195
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 2 opt

<400>  6195
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaaucaca ccaaacucac cccgcgcuga ggccgcacuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa cugggcucga cuuuagugau cuguauuacu     1260

uaaccaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcugau acaggaacuc cccauuggaa uaacaaggaa gcgcuuguag     1380

aguuuaaaga cgcacacgcc aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

cugugcauac agccuuggca ggagcccuag aagcugagau ggauggugcc aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguaccgca gcguucacuu uuacgaaaau accugcugaa acacuccaug     1620

gcaccgugac uguugaggug caguacgccg guaccgacgg gccaugcaag guacccgcac     1680

aaauggccgu cgauaugcag acacugaccc cugugggcag guuaaucacu gcuaacccag     1740

uuauuacaga aaguacggaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ccaccacugg caucguucug     1860

gaucaacuau cgguaaagcc uuugaggcaa cagugcgggg cgcuaagcga auggccguac     1920

ucggggacac cgcgugggau uucggaagug ugggcggugc ccugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gcugcauuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaacacu gaucggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uuuccuugau gugucucgcc uuagggggag uucugauauu ccugucaacg gcugucagug     2160

ccgacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6196
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 2 opt

<400>  6196
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga aaccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg agguuacgcg acgugguucc gcuuacuaua      180

uguaccugga cagaaaugau gccggcgaag caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggacuuag gacacaugug ugaugccacu augucauacg      300

agugcccuau gcuggacgaa ggcguggagc cagaugacgu agauuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaauc acgcgaguac accaagcacc uuauucgagu ugaaaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcuuu agccuacucu auuaggugca      660

ucggcgugag caaccgggac uuuguagagg ggauguccgg agguacuugg guugaugugg      720

uccuggagca uggcgggugu gugaccguga uggcacagga caagccuaca gucgauauag      780

aacuuguuac cacgacugug ucaaauaugg cugagguacg uaguuacugc uaugaagcca      840

gcauuucuga cauggcgucc gauucacgcu guccaacaca aggagaggcc uacuuggaca      900

aacagagcga cacccaguau gugugcaaga gaacucuggu cgauaggggc ugggguaacg      960

gguguggacu auucggcaaa ggcagucucg ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc cagcccgaaa aucuggagua ccggauuaug cuuagcguuc     1080

acggaucgca acacucuggu augaucguga acgacacggg ccaugagacc gaugaaaauc     1140

gagccaaagu cgagaucaca ccuaacuccc cacgcgcuga agccacucug ggaggguucg     1200

gcucauuggg ucucgacugu gagcccagaa ccgggcugga uuuuagugac uuauauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagaaug guuccacgau auuccucuuc     1320

cauggcacgc aggcgccgac acuggaaccc cgcauuggaa uaacaaggag gcucugguag     1380

aguuuaaaga ugcgcacgca aagaggcaga caguuguggu cuugggcagc caggaagggg     1440

ccgugcauac cgcucuagcc ggagcacugg aggccgaaau ggacggugcu aagggccggc     1500

ucuccucugg gcaccugaaa ugccgccuua agauggauaa acugagauug aagggaguca     1560

gcuauucacu cuguacugcc gcauucacgu uuacaaaaau acccgcggag acccugcaug     1620

gcacugugac cguugaagug caguacgcug guacagacgg gccuugcaag guaccagccc     1680

aaauggcagu cgacaugcag accuuaacuc ccgugggcag gcugaucaca gccaacccug     1740

uuauuacgga gaguaccgaa aauuccaaga ugaugcuaga gcucgaucca cccuucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaagcca ccgugcgggg cgcaaaacga auggcuguac     1920

ugggggauac agccugggac uucggaagug ugggcggugc gcuuaacagc uuggggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu cauuggaacu cugcuuaugu ggcugggcuu gaauaccaag aacgguagca     2100

uaucccucau gugucuggca uuagggggag uucugaucuu ccuaucaacg gccgucagug     2160

cugauguggg cugcucugug gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6197
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 2 opt

<400>  6197
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcauaag caaccgggau uuuguggaag ggauguccgg agguacuugg guagacguug      720

uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca guggacauug      780

agcuggucac cacgacuguu ucaaauaugg cugaagugcg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcgugc     1080

acggaucgca acacucuggu augaucguua acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu ggaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcauacuggg uuuggauugu gaaccuagaa ccgggcucga cuuuuccgau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac aucccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuugugg     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguaguugu gcugggcagu caggaggggg     1440

ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagugu     1560

cuuauagcuu guguacugcc gcauucacgu uuacaaaaau uccugcggaa acccuccaug     1620

gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag gugcccgccc     1680

aaauggcagu agauaugcag acccugacuc cugucggcag guuaaucaca gccaacccag     1740

ugauaacgga aucaaccgag aauaguaaga ugaugcugga gcuagauccc ccguucggag     1800

acuccuacau uguuaucggc gugggggaga aaaagaucac ucaccacugg caucguagcg     1860

gaucuacaau ugguaaggcu uuugaagcca ccguccgggg cgcaaaacga auggcugugc     1920

ucggggacac agccugggau uucggaucag uaggcggugc gcugaacagu cuugggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agagcuuguu cggcgggaug uccugguuuu     2040

cucaaauacu gaucggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguucga     2100

uuagcuugau gugucucgca uuagggggag ugcugaucuu ccuguccacg gccguuucag     2160

cugacgucgg cugcagugug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6198
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 2 opt

<400>  6198
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacacuug ugacgccacc augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacaa      360

ccaguacuug gguggucuau gguacauguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacccuaccg ucccauucua uuaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauccgagu ugagaacugg auauuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa ucgccugguu gcucggcagc aguacauccc      600

agaaagugau uuaucugguc augauccugc ucauugcucc ugccuacucu aucaggugca      660

uaggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauug      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg uaguuacugc uaugaggcca      840

gcaucucuga uauggcgucc gacucacgcu gucccacaca aggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaga aucucgaaua ccggaucaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gagccaaagu cgaaauuaca ccaaacucac cccgcgcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auaccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau cccugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaauuaca gccaacccag     1740

uuaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau agugauuggc gucggggaga aaaagaucac ucaccacugg caucguucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgggg cgcaaaacga auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauucacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauucu gauaggaacu cuccugaugu ggcuuggccu gaaugcaaag aacgguagca     2100

ucuccuugau gugucucgcc uuagggggag uucugauuuu ccugucaacc gcugucagug     2160

ccgacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6199
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 2 opt

<400>  6199
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu caaaggagcc      420

ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccagacgagg ucgcagacuu      480

ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaacugg aucuuucgaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuagaugca      660

ucggcgugag caacagggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacaaga uaagccaaca gucgacauag      780

agcugguuac cacgacugug ucaaauaugg cugaaguacg gaguuacugc uaugaggcca      840

gcauuucuga uauggcgucc gacucacguu gucccacaca gggagaagcc uaccuugaca      900

aacagagcga uacccaguau gugugcaagc gcacuuuggu cgacagaggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacaugcgc aaaguuugcu ugcucuaaga     1020

aaaugaccgg gaaguccauc caaccugaga aucucgaaua caggauuaug cugagcguuc     1080

acggaucgca gcacucuggu augaucguga acgauacggg ccaugagacc gacgagaauc     1140

gggccaaagu cgaaaucaca ccaaacucac cccgagcuga ggccacucuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau cuguauuacu     1260

uaacaaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa uaacaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagagacaga ccguuguggu ccugggcagc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggcaggc     1500

uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug aagggaguca     1560

gcuauucauu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caauacgcug guacagacgg gccaugcaag guacccgccc     1680

agauggcagu cgauaugcag acccugacuc cugugggcag auuaaucaca gccaacccag     1740

uuauuacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg cauaggucug     1860

gaucaacaau cgguaaggcu uuugaggcca ccgugcgugg cgcaaaacgg auggcuguac     1920

ucggggacac agccugggau uucggaagug ugggcggugc gcugaacagc cuugggaaag     1980

gcauccacca aauuuucgga gccgcuuuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcagauccu gauuggaacu cuccugaugu ggcuuggccu gaauaccaag aacgguagca     2100

uauccuugau gugucucgca uuagggggag uucugaucuu ccugucaacg gccgucagug     2160

cugacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6200
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 2 opt

<400>  6200
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaagcccgg ucgcagacgu      480

ggcuggaguc acgcgaauac acuaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcucuggcc gcggcagcca uagcuugguu gcucggcagc aguaccuccc      600

agaaagugau cuaucugguc augauucugc ucaucgcccc ugcauacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacaugg guugacgugg      720

ucuuagagca uggcgggugu gugacuguga uggcucagga uaagccaacc gucgacauag      780

agcugguuac aaccacggug ucaaauaugg ccgaaguacg uaguuacugc uaugaggcga      840

gcauuucuga uauggccucc gacucacgcu gucccacuca aggagaagca uaccuugaca      900

aacagagcga uacacaguau gugugcaaga gaaccuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ugacuugcgc uaaguuugcc ugcucuaaga     1020

aaaugacagg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcguuc     1080

acggaucgca acacucuggu augaucguga acgauaccgg ccaugagacg gacgagaauc     1140

gagcuaaagu cgaaaucacc ccaaacucac cccgcgccga ggcaacacuu gggggauucg     1200

gcucccuggg uuuggauugu gaaccuagaa cugggcucga cuuuagugau cuguauuacu     1260

uaaccaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcugau acaggaacuc cccauuggaa uaacaaggaa gcgcuuguag     1380

aguuuaaaga cgcacacgcc aagaggcaga ccguuguggu ccugggcagc caggaggggg     1440

cugugcauac agccuuggca ggagcccuag aagcugagau ggauggugcc aagggccggc     1500

uguccucugg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguca     1560

gcuauucauu guguaccgca gcguucacuu uuacgaaaau accugcugaa acacuccaug     1620

gcaccgugac uguugaggug caguacgccg guaccgacgg gccaugcaag guacccgcac     1680

aaauggccgu cgauaugcag acacugaccc cugugggcag guuaaucacu gcuaacccag     1740

uuauuacaga aaguacggaa aauuccaaga ugaugcugga gcuagauccc ccguucggag     1800

acagcuacau cgugauaggc gucggggaga aaaagauuac ccaccacugg caucguucug     1860

gaucaacuau cgguaaagcc uuugaggcaa cagugcgggg cgcuaagcga auggccguac     1920

ucggggacac cgcgugggau uucggaagug ugggcggugc ccugaacagc cuugggaaag     1980

gcauccacca gauuuucgga gcugcauuua aguccuuguu cggcgggaug ucuugguuuu     2040

cgcaaauccu gauuggaaca cuccugaugu ggcuuggccu gaauacuaag aacgguagca     2100

uauccuugau gugucucgcc uuagggggag uucugaucuu ccugucaacc gcugucagug     2160

ccgacguggg cugcucugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6201
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 2 opt

<400>  6201
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaucugac gccggcgagg caaucucauu ccccaccaca uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augaguuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaacacca      360

caagcaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcgguccc      420

gcagagcagu gacacuaccg ucucauucga ccaggaagcu ccaaacgcgg ucacagacuu      480

ggcuggagag ccgcgaauac accaagcacc uuauucgagu ugagaauugg aucuuuagaa      540

accccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagu uccacaucuc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacagc auuaggugca      660

ucggcguguc caaucgggau uuuguagaag ggaugucagg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccguga uggcacagga uaagccaaca gucgacauag      780

agcugguuac cacgacugug aguaacaugg cugaaguacg uagcuacugc uaugaggccu      840

cuauuuccga uauggcguca gacagccgcu gucccacaca aggagaagcc uaccuugaca      900

aacagaguga uacccaguau gugugcaaga gaacuuuggu cgacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcucucuag ugacaugcgc aaaguuugcu ugcuccaaga     1020

aaaugaccgg gaaaagcauc cagccugaga accucgaaua ccggauuaug cugucgguuc     1080

acggaucuca acacuccggu augaucguga augauacggg ccaugagacc gacgagaacc     1140

gagccaaagu cgaaaucaca ccaaauucac cccgcgcuga ggccacucuu gggggauucg     1200

gcagucuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagcgau cuguauuacu     1260

uaacaaugaa caauaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa gcucuuguag     1380

aguuuaaaga cgcgcacgca aagaggcaga ccguuguggu ccugggcucc caggaggggg     1440

ccgugcauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu aagggccggc     1500

ugucuagcgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cauauaguuu guguacugcc gcauucacgu uuacaaaaau accugcggaa acccuccaug     1620

gcacugugac cguugaggug caguacgcug guacagacgg gccaugcaag guacccgccc     1680

aaauggcagu cgauaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

uuauuacgga auccaccgag aacagcaaga ugaugcugga gcuagauccc ccguucggag     1800

acucuuacau cgugauaggc gucggggaga aaaagauuac ucaccacugg caucguucag     1860

gaaguacaau cgguaaggcu uuugaagcca ccgugcgggg cgcaaagcga auggcuguac     1920

ucggggacac agccugggau uucggaagcg ugggcggugc gcuuaauucc cuggggaaag     1980

gcauccacca gauuuucgga gccgcuuuua agucuuuguu cggcgggaug ucgugguuua     2040

gccaaauccu gauuggaacu cuccugaugu ggcuuggccu gaacaccaag aaugguucca     2100

uaucauugau gugucucgca uuagggggag uucugaucuu ccugaguacg gccgucucug     2160

cugacguggg cugcagcgug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6202
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 2 opt

<400>  6202
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcaccga cacaagcgug gggauagucg      120

gacugcuccu uacuacggcu auggcagccg aaguuacccg acgugguucc gcguacuaua      180

uguaccugga uagaucugac gcuggcgagg ccaucucauu ccccacaacu uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgcaacc augaguuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaacacaa      360

ccagcacuug gguggucuau gguacauguc aucacaagaa aggggaggcc aggcgguccc      420

gcagagcugu gacccuaccg ucucauucga cgaggaagcu ccaaacucgg ucacagaccu      480

ggcuggagag ccgcgaauac acaaagcacc uuauucgagu ugagaauugg aucuuuagaa      540

accccggauu cgcacuggcc gcugccgcga uagcaugguu gcucggcagu uccacuucuc      600

agaaagugau cuaucugguc augauucugc ucaucgcccc ugcuuacagc auuaggugca      660

ucggcguguc caaucgggau uuuguagaag ggaugucagg agguaccugg guugacgugg      720

ucuuagagca uggcgggugu gugacaguga uggcccagga uaagccaacc gucgacauag      780

agcugguuac gacuacagug aguaacaugg cagaaguacg uagcuacugc uaugaggcuu      840

cuauuuccga uauggccuca gacagccgcu gucccaccca aggagaagcg uaccuugaca      900

aacagaguga uacucaguau gugugcaaga gaacauuggu cgacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcucucuag ugaccugcgc caaguuugca ugcuccaaga     1020

aaaugacggg gaaaagcauc cagccugaga accucgaaua ccggauuaug cugucgguuc     1080

acggaucuca acacuccggu augaucguga augauaccgg ccaugagaca gacgagaacc     1140

gagcuaaagu cgaaaucacu ccaaauucac cccgcgccga ggcuacccuu gggggauucg     1200

gcagucuggg uuuggauugu gaaccuagaa cagggcucga cuuuagcgau cuguauuacu     1260

uaacuaugaa caauaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacac cggcgccgau acaggaacuc cccauuggaa caauaaggaa gcacuuguag     1380

aguuuaaaga cgcccacgcu aagaggcaga ccguuguggu ccugggcucc caggaggggg     1440

cggugcauac ggcauuggcc ggagcucuag aagccgagau ggauggugca aagggccggc     1500

ugucuagcgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cauauaguuu guguacagcc gcuuucaccu uuacuaaaau accugccgaa acccuccaug     1620

gcacagugac cguugaggug caguacgcag guacugacgg gccaugcaag guacccgcgc     1680

aaauggcugu cgauaugcag acacugacgc cugugggcag guuaaucacc gccaacccag     1740

uuauuacuga aggaacagag aacuccaaga ugaugcugga gcuagauccc ccguucggcg     1800

acagcuacau cgugauaggg gucggagaaa agaagauuac ccaccacugg caucguucug     1860

guucaacaau cggcaaagca uuugaggcca cugugcgagg ggcuaagcgg auggccguac     1920

ucggagacac cgcaugggau uucggcagug uggguggggu ucugaauagc cuuggcaaag     1980

gaauccacca gauuuucggc gcugccuuua aguccuuguu cgggggaaug ucuugguuuu     2040

cgcaaauccu gauuggcacg cuccugaugu ggcuuggucu gaacaccaag aaugggagca     2100

uauccuugau gugucucgcg cugggaggcg ucuuaaucuu ccugucaaca gccgugagug     2160

cugacguggg uugcucuguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6203
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 2 opt

<400>  6203
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccgcgg aaguuacgcg acgugguucc gcuuacuaua      180

uguaccugga uagaaaugac gccggcgagg caaucucuuu ccccaccaca uuggggauga      240

acaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacu augucauacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaauacca      360

caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu aggcggagcc      420

gcagagcagu gacacuaccg ucccauucua ccaggaagcu ccaaacgcgg ucgcagacuu      480

ggcuggaguc acgcgaauac accaagcacc uuauucgagu ugagaacugg aucuuuagaa      540

aucccggauu cgcccuggcu gccgcggcaa uagccugguu gcucggcagc aguacauccc      600

agaaagugau cuaucugguc augauucugc ucaucgcucc ugccuacucu auuaggugca      660

ucggcgugag caaccgggau uuuguagaag ggauguccgg agguacuugg guugacgugg      720

ucuuagagca uggcgggugu gugaccgcaa uggcucagga uaagccaaca guggacauag      780

agcuggucac cacgacuguu ucaaauaugg ccgaagugcg uaguuacugc uaugaggcga      840

gcauuucuga uauggccucc gacucacgcu gucccacaca aggagaagca uaccuugaca      900

aacagagcga uacccaguau guaugcaaga gaacuuuggu ggacaggggc ugggguaacg      960

gguguggacu guucggcaaa ggcagucuag ucacaugcgc uaaguuugcc ugcucuaaga     1020

aaaugaccgg gaaguccauc cagccugaga aucucgaaua ccggauuaug cugagcgugc     1080

acggaucgca acacucuggu augcuuguua acgauacggg ccaugagacc gacgagaauc     1140

gagcuaaagu ggaaaucaca ccaaacucac cccgcgccga ggcaacucug ggaggguucg     1200

gcuccuuggg ucucgauugu gaaccuagaa ccgggcugga cuuuagugau uuauauuacc     1260

ugacaaugaa uaacaagcac uggcucgccc auaaagagug guuccacgac aucccacuuc     1320

cguggcacgc uggcgcggca acuggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga ugcucacgcc aagaggcaga ccgugguagu uuugggcagc caggaggggg     1440

cagugcauac cgcccuagcu ggagcccugg aagcagagau ggacggugcg aagggccggc     1500

ucuccucugg gcaccugaaa ugccgccuua agauggauaa acugagauug aagggaguca     1560

gcuauucacu cuguacugcu gccuucacgu uuacaaaaau uccugcagaa accguggacg     1620

gcacugucac cguggagggu caguacgggg gcacagacgg accaugcaag guucccgccc     1680

aaauggcugu ggauaugcag acccugacuc cuguaggcag guuaauaaca gccaacccag     1740

ucaucacgga aaguaccgaa aauuccaaga ugaugcugga gcuagauccc ccguucgggg     1800

acagcuacau ugugaucgga guuggugaga aaaagauaac ucaucacugg caccguucug     1860

gcucaacaau ugggaaagca uuugaggcua ccgugcgggg agccaagcga auggcggucc     1920

ucggcgacac agccugggau uucgguagug uagggggcgc ucugaacagc cuuggaaaag     1980

gcauccauca gaucauuggg gcagccuuca aguccuuguu uggaggcaug ucuugguucu     2040

cgcaaauccu gauugguacu cuccugaugu ggcuugggcu gaauaccaag aacggaagca     2100

uauccuugau gugucucgcu cugggcggug uguuaaucuu ucugucaacg gccgugagug     2160

gcgggguugg augcucuguc gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6204
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 2 opt

<400>  6204
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcaccga cacaagcgug gggauagucg      120

gacugcuccu uacuacggcu auggcagccg aaguuacccg acgugguucc gcguacuaua      180

uguaccugga uagaucugac gcuggcgagg ccaucucauu ccccacaacu uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgcaacc augaguuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaacacaa      360

ccagcacuug gguggucuau gguacauguc aucacaagaa aggggaggcc aggcgguccc      420

gcagagcugu gacccuaccg ucucauucga cgaggaagcu ccaaacucgg ucacagaccu      480

ggcuggagag ccgcgaauac acaaagcacc uuauucgagu ugagaauugg aucuuuagaa      540

accccggauu cgcacuggcc gcugccgcga uagcaugguu gcucggcagu uccacuucuc      600

agaaagugau cuaucugguc augauucugc ucaucgcccc ugcuuacagc auuaggugca      660

ucggcguguc caaucgggau uuuguagaag ggaugucagg agguaccugg guugacgugg      720

ucuuagagca uggcgggugu gugacaguga uggcccagga uaagccaacc gucgacauag      780

agcugguuac gacuacagug aguaacaugg cagaaguacg uagcuacugc uaugaggcuu      840

cuauuuccga uauggccuca gacagccgcu gucccaccca aggagaagcg uaccuugaca      900

aacagaguga uacucaguau gugugcaaga gaacauuggu cgacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcucucuag ugaccugcgc caaguuugca ugcuccaaga     1020

aaaugacggg gaagucuauc cagccugaga accucgaaua ccggauuaug cugucgguuc     1080

acggaucuca acacuccggu augaucguga augauaccgg ccaugagaca gacgagaacc     1140

gagcuaaagu cgaaaucacu ccaaauucac cccgcgccga ggcuacccuu gggggauucg     1200

gcagucuggg uuuggauugu gaaccuagaa cagggcucga cuuuagcgau cuguauuacu     1260

uaacuaugaa caauaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcagau accggaacac cccauuggaa caauaaggaa gcccuuguag     1380

aguuuaaaga cgcucacgcg aagaggcaga ccguuguggu ccugggcucc caggaggggg     1440

cagugcauac ugccuuggcu ggagcccuag aagcagagau ggauggugcc aagggccggc     1500

ugucuagcgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cauauaguuu guguacggcu gccuucacau uuuccaaaau accugcagaa acccuccaug     1620

gcacugugac cgaggaaguu caguacgcgg guacagacgg gccaugcaag gugcccgcuc     1680

aaauggccgu agauaugcag acccugacuc cugucggcag guuaaucaca gcaaacccag     1740

ugauuacgga gagcaccgag aacucuaaga ugaugcugga acuagauccc ccguucggag     1800

acucauacau cguuauaggc gugggggaga aaaagauuac ucaccacugg caucguagug     1860

gaagcacaau cgguaaagcc uuugaagcua ccguccgggg cgccaagcga auggcagugc     1920

ucggggacac agcuugggau uucggauccg uaggcggugc ccugaauagc cuugggaaag     1980

gcauccacca gauuuucgga gcggccuuua agucguuguu cggcgggaug agcugguuuu     2040

cccaaauccu gauuggaacu cuccugaugu ggcuuggccu gaacaccaag aaugguucaa     2100

uaaguuugau gugucucgcu uuagggggag ugcugaucuu ccugucuacg gcaguuagcg     2160

ccgacgucgg cugcuccgug gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6205
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 2 opt

<400>  6205
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcaccga cacaagcgug gggauagucg      120

gacugcuccu uacuacggcu auggcagccg aaguuacccg acgugguucc gcguacuaua      180

uguaccugga uagaucugac gcuggcgagg ccaucucauu ccccacaacu uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgcaacc augaguuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaacacaa      360

ccagcacuug gguggucuau gguacauguc aucacaagaa aggggaggcc aggcgguccc      420

gcagagcugu gacccuaccg ucucauucga cgaggaagcu ccaaacucgg ucacagaccu      480

ggcuggagag ccgcgaauac acaaagcacc uuauucgagu ugagaauugg aucuuuagaa      540

accccggauu cgcacuggcc gcugccgcga uagcaugguu gcucggcagu uccacuucuc      600

agaaagugau cuaucugguc augauucugc ucaucgcccc ugcuuacagc auuaggugca      660

ucggcguguc caaucgggau uuuguagaag ggaugucagg agguaccugg guugacgugg      720

ucuuagagca uggcgggugu gugacaguga uggcccagga uaagccaacc gucgacauag      780

agcugguuac gacuacagug aguaacaugg cagaaguacg uagcuacugc uaugaggcuu      840

cuauuuccga uauggccuca gacagccgcu gucccaccca aggagaagcg uaccuugaca      900

aacagaguga uacucaguau gugugcaaga gaacauuggu cgacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcucucuag ugaccugcgc caaguuugca ugcuccaaga     1020

aaaugacggg gaaaagcauc cagccugaga accucgaaua ccggauuaug cugucgguuc     1080

acggaucuca acacuccggu augaucguga augauaccgg ccaugagaca gacgagaacc     1140

gagcuaaagu cgaaaucacu ccaaauucac cccgcgccga ggcuacccuu gggggauucg     1200

gcagucuggg uuuggauugu gaaccuagaa cagggcucga cuuuagcgau cuguauuacu     1260

uaacuaugaa caauaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcagau accggaacac cccauuggaa caauaaggaa gcccuuguag     1380

aguuuaaaga cgcucacgcg aagaggcaga ccguuguggu ccugggcucc caggaggggg     1440

cagugcauac ugccuuggcu ggagcccuag aagcagagau ggauggugcc aagggccggc     1500

ugucuagcgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cauauaguuu guguacggcu gccuucacau uuaccaaaau accugcagaa acucuccaug     1620

gcaccgugac aguugaggug caguacgcgg guaccgacgg gccaugcaag guacccgcuc     1680

aaauggccgu cgauaugcag acucugacac cugugggcag guuaaucacg gcaaacccag     1740

uuauuaccga auccacugag aacagcaaga ugaugcugga gcuagauccc ccguucggag     1800

acucuuacau cgugauaggc gucggggaaa agaagauuac acaccacugg caucguucag     1860

gaaguaccau cgguaaagcc uuugaggcua cagugcgggg cgccaagcga auggcaguac     1920

ucggggacac ugcuugggau uucggaagcg ugggcggugc ccuuaauucc cuggggaaag     1980

gcauccacca gauuuucgga gcggccuuua agucuuuguu cggcgggaug ucgugguuua     2040

gccaaauccu gauuggaacc cuccugaugu ggcuuggccu gaacacgaag aaugguucca     2100

uaucauugau gugucucgcu uuagggggag uucugaucuu ccugaguacc gcagucucug     2160

ccgacguggg cugcagcgug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6206
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 2 opt

<400>  6206
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcaccga cacaagcgug gggauagucg      120

gacugcuccu uacuacggcu auggcaguug aagugacccg acgugguaau gccuacuaua      180

uguaccugga uagauccgac gcgggcgagg cuaucucuuu ccccacaacu auggggauga      240

acaaaugcua uauucagauc auggauuugg gacacaugug ugacgccacc augucauacg      300

aaugcccuau guuagaugag ggcguagaac cagacgaugu ggacuguugg ugcaauacaa      360

ccaguacuug ggucguguau gguacauguc aucacaagaa aggggaggca aggcggagcc      420

gcagagccgu uacccugccg ucccauucua cgaggaagcu acaaacucgg ucgcagaccu      480

ggcucgaguc acgcgaauac acaaagcacc ugauucgagu ggagaacugg aucuuuagaa      540

aucccggauu cgcucuugca gccgcugcca uagcguggcu guugggcagc aguacuuccc      600

agaaagucau cuaucucgug augauucugc ugaucgcacc cgccuacucu auuaggugca      660

ucggcguaag caaccgggau uuuguugaag ggauguccgg agguaccugg guggacgucg      720

ugcucgagca uggcgggugu gugacaguca uggcucagga uaagccaacc guugacauag      780

aguuagugac gacuacagua ucaaauaugg ccgaagugcg uaguuacugc uaugaggcaa      840

gcauuucuga uauggcuucc gacucacgcu gucccaccca aggagaagcc uaccuggaca      900

aacagagcga uacucaguau gucugcaaga gaacacuugu ggacaggggc ugggguaacg      960

gguguggauu guucggcaaa ggcagucugg uuaccugcgc gaaguuugcc ugcucuaaga     1020

aaaugacggg gaaguccauc cagccugaga aucuagaaua ccggauuaug cucagcgugc     1080

acggaucgca acacucuggu augaucguca acgauaccgg ccaugagaca gacgagaauc     1140

gagcaaaagu ggaaaucacu ccaaacuccc cucgcgcuga ggccacccuu gggggauucg     1200

gcucacuggg ucuggauugu gaaccuagaa caggguugga cuuuagugau cucuauuacc     1260

ugacuaugaa uaacaagcac ugguuaguac auaaagagug guuccacgac auuccacugc     1320

cguggcacgc uggcgccgau accggaacac cccauuggaa uaacaaggaa gcacucguug     1380

aguuuaaaga cgcccacgcu aagaggcaga ccguggucgu gcuuggcagc caggaggggg     1440

cgguccauac ugcacuggcc ggagcuuugg aagccgagau ggauggugca aagggccggc     1500

uauccucugg gcaccugaaa ugccgccuca agauggacaa acugagacuu aagggaguga     1560

gcuauucacu guguacggcc gcuuucacau uuaccaaaau accugccgaa acuuugcaug     1620

gcaccguuac aguggaggua caguacgcag guaccgacgg gccaugcaag guccccgcgc     1680

aaauggcugu ggauaugcag acucucacac cuguuggcag gcugaucacg gccaacccag     1740

ugauuaccga aaguacugaa aauuccaaga ugauguuaga gcuggauccc ccguucggag     1800

acagcuacau cgucauaggc gugggggaga aaaagauuac acaccacugg caucguucug     1860

gaucaaccau cgguaaagca uuugaggcca caguacgggg cgcuaagcga auggccgugc     1920

uaggggacac ugcaugggau uucggaagug uuggcggugc ucucaacagc cuggggaaag     1980

gcauccacca gauuuucgga gccgcguuua agucccuuuu cggcgggaug ucuugguuuu     2040

cgcaaaucuu gauuggaacc cugcucgucu ggcugggccu uaauacgaag aacgguagca     2100

uaucccugau guguuuggcc cucgggggag ugcugaucuu cuuaucaacc gcugugagug     2160

cagacgucgg cugcucuguu gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6207
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 2 opt

<400>  6207
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcaccga cacaagcgug gggauagucg      120

gacugcuccu uacuacggcu auggcagccg aaguuacccg acgugguucc gcguacuaua      180

uguaccugga uagaucugac gcuggcgagg ccaucucauu ccccacaacu uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgcaacc augaguuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg ugcaacacaa      360

ccagcacuug gguggucuau gguacauguc aucacaagaa aggggaggcc aggcgguccc      420

gcagagcugu gacccuaccg ucucauucga cgaggaagcu ccaaacucgg ucacagaccu      480

ggcuggagag ccgcgaauac acaaagcacc uuauucgagu ugagaauugg aucuuuagaa      540

accccggauu cgcacuggcc gcugccgcga uagcaugguu gcucggcagu uccacuucuc      600

agaaagugau cuaucugguc augauucugc ucaucgcccc ugcuuacagc auuaggugca      660

ucggcguguc caaucgggau uuuguagaag ggaugucagg agguaccugg guugacgugg      720

ucuuagagca uggcgggugu gugacaguga uggcccagga uaagccaacc gucgacauag      780

agcugguuac gacuacagug aguaacaugg cagaaguacg uagcuacugc uaugaggcuu      840

cuauuuccga uauggccuca gacagccgcu gucccaccca aggagaagcg uaccuugaca      900

aacagaguga uacucaguau gugugcaaga gaacauuggu cgacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcucucuag ugaccugcgc caaguuugca ugcuccaaga     1020

aaaugacggg gaaaagcauc cagccugaga accucgaaua ccggauuaug cugucgguuc     1080

acggaucuca acacuccggu augaucguga augauaccgg ccaugagaca gacgagaacc     1140

gagcuaaagu cgaaaucacu ccaaauucac cccgcgccga ggcuacccuu gggggauucg     1200

gcagucuggg uuuggauugu gaaccuagaa cagggcucga cuuuagcgau cuguauuacu     1260

uaacuaugaa caauaagcac uggcuggugc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcagau accggaacac cccauuggaa caauaaggaa gcccuuguag     1380

aguuuaaaga cgcucacgcg aagaggcaga ccguuguggu ccugggcucc caggaggggg     1440

cagugcauac ugccuuggcu ggagcccuag aagcagagau ggauggugcc aagggccggc     1500

ugucuagcgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagucu     1560

cauauaguuu guguacggcu gccuucacau uuaccaaaau accugcagaa acucuccaug     1620

gcaccgugac aguugaggug caguacgcgg guaccgacgg gccaugcaag guacccgcuc     1680

aaauggccgu cgauaugcag acucugacac cugugggcag guuaaucacg gcaaacccag     1740

uuauuaccga auccacugag aacagcaaga ugaugcugga gcuagauccc ccguucggag     1800

acucuuacau cgugauaggc gucggggaaa agaagauuac acaccacugg caucguucag     1860

gaaguaccau cgguaaagcc uuugaggcua cagugcgggg cgccaagcga auggcaguac     1920

ucggggacac ugcuugggau uucggaagcg ugggcggugc ccuuaauucc cuggggaaag     1980

gcauccacca gauuuucgga gcggccuuua agucuuuguu cggcgggaug ucgugguuua     2040

gccaaauccu gauuggaacc cuccugaugu ggcuuggccu gaacacgaag aaugguucca     2100

uaucauuggu uugucucgcu uuagggggag uccugaucuu ccugaguacc gcagugucug     2160

ccgacguggg cugcagcguc gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6208
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 2 opt

<400>  6208
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcaccga cacaagcgug gggauagucg      120

gacugcuccu uacuacggcu auggcaguug aagugacccg acgugguucc gccuacuaua      180

uguaccugga uagaucugac gcgggcgagg cuaucucauu ccccacaacu uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgccacc augaguuacg      300

aaugcccuau gcuggaugag ggcguagaac cagacgaugu ggacuguugg ugcaacacaa      360

ccagcacuug ggucguguau gguacauguc aucacaagaa aggggaggca aggcgguccc      420

gcagagccgu uacccuaccg ucucauucga cgaggaagcu ccaaacucgg ucacagaccu      480

ggcuggagag ccgcgaauac acaaagcacc uuauucgagu ggagaauugg aucuuuagaa      540

accccggauu cgcucuggca gccgcugcca uagcgugguu gcucggcagu uccacuucuc      600

agaaagucau cuaucuggug augauucugc ucaucgcccc ugcauacagc auuaggugca      660

ucggcguauc caaucgggau uuuguugaag ggaugucagg agguaccugg guggacgucg      720

uguuagagca uggcgggugu gugacaguca uggcucagga uaagccagcc guugacauag      780

agcuggugac cacgacugua aguaacaugg cagaagugcg uagcuacugc uaugaggcuu      840

cuauuuccga uauggccuca gacagccgcu gucccacaca aggagaagcg uaccuugaca      900

aacagaguga uacccaguau gucugcaaga gaacuuuggu ggacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcucucuag uuacaugcgc caaguuugca ugcuccaaga     1020

aaaugaccgg gaaaagcauc cagccugaga accucgaaua ccggauuaug cugucggugc     1080

acggaucuca acacuccggu augaucguca augauacggg ccaugagacc gacgagaacc     1140

gagcuaaagu ggaaaucaca ccaaauucac cccgcgccga ggcuacucuu gggggauucg     1200

gcagucuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagcgau cuguauuacu     1260

uaacaaugaa caauaagcac uggcugguac auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcagau acuggaacac cccauuggaa caauaaggaa gcccuuguug     1380

aguuuaaaga cgcucacgcg aagaggcaga ccguggucgu gcugggcucc caggaggggg     1440

caguccauac cgccuuggcu ggagcccuag aagcagagau ggauggugcc aagggccggc     1500

ugucuagcgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggagugu     1560

cauauaguuu guguacugcu gccuucacgu uuacaaaaau accugcagaa acccuccaug     1620

gcacuguuac cguggaggua caguacgcgg guacagacgg gccaugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacuc cuguuggcag guuaaucaca gcaaacccag     1740

ugauuacgga auccaccgag aacagcaaga ugaugcugga gcuagauccc ccguucggag     1800

acucuuacau cgucauaggc gugggggaaa agaagauuac ucaccacugg caucguucag     1860

gaaguacaau cgguaaagcc uuugaggcua ccguacgggg cgccaagcga auggcagugc     1920

ucggggacac agcuugggau uucggaagcg uuggcggugc ccuuaauucc cuggggaaag     1980

gcauccacca gauuuucgga gcggccuuua agucuuuguu cggcgggaug ucgugguuua     2040

gccaaauccu gauuggaacu cuccuggucu ggcuuggccu gaacaccaag aaugguucca     2100

uaucauugac gugucucgcu uuagggggag ugcugaucuu ccugaguacc gcagugucug     2160

ccgacgucgg cugcagcguu gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6209
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 2 opt

<400>  6209
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccguug aagugacgcg acgugguucc gcguacuaua      180

uguaccugga uagaucugac gcuggcgagg ccaucucauu ccccaccaca uuggggauga      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgcaacu augaguuacg      300

aaugcccuau gcuggaugag ggcguagaac cagacgaugu ggacuguugg ugcaacacca      360

caagcaccug ggucguguau gguacuuguc aucacaagaa aggggaggcc aggcgguccc      420

gcagagcugu uacacuaccg ucucauucga ccaggaagcu ccaaacgcgg ucacagacuu      480

ggcuggagag ccgcgaauac accaagcacc uuauucgagu ggagaauugg aucuuuagaa      540

accccggauu cgcacuggcc gcugccguca uagcgugguu gcucggcagu uccacaucuc      600

agaaagugau cuaucuggua augauucugc ucaucgcccc ugcauacagc auuaggugca      660

ucggcguuuc caaucgggau uuuguggaag ggaugucagg agguacuugg gucgacgugg      720

uguuagagca uggcgggugu gucaccguua uggcucagga uaagccaaca guggacauag      780

agcugguaac cacgacugug aguaacaugg ccgaaguccg uagcuacugc uaugaggcau      840

cuauuuccga uauggcuuca gacagccgcu gucccacaca aggagaagcc uaccuugaca      900

aacagaguga uacccaguau gugugcaaga gaacuuuggu ugacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcucucuag ugacaugcgc gaaguuugcc ugcuccaaga     1020

aaaugaccgg gaaaagcauc cagccugaga accucgaaua ccggauuaug cugucggucc     1080

acggaucuca acacuccggu augaucguga augauacggg ccaugagacc gacgagaacc     1140

gagcaaaagu agaaaucaca ccaaauucac cccgcgcuga ggccacucuu gggggauucg     1200

gcagucuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagcgau cuguauuacu     1260

uaacaaugaa caauaagcac uggcugguuc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc uggcgccgau acuggaacac cccauuggaa caauaaggaa gcacuugugg     1380

aguuuaaaga cgcccacgcu aagaggcaga ccgucguggu ccugggcucc caggaggggg     1440

cggugcauac cgcauuggcc ggagcucuag aagccgagau ggauggugca aagggccggc     1500

ugucuagcgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguuu     1560

cauauaguuu guguacugcc gcuuucacgu uuacaaaaau accugccgaa acccuccaug     1620

gcacugugac cguagagguc caguacgcag guacagacgg gccaugcaag gugcccgcgc     1680

aaauggcugu ugauaugcag acccugacuc cugugggcag guuaaucaca gccaacccag     1740

ucauuacgga auccaccgag aacagcaaga ugaugcugga gcuagauccc ccguucggag     1800

acucuuacau cgugauaggc guaggggaaa agaagauuac ucaccacugg caucguucag     1860

gaaguacaau cgguaaagca uuugaggcca ccgugcgggg cgcuaagcga auggccguuc     1920

ucggggacac agcaugggau uucggaagcg ucggcggugc ucuuaauucc cuggggaaag     1980

gcauccacca gauuuucgga gccgcguuua agucuuuguu cggcgggaug ucgugguuua     2040

gccaaauccu gauuggaacu cuccuggugu ggcuuggccu gaacaccaag aaugguucca     2100

uaucauugac gugucucgcc uuagggggag ugcugaucuu ccugaguacc gcugucucug     2160

cagacguugg cugcagcgug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6210
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 2 opt

<400>  6210
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccguug aagugacgcg acgugguucc gcguacuaua      180

uguaccugga uagaucugac gcuggcgagg ccaucucauu ccccaccaca uuggggguaa      240

auaaaugcua uauucagauc auggauuuag gacacaugug ugacgcaacu augaguuacg      300

aaugcccuau gcuggaugag ggcguggaac cagacgaugu cgacuguugg ugcaacacca      360

caagcaccug ggugguuuau gguacuuguc aucacaagaa aggggaggcc aggcgguccc      420

gcagagcugu gacacuaccg ucucauucga ccaggaagcu ccaaacgcgg ucacagacuu      480

ggcuggagag ccgcgaauac accaagcacc uuauucgagu cgagaauugg aucuuuagaa      540

accccggauu cgcacuggcc gcugccgcga uagcaugguu gcucggcagu uccacaucuc      600

agaaagugau cuaucuggua augauucugc ucaucgcccc ugcuuacagc auuaggugca      660

ucggcguuuc caaucgggau uuuguggaag ggaugucagg agguacuugg gucgacgugg      720

uguuagagca uggcgggugu gucaccguua uggcccagga uaagccaaca guggacauag      780

agcugguaac cacgacugug aguaacaugg cagaaguccg uagcuacugc uaugaggcuu      840

cuauuuccga uauggccuca gacagccgcu gucccacaca aggagaagcg uaccuugaca      900

aacagaguga uacccaguau gugugcaaga gaacuuuggu ugacaggggc ugggguaaug      960

gguguggacu guucggcaaa ggcucucuag ugacaugcgc caaguuugca ugcuccaaga     1020

aaaugaccgg gaaguccauc cagccugaga accucgaaua ccggauuaug cugucggucc     1080

acggaucuca acacuccggu augaucguga augauacggg ccaugagacc gacgagaacc     1140

gagcuaaagu agaaaucaca ccaaauucac cccgcgccga ggcuacucuu gggggauucg     1200

gcagucuggg uuuggauugu gaaccuagaa ccgggcucga cuuuagcgau cuguauuacu     1260

uaacaaugaa caauaagcac uggcugguuc auaaagagug guuccacgac auuccacucc     1320

cguggcacgc cggcgcagau acuggaacac cccauuggaa caauaaggaa gcccuugugg     1380

aguuuaaaga cgcucacgcg aagaggcaga ccgucguggu ccugggcucc caggaggggg     1440

cagugcauac cgccuuggcu ggagcccuag aagcagagau ggauggugcc aagggccggc     1500

ugucuagcgg gcaccucaaa ugccgccuga agauggacaa acuuagacug aagggaguuu     1560

cauauaguuu guguacugcu gccuucacgu uuacaaaaau accugcagaa acccuccaug     1620

gcacugugac cguagagguc caguacgcgg guacagacgg gccaugcaag gugcccgcuc     1680

aaauggccgu ugauaugcag acccugacuc cugugggcag guuaaucaca gcaaacccag     1740

ucauuacgga auccaccgag aacagcaaga ugaugcugga gcuagauccc ccguucggag     1800

acucuuacau cgugauaggc guaggggaca aaaagauuac ucaccacugg uaucguucag     1860

gaaguacaau cgguaaagcc uuugaagcua ccgugcgggg cgccaagcga auggcaguuc     1920

ucggggauac agcuugggac uucggaagcg ucggcggugc ccugaauagc cuugggaaag     1980

gcauccauca gauuuucgga gcggccuuua agucuuuguu cggcgggaug ucgugguuua     2040

gccaaauccu gauuggaacu cuccuggugu ggcuuggccu gaacaccaag aaugguucca     2100

uaucauugac gugucucgcu uuagggggag ugcugaucuu ccugaguacc gcagucucug     2160

ccgauguugg cugcagcgug gacuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6211
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 2 opt

<400>  6211
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag aaaaagcggc gcggcgcuga caccagcgug gggauagucg      120

gacugcuccu uacaacugca auggccguug aagugacgcg acgugguucc gcguacuaua      180

uguaccugga uagaucugac gcuggcaaag ccaucucauu ccccaccaca uuggggguaa      240

auaagugcua uauucagauc auggauuuag gacacaugug ugacgcaacu augaguuacg      300

agugcccuau gcuggaugaa ggcguggagc cagacgaugu cgacuguugg ugcaacacca      360

caagcaccug ggugguuuau gguacuuguc aucacaaaaa gggggaagcc aggcgguccc      420

gcagagcugu gacacuaccg ucucauucga ccaggaagcu ccaaacgcgg ucacagacuu      480

ggcuggagag ccgcgaguac accaaacacc uuauucgagu cgaaaauugg aucuuuagaa      540

accccggauu cgcacuggcc gcugccgcga uagcaugguu gcucggcagu uccacaucuc      600

agaaggugau cuaucuggua augauucugc ucaucgcccc ugcuuacagc auuaggugca      660

ucggcguuuc caaucgggau uuuguggagg ggaugucagg agguacuugg gucgacgugg      720

uguuagaaca uggcgggugu gucaccguua uggcccagga uaaaccaaca guggacauag      780

agcugguaac cacgacugug aguaacaugg cagagguccg uagcuacugc uaugaggcuu      840

cuauuuccga uauggccuca gacagccgcu gucccacaca aggagaagcg uaccuugaca      900

agcagaguga uacccaguau gugugcaaaa gaacuuuggu ugacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag ugacaugcgc caaguuugca ugcuccaaaa     1020

agaugaccgg gaaaagcauc cagccugaaa accucgagua ccggauuaug cugucggucc     1080

acggaucuca acacuccggu augaucguga augauaucgg ccaugaaacg gacgagaacc     1140

gagcuaaggu agagauuacc ccaaauucac cccgcgccga agcuacacuu gggggauucg     1200

gcagucuggg uuuggauugu gagccuagaa cugggcucga cuuuagcgau cuguauuacu     1260

uaaccaugaa caauaaacac uggcugguuc auaaggaaug guuccacgac auaccacucc     1320

cguggcacgc cggcgcagau acaggaacuc cccauuggaa caauaaagag gcccuugugg     1380

aauuuaagga cgcucacgcg aagaggcaga ccgucguggu ccugggcucc caggaggggg     1440

cagugcauac agccuuggcu ggagcccuag aggcagaaau ggauggugcc aaaggccggc     1500

ugucuagcgg gcaccucaag ugccgccuga aaauggacaa gcugagacug aaaggaguuu     1560

cauauaguuu guguaccgcu gccuucacuu uuacgaagau cccugcagag acacuccaug     1620

gcaccgugac uguagaaguc caguacgcgg guaccgacgg gccaugcaag gugcccgcuc     1680

aaauggccgu ugauaugcag acacugaccc cugugggcag guuaauuacu gcaaacccag     1740

ucaucacaga guccacggaa aacagcaaaa ugaugcugga gcuagauccc ccguucggag     1800

acucuuacau agugauuggc guaggggaca agaaaaucac ccaccacugg caucguucag     1860

gaaguacuau cgguaaggcc uuugaggcua cagugcgggg cgccaaacga auggcaguuc     1920

ucggggauac cgcuugggac uucggaagcg ucggcggugc ccuuaauucc cuugggaagg     1980

gcauucacca gaucuucgga gcggccuuua agucuuuguu cggcgggaug ucgugguuua     2040

gccaaauucu gauaggaaca cuccuggugu ggcuuggccu gaacacuaaa aaugguucca     2100

ucucauugac cugucucgcu uuagggggag ugcugauuuu ccugaguacg gcagucucug     2160

ccgauguugg cugcagcgug gacuuuucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6212
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 2 opt

<400>  6212
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucgugg      120

gacugcuccu uacaacugca auggccgcgg aaauuacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caaucucauu cgccaccaca uuggggguca      240

auaaaugcca cguucagauu auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguggagc cugacgaugu agacuguugg ugcaacacca      360

caagcaccug gguggucuac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu gacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuaucaaagu ugaaaauugg auauuuagaa      540

acccgggauu cacacuggug gcugucgcca ucgcgugguu gcucggcagu uccacuucuc      600

agaaggugau uuaccuggua augauccugc ucauugcacc cgccuauagc aucaggugca      660

uaggcguuuc caaucgggau uuuguggagg ggaugucagg agguaccugg gucgacgugg      720

uguuagaaca cggcgggugu gucacaguua uggcucagga uaaaccuacc guggacauug      780

agcugguaac gacuacagug aguaacaugg ccgagguccg uagcuacugc uacgaagcau      840

cuaucuccga uauggcuuca gacagccgcu guccaaccca aggagaggcc uaucuugaca      900

agcagaguga uacucaguac gugugcaaaa gaacauuggu ugacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag ugaccugcgc gaaguuuacg ugcuccaaaa     1020

agaugaccgg gaaaagcauu cagcccgaaa accucgagua ucggaucaug cugucggucc     1080

acggaucuca acauuccggu augaucguga augauacagg ccacgaaacu gacgagaacc     1140

gagccaaggu agagguuacc ccuaauucac cacgcgcaga agcuacacuu gggggauucg     1200

gcagucuggg uuuggauugu gagcccagaa cugggcucga cuuuagcgau cuguacuauu     1260

uaaccaugaa caauaaacau uggcuggugc acaaggaaug guuccacgac auuccucucc     1320

cauggcaugc cggcgcugau acaggaaccc cgcacuggaa caauaaagag gcccuugucg     1380

aauuuaagga cgcacaugcc aagaggcaga cuguggucgu gcugggcucc caggaggggg     1440

cuguucacac ggcguuggca ggagcccuag aggcugaaau ggauggugcc aaaggccggc     1500

uguucucugg gcaucucaag ugccgccuga aaauggacaa acuuagacug aagggaguga     1560

gcuacucauu guguacagca gccuuuaccu ucacuaaggu acccgcugag acccuccacg     1620

gcacagucac cguggaaguu caguaugccg guacugacgg gccuugcaag gugccagcac     1680

aaauggcggu cgauaugcag acacugacgc ccgugggcag guuaauaacc gcuaacccug     1740

uaaucacuga gaguacagaa aacuccaaaa ugaugcugga gcuagaccca cccuuuggag     1800

auagcuacau ugugaucggc guuggggaca agaaaauaac ccaccauugg caccguucug     1860

gaucaacaau ugguaaggcc uucgaggcaa cuguccgggg cgccaaacga auggcugugc     1920

ucggggauac cgccugggac uucggaagug ugggcggugu cuuuaauagc cuggggaagg     1980

gcauccauca gaucuucgga gcagcuuuua agucccuuuu cggcgggaug ucuugguuuu     2040

cgcaaauuuu gaucggaacg cugcucguuu ggcugggccu uaacaccaaa aaugguagca     2100

uuucccugac auguuuggcc cucgggggag ugaugauauu ccugucaacu gcggugagug     2160

ccgauguagg cugcucuguc gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6213
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 2 opt

<400>  6213
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaau uuggggguga      240

acaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuaca augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaauacua      360

ccagcacaug gguaguguac gguaccuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu cacucuacca ucucacucga cacggaagcu ccaaacccgc ucacagacgu      480

ggcuggagag ccgagaguau acuaagcauc uuauaaaagu ggaaaacugg aucuuuagaa      540

auccgggauu cgcucuggcc gcgguugcaa uugccugguu gcucggcagu uccaccucuc      600

agaaggugau cuaccugguc augauucugc ucaucgcucc cgccuauagc auaaggugca      660

uuggcguguc caaccgggau uuuguagagg ggaugucagg agguacaugg guugacgugg      720

ucuuagaaca cggcgggugu gugacuguga uggcacagga uaaaccuacc gucgacaucg      780

agcugguuac aaccacggug aguaauaugg cugagguacg uagcuacugc uacgaagccu      840

cuauuuccga uauggcguca gacagccgcu guccaacuca aggagaggcc uaucuugaca      900

agcagaguga uacacaguac gugugcaaaa gaaccuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaag ggcucucuag ugacuugcgc aaaguuuaca ugcuccaaaa     1020

agaugaccgg gaaaagcauc cagcccgaaa aucucgagua ucggaucaug cugucgguuc     1080

acggaucuca acauuccggu augauuguga acgauauagg ccacgaaacg gacgagaauc     1140

gagcuaaggu cgaggugacc ccuaacucac cacgcgccga agcuacacuu gggggauucg     1200

gcagucuggg uuuggauugu gagcccagaa cugggcucga cuuuagcgau cuguacuauu     1260

uaaccaugaa uaacaaacau uggcugguac acaaggaaug guuccacgac aucccucucc     1320

cauggcaugc cggcgcagau acaggaacuc cgcacuggaa uaacaaagag gcccuuguug     1380

aauuuaagga cgcucaugcg aagaggcaga ccguggucgu gcugggcucc caggaggggg     1440

caguccacac agccuuggcu ggagcccuag aggcagaaau ggauggugcc aaaggccggc     1500

uguucucugg gcaucucaag ugccgccuga aaauggacaa acuuagacug aagggaguga     1560

gcuacucauu guguaccgcu gccuuuacuu ucacgaaggu ucccgcagag acacuccacg     1620

gcaccgugac uguagaaguc caguaugcgg guaccgacgg gccuugcaag gugccagcuc     1680

aaauggccgu ugauaugcag acacugaccc ccgugggcag guuaauuacu gcaaacccug     1740

ucaucacaga gaguacggaa aauuccaaaa ugaugcugga gcuagaccca cccuuuggag     1800

auagcuacau agugauuggc guaggggaca agaaaaucac ccaccauugg caccguucug     1860

gaucaacuau cgguaaggcc uucgaggcua cagugcgggg cgccaaacga auggcaguuc     1920

ucggggauac cgcuugggac uucggaagug ucggcggugu guuuaacagc cuggggaagg     1980

gcgugcauca gauuuucgga gccgcguuua agucccuuuu cggcgggaug ucuugguuuu     2040

cgcaaaucuu gauuggaaca cugcucgucu ggcugggccu uaauacuaaa aacgguagca     2100

uaucccugac cuguuuggcc cucgggggag uuaugaucuu ccugucaacg gcugugagug     2160

cagauguggg cugcucugua gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6214
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 2 opt

<400>  6214
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca uuggggguga      240

auaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu cacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuauaaaagu ggaaaauugg aucuuuagaa      540

acccgggauu cgcucugguu gccguggcga uugcaugguu gcucggcagu uccacaucuc      600

agaaggucau cuaccuggug augauucugc ucaucgcccc cgcuuauagc auaaggugca      660

uuggcguauc caaucgggau uuuguugagg ggaugucagg agguacuugg guggacgucg      720

uguuagaaca cggcgggugu gugaccguca uggcccagga uaaaccuaca guugacaucg      780

agcuggugac cacgacugua aguaacaugg cagaggugcg uagcuacugc uacgaggcuu      840

cuauuuccga uauggccuca gacagccgcu guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gucugcaaaa gaacuuuggu ggacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag uuacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauc cagcccgaaa accucgagua ucggaucaug cugucggugc     1080

acggaucuca acauuccggu augauuguca augauauagg ccacgaaacc gacgagaacc     1140

gagcaaaggu ggagguaaca ccuaauucac cacgcgcuga agccacucuu gggggauucg     1200

gcagucuggg uuuggauugu gagcccagaa ccgggcucga cuuuagcgau cuguacuauu     1260

uaacaaugaa caauaaacau uggcugguuc acaaggaaug guuccacgac aucccucucc     1320

cauggcaugc uggcgccgau acuggaaccc cgcacuggaa caauaaagag gcacuugugg     1380

aauuuaagga cgcccaugcu aagaggcaga cagucguggu ccugggcucc caggaggggg     1440

cggugcacac cgcauuggcc ggagcucuag aggccgaaau ggauggugca aaaggccggc     1500

uguucucugg gcaucucaag ugccgccuga aaauggacaa acuuagacug aagggaguua     1560

gcuacucauu guguacugcc gcuuuuacgu ucacaaaggu gcccgccgag acccuccacg     1620

gcacuguaac cgucgaagug caguaugcag guacagacgg gccuugcaag guuccagcgc     1680

aaauggcugu ggauaugcag acccugacuc ccgucggcag guuaauuaca gccaacccug     1740

ugaucacgga gaguaccgaa aacuccaaaa ugaugcugga gcuagaccca cccuuuggag     1800

auagcuacau aguaauuggc gugggggaca agaaaaucac ucaccauugg caccguucug     1860

gaucaacaau cgguaaggca uucgaggcca ccguucgggg cgcuaaacga auggccgucc     1920

ucggggauac agcaugggac uucggaagug ugggcggugu guuuaauagc cuggggaagg     1980

gcauucauca gaucuucgga gcugccuuua agucccuuuu cggcgggaug ucuugguuuu     2040

cgcaaauuuu gauaggaacu cugcucgucu ggcugggccu uaacaccaaa aaugguagca     2100

ucucccugac guguuuggcg cucgggggag uuaugauuuu ccugucaacc gccgugagug     2160

cugauguggg cugcucugua gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6215
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 2 opt

<400>  6215
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaau uuggggguga      240

acaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuaca augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaauacua      360

ccagcacaug gguaguguac gguaccuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu cacucuacca ucucacucga cacggaagcu ccaaacccgc ucacagacgu      480

ggcuggagag ccgagaguau acuaagcauc uuauaaaagu ggaaaacugg aucuuuagaa      540

auccgggauu cgcucuggcc gcgguugcaa uugccugguu gcucggcagu uccaccucuc      600

agaaggugau cuaccugguc augauucugc ucaucgcucc cgccuauagc auaaggugca      660

uuggcguguc caaccgggau uuuguagagg ggaugucagg agguacaugg guugacgugg      720

ucuuagaaca cggcgggugu gugacuguga uggcacagga uaaaccuacc gucgacaucg      780

agcugguuac aaccacggug aguaauaugg cugagguacg uagcuacugc uacgaagccu      840

cuauuuccga uauggcguca gacagccgcu guccaacuca aggagaggcc uaucuugaca      900

agcagaguga uacacaguac gugugcaaaa gaaccuuggu cgacaggggc ugggguaacg      960

gguguggacu guucggcaag ggcucucuag ugacuugcgc aaaguuuaca ugcuccaaaa     1020

agaugaccgg gaaaagcauc cagcccgaaa aucucgagua ucggaucaug cugucgguuc     1080

acggaucuca acauuccggu augauuguga acgaugaaaa ucgagcuaag gucgagguga     1140

cgccuaacuc accacgcgcc gaggcuaccc uuggcggguu cggaagucug ggcuuggacu     1200

gugaacccag aacaggucuc gauuuuagcg accuguacua uuuaacuaug aauaacaaac     1260

acuggcuggu acauaaggag ugguuccacg auauaccucu cccauggcac gccggggcag     1320

acaccggcac accgcauugg aauaacaaag aagcccuugu ugaguuuaag gaugcucacg     1380

cgaagaggca gacugugguc gugcugggau cccaggaagg cgcaguccau accgccuugg     1440

cuggggcccu agaggcagag auggacggag ccaaaggucg gcuguucucu ggccaccuca     1500

agugccgccu gaaaauggau aaacuuagac ugaaaggggu gagcuacuca uuguguacag     1560

cugccuuuac cuucacuaag guucccgcag aaacgcucca uggaacagug accguagagg     1620

uccaguaugc gggcacugac gguccuugca aggugccagc ucaaauggcc guugacaugc     1680

agacccugac acccgugggg agguuaauca ccgcaaaccc ugucauuacu gaaaguacag     1740

aaaauuccaa aaugaugcug gagcuagauc cacccuuugg cgacagcuac aucgugauag     1800

gaguaggcga uaagaaaauu acgcaccacu ggcaucguuc ugggucaacc aucggaaagg     1860

ccuucgaggc uacugugcgg ggugccaaac gaauggcagu ucucggcgac acagcuuggg     1920

auuucgggag ugucggaggc guguuuaaca gccuggguaa ggggauccac cagauuuucg     1980

gcgccgcguu uaagucccuu uucggaggca ugucuugguu uucgcaaauc uugauuggga     2040

cccugcucgu guggcuggga cuuaauacaa agaacggcag cauaucccug acuuguuugg     2100

cccucggugg ggucaugauc uuccugucaa ccgcuguuag ugcagacgug ggaugcucug     2160

uggauuuuag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6216
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 2 opt

<400>  6216
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucgugg      120

gacugcuccu uacaacugca auggccgcgg aaauuacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caaucucauu cgccaccaca uuggggguca      240

auaaaugcca cguucagauu auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguggagc cugacgaugu agacuguugg ugcaacacca      360

caagcaccug gguggucuac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu gacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuaucaaagu ugaaaauugg auauuuagaa      540

acccgggauu cgcucuggug gccgucgcga ucgcaugguu gcucggcagu uccacaucuc      600

agaaggugau uuaccuggua augauccugc ucauugcccc cgcuuauagc aucaggugca      660

uaggcguuuc caaucgggau uuuguggagg ggaugucagg agguacuugg gucgacgugg      720

uguuagaaca cggcgggugu gucaccguua uggcccagga uaaaccuaca guggacauug      780

agcugguaac cacgacugug aguaacaugg cagagguccg uagcuacugc uacgaggcuu      840

cuaucuccga uauggccuca gacagccgcu guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gugugcaaaa gaacuuuggu ugacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag ugacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauu cagcccgaaa accucgagua ucggaucaug cugucggucc     1080

acggaucuca acauuccggu augaucguga augauaccgg ccacgaaaca gacgagaacc     1140

gagcaaaggu agagguuacu ccuaauucac cacgcgcuga agccacccuu gggggauucg     1200

gcagucuggg uuuggauugu gagcccagaa cagggcucga cuuuagcgau cuguacuauu     1260

uaacuaugaa caauaaacau uggcuggugc acaaggaaug guuccacgac auuccucucc     1320

cauggcaugc uggcgccgau accggaacac cgcacuggaa caauaaagag gcacuugucg     1380

aauuuaagga cgcccaugcu aagaggcaga ccguggucgu gcugggcucc caggaggggg     1440

cgguucacac ugcauuggcc ggagcucuag aggccgaaau ggauggugca aaaggccggc     1500

uguucucugg gcaucucaag ugccgccuga aaauggacaa acuuagacug aagggaguga     1560

gcuacucauu guguacggcc gcuuuuacau ucaccaaggu acccgccgag acucuccacg     1620

gcaccgucac aguggaaguu caguaugcag guaccgacgg gccuugcaag gugccagcgc     1680

aaauggcugu cgauaugcag acucugacac ccgugggcag guuaauaacg gccaacccug     1740

uaaucaccga gaguacugaa aacuccaaaa ugaugcugga gcuagaccca cccuuuggag     1800

auagcuacau ugugaucggc guuggggaca agaaaauaac acaccauugg caccguucug     1860

gaucaaccau ugguaaggca uucgaggcca caguccgggg cgcuaaacga auggccgugc     1920

ucggggauac ugcaugggac uucggaagug ugggcggugu cuuuaauagc cuggggaagg     1980

gcauccauca gaucuucgga gcugccuuua agucccuuuu cggcgggaug ucuugguuuu     2040

cgcaaauuuu gaucggaacc cugcucguuu ggcugggccu uaacacgaaa aaugguagca     2100

uuucccugac cuguuuggcg cucgggggag ugaugauauu ccugucaaca gccgugagug     2160

cugauguagg cugcucuguc gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6217
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 2 opt

<400>  6217
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucgugg      120

gacugcuccu uacaacugca auggccgcgg aaauuacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caaucucauu cgccaccaca uuggggguca      240

auaaaugcca cguucagauu auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguggagc cugacgaugu agacuguugg ugcaacacca      360

caagcaccug gguggucuac gguacuuguc aacacaagaa aggggaagca cggcgcucca      420

gaagggccgu gacacuacca ucucauucga cccggaagcu ccagacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcacc uuaucaaagu ugaaaauugg auauuuagaa      540

acccgggauu cgcucuggug gccgucgcga ucgcaugguu gcucggcagu uccacaucuc      600

agaaggugau uuaccuggua augauccugc ucauugcccc cgcuuauagc aucaggugca      660

uaggcguuuc caaucgggau uuuguggagg ggaugucagg agguacuugg gucgacgugg      720

uguuagaaca uggcgggugu gucaccguua uggcccaaga uaaaccuaca guggacauug      780

agcugguaac cacgacugug aguaacaugg cagagguccg uagcuacugc uacgaggcuu      840

cuaucuccga uauggccuca gacagccgcu guccaacaca gggagaagcg uaucuugaca      900

agcagaguga uacccaguac gugugcaaaa gaacuuuggu ugacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag ugacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauu caacccgaaa accucgagua ucggaucaug cugucggucc     1080

acggaucuca gcacuccggu augaucguga augauauugg ccaugaaacc gacgagaacc     1140

gagcaaaggu agagguuaca ccuaauucac cacgcgcuga agccacucuu gggggauucg     1200

gcagucuggg uuuggauugu gagcccagaa ccgggcucga cuuuagcgau cuguacuauu     1260

uaacaaugaa caauaaacac uggcuggugc auaaggaaug guuccacgac auaccucucc     1320

cauggcacgc uggcgccgau acuggaaccc cgcauuggaa caauaaagag gcacuugucg     1380

aauuuaagga cgcccacgcu aagaggcaga caguggucgu gcugggcucc caggaggggg     1440

cgguucauac cgcauuggcc ggagcucuag aggccgaaau ggauggugca aaaggccggc     1500

uguucucugg gcaccucaag ugccgccuga aaauggacaa acuuagacug aagggaguga     1560

gcuacucauu guguacugcc guaugcacgg cugccaaggu ccccgcagag acacuccaug     1620

gcaccgugac uguugaagug caauaugcgg guaccgacgg gccuuguaag gucccagcuc     1680

agauggccgu ggauaugcag acacugaccc ccguaggcag guuaaucacu gcaaacccug     1740

ugauuacaga gaguacggaa aacuccaaaa ugaugcugga gcuagaccca cccuuuggag     1800

auagcuacau cguuauaggc gucggggaca agaaaauuac ccaccacugg caucguucug     1860

gaucaacuau cgguaaggcc uucgaggcua cagugcgggg cgccaaacga auggcagugc     1920

ucggggauac cgcuugggac uuuggaagug ucggcggugu uuucaauagc cuggggaagg     1980

gcauccacca aauuuucgga gccgcguuua agucccuuuu cggcgggaug ucuugguuuu     2040

cgcagaucuu gauuggaaca cugcucgugu ggcugggccu uaacacuaaa aaugguagca     2100

uaucccugac cugcuuggcc cucgggggag ugaugaucuu ccugucaacg gcuguaagug     2160

cagaugucgg cuguucugug gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6218
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 2 opt

<400>  6218
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga cgcaagcaua gggaucgugu      120

cccugcuccu uaccacaguc auggccgcgg aaauuacucg uagaggaucu gcuuacuaua      180

uguaccugga uaggucagac gccgguaagg caaucaguuu cguuacgacc uugggcguga      240

auaaaugcca cguacagauu auggauuuag ggcauaugug ugacgccaca augagcuaug      300

agugccccau gcuggaugaa ggaguggagc cugacgaugu cgacuguugg ugcaacacua      360

ccuccacaug ggugguuuac ggcaccuguc accauaagaa aggugaagcu cggcgcucua      420

gaagggcagu gacucuacca ucgcacucaa cacggaagcu ccaaacccgc agccagacgu      480

ggcuggagag ucgagaguau acuaagcauc uuaucaaagu cgaaaauugg auauuuagaa      540

acccgggguu cgcccuggug gcuguagcca ucgcgugguu gcucggaucc ucuaccagcc      600

agaagguuau uuaccuggug augauccugc ucauugcacc cgccuauucc aucaggugca      660

uaggcgucuc aaaucgggau uuuguggagg gcaugagugg gggaacaugg guggacgucg      720

uuuuagaaca cgguggcugu gugacuguaa uggcucagga uaaaccuacc guggacauug      780

agcuggucac aaccacggug agcaacaugg ccgagguucg uucuuacugc uacgaagcau      840

ccaucucaga uauggcuagc gacagucgcu guccaacuca aggggaggcc uaucuugaca      900

agcagucuga uacacaguac gugugcaaaa gaaccuuggu cgacagggga uggggcaaug      960

guugugggcu guucggaaag ggcucccuag ugacuugcgc gaaguuuaca ugcagcaaaa     1020

agaugaccgg caaaucgauu cagcccgaaa accucgagua ucggaucaug cugucuguac     1080

acggguccca acauucagga augaucguua augaugaaaa ccgagccaag guggagguca     1140

cgccuaauag uccacgcgca gaggcuaccc uugguggcuu cgggagccug ggauuggacu     1200

gugaacccag aacaggccuc gauuuuuccg accuguacua uuuaacuaug aacaauaaac     1260

acuggcuggu gcauaaggag ugguuccacg auauuccucu cccauggcac ucuggugccg     1320

acaccgaaac accgcauugg aacaauaaag aggcucuugu cgaauuuaag gaugcccacg     1380

caaagaggca gacugugguu gugcugggga gccaggaggg cgccguacau accgcuuugg     1440

cgggagcacu agaggccgaa auggacggcg cuaaagggcg gcugucaagu ggacaccuca     1500

agugccgccu gaaaauggau aaacuuagac ugaaaggugu cuccuacagc uuguguacag     1560

ccgcauucac cuuuacuaag gugcccgccg agacgcucca uggcacaguu accguggaag     1620

uccaguaugc ugggagggac ggaccuugca aggugccagc ccaaauggca guagacaugc     1680

agacucugac ccccgugggc cguuuaauaa cagcgaaccc uguuaucacc gagucuacug     1740

aaaacucaaa aaugaugcug gagcuagauc cacccuucgg ugacaguuac auugucaucg     1800

gggugggcga uaagaaaaua acacaccacu ggcaucggag cggauccauu aucggcaagg     1860

cuuuugaggc cacggugcga ggggcaaaaa gaauggccgu ccucggagac accgcuuggg     1920

auuucgguuc uguuggcggg guguucaauu cgcugggaaa gggcauccac cagauuuuug     1980

gugccgcauu caagagccuu uuugggggca uguccugguu cucacaaauc uugauuggaa     2040

cucugcucgu guggcugggc cuuaacacaa agaaugggag uauaucucug accuguuugg     2100

cucucggagg cguaaugauc uuucugagca cagccgucuc cgcggacgug gguugcucag     2160

uugauuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6219
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 2 opt

<400>  6219
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucgugg      120

gacugcuccu uacaacugca auggccgcgg aaauuacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caaucucauu cgccaccaca uuggggguca      240

auaaaugcca cguucagauu auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguggagc cugacgaugu agacuguugg ugcaacacca      360

caagcaccug gguggucuac gguacuuguc accauaagaa aggggaagca cggcgcucca      420

gaagggccgu gacacuacca ucucacucga cccggaagcu ccaaacgcgc ucacagacuu      480

ggcuggagag ccgagaguau accaagcauc uuaucaaagu ugaaaauugg auauuuagaa      540

acccgggauu cgcucuggug gccgucgcga ucgcaugguu gcucggcagu uccacaucuc      600

agaaggugau uuaccuggua augauccugc ucauugcccc cgcuuauagc aucaggugca      660

uaggcguuuc caaucgggau uuuguggagg ggaugucagg agguacuugg gucgacgugg      720

uguuagaaca cggcgggugu gucaccguua uggcccagga uaaaccuaca guggacauug      780

agcugguaac cacgacugug aguaacaugg cagagguccg uagcuacugc uacgaggcuu      840

cuaucuccga uauggccuca gacagccgcu guccaacaca aggagaagcg uaucuugaca      900

agcagaguga uacccaguac gugugcaaaa gaacuuuggu ugacaggggc ugggguaaug      960

gguguggacu guucggcaag ggcucucuag ugacaugcgc caaguuuacc ugcuccaaaa     1020

agaugacggg gaaaagcauu cagcccgaaa accucgagua ucggaucaug cugucggucc     1080

acggaucuca acauuccggu augaucguga augaugaaaa ccgagcaaag guagagguua     1140

ccccuaauuc accacgcgcu gaggccacac uuggcggguu cggaagucug ggcuuggacu     1200

gugaacccag aacuggucuc gauuuuagcg accuguacua uuuaaccaug aacaauaaac     1260

acuggcuggu gcauaaggag ugguuccacg auauuccucu cccauggcac gcuggggccg     1320

acacaggcac uccgcauugg aacaauaaag aagcacuugu cgaguuuaag gaugcccacg     1380

cuaagaggca gaccgugguc gugcugggau cccaggaagg cgcgguucau acagcauugg     1440

ccggggcucu agaggccgag auggacggag caaaaggucg gcuguucucu ggccaccuca     1500

agugccgccu gaaaauggau aaacuuagac ugaagggggu gagcuacuca uuguguaccg     1560

ccgcuuuuac uuucacgaag guacccgccg aaacacucca uggaaccguc acuguggagg     1620

uucaguaugc aggcaccgac gguccuugca aggugccagc gcaaauggcu gucgacaugc     1680

agacacugac ccccgugggg agguuaauaa cugccaaccc uguaaucaca gaaaguacgg     1740

agaacuccaa aaugaugcug gaacuagauc cacccuuugg cgacagcuac auugugaucg     1800

gaguuggcga uaagaaaaua acccaccacu ggcaucguuc ugggucaacu auuggaaagg     1860

cauucgaggc cacaguccgg ggugcuaaac gaauggccgu gcucggcgac accgcauggg     1920

auuucgggag ugugggaggc gucuuuaaua gccuggguaa ggggauccac cagaucuucg     1980

gcgcugccuu uaagucccuu uucggaggca ugucuugguu uucgcaaauu uugaucggga     2040

cacugcucgu uuggcuggga cuuaacacua aaaauggcag cauuucccug accuguuugg     2100

cgcucggugg ggugaugaua uuccugucaa cggccgugag ugcugacgua ggaugcucug     2160

ucgauuuuag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6220
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 2 opt

<400>  6220
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca uuggggguga      240

auaaaugcca cguccagauc auggauuuag gacauaugug ugacgcuacu augaguuaug      300

agugccccau gcuggaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accauaagaa aggggaaaca cggcgcucca      420

gaaggucugu cucgcuacgg uaucacuaca cccgcaagcu ccaaacgcga ucacagacuu      480

ggcuggagag cagagaguau aagaaacauc uuauaauggu ggaaaauugg aucuuuagga      540

acccaggauu cgcaauuguu aguguggcca ucaccuggcu gaugggcucc uugacaucuc      600

agaaggucau uuaccucgug augaucguac ugauaguucc ggcuuacagc auuuccugca      660

ucggcguguc aaaucgggau cuggucgagg ggaugagugg agguacuugg guggacgugg      720

uccucgaaca cggcgggugu guuaccgaga uggcccagga uaaacccaca guggacauug      780

aguuaguaac caugacggug agcaacaugg cggaaguccg uucuuauugc uacgaggcau      840

cccugucaga uauggccagc gcuagucgcu guccuacuca aggagaacca ucucuugaca      900

agcaguccga cacacagagc gugugcaaaa gaaccuuggg cgauaggggu ugggggaaug      960

gauguggcau cuuuggcaag gggucgcugg uuacuugcuc uaaguucaca ugcuguaaaa     1020

agaugcccgg aaaauccauc cagccugaga accuagaaua ucggauuaug cucccagugc     1080

acgguucaca acauaguggc augauaguca augacaucgg gcacgagacc gaugagaacc     1140

gagccaaggu ggaaguaacg cccaauagcc cucgcgcaga ggcuacccug ggaggcuuug     1200

guucccuugg gcuggacugc gaaccaagaa caggcuugga uuucucugac cucuacuauc     1260

ugacuaugaa caauaaacau ugguuaguuc acaaggagug guuucacgau auuccgcugc     1320

ccuggcaugc cggagcggac accggcacac cucacuggaa caauaaagaa gcccucgugg     1380

aguucaagga ugcacaugcu aagaggcaga cugucguggu ccuugggagc caggagggag     1440

ccgugcacac cgcucuggcc ggugcauugg aagccgagau ggacggcgcu aaagggcggc     1500

uauuuucagg acaucugaag ugucgccuca aaauggauaa gcugagacuu aaaggcguua     1560

guuacucccu gugcacagcg gcauucaccu uuacuaaggu gccagccgaa acguugcacg     1620

guacaguaac cgucgaggug cagagcgcug ggacugacgg ccccuguaag guuccugccc     1680

aaauggcagu ggacaugcag acccucacac cagucggaag gcugaucacc gccaaccccg     1740

ugauaacuga aucuacagag aacucaaaaa ugauguuaga gcuggauccg ccuuucggcg     1800

acaguuauau uguaaucggg gugggagaua agaaaaucac gcaccauugg caccguagcg     1860

guuccaccau uggcaaggcu uuugaagcca cuguucgagg ggcaaaacgg auggcggucc     1920

uaggagacac agcuugggau uucggcucug uggguggggu guucaauucg cucggcaagg     1980

gaauccauca gauuuuuggc gccgcauuca agagccuguu ugggggaaug uccugguucu     2040

cacaaauacu uaucggcacc uugcuggucu ggcucggucu gaacacuaaa aaugggagua     2100

uuucucuuac augccuggcc uugggaggcg uuaugaucuu ucucagcacc gcuguguccg     2160

ccgacguggg uuguucagua gauuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6221
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 2 opt

<400>  6221
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc caggaaggag cggaaacgcc gaggcgcuga caccagcaua gggaucauug      120

gacugcuccu uacaacugca auggccgcgg aaaucacgcg uagagguucc gcuuacuaua      180

uguaccugga uaggucugac gccggcaagg caauuucauu cgccaccaca auggggguga      240

auaaaugcca cguccagauc auggauuugg gacauaugug ugacgcuacu augaguuaug      300

agugccccau guuagaugaa ggcguugagc cugacgaugu ggacuguugg ugcaacacca      360

caagcaccug gguaguguac gguacuuguc accauaagaa aggggaagca cggccauccc      420

gcagagccgu cacacugccg ucucacucga ccaggaagcu acaaacgcgg ucacagacuu      480

ggcucgagag ccgcgaguau accaagcauc ugauaaaagu ggaaaauugg auccuucgaa      540

accccggauu uacaguugcu gcccuggcga uggcaugguu gcucggcaau aguacuuccc      600

agagagugau uuaccugguc augauccugc ucauugcccc ugcuuauucu aucaggugca      660

uaggcgugag caaccgggau uucguagagg ggauguccgg agguaccugg guugacgugg      720

ucuuagaaca cggcgggugu gugacaguga uggcccagga uaagccaacc gucgacauug      780

agcugguuac gacuacagug ucaaauaugg cagagguacg uaguuacugc uacgaagcua      840

gcaucucuga uauggccucc gacucacgcu gucccaccca aggagaggcg uaucuugaca      900

aacagagcga uacucaguac gugugcaaga gaacauuggu cgacaggggc ugggguaacg      960

gguguggacu guuuggcaaa ggcagucuag ugaccugcgc caaguucacg ugcucuaaga     1020

aaaugaccgg gaaguccauu cagccugaaa aucucgagua ucggaucaug cugagcguuc     1080

acggaucgca acauucuggu augaucguga acgauauugg ccacgaaaca gacgagaauc     1140

gagcaaaagu cgaggugacu ccaaacucac cccgcgcuga agccacccuu gggggauuug     1200

gcucccuggg uuuggauugu gagccuagaa cagggcucga cuucagugau cuguacuauu     1260

uaacuaugaa uaacaagcau uggcugguac acaaagaaug guuucacgac auaccacucc     1320

cguggcaugc uggcgccgau accggaacac cccacuggaa uaacaaggag gcacuuguug     1380

aguucaaaga cgcccaugcu aagaggcaga ccguggucgu gcugggcagc caggaagggg     1440

cgguccacac ugcauuggcc ggagcucuag aggccgaaau ggauggugca aagggccggc     1500

uguuuuccgg gcaucucaaa ugccgcauca agauggacaa acugagacuu aagggagugu     1560

cuuacagccu guguacggcc gcuuucacau uuucaaaagu uccugccgag accuugcacg     1620

gcacugugac cguagaaguc caguaugcag guagggacgg gccaugcaag gugcccgcgc     1680

aaauggcugu ugauaugcag acacucaccc cugugggccg ucugauuacu gccaacccaa     1740

ucauaacaga gaguacggaa aauucccgga ugauguuaga gcuggauccc ccguucggag     1800

acagcuacau ugucaucggc gugggggaca agaaaaucac ccaccauugg caccgaucug     1860

gaucaacuau ugguaaggca uuugaggcca caguaagagg cgcuaaaagg auggccgugc     1920

uaggggauac cgcaugggac uucggaagug uuggcggugu cuucaacagc cucgggaagg     1980

gcgugcauca gguguuugga gcugccuuca aaucccuguu uggcgggaug ucuugguucu     2040

cgcaaauccu uauuggaaca uugcuggucu ggcucggccu gaauacuaag aacgguagca     2100

uaucccuugc gugucuggcc cucgggggag cuaugaucuu uuugucaacc gcaguuagug     2160

ccgauguggg cugcucugug gacuucugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  6222
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 2 opt

<400>  6222
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uauuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6223
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 2 opt

<400>  6223
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac acuugugaug      240

ccaccauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa cacaaccagu acuugggugg ucuauggcac augucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugaccc uaccguccca uucuacgagg aaacuccaaa      420

cucggucgca gaccuggcug gagucacgcg aguacacaaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac uucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

ccuggguuga uguggucuua gaacauggug gcugugugac agugauggcu caggacaagc      720

caaccgucga uauugagcug guuacgacua cagugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acccaagggg      840

aggccuaccu ugacaagcag agcgacacuc aguaugugug caaaaggaca uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugacc ugcgcgaaau      960

uugccugcuc uaagaaaaug acgggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac accggucaug     1080

aaacagauga gaaccgcgca aaggucgaga uuacuccaaa uucccccaga gcugaagcca     1140

cccuuggcgg guucggauca cugggcuugg acugugagcc uaggacaggu cucgauuuua     1200

gugaccugua uuacuuaacu augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacaccgg cacaccccau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagaccguu gugguccugg     1380

gaagccagga gggcgcggug cauacugcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cggccgcuuu cacauuuacc aagaucccug     1560

ccgagacucu ccauggaacc gugacaguug aagugcagua cgcaggcacc gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacucu gacaccugug gggagguuaa     1680

uuacggccaa cccaguuaua accgagagua cugaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacacacc     1800

acuggcaucg gucuggguca accauaggaa aggcauuuga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauacugcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacccuccu gauguggcuu ggacugaacg     2040

ccaagaaugg cagcaucucc uugauguguc ucgcucuggg ugggguuuua auuuuccugu     2100

caacggcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6224
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 2 opt

<400>  6224
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauu aagguggaga      480

acuggaucuu uagaaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauuag gugcaucggc guauccaacc gggacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcacggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgcagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaagaacu uugguggaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauccagcc cgaaaaucuc gaauaucgga     1020

uuaugcuguc ggugcacggg ucucaacauu ccggaaugau cgguuacgag accgacgaag     1080

aucgugcgaa ggucgaggug acaccuaacu caccacgcgc cgaggcaacu cuuggcgggu     1140

ucggaagucu gggcuuggac ugugaaccca gaaccggucu cgauuuuagc gaccuguauu     1200

acuuaacaau gaauaacaaa cacuggcugg uacauaagga gugguuccac gauaucccuc     1260

ucccauggca cgcuggggcc gacacuggca ccccgcauug gaauaacaaa gaagcucuug     1320

uugaguuuaa ggaugcccac gcaaaaaggc agacaguggu cgugcuggga ucccaggaag     1380

gcgccgucca uaccgcuuug gcgggggcac uagaggccga gauggacgga gcuaaggguc     1440

ggcuguucuc uggccaccuc aagugccgac ugaaaaugga uaaacuucgc cugaaggggg     1500

ugagcuauuc auuguguacu gccgcauuua cguucacaaa gguucccgcc gaaacccucc     1560

auggaacugu gaccguagag guccaguacg cuggcacaga cgguccuugc aaaauuccag     1620

ugcaaauggc cguugacaug cagacccuga cucccguggg gagauuaaua acagcaaauc     1680

cugucaucac ggaaaguacc gagaacucca agaugaugcu ggaacuagau ccacccuuug     1740

gcgacagcua cauugugauc ggaguaggcg auaagaaaau aacucaccac uggcauaggu     1800

cugggucaac aauuggaaag gcguucgagg cuaccgugcg gggugccaaa cgcauggcag     1860

uucucggcga cacagccugg gauuucggga gugucggagg cguguuuaau agccugggua     1920

aggggaucca ccagaucuuc ggcgcugccu uuaaaucccu uuucggaggc augucuuggu     1980

uuucgcaaau uuugaucggg acucugcucg uguggcuggg acuuaacacc aagaacggca     2040

gcauuucccu gacguguuug gcacucggug gggucaugau auuccuguca accgcuguua     2100

gugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6225
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 2 opt

<400>  6225
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6226
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 2 opt

<400>  6226
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauu aagguggaga      480

acuggaucuu uagaaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauuag gugcaucggc guauccaacc gggacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcacggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgcagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaagaacu uugguggaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauccagcc cgaaaaucuc gaauaucgga     1020

uuaugcuguc ggugcacggg ucucaacauu ccggaaugau cgucaacgac accgguuacg     1080

agacagauga aaaucgugcg aagguggagg uaacuccuaa cucaccacgc gccgaggcaa     1140

cccuuggcgg guucggaagu cugggcuugg acugugaacc cagaacaggu cucgauuuua     1200

gcgaccugua uuacuuaacu augaauaaca aacacuggcu gguucauaag gagugguucc     1260

acgauauccc ucucccaugg cacgcugggg ccgacaccgg cacaccgcau uggaauaaca     1320

aagaagcucu uguggaguuu aaggaugccc acgcaaaaag gcagaccguc gugguccugg     1380

gaucccagga aggcgccgug cauacugcuu uggcgggggc acuagaggcc gagauggacg     1440

gagcuaaggg uaagcuguuc ucuggccacc ucaaaugccg gcugaagaug gauaaacuuc     1500

gacugaaggg gguuagcuau ucauugugua cggccgcauu uacauucacc aaagugcccg     1560

ccgaaacucu ccauggaacc guaacagucg aggugcagua cgcuggcacc gacgguccuu     1620

gcaagauucc aguucaaaug gccguggaca ugcagacucu gacacccguc gggcgcuuaa     1680

uaacggcaaa uccugugauc accgaaagua cugagaacuc caagaugaug cuggaacuag     1740

auccacccuu uggcgacagc uacauuguaa ucggaguggg cgauaaaaag auaacacacc     1800

acuggcauag aucuggguca accauuggaa aagcguucga ggcuacaguu aggggugcca     1860

agcggauggc aguccucggc gacacugccu gggauuucgg gaguguggga ggcguguuua     1920

acagccuggg uaaagggauc caccagaucu ucggcgcugc cuuuaagucc cuuuucggag     1980

gcaugucuug guuuucgcaa auuuugaucg ggacccugcu cgucuggcug ggacuuaaua     2040

cgaagaacgg cagcauuucc cugaccuguu uggcacucgg ugggguuaug auauuccugu     2100

caacagcugu gagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6227
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 2 opt

<400>  6227
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccgc aagaaaggug      360

aggcccgacg uuccagaagg gcugucacac uaccaucuca uucgacccgg aagcuccaaa      420

cgcgcucaca gacuuggcug gaaagcagag aguauaccaa acaccuuauu aagguggaga      480

acuggaucuu uaggaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauucg gugcaucggc guauccaacc gcgacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcauggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgaagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag cagaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaaggacu uugguggauc      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauccagcc cgaaaaucuc gaauaucgua     1020

uuaugcuguc ggugcacggg ucucaacacu ccggaaugau cgucaacgac accgguuacg     1080

agacagauga aaaucgcgcg aagguggagg uaacuccuaa cucaccaaga gccgaggcaa     1140

cccuuggcgg guucggaagu cugggcuugg acugugaacc caggacaggu cucgauuuua     1200

gcgaccugua uuacuuaacu augaauaaca aacauuggcu gguucacaag gagugguucc     1260

augauauccc ucucccaugg cacgcugggg ccgacaccgg cacaccgcac uggaauaaca     1320

aagaagcucu uguggaguuu aaggaugccc augcaaaacg gcagaccguc gugguccugg     1380

gaucccagga aggcgccgug cacacugcuu uggcgggggc acuagaggcc gagauggacg     1440

gagcuaaggg uaagcuguuc ucuggccauc ucaaaugccg acugaagaug gauaaacuuc     1500

gccugaaggg gguuagcuau ucauugugua cggccgcauu uacauucacc aaagugcccg     1560

ccgaaacucu ccacggaacc guaacagucg aggugcagua cgcuggcacc gacgguccuu     1620

gcaagauucc aguucaaaug gccguggaca ugcagacucu gacacccguc gggagauuaa     1680

uaacggcaaa uccugugauc accgaaagua cugagaacuc caagaugaug cuggaacuag     1740

auccacccuu uggcgacagc uacauuguaa ucggaguggg cgauaaaaag auaacacauc     1800

acuggcacag gucuggguca accauuggaa aagcguucga ggcuacaguu cggggugcca     1860

agcgcauggc aguccucggc gacacugccu gggauuucgg gaguguggga ggcguguuua     1920

acagccuggg uaaagggauc caucagaucu ucggcgcugc cuuuaagucc cuuuucggag     1980

gcaugucuug guuuucgcaa auuuugaucg ggacccugcu cgucuggcug ggacuuaaua     2040

cgaagaacgg cagcauuucc cugaccuguu uggcacucgg ugggguuaug auauuccugu     2100

caacagcugu gagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6228
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 2 opt

<400>  6228
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauu aagguggaga      480

acuggaucuu uagaaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauuag gugcaucggc guauccaacc gggacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcacggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgcagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaagaacu uugguggaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauccagcc cgaaaaucuc gaauaucgga     1020

uuaugcuguc ggugcacggg ucucaacauu ccggaaugac cgucaacgac aucgguuacg     1080

agacagauga aaaucgugcg aagguggagg uaacuccuaa cucaccacgc gccgaggcaa     1140

cccuuggcgg guucggaagu cugggcuugg acugugaacc cagaacaggu cucgauuuua     1200

gcgaccugua uuacuuaacu augaauaaca aacacuggcu gguucauaag gagugguucc     1260

acgauauccc ucucccaugg cacgcugggg ccgacaccgg cacaccgcau uggaauaaca     1320

aagaagcucu uguggaguuu aaggaugccc acgcaaaaag gcagaccguc gugguccugg     1380

gaucccagga aggcgccgug cauacugcuu uggcgggggc acuagaggcc gagauggacg     1440

gagcuaaggg uaagcuguuc ucuggccacc ucaaaugccg gcugaagaug gauaaacuuc     1500

gacugaaggg gguuagcuau ucauugugua cggccgcauu uacauucacc aaagugcccg     1560

ccgaaacucu ccauggaacc guaacagucg aggugcagua cgcuggcacc gacgguccuu     1620

gcaagauucc aguucaaaug gccguggaca ugcagacucu gacacccguc gggcgcuuaa     1680

uaacggcaaa uccugugauc accgaaagua cugagaacuc caagaugaug cuggaacuag     1740

auccacccuu uggcgacagc uacauuguaa ucggaguggg cgauaaaaag auaacacacc     1800

acuggcauag aucuggguca accauuggaa aagcguucga ggcuacaguu aggggugcca     1860

agcggauggc aguccucggc gacacugccu gggauuucgg gaguguggga ggcguguuua     1920

acagccuggg uaaagggauc caccagaucu ucggcgcugc cuuuaagucc cuuuucggag     1980

gcaugucuug guuuucgcaa auuuugaucg ggacccugcu cgucuggcug ggacuuaaua     2040

cgaagaacgg cagcauuucc cugaccuguu uggcacucgg ugggguuaug auauuccugu     2100

caacagcugu gagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6229
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 2 opt

<400>  6229
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccuuacaa cugccauggc ugcagagaua acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauc ucauucgcca      180

ccacauuggg cgucaacaaa ugccacguuc agauuaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ggaaccugau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugugacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauc aagguugaga      480

acuggauuuu uagaaauccg ggguucacac ugguggcagu cgccaucgcu ugguugcucg      540

gaaguuccac uucucagaag gugauauacc ugguaaugau ccugcucauu gcccccgcgu      600

auagcaucag gugcauuggc guuuccaacc gggacuuugu ggaaggcaug ucagggggaa      660

ccugggucga ugugguguua gagcacggug gcugugucac aguuauggca caggacaaac      720

cuaccgugga uaucgaacug guaacgacua cagugaguaa uauggccgag guccgcagcu      780

acugcuacga ggcuucuaua uccgacaugg ccucagauag ccgaugucca acccaagggg      840

aagcauaucu ugacaagcag agugacacuc aguacgugug caaaagaaca uugguugaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugacc ugcgcuaaau      960

uuacgugcuc caagaagaug accggcaaaa gcauucagcc cgagaaucuc gaauaucgga     1020

ucaugcuguc gguccacggg ucucaacauu ccggaaugau ugugaacgac gagaaucgug     1080

ccaagguaga aguuacaccu aacucaccac gcgcggaggc cacucuuggu ggcuucggga     1140

gucugggauu ggauugugag cccagaaccg gccucgacuu uagcgaucug uacuauuuaa     1200

caaugaauaa caaacacugg cuggugcaua aggaaugguu ccacgacauc ccucucccau     1260

ggcacgcagg ggcugauacu gggaccccgc auuggaauaa caaagaggcc cuugucgaau     1320

uuaaggacgc ucacgccaaa aggcagacag uggucgugcu gggcucccag gagggagcag     1380

uucauaccgc cuuggcuggc gcgcuagaag cagagaugga uggggccaag ggacggcugu     1440

ucucugguca ccucaagugc cgacugaaaa uggacaaacu ucgccugaag ggcgugagcu     1500

acucauugug uacugcugcc uuuacguuca caaagguacc cgcagagacc cuccauggga     1560

cugucaccgu ggaaguucag uaugccggaa cagauggccc uugcaaagug ccagcucaaa     1620

uggccgucga caugcagacc cugacucccg uggguagauu aaucacagca aauccuguaa     1680

uuacggagag uaccgaaaac uccaagauga ugcuggagcu agacccaccc uuuggggaua     1740

gcuacauagu gaucggcguu ggagacaaga aaauuacuca ccacuggcau aggucuggcu     1800

caacaaucgg gaaggcguuc gaagcuaccg uccggggagc caaacgcaug gcagugcucg     1860

gugauacagc cugggacuuc ggcagugugg guggagucuu uaacagccug ggcaagggua     1920

uacaccagau uuucggggcu gccuuuaaau cccuuuucgg cggaaugucu ugguuuucgc     1980

aaaucuugau cggcacucug cucguuuggc uggggcuuaa uaccaagaac ggaagcauuu     2040

cccugacgug uuuggcacuc ggcgguguga ugaucuuccu gucaaccgcu gugagugccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6230
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 2 opt

<400>  6230
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauu aagguggaga      480

acuggaucuu uagaaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauuag gugcaucggc guauccaacc gggacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcacggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgcagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaagaacu uugguggaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauccagcc cgagaaucuc gaauaucgga     1020

uuaugcuguc ggugcacggg ucucaacauu ccggaaugau cgguuacgag accgacgaaa     1080

accgugcgaa ggucgaggug acaccuaauu caccacgcgc cgaggcaacu cuuggcgggu     1140

ucggaagucu gggcuuggau ugugaaccca gaaccggucu cgacuuuagc gaucuguauu     1200

acuuaacaau gaacaauaaa cacuggcugg uacauaagga gugguuccac gacaucccuc     1260

ucccauggca cgcuggggcc gauacuggca ccccgcauug gaacaauaaa gaagcucuug     1320

uugaguuuaa ggacgcccac gcaaaaaggc agacaguggu cgugcuggga ucccaggaag     1380

gcgccgucca uaccgcuuug gcgggggcac uagaggccga gauggaugga gcuaagggua     1440

agcuguucuc uggccaccuc aaaugccggc ugaagaugga caaacuucga cugaaggggg     1500

ugagcuauuc auuguguacu gccgcauuua cguucacaaa aguucccgcc gaaacccucc     1560

auggaacugu gaccguagag guccaguacg cuggcacaga ugguccuugc aagauuccag     1620

ugcaaauggc cguugacaug cagacccuga cucccguggg gcgcuuaaua acagcaaacc     1680

cugucaucac ggaaaguacc gaaaauucca agaugaugcu ggaacuagac ccacccuuug     1740

gcgauagcua cauugugauc ggaguaggcg acaaaaagau aacucaccac uggcauagau     1800

cugggucaac aauuggaaaa gcguucgagg cuaccgugag gggugccaag cggauggcag     1860

uucucggcga uacagccugg gacuucggga gugucggagg cguguuuaac agccugggua     1920

aagggaucca ccagaucuuc ggcgcugccu uuaagucccu uuucggaggc augucuuggu     1980

uuucgcaaau uuugaucggg acucugcucg uguggcuggg acuuaacacc aagaauggca     2040

gcauuucccu gacguguuug gcacucggug gggucaugau auuccuguca accgcuguua     2100

gugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6231
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 2 opt

<400>  6231
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uagggggauu caccagaucu ucggcgccgc guuuaagucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaaaaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6232
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 2 opt

<400>  6232
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc gggcuggcuu uggcagccgc ugccauugcg uggcuccugg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acucuuaauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuucgu agagggcaug uccgggggaa      660

cuuggguuga ugugguccug gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcuu guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuacuu ggacaagcag agcgacaccc aguaugugug caaaaggacu cuggucgauc      900

ggggaugggg caaugguugu gggcuauuug gaaagggcag ucucgugaca ugcgcgaaau      960

ucgccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccug gaguaccgua     1020

uuaugcuuag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucugggcgg guuuggauca uugggccucg acugugagcc uaggaccggu cuggauuuca     1200

gugacuuaua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguuuc     1260

acgauauccc acuuccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu gguagaguuc aaggaugccc acgcuaaacg gcagacaguu guggucuugg     1380

gaagccagga aggcgcggug cauaccgcac uagccggggc ucuggaggcc gaaauggacg     1440

gagcaaaggg ucgacucucc ucuggccacc ugaaaugccg ccuuaagaug gauaagcuga     1500

gauugaaagg ggucagcuau ucacucugua cugccgcuuu uacguucaca aagaucccug     1560

ccgagacccu gcauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagaccuu aacuccugug gggaggcuga     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuagagcucg     1740

aucccccguu uggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauucga agccaccgug cgcggugcua     1860

aaagaauggc cguacugggc gauacagcau gggacuuugg gaguguggga ggcgcucuua     1920

auagcuuggg uaaggggauu caccagaucu ucggcgccgc guucaaaucc cuguuuggag     1980

gcaugucuug guucucgcaa auccucauug ggacucugcu uauguggcug ggauugaaca     2040

ccaagaaugg cagcaucucc cucauguguc uggccuuagg ugggguucug auuuuucuau     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6233
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 2 opt

<400>  6233
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acugguaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caucagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6234
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 2 opt

<400>  6234
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca cccagacucu gacaccugug gggagguuaa     1680

uuacggccaa cccaguuaua accgagagua cugaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacacacc     1800

acuggcaucg gucuggguca accauaggaa aggcauuuga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauacugcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacccuccu gauguggcuu ggacugaaca     2040

cgaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caaccgcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6235
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 2 opt

<400>  6235
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag auccgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6236
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 2 opt

<400>  6236
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacu ggcgcaaaug gcugucgaca ugcagaccuu aacucccgug gggaggcuga     1680

uuacagccaa cccuguuaua acggagagua ccgaaaacuc caagaugaug cuagagcucg     1740

auccacccuu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacugggc gauacagcau gggacuucgg gaguguggga ggcgcucuua     1920

auagcuuggg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc cuguucggag     1980

gcaugucuug guuuucgcaa auccucauug ggacucugcu uauguggcug ggauugaaca     2040

ccaagaaugg cagcaucucc cucauguguc uggccuuagg ugggguucug auuuuccuau     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6237
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 2 opt

<400>  6237
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuacccugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa cacaaccagu acuugggugg ucuauggcac augucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugaccc uaccguccca uucuacgagg aaacuccaaa      420

cucggucgca gaccuggcug gagucacgcg aguacacaaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac uucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

ccuggguuga uguggucuua gaacauggug gcugugugac agugauggcu caggacaagc      720

caaccgucga uauugagcug guuacgacua cagugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acccaagggg      840

aggccuaccu ugacaagcag agcgacacuc aguaugugug caaaaggaca uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugacc ugcgcgaaau      960

uugccugcuc uaagaaaaug acgggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac accggucaug     1080

aaacagauga gaaccgcgca aaggucgaga uuacuccaaa uucccccaga gcugaagcca     1140

cccuuggcgg guucggauca cugggcuugg acugugagcc uaggacaggu cucgauuuua     1200

gugaccugua uuacuuaacu augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacaccgg cacaccccau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagaccguu gugguccugg     1380

gaagccagga gggcgcggug cauacugcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cggccgcuuu cacauuuacc aagaucccug     1560

ccgagacucu ccauggaacc gugacaguug aagugcagua cgcaggcacc gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacucu gacaccugug gggagguuaa     1680

uuacggccaa cccaguuaua accgagagua cugaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacacacc     1800

acuggcaucg gucuggguca accauaggaa aggcauuuga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauacugcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacccuccu gauguggcuu ggacugaaca     2040

cgaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caaccgcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6238
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 2 opt

<400>  6238
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggucgu gagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6239
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 2 opt

<400>  6239
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu gcaugggugg ucuauggcac cugucaucac aaaaagggug      360

aagcccgccg aagccguaga gcugugacuc uaccguccca uucuacaagg aaacuccaaa      420

cccggcccca gacguggcug gagucgcgcg aguacacuaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccu ggguucgcac uggccgcugc cgcgauugca ugguugcucg      540

gaucaagcac cagucagaaa gugaucuauc uggucaugau acugcucauc gccccagcuu      600

acuccauucg gugcaucggc gugucuaauc gcgacuuugu agagggcaug agcgggggaa      660

cauggguuga uguggucuua gaacauggug gcugugugac ugugauggcc caggacaagc      720

ccaccgucga uauugagcug guuacaacca cgguguccaa cauggcagag guacgaucau      780

acugcuauga agcuaguauc agcgacaugg ccucugauuc cagauguccu acucaagggg      840

aggcguaccu ugacaagcag ucagacacac aguaugugug caaaaggacc uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ccuagugacu ugcgccaaau      960

uugcaugcag uaagaaaaug acaggcaagu cuauacagcc agaaaaccuc gaguaccgua     1020

uuaugcuguc cguucacggg agccaacacu cgggaaugau cgugaaugac accggucaug     1080

aaacggauga gaaccgcgcu aaggucgaga uuacccccaa uucuccuaga gccgaagcua     1140

cacuuggcgg guucggaucc cugggcuugg acugugagcc aaggacuggu cucgauuuuu     1200

cagaccugua uuacuuaacc augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc gcuccccugg cacgccgggg cagacacagg cacuccucau uggaacaaua     1320

aagaggcccu uguagaauuu aaggaugcuc acgcgaaacg gcagaccguu gugguccugg     1380

gaagucagga gggcgcagug cauacagccu uggcuggggc ccuagaggca gaaauggacg     1440

gagccaaggg ucgacugagc uccggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaagg ggucucuuau agcuugugua ccgcugccuu cacuuuuacg aagaucccag     1560

cagagacacu ccauggaacc gugacuguug aagugcagua cgcgggcacc gacggucccu     1620

gcaaaguacc ugcucaaaug gccgucgaca ugcagacacu gaccccagug gggagguuaa     1680

uuacugcaaa ccccguuaua acagagucaa cggaaaacag uaagaugaug cuggagcuag     1740

auccgccuuu cggcgacucc uacaucguga uuggagucgg cgagaaaaag aucacccacc     1800

acuggcaucg gagcgggucu acuauaggaa aggccuuuga agcuacagug cgcggugcca     1860

aaagaauggc aguacucggc gauaccgcuu gggacuucgg gucaguggga ggcgcccuga     1920

auagucuugg uaaggggauu caccagaucu ucggcgcggc cuuuaagagc uuguucggag     1980

gcauguccug guuuucucaa auccugauug ggacacuccu gauguggcuu ggacugaaca     2040

cuaagaaugg cucgaucagc uugauguguc ucgcucuggg ugggguuuua auuuuccugu     2100

ccaccgcagu cucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6240
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 2 opt

<400>  6240
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac auccaaaaag gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaggggg      840

aggccuaccu ugacaaacaa agcgacaccc aguaugugug caagaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaaaggcag ucuagugaca ugcgcgaagu      960

uugccugcuc uaaaaagaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcagcacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaagucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua agcacuggcu ggugcauaaa gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aggaggcacu uguagaauuu aaagaugccc acgcuaagcg gcaaacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaagg ucgacugucc ucuggccacc ucaagugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aaaaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaagguacc cgcgcagaug gcugucgaca ugcaaacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caaaaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaagaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aagcauuuga agccaccgug cgcggugcua     1860

agagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaagggauu caccagaucu ucggcgccgc guuuaagucc uuguucggag     1980

gcaugucuug guuuucgcag auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaaaaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6241
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 2 opt

<400>  6241
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac acggcagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acuccauucg cugcaucggc gugucuaauc gagacuuugu agagggcaug agcgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cuguguccaa cauggccgag guaagaucau      780

acugcuauga agcaaguauc agcgacaugg cuucugauuc cagguguccc acacaagggg      840

aggccuaccu ugacaagcag ucagacaccc aguaugugug caaacggacu uuggucgauc      900

guggaugggg caaugguugu gggcuguucg gaaagggcag ccuagugaca ugcgcgaaau      960

uugccugcag uaagaaaaug accggcaagu cuauacagcc ugaaaaccuc gaguaccgca     1020

uuaugcuguc cguucacggg agccaacacu cgggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaacagagca aaggucgaga uuacaccaaa uucucccagg gcugaagcca     1140

cucuuggcgg guucggaucc cugggcuugg acugugagcc ucggaccggu cucgauuuuu     1200

cagaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg acagacaguu gugguccugg     1380

gaagucagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgccugagc uccggccacc ucaaaugcag acugaagaug gauaaacuua     1500

ggcugaaagg ggucucuuau agcuugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggcgguuaa     1680

uuacagccaa cccaguuaua acggagucaa ccgaaaacag uaagaugaug cuggagcuag     1740

aucccccguu cggcgacucc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg cagcgggucu acaauaggaa aggcauuuga agccaccgug agaggugcua     1860

aaaggauggc cguacucggc gauacagcau gggacuucgg gucaguggga ggcgcucuga     1920

auagucuugg uaaggggauu caccagaucu ucggcgccgc guuuaagagc uuguucggag     1980

gcauguccug guuuucucaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cucgaucagc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

ccacggcugu cucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6242
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 2 opt

<400>  6242
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaacccagga gggcgcggug cauacugcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugagc uccggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaagg ggucucuuau agcuugugua cggccgcuuu cacauuuacc aagaucccug     1560

ccgagacucu ccauggaacc gugacaguug aagugcagua cgcaggcacc gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacucu gacaccugug gggagguuaa     1680

uuacggccaa cccaguuaua accgagucaa cugaaaacag uaagaugaug cuggagcuag     1740

aucccccguu cggcgacucc uacaucguga uuggagucgg cgagaaaaag aucacacacc     1800

acuggcaucg gagcgggucu accauaggaa aggcauuuga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauacugcau gggacuucgg gucaguggga ggcgcucuga     1920

auagucuugg uaaggggauu caccagaucu ucggcgccgc guuuaagagc uuguucggag     1980

gcauguccug guuuucucaa auccugauug ggacccuccu gauguggcuu ggacugaaca     2040

cgaagaaugg cucgaucagc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

ccaccgcugu cucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6243
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 2 opt

<400>  6243
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuuuacg gugcauuggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga ugugguccug gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uaucgagcuu guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauu ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuacuu ggacaagcag agcgacaccc aguaugugug caaaaggacu cuggucgauc      900

ggggaugggg caaugguugu gggcuauucg gaaagggcag ucucgugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauccagcc ugaaaaccug gaguaccgua     1020

uaaugcuuag cguucacggg ucgcaacacu cuggaaugau ugugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga ucacaccaaa uucccccaga gcugaagcca     1140

cucugggcgg guucggauca uugggccucg acugugagcc uaggaccggu cuggauuuua     1200

gugacuuaua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc acuuccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu gguagaauuu aaggaugccc acgcuaaacg gcagacaguu guggucuugg     1380

gaagccagga gggcgcggug cauaccgcac uagccggggc ucuggaggcc gaaauggacg     1440

gagcaaaggg ucgacucucc ucuggccacc ugaaaugccg ccuuaagaug gauaagcuga     1500

gauugaaagg ggucagcuau ucacucugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu gcauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagaccuu aacuccugug gggaggcuga     1680

ucacagccaa cccaguuauu acggagagua ccgaaaacuc caagaugaug cuagagcucg     1740

aucccccguu cggcgacagc uacauaguga ucggagucgg cgagaaaaag auuacucacc     1800

acuggcaucg gucuggguca acaaucggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacugggc gauacagcau gggacuucgg gaguguggga ggcgcucuua     1920

auagcuuggg uaaggggaua caccagauuu ucggcgccgc guuuaaaucc cuguucggag     1980

gcaugucuug guuuucgcaa auccucaucg ggacucugcu uauguggcug ggauugaaca     2040

ccaagaaugg cagcauuucc cucauguguc uggccuuagg ugggguucug aucuuccuau     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6244
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 2 opt

<400>  6244
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaaucgaaa      480

auuggauuuu uaggaacccc ggguucgcuc uggcagccgc ugccaucgcg ugguugcucg      540

gaagcaguac aucccagaaa guuauauauc uggugaugau ccugcucauu gcacccgccu      600

acucuauccg gugcauuggc gucagcaauc gcgacuuugu ggagggcaug uccgggggaa      660

cuuggguaga uguuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagccagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagccaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gaguguagga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

caacggcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6245
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 2 opt

<400>  6245
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggauccu gaggaacccc ggguuugcuu uggcagccgc ugccauugcg uggcuccugg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acucuuaauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuucgu agagggcaug uccgggggaa      660

cuuggguuga ugugguccug gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcuu guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuacuu ggacaagcag agcgacaccc aguaugugug caaaaggacu cuggucgauc      900

ggggaugggg caaugguugu gggcuauuug gaaagggcag ucucgugaca ugcgcgaaau      960

ucgccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccug gaguaccgua     1020

uuaugcuuag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucugggcgg guuuggauca uugggccucg acugugagcc uaggaccggu cuggauuuca     1200

gugacuuaua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguuuc     1260

acgauauccc acuuccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu gguagaguuc aaggaugccc acgcuaaacg gcagacaguu guggucuugg     1380

gaagccagga aggcgcggug cauaccgcac uagccggggc ucuggaggcc gaaauggacg     1440

gagcaaaggg ucgacucucc ucuggccacc ugaaaugccg ccuuaagaug gauaagcuga     1500

gauugaaagg ggucagcuau ucacucugua cugccgcuuu uacguucaca aagaucccug     1560

ccgagacccu gcauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagaccuu aacuccugug gggaggcuga     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuagagcucg     1740

aucccccguu uggcgacagc uacaucguga uuggaaucgg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauucga agccaccguc cgcggugcua     1860

aaagaauggc cgugcugggc gauacagcau gggacuuugg gaguguagga ggcgcucuua     1920

auagcuuggg uaaggggauc caccagaucu ucggcgccgc guucaaaucc cuguuuggag     1980

gcaugucuug guucucgcaa auucucaucg ggacucugcu uauguggcug ggauugaaca     2040

ccaagaaugg cagcauuucc cucauguguc uggccuuagg uggggugcug auauuucuau     2100

caacggcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6246
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 2 opt

<400>  6246
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuucaacca cggugaguaa cauggccgag guacgaagcu      780

acugcuauga agcaacuauc ucugacauag cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cguucacggg ucgcaacacu cuggaaugau ugugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacagcg guugugcugg     1380

gaagccagga gggcgcaguc cauaccgccu uggcuggggc ccuagaggca gaaauggacg     1440

gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugcugccuu cacguuuaca aagauuccug     1560

cagagacccu ccauggaacu gucaccgugg aaguucagua cgcgggcaca gacgguccau     1620

gcaaagugcc cgcucaaaug gccguagaca ugcagacccu gacuccuguc gggagguuaa     1680

uaacagcaaa cccagugauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguua ucggaguggg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggccuuuga agcuaccguc cgcggugcca     1860

aaagaauggc agugcucggc gauacagcuu gggacuucgg gaguguagga ggcgcccuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgcggc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguguua auauuccugu     2100

caacggcagu uagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6247
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 2 opt

<400>  6247
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aacugcagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagaccuu aacuccuguc gggaggcuga     1680

uuacagccaa cccagugaua acggagagua ccgaaaacuc caagaugaug cuagagcucg     1740

aucccccguu cggcgacagc uacaucguua uuggaguggg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcugggc gauacagcau gggacuucgg gaguguagga ggcgcucuua     1920

auagcuuggg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc cuguucggag     1980

gcaugucuug guuuucgcaa auccucauug ggacucugcu uauguggcug ggauugaaca     2040

ccaagaaugg cagcaucucc cucauguguc uggccuuagg uggggugcug auuuuccuau     2100

caacggcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6248
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 2 opt

<400>  6248
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ucgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguga ucggagucgg cgagaaaaag auaacccacc     1800

acuggcaucg gucuggguca acuauuggaa aggcauuuga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauaccgcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacacuccu gauguggcuu ggacugaaca     2040

cuaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguuuua auauuccugu     2100

caaccgcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6249
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 2 opt

<400>  6249
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagucaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaguuaa     1500

gacugaaagg ggugagcuau ucauugugua cugcagccuu cacguuuaca aagaucccug     1560

cugagacccu ccauggaacu guuaccgugg aaguacagua cgccggcaca gacgguccau     1620

gcaaaguccc cgcacaaaug gcgguggaca ugcagacccu gacuccuguu gggagguuaa     1680

uuacagcuaa cccagugaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguca uuggaguggg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggccuuuga agcaaccgua cgcggugcca     1860

aaagaauggc ugugcucggc gauacagccu gggacuucgg gaguguugga ggcgcacuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgcugc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uucauguguu uggcgcucgg ugggguccug auuuuucuuu     2100

caacggccgu gagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6250
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 2 opt

<400>  6250
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg uaaacugucc ucuggccacc ucaagugccg acugaagaug gauaaacuuc     1500

gccugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aaaaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaagguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagauuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caaaaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaagaag aucacucacc     1800

acuggcauag gucuggguca acaauaggaa aagcauuuga agccaccgug cggggugcua     1860

agcgcauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaagggauu caccagaucu ucggcgccgc guuuaagucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaaaaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6251
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 2 opt

<400>  6251
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga aggaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaacaaa ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa uaccacaagu accugggugg ucuauggcac uugucaucac aagaaaggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aagcuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa acaccuuauu agaguugaaa      480

acuggaucuu uaggaauccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaag gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaacc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa uauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaaacag agcgacaccc aguaugugug caagaggacu uuggucgauc      900

ggggaugggg caacgguugu gggcuguucg gaaaaggcag ucuagugaca ugcgcgaagu      960

uugccugcuc uaaaaagaug accggcaagu ccauacagcc ugaaaaucuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaacgac acgggucaug     1080

aaaccgauga gaaucgcgca aaagucgaga uuacaccaaa cucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaauaaca agcacuggcu ggugcauaaa gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaauaaca     1320

aggaggcacu uguagaauuu aaagaugccc acgcuaagcg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaagg ucgacugucc ucuggccacc ucaagugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aaaaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaagguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa uccaguuaua acggagagua ccgaaaacuc caaaaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaagaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aagcauuuga agccaccgug cgcggugcua     1860

agagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

acagccuugg uaaagggauu caccagaucu ucggcgccgc guuuaagucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaua     2040

ccaaaaacgg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6252
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 2 opt

<400>  6252
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaggccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaagcc gagauggaug     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gacaaacuua     1500

gacugaaagg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgaaacccu ccauggaacu gugaccguug aggugcagua cgcaggcaca gaugguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggaaagua ccgagaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgaaaagaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga ggccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gacacagcau gggauuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6253
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 2 opt

<400>  6253
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagccg     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggacuggu cucgauuuua     1200

gugaccugua uuacuuaacc augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgccgggg cagacacagg cacuccccau uggaacaaua     1320

aagaggcccu uguagaauuu aaggaugcuc acgcgaaacg gcagaccguu gugguccugg     1380

gaagccagga gggcgcagug cauacagccu uggcuggggc ccuagaggca gaaauggacg     1440

gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua ccgcugccuu cacuuuuacg aagaucccug     1560

cagagacacu ccauggaacc gugacuguug aagugcagua cgcgggcacc gacgguccau     1620

gcaaaguacc cgcucaaaug gccgucgaca ugcagacacu gaccccugug gggagguuaa     1680

uuacugcaaa cccaguuaua acagagagua cggaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacccacc     1800

acuggcaucg gucuggguca acuauaggaa aggccuuuga agcuacagug cgcggugcca     1860

aaagaauggc aguacucggc gauaccgcuu gggacuucgg gaguguggga ggcgcccuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgcggc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa acacugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguuuua aucuuccugu     2100

caacggcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6254
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 2 opt

<400>  6254
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcgcg gacgguccau     1620

gcaaaguacc cgcucaaaug gccgucgaca ugcagacacu gaccccugug gggagguuaa     1680

uuacugcaaa cccaguuaua acagagagua cggaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacccacc     1800

acuggcaucg gucuggguca acuauaggaa aggccuuuga agcuacagug cgcggugcca     1860

aaagaauggc aguacucggc gauaccgcuu gggacuucgg gaguguggga ggcgcccuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgcggc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacacuccu gauguggcuu ggacugaaca     2040

cuaagaaugg cagcaucucc uugauguguc ucgcucuggg ugggguuuua auuuuccugu     2100

caaccgcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6255
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 2 opt

<400>  6255
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcauuagccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga ugugguccug gaacauggug gcugugugac cgugauggcu caggacaagc      720

cuacagucga uauugagcuu guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagaugucca acacaagggg      840

aggccuacuu ggacaagcag agcgacaccc aguaugugug caaaaggacu cuggucgauc      900

ggggaugggg caaugguugu gggcuauucg gaaagggcag ucucgugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc cgaaaaccug gaguaccgua     1020

uuaugcuuag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccuaa uuccccaaga gcugaagcca     1140

cucugggcgg guucggauca uugggccucg acugugagcc caggaccggu cuggauuuua     1200

gugacuuaua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauccc ucuuccaugg cacgcugggg ccgacacugg caccccgcau uggaacaaua     1320

aagaggcacu gguagaauuu aaggaugccc acgcuaaacg gcagacaguu guggucuugg     1380

gaagccagga gggcgcggug cauaccgcac uagccggggc ucuggaggcc gaaauggacg     1440

gagcaaaggg ucgacucucc ucuggccacc ugaaaugccg ccuuaagaug gauaagcuga     1500

gauugaaagg ggucagcuau ucacucugua cugccgcuuu cacguuuaca aagauccccg     1560

ccgagacccu gcauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccuu     1620

gcaaaguacc agcgcaaaug gcugucgaca ugcagaccuu aacucccgug gggaggcuga     1680

uuacagccaa cccuguuaua acggagagua ccgaaaacuc caagaugaug cuagagcucg     1740

auccacccuu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacugggc gauacagcau gggacuucgg gaguguggga ggcgcucuua     1920

auagcuuggg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc cuguucggag     1980

gcaugucuug guuuucgcaa auccucauug ggacucugcu uauguggcug ggauugaaca     2040

ccaagaaugg cagcaucucc cucauguguc uggccuuagg ugggguucug auuuuccuau     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6256
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 2 opt

<400>  6256
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg uguccgauuc aagauguccc acacaagggg      840

aggcuuaccu ugacaagcag agcgacaccc aguaugucug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuaguuaca ugcgccaaau      960

uugcgugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cgugcacggg ucgcaacacu cuggaaugau cgucaaugac acgggucaug     1080

aaaccgauga gaaccgcgcc aagguggaga uuacaccaaa uucccccaga gcagaagcua     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu gguacauaag gaaugguucc     1260

acgauauccc acucccgugg cacgccgggg cugacacugg caccccucau uggaacaaua     1320

aagaggcccu uguugaauuu aaggaugcac acgccaaacg gcagacagug gucgugcugg     1380

gaagccagga gggcgcuguc cauaccgcgu uggcaggggc ccuagaggcu gaaauggacg     1440

gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugagcuau ucauugugua cugcagccuu cacguuuaca aagaucccug     1560

cugagacccu ccauggaacu guuaccgugg aaguacagua cgccggcaca gacgguccau     1620

gcaaaguccc cgcacaaaug gcgguggaca ugcagacccu gacuccuguu gggagguuaa     1680

uuacagcuaa cccagugaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguca uuggaguggg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggccuuuga agcaaccgua cgcggugcca     1860

aaagaauggc ugugcucggc gauacagccu gggacuucgg gaguguugga ggcgcacuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgcugc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uugauguguc ucgcgcuggg uggggucuua auuuuccugu     2100

caacggccgu gagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6257
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 2 opt

<400>  6257
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc auuagcaauc gcgacuuugu ggagggcaug uccgggggaa      660

cuuggguaga uguuguguua gaacauggug gcugugucac cgugauggcu caggacaagc      720

caacagugga uaucgagcug gucaccacga cuguuucaaa cauggccgag gugcgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cgugcacggg ucgcaacacu cuggaaugau uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aagguggaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggaauc cugggcuugg acugugagcc uaggaccggu cucgauuuuu     1200

cagaccugua uuacuuaaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauucc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguggaauuu aaggaugccc acgcuaaacg gcagacagua guugugcugg     1380

gaagucagga gggcgcgguc cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugagc uccggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaagg ggugucuuau agcuugugua cugccgcuuu cacguuuaca aagauaccug     1560

ccgagacccu ccauggaacu gucaccgugg aaguucagua cgcaggcaca gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacccu gacuccuguc gggagguuaa     1680

ucacagccaa cccagugauu acggagucaa ccgaaaacag uaagaugaug cuggagcuag     1740

aucccccguu cggcgacucc uacaucguua uaggaguggg cgagaaaaag auuacucacc     1800

acuggcaucg gagcgggucu acaaucggaa aggcauuuga agccaccguc cgcggugcua     1860

aaagaauggc cgugcucggc gauacagcau gggacuucgg gucaguagga ggcgcucuga     1920

auagucuugg uaaggggauc caccagauuu ucggcgccgc guuuaagagc uuguucggag     1980

gcauguccug guuuucucaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cucgaucagc uugauguguc ucgcccuggg ugggguguua auauuccugu     2100

ccacggcugu uucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6258
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 2 opt

<400>  6258
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac acuugugaug      240

ccaccauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa cacaaccagu acuugggugg ucuauggcac augucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugaccc uaccguccca uucuauuagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauc agaguugaaa      480

auuggauuuu uaggaacccc ggguucgcuc uggcagccgc ugccaucgcg ugguugcucg      540

gaagcaguac aucccagaaa gugauauauc uggucaugau ccugcucauu gcacccgccu      600

acucuauccg gugcauuggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uaucgagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcaua ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauucagcc ugaaaaccuc gaguaccgua     1020

ucaugcugag cguucacggg ucgcaacacu cuggaaugau ugugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagauuccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uaacagccaa cccaguuauc acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauuguga ucggagucgg cgagaaaaag auaacucacc     1800

acuggcaucg gucuggguca acaauuggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauc caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auucugaucg ggacucuccu gauguggcuu ggacugaacg     2040

ccaagaaugg cagcauuucc uugauguguc ucgcucuggg ugggguuuua auauuccugu     2100

caaccgcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6259
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 2 opt

<400>  6259
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacaacg cagccgacgu gccgugacac uaccguccca uucuaccaga aaacuccaga      420

cgaggucgca gacuuggcug gagucacggg aguacaccaa gcaccuuauu cgcguugaaa      480

auuggaucuu uagaaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauuag gugcaucggc gugagcaauc gggacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caagacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgcaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc acgauguccc acacaggggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaagaacu uuggucgaua      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacaacc ugaaaaccuc gaguaccgga     1020

uuaugcugag cguucacggg ucgcagcacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgugca aaggucgaga uuacaccaaa uuccccucgc gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uagaaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg cacaccccau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaaag gcagaccguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucggcugucc ucuggccacc ucaaaugccg acugaagaug gauaaacuuc     1500

gccugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcaaua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcagaug gcugucgaca ugcagacccu gacuccugug gggagauuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcauag gucuggguca acaauaggaa aggcauuuga agccaccgug cggggugcua     1860

aacgcauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccaaaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcag auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6260
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 2 opt

<400>  6260
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaag      420

cucggucgca gacguggcug gagucacgcg aguacacuaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcac uggccgcugc cgcgauugca ugguugcucg      540

gaagcaguac cucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcuu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cauggguuga uguggucuua gaacauggug gcugugugac ugugauggcc caggacaagc      720

caaccgucga uauugagcug guuacaacca cggugucaaa cauggcagag guacgaaguu      780

acugcuauga agcuagcauc ucugacaugg ccuccgauuc aagauguccc acucaagggg      840

aggcguaccu ugacaagcag agcgacacac aguaugugug caaaaggacc uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugacu ugcgccaaau      960

uugcaugcuc uaagaaaaug acaggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac accggucaug     1080

aaacggauga gaaccgcgcu aaggucgaga uuaccccaaa uucccccaga gccgaagcua     1140

cacuuggcgg guucggauca cugggcuugg acugugagcc uaggacuggu cucgauuuua     1200

gugaccugua uuacuuaacc augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgccgggg cagacacagg cacuccccau uggaacaaua     1320

aagaggcccu uguagaauuu aaggaugcuc acgcgaaacg gcagaccguu gugguccugg     1380

gaagccagga gggcgcagug cauacagccu uggcuggggc ccuagaggca gaaauggacg     1440

gagccaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua ccgcugccuu cacuuuuacg aagaucccug     1560

cagagacacu ccauggaacc gugacuguug aagugcagua cgcgggcacc gacgguccau     1620

gcaaaguacc cgcucaaaug gccgucgaca ugcagacacu gaccccugug gggagguuaa     1680

uuacugcaaa cccaguuaua acagagagua cggaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacccacc     1800

acuggcaucg gucuggguca acuauaggaa aggccuuuga agcuacagug cgcggugcca     1860

aaagaauggc aguacucggc gauaccgcuu gggacuucgg gaguguggga ggcgcccuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgcggc cuuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacacuccu gauguggcuu ggacugaaca     2040

cuaagaaugg cagcaucucc uugauguguc ucgcucuggg ugggguuuua auuuuccugu     2100

caaccgcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6261
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 2 opt

<400>  6261
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg ugaagcuauu ucauucccca      180

ccacauuggg caugaacaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuaugag uuacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aucccguaga gccgugacac uaccgucuca uucgaccagg aaacuccaaa      420

cgcggucaca gacuuggcug gagagccgcg aguacaccaa gcaccuuauu agaguugaaa      480

acuggaucuu uaggaauccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaaguuccac aucucagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acagcauucg gugcaucggc guguccaacc gcgacuuugu agagggcaug ucagggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugaguaa uauggccgag guacgaagcu      780

acugcuauga agcaucuauc uccgacaugg cuucagauag cagauguccc acacaagggg      840

aggccuaccu ugacaagcag agugacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgcgaaau      960

uugccugcuc caagaaaaug accggcaaga gcauacagcc ugaaaaucuc gaguaccgua     1020

uuaugcuguc gguucacggg ucucaacacu ccggaaugau cgugaacgac acgggucaug     1080

aaaccgauga gaaucgcgca aaggucgaga uuacaccaaa cucacccaga gcugaagcca     1140

cucuuggcgg guucggaagu cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gcgaccugua uuacuuaaca augaauaaca aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaauaaca     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaucccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaagg ggucucauau aguuugugua cugccgcuuu cacguuuucc aagaucccug     1560

ccgagacacu ccauggaacc gugacugaag agguucagua cgcaggcacc gacgguccau     1620

gcaaagugcc cgcgcaaaug gcuguagaca ugcagacacu gaccccuguc gggagguuaa     1680

uuacugccaa uccagugaua acagaaagca cggagaacuc uaagaugaug cuggagcuag     1740

aucccccguu cggcgacuca uacaucguua uuggaguggg cgaaaagaag aucacccacc     1800

acuggcaucg gagugggagc acuauaggaa aggcauuuga ggccacaguc cgcggugcua     1860

aaagaauggc cgugcucggc gauaccgcau gggacuucgg guccguagga ggcgcucuga     1920

acucucuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucg uuguucggag     1980

gcaugagcug guuuucccaa auccugauug ggacacuccu gauguggcuu ggacugaaua     2040

cuaagaacgg cucaaucagu uugauguguc ucgcccuggg ugggguguua auuuuccugu     2100

cuaccgcugu uagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6262
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 2 opt

<400>  6262
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg ugaagcuauu ucauucccca      180

ccacauuggg caugaacaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuaugag uuacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aucccguaga gccgugacac uaccgucuca uucgaccagg aaacuccaaa      420

cgcggucaca gacuuggcug gagagccgcg aguacaccaa gcaccuuauu agaguugaaa      480

acuggaucuu uaggaauccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaaguuccac aucucagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acagcauucg gugcaucggc guguccaacc gcgacuuugu agagggcaug ucagggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugaguaa uauggccgag guacgaagcu      780

acugcuauga agcaucuauc uccgacaugg cuucagauag cagauguccc acacaagggg      840

aggccuaccu ugacaagcag agugacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgcgaaau      960

uugccugcuc caagaaaaug accggcaaga gcauacagcc ugaaaaucuc gaguaccgua     1020

uuaugcuguc gguucacggg ucucaacacu ccggaaugau cgugaacgac acgggucaug     1080

aaaccgauga gaaucgcgca aaggucgaga uuacaccaaa cucacccaga gcugaagcca     1140

cucuuggcgg guucggaagu cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gcgaccugua uuacuuaaca augaauaaca aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacacugggg cugacaccgg cacaccccau uggaauaaca     1320

aagaggcccu uguagaauuu aaggaugcac acgccaaacg gcagaccguu gugguccugg     1380

gaucccagga gggcgcugug cauacugcgu uggcaggggc ccuagaggcu gaaauggacg     1440

gagccaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaagcuga     1500

gacugaaagg ggucucauau aguuugugua cggcagccuu cacauuuacc aagaucccug     1560

cugagacucu ccauggaacc gugacaguug aagugcagua cgccggcacc gacgguccau     1620

gcaaaguacc cgcacaaaug gcggucgaca ugcagacucu gacaccugug gggagguuaa     1680

uuacggcuaa uccaguuaua accgagggca cugaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggagacagc uacaucguga uuggcgucgg ggagaaaaag aucacacacc     1800

acuggcaucg gucuggauca accauaggua aggccuuuga agcaacagug cgcggcgcca     1860

aaagaauggc uguacucggg gauacugccu gggacuucgg aagugugggc gguguucuga     1920

acagccuugg gaagggcauu caccagaucu ucggagcagc uuuuaaaucc uuguucggcg     1980

ggaugucuug guuuucgcaa auccugauug gaacccuccu gauguggcuu ggccugaaua     2040

cgaagaacgg uagcaucucc uugauguguc ucgcccuugg gggagucuua auuuuccugu     2100

caaccgcggu gagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6263
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 2 opt

<400>  6263
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg ugaagcuauu ucauucccca      180

ccacauuggg caugaacaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuaugag uuacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aucccguaga gccgugacac uaccgucuca uucgaccagg aaacuccaaa      420

cgcggucaca gacuuggcug gagagccgcg aguacaccaa gcaccuuauu agaguugaaa      480

acuggaucuu uaggaauccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaaguuccac aucucagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acagcauucg gugcaucggc guguccaacc gcgacuuugu agagggcaug ucagggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugaguaa uauggccgag guacgaagcu      780

acugcuauga agcaucuauc uccgacaugg cuucagauag cagauguccc acacaagggg      840

aggccuaccu ugacaagcag agugacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgcgaaau      960

uugccugcuc caagaaaaug accggcaaga gcauacagcc ugaaaaucuc gaguaccgua     1020

uuaugcuguc gguucacggg ucucaacacu ccggaaugau cgugaacgac acgggucaug     1080

aaaccgauga gaaucgcgca aaggucgaga uuacaccaaa cucacccaga gcugaagcca     1140

cucuuggcgg guucggaagu cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gcgaccugua uuacuuaaca augaauaaca aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaauaaca     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaucccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucucauau aguuugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa uccaguuaua acggagucca ccgaaaacag caagaugaug cuggagcuag     1740

aucccccguu cggcgacucu uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucagggagu acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gagcguggga ggcgcucuga     1920

acucccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucu uuguucggag     1980

gcaugucgug guuuagccaa auccugauug ggacucuccu gauguggcuu ggacugaaua     2040

ccaagaacgg cuccaucuca uugauguguc ucgcccuggg ugggguuuua auuuuccuga     2100

guacggcugu cucugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6264
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 2 opt

<400>  6264
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc uguugaggug acgagaaggg      120

gaaacgcaua cuauauguac cuggaccggu ccgaugccgg ugaagcgauu ucuuucccca      180

ccacaauggg caugaauaag ugcuauauac agaucaugga cuuggggcac augugugaug      240

cuacuauguc auacgagugc ccuauguuag acgaaggagu agagccagau gacguggauu      300

guuggugcaa caccacaagu accugggucg uguauggcac uugucaucac aaaaagggug      360

aagcccgccg aagccguaga gcaguuacac ugccguccca uucuaccagg aaacuacaaa      420

cgcggucgca gacuuggcuc gagucacgcg aguacaccaa gcaccugauu agaguggaaa      480

auuggaucuu uaggaacccc ggguucgccc uugcugcagc cgcuauugcc uggcuguugg      540

gaagcaguac aucccagaaa gucaucuauc ucgugaugau acugcugauc gcgccugcau      600

acucuauucg gugcaucggc guaagcaauc gcgacuuugu ugagggcaug uccgggggaa      660

cuugggugga ugucgugcuc gaacauggug gcugugugac cgucauggcc caggacaagc      720

caacaguuga uauugaguua gugaccacga cuguaucaaa cauggcugag gugcgaaguu      780

acugcuauga agccagcauc ucugacaugg cauccgauuc aagauguccc acacaagggg      840

aggcuuaccu ggacaagcag agcgacaccc aguaugucug caaaaggacu cuuguggauc      900

ggggaugggg caaugguugu ggguuguucg gaaagggcag ucugguuaca ugcgccaaau      960

uugcgugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccua gaguaccgua     1020

uuaugcucag cgugcacggg ucgcaacacu cuggaaugau cgucaaugac acgggucaug     1080

aaaccgauga gaaccgcgcc aagguggaga uuacaccaaa uucccccaga gcagaagcua     1140

cucugggcgg guucggauca cuuggccugg acugugagcc uaggaccggu uuggauuuua     1200

gugaccucua uuaccugaca augaacaaua aacacugguu aguacauaag gaaugguucc     1260

acgauauccc acugccgugg cacgccgggg cugacacugg cacaccccau uggaacaaua     1320

aagaggcccu cguugaauuu aaggaugcac acgccaaacg gcagaccgug gucgugcuug     1380

gaagccagga gggcgcuguc cauaccgcgc uggcaggggc cuuggaggcu gaaauggacg     1440

gagccaaggg ucgacuaucc ucuggccacc ugaaaugccg ccucaagaug gauaagcuga     1500

gacuuaaagg ggugagcuau ucacugugua cugcagccuu cacguuuaca aagaucccug     1560

cugagaccuu gcauggaacu guuaccgugg aaguacagua cgccggcaca gacgguccau     1620

gcaaaguccc cgcacaaaug gcgguggaca ugcagacccu cacuccuguu gggaggcuga     1680

uuacagcuaa cccagugaua acggagagua ccgaaaacuc caagaugaug uuagagcugg     1740

aucccccguu cggcgacagc uacaucguca uuggaguggg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggccuuuga agcaaccgua cgcggugcca     1860

aaagaauggc ugugcuaggc gauacagccu gggacuucgg gaguguugga ggcgcacuca     1920

auagccuggg uaaggggauu caccagaucu ucggcgcugc cuuuaaaucc cuuuucggag     1980

gcaugucuug guuuucgcaa aucuugauug ggacucugcu cgucuggcug ggacuuaaca     2040

ccaagaaugg cagcaucucc cugauguguu uggcgcucgg uggggugcug auuuucuuau     2100

caacggccgu gagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6265
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 2 opt

<400>  6265
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg ugaagcuauu ucauucccca      180

ccacauuggg caugaacaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuaugag uuacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aucccguaga gccgugacac uaccgucuca uucgaccagg aaacuccaaa      420

cgcggucaca gacuuggcug gagagccgcg aguacaccaa gcaccuuauu agaguugaaa      480

acuggaucuu uaggaauccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaaguuccac aucucagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acagcauucg gugcaucggc guguccaacc gcgacuuugu agagggcaug ucagggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugaguaa uauggccgag guacgaagcu      780

acugcuauga agcaucuauc uccgacaugg cuucagauag cagauguccc acacaagggg      840

aggccuaccu ugacaagcag agugacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgcgaaau      960

uugccugcuc caagaaaaug accggcaaga gcauacagcc ugaaaaucuc gaguaccgua     1020

uuaugcuguc gguucacggg ucucaacacu ccggaaugau cgugaacgac acgggucaug     1080

aaaccgauga gaaucgcgca aaggucgaga uuacaccaaa cucacccaga gcugaagcca     1140

cucuuggcgg guucggaagu cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gcgaccugua uuacuuaaca augaauaaca aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaauaaca     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaucccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucucauau aguuugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa uccaguuaua acggagucca ccgaaaacag caagaugaug cuggagcuag     1740

aucccccguu cggcgacucu uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucagggagu acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gagcguggga ggcgcucuga     1920

acucccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucu uuguucggag     1980

gcaugucgug guuuagccaa auccugauug ggacucuccu gauguggcuu ggacugaaua     2040

ccaagaacgg cuccaucuca uugguuuguc ucgcccuggg uggggucuua auuuuccuga     2100

guacggcugu gucugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6266
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 2 opt

<400>  6266
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg ugaagcuauu ucauucccca      180

ccacauuggg caugaacaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuaugag uuacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aucccguaga gccgugacac uaccgucuca uucgaccagg aaacuccaaa      420

cgcggucaca gacuuggcug gagagccgcg aguacaccaa gcaccuuauu agaguugaaa      480

acuggaucuu uaggaauccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaaguuccac aucucagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acagcauucg gugcaucggc gugaacaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcaucucc uugguuuguc ucgcccuggg uggggucuua auuuuccugu     2100

caacggcugu gagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6267
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 2 opt

<400>  6267
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc uguugaggug acgagaaggg      120

gauccgcaua cuauauguac cuggaccggu cugaugccgg ugaagcgauu ucauucccca      180

ccacauuggg caugaacaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

cuacuaugag uuacgagugc ccuaugcugg acgaaggagu agagccagau gacguggauu      300

guuggugcaa uaccacaagc accugggucg uguauggcac uugucaucac aaaaagggug      360

aagcccgccg aucccguaga gcaguuacac uaccgucuca uucgaccagg aaacuccaaa      420

cgcggucaca gacuuggcug gagagccgcg aguacaccaa gcaccuuauu agaguggaaa      480

acuggaucuu uaggaauccc ggguucgccc uggcugcagc cgcuauugcc ugguugcucg      540

gaaguuccac aucucagaaa gucaucuauc uggugaugau acugcucauc gcgccugcau      600

acagcauucg gugcaucggc guauccaacc gcgacuuugu ugagggcaug ucagggggaa      660

cuugggugga ugucguguua gaacauggug gcugugugac cgucauggcc caggacaagc      720

cagcuguuga uauugagcug gugacaacca cgguaaguaa uauggccgag gugcgaagcu      780

acugcuauga agcaucuauc uccgacaugg cuucagauag cagauguccc acucaagggg      840

aggccuaccu ugacaagcag agugacacac aguaugucug caaaaggacc uugguggauc      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuacu ugcgcgaaau      960

uugccugcuc caagaaaaug acaggcaaga gcauacagcc ugaaaaucuc gaguaccgua     1020

uuaugcuguc ggugcacggg ucucaacacu ccggaaugau cgucaacgac accggucaug     1080

aaacggauga gaaucgcgca aagguggaga uuaccccaaa cucacccaga gcugaagcca     1140

cacuuggcgg guucggaagu cugggcuugg acugugagcc uaggacuggu cucgauuuua     1200

gcgaccugua uuacuuaacc augaauaaca aacacuggcu gguacauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacagg cacuccccau uggaauaaca     1320

aagaggcacu uguugaauuu aaggaugccc acgcuaaacg gcagaccgug gucgugcugg     1380

gaucccagga gggcgcgguc cauacagcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggugucauau aguuugugua ccgccgcuuu cacuuuuacg aagaucccug     1560

ccgagacacu ccauggaacc guuacugugg aaguacagua cgcaggcacc gacgguccau     1620

gcaaaguccc cgcgcaaaug gcuguggaca ugcagacacu gaccccuguu gggagguuaa     1680

uuacugccaa uccagugaua acagagucca cggaaaacag caagaugaug cuggagcuag     1740

aucccccguu cggcgacucu uacaucguca uuggaguggg cgagaaaaag aucacccacc     1800

acuggcaucg gucagggagu acuauaggaa aggcauuuga agccacagua cgcggugcua     1860

aaagaauggc cgugcucggc gauaccgcau gggacuucgg gagcguugga ggcgcucuga     1920

acucccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucu uuguucggag     1980

gcaugucgug guuuagccaa auccugauug ggacacuccu ggucuggcuu ggacugaaua     2040

cuaagaacgg cuccaucuca uugaccuguc ucgcccuggg ugggguguua auuuuccuga     2100

guacggcugu gucugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6268
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 2 opt

<400>  6268
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc uguugaggug acgagaaggg      120

gauccgcaua cuauauguac cuggaccggu cugaugccgg ugaagcgauu ucauucccca      180

ccacauuggg caugaacaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

cuacuaugag uuacgagugc ccuaugcugg acgaaggagu agagccagau gacguggauu      300

guuggugcaa uaccacaagc accugggucg uguauggcac uugucaucac aaaaagggug      360

aagcccgccg aucccguaga gcaguuacac uaccgucuca uucgaccagg aaacuccaaa      420

cgcggucaca gacuuggcug gagagccgcg aguacaccaa gcaccuuauu agaguggaaa      480

acuggaucuu uaggaauccc ggguucgccc uggcugcagc cgucauugcu ugguugcucg      540

gaaguuccac aucucagaaa gugaucuauc ugguaaugau acugcucauc gccccugcgu      600

acagcauucg gugcaucggc guuuccaacc gcgacuuugu ggagggcaug ucagggggaa      660

cuugggucga ugugguguua gaacauggug gcugugucac cguuauggca caggacaagc      720

caacagugga uauugagcug guaaccacga cugugaguaa uauggccgag guccgaagcu      780

acugcuauga ggcuucuauc uccgacaugg ccucagauag cagauguccc acacaagggg      840

aagcauaccu ugacaagcag agugacaccc aguaugugug caaaaggacu uugguugauc      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgcuaaau      960

uugccugcuc caagaaaaug accggcaaga gcauacagcc ugaaaaucuc gaguaccgua     1020

uuaugcuguc gguccacggg ucucaacacu ccggaaugau cgugaacgac acgggucaug     1080

aaaccgauga gaaucgcgcg aagguagaga uuacaccaaa cucacccaga gccgaagcaa     1140

cucuuggcgg guucggaagu cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gcgaccugua uuacuuaaca augaauaaca aacacuggcu gguucauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg cacaccccau uggaauaaca     1320

aagaggcucu uguggaauuu aaggaugccc acgcaaaacg gcagaccguc gugguccugg     1380

gaucccagga gggcgccgug cauaccgcuu uggcgggggc acuagaggcc gaaauggacg     1440

gagcuaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg gguuucauau aguuugugua cugccgcauu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguag aaguccagua cgcuggcaca gacgguccau     1620

gcaaagugcc cgcccaaaug gcaguugaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagcgaa uccagucaua acggagucca ccgaaaacag caagaugaug cuggagcuag     1740

aucccccguu cggcgacucu uacaucguga uuggaguagg cgagaaaaag aucacucacc     1800

acuggcaucg gucagggagu acaauaggaa aggcuuuuga agccaccgug cgcggugcaa     1860

aaagaauggc cguucucggc gauacagcuu gggacuucgg gagcgucgga ggcgcccuga     1920

acucccuugg uaaggggauu caccagaucu ucggcgcagc uuuuaaaucu uuguucggag     1980

gcaugucgug guuuagccaa auccugauug ggacucuccu gguguggcuu ggacugaaua     2040

ccaagaacgg cuccaucuca uugacguguc ucgcccuggg ugggguguua auuuuccuga     2100

guaccgcggu cucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6269
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 2 opt

<400>  6269
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc uguugaggug acgagaaggg      120

gauccgcaua cuauauguac cuggaccggu cugaugccgg ugaagcgauu ucauucccca      180

ccacauuggg cguaaacaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

cuacuaugag uuacgagugc ccuaugcugg acgaaggagu ggagccagau gacgucgauu      300

guuggugcaa uaccacaagc accugggugg uuuauggcac uugucaucac aaaaagggug      360

aagcccgccg aucccguaga gcagugacac uaccgucuca uucgaccagg aaacuccaaa      420

cgcggucaca gacuuggcug gagagccgcg aguacaccaa gcaccuuauu agagucgaaa      480

acuggaucuu uaggaauccc ggguucgccc uggcugcagc cgcuauugcc ugguugcucg      540

gaaguuccac aucucagaaa gugaucuauc ugguaaugau acugcucauc gcgccugcau      600

acagcauucg gugcaucggc guuuccaacc gcgacuuugu ggagggcaug ucagggggaa      660

cuugggucga ugugguguua gaacauggug gcugugucac cguuauggcc caggacaagc      720

caacagugga uauugagcug guaaccacga cugugaguaa uauggcugag guccgaagcu      780

acugcuauga agccucuauc uccgacaugg caucagauag cagauguccc acacaagggg      840

aggcuuaccu ugacaagcag agugacaccc aguaugugug caaaaggacu uugguugauc      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgccaaau      960

uugcgugcuc caagaaaaug accggcaaga gcauacagcc ugaaaaucuc gaguaccgua     1020

uuaugcuguc gguccacggg ucucaacacu ccggaaugau cgugaacgac acgggucaug     1080

aaaccgauga gaaucgcgcc aagguagaga uuacaccaaa cucacccaga gcagaagcua     1140

cucuuggcgg guucggaagu cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gcgaccugua uuacuuaaca augaauaaca aacacuggcu gguucauaag gaaugguucc     1260

acgauauccc acucccgugg cacgccgggg cugacacugg cacaccccau uggaauaaca     1320

aagaggcccu uguggaauuu aaggaugcac acgccaaacg gcagaccguc gugguccugg     1380

gaucccagga gggcgcugug cauaccgcgu uggcaggggc ccuagaggcu gaaauggacg     1440

gagccaaggg ucgacugucu agcggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg gguuucauau aguuugugua cugcagccuu cacguuuaca aagaucccug     1560

cugagacccu ccauggaacu gugaccguag aaguccagua cgccggcaca gacgguccau     1620

gcaaagugcc cgcacaaaug gcgguugaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagcuaa uccagucaua acggagucca ccgaaaacag caagaugaug cuggagcuag     1740

aucccccguu cggcgacucu uacaucguga uuggaguagg cgauaaaaag aucacucacc     1800

acugguaucg gucagggagu acaauaggaa aggccuuuga ggcaaccgug cgcggugcca     1860

aaagaauggc uguucucggc gacacagccu gggauuucgg gagcgucgga ggcgcacuga     1920

acucccuugg uaaggggauu caucagaucu ucggcgcugc cuuuaaaucu uuguucggag     1980

gcaugucgug guuuagccaa auccugauug ggacucuccu gguguggcuu ggacugaaua     2040

ccaagaacgg cuccaucuca uugacguguc ucgcgcuggg ugggguguua auuuuccuga     2100

guaccgccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6270
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 2 opt

<400>  6270
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc uguugaggug acgagaaggg      120

gauccgcaua cuauauguac cuggaccggu cugaugccgg uaaggcgauu ucauucccca      180

ccacauuggg cguaaacaaa ugcuauauac agaucaugga cuuagggcac augugugaug      240

cuacuaugag uuacgaaugc ccuaugcugg acgagggagu ggaaccagau gacgucgauu      300

guuggugcaa uaccacaagc accugggugg uuuauggcac uugucaucac aagaaaggug      360

aggcccgccg aucccguaga gcagugacac uaccgucuca uucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguacaccaa acaccuuauu agagucgaga      480

acuggaucuu uaggaauccc ggguucgccc uggcugcagc cgcuauugcc ugguugcucg      540

gaaguuccac aucucagaag gugaucuauc ugguaaugau acugcucauc gcgccugcau      600

acagcauucg gugcaucggc guuuccaacc gcgacuuugu ggaaggcaug ucagggggaa      660

cuugggucga ugugguguua gagcauggug gcugugucac cguuauggcc caggacaagc      720

caacagugga uauugaacug guaaccacga cugugaguaa uauggcugag guccgaagcu      780

acugcuauga ggccucuauc uccgacaugg caucagauag cagauguccc acacaagggg      840

aggcuuaccu ugacaaacag agugacaccc aguaugugug caagaggacu uugguugauc      900

ggggaugggg caacgguugu gggcuguucg gaaaaggcuc ucuagugaca ugcgccaagu      960

uugcgugcuc caaaaagaug accggcaaga gcauacagcc ugaaaaucuc gaauaccgua     1020

uuaugcuguc gguccacggg ucucaacacu ccggaaugau cgugaacgac auuggucaug     1080

agacggauga aaaucgcgcc aaaguagaga ucaccccaaa cucacccaga gcagaggcua     1140

cacuuggcgg guucggaagu cugggcuugg acugugaacc uaggacuggu cucgauuuua     1200

gcgaccugua uuacuuaacc augaauaaca agcacuggcu gguucauaaa gagugguucc     1260

acgauauccc acucccgugg cacgccgggg cugacacagg cacuccccau uggaauaaca     1320

aggaagcccu uguggaguuu aaagaugcac acgccaagcg gcagaccguc gugguccugg     1380

gaucccagga aggcgcugug cauacagcgu uggcaggggc ccuagaggcu gagauggacg     1440

gagccaaagg ucgacugucu agcggccacc ucaagugccg ccugaagaug gauaaacuua     1500

gacugaaggg gguuucauau aguuugugua ccgcagccuu cacuuuuacg aaaauuccug     1560

cugaaacacu ccauggaacc gugacuguag agguccagua cgccggcacc gacgguccau     1620

gcaaggugcc cgcacaaaug gcgguugaca ugcagacacu gaccccugug gggagguuaa     1680

uaacugcuaa uccagucauc acagaaucca cggagaacag caaaaugaug cuggaacuag     1740

aucccccguu cggcgacucu uacauuguga ucggaguagg cgauaagaag auaacccacc     1800

acuggcaucg gucagggagu acuauuggaa aagccuuuga ggcaacagug cgcggugcca     1860

agagaauggc uguucucggc gacaccgccu gggauuucgg gagcgucgga ggcgcacuga     1920

acucccuugg uaaagggauc caccagaucu ucggcgcugc cuuuaagucu uuguucggag     1980

gcaugucgug guuuagccaa auucugaucg ggacacuccu gguguggcuu ggacugaaua     2040

cuaaaaacgg cuccauuuca uugaccuguc ucgcgcuggg ugggguguua auauuccuga     2100

guacggccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6271
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 2 opt

<400>  6271
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccuuacaa cugccauggc ugcagagaua acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauc ucauucgcca      180

ccacauuggg cgucaacaaa ugccacguuc agauuaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ggaaccugau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugugacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauc aagguugaga      480

acuggauuuu uagaaauccg ggguucacac ugguggcagu cgccaucgcu ugguugcucg      540

gaaguuccac uucucagaag gugauauacc ugguaaugau ccugcucauu gcccccgcgu      600

auagcaucag gugcauuggc guuuccaacc gggacuuugu ggaaggcaug ucagggggaa      660

ccugggucga ugugguguua gagcacggug gcugugucac aguuauggca caggacaaac      720

cuaccgugga uaucgaacug guaacgacua cagugaguaa uauggccgag guccgcagcu      780

acugcuacga ggcuucuaua uccgacaugg ccucagauag ccgaugucca acccaagggg      840

aagcauaucu ugacaagcag agugacacuc aguacgugug caaaagaaca uugguugaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugacc ugcgcuaaau      960

uuacgugcuc caagaagaug accggcaaaa gcauucagcc cgagaaucuc gaauaucgga     1020

ucaugcuguc gguccacggg ucucaacauu ccggaaugau ugugaacgac acaggucacg     1080

agacugauga aaaucgugcc aagguagagg uuaccccuaa cucaccacgc gcggaggcca     1140

cacuuggcgg guucggaagu cugggcuugg acugugaacc cagaacuggu cucgauuuua     1200

gcgaccugua cuauuuaacc augaauaaca aacauuggcu ggugcacaag gagugguucc     1260

acgauauccc ucucccaugg caugcagggg cugacacagg caccccgcac uggaauaaca     1320

aagaagcccu ugucgaguuu aaggaugcuc augccaaaag gcagacugug gucgugcugg     1380

gaucccagga aggcgcaguu cacacggccu uggcuggggc gcuagaggca gagauggacg     1440

gagccaaggg ucggcuguuc ucuggccauc ucaagugccg acugaaaaug gauaaacuuc     1500

gccugaaggg ggugagcuac ucauugugua cagcugccuu uaccuucacu aagguacccg     1560

cagaaacccu ccacggaaca gucaccgugg agguucagua ugccggcacu gacgguccuu     1620

gcaaagugcc agcucaaaug gccgucgaca ugcagacacu gacgcccgug gggagauuaa     1680

ucaccgcaaa uccuguaauu acugaaagua cagagaacuc caagaugaug cuggaacuag     1740

auccacccuu uggcgacagc uacauaguga ucggaguugg cgauaagaaa auuacccacc     1800

auuggcacag gucuggguca acaaucggaa aggcguucga ggcuacuguc cggggugcca     1860

aacgcauggc agugcucggc gacaccgccu gggauuucgg gaguguggga ggcgucuuua     1920

acagccuggg uaaggggaua caucagauuu ucggcgcugc cuuuaaaucc cuuuucggag     1980

gcaugucuug guuuucgcaa aucuugaucg ggacgcugcu cguuuggcug ggacuuaaua     2040

ccaagaacgg cagcauuucc cugacauguu uggcacucgg uggggugaug aucuuccugu     2100

caacugcugu gagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6272
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 2 opt

<400>  6272
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccaacuuggg cgugaauaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacaaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa cacuaccagc acauggguag uguacggcac cugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacuc uaccaucuca cucgacaagg aagcuccaaa      420

cccggucaca gacguggcug gaaagccgcg aguauacuaa acaucuuauu aagguggaga      480

auuggaucuu uagaaacccg ggguucgcac uggccgcugu ugccauagcg ugguugcucg      540

gaaguuccac cucucagaag gugaucuacc uggucaugau ucugcucauc gcacccgccu      600

auagcauuag gugcaucggc guguccaauc gggacuuugu agaaggcaug ucagggggaa      660

cauggguuga uguggucuua gagcacggug gcugugugac ugugauggcu caggacaaac      720

cuaccgucga uauagaacug guuacaacca cggugaguaa cauggccgag guacgcagcu      780

acugcuacga ggcaucuauu uccgacaugg cuucagauag ccgaugucca acucaagggg      840

aagccuaucu ugacaagcag agugacacac aguacgugug caaaagaacc uuggucgaua      900

ggggaugggg caaugguugu gggcuguucg gaaagggcuc ucuagugacu ugcgcgaaau      960

uuacaugcuc caagaagaug accggcaaaa gcauccagcc cgagaaccuc gaauaucgga     1020

uuaugcuguc gguucacggg ucucaacauu ccggaaugau cgugaaugac aucggucacg     1080

agacggauga aaaccgugcc aaggucgagg ugaccccuaa uucaccacgc gcagaggcua     1140

cacuuggcgg guucggaagu cugggcuugg acugugaacc cagaacuggu cucgauuuua     1200

gcgaccugua cuauuuaacc augaacaaua aacauuggcu gguacacaag gagugguucc     1260

acgauauucc ucucccaugg caugccgggg cugacacagg cacuccgcac uggaacaaua     1320

aagaagcccu uguugaguuu aaggaugcac augccaaaag gcagaccgug gucgugcugg     1380

gaucccagga aggcgcuguc cacacagcgu uggcaggggc ccuagaggcu gagauggacg     1440

gagccaaggg ucggcuguuc ucuggccauc ucaagugccg acugaaaaug gauaaacuuc     1500

gccugaaggg ggugagcuac ucauugugua ccgcagccuu uacuuucacg aagguucccg     1560

cugaaacacu ccacggaacc gugacuguag agguccagua ugccggcacc gacgguccuu     1620

gcaaagugcc agcacaaaug gcgguugaca ugcagacacu gacccccgug gggagauuaa     1680

uaacugcuaa cccugucauc acagaaagua cggagaacuc caagaugaug cuggaacuag     1740

auccacccuu uggcgacagc uacauuguga ucggaguagg cgauaagaaa auaacccacc     1800

auuggcacag gucuggguca acuauuggaa aggccuucga ggcaacagug cggggugcca     1860

aacgcauggc uguucucggc gacaccgccu gggauuucgg gagugucgga ggcguguuua     1920

auagccuggg uaagggggug caucagaucu ucggcgcagc uuuuaaaucc cuuuucggag     1980

gcaugucuug guuuucgcaa aucuugauug ggacacugcu cgucuggcug ggacuuaaca     2040

cuaagaaugg cagcaucucc cugaccuguu uggcccucgg ugggguuaug auuuuccugu     2100

caacggcggu gagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6273
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 2 opt

<400>  6273
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauu aagguggaga      480

acuggaucuu uagaaauccg ggguucgcac ugguugccgu ggcuauagcc ugguugcucg      540

gaaguuccac aucucagaag gucaucuacc uggugaugau ucugcucauc gcgcccgcau      600

auagcauuag gugcaucggc guauccaacc gggacuuugu ugaaggcaug ucagggggaa      660

cuugggugga ugucguguua gagcacggug gcugugugac cgucauggcc caggacaaac      720

cuacaguuga uauagaacug gugaccacga cuguaaguaa uauggcugag gugcgcagcu      780

acugcuacga ggccucuauu uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgucug caaaagaacu uugguggaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuaguuaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauccagcc cgaaaaucuc gaauaucgga     1020

uuaugcuguc ggugcacggg ucucaacauu ccggaaugau cgucaacgac aucggucacg     1080

agaccgauga aaaucgugcg aagguggagg uaacaccuaa cucaccacgc gccgaggcaa     1140

cucuuggcgg guucggaagu cugggcuugg acugugaacc cagaaccggu cucgauuuua     1200

gcgaccugua cuauuuaaca augaauaaca aacauuggcu gguucacaag gagugguucc     1260

acgauauucc ucucccaugg caugcugggg ccgacacugg caccccgcac uggaauaaca     1320

aagaagcucu uguggaguuu aaggaugccc augcaaaaag gcagacaguc gugguccugg     1380

gaucccagga aggcgccgug cacaccgcuu uggcgggggc acuagaggcc gagauggacg     1440

gagcuaaggg ucggcuguuc ucuggccauc ucaagugccg acugaaaaug gauaaacuuc     1500

gccugaaggg gguuagcuac ucauugugua cugccgcauu uacguucaca aaggugcccg     1560

ccgaaacccu ccacggaacu guaaccgucg aggugcagua ugcuggcaca gacgguccuu     1620

gcaaaguucc agcccaaaug gcaguggaca ugcagacccu gacucccguc gggagauuaa     1680

uaacagcgaa uccugugauc acggaaagua ccgagaacuc caagaugaug cuggaacuag     1740

auccacccuu uggcgacagc uacauuguaa ucggaguggg cgauaagaaa auaacucacc     1800

auuggcacag gucuggguca acaauuggaa aggcuuucga ggccaccguu cggggugcaa     1860

aacgcauggc cguccucggc gacacagcuu gggauuucgg gaguguggga ggcguguuua     1920

acagccuggg uaaggggauc caucagaucu ucggcgccgc auuuaaaucc cuuuucggag     1980

gcaugucuug guuuucgcaa auuuugaucg ggacucugcu cgucuggcug ggacuuaaua     2040

ccaagaacgg cagcauuucc cugacguguu uggcucucgg ugggguuaug auauuccugu     2100

caaccgccgu gagugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6274
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 2 opt

<400>  6274
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccaacuuggg cgugaauaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacaaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa cacuaccagc acauggguag uguacggcac cugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugucacuc uaccaucuca cucgacaagg aagcuccaaa      420

cccggucaca gacguggcug gaaagccgcg aguauacuaa acaucuuauu aagguggaga      480

auuggaucuu uagaaacccg ggguucgcac uggccgcugu ugccauagcg ugguugcucg      540

gaaguuccac cucucagaag gugaucuacc uggucaugau ucugcucauc gcacccgccu      600

auagcauuag gugcaucggc guguccaauc gggacuuugu agaaggcaug ucagggggaa      660

cauggguuga uguggucuua gagcacggug gcugugugac ugugauggcu caggacaaac      720

cuaccgucga uauagaacug guuacaacca cggugaguaa cauggccgag guacgcagcu      780

acugcuacga ggcaucuauu uccgacaugg cuucagauag ccgaugucca acucaagggg      840

aagccuaucu ugacaagcag agugacacac aguacgugug caaaagaacc uuggucgaua      900

ggggaugggg caaugguugu gggcuguucg gaaagggcuc ucuagugacu ugcgcgaaau      960

uuacaugcuc caagaagaug accggcaaaa gcauccagcc cgagaaccuc gaauaucgga     1020

uuaugcuguc gguucacggg ucucaacauu ccggaaugau cgugaaugac gagaaccgug     1080

ccaaggucga agugacgccu aauucaccac gcgcagaggc uacccuuggu ggcuucggga     1140

gucugggauu ggauugugag cccagaacag gccucgacuu uagcgaucug uacuauuuaa     1200

cuaugaacaa uaaacacugg cugguacaua aggaaugguu ccacgacauc ccucucccau     1260

ggcacgccgg ugcugauacc gggacaccgc auuggaacaa uaaagaggcc cuuguugaau     1320

uuaaggacgc acacgccaaa aggcagacug uggucgugcu gggcucccag gagggagcug     1380

uccauaccgc guuggcaggc gcccuagaag cugagaugga uggggccaag ggacggcugu     1440

ucucugguca ccucaagugc cgacugaaaa uggacaaacu ucgccugaag ggcgugagcu     1500

acucauugug uacagcagcc uuuaccuuca cuaagguucc cgcugagacg cuccauggga     1560

cagugaccgu agaaguccag uaugccggaa cugauggccc uugcaaagug ccagcacaaa     1620

uggcgguuga caugcagacc cugacacccg uggguagauu aauuaccgcu aacccuguca     1680

uaacugagag uacagaaaac uccaagauga ugcuggagcu agacccaccc uuuggggaua     1740

gcuacaucgu gauuggcgua ggagacaaga aaaucacgca ccacuggcau aggucuggcu     1800

caaccauagg gaaggccuuc gaagcaacug ugcggggagc caaacgcaug gcuguucucg     1860

gugauacagc cugggacuuc ggcagugucg ggggaguguu uaauagccug ggcaagggua     1920

uucaccagau cuucggggca gcuuuuaaau cccuuuucgg cggaaugucu ugguuuucgc     1980

aaaucuugau uggcacccug cucguguggc uggggcuuaa cacaaagaau ggaagcaucu     2040

cccugacuug uuuggcccuc ggcgguguca ugauuuuccu gucaaccgcg guuagugccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6275
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 2 opt

<400>  6275
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccuuacaa cugccauggc ugcagagaua acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauc ucauucgcca      180

ccacauuggg cgucaacaaa ugccacguuc agauuaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ggaaccugau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugugacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauc aagguugaga      480

acuggauuuu uagaaauccg ggguucgcac ugguggccgu cgcuaucgcc ugguugcucg      540

gaaguuccac aucucagaag gugauauacc ugguaaugau ccugcucauu gcgcccgcau      600

auagcaucag gugcauuggc guuuccaacc gggacuuugu ggaaggcaug ucagggggaa      660

cuugggucga ugugguguua gagcacggug gcugugucac cguuauggcc caggacaaac      720

cuacagugga uaucgaacug guaaccacga cugugaguaa uauggcugag guccgcagcu      780

acugcuacga ggccucuaua uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgugug caaaagaacu uugguugaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauucagcc cgaaaaucuc gaauaucgga     1020

ucaugcuguc gguccacggg ucucaacauu ccggaaugau ugugaacgac accggucacg     1080

agacagauga aaaucgugcg aagguagagg uuacuccuaa cucaccacgc gccgaggcaa     1140

cccuuggcgg guucggaagu cugggcuugg acugugaacc cagaacaggu cucgauuuua     1200

gcgaccugua cuauuuaacu augaauaaca aacauuggcu ggugcacaag gagugguucc     1260

acgauauccc ucucccaugg caugcugggg ccgacaccgg cacaccgcac uggaauaaca     1320

aagaagcucu ugucgaguuu aaggaugccc augcaaaaag gcagaccgug gucgugcugg     1380

gaucccagga aggcgccguu cacacugcuu uggcgggggc acuagaggcc gagauggacg     1440

gagcuaaggg ucggcuguuc ucuggccauc ucaagugccg acugaaaaug gauaaacuuc     1500

gccugaaggg ggugagcuac ucauugugua cggccgcauu uacauucacc aagguacccg     1560

ccgaaacucu ccacggaacc gucacagugg agguucagua ugcuggcacc gacgguccuu     1620

gcaaagugcc agcccaaaug gcagucgaca ugcagacucu gacacccgug gggagauuaa     1680

ucacggcgaa uccuguaauu accgaaagua cugagaacuc caagaugaug cuggaacuag     1740

auccacccuu uggcgacagc uacauaguga ucggaguugg cgauaagaaa auuacacacc     1800

auuggcacag gucuggguca accaucggaa aggcuuucga ggccacaguc cggggugcaa     1860

aacgcauggc cgugcucggc gacacugcuu gggauuucgg gaguguggga ggcgucuuua     1920

acagccuggg uaaggggaua caucagauuu ucggcgccgc auuuaaaucc cuuuucggag     1980

gcaugucuug guuuucgcaa aucuugaucg ggacccugcu cguuuggcug ggacuuaaua     2040

cgaagaacgg cagcauuucc cugaccuguu uggcucucgg uggggugaug aucuuccugu     2100

caacagccgu gagugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6276
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 2 opt

<400>  6276
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccuuacaa cugccauggc ugcagagaua acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauc ucauucgcca      180

ccacauuggg cgucaacaaa ugccacguuc agauuaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ggaaccugau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuacggcac uugucaacac aagaaaggug      360

aggcccgccg aucccguaga gcugugacac uaccaucuca uucgaccagg aagcuccaga      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaccuuauc aagguugaga      480

acuggauuuu uagaaauccg ggguucgcac ugguggccgu cgcuaucgcc ugguugcucg      540

gaaguuccac aucucagaag gugauauacc ugguaaugau ccugcucauu gcgcccgcau      600

auagcaucag gugcauuggc guuuccaacc gggacuuugu ggaaggcaug ucagggggaa      660

cuugggucga ugugguguua gagcauggug gcugugucac cguuauggcc caagacaaac      720

cuacagugga uaucgaacug guaaccacga cugugaguaa uauggcugag guccgcagcu      780

acugcuacga ggccucuaua uccgacaugg caucagauag ccgaugucca acacaggggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgugug caaaagaacu uugguugaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauucaacc cgaaaaucuc gaauaucgga     1020

ucaugcuguc gguccacggg ucucagcacu ccggaaugau ugugaacgac aucggucaug     1080

agaccgauga aaaucgugcg aagguagagg uuacaccuaa cucaccacgc gccgaggcaa     1140

cucuuggcgg guucggaagu cugggcuugg acugugaacc cagaaccggu cucgauuuua     1200

gcgaccugua cuauuuaaca augaauaaca aacacuggcu ggugcauaag gagugguucc     1260

acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau uggaauaaca     1320

aagaagcucu ugucgaguuu aaggaugccc acgcaaaaag gcagacagug gucgugcugg     1380

gaucccagga aggcgccguu cauaccgcuu uggcgggggc acuagaggcc gagauggacg     1440

gagcuaaggg ucggcuguuc ucuggccacc ucaagugccg acugaaaaug gauaaacuuc     1500

gccugaaggg ggugagcuac ucauugugua cugccguaug cacggcagcc aagguccccg     1560

cugaaacacu ccauggaacc gugacuguug aggugcaaua ugccggcacc gacgguccuu     1620

guaaaguccc agcacagaug gcgguggaca ugcagacacu gacccccgua gggagauuaa     1680

uuacugcuaa uccugugaua acagaaagua cggagaacuc caagaugaug cuggaacuag     1740

auccacccuu uggcgacagc uacaucguua uuggagucgg cgauaagaaa aucacccacc     1800

acuggcauag gucuggguca acuauaggaa aggccuucga ggcaacagug cggggugcca     1860

aacgcauggc ugugcucggc gacaccgccu gggauuuugg gagugucgga ggcguuuuca     1920

acagccuggg uaaggggauu caccaaaucu ucggcgcagc uuuuaaaucc cuuuucggag     1980

gcaugucuug guuuucgcag aucuugauug ggacacugcu cguguggcug ggacuuaaua     2040

cuaagaacgg cagcaucucc cugaccugcu uggcccucgg uggggugaug auuuuccugu     2100

caacggcggu aagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6277
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 2 opt

<400>  6277
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccuuacaa cugccauggc ugcagagaua acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauc ucauucgcca      180

ccacauuggg cgucaacaaa ugccacguuc agauuaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ggaaccugau gacguagauu      300

guuggugcaa uaccacaagc accugggugg ucuacggcac uugucaccau aagaaaggug      360

aggcccgccg aucccguaga gcugugacac uaccaucuca cucgaccagg aagcuccaaa      420

cgcggucaca gacuuggcug gaaagccgcg aguauaccaa acaucuuauc aagguugaga      480

acuggauuuu uagaaauccg ggguucgcac ugguggccgu cgcuaucgcc ugguugcucg      540

gaaguuccac aucucagaag gugauauacc ugguaaugau ccugcucauu gcgcccgcau      600

auagcaucag gugcauuggc guuuccaacc gggacuuugu ggaaggcaug ucagggggaa      660

cuugggucga ugugguguua gagcacggug gcugugucac cguuauggcc caggacaaac      720

cuacagugga uaucgaacug guaaccacga cugugaguaa uauggcugag guccgcagcu      780

acugcuacga ggccucuaua uccgacaugg caucagauag ccgaugucca acacaagggg      840

aggcuuaucu ugacaagcag agugacaccc aguacgugug caaaagaacu uugguugaua      900

ggggaugggg caacgguugu gggcuguucg gaaagggcuc ucuagugaca ugcgccaaau      960

uuaccugcuc caagaagaug acgggcaaaa gcauucagcc cgaaaaucuc gaauaucgga     1020

ucaugcuguc gguccacggg ucucaacauu ccggaaugau ugugaacgac gagaaucgug     1080

cgaagguaga aguuaccccu aacucaccac gcgccgaggc aacacuuggu ggcuucggga     1140

gucugggauu ggauugugag cccagaacug gccucgacuu uagcgaucug uacuauuuaa     1200

ccaugaauaa caaacacugg cuggugcaua aggaaugguu ccacgacauc ccucucccau     1260

ggcacgcugg ugccgauaca gggacuccgc auuggaauaa caaagaggcu cuugucgaau     1320

uuaaggacgc ccacgcaaaa aggcagaccg uggucgugcu gggcucccag gagggagccg     1380

uucauacagc uuuggcgggc gcacuagaag ccgagaugga uggggcuaag ggacggcugu     1440

ucucugguca ccucaagugc cgacugaaaa uggacaaacu ucgccugaag ggcgugagcu     1500

acucauugug uaccgccgca uuuacuuuca cgaagguacc cgccgagaca cuccauggga     1560

ccgucacugu ggaaguucag uaugcuggaa ccgauggccc uugcaaagug ccagcccaaa     1620

uggcagucga caugcagaca cugacccccg uggguagauu aaucacugcg aauccuguaa     1680

uuacagagag uacggaaaac uccaagauga ugcuggagcu agacccaccc uuuggggaua     1740

gcuacauagu gaucggcguu ggagacaaga aaauuaccca ccacuggcau aggucuggcu     1800

caacuaucgg gaaggcuuuc gaagccacag uccggggagc aaaacgcaug gccgugcucg     1860

gugauaccgc uugggacuuc ggcagugucg ggggaguguu uaacagccug ggcaagggua     1920

uacaccagau uuucggggcc gcauuuaaau cccuuuucgg cggaaugucu ugguuuucgc     1980

aaaucuugau cggcacacug cucguuuggc uggggcuuaa uacuaagaac ggaagcauuu     2040

cccugaccug uuuggcucuc ggcgguguga ugaucuuccu gucaacggcc gugagugcgu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6278
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 2 opt

<400>  6278
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagagauc acgagaaggg      120

gauccgccua cuauauguac cuggaccggu cugaugcggg uaaggcuauu ucauucgcca      180

ccacauuggg cgugaacaaa ugccacgucc agaucaugga cuuagggcau augugugaug      240

caacuaugag uuaugaaugc cccaugcugg acgagggagu ugaaccugau gacguggauu      300

guuggugcaa uaccacaagc accuggguag uguacggcac uugucaccau aagaaaggug      360

agacacgccg aucccguaga ucugucucgc uaagguauca cuacacccgg aagcuccaaa      420

cgcgcucaca gacuuggcug gaaagcagag aguauaaaaa gcaucuuauu augguggaga      480

acuggaucuu uaggaaucca ggguucgcca uaguuagugu ggcuaucacc uggcugaugg      540

gauccuugac aucucagaag gucauuuacc ucgugaugau cguacugauu guuccggcau      600

acagcaucuc cugcauaggc gugucaaacc gggaccuggu cgaaggcaug agugggggaa      660

cuugggugga ugugguccuc gagcacggug gcuguguuac cgaaauggcc caggacaaac      720

ccacagugga uauugaguua guaaccauga cggugagcaa uauggcugag guccgcucuu      780

auugcuacga agccucccug ucagacaugg cgagcgcaag ucgauguccu acucaagggg      840

agccaucucu ugauaagcag uccgacacac agagcgugug caaaagaacc uugggagaca      900

ggggcugggg uaacgggugu ggaaucuuug gcaagggcuc gcugguuacu ugcucuaaau      960

ucacaugcug uaagaagaug cccgggaaau ccauucagcc ugaaaaucua gaguaucgga     1020

ucaugcuccc agugcacgga ucacaacaua gugguaugau cgucaacgau auuggccacg     1080

aaaccgacga gaaucgugcc aagguggagg uaacgcccaa cagcccucgc gcugaagcca     1140

cccugggagg guuuggcucc cuuggucugg auugcgagcc aagaacaggg uuggacuucu     1200

cugaucucua cuaucugacu augaauaaca aacauugguu aguucacaag gaaugguuuc     1260

acgacauacc gcugcccugg caugcaggcg cugauaccgg aacaccucac uggaauaaca     1320

aagaggcccu cguggaguuc aaggacgcgc augccaaaag gcagacuguc gugguccuug     1380

gcagccagga aggggcagug cacaccgcuc uggccggagc uuuggaggcc gaaauggaug     1440

gugcaaaggg ccggcuauuu ucagggcauc ugaagugucg acucaaaaug gacaagcugc     1500

gccuuaaagg aguuaguuac ucccugugca cagccgcuuu caccuuuacu aaggugccag     1560

cggagacguu gcacggcaca guaaccgucg aagugcagag cgcagguacu gaugggcccu     1620

guaaaguucc ugcccaaaug gcuguggaca ugcagacccu cacaccaguc ggcagacuga     1680

ucaccgccaa ucccgugauu acugagucua cagaaaacuc aaagaugaug uuagagcugg     1740

acccgccuuu cggagauagu uauaucguaa uaggcguggg ggacaagaaa auuacgcacc     1800

auuggcacag gagcggaucc accaucggua aggcauuuga ggccacuguu cggggcgcua     1860

aacgcauggc cguccuaggg gauacagcau gggacuucgg aucugugggc gguguguuca     1920

acucgcucgg gaagggcauc caucagauuu uuggagcggc uuucaaaagc cuguuuggcg     1980

ggauguccug guucucacaa auccuuauug gaaccuugcu ggucuggcuc ggccugaaua     2040

caaagaacgg uaguauaucu cuuacuugcc uggcccucgg gggaguuaug aucuuuuuga     2100

gcaccgcagu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6279
<211>  2446
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 2 opt

<400>  6279
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcg       60

ugggcaucgu cgggcugcuc cuuaccacag ccauggcugc agagguuacu agaaggggau      120

ccgccuacua uauguaccug gaccggaacg augcggguga agcuauuucu uuccccacga      180

ccuugggcau gaauaagugc uauauacaga ucauggacuu agggcacaug ugugaugcca      240

caaugucaua cgagugcccu augcuggacg aaggagugga gccagaugac guagauuguu      300

ggugcaacac uaccaguaca uggguggucu auggcaccug ucaucacaaa aagggugaag      360

cacgccgaag ccguagagcc gugacucuac cgucccauuc uacaaggaaa cuccaaaccc      420

ggucgcagac guggcuggag ucacgcgagu acacuaagca ccuuauuaga guugaaaauu      480

ggaucuuuag gaaccccggg uucgcucugg cagccgcugc cauugcgugg uugcucggaa      540

gcaguaccuc ccagaaagug aucuaucugg ucaugauacu gcucaucgcc ccugcauacu      600

cuauucggug caucggcgug agcaaucgcg acuuuguaga gggcaugucc gggggaacau      660

ggguugaugu ggucuuagaa caugguggcu gugugacugu gauggcucag gacaagccaa      720

ccgucgauau ugagcugguu acaaccacgg ugucaaacau ggccgaggua cgaaguuacu      780

gcuaugaagc aagcaucucu gacauggcuu ccgauucaag augucccacu caaggggagg      840

ccuaccuuga caagcagagc gacacacagu augugugcaa aaggaccuug gucgaucggg      900

gauggggcaa ugguuguggg cuguucggaa agggcagucu agugacuugc gcgaaauuug      960

ccugcucuaa gaaaaugaca ggcaagucca uacagccuga aaaccucgag uaccguauua     1020

ugcugagcgu ucacgggucg caacacucug gaaugaucgu gaaugacacc ggucaugaaa     1080

cggaugagaa ccgcgcaaag gucgagauua ccccaaauuc ccccagagcu gaagccacac     1140

uuggcggguu cggaucacug ggcuuggacu gugagccuag gacuggucuc gauuuuagug     1200

accuguauua cuuaaccaug aacaauaaac acuggcuggu gcauaaggaa ugguuccacg     1260

auaucccacu cccguggcac gcuggggccg acacaggcac uccccauugg aacaauaaag     1320

aggcacuugu agaauuuaag gaugcccacg cuaaacggca gaccguugug guccugggaa     1380

gccaggaggg cgcggugcau acagcauugg ccggggcucu agaggccgaa auggacggag     1440

caaagggucg acuguccucu ggccaccuca aaugccgccu gaagauggau aaacuuagac     1500

ugaagggggu cagcuauuca uuguguaccg ccgcuuucac uuuuacgaag aucccugccg     1560

agacacucca uggaaccgug acuguugaag ugcaguacgc aggcaccgac gguccaugca     1620

aaguacccgc gcaaauggcu gucgacaugc agacacugac cccugugggg agguuaauua     1680

cugccaaccc aguuauaaca gagaguacgg aaaacuccaa gaugaugcug gagcuagauc     1740

ccccguucgg cgacagcuac aucgugauug gagucggcga gaaaaagauc acccaccacu     1800

ggcaucgguc ugggucaacu auaggaaagg cauuugaagc cacagugcgc ggugcuaaaa     1860

gaauggccgu acucggcgau accgcauggg acuucgggag ugugggaggc gcucugaaua     1920

gccuugguaa ggggauucac cagaucuucg gcgccgcguu uaaauccuug uucggaggca     1980

ugucuugguu uucgcaaauc cugauuggga cacuccugau guggcuugga cugaacacua     2040

agaauggcag caucuccuug augugucucg cccugggugg gguuuuaauu uuccugucaa     2100

ccgcugucag ugcaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2160

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2220

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2280

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2400

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2446


<210>  6280
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 2 opt

<400>  6280
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uauugagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga uuacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

uuacagccaa cccaguuaua acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacucacc     1800

acuggcaucg gucuggguca acaauaggaa aggcauuuga agccaccgug cgcggugcua     1860

gaaggauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaagggauu caccagaucu ucggcgccgc guuuaagucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacucuccu gauguggcuu ggacugaaca     2040

ccaaaaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6281
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 2 opt

<400>  6281
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggauguu uaggaacccc ggguucgcuc uggcagccgc ugccaucgcg ugguugcucg      540

gaagcaguac aucccagaaa gugauuuauc uggucaugau ccugcucaua gcacccgccu      600

acucuauccg gugcauuggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgugauggcu caggacaagc      720

caacagucga uaucgagcug guuaccacga cugugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauu ucugacaugg cuuccgauuc aagauguccc acacaagggg      840

aggccuaccu ugacaagcag agcgacaccc aguaugugug caaaaggacu uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugaca ugcgcgaaau      960

uugccugcuc uaagaaaaug accggcaagu ccauccagcc ugaaaaccuc gaguaccgua     1020

uaaugcugag cguucacggg ucgcaacacu cuggaaugau ugugaaugac acgggucaug     1080

aaaccgauga gaaccgcgca aaggucgaga ucacaccaaa uucccccaga gcugaagcca     1140

cucuuggcgg guucggauca cugggcuugg acugugagcc uaggaccggu cucgauuuua     1200

gugaccugua uuacuuaaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauucc acucccgugg cacgcugggg ccgacacugg caccccucau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagacaguu gugguccugg     1380

gaagccagga gggcgcggug cauaccgcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua cugccgcuuu cacguuuaca aagaucccug     1560

ccgagacccu ccauggaacu gugaccguug aagugcagua cgcaggcaca gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacccu gacuccugug gggagguuaa     1680

ucacagccaa cccaguuauu acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauaguga ucggagucgg cgagaaaaag auuacucacc     1800

acuggcaucg gucuggguca acaaucggaa aggcauuuga agccaccgug cgcggugcua     1860

aaagaauggc cguacucggc gauacagcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggaua caccagauuu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugaucg ggacucuccu gauguggcuu ggacugaaca     2040

ccaagaaugg cagcauuucc uugauguguc ucgcccuggg ugggguuuua aucuuccugu     2100

caacggcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6282
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 2 opt

<400>  6282
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caugaccagu acaugggugg ucuauggcac cugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacuc uaccguccca uucuacaagg aaacuccaaa      420

cccggucgca gacguggcug gagucacgcg aguacacuaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac cucccagaaa gugaucuauc uggucaugau acugcucauc gccccugcau      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cauggguuga uguggucuua gaacauggug gcugugugac ugugauggcu caggacaagc      720

caaccgucga uauugagcug guuacaacca cggugucaaa cauggccgag guacgaaguu      780

acugcuauga agcaagcauc ucugacaugg cuuccgauuc aagauguccc acucaagggg      840

aggccuaccu ugacaagcag agcgacacac aguaugugug caaaaggacc uuggucgauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagugacu ugcgcgaaau      960

uugccugcuc uaagaaaaug acaggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cguucacggg ucgcaacacu cuggaaugau cgugaaugac accggucaug     1080

aaacggauga gaaccgcgca aaggucgaga uuaccccaaa uucccccaga gcugaagcca     1140

cacuuggcgg guucggauca cugggcuugg acugugagcc uaggacuggu cucgauuuua     1200

gugaccugua uuacuuaacc augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc acucccgugg cacgcugggg ccgacacagg cacuccccau uggaacaaua     1320

aagaggcacu uguagaauuu aaggaugccc acgcuaaacg gcagaccguu gugguccugg     1380

gaagccagga gggcgcggug cauacagcau uggccggggc ucuagaggcc gaaauggacg     1440

gagcaaaggg ucgacugucc ucuggccacc ucaaaugccg ccugaagaug gauaaacuua     1500

gacugaaggg ggucagcuau ucauugugua ccgccgcuuu cacuuuuacg aagaucccug     1560

ccgagacacu ccauggaacc gugacuguug aagugcagua cgcaggcacc gacgguccau     1620

gcaaaguacc cgcgcaaaug gcugucgaca ugcagacacu gaccccugug gggagguuaa     1680

uuacugccaa cccaguuaua acagagagua cggaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacaucguga uuggagucgg cgagaaaaag aucacccacc     1800

acuggcaucg gucuggguca acuauaggaa aggcauuuga agccacagug cgcggugcua     1860

aaagaauggc cguacucggc gauaccgcau gggacuucgg gaguguggga ggcgcucuga     1920

auagccuugg uaaggggauu caccagaucu ucggcgccgc guuuaaaucc uuguucggag     1980

gcaugucuug guuuucgcaa auccugauug ggacacuccu gauguggcuu ggacugaaca     2040

cuaagaaugg cagcaucucc uugauguguc ucgcccuggg ugggguuuua auuuuccugu     2100

caaccgcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6283
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 2 opt

<400>  6283
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcagagguu acgagaaggg      120

gauccgccua cuauauguac cuggaccgga acgaugcggg ugaagcuauu ucuuucccca      180

ccacauuggg caugaauaag ugcuauauac agaucaugga cuuagggcac augugugaug      240

ccacuauguc auacgagugc ccuaugcugg acgaaggagu ggagccagau gacguagauu      300

guuggugcaa caccacaagu accugggugg ucuauggcac uugucaucac aaaaagggug      360

aagcacgccg aagccguaga gccgugacac uaccguccca uucuaccagg aaacuccaaa      420

cgcggucgca gacuuggcug gagucacgcg aguacaccaa gcaccuuauu agaguugaaa      480

auuggaucuu uaggaacccc ggguucgcuc uggcagccgc ugccauugcg ugguugcucg      540

gaagcaguac aucccagaaa gugaucuauc uggucaugau acugcucauc gcacccgccu      600

acucuauucg gugcaucggc gugagcaauc gcgacuuugu agagggcaug uccgggggaa      660

cuuggguuga uguggucuua gaacauggug gcugugugac cgcuauggcc caggacaagc      720

caacagugga uauugagcug gucaccacga cuguuucaaa cauggcagag gugcgaaguu      780

acugcuauga agcuagcauc ucugacaugg ccuccgauuc aagauguccc acacaagggg      840

aggcguaccu ugacaagcag agcgacaccc aguauguaug caaaaggacu uugguggauc      900

ggggaugggg caaugguugu gggcuguucg gaaagggcag ucuagucaca ugcgccaaau      960

uugcaugcuc uaagaaaaug accggcaagu ccauacagcc ugaaaaccuc gaguaccgua     1020

uuaugcugag cgugcacggg ucgcaacacu cuggaaugcu uguuaaugac acgggucaug     1080

aaaccgauga gaaccgcgcu aagguggaga ucacaccaaa uucccccaga gccgaagcua     1140

cucugggcgg guucggauca uugggccucg acugugagcc uaggaccggu cuggauuuua     1200

gugacuuaua uuaccugaca augaacaaua aacacuggcu cgcccauaag gaaugguucc     1260

acgauauucc acuuccgugg cacgcagggg ccgcuacugg caccccucau uggaacaaua     1320

aagaggcgcu ggucgaauuu aaggacgcac acgccaaacg gcagacagug guaguuuugg     1380

gaagccagga gggcgcugug cauaccgccc uagcaggggc ccuggaggcu gaaauggaug     1440

gagccaaggg ucgacucucc ucuggccacc ugaaaugccg ccuuaagaug gacaagcuga     1500

gauugaaagg ggucagcuau ucacucugua cugcagcguu cacguuuaca aagaucccug     1560

cugagaccgu ggauggaacu gucaccgugg aaggccagua cggugggaca gacggcccau     1620

gcaaaguucc cgcccaaaug gcaguggaca ugcagacccu gacuccugua ggaagguuaa     1680

ucacagccaa cccagucauu acggagagua ccgaaaacuc caagaugaug cuggagcuag     1740

aucccccguu cggcgacagc uacauaguga ucgggguugg agagaaaaag auuacucauc     1800

acuggcaccg gucugguuca acaaucggca aggcuuuuga agccaccgug cgcggggcaa     1860

aaagaauggc uguccucgga gauacagccu gggacuucgg cagugugggu ggggcgcuga     1920

auagccuugg caagggaaua caucagauua ucggcgccgc uuucaaaucc uuguuugggg     1980

gaaugucuug guucucgcaa auccugauug gcacucuccu gauguggcuu ggucugaaca     2040

ccaagaaugg gagcaucucc uugauguguc ucgcacuggg aggcguauua auuuuucugu     2100

caacggccgu gagugguuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6284
<211>  2440
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 2 opt

<400>  6284
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacgcuag caucgggauu gugucccugc uccuuaccac agucauggca gccgagauaa      120

cuagaagggg aucugcguac uauauguacc uggaucgguc agacgcuggu aaggccauca      180

guuucguuac gaccuugggc gugaacaaau gccacguaca gauuauggau uuagggcaua      240

ugugugacgc aacaaugagc uaugaaugcc ccaugcugga ugagggagug gaaccugacg      300

augucgacug uuggugcaau acuaccucca cauggguggu uuacggcacc ugucaccaua      360

agaaagguga ggcccgccga ucucguagag cugugacucu accaucgcac ucaacaagga      420

agcuccaaac ccggagccag acguggcugg aaagucgcga guauacuaaa caucuuauca      480

aggucgagaa cuggauuuuu agaaauccgg gguucgcacu gguggccgua gcuaucgccu      540

gguugcucgg auccucuacc agccagaagg uuauauaccu ggugaugauc cugcucauug      600

cgcccgcaua uuccaucagg ugcauuggcg ucucaaaccg ggauuuugug gaaggcauga      660

gugggggaac auggguggac gucguuuuag agcacggugg cugugugacu guaauggccc      720

aggauaaacc uaccguggac aucgaacugg ucacaaccac ggugagcaau auggcugagg      780

uucgcucuua cugcuacgag gccuccauau cagauauggc aagcgacagu cgauguccaa      840

cucaagggga ggcuuaucuu gacaagcagu cugauacaca guacgugugc aaaagaaccu      900

uggucgacag gggauggggc aacgguugug ggcuguucgg aaagggcucc cuagugacuu      960

gcgccaaauu uacaugcagc aagaagauga ccggcaaauc gauucagccc gaaaaucucg     1020

aauaucggau caugcugucu guacacgggu cccaacauuc aggaaugauu guuaacgaug     1080

agaaucgugc gaagguggaa gucacgccua acaguccacg cgccgaggca acccuuggug     1140

gcuucgggag ccugggauug gacugugagc ccagaacagg ccucgauuuu uccgaccugu     1200

acuauuuaac uaugaauaac aaacacuggc uggugcauaa ggaaugguuc cacgauaucc     1260

cucucccaug gcacucuggu gcugacaccg agacaccgca uuggaauaac aaagaagccc     1320

uugucgaguu uaaggaugcu cacgccaaaa ggcagacugu gguugugcug gggagccagg     1380

aaggcgcagu acauaccgcc uuggcuggag cgcuagaggc agagauggac ggcgccaagg     1440

ggcggcuguc aaguggacac cucaagugcc gacugaaaau ggauaaacuu cgccugaagg     1500

gugucuccua cagcuugugu acagcugccu ucaccuuuac uaaggugccc gcagaaacgc     1560

uccauggcac aguuaccgug gagguccagu augccgggag agacggaccu ugcaaagugc     1620

cagcucaaau ggccguagac augcagacuc ugacccccgu gggcagguua aucacagcaa     1680

auccuguuau uaccgaaucu acugagaacu caaagaugau gcuggaacua gauccacccu     1740

ucggugacag uuacauaguc aucggggugg gcgauaagaa aauuacacac cacuggcauc     1800

ggagcggauc caucauaggc aaggcguuug aggcuacggu gcgcggggcc aaaagaaugg     1860

caguccucgg agacaccgcc ugggauuucg guucuguugg cgggguguuc aacucgcugg     1920

gaaagggcau ucaccagauc uuuggugcug ccuucaaaag ccuuuuuggg ggcauguccu     1980

gguucucaca aaucuugauu ggaacucugc ucguguggcu gggccuuaau acaaagaacg     2040

ggaguaucuc ucugaccugu uuggcacucg gaggcguaau gauuuuucug agcacagcug     2100

ucuccgccug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2160

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2220

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2280

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2400

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2440


<210>  6285
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 2 opt

<400>  6285
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag caucgggauu auaggacugc uccuuacaac ugcuauggca gccgagauca      120

cgagaagggg uuccgcguac uauauguacc uggaucgguc ugacgcuggc aaggccauuu      180

cauucgcaac cacaaugggg gugaacaaau gccacgucca gaucauggau uugggacaua      240

ugugugacgc cacuaugagu uaugaaugcc ccauguuaga ugagggcguu gaaccugacg      300

auguggacug uuggugcaau accacaagca ccuggguagu guacgguacu ugucaccaua      360

agaaagggga ggcucgccca ucccgacgug cagucacacu gccgucucac ucgaccagaa      420

agcuacaaac gaggucacag acuuggcucg aaagccggga guauaccaaa caucugauua      480

agguggagaa cuggauccuu cgcaaucccg gauuuacagu ugccgcucug gccauggcgu      540

gguugcucgg caacaguacu ucccagagag ugauauaccu ggucaugauc cugcucauug      600

cacccgccua uucuaucagg ugcauuggcg ugagcaaucg ggauuucgua gaagggaugu      660

ccggagguac cuggguugac guggucuuag agcacggcgg gugugugaca gugauggcuc      720

aggauaagcc aaccgucgac aucgaacugg uuacgacuac agugucaaac auggccgagg      780

uacgcaguua cugcuacgag gcaagcauau cugauauggc uuccgacuca cgauguccca      840

cccaaggaga agccuaucuu gacaaacaga gcgauacuca guacgugugc aagagaacau      900

uggucgacag gggcuggggu aaugggugug gacuguuugg caaaggcagu cuagugaccu      960

gcgcgaaguu cacgugcucu aaaaagauga ccgggaaguc cauucagccu gagaaccucg     1020

aauaucggau caugcugagc guucacggau cgcaacauuc ugguaugauu gugaaugaua     1080

ucggccacga gacagacgaa aaccgugcca aagucgaggu gacuccaaau uccccucgcg     1140

cagaggcuac ccuuggggga uuuggcucac uggguuugga uugugaaccu agaacagggc     1200

ucgacuucag ugaucuguac uauuuaacua ugaacaauaa gcauuggcug guacacaaag     1260

agugguuuca cgacauccca cucccguggc augccggcgc ugauaccgga acaccccacu     1320

ggaacaauaa ggaagcccuu guugaguuca aagacgcaca ugccaagagg cagaccgugg     1380

ucgugcuggg cagccaggaa ggggcugucc acacugcguu ggcaggagcc cuagaggcug     1440

agauggaugg ugccaaaggc cggcuguuuu ccgggcaucu caagugccga auuaagaugg     1500

acaaacugcg ccuuaaggga gugucuuaca gccuguguac ggcagccuuc acauuuucaa     1560

aaguuccugc ugaaaccuug cacggcacug ugaccguaga gguccaguau gccgguagag     1620

acgggccaug caaggugccc gcacaaaugg cgguugauau gcagacacuc accccugugg     1680

gcaggcugau aacugcuaac ccaaucauua cagaaaguac ggagaacucc cggaugaugu     1740

uagaacugga ucccccguuc ggagacagcu acaucgucau aggcgugggg gacaaaaaga     1800

uuacccacca uuggcaccgc ucuggaucaa cuaucgguaa ggccuuugag gcaacaguaa     1860

gaggcgccaa aaggauggcu gugcuagggg auaccgccug ggacuucgga aguguuggcg     1920

gugucuucaa uagccucggg aagggcgugc aucagguguu uggagcagcu uucaaauccc     1980

uguuuggcgg gaugucuugg uucucgcaaa uccuuauugg aacauugcug gucuggcucg     2040

gccugaacac uaagaauggu agcaucuccc uugccugucu ggcguuggga ggggccauga     2100

uuuuucucuc aaccgcuguu agugcaugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6286
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 2 opt

<400>  6286
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagauaucg      180

ugcuggaaca cggcgggugu gucacaguga uggcacaaga caaaccuacu guugauauug      240

aguuggugac gaccacaguc aguaauaugg ccgaagugcg gagcuacugc uaugaggcgu      300

ccaucucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guaugcaaac gaacacuggu ugaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuaua caaccggaga aucuggagua cagaaucaug cuuagcgucc      540

auggauccca gcacucaggu augauuguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu ggaaaucacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuagucc auaaggaaug guuucacgac auuccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuuguug      840

aguucaagga ugcacacgcc aagagacaga cagugguagu guugggcucc caggaagggg      900

cuguccauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ccccgcugag acccugcacg     1080

gcacuguuac aguggaaguc caauaugccg guaccgacgg gccuuguaaa gugccagcuc     1140

agauggccgu agauaugcag acguuaaccc cgguuggccg acugauaaca gcaaaucccg     1200

ugauuacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau cgucauuggc gugggggaaa agaaaaucac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgucagggg cgcgaaacgg auggcagugc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6287
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 2 opt

<400>  6287
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6288
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 2 opt

<400>  6288
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucggcu augaaaccga ugaggacagg gccaaggugg      600

aagucacgcc caacaguccu cgggcugagg caacucuggg aggguuuggc agcuuggguc      660

ucgauugcga gccacgcacc gggcuggacu ucucugaucu guacuaccuc acaaugaaua      720

acaaacauug guuaguucac aaggaauggu uucaugacau uccccugccu uggcacgccg      780

gagcugacac uggcacccca cacuggaaua acaaagaggc ccuuguggag uucaaggaug      840

cgcaugcaaa gagacagaca guaguggucu ugggcuccca ggaaggggcc gugcacaccg      900

cucuggccgg agcacuagaa gcugagaugg acggugccaa aggcaggcuc uuuucagggc      960

aucugaagug ucggcuuaaa auggauaagc ugcgcuugaa aggaguuagc uauagucucu     1020

gcacggcggc cuucacuuuu acaaaggugc ccgcagagac ccugcacggc acugucacag     1080

uggaaguaca auacgcuggu accgacgggc cuuguaaaau cccaguucag auggccgugg     1140

auaugcagac guuaaccccg gucggccgac ugauuacagc uaaucccgug aucacugagu     1200

cuaccgaaaa cuccaagaug augcucgagc uugacccucc auucggagau agcuauauag     1260

ucauuggcgu gggggacaag aaaaucacac accauuggca cagaucggga ucuacuauug     1320

guaaggccuu ugaagcaacc guuaggggcg ccaaacggau ggcugugcug ggggauacag     1380

cgugggacuu cggauccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6289
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 2 opt

<400>  6289
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6290
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 2 opt

<400>  6290
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauaccgg cuaugaaacg gacgagaaua      600

gggccaaggu cgaaguuacu cccaacaguc cucgggcuga ggcaacccug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca cagggcugga cuucucugau cuguacuacc      720

ucacuaugaa uaacaaacau ugguuagugc acaaggaaug guuucaugac auuccccugc      780

cuuggcacgc cggagcugac accggcacac cacacuggaa uaacaaagag gcccuuguag      840

aguucaagga ugcgcaugca aagagacaga ccguggucgu guugggcucc caggaagggg      900

ccguucacac ggcucuggcc ggagcacuag aagcugagau ggacggugcc aaaggcaagc      960

ucuuuucagg gcaucugaaa uguaggcuua agauggauaa acugcgguug aagggaguga     1020

gcuauagucu cugcacugcg gccuucacau uuaccaaagu ccccgcagag acucugcacg     1080

gcacagugac cguagaaguu caauacgcug guacggacgg gccuuguaag aucccagugc     1140

agauggccgu cgauaugcag accuuaacac cggugggccg ccugauuacu gcuaaucccg     1200

ucaucaccga gucuacagaa aacuccaaga ugaugcucga gcuugacccu ccauucggag     1260

auagcuauau agugauuggc guuggggaca aaaagaucac ucaccauugg caccgaucgg     1320

gaucuaccau ugguaaagcc uuugaagcaa cagugagagg cgccaagagg auggcuguac     1380

ugggggauac cgcgugggac uucggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6291
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 2 opt

<400>  6291
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaccguga acgauaucgg cuaugaaacg gacgagaaua      600

gggccaaggu cgaaguuacu cccaacaguc cucgggcuga ggcaacccug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca cagggcugga cuucucugau cuguacuacc      720

ucacuaugaa uaacaaacau ugguuagugc acaaggaaug guuucaugac auuccccugc      780

cuuggcacgc cggagcugac accggcacac cacacuggaa uaacaaagag gcccuuguag      840

aguucaagga ugcgcaugca aagagacaga ccguggucgu guugggcucc caggaagggg      900

ccguucacac ggcucuggcc ggagcacuag aagcugagau ggacggugcc aaaggcaagc      960

ucuuuucagg gcaucugaaa uguaggcuua agauggauaa acugcgguug aagggaguga     1020

gcuauagucu cugcacugcg gccuucacau uuaccaaagu ccccgcagag acucugcacg     1080

gcacagugac cguagaaguu caauacgcug guacggacgg gccuuguaag aucccagugc     1140

agauggccgu cgauaugcag accuuaacac cggugggccg ccugauuacu gcuaaucccg     1200

ucaucaccga gucuacagaa aacuccaaga ugaugcucga gcuugacccu ccauucggag     1260

auagcuauau agugauuggc guuggggaca aaaagaucac ucaccauugg caccgaucgg     1320

gaucuaccau ugguaaagcc uuugaagcaa cagugagagg cgccaagagg auggcuguac     1380

ugggggauac cgcgugggac uucggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6292
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 2 opt

<400>  6292
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgaugaaaa uagggccaag gucgagguua      600

cccccaacag uccucgggcu gaagcaacgc ugggcggguu uggcagcuug ggacucgacu      660

gcgagccacg cacuggucug gauuucucug accuguauua ccucaccaug aauaacaaac      720

auugguuagu gcacaaggag ugguuucaug auauuccccu gccuuggcac gccggggcug      780

acacaggaac uccacacugg aauaacaaag aagcccuugu agaguucaag gacgcgcaug      840

caaagagaca gaccgugguc guguugggcu cccaggaagg cgccguucac acagcucugg      900

ccggggcacu agaggcugaa auggauggag ccaaagguag gcucuuuuca ggccaucuga      960

agugucggcu uaaaauggac aagcugcgcu ugaaaggggu gagcuacagu cucugcaccg     1020

cggccuucac guuuacuaag guccccgcag agacacugca cggaaccgug acuguagagg     1080

uucaauaugc uggcacagau gguccuugua aagugccagc ccagauggcu gucgacaugc     1140

agaccuuaac gccggugggg cgacugauca ccgccaaucc cgucauuaca gaaucuacug     1200

agaacuccaa gaugaugcuc gaacuugauc cuccauucgg cgacagcuac aucgugauag     1260

gaguuggcga uaagaaaauu acccaccauu ggcacagauc ggggucuaca aucggaaagg     1320

cauuugaggc cacugugagg ggugcuaaac ggauggcggu acugggcgac accgcauggg     1380

auuucggguc cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  6293
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 2 opt

<400>  6293
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucggcu augaaaccga ugagaacagg gccaaggugg      600

aagucacgcc caauaguccu cgggcugagg caacucuggg aggguuuggc agcuuggguc      660

ucgacugcga gccacgcacc gggcuggauu ucucugaccu guacuaccuc acaaugaaca      720

auaaacauug guuaguucac aaggaauggu uucaugauau uccccugccu uggcacgccg      780

gagcugacac uggcacccca cacuggaaca auaaagaggc ccuuguggag uucaaggacg      840

cgcaugcaaa gagacagaca guaguggucu ugggcuccca ggaaggggcc gugcacaccg      900

cucuggccgg agcacuagaa gcugagaugg auggugccaa aggcaagcuc uuuucagggc      960

aucugaaaug uaggcuuaag auggacaaac ugcgguugaa gggaguuagc uauagucucu     1020

gcacggcggc cuucacuuuu acaaaagugc ccgcagagac ccugcacggc acugucacag     1080

uggaaguaca auacgcuggu accgaugggc cuuguaagau cccaguucag auggccgugg     1140

acaugcagac guuaaccccg gucggccgcc ugauuacagc uaaccccgug aucacugagu     1200

cuaccgaaaa uuccaagaug augcucgagc uugauccucc auucggagac agcuauauag     1260

ucauuggcgu gggggauaaa aagaucacac accauuggca ccgaucggga ucuacuauug     1320

guaaagccuu ugaagcaacc guuagaggcg ccaagaggau ggcugugcug ggggacacag     1380

cgugggauuu cggauccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6294
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 2 opt

<400>  6294
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc cugagaugca      120

ucggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

ucuuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauauug      240

aguuagucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccaucucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uaccuggaca      360

agcagagcga uacccaguau guuugcaaac gaacauuggu ggaccguggc ugggguaacg      420

gguguggacu cuuuggcaag ggcagucugg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauu caaccggaga aucuugagua cagaaucaug cugagcgucc      540

auggauccca gcacucaggu augauaguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaaucacg cccaacaguc cucgggcaga ggccacucua gggggauuug      660

gcagccucgg ucuggauugc gagccacgca ccgggcugga cuucucugau cucuauuacu      720

uaacaaugaa uaacaaacac uggcugguuc auaaggaaug guuucacgac auuccccuuc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcguuggugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu ccugggcucc caggaagggg      900

cugugcauac cgcccuagca ggagcucucg aagccgagau ggacggugcg aaaggcaggc      960

ugucaagcgg gcaccuuaag ugucggcuga aaauggauaa guugcgccuc aaaggaguua     1020

guuacucucu gugcacggcc gcauuuacuu ucacaaagau ccccgcugag accuuacacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acgcugaccc cggugggccg acucauuaca gcaaaucccg     1200

ucaucacuga guccaccgaa aacagcaaga ugaugcuuga gcuggacccu ccauuuggag     1260

auucguacau agugauuggc gucggggaaa agaaaaucac acaucacugg cauagaucug     1320

gauccacuau ugguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuu     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6295
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 2 opt

<400>  6295
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagca       60

agaaagugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacagga caagccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca aggagaagcc uacuuagaca      360

aacagagcga uacccaguau guuugcaagc gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaaa ggcagucucg ugaccugcgc aaaguucgcc uguuccaaga      480

aaaugacugg gaagucuauc cagccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca acacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaagu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaagcac ugguuaguuc auaaagaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaggag gcgcuugugg      840

aguucaagga ugcacacgcc aaaagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aagggcaggc      960

ucucaagcgg gcaccugaaa ugucggcuua agauggauaa acugcgcuug aagggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaaaau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caguaugccg guaccgacgg gccuuguaag guaccagcuc     1140

aaauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaga aaaagauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaagcc uucgaagcua ccgugagggg cgcgaagcgg auggcaguuc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6296
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 2 opt

<400>  6296
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac acaucacugg uauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6297
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 2 opt

<400>  6297
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaagc      960

ucucaagcgg gcaccugaaa uguaggcuua agauggauaa acugcgguug aagggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaaaau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaag guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg ccugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaga aaaagauuac acaucacugg caucgaucug     1320

gauccacuau cgguaaagcc uucgaagcua ccgugagagg cgcgaagagg auggcaguuc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6298
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 2 opt

<400>  6298
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauacgcag accuuaacac cggugggccg acugaucacu gcaaaucccg     1200

ucauaaccga guccacagaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac ucaucacugg cauagaucug     1320

gauccaccau cgguaaggcc uucgaggcua cagugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac cgccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6299
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 2 opt

<400>  6299
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaauucg gagcuacugc uaugaggcgu      300

ccaucucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guaugcaaac gaacacuggu ugaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuaua caaccggaga aucuggagua cagaaucaug cuuagcgucc      540

auggauccca gcacucaggu augauuguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu ggaaaucacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuagucc auaaggaaug guuucacgac auuccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuuguug      840

aguucaagga ugcacacgcc aagagacaga cagugguagu guugggcucc caggaagggg      900

cuguccauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ccccgcugag acccugcacg     1080

gcacuguuac aguggaaguc caauaugccg guaccgacgg gccuuguaaa gugccagcuc     1140

agauggccgu agauaugcag acguuaaccc cgguuggccg acugauaaca gcaaaucccg     1200

ugauuacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau cgucauuggc gugggggaaa agaaaaucac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgucagggg cgcgaaacgg auggcagugc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6300
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 2 opt

<400>  6300
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgcggcgu auggcaguuc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6301
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 2 opt

<400>  6301
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga caugguuucg gauucacgcu guccaacuca gggagaggcu uacuuagaca      360

agcagagcga uacccaguau gugugcaaac gaacacuggu cgaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc caaauucgca uguuccaaga      480

agaugacugg gaaaucuauc caaccggaaa aucuggagua cagaauaaug cuuagcgugc      540

auggauccca gcacucaggu augaucguca acgauacagg ccaugaaacc gacgagaaua      600

gggccaaggu ugaaauuacg cccaacaguc cucgggcuga ggcaacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuagugc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc cggagcugac acuggcaccc cacauuggaa uaacaaagag gcccuuguag      840

aguucaagga ugcgcacgca aagagacaga caguggucgu guugggcucc caggaagggg      900

ccguucauac cgcucuggcc ggagcacuag aagcugagau ggacggugcc aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

guuacucucu cugcacggcg gccuuuacuu ucacaaagau ucccgcagag acccugcacg     1080

gcacugucac aguggaagua caauaugcug guaccgacgg gccuuguaaa guuccagccc     1140

agauggcugu ggauaugcag acguuaaccc cggucggccg acugaucaca gccaaucccg     1200

ugauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugucaucggc gugggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggca uucgaggcca ccguuagggg cgcuaaacgg auggcggugc     1380

ugggggacac agcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6302
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 2 opt

<400>  6302
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccauugaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau cgugauuggc gucggggaaa agaaaaucac ccaucacugg cauagaucug     1320

gauccacaau cgguaaggcc uucgaggcua cugugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac cgccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6303
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 2 opt

<400>  6303
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc auuggggaaa agaaaaucac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgucagggg cgcgaaacgg auggcagugc     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6304
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 2 opt

<400>  6304
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccu ggcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

ucuuggaaca cggcgggugu gugacaguua uggcacaaga caaacccacu guggauaucg      240

aguuagucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccuacuca gggagaagcc uaccuggaca      360

agcagagcga uacccaguau guuugcaaac gaacauuggu ggaccguggc ugggguaacg      420

gguguggacu cuuuggcaag ggcagucugg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccagaga aucuugagua cagaauaaug cugagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg ccgaacaguc cccgggcaga ggccacucua gggggauuug      660

gcagccucgg ucuggauugc gagccucgca ccgggcugga cuucucugau cucuauuacu      720

uaacaaugaa uaacaaacac uggcugguuc auaaggaaug guuucacgac aucccacuuc      780

ccuggcacgc uggagccgac acuggcaccc cucauuggaa uaacaaagag gcguuggugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu ccugggcucc caggaagggg      900

cugugcauac cgcccuagca ggagcucucg aagccgagau ggacggugcg aaaggcaggc      960

ugucaagcgg gcaccuuaag ugucggcuga aaauggauaa guugcgccuc aaaggaguua     1020

guuacucucu gugcacggcc gcauuuacuu ucacaaagau uccagcugag accuuacacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gcccuguaaa guaccugcuc     1140

agauggccgu ugauaugcag acgcugaccc cagugggccg acucaucaca gcaaauccgg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcuuga gcuggauccc ccuuuuggag     1260

acucguacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuu     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6305
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 2 opt

<400>  6305
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcacc caggaagggg      900

cugugcauac ggcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucuccucagg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

gcuacagucu cugcacugcc gcauuuacau ucaccaagau ucccgcugag acucugcacg     1080

gcacagugac cgucgaagug caauaugccg guacggacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag accuuaacac cggugggccg acugaucacu gcaaaucccg     1200

ucauaaccga gucuacagaa aacuccaaga ugaugcucga gcuugacccu ccauuuggag     1260

auagcuacau ugugaucggc gucggggaaa agaaaauuac ucaucacugg cauagaucgg     1320

gaucuaccau cgguaaggcc uucgaggcua cagugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac cgccugggau uuuggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6306
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 2 opt

<400>  6306
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guauuagcuc     1140

agauggccgu ugauaugcag acgcugaccc cagugggccg acucaucaca gcaaauccgg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcuuga gcuggauccc ccuuuuggag     1260

acucguacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuu     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6307
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 2 opt

<400>  6307
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga agcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aggccgagau ggauggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggacaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugaa acccugcacg     1080

gcacugugac agucgaggug caauaugccg guaccgaugg gccuuguaaa guaccagcuc     1140

agauggccgu ugacaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga auccaccgag aacagcaaga ugaugcucga acuugauccu ccauuuggag     1260

acucguacau ugugaucggc gucggggaga agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuc     1380

ugggggauac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6308
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 2 opt

<400>  6308
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccgcucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca cugggcugga cuucucugau cuguauuacc      720

ucaccaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc cggagcggac acaggcacuc cacauuggaa uaacaaagag gcacuugugg      840

aguucaagga ugcccacgcu aagagacaga ccguaguggu cuugggcucc caggaagggg      900

ccgugcauac agcacuggcu ggagcccuag aagcggagau ggacggugcc aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcaccgca gcuuuuacgu ucacuaagau ucccgccgag acacugcacg     1080

gcaccgugac ugucgaagug caauaugcug guacagacgg gccuuguaaa guaccagccc     1140

agauggcagu ugauaugcag accuuaacgc cggugggccg acugaucacc gccaaucccg     1200

ucauaacaga guccacugaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaucug     1320

gauccacaau cgguaaggcu uucgaggcga cugugagggg cgcaaaacgg auggccguuc     1380

ugggggacac cgcuugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6309
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 2 opt

<400>  6309
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucag uacgaccgug agcaauaugg ccgaaguacg guccuacugc uaugaggcga      300

caauuucuga caucgcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuaua caaccggaga aucuggagua cagaaucaug cuuagcgucc      540

auggauccca gcacucaggu augauuguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaaucacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac auuccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga cagcuguagu guugggcucc caggaagggg      900

ccguccauac cgcacuggcu ggagcccuag aagcggagau ggacggugcc aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

guuacucucu cugcacggca gcuuuuacuu ucacaaagau ccccgccgag acccugcacg     1080

gcacuguuac aguggaaguc caauaugcug guaccgacgg gccuuguaaa gugccagccc     1140

agauggcagu agauaugcag acguuaaccc cgguuggccg acugauaaca gccaaucccg     1200

ugauuacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau cgucauuggc gugggggaaa agaaaaucac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcu uucgaggcga ccgucagggg cgcaaaacgg auggccgugc     1380

ugggggacac agcuugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6310
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 2 opt

<400>  6310
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucuccaagau ucccgcugag acacugcacg     1080

gcaccgugac ugaagagguc caauaugccg guacagacgg gccuuguaaa gugccagcuc     1140

agauggccgu agauaugcag accuuaacgc cgguuggccg acugaucacc gcaaaucccg     1200

ugauaacaga aagcacugag aacucgaaga ugaugcucga acuugacccu ccauuuggag     1260

auucuuacau ugucaucggc gugggggaga agaaaauuac ccaucacugg cauagauccg     1320

gaucaacaau cgguaaggcc uucgaggcua cugucagggg cgcgaaacgg auggcagugc     1380

ugggggacac cgccugggau uuuggaaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6311
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 2 opt

<400>  6311
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaauua caauaugccg guaccgacgg gccuuguaaa gugccagcuc     1140

agauggccgu agauaugcag acgcugaccc cgguuggccg acucaucaca gcaaaucccg     1200

ugauaacuga guccaccgaa aacagcaaga ugaugcuuga gcuggacccu ccauuuggag     1260

auucguacau ugucaucggc gugggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggcc uucgaggcua ccgucagggg cgcgaaacgg auggcagugu     1380

ugggggacac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6312
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 2 opt

<400>  6312
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcaucuc uaacagggac uucguugagg ggaugucagg agguaccugg guggauguag      180

ugcuggaaca cggcgggugu gucacaguga uggcacaaga caaaccuacu guugauauug      240

aguuggugac gaccacaguc aguaauaugg ccgaagugcg gagcuacugc uaugaggcgu      300

ccaucucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guaugcaaac gaacacuggu ugaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuaua caaccggaga aucuggagua cagaaucaug cuuagcgucc      540

auggauccca gcacucaggu augauuguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu ggaaaucacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcauuuuggg ucucgauugc gagccacgca ccgggcugga cuucagcgau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuagucc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuuguug      840

aguucaagga ugcacacgcc aagagacaga cagugguagu guugggcucu caggaagggg      900

cuguccauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucuccucagg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

gcuacagucu cugcacggcc gcauuuacuu ucacaaagau acccgcugag acccugcacg     1080

gcacuguuac aguggaaguc caauaugccg guaccgacgg gccuuguaaa gugccagcuc     1140

agauggccgu agauaugcag acguuaaccc cgguuggccg acugauuaca gcaaaucccg     1200

ugaucacuga gucuaccgaa aacuccaaga ugaugcucga gcuugacccu ccauuuggag     1260

auagcuacau ugucaucggc gugggggaaa agaaaaucac acaucacugg cauagaucgg     1320

gaucuacuau ugguaaggcc uucgaggcua ccgucagggg cgcgaaacgg auggcagugc     1380

ugggggacac agccugggau uuuggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6313
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 2 opt

<400>  6313
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacgguguu aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

guuacucucu cugcacggcg gccuuuacuu ucacaaagau ucccgcagag acccugcacg     1080

gcacugucac aguggaagua caauaugcug guaccgacgg gccuuguaaa guuccagccc     1140

agauggcugu ggauaugcag acguuaaccc cggucggccg acugaucaca gccaaucccg     1200

ugauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugucaucggc gugggggaaa agaaaauuac acaucacugg cauagaucug     1320

gauccacuau cgguaaggca uucgaggcca ccguuagggg cgcuaaacgg auggcggugc     1380

ugggggacac agcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6314
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 2 opt

<400>  6314
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg gugcugacgg gccuuguaaa guaccagccc     1140

agauggcagu ugauaugcag accuuaacgc cggugggccg acugaucacc gccaaucccg     1200

ucauaacaga guccacugaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaucug     1320

gauccacaau cgguaaggcu uucgaggcga cugugagggg cgcaaaacgg auggccguuc     1380

ugggggacac cgcuugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6315
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 2 opt

<400>  6315
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacac uggagcugac accggcacac cacauuggaa uaacaaagag gcccuugugg      840

aguucaagga ugcgcacgca aagagacaga ccguaguggu cuugggcucc caggaagggg      900

ccgugcauac ggcucuggcc ggagcacuag aagcugagau ggacggugcc aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacugcg gccuuuacau ucaccaagau ucccgcagag acucugcacg     1080

gcacagugac cgucgaagug caauaugcug guacggacgg gccuuguaaa guaccagccc     1140

agauggcugu ugauaugcag accuuaacac cggugggccg acugaucacu gccaaucccg     1200

ucauaaccga gggaacagaa aacuccaaga ugaugcucga gcuugacccu ccauuuggcg     1260

auagcuacau ugugaucggg gucggagaaa agaaaauuac ucaucacugg cauagaucgg     1320

guucuaccau cggcaaggca uucgaggcca cagugagggg agcuaaacgg auggcgguuc     1380

ugggggacac cgcaugggau uuuggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6316
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 2 opt

<400>  6316
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacagcaa uggcccaaga caaaccuacu guugauaucg      240

aguuggugac gaccacaguc aguaauaugg cggaagugcg gagcuacugc uaugaggcuu      300

ccauuucuga cauggccucg gauucacgcu guccaacuca gggagaagca uacuuagaca      360

agcagagcga uacccaguau guaugcaaac gaacacuggu ugaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc caaauucgcu uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augcugguga acgauacagg ccaugaaacc gacgagaaua      600

gggcaaaggu ggaaaucacg cccaacaguc cucgggccga ggcuacucuc gggggauuug      660

gcagcuuggg ucuggauugc gagccacgca ccgggcugga cuucucugau cucuauuacu      720

uaacaaugaa uaacaaacac uggcuggccc auaaggaaug guuucacgac auuccccuuc      780

cuuggcacgc gggagcagcc acuggcaccc cacauuggaa uaacaaagag gcuuuggucg      840

aguucaagga cgcccacgca aagagacaga caguuguggu acugggcucc caggaagggg      900

cugugcauac cgcccuagcg ggagcccucg aagcagagau ggauggugcu aaaggcaggc      960

ugucaagcgg gcaccuuaag ugucggcuga aaauggacaa guugcgccuc aaaggaguca     1020

guuacucucu gugcacggcc gcuuuuacuu ucacaaagau ccccgccgag accguggaug     1080

gcacuguuac aguggaaggu caauaugggg gcaccgacgg accuuguaaa gucccagcac     1140

agauggccgu ggauaugcag acguuaaccc cgguaggccg acugauuaca gcuaaucccg     1200

uuaucacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuugggg     1260

auucguacau agugauugga gucggugaaa agaaaaucac acaccauugg cacagaucug     1320

gcuccacuau ugggaaggcg uucgaggcaa ccgugagggg agccaaacgg auggcugucc     1380

ugggcgacac agccugggau uuugguucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6317
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 2 opt

<400>  6317
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccugcc guggauaucg      240

aguuggucac uacgaccgug aguaauaugg cggaaguacg gagcuacugc uaugaggcuu      300

ccauuucuga cauggccucg gauucacgcu guccaacaca gggagaagca uacuuagaca      360

agcagagcga uacucaguau guuugcaaac gaacccuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ucacaugcgc caaauucgcu uguuccaaga      480

agaugaccgg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgugc      540

auggauccca gcacucaggu augaucguga acgauacugg ccaugaaaca gacgagaaua      600

gggcaaaggu cgaaauuacc cccaacaguc cucgggccga ggcuacgcug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca cugggcugga cuucucugau cuguauuacc      720

ucaccaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc cggagcggac acaggcacuc cacauuggaa uaacaaagag gcacuugugg      840

aguucaagga ugcccacgcu aagagacaga ccguaguggu cuugggcucc caggaagggg      900

ccgugcauac agcacuggcu ggagcccuag aagcggagau ggacggugcc aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcaccgca gcuuuuacgu ucacuaagau ucccgccgag acacugcacg     1080

gcaccgugac ugucgaagug caauaugcug guacagacgg gccuuguaaa guaccagccc     1140

agauggcagu ugauaugcag accuuaacgc cggugggccg acugaucacc gccaaucccg     1200

ucauaacaga guccacugaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaucug     1320

gauccacaau cgguaaggcu uucgaggcga cugugagggg cgcaaaacgg auggccguuc     1380

ugggggacac cgcuugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6318
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 2 opt

<400>  6318
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuaa caauagggac uucguggagg ggaugucugg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug ucaaacaugg ccgaaguacg gaguuacugc uaugaggcga      300

gcauuuccga cauggcuucu gauucgcgcu guccaacuca gggagaagcc uacuuagaca      360

agcagucaga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaaug      420

gguguggacu auuuggcaag ggcagccucg ugaccugcgc aaaauucgcc uguaguaaga      480

agaugacugg gaaauccauc caaccggaga accuggagua cagaauaaug cuuucugucc      540

auggaagcca gcacuccggu augaucguga augauacagg ccaugaaacc gacgagaaca      600

gggcuaaggu cgaaauuacg cccaauucac cucgggcaga ggccacucug ggaggguuug      660

gcaguuuggg ucucgauugc gagccacgca ccgggcugga cuucagcgau cuguauuacc      720

ucacaaugaa caauaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa caauaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucu caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucuccucagg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

gcuacagucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaccccg     1200

ucauaacuga gucuaccgaa aauuccaaga ugaugcucga gcuugacccu ccauuuggag     1260

auagcuacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauagaucgg     1320

gaucuacuau cgguaaggcc uucgaggcua ccgugagggg cgcgaaacgg auggcaguuc     1380

ugggggacac agccugggau uuuggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6319
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 2 opt

<400>  6319
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcgccgca gcuuuuacgu ucacuaagau ucccgccgag acacugcacg     1080

gcaccgugac ugucgaagug caauaugcug guacagacgg gccuuguaaa guaccagccc     1140

agauggcagu ugauaugcag accuuaacgc cggugggccg acugaucacc gccaaucccg     1200

ucauaacaga guccacugaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaucug     1320

gauccacaau cgguaaggcu uucgaggcga cugugagggg cgcaaaacgg auggccguuc     1380

ugggggacac cgcuugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6320
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 2 opt

<400>  6320
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauauugg ccaugaaaca gacgagaaua      600

gggcuaaggu cgaaaucacc cccaacaguc cucgggcaga ggccacgcug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca cugggcugga cuucucugau cuguauuacc      720

ucaccaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac auuccccugc      780

cuuggcacgc uggagccgac acaggcacuc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga ccguaguggu cuugggcucc caggaagggg      900

cugugcauac agcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcaccgcc gcauuuacgu ucacuaagau ccccgcugag acacugcacg     1080

gcaccgugac ugucgaagug caauaugccg guacagacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag accuuaacgc cggugggccg acugauaacc gcaaaucccg     1200

ucauuacaga guccacugaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau cgugauuggc gucggggaca agaaaaucac ccaucacugg cauagaucug     1320

gauccacaau cgguaaggcc uucgaagcua cugugagggg cgcgaaacgg auggcaguuc     1380

ugggggauac cgccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6321
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 2 opt

<400>  6321
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucgcc uguuccaaga      480

agaugacugg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauacagg ccaugaaacc gacgagaaua      600

gggcuaaggu cgaaauuacg cccaacaguc cucgggcaga ggccacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagagacaga caguaguggu cuugggcucc caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggcaggc      960

ucucaagcgg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguua     1020

guuacucucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggccg acugaucaca gcaaaucccg     1200

ucauaacuga guccaccgaa aacagcaaga ugaugcucga gcuugacccu ccauuuggag     1260

auucguacau ugugaucggc gucggggaca agaaaauuac acaucacugg uauagaucug     1320

gauccacuau cgguaaggcc uucgaagcua ccgugagggg cgcgaaacgg auggcaguuc     1380

ugggggauac agccugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6322
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 2 opt

<400>  6322
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauaccgg ccaugaaacg gacgagaaua      600

gggccaaggu cgaaguuacu cccaacaguc cucgggcuga ggcaacccug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca cagggcugga cuucucugau cuguauuacc      720

ucacuaugaa uaacaaacac ugguuagugc auaaggaaug guuucacgac auuccccugc      780

cuuggcacgc cggagcugac accggcacac cacauuggaa uaacaaagag gcccuuguag      840

aguucaagga ugcgcacgca aagagacaga ccguggucgu guugggcucc caggaagggg      900

ccguucauac ggcucuggcc ggagcacuag aagcugagau ggacggugcc aaaggcaggc      960

ucuuuucagg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

gcuacagucu cugcacugcg gccuucacau uuaccaaggu ccccgcagag acucugcacg     1080

gcacagugac cguagaaguu caauaugcug guacggacgg gccuuguaaa gugccagccc     1140

agauggcugu cgauaugcag accuuaacac cggugggccg acugaucacu gccaaucccg     1200

ucauuaccga gucuacagaa aacuccaaga ugaugcucga gcuugacccu ccauucggag     1260

auagcuacau cgugauaggc guuggggaca agaaaauuac ucaucacugg cauagaucgg     1320

gaucuaccau cgguaaggca uuugaagcca cagugagggg cgcuaaacgg auggcgguac     1380

ugggggauac cgcaugggac uucggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6323
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 2 opt

<400>  6323
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauauugg ccaugaaacc gacgagaaua      600

gggccaaggu cgaaguuacg cccaacaguc cucgggcuga ggcaacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuagugc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc cggagcugac acuggcaccc cacauuggaa uaacaaagag gcccuuguag      840

aguucaagga ugcgcacgca aagagacaga caguggucgu guugggcucc caggaagggg      900

ccguucauac cgcucuggcc ggagcacuag aagcugagau ggacggugcc aaaggcaggc      960

ucuuuucagg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

gcuacagucu cugcacggcg gccuucacuu uuacaaaggu ccccgcagag acccugcacg     1080

gcacugugac aguagaaguu caauaugcug guaccgacgg gccuuguaaa gugccagccc     1140

agauggcugu cgauaugcag acguuaaccc cggugggccg acugauuaca gccaaucccg     1200

ucaucacuga gucuaccgaa aacuccaaga ugaugcucga gcuugacccu ccauucggag     1260

auagcuacau agugauuggc guuggggaca agaaaaucac acaucacugg cauagaucgg     1320

gaucuacuau ugguaaggca uuugaagcca ccgugagggg cgcuaaacgg auggcgguac     1380

ugggggauac agcaugggac uucggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6324
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 2 opt

<400>  6324
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauauugg ccaugaaacc gacgagaaua      600

gggccaaggu cgaaguuacg cccaacaguc cucgggcuga ggcaacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuagugc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc cggagcugac acuggcaccc cacauuggaa uaacaaagag gcccuuguag      840

aguucaagga ugcgcacgca aagagacaga caguggucgu guugggcucc caggaagggg      900

ccguucauac cgcucuggcc ggagcacuag aagcugagau ggacggugcc aaaggcaggc      960

ucuuuucagg gcaccugaag ugucggcuua aaauggauaa gcugcgcuug aaaggaguga     1020

gcuacagucu cugcacggcg gucuguacug ccgcaaaggu gcccgcugag acacugcacg     1080

gcaccguaac uguugaagug caauaugccg guacagacgg gccuugcaaa gucccagcuc     1140

agauggccgu ggauaugcag accuuaacgc cggucggccg acugauuacc gcaaaucccg     1200

ugaucacaga gucuacugaa aacuccaaga ugaugcucga gcuugacccu ccauucggag     1260

auagcuacau aguuauuggc gugggggaca agaaaaucac ccaucacugg cauagaucgg     1320

gaucuacaau ugguaaggcc uuugaagcua cuguaagggg cgcgaaacgg auggcagucc     1380

ugggggauac cgccugggac uucggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6325
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 2 opt

<400>  6325
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucagaugca      120

uuggcguuuc uaacagggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg gagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgcu guccaacuca gggagaagcc uacuuagaca      360

agcagagcga uacccaguau guuugcaaac gaacacuggu ggaccguggc ugggguaacg      420

gguguggacu auuuggcaag ggcagucucg ugaccugcgc aaaauucacu uguuccaaga      480

agaugacagg gaaaucuauc caaccggaga aucuggagua cagaauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgaugaaaa uagggccaag gucgagguua      600

cccccaacag uccucgggcu gaagcaacgc ugggcggguu uggcagcuug ggacucgacu      660

gcgagccacg cacuggucug gauuucucug accuguauua ccucaccaug aauaacaaac      720

auugguuagu gcacaaggag ugguuucaug auauuccccu gccuuggcac uccggggccg      780

acacagaaac uccacacugg aauaacaaag aggcucuugu agaguucaag gacgcccaug      840

cgaagagaca gaccgugguc guguugggau cacaggaagg cgcaguucac acagcccugg      900

cuggcgcccu agaagcagag auggaugggg cuaaaggaag gcucagcagu ggucaucuga      960

agugucggcu uaaaauggac aagcugcgcu ugaaaggcgu gucuuacucc cucugcaccg     1020

ccgcguuuac guucacuaag guccccgccg agacacugca cgggaccgug acuguagaag     1080

uucaauaugc aggacgagau ggcccuugua aagugccagc ucagauggcc gucgacaugc     1140

agacauuaac cccggugggu agacugauca cggcuaaucc cgucauuacc gagagcacag     1200

aaaacucgaa gaugaugcuc gagcuugauc cuccauuugg ggacucuuac aucgugauag     1260

gcguuggaga uaagaaaauu acucaccauu ggcacagguc cggcucaauc auugggaagg     1320

ccuucgaagc aaccgugcgg ggagccaaac guauggcugu acugggugac acagcguggg     1380

auuuuggcag uugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  6326
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 2 opt

<400>  6326
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauuguuc ucauagugcc cgccuauucc aucucuugca      120

uuggcguauc aaacagagac cuuguggagg ggaugagugg agguaccugg gucgaugugg      180

uucuggaaca cggcgggugu gugacagaga uggcucaaga caaaccuacu gucgauaucg      240

aauuggugac gaugaccgua agcaauaugg cagagguuag guccuacugc uaugaagccu      300

cuuuaucgga cauggcguca gcuagccggu guccaacaca gggagagccg agucuggaua      360

agcaguccga cacucagucu gugugcaaac gcacccuagg cgaucgaggu ugggggaacg      420

gauguggcau uuucggcaag gggagccucg ucacaugcuc caaauuuacc uguugcaaga      480

agaugcccgg aaaaucaauc caaccugaga aucuggaaua ccguauaaug cuuccagugc      540

augguaguca gcacagcggc augaucguga acgacauugg gcaugagacu gaugaaaaua      600

gagccaaggu cgagguuaca cccaacucuc cuagggcaga ggccacccug ggaggcuucg      660

guuccuuggg gcucgacugu gaaccacgga cgggacugga uuuuucagac cuguauuacc      720

ucacuaugaa uaacaaacac ugguuagugc auaaggagug guuccacgau aucccccugc      780

cuuggcacgc uggcgcagac accggcacac cacauuggaa uaacaaagaa gcccuuguag      840

aguuuaagga cgcucacgcc aagcgccaga cuguggucgu guuggggagc caggaaggag      900

cgguucauac cgcacuggcc ggugcucuag aggccgagau ggauggcgca aaagggagac      960

ucuucagugg acaccugaag ugcaggcuua aaauggacaa gcugcgguug aaaggcgugu     1020

cuuauucccu cuguacagcu gccuuuaccu ucacgaaggu cccggcggaa acucugcacg     1080

guacagugac cguagagguu caaagcgccg ggacugaugg ccccugcaaa gugccugcac     1140

agauggcugu cgacaugcag acauuaaccc cagugggacg ccugauuacg gccaaucccg     1200

ucaucaccga aucgacagag aacucuaaga ugaugcucga acuugauccu ccauuuggcg     1260

acuccuacau agugauuggg guuggagaua agaaaaucac ucaucacugg caucgaucag     1320

guaguaccau uggcaaggcu uucgaggcca cagugagagg ggcaaaaagg auggccguac     1380

ugggagacac ugcuugggau uuuggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6327
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 2 opt

<400>  6327
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

agaaggugau cuaccugguc augauucucc uuauagcccc cgcuuauagc aucaggugca      120

uuggcguuuc caaccgggac uucguggagg ggaugucugg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caaaccuacu guggauaucg      240

aguuggucac gaccacagug ucaaauaugg ccgaaguacg caguuacugc uaugaggcga      300

gcauuuccga cauggcuucu gauucgcgau guccaacuca gggagaagcc uacuuagaca      360

agcagucaga uacccaguau guuugcaaac guacacuggu ggacagaggc ugggguaacg      420

gguguggacu auuuggcaag ggcagccucg ugaccugcgc aaaauucgcc uguaguaaga      480

agaugacugg gaaauccauc caaccggaga aucuggagua caggauaaug cuuucugucc      540

auggaagcca gcacuccggu augaucguga acgauacagg ccaugaaacc gacgagaauc      600

gggcuaaggu cgaaauuacg cccaacucac cucgcgcaga ggccacucug ggaggguuug      660

gcaguuuggg ucucgauugc gagccaagaa ccgggcugga cuucagcgau cuguauuacc      720

ucacaaugaa uaacaaacac ugguuaguuc auaaggaaug guuucacgac aucccccugc      780

cuuggcacgc uggagccgac acuggcaccc cacauuggaa uaacaaagag gcgcuugugg      840

aguucaagga ugcacacgcc aagaggcaga caguaguggu cuugggcucu caggaagggg      900

cugugcauac cgcccuggca ggagcucuag aagccgagau ggacggugcg aaaggccggc      960

ucuccucagg gcaccugaag ugucgccuua aaauggauaa gcugcgauug aaaggaguua     1020

gcuacagucu cugcacggcc gcauuuacuu ucacaaagau ucccgcugag acccugcacg     1080

gcacugugac agucgaagug caauaugccg guaccgacgg gccuuguaaa guaccagcuc     1140

agauggccgu ugauaugcag acguuaaccc cggugggcag acugaucaca gcaaaucccg     1200

ucauaacuga gucuaccgaa aacuccaaga ugaugcucga gcuugacccu ccauuuggag     1260

auagcuacau ugugaucggc gucggggaaa agaaaauuac acaucacugg cauaggucgg     1320

gaucuacuau cgguaaggcc uucgaggcua ccgugcgggg cgcgaaacgu auggcaguuc     1380

ugggggacac agccugggau uuuggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6328
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 2 opt

<400>  6328
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agagagugau cuaccugguc augauucucc uuauagcccc cgcuuauucc aucaggugca      120

uuggcguuuc uaaccgggac uucguggagg ggaugucagg agguaccugg guagaugugg      180

uccuggaaca cggcgggugu gugacaguua uggcacaaga caagccuacu guggauaucg      240

aguuggucac gaccacagug aguaauaugg ccgaaguacg cagcuacugc uaugaggcgu      300

ccauuucuga cauggcuucg gauucacgau guccaacuca gggagaagcc uacuuagaca      360

aacagagcga uacccaguau guuugcaagc guacacuggu ggacagaggc ugggguaacg      420

gguguggacu auuuggcaaa ggcagucucg ugaccugcgc aaaguucacu uguuccaaaa      480

agaugacagg gaagucuauc caaccggaga aucuggagua caggauaaug cuuagcgucc      540

auggauccca gcacucaggu augaucguga acgauauugg ccaugaaacc gacgagaauc      600

gggccaaagu cgaaguuacg cccaacaguc cucgcgcuga ggcaacucug ggaggguuug      660

gcagcuuggg ucucgauugc gagccaagaa ccgggcugga cuucucugau cuguauuacc      720

ucacaaugaa uaacaagcac ugguuagugc auaaagaaug guuucacgac aucccccugc      780

cuuggcacgc cggagcugac acuggcaccc cacauuggaa uaacaaggag gcccuuguag      840

aguucaaaga ugcgcacgca aagaggcaga caguggucgu guugggcucc caggaagggg      900

ccguucauac cgcucuggcc ggagcacuag aagcugagau ggacggugcc aagggccggc      960

ucuuuucagg gcaccugaaa ugucgcauua agauggauaa acuucgacug aagggaguga     1020

gcuacaguuu gugcacggcg gccuucacuu uuucuaaagu ccccgcagag acacuccacg     1080

gcaccgugac uguagaaguu caauaugcug guagagacgg gccuuguaag gugccagccc     1140

agauggcugu cgauaugcag acacugaccc cggugggcag guuaaucacg gccaauccca     1200

uaauuaccga guccacagaa aacagccgga ugaugcugga gcucgacccu ccauucggag     1260

auucguacau cgucauuggc gugggggaca aaaagaucac ucaucacugg caucguucug     1320

gauccaccau cgguaaggca uuugaagcca caguucgcgg cgcuaaaaga auggcggugc     1380

uuggggauac ugcaugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6329
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  6329
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6330
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 opt

<400>  6330
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaccug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6331
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opt

<400>  6331
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggacaga gccaaggugg     1140

aggugacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug uucagcggcc     1500

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1680

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgugu ucaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcuggugugg cugggccuga acaccaagaa cggcagcauc agccugaccu     2100

gccuggcccu gggcggcgug augaucuucc ugagcaccgc cgugagcgcc gacgugggcu     2160

gcagcgugga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6332
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 2 opt

<400>  6332
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6333
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  6333
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6334
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 2 opt

<400>  6334
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac accugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaacg     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6335
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 2 opt

<400>  6335
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cggcuacgag accgacgagg     1080

acagagccaa gguggaggug acccccaaca gccccagagc cgaggccacc cugggcggcu     1140

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu     1200

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1380

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1440

gacuguucag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1500

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aagauccccg     1620

ugcagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1680

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1740

gcgacagcua caucgugauc ggcgugggcg acaagaagau cacccaccac uggcacagaa     1800

gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag agaauggccg     1860

ugcugggcga caccgccugg gacuucggca gcgugggcgg cguguucaac agccugggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc augagcuggu     1980

ucagccagau ccugaucggc acccugcugg uguggcuggg ccugaacacc aagaacggca     2040

gcaucagccu gaccugccug gcccugggcg gcgugaugau cuuccugagc accgccguga     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6336
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 2 opt

<400>  6336
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6337
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 opt

<400>  6337
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6338
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 opt

<400>  6338
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6339
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opt

<400>  6339
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggacaga gccaaggugg      600

aggugacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc      660

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca      720

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg      780

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg      840

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg      900

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug uucagcggcc      960

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1080

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1140

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1200

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1260

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1320

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6340
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 2 opt

<400>  6340
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6341
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       2 opt

<400>  6341
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccugagcg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6342
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 2
       opt

<400>  6342
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac accugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccugagcg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaacg     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6343
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 2 
       opt

<400>  6343
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccugagcg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cggcuacgag accgacgagg     1080

acagagccaa gguggaggug acccccaaca gccccagagc cgaggccacc cugggcggcu     1140

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu     1200

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1380

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1440

gacuguucag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1500

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aagauccccg     1620

ugcagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1680

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1740

gcgacagcua caucgugauc ggcgugggcg acaagaagau cacccaccac uggcacagaa     1800

gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag agaauggccg     1860

ugcugggcga caccgccugg gacuucggca gcgugggcgg cguguucaac agccugggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc augagcuggu     1980

ucagccagau ccugaucggc acccugcugg uguggcuggg ccugaacacc aagaacggca     2040

gcaucagccu gaccugccug gcccugggcg gcgugaugau cuuccugagc accgccguga     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6344
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 2 
       opt

<400>  6344
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccugagcg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6345
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       2 opt

<400>  6345
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugcaggac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6346
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 2
       opt

<400>  6346
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac accugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugcaggac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaacg     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6347
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 2 
       opt

<400>  6347
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugcaggac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6348
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6348
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc acccugggca aggccuucag caccacccug aagggcgccc     1860

agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc ggcguguuca     1920

acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc cuguucggcg     1980

gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg     2040

ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6349
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6349
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac accugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc acccugggca aggccuucag caccacccug aagggcgccc     1860

agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc ggcguguuca     1920

acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc cuguucggcg     1980

gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg     2040

ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6350
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 2 opt

<400>  6350
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cggcuacgag accgacgagg     1080

acagagccaa gguggaggug acccccaaca gccccagagc cgaggccacc cugggcggcu     1140

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu     1200

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1380

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1440

gacuguucag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1500

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aagauccccg     1620

ugcagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1680

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1740

gcgacagcua caucgugauc ggcgugggcg acaagaagau cacccaccac uggcacagaa     1800

gcggcagcac ccugggcaag gccuucagca ccacccugaa gggcgcccag agacuggccg     1860

cccugggcga caccgccugg gacuucggca gcaucggcgg cguguucaac agcaucggca     1920

gagccgugca ccagguguuc ggcggcgccu ucagaacccu guucggcggc augagcugga     1980

ucacccaggg ccugaugggc gcccugcugc uguggauggg cgugaacgcc agagacagaa     2040

gcaucgcccu ggccuuccug gccaccggcg gcgugcuggu guuccuggcc accaacgugc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6351
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6351
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc acccugggca aggccuucag caccacccug aagggcgccc     1860

agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc ggcguguuca     1920

acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc cuguucggcg     1980

gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg     2040

ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6352
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  6352
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6353
<211>  2521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6353
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgccag aaaggagaag aagagaagag gcgccgacac cagcgugggc aucgugggcc      120

ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc uacuacaugu      180

accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug ggcaugaaca      240

agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug agcuacgagu      300

gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc aacaccacca      360

gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga agaagcagaa      420

gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc cagaccuggc      480

uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc uucagaaacc      540

ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc accagccaga      600

aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc agaugcaucg      660

gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug gacaucgugc      720

uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug gacaucgagc      780

uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac gaggccagca      840

ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac cuggacaagc      900

agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg ggcaacggcu      960

gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc agcaagaaga     1020

ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug agcgugcacg     1080

gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac gagaacagag     1140

ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc ggcuucggca     1200

gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug uacuaccuga     1260

ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccccugcccu     1320

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cugguggagu     1380

ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag gagggcgccg     1440

ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag ggcagacuga     1500

gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag ggcgugagcu     1560

acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc cugcacggca     1620

ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug cccgcccaga     1680

uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc aaccccguga     1740

ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc uucggcgaca     1800

gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac agaagcggca     1860

gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug gccgugcugg     1920

gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug ggcaagggca     1980

uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc ugguucagcc     2040

agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac ggcagcauca     2100

gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg     2160

acgugggcug cagcguggac uucagcaagu gaggacuagu gcaucacauu uaaaagcauc     2220

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2280

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2340

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2460

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2520

u                                                                     2521


<210>  6354
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6354
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc gugggccugc      120

ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac uacauguacc      180

uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc augaacaagu      240

gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc uacgagugcc      300

ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac accaccagca      360

ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga agcagaagag      420

ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag accuggcugg      480

agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc agaaaccccg      540

gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc agccagaagg      600

ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga ugcaucggcg      660

ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac aucgugcugg      720

agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac aucgagcugg      780

ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag gccagcauca      840

gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug gacaagcaga      900

gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc aacggcugcg      960

gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc aagaagauga     1020

ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc gugcacggca     1080

gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag aacagagcca     1140

agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc uucggcagcc     1200

ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac uaccugacca     1260

ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc cugcccuggc     1320

acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug guggaguuca     1380

aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag ggcgccgugc     1440

acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc agacugagca     1500

gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc gugagcuaca     1560

gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug cacggcaccg     1620

ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc gcccagaugg     1680

ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac cccgugauca     1740

ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc ggcgacagcu     1800

acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga agcggcagca     1860

ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc gugcugggcg     1920

acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc aagggcaucc     1980

accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg uucagccaga     2040

uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc agcaucagcc     2100

ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug agcgccgacg     2160

ugggcugcag cguggacuuc agcaagugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  6355
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6355
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaagaagaga agaggcgccg acaccagcgu gggcaucgug ggccugcugc      120

ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacgugg     2160

gcugcagcgu ggacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6356
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6356
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc cugcugcuga      120

ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug uaccuggaca      180

gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac aagugcuaca      240

uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag ugccccaugc      300

uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc agcaccuggg      360

ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga agagccguga      420

cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg cuggagagca      480

gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac cccggcuucg      540

cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag aaggugaucu      600

accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc ggcgugagca      660

acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug cuggagcacg      720

gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag cuggugacca      780

ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc aucagcgaca      840

uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag cagagcgaca      900

cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc ugcggccugu      960

ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag augaccggca     1020

agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac ggcagccagc     1080

acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga gccaaggugg     1140

agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug agcagcggcc     1500

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag auggccgugg     1680

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc agccugaugu     2100

gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc gacgugggcu     2160

gcagcgugga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6357
<211>  2509
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6357
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaagaa gagaagaggc gccgacacca gcgugggcau cgugggccug cugcugacca      120

ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac cuggacagaa      180

acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag ugcuacaucc      240

agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc cccaugcugg      300

acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc accugggugg      360

uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga gccgugaccc      420

ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug gagagcagag      480

aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc ggcuucgccc      540

uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag gugaucuacc      600

uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc gugagcaaca      660

gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug gagcacggcg      720

gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug gugaccacca      780

ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc agcgacaugg      840

ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag agcgacaccc      900

aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc ggccuguucg      960

gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug accggcaaga     1020

gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc agccagcaca     1080

gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc aagguggaga     1140

ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc cugggccugg     1200

acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc augaacaaca     1260

agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg cacgccggcg     1320

ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc aaggacgccc     1380

acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug cacaccgccc     1440

uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc agcggccacc     1500

ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac agccugugca     1560

ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc gugaccgugg     1620

aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug gccguggaca     1680

ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc accgagagca     1740

ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc uacaucguga     1800

ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc accaucggca     1860

aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc gacaccgccu     1920

gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc caccagaucu     1980

ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag auccugaucg     2040

gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc cugaugugcc     2100

uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac gugggcugca     2160

gcguggacuu cagcaaguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2220

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2280

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2340

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2460

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2509


<210>  6358
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6358
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug cugaccaccg      120

ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug gacagaaacg      180

acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc uacauccaga      240

ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc augcuggacg      300

agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc uggguggugu      360

acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc gugacccugc      420

ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag agcagagagu      480

acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc uucgcccugg      540

ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug aucuaccugg      600

ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug agcaacagag      660

acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag cacggcggcu      720

gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug accaccaccg      780

ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc gacauggcca      840

gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc gacacccagu      900

acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc cuguucggca      960

agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc ggcaagagca     1020

uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc cagcacagcg     1080

gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag guggagauca     1140

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu     1200

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1440

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga     1500

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1560

ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug accguggagg     1620

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1740

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1800

gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1860

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1920

acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     2040

cccugcugau guggcugggc cugaacacca agaacggcag caucagccug augugccugg     2100

cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacgug ggcugcagcg     2160

uggacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6359
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6359
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagagaag aggcgccgac accagcgugg gcaucguggg ccugcugcug accaccgcca      120

uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac agaaacgacg      180

ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac auccagauca      240

uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug cuggacgagg      300

gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg gugguguacg      360

gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug acccugccca      420

gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc agagaguaca      480

ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc gcccuggccg      540

ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc uaccugguga      600

ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc aacagagacu      660

ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg      720

ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc accaccguga      780

gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac auggccagcg      840

acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac acccaguacg      900

ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug uucggcaagg      960

gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc aagagcaucc     1020

agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag cacagcggca     1080

ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug gagaucaccc     1140

ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc cuggacugcg     1200

agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac aacaagcacu     1260

ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca     1320

ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac gcccacgcca     1380

agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc gcccuggccg     1440

gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc caccugaagu     1500

gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug ugcaccgccg     1560

ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc guggaggugc     1620

aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug gacaugcaga     1680

cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag agcaccgaga     1740

acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc gugaucggcg     1800

ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc ggcaaggccu     1860

ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc gccugggacu     1920

ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag aucuucggcg     1980

ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug aucggcaccc     2040

ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug ugccuggccc     2100

ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc ugcagcgugg     2160

acuucagcaa gugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  6360
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6360
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agagaagagg cgccgacacc agcgugggca ucgugggccu gcugcugacc accgccaugg      120

ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1860

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1920

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     2040

ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc cuggcccugg     2100

gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cgugggcugc agcguggacu     2160

ucagcaagug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  6361
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6361
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gaagaggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc gccauggccg      120

ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag gccuucgagg     1860

ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg gacuucggca     1920

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2040

uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug gcccugggcg     2100

gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcagc guggacuuca     2160

gcaagugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6362
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6362
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

gaggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc auggccgccg      120

aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac gccggcgagg      180

ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc auggaccugg      240

gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag ggcguggagc      300

ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac ggcaccugcc      360

accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc agccacagca      420

ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac accaagcacc      480

ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc gccgccgcca      540

ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug augauccugc      600

ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac uucguggagg      660

gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc gugaccguga      720

uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug agcaacaugg      780

ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc gacagcagau      840

gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac gugugcaaga      900

gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag ggcagccugg      960

ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc cagcccgaga     1020

accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc augaucguga     1080

acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc cccaacagcc     1140

ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc gagcccagaa     1200

ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac uggcuggugc     1260

acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac accggcaccc     1320

cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc aagagacaga     1380

ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc ggcgcccugg     1440

aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag ugcagacuga     1500

agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc gccuucaccu     1560

ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug caguacgccg     1620

gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag acccugaccc     1680

ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag aacagcaaga     1740

ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc gugggcgaga     1800

agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc uucgaggcca     1860

ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac uucggcagcg     1920

ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc gccgccuuca     1980

agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc cugcugaugu     2040

ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc cugggcggcg     2100

ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug gacuucagca     2160

agugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6363
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6363
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug gccgccgagg      120

ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc ggcgaggcca      180

ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug gaccugggcc      240

acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc accugccacc      360

acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc cacagcacca      420

gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc aagcaccuga      480

ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg      540

ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug auccugcuga      600

ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc guggagggca      660

ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug accgugaugg      720

cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc aacauggccg      780

aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac agcagaugcc      840

ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug ugcaagagaa      900

cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc agccugguga      960

ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag cccgagaacc     1020

uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug aucgugaacg     1080

acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc aacagcccca     1140

gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag cccagaaccg     1200

gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag agacagaccg     1380

ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc gcccuggagg     1440

ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc agacugaaga     1500

uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag uacgccggca     1620

ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc cugacccccg     1680

ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac agcaagauga     1740

ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga     1800

agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc gaggccaccg     1860

ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg     1920

gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug cugauguggc     2040

ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug ggcggcgugc     2100

ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cagcguggac uucagcaagu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6364
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6364
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cgugggcauc gugggccugc ugcugaccac cgccauggcc gccgagguga      120

ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc gaggccauca      180

gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac cugggccaca      240

ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc ugccaccaca      360

agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac agcaccagaa      420

agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag caccugauca      480

gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu      540

ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc cugcugaucg      600

cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug gagggcauga      660

gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc gugauggccc      720

aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac auggccgagg      780

ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc agaugcccca      840

cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc aagagaaccc      900

ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc cuggugaccu      960

gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc gagaaccugg     1020

aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc gugaacgaca     1080

ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac agccccagag     1140

ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc agaaccggcc     1200

uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga cagaccgugg     1380

uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg     1440

agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga cugaagaugg     1500

acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc accuucacca     1560

agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac gccggcaccg     1620

acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug acccccgugg     1680

gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc aagaugaugc     1740

uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc gagaagaaga     1800

ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag gccaccguga     1860

gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc agcgugggcg     1920

gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug auguggcugg     2040

gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc ggcgugcuga     2100

ucuuccugag caccgccgug agcgccgacg ugggcugcag cguggacuuc agcaagugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6365
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6365
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc gaggugacca      120

gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag gccaucagcu      180

uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug ggccacaugu      240

gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag cccgacgacg      300

uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc caccacaaga      360

agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc accagaaagc      420

ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac cugaucagag      480

uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc      540

ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug cugaucgccc      600

ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag ggcaugagcg      660

gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug auggcccagg      720

acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug gccgagguga      780

gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga ugccccaccc      840

agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag agaacccugg      900

uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug gugaccugcg      960

ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag aaccuggagu     1020

acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug aacgacaccg     1080

gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc cccagagccg     1140

aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga accggccugg     1200

acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag accguggugg     1380

ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug gaggccgaga     1440

uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug aagauggaca     1500

agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc uucaccaaga     1560

uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg     1620

gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc cccgugggca     1680

gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag augaugcugg     1740

agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag aagaagauca     1800

cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc accgugagag     1860

gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc gugggcggcg     1920

cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug uggcugggcc     2040

ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc gugcugaucu     2100

uccugagcac cgccgugagc gccgacgugg gcugcagcgu ggacuucagc aagugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6366
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6366
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag gugaccagaa      120

gaggcagcgc cuacuacaug uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc      180

ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc cacaugugcg      240

acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc gacgacgugg      300

acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac cacaagaagg      360

gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc agaaagcugc      420

agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug aucagagugg      480

agaacuggau cuucagaaac cccggcuucg cccuggccgc cgccgccauc gccuggcugc      540

ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug aucgcccccg      600

ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc augagcggcg      660

gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug gcccaggaca      720

agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc gaggugagaa      780

gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc cccacccagg      840

gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga acccuggugg      900

acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug accugcgcca      960

aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac cuggaguaca     1020

gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac gacaccggcc     1080

acgagaccga cgagaacaga gccaaggugg agaucacccc caacagcccc agagccgagg     1140

ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc ggccuggacu     1200

ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac aaggaguggu     1260

uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc cacuggaaca     1320

acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc gugguggugc     1380

ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag gccgagaugg     1440

acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag auggacaagc     1500

ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc accgacggcc     1620

ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc gugggcagac     1680

ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug augcuggagc     1740

uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag aagaucaccc     1800

accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc gugagaggcg     1860

ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc     1920

ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag agccuguucg     1980

gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg cugggccuga     2040

acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug cugaucuucc     2100

ugagcaccgc cgugagcgcc gacgugggcu gcagcgugga cuucagcaag ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6367
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6367
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccgac gugggcugca gcguggacuu cagcaaguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6368
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6368
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgccgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccgac gugggcugca gcguggacuu cagcaaguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6369
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6369
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug gccgccgccg      120

ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc ggcgaggcca      180

ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug gaccugggcc      240

acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc accugccacc      360

acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc cacagcacca      420

gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc aagcaccuga      480

ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg      540

ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug auccugcuga      600

ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc guggagggca      660

ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug accgugaugg      720

cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc aacauggccg      780

aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac agcagaugcc      840

ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug ugcaagagaa      900

cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc agccugguga      960

ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag cccgagaacc     1020

uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug aucgugaacg     1080

acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc aacagcccca     1140

gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag cccagaaccg     1200

gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag agacagaccg     1380

ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc gcccuggagg     1440

ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc agacugaaga     1500

uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag uacgccggca     1620

ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc cugacccccg     1680

ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac agcaagauga     1740

ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga     1800

agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc gaggccaccg     1860

ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg     1920

gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug cugauguggc     2040

ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug ggcggcgugc     2100

ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cagcguggac uucagcaagu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6370
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6370
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcag cagaaagaga agaagccacg acgugcugac cgugcaguuc cugauccugg      120

gcaugcugcu gaugaccggc ggcgugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacgugg     2160

gcugcagcgu ggacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6371
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6371
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcag cagaaagaga agaagccacg acgugcugac cgugcaguuc cugauccugg      120

gcaugcugcu gaugaccggc gccgugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacgugg     2160

gcugcagcgu ggacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6372
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6372
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6373
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6373
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgccag aaaggagaag aagagaagag gcgccgacac cagcgugggc aucgugggcc      120

ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc uacuacaugu      180

accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug ggcaugaaca      240

agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug agcuacgagu      300

gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc aacaccacca      360

gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga agaagcagaa      420

gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc cagaccuggc      480

uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc uucagaaacc      540

ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc accagccaga      600

aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc agaugcaucg      660

gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug gacaucgugc      720

uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug gacaucgagc      780

uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac gaggccagca      840

ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac cuggacaagc      900

agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg ggcaacggcu      960

gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc agcaagaaga     1020

ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug agcgugcacg     1080

gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac gagaacagag     1140

ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc ggcuucggca     1200

gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug uacuaccuga     1260

ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccccugcccu     1320

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cugguggagu     1380

ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag gagggcgccg     1440

ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag ggcagacuga     1500

gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag ggcgugagcu     1560

acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc cugcacggca     1620

ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug cccgcccaga     1680

uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc aaccccguga     1740

ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc uucggcgaca     1800

gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac agaagcggca     1860

gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug gccgugcugg     1920

gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug ggcaagggca     1980

uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc ugguucagcc     2040

agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac ggcagcauca     2100

gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg     2160

acgugggcug cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  6374
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6374
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc gugggccugc      120

ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac uacauguacc      180

uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc augaacaagu      240

gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc uacgagugcc      300

ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac accaccagca      360

ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga agcagaagag      420

ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag accuggcugg      480

agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc agaaaccccg      540

gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc agccagaagg      600

ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga ugcaucggcg      660

ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac aucgugcugg      720

agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac aucgagcugg      780

ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag gccagcauca      840

gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug gacaagcaga      900

gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc aacggcugcg      960

gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc aagaagauga     1020

ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc gugcacggca     1080

gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag aacagagcca     1140

agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc uucggcagcc     1200

ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac uaccugacca     1260

ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc cugcccuggc     1320

acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug guggaguuca     1380

aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag ggcgccgugc     1440

acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc agacugagca     1500

gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc gugagcuaca     1560

gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug cacggcaccg     1620

ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc gcccagaugg     1680

ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac cccgugauca     1740

ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc ggcgacagcu     1800

acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga agcggcagca     1860

ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc gugcugggcg     1920

acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc aagggcaucc     1980

accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg uucagccaga     2040

uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc agcaucagcc     2100

ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug agcgccgacg     2160

ugggcugcug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  6375
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6375
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaagaagaga agaggcgccg acaccagcgu gggcaucgug ggccugcugc      120

ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacgugg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6376
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6376
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc cugcugcuga      120

ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug uaccuggaca      180

gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac aagugcuaca      240

uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag ugccccaugc      300

uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc agcaccuggg      360

ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga agagccguga      420

cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg cuggagagca      480

gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac cccggcuucg      540

cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag aaggugaucu      600

accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc ggcgugagca      660

acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug cuggagcacg      720

gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag cuggugacca      780

ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc aucagcgaca      840

uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag cagagcgaca      900

cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc ugcggccugu      960

ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag augaccggca     1020

agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac ggcagccagc     1080

acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga gccaaggugg     1140

agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug agcagcggcc     1500

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag auggccgugg     1680

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc agccugaugu     2100

gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc gacgugggcu     2160

gcugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6377
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6377
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaagaa gagaagaggc gccgacacca gcgugggcau cgugggccug cugcugacca      120

ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac cuggacagaa      180

acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag ugcuacaucc      240

agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc cccaugcugg      300

acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc accugggugg      360

uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga gccgugaccc      420

ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug gagagcagag      480

aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc ggcuucgccc      540

uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag gugaucuacc      600

uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc gugagcaaca      660

gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug gagcacggcg      720

gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug gugaccacca      780

ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc agcgacaugg      840

ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag agcgacaccc      900

aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc ggccuguucg      960

gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug accggcaaga     1020

gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc agccagcaca     1080

gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc aagguggaga     1140

ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc cugggccugg     1200

acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc augaacaaca     1260

agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg cacgccggcg     1320

ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc aaggacgccc     1380

acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug cacaccgccc     1440

uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc agcggccacc     1500

ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac agccugugca     1560

ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc gugaccgugg     1620

aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug gccguggaca     1680

ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc accgagagca     1740

ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc uacaucguga     1800

ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc accaucggca     1860

aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc gacaccgccu     1920

gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc caccagaucu     1980

ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag auccugaucg     2040

gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc cugaugugcc     2100

uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac gugggcugcu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6378
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6378
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug cugaccaccg      120

ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug gacagaaacg      180

acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc uacauccaga      240

ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc augcuggacg      300

agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc uggguggugu      360

acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc gugacccugc      420

ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag agcagagagu      480

acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc uucgcccugg      540

ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug aucuaccugg      600

ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug agcaacagag      660

acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag cacggcggcu      720

gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug accaccaccg      780

ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc gacauggcca      840

gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc gacacccagu      900

acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc cuguucggca      960

agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc ggcaagagca     1020

uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc cagcacagcg     1080

gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag guggagauca     1140

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu     1200

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1440

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga     1500

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1560

ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug accguggagg     1620

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1740

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1800

gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1860

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1920

acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     2040

cccugcugau guggcugggc cugaacacca agaacggcag caucagccug augugccugg     2100

cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacgug ggcugcugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6379
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6379
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagagaag aggcgccgac accagcgugg gcaucguggg ccugcugcug accaccgcca      120

uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac agaaacgacg      180

ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac auccagauca      240

uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug cuggacgagg      300

gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg gugguguacg      360

gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug acccugccca      420

gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc agagaguaca      480

ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc gcccuggccg      540

ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc uaccugguga      600

ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc aacagagacu      660

ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg      720

ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc accaccguga      780

gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac auggccagcg      840

acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac acccaguacg      900

ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug uucggcaagg      960

gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc aagagcaucc     1020

agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag cacagcggca     1080

ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug gagaucaccc     1140

ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc cuggacugcg     1200

agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac aacaagcacu     1260

ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca     1320

ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac gcccacgcca     1380

agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc gcccuggccg     1440

gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc caccugaagu     1500

gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug ugcaccgccg     1560

ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc guggaggugc     1620

aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug gacaugcaga     1680

cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag agcaccgaga     1740

acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc gugaucggcg     1800

ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc ggcaaggccu     1860

ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc gccugggacu     1920

ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag aucuucggcg     1980

ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug aucggcaccc     2040

ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug ugccuggccc     2100

ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc ugcugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6380
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6380
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agagaagagg cgccgacacc agcgugggca ucgugggccu gcugcugacc accgccaugg      120

ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1860

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1920

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     2040

ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc cuggcccugg     2100

gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cgugggcugc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6381
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6381
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gaagaggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc gccauggccg      120

ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag gccuucgagg     1860

ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg gacuucggca     1920

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2040

uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug gcccugggcg     2100

gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6382
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6382
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

gaggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc auggccgccg      120

aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac gccggcgagg      180

ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc auggaccugg      240

gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag ggcguggagc      300

ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac ggcaccugcc      360

accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc agccacagca      420

ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac accaagcacc      480

ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc gccgccgcca      540

ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug augauccugc      600

ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac uucguggagg      660

gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc gugaccguga      720

uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug agcaacaugg      780

ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc gacagcagau      840

gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac gugugcaaga      900

gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag ggcagccugg      960

ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc cagcccgaga     1020

accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc augaucguga     1080

acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc cccaacagcc     1140

ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc gagcccagaa     1200

ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac uggcuggugc     1260

acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac accggcaccc     1320

cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc aagagacaga     1380

ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc ggcgcccugg     1440

aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag ugcagacuga     1500

agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc gccuucaccu     1560

ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug caguacgccg     1620

gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag acccugaccc     1680

ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag aacagcaaga     1740

ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc gugggcgaga     1800

agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc uucgaggcca     1860

ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac uucggcagcg     1920

ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc gccgccuuca     1980

agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc cugcugaugu     2040

ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc cugggcggcg     2100

ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6383
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6383
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug gccgccgagg      120

ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc ggcgaggcca      180

ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug gaccugggcc      240

acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc accugccacc      360

acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc cacagcacca      420

gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc aagcaccuga      480

ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg      540

ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug auccugcuga      600

ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc guggagggca      660

ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug accgugaugg      720

cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc aacauggccg      780

aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac agcagaugcc      840

ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug ugcaagagaa      900

cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc agccugguga      960

ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag cccgagaacc     1020

uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug aucgugaacg     1080

acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc aacagcccca     1140

gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag cccagaaccg     1200

gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag agacagaccg     1380

ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc gcccuggagg     1440

ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc agacugaaga     1500

uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag uacgccggca     1620

ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc cugacccccg     1680

ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac agcaagauga     1740

ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga     1800

agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc gaggccaccg     1860

ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg     1920

gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug cugauguggc     2040

ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug ggcggcgugc     2100

ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6384
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6384
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cgugggcauc gugggccugc ugcugaccac cgccauggcc gccgagguga      120

ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc gaggccauca      180

gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac cugggccaca      240

ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc ugccaccaca      360

agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac agcaccagaa      420

agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag caccugauca      480

gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu      540

ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc cugcugaucg      600

cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug gagggcauga      660

gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc gugauggccc      720

aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac auggccgagg      780

ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc agaugcccca      840

cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc aagagaaccc      900

ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc cuggugaccu      960

gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc gagaaccugg     1020

aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc gugaacgaca     1080

ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac agccccagag     1140

ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc agaaccggcc     1200

uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga cagaccgugg     1380

uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg     1440

agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga cugaagaugg     1500

acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc accuucacca     1560

agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac gccggcaccg     1620

acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug acccccgugg     1680

gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc aagaugaugc     1740

uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc gagaagaaga     1800

ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag gccaccguga     1860

gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc agcgugggcg     1920

gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug auguggcugg     2040

gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc ggcgugcuga     2100

ucuuccugag caccgccgug agcgccgacg ugggcugcug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6385
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6385
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc gaggugacca      120

gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag gccaucagcu      180

uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug ggccacaugu      240

gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag cccgacgacg      300

uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc caccacaaga      360

agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc accagaaagc      420

ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac cugaucagag      480

uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc      540

ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug cugaucgccc      600

ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag ggcaugagcg      660

gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug auggcccagg      720

acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug gccgagguga      780

gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga ugccccaccc      840

agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag agaacccugg      900

uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug gugaccugcg      960

ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag aaccuggagu     1020

acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug aacgacaccg     1080

gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc cccagagccg     1140

aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga accggccugg     1200

acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag accguggugg     1380

ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug gaggccgaga     1440

uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug aagauggaca     1500

agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc uucaccaaga     1560

uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg     1620

gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc cccgugggca     1680

gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag augaugcugg     1740

agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag aagaagauca     1800

cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc accgugagag     1860

gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc gugggcggcg     1920

cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug uggcugggcc     2040

ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc gugcugaucu     2100

uccugagcac cgccgugagc gccgacgugg gcugcugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6386
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6386
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag gugaccagaa      120

gaggcagcgc cuacuacaug uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc      180

ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc cacaugugcg      240

acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc gacgacgugg      300

acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac cacaagaagg      360

gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc agaaagcugc      420

agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug aucagagugg      480

agaacuggau cuucagaaac cccggcuucg cccuggccgc cgccgccauc gccuggcugc      540

ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug aucgcccccg      600

ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc augagcggcg      660

gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug gcccaggaca      720

agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc gaggugagaa      780

gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc cccacccagg      840

gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga acccuggugg      900

acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug accugcgcca      960

aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac cuggaguaca     1020

gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac gacaccggcc     1080

acgagaccga cgagaacaga gccaaggugg agaucacccc caacagcccc agagccgagg     1140

ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc ggccuggacu     1200

ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac aaggaguggu     1260

uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc cacuggaaca     1320

acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc gugguggugc     1380

ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag gccgagaugg     1440

acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag auggacaagc     1500

ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc accgacggcc     1620

ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc gugggcagac     1680

ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug augcuggagc     1740

uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag aagaucaccc     1800

accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc gugagaggcg     1860

ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc     1920

ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag agccuguucg     1980

gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg cugggccuga     2040

acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug cugaucuucc     2100

ugagcaccgc cgugagcgcc gacgugggcu gcugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6387
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6387
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccgac gugggcugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6388
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6388
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgccgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccgac gugggcugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6389
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6389
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug gccgccgccg      120

ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc ggcgaggcca      180

ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug gaccugggcc      240

acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc accugccacc      360

acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc cacagcacca      420

gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc aagcaccuga      480

ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg      540

ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug auccugcuga      600

ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc guggagggca      660

ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug accgugaugg      720

cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc aacauggccg      780

aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac agcagaugcc      840

ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug ugcaagagaa      900

cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc agccugguga      960

ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag cccgagaacc     1020

uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug aucgugaacg     1080

acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc aacagcccca     1140

gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag cccagaaccg     1200

gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag agacagaccg     1380

ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc gcccuggagg     1440

ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc agacugaaga     1500

uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag uacgccggca     1620

ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc cugacccccg     1680

ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac agcaagauga     1740

ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga     1800

agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc gaggccaccg     1860

ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg     1920

gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug cugauguggc     2040

ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug ggcggcgugc     2100

ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6390
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6390
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcag cagaaagaga agaagccacg acgugcugac cgugcaguuc cugauccugg      120

gcaugcugcu gaugaccggc ggcgugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacgugg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6391
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6391
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcag cagaaagaga agaagccacg acgugcugac cgugcaguuc cugauccugg      120

gcaugcugcu gaugaccggc gccgugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacgugg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6392
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6392
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6393
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6393
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgccag aaaggagaag aagagaagag gcgccgacac cagcgugggc aucgugggcc      120

ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc uacuacaugu      180

accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug ggcaugaaca      240

agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug agcuacgagu      300

gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc aacaccacca      360

gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga agaagcagaa      420

gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc cagaccuggc      480

uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc uucagaaacc      540

ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc accagccaga      600

aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc agaugcaucg      660

gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug gacaucgugc      720

uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug gacaucgagc      780

uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac gaggccagca      840

ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac cuggacaagc      900

agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg ggcaacggcu      960

gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc agcaagaaga     1020

ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug agcgugcacg     1080

gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac gagaacagag     1140

ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc ggcuucggca     1200

gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug uacuaccuga     1260

ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccccugcccu     1320

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cugguggagu     1380

ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag gagggcgccg     1440

ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag ggcagacuga     1500

gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag ggcgugagcu     1560

acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc cugcacggca     1620

ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug cccgcccaga     1680

uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc aaccccguga     1740

ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc uucggcgaca     1800

gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac agaagcggca     1860

gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug gccgugcugg     1920

gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug ggcaagggca     1980

uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc ugguucagcc     2040

agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac ggcagcauca     2100

gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg     2160

acugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6394
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6394
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc gugggccugc      120

ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac uacauguacc      180

uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc augaacaagu      240

gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc uacgagugcc      300

ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac accaccagca      360

ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga agcagaagag      420

ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag accuggcugg      480

agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc agaaaccccg      540

gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc agccagaagg      600

ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga ugcaucggcg      660

ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac aucgugcugg      720

agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac aucgagcugg      780

ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag gccagcauca      840

gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug gacaagcaga      900

gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc aacggcugcg      960

gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc aagaagauga     1020

ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc gugcacggca     1080

gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag aacagagcca     1140

agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc uucggcagcc     1200

ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac uaccugacca     1260

ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc cugcccuggc     1320

acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug guggaguuca     1380

aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag ggcgccgugc     1440

acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc agacugagca     1500

gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc gugagcuaca     1560

gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug cacggcaccg     1620

ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc gcccagaugg     1680

ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac cccgugauca     1740

ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc ggcgacagcu     1800

acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga agcggcagca     1860

ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc gugcugggcg     1920

acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc aagggcaucc     1980

accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg uucagccaga     2040

uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc agcaucagcc     2100

ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug agcgccgacu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6395
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6395
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaagaagaga agaggcgccg acaccagcgu gggcaucgug ggccugcugc      120

ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6396
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6396
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc cugcugcuga      120

ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug uaccuggaca      180

gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac aagugcuaca      240

uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag ugccccaugc      300

uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc agcaccuggg      360

ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga agagccguga      420

cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg cuggagagca      480

gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac cccggcuucg      540

cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag aaggugaucu      600

accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc ggcgugagca      660

acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug cuggagcacg      720

gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag cuggugacca      780

ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc aucagcgaca      840

uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag cagagcgaca      900

cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc ugcggccugu      960

ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag augaccggca     1020

agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac ggcagccagc     1080

acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga gccaaggugg     1140

agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug agcagcggcc     1500

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag auggccgugg     1680

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc agccugaugu     2100

gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc gacugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6397
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6397
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaagaa gagaagaggc gccgacacca gcgugggcau cgugggccug cugcugacca      120

ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac cuggacagaa      180

acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag ugcuacaucc      240

agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc cccaugcugg      300

acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc accugggugg      360

uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga gccgugaccc      420

ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug gagagcagag      480

aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc ggcuucgccc      540

uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag gugaucuacc      600

uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc gugagcaaca      660

gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug gagcacggcg      720

gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug gugaccacca      780

ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc agcgacaugg      840

ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag agcgacaccc      900

aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc ggccuguucg      960

gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug accggcaaga     1020

gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc agccagcaca     1080

gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc aagguggaga     1140

ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc cugggccugg     1200

acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc augaacaaca     1260

agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg cacgccggcg     1320

ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc aaggacgccc     1380

acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug cacaccgccc     1440

uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc agcggccacc     1500

ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac agccugugca     1560

ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc gugaccgugg     1620

aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug gccguggaca     1680

ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc accgagagca     1740

ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc uacaucguga     1800

ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc accaucggca     1860

aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc gacaccgccu     1920

gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc caccagaucu     1980

ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag auccugaucg     2040

gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc cugaugugcc     2100

uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6398
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6398
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug cugaccaccg      120

ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug gacagaaacg      180

acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc uacauccaga      240

ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc augcuggacg      300

agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc uggguggugu      360

acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc gugacccugc      420

ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag agcagagagu      480

acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc uucgcccugg      540

ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug aucuaccugg      600

ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug agcaacagag      660

acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag cacggcggcu      720

gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug accaccaccg      780

ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc gacauggcca      840

gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc gacacccagu      900

acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc cuguucggca      960

agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc ggcaagagca     1020

uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc cagcacagcg     1080

gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag guggagauca     1140

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu     1200

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1440

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga     1500

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1560

ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug accguggagg     1620

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1740

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1800

gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1860

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1920

acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     2040

cccugcugau guggcugggc cugaacacca agaacggcag caucagccug augugccugg     2100

cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6399
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6399
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagagaag aggcgccgac accagcgugg gcaucguggg ccugcugcug accaccgcca      120

uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac agaaacgacg      180

ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac auccagauca      240

uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug cuggacgagg      300

gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg gugguguacg      360

gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug acccugccca      420

gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc agagaguaca      480

ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc gcccuggccg      540

ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc uaccugguga      600

ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc aacagagacu      660

ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg      720

ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc accaccguga      780

gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac auggccagcg      840

acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac acccaguacg      900

ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug uucggcaagg      960

gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc aagagcaucc     1020

agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag cacagcggca     1080

ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug gagaucaccc     1140

ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc cuggacugcg     1200

agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac aacaagcacu     1260

ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca     1320

ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac gcccacgcca     1380

agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc gcccuggccg     1440

gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc caccugaagu     1500

gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug ugcaccgccg     1560

ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc guggaggugc     1620

aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug gacaugcaga     1680

cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag agcaccgaga     1740

acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc gugaucggcg     1800

ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc ggcaaggccu     1860

ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc gccugggacu     1920

ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag aucuucggcg     1980

ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug aucggcaccc     2040

ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug ugccuggccc     2100

ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6400
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6400
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agagaagagg cgccgacacc agcgugggca ucgugggccu gcugcugacc accgccaugg      120

ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1860

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1920

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     2040

ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc cuggcccugg     2100

gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6401
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6401
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gaagaggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc gccauggccg      120

ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag gccuucgagg     1860

ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg gacuucggca     1920

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2040

uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug gcccugggcg     2100

gcgugcugau cuuccugagc accgccguga gcgccgacug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6402
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6402
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

gaggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc auggccgccg      120

aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac gccggcgagg      180

ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc auggaccugg      240

gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag ggcguggagc      300

ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac ggcaccugcc      360

accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc agccacagca      420

ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac accaagcacc      480

ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc gccgccgcca      540

ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug augauccugc      600

ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac uucguggagg      660

gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc gugaccguga      720

uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug agcaacaugg      780

ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc gacagcagau      840

gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac gugugcaaga      900

gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag ggcagccugg      960

ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc cagcccgaga     1020

accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc augaucguga     1080

acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc cccaacagcc     1140

ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc gagcccagaa     1200

ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac uggcuggugc     1260

acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac accggcaccc     1320

cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc aagagacaga     1380

ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc ggcgcccugg     1440

aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag ugcagacuga     1500

agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc gccuucaccu     1560

ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug caguacgccg     1620

gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag acccugaccc     1680

ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag aacagcaaga     1740

ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc gugggcgaga     1800

agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc uucgaggcca     1860

ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac uucggcagcg     1920

ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc gccgccuuca     1980

agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc cugcugaugu     2040

ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc cugggcggcg     2100

ugcugaucuu ccugagcacc gccgugagcg ccgacugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6403
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6403
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug gccgccgagg      120

ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc ggcgaggcca      180

ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug gaccugggcc      240

acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc accugccacc      360

acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc cacagcacca      420

gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc aagcaccuga      480

ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg      540

ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug auccugcuga      600

ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc guggagggca      660

ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug accgugaugg      720

cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc aacauggccg      780

aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac agcagaugcc      840

ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug ugcaagagaa      900

cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc agccugguga      960

ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag cccgagaacc     1020

uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug aucgugaacg     1080

acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc aacagcccca     1140

gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag cccagaaccg     1200

gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag agacagaccg     1380

ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc gcccuggagg     1440

ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc agacugaaga     1500

uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag uacgccggca     1620

ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc cugacccccg     1680

ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac agcaagauga     1740

ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga     1800

agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc gaggccaccg     1860

ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg     1920

gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug cugauguggc     2040

ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug ggcggcgugc     2100

ugaucuuccu gagcaccgcc gugagcgccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6404
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6404
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cgugggcauc gugggccugc ugcugaccac cgccauggcc gccgagguga      120

ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc gaggccauca      180

gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac cugggccaca      240

ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc ugccaccaca      360

agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac agcaccagaa      420

agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag caccugauca      480

gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu      540

ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc cugcugaucg      600

cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug gagggcauga      660

gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc gugauggccc      720

aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac auggccgagg      780

ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc agaugcccca      840

cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc aagagaaccc      900

ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc cuggugaccu      960

gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc gagaaccugg     1020

aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc gugaacgaca     1080

ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac agccccagag     1140

ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc agaaccggcc     1200

uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga cagaccgugg     1380

uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg     1440

agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga cugaagaugg     1500

acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc accuucacca     1560

agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac gccggcaccg     1620

acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug acccccgugg     1680

gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc aagaugaugc     1740

uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc gagaagaaga     1800

ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag gccaccguga     1860

gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc agcgugggcg     1920

gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug auguggcugg     2040

gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc ggcgugcuga     2100

ucuuccugag caccgccgug agcgccgacu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6405
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6405
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc gaggugacca      120

gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag gccaucagcu      180

uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug ggccacaugu      240

gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag cccgacgacg      300

uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc caccacaaga      360

agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc accagaaagc      420

ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac cugaucagag      480

uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc      540

ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug cugaucgccc      600

ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag ggcaugagcg      660

gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug auggcccagg      720

acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug gccgagguga      780

gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga ugccccaccc      840

agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag agaacccugg      900

uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug gugaccugcg      960

ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag aaccuggagu     1020

acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug aacgacaccg     1080

gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc cccagagccg     1140

aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga accggccugg     1200

acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag accguggugg     1380

ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug gaggccgaga     1440

uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug aagauggaca     1500

agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc uucaccaaga     1560

uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg     1620

gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc cccgugggca     1680

gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag augaugcugg     1740

agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag aagaagauca     1800

cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc accgugagag     1860

gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc gugggcggcg     1920

cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug uggcugggcc     2040

ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc gugcugaucu     2100

uccugagcac cgccgugagc gccgacugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6406
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6406
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag gugaccagaa      120

gaggcagcgc cuacuacaug uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc      180

ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc cacaugugcg      240

acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc gacgacgugg      300

acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac cacaagaagg      360

gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc agaaagcugc      420

agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug aucagagugg      480

agaacuggau cuucagaaac cccggcuucg cccuggccgc cgccgccauc gccuggcugc      540

ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug aucgcccccg      600

ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc augagcggcg      660

gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug gcccaggaca      720

agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc gaggugagaa      780

gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc cccacccagg      840

gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga acccuggugg      900

acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug accugcgcca      960

aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac cuggaguaca     1020

gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac gacaccggcc     1080

acgagaccga cgagaacaga gccaaggugg agaucacccc caacagcccc agagccgagg     1140

ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc ggccuggacu     1200

ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac aaggaguggu     1260

uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc cacuggaaca     1320

acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc gugguggugc     1380

ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag gccgagaugg     1440

acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag auggacaagc     1500

ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc accgacggcc     1620

ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc gugggcagac     1680

ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug augcuggagc     1740

uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag aagaucaccc     1800

accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc gugagaggcg     1860

ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc     1920

ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag agccuguucg     1980

gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg cugggccuga     2040

acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug cugaucuucc     2100

ugagcaccgc cgugagcgcc gacugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  6407
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6407
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccgac ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6408
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6408
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgccgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccgac ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6409
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6409
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug gccgccgccg      120

ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc ggcgaggcca      180

ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug gaccugggcc      240

acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc accugccacc      360

acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc cacagcacca      420

gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc aagcaccuga      480

ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg      540

ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug auccugcuga      600

ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc guggagggca      660

ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug accgugaugg      720

cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc aacauggccg      780

aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac agcagaugcc      840

ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug ugcaagagaa      900

cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc agccugguga      960

ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag cccgagaacc     1020

uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug aucgugaacg     1080

acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc aacagcccca     1140

gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag cccagaaccg     1200

gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag agacagaccg     1380

ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc gcccuggagg     1440

ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc agacugaaga     1500

uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag uacgccggca     1620

ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc cugacccccg     1680

ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac agcaagauga     1740

ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga     1800

agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc gaggccaccg     1860

ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg     1920

gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug cugauguggc     2040

ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug ggcggcgugc     2100

ugaucuuccu gagcaccgcc gugagcgccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6410
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6410
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcag cagaaagaga agaagccacg acgugcugac cgugcaguuc cugauccugg      120

gcaugcugcu gaugaccggc ggcgugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6411
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6411
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcag cagaaagaga agaagccacg acgugcugac cgugcaguuc cugauccugg      120

gcaugcugcu gaugaccggc gccgugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6412
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6412
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6413
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6413
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc gugggccugc      120

ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac uacauguacc      180

uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc augaacaagu      240

gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc uacgagugcc      300

ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac accaccagca      360

ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga agcagaagag      420

ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag accuggcugg      480

agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc agaaaccccg      540

gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc agccagaagg      600

ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga ugcaucggcg      660

ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac aucgugcugg      720

agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac aucgagcugg      780

ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag gccagcauca      840

gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug gacaagcaga      900

gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc aacggcugcg      960

gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc aagaagauga     1020

ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc gugcacggca     1080

gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag aacagagcca     1140

agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc uucggcagcc     1200

ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac uaccugacca     1260

ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc cugcccuggc     1320

acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug guggaguuca     1380

aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag ggcgccgugc     1440

acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc agacugagca     1500

gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc gugagcuaca     1560

gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug cacggcaccg     1620

ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc gcccagaugg     1680

ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac cccgugauca     1740

ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc ggcgacagcu     1800

acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga agcggcagca     1860

ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc gugcugggcg     1920

acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc aagggcaucc     1980

accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg uucagccaga     2040

uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc agcaucagcc     2100

ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug agcgccugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6414
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6414
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgccag aaaggagaag aagagaagag gcgccgacac cagcgugggc aucgugggcc      120

ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc uacuacaugu      180

accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug ggcaugaaca      240

agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug agcuacgagu      300

gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc aacaccacca      360

gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga agaagcagaa      420

gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc cagaccuggc      480

uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc uucagaaacc      540

ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc accagccaga      600

aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc agaugcaucg      660

gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug gacaucgugc      720

uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug gacaucgagc      780

uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac gaggccagca      840

ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac cuggacaagc      900

agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg ggcaacggcu      960

gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc agcaagaaga     1020

ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug agcgugcacg     1080

gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac gagaacagag     1140

ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc ggcuucggca     1200

gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug uacuaccuga     1260

ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccccugcccu     1320

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cugguggagu     1380

ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag gagggcgccg     1440

ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag ggcagacuga     1500

gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag ggcgugagcu     1560

acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc cugcacggca     1620

ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug cccgcccaga     1680

uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc aaccccguga     1740

ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc uucggcgaca     1800

gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac agaagcggca     1860

gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug gccgugcugg     1920

gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug ggcaagggca     1980

uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc ugguucagcc     2040

agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac ggcagcauca     2100

gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6415
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6415
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaagaagaga agaggcgccg acaccagcgu gggcaucgug ggccugcugc      120

ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6416
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6416
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc cugcugcuga      120

ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug uaccuggaca      180

gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac aagugcuaca      240

uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag ugccccaugc      300

uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc agcaccuggg      360

ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga agagccguga      420

cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg cuggagagca      480

gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac cccggcuucg      540

cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag aaggugaucu      600

accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc ggcgugagca      660

acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug cuggagcacg      720

gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag cuggugacca      780

ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc aucagcgaca      840

uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag cagagcgaca      900

cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc ugcggccugu      960

ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag augaccggca     1020

agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac ggcagccagc     1080

acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga gccaaggugg     1140

agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug agcagcggcc     1500

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag auggccgugg     1680

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc agccugaugu     2100

gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6417
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6417
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaagaa gagaagaggc gccgacacca gcgugggcau cgugggccug cugcugacca      120

ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac cuggacagaa      180

acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag ugcuacaucc      240

agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc cccaugcugg      300

acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc accugggugg      360

uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga gccgugaccc      420

ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug gagagcagag      480

aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc ggcuucgccc      540

uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag gugaucuacc      600

uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc gugagcaaca      660

gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug gagcacggcg      720

gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug gugaccacca      780

ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc agcgacaugg      840

ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag agcgacaccc      900

aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc ggccuguucg      960

gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug accggcaaga     1020

gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc agccagcaca     1080

gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc aagguggaga     1140

ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc cugggccugg     1200

acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc augaacaaca     1260

agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg cacgccggcg     1320

ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc aaggacgccc     1380

acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug cacaccgccc     1440

uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc agcggccacc     1500

ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac agccugugca     1560

ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc gugaccgugg     1620

aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug gccguggaca     1680

ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc accgagagca     1740

ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc uacaucguga     1800

ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc accaucggca     1860

aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc gacaccgccu     1920

gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc caccagaucu     1980

ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag auccugaucg     2040

gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc cugaugugcc     2100

uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6418
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6418
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaagaagag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug cugaccaccg      120

ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug gacagaaacg      180

acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc uacauccaga      240

ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc augcuggacg      300

agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc uggguggugu      360

acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc gugacccugc      420

ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag agcagagagu      480

acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc uucgcccugg      540

ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug aucuaccugg      600

ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug agcaacagag      660

acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag cacggcggcu      720

gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug accaccaccg      780

ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc gacauggcca      840

gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc gacacccagu      900

acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc cuguucggca      960

agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc ggcaagagca     1020

uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc cagcacagcg     1080

gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag guggagauca     1140

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu     1200

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1440

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga     1500

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1560

ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug accguggagg     1620

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1740

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1800

gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1860

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1920

acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     2040

cccugcugau guggcugggc cugaacacca agaacggcag caucagccug augugccugg     2100

cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6419
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6419
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaagagaag aggcgccgac accagcgugg gcaucguggg ccugcugcug accaccgcca      120

uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac agaaacgacg      180

ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac auccagauca      240

uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug cuggacgagg      300

gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg gugguguacg      360

gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug acccugccca      420

gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc agagaguaca      480

ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc gcccuggccg      540

ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc uaccugguga      600

ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc aacagagacu      660

ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg      720

ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc accaccguga      780

gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac auggccagcg      840

acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac acccaguacg      900

ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug uucggcaagg      960

gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc aagagcaucc     1020

agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag cacagcggca     1080

ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug gagaucaccc     1140

ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc cuggacugcg     1200

agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac aacaagcacu     1260

ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca     1320

ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac gcccacgcca     1380

agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc gcccuggccg     1440

gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc caccugaagu     1500

gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug ugcaccgccg     1560

ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc guggaggugc     1620

aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug gacaugcaga     1680

cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag agcaccgaga     1740

acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc gugaucggcg     1800

ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc ggcaaggccu     1860

ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc gccugggacu     1920

ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag aucuucggcg     1980

ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug aucggcaccc     2040

ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug ugccuggccc     2100

ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6420
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6420
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

agagaagagg cgccgacacc agcgugggca ucgugggccu gcugcugacc accgccaugg      120

ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1860

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1920

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     2040

ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc cuggcccugg     2100

gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6421
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6421
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gaagaggcgc cgacaccagc gugggcaucg ugggccugcu gcugaccacc gccauggccg      120

ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag gccuucgagg     1860

ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg gacuucggca     1920

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2040

uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug gcccugggcg     2100

gcgugcugau cuuccugagc accgccguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6422
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6422
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

gaggcgccga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc auggccgccg      120

aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac gccggcgagg      180

ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc auggaccugg      240

gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag ggcguggagc      300

ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac ggcaccugcc      360

accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc agccacagca      420

ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac accaagcacc      480

ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc gccgccgcca      540

ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug augauccugc      600

ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac uucguggagg      660

gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc gugaccguga      720

uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug agcaacaugg      780

ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc gacagcagau      840

gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac gugugcaaga      900

gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag ggcagccugg      960

ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc cagcccgaga     1020

accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc augaucguga     1080

acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc cccaacagcc     1140

ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc gagcccagaa     1200

ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac uggcuggugc     1260

acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac accggcaccc     1320

cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc aagagacaga     1380

ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc ggcgcccugg     1440

aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag ugcagacuga     1500

agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc gccuucaccu     1560

ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug caguacgccg     1620

gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag acccugaccc     1680

ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag aacagcaaga     1740

ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc gugggcgaga     1800

agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc uucgaggcca     1860

ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac uucggcagcg     1920

ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc gccgccuuca     1980

agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc cugcugaugu     2040

ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc cugggcggcg     2100

ugcugaucuu ccugagcacc gccgugagcg ccugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6423
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6423
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug gccgccgagg      120

ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc ggcgaggcca      180

ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug gaccugggcc      240

acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc accugccacc      360

acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc cacagcacca      420

gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc aagcaccuga      480

ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg      540

ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug auccugcuga      600

ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc guggagggca      660

ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug accgugaugg      720

cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc aacauggccg      780

aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac agcagaugcc      840

ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug ugcaagagaa      900

cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc agccugguga      960

ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag cccgagaacc     1020

uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug aucgugaacg     1080

acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc aacagcccca     1140

gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag cccagaaccg     1200

gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag agacagaccg     1380

ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc gcccuggagg     1440

ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc agacugaaga     1500

uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag uacgccggca     1620

ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc cugacccccg     1680

ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac agcaagauga     1740

ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga     1800

agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc gaggccaccg     1860

ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg     1920

gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug cugauguggc     2040

ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug ggcggcgugc     2100

ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6424
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6424
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cgugggcauc gugggccugc ugcugaccac cgccauggcc gccgagguga      120

ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc gaggccauca      180

gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac cugggccaca      240

ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc ugccaccaca      360

agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac agcaccagaa      420

agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag caccugauca      480

gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu      540

ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc cugcugaucg      600

cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug gagggcauga      660

gcggcggcac cuggguggac aucgugcugg agcacggcgg cugcgugacc gugauggccc      720

aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac auggccgagg      780

ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc agaugcccca      840

cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc aagagaaccc      900

ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc cuggugaccu      960

gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc gagaaccugg     1020

aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc gugaacgaca     1080

ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac agccccagag     1140

ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc agaaccggcc     1200

uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga cagaccgugg     1380

uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg     1440

agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga cugaagaugg     1500

acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc accuucacca     1560

agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac gccggcaccg     1620

acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug acccccgugg     1680

gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc aagaugaugc     1740

uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc gagaagaaga     1800

ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag gccaccguga     1860

gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc agcgugggcg     1920

gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug auguggcugg     2040

gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc ggcgugcuga     2100

ucuuccugag caccgccgug agcgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6425
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6425
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucgug ggccugcugc ugaccaccgc cauggccgcc gaggugacca      120

gaagaggcag cgccuacuac auguaccugg acagaaacga cgccggcgag gccaucagcu      180

uccccaccac ccugggcaug aacaagugcu acauccagau cauggaccug ggccacaugu      240

gcgacgccac caugagcuac gagugcccca ugcuggacga gggcguggag cccgacgacg      300

uggacugcug gugcaacacc accagcaccu ggguggugua cggcaccugc caccacaaga      360

agggcgaggc cagaagaagc agaagagccg ugacccugcc cagccacagc accagaaagc      420

ugcagaccag aagccagacc uggcuggaga gcagagagua caccaagcac cugaucagag      480

uggagaacug gaucuucaga aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc      540

ugcugggcag cagcaccagc cagaagguga ucuaccuggu gaugauccug cugaucgccc      600

ccgccuacag caucagaugc aucggcguga gcaacagaga cuucguggag ggcaugagcg      660

gcggcaccug gguggacauc gugcuggagc acggcggcug cgugaccgug auggcccagg      720

acaagcccac cguggacauc gagcugguga ccaccaccgu gagcaacaug gccgagguga      780

gaagcuacug cuacgaggcc agcaucagcg acauggccag cgacagcaga ugccccaccc      840

agggcgaggc cuaccuggac aagcagagcg acacccagua cgugugcaag agaacccugg      900

uggacagagg cuggggcaac ggcugcggcc uguucggcaa gggcagccug gugaccugcg      960

ccaaguucgc cugcagcaag aagaugaccg gcaagagcau ccagcccgag aaccuggagu     1020

acagaaucau gcugagcgug cacggcagcc agcacagcgg caugaucgug aacgacaccg     1080

gccacgagac cgacgagaac agagccaagg uggagaucac ccccaacagc cccagagccg     1140

aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga accggccugg     1200

acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag accguggugg     1380

ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug gaggccgaga     1440

uggacggcgc caagggcaga cugagcagcg gccaccugaa gugcagacug aagauggaca     1500

agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc uucaccaaga     1560

uccccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg     1620

gccccugcaa ggugcccgcc cagauggccg uggacaugca gacccugacc cccgugggca     1680

gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag augaugcugg     1740

agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgag aagaagauca     1800

cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc accgugagag     1860

gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc gugggcggcg     1920

cccugaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcugaug uggcugggcc     2040

ugaacaccaa gaacggcagc aucagccuga ugugccuggc ccugggcggc gugcugaucu     2100

uccugagcac cgccgugagc gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  6426
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6426
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgugggc cugcugcuga ccaccgccau ggccgccgag gugaccagaa      120

gaggcagcgc cuacuacaug uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc      180

ccaccacccu gggcaugaac aagugcuaca uccagaucau ggaccugggc cacaugugcg      240

acgccaccau gagcuacgag ugccccaugc uggacgaggg cguggagccc gacgacgugg      300

acugcuggug caacaccacc agcaccuggg ugguguacgg caccugccac cacaagaagg      360

gcgaggccag aagaagcaga agagccguga cccugcccag ccacagcacc agaaagcugc      420

agaccagaag ccagaccugg cuggagagca gagaguacac caagcaccug aucagagugg      480

agaacuggau cuucagaaac cccggcuucg cccuggccgc cgccgccauc gccuggcugc      540

ugggcagcag caccagccag aaggugaucu accuggugau gauccugcug aucgcccccg      600

ccuacagcau cagaugcauc ggcgugagca acagagacuu cguggagggc augagcggcg      660

gcaccugggu ggacaucgug cuggagcacg gcggcugcgu gaccgugaug gcccaggaca      720

agcccaccgu ggacaucgag cuggugacca ccaccgugag caacauggcc gaggugagaa      780

gcuacugcua cgaggccagc aucagcgaca uggccagcga cagcagaugc cccacccagg      840

gcgaggccua ccuggacaag cagagcgaca cccaguacgu gugcaagaga acccuggugg      900

acagaggcug gggcaacggc ugcggccugu ucggcaaggg cagccuggug accugcgcca      960

aguucgccug cagcaagaag augaccggca agagcaucca gcccgagaac cuggaguaca     1020

gaaucaugcu gagcgugcac ggcagccagc acagcggcau gaucgugaac gacaccggcc     1080

acgagaccga cgagaacaga gccaaggugg agaucacccc caacagcccc agagccgagg     1140

ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc ggccuggacu     1200

ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac aaggaguggu     1260

uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc cacuggaaca     1320

acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc gugguggugc     1380

ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag gccgagaugg     1440

acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag auggacaagc     1500

ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc accaagaucc     1560

ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc accgacggcc     1620

ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc gugggcagac     1680

ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug augcuggagc     1740

uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgagaag aagaucaccc     1800

accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc gugagaggcg     1860

ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc     1920

ugaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag agccuguucg     1980

gcggcaugag cugguucagc cagauccuga ucggcacccu gcugaugugg cugggccuga     2040

acaccaagaa cggcagcauc agccugaugu gccuggcccu gggcggcgug cugaucuucc     2100

ugagcaccgc cgugagcgcc ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6427
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  6427
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6428
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6428
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgccgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6429
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6429
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggc aucgugggcc ugcugcugac caccgccaug gccgccgccg      120

ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc ggcgaggcca      180

ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug gaccugggcc      240

acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc guggagcccg      300

acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc accugccacc      360

acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc cacagcacca      420

gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc aagcaccuga      480

ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg      540

ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug auccugcuga      600

ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc guggagggca      660

ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug accgugaugg      720

cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc aacauggccg      780

aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac agcagaugcc      840

ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug ugcaagagaa      900

cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc agccugguga      960

ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag cccgagaacc     1020

uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug aucgugaacg     1080

acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc aacagcccca     1140

gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag cccagaaccg     1200

gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg cuggugcaca     1260

aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc ggcacccccc     1320

acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag agacagaccg     1380

ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc gcccuggagg     1440

ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc agacugaaga     1500

uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc uucaccuuca     1560

ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag uacgccggca     1620

ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc cugacccccg     1680

ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac agcaagauga     1740

ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga     1800

agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc gaggccaccg     1860

ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg     1920

gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc gccuucaaga     1980

gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug cugauguggc     2040

ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug ggcggcgugc     2100

ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6430
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6430
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcag cagaaagaga agaagccacg acgugcugac cgugcaguuc cugauccugg      120

gcaugcugcu gaugaccggc ggcgugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6431
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6431
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcag cagaaagaga agaagccacg acgugcugac cgugcaguuc cugauccugg      120

gcaugcugcu gaugaccggc gccgugacca gaagaggcag cgccuacuac auguaccugg      180

acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug aacaagugcu      240

acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac gagugcccca      300

ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc accagcaccu      360

ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc agaagagccg      420

ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc uggcuggaga      480

gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga aaccccggcu      540

ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc cagaagguga      600

ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc aucggcguga      660

gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc gugcuggagc      720

acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc gagcugguga      780

ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc agcaucagcg      840

acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac aagcagagcg      900

acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac ggcugcggcc      960

uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag aagaugaccg     1020

gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug cacggcagcc     1080

agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac agagccaagg     1140

uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg     1200

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga     1260

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1320

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1380

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1440

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cugagcagcg     1500

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1560

ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac ggcaccguga     1620

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc cagauggccg     1680

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1740

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1800

ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc ggcagcacca     1860

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1920

ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag ggcauccacc     1980

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     2040

ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     2100

ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6432
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 2 opt

<400>  6432
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6433
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 2 opt

<400>  6433
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaccug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6434
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 2 opt

<400>  6434
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggacaga gccaaggugg     1140

aggugacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug uucagcggcc     1500

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1680

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgugu ucaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcuggugugg cugggccuga acaccaagaa cggcagcauc agccugaccu     2100

gccuggcccu gggcggcgug augaucuucc ugagcaccgc cgugagcgcc gacgugggcu     2160

gcagcgugga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6435
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 2 opt

<400>  6435
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6436
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 2 opt

<400>  6436
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg cuacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcaagc     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag auccccgugc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6437
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 2 opt

<400>  6437
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cacccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacgagaa cagagccaag guggagguga     1140

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu     1200

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1440

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc ggccaccuga     1500

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1560

ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug accguggagg     1620

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1740

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1800

gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1860

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1920

acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     2040

cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug accugccugg     2100

cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug ggcugcagcg     2160

uggacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6438
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 2 opt

<400>  6438
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc acagaaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg cuacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcaagc     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag auccccgugc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6439
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 2 opt

<400>  6439
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaccguga acgacaucgg cuacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcaagc     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag auccccgugc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6440
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 2 opt

<400>  6440
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucggcu acgagaccga cgagaacaga gccaaggugg     1140

aggugacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc     1200

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca     1260

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1320

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1380

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1440

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcaagcug uucagcggcc     1500

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1560

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1620

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1680

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1740

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1800

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1860

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1920

ccugggacuu cggcagcgug ggcggcgugu ucaacagccu gggcaagggc auccaccaga     1980

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     2040

ucggcacccu gcuggugugg cugggccuga acaccaagaa cggcagcauc agccugaccu     2100

gccuggcccu gggcggcgug augaucuucc ugagcaccgc cgugagcgcc gacgugggcu     2160

gcagcgugga cuucagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6441
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 2 opt

<400>  6441
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcagag     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6442
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 2 opt

<400>  6442
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga cgccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6443
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 2 opt

<400>  6443
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggccu ggcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6444
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 2 opt

<400>  6444
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg uacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6445
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 2 opt

<400>  6445
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgagaucag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6446
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 2 opt

<400>  6446
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcuggccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6447
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 2 opt

<400>  6447
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccac ccuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6448
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 2 opt

<400>  6448
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc guggugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6449
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 2 opt

<400>  6449
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcgccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga ccccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6450
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 2 opt

<400>  6450
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagca      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6451
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 2 opt

<400>  6451
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagac      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6452
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 2 opt

<400>  6452
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaagcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacacccag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6453
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 2 opt

<400>  6453
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccaacgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccagaaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6454
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 2 opt

<400>  6454
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga gaccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6455
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 2 opt

<400>  6455
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc cugagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6456
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 2 opt

<400>  6456
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccaacgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg auguucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6457
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 2 opt

<400>  6457
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg auccugagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc aucggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6458
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 2 opt

<400>  6458
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugag caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

ccaucagcga caucgccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgccguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6459
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 2 opt

<400>  6459
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcacc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6460
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 2 opt

<400>  6460
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggagcug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6461
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 2 opt

<400>  6461
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga gaccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagaau cgagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6462
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 2 opt

<400>  6462
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaucgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6463
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 2 opt

<400>  6463
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgug aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagcuucau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6464
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 2 opt

<400>  6464
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcaagc     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6465
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 2 opt

<400>  6465
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga ggcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6466
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 2 opt

<400>  6466
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcgccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6467
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 2 opt

<400>  6467
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cggcaaggag aagaagagaa gaggcgccga caccaacgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccagaaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6468
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 2 opt

<400>  6468
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga gaccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaaggac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6469
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 2 opt

<400>  6469
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggugagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6470
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 2 opt

<400>  6470
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccaacgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacauga      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6471
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 2 opt

<400>  6471
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccgcccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagacccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6472
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 2 opt

<400>  6472
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga gaccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccu ggccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6473
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 2 opt

<400>  6473
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcaucag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcauccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6474
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 2 opt

<400>  6474
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaccug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ucagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6475
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 2 opt

<400>  6475
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc cagagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6476
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 2 opt

<400>  6476
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcaggccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6477
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 2 opt

<400>  6477
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6478
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 2 opt

<400>  6478
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcaccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacac cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gggcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu gcugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6479
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 2 opt

<400>  6479
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccgcca uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augcugguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggccc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgcc accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag accguggacg     1620

gcaccgugac cguggagggc caguacggcg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucaucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

gcggcguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6480
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 2 opt

<400>  6480
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcaccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucagcaagau ccccgccgag acccugcacg     1620

gcaccgugac cgaggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6481
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 2 opt

<400>  6481
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcaccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6482
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 2 opt

<400>  6482
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcaccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgugg aggugaccag aagaggcaac gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc augggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6483
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 2 opt

<400>  6483
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcaccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccuggu gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6484
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 2 opt

<400>  6484
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcaccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgugg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccgcc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6485
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 2 opt

<400>  6485
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgugg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc cugggcauga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccguga ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6486
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 2 opt

<400>  6486
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgugg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcgagg ccaucagcuu ccccaccacc cugggcguga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg uacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6487
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 2 opt

<400>  6487
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag aagaagagaa gaggcgccga caccagcgug ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgugg aggugaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu ccccaccacc cugggcguga      240

acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca     1140

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6488
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 2 opt

<400>  6488
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cacccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6489
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 2 opt

<400>  6489
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccaac cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcgugcacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6490
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 2 opt

<400>  6490
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6491
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 2 opt

<400>  6491
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccaac cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggcc gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacgagaa cagagccaag guggagguga     1140

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu     1200

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1440

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc ggccaccuga     1500

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1560

ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug accguggagg     1620

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1740

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1800

gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1860

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1920

acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     2040

cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug accugccugg     2100

cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug ggcugcagcg     2160

uggacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6492
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 2 opt

<400>  6492
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6493
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 2 opt

<400>  6493
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc agcacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gugugcaccg ccgccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6494
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 2 opt

<400>  6494
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga cgccagcauc ggcaucguga      120

gccugcugcu gaccaccgug auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgugaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacgagaa cagagccaag guggagguga     1140

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu     1200

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac agcggcgccg     1320

acaccgagac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1440

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga     1500

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1560

ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug accguggagg     1620

ugcaguacgc cggcagagac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1740

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1800

gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcauc aucggcaagg     1860

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1920

acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     2040

cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug accugccugg     2100

cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug ggcugcagcg     2160

uggacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6495
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 2 opt

<400>  6495
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucgugg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agaagaagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      540

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      600

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacgagaa cagagccaag guggagguga     1140

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu     1200

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc     1260

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1320

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1380

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1440

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc ggccaccuga     1500

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1560

ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug accguggagg     1620

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1680

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1740

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1800

gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1860

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1920

acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac cagaucuucg     1980

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     2040

cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug accugccugg     2100

cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccgacgug ggcugcagcg     2160

uggacuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6496
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 2 opt

<400>  6496
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc cugggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgagacc agaagaagca      420

gaagaagcgu gagccugaga uaccacuaca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac aagaagcacc ugaucauggu ggagaacugg aucuucagaa      540

accccggcuu cgccaucgug agcguggcca ucaccuggcu gaugggcagc cugaccagcc      600

agaaggugau cuaccuggug augaucgugc ugaucgugcc cgccuacagc aucagcugca      660

ucggcgugag caacagagac cugguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccgaga uggcccagga caagcccacc guggacaucg      780

agcuggugac caugaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gccugagcga cauggccagc gccagcagau gccccaccca gggcgagccc agccuggaca      900

agcagagcga cacccagagc gugugcaaga gaacccuggg cgacagaggc uggggcaacg      960

gcugcggcau cuucggcaag ggcagccugg ugaccugcag caaguucacc ugcugcaaga     1020

agaugcccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugcccgugc     1080

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug cagagcgccg gcaccgacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1740

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6497
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 2 opt

<400>  6497
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc cagaaaggag agaaagagaa gaggcgccga caccagcauc ggcaucaucg      120

gccugcugcu gaccaccgcc auggccgccg agaucaccag aagaggcagc gccuacuaca      180

uguaccugga cagaagcgac gccggcaagg ccaucagcuu cgccaccacc augggcguga      240

acaagugcca cgugcagauc auggaccugg gccacaugug cgacgccacc augagcuacg      300

agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg ugcaacacca      360

ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc agacccagca      420

gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      480

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg auccugagaa      540

accccggcuu caccguggcc gcccuggcca uggccuggcu gcugggcaac agcaccagcc      600

agagagugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      660

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      720

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      780

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      840

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      900

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      960

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga     1020

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc     1080

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca     1140

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg     1200

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc     1260

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1320

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1380

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1440

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1500

uguucagcgg ccaccugaag ugcagaauca agauggacaa gcugagacug aagggcguga     1560

gcuacagccu gugcaccgcc gccuucaccu ucagcaaggu gcccgccgag acccugcacg     1620

gcaccgugac cguggaggug caguacgccg gcagagacgg ccccugcaag gugcccgccc     1680

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaacccca     1740

ucaucaccga gagcaccgag aacagcagaa ugaugcugga gcuggacccc cccuucggcg     1800

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1860

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1920

ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc cugggcaagg     1980

gcgugcacca gguguucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     2040

gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag aacggcagca     2100

ucagccuggc cugccuggcc cugggcggcg ccaugaucuu ccugagcacc gccgugagcg     2160

ccgacguggg cugcagcgug gacuucugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  6498
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 2 opt

<400>  6498
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6499
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 2 opt

<400>  6499
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac accugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaacg     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6500
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 2 opt

<400>  6500
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cggcuacgag accgacgagg     1080

acagagccaa gguggaggug acccccaaca gccccagagc cgaggccacc cugggcggcu     1140

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu     1200

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1380

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1440

gacuguucag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1500

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aagauccccg     1620

ugcagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1680

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1740

gcgacagcua caucgugauc ggcgugggcg acaagaagau cacccaccac uggcacagaa     1800

gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag agaauggccg     1860

ugcugggcga caccgccugg gacuucggca gcgugggcgg cguguucaac agccugggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc augagcuggu     1980

ucagccagau ccugaucggc acccugcugg uguggcuggg ccugaacacc aagaacggca     2040

gcaucagccu gaccugccug gcccugggcg gcgugaugau cuuccugagc accgccguga     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6501
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 2 opt

<400>  6501
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6502
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 2 opt

<400>  6502
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggcuacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg caagcuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6503
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 2 opt

<400>  6503
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccacaga aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggcuacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg caagcuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6504
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 2 opt

<400>  6504
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugac cgugaacgac aucggcuacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg caagcuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6505
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 2 opt

<400>  6505
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cgugggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucaccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac gagaacagag     1080

ccaaggugga ggugaccccc aacagcccca gagccgaggc cacccugggc ggcuucggca     1140

gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug uacuaccuga     1200

ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccccugcccu     1260

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cugguggagu     1320

ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag gagggcgccg     1380

ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag ggcagacugu     1440

ucagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag ggcgugagcu     1500

acagccugug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc cugcacggca     1560

ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug cccgcccaga     1620

uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc aaccccguga     1680

ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc uucggcgaca     1740

gcuacaucgu gaucggcgug ggcgacaaga agaucaccca ccacuggcac agaagcggca     1800

gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug gccgugcugg     1860

gcgacaccgc cugggacuuc ggcagcgugg gcggcguguu caacagccug ggcaagggca     1920

uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc ugguucagcc     1980

agauccugau cggcacccug cugguguggc ugggccugaa caccaagaac ggcagcauca     2040

gccugaccug ccuggcccug ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6506
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 2 opt

<400>  6506
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cggcuacgag accgacgaga     1080

acagagccaa gguggaggug acccccaaca gccccagagc cgaggccacc cugggcggcu     1140

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu     1200

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1380

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1440

agcuguucag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1500

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aagauccccg     1620

ugcagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1680

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1740

gcgacagcua caucgugauc ggcgugggcg acaagaagau cacccaccac uggcacagaa     1800

gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag agaauggccg     1860

ugcugggcga caccgccugg gacuucggca gcgugggcgg cguguucaac agccugggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc augagcuggu     1980

ucagccagau ccugaucggc acccugcugg uguggcuggg ccugaacacc aagaacggca     2040

gcaucagccu gaccugccug gcccugggcg gcgugaugau cuuccugagc accgccguga     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6507
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 2 opt

<400>  6507
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg cagaggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6508
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 2 opt

<400>  6508
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggccuggccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6509
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 2 opt

<400>  6509
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acugguacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6510
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 2 opt

<400>  6510
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca cccagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6511
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 2 opt

<400>  6511
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag aucagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6512
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 2 opt

<400>  6512
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcu ggcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6513
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 2 opt

<400>  6513
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccacccugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6514
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 2 opt

<400>  6514
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccguggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6515
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 2 opt

<400>  6515
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc gccugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagacccca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6516
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 2 opt

<400>  6516
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagcaagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6517
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 2 opt

<400>  6517
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagacagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6518
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 2 opt

<400>  6518
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcacccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6519
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 2 opt

<400>  6519
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagccugag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6520
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 2 opt

<400>  6520
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaaucgaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6521
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 2 opt

<400>  6521
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggauccu gagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcaucgg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6522
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 2 opt

<400>  6522
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugagcacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccaccauc agcgacaucg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgcc guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6523
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 2 opt

<400>  6523
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg agcugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6524
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 2 opt

<400>  6524
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ucgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6525
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 2 opt

<400>  6525
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgugaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc uucaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6526
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 2 opt

<400>  6526
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg caagcugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6527
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 2 opt

<400>  6527
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa aggacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6528
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 2 opt

<400>  6528
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggaggccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6529
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 2 opt

<400>  6529
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggccg     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag acccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6530
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 2 opt

<400>  6530
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcgcc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6531
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 2 opt

<400>  6531
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccuggccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6532
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 2 opt

<400>  6532
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ugagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6533
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 2 opt

<400>  6533
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc aucagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcauc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6534
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 2 opt

<400>  6534
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac accugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaucaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaacg     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6535
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 2 opt

<400>  6535
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggcccagag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6536
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 2 opt

<400>  6536
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcagg      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6537
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 2 opt

<400>  6537
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucagc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgagg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6538
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 2 opt

<400>  6538
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacaccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagggca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgugcuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6539
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 2 opt

<400>  6539
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6540
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 2 opt

<400>  6540
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cguggaggug accagaagag      120

gcaacgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccaccauggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6541
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 2 opt

<400>  6541
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cuggugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6542
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 2 opt

<400>  6542
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugaacaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cuggugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6543
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 2 opt

<400>  6543
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cguggaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccgccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6544
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 2 opt

<400>  6544
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cguggaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgugaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6545
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 2 opt

<400>  6545
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cguggaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg cgaggccauc agcuucccca      180

ccacccuggg cgugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acugguacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6546
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 2 opt

<400>  6546
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cguggaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucccca      180

ccacccuggg cgugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac aucggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6547
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 2 opt

<400>  6547
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cgugggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucaccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6548
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 2 opt

<400>  6548
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccaaccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac aucggccacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcgug caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6549
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 2 opt

<400>  6549
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac aucggccacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6550
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 2 opt

<400>  6550
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccaaccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac gagaacagag     1080

ccaaggugga ggugaccccc aacagcccca gagccgaggc cacccugggc ggcuucggca     1140

gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug uacuaccuga     1200

ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccccugcccu     1260

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cugguggagu     1320

ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag gagggcgccg     1380

ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag ggcagacugu     1440

ucagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag ggcgugagcu     1500

acagccugug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc cugcacggca     1560

ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug cccgcccaga     1620

uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc aaccccguga     1680

ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc uucggcgaca     1740

gcuacaucgu gaucggcgug ggcgacaaga agaucaccca ccacuggcac agaagcggca     1800

gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug gccgugcugg     1860

gcgacaccgc cugggacuuc ggcagcgugg gcggcguguu caacagccug ggcaagggca     1920

uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc ugguucagcc     1980

agauccugau cggcacccug cugguguggc ugggccugaa caccaagaac ggcagcauca     2040

gccugaccug ccuggcccug ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6551
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 2 opt

<400>  6551
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cgugggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6552
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 2 opt

<400>  6552
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cgugggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccagcac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac aucggccacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgugug caccgccgcc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6553
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 2 opt

<400>  6553
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau cgugggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac gagaacagag     1080

ccaaggugga ggugaccccc aacagcccca gagccgaggc cacccugggc ggcuucggca     1140

gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug uacuaccuga     1200

ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc ccccugcccu     1260

ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc cugguggagu     1320

ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag gagggcgccg     1380

ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag ggcagacugu     1440

ucagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag ggcgugagcu     1500

acagccugug caccgccgcc uucaccuuca ccaaggugcc cgccgagacc cugcacggca     1560

ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug cccgcccaga     1620

uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc aaccccguga     1680

ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc uucggcgaca     1740

gcuacaucgu gaucggcgug ggcgacaaga agaucaccca ccacuggcac agaagcggca     1800

gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug gccgugcugg     1860

gcgacaccgc cugggacuuc ggcagcgugg gcggcguguu caacagccug ggcaagggca     1920

uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc ugguucagcc     1980

agauccugau cggcacccug cugguguggc ugggccugaa caccaagaac ggcagcauca     2040

gccugaccug ccuggcccug ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6554
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 2 opt

<400>  6554
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgagauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

agaccagaag aagcagaaga agcgugagcc ugagauacca cuacaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaagaa gcaccugauc augguggaga      480

acuggaucuu cagaaacccc ggcuucgcca ucgugagcgu ggccaucacc uggcugaugg      540

gcagccugac cagccagaag gugaucuacc uggugaugau cgugcugauc gugcccgccu      600

acagcaucag cugcaucggc gugagcaaca gagaccuggu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgagauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccauga ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagccug agcgacaugg ccagcgccag cagaugcccc acccagggcg      840

agcccagccu ggacaagcag agcgacaccc agagcgugug caagagaacc cugggcgaca      900

gaggcugggg caacggcugc ggcaucuucg gcaagggcag ccuggugacc ugcagcaagu      960

ucaccugcug caagaagaug cccggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugcc cgugcacggc agccagcaca gcggcaugau cgugaacgac aucggccacg     1080

agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aaggugcccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagag cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcguguuca     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6555
<211>  2446
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 2 opt

<400>  6555
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcg       60

ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc agaagaggca      120

gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc uuccccacca      180

cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug ugcgacgcca      240

ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac guggacugcu      300

ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag aagggcgagg      360

ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag cugcagacca      420

gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga guggagaacu      480

ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg cugcugggca      540

gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc cccgccuaca      600

gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc ggcggcaccu      660

ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag gacaagccca      720

ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug agaagcuacu      780

gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc cagggcgagg      840

ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug guggacagag      900

gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc gccaaguucg      960

ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag uacagaauca     1020

ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc ggccacgaga     1080

ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc gaggccaccc     1140

ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug gacuucagcg     1200

accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag ugguuccacg     1260

acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg aacaacaagg     1320

aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug gugcugggca     1380

gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag auggacggcg     1440

ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac aagcugagac     1500

ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag auccccgccg     1560

agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac ggccccugca     1620

aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc agacugauca     1680

ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug gagcuggacc     1740

cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc acccaccacu     1800

ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga ggcgccaaga     1860

gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc gcccugaaca     1920

gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug uucggcggca     1980

ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc cugaacacca     2040

agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc uuccugagca     2100

ccgccgugag cgccugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2160

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2220

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2280

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2400

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2446


<210>  6556
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 2 opt

<400>  6556
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

gaagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6557
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 2 opt

<400>  6557
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggauguu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6558
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 2 opt

<400>  6558
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caugaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6559
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 2 opt

<400>  6559
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgaggug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgccauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugcu ggugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggcccacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgccaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagaccgu ggacggcacc gugaccgugg agggccagua cggcggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagauca ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcggcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6560
<211>  2440
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 2 opt

<400>  6560
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacgccag caucggcauc gugagccugc ugcugaccac cgugauggcc gccgagauca      120

ccagaagagg cagcgccuac uacauguacc uggacagaag cgacgccggc aaggccauca      180

gcuucgugac cacccugggc gugaacaagu gccacgugca gaucauggac cugggccaca      240

ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc ugccaccaca      360

agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac agcaccagaa      420

agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag caccugauca      480

agguggagaa cuggaucuuc agaaaccccg gcuucgcccu gguggccgug gccaucgccu      540

ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc cugcugaucg      600

cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug gagggcauga      660

gcggcggcac cuggguggac guggugcugg agcacggcgg cugcgugacc gugauggccc      720

aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac auggccgagg      780

ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc agaugcccca      840

cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc aagagaaccc      900

ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc cuggugaccu      960

gcgccaaguu caccugcagc aagaagauga ccggcaagag cauccagccc gagaaccugg     1020

aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc gugaacgacg     1080

agaacagagc caagguggag gugaccccca acagccccag agccgaggcc acccugggcg     1140

gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu     1200

acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc cacgacaucc     1260

cccugcccug gcacagcggc gccgacaccg agacccccca cuggaacaac aaggaggccc     1320

ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug ggcagccagg     1380

agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac ggcgccaagg     1440

gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug agacugaagg     1500

gcgugagcua cagccugugc accgccgccu ucaccuucac caaggugccc gccgagaccc     1560

ugcacggcac cgugaccgug gaggugcagu acgccggcag agacggcccc ugcaaggugc     1620

ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug aucaccgcca     1680

accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug gacccccccu     1740

ucggcgacag cuacaucgug aucggcgugg gcgacaagaa gaucacccac cacuggcaca     1800

gaagcggcag caucaucggc aaggccuucg aggccaccgu gagaggcgcc aagagaaugg     1860

ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcguguuc aacagccugg     1920

gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc ggcaugagcu     1980

gguucagcca gauccugauc ggcacccugc ugguguggcu gggccugaac accaagaacg     2040

gcagcaucag ccugaccugc cuggcccugg gcggcgugau gaucuuccug agcaccgccg     2100

ugagcgccug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2160

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2220

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2280

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2400

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2440


<210>  6561
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 2 opt

<400>  6561
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag caucggcauc aucggccugc ugcugaccac cgccauggcc gccgagauca      120

ccagaagagg cagcgccuac uacauguacc uggacagaag cgacgccggc aaggccauca      180

gcuucgccac caccaugggc gugaacaagu gccacgugca gaucauggac cugggccaca      240

ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug gagcccgacg      300

acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc ugccaccaca      360

agaagggcga ggccagaccc agcagaagag ccgugacccu gcccagccac agcaccagaa      420

agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag caccugauca      480

agguggagaa cuggauccug agaaaccccg gcuucaccgu ggccgcccug gccauggccu      540

ggcugcuggg caacagcacc agccagagag ugaucuaccu ggugaugauc cugcugaucg      600

cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug gagggcauga      660

gcggcggcac cuggguggac guggugcugg agcacggcgg cugcgugacc gugauggccc      720

aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac auggccgagg      780

ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc agaugcccca      840

cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc aagagaaccc      900

ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc cuggugaccu      960

gcgccaaguu caccugcagc aagaagauga ccggcaagag cauccagccc gagaaccugg     1020

aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc gugaacgaca     1080

ucggccacga gaccgacgag aacagagcca agguggaggu gacccccaac agccccagag     1140

ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc agaaccggcc     1200

uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga cagaccgugg     1380

uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg     1440

agauggacgg cgccaagggc agacuguuca gcggccaccu gaagugcaga aucaagaugg     1500

acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc accuucagca     1560

aggugcccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac gccggcagag     1620

acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug acccccgugg     1680

gcagacugau caccgccaac cccaucauca ccgagagcac cgagaacagc agaaugaugc     1740

uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc gacaagaaga     1800

ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag gccaccguga     1860

gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc agcgugggcg     1920

gcguguucaa cagccugggc aagggcgugc accagguguu cggcgccgcc uucaagagcc     1980

uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug guguggcugg     2040

gccugaacac caagaacggc agcaucagcc uggccugccu ggcccugggc ggcgccauga     2100

ucuuccugag caccgccgug agcgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6562
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 2 opt

<400>  6562
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6563
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 2 opt

<400>  6563
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6564
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 2 opt

<400>  6564
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggacaga gccaaggugg      600

aggugacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc      660

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca      720

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg      780

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg      840

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg      900

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug uucagcggcc      960

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1080

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1140

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1200

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1260

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1320

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6565
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 2 opt

<400>  6565
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6566
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 2 opt

<400>  6566
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg cuacgagacc gacgagaaca      600

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcaagc      960

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag auccccgugc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6567
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 2 opt

<400>  6567
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaccguga acgacaucgg cuacgagacc gacgagaaca      600

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcaagc      960

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag auccccgugc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6568
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 2 opt

<400>  6568
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacgagaa cagagccaag guggagguga      600

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu      660

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc      720

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg      780

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg      840

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg      900

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc ggccaccuga      960

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1020

ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug accguggagg     1080

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1140

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1200

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1260

gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1320

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1380

acuucggcag cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  6569
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 2 opt

<400>  6569
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucggcu acgagaccga cgagaacaga gccaaggugg      600

aggugacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc      660

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca      720

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg      780

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg      840

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg      900

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcaagcug uucagcggcc      960

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1020

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1080

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1140

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1200

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1260

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1320

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1380

ccugggacuu cggcagcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6570
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 2 opt

<400>  6570
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc cugagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6571
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 2 opt

<400>  6571
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagca       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6572
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 2 opt

<400>  6572
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg uacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6573
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 2 opt

<400>  6573
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcaagc      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6574
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 2 opt

<400>  6574
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacacccag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6575
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 2 opt

<400>  6575
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgagaucag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6576
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 2 opt

<400>  6576
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccagaaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6577
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 2 opt

<400>  6577
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggugagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6578
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 2 opt

<400>  6578
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaucgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6579
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 2 opt

<400>  6579
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc aucggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6580
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 2 opt

<400>  6580
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccu ggccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6581
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 2 opt

<400>  6581
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcacc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6582
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 2 opt

<400>  6582
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcuggccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6583
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 2 opt

<400>  6583
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga ggcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6584
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 2 opt

<400>  6584
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccgcccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6585
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 2 opt

<400>  6585
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugag caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

ccaucagcga caucgccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgccguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6586
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 2 opt

<400>  6586
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucagcaagau ccccgccgag acccugcacg     1080

gcaccgugac cgaggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6587
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 2 opt

<400>  6587
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggagcug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6588
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 2 opt

<400>  6588
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcaucag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcauccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6589
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 2 opt

<400>  6589
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgug aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6590
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 2 opt

<400>  6590
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcgccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6591
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 2 opt

<400>  6591
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacac cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gggcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6592
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 2 opt

<400>  6592
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccgcca uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augcugguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggccc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgcc accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag accguggacg     1080

gcaccgugac cguggagggc caguacggcg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6593
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 2 opt

<400>  6593
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccgcc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6594
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 2 opt

<400>  6594
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugaa caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6595
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 2 opt

<400>  6595
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcgccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6596
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 2 opt

<400>  6596
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6597
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 2 opt

<400>  6597
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg uacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6598
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 2 opt

<400>  6598
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6599
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 2 opt

<400>  6599
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca      600

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6600
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 2 opt

<400>  6600
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca      600

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gugugcaccg ccgccaaggu gcccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6601
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 2 opt

<400>  6601
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacgagaa cagagccaag guggagguga      600

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu      660

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc      720

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac agcggcgccg      780

acaccgagac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg      840

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg      900

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga      960

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1020

ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug accguggagg     1080

ugcaguacgc cggcagagac ggccccugca aggugcccgc ccagauggcc guggacaugc     1140

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1200

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1260

gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcauc aucggcaagg     1320

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1380

acuucggcag cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  6602
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 2 opt

<400>  6602
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccuggug augaucgugc ugaucgugcc cgccuacagc aucagcugca      120

ucggcgugag caacagagac cugguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccgaga uggcccagga caagcccacc guggacaucg      240

agcuggugac caugaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gccugagcga cauggccagc gccagcagau gccccaccca gggcgagccc agccuggaca      360

agcagagcga cacccagagc gugugcaaga gaacccuggg cgacagaggc uggggcaacg      420

gcugcggcau cuucggcaag ggcagccugg ugaccugcag caaguucacc ugcugcaaga      480

agaugcccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugcccgugc      540

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca      600

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag acccugcacg     1080

gcaccgugac cguggaggug cagagcgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6603
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 2 opt

<400>  6603
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      600

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1200

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6604
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 2 opt

<400>  6604
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agagagugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      120

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      180

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      240

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      300

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      360

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      420

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      480

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      540

acggcagcca gcacagcggc augaucguga acgacaucgg ccacgagacc gacgagaaca      600

gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      660

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      720

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc      780

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg      840

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg      900

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac      960

uguucagcgg ccaccugaag ugcagaauca agauggacaa gcugagacug aagggcguga     1020

gcuacagccu gugcaccgcc gccuucaccu ucagcaaggu gcccgccgag acccugcacg     1080

gcaccgugac cguggaggug caguacgccg gcagagacgg ccccugcaag gugcccgccc     1140

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaacccca     1200

ucaucaccga gagcaccgag aacagcagaa ugaugcugga gcuggacccc cccuucggcg     1260

acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg cacagaagcg     1320

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1380

ugggcgacac cgccugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6605
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  6605
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacauug      720

ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca guugacaucg      780

agcuugugac caccacagug uccaauaugg ccgaagucag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ugaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcgcacgcu aagagacaga cgguggucgu gcucggcucu caggaggggg     1440

ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

ugauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc gcuguuucug     2160

ccgacguggg gugcuccguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6606
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 opt

<400>  6606
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacacaug ugacgccacc augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacaa      360

ccuccacgug ggucguguau ggaaccuguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacacuuccc agccauucca cccgcaagcu gcaaacaaga ucucagaccu      480

ggcucgagag ccgggaauac acgaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacaucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaaccugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcucagga uaagccuacc guggacauug      780

agcuugugac cacaacgguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccaccca aggagaagcc uaccuggaca      900

aacagucuga uacacaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ucacaugcgc uaaguuugcg ugcuccaaga     1020

aaaugaccgg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugugc     1080

acggcuccca acacagcggg augaucguua acgauacggg acaugagaca gacgagaaua     1140

gagccaaagu ggaaauuacc ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga caggccugga cuuuuccgau cuuuauuacc     1260

ugaccaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau acaggaacgc cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga ccgucguggu ucucggcucu caggaggggg     1440

ccgugcauac agcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguaccgcg gccuucacau uuaccaaaau uccggcugaa acgcugcaug     1620

gcaccgugac aguugaggug caguacgccg ggaccgacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acacugaccc cugugggccg ccucaucacg gcgaaccccg     1740

uuaucacaga auccaccgag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac agccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaacg cuccugaugu ggcugggccu caaugccaag aacgggagca     2100

ucucccugau gugucuugcu cugggaggcg uucucauuuu ccugagcacc gccgugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6607
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opt

<400>  6607
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc cugggaguga      240

auaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu gacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugauuaaagu ugaaaauugg aucuuuagaa      540

accccggcuu cgcccuggug gcugucgcca uugcuuggcu ccuggggagc ucuacguccc      600

agaaggugau cuaccuuguu augauucugc ucaucgcccc ugcguauagc auucggugca      660

ucggaguguc uaaucgcgau uuugucgagg gcauguccgg gggaacaugg guggacgugg      720

uccuggaaca cggcgggugu guuaccguga uggcccagga uaaacccaca guggacaucg      780

agcuugucac caccacagug agcaacaugg cugagguuag aucuuacugc uacgaagccu      840

ccauuagcga uauggcuucc gacucucggu guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gugugcaaaa gaacacucgu cgaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg ugaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauc cagcccgaaa accucgagua ucggauuaug cuguccguuc     1080

acggcagcca acauucuggg augaucggau acgaaacgga ugaggacaga gcuaaggugg     1140

aggucacacc uaauagcccg cgcgcggaag ccacccuugg cggcuucggg ucccugggac     1200

ucgauuguga gccccggacc ggccuggacu uuucugaucu uuauuaccug acaaugaaca     1260

auaaacacug gcucgugcau aaggaauggu uccacgacau uccucugccc uggcacgcug     1320

gggccgauac cggaacaccu cauuggaaca auaaagaggc ccuggucgaa uuuaaggacg     1380

cucacgccaa gagacagacg gugguugugc ucggcagcca ggagggggcg guccauaccg     1440

cucuggccgg cgcccuugag gcugaaaugg auggagccaa aggccgccug uucuccgggc     1500

accucaagug ccggcugaaa auggacaaac uuagacugaa gggagugagc uauucucucu     1560

guacagcugc cuuuaccuuc acaaagguuc ccgcggagac ccugcauggc acggugaccg     1620

ucgaagugca guacgccggg acagacggac cgugcaagau cccugugcaa auggcuguug     1680

auaugcagac ccugacaccc gucggccgcc ucaucaccgc caacccugug auuacggagu     1740

ccacagaaaa uagcaaaaug augcuggagc uugacccucc cuuuggggau ucuuacaucg     1800

ugauuggcgu cggagacaag aaaaucaccc accacuggca ucgguccggc agcacaauug     1860

ggaaggcuuu cgaggccacc gugagaggag cgaaacgcau ggccguucug ggcgauaccg     1920

cuugggacuu cggguccgug ggaggcgucu uuaacucucu cgggaagggc auccaccaga     1980

ucuucggagc cgcuuuuaag agccuguucg gcgggauguc cugguuuucu caaauucuga     2040

ucggaacacu ucucgugugg cugggccuga acacgaaaaa ugggagcauu ucccucaccu     2100

gucuggcccu uggaggcguu augaucuucc ugagcacagc cgugucugcu gaugucgggu     2160

gcuccgugga cuuuagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6608
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 2 opt

<400>  6608
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6609
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  6609
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6610
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 2 opt

<400>  6610
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac acaugugaug      240

ccaccaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa cacaaccucc acgugggucg uguaugggac cugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuacac uucccagcca uuccacccgg aaacugcaaa      420

cacgcucuca gaccuggcuc gagagcagag aguacacgaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac aucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

ccugggucga uguggugcug gaacauggag gcugugucac aguuauggcg caggacaagc      720

cuaccgugga uauugagcuu gugaccacaa cggucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acccaagggg      840

aggcuuaccu ggacaagcag ucugacacac aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggucaca ugcgccaaau      960

uugccugcuc caagaaaaug accgggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac acggggcaug     1080

aaacagauga gaaccgggcu aagguggaga uuacccccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcacagga cuggauuuuu     1200

ccgaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacacagg gacgccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagaccguc gugguucucg     1380

gaucucagga gggcgcggug cauacagcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua ccgcugccuu cacauuuacc aagauuccgg     1560

cggagacgcu gcauggaacc gugacaguug aagugcagua cgccggcacc gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacggcuaa ccccguuauu acagagucca ccgaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauacagccu gggacuucgg cuccguuggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacgcuccu gauguggcug ggacucaacg     2040

cuaagaaugg cagcaucucc cugauguguc uugcgcuugg gggagugcuc auuuuccuga     2100

gcaccgccgu gucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6611
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 2 opt

<400>  6611
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauc aagguugaga      480

acuggaucuu ucggaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauucg cugcaucggg gugucuaaca gagacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcacggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucggucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaaagaaca cucgucgauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

uuaugcuguc cguucacgga agccaacauu cuggcaugau cggguacgag acggacgaag     1080

auagagccaa gguggagguc acaccuaaca gcccgcgggc cgaggcuacc cuuggaggcu     1140

ucggcucccu ggggcucgac ugugaaccca gaaccggacu ggauuuuucu gaccuuuauu     1200

accugacaau gaauaacaaa cacuggcucg ugcauaagga gugguuccac gauauuccuc     1260

ugcccuggca cgcgggcgcc gacaccggga caccucauug gaauaacaaa gaagcucugg     1320

ucgaguuuaa ggaugcccac gccaaacgcc agacgguggu ugugcucgga agccaggaag     1380

gcgcugucca uaccgcccug gcgggggcuc uugaggccga gauggacggc gccaagggac     1440

ggcuguucuc cggccaccuc aagugcagac ugaaaaugga uaaacuucgc cugaaggggg     1500

ugagcuauuc ucucuguaca gcugccuuua ccuucacaaa gguucccgcu gaaacccugc     1560

auggaacggu gaccgucgag gugcaguacg ccggcacaga cgggccgugc aaaaucccug     1620

ugcaaauggc gguugacaug cagacccuga cacccgucgg acggcucauu accgccaauc     1680

cugugaucac ggaauccaca gagaacagca agaugaugcu ggaacuugau ccucccuuug     1740

gcgacucuua caucgugauu ggggucggcg auaagaaaau cacccaccac uggcauagau     1800

ccggaagcac aauuggcaag gcuuucgagg ccaccgugcg cggggcuaaa cggauggccg     1860

uucugggaga caccgcgugg gauuucggcu ccgucggggg aguguuuaau ucucucggca     1920

aggggaucca ccagauuuuc ggcgccgcuu uuaaaagccu guucggaggc auguccuggu     1980

uuucucaaau ccugaucggg acacuucucg uguggcuggg acugaacacg aagaacggca     2040

gcauuucccu caccugucug gcccuugggg gaguuaugau cuuccugagc acagcugugu     2100

cugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6612
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 2 opt

<400>  6612
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6613
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 opt

<400>  6613
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggauauug      180

uccuugaaca cgggggaugu gugacaguua uggcccaaga caaaccuacg guggauaucg      240

agcuggucac cacaaccgug uccaauaugg cggaaguucg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gugugcaaac ggacacuggu cgaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauu caacccgaga aucucgagua cagaaucaug cugagcgugc      540

augggucuca gcacuccgga augauuguca acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ugaaaucacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac auuccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggugg      840

aguucaagga ugcucacgcc aagcggcaga cagucguggu ucucgggagc caggaaggag      900

cggugcauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguca     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ccccgcggag acccuucacg     1080

ggacagugac cguugaagug caauaugccg gaacagacgg cccuuguaaa guccccgcuc     1140

agauggccgu ggauaugcag acccugacgc cugucgggcg ccucauuaca gccaaucccg     1200

ugaucaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cguuauuggc gugggggaaa agaaaaucac acaucacugg cauagaagcg     1320

gcucuaccau uggaaaggcu uucgaggcca caguccgggg cgcgaaacgc auggcugugc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6614
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 opt

<400>  6614
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6615
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opt

<400>  6615
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucggcu augaaacaga ugaggaccgg gccaagguug      600

aagugacccc uaacagcccc cgcgcugagg ccacacucgg gggauuuggc ucucugggcc      660

uggauugcga gccuagaacc gggcuugacu ucuccgaucu guacuaccuc acgaugaaua      720

acaaacauug gcuggugcac aaggaauggu uucaugacau uccccuuccu uggcacgcgg      780

gagccgacac aggcaccccg cacuggaaua acaaagaggc ucuggucgag uucaaggaug      840

cccaugcuaa gcggcagaca gugguugugc ucgggagcca ggaaggagcc guccacaccg      900

cgcuggccgg cgcucuggaa gccgagaugg acggggcuaa aggacgccuc uuuuccggcc      960

aucugaagug uagacuuaaa auggauaagc ugcggcucaa aggcgugucu uauagccugu     1020

gcaccgccgc cuucacauuu acgaagguuc ccgcugagac ccuucacggg acagugaccg     1080

ucgaagugca auacgcggga acagacggcc cuuguaaaau ccccguccag auggccgugg     1140

auaugcagac ccugacgccu guugggcgcc ucauuacagc uaaucccgug aucaccgagu     1200

ccaccgaaaa cagcaagaug augcuggagc uggacccgcc uuucggagau ucuuauauug     1260

ucaucggcgu gggggacaag aaaaucacac accauuggca cagauccggc agcaccauug     1320

gaaaggccuu ugaagccaca guucggggcg cuaaacgcau ggccgugcuc ggggauacgg     1380

cgugggacuu cggaucuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6616
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 2 opt

<400>  6616
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6617
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       2 opt

<400>  6617
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agccucuauu uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcugagcg gaaagggcuc ccuggugacc ugcgccaaau      960

ucgccugcag caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc cguccacgga agccaacacu cuggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacaccgaa uagccccaga gcggaagcca     1140

cccuuggagg cuuuggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuucu     1200

cugaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguuuc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaguuc aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaagccagga aggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugucuuau ucccucugua cagcugccuu uaccuucaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagagca cagaaaacuc uaagaugaug cuggagcuug     1740

aucccccuuu uggcgacucc uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cagcggaucc acaauuggca aggccuucga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuuugg cucugucggg ggagcucuca     1920

auagccuggg caaggggauc caccagauuu ucggcgccgc cuucaaaucc cuguuuggag     1980

gcaugucuug guucagccaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cuccauuagc cugauguguc uugcucuugg gggagugcuc aucuuucugu     2100

cuaccgcggu uuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6618
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 2
       opt

<400>  6618
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac acaugugaug      240

ccaccaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa cacaaccucc acgugggucg uguaugggac cugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuacac uucccagcca uuccacccgg aaacugcaaa      420

cacgcucuca gaccuggcuc gagagcagag aguacacgaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac aucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

ccugggucga uguggugcug gaacauggag gcugugucac aguuauggcg caggacaagc      720

cuaccgugga uauugagcuu gugaccacaa cggucuccaa cauggccgag gugcgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acccaagggg      840

aggcuuaccu ggacaagcag ucugacacac aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcugagcg gaaagggcuc ccuggucaca ugcgccaaau      960

ucgccugcag caagaaaaug accgggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc cgugcacgga agccaacacu cuggcaugau cguuaaugac acggggcaug     1080

aaacagauga gaaccgggcu aagguggaga uuaccccgaa uagccccaga gcggaagcca     1140

cccuuggagg cuuuggcucc cuggggcucg acugugagcc ucgcacagga cuggauuucu     1200

cugaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguuuc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacacagg gacgccccau uggaacaaua     1320

aagaggcccu gguggaguuc aaggaugcuc acgccaaacg gcagaccguc gugguucucg     1380

gaagccagga aggcgcggug cauacagcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucucuuau ucccucugua ccgcugccuu uacauucacc aagauuccgg     1560

cggagacgcu gcauggaacc gugacaguug aagugcagua cgccggcacc gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacggcuaa ccccguuauu acagagagca ccgaaaacuc uaagaugaug cuggagcuug     1740

aucccccuuu uggcgacucc uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cagcggaucc accaucggca aggccuucga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauacagccu gggacuuugg cucuguuggg ggagcucuca     1920

auagccuggg caaggggauu caccagaucu ucggcgccgc cuucaaaucc cuguuuggag     1980

gcaugucuug guucagccaa auucuuaucg ggacgcuccu gauguggcug ggacucaacg     2040

cuaagaaugg cuccaucagc cugauguguc uugcgcuugg gggagugcuc auuuuucugu     2100

cuaccgccgu guccgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6619
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 2 
       opt

<400>  6619
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauc aagguugaga      480

acuggaucuu ucggaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauucg cugcaucggg gugucuaaca gagacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcacggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucggucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaaagaaca cucgucgauc      900

ggggaugggg caacggcugu gggcuguccg gaaagggcag ccuggugacc ugcgcuaaau      960

ucacaugcuc uaagaagaug accgggaaau ccauccagcc cgagaaucuc gaauaucgca     1020

uuaugcugag cguucacgga ucucaacaua gcggcaugau cggguacgag acggacgaag     1080

auagagccaa gguggagguc acaccuaacu ccccgcgggc cgaggcuacc cuuggaggcu     1140

uuggcucucu ggggcucgac ugugaaccca gaaccggacu ggauuucagc gaccuuuauu     1200

accugacaau gaauaacaaa cacuggcucg ugcauaagga gugguuucac gauauuccuc     1260

ugcccuggca cgcgggcgcc gacaccggga caccucauug gaauaacaaa gaagcucugg     1320

ucgaguucaa ggaugcccac gccaaacgcc agacgguggu ugugcucgga ucccaggaag     1380

gcgcugucca uaccgcccug gcgggggcuc uugaggccga gauggacggc gccaagggac     1440

ggcuguuuag cggccaccuc aagugcagac ugaaaaugga uaaacuucgc cugaaggggg     1500

ugucuuauuc ccucuguaca gcugccuuca ccuuuacaaa gguucccgcu gaaacccugc     1560

auggaacggu gaccgucgag gugcaguacg ccggcacaga cgggccgugc aaaaucccug     1620

ugcaaauggc gguugacaug cagacccuga cacccgucgg acggcucauu accgccaauc     1680

cugugaucac ggaaagcaca gagaacucua agaugaugcu ggaacuugau ccucccuucg     1740

gcgacuccua caucgugauu ggggucggcg auaagaaaau cacccaccac uggcauagaa     1800

gcggauccac aauuggcaag gcuuuugagg ccaccgugcg cggggcuaaa cggauggccg     1860

uucugggaga caccgcgugg gauuucggcu cugucggggg aguguucaau agccucggca     1920

aggggaucca ccagauuuuu ggcgccgcuu ucaaaucccu guuuggaggc augucuuggu     1980

ucagccaaau ccugaucggg acacuucucg uguggcuggg acugaacacg aagaacggcu     2040

ccauuagccu caccugucug gcccuugggg gaguuaugau cuuucugucu acagcugugu     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6620
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 2 
       opt

<400>  6620
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcugagcg gaaagggcuc ccuggugacc ugcgccaaau      960

ucgccugcag caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc cgugcacgga agccaacacu cuggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacaccgaa uagccccaga gcggaagcca     1140

cccuuggagg cuuuggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuucu     1200

cugaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguuuc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaguuc aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaagccagga aggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucucuuau ucccucugua cagcugccuu uaccuucaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagagca cagaaaacuc uaagaugaug cuggagcuug     1740

aucccccuuu uggcgacucc uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cagcggaucc acaaucggca aggccuucga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuuugg cucugucggg ggagcucuca     1920

auagccuggg caaggggauu caccagaucu ucggcgccgc cuucaaaucc cuguuuggag     1980

gcaugucuug guucagccaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cuccaucagc cugauguguc uugcucuugg gggaguucuc auuuuucugu     2100

cuaccgcggu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6621
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       2 opt

<400>  6621
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugcaggac accgggcaug     1080

aaacggauga gaaucgggcu aagguugaga ucacaccgaa cucucccaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaauaaca     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucaaua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucagaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ucccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

acucucuggg caaggggauc caccaaauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucag auccuuaucg ggacacuccu gauguggcug ggacucaaua     2040

cgaagaacgg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6622
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 2
       opt

<400>  6622
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac acaugugaug      240

ccaccaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa cacaaccucc acgugggucg uguaugggac cugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuacac uucccagcca uuccacccgg aaacugcaaa      420

cacgcucuca gaccuggcuc gagagcagag aguacacgaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac aucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

ccugggucga uguggugcug gaacauggag gcugugucac aguuauggcg caggacaagc      720

cuaccgugga uauugagcuu gugaccacaa cggucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acccaagggg      840

aggcuuaccu ggacaagcag ucugacacac aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggucaca ugcgccaaau      960

uugccugcuc caagaaaaug accgggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguucaggac acggggcaug     1080

aaacagauga gaaucgggcu aagguggaga uuaccccgaa cucucccaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcacagga cuggauuuuu     1200

ccgaccuuua uuaccugacc augaauaaca aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacacagg gacgccccau uggaauaaca     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagaccguc gugguucucg     1380

gaucucagga gggcgcggug cauacagcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua ccgcugccuu cacauuuacc aagauuccgg     1560

cggagacgcu gcauggaacc gugacaguug aagugcaaua cgccggcacc gacgggccuu     1620

gcaaaguccc cgcucagaug gccguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacggcuaa ucccguuauu acagagucca ccgaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauacagccu gggacuucgg cuccguuggg ggagcucuca     1920

acucucuggg caaggggauu caccaaaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucag auucuuaucg ggacgcuccu gauguggcug ggacucaaug     2040

cuaagaacgg cagcaucucc cugauguguc uugcgcuugg gggagugcuc auuuuccuga     2100

gcaccgccgu gucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6623
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 2 
       opt

<400>  6623
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguucaggac accgggcaug     1080

aaacggauga gaaucgggcu aagguggaga uuacaccgaa cucucccaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaauaaca aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaauaaca     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcaaua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucagaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ucccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

acucucuggg caaggggauu caccaaaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucag auucuuaucg ggacacuccu gauguggcug ggacucaaua     2040

cgaagaacgg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6624
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6624
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaaacuuc     1500

ggcugaaggg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acacugggca aggccuuuuc caccacccuc aaaggggcgc     1860

agcggcuggc cgcucuggga gauacagccu gggacuucgg cucuauuggg ggaguguuca     1920

auagcaucgg cagagcugug caccaagucu uugggggcgc cuuccgcacg cuuuuuggag     1980

gcauguccug gauuacccag gggcucaugg gagcccugcu gcucuggaug ggcgugaacg     2040

cucgggauag aucuaucgcg cuggccuucc uugcuacagg gggaguucug guguuucucg     2100

ccaccaaugu ccaugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6625
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6625
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac acaugugaug      240

ccaccaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa cacaaccucc acgugggucg uguaugggac cugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuacac uucccagcca uuccacccgg aaacugcaaa      420

cacgcucuca gaccuggcuc gagagcagag aguacacgaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac aucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

ccugggucga uguggugcug gaacauggag gcugugucac aguuauggcg caggacaagc      720

cuaccgugga uauugagcuu gugaccacaa cggucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acccaagggg      840

aggcuuaccu ggacaagcag ucugacacac aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggucaca ugcgccaaau      960

uugccugcuc caagaaaaug accgggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac acggggcaug     1080

aaacagauga gaaccgggcu aagguggaga uuacccccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcacagga cuggauuuuu     1200

ccgaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacacagg gacgccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagaccguc gugguucucg     1380

gaucucagga gggcgcggug cauacagcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaaacuuc     1500

ggcugaaggg ggucagcuau ucucucugua ccgcugccuu cacauuuacc aagauuccgg     1560

cggagacgcu gcauggaacc gugacaguug aagugcagua cgccggcacc gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacggcuaa ccccguuauu acagagucca ccgaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc acccugggca aggccuuuuc caccacacuc aaaggggcgc     1860

agcggcuggc cgcucuggga gauacggccu gggacuucgg cucuaucggg ggaguguuca     1920

auagcauugg cagagcuguc caccaagugu uugggggcgc cuuccgcacc cuuuuuggag     1980

gcauguccug gaucacacag gggcucaugg gagcccugcu gcucuggaug ggcguuaacg     2040

cucgggauag aucuauugcg cuggccuucc uugcuaccgg gggagugcug gucuuucucg     2100

ccacaaaugu gcaugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6626
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 2 opt

<400>  6626
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauc aagguugaga      480

acuggaucuu ucggaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauucg cugcaucggg gugucuaaca gagacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcacggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucggucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaaagaaca cucgucgauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

uuaugcuguc cguucacgga agccaacauu cuggcaugau cggguacgag acggacgaag     1080

auagagccaa gguggagguc acaccuaaca gcccgcgggc cgaggcuacc cuuggaggcu     1140

ucggcucccu ggggcucgac ugugaaccca gaaccggacu ggauuuuucu gaccuuuauu     1200

accugacaau gaauaacaaa cacuggcucg ugcauaagga gugguuccac gauauuccuc     1260

ugcccuggca cgcgggcgcc gacaccggga caccucauug gaauaacaaa gaagcucugg     1320

ucgaguuuaa ggaugcccac gccaaacgcc agacgguggu ugugcucgga agccaggaag     1380

gcgcugucca uaccgcccug gcgggggcuc uugaggccga gauggacggc gccaagggac     1440

ggcuguucuc cggccaccuc aagugcagac ugaaaaugga uaaacuucgc cugaaggggg     1500

ugagcuauuc ucucuguaca gcugccuuua ccuucacaaa gguucccgcu gaaacccugc     1560

auggaacggu gaccgucgag gugcaguacg ccggcacaga cgggccgugc aaaaucccug     1620

ugcaaauggc gguugacaug cagacccuga cacccgucgg acggcucauu accgccaauc     1680

cugugaucac ggaauccaca gagaacagca agaugaugcu ggaacuugau ccucccuuug     1740

gcgacucuua caucgugauu ggggucggcg auaagaaaau cacccaccac uggcauagau     1800

ccggaagcac acugggcaag gcuuucucca ccacccucaa aggggcccag cgccuggcug     1860

cccugggaga cacagcgugg gauuucggcu cuauuggggg aguguuuaau agcaucggcc     1920

gggccguuca ccaaguguuc gggggcgcuu uuagaacgcu uuucggaggc auguccugga     1980

uuacccaggg gcucauggga gcccugcugc ucuggauggg cgucaacgcu cgcgaccggu     2040

cuaucgcccu ggccuuucuu gcuacagggg gagugcuggu uuuccucgcg accaacgugc     2100

augccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6627
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6627
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaaacuuc     1500

ggcugaaggg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acacugggca aggccuuuuc caccacccuc aaaggggcgc     1860

agcggcuggc cgcucuggga gauacagccu gggacuucgg cucuaucggg ggaguguuca     1920

auagcauugg cagagcuguc caccaagugu uugggggcgc cuuccgcacg cuuuuuggag     1980

gcauguccug gaucacccag gggcucaugg gagcccugcu gcucuggaug ggcguuaacg     2040

cucgggauag aucuauugcg cuggccuucc uugcuacagg gggagugcug gucuuucucg     2100

ccaccaaugu gcaugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6628
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  6628
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacauug      720

ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca guugacaucg      780

agcuugugac caccacagug uccaauaugg ccgaagucag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ugaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcgcacgcu aagagacaga cgguggucgu gcucggcucu caggaggggg     1440

ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

ugauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc gcuguuucug     2160

ccgacguggg gugcuccguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6629
<211>  2521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6629
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uuaacgccag aaaggagaaa aagcggcgcg gcgcugacac cagcgugggg aucgucggac      120

ugcuccugac aacggccaug gcggcugaag uuaccagacg gggcuccgcc uacuauaugu      180

accuugaucg caaugacgcc ggggaggcua uuucuuuccc cacaacccug ggaaugaaca      240

aaugcuauau ccagauuaug gaucucggcc acauguguga cgccacaaug agcuacgaau      300

gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc aauaccacau      360

ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggcuaga cggucucgca      420

gagccguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu cagacauggc      480

ucgagagcag agaauacacc aagcaccuga uccgggugga gaacuggauc uuucgcaauc      540

cuggcuucgc gcuggccgcu gccgcuauug ccuggcuccu gggguccagc acgucucaga      600

aagucaucua ucuugugaug auucugcuca ucgcgcccgc cuacuccauu agaugcaucg      660

gaguuagcaa ccgggauuuu guggaaggca ugucuggggg aacauggguc gacauugugc      720

uggagcaugg cgggugugug accgucaugg cucaggauaa gccuacaguu gacaucgagc      780

uugugaccac cacagugucc aauauggccg aaguccgcag cuacugcuau gaggcuucua      840

ucuccgauau ggccagcgac uccagauguc ccacgcaagg agaagccuac cuggacaaac      900

agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg ggcaacgggu      960

guggacuguu cggcaaaggg agccugguga ccugcgcuaa guuugcgugc uccaagaaaa     1020

ugacaggaaa gagcauucag ccugagaauc ucgaauaccg caucaugcug ucuguccacg     1080

gcucccaaca cagcgggaug auugugaacg auaccggaca ugagacggac gagaaucggg     1140

ccaaaguuga aaucacaccg aacucuccca gagcugaggc cacccuuggc ggcuucggga     1200

gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu uauuaccuga     1260

caaugaauaa caagcacugg cucgugcaua aagagugguu ccacgacauu ccccugccuu     1320

ggcacgccgg ggcugauacc ggaacacccc auuggaauaa caaggaagcc cuggucgagu     1380

uuaaagacgc gcacgcuaag cggcagacgg uggucgugcu cggcucucag gagggggccg     1440

uucauaccgc ccuggcuggc gcccuugaag cugagaugga uggagccaag ggcagacuga     1500

gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag ggagugagcu     1560

auucucucug uacagcggcc uucaccuuua caaaaauccc ggcugaaacc cugcauggca     1620

cggucaccgu ggagguucag uacgccggga cagacggacc uugcaaggug cccgcucaaa     1680

uggccgucga uaugcagacc cugacaccug ugggcagacu caucaccgcg aaccccguga     1740

uuacggaauc cacagagaau agcaagauga ugcuggagcu ugaucccccu uucggggacu     1800

cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau cgcuccggca     1860

gcacaauugg gaaagccuuu gaagcuaccg ugcggggagc caagagaaug gcugugcugg     1920

gcgacaccgc cugggauuuc ggguccgucg gaggcgcccu caacucucug gggaaaggca     1980

uccaccagau cuucggagcu gcguuuaaga gccuguucgg cgggaugucc ugguuuucuc     2040

aaauucuuau cggaacacuc cugauguggc ugggccucaa uacgaagaac gggagcauuu     2100

cccugaugug ucuugcccug ggaggcgugc ucaucuuccu gagcaccgcu guuucugccg     2160

acguggggug cuccgucgau uuuagcaaau gaggacuagu gcaucacauu uaaaagcauc     2220

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2280

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2340

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2460

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2520

u                                                                     2521


<210>  6630
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6630
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaaaaag cggcgcggcg cugacaccag cguggggauu gucggacugc      120

uccugacaac ggccauggcg gcugaaguua ccagacgggg cuccgccuac uauauguacc      180

uugaucgcaa ugacgccggg gaggcuaucu cuuuccccac aacccuggga augaacaaau      240

gcuauauuca gaucauggau cucggccaca ugugugacgc cacaaugagc uacgaaugcc      300

cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau accacaucca      360

ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcuagacgg ucucgcagag      420

ccguuacccu ucccagccau uccacacgga agcugcaaac ccgcucucag acauggcucg      480

agagcagaga auacaccaag caccugauuc ggguggagaa cuggaucuuu cgcaauccug      540

gcuucgcgcu ggccgcugcc gcuaucgccu ggcuccuggg guccagcacg ucucagaaag      600

ucauuuaucu ugugaugauc cugcucauug cgcccgccua cuccaucaga ugcauuggag      660

uuagcaaccg ggauuuugug gaaggcaugu cugggggaac augggucgac aucgugcugg      720

agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac auugagcuug      780

ugaccaccac aguguccaau auggccgaag uccgcagcua cugcuaugag gcuucuaucu      840

ccgauauggc cagcgacucc agauguccca cgcaaggaga agccuaccug gacaaacagu      900

cugauaccca guaugugugc aagcggacac ucguugacag aggcuggggc aacgggugug      960

gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc aagaaaauga     1020

caggaaagag cauccagccu gagaaucucg aauaccgcau uaugcugucu guccacggcu     1080

cccaacacag cgggaugauc gugaacgaua ccggacauga gacggacgag aaucgggcca     1140

aaguugaaau uacaccgaac ucucccagag cugaggccac ccuuggcggc uucgggagcc     1200

ugggacucga uugugaaccu cgcaccggcc uggacuuuuc cgaucuuuau uaccugacaa     1260

ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacaucccc cugccuuggc     1320

acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug gucgaguuua     1380

aagacgcgca cgcuaagcgg cagacggugg ucgugcucgg cucucaggag ggggccguuc     1440

auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc agacugagcu     1500

ccgggcaccu caaaugccgc cugaagaugg acaaacuucg gcugaaggga gugagcuauu     1560

cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug cauggcacgg     1620

ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc gcucaaaugg     1680

ccgucgauau gcagacccug acaccugugg gcagacucau caccgcgaac cccgugauca     1740

cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc ggggacucuu     1800

acauuguuau cggcgucgga gagaaaaaga uuacccacca cuggcaucgc uccggcagca     1860

caaucgggaa agccuuugaa gcuaccgugc ggggagccaa gagaauggcu gugcugggcg     1920

acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg aaaggcauuc     1980

accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg uuuucucaaa     2040

uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg agcaucuccc     2100

ugaugugucu ugcccuggga ggcgugcuca uuuuccugag caccgcuguu ucugccgacg     2160

uggggugcuc cgucgauuuu agcaaaugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  6631
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6631
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaaaaagcgg cgcggcgcug acaccagcgu ggggaucguc ggacugcucc      120

ugacaacggc cauggcggcu gaaguuacca gacggggcuc cgccuacuau auguaccuug      180

aucgcaauga cgccggggag gcuauuucuu uccccacaac ccugggaaug aacaaaugcu      240

auauccagau uauggaucuc ggccacaugu gugacgccac aaugagcuac gaaugcccua      300

ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaauacc acauccaccu      360

gggucgugua uggaacgugu caucacaaga aaggcgaggc uagacggucu cgcagagccg      420

uuacccuucc cagccauucc acacggaagc ugcaaacccg cucucagaca uggcucgaga      480

gcagagaaua caccaagcac cugauccggg uggagaacug gauuuuucgc aauccuggcu      540

ucgcgcuggc cgcugccgcu aucgccuggc uccugggguc cagcacgucu cagaaaguca      600

ucuaucuugu gaugauucug cucaucgcgc ccgccuacuc cauuagaugc aucggaguua      660

gcaaccggga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu gugcuggagc      720

auggcgggug ugugaccguc auggcucagg auaagccuac aguugacauc gagcuuguga      780

ccaccacagu guccaauaug gccgaagucc gcagcuacug cuaugaggcu ucuauuuccg      840

auauggccag cgacuccaga ugucccacgc aaggagaagc cuaccuggac aaacagucug      900

auacccagua ugugugcaag cggacacucg uugacagagg cuggggcaac ggguguggac      960

uguucggcaa agggagccug gugaccugcg cuaaguuugc gugcuccaag aaaaugacag     1020

gaaagagcau ccagccugag aaucucgaau accgcaucau gcugucuguc cacggcuccc     1080

aacacagcgg gaugauugug aacgauaccg gacaugagac ggacgagaau cgggccaaag     1140

uugaaaucac accgaacucu cccagagcug aggccacccu uggcggcuuc gggagccugg     1200

gacucgauug ugaaccucgc accggccugg acuuuuccga ucuuuauuac cugacaauga     1260

auaacaagca cuggcucgug cauaaagagu gguuccacga cauuccccug ccuuggcacg     1320

ccggggcuga uaccggaaca ccccauugga auaacaagga agcccugguc gaguuuaaag     1380

acgcgcacgc uaagcggcag acgguggucg ugcucggcuc ucaggagggg gccguucaua     1440

ccgcccuggc uggcgcccuu gaagcugaga uggauggagc caagggcaga cugagcuccg     1500

ggcaccucaa augccgccug aagauggaca aacuucggcu gaagggagug agcuauucuc     1560

ucuguacagc ggccuucacc uuuacaaaaa ucccggcuga aacccugcau ggcacgguca     1620

ccguggaggu ucaguacgcc gggacagacg gaccuugcaa ggugcccgcu caaauggccg     1680

ucgauaugca gacccugaca ccugugggca gacucauuac cgcgaacccc gugaucacgg     1740

aauccacaga gaauagcaag augaugcugg agcuugaucc cccuuucggg gacucuuaca     1800

ucguuauugg cgucggagag aaaaagauca cccaccacug gcaucgcucc ggcagcacaa     1860

uugggaaagc cuuugaagcu accgugcggg gagccaagag aauggcugug cugggcgaca     1920

ccgccuggga uuucgggucc gucggaggcg cccucaacuc ucuggggaaa ggcauccacc     1980

agauuuucgg agcugcguuu aagagccugu ucggcgggau guccugguuu ucucaaaucc     2040

uuaucggaac acuccugaug uggcugggcc ucaauacgaa gaacgggagc auuucccuga     2100

ugugucuugc ccugggaggc gugcucaucu uccugagcac cgcuguuucu gccgacgugg     2160

ggugcuccgu cgauuuuagc aaaugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6632
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6632
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa aaagcggcgc ggcgcugaca ccagcguggg gaucgucgga cugcuccuga      120

caacggccau ggcggcugaa guuaccagac ggggcuccgc cuacuauaug uaccuugauc      180

gcaacgacgc cggggaggcu auuucuuucc ccacaacccu gggaaugaau aaaugcuaua      240

uccagauuau ggaucucggc cacaugugug acgccacaau gagcuacgaa ugcccuaugc      300

uggaugaggg gguggaaccg gacgaugugg acuguuggug caacaccaca uccaccuggg      360

ucguguaugg aacgugucau cacaagaaag gcgaggcuag acggucucgc agagccguua      420

cccuucccag ccauuccaca cggaagcugc aaacccgcuc ucagacaugg cucgagagca      480

gagaauacac caagcaccug auccgggugg agaauuggau uuuucgcaac ccuggcuucg      540

cgcuggccgc ugccgcuauc gccuggcucc ugggguccag cacgucucag aaagucaucu      600

aucuugugau gauucugcuc aucgcgcccg ccuacuccau uagaugcauc ggaguuagca      660

aucgggauuu uguggaaggc augucugggg gaacaugggu cgacauugug cuggagcaug      720

gcgggugugu gaccgucaug gcucaggaua agccuacagu ugacaucgag cuugugacca      780

ccacaguguc caacauggcc gaaguccgca gcuacugcua ugaggcuucu auuuccgaua      840

uggccagcga cuccagaugu cccacgcaag gagaagccua ccuggacaaa cagucugaua      900

cccaguaugu gugcaagcgg acacucguug acagaggcug gggcaauggg uguggacugu      960

ucggcaaagg gagccuggug accugcgcua aguuugcgug cuccaagaaa augacaggaa     1020

agagcaucca gccugagaac cucgaauacc gcaucaugcu gucuguccac ggcucccaac     1080

acagcgggau gauugugaau gauaccggac augagacgga cgagaaccgg gccaaaguug     1140

aaaucacacc caauucuccc agagcugagg ccacccuugg cggcuucggg agccugggac     1200

ucgauuguga accucgcacc ggccuggacu uuuccgaucu uuauuaccug acaaugaaca     1260

auaagcacug gcucgugcau aaagaguggu uccacgacau uccccugccu uggcacgccg     1320

gggcugauac cggaacaccc cauuggaaca auaaggaagc ccuggucgag uuuaaagacg     1380

cgcacgcuaa gcggcagacg guggucgugc ucggcucuca ggagggggcc guucauaccg     1440

cccuggcugg cgcccuugaa gcugagaugg auggagccaa gggcagacug agcuccgggc     1500

accucaaaug ccgccugaag auggacaaac uucggcugaa gggagugagc uauucucucu     1560

guacagcggc cuucaccuuu acaaaaaucc cggcugaaac ccugcauggc acggucaccg     1620

uggagguuca guacgccggg acagacggac cuugcaaggu gcccgcucaa auggccgucg     1680

auaugcagac ccugacaccu gugggcagac ucauuaccgc gaaccccgug aucacggaau     1740

ccacagagaa cagcaagaug augcuggagc uugacccgcc uuucggggau ucuuacaucg     1800

uuauuggcgu cggagagaaa aagaucaccc accacuggca ucgcuccggc agcacaauug     1860

ggaaagccuu ugaagcuacc gugcggggag ccaagagaau ggcugugcug ggcgacaccg     1920

ccugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaaaggc auccaccaga     1980

uuuucggagc ugcguuuaag agccuguucg gcgggauguc cugguuuucu caaauccuua     2040

ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu ucccugaugu     2100

gucuugcccu gggaggcgug cucaucuucc ugagcaccgc uguuucugcc gacguggggu     2160

gcuccgucga uuuuagcaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6633
<211>  2509
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6633
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaaaaa gcggcgcggc gccgacacca gcguggggau cgucggacug cuccugacaa      120

cggcuauggc cgcggaaguu accagacggg gcuccgcuua cuauauguac cuugaucgca      180

acgacgccgg ggaggccauu ucuuucccca caacccuggg aaugaauaaa ugcuauaucc      240

agauuaugga ucucggccac augugugacg cuacaaugag cuacgaaugc ccuaugcugg      300

augagggggu ggaaccggac gauguggacu guuggugcaa caccacaucc accugggucg      360

uguauggaac gugucaucac aagaaaggcg aggccagacg gucucgcaga gcuguuaccc      420

uucccagcca uuccacacgg aagcugcaaa cccgcucuca gacauggcuc gagagcagag      480

aauacaccaa gcaccugauc cggguggaga auuggauuuu ucgcaacccu ggcuucgccc      540

uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaaa gucaucuauc      600

uugugaugau ucugcucauc gcccccgcgu acuccauuag augcaucgga guuagcaauc      660

gggauuuugu ggaaggcaug ucugggggaa caugggucga cauugugcug gagcauggcg      720

ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu gugaccacca      780

caguguccaa cauggcugaa guccgcagcu acugcuauga ggccucuauu uccgauaugg      840

cuagcgacuc cagauguccc acgcaaggag aagccuaccu ggacaaacag ucugauaccc      900

aguaugugug caagcggaca cucguugaca gaggcugggg caaugggugu ggacuguucg      960

gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caagaaaaug acaggaaaga     1020

gcauccagcc ugagaaccuc gaauaccgca ucaugcuguc uguccacggc ucccaacaca     1080

gcgggaugau ugugaaugau accggacaug agacggacga gaaccgggcg aaaguugaaa     1140

ucacacccaa uucucccaga gccgaggcua cccuuggcgg cuucgggagc cugggacucg     1200

auugugaacc ucgcaccggc cuggacuuuu ccgaucuuua uuaccugaca augaacaaua     1260

agcacuggcu cgugcauaaa gagugguucc acgacauucc ccugccuugg cacgccgggg     1320

ccgauaccgg aacaccccau uggaacaaua aggaagcucu ggucgaguuu aaagacgccc     1380

acgcgaagcg gcagacggug gucgugcucg gcucucagga gggggcuguu cauaccgccc     1440

uggccggcgc ucuugaagcc gagauggaug gagcuaaggg cagacugagc uccgggcacc     1500

ucaaaugccg ccugaagaug gacaaacuuc ggcugaaggg agugagcuau ucucucugua     1560

cagccgcguu caccuuuaca aaaaucccgg ccgaaacccu gcauggcacg gucaccgugg     1620

agguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug gcugucgaua     1680

ugcagacccu gacaccugug ggcagacuca uuaccgccaa ccccgugauc acggaaucca     1740

cagagaacag caagaugaug cuggagcuug acccgccuuu cggggauucu uacaucguua     1800

uuggcgucgg agagaaaaag aucacccacc acuggcaucg cuccggcagc acaauuggga     1860

aagcguuuga agccaccgug cggggagcua agagaauggc cgugcugggc gacaccgcuu     1920

gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc caccagauuu     1980

ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa auccuuaucg     2040

gaacacuccu gauguggcug ggccucaaca cgaagaaugg gagcauuucc cugauguguc     2100

uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac guggggugcu     2160

ccgucgauuu uagcaaauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2220

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2280

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2340

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2460

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2509


<210>  6634
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6634
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaaaaagag acggggcgcc gacaccagcg uggggaucgu cggacugcuc cugacaacgg      120

cuauggccgc ggaaguuacc cgcagaggcu ccgcuuacua uauguaccuu gaucggaacg      180

acgccgggga ggccauuucu uuccccacaa cccugggaau gaauaaaugc uauauccaga      240

uuauggaucu cggccacaug ugugacgcua caaugagcua cgaaugcccu augcuggaug      300

agggggugga accggacgau guggacuguu ggugcaacac cacauccacc ugggucgugu      360

auggaacgug ucaucacaag aaaggcgagg cccgcagauc ucggcgcgcu guuacccuuc      420

ccagccauuc cacaagaaag cugcaaaccc ggucucagac auggcucgag agccgcgaau      480

acaccaagca ccugaucaga guggagaauu ggauuuuucg gaacccuggc uucgcccugg      540

cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc aucuaucuug      600

ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu agcaauagag      660

auuuugugga aggcaugucu gggggaacau gggucgacau ugugcuggag cauggcgggu      720

gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug accaccacag      780

uguccaacau ggcugaaguc cggagcuacu gcuaugaggc cucuauuucc gauauggcua      840

gcgacucccg cugucccacg caaggagaag ccuaccugga caaacagucu gauacccagu      900

augugugcaa gagaacacuc guugaccggg gcuggggcaa ugggugugga cuguucggca      960

aagggagccu ggugaccugc gccaaguuug cuugcuccaa gaaaaugaca ggaaagagca     1020

uccagccuga gaaccucgaa uacagaauca ugcugucugu ccacggcucc caacacagcg     1080

ggaugauugu gaaugauacc ggacaugaga cggacgagaa ccgcgcgaaa guugaaauca     1140

cacccaauuc uccccgggcc gaggcuaccc uuggcggcuu cgggagccug ggacucgauu     1200

gugaaccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug aacaauaagc     1260

acuggcucgu gcauaaagag ugguuccacg acauuccccu gccuuggcac gccggggccg     1320

auaccggaac accccauugg aacaauaagg aagcucuggu cgaguuuaaa gacgcccacg     1380

cgaagcgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau accgcccugg     1440

ccggcgcucu ugaagccgag auggauggag cuaagggccg gcugagcucc gggcaccuca     1500

aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu cucuguacag     1560

ccgcguucac cuuuacaaaa aucccggccg aaacccugca uggcacgguc accguggagg     1620

uucaguacgc ugggacagac ggaccuugca aggugcccgc ccaaauggcu gucgauaugc     1680

agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg gaauccacag     1740

agaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac aucguuauug     1800

gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca auugggaaag     1860

cguuugaagc caccgugcgc ggagcuaagc ggauggccgu gcugggcgac accgcuuggg     1920

auuucggguc cgucggaggc gcccucaauu cucuggggaa aggcauccac cagauuuucg     1980

gagccgcuuu uaagagccug uucggcggga uguccugguu uucucaaauc cuuaucggaa     2040

cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug augugucuug     2100

cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacgug gggugcuccg     2160

ucgauuuuag caaaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6635
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6635
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagacg gggcgccgac accagcgugg ggaucgucgg acugcuccug acaacggcua      120

uggccgcgga aguuacccgc agaggcuccg cuuacuauau guaccuugau cggaacgacg      180

ccggggaggc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau auccagauua      240

uggaucucgg ccacaugugu gacgcuacaa ugagcuacga augcccuaug cuggaugagg      300

ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg gucguguaug      360

gaacguguca ucacaaaaag ggcgaggccc gcagaucucg gcgcgcuguu acccuuccca      420

gccauuccac aagaaaacug caaacccggu cucagacaug gcucgagagc cgcgaauaca      480

ccaagcaccu gaucagagug gagaauugga uuuuucggaa cccuggcuuc gcccuggcgg      540

ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaggucauc uaucuuguga      600

ugauucugcu caucgccccc gcguacucca uucgcugcau cggaguuagc aauagagauu      660

uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau ggcgggugug      720

ugaccgucau ggcccaggau aaaccuacag uugacaucga gcuugugacc accacagugu      780

ccaacauggc ugaaguccgg agcuacugcu augaggccuc uauuuccgau auggcuagcg      840

acucccgcug ucccacgcaa ggagaagccu accuggacaa gcagucugau acccaguaug      900

ugugcaaaag aacacucguu gaccggggcu ggggcaaugg guguggacug uucggcaagg      960

ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa gaugacagga aaaagcaucc     1020

agccugagaa ccucgaauac agaaucaugc ugucugucca cggcucccaa cacagcggga     1080

ugauugugaa ugauaccgga caugagacgg acgagaaccg cgcgaagguu gaaaucacac     1140

ccaauucucc ccgggccgag gcuacccuug gcggcuucgg gagccuggga cucgauugug     1200

aaccuagaac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac aauaaacacu     1260

ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc ggggccgaua     1320

ccggaacacc ccauuggaac aauaaagaag cucuggucga guuuaaggac gcccacgcga     1380

aacgccagac gguggucgug cucggcucuc aggagggggc uguucauacc gcccuggccg     1440

gcgcucuuga agccgagaug gauggagcua agggccggcu gagcuccggg caccucaagu     1500

gcagacugaa aauggacaaa cuucgccuga agggagugag cuauucucuc uguacagccg     1560

cguucaccuu uacaaagauc ccggccgaaa cccugcaugg cacggucacc guggagguuc     1620

aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc gauaugcaga     1680

cccugacacc ugugggccgg cucauuaccg ccaaccccgu gaucacggaa uccacagaga     1740

acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc guuauuggcg     1800

ucggagaaaa gaaaaucacc caccacuggc auagauccgg cagcacaauu gggaaggcgu     1860

uugaggccac cgugcgcgga gcuaaacgga uggccgugcu gggcgacacc gcuugggauu     1920

ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag auuuucggag     1980

ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu aucggaacac     2040

uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug ugucuugcgc     2100

ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacgugggg ugcuccgucg     2160

auuuuagcaa gugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  6636
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6636
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagacgggg cgccgacacc agcgugggga ucgucggacu gcuccugaca acggcuaugg      120

ccgcggaggu uacccgcaga ggcuccgcuu acuauaugua ccuugaucgg aacgacgccg      180

gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc cagauuaugg      240

aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug gaugaagggg      300

uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc guguauggaa      360

cgugucauca caaaaagggc gaagcccgca gaucucggcg cgcuguuacc cuucccagcc      420

auuccacaag aaaacugcaa acccggucuc agacauggcu cgagagccgc gaguacacca      480

agcaccugau cagaguggaa aauuggauuu uucggaaccc uggcuucgcc cuggcggccg      540

cugccaucgc uuggcuccug ggguccagca cgucucagaa ggucaucuau cuugugauga      600

uucugcucau cgcccccgcg uacuccauuc gcugcaucgg aguuagcaau agagauuuug      660

uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc ggguguguga      720

ccgucauggc ccaggauaaa ccuacaguug acaucgagcu ugugaccacc acagugucca      780

acauggcuga gguccggagc uacugcuaug aagccucuau uuccgauaug gcuagcgacu      840

cccgcugucc cacgcaagga gaggccuacc uggacaagca gucugauacc caguaugugu      900

gcaaaagaac acucguugac cggggcuggg gcaaugggug uggacuguuc ggcaagggga      960

gccuggugac cugcgccaaa uuugcuugcu ccaagaagau gacaggaaaa agcauccagc     1020

cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac agcgggauga     1080

uugugaauga uaccggacau gaaacggacg agaaccgcgc gaagguugag aucacaccca     1140

auucuccccg ggccgaagcu acccuuggcg gcuucgggag ccugggacuc gauugugagc     1200

cuagaaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau aaacacuggc     1260

ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg gccgauaccg     1320

gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc cacgcgaaac     1380

gccagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc cuggccggcg     1440

cucuugaggc cgaaauggau ggagcuaagg gccggcugag cuccgggcac cucaagugca     1500

gacugaaaau ggacaaacuu cgccugaagg gagugagcua uucucucugu acagccgcgu     1560

ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug gaaguucagu     1620

acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau augcagaccc     1680

ugacaccugu gggccggcuc auuaccgcca accccgugau cacggagucc acagaaaaca     1740

gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu auuggcgucg     1800

gagagaagaa aaucacccac cacuggcaua gauccggcag cacaauuggg aaggcguuug     1860

aagccaccgu gcgcggagcu aaacggaugg ccgugcuggg cgacaccgcu ugggauuucg     1920

gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu uucggagccg     1980

cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc ggaacacucc     2040

ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu cuugcgcugg     2100

gaggcgugcu caucuuccug agcaccgccg uuucugcuga cguggggugc uccgucgauu     2160

uuagcaagug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  6637
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6637
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gacggggcgc cgacaccagc guggggaucg ucggacugcu ccugacaacg gcuauggccg      120

cggagguuac ccgcagaggc uccgcuuacu auauguaccu ugaucggaac gacgccgggg      180

aagccauuuc uuuccccaca acccugggaa ugaauaaaug cuauauccag auuauggauc      240

ucggccacau gugugacgcu acaaugagcu acgagugccc uaugcuggau gaaggggugg      300

agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug uauggaacgu      360

gucaucacaa gaaaggcgaa gcccgcagau cucggcgcgc uguuacccuu cccagccauu      420

ccacaagaaa gcugcaaacc cggucucaga cauggcucga gagccgcgag uacaccaaac      480

accugaucag aguggaaaau uggauuuuuc ggaacccugg cuucgcccug gcggccgcug      540

ccaucgcuug gcuccugggg uccagcacgu cucagaaggu caucuaucuu gugaugauuc      600

ugcucaucgc ccccgcguac uccauucgcu gcaucggagu uagcaauaga gauuuugugg      660

agggcauguc ugggggaaca ugggucgaca uugugcugga acauggcggg ugugugaccg      720

ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca guguccaaca      780

uggcugaggu ccggagcuac ugcuaugaag ccucuauuuc cgauauggcu agcgacuccc      840

gcugucccac gcaaggagag gccuaccugg acaaacaguc ugauacccag uaugugugca      900

agagaacacu cguugaccgg ggcuggggca augggugugg acuguucggc aaagggagcc      960

uggugaccug cgccaaguuu gcuugcucca aaaagaugac aggaaagagc auccagccug     1020

aaaaccucga guacagaauc augcugucug uccacggcuc ccaacacagc gggaugauug     1080

ugaaugauac cggacaugaa acggacgaga accgcgcgaa aguugagauc acacccaauu     1140

cuccccgggc cgaagcuacc cuuggcggcu ucgggagccu gggacucgau ugugagccua     1200

gaaccggccu ggacuuuucc gaucuuuauu accugacaau gaacaauaag cacuggcucg     1260

ugcauaaaga augguuccac gacauucccc ugccuuggca cgccggggcc gauaccggaa     1320

caccccauug gaacaauaag gaggcucugg ucgaauuuaa agacgcccac gcgaagcgcc     1380

agacgguggu cgugcucggc ucucaggagg gggcuguuca uaccgcccug gccggcgcuc     1440

uugaggccga aauggaugga gcuaaaggcc ggcugagcuc cgggcaccuc aagugcagac     1500

ugaagaugga caaacuucgc cugaagggag ugagcuauuc ucucuguaca gccgcguuca     1560

ccuuuacaaa aaucccggcc gagacccugc auggcacggu caccguggaa guucaguacg     1620

cugggacaga cggaccuugc aaggugcccg cccaaauggc ugucgauaug cagacccuga     1680

caccuguggg ccggcucauu accgccaacc ccgugaucac ggaguccaca gaaaacagca     1740

aaaugaugcu ggagcuugac ccgccuuucg gggauucuua caucguuauu ggcgucggag     1800

agaagaagau cacccaccac uggcauagau ccggcagcac aauugggaaa gcguuugaag     1860

ccaccgugcg cggagcuaag cggauggccg ugcugggcga caccgcuugg gauuucgggu     1920

ccgucggagg cgcccucaau ucucugggga aaggcaucca ccagauuuuc ggagccgcuu     1980

uuaagagccu guucggcggg auguccuggu uuucucaaau ccuuaucgga acacuccuga     2040

uguggcuggg ccucaacacg aaaaauggga gcauuucccu gaugugucuu gcgcugggag     2100

gcgugcucau cuuccugagc accgccguuu cugcugacgu ggggugcucc gucgauuuua     2160

gcaagugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6638
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6638
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgccga caccagcgug gggaucgucg gacugcuccu gacaacggcu auggccgcgg      120

agguuacccg cagaggcucc gcuuacuaua uguaccuuga ucggaacgac gccggggaag      180

ccauuucuuu ccccacaacc cugggaauga auaagugcua uauccagauu auggaucucg      240

gccacaugug ugacgcuaca augagcuacg agugcccuau gcuggaugaa gggguggagc      300

cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau ggaacguguc      360

aucacaaaaa gggcgaagcc cgcagaucuc ggcgcgcugu uacccuuccc agccauucca      420

caagaaaacu gcaaacccgg ucucagacau ggcucgagag ccgcgaguac accaagcacc      480

ugaucagagu ggaaaauugg auuuuucgga acccuggcuu cgcccuggcg gccgcugcca      540

ucgcuuggcu ccuggggucc agcacgucuc agaaagucau cuaucuugug augauucugc      600

ucaucgcccc cgcguacucc auucgcugca ucggaguuag caauagagau uuuguggagg      660

gcaugucugg gggaacaugg gucgacauug ugcuggaaca uggcgggugu gugaccguca      720

uggcccagga uaagccuaca guugacaucg agcuugugac caccacagug uccaacaugg      780

cugagguccg gagcuacugc uaugaagccu cuauuuccga uauggcuagc gacucccgcu      840

gucccacgca aggagaggcc uaccuggaca agcagucuga uacccaguau gugugcaaaa      900

gaacacucgu ugaccggggc uggggcaaug gguguggacu guucggcaag gggagccugg      960

ugaccugcgc caaauuugcu ugcuccaaga aaaugacagg aaagagcauc cagccugaaa     1020

accucgagua cagaaucaug cugucugucc acggcuccca acacagcggg augauuguga     1080

augauaccgg acaugaaacg gacgagaacc gcgcgaaggu ugagaucaca cccaauucuc     1140

cccgggccga agcuacccuu ggcggcuucg ggagccuggg acucgauugu gagccuagaa     1200

ccggccugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac uggcucgugc     1260

auaaggaaug guuccacgac auuccccugc cuuggcacgc cggggccgau accggaacac     1320

cccauuggaa caauaaagag gcucuggucg aauuuaagga cgcccacgcg aaacgccaga     1380

cgguggucgu gcucggcucu caggaggggg cuguucauac cgcccuggcc ggcgcucuug     1440

aggccgaaau ggauggagcu aagggccggc ugagcuccgg gcaccucaaa ugcagacuga     1500

agauggacaa acuucgccug aagggaguga gcuauucucu cuguacagcc gcguucaccu     1560

uuacaaagau cccggccgag acccugcaug gcacggucac cguggaaguu caguacgcug     1620

ggacagacgg accuugcaaa gugcccgccc aaauggcugu cgauaugcag acccugacac     1680

cugugggccg gcucauuacc gccaaccccg ugaucacgga guccacagaa aacagcaaga     1740

ugaugcugga gcuugacccg ccuuucgggg auucuuacau cguuauuggc gucggagaga     1800

aaaagaucac ccaccacugg cauagauccg gcagcacaau ugggaaggcg uuugaagcca     1860

ccgugcgcgg agcuaaacgg auggccgugc ugggcgacac cgcuugggau uucggguccg     1920

ucggaggcgc ccucaauucu cuggggaagg gcauccacca gauuuucgga gccgcuuuua     1980

aaagccuguu cggcgggaug uccugguuuu cucaaauccu uaucggaaca cuccugaugu     2040

ggcugggccu caacacgaag aaugggagca uuucccugau gugucuugcg cugggaggcg     2100

ugcucaucuu ccugagcacc gccguuucug cugacguggg gugcuccguc gauuuuagca     2160

aaugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6639
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6639
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug gccgcggagg      120

uuacccggcg cggcuccgcu uacuauaugu accuugauag aaacgacgcc ggggaagcca      180

uuucuuuccc cacaacccug ggaaugaaua agugcuauau ccagauuaug gaucucggcc      240

acauguguga cgcuacaaug agcuacgagu gcccuaugcu ggaugaaggg guggagccgg      300

acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga acgugucauc      360

acaaaaaggg cgaagcccgg cgcucuagac gggcuguuac ccuucccagc cauuccacac      420

gcaaacugca aaccagaucu cagacauggc ucgagagccg ggaguacacc aagcaccuga      480

uccgcgugga aaauuggauu uuuagaaacc cuggcuucgc ccuggcggcc gcugccaucg      540

cuuggcuccu gggguccagc acgucucaga aagucaucua ucuugugaug auucugcuca      600

ucgcccccgc guacuccauu cggugcaucg gaguuagcaa ucgcgauuuu guggagggca      660

ugucuggggg aacauggguc gacauugugc uggaacaugg cgggugugug accgucaugg      720

cccaggauaa gccuacaguu gacaucgagc uugugaccac cacagugucc aacauggcug      780

aggucagaag cuacugcuau gaagccucua uuuccgauau ggcuagcgac ucccgguguc      840

ccacgcaagg agaggccuac cuggacaagc agucugauac ccaguaugug ugcaaacgca      900

cacucguuga cagaggcugg ggcaaugggu guggacuguu cggcaagggg agccugguga      960

ccugcgccaa auuugcuugc uccaagaaaa ugacaggaaa gagcauccag ccugaaaacc     1020

ucgaguaccg gaucaugcug ucuguccacg gcucccaaca cagcgggaug auugugaaug     1080

auaccggaca ugaaacggac gagaacagag cgaagguuga gaucacaccc aauucucccc     1140

gcgccgaagc uacccuuggc ggcuucggga gccugggacu cgauugugag ccucggaccg     1200

gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaaacacugg cucgugcaua     1260

aggaaugguu ccacgacauu ccccugccuu ggcacgccgg ggccgauacc ggaacacccc     1320

auuggaacaa uaaagaggcu cuggucgaau uuaaggacgc ccacgcgaaa agacagacgg     1380

uggucgugcu cggcucucag gagggggcug uucauaccgc ccuggccggc gcucuugagg     1440

ccgaaaugga uggagcuaag ggccgccuga gcuccgggca ccucaaaugc cggcugaaga     1500

uggacaaacu uagacugaag ggagugagcu auucucucug uacagccgcg uucaccuuua     1560

caaagauccc ggccgagacc cugcauggca cggucaccgu ggaaguucag uacgcuggga     1620

cagacggacc uugcaaagug cccgcccaaa uggcugucga uaugcagacc cugacaccug     1680

ugggccgccu cauuaccgcc aaccccguga ucacggaguc cacagaaaac agcaagauga     1740

ugcuggagcu ugacccgccu uucggggauu cuuacaucgu uauuggcguc ggagagaaaa     1800

agaucaccca ccacuggcau cgguccggca gcacaauugg gaaggcguuu gaagccaccg     1860

ugagaggagc uaaacgcaug gccgugcugg gcgacaccgc uugggauuuc ggguccgucg     1920

gaggcgcccu caauucucug gggaagggca uccaccagau uuucggagcc gcuuuuaaaa     1980

gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc cugauguggc     2040

ugggccucaa cacgaagaau gggagcauuu cccugaugug ucuugcgcug ggaggcgugc     2100

ucaucuuccu gagcaccgcc guuucugcug acguggggug cuccgucgau uuuagcaaau     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6640
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6640
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gucggacugc uccugacaac ggcuauggcc gcggagguua      120

ccagacgggg cuccgcuuac uauauguacc uugaucgcaa cgacgccggg gaagccauuu      180

cuuuccccac aacccuggga augaauaagu gcuauaucca gauuauggau cucggccaca      240

ugugugacgc uacaaugagc uacgagugcc cuaugcugga ugaaggggug gagccggacg      300

auguggacug uuggugcaac accacaucca ccugggucgu guauggaacg ugucaucaca      360

aaaagggcga agccagacgg ucucgcagag cuguuacccu ucccagccau uccacacgga      420

aacugcaaac ccgcucucag acauggcucg agagcagaga guacaccaag caccugaucc      480

ggguggaaaa uuggauuuuu cgcaacccug gcuucgcccu ggcggccgcu gccaucgcuu      540

ggcuccuggg guccagcacg ucucagaaag ucaucuaucu ugugaugauu cugcucaucg      600

cccccgcgua cuccauuaga ugcaucggag uuagcaaucg ggauuuugug gagggcaugu      660

cugggggaac augggucgac auugugcugg aacauggcgg gugugugacc gucauggccc      720

aggauaagcc uacaguugac aucgagcuug ugaccaccac aguguccaac auggcugagg      780

uccgcagcua cugcuaugaa gccucuauuu ccgauauggc uagcgacucc agauguccca      840

cgcaaggaga ggccuaccug gacaagcagu cugauaccca guaugugugc aaacggacac      900

ucguugaccg cggcuggggc aaugggugug gacuguucgg caaggggagc cuggugaccu      960

gcgccaaauu ugcuugcucc aagaaaauga caggaaagag cauccagccu gaaaaccucg     1020

aguacagaau caugcugucu guccacggcu cccaacacag cgggaugauu gugaaugaua     1080

ccggacauga aacggacgag aaccgggcga agguugagau cacacccaau ucucccagag     1140

ccgaagcuac ccuuggcggc uucgggagcc ugggacucga uugugagccu cgcaccggcc     1200

uggacuuuuc cgaucuuuau uaccugacaa ugaacaauaa acacuggcuc gugcauaagg     1260

aaugguucca cgacauuccc cugccuuggc acgccggggc cgauaccgga acaccccauu     1320

ggaacaauaa agaggcucug gucgaauuua aggacgccca cgcgaaacgg cagacggugg     1380

ucgugcucgg cucucaggag ggggcuguuc auaccgcccu ggccggcgcu cuugaggccg     1440

aaauggaugg agcuaagggc agacugagcu ccgggcaccu caaaugccgc cugaagaugg     1500

acaaacuucg gcugaaggga gugagcuauu cucucuguac agccgcguuc accuuuacaa     1560

agaucccggc cgagacccug cauggcacgg ucaccgugga aguucaguac gcugggacag     1620

acggaccuug caaagugccc gcccaaaugg cugucgauau gcagacccug acaccugugg     1680

gcagacucau uaccgccaac cccgugauca cggaguccac agaaaacagc aagaugaugc     1740

uggagcuuga cccgccuuuc ggggauucuu acaucguuau uggcgucgga gagaaaaaga     1800

ucacccacca cuggcaucgc uccggcagca caauugggaa ggcguuugaa gccaccgugc     1860

ggggagcuaa aagaauggcc gugcugggcg acaccgcuug ggauuucggg uccgucggag     1920

gcgcccucaa uucucugggg aagggcaucc accagauuuu cggagccgcu uuuaaaagcc     1980

uguucggcgg gauguccugg uuuucucaaa uccuuaucgg aacacuccug auguggcugg     2040

gccucaacac gaagaauggg agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca     2100

ucuuccugag caccgccguu ucugcugacg uggggugcuc cgucgauuuu agcaaaugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6641
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6641
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucguc gggcugcucc ugacaacggc uauggccgcg gagguuacca      120

gacggggauc cgcuuacuau auguaccuug aucgcaacga cgccggcgaa gccauuucuu      180

uccccacaac ccuggggaug aauaagugcu auauccagau uauggaucuc ggacacaugu      240

gugacgcuac aaugagcuac gagugcccua ugcuggauga aggcguggag ccggacgaug      300

uggacuguug gugcaacacc acauccaccu gggucgugua ugggacgugu caucacaaaa      360

agggagaagc cagacggucu cgcagagcug uuacccuucc cagccauucc acacggaaac      420

ugcaaacccg cucucagaca uggcucgaga gcagagagua caccaagcac cugauccggg      480

uggaaaauug gauuuuucgc aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc      540

uccugggcuc cagcacgucu cagaaaguca ucuaucuugu gaugauucug cucaucgccc      600

ccgcguacuc cauuagaugc aucgggguua gcaaucggga uuuuguggag ggaaugucug      660

gcgggacaug ggucgacauu gugcuggaac auggaggcug ugugaccguc auggcccagg      720

auaagccuac aguugacauc gagcuuguga ccaccacagu guccaacaug gcugaggucc      780

gcagcuacug cuaugaagcc ucuauuuccg auauggcuag cgacuccaga ugucccacgc      840

aaggggaggc cuaccuggac aagcagucug auacccagua ugugugcaaa cggacacucg      900

uugaccgcgg auggggcaau ggcugugggc uguucggaaa gggcagccug gugaccugcg      960

ccaaauuugc uugcuccaag aaaaugacag gaaagagcau ccagccugaa aaccucgagu     1020

acagaaucau gcugucuguc cacggguccc aacacagcgg caugauugug aaugauaccg     1080

ggcaugaaac ggacgagaac cgggcgaagg uugagaucac acccaauucu cccagagccg     1140

aagcuacccu uggaggcuuc ggcagccugg ggcucgauug ugagccucgc accggacugg     1200

acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug cauaaggaau     1260

gguuccacga cauuccccug ccuuggcacg ccggcgccga uaccgggaca ccccauugga     1320

acaauaaaga ggcucugguc gaauuuaagg acgcccacgc gaaacggcag acgguggucg     1380

ugcucggauc ucaggagggc gcuguucaua ccgcccuggc cggggcucuu gaggccgaaa     1440

uggauggcgc uaagggaaga cugagcuccg gccaccucaa augccgccug aagauggaca     1500

agcugcggcu gaaaggggug agcuauucuc ucuguacagc cgcguucacc uuuacaaaga     1560

ucccggccga gacccugcau ggaacgguca ccguggaagu ucaguacgcu ggcacagacg     1620

ggccuugcaa agugcccgcc caaauggcug ucgauaugca gacccugaca ccugugggaa     1680

gacucauuac cgccaacccc gugaucacgg aguccacaga aaacagcaag augaugcugg     1740

agcuugaccc gccuuucggc gauucuuaca ucguuauugg ggucggcgag aaaaagauca     1800

cccaccacug gcaucgcucc ggaagcacaa uuggcaaggc guuugaagcc accgugagag     1860

gggcuaaacg gauggccgug cugggagaca ccgcuuggga uuucggcucc gucgggggag     1920

cccucaauuc ucugggcaag gggauccacc agauuuucgg cgccgcuuuu aaaagccugu     1980

ucggaggcau guccugguuu ucucaaaucc uuaucgggac acuccugaug uggcugggac     2040

ucaacacgaa gaauggcagc auuucccuga ugugucuugc gcuuggggga gugcucaucu     2100

uccugagcac cgccguuucu gcugacgugg gcugcuccgu cgauuuuagc aaaugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6642
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6642
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgucggg cugcuccuga caacggccau ggcugccgag guuaccagac      120

ggggauccgc guacuauaug uaccuugauc gcaacgacgc uggcgaagcc auuucuuucc      180

ccacaacccu ggggaugaau aagugcuaua uccagauuau ggaucucgga cacaugugug      240

acgccacaau gagcuacgag ugcccuaugc uggaugaagg cguggagccg gacgaugugg      300

acuguuggug caacaccaca uccaccuggg ucguguaugg gacgugucau cacaaaaagg      360

gagaagcuag acggucucgc agagccguua cccuucccag ccauuccaca cggaaacugc      420

aaacccgcuc ucagacaugg cucgagagca gagaguacac caagcaccug auccgggugg      480

aaaauuggau uuuucgcaac ccuggcuucg cucuggccgc ggccgcuauc gccuggcucc      540

ugggcuccag cacgucucag aaagucaucu aucuugugau gauucugcuc aucgcucccg      600

ccuacuccau uagaugcauc gggguuagca aucgggauuu uguggaggga augucuggcg      660

ggacaugggu cgacauugug cuggaacaug gaggcugugu gaccgucaug gcgcaggaua      720

agccuacagu ugacaucgag cuugugacca ccacaguguc caacauggcc gagguccgca      780

gcuacugcua ugaagcuagc auuuccgaua uggccagcga cuccagaugu cccacgcaag      840

gggaggcuua ccuggacaag cagucugaua cccaguaugu gugcaaacgg acacucguug      900

accgcggaug gggcaauggc ugugggcugu ucggaaaggg cagccuggug accugcgcca      960

aauuugccug cuccaagaaa augacaggga agucuaucca gccugaaaac cucgaguaca     1020

gaaucaugcu gucuguccac ggaucccaac acagcggcau gauugugaau gauaccgggc     1080

augaaacgga cgagaaccgg gcuaagguug agaucacacc caauucuccc agagcggaag     1140

ccacccuugg aggcuucggc agccuggggc ucgauuguga gccucgcacc ggacuggacu     1200

uuuccgaucu uuauuaccug acaaugaaca auaaacacug gcucgugcau aaggaauggu     1260

uccacgacau uccccugccu uggcacgcug gcgccgauac cgggacaccc cauuggaaca     1320

auaaagaggc ccuggucgaa uuuaaggacg cucacgccaa acggcagacg guggucgugc     1380

ucggaucuca ggagggcgcg guucauaccg cucuggccgg ggcccuugag gcugaaaugg     1440

auggcgccaa gggaagacug agcuccggcc accucaaaug ccgccugaag auggacaagc     1500

ugcggcugaa aggggugagc uauucucucu guacagcugc cuucaccuuu acaaagaucc     1560

cggcggagac ccugcaugga acggucaccg uggaaguuca guacgccggc acagacgggc     1620

cuugcaaagu gcccgcucaa auggccgucg auaugcagac ccugacaccu gugggaagac     1680

ucauuaccgc uaaccccgug aucacggagu ccacagaaaa cagcaagaug augcuggagc     1740

uugacccgcc uuucggcgau ucuuacaucg uuauuggggu cggcgagaaa aagaucaccc     1800

accacuggca ucgcuccgga agcacaauug gcaaggccuu ugaagcgacc gugagagggg     1860

ccaaacggau ggcugugcug ggagacaccg ccugggauuu cggcuccguc gggggagcuc     1920

ucaauucucu gggcaagggg auccaccaga uuuucggcgc cgccuuuaaa agccuguucg     1980

gaggcauguc cugguuuucu caaauccuua ucgggacacu ccugaugugg cugggacuca     2040

acacgaagaa uggcagcauu ucccugaugu gucuugcucu ugggggagug cucaucuucc     2100

ugagcaccgc gguuucugcc gacgugggcu gcuccgucga uuuuagcaaa ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6643
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6643
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccgau gugggcugcu ccgucgacuu uagcaaauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6644
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6644
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgcgguu accagacggg      120

gauccgcuua cuauauguac cuugaccgca acgaugccgg cgaggccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

cuacaaugag cuacgaaugc ccuaugcugg acgagggcgu ggaaccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aggccagacg gucucgcaga gcuguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gaaagcagag aguacaccaa gcaccugauc cggguggaga      480

auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcccccgcgu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggaaggaaug ucuggcggga      660

caugggucga uauugugcug gagcauggag gcugugugac cgucauggcc caggacaagc      720

cuacaguuga uaucgaacuu gugaccacca caguguccaa cauggcugag guccgcagcu      780

acugcuauga ggccucuauu uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aagccuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugcuugcuc caagaaaaug acagggaagu cuauccagcc ugagaaccuc gaauacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

agacggauga aaaccgggcg aagguugaga ucacaccgaa uagccccaga gccgaggcua     1140

cccuuggagg cuucggcagc cuggggcucg acugugaacc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gagugguucc     1260

acgauauucc ccugccuugg cacgccggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaagcucu ggucgaguuu aaggaugccc acgcgaaacg gcagacggug gucgugcucg     1380

gaucucagga aggcgcuguu cauaccgccc uggccggggc ucuugaggcc gagauggacg     1440

gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca aagaucccgg     1560

ccgaaacccu gcauggaacg gucaccgugg agguucagua cgcuggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcugucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgccaa ccccgugauc acggaaucca cagagaacag caagaugaug cuggaacuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggcguuuga ggccaccgug agaggggcua     1860

aacggauggc cgugcuggga gauaccgcuu gggacuucgg cuccgucggg ggagcccuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc aucuuccuga     2100

gcaccgccgu uucugcugau gugggcugcu ccgucgacuu uagcaaauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6645
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6645
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug gccgcggcug      120

uuacccggcg cggcuccgcc uacuauaugu accuugauag aaacgacgcc ggggaggcua      180

uuucuuuccc cacaacccug ggaaugaaua agugcuauau ccagauuaug gaucucggcc      240

acauguguga cgccacaaug agcuacgaau gcccuaugcu ggaugagggg guggaaccgg      300

acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga acgugucauc      360

acaaaaaggg cgaggcucgg cgcucuagac gggccguuac ccuucccagc cauuccacac      420

gcaaacugca aaccagaucu cagacauggc ucgaaagccg ggaguacacc aagcaccuga      480

uccgcgugga gaauuggauu uuuagaaacc cuggcuucgc gcuggccgcu gccgcuaucg      540

ccuggcuccu gggguccagc acgucucaga aagucaucua ucuugugaug auucugcuca      600

ucgcgcccgc cuacuccauu cggugcaucg gaguuagcaa ucgcgauuuu guggaaggca      660

ugucuggggg aacauggguc gacauugugc uggagcaugg cgggugugug accgucaugg      720

cucaggauaa gccuacaguu gacaucgaac uugugaccac cacagugucc aacauggccg      780

aggucagaag cuacugcuau gaggcuucua uuuccgauau ggccagcgac ucccgguguc      840

ccacgcaagg agaagccuac cuggacaagc agucugauac ccaguaugug ugcaaacgca      900

cacucguuga cagaggcugg ggcaaugggu guggacuguu cggcaagggg agccugguga      960

ccugcgcuaa auuugcgugc uccaagaaaa ugacaggaaa gagcauccag ccugagaacc     1020

ucgaauaccg gaucaugcug ucuguccacg gcucccaaca cagcgggaug auugugaaug     1080

auaccggaca ugagacggac gaaaacagag ccaagguuga gaucacaccc aauucucccc     1140

gcgcugaggc cacccuuggc ggcuucggga gccugggacu cgauugugaa ccucggaccg     1200

gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaaacacugg cucgugcaua     1260

aggagugguu ccacgacauu ccccugccuu ggcacgccgg ggcugauacc ggaacacccc     1320

auuggaacaa uaaagaagcc cuggucgagu uuaaggacgc gcacgcuaaa agacagacgg     1380

uggucgugcu cggcucucag gaaggggccg uucauaccgc ccuggcuggc gcccuugagg     1440

cugagaugga uggagccaag ggccgccuga gcuccgggca ccucaaaugc cggcugaaga     1500

uggacaaacu uagacugaag ggagugagcu auucucucug uacagcggcc uucaccuuua     1560

caaagauccc ggcugaaacc cugcauggca cggucaccgu ggagguucag uacgccggga     1620

cagacggacc uugcaaagug cccgcucaaa uggccgucga uaugcagacc cugacaccug     1680

ugggccgccu cauuaccgcg aaccccguga ucacggaauc cacagagaac agcaagauga     1740

ugcuggaacu ugacccgccu uucggggauu cuuacaucgu uauuggcguc ggagagaaaa     1800

agaucaccca ccacuggcau cgguccggca gcacaauugg gaaggccuuu gaggcuaccg     1860

ugagaggagc caaacgcaug gcugugcugg gcgacaccgc cugggauuuc ggguccgucg     1920

gaggcgcccu caauucucug gggaagggca uccaccagau uuucggagcu gcguuuaaaa     1980

gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc cugauguggc     2040

ugggccucaa cacgaagaau gggagcauuu cccugaugug ucuugcccug ggaggcgugc     2100

ucaucuuccu gagcaccgcu guuucugccg acguggggug cuccgucgau uuuagcaaau     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6646
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6646
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc ccggaagcgc agaucucacg acgugcucac cguccaguuc cugauccuug      120

ggaugcugcu caugacagga ggcguuacgc ggcgcgggag cgccuacuau auguaccugg      180

auagaaacga cgcuggagag gccauuuccu uucccaccac acuuggcaug aauaaaugcu      240

auauccaaau uauggaucug gggcauaugu gugacgcgac caugucuuac gaaugcccua      300

ugcucgauga gggaguggaa ccggacgaug uggacuguug gugcaacaca accagcacau      360

gggucgugua uggcaccugu caccauaaga aaggcgaggc ucggcgcucc agacgggccg      420

uuacgcugcc cucucacagc acccgcaagc ugcagacaag aucccagacc uggcucgaaa      480

gccgggagua cacaaaacau cugauccgcg uggagaauug gauuuucaga aacccugggu      540

uugcccuugc ugccgcugcc aucgcguggc ugcucggauc uuccaccagc cagaagguca      600

ucuaucuggu gaugauucuu cugaucgccc ccgcuuacuc uauucggugc aucggcguuu      660

ccaaucgcga uuucguggaa gggaugagcg gaggcacgug ggucgacauu gugcucgagc      720

acgggggaug ugugacaguc auggcccaag auaagccuac cguugacauc gaacugguga      780

caaccaccgu gucuaacaug gcugagguca gauccuacug cuaugaggcc agcauuuccg      840

auauggcguc ugacagccgg ugucccacac agggcgaagc cuaccuggac aaacaguccg      900

auacgcagua ugugugcaag agaacccucg uugaccgcgg cugggggaau ggauguggcc      960

uguuugggaa aggaagccuu gugacaugcg cuaaguucgc cugcucuaaa aagaugaccg     1020

gcaaguccau ccaaccugag aaccuggaau accggaucau gcucagcguc cacgggucuc     1080

agcauagcgg aaugauugug aaugauacag gccacgagac cgacgaaaac agagcuaaag     1140

uugagaucac gcccaauucc ccgcgcgccg aggccacacu gggcggguuu ggaucucuug     1200

gccuggauug ugaaccucgg accgggcucg acuucagcga ucuguauuac cugaccauga     1260

acaauaagca uuggcucgug cacaaagagu gguuucacga cauuccccug ccuuggcaug     1320

cuggagcgga uacaggcaca ccccacugga acaauaagga agcccuuguc gaguucaaag     1380

acgcucaugc caagagacag acaguggucg ugcugggguc ccaggaaggc gccguucaca     1440

cggcucucgc cggagcgcug gaggcugaga uggauggcgc caaagggcgc cuuagcucug     1500

gacaucugaa gugccggcuc aagauggaca aacugagacu gaagggcgug uccuauagcc     1560

ucuguaccgc cgcuuuuaca uucaccaaaa ucccggccga aacacugcac gggaccguca     1620

cgguggaggu ucaauacgcu ggaaccgacg gcccuugcaa ggugcccgcc cagauggcgg     1680

ucgauaugca gacacuuacc ccuguggggc gccugauuac agccaacccc gugaucaccg     1740

aaucuacgga gaacuccaaa augaugcucg aacuggaucc cccuuuuggc gacagcuaca     1800

ucguuauugg agucggcgag aagaagauca cacaccauug gcaccggucc gggucuacca     1860

uuggaaaggc uuucgaggcc acagugagag gcgcuaaacg cauggccgug cugggggaca     1920

ccgcguggga uuucggaagc gucggcgggg cccuuaauuc ccucggcaaa ggaauccauc     1980

aaauuuuugg cgcugccuuc aagucucugu uugggggaau gagcugguuc ucccagaucc     2040

ugaucggcac ccuccugaug uggcuugggc ugaacaccaa aaauggaagc auuucucuca     2100

ugugucuggc ucugggcggg gugcuuaucu uucucuccac ggccguuagc gccgacgugg     2160

gcugcucugu cgauuucucc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6647
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6647
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc ccggaagcgc agaucucacg acgugcucac cguccaguuc cugauccuug      120

ggaugcugcu caugacagga gccguuacgc ggcgcggcag cgcuuacuau auguaccugg      180

auagaaacga cgccggggag gcgauuuccu uucccaccac acuuggaaug aauaaaugcu      240

auauccaaau uauggaucug ggccauaugu gugacgcuac caugucuuac gaaugcccua      300

ugcucgauga ggggguggaa ccggacgaug uggacuguug gugcaacaca accagcacau      360

gggucgugua uggaaccugu caccauaaga aaggcgaggc ccggcgcucc agacgggccg      420

uuacgcugcc cucucacagc acccgcaagc ugcagacaag aucccagacc uggcucgaaa      480

gccgggagua cacaaaacau cugauccgcg uggagaauug gauuuucaga aacccuggcu      540

uugcucuugc cgcugccgcg aucgccuggc ugcucggguc uuccaccagc cagaagguca      600

ucuaucuggu gaugauucuu cugaucgcuc ccgccuacuc uauucggugc aucggaguuu      660

ccaaucgcga uuucguggaa ggcaugagcg ggggaacgug ggucgacauu gugcucgagc      720

acggcgggug ugugacaguc auggcucaag auaagccuac cguugacauc gaacugguga      780

caaccaccgu gucuaacaug gccgagguca gauccuacug cuaugaggcg agcauuuccg      840

auauggccuc ugacagccgg ugucccacac agggagaggc uuaccuggac aaacaguccg      900

auacgcagua ugugugcaag agaacccucg uugaccgcgg cuggggcaau ggguguggac      960

uguuuggcaa agggagccuu gugacaugcg ccaaguucgc uugcucuaaa aagaugaccg     1020

gaaaguccau ccaaccugaa aaccuggaau accggaucau gcucagcguc cacggcucuc     1080

agcauagcgg gaugauugug aaugauacag gacacgagac cgacgaaaac agagccaaag     1140

uugagaucac gcccaauucc ccgcgcgccg aggcuacacu gggcggcuuu gggucucuug     1200

gacuggauug ugaaccucgg accggccucg acuucagcga ucuguauuac cugaccauga     1260

acaauaagca uuggcucgug cacaaagagu gguuucacga cauuccccug ccuuggcaug     1320

cgggggccga uacaggaaca ccccacugga acaauaagga agcucuuguc gaguucaaag     1380

acgcccaugc caagagacag acaguggucg ugcugggcuc ccaggaaggg gcuguucaca     1440

cggcccucgc gggcgcucug gaggccgaga uggauggagc caaaggccgc cuuagcucug     1500

ggcaucugaa gugccggcuc aagauggaca aacugagacu gaagggagug uccuauagcc     1560

ucuguaccgc ugccuuuaca uucaccaaaa ucccggcuga aacacugcac ggcaccguca     1620

cgguggaggu ucaauacgcc gggaccgacg gaccuugcaa ggugcccgcg cagauggccg     1680

ucgauaugca gacacuuacc ccugugggcc gccugauuac agcuaacccc gugaucaccg     1740

aaucuacgga gaacuccaaa augaugcucg aacuggaucc cccuuuuggg gacagcuaca     1800

ucguuauugg cgucggagag aagaagauca cacaccauug gcaccggucc ggcucuacca     1860

uugggaaagc cuucgaggcu acagugagag gagccaagcg cauggcggug cugggcgaca     1920

ccgccuggga uuucgggagc gucggaggcg cucuuaauuc ccucgggaaa ggcauccauc     1980

aaauuuuugg agccgcuuuc aagucucugu uuggcgggau gagcugguuc ucccagaucc     2040

ugaucggaac ccuccugaug uggcuuggcc ugaacaccaa aaaugggagc auuucucuca     2100

ugugucuggc ccugggaggc gugcuuaucu uucucuccac ggccguuagc gcugacgugg     2160

ggugcucugu cgauuucucc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6648
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6648
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacauug      720

ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca guugacaucg      780

agcuugugac caccacagug uccaauaugg ccgaagucag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ugaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcgcacgcu aagagacaga cgguggucgu gcucggcucu caggaggggg     1440

ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

ugauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc gcuguuucug     2160

ccgacguggg gugcugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6649
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6649
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uuaacgccag aaaggagaaa aagcggcgcg gcgcugacac cagcgugggg aucgucggac      120

ugcuccugac aacggccaug gcggcugaag uuaccagacg gggcuccgcc uacuauaugu      180

accuugaucg caaugacgcc ggggaggcua uuucuuuccc cacaacccug ggaaugaaca      240

aaugcuauau ccagauuaug gaucucggcc acauguguga cgccacaaug agcuacgaau      300

gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc aauaccacau      360

ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggcuaga cggucucgca      420

gagccguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu cagacauggc      480

ucgagagcag agaauacacc aagcaccuga uccgggugga gaacuggauc uuucgcaauc      540

cuggcuucgc gcuggccgcu gccgcuauug ccuggcuccu gggguccagc acgucucaga      600

aagucaucua ucuugugaug auucugcuca ucgcgcccgc cuacuccauu agaugcaucg      660

gaguuagcaa ccgggauuuu guggaaggca ugucuggggg aacauggguc gacauugugc      720

uggagcaugg cgggugugug accgucaugg cucaggauaa gccuacaguu gacaucgagc      780

uugugaccac cacagugucc aauauggccg aaguccgcag cuacugcuau gaggcuucua      840

ucuccgauau ggccagcgac uccagauguc ccacgcaagg agaagccuac cuggacaaac      900

agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg ggcaacgggu      960

guggacuguu cggcaaaggg agccugguga ccugcgcuaa guuugcgugc uccaagaaaa     1020

ugacaggaaa gagcauucag ccugagaauc ucgaauaccg caucaugcug ucuguccacg     1080

gcucccaaca cagcgggaug auugugaacg auaccggaca ugagacggac gagaaucggg     1140

ccaaaguuga aaucacaccg aacucuccca gagcugaggc cacccuuggc ggcuucggga     1200

gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu uauuaccuga     1260

caaugaauaa caagcacugg cucgugcaua aagagugguu ccacgacauu ccccugccuu     1320

ggcacgccgg ggcugauacc ggaacacccc auuggaauaa caaggaagcc cuggucgagu     1380

uuaaagacgc gcacgcuaag cggcagacgg uggucgugcu cggcucucag gagggggccg     1440

uucauaccgc ccuggcuggc gcccuugaag cugagaugga uggagccaag ggcagacuga     1500

gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag ggagugagcu     1560

auucucucug uacagcggcc uucaccuuua caaaaauccc ggcugaaacc cugcauggca     1620

cggucaccgu ggagguucag uacgccggga cagacggacc uugcaaggug cccgcucaaa     1680

uggccgucga uaugcagacc cugacaccug ugggcagacu caucaccgcg aaccccguga     1740

uuacggaauc cacagagaau agcaagauga ugcuggagcu ugaucccccu uucggggacu     1800

cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau cgcuccggca     1860

gcacaauugg gaaagccuuu gaagcuaccg ugcggggagc caagagaaug gcugugcugg     1920

gcgacaccgc cugggauuuc ggguccgucg gaggcgcccu caacucucug gggaaaggca     1980

uccaccagau cuucggagcu gcguuuaaga gccuguucgg cgggaugucc ugguuuucuc     2040

aaauucuuau cggaacacuc cugauguggc ugggccucaa uacgaagaac gggagcauuu     2100

cccugaugug ucuugcccug ggaggcgugc ucaucuuccu gagcaccgcu guuucugccg     2160

acguggggug cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  6650
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6650
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaaaaag cggcgcggcg cugacaccag cguggggauu gucggacugc      120

uccugacaac ggccauggcg gcugaaguua ccagacgggg cuccgccuac uauauguacc      180

uugaucgcaa ugacgccggg gaggcuaucu cuuuccccac aacccuggga augaacaaau      240

gcuauauuca gaucauggau cucggccaca ugugugacgc cacaaugagc uacgaaugcc      300

cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau accacaucca      360

ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcuagacgg ucucgcagag      420

ccguuacccu ucccagccau uccacacgga agcugcaaac ccgcucucag acauggcucg      480

agagcagaga auacaccaag caccugauuc ggguggagaa cuggaucuuu cgcaauccug      540

gcuucgcgcu ggccgcugcc gcuaucgccu ggcuccuggg guccagcacg ucucagaaag      600

ucauuuaucu ugugaugauc cugcucauug cgcccgccua cuccaucaga ugcauuggag      660

uuagcaaccg ggauuuugug gaaggcaugu cugggggaac augggucgac aucgugcugg      720

agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac auugagcuug      780

ugaccaccac aguguccaau auggccgaag uccgcagcua cugcuaugag gcuucuaucu      840

ccgauauggc cagcgacucc agauguccca cgcaaggaga agccuaccug gacaaacagu      900

cugauaccca guaugugugc aagcggacac ucguugacag aggcuggggc aacgggugug      960

gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc aagaaaauga     1020

caggaaagag cauccagccu gagaaucucg aauaccgcau uaugcugucu guccacggcu     1080

cccaacacag cgggaugauc gugaacgaua ccggacauga gacggacgag aaucgggcca     1140

aaguugaaau uacaccgaac ucucccagag cugaggccac ccuuggcggc uucgggagcc     1200

ugggacucga uugugaaccu cgcaccggcc uggacuuuuc cgaucuuuau uaccugacaa     1260

ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacaucccc cugccuuggc     1320

acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug gucgaguuua     1380

aagacgcgca cgcuaagcgg cagacggugg ucgugcucgg cucucaggag ggggccguuc     1440

auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc agacugagcu     1500

ccgggcaccu caaaugccgc cugaagaugg acaaacuucg gcugaaggga gugagcuauu     1560

cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug cauggcacgg     1620

ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc gcucaaaugg     1680

ccgucgauau gcagacccug acaccugugg gcagacucau caccgcgaac cccgugauca     1740

cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc ggggacucuu     1800

acauuguuau cggcgucgga gagaaaaaga uuacccacca cuggcaucgc uccggcagca     1860

caaucgggaa agccuuugaa gcuaccgugc ggggagccaa gagaauggcu gugcugggcg     1920

acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg aaaggcauuc     1980

accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg uuuucucaaa     2040

uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg agcaucuccc     2100

ugaugugucu ugcccuggga ggcgugcuca uuuuccugag caccgcuguu ucugccgacg     2160

uggggugcug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  6651
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6651
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaaaaagcgg cgcggcgcug acaccagcgu ggggaucguc ggacugcucc      120

ugacaacggc cauggcggcu gaaguuacca gacggggcuc cgccuacuau auguaccuug      180

aucgcaauga cgccggggag gcuauuucuu uccccacaac ccugggaaug aacaaaugcu      240

auauccagau uauggaucuc ggccacaugu gugacgccac aaugagcuac gaaugcccua      300

ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaauacc acauccaccu      360

gggucgugua uggaacgugu caucacaaga aaggcgaggc uagacggucu cgcagagccg      420

uuacccuucc cagccauucc acacggaagc ugcaaacccg cucucagaca uggcucgaga      480

gcagagaaua caccaagcac cugauccggg uggagaacug gauuuuucgc aauccuggcu      540

ucgcgcuggc cgcugccgcu aucgccuggc uccugggguc cagcacgucu cagaaaguca      600

ucuaucuugu gaugauucug cucaucgcgc ccgccuacuc cauuagaugc aucggaguua      660

gcaaccggga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu gugcuggagc      720

auggcgggug ugugaccguc auggcucagg auaagccuac aguugacauc gagcuuguga      780

ccaccacagu guccaauaug gccgaagucc gcagcuacug cuaugaggcu ucuauuuccg      840

auauggccag cgacuccaga ugucccacgc aaggagaagc cuaccuggac aaacagucug      900

auacccagua ugugugcaag cggacacucg uugacagagg cuggggcaac ggguguggac      960

uguucggcaa agggagccug gugaccugcg cuaaguuugc gugcuccaag aaaaugacag     1020

gaaagagcau ccagccugag aaucucgaau accgcaucau gcugucuguc cacggcuccc     1080

aacacagcgg gaugauugug aacgauaccg gacaugagac ggacgagaau cgggccaaag     1140

uugaaaucac accgaacucu cccagagcug aggccacccu uggcggcuuc gggagccugg     1200

gacucgauug ugaaccucgc accggccugg acuuuuccga ucuuuauuac cugacaauga     1260

auaacaagca cuggcucgug cauaaagagu gguuccacga cauuccccug ccuuggcacg     1320

ccggggcuga uaccggaaca ccccauugga auaacaagga agcccugguc gaguuuaaag     1380

acgcgcacgc uaagcggcag acgguggucg ugcucggcuc ucaggagggg gccguucaua     1440

ccgcccuggc uggcgcccuu gaagcugaga uggauggagc caagggcaga cugagcuccg     1500

ggcaccucaa augccgccug aagauggaca aacuucggcu gaagggagug agcuauucuc     1560

ucuguacagc ggccuucacc uuuacaaaaa ucccggcuga aacccugcau ggcacgguca     1620

ccguggaggu ucaguacgcc gggacagacg gaccuugcaa ggugcccgcu caaauggccg     1680

ucgauaugca gacccugaca ccugugggca gacucauuac cgcgaacccc gugaucacgg     1740

aauccacaga gaauagcaag augaugcugg agcuugaucc cccuuucggg gacucuuaca     1800

ucguuauugg cgucggagag aaaaagauca cccaccacug gcaucgcucc ggcagcacaa     1860

uugggaaagc cuuugaagcu accgugcggg gagccaagag aauggcugug cugggcgaca     1920

ccgccuggga uuucgggucc gucggaggcg cccucaacuc ucuggggaaa ggcauccacc     1980

agauuuucgg agcugcguuu aagagccugu ucggcgggau guccugguuu ucucaaaucc     2040

uuaucggaac acuccugaug uggcugggcc ucaauacgaa gaacgggagc auuucccuga     2100

ugugucuugc ccugggaggc gugcucaucu uccugagcac cgcuguuucu gccgacgugg     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6652
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6652
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa aaagcggcgc ggcgcugaca ccagcguggg gaucgucgga cugcuccuga      120

caacggccau ggcggcugaa guuaccagac ggggcuccgc cuacuauaug uaccuugauc      180

gcaacgacgc cggggaggcu auuucuuucc ccacaacccu gggaaugaau aaaugcuaua      240

uccagauuau ggaucucggc cacaugugug acgccacaau gagcuacgaa ugcccuaugc      300

uggaugaggg gguggaaccg gacgaugugg acuguuggug caacaccaca uccaccuggg      360

ucguguaugg aacgugucau cacaagaaag gcgaggcuag acggucucgc agagccguua      420

cccuucccag ccauuccaca cggaagcugc aaacccgcuc ucagacaugg cucgagagca      480

gagaauacac caagcaccug auccgggugg agaauuggau uuuucgcaac ccuggcuucg      540

cgcuggccgc ugccgcuauc gccuggcucc ugggguccag cacgucucag aaagucaucu      600

aucuugugau gauucugcuc aucgcgcccg ccuacuccau uagaugcauc ggaguuagca      660

aucgggauuu uguggaaggc augucugggg gaacaugggu cgacauugug cuggagcaug      720

gcgggugugu gaccgucaug gcucaggaua agccuacagu ugacaucgag cuugugacca      780

ccacaguguc caacauggcc gaaguccgca gcuacugcua ugaggcuucu auuuccgaua      840

uggccagcga cuccagaugu cccacgcaag gagaagccua ccuggacaaa cagucugaua      900

cccaguaugu gugcaagcgg acacucguug acagaggcug gggcaauggg uguggacugu      960

ucggcaaagg gagccuggug accugcgcua aguuugcgug cuccaagaaa augacaggaa     1020

agagcaucca gccugagaac cucgaauacc gcaucaugcu gucuguccac ggcucccaac     1080

acagcgggau gauugugaau gauaccggac augagacgga cgagaaccgg gccaaaguug     1140

aaaucacacc caauucuccc agagcugagg ccacccuugg cggcuucggg agccugggac     1200

ucgauuguga accucgcacc ggccuggacu uuuccgaucu uuauuaccug acaaugaaca     1260

auaagcacug gcucgugcau aaagaguggu uccacgacau uccccugccu uggcacgccg     1320

gggcugauac cggaacaccc cauuggaaca auaaggaagc ccuggucgag uuuaaagacg     1380

cgcacgcuaa gcggcagacg guggucgugc ucggcucuca ggagggggcc guucauaccg     1440

cccuggcugg cgcccuugaa gcugagaugg auggagccaa gggcagacug agcuccgggc     1500

accucaaaug ccgccugaag auggacaaac uucggcugaa gggagugagc uauucucucu     1560

guacagcggc cuucaccuuu acaaaaaucc cggcugaaac ccugcauggc acggucaccg     1620

uggagguuca guacgccggg acagacggac cuugcaaggu gcccgcucaa auggccgucg     1680

auaugcagac ccugacaccu gugggcagac ucauuaccgc gaaccccgug aucacggaau     1740

ccacagagaa cagcaagaug augcuggagc uugacccgcc uuucggggau ucuuacaucg     1800

uuauuggcgu cggagagaaa aagaucaccc accacuggca ucgcuccggc agcacaauug     1860

ggaaagccuu ugaagcuacc gugcggggag ccaagagaau ggcugugcug ggcgacaccg     1920

ccugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaaaggc auccaccaga     1980

uuuucggagc ugcguuuaag agccuguucg gcgggauguc cugguuuucu caaauccuua     2040

ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu ucccugaugu     2100

gucuugcccu gggaggcgug cucaucuucc ugagcaccgc uguuucugcc gacguggggu     2160

gcugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6653
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6653
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaaaaa gcggcgcggc gccgacacca gcguggggau cgucggacug cuccugacaa      120

cggcuauggc cgcggaaguu accagacggg gcuccgcuua cuauauguac cuugaucgca      180

acgacgccgg ggaggccauu ucuuucccca caacccuggg aaugaauaaa ugcuauaucc      240

agauuaugga ucucggccac augugugacg cuacaaugag cuacgaaugc ccuaugcugg      300

augagggggu ggaaccggac gauguggacu guuggugcaa caccacaucc accugggucg      360

uguauggaac gugucaucac aagaaaggcg aggccagacg gucucgcaga gcuguuaccc      420

uucccagcca uuccacacgg aagcugcaaa cccgcucuca gacauggcuc gagagcagag      480

aauacaccaa gcaccugauc cggguggaga auuggauuuu ucgcaacccu ggcuucgccc      540

uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaaa gucaucuauc      600

uugugaugau ucugcucauc gcccccgcgu acuccauuag augcaucgga guuagcaauc      660

gggauuuugu ggaaggcaug ucugggggaa caugggucga cauugugcug gagcauggcg      720

ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu gugaccacca      780

caguguccaa cauggcugaa guccgcagcu acugcuauga ggccucuauu uccgauaugg      840

cuagcgacuc cagauguccc acgcaaggag aagccuaccu ggacaaacag ucugauaccc      900

aguaugugug caagcggaca cucguugaca gaggcugggg caaugggugu ggacuguucg      960

gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caagaaaaug acaggaaaga     1020

gcauccagcc ugagaaccuc gaauaccgca ucaugcuguc uguccacggc ucccaacaca     1080

gcgggaugau ugugaaugau accggacaug agacggacga gaaccgggcg aaaguugaaa     1140

ucacacccaa uucucccaga gccgaggcua cccuuggcgg cuucgggagc cugggacucg     1200

auugugaacc ucgcaccggc cuggacuuuu ccgaucuuua uuaccugaca augaacaaua     1260

agcacuggcu cgugcauaaa gagugguucc acgacauucc ccugccuugg cacgccgggg     1320

ccgauaccgg aacaccccau uggaacaaua aggaagcucu ggucgaguuu aaagacgccc     1380

acgcgaagcg gcagacggug gucgugcucg gcucucagga gggggcuguu cauaccgccc     1440

uggccggcgc ucuugaagcc gagauggaug gagcuaaggg cagacugagc uccgggcacc     1500

ucaaaugccg ccugaagaug gacaaacuuc ggcugaaggg agugagcuau ucucucugua     1560

cagccgcguu caccuuuaca aaaaucccgg ccgaaacccu gcauggcacg gucaccgugg     1620

agguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug gcugucgaua     1680

ugcagacccu gacaccugug ggcagacuca uuaccgccaa ccccgugauc acggaaucca     1740

cagagaacag caagaugaug cuggagcuug acccgccuuu cggggauucu uacaucguua     1800

uuggcgucgg agagaaaaag aucacccacc acuggcaucg cuccggcagc acaauuggga     1860

aagcguuuga agccaccgug cggggagcua agagaauggc cgugcugggc gacaccgcuu     1920

gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc caccagauuu     1980

ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa auccuuaucg     2040

gaacacuccu gauguggcug ggccucaaca cgaagaaugg gagcauuucc cugauguguc     2100

uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac guggggugcu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6654
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6654
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaaaaagag acggggcgcc gacaccagcg uggggaucgu cggacugcuc cugacaacgg      120

cuauggccgc ggaaguuacc cgcagaggcu ccgcuuacua uauguaccuu gaucggaacg      180

acgccgggga ggccauuucu uuccccacaa cccugggaau gaauaaaugc uauauccaga      240

uuauggaucu cggccacaug ugugacgcua caaugagcua cgaaugcccu augcuggaug      300

agggggugga accggacgau guggacuguu ggugcaacac cacauccacc ugggucgugu      360

auggaacgug ucaucacaag aaaggcgagg cccgcagauc ucggcgcgcu guuacccuuc      420

ccagccauuc cacaagaaag cugcaaaccc ggucucagac auggcucgag agccgcgaau      480

acaccaagca ccugaucaga guggagaauu ggauuuuucg gaacccuggc uucgcccugg      540

cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc aucuaucuug      600

ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu agcaauagag      660

auuuugugga aggcaugucu gggggaacau gggucgacau ugugcuggag cauggcgggu      720

gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug accaccacag      780

uguccaacau ggcugaaguc cggagcuacu gcuaugaggc cucuauuucc gauauggcua      840

gcgacucccg cugucccacg caaggagaag ccuaccugga caaacagucu gauacccagu      900

augugugcaa gagaacacuc guugaccggg gcuggggcaa ugggugugga cuguucggca      960

aagggagccu ggugaccugc gccaaguuug cuugcuccaa gaaaaugaca ggaaagagca     1020

uccagccuga gaaccucgaa uacagaauca ugcugucugu ccacggcucc caacacagcg     1080

ggaugauugu gaaugauacc ggacaugaga cggacgagaa ccgcgcgaaa guugaaauca     1140

cacccaauuc uccccgggcc gaggcuaccc uuggcggcuu cgggagccug ggacucgauu     1200

gugaaccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug aacaauaagc     1260

acuggcucgu gcauaaagag ugguuccacg acauuccccu gccuuggcac gccggggccg     1320

auaccggaac accccauugg aacaauaagg aagcucuggu cgaguuuaaa gacgcccacg     1380

cgaagcgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau accgcccugg     1440

ccggcgcucu ugaagccgag auggauggag cuaagggccg gcugagcucc gggcaccuca     1500

aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu cucuguacag     1560

ccgcguucac cuuuacaaaa aucccggccg aaacccugca uggcacgguc accguggagg     1620

uucaguacgc ugggacagac ggaccuugca aggugcccgc ccaaauggcu gucgauaugc     1680

agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg gaauccacag     1740

agaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac aucguuauug     1800

gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca auugggaaag     1860

cguuugaagc caccgugcgc ggagcuaagc ggauggccgu gcugggcgac accgcuuggg     1920

auuucggguc cgucggaggc gcccucaauu cucuggggaa aggcauccac cagauuuucg     1980

gagccgcuuu uaagagccug uucggcggga uguccugguu uucucaaauc cuuaucggaa     2040

cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug augugucuug     2100

cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacgug gggugcugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6655
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6655
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagacg gggcgccgac accagcgugg ggaucgucgg acugcuccug acaacggcua      120

uggccgcgga aguuacccgc agaggcuccg cuuacuauau guaccuugau cggaacgacg      180

ccggggaggc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau auccagauua      240

uggaucucgg ccacaugugu gacgcuacaa ugagcuacga augcccuaug cuggaugagg      300

ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg gucguguaug      360

gaacguguca ucacaaaaag ggcgaggccc gcagaucucg gcgcgcuguu acccuuccca      420

gccauuccac aagaaaacug caaacccggu cucagacaug gcucgagagc cgcgaauaca      480

ccaagcaccu gaucagagug gagaauugga uuuuucggaa cccuggcuuc gcccuggcgg      540

ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaggucauc uaucuuguga      600

ugauucugcu caucgccccc gcguacucca uucgcugcau cggaguuagc aauagagauu      660

uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau ggcgggugug      720

ugaccgucau ggcccaggau aaaccuacag uugacaucga gcuugugacc accacagugu      780

ccaacauggc ugaaguccgg agcuacugcu augaggccuc uauuuccgau auggcuagcg      840

acucccgcug ucccacgcaa ggagaagccu accuggacaa gcagucugau acccaguaug      900

ugugcaaaag aacacucguu gaccggggcu ggggcaaugg guguggacug uucggcaagg      960

ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa gaugacagga aaaagcaucc     1020

agccugagaa ccucgaauac agaaucaugc ugucugucca cggcucccaa cacagcggga     1080

ugauugugaa ugauaccgga caugagacgg acgagaaccg cgcgaagguu gaaaucacac     1140

ccaauucucc ccgggccgag gcuacccuug gcggcuucgg gagccuggga cucgauugug     1200

aaccuagaac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac aauaaacacu     1260

ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc ggggccgaua     1320

ccggaacacc ccauuggaac aauaaagaag cucuggucga guuuaaggac gcccacgcga     1380

aacgccagac gguggucgug cucggcucuc aggagggggc uguucauacc gcccuggccg     1440

gcgcucuuga agccgagaug gauggagcua agggccggcu gagcuccggg caccucaagu     1500

gcagacugaa aauggacaaa cuucgccuga agggagugag cuauucucuc uguacagccg     1560

cguucaccuu uacaaagauc ccggccgaaa cccugcaugg cacggucacc guggagguuc     1620

aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc gauaugcaga     1680

cccugacacc ugugggccgg cucauuaccg ccaaccccgu gaucacggaa uccacagaga     1740

acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc guuauuggcg     1800

ucggagaaaa gaaaaucacc caccacuggc auagauccgg cagcacaauu gggaaggcgu     1860

uugaggccac cgugcgcgga gcuaaacgga uggccgugcu gggcgacacc gcuugggauu     1920

ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag auuuucggag     1980

ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu aucggaacac     2040

uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug ugucuugcgc     2100

ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacgugggg ugcugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6656
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6656
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagacgggg cgccgacacc agcgugggga ucgucggacu gcuccugaca acggcuaugg      120

ccgcggaggu uacccgcaga ggcuccgcuu acuauaugua ccuugaucgg aacgacgccg      180

gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc cagauuaugg      240

aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug gaugaagggg      300

uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc guguauggaa      360

cgugucauca caaaaagggc gaagcccgca gaucucggcg cgcuguuacc cuucccagcc      420

auuccacaag aaaacugcaa acccggucuc agacauggcu cgagagccgc gaguacacca      480

agcaccugau cagaguggaa aauuggauuu uucggaaccc uggcuucgcc cuggcggccg      540

cugccaucgc uuggcuccug ggguccagca cgucucagaa ggucaucuau cuugugauga      600

uucugcucau cgcccccgcg uacuccauuc gcugcaucgg aguuagcaau agagauuuug      660

uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc ggguguguga      720

ccgucauggc ccaggauaaa ccuacaguug acaucgagcu ugugaccacc acagugucca      780

acauggcuga gguccggagc uacugcuaug aagccucuau uuccgauaug gcuagcgacu      840

cccgcugucc cacgcaagga gaggccuacc uggacaagca gucugauacc caguaugugu      900

gcaaaagaac acucguugac cggggcuggg gcaaugggug uggacuguuc ggcaagggga      960

gccuggugac cugcgccaaa uuugcuugcu ccaagaagau gacaggaaaa agcauccagc     1020

cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac agcgggauga     1080

uugugaauga uaccggacau gaaacggacg agaaccgcgc gaagguugag aucacaccca     1140

auucuccccg ggccgaagcu acccuuggcg gcuucgggag ccugggacuc gauugugagc     1200

cuagaaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau aaacacuggc     1260

ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg gccgauaccg     1320

gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc cacgcgaaac     1380

gccagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc cuggccggcg     1440

cucuugaggc cgaaauggau ggagcuaagg gccggcugag cuccgggcac cucaagugca     1500

gacugaaaau ggacaaacuu cgccugaagg gagugagcua uucucucugu acagccgcgu     1560

ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug gaaguucagu     1620

acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau augcagaccc     1680

ugacaccugu gggccggcuc auuaccgcca accccgugau cacggagucc acagaaaaca     1740

gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu auuggcgucg     1800

gagagaagaa aaucacccac cacuggcaua gauccggcag cacaauuggg aaggcguuug     1860

aagccaccgu gcgcggagcu aaacggaugg ccgugcuggg cgacaccgcu ugggauuucg     1920

gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu uucggagccg     1980

cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc ggaacacucc     2040

ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu cuugcgcugg     2100

gaggcgugcu caucuuccug agcaccgccg uuucugcuga cguggggugc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6657
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6657
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gacggggcgc cgacaccagc guggggaucg ucggacugcu ccugacaacg gcuauggccg      120

cggagguuac ccgcagaggc uccgcuuacu auauguaccu ugaucggaac gacgccgggg      180

aagccauuuc uuuccccaca acccugggaa ugaauaaaug cuauauccag auuauggauc      240

ucggccacau gugugacgcu acaaugagcu acgagugccc uaugcuggau gaaggggugg      300

agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug uauggaacgu      360

gucaucacaa gaaaggcgaa gcccgcagau cucggcgcgc uguuacccuu cccagccauu      420

ccacaagaaa gcugcaaacc cggucucaga cauggcucga gagccgcgag uacaccaaac      480

accugaucag aguggaaaau uggauuuuuc ggaacccugg cuucgcccug gcggccgcug      540

ccaucgcuug gcuccugggg uccagcacgu cucagaaggu caucuaucuu gugaugauuc      600

ugcucaucgc ccccgcguac uccauucgcu gcaucggagu uagcaauaga gauuuugugg      660

agggcauguc ugggggaaca ugggucgaca uugugcugga acauggcggg ugugugaccg      720

ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca guguccaaca      780

uggcugaggu ccggagcuac ugcuaugaag ccucuauuuc cgauauggcu agcgacuccc      840

gcugucccac gcaaggagag gccuaccugg acaaacaguc ugauacccag uaugugugca      900

agagaacacu cguugaccgg ggcuggggca augggugugg acuguucggc aaagggagcc      960

uggugaccug cgccaaguuu gcuugcucca aaaagaugac aggaaagagc auccagccug     1020

aaaaccucga guacagaauc augcugucug uccacggcuc ccaacacagc gggaugauug     1080

ugaaugauac cggacaugaa acggacgaga accgcgcgaa aguugagauc acacccaauu     1140

cuccccgggc cgaagcuacc cuuggcggcu ucgggagccu gggacucgau ugugagccua     1200

gaaccggccu ggacuuuucc gaucuuuauu accugacaau gaacaauaag cacuggcucg     1260

ugcauaaaga augguuccac gacauucccc ugccuuggca cgccggggcc gauaccggaa     1320

caccccauug gaacaauaag gaggcucugg ucgaauuuaa agacgcccac gcgaagcgcc     1380

agacgguggu cgugcucggc ucucaggagg gggcuguuca uaccgcccug gccggcgcuc     1440

uugaggccga aauggaugga gcuaaaggcc ggcugagcuc cgggcaccuc aagugcagac     1500

ugaagaugga caaacuucgc cugaagggag ugagcuauuc ucucuguaca gccgcguuca     1560

ccuuuacaaa aaucccggcc gagacccugc auggcacggu caccguggaa guucaguacg     1620

cugggacaga cggaccuugc aaggugcccg cccaaauggc ugucgauaug cagacccuga     1680

caccuguggg ccggcucauu accgccaacc ccgugaucac ggaguccaca gaaaacagca     1740

aaaugaugcu ggagcuugac ccgccuuucg gggauucuua caucguuauu ggcgucggag     1800

agaagaagau cacccaccac uggcauagau ccggcagcac aauugggaaa gcguuugaag     1860

ccaccgugcg cggagcuaag cggauggccg ugcugggcga caccgcuugg gauuucgggu     1920

ccgucggagg cgcccucaau ucucugggga aaggcaucca ccagauuuuc ggagccgcuu     1980

uuaagagccu guucggcggg auguccuggu uuucucaaau ccuuaucgga acacuccuga     2040

uguggcuggg ccucaacacg aaaaauggga gcauuucccu gaugugucuu gcgcugggag     2100

gcgugcucau cuuccugagc accgccguuu cugcugacgu ggggugcuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6658
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6658
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgccga caccagcgug gggaucgucg gacugcuccu gacaacggcu auggccgcgg      120

agguuacccg cagaggcucc gcuuacuaua uguaccuuga ucggaacgac gccggggaag      180

ccauuucuuu ccccacaacc cugggaauga auaagugcua uauccagauu auggaucucg      240

gccacaugug ugacgcuaca augagcuacg agugcccuau gcuggaugaa gggguggagc      300

cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau ggaacguguc      360

aucacaaaaa gggcgaagcc cgcagaucuc ggcgcgcugu uacccuuccc agccauucca      420

caagaaaacu gcaaacccgg ucucagacau ggcucgagag ccgcgaguac accaagcacc      480

ugaucagagu ggaaaauugg auuuuucgga acccuggcuu cgcccuggcg gccgcugcca      540

ucgcuuggcu ccuggggucc agcacgucuc agaaagucau cuaucuugug augauucugc      600

ucaucgcccc cgcguacucc auucgcugca ucggaguuag caauagagau uuuguggagg      660

gcaugucugg gggaacaugg gucgacauug ugcuggaaca uggcgggugu gugaccguca      720

uggcccagga uaagccuaca guugacaucg agcuugugac caccacagug uccaacaugg      780

cugagguccg gagcuacugc uaugaagccu cuauuuccga uauggcuagc gacucccgcu      840

gucccacgca aggagaggcc uaccuggaca agcagucuga uacccaguau gugugcaaaa      900

gaacacucgu ugaccggggc uggggcaaug gguguggacu guucggcaag gggagccugg      960

ugaccugcgc caaauuugcu ugcuccaaga aaaugacagg aaagagcauc cagccugaaa     1020

accucgagua cagaaucaug cugucugucc acggcuccca acacagcggg augauuguga     1080

augauaccgg acaugaaacg gacgagaacc gcgcgaaggu ugagaucaca cccaauucuc     1140

cccgggccga agcuacccuu ggcggcuucg ggagccuggg acucgauugu gagccuagaa     1200

ccggccugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac uggcucgugc     1260

auaaggaaug guuccacgac auuccccugc cuuggcacgc cggggccgau accggaacac     1320

cccauuggaa caauaaagag gcucuggucg aauuuaagga cgcccacgcg aaacgccaga     1380

cgguggucgu gcucggcucu caggaggggg cuguucauac cgcccuggcc ggcgcucuug     1440

aggccgaaau ggauggagcu aagggccggc ugagcuccgg gcaccucaaa ugcagacuga     1500

agauggacaa acuucgccug aagggaguga gcuauucucu cuguacagcc gcguucaccu     1560

uuacaaagau cccggccgag acccugcaug gcacggucac cguggaaguu caguacgcug     1620

ggacagacgg accuugcaaa gugcccgccc aaauggcugu cgauaugcag acccugacac     1680

cugugggccg gcucauuacc gccaaccccg ugaucacgga guccacagaa aacagcaaga     1740

ugaugcugga gcuugacccg ccuuucgggg auucuuacau cguuauuggc gucggagaga     1800

aaaagaucac ccaccacugg cauagauccg gcagcacaau ugggaaggcg uuugaagcca     1860

ccgugcgcgg agcuaaacgg auggccgugc ugggcgacac cgcuugggau uucggguccg     1920

ucggaggcgc ccucaauucu cuggggaagg gcauccacca gauuuucgga gccgcuuuua     1980

aaagccuguu cggcgggaug uccugguuuu cucaaauccu uaucggaaca cuccugaugu     2040

ggcugggccu caacacgaag aaugggagca uuucccugau gugucuugcg cugggaggcg     2100

ugcucaucuu ccugagcacc gccguuucug cugacguggg gugcugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6659
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6659
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug gccgcggagg      120

uuacccggcg cggcuccgcu uacuauaugu accuugauag aaacgacgcc ggggaagcca      180

uuucuuuccc cacaacccug ggaaugaaua agugcuauau ccagauuaug gaucucggcc      240

acauguguga cgcuacaaug agcuacgagu gcccuaugcu ggaugaaggg guggagccgg      300

acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga acgugucauc      360

acaaaaaggg cgaagcccgg cgcucuagac gggcuguuac ccuucccagc cauuccacac      420

gcaaacugca aaccagaucu cagacauggc ucgagagccg ggaguacacc aagcaccuga      480

uccgcgugga aaauuggauu uuuagaaacc cuggcuucgc ccuggcggcc gcugccaucg      540

cuuggcuccu gggguccagc acgucucaga aagucaucua ucuugugaug auucugcuca      600

ucgcccccgc guacuccauu cggugcaucg gaguuagcaa ucgcgauuuu guggagggca      660

ugucuggggg aacauggguc gacauugugc uggaacaugg cgggugugug accgucaugg      720

cccaggauaa gccuacaguu gacaucgagc uugugaccac cacagugucc aacauggcug      780

aggucagaag cuacugcuau gaagccucua uuuccgauau ggcuagcgac ucccgguguc      840

ccacgcaagg agaggccuac cuggacaagc agucugauac ccaguaugug ugcaaacgca      900

cacucguuga cagaggcugg ggcaaugggu guggacuguu cggcaagggg agccugguga      960

ccugcgccaa auuugcuugc uccaagaaaa ugacaggaaa gagcauccag ccugaaaacc     1020

ucgaguaccg gaucaugcug ucuguccacg gcucccaaca cagcgggaug auugugaaug     1080

auaccggaca ugaaacggac gagaacagag cgaagguuga gaucacaccc aauucucccc     1140

gcgccgaagc uacccuuggc ggcuucggga gccugggacu cgauugugag ccucggaccg     1200

gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaaacacugg cucgugcaua     1260

aggaaugguu ccacgacauu ccccugccuu ggcacgccgg ggccgauacc ggaacacccc     1320

auuggaacaa uaaagaggcu cuggucgaau uuaaggacgc ccacgcgaaa agacagacgg     1380

uggucgugcu cggcucucag gagggggcug uucauaccgc ccuggccggc gcucuugagg     1440

ccgaaaugga uggagcuaag ggccgccuga gcuccgggca ccucaaaugc cggcugaaga     1500

uggacaaacu uagacugaag ggagugagcu auucucucug uacagccgcg uucaccuuua     1560

caaagauccc ggccgagacc cugcauggca cggucaccgu ggaaguucag uacgcuggga     1620

cagacggacc uugcaaagug cccgcccaaa uggcugucga uaugcagacc cugacaccug     1680

ugggccgccu cauuaccgcc aaccccguga ucacggaguc cacagaaaac agcaagauga     1740

ugcuggagcu ugacccgccu uucggggauu cuuacaucgu uauuggcguc ggagagaaaa     1800

agaucaccca ccacuggcau cgguccggca gcacaauugg gaaggcguuu gaagccaccg     1860

ugagaggagc uaaacgcaug gccgugcugg gcgacaccgc uugggauuuc ggguccgucg     1920

gaggcgcccu caauucucug gggaagggca uccaccagau uuucggagcc gcuuuuaaaa     1980

gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc cugauguggc     2040

ugggccucaa cacgaagaau gggagcauuu cccugaugug ucuugcgcug ggaggcgugc     2100

ucaucuuccu gagcaccgcc guuucugcug acguggggug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6660
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6660
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gucggacugc uccugacaac ggcuauggcc gcggagguua      120

ccagacgggg cuccgcuuac uauauguacc uugaucgcaa cgacgccggg gaagccauuu      180

cuuuccccac aacccuggga augaauaagu gcuauaucca gauuauggau cucggccaca      240

ugugugacgc uacaaugagc uacgagugcc cuaugcugga ugaaggggug gagccggacg      300

auguggacug uuggugcaac accacaucca ccugggucgu guauggaacg ugucaucaca      360

aaaagggcga agccagacgg ucucgcagag cuguuacccu ucccagccau uccacacgga      420

aacugcaaac ccgcucucag acauggcucg agagcagaga guacaccaag caccugaucc      480

ggguggaaaa uuggauuuuu cgcaacccug gcuucgcccu ggcggccgcu gccaucgcuu      540

ggcuccuggg guccagcacg ucucagaaag ucaucuaucu ugugaugauu cugcucaucg      600

cccccgcgua cuccauuaga ugcaucggag uuagcaaucg ggauuuugug gagggcaugu      660

cugggggaac augggucgac auugugcugg aacauggcgg gugugugacc gucauggccc      720

aggauaagcc uacaguugac aucgagcuug ugaccaccac aguguccaac auggcugagg      780

uccgcagcua cugcuaugaa gccucuauuu ccgauauggc uagcgacucc agauguccca      840

cgcaaggaga ggccuaccug gacaagcagu cugauaccca guaugugugc aaacggacac      900

ucguugaccg cggcuggggc aaugggugug gacuguucgg caaggggagc cuggugaccu      960

gcgccaaauu ugcuugcucc aagaaaauga caggaaagag cauccagccu gaaaaccucg     1020

aguacagaau caugcugucu guccacggcu cccaacacag cgggaugauu gugaaugaua     1080

ccggacauga aacggacgag aaccgggcga agguugagau cacacccaau ucucccagag     1140

ccgaagcuac ccuuggcggc uucgggagcc ugggacucga uugugagccu cgcaccggcc     1200

uggacuuuuc cgaucuuuau uaccugacaa ugaacaauaa acacuggcuc gugcauaagg     1260

aaugguucca cgacauuccc cugccuuggc acgccggggc cgauaccgga acaccccauu     1320

ggaacaauaa agaggcucug gucgaauuua aggacgccca cgcgaaacgg cagacggugg     1380

ucgugcucgg cucucaggag ggggcuguuc auaccgcccu ggccggcgcu cuugaggccg     1440

aaauggaugg agcuaagggc agacugagcu ccgggcaccu caaaugccgc cugaagaugg     1500

acaaacuucg gcugaaggga gugagcuauu cucucuguac agccgcguuc accuuuacaa     1560

agaucccggc cgagacccug cauggcacgg ucaccgugga aguucaguac gcugggacag     1620

acggaccuug caaagugccc gcccaaaugg cugucgauau gcagacccug acaccugugg     1680

gcagacucau uaccgccaac cccgugauca cggaguccac agaaaacagc aagaugaugc     1740

uggagcuuga cccgccuuuc ggggauucuu acaucguuau uggcgucgga gagaaaaaga     1800

ucacccacca cuggcaucgc uccggcagca caauugggaa ggcguuugaa gccaccgugc     1860

ggggagcuaa aagaauggcc gugcugggcg acaccgcuug ggauuucggg uccgucggag     1920

gcgcccucaa uucucugggg aagggcaucc accagauuuu cggagccgcu uuuaaaagcc     1980

uguucggcgg gauguccugg uuuucucaaa uccuuaucgg aacacuccug auguggcugg     2040

gccucaacac gaagaauggg agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca     2100

ucuuccugag caccgccguu ucugcugacg uggggugcug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6661
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6661
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucguc gggcugcucc ugacaacggc uauggccgcg gagguuacca      120

gacggggauc cgcuuacuau auguaccuug aucgcaacga cgccggcgaa gccauuucuu      180

uccccacaac ccuggggaug aauaagugcu auauccagau uauggaucuc ggacacaugu      240

gugacgcuac aaugagcuac gagugcccua ugcuggauga aggcguggag ccggacgaug      300

uggacuguug gugcaacacc acauccaccu gggucgugua ugggacgugu caucacaaaa      360

agggagaagc cagacggucu cgcagagcug uuacccuucc cagccauucc acacggaaac      420

ugcaaacccg cucucagaca uggcucgaga gcagagagua caccaagcac cugauccggg      480

uggaaaauug gauuuuucgc aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc      540

uccugggcuc cagcacgucu cagaaaguca ucuaucuugu gaugauucug cucaucgccc      600

ccgcguacuc cauuagaugc aucgggguua gcaaucggga uuuuguggag ggaaugucug      660

gcgggacaug ggucgacauu gugcuggaac auggaggcug ugugaccguc auggcccagg      720

auaagccuac aguugacauc gagcuuguga ccaccacagu guccaacaug gcugaggucc      780

gcagcuacug cuaugaagcc ucuauuuccg auauggcuag cgacuccaga ugucccacgc      840

aaggggaggc cuaccuggac aagcagucug auacccagua ugugugcaaa cggacacucg      900

uugaccgcgg auggggcaau ggcugugggc uguucggaaa gggcagccug gugaccugcg      960

ccaaauuugc uugcuccaag aaaaugacag gaaagagcau ccagccugaa aaccucgagu     1020

acagaaucau gcugucuguc cacggguccc aacacagcgg caugauugug aaugauaccg     1080

ggcaugaaac ggacgagaac cgggcgaagg uugagaucac acccaauucu cccagagccg     1140

aagcuacccu uggaggcuuc ggcagccugg ggcucgauug ugagccucgc accggacugg     1200

acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug cauaaggaau     1260

gguuccacga cauuccccug ccuuggcacg ccggcgccga uaccgggaca ccccauugga     1320

acaauaaaga ggcucugguc gaauuuaagg acgcccacgc gaaacggcag acgguggucg     1380

ugcucggauc ucaggagggc gcuguucaua ccgcccuggc cggggcucuu gaggccgaaa     1440

uggauggcgc uaagggaaga cugagcuccg gccaccucaa augccgccug aagauggaca     1500

agcugcggcu gaaaggggug agcuauucuc ucuguacagc cgcguucacc uuuacaaaga     1560

ucccggccga gacccugcau ggaacgguca ccguggaagu ucaguacgcu ggcacagacg     1620

ggccuugcaa agugcccgcc caaauggcug ucgauaugca gacccugaca ccugugggaa     1680

gacucauuac cgccaacccc gugaucacgg aguccacaga aaacagcaag augaugcugg     1740

agcuugaccc gccuuucggc gauucuuaca ucguuauugg ggucggcgag aaaaagauca     1800

cccaccacug gcaucgcucc ggaagcacaa uuggcaaggc guuugaagcc accgugagag     1860

gggcuaaacg gauggccgug cugggagaca ccgcuuggga uuucggcucc gucgggggag     1920

cccucaauuc ucugggcaag gggauccacc agauuuucgg cgccgcuuuu aaaagccugu     1980

ucggaggcau guccugguuu ucucaaaucc uuaucgggac acuccugaug uggcugggac     2040

ucaacacgaa gaauggcagc auuucccuga ugugucuugc gcuuggggga gugcucaucu     2100

uccugagcac cgccguuucu gcugacgugg gcugcugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6662
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6662
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgucggg cugcuccuga caacggccau ggcugccgag guuaccagac      120

ggggauccgc guacuauaug uaccuugauc gcaacgacgc uggcgaagcc auuucuuucc      180

ccacaacccu ggggaugaau aagugcuaua uccagauuau ggaucucgga cacaugugug      240

acgccacaau gagcuacgag ugcccuaugc uggaugaagg cguggagccg gacgaugugg      300

acuguuggug caacaccaca uccaccuggg ucguguaugg gacgugucau cacaaaaagg      360

gagaagcuag acggucucgc agagccguua cccuucccag ccauuccaca cggaaacugc      420

aaacccgcuc ucagacaugg cucgagagca gagaguacac caagcaccug auccgggugg      480

aaaauuggau uuuucgcaac ccuggcuucg cucuggccgc ggccgcuauc gccuggcucc      540

ugggcuccag cacgucucag aaagucaucu aucuugugau gauucugcuc aucgcucccg      600

ccuacuccau uagaugcauc gggguuagca aucgggauuu uguggaggga augucuggcg      660

ggacaugggu cgacauugug cuggaacaug gaggcugugu gaccgucaug gcgcaggaua      720

agccuacagu ugacaucgag cuugugacca ccacaguguc caacauggcc gagguccgca      780

gcuacugcua ugaagcuagc auuuccgaua uggccagcga cuccagaugu cccacgcaag      840

gggaggcuua ccuggacaag cagucugaua cccaguaugu gugcaaacgg acacucguug      900

accgcggaug gggcaauggc ugugggcugu ucggaaaggg cagccuggug accugcgcca      960

aauuugccug cuccaagaaa augacaggga agucuaucca gccugaaaac cucgaguaca     1020

gaaucaugcu gucuguccac ggaucccaac acagcggcau gauugugaau gauaccgggc     1080

augaaacgga cgagaaccgg gcuaagguug agaucacacc caauucuccc agagcggaag     1140

ccacccuugg aggcuucggc agccuggggc ucgauuguga gccucgcacc ggacuggacu     1200

uuuccgaucu uuauuaccug acaaugaaca auaaacacug gcucgugcau aaggaauggu     1260

uccacgacau uccccugccu uggcacgcug gcgccgauac cgggacaccc cauuggaaca     1320

auaaagaggc ccuggucgaa uuuaaggacg cucacgccaa acggcagacg guggucgugc     1380

ucggaucuca ggagggcgcg guucauaccg cucuggccgg ggcccuugag gcugaaaugg     1440

auggcgccaa gggaagacug agcuccggcc accucaaaug ccgccugaag auggacaagc     1500

ugcggcugaa aggggugagc uauucucucu guacagcugc cuucaccuuu acaaagaucc     1560

cggcggagac ccugcaugga acggucaccg uggaaguuca guacgccggc acagacgggc     1620

cuugcaaagu gcccgcucaa auggccgucg auaugcagac ccugacaccu gugggaagac     1680

ucauuaccgc uaaccccgug aucacggagu ccacagaaaa cagcaagaug augcuggagc     1740

uugacccgcc uuucggcgau ucuuacaucg uuauuggggu cggcgagaaa aagaucaccc     1800

accacuggca ucgcuccgga agcacaauug gcaaggccuu ugaagcgacc gugagagggg     1860

ccaaacggau ggcugugcug ggagacaccg ccugggauuu cggcuccguc gggggagcuc     1920

ucaauucucu gggcaagggg auccaccaga uuuucggcgc cgccuuuaaa agccuguucg     1980

gaggcauguc cugguuuucu caaauccuua ucgggacacu ccugaugugg cugggacuca     2040

acacgaagaa uggcagcauu ucccugaugu gucuugcucu ugggggagug cucaucuucc     2100

ugagcaccgc gguuucugcc gacgugggcu gcugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6663
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6663
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccgau gugggcugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6664
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6664
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgcgguu accagacggg      120

gauccgcuua cuauauguac cuugaccgca acgaugccgg cgaggccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

cuacaaugag cuacgaaugc ccuaugcugg acgagggcgu ggaaccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aggccagacg gucucgcaga gcuguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gaaagcagag aguacaccaa gcaccugauc cggguggaga      480

auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcccccgcgu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggaaggaaug ucuggcggga      660

caugggucga uauugugcug gagcauggag gcugugugac cgucauggcc caggacaagc      720

cuacaguuga uaucgaacuu gugaccacca caguguccaa cauggcugag guccgcagcu      780

acugcuauga ggccucuauu uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aagccuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugcuugcuc caagaaaaug acagggaagu cuauccagcc ugagaaccuc gaauacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

agacggauga aaaccgggcg aagguugaga ucacaccgaa uagccccaga gccgaggcua     1140

cccuuggagg cuucggcagc cuggggcucg acugugaacc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gagugguucc     1260

acgauauucc ccugccuugg cacgccggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaagcucu ggucgaguuu aaggaugccc acgcgaaacg gcagacggug gucgugcucg     1380

gaucucagga aggcgcuguu cauaccgccc uggccggggc ucuugaggcc gagauggacg     1440

gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca aagaucccgg     1560

ccgaaacccu gcauggaacg gucaccgugg agguucagua cgcuggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcugucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgccaa ccccgugauc acggaaucca cagagaacag caagaugaug cuggaacuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggcguuuga ggccaccgug agaggggcua     1860

aacggauggc cgugcuggga gauaccgcuu gggacuucgg cuccgucggg ggagcccuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc aucuuccuga     2100

gcaccgccgu uucugcugau gugggcugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6665
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6665
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug gccgcggcug      120

uuacccggcg cggcuccgcc uacuauaugu accuugauag aaacgacgcc ggggaggcua      180

uuucuuuccc cacaacccug ggaaugaaua agugcuauau ccagauuaug gaucucggcc      240

acauguguga cgccacaaug agcuacgaau gcccuaugcu ggaugagggg guggaaccgg      300

acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga acgugucauc      360

acaaaaaggg cgaggcucgg cgcucuagac gggccguuac ccuucccagc cauuccacac      420

gcaaacugca aaccagaucu cagacauggc ucgaaagccg ggaguacacc aagcaccuga      480

uccgcgugga gaauuggauu uuuagaaacc cuggcuucgc gcuggccgcu gccgcuaucg      540

ccuggcuccu gggguccagc acgucucaga aagucaucua ucuugugaug auucugcuca      600

ucgcgcccgc cuacuccauu cggugcaucg gaguuagcaa ucgcgauuuu guggaaggca      660

ugucuggggg aacauggguc gacauugugc uggagcaugg cgggugugug accgucaugg      720

cucaggauaa gccuacaguu gacaucgaac uugugaccac cacagugucc aacauggccg      780

aggucagaag cuacugcuau gaggcuucua uuuccgauau ggccagcgac ucccgguguc      840

ccacgcaagg agaagccuac cuggacaagc agucugauac ccaguaugug ugcaaacgca      900

cacucguuga cagaggcugg ggcaaugggu guggacuguu cggcaagggg agccugguga      960

ccugcgcuaa auuugcgugc uccaagaaaa ugacaggaaa gagcauccag ccugagaacc     1020

ucgaauaccg gaucaugcug ucuguccacg gcucccaaca cagcgggaug auugugaaug     1080

auaccggaca ugagacggac gaaaacagag ccaagguuga gaucacaccc aauucucccc     1140

gcgcugaggc cacccuuggc ggcuucggga gccugggacu cgauugugaa ccucggaccg     1200

gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaaacacugg cucgugcaua     1260

aggagugguu ccacgacauu ccccugccuu ggcacgccgg ggcugauacc ggaacacccc     1320

auuggaacaa uaaagaagcc cuggucgagu uuaaggacgc gcacgcuaaa agacagacgg     1380

uggucgugcu cggcucucag gaaggggccg uucauaccgc ccuggcuggc gcccuugagg     1440

cugagaugga uggagccaag ggccgccuga gcuccgggca ccucaaaugc cggcugaaga     1500

uggacaaacu uagacugaag ggagugagcu auucucucug uacagcggcc uucaccuuua     1560

caaagauccc ggcugaaacc cugcauggca cggucaccgu ggagguucag uacgccggga     1620

cagacggacc uugcaaagug cccgcucaaa uggccgucga uaugcagacc cugacaccug     1680

ugggccgccu cauuaccgcg aaccccguga ucacggaauc cacagagaac agcaagauga     1740

ugcuggaacu ugacccgccu uucggggauu cuuacaucgu uauuggcguc ggagagaaaa     1800

agaucaccca ccacuggcau cgguccggca gcacaauugg gaaggccuuu gaggcuaccg     1860

ugagaggagc caaacgcaug gcugugcugg gcgacaccgc cugggauuuc ggguccgucg     1920

gaggcgcccu caauucucug gggaagggca uccaccagau uuucggagcu gcguuuaaaa     1980

gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc cugauguggc     2040

ugggccucaa cacgaagaau gggagcauuu cccugaugug ucuugcccug ggaggcgugc     2100

ucaucuuccu gagcaccgcu guuucugccg acguggggug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6666
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6666
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc ccggaagcgc agaucucacg acgugcucac cguccaguuc cugauccuug      120

ggaugcugcu caugacagga ggcguuacgc ggcgcgggag cgccuacuau auguaccugg      180

auagaaacga cgcuggagag gccauuuccu uucccaccac acuuggcaug aauaaaugcu      240

auauccaaau uauggaucug gggcauaugu gugacgcgac caugucuuac gaaugcccua      300

ugcucgauga gggaguggaa ccggacgaug uggacuguug gugcaacaca accagcacau      360

gggucgugua uggcaccugu caccauaaga aaggcgaggc ucggcgcucc agacgggccg      420

uuacgcugcc cucucacagc acccgcaagc ugcagacaag aucccagacc uggcucgaaa      480

gccgggagua cacaaaacau cugauccgcg uggagaauug gauuuucaga aacccugggu      540

uugcccuugc ugccgcugcc aucgcguggc ugcucggauc uuccaccagc cagaagguca      600

ucuaucuggu gaugauucuu cugaucgccc ccgcuuacuc uauucggugc aucggcguuu      660

ccaaucgcga uuucguggaa gggaugagcg gaggcacgug ggucgacauu gugcucgagc      720

acgggggaug ugugacaguc auggcccaag auaagccuac cguugacauc gaacugguga      780

caaccaccgu gucuaacaug gcugagguca gauccuacug cuaugaggcc agcauuuccg      840

auauggcguc ugacagccgg ugucccacac agggcgaagc cuaccuggac aaacaguccg      900

auacgcagua ugugugcaag agaacccucg uugaccgcgg cugggggaau ggauguggcc      960

uguuugggaa aggaagccuu gugacaugcg cuaaguucgc cugcucuaaa aagaugaccg     1020

gcaaguccau ccaaccugag aaccuggaau accggaucau gcucagcguc cacgggucuc     1080

agcauagcgg aaugauugug aaugauacag gccacgagac cgacgaaaac agagcuaaag     1140

uugagaucac gcccaauucc ccgcgcgccg aggccacacu gggcggguuu ggaucucuug     1200

gccuggauug ugaaccucgg accgggcucg acuucagcga ucuguauuac cugaccauga     1260

acaauaagca uuggcucgug cacaaagagu gguuucacga cauuccccug ccuuggcaug     1320

cuggagcgga uacaggcaca ccccacugga acaauaagga agcccuuguc gaguucaaag     1380

acgcucaugc caagagacag acaguggucg ugcugggguc ccaggaaggc gccguucaca     1440

cggcucucgc cggagcgcug gaggcugaga uggauggcgc caaagggcgc cuuagcucug     1500

gacaucugaa gugccggcuc aagauggaca aacugagacu gaagggcgug uccuauagcc     1560

ucuguaccgc cgcuuuuaca uucaccaaaa ucccggccga aacacugcac gggaccguca     1620

cgguggaggu ucaauacgcu ggaaccgacg gcccuugcaa ggugcccgcc cagauggcgg     1680

ucgauaugca gacacuuacc ccuguggggc gccugauuac agccaacccc gugaucaccg     1740

aaucuacgga gaacuccaaa augaugcucg aacuggaucc cccuuuuggc gacagcuaca     1800

ucguuauugg agucggcgag aagaagauca cacaccauug gcaccggucc gggucuacca     1860

uuggaaaggc uuucgaggcc acagugagag gcgcuaaacg cauggccgug cugggggaca     1920

ccgcguggga uuucggaagc gucggcgggg cccuuaauuc ccucggcaaa ggaauccauc     1980

aaauuuuugg cgcugccuuc aagucucugu uugggggaau gagcugguuc ucccagaucc     2040

ugaucggcac ccuccugaug uggcuugggc ugaacaccaa aaauggaagc auuucucuca     2100

ugugucuggc ucugggcggg gugcuuaucu uucucuccac ggccguuagc gccgacgugg     2160

gcugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6667
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6667
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc ccggaagcgc agaucucacg acgugcucac cguccaguuc cugauccuug      120

ggaugcugcu caugacagga gccguuacgc ggcgcggcag cgcuuacuau auguaccugg      180

auagaaacga cgccggggag gcgauuuccu uucccaccac acuuggaaug aauaaaugcu      240

auauccaaau uauggaucug ggccauaugu gugacgcuac caugucuuac gaaugcccua      300

ugcucgauga ggggguggaa ccggacgaug uggacuguug gugcaacaca accagcacau      360

gggucgugua uggaaccugu caccauaaga aaggcgaggc ccggcgcucc agacgggccg      420

uuacgcugcc cucucacagc acccgcaagc ugcagacaag aucccagacc uggcucgaaa      480

gccgggagua cacaaaacau cugauccgcg uggagaauug gauuuucaga aacccuggcu      540

uugcucuugc cgcugccgcg aucgccuggc ugcucggguc uuccaccagc cagaagguca      600

ucuaucuggu gaugauucuu cugaucgcuc ccgccuacuc uauucggugc aucggaguuu      660

ccaaucgcga uuucguggaa ggcaugagcg ggggaacgug ggucgacauu gugcucgagc      720

acggcgggug ugugacaguc auggcucaag auaagccuac cguugacauc gaacugguga      780

caaccaccgu gucuaacaug gccgagguca gauccuacug cuaugaggcg agcauuuccg      840

auauggccuc ugacagccgg ugucccacac agggagaggc uuaccuggac aaacaguccg      900

auacgcagua ugugugcaag agaacccucg uugaccgcgg cuggggcaau ggguguggac      960

uguuuggcaa agggagccuu gugacaugcg ccaaguucgc uugcucuaaa aagaugaccg     1020

gaaaguccau ccaaccugaa aaccuggaau accggaucau gcucagcguc cacggcucuc     1080

agcauagcgg gaugauugug aaugauacag gacacgagac cgacgaaaac agagccaaag     1140

uugagaucac gcccaauucc ccgcgcgccg aggcuacacu gggcggcuuu gggucucuug     1200

gacuggauug ugaaccucgg accggccucg acuucagcga ucuguauuac cugaccauga     1260

acaauaagca uuggcucgug cacaaagagu gguuucacga cauuccccug ccuuggcaug     1320

cgggggccga uacaggaaca ccccacugga acaauaagga agcucuuguc gaguucaaag     1380

acgcccaugc caagagacag acaguggucg ugcugggcuc ccaggaaggg gcuguucaca     1440

cggcccucgc gggcgcucug gaggccgaga uggauggagc caaaggccgc cuuagcucug     1500

ggcaucugaa gugccggcuc aagauggaca aacugagacu gaagggagug uccuauagcc     1560

ucuguaccgc ugccuuuaca uucaccaaaa ucccggcuga aacacugcac ggcaccguca     1620

cgguggaggu ucaauacgcc gggaccgacg gaccuugcaa ggugcccgcg cagauggccg     1680

ucgauaugca gacacuuacc ccugugggcc gccugauuac agcuaacccc gugaucaccg     1740

aaucuacgga gaacuccaaa augaugcucg aacuggaucc cccuuuuggg gacagcuaca     1800

ucguuauugg cgucggagag aagaagauca cacaccauug gcaccggucc ggcucuacca     1860

uugggaaagc cuucgaggcu acagugagag gagccaagcg cauggcggug cugggcgaca     1920

ccgccuggga uuucgggagc gucggaggcg cucuuaauuc ccucgggaaa ggcauccauc     1980

aaauuuuugg agccgcuuuc aagucucugu uuggcgggau gagcugguuc ucccagaucc     2040

ugaucggaac ccuccugaug uggcuuggcc ugaacaccaa aaaugggagc auuucucuca     2100

ugugucuggc ccugggaggc gugcuuaucu uucucuccac ggccguuagc gcugacgugg     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6668
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6668
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacauug      720

ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca guugacaucg      780

agcuugugac caccacagug uccaauaugg ccgaagucag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ugaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcgcacgcu aagagacaga cgguggucgu gcucggcucu caggaggggg     1440

ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

ugauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc gcuguuucug     2160

ccgacugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6669
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6669
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uuaacgccag aaaggagaaa aagcggcgcg gcgcugacac cagcgugggg aucgucggac      120

ugcuccugac aacggccaug gcggcugaag uuaccagacg gggcuccgcc uacuauaugu      180

accuugaucg caaugacgcc ggggaggcua uuucuuuccc cacaacccug ggaaugaaca      240

aaugcuauau ccagauuaug gaucucggcc acauguguga cgccacaaug agcuacgaau      300

gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc aauaccacau      360

ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggcuaga cggucucgca      420

gagccguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu cagacauggc      480

ucgagagcag agaauacacc aagcaccuga uccgggugga gaacuggauc uuucgcaauc      540

cuggcuucgc gcuggccgcu gccgcuauug ccuggcuccu gggguccagc acgucucaga      600

aagucaucua ucuugugaug auucugcuca ucgcgcccgc cuacuccauu agaugcaucg      660

gaguuagcaa ccgggauuuu guggaaggca ugucuggggg aacauggguc gacauugugc      720

uggagcaugg cgggugugug accgucaugg cucaggauaa gccuacaguu gacaucgagc      780

uugugaccac cacagugucc aauauggccg aaguccgcag cuacugcuau gaggcuucua      840

ucuccgauau ggccagcgac uccagauguc ccacgcaagg agaagccuac cuggacaaac      900

agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg ggcaacgggu      960

guggacuguu cggcaaaggg agccugguga ccugcgcuaa guuugcgugc uccaagaaaa     1020

ugacaggaaa gagcauucag ccugagaauc ucgaauaccg caucaugcug ucuguccacg     1080

gcucccaaca cagcgggaug auugugaacg auaccggaca ugagacggac gagaaucggg     1140

ccaaaguuga aaucacaccg aacucuccca gagcugaggc cacccuuggc ggcuucggga     1200

gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu uauuaccuga     1260

caaugaauaa caagcacugg cucgugcaua aagagugguu ccacgacauu ccccugccuu     1320

ggcacgccgg ggcugauacc ggaacacccc auuggaauaa caaggaagcc cuggucgagu     1380

uuaaagacgc gcacgcuaag cggcagacgg uggucgugcu cggcucucag gagggggccg     1440

uucauaccgc ccuggcuggc gcccuugaag cugagaugga uggagccaag ggcagacuga     1500

gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag ggagugagcu     1560

auucucucug uacagcggcc uucaccuuua caaaaauccc ggcugaaacc cugcauggca     1620

cggucaccgu ggagguucag uacgccggga cagacggacc uugcaaggug cccgcucaaa     1680

uggccgucga uaugcagacc cugacaccug ugggcagacu caucaccgcg aaccccguga     1740

uuacggaauc cacagagaau agcaagauga ugcuggagcu ugaucccccu uucggggacu     1800

cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau cgcuccggca     1860

gcacaauugg gaaagccuuu gaagcuaccg ugcggggagc caagagaaug gcugugcugg     1920

gcgacaccgc cugggauuuc ggguccgucg gaggcgcccu caacucucug gggaaaggca     1980

uccaccagau cuucggagcu gcguuuaaga gccuguucgg cgggaugucc ugguuuucuc     2040

aaauucuuau cggaacacuc cugauguggc ugggccucaa uacgaagaac gggagcauuu     2100

cccugaugug ucuugcccug ggaggcgugc ucaucuuccu gagcaccgcu guuucugccg     2160

acugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6670
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6670
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaaaaag cggcgcggcg cugacaccag cguggggauu gucggacugc      120

uccugacaac ggccauggcg gcugaaguua ccagacgggg cuccgccuac uauauguacc      180

uugaucgcaa ugacgccggg gaggcuaucu cuuuccccac aacccuggga augaacaaau      240

gcuauauuca gaucauggau cucggccaca ugugugacgc cacaaugagc uacgaaugcc      300

cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau accacaucca      360

ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcuagacgg ucucgcagag      420

ccguuacccu ucccagccau uccacacgga agcugcaaac ccgcucucag acauggcucg      480

agagcagaga auacaccaag caccugauuc ggguggagaa cuggaucuuu cgcaauccug      540

gcuucgcgcu ggccgcugcc gcuaucgccu ggcuccuggg guccagcacg ucucagaaag      600

ucauuuaucu ugugaugauc cugcucauug cgcccgccua cuccaucaga ugcauuggag      660

uuagcaaccg ggauuuugug gaaggcaugu cugggggaac augggucgac aucgugcugg      720

agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac auugagcuug      780

ugaccaccac aguguccaau auggccgaag uccgcagcua cugcuaugag gcuucuaucu      840

ccgauauggc cagcgacucc agauguccca cgcaaggaga agccuaccug gacaaacagu      900

cugauaccca guaugugugc aagcggacac ucguugacag aggcuggggc aacgggugug      960

gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc aagaaaauga     1020

caggaaagag cauccagccu gagaaucucg aauaccgcau uaugcugucu guccacggcu     1080

cccaacacag cgggaugauc gugaacgaua ccggacauga gacggacgag aaucgggcca     1140

aaguugaaau uacaccgaac ucucccagag cugaggccac ccuuggcggc uucgggagcc     1200

ugggacucga uugugaaccu cgcaccggcc uggacuuuuc cgaucuuuau uaccugacaa     1260

ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacaucccc cugccuuggc     1320

acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug gucgaguuua     1380

aagacgcgca cgcuaagcgg cagacggugg ucgugcucgg cucucaggag ggggccguuc     1440

auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc agacugagcu     1500

ccgggcaccu caaaugccgc cugaagaugg acaaacuucg gcugaaggga gugagcuauu     1560

cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug cauggcacgg     1620

ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc gcucaaaugg     1680

ccgucgauau gcagacccug acaccugugg gcagacucau caccgcgaac cccgugauca     1740

cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc ggggacucuu     1800

acauuguuau cggcgucgga gagaaaaaga uuacccacca cuggcaucgc uccggcagca     1860

caaucgggaa agccuuugaa gcuaccgugc ggggagccaa gagaauggcu gugcugggcg     1920

acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg aaaggcauuc     1980

accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg uuuucucaaa     2040

uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg agcaucuccc     2100

ugaugugucu ugcccuggga ggcgugcuca uuuuccugag caccgcuguu ucugccgacu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6671
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6671
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaaaaagcgg cgcggcgcug acaccagcgu ggggaucguc ggacugcucc      120

ugacaacggc cauggcggcu gaaguuacca gacggggcuc cgccuacuau auguaccuug      180

aucgcaauga cgccggggag gcuauuucuu uccccacaac ccugggaaug aacaaaugcu      240

auauccagau uauggaucuc ggccacaugu gugacgccac aaugagcuac gaaugcccua      300

ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaauacc acauccaccu      360

gggucgugua uggaacgugu caucacaaga aaggcgaggc uagacggucu cgcagagccg      420

uuacccuucc cagccauucc acacggaagc ugcaaacccg cucucagaca uggcucgaga      480

gcagagaaua caccaagcac cugauccggg uggagaacug gauuuuucgc aauccuggcu      540

ucgcgcuggc cgcugccgcu aucgccuggc uccugggguc cagcacgucu cagaaaguca      600

ucuaucuugu gaugauucug cucaucgcgc ccgccuacuc cauuagaugc aucggaguua      660

gcaaccggga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu gugcuggagc      720

auggcgggug ugugaccguc auggcucagg auaagccuac aguugacauc gagcuuguga      780

ccaccacagu guccaauaug gccgaagucc gcagcuacug cuaugaggcu ucuauuuccg      840

auauggccag cgacuccaga ugucccacgc aaggagaagc cuaccuggac aaacagucug      900

auacccagua ugugugcaag cggacacucg uugacagagg cuggggcaac ggguguggac      960

uguucggcaa agggagccug gugaccugcg cuaaguuugc gugcuccaag aaaaugacag     1020

gaaagagcau ccagccugag aaucucgaau accgcaucau gcugucuguc cacggcuccc     1080

aacacagcgg gaugauugug aacgauaccg gacaugagac ggacgagaau cgggccaaag     1140

uugaaaucac accgaacucu cccagagcug aggccacccu uggcggcuuc gggagccugg     1200

gacucgauug ugaaccucgc accggccugg acuuuuccga ucuuuauuac cugacaauga     1260

auaacaagca cuggcucgug cauaaagagu gguuccacga cauuccccug ccuuggcacg     1320

ccggggcuga uaccggaaca ccccauugga auaacaagga agcccugguc gaguuuaaag     1380

acgcgcacgc uaagcggcag acgguggucg ugcucggcuc ucaggagggg gccguucaua     1440

ccgcccuggc uggcgcccuu gaagcugaga uggauggagc caagggcaga cugagcuccg     1500

ggcaccucaa augccgccug aagauggaca aacuucggcu gaagggagug agcuauucuc     1560

ucuguacagc ggccuucacc uuuacaaaaa ucccggcuga aacccugcau ggcacgguca     1620

ccguggaggu ucaguacgcc gggacagacg gaccuugcaa ggugcccgcu caaauggccg     1680

ucgauaugca gacccugaca ccugugggca gacucauuac cgcgaacccc gugaucacgg     1740

aauccacaga gaauagcaag augaugcugg agcuugaucc cccuuucggg gacucuuaca     1800

ucguuauugg cgucggagag aaaaagauca cccaccacug gcaucgcucc ggcagcacaa     1860

uugggaaagc cuuugaagcu accgugcggg gagccaagag aauggcugug cugggcgaca     1920

ccgccuggga uuucgggucc gucggaggcg cccucaacuc ucuggggaaa ggcauccacc     1980

agauuuucgg agcugcguuu aagagccugu ucggcgggau guccugguuu ucucaaaucc     2040

uuaucggaac acuccugaug uggcugggcc ucaauacgaa gaacgggagc auuucccuga     2100

ugugucuugc ccugggaggc gugcucaucu uccugagcac cgcuguuucu gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6672
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6672
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa aaagcggcgc ggcgcugaca ccagcguggg gaucgucgga cugcuccuga      120

caacggccau ggcggcugaa guuaccagac ggggcuccgc cuacuauaug uaccuugauc      180

gcaacgacgc cggggaggcu auuucuuucc ccacaacccu gggaaugaau aaaugcuaua      240

uccagauuau ggaucucggc cacaugugug acgccacaau gagcuacgaa ugcccuaugc      300

uggaugaggg gguggaaccg gacgaugugg acuguuggug caacaccaca uccaccuggg      360

ucguguaugg aacgugucau cacaagaaag gcgaggcuag acggucucgc agagccguua      420

cccuucccag ccauuccaca cggaagcugc aaacccgcuc ucagacaugg cucgagagca      480

gagaauacac caagcaccug auccgggugg agaauuggau uuuucgcaac ccuggcuucg      540

cgcuggccgc ugccgcuauc gccuggcucc ugggguccag cacgucucag aaagucaucu      600

aucuugugau gauucugcuc aucgcgcccg ccuacuccau uagaugcauc ggaguuagca      660

aucgggauuu uguggaaggc augucugggg gaacaugggu cgacauugug cuggagcaug      720

gcgggugugu gaccgucaug gcucaggaua agccuacagu ugacaucgag cuugugacca      780

ccacaguguc caacauggcc gaaguccgca gcuacugcua ugaggcuucu auuuccgaua      840

uggccagcga cuccagaugu cccacgcaag gagaagccua ccuggacaaa cagucugaua      900

cccaguaugu gugcaagcgg acacucguug acagaggcug gggcaauggg uguggacugu      960

ucggcaaagg gagccuggug accugcgcua aguuugcgug cuccaagaaa augacaggaa     1020

agagcaucca gccugagaac cucgaauacc gcaucaugcu gucuguccac ggcucccaac     1080

acagcgggau gauugugaau gauaccggac augagacgga cgagaaccgg gccaaaguug     1140

aaaucacacc caauucuccc agagcugagg ccacccuugg cggcuucggg agccugggac     1200

ucgauuguga accucgcacc ggccuggacu uuuccgaucu uuauuaccug acaaugaaca     1260

auaagcacug gcucgugcau aaagaguggu uccacgacau uccccugccu uggcacgccg     1320

gggcugauac cggaacaccc cauuggaaca auaaggaagc ccuggucgag uuuaaagacg     1380

cgcacgcuaa gcggcagacg guggucgugc ucggcucuca ggagggggcc guucauaccg     1440

cccuggcugg cgcccuugaa gcugagaugg auggagccaa gggcagacug agcuccgggc     1500

accucaaaug ccgccugaag auggacaaac uucggcugaa gggagugagc uauucucucu     1560

guacagcggc cuucaccuuu acaaaaaucc cggcugaaac ccugcauggc acggucaccg     1620

uggagguuca guacgccggg acagacggac cuugcaaggu gcccgcucaa auggccgucg     1680

auaugcagac ccugacaccu gugggcagac ucauuaccgc gaaccccgug aucacggaau     1740

ccacagagaa cagcaagaug augcuggagc uugacccgcc uuucggggau ucuuacaucg     1800

uuauuggcgu cggagagaaa aagaucaccc accacuggca ucgcuccggc agcacaauug     1860

ggaaagccuu ugaagcuacc gugcggggag ccaagagaau ggcugugcug ggcgacaccg     1920

ccugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaaaggc auccaccaga     1980

uuuucggagc ugcguuuaag agccuguucg gcgggauguc cugguuuucu caaauccuua     2040

ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu ucccugaugu     2100

gucuugcccu gggaggcgug cucaucuucc ugagcaccgc uguuucugcc gacugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6673
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6673
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaaaaa gcggcgcggc gccgacacca gcguggggau cgucggacug cuccugacaa      120

cggcuauggc cgcggaaguu accagacggg gcuccgcuua cuauauguac cuugaucgca      180

acgacgccgg ggaggccauu ucuuucccca caacccuggg aaugaauaaa ugcuauaucc      240

agauuaugga ucucggccac augugugacg cuacaaugag cuacgaaugc ccuaugcugg      300

augagggggu ggaaccggac gauguggacu guuggugcaa caccacaucc accugggucg      360

uguauggaac gugucaucac aagaaaggcg aggccagacg gucucgcaga gcuguuaccc      420

uucccagcca uuccacacgg aagcugcaaa cccgcucuca gacauggcuc gagagcagag      480

aauacaccaa gcaccugauc cggguggaga auuggauuuu ucgcaacccu ggcuucgccc      540

uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaaa gucaucuauc      600

uugugaugau ucugcucauc gcccccgcgu acuccauuag augcaucgga guuagcaauc      660

gggauuuugu ggaaggcaug ucugggggaa caugggucga cauugugcug gagcauggcg      720

ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu gugaccacca      780

caguguccaa cauggcugaa guccgcagcu acugcuauga ggccucuauu uccgauaugg      840

cuagcgacuc cagauguccc acgcaaggag aagccuaccu ggacaaacag ucugauaccc      900

aguaugugug caagcggaca cucguugaca gaggcugggg caaugggugu ggacuguucg      960

gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caagaaaaug acaggaaaga     1020

gcauccagcc ugagaaccuc gaauaccgca ucaugcuguc uguccacggc ucccaacaca     1080

gcgggaugau ugugaaugau accggacaug agacggacga gaaccgggcg aaaguugaaa     1140

ucacacccaa uucucccaga gccgaggcua cccuuggcgg cuucgggagc cugggacucg     1200

auugugaacc ucgcaccggc cuggacuuuu ccgaucuuua uuaccugaca augaacaaua     1260

agcacuggcu cgugcauaaa gagugguucc acgacauucc ccugccuugg cacgccgggg     1320

ccgauaccgg aacaccccau uggaacaaua aggaagcucu ggucgaguuu aaagacgccc     1380

acgcgaagcg gcagacggug gucgugcucg gcucucagga gggggcuguu cauaccgccc     1440

uggccggcgc ucuugaagcc gagauggaug gagcuaaggg cagacugagc uccgggcacc     1500

ucaaaugccg ccugaagaug gacaaacuuc ggcugaaggg agugagcuau ucucucugua     1560

cagccgcguu caccuuuaca aaaaucccgg ccgaaacccu gcauggcacg gucaccgugg     1620

agguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug gcugucgaua     1680

ugcagacccu gacaccugug ggcagacuca uuaccgccaa ccccgugauc acggaaucca     1740

cagagaacag caagaugaug cuggagcuug acccgccuuu cggggauucu uacaucguua     1800

uuggcgucgg agagaaaaag aucacccacc acuggcaucg cuccggcagc acaauuggga     1860

aagcguuuga agccaccgug cggggagcua agagaauggc cgugcugggc gacaccgcuu     1920

gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc caccagauuu     1980

ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa auccuuaucg     2040

gaacacuccu gauguggcug ggccucaaca cgaagaaugg gagcauuucc cugauguguc     2100

uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6674
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6674
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaaaaagag acggggcgcc gacaccagcg uggggaucgu cggacugcuc cugacaacgg      120

cuauggccgc ggaaguuacc cgcagaggcu ccgcuuacua uauguaccuu gaucggaacg      180

acgccgggga ggccauuucu uuccccacaa cccugggaau gaauaaaugc uauauccaga      240

uuauggaucu cggccacaug ugugacgcua caaugagcua cgaaugcccu augcuggaug      300

agggggugga accggacgau guggacuguu ggugcaacac cacauccacc ugggucgugu      360

auggaacgug ucaucacaag aaaggcgagg cccgcagauc ucggcgcgcu guuacccuuc      420

ccagccauuc cacaagaaag cugcaaaccc ggucucagac auggcucgag agccgcgaau      480

acaccaagca ccugaucaga guggagaauu ggauuuuucg gaacccuggc uucgcccugg      540

cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc aucuaucuug      600

ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu agcaauagag      660

auuuugugga aggcaugucu gggggaacau gggucgacau ugugcuggag cauggcgggu      720

gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug accaccacag      780

uguccaacau ggcugaaguc cggagcuacu gcuaugaggc cucuauuucc gauauggcua      840

gcgacucccg cugucccacg caaggagaag ccuaccugga caaacagucu gauacccagu      900

augugugcaa gagaacacuc guugaccggg gcuggggcaa ugggugugga cuguucggca      960

aagggagccu ggugaccugc gccaaguuug cuugcuccaa gaaaaugaca ggaaagagca     1020

uccagccuga gaaccucgaa uacagaauca ugcugucugu ccacggcucc caacacagcg     1080

ggaugauugu gaaugauacc ggacaugaga cggacgagaa ccgcgcgaaa guugaaauca     1140

cacccaauuc uccccgggcc gaggcuaccc uuggcggcuu cgggagccug ggacucgauu     1200

gugaaccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug aacaauaagc     1260

acuggcucgu gcauaaagag ugguuccacg acauuccccu gccuuggcac gccggggccg     1320

auaccggaac accccauugg aacaauaagg aagcucuggu cgaguuuaaa gacgcccacg     1380

cgaagcgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau accgcccugg     1440

ccggcgcucu ugaagccgag auggauggag cuaagggccg gcugagcucc gggcaccuca     1500

aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu cucuguacag     1560

ccgcguucac cuuuacaaaa aucccggccg aaacccugca uggcacgguc accguggagg     1620

uucaguacgc ugggacagac ggaccuugca aggugcccgc ccaaauggcu gucgauaugc     1680

agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg gaauccacag     1740

agaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac aucguuauug     1800

gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca auugggaaag     1860

cguuugaagc caccgugcgc ggagcuaagc ggauggccgu gcugggcgac accgcuuggg     1920

auuucggguc cgucggaggc gcccucaauu cucuggggaa aggcauccac cagauuuucg     1980

gagccgcuuu uaagagccug uucggcggga uguccugguu uucucaaauc cuuaucggaa     2040

cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug augugucuug     2100

cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6675
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6675
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagacg gggcgccgac accagcgugg ggaucgucgg acugcuccug acaacggcua      120

uggccgcgga aguuacccgc agaggcuccg cuuacuauau guaccuugau cggaacgacg      180

ccggggaggc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau auccagauua      240

uggaucucgg ccacaugugu gacgcuacaa ugagcuacga augcccuaug cuggaugagg      300

ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg gucguguaug      360

gaacguguca ucacaaaaag ggcgaggccc gcagaucucg gcgcgcuguu acccuuccca      420

gccauuccac aagaaaacug caaacccggu cucagacaug gcucgagagc cgcgaauaca      480

ccaagcaccu gaucagagug gagaauugga uuuuucggaa cccuggcuuc gcccuggcgg      540

ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaggucauc uaucuuguga      600

ugauucugcu caucgccccc gcguacucca uucgcugcau cggaguuagc aauagagauu      660

uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau ggcgggugug      720

ugaccgucau ggcccaggau aaaccuacag uugacaucga gcuugugacc accacagugu      780

ccaacauggc ugaaguccgg agcuacugcu augaggccuc uauuuccgau auggcuagcg      840

acucccgcug ucccacgcaa ggagaagccu accuggacaa gcagucugau acccaguaug      900

ugugcaaaag aacacucguu gaccggggcu ggggcaaugg guguggacug uucggcaagg      960

ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa gaugacagga aaaagcaucc     1020

agccugagaa ccucgaauac agaaucaugc ugucugucca cggcucccaa cacagcggga     1080

ugauugugaa ugauaccgga caugagacgg acgagaaccg cgcgaagguu gaaaucacac     1140

ccaauucucc ccgggccgag gcuacccuug gcggcuucgg gagccuggga cucgauugug     1200

aaccuagaac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac aauaaacacu     1260

ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc ggggccgaua     1320

ccggaacacc ccauuggaac aauaaagaag cucuggucga guuuaaggac gcccacgcga     1380

aacgccagac gguggucgug cucggcucuc aggagggggc uguucauacc gcccuggccg     1440

gcgcucuuga agccgagaug gauggagcua agggccggcu gagcuccggg caccucaagu     1500

gcagacugaa aauggacaaa cuucgccuga agggagugag cuauucucuc uguacagccg     1560

cguucaccuu uacaaagauc ccggccgaaa cccugcaugg cacggucacc guggagguuc     1620

aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc gauaugcaga     1680

cccugacacc ugugggccgg cucauuaccg ccaaccccgu gaucacggaa uccacagaga     1740

acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc guuauuggcg     1800

ucggagaaaa gaaaaucacc caccacuggc auagauccgg cagcacaauu gggaaggcgu     1860

uugaggccac cgugcgcgga gcuaaacgga uggccgugcu gggcgacacc gcuugggauu     1920

ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag auuuucggag     1980

ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu aucggaacac     2040

uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug ugucuugcgc     2100

ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6676
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6676
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagacgggg cgccgacacc agcgugggga ucgucggacu gcuccugaca acggcuaugg      120

ccgcggaggu uacccgcaga ggcuccgcuu acuauaugua ccuugaucgg aacgacgccg      180

gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc cagauuaugg      240

aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug gaugaagggg      300

uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc guguauggaa      360

cgugucauca caaaaagggc gaagcccgca gaucucggcg cgcuguuacc cuucccagcc      420

auuccacaag aaaacugcaa acccggucuc agacauggcu cgagagccgc gaguacacca      480

agcaccugau cagaguggaa aauuggauuu uucggaaccc uggcuucgcc cuggcggccg      540

cugccaucgc uuggcuccug ggguccagca cgucucagaa ggucaucuau cuugugauga      600

uucugcucau cgcccccgcg uacuccauuc gcugcaucgg aguuagcaau agagauuuug      660

uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc ggguguguga      720

ccgucauggc ccaggauaaa ccuacaguug acaucgagcu ugugaccacc acagugucca      780

acauggcuga gguccggagc uacugcuaug aagccucuau uuccgauaug gcuagcgacu      840

cccgcugucc cacgcaagga gaggccuacc uggacaagca gucugauacc caguaugugu      900

gcaaaagaac acucguugac cggggcuggg gcaaugggug uggacuguuc ggcaagggga      960

gccuggugac cugcgccaaa uuugcuugcu ccaagaagau gacaggaaaa agcauccagc     1020

cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac agcgggauga     1080

uugugaauga uaccggacau gaaacggacg agaaccgcgc gaagguugag aucacaccca     1140

auucuccccg ggccgaagcu acccuuggcg gcuucgggag ccugggacuc gauugugagc     1200

cuagaaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau aaacacuggc     1260

ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg gccgauaccg     1320

gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc cacgcgaaac     1380

gccagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc cuggccggcg     1440

cucuugaggc cgaaauggau ggagcuaagg gccggcugag cuccgggcac cucaagugca     1500

gacugaaaau ggacaaacuu cgccugaagg gagugagcua uucucucugu acagccgcgu     1560

ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug gaaguucagu     1620

acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau augcagaccc     1680

ugacaccugu gggccggcuc auuaccgcca accccgugau cacggagucc acagaaaaca     1740

gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu auuggcgucg     1800

gagagaagaa aaucacccac cacuggcaua gauccggcag cacaauuggg aaggcguuug     1860

aagccaccgu gcgcggagcu aaacggaugg ccgugcuggg cgacaccgcu ugggauuucg     1920

gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu uucggagccg     1980

cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc ggaacacucc     2040

ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu cuugcgcugg     2100

gaggcgugcu caucuuccug agcaccgccg uuucugcuga cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6677
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6677
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gacggggcgc cgacaccagc guggggaucg ucggacugcu ccugacaacg gcuauggccg      120

cggagguuac ccgcagaggc uccgcuuacu auauguaccu ugaucggaac gacgccgggg      180

aagccauuuc uuuccccaca acccugggaa ugaauaaaug cuauauccag auuauggauc      240

ucggccacau gugugacgcu acaaugagcu acgagugccc uaugcuggau gaaggggugg      300

agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug uauggaacgu      360

gucaucacaa gaaaggcgaa gcccgcagau cucggcgcgc uguuacccuu cccagccauu      420

ccacaagaaa gcugcaaacc cggucucaga cauggcucga gagccgcgag uacaccaaac      480

accugaucag aguggaaaau uggauuuuuc ggaacccugg cuucgcccug gcggccgcug      540

ccaucgcuug gcuccugggg uccagcacgu cucagaaggu caucuaucuu gugaugauuc      600

ugcucaucgc ccccgcguac uccauucgcu gcaucggagu uagcaauaga gauuuugugg      660

agggcauguc ugggggaaca ugggucgaca uugugcugga acauggcggg ugugugaccg      720

ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca guguccaaca      780

uggcugaggu ccggagcuac ugcuaugaag ccucuauuuc cgauauggcu agcgacuccc      840

gcugucccac gcaaggagag gccuaccugg acaaacaguc ugauacccag uaugugugca      900

agagaacacu cguugaccgg ggcuggggca augggugugg acuguucggc aaagggagcc      960

uggugaccug cgccaaguuu gcuugcucca aaaagaugac aggaaagagc auccagccug     1020

aaaaccucga guacagaauc augcugucug uccacggcuc ccaacacagc gggaugauug     1080

ugaaugauac cggacaugaa acggacgaga accgcgcgaa aguugagauc acacccaauu     1140

cuccccgggc cgaagcuacc cuuggcggcu ucgggagccu gggacucgau ugugagccua     1200

gaaccggccu ggacuuuucc gaucuuuauu accugacaau gaacaauaag cacuggcucg     1260

ugcauaaaga augguuccac gacauucccc ugccuuggca cgccggggcc gauaccggaa     1320

caccccauug gaacaauaag gaggcucugg ucgaauuuaa agacgcccac gcgaagcgcc     1380

agacgguggu cgugcucggc ucucaggagg gggcuguuca uaccgcccug gccggcgcuc     1440

uugaggccga aauggaugga gcuaaaggcc ggcugagcuc cgggcaccuc aagugcagac     1500

ugaagaugga caaacuucgc cugaagggag ugagcuauuc ucucuguaca gccgcguuca     1560

ccuuuacaaa aaucccggcc gagacccugc auggcacggu caccguggaa guucaguacg     1620

cugggacaga cggaccuugc aaggugcccg cccaaauggc ugucgauaug cagacccuga     1680

caccuguggg ccggcucauu accgccaacc ccgugaucac ggaguccaca gaaaacagca     1740

aaaugaugcu ggagcuugac ccgccuuucg gggauucuua caucguuauu ggcgucggag     1800

agaagaagau cacccaccac uggcauagau ccggcagcac aauugggaaa gcguuugaag     1860

ccaccgugcg cggagcuaag cggauggccg ugcugggcga caccgcuugg gauuucgggu     1920

ccgucggagg cgcccucaau ucucugggga aaggcaucca ccagauuuuc ggagccgcuu     1980

uuaagagccu guucggcggg auguccuggu uuucucaaau ccuuaucgga acacuccuga     2040

uguggcuggg ccucaacacg aaaaauggga gcauuucccu gaugugucuu gcgcugggag     2100

gcgugcucau cuuccugagc accgccguuu cugcugacug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6678
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6678
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgccga caccagcgug gggaucgucg gacugcuccu gacaacggcu auggccgcgg      120

agguuacccg cagaggcucc gcuuacuaua uguaccuuga ucggaacgac gccggggaag      180

ccauuucuuu ccccacaacc cugggaauga auaagugcua uauccagauu auggaucucg      240

gccacaugug ugacgcuaca augagcuacg agugcccuau gcuggaugaa gggguggagc      300

cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau ggaacguguc      360

aucacaaaaa gggcgaagcc cgcagaucuc ggcgcgcugu uacccuuccc agccauucca      420

caagaaaacu gcaaacccgg ucucagacau ggcucgagag ccgcgaguac accaagcacc      480

ugaucagagu ggaaaauugg auuuuucgga acccuggcuu cgcccuggcg gccgcugcca      540

ucgcuuggcu ccuggggucc agcacgucuc agaaagucau cuaucuugug augauucugc      600

ucaucgcccc cgcguacucc auucgcugca ucggaguuag caauagagau uuuguggagg      660

gcaugucugg gggaacaugg gucgacauug ugcuggaaca uggcgggugu gugaccguca      720

uggcccagga uaagccuaca guugacaucg agcuugugac caccacagug uccaacaugg      780

cugagguccg gagcuacugc uaugaagccu cuauuuccga uauggcuagc gacucccgcu      840

gucccacgca aggagaggcc uaccuggaca agcagucuga uacccaguau gugugcaaaa      900

gaacacucgu ugaccggggc uggggcaaug gguguggacu guucggcaag gggagccugg      960

ugaccugcgc caaauuugcu ugcuccaaga aaaugacagg aaagagcauc cagccugaaa     1020

accucgagua cagaaucaug cugucugucc acggcuccca acacagcggg augauuguga     1080

augauaccgg acaugaaacg gacgagaacc gcgcgaaggu ugagaucaca cccaauucuc     1140

cccgggccga agcuacccuu ggcggcuucg ggagccuggg acucgauugu gagccuagaa     1200

ccggccugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac uggcucgugc     1260

auaaggaaug guuccacgac auuccccugc cuuggcacgc cggggccgau accggaacac     1320

cccauuggaa caauaaagag gcucuggucg aauuuaagga cgcccacgcg aaacgccaga     1380

cgguggucgu gcucggcucu caggaggggg cuguucauac cgcccuggcc ggcgcucuug     1440

aggccgaaau ggauggagcu aagggccggc ugagcuccgg gcaccucaaa ugcagacuga     1500

agauggacaa acuucgccug aagggaguga gcuauucucu cuguacagcc gcguucaccu     1560

uuacaaagau cccggccgag acccugcaug gcacggucac cguggaaguu caguacgcug     1620

ggacagacgg accuugcaaa gugcccgccc aaauggcugu cgauaugcag acccugacac     1680

cugugggccg gcucauuacc gccaaccccg ugaucacgga guccacagaa aacagcaaga     1740

ugaugcugga gcuugacccg ccuuucgggg auucuuacau cguuauuggc gucggagaga     1800

aaaagaucac ccaccacugg cauagauccg gcagcacaau ugggaaggcg uuugaagcca     1860

ccgugcgcgg agcuaaacgg auggccgugc ugggcgacac cgcuugggau uucggguccg     1920

ucggaggcgc ccucaauucu cuggggaagg gcauccacca gauuuucgga gccgcuuuua     1980

aaagccuguu cggcgggaug uccugguuuu cucaaauccu uaucggaaca cuccugaugu     2040

ggcugggccu caacacgaag aaugggagca uuucccugau gugucuugcg cugggaggcg     2100

ugcucaucuu ccugagcacc gccguuucug cugacugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6679
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6679
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug gccgcggagg      120

uuacccggcg cggcuccgcu uacuauaugu accuugauag aaacgacgcc ggggaagcca      180

uuucuuuccc cacaacccug ggaaugaaua agugcuauau ccagauuaug gaucucggcc      240

acauguguga cgcuacaaug agcuacgagu gcccuaugcu ggaugaaggg guggagccgg      300

acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga acgugucauc      360

acaaaaaggg cgaagcccgg cgcucuagac gggcuguuac ccuucccagc cauuccacac      420

gcaaacugca aaccagaucu cagacauggc ucgagagccg ggaguacacc aagcaccuga      480

uccgcgugga aaauuggauu uuuagaaacc cuggcuucgc ccuggcggcc gcugccaucg      540

cuuggcuccu gggguccagc acgucucaga aagucaucua ucuugugaug auucugcuca      600

ucgcccccgc guacuccauu cggugcaucg gaguuagcaa ucgcgauuuu guggagggca      660

ugucuggggg aacauggguc gacauugugc uggaacaugg cgggugugug accgucaugg      720

cccaggauaa gccuacaguu gacaucgagc uugugaccac cacagugucc aacauggcug      780

aggucagaag cuacugcuau gaagccucua uuuccgauau ggcuagcgac ucccgguguc      840

ccacgcaagg agaggccuac cuggacaagc agucugauac ccaguaugug ugcaaacgca      900

cacucguuga cagaggcugg ggcaaugggu guggacuguu cggcaagggg agccugguga      960

ccugcgccaa auuugcuugc uccaagaaaa ugacaggaaa gagcauccag ccugaaaacc     1020

ucgaguaccg gaucaugcug ucuguccacg gcucccaaca cagcgggaug auugugaaug     1080

auaccggaca ugaaacggac gagaacagag cgaagguuga gaucacaccc aauucucccc     1140

gcgccgaagc uacccuuggc ggcuucggga gccugggacu cgauugugag ccucggaccg     1200

gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaaacacugg cucgugcaua     1260

aggaaugguu ccacgacauu ccccugccuu ggcacgccgg ggccgauacc ggaacacccc     1320

auuggaacaa uaaagaggcu cuggucgaau uuaaggacgc ccacgcgaaa agacagacgg     1380

uggucgugcu cggcucucag gagggggcug uucauaccgc ccuggccggc gcucuugagg     1440

ccgaaaugga uggagcuaag ggccgccuga gcuccgggca ccucaaaugc cggcugaaga     1500

uggacaaacu uagacugaag ggagugagcu auucucucug uacagccgcg uucaccuuua     1560

caaagauccc ggccgagacc cugcauggca cggucaccgu ggaaguucag uacgcuggga     1620

cagacggacc uugcaaagug cccgcccaaa uggcugucga uaugcagacc cugacaccug     1680

ugggccgccu cauuaccgcc aaccccguga ucacggaguc cacagaaaac agcaagauga     1740

ugcuggagcu ugacccgccu uucggggauu cuuacaucgu uauuggcguc ggagagaaaa     1800

agaucaccca ccacuggcau cgguccggca gcacaauugg gaaggcguuu gaagccaccg     1860

ugagaggagc uaaacgcaug gccgugcugg gcgacaccgc uugggauuuc ggguccgucg     1920

gaggcgcccu caauucucug gggaagggca uccaccagau uuucggagcc gcuuuuaaaa     1980

gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc cugauguggc     2040

ugggccucaa cacgaagaau gggagcauuu cccugaugug ucuugcgcug ggaggcgugc     2100

ucaucuuccu gagcaccgcc guuucugcug acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6680
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6680
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gucggacugc uccugacaac ggcuauggcc gcggagguua      120

ccagacgggg cuccgcuuac uauauguacc uugaucgcaa cgacgccggg gaagccauuu      180

cuuuccccac aacccuggga augaauaagu gcuauaucca gauuauggau cucggccaca      240

ugugugacgc uacaaugagc uacgagugcc cuaugcugga ugaaggggug gagccggacg      300

auguggacug uuggugcaac accacaucca ccugggucgu guauggaacg ugucaucaca      360

aaaagggcga agccagacgg ucucgcagag cuguuacccu ucccagccau uccacacgga      420

aacugcaaac ccgcucucag acauggcucg agagcagaga guacaccaag caccugaucc      480

ggguggaaaa uuggauuuuu cgcaacccug gcuucgcccu ggcggccgcu gccaucgcuu      540

ggcuccuggg guccagcacg ucucagaaag ucaucuaucu ugugaugauu cugcucaucg      600

cccccgcgua cuccauuaga ugcaucggag uuagcaaucg ggauuuugug gagggcaugu      660

cugggggaac augggucgac auugugcugg aacauggcgg gugugugacc gucauggccc      720

aggauaagcc uacaguugac aucgagcuug ugaccaccac aguguccaac auggcugagg      780

uccgcagcua cugcuaugaa gccucuauuu ccgauauggc uagcgacucc agauguccca      840

cgcaaggaga ggccuaccug gacaagcagu cugauaccca guaugugugc aaacggacac      900

ucguugaccg cggcuggggc aaugggugug gacuguucgg caaggggagc cuggugaccu      960

gcgccaaauu ugcuugcucc aagaaaauga caggaaagag cauccagccu gaaaaccucg     1020

aguacagaau caugcugucu guccacggcu cccaacacag cgggaugauu gugaaugaua     1080

ccggacauga aacggacgag aaccgggcga agguugagau cacacccaau ucucccagag     1140

ccgaagcuac ccuuggcggc uucgggagcc ugggacucga uugugagccu cgcaccggcc     1200

uggacuuuuc cgaucuuuau uaccugacaa ugaacaauaa acacuggcuc gugcauaagg     1260

aaugguucca cgacauuccc cugccuuggc acgccggggc cgauaccgga acaccccauu     1320

ggaacaauaa agaggcucug gucgaauuua aggacgccca cgcgaaacgg cagacggugg     1380

ucgugcucgg cucucaggag ggggcuguuc auaccgcccu ggccggcgcu cuugaggccg     1440

aaauggaugg agcuaagggc agacugagcu ccgggcaccu caaaugccgc cugaagaugg     1500

acaaacuucg gcugaaggga gugagcuauu cucucuguac agccgcguuc accuuuacaa     1560

agaucccggc cgagacccug cauggcacgg ucaccgugga aguucaguac gcugggacag     1620

acggaccuug caaagugccc gcccaaaugg cugucgauau gcagacccug acaccugugg     1680

gcagacucau uaccgccaac cccgugauca cggaguccac agaaaacagc aagaugaugc     1740

uggagcuuga cccgccuuuc ggggauucuu acaucguuau uggcgucgga gagaaaaaga     1800

ucacccacca cuggcaucgc uccggcagca caauugggaa ggcguuugaa gccaccgugc     1860

ggggagcuaa aagaauggcc gugcugggcg acaccgcuug ggauuucggg uccgucggag     1920

gcgcccucaa uucucugggg aagggcaucc accagauuuu cggagccgcu uuuaaaagcc     1980

uguucggcgg gauguccugg uuuucucaaa uccuuaucgg aacacuccug auguggcugg     2040

gccucaacac gaagaauggg agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca     2100

ucuuccugag caccgccguu ucugcugacu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6681
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6681
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucguc gggcugcucc ugacaacggc uauggccgcg gagguuacca      120

gacggggauc cgcuuacuau auguaccuug aucgcaacga cgccggcgaa gccauuucuu      180

uccccacaac ccuggggaug aauaagugcu auauccagau uauggaucuc ggacacaugu      240

gugacgcuac aaugagcuac gagugcccua ugcuggauga aggcguggag ccggacgaug      300

uggacuguug gugcaacacc acauccaccu gggucgugua ugggacgugu caucacaaaa      360

agggagaagc cagacggucu cgcagagcug uuacccuucc cagccauucc acacggaaac      420

ugcaaacccg cucucagaca uggcucgaga gcagagagua caccaagcac cugauccggg      480

uggaaaauug gauuuuucgc aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc      540

uccugggcuc cagcacgucu cagaaaguca ucuaucuugu gaugauucug cucaucgccc      600

ccgcguacuc cauuagaugc aucgggguua gcaaucggga uuuuguggag ggaaugucug      660

gcgggacaug ggucgacauu gugcuggaac auggaggcug ugugaccguc auggcccagg      720

auaagccuac aguugacauc gagcuuguga ccaccacagu guccaacaug gcugaggucc      780

gcagcuacug cuaugaagcc ucuauuuccg auauggcuag cgacuccaga ugucccacgc      840

aaggggaggc cuaccuggac aagcagucug auacccagua ugugugcaaa cggacacucg      900

uugaccgcgg auggggcaau ggcugugggc uguucggaaa gggcagccug gugaccugcg      960

ccaaauuugc uugcuccaag aaaaugacag gaaagagcau ccagccugaa aaccucgagu     1020

acagaaucau gcugucuguc cacggguccc aacacagcgg caugauugug aaugauaccg     1080

ggcaugaaac ggacgagaac cgggcgaagg uugagaucac acccaauucu cccagagccg     1140

aagcuacccu uggaggcuuc ggcagccugg ggcucgauug ugagccucgc accggacugg     1200

acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug cauaaggaau     1260

gguuccacga cauuccccug ccuuggcacg ccggcgccga uaccgggaca ccccauugga     1320

acaauaaaga ggcucugguc gaauuuaagg acgcccacgc gaaacggcag acgguggucg     1380

ugcucggauc ucaggagggc gcuguucaua ccgcccuggc cggggcucuu gaggccgaaa     1440

uggauggcgc uaagggaaga cugagcuccg gccaccucaa augccgccug aagauggaca     1500

agcugcggcu gaaaggggug agcuauucuc ucuguacagc cgcguucacc uuuacaaaga     1560

ucccggccga gacccugcau ggaacgguca ccguggaagu ucaguacgcu ggcacagacg     1620

ggccuugcaa agugcccgcc caaauggcug ucgauaugca gacccugaca ccugugggaa     1680

gacucauuac cgccaacccc gugaucacgg aguccacaga aaacagcaag augaugcugg     1740

agcuugaccc gccuuucggc gauucuuaca ucguuauugg ggucggcgag aaaaagauca     1800

cccaccacug gcaucgcucc ggaagcacaa uuggcaaggc guuugaagcc accgugagag     1860

gggcuaaacg gauggccgug cugggagaca ccgcuuggga uuucggcucc gucgggggag     1920

cccucaauuc ucugggcaag gggauccacc agauuuucgg cgccgcuuuu aaaagccugu     1980

ucggaggcau guccugguuu ucucaaaucc uuaucgggac acuccugaug uggcugggac     2040

ucaacacgaa gaauggcagc auuucccuga ugugucuugc gcuuggggga gugcucaucu     2100

uccugagcac cgccguuucu gcugacugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6682
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6682
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgucggg cugcuccuga caacggccau ggcugccgag guuaccagac      120

ggggauccgc guacuauaug uaccuugauc gcaacgacgc uggcgaagcc auuucuuucc      180

ccacaacccu ggggaugaau aagugcuaua uccagauuau ggaucucgga cacaugugug      240

acgccacaau gagcuacgag ugcccuaugc uggaugaagg cguggagccg gacgaugugg      300

acuguuggug caacaccaca uccaccuggg ucguguaugg gacgugucau cacaaaaagg      360

gagaagcuag acggucucgc agagccguua cccuucccag ccauuccaca cggaaacugc      420

aaacccgcuc ucagacaugg cucgagagca gagaguacac caagcaccug auccgggugg      480

aaaauuggau uuuucgcaac ccuggcuucg cucuggccgc ggccgcuauc gccuggcucc      540

ugggcuccag cacgucucag aaagucaucu aucuugugau gauucugcuc aucgcucccg      600

ccuacuccau uagaugcauc gggguuagca aucgggauuu uguggaggga augucuggcg      660

ggacaugggu cgacauugug cuggaacaug gaggcugugu gaccgucaug gcgcaggaua      720

agccuacagu ugacaucgag cuugugacca ccacaguguc caacauggcc gagguccgca      780

gcuacugcua ugaagcuagc auuuccgaua uggccagcga cuccagaugu cccacgcaag      840

gggaggcuua ccuggacaag cagucugaua cccaguaugu gugcaaacgg acacucguug      900

accgcggaug gggcaauggc ugugggcugu ucggaaaggg cagccuggug accugcgcca      960

aauuugccug cuccaagaaa augacaggga agucuaucca gccugaaaac cucgaguaca     1020

gaaucaugcu gucuguccac ggaucccaac acagcggcau gauugugaau gauaccgggc     1080

augaaacgga cgagaaccgg gcuaagguug agaucacacc caauucuccc agagcggaag     1140

ccacccuugg aggcuucggc agccuggggc ucgauuguga gccucgcacc ggacuggacu     1200

uuuccgaucu uuauuaccug acaaugaaca auaaacacug gcucgugcau aaggaauggu     1260

uccacgacau uccccugccu uggcacgcug gcgccgauac cgggacaccc cauuggaaca     1320

auaaagaggc ccuggucgaa uuuaaggacg cucacgccaa acggcagacg guggucgugc     1380

ucggaucuca ggagggcgcg guucauaccg cucuggccgg ggcccuugag gcugaaaugg     1440

auggcgccaa gggaagacug agcuccggcc accucaaaug ccgccugaag auggacaagc     1500

ugcggcugaa aggggugagc uauucucucu guacagcugc cuucaccuuu acaaagaucc     1560

cggcggagac ccugcaugga acggucaccg uggaaguuca guacgccggc acagacgggc     1620

cuugcaaagu gcccgcucaa auggccgucg auaugcagac ccugacaccu gugggaagac     1680

ucauuaccgc uaaccccgug aucacggagu ccacagaaaa cagcaagaug augcuggagc     1740

uugacccgcc uuucggcgau ucuuacaucg uuauuggggu cggcgagaaa aagaucaccc     1800

accacuggca ucgcuccgga agcacaauug gcaaggccuu ugaagcgacc gugagagggg     1860

ccaaacggau ggcugugcug ggagacaccg ccugggauuu cggcuccguc gggggagcuc     1920

ucaauucucu gggcaagggg auccaccaga uuuucggcgc cgccuuuaaa agccuguucg     1980

gaggcauguc cugguuuucu caaauccuua ucgggacacu ccugaugugg cugggacuca     2040

acacgaagaa uggcagcauu ucccugaugu gucuugcucu ugggggagug cucaucuucc     2100

ugagcaccgc gguuucugcc gacugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  6683
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6683
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccgau ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6684
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6684
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgcgguu accagacggg      120

gauccgcuua cuauauguac cuugaccgca acgaugccgg cgaggccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

cuacaaugag cuacgaaugc ccuaugcugg acgagggcgu ggaaccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aggccagacg gucucgcaga gcuguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gaaagcagag aguacaccaa gcaccugauc cggguggaga      480

auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcccccgcgu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggaaggaaug ucuggcggga      660

caugggucga uauugugcug gagcauggag gcugugugac cgucauggcc caggacaagc      720

cuacaguuga uaucgaacuu gugaccacca caguguccaa cauggcugag guccgcagcu      780

acugcuauga ggccucuauu uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aagccuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugcuugcuc caagaaaaug acagggaagu cuauccagcc ugagaaccuc gaauacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

agacggauga aaaccgggcg aagguugaga ucacaccgaa uagccccaga gccgaggcua     1140

cccuuggagg cuucggcagc cuggggcucg acugugaacc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gagugguucc     1260

acgauauucc ccugccuugg cacgccggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaagcucu ggucgaguuu aaggaugccc acgcgaaacg gcagacggug gucgugcucg     1380

gaucucagga aggcgcuguu cauaccgccc uggccggggc ucuugaggcc gagauggacg     1440

gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca aagaucccgg     1560

ccgaaacccu gcauggaacg gucaccgugg agguucagua cgcuggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcugucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgccaa ccccgugauc acggaaucca cagagaacag caagaugaug cuggaacuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggcguuuga ggccaccgug agaggggcua     1860

aacggauggc cgugcuggga gauaccgcuu gggacuucgg cuccgucggg ggagcccuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc aucuuccuga     2100

gcaccgccgu uucugcugau ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6685
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6685
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug gccgcggcug      120

uuacccggcg cggcuccgcc uacuauaugu accuugauag aaacgacgcc ggggaggcua      180

uuucuuuccc cacaacccug ggaaugaaua agugcuauau ccagauuaug gaucucggcc      240

acauguguga cgccacaaug agcuacgaau gcccuaugcu ggaugagggg guggaaccgg      300

acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga acgugucauc      360

acaaaaaggg cgaggcucgg cgcucuagac gggccguuac ccuucccagc cauuccacac      420

gcaaacugca aaccagaucu cagacauggc ucgaaagccg ggaguacacc aagcaccuga      480

uccgcgugga gaauuggauu uuuagaaacc cuggcuucgc gcuggccgcu gccgcuaucg      540

ccuggcuccu gggguccagc acgucucaga aagucaucua ucuugugaug auucugcuca      600

ucgcgcccgc cuacuccauu cggugcaucg gaguuagcaa ucgcgauuuu guggaaggca      660

ugucuggggg aacauggguc gacauugugc uggagcaugg cgggugugug accgucaugg      720

cucaggauaa gccuacaguu gacaucgaac uugugaccac cacagugucc aacauggccg      780

aggucagaag cuacugcuau gaggcuucua uuuccgauau ggccagcgac ucccgguguc      840

ccacgcaagg agaagccuac cuggacaagc agucugauac ccaguaugug ugcaaacgca      900

cacucguuga cagaggcugg ggcaaugggu guggacuguu cggcaagggg agccugguga      960

ccugcgcuaa auuugcgugc uccaagaaaa ugacaggaaa gagcauccag ccugagaacc     1020

ucgaauaccg gaucaugcug ucuguccacg gcucccaaca cagcgggaug auugugaaug     1080

auaccggaca ugagacggac gaaaacagag ccaagguuga gaucacaccc aauucucccc     1140

gcgcugaggc cacccuuggc ggcuucggga gccugggacu cgauugugaa ccucggaccg     1200

gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaaacacugg cucgugcaua     1260

aggagugguu ccacgacauu ccccugccuu ggcacgccgg ggcugauacc ggaacacccc     1320

auuggaacaa uaaagaagcc cuggucgagu uuaaggacgc gcacgcuaaa agacagacgg     1380

uggucgugcu cggcucucag gaaggggccg uucauaccgc ccuggcuggc gcccuugagg     1440

cugagaugga uggagccaag ggccgccuga gcuccgggca ccucaaaugc cggcugaaga     1500

uggacaaacu uagacugaag ggagugagcu auucucucug uacagcggcc uucaccuuua     1560

caaagauccc ggcugaaacc cugcauggca cggucaccgu ggagguucag uacgccggga     1620

cagacggacc uugcaaagug cccgcucaaa uggccgucga uaugcagacc cugacaccug     1680

ugggccgccu cauuaccgcg aaccccguga ucacggaauc cacagagaac agcaagauga     1740

ugcuggaacu ugacccgccu uucggggauu cuuacaucgu uauuggcguc ggagagaaaa     1800

agaucaccca ccacuggcau cgguccggca gcacaauugg gaaggccuuu gaggcuaccg     1860

ugagaggagc caaacgcaug gcugugcugg gcgacaccgc cugggauuuc ggguccgucg     1920

gaggcgcccu caauucucug gggaagggca uccaccagau uuucggagcu gcguuuaaaa     1980

gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc cugauguggc     2040

ugggccucaa cacgaagaau gggagcauuu cccugaugug ucuugcccug ggaggcgugc     2100

ucaucuuccu gagcaccgcu guuucugccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6686
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6686
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc ccggaagcgc agaucucacg acgugcucac cguccaguuc cugauccuug      120

ggaugcugcu caugacagga ggcguuacgc ggcgcgggag cgccuacuau auguaccugg      180

auagaaacga cgcuggagag gccauuuccu uucccaccac acuuggcaug aauaaaugcu      240

auauccaaau uauggaucug gggcauaugu gugacgcgac caugucuuac gaaugcccua      300

ugcucgauga gggaguggaa ccggacgaug uggacuguug gugcaacaca accagcacau      360

gggucgugua uggcaccugu caccauaaga aaggcgaggc ucggcgcucc agacgggccg      420

uuacgcugcc cucucacagc acccgcaagc ugcagacaag aucccagacc uggcucgaaa      480

gccgggagua cacaaaacau cugauccgcg uggagaauug gauuuucaga aacccugggu      540

uugcccuugc ugccgcugcc aucgcguggc ugcucggauc uuccaccagc cagaagguca      600

ucuaucuggu gaugauucuu cugaucgccc ccgcuuacuc uauucggugc aucggcguuu      660

ccaaucgcga uuucguggaa gggaugagcg gaggcacgug ggucgacauu gugcucgagc      720

acgggggaug ugugacaguc auggcccaag auaagccuac cguugacauc gaacugguga      780

caaccaccgu gucuaacaug gcugagguca gauccuacug cuaugaggcc agcauuuccg      840

auauggcguc ugacagccgg ugucccacac agggcgaagc cuaccuggac aaacaguccg      900

auacgcagua ugugugcaag agaacccucg uugaccgcgg cugggggaau ggauguggcc      960

uguuugggaa aggaagccuu gugacaugcg cuaaguucgc cugcucuaaa aagaugaccg     1020

gcaaguccau ccaaccugag aaccuggaau accggaucau gcucagcguc cacgggucuc     1080

agcauagcgg aaugauugug aaugauacag gccacgagac cgacgaaaac agagcuaaag     1140

uugagaucac gcccaauucc ccgcgcgccg aggccacacu gggcggguuu ggaucucuug     1200

gccuggauug ugaaccucgg accgggcucg acuucagcga ucuguauuac cugaccauga     1260

acaauaagca uuggcucgug cacaaagagu gguuucacga cauuccccug ccuuggcaug     1320

cuggagcgga uacaggcaca ccccacugga acaauaagga agcccuuguc gaguucaaag     1380

acgcucaugc caagagacag acaguggucg ugcugggguc ccaggaaggc gccguucaca     1440

cggcucucgc cggagcgcug gaggcugaga uggauggcgc caaagggcgc cuuagcucug     1500

gacaucugaa gugccggcuc aagauggaca aacugagacu gaagggcgug uccuauagcc     1560

ucuguaccgc cgcuuuuaca uucaccaaaa ucccggccga aacacugcac gggaccguca     1620

cgguggaggu ucaauacgcu ggaaccgacg gcccuugcaa ggugcccgcc cagauggcgg     1680

ucgauaugca gacacuuacc ccuguggggc gccugauuac agccaacccc gugaucaccg     1740

aaucuacgga gaacuccaaa augaugcucg aacuggaucc cccuuuuggc gacagcuaca     1800

ucguuauugg agucggcgag aagaagauca cacaccauug gcaccggucc gggucuacca     1860

uuggaaaggc uuucgaggcc acagugagag gcgcuaaacg cauggccgug cugggggaca     1920

ccgcguggga uuucggaagc gucggcgggg cccuuaauuc ccucggcaaa ggaauccauc     1980

aaauuuuugg cgcugccuuc aagucucugu uugggggaau gagcugguuc ucccagaucc     2040

ugaucggcac ccuccugaug uggcuugggc ugaacaccaa aaauggaagc auuucucuca     2100

ugugucuggc ucugggcggg gugcuuaucu uucucuccac ggccguuagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6687
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6687
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc ccggaagcgc agaucucacg acgugcucac cguccaguuc cugauccuug      120

ggaugcugcu caugacagga gccguuacgc ggcgcggcag cgcuuacuau auguaccugg      180

auagaaacga cgccggggag gcgauuuccu uucccaccac acuuggaaug aauaaaugcu      240

auauccaaau uauggaucug ggccauaugu gugacgcuac caugucuuac gaaugcccua      300

ugcucgauga ggggguggaa ccggacgaug uggacuguug gugcaacaca accagcacau      360

gggucgugua uggaaccugu caccauaaga aaggcgaggc ccggcgcucc agacgggccg      420

uuacgcugcc cucucacagc acccgcaagc ugcagacaag aucccagacc uggcucgaaa      480

gccgggagua cacaaaacau cugauccgcg uggagaauug gauuuucaga aacccuggcu      540

uugcucuugc cgcugccgcg aucgccuggc ugcucggguc uuccaccagc cagaagguca      600

ucuaucuggu gaugauucuu cugaucgcuc ccgccuacuc uauucggugc aucggaguuu      660

ccaaucgcga uuucguggaa ggcaugagcg ggggaacgug ggucgacauu gugcucgagc      720

acggcgggug ugugacaguc auggcucaag auaagccuac cguugacauc gaacugguga      780

caaccaccgu gucuaacaug gccgagguca gauccuacug cuaugaggcg agcauuuccg      840

auauggccuc ugacagccgg ugucccacac agggagaggc uuaccuggac aaacaguccg      900

auacgcagua ugugugcaag agaacccucg uugaccgcgg cuggggcaau ggguguggac      960

uguuuggcaa agggagccuu gugacaugcg ccaaguucgc uugcucuaaa aagaugaccg     1020

gaaaguccau ccaaccugaa aaccuggaau accggaucau gcucagcguc cacggcucuc     1080

agcauagcgg gaugauugug aaugauacag gacacgagac cgacgaaaac agagccaaag     1140

uugagaucac gcccaauucc ccgcgcgccg aggcuacacu gggcggcuuu gggucucuug     1200

gacuggauug ugaaccucgg accggccucg acuucagcga ucuguauuac cugaccauga     1260

acaauaagca uuggcucgug cacaaagagu gguuucacga cauuccccug ccuuggcaug     1320

cgggggccga uacaggaaca ccccacugga acaauaagga agcucuuguc gaguucaaag     1380

acgcccaugc caagagacag acaguggucg ugcugggcuc ccaggaaggg gcuguucaca     1440

cggcccucgc gggcgcucug gaggccgaga uggauggagc caaaggccgc cuuagcucug     1500

ggcaucugaa gugccggcuc aagauggaca aacugagacu gaagggagug uccuauagcc     1560

ucuguaccgc ugccuuuaca uucaccaaaa ucccggcuga aacacugcac ggcaccguca     1620

cgguggaggu ucaauacgcc gggaccgacg gaccuugcaa ggugcccgcg cagauggccg     1680

ucgauaugca gacacuuacc ccugugggcc gccugauuac agcuaacccc gugaucaccg     1740

aaucuacgga gaacuccaaa augaugcucg aacuggaucc cccuuuuggg gacagcuaca     1800

ucguuauugg cgucggagag aagaagauca cacaccauug gcaccggucc ggcucuacca     1860

uugggaaagc cuucgaggcu acagugagag gagccaagcg cauggcggug cugggcgaca     1920

ccgccuggga uuucgggagc gucggaggcg cucuuaauuc ccucgggaaa ggcauccauc     1980

aaauuuuugg agccgcuuuc aagucucugu uuggcgggau gagcugguuc ucccagaucc     2040

ugaucggaac ccuccugaug uggcuuggcc ugaacaccaa aaaugggagc auuucucuca     2100

ugugucuggc ccugggaggc gugcuuaucu uucucuccac ggccguuagc gcugacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6688
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6688
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacauug      720

ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca guugacaucg      780

agcuugugac caccacagug uccaauaugg ccgaagucag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ugaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcgcacgcu aagagacaga cgguggucgu gcucggcucu caggaggggg     1440

ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

ugauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc gcuguuucug     2160

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6689
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6689
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgccagaaa ggagaaaaag cggcgcggcg cugacaccag cguggggauu gucggacugc      120

uccugacaac ggccauggcg gcugaaguua ccagacgggg cuccgccuac uauauguacc      180

uugaucgcaa ugacgccggg gaggcuaucu cuuuccccac aacccuggga augaacaaau      240

gcuauauuca gaucauggau cucggccaca ugugugacgc cacaaugagc uacgaaugcc      300

cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaau accacaucca      360

ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcuagacgg ucucgcagag      420

ccguuacccu ucccagccau uccacacgga agcugcaaac ccgcucucag acauggcucg      480

agagcagaga auacaccaag caccugauuc ggguggagaa cuggaucuuu cgcaauccug      540

gcuucgcgcu ggccgcugcc gcuaucgccu ggcuccuggg guccagcacg ucucagaaag      600

ucauuuaucu ugugaugauc cugcucauug cgcccgccua cuccaucaga ugcauuggag      660

uuagcaaccg ggauuuugug gaaggcaugu cugggggaac augggucgac aucgugcugg      720

agcauggcgg gugugugacc gucauggcuc aggauaagcc uacaguugac auugagcuug      780

ugaccaccac aguguccaau auggccgaag uccgcagcua cugcuaugag gcuucuaucu      840

ccgauauggc cagcgacucc agauguccca cgcaaggaga agccuaccug gacaaacagu      900

cugauaccca guaugugugc aagcggacac ucguugacag aggcuggggc aacgggugug      960

gacuguucgg caaagggagc cuggugaccu gcgcuaaguu ugcgugcucc aagaaaauga     1020

caggaaagag cauccagccu gagaaucucg aauaccgcau uaugcugucu guccacggcu     1080

cccaacacag cgggaugauc gugaacgaua ccggacauga gacggacgag aaucgggcca     1140

aaguugaaau uacaccgaac ucucccagag cugaggccac ccuuggcggc uucgggagcc     1200

ugggacucga uugugaaccu cgcaccggcc uggacuuuuc cgaucuuuau uaccugacaa     1260

ugaauaacaa gcacuggcuc gugcauaaag agugguucca cgacaucccc cugccuuggc     1320

acgccggggc ugauaccgga acaccccauu ggaauaacaa ggaagcccug gucgaguuua     1380

aagacgcgca cgcuaagcgg cagacggugg ucgugcucgg cucucaggag ggggccguuc     1440

auaccgcccu ggcuggcgcc cuugaagcug agauggaugg agccaagggc agacugagcu     1500

ccgggcaccu caaaugccgc cugaagaugg acaaacuucg gcugaaggga gugagcuauu     1560

cucucuguac agcggccuuc accuuuacaa aaauuccggc ugaaacccug cauggcacgg     1620

ucaccgugga gguucaguac gccgggacag acggaccuug caaggugccc gcucaaaugg     1680

ccgucgauau gcagacccug acaccugugg gcagacucau caccgcgaac cccgugauca     1740

cggaauccac agagaauagc aagaugaugc uggagcuuga ucccccuuuc ggggacucuu     1800

acauuguuau cggcgucgga gagaaaaaga uuacccacca cuggcaucgc uccggcagca     1860

caaucgggaa agccuuugaa gcuaccgugc ggggagccaa gagaauggcu gugcugggcg     1920

acaccgccug ggauuucggg uccgucggag gcgcccucaa cucucugggg aaaggcauuc     1980

accagaucuu cggagcugcg uuuaagagcc uguucggcgg gauguccugg uuuucucaaa     2040

uccuuauugg aacacuccug auguggcugg gccucaauac gaagaacggg agcaucuccc     2100

ugaugugucu ugcccuggga ggcgugcuca uuuuccugag caccgcuguu ucugccugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6690
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6690
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uuaacgccag aaaggagaaa aagcggcgcg gcgcugacac cagcgugggg aucgucggac      120

ugcuccugac aacggccaug gcggcugaag uuaccagacg gggcuccgcc uacuauaugu      180

accuugaucg caaugacgcc ggggaggcua uuucuuuccc cacaacccug ggaaugaaca      240

aaugcuauau ccagauuaug gaucucggcc acauguguga cgccacaaug agcuacgaau      300

gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc aauaccacau      360

ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggcuaga cggucucgca      420

gagccguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu cagacauggc      480

ucgagagcag agaauacacc aagcaccuga uccgggugga gaacuggauc uuucgcaauc      540

cuggcuucgc gcuggccgcu gccgcuauug ccuggcuccu gggguccagc acgucucaga      600

aagucaucua ucuugugaug auucugcuca ucgcgcccgc cuacuccauu agaugcaucg      660

gaguuagcaa ccgggauuuu guggaaggca ugucuggggg aacauggguc gacauugugc      720

uggagcaugg cgggugugug accgucaugg cucaggauaa gccuacaguu gacaucgagc      780

uugugaccac cacagugucc aauauggccg aaguccgcag cuacugcuau gaggcuucua      840

ucuccgauau ggccagcgac uccagauguc ccacgcaagg agaagccuac cuggacaaac      900

agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg ggcaacgggu      960

guggacuguu cggcaaaggg agccugguga ccugcgcuaa guuugcgugc uccaagaaaa     1020

ugacaggaaa gagcauucag ccugagaauc ucgaauaccg caucaugcug ucuguccacg     1080

gcucccaaca cagcgggaug auugugaacg auaccggaca ugagacggac gagaaucggg     1140

ccaaaguuga aaucacaccg aacucuccca gagcugaggc cacccuuggc ggcuucggga     1200

gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu uauuaccuga     1260

caaugaauaa caagcacugg cucgugcaua aagagugguu ccacgacauu ccccugccuu     1320

ggcacgccgg ggcugauacc ggaacacccc auuggaauaa caaggaagcc cuggucgagu     1380

uuaaagacgc gcacgcuaag cggcagacgg uggucgugcu cggcucucag gagggggccg     1440

uucauaccgc ccuggcuggc gcccuugaag cugagaugga uggagccaag ggcagacuga     1500

gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag ggagugagcu     1560

auucucucug uacagcggcc uucaccuuua caaaaauccc ggcugaaacc cugcauggca     1620

cggucaccgu ggagguucag uacgccggga cagacggacc uugcaaggug cccgcucaaa     1680

uggccgucga uaugcagacc cugacaccug ugggcagacu caucaccgcg aaccccguga     1740

uuacggaauc cacagagaau agcaagauga ugcuggagcu ugaucccccu uucggggacu     1800

cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau cgcuccggca     1860

gcacaauugg gaaagccuuu gaagcuaccg ugcggggagc caagagaaug gcugugcugg     1920

gcgacaccgc cugggauuuc ggguccgucg gaggcgcccu caacucucug gggaaaggca     1980

uccaccagau cuucggagcu gcguuuaaga gccuguucgg cgggaugucc ugguuuucuc     2040

aaauucuuau cggaacacuc cugauguggc ugggccucaa uacgaagaac gggagcauuu     2100

cccugaugug ucuugcccug ggaggcgugc ucaucuuccu gagcaccgcu guuucugccu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6691
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6691
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaagga gaaaaagcgg cgcggcgcug acaccagcgu ggggaucguc ggacugcucc      120

ugacaacggc cauggcggcu gaaguuacca gacggggcuc cgccuacuau auguaccuug      180

aucgcaauga cgccggggag gcuauuucuu uccccacaac ccugggaaug aacaaaugcu      240

auauccagau uauggaucuc ggccacaugu gugacgccac aaugagcuac gaaugcccua      300

ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaauacc acauccaccu      360

gggucgugua uggaacgugu caucacaaga aaggcgaggc uagacggucu cgcagagccg      420

uuacccuucc cagccauucc acacggaagc ugcaaacccg cucucagaca uggcucgaga      480

gcagagaaua caccaagcac cugauccggg uggagaacug gauuuuucgc aauccuggcu      540

ucgcgcuggc cgcugccgcu aucgccuggc uccugggguc cagcacgucu cagaaaguca      600

ucuaucuugu gaugauucug cucaucgcgc ccgccuacuc cauuagaugc aucggaguua      660

gcaaccggga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu gugcuggagc      720

auggcgggug ugugaccguc auggcucagg auaagccuac aguugacauc gagcuuguga      780

ccaccacagu guccaauaug gccgaagucc gcagcuacug cuaugaggcu ucuauuuccg      840

auauggccag cgacuccaga ugucccacgc aaggagaagc cuaccuggac aaacagucug      900

auacccagua ugugugcaag cggacacucg uugacagagg cuggggcaac ggguguggac      960

uguucggcaa agggagccug gugaccugcg cuaaguuugc gugcuccaag aaaaugacag     1020

gaaagagcau ccagccugag aaucucgaau accgcaucau gcugucuguc cacggcuccc     1080

aacacagcgg gaugauugug aacgauaccg gacaugagac ggacgagaau cgggccaaag     1140

uugaaaucac accgaacucu cccagagcug aggccacccu uggcggcuuc gggagccugg     1200

gacucgauug ugaaccucgc accggccugg acuuuuccga ucuuuauuac cugacaauga     1260

auaacaagca cuggcucgug cauaaagagu gguuccacga cauuccccug ccuuggcacg     1320

ccggggcuga uaccggaaca ccccauugga auaacaagga agcccugguc gaguuuaaag     1380

acgcgcacgc uaagcggcag acgguggucg ugcucggcuc ucaggagggg gccguucaua     1440

ccgcccuggc uggcgcccuu gaagcugaga uggauggagc caagggcaga cugagcuccg     1500

ggcaccucaa augccgccug aagauggaca aacuucggcu gaagggagug agcuauucuc     1560

ucuguacagc ggccuucacc uuuacaaaaa ucccggcuga aacccugcau ggcacgguca     1620

ccguggaggu ucaguacgcc gggacagacg gaccuugcaa ggugcccgcu caaauggccg     1680

ucgauaugca gacccugaca ccugugggca gacucauuac cgcgaacccc gugaucacgg     1740

aauccacaga gaauagcaag augaugcugg agcuugaucc cccuuucggg gacucuuaca     1800

ucguuauugg cgucggagag aaaaagauca cccaccacug gcaucgcucc ggcagcacaa     1860

uugggaaagc cuuugaagcu accgugcggg gagccaagag aauggcugug cugggcgaca     1920

ccgccuggga uuucgggucc gucggaggcg cccucaacuc ucuggggaaa ggcauccacc     1980

agauuuucgg agcugcguuu aagagccugu ucggcgggau guccugguuu ucucaaaucc     2040

uuaucggaac acuccugaug uggcugggcc ucaauacgaa gaacgggagc auuucccuga     2100

ugugucuugc ccugggaggc gugcucaucu uccugagcac cgcuguuucu gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6692
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6692
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaggagaa aaagcggcgc ggcgcugaca ccagcguggg gaucgucgga cugcuccuga      120

caacggccau ggcggcugaa guuaccagac ggggcuccgc cuacuauaug uaccuugauc      180

gcaacgacgc cggggaggcu auuucuuucc ccacaacccu gggaaugaau aaaugcuaua      240

uccagauuau ggaucucggc cacaugugug acgccacaau gagcuacgaa ugcccuaugc      300

uggaugaggg gguggaaccg gacgaugugg acuguuggug caacaccaca uccaccuggg      360

ucguguaugg aacgugucau cacaagaaag gcgaggcuag acggucucgc agagccguua      420

cccuucccag ccauuccaca cggaagcugc aaacccgcuc ucagacaugg cucgagagca      480

gagaauacac caagcaccug auccgggugg agaauuggau uuuucgcaac ccuggcuucg      540

cgcuggccgc ugccgcuauc gccuggcucc ugggguccag cacgucucag aaagucaucu      600

aucuugugau gauucugcuc aucgcgcccg ccuacuccau uagaugcauc ggaguuagca      660

aucgggauuu uguggaaggc augucugggg gaacaugggu cgacauugug cuggagcaug      720

gcgggugugu gaccgucaug gcucaggaua agccuacagu ugacaucgag cuugugacca      780

ccacaguguc caacauggcc gaaguccgca gcuacugcua ugaggcuucu auuuccgaua      840

uggccagcga cuccagaugu cccacgcaag gagaagccua ccuggacaaa cagucugaua      900

cccaguaugu gugcaagcgg acacucguug acagaggcug gggcaauggg uguggacugu      960

ucggcaaagg gagccuggug accugcgcua aguuugcgug cuccaagaaa augacaggaa     1020

agagcaucca gccugagaac cucgaauacc gcaucaugcu gucuguccac ggcucccaac     1080

acagcgggau gauugugaau gauaccggac augagacgga cgagaaccgg gccaaaguug     1140

aaaucacacc caauucuccc agagcugagg ccacccuugg cggcuucggg agccugggac     1200

ucgauuguga accucgcacc ggccuggacu uuuccgaucu uuauuaccug acaaugaaca     1260

auaagcacug gcucgugcau aaagaguggu uccacgacau uccccugccu uggcacgccg     1320

gggcugauac cggaacaccc cauuggaaca auaaggaagc ccuggucgag uuuaaagacg     1380

cgcacgcuaa gcggcagacg guggucgugc ucggcucuca ggagggggcc guucauaccg     1440

cccuggcugg cgcccuugaa gcugagaugg auggagccaa gggcagacug agcuccgggc     1500

accucaaaug ccgccugaag auggacaaac uucggcugaa gggagugagc uauucucucu     1560

guacagcggc cuucaccuuu acaaaaaucc cggcugaaac ccugcauggc acggucaccg     1620

uggagguuca guacgccggg acagacggac cuugcaaggu gcccgcucaa auggccgucg     1680

auaugcagac ccugacaccu gugggcagac ucauuaccgc gaaccccgug aucacggaau     1740

ccacagagaa cagcaagaug augcuggagc uugacccgcc uuucggggau ucuuacaucg     1800

uuauuggcgu cggagagaaa aagaucaccc accacuggca ucgcuccggc agcacaauug     1860

ggaaagccuu ugaagcuacc gugcggggag ccaagagaau ggcugugcug ggcgacaccg     1920

ccugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaaaggc auccaccaga     1980

uuuucggagc ugcguuuaag agccuguucg gcgggauguc cugguuuucu caaauccuua     2040

ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu ucccugaugu     2100

gucuugcccu gggaggcgug cucaucuucc ugagcaccgc uguuucugcc ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6693
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6693
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aggagaaaaa gcggcgcggc gccgacacca gcguggggau cgucggacug cuccugacaa      120

cggcuauggc cgcggaaguu accagacggg gcuccgcuua cuauauguac cuugaucgca      180

acgacgccgg ggaggccauu ucuuucccca caacccuggg aaugaauaaa ugcuauaucc      240

agauuaugga ucucggccac augugugacg cuacaaugag cuacgaaugc ccuaugcugg      300

augagggggu ggaaccggac gauguggacu guuggugcaa caccacaucc accugggucg      360

uguauggaac gugucaucac aagaaaggcg aggccagacg gucucgcaga gcuguuaccc      420

uucccagcca uuccacacgg aagcugcaaa cccgcucuca gacauggcuc gagagcagag      480

aauacaccaa gcaccugauc cggguggaga auuggauuuu ucgcaacccu ggcuucgccc      540

uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaaa gucaucuauc      600

uugugaugau ucugcucauc gcccccgcgu acuccauuag augcaucgga guuagcaauc      660

gggauuuugu ggaaggcaug ucugggggaa caugggucga cauugugcug gagcauggcg      720

ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu gugaccacca      780

caguguccaa cauggcugaa guccgcagcu acugcuauga ggccucuauu uccgauaugg      840

cuagcgacuc cagauguccc acgcaaggag aagccuaccu ggacaaacag ucugauaccc      900

aguaugugug caagcggaca cucguugaca gaggcugggg caaugggugu ggacuguucg      960

gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caagaaaaug acaggaaaga     1020

gcauccagcc ugagaaccuc gaauaccgca ucaugcuguc uguccacggc ucccaacaca     1080

gcgggaugau ugugaaugau accggacaug agacggacga gaaccgggcg aaaguugaaa     1140

ucacacccaa uucucccaga gccgaggcua cccuuggcgg cuucgggagc cugggacucg     1200

auugugaacc ucgcaccggc cuggacuuuu ccgaucuuua uuaccugaca augaacaaua     1260

agcacuggcu cgugcauaaa gagugguucc acgacauucc ccugccuugg cacgccgggg     1320

ccgauaccgg aacaccccau uggaacaaua aggaagcucu ggucgaguuu aaagacgccc     1380

acgcgaagcg gcagacggug gucgugcucg gcucucagga gggggcuguu cauaccgccc     1440

uggccggcgc ucuugaagcc gagauggaug gagcuaaggg cagacugagc uccgggcacc     1500

ucaaaugccg ccugaagaug gacaaacuuc ggcugaaggg agugagcuau ucucucugua     1560

cagccgcguu caccuuuaca aaaaucccgg ccgaaacccu gcauggcacg gucaccgugg     1620

agguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug gcugucgaua     1680

ugcagacccu gacaccugug ggcagacuca uuaccgccaa ccccgugauc acggaaucca     1740

cagagaacag caagaugaug cuggagcuug acccgccuuu cggggauucu uacaucguua     1800

uuggcgucgg agagaaaaag aucacccacc acuggcaucg cuccggcagc acaauuggga     1860

aagcguuuga agccaccgug cggggagcua agagaauggc cgugcugggc gacaccgcuu     1920

gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc caccagauuu     1980

ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa auccuuaucg     2040

gaacacuccu gauguggcug ggccucaaca cgaagaaugg gagcauuucc cugauguguc     2100

uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcuuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6694
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6694
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

agaaaaagag acggggcgcc gacaccagcg uggggaucgu cggacugcuc cugacaacgg      120

cuauggccgc ggaaguuacc cgcagaggcu ccgcuuacua uauguaccuu gaucggaacg      180

acgccgggga ggccauuucu uuccccacaa cccugggaau gaauaaaugc uauauccaga      240

uuauggaucu cggccacaug ugugacgcua caaugagcua cgaaugcccu augcuggaug      300

agggggugga accggacgau guggacuguu ggugcaacac cacauccacc ugggucgugu      360

auggaacgug ucaucacaag aaaggcgagg cccgcagauc ucggcgcgcu guuacccuuc      420

ccagccauuc cacaagaaag cugcaaaccc ggucucagac auggcucgag agccgcgaau      480

acaccaagca ccugaucaga guggagaauu ggauuuuucg gaacccuggc uucgcccugg      540

cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc aucuaucuug      600

ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu agcaauagag      660

auuuugugga aggcaugucu gggggaacau gggucgacau ugugcuggag cauggcgggu      720

gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug accaccacag      780

uguccaacau ggcugaaguc cggagcuacu gcuaugaggc cucuauuucc gauauggcua      840

gcgacucccg cugucccacg caaggagaag ccuaccugga caaacagucu gauacccagu      900

augugugcaa gagaacacuc guugaccggg gcuggggcaa ugggugugga cuguucggca      960

aagggagccu ggugaccugc gccaaguuug cuugcuccaa gaaaaugaca ggaaagagca     1020

uccagccuga gaaccucgaa uacagaauca ugcugucugu ccacggcucc caacacagcg     1080

ggaugauugu gaaugauacc ggacaugaga cggacgagaa ccgcgcgaaa guugaaauca     1140

cacccaauuc uccccgggcc gaggcuaccc uuggcggcuu cgggagccug ggacucgauu     1200

gugaaccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug aacaauaagc     1260

acuggcucgu gcauaaagag ugguuccacg acauuccccu gccuuggcac gccggggccg     1320

auaccggaac accccauugg aacaauaagg aagcucuggu cgaguuuaaa gacgcccacg     1380

cgaagcgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau accgcccugg     1440

ccggcgcucu ugaagccgag auggauggag cuaagggccg gcugagcucc gggcaccuca     1500

aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu cucuguacag     1560

ccgcguucac cuuuacaaaa aucccggccg aaacccugca uggcacgguc accguggagg     1620

uucaguacgc ugggacagac ggaccuugca aggugcccgc ccaaauggcu gucgauaugc     1680

agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg gaauccacag     1740

agaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac aucguuauug     1800

gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca auugggaaag     1860

cguuugaagc caccgugcgc ggagcuaagc ggauggccgu gcugggcgac accgcuuggg     1920

auuucggguc cgucggaggc gcccucaauu cucuggggaa aggcauccac cagauuuucg     1980

gagccgcuuu uaagagccug uucggcggga uguccugguu uucucaaauc cuuaucggaa     2040

cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug augugucuug     2100

cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcuugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6695
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6695
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagacg gggcgccgac accagcgugg ggaucgucgg acugcuccug acaacggcua      120

uggccgcgga aguuacccgc agaggcuccg cuuacuauau guaccuugau cggaacgacg      180

ccggggaggc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau auccagauua      240

uggaucucgg ccacaugugu gacgcuacaa ugagcuacga augcccuaug cuggaugagg      300

ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg gucguguaug      360

gaacguguca ucacaaaaag ggcgaggccc gcagaucucg gcgcgcuguu acccuuccca      420

gccauuccac aagaaaacug caaacccggu cucagacaug gcucgagagc cgcgaauaca      480

ccaagcaccu gaucagagug gagaauugga uuuuucggaa cccuggcuuc gcccuggcgg      540

ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaggucauc uaucuuguga      600

ugauucugcu caucgccccc gcguacucca uucgcugcau cggaguuagc aauagagauu      660

uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau ggcgggugug      720

ugaccgucau ggcccaggau aaaccuacag uugacaucga gcuugugacc accacagugu      780

ccaacauggc ugaaguccgg agcuacugcu augaggccuc uauuuccgau auggcuagcg      840

acucccgcug ucccacgcaa ggagaagccu accuggacaa gcagucugau acccaguaug      900

ugugcaaaag aacacucguu gaccggggcu ggggcaaugg guguggacug uucggcaagg      960

ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa gaugacagga aaaagcaucc     1020

agccugagaa ccucgaauac agaaucaugc ugucugucca cggcucccaa cacagcggga     1080

ugauugugaa ugauaccgga caugagacgg acgagaaccg cgcgaagguu gaaaucacac     1140

ccaauucucc ccgggccgag gcuacccuug gcggcuucgg gagccuggga cucgauugug     1200

aaccuagaac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac aauaaacacu     1260

ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc ggggccgaua     1320

ccggaacacc ccauuggaac aauaaagaag cucuggucga guuuaaggac gcccacgcga     1380

aacgccagac gguggucgug cucggcucuc aggagggggc uguucauacc gcccuggccg     1440

gcgcucuuga agccgagaug gauggagcua agggccggcu gagcuccggg caccucaagu     1500

gcagacugaa aauggacaaa cuucgccuga agggagugag cuauucucuc uguacagccg     1560

cguucaccuu uacaaagauc ccggccgaaa cccugcaugg cacggucacc guggagguuc     1620

aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc gauaugcaga     1680

cccugacacc ugugggccgg cucauuaccg ccaaccccgu gaucacggaa uccacagaga     1740

acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc guuauuggcg     1800

ucggagaaaa gaaaaucacc caccacuggc auagauccgg cagcacaauu gggaaggcgu     1860

uugaggccac cgugcgcgga gcuaaacgga uggccgugcu gggcgacacc gcuugggauu     1920

ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag auuuucggag     1980

ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu aucggaacac     2040

uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug ugucuugcgc     2100

ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc uugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6696
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6696
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagacgggg cgccgacacc agcgugggga ucgucggacu gcuccugaca acggcuaugg      120

ccgcggaggu uacccgcaga ggcuccgcuu acuauaugua ccuugaucgg aacgacgccg      180

gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc cagauuaugg      240

aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug gaugaagggg      300

uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc guguauggaa      360

cgugucauca caaaaagggc gaagcccgca gaucucggcg cgcuguuacc cuucccagcc      420

auuccacaag aaaacugcaa acccggucuc agacauggcu cgagagccgc gaguacacca      480

agcaccugau cagaguggaa aauuggauuu uucggaaccc uggcuucgcc cuggcggccg      540

cugccaucgc uuggcuccug ggguccagca cgucucagaa ggucaucuau cuugugauga      600

uucugcucau cgcccccgcg uacuccauuc gcugcaucgg aguuagcaau agagauuuug      660

uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc ggguguguga      720

ccgucauggc ccaggauaaa ccuacaguug acaucgagcu ugugaccacc acagugucca      780

acauggcuga gguccggagc uacugcuaug aagccucuau uuccgauaug gcuagcgacu      840

cccgcugucc cacgcaagga gaggccuacc uggacaagca gucugauacc caguaugugu      900

gcaaaagaac acucguugac cggggcuggg gcaaugggug uggacuguuc ggcaagggga      960

gccuggugac cugcgccaaa uuugcuugcu ccaagaagau gacaggaaaa agcauccagc     1020

cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac agcgggauga     1080

uugugaauga uaccggacau gaaacggacg agaaccgcgc gaagguugag aucacaccca     1140

auucuccccg ggccgaagcu acccuuggcg gcuucgggag ccugggacuc gauugugagc     1200

cuagaaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau aaacacuggc     1260

ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg gccgauaccg     1320

gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc cacgcgaaac     1380

gccagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc cuggccggcg     1440

cucuugaggc cgaaauggau ggagcuaagg gccggcugag cuccgggcac cucaagugca     1500

gacugaaaau ggacaaacuu cgccugaagg gagugagcua uucucucugu acagccgcgu     1560

ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug gaaguucagu     1620

acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau augcagaccc     1680

ugacaccugu gggccggcuc auuaccgcca accccgugau cacggagucc acagaaaaca     1740

gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu auuggcgucg     1800

gagagaagaa aaucacccac cacuggcaua gauccggcag cacaauuggg aaggcguuug     1860

aagccaccgu gcgcggagcu aaacggaugg ccgugcuggg cgacaccgcu ugggauuucg     1920

gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu uucggagccg     1980

cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc ggaacacucc     2040

ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu cuugcgcugg     2100

gaggcgugcu caucuuccug agcaccgccg uuucugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6697
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6697
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

gacggggcgc cgacaccagc guggggaucg ucggacugcu ccugacaacg gcuauggccg      120

cggagguuac ccgcagaggc uccgcuuacu auauguaccu ugaucggaac gacgccgggg      180

aagccauuuc uuuccccaca acccugggaa ugaauaaaug cuauauccag auuauggauc      240

ucggccacau gugugacgcu acaaugagcu acgagugccc uaugcuggau gaaggggugg      300

agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug uauggaacgu      360

gucaucacaa gaaaggcgaa gcccgcagau cucggcgcgc uguuacccuu cccagccauu      420

ccacaagaaa gcugcaaacc cggucucaga cauggcucga gagccgcgag uacaccaaac      480

accugaucag aguggaaaau uggauuuuuc ggaacccugg cuucgcccug gcggccgcug      540

ccaucgcuug gcuccugggg uccagcacgu cucagaaggu caucuaucuu gugaugauuc      600

ugcucaucgc ccccgcguac uccauucgcu gcaucggagu uagcaauaga gauuuugugg      660

agggcauguc ugggggaaca ugggucgaca uugugcugga acauggcggg ugugugaccg      720

ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca guguccaaca      780

uggcugaggu ccggagcuac ugcuaugaag ccucuauuuc cgauauggcu agcgacuccc      840

gcugucccac gcaaggagag gccuaccugg acaaacaguc ugauacccag uaugugugca      900

agagaacacu cguugaccgg ggcuggggca augggugugg acuguucggc aaagggagcc      960

uggugaccug cgccaaguuu gcuugcucca aaaagaugac aggaaagagc auccagccug     1020

aaaaccucga guacagaauc augcugucug uccacggcuc ccaacacagc gggaugauug     1080

ugaaugauac cggacaugaa acggacgaga accgcgcgaa aguugagauc acacccaauu     1140

cuccccgggc cgaagcuacc cuuggcggcu ucgggagccu gggacucgau ugugagccua     1200

gaaccggccu ggacuuuucc gaucuuuauu accugacaau gaacaauaag cacuggcucg     1260

ugcauaaaga augguuccac gacauucccc ugccuuggca cgccggggcc gauaccggaa     1320

caccccauug gaacaauaag gaggcucugg ucgaauuuaa agacgcccac gcgaagcgcc     1380

agacgguggu cgugcucggc ucucaggagg gggcuguuca uaccgcccug gccggcgcuc     1440

uugaggccga aauggaugga gcuaaaggcc ggcugagcuc cgggcaccuc aagugcagac     1500

ugaagaugga caaacuucgc cugaagggag ugagcuauuc ucucuguaca gccgcguuca     1560

ccuuuacaaa aaucccggcc gagacccugc auggcacggu caccguggaa guucaguacg     1620

cugggacaga cggaccuugc aaggugcccg cccaaauggc ugucgauaug cagacccuga     1680

caccuguggg ccggcucauu accgccaacc ccgugaucac ggaguccaca gaaaacagca     1740

aaaugaugcu ggagcuugac ccgccuuucg gggauucuua caucguuauu ggcgucggag     1800

agaagaagau cacccaccac uggcauagau ccggcagcac aauugggaaa gcguuugaag     1860

ccaccgugcg cggagcuaag cggauggccg ugcugggcga caccgcuugg gauuucgggu     1920

ccgucggagg cgcccucaau ucucugggga aaggcaucca ccagauuuuc ggagccgcuu     1980

uuaagagccu guucggcggg auguccuggu uuucucaaau ccuuaucgga acacuccuga     2040

uguggcuggg ccucaacacg aaaaauggga gcauuucccu gaugugucuu gcgcugggag     2100

gcgugcucau cuuccugagc accgccguuu cugcuugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6698
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6698
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgccga caccagcgug gggaucgucg gacugcuccu gacaacggcu auggccgcgg      120

agguuacccg cagaggcucc gcuuacuaua uguaccuuga ucggaacgac gccggggaag      180

ccauuucuuu ccccacaacc cugggaauga auaagugcua uauccagauu auggaucucg      240

gccacaugug ugacgcuaca augagcuacg agugcccuau gcuggaugaa gggguggagc      300

cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau ggaacguguc      360

aucacaaaaa gggcgaagcc cgcagaucuc ggcgcgcugu uacccuuccc agccauucca      420

caagaaaacu gcaaacccgg ucucagacau ggcucgagag ccgcgaguac accaagcacc      480

ugaucagagu ggaaaauugg auuuuucgga acccuggcuu cgcccuggcg gccgcugcca      540

ucgcuuggcu ccuggggucc agcacgucuc agaaagucau cuaucuugug augauucugc      600

ucaucgcccc cgcguacucc auucgcugca ucggaguuag caauagagau uuuguggagg      660

gcaugucugg gggaacaugg gucgacauug ugcuggaaca uggcgggugu gugaccguca      720

uggcccagga uaagccuaca guugacaucg agcuugugac caccacagug uccaacaugg      780

cugagguccg gagcuacugc uaugaagccu cuauuuccga uauggcuagc gacucccgcu      840

gucccacgca aggagaggcc uaccuggaca agcagucuga uacccaguau gugugcaaaa      900

gaacacucgu ugaccggggc uggggcaaug gguguggacu guucggcaag gggagccugg      960

ugaccugcgc caaauuugcu ugcuccaaga aaaugacagg aaagagcauc cagccugaaa     1020

accucgagua cagaaucaug cugucugucc acggcuccca acacagcggg augauuguga     1080

augauaccgg acaugaaacg gacgagaacc gcgcgaaggu ugagaucaca cccaauucuc     1140

cccgggccga agcuacccuu ggcggcuucg ggagccuggg acucgauugu gagccuagaa     1200

ccggccugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac uggcucgugc     1260

auaaggaaug guuccacgac auuccccugc cuuggcacgc cggggccgau accggaacac     1320

cccauuggaa caauaaagag gcucuggucg aauuuaagga cgcccacgcg aaacgccaga     1380

cgguggucgu gcucggcucu caggaggggg cuguucauac cgcccuggcc ggcgcucuug     1440

aggccgaaau ggauggagcu aagggccggc ugagcuccgg gcaccucaaa ugcagacuga     1500

agauggacaa acuucgccug aagggaguga gcuauucucu cuguacagcc gcguucaccu     1560

uuacaaagau cccggccgag acccugcaug gcacggucac cguggaaguu caguacgcug     1620

ggacagacgg accuugcaaa gugcccgccc aaauggcugu cgauaugcag acccugacac     1680

cugugggccg gcucauuacc gccaaccccg ugaucacgga guccacagaa aacagcaaga     1740

ugaugcugga gcuugacccg ccuuucgggg auucuuacau cguuauuggc gucggagaga     1800

aaaagaucac ccaccacugg cauagauccg gcagcacaau ugggaaggcg uuugaagcca     1860

ccgugcgcgg agcuaaacgg auggccgugc ugggcgacac cgcuugggau uucggguccg     1920

ucggaggcgc ccucaauucu cuggggaagg gcauccacca gauuuucgga gccgcuuuua     1980

aaagccuguu cggcgggaug uccugguuuu cucaaauccu uaucggaaca cuccugaugu     2040

ggcugggccu caacacgaag aaugggagca uuucccugau gugucuugcg cugggaggcg     2100

ugcucaucuu ccugagcacc gccguuucug cuugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6699
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6699
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug gccgcggagg      120

uuacccggcg cggcuccgcu uacuauaugu accuugauag aaacgacgcc ggggaagcca      180

uuucuuuccc cacaacccug ggaaugaaua agugcuauau ccagauuaug gaucucggcc      240

acauguguga cgcuacaaug agcuacgagu gcccuaugcu ggaugaaggg guggagccgg      300

acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga acgugucauc      360

acaaaaaggg cgaagcccgg cgcucuagac gggcuguuac ccuucccagc cauuccacac      420

gcaaacugca aaccagaucu cagacauggc ucgagagccg ggaguacacc aagcaccuga      480

uccgcgugga aaauuggauu uuuagaaacc cuggcuucgc ccuggcggcc gcugccaucg      540

cuuggcuccu gggguccagc acgucucaga aagucaucua ucuugugaug auucugcuca      600

ucgcccccgc guacuccauu cggugcaucg gaguuagcaa ucgcgauuuu guggagggca      660

ugucuggggg aacauggguc gacauugugc uggaacaugg cgggugugug accgucaugg      720

cccaggauaa gccuacaguu gacaucgagc uugugaccac cacagugucc aacauggcug      780

aggucagaag cuacugcuau gaagccucua uuuccgauau ggcuagcgac ucccgguguc      840

ccacgcaagg agaggccuac cuggacaagc agucugauac ccaguaugug ugcaaacgca      900

cacucguuga cagaggcugg ggcaaugggu guggacuguu cggcaagggg agccugguga      960

ccugcgccaa auuugcuugc uccaagaaaa ugacaggaaa gagcauccag ccugaaaacc     1020

ucgaguaccg gaucaugcug ucuguccacg gcucccaaca cagcgggaug auugugaaug     1080

auaccggaca ugaaacggac gagaacagag cgaagguuga gaucacaccc aauucucccc     1140

gcgccgaagc uacccuuggc ggcuucggga gccugggacu cgauugugag ccucggaccg     1200

gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaaacacugg cucgugcaua     1260

aggaaugguu ccacgacauu ccccugccuu ggcacgccgg ggccgauacc ggaacacccc     1320

auuggaacaa uaaagaggcu cuggucgaau uuaaggacgc ccacgcgaaa agacagacgg     1380

uggucgugcu cggcucucag gagggggcug uucauaccgc ccuggccggc gcucuugagg     1440

ccgaaaugga uggagcuaag ggccgccuga gcuccgggca ccucaaaugc cggcugaaga     1500

uggacaaacu uagacugaag ggagugagcu auucucucug uacagccgcg uucaccuuua     1560

caaagauccc ggccgagacc cugcauggca cggucaccgu ggaaguucag uacgcuggga     1620

cagacggacc uugcaaagug cccgcccaaa uggcugucga uaugcagacc cugacaccug     1680

ugggccgccu cauuaccgcc aaccccguga ucacggaguc cacagaaaac agcaagauga     1740

ugcuggagcu ugacccgccu uucggggauu cuuacaucgu uauuggcguc ggagagaaaa     1800

agaucaccca ccacuggcau cgguccggca gcacaauugg gaaggcguuu gaagccaccg     1860

ugagaggagc uaaacgcaug gccgugcugg gcgacaccgc uugggauuuc ggguccgucg     1920

gaggcgcccu caauucucug gggaagggca uccaccagau uuucggagcc gcuuuuaaaa     1980

gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc cugauguggc     2040

ugggccucaa cacgaagaau gggagcauuu cccugaugug ucuugcgcug ggaggcgugc     2100

ucaucuuccu gagcaccgcc guuucugcuu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6700
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6700
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag cguggggauc gucggacugc uccugacaac ggcuauggcc gcggagguua      120

ccagacgggg cuccgcuuac uauauguacc uugaucgcaa cgacgccggg gaagccauuu      180

cuuuccccac aacccuggga augaauaagu gcuauaucca gauuauggau cucggccaca      240

ugugugacgc uacaaugagc uacgagugcc cuaugcugga ugaaggggug gagccggacg      300

auguggacug uuggugcaac accacaucca ccugggucgu guauggaacg ugucaucaca      360

aaaagggcga agccagacgg ucucgcagag cuguuacccu ucccagccau uccacacgga      420

aacugcaaac ccgcucucag acauggcucg agagcagaga guacaccaag caccugaucc      480

ggguggaaaa uuggauuuuu cgcaacccug gcuucgcccu ggcggccgcu gccaucgcuu      540

ggcuccuggg guccagcacg ucucagaaag ucaucuaucu ugugaugauu cugcucaucg      600

cccccgcgua cuccauuaga ugcaucggag uuagcaaucg ggauuuugug gagggcaugu      660

cugggggaac augggucgac auugugcugg aacauggcgg gugugugacc gucauggccc      720

aggauaagcc uacaguugac aucgagcuug ugaccaccac aguguccaac auggcugagg      780

uccgcagcua cugcuaugaa gccucuauuu ccgauauggc uagcgacucc agauguccca      840

cgcaaggaga ggccuaccug gacaagcagu cugauaccca guaugugugc aaacggacac      900

ucguugaccg cggcuggggc aaugggugug gacuguucgg caaggggagc cuggugaccu      960

gcgccaaauu ugcuugcucc aagaaaauga caggaaagag cauccagccu gaaaaccucg     1020

aguacagaau caugcugucu guccacggcu cccaacacag cgggaugauu gugaaugaua     1080

ccggacauga aacggacgag aaccgggcga agguugagau cacacccaau ucucccagag     1140

ccgaagcuac ccuuggcggc uucgggagcc ugggacucga uugugagccu cgcaccggcc     1200

uggacuuuuc cgaucuuuau uaccugacaa ugaacaauaa acacuggcuc gugcauaagg     1260

aaugguucca cgacauuccc cugccuuggc acgccggggc cgauaccgga acaccccauu     1320

ggaacaauaa agaggcucug gucgaauuua aggacgccca cgcgaaacgg cagacggugg     1380

ucgugcucgg cucucaggag ggggcuguuc auaccgcccu ggccggcgcu cuugaggccg     1440

aaauggaugg agcuaagggc agacugagcu ccgggcaccu caaaugccgc cugaagaugg     1500

acaaacuucg gcugaaggga gugagcuauu cucucuguac agccgcguuc accuuuacaa     1560

agaucccggc cgagacccug cauggcacgg ucaccgugga aguucaguac gcugggacag     1620

acggaccuug caaagugccc gcccaaaugg cugucgauau gcagacccug acaccugugg     1680

gcagacucau uaccgccaac cccgugauca cggaguccac agaaaacagc aagaugaugc     1740

uggagcuuga cccgccuuuc ggggauucuu acaucguuau uggcgucgga gagaaaaaga     1800

ucacccacca cuggcaucgc uccggcagca caauugggaa ggcguuugaa gccaccgugc     1860

ggggagcuaa aagaauggcc gugcugggcg acaccgcuug ggauuucggg uccgucggag     1920

gcgcccucaa uucucugggg aagggcaucc accagauuuu cggagccgcu uuuaaaagcc     1980

uguucggcgg gauguccugg uuuucucaaa uccuuaucgg aacacuccug auguggcugg     2040

gccucaacac gaagaauggg agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca     2100

ucuuccugag caccgccguu ucugcuugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6701
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6701
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu gggcaucguc gggcugcucc ugacaacggc uauggccgcg gagguuacca      120

gacggggauc cgcuuacuau auguaccuug aucgcaacga cgccggcgaa gccauuucuu      180

uccccacaac ccuggggaug aauaagugcu auauccagau uauggaucuc ggacacaugu      240

gugacgcuac aaugagcuac gagugcccua ugcuggauga aggcguggag ccggacgaug      300

uggacuguug gugcaacacc acauccaccu gggucgugua ugggacgugu caucacaaaa      360

agggagaagc cagacggucu cgcagagcug uuacccuucc cagccauucc acacggaaac      420

ugcaaacccg cucucagaca uggcucgaga gcagagagua caccaagcac cugauccggg      480

uggaaaauug gauuuuucgc aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc      540

uccugggcuc cagcacgucu cagaaaguca ucuaucuugu gaugauucug cucaucgccc      600

ccgcguacuc cauuagaugc aucgggguua gcaaucggga uuuuguggag ggaaugucug      660

gcgggacaug ggucgacauu gugcuggaac auggaggcug ugugaccguc auggcccagg      720

auaagccuac aguugacauc gagcuuguga ccaccacagu guccaacaug gcugaggucc      780

gcagcuacug cuaugaagcc ucuauuuccg auauggcuag cgacuccaga ugucccacgc      840

aaggggaggc cuaccuggac aagcagucug auacccagua ugugugcaaa cggacacucg      900

uugaccgcgg auggggcaau ggcugugggc uguucggaaa gggcagccug gugaccugcg      960

ccaaauuugc uugcuccaag aaaaugacag gaaagagcau ccagccugaa aaccucgagu     1020

acagaaucau gcugucuguc cacggguccc aacacagcgg caugauugug aaugauaccg     1080

ggcaugaaac ggacgagaac cgggcgaagg uugagaucac acccaauucu cccagagccg     1140

aagcuacccu uggaggcuuc ggcagccugg ggcucgauug ugagccucgc accggacugg     1200

acuuuuccga ucuuuauuac cugacaauga acaauaaaca cuggcucgug cauaaggaau     1260

gguuccacga cauuccccug ccuuggcacg ccggcgccga uaccgggaca ccccauugga     1320

acaauaaaga ggcucugguc gaauuuaagg acgcccacgc gaaacggcag acgguggucg     1380

ugcucggauc ucaggagggc gcuguucaua ccgcccuggc cggggcucuu gaggccgaaa     1440

uggauggcgc uaagggaaga cugagcuccg gccaccucaa augccgccug aagauggaca     1500

agcugcggcu gaaaggggug agcuauucuc ucuguacagc cgcguucacc uuuacaaaga     1560

ucccggccga gacccugcau ggaacgguca ccguggaagu ucaguacgcu ggcacagacg     1620

ggccuugcaa agugcccgcc caaauggcug ucgauaugca gacccugaca ccugugggaa     1680

gacucauuac cgccaacccc gugaucacgg aguccacaga aaacagcaag augaugcugg     1740

agcuugaccc gccuuucggc gauucuuaca ucguuauugg ggucggcgag aaaaagauca     1800

cccaccacug gcaucgcucc ggaagcacaa uuggcaaggc guuugaagcc accgugagag     1860

gggcuaaacg gauggccgug cugggagaca ccgcuuggga uuucggcucc gucgggggag     1920

cccucaauuc ucugggcaag gggauccacc agauuuucgg cgccgcuuuu aaaagccugu     1980

ucggaggcau guccugguuu ucucaaaucc uuaucgggac acuccugaug uggcugggac     2040

ucaacacgaa gaauggcagc auuucccuga ugugucuugc gcuuggggga gugcucaucu     2100

uccugagcac cgccguuucu gcuugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  6702
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6702
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccagcguggg caucgucggg cugcuccuga caacggccau ggcugccgag guuaccagac      120

ggggauccgc guacuauaug uaccuugauc gcaacgacgc uggcgaagcc auuucuuucc      180

ccacaacccu ggggaugaau aagugcuaua uccagauuau ggaucucgga cacaugugug      240

acgccacaau gagcuacgag ugcccuaugc uggaugaagg cguggagccg gacgaugugg      300

acuguuggug caacaccaca uccaccuggg ucguguaugg gacgugucau cacaaaaagg      360

gagaagcuag acggucucgc agagccguua cccuucccag ccauuccaca cggaaacugc      420

aaacccgcuc ucagacaugg cucgagagca gagaguacac caagcaccug auccgggugg      480

aaaauuggau uuuucgcaac ccuggcuucg cucuggccgc ggccgcuauc gccuggcucc      540

ugggcuccag cacgucucag aaagucaucu aucuugugau gauucugcuc aucgcucccg      600

ccuacuccau uagaugcauc gggguuagca aucgggauuu uguggaggga augucuggcg      660

ggacaugggu cgacauugug cuggaacaug gaggcugugu gaccgucaug gcgcaggaua      720

agccuacagu ugacaucgag cuugugacca ccacaguguc caacauggcc gagguccgca      780

gcuacugcua ugaagcuagc auuuccgaua uggccagcga cuccagaugu cccacgcaag      840

gggaggcuua ccuggacaag cagucugaua cccaguaugu gugcaaacgg acacucguug      900

accgcggaug gggcaauggc ugugggcugu ucggaaaggg cagccuggug accugcgcca      960

aauuugccug cuccaagaaa augacaggga agucuaucca gccugaaaac cucgaguaca     1020

gaaucaugcu gucuguccac ggaucccaac acagcggcau gauugugaau gauaccgggc     1080

augaaacgga cgagaaccgg gcuaagguug agaucacacc caauucuccc agagcggaag     1140

ccacccuugg aggcuucggc agccuggggc ucgauuguga gccucgcacc ggacuggacu     1200

uuuccgaucu uuauuaccug acaaugaaca auaaacacug gcucgugcau aaggaauggu     1260

uccacgacau uccccugccu uggcacgcug gcgccgauac cgggacaccc cauuggaaca     1320

auaaagaggc ccuggucgaa uuuaaggacg cucacgccaa acggcagacg guggucgugc     1380

ucggaucuca ggagggcgcg guucauaccg cucuggccgg ggcccuugag gcugaaaugg     1440

auggcgccaa gggaagacug agcuccggcc accucaaaug ccgccugaag auggacaagc     1500

ugcggcugaa aggggugagc uauucucucu guacagcugc cuucaccuuu acaaagaucc     1560

cggcggagac ccugcaugga acggucaccg uggaaguuca guacgccggc acagacgggc     1620

cuugcaaagu gcccgcucaa auggccgucg auaugcagac ccugacaccu gugggaagac     1680

ucauuaccgc uaaccccgug aucacggagu ccacagaaaa cagcaagaug augcuggagc     1740

uugacccgcc uuucggcgau ucuuacaucg uuauuggggu cggcgagaaa aagaucaccc     1800

accacuggca ucgcuccgga agcacaauug gcaaggccuu ugaagcgacc gugagagggg     1860

ccaaacggau ggcugugcug ggagacaccg ccugggauuu cggcuccguc gggggagcuc     1920

ucaauucucu gggcaagggg auccaccaga uuuucggcgc cgccuuuaaa agccuguucg     1980

gaggcauguc cugguuuucu caaauccuua ucgggacacu ccugaugugg cugggacuca     2040

acacgaagaa uggcagcauu ucccugaugu gucuugcucu ugggggagug cucaucuucc     2100

ugagcaccgc gguuucugcc ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6703
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  6703
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6704
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6704
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgcgguu accagacggg      120

gauccgcuua cuauauguac cuugaccgca acgaugccgg cgaggccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

cuacaaugag cuacgaaugc ccuaugcugg acgagggcgu ggaaccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aggccagacg gucucgcaga gcuguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gaaagcagag aguacaccaa gcaccugauc cggguggaga      480

auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcccccgcgu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggaaggaaug ucuggcggga      660

caugggucga uauugugcug gagcauggag gcugugugac cgucauggcc caggacaagc      720

cuacaguuga uaucgaacuu gugaccacca caguguccaa cauggcugag guccgcagcu      780

acugcuauga ggccucuauu uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aagccuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugcuugcuc caagaaaaug acagggaagu cuauccagcc ugagaaccuc gaauacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

agacggauga aaaccgggcg aagguugaga ucacaccgaa uagccccaga gccgaggcua     1140

cccuuggagg cuucggcagc cuggggcucg acugugaacc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gagugguucc     1260

acgauauucc ccugccuugg cacgccggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaagcucu ggucgaguuu aaggaugccc acgcgaaacg gcagacggug gucgugcucg     1380

gaucucagga aggcgcuguu cauaccgccc uggccggggc ucuugaggcc gagauggacg     1440

gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca aagaucccgg     1560

ccgaaacccu gcauggaacg gucaccgugg agguucagua cgcuggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcugucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgccaa ccccgugauc acggaaucca cagagaacag caagaugaug cuggaacuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggcguuuga ggccaccgug agaggggcua     1860

aacggauggc cgugcuggga gauaccgcuu gggacuucgg cuccgucggg ggagcccuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc aucuuccuga     2100

gcaccgccgu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6705
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6705
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgacac cagcgugggg aucgucggac ugcuccugac aacggcuaug gccgcggcug      120

uuacccggcg cggcuccgcc uacuauaugu accuugauag aaacgacgcc ggggaggcua      180

uuucuuuccc cacaacccug ggaaugaaua agugcuauau ccagauuaug gaucucggcc      240

acauguguga cgccacaaug agcuacgaau gcccuaugcu ggaugagggg guggaaccgg      300

acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga acgugucauc      360

acaaaaaggg cgaggcucgg cgcucuagac gggccguuac ccuucccagc cauuccacac      420

gcaaacugca aaccagaucu cagacauggc ucgaaagccg ggaguacacc aagcaccuga      480

uccgcgugga gaauuggauu uuuagaaacc cuggcuucgc gcuggccgcu gccgcuaucg      540

ccuggcuccu gggguccagc acgucucaga aagucaucua ucuugugaug auucugcuca      600

ucgcgcccgc cuacuccauu cggugcaucg gaguuagcaa ucgcgauuuu guggaaggca      660

ugucuggggg aacauggguc gacauugugc uggagcaugg cgggugugug accgucaugg      720

cucaggauaa gccuacaguu gacaucgaac uugugaccac cacagugucc aacauggccg      780

aggucagaag cuacugcuau gaggcuucua uuuccgauau ggccagcgac ucccgguguc      840

ccacgcaagg agaagccuac cuggacaagc agucugauac ccaguaugug ugcaaacgca      900

cacucguuga cagaggcugg ggcaaugggu guggacuguu cggcaagggg agccugguga      960

ccugcgcuaa auuugcgugc uccaagaaaa ugacaggaaa gagcauccag ccugagaacc     1020

ucgaauaccg gaucaugcug ucuguccacg gcucccaaca cagcgggaug auugugaaug     1080

auaccggaca ugagacggac gaaaacagag ccaagguuga gaucacaccc aauucucccc     1140

gcgcugaggc cacccuuggc ggcuucggga gccugggacu cgauugugaa ccucggaccg     1200

gccuggacuu uuccgaucuu uauuaccuga caaugaacaa uaaacacugg cucgugcaua     1260

aggagugguu ccacgacauu ccccugccuu ggcacgccgg ggcugauacc ggaacacccc     1320

auuggaacaa uaaagaagcc cuggucgagu uuaaggacgc gcacgcuaaa agacagacgg     1380

uggucgugcu cggcucucag gaaggggccg uucauaccgc ccuggcuggc gcccuugagg     1440

cugagaugga uggagccaag ggccgccuga gcuccgggca ccucaaaugc cggcugaaga     1500

uggacaaacu uagacugaag ggagugagcu auucucucug uacagcggcc uucaccuuua     1560

caaagauccc ggcugaaacc cugcauggca cggucaccgu ggagguucag uacgccggga     1620

cagacggacc uugcaaagug cccgcucaaa uggccgucga uaugcagacc cugacaccug     1680

ugggccgccu cauuaccgcg aaccccguga ucacggaauc cacagagaac agcaagauga     1740

ugcuggaacu ugacccgccu uucggggauu cuuacaucgu uauuggcguc ggagagaaaa     1800

agaucaccca ccacuggcau cgguccggca gcacaauugg gaaggccuuu gaggcuaccg     1860

ugagaggagc caaacgcaug gcugugcugg gcgacaccgc cugggauuuc ggguccgucg     1920

gaggcgcccu caauucucug gggaagggca uccaccagau uuucggagcu gcguuuaaaa     1980

gccuguucgg cgggaugucc ugguuuucuc aaauccuuau cggaacacuc cugauguggc     2040

ugggccucaa cacgaagaau gggagcauuu cccugaugug ucuugcccug ggaggcgugc     2100

ucaucuuccu gagcaccgcu guuucugccu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6706
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6706
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc ccggaagcgc agaucucacg acgugcucac cguccaguuc cugauccuug      120

ggaugcugcu caugacagga ggcguuacgc ggcgcgggag cgccuacuau auguaccugg      180

auagaaacga cgcuggagag gccauuuccu uucccaccac acuuggcaug aauaaaugcu      240

auauccaaau uauggaucug gggcauaugu gugacgcgac caugucuuac gaaugcccua      300

ugcucgauga gggaguggaa ccggacgaug uggacuguug gugcaacaca accagcacau      360

gggucgugua uggcaccugu caccauaaga aaggcgaggc ucggcgcucc agacgggccg      420

uuacgcugcc cucucacagc acccgcaagc ugcagacaag aucccagacc uggcucgaaa      480

gccgggagua cacaaaacau cugauccgcg uggagaauug gauuuucaga aacccugggu      540

uugcccuugc ugccgcugcc aucgcguggc ugcucggauc uuccaccagc cagaagguca      600

ucuaucuggu gaugauucuu cugaucgccc ccgcuuacuc uauucggugc aucggcguuu      660

ccaaucgcga uuucguggaa gggaugagcg gaggcacgug ggucgacauu gugcucgagc      720

acgggggaug ugugacaguc auggcccaag auaagccuac cguugacauc gaacugguga      780

caaccaccgu gucuaacaug gcugagguca gauccuacug cuaugaggcc agcauuuccg      840

auauggcguc ugacagccgg ugucccacac agggcgaagc cuaccuggac aaacaguccg      900

auacgcagua ugugugcaag agaacccucg uugaccgcgg cugggggaau ggauguggcc      960

uguuugggaa aggaagccuu gugacaugcg cuaaguucgc cugcucuaaa aagaugaccg     1020

gcaaguccau ccaaccugag aaccuggaau accggaucau gcucagcguc cacgggucuc     1080

agcauagcgg aaugauugug aaugauacag gccacgagac cgacgaaaac agagcuaaag     1140

uugagaucac gcccaauucc ccgcgcgccg aggccacacu gggcggguuu ggaucucuug     1200

gccuggauug ugaaccucgg accgggcucg acuucagcga ucuguauuac cugaccauga     1260

acaauaagca uuggcucgug cacaaagagu gguuucacga cauuccccug ccuuggcaug     1320

cuggagcgga uacaggcaca ccccacugga acaauaagga agcccuuguc gaguucaaag     1380

acgcucaugc caagagacag acaguggucg ugcugggguc ccaggaaggc gccguucaca     1440

cggcucucgc cggagcgcug gaggcugaga uggauggcgc caaagggcgc cuuagcucug     1500

gacaucugaa gugccggcuc aagauggaca aacugagacu gaagggcgug uccuauagcc     1560

ucuguaccgc cgcuuuuaca uucaccaaaa ucccggccga aacacugcac gggaccguca     1620

cgguggaggu ucaauacgcu ggaaccgacg gcccuugcaa ggugcccgcc cagauggcgg     1680

ucgauaugca gacacuuacc ccuguggggc gccugauuac agccaacccc gugaucaccg     1740

aaucuacgga gaacuccaaa augaugcucg aacuggaucc cccuuuuggc gacagcuaca     1800

ucguuauugg agucggcgag aagaagauca cacaccauug gcaccggucc gggucuacca     1860

uuggaaaggc uuucgaggcc acagugagag gcgcuaaacg cauggccgug cugggggaca     1920

ccgcguggga uuucggaagc gucggcgggg cccuuaauuc ccucggcaaa ggaauccauc     1980

aaauuuuugg cgcugccuuc aagucucugu uugggggaau gagcugguuc ucccagaucc     2040

ugaucggcac ccuccugaug uggcuugggc ugaacaccaa aaauggaagc auuucucuca     2100

ugugucuggc ucugggcggg gugcuuaucu uucucuccac ggccguuagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6707
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6707
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccugagcuc ccggaagcgc agaucucacg acgugcucac cguccaguuc cugauccuug      120

ggaugcugcu caugacagga gccguuacgc ggcgcggcag cgcuuacuau auguaccugg      180

auagaaacga cgccggggag gcgauuuccu uucccaccac acuuggaaug aauaaaugcu      240

auauccaaau uauggaucug ggccauaugu gugacgcuac caugucuuac gaaugcccua      300

ugcucgauga ggggguggaa ccggacgaug uggacuguug gugcaacaca accagcacau      360

gggucgugua uggaaccugu caccauaaga aaggcgaggc ccggcgcucc agacgggccg      420

uuacgcugcc cucucacagc acccgcaagc ugcagacaag aucccagacc uggcucgaaa      480

gccgggagua cacaaaacau cugauccgcg uggagaauug gauuuucaga aacccuggcu      540

uugcucuugc cgcugccgcg aucgccuggc ugcucggguc uuccaccagc cagaagguca      600

ucuaucuggu gaugauucuu cugaucgcuc ccgccuacuc uauucggugc aucggaguuu      660

ccaaucgcga uuucguggaa ggcaugagcg ggggaacgug ggucgacauu gugcucgagc      720

acggcgggug ugugacaguc auggcucaag auaagccuac cguugacauc gaacugguga      780

caaccaccgu gucuaacaug gccgagguca gauccuacug cuaugaggcg agcauuuccg      840

auauggccuc ugacagccgg ugucccacac agggagaggc uuaccuggac aaacaguccg      900

auacgcagua ugugugcaag agaacccucg uugaccgcgg cuggggcaau ggguguggac      960

uguuuggcaa agggagccuu gugacaugcg ccaaguucgc uugcucuaaa aagaugaccg     1020

gaaaguccau ccaaccugaa aaccuggaau accggaucau gcucagcguc cacggcucuc     1080

agcauagcgg gaugauugug aaugauacag gacacgagac cgacgaaaac agagccaaag     1140

uugagaucac gcccaauucc ccgcgcgccg aggcuacacu gggcggcuuu gggucucuug     1200

gacuggauug ugaaccucgg accggccucg acuucagcga ucuguauuac cugaccauga     1260

acaauaagca uuggcucgug cacaaagagu gguuucacga cauuccccug ccuuggcaug     1320

cgggggccga uacaggaaca ccccacugga acaauaagga agcucuuguc gaguucaaag     1380

acgcccaugc caagagacag acaguggucg ugcugggcuc ccaggaaggg gcuguucaca     1440

cggcccucgc gggcgcucug gaggccgaga uggauggagc caaaggccgc cuuagcucug     1500

ggcaucugaa gugccggcuc aagauggaca aacugagacu gaagggagug uccuauagcc     1560

ucuguaccgc ugccuuuaca uucaccaaaa ucccggcuga aacacugcac ggcaccguca     1620

cgguggaggu ucaauacgcc gggaccgacg gaccuugcaa ggugcccgcg cagauggccg     1680

ucgauaugca gacacuuacc ccugugggcc gccugauuac agcuaacccc gugaucaccg     1740

aaucuacgga gaacuccaaa augaugcucg aacuggaucc cccuuuuggg gacagcuaca     1800

ucguuauugg cgucggagag aagaagauca cacaccauug gcaccggucc ggcucuacca     1860

uugggaaagc cuucgaggcu acagugagag gagccaagcg cauggcggug cugggcgaca     1920

ccgccuggga uuucgggagc gucggaggcg cucuuaauuc ccucgggaaa ggcauccauc     1980

aaauuuuugg agccgcuuuc aagucucugu uuggcgggau gagcugguuc ucccagaucc     2040

ugaucggaac ccuccugaug uggcuuggcc ugaacaccaa aaaugggagc auuucucuca     2100

ugugucuggc ccugggaggc gugcuuaucu uucucuccac ggccguuagc gcuugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6708
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 2 opt

<400>  6708
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacauug      720

ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca guugacaucg      780

agcuugugac caccacagug uccaauaugg ccgaagucag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ugaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcgcacgcu aagagacaga cgguggucgu gcucggcucu caggaggggg     1440

ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

ugauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc gcuguuucug     2160

ccgacguggg gugcuccguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6709
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 2 opt

<400>  6709
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacacaug ugacgccacc augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacaa      360

ccuccacgug ggucguguau ggaaccuguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacacuuccc agccauucca cccgcaagcu gcaaacaaga ucucagaccu      480

ggcucgagag ccgggaauac acgaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacaucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaaccugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcucagga uaagccuacc guggacauug      780

agcuugugac cacaacgguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccaccca aggagaagcc uaccuggaca      900

aacagucuga uacacaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ucacaugcgc uaaguuugcg ugcuccaaga     1020

aaaugaccgg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugugc     1080

acggcuccca acacagcggg augaucguua acgauacggg acaugagaca gacgagaaua     1140

gagccaaagu ggaaauuacc ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga caggccugga cuuuuccgau cuuuauuacc     1260

ugaccaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau acaggaacgc cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga ccgucguggu ucucggcucu caggaggggg     1440

ccgugcauac agcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguaccgcg gccuucacau uuaccaaaau uccggcugaa acgcugcaug     1620

gcaccgugac aguugaggug caguacgccg ggaccgacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acacugaccc cugugggccg ccucaucacg gcgaaccccg     1740

uuaucacaga auccaccgag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac agccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaacg cuccugaugu ggcugggccu caaugccaag aacgggagca     2100

ucucccugau gugucuugcu cugggaggcg uucucauuuu ccugagcacc gccgugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6710
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 2 opt

<400>  6710
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc cugggaguga      240

auaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu gacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugauuaaagu ugaaaauugg aucuuuagaa      540

accccggcuu cgcccuggug gcugucgcca uugcuuggcu ccuggggagc ucuacguccc      600

agaaggugau cuaccuuguu augauucugc ucaucgcccc ugcguauagc auucggugca      660

ucggaguguc uaaucgcgau uuugucgagg gcauguccgg gggaacaugg guggacgugg      720

uccuggaaca cggcgggugu guuaccguga uggcccagga uaaacccaca guggacaucg      780

agcuugucac caccacagug agcaacaugg cugagguuag aucuuacugc uacgaagccu      840

ccauuagcga uauggcuucc gacucucggu guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gugugcaaaa gaacacucgu cgaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg ugaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauc cagcccgaaa accucgagua ucggauuaug cuguccguuc     1080

acggcagcca acauucuggg augaucggau acgaaacgga ugaggacaga gcuaaggugg     1140

aggucacacc uaauagcccg cgcgcggaag ccacccuugg cggcuucggg ucccugggac     1200

ucgauuguga gccccggacc ggccuggacu uuucugaucu uuauuaccug acaaugaaca     1260

auaaacacug gcucgugcau aaggaauggu uccacgacau uccucugccc uggcacgcug     1320

gggccgauac cggaacaccu cauuggaaca auaaagaggc ccuggucgaa uuuaaggacg     1380

cucacgccaa gagacagacg gugguugugc ucggcagcca ggagggggcg guccauaccg     1440

cucuggccgg cgcccuugag gcugaaaugg auggagccaa aggccgccug uucuccgggc     1500

accucaagug ccggcugaaa auggacaaac uuagacugaa gggagugagc uauucucucu     1560

guacagcugc cuuuaccuuc acaaagguuc ccgcggagac ccugcauggc acggugaccg     1620

ucgaagugca guacgccggg acagacggac cgugcaagau cccugugcaa auggcuguug     1680

auaugcagac ccugacaccc gucggccgcc ucaucaccgc caacccugug auuacggagu     1740

ccacagaaaa uagcaaaaug augcuggagc uugacccucc cuuuggggau ucuuacaucg     1800

ugauuggcgu cggagacaag aaaaucaccc accacuggca ucgguccggc agcacaauug     1860

ggaaggcuuu cgaggccacc gugagaggag cgaaacgcau ggccguucug ggcgauaccg     1920

cuugggacuu cggguccgug ggaggcgucu uuaacucucu cgggaagggc auccaccaga     1980

ucuucggagc cgcuuuuaag agccuguucg gcgggauguc cugguuuucu caaauucuga     2040

ucggaacacu ucucgugugg cugggccuga acacgaaaaa ugggagcauu ucccucaccu     2100

gucuggcccu uggaggcguu augaucuucc ugagcacagc cgugucugcu gaugucgggu     2160

gcuccgugga cuuuagcaag ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6711
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 2 opt

<400>  6711
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6712
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 2 opt

<400>  6712
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc cugggaguga      240

auaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu gacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugauuaaagu ugaaaauugg aucuuuagaa      540

accccggcuu cgcccuggug gcugucgcca uugcuuggcu ccuggggagc ucuacguccc      600

agaaggugau cuaccuuguu augauucugc ucaucgcccc ugcguauagc auucggugca      660

ucggaguguc uaaucgcgau uuugucgagg gcauguccgg gggaacaugg guggacgugg      720

uccuggaaca cggcgggugu guuaccguga uggcccagga uaaacccaca guggacaucg      780

agcuugucac caccacagug agcaacaugg cugagguuag aucuuacugc uacgaagccu      840

ccauuagcga uauggcuucc gacucucggu guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gugugcaaaa gaacacucgu cgaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg ugaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauc cagcccgaaa accucgagua ucggauuaug cuguccguuc     1080

acggcagcca acauucuggg augaucguga augauacggg auacgaaaca gacgagaaca     1140

gagcuaaggu cgaggugacc ccuaauagcc cgcgcgcgga agccacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gagccccgga caggccugga cuuuucugau cuuuauuacc     1260

ugaccaugaa caauaaacac uggcucgucc auaaggaaug guuccacgac auuccucugc     1320

ccuggcacgc uggggccgau acaggaacgc cucauuggaa caauaaagag gcccuggugg     1380

aauuuaagga cgcucacgcc aagagacaga ccguuguggu ccucggcagc caggaggggg     1440

cggugcauac agcucuggcc ggcgcccuug aggcugaaau ggauggagcc aaaggcaagc     1500

uguucuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug aagggaguua     1560

gcuauucucu cuguaccgcu gccuuuacau ucaccaaggu gcccgcggag acgcugcaug     1620

gcaccgucac aguggaagug caguacgccg ggaccgacgg accgugcaaa aucccuguuc     1680

aaauggcugu cgauaugcag acacugaccc ccgugggcag acucaucacg gccaacccug     1740

ugauuacaga guccaccgaa aacagcaaga ugaugcugga gcuugacccu cccuuugggg     1800

auucuuacau cgucauuggc gugggagaca aaaagaucac acaccacugg caucgcuccg     1860

gcagcaccau ugggaaggcu uucgaggcca ccguucgggg agcgaaaaga auggccgugc     1920

ugggcgauac agcuugggac uucggguccg ucggaggcgu guuuaauucu cucgggaagg     1980

gcauccacca gaucuucgga gccgcuuuua aaagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu gaucggaacg cuucucguuu ggcugggccu gaacaccaag aaugggagca     2100

uuucccucac augucuggcc cuuggaggcg ugaugaucuu ccugagcacc gccgucucug     2160

cugauguggg gugcuccguu gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6713
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 2 opt

<400>  6713
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauugugg      120

gacugcuccu gacaacggcc auggcggcug aaaucacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuauuagcuu cgccacaacc cugggaguca      240

auaaaugcca cguucagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguggagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug ggucguguac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu uacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugaucaaagu ggaaaauugg auuuuuagaa      540

accccggcuu cacgcugguc gccguggcua ucgccuggcu ccuggggagc ucuacauccc      600

agaagguuau uuaccuugug augauccugc ucauugcucc ugccuauagc auccggugca      660

uuggagucuc uaaucgcgau uuuguggagg gcauguccgg gggaaccugg guggacgucg      720

uucuggaaca cggcgggugu gugacaguga uggcgcagga uaaacccacc gucgacaucg      780

agcuugugac cacaacgguu agcaacaugg ccgaggugag aucuuacugc uacgaagccu      840

ccaucagcga uauggcuucc gacucucggu guccuaccca aggagaggcu uaucuggaca      900

agcagagcga uacacaguac gucugcaaaa gaacccucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg uuacaugcgc caaguuuacc ugcagcaaaa     1020

agaugacggg aaaaucuauu cagcccgaaa accucgagua ucggaucaug cuguccgugc     1080

acggcagcca acauucuggg augauuguca augaugaaaa cagagccaag guggagguca     1140

caccuaauag cccgcgcgcu gaggcgaccc uuggaggcuu cggcucccug gggcucgacu     1200

gugaaccccg gaccggacug gauuuuucug accuuuacua ucugacaaug aacaauaaac     1260

acuggcucgu gcauaaggag ugguuccacg auaucccucu gcccuggcac gccggcgcug     1320

acaccgggac accucauugg aacaauaaag aagcccuggu ugaguuuaag gaugcccacg     1380

cuaagagaca gacggugguc gugcucggaa gccaggaagg cgccguucau accgcgcugg     1440

cuggggcccu ugaggccgag auggacggcg cuaaaggacg ccuguucucc ggccaccuca     1500

agugccggcu gaaaauggau aaacuuagac ugaagggggu gagcuacucu cucuguacag     1560

ccgcuuuuac cuucacaaag guccccgccg aaacccugca uggaacggug accguggagg     1620

uucaguaugc gggcacagac gggccgugca aggucccugc ccaaauggcu guggacaugc     1680

agacccugac acccguggga cgccucauua ccgccaaccc ugucaucacg gaauccacag     1740

agaacagcaa aaugaugcug gaacuugauc cucccuuugg cgacucuuac aucgugauug     1800

ggguuggcga uaagaaaauc acccaccacu ggcaucgguc cggaagcaca auuggcaagg     1860

cuuucgaggc caccgugaga ggggcgaaac gcauggccgu ccugggagac accgcuuggg     1920

auuucggcuc cguuggggga guguuuaauu cucucggcaa ggggauccac cagauuuucg     1980

gcgccgcuuu uaagagccug uucggaggca uguccugguu uucucaaauc cugaucggga     2040

cacuucucgu guggcuggga cugaacacga aaaauggcag cauuucccuc accugucugg     2100

cccuuggggg agucaugauc uuccugagca cagccguguc ugcugacguu ggcugcuccg     2160

uggauuuuag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6714
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 2 opt

<400>  6714
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc cugggaguga      240

auaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accgcaagaa aggcgaagcc agacggagcc      420

gcagagcggu gacccuuccg ucccauucua cacggaagcu gcaaacccgc agccagacau      480

ggcucgaguc cagagaguau accaagcacc ugauuaaagu ugaaaauugg aucuuucgga      540

accccggcuu cgcccuggug gcugucgcca uugcuuggcu ccuggggagc ucuacguccc      600

agaaggugau cuaccuuguu augauucugc ucaucgcccc ugcguauagc auucgcugca      660

ucggaguguc uaauagagau uuugucgagg gcauguccgg gggaacaugg guggacgugg      720

uccuggaaca uggcgggugu guuaccguga uggcccagga uaaacccaca guggacaucg      780

agcuugucac caccacagug agcaacaugg cugagguucg gucuuacugc uacgaagccu      840

ccauuagcga uauggcuucc gacucuagau guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gugugcaaac gcacacucgu cgaccggggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg ugaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauc cagcccgaaa accucgagua uagaauuaug cuguccguuc     1080

acggcagcca acacucuggg augaucguga augauacggg auacgaaaca gacgagaacc     1140

gcgcuaaggu cgaggugacc ccuaauagcc cgcgggcgga agccacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gagcccagaa caggccugga cuuuucugau cuuuauuacc     1260

ugaccaugaa caauaaacau uggcucgucc acaaggaaug guuccaugac auuccucugc     1320

ccuggcacgc uggggccgau acaggaacgc cucacuggaa caauaaagag gcccuggugg     1380

aauuuaagga cgcucaugcc aagcgccaga ccguuguggu ccucggcagc caggaggggg     1440

cggugcacac agcucuggcc ggcgcccuug aggcugaaau ggauggagcc aaaggcaagc     1500

uguucuccgg gcaucucaaa ugccggcuga agauggacaa acuuagacug aagggaguua     1560

gcuauucucu cuguaccgcu gccuuuacau ucaccaaggu gcccgcggag acgcugcacg     1620

gcaccgucac aguggaagug caguacgccg ggaccgacgg accgugcaaa aucccuguuc     1680

aaauggcugu cgauaugcag acacugaccc ccgugggccg ccucaucacg gccaacccug     1740

ugauuacaga guccaccgaa aacagcaaga ugaugcugga gcuugacccu cccuuugggg     1800

auucuuacau cgucauuggc gugggagaca aaaagaucac acaucacugg caccgguccg     1860

gcagcaccau ugggaaggcu uucgaggcca ccguuagagg agcgaaacgc auggccgugc     1920

ugggcgauac agcuugggac uucggguccg ucggaggcgu guuuaauucu cucgggaagg     1980

gcauccauca gaucuucgga gccgcuuuua aaagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu gaucggaacg cuucucguuu ggcugggccu gaacaccaag aaugggagca     2100

uuucccucac augucuggcc cuuggaggcg ugaugaucuu ccugagcacc gccgucucug     2160

cugauguggg gugcuccguu gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6715
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 2 opt

<400>  6715
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc cugggaguga      240

auaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu gacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugauuaaagu ugaaaauugg aucuuuagaa      540

accccggcuu cgcccuggug gcugucgcca uugcuuggcu ccuggggagc ucuacguccc      600

agaaggugau cuaccuuguu augauucugc ucaucgcccc ugcguauagc auucggugca      660

ucggaguguc uaaucgcgau uuugucgagg gcauguccgg gggaacaugg guggacgugg      720

uccuggaaca cggcgggugu guuaccguga uggcccagga uaaacccaca guggacaucg      780

agcuugucac caccacagug agcaacaugg cugagguuag aucuuacugc uacgaagccu      840

ccauuagcga uauggcuucc gacucucggu guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gugugcaaaa gaacacucgu cgaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg ugaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauc cagcccgaaa accucgagua ucggauuaug cuguccguuc     1080

acggcagcca acauucuggg augacgguga augauaucgg auacgaaaca gacgagaaca     1140

gagcuaaggu cgaggugacc ccuaauagcc cgcgcgcgga agccacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gagccccgga caggccugga cuuuucugau cuuuauuacc     1260

ugaccaugaa caauaaacac uggcucgucc auaaggaaug guuccacgac auuccucugc     1320

ccuggcacgc uggggccgau acaggaacgc cucauuggaa caauaaagag gcccuggugg     1380

aauuuaagga cgcucacgcc aagagacaga ccguuguggu ccucggcagc caggaggggg     1440

cggugcauac agcucuggcc ggcgcccuug aggcugaaau ggauggagcc aaaggcaagc     1500

uguucuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug aagggaguua     1560

gcuauucucu cuguaccgcu gccuuuacau ucaccaaggu gcccgcggag acgcugcaug     1620

gcaccgucac aguggaagug caguacgccg ggaccgacgg accgugcaaa aucccuguuc     1680

aaauggcugu cgauaugcag acacugaccc ccgugggcag acucaucacg gccaacccug     1740

ugauuacaga guccaccgaa aacagcaaga ugaugcugga gcuugacccu cccuuugggg     1800

auucuuacau cgucauuggc gugggagaca aaaagaucac acaccacugg caucgcuccg     1860

gcagcaccau ugggaaggcu uucgaggcca ccguucgggg agcgaaaaga auggccgugc     1920

ugggcgauac agcuugggac uucggguccg ucggaggcgu guuuaauucu cucgggaagg     1980

gcauccacca gaucuucgga gccgcuuuua aaagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu gaucggaacg cuucucguuu ggcugggccu gaacaccaag aaugggagca     2100

uuucccucac augucuggcc cuuggaggcg ugaugaucuu ccugagcacc gccgucucug     2160

cugauguggg gugcuccguu gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6716
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 2 opt

<400>  6716
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc cugggaguga      240

auaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu gacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugauuaaagu ugaaaauugg aucuuuagaa      540

accccggcuu cgcccuggug gcugucgcca uugcuuggcu ccuggggagc ucuacguccc      600

agaaggugau cuaccuuguu augauucugc ucaucgcccc ugcguauagc auucggugca      660

ucggaguguc uaaucgcgau uuugucgagg gcauguccgg gggaacaugg guggacgugg      720

uccuggaaca cggcgggugu guuaccguga uggcccagga uaaacccaca guggacaucg      780

agcuugucac caccacagug agcaacaugg cugagguuag aucuuacugc uacgaagccu      840

ccauuagcga uauggcuucc gacucucggu guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gugugcaaaa gaacacucgu cgaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg ugaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauc cagcccgaaa accucgagua ucggauuaug cuguccguuc     1080

acggcagcca acauucuggg augaucggau acgaaacgga ugagaauaga gcuaaggugg     1140

aggucacacc uaacagcccg cgcgcggaag ccacccuugg cggcuucggg ucccugggac     1200

ucgacuguga gccccggacc ggccuggauu uuucugaccu uuauuaccug acaaugaaua     1260

acaaacacug gcucgugcau aaggaauggu uccacgauau uccucugccc uggcacgcug     1320

gggccgacac cggaacaccu cauuggaaua acaaagaggc ccuggucgaa uuuaaggaug     1380

cucacgccaa gagacagacg gugguugugc ucggcagcca ggagggggcg guccauaccg     1440

cucuggccgg cgcccuugag gcugaaaugg acggagccaa aggcaagcug uucuccgggc     1500

accucaaaug ccgccugaag auggauaaac uucggcugaa gggagugagc uauucucucu     1560

guacagcugc cuuuaccuuc acaaagguuc ccgcggagac ccugcauggc acggugaccg     1620

ucgaagugca guacgccggg acagacggac cgugcaaaau cccugugcaa auggcuguug     1680

acaugcagac ccugacaccc gucggcagac ucaucaccgc caauccugug auuacggagu     1740

ccacagaaaa cagcaagaug augcuggagc uugauccucc cuuuggggac ucuuacaucg     1800

ugauuggcgu cggagauaaa aagaucaccc accacuggca ucgcuccggc agcacaauug     1860

ggaaggcuuu cgaggccacc gugcggggag cgaaaagaau ggccguucug ggcgacaccg     1920

cuugggauuu cggguccgug ggaggcgucu uuaacucucu cgggaagggc auccaccaga     1980

ucuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu caaauucuga     2040

ucggaacacu ucucgugugg cugggccuga auacgaagaa cgggagcauu ucccucaccu     2100

gucuggcccu uggaggcguu augaucuucc ugagcacagc cgugucugcu gacgucgggu     2160

gcuccgugga uuuuagcaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6717
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 2 opt

<400>  6717
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggcggg     1980

gcauucacca gaucuucgga gcugcguuua aaagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6718
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 2 opt

<400>  6718
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga cgccagcgug gggaucgucg      120

gacugcuccu gaccacagcg auggcugccg aaguuacgcg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gcuggggagg ccauuucuuu ccccaccaca cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgcuacc augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacaa      360

ccuccacaug ggucguguau ggaaccuguc aucacaagaa aggcgaggcc cggcgcucua      420

gacgggcggu uacgcuuccc agccauucca cccgcaagcu gcaaacaaga ucucagaccu      480

ggcucgagag ccgggaauac acaaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcccuggcu gccgcugcca uugcguggcu ccuggggucc agcaccucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcccc cgcuuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacgugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcccagga uaagccuacc guggacauug      780

agcuugugac aaccaccguc uccaauaugg cugaagugag aagcuacugc uaugaggccu      840

cuaucuccga uauggccagc gacucccggu gucccacaca aggagaggcu uaccuggaca      900

aacagucuga uacgcaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ucacaugcgc gaaguuugcc ugcuccaaga     1020

aaaugaccgg aaagagcauc cagccugaaa aucucgaaua ccggauuaug cugucugugc     1080

acggcuccca acacagcggg augaucguua acgauacagg acaugagacc gacgagaaua     1140

gagcuaaagu ggaaauuacg ccgaacucuc cccgcgccga ggccacacuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugaccaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc uggggccgau acaggaacac cccauuggaa uaacaaggaa gcgcuggugg     1380

aguuuaaaga cgcucacgcc aagagacaga ccgucguggu ucucggcucu caggaggggg     1440

ccgugcauac ggcucuggcc ggcgcucuug aagccgagau ggauggagcg aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguaccgcc gcuuucacau uuaccaaaau uccggccgaa acacugcaug     1620

gcaccgugac gguugaggug caguacgcug ggaccgacgg accuugcaag guccccgccc     1680

aaauggcggu ggauaugcag acacugaccc cugugggccg ccucaucaca gccaaccccg     1740

uuaucaccga auccacggag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaggcu uuugaagcca cagugagagg agcuaaacgc auggccgucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ucucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcggccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaacc cuccugaugu ggcugggccu caauacaaag aacgggagca     2100

ucucccugau gugucuugcu cugggaggcg uucucauuuu ccugagcacg gccgugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6719
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 2 opt

<400>  6719
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggccu ggcgcucgcc gcugccgcua uugccuggcu gcuugggucc agcacgucuc      600

agaaagucau cuaucuggug augauucucc ugaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uucguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuugagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuggugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccucgaca      900

aacagucuga uacccaguau guuugcaaga gaacacuggu ggaccgcggc uggggcaacg      960

gguguggacu guuuggcaaa gggagccucg ugaccugcgc uaaguucgcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucuggaaua ccggauuaug cuuucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccug ggcggcuuug     1200

ggagccucgg acuggauugu gaaccucgga ccggccuuga cuucuccgau cuguauuacc     1260

ucacaaugaa uaacaagcac uggcuggucc auaaagagug guuucacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccucgugg     1380

aguucaaaga cgcgcacgcu aagagacaga cggucguggu ucugggcucu caggaggggg     1440

ccgugcauac cgcccuugcu ggcgcccugg aagcugagau ggauggagcc aagggccgcc     1500

ucagcuccgg gcaccugaaa ugccggcuua agauggacaa acugagacuc aagggaguca     1560

gcuauucucu guguacagcg gccuuuaccu ucacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccucacac cugugggccg ccugaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcuuga gcuggauccc ccuuuugggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uucgaagcua ccgugagagg agccaagcgc auggcugucc     1920

ucggcgacac cgccugggau uuuggguccg ugggaggcgc ccugaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuca agagccuuuu uggcgggaug uccugguucu     2040

cucaaauccu cauuggaaca cugcugaugu ggcucggccu gaauacgaag aacgggagca     2100

ucucccuuau gugucuggcc cucggaggcg uucugauuuu ucugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6720
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 2 opt

<400>  6720
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg uaucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucauca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6721
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 2 opt

<400>  6721
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaaaucag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ugaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcgcacgcu aagagacaga cgguggucgu gcucggcucu caggaggggg     1440

ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

ugauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc gcuguuucug     2160

ccgacguggg gugcuccguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6722
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 2 opt

<400>  6722
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugccccau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccuggcuc     1680

aaauggccgu ggauaugcag acccucacac ccgugggccg ccugaucacc gcgaacccug     1740

uuaucacgga auccacagag aauagcaaga ugaugcuuga gcuggacccu cccuucgggg     1800

auucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ucggcgacac cgccugggau uucggguccg ugggaggcgc ccugaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuuuu cggcgggaug uccugguuuu     2040

cucaaauccu cauuggaaca cugcugaugu ggcucggccu gaauacgaag aacgggagca     2100

ucucccuuau gugucuggcc cucggaggcg uucugauuuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6723
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 2 opt

<400>  6723
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuac ccuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacaa      360

ccuccacgug ggucguguau ggaaccuguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacacuuccc agccauucca cccgcaagcu gcaaacaaga ucucagaccu      480

ggcucgagag ccgggaauac acgaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacaucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaaccugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcucagga uaagccuacc guggacauug      780

agcuugugac cacaacgguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccaccca aggagaagcc uaccuggaca      900

aacagucuga uacacaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ucacaugcgc uaaguuugcg ugcuccaaga     1020

aaaugaccgg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugugc     1080

acggcuccca acacagcggg augaucguua acgauacggg acaugagaca gacgagaaua     1140

gagccaaagu ggaaauuacc ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga caggccugga cuuuuccgau cuuuauuacc     1260

ugaccaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau acaggaacgc cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga ccgucguggu ucucggcucu caggaggggg     1440

ccgugcauac agcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguaccgcg gccuucacau uuaccaaaau uccggcugaa acgcugcaug     1620

gcaccgugac aguugaggug caguacgccg ggaccgacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acacugaccc cugugggccg ccucaucacg gcgaaccccg     1740

uuaucacaga auccaccgag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac agccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaacg cuccugaugu ggcugggccu caauaccaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcaca gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6724
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 2 opt

<400>  6724
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc guggucucug     2160

cugacguggg gugcuccguu gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6725
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 2 opt

<400>  6725
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccgcuug ggucguguau ggaaccuguc aucacaagaa aggcgaggcc cggcgcucua      420

gacgggcggu uacgcuuccc agccauucca cccgcaagcu gcaaacaaga ccucagaccu      480

ggcucgaguc ucgggaauac acaaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

aucccggcuu cgcccuggcu gccgcugcca uugcguggcu ccuggggagc uccaccagcc      600

agaaagucau cuaucuugug augauucugc ucaucgcccc ugcuuacucu auucggugca      660

ucggaguuuc caaccgcgau uuuguggaag gcaugagcgg gggaacgugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcccagga uaagcccacc guggacauug      780

agcuugugac aaccaccguc ucuaauaugg cugaagugag auccuacugc uaugaggcca      840

gcaucucuga uauggccucc gacagccggu guccuacaca aggagaggcu uaccuggaca      900

aacaguccga uacgcaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggucucugg ucacaugcgc gaaguuugcc ugcagcaaga     1020

aaaugaccgg aaaguccauc cagccggaga aucucgaaua ccggauuaug cugagcgugc     1080

acggcucuca acacuccggg augaucguua acgauacagg acaugagacc gacgagaaua     1140

gagcuaaagu ggaaauuacg cccaacagcc cucgcgccga ggccacacuu ggcggcuucg     1200

ggucucuggg acucgauugu gaaccccgga ccggccugga cuuuagcgau cuuuauuacc     1260

ugaccaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccucugc     1320

ccuggcacgc uggggccgau acaggaaccc cgcauuggaa uaacaaggaa gcgcuggugg     1380

aguuuaaaga cgcucacgcc aagagacaga cagucguggu ucucggcucc caggaggggg     1440

ccgugcauac ggcucuggcc ggcgcucuug aagccgagau ggauggagcg aagggccgcc     1500

ugucuagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

ccuauagccu cuguaccgcc gcuuucacau uuaccaaaau uccugccgaa acacugcaug     1620

gcaccgugac gguugaggug caguacgcug ggaccgacgg acccugcaag gucccugccc     1680

aaauggcggu ggauaugcag acacugaccc ccgugggccg ccucaucaca gccaaccccg     1740

uuaucaccga aucuacggaa aauuccaaga ugaugcugga gcuugacccu ccguucgggg     1800

auagcuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucggucug     1860

gcuccaccau cgggaaggcu uuugaagcca cagugagagg agcuaaacgc auggccgucc     1920

ugggcgacac cgccugggau uucgggagcg ugggaggcgc ucucaacucc cuggggaaag     1980

gcauucacca gaucuucgga gcggccuuua agucucuguu cggcgggaug agcugguuuu     2040

cccaaauccu uauuggaacc cuccugaugu ggcugggccu caauacaaag aacgggucua     2100

ucagccugau gugucuugcu cugggaggcg uucucauuuu ccuguccacg gccgugagcg     2160

ccgacgucgg gugcucugug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6726
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 2 opt

<400>  6726
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucua      600

aaaaggucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaaaccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca gggagaagcc uaccuggaca      900

agcaaucuga uacccaguau guuugcaaaa gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaag gggagccugg ugaccugcgc uaaguuugcg ugcuccaaaa     1020

agaugacagg aaaaagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca gcacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaggu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaaacac uggcucgucc auaaggagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaagaa gcccuggugg     1380

aguuuaagga cgcgcacgcu aagagacaaa cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aaaggccgcc     1500

ugagcuccgg gcaccucaag ugccggcuga aaauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaagau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

agauggccgu ggauaugcaa acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaaa ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaaa agaaaauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaggcc uuugaggcua ccgugagagg agccaaacgc auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaagg     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucagauccu uauuggaaca cuccugaugu ggcugggccu caauacgaaa aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6727
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 2 opt

<400>  6727
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgcggc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucu auucgcugca      660

ucggaguuuc caacagagau uuuguggaag gcaugagcgg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc ucuaauaugg ccgaagugcg guccuacugc uaugaggcua      840

gcaucucuga uauggccucc gacagcagau gucccacgca aggagaagcc uaccuggaca      900

aacaguccga uacccaguau guuugcaagc gcacacucgu ggaccggggc uggggcaacg      960

gguguggacu guucggcaaa gggucucugg ugaccugcgc uaaguuugcg ugcagcaaga     1020

aaaugacagg aaaguccauc cagccugaga aucucgaaua cagaauuaug cugagcgucc     1080

acggcucuca acacuccggg augaucguua acgauaccgg acaugagacg gacgagaauc     1140

gcgccaaagu ggaaauuaca ccgaacagcc cccgggcuga ggccacccuu ggcggcuucg     1200

ggucucuggg acucgauugu gaaccuagaa ccggccugga cuuuagcgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagcgccaga cggucguggu ucucggcucc caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccggc     1500

ugucuagcgg gcaccucaaa ugcagacuga agauggacaa acuucgccug aagggagucu     1560

ccuauagccu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg gcucaucacc gcgaaccccg     1740

uuaucacgga aucuacagaa aauuccaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acagcuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg cauagaucug     1860

gcuccacaau cgggaaagcc uuugaggcua ccgugcgcgg agccaagcgg auggcugucc     1920

ugggcgacac cgccugggau uucgggagcg ugggaggcgc ccucaacucc cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agucucuguu cggcgggaug agcugguuuu     2040

cccaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggucua     2100

ucagccugau gugucuugcc cugggaggcg uucucauuuu ccuguccacc gcugugagcg     2160

ccgacgucgg gugcucugug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6728
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 2 opt

<400>  6728
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaagcgcuga caccuccgug ggcaucgucg      120

ggcugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggaucu gccuacuaua      180

uguaccuuga uagaaaugac gccggcgagg cuauuagcuu ccccacaacc cuggggauga      240

acaaaugcua uauccagauu auggaucucg gacacaugug ugacgccaca auguccuacg      300

aaugcccuau gcuggaugag ggcguggaac cggacgaugu ggacuguugg ugcaauacca      360

caucuaccug ggucguguau gggacguguc aucacaagaa aggagaggcu cggcgcagca      420

gacgggccgu uacccuuccc ucccauucua cacgcaagcu gcaaaccaga agccagacau      480

ggcucgaguc ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccugggcagc ucuacguccc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacagc auucggugca      660

ucgggguuuc uaaccgcgau uuuguggaag gaauguccgg cgggacaugg gucgacgugg      720

ugcuggagca uggaggcugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc agcaauaugg ccgaagugag aucuuacugc uaugaggcuu      840

ccaucagcga uauggccucc gacucucggu gucccacgca aggggaagcc uaccuggaca      900

aacagagcga uacccaguau guuugcaaga gaacacucgu ggaccgcgga uggggcaacg      960

gcugugggcu guucggaaaa ggcucccugg ugaccugcgc uaaguuugcg ugcagcaaga     1020

aaaugacagg gaagucuauc cagccugaga aucucgaaua ccggauuaug cuguccgugc     1080

acggaagcca acacucuggc augaucguua acgauaccgg gcaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacagcc cccgcgcuga ggccacccuu ggaggcuucg     1200

gcucccuggg gcucgauugu gaaccucgga ccggacugga cuuuucugau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggcgcugau accgggacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggaagc caggagggcg     1440

ccgugcauac cgcccuggcu ggggcccuug aagcugagau ggauggcgcc aagggacgcc     1500

uguccagcgg ccaccucaaa ugccggcuga agauggacaa acuuagacug aagggggucu     1560

cuuauucccu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gaacggugac cguugaggug caguacgccg gcacagacgg gccuugcaag guccccgcuc     1680

aaauggccgu ggauacccag acacugaccc cugugggacg ccucaucacg gcgaaccccg     1740

uuaucacaga aagcaccgaa aauucuaaga ugaugcugga gcuugauccc ccuuucggcg     1800

acuccuacau ugucaucggg gugggcgaga aaaagauuac acaccacugg caucggagcg     1860

gauccaccau cggcaaagcc uuugaggcua ccgugagagg ggccaagcgc auggcugucc     1920

ugggagacac agccugggau uucggcucug ucgggggagc ccucaacagc cugggcaaag     1980

ggauucacca gaucuucggc gcugcguuua agucccuguu cggaggcaug ucuugguuua     2040

gccaaauccu uauugggacg cuccugaugu ggcugggacu caauaccaag aacggcucca     2100

ucagccugau gugucuugcc cucgggggag uucugauuuu ccugucuaca gcuguguccg     2160

ccgacgucgg cugcagcgug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6729
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 2 opt

<400>  6729
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccaaugug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcagc gccuacuaua      180

uguaccuuga uagaaacgac gccggggagg cuauuuccuu ccccacaacc cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augucuuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaacacca      360

caagcaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucca      420

gacgggccgu uacccuuccc ucucauagca cacgcaagcu gcaaaccaga ucccagacau      480

ggcucgaguc ucgggaauac accaagcacc ugauccgcgu ggagaauugg aucuuuagaa      540

acccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggagc uccacgagcc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucu auucggugca      660

ucggaguuuc caaucgcgau uuuguggaag gcaugagcgg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc ucuaacaugg ccgaagugag auccuacugc uaugaggcua      840

gcaucucuga uauggccucc gacagccggu gucccacgca aggagaagcc uaccuggaca      900

aacaguccga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucucugg ugaccugcgc uaaguuugcg ugcagcaaga     1020

aaaugacagg aaaguccauc cagccugaga accucgaaua ccggauuaug cugagcgucc     1080

acggcucuca acacuccggg augaucguua augauaccgg acaugagacg gacgagaaca     1140

gagccaaagu ggaaauuaca ccgaauagcc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggucucuggg acucgauugu gaaccucgga ccggccugga cuuuagcgau cuuuauuacc     1260

ugacaaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa caacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucc caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugucuagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

ccuauagccu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaucccg     1740

uuaucacgga aucuacagag aacuccaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acagcuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucggucug     1860

gcuccacaau cgggaaagcc uuugaagcua ccgugagagg agcccgccgg auggcugucc     1920

ugggcgacac cgccugggau uucgggagcg ugggaggcgc ccucaauucc cuggggaagg     1980

gcauucacca gaucuucgga gcugcguuua aaucucuguu cggcgggaug agcugguuuu     2040

cccaaauccu uauuggaaca cuccugaugu ggcugggccu caacacgaag aaugggucua     2100

ucagccugau gugucuugcc cugggaggcg uucucauuuu ccuguccacc gcugugagcg     2160

ccgacgucgg gugcucugug gauuuuucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6730
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 2 opt

<400>  6730
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga aaccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug agguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga cagaaaugau gccggggaag cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaccucg gccacaugug ugaugccaca augagcuacg      300

agugcccuau gcuggacgaa gggguggagc cggaugacgu ggauuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgaaag ccgggaguac accaagcacc ugauccgcgu ggaaaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgac uuuguggagg gcaugucugg gggaacaugg gucgaugugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga caagccuaca guggauauug      780

aacuugugac caccacaguc uccaauaugg ccgaggugag aagcuacugc uaugaggcuu      840

cuaucuccga cauggccagc gauucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga cacccaguau guuugcaaga gaacacucgu ggaucgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaaa aucucgagua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgacaccgg acaugagacg gaugaaaaua     1140

gagccaaagu ggagauuaca ccgaacucuc cccgcgcuga agccacccuu ggcggcuucg     1200

ggagccuggg acucgacugu gagccucgga ccggccugga uuuuuccgac cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagaaug guuccacgau aucccccugc     1320

cuuggcacgc cggggcugac accggaacac cccauuggaa uaacaaggag gcccuggugg     1380

aguuuaaaga ugcgcacgcu aagagacaga cggucguggu ucucggcucu caggaagggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aggcugaaau ggacggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggauaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugag acccugcaug     1620

gcacggugac cguugaagug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggacaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga guccacagag aauagcaaga ugaugcugga acuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgauac cgccugggac uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgaugucgg gugcuccgug gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6731
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 2 opt

<400>  6731
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc cugcggugca      660

uuggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuugagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacaucg      780

agcuggugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuauuuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccucgaca      900

aacagucuga uacccaguau guuugcaaga gaacacuggu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccucg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucuggaaua ccggaucaug cuuucugucc     1080

acggcuccca acacagcggg augauuguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaaucaca ccgaacucuc cccgcgcuga ggccacccug ggcggcuucg     1200

ggagccucgg acuggauugu gaaccucgga ccggccuuga cuuuuccgau cuguauuacc     1260

ucacaaugaa uaacaagcac uggcuggucc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccucgugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucugggcucu caggaggggg     1440

ccgugcauac cgcccuugcu ggcgcccugg aagcugagau ggauggagcc aagggccgcc     1500

ucagcuccgg gcaccugaaa ugccggcuua agauggacaa acugagacuc aagggaguca     1560

gcuauucucu guguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccucacac cugugggccg ccugauuacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcuuga gcuggauccc ccuuucgggg     1800

acucuuacau cgucauuggc gugggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ucggcgacac cgccugggau uucggguccg ugggaggcgc ccugaacucu cuggggaaag     1980

gcauccacca gauuuucgga gcugcguuua agagccuuuu cggcgggaug uccugguuuu     2040

cucaaauccu caucggaaca cugcugaugu ggcucggccu gaauacgaag aacgggagca     2100

uuucccuuau gugucuggcc cucggaggcg uucugaucuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6732
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 2 opt

<400>  6732
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccaaugug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcagc gccuacuaua      180

uguaccuuga uagaaacgac gccggggagg cuauuuccuu ccccacaacc cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augucuuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaacacca      360

caagcaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucca      420

gacgggccgu uacccuuccc ucucauagca cacgcaagcu gcaaaccaga ucccagacau      480

ggcucgaguc ucgggaauac accaagcacc ugauccgcgu ggagaauugg auguuuagaa      540

acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggagc uccacgagcc      600

agaaagucau uuaucuugug augauccugc ucauugcgcc cgccuacucu auccggugca      660

uuggaguuuc caaucgcgau uuuguggaag gcaugagcgg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacaucg      780

agcuugugac caccacaguc ucuaacaugg ccgaagugag auccuacugc uaugaggcua      840

gcauuucuga uauggccucc gacagccggu gucccacgca aggagaagcc uaccuggaca      900

aacaguccga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucucugg ugaccugcgc uaaguuugcg ugcagcaaga     1020

aaaugacagg aaaguccauc cagccugaga accucgaaua ccggaucaug cugagcgucc     1080

acggcucuca acacuccggg augauuguua augauaccgg acaugagacg gacgagaaca     1140

gagccaaagu ggaaaucaca ccgaauagcc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggucucuggg acucgauugu gaaccucgga ccggccugga cuuuagcgau cuuuauuacc     1260

ugacaaugaa caauaagcac uggcucgucc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa caacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucc caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugucuagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

ccuauagccu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucauuacc gcgaaucccg     1740

uuaucacgga aucuacagag aacuccaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acagcuacau cgucauuggc gugggagaga aaaagaucac ccaccacugg caucggucug     1860

gcuccacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucgggagcg ugggaggcgc ccucaauucc cuggggaaag     1980

gcauccacca gauuuucgga gcugcguuua agucucuguu cggcgggaug agcugguuuu     2040

cccaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag aaugggucua     2100

uuagccugau gugucuugcc cugggaggcg uucucaucuu ccuguccacc gcugugagcg     2160

ccgacgucgg gugcucugug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6733
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 2 opt

<400>  6733
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg auccugagaa      540

auccuggcuu ugcgcucgcc gcugccgcua uugccuggcu gcuugggucc agcacgucuc      600

agaaagucau cuaucuggug augauucucc ugaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uucguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuugagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuggugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccucgaca      900

aacagucuga uacccaguau guuugcaaga gaacacuggu ggaccgcggc uggggcaacg      960

gguguggacu guuuggcaaa gggagccucg ugaccugcgc uaaguucgcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucuggaaua ccggauuaug cuuucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccug ggcggcuuug     1200

ggagccucgg acuggauugu gaaccucgga ccggccuuga cuucuccgau cuguauuacc     1260

ucacaaugaa uaacaagcac uggcuggucc auaaagagug guuucacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccucgugg     1380

aguucaaaga cgcgcacgcu aagagacaga cggucguggu ucugggcucu caggaggggg     1440

ccgugcauac cgcccuugcu ggcgcccugg aagcugagau ggauggagcc aagggccgcc     1500

ucagcuccgg gcaccugaaa ugccggcuua agauggacaa acugagacuc aagggaguca     1560

gcuauucucu guguacagcg gccuuuaccu ucacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccucacac cugugggccg ccugaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcuuga gcuggauccc ccuuuugggg     1800

acucuuacau ugucaucggc auuggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uucgaagcua ccgugagagg agccaagcgc auggcugugc     1920

ucggcgacac cgccugggau uuuggguccg ucggaggcgc ccugaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuca agagccuuuu uggcgggaug uccugguucu     2040

cucaaauucu caucggaaca cugcugaugu ggcucggccu gaauacgaag aacgggagca     2100

uuucccuuau gugucuggcc cucggaggcg ugcugaucuu ucugagcacc gcuguuucug     2160

ccgacguggg gugcuccguc gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6734
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 2 opt

<400>  6734
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuuguguc caccaccguc agcaauaugg ccgaagugag aucuuacugc uaugaggcua      840

caaucuccga uaucgccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggccgucgu gcucggcucu caggaggggg     1440

ccguucauac cgcucuggcc ggcgcucuug aagccgagau ggauggagcg aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcc gcuuucaccu uuacaaaaau cccggccgaa acccugcaug     1620

gcacggucac cguggagguu caguacgcug ggacagacgg accuugcaag gugcccgccc     1680

aaauggcggu cgauaugcag acccugacac cugugggccg ccucaucacc gccaaccccg     1740

ugauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaggcu uuugaagcca ccgugagagg agcuaaacgc auggccgugc     1920

ugggcgacac cgccugggau uucggguccg ucggaggcgc ucucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcggccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcu cugggaggcg ugcucaucuu ccugagcacc gccguuucug     2160

ccgacguggg gugcuccguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6735
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 2 opt

<400>  6735
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcacc caggaggggg     1440

ccgugcauac agcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugucuagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

ccuauagccu cuguaccgcg gccuucacau uuaccaaaau uccggcugaa acgcugcaug     1620

gcaccgugac aguugaggug caguacgccg ggaccgacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acacugaccc cugugggccg ccucaucacg gcgaaccccg     1740

uuaucacaga aucuaccgaa aauuccaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acagcuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucggucug     1860

gcuccaccau cgggaaagcc uuugaggcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac agccugggau uucgggagcg ugggaggcgc ccucaacucc cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agucucuguu cggcgggaug agcugguuuu     2040

cccaaauccu uauuggaacg cuccugaugu ggcugggccu caauaccaag aacgggucua     2100

ucagccugau gugucuugcc cugggaggcg uucucauuuu ccuguccaca gcugugagcg     2160

ccgacgucgg gugcucugug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6736
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 2 opt

<400>  6736
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugagcug caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccucacac cugugggccg ccugaucacc gcgaaccccg     1740

ugaucacgga auccacagag aauagcaaga ugaugcuuga gcuggauccc ccuuucgggg     1800

acucuuacau uguuaucggc gucggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ucggcgacac cgccugggau uucggguccg ucggaggcgc ccugaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuuuu cggcgggaug uccugguuuu     2040

cucaaauccu cauuggaaca cugcugaugu ggcucggccu gaauacgaag aacgggagca     2100

ucucccuuau gugucuggcc cucggaggcg ugcugauuuu ccugagcacc gcuguuucug     2160

ccgacguggg gugcuccguc gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6737
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 2 opt

<400>  6737
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga aaccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug agguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga cagaaaugau gccggggaag cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaccucg gccacaugug ugaugccaca augagcuacg      300

agugcccuau gcuggacgaa gggguggagc cggaugacgu ggauuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgaaag ccgggaguac accaagcacc ugauccgcau cgaaaacugg auuuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc agcacgucuc      600

agaaagugau uuaucuuguc augauccugc ucauugcgcc cgccuacucc auccggugca      660

uuggagugag caaccgcgac uuuguugagg gcaugucugg gggaacaugg guggaugucg      720

ugcuggagca uggcgggugu gugaccguca uggcucagga caagccuaca guugauaucg      780

aacuugugac caccacagug uccaauaugg ccgaggucag aagcuacugc uaugaggcuu      840

cuaucuccga cauggccagc gauucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga cacccaguau gugugcaaga gaacacucgu ugaucgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaaa aucucgagua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgacaccgg acaugagacg gaugaaaaua     1140

gagccaaagu ugagaucaca ccgaacucuc cccgcgcuga agccacccuu ggcggcuucg     1200

ggagccuggg acucgacugu gagccucgga ccggccugga uuuuuccgac cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagaaug guuccacgau auuccccugc     1320

cuuggcacgc cggggcugac accggaacac cccauuggaa uaacaaggag gcccuggucg     1380

aguuuaaaga ugcgcacgcu aagagacaga cgguggucgu gcucggcucu caggaagggg     1440

ccguucauac cgcccuggcu ggcgcccuug aggcugaaau ggacggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggauaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugag acccugcaug     1620

gcacggucac cguggaaguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgacaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

ugauuacgga guccacagag aauagcaaga ugaugcugga acuugauccc ccuuucgggg     1800

acucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgauac cgccugggac uucggguccg ucggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc gcuguuucug     2160

ccgauguggg gugcuccguc gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6738
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 2 opt

<400>  6738
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga auccauugag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cgucauuggc gugggagaga aaaagaucac acaccacugg caucgguccg     1860

gcagcaccau ugggaaagcc uuugaagcua cagugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaacc cuccugaugu ggcugggccu caauacaaag aacgggagca     2100

uuucccugau gugucuugcc cugggaggcg uucucaucuu ccugagcacg gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6739
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 2 opt

<400>  6739
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggaguc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcc gcguucaccu uuacaaaaau uccggccgaa acccugcaug     1620

gcacgguuac cguggagguc caguacgcug ggacagacgg accuugcaag gugcccgccc     1680

aaauggcugu ggauaugcag acccugacac cuguuggccg ccucaucacc gccaaccccg     1740

ucaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugugaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcg uuugaagcca ccgucagagg agcuaagcgc auggccgugc     1920

ugggcgacac cgcuugggau uucggguccg uuggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gccgcuuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucuccuucau gugucuggcg cuuggaggcg ugcugauuuu ucucagcacc gccgucucug     2160

cugacguggg gugcuccguu gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6740
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 2 opt

<400>  6740
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggcaaac     1500

ugagcuccgg gcaccucaag ugccgccuga aaauggacaa acuucggcug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaagau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggcag acucaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaaa ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaaa agaaaauuac ccaccacugg caucgcuccg     1860

gcagcacaau cgggaaggcc uuugaggcua ccgugcgggg agccaaaaga auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaagg     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaaa aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6741
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 2 opt

<400>  6741
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga ggcgcacgcu aagagacaga cggucguggu ucucggcucu caggaagggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aggcugaaau ggacggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggauaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugag acccugcaug     1620

gcacggugac cguugaagug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggacaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga guccacagag aauagcaaga ugaugcugga acuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgauac cgccugggac uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgaugucgg gugcuccgug gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6742
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 2 opt

<400>  6742
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg gggcugacgg accuugcaag guccccgccc     1680

aaauggcggu ggauaugcag acacugaccc cugugggccg ccucaucaca gccaaccccg     1740

uuaucaccga auccacggag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaggcu uuugaagcca cagugagagg agcuaaacgc auggccgucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ucucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcggccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaacc cuccugaugu ggcugggccu caauacaaag aacgggagca     2100

ucucccugau gugucuugcu cugggaggcg uucucauuuu ccugagcacg gccgugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6743
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 2 opt

<400>  6743
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc cggcaaggag aaaaagcggc gcggggcuga caccaaugug ggaaucgucg      120

gccugcuccu gacaacggcc auggcggcug aaguuaccag acgggggagc gccuacuaua      180

uguaccuuga ucgcaacgac gccggagagg cuauuuccuu ccccacaacc cugggcauga      240

auaaaugcua uauccagauu auggaucucg ggcacaugug ugacgccaca augucuuacg      300

aaugcccuau gcuggaugag ggaguggaac cggacgaugu ggacuguugg ugcaacacca      360

caagcaccug ggucguguau ggcacguguc aucacaagaa aggcgaggcu agacgguccc      420

gcagagccgu uacccuuccc ucucauagca cacggaagcu gcaaacccgc ucccagacau      480

ggcucgaguc uagagaauac accaagcacc ugauccgggu ggagaauugg aucuuucgca      540

acccuggguu cgcgcuggcc gcugccgcua uugccuggcu ccugggaagc uccacgagcc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucu auuagaugca      660

ucggcguuuc caaucgggau uuuguggaag ggaugagcgg aggcacaugg gucgacgugg      720

ugcuggagca ugggggaugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc ucuaacaugg ccgaagugcg cuccuacugc uaugaggcua      840

gcaucucuga uauggccucc gacagcagau gucccacgca aggcgaagcc uaccuggaca      900

aacaguccga uacccaguau guuugcaagc ggacacucgu ggacagaggc ugggggaaug      960

gauguggccu guucgggaaa ggaucucugg ugaccugcgc uaaguuugcg ugcagcaaga     1020

aaaugacagg caaguccauc cagccugaga accucgaaua ccgcauuaug cugagcgucc     1080

acgggucuca acacuccgga augaucguua augauaccgg ccaugagacg gacgagaacc     1140

gggccaaagu ggaaauuaca ccgaauagcc ccagagcuga ggccacccuu ggcggguucg     1200

gaucucuggg ccucgauugu gaaccucgca ccgggcugga cuuuagcgau cuuuauuacc     1260

ugacaaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggagcugau accggcacac cccauuggaa caacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagcggcaga cggucguggu ucucgggucc caggagggcg     1440

ccgugcauac cgcccuggcu ggagcccuug aagcugagau ggauggcgcc aaggggagac     1500

ugucuagcgg acaccucaaa ugccgccuga agauggacaa acuucggcug aagggcgucu     1560

ccuauagccu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

ggacggugac cguugaggug caguacgccg gaacagacgg cccuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cuguggggag acucaucacc gcgaaucccg     1740

uuaucacgga aucuacagag aacuccaaga ugaugcugga gcuugauccc ccuuucggcg     1800

acagcuacau ugucaucgga gugggcgaga aaaagauuac ccaccacugg caucgcucug     1860

gguccacaau cggaaaagcc uuugaagcua ccgugcgggg cgccagacgc auggcugucc     1920

ugggggacac cgccugggau uucggaagcg ugggcggggc ccucaauucc cugggcaagg     1980

gcauucacca gaucuucgga gcugcguuua aaucucuguu cgggggaaug agcugguuuu     2040

cccaaauccu uauuggcaca cuccugaugu ggcuggggcu caacacgaag aauggaucua     2100

ucagccugau gugucuugcc cugggcgggg uucucauuuu ccuguccacc gcugugagcg     2160

ccgacgucgg cugcucugug gauuuuucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6744
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 2 opt

<400>  6744
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga aaccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug agguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga cagaaaagau gccggggaag cuauuucuuu ccccacaacc cugggaauga      240

auaagugcua uauccagauu auggaccucg gccacaugug ugaugccaca augagcuacg      300

agugcccuau gcuggacgaa gggguggagc cggaugacgu ggauuguugg ugcaacacca      360

cauccaccug ggucguguau ggaacguguc aucacaaaaa gggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgaaag ccgggaguac accaaacacc ugauccgcgu ggaaaauugg aucuuuagaa      540

acccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaggucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaucgcgac uuuguggagg gcaugucugg gggaacaugg gucgaugugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga caaaccuaca guggauauug      780

aacuugugac caccacaguc uccaacaugg ccgaggugag aagcuacugc uaugaggcuu      840

cuaucuccga cauggccagc gauucccggu gucccacgca aggagaagcc uaccuggaca      900

agcagucuga cacccaguau guuugcaaaa gaacacucgu ggaucgcggc uggggcaaug      960

gguguggacu guucggcaag gggagccugg ugaccugcgc uaaguuugcg ugcuccaaaa     1020

agaugacagg aaaaagcauc cagccugaaa accucgagua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua augacaccgg acaugagacg gaugaaaaca     1140

gagccaaggu ggagauuaca cccaauucuc cgcgcgcuga agccacccuu ggcggcuucg     1200

ggagccuggg acucgacugu gagccucgga ccggccugga uuuuuccgac cuuuauuacc     1260

ugacaaugaa caauaaacac uggcucgucc auaaggaaug guuccacgau aucccccugc     1320

cuuggcacgc cggggcugac accggaacac cccauuggaa caauaaagag gcccuggugg     1380

aguuuaagga ugcgcacgcu aagagacaga cggucguggu ucucggcucu caggaagggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aggcugaaau ggacggagcc aaaggccgcc     1500

ugagcuccgg gcaccucaag ugccggcuga aaauggauaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaagau uccggcugag acccugcaug     1620

gcacggugac cguugaagug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggacaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga guccacagag aacagcaaaa ugaugcugga acuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga agaaaauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaggcc uuugaagcua ccgugagagg agccaaacgc auggcugucc     1920

ugggcgauac cgccugggac uucggguccg ugggaggcgc ccucaauucu cuggggaagg     1980

gcauucacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caacacgaaa aaugggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgaugucgg gugcuccgug gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6745
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 2 opt

<400>  6745
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uaugguuagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu cgaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc caaguuugcu ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucuguuc     1080

acggcuccca acacagcggg augaucguga acgauaccgg acaugagacg gacgagaaua     1140

gagcgaaagu cgaaauuaca ccgaacucuc cccgcgccga ggcuacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggccgau accggaacac cccauuggaa uaacaaggaa gcucuggucg     1380

aguuuaaaga cgcccacgcg aagagacaga cggugguugu gcucggcucu caggaggggg     1440

cuguccauac cgcccuggcc ggcgcucuug aagccgagau ggauggagcu aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguga     1560

gcuauucucu cuguacagcc gcguucaccu uuacaaaaau uccggccgaa acccugcaug     1620

gcacgguuac cguggagguc caguacgcug ggacagacgg accuugcaag gugcccgccc     1680

aaauggcugu ggauaugcag acccugacac cuguuggccg ccucaucacc gccaaccccg     1740

ucaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugugaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcg uuugaagcca ccgucagagg agcuaagcgc auggccgugc     1920

ugggcgacac cgcuugggau uucggguccg uuggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gccgcuuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcg cugggaggcg ugcucauuuu ccugagcacc gccgucucug     2160

cugacguggg gugcuccguu gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6746
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 2 opt

<400>  6746
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccaaugug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcagc gccuacuaua      180

uguaccuuga uagaaacgac gccggggagg cuauuuccuu ccccacaacc cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augucuuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaacauga      360

ccagcacaug ggucguguau ggaaccuguc aucacaagaa aggcgaggcu cggcgcucca      420

gacgggccgu uacgcuuccc ucucauagca cccgcaagcu gcaaacaaga ucccagaccu      480

ggcucgaguc ucgggaauac acaaagcacc ugauccgcgu ggagaauugg aucuuuagaa      540

acccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggagc uccaccagcc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucu auucggugca      660

ucggaguuuc caaucgcgau uuuguggaag gcaugagcgg gggaacgugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcucagga uaagccuacc guggacauug      780

agcuugugac aaccaccguc ucuaacaugg ccgaagugag auccuacugc uaugaggcua      840

gcaucucuga uauggccucc gacagccggu gucccacaca aggagaagcc uaccuggaca      900

aacaguccga uacgcaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucucugg ucacaugcgc uaaguuugcg ugcagcaaga     1020

aaaugaccgg aaaguccauc cagccugaga accucgaaua ccggauuaug cugagcgugc     1080

acggcucuca acacuccggg augaucguua augauacagg acaugagacc gacgagaaca     1140

gagccaaagu ggaaauuacg ccgaauagcc cccgcgcuga ggccacacuu ggcggcuucg     1200

ggucucuggg acucgauugu gaaccucgga ccggccugga cuuuagcgau cuuuauuacc     1260

ugaccaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau acaggaacac cccauuggaa caacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga ccgucguggu ucucggcucc caggaggggg     1440

ccgugcauac ggcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugucuagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

ccuauagccu cuguaccgcg gccuucacau uuaccaaaau uccggcugaa acacugcaug     1620

gcaccgugac gguugaggug caguacgccg ggaccgacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acacugaccc cugugggccg ccucaucaca gcgaaucccg     1740

uuaucaccga aucuacggag aacuccaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acagcuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucggucug     1860

gcuccaccau cgggaaagcc uuugaagcua cagugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucgggagcg ugggaggcgc ccucaauucc cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agucucuguu cggcgggaug agcugguuuu     2040

cccaaauccu uauuggaacc cuccugaugu ggcugggccu caacacaaag aaugggucua     2100

ucagccugau gugucuugcc cugggaggcg uucucauuuu ccuguccacg gcugugagcg     2160

ccgacgucgg gugcucugug gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6747
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 2 opt

<400>  6747
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaga aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augaucguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaauuaca ccgaacucuc cccgcgcuga ggccgcccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugaccaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc uggggccgau acaggaacac cccauuggaa uaacaaggaa gcgcuggugg     1380

aguuuaaaga cgcucacgcc aagagacaga ccgucguggu ucucggcucu caggaggggg     1440

ccgugcauac ggcucuggcc ggcgcucuug aagccgagau ggauggagcg aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguaccgcc gcuuucacau uuaccaaaau uccggccgaa acacugcaug     1620

gcaccgugac gguugaggug caguacgcug ggaccgacgg accuugcaag guccccgccc     1680

aaauggcggu ggauaugcag acacugaccc cugugggccg ccucaucaca gccaaccccg     1740

uuaucaccga auccacggag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaggcu uuugaagcca cagugagagg agcuaaacgc auggccgucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ucucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcggccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaacccu uaucggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

uuucccugau gugucuugcu cugggaggcg uucucaucuu ccugagcacc gccgugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6748
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 2 opt

<400>  6748
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga aaccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug agguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga cagaaaugau gccggggaag cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaccucg gccacaugug ugaugccaca augagcuacg      300

agugccccau gcuggacgaa gggguggagc cggaugacgu ggauuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgaaag ccgggaguac accaagcacc ugauccgcgu ggaaaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcu ggccuacucc auucggugca      660

ucggaguuag caaccgcgac uuuguggagg gcaugucugg gggaacaugg gucgaugugg      720

ugcuugagca uggcgggugu gucaccguua uggcucagga caagcccaca guggauauug      780

aacuggugac caccacaguc uccaauaugg ccgaggugag aagcuacugc uaugaggcuu      840

cuaucuccga cauggccagc gauucccggu guccuacgca aggagaagcc uaccucgaca      900

aacagucuga cacccaguau guuugcaaga gaacacuggu ggaucgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccucg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc cagcccgaaa aucuggagua ccggauuaug cuuucugucc     1080

acggcuccca acacagcggg augaucguua acgacaccgg acaugagacg gaugaaaaua     1140

gagccaaagu ggagauuaca ccuaacucuc cgcgcgcuga agccacccug ggcggcuucg     1200

ggagccucgg acuggacugu gagccccgga ccggccuuga uuuuuccgac cuguauuacc     1260

ucacaaugaa uaacaagcac uggcuggucc auaaagaaug guuccacgau aucccucugc     1320

ccuggcacgc cggggcugac accggaacac cucauuggaa uaacaaggag gcccucgugg     1380

aguuuaaaga ugcgcacgcu aagagacaga cggucguggu ucugggcucu caggaagggg     1440

ccgugcauac cgcccuugcu ggcgcccugg aggcugaaau ggacggagcc aagggccgcc     1500

ucagcuccgg gcaccugaaa ugccggcuua agauggauaa acugagacuc aagggaguca     1560

gcuauucucu guguacagcg gccuucaccu uuacaaaaau ucccgcugag acccugcaug     1620

gcacggugac cguugaagug caguacgccg ggacagacgg accgugcaag gucccugcuc     1680

aaauggccgu ggacaugcag acccucacac ccgugggccg ccugaucacc gcgaacccug     1740

uuaucacgga guccacagag aauagcaaga ugaugcuuga acuggauccu cccuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ucggcgauac cgccugggac uucggguccg ugggaggcgc ccugaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agagccuuuu cggcgggaug uccugguuuu     2040

cucaaauccu cauuggaaca cugcugaugu ggcucggccu gaauacgaag aacgggagca     2100

ucucccuuau gugucuggcc cucggaggcg uucugauuuu ccugagcacc gcugugucug     2160

ccgaugucgg gugcuccgug gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6749
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 2 opt

<400>  6749
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaauuag caaccgcgau uuuguugaag gcaugucugg gggaacaugg guggacgucg      720

ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca guugacaucg      780

agcuugugac caccacagug uccaauaugg ccgaagucag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguga acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ugaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggauucuggg acucgauugu gaaccucgga ccggccugga cuuuagcgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgugc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcgcacgcu aagagacaga cgguggucgu gcucggcucc caggaggggg     1440

ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugucuagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagugu     1560

ccuauagccu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu cgauaugcag acccugacac cugugggccg ccucauuacc gcgaaccccg     1740

ugaucacgga aucuacagaa aauuccaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acagcuacau uguuaucggc gucggagaga aaaagauuac ccaccacugg caucggucug     1860

gcuccacaau cgggaaagcc uuugaggcua ccgugagagg agccaagcgc auggcugugc     1920

ugggcgacac cgccugggau uucgggagcg ucggaggcgc ccucaacucc cuggggaaag     1980

gcauccacca gauuuucgga gcugcguuua agucucuguu cggcgggaug agcugguuuu     2040

cccaaauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggucua     2100

ucagccugau gugucuugcc cugggaggcg ugcucauuuu ccuguccacc gcuguuagcg     2160

ccgacguggg gugcucuguc gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6750
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 2 opt

<400>  6750
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacacaug ugacgccacc augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacaa      360

ccuccacgug ggucguguau ggaaccuguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacacuuccc agccauucca uccgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg auuuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc agcacgucuc      600

agaaagucau uuaucuugug augauccugc ucauugcgcc cgccuacucc auccggugca      660

uuggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacaucg      780

agcuugugac caccacaguc uccaauaugg ccgaagugag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauu cagccugaga aucucgaaua ccggaucaug cugucugucc     1080

acggcuccca acacagcggg augauuguua acgauaccgg acaugagacg gacgagaaua     1140

gagccaaagu ggaaaucaca ccgaacucuc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuauuacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau cgucauuggc gugggagaga aaaagaucac ccaccacugg caucgguccg     1860

gcagcacaau ugggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauccacca gaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu uaucggaaca cuccugaugu ggcugggccu caaugccaag aacgggagca     2100

uuucccugau gugucuugcu cugggaggcg uucucaucuu ccugagcacg gccgugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6751
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 2 opt

<400>  6751
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu caacggucuc      420

gcagagccgu uacccuuccc agccauucca cacggaagcu gcagacccgc ucucagacau      480

ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaacugg aucuuucgca      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auuagaugca      660

ucggaguuag caaccgggau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucaaga uaagccuaca guggacauug      780

agcuugugac caccacaguc uccaauaugg ccgaagugcg cagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacuccagau gucccacgca gggagaagcc uaccuggaca      900

aacagucuga uacccaguau guuugcaagc ggacacucgu ggacagaggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg ugcuccaaga     1020

aaaugacagg aaagagcauc caaccugaga aucucgaaua ccgcauuaug cugucugucc     1080

acggcuccca gcacagcggg augaucguua acgauaccgg acaugagacg gacgagaauc     1140

gggccaaagu ggaaauuaca ccgaacucuc ccagagcuga ggccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau cuuuauuacc     1260

ugacaaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa uaacaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagcggcaga cggucguggu ucucggcucu caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggcagac     1500

ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug aagggaguca     1560

gcuauucucu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caauacgccg ggacagacgg accuugcaag guccccgcuc     1680

agauggccgu ggauaugcag acccugacac cugugggcag acucaucacc gcgaaccccg     1740

uuaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucgcuccg     1860

gcagcacaau cgggaaagcc uuugaagcua ccgugcgggg agccaagaga auggcugucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ccucaacucu cuggggaaag     1980

gcauucacca aaucuucgga gcugcguuua agagccuguu cggcgggaug uccugguuuu     2040

cucagauccu uauuggaaca cuccugaugu ggcugggccu caauacgaag aacgggagca     2100

ucucccugau gugucuugcc cugggaggcg uucucauuuu ccugagcacc gcugugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6752
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 2 opt

<400>  6752
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaagcgaga ucucagaccu      480

ggcucgagag ccgggaauac acaaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcccuggcu gccgcugcca uugcguggcu ccuggggucc agcaccucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcccc cgcuuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacgugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcccagga uaagccuacc guggacauug      780

agcuugugac aaccaccguc uccaauaugg cugaagugag aagcuacugc uaugaggccu      840

cuaucuccga uauggccagc gacucccggu gucccacaca aggagaggcu uaccuggaca      900

aacagucuga uacgcaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ucacaugcgc gaaguuugcc ugcuccaaga     1020

aaaugaccgg aaagagcauc cagccugaaa aucucgaaua ccggauuaug cugucugugc     1080

acggcuccca acacagcggg augaucguua acgauacagg acaugagacc gacgagaaua     1140

gagcuaaagu ggaaauuacg ccgaacucuc cccgcgccga ggccacacuu ggcggcuucg     1200

ggagccuggg acucgauugu gaaccucgga ccggccugga cuuuuccgau cuuuauuacc     1260

ugaccaugaa uaacaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc uggggccgau acaggaacac cccauuggaa uaacaaggaa gcgcuggugg     1380

aguuuaaaga cgcucacgcc aagagacaga ccgucguggu ucucggcucu caggaggggg     1440

ccgugcauac ggcucuggcc ggcgcucuug aagccgagau ggauggagcg aagggccgcc     1500

ugagcuccgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguca     1560

gcuauucucu cuguaccgcc gcuuucacau uuaccaaaau uccggccgaa acacugcaug     1620

gcaccgugac gguugaggug caguacgcug ggaccgacgg accuugcaag guccccgccc     1680

aaauggcggu ggauaugcag acacugaccc cugugggccg ccucaucaca gccaaccccg     1740

uuaucaccga auccacggag aauagcaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acucuuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaggcu uuugaagcca cagugagagg agcuaaacgc auggccgucc     1920

ugggcgacac cgccugggau uucggguccg ugggaggcgc ucucaacucu cuggggaaag     1980

gcauucacca gaucuucgga gcggccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu uauuggaacc cuccugaugu ggcugggccu caauacaaag aacgggagca     2100

ucucccugau gugucuugcu cugggaggcg uucucauuuu ccugagcacg gccgugucug     2160

ccgacgucgg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6753
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 2 opt

<400>  6753
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaucugac gccggggagg cuauuagcuu ccccacaacc cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca auguccuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaacacca      360

caucuaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcagca      420

gacgggccgu uacccuuccc ucccauucua cacgcaagcu gcaaaccaga agccagacau      480

ggcucgaguc ccgggaauac accaagcacc ugauccgcgu ggagaauugg aucuuuagaa      540

acccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggagc ucuacguccc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacagc auucggugca      660

ucggaguuuc uaaucgcgau uuuguggaag gcauguccgg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccguua uggcucagga uaagccuaca guggacauug      780

agcuugugac caccacaguc agcaacaugg ccgaagugag aucuuacugc uaugaggcuu      840

ccaucagcga uauggccucc gacucucggu gucccacgca aggagaagcc uaccuggaca      900

aacagagcga uacccaguau guuugcaaga gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucccugg ugaccugcgc uaaguuugcg ugcagcaaga     1020

aaaugacagg aaagucuauc cagccugaga accucgaaua ccggauuaug cuguccgucc     1080

acggcagcca acacucuggg augaucguua augauaccgg acaugagacg gacgagaaca     1140

gagccaaagu ggaaauuaca ccgaauagcc cccgcgcuga ggccacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gaaccucgga ccggccugga cuuuucugau cuuuauuacc     1260

ugacaaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa gcccuggugg     1380

aguuuaaaga cgcgcacgcu aagagacaga cggucguggu ucucggcagc caggaggggg     1440

ccgugcauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

uguccagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

cuuauucccu cuguacagcg gccuucaccu uuacaaaaau uccggcugaa acccugcaug     1620

gcacggugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacac cugugggccg ccucaucacc gcgaaccccg     1740

uuaucacgga aagcacagag aacucuaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acuccuacau ugucaucggc gugggagaga aaaagauuac ccaccacugg caucggagcg     1860

gcuccacaau cgggaaagcc uuugaagcua ccgugagagg agccaagcgc auggcugucc     1920

ugggcgacac cgccugggau uucgggucug ugggaggcgc ccucaauagc cuggggaaag     1980

gcauucacca gaucuucgga gcugcguuua agucccuguu cggcgggaug ucuugguuua     2040

gccaaauccu uauuggaaca cuccugaugu ggcugggccu caacacgaag aaugggucca     2100

ucagccugau gugucuugcc cugggaggcg uucucauuuu ccugucuacc gcuguguccg     2160

ccgacgucgg gugcagcgug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6754
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 2 opt

<400>  6754
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcaccga cacaagcgug gggaucgucg      120

gacugcuccu gacgaccgcu auggccgcgg aaguuacacg gcgcggcucc gcuuacuaua      180

uguaccuuga uagaucugac gccggggagg ccauuagcuu ccccaccaca cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgcuacc auguccuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaacacaa      360

ccucuacgug ggucguguau ggaaccuguc aucacaagaa aggcgaggcc cggcgcagca      420

gacgggcugu uacacuuccc ucccauucua cccgcaagcu gcaaacaaga agccagaccu      480

ggcucgaguc ccgggaauac acgaagcacc ugauccgcgu ggagaauugg aucuuuagaa      540

acccuggcuu cgcccuggcg gccgcugcca uugcuuggcu ccuggggagc ucuacauccc      600

agaaagucau cuaucuugug augauucugc ucaucgcccc cgcguacagc auucggugca      660

ucggaguuuc uaaucgcgau uuuguggaag gcauguccgg gggaaccugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcccagga uaagccuacc guggacauug      780

agcuugugac cacaacgguc agcaacaugg cugaagugag aucuuacugc uaugaggccu      840

ccaucagcga uauggcuucc gacucucggu gucccaccca aggagaagcc uaccuggaca      900

aacagagcga uacacaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucccugg ucacaugcgc caaguuugcu ugcagcaaga     1020

aaaugaccgg aaagucuauc cagccugaga accucgaaua ccggauuaug cuguccgugc     1080

acggcagcca acacucuggg augaucguua augauacggg acaugagaca gacgagaaca     1140

gagcgaaagu ggaaauuacc ccgaauagcc cccgcgccga ggcuacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gaaccucgga caggccugga cuuuucugau cuuuauuacc     1260

ugaccaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacac aggggccgau acgggaacac cccauuggaa caauaaggaa gcccuggugg     1380

aguuuaaaga cgcucacgcc aagagacaga ccgucguggu ucucggcagc caggaggggg     1440

cggugcauac cgcucuggcc ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

uguccagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

cuuauucccu cuguacagcu gccuucaccu uuacgaaaau uccggcggaa acccugcaug     1620

gcacagugac cguugaggug caguacgccg ggacagacgg accuugcaag guccccgcuc     1680

aaauggccgu ggauaugcag acccugacgc cugugggccg ccucaucaca gcuaaccccg     1740

uuaucaccga agggacagag aacagcaaga ugaugcugga gcuugauccc ccuuucggcg     1800

acucuuacau ugucaucgga gugggcgaga aaaagauuac ccaccacugg caucgguccg     1860

ggagcaccau cggaaaagcc uuugaagcga cagugagagg cgccaagcgc auggcugucc     1920

ugggggacac ggccugggau uucggauccg ugggcggggu ucucaauucu cugggcaaag     1980

gcauucacca gaucuucgga gcugccuuua agagccuguu cgggggaaug uccugguuuu     2040

cucaaauccu uauuggcacc cuccugaugu ggcuggggcu caacacaaag aauggaagca     2100

ucucccugau gugucuugcc cugggcgggg ugcucauuuu ccugagcacc gcugucucug     2160

cggacguggg cugcuccguu gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6755
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 2 opt

<400>  6755
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcggcug aaguuacccg gcgcggcucc gccuacuaua      180

uguaccuuga uagaaaugac gccggggagg cuauuucuuu ccccacaacc cugggaauga      240

acaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca augagcuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaauacca      360

cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu uacccuuccc agccauucca cacgcaagcu gcaaaccaga ucucagacau      480

ggcucgagag ccgggaauac accaagcacc ugauccgcgu ggagaacugg aucuuuagaa      540

auccuggcuu cgcgcuggcc gcugccgcua uugccuggcu ccuggggucc agcacgucuc      600

agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc auucggugca      660

ucggaguuag caaccgcgau uuuguggaag gcaugucugg gggaacaugg gucgacgugg      720

ugcuggagca uggcgggugu gucaccgcua uggcccagga uaagccuaca guugacauug      780

agcuugugac caccacagug uccaauaugg cugaagucag aagcuacugc uaugaggccu      840

cuaucuccga uauggccagc gacucccggu gucccacgca aggagaggcu uaccuggaca      900

aacagucuga uacccaguau gugugcaaga gaacacucgu ugaccgcggc uggggcaacg      960

gguguggacu guucggcaaa gggagccugg ugaccugcgc gaaguuugcc ugcuccaaga     1020

aaaugacagg aaagagcauc cagccugaaa aucucgaaua ccggauuaug cugucugucc     1080

acggcuccca acacagcggg augcuuguga acgauaccgg acaugagacg gacgagaaua     1140

gagcuaaagu ugaaaucaca ccgaacucuc cccgcgccga ggccacccug ggcggcuucg     1200

ggagccucgg acuggauugu gaaccucgga ccggccuuga cuuuuccgau cuguauuacc     1260

ucacaaugaa uaacaagcac uggcuggcuc auaaagagug guuccacgac auuccccugc     1320

cuuggcacgc cggggcggcu accggaacac cccauuggaa uaacaaggaa gcccucgugg     1380

aguuuaaaga ugcccacgcu aagagacaga cggucguggu ccugggcucu caggaggggg     1440

ccgugcauac cgcucuugcc ggcgcgcugg aagccgagau ggacggagcu aagggccgcc     1500

ucagcuccgg gcaccugaaa ugccggcuua agauggauaa acugagacuc aagggaguua     1560

gcuauucucu guguacagcc gcuuucaccu uuacaaaaau cccggccgaa accguggacg     1620

gcacggucac cguggagggg caguacggag gcacagacgg gccuugcaag guucccgcgc     1680

aaauggccgu ggauaugcag acccugacac cugucggccg ccucauuacc gcuaaccccg     1740

ugaucacgga auccacagag aauagcaaga ugaugcugga gcuugauccc ccuuucggag     1800

acucuuacau cgugauuggc guuggggaga aaaagaucac ccaucacugg caccgguccg     1860

gaagcacaau uggcaaagcc uuugaagcua ccgucagagg ggccaagcgc auggccgugc     1920

ugggagacac cgcuugggau uucggcuccg ucgggggcgc gcucaacucu cugggaaaag     1980

gcauccauca gauuaucggg gccgcuuuca agagccuguu uggaggcaug uccugguucu     2040

cucaaauccu uauugggaca cuccugaugu ggcugggacu caauacgaag aacggcagca     2100

ucucccugau gugucuugcc cucgggggcg ugcugauuuu ucugagcacc gccgugucug     2160

gaggcguugg gugcuccgug gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6756
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 2 opt

<400>  6756
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcaccga cacaagcgug gggaucgucg      120

gacugcuccu gacgaccgcu auggccgcgg aaguuacacg gcgcggcucc gcuuacuaua      180

uguaccuuga uagaucugac gccggggagg ccauuagcuu ccccaccaca cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgcuacc auguccuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaacacaa      360

ccucuacgug ggucguguau ggaaccuguc aucacaagaa aggcgaggcc cggcgcagca      420

gacgggcugu uacacuuccc ucccauucua cccgcaagcu gcaaacaaga agccagaccu      480

ggcucgaguc ccgggaauac acgaagcacc ugauccgcgu ggagaauugg aucuuuagaa      540

acccuggcuu cgcccuggcg gccgcugcca uugcuuggcu ccuggggagc ucuacauccc      600

agaaagucau cuaucuugug augauucugc ucaucgcccc cgcguacagc auucggugca      660

ucggaguuuc uaaucgcgau uuuguggaag gcauguccgg gggaaccugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcccagga uaagccuacc guggacauug      780

agcuugugac cacaacgguc agcaacaugg cugaagugag aucuuacugc uaugaggccu      840

ccaucagcga uauggcuucc gacucucggu gucccaccca aggagaagcc uaccuggaca      900

aacagagcga uacacaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucccugg ucacaugcgc caaguuugcu ugcagcaaga     1020

aaaugaccgg aaagucuauc cagccugaga accucgaaua ccggauuaug cuguccgugc     1080

acggcagcca acacucuggg augaucguua augauacggg acaugagaca gacgagaaca     1140

gagcgaaagu ggaaauuacc ccgaauagcc cccgcgccga ggcuacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gaaccucgga caggccugga cuuuucugau cuuuauuacc     1260

ugaccaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggccgau acaggaacgc cccauuggaa caauaaggaa gcucuggugg     1380

aguuuaaaga cgcccacgcg aagagacaga ccgucguggu ucucggcagc caggaggggg     1440

cugugcauac agcccuggcc ggcgcucuug aagccgagau ggauggagcu aagggccgcc     1500

uguccagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

cuuauucccu cuguaccgcc gcguucacau uuagcaaaau uccggccgaa acccugcaug     1620

gcacggugac cgaggaaguu caguacgcug ggacagacgg accuugcaag gugcccgccc     1680

aaauggcugu cgauaugcag acccugacac cugugggccg ccucaucacc gccaaccccg     1740

ugaucacgga gucuacagag aacuccaaga ugaugcugga acuugauccc ccuuucgggg     1800

acagcuacau uguuaucggc gucggagaga aaaagauuac ccaccacugg caucgguccg     1860

gcucuacaau cgggaaagcg uuugaagcca ccgugagagg agcuaagcgc auggccgugc     1920

ugggcgacac cgcuugggau uucgggagcg ucggaggcgc ccucaauucc cuggggaaag     1980

gcauucacca gaucuucgga gccgcuuuua agucucuguu cggcgggaug agcugguuuu     2040

cccaaauccu uauuggaaca cuccugaugu ggcugggccu caacacgaag aaugggagca     2100

ucucucugau gugucuugcg cugggaggcg ugcucauuuu ccuguccacc gccguuagcg     2160

cugacguggg gugcucuguc gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6757
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 2 opt

<400>  6757
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcaccga cacaagcgug gggaucgucg      120

gacugcuccu gacgaccgcu auggccgcgg aaguuacacg gcgcggcucc gcuuacuaua      180

uguaccuuga uagaucugac gccggggagg ccauuagcuu ccccaccaca cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgcuacc auguccuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaacacaa      360

ccucuacgug ggucguguau ggaaccuguc aucacaagaa aggcgaggcc cggcgcagca      420

gacgggcugu uacacuuccc ucccauucua cccgcaagcu gcaaacaaga agccagaccu      480

ggcucgaguc ccgggaauac acgaagcacc ugauccgcgu ggagaauugg aucuuuagaa      540

acccuggcuu cgcccuggcg gccgcugcca uugcuuggcu ccuggggagc ucuacauccc      600

agaaagucau cuaucuugug augauucugc ucaucgcccc cgcguacagc auucggugca      660

ucggaguuuc uaaucgcgau uuuguggaag gcauguccgg gggaaccugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcccagga uaagccuacc guggacauug      780

agcuugugac cacaacgguc agcaacaugg cugaagugag aucuuacugc uaugaggccu      840

ccaucagcga uauggcuucc gacucucggu gucccaccca aggagaagcc uaccuggaca      900

aacagagcga uacacaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucccugg ucacaugcgc caaguuugcu ugcagcaaga     1020

aaaugaccgg aaagucuauc cagccugaga accucgaaua ccggauuaug cuguccgugc     1080

acggcagcca acacucuggg augaucguua augauacggg acaugagaca gacgagaaca     1140

gagcgaaagu ggaaauuacc ccgaauagcc cccgcgccga ggcuacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gaaccucgga caggccugga cuuuucugau cuuuauuacc     1260

ugaccaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggccgau acaggaacgc cccauuggaa caauaaggaa gcucuggugg     1380

aguuuaaaga cgcccacgcg aagagacaga ccgucguggu ucucggcagc caggaggggg     1440

cugugcauac agcccuggcc ggcgcucuug aagccgagau ggauggagcu aagggccgcc     1500

uguccagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

cuuauucccu cuguaccgcc gcguucacau uuaccaaaau uccggccgaa acgcugcaug     1620

gcaccgugac aguugaggug caguacgcug ggaccgacgg accuugcaag guccccgccc     1680

aaauggcugu ggauaugcag acacugaccc cugugggccg ccucaucacg gccaaccccg     1740

uuaucacaga aagcaccgag aacucuaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acuccuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucggagcg     1860

gcuccaccau cgggaaagcg uuugaagcca ccgugagagg agcuaagcgc auggccgucc     1920

ugggcgacac agcuugggau uucgggucug ugggaggcgc ccucaauagc cuggggaaag     1980

gcauucacca gaucuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuua     2040

gccaaauccu uauuggaacg cuccugaugu ggcugggccu caacaccaag aaugggucca     2100

ucagccugau gugucuugcg cugggaggcg uucucauuuu ccugucuaca gccguguccg     2160

cugacgucgg gugcagcgug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6758
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 2 opt

<400>  6758
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcaccga cacaagcgug gggaucgucg      120

gacugcuccu gacgaccgcu auggccguug aagugacacg gcgcggcaau gcguacuaua      180

uguaccuuga uagauccgac gcuggggagg ccauuucuuu ccccaccaca augggaauga      240

acaaaugcua uauccagauu auggaucugg gccacaugug ugacgccacc augagcuacg      300

aaugcccuau gcucgaugag gggguggaac cggacgaugu cgacuguugg ugcaauacaa      360

ccuccacgug ggugguuuau ggaaccuguc aucacaagaa aggcgaggcu cggcgcucua      420

gacgggccgu gacacugccc agccauucca cccgcaaacu ucaaacaaga ucucagaccu      480

ggcuggagag ccgggaauac acgaagcacc ucauccgcgu cgagaacugg aucuuuagaa      540

auccuggcuu cgcucuggcc gcggccgcua uugccuggcu gcucgggucc agcacaucuc      600

agaaagugau cuaucugguu augauucuuc ugaucgcucc cgccuacucc auucggugca      660

ucggagugag caaccgcgau uuugucgaag gcaugucugg gggaaccugg guggacgugg      720

uccucgagca uggcgggugu guuacaguga uggcgcagga uaagccuacc guggacauug      780

agcuggucac cacaacggug uccaauaugg ccgaaguuag aagcuacugc uaugaggcuu      840

cuaucuccga uauggccagc gacucccggu gucccaccca aggagaggcu uaccuugaca      900

aacagucuga uacacaguau gugugcaaga gaacccuggu cgaccgcggc uggggcaacg      960

gguguggacu cuucggcaaa gggagccugg ugacaugcgc caaguuugcc ugcuccaaga     1020

aaaugaccgg aaagagcauc cagccugaaa aucuggaaua ccggauuaug cucucuguuc     1080

acggcuccca acacagcggg augaucguga acgauacggg acaugagaca gacgagaaua     1140

gagcuaaagu cgaaauuacc ccgaacucuc cccgcgcgga ggccacccug ggcggcuucg     1200

ggagccuugg acuggauugu gaaccucgga caggccucga cuuuuccgau cuguauuacc     1260

uuaccaugaa uaacaagcac uggcuggugc auaaagagug guuccacgac aucccccucc     1320

cuuggcacgc uggggccgau acaggaacgc cccauuggaa uaacaaggaa gcccuggucg     1380

aguuuaaaga cgcucacgcc aagagacaga ccgugguugu gcugggcucu caggaggggg     1440

cgguccauac agcucucgcc ggcgcccugg aagcugagau ggauggagcc aagggccgcc     1500

uuagcuccgg gcaccugaaa ugccggcuca agauggacaa acugagacuu aagggaguga     1560

gcuauucucu guguaccgcu gccuucacau uuaccaaaau uccggcggaa acgcuccaug     1620

gcaccguuac aguggagguc caguacgccg ggaccgacgg accuugcaag gugcccgcuc     1680

aaauggccgu ggauaugcag acacugaccc cuguuggccg ccugaucacg gcuaaccccg     1740

ucaucacaga auccaccgag aauagcaaga ugaugcucga gcuggauccc ccuuucgggg     1800

acucuuacau ugugaucggc gugggagaaa agaagauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaagcc uuugaggcga ccgucagagg agccaagcgc auggcugugc     1920

uuggcgacac agccugggau uucggguccg uuggaggcgc ucugaacucu cucgggaaag     1980

gcauucacca gaucuucgga gccgccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu gauuggaacg cuucucgugu ggcugggccu gaauaccaag aacgggagca     2100

ucucccucau gugucuggcu cuuggaggcg uccugauuuu ccucagcaca gcggugucug     2160

ccgacguugg gugcuccgug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6759
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 2 opt

<400>  6759
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcaccga cacaagcgug gggaucgucg      120

gacugcuccu gacgaccgcu auggccgcgg aaguuacacg gcgcggcucc gcuuacuaua      180

uguaccuuga uagaucugac gccggggagg ccauuagcuu ccccaccaca cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgcuacc auguccuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg ugcaacacaa      360

ccucuacgug ggucguguau ggaaccuguc aucacaagaa aggcgaggcc cggcgcagca      420

gacgggcugu uacacuuccc ucccauucua cccgcaagcu gcaaacaaga agccagaccu      480

ggcucgaguc ccgggaauac acgaagcacc ugauccgcgu ggagaauugg aucuuuagaa      540

acccuggcuu cgcccuggcg gccgcugcca uugcuuggcu ccuggggagc ucuacauccc      600

agaaagucau cuaucuugug augauucugc ucaucgcccc cgcguacagc auucggugca      660

ucggaguuuc uaaucgcgau uuuguggaag gcauguccgg gggaaccugg gucgacgugg      720

ugcuggagca uggcgggugu gucacaguua uggcccagga uaagccuacc guggacauug      780

agcuugugac cacaacgguc agcaacaugg cugaagugag aucuuacugc uaugaggccu      840

ccaucagcga uauggcuucc gacucucggu gucccaccca aggagaagcc uaccuggaca      900

aacagagcga uacacaguau guuugcaaga gaacccucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucccugg ucacaugcgc caaguuugcu ugcagcaaga     1020

aaaugaccgg aaagucuauc cagccugaga accucgaaua ccggauuaug cuguccgugc     1080

acggcagcca acacucuggg augaucguua augauacggg acaugagaca gacgagaaca     1140

gagcgaaagu ggaaauuacc ccgaauagcc cccgcgccga ggcuacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gaaccucgga caggccugga cuuuucugau cuuuauuacc     1260

ugaccaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggccgau acaggaacgc cccauuggaa caauaaggaa gcucuggugg     1380

aguuuaaaga cgcccacgcg aagagacaga ccgucguggu ucucggcagc caggaggggg     1440

cugugcauac agcccuggcc ggcgcucuug aagccgagau ggauggagcu aagggccgcc     1500

uguccagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagucu     1560

cuuauucccu cuguaccgcc gcguucacau uuaccaaaau uccggccgaa acgcugcaug     1620

gcaccgugac aguugaggug caguacgcug ggaccgacgg accuugcaag guccccgccc     1680

aaauggcugu ggauaugcag acacugaccc cugugggccg ccucaucacg gccaaccccg     1740

uuaucacaga aagcaccgag aacucuaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acuccuacau ugucaucggc gugggagaga aaaagauuac acaccacugg caucggagcg     1860

gcuccaccau cgggaaagcg uuugaagcca ccgugagagg agcuaagcgc auggccgucc     1920

ugggcgacac agcuugggau uucgggucug ugggaggcgc ccucaauagc cuggggaaag     1980

gcauucacca gaucuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuua     2040

gccaaauccu uauuggaacg cuccugaugu ggcugggccu caacaccaag aaugggucca     2100

ucagccuggu uugucuugcg cugggaggcg ugcucauuuu ccugucuaca gccgucuccg     2160

cugacguggg gugcagcguu gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6760
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 2 opt

<400>  6760
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcaccga cacaagcgug gggaucgucg      120

gacugcuccu gacgaccgcu auggccguug aagugacacg gcgcggcucc gcguacuaua      180

uguaccuuga uagaucugac gcuggggagg ccauuagcuu ccccaccaca cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccacc auguccuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu cgacuguugg ugcaacacaa      360

ccucuacgug ggugguuuau ggaaccuguc aucacaagaa aggcgaggcu cggcgcagca      420

gacgggccgu gacacuuccc ucccauucua cccgcaagcu gcaaacaaga agccagaccu      480

ggcucgaguc ccgggaauac acgaagcacc ugauccgcgu cgagaauugg aucuuuagaa      540

acccuggcuu cgcucuggcc gcggccgcua uugccuggcu ccuggggagc ucuacauccc      600

agaaagugau cuaucuuguu augauucugc ucaucgcucc cgccuacagc auucggugca      660

ucggaguguc uaaucgcgau uuugucgaag gcauguccgg gggaaccugg guggacgugg      720

uccuggagca uggcgggugu guuacaguga uggcgcagga uaagccugcc guggacauug      780

agcuugucac caccacagug agcaacaugg cugaaguuag aucuuacugc uaugaggccu      840

ccaucagcga uauggcuucc gacucucggu gucccacgca aggagaagcc uaccuggaca      900

aacagagcga uacccaguau gugugcaaga gaacacucgu cgaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucccugg ugaccugcgc caaguuugcu ugcagcaaga     1020

aaaugacagg aaagucuauc cagccugaga accucgaaua ccggauuaug cuguccguuc     1080

acggcagcca acacucuggg augaucguga augauaccgg acaugagacg gacgagaaca     1140

gagcgaaagu cgaaauuaca ccgaauagcc cccgcgccga ggcuacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gaaccucgga ccggccugga cuuuucugau cuuuauuacc     1260

ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggccgau accggaacac cccauuggaa caauaaggaa gcucuggucg     1380

aguuuaaaga cgcccacgcg aagagacaga cggugguugu gcucggcagc caggaggggg     1440

cuguccauac cgcccuggcc ggcgcucuug aagccgagau ggauggagcu aagggccgcc     1500

uguccagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggagugu     1560

cuuauucccu cuguacagcc gcguucaccu uuacaaaaau uccggccgaa acccugcaug     1620

gcacgguuac cguggagguc caguacgcug ggacagacgg accuugcaag gugcccgccc     1680

aaauggcugu ggauaugcag acccugacac cuguuggccg ccucaucacc gccaaccccg     1740

ucaucacgga aagcacagag aacucuaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acuccuacau ugugaucggc gugggagaga aaaagauuac ccaccacugg caucggagcg     1860

gcuccacaau cgggaaagcg uuugaagcca ccgucagagg agcuaagcgc auggccgugc     1920

ugggcgacac cgcuugggau uucgggucug uuggaggcgc ccucaauagc cuggggaaag     1980

gcauucacca gaucuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuua     2040

gccaaauccu uauuggaaca cuccuggugu ggcugggccu caacacgaag aaugggucca     2100

ucagccugac cugucuugcg cugggaggcg uccucauuuu ccugucuaca gccguguccg     2160

cugacguugg gugcagcgug gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6761
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 2 opt

<400>  6761
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcgguug aagugacccg gcgcggcucc gcuuacuaua      180

uguaccuuga uagaucugac gccggggagg ccauuagcuu ccccacaacc cugggaauga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgcuaca auguccuacg      300

aaugcccuau gcuggaugag gggguggaac cggacgaugu cgacuguugg ugcaacacca      360

caucuaccug ggugguuuau ggaacguguc aucacaagaa aggcgaggcc cggcgcagca      420

gacgggcugu gacccuuccc ucccauucua cacgcaagcu gcaaaccaga agccagacau      480

ggcucgaguc ccgggaauac accaagcacc ugauccgcgu cgagaauugg aucuuuagaa      540

acccuggcuu cgcccuggcg gccgcuguga uugccuggcu ccuggggagc ucuacguccc      600

agaaaguuau cuaucuugug augauucugc ucaucgcucc cgccuacagc auucggugca      660

ucggagucuc uaaucgcgau uuuguggaag gcauguccgg gggaacaugg guggacgucg      720

uucuggagca uggcgggugu gugaccguga uggcgcagga uaagccuaca gucgacauug      780

agcuugugac caccacaguu agcaacaugg ccgaagugag aucuuacugc uaugaggcuu      840

ccaucagcga uauggccucc gacucucggu gucccacgca aggagaggcu uaccuggaca      900

aacagagcga uacccaguau gucugcaaga gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucccugg uuaccugcgc caaguuugcc ugcagcaaga     1020

aaaugacagg aaagucuauc cagccugaaa accucgaaua ccggauuaug cuguccgugc     1080

acggcagcca acacucuggg augaucguca augauaccgg acaugagacg gacgagaaca     1140

gagcuaaagu ggaaauuaca ccgaauagcc cccgcgcgga ggccacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gaaccucgga ccggccugga cuuuucugau cuuuauuacc     1260

ugacaaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc uggggccgau accggaacac cccauuggaa caauaaggaa gcccuggugg     1380

aguuuaaaga cgcucacgcc aagagacaga cgguuguggu ccucggcagc caggaggggg     1440

cggugcauac cgcucuggcc ggcgcccuug aagcugagau ggauggagcc aagggccgcc     1500

uguccagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguuu     1560

cuuauucccu cuguacagcu gccuucaccu uuacaaaaau uccggcggaa acccugcaug     1620

gcacggugac cgucgaggug caguacgccg ggacagacgg accuugcaag gugcccgcuc     1680

aaauggccgu ugauaugcag acccugacac cugucggccg ccucaucacc gcuaaccccg     1740

ugaucacgga aagcacagag aacucuaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acuccuacau ugugaucggc gucggagaga aaaagauuac ccaccacugg caucggagcg     1860

gcuccacaau cgggaaagcc uuugaagcga ccgugagagg agccaagcgc auggcuguuc     1920

ugggcgacac cgccugggau uucgggucug ugggaggcgc ucucaauagc cuggggaaag     1980

gcauucacca gaucuucgga gccgccuuua agucccuguu cggcgggaug ucuugguuua     2040

gccaaauccu uauuggaaca cuccuggucu ggcugggccu caacacgaag aaugggucca     2100

ucagccugac cugucuugcu cugggaggcg ugcucauuuu ccugucuaca gcgguuuccg     2160

ccgacguggg gugcagcguc gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6762
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 2 opt

<400>  6762
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcgguug aagugacccg gcgcggcucc gcuuacuaua      180

uguaccuuga uagaucugac gccggggagg ccauuagcuu ccccacaacc cugggaguga      240

auaaaugcua uauccagauu auggaucucg gccacaugug ugacgcuaca auguccuacg      300

aaugcccuau gcuggaugag ggggucgaac cggacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguuguguau ggaacguguc aucacaagaa aggcgaggcc cggcgcagca      420

gacgggcugu cacccuuccc ucccauucua cacgcaagcu gcaaaccaga agccagacau      480

ggcucgaguc ccgggaauac accaagcacc ugauccgcgu ggagaauugg aucuuuagaa      540

acccuggcuu cgcccuggcg gccgcugcca uugcuuggcu ccuggggagc ucuacguccc      600

agaaaguuau cuaucuugug augauucugc ucaucgcccc cgcguacagc auucggugca      660

ucggagucuc uaaucgcgau uuuguggaag gcauguccgg gggaacaugg guggacgucg      720

uucuggagca uggcgggugu gugaccguga uggcccagga uaagccuaca gucgacauug      780

agcuugugac caccacaguu agcaacaugg cugaagugag aucuuacugc uaugaggccu      840

ccaucagcga uauggcuucc gacucucggu gucccacgca aggagaagcc uaccuggaca      900

aacagagcga uacccaguau gucugcaaga gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaaa gggucccugg uuaccugcgc caaguuugcu ugcagcaaga     1020

aaaugacagg aaagucuauc cagccugaga accucgaaua ccggauuaug cuguccgugc     1080

acggcagcca acacucuggg augaucguca augauaccgg acaugagacg gacgagaaca     1140

gagcgaaagu ggaaauuaca ccgaauagcc cccgcgccga ggcuacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gaaccucgga ccggccugga cuuuucugau cuuuauuacc     1260

ugacaaugaa caauaagcac uggcucgucc auaaagagug guuccacgac aucccccugc     1320

cuuggcacgc cggggccgau accggaacac cccauuggaa caauaaggaa gcucuggugg     1380

aguuuaaaga cgcccacgcg aagagacaga cgguuguggu ccucggcagc caggaggggg     1440

cugugcauac cgcccuggcc ggcgcucuug aagccgagau ggauggagcu aagggccgcc     1500

uguccagcgg gcaccucaaa ugccggcuga agauggacaa acuuagacug aagggaguuu     1560

cuuauucccu cuguacagcc gcguucaccu uuacaaaaau uccggccgaa acccugcaug     1620

gcacggugac cgucgaggug caguacgcug ggacagacgg accuugcaag gugcccgccc     1680

aaauggcugu ugauaugcag acccugacac cugucggccg ccucaucacc gccaaccccg     1740

ugaucacgga aagcacagag aacucuaaga ugaugcugga gcuugauccc ccuuucgggg     1800

acuccuacau ugugaucggc gucggagaca aaaagauuac ccaccacugg uaucggagcg     1860

gcuccacaau cgggaaagcg uuugaagcca ccgugagagg agcuaagcgc auggccguuc     1920

ugggcgauac cgcuugggac uucgggucug ugggaggcgc ccucaauagc cuggggaaag     1980

gcauucauca gaucuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuua     2040

gccaaauccu uauuggaaca cuccuggucu ggcugggccu caacacgaag aaugggucca     2100

ucagccugac cugucuugcg cugggaggcg ugcucauuuu ccugucuaca gccguuuccg     2160

cugauguggg gugcagcguc gacuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6763
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 2 opt

<400>  6763
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag aaaaagcgca gaggcgcuga caccagcgug gggaucgucg      120

gacugcuccu gacaacggcc auggcgguug aagugacccg gcgcggcucc gcuuacuaua      180

uguaccuuga uagaucugac gccgggaaag ccauuagcuu ccccacaacc cugggaguga      240

auaagugcua uauccagauu auggaucucg gccacaugug ugacgcuaca auguccuacg      300

agugcccuau gcuggaugaa ggggucgagc cggacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguuguguau ggaacguguc aucacaaaaa gggcgaagcc cggcgcagca      420

gacgggcugu cacccuuccc ucccauucua cacgcaagcu gcaaaccaga agccagacau      480

ggcucgaguc ccgggaguac accaaacacc ugauccgcgu ggaaaauugg aucuuuagaa      540

acccuggcuu cgcccuggcg gccgcugcca uugcuuggcu ccuggggagc ucuacguccc      600

agaagguuau cuaucuugug augauucugc ucaucgcccc cgcguacagc auucggugca      660

ucggagucuc uaaucgcgau uuuguggagg gcauguccgg gggaacaugg guggacgucg      720

uucuggaaca uggcgggugu gugaccguga uggcccagga uaaaccuaca gucgacauug      780

agcuugugac caccacaguu agcaacaugg cugaggugag aucuuacugc uaugaagccu      840

ccaucagcga uauggcuucc gacucucggu gucccacgca aggagaggcc uaccuggaca      900

agcagagcga uacccaguau gucugcaaaa gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg uuaccugcgc caaguuugcu ugcagcaaaa     1020

agaugacagg aaaaucuauc cagccugaaa accucgagua ccggauuaug cuguccgugc     1080

acggcagcca acacucuggg augaucguca augauauugg acaugaaacc gacgagaaca     1140

gagcgaaggu ggagaucacg ccgaauagcc cccgcgccga agcuacacuu ggcggcuucg     1200

ggucccuggg acucgauugu gagccucgga ccggccugga cuuuucugau cuuuauuacc     1260

ugaccaugaa caauaaacac uggcucgucc auaaggaaug guuccacgac auuccccugc     1320

cuuggcacgc cggggccgau acaggaacac cccauuggaa caauaaagag gcucuggugg     1380

aauuuaagga cgcccacgcg aagagacaga caguuguggu ccucggcagc caggaggggg     1440

cugugcauac ggcccuggcc ggcgcucuug aggccgaaau ggauggagcu aaaggccgcc     1500

uguccagcgg gcaccucaag ugccggcuga aaauggacaa acuuagacug aagggaguuu     1560

cuuauucccu cuguaccgcc gcguucacau uuaccaagau cccggccgag acacugcaug     1620

gcaccgugac ggucgaagug caguacgcug ggaccgacgg accuugcaag gugcccgccc     1680

aaauggcugu ugauaugcag acacugaccc cugucggccg ccucaucaca gccaaccccg     1740

ugauuaccga gagcacggaa aacucuaaaa ugaugcugga gcuugauccc ccuuucgggg     1800

acuccuacau cgugauuggc gucggagaca agaaaaucac acaccacugg caucggagcg     1860

gcuccaccau ugggaaggcg uuugaggcca cagugagagg agcuaaacgc auggccguuc     1920

ugggcgauac cgcuugggac uucgggucug ugggaggcgc ccucaauagc cuggggaagg     1980

gcauccacca gaucuucgga gccgcuuuua agucccuguu cggcgggaug ucuugguuua     2040

gccaaauucu uaucggaacc cuccuggucu ggcugggccu caacaccaaa aaugggucca     2100

uuagccugac gugucuugcg cugggaggcg ugcucaucuu ccugucuacc gccguuuccg     2160

cugauguggg gugcagcguc gacuuuucua agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6764
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 2 opt

<400>  6764
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauugugg      120

gacugcuccu gacaacggcc auggcggcug aaaucacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuauuagcuu cgccacaacc cugggaguca      240

auaaaugcca cguucagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguggagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug ggucguguac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu uacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugaucaaagu ggaaaauugg auuuuuagaa      540

accccggcuu cacgcugguc gccguggcua ucgccuggcu ccuggggagc ucuacauccc      600

agaagguuau uuaccuugug augauccugc ucauugcucc ugccuauagc auccggugca      660

uuggagucuc uaaucgcgau uuuguggagg gcauguccgg gggaaccugg guggacgucg      720

uucuggaaca cggcgggugu gugacaguga uggcgcagga uaaacccacc gucgacaucg      780

agcuugugac cacaacgguu agcaacaugg ccgaggugag aucuuacugc uacgaagccu      840

ccaucagcga uauggcuucc gacucucggu guccuaccca aggagaggcu uaucuggaca      900

agcagagcga uacacaguac gucugcaaaa gaacccucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg uuacaugcgc caaguuuacc ugcagcaaaa     1020

agaugacggg aaaaucuauu cagcccgaaa accucgagua ucggaucaug cuguccgugc     1080

acggcagcca acauucuggg augauuguca augauacagg acacgaaacc gacgagaaca     1140

gagccaaggu ggaggucacc ccuaauagcc cgcgcgcuga agcgacacuu ggcggcuucg     1200

ggucccuggg acucgauugu gagccccgga ccggccugga cuuuucugau cuuuacuauc     1260

ugacaaugaa caauaaacau uggcucgugc acaaggaaug guuccacgac aucccucugc     1320

ccuggcaugc cggggcugau acgggaaccc cucacuggaa caauaaagag gcccugguug     1380

aauuuaagga cgcccaugcu aagagacaga caguggucgu gcucggcagc caggaggggg     1440

ccguucacac cgcgcuggcu ggcgcccuug aggccgaaau ggauggagcu aaaggccgcc     1500

uguucuccgg gcaucucaag ugccggcuga aaauggacaa acuuagacug aaaggaguga     1560

gcuacucucu cuguacagcc gcuuuuaccu ucacgaaggu ccccgccgag acccugcacg     1620

gcacagugac cguggaaguu caguaugcgg ggacagacgg accgugcaag gucccugccc     1680

aaauggcugu ggauaugcag acccugacgc ccgugggccg ccucauuaca gccaacccug     1740

ucaucaccga guccacagaa aacagcaaaa ugaugcugga gcuugacccu cccuuugggg     1800

auucuuacau cgugauuggc guuggagaca agaaaaucac ccaccauugg caccgguccg     1860

gcagcaccau ugggaaggcu uucgaggcca cagugagagg agcgaaacgc auggccgucc     1920

ugggcgauac ggcuugggac uucggguccg ugggaggcgu uuuuaauucu cucgggaagg     1980

gcauccauca gauuuucgga gccgcuuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu gaucggaacc cuucucgugu ggcugggccu gaacacaaag aaugggagca     2100

uuucccucac cugucuggcc cuuggaggcg ucaugaucuu ccugagcaca gccgugucug     2160

cugauguugg gugcuccgug gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6765
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 2 opt

<400>  6765
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaaau cugggaguga      240

acaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuacc auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaauacaa      360

ccucuacaug gguggucuac ggaaccuguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu gacgcuuccg ucccacucua ccagaaagcu gcaaacacgg agccagaccu      480

ggcucgaguc ccgcgaguau acaaagcauc ugauuaaagu ugaaaacugg aucuuuagaa      540

aucccggcuu cgcccuggcu gccguggcua uugccuggcu ccuggggagc ucuaccuccc      600

agaaggucau cuaccuugug augauucugc ucaucgcgcc ugccuauagc auucggugca      660

ucggaguuuc uaaccgcgau uuuguggagg gcauguccgg gggaacgugg gucgacgugg      720

ugcuggaaca cggcgggugu gucacaguua uggcucagga uaaacccacc guggacaucg      780

agcuugugac aaccaccguc agcaauaugg ccgaggugag aucuuacugc uacgaggcuu      840

ccauuagcga uauggccucc gacucucggu guccuacaca aggagaagcc uaucuggaca      900

agcagagcga uacgcaguac guuugcaaaa gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaag gggucccugg ucacaugcgc uaaguuuacc ugcagcaaaa     1020

agaugacagg aaaaucuauc cagcccgaaa aucucgagua ucggauuaug cuguccgugc     1080

acggcagcca acauucuggg augaucguua acgauauugg acacgaaacc gacgagaaua     1140

gagcgaaggu ggaggucacg ccuaacagcc cgcgcgccga agcuacacuu ggcggcuucg     1200

ggucccuggg acucgauugu gagccccgga ccggccugga cuuuucugau cuuuacuauc     1260

ugaccaugaa uaacaaacau uggcucgugc acaaggaaug guuccacgac aucccucugc     1320

ccuggcaugc cggggccgau acaggaaccc cucacuggaa uaacaaagag gcucuggucg     1380

aauuuaagga cgcccaugcg aagagacaga cagugguugu gcucggcagc caggaggggg     1440

cuguccacac ggcccuggcc ggcgcucuug aggccgaaau ggauggagcu aaaggccgcc     1500

uguucuccgg gcaucucaag ugccggcuga aaauggacaa acuuagacug aaaggaguga     1560

gcuacucucu cuguaccgcc gcguuuacau ucaccaaggu ucccgccgag acacugcacg     1620

gcaccgugac ggucgaagug caguaugcug ggaccgacgg accgugcaag gugccugccc     1680

aaauggcugu ugauaugcag acacugaccc ccgucggccg ccucaucaca gccaacccug     1740

ugauuaccga guccacggaa aauagcaaaa ugaugcugga gcuugacccu cccuuugggg     1800

auucuuacau cgugauuggc gucggagaca agaaaaucac acaccauugg caccgguccg     1860

gcagcaccau ugggaaggcg uucgaggcca cagugagagg agcuaaacgc auggccguuc     1920

ugggcgauac cgcuugggac uucggguccg ugggaggcgu cuuuaacucu cucgggaagg     1980

gcgugcauca gaucuucgga gccgccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu gauuggaacc cuucucguuu ggcugggccu gaauacaaag aacgggagca     2100

ucucccucac gugucuggcu cuuggaggcg ugaugauuuu ccugagcacc gcggucucug     2160

ccgauguggg gugcuccguu gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6766
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 2 opt

<400>  6766
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc cugggaguga      240

auaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu gacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugauuaaagu ugaaaauugg aucuuuagaa      540

accccggcuu cgcccuggug gcugucgcca uugcuuggcu ccuggggagc ucuacguccc      600

agaaggugau cuaccuuguu augauucugc ucaucgcccc ugcguauagc auucggugca      660

ucggaguguc uaaucgcgau uuugucgagg gcauguccgg gggaacaugg guggacgugg      720

uccuggaaca cggcgggugu guuaccguga uggcccagga uaaacccaca guggacaucg      780

agcuugucac caccacagug agcaacaugg cugagguuag aucuuacugc uacgaagccu      840

ccauuagcga uauggcuucc gacucucggu guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gugugcaaaa gaacacucgu cgaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg ugaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauc cagcccgaaa accucgagua ucggauuaug cuguccguuc     1080

acggcagcca acauucuggg augaucguga augauauugg acacgaaacg gacgagaaca     1140

gagcuaaggu cgaggugaca ccuaauagcc cgcgcgcgga agccacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gagccccgga ccggccugga cuuuucugau cuuuacuauc     1260

ugacaaugaa caauaaacau uggcucgucc acaaggaaug guuccacgac aucccucugc     1320

ccuggcaugc uggggccgau accggaacac cucacuggaa caauaaagag gcccuggugg     1380

aauuuaagga cgcucaugcc aagagacaga cgguuguggu ccucggcagc caggaggggg     1440

cggugcacac cgcucuggcc ggcgcccuug aggcugaaau ggauggagcc aaaggccgcc     1500

uguucuccgg gcaucucaag ugccggcuga aaauggacaa acuuagacug aagggaguua     1560

gcuacucucu cuguacagcu gccuuuaccu ucacaaaggu gcccgcggag acccugcacg     1620

gcacggucac cguggaagug caguaugccg ggacagacgg accgugcaag guuccugcuc     1680

aaauggccgu cgauaugcag acccugacac ccgugggccg ccucaucacc gcuaacccug     1740

ugauuacgga guccacagaa aacagcaaaa ugaugcugga gcuugacccu cccuuugggg     1800

auucuuacau cgucauuggc gugggagaca agaaaaucac ccaccauugg caccgguccg     1860

gcagcacaau ugggaaggcc uucgaggcga ccguuagagg agccaaacgc auggcugugc     1920

ugggcgauac cgccugggac uucggguccg ucggaggcgu guuuaauucu cucgggaagg     1980

gcauccauca gaucuucgga gcugccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauucu gaucggaaca cuucucguuu ggcugggccu gaacacgaaa aaugggagca     2100

uuucccucac cugucuggcc cuuggaggcg ugaugaucuu ccugagcaca gcugucucug     2160

cggauguggg gugcuccguu gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6767
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 2 opt

<400>  6767
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaaau cugggaguga      240

acaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuacc auguccuaug      300

agugccccau gcuggaugaa gggguugagc ccgacgaugu ggacuguugg ugcaauacaa      360

ccucuacaug gguggucuac ggaaccuguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu gacgcuuccg ucccacucua ccagaaagcu gcaaacacgg agccagaccu      480

ggcucgaguc ccgcgaguau acaaagcauc ugauuaaagu ugaaaacugg aucuuuagaa      540

aucccggcuu cgcccuggcu gccguggcua uugccuggcu ccuggggagc ucuaccuccc      600

agaaggucau cuaccuugug augauucugc ucaucgcgcc ugccuauagc auucggugca      660

ucggaguuuc uaaccgcgau uuuguggagg gcauguccgg gggaacgugg gucgacgugg      720

ugcuggaaca cggcgggugu gucacaguua uggcucagga uaaacccacc guggacaucg      780

agcuugugac aaccaccguc agcaauaugg ccgaggugag aucuuacugc uacgaggcuu      840

ccauuagcga uauggccucc gacucucggu guccuacaca aggagaagcc uaucuggaca      900

agcagagcga uacgcaguac guuugcaaaa gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guucggcaag gggucccugg ucacaugcgc uaaguuuacc ugcagcaaaa     1020

agaugacagg aaaaucuauc cagcccgaaa aucucgagua ucggauuaug cuguccgugc     1080

acggcagcca acauucuggg augaucguua acgaugaaaa uagagcgaag guggagguca     1140

ccccuaacag cccgcgcgcc gaggcuacgc uuggaggcuu cggcucccug gggcucgacu     1200

gugaaccccg gacaggacug gauuuuucug accuuuacua ucugaccaug aauaacaaac     1260

acuggcucgu gcauaaggag ugguuccacg auauuccucu gcccuggcac gccggcgccg     1320

acaccgggac accucauugg aauaacaaag aagcucuggu cgaguuuaag gaugcccacg     1380

cgaagagaca gaccgugguu gugcucggaa gccaggaagg cgcuguccau acagcccugg     1440

ccggggcucu ugaggccgag auggacggcg cuaaaggacg ccuguucucc ggccaccuca     1500

agugccggcu gaaaauggau aaacuuagac ugaagggggu gagcuacucu cucuguacgg     1560

ccgcguuuac cuucacaaag guucccgccg aaacccugca uggaacagug accgucgagg     1620

ugcaguaugc uggcacggac gggccgugca aggugccugc ccaaauggcu guugacaugc     1680

agacccugac acccgucgga cgccucauca ccgccaaccc ugugaucaca gaauccaccg     1740

agaauagcaa aaugaugcug gaacuugauc cucccuuugg cgacucuuac auugugaucg     1800

gggucggcga uaagaaaauu acgcaccacu ggcaucgguc cggaagcaca aucggcaagg     1860

cguucgaggc caccgugaga ggggcuaaac gcauggccgu ucugggagac acagcuuggg     1920

auuucggcuc cgucggggga guguuuaacu cucucggcaa ggggauucac cagaucuucg     1980

gcgccgccuu uaagagccug uucggaggca uguccugguu uucucaaauc cugauuggga     2040

cccuucucgu guggcuggga cugaauacca aaaacggcag caucucccuc acaugucugg     2100

cucuuggggg aguuaugauu uuccugagca cggcgguguc ugccgacguc ggcugcuccg     2160

uggauuuuag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6768
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 2 opt

<400>  6768
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauugugg      120

gacugcuccu gacaacggcc auggcggcug aaaucacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuauuagcuu cgccacaacc cugggaguca      240

auaaaugcca cguucagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguggagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug ggucguguac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu uacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugaucaaagu ggaaaauugg auuuuuagaa      540

accccggcuu cgcccugguc gcuguggcca ucgcuuggcu ccuggggagc ucuacguccc      600

agaagguuau uuaccuugug augauccugc ucauugcccc ugcguauagc auccggugca      660

uuggagucuc uaaucgcgau uuuguggagg gcauguccgg gggaacaugg guggacgucg      720

uucuggaaca cggcgggugu gugaccguga uggcccagga uaaacccaca gucgacaucg      780

agcuugugac caccacaguu agcaacaugg cugaggugag aucuuacugc uacgaagccu      840

ccaucagcga uauggcuucc gacucucggu guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gucugcaaaa gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg uuaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauu cagcccgaaa accucgagua ucggaucaug cuguccgugc     1080

acggcagcca acauucuggg augauuguca augauacggg acacgaaaca gacgagaaca     1140

gagcuaaggu ggaggucacc ccuaauagcc cgcgcgcgga agccacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gagccccgga caggccugga cuuuucugau cuuuacuauc     1260

ugaccaugaa caauaaacau uggcucgugc acaaggaaug guuccacgac aucccucugc     1320

ccuggcaugc uggggccgau acaggaacgc cucacuggaa caauaaagag gcccugguug     1380

aauuuaagga cgcucaugcc aagagacaga ccguggucgu gcucggcagc caggaggggg     1440

cgguucacac agcucuggcc ggcgcccuug aggcugaaau ggauggagcc aaaggccgcc     1500

uguucuccgg gcaucucaag ugccggcuga aaauggacaa acuuagacug aagggaguga     1560

gcuacucucu cuguaccgcu gccuuuacau ucaccaaggu ccccgcggag acgcugcacg     1620

gcaccgugac aguggaaguu caguaugccg ggaccgacgg accgugcaag gucccugcuc     1680

aaauggccgu ggauaugcag acacugaccc ccgugggccg ccucauuacg gcuaacccug     1740

ucaucacaga guccaccgaa aacagcaaaa ugaugcugga gcuugacccu cccuuugggg     1800

auucuuacau cgugauuggc guuggagaca agaaaaucac acaccauugg caccgguccg     1860

gcagcaccau ugggaaggcc uucgaggcga ccgugagagg agccaaacgc auggcugucc     1920

ugggcgauac agccugggac uucggguccg ugggaggcgu uuuuaauucu cucgggaagg     1980

gcauccauca gauuuucgga gcugccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucaaauccu gaucggaacg cuucucgugu ggcugggccu gaacaccaaa aaugggagca     2100

uuucccucac augucuggcc cuuggaggcg ucaugaucuu ccugagcacc gcugugucug     2160

cggauguugg gugcuccgug gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6769
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 2 opt

<400>  6769
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauugugg      120

gacugcuccu gacaacggcc auggcggcug aaaucacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuauuagcuu cgccacaacc cugggaguca      240

auaaaugcca cguucagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguggagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug ggucguguac ggaacguguc aacacaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu uacccuuccg ucccauucua caagaaagcu gcagacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcacc ugaucaaagu ggaaaauugg auuuuuagaa      540

accccggcuu cgcccugguc gcuguggcca ucgcuuggcu ccuggggagc ucuacguccc      600

agaagguuau uuaccuugug augauccugc ucauugcccc ugcguauagc auccggugca      660

uuggagucuc uaaucgcgau uuuguggagg gcauguccgg gggaacaugg guggacgucg      720

uucuggaaca uggcgggugu gugaccguga uggcccaaga uaaacccaca gucgacaucg      780

agcuugugac caccacaguu agcaacaugg cugaggugag aucuuacugc uacgaagccu      840

ccaucagcga uauggcuucc gacucucggu guccuacgca gggagaggcc uaucuggaca      900

agcagagcga uacccaguac gucugcaaaa gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg uuaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauu caacccgaaa accucgagua ucggaucaug cuguccgugc     1080

acggcagcca gcacucuggg augauuguca augauaucgg acaugaaacg gacgagaaca     1140

gagcuaaggu ggaggucaca ccuaauagcc cgcgcgcgga agccacccuu ggcggcuucg     1200

ggucccuggg acucgauugu gagccccgga ccggccugga cuuuucugau cuuuacuauc     1260

ugacaaugaa caauaaacac uggcucgugc auaaggaaug guuccacgac auuccucugc     1320

ccuggcacgc uggggccgau accggaacac cucauuggaa caauaaagag gcccugguug     1380

aauuuaagga cgcucacgcc aagagacaga cgguggucgu gcucggcagc caggaggggg     1440

cgguucauac cgcucuggcc ggcgcccuug aggcugaaau ggauggagcc aaaggccgcc     1500

uguucuccgg gcaccucaag ugccggcuga aaauggacaa acuuagacug aaaggaguga     1560

gcuacucucu cuguacagcu gucugcaccg ccgcgaaggu gcccgccgag acacugcaug     1620

gcaccgugac gguugaaguc caauaugcug ggaccgacgg accguguaag gugccugccc     1680

agauggcugu ggauaugcag acacugaccc ccgucggccg ccucaucaca gccaacccug     1740

ugaucaccga guccacggaa aacagcaaaa ugaugcugga gcuugacccu cccuuugggg     1800

auucuuacau uguuaucggc gugggagaca agaaaauuac acaccacugg caucgguccg     1860

gcagcaccau cgggaaggcg uucgaggcca cagucagagg agcuaaacgc auggccgugc     1920

ugggcgauac cgcuugggac uuuggguccg uuggaggcgu guucaauucu cucgggaagg     1980

gcauucacca aaucuucgga gccgccuuua agagccuguu cggcgggaug uccugguuuu     2040

cucagauccu gauuggaacc cuucucgucu ggcugggccu gaacacaaag aaugggagca     2100

ucucccucac gugccuggcu cuuggaggcg ugaugauuuu ccugagcacc gcgguuucug     2160

ccgauguggg guguuccguc gacuuuagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6770
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 2 opt

<400>  6770
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga cgccagcauc gggauugugu      120

cccugcuccu gaccacaguc auggcggcug aaaucacgcg cagaggaucu gccuacuaua      180

uguaccuuga ucggagcgac gccggcaagg cuauuuccuu cguuaccaca cuggggguga      240

auaaaugcca cgugcagauc auggaucucg gacauaugug ugacgccacc augucuuaug      300

agugccccau gcuggaugaa ggcgucgagc ccgacgaugu ggacuguugg ugcaacacaa      360

ccagcacaug gguuguguac gggaccuguc accauaagaa aggagaagcu cgcagauccc      420

ggcgcgccgu cacgcuuccg ucucacagca ccagaaagcu gcaaacacgg ucccagaccu      480

ggcucgagag ccgcgaguau acaaagcauc ugaucaaagu ggaaaauugg auuuuuagaa      540

accccggcuu cgcgcugguu gccguggcua ucgccuggcu ccugggaucu uccaccagcc      600

agaaggucau uuaccuugug augauccugc ucauugcucc ugccuauucu auccggugca      660

uugggguguc caaucgcgau uuugucgagg gaaugagcgg cgggacgugg guugacgugg      720

ugcuggaaca cggaggcugu gucacaguga uggcgcagga uaaacccacc guugacaucg      780

agcuugugac aaccaccguc ucuaacaugg ccgaggugag auccuacugc uacgaagcua      840

gcaucuccga uauggccucu gacagccggu guccuacaca aggggaggcu uaucuggaca      900

agcaguccga uacgcaguac guuugcaaaa gaacccucgu ggaccgcgga uggggcaaug      960

gcugugggcu guucggaaag ggcagccugg ucacaugcgc caaguuuacc ugcucuaaaa     1020

agaugacagg gaaauccauu cagcccgaaa accucgagua ucggaucaug cugagcgugc     1080

acggaucuca acauagcggc augauuguca augaugaaaa cagagccaag guggagguua     1140

ccccuaauuc cccgcgcgcu gaggcgacgc uugggggauu cggcucucug ggccucgacu     1200

gugaaccccg gacagggcug gauuuuagcg accuuuacua ucugaccaug aacaauaaac     1260

acuggcucgu gcauaaggag ugguuccacg auaucccucu gcccuggcac uccggagccg     1320

acaccgaaac accucauugg aacaauaaag aggcucuggu cgaauuuaag gaugcccacg     1380

ccaagagaca gaccgugguu gugcucggca gccaggaggg ggcuguccau acagcccugg     1440

cgggagcucu ugaggccgaa auggacggcg ccaaagggcg ccugucuucc ggccaccuca     1500

agugccggcu gaaaauggau aaacuuagac ugaaaggagu gagcuacucu cucuguacgg     1560

cugccuucac cuuuacaaag gugcccgcug agacccugca uggcacaguu accgucgaag     1620

ugcaguaugc cgggcgcgac ggaccgugca aggugccugc gcaaauggcc gucgacaugc     1680

agacgcugac ccccgugggc cggcucauua cagcuaaccc uguuaucacc gaguccacag     1740

aaaacagcaa aaugaugcug gagcuugauc cucccuucgg ggacuccuac aucgugauug     1800

gagucggcga uaagaaaauc acccaccacu ggcauagauc ugggagcauu aucggcaagg     1860

ccuuugaggc uacggugcgc ggagccaaac ggauggcggu ucugggcgac acagccuggg     1920

auuucggguc cgugggaggc gucuucaauu cucucgggaa gggcauucac cagaucuuug     1980

gcgcugccuu caagagccug uuugggggca uguccugguu cagccaaauc cugauuggaa     2040

cccuucucgu guggcugggc cugaacacaa agaauggguc uaucucccuc accugucugg     2100

cucuuggagg cguuaugauu uuucugagca ccgccguguc ugccgacguc gggugcuccg     2160

uggauuucag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6771
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 2 opt

<400>  6771
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauugugg      120

gacugcuccu gacaacggcc auggcggcug aaaucacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuauuagcuu cgccacaacc cugggaguca      240

auaaaugcca cguucagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguggagc ccgacgaugu ggacuguugg ugcaacacca      360

caucuaccug ggucguguac ggaacguguc accauaagaa aggcgaagcc cgcagaagcc      420

ggcgcgcggu uacccuuccg ucccacucua caagaaagcu gcaaacccgg agccagacau      480

ggcucgaguc ccgcgaguau accaagcauc ugaucaaagu ggaaaauugg auuuuuagaa      540

accccggcuu cgcccugguc gcuguggcca ucgcuuggcu ccuggggagc ucuacguccc      600

agaagguuau uuaccuugug augauccugc ucauugcccc ugcguauagc auccggugca      660

uuggagucuc uaaucgcgau uuuguggagg gcauguccgg gggaacaugg guggacgucg      720

uucuggaaca cggcgggugu gugaccguga uggcccagga uaaacccaca gucgacaucg      780

agcuugugac caccacaguu agcaacaugg cugaggugag aucuuacugc uacgaagccu      840

ccaucagcga uauggcuucc gacucucggu guccuacgca aggagaggcc uaucuggaca      900

agcagagcga uacccaguac gucugcaaaa gaacacucgu ggaccgcggc uggggcaaug      960

gguguggacu guucggcaag gggucccugg uuaccugcgc caaguuuaca ugcagcaaaa     1020

agaugaccgg aaaaucuauu cagcccgaaa accucgagua ucggaucaug cuguccgugc     1080

acggcagcca acauucuggg augauuguca augaugaaaa cagagcuaag guggagguca     1140

cgccuaauag cccgcgcgcg gaggccacac uuggaggcuu cggcucccug gggcucgacu     1200

gugaaccccg gaccggacug gauuuuucug accuuuacua ucugaccaug aacaauaaac     1260

acuggcucgu gcauaaggag ugguuccacg auaucccucu gcccuggcac gcuggcgccg     1320

acacagggac cccucauugg aacaauaaag aagcccuggu ugaguuuaag gaugcucacg     1380

ccaagagaca gacagugguc gugcucggaa gccaggaagg cgcgguucau acggcucugg     1440

ccggggcccu ugaggcugag auggacggcg ccaaaggacg ccuguucucc ggccaccuca     1500

agugccggcu gaaaauggau aaacuuagac ugaaaggggu gagcuacucu cucuguaccg     1560

cugccuuuac auucaccaag guccccgcgg aaacacugca uggaaccgug acgguggagg     1620

uucaguaugc cggcaccgac gggccgugca aggucccugc ucaaauggcc guggacaugc     1680

agacacugac ccccguggga cgccucauua cagcuaaccc ugucaucacc gaauccacgg     1740

agaacagcaa aaugaugcug gaacuugauc cucccuuugg cgacucuuac aucgugauug     1800

ggguuggcga uaagaaaauc acacaccacu ggcaucgguc cggaagcacc auuggcaagg     1860

ccuucgaggc gacagugaga ggggccaaac gcauggcugu ccugggagac accgccuggg     1920

auuucggcuc cguuggggga guguuuaauu cucucggcaa ggggauccac cagauuuucg     1980

gcgcugccuu uaagagccug uucggaggca uguccugguu uucucaaauc cugaucggga     2040

cccuucucgu guggcuggga cugaacacaa agaauggcag cauuucccuc acgugucugg     2100

cccuuggggg agucaugauc uuccugagca ccgcuguguc ugcggacguu ggcugcuccg     2160

uggauuuuag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6772
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 2 opt

<400>  6772
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc cugggaguga      240

auaaaugcca cguccagauc auggaucucg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcuggaugaa gggguugagc cugacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accauaagaa aggcgaaacc cgcagaagcc      420

ggcgcuccgu gucucuuaga uaucacuaca cacggaagcu gcaaacccgc agccagacau      480

ggcucgaguc cagagaguau aagaaacauc ugauuauggu ugaaaauugg aucuuucgga      540

acccgggcuu cgccauugug agcgucgcga ucaccuggcu gauggggucu cucacguccc      600

agaaggugau uuaccugguu augaucgugc uuauuguccc cgccuacagc aucucuugca      660

ucggaguguc caaucgcgau cugguggagg gcaugagcgg gggaacaugg gucgacguug      720

ugcucgaaca cggcgggugu gugaccgaga uggcucagga uaaaccuaca gucgacauug      780

agcuggugac caugaccguu ucuaacaugg ccgaagugag auccuauugc uacgaggcua      840

gccuuuccga uauggccucu gcgagccggu gucccacaca aggagaaccu ucccuggaca      900

agcagagcga cacgcagucu gucugcaaaa gaacccucgg cgaucgcggc ugggggaaug      960

gauguggcau cuuugggaag ggaucccugg ugacaugcag caaguuuacc ugcuguaaaa     1020

agaugcccgg caaaucuauu cagccugaga accuggaaua ucggaucaug cucccgguuc     1080

acgggagcca acauuccgga augauuguga augacaucgg ccacgagaca gaugagaaca     1140

gagccaaggu cgaagugacc cccaauucuc cucgcgcuga ggccacgcug ggcggguuug     1200

gaagccuugg ccuggacugc gaaccccgga cagggcucga uuucuccgac cuguacuauc     1260

uuaccaugaa caauaaacau uggcuggucc acaaggagug guuucacgau aucccucucc     1320

ccuggcaugc uggagccgac accggcacac cgcacuggaa caauaaagaa gcccuggugg     1380

aguucaagga ugcucaugcg aagagacaga ccguuguggu ccuggggagc caggagggcg     1440

ccgugcacac agcucucgcc ggagcccugg aagcugagau ggacggcgcc aaagggcgcc     1500

uuuucucugg acaucugaag ugucggcuca aaauggauaa gcugagacuu aaaggcguuu     1560

ccuacagccu gugcacggcg gcuuucaccu uuacaaaggu gccugccgaa acccuccacg     1620

ggacagucac cguggaggug cagucugccg gaacggacgg ccccuguaag guuccugcuc     1680

aaauggccgu cgacaugcag acccugacac ccguggggcg ccugauuacc gcuaaccccg     1740

ugaucacaga auccaccgag aacagcaaaa ugaugcucga gcuggauccu ccguucggcg     1800

acuccuauau ugucaucgga gugggcgaua agaaaauuac gcaccauugg caccggucug     1860

ggagcacaau cggaaaggcc uuugaagcga ccguuagagg cgccaaacgc auggcugugc     1920

uuggggacac agccugggau uucggauccg ucggcggggu guucaauucu cugggcaagg     1980

gaauccauca gauuuuuggc gcugccuuca agagccucuu ugggggaaug uccugguuca     2040

gccaaauccu gauuggcacc cugcuuguuu ggcucgggcu gaacaccaaa aauggaucua     2100

ucucccugac augccucgcg cugggcgggg ugaugauuuu ucuuagcacg gccgucucug     2160

cugacguggg cuguuccguu gauuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6773
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 2 opt

<400>  6773
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaacgc ccggaaggag cgcaaaagac ggggcgcuga caccagcauc gggauuaucg      120

gacugcuccu gacaacggcc auggcggcug aaauuacccg cagaggcucc gccuacuaua      180

uguaccuuga ucggucugac gccgggaagg cuaucagcuu cgccacaacc augggaguga      240

auaaaugcca cguccagauc auggaucugg gccauaugug ugacgcuaca auguccuaug      300

agugccccau gcucgaugaa gggguugagc cugacgaugu ggacuguugg ugcaacacca      360

caucuaccug gguggucuac ggaacguguc accauaagaa aggcgaagcc cgcccgagca      420

gacgggcggu gacccugccc ucccacucua cacgcaaacu ucaaaccaga agccagacau      480

ggcuggaguc ccgggaguau accaagcauc ucauuaaggu ugaaaauugg auccugcgca      540

acccuggcuu uacgguggcc gcucuggcca uggcuuggcu ccuggggaau agcacaucuc      600

agagagucau uuaccuugug augauccugc ucauugcccc cgcguauucc auccggugca      660

uuggaguuag caaccgcgau uucguggagg gcaugucugg gggaaccugg gucgacgugg      720

ugcuggaaca cggcgggugu gucacaguua uggcccagga uaagccuacc guggacaucg      780

agcuugugac cacaacgguc uccaauaugg cugaggugag aagcuacugc uacgaagccu      840

cuaucuccga uauggcuagc gacucccggu gucccaccca aggagaggcc uaucuggaca      900

aacagucuga uacacaguac guuugcaaga gaacccucgu ggaccgcggc uggggcaacg      960

gguguggacu guuuggcaaa gggagccugg ucacaugcgc caaguuuacc ugcuccaaga     1020

aaaugacggg aaagagcauu cagccugaaa aucucgagua ucggaucaug cugucugugc     1080

acggcuccca acauagcggg augauuguua acgauaucgg acacgaaaca gacgagaaua     1140

gagcuaaagu ggaggucacc ccgaacucuc cccgcgcgga agccacccuu ggcggcuucg     1200

ggagccuggg acucgauugu gagccucgga caggccugga cuucuccgau cuuuacuauc     1260

ugaccaugaa uaacaagcau uggcucgugc acaaagaaug guuucacgac auuccccugc     1320

cuuggcaugc uggggccgau acaggaacgc cccacuggaa uaacaaggag gcccuggucg     1380

aguucaaaga cgcucaugcc aagagacaga ccgugguugu gcucggcucu caggaagggg     1440

cgguccacac agcucuggcc ggcgcccuug aggcugaaau ggauggagcc aagggccgcc     1500

uguuuagcgg gcaucucaaa ugccggauca agauggacaa acugagacuu aagggagugu     1560

ccuacagccu guguaccgcu gccuucacau uuucuaaagu uccggcggag acccuccacg     1620

gcacggugac cgucgaagug caguaugccg ggcgcgacgg accuugcaag gugcccgcuc     1680

aaauggccgu ugauaugcag acacugaccc cugucggccg gcugaucaca gcuaacccca     1740

uuaucaccga guccacggaa aauagcagaa ugaugcucga gcuggauccc ccuuucgggg     1800

acucuuacau ugugaucggc gugggagaca agaaaauuac acaccauugg caccgcuccg     1860

gcagcaccau cgggaaggcc uuugaggcga caguccgggg agccaaaaga auggcugugc     1920

uuggcgauac cgccugggac uucggguccg uuggaggcgu guucaacucu cuggggaagg     1980

gcguccauca gguguuugga gcugccuuca aaagccucuu uggcgggaug uccugguucu     2040

cucaaauccu gauuggaacc cugcuuguuu ggcucggccu gaauacaaag aacgggagca     2100

ucucccuggc cugucucgcu cugggaggcg cgaugauuuu ucuuagcacg gccgugucug     2160

cugaugucgg gugcuccgug gacuucugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  6774
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 2 opt

<400>  6774
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uauugugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6775
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 2 opt

<400>  6775
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac acaugugaug      240

ccaccaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa cacaaccucc acgugggucg uguaugggac cugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuacac uucccagcca uuccacccgg aaacugcaaa      420

cacgcucuca gaccuggcuc gagagcagag aguacacgaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac aucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

ccugggucga uguggugcug gaacauggag gcugugucac aguuauggcg caggacaagc      720

cuaccgugga uauugagcuu gugaccacaa cggucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acccaagggg      840

aggcuuaccu ggacaagcag ucugacacac aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggucaca ugcgccaaau      960

uugccugcuc caagaaaaug accgggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac acggggcaug     1080

aaacagauga gaaccgggcu aagguggaga uuacccccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcacagga cuggauuuuu     1200

ccgaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacacagg gacgccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagaccguc gugguucucg     1380

gaucucagga gggcgcggug cauacagcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua ccgcugccuu cacauuuacc aagauuccgg     1560

cggagacgcu gcauggaacc gugacaguug aagugcagua cgccggcacc gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacggcuaa ccccguuauu acagagucca ccgaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauacagccu gggacuucgg cuccguuggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacgcuccu gauguggcug ggacucaacg     2040

cuaagaaugg cagcaucucc cugauguguc uugcgcuugg gggagugcuc auuuuccuga     2100

gcaccgccgu gucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6776
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 2 opt

<400>  6776
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauc aagguugaga      480

acuggaucuu ucggaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauucg cugcaucggg gugucuaaca gagacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcacggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucggucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaaagaaca cucgucgauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

uuaugcuguc cguucacgga agccaacauu cuggcaugau cggguacgag acggacgaag     1080

auagagccaa gguggagguc acaccuaaca gcccgcgggc cgaggcuacc cuuggaggcu     1140

ucggcucccu ggggcucgac ugugaaccca gaaccggacu ggauuuuucu gaccuuuauu     1200

accugacaau gaauaacaaa cacuggcucg ugcauaagga gugguuccac gauauuccuc     1260

ugcccuggca cgcgggcgcc gacaccggga caccucauug gaauaacaaa gaagcucugg     1320

ucgaguuuaa ggaugcccac gccaaacgcc agacgguggu ugugcucgga agccaggaag     1380

gcgcugucca uaccgcccug gcgggggcuc uugaggccga gauggacggc gccaagggac     1440

ggcuguucuc cggccaccuc aagugcagac ugaaaaugga uaaacuucgc cugaaggggg     1500

ugagcuauuc ucucuguaca gcugccuuua ccuucacaaa gguucccgcu gaaacccugc     1560

auggaacggu gaccgucgag gugcaguacg ccggcacaga cgggccgugc aaaaucccug     1620

ugcaaauggc gguugacaug cagacccuga cacccgucgg acggcucauu accgccaauc     1680

cugugaucac ggaauccaca gagaacagca agaugaugcu ggaacuugau ccucccuuug     1740

gcgacucuua caucgugauu ggggucggcg auaagaaaau cacccaccac uggcauagau     1800

ccggaagcac aauuggcaag gcuuucgagg ccaccgugcg cggggcuaaa cggauggccg     1860

uucugggaga caccgcgugg gauuucggcu ccgucggggg aguguuuaau ucucucggca     1920

aggggaucca ccagauuuuc ggcgccgcuu uuaaaagccu guucggaggc auguccuggu     1980

uuucucaaau ccugaucggg acacuucucg uguggcuggg acugaacacg aagaacggca     2040

gcauuucccu caccugucug gcccuugggg gaguuaugau cuuccugagc acagcugugu     2100

cugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6777
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 2 opt

<400>  6777
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6778
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 2 opt

<400>  6778
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauc aagguugaga      480

acuggaucuu ucggaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauucg cugcaucggg gugucuaaca gagacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcacggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucggucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaaagaaca cucgucgauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

uuaugcuguc cguucacgga agccaacauu cuggcaugau cgugaacgac acgggguacg     1080

agacagauga aaauagagcc aaggucgagg ugaccccuaa cagcccgcgg gccgaggcua     1140

cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaacagga cuggauuuuu     1200

cugaccuuua uuaccugacc augaauaaca aacacuggcu cguccauaag gagugguucc     1260

acgauauucc ucugcccugg cacgcgggcg ccgacacagg gacgccucau uggaauaaca     1320

aagaagcucu gguggaguuu aaggaugccc acgccaaacg ccagaccguu gugguccucg     1380

gaagccagga aggcgcugug cauacagccc uggcgggggc ucuugaggcc gagauggacg     1440

gcgccaaggg aaagcuguuc uccggccacc ucaaaugccg gcugaagaug gauaaacuua     1500

gacugaaggg gguuagcuau ucucucugua ccgcugccuu uacauucacc aaagugcccg     1560

cugaaacgcu gcauggaacc gucacagugg aggugcagua cgccggcacc gacgggccgu     1620

gcaagauccc uguucaaaug gcggucgaca ugcagacacu gacccccgug ggacgccuca     1680

uuacggccaa uccugugauc acagaaucca ccgagaacag caagaugaug cuggaacuug     1740

auccucccuu uggcgacucu uacaucguca uugggguggg cgauaaaaag aucacacacc     1800

acuggcaucg guccggaagc accauuggca aggcuuucga ggccaccguu agaggggcua     1860

aacgcauggc cgugcuggga gacacagcgu gggauuucgg cuccgucggg ggaguguuua     1920

acucucucgg caaagggauc caccagauuu ucggcgccgc uuuuaagagc cuguucggag     1980

gcauguccug guuuucucaa auccugaucg ggacgcuucu cguuuggcug ggacugaaua     2040

ccaagaacgg cagcauuucc cucacauguc uggcccuugg gggagugaug aucuuccuga     2100

gcaccgcugu cucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6779
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 2 opt

<400>  6779
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucacaga aagaaaggag      360

aggcccggcg cagcagacgg gcugugaccc uuccguccca uucuacacgc aagcugcaaa      420

ccagaagcca gacauggcuc gaaucccggg aguauaccaa acaccugauc aagguugaga      480

acuggaucuu ucgcaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauuag augcaucggg gugucuaacc gggacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcauggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucgcucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc uagauguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaacggaca cucgucgauc      900

gcggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauauagaa     1020

uuaugcuguc cguucacgga agccaacacu cuggcaugau cgugaacgac acgggguacg     1080

agacagauga aaaucgggcc aaggucgagg ugaccccuaa cagcccgaga gccgaggcua     1140

cccuuggagg cuucggcucc cuggggcucg acugugaacc ccgcacagga cuggauuuuu     1200

cugaccuuua uuaccugacc augaauaaca aacauuggcu cguccacaag gagugguucc     1260

augauauucc ucugcccugg cacgcgggcg ccgacacagg gacgccucac uggaauaaca     1320

aagaagcucu gguggaguuu aaggaugccc augccaaacg gcagaccguu gugguccucg     1380

gaagccagga aggcgcugug cacacagccc uggcgggggc ucuugaggcc gagauggacg     1440

gcgccaaggg aaagcuguuc uccggccauc ucaaaugcag acugaagaug gauaaacuuc     1500

gccugaaggg gguuagcuau ucucucugua ccgcugccuu uacauucacc aaagugcccg     1560

cugaaacgcu gcacggaacc gucacagugg aggugcagua cgccggcacc gacgggccgu     1620

gcaagauccc uguucaaaug gcggucgaca ugcagacacu gacccccgug ggacggcuca     1680

uuacggccaa uccugugauc acagaaucca ccgagaacag caagaugaug cuggaacuug     1740

auccucccuu uggcgacucu uacaucguca uugggguggg cgauaaaaag aucacacauc     1800

acuggcacag auccggaagc accauuggca aggcuuucga ggccaccguu cgcggggcua     1860

aacggauggc cgugcuggga gacacagcgu gggauuucgg cuccgucggg ggaguguuua     1920

acucucucgg caaagggauc caucagauuu ucggcgccgc uuuuaagagc cuguucggag     1980

gcauguccug guuuucucaa auccugaucg ggacgcuucu cguuuggcug ggacugaaua     2040

ccaagaacgg cagcauuucc cucacauguc uggcccuugg gggagugaug aucuuccuga     2100

gcaccgcugu cucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6780
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 2 opt

<400>  6780
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauc aagguugaga      480

acuggaucuu ucggaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauucg cugcaucggg gugucuaaca gagacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcacggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucggucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaaagaaca cucgucgauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

uuaugcuguc cguucacgga agccaacauu cuggcaugac ggugaacgac aucggguacg     1080

agacagauga aaauagagcc aaggucgagg ugaccccuaa cagcccgcgg gccgaggcua     1140

cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaacagga cuggauuuuu     1200

cugaccuuua uuaccugacc augaauaaca aacacuggcu cguccauaag gagugguucc     1260

acgauauucc ucugcccugg cacgcgggcg ccgacacagg gacgccucau uggaauaaca     1320

aagaagcucu gguggaguuu aaggaugccc acgccaaacg ccagaccguu gugguccucg     1380

gaagccagga aggcgcugug cauacagccc uggcgggggc ucuugaggcc gagauggacg     1440

gcgccaaggg aaagcuguuc uccggccacc ucaaaugccg gcugaagaug gauaaacuua     1500

gacugaaggg gguuagcuau ucucucugua ccgcugccuu uacauucacc aaagugcccg     1560

cugaaacgcu gcauggaacc gucacagugg aggugcagua cgccggcacc gacgggccgu     1620

gcaagauccc uguucaaaug gcggucgaca ugcagacacu gacccccgug ggacgccuca     1680

uuacggccaa uccugugauc acagaaucca ccgagaacag caagaugaug cuggaacuug     1740

auccucccuu uggcgacucu uacaucguca uugggguggg cgauaaaaag aucacacacc     1800

acuggcaucg guccggaagc accauuggca aggcuuucga ggccaccguu agaggggcua     1860

aacgcauggc cgugcuggga gacacagcgu gggauuucgg cuccgucggg ggaguguuua     1920

acucucucgg caaagggauc caccagauuu ucggcgccgc uuuuaagagc cuguucggag     1980

gcauguccug guuuucucaa auccugaucg ggacgcuucu cguuuggcug ggacugaaua     2040

ccaagaacgg cagcauuucc cucacauguc uggcccuugg gggagugaug aucuuccuga     2100

gcaccgcugu cucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6781
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 2 opt

<400>  6781
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccugacaa cggccauggc ugccgagauc accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauu agcuucgcca      180

caacccuggg ggucaacaaa ugccacguuc agaucaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ggaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcuguuaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauu aagguggaga      480

acuggaucuu ucggaauccc ggcuucacgc uggucgccgu ggcgaucgcc uggcuccugg      540

gcagcucuac aucccagaag guuauuuacc uugugaugau ccugcucauu gcuccugccu      600

auagcauccg cugcauuggg gucucuaaca gagacuuugu ggaaggaaug uccggcggga      660

ccugggugga ugucguucug gagcacggag gcugugugac agugauggcu caggacaaac      720

ccaccgucga uaucgaacuu gugaccacaa cgguuagcaa uauggccgag gugcggucuu      780

acugcuacga ggcguccauu agcgacaugg ccuccgauuc ucgcuguccu acccaagggg      840

aggcuuaucu ggacaagcag agcgacacac aguacgucug caaaagaacc cucguggauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccugguuaca ugcgccaaau      960

uuaccugcag caagaagaug acggggaaau cuauccagcc cgaaaaucuc gaauaucgca     1020

ucaugcuguc cgugcacgga agccaacauu cuggcaugau ugucaacgac gagaauagag     1080

cuaaggugga agucacaccu aacagcccgc gggccgaggc cacccuuggg ggauucggcu     1140

cccugggccu cgauugugag cccagaaccg ggcuggacuu uucugaucuu uacuaucuga     1200

caaugaauaa caaacacugg cucgugcaua aggaaugguu ccacgacauc ccucugcccu     1260

ggcacgcugg agcggauacc ggcacaccuc auuggaauaa caaagaggcc cugguugaau     1320

uuaaggacgc ucacgccaaa cgccagacgg uggucgugcu cgggagccag gagggagccg     1380

uucauaccgc ucuggccggc gcgcuugaag cugagaugga uggggccaag ggccggcugu     1440

ucuccggaca ccucaagugc agacugaaaa uggacaaacu ucgccugaag ggcgugagcu     1500

acucucucug uacagccgcu uuuaccuuca caaagguccc cgccgagacc cugcauggga     1560

cggugaccgu ggaaguucag uaugcuggaa cagauggccc gugcaaaguc ccugcccaaa     1620

uggcggugga caugcagacc cugacacccg uggggcggcu cauuaccgcc aauccuguca     1680

ucacggaguc cacagaaaac agcaagauga ugcuggagcu ugacccuccc uuuggagauu     1740

cuuacauugu gaucggcguu ggggacaaga aaaucaccca ccacuggcau agauccggca     1800

gcacaauugg aaaggcuuuc gaagccaccg ugcgcggcgc uaaacggaug gccguccugg     1860

gggauaccgc gugggacuuc ggauccgugg gcgggguuuu uaacucucuc ggaaagggca     1920

uccaccagau uuucggggcc gcuuuuaaaa gccuguucgg cggaaugucc ugguuuucuc     1980

aaauccugau uggcacacuu cucguguggc uggggcugaa uacgaagaac ggaagcaucu     2040

cccucaccug ucuggcccuu ggcgggguca ugaucuuccu gagcacagcu gugucugccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6782
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 2 opt

<400>  6782
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauc aagguugaga      480

acuggaucuu ucggaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauucg cugcaucggg gugucuaaca gagacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcacggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucggucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaaagaaca cucgucgauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

uuaugcuguc cguucacgga agccaacauu cuggcaugau cggguacgag acggacgaaa     1080

acagagccaa gguggagguc acaccuaaua gcccgcgggc cgaggcuacc cuuggaggcu     1140

ucggcucccu ggggcucgau ugugaaccca gaaccggacu ggacuuuucu gaucuuuauu     1200

accugacaau gaacaauaaa cacuggcucg ugcauaagga gugguuccac gacauuccuc     1260

ugcccuggca cgcgggcgcc gauaccggga caccucauug gaacaauaaa gaagcucugg     1320

ucgaguuuaa ggacgcccac gccaaacgcc agacgguggu ugugcucgga agccaggaag     1380

gcgcugucca uaccgcccug gcgggggcuc uugaggccga gauggauggc gccaagggaa     1440

agcuguucuc cggccaccuc aaaugccggc ugaagaugga caaacuuaga cugaaggggg     1500

ugagcuauuc ucucuguaca gcugccuuua ccuucacaaa aguucccgcu gaaacccugc     1560

auggaacggu gaccgucgag gugcaguacg ccggcacaga ugggccgugc aagaucccug     1620

ugcaaauggc gguugacaug cagacccuga cacccgucgg acgccucauu accgccaacc     1680

cugugaucac ggaauccaca gagaauagca agaugaugcu ggaacuugac ccucccuuug     1740

gcgauucuua caucgugauu ggggucggcg acaaaaagau cacccaccac uggcaucggu     1800

ccggaagcac aauuggcaag gcuuucgagg ccaccgugag aggggcuaaa cgcauggccg     1860

uucugggaga uaccgcgugg gacuucggcu ccgucggggg aguguuuaac ucucucggca     1920

aagggaucca ccagauuuuc ggcgccgcuu uuaagagccu guucggaggc auguccuggu     1980

uuucucaaau ccugaucggg acacuucucg uguggcuggg acugaacacg aagaauggca     2040

gcauuucccu caccugucug gcccuugggg gaguuaugau cuuccugagc acagcugugu     2100

cugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6783
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 2 opt

<400>  6783
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg ccgcgggauu caccagaucu ucggcgccgc cuuuaagagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaaaaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6784
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 2 opt

<400>  6784
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggccuggcuc ucgccgcggc cgcuaucgcc uggcugcuug      540

gcuccagcac gucucagaaa gucaucuauc uggugaugau ucuccugauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuucgu ggagggaaug ucuggcggga      660

caugggucga uguggugcuu gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcug gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu cgacaagcag ucugacaccc aguauguuug caaacggaca cugguggauc      900

gcggaugggg caauggcugu gggcuguuug gaaagggcag ccucgugacc ugcgccaaau      960

ucgccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccug gaguacagaa     1020

ucaugcuuuc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccugggagg cuuuggcagc cucgggcugg acugugagcc ucgcaccgga cuugauuucu     1200

ccgaccugua uuaccucaca augaacaaua aacacuggcu gguccauaag gaaugguuuc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu cguggaguuc aaggaugcuc acgccaaacg gcagacgguc gugguucugg     1380

gaucucagga aggcgcggug cauaccgcuc uugccggggc ccuggaggcu gaaauggacg     1440

gcgccaaggg aagacucagc uccggccacc ugaaaugccg ccuuaagaug gauaagcugc     1500

ggcucaaagg ggucagcuau ucucugugua cagcugccuu uaccuucaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu cacaccugug ggaagacuga     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuugagcugg     1740

aucccccuuu uggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuucga agcgaccgug agaggggcca     1860

aacggauggc uguccucgga gauaccgccu gggacuuugg cuccgucggg ggagcucuua     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuucaaaagc cuguuuggag     1980

gcauguccug guucucucaa auucucaucg ggacacugcu gauguggcuc ggacugaaca     2040

cgaagaaugg cagcaucucc cuuauguguc uggcucucgg gggaguucug auuuuucuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6785
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 2 opt

<400>  6785
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acugguaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauu caucagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6786
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 2 opt

<400>  6786
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca cccagacacu gaccccugug ggaagacuca     1680

ucacggcuaa ccccguuauu acagagucca ccgaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauacagccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacgcuccu gauguggcug ggacucaaca     2040

ccaagaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcacagcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6787
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 2 opt

<400>  6787
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag auccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6788
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 2 opt

<400>  6788
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acaggaaaga gcauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacggg ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucucccaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccu ggcucaaaug gccguggaca ugcagacccu cacacccgug ggaagacuga     1680

ucaccgcuaa cccuguuauu acggagucca cagaaaacag caagaugaug cuugagcugg     1740

auccgcccuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccucgga gauaccgccu gggacuucgg cuccgucggg ggagcucuua     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuuuucggag     1980

gcauguccug guuuucucaa auucucaucg ggacacugcu gauguggcuc ggacugaaca     2040

cgaagaaugg cagcaucucc cuuauguguc uggcucucgg gggaguucug auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6789
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 2 opt

<400>  6789
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuacccugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa cacaaccucc acgugggucg uguaugggac cugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuacac uucccagcca uuccacccgg aaacugcaaa      420

cacgcucuca gaccuggcuc gagagcagag aguacacgaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac aucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

ccugggucga uguggugcug gaacauggag gcugugucac aguuauggcg caggacaagc      720

cuaccgugga uauugagcuu gugaccacaa cggucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acccaagggg      840

aggcuuaccu ggacaagcag ucugacacac aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggucaca ugcgccaaau      960

uugccugcuc caagaaaaug accgggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac acggggcaug     1080

aaacagauga gaaccgggcu aagguggaga uuacccccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcacagga cuggauuuuu     1200

ccgaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacacagg gacgccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagaccguc gugguucucg     1380

gaucucagga gggcgcggug cauacagcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua ccgcugccuu cacauuuacc aagauuccgg     1560

cggagacgcu gcauggaacc gugacaguug aagugcagua cgccggcacc gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacggcuaa ccccguuauu acagagucca ccgaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauacagccu gggacuucgg cuccguuggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacgcuccu gauguggcug ggacucaaca     2040

ccaagaaugg cagcaucucc cugauguguc uugcucuugg gggagugcuc auuuuccuga     2100

gcacagcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6790
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 2 opt

<400>  6790
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcaccguggu cucugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6791
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 2 opt

<400>  6791
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc gcuugggucg uguaugggac cugucaucac aaaaagggag      360

aagccagacg gucucgcaga gcuguuacgc uucccagcca uuccacccgg aaacugcaaa      420

cacgcccuca gaccuggcuc gagucuagag aguacacaaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccc ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg      540

gcagcuccac cagccagaaa gucaucuauc uugugaugau ucugcucauc gccccugcgu      600

acucuauuag augcaucggg guuuccaauc gggacuuugu ggagggaaug agcggcggga      660

cgugggucga uguggugcug gaacauggag gcugugucac aguuauggcc caggacaagc      720

ccaccgugga uauugagcuu gugacaacca ccgucucuaa cauggcugag gugcgcuccu      780

acugcuauga agccagcauc ucugacaugg cuuccgauag cagauguccu acacaagggg      840

aggccuaccu ggacaagcag uccgacacgc aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcuc ucuggucaca ugcgccaaau      960

uugcuugcag caagaaaaug accgggaagu ccauucagcc ggaaaaccuc gaguacagaa     1020

ucaugcugag cgugcacgga ucucaacacu ccggcaugau cguuaaugac acagggcaug     1080

aaaccgauga gaaccgggcg aagguggaga uuacgcccaa uagcccuaga gccgaagcua     1140

cacuuggagg cuucggcucu cuggggcucg acugugagcc ccgcaccgga cuggauuuua     1200

gcgaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ucugcccugg cacgccggcg ccgacacagg gaccccgcau uggaacaaua     1320

aagaggcucu gguggaauuu aaggaugccc acgcgaaacg gcagacaguc gugguucucg     1380

gaucccagga gggcgcugug cauacggccc uggccggggc ucuugaggcc gaaauggacg     1440

gcgcuaaggg aagacugucu agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucuccuau agccucugua ccgccgcguu cacauuuacc aagauuccug     1560

ccgagacacu gcauggaacc gugacgguug aagugcagua cgcuggcacc gacgggcccu     1620

gcaaaguccc ugcccaaaug gcuguggaca ugcagacacu gacccccgug ggaagacuca     1680

ucacagccaa ccccguuauu accgagucua cggaaaacuc caagaugaug cuggagcuug     1740

auccuccguu cggcgacagc uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cucuggaucc accaucggca aggcguuuga agccacagug agaggggcua     1860

aacggauggc cguccuggga gauaccgcuu gggacuucgg cagcguuggg ggagcccuca     1920

auucccuggg caaggggauu caccagaucu ucggcgccgc uuuuaaaucu cuguucggag     1980

gcaugagcug guuuucccaa auucuuaucg ggacccuccu gauguggcug ggacucaaca     2040

caaagaaugg cucuaucagc cugauguguc uugcgcuugg gggagugcuc auuuuccugu     2100

ccacggccgu gagcgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6792
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 2 opt

<400>  6792
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucuaaaaag gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaggggg      840

aggcuuaccu ggacaaacaa ucugacaccc aguauguuug caagcggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaaaggcag ccuggugacc ugcgccaagu      960

uugccugcuc caaaaagaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccagcaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aaaguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua agcacuggcu cguccauaaa gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aggaggcccu gguggaauuu aaagaugcuc acgccaagcg gcaaacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaagg aagacugagc uccggccacc ucaagugccg ccugaagaug gauaaacuuc     1500

ggcugaaggg ggucagcuau ucucucugua cagcugccuu caccuuuaca aaaauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaagguccc cgcucagaug gccguggaca ugcaaacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caaaaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaagaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aagccuuuga agcgaccgug agaggggcca     1860

agcggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaagggauu caccagaucu ucggcgccgc cuuuaagagc cuguucggag     1980

gcauguccug guuuucucag auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaaaaaugg cagcaucucc cugauguguc uugcucucgg gggaguucug auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6793
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 2 opt

<400>  6793
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gagacagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acucuauucg gugcaucggg guuuccaauc gcgacuuugu ggagggaaug agcggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucucuaa cauggccgag gugagauccu      780

acugcuauga agcuagcauc ucugacaugg ccuccgauag ccgguguccc acgcaagggg      840

aggcuuaccu ggacaagcag uccgacaccc aguauguuug caaacgcaca cucguggaua      900

gaggaugggg caauggcugu gggcuguucg gaaagggcuc ucuggugacc ugcgccaaau      960

uugccugcag caagaaaaug acagggaagu ccauucagcc ugaaaaccuc gaguaccgga     1020

ucaugcugag cgugcacgga ucucaacacu ccggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaacagagcu aagguggaga uuacaccgaa uagcccccgc gcggaagcca     1140

cccuuggagg cuucggcucu cuggggcucg acugugagcc ucggaccgga cuggauuuua     1200

gcgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaaag acagacgguc gugguucucg     1380

gaucccagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg acgccugucu agcggccacc ucaaaugccg gcugaagaug gauaagcuga     1500

gacugaaagg ggucuccuau agccucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggacgccuca     1680

ucaccgcuaa ccccguuauu acggagucua cagaaaacuc caagaugaug cuggagcuug     1740

aucccccuuu cggcgacagc uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg gucuggaucc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacgcauggc uguccuggga gauaccgccu gggacuucgg cagcgucggg ggagcucuca     1920

auucccuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaucu cuguucggag     1980

gcaugagcug guuuucccaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cucuaucagc cugauguguc uugcucuugg gggaguucuc auuuuccugu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6794
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 2 opt

<400>  6794
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaacccagga gggcgcggug cauacagcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucu agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucuccuau agccucugua ccgcugccuu cacauuuacc aagauuccgg     1560

cggagacgcu gcauggaacc gugacaguug aagugcagua cgccggcacc gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacggcuaa ccccguuauu acagagucua ccgaaaacuc caagaugaug cuggagcuug     1740

aucccccuuu cggcgacagc uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cucuggaucc accaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauacagccu gggacuucgg cagcgucggg ggagcucuca     1920

auucccuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaucu cuguucggag     1980

gcaugagcug guuuucccaa auucuuaucg ggacgcuccu gauguggcug ggacucaaca     2040

ccaagaaugg cucuaucagc cugauguguc uugcucuugg gggaguucuc auuuuccugu     2100

ccacagcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6795
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 2 opt

<400>  6795
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acucccugag augcauuggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcuu gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uaucgagcug gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu cgacaagcag ucugacaccc aguauguuug caaacggaca cugguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccucgugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccug gaguacagaa     1020

uuaugcuuuc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga ucacacccaa uucuccgaga gcggaagcca     1140

cccugggagg cuucggcagc cucgggcugg acugugagcc ucgcaccgga cuugauuuuu     1200

ccgaccugua uuaccucaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu cguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucugg     1380

gaucucagga gggcgcggug cauaccgcuc uugccggggc ccuggaggcu gaaauggacg     1440

gcgccaaggg aagacucagc uccggccacc ugaaaugccg ccuuaagaug gauaagcugc     1500

ggcucaaagg ggucagcuau ucucugugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu cacaccugug ggaagacuga     1680

uuaccgcuaa ccccguuauc acggagucca cagaaaacag caagaugaug cuugagcugg     1740

aucccccuuu cggcgacucu uacauuguca ucgggguggg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccucgga gauaccgccu gggacuucgg cuccgucggg ggagcucuua     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccucauug ggacacugcu gauguggcuc ggacugaaca     2040

cgaagaaugg cagcaucucc cuuauguguc uggcucucgg gggaguucug aucuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6796
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 2 opt

<400>  6796
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggauugaaa      480

auuggaucuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gugauuuauc uugucaugau ccugcucauu gcucccgccu      600

acuccaucag augcauuggg gugagcaauc gggacuuugu ugagggaaug ucuggcggga      660

caugggugga ugucgugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucucagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcucuugg gggagugcuc aucuuccuga     2100

gcaccgcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6797
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 2 opt

<400>  6797
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauucu gcgcaacccu ggcuuugcuc ucgccgcggc cgcuaucgcc uggcugcuug      540

gcuccagcac gucucagaaa gucaucuauc uggugaugau ucuccugauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuucgu ggagggaaug ucuggcggga      660

caugggucga uguggugcuu gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcug gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu cgacaagcag ucugacaccc aguauguuug caaacggaca cugguggauc      900

gcggaugggg caauggcugu gggcuguuug gaaagggcag ccucgugacc ugcgccaaau      960

ucgccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccug gaguacagaa     1020

ucaugcuuuc uguccacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccugggagg cuuuggcagc cucgggcugg acugugagcc ucgcaccgga cuugauuucu     1200

ccgaccugua uuaccucaca augaacaaua aacacuggcu gguccauaag gaaugguuuc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu cguggaguuc aaggaugcuc acgccaaacg gcagacgguc gugguucugg     1380

gaucucagga aggcgcggug cauaccgcuc uugccggggc ccuggaggcu gaaauggacg     1440

gcgccaaggg aagacucagc uccggccacc ugaaaugccg ccuuaagaug gauaagcugc     1500

ggcucaaagg ggucagcuau ucucugugua cagcugccuu uaccuucaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu cacaccugug ggaagacuga     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuugagcugg     1740

aucccccuuu uggcgacucu uacaucguca ucgggauugg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuucga agcgaccgug agaggggcca     1860

aacggauggc ugugcucgga gauaccgccu gggacuuugg cuccgucggg ggagcucuua     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuucaaaagc cuguuuggag     1980

gcauguccug guucucucaa auccucaucg ggacacugcu gauguggcuc ggacugaaca     2040

cgaagaaugg cagcauuucc cuuauguguc uggcucucgg gggagugcug aucuuucuga     2100

gcaccgcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6798
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 2 opt

<400>  6798
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu guguccacca ccgucagcaa cauggccgag gugcgcucuu      780

acugcuauga agcuacaauc uccgacauug ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau uguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga ucacaccgaa uagccccaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacggcg gucgugcucg     1380

gaucucagga gggcgcuguu cauaccgccc uggccggggc ucuugaggcc gaaauggacg     1440

gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagccgcguu caccuuuaca aagaucccgg     1560

ccgagacccu gcauggaacg gucaccgugg aaguucagua cgcuggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcugucgaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgccaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguua uuggggucgg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggcguuuga agccaccgug agaggggcua     1860

aacggauggc cgugcuggga gauaccgcuu gggacuucgg cuccgucggg ggagcccuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcauuucc cugauguguc uugcgcuugg gggagugcuc aucuuccuga     2100

gcaccgccgu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6799
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 2 opt

<400>  6799
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aacugcagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu cacaccugug ggaagacuga     1680

ucaccgcuaa ccccgugauu acggagucca cagaaaacag caagaugaug cuugagcugg     1740

aucccccuuu cggcgacucu uacaucguua ucggggucgg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcucgga gauaccgccu gggacuucgg cuccgucggg ggagcucuua     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuuuucggag     1980

gcauguccug guuuucucaa auucucaucg ggacacugcu gauguggcuc ggacugaaca     2040

cgaagaaugg cagcaucucc cuuauguguc uggcucucgg gggagugcug auuuuccuga     2100

gcaccgcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6800
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 2 opt

<400>  6800
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca ucgaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca uugggguggg cgagaaaaag aucacacacc     1800

acuggcaucg cuccggaagc accauuggca aggccuuuga agcgacagug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacccuccu gauguggcug ggacucaaca     2040

caaagaaugg cagcauuucc cugauguguc uugcucuugg gggaguucuc aucuuccuga     2100

gcacggcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6801
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 2 opt

<400>  6801
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgucaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaaacuuc     1500

ggcugaaggg ggugagcuau ucucucugua cagccgcuuu caccuuuaca aagauuccgg     1560

ccgagacccu gcauggaacg guuaccgugg aaguccagua cgcgggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcuguggaca ugcagacccu gacaccuguu ggaagacuca     1680

ucaccgccaa ccccgucauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguga ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggcuuuuga agccaccguc agaggggcga     1860

aacggauggc cgugcuggga gauaccgcuu gggacuucgg cuccguuggg ggagcccuca     1920

auucucuggg caaggggauu caccagaucu ucggcgcugc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc uucauguguc uggcccuugg gggagugcug auuuuucuca     2100

gcaccgcugu cucugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6802
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 2 opt

<400>  6802
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aaaacugagc uccggccacc ucaagugcag acugaagaug gauaaacuuc     1500

gccugaaggg ggucagcuau ucucucugua cagcugccuu caccuuuaca aaaauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaagguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggacggcuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caaaaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaagaag auuacccacc     1800

acuggcauag auccggaagc acaaucggca aagccuuuga agcgaccgug cgcggggcca     1860

agcggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaagggauu caccagaucu ucggcgccgc cuuuaagagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaaaaaugg cagcaucucc cugauguguc uugcucucgg gggaguucug auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6803
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 2 opt

<400>  6803
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca aggaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaacaaa ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa uaccacaucc accugggucg uguaugggac gugucaucac aagaaaggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aagcugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa acaccugauc cggguggaaa      480

acuggauuuu ucgcaauccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaag gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaacc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa uauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaaacag ucugacaccc aguauguuug caagcggaca cucguggauc      900

gcggaugggg caacggcugu gggcuguucg gaaaaggcag ccuggugacc ugcgccaagu      960

uugccugcuc caaaaagaug acagggaagu cuauucagcc ugaaaaucuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaacgac accgggcaug     1080

aaacggauga gaaucgggcu aaaguggaga uuacaccgaa cucucccaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaauaaca agcacuggcu cguccauaaa gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaauaaca     1320

aggaggcccu gguggaauuu aaagaugcuc acgccaagcg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaagg aagacugagc uccggccacc ucaagugccg ccugaagaug gauaaacuuc     1500

ggcugaaggg ggucagcuau ucucucugua cagcugccuu caccuuuaca aaaauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaagguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ucccguuauu acggagucca cagaaaacag caaaaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaagaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aagccuuuga agcgaccgug agaggggcca     1860

agcggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

acucucuggg caaagggauu caccagaucu ucggcgccgc cuuuaagagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaua     2040

cgaaaaacgg cagcaucucc cugauguguc uugcucucgg gggaguucug auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6804
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 2 opt

<400>  6804
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acaggaaaga gcauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacggg ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucucccaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaggcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaagcu gagauggaug     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gacaaacuuc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggaaacccu gcauggaacg gugaccguug aggugcagua cgccggcaca gaugggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggaaucca cagagaacag caagaugaug cuggagcuug     1740

acccgccuuu cggcgauucu uacaucguca ucgggguggg cgaaaagaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga ggcgaccgug agaggggcca     1860

aacggauggc uguccuggga gacaccgccu gggauuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcucucgg gggaguucug auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6805
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 2 opt

<400>  6805
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acaggaaaga gcauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacggg ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucucccaga gcggaagccg     1140

cucuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgccggcg ccgacacagg gaccccgcau uggaacaaua     1320

aagaggcucu gguggaauuu aaggaugccc acgcgaaacg gcagacaguc gugguucucg     1380

gaucucagga gggcgcugug cauacggccc uggccggggc ucuugaggcc gaaauggacg     1440

gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua ccgccgcguu cacauuuacc aagauuccgg     1560

ccgagacacu gcauggaacc gugacgguug aagugcagua cgcuggcacc gacgggccuu     1620

gcaaaguccc cgcccaaaug gcuguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacagccaa ccccguuauu accgagucca cggaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggcguuuga agccacagug agaggggcua     1860

aacggauggc cguccuggga gauaccgcuu gggacuucgg cuccgucggg ggagcccuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa acccuuauug ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcgcuugg gggaguucuc aucuuccuga     2100

gcaccgccgu gucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6806
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 2 opt

<400>  6806
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcgcu gacgggccuu     1620

gcaaaguccc cgcccaaaug gcuguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacagccaa ccccguuauu accgagucca cggaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggcguuuga agccacagug agaggggcua     1860

aacggauggc cguccuggga gauaccgcuu gggacuucgg cuccgucggg ggagcccuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacccuccu gauguggcug ggacucaaca     2040

caaagaaugg cagcaucucc cugauguguc uugcgcuugg gggaguucuc auuuuccuga     2100

gcacggccgu gucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6807
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 2 opt

<400>  6807
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc cccaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucuggccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcuu gaacauggag gcugugucac cguuauggcg caggacaagc      720

ccacagugga uauugagcug gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccu acgcaagggg      840

aggcuuaccu cgacaagcag ucugacaccc aguauguuug caaacggaca cugguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccucgugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc cgaaaaccug gaguacagaa     1020

ucaugcuuuc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacaccuaa uucuccgaga gcggaagcca     1140

cccugggagg cuucggcagc cucgggcugg acugugagcc ccgcaccgga cuugauuuuu     1200

ccgaccugua uuaccucaca augaacaaua aacacuggcu gguccauaag gaaugguucc     1260

acgauauccc ucugcccugg cacgcuggcg ccgacaccgg gacaccucau uggaacaaua     1320

aagaggcccu cguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucugg     1380

gaucucagga gggcgcggug cauaccgcuc uugccggggc ccuggaggcu gaaauggacg     1440

gcgccaaggg aagacucagc uccggccacc ugaaaugccg ccuuaagaug gauaagcugc     1500

ggcucaaagg ggucagcuau ucucugugua cagcugccuu caccuuuaca aagauucccg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccgu     1620

gcaaaguccc ugcucaaaug gccguggaca ugcagacccu cacacccgug ggaagacuga     1680

ucaccgcuaa cccuguuauu acggagucca cagaaaacag caagaugaug cuugagcugg     1740

auccucccuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccucgga gauaccgccu gggacuucgg cuccgucggg ggagcucuua     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucucaucg ggacacugcu gauguggcuc ggacugaaca     2040

cgaagaaugg cagcaucucc cuuauguguc uggcucucgg gggaguucug auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6808
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 2 opt

<400>  6808
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg uuagcgauuc cagauguccc acgcaagggg      840

aggccuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucgucgauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgcuaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguucacgga ucccaacaca gcggcaugau cgugaaugac accgggcaug     1080

aaacggauga gaaccgggcc aaggucgaga uuacacccaa uucuccgaga gcugaagcga     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgccggcg cugacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugccc acgcuaaacg gcagacggug guugugcucg     1380

gaucucagga gggcgccguc cauaccgcgc uggcuggggc ccuugaggcc gaaauggacg     1440

gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugagcuau ucucucugua cagccgcuuu caccuuuaca aagauuccgg     1560

ccgagacccu gcauggaacg guuaccgugg aaguccagua cgcgggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcuguggaca ugcagacccu gacaccuguu ggaagacuca     1680

ucaccgccaa ccccgucauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguga ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggcuuuuga agccaccguc agaggggcga     1860

aacggauggc cgugcuggga gauaccgcuu gggacuucgg cuccguuggg ggagcccuca     1920

auucucuggg caaggggauu caccagaucu ucggcgcugc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcccuugg gggagugcuc auuuuccuga     2100

gcaccgcugu cucugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6809
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 2 opt

<400>  6809
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg auuagcaauc gggacuuugu ugagggaaug ucuggcggga      660

caugggugga ugucgugcug gaacauggag gcugugugac cgucauggcg caggacaagc      720

cuacaguuga uaucgagcuu gugaccacca caguguccaa cauggccgag guccgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugau ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguugaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcauu cuggggcucg acugugagcc ucgcaccgga cuggauuuua     1200

gcgaccuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagacggug gucgugcucg     1380

gaucccagga gggcgcgguu cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucu agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg gguguccuau agccucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gucaccgugg aaguucagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccgugauc acggagucua cagaaaacuc caagaugaug cuggagcuug     1740

aucccccuuu cggcgacagc uacauuguua ucggggucgg cgagaaaaag auuacccacc     1800

acuggcaucg cucuggaucc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cagcgucggg ggagcucuca     1920

auucccuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaucu cuguucggag     1980

gcaugagcug guuuucccaa auccuuauug ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cucuaucagc cugauguguc uugcucuugg gggagugcuc auuuuccugu     2100

ccaccgcggu uagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6810
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 2 opt

<400>  6810
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac acaugugaug      240

ccaccaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa cacaaccucc acgugggucg uguaugggac cugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuacac uucccagcca uuccauccgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauu cggguggaaa      480

auuggaucuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucauuuauc uugugaugau ccugcucauu gcucccgccu      600

acuccaucag augcauuggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uaucgagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau uguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccguuauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca uugggguggg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuaucg ggacacuccu gauguggcug ggacucaacg     2040

cuaagaaugg cagcauuucc cugauguguc uugcgcuugg gggaguucuc aucuuccuga     2100

gcacggccgu gucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6811
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 2 opt

<400>  6811
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcucaaag aucucggcgc gccguuaccc uucccagcca uuccacaaga aaacugcaga      420

cccggucuca gacauggcuc gagagccgcg aguacaccaa gcaccugauc agaguggaaa      480

auuggauuuu ucggaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauucg cugcaucggg guuagcaaua gagacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caagacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcggagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc ccgcuguccc acgcaggggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaaagaaca cucguggauc      900

ggggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucaacc ugaaaaccuc gaguaccgca     1020

ucaugcuguc ugugcacgga ucccagcaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaacagagcu aagguggaga uuacacccaa uucuccgcgg gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc uagaaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg ccagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg acggcugagc uccggccacc ucaaaugcag acugaagaug gauaagcugc     1500

gccugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcaaua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucagaug gccguggaca ugcagacccu gacaccugug ggacggcuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcauag auccggaagc acaaucggca aggccuuuga agcgaccgug cgcggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauu caccaaaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucag auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6812
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 2 opt

<400>  6812
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaag      420

cucgcucuca gaccuggcuc gagagcagag aguacacaaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg      540

gcuccagcac cucucagaaa gucaucuauc uugugaugau ucugcucauc gcccccgcgu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

cgugggucga uguggugcug gaacauggag gcugugucac aguuauggcc caggacaagc      720

cuaccgugga uauugagcuu gugacaacca ccgucuccaa cauggcugag gugcgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acacaagggg      840

aggccuaccu ggacaagcag ucugacacgc aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggucaca ugcgccaaau      960

uugcuugcuc caagaaaaug accggaaaga gcauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacggg ucccaacaca gcggcaugau cguuaaugac acagggcaug     1080

aaaccgauga gaaccgggcg aagguggaga uuacgcccaa uucucccaga gccgaagcua     1140

cacuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgccggcg ccgacacagg gaccccgcau uggaacaaua     1320

aagaggcucu gguggaauuu aaggaugccc acgcgaaacg gcagacaguc gugguucucg     1380

gaucucagga gggcgcugug cauacggccc uggccggggc ucuugaggcc gaaauggacg     1440

gcgcuaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua ccgccgcguu cacauuuacc aagauuccgg     1560

ccgagacacu gcauggaacc gugacgguug aagugcagua cgcuggcacc gacgggccuu     1620

gcaaaguccc cgcccaaaug gcuguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacagccaa ccccguuauu accgagucca cggaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggcguuuga agccacagug agaggggcua     1860

aacggauggc cguccuggga gauaccgcuu gggacuucgg cuccguuggg ggagcccuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacccuccu gauguggcug ggacucaaca     2040

caaagaaugg cagcaucucc cugauguguc uugcgcuugg gggagugcuc auuuuccuga     2100

gcacggccgu gucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6813
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 2 opt

<400>  6813
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg cgaagccauu agcuucccca      180

caacccuggg gaugaacaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gagccgcaga gccguuaccc uucccuccca uucuacacgg aaacugcaaa      420

cccgcagcca gacauggcuc gaguccagag aguacaccaa gcaccugauc cggguggaaa      480

acuggauuuu ucgcaauccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcagcucuac gucccagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acagcauuag augcaucggg guuucuaacc gggacuuugu ggagggaaug uccggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucagcaa uauggccgag gugcgcucuu      780

acugcuauga agccuccauc agcgacaugg cuuccgauuc uagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag agcgacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgccaaau      960

uugccugcag caagaaaaug acagggaagu cuauucagcc ugaaaaucuc gaguacagaa     1020

ucaugcuguc cguccacgga agccaacacu cuggcaugau cguuaacgac accgggcaug     1080

aaacggauga gaaucgggcu aagguggaga uuacaccgaa cagccccaga gcggaagcca     1140

cccuuggagg cuucggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

cugaccuuua uuaccugaca augaauaaca aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaauaaca     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaagccagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaaacuuc     1500

ggcugaaagg ggucucuuau ucccucugua cagcugccuu caccuuuagc aagauuccgg     1560

cggagacacu gcauggaacc gugacggaag agguucagua cgccggcacc gacgggccuu     1620

gcaaagugcc cgcucaaaug gccgucgaca ugcagacacu gaccccugug ggaagacuca     1680

ucacagcuaa ucccgugauu accgaaucua cggagaacuc caagaugaug cuggagcuug     1740

aucccccuuu cggcgacagc uacaucguua ucggggucgg cgaaaagaag auuacacacc     1800

acuggcaucg cuccggaucu accaucggca aggccuuuga ggcgacagug agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cagcgucggg ggagcucuca     1920

acucccuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaucu cuguucggag     1980

gcaugagcug guuuucccaa auucuuaucg ggacccuccu gauguggcug ggacucaaua     2040

caaagaacgg cagcaucucu cugauguguc uugcucucgg gggagugcug auuuuccugu     2100

ccacggcggu uagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6814
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 2 opt

<400>  6814
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg cgaagccauu agcuucccca      180

caacccuggg gaugaacaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gagccgcaga gccguuaccc uucccuccca uucuacacgg aaacugcaaa      420

cccgcagcca gacauggcuc gaguccagag aguacaccaa gcaccugauc cggguggaaa      480

acuggauuuu ucgcaauccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcagcucuac gucccagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acagcauuag augcaucggg guuucuaacc gggacuuugu ggagggaaug uccggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucagcaa uauggccgag gugcgcucuu      780

acugcuauga agccuccauc agcgacaugg cuuccgauuc uagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag agcgacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgccaaau      960

uugccugcag caagaaaaug acagggaagu cuauucagcc ugaaaaucuc gaguacagaa     1020

ucaugcuguc cguccacgga agccaacacu cuggcaugau cguuaacgac accgggcaug     1080

aaacggauga gaaucgggcu aagguggaga uuacaccgaa cagccccaga gcggaagcca     1140

cccuuggagg cuucggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

cugaccuuua uuaccugaca augaauaaca aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacaccggcg cugacacagg gacgccccau uggaauaaca     1320

aagaggcccu gguggaauuu aaggaugccc acgcuaaacg gcagaccguc gugguucucg     1380

gaagccagga gggcgccgug cauacagcgc uggcuggggc ccuugaggcc gaaauggacg     1440

gcgcuaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaaacuuc     1500

ggcugaaggg ggucucuuau ucccucugua ccgccgcuuu cacauuuacc aagauuccgg     1560

ccgagacgcu gcauggaacc gugacaguug aagugcagua cgcgggcacc gacgggccuu     1620

gcaaaguccc cgcccaaaug gcuguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacggccaa ucccguuauu acagagggca ccgaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggggacucu uacaucguca ucggcguggg agagaaaaag auuacacacc     1800

acuggcaucg cuccggcagc accaucggga aggcuuuuga agccaccgug cggggagcga     1860

aaagaauggc cguccugggc gauacagcuu gggacuucgg guccguggga ggcguucuca     1920

acucucuggg gaagggcauu caccagaucu ucggagccgc uuuuaaaagc cuguucggcg     1980

ggauguccug guuuucucaa auucuuaucg gaacgcuccu gauguggcug ggccucaaua     2040

ccaagaacgg gagcaucucc cugauguguc uugcccuggg aggcgugcuc auuuuccuga     2100

gcacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6815
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 2 opt

<400>  6815
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg cgaagccauu agcuucccca      180

caacccuggg gaugaacaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gagccgcaga gccguuaccc uucccuccca uucuacacgg aaacugcaaa      420

cccgcagcca gacauggcuc gaguccagag aguacaccaa gcaccugauc cggguggaaa      480

acuggauuuu ucgcaauccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcagcucuac gucccagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acagcauuag augcaucggg guuucuaacc gggacuuugu ggagggaaug uccggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucagcaa uauggccgag gugcgcucuu      780

acugcuauga agccuccauc agcgacaugg cuuccgauuc uagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag agcgacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgccaaau      960

uugccugcag caagaaaaug acagggaagu cuauucagcc ugaaaaucuc gaguacagaa     1020

ucaugcuguc cgugcacgga agccaacacu cuggcaugau cguuaacgac accgggcaug     1080

aaacggauga gaaucgggcu aagguggaga uuacaccgaa cagccccaga gcggaagcca     1140

cccuuggagg cuucggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

cugaccuuua uuaccugaca augaauaaca aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaauaaca     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaagccagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucucuuau ucccucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ucccguuauu acggagagca cagaaaacuc uaagaugaug cuggagcuug     1740

aucccccuuu cggcgacucc uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cagcggaucc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cucugucggg ggagcucuca     1920

acagccuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaucc cuguucggag     1980

gcaugucuug guuuagccaa auucuuaucg ggacacuccu gauguggcug ggacucaaua     2040

cgaagaacgg cuccaucagc cugauguguc uugcucuugg gggaguucuc auuuuccugu     2100

cuaccgcggu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6816
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 2 opt

<400>  6816
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc uguugaggug accagacggg      120

gaaacgccua cuauauguac cuugaccgcu ccgaugcggg cgaagcuauu ucuuucccca      180

caaccauggg gaugaauaag ugcuauaucc agauuaugga ccugggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcucg acgaaggcgu ggagccggau gacgucgauu      300

guuggugcaa caccacaucc accugggugg uuuaugggac gugucaucac aaaaagggag      360

aagccagacg gucucgcaga gcugugaccc ugcccagcca uuccacacgg aaacuucaaa      420

cccgcucuca gacauggcug gagagcagag aguacaccaa gcaccucauc cgggucgaaa      480

auuggauuuu ucgcaacccu ggcuucgccc uggcugccgc ggccaucgcu uggcugcucg      540

gcuccagcac gucucagaaa gugaucuauc ugguuaugau ucuucugauc gcccccgcuu      600

acuccauuag augcaucggg gugagcaauc gggacuuugu cgagggaaug ucuggcggga      660

caugggugga ugugguccuc gaacauggag gcuguguuac cgugauggcc caggacaagc      720

cuacagugga uauugagcug gucaccacca caguguccaa cauggcggag guucgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aggccuaccu ugacaagcag ucugacaccc aguaugugug caaacggaca cuggucgauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgcuaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccug gaguacagaa     1020

ucaugcucuc uguucacgga ucccaacaca gcggcaugau cgugaaugac accgggcaug     1080

aaacggauga gaaccgggcc aaggucgaga uuacaccgaa uagccccaga gcugaagcga     1140

cccugggagg cuucggcagc cuugggcugg acugugagcc ucgcaccgga cucgauuuuu     1200

ccgaccugua uuaccuuaca augaacaaua aacacuggcu ggugcauaag gaaugguucc     1260

acgauauccc ccucccuugg cacgccggcg cugacaccgg gacaccccau uggaacaaua     1320

aagaggcccu ggucgaauuu aaggaugccc acgcuaaacg gcagacggug guugugcugg     1380

gaucucagga gggcgccguc cauaccgcgc ucgcuggggc ccuggaggcc gaaauggacg     1440

gcgcuaaggg aagacuuagc uccggccacc ugaaaugccg ccucaagaug gauaagcugc     1500

ggcuuaaagg ggugagcuau ucucugugua cagccgcuuu caccuuuaca aagauuccgg     1560

ccgagacccu ccauggaacg guuaccgugg aaguccagua cgcgggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcuguggaca ugcagacccu gacaccuguu ggaagacuga     1680

ucaccgccaa ccccgucauu acggagucca cagaaaacag caagaugaug cucgagcugg     1740

aucccccuuu cggcgacucu uacaucguga ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggcuuuuga agccaccguc agaggggcga     1860

aacggauggc cgugcuugga gauaccgcuu gggacuucgg cuccguuggg ggagcccuca     1920

auucucucgg caaggggauu caccagaucu ucggcgcugc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucugaucg ggacacuucu cguguggcug ggacugaaca     2040

cgaagaaugg cagcaucucc cucauguguc uggcccuugg gggaguccug auuuuccuca     2100

gcaccgcugu gucugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6817
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 2 opt

<400>  6817
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg cgaagccauu agcuucccca      180

caacccuggg gaugaacaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gagccgcaga gccguuaccc uucccuccca uucuacacgg aaacugcaaa      420

cccgcagcca gacauggcuc gaguccagag aguacaccaa gcaccugauc cggguggaaa      480

acuggauuuu ucgcaauccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcagcucuac gucccagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acagcauuag augcaucggg guuucuaacc gggacuuugu ggagggaaug uccggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucagcaa uauggccgag gugcgcucuu      780

acugcuauga agccuccauc agcgacaugg cuuccgauuc uagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag agcgacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgccaaau      960

uugccugcag caagaaaaug acagggaagu cuauucagcc ugaaaaucuc gaguacagaa     1020

ucaugcuguc cgugcacgga agccaacacu cuggcaugau cguuaacgac accgggcaug     1080

aaacggauga gaaucgggcu aagguggaga uuacaccgaa cagccccaga gcggaagcca     1140

cccuuggagg cuucggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

cugaccuuua uuaccugaca augaauaaca aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaauaaca     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaagccagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucucuuau ucccucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ucccguuauu acggagagca cagaaaacuc uaagaugaug cuggagcuug     1740

aucccccuuu cggcgacucc uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cagcggaucc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cucugucggg ggagcucuca     1920

acagccuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaucc cuguucggag     1980

gcaugucuug guuuagccaa auucuuaucg ggacacuccu gauguggcug ggacucaaua     2040

cgaagaacgg cuccaucagc cugguuuguc uugcucuugg gggagugcuc auuuuccugu     2100

cuaccgcggu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6818
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 2 opt

<400>  6818
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg cgaagccauu agcuucccca      180

caacccuggg gaugaacaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gagccgcaga gccguuaccc uucccuccca uucuacacgg aaacugcaaa      420

cccgcagcca gacauggcuc gaguccagag aguacaccaa gcaccugauc cggguggaaa      480

acuggauuuu ucgcaauccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcagcucuac gucccagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acagcauuag augcaucggg guuaacaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugguuuguc uugcucuugg gggagugcuc auuuuccuga     2100

gcaccgcggu cucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6819
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 2 opt

<400>  6819
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc uguugaggug accagacggg      120

gauccgccua cuauauguac cuugaccgcu cugaugcggg cgaagcuauu agcuucccca      180

caacccuggg gaugaacaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacgucgauu      300

guuggugcaa uaccacaucu accugggugg uuuaugggac gugucaucac aaaaagggag      360

aagccagacg gagccgcaga gcugugaccc uucccuccca uucuacacgg aaacugcaaa      420

cccgcagcca gacauggcuc gaguccagag aguacaccaa gcaccugauc cgggucgaaa      480

acuggauuuu ucgcaauccu ggcuucgccc uggcugccgc ggccaucgcu uggcuccugg      540

gcagcucuac gucccagaaa gugaucuauc uuguuaugau ucugcucauc gcccccgcuu      600

acagcauuag augcaucggg gugucuaacc gggacuuugu cgagggaaug uccggcggga      660

caugggugga ugugguccug gaacauggag gcuguguuac cgugauggcc caggacaagc      720

cugcggugga uauugagcuu gucacaacca ccgugagcaa uauggccgag guucgcucuu      780

acugcuauga agccuccauc agcgacaugg cuuccgauuc uagauguccc acacaagggg      840

aggcuuaccu ggacaagcag agcgacacgc aguaugugug caaacggacc cucgucgauc      900

gcggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugaca ugcgccaaau      960

uugccugcag caagaaaaug accgggaagu cuauucagcc ugaaaaucuc gaguacagaa     1020

ucaugcuguc cguucacgga agccaacacu cuggcaugau cgugaacgac acagggcaug     1080

aaaccgauga gaaucgggcu aaggucgaga uuacgccgaa cagccccaga gcggaagcca     1140

cacuuggagg cuucggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

cugaccuuua uuaccugacc augaauaaca aacacuggcu cgugcauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacacagg gacaccccau uggaauaaca     1320

aagaggcccu ggucgaauuu aaggaugcuc acgccaaacg gcagaccgug guugugcucg     1380

gaagccagga gggcgcgguc cauacggcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggugucuuau ucccucugua ccgcugccuu cacauuuacc aagauuccgg     1560

cggagacacu gcauggaacc guuacggugg aaguccagua cgccggcacc gacgggccuu     1620

gcaaagugcc cgcucaaaug gccguggaca ugcagacacu gaccccuguu ggaagacuca     1680

ucacagcuaa ucccgucauu accgagagca cggaaaacuc uaagaugaug cuggagcuug     1740

aucccccuuu cggcgacucc uacaucguga ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cagcggaucc accaucggca aggccuuuga agcgacaguc agaggggcca     1860

aacggauggc ugugcuggga gauaccgccu gggacuucgg cucuguuggg ggagcucuca     1920

acagccuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaucc cuguucggag     1980

gcaugucuug guuuagccaa auucuuaucg ggacccuccu gguguggcug ggacucaaua     2040

caaagaacgg cuccaucagc cugacguguc uugcucuugg gggaguccuc auuuuccugu     2100

cuaccgcggu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6820
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 2 opt

<400>  6820
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc uguugaggug accagacggg      120

gauccgccua cuauauguac cuugaccgcu cugaugcggg cgaagcuauu agcuucccca      180

caacccuggg gaugaacaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacgucgauu      300

guuggugcaa uaccacaucu accugggugg uuuaugggac gugucaucac aaaaagggag      360

aagccagacg gagccgcaga gcugugaccc uucccuccca uucuacacgg aaacugcaaa      420

cccgcagcca gacauggcuc gaguccagag aguacaccaa gcaccugauc cgggucgaaa      480

acuggauuuu ucgcaauccu ggcuucgccc uggcugccgc ggugaucgcc uggcuccugg      540

gcagcucuac gucccagaaa guuaucuauc uugugaugau ucugcucauc gcucccgccu      600

acagcauuag augcaucggg gucucuaacc gggacuuugu ggagggaaug uccggcggga      660

caugggugga ugucguucug gaacauggag gcugugugac cgugauggcu caggacaagc      720

cuacagucga uauugagcuu gugaccacca caguuagcaa uauggccgag gugcgcucuu      780

acugcuauga agcguccauc agcgacaugg ccuccgauuc uagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag agcgacaccc aguaugucug caaacggaca cucguggauc      900

gcggaugggg caacggcugu gggcuguucg gaaagggcuc ccugguuacc ugcgccaaau      960

uugcuugcag caagaaaaug acagggaagu cuauucagcc ugaaaaucuc gaguacagaa     1020

ucaugcuguc cgugcacgga agccaacacu cuggcaugau cgucaacgac accgggcaug     1080

aaacggauga gaaucgggcc aagguggaga uuacaccgaa cagccccaga gccgaagcua     1140

cccuuggagg cuucggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

cugaccuuua uuaccugaca augaauaaca aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcgggcg ccgacaccgg gacaccccau uggaauaaca     1320

aagaggcucu gguggaauuu aaggaugccc acgccaaacg gcagacgguu gugguccucg     1380

gaagccagga gggcgcugug cauaccgccc uggcgggggc ucuugaggcc gaaauggacg     1440

gcgccaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg gguuucuuau ucccucugua cagcugccuu caccuuuaca aagauuccgg     1560

cugagacccu gcauggaacg gugaccgucg aagugcagua cgccggcaca gacgggccuu     1620

gcaaagugcc cgcgcaaaug gccguugaca ugcagacccu gacaccuguc ggaagacuca     1680

ucaccgcuaa ucccgugauu acggagagca cagaaaacuc uaagaugaug cuggagcuug     1740

aucccccuuu cggcgacucc uacaucguga ucggggucgg cgagaaaaag auuacccacc     1800

acuggcaucg cagcggaucc acaaucggca aggccuuuga agcuaccgug agaggggcca     1860

aacggauggc gguucuggga gauaccgccu gggacuucgg cucugucggg ggagcucuca     1920

acagccuggg caaggggauu caccagaucu ucggcgccgc uuuuaaaucc cuguucggag     1980

gcaugucuug guuuagccaa auucuuaucg ggacacuccu gguguggcug ggacucaaua     2040

cgaagaacgg cuccaucagc cugaccuguc uugcccuugg gggagugcuc auuuuccugu     2100

cuacagccgu uuccgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6821
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 2 opt

<400>  6821
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc uguugaggug accagacggg      120

gauccgccua cuauauguac cuugaccgcu cugaugcggg cgaagcuauu agcuucccca      180

caacccuggg ggugaacaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgagugc ccuaugcugg acgaaggcgu cgagccggau gacguggauu      300

guuggugcaa uaccacaucu accuggguug uguaugggac gugucaucac aaaaagggag      360

aagccagacg gagccgcaga gcugucaccc uucccuccca uucuacacgg aaacugcaaa      420

cccgcagcca gacauggcuc gaguccagag aguacaccaa gcaccugauc cggguggaaa      480

acuggauuuu ucgcaauccu ggcuucgccc uggcugccgc ggccaucgcu uggcuccugg      540

gcagcucuac gucccagaaa guuaucuauc uugugaugau ucugcucauc gcccccgcuu      600

acagcauuag augcaucggg gucucuaacc gggacuuugu ggagggaaug uccggcggga      660

caugggugga ugucguucug gaacauggag gcugugugac cgugauggcc caggacaagc      720

cuacagucga uauugagcuu gugaccacca caguuagcaa uauggcggag gugcgcucuu      780

acugcuauga agccuccauc agcgacaugg cuuccgauuc uagauguccc acgcaagggg      840

aggccuaccu ggacaagcag agcgacaccc aguaugucug caaacggaca cucguggauc      900

gcggaugggg caacggcugu gggcuguucg gaaagggcuc ccugguuacc ugcgcuaaau      960

uugccugcag caagaaaaug acagggaagu cuauucagcc ugaaaaucuc gaguacagaa     1020

ucaugcuguc cgugcacgga agccaacacu cuggcaugau cgucaacgac accgggcaug     1080

aaacggauga gaaucgggcc aagguggaga uuacaccgaa cagccccaga gcugaagcga     1140

cccuuggagg cuucggcucc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

cugaccuuua uuaccugaca augaauaaca aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgccggcg cugacaccgg gacaccccau uggaauaaca     1320

aagaggcccu gguggaauuu aaggaugccc acgcuaaacg gcagacgguu gugguccucg     1380

gaagccagga gggcgccgug cauaccgcgc uggcuggggc ccuugaggcc gaaauggacg     1440

gcgcuaaggg aagacugucc agcggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg gguuucuuau ucccucugua cagccgcuuu caccuuuaca aagauuccgg     1560

ccgagacccu gcauggaacg gugaccgucg aagugcagua cgcgggcaca gacgggccuu     1620

gcaaagugcc cgcccaaaug gcuguugaca ugcagacccu gacaccuguc ggaagacuca     1680

ucaccgccaa ucccgugauu acggagagca cagaaaacuc uaagaugaug cuggagcuug     1740

aucccccuuu cggcgacucc uacaucguga ucggggucgg cgauaaaaag auuacccacc     1800

acugguaucg cagcggaucc acaaucggca aggcuuuuga ggccaccgug agaggggcga     1860

aacggauggc cguucuggga gacaccgcuu gggauuucgg cucugucggg ggagcccuca     1920

acagccuggg caaggggauu caucagaucu ucggcgcugc cuuuaaaucc cuguucggag     1980

gcaugucuug guuuagccaa auucuuaucg ggacacuccu gguguggcug ggacucaaua     2040

cgaagaacgg cuccaucagc cugaccuguc uugcccuugg gggagugcuc auuuuccugu     2100

cuacagcugu uuccgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6822
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 2 opt

<400>  6822
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc uguugaggug accagacggg      120

gauccgccua cuauauguac cuugaccgcu cugaugcggg caaggcuauu agcuucccca      180

caacccuggg ggugaacaaa ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaauguc cuacgaaugc ccuaugcugg acgagggcgu cgaaccggau gacguggauu      300

guuggugcaa uaccacaucu accuggguug uguaugggac gugucaucac aagaaaggag      360

aggccagacg gagccgcaga gcugucaccc uucccuccca uucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguacaccaa acaccugauc cggguggaga      480

acuggauuuu ucgcaauccu ggcuucgccc uggcugccgc ggccaucgcu uggcuccugg      540

gcagcucuac gucccagaag guuaucuauc uugugaugau ucugcucauc gcccccgcuu      600

acagcauuag augcaucggg gucucuaacc gggacuuugu ggaaggaaug uccggcggga      660

caugggugga ugucguucug gagcauggag gcugugugac cgugauggcc caggacaagc      720

cuacagucga uauugaacuu gugaccacca caguuagcaa uauggcggag gugcgcucuu      780

acugcuauga ggccuccauc agcgacaugg cuuccgauuc uagauguccc acgcaagggg      840

aagccuaccu ggacaaacag agcgacaccc aguaugucug caagcggaca cucguggauc      900

gcggaugggg caacggcugu gggcuguucg gaaaaggcuc ccugguuacc ugcgcuaagu      960

uugccugcag caaaaagaug acagggaagu cuauucagcc ugagaaucuc gaauacagaa     1020

ucaugcuguc cgugcacgga agccaacacu cuggcaugau cgucaacgac auugggcaug     1080

agaccgauga aaaucgggcc aaaguggaga ucacgccgaa cagccccaga gcugaggcga     1140

cacuuggagg cuucggcucc cuggggcucg acugugaacc ucgcaccgga cuggauuuuu     1200

cugaccuuua uuaccugacc augaauaaca agcacuggcu cguccauaaa gagugguucc     1260

acgauauucc ccugccuugg cacgccggcg cugacacagg gacaccccau uggaauaaca     1320

aggaagcccu gguggaguuu aaagaugccc acgcuaagcg gcagacaguu gugguccucg     1380

gaagccagga aggcgccgug cauacggcgc uggcuggggc ccuugaggcc gagauggacg     1440

gcgcuaaagg aagacugucc agcggccacc ucaagugccg ccugaagaug gauaaacuuc     1500

ggcugaaggg gguuucuuau ucccucugua ccgccgcuuu cacauuuacc aaaaucccgg     1560

ccgaaacacu gcauggaacc gugacggucg aggugcagua cgcgggcacc gacgggccuu     1620

gcaaggugcc cgcccaaaug gcuguugaca ugcagacacu gaccccuguc ggaagacuca     1680

uuacagccaa ucccgugauc accgaaagca cggagaacuc uaaaaugaug cuggaacuug     1740

aucccccuuu cggcgacucc uacaucguga uuggggucgg cgauaagaag aucacacacc     1800

acuggcaucg cagcggaucc accauuggca aggcuuuuga ggccacagug agaggggcga     1860

aacggauggc cguucuggga gacaccgcuu gggauuucgg cucugucggg ggagcccuca     1920

acagccuggg caaagggauc caccagauuu ucggcgcugc cuuuaagucc cuguucggag     1980

gcaugucuug guuuagccaa auccuuaucg ggacccuccu gguguggcug ggacucaaua     2040

ccaaaaacgg cuccauuagc cugacguguc uugcccucgg gggagugcug aucuuccugu     2100

cuaccgcugu uuccgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6823
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 2 opt

<400>  6823
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccugacaa cggccauggc ugccgagauc accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauu agcuucgcca      180

caacccuggg ggucaacaaa ugccacguuc agaucaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ggaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcuguuaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauu aagguggaga      480

acuggaucuu ucggaauccc ggcuucacgc uggucgccgu ggcgaucgcc uggcuccugg      540

gcagcucuac aucccagaag guuauuuacc uugugaugau ccugcucauu gcuccugccu      600

auagcauccg cugcauuggg gucucuaaca gagacuuugu ggaaggaaug uccggcggga      660

ccugggugga ugucguucug gagcacggag gcugugugac agugauggcu caggacaaac      720

ccaccgucga uaucgaacuu gugaccacaa cgguuagcaa uauggccgag gugcggucuu      780

acugcuacga ggcguccauu agcgacaugg ccuccgauuc ucgcuguccu acccaagggg      840

aggcuuaucu ggacaagcag agcgacacac aguacgucug caaaagaacc cucguggauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccugguuaca ugcgccaaau      960

uuaccugcag caagaagaug acggggaaau cuauccagcc cgaaaaucuc gaauaucgca     1020

ucaugcuguc cgugcacgga agccaacauu cuggcaugau ugucaacgac acagggcacg     1080

agaccgauga aaauagagcu aagguggagg ucaccccuaa cagcccgcgg gccgaggcca     1140

cacuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga cuggauuuuu     1200

cugaccuuua cuaucugaca augaauaaca aacauuggcu cgugcacaag gagugguucc     1260

acgauauccc ucugcccugg caugcuggcg cggacacggg gaccccucac uggaauaaca     1320

aagaagcccu gguugaguuu aaggaugcuc augccaaacg ccagacagug gucgugcucg     1380

gaagccagga aggcgccguu cacaccgcuc uggccggggc gcuugaggcu gagauggacg     1440

gcgccaaggg acggcuguuc uccggccauc ucaagugcag acugaaaaug gauaaacuuc     1500

gccugaaggg ggugagcuac ucucucugua cagccgcuuu uaccuucacg aagguccccg     1560

ccgaaacccu gcacggaaca gugaccgugg agguucagua ugcuggcaca gacgggccgu     1620

gcaaaguccc ugcccaaaug gcgguggaca ugcagacccu gacgcccgug ggacggcuca     1680

uuacagccaa uccugucauc accgaaucca cagagaacag caagaugaug cuggaacuug     1740

auccucccuu uggcgacucu uacauuguga ucgggguugg cgauaagaaa aucacccacc     1800

auuggcacag auccggaagc accauuggca aggcuuucga ggccacagug cgcggggcua     1860

aacggauggc cguccuggga gacacggcgu gggauuucgg cuccguuggg ggaguguuua     1920

acucucucgg caaggggauc caucagauuu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccugauug ggacccuucu cguguggcug ggacugaaua     2040

caaagaacgg cagcaucucc cucaccuguc uggcccuugg gggagucaug aucuuccuga     2100

gcacagcugu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6824
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 2 opt

<400>  6824
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caaaccuggg ggugaauaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuaccauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa cacaaccucu acaugggugg ucuacgggac cugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugacgc uuccguccca cucuacccgg aagcugcaaa      420

cacgcagcca gaccuggcuc gaauccagag aguauacaaa acaucugauc aagguugaga      480

auuggaucuu ucggaacccc ggcuucgccc uggcggccgu ggcuauugcc uggcuccugg      540

gcagcucuac cucccagaag gucaucuacc uugugaugau ucugcucauc gcuccugccu      600

auagcauucg cugcaucggg guuucuaaua gagacuuugu ggaaggaaug uccggcggga      660

cgugggucga uguggugcug gagcacggag gcugugucac aguuauggcg caggacaaac      720

ccaccgugga uauugaacuu gugacaacca ccgucagcaa cauggccgag gugcggucuu      780

acugcuacga ggcuuccauc agcgacaugg ccuccgauuc ucgcuguccu acacaagggg      840

aggcuuaucu ggacaagcag agcgacacgc aguacguuug caaaagaacc cucguggauc      900

ggggaugggg caauggcugu gggcuguucg gaaagggcuc ccuggucaca ugcgccaaau      960

uuaccugcag caagaagaug acagggaaau cuauccagcc cgaaaaccuc gaauaucgca     1020

uuaugcuguc cgugcacgga agccaacauu cuggcaugau cguuaaugac auugggcacg     1080

agaccgauga aaacagagcc aagguggagg ucacgccuaa uagcccgcgg gcugaggcga     1140

cacuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga cuggauuuuu     1200

cugaccuuua cuaucugacc augaacaaua aacauuggcu cgugcacaag gagugguucc     1260

acgauauccc ucugcccugg caugccggcg cugacacagg gaccccucac uggaacaaua     1320

aagaagcccu ggucgaguuu aaggaugccc augcuaaacg ccagacagug guugugcucg     1380

gaagccagga aggcgccguc cacacggcgc uggcuggggc ccuugaggcc gagauggacg     1440

gcgcuaaggg acggcuguuc uccggccauc ucaagugcag acugaaaaug gauaaacuuc     1500

gccugaaggg ggugagcuac ucucucugua ccgccgcuuu uacauucacc aagguucccg     1560

ccgaaacacu gcacggaacc gugacggucg aggugcagua ugcgggcacc gacgggccgu     1620

gcaaagugcc ugcccaaaug gcuguugaca ugcagacacu gacccccguc ggacggcuca     1680

uuacagccaa cccugugauc accgaaucca cggagaacag caagaugaug cuggaacuug     1740

auccucccuu uggcgacucu uacaucguga uuggggucgg cgauaagaaa aucacacacc     1800

auuggcacag auccggaagc accauuggca aggcuuucga ggccacagug cgcggggcga     1860

aacggauggc cguucuggga gacaccgcuu gggauuucgg cuccgucggg ggaguguuua     1920

auucucucgg caagggggug caucagaucu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucugaucg ggacccuucu cguuuggcug ggacugaaca     2040

caaagaaugg cagcaucucc cucacguguc uggcccuugg gggagugaug auuuuccuga     2100

gcaccgccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6825
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 2 opt

<400>  6825
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauc aagguugaga      480

acuggaucuu ucggaauccc ggcuucgccc ugguggcggu cgccauugcu uggcuccugg      540

gcagcucuac gucccagaag gugaucuacc uuguuaugau ucugcucauc gccccugcuu      600

auagcauucg cugcaucggg gugucuaaca gagacuuugu cgaaggaaug uccggcggga      660

caugggugga ugugguccug gagcacggag gcuguguuac cgugauggcc caggacaaac      720

ccacagugga uauugaacuu gucaccacca cagugagcaa uauggcggag guucggucuu      780

acugcuacga ggccuccauc agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgugug caaaagaaca cucgucgauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccuggugacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

uuaugcuguc cguucacgga agccaacauu cuggcaugau cgugaacgac auugggcacg     1080

agacggauga aaauagagcc aaggucgagg ugacaccuaa cagcccgcgg gccgaggcua     1140

cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga cuggauuuuu     1200

cugaccuuua cuaucugaca augaauaaca aacauuggcu cguccacaag gagugguucc     1260

acgauauccc ucugcccugg caugcgggcg ccgacaccgg gacaccucac uggaauaaca     1320

aagaagcucu gguggaguuu aaggaugccc augccaaacg ccagacgguu gugguccucg     1380

gaagccagga aggcgcugug cacaccgccc uggcgggggc ucuugaggcc gagauggacg     1440

gcgccaaggg acggcuguuc uccggccauc ucaagugcag acugaaaaug gauaaacuuc     1500

gccugaaggg gguuagcuac ucucucugua cagcugccuu uaccuucaca aaggugcccg     1560

cugaaacccu gcacggaacg gucaccgugg aggugcagua ugccggcaca gacgggccgu     1620

gcaaaguucc ugcgcaaaug gccgucgaca ugcagacccu gacacccgug ggacggcuca     1680

uuaccgcuaa uccugugauc acggaaucca cagagaacag caagaugaug cuggaacuug     1740

auccucccuu uggcgacucu uacaucguca uugggguggg cgauaagaaa aucacccacc     1800

auuggcacag auccggaagc acaauuggca aggccuucga ggcuaccguu cgcggggcca     1860

aacggauggc ggugcuggga gacaccgccu gggauuucgg cuccgucggg ggaguguuua     1920

acucucucgg caaggggauc caucagauuu ucggcgcugc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccugaucg ggacacuucu cguuuggcug ggacugaaua     2040

cgaagaacgg cagcauuucc cucaccuguc uggcucuugg gggagugaug aucuuccuga     2100

gcacagccgu cucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6826
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 2 opt

<400>  6826
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caaaccuggg ggugaauaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuaccauguc cuaugaaugc cccaugcugg acgagggcgu ugaacccgau gacguggauu      300

guuggugcaa cacaaccucu acaugggugg ucuacgggac cugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcugugacgc uuccguccca cucuacccgg aagcugcaaa      420

cacgcagcca gaccuggcuc gaauccagag aguauacaaa acaucugauc aagguugaga      480

auuggaucuu ucggaacccc ggcuucgccc uggcggccgu ggcuauugcc uggcuccugg      540

gcagcucuac cucccagaag gucaucuacc uugugaugau ucugcucauc gcuccugccu      600

auagcauucg cugcaucggg guuucuaaua gagacuuugu ggaaggaaug uccggcggga      660

cgugggucga uguggugcug gagcacggag gcugugucac aguuauggcg caggacaaac      720

ccaccgugga uauugaacuu gugacaacca ccgucagcaa cauggccgag gugcggucuu      780

acugcuacga ggcuuccauc agcgacaugg ccuccgauuc ucgcuguccu acacaagggg      840

aggcuuaucu ggacaagcag agcgacacgc aguacguuug caaaagaacc cucguggauc      900

ggggaugggg caauggcugu gggcuguucg gaaagggcuc ccuggucaca ugcgccaaau      960

uuaccugcag caagaagaug acagggaaau cuauccagcc cgaaaaccuc gaauaucgca     1020

uuaugcuguc cgugcacgga agccaacauu cuggcaugau cguuaaugac gagaacagag     1080

ccaaggugga agucaccccu aauagcccgc gggcugaggc gacgcuuggg ggauucggcu     1140

cccugggccu cgauugugag cccagaacag ggcuggacuu uucugaucuu uacuaucuga     1200

ccaugaacaa uaaacacugg cucgugcaua aggaaugguu ccacgacauu ccucugcccu     1260

ggcacgccgg agcugauacc ggcacaccuc auuggaacaa uaaagaggcc cuggucgaau     1320

uuaaggacgc ccacgcuaaa cgccagaccg ugguugugcu cgggagccag gagggagccg     1380

uccauacagc gcuggcuggc gcccuugaag ccgagaugga uggggcuaag ggccggcugu     1440

ucuccggaca ccucaagugc agacugaaaa uggacaaacu ucgccugaag ggcgugagcu     1500

acucucucug uacggccgcu uuuaccuuca caaagguucc cgccgagacc cugcauggga     1560

cagugaccgu cgaagugcag uaugcgggaa cggauggccc gugcaaagug ccugcccaaa     1620

uggcuguuga caugcagacc cugacacccg ucgggcggcu caucaccgcc aacccuguga     1680

uuacagaguc caccgaaaac agcaagauga ugcuggagcu ugacccuccc uuuggagauu     1740

cuuacaucgu gaucggcguc ggggacaaga aaauuacgca ccacuggcau agauccggca     1800

gcacaaucgg aaaggcuuuc gaagccaccg ugcgcggcgc gaaacggaug gccguucugg     1860

gggauacagc uugggacuuc ggauccgugg gcggggucuu uaauucucuc ggaaagggca     1920

uucaccagau cuucggggcc gcuuuuaaaa gccuguucgg cggaaugucc ugguuuucuc     1980

aaauucugau cggcacccuu cucguguggc uggggcugaa caccaagaau ggaagcaucu     2040

cccucacaug ucuggcccuu ggcgggguua ugauuuuccu gagcacggcc gugucugcuu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6827
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 2 opt

<400>  6827
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccugacaa cggccauggc ugccgagauc accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauu agcuucgcca      180

caacccuggg ggucaacaaa ugccacguuc agaucaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ggaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcuguuaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauu aagguggaga      480

acuggaucuu ucggaauccc ggcuucgccc uggucgcggu ggccaucgcu uggcuccugg      540

gcagcucuac gucccagaag guuauuuacc uugugaugau ccugcucauu gccccugcuu      600

auagcauccg cugcauuggg gucucuaaca gagacuuugu ggaaggaaug uccggcggga      660

caugggugga ugucguucug gagcacggag gcugugugac cgugauggcc caggacaaac      720

ccacagucga uaucgaacuu gugaccacca caguuagcaa uauggcggag gugcggucuu      780

acugcuacga ggccuccauu agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgucug caaaagaaca cucguggauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccugguuacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

ucaugcuguc cgugcacgga agccaacauu cuggcaugau ugucaacgac acggggcacg     1080

agacagauga aaauagagcc aagguggagg ucaccccuaa cagcccgcgg gccgaggcua     1140

cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaacagga cuggauuuuu     1200

cugaccuuua cuaucugacc augaauaaca aacauuggcu cgugcacaag gagugguucc     1260

acgauauccc ucugcccugg caugcgggcg ccgacacagg gacgccucac uggaauaaca     1320

aagaagcucu gguugaguuu aaggaugccc augccaaacg ccagaccgug gucgugcucg     1380

gaagccagga aggcgcuguu cacacagccc uggcgggggc ucuugaggcc gagauggacg     1440

gcgccaaggg acggcuguuc uccggccauc ucaagugcag acugaaaaug gauaaacuuc     1500

gccugaaggg ggugagcuac ucucucugua ccgcugccuu uacauucacc aagguccccg     1560

cugaaacgcu gcacggaacc gugacagugg agguucagua ugccggcacc gacgggccgu     1620

gcaaaguccc ugcgcaaaug gccguggaca ugcagacacu gacccccgug ggacggcuca     1680

uuacggcuaa uccugucauc acagaaucca ccgagaacag caagaugaug cuggaacuug     1740

auccucccuu uggcgacucu uacauuguga ucgggguugg cgauaagaaa aucacacacc     1800

auuggcacag auccggaagc accauuggca aggccuucga ggcuaccgug cgcggggcca     1860

aacggauggc gguccuggga gacacagccu gggauuucgg cuccguuggg ggaguguuua     1920

acucucucgg caaggggauc caucagauuu ucggcgcugc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccugauug ggacgcuucu cguguggcug ggacugaaua     2040

ccaagaacgg cagcaucucc cucacauguc uggcucuugg gggagucaug aucuuccuga     2100

gcaccgccgu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6828
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 2 opt

<400>  6828
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccugacaa cggccauggc ugccgagauc accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauu agcuucgcca      180

caacccuggg ggucaacaaa ugccacguuc agaucaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ggaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguacgggac gugucaacac aagaaaggag      360

aggccagacg gagccgcaga gcuguuaccc uuccguccca uucuacacgg aagcugcaga      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaccugauu aagguggaga      480

acuggaucuu ucggaauccc ggcuucgccc uggucgcggu ggccaucgcu uggcuccugg      540

gcagcucuac gucccagaag guuauuuacc uugugaugau ccugcucauu gccccugcuu      600

auagcauccg cugcauuggg gucucuaaca gagacuuugu ggaaggaaug uccggcggga      660

caugggugga ugucguucug gagcauggag gcugugugac cgugauggcc caagacaaac      720

ccacagucga uaucgaacuu gugaccacca caguuagcaa uauggcggag gugcggucuu      780

acugcuacga ggccuccauu agcgacaugg cuuccgauuc ucgcuguccu acgcaggggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgucug caaaagaaca cucguggauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccugguuacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccaacc cgagaaucuc gaauaucgca     1020

ucaugcuguc cgugcacgga agccagcacu cuggcaugau ugucaacgac aucgggcaug     1080

agacggauga aaauagagcc aagguggagg ucacaccuaa cagcccgcgg gccgaggcua     1140

cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga cuggauuuuu     1200

cugaccuuua cuaucugaca augaauaaca aacacuggcu cgugcauaag gagugguucc     1260

acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau uggaauaaca     1320

aagaagcucu gguugaguuu aaggaugccc acgccaaacg ccagacggug gucgugcucg     1380

gaagccagga aggcgcuguu cauaccgccc uggcgggggc ucuugaggcc gagauggacg     1440

gcgccaaggg acggcuguuc uccggccacc ucaagugcag acugaaaaug gauaaacuuc     1500

gccugaaggg ggugagcuac ucucucugua cagcugucug caccgccgcu aaggugcccg     1560

ccgaaacacu gcauggaacc gugacgguug agguccaaua ugcgggcacc gacgggccgu     1620

guaaagugcc ugcccagaug gcuguggaca ugcagacacu gacccccguc ggacggcuca     1680

ucacagccaa uccugugauu accgaaucca cggagaacag caagaugaug cuggaacuug     1740

auccucccuu uggcgacucu uacaucguua ucgggguggg cgauaagaaa auuacacacc     1800

acuggcauag auccggaagc accaucggca aggcuuucga ggccacaguc cgcggggcga     1860

aacggauggc cgugcuggga gacaccgcuu gggauuuugg cuccguuggg ggaguguuca     1920

acucucucgg caaggggauu caccaaaucu ucggcgccgc uuuuaaaagc cuguucggag     1980

gcauguccug guuuucucag auucugaucg ggacccuucu cgucuggcug ggacugaaua     2040

caaagaacgg cagcaucucc cucacgugcc uggcccuugg gggagugaug auuuuccuga     2100

gcaccgccgu uucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6829
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 2 opt

<400>  6829
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uguggggcug cuccugacaa cggccauggc ugccgagauc accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauu agcuucgcca      180

caacccuggg ggucaacaaa ugccacguuc agaucaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ggaacccgau gacguggauu      300

guuggugcaa uaccacaucu accugggucg uguacgggac gugucaccau aagaaaggag      360

aggccagacg gagccgcaga gcuguuaccc uuccguccca cucuacacgg aagcugcaaa      420

cccgcagcca gacauggcuc gaauccagag aguauaccaa acaucugauu aagguggaga      480

acuggaucuu ucggaauccc ggcuucgccc uggucgcggu ggccaucgcu uggcuccugg      540

gcagcucuac gucccagaag guuauuuacc uugugaugau ccugcucauu gccccugcuu      600

auagcauccg cugcauuggg gucucuaaca gagacuuugu ggaaggaaug uccggcggga      660

caugggugga ugucguucug gagcacggag gcugugugac cgugauggcc caggacaaac      720

ccacagucga uaucgaacuu gugaccacca caguuagcaa uauggcggag gugcggucuu      780

acugcuacga ggccuccauu agcgacaugg cuuccgauuc ucgcuguccu acgcaagggg      840

aagccuaucu ggacaagcag agcgacaccc aguacgucug caaaagaaca cucguggauc      900

ggggaugggg caacggcugu gggcuguucg gaaagggcuc ccugguuacc ugcgcuaaau      960

uuacaugcag caagaagaug accgggaaau cuauccagcc cgagaaucuc gaauaucgca     1020

ucaugcuguc cgugcacgga agccaacauu cuggcaugau ugucaacgac gagaauagag     1080

ccaaggugga agucacgccu aacagcccgc gggccgaggc uacacuuggg ggauucggcu     1140

cccugggccu cgauugugag cccagaaccg ggcuggacuu uucugaucuu uacuaucuga     1200

ccaugaauaa caaacacugg cucgugcaua aggaaugguu ccacgacauc ccucugcccu     1260

ggcacgcggg agccgauaca ggcaccccuc auuggaauaa caaagaggcu cugguugaau     1320

uuaaggacgc ccacgccaaa cgccagacag uggucgugcu cgggagccag gagggagcug     1380

uucauacggc ccuggcgggc gcucuugaag ccgagaugga uggggccaag ggccggcugu     1440

ucuccggaca ccucaagugc agacugaaaa uggacaaacu ucgccugaag ggcgugagcu     1500

acucucucug uaccgcugcc uuuacauuca ccaagguccc cgcugagaca cugcauggga     1560

ccgugacggu ggaaguucag uaugccggaa ccgauggccc gugcaaaguc ccugcgcaaa     1620

uggccgugga caugcagaca cugacccccg uggggcggcu cauuacagcu aauccuguca     1680

ucaccgaguc cacggaaaac agcaagauga ugcuggagcu ugacccuccc uuuggagauu     1740

cuuacauugu gaucggcguu ggggacaaga aaaucacaca ccacuggcau agauccggca     1800

gcaccauugg aaaggccuuc gaagcuacag ugcgcggcgc caaacggaug gcgguccugg     1860

gggauaccgc cugggacuuc ggauccgugg gcgggguuuu uaacucucuc ggaaagggca     1920

uccaccagau uuucggggcu gccuuuaaaa gccuguucgg cggaaugucc ugguuuucuc     1980

aaauccugau uggcacccuu cucguguggc uggggcugaa uacaaagaac ggaagcaucu     2040

cccucacgug ucuggcucuu ggcgggguca ugaucuuccu gagcaccgcc gugucugccu     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  6830
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 2 opt

<400>  6830
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cuccugacaa cggccauggc ugccgagauu accagacggg      120

gauccgcgua cuauauguac cuugaccgcu cugaugcugg caaggccauc agcuucgcca      180

caacccuggg ggugaacaaa ugccacgucc agauuaugga ccucggacau augugugaug      240

cuacaauguc cuaugaaugc cccaugcugg acgagggcgu ugaaccugau gacguggauu      300

guuggugcaa uaccacaucu accugggugg ucuacgggac gugucaccau aagaaaggag      360

agaccagacg gagccgcaga uccgugucuc uucgguauca cuacacacgc aagcugcaaa      420

ccagaagcca gacauggcuc gaaucccggg aguauaaaaa gcaucugauc augguugaga      480

acuggaucuu ucgcaauccg ggcuucgcca uugugagcgu cgcuaucacc uggcugaugg      540

gcucucucac gucccagaag gugauuuacc ugguuaugau cgugcuuauu guccccgccu      600

acagcaucuc uugcauuggg guguccaaca gagaccuggu ggaaggaaug agcggcggga      660

caugggucga uguugugcuc gagcacggag gcugugugac cgaaauggcg caggacaaac      720

cuacagucga uaucgagcug gugaccauga ccguuucuaa uauggccgag gugcgguccu      780

auugcuacga agcuagccuu uccgacaugg ccucugcuag ccgcuguccc acacaagggg      840

agccuucccu ggauaagcag agcgacacgc agucugucug caaaagaacc cucggagacc      900

ggggcugggg caacgggugu ggaaucuuug gcaagggguc ccuggugaca ugcagcaaau      960

uuaccugcug uaagaagaug cccggaaaau cuauucagcc ugaaaaucug gaguaucgca     1020

ucaugcuccc gguucacggc agccaacauu ccgggaugau ugugaacgau aucggacacg     1080

aaacagacga gaauagagcc aaggucgagg ugacccccaa cucuccucgg gcggaagcca     1140

cgcugggcgg cuuugggagc cuuggacugg auugcgagcc cagaacaggc cucgacuucu     1200

ccgaucugua cuaucuuacc augaauaaca aacauuggcu gguccacaag gaaugguuuc     1260

acgacauucc ucuccccugg caugcugggg ccgauaccgg aacaccgcac uggaauaaca     1320

aagaggcucu gguggaguuc aaggacgccc augccaaacg ccagaccguu gugguccugg     1380

gcagccagga aggggcugug cacacagcgc ucgccggcgc ucuggaggcc gaaauggaug     1440

gagccaaggg ccggcuuuuc ucugggcauc ugaaguguag acucaaaaug gacaagcugc     1500

gccuuaaagg aguuuccuac agccugugca cggcugccuu caccuuuaca aaggugccug     1560

cggagacccu ccacggcaca gucaccgugg aagugcaguc ugcugggacg gauggacccu     1620

guaaaguucc ugcccaaaug gccgucgaca ugcagacccu gacacccgug ggccggcuga     1680

ucaccgcuaa ucccgugauc acagagucca ccgaaaacag caagaugaug cucgagcugg     1740

acccuccguu cggggauucc uauauuguca ucggcguggg agacaagaaa auuacgcacc     1800

auuggcacag aucuggcagc acaaucggga aggccuuuga ggcuaccguu cgcggagcca     1860

aacggauggc ggugcuuggc gauacagccu gggacuucgg guccgucgga ggcguguuca     1920

acucucuggg gaagggcauu caucagaucu uuggagcugc cuucaaaagc cucuuuggcg     1980

ggauguccug guucagccaa auccugauug gaacccugcu uguuuggcuc ggccugaaua     2040

ccaagaacgg gucuaucucc cugacaugcc ucgcucuggg aggcgugaug auuuuucuua     2100

gcacggccgu cucugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6831
<211>  2446
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 2 opt

<400>  6831
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcg       60

ugggcaucgu cgggcugcuc cugaccacag ccauggcugc cgagguuacg agacggggau      120

ccgcguacua uauguaccuu gaccgcaacg augcuggcga agccauuucu uuccccacca      180

cacuggggau gaauaagugc uauauccaga uuauggaccu cggacacaug ugugaugcca      240

ccaugagcua cgagugcccu augcuggacg aaggcgugga gccggaugac guggauuguu      300

ggugcaacac aaccuccaca ugggucgugu augggaccug ucaucacaaa aagggagaag      360

cuagacgguc ucgcagagcc guuacgcuuc ccagccauuc cacccggaaa cugcaaacac      420

gcucucagac cuggcucgag agcagagagu acacaaagca ccugauccgg guggaaaauu      480

ggauuuuucg caacccuggc uucgcucugg ccgcggccgc uaucgccugg cuccugggcu      540

ccagcaccuc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcu cccgccuacu      600

ccauuagaug caucgggguu agcaaucggg acuuugugga gggaaugucu ggcgggacgu      660

gggucgaugu ggugcuggaa cauggaggcu gugucacagu uauggcgcag gacaagccua      720

ccguggauau ugagcuugug acaaccaccg ucuccaacau ggccgaggug cgcagcuacu      780

gcuaugaagc cucuaucucc gacauggcua gcgauuccag augucccaca caaggggagg      840

cuuaccugga caagcagucu gacacgcagu auguuugcaa acggacccuc guggaucgcg      900

gauggggcaa uggcuguggg cuguucggaa agggcagccu ggucacaugc gccaaauuug      960

ccugcuccaa gaaaaugacc ggaaagagca uucagccuga aaaccucgag uacagaauca     1020

ugcugucugu gcacgggucc caacacagcg gcaugaucgu uaaugacaca gggcaugaaa     1080

ccgaugagaa ccgggcuaag guggagauua cgcccaauuc ucccagagcg gaagccacac     1140

uuggaggcuu cggcagccug gggcucgacu gugagccucg caccggacug gauuuuuccg     1200

accuuuauua ccugaccaug aacaauaaac acuggcucgu ccauaaggaa ugguuccacg     1260

auaucccccu gccuuggcac gcuggcgccg acacagggac cccgcauugg aacaauaaag     1320

aggcccuggu ggaauuuaag gaugcucacg ccaaacggca gacagucgug guucucggau     1380

cucaggaggg cgcggugcau acggcucugg ccggggcccu ugaggcugaa auggacggcg     1440

ccaagggaag acugagcucc ggccaccuca aaugccgccu gaagauggau aagcugcggc     1500

ugaaaggggu cagcuauucu cucuguaccg cugccuucac auuuaccaag auuccggcgg     1560

agacacugca uggaaccgug acgguugaag ugcaguacgc cggcaccgac gggccuugca     1620

aaguccccgc ucaaauggcc guggacaugc agacacugac cccuguggga agacucauca     1680

cagcuaaccc cguuauuacc gaguccacgg aaaacagcaa gaugaugcug gagcuugauc     1740

ccccuuucgg cgacucuuac aucgucaucg gggugggcga gaaaaagauu acacaccacu     1800

ggcaucgcuc cggaagcacc aucggcaagg ccuuugaagc gacagugaga ggggccaaac     1860

ggauggcugu ccugggagau accgccuggg acuucggcuc cguuggggga gcucucaauu     1920

cucugggcaa ggggauucac cagaucuucg gcgccgccuu uaaaagccug uucggaggca     1980

uguccugguu uucucaaauu cuuaucggga cccuccugau guggcuggga cucaacacaa     2040

agaauggcag caucucccug augugucuug cucuuggggg agugcucauu uuccugagca     2100

cggcgguguc ugccugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2160

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2220

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2280

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2400

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2446


<210>  6832
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 2 opt

<400>  6832
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uauugagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauc uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga uuacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagauuccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

ucaccgcuaa ccccguuauu acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacccacc     1800

acuggcaucg cuccggaagc acaaucggca aggccuuuga agcgaccgug agaggggccc     1860

ggcgcauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaagggauu caccagaucu ucggcgccgc cuuuaagagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacacuccu gauguggcug ggacucaaca     2040

cgaaaaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc auuuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6833
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 2 opt

<400>  6833
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauguu ucgcaacccu ggcuucgcuc uggccgcggc cgcuauugcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ccugcucauu gcucccgccu      600

acuccaucag augcauuggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cguuauggcg caggacaagc      720

cuacagugga uaucgagcuu gugaccacca cagucuccaa cauggccgag gugcgcagcu      780

acugcuauga agcuagcauu uccgacaugg ccagcgauuc cagauguccc acgcaagggg      840

aggcuuaccu ggacaagcag ucugacaccc aguauguuug caaacggaca cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugccugcuc caagaaaaug acagggaagu cuauccagcc ugaaaaccuc gaguacagaa     1020

uuaugcuguc ugugcacgga ucccaacaca gcggcaugau cguuaaugac accgggcaug     1080

aaacggauga gaaccgggcu aagguggaga ucacacccaa uucuccgaga gcggaagcca     1140

cccuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugaca augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgcuggcg ccgacaccgg gacaccccau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacgguc gugguucucg     1380

gaucucagga gggcgcggug cauaccgcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua cagcugccuu caccuuuaca aagaucccgg     1560

cggagacccu gcauggaacg gugaccguug aagugcagua cgccggcaca gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacccu gacaccugug ggaagacuca     1680

uuaccgcuaa ccccguuauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacauuguca ucgggguggg cgagaaaaag aucacccacc     1800

acuggcaucg cuccggaagc acaauuggca aggccuuuga agcgaccgug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccgucggg ggagcucuca     1920

auucucuggg caaggggauc caccagauuu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auccuuauug ggacacuccu gauguggcug ggacucaaca     2040

cgaagaaugg cagcaucucc cugauguguc uugcucuugg gggaguucuc aucuuccuga     2100

gcaccgcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6834
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 2 opt

<400>  6834
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caugaccucc acaugggucg uguaugggac cugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuacgc uucccagcca uuccacccgg aaacugcaaa      420

cacgcucuca gaccuggcuc gagagcagag aguacacaaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac cucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

cgugggucga uguggugcug gaacauggag gcugugucac aguuauggcg caggacaagc      720

cuaccgugga uauugagcuu gugacaacca ccgucuccaa cauggccgag gugcgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acacaagggg      840

aggcuuaccu ggacaagcag ucugacacgc aguauguuug caaacggacc cucguggauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggucaca ugcgccaaau      960

uugccugcuc caagaaaaug accggaaaga gcauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc ugugcacggg ucccaacaca gcggcaugau cguuaaugac acagggcaug     1080

aaaccgauga gaaccgggcu aagguggaga uuacgcccaa uucucccaga gcggaagcca     1140

cacuuggagg cuucggcagc cuggggcucg acugugagcc ucgcaccgga cuggauuuuu     1200

ccgaccuuua uuaccugacc augaacaaua aacacuggcu cguccauaag gaaugguucc     1260

acgauauccc ccugccuugg cacgcuggcg ccgacacagg gaccccgcau uggaacaaua     1320

aagaggcccu gguggaauuu aaggaugcuc acgccaaacg gcagacaguc gugguucucg     1380

gaucucagga gggcgcggug cauacggcuc uggccggggc ccuugaggcu gaaauggacg     1440

gcgccaaggg aagacugagc uccggccacc ucaaaugccg ccugaagaug gauaagcugc     1500

ggcugaaagg ggucagcuau ucucucugua ccgcugccuu cacauuuacc aagauuccgg     1560

cggagacacu gcauggaacc gugacgguug aagugcagua cgccggcacc gacgggccuu     1620

gcaaaguccc cgcucaaaug gccguggaca ugcagacacu gaccccugug ggaagacuca     1680

ucacagcuaa ccccguuauu accgagucca cggaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacaucguca ucgggguggg cgagaaaaag auuacacacc     1800

acuggcaucg cuccggaagc accaucggca aggccuuuga agcgacagug agaggggcca     1860

aacggauggc uguccuggga gauaccgccu gggacuucgg cuccguuggg ggagcucuca     1920

auucucuggg caaggggauu caccagaucu ucggcgccgc cuuuaaaagc cuguucggag     1980

gcauguccug guuuucucaa auucuuaucg ggacccuccu gauguggcug ggacucaaca     2040

caaagaaugg cagcaucucc cugauguguc uugcucuugg gggagugcuc auuuuccuga     2100

gcacggcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6835
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 2 opt

<400>  6835
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccgagguu accagacggg      120

gauccgcgua cuauauguac cuugaccgca acgaugcugg cgaagccauu ucuuucccca      180

caacccuggg gaugaauaag ugcuauaucc agauuaugga ccucggacac augugugaug      240

ccacaaugag cuacgagugc ccuaugcugg acgaaggcgu ggagccggau gacguggauu      300

guuggugcaa caccacaucc accugggucg uguaugggac gugucaucac aaaaagggag      360

aagcuagacg gucucgcaga gccguuaccc uucccagcca uuccacacgg aaacugcaaa      420

cccgcucuca gacauggcuc gagagcagag aguacaccaa gcaccugauc cggguggaaa      480

auuggauuuu ucgcaacccu ggcuucgcuc uggccgcggc cgcuaucgcc uggcuccugg      540

gcuccagcac gucucagaaa gucaucuauc uugugaugau ucugcucauc gcucccgccu      600

acuccauuag augcaucggg guuagcaauc gggacuuugu ggagggaaug ucuggcggga      660

caugggucga uguggugcug gaacauggag gcugugucac cgcgauggcc caggacaagc      720

cuacaguuga uauugagcuu gugaccacca caguguccaa cauggcugag guccgcagcu      780

acugcuauga agccucuauc uccgacaugg cuagcgauuc cagauguccc acgcaagggg      840

aggccuaccu ggacaagcag ucugacaccc aguaugugug caaacggaca cucguugauc      900

gcggaugggg caauggcugu gggcuguucg gaaagggcag ccuggugacc ugcgccaaau      960

uugcuugcuc caagaaaaug acagggaagu cuauucagcc ugaaaaccuc gaguacagaa     1020

ucaugcuguc uguccacgga ucccaacaca gcggcaugcu ugugaaugac accgggcaug     1080

aaacggauga gaaccgggcg aagguugaga ucacaccgaa uagccccaga gccgaagcua     1140

cccugggagg cuucggcagc cucgggcugg acugugagcc ucgcaccgga cuugauuuuu     1200

ccgaccugua uuaccucaca augaacaaua aacacuggcu ggcccauaag gaaugguucc     1260

acgauauucc ccugccuugg cacgccggcg cugccaccgg gacaccccau uggaacaaua     1320

aagaggcgcu cguggaauuu aaggacgcuc acgccaaacg gcagacgguc gugguccugg     1380

gaucucagga gggcgccgug cauaccgcuc uugccggggc ucuggaggcc gaaauggaug     1440

gcgcgaaggg aagacucagc uccggccacc ugaaaugccg ccuuaagaug gacaagcugc     1500

ggcucaaagg gguuagcuau ucucugugua cagccgcuuu caccuuuaca aagaucccgg     1560

ccgagaccgu ggauggaacg gucaccgugg aaggccagua cgggggaaca gacggcccuu     1620

gcaaaguucc cgcucaaaug gccguggaca ugcagacccu gacaccuguc gggagacuca     1680

uuaccgcgaa ccccgugauc acggagucca cagaaaacag caagaugaug cuggagcuug     1740

aucccccuuu cggcgacucu uacauuguga ucggaguugg cgagaaaaag aucacccauc     1800

acuggcaccg cuccgggagc acaauuggaa aggccuuuga agcuaccguc cggggcgcca     1860

aaagaauggc ugugcugggg gauaccgccu gggacuucgg auccgugggc ggggcccuca     1920

auucucuggg caagggaauc caucagauua ucggcgcugc guucaaaagc cuguuugggg     1980

gaauguccug guucucucaa auucuuaucg gcacacuccu gauguggcug gggcucaaca     2040

cgaagaaugg aagcaucucc cugauguguc uugcccuggg cgggguccuc auuuuucuga     2100

gcaccgcugu gucuggcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6836
<211>  2440
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 2 opt

<400>  6836
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacgcuag caucgggauu gugucccugc uccugaccac agucauggcc gcggagauca      120

cgagacgggg aucugcuuac uauauguacc uugaucgcag cgacgccggc aaggccauuu      180

ccuucguuac cacacugggg gugaacaaau gccacgugca gaucauggau cucggacaua      240

ugugugacgc uaccaugucu uaugaaugcc ccaugcugga ugagggcguc gaacccgacg      300

auguggacug uuggugcaau acaaccagca cauggguugu guacgggacc ugucaccaua      360

agaaaggaga ggccagacgg ucccgcagag cugucacgcu uccgucucac agcacccgga      420

agcugcaaac acgcucccag accuggcucg aaagcagaga guauacaaaa caucugauua      480

agguggagaa cuggaucuuu cggaaucccg gcuucgcccu gguugcggug gccaucgcuu      540

ggcuccuggg aucuuccacc agccagaagg ucauuuaccu ugugaugauc cugcucauug      600

ccccugcuua uucuauccgc ugcauugggg uguccaacag agauuuuguc gaaggaauga      660

gcggcgggac guggguugac guggugcugg agcacggagg cugugucaca gugauggccc      720

aggauaaacc caccguugac aucgaacuug ugacaaccac cgucucuaau auggcggagg      780

ugcgguccua cugcuacgag gccagcauuu ccgauauggc uucugacagc cgcuguccua      840

cacaagggga agccuaucug gacaagcagu ccgauacgca guacguuugc aaaagaaccc      900

ucguggaccg gggauggggc aacggcugug ggcuguucgg aaagggcagc cuggucacau      960

gcgcuaaauu uaccugcucu aagaagauga cagggaaauc cauccagccc gagaaucucg     1020

aauaucgcau caugcugagc gugcacggau cucaacauag cggcaugauu gucaacgaug     1080

agaauagagc caagguggaa guuaccccua acuccccgcg ggccgaggcu acgcuugggg     1140

gauucggcuc ucugggccuc gacugugagc ccagaacagg gcuggauuuu agcgaccuuu     1200

acuaucugac caugaauaac aaacacuggc ucgugcauaa ggaaugguuc cacgauaucc     1260

cucugcccug gcacuccgga gcggacaccg agacaccuca uuggaauaac aaagaagccc     1320

uggucgaguu uaaggaugcu cacgccaaac gccagaccgu gguugugcuc ggcagccagg     1380

aaggggccgu ccauacagcu cuggccggag cgcuugaggc ugagauggac ggcgccaagg     1440

ggcggcuguc uuccggccac cucaagugca gacugaaaau ggauaaacuu cgccugaagg     1500

gagugagcua cucucucugu acggccgcuu ucaccuuuac aaaggugccc gccgaaaccc     1560

ugcauggcac aguuaccguc gaggugcagu augcugggcg ggacggaccg ugcaaagugc     1620

cugcccaaau ggcggucgac augcagacgc ugacccccgu gggcagacuc auuacagcca     1680

auccuguuau caccgaaucc acagagaaca gcaagaugau gcuggaacuu gauccucccu     1740

ucggggacuc cuacauugug aucggagucg gcgauaagaa aaucacccac cacuggcauc     1800

gcucugggag cauuaucggc aaggcuuuug aggccacggu gcggggagcu aaaagaaugg     1860

ccguucuggg cgacacagcg ugggauuucg gguccguggg aggcgucuuc aacucucucg     1920

ggaagggcau ucaccagauc uuuggcgccg cuuucaaaag ccuguuuggg ggcauguccu     1980

gguucagcca aauucugauc ggaacccuuc ucguguggcu gggccugaau acaaagaacg     2040

ggucuaucuc ccucaccugu cuggcccuug gaggcguuau gauuuuucug agcaccgcug     2100

ugucugccug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2160

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2220

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2280

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2400

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2440


<210>  6837
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 2 opt

<400>  6837
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacaccag caucgggauu aucggacugc uccugacaac ggcuauggcc gcggagauua      120

ccagacgggg cuccgcuuac uauauguacc uugaucgcuc ugacgccggg aaggccauca      180

gcuucgcuac aaccauggga gugaacaaau gccacgucca gauuauggau cugggccaua      240

ugugugacgc cacaaugucc uaugaaugcc ccaugcucga ugaggggguu gaaccugacg      300

auguggacug uuggugcaau accacaucua ccuggguggu cuacggaacg ugucaccaua      360

agaaaggcga ggcuagaccg agccggcgcg ccgugacccu gcccucccac ucuacaagaa      420

aacuucaaac ccggagccag acauggcugg aaucccgcga guauaccaag caucucauca      480

agguugagaa cuggauccug agaaauccug gcuuuacggu ggcggcccug gcuauggccu      540

ggcuccuggg gaacagcaca ucucagcggg ucauuuaccu ugugaugauc cugcucauug      600

cucccgccua uuccauccgc ugcauuggag uuagcaauag agauuucgug gaaggcaugu      660

cugggggaac cugggucgac guggugcugg agcacggcgg gugugucaca guuauggcgc      720

aggauaagcc uaccguggac aucgaacuug ugaccacaac ggucuccaac auggccgagg      780

ugcggagcua cugcuacgag gcuucuauuu ccgauauggc cagcgacucc cgcuguccca      840

cccaaggaga ggcuuaucug gacaaacagu cugauacaca guacguuugc aagagaaccc      900

ucguggaccg gggcuggggc aaugggugug gacuguuugg caaagggagc cuggucacau      960

gcgccaaguu uaccugcucc aaaaagauga cgggaaagag cauccagccu gaaaaccucg     1020

aauaucgcau caugcugucu gugcacggcu cccaacauag cgggaugauu guuaaugaua     1080

ucggacacga gacagacgaa aacagagcca aaguggaggu cacccccaau ucuccccggg     1140

cugaggcgac ccuuggcggc uucgggagcc ugggacucga uugugaaccu agaacaggcc     1200

uggacuucuc cgaucuuuac uaucugacca ugaacaauaa gcauuggcuc gugcacaaag     1260

agugguuuca cgacauuccc cugccuuggc augccggggc ugauacagga acgccccacu     1320

ggaacaauaa ggaagcccug gucgaguuca aagacgccca ugcuaagcgc cagaccgugg     1380

uugugcucgg cucucaggaa ggggccgucc acacagcgcu ggcuggcgcc cuugaggccg     1440

agauggaugg agcuaaaggc cggcuguuua gcgggcaucu caagugcaga aucaagaugg     1500

acaaacugcg ccuuaaggga guguccuaca gccuguguac cgccgcuuuc acauuuucua     1560

aaguuccggc cgaaacccuc cacggcacgg ugaccgucga ggugcaguau gcggggcggg     1620

acggaccuug caaggugccc gcccaaaugg cuguugauau gcagacacug accccugucg     1680

gcagacugau uacagccaac cccaucauca ccgaauccac ggagaacagc cgcaugaugc     1740

ucgaacugga cccgccuuuc ggggauucuu acauugugau cggcguggga gacaaaaaga     1800

uuacacacca uuggcaccgg uccggcagca ccaucgggaa ggcuuuugag gccacaguca     1860

gaggagcgaa acgcauggcc gugcuuggcg auaccgcuug ggacuucggg uccguuggag     1920

gcguguucaa uucucugggg aagggcgucc aucagguguu uggagccgcu uucaaaagcc     1980

ucuuuggcgg gauguccugg uucucucaaa uucugaucgg aacccugcuu guuuggcucg     2040

gccugaacac aaagaauggg agcaucuccc uggccugucu cgcccuggga ggcgcuauga     2100

uuuuucuuag cacggcggug ucugccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6838
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 2 opt

<400>  6838
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggauauug      180

uccuugaaca cgggggaugu gugacaguua uggcccaaga caaaccuacg guggauaucg      240

agcuggucac cacaaccgug uccaauaugg cggaaguucg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gugugcaaac ggacacuggu cgaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauu caacccgaga aucucgagua cagaaucaug cugagcgugc      540

augggucuca gcacuccgga augauuguca acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ugaaaucacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac auuccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggugg      840

aguucaagga ugcucacgcc aagcggcaga cagucguggu ucucgggagc caggaaggag      900

cggugcauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguca     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ccccgcggag acccuucacg     1080

ggacagugac cguugaagug caauaugccg gaacagacgg cccuuguaaa guccccgcuc     1140

agauggccgu ggauaugcag acccugacgc cugucgggcg ccucauuaca gccaaucccg     1200

ugaucaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cguuauuggc gugggggaaa agaaaaucac acaucacugg cauagaagcg     1320

gcucuaccau uggaaaggcu uucgaggcca caguccgggg cgcgaaacgc auggcugugc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6839
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 2 opt

<400>  6839
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6840
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 2 opt

<400>  6840
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucggcu augaaacaga ugaggaccgg gccaagguug      600

aagugacccc uaacagcccc cgcgcugagg ccacacucgg gggauuuggc ucucugggcc      660

uggauugcga gccuagaacc gggcuugacu ucuccgaucu guacuaccuc acgaugaaua      720

acaaacauug gcuggugcac aaggaauggu uucaugacau uccccuuccu uggcacgcgg      780

gagccgacac aggcaccccg cacuggaaua acaaagaggc ucuggucgag uucaaggaug      840

cccaugcuaa gcggcagaca gugguugugc ucgggagcca ggaaggagcc guccacaccg      900

cgcuggccgg cgcucuggaa gccgagaugg acggggcuaa aggacgccuc uuuuccggcc      960

aucugaagug uagacuuaaa auggauaagc ugcggcucaa aggcgugucu uauagccugu     1020

gcaccgccgc cuucacauuu acgaagguuc ccgcugagac ccuucacggg acagugaccg     1080

ucgaagugca auacgcggga acagacggcc cuuguaaaau ccccguccag auggccgugg     1140

auaugcagac ccugacgccu guugggcgcc ucauuacagc uaaucccgug aucaccgagu     1200

ccaccgaaaa cagcaagaug augcuggagc uggacccgcc uuucggagau ucuuauauug     1260

ucaucggcgu gggggacaag aaaaucacac accauuggca cagauccggc agcaccauug     1320

gaaaggccuu ugaagccaca guucggggcg cuaaacgcau ggccgugcuc ggggauacgg     1380

cgugggacuu cggaucuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6841
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 2 opt

<400>  6841
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6842
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 2 opt

<400>  6842
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauacagg cuaugaaacc gacgagaauc      600

gggccaaggu ggaagugaca ccuaacagcc cccgcgcuga ggccacccuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa cggggcuuga cuucuccgau cuguacuacc      720

ucacaaugaa uaacaaacau uggcuggucc acaaggaaug guuucaugac auuccccuuc      780

cuuggcacgc gggagccgac accggcacac cgcacuggaa uaacaaagag gcucuggugg      840

aguucaagga ugcccaugcu aagcggcaga ccguuguggu ccucgggagc caggaaggag      900

ccgugcacac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu aaaggaaagc      960

ucuuuuccgg ccaucugaaa ugucgccuua agauggauaa acugagacuc aagggcguuu     1020

cuuauagccu gugcacagcc gccuucacgu uuaccaaagu gcccgcugag acacuucacg     1080

ggaccgucac aguggaaguc caauacgcgg gaaccgacgg cccuuguaag auccccgugc     1140

agauggccgu ugauaugcag acgcugacac cuguggggcg gcucauuacc gcuaaucccg     1200

ucaucaccga guccacagaa aacagcaaga ugaugcugga gcuggacccg ccuuucggag     1260

auucuuauau ugugaucggc guuggggaca aaaagaucac ccaccauugg caccgcuccg     1320

gcagcacaau uggaaaagcc uuugaagcca cggugagagg cgcuaagcgg auggccgucc     1380

ucggggauac cgcgugggac uucggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6843
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 2 opt

<400>  6843
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augacaguua acgauaucgg cuaugaaacc gacgagaauc      600

gggccaaggu ggaagugaca ccuaacagcc cccgcgcuga ggccacccuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa cggggcuuga cuucuccgau cuguacuacc      720

ucacaaugaa uaacaaacau uggcuggucc acaaggaaug guuucaugac auuccccuuc      780

cuuggcacgc gggagccgac accggcacac cgcacuggaa uaacaaagag gcucuggugg      840

aguucaagga ugcccaugcu aagcggcaga ccguuguggu ccucgggagc caggaaggag      900

ccgugcacac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu aaaggaaagc      960

ucuuuuccgg ccaucugaaa ugucgccuua agauggauaa acugagacuc aagggcguuu     1020

cuuauagccu gugcacagcc gccuucacgu uuaccaaagu gcccgcugag acacuucacg     1080

ggaccgucac aguggaaguc caauacgcgg gaaccgacgg cccuuguaag auccccgugc     1140

agauggccgu ugauaugcag acgcugacac cuguggggcg gcucauuacc gcuaaucccg     1200

ucaucaccga guccacagaa aacagcaaga ugaugcugga gcuggacccg ccuuucggag     1260

auucuuauau ugugaucggc guuggggaca aaaagaucac ccaccauugg caccgcuccg     1320

gcagcacaau uggaaaagcc uuugaagcca cggugagagg cgcuaagcgg auggccgucc     1380

ucggggauac cgcgugggac uucggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6844
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 2 opt

<400>  6844
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgaugaaaa ucgggccaag guggagguga      600

caccuaacag cccccgcgcu gaagccaccc ugggcggguu uggaucucuc ggccuggacu      660

gcgagccuag aacaggccuu gauuucuccg accuguauua ccucaccaug aauaacaaac      720

auuggcuggu ccacaaggag ugguuucaug auauuccccu uccuuggcac gcgggggccg      780

acacgggaac accgcacugg aauaacaaag aagcucuggu ggaguucaag gacgcccaug      840

cuaagcggca gaccguugug guccucggca gccaggaagg ggccgugcac acagcgcugg      900

ccggagcucu ggaggccgaa auggauggcg cuaaagggcg ccucuuuucc ggacaucuga      960

aguguagacu uaaaauggac aagcugcggc ucaaaggcgu uucuuacagc cugugcaccg     1020

ccgccuucac cuuuacaaag gugcccgcug agacgcuuca cggcaccguc acaguggagg     1080

uccaauaugc ggggaccgau ggaccuugua aagugcccgc ccagauggcu guugacaugc     1140

agacacugac cccugugggc cgccucauca cggccaaucc cgucauuaca gaauccaccg     1200

agaacagcaa gaugaugcug gaacuggauc cgccuuucgg ggacucuuac aucgugauug     1260

gaguuggcga uaagaaaauc acccaccauu ggcacagauc cgggagcaca aucggcaagg     1320

ccuuugaggc uaccgugcgg ggagccaaac gcauggcggu ccucggcgac acagcuuggg     1380

auuucggguc uugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  6845
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 2 opt

<400>  6845
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucggcu augaaacaga ugagaaccgg gccaagguug      600

aagugacccc uaauagcccc cgcgcugagg ccacacucgg gggauuuggc ucucugggcc      660

uggacugcga gccuagaacc gggcuugauu ucuccgaccu guacuaccuc acgaugaaca      720

auaaacauug gcuggugcac aaggaauggu uucaugauau uccccuuccu uggcacgcgg      780

gagccgacac aggcaccccg cacuggaaca auaaagaggc ucuggucgag uucaaggacg      840

cccaugcuaa gcggcagaca gugguugugc ucgggagcca ggaaggagcc guccacaccg      900

cgcuggccgg cgcucuggaa gccgagaugg auggggcuaa aggaaagcuc uuuuccggcc      960

aucugaaaug ucgccuuaag auggacaaac ugagacucaa gggcgugucu uauagccugu     1020

gcaccgccgc cuucacauuu acgaaaguuc ccgcugagac ccuucacggg acagugaccg     1080

ucgaagugca auacgcggga acagauggcc cuuguaagau ccccguccag auggccgugg     1140

acaugcagac ccugacgccu guugggcggc ucauuacagc uaaccccgug aucaccgagu     1200

ccaccgaaaa uagcaagaug augcuggagc uggauccgcc uuucggagac ucuuauauug     1260

ucaucggcgu gggggauaaa aagaucacac accauuggca ccgcuccggc agcaccauug     1320

gaaaagccuu ugaagccaca guuagaggcg cuaagcggau ggccgugcuc ggggacacgg     1380

cgugggauuu cggaucuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6846
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 2 opt

<400>  6846
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc cuuagaugca      120

uuggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuggaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauaucg      240

agcucgugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcauuuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccuggaca      360

agcaguccga uacccaguau gucugcaaac ggacacuugu ggaccgcggg uggggaaacg      420

gcuguggccu guuugggaag ggaagccucg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucuggagua cagaaucaug cugagcgucc      540

augggucuca gcacuccgga augauuguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaaucacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuugauugc gagccuagaa ccgggcugga cuucuccgau cucuauuacc      720

ugacgaugaa uaacaaacac uggcuugugc auaaggaaug guuucacgac auuccccugc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccucgucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcuggggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccucg aagcugagau ggacggggcc aaaggacgcc      960

uguccucugg ccaccuuaag uguagacuga aaauggauaa gcuccggcug aaaggcguga     1020

gcuacucccu uugcaccgcc gcuuuuacau ucacgaagau ccccgcggag acccugcacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccucacgc cuguggggcg ccugauuaca gccaaucccg     1200

uuaucaccga gagcaccgaa aacucuaaga ugaugcugga gcucgacccg ccuuuuggag     1260

auuccuacau ugugaucggc gucggggaaa agaaaaucac acaucacugg cauagaagcg     1320

gcucuaccau uggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ugggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6847
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 2 opt

<400>  6847
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagca       60

agaaagugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccagga caagccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca aggcgaagcc uaccucgaca      360

aacaguccga uacccaguau gucugcaagc ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaaa ggaagccugg ugaccugcgc uaaguucgcc uguucuaaga      480

aaaugacggg caaguccauc cagcccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca acacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaagu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaagcac uggcuggugc auaaagaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaggag gcccuggucg      840

aguucaagga ugcucacgcc aaacggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aagggacgcc      960

ucuccucugg ccaccugaaa uguagacuua agauggauaa acugcggcuc aagggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaaaau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caguaugccg gaacagacgg cccuuguaag gugcccgcuc     1140

aaauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaga aaaagauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaagcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6848
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 2 opt

<400>  6848
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg uauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6849
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 2 opt

<400>  6849
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggaaagc      960

ucuccucugg ccaccugaaa ugucgccuua agauggauaa acugagacuc aagggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaaaau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaag gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg gcucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaga aaaagauuac acaucacugg caucgcagcg     1320

gcucuaccau cggaaaggcu uucgaagcca cagugagagg cgcgaaacgg auggcuguuc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6850
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 2 opt

<400>  6850
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauacccag acgcugacac cuguggggcg ccucaucacc gccaaucccg     1200

uuauuaccga gagcacagaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaagcg     1320

gcucuacaau cggaaaggcu uucgaggcca cggugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac cgccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6851
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 2 opt

<400>  6851
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaaauccg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gugugcaaac ggacacuggu cgaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauu caacccgaga aucucgagua cagaaucaug cugagcgugc      540

augggucuca gcacuccgga augauuguca acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ugaaaucacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac auuccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggugg      840

aguucaagga ugcucacgcc aagcggcaga cagucguggu ucucgggagc caggaaggag      900

cggugcauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguca     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ccccgcggag acccuucacg     1080

ggacagugac cguugaagug caauaugccg gaacagacgg cccuuguaaa guccccgcuc     1140

agauggccgu ggauaugcag acccugacgc cugucgggcg ccucauuaca gccaaucccg     1200

ugaucaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cguuauuggc gugggggaaa agaaaaucac acaucacugg cauagaagcg     1320

gcucuaccau uggaaaggcu uucgaggcca caguccgggg cgcgaaacgc auggcugugc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6852
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 2 opt

<400>  6852
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgcgcaga auggcuguuc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6853
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 2 opt

<400>  6853
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggucucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gugugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg uuaccugcgc caaauucgcu uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguga acgauaccgg ccaugaaaca gacgagaauc      600

gggccaaggu ggaaauuacc ccuaacagcc cccgcgcuga ggccacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggucc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc gggagccgac acaggcaccc cgcauuggaa uaacaaagag gcucuggugg      840

aguucaagga ugcccacgcu aagcggcaga caguuguggu ccucgggagc caggaaggag      900

ccgugcauac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguua     1020

gcuacucccu gugcaccgcc gccuuuacau ucacgaagau ucccgcugag acccuucacg     1080

ggacagugac cgucgaagug caauaugcgg gaacagacgg cccuuguaaa guccccgccc     1140

agauggcugu ggauaugcag acccugacgc cuguugggcg ccucaucaca gccaaucccg     1200

ugauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgucaucggc gugggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcc uucgaggcua caguucgggg cgccaaacgc auggcggugc     1380

ucggggacac ggcuugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6854
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 2 opt

<400>  6854
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaucgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugauuggc gucggggaaa agaaaaucac ccaucacugg cauagaagcg     1320

gcucuacaau uggaaaggcu uucgaggcca ccgugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac agccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6855
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 2 opt

<400>  6855
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc auuggggaaa agaaaaucac acaucacugg cauagaagcg     1320

gcucuaccau uggaaaggcu uucgaggcca caguccgggg cgcgaaacgc auggcugugc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6856
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 2 opt

<400>  6856
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccu ugcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuggaaca cgggggaugu guuacaguga uggcccaaga caaacccacg gucgauauug      240

agcucgugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccuacaca gggcgaagcc uaccuggaca      360

agcaguccga uacccaguau gucugcaaac ggacacuugu ggaccgcggg uggggaaacg      420

gcuguggccu guuugggaag ggaagccucg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caaccggaga aucuggagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc cccaacagcc cucgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuugauugc gagcccagaa ccgggcugga cuucuccgau cucuauuacc      720

ugacgaugaa uaacaaacac uggcuugugc auaaggaaug guuucacgac aucccucugc      780

ccuggcacgc cggagcugac acaggcaccc cucauuggaa uaacaaagag gcccucgucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcuggggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccucg aagcugagau ggacggggcc aaaggacgcc      960

uguccucugg ccaccuuaag uguagacuga aaauggauaa gcuccggcug aaaggcguga     1020

gcuacucccu uugcaccgcc gcuuuuacau ucacgaagau uccggcggag acccugcacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg ccccuguaaa gugccugcuc     1140

agauggccgu cgauaugcag acccucacgc ccguggggcg ccugaucaca gccaauccug     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcucgauccc ccguuuggag     1260

acuccuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ugggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6857
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 2 opt

<400>  6857
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggacc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucagcuccgg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcgugu     1020

cuuacagccu gugcacagcc gcuuuuacgu ucaccaagau ucccgcggag acacuucacg     1080

ggaccguuac aguggaaguc caauaugccg gaaccgacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acgcugacac cuguggggcg ccucaucacc gccaaucccg     1200

uuauuaccga guccacagaa aacagcaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auucuuacau cgugaucggc gucggggaaa agaaaauuac ccaucacugg cauagauccg     1320

gcagcacaau cggaaaggcu uucgaggcca cggugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac cgccugggau uuuggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6858
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 2 opt

<400>  6858
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcuggcuc     1140

agauggccgu cgauaugcag acccucacgc ccguggggcg ccugaucaca gccaauccug     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcucgauccc ccguuuggag     1260

acuccuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ugggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6859
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 2 opt

<400>  6859
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga agcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aggcugagau ggauggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggacaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggaa acccuucacg     1080

ggacaguuac cguggagguc caauaugccg gaacagaugg cccuuguaaa gugcccgcuc     1140

agauggccgu cgacaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga aagcaccgag aacucuaaga ugaugcugga acuggauccg ccuuuuggag     1260

acuccuacau cgugaucggc gucggggaga agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggauac ggccugggac uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6860
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 2 opt

<400>  6860
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcggcccuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa cagggcuuga cuucuccgau cuguauuacc      720

ucaccaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc uggagccgac acgggcacac cgcauuggaa uaacaaagag gcucuggucg      840

aguucaagga ugcccacgcg aagcggcaga ccgugguugu gcucgggagc caggaaggag      900

ccguccauac agcucuggcc ggcgcucugg aagccgagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcu gcguuuaccu ucacaaagau ucccgccgag acgcuucacg     1080

ggaccguuac aguggaaguc caauaugcug gaaccgacgg cccuuguaaa gugcccgccc     1140

agauggccgu cgauaugcag acacugaccc cuguggggcg ccucaucacg gcuaaucccg     1200

uuauuacaga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaagcg     1320

gcucuacaau cggaaaggcc uucgaggcga ccgugcgggg cgcuaaacgc auggccguuc     1380

ucggggacac agccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6861
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 2 opt

<400>  6861
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcugguguc caccacaguu ucuaauaugg cggaagugcg cagcuacugc uaugaggcua      300

ccaucuccga caucgccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauu caacccgaga aucucgagua cagaaucaug cugagcgucc      540

augggucuca gcacuccgga augauuguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaaucacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac auuccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagcgguggu ucucgggagc caggaaggag      900

ccgugcauac cgcucuggcc ggcgcucugg aagccgagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguca     1020

gcuacucccu gugcaccgcu gcguuuacau ucacgaagau ccccgccgag acccuucacg     1080

ggacagugac cguugaagug caauaugcug gaacagacgg cccuuguaaa guccccgccc     1140

agauggccgu ggauaugcag acccugacgc cugucgggcg ccucauuaca gcuaaucccg     1200

ugaucaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cguuauuggc gugggggaaa agaaaaucac acaucacugg cauagaagcg     1320

gcucuaccau uggaaaggcc uucgaggcga caguccgggg cgcuaaacgc auggccgugc     1380

ucggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6862
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 2 opt

<400>  6862
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucagcaagau ucccgcggag acgcuucacg     1080

ggaccguuac agaagaggug caauaugccg gaaccgacgg cccuuguaaa guccccgcuc     1140

agauggccgu ggauaugcag acacugaccc cugucgggcg ccucaucacg gccaaucccg     1200

ugauuacaga aucuaccgag aacuccaaga ugaugcugga acuggacccg ccuuuuggag     1260

auagcuacau cguuaucggc gugggggaga agaaaauuac ccaucacugg cauagaucug     1320

gcagcacaau cggaaaggcu uucgaggcca ccguccgggg cgcgaaacgc auggcugugc     1380

ucggggacac agccugggau uuuggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6863
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 2 opt

<400>  6863
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaacug caauaugccg gaacagacgg cccuuguaaa guccccgcuc     1140

agauggccgu ggauaugcag acccucacgc cugucgggcg ccugaucaca gccaaucccg     1200

ugauuaccga gagcaccgaa aacucuaaga ugaugcugga gcucgacccg ccuuuuggag     1260

auuccuacau cguuaucggc gugggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcu uucgaggcca caguccgggg cgcgaaacgc auggcugugc     1380

ugggggacac ggccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6864
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 2 opt

<400>  6864
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcauuuc uaaccgggac uucguugagg ggaugagcgg aggcaccugg guggaugugg      180

uccuugaaca cgggggaugu gugacaguua uggcccaaga caaaccuacg guggauaucg      240

agcuggucac cacaaccgug uccaauaugg cggaaguucg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gugugcaaac ggacacuggu cgaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauu caacccgaga aucucgagua cagaaucaug cugagcgugc      540

augggucuca gcacuccgga augauuguca acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ugaaaucacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcauucuggg ccuggauugc gagccuagaa ccgggcuuga cuucucugau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggugg      840

aguucaagga ugcucacgcc aagcggcaga cagucguggu ucucgggucc caggaaggag      900

cggugcauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucagcuccgg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcgucu     1020

cuuacagccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacagugac cguugaagug caauaugccg gaacagacgg cccuuguaaa guccccgcuc     1140

agauggccgu ggauaugcag acccugacgc cugucgggcg ccucaucaca gccaaucccg     1200

ugaucaccga guccaccgaa aacagcaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auucuuacau uguuaucggc gugggggaaa agaaaauuac acaucacugg cauagauccg     1320

gcagcaccau cggaaaggcu uucgaggcca caguccgggg cgcgaaacgc auggcugugc     1380

ucggggacac ggccugggau uuuggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6865
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 2 opt

<400>  6865
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggug aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguua     1020

gcuacucccu gugcaccgcc gccuuuacau ucacgaagau ucccgcugag acccuucacg     1080

ggacagugac cgucgaagug caauaugcgg gaacagacgg cccuuguaaa guccccgccc     1140

agauggcugu ggauaugcag acccugacgc cuguugggcg ccucaucaca gccaaucccg     1200

ugauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgucaucggc gugggggaaa agaaaauuac acaucacugg cauagaagcg     1320

gcucuaccau cggaaaggcc uucgaggcua caguucgggg cgccaaacgc auggcggugc     1380

ucggggacac ggcuugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6866
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 2 opt

<400>  6866
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gagcugacgg cccuuguaaa gugcccgccc     1140

agauggccgu cgauaugcag acacugaccc cuguggggcg ccucaucacg gcuaaucccg     1200

uuauuacaga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaagcg     1320

gcucuacaau cggaaaggcc uucgaggcga ccgugcgggg cgcuaaacgc auggccguuc     1380

ucggggacac agccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6867
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 2 opt

<400>  6867
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacac aggagccgac accggcacac cgcauuggaa uaacaaagag gcucuggucg      840

aguucaagga ugcccacgcu aagcggcaga ccgugguugu gcucgggagc caggaaggag      900

ccguccauac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcacagcc gccuuuacgu ucaccaagau ucccgcugag acacuucacg     1080

ggaccguuac aguggaaguc caauaugcgg gaaccgacgg cccuuguaaa gugcccgccc     1140

agauggcugu cgauaugcag acgcugacac cuguggggcg ccucaucacc gccaaucccg     1200

uuauuaccga gggaacagaa aacagcaaga ugaugcugga gcuggacccg ccuuuuggcg     1260

auucuuacau cgugaucggg gucggcgaaa agaaaauuac ccaucacugg cauagauccg     1320

gaagcacaau cggcaaggcc uucgaggcua cggugcgcgg ggccaaacgg auggcgguuc     1380

ucggagacac cgcuugggau uuuggcucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6868
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 2 opt

<400>  6868
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacagcca uggcgcaaga caaaccuacg guggauauug      240

agcuggucac cacaaccgug uccaauaugg cugaaguucg cucuuacugc uaugaggcca      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaggcu uaccucgaca      360

agcaguccga uacccaguau gugugcaaac ggacacuggu cgaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc caaauucgcu uguucuaaga      480

agaugacggg caaauccauc caacccgaaa aucucgagua cagaauuaug cugagcgugc      540

augggucuca gcacuccgga augcugguca acgauaccgg ccaugaaaca gacgagaauc      600

gggccaaggu ugaaaucacc ccuaacagcc cccgcgcgga ggccacacuc gggggauuug      660

gcucucuggg ccuugauugc gagccuagaa ccgggcugga cuucuccgau cucuauuacc      720

ugacgaugaa uaacaaacac uggcuugcuc auaaggaaug guuucacgac auuccccugc      780

cuuggcacgc cggagcugcc acaggcaccc cgcauuggaa uaacaaagag gcgcucgugg      840

aguucaagga cgcccacgcu aagcggcaga caguggucgu gcuggggagc caggaaggag      900

ccguucauac cgcucuggcc ggcgcccucg aagcugagau ggauggggcg aaaggacgcc      960

uguccucugg ccaccuuaag uguagacuga aaauggacaa gcuccggcug aaaggcguga     1020

gcuacucccu uugcaccgcc gcuuuuacau ucacgaagau ccccgccgag accgucgaug     1080

ggacagugac cguugaagga caauauggcg ggacagacgg accuuguaaa gugcccgccc     1140

agauggcugu cgauaugcag acccugacgc cugugggccg ccucauuaca gccaaucccg     1200

ucaucaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuugggg     1260

auuccuacau ugugaucggc guuggagaaa agaaaaucac acaccauugg cacagaagcg     1320

gcucuaccau ugggaaggcg uucgaggcua cagugcgggg agccaaacgc auggccgucc     1380

ucggcgacac ggcuugggau uuugggagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6869
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 2 opt

<400>  6869
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccugcg gucgauauug      240

agcuggugac gaccacaguu uccaauaugg cugaagugcg cucuuacugc uaugaggcca      300

gcaucuccga cauggccucu gauagcagau guccgaccca gggcgaggcu uaccucgaca      360

agcaguccga uacacaguau gucugcaaac ggacccuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugacaugcgc caaauucgcu uguucuaaga      480

agaugaccgg caaauccauc caacccgaaa aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauacggg ccaugaaacc gacgagaauc      600

gggccaaggu ggaaauuaca ccuaacagcc cccgcgcgga ggccacccuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa cagggcuuga cuucuccgau cuguauuacc      720

ucaccaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc uggagccgac acgggcacac cgcauuggaa uaacaaagag gcucuggucg      840

aguucaagga ugcccacgcg aagcggcaga ccgugguugu gcucgggagc caggaaggag      900

ccguccauac agcucuggcc ggcgcucugg aagccgagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcu gcguuuaccu ucacaaagau ucccgccgag acgcuucacg     1080

ggaccguuac aguggaaguc caauaugcug gaaccgacgg cccuuguaaa gugcccgccc     1140

agauggccgu cgauaugcag acacugaccc cuguggggcg ccucaucacg gcuaaucccg     1200

uuauuacaga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaagcg     1320

gcucuacaau cggaaaggcc uucgaggcga ccgugcgggg cgcuaaacgc auggccguuc     1380

ucggggacac agccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6870
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 2 opt

<400>  6870
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuaa caaucgggac uucguggagg ggaugucugg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu agcaacaugg cggaagugcg cuccuacugc uaugaggcuu      300

cuaucagcga cauggccucc gauucuagau guccgacaca gggcgaagcc uaccucgaca      360

agcagagcga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaaug      420

gcuguggccu uuuugggaag ggaucccugg ugaccugcgc uaaauucgcc uguagcaaga      480

agaugacggg caaaucuauc caacccgaga accucgagua cagaauuaug cuguccgucc      540

augggagcca gcacucugga augaucguua augauaccgg ccaugaaaca gacgagaacc      600

gggcuaaggu ggaaauuacc ccuaauagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucccuggg ccuggauugc gagccuagaa ccgggcuuga cuucucugau cuguauuacc      720

ucacgaugaa caauaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa caauaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggucc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucagcuccgg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcgugu     1020

cuuacagccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaccccg     1200

uuauuaccga guccaccgaa aauagcaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auucuuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg cauagauccg     1320

gcagcaccau cggaaaggcu uucgaggcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggacac ggccugggau uuuggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6871
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 2 opt

<400>  6871
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcgccgcu gcguuuaccu ucacaaagau ucccgccgag acgcuucacg     1080

ggaccguuac aguggaaguc caauaugcug gaaccgacgg cccuuguaaa gugcccgccc     1140

agauggccgu cgauaugcag acacugaccc cuguggggcg ccucaucacg gcuaaucccg     1200

uuauuacaga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaaa agaaaauuac ccaucacugg cauagaagcg     1320

gcucuacaau cggaaaggcc uucgaggcga ccgugcgggg cgcuaaacgc auggccguuc     1380

ucggggacac agccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6872
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 2 opt

<400>  6872
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauauugg ccaugaaacc gacgagaauc      600

gggcuaaggu ggaaaucaca ccuaacagcc cccgcgccga ggcgacccuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa cagggcuuga cuucuccgau cuguauuacc      720

ucaccaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac auuccccuuc      780

cuuggcacgc cggagcugac acgggcacac cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga ccgugguugu gcucgggagc caggaaggag      900

cgguccauac agcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuaccu ucacaaagau ccccgcggag acgcuucacg     1080

ggaccguuac aguggaaguc caauaugccg gaaccgacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acacugaccc cuguggggcg ccucauuacg gccaaucccg     1200

uuaucacaga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugauuggc gucggggaca agaaaaucac ccaucacugg cauagaagcg     1320

gcucuacaau uggaaaggcu uucgaagcca ccgugcgggg cgcgaaacgc auggcuguuc     1380

ucggggauac agccugggac uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6873
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 2 opt

<400>  6873
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucgcc uguucuaaga      480

agaugacggg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gggcuaaggu ggaaauuacc ccuaacagcc cccgcgccga ggcgacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcggcaga cagugguugu gcucgggagc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggacgcc      960

ucuccucugg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguga     1020

gcuacucccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggcg ccucaucaca gccaaucccg     1200

uuauuaccga gagcaccgaa aacucuaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auuccuacau cgugaucggc gucggggaca agaaaauuac acaucacugg uauagaagcg     1320

gcucuaccau cggaaaggcu uucgaagcca cagugcgggg cgcgaaacgc auggcuguuc     1380

ucggggauac ggccugggac uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6874
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 2 opt

<400>  6874
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauacagg ccaugaaacc gacgagaauc      600

gggccaaggu ggaagugaca ccuaacagcc cccgcgcuga ggccacccuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa cggggcuuga cuucuccgau cuguauuacc      720

ucacaaugaa uaacaaacac uggcuggucc auaaggaaug guuucacgac auuccccuuc      780

cuuggcacgc gggagccgac accggcacac cgcauuggaa uaacaaagag gcucuggugg      840

aguucaagga ugcccacgcu aagcggcaga ccguuguggu ccucgggagc caggaaggag      900

ccgugcauac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu aaaggacgcc      960

ucuuuuccgg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguuu     1020

cuuacagccu gugcacagcc gccuucacgu uuaccaaggu gcccgcugag acacuucacg     1080

ggaccgucac aguggaaguc caauaugcgg gaaccgacgg cccuuguaaa gugcccgccc     1140

agauggcugu ugauaugcag acgcugacac cuguggggcg ccucaucacc gccaaucccg     1200

ucauuaccga guccacagaa aacagcaaga ugaugcugga gcuggacccg ccuuucggag     1260

auucuuacau cgugauuggc guuggggaca agaaaaucac ccaucacugg cauagauccg     1320

gcagcacaau cggaaaggcc uuugaagcua cggugcgggg cgccaaacgc auggcggucc     1380

ucggggauac cgcuugggac uucggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6875
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 2 opt

<400>  6875
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauauugg ccaugaaaca gacgagaauc      600

gggccaaggu ggaagugacc ccuaacagcc cccgcgcuga ggccacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggucc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc gggagccgac acaggcaccc cgcauuggaa uaacaaagag gcucuggugg      840

aguucaagga ugcccacgcu aagcggcaga caguuguggu ccucgggagc caggaaggag      900

ccgugcauac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu aaaggacgcc      960

ucuuuuccgg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguuu     1020

cuuacagccu gugcaccgcc gccuucacau uuacgaaggu gcccgcugag acccuucacg     1080

ggacagucac cguggaaguc caauaugcgg gaacagacgg cccuuguaaa gugcccgccc     1140

agauggcugu ugauaugcag acccugacgc cuguggggcg ccucauuaca gccaaucccg     1200

ucaucaccga guccaccgaa aacagcaaga ugaugcugga gcuggacccg ccuuucggag     1260

auucuuacau ugugaucggc guuggggaca agaaaaucac acaucacugg cauagauccg     1320

gcagcaccau uggaaaggcc uuugaagcua cagugcgggg cgccaaacgc auggcggucc     1380

ucggggauac ggcuugggac uucggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6876
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 2 opt

<400>  6876
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauauugg ccaugaaaca gacgagaauc      600

gggccaaggu ggaagugacc ccuaacagcc cccgcgcuga ggccacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggucc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc gggagccgac acaggcaccc cgcauuggaa uaacaaagag gcucuggugg      840

aguucaagga ugcccacgcu aagcggcaga caguuguggu ccucgggagc caggaaggag      900

ccgugcauac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu aaaggacgcc      960

ucuuuuccgg ccaccugaag uguagacuua aaauggauaa gcugcggcuc aaaggcguuu     1020

cuuacagccu gugcaccgcc guguguacag ccgcuaaggu ccccgcggag acgcuucacg     1080

ggaccgugac agucgaagug caauaugccg gaaccgacgg cccuugcaaa guucccgcuc     1140

agauggccgu ggauaugcag acacugaccc cugucgggcg ccucauuacg gccaaucccg     1200

ugaucacaga guccaccgaa aacagcaaga ugaugcugga gcuggacccg ccuuucggag     1260

auucuuacau uguuaucggc gugggggaca agaaaaucac ccaucacugg cauagauccg     1320

gcagcacaau uggaaaggcu uuugaagcca ccguccgggg cgcgaaacgc auggcugugc     1380

ucggggauac agccugggac uucggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6877
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 2 opt

<400>  6877
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auuagaugca      120

ucggcguuuc uaaccgggac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugcg cucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagcagau guccgacaca gggcgaagcc uaccucgaca      360

agcaguccga uacccaguau gucugcaaac ggacacuggu ggaccgcggg uggggaaacg      420

gcuguggccu uuuugggaag ggaagccugg ugaccugcgc uaaauucacg uguucuaaga      480

agaugaccgg caaauccauc caacccgaga aucucgagua cagaauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgaugaaaa ucgggccaag guggagguga      600

caccuaacag cccccgcgcu gaagccaccc ugggcggguu uggaucucuc ggccuggacu      660

gcgagccuag aacaggccuu gauuucuccg accuguauua ccucaccaug aauaacaaac      720

auuggcuggu ccacaaggag ugguuucaug auauuccccu uccuuggcac agcggggcgg      780

acacggaaac accgcacugg aauaacaaag aggcccuggu ggaguucaag gacgcucaug      840

ccaagcggca gaccguugug guccucggau cccaggaagg cgcugugcac acagcccugg      900

cgggggcccu ggaagcugag auggauggag ccaaaggccg ccucucuagc gggcaucuga      960

aguguagacu uaaaauggac aagcugcggc ucaaaggagu uuccuacagc cugugcaccg     1020

cugccuuuac cuucacaaag gugcccgccg agacgcuuca cggcaccguc acaguggaag     1080

uccaauaugc uggccgcgau gggccuugua aagugcccgc gcagauggcc guugacaugc     1140

agacccugac accuguggga agacucauca ccgcuaaucc cgucauuacg gagucuacag     1200

aaaacuccaa gaugaugcug gagcuggauc cgccuuuugg cgacagcuac aucgugauug     1260

ggguuggaga uaagaaaauc acccaccauu ggcaccgguc uggcagcauc auugggaagg     1320

ccuucgaagc caccgugcgc ggcgcuaaaa gaauggccgu ccucggagac acagcguggg     1380

auuuuggcuc cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  6878
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 2 opt

<400>  6878
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaggugau cuaccugguc augauuguuc ucaucgugcc cgccuauucc auuucuugca      120

ucggcgugag caacagagac cuggucgagg ggauguccgg aggcaccugg guggauguug      180

ugcuugaaca cgggggaugu gucacagaga uggcucaaga caaaccuacg guggauauug      240

aacugguuac caugacagug ucuaauaugg ccgagguccg gagcuacugc uaugaagcgu      300

cccucucuga cauggcuagc gccucccgcu guccgaccca gggcgagccc agccuggaua      360

agcagucuga cacacagucc gugugcaaaa gaacccuugg ggaucgggga uggggcaacg      420

gcugugggau cuucggaaag ggcagccugg ugacaugcuc uaaauuuacc uguugcaaga      480

agaugccugg gaaauccauc caacccgaga aucucgaaua ccgcauuaug cugccugucc      540

auggaagcca gcacucuggc augaucguua acgacauugg gcaugagacg gaugaaaaua      600

gagccaaggu ggaggugacc cccaacuccc cucgggcuga ggccacacug ggaggcuucg      660

gcagccucgg gcuggacugu gaaccgcgca ccggacuuga uuuuuccgac cuguauuacc      720

ucacaaugaa uaacaaacac uggcuggucc auaaggagug guuccacgau aucccccuuc      780

cuuggcacgc uggcgccgac accgggacgc cccauuggaa uaacaaagaa gcgcuggugg      840

aguuuaagga cgcccacgcu aagagacaga caguuguggu ccucggaucu caggaaggcg      900

ccgugcauac cgcucuggcc ggggcgcugg aggccgagau ggauggagcu aaaggccgcc      960

ucuucagcgg ccaccugaag ugccggcuua aaauggacaa gcugagacuc aaagggguuu     1020

ccuauagccu guguacagcc gcuuuuaccu ucaccaaggu gccugccgaa acacuucacg     1080

gaacggucac cguggagguc caaucugccg gcacagaugg gcccugcaaa gugccggcuc     1140

agauggcggu ugacaugcag acccugacac cugugggacg gcucauuacc gccaaucccg     1200

ucaucacgga auccacagag aacagcaaga ugaugcugga acuggauccu cccuuuggcg     1260

acucuuacau ugugaucggg guuggcgaua agaaaaucac ccaucacugg caucgcagcg     1320

gauccaccau uggcaaggcu uucgaggcca cagugagagg ggccaaacgg auggcugucc     1380

ucggagacac cgccugggau uuuggcucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6879
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 2 opt

<400>  6879
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

agaaggugau cuaccugguc augauucucc ugaucgcccc cgcuuauagc auucggugca      120

ucggcguuuc caaccgcgac uucguggagg ggaugucugg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caaaccuacg gucgauauug      240

agcuggugac cacaaccguu agcaauaugg cggaagugag auccuacugc uaugaggcuu      300

cuaucagcga cauggccucc gauucucggu guccgacaca gggcgaagcc uaccucgaca      360

agcagagcga uacccaguau gucugcaaac gcacacuggu ggacagaggg uggggaaacg      420

gcuguggccu uuuugggaag ggaucccugg ugaccugcgc uaaauucgcc uguagcaaga      480

agaugacggg caaaucuauc caacccgaga aucucgagua ccggauuaug cuguccgucc      540

augggagcca gcacucugga augaucguua acgauaccgg ccaugaaaca gacgagaauc      600

gcgcuaaggu ggaaauuacc ccuaacuccc ccagagccga ggcgacacuc gggggauuug      660

gcagccuggg ccuggauugc gagccucgga ccgggcuuga cuucucugau cuguauuacc      720

ucacgaugaa uaacaaacac uggcuggugc auaaggaaug guuucacgac aucccccuuc      780

cuuggcacgc cggagcugac acaggcaccc cgcauuggaa uaacaaagag gcccuggucg      840

aguucaagga ugcucacgcc aagcgccaga cagugguugu gcucgggucc caggaaggag      900

cgguccauac cgcccuggcu ggcgcccugg aagcugagau ggacggggcc aaaggaagac      960

ucagcuccgg ccaccugaag ugucggcuua aaauggauaa gcugcgccuc aaaggcgugu     1020

cuuacagccu gugcaccgcc gcuuuuacau ucacgaagau ucccgcggag acccuucacg     1080

ggacaguuac cguggaaguc caauaugccg gaacagacgg cccuuguaaa gugcccgcuc     1140

agauggccgu cgauaugcag acccugacgc cuguggggag acucaucaca gccaaucccg     1200

uuauuaccga guccaccgaa aacagcaaga ugaugcugga gcuggacccg ccuuuuggag     1260

auucuuacau cgugaucggc gucggggaaa agaaaauuac acaucacugg caucgguccg     1320

gcagcaccau cggaaaggcu uucgaggcca cagugcgcgg cgcgaaaaga auggcuguuc     1380

ucggggacac ggccugggau uuuggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6880
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 2 opt

<400>  6880
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agagagugau cuaccugguc augauucucc ugaucgcccc cgcuuauucc auucggugca      120

ucggcguuuc uaaccgcgac uucguggagg ggaugagcgg aggcaccugg guggaugucg      180

ugcuugaaca cgggggaugu guuacaguga uggcccaaga caagccuacg gucgauauug      240

agcuggugac cacaaccguu uccaauaugg cggaagugag aucuuacugc uaugaggcua      300

gcaucuccga cauggccucu gauagccggu guccgacaca gggcgaagcc uaccucgaca      360

aacaguccga uacccaguau gucugcaagc gcacacuggu ggacagaggg uggggaaacg      420

gcuguggccu uuuugggaaa ggaagccugg ugaccugcgc uaaguucacg uguucuaaaa      480

agaugaccgg caaguccauc caacccgaga aucucgagua ccggauuaug cugagcgucc      540

augggucuca gcacuccgga augaucguua acgauauugg ccaugaaaca gacgagaauc      600

gcgccaaagu ggaagugacc ccuaacagcc ccagagcuga ggccacacuc gggggauuug      660

gcucucuggg ccuggauugc gagccucgga ccgggcuuga cuucuccgau cuguauuacc      720

ucacgaugaa uaacaagcac uggcuggucc auaaagaaug guuucacgac aucccccuuc      780

cuuggcacgc gggagccgac acaggcaccc cgcauuggaa uaacaaggag gcucuggugg      840

aguucaaaga ugcccacgcu aagcgccaga caguuguggu ccucgggagc caggaaggag      900

ccgugcauac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu aagggaagac      960

ucuuuuccgg ccaccugaaa ugucggauua agauggauaa acuucgccug aagggcguuu     1020

cuuacagccu cugcaccgcc gccuucacau uuuccaaagu gcccgcugag acgcugcacg     1080

ggaccgucac aguggaaguc caauaugcgg gaagagacgg cccuuguaag gugcccgccc     1140

agauggcugu ugauaugcag acccuuacac cuguggggcg gcugaucacc gccaauccca     1200

uuaucacgga gagcacagaa aacucucgca ugaugcucga gcuggacccg ccuuucggag     1260

auuccuacau cgucauuggc gugggggaca aaaagaucac ccaucacugg cauagaagcg     1320

gcucuaccau uggaaaggcc uuugaagcua caguucgggg cgccaaaaga auggcggugc     1380

ugggggauac cgcuugggac uucggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6881
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  6881
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uaauaaaugc cagaaaggaa aaaaaaagaa gaggagcuga uacaagugua ggaauaguag      120

gauuauuauu gacaacagca auggcagcug aaguaacgag aagagguuca gcauauuaua      180

uguacuuaga caggaaugau gcaggugaag caauaucauu cccaacaaca cuaggaauga      240

auaaauguua uauacaaauu auggaccucg gacauaugug ugaugcuacg augucauaug      300

aaugcccuau gcuggacgag ggaguugaac cagaugaugu ugauuguugg ugcaacacaa      360

caaguacaug ggucguauau ggaacauguc aucauaaaaa aggagaagca cgaaggagua      420

gaagagcugu aacauuacca ucacauucaa cgcgaaagcu acaaacgcga ucacaaacau      480

gguuagaaag cagagaauac accaaacauc uaauuagagu agagaauugg auuuuuagaa      540

auccaggauu ugcacuggcg gccgcugcaa uugcauggcu auuaggaagu ucaaccaguc      600

aaaaaguaau auaccuagua augauauuac uaauagcgcc agcuuauagc auaagaugca      660

uaggaguuag uaaccgagau uuugucgagg gaaugucagg ugguacuugg guagacauag      720

ugcuagaaca ugguggaugu guaacaguua uggcacagga caaaccaaca guagacauug      780

aacucguaac aacaacugua aguaauaugg cagaaguacg gucauacugu uaugaagcua      840

guauaucaga uauggcaagc gacagcagau gcccaacuca aggggaagca uacuuagaua      900

aacaaaguga uacucaguau guauguaaac gaacauuagu agacagagga uggggaaacg      960

gauguggacu uuucgguaaa gguagccuag ugacaugugc aaaauuugcu uguaguaaaa     1020

aaaugacugg aaaaucaaua caacccgaaa aucuagaaua uagaauaaug uugucagucc     1080

acggaucaca gcauucuggu augauuguaa augauacugg acaugaaaca gaugaaaaua     1140

gagcaaaagu agaaauaaca ccaaacaguc caagggcaga agcaacauua ggugguuuug     1200

gaucacuagg guuagauugu gaaccaagga cgggauuaga uuuuagcgac uuauacuauu     1260

uaaccaugaa caauaaacac ugguuaguac acaaagaaug guuucacgau auaccguugc     1320

cguggcaugc gggagcugau acaggcacac cacauuggaa uaauaaagaa gcccuaguag     1380

aauucaaaga cgcacacgcg aaaagacaaa caguaguagu auuagguucg caagaaggcg     1440

cagugcauac cgcauuggca ggugcauugg aagcagaaau ggauggagca aaaggaaggu     1500

uaaguucugg acauuugaaa uguagauuga aaauggauaa acuaagacua aaaggaguga     1560

gcuauucauu auguaccgca gcauuuacau ucacaaaaau uccagcagaa acguuacacg     1620

gaacaguuac gguagaagua caauaugcag ggacagaugg ccccuguaaa guaccagcuc     1680

aaauggcggu ugauaugcaa acccuaaccc cagugggaag auuaauuaca gcuaacccag     1740

uaauaacaga aaguaccgaa aauagcaaaa ugaugcuuga acuagaccca ccauuuggag     1800

auucauacau aguaauagga guuggagaaa aaaagauaac acaucauugg cauaggagug     1860

gaaguacuau cggaaaagcg uuugaagcga caguuagagg agcaaaaaga auggcugugu     1920

uaggggauac agcaugggau uuuggaagcg ucggaggagc cuuaaauucu uuaggaaaag     1980

gaauucauca aauauuuggg gccgcauuca aaagucuauu ugguggaaug ucaugguuuu     2040

cacaaauauu aauaggaaca uuguuaaugu ggcuaggauu aaacacuaaa aacggaagca     2100

uaucacuaau gugucuagcu uuggguggag uacuaauauu ucuauccacu gcuguaucag     2160

cagacguggg guguagcgug gacuucagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6882
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 opt

<400>  6882
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaaugc gaggaaagaa aagaaaagaa gaggagccga cacuagcguc gguauaguag      120

guuuauuauu aacaacugcc auggccgccg aaguuacaag aagagguuca gcauacuaca      180

uguauuuaga uagaaaugau gcaggagaag cuauuuccuu uccuacaacu cuuggaauga      240

auaaauguua uauccaaauu auggaucuag gacauacgug cgaugcaaca augagcuacg      300

aauguccaau guuagaugaa ggaguagaac cagaugacgu ugauugcugg uguaacacaa      360

cuucaacaug gguaguuuau ggcacauguc aucauaagaa aggagaagca agaagaagua      420

gacgugcagu aacauuacca agucauucaa cuagaaaacu acaaacuagg agccaaacau      480

ggcuugaaag uagagaguau acaaaacacc uuauuagagu agaaaauugg auauuuagga      540

auccaggauu cgcguuagca gcagcugcaa uagcaugguu gcuaggaagc ucaacaaguc      600

aaaaaguaau auauuuagua augauacuuc uaauagcacc agcauauuca auaagaugua      660

ucggaguauc aaaccgagau uuugucgaag gaaugucugg ugguacaugg guagacguag      720

uauuagagca cggaggaugu guaacaguaa uggcacaaga uaaaccaaca guggauauag      780

aauuggucac aacaaccgua ucaaauaugg cugaagucag aagcuacugc uaugaagcau      840

cgauaaguga uauggcgagu gacucuagau gcccaaccca aggagaagca uacuuagaua      900

aacaaucaga cacacaauac guauguaaaa gaacuuuagu agauagaggu uggggcaacg      960

gauguggacu cuuuggaaaa ggaucgcuag uuacaugugc aaaauuugcc uguucaaaga     1020

aaaugacggg aaaaucaaua cagccagaaa auuuagaaua uagaauaaug uugaguguac     1080

auggauccca acauucagga augauaguaa augauacagg acaugaaacu gacgaaaaua     1140

gagcaaaagu ugaaauaaca ccaaauucac cacgugcaga ggcgacacuc ggugguuucg     1200

ggucauuggg ucuugauugu gaaccaagaa caggauuaga uuuuucagau uuauauuacu     1260

uaacaaugaa caauaaacac ugguuaguac auaaagaaug guuucaugau auaccguuac     1320

cauggcaugc aggagcagau acaggcacac cacauuggaa uaauaaagaa gcauuaguug     1380

aguucaaaga ugcgcacgcu aaaagacaaa caguugucgu auuagguagu caagaaggag     1440

caguacauac agcgcuagcu ggagcacuag aagcugaaau ggauggagca aaaggaagac     1500

uaaguagugg ucauuuaaaa uguagauuaa aaauggacaa acuuagguua aaaggaguua     1560

guuacucauu auguacugcg gcuuuuacau uuacaaaaau accugcagaa acauuacacg     1620

guacaguuac agucgaagua caauaugcug guacagacgg accuuguaaa guaccagcuc     1680

aaauggcagu agauaugcaa acacuaacac caguuggacg ccuaauaacc gcaaauccug     1740

uuauaacaga aucuacggaa aacucaaaaa ugauguuaga auuagauccc ccauuugggg     1800

auucauauau aguaauuggg guaggagaaa agaaaauaac acaucacugg cacagaagug     1860

gaaguacaau aggaaaagca uucgaagcaa caguaagagg agcaaaaaga auggcagucc     1920

uaggggacac ugcaugggau uucggaagcg uaggaggggc uuuaaauagu cucgggaaag     1980

gaauccauca aaucuuugga gcagcguuua aaucacuauu uggaggaaug ucaugguuuu     2040

cacaaauauu aauagguacu uuacuaaugu gguuaggacu aaaugcuaaa aacggcucaa     2100

uuucauuaau guguuuagca uuaggaggag uuuugauauu cuuaucaaca gcaguuagcg     2160

cugacguugg auguagugua gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6883
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opt

<400>  6883
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaacgc gagaaaagaa cguaaacguc gaggagcaga cacaagcaua ggcauaauag      120

gauuacugcu aacaaccgca auggcagccg aaauaacaag aagaggauca gccuauuaua      180

uguaucuaga uagaagugau gcagguaaag ccauaucauu ugcaaccacg uuaggaguaa      240

auaaaugcca uguacaaaua auggaccuug gacauaugug cgaugcaaca augucuuaug      300

aauguccuau gcuagaugaa ggaguagaac cagaugacgu ugauuguugg uguaauacaa      360

cuuccacaug gguagucuac ggaacaugcc aucacaaaaa aggugaagcu aggaggucaa      420

gacgagccgu aacauuacca ucucauucaa caagaaaauu acaaacaaga ucacaaacau      480

ggcuagaauc aagggaauau acaaaacauc uuauaaaagu agaaaauugg auuuucagaa      540

auccugguuu ugcacuagua gcaguagcaa uagccuggcu cuuagguagc aguaccagcc      600

aaaaaguaau auaucucguc augauucuau uaauagcucc agccuacagc auaagaugua      660

uuggaguauc aaauagagau uuuguagaag guaugucugg aggaacaugg guugauguag      720

uuuuagaaca ugggggaugu guaacaguaa uggcacaaga uaaaccaaca guggacauug      780

aauuagucac cacaacugua ucuaauaugg cagagguacg aucauauugu uaugaagcaa      840

gcauaagcga uauggcgagu gauucaagau gccccacaca aggagaagcu uauuuagaua      900

aacaaaguga uacacaguau guaugcaaaa gaacccuagu ggauagaggu ugggguaaug      960

guugcggauu auuuggaaaa ggaagucuag uaacaugugc aaaauucacu uguucaaaaa     1020

aaaugacagg gaaaaguaua caaccagaaa auuuagaaua uagaauaaug uuaagcguac     1080

augguucaca acauucggga augauaggau augaaacaga ugaagauagg gccaaaguag     1140

aaguaacacc aaacagucca agagcagaag ccacacuugg ugguuucgga ucucucggau     1200

uagacuguga accucgaaca gggcuugacu uuucagaucu auacuauuua acaaugaaua     1260

auaagcauug gcuaguucac aaagaauggu uucacgauau uccgcuucca uggcaugcgg     1320

gagcagauac aggaacucca cauuggaaua auaaagaagc acuaguugaa uucaaagaug     1380

cacaugcaaa aagacaaaca gucguaguuu uaggaucaca agaaggagca guacauacag     1440

cacuagccgg agcuuuagaa gcagaaaugg auggugcaaa aggacgacua uuuagcggac     1500

accuaaaaug ucgucuaaaa auggauaaac uuagauuaaa aggaguaucg uacucguuau     1560

guacagcagc auuuacauuu acuaaagugc ccgcagaaac acuacaugga acagugacug     1620

uugaaguaca auaugcagga acagauggcc cauguaaaau uccuguucaa auggcaguug     1680

auaugcaaac auuaacucca guuggaagac uaauaacagc aaacccuguu auaacagaau     1740

caacugaaaa uaguaaaaug auguuggaac uggacccacc auucggagau aguuacauag     1800

uaauaggugu aggagauaag aaaauaacac aucauuggca cagaucaggu aguaccauug     1860

guaaagcauu cgaagcaaca guuagaggag caaaaagaau ggcaguucuc ggagauacag     1920

caugggauuu ugguagugug ggagguguuu uuaacaguuu agguaaagga auacaucaaa     1980

ucuuuggugc agcuuuuaaa agccuauuug gaggaauguc augguucucu caaauucuaa     2040

uagguacacu auuaguuugg cuaggacuua auacgaaaaa ugggucuauu aguuuaacuu     2100

guuuagcacu cggaggagua augauauucu uaaguacagc aguauccgcu gacgucgguu     2160

guucaguaga uuucucaaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6884
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 2 opt

<400>  6884
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aaggaaagaa aagaaaagaa gaggagcaga cacaagcgua gguauaguag      120

gguuauuauu aacaacugcu auggcugcug aaguaacaag gagggguagu gcauauuaua      180

uguauuuaga cagaaacgau gcaggggaag caauaaguuu uccuacaaca uuaggaauga      240

auaaauguua uauccaaauu auggacuuag gccauaugug ugacgcuaca augaguuacg      300

aauguccaau guuagaugaa gguguagaac cagacgaugu ggauuguugg uguaacacaa      360

caucaacaug gguaguauau gguacauguc aucauaagaa aggagaagca agaagaucaa      420

ggagagcugu aacucuaccu ucacacagca caagaaaauu acaaacuaga ucacagaccu      480

gguuagaauc uagagaauau acaaaacauu uaaucagagu agaaaauugg aucuucagaa      540

accccggguu ugcucucgca gcagcagcaa uagcaugguu acuuggaagu ucuacaucac      600

aaaaaguuau uuaccuagua augauucuac uaauagcacc agcuuauagc auaagaugca      660

uuggaguuag uaacagagac uuuguagaag gaaugucagg ugguacuugg gucgaugugg      720

uauuagaaca ugggggaugc guaacuguua uggcacaaga caaaccaaca guagauaucg      780

agcuaguaac aacaacggua agcaacaugg cagaagugag aaguuauugu uaugaagcaa      840

gcauaaguga uauggcaucc gauucacgau guccaacaca aggugaagca uaccuagaua      900

aacaaucaga uacacaauau guauguaaaa gaacauuagu ugauaggggu uggggaaaug      960

gauguggacu auucggaaaa gggucauuag ugacgugugc aaaauuugcc ugcaguaaga     1020

aaaugacugg gaaaagcauu caaccagaaa aucuagaaua uagaauaaug cuaaguguac     1080

auggaucuca acauaguggc augauaguaa acgauacagg acacgaaacu gacgaaaaca     1140

gagcuaaagu agaaaucacu cccaauagcc caagagcuga agcaacacua ggaggauuug     1200

gaucauuagg acuugacugu gaaccuagaa caggauugga cuuuagcgau cuauacuauc     1260

uaacaaugaa uaauaaacau uggcuaguac auaaagaaug guuucaugau auuccacuuc     1320

cauggcacgc cggcgcagac acagggaccc cucacuggaa caauaaagaa gcauuaguag     1380

aguucaaaga ugcucaugca aaaagacaaa caguaguugu auuagguucg caagaaggag     1440

ccguacauac ugcacuugca ggagcuuuag aagcagaaau ggauggagca aaaggacgau     1500

uaucuagugg acaucuaaaa uguagacuaa aaauggacaa auuaagauua aaaggaguaa     1560

gcuauucauu auguacagca gcauuuacau ucacaaaaau accugcugaa acccuacacg     1620

gcacaguaac aguagaaguu caauaugcag gaacugaugg accuugcaaa guacccgcac     1680

aaauggcagu ugauaugcaa accuuaaccc cuguuggcag acuaaucaca gcaaacccag     1740

uuauaacaga aucaacagaa aauagcaaaa ugaugcuaga acuggaucca ccuuucggug     1800

auucauauau aguaauagga guaggggaaa agaaaauuac ucaucacugg cauagaucag     1860

gaucaacaau cgguaaagca uucgaagcua caguacgagg ggcaaaaaga auggcaguac     1920

uaggagauac ugcaugggau uuugguagug uagguggagc acuaaauucc uugggaaaag     1980

gaauacacca aauauuugga gcugcauuua aaagucuauu uggaggaaug agcugguuua     2040

gccagauucu aauuggaaca uuacuuaugu ggcucggauu aaacacaaag aauggaagua     2100

uuucauuaau guguuuagcu cucgguggcg uauuaauauu uuuaaguaca gcaguuagcg     2160

cagacguugg auguucagua gauuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6885
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  6885
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggaau uguuggacua uugcuaacaa cugcaauggc ugcagaggua acucgaagag      120

gcagcgcaua cuacauguau uuggauagaa augacgcagg ggaagcaaua agcuuuccaa      180

cgacauuagg aaugaauaaa uguuauauac aaauaaugga uuuaggacac augugugaug      240

caacuaugag uuaugagugc ccaaugcuug augaaggagu cgaaccagau gauguugauu      300

guugguguaa uaccacaagu acauggguug ucuacggaac augucaccau aaaaaaggug      360

aagcuagaag aaguagaaga gcaguaacac uacccaguca cucaacuaga aaacuacaaa      420

ccagauccca aacaugguua gaaaguaggg aauacacaaa acaucuaauu agaguugaaa      480

acuggauauu uagaaaucca gguuucgcuu uagcagcagc agcaauugcu ugguuauuag      540

gaaguucaac aucacagaaa guuauauacc uaguaaugau acuacuaauu gcacccgcau      600

auaguauacg auguauugga guuagcaauc gugauuucgu ugaaggaaug ucaggaggaa      660

cuuggguuga cauaguauua gaacauggag gguguguaac gguuauggca caagauaaac      720

caacgguuga uauagagcua guaacaacaa caguuagcaa uauggcagaa guacgaucau      780

auugcuauga agcuucaaua ucagauaugg caucagauuc gagaugucca acacaagggg      840

aagcauauuu agauaaacaa agugauacac aauauguaug uaaacgaaca cuaguagaua      900

gagguugggg gaauggaugu gguuuauucg gaaaaggauc acuuguaaca ugugcaaagu      960

uugcauguuc aaaaaaaaug acaggcaaaa gcauacaacc agaaaaucua gaauacagaa     1020

ucauguuauc aguacaugga ucucaacaua gcggaaugau aguuaaugac acaggacaug     1080

aaacagauga aaauagagcg aaaguagaga uaacaccuaa uaguccaaga gcagaagcaa     1140

cauugggagg auucggaucu cuaggacuag auugugaacc acgaacagga cuugacuuuu     1200

cugauuuaua cuaucuaaca augaacaaua aacacuggcu aguacauaaa gaaugguuuc     1260

acgauauacc acuaccaugg cacgccggag cagauaccgg aacaccacau uggaauaaua     1320

aagaagcauu aguagaauuu aaagaugccc augcuaaaag acaaacaguu guagugcuug     1380

gaagucaaga aggagcagua cauacagcau uggcuggugc auuagaagca gaaauggaug     1440

gugcaaaagg aaggcuaagu ucuggacauc uaaaauguag gcuaaaaaug gauaaauuaa     1500

gauuaaaagg aguaucauau ucauuaugua ccgcugccuu cacuuuuaca aaaauaccag     1560

cagaaacgcu gcacggaacu guaacaguug aaguacaaua ugcuggaaca gauggaccau     1620

gcaaaguucc ugcacaaaug gcuguagaca ugcaaacacu gacuccuguu gggagacuaa     1680

uuacagcaaa ccccguaaua acagaaagua ccgaaaacuc uaaaaugaug uuagaauuag     1740

auccaccuuu cggggauagu uauauaguaa uaggaguagg ggaaaaaaaa auaacucauc     1800

acuggcauag gagcggcagu acuauaggua aagccuuuga agcaacuguc agaggugcaa     1860

aaagaauggc aguguuaggu gacacagcau gggauuucgg aucaguagga ggugcauuaa     1920

auaguuuagg caaaggcauu caucaaauau uuggggccgc auucaaaagu cuguucggag     1980

gaaugagcug guucagucag auauuaauag gaacacuauu aauguggcua ggucuaaaca     2040

caaaaaacgg gaguauuagu cucauguguc uugcauuagg aggaguauua auauuuuuaa     2100

guacagcagu cucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6886
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 2 opt

<400>  6886
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

caguagguau aguagguuua cuauuaacaa ccgcaauggc agcagaagug acaagaagag      120

gaucagcaua cuauauguau uuagauagaa augaugcagg agaggcaauu ucauucccaa      180

caaccuuagg aaugaacaaa uguuauauac aaauaaugga uuuaggacau acaugugacg      240

caacaaugag uuacgaaugu ccaaugcuag acgaaggugu agaaccagau gacguagacu      300

gcugguguaa uacaacauca acauggguug uuuacggaac cugucaucau aagaaaggug      360

aagcaagaag aaguagacga gccguaacuc uaccaucaca cucaaccaga aaacuccaaa      420

caagaaguca gacauggcuu gaaagccgag aauacacuaa acauuuaaua agaguugaaa      480

auuggauuuu uagaaaccca ggauucgcau uagcagcagc agcaauagcg ugguuacuag      540

guucaucaac gucacaaaaa guuauuuauu uagucaugau ucuguuaaua gcaccagcuu      600

acaguauaag auguaucgga guaucaaaua gggacuuugu ugaaggaaug uccggaggua      660

cuuggguaga uguaguauua gaacauggag gauguguaac aguaauggca caagacaaac      720

caaccguaga uauagagcua guaacuacua caguauccaa uauggcagaa guacguaguu      780

auuguuauga agccagcaua agugauaugg caucagauuc aagaugccca acacaaggug      840

aagcauauuu agauaaacaa agugacacac aauacguaug uaaaagaaca uuaguagaca      900

gaggaugggg aaaugguugu ggauuauucg ggaagggauc acuuguaacu ugugccaaau      960

ucgcaugcuc aaagaaaaug acagggaaau cgauacaacc agaaaauuua gaauacagaa     1020

uaaugcucag uguacaugga agccaacauu caggaaugau cguuaaugau acuggucaug     1080

aaacagacga aaacagagcc aaaguugaaa uaacaccuaa uucaccaaga gcugaagcaa     1140

cauuaggagg auucggauca uuaggucuag acugugaacc aagaacaggu cuagacuuua     1200

gcgauuuaua uuaccuaacu augaauaaua aacauuggcu aguucauaaa gaaugguuuc     1260

augauauacc acucccuugg caugcaggag cagacacagg aacaccacau uggaauaaua     1320

aagaagccuu aguagaauuu aaagaugcac augcuaaaag acagacaguu guuguacucg     1380

gaucucaaga aggagcagua cauacagcau uagcaggagc auuagaagca gaaauggaug     1440

gugcaaaagg uagauuaucu aguggucacc uuaaaugucg auuaaaaaug gauaaauuaa     1500

gauuaaaagg gguuucauau agucuuugca cugcugcguu uacauuuacu aaaauaccag     1560

cagaaacucu acacggcaca guaacuguag aaguacaaua ugcuggaacu gacggccccu     1620

guaaaguacc agcgcaaaug gcugucgaua ugcaaacccu aacacccgua ggaaggcuga     1680

uaacagcaaa uccuguaaua acagaaagua cggaaaauuc aaaaaugaug uuagaacuag     1740

auccaccauu cggcgauuca uacauaguaa uuggaguagg agaaaagaaa auaacccacc     1800

auuggcauag gagcggaagc acaauaggaa aagcauucga agcaacagua agaggugcaa     1860

aaagaauggc aguacuagga gauacagcuu gggacuuugg auccguuggu ggagcauuaa     1920

auucguuagg uaaaggaaua caccaaauuu ucggagcagc uuucaaaucc cuauuuggag     1980

gaaugucaug guuuucccaa aucuuaauag gaacacuauu aauguggcua ggccuaaaug     2040

cuaaaaaugg aucuauauca uuaaugugcu uagcacuagg cggcguucug auauuuuuaa     2100

gcacugcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6887
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 2 opt

<400>  6887
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guauaggaau cauuggauua cuacuaacaa cugcaauggc agcagaaaua acuagacgag      120

gaucagcaua uuacauguau uuagauagau ccgaugcugg caaagcaauu ucauuugcaa      180

caacacuagg aguaaacaaa ugucauguuc aaauaaugga ucuaggacau augugugaug      240

ccacaauguc guaugagugu ccaauguuag augaaggugu agaaccagac gauguagauu      300

gcugguguaa uacgacaucc acauggguag uauauggaac uugucaucac aagaaaggag      360

aggcaagacg cuccaggaga gcaguuacau uaccaaguca uaguacaaga aaauuacaaa      420

caagaagcca aacuuggcuu gaaucgagag aauauacaaa acauuuaauu aaaguagaaa      480

auuggauauu uagaaaccca ggauucgcau uaguggcagu cgcaauugcc uggcuauuag      540

ggagcaguac aucacaaaaa guaauauauc ucguaaugau acuauuaaua gcuccugcau      600

acagcauaag augcauagga guuucaaacc gagauuuugu agaagguaug agcggaggca      660

cauggguaga cguuguacua gaacauggag gaugugucac ugugauggca caagacaaac      720

caacaguaga uaucgaacug guaacaacaa caguaaguaa uauggcagaa guaagauccu      780

auugcuauga agcaucgaua ucagacaugg caagugauuc uagaugccca acucaaggag      840

aagcauauuu agauaaacaa agcgacacac aauauguaug caagaggacu cuaguagaua      900

gagguugggg uaauggaugu ggcuuauuug gaaaaggaag ucucguaaca ugcgcaaagu      960

ucacauguuc aaagaaaaug acuggaaaau cuauacaacc agaaaauuua gaguauagaa     1020

uaaugcucag cguacauggu ucacaacaca guggaaugau cggauacgaa acugacgaag     1080

accgagcaaa aguugaagua accccuaauu caccaagagc agaagcaaca uuaggagggu     1140

ucggaagccu cggauuagac ugcgaaccac gaacaggauu agauuuuuca gaucuauacu     1200

auuuaacaau gaacaacaaa cauugguuag uucauaaaga augguuucau gauauaccac     1260

uaccauggca ugcaggcgcu gauacaggca cuccacauug gaauaacaaa gaagcauuag     1320

uagaauuuaa agacgcacac gcaaaacgac aaacaguagu aguuuuagga agccaagaag     1380

gagcgguaca cacugcauua gccggugcau uagaagcaga aauggauggu gcaaaaggaa     1440

gacuauuuuc uggucauuua aaaugucgau uaaaaaugga uaaauuaaga cucaaaggag     1500

uaucuuacag uuuauguaca gcagcauuca cauucacaaa aguaccugca gaaacauuac     1560

auggaacggu uacaguagaa gugcaauaug cuggaacaga uggaccaugu aaaauaccag     1620

ugcaaauggc uguagauaug cagacuuuaa caccagucgg aagauuaaua accgcaaauc     1680

caguaauaac agaaaguacc gaaaauucaa aaaugauguu agaauuagau ccaccauuug     1740

gcgacaguua uauuguuaua ggaguaggcg auaagaaaau aacacaucac uggcauagaa     1800

gcggaucuac aauaggaaaa gccuuugaag caacaguaag aggagccaaa agaauggcag     1860

uacucgguga uacagcuugg gauuucggau cuguuggagg aguuuucaau ucauuaggca     1920

aaggaauaca ucaaauauuc ggagcugccu uuaaaucauu auuuggugga augucauggu     1980

uuucucaaau ucuaauaggu acauuacuug uauggcuagg acuaaacacu aaaaaugguu     2040

caauaucacu aacaugucua gcuuuaggcg gaguaaugau cuucuuaagc acagcuguau     2100

cagcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6888
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 2 opt

<400>  6888
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau agucggacuu uuguuaacga cagccauggc agcugaagua acaagaagag      120

gcucugcaua cuauauguau cuagaccgga acgacgccgg agaagcaaua ucauucccaa      180

caacauuagg caugaauaaa ugcuacauac aaauuaugga ucuuggacau augugcgaug      240

caacaauguc uuaugaaugu ccaauguugg augaaggagu ugaaccugac gauguagacu      300

gcuggugcaa cacaacauca acauggguug uauaugguac cugucaucau aaaaaaggcg      360

aagccagaag aucaagaagg gcaguaacac ugccuaguca uaguacuaga aaauuacaaa      420

cuagaucaca aacuugguua gaaucaaggg aauacacaaa acaccuaaua cgaguagaaa      480

auuggauauu ucgaaaucca ggguucgcac uagcagcagc ugcaauagca uggcuuuuag      540

gaaguucaac aagccaaaaa gucauauauc uaguuaugau ucuacuuaua gcaccugcau      600

acucaauacg augcauagga gucucaaaua gggauuuugu agaagguaug ucaggaggga      660

ccuggguaga cguaguacua gaacauggug gauguguaac agucauggca caagauaaac      720

caacaguaga cauagaauua guaacaacga cugucuccaa uauggccgaa guuagaucau      780

acugcuacga agcuucuaua ucagauaugg caagcgauuc aagauguccc acccaaggug      840

aagcauaucu agacaaacaa ucagauacac aauacguuug uaaaagaaca uuaguagaca      900

gagguugggg aaauggaugu ggauuauuug gaaaagguag uuuaguaaca ugcgcaaaau      960

uugcuugcag caaaaaaaug acaggaaaau ccauacaacc agaaaaccuu gaguauagaa     1020

uaaugcucag uguucaugga ucacaacauu cuggaaugau aguaaaugac acaggacacg     1080

aaacugacga gaaucgugcu aaaguagaaa uaaccccaaa uaguccuaga gcagaagcaa     1140

cauuaggcgg auuuggaagu uuaggauuag auugugaacc aagaacaggc uuggauuuua     1200

gugaucuaua uuacuuaaca augaauaaca aacauugguu gguccauaaa gaaugguucc     1260

augauauacc auuaccaugg cacgcuggag cagauacugg aacaccgcau uggaauaaua     1320

aagaagcacu uguugaauuc aaagacgcuc augcuaaacg acaaacagua guuguacucg     1380

gaagccaaga aggcgcaguc cacacagcuc uagcaggagc guuagaagcu gaaauggaug     1440

gcgcaaaggg aagauuaagc ucaggacacc uaaaaugcag acuuaaaaug gacaaacuaa     1500

gauuaaaagg aguuaguuau agccuaugua cagcagcauu cacauuuacu aaaauaccag     1560

cugaaacauu acauggaacu guuacaguag aaguacaaua ugcaggaaca gaugguccau     1620

guaaaguacc ugcacaaaug gcaguagaua ugcaaacauu aacaccuguc ggaagauuaa     1680

uuacagcaaa uccuguaaua acggaaagua ccgaaaauuc aaaaaugaug cuagaauuag     1740

acccaccuuu uggcgauagc uacauaguaa uuggaguagg agaaaaaaaa auaacacauc     1800

auuggcaccg aaguggaagc acaauaggaa aagcauuuga agcaacaguu agaggagcaa     1860

aaagaauggc aguauuaggg gauaccgccu gggauuucgg uucuguagga ggugcacuaa     1920

auagucuugg aaaaggcaua caccaaauuu uuggagcagc uuucaaauca cuguuuggug     1980

gaaugaguug guuuucacaa auucuaauag gaacauuauu aaugugguua ggauuaaaua     2040

caaaaaacgg aucaauaagc uuaauguguc uagccuuagg aggcguauua auauuccuau     2100

cgacagccgu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6889
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 opt

<400>  6889
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaaguaau auaucuaguc augauauuau uaauagcacc agcauauuca auaagaugua      120

uaggagucuc aaacagggau uucguagaag gaaugagugg agguacgugg guagauauag      180

uuuuagaaca uggaggaugu guaaccguua uggcacaaga caaaccaaca guagacauag      240

aguuagucac aacaacagua agcaauaugg cagaaguaag aucauacugu uaugaagcaa      300

guauaaguga uauggcaagu gacucaagau guccaacuca gggagaagca uaccuagaca      360

aacaaucaga cacacaauac guauguaaac gaacacuagu ggauagagga uggggaaaug      420

guuguggacu auuuggaaaa ggaagccugg uuacaugcgc uaaauuugcc ugcucaaaaa      480

aaaugacagg aaaaucaaua caaccagaaa aucuagaaua ucggauuaug uuaagcguac      540

auggaagcca acacucagga augauaguaa augauacagg acaugaaaca gaugaaaauc      600

gagcaaaagu ggaaauaacc ccaaacagcc caagagcaga agcuacacua ggaggauuug      660

gauccuuagg acuagauugu gaacccagaa caggacuaga cuuuucugau uuauacuauc      720

uuacaaugaa uaauaaacau ugguuaguac acaaagaaug guuccaugau auaccauuac      780

cauggcaugc aggagcagau acgggcacac cacacuggaa uaauaaagaa gcauuaguag      840

aauuuaaaga ugcacacgca aaaagacaaa caguaguagu auuaggauca caagaaggcg      900

caguucauac agcccuagca ggagcacuag aagcagaaau ggauggagca aaaggaaggc      960

uuagcagcgg ucauuuaaaa ugcagauuaa aaauggauaa auuaagacua aaaggaguaa     1020

guuauagucu auguacagcc gcauuuacau uuacaaaaau accagccgaa acuuuacaug     1080

gaacaguaac aguagaggua caauaugcag gaacggacgg accauguaaa guaccagcac     1140

aaauggcugu agacaugcaa acacuaaccc caguaggcag auuaauaaca gcuaauccag     1200

uuauaacaga aaguacagaa aauucuaaaa ugaugcuaga auuagaccca ccuuuuggag     1260

acucauauau aguaauaggu guaggagaaa aaaaaauaac acaucauugg cauagaucag     1320

gaaguacaau aggaaaagca uucgaagcaa ccguaagagg agccaaaaga auggcgguac     1380

uaggugauac agcaugggau uuuggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6890
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 opt

<400>  6890
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaaguuau auauuuaguu augauuuuac uuauagcucc ggcauacagu auaagaugua      120

uaggaguauc aaauagagau uucguagagg gaaugagugg aggaacaugg guagauguag      180

uauuagaaca uggagguugu guaacaguaa uggcacaaga caaaccaaca guagauauag      240

aauuaguuac aaccacagua ucaaauaugg cagaaguaag aagcuauugc uaugaagcaa      300

guauaaguga cauggcaagu gauagcagau guccaacaca aggagaagca uauuuagaua      360

aacaaaguga uacacaauac guuuguaaaa gaacauuagu agauagaggu uggggaaacg      420

gaugugguuu auuuggaaaa gggucuuuag uaacuugugc gaaauuugcu ugcaguaaaa      480

aaaugacagg aaaaucaaua caaccagaaa auuuagaaua uaggauaaug uuaucaguac      540

auggaucuca acauagcggu augauaguaa augacacagg acaugaaaca gaugaaaaua      600

gagcaaaagu agaaauaaca ccgaauucgc ccagagcaga agcaacacug ggaggauuug      660

gaagccuagg acuagauugu gaaccaagaa cgggacuaga uuuuucagau cuguauuauc      720

uuacaaugaa uaauaaacau uggcuagugc auaaagagug guuucaugau auaccacuac      780

cauggcaugc aggagcagac acagguacac cacauuggaa uaauaaagaa gcacuaguag      840

aauucaaaga cgcacaugca aaaagacaaa caguuguugu acuaggaagc caagaaggag      900

caguacauac agcauuagca ggggcauuag aagcagaaau ggacggagca aaagguagac      960

uaucgagcgg acaccuaaaa uguagauuaa aaauggauaa acuacgauua aaaggaguaa     1020

guuacaguuu augcacagcu gcauuuacau uuacaaaaau accggcagaa acauuacaug     1080

gaacgguuac aguagaagua caauacgcug gaacagaugg acccuguaaa gugccugcgc     1140

aaauggcagu ggacaugcaa acauugacac caguaggaag acuaauaaca gcaaauccag     1200

uaauaacaga aaguacggaa aauaguaaaa ugauguuaga acuagauccu ccauuuggag     1260

acucauauau aguaauagga guaggagaaa aaaaaaucac acaccacugg cacagaucag     1320

gaucgacaau aggaaaagcc uuugaagcaa caguaagagg agcaaaaaga auggcuguau     1380

uaggggacac agcaugggac uuuggaaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6891
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opt

<400>  6891
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau uuaucuaguu augauauuau uaauagcucc agcauacagu auaagaugua      120

uaggaguauc aaacagagac uuuguagaag gaaugucagg aggaacaugg guugacguag      180

uauuagaaca cgguggaugc guaacaguaa uggcacagga caaaccaaca guugauauag      240

aauuaguuac aacaacaguu ucaaauaugg cugaaguaag aucauauugu uaugaagcaa      300

guauaaguga cauggccagc gacaguagau gcccuacaca aggagaagca uaccuagaua      360

aacaaagcga uacacaauac gucugcaaaa gaacccuagu agacagaggu uggggcaacg      420

guuguggacu auuugguaaa gguagcuugg uaacaugugc aaaauuuaca uguucaaaga      480

aaaugacagg aaaaucaaua caacccgaaa aucuugaaua uagaauaaug cugaguguac      540

auggaaguca acauucagga augaucggau acgaaacaga cgaagauaga gcaaaaguag      600

aaguaacacc aaauucaccg agagcagaag caacacuagg aggauuuggc ucacuaggau      660

uagacuguga accaagaaca ggacuagauu ucagugaucu auacuaccua acaaugaaca      720

acaaacacug guuaguacau aaagaauggu uucaugacau accauuaccu uggcacgcag      780

gagcagauac aggaacacca cauuggaaua auaaagaagc auuaguggaa uuuaaagaug      840

cucaugcaaa aagacaaaca guaguaguac uaggaaguca agaaggcgcc guacacacag      900

cucuagcagg agcauuagaa gcagaaaugg auggagcaaa aggaagauua uuuaguggac      960

acuuaaaaug uagauuaaaa auggauaaac uaagauuaaa agguguauca uauaguuuau     1020

guacagcagc uuucacauuu acaaaaguac cagcagaaac auuacaugga acagucacag     1080

uagaaguaca auacgcagga acagauggac cauguaaaau accaguacaa auggcaguag     1140

acaugcaaac acuaacucca guaggaaggu uaauaacagc aaauccagua auaacagaaa     1200

guacagaaaa uaguaaaaug auguuagaau uagauccccc auuuggagac aguuauauag     1260

ugauaggcgu aggagacaag aaaauaacac aucauuggca uagaucaggu ucaacuauag     1320

gaaaagcauu ugaagcuaca guaagaggug cgaaaagaau ggcaguacua ggagauacag     1380

caugggacuu uggaaguuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  6892
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 2 opt

<400>  6892
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaaguuau auauuuggua augauauuac uaauugcucc agcauauuca auacgaugca      120

uaggaguauc aaauagagau uucgucgaag gaaugagugg aggaacaugg guagauguag      180

uauuagaaca uggaggaugc guaacaguaa uggcacaaga uaaaccaaca guagauauag      240

aacuaguaac aacaaccgua ucaaacaugg cagaaguaag aucauauugu uaugaagcau      300

caauaagcga cauggcgucu gauaguagau gcccaacaca aggugaggca uauuuagaua      360

aacaaagcga uacacaauau guuugcaaaa gaacauuagu cgauagagga uggggaaaug      420

gauguggauu guucggaaaa ggaucacuag ucacaugugc aaaguuugca uguaguaaaa      480

aaaugacagg aaaaaguauc caaccagaaa acuuagaaua uagaauaaug cucaguguac      540

augguaguca acauaguggc augauaguga augauacagg ucaugaaaca gaugaaaauc      600

gagcgaaagu agaaauuaca cccaauucuc cuagagcaga agcaacuuua ggaggauuug      660

gaaguuuagg auuagauugc gaaccaagaa caggauuaga cuucagcgau cuauacuauu      720

uaacaaugaa caauaaacau uggcucguac auaaagaaug guuucaugac auaccucuac      780

cuuggcacgc aggagcagau acaggaacuc cacauuggaa uaauaaagaa gccuuaguag      840

aauuuaaaga ugcacaugca aaaagacaaa caguagucgu acuaggaagc caagaaggag      900

caguacauac ugcauuggca ggagcauuag aagcagaaau ggacggugca aaaggaaggu      960

uaucaagugg acaucuaaaa ugcagacuua aaauggauaa auuaagauua aaaggaguaa     1020

guuauucauu augcacagca gcauuuacau uuacaaaaau cccagcagaa acacuacaug     1080

gaacaguaac aguagaagua caauacgcag gaacugaugg accauguaaa guaccagcac     1140

aaauggccgu ugauaugcaa acauuaacac caguaggaag auuaauaaca gcaaauccag     1200

uaauaacaga aaguaccgaa aacaguaaaa ugauguuaga acuagauccu ccauuuggag     1260

acaguuacau aguuauagga guaggagaaa aaaaaauaac acaccauugg cacagaagug     1320

gaagcacaau agguaaagca uuugaagcaa caguaagggg agcaaaaaga auggcaguac     1380

uaggagauac agcaugggau uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  6893
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       2 opt

<400>  6893
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau uguuggauua uuacuaacaa cagcaauggc agcagaagua acaagacgag      120

gaagcgcaua uuauauguau cuggauagaa augaugcugg ugaagcaaua aguuuuccaa      180

caaccuuagg uaugaauaaa uguuacauuc aaaucaugga uuuaggacac augugugaug      240

caacaauguc auaugaaugu ccuaugcuag augaaggcgu ugaaccagac gauguggauu      300

guuggugcaa cacaacaagu acaugggugg uauaugguac gugucaucac aagaaaggag      360

aagcaagaag aucaaggaga gcaguuacac uuccaagcca uaguacaaga aaauuacaaa      420

caagaaguca aacaugguua gaaucaagag aguacacaaa acaccuaauu agaguagaaa      480

auuggauauu cagaaaucca ggguucgcac ucgcagcagc agcuauagca ugguuacuag      540

gaaguaguac aagucaaaaa guaauauauc uaguaaugau acuauugauc gcgccagcau      600

auucuauaag auguauagga guaagcaaua gagauuuugu agaagguaug ucuggaggua      660

cauggguaga uauaguauua gaacacggcg gaugcgucac aguaauggcc caagauaaac      720

caacaguaga uauagaacuc guaacaacaa caguuaguaa uauggcagaa guaagaagcu      780

acuguuacga agccaguaua ucagacaugg caagugauuc aagaugucca acacagggag      840

aagcauauuu agauaaacaa ucugacacac aauauguuug caaaagaacc uuaguagaca      900

gaggaugggg aaacggaugc ggacuaagcg gaaaagguag cuuaguaaca ugugcaaaau      960

ucgcaugcuc aaagaaaaug acuggaaaaa gcauacaacc agaaaacuua gaauauagaa     1020

uuauguuaag uguacaugga ucacaacaca guggaaugau aguaaacgac acuggacaug     1080

aaaccgauga aaaucgagca aaaguagaaa uaacaccuaa uagcccgaga gcggaagcca     1140

cacuaggagg auucggaagu uuaggacuag acugugaacc aagaacagga uuagacuuua     1200

gugaucuaua uuauuuaacc augaacaaua aacauugguu aguucauaaa gaaugguucc     1260

augacauacc auuaccaugg cacgcaggag cagauaccgg uacgccgcau uggaauaaca     1320

aagaagcucu aguagaauuu aaagaugcac augcaaaacg acaaacugua gugguacuag     1380

gaucacaaga aggagcaguu cauacagcuu uagcgggggc gcucgaagca gagauggacg     1440

gugcaaaagg uagauuaagu ucaggacacc uaaaauguag acuaaaaaug gauaaacuaa     1500

gauuaaaagg aguaucguau ucuuuaugua cagcugcguu uaccuuuaca aaaauuccag     1560

cagaaacauu acauggaacc gucacaguug aagugcaaua cgcaggaacu gaugguccau     1620

gcaaaguccc ugcacagaug gcaguagaua ugcaaacgcu aacaccggua ggacgauuga     1680

ucacugcgaa uccugugaua acugaaagca ccgaaaauuc aaaaaugaug cuggaauuag     1740

auccuccauu uggagacagu uacauaguaa uaggaguagg agaaaagaaa auaacacauc     1800

auuggcacag aucaggaagu acaauaggca aagcauuuga agcuacagug aggggagcaa     1860

aacgaauggc aguauuaggu gauacagcau gggacuuugg caguguagga ggggcacuaa     1920

auucuuuggg aaaagguaua caucaaaucu uuggagcagc auuuaaaagu uuauuuggag     1980

gaaugagcug guuuucacaa auuuugauag gcacuuuauu aaugugguua ggacuaaaua     2040

cuaaaaaugg aucaauauca uuaauguguu uagcuuuagg aggcguauua auauuuuuau     2100

ccacagcagu gagcgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6894
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 2
       opt

<400>  6894
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

ccguaggaau uguaggauua uuacuaacaa ccgcaauggc agcagaagua acgcgacgag      120

gaagugcaua uuacauguac uuagauagaa augacgcugg ugaagcaaua aguuuuccua      180

caacucuugg gaugaacaaa uguuacaucc aaauaaugga ucuuggccau acaugugaug      240

caacaauguc auaugaaugu ccaauguuag augaaggagu cgaacccgac gaugucgauu      300

guuggugcaa uaccacauca acauggguug uauauggaac augccaucau aagaaaggag      360

aagcgcggag aaguagacga gcaguaacau uaccaucaca uucgacacga aaauuacaaa      420

caagaaguca aacaugguua gaaagcagag aauacacaaa acauuuaaua agaguagaaa      480

acuggauauu uagaaaucca ggguuugcau uagcagcagc agcaauagca uggcuucuag      540

gauccaguac cucacaaaaa guaauauacu uaguaaugau auuauugauu gcaccagcuu      600

auucuauaag auguauugga guuagcaaua gagauuuugu agaagggaug aguggaggaa      660

cauggguaga uguaguuuua gaacacggag guuguguaac aguaauggca caagauaaac      720

caacaguaga uauagagcua guaacaacua cugugagcaa uauggcagaa guucgaucau      780

acuguuacga agcaucaaua agugacaugg caucggauag cagaugucca acacaaggug      840

aagcauaucu agauaaacaa ucagauacuc aauauguaug uaaacgaacu uuaguagaua      900

gaggaugggg aaauggaugc gguuuaagcg gaaaaggcuc auuaguuaca ugugcuaaau      960

ucgcuuguag uaagaaaaug acaggaaaau caauacaacc cgaaaaucua gaauaucgaa     1020

uaauguuauc cguacaugga agccaacauu cagguaugau aguaaaugac acaggacaug     1080

aaacagacga aaauagagcu aaaguagaaa uaacuccuaa uucaccaaga gcagaagcua     1140

cacuaggagg uuucgguagc cuaggguuag auugugaacc aagaacagga uuagacuuuu     1200

cagacuuaua uuaccuaaca augaauaaua aacauugguu aguucacaaa gaaugguuuc     1260

acgauauacc auugccuugg caugcaggag cagauacugg aacaccacac uggaauaaua     1320

aagaagcauu aguagaauuu aaagaugcac acgcaaaaag acagacaguu gucguauuag     1380

ggucacaaga aggagcugua cauacagcau uagccggagc cuuagaagca gaaauggacg     1440

gagcaaaagg aagacuaagu agcggacauc uuaaauguag acuaaaaaug gacaaacuac     1500

gacugaaagg aguaucauau ucauuaugua cagcagcuuu uacauucaca aaaauaccag     1560

cugaaacccu acauggaaca guaacagucg aaguucaaua cgcaggaacg gacggaccau     1620

guaaaguacc agcucaaaug gcaguagaca ugcagacuuu aacaccagua gggaggcuaa     1680

uuacagcaaa cccuguuaua acagaaucaa cggaaaacuc aaaaaugaug uuggaauuag     1740

auccaccuuu uggagacuca uacauaguaa uaggaguagg agaaaagaaa auaacacauc     1800

acuggcaucg aaguggauca acuauuggua aagcauuuga agcaaccgua agaggagcua     1860

aaagaauggc uguuuuaggu gauacagcgu gggauuuugg cucaguggga ggugcauuaa     1920

acucuuuagg caaagggaua caccaaauau uuggugccgc auuuaaauca uuauucggag     1980

gcaugucaug guucagccaa auauuaauag gaacauuauu aaugugguua ggucuaaacg     2040

caaagaacgg aaguauuucu cuaauguguu uagcauuagg aggaguucua auauuucuau     2100

caaccgcagu uucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6895
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 2 
       opt

<400>  6895
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caauuggaau aaucggcuua uugcuaacua cagcaauggc agcugaaauu acaagaagag      120

gaucagcuua uuauauguau cuagacagau cagaugcugg uaaagcaauu ucauucgcaa      180

caacacuagg cguaaauaaa ugucacguac aaauaaugga uuuaggccau augugcgaug      240

caacaauguc guaugaaugu ccaauguuag acgaaggagu ggaacccgau gauguagacu      300

guuggugcaa cacaacuuca acauggguag uauacggaac augccaucac aaaaaaggag      360

aagcaagaag aucaagacga gcaguuacuc uaccaagcca uaguacaagg aaacuacaaa      420

ccagaucaca aacauggcua gaaucacgug aauacacaaa gcauuuaaua aaaguagaaa      480

acuggauauu uagaaaucca ggguucgcau uaguggcagu agcaauagca uggcuauuag      540

gaucaucaac aucacaaaaa guaauauauu uaguaaugau auuauuaaua gcgccagcau      600

auaguauacg auguauagga guaucaaaca gagauuuugu ggaaggaaug ucaggaggua      660

caugggugga cguaguucua gaacacggag gcuguguaac aguaauggca caagacaaac      720

cuaccguaga uauagaacua gugaccacaa caguaucaaa cauggcggaa guaaggucuu      780

auugcuauga agccaguaua ucagauaugg caucugauag cagaugccca acacaaggag      840

aagcauaucu ugauaaacaa ucugauacac aauauguaug uaaaagaacc uuaguagauc      900

gaggaugggg aaaugguugu ggacuaucag guaaagguuc auuagucaca ugugcuaaau      960

ucacuuguag uaaaaaaaug acaggaaaau cuauacaacc agaaaaucuu gaauauagga     1020

uaauguuaag uguccacgga agucaacauu cagggaugau agguuaugaa acagaugaag     1080

acagggcaaa aguagaagua acaccaaaca gcccaagagc ggaagcaaca uuaggaggau     1140

ucggaagccu cggacuagac ugugaaccac ggacgggauu agacuucagc gaucuuuauu     1200

auuuaacaau gaauaauaaa cacuggcuag uucauaaaga augguuccau gauaucccuc     1260

uaccauggca ugcaggagca gauacaggaa caccccacug gaauaauaaa gaagccuuag     1320

uagaauuuaa agaugcccac gccaaaagac aaacgguagu uguauuagga ucacaagaag     1380

gagcaguaca uacggcguua gcuggcgccc uagaagcaga aauggauggu gcgaaaggaa     1440

gacuauuuuc aggccaucuc aaaugcagau ugaaaaugga uaaacuuaga cuaaaaggag     1500

uaucauauuc auuauguaca gcagcauuua cauuuaccaa aguaccagca gaaacauuac     1560

auggaacagu uacaguagaa guucaauaug cagguacaga uggaccuugu aagauaccag     1620

uacaaauggc gguagauaug caaacacuca cuccaguugg uagauuaauu acugcaaacc     1680

caguaauaac agaaucgacg gaaaauucua aaaugauguu agaauuagac ccaccauuug     1740

gagauaguua uauagugaua ggugugggag auaaaaaaau aacacaucau uggcaucgau     1800

cagggucaac uauagguaaa gcauucgaag caacagugag aggggcaaaa aggauggcag     1860

uacuaggaga uacugcaugg gauuuuggca gugucggugg gguuuuuaau agccuaggua     1920

aaggaauaca ccaaauauuu ggcgcagcgu ucaaaucauu auuuggcgga augucauggu     1980

uuucucaaau acuuauagga accuuacuag uaugguuggg uuuaaacacc aaaaauggaa     2040

guauuucacu aacauguuua gcccugggag gaguaaugau auuucuaagc acagcaguaa     2100

gugcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6896
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 2 
       opt

<400>  6896
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau aguaggacua cuauuaacaa cagcaauggc agcugaggua acaagaagag      120

gaagcgcuua uuauauguau uuagauagaa augaugcagg agaagcuauc agcuucccaa      180

caacacuagg aaugaacaaa uguuauauac aaauaaugga uuuaggccac augugcgaug      240

caacaaugag cuaugaaugu ccaauguuag augaaggagu agaaccugau gauguugauu      300

guuggugcaa caccacaagu acauggguag uuuauggaac augccaucac aagaaaggug      360

aagcacgaag aucaagacga gcaguaacac ugccaucuca uucaacacga aaauuacaga      420

caagaaguca gacauggcuu gaaaguagag aauauacgaa acaccuaaua cgcguagaaa      480

auuggauauu uagaaacccu ggauuugccu uggcggcagc agcaauagcu ugguuauuag      540

guucuaguac aucacaaaaa guaauauauc uaguaaugau acuacuaaua gcaccagcau      600

acucaauaag augcauagga guaagcaaca gagauuucgu agaaggaaug aguggaggaa      660

ccuggguaga uguaguccua gagcacggag gauguguaac aguaauggca caagacaaac      720

caacuguaga uauagaacuu guaacaacaa cgguaucaaa cauggcagaa guacgaaguu      780

acugcuacga agcaucaaua ucugacaugg cuucagauuc aagaugccca acacaaggag      840

aagccuaucu agauaaacaa ucagacacac aauacguuug caaacgaaca uuaguagacc      900

gagguugggg aaacggaugu gggcuaucag ggaaagguuc acuuguaaca ugugcaaaau      960

uugcaugcuc aaagaaaaug accgguaaaa guauucaacc agaaaaucua gaauacagaa     1020

uuaugcuauc aguacacgga ucacaacaua gugguaugau aguaaaugac acuggacaug     1080

agacugauga aaauagagca aaaguagaaa uaacaccaaa uaguccaaga gcagaagcaa     1140

cauuaggagg auuugguagu uuaggacucg acugugaacc acggacuggg cuggauuuua     1200

gugacuuaua uuacuuaacc augaauaaua aacacugguu aguacacaaa gaaugguucc     1260

augauauacc uuugccaugg caugcuggug cagauacagg aacuccccau uggaauaaua     1320

aagaagcacu uguagaauuu aaagaugcac acgcaaaacg acaaacagua guuguacuag     1380

guucccaaga aggagcagua cauacagcac uugcaggagc ucuagaagcu gaaauggaug     1440

gagcuaaagg acgauuaagu aguggacacc uaaaauguag acuuaaaaug gacaaauuaa     1500

gguuaaaagg aguaaguuau ucauuaugua cagcagcauu caccuucacu aaaauaccug     1560

cggaaacacu ccacgguaca guaacuguag aaguacaaua ugcaggaaca gauggaccau     1620

guaaaguacc agcacaaaug gcuguggaua ugcaaacacu aacucccgua ggaagacuaa     1680

uaacugcaaa cccaguaauc accgagagua cagaaaacuc uaaaaugaug uuagaauuag     1740

auccaccauu uggagauuca uacauuguaa uuggaguagg ugaaaagaaa auaacucacc     1800

auuggcauag aucuggaagu acaauaggaa aagccuucga agcaacagua agaggugcua     1860

aacggauggc aguauuagga gauacggcau gggauuuugg uucuguaggu ggagcacuaa     1920

acucccuagg gaaagggauu caccaaauuu uuggagcagc auuuaaauca uuauuuggag     1980

gcaugagcug guuuucacag auacuaauag guacauuacu uaugugguug gguuuaaaca     2040

cuaaaaacgg uucaauuuca uuaaugugcu uagcacuagg ugguguguua auauuucuaa     2100

guacagcggu uagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6897
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       2 opt

<400>  6897
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguaggaau uguagggcuc uuauuaacaa cagcuauggc agcagaagug acaagaaggg      120

guagugccua uuacauguac uuagauagaa augacgcagg ugaagcaauu ucauuuccaa      180

caacauuagg aaugaauaaa uguuacauac aaauaaugga ccuaggacau augugugacg      240

caacaaugag uuaugaaugu cccauguuag acgagggggu ugaaccagau gauguagauu      300

gcugguguaa uacaacgagu acguggguag uauauggaac gugucaucau aaaaaaggag      360

aagcucguag aaguaggaga gcaguaacuu uaccaaguca cucaacaaga aaauuacaaa      420

caagaagcca aacaugguua gaaucaagag aauauacaaa acaucucaua agaguagaaa      480

auuggauauu uagaaaucca ggauuugcau uagcagcugc ugcaauugcc ugguuacucg      540

gaaguuccac cucacaaaaa guaauauacc ucguaaugau cuuguuaaua gcaccagcuu      600

auucgauaag auguaucgga guuaguaaua gagauuuugu ugaaggcaug agcggaggga      660

cauggguaga uauaguacuu gaacauggcg gauguguuac aguaauggca caagauaaac      720

caacgguaga uauugaauua guaacaacaa cagucaguaa cauggcugaa guaagaucau      780

auugcuauga agcuaguaua ucugauaugg cuagugauag uagaugucca acucaaggag      840

aagccuauuu agauaaacaa agugacacuc aauauguuug uaaaagaaca uuaguagaca      900

gaggaugggg aaaugguugu ggauuauucg gaaaaggaag cuugguaaca ugugcaaaau      960

ucgcauguag uaaaaaaaug acaggaaaau cuauucaacc agaaaacuua gaauauagga     1020

uaauguuauc agugcacggc ucacaacaua guggaaugau aguacaagac acagggcaug     1080

aaacagauga aaauagagca aaaguagaaa uaacuccaaa caguccuaga gcugaagcaa     1140

cucuaggugg auuuggaagu uuaggcuuag auugugaacc aaggacagga uuagauuuua     1200

gugauuuaua uuaccuaaca augaauaaua aacauuggcu uguacauaaa gaaugguucc     1260

augauauucc acuaccaugg caugcaggag cagacacugg uacuccacau uggaauaaca     1320

aagaagcauu aguugaauuu aaagaugccc augcuaaaag acaaacagua guaguauuag     1380

gaagucaaga aggagcuguu cacacugcac uugcaggagc acuagaagca gaaauggaug     1440

gagcaaaagg acgguuaagc aguggacauu uaaaaugcag acuaaaaaug gauaaacuuc     1500

gauuaaaagg gguaucauau aguuuaugua cugcagcuuu uacguuuaca aaaauaccag     1560

cggaaacacu acauggaaca guuacaguug aaguacagua ugcaggcacu gauggaccuu     1620

guaagguacc agcgcaaaug gcaguagaca ugcaaacacu aacuccagug ggacgacuaa     1680

uaacagcaaa uccaguaaua acagaaagca cagaaaauag uaaaaugaug cuagaacuag     1740

acccaccauu uggagacuca uacauaguca uaggcguagg agaaaaaaaa auaacccauc     1800

auuggcacag aaguggauca acuauaggaa aagcauuuga agcaacaguu cguggagcaa     1860

agagaauggc aguauuggga gacaccgcau gggauuucgg uaguguugga ggugcacuua     1920

auucauuggg aaaaggaaua caucaaauuu uuggagcagc uuucaaaagu uuauucggcg     1980

ggaugucaug guucagucaa auauuaauag guacuuuacu aaugugguua ggauuaaaua     2040

caaaaaaugg uagcauuuca cuuauguguc uagcauuagg agguguuuug auauuucuau     2100

caacagcugu gucagcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6898
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 2
       opt

<400>  6898
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cagucggaau agucggacuu cuauuaacua cugcaauggc agcagaaguc acaagaagag      120

gaucagcuua uuacauguac uuagauagga augaugcugg agaagccauu agcuuuccca      180

ccacuuuagg caugaauaaa uguuacauac aaauaaugga uuuaggacau acaugugaug      240

caacaauguc auacgaaugu cccauguuag augaaggugu agaaccagau gauguugauu      300

guuggugcaa uacaacauca acauggguag uauacggaac augccaucau aagaaaggag      360

aagcaaggcg aucaagacga gcaguuacau ugccuaguca uaguaccaga aaauuacaaa      420

caagaucuca aacaugguua gaaaguagag aauacacaaa acaucuaaua agaguugaaa      480

acuggauauu cagaaaucca ggauuugcac uagcagcagc agccauagca uggcuauuag      540

gaucaagcac aagucaaaaa guuauauacu uagugaugau auuauuaaua gcaccagcuu      600

acucuauaag auguaucgga gucuccaaca gagauuuugu agaaggaaug ucaggaggaa      660

cauggguuga uguaguauua gaacauggug gauguguaac aguaauggca caagauaaac      720

caaccguaga uauagaauua gucacaacaa cuguaucaaa uauggccgaa guaagaaguu      780

acuguuauga agcaucaauu ucugacaugg cuucagauag ccgcugcccc acacaaggcg      840

aggcauauuu agacaaacaa agcgauacac aauauguaug uaaaagaacu cuaguugaua      900

gaggaugggg uaauggaugu ggacuauucg guaaagguuc auuaguaaca ugugcaaaau      960

uugcuuguag caagaaaaug acagggaaau caauccaacc agaaaaucua gaauauagaa     1020

uaauguuauc agugcacgga agucagcacu cagguaugau aguucaagau acaggacaug     1080

aaacagacga aaauagagca aaaguggaaa ucacgccaaa uucaccaaga gcugaagcua     1140

cccuaggugg cuuuggauca uuaggcuuag auugcgaacc aaggacagga uuagauuuuu     1200

cagaucucua cuauuuaaca augaauaaua aacacuggcu aguccacaaa gaaugguucc     1260

augauauccc auugccuugg caugcuggag cagauacagg uacaccacau uggaauaaca     1320

aagaagcacu cguagaguuc aaagaugcac augcaaaaag acaaacagua guaguacuag     1380

gaucccaaga aggugcaguu cacacugcac uggcaggagc cuuagaagca gaaauggaug     1440

gagcaaaagg aagauuauca aguggacacc uaaaauguag auuaaaaaug gauaaacuaa     1500

gauuaaaagg aguauccuau ucccucugca ccgcagccuu cacuuuuacu aaaauaccag     1560

cagaaacauu gcauggaaca guuaccguag aaguacaaua cgcaggaacu gauggacccu     1620

guaaaguacc cgcacaaaug gcaguagaua ugcaaacacu cacaccagua ggaagauuaa     1680

uaacagcaaa uccaguaaua accgaaucaa cagaaaacag caaaaugaug cuugaauuag     1740

auccaccuuu uggagauuca uauauaguaa uugguguagg cgaaaagaaa aucacacauc     1800

auuggcauag aaguggauca accauaggaa aagcauuuga agcaacagua agaggggcaa     1860

aacgaauggc gguccuagga gacacggcau gggauuuugg aucaguagga ggagcauuaa     1920

auucacuagg aaaaggcauu caucaaauau ucggagcugc auucaaaagu uuauucggag     1980

gaaugucaug guuuagucaa auacuuauag gcacauuauu aauguggcua ggauuaaacg     2040

ccaaaaaugg gucaauauca cucauguguu uagcuuuagg uggaguauua auauuuuuau     2100

ccacagcagu aucugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6899
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 2 
       opt

<400>  6899
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcgugggaau aguuggacua cuauuaacaa cagcaauggc agcagaagug acucgucggg      120

gaagugcaua uuauauguau uuagauagaa augacgcagg agaagcaauu aguuuuccaa      180

caacauuagg aaugaauaaa uguuacauac aaauuaugga ucuaggacac augugugaug      240

caacaauguc auaugaaugu ccgaugcuag acgaaggagu agagccagau gauguugacu      300

gcuggugcaa uacaacaagc acauggguag ucuacggaac uugccaucau aagaaaggag      360

aggcacgaag aagccggaga gcuguaacuu uaccuagcca uucuacucga aaauuacaaa      420

caagaucaca aacuuggcua gaaagucgag aauauacaaa acauuuaaua agaguagaaa      480

acuggauauu ccguaauccu ggguuugcuc uugcagcagc agcaauagcg uggcuacuag      540

gaaguagcac aagucaaaaa guaauauacu uagugaugau auuauuaaua gcuccagcau      600

auucaauuag auguaucggu gugaguaaua gagauuucgu agaaggaaug aguggaggga      660

cuuggguaga uguaguuuua gaacacggag gaugugucac agugauggcu caagacaaac      720

caacuguaga cauagaacuu guaacuacca caguaucaaa cauggcagaa guuagaaguu      780

auuguuauga agcaucuaua ucagauaugg caucagauuc acgaugucca acacaaggcg      840

aagcguaucu agauaaacaa ucggauacuc aauauguaug uaaaagaacu uuaguagaua      900

gaggaugggg aaauggaugu ggccuauucg gaaaaggauc auuagugaca ugcgcuaaau      960

uugccuguag caagaaaaug accgguaaaa guauucaacc agaaaauuua gaauauagaa     1020

uaauguuauc aguucacgga ucacaacaua guggaaugau aguucaagau acaggccacg     1080

aaacugacga aaauagagca aaaguagaaa uaacaccaaa uucuccaaga gcagaagcaa     1140

cacuaggcgg auuuggauca uuaggauugg auugugaacc ucgaacagga uuagacuuua     1200

gugauuugua uuauuuaaca augaacaaca aacauugguu aguacacaaa gaaugguuuc     1260

acgauauacc acuaccaugg caugcaggag cagauacggg aacaccccau uggaauaaua     1320

aagaagcgcu uguagaauuu aaagaugcuc augcaaaaag gcaaacagua guaguauuag     1380

gaagucaaga aggagcagua cauacagcuc uagcuggagc ccuggaagcu gaaauggaug     1440

gggcuaaagg aagacuuagc ucaggacauc uaaagugccg auuaaagaug gacaaauuaa     1500

gauuaaaagg cguaaguuau ucuuuaugua cagcagcauu uacauuuaca aaaauaccag     1560

cagaaacgcu gcaugguacu guaacaguag aaguacaaua ugcaggaacu gacggaccau     1620

guaaaguacc agcacaaaug gcgguugaua ugcaaacauu aacaccugua ggaagacuca     1680

uuacagcaaa cccaguuaua acagaaucua cagaaaauuc caagaugaug cuggaguuag     1740

aucccccauu uggugauagu uauauaguua ucgguguagg ugagaagaaa auaacacauc     1800

acuggcaccg aaguggauca acaauaggua aagcauucga agcaacagua agaggagcaa     1860

aaaggauggc aguauuaggu gacacagccu gggauuuugg aucuguagga ggagcacuua     1920

auucauuagg aaaaggcauu caccaaauau uuggagcagc auuuaaauca uuauucggug     1980

gaaugucaug guuuucacaa auuuugauag guacacuacu aauguggcua ggacuaaaca     2040

ccaaaaaugg gucaauaagu cuaaugugcu uagcguuagg aggaguacua auauuccuaa     2100

guacagcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6900
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6900
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

ccguagguau aguagguuua cuauuaacua cugcgauggc cgcagaaguc acaagaagag      120

guucagcuua cuauauguau cuagaucgua augacgcagg agaagcaaua ucauucccua      180

caacauuagg uaugaauaaa uguuauaucc aaauaaugga uuuagggcau augugugaug      240

caacaaugag uuaugaaugc ccaaugcuag augaaggagu agaaccagac gacguugacu      300

gcugguguaa uacaacaagc acauggguag uauacggaac augucaccau aaaaaaggag      360

aagcucgaag gucacgacga gcgguuacau ugccaucuca uaguacaaga aaacuacaga      420

caagaucaca gacauggcua gaaaguagag aauacacaaa acaucuaauu cgaguagaaa      480

acuggauauu uagaaaucca ggcuuugcac ucgcagcagc ugcaauagca uggcucuugg      540

gaaguucaac aucacagaaa guaauauacc uaguaaugau auuacuuaua gcaccagccu      600

auaguauuag guguauagga guaucuaaua gagauuuugu agaaggaaug agcggaggga      660

cauggguaga uauaguacua gaacauggag gauguguaac uguaauggca caagauaaac      720

caacaguuga cauagaacua guaacaacaa caguaucaaa uauggcagaa guaagaaguu      780

auuguuauga agcgaguaua agugauaugg caucagauuc aagaugcccu acacaaggag      840

aagcauaccu agacaaacaa ucggauacac aauauguaug uaaaaggaca uuaguugaua      900

gagguugggg gaacggaugu ggguuauucg gaaaaggaag uuuaguaacc ugcgccaaau      960

uugcauguag uaagaaaaug acagggaaaa guauccaacc ugaaaaucug gaauacagaa     1020

uuauguuaag ugugcaugga agucaacaca guggaaugau aguaaaugau acgggacacg     1080

aaacagauga aaauagggca aaagucgaaa uuacaccaaa cagccccaga gcagaagcaa     1140

ccuuaggagg auuuggauca uuaggauuag auugcgaacc aagaaccgga uuagacuuua     1200

gcgaucugua cuauuugaca augaauaaua aacauuggcu aguacauaaa gaaugguucc     1260

augauauacc auuaccaugg cacgcaggug cugauacugg cacaccacau uggaacaaca     1320

aagaagcacu cguugaauuc aaagacgccc augcaaaacg acaaacagua gucguuuuag     1380

gaucacaaga aggagccgua cacacugcau uagcaggagc auuagaagca gaaauggaug     1440

gcgccaaagg gagacuaucg aguggucauc uuaagugcag acuaaaaaug gauaaauuaa     1500

gguuaaaagg aguaaguuac ucucuaugca cagcagcauu uacauuuacu aaaauaccug     1560

cugagacauu acauggaacu guaacuguag aaguacaaua cgcagggacu gauggaccau     1620

guaaagugcc agcacaaaug gcaguagaua ugcaaacauu aacaccagua ggacgauuaa     1680

uaacggcaaa uccaguaauc acugaaucaa cugaaaauag uaaaaugaug uuagaguuag     1740

auccaccauu cggcgauagu uauauaguaa uaggaguggg cgaaaaaaaa aucacucauc     1800

auuggcacag aagcggcagu acauuaggaa aagcauucag cacaacauua aaaggagcuc     1860

aaagauuagc agcacuaggu gauacagcau gggauuucgg aucaauugga ggaguauuca     1920

auucaauagg gagagcagug caucaaguau ucgguggcgc auuuagaacu cuguucggag     1980

gaaugaguug gauaacacaa ggacuaaugg gugccuuauu auuauggaug ggaguaaaug     2040

cacgagaccg aucaauagca cuagcauuuc uagcaacugg agguguauua guauuucugg     2100

caacuaacgu ccacgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6901
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6901
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

guguaggaau aguagguuua cuccuuacca cagcaauggc ggcagaagua acacggagag      120

gaucugcaua uuacauguac cuugacagaa augacgcggg agaagcaaua aguuucccaa      180

caacacuagg gaugaauaaa ugcuacauuc aaauuaugga ucuaggacau acaugugaug      240

cgacgaugag cuaugaaugc ccaauguuag augaaggugu agaaccagau gauguagacu      300

gcugguguaa uacuacaucg accuggguug uauauggaac augucaucau aagaaaggag      360

aagcacgaag aagcaggaga gcaguaacau uacccaguca uucaacaaga aaauuacaaa      420

ccagaucaca aacauggcua gaaucaagag aauauacaaa acauuuaaua agaguagaaa      480

acuggauuuu uagaaaucca ggauuugcau uagcugcugc agcuauagca uggcuucuug      540

gcaguucaac cagccaaaaa guuauauauu uggucaugau uuugcuaaua gcaccagcau      600

auaguauacg auguauagga guaucaaaua gagacuuugu ugaaggaaug ucuggaggaa      660

caugggucga uguaguacua gaacauggag gauguguaac aguaauggca caagauaaac      720

caacaguuga uauagaauua guaacaacaa caguaucgaa uauggccgaa guaagaaguu      780

auuguuauga agcaucaaua uccgauaugg caucagauag cagaugucca acucaaggag      840

aagcauaccu agauaaacaa ucagauacuc aauauguuug uaaaagaaca uuaguugaca      900

gaggcugggg uaauggaugc gguuuauucg gcaaaggcuc auuaguaaca ugugcaaagu      960

uugcauguuc aaagaaaaug acaggaaaau caauacaacc agaaaauuua gaauaucgaa     1020

uaauguuaag uguacaugga ucccagcacu caggaaugau aguuaaugau acaggucacg     1080

aaaccgauga gaauagagca aaaguagaga uaacuccaaa uaguccaaga gcagaagcua     1140

cauuaggagg auuugguagc uuagguuuag auugugaacc acgaacagga uuagauuuca     1200

gugaucuaua uuaucuaacu augaauaaua aacacugguu aguacauaaa gaaugguuuc     1260

augauauccc acuuccaugg caugcaggag cagauacagg aacaccccau uggaauaaua     1320

aagaagcauu ggucgaauuu aaagaugcac augcaaaaag gcaaacaguu guaguguuag     1380

gaucacaaga aggagcugua cacacagcac uugcuggugc acuugaggca gaaauggaug     1440

gagcaaaagg aagacugagc agcgggcauu uaaaauguag auugaaaaug gauaaauuac     1500

gccugaaagg aguaaguuau agucuaugua ccgcagcauu uacauuuaca aaaauaccag     1560

cugaaacgcu acauggaacg gucacaguag aaguacaaua ugcagguaca gauggaccau     1620

guaaaguacc agcacaaaug gcaguagaca ugcaaacacu aacaccaguu ggaagauuga     1680

ucacugcaaa uccaguuaua acagaaucaa cugaaaauuc gaaaaugaug uuagaguuag     1740

acccuccguu uggugacuca uauauaguaa ucggaguagg agaaaagaaa auaacacauc     1800

acuggcauag aucaggaagu acacuaggaa aggcuuuuag cacaacauua aaaggugcuc     1860

aaagauuagc agcauuaggc gauacagcuu gggacuuugg aucaauaggc ggaguauuua     1920

acaguauagg aagggccgua caucaaguau uuggaggagc auuuagaaca cucuuuggag     1980

gaaugagcug gauaacacaa gguuuaaugg gagcauuauu acuuuggaug ggaguaaaug     2040

cacgagaucg aaguauagca uuagcauuuc uagcaacugg uggaguccua guauuucuag     2100

caacaaacgu acaugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6902
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 2 opt

<400>  6902
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caauagguau cauaggccua uuacuaacua cagcaauggc agcagaaaua acuagaagag      120

guucagcaua uuacauguau cuagauagaa gugaugcagg uaaagcaaua aguuuugcaa      180

caacauuagg aguaaauaaa ugccauguac aaauaaugga ucuaggucau augugugacg      240

caacaaugag uuaugaaugc ccuaugcuag acgaaggagu ugaaccugau gacguagauu      300

guuggugcaa cacaacaagu acuugggugg uauauggaac uugucaucac aagaaaggag      360

aagcaagacg aucaagacga gccguaacuc uaccaucuca cucaacaaga aaacuacaaa      420

cacgcucaca gacaugguua gaaucaagag aauacacuaa acauuuaauu aaaguugaaa      480

acuggauuuu ucgaaaucca gguuuugcau uaguagcugu agccauagca uggcuacuag      540

gaaguucuac aucucaaaaa guaauauauc uuguaaugau acuacuaaua gcuccagcau      600

auucaauuag auguauagga guaagcaaua gagauuucgu agaaggaaug aguggaggaa      660

cauggguaga uguaguauua gaacauggug guuguguaac aguaauggca caagacaaac      720

caacagucga cauagaauua guuacaacua cgguaaguaa cauggcagaa guuaggaguu      780

auuguuauga ggcaucaaua agcgauaugg cgucagauuc aagaugccca acacaaggag      840

aagcauauuu agacaaacaa agugauacgc aauacguaug uaaaagaacu cuaguagaca      900

gggguugggg aaauggaugc gguuuauuug gaaaaggcag ucuaguaaca ugcgcaaaau      960

uuacauguuc aaagaaaaug acaggaaaau cuauucaacc ugagaauuua gaauaucgaa     1020

uaauguuauc uguacaugga ucucaacauu caggaaugau aggguaugaa acugacgaag     1080

auagagcaaa aguagaagua acgcccaauu cuccaagagc agaagcaacc uuaggcggau     1140

uuggaaguuu aggacuagau ugugaaccaa gaacaggucu agacuuuuca gaucucuacu     1200

aucuaacaau gaauaauaaa cacuggcuug ugcauaagga augguuucau gauaucccau     1260

uaccauggca ugcaggagca gacacaggaa caccucauug gaauaauaaa gaggccuuag     1320

uagaauuuaa agaugcacau gcgaaaagac aaacaguagu aguuuuagga agucaagagg     1380

gagcaguuca uacagcauua gccggagcac uugaagcgga aauggaugga gccaaaggac     1440

gucuauuuag uggacacuua aaaugcagau uaaaaaugga uaaacuuaga cuaaaaggug     1500

ucucauacuc ucuaugcaca gccgcguuua cauuuaccaa gguaccagcc gaaacacuuc     1560

acggaaccgu aacuguagaa guacaauaug caggaacaga uggaccaugu aaaauuccug     1620

uacaaauggc aguagauaug caaacauuaa cacccguagg uagauuaaua acagcaaauc     1680

ccguaauuac agaaucaaca gaaaauagua aaaugauguu agaacuagau ccuccauuug     1740

gagauucaua uauaguaaua ggaguaggug acaagaaaau aacacaucau uggcacagaa     1800

gugguagcac auuaggaaaa gcauuuagua caacacucaa aggagcacaa agauuagcag     1860

cacuaggaga uacagcaugg gacuuuggaa gcauuggagg uguauuuaau aguaucggua     1920

gagcaguuca ucaaguguuu ggaggagcau ucagaacacu auuuggaggu augucuugga     1980

uuacacaagg uuuaaugggu gcauuauuau uguggauggg aguaaaugca cgagaucguu     2040

caauagcauu ggccuuuuua gcaaccggug gaguccuggu guuuuuagca acuaaugucc     2100

augcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  6903
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  6903
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cuguagguau aguaggacuc cuuuuaacaa cagcaauggc agcagaagua acgagaagag      120

guagcgcaua uuauauguau uuagauagaa acgacgccgg agaagcaauu agcuuuccua      180

caacauuagg caugaauaaa uguuauauac aaauaaugga ccuuggacau augugugaug      240

caacuaugag cuaugaaugc ccuaugcuag augagggagu agaaccagau gacguagauu      300

guugguguaa cacaacaagc acaugggucg uuuauggcac augccaucau aaaaaaggag      360

aggcaagacg uagucgaaga gcaguaacac uaccaucgca uucaacacga aaauuacaaa      420

cuagaagcca aacauggcua gaaucacgag aguacacaaa acauuuaaua agaguagaaa      480

acuggaucuu uaggaauccu gguuuugcac uagcagcagc agcaaucgca uggcuacucg      540

gaaguaguac gagucaaaaa guaaucuauc uaguaaugau uuuacuaaua gcacccgcuu      600

acucaauaag guguauaggu guaucaaaua gagauuuugu ggaagguaug agugguggaa      660

cauggguuga uguaguacua gaacauggag gaugugucac aguuauggcu caagauaaac      720

caaccguuga uauagaguua guuacuacua ccguuuccaa uauggcagaa guaagaagcu      780

acugcuauga agcaagcauc ucagauaugg caucagauuc aagaugccca acccagggag      840

aagcauauuu agacaaacaa ucagauacac aauauguaug caaaaggaca uugguugaua      900

gaggaugggg uaauggaugc ggauuauuug gcaaaggauc ucuaguaacc ugugcuaaau      960

ucgcaugcuc uaaaaaaaug acaggaaaau cgauucaacc ggaaaaucuu gaauaccgaa     1020

uaaugcuuuc aguacaugga agccaacauu caggaaugau cguaaacgau acaggucacg     1080

aaacagauga gaauagagcu aaaguagaaa uaacuccaaa uagcccacgu gcugaagcua     1140

ccuuaggagg auuugggucu uuaggauuag auugcgaacc acgaacagga uuagauuucu     1200

cagaccucua uuaucucaca augaacaaua aacauugguu aguccauaaa gaaugguucc     1260

augacauccc acuaccaugg cacgcaggug cggacacugg aacaccgcac uggaacaaca     1320

aagaagcacu agucgaauuc aaggaugcuc acgcaaaaag acaaacagua guaguacugg     1380

gaucacaaga aggagcagua cacacagcuu uagcaggagc auuagaagca gaaauggacg     1440

gagcaaaagg aagauuaagc agcggacauc uuaaauguag auuaaaaaug gauaaauuga     1500

gauuaaaagg agucucguau ucauuaugca cagccgcauu uacauuuaca aaaauacccg     1560

ccgaaacacu acauggaaca guuacugucg aaguacaaua ugcagggaca gacgguccau     1620

guaaaguacc ugcacaaaug gcaguugaca ugcaaacacu aacaccagua ggaagauuaa     1680

ucacagcaaa cccaguaaua acagaaucua cagaaaacag uaaaaugaug uuagaauuag     1740

auccaccuuu uggcgauucc uauauaguaa uuggaguagg agaaaaaaaa auuacacauc     1800

auuggcauag aagugguuca acacuaggca aagcuuuuuc aaccacacua aaaggugcuc     1860

agcgacuagc agcacuugga gauacagcau gggauuucgg aaguaucgga ggagucuuua     1920

acucaauagg ucgagcaguu caucaaguau ucggcggagc auuuagaaca cuauuuggug     1980

gaaugucaug gauaacacaa ggacuaaugg gagccuuauu auuauggaug ggaguaaaug     2040

cgagagauag aaguauugca uuagcauuuu uagcaacugg uggaguacua guauuuuuag     2100

caacuaacgu acaugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6904
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  6904
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaacgc ucgaaaagaa aaaaaaagac gaggagcaga uacaaguguu gguauaguag      120

guuuauuguu aacuacugca auggcagcag aaguaacaag acgaggaagc gcuuauuaua      180

uguaucuaga cagaaaugau gcaggagaag cgauaaguuu cccaacuaca uuagguauga      240

auaaauguua cauacaaaua auggauuuag ggcauaugug ugaugcaaca augaguuaug      300

aaugcccaau guuagaugag ggaguagaac ccgaugacgu agauugcugg ugcaacacua      360

caagcacaug gguaguauau gguacauguc aucauaaaaa aggcgaagca agaagaagcc      420

gaagagcagu uacacuacca ucacauucaa caagaaaacu ucaaacgcga ucacagacuu      480

ggcuagaauc aagagaauau acaaaacauc uaauuagggu agaaaauugg aucuucagaa      540

acccaggauu ugcgcuagca gcugcugcua uugcuugguu auuaggauca ucaacaagcc      600

aaaaaguaau uuaucuugug augauucuac uaauugcacc cgcauauucu auacgcugca      660

uaggaguguc gaaccgagau uuugucgaag gaauguccgg gggaaccugg guagauaucg      720

uauuagaaca uggagguugu guuacuguga uggcacaaga uaaaccaaca guagauauag      780

aauuaguuac aacaacagua agcaauaugg cagaaguuag auccuacugu uaugaagcau      840

ccauuagcga uauggcuagu gauagcagau guccaacaca aggagaagcu uacuuagaua      900

aacaaaguga cacacaauau guaugcaaaa ggacauuggu agaccgagga uggggcaaug      960

gaugcgguuu auucgguaaa ggcucauuag uaacuugugc aaaauuugcg uguucaaaaa     1020

aaaugacagg aaaaaguaua caaccagaaa auuuagaaua cagaauaaug uuaucaguac     1080

acggauccca acacucagga augauaguaa augacacagg acaugaaaca gaugaaaaca     1140

gagcaaaagu agagauaaca ccaaauucac caagagcuga agccacauua ggaggauucg     1200

gaaguuuagg uuuagacugu gaaccaagaa cuggauuaga uuuuagcgac uuauauuauu     1260

uaacaaugaa caauaaacau ugguuaguac auaaagaaug guuucaugau auaccauuac     1320

ccuggcaugc aggagcugau acagggacuc cccauuggaa caauaaagaa gcacuuguag     1380

aauucaagga ugcacaugcg aaaagacaaa caguaguagu cuuaggcagc caagaagggg     1440

caguucacac agcauuagca ggagcuuuag aagcggagau ggauggcgca aaaggaagau     1500

uaagcucagg ccacuuaaaa ugcagguuaa agauggacaa auuaagauua aaaggaguaa     1560

guuauagccu augcacagca gcauuuacau ucacuaaaau accagccgaa acauuacacg     1620

gaaccguuac uguugaagua caauacgcag gaacagacgg acccugcaaa guaccagcuc     1680

aaauggccgu agauaugcaa acauuaacuc ccguugggag acuaauaaca gccaauccag     1740

uaauaacaga aaguacugaa aauucaaaaa ugauguuaga auuagaucca ccuuuuggag     1800

acucauacau aguuauaggu guaggggaaa agaaaauaac acaccacugg cacagaucag     1860

gcucaacuau ugguaaagca uuugaagcua cagucagagg cgcgaaaaga auggcaguac     1920

uaggagauac agcaugggau uucggaucag uuggaggggc auuaaacagu uuaggaaaag     1980

gaauacauca aaucuucgga gcagcuuuca aaucuuuauu uggagguaug ucuugguuuu     2040

cucagauacu aauagguaca uuauuaaugu gguuaggacu aaauacaaaa aaugggucaa     2100

ucucuuugau guguuuagca cuagguggag uguugauauu uuuaucaaca gcaguaagug     2160

cagacguagg uuguucagua gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6905
<211>  2521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6905
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

uuaacgcuag gaaagaaaaa aaacgaagag gggcagauac auccguaggc auaguagguu      120

uguuauuaac aacagccaug gcagcagaag uaacacgaag aggcucagca uacuacaugu      180

auuuagauag aaaugaugca ggagaagcaa uaagcuuucc aacuacauua ggaaugaaua      240

aauguuauau acaaaucaug gaucuaggac acauguguga ugcgacaaug ucauaugaau      300

gcccaauguu ggaugaagga guugaaccag augaugucga uugcuggugu aauacaacaa      360

guacaugggu aguauaugga acaugccauc auaaaaaagg agaagccaga agaagcagac      420

gcgcaguaac acucccaucu cauaguacaa gaaaacuaca aacaagaucu caaaccuggc      480

uagagagcag agaauacaca aaacauuuaa uaagaguaga gaauuggauu uucagaaauc      540

cuggauuugc ccuggcagca gcagcuauug cauggcuacu ugguucgagc acgagccaaa      600

aaguaaucua ucucguuaug auacuacuaa uagcuccagc uuacagcaua agauguauug      660

gaguaucaaa uagagauuuu guagaaggua ugucaggagg aacaugggua gauaucguuu      720

uagaacaugg aggaugugua acaguaaugg cgcaagacaa accaacggua gacaucgaau      780

uaguaacaac gacaguaagu aauauggcag aaguaagaag uuacugcuac gaagcaagua      840

uuucagauau ggcaucagac ucacgauguc caacacaagg agaagcauau uuggauaaac      900

aaucagacac acaauaugua ugcaaaagaa cacuaguaga cagaggaugg gggaacggau      960

guggacuuuu uggaaaaggu ucuuuaguaa caugugcaaa auuugcgugc ucaaaaaaga     1020

ugacaggaaa aaguauacaa ccagaaaauc uugaauauag aauaauguua ucaguacacg     1080

ggagccagca uagcggcaug auaguuaacg acacuggaca cgaaacagac gaaaacagag     1140

cuaaaguaga aauaacccca aauaguccca gggcagaagc aacguuagga ggauuuggaa     1200

guuuaggacu agacugugaa ccacgaacag gacuagauuu uagcgaucua uacuacuuaa     1260

caaugaacaa caaacacugg cuaguccaua aagaaugguu ucaugacaua ccguuaccau     1320

ggcaugcagg agccgacacc ggaacuccac auuggaauaa caaagaggca uuaguagaau     1380

uuaaagaugc acaugcaaaa agacagacug uaguaguacu aggaagucaa gaaggagcug     1440

uacacacagc acuugcagga gcauuagaag cagaaaugga cggagcaaaa gggcgguugu     1500

cauccggaca ucuaaaaugu cgauuaaaaa uggacaaauu aaggcuuaaa ggaguaucuu     1560

acagucuaug cacagcagca uuuacauuua caaaaauacc agcagaaaca uuacauggua     1620

caguaacagu ugaaguacaa uaugcaggaa cagauggccc auguaaaguu ccagcacaga     1680

uggcaguaga uaugcaaacu uuaaccccag uaggaagauu gauaacagcu aaucccguaa     1740

uaacagaaag uacagaaaau ucaaaaauga uguuagaacu ggauccacca uuuggagaua     1800

gcuauauagu aauaggagua ggagagaaaa agauaacaca ucauuggcac agaaguggaa     1860

gcacaaucgg aaaagcuuuu gaagcaacgg uaaggggcgc aaaaagaaug gcaguauuag     1920

gagacaccgc gugggauuuu ggaagcguag gaggagcauu aaauaguuua ggaaaaggaa     1980

uacaucaaau cuucggagcu gcauucaaau cauuauuugg ugguauguca ugguuuucac     2040

aaauacuaau agguacccuu cuaauguggu uaggauuaaa uaccaaaaac ggcagcauaa     2100

gcuuaaugug uuuagcauug ggugguguuu uaauuuuuuu aucuacagca guuucagcag     2160

augucgguug uagcguggau uuuagcaagu gaggacuagu gcaucacauu uaaaagcauc     2220

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2280

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2340

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2460

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2520

u                                                                     2521


<210>  6906
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6906
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

augcaagaaa agaaaaaaaa cgaagaggag cagacacaag ugucggaaua guagguuuau      120

uauuaacuac cgcaauggcg gcagaaguaa cgagaagagg aucugcauau uacauguauu      180

uagaucguaa ugaugcugga gaagcaauau cuuucccaac aacacuagga augaauaaau      240

gcuauauuca aauaauggac cuaggacaua ugugcgaugc aacaaugagu uaugaauguc      300

caauguuaga cgaaggagua gaaccagacg auguggacug uugguguaau acaacuagca      360

cguggguagu cuacggaaca ugccaucaua aaaaagggga agcaagaaga aguagacgag      420

caguaacguu accaucacau agcaccagaa aauuacaaac caggucacaa acuuggcuag      480

aaucacgaga auacacaaaa caucuaauua gaguagaaaa cuggauauuu cgaaacccgg      540

gauuugccuu agcugcagca gcaauagcau ggcuauuagg aucaaguacu ucacaaaaag      600

uaauuuaucu aguaaugaua cuauuaauag cgccagcaua uucaauaagg uguauuggag      660

uuagcaauag agauuuugua gaaggaauga guggaggaac uugggucgau aucguacuag      720

aacauggagg auguguaaca guaauggcac aagacaaacc aacaguagau auagaacuag      780

uuacaacaac aguaaguaau auggccgaag ugagaagcua uuguuaugaa gccucaauau      840

cagauauggc aagugacuca agguguccaa cucaaggaga agcauaucug gauaagcaau      900

cagacacaca auaugucugu aaaagaacgu uaguagauag agguugggga aauggaugug      960

gucucuucgg aaaaggauca uuaguaacau gugcaaaauu ugccugcagc aaaaaaauga     1020

caggaaaaag cauacaaccu gaaaaccugg aauauagaau aaugcucuca guacauggua     1080

gccaacauuc agguaugaua gugaaugaua caggacauga aacugaugaa aaucgagcaa     1140

aaguagaaau cacgccuaau aguccgagag cugaagcuac acuaggagga uuuggaaguu     1200

uaggauuaga cugugaacca agaacaggau uagauuuuag ugaccucuac uauuuaacaa     1260

ugaauaauaa acauuggcuc guacauaaag agugguuuca cgacauacca uuaccauggc     1320

acgcaggugc cgauacagga acuccgcauu ggaauaacaa agaagcauug guugaauuua     1380

aagaugcaca ugcaaagaga caaacaguag uuguuuuagg aagccaagaa ggagcagucc     1440

acacagcuuu agcaggagcu uuagaggcag aaauggaugg agcaaaagga agacuuagcu     1500

cuggccaccu aaaaugcaga uugaaaaugg auaaacugag acuaaaagga guaucuuaua     1560

gucuauguac agccgcauuc acauuuacua aaauuccagc agaaacuuua caugguacag     1620

uaacuguaga aguucaauau gcaggaacag auggcccuug uaaaguaccu gcccaaaugg     1680

ccguagauau gcaaacauua acaccaguag gaagauuaau aacagcaaau ccaguaauaa     1740

cagaaagcac cgaaaauagc aaaaugaugu uagaauuaga uccaccauuu ggugauaguu     1800

auauaguaau uggaguaggc gaaaaaaaaa uaacacauca cuggcauaga ucagggucaa     1860

caauagguaa agcauuugaa gccacaguaa gaggagcaaa aagaauggca guuuuaggag     1920

auaccgccug ggacuuuggu ucaguaggag gagcucuuaa uucuuuagga aaaggaauac     1980

accaaauauu uggcgcagca uuuaagaguu uauucggagg aaugucaugg uuuucacaaa     2040

uucuaauagg aacguuauua auguggcuag guuuaaauac aaaaaauggg agcaucuccc     2100

uaaugugucu ugcauuagga ggaguacuua uauuuuuaag cacugcagua agugcggacg     2160

uagguuguag uguagauuuu agcaaaugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  6907
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6907
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cuagaaaaga aaaaaaacgu agaggugccg acacaagcgu aggaauugua ggguuauuau      120

uaacaacagc aauggcagcu gaaguaacua gaagagguuc agcauacuau auguacuuag      180

auagaaauga cgcuggagaa gcaauaaguu uuccaacaac auuagguaug aauaaauguu      240

auauacaaau aauggaucuu ggucauaugu gcgaugccac aaugucguac gaauguccaa      300

ugcuagauga aggagucgaa ccagaugaug uagauuguug guguaacaca acaagcacuu      360

ggguagugua ugguacaugu caucacaaaa aaggagaggc aagaagaagu agacgggcug      420

uuacacuucc cagccacucu acgagaaaac uacaaacacg aagucaaaca uggcuagaau      480

caagagaaua cacaaaacac cuaauaagag uagaaaauug gauauuuaga aauccagggu      540

ucgcucuugc agcagcagca auagcauggc uauuaggaag uaguacuagu caaaaaguga      600

uauaucuagu aaugauacuu uuaauagcgc cagccuacag cauaagaugu aucggaguaa      660

guaauagaga uuuugucgaa ggaaugucug gagguacuug gguagacaua guacuagaac      720

acgguggaug cgugacugua auggcacagg acaaacccac aguagacauu gaauuaguaa      780

caacaacagu aucaaacaug gcagaaguaa gaucauauug uuaugaagca aguauaagug      840

auauggcauc agauagucga uguccaacac aaggagaagc uuauuuagau aaacaaagug      900

auacgcaaua cguauguaaa agaacgcuag uagacagggg uuggggaaac ggaugugguu      960

uauucgguaa ggguucauua guaacaugug ccaaauuugc auguaguaaa aaaaugacug     1020

gaaaaucuau acaaccugaa aauuuagaau acagaauuau guuaucgguu cauggaagcc     1080

agcacagcgg aaugauaguu aaugauacag gacaugaaac ggaugaaaau agagcaaaag     1140

uagaaauaac accaaacagu ccaagagcag aagcaacauu aggaggauuu ggaagcuuag     1200

gauuggauug ugagccgaga acaggauuag auuuuagcga ucuauacuau uuaacaauga     1260

auaacaaaca uugguuagua cauaaagaau gguuucacga uauuccacua ccguggcaug     1320

caggagcaga uacagguaca ccgcauugga auaacaaaga ggccuuaguu gaguucaaag     1380

augcucaugc uaaaagacaa acggucguag uacuaggauc acaagaagga gcaguacaca     1440

cagcacuagc aggagcucuu gaagccgaaa uggauggugc gaaaggacga uuaucaagug     1500

gacauuuaaa augcagacua aaaauggaua aauugagauu aaaaggagua aguuacagcu     1560

uauguacagc ugccuuuacu uucacaaaaa ucccagcuga aacauuacau ggaacaguua     1620

caguugaagu ucaauaugca ggaacagaug guccauguaa aguaccagca caaauggcag     1680

uagauaugca aacauuaacu ccaguaggaa gauuaauaac ugccaauccu guaaucacag     1740

aaaguaccga aaauucaaaa augauguuag aauuggaucc gccauuugga gauucuuaua     1800

uagucauagg uguaggugaa aaaaaaauaa cacaucauug gcauagaagu gguucaacaa     1860

uagguaaagc auucgaagca acaguaaggg gagcaaaaag aauggcagua cuaggagaua     1920

cagcuuggga cuuugguagc gucggaggug cuuuaaauag cuuaggaaaa gguauucauc     1980

aaauauuugg ugccgcauuu aaaaguuuau ucggagggau gaguugguuu agucaaauac     2040

ucauuggaac ucuuuugaug uggcuaggau uaaacacaaa aaauggaagu auuucacuaa     2100

uguguuuagc auuaggagga guauuaauau uccuaucuac agcagucuca gcugauguag     2160

gaugcucagu cgacuuuagu aaaugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6908
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6908
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

gaaaagaaaa gaaaaggaga ggagcagaca caaguguagg aauaguaggu uuauuacuga      120

cuacggcaau ggcagcagaa guuacaagaa ggggaagcgc auauuauaug uauuuagaua      180

gaaaugaugc aggagaagca auaucauucc caaccacauu aggaaugaac aaauguuaca      240

uacaaauaau ggaccucgga cauaugugcg augccacaau gucauaugaa ugcccgaugu      300

uagacgaagg aguagaacca gaugauguag auuguuggug uaauacuacu agcacauggg      360

uaguauacgg aacaugccau cauaagaaag gagaagcucg aagaucuaga agggcuguaa      420

cuuuacccuc acauucaaca cguaaauuac agacaagaag ccaaacaugg cuagaaucaa      480

gggaauauac aaaacacuua auuagaguag aaaauuggau auucagaaau ccaggauucg      540

cguuagcagc cgcagcgaua gcuugguuau uaggaucaag uacaucucaa aaaguaauau      600

aucuagucau gauacuacua auagcgccag cuuacaguau aagaugcaua ggaguaagua      660

acagagacuu uguagaagga augucaggag gcacaugggu agacauagua cuagaacaug      720

guggaugugu aacagugaug gcacaagaca aaccaacagu agauauagaa uuaguaacaa      780

cgacaguaag caauauggca gaaguaagga guuauuguua ugaagcauca auuucagaua      840

uggcaagcga uucaagaugu cccacucaag gugaagcaua ucuagauaaa caaagcgaua      900

cgcaauaugu auguaaaaga acuuuaguag aucgaggcug ggguaacgga ugcgguuuau      960

uuggaaaagg gagcuuaguc acaugugcaa aauucgcaug uucgaagaaa augacuggaa     1020

aaaguauaca accugaaaau cuagaauaca gaauuauguu aucaguacau ggaagucaac     1080

auaguggaau gauuguaaac gauacagguc augaaacgga cgaaaaucgc gcaaaaguag     1140

aaauaacacc uaauagucca cgagcagaag cuacuuuagg ggguuucggg ucauuaggau     1200

uggacuguga accaagaaca gguuuagacu uuagugaccu auauuacuua acaaugaaca     1260

auaaacacug guugguacac aaagaauggu uucaugacau accauuaccc uggcaugcag     1320

gagcagacac uggaacucca cauuggaaua auaaagaagc gcuaguagaa uuuaaagaug     1380

cacaugcaaa aagacaaacu guaguugucc uaggaucaca agaaggagca guacauacag     1440

cauuagcugg agcauuagag gcagaaaugg auggagcaaa aggaagauua uccagugggc     1500

aucuaaaaug cagguuaaaa auggauaaac uuaggcuaaa aggaguaucu uacucacuau     1560

guaccgcugc auucacauuu acaaaaauuc cugcagaaac auuacaugga acagugacag     1620

uagaagugca auaugcugga acugacgguc cauguaaagu uccagcacaa auggcaguag     1680

auaugcaaac auuaacuccu guagguagac uuauaacagc aaacccagua auaacagaaa     1740

guacagaaaa uucaaaaaug augcuagaau uagauccacc auucggagau agcuauauag     1800

ugauuggagu aggugaaaag aaaauuacac accacuggca caggaguggu aguacaauag     1860

gaaaagcguu ugaagcaacu guaagaggug caaaaagaau ggcugucuua ggugacacug     1920

caugggauuu ugguaguguu ggaggagcuu uaaauucacu aggaaaaggu auacaccaaa     1980

uauucggagc agcauuuaaa agucuauuug gcgguauguc cugguuuuca caaauauuaa     2040

uaggaacguu auuaaugugg uuaggacuaa acacgaaaaa uggaagcaua ucauugaugu     2100

guuuagcucu aggaggaguu uuaauauuuc uuucaacagc agucucagca gauguuggau     2160

guucaguaga cuuuaguaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6909
<211>  2509
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6909
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aagaaaaaaa aagacgagga gcagacacau cagucggaau uguaggacua cuuuuaacaa      120

cagcaauggc agcagagguu acuaggagag guucagccua uuauauguau uuagauagaa      180

acgaugcugg cgaagcaauu aguuucccca caacauuagg aaugaauaaa uguuauauac      240

aaauuaugga uuugggucau augugcgacg caacaaugag uuaugaaugc cccaugcugg      300

augaaggggu cgaaccagac gauguagauu guugguguaa cacaacauca accuggguag      360

uauacgggac augucaucac aaaaaaggag aagcgagacg aagcagaaga gcaguuacac      420

uaccaaguca cucaacacga aaacuacaaa cacgaucaca aacaugguua gaaaguagag      480

aguacacaaa acaucuuauu cgaguagaga auuggauauu uaggaaucca ggauuugcau      540

uagcagcagc agcaauagca ugguuauuag guucaucaac aagucaaaaa guaauauacc      600

uuguaaugau acuacuuaua gcaccugcuu auaguauaag auguauugga guaucaaaua      660

gagacuuugu cgaaggaaug aguggaggca cguggguaga uauaguauua gaacacggag      720

gauguguaac agucauggca caagacaaac cuacaguaga caucgaacuc gucacuacca      780

caguaucaaa uauggcagaa guaagaucau acuguuauga agccaguaua agugauaugg      840

caagugacuc aagaugucca acacaaggug aagcauaucu cgauaaacaa uccgauacuc      900

aauauguaug uaaaagaaca uuaguagaua gaggaugggg caauggaugu ggacuuuuug      960

gaaaaggguc acuaguaaca ugcgcaaaau uugcauguag uaaaaaaaug acagguaaau     1020

caauacaacc agaaaaccuu gaauaucgaa uaauguuaag uguucaugga agccaacacu     1080

cgggcaugau cgugaacgac acagggcacg aaaccgauga gaauagagca aagguagaaa     1140

uaacaccaaa uagcccaaga gcagaagcua ccuuaggagg auucggauca uuaggcuuag     1200

auugugaacc aagaacaggg uuagacuuuu cagaucuaua cuaucuaacc augaauaaua     1260

aacauuggcu aguucacaaa gaaugguuuc augauauacc auuaccaugg cacgcaggag     1320

cagacacggg aacaccacau uggaacaaca aagaagcauu aguggaauuu aaagaugcac     1380

acgcaaaaag acaaacuguu gugguacuag gaagucagga aggagcagua cauacagcau     1440

uagcgggagc gcuagaagca gaaauggaug gagcaaaagg aagacuauca ucuggucacu     1500

uaaaauguag auugaaaaug gauaaguuaa gauuaaaggg gguuagcuau ucucuaugua     1560

cagcggccuu cacuuuuaca aaaauuccgg cagaaacacu gcauggaaca guaacaguag     1620

aaguccagua ugcagguaca gacggaccau guaaaguacc cgcccaaaug gcaguagaua     1680

ugcaaacauu gacuccagua ggaagacuca uaaccgcuaa uccgguuaua acagagagua     1740

cagaaaacuc aaaaaugaug cuagaacucg auccgccauu cggagauucg uauauaguua     1800

uagguguugg agaaaaaaaa auaacacacc auuggcauag aucaggcucc acaauaggaa     1860

aagccuuuga agcuaccgua aggggagcaa aaagaauggc aguauuagga gauacugcau     1920

gggauuucgg aucaguugga ggagcacuaa auagcuuagg aaaaggaaua caucaaauau     1980

uuggagcagc auuuaaaucu uuguuuggag gaaugucaug guucagucaa auauuaauag     2040

gaacguuauu aaugugguua ggucugaaua ccaaaaaugg aagcauuagu uuaauguguu     2100

uagcauuagg aggaguauua auauuucuau cgacagcugu aucagcagau guggguuguu     2160

caguagauuu uuccaaauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2220

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2280

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2340

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2460

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2509


<210>  6910
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6910
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaag       60

aaaaaaaaag aagaggagca gauacaucag uaggaaucgu aggauuacua uugacaacag      120

cuauggcagc agaaguaaca agaagaggau cggcauauua uauguauuua gaucgcaacg      180

augcuggaga agcaauuuca uucccaacca cuuugggaau gaauaaaugc uacauacaaa      240

uaauggauuu gggacauaug ugugacgcga cuaugucaua cgaguguccg augcuagaug      300

aaggaguaga accugaugac guagauuguu gguguaauac uacaagcacu uggguagucu      360

augguacgug ccaucauaaa aaaggugaag cucgaagaag cagaagagca guuacuuuac      420

cuucacacuc aacaagaaaa uuacaaacga gaagucaaac augguuagaa ucaagagaau      480

auacaaaaca uuuaauaaga guugaaaauu ggauauuuag aaauccuggu uuugcauuag      540

cugcggcagc aauagccugg uuauuaggau caucaacuag ucaaaaagua auuuauuuag      600

uaaugauuuu auuaauagca ccugcguaua guauaagaug uauaggagua aguaauagag      660

auuuuguaga agguauguca ggaggaacau ggguagacau aguauuggaa cauggggggu      720

gcguaacagu cauggcucaa gacaaaccaa caguagacau agaauuaguu acaacaacug      780

uauccaauau ggcagaagua agaagcuauu guuacgaagc uucuauaagc gacauggcuu      840

cagauucaag auguccuaca caaggugaag cauauuuaga caaacaaagu gauacccaau      900

acguaugcaa aagaacauug guggauagag gaugggguaa uggaugcgga uuauuuggaa      960

agggaucacu aguaacaugu gcaaaauuug cauguagcaa aaaaaugaca ggaaaaagua     1020

uacaaccgga aaaucuagaa uauagaauaa uguuaucggu acacgguagu caacauucag     1080

gaaugauagu aaaugauaca ggacaugaaa cagaugagaa cagagcuaaa guugaaauua     1140

caccgaauuc uccaagagca gaagcaacgc uaggaggauu uggaagucua gguuuagauu     1200

gcgaaccaag aacagguuua gacuucagug acuuauacua cuuaacaaug aacaauaaac     1260

auugguuagu acacaaagaa ugguuucaug auauaccacu accauggcau gcaggagcgg     1320

auacuggaac accacacugg aacaauaaag aagcauuagu agaauuuaaa gaugcacaug     1380

caaaaagaca aacaguagua gugcucgguu cacaagaagg agcagugcac acagcauugg     1440

ccggagcacu agaagcagaa auggauggag caaaaggaag acuaucauca ggacaucuaa     1500

aaugcaggcu aaaaauggau aagcuuagau uaaaaggagu aucauauuca cugugcacag     1560

cagcuuucac auucaccaaa auuccugcag aaaccuugca cgguaccgua acaguagaag     1620

uacaguaugc cgggacagau ggaccaugua aaguaccagc gcaaauggcg gucgauaugc     1680

agacucuaac accaguggga agguuaauaa cagcuaaucc cgugauaaca gaaucaacag     1740

aaaauaguaa aaugauguua gaacuagauc cuccauuugg agauucauac auaguaauag     1800

gaguuggaga aaaaaaaaua acacaucacu ggcauaggag cggcucaacc auuggaaaag     1860

cauucgaagc uacaguaaga ggcgcaaaac guauggcagu auuaggagau acagcauggg     1920

auuuuggauc aguuggaggu gcauuaaacu cacucggaaa aggaauacau caaauauucg     1980

gagcugcuuu caaauccuug uucggaggua ugaguugguu uucucaaaua uuaauaggaa     2040

cacuauugau gugguuagga uuaaauacaa aaaacggaag cauaucauua auguguuuag     2100

cauuaggggg ugucuuaauu uucuuaucaa cagcaguguc agcagauguc ggguguagcg     2160

uugauuuuag uaaaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6911
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6911
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaaa       60

agaaaagaag aggagcagau acaucagugg gaauuguagg gcuauuauua acaaccgcaa      120

uggcagcaga aguaacaaga aggggaagcg cauacuauau guauuuagac cguaaugacg      180

cuggagaagc gauuuccuuu cccacaacau uaggaaugaa uaaaugcuac auacaaauaa      240

uggacuuagg acauaugugu gaugccacaa ugucauacga auguccaaug cuugacgaag      300

gaguagaacc agaugacguc gacuguuggu gcaauacaac aucuacuugg guuguuuaug      360

ggacuuguca ucacaagaaa ggagaggcca gacgaagucg uagagcagua acuuuaccaa      420

gccacucaac aagaaaacua cagacuagaa gucaaacgug guuagaauca agagaauaua      480

caaaacauuu aauacgagug gaaaacugga uauucagaaa cccuggauuc gcauuggcag      540

cagcugcaau ugcaugguua uuaggaucaa guacaucaca aaaaguaaua uauuuaguua      600

ugauauuacu aauugcaccc gcuuauucaa uaagauguau aggaguaucu aacagagauu      660

uuguugaagg caugagcgga ggaacauggg uagauauagu auuggaacau gggggaugcg      720

uuacaguaau ggcacaagau aaaccaacag uggauauaga auuagucaca acaacaguua      780

guaacauggc agagguacga ucuuauuguu acgaagcaag uauaagugau auggcaucgg      840

auaguagaug uccuacacaa ggagaggcuu auuuagacaa acaaagugau acacaauaug      900

uuuguaaaag aacacuuguu gauagagguu gggguaacgg uugcggauua uuugguaaag      960

gcucacuggu uacgugugca aaauucgcau guagcaagaa aaugacugga aaaaguauac     1020

aaccagaaaa ucuagaauau agaauaaugu uaaguguaca cggaagccaa cauucaggaa     1080

ugauaguaaa ugacacagga cacgaaacag acgaaaauag agcaaaaguu gaaaucacuc     1140

cuaauucacc aagagcagaa gcaacacuag gagguuuugg aucucuaggc cucgacugug     1200

aaccacgcac aggauuagac uuuucagacu uauacuaucu uacaaugaau aacaaacauu     1260

ggcuaguaca uaaagaaugg uuccacgaca uuccacuacc auggcacgca ggagcagaca     1320

caggaacacc acauuggaac aacaaagaag cacuuguaga auuuaaagau gcccaugcca     1380

aaagacaaac aguaguagua uuaggaucac aagaaggcgc uguacauacu gcccuagccg     1440

gcgcacuaga agccgaaaug gauggcgcaa aaggaagacu aucaaguggg cauuuaaaau     1500

guagacuuaa aauggauaaa cugcgacuaa aaggaguauc guacucauua uguacggcag     1560

cuuuuacauu uaccaaaaua ccagcagaaa cacuacacgg aacuguaacg guggaaguuc     1620

aauacgcugg cacagaugga ccuuguaaag uaccagcaca aauggcagua gacaugcaaa     1680

cuuuaacacc aguaggaagg cuaauaacag ccaacccggu aauaacugaa aguacagaaa     1740

auucaaaaau gaugcuagaa uuagauccuc cguucggaga uagcuacaua guaauuggug     1800

uaggagaaaa gaaaauaacc caucauuggc accgaagugg aucuacaauc ggaaaagcau     1860

ucgaagcaac aguaagagga gccaaaagaa uggcuguacu uggggauaca gcaugggauu     1920

uuggaucagu agggggagcg cucaauaguu uaggaaaagg aauacaucaa auauuuggag     1980

cagcuuuuaa aaguuuauuu ggaggaauga guugguucuc acaaauauua auagguaccc     2040

uacuaaugug guuaggacuu aauacuaaaa auggaaguau aucauugaug uguuuagcau     2100

uagguggagu ucuaauauuu uuaucaacag caguaagugc agauguaggu ugcucaguag     2160

acuucagcaa augaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  6912
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6912
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

aaaggagagg agcggauaca ucaguaggaa uuguaggauu acuguuaacu acugcaaugg      120

cagcagaagu uacaagaaga ggaagugccu auuauaugua uuuggacaga aacgacgcag      180

gagaagcaau aucauuucca acaacauuag gaaugaauaa auguuauaua caaauaaugg      240

auuuaggaca uaugugugau gccacaaugu cuuaugaaug uccuauguua gacgaaggag      300

uagaaccuga ugacguugau uguuggugca acacaacaag uacuugggua guauauggaa      360

caugccauca caaaaaaggg gaagcaagga gaucaagaag ggcaguuaca uuaccaucuc      420

acucuacgag aaaauuacag acaagaucac aaacgugguu agaaaguaga gaauauacaa      480

aacacuugau acgagucgaa aauuggaucu uccggaaucc agguuuugcg cuagcagcag      540

cagcaauagc auggcuauua ggaaguucaa caagucaaaa aguaauauac uuagugauga      600

uuuuguuaau ugcaccugcg uacaguauaa gauguauagg agugucuaau agagacuuug      660

uagaaggaau gucaggcgga acauggguag auauuguacu agaacacgga ggaugcguga      720

caguaauggc acaagacaaa cccacaguag auauagaauu aguaaccaca acaguaagca      780

auauggcaga aguaagaagc uauuguuaug aggcaaguau aucagauaug gcaagugauu      840

caagaugucc aacucaggga gaagcuuauu uagauaaaca aagcgacaca caauauguau      900

guaaaagaac acuuguagac agaggauggg gaaacgggug cgguuuauuu ggaaaagguu      960

cauuaguaac cugugcaaaa uuugcaugcu cuaaaaaaau gacuggaaaa aguauacaac     1020

ccgaaaauuu agaauauaga auaaugcuaa guguacacgg aucacaacau ucagggauga     1080

uaguaaauga uacgggacau gaaacagaug aaaauagagc aaaaguagaa auaacuccca     1140

acaguccaag agcagaagcg acacuaggug gguuugguag uuuagggcug gacugcgaac     1200

caagaacggg uuuagacuuu ucagauuugu auuaucuaac uaugaacaau aaacauuggu     1260

uaguacauaa agagugguuu cacgacauuc cgcuuccaug gcaugccggu gcagacacag     1320

gaacaccaca uuggaacaau aaagaagcau uaguagaauu uaaagaugca caugcaaaaa     1380

gacagaccgu aguagucuua ggaagucaag aaggagcggu acacacugca cuagcaggag     1440

cacuagaagc agaaauggac ggagcaaaag gaagacuguc aaguggacac cuaaaaugua     1500

gguuaaaaau ggauaaacua agauuaaaag guguuucaua uucacuaugu acagcagcau     1560

uuacauuuac gaaaauacca gcagaaacau uacacgguac aguaacagua gaaguacagu     1620

augcaggaac agauggaccu ugcaaaguac cugcacagau ggcuguagau augcaaacac     1680

uuacaccagu aggcagacua auaacagcaa auccaguaau uaccgaaagu accgaaaauu     1740

caaaaaugau guuagaacuu gauccaccuu uuggagacag cuauauagua auaggggugg     1800

gagaaaaaaa aauaacacac cauuggcaua gaaguggaag uaccauaggg aaagcauuug     1860

aagcaacagu uaggggagca aaaagaaugg cgguauuagg ggacacagcu ugggacuuug     1920

gaaguguugg uggagcauua aauucauuag gaaaaggaau acaucaaaua uuuggagcag     1980

cauuuaaauc auuauuugga gggaugaguu gguuuaguca aauauuaaua ggaacacuau     2040

uaaugugguu aggguuaaau acuaaaaaug gcaguauaag cuuaaugugu uuagcacuag     2100

ggggagugcu aauauuucua aguacagccg uaagugcaga cgucggaugc aguguugauu     2160

uuuccaaaug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  6913
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6913
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

gaagaggugc agacacgagu guuggaauag ugggauuguu acuaacaaca gcaauggcug      120

cagagguuac cagaagagga ucugcauauu auauguauuu agacagaaac gaugccggug      180

aagcaauaag uuucccaacg acucuaggaa ugaauaaaug cuacauacaa auuauggacu      240

uaggacauau gugcgaugca acaaugagcu augaaugccc aauguuagau gagggaguag      300

aaccagacga uguagauugu ugguguaaua caacaucaac uuggguaguu uacgguacuu      360

gccaucauaa aaaaggagaa gcaagaagau caagaagagc aguaacauug ccuagucauu      420

caacacgcaa acuccaaaca agaucacaaa caugguugga aucaagagaa uacacaaaac      480

auuuaauaag aguagaaaau uggaucuuuc gaaauccagg auuugcacua gcagcagcag      540

caauagcaug guuauuagga aguucuacau cacaaaaagu aauauaccua gucaugauac      600

ucuugauagc cccugcauau ucuauaagau gcauaggagu uaguaauaga gauuucguug      660

aaggaauguc cggaggaacu uggguugaua uagucuuaga acacggagga uguguaacug      720

uaauggcaca agauaaacca acuguagaua uagaauuagu uacaacaaca guaucuaaua      780

uggcagaggu acgaucauac uguuaugagg ccaguauaag ugauauggca uccgacucac      840

gauguccaac acaaggagaa gcuuaccuug auaaacaaag ugauacgcaa uauguaugua      900

aaagaacauu aguagaucga ggauggggca auggcugcgg auuauuuggc aaaggauccu      960

uaguaacaug ugcaaaauuc gcauguagca aaaaaaugac aggaaaauca auacaaccag     1020

aaaauuuaga auauagaaua augcuaucag uacauggauc acaacauagc ggaaugauag     1080

uuaacgauac cggacaugaa accgacgaaa auagagcaaa aguagaaaua acaccaaaca     1140

gcccaagggc agaagcaaca uuaggcggau uuggauccuu agguuuagac ugugaaccac     1200

gcacugguuu ggauuuuagu gauuuauacu accuuacaau gaacaauaaa cauugguuag     1260

uccacaaaga augguuucau gauauaccau uaccauggca ugcuggugca gauacuggga     1320

ccccacauug gaauaauaaa gaagcauuag uagaguuuaa agaugcacau gcaaaaagac     1380

aaacagucgu cguccuaggg agucaagaag gagcaguaca cacagcacua gcaggagccc     1440

uugaagcaga aauggacgga gcaaaaggaa gauuaaguuc aggacauuua aagugucgcc     1500

uuaaaaugga caaauuaaga cuaaaaggag ucaguuauuc gcuauguacc gcagcguuca     1560

cguuuacaaa aauuccggca gaaacucuac augguacugu uacuguagag guccaauaug     1620

caggcacaga cggaccaugu aaaguaccag cucaaauggc cgucgacaug caaacacuca     1680

cuccaguagg aagauuaaua acagcaaauc caguaauuac agaaucaaca gaaaauagua     1740

aaaugauguu agaauuggau ccacccuuug gagacaguua uauuguaaua ggagucggag     1800

aaaaaaaaau aacacaucau uggcaucgau caggaucaac gauaggaaaa gcauuugagg     1860

caaccgucag aggagcuaaa agaauggcag uucuaggaga caccgcaugg gauuucggau     1920

cagucggagg ggcacuaaau ucucucggua aagggauaca ucaaauauuu ggagccgcau     1980

uuaaauccuu auucggaggu augaguuggu uuucacaaau ccuaauagga acauugcuaa     2040

uguggcuggg acuaaauacg aaaaauggua guauaucauu gaugugcuua gcucuaggag     2100

guguauuaau cuuucuuagc acagcaguuu cggcagacgu cggauguagu guagauuuca     2160

guaaaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6914
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6914
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

gaggagccga cacuucagug ggaauuguag gauuauuauu aacaaccgca auggcagcag      120

aaguuacaag aaggggauca gcauauuaua uguaucuuga uagaaaugac gcaggugaag      180

ccauuaguuu ccccacaacc uuagguauga auaaaugcua uauacaaaua auggauuuag      240

gacauaugug ugaugcaacc augaguuacg aaugcccaau guuagacgaa gggguugaac      300

ccgaugaugu agauugcugg uguaacacua caaguacaug gguaguauau ggcacauguc      360

aucacaaaaa aggugaagca agaagaucaa gacgagcagu uaccuuaccu agccauagua      420

cacgcaaauu acaaacaaga ucacagacuu gguuagaaag uagggaauau accaaacacu      480

uaauaagagu ugaaaauugg auauuuagaa aucccggauu ugcauuagca gcagcagcaa      540

uagcgugguu acuaggcagc ucaacuaguc aaaaaguaau auaucuagua augaucuuau      600

uaauagcucc agcauauuca auaagaugua uaggagugag caauagagau uuuguagaag      660

gaaugagugg agguacaugg guagacauag uacuagaaca cggaggcugu guaacaguca      720

uggcacaaga uaaaccaaca guugauaucg aauugguaac aacaacagua agcaacaugg      780

cggaaguaag aagcuacugc uaugaagcua guauaagcga cauggcaagu gauucaagau      840

guccuacaca aggagaagcg uaucuagaua aacaaaguga uacacaauau guuuguaaaa      900

gaacauuagu agauagagga uggggaaaug gauguggauu guuugguaaa gggucauugg      960

ucacuugcgc uaaauuugca uguaguaaaa aaaugacagg aaaaagcauu caaccagaaa     1020

accuagaaua uagaauaaug cuuagugucc auggaucaca gcauucaggu augauaguaa     1080

augacacagg acaugaaaca gacgaaaaua gagcaaaagu agaaauaaca ccgaacagcc     1140

caagagcaga agcuacuuua ggaggauuug gaaguuuagg acuagacugu gaaccaagaa     1200

caggcuuaga uuuuagugac uuauauuauu ugacgaugaa uaauaaacac ugguuaguac     1260

acaaagaaug guuccaugau auaccauugc cauggcaugc aggugcagau acaggaacac     1320

cacacuggaa uaacaaagag gcccugguag aguucaaaga ugcacaugca aaaaggcaaa     1380

caguuguagu auuaggaucu caagaaggug caguucacac ugcccuagcc ggagcauuag     1440

aagccgaaau ggauggagcu aaaggaagau ugaguagcgg acaucuuaaa ugcagauuaa     1500

aaauggauaa auuaagacuc aaagguguaa gcuauaguuu auguacagca gccuuuacau     1560

uuacuaaaau acccgcagaa acacuacaug gaacaguuac aguugaagua caauacgcag     1620

gaacggaugg uccauguaaa guaccugcuc aaauggcagu agacaugcag acacuaacgc     1680

cgguagguag auuaauaaca gcgaaucccg uuauaacuga aucaacagaa aauucaaaaa     1740

ugaugcuaga auuagaucca ccuuuuggag acagcuauau aguaauagga guaggagaaa     1800

aaaaaauaac acaucacugg cauagaucag gcagcacaau ugguaaagcu uuugaagcua     1860

caguucgagg agcaaaaaga auggcaguac uaggagauac agccugggau uuuggaagcg     1920

uagguggagc uuuaaacucu uugggaaaag guauacauca aauuuuugga gcugcuuuua     1980

agaguuuguu ugguggaaug ucgugguuua gucaaauauu gauaggcaca uuacucaugu     2040

gguuaggacu aaauacaaaa aacggaucaa uaagucuaau guguuuagca cuaggaggag     2100

uauuaauauu uuuaucuaca gcaguaagcg cagacguugg auguucagua gauuucagua     2160

aaugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6915
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6915
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaggg       60

gagcagauac aucaguagga auaguaggau uauuauuaac aacagcaaug gcagcagaag      120

uuacaaggag gggaagugcc uacuacaugu acuuagauag aaacgaugca ggagaagcaa      180

uuaguuuucc uacaacauua ggaaugaaua aaugcuauau acaaauaaug gacuuagguc      240

auaugugcga cgcaacuaug ucuuacgaau gcccaauguu agaugaagga guagaaccag      300

acgauguaga cuguuggugu aauaccacaa guacgugggu aguauaugga acuugucauc      360

acaagaaagg agaagcaaga agauccagga gagcaguaac uuuaccauca cacucaacga      420

gaaaauuaca gacaagaagc caaacauggu uagaaucaag agaauauaca aaacacuuaa      480

ucagaguaga aaauuggaua uuucgaaauc caggauucgc acuugcggca gcugcuauag      540

cauggcucuu aggaaguucu accagccaaa aaguaauuua uuuaguaaug auacuuuuaa      600

uagcacccgc auauucaaua agguguaucg gaguuucuaa uagagauuuu guggaaggaa      660

ugucaggcgg aacuugggug gacaucguuu uagaacacgg aggauguguu acaguaaugg      720

cucaagauaa accaacugua gauauagaau uaguaacuac gacaguguca aauauggcag      780

aaguaagauc uuauuguuau gaggcuucaa ucagugacau ggcaucagau aguagauguc      840

caacacaagg agaagcauau uuagacaaac aaagugacac acaauauguc uguaaaagga      900

cauuaguaga cagagggugg ggaaauggau guggacucuu uggaaaagga ucucuaguaa      960

cuugcgcuaa auuugcaugu ucaaagaaaa ugacaggaaa aucaauacaa ccagaaaauc     1020

ucgaauauag aauuauguua ucaguacaug gcucacaaca uaguggaaug auaguuaaug     1080

auacaggaca ugaaacagau gaaaacagag caaaaguaga aauuacaccu aacucaccua     1140

gagccgaagc cacacuaggu gguuuuggaa guuuaggacu agauugugaa ccaaggacag     1200

gucuagauuu uagcgacuua uauuauuuaa caaugaauaa uaaacauugg uugguccaua     1260

aagaaugguu ccacgauauu ccauuaccuu ggcaugcagg agcagauaca ggaacaccac     1320

auuggaauaa caaagaagca uuaguugaau uuaaagaugc acaugcuaaa agacaaacug     1380

uaguuguacu uggaucccaa gaaggagcag uucauacagc auuagcgggu gcacuugaag     1440

cagaaaugga uggagcaaaa gguagacuau caagcggaca ucugaaaugu cgacuaaaaa     1500

uggauaaacu gagauugaaa ggaguuaguu auaguuuaug cacagcagca uucacauuua     1560

caaaaauccc agcagaaaca uuacauggaa caguaacugu agaaguacaa uaugccggaa     1620

cugauggacc cuguaaaguu cccgcacaga uggcgguaga caugcagaca uuaacuccag     1680

uagguagauu aauaacggca aauccuguca uaacggaauc uacagaaaau ucaaaaauga     1740

uguuggaauu agacccacca uuuggagauu ccuacauagu uauaggaguc ggugaaaaga     1800

aaauaacaca ucauuggcac aggagcggaa guaccauugg uaaggcuuuu gaagcaacag     1860

uaagaggagc aaaaagaaug gcaguuuugg gagauacagc augggauuuc gguaguguug     1920

gaggagcucu uaauucuuua ggcaaaggaa uacaucaaau auucggagca gcuuuuaaaa     1980

gucuauucgg agggaugagu ugguuuagcc aaauacuaau uggaacgcua cugauguggu     2040

uaggacucaa cacuaaaaac gggagcauua guuuaaugug ccuagcauua gguggaguac     2100

uaauauuucu aucaacagca guuucagccg augucggcug uucaguagau uuuuccaaau     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6916
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6916
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggug       60

cagauacauc aguuggaaua guaggacugc uauuaacaac agcaauggcg gcagaaguaa      120

caagacgggg aucagcuuau uacauguauc uagauagaaa ugaugcagga gaagcaauau      180

cauucccaac gacacuagga augaacaaau guuacaucca aauaauggac cuaggacaua      240

ugugcgacgc cacuaugagc uaugaaugcc caaugcuaga ugaaggagua gaaccggaug      300

auguugacug uugguguaau acgacaucaa cuuggguagu auacggaacu ugucaucaua      360

agaaaggaga agcaagacga aguagacgag caguaacacu accaucacac aguacaagaa      420

aauuacaaac aagaagucag acauggcuug aaagcagaga auauacaaaa cauuuaauaa      480

gaguagaaaa uuggauuuuu agaaauccag guuucgccuu agcugcagcu gcgauagcau      540

ggcucuuggg aucuagcacu ucacaaaaag uaauauacuu aguaaugauu uuacuaauug      600

caccagcaua uucaaucaga uguauuggag ucagcaauag ggacuuugua gaaggcaugu      660

cagguggaac guggguagau aucguacuag aacacggagg gugcguaaca guaauggcac      720

aggauaaacc aacgguagac auagaauuag uaacgacaac gguaucaaau auggcagaag      780

uaagaaguua cuguuacgag gcaucuauua gugacauggc uucagacuca cgauguccaa      840

cacaaggaga agcauauuua gauaaacaaa gugauacgca auacguaugc aaaagaacau      900

uaguagacag aggguggggu aauggaugcg guuuauuugg aaaaggauca uuaguaacau      960

gugcaaaauu ugcauguucu aagaaaauga caggaaaaag uauccaacca gagaauuuag     1020

aauacaggau uauguuaagu guacacggau cacaacauuc agggaugaua guaaaugaca     1080

caggacauga aacugaugaa aaucgagcaa aaguagaaau aacucccaau ucaccaagag     1140

cugaagcuac auuaggggga uuuggaucac uaggacuaga uugugaaccg agaacaggau     1200

uagauuuuag ugauuuauau uauuuaacaa ugaacaauaa acacugguua guacauaaag     1260

aaugguuuca ugacauacca uuaccguggc acgcaggggc agauacugga acaccacauu     1320

ggaauaauaa agaggcacug guugaguuca aagacgcuca cgcaaaaaga caaacaguag     1380

ucguguuagg cucucaggaa ggagcaguac auaccgcguu agcaggcgca cuagaagcag     1440

aaauggaugg cgcuaaaggc agacuuagca gcggacacuu gaaaugcaga cuaaaaaugg     1500

acaaauuacg guuaaaaggc gugaguuauu cauuauguac agccgcauuc acauucacaa     1560

aaauaccugc ugaaacuuua caugggacag ugacgguaga aguacaguau gcggggacag     1620

acggaccuug uaaaguaccu gcacaaaugg caguagauau gcaaacuuua acaccaguag     1680

gaagauuaau aacagcaaau ccaguaauua cagaaaguac agaaaauagu aaaaugaugc     1740

uagaauuaga cccaccuuuu ggcgacucau auauagucau aggaguaggu gaaaagaaaa     1800

uaacacacca uuggcacagg aguggaucua caauaggaaa agcauuugaa gccacaguaa     1860

gaggagccaa aagaauggca guacuaggag acacagcuug ggauuuugga agcgucggag     1920

gagcacuaaa uaguuuagga aaaggaauac aucaaauauu cggagcagcc uucaaaucau     1980

uauuuggugg uauguccugg uuuagucaaa uauuaauagg aacauuauua augugguuag     2040

gacuaaauac uaaaaacggu aguaucucac uuauguguuu agcccugggu ggaguauuaa     2100

uauuuuuauc aaccgcugua ucagcugaug uuggauguuc aguagauuuc ucaaaaugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6917
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6917
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcag       60

auacauccgu aggaauagua ggacuuuuau uaacaacagc aauggcagcg gaaguaacaa      120

gaagagguuc agccuauuac auguauuugg auaggaauga cgcaggagaa gcaauuagcu      180

ucccuacaac acuugggaug aacaaauguu auauacaaau cauggaccua ggacauaugu      240

gugaugcaac uaugaguuac gaauguccaa ugcuugauga gggaguagaa ccagacgacg      300

ucgauuguug gugcaauacu acaaguacuu ggguaguaua ugguacaugu caucauaaaa      360

aaggagaagc aagacgcuca aggagagcag uaacauuacc uagucacuca acaagaaaac      420

ugcaaacacg aagucaaaca uggcucgaga gccgagaaua uacaaaacau cuaauuagag      480

uagagaacug gauauucaga aauccaggau ucgccuuagc ggcagcagca auagcauggu      540

uacuaggaag uucaacauca caaaaaguaa uauaucuagu caugauuuua uuaauagcuc      600

cugcauauuc uauacgaugu auaggaguca guaauaggga uuuuguagaa ggaaugagug      660

gagguaccug gguagauauu guauuagaac acggaggcug uguaacuguu auggcacaag      720

auaaaccuac aguagauaua gaacuaguaa cgacaacagu gagcaacaug gcugaaguga      780

gaucauauug uuaugaagcu agcauaagcg auauggccag ugauucuaga uguccaaccc      840

aaggagaagc auacuuagau aagcaaucug auacccaaua uguaugcaaa agaacacuag      900

uagaccgagg auggggcaau ggaugcgguc uauucggaaa aggaagcuua gugacaugug      960

caaaauuugc auguuccaaa aaaaugacag gcaaaagcau acaaccagaa aauuuagaau     1020

acaggauaau gcuaucagua cauggaucuc aacauagcgg aaugauagua aaugauacag     1080

gacacgaaac ugacgaaaau agagccaaag uagagaucac acccaacagu ccaagagcag     1140

aagcaacgcu aggaggauuu ggaagccucg gauuagauug ugaaccaaga acaggacuag     1200

auuucagcga ucuuuacuau uuaaccauga acaacaaaca uugguuagua cauaaagagu     1260

gguuucauga uauaccguua ccuuggcaug caggagcaga uacaggaaca ccacacugga     1320

auaauaaaga agcccuugua gaauuuaaag acgcacaugc aaaaagacaa acgguaguug     1380

uauuagguag ucaagaagga gcaguacaua cagcacuagc uggugcauua gaagcagaaa     1440

uggacggagc aaaaggaaga uuaagcagcg gacaucuaaa gugcagacua aaaauggaua     1500

aacuucgccu aaaaggagua ucauacucau uauguacugc cgcuuuuaca uucaccaaaa     1560

uaccugcuga aacuuuacac ggaacaguca cuguagaagu acaauaugca gguacagaug     1620

guccauguaa agugcccgcu caaauggcag uagauaugca aacacuaaca ccuguaggaa     1680

gauuaauaac agcaaacccu guaauaacag aaucaacaga aaacucgaaa augaugcucg     1740

aacuagaccc accguuugga gauaguuaca uuguaauugg uguaggagaa aaaaaaauua     1800

cacaucauug gcaccguuca gggaguacua uaggaaaagc auuugaagca acuguaagag     1860

gagcaaaaag aauggcagua uuaggugaua cagccuggga cuucgguagu guaggaggag     1920

cauugaacuc ccuaggaaaa ggcauucauc aaauauuugg agcagcauuu aaaaguuuau     1980

uuggaggaau gucaugguuu agucaaauau uaauaggcac gcuacuuaug ugguuaggac     2040

uaaauacaaa aaacggaagu auuagccuaa ugugcuuagc uuuagggggc guguuaauuu     2100

uuuugagcac agcaguuagc gcagacguag gauguagugu ugauuuuuca aaaugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6918
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6918
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

cuaguguagg aauaguaggu cuacuacuaa caacagcaau ggcagcagaa guuacucgaa      120

gagguagcgc auauuauaug uauuuagaua gaaaugacgc aggagaagcu auaaguuucc      180

caacaacacu aggaaugaac aaauguuaca uucaaauaau ggaccuggga cauaugugcg      240

augcaaccau gucuuacgaa uguccuaugc uugacgaagg aguagaaccu gaugauguug      300

acuguuggug uaauacaacc agcacuuggg uaguauaugg aacaugucau cauaaaaaag      360

gggaagcaag acgaucaaga agggcaguga cauugccaag ucacucaacu agaaaauuac      420

aaacuagauc acaaacaugg uuggaaucaa gagaauacac aaaacauuua auuagagugg      480

aaaauuggau auuccgaaac ccaggauucg cauuagcggc agcagcaaua gcuugguuau      540

ugggaagcuc aacgagucaa aagguuauau accuagugau gauacuauua auagcuccag      600

cauauuccau aagaugcaua ggaguaagua accgagacuu uguagaagga augaguggag      660

gaacaugggu agauauagua uuggaacaug gaggaugugu aacaguaaug gcacaagaua      720

aaccaacagu agauauagaa cuaguuacaa caacaguuuc aaauauggcc gaaguaagau      780

cguacuguua ugaggcuagc auaucagaca uggcaucuga uucaagaugu ccaacucaag      840

gcgaagcaua cuuagauaaa cagagugaca cgcaauaugu augcaaaaga acacucgugg      900

auagaggaug ggguaacggg uguggccugu ucggcaaagg uagucuagua acaugugcua      960

aauuugcuug uucaaaaaaa augacaggaa aaucaauaca accggaaaac cuagaauaca     1020

gaauaauguu aaguguccac gggagucaac auucaggaau gauuguuaau gauacagguc     1080

augagacuga ugaaaauaga gcaaaaguag agaucacacc aaauucacca cgagcagaag     1140

caacacuagg ugguuuugga aguuuaggau uagauuguga accaagaaca ggauuagauu     1200

uuucagacuu guauuacuua acaaugaaua auaaacacug guuaguccac aaagaauggu     1260

uucaugauau uccacugcca uggcaugcag gagcagauac aggcacacca cacuggaaua     1320

acaaagaagc auuaguugaa uuuaaagaug cacacgcuaa aagacaaaca guagucguau     1380

uaggaaguca agaaggagcc guucacacag cacuagcagg ugcauuagaa gcugaaaugg     1440

acggagcuaa agguagauua ucaaguggac accuaaaaug cagacugaaa auggacaaau     1500

uaagacuaaa aggaguaagu uauagcuuau guacagcggc auuuacauuu acaaaaauac     1560

cggcagaaac auuacauggu acaguaacug uagaaguaca auacgccgga acagauggac     1620

caugcaaagu accugcucaa auggccgucg auaugcaaac ccuaacaccg guuggaagac     1680

ucauaacagc aaauccagua auaacagagu caacagaaaa uaguaaaaug auguuagaau     1740

uagacccacc auuuggugac ucguacauag ugauaggagu uggugaaaaa aaaauaacac     1800

accauuggca cagaagugga agcacuauug gaaaagcauu ugaagcaacu guuagaggcg     1860

caaaaagaau ggcaguucuu ggagauacag caugggauuu uggaaguguu ggaggagcau     1920

uaaacaguuu aggaaaaggc auacaucaaa uuuuuggagc agcuuuuaaa ucacuauuug     1980

gaggaauguc augguuuuca caaauauuaa uugguacuuu acuaaugugg uuaggacuaa     2040

auacuaaaaa ugguaguaua aguuuaaugu gucuagcauu aggcggaguc uuaaucuucu     2100

uaucaacagc aguaagcgca gacguagggu guaguguaga cuucucaaaa ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6919
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6919
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguuggaau aguagguuua cuauuaacaa ccgcaauggc agcagaagug acaagaagag      120

gaagugcaua cuauauguac uuagaucgua acgaugcagg ugaagcuaua ucuuuuccaa      180

caacguuagg aaugaauaaa uguuacauac aaauaaugga cuuaggacau augugugacg      240

caacuauguc auaugaaugu ccaauguuag augaaggagu ugaaccagac gacguagauu      300

guugguguaa uacuaccagu acauggguag uauauggaac augccaucau aaaaaaggag      360

aggcuagaag aaguaggaga gcaguaacau uaccaucuca uucaacaaga aaacuucaaa      420

cacgaucaca aaccuggcua gaaagccgag aauacacaaa acaucuaaua cgaguggaaa      480

auuggauauu uagaaaucca ggauucgcac uagcugcagc cgcuaucgca uggcuacuag      540

guaguucaac aucacaaaaa guaauuuacu uaguaaugau ccuacucauu gccccggcau      600

auucaauaag augcaucggg guuagcaaca gagacuuugu ugaaggcaug ucgggaggca      660

cauggguaga uauuguacuu gagcacggag ggugcguaac uguuauggcu caagauaaac      720

caacaguuga cauagaacua guaacaacaa caguaucaaa cauggcggaa guaagaaguu      780

auugcuauga agcaagcaua ucugacaugg caucagauuc uagaugucca acacaaggag      840

aagcuuauuu agauaaacaa ucagacacac aauacguaug uaaaagaaca uuaguagaca      900

gaggcugggg aaauggaugu ggauuguuug gaaaaggauc auugguaaca ugugccaaau      960

ucgcauguag uaaaaaaaug acuggaaaaa guauccaacc agaaaauuua gaauaucgaa     1020

uuauguuaag cguccauggu ucccaacauu cagguaugau aguaaacgau acaggacacg     1080

agacugauga aaauagagca aaaguggaaa uaacuccaaa uaguccaaga gcagaagcaa     1140

cuuuaggugg uuuuggaucc cucggauugg auugugaacc aagaaccgga uuagacuuca     1200

gugaucuaua cuacuuaaca augaauaaua aacacuggcu uguccacaaa gaaugguucc     1260

acgacauccc auuaccaugg cacgcaggcg cagauacagg caccccacau uggaauaaua     1320

aagaagcacu aguagaauuc aaagacgcac augcaaaaag acaaacggua guaguauuag     1380

gaagucaaga aggagcugua cauacagccc uagcuggagc acuagaagca gaaauggacg     1440

gagcaaaagg uagauuaagu aguggacacc ugaaaugcag auuaaaaaug gauaaacuaa     1500

gguuaaaagg aguuagcuau ucuuuaugua cugcagcauu uacauucacu aaaauaccag     1560

cagaaaccuu acauggaaca gugacaguag aaguacaaua ugcaggaacu gauggaccau     1620

guaaaguacc agcacaaaug gcaguugaua ugcaaacauu aacuccagua gggagacuua     1680

uaacagcaaa uccaguaaua accgaaucaa cagaaaauag caaaaugaug uuagaauuag     1740

acccaccuuu cggcgacagc uacauaguca uaggaguugg ugaaaaaaaa auaacccauc     1800

auuggcauag aaguggaagc acaauaggca aagcauuuga agcaacuguc agaggggcca     1860

aaagaauggc aguucuagga gauaccgcau gggacuucgg aaguguagga ggagcacuaa     1920

acaguuuagg aaaaggaaua caucagauau uuggggcagc auucaaaagu cuauuugggg     1980

gcaugagcug guuuucacaa auuuuaauug gaacauuguu aauguggcua ggauuaaaua     2040

caaaaaaugg uagcauaagc uuaauguguc uagcucuagg aggaguacug auuuuuuuaa     2100

gcacagcugu uagugcugau guaggguguu cuguagauuu uucaaaauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6920
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6920
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguugguau agugggguua uuauuaacga cugcaauggc agcagcagua acaagaagag      120

gaucagcuua uuauauguau uuagacagaa augaugcagg ggaagcaauc ucauuuccca      180

ccacucuagg uaugaauaaa uguuauauuc aaauaaugga ucucggucau augugugaug      240

cuacuaugag uuaugaaugu ccaauguuag augaaggagu ugaaccagau gaugucgacu      300

guuggugcaa uacaacaagu acuuggguag uauauggaac cugucaucau aagaaaggag      360

aagcuagacg uaguaggaga gcaguaacac uuccaaguca uucaacaaga aaacuacaaa      420

cuagaagcca aacaugguua gaaaguagag aauauacaaa acaccuaaua agaguagaaa      480

acuggauuuu uagaaaccca ggauuugccc uggcagcugc ugcuauagcc ugguuacugg      540

gauccaguac aucacaaaaa guaauauacu uaguaaugau auuauugaua gcucccgcuu      600

auagcauaag auguauuggc gucucuaaua gagauuucgu agaagggaug agugguggga      660

cauggguaga uauaguauua gaacacggag guugcguaac aguaauggca caagacaaac      720

caacaguaga uauagaauua gugacaacaa cagugagcaa uauggcugaa guacgaucau      780

auugcuauga agcaucaauc ucagauaugg cuucagacuc aagaugucca acgcaaggag      840

aagcauaucu agacaaacaa agugauacac aauacguaug uaaaagaaca uuaguagaua      900

gagguugggg uaaugguugu ggacuauuug gaaaaggcuc cuuaguuaca ugugcuaaau      960

ucgcuugcuc caagaaaaug acaggaaaau caauacaacc ugaaaaucuu gaauacagaa     1020

uaaugcuaag uguacaugga agccaacaua gugggaugau aguaaaugau acgggacaug     1080

aaacugauga aaauagagca aaaguagaaa uaacuccaaa uagcccaaga gcagaagcaa     1140

cuuuaggcgg auucggcagc cuuggauuag auugugaacc aagaacaggc uuagauuuua     1200

gcgacuuaua uuacuuaaca augaauaaca aacauugguu aguccauaaa gaaugguuuc     1260

acgauauacc auuaccaugg caugcgggug cugauaccgg uacaccacau uggaauaaca     1320

aagaagcauu gguagaguuc aaagacgcac acgcaaaaag gcaaacaguc guagucuugg     1380

gaagccaaga aggagccgua cauaccgcau uagcaggagc acuagaagca gaaauggacg     1440

gagcaaaagg aagauuaagc aguggacauu uaaaaugucg auuaaaaaug gauaaacuua     1500

gauuaaaagg agucucauau ucacuuugua cagcagcauu uacuuuuaca aaaauaccug     1560

cagaaacauu acaugggaca gugacagugg aaguacaaua ugcaggaaca gauggaccau     1620

guaaaguacc agcucaaaug gcuguugaua ugcaaacacu aacaccagua ggacgacuaa     1680

uaacugcuaa uccaguaaua acagaaucaa cugaaaauag caaaaugaug cuagaacuug     1740

acccgccuuu uggugacagu uauauaguua uaggugucgg ugaaaagaaa aucacacauc     1800

acuggcauag gaguggguca acaauaggga aagcguuuga agcaacaguc aggggugcaa     1860

aaagaauggc uguacuagga gauacagcau gggauuuugg aaguguagga ggggcauuga     1920

auagucuagg aaaagguaua caucaaauau uuggggcagc auuuaaaagu cuauuuggcg     1980

gaaugucaug guuuucacaa auucuaauag gaacuuuacu uauguggcuc ggauugaaua     2040

cgaaaaaugg aucaaucagc uuaauguguu uagcuuuagg aggugugcua auauuuuuaa     2100

guacagccgu aucagccgau guaggauguu cuguagauuu uaguaaauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6921
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6921
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gagcagauac aaguguuggg auaguaggac ugcuucuaac uacugcaaug gccgcugcgg      120

uaacgagacg aggaagugca uauuacaugu auuuagacag aaacgaugca ggagaagcua      180

uaaguuuucc aacaacgcua gguaugaaca aaugcuacau acagauaaug gaucuaggac      240

acauguguga cgcaacuaug ucauacgaau gcccaauguu agaugaaggu guagaaccag      300

augaugucga cuguuggugc aacacaacuu cgacaugggu aguauaugga acaugucauc      360

auaaaaaagg agaagcacgc cgaucaagaa gggcagucac auuaccauca cacaguacaa      420

ggaaauuaca aacaagaagu caaacauggc uugaaucaag agaauauaca aaacacuuaa      480

uuaggguaga aaauuggauu uucagaaauc caggauuugc auuagcagcc gcagcaauag      540

cuugguuauu aggaaguagu acaagccaaa aaguaauuua uuuaguuaug auacuuuuaa      600

uagcuccagc auauucaaua cgauguauag guguaucaaa cagagauuuu guugaaggaa      660

ugaguggagg aacaugggua gauauaguuc uagaacaugg agguugugua acaguaaugg      720

cacaggacaa accaacagua gacauagaau uaguaacaac aacaguuuca aauauggcag      780

aaguaagauc uuauuguuac gaagcaagua uaagcgauau ggcuagcgau ucacgauguc      840

ccacacaagg agaagcuuau cuagauaaac aaucagauac acaauacgua uguaaacgaa      900

cauugguaga cagaggaugg ggaaauggau guggauuauu uggaaaaggu agccuaguaa      960

caugcgcaaa auucgccugu aguaaaaaaa ugacaggaaa aucaauucaa ccagaaaacu     1020

uagaauauag aauaaugcua uccguucacg gcucgcaaca uucagggaug auuguaaaug     1080

auacaggaca ugaaacagau gaaaacaggg cuaagguaga aauaacuccc aauucaccaa     1140

gagcugaagc aacauuaggc ggguuuggau cacucggucu agacugugaa ccacgaacug     1200

gauuagauuu uucagaucuc uacuaucuga cuaugaauaa uaaacauugg uuaguccaua     1260

aagaaugguu ucacgauaua ccacuacccu ggcaugcagg ugcagauacu ggaacaccuc     1320

acuggaauaa uaaagaagcu cuuguagagu uuaaagaugc acaugcaaaa agacaaacag     1380

uaguaguauu aggaagucaa gaaggagcag uacacacagc auuagccggu gcucuagaag     1440

cagaaaugga cggagcaaaa ggcagacucu caaguggaca uuuaaaaugu cgauuaaaaa     1500

uggacaaauu aagguuaaaa gguguaaguu acagucuaug uacagcugca uuuacauuua     1560

caaaaauacc ugcugaaacg cuccauggaa cagucacagu ugaaguccaa uaugccggaa     1620

ccgauggccc auguaaaguc ccugcacaaa uggcgguaga caugcaaaca uuaacaccag     1680

uugguagauu aauaaccgca aauccgguua uaacagaaag uacagaaaau uccaaaauga     1740

uguuagaauu agauccacca uuuggagacu ccuauauagu aauuggagua ggagaaaaaa     1800

aaauaacaca ucauuggcac agaucagguu caacaauagg aaaagcguuc gaagcgacag     1860

ugagaggagc aaaaagaaug gcuguauuag gagauacagc uugggacuuu ggguccguag     1920

gcggugcacu uaauucacuu ggaaaaggca uacaucaaau uuucggggcg gcuuuuaaau     1980

cacuuuucgg aggaauguca ugguuuucac agauauuaau aggaacauua uuaauguggu     2040

uaggacuuaa uacaaaaaau ggaagcauua guuuaaugug ccuagcgcug ggagguguac     2100

uuauauuucu uucaaccgca guaagugcug auguagggug uucuguagac uuuuccaaau     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6922
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6922
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gacugucauc gagaaaaaga agaagccaug auguccuaac aguucaauuu cuaauauuag      120

ggauguuacu aaugacggga ggaguaacua gacguggauc agcauauuau auguaccuag      180

auagaaacga ugcuggugaa gcaauaucau uuccaacaac cuuaggaaug aauaaauguu      240

acauucaaau uauggauuug ggucauaugu gcgaugcaac cauguccuau gaauguccaa      300

uguuagacga aggaguggaa ccagaugaug uagauuguug gugcaauaca acgucaacuu      360

ggguaguaua uggaacuugu caucauaaga aaggagaagc aagaagaucu agacgagcag      420

uaacauuacc aucacauuca acuagaaaac uacaaacaag aagccaaaca ugguuagaga      480

guagagaaua caccaaacau cuaauaaggg uugaaaauug gauauuucgu aauccugguu      540

ucgcauuagc agcagcagcu auagcauggu uauuaggaag caguacauca caaaaaguaa      600

uauacuuagu caugauauua cuaaucgcac cagccuauuc aauaagaugu auaggaguua      660

guaauagaga uuuuguggaa gguaugagug guggaacaug gguugacauc guacuagaac      720

auggagggug uguuacugua auggcacaag auaaaccgac aguugauauc gaauuaguga      780

caacaacagu aucuaauaug gcagaaguaa gaagcuauug uuaugaagcu agcauauccg      840

auauggcauc agacagcaga ugucccacac agggagaagc auaucuugau aaacagucag      900

acacacagua uguaugcaaa agaacauuag uagacagagg guggggaaac ggaugugguu      960

uauuuggaaa aggaucauua guaaccugug cuaaauuugc augcagcaag aaaaugacug     1020

gcaaaaguau acaaccggaa aaucuagaau accgaauaau gcucaguguu cacggaucac     1080

aacauucagg uaugauugua aacgauacug gacaugaaac agaugaaaac agagcaaaag     1140

ucgaaauaac uccaaacagu ccacgagcag aagcuacuuu aggcgguuuu ggaucccuug     1200

gauuagauug ugagccacga acuggauuag auuucaguga uuuauauuau uuaacaauga     1260

auaauaaaca cugguuagua cacaaagaau gguuccauga uauaccauua ccuuggcaug     1320

caggagcuga uacagggaca ccacacugga auaacaaaga agcccuagua gaauuuaaag     1380

augcacaugc gaaacgacaa acaguuguug ucuuaggauc ucaagaaggu gcaguacaua     1440

cagcauuagc aggagcccua gaagcagaaa uggacggugc uaaagggcga uuaucuagcg     1500

gacauuuaaa auguagauua aaaauggaua aauuaagacu aaaagguguu agcuauaguu     1560

uauguacagc cgcauucaca uucacuaaaa uaccugcaga gacucuacau ggaacaguaa     1620

cuguagaagu acaauaugca gguacugacg gaccuugcaa aguuccagca caaauggcag     1680

uugacaugca aacacuaacu ccaguaggaa gauuaauaac ggcgaaccca guuauaacag     1740

aaucaacuga aaauagcaaa augaugcuug aacuagaucc accauuugga gacucauaua     1800

uaguaauagg aguaggcgaa aagaaaauaa cacaucacug gcauagaagu gguagcacua     1860

uugguaaagc auuugaagca acuguaagag gugcaaaaag aauggcagua uugggagaua     1920

cugcauggga uuuuggauca guaggcgggg cacuaaauuc acuugggaaa ggaauacauc     1980

aaauauuugg agcagcauuu aaaucuuuau uuggaggcau gucaugguuc ucacaaauau     2040

ugauuggaac ccuacuuaug uggcuagguu uaaacacuaa aaauggauca auuucauuga     2100

ugugcuuagc auugggagga guauuaauau ucuuaagcac agcaguaagu gcagacguag     2160

gaugcagugu ugacuuuagu aaaugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6923
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6923
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauuaaguag cagaaaaaga agaagccaug acguguuaac aguacaauuu cugauacugg      120

gaaugcuacu aaugacugga gcaguaacua gaagaggaag cgcauauuau auguacuuag      180

auagaaauga ugcuggugaa gcuauauccu uuccaaccac auugggaaug aauaaauguu      240

auauacaaau uauggacuua ggacauaugu gugaugcuac aaugucauau gaguguccua      300

ugcuagauga aggaguagaa ccagaugacg uagauuguug guguaauaca acgucaacau      360

ggguaguaua uggaacuugu caucauaaga aaggagaagc aagaagauca aggagagcug      420

ugacacuacc uucacauagc acaagaaaac uucaaaccag aagccaaaca uggcucgaau      480

caagagaaua uacuaaacau cuaauacgug uugaaaauug gauauuuaga aacccaggau      540

ucgcacuugc cgcagcagca auagcauggu uauuggguuc gaguacaucu cagaaaguaa      600

uauacuuagu aaugauucua uuaaucgcac cagcauauuc aauaagaugc auaggaguga      660

gcaaccgaga uuuuguugaa ggcaugagug gaggaacgug gguugauaua guucuggaac      720

acggaggaug uguuacagua auggcacaag acaaaccaac aguagacaua gaauugguca      780

ccacaacagu aaguaauaug gcagaaguaa gaagcuacug uuacgaggcc aguauuagug      840

auauggcauc agauuccaga uguccuacac aaggagaagc auauuuagac aaacaaucag      900

auacacaaua uguauguaaa aggacauuag uugacagagg auggggaaau ggaugcggau      960

uauuugguaa aggaucauua gugacaugug cuaaauuugc augcaguaag aaaaugacag     1020

gaaaaagcau acaaccggaa aaucuugagu auaggauaau guuaagugua cacggaucac     1080

aacauucugg aaugauugua aaugauacug gacaugaaac agaugaaaau cgagcaaaag     1140

uagaaauaac ucccaauuca ccaagagcug aggcaacacu aggaggauuu gggagcuuag     1200

gauuagauug ugaaccuagg acuggacuug auuuuaguga ucuauauuau uuaacgauga     1260

auaauaaaca uuggcuagua cauaaagaau gguuucauga caucccauua ccauggcaug     1320

ccggggcaga uacaggaaca ccacacugga auaacaaaga agcguuggua gaauuuaaag     1380

augcacaugc aaaaagacaa acaguuguag uauuaggaag ccaagaggga gcaguacaua     1440

cugcacuugc aggagcauua gaagcagaaa uggauggugc aaaaggaaga cuaucaucag     1500

ggcauuuaaa auguagacuu aaaauggaua aauuaagacu aaaaggcgua agcuauucau     1560

uauguacagc agcauuuacu uucacaaaaa uaccagcaga aacauuacau ggaacaguaa     1620

caguggaagu acaauaugcg ggaacagaug gaccauguaa aguuccagca caaauggcag     1680

uugauaugca aacauuaaca ccaguuggaa gacuaauaac agcaaaucca guaauaacug     1740

aauccacaga aaacucaaaa augaugcuug aacuagaucc accauuugga gauaguuaua     1800

uagugauugg cguuggggag aagaaaauaa cacaucauug gcauagauca ggaucaacaa     1860

uaggaaaagc auuugaagca acuguaagag gagcaaaaag aauggcaguu cuaggagaua     1920

cagcauggga uuuuggaagc guuggaggag cgcuaaauag cuuggggaaa gggauucacc     1980

aaauauuugg agcugcauuu aaaucauuau uuggaggaau gaguugguuu agccaaauac     2040

uaauaggaac cuuauugaug ugguuaggau uaaauacuaa aaacggaagu auaaguuuaa     2100

ugugucuagc acuaggggga guuuuaauau uuuuaaguac agcaguauca gccgauguag     2160

gcuguagugu agauuuuuca aaaugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  6924
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6924
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc aaggaaagag aaaaaaagaa gaggugcaga uacaagcguu gguauaguag      120

gacuacuauu aacaacugca auggcggcag aaguaacaag acgugguuca gcuuauuaca      180

uguauuuaga uagaaacgau gcuggagagg caauaucauu uccuacaacu uuaggaauga      240

auaaaugcua uauacaaaua auggauuuag gacauaugug ugacgcaaca augaguuaug      300

aaugcccuau gcuagaugaa ggaguugaac ccgacgaugu agauuguugg ugcaauacua      360

caaguacaug gguuguauau ggaacauguc aucacaaaaa aggagaggcu agaagaucaa      420

gaagagcagu aacauuaccu ucacacagca cacgaaaacu acaaacaaga ucacaaacau      480

gguuagaauc aagagaauac acaaaacauc uaauuagggu agaaaauugg aucuuuagaa      540

auccuggcuu ugcacuugca gcagcagcaa uagcuugguu auuagguagc aguacaucuc      600

aaaaaguaau auauuuagua augauacuac uaaucgcacc agcauacagc auaaggugca      660

uaggaguauc uaauagggau uuuguagaag gaaugucagg aggaacaugg guugacauug      720

uacuagaaca uggugguugu guaacaguaa uggcgcaaga uaaaccaaca gucgauauag      780

aacuaguaac aacaaccgua ucaaauaugg cugaaguuag aucauauugu uaugaagcau      840

caaucagcga uauggcauca gacagcagau gcccaacuca aggagaagcu uauuuagaua      900

agcaaucaga uacucaauac guauguaaaa gaacacuagu cgauagagga ugggguaacg      960

gaugugguuu auucggcaaa ggaucauuag uuaccugugc caaauuugca uguucaaaaa     1020

aaaugacugg aaaaagcaua caacccgaga auuuagaaua uaggauaaug uugucugucc     1080

auggaucuca acacucaggu augauaguua augauacagg acacgaaaca gaugaaaaua     1140

gggcuaaagu agaaauaaca cccaauaguc cucgagcuga agcaacacuc ggaggauuug     1200

gaagucuagg acuagacugu gaaccaagaa caggacuaga cuuuagugau cuauauuauu     1260

uaacaaugaa caacaaacau uggcuaguac auaaagaaug guuucacgac auaccucuac     1320

cuuggcacgc aggcgcugau acaggaacac cacacuggaa caauaaagaa gcacuaguug     1380

aauuuaaaga ugcucacgca aaaagacaaa cagucgucgu acuaggaagu caagaaggag     1440

cuguacauac cgcgcuagcc ggcgcuuuag aagccgagau ggacggagca aaaggaagau     1500

uaucaucggg acacuuaaaa uguagauuaa aaauggacaa gcuaagauua aaaggagucu     1560

cauauucacu augcacagca gcauuuacau ucacaaaaau uccugcggaa acacuacaug     1620

gaacaguaac uguugaagua caauaugcag gaacagaugg acccugcaag guuccugcac     1680

aaauggcugu agauaugcaa acacuaacac caguaggaag acuaauaaca gcaaacccag     1740

uaauaacaga aucuacagaa aauucaaaaa ugauguugga auuagaucca ccauuugggg     1800

auaguuauau aguaauaggu guaggagaaa aaaaaauaac acaucacugg cauagaagug     1860

gcuccacgau aggaaaagca uucgaagcaa caguacgagg agcaaaaaga auggcaguac     1920

uaggagacac agcaugggau uucggauccg uuggaggagc auugaauuca uuagguaaag     1980

guauccacca aauauuugga gcggcauuua aaucguuauu cggaggaaug aguugguuuu     2040

cucaaauccu aauugguaca cuacucaugu gguuagguuu aaacacaaaa aaugguucaa     2100

ucucauuaau gugcuuagcg cuuggaggag uacuaauauu ucucaguaca gcugugagug     2160

cugacguagg uuguugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6925
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6925
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauua       60

uuaaugcaag aaaagaaaag aaaagaagag gugccgauac aagcguagga auaguaggac      120

uacuacuuac aacagcaaug gcagccgaag uaacaagacg cgguucugcu uauuauaugu      180

aucuagacag aaaugaugcu ggagaagcca uuaguuuccc aacaacauua ggaaugaaua      240

aauguuacau acaaauaaug gaccuaggac acauguguga cgcaacaaug ucauaugaau      300

gcccaauguu agacgaaggu guugaaccag acgauguaga uuguuggugu aauacaacau      360

caacaugggu uguuuaugga acaugccauc auaagaaagg cgaggcuaga agaucaagac      420

gagcagucac auuaccuagu cauaguacaa gaaaauuaca aacaagaucu cagacauggc      480

uugaaucaag agaauauaca aaacacuuaa uuagaguuga aaauuggauc uuuagaaacc      540

cugguuuugc acuagccgca gcugcaauag cuuggcuauu aggaucaucu acaucacaaa      600

aaguaauaua uuuagucaug auucuacuaa uagcaccagc auauucaauc agaugcauag      660

gagugagcaa uagagauuuu guggaaggca ugucuggagg gacaugggua gauauuguau      720

uagaacaugg agggugcgua acuguuaugg cacaggauaa accaacagua gacauagaau      780

uaguuacaac aacaguuuca aauauggcgg aaguuagaag uuacuguuau gaagcaucaa      840

uuucagauau ggcaucagau aguagauguc caacacaagg ggaggcuuau uuagauaaac      900

aaagugacac ucaauaugua uguaaacgaa cauuaguaga uagaggaugg ggcaacgguu      960

gugguuuauu uggcaaagga aguuuaguaa caugugcaaa auuugcgugu aguaagaaaa     1020

ugacaggcaa aucaauacaa ccagaaaacu uagaauaucg aaucaugcuu aguguacacg     1080

gcucacaaca uucaggaaug auuguaaaug acacuggaca ugagacagau gaaaacagag     1140

caaaagugga aauuacaccu aacucaccaa gagcagaagc uacguuagga gguuucggau     1200

cucuuggauu agacugugaa ccaagaacag gcuuagacuu cucugaucua uauuaucuua     1260

caaugaauaa caaacauugg uuaguacaua aagagugguu ucaugauaua ccauuaccau     1320

ggcacgcagg agcagauaca gggacgccac auuggaacaa uaaagaagcu cuaguagaau     1380

uuaaagaugc acacgcaaaa agacaaacag uaguuguuuu agguucucaa gaaggagccg     1440

uacacacagc acuagcugga gccuuagaag cagaaaugga cggggcuaaa gguagauuau     1500

caucuggaca cuuaaaaugu agauuaaaaa uggauaaauu aagauuaaaa ggaguaaguu     1560

auagccugug uacagccgca uuuacuuuua ccaaaauacc agcagaaaca uuacauggua     1620

caguaaccgu agaagugcag uaugcaggaa cagaugggcc uuguaaaguc ccagcucaaa     1680

uggcaguaga uaugcaaaca cuuaccccag uaggcagacu uauaacugca aauccaguga     1740

uaacagaauc aaccgagaac uccaaaauga ugcuagaacu agauccacca uucggagaua     1800

guuauauagu uaucggaguc ggagaaaaga aaauaacuca ucauuggcau agaucaggua     1860

gcacgauagg aaaagcauuu gaagcaacag ucagaggugc caaaagaaug gcaguauugg     1920

gagauacagc augggacuuu ggaagugucg gaggagcacu aaauagcuua ggaaaaggaa     1980

uacaucaaau uuucggagca gcauuuaaau cuuuauuugg aggaaugagc ugguucucac     2040

aaauacuuau aggaacauug cugauguggu uaggacucaa uaccaaaaac ggaagcauau     2100

cauuaaugug ucuagcacug ggaggugucu uaauauuccu aucgacagca guaucagcag     2160

augugggaug uugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  6926
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6926
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcaagaaa agaaaaaaaa agacgaggag cagacacaag uguaggaaua guaggauuac      120

uuuugacaac ugcaauggca gcggaaguaa caagaagagg aagugcauau uauauguauu      180

uagaccgcaa ugaugccggu gaagcgauau cauucccaac uacuuuagga augaauaaau      240

guuauauaca aauaauggau uuaggacaua ugugugaugc aacgaugagc uaugaaugcc      300

ccauguuaga ugaaggagua gaaccagacg auguagacug uugguguaau acaacaagua      360

cauggguagu auauggaacg ugucaucaca aaaaaggaga agcaaggaga aguaggagag      420

caguaaccuu accaucccau agcacucgaa aauuacaaac aagaagccaa acaugguuag      480

aaagucgaga auauacaaaa cauuuaauaa gagucgaaaa uuggauauuu agaaauccag      540

gauucgcauu agcagcagcu gcaauagcau ggcuauuagg uucaucaaca agucaaaaag      600

uaauauauuu aguaaugauu uuacuaauag caccagcaua uucaauaaga uguauuggag      660

uaagcaaccg cgauuuugua gaaggaaugu caggaggaac auggguagac auaguacuag      720

aacauggagg gugugucaca guaauggcac aagauaaacc aacgguagau auagaauuag      780

uaacgaccac cguuucuaac auggcugaag uacgaucaua uugcuacgaa gcaucaauaa      840

gcgacauggc aucagauucu agauguccaa cucaaggaga agcguauuug gauaaacaau      900

ccgauacaca auacguaugc aagaggacuc uaguagauag ggguugggga aacggaugug      960

gguuauuugg aaaaggaagu uuaguuacau gugcuaaguu ugcguguagu aaaaaaauga     1020

caggaaaguc aauucaacca gaaaauuugg aauacagaau aauguuaagc guacauggcu     1080

cacaacacag cggaaugaua gugaaugaca caggacauga aacagaugaa aauagagcaa     1140

aaguagaaau aacaccaaau aguccaagag cagaagcgac acuaggaggu uuugguucau     1200

uaggucuaga uugcgaaccu cgaacagguu uagacuuuuc agaucuguau uauuuaacaa     1260

ugaacaauaa acauugguua guccauaaag aaugguuuca cgauauaccu uuaccguggc     1320

acgccggagc agacacagga acaccccauu ggaauaauaa agaagcacua guagaguuua     1380

aagacgcuca ugcaaaaaga caaacuguag uaguuuuggg uagccaagaa ggagccguac     1440

auacagcauu agcaggagcg cuagaagcag agauggacgg cgcuaaagga agauuauccu     1500

ccggacauuu aaaaugucga uuaaaaaugg auaaauuaag acuaaaagga guaucauaua     1560

guuuaugcac cgcagcuuuu acauuuacaa aaauaccugc agaaacucua cauggaaccg     1620

uaacaguaga aguucaauau gcaggcacag auggaccaug uaaaguuccu gcacaaaugg     1680

cuguagauau gcaaacacuc acacccguug gaagauuaau aacagcgaac ccaguaauaa     1740

cugaaagcac agaaaauagu aaaaugaugu uagaauuaga cccaccauuc ggagauaguu     1800

auauaguaau agguguagga gaaaaaaaaa uaacucacca uuggcacaga ucagggucaa     1860

cuauuggaaa agcauuugag gcaacaguaa gaggagcgaa acguauggca guauuaggag     1920

acaccgcgug ggacuuuggu agcguaggag gagcauuaaa cucguuagga aaaggaaucc     1980

aucagauauu cggagcagcc uuuaaaaguc ucuucggagg aaugagcugg uucucacaaa     2040

uuuuaauagg aacauuacua auguggcuag gauuaaauac aaaaaauggc aguauaucau     2100

uaaugugcuu agcucuagga ggaguacuaa uauucuuaag uacugcaguc ucagcugaug     2160

ugggaugcug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  6927
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6927
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

caagaaaaga aaaaaaacgu cguggugccg auacaagugu aggaauagua ggauuauugu      120

uaacaacagc aauggcagca gaaguaacac gaagaggauc agcauauuac auguauuugg      180

auagaaauga ugcgggagaa gcaauaucau uuccaacuac acuuggaaug aauaaauguu      240

auauucaaau uauggauuua ggacauaugu gcgacgcuac aaugucauac gaaugcccga      300

uguuagacga agggguggaa ccagaugacg ucgauuguug guguaauaca acgucaacgu      360

ggguaguaua uggaacaugc caucacaaaa aaggcgaagc uagaagaagu cguagagcag      420

uaacauuacc aagccauagu acaagaaaau uacaaacacg aucccaaacu ugguuagaau      480

caagggaaua cacuaaacau uugauaagag uagaaaauug gauauuuaga aauccggguu      540

ucgcccuugc ggcagcagca auagcauggu uacuaggauc uucaacaagu caaaaaguaa      600

uauauuuagu aaugauacuu uuaauagcac cagcuuauag cauaagaugc auugguguaa      660

guaauaggga cuuuguagaa gguaugucag guggaacaug gguagauaua guacuagaac      720

auggagguug cguaacaguu auggcacaag auaaaccaac ggucgacaua gaacuaguua      780

caacaacagu uagcaauaug gcagaaguaa gaucauauug uuacgaagca ucuauaagcg      840

acauggcaag ugauucuaga uguccaacac aaggagaggc auaccuugau aaacaaagug      900

auacucaaua uguaugcaaa agaacauuag uagaucgagg auggggaaac gggugugguc      960

uauuugguaa aggaucauua guaacgugug caaaauuugc auguucgaaa aaaaugacag     1020

gaaaaucaau acaaccagaa aacuuagaau auagaauaau gcucagcgua caugguucac     1080

aacauucagg aaugauugua aaugauacag gucaugaaac agaugaaaac cgagcaaaag     1140

uagaaauuac uccaaauagu ccaagagcug aagcaacacu uggagguuuu ggaagucuag     1200

gacuagacug cgaaccaaga acuggauuag auuuuucuga ucuauacuau cuaacaauga     1260

acaauaagca uugguuagua cauaaagagu gguuucauga uauuccacua ccuuggcaug     1320

caggugcuga uacaggaacc ccacauugga auaauaaaga agcuuuggua gaauuuaaag     1380

augcacaugc aaaaagacaa acuguaguag uauuaggauc acaagaagga gcaguacaca     1440

ccgcacuagc aggagcuuua gaagcugaaa uggauggagc caaaggaaga uuauccagug     1500

gacauuuaaa augcagauua aagauggaca aacuacgacu aaaaggagua agcuauucau     1560

uauguacagc agcauuuaca uuuaccaaaa uaccugcuga aacucuacac ggaacaguaa     1620

caguagaagu acaauaugcg gguacagaug gaccauguaa aguaccagcu caaauggcug     1680

uagauaugca gacuuuaaca ccgguaggaa gauugauaac ugcaaauccu guaauaacug     1740

aaucaacaga aaauaguaaa augauguuag aauuagaucc accauucgga gauucauaca     1800

uaguaauugg ugucggggaa aaaaaaauaa cccaucauug gcauagauca ggaaguacaa     1860

uaggaaaagc uuuugaagca acaguaagag gagcaaaaag aauggcggug cucggagaua     1920

cagcuuggga uuuuggcagu guagguggug cacuaaauuc auuagguaaa ggaauacauc     1980

aaauuuuugg agcugcguuu aaaucacuau ucggaggaau gucuugguuu ucucaaauau     2040

uaauugggac uuuguuaaug ugguuagguc uaaauacaaa aaauggguca auaucacuaa     2100

uguguuuagc acugggagga guauuaauuu uuuuaaguac agcaguaagc gcagacguag     2160

gauguugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6928
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6928
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaagaaaa aaaaagaaga ggagcugaua caucaguagg aauaguagga cuauuguuga      120

caacagcaau ggcagcagag guaacaagac gagguagcgc auauuacaug uauuuagaua      180

gaaacgaugc aggagaagcu auaucauucc caacuacauu aggaaugaac aaaugcuaua      240

uacaaauuau ggauuuagga cauaugugug augcaacaau gaguuaugaa ugcccuaugu      300

uagaugaagg aguagaaccc gacgauguug acugcuggug caauacgaca aguacauggg      360

uuguauaugg uacaugucau cauaaaaaag gagaagcaag acgaaguaga cgcgcaguca      420

cauuaccgag ccauagcacg cguaaacuac aaacacgauc acaaacuugg cuagagagua      480

gagaauauac uaaacauuua aucagagucg aaaacuggau auucaggaau cccggauuug      540

cauuagcagc agcagcaauc gcauggcuau uaggcucaag uacuagucaa aaaguaauau      600

auuuaguaau gauauuacua auugccccag cauacaguau aagauguaua gguguaagca      660

accgagacuu cguagaagga augucuggag gaacaugggu ugacauugua cuagaacacg      720

gagguugugu cacagugaug gcgcaagaua aaccuacagu ugauauugaa cuaguuacaa      780

caacaguaag uaacauggcu gaaguaagau cauacuguua ugaagcaucu auaagugaua      840

uggcuaguga cagucgaugu ccaacacaag gcgaagcaua cuuagauaaa caaucagaua      900

cucaauaugu auguaaaaga acacuagucg acagagggug gggaaacgga uguggacuau      960

uuggaaaagg aucgcuagug acaugugcaa aauuugcaug cucaaaaaaa augacaggca     1020

aaucaauaca accugaaaau uuagaauacc ggaucauguu aucaguccau gguucacaac     1080

auagcggaau gauagucaau gauacaggac augaaacgga ugaaaacagg gcaaaaguag     1140

aaauaacucc aaacagucca agagcagaag ccacauuggg aggcuuugga agcuuaggau     1200

uagacugcga accgcguaca ggcuuagauu ucuccgaucu uuauuaucua acuaugaaua     1260

auaaacauug guugguacau aaagaauggu uucacgacau cccauuaccu uggcaugcag     1320

gagcagauac aggaacacca cauuggaaca auaaggaagc auuaguagaa uucaaagacg     1380

cacaugcaaa aagacaaaca guaguaguac uaggaucaca agaaggagca guccauacug     1440

cucucgcagg agcguuagaa gcagaaaugg acggagcaaa agggcguuug agcucaggac     1500

auuuaaaaug cagauuaaaa auggauaaau uacgauuaaa aggaguaucu uacucgcuau     1560

guaccgcagc uuucacauuc acaaaaauac cagcagaaac cuuacauggg acaguaacgg     1620

uugaagugca auaugcaggg acagauggcc cauguaaagu acccgcucaa auggcaguug     1680

acaugcaaac ucuaacacca guaggaagau uaauuacagc aaacccagua auuacagaaa     1740

guacugaaaa caguaaaaug auguuggaac uagauccacc auuuggagac agcuauauag     1800

uuaucggcgu aggugaaaaa aaaauaacac aucauuggca uagaaguggu aguacgauag     1860

gaaaagcuuu cgaagcuacg guaagaggag caaaaagaau ggcagucuug ggagauaccg     1920

caugggauuu ugguaguguu gguggugcau uaaauucauu agguaaagga auacaccaaa     1980

uauuuggagc agcauucaaa ucuuuauucg gagguaugag uugguuuuca caaauauuga     2040

uaggaacauu acuaaugugg cuaggcuuaa auacaaaaaa ugguuccaua aguuuaaugu     2100

guuuagcauu agggggugug uuaauauuuu uaucuacugc aguuagugcu gauguaggcu     2160

guugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6929
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6929
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aagaaaagaa aagaagaggu gcagacacuu caguaggaau ugugggauug cuacuuacaa      120

cugcaauggc agcagaagua acacgacgug gaucugcaua cuauauguac uuagacagga      180

augacgcugg ugaagcaaua aguuuuccaa caacauuagg caugaacaaa ugcuacauac      240

aaauaaugga ccuaggacau augugugacg caaccaugag uuacgaaugu ccaauguuag      300

augaaggagu agagcccgau gauguagauu gcugguguaa uacaacaagc acauggguag      360

uguauggaac augucaccau aagaaaggug aagcaagaag aucaaggaga gcaguaacuc      420

uuccaucaca uucaacgcga aaauuacaaa caaggucaca aacaugguua gaaaguagag      480

aauauacgaa acaccucaua agaguagaaa auuggauauu cagaaauccc ggcuuugcac      540

uagcagcagc agcaauagca ugguuacugg gaaguaguac aucacagaaa guaauauauu      600

ugguaaugau auuacuuaua gccccagcau auucaauuag auguauagga guuucgaaua      660

gagauuucgu ggaaggcaug aguggaggaa cguggguaga cauaguuuua gaacauggag      720

gaugugucac agugauggca caagauaaac cgacaguaga uauagaauua gugacaacua      780

caguaaguaa uauggcagaa guuagaaguu auuguuauga agcauccaua agcgauaugg      840

cuucagauuc aagauguccg acucaaggag aagcauauuu agauaaacaa agugacacac      900

aauacguaug uaaaaggacu cuaguagaua gaggaugggg uaauggaugu ggguuauuug      960

guaaaggauc uuuagucaca ugugcaaaau ucgcaugcag uaagaaaaug acaggcaaaa     1020

guauccagcc agaaaauuug gaauauagga uaaugcuauc aguacacggu ucucaacauu     1080

ccggaaugau ugucaaugau acaggucaug aaacagauga aaacagggcg aaaguggaga     1140

uuacaccaaa cucaccaaga gcggaagcaa cacuaggugg auuugguuca uugggauuag     1200

auugcgaacc aagaacagga uuagauuuuu cagaucugua cuauuuaacu augaacaaca     1260

aacauugguu aguacauaaa gaaugguuuc augauauacc auuaccaugg caugcgggag     1320

cagauacagg aacgccacac uggaacaaca aagaagcacu cguagaauuu aaagacgcac     1380

augcgaaacg acaaacagua guaguauuag gaagccagga aggagcagua cauacagcac     1440

uagcaggcgc auuagaagca gaaauggaug gagcaaaagg uagauuaagu aguggacauu     1500

uaaaauguag guuaaaaaug gauaaacuaa gauuaaaagg aguaaguuau aguuuaugca     1560

cugccgcauu cacauuuacc aaaauaccug cagagacauu acauggcaca guaacaguug     1620

aaguacaaua cgcaggcaca gaugggccau gcaaagugcc cgcucaaaug gcaguagaua     1680

ugcaaaccuu aacgccaguu ggcagauuaa uaacugcaaa cccaguuaua acugaaucga     1740

cagaaaacag caaaaugaug uuagaacuag auccgccuuu uggagauuca uauauaguaa     1800

uuggaguagg agaaaagaaa auaacacauc acuggcauag aaguggaagc acaauaggaa     1860

aagcauuuga agcgacagua aggggagcga aaagaauggc cguauuagga gauacagcau     1920

gggacuucgg uaguguagga ggagcuuuaa auagucuagg aaaaggaaua caucaaauau     1980

uuggagcagc uuuuaaauca cuauuuggug guaugucaug guuuucacaa auauuaauag     2040

gaacauuacu aauguggcua ggccuaaaua ccaaaaaugg aaguauaagu uuaauguguu     2100

uagcauuagg ugguguauua auauuucuaa guaccgcagu aucagcagau guuggauguu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6930
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6930
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaag       60

aaaagaaaag aagaggugca gauacauccg uaggcauagu agguuuguua uuaacaacag      120

caauggcugc cgaagugacu agacguggaa gugcauacua cauguauuua gauagaaaug      180

augcuggaga agcuauuuca uuuccuacca cauuaggaau gaauaaaugu uacauacaaa      240

uaauggaucu aggucacaug ugcgaugcca caaugagcua cgaaugucca auguuagaug      300

agggaguaga accagacgau guugauuguu gguguaauac aacaucaacg uggguaguuu      360

augguacaug ccaucauaag aaaggagaag cuagaagauc aagacgagca guaacauuac      420

caagucacuc aacuagaaaa cuacaaacaa gaagccaaac auggcuagaa agcagagaau      480

auacuaaaca ccuuauuaga guagaaaauu ggauauuuag gaacccagga uuugcuuuag      540

cggcugcugc aauugcaugg cugcuuggau caaguacauc ucagaaaguc auauaucugg      600

uaaugaucuu auuaauagcc ccugcauaca guaucaggug cauaggagua ucgaaucgug      660

auuucguaga aggaaugagc gggggaacau ggguugacau aguauuagag cauggaggau      720

guguuacagu aauggcacaa gauaaaccaa cugucgauau agaauuagua accaccacag      780

uaagcaacau ggcagaagua agaaguuauu guuaugaagc aucaauaagc gauauggcau      840

cugacucacg auguccaaca caaggggaag cauaucucga uaaacaguca gauacucaau      900

auguuuguaa aagaacucua guagaucgag gcuggggaaa cggaugcggu cucuucggaa      960

aaggaucauu aguaacaugu gcaaaguucg cauguaguaa gaaaaugaca ggaaaaucca     1020

uacaaccaga aaauuuagaa uauagaauua uguuaagcgu acaugguucu caacacucag     1080

gaaugauagu aaaugauaca ggccaugaaa cagacgaaaa cagagccaaa guagagauaa     1140

caccaaacag uccacgagcu gaagcuacau uaggaggguu cggaucauua ggacuggauu     1200

gugaacccag aacuggucua gacuuuucag accuuuacua uuuaacaaug aauaauaaac     1260

auuggcuagu acacaaagaa ugguuucacg acauuccguu accauggcau gcgggugcag     1320

auacaggcac accacauugg aauaauaaag aagcucuagu agaguuuaaa gaugcacaug     1380

caaaacgaca aacagucgua guacuaggua gucaagaagg agcaguacau acugcauuag     1440

cuggagcauu agaagcagaa auggauggag caaaaggaag gcuaucaucc ggucaucuaa     1500

aauguagacu aaaaauggau aaacugagau uaaagggagu cagcuauuca uuauguacag     1560

cagcauucac auucaccaaa aucccagcag agacuuuaca uggaacagua acagucgaag     1620

uacaauaugc agguacagau ggaccaugua aaguuccagc acaaauggcu guagauaugc     1680

aaacguuaac acccguuggu agacuaauaa cagcuaaccc aguuauuaca gaaagcacag     1740

aaaauucaaa aaugauguua gagcuagacc caccauucgg ugauagcuau auugucauag     1800

gagucggaga aaagaaaaua acacaccauu ggcacagauc aggaucaaca auaggaaaag     1860

ccuuugaagc uacaguaaga ggagcaaaac gaauggccgu auuaggugau acagcauggg     1920

auuuuggauc aguuggagga gcacuuaacu cucuagguaa aggaauacau caaauauuug     1980

gagcagcuuu caaaucucua uuugguggua ugaguugguu uagucaaaua uuaauuggua     2040

cauuacuaau gugguuaggu cuaaauacua aaaaugguuc aauaaguuua auguguuuag     2100

cauuaggagg cguuuuaaua uuuuuaagua cagcaguaag cgcugacguu ggcuguugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6931
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6931
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaaa       60

agaaaagaag aggagcagau acaaguguag gaauuguugg acuauuauua acuacagcca      120

uggcagcaga aguaacgaga agggguagug cuuauuacau guaucuagac aggaaugaug      180

caggggaagc aauaucuuuc ccaacuacau uaggcaugaa uaaauguuau auucaaauaa      240

uggaucuagg acauaugugu gaugcaacaa ugucauauga augcccaaug uuagaugagg      300

guguggaacc cgaugaugua gauugcuggu guaauacaac aagcacaugg guuguauaug      360

gaaccugcca ucauaagaaa ggagaagcaa gaagaucuag gagagcagug acguuaccaa      420

gucauuccac aagaaaauua caaacaagau cgcagacaug guuagaaagc agagaauaca      480

caaaacacuu aauaagagua gaaaauugga uuuucagaaa uccagguuuu gcauuagcag      540

ccgcagcaau agcauggcuc cuaggaucaa guacaucuca aaaaguuaua uauuuaguca      600

ugauacuacu aauugcuccu gcauauagua uaagaugcau agguguuagu aauagagauu      660

uuguagaagg gaugucagga gguacauggg uagauauagu auuagaacau ggaggaugug      720

uaacuguaau ggcacaagac aaaccuacag uagauauaga acucguaacu acaaccguaa      780

guaauauggc agagguaaga ucauauuguu acgaagcauc aauaucagau auggcaucag      840

auaguagaug cccuacgcaa ggagaagcau aucuagauaa acaaagcgau acucaauaug      900

uauguaaacg aacuuuagua gauagagggu ggggaaaugg auguggacua uuugguaaag      960

gaucuuuagu aacuugugca aaauuugcuu guucaaagaa aaugacugga aaaucaauuc     1020

aaccagaaaa uuuagaauau cgaauaaugu uaucaguaca uggaucgcaa cacagcggaa     1080

ugauaguaaa cgauaccgga cacgaaacug acgaaaacag agcaaaagua gagauaacac     1140

ccaauagucc aagagcagaa gcuacacuag gaggauuugg cagccuugga cucgacugug     1200

agccaagaac gggauuagac uuuagcgauc uauauuacuu aacaaugaau aacaaacauu     1260

gguuagucca uaaagaaugg uuucaugaca uuccacuacc auggcacgcu ggugcagaua     1320

cuggaacacc gcacuggaau aauaaagaag cccuaguuga auuuaaagau gcacaugcaa     1380

aaagacaaac agucguagua uuagguucuc aggaaggagc aguucauaca gcauuagccg     1440

gugcauuaga agcagaaaug gauggagcaa aaggaagauu aaguucugga caucugaaau     1500

gucgacuaaa aauggacaaa cuaagauuaa aaggaguauc auacucauua uguacagcag     1560

cauuuacauu cacuaaaaua ccagcagaaa cauugcacgg uacaguaaca guagaagugc     1620

aauaugcugg aacugauggc ccguguaaag uaccggcaca aauggcugua gauaugcaga     1680

cauuaacacc aguaggaaga uuaauaacag caaauccugu aauaacagaa agcacagaaa     1740

acaguaagau gauguuagaa uuagacccac ccuucgggga cagcuauaua guuauaggag     1800

uuggagaaaa gaaaauaaca caucacuggc auaggucagg aucaacaaua gggaaagcau     1860

ucgaagcgac uguuagagga gcaaaaagaa uggcgguauu gggagauacu gcaugggauu     1920

ucggaucagu aggaggagca uugaauaguc uaggcaaagg uauacaccaa auauucgggg     1980

cagcauuuaa auccuuauuu gguggaauga guugguuuag ucaaauuuua auaggaacuu     2040

uacugaugug guuaggacua aauacuaaaa auggauccau cucauuaaug ugucucgcgc     2100

uaggaggagu acuaauuuuc uuaucuacag caguaucagc ggacguagga ugcugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6932
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6932
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

aaagaagggg agcugauaca agcgucggaa uaguaggacu auuauuaaca acagcaaugg      120

cagcagaagu aacacgaaga ggcagcgcuu auuauaugua uuuagauaga aaugaugcag      180

gugaagccau aucauuucca acaacucuag gaaugaauaa auguuacaua caaauaaugg      240

auuuaggaca uaugugugau gcaacaauga guuaugaaug cccaaugcug gaugaaggug      300

uugaaccuga ugauguagau uguuggugua auacaacauc aacaugggua guauacggaa      360

caugccauca caagaaagga gaagcaagaa gaucaagaag agcaguaaca cuaccaaguc      420

acucuacaag aaaauuacaa acaagaaguc aaacgugguu agaaucaaga gaauauacaa      480

aacacuugau acgaguagaa aacuggaucu uuagaaaucc aggauucgcc cuugcagccg      540

cugcaauugc augguuacua gguaguagua cuagucaaaa aguuauauac cuugucauga      600

uauuauuaau agcuccagca uacucaauaa gaugcauagg cguaaguaau agagauuuug      660

ucgaaggaau guccggagga acauggguug acauaguauu agaacacgga ggauguguaa      720

caguaauggc acaagauaaa ccaacuguug auauugaguu aguuacaacu acaguaucaa      780

auauggcaga gguacgaagu uauuguuacg aggcaaguau aucugauaug gcaucugauu      840

cuagaugucc aacucaagga gaagcauauc uugacaaaca aucugauaca caauaugucu      900

guaaaagaac auuaguagau cguggauggg ggaacggaug uggauuauuc ggaaaaggau      960

cacuaguaac cugugcuaaa uuugcauguu ccaaaaaaau gacugguaaa ucaauccaac     1020

cagaaaauuu agaauacagg auaauguuau caguccacgg uagucaacau agcgguauga     1080

uaguuaauga uacaggacau gaaacagaug aaaaucgagc aaaaguugaa auaaccccaa     1140

auucaccaag agcagaagcc acacuagggg gcuuuggauc auugggauua gacugcgaac     1200

cucgaacagg auuggacuuc ucugauuuau auuaucuaac gaugaacaau aaacacuggu     1260

uaguacacaa agaaugguuu cacgauauac cucuaccaug gcaugcaggu gcagacacag     1320

gaacaccaca uuggaacaau aaagaggcac uaguagaauu caaagaugcg cacgccaaac     1380

gacaaacagu cguaguuuua gguucacaag aaggagcugu acauacagcu uuagcagggg     1440

cguuagaagc agaaauggau ggugcgaaag gacguuuguc aucuggucau uuaaaauguc     1500

gauugaaaau ggauaaacua cgacuaaaag gaguaagcua cucacuuugu acugcggcuu     1560

uuacauuuac aaaaauacca gcagaaaccc ugcauggaac uguuacugua gaaguacaau     1620

augcuggcac agacggaccu uguaaaguuc cagcccaaau ggcaguagau augcaaacac     1680

uaacuccagu aggaagacuu auaacagcaa auccagugau uacagaauca acagaaaaua     1740

guaaaaugau guuagaacuc gauccaccau uuggagauag cuacauaguc auaggaguag     1800

gcgaaaaaaa aauaacacac cauuggcaua gaagcggaag uaccauagga aaggccuuug     1860

aagcgacagu aagaggagcu aaacgaaugg caguacuagg ggauacggca ugggacuuug     1920

guagcguagg aggagcauua aacucucucg gaaaaggaau acaccaaauc uuuggagcug     1980

cauuuaaauc auuauuuggu ggcaugaguu gguuuagcca aauccuaaua ggaacauuau     2040

uaaugugguu agguuuaaac acaaaaaaug gauccauuuc auugaugugu cuggcacuug     2100

gaggaguauu aaucuuucuu uccacugcag uaagcgcaga uguaggaugu ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6933
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6933
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

gaagaggagc agauacuuca guaggaauag uaggacuauu auuaacaaca gcaauggccg      120

cagaagucac aagaagagga ucagcauacu auauguaccu agauagaaac gaugcuggag      180

aagcaauauc guucccaaca acacuaggaa ugaauaaaug uuauauucag auaauggauc      240

ucggacauau gugcgaugca acaaugucau augaaugucc aauguuagau gaaggagucg      300

aaccugauga uguagauugu ugguguaaua caacaucaac auggguagua uauggaacau      360

gucaucacaa aaagggcgag gcuagaagau cccguagagc cgucacauua ccaucgcaua      420

guacaagaaa gcuacaaaca cguucucaga cauggcuaga aaguagagaa uauacaaaac      480

auuuaauccg agucgaaaac uggauauucc gcaauccagg guuugcacua gcugccgcag      540

cuauugcaug gcugcuaggu ucaucaacgu cacaaaaagu uauauaccua gugaugauau      600

uauuaaucgc accagcauau aguauuagau guauaggugu aaguaauaga gacuuuguag      660

aagguaugag cggaggaaca ugggucgaua uaguauuaga acauggcgga ugugugaccg      720

uaauggcaca agauaaaccc acgguagaua uagaauuggu cacaacuacu guaucaaaua      780

uggcagaagu aagaucauac ugcuacgaag caucaaucuc agauauggcc ucagauucca      840

gauguccgac ccaaggagaa gcauaucuag acaaacaauc ggauacacaa uauguaugua      900

aacgaacgcu aguugauaga ggauggggaa auggaugugg auuauuugga aaaggaaguu      960

uagucacaug ugcaaaauuc gcaugcagca agaaaaugac cggaaaaucc auacaaccag     1020

aaaaucuaga auauagaaua augcuaucag uacacggaag ccagcauagu ggaaugauug     1080

uaaaugauac cggacaugaa acugacgaaa auagagcaaa aguagaaaua acaccaaaua     1140

guccaagagc agaagcaaca uuagggggau ucggaucacu agguuuagac ugcgaaccac     1200

gaaccggauu agauuucagu gacuuauacu auuuaacaau gaauaauaaa cauugguuag     1260

uacacaaaga augguuucau gacauaccau uaccauggca ugcaggagca gacacaggaa     1320

caccacacug gaacaauaaa gaagcauuag uagaauuuaa agaugcacau gcaaaacggc     1380

aaacaguagu aguauuagga agucaagaag gcgcuguaca cacagcacua gcuggagcau     1440

uggaagcaga aauggacgga gcaaaaggaa gguuaucaag uggacacuua aaauguagau     1500

uaaagaugga uaaacuaaga cuaaaaggag uaaguuauuc auuaugcaca gcugcuuuua     1560

cauuuacuaa aaucccagca gaaacacugc acgggacagu aacaguugaa guacaauaug     1620

cagguacuga uggaccaugu aaaguaccug cucaaauggc aguugauaug caaacauuaa     1680

caccaguagg gagauuaauu acugcaaacc caguaauaac agaaucuacu gaaaauucaa     1740

aaaugauguu agaauuagau ccaccguuug gagauaguua uauaguaaua ggaguuggag     1800

agaaaaagau aacacaucau uggcacagaa gcggaucaac uauaggaaaa gcauuugaag     1860

ccacaguaag aggagcaaaa agaauggcag uguuaggaga uacagcaugg gauuuuggau     1920

cagugggagg ugcacuuaau ucauuaggaa aagguauuca ccaaauauuc ggagcagccu     1980

uuaaaagucu uuucgggggu augaguuggu ucagccaaau acuaauagga acacuacuua     2040

ugugguuagg auuaaauacu aaaaauggua guauaucuuu aauguguuua gcauuaggcg     2100

gaguacugau auuuuuauca acagcgguua gcgcugacgu ggguugcuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6934
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6934
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaggc       60

guggcgcaga uacaucagua gggauuguag gauuauuauu aacaacagca auggcagcag      120

aaguuacaag aagaggaagu gcauacuaca uguauuuaga uagaaacgau gccggagaag      180

caauauccuu uccaacaaca uuaggaauga auaaauguua uauacaaaua auggacuuag      240

gacacaugug ugacgcaacu augucauaug aaugcccaau gcuagaugaa ggagucgaac      300

cugaugacgu agauugcugg uguaauacaa caaguacaug gguaguauau ggaacauguc      360

aucauaagaa aggcgaagca agacgaucaa ggagagcagu uacacugccc ucacacucaa      420

caagaaaacu acaaacaaga ucacaaacuu gguuagaauc uagagaauau acaaaacauc      480

uuauaagagu cgaaaauugg auauuuagaa acccaggauu ugcauuagcc gcagcagcaa      540

uagcaugguu auuagggagu ucaacaucac agaaaguuau auauuuagua augauauuau      600

uaauugcacc agcauacuca auaagaugua uaggaguuuc gaauagggau uucguagaag      660

gaaugucggg agguaccugg guagauauag uuuuagaaca uggcgguugu guaacaguua      720

uggcacaaga uaaaccuaca guagauauag aacuuguaac aacaacaguu agcaacaugg      780

cugaaguaag aagcuauugu uacgaagcau caauaucaga cauggcaucc gauucaagau      840

gcccgacaca aggagaagca uaucuagaua aacaaucaga uacacaauau guaugcaaac      900

gaacucuagu agacagaggc uggggaaacg guugcggacu auucggaaaa gguagccuag      960

uaaccugugc uaaauucgca uguucaaaga aaaugacugg aaaaucaauu caaccugaaa     1020

acuuagaaua uagaauuaug uuaagcgugc auggaagcca acauucaggu augauuguca     1080

augauacggg acaugaaaca gaugaaaacc gugcaaaagu agaaauaaca ccaaauuccc     1140

cgagggcaga agcaacacua gggggauuug gaucacuagg cuuagauugu gaaccuagaa     1200

cuggauuaga uuuuagugau cuauauuacc uaacaaugaa caauaaacau ugguuaguuc     1260

auaaagaaug guuucaugau auuccauuac cguggcacgc cggugcagau acagguacgc     1320

cacacuggaa uaacaaagaa gcauuaguag aauuuaaaga ugcacaugcu aaacgucaaa     1380

cugucguagu auuagguuca caagaaggag caguacauac agcauuagca ggagcccuag     1440

aagcggaaau ggauggagca aaaggacgcc uaucaagugg acacuuaaaa ugucgcuuaa     1500

aaauggacaa gcuaagauua aaaggaguca guuauucucu augcacagcc gcuuucacau     1560

ucacgaaaau cccugcggaa acacuucacg gaacaguaac uguagaaguu caauacgcag     1620

guacagacgg cccguguaaa guaccagccc aaauggccgu agauaugcaa acauuaacac     1680

caguaggaag acuaauaacu gcaaauccag uuauaacaga aucgacagaa aacagcaaaa     1740

ugauguuaga acuagaccca ccauuuggag auucuuacau cguuaucgga guaggagaaa     1800

agaaaauuac acaccacugg cauagaagug gaucaacaau uggaaaagca uucgaagcaa     1860

cgguaagagg agccaaacga auggcaguuu uaggagacac ugcaugggac uucggaagcg     1920

uaggaggugc auuaaacucg uuagggaaag gcauucauca gauauuugga gcagcauuca     1980

aaucauuauu cggagguaug ucaugguuua gucaaauacu aauuggaacu uuacuaaugu     2040

gguuggguuu aaacacuaaa aacgguagua uaucacuuau guguuuagcc uuaggcggag     2100

uguuaauauu uuuaucaaca gcaguaagcg cugauguagg uuguugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6935
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6935
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gagcugauac aucaguagga auuguagguu uauuacuaac aacagcaaug gcagcagaag      120

uuacaagaag aggaucagcc uauuauaugu auuuagaucg aaacgaugcc ggagaagcaa      180

uaaguuuucc aacaacuuua ggaaugaaua aaugcuauau acaaaucaug gaucuuggac      240

acauguguga cgcaacaaug uccuacgaau guccaauguu agacgaagga guagaaccug      300

acgacgucga uuguuggugu aauaccacau caacaugggu aguuuacggu acaugccauc      360

auaagaaagg agaagcaagg cgaucaagga gagcaguaac auuacccuca cauaguacaa      420

gaaaauuaca gacuagauca caaacauggc uugaaucgag agaauacacu aaacaccuaa      480

uaagaguaga aaacuggauu uuuagaaauc caggauuugc cuuagcagca gcagcgauag      540

cauggcuacu ugggucuagu acaagucaaa aaguaauuua cuuaguaaug aucuuacuaa      600

uugcaccagc auacucaaua agaugcauug gaguaucaaa uagagacuuu gucgaaggua      660

ugagcggugg aacuugggua gacauugucu uggagcacgg uggaugcgua acuguaaugg      720

cacaagacaa accaacagug gacauagaau ugguaacaac aaccguaagu aauauggcag      780

aaguuagauc cuauugcuac gaggcaucaa uaagugacau ggcaagugau ucaagauguc      840

caacacaagg agaggcuuau cucgauaaac aaagugauac ccaauaugua uguaaaagaa      900

cauuaguuga cagagguugg ggaaauggcu guggacuauu cgguaaaggg agcuuaguaa      960

caugugcaaa auuugcaugu ucaaagaaaa ugacaggaaa aucuauacaa ccagaaaauc     1020

uagaauauag gauuauguua agcguucaug gaucgcaaca uaguggaaug auugucaaug     1080

acacaggaca ugaaacugac gaaaaccgag cuaaagugga aauaacacca aauucaccac     1140

gagcagaagc uacacucgga ggcuucggau cacuaggauu ggacugugaa ccaagaacag     1200

guuuagauuu cagcgaucua uauuauuuaa cuaugaauaa uaaacauugg uuagugcaua     1260

aagaaugguu ucaugauaua ccauuaccuu ggcacgcagg agcagauaca ggcacaccac     1320

acuggaauaa uaaagaagcc cuaguagaau uuaaagaugc acacgcuaaa agacaaacag     1380

uaguuguacu aggaucacaa gaaggugcug uacauacagc acuugccggg gcauuagaag     1440

cagaaaugga uggagcuaaa ggaagauuaa gcucagguca cuuaaaaugu agauuaaaaa     1500

uggauaaauu aaggcuaaaa ggaguaagcu acucuuuaug uaccgcagcu uucacauuua     1560

caaaaauacc agcagaaaca uuacauggaa caguaacugu agaaguacaa uaugcaggua     1620

cagauggucc auguaaagua ccagcacaga uggcaguaga uaugcaaaca uuaacaccag     1680

uagggagauu gauaacagcu aauccuguca uaacagaauc aacugaaaau agcaaaauga     1740

ugcuagaacu agauccacca uucggugauu cauauauagu aauaggaguu ggagaaaaga     1800

aaauaacgca ucacuggcau agaagcggua guacaauagg aaaagcauuu gaggcaacag     1860

uaaggggcgc aaaaagaaug gcuguauuag gagauacagc augggauuuc ggaagcgucg     1920

guggagcacu aaauucacua ggaaaaggua uucaucaaau auucggagca gcuuuuaaau     1980

cuuuguuugg aggaaugagu ugguuuucuc aaauacuaau uggaacauug cugauguggu     2040

uaggacuaaa cacuaaaaac ggaucaauua gcuuaaugug ucuagcucuc ggagguguau     2100

ugauauuuuu aucaacagcu guaucagcgg acguuggaug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6936
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6936
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggag       60

cugauacaag ugucggaaua gucggacuau uacuaacuac agcuauggca gcggaaguua      120

ccagaagagg cagcgcauau uauauguauu uagauagaaa ugaugcagga gaagcaauau      180

ccuuuccuac cacucuagga augaauaaau guuauauaca aauaauggac cuaggacaca      240

ugugcgaugc cacuaugagu uacgaaugcc cuaugcuuga ugaaggaguc gaaccugaug      300

auguagauug cugguguaau acaacaagua cauggguagu auacggaaca ugccaucaua      360

agaaaggaga ggccagaaga ucaaggagag caguaacacu acccucucau ucaacaagaa      420

aauuacaaac aagaagccaa acaugguugg aaaguagaga auauacaaaa caucuaauuc      480

gaguagaaaa uuggauauuu aggaacccag gauuugcacu agcagcagca gcaauugcuu      540

gguuacuagg aucaucaaca ucacaaaaag uaauauaccu aguaaugaua cuauuaauag      600

caccagcaua uaguauaaga uguauaggag uaucuaaccg agauuuuguu gaaggaauga      660

gcgggggaac auggguagau auaguguuag aacauggagg auguguuacc guaauggcac      720

aagauaaacc cacaguggac auagagcuag uuacaacuac cgucucaaau auggcagagg      780

uacgaagcua uugcuaugaa gcgagcauaa gugauauggc aagugauagc cgauguccua      840

cccaaggaga agcauaucua gauaaacaau cagacacuca auauguaugc aaacgaacau      900

uaguugacag aggauggggg aauggaugug gacuauuugg gaaaggaucu cuaguaacau      960

gugcaaaauu ugcuuguagc aagaaaauga cagguaaauc aauacaacca gaaaauuuag     1020

aguaucgaau uaugcuaagu gugcauggau cacaacauuc ggguaugaua guaaaugaca     1080

cuggacauga aacugacgaa aauagagcga aagucgaaau aacaccaaau uccccaagag     1140

cggaagcuac ucuaggaggc uucggaaguc uggguuuaga uugugaaccu agaaccggac     1200

uagauuucuc ugauuuauau uauuuaacaa ugaauaauaa acauugguua guacauaaag     1260

agugguuuca ugauauucca cuaccauggc augcuggugc ggauacagga acuccacauu     1320

ggaacaauaa agaagcauua guugaauuua aagacgcaca ugcuaaacga caaacagucg     1380

uaguacuagg aagucaagaa ggagcuguuc acacagcucu agcaggagca uuagaagcag     1440

aaauggaugg agcaaaagga agauuaucaa guggacaucu aaaauguaga uuaaaaaugg     1500

acaaacuuag auuaaaagga guaucuuauu cucuauguac agcugccuuu acauuuacaa     1560

aaauaccugc ugagacauua cacggaacag uuacaguuga aguucaauau gcaggcacug     1620

auggaccaug uaagguccca gcucaaaugg caguagauau gcaaacauua acaccaguug     1680

gaagauuaau aaccgcaaau ccuguaauaa cagaaagcac agaaaauuca aaaaugaugu     1740

uagagcuaga uccaccauuu ggagacucau acauaguaau aggaguaggu gaaaagaaaa     1800

uuacacauca uuggcauaga ucaggaagua caauagguaa agcauuugaa gcaacaguaa     1860

gaggagcaaa aagaauggca guccuuggcg auacugcaug ggauuuugga ucaguuggag     1920

gagcacuaaa cucucuaggc aagggaauac aucaaauauu uggagcagcu uucaaaaguu     1980

uauuuggagg aaugucaugg uuuucucaaa uacuaauagg aacauuacuu augugguuag     2040

gacuaaauac uaaaaacggc aguauaucuc uaauguguuu agcauuagga gguguuuuaa     2100

uauuucuuuc uacagccgua ucggcagaug uuggauguug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6937
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6937
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcug       60

acacaucagu uggaauagua ggacuauuau uaacaaccgc aauggcagca gaaguaacca      120

gaagaggauc agccuauuau auguaucucg auaggaacga cgcaggcgaa gcaauaucau      180

uuccuacaac acuagguaug aauaaauguu auauucaaau aauggaucug ggacauaugu      240

gcgacgcaac cauguccuau gaauguccua uguuagauga agguguagag ccagaugaug      300

uugacuguug guguaauaca acuaguacuu ggguaguaua cggaacaugu caucauaaaa      360

aaggagaggc ccgaagauca aggagagccg uaaccuuacc aagucauuca acaagaaaau      420

uacaaacuag gagucagacu uggcuagaaa gucgagaaua uaccaaacau uuaauacgag      480

uagaaaauug gauuuuucga aauccaggau uugcacuugc ugcugcugcg auugccuggu      540

uacuuggaag cucaacaagu caaaaaguaa uuuauuuagu uaugauauua uuaauagcac      600

cagcuuacag cauuagaugu auaggagugu caaauagaga cuuuguagag ggcaugagug      660

gagguacgug gguagauaua guauuagaac auggaggaug cgugacagua auggcacaag      720

acaaaccaac gguagauaua gaauuaguaa caacuaccgu aucgaauaug gcagaaguac      780

gaucauauug cuaugaagca ucaauaagug auauggcuuc cgacagcaga ugcccuacuc      840

aaggagaagc auacuuagau aagcaaagug auacccaaua uguuugcaaa cgcacacuag      900

uagauagagg augggguaau ggaugugguu uauuuggcaa aggaagucug guaacaugug      960

cuaaauuugc augcucaaaa aaaaugacag gaaaaucaau acaacccgaa aauuuagaau     1020

auagaauaau guuaagugug caugguucac aacauagcgg aaugauugua aaugauacug     1080

gacaugaaac agaugaaaau agagcaaaag uagaaauaac accaaacagu ccaagggcgg     1140

aagccacacu agguggauuu gguucauugg gacuagacug ugaaccgcga acuggcuuag     1200

auuuuagcga uuuauauuau uuaacaauga auaacaaaca uugguuagua cacaaagaau     1260

gguuccauga cauuccacuu ccauggcacg cuggcgcaga uacuggaaca ccucacugga     1320

auaacaaaga agcacuagug gaauucaagg acgcacacgc aaaaagacaa acaguaguag     1380

uucuaggauc acaagaagga gcaguacaua cagcuuuagc gggagcauua gaagcagaaa     1440

uggauggagc aaaaggaaga cuaucaagcg gacaucuaaa auguagguua aaaauggaca     1500

aacuaagauu aaaaggagug ucauauaguu uauguacagc agcauucacu uuuacaaaaa     1560

uuccagcuga aacauuacau gguacaguaa caguugaagu ucaauaugcu ggaacagaug     1620

gaccauguaa aguaccagca caaauggcag uagacaugca aacacuaaca cccguuggcc     1680

ggcuaaucac agcaaaucca guaauuacag aaucaacuga aaauucaaaa augauguuag     1740

aauuagaucc acccuuugga gauaguuaca uaguaauagg cguaggagaa aaaaaaauua     1800

cacaucauug gcauagaucu gggucaacaa uuggaaaagc guuugaagca acaguaaggg     1860

gcgcuaaacg uauggcaguu cuaggagaua cggcuuggga uuucggcuca guaggaggag     1920

cacuaaauuc auuaggaaaa ggaauacauc aaauuuuugg agcagcauuc aaaucauuau     1980

uuggaggaau gagcugguuc ucacaaauuu uaauaggcac auugcuaaug uggcuaggau     2040

uaaauaccaa aaaugguuca auauccuuaa uguguuuagc auuaggagga guuuuaauau     2100

uuuuaaguac agcaguauca gcagacguug gauguugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6938
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6938
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaua       60

caucaguugg uauaguagga cuauuguuaa caacagcaau ggcggcagaa guaacaagaa      120

gaggaucagc guacuacaug uaucuagaua gaaacgaugc aggugaagca auaucauucc      180

caacgacccu aggcaugaac aaauguuaua uacaaaucau ggaucuaggu cauaugugug      240

augcaacaau gucuuaugaa ugucccaugu uagaugaagg cguagagccu gaugacguag      300

acuguuggug uaauacaaca aguacauggg uaguuuaugg aacaugucau cauaaaaaag      360

gagaagcaag aagaagcaga cgagcuguga cacuaccuuc acacaguaca agaaaauuac      420

aaacaagauc acaaaccugg uuggaaagca gagaauauac aaaacacuua auacgaguag      480

agaacuggau uuuuagaaau ccagguuucg cauuagcugc agcagcaaua gcauggcuau      540

uagguaguuc aacaucucaa aaaguaauau auuugguaau gauauuacua auagcuccag      600

cuuauaguau cagauguauc ggcguaucaa auagagacuu uguugaaggu augucaggag      660

gaacaugggu cgauauaguu cuugaacaug guggaugugu aacaguaaug gcacaagaca      720

aaccuacagu agauauagaa uuaguaacca caacaguauc aaacauggcu gaaguaagau      780

cuuauuguua ugaagcaagc auaucagaua uggcaagcga cagcagaugc ccaacacaag      840

gagaagcuua ccuagacaaa caaagugaua cucaguacgu auguaaacgg acacuugugg      900

auagagguug gggaaacgga uguggauuau uuggaaaagg caguuuagua acaugcgcaa      960

aauuugccug uucaaaaaaa augaccggaa aaucaauaca gccagaaaau cuagaauacc     1020

guauaauguu aaguguacau gguagucaac auagugggau gaucguaaau gauacaggac     1080

augaaacaga cgaaaacaga gcgaaaguag aaauuacacc uaauucacca agagcagaag     1140

caacauuagg uggauuuggc ucacuuggau uagauugcga accaagaacu ggacuagauu     1200

uuucagauuu auacuauuua acaaugaaua auaaacauug guuaguucac aaagaauggu     1260

uucaugauau accucuaccu uggcacgcgg gagcagacac aggaacaccu cacuggaaua     1320

auaaagaagc uuuaguugaa uuuaaagaug cucacgcaaa acgacaaacu guaguugucu     1380

uagguucuca agaaggagca guacauacug cauuagcagg cgcucuagag gcagagaugg     1440

auggagcaaa agguagauua ucgaguggac auuugaaaug cagauuaaaa auggacaaau     1500

uaagguuaaa aggaguaagu uacucauuau guacagcagc auuuacauuu acaaaaauac     1560

cagcagaaac auuacacgga acuguaaccg uugaaguaca auaugcaggu acagauggac     1620

caugcaaagu accagcacaa auggccguag acaugcagac auugacucca guaggaagau     1680

uaauaacagc aaauccagua auaacugaaa gcacggaaaa uaguaaaaug augcucgaau     1740

uagauccacc auuuggagau aguuacaucg uaaucggagu uggcgaaaaa aaaauuacac     1800

accacuggca uagaucuggu ucaacaauag gaaaagcauu cgaagcaaca guacgcggag     1860

ccaaacgaau ggcaguuuua ggagauacag ccugggauuu uggcagugua ggaggcgcuc     1920

ucaauagucu uggaaaagga auacaucaaa ucuuuggagc agccuuuaag agucuguucg     1980

gaggaaugag uugguucuca caaauacuaa uagguacguu auuaaugugg cuaggauuaa     2040

acacaaagaa ugguucaaua ucuuugaugu gucuagcuuu aggaggcgua cucaucuuuu     2100

ugucaacagc aguaucagcu gauguaggau gcugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6939
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6939
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguaggaau uguaggauua cuacuaacaa cagcuauggc agcagaagua acaagaaggg      120

gcucagcaua uuauauguau uuagauagaa acgaugcugg ggaagcaaua agcuucccua      180

caacauuagg aaugaacaaa uguuacauac aaauuaugga cuuagggcau augugugaug      240

ccacaauguc uuaugaaugc ccaauguuag augaaggagu agagccugac gauguagacu      300

guugguguaa uacaacauca acuugggugg uauauggcac augucaucau aaaaaaggcg      360

aagcacgaag auccaggaga gcuguaacac uaccaucaca uaguacaaga aaauuacaaa      420

caaggucuca aacaugguua gaaucucgcg aauauaccaa acaccuuaua agaguagaaa      480

auuggauauu cagaaaucca ggcuucgcac uagcagcugc agcuauagca ugguuauuag      540

gaucguccac aucacaaaaa gucauauauu uaguaaugau acuauuaauc gcaccagcau      600

auucaauaag guguauagga gugucaaaca gagauuuugu ugaaggaaug uccggaggaa      660

caugggugga cauaguucuu gaacacggag gauguguuac aguuauggcu caagauaaac      720

caaccguuga cauagaauua guaacaacaa cuguaaguaa uauggcagaa guaagaaguu      780

acuguuauga agcaagcaua ucagauaugg caucagacuc aagaugccca acacaaggug      840

aagcauauuu ggauaaacaa ucagauacac aauauguaug uaaaagaaca cuaguagaua      900

gagguugggg gaacggaugu ggauuauuug gaaaaggcag cuuaguaaca ugugcaaaau      960

uugcaugcag caaaaaaaug acaggaaaau cuauacaacc agaaaaucua gaauacagaa     1020

uaauguuguc aguacauggg ucacaacauu caggaaugau aguaaacgau accgggcacg     1080

aaacagacga gaaccgagcg aaaguugaaa uaacaccaaa cagcccaaga gcugaagcaa     1140

cauuaggggg auucggcuca uuaggacuag auugcgagcc aagaacagga uuagacuuuu     1200

cagaucugua uuauuuaaca augaacaaua aacauugguu aguucauaaa gaaugguuuc     1260

acgauauucc auuaccaugg caugcgggag cagauaccgg aacaccacau uggaauaaca     1320

aagaagcuuu aguagaauuc aaagacgcac augcaaaaag acaaacaguu guaguauuag     1380

guagccagga aggggccgua cauacagcac uugcaggagc auuagaggca gaaauggaug     1440

gugcuaaagg cagacuaagu aguggacauc uaaaauguag gcuaaaaaug gauaaauuga     1500

gacucaaagg agucucauau agucuaugua ccgcagcauu cacauuuaca aaaauaccug     1560

cagaaacacu ucauggaaca guaacuguug aaguacaaua cgcagggacu gauggaccau     1620

guaaaguccc agcacaaaug gcaguagaua ugcaaacauu aacaccuguu gguagguuaa     1680

uaacagcaaa cccugugaua acugaaucua cagaaaacag caaaaugaug uuagaguuag     1740

acccaccauu uggagauagu uauauaguca uaggaguagg cgaaaaaaaa auaacucauc     1800

auuggcacag aucaggcagu acgauaggua aagcauuuga ggcaacagua cgcggagcga     1860

aacgaauggc aguacuagga gauacagcau gggauuucgg cucaguugga ggagcauuaa     1920

auucacuugg aaaaggcaua caccaaauau uuggugcagc auucaaaagc cuauuuggag     1980

gaaugucaug guuuagucaa auauuaauag gaacauugcu aauguggcuu ggauuaaaua     2040

caaaaaaugg auccauauca cuaaugugcc uagcauuagg aggaguauua auauuccuau     2100

cuacggcagu gagcgccgau guaggaugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6940
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6940
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gcguaggaau agucggacuu cuauugacaa ccgcuauggc agcagcaguu acaagaagag      120

gcuccgcaua uuacauguac cuagacagaa augaugccgg agaagccaua agcuuuccaa      180

caacacuagg aaugaauaaa uguuacauuc aaaucaugga ucucggacau augugugaug      240

caacaaugag uuaugaaugu ccaauguuag acgaaggagu agaaccugau gaugucgauu      300

gcuggugcaa uacaacauca acaugggucg uauauggcac uugccaccau aaaaagggag      360

aagcuaggcg uucacgaaga gcaguaacac uaccauccca uucaacuaga aaauuacaaa      420

cccgaaguca aacuugguua gaauccagag aauauacaaa acacuugaua agaguagaaa      480

auuggauauu uagaaaucca gguuucgccu uagcagcugc agcaauugca ugguuacuag      540

gaagcucaac aucacaaaaa guuauauacu uaguaaugau acuauuaaua gcuccagcuu      600

acucaauaag auguauagga guuucaaaua gggauuucgu agaagggaug ucaggaggua      660

caugggucga cauaguauua gaacauggag gaugcguaac aguuauggcu caagauaaac      720

caacggucga uaucgaacuu guuacaacua caguaucuaa uauggcagaa guaagaaguu      780

auuguuauga ggcaucuaua agugauaugg cuagugauag uagaugucca acacaaggug      840

aagcauacuu agauaaacaa ucugacacac aauauguuug uaaaagaaca cuaguagacc      900

gaggaugggg caacgggugu ggauuauuug gaaaaggaag uuuaguaaca ugcgcaaaau      960

uugcauguuc aaaaaaaaug acaggaaaau cgauucaacc ugaaaaucua gaauauagaa     1020

ucaugcuuuc aguucacgga ucacagcaua guggaaugau aguaaaugau acaggucacg     1080

aaacggacga aaauagagcc aaaguagaaa uaacuccgaa uucaccaaga gcggaagcca     1140

cucuuggcgg auuuggauca uuaggauuag auugugaacc aaggacagga cuagauuucu     1200

cagaucuaua uuaccuuaca augaauaaca aacauugguu aguacacaaa gaaugguuuc     1260

augauauacc acuaccuugg cacgcaggag cugauacugg uacaccacac uggaacaaca     1320

aagaagcguu aguagaauuu aaagaugcac augcaaaaag acaaaccgua guaguacucg     1380

gaagccaaga aggagcagua cauacagcuc uagcuggagc auuagaagca gaaauggacg     1440

gagcaaaagg aagauuaucu aguggccacc uuaaauguag auuaaaaaug gauaaguuaa     1500

gacucaaagg uguaucauac ucauuaugua cugcugcauu uacauucacg aaaauaccug     1560

cagaaacacu acauggaacu guaacuguug aaguccaaua ugcagguaca gauggaccau     1620

guaaaguacc agcacaaaug gcugucgaua ugcaaacguu aacuccugua ggacgauuaa     1680

uaacagcaaa uccaguuaua acggaaucca cagaaaacuc aaaaaugaug uuagaauuag     1740

auccaccauu uggagauuca uauauaguua ucgguguugg agaaaaaaaa auaacucauc     1800

auuggcauag aagugguuca accauaggaa aagcauuuga ggcaacugua agaggagcaa     1860

aaagaauggc ugugcuggga gauacagcuu gggauuuugg aagugugggc ggagcauuaa     1920

auagcuuagg uaaagguaua caucaaauau uuggagcagc auuuaaauca cuauuuggug     1980

gaaugucaug guuuagucaa auacugauag guacucuuuu gaugugguua ggauuaaaua     2040

caaaaaaugg aagcauaagu uuaauguguu uagcauuagg aggaguauua auauuucuaa     2100

gcacagcagu aucagcugac guaggauguu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6941
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6941
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gagcagauac caguguagga auaguagguc uauuauuaac aacagcuaug gcagcagccg      120

uaaccagaag aggaagugca uauuauaugu accuugacag gaaugaugcg ggugaagcaa      180

uuaguuuucc aacaacacuu gggaugaaua aauguuauau ucaaauuaug gaucuugguc      240

auauguguga ugcaacaaug agcuacgaau guccuaugcu cgaugaagga guagaaccag      300

augauguaga uuguuggugu aauacaacga gcacuugggu aguauaugga accugccauc      360

auaagaaagg ggaagcuaga agaaguagga gagcaguaac acugccauca cauucgacaa      420

gaaaacuaca gacaagaagu caaacauggu uggaaaguag agaauacaca aaacauuuaa      480

uuagaguaga aaacuggaua uuuagaaauc cuggauucgc auuagcagca gccgccauag      540

cuugguuguu aggaaguagc acaagucaaa aagugauaua uuuaguuaug auauuacuaa      600

uagcuccugc auauaguaua agaugcauag gaguaaguaa uagagacuuu guugaaggua      660

uguccggggg aacaugggua gacauaguac uagaacaugg aggauguguu acaguuaugg      720

cacaagauaa accuacagug gauauugaac uuguaacuac aacggucuca aauauggcag      780

aaguacgauc auauuguuac gaagcaagua uaucugauau ggcaucugac ucaagaugcc      840

caacgcaagg agaagcauac cuugauaaac aaagugauac acaauacgua ugcaaaagaa      900

cucuaguaga cagaggaugg ggaaauggcu guggauuauu cggcaaagga ucucuuguua      960

caugcgcaaa auuugccugc ucgaagaaaa ugacaggaaa aaguauucaa ccagaaaauu     1020

uagaauauag aauaauguua agugugcacg guucacaaca uuccgguaug aucguaaacg     1080

auacaggaca ugaaacagau gaaaauagag cuaaaguaga gauuacacca aauaguccac     1140

gagccgaagc aacacucggc ggauuuggca guuuagguuu agauugcgaa ccgagaacag     1200

guuuagauuu uucagauuua uauuauuuaa ccaugaacaa uaaacauugg uuaguccaua     1260

aagaaugguu ucacgacaua ccucuaccau ggcaugcagg agcagauaca gguaccccuc     1320

auuggaauaa uaaagaggcu uuaguagaau uuaaagaugc acacgcaaaa agacaaacag     1380

ugguuguauu agguagccaa gaaggugcag uacauacagc ucuagcagga gcauuagaag     1440

cggaaaugga uggugcaaaa ggaagauuaa guucagggca cuuaaaaugu agauuaaaga     1500

uggacaaguu acgauuaaaa ggaguaucau auagccuaug uacagcggca uuuacauuua     1560

cuaaaauacc agcagaaaca cuacacggaa caguaacagu ugaaguacaa uaugcuggaa     1620

cagauggccc cuguaaagua ccagcacaaa uggcaguaga caugcaaaca cuaaccccag     1680

uaggaagauu aauaacagca aauccgguaa uuacugaauc uacugaaaau agcaaaauga     1740

ugcuagaauu agauccacca uucggcgaca gcuauauagu cauaggggug ggagaaaaga     1800

aaauuacaca ucauuggcac agaucaggaa guacuaucgg uaaagcauuu gaagcaacag     1860

uaagaggagc uaaaagaaug gcaguacuag gggauacagc augggacuuu ggaagugucg     1920

guggagcacu aaauucuuug ggaaaaggga uacaccaaau auuuggugca gcauuuaaaa     1980

guuuauucgg uggaaugucu ugguucaguc agauacuaau uggcacucuu uuaauguggu     2040

uaggucuaaa uacgaaaaau gggucaauau cucuaaugug ucuagcauua ggagguguuu     2100

uaaucuucuu aagcacagca guaucagcag acguaggaug uugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6942
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6942
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gacugucauc aagaaaaaga agaucacaug auguacuuac aguacaauuc uuaauauuag      120

gaauguuacu aaugacagga ggcguaacaa gaagagguag cgccuacuau auguaucuag      180

auagaaauga ugcaggagaa gcaaucaguu ucccuacaac ccuugguaug aauaaaugcu      240

auauacaaau aauggauuug gggcacaugu gugacgcuac aaugaguuac gaauguccaa      300

uguuagauga aggagucgaa ccagaugaug uagauugcug guguaacacc acaucaacau      360

gggugguaua cgggacaugc caucauaaga aaggagaagc aagacguuca agacgagcug      420

uaacauuacc aagucauagc acaagaaaau uacaaacuag aucacaaaca ugguuagaau      480

cccgagagua cacaaaacau cuaauaagag uggaaaauug gauuuuuaga aauccagguu      540

ucgcauuagc ugcagccgcu auagccuggu uauuagguuc aucuacaagu caaaaaguaa      600

uauauuuagu aaugauauua cuaauagcac cagcauauuc uauaagaugu auaggagucu      660

caaauagaga uuucguagaa gguaugagcg gaggaacuug gguagauauu guacuagaac      720

augguggcug uguaacagua auggcucaag auaaaccuac aguagauaua gaauuaguaa      780

caacgacagu aucaaauaug gcagaaguua gaucuuauug uuacgaagca agcaucucag      840

auauggcaag ugacaguaga ugcccaacac aaggggaagc auaucuagac aaacaaucag      900

acacucaaua uguauguaaa agaacuuuag uugaccgagg auggggaaau gguuguggac      960

uauucgguaa aggaucauua guuacuugug cgaaauucgc cugcagcaag aaaaugacag     1020

gaaaaucaau ccaaccagaa aacuuagaau acagaauaau gcuaagugua cauggaagcc     1080

agcacagugg aaugauagua aaugauacag gccaugaaac agaugaaaau agagcaaaag     1140

uagaaauaac accaaauuca ccgagagcug aggcaacacu aggaggauuu gggagcuuag     1200

gauuagauug ugaaccaaga acaggccuag acuuuagcga uuuauauuac uuaacaauga     1260

auaauaaaca cuggcuagua cacaaagaau gguuucauga cauaccuuua ccauggcaug     1320

caggagcaga cacugguaca ccacauugga acaauaaaga agcauuagua gaauuuaaag     1380

acgcccaugc gaaaagacaa acgguaguag uacuaggaag ucaagaaggg gcaguacaua     1440

cagcuuuagc uggugcauua gaagcugaaa uggauggagc aaaagguaga cuauccucag     1500

gucacuuaaa auguagacua aaaauggaca aauuaagguu gaaaggagua ucauacaguu     1560

uauguacagc agcauuuaca uuuacuaaaa uaccugcaga gacacugcau ggaaccguua     1620

caguggaagu acaauacgca ggaacagaug gaccauguaa aguaccagca caaauggcgg     1680

uagauaugca aacacuaacc ccgguaggua gauuaauaac agcaaacccu gugauaacag     1740

aaagcacuga aaacucaaag augauguuag aauuagaccc accauucggu gauaguuaua     1800

uaguaauagg agucggagaa aagaaaauaa cacaucacug gcauagaagu gguucaacaa     1860

uagguaaagc guucgaagca acaguucgag gagcaaaaag aauggccgua cuaggagaua     1920

cugcauggga cuuuggauca guaggaggag cuuuaaacuc ucuagguaaa gguauucauc     1980

aaauauucgg ggcugcuuuu aagagcuuau uuggcggaau gucuugguuu ucacaaauau     2040

uaauaggaac acuauuaaug uggcuaggac uaaauacgaa aaauggaagc auaagucuga     2100

uguguuuagc acugggagga guacucaucu uccuaagcac agcaguuuca gccgauguag     2160

gauguugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6943
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6943
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gacuaucauc aagaaaaaga agaucacaug acguccuuac aguacaauuu uuaauauuag      120

gaauguuauu aaugaccgga gcaguaacua gacgaggauc agcauauuac auguaccuag      180

accgaaauga cgcaggagaa gcuauaucau uuccuacaac ccuaggaaug aacaaauguu      240

acauacaaau aauggauuua ggacauaugu gugaugccac aaugucauau gaauguccaa      300

ugcucgauga aggaguagaa ccggaugacg ucgauuguug gugcaacaca acuucgacuu      360

ggguaguaua cgguacuugu caucauaaga aaggagaagc gagacguuca aggagagcag      420

ugacacuacc gucucacuca acaagaaaau uacaaacaag aucacaaacg ugguuagaaa      480

gcagagaaua uacaaaacac uuaaucagag uggaaaauug gauauuuagg aacccaggcu      540

ucgcuuuagc agcggcagca auagcauggu uacuaggauc cagcacauca caaaaaguaa      600

uauacuuggu aaugauauua cuaauugcuc cagcauacuc aauuagaugu auagguguaa      660

guaacagaga uuucguugaa ggaaugagug gaggaacaug ggucgauaua guacuugagc      720

auggcggaug cguaaccgua auggcacaag auaagccaac uguagauaua gaacugguua      780

caacaacagu cucuaauaug gcagaaguaa gaagcuauug uuaugaagcg ucaauaucag      840

auauggcguc agacucaaga ugccccaccc aaggagaagc auaucuagau aaacaaagug      900

acacacaaua uguaugcaaa aggacucuag uagauagagg auggggaaau ggguguggau      960

uauucggaaa agggucccua guaacaugcg caaaauuugc cugcucaaag aaaaugacag     1020

ggaaaucaau ucaaccagaa aaccuagaau acagaauaau guuaagcguu cauggaaguc     1080

aacauucggg uaugauugua aacgauacag gacaugaaac ugacgaaaau agagcgaaag     1140

uugaaauaac accuaauuca ccaagagcag aagcaacucu cggaggauuc ggaagucuug     1200

gacuggauug cgaaccacga acaggguuag auuuuagcga ccuauauuau cuaacgauga     1260

auaacaaaca uugguuagua cacaaagaau gguuucauga caucccacua ccauggcaug     1320

caggagcaga cacagguacg ccacauugga auaacaaaga agccuuagua gaauuuaagg     1380

augcacaugc aaaaaggcaa acuguaguug ucuuaggaag ccaagaaggc gcaguacaua     1440

cagcauuagc uggagcuuua gaggcugaaa uggauggagc aaaagguagg uuaucaagug     1500

gacauuuaaa augucgauua aaaauggaua aauuaagguu aaaaggagua ucuuauucau     1560

uaugcacagc agcauuuaca uuuacaaaaa uaccagcaga aacccuacau gguacaguaa     1620

caguagaagu ccaauaugca ggcacagaug gaccaugcaa aguuccagcu caaauggccg     1680

uagauaugca aacuuuaaca cccguuggaa gacuaauaac agcaaaucca guaauaacag     1740

aaucaacaga aaauaguaaa augaugcuag aacuagaucc cccauuuggc gauagcuaua     1800

uagucauagg ggucggagaa aagaaaauua cacaucacug gcaucgauca ggcaguacaa     1860

uagguaaagc auuugaagca acaguacgag gagcuaaaag aauggcaguc cuaggagaua     1920

cagcuuggga cuuugguuca guaggaggag cauuaaacuc auuaggaaaa gguauacauc     1980

aaauauuugg agcagcuuuc aaaucauuau uuggaggcau gucuugguuu agucagauac     2040

uaauaggaac ucuauuaaug ugguuaggcu uaaauacuaa aaauggaagc auaucuuuaa     2100

ugugucuagc uuuaggugga guauuaauau uuuuaagcac agcuguauca gcugauguag     2160

gcuguugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6944
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6944
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uaauaaaugc ccgcaaagaa aagaaacgga gaggagccga uacaucugua gguauuguag      120

gauuauuauu aacaacagcg auggcagcag aaguaacaag aagaggauca gcuuauuaua      180

uguauuuaga cagaaacgau gcaggagaag caauuagcuu uccaacuaca uuagguauga      240

auaaauguua uauacaaaua auggauuuag gacauaugug ugaugcgaca auguccuaug      300

aauguccaau gcuagaugaa gguguagagc cagaugaugu agauugcugg uguaauacaa      360

caucaacaug ggucguauau ggaacaugcc aucauaagaa aggugaagca aggcguagua      420

ggagagcagu uacauuacca ucucauucua cuagaaaacu acaaacaaga ucacaaacau      480

gguuagaaag ucgagaauau acuaaacauu uaauaagagu agaaaacugg auuuuuagaa      540

acccaggauu ugcacuagcu gcagcugcaa uagcaugguu guugggaagc aguacaaguc      600

agaaaguaau uuauuuagua augauauuau uaauugcacc agcauauuca auuagaugua      660

uagguguauc uaacagagac uuuguagagg gcaugucagg aggaacaugg guugauauug      720

uuuuagaaca ugguggaugu guaacuguaa uggcucaaga uaaaccaaca guagacaucg      780

aauuagugac uacaacagua ucaaacaugg cugagguuag auccuauugu uaugaagccu      840

caaucagcga uauggccagu gauaguagau guccaacaca aggugaagca uauuuagaua      900

aacaaaguga uacgcaauau guuuguaaaa ggacguuagu agaucggggc uggggaaacg      960

gaugcggcuu auuuggaaaa ggaaguuuag uaacaugugc aaaauuugca ugcucaaaga     1020

aaaugacagg uaaaagcauu cagccugaaa auuuagaaua uagaauaaug cuaaguguac     1080

auggcaguca acauucagga augauuguaa augacacggg acacgaaaca gacgaaaaca     1140

gagcaaaagu cgaaauaacg ccuaauaguc caagagcaga agcaacuuua gguggauuug     1200

gaagccuugg acuagauugu gaaccacgaa cugguuugga uuucagugau uuauacuauu     1260

uaacuaugaa uaacaaacac ugguuaguac auaaagaaug guuucaugac aucccauuac     1320

cauggcaugc aggagcggau acugggacac cacauuggaa caauaaagag gcguuagucg     1380

aguucaaaga ugcacaugcg aaacgacaga caguaguagu auugggaagu caagaaggag     1440

ccguacacac agcauuagcu ggagcauuag aagcugaaau ggacggagca aaaggaagau     1500

uaucaucugg acacuugaaa uguagauuaa aaauggauaa acucagacug aaaggaguaa     1560

gcuacagucu auguacagca gcauucacau uuacaaaaau uccugcagaa acacuacaug     1620

gaacaguuac aguagaagua caauaugcag gaacagaugg accauguaaa guaccggcac     1680

aaauggcugu agacaugcaa acacuuacgc cuguaggaag guuaauuaca gcaaacccug     1740

ugauaacaga aaguacggaa aauucaaaaa ugauguuaga acuagacccu ccauucggug     1800

auaguuauau agucauagga guaggugaaa agaaaauuac acaccauugg caucgcucag     1860

gcucaacaau aggaaaagcg uuugaagcga caguaagggg agcaaaaaga auggcagugu     1920

uaggggauac agcaugggau uucggaucgg uaggaggugc auugaauagu cuaggaaaag     1980

guauucacca aauauuugga gcagcguuca aaucauuauu uggggguaug aguugguucu     2040

cacaaauauu aauagggacc uuauuaaugu ggcuaggacu aaauacgaaa aaugguucaa     2100

uuagucucau guguuuagca uuagguggag uauugauauu ccuaaguaca gcaguauccg     2160

cagauugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  6945
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6945
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

uuaacgcaag aaaagaaaaa aagagaaggg gggcagacac aucuguagga aucguagggu      120

uauuauuaac aacagcuaug gcagccgaag uaacgcgaag aggaucagcu uauuauaugu      180

auuuagacag aaacgaugca ggagaagcaa uaaguuuucc aacuacucua ggaaugaaua      240

aauguuacau acaaauaaug gauuuaggac acauguguga cgcaacaaug agcuaugaau      300

gcccaauguu ggaugaaggc guagagcccg augaugugga uuguuggugu aauacaacaa      360

gcacuugggu gguauauggc acuugccauc acaaaaaagg cgaagcaaga aggucuagaa      420

gagcaguaac auuaccaucc cacucaacaa gaaaauuaca aacaagaucc caaacauggc      480

uagaaucuag agaauauaca aaacauuuaa uucgcguaga aaauuggaua uucagaaauc      540

cgggguuugc acuagcagca gcugcaauag cauggcuauu aggaucaucg acaagccaaa      600

aaguaauaua uuuaguaaug auacuacuca uagcaccugc auacucaaua agauguaucg      660

guguaucaaa uagagauuuc guggaaggca ugaguggugg aacauggguu gacauaguuu      720

uagaacaugg agguuguguu acuguaaugg cucaagauaa accaacagua gauauagaac      780

ucguaacaac aacaguuuca aauauggcgg aaguaagaag uuauuguuau gaagcuucua      840

uuucagauau ggccagcgac agucguugcc caacacaagg ugaagcauau cuagacaagc      900

aaagugauac ucaauaugua ugcaaaagaa cacuaguaga cagagguugg gguaauggau      960

gugguuuauu uggaaaagga agccuaguaa caugcgcaaa auuugcuugc aguaaaaaaa     1020

ugacagggaa aaguauacag cccgaaaacc ucgaguauag aauaauguua ucuguacaug     1080

gaucacaaca cagcggaaug auaguaaaug auacaggaca cgaaacagau gaaaacagag     1140

caaaagucga aauaacacca aauaguccuc gagcugaagc uacauuagga ggauucggau     1200

cgcuaggguu agauugcgaa ccuagaacug gauuagacuu uucugauuua uauuaccuga     1260

caaugaacaa caaacacugg cuuguacaua aagaaugguu ccaugauauu ccauuaccau     1320

ggcaugccgg agcagauaca ggaacccccc auuggaauaa uaaagaagca uuaguagaau     1380

ucaaagacgc gcacgcuaaa agacaaacag uaguaguacu aggaucacaa gaaggagcug     1440

uacauacagc auuggcagga gccuuagaag ccgaaaugga cggagcaaaa ggucgucuuu     1500

caagcggaca uuugaaaugu agacuaaaaa uggauaaacu cagacuaaaa gggguaucau     1560

auucacuaug uacagcagca uuuacauuua caaaaauacc agcagaaaca uuacauggua     1620

cagucacagu agaaguucaa uaugcaggaa cagauggucc guguaaagua ccugcacaaa     1680

uggcaguaga uaugcagaca uuaacaccgg uugguagguu aauuaccgca aauccaguaa     1740

uaacagaaag uacagaaaau ucaaaaauga uguuagaauu agauccacca uuuggagaca     1800

gcuauauagu aauaggagua ggugaaaaaa aaauaacaca ucauuggcau agaaguggau     1860

caacaauagg aaaagcauuu gaagcaacgg ucagaggagc aaaaagaaug gccguuuuag     1920

gggauacagc augggauuuu ggaaguguag guggagcauu aaacucuuua gguaaaggca     1980

uacaucaaau auuuggagcg gcuuuuaaaa guuuauucgg ugguauguca ugguuuaguc     2040

aaauacuaau aggaacuuua uuaauguggu ugggauuaaa uacaaaaaac gguucgauua     2100

guuuaaugug cuuagcauua ggcggaguau uaauauucuu aaguacggcg guaagugcug     2160

auugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6946
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6946
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

augcaaggaa agaaaaaaaa agaagaggag cagauacauc cguuggaaua guuggacugc      120

uauuaacaac agcuauggca gcagagguua cacgaagagg aagcgcauau uauauguauc      180

uagauagaaa ugaugcagga gaagcuauaa guuuuccgac aacauuagga augaauaaau      240

guuauauaca aauaauggac cuaggacaua ugugugaugc aacaaugagu uaugaauguc      300

caauguugga cgaaggagua gaacccgacg acguagauug uugguguaau acaacaagca      360

ccuggguagu auauggcaca ugucaccaua agaaaggaga agcaagaaga ucacgaagag      420

cgguaacauu accaucacau ucuacacgaa aauuacaaac aaggucacaa acaugguuag      480

aaagcagaga auauacaaaa cauuuaauuc gaguggaaaa uuggauauuu agaaauccag      540

gauuugcacu agccgcagca gcaauagcau gguuacuagg aucaucaaca ucacaaaaag      600

uaauauauuu aguaaugaua cuacuuauag caccagccua cucaauuaga ugcauuggag      660

uaucuaauag agauuucgua gaaggaauga gcggaggaac uuggguggau auuguauuag      720

aacacggagg auguguaaca guaauggcuc aagauaagcc aacaguagac auagaauuag      780

uaacaacuac aguaaguaau auggccgagg ucagaagcua cugcuaugaa gcaucaauuu      840

cagauauggc aagcgauuca agauguccaa cacaagguga agcuuauuug gacaaacaaa      900

gugacacaca auauguaugc aaacgaacac ugguagaucg cggaugggga aauggaugug      960

gauuauuugg aaaaggguca uuaguaacau gugcaaaauu cgccuguuca aaaaaaauga     1020

cuggaaaauc cauucaaccc gaaaaucuag aauauagaau aauguuaagu guacauggau     1080

cacaacauuc ugguaugaua guaaaugaua caggucauga aacagaugaa aaccgcgcaa     1140

aaguagaaau aacaccuaac ucaccuagag cagaagcaac acuaggagga uuuggaagcu     1200

uaggguugga cugugaaccu agaacaggac uggacuuuuc ugacuuauau uaucuaacaa     1260

ugaacaauaa acauugguua guacauaaag aaugguucca ugauauacca uuaccauggc     1320

augcaggagc ugauacagga acaccucacu ggaacaauaa agaagcacuc guagaauuua     1380

aagaugccca ugcgaaaaga caaacuguag uaguuuuagg aucccaagaa ggcgcuguac     1440

acacugcacu agcuggggca uuagaagccg aaauggacgg ugcaaaggga cgauuaucau     1500

ccggacauuu gaaaugucgu uuaaaaaugg acaaauuaag acuaaaagga guaucguauu     1560

cacuauguac agcagccuuu acuuuuacaa aaauaccagc agaaacauua cauggaacag     1620

uuacaguuga aguacaauau gccggaacag acggaccaug caaaguucca gcacaaaugg     1680

cuguagauau gcaaacauua acuccaguag gcagauugau aacagcaaac ccugucauaa     1740

cagaaucaac ugaaaacagu aaaaugaugc uugaacuaga ccccccauuu ggagacucau     1800

acauaguaau agggguaggu gaaaaaaaaa uaacacacca uuggcauaga aguggaucca     1860

caauagguaa agccuuugaa gcaacuguac guggagcaaa aagaauggca gucuuagggg     1920

auacagcaug ggauuuugga ucaguaggag gagcuuuaaa cucuuuagga aaaggaauac     1980

aucaaauauu uggagcagca uucaaaucac uuuuuggagg aaugucaugg uuuucucaaa     2040

uucuaaucgg gacacuacuu auguggcuag gauuaaauac caaaaacggc aguauaucac     2100

ugauguguuu ggcauuaggu ggaguuuuaa uauuuuugag cacagcggug ucagcagauu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6947
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6947
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaug       60

cgaggaaaga aaagaaaaga agaggugcag auacaucagu cggaauagua ggacuguuau      120

ugacaacagc gauggcagca gaaguaacaa gaagaggaag cgcauauuau auguacuuag      180

auagaaacga ugcuggugaa gcaauaucau uuccuacaac auuaggaaug aacaaauguu      240

auauacaaau aauggauuug ggacauaugu gugaugcuac aaugaguuau gaauguccaa      300

uguuggauga aggaguagag ccagacgaug uagauuguug guguaacaca acaucaacau      360

ggguaguaua ugggacuugu caccauaaga aaggggaagc ucgacgaagc cguagagcgg      420

uaacgcuacc aagccauagc acaagaaaau uacaaacgag aagucaaacu ugguuagaaa      480

gcagagaaua cacaaaacau uuaauaagag uagaaaauug gauauucaga aacccagggu      540

uugcacuagc ggcggcugca auagcauggc uccuaggauc aucaacauca cagaaaguaa      600

uauaccuagu aaugauacua cuaauagcuc cugcauauag uaucagaugu auaggaguaa      660

guaaucgaga uuuuguagaa gguauguccg guggaacgug gguagacaua guacuagaac      720

auggaggaug uguaacaguc auggcacaag acaaaccgac aguagacaua gaauuaguua      780

cgacaacagu aucaaauaug gcagaaguga gaucuuauug uuacgaagca agcauaagcg      840

acauggcaag ugacagccgu uguccaacac aaggagaagc auacuuagau aaacaaagug      900

auacacaaua uguauguaaa agaacgcuag uugacagggg cuggggaaac ggaugcggcu      960

uauucggaaa aggaucucua guuacaugug caaaauuugc auguucaaag aaaaugacag     1020

gcaaaucaau acaaccggaa aauuuagaau auaggauaau guuaucagug caugguucac     1080

aacauucagg aaugauaguu aacgauaccg gucaugagac agacgaaaau agagcaaaag     1140

uagaaauaac acccaacuca ccaagagcag aagcaacacu agguggauuc gguagcuuag     1200

gauuagacug ugaaccaagg acaggauugg auuuuaguga ccuauauuac cuaacaauga     1260

auaauaaaca cuggcuagua cauaaagaau gguuucauga uauaccguua ccuuggcaug     1320

cuggugcuga uacagggacu ccacauugga auaauaaaga agcauuagua gaauuuaaag     1380

augcgcacgc gaaaagacaa acgguaguag uauuaggguc acaggaagga gcaguacaua     1440

cugcacuagc aggagcauua gaagcagaaa uggauggagc uaaaggaaga cucagcucag     1500

ggcacuuaaa augcagauug aaaauggaua aauuaagacu uaaagguguu aguuauucuc     1560

ucuguacugc agcauuuacu uucacuaaaa uaccggcaga aacacuucau ggcacaguaa     1620

cagucgaagu acaguacgca ggaacagaug ggccauguaa aguaccagca caaauggcug     1680

uagauaugca aacguuaaca ccuguaggac gauuaauaac cgcaaacccc guaauuacag     1740

aaucaacaga aaacucaaaa augaugcuug aauuagaccc accauucgga gauagcuaua     1800

uaguaauagg agugggagaa aagaaaauaa cacaucauug gcauagaagc gguucuacaa     1860

uagguaaagc auucgaggca acgguuagag gggcaaaaag aauggcggua uuaggagaca     1920

cagcuuggga uuucggaagc guagguggag cauuaaauuc acuuggaaaa gguauacauc     1980

aaauauuugg agcagcauuc aaaaguuuau ucggagguau gucaugguuu agucaaauuu     2040

ugauaggaac auuacugaug ugguuagggu uaaauacuaa aaacggaagc auuagcuuaa     2100

ugugucuugc ucuaggaggc guauuaauau ucuuaagcac agcaguuagu gcagacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6948
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6948
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcua       60

gaaaagaaaa gaaacgaaga ggcgcagaua caucaguagg aauaguuggu cuauuacuaa      120

caacugcaau ggcagcagaa guaaccagaa ggggaagugc cuauuauaug uaucuugaua      180

ggaaugaugc uggugaggca auaucauuuc cuacaacucu uggaaugaau aaaugcuaca      240

uacaaauaau ggauuuaggu cauaugugcg augccacaau gucauacgaa ugcccaaugc      300

uagaugaagg aguagaaccu gaugauguag acuguuggug caacacaacc aguacauggg      360

uaguauaugg aacuugucau cacaagaaag gagaagcaag aagaaguaga cgagcaguaa      420

cgcuaccaag ccacucaaca cggaaauuac aaacaagaag ccaaacaugg uuagaaagca      480

gagaguauac gaaacaccuc aucagaguag aaaacuggau auuuagaaac ccgggauuug      540

cauuagcagc agcggcaaua gcauggcuau uaggaaguuc cacaagucaa aaaguuaucu      600

acuuaguaau gauacuuuug auagcaccag cauacucaau uagauguauc gggguuucua      660

auagagauuu uguagaagga augaguggag guacaugggu agauauagua uuagaacacg      720

gaggaugugu cacaguaaug gcacaagaua aaccaacagu ugacauagaa uuagugacaa      780

caacaguaag caauauggca gaaguacgau ccuacuguua cgaagcaagu auaagcgaua      840

uggcuaguga cucacgaugu ccuacucaag gugaggcuua ccuagauaaa caaagcgaua      900

cgcaguaugu augcaaaaga acauuaguag acaggggaug ggguaacgga ugcgggcuau      960

uuggaaaagg cucucuugua acaugugcaa aauucgcuug uaguaagaaa augacgggua     1020

aaucaauaca accagaaaau cuagaauaua gaauaauguu aaguguacau ggaucucaac     1080

auagugggau gaucguaaau gauacagguc acgaaacaga cgaaaacaga gcuaaaguag     1140

aaauaacacc aaauagcccg agagcagaag cuaccuuggg aggauuugga agccuagguc     1200

uugauuguga acccagaaca ggauuagauu ucucagauuu auauuaccua accaugaaua     1260

auaaacauug gcugguacau aaagaauggu uucaugauau accuuuacca uggcacgcgg     1320

gggcagacac cgguacucca cacuggaaua auaaagaggc auuaguagaa uuuaaagacg     1380

cacaugcaaa aaggcaaaca guagugguac uagguagcca agaaggagca guacauaccg     1440

cauuagcagg agcacuagag gcugaaaugg auggagcaaa aggaagacua ucaucaggac     1500

aucuuaaaug ucgauuaaaa auggacaaau uaagauuaaa agggguaagc uauaguuuau     1560

gcacggcagc uuuuacauuu accaaaauac cagcagaaac cuuacauggu accguaaccg     1620

uugaaguuca auaugcugga acagacggac cauguaaagu acccgcacaa auggcaguag     1680

auaugcaaac acuaacacca guugggcgac ugauaacugc aaauccagua auaacagagu     1740

caacagaaaa cucaaagaug auguuagaau uagauccacc auuuggugau ucauauauag     1800

uaauaggagu uggagaaaag aaaaucacac aucauuggca uagaagcgga aguacuauag     1860

gaaaggcuuu ugaagcaaca guaagaggag caaaacgaau ggcagucuua ggagauacug     1920

cuugggauuu cggaagugua ggaggagcuu uaaacagccu cgguaaaggg auacaucaaa     1980

uauuuggagc agcauuuaaa aguuuauuug gaggaaugag uugguuuuca caaauauuaa     2040

uuggcacauu acuuaugugg cuagguuuaa auacgaaaaa cggaagcaua uccuugaugu     2100

gcuuagcacu uggaggggua cuaauauucu ugaguacagc aguaagugca gauugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6949
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6949
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aagagaaaaa acgaagaggc gcagauacua guguaggcau cguaggacua uuauuaacaa      120

cugcuauggc agcagaaguu acaagaagag gcagcgcaua uuacauguau uuagaccgaa      180

augacgcagg agaagcuauu ucauucccaa caacauuagg aaugaacaaa uguuauauac      240

aaauuaugga cuuaggccac augugugaug caacaauguc auaugaaugu ccaauguuag      300

augaaggagu agaaccagau gauguagacu guuggugcaa uacaacaagu acauggguag      360

uauacgguac augucaucau aaaaaaggug aagcaagaag aucuaggaga gcaguaaccu      420

uaccaucaca uucaacaaga aaauuacaga caagaaguca aacgugguua gaaaguagag      480

aauauacaaa acaucuaauu aggguagaaa auuggauauu cagaaauccg ggauuugcau      540

uagcagccgc agcaauagca uggcuauuag ggucuucaac aagucagaaa guaauuuacc      600

uaguaaugau auuacuaaua gcaccggccu acaguauacg guguauagga guaaguaaca      660

gggauuucgu ugaaggaaug aguggaggua cauggguaga cauaguauua gaacauggug      720

gauguguaac uguuauggca caagauaaac cuacaguaga cauugaacua guuacaacaa      780

caguuaguaa cauggcagaa guaaggaguu auuguuauga agcuucaaua agugauaugg      840

caucagauuc acguuguccu acacaaggag aagcuuauuu agauaaacaa agugauacac      900

aauacguaug uaaaagaaca uuaguagauc gaggaugggg aaaugguugu ggucuauucg      960

gcaaagguuc uuuaguaacc ugugcaaaau uugcuugcag caaaaaaaug acuggaaaaa     1020

guauccaacc agagaacuua gaauauagaa uaauguuaag uguacaugga agccaacacu     1080

caggaaugau aguaaaugau acaggacaug aaaccgauga gaauagagcu aaaguagaaa     1140

uaacaccaaa cucaccucga gcugaagcaa cauuaggagg auucggaucg uugggauuag     1200

acugugaacc gagaacugga cuagauuuuu cugauuuaua uuauuuaaca augaauaaua     1260

aacacugguu aguucauaaa gaaugguuuc augauauacc auuaccuugg caugcaggug     1320

cugauacugg aacaccacac uggaauaaua aagaagcauu aguagaauuu aaggaugcac     1380

acgcgaaaag acaaacagua guaguuuuag gaagccaaga gggagcagua cacacagcuc     1440

uagcaggagc guuagaagca gaaauggaug gugcaaaagg acgauuauca ucaggacauc     1500

uuaaaugucg acuaaaaaug gacaaacuga gacuaaaagg aguaucauau aguuuaugua     1560

cagcagcguu uacauuuacu aaaauaccug cggaaacacu ucauggaacu guuacgguag     1620

aaguacaaua ugcaggaacu gauggaccau gcaaaguacc ggcccaaaug gcaguagaca     1680

ugcaaacuuu aacaccaguc ggaagauuaa uaacugcaaa ccccguaaua acagaaagca     1740

cugaaaacag uaaaaugaug uuagaacuug aucccccauu cggagauagc uauauaguua     1800

uaggcguagg agaaaagaaa auaacacauc auuggcacag aucaggauca acaauaggaa     1860

aagcuuuuga agcuacagug agaggagcaa aaagaauggc aguauuggga gauacagcuu     1920

gggauuucgg uucuguuggc ggagcgcuaa auagucuagg aaaaggaaua caucaaauau     1980

ucggugcugc auuuaaauca uuguucggag gcaugucaug guuuagccaa auuuuaauag     2040

gaacacuauu aaugugguua ggauuaaaca ccaaaaaugg cucuauuuca cuaaugugcu     2100

uagcauuagg agguguauua auauucuuaa guacagcggu uagugcggac ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6950
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6950
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaag       60

agaaaaaaag aagaggagca gauacgucag uaggaauugu aggauuauua uugacaacag      120

caauggcagc ugaagugacu cguagaggau cagcauacua uauguauuug gaucguaaug      180

acgcagguga agcaauuucu uuuccuacaa cauuagguau gaauaagugu uauauacaaa      240

uaauggacuu aggacacaug ugugaugcaa caaugucaua ugaaugcccu auguuagacg      300

aagggguaga accagacgau guugauuguu gguguaauac uacaucaaca uggguaguau      360

acggaacaug ucaccauaaa aaaggagaag cacgcagaag uagacgagca guaacacuac      420

cuucacauuc aacaagaaaa cuacagacga gaucacaaac uuggcuagaa aguagagaau      480

acacaaaaca ucuaauaaga guagaaaauu ggaucuuuag aaauccaggu uuugcguuag      540

cagcagcagc aauagcuugg uuguuagggu cgucaacuag ccaaaaagua auauaucuag      600

uaaugauauu auuaaucgca ccggcuuaua guauaagaug cauaggaguu aguaaucgag      660

auuuuguaga gggaauguca ggaggaacuu ggguagauau cguucuagaa cacggaggau      720

guguaacagu aauggcacag gauaaaccaa caguugauau ugaauuagua acaacaacag      780

uaagcaacau ggcagaagua aggaguuauu gcuacgaagc uaguauauca gauauggcaa      840

gugauucaag auguccuaca caaggugaag cauauuuaga uaaacaaagc gacacacaau      900

augucuguaa aagaacauug guugauagag guuggggaaa ugguugcgga uuauucggca      960

aagguucucu aguaacuugc gcaaaauuug cauguucaaa aaaaaugaca gguaaaagca     1020

uacaaccaga aaacuuagaa uauaggauua uguuaucggu ucauggcucg caacauagcg     1080

guaugauugu aaaugauaca ggacacgaaa cagacgaaaa cagagcaaaa guagaaauaa     1140

cuccaaauag cccaagagca gaagccacac uagguggauu ugguaguuua ggacuagauu     1200

gugaaccaag aacaggauua gauuuuagug accuauauua uuuaacaaug aauaauaaac     1260

acugguuagu ucauaaagaa ugguuucacg auauuccauu accuuggcau gcaggugcgg     1320

acacaggaac gccacauugg aacaauaaag aagcuuuagu agaauucaaa gaugcucaug     1380

caaaaagaca aacaguagua guacuaggaa gucaagaagg agcgguacau acagcacuag     1440

caggugcauu agaagcagaa auggauggag cgaaaggcag acuaucaucu ggacaucuaa     1500

aauguagauu aaaaauggac aaacuaaggc uaaaaggagu aaguuacuca cuauguacag     1560

cagcauucac auuuacaaaa auuccagccg aaacguuaca cggaacagua acggucgaag     1620

ugcaauaugc aggaacugau ggacccugua aaguaccugc ucaaauggca guagauaugc     1680

aaacacuuac accaguagga agauuaauaa cagcuaaucc aguaauaaca gaaucuacag     1740

agaacucaaa aaugaugcuc gaauuagacc caccauucgg ugauaguuau auaguaauag     1800

gaguaggaga aaagaaaaua acacaucacu ggcacagauc agguucaaca auuggaaagg     1860

cauuugaagc uacuguuaga ggagcaaaac gaauggcagu auugggugac acggcauggg     1920

auuuuggauc aguaggagga gcuuugaauu cucuaggcaa aggaauacac caaauauucg     1980

gagcagccuu caaaucucua uuuggaggua ugagcugguu cucacaaaua uuaauaggua     2040

cuuuauuaau gugguuagga uuaaacacua aaaauggauc aauaucccua auguguuuag     2100

cguuaggagg uguauuaaua uuccuaagua cagcugucag cgccgauuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6951
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6951
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaaa       60

aaaaaagacg aggggcggau acaucaguag gaauaguugg uuuauuauua acaaccgcaa      120

uggcagcaga aguaacuaga agaggaucag cauauuauau guauuuagau agaaaugaug      180

caggagaggc aauaucauuu ccuacaacac uuggaaugaa uaaauguuac auacagauua      240

uggauuuggg acacaugugu gacgcaacaa ugaguuacga augcccaaug cuagaugaag      300

ggguagaacc agacgacgug gauuguuggu guaacacaac cucaacgugg gucguauacg      360

gaacaugcca ccauaaaaaa ggagaagccc gacggagucg aagagcagug acauuaccaa      420

gucauucaac gagaaaauua caaacuagaa gccaaacaug guuagaaagc cgagaauaca      480

caaaacaucu uauucgagua gaaaauugga uauuuagaaa ucccggguuu gcacuagcag      540

cagcagcaau agcaugguua cuaggaucau caacauccca aaaagugauc uacuuaguaa      600

ugauauugcu uauagcgcca gcauauagua uaagguguau cggaguaagu aauagagauu      660

ucguagaagg uaugagugga ggaacauggg uugacauagu uuuggagcau ggcgggugcg      720

uaacaguaau ggcacaagac aaacccacgg uagacauaga auuagugacu acuacuguau      780

caaauauggc agaaguuaga agcuacuguu acgaagcauc aauaucugac auggcaagug      840

auaguagaug uccaacccaa ggagaagcau aucuagauaa acaaucagau acacaauacg      900

uauguaaaag aacucuugua gaucgagguu ggggaaaugg uugcggcuua uuuggaaaag      960

gaaguuuagu aacaugugca aaauuugcau guucuaaaaa aaugaccggu aaaucgauac     1020

agccggaaaa ccuagaauac cgaauuaugu uaaguguaca ugguucgcag cauucaggaa     1080

ugauagugaa cgacacaggu caugaaacag augaaaaucg ugcuaaggua gaaauuacac     1140

caaacucgcc aagagcagaa gcaacacuag gaggauuugg uucacuggga uuggauugug     1200

aaccuagaac aggccuugau uucucagauu uauauuauuu aacaaugaau aauaaacauu     1260

ggcuuguaca uaaagaaugg uuucaugaua uaccauuacc auggcacgcu ggagcagaca     1320

cggguacacc acauuggaac aacaaagaag cacuaguaga auuuaaggac gcccaugcaa     1380

aaagacaaac ugugguagua cuagguaguc aagaaggagc uguacauacu gcucuggcag     1440

gggcauuaga agcugaaaug gauggugcaa aaggaagauu aucaagugga cacuuaaaau     1500

gcagacuaaa aauggacaaa uuaagauuaa aaggaguuuc auauaguuua uguacagcag     1560

cauuuacuuu uacaaaaauu ccagcagaaa cauuacaugg cacaguaacu guagaagucc     1620

aauaugcagg aacagacgga ccuuguaaag uuccagcaca aauggcagua gacaugcaaa     1680

cguuaacacc aguuggaaga cuuauaacug caaauccagu aauaacagaa aguacagaga     1740

auaguaaaau gaugcuagaa uuagauccac cuuucggaga uucauauauu guaauaggug     1800

uaggagaaaa aaaaauaaca caccacuggc aucgaagugg uaguacuaua ggaaaagcau     1860

uugaagcuac agucagggga gcaaagagaa uggccguacu aggagauaca gcaugggacu     1920

ucggaagcgu uggaggugcc cuaaauucau uagggaaagg uauacaccaa auauucggcg     1980

ccgcauuuaa aagccuauuu ggaggaauga guugguuuuc acaaauacua auaggcacau     2040

uauuaaugug guuaggcuua aacacaaaaa auggaucaau aagucuaaug uguuuagcau     2100

uaggaggagu auuaauuuuu cuaucaacag cuguaucugc ugacugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6952
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6952
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

aaagaagagg agcagacaca aguguaggaa uaguaggacu auuauuaaca acugcaaugg      120

cagcagaagu aacaagaaga ggaucagcgu auuauaugua ucucgauaga aaugaugcag      180

gagaggcaau auccuuccca acaacucuug gaaugaacaa augcuauaua caaauuaugg      240

auuuaggaca uaugugcgau gcaaccaugu ccuacgaaug uccaauguua gaugaaggug      300

uagaaccuga ugauguagau uguuggugua auacaacaag uacuugggug guauauggaa      360

caugccauca uaaaaaagga gaggcaagaa gaagcaggag agcgguaaca cuaccauccc      420

auagcacacg aaaacuacaa acuagaaguc aaacauggcu agaaagcaga gaauacacua      480

aacaucuuau aagaguagaa aauuggauau uuagaaaccc aggguuugcg cuagcagccg      540

cagccauugc uugguuauua ggcucaucaa caagccaaaa aguaauauac cuaguaauga      600

uacuacuaau agcgccugca uauucaauua gaugcauugg agucuccaau agagauuuug      660

uagaaggaau gaguggaggg acaugggucg acauaguacu agaacacgga gguuguguua      720

cagugauggc gcaagauaaa ccaaccgucg auauagaauu aguaacuaca acaguaucaa      780

auauggcaga aguaagaucc uauuguuaug aagcaucgau aagcgauaug gccucagauu      840

cuagaugucc cacacaagga gaagcuuacu uagauaaaca aucugauaca caauaugucu      900

guaaaagaac acucguagau agaggauggg gaaauggaug uggccuauuc ggaaaaggua      960

guuuaguaac augugcaaaa uuugcaugua gcaaaaaaau gacaggaaaa agcauucaac     1020

cagaaaaucu ggaauauaga auaauguuau cuguacaugg aagucaacau ucagggauga     1080

uaguuaauga uacaggacac gaaacagaug aaaaucgagc aaaaguugaa auaacaccua     1140

auaguccaag agcagaagca acauuaggag gauuuggaag ucuaggauua gacugugaac     1200

cuagaacugg auuggauuuu agugacuuau auuaucuuac uaugaauaau aaacacuggc     1260

uuguucauaa agaaugguuc cacgauauac cacuaccaug gcaugcaggc gcugacacag     1320

guacacccca uuggaauaac aaagaagcac uaguagaauu caaagaugcu caugcaaaaa     1380

gacaaacagu cguaguucua ggaagucaag aaggagcagu acacacagcu uuagcuggag     1440

cauuagaagc agaaauggau ggugcaaaag gacgacuauc aucaggacac uuaaaaugca     1500

gacuaaaaau ggauaaauua agacuaaaag gaguaucaua uaguuugugu acagccgcau     1560

uuaccuuuac aaaaauucca gcugaaacau uacacggaac uguaacagug gaaguacaau     1620

augcagguac agacggaccg uguaagguac cagcucagau ggcaguagau augcaaacau     1680

uaacgcccgu ugguagauua auaacugcaa auccagugau aacagaauca acagagaaua     1740

gcaaaaugau gcuagaauua gaucccccau uuggagauag cuauauagua auuggaguag     1800

gagaaaaaaa aauaacacau cauuggcauc gaucaggauc cacuauaggu aaagcuuuug     1860

aagcaacagu aagaggggcu aaaagaaugg cgguauuagg agauacagcc ugggauuuug     1920

gaucagucgg aggugcauua aacaguuuag guaaaggaau ucaucaaaua uuuggagcag     1980

cauucaaaag cuuauuugga gguaugucau gguuuucgca aauacugauu ggaacauugc     2040

uaauguggcu aggacuaaau acuaaaaaug gaaguaucuc auuaaugugu cuagcacuag     2100

gagguguauu aauauuucuc agcacagcag ucucagcaga uugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6953
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6953
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

gaagaggugc agauacauca guaggaauag uuggguuauu acuaacaaca gcaauggcag      120

cagaaguaac gagaaggggu agugcguacu auauguaccu agaccgaaau gaugcaggug      180

aagcuauaag cuuuccaacc acacuaggca ugaauaaaug uuauauacaa auaauggauc      240

uaggacauau gugugaugcu acaaugaguu acgaaugucc aaugcuagac gaaggaguag      300

agccagacga uguagacugu ugguguaaua cgacaucaac auggguagua uaugguacau      360

gucaucacaa aaaaggcgaa gcaagacgau caaggagagc cgucacucua ccaagucaca      420

gcacaagaaa acuacaaacu agaagucaaa caugguuaga aucaagagaa uauacaaaac      480

aucuaauaag gguggaaaac uggauauuca gaaauccagg uuuugcacua gcagcagcag      540

cgauagcuug guuauuaggu aguucuacuu cacaaaaagu uauauacuug guaaugauuu      600

uauuaauagc acccgcauac aguauaaggu gcauuggagu aaguaauaga gauuuuguag      660

agggaaugag uggaggaaca uggguagaua uaguauuaga acauggaggc uguguaacug      720

ugauggcaca agacaaaccu acaguagaca uugaauuagu aacgacaaca guuucaaaua      780

uggcagaagu aagaucuuau uguuaugaag caaguauaag cgauauggca agugacagca      840

gauguccaac acaaggggaa gcuuaucuug acaagcaauc agacacgcaa uacguuugua      900

aacggacauu aguagauagg ggcuggggga acggaugugg auuauuugga aaaggguccu      960

uaguaacaug cgcaaaauuc gccuguagua aaaaaaugac aggaaaaucu auacaaccag     1020

aaaaucucga auauagaaua auguuaucag uacauggaag ucaacauuca gggaugauag     1080

uaaaugauac uggccaugaa acagacgaaa auagagcaaa aguagaaaua acaccuaaua     1140

gucccagagc agaagcaaca uuaggaggau ucggaucauu aggauuagac ugugaaccaa     1200

gaacaggccu agauuucucu gacuuauauu accuaacaau gaauaauaaa cacuggcuag     1260

uucacaaaga gugguuucau gacauaccgc uaccuuggca ugcaggugca gauacaggaa     1320

cgccacauug gaauaauaaa gaagcacuag uagaguuuaa agaugcucau gcaaaaagac     1380

aaacgguagu aguauuagga agccaagaag gagcaguaca cacagcucua gcaggagcuc     1440

uugaagcaga aauggauggu gcuaaagguc gauuaucauc cggacacuua aaauguagau     1500

uaaaaaugga caaacuaaga uuaaaaggag uaagcuacag ccuauguaca gcagcauuua     1560

cauuuacaaa aauaccagca gaaacuuuac auggaacagu aacaguugaa guacaauaug     1620

caggaacaga ugggccaugu aaaguuccug cgcaaauggc aguagacaug caaacauuaa     1680

caccaguagg aagauugaua acagcaaauc caguaauaac ugaaucaacu gaaaacucaa     1740

aaaugaugcu agaacuagac ccaccauucg gagauagcua uauaguaauu ggaguuggcg     1800

aaaaaaaaau aacacaucau uggcacagaa guggaaguac gauuggaaaa gcauuugagg     1860

caacaguaag aggugcuaaa agaauggcag uacuuggaga cacagcuugg gauuuuggua     1920

gcguaggagg agcauuaaau ucacuuggaa aaggaauuca ucaaauauuc ggagcagccu     1980

ucaaaucacu auuuggagga augaguuggu uuucacagau auuaauuggg acacuucuaa     2040

uguggcuagg auugaauaca aaaaauggga guauaucuuu aaugugucua gcauuagggg     2100

guguauuaau auucuuguca acagcugugu cugccgauug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6954
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6954
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

gaggcgcaga uacaucaguc ggaauuguug gucuuuuguu aacuacagca auggcagcag      120

aaguaacaag acguggaagu gcguacuaca uguaccuaga cagaaaugau gcaggagaag      180

caaucucauu uccuacuacu cuggguauga acaaaugcua uauacaaaua auggaucuag      240

gccauaugug ugaugcgaca augucauaug aguguccaau gcuagacgaa gguguugaac      300

cugacgaugu agauugcugg uguaacacaa caucaacaug gguaguauau ggaacaugcc      360

accauaagaa aggagaagcu agaagaucaa gacgagcagu aacauuaccc agucauagua      420

caagaaaauu acagacaaga agccaaacau ggcuagaauc gagagaauau acuaaacauc      480

uaauaagagu agaaaauugg auauuccgaa acccaggauu ugccuuggca gcagcugcca      540

ucgcauggcu uuuaggaagu ucgacaagcc aaaaaguaau auaucuaguu augauacuuc      600

ucauagcacc agcuuauuca auaagaugua ucgguguuag uaacagagau uuuguagaag      660

guauguccgg agguacuugg guugauauag uauuagaaca ugguggaugu guuacuguaa      720

uggcacaaga uaaaccaaca guagauauag aauuaguaac aacuacagua agcaacaugg      780

cagaaguaag aucuuauugu uaugaagcau caauaucaga uauggcaagu gauaguagau      840

gcccaaccca aggagaagca uacuuagaua aacagucaga uacacaauau guauguaaac      900

gaacuuuagu ugaucgaggg uggggaaacg gaugugguuu auuuggaaaa ggaaguuuag      960

uaacaugugc uaaauuugca ugcucaaaga aaaugacagg aaaaucaaua caaccagaaa     1020

auuuagaaua uaggauaaug cuuagcguac auggaucaca acauuccgga augauuguaa     1080

augacacggg acaugaaacu gaugaaaaca gagcuaaagu cgaaauaaca ccuaauaguc     1140

cucgagcaga agcaacauua ggaggauuug guagcuuagg acuugacugu gaaccuagaa     1200

caggacuaga uuuuagugau cuauacuacc uuaccaugaa uaauaaacau uggcuaguac     1260

acaaagaaug guuucaugac auaccauuac cauggcaugc cggagcagac acagguacac     1320

cucacuggaa uaauaaagaa gcccuaguag aauuuaaaga ugcacacgca aaacgacaaa     1380

cagugguggu cuuaggaagc caagaaggag caguacauac agcauuagcc ggugcucuag     1440

aagcagaaau ggauggugca aaaggaagac uuagcucagg acauuuaaaa uguagguuaa     1500

aaauggauaa auuaagguua aaaggaguau cauauagucu augcacagca gcauuuacuu     1560

ucacaaaaau acccgccgaa acgcuacacg gaacaguaac aguagaagua caauacgcag     1620

gaacugaugg uccauguaaa guaccagcuc aaauggcagu agacaugcaa acauuaacac     1680

caguagguag guuaauaacc gcgaauccag uaauaacuga aucaacagaa aauagcaaaa     1740

ugauguuaga acuagacccu ccauucggag auagcuauau aguaauaggu guaggagaga     1800

agaaaaucac acaucauugg cauagaagug gaucaacaau aggaaaagca uuugaagcua     1860

caguaagagg agcaaaacgu auggcaguau ugggagauac ugcaugggau uuuggaagug     1920

uagggggagc ucuaaauuca cuuggcaaag gaauacauca aauauuugga gcagcauuua     1980

aauccuuauu cggaggaaug ucuugguuua gccaaauacu aauaggaacu uuauuaaugu     2040

ggcuuggacu aaacacuaaa aauggaucaa uuucuuuaau gugucuagcu cuaggaggag     2100

uacuuaucuu ucuaaguaca gcagugagug cagacugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6955
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6955
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gagcagacac aaguguaggu auaguuggau uauuauuaac aacagcaaug gcagccgaag      120

uaacaagaag agguucagcu uauuauaugu auuuagacag aaaugaugca ggagaagcaa      180

uaucauuccc aaccacgcuu ggaaugaaua aaugcuauau acaaauaaug gauuuaggac      240

acauguguga ugcaacaaug ucauaugaau gccccauguu agaugaaggu guagaaccag      300

augacguaga cuguuggugu aauacaacga gcacaugggu cguauaugga acuugccauc      360

auaagaaagg agaagcuaga cgaagcagac gagcaguaac gcuaccaagu cacucaacca      420

gaaaauugca aacaaggagu caaacauggc uagaaucaag agaauauacu aaacaucuua      480

ucagaguaga aaauuggauu uuucgaaauc caggauuugc acuugcugca gcugcuauag      540

cauggcuauu agguucaagc accucacaaa aaguaauaua ucuaguuaug auacuauuaa      600

uugccccugc auacucaauu agauguauag gaguaucaaa uagagacuuc guagagggaa      660

ugucaggagg aacuugggua gauauaguac uagaacaugg uggaugugua acaguaaugg      720

cacaagauaa accaacuguu gacauagaau uaguaacaac aaccguaagu aauauggcag      780

aaguaagauc cuacugcuau gaggcaagua uaagcgauau ggcaucagau agucgauguc      840

caacacaagg ggaagcauau uuagauaaac aaucagauac gcaauacguc uguaaaagaa      900

cauuagugga cagaggaugg gguaaugguu guggacuauu cggaaaaggu agcuuaguua      960

caugugcaaa auuugcaugu aguaagaaaa ugacaggaaa aucaauucaa cccgaaaacu     1020

uagaauauag aauaaugcua ucaguacaug gaagucaaca uucagguaug auuguaaacg     1080

auaccggcca ugaaaccgau gaaaauagag cuaaaguaga aauaacgccu aauagcccaa     1140

gagcggaagc aacacuggga ggauuuggga gcuuaggauu ggauugugaa ccaagaacag     1200

gacuagauuu cucugacuua uauuaccuaa ccaugaauaa uaaacauugg uuaguacaua     1260

aagaaugguu ucaugauaua ccacuaccgu ggcaugcagg ggcagauacc ggaaccccac     1320

acuggaauaa uaaagaagcc uuaguagaau uuaaagacgc gcaugcaaaa agacaaacug     1380

uaguaguauu aggaucacaa gaaggggcag uucacaccgc cuuagcagga gcacuagaag     1440

cagaaaugga uggagcuaaa ggucgacuau caucaggaca uuuaaaaugu agacuuaaaa     1500

uggauaaauu aagauuaaaa ggaguuaguu acucauuaug cacugcagca uucacuuuca     1560

ccaaaauacc agcagaaaca uuacauggca cuguaacagu agagguacaa uaugccggaa     1620

cagauggacc cugcaaagua ccagcgcaga uggcugucga uaugcaaaca uuaacuccag     1680

uuggucgacu cauaacugcu aaucccguaa uaacagaauc aacagaaaau ucaaaaauga     1740

ugcuugaauu agauccuccc uuuggagauu cauauauagu aauaggagua ggggaaaaga     1800

aaauaaccca ccauuggcau agaaguggaa guacaauugg uaaagcauuu gaagcaacag     1860

uuagaggagc aaaaagaaug gcgguauuag gagauacagc gugggacuuc ggcagcguag     1920

gaggcgccuu aaauucuuua ggaaaaggga uacaucaaau auuuggagca gcauucaaau     1980

cauuauucgg aggaauguca ugguuuucac aaaucuuaau aggaacucua uuaauguggu     2040

uaggucuaaa uacuaaaaac ggcucaauaa guuuaaugug uuuggcacua ggcggaguac     2100

uaauuuuccu aucgacagca guauccgcag acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6956
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6956
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggag       60

cggauacauc aguugguaua guaggacugu uauuaacaac agcuauggcu gcagaaguaa      120

ccagaagagg aucagcauau uauauguauu uagauagaaa ugaugcugga gaagcuauau      180

cauuucccac caccuuagga augaauaaau guuacauuca aauaauggau uugggacaca      240

ugugcgaugc aacaaugagc uaugaguguc ccauguuaga cgaaggaguu gaaccagaug      300

acguagauug uugguguaau acgacaagua cuuggguagu auacggcaca ugucaucaca      360

agaaaggaga agcacgccgc ucaagacgag cuguaacccu ucccagucau aguacaagga      420

aauugcaaac ucguagccaa acaugguuag aauccagaga auauaccaaa caucucauaa      480

gaguagaaaa cuggauauuu agaaauccag gauucgcauu agcugcagca gcaauagcau      540

ggcuccuagg aucaagcaca ucccaaaaag uuauauaccu aguuaugaua uuauuaauag      600

cuccagcaua uucaauuaga uguauaggag uuucaaaccg ugacuuuguu gaaggcaugu      660

caggaggaac auggguagau auaguacuag aacacggagg auguguaacu guaauggcac      720

aagacaaacc cacaguugau auagagcuag uuacuacaac aguaaguaau auggcagaag      780

uaagaaguua uugcuacgaa gcaucaauaa gugauauggc aagugacagu agaugcccaa      840

cccaaggaga agcguacuua gacaaacaaa gugauaccca auauguuugu aaaagaacac      900

uuguugaucg aggaugggga aauggaugcg gacucuuugg uaaaggauca uuaguaacau      960

gugcaaaauu ugccuguagc aagaaaauga caggaaaauc aauacaacca gaaaaccuag     1020

aauauagaau aauguuaucu guacaugguu cacaacauuc agguaugauu gucaacgaca     1080

caggacauga aacugaugaa aauagagcaa aagucgaaau aacuccaaau ucaccacgag     1140

cagaagccac uuuagggggu uuuggaaguc uaggauuaga cugugaacca aggacaggau     1200

uagauuuuuc agaucuauau uauuuaacaa ugaacaacaa acauuggcua guacacaaag     1260

aaugguuuca ugacauacca uuacccuggc acgcaggcgc ugacacugga acuccgcacu     1320

ggaauaauaa agaagcccua guugaguuca aagacgcaca ugcaaaaaga caaacaguag     1380

uuguauuagg aucacaagaa ggagcuguac auacagcauu ggccggagca uuagaagcug     1440

aaauggaugg agcaaaaggu agacuaagca gcggccaucu caaaugcaga uuaaaaaugg     1500

acaaacuaag acuaaaaggc guuucauacu cacuauguac agcagcuuuu acauuuacua     1560

aaauaccugc agaaacauua caugguacag uaacaguuga aguacaauau gcaggaacag     1620

auggaccaug caaaguacca gcacaaaugg cagucgauau gcagacauua acgccaguag     1680

gacgacuaau aacagcaaau ccuguaauaa cagaaagcac agaaaauuca aaaaugaugc     1740

uagaacucga cccucccuuc ggggauucau auauaguaau aggaguaggc gaaaagaaaa     1800

uaacacacca uuggcaucgu ucaggaagua cuauaggaaa agcauucgaa gccacaguaa     1860

gaggugcaaa aaggauggca guguuaggag auacagcaug ggauuuugga ucagucggag     1920

gagcacuaaa uagcuuagga aaaggaauac aucagauauu uggagcagca uuuaaaaguu     1980

uauucggugg aaugucuugg uucucucaaa uuuuaauagg aaccuuacuu augugguugg     2040

ggcuaaauac caaaaaugga ucaauaucau uaaugugcuu agcucuagga gguguacuaa     2100

uauuucuauc gacagcagug uccgcagauu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6957
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6957
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcug       60

auacaucagu aggcauagua ggucuacuuu uaacaacagc aauggcagcu gaaguaacaa      120

ggcgagguag cgcuuauuac auguacuuag auagaaauga ugcaggggaa gcuauaucuu      180

uuccgacaac auuaggaaug aauaaaugcu auauucaaau aauggaccug ggacauaugu      240

gugaugcgac aaugaguuau gaaugcccaa uguuagauga aggaguugaa ccggaugacg      300

uagacugcug guguaauaca acaaguacau gggucguaua ugguacaugu caucauaaaa      360

aaggagaagc aagaagauca agaagagcag uuacauuacc uucacacucu acaagaaaau      420

ugcaaacacg aagccaaacg uggcuugaau caagagagua cacaaaacau uuaauccgag      480

uagaaaauug gauuuucaga aauccaggau ucgcacuagc cgcagcagca auagcauggu      540

uacucggauc aaguacauca caaaaaguaa uuuacuuagu aaugauauua cuaauagccc      600

cagcauauag uauaagaugu auaggaguaa guaauagaga uuuuguggaa ggaaugagug      660

gagguacgug gguagauauu guauuagaac augguggaug uguaacagua auggcacagg      720

auaaaccaac aguagacauu gaauuaguaa caacaaccgu aucaaacaug gcagaaguaa      780

gaucauacug uuaugaagca aguauaucug auauggcgag cgacucaaga uguccuacac      840

aaggugaagc guaucuagau aaacaauccg auacucaaua uguauguaaa agaacccuag      900

uagacagagg guggggcaau gguugugguu uauuuggcaa aggaucauug guaacaugug      960

caaaauucgc augcucaaaa aaaaugacug gaaaaucaau ucagccggaa aaccuagaau     1020

auagaauaau guuaucuguu cacggaaguc aacacucagg aaugauugua aacgauacug     1080

gucacgaaac ugaugaaaau agagcuaaag uagaaauaac accaaauagu ccaagagcag     1140

aagccacccu ugguggauuc ggaagucuag gauuagauug cgaaccaagg accggauuag     1200

acuucucaga uuuauauuau cuaacuauga auaauaaaca uuggcuagua cacaaagaau     1260

gguuucacga uauaccuuua ccauggcaug caggagcaga uaccgguaca ccacacugga     1320

acaauaaaga agcuuuagua gaauuuaaag augcucacgc aaaacgacaa acgguuguag     1380

uacuaggauc acaagaagga gcaguccaca cagcauuagc gggagccuua gaagcagaaa     1440

uggauggagc aaaaggaaga cuuaguucag gacauuuaaa augccgauua aaaauggaua     1500

agcuuagacu aaaaggagua aguuacagcc ugugcacagc ugcauuuaca uuuacaaaaa     1560

ucccagcgga aacauuacau ggaaccguca caguagaagu acaauacgcc ggaacagaug     1620

gcccaugcaa aguaccagcu caaauggcug uagauaugca aacacuaaca ccaguaggaa     1680

gacuaauaac agccaaccca guaauaaccg agucuacaga aaacaguaaa augauguuag     1740

aacucgaucc uccauuugga gauagcuaua uaguaauugg uguaggagaa aaaaaaauaa     1800

cacaucauug gcaucgauca gguucaacaa uagguaaagc auuugaagcu acaguacgag     1860

gagccaaaag aauggcagua uugggugaua cagcauggga uuuuggaucu gucggaggag     1920

cauuaaacuc auuaggaaaa gguauacauc aaauauuugg ugcugcauuc aaaagcuuau     1980

uuggaggaau gucaugguuu ucccaaauuc uaauaggaac auuauuaaug ugguuaggau     2040

uaaacacgaa aaauggguca auaagucuaa ugugccuugc auuaggugga guacuaauau     2100

uucuaagcac ugcaguauca gcugauugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6958
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6958
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

caaguguagg aauaguagga uuguuauuaa caacugcaau ggcagcagaa gucacucguc      120

gugguagcgc auacuauaug uauuuagaua gaaaugaugc aggagaagca auaucuuuuc      180

cgacaacauu aggaaugaac aaauguuaua uacaaauaau ggauuuaggu cauaugugug      240

acgcuacgau gucauaugaa uguccaaugu uggaugaagg aguagaacca gaugacgucg      300

auuguuggug caauacgaca uccacauggg uaguuuaugg gacaugucau cauaagaaag      360

gagaagcgag gagaagcagg agagcaguaa ccuuaccaag ucacagcaca agaaaacuac      420

aaacaagaag ucaaacaugg uuagaaucaa gagaauauac caaacacuua auaagggugg      480

aaaauuggau auuucgaaau ccaggauuug cauuagcagc agcugcaauc gcgugguuau      540

uaggaucauc aacaucacag aaaguaauau accuaguuau gauauuauua auagcaccag      600

cauauagcau aagaugcaua ggagugagua auagagauuu uguagaagga augaguggag      660

gcaccugggu agauauagua cuagaacaug gcggaugugu caccguuaug gcucaagaua      720

aaccaacagu agauauagaa uuaguaacua caacaguauc aaauauggca gaaguaagaa      780

guuacugcua ugaggcuuca auaagugaca uggcuucaga uucaagaugu ccgacacaag      840

gagaagcaua uuuagauaaa cagucugaua cucaauaugu augcaaaaga acccuaguag      900

acagaggaug gggaaauggu ugugguuuau ucggaaaagg aagucugguu acaugugcaa      960

aauucgcaug cucuaagaaa augacaggaa aaucaaucca accagaaaac cuagaauaca     1020

gaauaauguu aucaguacac ggcucacaac auaguggaau gauagugaau gauacuggac     1080

acgaaacuga ugaaaaucga gcaaaaguag aaauaacacc caacaguccg agagcagaag     1140

cuacauuagg aggauuuggu ucauuaggau uggauugcga accaagaaca gguuuagacu     1200

uuagugauuu auauuaucuu accaugaaua acaaacauug gcuuguacau aaagaauggu     1260

uucaugacau uccucuacca uggcaugccg gagcagauac ggguacucca cacuggaaua     1320

auaaagaagc auugguagaa uuuaaagaug cacaugcaaa aagacaaacu guaguaguau     1380

uaggaaguca agaaggagca guucauacug cguuagcugg agcauuagaa gccgaaaugg     1440

auggagcaaa gggaagauua ucaaguggac accuuaaaug ccgacugaaa auggauaaac     1500

uaagacuuaa agguguuucu uauucuuuau guacugcggc uuucacauuu acgaaaauac     1560

ccgcugaaac acuucauggu acuguaacag uagaaguaca auaugcagga acagacggac     1620

cauguaaagu accagcacaa auggcagucg auaugcaaac acuuacacca guagguagac     1680

uuaucacagc gaauccaguu auaacugaaa guacagaaaa uaguaaaaug augcuggaau     1740

uagauccucc uuucggagac aguuacauag uuauuggugu aggggaaaag aaaauaacac     1800

aucacuggca uagaagugga ucaacuauag gaaaagcauu ugaagcaaca guccguggag     1860

caaaacgaau ggcaguacua ggcgauacug caugggauuu ugguagcguu gguggagcac     1920

uaaauucacu uggaaaagga auacaccaaa uauuuggagc agcauuuaaa ucacuuuuug     1980

guggcauguc augguuuuca caaauuuuaa uuggaacauu auuaaugugg cuaggauuaa     2040

auacuaaaaa cgguaguaua ucguuaaugu gucuagcacu agguggaguc uuaauauuuc     2100

uuaguacagc aguuagugca gauugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  6959
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6959
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gugucggaau aguaggauua uuguuaacaa cggcaauggc cgcagaagug acgagaaggg      120

ggucugcuua uuauauguau cuugauagaa augaugcagg agaagcuaua aguuuuccaa      180

caacucuagg aaugaauaaa uguuacauuc agauaaugga cuuagggcau augugcgaug      240

caacaauguc auacgagugc ccaaugcuag augaaggagu agaaccagau gacguugacu      300

gcugguguaa uacaacuuca acauggguag ucuacgguac augucaucac aaaaaaggag      360

aagcaagaag aucuagaagg gcagugacau uaccuaguca uagcacgagg aaacuccaaa      420

cccgaucaca aacauggcua gaaagcagag aauauacaaa gcaccuaaua agaguagaaa      480

auuggauuuu uagaaaucca ggauucgcau uagcagccgc ugcgauugca ugguuacuug      540

gaucaaguac gucgcaaaaa guuauauacu uaguaaugau auugcuaauc gcaccagcau      600

auucgaucag augcauagga guaucaaaca gagacuucgu agaaggaaug aguggaggaa      660

cauggguaga uauaguacua gaacauggag guugugugac aguaauggca caagauaaac      720

caacugugga cauagaacua gugacaacaa caguaucaaa cauggcagaa guacgaucuu      780

acuguuauga agcgucaaua agcgauaugg caagcgauag uagaugccca acacaaggag      840

aagcauacuu agauaaacaa ucagauacuc aauauguaug uaaacgaaca cuaguagaua      900

gaggaugggg aaauggaugu ggauuauuug gcaaaggaag ucugguaacu ugugcaaaau      960

uugcuugcag uaaaaaaaug acaggaaaaa guauacaacc agaaaaccuu gaauauagga     1020

uuauguuaag uguucacggg agucaacaua gugggaugau ugucaaugau acaggacaug     1080

aaacagacga aaauagagca aaagucgaaa uaacgccaaa caguccaaga gcugaagcga     1140

cauuaggagg auuuggauca uuaggacuag auugugaacc aagaacuggg cuagacuuuu     1200

cagacuuaua cuaucuaaca augaauaaua agcacugguu aguucauaaa gaaugguuuc     1260

augacauacc acuaccuugg caugcaggag cagauacugg aacuccgcau uggaauaaua     1320

aagaagcauu aguagaauuc aaagaugcac augcaaaacg acagacagua gucguacuug     1380

gaagucaaga aggagcggug cauacagccu uagcaggagc auuagaagca gagauggacg     1440

gcgcuaaagg uagacuuagc agcggacauc uaaaauguag acuaaaaaug gauaaacuaa     1500

gacuaaaagg aguaucauau ucauuaugua ccgcagcguu cacauuuaca aaaauaccag     1560

cggaaacauu acauggaacu guaacaguag aaguacaaua ugcaggaacu gauggaccau     1620

guaaaguacc agcacaaaug gcaguagaua ugcaaacauu aacacccgua ggaagacuaa     1680

uaacagcaaa uccaguaaua acagaaagua cagaaaauuc uaaaaugaug uuggaauuag     1740

acccaccuuu uggagacagu uacauaguaa uaggaguagg agaaaaaaaa auaacacacc     1800

auuggcauag gaguggaagc accauaggaa aagcauuuga agcuacugua agaggagcga     1860

aacgaauggc uguucuagga gauacggcuu gggacuuugg aaguguagga ggagcguuaa     1920

auagucucgg caaaggaauu caucaaauau ucggagcagc auuuaaauca cuauuuggug     1980

gaaugucaug guuuagucaa auauugauag gaacacuauu aaugugguua ggauuaaaua     2040

cuaaaaacgg caguauauca cuaaugugcu uagcauuagg uggaguuuua auauuccuaa     2100

guacagcagu aagugcagau ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6960
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6960
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau uguugguuua cuauuaacaa cagcaauggc agccgcagua acacgccgag      120

gcucggcuua cuauauguau uuagauagaa acgaugcagg agaagcaaua ucauuuccaa      180

ccacacuggg uaugaauaaa ugcuauaucc aaauaaugga ucuaggacau augugcgacg      240

caacaauguc auaugaaugu ccaaugcuag augaaggagu cgaaccagau gauguagauu      300

gcugguguaa uaccacaagu accuggguag uauauggcac augucaucau aagaaaggcg      360

aagcaagaag aaguagacga gcaguaacau uaccaucaca uucaacaaga aaacuucaaa      420

caagaagcca aacuuggcua gaaagcagag aauauacaaa acauuuaaua agaguggaaa      480

auuggauauu cagaaauccu ggcuuugcau uagcugcagc ggcuauagcu ugguuacuug      540

gaucuaguac uucacagaaa guaauauauc uaguaaugau acuauuaaua gcaccagcuu      600

acaguauccg augcauagga guaucgaaca gagauuuugu agaagggaug ucagguggaa      660

cauggguaga uauaguauua gaacacggcg gaugcguaac aguaauggca caagacaaac      720

caacaguaga cauagaacuu guaacaacaa caguaucaaa uauggcagaa guuagaucau      780

acugcuauga agcaucaaua ucagacaugg cuucagauag uagaugucca acacaaggag      840

aagccuacuu agauaaacag uccgauacuc aauauguaug uaaaagaacc cuaguagacc      900

gcgguugggg aaaugguugc ggacuauucg gaaaaggaag ucuaguaacc ugugcaaaau      960

uugcauguag uaagaaaaug accggaaaaa guauucaacc agaaaauuua gaauauagaa     1020

uaauguuaag uguacauggc ucucaacauu caggaaugau agucaaugau acaggucaug     1080

aaacagauga aaauagagcu aaagucgaaa uaacaccaaa uucaccacgu gcugaagcaa     1140

cacuaggagg auuuggauca cucggauuag auugugaacc aaggacaggc uuggauuucu     1200

cagauuuaua uuaucuaacu augaacaaca aacauugguu aguccauaaa gaaugguuuc     1260

acgauauacc ucuaccaugg caugcuggug cagauacagg aacaccccau uggaauaaua     1320

aagaagcucu agucgaauuu aaagaugcac acgcaaaacg ccagacagua guuguacuag     1380

gaagccaaga aggagcagua cacacagcuc uagcaggagc uuuagaagca gaaauggaug     1440

gugcaaaagg aagacuaagc ucaggacauc ugaaaugcag gcuaaaaaug gauaaauuaa     1500

gauuaaaagg aguaagcuau ucauuaugua cagcagcauu uacauucaca aagauaccag     1560

cggaaacauu acacgggacc guaaccguag aaguacaaua ugcagguaca gacggaccau     1620

guaaaguacc agcacaaaug gcaguagaca ugcaaaccuu aacaccagua ggucgauuaa     1680

uaacagccaa uccaguaaua acagaaucaa ccgaaaauuc caaaaugaug cuagaauuag     1740

auccaccuuu cggagauagu uauauaguaa uaggaguagg agaaaagaaa aucacacauc     1800

acuggcauag aucuggcucc acuauaggaa aagcauuuga agcuaccguu agaggagcaa     1860

aaagaauggc ugucuuagga gauacugcau gggacuuugg aaguguugga ggugcacuua     1920

acagccuagg aaaaggaaua caucaaauau ucggugcagc uuuuaaaagu cucuucggug     1980

gaauguccug guucucucaa auuuuaauag guacacuacu uauguggcuu ggucuaaaca     2040

ccaaaaaugg aucaauauca uuaaugugcc uggcacuagg aggaguacua auauucuuau     2100

caacagcugu aagcgcagau ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6961
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6961
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gagcagauac aaguguuggu auaguaggcu uacuauuaac aacagcaaug gccgcugcug      120

uaacacgaag aggaagugca uauuacaugu acuuagaccg aaacgaugca ggagaagcca      180

uaucauuucc aacaacguua ggaaugaaca aaugcuacau acagauaaug gaucuaggac      240

auauguguga cgcaacgaug ucauacgaau guccaauguu agaugaaggc guagaaccag      300

augauguaga uuguuggugc aacacaacaa guacaugggu aguauauggc acaugucacc      360

auaaaaaagg ggaagccaga agaagcagaa gagcgguaac acuaccuuca cauaguacua      420

gaaaacuuca aacgcgcucu caaacauggc uugagaguag agaguauaca aaacauuuaa      480

uuagaguaga aaauuggaua uuucgaaauc ccgguuuugc auuagcagca gcagcaauag      540

cauggcugcu cggaucauca acuucacaaa aaguuauaua uuuaguaaug auauuacuaa      600

uagcaccagc auacagcaua cgaugcauag gaguuucaaa cagagauuuu guagaaggaa      660

ugucuggagg aaccugggua gacauugugc uugagcaugg aggaugcgua acuguuaugg      720

cacaggacaa accaacgguu gauaucgaau uaguuacaac uacaguauca aauauggcgg      780

agguuagauc cuauuguuau gaggcaucua uaucagauau ggcaucagau ucgagauguc      840

cuacucaagg ugaagcuuau cuagacaaac aaagugacac gcaauauguu uguaaaagaa      900

cuuuaguuga cagaggaugg ggaaauggcu gugguuuauu uggaaaaggg aguuuaguaa      960

caugugcaaa auuugccugu ucuaaaaaaa ugacaggcaa aucaauacaa ccagaaaauu     1020

uagaauauag aauaauguua ucaguacaug gaagucaaca uagcggaaug auaguuaaug     1080

auacaggaca cgaaaccgau gaaaaccgug cuaaaguuga aauaacacca aauucaccaa     1140

gagcagaagc aacauuagga ggauucggau ccuuaggacu ugacugugaa ccaagaacag     1200

gacuagacuu uagugacuua uauuauuuga caaugaauaa uaaacauugg uuaguacaua     1260

aagaaugguu ucacgauaua ccauuaccuu ggcacgcagg agcagauaca ggcacaccac     1320

auuggaauaa caaagaagcu uuaguagaau ucaaagacgc acacgcaaaa cgacaaacug     1380

uugucguacu aggaucacaa gagggugcag uacacacagc gcuagcugga gcauuagaag     1440

cagaaaugga cggagcuaaa ggacgcuuau caucagggca ccuaaaaugu cgacuaaaaa     1500

uggauaaacu aagguuaaaa ggaguauccu auucccuaug uacagcagca uucacauuca     1560

caaaaauacc agcagaaaca uuacacggaa caguuacugu ggaaguacaa uaugcaggaa     1620

cagauggacc auguaaaguu ccagcucaaa uggccguaga caugcaaacg uuaacaccag     1680

uaggaagacu uauuacagcg aacccaguua uaacagaaag uacugaaaau aguaaaauga     1740

uguuggaauu agauccucca uuuggagauu cuuauauagu aaucggcgua ggggaaaaaa     1800

agauaacaca ucauuggcac agaagcggau cuacaaucgg aaaagcauuu gaagcaacag     1860

uaagaggcgc aaaaagaaug gcaguacuag gagauaccgc augggauuuu gguaguguag     1920

gaggagcacu aaauagccua ggaaaaggaa uacaccaaau auucggggca gcauucaaaa     1980

gcuuauuugg uggaauguca ugguucagcc aaauacuaau aggaacauua uuaauguggc     2040

uuggacucaa caccaaaaau ggaucuauua gccuaaugug cuuagcguua ggaggaguac     2100

uaauauucuu aucaacagca guaagugcag auugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6962
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6962
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gauuaucauc aaggaaacga agaagccaug acgucuuaac uguacaauuc uuaauacuag      120

gaaugcuauu aaugacaggc gguguaacaa gaagaggaag ugcauacuac auguaucuug      180

acagaaacga ugcgggugaa gccauaagcu uuccaacaac cuuaggaaug aauaaauguu      240

acauacaaau uauggacuua gggcauaugu gcgaugcaac aaugaguuau gaauguccua      300

ugcuagauga gggaguagaa ccugacgaug uagacuguug gugcaacaca acaucaacuu      360

gggucguuua ugggacuugu caccauaaaa aaggugaggc aagaagaagu agaagagcag      420

uaacauuacc uucacauucc acaagaaaau ugcaaacacg aucacaaaca ugguuagaaa      480

guagagaaua cacaaaacac cuuauaagag ucgaaaauug gauauuucga aauccaggcu      540

uugccuuggc ugcugcagca auugccuggc uauuagguag uucuacuagu caaaaaguga      600

uauauuuagu gaugauuuua uugauagcac cggcauauag cauaagaugu auuggcguaa      660

guaauagaga uuuuguugaa gguaugagug gugguaccug gguagauaua guccuagaac      720

auggaggaug uguaacagua auggcacaag auaaaccaac aguagauauc gaacuaguaa      780

cuacaacugu cucaaacaug gcagagguua gaaguuauug uuacgaagca aguauaagug      840

auauggcaag cgauucucga uguccaacac aaggugaagc uuacuuagau aaacaaucag      900

auacacaaua uguauguaaa agaacacuag uugacagagg augggguaau ggauguggac      960

uauuuggaaa aggauccuua gucacuugcg cuaaauuugc cuguagcaaa aaaaugacgg     1020

guaaaucaau acaacccgaa aauuuagaau accgaauuau guugaguguu cauggaucuc     1080

aacauucagg uaugauugua aaugauacag gacaugaaac agaugaaaac cgagcaaaag     1140

uagaaaucac uccaaacagc ccuagagcag aggcaacccu uggaggauuc ggaaguuuag     1200

gccuagauug ugaaccaaga acaggauuag auuuuaguga cuuauacuac cuaacaauga     1260

auaauaaaca uugguuagua cauaaagaau gguuucauga uauacccuua ccuuggcaug     1320

cuggagcaga cacaggaacg ccacauugga auaacaaaga agcacuaguu gaauuuaaag     1380

augcacaugc aaaaagacaa acaguaguag uauuaggaag ucaagaggga gcagugcaca     1440

cagcuuuagc aggcgcacua gaagcugaaa uggacggugc aaaagguaga cuaucaagug     1500

gacacuuaaa auguagacua aaaauggaca aauuaagauu aaaaggggug ucauauucuu     1560

uauguacagc ugcauuuaca uucacuaaaa uuccagcaga aacacuacau ggaacaguaa     1620

caguggaagu acaauaugca ggaacagaug gaccaugcaa agugccugca caaauggcag     1680

uagauaugca aaccuuaacc ccaguaggaa gauuaauaac ggcaaacccg guaauaaccg     1740

aaucaacuga aaacaguaaa augauguugg aacuagaucc accauuugga gauucauaua     1800

uaguuauagg agugggagaa aaaaaaauaa cccaucauug gcauagaagu ggaagcacaa     1860

uaggaaaggc auucgaagcu acagucagag gagcaaaaag aauggcuguu cuuggagaua     1920

cagcauggga uuuugguagu guugguggag cuuuaaauag ccuuggaaaa ggaauucauc     1980

aaauauuugg agcagcauuc aaaagcuuau uuggaggaau gucuugguuu ucacaaauau     2040

ugauaggaac acuuuuaaug ugguuaggau ugaacacaaa gaacggaagu auaucuuuaa     2100

uguguuuagc auuagguggu guucuaauuu uucuaucaac cgcaguaagu gcagauugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6963
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6963
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gauugaguag uagaaaacga cgaagucacg auguucuaac aguacaauuu cuaauuuuag      120

guaugcuauu gaugacagga gcaguaacaa gacgaggauc agcauauuau auguauuuag      180

auagaaauga ugcaggagaa gcaauaaguu uuccaacuac uuuaggaaug aacaaauguu      240

auauucaaau aauggauuua ggccauaugu gugaugcuac aaugaguuau gaaugcccaa      300

uguuagacga aggaguagaa ccagacgacg uagauuguug guguaauaca acaucaacau      360

ggguagugua uggcacaugu caucacaaga aaggagaagc gagaagaagu agacgagcag      420

uuacacuacc aagucauagc acgagaaaau uacaaacaag aucacagacc ugguuagaau      480

caagagaaua cacuaaacac cugauaagag uagaaaauug gauuuuuaga aaucccggau      540

uugcacuagc agcugcagcu auagcuuggc uauuagguag uuccacuagc caaaaaguaa      600

uuuaccuagu aaugauauua cuaauagcac cagcauauag uauaagaugc auaggagugu      660

caaauagaga uuuuguagaa ggaaugagcg gugguacaug gguggauaua gucuuagaac      720

auggaggaug uguaaccgua auggcacaag acaaaccaac aguugauauc gaacuuguaa      780

caacaacggu aucaaauaug gcugaaguaa gaucauacug uuaugaagca aguauaucag      840

auauggcaag ugacucaaga ugcccaacac aaggagaagc auaucuagau aaacaaucag      900

acacacaaua ugugugcaaa agaacauuag uagacagagg auggggaaau ggauguggac      960

uauucggaaa agggagcuua guaacaugcg cuaaauucgc gugcaguaag aaaaugacag     1020

gaaaaaguau acaaccagaa aauuuagaau auagaauaau gcuaucagua cacggaucgc     1080

aacauucagg uaugauagua aacgauacag gacaugaaac agaugaaaac cgagcuaaag     1140

uggaaauaac gccaaauuca cccagagccg aagcaacauu aggggguuuc ggaaguuugg     1200

gauuggauug cgaaccuaga acgggguuag auuuuucaga uuuauauuac cuaacaauga     1260

auaauaaaca uuggcucgua cacaaagaau gguuucauga uauaccuuua ccauggcaug     1320

caggagccga uacugguacu ccacauugga auaauaaaga agcccuagua gaauuuaagg     1380

augcucacgc aaaaagacaa acaguugugg uucuaggaag ucaagaagga gcgguacaua     1440

cagcauuagc aggcgcauua gaagcugaaa uggauggagc gaaaggucga cugaguucag     1500

gacaucuaaa auguagauua aagauggaua aacuaagacu aaaaggagua ucauauucau     1560

uauguacugc agcuuuuacg uuuaccaaga uuccagcaga aacacuacau ggaacuguua     1620

caguagaagu ucaauaugcu ggaacagacg gaccaugcaa aguacccgca cagauggcug     1680

uagacaugca gacauuaacc ccaguaggac gacuaauaac ggcuaaccca gucaucacug     1740

aaucaacuga aaauucgaaa augaugcuag aacuagaccc accuuuugga gacaguuaua     1800

uaguuauugg aguuggagaa aagaaaauaa cacaucacug gcauagaagc ggaaguacaa     1860

uagguaaagc auuugaagca acaguacgcg gugcaaaaag aauggcaguu uuaggagaua     1920

cugcauggga uuucggaucu guagguggag cacuaaacuc ucuuggaaaa gguauucauc     1980

aaauauuugg agccgcuuuu aaaaguuugu uuggaggaau gagcugguuu ucacagauau     2040

uaaucggaac cuuacuaaug uggcuagguc uaaauaccaa aaauggauca auaaguuuaa     2100

uguguuuagc acuuggagga guauuaauau uccugagcac agcaguaagu gcggacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6964
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6964
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaacgc cagaaaagaa aaaaagagaa gaggggcaga uacaucugug gguaucguag      120

gauuauuacu uacaaccgcu auggcggcgg aaguaacaag aagaggauca gccuacuaua      180

uguaccuaga cagaaaugau gcuggugaag caauaucuuu cccuacaacu uuaggaauga      240

auaaauguua cauccaaaua auggacuuag gacauaugug cgaugcaacu augucguaug      300

aauguccuau guuagaugaa ggaguagaac cggaugacgu ugauugcugg uguaacacaa      360

caagcaccug gguaguauac gggacaugcc aucauaaaaa aggggaagca agacgaagcc      420

gaagagcugu uacuuuacca agucauucaa cgagaaaacu ucaaacgaga ucucaaacau      480

ggcuugaauc cagagaauau acaaaacauu uaaucagggu agaaaacugg auuuuuagaa      540

auccaggauu cgcauuagca gccgcggcga uagcauggcu gcuaggguca agcacaucuc      600

aaaaaguaau auaucuagua augauacuau uaauagcccc agcauauuca auaagaugua      660

uaggaguaag uaacagagau uuuguagaag gaaugucugg uggaaccugg guagauauag      720

uauuagaaca uggaggaugu guaacuguca uggcacaaga uaaacccacc guagacauag      780

aacugguaac aacaacagua ucaaauaugg cugaaguacg uaguuauugc uaugaagcau      840

caauaucaga uauggccuca gauucacgau guccuaccca aggagaagcc uacuuagaua      900

aacaauccga cacacaauac guguguaaaa gaacauuagu cgauagaggc uggggaaaug      960

gcuguggauu auuuggaaaa ggaucacuag uaacaugugc aaaauucgcg ugcucaaaaa     1020

aaaugacagg aaaauccauu caaccugaaa acuuagaaua uagaauuaug uuaaguguac     1080

acgggucaca acauagcgga augauaguaa acgacacagg acaugaaaca gacgagaaca     1140

gagccaaagu agaaauaaca ccaaauagcc caagagcuga agcaacacua gguggauucg     1200

gaaguuuagg auuagacugu gagccacgaa cugguuuaga cuucagcgau cuguacuauu     1260

uaacaaugaa caauaaacac uggcuaguac auaaagaaug guuccaugau auaccauugc     1320

cauggcaugc aggagcagau acaggaacac cacauuggaa uaauaaagaa gcauuaguag     1380

aauuuaaaga ugcacacgca aaaagacaga cgguaguagu uuuaggauca caagaaggag     1440

cuguccauac agcucucgcg ggcgcacuug aagcugaaau ggacggagcg aaaggcagac     1500

uaucauccgg ucacuuaaaa uguagacuaa aaauggauaa acuaagacuu aaaggaguau     1560

cauauucauu auguacugcu gcauuuacau uuacuaaaau accagcagaa acacuucaug     1620

gaacgguaac uguagaagua caauaugcug ggacagaugg accaugcaaa guaccagcac     1680

aaauggcagu ugacaugcaa acauugacac caguuggaag auuaauuaca gcaaauccug     1740

uaauaacaga aagcacagag aauucaaaaa ugauguuaga auuagaucca ccguucggag     1800

auagcuauau aguaauugga guaggagaaa aaaaaauaac acaucauugg cacagaucag     1860

gaucaacuau aggcaaagcu uuugaagcaa caguuagggg cgcaaaaagg auggcaguau     1920

uaggagauac agcaugggac uuuggaagcg uaggaggugc cuuaaauagu cuuggaaaag     1980

gaauacauca aauuuuugga gcagcauuua aaagucuauu ugguggaaug aguugguuua     2040

gucaaauucu aauaggaacu cuauuaaugu ggcuaggacu aaauacaaaa aacgggucaa     2100

uaucauugau gugucuugca uuaggaggag ugcuaauauu cuuaaguaca gccguaagcg     2160

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  6965
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6965
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

acgcaagaaa ggaaaaaaaa agaagaggug ccgauacauc aguaggaauu guugguuugu      120

uguuaacaac agcaauggca gcagaaguua cgagaagagg aucagcauac uacauguauc      180

uugaccgaaa cgaugcaggc gaagcaauaa gcuuucccac aacauuagga augaauaaau      240

guuauauuca gauaauggau uuaggacaua ugugcgaugc uacgaugagu uaugaauguc      300

ccaugcuaga ugaaggaguu gaaccagaug auguggauug uugguguaau acaacaucua      360

cguggguagu auacggaaca ugccaucaua aaaaaggcga agcaagaaga ucaagacgag      420

cgguaacauu accuucacau ucaacuagaa aacuccaaac gagaagccag acauggcugg      480

aaucaagaga auauacaaaa caucuuauua gaguagaaaa cuggauauuu cgaaauccag      540

guuucgcuuu agcagcagca gcaauagcau ggcuacuagg aucuucuaca ucacaaaaag      600

uaauauauuu aguaaugauu uuguuaauag caccagcaua caguauaagg uguauaggug      660

uaaguaauag agacuuugua gaaggaauga gcggaggaac guggguagac auaguauuag      720

aacauggagg auguguuaca guaauggcac aggauaaacc uaccguagac auagaauugg      780

ucacaacuac agucagcaac auggcugaag uacgaaguua uugcuaugaa gcuucaauua      840

gugauauggc aucagauagc agguguccaa cacaagguga agcauauuua gauaaacaaa      900

gcgauacaca auauguaugu aaaagaacau uaguagacag aggauggggg aaugguugug      960

gacuauuugg uaaaggaagc uuaguaacau gcgcuaaauu ugcaugcuca aaaaaaauga     1020

cuggcaaaag cauacaacca gaaaauuuag aguaucgaau aauguuauca gugcacggaa     1080

gucaacacuc aggaaugauc gugaaugaca caggccauga aacggaugaa aacagagcaa     1140

aaguagaaau aacuccaaac ucaccaagag cagaagcaac acuaggagga uuuggaucau     1200

uaggacuaga cugcgaaccu agaacaggac uagauuucuc agauuuauac uaucuuacaa     1260

ugaauaacaa acauugguua guccauaaag aaugguuuca ugauauucca uuaccauggc     1320

augcaggagc ugauacagga acgccccauu ggaauaauaa ggaagcuuua guagaauuua     1380

aagaugcaca cgcaaaaaga caaacaguag uuguauuagg gagucaagag ggugcaguac     1440

acacagcauu agcuggugcg cuagaagcag aaauggacgg ggcaaaagga agacuuaguu     1500

cuggacauuu aaaaugcaga cuaaaaaugg auaaauuaag auuaaaaggc guaaguuaua     1560

guuuaugcac ugcugcauuc acauuuacaa aaauaccagc agaaacauua cauggaacgg     1620

uaacaguaga aguacaauau gcaggaacug auggaccaug caaagugccc gcacaaaugg     1680

caguggauau gcaaacgcuc acaccaguag gaagauuaau aacagcuaau ccagucauaa     1740

cagaaucaac ugaaaauuca aagaugaugu uagaauuaga cccaccuuuu ggagauucau     1800

acauuguaau aggaguagga gaaaaaaaaa uaacacauca uuggcauaga aguggaucaa     1860

caauaggaaa agcauuugaa gcaacaguac gaggggccaa aagaauggca guacuaggag     1920

auacagcaug ggacuuugga ucgguagggg gagcucuaaa uuccuuagga aaaggcauac     1980

aucaaauauu uggagcggcg uucaaaucuu uauuuggcgg uaugucuugg uucucucaaa     2040

uuuuaauagg aacccuucua augugguuag guuuaaauac uaaaaaugga ucuaucagcu     2100

uaaugugcuu agcccuagga gguguauuaa uuuuuuuauc aacagcaguu ucagcuugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  6966
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6966
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

uaaaugcuag gaaagaaaaa aaaagacggg gagcagauac aaguguagga auaguaggau      120

uacuacuaac aaccgccaug gcagcagaag ucacaagaag agguucagca uacuauaugu      180

acuuagacag gaacgaugca ggagaagcca uuaguuuucc gacaacacua gguaugaaca      240

aauguuauau acaaauaaug gauuuaggac auaugugcga ugcaacaaug ucauaugaau      300

gcccaauguu agacgaagga guagaaccag augaugucga uuguuggugc aauacaacau      360

caacaugggu aguauacggu acgugucauc acaaaaaagg cgaagcacgu agaagucgaa      420

gagcaguaac ucuaccaagc cauaguacaa gaaaacuaca aacucgauca caaacguggu      480

uagaaagcag ggaauauacu aaacacuuaa uaagaguaga aaacuggaua uuucgaaauc      540

cagguuuugc uuuagcugca gcagcaauag cauggcuauu agggucaucc acgucacaaa      600

aaguaauaua uuuaguaaug auauuacuaa uagcaccagc guacuccaua agauguauag      660

gaguuagcaa uagagacuuc guugaaggaa ugucuggugg aacauggguu gauauagucc      720

uugaacacgg aggaugugua acggucaugg cacaagauaa accaacuguu gauauagaac      780

uaguaaccac aacaguaagc aauauggcag aagucagauc auauuguuac gaagccagua      840

uaagcgauau ggcaucagau ucaagauguc caacucaggg ugaagcauau uuggauaaac      900

aaucagauac acaauauguc ugcaaacgca ccuuaguaga uagagggugg gguaaugguu      960

guggacuauu uggaaaagga ucacuaguaa caugcgcaaa auucgcaugu ucaaaaaaaa     1020

ugacagggaa auccauacaa ccagaaaauu uagaauacag aauaaugcua ucaguccaug     1080

gaucacaaca uagugguaug auuguuaaug auacagguca cgaaacagau gaaaauagag     1140

caaaaguaga aauaacacca aauaguccaa gagcagaagc aacauuggga ggauuuggau     1200

cucuaggucu agauugugaa ccuagaacag gacuggacuu uagugaccuu uauuacuuaa     1260

caaugaacaa uaaacauugg cuaguacaua aagagugguu ccaugauaua ccacuaccau     1320

ggcaugccgg agcagauaca ggaacucccc acuggaauaa uaaagaagcu cuaguagaau     1380

uuaaagacgc acaugccaaa agacaaacag uagucguauu aggaucccaa gaaggagccg     1440

uacauacagc acuagccgga gcauuagaag cagaaaugga uggagcaaaa ggacgacuga     1500

gcucagguca ucuaaaaugu agauuaaaaa uggacaaauu aagauuaaaa ggggugucau     1560

acagucuaug uacagccgca uuuacuuuca caaaaauacc agcugaaaca uuacacggca     1620

cagugaccgu ugaaguacaa uaugcaggaa cagauggucc augcaaagua cccgcacaaa     1680

uggcagucga uaugcaaaca uuaacaccag uaggaagauu aaucacagca aauccaguaa     1740

uaacagaaag uaccgaaaau ucaaaaauga uguuggaauu agauccacca uuuggagaua     1800

guuauauagu aauaggaguu ggagaaaaaa aaaucaccca ccacuggcac agaaguggca     1860

guacaauagg uaaagcguuc gaagcaacug ucagaggagc aaaaagaaug gccguacuug     1920

gagacacagc augggauuuu ggaucaguug gcggcgcucu gaacucccua ggaaaaggaa     1980

uccaucaaau auuuggagca gcauuuaaaa guuuauuugg agguaugagu ugguucucac     2040

aaauacuaau uggaacacuu cuaauguggc uaggacuaaa uacaaagaac ggcaguauau     2100

cauugaugug uuuagcgcua ggaggcguac uaaucuuucu aagcacagcg guuucagcuu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  6967
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6967
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaug       60

caagaaaaga aaagaaaaga agaggugccg auacauccgu agguauaguu ggcuuauuau      120

uaacaacagc cauggcagcu gaagugacua gaagagguuc agcauauuac auguaucuag      180

auagaaauga cgcaggagaa gcaauaaguu uucccacuac auuaggaaug aauaaauguu      240

auauacaaau aauggaucuu ggacacaugu gcgaugcuac aaugaguuau gaauguccaa      300

uguuagauga aggaguggaa ccagaugaug uagauuguug guguaacacg acaucaacuu      360

ggguaguuua uggaacaugu caucauaaga aaggagaagc aagacgauca agacgagcag      420

uaacacuccc aagccacucu acaagaaaau uacagacaag aucacaaaca ugguuagaau      480

cccgagaaua cacaaaacac cuaauuagag uagagaacug gauuuuuagg aauccaggau      540

uugcauuagc ggccgcugca auagcauggc uacuaggauc aaguacaagu caaaaaguca      600

ucuauuuagu aaugauauua uuaauagccc cugcauacuc aauaagaugu auuggcguaa      660

guaauagaga cuuuguagaa gggaugagug gaggaacuug gguagauaua guauuagagc      720

auggaggaug uguaacagua auggcacaag acaaaccaac uguagauauu gaguuaguaa      780

caacaacugu gaguaacaug gcagaaguaa gauccuauug cuacgaagca uccauuucag      840

auauggcuuc agauaguaga uguccaacuc aaggagaagc cuaucuagac aaacaaagug      900

acacacaaua uguuuguaaa agaacauuag uagauagagg augggguaau ggauguggcu      960

uauucggaaa agguucauua guuacaugcg caaaauuugc uuguucaaag aaaaugaccg     1020

gcaaaucaau ucaacccgaa aauuuagaau auagaauaau gcucagugua cacggaagcc     1080

aacauucagg uaugauagua aacgauacug ggcaugaaac agacgaaaau agagcuaaag     1140

uagaaauuac accaaauucc ccaagagcag aagcuacauu gggaggauuu gggagucuag     1200

gacuugauug ugaaccaaga acaggucuag acuucaguga ucuguacuau cuuacaauga     1260

auaacaaaca uuggcuuguc cauaaagaau gguuucacga cauaccacua ccauggcaug     1320

caggagcaga uacuggaacg ccacacugga auaacaaaga agcauuaguu gaauuuaaag     1380

augcacaugc gaaaagacaa acaguaguag uuuuagguag ccaagaagga gcuguacaua     1440

cagcauuagc aggagcauua gaagcagaaa uggauggagc gaaagguaga uuaucaucag     1500

gacaucuaaa augucgacua aaaauggaua aauuaagacu caaaggaguu ucauacucau     1560

uauguacagc agccuuuacu uucaccaaaa uaccagcaga aacacugcau ggaacuguaa     1620

caguugaagu acaauacgca ggaacagaug gaccauguaa aguaccagcg cagauggcag     1680

uagauaugca aacacucaca ccuguaggua gacuaaucac agcaaauccg guaauaacag     1740

aaaguacaga aaauucuaaa augaugcuag aacuagaucc gccauucgga gacagcuaca     1800

uaguaauagg aguuggugag aagaaaauaa cacaccauug gcacagauca ggaaguacaa     1860

uaggaaaagc auuugaagca accguaagag gagccaaaag gauggcaguc uuaggagaua     1920

cagcauggga uuuuggaagu guagggggug ccuuaaauuc cuuggguaaa gguauacauc     1980

aaauuuuugg agcagcauuu aaaucauuau uuggcggcau guccugguuc ucacaaauac     2040

ugauaggaac ucuauuaaug uggcuuggcu uaaauacgaa aaauggaagu auaucauuga     2100

ugugccuagc auuaggcgga guucuaauau uuuuaaguac agcaguauca gcaugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6968
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6968
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

gaaaagaaaa gaaaagaaga ggagcggaca caaguguggg aauaguagga cuauuauuaa      120

caacagcuau ggcagcagag guaacgagga gaggaucagc auauuauaug uaucuagacc      180

gaaaugaugc aggagaagcu auuucauucc caacgacucu aggaaugaac aaaugcuaca      240

uacaaauaau ggacuuagga cauaugugug acgccacaau gaguuaugaa ugcccaaugc      300

uagaugaagg cguagaacca gaugacguag auuguuggug caauacaacc ucgaccuggg      360

uagucuacgg aacaugucac cauaagaaag gagaagcaag aaggucaaga cgagccguca      420

cuuuaccuag ccauucaaca cgaaaacuac aaacaagauc acaaacaugg cuagaaagua      480

gagaauauac caaacauuua auaagaguag aaaauuggau auucagaaac ccaggauucg      540

cauuagcugc agcagcuaua gcuuggcuau uaggcagcuc aacaagucaa aaagugauau      600

aucugguaau gauacuacuu auagcuccug cauacucaau uagauguaua ggcguaucaa      660

auagagauuu uguugaagga augagcggag gaacaugggu agacauagua uuagaacacg      720

guggaugugu gacaguuaug gcacaagaua aaccaacagu agauauugag cuaguuacua      780

caacaguauc aaacauggcc gaaguaagaa guuacugcua cgaagcauca auaagugaua      840

uggcaucaga uagcagaugu ccaacccaag gugaagcaua uuuagauaaa caaucagaua      900

cucaauaugu uuguaaaaga accuuaguag auagaggaug gggaaaugga uguggguuau      960

uuggaaaagg aaguuuagua acaugcgcua aauuugccug uucgaagaaa augacaggaa     1020

aaucuauuca accagaaaau cuagaauacc gaauaaugcu uagcgugcau ggaagucaac     1080

acagcggaau gauagucaac gauacuggac augaaacgga ugaaaacaga gcaaaaguug     1140

aaauaacacc gaauaguccc agagcagagg caacauuagg aggauuuggu uccuuaggac     1200

uagacugcga gccaagaaca ggccuagauu ucagcgauuu auauuaucua acuaugaaca     1260

auaaacacug guugguccau aaagaauggu uucaugauau acccuuaccu uggcaugcug     1320

gugcagauac aggaacacca cauuggaaua auaaagaagc acuuguagaa uuuaaagaug     1380

cacaugcaaa aagacaaaca guaguagucu uaggaucuca agagggagca guacacacgg     1440

cacuugcugg ggcauuagaa gcagaaaugg auggagcuaa aggucgauua aguaguggac     1500

acuuaaaaug ccgauuaaaa auggacaaau uaagauuaaa aggaguauca uauucacuau     1560

gcacagcugc uuucacauuu acaaaaauac cagcugaaac acuacaugga acaguaacag     1620

uagaaguaca auacgcagga acagauggac cguguaaagu accagcacaa auggcagugg     1680

auaugcaaac acuuacgcca guaggaagau uaauaacagc uaaucccgua auuacugaaa     1740

guacagaaaa uucuaaaaug augcuagaac uagacccacc uuucggugau aguuauauag     1800

uaauaggagu aggagaaaag aaaaucacac aucauuggca ucgaucagga aguacaauag     1860

gaaaagcauu ugaagcuaca guaagaggcg cuaaacgaau ggcaguucug ggagauacug     1920

caugggauuu uggaagugua ggaggagcac uaaacucauu aggaaaaggc auccaccaaa     1980

uauuuggagc ugcauuuaaa agucuauucg gagguauguc augguucagu caaauauuaa     2040

uaggaacguu auugaugugg uuaggcuuaa auacuaaaaa cgguucaauu ucacuaaugu     2100

gucuagcacu aggaggagua uuaauauucu uaaguacggc aguaagugca ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  6969
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6969
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

aagaaaaaaa aagaagaggu gcugauacaa guguaggaau cguaggacua uuauuaacaa      120

cagccauggc agccgaagua acaaggagag gcuccgcaua uuauauguau cuagaucgaa      180

acgaugcugg ugaagcaaua ucuuuuccaa caacacuagg aaugaauaaa uguuauauac      240

aaauuaugga ucuagggcau augugcgaug caacuaugag uuaugaaugc ccaauguuag      300

acgagggugu agaacccgau gauguugauu guugguguaa uacaacaagc acauggguag      360

uauauggaac augccaccac aaaaaaggug aggcaagaag aagccgaaga gcuguaacau      420

uaccaucaca uagcacuaga aaauuacaaa cgagaagcca aacaugguua gaaucaagag      480

aguacacuaa gcaucuaauu agaguagaaa acuggauauu uagaaaucca ggauucgcac      540

uugcagcagc ggcaauagca uggcuauuag gaucaucaac aagucaaaaa guaauauauu      600

uaguuaugau acuuuuaaua gcacccgcau auagcaucag augcauugga guaucaaaua      660

gagacuuugu cgaagguaug ucuggaggua ccuggguaga uauaguauua gaacauggug      720

gauguguaac gguaauggca caagacaaac caacugugga uauagaacua guaacuacaa      780

caguaaguaa uauggcagag guaagaucuu acugcuauga agcaucaaua ucagacaugg      840

caagcgacuc aagaugcccg acacaaggag aagccuauuu agauaaacaa ucggacacgc      900

aauauguuug uaaacgcacu uuaguagaua gaggaugggg uaauggaugu gguuuauuug      960

gaaaaggaag cuuaguuaca ugugcuaaau ucgcauguuc aaaaaaaaug acaggaaaau     1020

caauacaacc cgaaaacuug gaauaucgaa uaauguuaag cguacauggu ucacaacacu     1080

cagguaugau ugucaaugac acaggucaug aaacagacga aaacagagca aaaguagaaa     1140

uaacgccaaa cucaccuaga gcugaagcaa cacuaggugg uuuuggaucu cugggacuag     1200

auugcgaacc aagaacagga cucgauuuca gugauuuaua uuacuuaacu augaauaaua     1260

aacacuggcu aguccacaaa gaaugguuuc augauauacc acugccaugg cacgcaggug     1320

cagauaccgg uacaccccau uggaauaaca aagaagcacu aguggaauuu aaagaugcac     1380

augccaaacg ucaaacagug guaguuuuag gaagucaaga aggagcaguc cauacggcuc     1440

uagcaggugc acuagaggca gaaauggaug gggcuaaagg aagauuauca aguggacauu     1500

uaaaguguag acuaaaaaug gauaaauuga gauuaaaagg gguaaguuac ucauuaugca     1560

cagcugcauu uacauucaca aaaauuccag cagaaacacu acauggcaca guaacaguag     1620

aaguccaaua cgcaggaacc gaugguccgu guaagguacc ugcacaaaug gcaguagaca     1680

ugcaaacauu aacaccaguc ggccgacuaa uaaccgcaaa uccaguaauu acagaaagua     1740

cagaaaauag uaaaaugaug uuagaauuag auccaccauu uggagauucc uacauuguua     1800

uaggaguagg ugagaaaaaa auaacgcauc auuggcacag aaguggaagu acuauaggca     1860

aagccuuuga agcuacagua agaggggcga aaagaauggc aguauuagga gauacagcuu     1920

gggauuuugg aagugucggc ggcgcacuua auaguuuagg caaagguauu caccaaauau     1980

uuggagcggc auucaaauca cuauucggag gaaugucuug guuuucucaa auauuaauag     2040

gaacauuacu aaugugguua ggacuaaaua ccaaaaaugg cuccauauca cucauguguu     2100

uagcauuagg agggguacua auauucuuaa gcacagcagu aucagcauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  6970
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6970
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaag       60

aaaaaaaaag aagaggagca gauacaagug uagguauagu aggauuguua cuaacaacag      120

cuauggcagc agaaguaaca agaagaggaa gugcauauua uauguaccua gauagaaaug      180

augcagguga agcaauaagu uuuccaacaa cauugggaau gaauaaaugu uauauacaaa      240

uaauggauuu aggacauaug ugugacgcua ccaugucaua cgaaugucca augcuagaug      300

aaggaguaga accagaugau guagacuguu gguguaacac uacuucaaca uggguaguau      360

auggaacaug ccaucauaaa aaaggagaag caagaagauc aagaagagca guaacacuac      420

caucacauuc uacaagaaaa uuacaaacac gaagccaaac auggcuugaa ucacgagaau      480

auacaaaaca ucuaauacgu guagaaaacu ggauauuuag aaacccggga uucgcuuuag      540

cagccgcugc aauagcaugg cugcuagguu caucaaccuc acaaaaaguc auauaucuag      600

uuaugauacu acuaauagcu cccgcauacu caaucagaug uauaggagua agcaauagag      660

auuuuguuga aggaauguca ggagguacau ggguagauau aguguuagaa cauggaggau      720

guguaacagu aauggcgcaa gauaaaccaa caguagauau agaauuagua acaacuacag      780

uaaguaacau ggcugagguu agaaguuacu guuacgaggc gucuauaagc gauauggcau      840

cagacaguag auguccaacc caaggugaag cauauuuaga uaagcagagu gacacacaau      900

auguaugcaa aagaacguua guugaucgag gauggggaaa uggaugugga uuauuuggua      960

aaggaagucu aguaacaugu gccaaauuug cauguaguaa aaaaaugaca ggaaaaagca     1020

uacaaccuga aaaucuagaa uauaggauaa uguuaagugu acauggaagc caacacucug     1080

gcaugauugu aaaugacaca ggucaugaaa cggacgagaa cagagcgaaa guagaaauaa     1140

ccccgaauag ccccagagcu gaagcaacau uagggggauu ugguagucua gguuuagauu     1200

gugaaccaag aacaggguua gauuuuagug auuuauauua cuugacaaug aacaacaaac     1260

auuggcuagu acacaaagaa ugguuucacg auauaccacu acccuggcau gcuggagcgg     1320

acacagguac accccacugg aauaauaaag aagcucuagu cgaauuuaaa gaugcacaug     1380

caaaaagaca aacaguagua guuuuaggau cacaagaagg agcaguacau acggcacuag     1440

caggagcguu agaagcagaa auggaugggg cuaaagggcg auuaucauca ggacacuuaa     1500

aaugucgauu aaaaauggau aaacuaagau uaaaaggagu aucauauagu uuaugcacag     1560

cagcauuuac uuuuacaaaa auaccagccg aaacacuuca ugguacugua acaguggaag     1620

uccaguaugc uggaaccgau ggaccaugua aagugccagc gcaaauggca guagauaugc     1680

aaacauuaac cccgguaggc agacuuauaa cugccaaccc uguaauaaca gaaucaacgg     1740

aaaacucaaa aaugaugcuu gaauuagauc cgccauuugg agacagcuac auuguaauag     1800

guguaggaga aaaaaaaaua acacaccacu ggcauagguc aggaucgacc auugguaaag     1860

cguuugaagc aacaguacga ggagcuaaaa gaauggcagu acuuggagac acagcuuggg     1920

auuuugguuc uguaggaggu gcauuaaaca gccuaggaaa aggaauacau caaauauuug     1980

gagcagcuuu uaaaagucua uuugguggaa ugaguugguu uagccaaaua uuaauuggaa     2040

cguuauuaau gugguuaggg uuaaauacaa agaacgguag uauuagucuu augugcuuag     2100

cuuuaggagg aguacuaaua uuuuuaagca cagccguauc agcaugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  6971
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6971
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaaa       60

agaaaagaag aggggcggau acauccguag gaauaguugg acuauuauua acaacugcaa      120

uggccgcaga aguaacacgu agaggaucag cuuauuauau guaucuagau agaaacgaug      180

caggagaagc uauaaguuuu ccaacaacac uaggaaugaa uaaauguuau auacaaauaa      240

uggaccucgg ccacaugugu gacgcuacaa ugucuuauga augucccaug uuagaugaag      300

gagucgaacc agaugacgua gauugcuggu guaauacaac aucuacuugg guaguauaug      360

guacauguca ucacaagaaa ggugaagcaa gaagaucacg aagggcagua acuuuaccau      420

cucacagcac uagaaaauua caaacuagaa gccaaacaug guuagaaucu agagaauaua      480

caaaacauuu aauacgagua gaaaauugga uauuuagaaa cccugguuuu gccuuggcag      540

ccgccgcuau agcaugguua cuaggaucau caacaagcca aaaaguaaua uaccuaguua      600

ugauacuacu aauugcacca gcauauucaa uuagguguau aggaguuagc aauagagauu      660

uuguagaagg uaugucaggu ggaacauggg uagacauugu auuagaacac ggagguugug      720

ugacuguaau ggcgcaagau aaaccaacag uagacauaga auuaguaacu acaacuguaa      780

guaacauggc agagguaaga ucguauugcu augaagcaag uauaagugac auggcuucag      840

auucaagaug uccaacacaa ggugaggcau aucuagacaa acaaucagac acacaauacg      900

uauguaaaag aacuuuagua gaccgcggcu ggggaaacgg augcggacua uuugguaaag      960

gaaguuuagu aacaugcgca aaauucgcau guucuaagaa aaugacagga aaaucaauac     1020

aaccugaaaa ucuagaauau agaauaaugc uaaguguaca uggaagucaa cauucaggaa     1080

ugauugugaa cgacacugga caugaaaccg augaaaauag agcaaaagua gaaauaacac     1140

caaauucgcc aagagcagaa gcaacucuug gggguuuugg aucauuggga uuagauugcg     1200

agccuagaac aggauuagau uucagcgauc uuuauuacuu gacaaugaau aauaaacauu     1260

gguuaguaca uaaagaaugg uuccaugaua uaccacuacc auggcaugcg ggagcagaua     1320

cugguacacc ucauuggaac aauaaagaag cacugguaga guucaaagau gcccaugcaa     1380

aacgacaaac aguaguagua uuagggucac aagaaggagc cguucacaca gcacucgcug     1440

gcgcauuaga agccgaaaug gauggcgcaa aaggaagauu gucuucagga caucuaaaau     1500

gccgauuaaa aauggacaaa uuaagacuaa aagggguaag cuauucauua uguacagcgg     1560

cauuuacauu uacaaaaaua ccugcagaaa cucuacaugg aacaguaaca guggaaguac     1620

aauaugcagg uacugacggu ccuuguaaag uaccagcaca aauggcagua gacaugcaaa     1680

cuuuaacacc aguagggaga uuaauuacug cgaauccagu uauaacagaa aguacagaaa     1740

auucaaaaau gauguuagaa uuagauccac cauucggaga uagcuauaua guaauaggug     1800

uaggagaaaa gaaaauaacu caucacuggc acagaagugg auccacaaua ggaaaagcau     1860

ucgaagcaac aguaagaggc gcaaaaagaa uggcaguauu aggcgacacu gcaugggauu     1920

uugggucagu aggaggagca uugaauaguc ucgguaaagg uauccaucaa auauuuggag     1980

ccgccuuuaa aaguuuauuc ggaggaaugu cuugguuuuc ucaaaucuua auagguacac     2040

uacuaaugug gcucggacua aauacgaaaa auggcucgau aucacuaaug uguuuagcuc     2100

uaggaggugu acuaauauuu uuaucaacag cagugagcgc cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  6972
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6972
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aaagaagggg agcagauaca aguguaggaa uaguaggacu auuacugaca acagcaaugg      120

ccgcggaagu aacaagaaga ggaucagcuu acuacaugua cuuagaccga aaugaugcag      180

gagaagcaau aucguuucca accacauuag gaaugaauaa auguuauaua caaauaaugg      240

auuuaggaca uaugugugac gcaacaauga gcuaugaaug cccaaugcua gaugaaggag      300

ucgaaccuga ugauguagau uguuggugca acacaacuag cacuugggua guauacggaa      360

caugucacca uaagaaaggu gaagcuagac gaaguaggag agcuguaaca uuacccaguc      420

auagcacaag aaaauuacag acaagaagcc aaacaugguu agaaucaaga gaauacacaa      480

aacaccuaau aagaguagaa aauuggauau uuagaaaucc aggcuucgcu cuggcagcag      540

cagcgauagc cuggcuacua ggaaguucga caagucaaaa aguaauauau uuaguuauga      600

uuuuacugau ugcuccagca uauaguauaa gauguauagg agucaguaac cgagauuuug      660

uagaaggcau gucaggagga acauggguag auauuguauu agaacaugga gguuguguca      720

caguaauggc acaagacaaa ccaacaguag auauagaauu aguaacaacu acaguaagua      780

auauggcaga gguaagauca uauuguuaug aagcauccau aagugauaug gcaucagauu      840

caagaugucc uacucaggga gaagcauauu uagauaaaca aagugauaca caauauguau      900

guaaaaggac auuaguagac cguggauggg gaaauggaug uggacuauuu ggaaaaggau      960

cauuaguuac augcgcaaaa uuugcauguu cuaagaaaau gacagguaaa agcauacaac     1020

cagaaaauuu agaauaucgc auuaugcuau caguucaugg aucccaacau ucagguauga     1080

ucgugaacga uacagggcau gaaacagaug aaaauagagc aaaaguagaa auaacaccaa     1140

acagcccaag agcagaagca acuuuaggcg gcuuuggaag uuuaggauua gacugugaac     1200

caagaacagg uuuagauuuu ucagaucuau acuauuuaac aaugaacaau aaacauuggu     1260

uaguccauaa agaaugguuu cacgacauac ccuuaccaug gcacgcgggu gcagauacag     1320

guacuccaca uuggaacaac aaagaagcau uaguagaauu uaaggacgcu caugcaaaaa     1380

ggcaaacugu gguaguauua ggaucacaag agggagcugu ucacacagca uuagcaggag     1440

cucuagaagc agagauggau ggagcaaaag gaagauuaag uucgggacau cuaaaauguc     1500

gacuaaaaau ggacaagcua agauuaaaag gaguaaguua uagucuaugc acagcagcuu     1560

uuacauuuac caaaauacca gcugaaacgc uacaugggac aguaacagua gaaguacaau     1620

augcagguac agaugggccu uguaaaguac cagcacaaau ggcaguagau augcaaacac     1680

uaacaccagu aggacgauua auaacagcaa auccagugau uacugaaagc acagaaaaca     1740

guaaaaugau gcuagaauua gacccacccu uuggagacag uuauauagua auaggcguag     1800

gagaaaagaa aauaacucau cacuggcaua gaaguggaag cacaauagga aaagcauuug     1860

aagcaacagu uagaggagca aaacgaaugg cugugcuagg agauacagcu ugggauuuug     1920

gcucaguagg aggggcauua aauucauuag guaaaggaau acaucaaaua uucggugcag     1980

cauuuaaaag ucuauuuggu ggaaugaguu gguuuucaca aauauuaaua gguacucuac     2040

uaaugugguu gggauuaaac acgaaaaacg gaucaaucag ucuaaugugc cuagcgcuug     2100

ggggcguguu aauauuuuua aguacagcag uaagugcaug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  6973
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6973
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

gacguggagc ugauacaagu gucggcauug uaggucuguu auuaacaaca gcaauggccg      120

cagaaguaac aagaagagga ucagcauauu auauguauuu agauagaaau gaugccggag      180

aagcaauauc uuuuccuaca acauuaggga ugaauaaaug cuauauucaa auaauggauu      240

uagggcacau gugugaugca acgaugaguu acgaaugccc aauguuagau gaaggaguag      300

aaccggacga uguagauugu ugguguaaca cuacaucaac auggguuguu uacggaacau      360

gucaucauaa gaaaggagaa gcaaggagaa gucgcagagc agucacauua ccuagucacu      420

caacaagaaa auuacaaaca agaagucaaa caugguuaga aaguagagaa uauaccaaac      480

aucuaauaag agucgaaaau uggauuuuua gaaacccggg auucgcacug gcagcagcug      540

ccauugcaug gcuccuggga ucaaguacgu cacaaaaagu aauauauuua guaaugaucu      600

uauuaaucgc accggcauac ucaauuagau guauaggagu aagcaacaga gauuuuguag      660

aagggauguc agguggaaca uggguagaua ucguauuaga acauggcggg ugugucacag      720

uaauggcaca agauaaaccu acaguugaua uagaauuagu gacaaccaca guuaguaaua      780

uggcagaagu acgaucauau uguuaugagg cuuccauauc agacauggca agugacucac      840

gaugcccaac ucaaggagaa gccuauuuag acaaacaauc ugauacacaa uacguaugca      900

aaaggaccuu agucgacaga gguuggggaa auggaugcgg acuauuuggg aaagguagcu      960

uaguaacaug ugcaaaauuu gcaugcucga agaaaaugac aggaaaaagc auacaaccag     1020

aaaauuuaga auauagaaua auguuaagcg uucauggauc acaacacagu ggaaugauag     1080

uaaaugauac agggcaugaa acagacgaaa auagagcuaa aguagaaaua acuccaaaca     1140

gcccaagagc ggaagcaaca uuaggaggau uuggaaguuu aggacuagau ugcgaaccca     1200

gaacaggacu ggacuuuagc gaccuauauu auuuaacaau gaauaauaaa cauuggcugg     1260

uucauaaaga augguuucau gauauuccuu uaccguggca ugccggugca gauacaggua     1320

caccacauug gaauaacaaa gaagcacucg uagaauuuaa agaugcacau gcaaaaagac     1380

aaacaguagu agucuuagga ucccaagaag gagcaguaca cacugcucua gcgggagccc     1440

uagaagcaga aauggacggc gcuaaaggaa gauuaucuuc aggacaucua aaauguagac     1500

uaaaaaugga uaagcuaaga uuaaaaggcg uaucuuauag ccuauguacu gcagcuuuua     1560

cauuuacaaa aauaccagca gaaacuuuac auggaacagu aacaguagaa guacaauacg     1620

caggaacuga cggaccaugc aaaguucccg cacaaauggc agucgacaug cagacacuaa     1680

caccaguugg aagauuaaua acagcaaauc caguaauaac agaaucaaca gaaaauucua     1740

aaaugaugcu ugaauuagac ccgcccuucg gagauucaua uauaguaauu gguguaggag     1800

aaaagaaaau aacccaucac uggcacagaa guggauccac aauagguaaa gcauuugaag     1860

caacaguaag aggugccaaa aggauggcag uccuaggaga uacagccugg gauuuuggaa     1920

guguuggugg ggcacuuaac aguuuaggaa aaggaauaca ucaaauuuuu ggagcagcau     1980

ucaaaagucu auucggagga augaguuggu uuucacaaau acuaaucgga acacuauuaa     2040

ugugguuagg auuaaauacu aaaaacggaa gcauaucauu aaugugucuu gcauuaggcg     2100

gagugcuaau cuuuuuaagc acagcaguaa gcgcaugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  6974
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6974
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ggggugcgga uacaucugua ggaauaguag gauuauuauu aacaacggca auggcagcag      120

aaguuacaag aagaggaagu gcuuacuaca uguauuuaga uagaaacgau gcuggugaag      180

ccauaaguuu cccaacuaca uuaggaauga acaaaugcua cauccaaauc auggaccuug      240

gacauaugug ugaugcuaca augucauacg aauguccaau gcuggaugaa gguguagaac      300

ccgaugaugu agacuguugg uguaacacaa caucuacuug gguuguuuau gguacguguc      360

accauaagaa aggugaagca agacgaucaa gacgagcagu aacauuaccc agucacucaa      420

ccagaaaacu acaaacaaga ucacaaacau gguuagaauc aagagaauau acaaaacauc      480

uuauaagagu agaaaacugg aucuuuagaa acccuggauu ugcauuagca gcagcagcua      540

uagcaugguu acuagguucg aguacaaguc aaaaaguuau uuaucucgua augauacuau      600

uaauagcacc agcuuauagc auaagaugua uagguguauc aaauagagau uuuguagaag      660

ggaugucagg agggacaugg guagauaucg uauuagaaca uggaggaugu guaacaguca      720

uggcgcaaga caaaccaaca guugauauag aauuaguaac aacaacaguu uccaauaugg      780

cagaaguuag aucauauugu uaugaagcua gcaucucaga uauggcuucc gacucaagau      840

guccaacaca aggagaagca uauuuagaca aacaaucuga uacacaauau guauguaaaa      900

gaacucuagu agauagaggu uggggaaaug guuguggauu auuuggcaaa ggaucuuuag      960

uuacaugcgc aaaauuugca uguaguaaga aaaugacagg aaaaagcauu caaccugaaa     1020

auuuagaaua uagaauaaug cuaucaguac acggaaguca acauagcggu augauaguaa     1080

acgauacagg acaugaaaca gaugaaaaca gagcaaaagu ggaaauaaca ccaaacucac     1140

cuagagcaga agcaacauua ggcggauuug guaguuuagg auuagacugu gaaccaagaa     1200

caggguuaga uuuuagugau uuguauuauu uaacaaugaa uaacaaacau uggcuaguac     1260

auaaagaaug guuccacgau auaccauuac cguggcaugc aggggcagau acuggaacuc     1320

cccauuggaa caauaaagaa gccuugguag aauuuaaaga cgcacaugca aaacgacaaa     1380

cagucguagu auuaggcuca caagaaggcg caguacauac cgcuuuagca ggcgcuuuag     1440

aagcagaaau ggauggagcc aaaggaaggc uaucaucugg ucaucuuaaa uguagacuaa     1500

aaauggauaa acuaagacua aaaggaguuu cuuacagucu cuguacagca gcauuuacau     1560

ucacgaaaau accagcugaa acacuacaug gaacuguaac aguagaagua caauaugcag     1620

gaaccgaugg accauguaaa guaccagcac aaauggcagu ugauaugcaa acauuaaccc     1680

caguagggag acuaauaacc gcaaauccag uuauuacaga aucaacagaa aacaguaaaa     1740

ugaugcuaga auuagauccc ccuuuuggcg auucauacau cguaauaggu gugggagaaa     1800

agaaaauaac gcaucacugg caucgaucag gaagcacaau aggaaaagca uuugaagcua     1860

cagucagagg agcuaaaaga auggccguuc uaggagacac ugcuugggac uucggaucag     1920

uugguggagc auuaaauucu cugggaaaag gaauacauca aauauuuggu gcagccuuca     1980

aaucacuauu ugguggcaug aguugguucu cacagauacu aauagguacc uuauuaaugu     2040

ggcuaggauu aaauacuaaa aacggaucaa uaucauugau gugcuuagcc cuaggaggag     2100

uuuuaauauu ucuaagcaca gcugucagug caugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  6975
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6975
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gagccgauac aagcguugga auaguugguu uauuauuaac aacagcuaug gcagcagaag      120

uaaccagacg uggaucggca uauuauaugu accuagacag gaaugaugca ggagaagcaa      180

uaucuuuucc cacaacuuua ggaaugaaua aaugcuauau acaaauaaug gauuuaggac      240

auauguguga ugcaacuaug agcuaugaau gcccuauguu ggaugaaggg guugaaccag      300

augauguaga uuguuggugc aacacuacau caaccugggu uguauaugga acaugucauc      360

acaagaaagg agaagcucgc agaagcagac gagcaguaac acuaccuuca cauagcacaa      420

gaaaacuaca aacacgaucu caaacauggu uagaaagcag agaauacaca aaacaccuaa      480

uaaggguaga aaauuggaua uuuagaaacc caggguuugc auuagcagca gcggcuauag      540

ccuggcuauu aggaucuagu acaucacaaa aaguaaucua cuuagugaug auauuauuaa      600

uugcaccagc auacucaaua agauguauag gaguuagcaa uagagauuuc gucgaaggaa      660

ugucaggcgg aacaugggua gauauuguau uggaacaugg aggaugcgua acaguaaugg      720

cacaagauaa accuacugua gacauagaau uaguaacaac aacuguaagc aacauggcag      780

aaguaagguc auauuguuac gaagcaagua uaucagauau ggcaagugau ucgagauguc      840

caacacaagg cgaagcauau uuagacaaac aaucagauac acaauaugua uguaaaagaa      900

cccuaguaga uagaggaugg ggaaauggau guggucuauu uggaaaagga aguuuaguaa      960

caugcgcuaa auucgcaugc agcaagaaaa ugacaggaaa aucuauacaa ccagaaaauu     1020

uagaauacag gauaauguua ucaguccacg gaagucaaca uucaggaaug auagucaaug     1080

acacaggaca ugaaacugac gaaaauagag caaaaguaga aauaacacca aacucaccaa     1140

gagcagaagc aacacuaggu ggcuucgguu cauuaggacu ggacugugaa ccaagaacag     1200

gauuagacuu cagcgauuua uacuacuuaa cuaugaauaa caaacauugg cuaguacaua     1260

aagaaugguu ccaugacaua ccauuaccuu ggcaugcugg ugcagauacu ggaacaccac     1320

auuggaauaa uaaagaagcg cuaguggagu ucaaagaugc acaugcuaaa cgacaaacag     1380

uaguaguauu aggcucacaa gaaggagccg uccauacagc acuugcagga gcacuugaag     1440

cagaaaugga uggagcaaaa ggaagauuau cgaguggcca cuuaaaaugu cgacuaaaaa     1500

uggauaaacu aagauuaaaa ggaguuucau auaguuuaug uacagcagca uuuacauuca     1560

ccaaaauucc agcagaaaca uuacauggaa ccguaacugu ggaaguacaa uaugcaggaa     1620

cagauggacc cuguaaagua ccagcacaaa uggcagucga uaugcaaacc uuaacaccag     1680

uaggaagauu aaucaccgca aacccuguga uaacagaauc uacagaaaac aguaaaauga     1740

ugcuagaauu ggauccucca uucggagauu ccuauauagu aauaggagua ggagaaaaga     1800

aaauuacuca ccacuggcac agaucaggua gcaccauagg uaaagcauuu gaagcuacag     1860

uaagaggugc aaaaaggaug gcuguauuag gcgacacagc augggauuuc ggaucaguug     1920

gaggcgcuuu aaacucacuc ggaaaaggca uucaucaaau auuuggagca gcauuuaaaa     1980

guuuauuugg aggaauguca ugguuuucac aaauucuaau aggaacauua cuaauguggc     2040

ugggucucaa uaccaaaaac ggcaguauca gcuugaugug ucuagcauua ggaggcguac     2100

uaauauuccu aagcacagca guaucagcuu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6976
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6976
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

cagauacaag uguaggaauu guaggguuau uacuaacaac agcaauggca gcagaaguaa      120

caagaagagg uagugcauau uauauguauc uugauagaaa cgaugcagga gaagcuauuu      180

cauuuccaac aacacuaggc augaauaaau guuauauaca aaucauggau uuaggacaca      240

ugugcgaugc uacaaugagu uaugaauguc caaugcuaga cgaaggcguu gaaccagaug      300

auguagauug cugguguaac acaacaagua cuuggguagu uuauggcacc ugucaucaca      360

agaaaggaga agcgcgaaga aguagacgag caguaacauu accaucacau agcacaagaa      420

aauugcagac uagaagucaa acauggcuug aaucaagaga auauacaaaa cauuuaauac      480

gaguugaaaa cuggauauuc cgaaauccag guuuugcacu agcagcagca gcaauagcau      540

ggcuacuggg aucaaguacu agccaaaaag uaauauauuu aguaaugaua uuacuaauag      600

cccccgcgua caguauaagg ugcauaggug uaucgaauag ggauuuuguu gaaggaauga      660

gcggagggac uuggguggau auaguacuag aacauggagg uuguguuaca guaauggcac      720

aagauaaacc aacaguugac auagaauuag ugacaacaac uguaagcaau auggcugaag      780

uaaggagcua uugcuaugaa gcaaguauau cagauauggc aagugauuca agauguccaa      840

cacaagguga agcauacuua gauaaacaau ccgauacuca auauguaugu aaaagaacuc      900

uaguagaucg aggaugggga aacggaugug gauuauuugg aaaagggagu uuaguaacau      960

gcgcaaaauu ugcauguagu aagaaaauga caggaaaauc aauacagcca gaaaaucuag     1020

aauaucggau aaugcuauca guacauggua gucaacauuc aggaaugaua guaaacgaua     1080

caggucacga aacagacgaa aaucgagcaa aaguugaaau aacaccuaac aguccacgag     1140

cugaagcaac auuaggcgga uuuggauccc uaggauuaga cugugaaccg aggacaggac     1200

uagauuuuuc agaucuauau uauuuaacaa ugaacaauaa acauuggcua guucauaaag     1260

aaugguuuca ugauauacca uuaccauggc augcuggugc agacacagga acaccucauu     1320

ggaauaauaa agaagcauua guagaauuua aagacgcaca cgcaaaaaga caaacaguug     1380

uaguauuagg aagccaagaa ggagcuguac auacagcacu ugcuggagca cuagaagcug     1440

aaauggaugg ugcuaaaggc agauuaagca guggucauuu aaaaugcaga uugaaaaugg     1500

auaaauuacg auuaaaagga guaucauauu ccuuaugcac agcagcauuu acauuuacaa     1560

aaauaccagc agaaacauua cauggaacag uaacaguaga aguccaauau gcaggaacag     1620

acggaccaug caaaguacca gcacaaaugg caguugauau gcagacacua acaccaguag     1680

gaagauuaau aacugcaaac ccgguaauaa ccgaaaguac agaaaauuca aaaaugaugu     1740

uggagcugga uccaccauuu ggggacucau acauaguaau uggcguagga gaaaagaaaa     1800

uuacacacca cuggcaucga aguggaagca caauaggaaa agcauucgaa gccacaguaa     1860

ggggagcaaa aagaauggca guauuaggug acacagcaug ggacuucggu ucaguaggag     1920

gugcuuuaaa uaguuuaggu aaaggaauac accaaauauu uggagcagca uuuaaaucau     1980

uauuuggagg aaugaguugg uuuucacaaa uucugauagg aacguuacua auguggcuug     2040

gcuuaaauac gaaaaaugga agcauuucau uaauguguuu agcgcuuggu ggaguuuuaa     2100

uauuuuuauc uacugcaguu agcgcgugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  6977
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6977
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcag       60

auacgucugu cggaauaguu ggauuacuac uaacaacugc aauggccgca gaagugacua      120

gacgagguuc ggcguacuac auguaucuag acagaaauga ugcuggagaa gccauaucau      180

uuccaacuac auuaggaaug aauaaaugcu acauacaaau uauggaccua ggacauaugu      240

gugacgcaac aaugucauau gaauguccaa uguuagauga agggguagaa ccagacgacg      300

uagauugcug guguaacaca acaucuacau gggugguuua cgguacaugu caucauaaga      360

aaggugaagc aagacgcuca agacgagcag uaacacugcc aucacauagu acaagaaaac      420

uacaaacaag aucacaaaca uggcucgaau cgagagaaua cacaaaacau cuuauaagag      480

uagaaaauug gauauucaga aaucccggcu ucgcacuagc ugcagcagca auagcauggu      540

uacuuggaag uucuacgagu caaaaaguaa uauaucuagu aaugauacuu cuaaucgcgc      600

cugcauauag cauaaggugu aucggggucu caaauagaga cuuuguagaa ggaaugucag      660

gaggaacaug gguagauauu guguuagaac augguggaug ugucacagua auggcacaag      720

acaaacccac aguagacaua gaauuaguua cuaccacagu aucaaacaug gcagaaguuc      780

gaucguauug uuaugaagca uccauaagug auauggcauc agacaguaga ugcccuaccc      840

aaggagaagc auauuuagau aaacaaagcg auacucaaua cguauguaaa agaacccuag      900

uagaucgugg augggguaac ggaugugguu uauuugguaa aggcaguuua guaacuugug      960

caaaguuugc auguucaaag aaaaugacag guaaaucuau acaaccggaa aaccuagaau     1020

aucgaauaau gcuaagugua cauggaucuc agcauagugg aaugauagua aaugacacag     1080

ggcaugaaac agaugaaaac agagcgaaag uagaaauaac uccaaacagc ccaagagcag     1140

aagcaacauu aggaggauuu ggcagucuag gacuagauug ugaaccaaga acuggauuag     1200

auuuuucaga ucuuuacuac uuaacuauga acaacaaaca uugguuagua cacaaagaau     1260

gguuucauga cauaccgcua ccauggcacg cuggugcaga uaccggcaca ccucauugga     1320

auaauaaaga agccuuagua gaauuuaaag acgcacaugc aaaaagacaa acaguaguag     1380

uacuaggauc acaggaagga gcaguacaua cagcccuagc uggagcauua gaagcggaaa     1440

uggacggagc uaaaggaaga uugucaagcg gacacuuaaa augucgacua aaaauggaua     1500

aacuaagacu uaaaggagua ucauauagcu uaugcacagc agcauuuaca uuuacaaaaa     1560

uaccggcaga aaccuugcau gggacuguaa caguagaagu acaauaugca gggacagaug     1620

gcccauguaa aguaccagca caaauggcag uagauaugca aacccuaacu ccaguaggaa     1680

gauuaauaac cgcaaaucca guaauaacug aaagcacgga aaacucaaaa augaugcuag     1740

aacuagaucc accauucgga gacaguuaua uaguaauagg cguaggagaa aagaaaauaa     1800

cgcaccauug gcauagaagc ggaaguacaa ucggaaaagc auuugaagca acaguaagag     1860

gcgcaaaaag aauggcagua cuaggagaua cagcauggga cuuuggaucg guaggagggg     1920

cauuaaacag uuuagggaaa ggaauacauc aaauauuugg agcagcauuu aaaucacuau     1980

ucggaggaau gagcugguuc ucacaaauau uaauagggac auuauugaug uggcuaggcc     2040

uuaacacuaa aaauggauca auaagucuaa uguguuuggc auuaggcgga guacuaauau     2100

uuuuaucaac agcaguauca gcaugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  6978
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6978
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaua       60

ccucaguugg aauaguagga cugcuauuaa caacagcuau ggcugccgaa guaacaagaa      120

gaggaagcgc auacuauaug uacuuagaua gaaaugaugc gggagaagca auaaguuuuc      180

caaccacacu aggaaugaau aaauguuaca uacaaauaau ggaucuaggg cauaugugug      240

augcgacaau gucauaugaa uguccaaugu uggaugaagg cguagaaccc gaugauguag      300

acuguuggug uaacacaaca agcacauggg uaguauaugg aacaugucau cacaagaaag      360

gagaagcacg aagaaguagg agagcaguua cauuaccauc acauaguacu agaaaauugc      420

aaacuagauc acaaacaugg uuagaaagcc gcgaauauac aaaacauuug auaagaguag      480

aaaauuggau uuuuagaaac ccaggcuucg cacuagccgc agcagcaauc gcauggcuuc      540

uaggaagcuc uaccucacaa aaaguaauau auuugguaau gauauuauua auagcaccag      600

cuuauaguau aagauguauc ggaguaagua auagagauuu uguagaagga augaguggag      660

gaacaugggu agacauaguc uuagaacaug gaggaugugu uacaguaaug gcgcaagaua      720

aaccaacagu agauauagaa uuaguaacca caacaguaag uaauauggca gaaguaagaa      780

guuauuguua ugaagcaagc auuagugaua uggcaucaga uagucgaugu ccaacacagg      840

gagaagcaua cuuggacaaa caaagugaca cucaguaugu auguaagaga acauugguag      900

auagaggaug ggguaauggu uguggauuau ucggaaaagg aucguugguc accugugcaa      960

aauuugcuug uagcaagaaa augaccggaa aaucaauuca gccagaaaac uuagaauaua     1020

gaauaauguu aagcguucau ggaagucaac auagcggcau gauaguaaau gauacaggac     1080

acgaaacaga cgaaaacaga gcaaaaguag aaauuacacc aaauagucca agagcagaag     1140

caacauuagg agguuuugga ucucuuggau uggauuguga accaaggacc ggacuggauu     1200

uuucagaccu auauuacuua acaaugaaua auaaacauug guuaguacac aaagaauggu     1260

uucacgacau uccauuaccu uggcacgccg gagcagauac ugggacaccg cauuggaaua     1320

auaaagaagc auuaguagaa uuuaaagacg cacacgcaaa acgacaaaca guaguuguuc     1380

uaggaaguca agagggggca guucauacag ccuuagcagg ggcacuagag gcagaaaugg     1440

auggagcuaa aggaagacua aguaguggac acuuaaaaug uagauuaaaa auggacaaau     1500

uacgucucaa gggagucucc uauagcuugu guacagcagc auuuacuuuc acaaaaauac     1560

cagcagaaac uuuacaugga acaguaaccg uagaaguaca auaugcagga acagauggac     1620

caugcaaagu accagcacaa auggcagucg acaugcaaac acuaacacca guagggagac     1680

uaaucacagc aaauccugua auaacagaau caacugaaaa uagcaagaug auguuagaau     1740

uagauccacc auuuggagau ucauacauag uaauuggagu aggugaaaag aagauaacac     1800

aucacuggca cagaagugga ucaaccauag gaaaggcuuu ugaagcaacc guaagaggug     1860

caaaacgaau ggcuguuuua ggagacacag caugggacuu ugggucagua gguggagcau     1920

uaaauagucu aggaaaaggu auucaucaaa uauuuggagc agcuuuuaaa aguuuauucg     1980

gaggaauguc augguucagc caaauauuga uaggaacuuu auuaaugugg cuaggauuga     2040

auaccaaaaa ugguaguauu agucuaaugu gucuugcguu aggaggagua uuaauauuuu     2100

uaagcacagc aguaucagca ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  6979
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  6979
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggaau aguuggauua uuacuaacaa cagcaauggc agcugaagua acuagaagag      120

gaagcgcaua uuauauguac cuagaucgaa acgacgcugg agaagcaaua ucuuucccaa      180

cuacauuagg caugaauaaa ugcuauauac aaauaaugga uuuaggacau augugcgaug      240

caacaauguc auacgaaugu cccauguuag acgaaggagu agaaccagau gauguagauu      300

guuggugcaa uacaacauca acaugggucg ucuauggcac augucaucau aagaaaggug      360

aagcacggag aagucgcaga gcaguuacac uaccgucaca uucaacaaga aaacuacaaa      420

cuagaucaca aaccugguug gaaaguagag aauauacaaa acaucuaaua aggguagaaa      480

auuggauauu cagaaacccc ggauuugcuu uagcugcagc agcaauagca uggcuacuag      540

gaaguagcac aucacaaaaa guaauauauc uaguaaugau acuacuaaua gcgccagcgu      600

auaguauaag auguauagga guaucuaacc gugauuuugu ggaagggaug ucagguggaa      660

cauggguaga uauuguauua gaacauggag gauguguaac agucauggcg caagacaaac      720

caacuguaga uauagagcua guaaccacaa cuguaucaaa uauggcagaa guuagauccu      780

auugcuacga agcaucgauu agcgauaugg caagugauuc cagaugucca acccaaggag      840

aggcuuacuu agauaaacaa agugauacac aauauguaug caaaagaaca uuagucgaua      900

gaggaugggg aaauggaugc ggguuauucg gaaaaggauc uuuagugaca ugugcaaaau      960

uugcuuguuc uaagaaaaug acaggcaaau caauacaacc agaaaacuua gaauauagaa     1020

uuauguuauc aguacacgga ucacaacaua gcggaaugau ugucaaugau acaggacaug     1080

aaacagauga aaauagagca aaaguagaaa uaacaccuaa uucaccuaga gcagaagcaa     1140

cacuaggagg auucggaagu uuagguuugg auugcgaacc acgaacuggu cuagauuuuu     1200

cagauuuaua cuauuugaca augaauaaua aacauugguu gguacauaaa gaaugguuuc     1260

augacauacc acuaccuugg caugcaggag cagacacugg aacaccacac uggaacaaua     1320

aagaagcacu ugucgaguuc aaagaugcac augcuaaaag acaaacaguu guuguauuag     1380

gaagccaaga aggagcagua cauacagcau uagcaggggc guuagaagca gaaauggaug     1440

gagcaaaagg cagacuuucu uccggacauu uaaaauguag auuaaaaaug gauaaauuac     1500

gccuaaaagg ugucucauac ucauuaugua cagcagcauu uaccuuuacc aaaauuccag     1560

cggaaacccu ucauggaacc guaacaguug aaguucaaua ugcuggcaca gacggaccau     1620

gcaaaguacc ugcacaaaug gcaguagaua ugcaaacucu aacuccaguu gggagacuaa     1680

uaacagccaa uccaguuauc acagaaagca cagaaaauuc aaagaugaug cucgaauuag     1740

auccgccauu cggugacucu uauauaguua uaggaguagg ugaaaagaaa auaacacauc     1800

auuggcaccg uagcggaagc acuauuggaa aagcauuuga agcaacaguu agaggugcaa     1860

aaagaauggc aguacuagga gauaccgcau gggauuucgg aagugucggc ggagcacuaa     1920

auucauuagg uaaagguaua caucaaauau uuggagcagc uuuuaaaagc cuauuuggug     1980

gaaugaguug guucucgcaa auauuaauag gaacccuuuu aauguggcua ggacuaaaua     2040

cuaaaaaugg aaguauaagc cucauguguc uagcacuugg gggaguauua auauucuuau     2100

ccacagcugu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6980
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6980
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacgu       60

caguaggaau ugucggauua uuauuaacua cagcaauggc agcagcugua accagaaggg      120

gaucagccua cuauauguau uuggaccgaa augaugcugg ugaagcaaua ucauuuccaa      180

cuacauuagg uaugaauaaa ugcuauauac aaauaaugga ucuuggacau augugugaug      240

caaccaugag uuaugaaugu ccuauguuag augaaggagu agaaccugau gacguagauu      300

gcugguguaa cacaacuuca acauggguag uauauggaac augccaucac aagaaaggag      360

aagcaagacg aagccggaga gcaguaacau uaccaaguca cucaacgaga aaauuacaaa      420

caagaucaca aacauggcuu gaaaguagag aauacacuaa acauuuaaua agagucgaaa      480

acuggauauu uagaaaucca ggauuugcac uagcagcugc agccauugca ugguuauuag      540

gaucaaguac aucucaaaaa gucauuuacu uaguaaugau auuacucaua gcuccugcau      600

auucaaucag augcauaggc guuucaaaca gagauuucgu agaaggaaug ucagguggaa      660

cauggguaga uaucguacua gaacacggag gaugcguuac aguaauggca caggauaaac      720

caaccguaga cauagaauua guaacaacua cuguaaguaa uauggccgaa guaagaucau      780

auuguuauga agccaguauu agcgauaugg ccagcgauag cagaugucca acgcaaggag      840

aagcauacuu agauaaacaa agugauacuc aauauguaug uaaacgaaca uuaguagauc      900

gaggaugggg uaacggaugc gguuuauuug gaaaaggauc auuaguaaca ugcgcaaaau      960

uugcaugcuc uaagaaaaug acuggaaaau caauacaacc agaaaaucua gaauacagaa     1020

uaauguuauc gguacaugga agccaacauu caggaaugau aguaaacgac acuggacaug     1080

aaaccgacga aaauagagcc aaaguagaaa uaacaccuaa uuccccaaga gcagaagcaa     1140

cauuaggagg auucggaagc cuaggacuag auugugaacc aagaacuggu cuugacuucu     1200

cagacuuaua cuaccuaacc augaacaaca aacacugguu aguacauaaa gaaugguucc     1260

acgacauacc auuaccuugg cacgcuggag cagacacagg cacaccccac uggaauaaua     1320

aagaggcuuu aguugaguuc aaagaugcuc acgcuaaaag acaaacagua guaguacuag     1380

guucacaaga aggugcagua cauacagcuu uagcaggagc acucgaagca gaaauggaug     1440

gagcaaaagg aagauuauca aguggacauc uaaagugcag gcuaaaaaug gauaaguuaa     1500

ggcuaaaagg aguaucauau ucucuaugca cagcagcauu uacauucaca aaaauaccag     1560

cagaaaccuu acauggcacc guaacaguag aaguacaaua ugcagguaca gauggcccuu     1620

guaaaguccc agcacaaaug gccguagaua ugcaaacauu aacaccagug ggcagauuaa     1680

uaacagcaaa ccccguaaua acugaaagua cagaaaauag uaaaaugaug cuagaauuag     1740

auccuccauu uggcgauagu uauauaguaa uaggaguagg agaaaagaaa auaacacauc     1800

acuggcaccg cagcggaagu acuauuggaa aagcauuuga agcaacugua agaggagcaa     1860

aaagaauggc uguucuagga gacacagcuu gggacuuugg uucuguagga ggagcacuaa     1920

auaguuuggg aaaaggcaua caucaaauuu uuggcgcugc auucaaauca cuauuuggag     1980

gaaugaguug guuuucacaa auucucauag gaacauuauu aauguggcua ggguuaaaca     2040

cgaaaaaugg aaguauauca uuaauguguu uagccuuagg uggaguauua aucuucuuau     2100

cuacagcagu aucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  6981
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6981
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgug       60

gagcugauac cucaguuggu auaguaggcu uacuauuaac aacagcaaug gcugccgcug      120

uuacuagaag aggaucagca uauuauaugu acuuagauag aaaugaugca ggagaagcaa      180

ucucauuucc cacuacuuua ggcaugaaua aauguuacau ucaaauaaug gauuuaggac      240

auauguguga ugcaacaaug ucauacgaau gcccaauguu agaugaaggc guagagcccg      300

augauguaga uuguuggugu aauacuacau cuacuugggu aguguaugga acaugucauc      360

acaaaaaagg agaagcaaga cgaagcagac gagcaguaac auugccaucu cacucaacaa      420

gaaaauuaca aacaagauca caaacauggc uagaaucuag agaauacaca aaacauuuaa      480

uaagaguaga aaauuggaua uuuagaaauc caggauucgc guuggcggcc gcagcgauag      540

caugguuauu aggaucaagu acaucacaaa aaguuaucua uuuaguaaug auccuacuaa      600

uagcaccagc guauaguauu agauguauag gcguaucaaa uagagauuuc guagagggga      660

ugaguggugg aacaugggua gauauaguuu uagaacaugg ugggugugua acgguaaugg      720

cucaagauaa accaaccgua gauauagagu uagucacaac uacagucagc aauauggccg      780

aaguaagauc auacuguuau gaagcaucaa ucucagauau ggccagugac aguagauguc      840

caacucaagg agaagcuuau cucgacaaac aaagugauac acaauaugua uguaaaagga      900

cacuagugga uagagguugg gguaaugguu guggacucuu uggcaaaggu agucuaguca      960

cgugcgcuaa auuugcuugc ucaaaaaaaa ugacgggaaa aagcauacaa ccugaaaauu     1020

uagaauaccg aauaauguua ucaguucaug gcagucaaca uagcgggaug auaguuaacg     1080

auacaggaca ugaaacagau gaaaauagag caaaagugga aauaacaccu aacucaccua     1140

gagcggaagc cacguuagga ggauuuggaa guuuaggauu agauugcgaa ccaaggacag     1200

gauuagauuu cucagauuua uauuaucuaa cuaugaauaa caaacacugg uuaguacaua     1260

aagagugguu ccacgauauu ccacuaccau ggcaugcugg ggcagacacu ggaacaccuc     1320

auuggaauaa uaaagaagca uuaguagaau ucaaagacgc acaugcuaaa agacaaacug     1380

uagugguacu aggaagucaa gaaggagcag uacauaccgc acuugcugga gcacuagaag     1440

cagaaaugga uggugcaaaa ggaagacuuu caaguggaca ucuaaaaugu agauuaaaaa     1500

uggacaaauu aagauuaaaa ggaguaucau acucauuaug uacagcagcc uuuacauuca     1560

caaaaauacc ugcagaaaca cuucauggaa caguaacagu agaaguacaa uaugcuggaa     1620

cugauggccc guguaaaguu cccgcucaaa uggcaguuga uaugcaaaca uuaaccccug     1680

ucgggagauu gauaacagcu aauccaguua uaacagaauc uacugaaaac ucaaaaauga     1740

uguuagaauu agacccacca uucggagaua gcuauauagu aaucggggua ggugaaaaaa     1800

aaauuacaca ccauuggcac agaaguggau cuacaauagg aaaagcauuu gaagccacug     1860

uuagaggagc aaaacgaaug gcaguacuag gugacaccgc augggauuuu ggaucagucg     1920

gcggugcauu aaacucacua ggaaaaggaa uccaucaaau auuuggagcu gcauuuaaaa     1980

gccuauuugg uggaauguca ugguuuagcc aaauacuaau uggaacuuua uuaauguggc     2040

uaggauuaaa uacaaaaaau ggaucaauua gcuuaaugug uuuagccuua ggaggaguuu     2100

uaauauucuu aagcacagcu guaagugcau gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  6982
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6982
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gacuaucuag cagaaaaaga agaucacaug acguauuaac cguucaguuc uuaauauuag      120

gaauguuauu aaugacagga ggaguaacaa gacguggcuc cgcguauuau auguaccuag      180

auagaaauga ugcaggagaa gcaauuucau uuccuacaac uuuaggaaug aauaaaugcu      240

auauacaaau cauggaccua ggacauaugu gcgaugccac aaugagcuau gaauguccaa      300

uguuagauga aggcguagaa ccagaugacg ucgauugcug guguaacaca acuucaaccu      360

ggguaguaua uggaaccugu caucauaaga aaggagaagc aagacgauca aggcgagcag      420

uaacauuacc aucucacuca acaagaaaau uacagacaag aucacaaaca uggcuagaaa      480

guagagaaua uacaaaacau uuaauaagag ucgaaaauug gaucuuucgu aauccugguu      540

uugcacuagc ugcagcugcu auagcuuggu uauuaggcuc aagcacauca caaaaaguua      600

uuuacuuagu aaugauauua cuaauagcac cagcuuacuc aauaagaugu auagguguuu      660

ccaacagaga uuuuguagaa gguaugucug gaggaacuug gguugauauc gucuuggagc      720

augguggaug uguaacggua auggcucaag auaaaccuac aguagauaua gagcuaguua      780

cgacaacagu uagcaauaug gcugaagucc gaaguuauug cuacgaagca agcauaagug      840

auauggcaag ugauucaaga ugcccaacac aaggagaagc auaucuagau aaacaaagug      900

acacacaaua cguuuguaaa agaacucuug uagauagggg uuggggcaau ggaugugguu      960

uauucggaaa aggaaguuua guaacaugug cuaaauuugc uuguucuaag aaaaugacug     1020

gcaaaucaau ucaaccagaa aauuuagaau acagaauaau gcuaucaguu caugguucac     1080

aacauucugg aaugauagua aaugauacag gacaugaaac agaugaaaau cgugcuaaag     1140

uagaaauuac accaaacuca ccaagagcag aagcaacucu ugguggauuu ggcucauugg     1200

gguuggauug cgaaccaaga acuggauuag auuucaguga uuuguauuau uuaacuauga     1260

auaauaaaca cuggcuaguu cauaaagagu gguuccacga cauaccauua ccauggcacg     1320

caggagcaga uacaggaaca ccacauugga acaauaaaga agcacucguu gaauuuaaag     1380

augcacaugc aaaaagacaa accgugguag uauugggauc ucaagaagga gcaguucaca     1440

cagcacuagc aggagcauua gaagccgaaa uggacggagc aaaaggaagg uuaucuucag     1500

ggcaucuaaa auguagauua aaaauggaca aacuaagauu aaaaggggua aguuauucac     1560

uauguacagc agcuuuuaca uucacaaaaa uaccugcaga aacacugcac gggacaguaa     1620

caguagaagu acaauaugca ggaacagaug guccauguaa gguacccgca caaauggcag     1680

uugacaugca aacucuaacg ccaguaggaa gacuaaucac agcuaaucca guaauaacag     1740

aaucaacuga aaacucaaaa augaugcuag agcucgaucc accuuucgga gauucauaca     1800

uugugauagg uguuggagaa aagaaaauua cacaucauug gcauagauca ggaucaacua     1860

uaggaaaagc auuugaagcc accguaagag gagcaaaaag aauggcugug cuaggagaua     1920

cugccuggga uuuuggaagu guaggaggag cauuaaauuc acuagggaaa ggaauacauc     1980

aaauauuugg agcagcauuu aaaucacuau uuggugguau gagcugguuu ucacaaauuc     2040

uaauaggaac auuauuaaug ugguuagguc uaaacacuaa aaacggaagc auaucuuuaa     2100

ugugccuagc acuagguggg guacucauau uucuaagcac ugcugugagu gcaugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6983
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  6983
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gguuaucaag ucgaaaaagg agaucacaug auguuuuaac aguacaauuu uuaauuuugg      120

gcaugcuacu uaugacugga gcaguaacaa ggagaggaag ugccuauuau auguaucuug      180

auagaaauga ugcaggagaa gcaauuucau uuccaacaac auuaggaaug aauaaauguu      240

auauacaaau aauggauuua ggacacaugu gugaugcaac aaugucauac gaauguccaa      300

ugcuagauga agguguggaa ccugaugaug uagauugcug guguaacaca acaagcacuu      360

ggguaguaua cggaacaugu caccacaaga aaggggaagc aagaagaagu agaagggcag      420

uuacccuacc aagccauuca acaagaaaau uacaaacaag aucacaaaca uggcuagaaa      480

guagagaaua cacaaaacac uuaauaagag uagaaaacug gaucuuuaga aauccaggau      540

ucgcacuagc agcugcugcu auugcuuggc uauuaggaag caguacuagc caaaagguaa      600

uauaucuugu uaugauauua cuaauagcac cagcauacuc aauaaggugc auaggaguau      660

caaauagaga uuuuguagaa ggaauguccg gagguacaug gguggauauu guacuagaac      720

auggcggcug cgucacagug auggcacaag acaaaccaac aguagauaua gaauuaguua      780

caacaacagu cucgaauaug gcagaaguaa gaaguuauug uuacgaagca agcauaagug      840

auauggcaag cgacagucgg ugcccuacuc agggagaagc auauuuagau aaacaaucag      900

auacccaaua cguauguaaa cgaacucuag uagacagagg guggggaaau ggauguggac      960

uauuugguaa aggaucacug guaacaugcg caaaauuugc augcaguaag aaaaugacag     1020

gcaaaaguau ucaaccugaa aauuuggaau auagaauaau gcuuagcgua cauggaucac     1080

aacacucagg aaugauagug aaugauacag gacacgaaac agaugaaaau agagcaaaag     1140

uagaaauaac accuaauagu ccuagagcgg aagcaacacu aggaggauuc ggaucauugg     1200

gucuugacug ugaaccacga acuggguuag auuuuucaga ucuauauuau uuaacaauga     1260

auaauaaaca uugguuagua cacaaagagu gguuccauga uauaccauug ccauggcaug     1320

caggagcgga uacuggaaca ccgcauugga acaacaaaga agcacuugua gaauuuaaag     1380

augcacaugc aaaaaggcaa acaguaguag uacuuggauc ucaagaagga gcaguacaua     1440

cugcuuuagc aggagcauua gaagcugaaa uggauggagc aaaagguaga cuaagcucgg     1500

gacacuuaaa augcagacua aagauggaua aauuaagauu aaaaggugug ucguacaguu     1560

uauguacagc ugcauuuacc uuuacuaaaa uaccagcaga aacauuacau ggcacuguaa     1620

caguugaagu acaauaugca ggaaccgaug guccaugcaa aguaccagcu caaauggcag     1680

uagauaugca aacgcuuacu ccaguaggaa gguuaauaac agcuaaucca guaauaacug     1740

aauccaccga aaauagcaaa augauguuag aauuagaucc accauuuggg gauucauaua     1800

uaguuauagg aguaggagaa aagaaaauaa cucaccauug gcacagauca ggaaguacaa     1860

uaggaaaagc guucgaagca acaguaaggg gagcaaaacg aauggcagua uuaggagaca     1920

cagcauggga cuuugguuca guaggcggag cauugaauag cuuaggaaaa ggaauacacc     1980

agauauuugg cgcagcuuuc aagagcuuau uuggaggaau gucgugguuu agccaaauac     2040

uaauaggaac uuuauugaug uggcuaggac ugaacacuaa aaacggaagu auuucauuaa     2100

uguguuuagc auuaggagga guauuaaucu uuuuaucaac agcaguaagu gcuugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  6984
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 2 opt

<400>  6984
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgua       60

uaauaaaugc aagaaaagaa aagaaaagaa gaggcgcaga uacaagcgua ggcauaguug      120

gguuacuguu aacaacagcu auggcugcag aaguaacaag aagagguagc gcuuauuaca      180

uguauuuaga caggaaugac gcuggagaag caauuagcuu uccaacaacu uuaggaauga      240

auaaauguua uauucaaaua auggaucuag gacacaugug cgacgcaacc augaguuaug      300

aauguccaau gcuagacgaa ggaguagaac ccgaugaugu ugauuguugg uguaauacaa      360

cgucaaccug gguuguauau ggcacauguc aucauaagaa aggagaagca agaagaagua      420

ggagagcagu aacacuaccu ucacauucaa cuagaaaacu acaaacucgu agccaaacau      480

ggcuagaauc acgagaauau acaaaacauc ugauaagagu agaaaauugg auuuuuagaa      540

auccuggauu ugcucuagcu gcagccgcaa uagcauggcu acuaggaucu aguacuagcc      600

aaaaagugau auacuuagua augauauuac uaauagcucc ugccuacagu auacgcugca      660

uaggaguaag caauagggac uucguagagg gaaugucagg aggcacaugg guagauauag      720

uacuagaaca cggaggaugu guaacaguaa uggcccagga uaaaccaacu guagauauug      780

agcuaguaac uacaacugua ucaaacaugg cugaagucag aaguuacugu uacgaagcaa      840

guauaaguga uauggccuca gauucacgau guccaacuca aggagaagcc uaucuagaua      900

aacaaucaga uacucaauau guauguaaaa gaacuuuagu agauagaggc uggggaaaug      960

gcuguggauu auuuggaaaa ggcagcuuag ucacaugcgc aaaauuugcc ugcagcaaga     1020

aaaugacggg aaaaucgaua caaccggaaa auuuagaaua uagaauaaug uuaucaguac     1080

acgggagcca acauucagga augauaguaa augauacagg acacgaaacg gaugaaaaua     1140

gagccaaagu ugaaauaaca ccuaacaguc caagagcaga agcuacacua gguggauucg     1200

gcucauuagg auuggauugu gaaccacgaa cuggcuuaga cuucucugac uuauacuacu     1260

uaacuaugaa uaauaaacau uggcuaguac auaaagaaug guuccaugac aucccuuuac     1320

cauggcaugc aggagcagau acaggaacac cucauuggaa uaauaaagaa gcacuuguag     1380

aguucaaaga ugcacaugcu aaaagacaaa caguaguagu auuaggauca caagaaggug     1440

ccguacacac ugcgcuagcu ggugcauuag aagcggaaau ggauggagcc aaaggaagac     1500

uaucuuccgg acaucuuaaa uguagauuaa aaauggauaa acuaagauua aaaggaguaa     1560

gcuauaguuu augcacagca gcuuuuacau uuacaaaaau accggcagaa acauuacaug     1620

gaacaguaac aguugaaguu caauaugcgg gaacggaugg accuugcaaa gucccagccc     1680

aaauggcugu ggacaugcaa acauuaacgc caguaggaag acucauuacc gcaaauccag     1740

uaauaacuga aucaacggaa aacaguaaaa ugaugcuaga acuagacccu ccauuuggag     1800

acagcuauau aguaauagga guaggagaaa agaaaauaac acaucauugg caucgaucug     1860

gaaguaccau cggaaaagca uuugaagcga caguacgagg agccaaaaga auggccguau     1920

uaggcgauac agcuugggau uucgguucgg ucggaggcgc auuaaauagu uuaggaaaag     1980

gaauacauca aauuuucgga gcagcauuca aaagccuauu ugggggcaug agcugguuuu     2040

cacaaauacu caucgguacu uuauuaaugu ggcuaggacu caauacgaaa aaugguagua     2100

ucucauuaau guguuuagca uuaggcggag uauugauuuu ucuuaguaca gcaguaagug     2160

ccgacguagg augcucagua gauuucagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6985
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 2 opt

<400>  6985
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaacgc uagaaaagag aaaaagcgua gaggagcuga uacaagcguu gggauaguag      120

guuuguuacu aacaacagca auggcagcag aaguuacaag aagagggagu gcauauuaca      180

uguaucuaga caggaacgau gcuggugaag caauuucauu cccaacaaca uuaggaauga      240

auaaauguua uauacaaaua auggacuuag gacacacaug cgaugcaaca augucauaug      300

aguguccgau guuagaugag ggaguagaac cagaugacgu agauuguugg uguaauacaa      360

caaguacaug ggucguguau ggaacaugcc accauaagaa aggagaagca agaagaagua      420

gacgagcagu aacauuacca agucacagua caagaaaacu acaaacuaga agucaaacau      480

gguuagaauc uagagaguau acaaaacacu uaaucagagu agaaaauugg auauuuagaa      540

auccaggauu ugcauuagca gcugcggcaa uagcuuggcu auuaggaagc ucaacaucuc      600

aaaaaguaau auauuuagua augauacuau uaauagcacc agcuuauagc auaaggugua      660

uuggaguauc aaauagagau uucguugaag gaaugucagg aggaacaugg guagauguug      720

uucuagaaca uggagguugc guuacaguca uggcacaaga uaaaccaaca guagauauag      780

aauuagucac aacaacaguu aguaacaugg cagaaguacg cucauauugu uaugaagcaa      840

gcauaagcga uauggcauca gauucaagau guccaacaca aggagaagca uauuuagaua      900

aacaaucuga cacacaauau guauguaaac guacguuagu agaccgugga ugggggaacg      960

gauguggauu auuugguaaa ggaucguuag uaacaugcgc aaaauucgca ugcaguaaga     1020

aaaugaccgg aaaaagcaua caacccgaga acuuagaaua uagaauaaug uuaagcguac     1080

auggcagcca acauagugga augaucguca acgauacagg acaugaaaca gaugaaaaua     1140

gagcuaaagu agaaauaacc ccaaacagcc caagagcaga agcaacauua gguggauuug     1200

guucacuagg auuagauugc gaaccuagaa cgggccugga uuuuagugac cuauacuauu     1260

uaacaaugaa uaauaaacac ugguuaguuc auaaagaaug guuucaugau auuccucuac     1320

cauggcaugc aggcgcagau acagguacac cacacuggaa caauaaagaa gcauuaguag     1380

aguucaaaga ugcacacgca aagagacaaa cugucguggu acuagguagu caagaaggug     1440

caguucauac agcacucgca ggagcguuag aagcagaaau ggauggagcg aaaggaagau     1500

ugaguucagg ucaucuaaaa ugccgacuaa aaauggauaa auuaagguua aaagguguau     1560

cauauucauu augcacagcc gcuuucacau uuacaaaaau cccagcagaa acuuuacaug     1620

gaacaguaac uguagaagug caauaugccg gaaccgaugg accaugcaaa gucccugcuc     1680

aaauggcugu agacaugcaa acauuaacgc caguuggaag acuaauaaca gcaaaccccg     1740

uaauaacgga aagcaccgaa aacagcaaaa ugaugcuaga auuagaucca ccuuuuggag     1800

acaguuauau cguuauaggc guaggcgaaa agaaaauuac ccaucauugg cauagaagug     1860

gaagcacaau uggcaaagca uucgaggcaa caguaagagg cgccaaaaga auggcugucu     1920

uaggagacac agcaugggau uuuggaagug ucggaggagc auuaaauagc cuuggaaaag     1980

gaauacauca aauauuuggg gcagcauuca aaucacuauu uggaggaaug agcugguuuu     2040

cacaaauuuu aauagguacc cuauuaaugu gguuaggacu aaaugcuaaa aauggaagua     2100

uaaguuuaau gugucuugca uuaggaggag uauuaauauu ucuaucaacg gcuguaucag     2160

cugauguagg auguagugua gacuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6986
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 2 opt

<400>  6986
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaacgc ucgaaaagaa cgaaaaagaa gaggagcaga uacuaguauu ggcauaauag      120

gucuauuacu uacuaccgcu auggcagcug aaauaacaag aagaggaagc gcauacuaca      180

uguauuuaga uagaagugau gcaggaaaag caauuucuuu ugcaacgaca uugggaguaa      240

auaaaugcca cguucaaaua auggaucuag gacauaugug ugaugcuacu augucauacg      300

aauguccgau guuagaugaa ggaguagaac cagacgacgu cgauuguugg uguaauacaa      360

ccagcacaug gguaguguac ggaacaugcc accauaaaaa aggagaggca agacgaagca      420

ggcgagcagu aacauugccg agucacucaa caagaaaacu acaaacuagg ucacaaacau      480

gguuagaaag uagagaauau acaaaacauu uaauaaaagu ggaaaauugg auauuuagaa      540

auccaggauu cgcucuaguu gcuguggcaa uagcuugguu auuagguagu aguacuaguc      600

aaaaagucau auaucuaguu augauucuac ugauagcacc agccuauucu auaagaugua      660

uaggaguauc uaacagagac uuuguagaag guaugucggg aggaacuugg guagauguag      720

uacuagaaca uggagguugu guaacgguca uggcacaaga uaaaccuacu guagacauag      780

aauuaguaac aaccaccgua ucgaacaugg cagaaguaag aucuuauugc uaugaagcaa      840

guauuagcga uauggcaagc gacucaagau gcccuacuca aggagaagca uacuuagaca      900

aacaguccga uacucaauau guguguaaaa gaacuuuagu agauagagga uggggaaacg      960

gcuguggacu auuuggcaaa ggaucauuag ugacaugugc aaaauuuaca uguagcaaaa     1020

agaugacagg uaaaaguaua caaccagaaa auuuagaaua cagaauaaug uuaaguguac     1080

auggcucuca acauagcgga augauaggau augaaacaga ugaggauaga gcaaaaguag     1140

aaguaacacc uaauaguccu cgugcagaag caacucuggg uggauucgga agccuaggau     1200

uggauuguga accacgaaca ggauuagauu uuagugaucu auauuacuua acaaugaaua     1260

auaaacauug gcuaguccau aaagaauggu uucaugacau uccauuacca uggcacgcag     1320

gugcagauac cggaacacca cacuggaaua auaaagaagc uuuaguagaa uuuaaagacg     1380

cgcaugcaaa gaggcaaacg gugguaguau ugggaucaca agagggagca guacauacag     1440

cucuagcagg agcacuugaa gcagagaugg acggugcaaa aggaagauua uuuucaggac     1500

acuuaaaaug uagacucaaa auggauaaau uaagguuaaa aggagucagc uauagcuuau     1560

gcacagcagc auuuacauuc acaaaaguac cagcagagac auuacauggg acaguuacag     1620

uugaaguaca auaugcggga acagauggac cauguaaaau acccgugcaa auggcaguug     1680

acaugcaaac auuaacaccu guaggaagau uaauuacagc gaacccagua auaacagaau     1740

cuacagaaaa uaguaaaaug auguuagaac uagacccacc auuuggagau aguuauauag     1800

ugauaggagu gggugauaaa aaaaucacac aucacuggca uaggagugga ucuacuauug     1860

gaaaagcuuu ugaagcaaca guaagaggag caaaaagaau ggcaguuuua ggagauacag     1920

caugggauuu cggaagcgua ggagggguau uuaauagccu agggaaaggu auucaucaaa     1980

uauuuggagc cgcauuuaaa agucuauucg guggaaugag uugguucuca cagauauuaa     2040

uaggaacauu auuagucugg uuaggauuaa auacaaaaaa ugggucaaua ucucugacau     2100

gucuagcacu agguggagua augauauuuc uaaguacagc aguaagcgca gaugucggau     2160

guagcguaga cuuuucgaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6987
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 2 opt

<400>  6987
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc gagaaaggaa aaaaaacgac gaggagcaga uacaucaguu ggaauaguag      120

gcuuauuauu aacaacggca auggccgcag aagugacaag aagaggaagu gcauauuaca      180

uguauuuaga uagaaacgau gcuggagaag cuauaucuuu uccaaccaca uuaggaauga      240

auaaauguua cauacaaaua auggauuuag gucauaugug ugaugcuaca augucauaug      300

aguguccaau guuagaugaa gguguagaac cugaugacgu agauuguugg ugcaacacaa      360

caaguacaug gguaguauau ggaacauguc aucauaaaaa aggagaagcc agaagaagca      420

gacgugcugu aacauuaccc ucacauagca caagaaaacu acaaacccga agucaaacgu      480

gguuagaauc uagagaauac acaaaacauc uuauaagagu agagaauugg auauuuagaa      540

auccugguuu cgcauuggca gcagcagcaa uugcguggcu acuaggaucg ucuacaaguc      600

aaaaaguaau auacuuagua augauauuac uuauagcgcc ugcauauagu auaagaugua      660

uaggaguaag caauagagau uuuguagaag guaugucagg ggguacgugg guagauguag      720

uauuagaaca ugguggaugc gucacaguaa uggcacaaga caaaccuaca guagauauug      780

aacuaguaac aacaacagua ucaaauaugg ccgaaguaag aucauauugu uaugaagcau      840

cuauaaguga uauggcuagu gacaguagau guccaacaca aggugaagca uaucuagaua      900

aacaaagcga uacacaauau guguguaaaa gaacauuagu agauagagga ugggguaacg      960

gaugugguuu auuuggaaaa ggauccuuag uaacgugugc aaaauuugcu uguagcaaaa     1020

aaaugacagg aaaaucaauc caaccagaaa auuuagaaua uagaaucaug uuguccgugc     1080

augguaguca acacucugga augauuguaa augauacagg acaugaaacc gaugaaaaua     1140

gagcaaaagu ugaaauaaca ccgaauagcc caagggcaga agccacauua ggaggauuug     1200

gcucguuagg ucuagauugu gaaccaagaa ccggauuaga uuuuagugac cuauauuauc     1260

uaacuaugaa caacaaacac ugguuaguuc acaaagaaug guuucacgau auacccuuac     1320

ccuggcaugc uggggcagac acagguacac cacauuggaa uaauaaagaa gcacuuguag     1380

aauucaaaga cgcucaugcc aagagacaaa cuguaguugu gcucgguagu caggaaggug     1440

cuguacacac agcacuugca ggagcacuug aagcagaaau ggauggagca aaggguagau     1500

uaucuagugg ucauuuaaaa uguagauuaa aaauggacaa auuaagauua aaaggcguaa     1560

guuauucacu auguacugca gcauuuacau uuaccaaaau accagcagaa acacuacaug     1620

gaacugucac aguagaagua caguaugccg gaacagaugg uccauguaaa gugccggcuc     1680

aaauggcagu ugauaugcaa acauuaacac caguaggaag auuaauaaca gcaaauccgg     1740

uuauuacaga gucuacggaa aauucgaaaa ugaugcuuga acuagaccca ccauucggug     1800

acaguuacau ugucauuggu guaggugaaa aaaaaauaac acaccauugg cauagaucag     1860

gaaguacaau aggaaaagca uuugaagcua cagugagagg agcgaaacga auggcaguac     1920

ucggugauac agcaugggau uuuggaucug uagguggagc auuaaauagu uuaggaaaag     1980

guauacauca aauauuugga gcagcuuuua aaucacuauu uggaggaaug ucaugguuuu     2040

cucaaauucu cauagguaca cuauuaaugu ggcuaggccu aaauacaaaa aacggaagca     2100

uaaguuuaau guguuuagcu uuaggaggug uuuuaauuuu uuuaaguaca gcgguaucag     2160

cagauguagg auguucaguu gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6988
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 2 opt

<400>  6988
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagag cgaaaacgua gaggagcaga uacaagcauu ggaaucauag      120

guuuacuauu gacaacagca auggcugcug aaauaacuag aagaggcucc gccuauuaua      180

uguaccuaga caggucagac gcagguaaag ccauaagcuu ugcaacaaca uuaggcguaa      240

auaaauguca uguacaaaua auggauuuag gacauaugug ugacgcuaca augaguuaug      300

aauguccaau guuagaugaa ggaguggaac cagacgaugu ugauuguugg uguaauacca      360

cuaguacaug gguaguauau gguacguguc accacaaaaa aggcgaagca agaagaucca      420

gaagagcggu cacauuacca ucacauucaa cuagaaaacu acaaacaaga ucccaaacau      480

ggcuugaaag cagagaguac acuaaacacc uaauaaaagu agaaaauugg auauucagaa      540

acccuggauu ugcauuggua gcaguagcaa uagcaugguu auuaggaagu agcaccucac      600

aaaaaguaau auaucuagua augauucuac uaauugcacc agcauauuca auaagaugca      660

uagguguuuc caauagagau uuuguagaag gaaugucagg aggaacaugg guagacgucg      720

uccuagaaca cggugggugc guaacuguaa uggcacagga uaaaccaacu guagauauag      780

aauuaguaac aacaacugua ucuaauaugg cagaaguaag aucauauugu uacgaagcau      840

caauaucuga uauggcaagu gauuccagau gcccaacaca aggagaagca uauuuagaua      900

aacaaucaga uacacaauau guuuguaaaa gaacccuggu cgaucgaggu ugggguaaug      960

gauguggucu uuuugguaaa ggaagccuag uaacaugugc aaaauuuaca uguagcaaaa     1020

aaaugacagg aaaaucuaua caaccagaaa aucuugaaua uagaauaaug uuaaguguuc     1080

auggaagcca acauucaggc augauaguga augauacagg uuaugaaaca gaugaaaaca     1140

gggcgaaagu agaaguaaca ccaaacucac cacgagcaga agccacuuua ggaggauucg     1200

gaucauuagg acuagacugu gagccaagaa caggauugga uuuuagugau cuauacuauu     1260

uaacaaugaa uaauaaacac ugguuaguac acaaagaaug guuccaugac auaccauuac     1320

cuuggcacgc cggagcagac acaggaacac cccacuggaa uaauaaagaa gcgcuaguag     1380

aauuuaaaga ugcacacgcc aaaagacaaa cugucguagu auuaggauca caagaaggug     1440

caguacauac agcucucgca ggagcauuag aagcagaaau ggacggagcc aaagguaaac     1500

uuuuuucagg acauuuaaaa uguagauuaa aaauggauaa auugcgacua aaagguguaa     1560

gcuauucacu auguacagca gcauuuacau ucacaaaagu acccgcagaa acacuacaug     1620

gaaccgugac aguagaaguu caauaugcag gaacagaugg cccauguaaa auaccuguac     1680

aaauggcagu agauaugcaa acacuaacuc ccgucggcag auuaauuaca gcaaauccag     1740

uaauaacuga aaguacagaa aauucaaaaa ugaugcuaga auuagauccg ccauucggag     1800

auucauacau aguuauuggc gugggagaca aaaaaauuac acaucacugg cauagaagcg     1860

gaaguaccau aggaaaagcu uuugaagcaa caguucgagg agcaaaaaga auggcaguau     1920

uaggugacac ugccugggau uucgguuccg uaggaggagu uuucaauuca uuaggcaaag     1980

guauucacca aauauuuggu gcggcauuca aaucauuauu uggagguaug ucaugguuua     2040

gucaaauacu aauaggaaca uuacuaguau gguuaggauu aaauacaaaa aacgggucca     2100

uaucuuuaac auguuuagcu cuagguggcg uuaugauauu uuuaaguacu gcaguaagug     2160

cagauguugg cugcucaguc gauuuuucua agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6989
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 2 opt

<400>  6989
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa agaaaaagaa gaggggcuga uacaaguaua gguauaguag      120

gacugcuauu aacaacggca auggcugcag aaauaacaag gaggggauca gcauauuaca      180

uguaucuaga uagaucugau gcgggaaaag caauaucuuu ugcgacaacu uuaggaguaa      240

auaaaugcca uguacagaua auggauuuag gacauaugug cgaugcgacu augaguuaug      300

aaugcccaau guuagacgaa gggguugaac cagacgacgu cgauuguugg uguaauacaa      360

cgaguacuug gguaguauau ggaacaugcc aucacaagaa aggcgaagca agaagaagca      420

gacgagcggu aacauuacca ucacauucaa caagaaaacu acaaacaagg ucacaaacau      480

ggcuagaaag cagagaauau acaaaacauu uaaucaaagu agaaaauugg aucuucagaa      540

auccagguuu uacucuagua gccgucgcaa uugcaugguu auuaggaagu aguacaucac      600

aaaaaguaau auauuuagua augauacuau uaauagcgcc agcauauagu aucagaugua      660

uaggaguaag caauagagau uuuguagaag gaaugucagg agguacaugg guagacgugg      720

uacuugaaca uggagguugu guuacuguaa uggcacaaga uaagccgacu guagauauag      780

aauuaguuac aacaacugua aguaauaugg cagaaguucg aaguuauugu uacgaagcau      840

cgauaaguga uauggcauca gacucaagau guccuacaca aggugaagca uaucuagaca      900

aacaaucaga uacacaauac guauguaaaa gaacauuagu agaucguggg uggggaaaug      960

gaugcggauu guuuggaaaa ggaagccuag uaacaugcgc uaaauuuacc uguaguaaaa     1020

agaugacugg aaaaucaauu cagccggaaa accuagaaua cagaauaaug cuaucaguac     1080

acggaaguca gcauucaggu augaucguaa augacgaaaa uagagcuaaa guagaaguga     1140

cgccuaacag uccaagagca gaagcuacgc uaggagguuu ugggagucuc ggauuagauu     1200

gugaaccuag aacaggauug gauuuuagcg auuuauacua cuuaacuaug aauaauaaac     1260

auuggcuugu acauaaggaa ugguuucaug acauaccauu accauggcau gcaggagcag     1320

auacagguac accccauugg aauaauaaag aagcucuagu ugaguucaag gaugcacaug     1380

cuaaaagaca aacagucgua guauuggggu cacaagaagg ugcaguacau acagcucuug     1440

cuggagcacu agaagcugaa auggauggag cuaaaggaag acuguuuagu ggucauuuaa     1500

aaugucgauu aaaaauggac aaauuaagau ugaaaggggu aagcuacucu uuauguacug     1560

cagcauucac guucacuaaa guaccggcug aaacauuaca uggaacagua acaguagaag     1620

uacaauaugc ggguacagau ggaccaugua aaguaccagc ucaaauggcg guagauaugc     1680

aaacacuaac accaguuggg agauugauaa cagcgaaccc aguaauaacg gaaaguacag     1740

aaaauaguaa aaugauguug gaacuugacc caccauucgg agauaguuau auaguuaucg     1800

gaguaggaga uaagaaaauu acacaucauu ggcauagaag uggaaguaca auugggaaag     1860

cauuugaagc cacuguuaga ggggcaaaaa ggauggcagu uuuaggagau acagcauggg     1920

acuuuggaag cguaggcggu guuuuuaaua gucuagguaa agggauacau caaauauuug     1980

gugcagcauu caaaaguuua uuuggaggga ugucaugguu cagucaaauu cuaauuggua     2040

cacuauuagu augguuaggu cuaaauacga aaaauggaag uauuucauug acaugucuug     2100

cauuaggugg aguaaugauu uuccuaagua cagcuguuag ugcagaugug ggauguagug     2160

uagauuuuuc aaaaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  6990
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 2 opt

<400>  6990
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc uagaaaagaa agaaaaagaa gaggagcaga uacauccaua ggaauaauag      120

gauuacuucu cacaacagcg auggcagcag aaaucacaag acgcggaagu gcauacuaua      180

uguaucuaga uagauccgau gcaggaaagg cgauaucauu ugcaacgaca uuaggcguua      240

auaaauguca uguacaaauu auggauuugg gccacaugug ugaugcuacc augaguuacg      300

aauguccuau gcuagaugaa ggaguagaac cugaugaugu ggauuguugg ugcaacacaa      360

caucaacaug ggugguuuau gguacuugcc acagaaagaa aggugaagca agaagaagca      420

gacgagcagu aacacuucca ucccauucaa cacggaaacu acaaacaaga ucacaaacau      480

gguuagaauc uagagaauac acaaaacauc uuauaaaagu agaaaauugg auuuuuagaa      540

acccaggauu ugcauuagua gcuguugcua uagcauggcu ucuagguuca ucaacaucac      600

aaaaaguaau uuauuuaguu augauauuau uaauagcucc agcauauagu auuagaugua      660

uuggaguauc gaauagagau uuuguugaag guaugagugg aggcacaugg guggacgucg      720

uauuagaaca cgggggaugu guaacaguaa uggcacaaga caaaccaaca guugacauag      780

agcuaguaac uacaacagua aguaacaugg cagaaguaag aagcuauugu uaugaagcca      840

guaucaguga cauggcaagu gacaguagau guccuacaca aggagaggcu uauuuagaua      900

aacaaaguga uacacaguau guauguaaaa gaacauuagu agauagaggu uggggaaaug      960

gcuguggacu cuuuggaaaa ggaucgcuag uaacaugugc aaaauuuaca uguucaaaga     1020

aaaugacugg aaaaucaauu caaccagaaa acuuggaaua ccgaauaaug cuaagcgugc     1080

auggauccca acauagcggg augauaguaa acgauacagg auaugaaacu gacgaaaaca     1140

gagcaaaagu agaaguaacu ccaaauaguc caagagcaga agcaaccuua ggaggauuug     1200

guucuuuagg acuagauugc gaacccagaa caggauuaga cuuuagugac cuuuauuauu     1260

uaaccaugaa uaacaaacau uggcuaguuc auaaagaaug guuucaugau auaccauuac     1320

cauggcaugc aggcgcagau acuggcacuc cacauuggaa caauaaagaa gcgcuaguag     1380

aauuuaaaga cgcucaugcc aagagacaaa caguggucgu acuuggaagc caagaaggcg     1440

cagugcauac agcucuagca ggagcacuag aagcagaaau ggauggugca aaaggaaagu     1500

uauuuagugg acauuuaaaa uguagacuaa aaauggauaa auuaagauua aaaggaguca     1560

gcuacucgcu cugcaccgca gcauuuacau uuacaaaagu accagcagaa acauuacaug     1620

gaacaguuac aguugaagua caauaugcag guacagaugg accauguaaa auaccaguuc     1680

aaauggcugu agacaugcaa acauuaacgc cuguagggag acuaauaaca gcaaauccag     1740

ucauaacgga aucaacagaa aacaguaaaa ugaugcuaga acucgacccu ccauucggag     1800

acucauacau aguaauugga guuggagaca agaaaauuac ucaccauugg cacagaucag     1860

ggucaacaau aggaaaagcc uucgaagcaa cuguaagagg agcuaaaaga auggcuguau     1920

uaggcgacac agcaugggau uuuggauccg ucggaggagu auuuaauagc uuaggaaaag     1980

gaauacacca aauauuugga gcggcuuuua aaucacuuuu uggcggaaug ucaugguuuu     2040

cacaaauacu uauaggaacu cuauuaguau gguuaggccu aaauacuaaa aauggaagua     2100

uaucauuaac augucuagca uuaggaggag ucaugauauu cuuaagcaca gcaguaagug     2160

ccgacguagg augcucagua gauuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6991
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 2 opt

<400>  6991
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa agaaaaagga gaggugccga uacgucuaua gguauaauag      120

gauuauuacu aacuacagca auggcggcag aaauaacacg aagaggaagu gcguacuaca      180

uguaucuaga cagaagugac gcaggaaaag caauuagcuu cgcaacaaca cuugguguaa      240

auaaauguca uguccaaaua auggauuuag gacauaugug ugaugccaca augucauaug      300

aauguccaau gcuagaugaa ggaguggagc ccgaugacgu ugacuguugg uguaauacaa      360

caagcacuug gguaguauau gguacuugcc aucauaagaa aggugaagcc agaagaagca      420

gacgagcagu uacauuacca ucacacagca cuagaaaauu acaaacaagg agucagacuu      480

gguuagaaag ucgagaauac acaaaacacc uaauuaaagu agaaaauugg auauuuagaa      540

acccuggcuu cgcauuagua gcaguugcaa uagcauggcu acuagguagu aguacaaguc      600

agaaaguaau uuauuuaguc augauccuau ugauagcacc agcauauuca auaagaugua      660

uugggguuuc aaauagagau uucguagagg ggaugagugg cggaacaugg guagacguag      720

uauuagaaca uggaggcugu guuacgguaa uggcgcaaga uaaaccaacu gucgauauag      780

agcuaguaac aacaacagua ucaaauaugg cagagguaag aucuuacugu uaugaggcaa      840

guauaaguga uauggcauca gacaguagau guccuacaca aggagaagca uauuuagaua      900

aacaaucgga uacacaauau guauguaaaa ggacgcuagu agaucgagga uggggaaacg      960

gauguggauu auucggaaaa ggaaguuuag uuacaugcgc aaaauucaca uguucaaaga     1020

agaugacagg aaaaucaaua caaccagaaa accuagaaua cagaauaaug uuaucuguac     1080

acggaaguca gcauucagga augacaguaa augacauagg auacgagacu gaugaaaaua     1140

gagcuaaagu agaaguaacu ccaaauucac cgagagcaga agcuacauua ggaggauuug     1200

gaagucuagg acuagacugu gaaccucgaa cagguuuaga uuuuagugau uuauauuacu     1260

uaacaaugaa caacaaacac uggcuaguuc auaaagaaug guuucaugac auaccauuac     1320

ccuggcacgc uggugccgau acaggaaccc cacauuggaa caauaaagaa gcauuaguag     1380

aauuuaaaga ugcacaugca aaaagacaaa caguaguagu cuuggguuca caagaaggcg     1440

ccguacauac ggcauuagca ggggcuuuag aagcagagau ggauggcgca aaagguaaau     1500

uauuuucggg acaccuaaaa ugcagauuaa aaauggauaa auuacgauua aaaggaguau     1560

cauauucacu auguacagca gcauuuacau uuacaaaagu accagcugaa acucugcacg     1620

gaacagugac gguagaaguu caauacgccg guacagaugg accaugcaaa auaccuguac     1680

aaauggcugu agauaugcaa accuuaacac caguaggaag auuaauuacu gcaaauccag     1740

uaauuacaga gagcaccgag aauagcaaaa ugaugcuaga auuagaucca ccuuuuggug     1800

auucauauau aguaaucggu guuggagaua agaaaauaac acaccacugg cauagaucag     1860

gaucaacaau agguaaagca uucgaagcaa caguaagagg cgcaaaacga auggcuguac     1920

uaggugauac agcuugggau uuuggaucag uaggaggagu auuuaacagu cuaggaaaag     1980

gaauacauca gauuuuuggg gcagcauuca aaaguuuauu uggaggcaug agcugguuca     2040

gucaaauauu aauuggaaca cuuuuaguau ggcuaggauu aaauacgaaa aaugguagua     2100

uaagucugac augucuagca uuagguggug uaaugauauu ucuuagcaca gcggucagcg     2160

cagauguagg auguucugug gacuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6992
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 2 opt

<400>  6992
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaauaaaugc cagaaaagaa agaaaaagga gaggugcaga uacauccaua ggaauaauag      120

gacuguugcu aacgaccgca auggcagcag aaauuacaag gaggggcuca gcauauuaua      180

uguaucuaga uaggucugau gcaggaaaag caauaaguuu ugcaacaaca cuagggguaa      240

auaaaugcca uguucaaaua auggauuuag gacauaugug ugaugcaaca augaguuacg      300

aaugcccuau gcuagacgaa ggaguggaac cggacgacgu cgauugcugg uguaauacaa      360

cuucaacaug gguuguauau gguacuugcc aucauaaaaa aggagaagca agaagaagcc      420

gaagagcagu aacacuacca agccauagca cucgaaaauu gcaaacaaga ucucagacau      480

ggcuagaauc aagagaauau acaaaacauu uaaucaaagu agaaaauugg auauuuagaa      540

auccaggauu cgcccuagua gcaguggcaa uagcaugguu auuaggaucu ucaacaaguc      600

aaaaagucau auauuuagug augauuuugc uaauagcucc agcauauucu auaagaugua      660

uaggaguauc aaacagagac uuuguugaag gaaugagugg cggaacaugg guagauguag      720

uauuagaaca uggaggaugu gugacaguaa uggcucaaga uaaaccaacu guugauauug      780

aacuuguaac aacaacagua ucuaacaugg cugaaguaag aaguuauugu uaugaggcuu      840

cuauaucaga uauggccuca gauaguaggu gcccuacaca aggagaagca uacuuagaua      900

aacaaaguga cacacaauac guauguaaac gaacuuuagu agauagaggu uggggaaaug      960

gcuguggucu auuuggaaaa ggaagucuag uaacaugugc aaaauuuaca uguucaaaaa     1020

aaaugacugg aaaaucaauu caaccagaaa auuuagagua uagaauaaug cuaucaguac     1080

auggaaguca acacaguggg augauaggau acgaaacaga cgaaaauagg gccaaaguag     1140

aaguaacacc gaacucucca agagcggaag cgacauuagg gggauucgga ucauuaggau     1200

uagauuguga accaagaaca ggauuagauu uuagcgaucu auauuacuua acaaugaaua     1260

acaaacauug gcuaguacau aaagaauggu uucaugauau accucuacca uggcaugcag     1320

gagcagauac ugguacgcca cauuggaaca auaaagaagc auugguagaa uuuaaagaug     1380

cacaugcuaa gaggcaaacg guuguaguac ucggaucaca agaaggagca guacauacug     1440

ccuuagcugg ugcacuagaa gcagaaaugg auggugcaaa aggaaaacua uuuaguggac     1500

auuuaaaaug ucgacuaaaa auggacaaac uaagacuaaa aggaguuuca uauucuuuau     1560

gcacagccgc auuuacauuc acaaaagucc cagcagaaac acuucacgga acagugacag     1620

uugaaguaca auaugcagga acggauggac cguguaaaau accuguacaa auggcaguag     1680

auaugcaaac acuaacacca guaggaagau ugauaacagc aaacccagua auaacagaau     1740

caacagaaaa cucaaaaaug auguuagagu uggauccgcc auucggagau aguuauauug     1800

uuauaggagu aggcgacaag aaaauaacuc aucauuggca uagaucagga agcacuauag     1860

guaaagcguu cgaggcaacg gugagagggg cuaaaagaau ggcaguauua ggagauacag     1920

caugggauuu uggaagugua ggaggaguau ucaacucacu aggaaaagga auacaucaaa     1980

uauuuggagc agcuuuuaaa ucacuauucg guggcaugag cugguuuuca caaauauuaa     2040

uaggcacacu auuaguaugg uuaggacuaa acacaaaaaa uggaaguaua agucuaacau     2100

guuuagcacu aggaggagua augauauuuc uaucaacagc agugagugcu gauguagguu     2160

gcaguguuga uuucucgaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  6993
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 2 opt

<400>  6993
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa aagaaaagac guggagcuga uacaucagua ggaauaguag      120

gauuacuauu aacaacugcg auggccgcag agguaacacg aagaggauca gccuacuaua      180

uguaucugga cagaaaugau gcaggagaag caauaaguuu uccaacuaca uuagguauga      240

auaaauguua uauacaaauu auggacuuag gacauaugug cgaugcuaca augucauacg      300

aaugcccaau guuagaugaa gguguagaac ccgacgaugu ugauuguugg uguaacacaa      360

caaguacaug gguaguuuac gggaccuguc accauaagaa aggagaagcg agaagaucaa      420

ggagagcagu cacucugcca agccacucua caagaaaauu gcaaacaaga agucaaacau      480

ggcuagaaag uagggaauac acaaaacauu uaaucagagu agaaaacugg auauuuagga      540

auccaggcuu ugcacuagca gcagcagcaa ucgcuugguu gcuagguagc aguacaaguc      600

agaaaguaau auauuuagua augauuuuau uaauagcacc agccuauuca auucgaugua      660

uaggugucag caauagagau uuuguagaag gaaugucugg cggaacaugg guugauguag      720

uacuugaaca uggaggaugu gugacaguaa uggcucaaga caaaccaacu guagacauag      780

aauuaguaac cacuacugua aguaauaugg cagaaguaag aaguuauugu uaugaggcuu      840

caauaucaga uauggcauca gacucaagau guccaacaca aggagaagca uaucuugaca      900

aacaaucaga uacgcaauau guauguaaaa gaacucuagu cgauagagga ugggguaacg      960

gauguggauu auucggaaaa gguagcuugg uaacuugugc aaaauuugca uguaguaaga     1020

aaaugacagg gaaaaguaua caacccgaaa aucuagaaua ucgaauaaug uuaagugugc     1080

auggaucgca acauagugga augauaguaa augauacagg acaugaaaca gaugaaaaua     1140

gagcuaaagu agaaauaaca ccaaauucac cgagagcaga agcaaccuua ggaggguuug     1200

gaaguuuggg auuagauugu gaacccagaa caggcuuaga cuuuucagau cuauauuacc     1260

uaacuaugaa caauaaacau ugguuaguac auaaagaaug guuucaugau auaccuuuac     1320

cauggcaugc aggagcagau acugguacac cgcauuggaa uaauaaagaa gcauuagucg     1380

aauuuaagga ugcacacgca aaaagacaaa caguaguagu acuaggaucu caggaaggcg     1440

cagugcauac agcacucgcu ggugcauuag aagcagaaau ggauggagca aaaggaagau     1500

uaagcucggg acaucuaaaa uguagauuaa aaauggacaa auugagauua aaagguguaa     1560

gcuacaguuu augcacagcc gccuucacau ucacaaaaau accugcagaa acauuacaug     1620

gaaccguuac gguagaagua caauacgcag gaacagaugg accauguaaa gucccagcac     1680

aaauggcagu agauaugcaa acauuaacac caguagguag auugauaacu gcaaauccag     1740

uaauuacgga aucgacagaa aauaguaaaa ugaugcuaga auuagaucca ccauucggcg     1800

auaguuauau aguaauagga guuggagaga aaaagauaac acaucacugg caucgaagug     1860

guucaacaau aggcaaagca uucgaagcaa cagucagagg agcaaaaaga auggcuguau     1920

uaggcgacac agcgugggac uucggaagug uagguggagc acuaaauuca uuaggaagag     1980

gaauacauca aauauuugga gcagcauuua aaucacuauu uggagguaug agcugguuua     2040

gucaaauauu aauuggaaca uuauuaaugu gguuaggacu aaauacuaaa aaugguucua     2100

uaaguuuaau guguuuagca cuaggaggag uacuaauuuu ucucaguaca gcuguaucag     2160

cagauguugg uuguucaguu gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6994
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 2 opt

<400>  6994
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc acgaaaagag aaaaagagaa gaggcgcaga ugccagcgua ggaauaguag      120

gauuacuacu aacaacugca auggcagccg aagucacucg aagaggaagu gcauauuaua      180

uguauuuaga uaggaaugau gcaggggaag caauaucauu ccccacaaca uuaggaauga      240

auaaaugcua cauacaaaua auggauuuag gacauaugug ugacgcaacg augucauacg      300

aauguccuau guuagacgag ggaguugaac cagaugacgu agauugcugg ugcaauacaa      360

caucaacaug gguuguauau ggcacuuguc accauaagaa aggagaagcg agaagaucua      420

ggagagcagu aacacuacca ucacauagua cgagaaaacu acaaacaaga ucucaaacau      480

gguuagaauc aagagaauau acaaaacacu uaauaagagu agaaaacugg auauucagaa      540

acccaggauu cgcauuagca gcagcagcaa uugcgugguu acuaggaagu aguacaagcc      600

aaaaaguuau auaucuuguc augauucuau uaauagcacc agcauauagu auuaggugca      660

uaggcguuag caacagagau uucguagaag ggaugucagg aggaacaugg guagauguug      720

uauuagagca ugggggaugu gucaccguaa uggcacagga uaaaccuaca guagacaucg      780

aauuaguuac aacaacagua ucaaauaugg cagaaguaag aucuuauugc uaugaagcau      840

caauaucaga cauggcuagu gauagccgau gcccuacaca aggagaagca uacuuagaua      900

aacaaucaga uacacaauau guauguaaaa gaacccuagu agauagagga uggggaaaug      960

gauguggauu auucggaaaa ggaucauugg uaacuugugc aaaauuugca uguucaaaga     1020

aaaugacugg aaaaagcaua cagccugaaa aucuugaaua cagaauaaug cuuaguguac     1080

acggaucuca acauucaggg augauaguaa acgauacagg gcaugaaaca gaugaaaaua     1140

gagcgaaagu agaaauuaca ccuaauaguc ccagagcuga agcaacacua ggaggauucg     1200

gaagccuagg acuugauugu gaaccccgua caggauuaga uuuuucagac uuauauuauu     1260

ugacaaugaa caauaaacau uggcuagucc acaaagaaug guuccacgac auaccgcuac     1320

ccuggcacgc aggagcggac acugguaccc cacauuggaa uaauaaagaa gcauuaguag     1380

aguucaaaga ugcacaugcu aaacgacaaa caguaguagu acuuggaagu caagaaggag     1440

caguacauac agcauuagcg ggugcacuag aagcagaaau ggauggagca aaaggaagau     1500

uaaguagugg gcaucuaaaa ugcagacuca aaauggauaa acuaagguua aaaggaguau     1560

cauauucauu auguacagca gcuuuuacau uuacuaaaau accugcagaa acguuacaug     1620

gaacgguaac ugucgaagua caauaugcug gaaccgacgg accaugcaaa guaccugcac     1680

aaauggcagu agauaugcaa acauuaacuc cuguaggcag auugauaacu gcaaacccug     1740

ucauaacgga aucaacagaa aauaguaaaa ugaugcuuga auuagaccca ccauuuggag     1800

auaguuauau aguaaucgga guaggggaaa agaaaauaac acaucacugg cacaggucgg     1860

ggucaacaau aggaaaagcg uuugaagcaa caguaagagg agcaaaaagg auggcaguau     1920

uaggugacac agcuugggau uuuggaagcg uagguggugc auuaaauagu uuaggaaaag     1980

gcauucauca aaucuucgga gcggcauuua aaagucuauu cggaggaaug ucuugguuuu     2040

cucaaauacu uauuggaacu uuauuaaugu ggcuaggauu aaauacuaaa aacgguagua     2100

uaaguuuaau gugccucgca uugggaggag uauuaauauu ucucaguaca gcgguaagcg     2160

cagacguagg guguagcgua gauuucucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6995
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 2 opt

<400>  6995
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauaaaugc ucggaaggaa aaaaaaagaa gaggagccga uacaagcgua ggaauaguag      120

gauuacuucu aacgacagcg auggcggcag aggucacaag gagaggaucc gcauauuaca      180

uguacuugga uagaaaugau gcaggagaag caauaucauu uccaacaaca cuaggaauga      240

acaaauguua uauacaaaua auggaucuag gacauaugug cgaugcuaca augucauaug      300

agugcccaau guuggaugaa ggaguagagc cagaugaugu agacuguugg uguaacacaa      360

caucaacuug gguuguauac gggacguguc aucauaaaaa aggggaggcg aggaggucga      420

gaagagcagu aacguuacca ucacauagca caagaaaauu acaaacacga agucaaaccu      480

ggcuagaaag uagagaauau acaaaacauu uaauuagagu agaaaauugg auauuuagaa      540

acccaggauu agcguuagcu gcagcagcaa uagcauggcu uuuagguagc ucgacaaguc      600

aaaaaguuau auaucucguu augauacucu uaauagcacc ugcauauagu auaagaugua      660

uuggaguaag uaaucgagac uuuguagaag guaugucagg aggaacaugg gucgaugugg      720

uacuggaaca uggaggaugu guaacuguua uggcacaaga uaaaccaaca guagacauag      780

aguuaguaac aacaaccgua ucaaacaugg cagaagugcg uaguuauugc uaugaagcgu      840

cgauaucaga cauggcaagu gauagccgau guccaacuca aggagaggca uauuuagaua      900

aacaaaguga uacucaauau guaugcaaaa gaacacuagu cgauagaggu ugggguaacg      960

gaugcggauu auucgggaaa ggcucauuag uaacuugugc uaaauuugcu uguucaaaaa     1020

aaaugacugg aaaaagcauu caaccagaga auuuggaaua ucguauaaug uuaaguguuc     1080

auggaucaca acacucaggu augauaguca augauacagg acaugaaaca gacgaaaaua     1140

gggcaaaagu agaaauaaca ccaaauaguc cuagagcaga agcaacauua ggcggauuug     1200

ggaguuuagg cuuagacugu gagccaagaa cuggauuaga cuuuagcgac uuauauuacu     1260

uaacaaugaa caacaaacau ugguuaguac acaaagagug guuucaugau auaccguuac     1320

cauggcaugc aggagcagac acuggaacac cacauuggaa uaauaaagag gcauugguag     1380

aauuuaaaga ugcccaugcg aaacgacaaa cagugguagu acuaggauca caagaaggcg     1440

caguacauac agcacuugcu ggcgccuugg aagcagaaau ggacggggcu aaagguagau     1500

uaaguucagg ucauuuaaaa uguagauuaa aaauggacaa auuaagauua aaaggugugu     1560

cauauucauu gugcacagca gcauucacau uuacuaaaau accagcugaa acauuacacg     1620

guacaguaac aguagaaguu caauaugcag guacggacgg cccauguaaa gucccagcuc     1680

aaauggcggu agauaugcag acauuaacuc caguugguag acuaauaaca gcaaauccug     1740

uaaucaccga aaguacugaa aauucaaaaa ugauguugga acuugaucca ccauuuggag     1800

auucauauau uguaauaggu guaggggaaa aaaaaauaac acaucauugg cacagaagug     1860

gaaguacgau uggaaaagcc uuugaagcaa cgguaagggg ugcaaaacga auggcaguau     1920

uaggugauac agcaugggau uuuggcagug uaggaggugc acuaaauuca uuaggaaaag     1980

gaauacauca aauauucgga gcagcauuua aaagcuuguu uggugguaug ucaugguuuu     2040

cacaaauacu aauaggaaca cuauuaaugu gguuaggauu aaauacaaaa aaugggagua     2100

uaagucucau gugucuggca uuaggcggag uacucauauu uuuaaguaca gcaguuagcg     2160

cagauguagg auguagugua gauuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6996
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 2 opt

<400>  6996
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaaugc acgaaaagaa aagaaaagga gaggagcuga cacuuccgua ggcauuguag      120

gauuacucuu aacaacugcu auggcagcug aagucaccag aagaggguca gcauauuaca      180

uguaucuuga uagaaaugac gcaggggaag caauuagcuu uccaacaaca cuuggaauga      240

auaaauguua cauacaaaua auggaccucg gacauaugug cgacgcaacu augagcuaug      300

aauguccaau gcuagacgaa ggaguagaac cagaugacgu agacuguugg ugcaauacua      360

caaguacuug gguaguuuau gggacaugcc aucauaagaa aggugaagca agaagaucua      420

ggagagcagu uacauuaccu agucauagca cgagaaaacu acaaacaaga ucccaaacau      480

gguuagaauc aagggaauac acaaaacauc uuauaagagu agaaaauugg auauucagaa      540

aucccgguuu cgcucuagca gcagcugcua uagcuuggcu auugggaagu agcacuagcc      600

aaaaaguaau auacuuagua augauacuau uaauagcucc ugcauauucu auaagaugua      660

uuggaguaag uaacagagac uucguugaag gaaugagugg aggcacaugg guagaugucg      720

uacuugaaca cggaggaugu guaacaguaa uggcacaaga uaaaccgaca guagauauug      780

aauugguaac aacaacaguc aguaauaugg cagaaguuag aaguuauugu uaugaagcua      840

guauaaguga cauggcauca gauucacgau guccuacaca aggagaggcu uaucuagaca      900

aacagucuga uacacaauac guguguaaaa gaacuuuagu ugaucgagga uggggaaaug      960

gauguggacu auuuggaaaa ggaucauuag uaacaugugc aaaauucgca uguucaaaga     1020

aaaugacagg aaaaagcaua caaccagaaa auuuagaaua cagaauaaug uuaagugugc     1080

auggauccca acacagcggc augauaguaa augauacagg acacgaaaca gaugaaaaca     1140

gagcaaaagu ugaaauuaca ccaaauaguc cacgagcgga agccacacuu ggaggguucg     1200

gaucacuagg acuugacugu gaaccaagaa cuggauuaga uuucucagau uuauauuacu     1260

uaacaaugaa uaacaaacau ugguugguac auaaagaaug guuucacgau auuccgcuac     1320

cauggcaugc uggagcagau acagggacac cacauuggaa uaacaaagaa gcucuaguag     1380

aauuuaaaga ugcacaugca aagagacaaa caguaguagu acuaggaagu caggaaggag     1440

caguacauac agcacuagca ggagcucuag aagcugaaau ggauggugca aaaggaagac     1500

uaaguagcgg acaucuaaaa uguagacuaa aaauggacaa acuaagacua aaggggguuu     1560

cauauucauu auguacagca gcuuuuacau ucacaaaaau accugcagaa acuuuacaug     1620

guacaguaac aguugaagua caauaugcug gaacagacgg acccuguaaa guaccagcac     1680

aaauggcagu agauaugcaa acauuaacac caguuggacg auuaauaaca gcaaaucccg     1740

uuauaaccga aaguacagaa aauucuaaaa ugauguuaga auuagauccc ccauucggag     1800

acagcuauau agugauugga guaggagaaa agaaaauuac acaucacugg uaccgcucag     1860

gaucaacaau aggaaaagcc uucgaagcua cuguaagagg ugcaaaacga auggcaguac     1920

uaggggacac agcgugggau uucgguucag uaggaggagc acuuaacucc uuagguaaag     1980

gcauacauca aauauuugga gcagcuuuua aaucucuauu ugggggcaug ucaugguucu     2040

cacaaauuuu aaucggcaca cuacuaaugu gguuaggacu aaauacuaaa aacggaucaa     2100

uaagucucau gugucuagcc cuaggaggag uacuaauauu ucuaaguacu gcuguaucag     2160

ccgauguggg augcagugua gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6997
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 2 opt

<400>  6997
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauuaaugc acgaaaagaa aaaaaaagaa gaggagcuga uacaagugua ggcauaguag      120

guuuacuguu aacuaccgcu auggcagcag aaguaacgag aagaggauca gcauauuaua      180

uguauuuaga uagaaaugau gcuggggaag caauaucauu uccuacaaca uuaggaauga      240

auaaaugcua uauucaaaua auggaucugg gacacaugug ugaugcaaca augucauaug      300

aaugccccau guuagaugaa gguguagaac cagaugaugu agacuguugg uguaauacaa      360

caucaacuug gguaguauau ggaacaugcc aucauaaaaa aggagaagcg agaagaagca      420

gaagagcugu aacauuacca ucacauagua cuagaaaacu acaaacaaga agccaaacau      480

ggcuugaaag uagggaauau acuaaacauc uuauaagagu agaaaauugg auauuuagaa      540

auccaggauu cgcauuagca gcugcagcua uagcauggcu auuaggauca uccacaaguc      600

aaaaaguaau auauuuggua augauauuac uaauugcacc agcauacuca auaagaugua      660

uaggagucag uaauagagau uuuguggaag gaaugagcgg aggaacaugg guagacguag      720

uuuuagaaca cgguggaugc guuacaguua uggcacaaga uaaaccaacu guagacauag      780

aauuaguuac aaccacagua aguaauaugg cagagauuag aucauauugu uaugaagcau      840

ccauaagcga uauggcaucg gauaguagau guccaacaca gggcgaagca uaccuagaua      900

aacaaagcga uacgcaauau guuuguaaaa gaacgcuagu agauagagga ugggguaaug      960

guuguggacu uuucggaaaa ggaagccugg uaaccugugc aaaauucgca uguucuaaaa     1020

aaaugacagg aaaaucuauu caaccagaaa aucuggaaua uagaauaaug uuaagcgucc     1080

auggaaguca acauuccgga augauuguaa augauacggg acaugaaaca gacgaaaaca     1140

gagcaaaagu ugaaauaaca ccaaauuccc caagagcaga agcaacccua ggaggauuug     1200

gaucauuagg auuagauugc gaaccuagaa caggacuaga cuucucagac cuguauuacc     1260

uaacaaugaa caauaaacau ugguuaguac auaaagaaug guuccaugau auaccacuac     1320

cguggcaugc aggagcugac acuggaacac cccauuggaa uaacaaagaa gccuuaguag     1380

aguuuaaaga ugcacacgca aaacgacaaa caguagucgu ccuagguagu caagaaggug     1440

cuguacauac agcauuagcu ggugcucuag aagcagaaau ggauggagcu aaaggaagac     1500

uaucaagugg acaccuaaaa ugccgauuaa aaauggauaa auuaagacua aaaggaguaa     1560

guuacaguuu augcacagcg gcauuuacau uuacaaaaau acccgcagaa accuuacaug     1620

guacaguaac cguagaagua caauaugcag gcacggaugg accauguaaa guaccagcac     1680

aaauggcugu ugauaugcaa acacuaacac cgguaggccg auuaauaacu gcuaacccag     1740

uaaucacaga aaguacugaa aauaguaaga ugauguuaga auuggaccca ccauuuggag     1800

acucauauau aguaaucggc guaggagaaa aaaaaauuac acaucacugg cauagaucag     1860

gaaguacaau aggaaaagcc uucgaagcaa cuguaagagg agcaaagaga auggcaguac     1920

uaggagauac agcaugggau uuuggaucgg uuggaggagc ucucaauagc uugggaaaag     1980

gcauacauca aauauuugga gcagccuuua aaaguuuguu cggaggaaug aguugguuuu     2040

cucaaauauu aaucgggaca cuacuaaugu ggcugggauu aaauacaaaa aauggcucaa     2100

uaagucucau gugcuuagcu uuagggggcg uuuuaauauu uuuaaguaca gcaguaucag     2160

cagacguugg uugcagcgua gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6998
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 2 opt

<400>  6998
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc ccgaaaagaa aaaaaacgaa gaggagccga cacaagugua ggaaucguag      120

gguuauuacu uacgacggcu auggcggcag aaguaacacg aagaggaagu gccuacuaca      180

uguacuugga cagaaaugac gcaggagaag caauaucauu uccaacaaca cuagguauga      240

acaaauguua uauucaaauc auggaucuag gucauaugug ugaugccacu augagcuacg      300

aaugcccaau gcuugaugag ggcguagaac cugaugaugu ggacuguugg ugcaacacaa      360

cuaguacuug ggucguguac ggcacauguc aucauaaaaa aggagaagca agaagaagca      420

ggagagcagu aacguuaccg agccauucaa caagaaaacu acaaacaaga agccaaacau      480

gguuagagag ucgagaauau acaaaacacu ugauaagagu agaaaacugg auauuuagaa      540

auccagguuu cgcauuagca gcagcagcaa uugcauggcu auuggguagu uccacaaguc      600

aaaaaguaau uuauuuaguu augauauuau uaauagcucc cgcauacuca auaagaugca      660

uaggaguaag caauagagau uuuguagagg gaauguccgg aggaacgugg guagauguag      720

ucuuagaaca ugguggaugu guaacuguaa uggcacaaga uaaaccaacu guagauaucg      780

aacuuguaac cacaaccgua ucaaauaugg cagaaguuag aucguacugc uaugaggccu      840

caauaucaga uauggcauca gauucaagau guccaacaca aggugaagca uaucuugaca      900

aacaaucuga uacucaauau guauguaaaa ggacacuagu agauagaggu ugggguaaug      960

gcuguggacu auuugguaaa ggaucucuag uaacaugugc aaaauuugca uguucaaaaa     1020

aaaugacagg aaaaaguaua caaccagaaa aucuagaaua cagaauaaug uuauccguac     1080

augggagcca acauagcgga augauaguaa augauacugg acacgaaaca gaugaaaauc     1140

gagcaaaagu agaaauaacu cccaauucac cuagagcuga agcaacccua ggagguuuug     1200

gaaguuuagg auuagauugu gaaccacgaa caggucuaga uuuuucagau cuauauuauu     1260

uaacaaugaa uaacaaacac ugguuaguac auaaagaaug guuucaugau auaccauuac     1320

cauggcacgc aggggcagac acaggaacuc cccacuggaa caacaaagaa gcuuuagugg     1380

aauuuaaaga ugcacaugca aaaagacaaa caguaguagu acuaggcucu caagaaggag     1440

cugugcauac ugcauuagca ggagccuuag aagcagaaau ggauggagca aaagggagau     1500

ugucaucagg gcauuuaaaa ugucgacuaa aaauggauaa auuaagauua aaagggguca     1560

gcuauucacu auguacggca gcuuuuacau uuacaaaaau accagcagaa acacuccaug     1620

guacaguaac aguagaaguu caauaugcag guacagaugg accauguaag guucuagcac     1680

aaauggcagu agauaugcaa acguuaacuc cuguaggaag auuaauaaca gccaauccug     1740

uaauaacaga aucaaccgaa aauucaaaaa ugauguuaga auuagauccu ccuuuuggag     1800

auucuuauau aguaauaggu guuggugaaa aaaaaauaac acaucauugg cauagaagug     1860

gaagcacaau agguaaagca uuugaagcaa caguaagagg agcaaaacga auggcuguac     1920

uaggagacac agcuugggau uucggaagug uaggcggagc auuaaauuca uuaggaaagg     1980

guauacauca aaucuuuggg gcugcauuca aaaguuuauu ugguggaaug agcugguucu     2040

cacaaauucu uaucggaaca cuauuaaugu ggcuaggucu uaauacaaaa aaugggucaa     2100

uaucuuuaau guguuuagca uuagggggag uacuuauauu ccucucaaca gccguaucag     2160

cagacguugg auguucagua gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  6999
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 2 opt

<400>  6999
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc cagaaaagaa aaaaaaagac guggugcuga caccagugua ggaauaguag      120

gauuauuguu aacaacugca auggcggcag aaguaacaag aaggggaagu gcauauuaua      180

uguaucuaga uagaaacgac gcaggcgaag caauaaguuu cccaacaaca uuaggaauga      240

acaaauguua uauacaaaua auggaccucg ggcacaugug cgaugcaaca augaguuaug      300

aaugcccaac acuagacgaa ggaguugaac cggaugacgu agauuguugg ugcaacacaa      360

caaguacgug gguuguauau gguacguguc aucauaaaaa aggugaagcc aggaggucaa      420

gaagggcagu aacauugcca ucccauagua cacggaaauu gcaaacaaga ucacagacuu      480

ggcuugaaag cagagaauau acaaaacauu uaauaagagu agaaaauugg auauuucgga      540

auccaggauu ugcauuagca gcugcugcaa uugcauggcu ucuagguucg ucaacaucac      600

aaaaaguaau auauuuggua augauauugu uaauugcucc agcauauuca auucguugua      660

uagguguaag caaucgugau uucguagaag gaaugagcgg agggacgugg guugaugucg      720

uguuagaaca uggagggugu guaacaguaa uggcacaaga uaaaccaaca guagauauag      780

aauuaguaac aacuacagua agcaacaugg cagaaguaag aucauauugu uaugaagcaa      840

guauauccga cauggccucc gauaguagau gucccacaca aggugaagcg uauuuagaua      900

agcaaucuga uacacaauac guaugcaaaa gaacacuagu agauagaggu uggggaaaug      960

gcugcggauu auuuggaaaa ggcagucuag uuacaugcgc aaaauuugca uguagcaaaa     1020

aaaugacagg aaaaaguauu caaccagaaa acuuagagua uaggauaaug uuaucgguac     1080

acggaaguca acacucagga augauuguaa acgacacggg ccacgaaacu gacgaaaaua     1140

gagcaaaagu agaaaucacg ccaaauucac cacgagccga agccacauua ggaggauuug     1200

gaucacuggg auuagauugc gaaccuagaa cgggauugga uuuuagugau uuauauuauu     1260

uaacuaugaa uaauaaacau ugguuaguac auaaagagug guuucaugau auaccucuac     1320

cuuggcaugc cggggcagau acuggaacuc cacauuggaa uaauaaagaa gcauuaguag     1380

aauuuaaaga ugcccacgca aaaagacaaa caguaguugu auuaggaagu caggaaggag     1440

cuguacauac agccuuagca ggagcacuag aagcagaaau ggauggagca aaaggaagau     1500

uaucaagugg acacuuaaaa uguagauuaa aaauggacaa acuaagacuu aaaggugugu     1560

cauacuccuu auguacagcc gcuuuuacuu uuacuaaaau uccagcggag acuuuacaug     1620

ggacuguaac aguagaaguu caguaugcag guacagaugg uccaugcaaa guaccagccc     1680

aaauggcagu ugauaugcaa acauuaacac caguaggaag auuaaucaca gccaaucccg     1740

uaaucacaga auccacagaa aauaguaaga ugaugcuaga auuagaccca ccguuuggag     1800

auaguuauau aguaauagga guuggggaaa agaaaauaac acaucauugg caccgaagcg     1860

guagcaccau aggaaaagcu uuugaagcaa caguuagagg agcuaaaaga auggcaguau     1920

ugggugauac agcaugggau uucggaucag uagguggagc auuaaacagc uuggguaaag     1980

gaauacauca aauuuucgga gcagcauuua aaagcuuauu uggaggaaug aguugguucu     2040

cacaaauauu aauaggaaca cuauuaaugu gguuaggacu aaauacaaaa aauggaagua     2100

uaaguuuaau guguuuagca uuagggggag uacuaauauu cuuaucaaca gcaguaucug     2160

cugacguagg augcucagua gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7000
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 2 opt

<400>  7000
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uaauaaaugc cagaaaagaa aagaaaagga gaggagcaga cacaagcgua ggaauaguag      120

gcuuauuauu aacaacagca auggccgcag agguaacucg aagaggauca gcguacuaua      180

uguauuuaga uagaaaugau gcaggagaag caauaucauu uccaacaaca uuaggaauga      240

acaaaugcua cauacaaaua auggauuuag ggcauaugug ugaugccacu augaguuaug      300

aaugcccaau gcuagacgag gguguggaac cagaugacgu agauuguugg uguaacacaa      360

caucuacaug gguuguauau ggaacuuguc aucacaagaa aggagaagca cgaagaagca      420

ggagggcagu aacacuacca agccauucaa cuagaaaacu ccaaacaaga agucaaaccu      480

gguuagaauc aagagaauau acgaaacauc uaauaagagu agaaaacugg auauuuagaa      540

aucccggauu ugcauuagca gcagcagcaa ucgccuggcu auuaggaagu ucaacaaguc      600

aaaaagugau auaucuaguc augauacuac uaauugcacc agcuuauagc auaagaugua      660

ucggugucag caauagagac uucguugaag guaugucagg uggaacgugg guagacguug      720

uuuuagaaca cggcggaugu guaacaguaa uggcacaaga uaaaccaacg guagacauag      780

aacuugugac aacuacuguu aguaacaugg cagaaguacg aagcuauugc uaugaagcau      840

cuauaucaga uauggcauca gauucacgau guccuacaca aggagaagca uacuuagaua      900

aacaaagcga uacacaauau guuuguaaaa ggacauuagu agauagaggc uggggaaaug      960

guuguggauu auuuggaaaa gguucauugg uaacgugugc aaaauucgcu uguagcaaga     1020

aaaugacagg uaaaucaaua caaccugaaa acuuggaaua cagaauuaug cuaucaguac     1080

auggaucaca acauuccgga augauaguaa augauacugg ucaugaaaca gaugaaaacc     1140

gagccaaagu agaaauaaca ccuaauucuc caagagcaga agcuacauua ggaggauucg     1200

gaucacuugg ucuagauugc gaaccaagaa cuggacuuga cuuuagugau uuauacuauu     1260

uaacaaugaa uaauaaacau uggcuaguac auaaagaaug guuucaugac auaccauuac     1320

cauggcacgc aggagcagau accggaacac cacacuggaa caauaaagaa gcucuaguag     1380

aguucaaaga ugcucaugca aaaagacaga ccgugguagu auuaggauca caagaaggag     1440

cuguacauac ggcucuagca ggagcacuug aagcggaaau ggauggugca aaaggacgau     1500

uaaguucagg gcaucuaaaa uguagauuaa aaauggacaa auuaagauua aaaggaguaa     1560

guuauaguuu augcacagcc gcuuuuacau uuacuaaaau accagcagaa acguuacaug     1620

gaaccguuac cguagaagua caauaugcag gaacagaugg uccaugcaag guaccagcac     1680

aaauggcagu agauaugcaa acgcuaacac caguaggaag guuaaucacc gcuaauccag     1740

ugauaacaga aucgaccgaa aauuccaaaa ugauguuaga auuggaucca ccuuuuggag     1800

acuccuauau aguaauaggg guuggggaaa agaaaauaac ccaccauugg cauagaucag     1860

ggucaacaau aggaaaggcu uucgaggcaa ccgugcgagg agcaaaaaga auggcgguuu     1920

uaggggauac ugcaugggau uuuggaagug ugggaggagc auuaaacagc cuggguaaag     1980

guauacauca aauauucgga gcagcauuca aaucauuauu uggaggaaug agcugguuua     2040

gucagauacu aauuggaaca uuacugaugu ggcuuggauu aaacaccaaa aacggaucaa     2100

uuagcuuaau gugcuuagca cuugguggag uauuaauauu ccuaaguaca guaguuucag     2160

cagauguagg uugcucagua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7001
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 2 opt

<400>  7001
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc cagaaaagaa aaaaaaagaa gaggcgccga uacaucagua gggauaguag      120

gauugcuauu aacaacagcc auggcagcag aaguuacaag acgaggauca gcauauuaca      180

uguacuuaga uagaaaugau gcaggagaag cuauaaguuu cccgacuacu uuaggaauga      240

acaaguguua uauucaaauu auggacuuag gacacaugug ugacgcaaca augucauaug      300

aaugcccaau guuagacgaa ggaguagaac cagaugaugu agauuguugg uguaacacua      360

caagcgcuug ggucgucuac gggacaugcc accacaaaaa aggagaagcu agacgaagua      420

gaagagcagu aacccuacca ucucauagua cacgaaaacu acaaacuaga ccucaaaccu      480

gguuagaauc aagagaauac acaaaacauu uaauacgagu agaaaauugg auauucagaa      540

acccaggauu cgcguuggca gcggcggcaa uugcaugguu auuaggauca aguacaucuc      600

aaaaagucau auauuugguu augauacuuu uaauagcucc agcauauagc auacgaugua      660

uaggaguaag caauagagac uuuguggaag gaaugucagg agggacaugg guugacguag      720

ucuuagaaca uggaggaugu guaacgguca uggcacaaga uaaaccaaca guugauauag      780

aauuaguaac aacaacagua ucaaauaugg cagaaguuag aucauauugu uaugaagcau      840

caaucucaga cauggcaagu gacucaagau gucccacgca aggugaagca uaucuagaca      900

aacaaaguga uacacaauau guaugcaaaa gaacacuugu agacagagga uggggcaacg      960

gaugcggucu cuuugguaag ggcagcuuag uuacaugugc aaaauucgcg uguaguaaaa     1020

aaaugacagg uaaaucaaua cagccugaaa aucuugaaua ucgaauuaug uuaucuguuc     1080

acggaucaca acauucuggc augauuguaa augacacagg ucacgaaaca gaugaaaaca     1140

gagcuaaagu agaaauaaca ccgaacucac caagagcaga agcuacuuua ggagguuuug     1200

ggaguuuagg acuagauugc gaaccaagaa caggacuuga uuuuagugau uuauacuauu     1260

uaaccaugaa uaauaaacau uggcugguac auaaagaaug guuucaugau auuccauuac     1320

cauggcaugc aggagcugau acaggaacac cccacuggaa uaauaaagaa gcgcuuguag     1380

aauuuaaaga cgcacaugcc aaaagacaaa caguaguagu uuuagguagu caagaagggg     1440

caguccauac agcacuagcg ggagcauuag aagcagaaau ggauggagca aaaggacgau     1500

uaagcucagg acaucuaaaa uguagacuga aaauggauaa auuaaggcua aaaggaguaa     1560

gcuauagucu uugcacagca gcauucacuu uuacaaaaau uccagcggaa acacuacaug     1620

gaacuguaac aguagaagug caauacgcag gaacugaugg accguguaaa guuccagcac     1680

aaauggcagu agauaugcaa acauuaacac caguaggacg uuuaauaaca gccaauccag     1740

uaauaacaga aucaaccgaa aacucgaaaa ugaugcuaga auuagauccu ccuuuuggag     1800

auucguacau aguuauaggu gucggagaaa aaaagaucac acaucacugg cauagaagcg     1860

gcaguacaau aggaaaagca uuugaagcua ccguacgugg agcuaaaaga auggcaguuc     1920

uaggcgauac ugcaugggau uuuggcucag uuggaggugc auuaaacagc uuagggaaag     1980

gaauacauca aauuuuuggu gcagcauuca aaagcuuguu uggaggaaug ucaugguuua     2040

gccaaauauu aauuggaacu uuacuuaugu gguuaggacu aaauacaaaa aaugguagua     2100

uaucauuaau gugucuugca uuagguggag uauugauuuu uuuaucaaca gcaguaagcg     2160

cagauguagg auguucagua gacuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7002
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 2 opt

<400>  7002
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc acgaaaagag aaaaagagac gaggcgcaga cacaagugua ggaauaguag      120

gccuacuuuu aacuacagca auggcugcag aaguaacgag acgagguucc gcauauuaua      180

uguaccuuga caggaaugac gcaggggaag caauauccuu uccaaccaca uuaggaauga      240

auaaauguua cauacaaaua auggaucuag gacauaugug ugaugcaaca augaguuaug      300

aauguccaau gcuggaugaa ggaguagaac ccgaugacgu agauuguugg ugcaauacua      360

caucaacaug gguaguauau gggaccuguc aucauaaaaa aggagaagca agaaggagua      420

gaagagcagu cacauugccg agucacucaa caagaaaauu acaaacaaga ucccaaacau      480

ggcuugaauc uagagaauac acaaaacauc uuauaagagu ugaaaauugg auuuucagaa      540

accccggauu cgcgcuagca gcagcagcaa uagcauggcu ccuuggauca uccacaagua      600

aaaaaguaau auacuuagug augauauuac uaaucgcacc agcauacagu auacguugca      660

uaggaguauc aaaucgagau uucguugaag ggaugucugg agguacaugg guagauguug      720

uacuagaaca ugguggaugu guuacaguaa uggcgcaaga caaaccuaca gucgauauag      780

aacuaguaac aacuacagua agcaauaugg cagaaguccg aucauacugu uacgaagcaa      840

guauaucuga cauggcaagc gacagucgau guccaacaca aggagaagca uaucuagaua      900

aacaaucuga cacacaauau guuugcaaaa gaaccuuagu ugauagagga ugggguaaug      960

gguguggcuu auuuggcaag gguagucuug uaaccugugc aaaauucgca uguaguaaaa     1020

aaaugacagg aaaaagcaua caaccagaaa aucuagaaua uagaauaaug uuaucaguac     1080

augguaguca acauucagga augauaguua augacaccgg acacgaaacu gaugaaaauc     1140

gagcaaaagu agaaauaaca ccaaauaguc cacgagcaga agcuacauua ggcggauuug     1200

gaucguuagg auuagauugu gaaccaagaa cuggauuaga uuuuagugau cuauauuacc     1260

uaacaaugaa uaauaaacac ugguuaguac auaaagaaug guuucacgac auaccacuac     1320

ccuggcacgc uggagccgau acuggaacac cacauuggaa caacaaagaa gcacuaguag     1380

aguuuaaaga ugcacacgca aaaagacaaa caguaguagu auuaggauca caagaaggcg     1440

caguccauac agcacuagca ggagcauuag aagcggagau ggacggagca aaaggaagac     1500

uaaguucagg ccauuuaaaa ugcaggcuaa aaauggauaa auuaagauua aaaggaguau     1560

cauauucacu auguaccgcc gcauuuacau uuacaaaaau accagcagaa acuuuacaug     1620

gcaccguuac ugucgaaguc caauaugcug gaacagaugg accauguaag guuccagcuc     1680

aaauggcagu ggauaugcaa acacuaacuc caguagguag acuaauaaca gcaaauccgg     1740

uaauuacuga aucaacagaa aacucaaaaa ugaugcuaga auuagauccu ccguucggag     1800

acucguacau aguuauaggu guaggugaaa aaaaaauaac acaucauugg cauagaucag     1860

gaucaaccau agggaaagcu uuugaggcua cgguaagagg agcaaaacga auggccguuu     1920

uaggagauac ugcaugggau uuuggaagcg uaggaggagc auuaaacuca uuaggaaaag     1980

gaauacauca aauauuugga gcagcauuua aaagucuauu uggaggaaug ucaugguuuu     2040

cacaaauacu aauaggaacu cuguuaaugu gguuaggacu aaauacaaaa aauggaucua     2100

uaagccuuau gugccuagcu cuaggaggag uuuuaauauu cuuaaguaca gcuguaucag     2160

cagauguugg auguucugua gacuucucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7003
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 2 opt

<400>  7003
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc acgaaaagaa aagaaaagaa gaggcgcuga uacaagcgug ggaauugugg      120

gauuauuauu aacaacagca auggcagcgg aaguuacaag aagaggaagu gccuauuaua      180

uguaucuaga uagaaaugac gcaggagagg caaucucauu uccaacuaca cuuggaauga      240

auaaauguua cauccaaaua auggacuuag gacauaugug cgaugcaacu augucauacg      300

aauguccgau guuagaugaa ggaguagagc cagacgaugu agauugcugg uguaauacaa      360

cuucaacaug gguaguauac ggcacguguc aucauaagaa aggcgaagcu agacgaucca      420

ggagagcagu aacauuaccu ucacauagua caagaaaauu acagacaaga ucacaaacau      480

gguuagaauc uagagaauau acaaaacauc uaauuagagu ugaaaacugg auauuuagaa      540

acccaggauu cgcacuagca gcugcagcaa uagcaugguu acuaggcagc ucuacgagac      600

aaaaaguaau auaccuuguu augauacucc uaauagcacc agccuauuca auuagaugca      660

uaggaguaag uaacagagau uuuguugaag gaaugagcgg agguacaugg guagauguag      720

uauuagaaca uggaggaugu gugacaguaa uggcacagga uaaaccaacu guagacauag      780

aauuaguaac aacaacugua agcaauaugg cagaaguaag aaguuacugu uaugaagccu      840

caaucagcga uauggcaagu gauucaagau guccaacaca aggugaggcu uauuuagaua      900

aacaaagcga cacacaauau guaugcaaaa ggacauuagu agacagagga uggggaaacg      960

guugcggccu auucggaaaa ggaagucuag uuacaugugc aaaauuugcu uguucuaaga     1020

aaaugacagg aaaaagcaua cagcccgaaa accuagaaua ucgcauaaug uuaagcguac     1080

auggaucaca acauagugga augauaguua augauacagg acacgaaaca gaugaaaaca     1140

gagcaaaagu ggaaauaacu ccaaauucac ccagagcaga agcaacacua ggaggauucg     1200

gaucacuagg acuagauugu gaaccgagga ccggauugga uuuuagugau uuguacuauu     1260

ugacuaugaa uaacaaacau uggcuaguac acaaagaaug guuucaugau auaccauuac     1320

cauggcaugc aggagcagau acggguacac cacauuggaa uaacaaagaa gcauuaguag     1380

aauuuaaaga cgcacaugca aaacgacaaa caguaguagu acuugggagu caggaaggag     1440

caguacauac agcauuagca ggagcauuag aagcagaaau ggauggugca aaagguagac     1500

uuucauccgg acaccugaaa ugucgacuaa aaauggauaa auuaagauua aaagggguga     1560

gcuacagcuu guguacugca gcauuuacuu uuacuaaaau accagcugaa acacuucaug     1620

gaacaguaac uguagaagug caguaugcag gaacagaugg uccauguaaa guaccagcac     1680

agauggcugu agauaugcaa acucuaacac cuguuggcag gcuaauaacu gcaaauccag     1740

uaauaacaga aucaacagaa aacucaaaaa ugauguugga auuagaucca ccauuuggag     1800

auaguuacau aguaauaggu guuggggaaa agaaaauuac ucaucauugg cacagaucag     1860

gaucuacgau aggaaaagca uuugaagcua cuguaagagg cgcaaaaagg auggcaguau     1920

uaggugauac ggcaugggac uuuggaucug uagguggagc guugaauagu cuagguaaag     1980

gaauacauca aauauuugga gcagcauuua aaucauuauu ugguggcaug aguugguuua     2040

gucagauacu uauaggaacu cuauuaaugu gguuaggauu aaacaccaaa aauggaucaa     2100

uaucacuuau guguuuagca uuagggggag uccuaaucuu ccuaucaaca gcaguaagcg     2160

cggauguagg auguucagua gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7004
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 2 opt

<400>  7004
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgua       60

uuauaaaugc aagaaaagaa aagaaaagaa gaucagcaga uacaucaguu gguauugugg      120

gcuuacuucu cacaacagcg auggcagccg aaguaacuag aagaggauca gcuuacuaca      180

uguauuuaga caggaaugac gcuggggaag cuauaucauu uccuacaaca uuaggaauga      240

auaaaugcua uauacaaaua auggaccuug gacacaugug ugaugcaacc augagcuaug      300

aauguccaau guuagacgaa ggaguagagc cagaugaugu agacuguugg ugcaauacua      360

cuuccaccug ggugguauau ggaacaugcc accauaagaa aggugaagca agaagaagua      420

gacgagcagu aacacugcca ucacacagua caagaaaacu ucaaacaaga agccaaacau      480

ggcuagaaag uagagaauau acaaaacauu uaauaagagu agaaaacugg auauuuagaa      540

auccuggauu ugcuuuagcu gcugcagcaa uagcauggcu auugggaagc aguacaagcc      600

aaaaagucau auaccuagua augauacuuc uuauagcacc agcauauuca auaagaugua      660

uaggaguauc aaauagagau uuugucgaag gcaugagugg aggaacaugg guagauguug      720

uuuuagaaca uggaggaugu guaacaguaa uggcacaaga uaaaccaaca guagacauag      780

aauuaguaac aacaacaguc ucaaacaugg cagaaguaag aucauauugu uacgaagcau      840

caauuucaga uauggcuuca gauucaagau guccuacaca aggagaagcc uaccuagaua      900

aacaaaguga cacucaauac guaugcaaaa gaacucuagu agauagaggu uggggaaacg      960

gauguggguu auuuggaaaa ggaagccucg uaacaugugc aaaauucgcc ugcaguaaga     1020

aaaugacagg aaaaagcaua caaccagaaa acuuagaaua uagaauaaug cuuaguguac     1080

acgguucuca acauucagga augauaguaa acgauacagg ccaugaaaca gaugaaaaca     1140

gagcgaaagu agaaauaaca ccaaauucac ccagagcaga agccacacuu ggaggauuug     1200

gcucauuagg auuagauugu gaaccacgaa caggacuaga cuuuagugau cuauauuacu     1260

uaaccaugaa uaacaaacac uggcuaguac auaaagagug guuucacgau auuccacucc     1320

cauggcacgc aggagcagau acuggaacac cgcauuggaa uaauaaagaa gcccucguag     1380

aauuuaaaga ugcacaugcu aaaaggcaaa cggugguagu auuaggaagu caagaaggag     1440

ccguacacac agcauuggca ggggcacuag aggcugaaau ggacggagca aaaggucgau     1500

uaucaagugg acauuuaaaa uguagauuaa aaauggauaa auuaaggcua aaaggaguau     1560

cguauucauu augcacagca gcauuuacau uuacaaaaau uccggcggaa acacuacaug     1620

gaacaguaac aguagaagua caauaugcgg gaacagacgg uccauguaaa guaccugcac     1680

aaauggcagu agauacacaa acuuugacac caguaggaag acuaauuaca gcaaauccag     1740

uaauaacuga aucaacagaa aauucaaaaa ugauguuaga auuagaucca ccuuucggag     1800

auaguuacau aguaauugga gugggggaaa agaaaauaac ccaccacugg caccgaagcg     1860

guucaacuau aggaaaagca uuugaggcaa caguuagagg cgcaaaaaga auggcgguau     1920

uaggagacac agcaugggau uucggaucug uaggaggggc cuuaaacuca cuaggaaaag     1980

gaauacauca aauauuugga gcagcguuua aaucacuauu uggugggaug ucuugguuua     2040

gccaaauacu uauaggcacg cuacuaaugu ggcucgguuu aaauaccaaa aauggaucua     2100

uaaguuuaau gugucuagca uuaggaggag uacuaauauu uuugaguaca gcagugucag     2160

cugauguagg cuguucagua gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7005
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 2 opt

<400>  7005
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc aagaaaagaa aaaaaaagac gaggagcaga cacuaaugua gguaucguag      120

gacuauuauu aacgacagcc auggcagcug aaguaacccg aaggggaucg gccuauuaca      180

uguauuuaga uagaaaugau gcaggagaag caauaaguuu uccaacaacg uuagguauga      240

auaaauguua cauacaaaua auggauuuag gucauaugug ugacgcaaca augaguuacg      300

aaugcccaau guuagaugaa gguguggaac ccgaugacgu ugauugcugg ugcaauacaa      360

ccucaacaug gguugucuau gggacauguc aucauaaaaa aggagaagca agaagaagua      420

gaagggccgu uacucuucca agccauagca caagaaaacu ccaaacuaga ucacaaacau      480

ggcuagaaag uagagaauac accaaacacu uaauaagagu agaaaauugg auauuuagaa      540

auccaggauu ugcauuagca gcagcagcua uagcauggcu auuaggaucg ucuacaaguc      600

aaaaaguaau auauuuaguu augauacuac uaauagcacc agcauacagc auaaggugca      660

uaggcguauc aaacagagau uuuguugaag gaaugucagg aggaaccugg guagauguag      720

uguuagaaca uggagggugc gugacaguaa uggcccaaga uaaaccaaca guugauauag      780

aacuugucac gacaacagug ucaaacaugg ccgaaguaag auccuauugu uacgaagcau      840

caauaucgga uauggcaucc gauagcagau guccuacaca aggagaagca uauuuagaca      900

aacaaagcga uacacaauau guauguaaac gaacauuagu ugacagaggg ugggguaaug      960

gguguggacu auucgguaaa gguaguuuag ucacuugcgc aaaauuugca uguucuaaga     1020

aaaugacagg aaaaucaaua caaccggaaa accuagaaua cagaauaaug cuaucagugc     1080

auggaucaca acauagcgga augauaguaa augacacugg ucacgagaca gaugaaaaua     1140

gagcaaaagu agaaauaaca ccaaauucac caagagcgga agcaacauua ggagguuuug     1200

gaucauuggg auuagauugc gaaccaagaa caggauuaga uuuuucagau cuuuauuauc     1260

ugacaaugaa uaauaaacau uggcuagugc acaaagaaug guuccaugau auaccccuac     1320

cauggcaugc aggugccgau accggaacac cacauuggaa uaauaaagaa gcauuaguug     1380

aauuuaaaga ugcucacgcc aaaagacaaa caguaguagu auuaggcuca caagaagggg     1440

caguacauac cgcauuggcu ggagcguuag aagccgaaau ggacggugcc aaaggaagac     1500

ucucaagugg ucauuugaaa ugcagacuaa aaauggauaa auuaagauua aaaggaguau     1560

cauauaguuu augcacagcu gcauuuacau uuacaaaaau accagcagaa acucuacaug     1620

gaacaguuac aguugagguu caauaugcag gaacagaugg accaugcaaa gucccugcac     1680

aaauggcagu agacaugcaa acacugacac cuguaggaag auuaauaacu gcaaauccgg     1740

uaauaacaga aaguaccgaa aacucgaaaa ugauguuaga acuugauccu ccguucggag     1800

acagcuacau uguaauagga guaggagaaa aaaaaauaac gcaucacugg cauagaucag     1860

gaucgaccau cggaaaagca uuugaggcca cugugagggg agcaagaaga auggcaguau     1920

uaggcgauac agcuugggau uuugguucag ucggaggagc auugaauagu uugggaaaag     1980

gaauacauca aauauuugga gcagcauuua aaucauuauu uggugguaug agcugguucu     2040

cccagauauu aauaggaaca cuauuaaugu gguuagguuu aaauacuaaa aauggaagua     2100

uaucauuaau guguuuagcu uuaggaggcg uacuaauauu uuuaucaacg gcuguuucag     2160

cagauguggg augcucagua gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7006
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 2 opt

<400>  7006
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uaauuaaugc uagaaaagaa aagaaaagaa gaggagcaga aacaagugua ggaaucguug      120

guuuauuauu aacaacugcu auggcagccg aaguaacaag gagagggagu gccuauuaca      180

uguauuuaga cagaaaugac gcaggagaag caauuuccuu cccaacuacu uuagguauga      240

acaaauguua uauacaaaua auggauuugg gacauaugug cgaugcgaca augaguuaug      300

aauguccaau gcuagacgaa ggagucgaac cugaugacgu agacuguugg uguaacacca      360

caaguacaug gguaguauau ggaacgugcc aucauaagaa aggagaagcg agaagaagua      420

ggagagccgu aacauuacca agucacagca caagaaaauu acaaacaaga agucaaacau      480

gguuagaauc aagagaauau acaaaacacc uaauaagggu agaaaacugg auauuuagaa      540

auccaggauu ugcauuggca gcagcagcca uagcaugguu guuaggauca aguacaaguc      600

aaaaaguaau auauuuagua augauauuau uaauagcacc agcauauuca auaagaugca      660

uagguguauc aaauagagac uuuguagaag guaugagugg aggaacuugg guagauguag      720

uuuuagaaca cggaggaugc guaacaguaa uggcucaaga caaaccaaca guagauauag      780

aauuagucac uacaacagua ucaaauaugg cagaagucag aagcuauugu uacgaagcaa      840

gcauuucaga cauggcaucu gauucacguu guccuacgca aggagaagcc uauuuagaua      900

aacaauccga cacacaauau guauguaaaa gaacccuagu agacagaggu ugggguaaug      960

gauguggccu auucgggaaa ggaaguuuag ucacaugugc uaaauuugca ugcaguaaga     1020

aaaugacagg aaaaucaaua caaccggaaa aucuagaaua cagaauaaug cuaaguguac     1080

auggaaguca acauuccggc augauaguga augauacugg gcaugaaacc gaugaaaaca     1140

gagcaaaagu agaaauaaca ccaaauucuc cgcgugcuga agcuacacua gggggauuug     1200

gaucauuagg ucuugacugu gaaccaagga cugguuuaga uuucucggau cuauauuauc     1260

uaacuaugaa uaacaaacau uggcuuguac auaaagaaug guuucacgau auaccacuac     1320

cauggcaugc aggagcagau acagggacuc cucacuggaa caauaaagaa gcauuaguug     1380

aguuuaaaga ugcacaugca aaaagacaaa cuguaguugu auuaggcuca caagaaggug     1440

caguacacac agcucuagca ggggcucuag aggcggaaau ggauggugca aaaggaagac     1500

uuucaagugg acacuuaaaa ugcagacuaa aaauggauaa auugagauua aaaggaguaa     1560

guuauucuuu auguacagca gcauucacau uuacaaaaau accagcagaa acauuacaug     1620

gaacaguaac aguugaagua caauacgcgg gaacagaugg gccaugcaaa gucccagcgc     1680

aaauggcugu agacaugcaa acgcugacac ccguagggcg acuaauuacu gcaaauccag     1740

uaauaacuga aaguacagaa aacaguaaaa ugaugcugga auuagauccc ccguuuggag     1800

acagcuauau aguaauagga guaggugaaa agaaaaucac acaucauugg cauagaucag     1860

guucaacaau aggaaaagcc uuugaagcaa caguuagagg agccaaacgg auggcagugc     1920

uuggagauac ggcuugggac uucggaucag uaggaggcgc auuaaacagu uuagggaaag     1980

gaauacacca aauuuucggu gcagcuuuua aaaguuuauu uggaggaaug ucaugguuuu     2040

cucaaauauu aauaggaacu uugcuaaugu ggcuaggauu aaacaccaaa aauggaucaa     2100

uaucacugau gugucuagcu cuagguggug uauuaauauu ucuaucaaca gcaguuucag     2160

cagauguugg augcucaguu gauuuuucga aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7007
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 2 opt

<400>  7007
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc cagaaaagaa aagaaaagaa gaggugcaga uacauccgua ggaauaguag      120

gauuacuuuu aacaacagca auggcagcag aaguaacaag aagggguuca gcauauuaua      180

uguaccuaga uaggaaugac gcgggagaag cuauaucauu ucccacaacc cuaggaauga      240

auaaaugcua uauacagauc auggaccuug gacauaugug ugaugcaaca augucauacg      300

aauguccaau guuagaugaa ggaguagaac cggaugacgu cgauugcugg ugcaacacua      360

cgaguaccug gguagucuau ggaacauguc aucauaaaaa gggagaagca agacgaagua      420

ggagagcagu cacuuugcca agucacagua cuagaaaacu gcagacaaga agucaaacau      480

gguuagaauc aagagaauau acuaagcacc uaauaagagu ugagaauugg auauuuagaa      540

acccagguuu ugcucuugca gcugcggcua uagcguggcu guuaggauca ucaacaaguc      600

aaaaaguuau auacuuagug augauauuau uaauugcucc ugcauauuca cuaagaugua      660

uuggaguaag uaauagagau uuuguagaag guaugagugg aggcacgugg guugauguag      720

uacuagaaca uggcggaugu guuacaguaa uggcacaaga uaaaccuaca guagauauag      780

aauuaguaac uacuacagua aguaauaugg cagaaguuag aaguuacugu uaugaagcaa      840

guauaucaga uauggcaagu gauucaagau gucccacgca aggagaggcu uaucuagaua      900

aacaaucaga uacacaauac gucuguaaac gaaccuuagu agaucgaggu uggggaaaug      960

ggugcgguuu auucggaaaa ggaucauuag uaacuugugc aaaauuugca uguucaaaga     1020

aaaugacagg aaaaagcauc caaccagaaa acuuagaaua uagaauaaug uuaaguguac     1080

augguucuca acauucagga augauaguaa acgacacagg ccaugaaaca gaugaaaaua     1140

gagcaaaagu agaaauaaca ccaaauucac caagagcuga agcgacuuua ggaggauuug     1200

guagcuuagg uuuagauugu gaacccagaa caggauugga uuucagugau uuauauuauc     1260

uaacgaugaa uaacaaacau ugguuaguac auaaagaaug guuccaugau auaccauugc     1320

cuuggcaugc uggagcagau acaggaacgc cacacuggaa uaauaaagaa gcauuaguag     1380

aauuuaaaga ugcacacgcg aaaagacaga caguagucgu acuaggauca caagaaggag     1440

caguacauac agcauuggcc ggagcuuuag aagccgaaau ggauggagca aaaggcagau     1500

uaagcagugg ccacuuaaaa ugucgauuaa aaauggauaa auuaagauua aaaggaguca     1560

guuacagcuu auguacagcu gcauucacau uuaccaaaau cccagcagaa acauuacaug     1620

guacugugac aguagaagua caauacgcug gaacagaugg cccuuguaaa guaccagcac     1680

aaauggcagu agauaugcaa acauuaacac cgguaggaag guuaauuacu gcaaauccag     1740

uaauaacuga aucaacagaa aauaguaaaa ugaugcuaga auuagaccca cccuuugggg     1800

auaguuauau agugauagga guaggagaaa agaaaauaac acaccacugg caucguagcg     1860

gaaguacaau aggaaaagca uuugaagcua cgguaagagg ggcaaaacga auggcugugc     1920

uaggggacac agccugggau uucggaucag ugggaggugc auuaaauuca uuaggaaaag     1980

ggauacauca aauauucgga gcugcguuua aaucauuauu cggaggaaug aguugguuuu     2040

cucaaauauu aauuggaacg cuucuaaugu ggcuaggacu aaauacuaaa aacgguucaa     2100

uaucucugau guguuuagca uugggaggag uacuaauauu ccuuaguaca gcaguaagug     2160

cggauguagg cugcucagua gauuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7008
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 2 opt

<400>  7008
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaacgc aagaaaagaa aaaaagagaa gaggagcuga cacuaaugua ggaauaguag      120

gacuguuauu aacaacagcg auggcagcag aaguaacgag aagaggaucu gcauacuaua      180

uguauuuaga uaggaaugau gcaggagaag ccauaucauu uccgacaaca uugggaauga      240

auaaauguua cauacaaaua auggaucuag gacauaugug ugacgcaaca augaguuacg      300

agugucccau guuagaugaa gggguugagc cagacgaugu ggauuguugg uguaacacaa      360

caaguacuug gguuguauau ggaacuugcc accauaaaaa aggugaagcu agaagaucac      420

guagggcggu aacacuacca ucucacagua cccguaaacu acaaacgaga agccagacau      480

gguuagaaag cagagaauau acaaaacauu uaauaagagu cgaaaauugg auguuuagaa      540

auccaggcuu ugcauuagca gcagccgcua uagcauggcu ucuuggauca aguacaucac      600

aaaaaguuau auauuuagua augauauugu uaauagcccc agcuuacuca auaagaugua      660

uuggaguaag caacagagau uuuguagaag gaaugagugg aggaacaugg guugauguag      720

uacuagagca uggugggugc guaacaguca uggcgcaaga caaaccuaca guagauauag      780

aauuaguaac aacaacagua aguaauaugg cagaagugcg aucauauugu uacgaagcaa      840

gcauuucuga uauggcauca gauagucguu guccaacaca aggagaagca uaccuagaua      900

aacaaucaga uacccaauac guauguaaaa gaacuuuagu agaucgaggu ugggguaaug      960

gauguggacu auuugguaaa ggaagccuag uuacuugugc uaaguuugca ugcaguaaaa     1020

aaaugacagg uaaaucaaua caaccagaaa aucuagagua uagaauaaug uuaaguguac     1080

auggaaguca acauaguggu augauuguaa augauacagg acacgaaaca gacgaaaacc     1140

gagcuaaagu agaaaucacg ccaaauaguc ccagggcaga agcaacauua ggaggauuug     1200

gcucguuagg auuagacugu gaaccacgaa cugggcuaga uuuuagcgau uuauauuauc     1260

uaacaaugaa caacaaacac ugguuaguac auaaagaaug guuucaugau aucccacuac     1320

cuuggcaugc cggagcagac acaggaacac cacacuggaa uaauaaagaa gcacuaguag     1380

aguuuaaaga cgcacaugcg aaaaggcaaa caguaguagu acuaggauca caagaaggag     1440

cgguacauac ggcacuagcu ggagcccuag aagcagagau ggacggcgcu aaaggaagau     1500

ugagcagugg acaccuaaaa ugucguuuga aaauggauaa auuaagacuc aaaggaguaa     1560

guuauucguu augcacagcu gcguuuacau uuaccaaaau accagcagaa acgcuacaug     1620

gaaccguaac gguggaaguu caauaugcag gaacagaugg accaugcaaa guaccagcuc     1680

agauggcggu agacaugcaa acauuaacac caguagggag guuaauaaca gcaaacccug     1740

ugauaacuga aaguacagaa aauaguaaga ugaugcuaga acuagaucca ccauucggag     1800

auucauauau aguaauagga guaggggaaa aaaaaauaac ucaucauugg cauagaagug     1860

guaguacaau cggaaaagca uuugaagcaa caguacgcgg ggcaaaaaga auggcaguac     1920

uuggagauac agcaugggau uuuggaagug uaggaggugc guuaaauagu uuaggaaaag     1980

gaauacauca aauauucggg gcagcauuca aaaguuuauu uggaggaaug aguugguuca     2040

gucaaauauu aauaggaaca uuguuaaugu ggcuaggucu uaauacaaaa aaugguucaa     2100

uaaguuuaau gugucuugca uuaggaggag ucuuaauauu uuuaagcaca gcaguaagug     2160

cggacguugg guguucaguu gacuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7009
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 2 opt

<400>  7009
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc acgcaaagag aaaaaaagaa gaggagcaga uacaagugua ggaauaguug      120

guuuauuacu aacaacagca auggcagcug aagucacaag aagaggauca gcauauuaua      180

uguaccuaga cagaaacgau gcuggagaag cgauaucauu uccuacgaca cuugguauga      240

auaaaugcua uauucaaaua auggacuugg gacauaugug ugaugcaacc augucauacg      300

aaugcccuau guuagaugag gguguagaac cugacgaugu agauugcugg uguaacacaa      360

cuaguacuug gguaguuuau ggaacauguc accacaaaaa gggagaagca agaagaucaa      420

gaagggccgu aacucuccca ucacacagua caagaaaauu acaaacaaga ucacaaacgu      480

gguuagaauc aagagaauau acaaaacacu uaauaagagu ugaaaauugg auacuacgua      540

auccaggauu cgcacucgca gcugcggcua uagcauggcu cuuagguucg aguaccucuc      600

agaagguaau auacuuagua augauauuau uaauagcacc agcuuauucu auaagaugua      660

uaggagucag uaauagagau uuuguugaag gaaugucagg aggaacuugg guagacgugg      720

uacuggaaca cggaggaugc gugaccguua uggcgcaaga uaaaccaacg guagauauag      780

aauuaguaac aaccacaguu aguaauaugg ccgagguaag gucuuauugu uaugaagccu      840

cuauaucuga cauggcauca gacucaagau gcccuacaca aggagaagca uauuuagaua      900

aacaaucaga uacacaauau guauguaaac gaacacuagu agauaggggc ugggguaaug      960

gaugcggacu auucggaaaa ggaaguuuag ucacaugugc aaaauucgca uguucuaaaa     1020

aaaugacagg aaaaucaaua caaccagaaa auuuagaaua ucgaauuaug uuaucaguac     1080

auggaucaca acauucugga augauaguaa acgacacagg ccacgaaaca gacgaaaaua     1140

gagcaaaagu ugaaauaaca ccaaauaguc cucgagccga agcaacacuu ggagguuuug     1200

gaagucuagg auuagauugu gaaccaagaa caggauuaga cuucucggau cuauauuauc     1260

ugaccaugaa uaauaaacau ugguuagugc acaaagaaug guuucaugau auaccguuac     1320

cauggcaugc cggagcagau acaggcacac cacauuggaa uaauaaagaa gcacuaguag     1380

aauucaaaga ugcucacgcu aaaagacaaa cagugguagu auuagguagc caagaaggag     1440

caguacacac agcccuagca ggugcucuug aagcagaaau ggauggagca aagggaagau     1500

uaaguuccgg acaccugaaa ugcagacuaa aaauggauaa auuaagacua aaaggagucu     1560

cauauucauu gugcacagca gcauuuacuu uuacaaaaau uccagcagaa acguugcacg     1620

gaacaguaac uguagaagua caauacgcag gaacagaugg accguguaaa guaccagcac     1680

aaauggcagu ugauaugcaa accuuaacac caguuggaag auuaauaacu gcaaacccag     1740

uaauaacaga aaguacggaa aauucaaaaa ugauguuaga auuagauccu ccauucggag     1800

auucauacau cguaauagga auaggagaaa aaaaaauaac acaucauugg cacagaucgg     1860

gaucaacaau agguaaagca uuugaagcua caguaagagg agcaaaacgg auggcaguau     1920

uaggagacac ugccugggau uuuggaucag uagggggagc auuaaauuca cuaggaaaag     1980

gcauccauca aauauuuggu gcugcuuuua aaucccuauu cggaggaaug agcugguucu     2040

cacaaauacu uauuggaacu cugcuaaugu ggcuaggauu aaauacaaaa aauggaagca     2100

uaagcuuaau gugucuagcu cuaggaggcg uucuaauuuu uuuaaguaca gccguaucag     2160

cugacguagg auguucaguc gauuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7010
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 2 opt

<400>  7010
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauuaacgc uagaaaagaa aagaaaagaa gaggagcuga cacaagcgug ggaauaguag      120

gacucuugcu aacaacagca auggcugcag aaguaacaag aagaggaagu gcauauuaua      180

uguauuugga ucgaaaugac gcaggagaag caauaucauu uccaacaaca cucggaauga      240

auaaaugcua uauacaaaua auggaucucg gacauaugug cgacgccaca augucuuaug      300

aauguccuau gcuagaugaa ggaguagaac cagacgaugu cgauuguugg uguaauacaa      360

caagcacaug ggugguauac ggaacauguc aucauaagaa aggugaagca aggagaagua      420

gacgggcagu aacacuacca ucacauagua cgcgaaaacu acaaacaaga ucacaaacuu      480

ggcuagaauc aagagaauac acaaaacauc uaauuagagu agaaaacugg auauuuagaa      540

acccaggcuu cgcacuggca gcagcagcaa uagcaugguu gcuaggguca agcacaucac      600

aaaaaguuau auauuuagua augauauuau ugauagcucc agcauacagu auaagaugua      660

uaggaguguc aaaucgagau uuuguugaag gaaugucagg aggcacuugg guagauguag      720

uauuagaaca uggugguugc guaacaguaa uggcucaaga uaaaccaaca guagauaucg      780

aauuaguauc aacaacaguu ucaaacaugg cagaaguaag aucuuauugu uacgaagcaa      840

ccauaagcga uauagcaagu gauucaaggu guccuacaca aggagaagca uaucuagaca      900

aacaaucaga uacacaauac guaugcaaaa gaacauuagu ugauagagga uggggaaaug      960

gaugcggguu auuuggaaaa gggaguuuag uaacuugugc aaaauuugcg uguucaaaga     1020

aaaugacagg aaaaucaaua caaccagaaa acuuagaaua ucgaauuaug uugucaguac     1080

auggaucaca acauagcggg augauaguca augauacagg acaugaaacu gacgaaaaua     1140

gagcaaaagu agaaauaaca ccaaauagcc cuagagccga agcuacauua ggaggauuug     1200

guucacucgg acuagacugu gaaccaagga cuggucuuga uuuuucagau uuauauuacu     1260

uaacaaugaa uaauaaacac ugguuagucc acaaagaaug guuucaugac auaccauugc     1320

cuuggcaugc aggagcagau accggaacac cacacuggaa uaauaaagaa gcauugguag     1380

aauuuaaaga cgcucaugca aaaaggcaaa cagcuguugu acucggaucu caagaaggag     1440

caguucacac agcuuuagcu ggagcacuug aagcagaaau ggauggagca aaaggaagac     1500

uaucuucggg ccauuuaaaa ugcagacuaa aaauggauaa auugcgauua aaagggguua     1560

guuacaguuu auguacagcg gcuuuuacuu uuacaaaaau accagcagaa acacuacaug     1620

gaacaguaac aguagaagua caauaugcgg guacagaugg cccaugcaaa guaccagcac     1680

aaauggcagu agauaugcaa acacuaacgc caguaggaag gcugauuaca gcaaauccag     1740

uaauaacaga aucuacugaa aacucaaaaa ugaugcucga acuagaucca ccauuugggg     1800

auucuuauau cguuauaggc guaggcgaaa agaaaauuac acaccauugg cacagaucag     1860

gaucaacaau aggaaaagcc uuugaagcua caguaagagg ugcaaaaaga auggcuguuc     1920

uuggagacac agcaugggau uuugguagug uagggggagc auugaacuca cuagggaaag     1980

gcauacauca aauuuuuggu gcagcauuua aaucauuauu cggaggaaug agcugguuua     2040

gccaaauucu aauuggaacu uuacugaugu ggcuaggccu uaauacuaaa aauggaagua     2100

uaucacuuau gugcuuagca uuaggaggag uacugauauu uuuaucaaca gccguuucag     2160

cagacguugg uugcaguguu gauuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7011
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 2 opt

<400>  7011
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc ucguaaagaa aaaaagagaa gaggagcaga cacaagugua ggcauaguag      120

gauugcuuuu gacuacagca auggcagcag agguaacaag gagaggguca gcuuauuaua      180

uguacuuaga cagaaacgac gcaggugaag ccauauccuu uccgacaaca cuagguauga      240

auaaaugcua cauacaaauc auggauuuag gacauaugug cgaugcaaca augagcuacg      300

aauguccaau guuagaugaa ggaguagaac cagaugaugu ugauuguugg uguaacacaa      360

cuagcacaug gguagucuau ggcacauguc accacaaaaa aggcgaagcu agaagaucca      420

gacgagccgu aacauuaccu agucauagca cgagaaagcu acaaacaaga agccaaacau      480

gguuagaaag uagagaauau acaaaacauc uaaucagagu ggaaaacugg auauucagaa      540

accccgguuu ugcauuagcu gcagcagcca uagcauggcu auuaggaagu ucaacgagcc      600

aaaaaguaau auauuuagua augauauuac ugaucgcucc agcauauuca auacgaugua      660

uagggguaag caauagagac uucgucgaag gaaugucagg aggaacaugg guagauguug      720

ugcucgaaca cggugguugu gucacaguaa uggcacaaga uaaaccaacc gucgauauag      780

aacuaguaac aacaacaguu ucaaauaugg cagaaguaag aucuuacugu uaugaggcaa      840

guauaaguga uauggcguca gauucaagau gcccaacuca aggagaagca uauuuagaua      900

aacaaaguga uacacaauac guauguaaaa gaacuuuagu agauagagga uggggaaaug      960

gcuguggucu auuuggcaaa ggaagucuag uaacuugugc aaaauucgca ugcagcaaaa     1020

aaaugacagg aaaaagcaua caacccgaaa acuuagaaua ucgaauaaug cugucagucc     1080

auggaaguca acacaguggu augauaguua augauacugg acaugaaaca gacgaaaaua     1140

gagcgaaagu agagauaacu ccaaauucgc ccagagcaga agcuacccua ggagguuuug     1200

gaucacucgg uuuggauugc gaaccucgaa cagguuuaga cuuuagcgac uuauauuacc     1260

uaacaaugaa caauaaacau uggcuaguac acaaagaaug guuucaugau auaccuuuac     1320

cauggcaugc uggcgcagau acaggaacac cgcauuggaa uaauaaagaa gcacuaguag     1380

aauuuaaaga ugcacaugcc aaaagacaaa cugucguugu auuagguaca caagaaggag     1440

cuguacauac agcauuagcc ggagcauuag aagcagagau ggacggugca aaaggccgau     1500

uaaguucagg gcaucuaaaa uguagauuga aaauggacaa auuacgacua aaaggaguuu     1560

cauauucauu auguaccgca gcauucacuu uuacaaaaau accugcagag acauuacaug     1620

guacaguaac aguagaagua caauaugcag gaacagacgg accauguaaa guaccugcuc     1680

aaauggcagu agauaugcag acucuaacac cagucggcag auuaauaaca gcaaaucccg     1740

uaauaacaga aucgacggaa aauucuaaaa ugauguuaga acucgauccu ccauuuggag     1800

auaguuacau aguaauuggg guaggagaaa aaaaaaucac ucaucauugg cauagaagug     1860

gaucgacgau aggaaaagca uucgaagcaa caguacgagg agcuaaaaga auggcuguuu     1920

uaggugacac ugcaugggac uuuggaucag uuggaggagc guuaaauagc uuaggaaagg     1980

gaauacauca gauauuugga gcagcguuca aaagucucuu cgguggaaug ucaugguuca     2040

gucaaauacu gauagguacc uuauuaaugu ggcuaggauu aaauacaaaa aacgguucca     2100

uuagucuuau guguuuagca uuaggcggag uauuaauauu cuuaucaaca gcaguaucgg     2160

cagacguagg auguagugua gauuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7012
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 2 opt

<400>  7012
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc ccguaaagaa aagaaaagaa gaggugcaga uacaucagug gguaucguag      120

guuuauuauu aacaacagcg auggcagcgg aaguaacaag aagaggcuca gcauauuaua      180

uguaccuaga cagaaacgau gcgggagaag caauaucuuu uccuacaaca cuuggaauga      240

auaaaugcua uauacaaauu auggacuuag gacacaugug ugaugcuaca augagcuacg      300

aauguccaau guuagaugaa ggaguagaac cagaugacgu cgauugcugg ugcaacacua      360

cgagcacaug gguuguuuac gguacaugcc accauaagaa aggagaagca cgaagaucaa      420

gaagggcugu cacguuaccg ucacauucaa caagaaaauu gcaaacaaga ucacaaacau      480

gguuagaguc aagagaauau acaaaacauu uaauuagagu agaaaauugg auuuuuagaa      540

auccagguuu ugcacuugca gcagcugcua uagcaugguu auuaggauca agcacaucac      600

aaaaagucau auauuuagug augauuuuac uaauugcacc cgcauacagc auaagaugua      660

uaggaguauc aaauagagac uucguagaag gaaugucggg aggaacuugg guagauguag      720

uauuagaaca ugggggaugu guaacaguaa uggcucagga uaaaccaacu guugauauug      780

aauugguaac aacaacagug aguaauaugg cugaaguaag aaguuauugu uacgaagcaa      840

guauaaguga uauggcaagu gauucaaggu guccuacuca aggagaggca uacuuagaca      900

aacaaucuga cacucaauau guauguaaac gaacauuagu cgaucgaggu ugggguaaug      960

gauguggucu auuuggaaaa ggaucgcuag uaacaugugc caaauuugca uguaguaaga     1020

aaaugacagg aaaaaguauu caaccagaga acuuagaaua ucgaauuaug uuaucaguuc     1080

auggaaguca acauagugga augauaguaa augacaccgg ucaugaaacg gaugaaaaua     1140

gagcaaaagu ugagauaaca ccaaauaguc cuagagcaga agcaacacua ggagguuucg     1200

gaagucuagg uuuagauugu gaaccacgaa caggauuaga cuuuagcgau cuauacuacu     1260

uaacuaugaa caauaaacac ugguugguac acaaagaaug guuucaugac auaccacuac     1320

cguggcacgc uggagcugau acuggaacac cacauuggaa uaauaaagaa gcauugguag     1380

aguuuaaaga ugcacacgca aaaagacaaa caguaguagu auuaggaagc caagaaggag     1440

caguacauac cgcauuggcu ggugcauuag aggcagaaau ggauggugcu aaagguaggu     1500

uaaguucagg ccauuuaaaa uguagauuaa aaauggauaa auuaagacua aaaggugucu     1560

cauacagcuu gugcacagca gcauucacau uuacaaaaau cccugcagaa acauugcaug     1620

gaacgguaac cguagaauua caauaugcag gcacagaugg accuuguaaa guaccagcuc     1680

aaauggcagu agauaugcaa acuuuaacuc caguaggaag auuaauaacu gcaaauccag     1740

ugauaacgga aaguacugaa aacaguaaaa ugauguuaga acuugauccg ccauuuggag     1800

acagcuacau agugauaggu gugggagaaa agaaaauaac ccaucauugg caccgaucag     1860

gaucaacaau aggaaaggcu uucgaagcaa caguuagagg cgcaaaacgc auggcuguau     1920

ugggagauac ugcaugggau uuuggaagug uagguggggc auuaaacagu uuaggaaaag     1980

gaauacauca aauuuucggc gcagcauuua aaucauuauu cggaggaaug agcugguuuu     2040

cacaaauacu uauaggaaca cuacuaaugu gguuagguuu aaauacuaaa aacggaagua     2100

uuucauuaau guguuuagca cucggaggug uauuaauauu ccuaucaaca gcaguaucag     2160

cagacguagg augcagcgua gacuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7013
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 2 opt

<400>  7013
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa aagaaaagaa gaggagcaga aacaagugua ggaaucguug      120

gauuauuauu aacaacagca auggcagcag aaguaacgag acggggauca gcuuauuaca      180

uguauuugga uagaaaugau gcaggagaag cuauuagcuu uccaacaaca cuggguauga      240

auaaauguua uauacaaaua auggacuugg gucauaugug cgacgcaaca augucauaug      300

aaugucccau guuagaugaa ggaguagaac cagacgaugu agauuguugg uguaauacaa      360

caaguacaug gguaguauau gguacgugcc aucauaaaaa gggagaagca aggagaucaa      420

gaagggcagu aacauuaccu agccauagca cucgaaaauu acaaacaaga agucagacgu      480

gguuggaauc aagagaguau acaaaacacu uaauacggau agaaaauugg auauucagaa      540

acccagguuu cgcucuggcu gccgcagcaa uugccuggcu auuaggguca agcaccucac      600

agaaagugau uuacuuagua augauacuau uaauagcucc ugcauacagc auuagaugua      660

uaggcguuuc aaaccgagac uuugucgaag gaaugucggg agguacaugg gucgauguag      720

uauuagagca cggaggaugu gugacaguaa uggcacaaga caaaccaaca gucgacauag      780

agcuaguaac aacaacugua aguaauaugg cagaaguuag aucauauugu uaugaagcca      840

guauaaguga uauggcuagu gauuccagau guccaacaca aggagaagca uaucuugaua      900

aacaaagcga uacacaauau guuuguaaaa gaacuuuagu agauagggga ugggguaacg      960

gguguggacu auuuggaaaa ggaucuuuag uuacaugugc aaaauuugca uguaguaaga     1020

aaaugacagg aaaaucaaua caaccagaaa auuuagaaua uagaauuaug uugucaguac     1080

augguucaca acauaguggu augauaguaa augacaccgg gcaugaaaca gacgaaaaua     1140

gagcaaaagu agaaauaacc ccaaauagcc cgagagcaga ggcaacauug gguggauuug     1200

gaagccuugg acuagauugu gaaccaagaa cagggcuaga uuucagugac cuguacuauu     1260

uaacaaugaa caacaaacau ugguuagucc auaaagagug guuccaugau auccccuuac     1320

cauggcacgc aggagcagac acgggaacac cacacuggaa caauaaagaa gcauuagucg     1380

aauuuaaaga ugcacaugca aaaagacaaa ccguaguggu auuaggauca caagaaggug     1440

caguucauac agcuuuagcu ggagcacuug aagcagaaau ggauggcgca aaaggaagau     1500

uaucaagugg acauuuaaaa uguagauuaa aaauggauaa auugagacua aaagguguau     1560

cauacaguuu auguacagca gcauuuacuu uuacaaaaau accugcagaa acauuacacg     1620

guacaguaac aguagaagua caauaugcag gaacagacgg accaugcaaa gucccggcac     1680

aaauggcugu agauaugcaa acacuaacac cagugggaag auuaauuaca gcaaauccag     1740

uaauaacaga aucaacugaa aauucgaaaa ugaugcuaga auuggaucca ccauuuggag     1800

acaguuauau aguaauuggu guuggcgaaa agaaaauaac acaucauugg cauagaucag     1860

gaucaacuau aggaaaagca uuugaggcua cagugcgagg agcuaaacga auggcagugu     1920

uaggugauac ggcaugggau uuuggaagug uaggcggugc ccuaaauuca cuuggaaaag     1980

gcauacacca aaucuuugga gcagcauuca aaucgcuauu ugguggaaug aguugguuua     2040

gucaaauauu aaucgggacu uuauuaaugu gguuaggauu aaauacuaaa aauggaagca     2100

uaucacuaau guguuuagcg uuaggaggag uauuaauuuu cuuaagcaca gccguaucag     2160

cagauguggg augcagugua gauuucucga aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7014
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 2 opt

<400>  7014
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauaaaugc uagaaaagaa aaaaaaagac ggggagcaga uacaucagua ggaauagugg      120

gauuauuacu aacaacugca auggccgcag aaguuacacg aagagggagu gcauauuaua      180

uguaucuaga ccgaaaugau gcaggagaag caauaagcuu uccaacaacc cuuggaauga      240

auaaauguua uauacaaaua auggauuuag gacauaugug ugacgcaaca augucauaug      300

aaugcccgau guuagacgaa gggguagaac cagacgaugu agacugcugg uguaauacaa      360

caucaacaug gguuguauac ggaaccugcc aucauaaaaa aggagaagca agaagaagua      420

gaagggcggu aaccuuaccu ucucauucaa cuagaaaacu ucaaacuaga ucacaaacuu      480

gguuagaaag uagggaauau acaaaacauu uaauaagagu agagaauugg auuuuuagaa      540

auccaggauu ugcauuagca gcagcagcaa uagcaugguu acucgguuca ucaacaaguc      600

aaaaaguaau uuacuuagua augauuuuac uaauugcacc cgccuacuca auuagaugua      660

uaggaguauc aaauagagau uucguagaag gaaugagugg aggcacuugg guugauguag      720

uauuagaaca ugguggcugu guaacaguca uggcacaaga uaagccaaca guagauauag      780

aacuaguaac aaccaccgua ucaaacaugg ccgaaguacg aucguacugu uaugaagcau      840

caauuaguga cauggcauca gauaguagau gucccacaca aggagaagca uaucuagaua      900

aacaaaguga uacucaauau guauguaaaa gaacauuagu agaucgaggu ugggguaaug      960

gauguggacu auuuggaaaa ggaucucuag ucaccugugc aaaauuugcu uguucaaaaa     1020

aaaugacagg aaaaagcauu caaccagaaa auuuagaaua uagaauuaug uuaucaguac     1080

auggaagcca acauucugga augauaguaa augauacugg acacgaaaca gaugagaaua     1140

gggcaaaagu ugaaauaaca ccaaauucac cuagagcaga agcaacauua ggcgguuuug     1200

guagcuuggg uuuggauugu gaaccuagaa caggacuaga uuuuagugau uuauauuauc     1260

uaacaaugaa uaauaaacau uggcugguac auaaagaaug guuucaugau auaccauuac     1320

cuuggcaugc aggagccgau accgguacgc cucauuggaa caauaaagaa gcacuuguag     1380

aauucaaaga ugcacacgca aaacgucaaa caguaguagu auuaggauca caagaagggg     1440

caguacauac ggcguuagca ggcgcauuag aagcagaaau ggauggagcu aaaggucguc     1500

uuucaagugg acauuuaaaa uguagguuaa aaauggauaa acuacgauua aaaggcguau     1560

cuuauucauu auguacagca gcauuuacuu uuaccaaaau accagcagag acccuacaug     1620

gaacagucac gguagaagua caauaugcug guacugaugg accauguaaa guaccugcuc     1680

aaauggccgu agauaugcaa acauuaacac cagugggaag acuaauaaca gcaaauccgg     1740

uuauuacaga aagcauagaa aauucaaaaa ugauguuaga acuagaucca cccuucggug     1800

auucauauau aguuauaggc guaggggaga aaaaaauaac gcaucacugg caucgaucag     1860

gaaguacaau aggcaaagca uuugaagcua cuguacgugg agcaaaacga auggccguuc     1920

ucggggacac ugcuugggac uucggcucag uaggaggggc gcuaaauagu cucggaaaag     1980

gcauacacca aauuuuugga gcagcuuuca aaucauuauu cggaggaaug ucgugguuca     2040

gucaaauauu aaucggaaca cuacuaaugu ggcuaggacu caauaccaaa aaugggagua     2100

uaaguuuaau gugucuagca uuaggaggug uuuuaauauu uuuaucaacu gcuguuucag     2160

cagacguagg augcucaguu gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7015
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 2 opt

<400>  7015
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc aaggaaagaa aagaaaagaa gaggagcuga uacaucugua ggaauuguag      120

gguuacuauu aacaacagca auggcugcgg agguaacaag gaggggcuca gcauacuaua      180

uguaucuaga ccgaaaugau gcaggagaag caauaaguuu cccaacaaca uugggaauga      240

auaaauguua cauacaaaua auggauuuag gccauaugug cgaugcaaca augaguuaug      300

aaugcccgau gcuagaugaa ggaguugaac ccgaugaugu cgauuguugg uguaauacaa      360

caaguacuug gguaguauac ggaacauguc aucauaagaa aggugaagcu agaagaucaa      420

ggagagcggu aacacuaccg ucacauucaa ccagaaaauu acaaacaaga ucacaaaccu      480

gguuagaaag uagagaauau acaaaacauu uaauaagagu agaaaacugg auauuuagaa      540

auccagguuu ugcauuggcu gcugcagcua uagcaugguu acuaggaagc ucaacaaguc      600

agaaaguaau auaucuaguc augauuuuac uaaucgcacc agccuacagu auaaggugua      660

uaggaguauc aaauagagac uuuguagaag guaugagugg agguacaugg guagacguag      720

uacuagaaca cggagguugu guaaccguaa uggcccaaga uaaaccgaca guagacauag      780

agcuaguaac aacaacaguu ucaaauaugg cugaaguuag aagcuauugc uaugaagccu      840

caauaagcga cauggcuagu gauucaagau gucccacaca gggagaagca uauuuagaua      900

aacagucaga cacucaauau guauguaaga gaacauuagu agacaggggu uggggaaaug      960

gauguggauu auucggaaaa ggaagucuug uaacaugcgc aaaauuugca uguucgaaga     1020

aaaugacagg aaaaaguaua caaccagaaa acuuagaaua uagaauaaug cuuaguguac     1080

auggaaguca acauagugga augauaguua augauacugg ucaugaaaca gaugaaaaua     1140

gagcaaaagu agaaauaacu ccaaacagcc cuagagcaga agcuacauua ggaggauuug     1200

guaguuuagg acuagauugc gagccuagaa ccgggcugga cuuuucugau uuauauuacu     1260

uaacaaugaa caauaaacau uggcuaguac auaaagaaug guuccaugau auaccuuugc     1320

cauggcacgc aggggcagau acaggaacac cucauuggaa uaacaaagaa gcucuaguag     1380

aauuuaaaga ugcacaugca aaaagacaaa caguuguugu auuaggaagu caagaaggag     1440

caguacacac agcacuagca ggagcauuag aagcagaaau ggauggagua aaaggccggc     1500

uuagcagcgg ucaucuaaaa uguagauuaa aaauggacaa acuaagguua aaagguguaa     1560

guuauagucu guguacugcg gcauuuacau uuacaaaaau accagcagaa acauuacaug     1620

guaccguaac aguagaagua caauacgcag gaacggaugg acccuguaaa guuccugcac     1680

aaauggcagu agacaugcaa acacuaacac cuguggguag gcuaauaacu gcaaauccgg     1740

uaauaacaga aucaacugaa aauaguaaaa ugaugcuuga auuggauccc ccuuuuggag     1800

acaguuauau aguaauaggg guaggagaaa agaaaauaac ucaccacugg cauagaagcg     1860

gaucaacuau agggaaagcg uucgaagcaa caguacgagg cgcaaaaaga auggcaguau     1920

uaggagacac cgcaugggau uuugguagug uaggaggugc acuuaauagc cuaggcaaag     1980

gcauacacca aauauucgga gcugcauuua aaucauuauu uggagggaug agcugguucu     2040

cacaaauauu aaucggaaca uuauuaaugu ggcuuggacu aaauaccaaa aauggaucaa     2100

uaucauuuau gugucuagca cuuggcggag uauuaauuuu ucuaaguaca gcaguuucag     2160

cagacguagg cuguagugua gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7016
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 2 opt

<400>  7016
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgua       60

uaauaaaugc acgaaaagaa aaaaaaagaa gaggagcuga cacaucaguu ggaauuguag      120

gauuauuauu aacuacagca auggcagcag aaguaacaag acgaggaucu gcuuauuaua      180

uguauuuaga uagaaacgau gcgggagaag caauaucauu ucccacaaca uugggaauga      240

auaaauguua cauacaaauu auggauuuag gacauaugug ugaugcaaca augagcuaug      300

aauguccaau gcuagacgaa ggaguugaac cugaugaugu agacuguugg ugcaauacua      360

caucaacaug ggucguauau ggaacaugcc aucacaaaaa aggugaagcu agaagaagua      420

gaagagcagu aaccuuacca ucccacagua cacgaaaacu acaaacaagg agucaaacau      480

gguuagaauc aagagaauau accaaacauc ugauacgagu agaaaauugg auauucagaa      540

acccaggauu cgccuuggca gcugccgcga uagcauggcu acuaggaagu ucaacuucac      600

aaaaaguaau auacuuaguc augauacuac uuauugcgcc agcauauagc auacgaugua      660

uaggagucag caauagagau uuugucgaag gaaugucugg aggaacaugg guagacguag      720

uauuagaaca uggaggaugu guaacaguaa uggcacaaga uaaaccaacu guagauauug      780

aauuaguuac cacaacagua ucaaauaugg cagaaguaag aucauacugu uaugaagcaa      840

guauaagcga uauggcaagu gacucaagau guccaaccca aggagaagca uacuuagaua      900

aacaaagcga uacacaauau guauguaaac gaacguuagu agacagagga uggggaaaug      960

gauguggucu auucggaaaa gguaguuuag ugacaugugc aaaauuugcu uguaguaaaa     1020

aaaugacagg aaaauccauu caaccagaaa aucuagaaua uagaauaaug cugaguguac     1080

auggaucaca acacucuggu augauaguca acgacacugg acaugaaacu gaugaaaaua     1140

gggcuaaagu agaaauaaca ccuaauucac caagagccga agcuacuuua ggugguuuug     1200

gcucauuagg cuuagauugu gaaccacgaa caggauuaga uuuuucagac cuauacuacc     1260

uaacaaugaa caacaaacau ugguuaguuc auaaagaaug guuucacgau auaccacuac     1320

cuuggcaugc aggugcagau acuggaacuc cacauuggaa uaacaaagaa gcacuagucg     1380

aauuuaaaga cgcacaugca aaacgacaaa caguaguagu uuugggcucu caagaaggug     1440

caguucauac agcacuagcu ggugcucuag aagcagaaau ggauggagcu aaggguaaac     1500

uaaguagcgg acauuuaaaa ugcagacuaa aaauggauaa auuacgacua aaaggaguaa     1560

guuauucauu augcacagcg gcauucaccu uuacaaaaau accagcagaa acauuacacg     1620

gaacaguuac aguugaaguc caauaugcag gaacagaugg accauguaag guaccugccc     1680

aaauggcugu agauaugcaa acacuuacac cuguuggaag acuuauuaca gcuaaccccg     1740

uaauaaccga gucaacagaa aacaguaaaa ugaugcuaga acuagacccc ccauuuggug     1800

auaguuauau agugauaggg guaggugaaa aaaaaauaac acaucauugg cauaggagcg     1860

gaucaacaau agguaaagca uuugaagcca caguaagagg ugccaaaaga auggcagugu     1920

uaggagacac agcaugggac uucggaucag ugggcggugc acuaaacagu uuagguaagg     1980

gaauacauca gauauuuggu gcugcauuua aaucuuuauu uggaggaaug aguugguuua     2040

gucaaauuuu aauuggaaca cuauuaaugu ggcuagggcu aaacacgaaa aacggcucaa     2100

uaucauuaau gugcuuagca uuagguggag uuuuaauauu ucuaucaaca gcaguuagug     2160

cugacguugg augcuccgua gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7017
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 2 opt

<400>  7017
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc uagaaaagaa aagaaaagaa gaggagcuga uacaaguguc gguaucguug      120

gacucuuauu aacaacugca auggccgcag aagugacacg aagggguagu gcauauuaca      180

uguauuuaga uagaaaugau gcaggagaag cgauaucauu ccccacaaca cuaggaauga      240

auaaaugcua uauacaaauu auggauuuag gucacaugug ugacgcaaca augucauaug      300

aauguccaau guuagacgaa ggaguagaac cagaugaugu agauugcugg uguaauacaa      360

caucaacaug ggucguauac ggaacuuguc accacaagaa aggagaagcu aggagaagua      420

gacgagcagu aacucuaccu agucauucua ccagaaaacu ccaaaccaga ucacaaacau      480

gguuggaauc aagagaauau acuaaacacc ugauaagggu agaaaauugg auauuucgua      540

acccagguuu cgcauuagca gcagcagcaa ucgcaugguu auuaggaagc agcacaucac      600

aaaaaguaau auauuuagug augauauuac uaauagcgcc ugcauauagu auuagaugua      660

uaggaguauc aaaucgagac uuuguugaag gaaugucagg aggaacaugg guagacguag      720

uucuugaaca cggaggaugu guaacaguaa uggcacaaga caaaccaacg guagauauag      780

aauuaguaac aacgacagua ucaaacaugg cagaaguaag aucuuacugu uacgaagcgu      840

ccauaucaga uauggcaagc gauucaagau gcccaaccca aggugaagca uaucuagaua      900

aacaaaguga uacacaauau guauguaaaa ggacucuagu cgacagaggg ugggguaaug      960

gauguggacu auuuggaaaa ggaucauuag uaacaugugc aaaauuugca uguucaaaga     1020

aaaugaccgg aaaaagcauc caaccagaaa aucuugaaua ccgaauuaug cuaucaguac     1080

auggaucaca acacucagga augauaguaa augacacagg ucaugaaaca gaugaaaaca     1140

gggcaaaagu agaaauaaca ccaaauucac cuagagcaga agcaacccuc ggagguuuug     1200

gaucauuagg auuagauugu gaaccuagaa caggacuaga uuuuucagac uuauacuacu     1260

uaaccaugaa caauaaacau ugguuaguac acaaagaaug guuucaugac auaccuuugc     1320

cauggcacgc aggagccgau accggaacac cacacuggaa caauaaagag gcuuuaguag     1380

aauuuaaaga agcacaugca aaacgacaaa caguaguagu auuaggaagu caagaagggg     1440

ccguacacac agcacuagca ggagcauuag aagcagaaau ggauggagcu aaaggaagac     1500

uaucaagugg acauuuaaaa ugcagacuaa aaauggauaa acuaagacua aaagggguca     1560

gcuacagucu auguacagcc gcauuuacau uuaccaaaau accagcagaa acacuacacg     1620

gaacaguaac aguagaagua caauacgcag gaacugacgg accauguaaa guacccgcac     1680

aaauggcagu agauaugcag acauuaacuc cagucggcag auuaauaacu gcaaacccug     1740

ucauaacaga aaguacagaa aauucaaaga ugauguuaga acuagaucca cccuucggcg     1800

acagcuauau cguuauagga gugggagaaa agaaaauaac acaucauugg caucgaucag     1860

guaguacaau aggaaaggcu uuugaagcaa caguaagagg ugccaaaaga auggcuguac     1920

uuggagauac agcaugggau uuuggaucag uagguggugc uuuaaauagu uuaggaaaag     1980

gaauacauca aauuuuugga gcagcauuua aaucauuauu uggaggaaug ucaugguucu     2040

cacaaauauu aauaggaacu cuauuaaugu ggcucggauu aaauaccaaa aaugggucaa     2100

uaaguuuaau gugccuagca uuagggggug uauuaauauu uuuaucaaca gcaguaagug     2160

cagauguugg auguucaguc gauuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7018
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 2 opt

<400>  7018
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaaugc uagaaaagaa aagaaaagaa gaggagcaga uacuucaguu ggaauaguag      120

guuuacuucu caccacugca auggcagcag aaguaacacg uagaggcuca gcauacuaca      180

uguauuuaga ucgaaaugau gcaggagaag caauaucauu cccaacaaca cucggaauga      240

auaaauguua uauacaaauc auggauuugg gacauaugug ugaugccacu augagcuacg      300

aauguccuau gcuagacgaa ggaguagaac cugaugaugu agauuguugg uguaacacua      360

caagcacaug gguaguuuau ggcacauguc aucauaagaa aggugaagca agaagaucaa      420

gacgagcggu uacacugccu ucucacucua caagaaaacu acaaacaaga ucacaaacuu      480

gguuagaaag uagagaauac acgaaacauu uaauaagagu agaaaacugg auuuucagaa      540

aucccggauu ugcuuuagcu gcagcagcua uagcuugguu auuaggaagc aguacaagcc      600

aaaaaguaau auauuuagua augauucuac uuauagcucc agcauauuca auaaggugua      660

uaggaguuuc aaauagagau uuuguagaag gaaugucagg uggaacaugg guagaugucg      720

uucuagaaca uggcggaugc guaacaguca uggcacaaga uaaaccaaca guagauauug      780

aauuaguaac aacaacugua aguaauaugg cagaaguaag aucguauugc uacgaagcau      840

caauaaguga uauggcauca gacucaagau gcccaacaca aggagaagca uaucuagaua      900

aacaaucaga cacgcaauau guuuguaagc gaaccuuagu agauagaggu ugggguaacg      960

ggugcggauu auuuggaaaa ggcagucuag uaacaugcgc uaaauuugcg uguaguaaaa     1020

agaugacugg aaaaucaauu caaccagaaa aucuagaaua uagaauaaug cugucaguac     1080

augguucaca acauagugga augauaguaa augacacggg ucacgaaaca gaugaaaaua     1140

gagcgaaagu agaaauaaca ccaaauagcc cacgagcuga agcuacauug ggagguuucg     1200

guucuuuagg uuuagacugc gaaccgagaa caggauuaga cuucagcgau cuauauuauu     1260

uaacaaugaa uaauaaacac ugguuaguac auaaagaaug guuucaugac auuccauuac     1320

cauggcaugc aggagcagau acagguacac cacauuggaa caauaaagaa gcauuaguag     1380

aauuuaaaga ugcacaugca aaaagacaaa caguaguagu uuuagguagu caagaaggag     1440

caguucacac agcacuagcu ggugcauuag aagcugaaau ggauggugcg aaaggaagac     1500

ugaguuccgg acaccuaaaa ugcagacuaa aaauggacaa acuaagacua aaagguguau     1560

cauacagcuu auguacagca gcauucacau ucacaaaaau ucccgcagaa acauuacaug     1620

gaacaguaac aguugaaguc caguacgcgg gugcagaugg uccuugcaaa guacccgccc     1680

aaauggcagu cgacaugcaa acuuuaacac cuguaggaag auugauaaca gcaaauccag     1740

uaauaacaga aucaacagaa aacaguaaaa ugauguugga acuugaucca ccauucggug     1800

acaguuacau aguaauaggg guuggagaaa agaaaauaac acaucauugg cauagaagug     1860

gaagcacaau aggaaaagca uuugaagcaa caguaagagg cgcaaaacgc auggcaguac     1920

uuggagauac agcuugggac uuugggagcg uuggaggagc ccuaaacagu cuuggaaaag     1980

gaauacauca gauauucgga gcugcauuca aaagucuauu uggaggaaug ucaugguuua     2040

gucaaauauu aaucgguaca uuauuaaugu ggcuaggcuu aaauacgaaa aauggaagua     2100

uaagucuuau gugucuagca uuagguggug uacuaauauu uuuaaguacu gcagugagug     2160

cagacguagg uuguucaguu gacuucagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7019
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 2 opt

<400>  7019
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc aggcaaagaa aaaaaaagac gaggugcaga uacuaacgug ggaauaguag      120

gauuauuacu aacaacagcg auggcagccg aaguaacuag acgaggaagc gcauauuaua      180

uguaucuaga ucgaaaugau gcgggagaag caauaaguuu cccaacaaca uuaggaauga      240

acaaaugcua uauacaaauc auggaucuug gacacaugug ugaugcaaca augagcuaug      300

aauguccaau guuagaugaa ggaguugaac cugaugacgu ggauuguugg ugcaacacga      360

caucaacaug gguaguauau ggaacaugcc aucacaaaaa aggcgaagcc agaagaagca      420

gaagagcagu aacguuaccu agucauagca caaggaaauu acaaacaaga agucaaacuu      480

ggcuugaauc aagagaauac acaaagcauc ucaucagagu ugaaaacugg aucuuucgaa      540

auccaggauu ugcuuuagca gccgcagcca uagcaugguu acuaggauca ucaacaaguc      600

aaaaaguaau auacuugguc augauacuac uaauugcacc agcuuauagu auacguugca      660

uaggaguaag uaacagggau uuuguagaag gaaugucugg aggaacaugg guugauguag      720

uccuagaaca cggaggaugu guuacgguaa uggcacaaga uaaaccaaca guagauauag      780

aauuaguaac caccacaguu ucaaauaugg cagaaguacg aucguauugu uacgaagcua      840

guauaaguga cauggcauca gauucaagau gcccaacaca aggagaagcc uauuuagaua      900

aacaaaguga cacacaauac guauguaaaa gaacacuagu agauagggga uggggaaaug      960

guugcgguuu auuugggaag gguaguuuag uaacgugugc aaaauuugca uguaguaaaa     1020

agaugacugg caaaagcauc caaccugaaa auuuagaaua uagaauaaug uuaucaguac     1080

auggaaguca acacucagga augaucguaa acgauacugg acacgaaacu gaugaaaauc     1140

gagcaaaagu agaaauuaca ccaaacucuc cacgagcaga agcaacauua ggaggauuug     1200

gaucauuagg ccuagauugc gaaccccgaa caggacuaga uuucagcgau uuauauuauu     1260

uaacaaugaa uaauaaacau ugguuaguac auaaagagug guuucaugac auaccucuac     1320

cguggcaugc aggagcagau acugguacac cacauuggaa uaauaaagaa gcacuggucg     1380

aauucaaaga ugcacaugca aaacgacaaa caguaguagu auuaggcagu caagaaggag     1440

cgguacauac agcacuagca ggagcccuag aagcagaaau ggacggugca aaaggaagau     1500

uaaguagugg acaucugaaa uguagauuaa aaauggauaa acuaagauua aaaggagucu     1560

cauauucauu auguacagca gcauuuacau uuacaaaaau acccgccgaa acauuacaug     1620

gaacaguaac aguggaagua caauaugcug gaacagaugg accuuguaaa guuccagcuc     1680

aaauggcagu agacaugcaa acauuaacac ccguagguag acuaauuaca gcaaacccug     1740

uaauaacaga aagcacagaa aacucaaaaa ugaugcuaga auuagauccc ccauuuggug     1800

auaguuauau uguuauagga guuggagaaa aaaaaauaac acaucauugg cacagauccg     1860

guucgacaau uggaaaagca uuugaagcua cuguaagagg ugcaaggcgg auggcgguau     1920

ugggagacac ugcuugggac uucggaucag uuggaggagc acuaaacuca cuaggaaaag     1980

guauacauca aauuuuuggu gcagccuuua aaucauuauu cggaggcaug uccugguucu     2040

cacaaauacu aauagguaca cuucuaaugu gguuaggacu aaauacgaaa aauggaucaa     2100

uaagcuuaau guguuuagcc cuagggggag uccuaauauu cuuaucaacu gcaguaucag     2160

cagauguagg uuguagugua gauuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7020
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 2 opt

<400>  7020
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa aaaaaacgcc gaggagcaga aacuagugua gggauaguag      120

guuuacuuuu aacaacagca auggcagcag aaguuacaag aagaggaagu gcuuauuaua      180

uguauuuaga ccgcaaagau gcaggagaag caauaaguuu ucccacaacg uuggguauga      240

auaaauguua cauacaaauu auggacuuag gacacaugug cgaugcaacu augagcuacg      300

aaugcccuau gcuagaugaa ggggucgaac cagaugacgu ugauuguugg uguaacacaa      360

caucaacaug gguagucuau gguacaugcc aucauaaaaa aggugaagcu cgacgaucaa      420

gaagagcagu aacacuaccu agucauagua cuagaaaacu acaaacaaga agucaaacau      480

ggcuagaaag uagagaguau acaaaacacc uaauaagagu cgaaaauugg auuuuuagaa      540

acccaggauu ugcauuggcg gcagcugcua uugcaugguu acuaggaagu ucaacuucac      600

aaaaaguuau auaucuagua augauacuau uaauagcacc ugcguauuca auuagaugua      660

uaggaguuuc gaauagagau uuuguagaag ggaugucagg uggaacaugg guagacguag      720

uacuagaaca cggaggaugu guaacaguca uggcacaaga uaaaccaaca guugacauag      780

aauuaguaac aacaacaguu aguaauaugg cagaagucag aucauauugc uaugaagcca      840

gcauaucuga uauggcaagc gacucaagau guccaacaca aggugaagcg uaucuugaua      900

aacaaucaga uacccaauau guauguaagc gaacauuagu agauagaggg uggggaaaug      960

gaugcggacu guuuggaaaa ggaagucuag ugacaugcgc aaaauucgca uguucaaaaa     1020

aaaugacagg aaaaagcaua caaccggaaa auuuagaaua uagaauaaug uuaaguguac     1080

augguucaca acauagugga augauaguca augauacugg acacgaaaca gaugaaaauc     1140

gagcaaaagu ugaaauaacc ccuaauucac caagagcaga agcuacauua ggagguuuug     1200

gaagucuagg auuagacugu gaaccaagaa cagggcuaga cuuuagcgau uuauauuauu     1260

uaacaaugaa uaauaaacau ugguugguac auaaagaaug guuucacgac aucccacuac     1320

cguggcaugc uggagccgac acugguacac cacauuggaa uaauaaggaa gcauuaguag     1380

aauuuaaaga ugcacacgca aaaaggcaaa caguuguagu cuugggcagu caggagggag     1440

caguucauac cgcucuagcu ggagcauuag aagcagaaau ggauggagca aaagguagau     1500

uaucaucugg acaccuaaaa uguagacuua aaauggacaa auugagacuu aaaggugucu     1560

cauauuccuu auguacagcu gccuuuacau uuacaaaaau cccagcugaa acauuacaug     1620

gaacagugac aguggaagua caauacgcag gaacagaugg accuuguaaa guuccagcac     1680

aaauggcagu ugauaugcaa acacuaacac caguuggcag auuaauuacg gccaacccag     1740

ugauaacaga aaguacagaa aauucaaaga ugauguuaga acuagauccu ccuuuuggag     1800

auucauauau ugucauagga guaggagaaa aaaaaauaac ucaucauugg cauagaucag     1860

gaucaacaau aggaaaagca uucgaagcaa caguuagagg agcuaaaagg auggccguac     1920

uaggagacac agcaugggau uuuggaucag ugggaggagc auuaaacuca uugggaaaag     1980

ggauacacca aauuuuuggc gcagcauuua aaucuuuauu ugggggcaug ucuugguuca     2040

gucaaauauu aauuggaaca cugcucaugu ggcuuggacu uaauacaaaa aacggaucaa     2100

uaagcuuaau gugucuugca uuaggaggag uucuaauauu uuuaaguacu gcaguaucug     2160

cagauguagg auguagugug gauuucagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7021
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 2 opt

<400>  7021
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc aagaaaggaa aaaaaaagaa gaggagcaga uacaagugua ggaauaguag      120

gcuuacuacu uaccacagca auggcugccg aaguaacaag aagaggauca gcauauuaca      180

uguauuuaga uagaaacgau gcaggagaag caaucucuuu uccaaccaca cucgggauga      240

acaaauguua cauacaaaua auggacuuag gacauaugug ugaugcaacu augaguuaug      300

aauguccuau gcuagaugag ggggucgaac cugaugaugu ugauuguugg uguaauacca      360

cuagcacaug gguaguguau gguacauguc aucacaaaaa aggugaagca agaagaucaa      420

gaagagcagu aacauuacca agucauagca ccagaaaauu acaaacaaga ucgcaaacau      480

gguuagaaag uagagaauau acuaaacauc uaauaagggu agaaaacugg auauuuagaa      540

auccaggcuu ugcauuagca gcggcugcaa ucgcaugguu acuagguagu ucaacaucac      600

aaaaaguuau auaucuaguu augauacuau uaauagcacc agcauacuca auaagaugca      660

uaggaguaag uaauagagau uucguagaag guaugagugg gggcacaugg guugacguug      720

uuuuagaaca ugguggaugu guuacuguaa uggcacaaga caaaccaacu guagauauag      780

aacuaguaac gacaacagua ucaaacaugg cugaagugag aucauauugu uaugaagcca      840

guauaaguga uauggucagu gauucaagau guccaacaca aggugaagca uauuuagaua      900

aacaaucaga cacucaauac guuuguaaaa ggacucucgu agacagagga uggggaaacg      960

gauguggauu auuugguaaa gggucuuuag uaacaugcgc aaaauuugcg uguagcaaga     1020

aaaugaccgg aaaaaguauu caaccugaaa auuuagagua uagaauuaug cuaaguguac     1080

acggaaguca acauucaggu augauaguua acgauacagg ucaugaaaca gaugaaaaua     1140

gagcuaaagu agaaauaaca ccaaauucac ccagggcaga agcaacauua gggggcuucg     1200

gaucauuagg acuagauugu gaaccucgaa cugggcucga uuuuagugau cuuuauuauu     1260

ugacaaugaa uaauaaacac uggcuugugc auaaagaaug guuucacgau auaccauuac     1320

cauggcacgc cggagcggau acaggaacuc cacauuggaa caacaaagaa gcauugguag     1380

aauuuaaaga cgcccaugcu aaaagacaaa cagucguagu acuaggaucu caagaaggug     1440

caguacauac agcgcuagca ggagcguuag aagcggaaau ggauggugcu aaaggaagau     1500

uaagcagcgg acaucugaaa ugcagauuaa aaauggacaa auuaagauua aaaggagucu     1560

cauauucauu auguacagcu gcauuuaccu ucacaaaaau accagcagag acuuugcaug     1620

guacaguaac aguagaagua caauaugcug gaacagacgg ucccuguaaa guaccugcac     1680

aaauggcggu agauaugcaa acacuaacuc ccguaggaag acuaauaaca gcuaauccgg     1740

uuauaaccga aaguacugaa aauagcaaaa ugauguuaga auuggaccca ccauuuggag     1800

auaguuauau uguuauaggu guaggcgaaa aaaaaauaac ucaccauugg cauagaagug     1860

guaguacaau aggcaaagca uucgaagcaa cuguaagagg agcaaaaaga auggcuguac     1920

uaggcgauac agcaugggau uucgguagcg uaggaggagc acuaaacucu uuagguaaag     1980

guauucacca aauauuuggu gcagcauuua agucccuauu cggaggaaug agcugguuua     2040

gucaaaucuu aauaggaacu cuacuaaugu gguuaggacu aaauacaaaa aaugguagua     2100

uaaguuuaau gugcuuagca cuuggaggag uauuaauuuu ucuaagcaca gcaguaucag     2160

cagacguugg augcucugua gauuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7022
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 2 opt

<400>  7022
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc ccgaaaagaa aagaaaagaa gaggagcaga uacaaaugua gguauagucg      120

gccuacuacu aacaacagca auggcagcag aaguuacacg ccguggauca gcuuauuaua      180

uguaccuaga uagaaaugau gcaggagaag cgauuaguuu uccgacaaca uuagguauga      240

auaaauguua cauacaaaua auggaccugg gacacaugug cgacgcuaca augagcuaug      300

aaugcccaau gcuagacgaa ggaguagaac cagaugacgu agacugcugg uguaauauga      360

caucaacaug ggucguauau ggaacaugcc aucauaagaa aggagaagca agaagaucuc      420

ggagagcggu aacucuccca agucauagua cuagaaaacu acaaacaaga ucucaaaccu      480

ggcuagaauc aagagaauau acuaaacauc uaauaagagu agaaaauugg auauuuagaa      540

acccugguuu cgcccuagca gcugcugcaa uagccuggcu acuaggaagu aguacaagcc      600

aaaaaguaau auauuuagua augauauuac ucauugcucc ggcguauagu auuagaugua      660

uuggaguaag uaauagagac uucguagaag gaaugucggg aggcacaugg guagauguag      720

uacuagaaca uggaggaugu guaacgguua uggcucaaga uaaaccaaca guagauauag      780

aauuagucac uacaacagua ucaaauaugg cagaaguaag aucuuacugu uaugaagcau      840

caauaaguga uauggcuagc gacucuagau gcccaaccca aggagaagca uacuuagaua      900

aacaaucaga uacacaauau guauguaaaa gaacauuagu agacagaggg uggggaaaug      960

gauguggauu auucggaaaa ggaucccuag uaacaugcgc aaaauuugcc uguucaaaga     1020

aaaugacagg aaaaucaaua caaccagaaa acuuagaaua uagaauaaug uuaaguguac     1080

acgguucaca acauucagga augauaguaa augauaccgg ucaugaaacu gaugaaaaua     1140

gagcaaaagu ggaaaucacc ccaaauucac caagggcaga agccacucua ggaggguuug     1200

guucacuagg acuugauugu gaaccaagaa caggacuaga uuuuucagau uuauauuauu     1260

uaaccaugaa uaauaaacau ugguuaguuc auaaagaaug guuucaugau auuccccuuc     1320

cauggcaugc aggagcagac acaggaacac cucauuggaa caacaaagaa gcucuaguag     1380

aauuuaaaga ugcacacgca aaaagacaaa cuguagucgu ucuaggauca caagaaggag     1440

caguacacac agcacuagca ggagcacucg aagcagaaau ggauggagca aaaggacgac     1500

uaagcagugg acauuuaaaa ugucgacuua aaauggauaa auuaagauua aaaggaguaa     1560

guuacucauu auguacagca gcauuuacau ucacaaaaau acccgcagaa acccuacaug     1620

gaacaguaac uguggaaguc caauacgcug gcacagaugg accauguaaa guaccagcuc     1680

aaauggcagu agacaugcaa acauuaacuc caguagguag auuaauaacu gcaaauccug     1740

ucauaacaga aucaacggaa aacuccaaaa ugauguuaga acuagaccca ccauuuggag     1800

acaguuauau aguuauaggu guaggugaaa agaaaauuac acaucauugg cauagaagug     1860

gaucaaccau aggaaaagcc uuugaagcaa caguaagagg agccaaaagg auggcaguac     1920

uuggagacac agcaugggac uuuggaagcg uaggaggagc auuaaacagc cugggaaaag     1980

gaauacauca aauauucgga gcagcauuua aaagccuuuu uggagguaug ucgugguucu     2040

cccaaauccu aauuggaacu cucuuaaugu ggcuaggacu uaauaccaaa aacggaucaa     2100

uaucauuaau gugucuagca uuaggaggug uacuaauauu ucuaaguaca gcaguaucag     2160

cagacguagg auguucagua gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7023
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 2 opt

<400>  7023
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc uaggaaagaa aaaaaaagaa gaggggcaga cacaagugua gggauagucg      120

gacuauuauu aacuacagca auggcagcag aaguuacaag gagagguucu gcauacuaua      180

uguauuuaga ucgaaaugac gcaggcgagg cuauaaguuu ucccacaacu cucggaauga      240

auaaauguua uauucaaaua auggaucuug gacacaugug ugaugcaacc augucauaug      300

aauguccaau guuagaugaa ggaguagagc cagacgaugu agacuguugg uguaacacua      360

caucaacuug gguaguuuac ggaacauguc aucauaaaaa aggagaagcg agaaggagua      420

gaagagcagu aacauuaccu ucacauucaa ccaggaaauu acaaacaaga agccaaaccu      480

gguuagaauc aagagaauau acaaagcauu uaauacgcgu ggaaaacugg auauuuagga      540

acccaggcuu cgcuuuagca gcggcugcga uagcauggcu ccucggauca ucaacgucac      600

aaaaaguaau auauuuagua augauuuuau ugauagcacc agcauauuca auacgaugua      660

uuggcguguc aaaucgagau uuuguugaag gaauguccgg aggaacuugg guagauguag      720

uauuagaaca uggaggaugu guaacaguaa uggcacaaga caaaccuaca guugauauag      780

aauuaguaac aacaaccgua ucaaauaugg cagagguaag aagcuauugu uaugaagcuu      840

caauaucaga uauggcuuca gauagcagau guccaacaca gggagaagcu uaucuagaca      900

aacaaucaga cacacaauau guauguaaaa ggacacuagu agauagaggu uggggaaaug      960

guuguggucu uuucgguaaa ggaagcuuag uaaccugcgc aaaauuugcu uguaguaaaa     1020

aaaugacugg aaaaagcauu caaccagaaa auuuagaaua uagaauaaug uuaaguguac     1080

acggcucuca gcauagcgga augauaguaa augauaccgg ccacgaaacu gacgaaaaua     1140

gagccaaagu cgaaauuacu ccuaauucuc caagggcuga agcugcauua ggugguuuug     1200

gaucgcuagg ccuugacugc gaaccaagaa caggauuaga cuucagugau cuauauuauu     1260

uaaccaugaa uaauaaacac ugguuagucc auaaagaaug guuccaugau auaccgcuac     1320

cauggcacgc aggugcagau acagguaccc cacacuggaa uaauaaggaa gcauuagucg     1380

aauuuaaaga ugcacacgcg aaaagacaaa caguaguagu auuaggauca caagaagggg     1440

cuguacacac agcacuagcu ggagcacuag aagcagaaau ggauggugcc aaaggcagau     1500

uaaguagugg acaucuuaaa uguagacuua aaauggauaa auuacgacuu aaagguguaa     1560

guuauucacu auguacagca gcauuuacuu uuacuaaaau uccagcagaa acacuucaug     1620

gcaccguaac uguugaagua caauacgcag gaacugaugg uccuuguaaa guuccagcac     1680

aaauggcagu ggauaugcaa acauuaaccc caguaggcag auugauaacu gcaaauccgg     1740

uaauuacaga aaguacagaa aacaguaaaa ugaugcuuga gcuugaucca ccauuugggg     1800

acucuuacau aguaauagga gucggugaaa aaaaaauaac acaccacugg cacagaucag     1860

guucaacuau aggaaaagcc uucgaagcua caguaagagg ugcaaaaaga auggcaguau     1920

uaggagauac agcaugggac uucggaucug uaggaggagc auuaaauuca uuaggaaaag     1980

gaauacauca aaucuucgga gccgcauuua aaagucuauu cgguggaaug ucuugguuua     2040

gccaaacauu aauaggcacu uuauuaaugu ggcuagguuu aaauacaaaa aaugguucaa     2100

uuaguuuaau guguuuagca uuaggaggug uacuaauauu cuugaguacc gcaguaucag     2160

ccgaugucgg uuguucugua gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7024
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 2 opt

<400>  7024
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uuauaaacgc acgaaaagaa aaaaaaagaa gaggggcaga aacaagcgua ggaauuguag      120

gucuacuauu aacaacagcu auggcugcug aaguaacacg aagaggauca gcauauuaca      180

uguauuuaga uagaaaugau gcgggagaag caaucucauu cccuacaacg uuagggauga      240

auaaauguua uauccaaaua auggaucuag gccacaugug cgaugcuaca auguccuaug      300

agugcccaau gcuagaugaa ggaguagaac cagaugaugu agauuguugg uguaauacaa      360

caaguacaug gguaguauau gguacauguc accacaaaaa aggagaagcg aggcggucaa      420

ggagagcagu uacauuacca ucacacagua caagaaaacu acagacacga ucacaaacau      480

gguuggaaag uagggaauac acuaaacauc uaauacgugu ugaaaauugg aucuuuagaa      540

acccaggguu cgcucuagca gcggcagcua uagcaugguu acuagguucu ucaacaucgc      600

aaaaaguaau auaccucgua augauucuac uaauagccuu agcuuacuca auaagaugca      660

uaggcguauc caauagagau uuuguagaag gaaugucagg aggaacuugg guagauguag      720

uuuuagaaca cggaggcugu guaacaguaa uggcacagga caaaccuaca guagauauag      780

aauuaguaac aacaacagua ucuaauaugg cagaaguucg aucauauugu uaugaagcau      840

cgauuucaga uauggcaagc gauaguagau guccuacgca aggugaagca uauuuagaua      900

aacaaucuga uacucaauau guaugcaaaa gaacauuagu agauaggggu uggggaaaug      960

gauguggacu auucggaaaa ggaagcuugg ucacuugugc aaaauuugca uguucaaaaa     1020

aaaugacagg aaaaucaaua caaccagaaa acuuagaaua cagaauaaug cugagcguac     1080

auggaagcca acauagcgga augauaguaa acgacacagg acaugaaaca gaugaaaaca     1140

gagcaaaagu agaaauaacu cccaauucac caagagcaga agcaacucuc ggaggauucg     1200

gaagcuuagg acuagacugu gaaccgagaa caggucuaga cuucucugau cucuacuauc     1260

uaacuaugaa uaacaaacau uggcuaguuc auaaagaaug guuccaugac auaccauuac     1320

cauggcaugc uggagccgau acaggaacac cacauuggaa caauaaagaa gcucuaguug     1380

aauuuaaaga ugcacaugca aaaagacaaa ccgucguugu auuaggauca caagagggag     1440

cuguccauac agcauuagca ggagcauuag aagccgaaau ggauggagca aaaggaagac     1500

uaucaucagg acauuugaaa uguagauuaa aaauggauaa auuaagacua aaagggguau     1560

cauacucacu auguaccgca gcauuuacau uuaccaaaau accagcagaa acauuacaug     1620

gaacaguuac aguggaaguc caauaugccg gaacugaugg uccaugcaag guacccgccc     1680

aaauggcagu agacaugcaa acacuaacac cagugggaag auuaauaacc gccaauccag     1740

uaauuacaga aucaacagaa aauucaaaaa ugaugcucga auuagauccg ccauucggag     1800

auagcuacau uguuauugga guaggagaaa aaaaaauuac acaccauugg caccgaagcg     1860

gaagcacuau uggaaaagca uuugaagcua cuguaagagg agcaaaacga auggcaguac     1920

uuggagauac ugcuugggau uuuggaagug uagguggcgc acuaaacagc uuagguaaag     1980

gaauacauca aauauucggc gccgcauuca aaagcuuguu ugggggaaug ucaugguucu     2040

cacaaauauu gauagguaca cuuuugaugu ggcuaggacu aaauacaaaa aacgguucaa     2100

uaagucuuau gugucuagcc cucgguggag uacuuauauu uuuaaguacc gcaguaagcg     2160

cagauguugg cuguagugua gacuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7025
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 2 opt

<400>  7025
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc gagaaaagaa aaaaaaagaa gaggagcuga uacaaguguu ggaauugugg      120

gucucuuauu aacaacagca auggcagcag aaguaacaag aagaggguca gcauacuaua      180

uguaucuaga uaggaaugau gccggcgaag cuauaucauu uccuacaaca uuaggaauga      240

auaaaugcua uauacaaaua auggaucuug gucauaugug ugaugcaacc augaguuaug      300

aauguccgau guuggacgaa ggaguugaac cagaugaugu agacugcugg uguaauacaa      360

caaguacaug ggucguauau ggcacauguc aucauaaaaa aggagaagcg agaagaucgc      420

guagagcugu aacacuacca ucacauagua ccagaaaacu acaaacgaga agucaaacau      480

ggcuagaauc aagagaauau acaaaacacu uaauacgagu agaaaauugg auauuuagaa      540

acccaggauu cgcauuagca gcagcggcaa uugcaugguu auuaggauca aguacgaguc      600

aaaaaguaau auauuuagua augauacuau uaauagcacc ugcauacuca auaagaugua      660

uaggaauaag uaaucgagau uucguggaag gaaugucagg agguacaugg guagauguag      720

uucuugaaca uggaggaugu guaacuguaa uggcccaaga caagccaaca guagauaucg      780

aauugguaac aacgacaguu aguaauaugg cagaaguacg aaguuauugu uacgaagcau      840

cuauuucuga uauggcauca gacaguagau guccaacaca aggagaggcu uaucuggaca      900

aacaaaguga cacacaauau guauguaaac guacgcuagu ugaucgagga uggggaaacg      960

gauguggguu auucggaaaa gggaguuuag uaacaugugc aaaauuugca uguucgaaaa     1020

agaugacggg aaaaagcaua caaccagaga auuuggaaua uagaauaaug cuaagugugc     1080

acggaucaca acacucaggu augauaguca augauacugg acaugaaacc gaugaaaauc     1140

gugcaaaagu agaaauuaca ccaaauaguc caagagcaga agcaacacua ggcggauucg     1200

ggauauuagg auuagauugu gaaccaagaa caggacuaga uuuuagcgau cuauacuacu     1260

uaaccaugaa uaacaaacau ugguuaguac auaaagagug guuccaugau aucccucuuc     1320

cauggcacgc uggugcagau acaggaacac cacauuggaa caacaaagaa gcacuaguag     1380

aauuuaaaga ugcacacgca aaaagacaaa caguaguagu auuaggaagc caagagggag     1440

caguacauac agcacuagca ggugcauuag aagcagaaau ggacggagcg aaagguagau     1500

ugaguagugg acauuuaaaa ugcagauuaa aaauggacaa acuaagacuu aaaggaguaa     1560

guuacagucu uuguacagca gcauuuacuu ucacaaaaau accagcugaa acacuucaug     1620

gaacaguuac uguagaaguu caauaugcag guacagaugg accauguaaa guacccgcac     1680

aaauggcagu agauaugcaa acccugacac caguaggcag acuaauaaca gcgaacccag     1740

uaauaacaga aagcaccgaa aacucaaaaa ugaugcuaga auuagaccca ccauuuggag     1800

acaguuauau aguuauaggu guuggagaaa aaaaaauaac acaucacugg cacagaagug     1860

guaguacaau aggcaaagca uuugaagcua cugucagagg cgcaaaaaga auggccguau     1920

uaggagauac agcaugggac uuugggagcg uuggaggugc auugaauagc cucgguaaag     1980

ggauccauca gauauuugga gcagcauuua aaagcuuauu cggaggaaug ucaugguucu     2040

cacaaauauu aauugguaca uuauuaaugu gguuagguuu gaacacaaag aaugguucaa     2100

uuucauuaau gugcuuagcc uuaggaggag uauuaauauu ucuuaguaca gcaguaucgg     2160

cugauguugg auguuccgua gauuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7026
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 2 opt

<400>  7026
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa aagaaaagaa gaggagcaga uacaagugua ggaauagugg      120

gucuauuauu aacgacugca auggcagccg aagugacaag acgaggaagc gcauauuaua      180

uguaucuaga uaggaaugau gcuggagaag caauuucauu uccaaccacu uuaggaauga      240

auaaaugcua uauucaaaua auggaucuag gacauacaug ugaugcaaca augaguuaug      300

aauguccaau guuagaugaa ggcguagaac cagaugacgu agacuguugg uguaauacua      360

caaguacuug gguagucuau ggaacaugcc aucauaagaa aggagaagcu cgacggucac      420

guagagccgu aaccuuaccu ucucacucaa uaagaaaauu acaaacacga agucaaacau      480

ggcuagaaag cagagaauau acaaaacauc uaauacgagu cgaaaauugg auauuucgaa      540

auccgggauu ugccuuagcc gcggcagcaa uagcgugguu acucgguagu ucaacaucac      600

aaaaaguaau uuacuuggua augauacugc uuauagcccc agccuacuca auaagaugua      660

uaggaguaag uaauagagau uuuguugaag gaaugucagg cggaacuugg gucgauguag      720

uauuagaaca cggcgguugu gugacaguua uggcccaaga caaaccaaca guagauauag      780

aauuaguaac aacuacagua uccaauaugg cagaaguaag aaguuauugu uaugaagcau      840

caauaucgga uauggcaucc gauaguagau gucccacaca aggagaagca uaucuagaua      900

aacaaucaga cacacaauau guauguaaac gcaccuuagu agacagaggu uggggaaacg      960

gauguggauu auuuggaaaa ggaagucucg ugacuugcgc uaaauuugca uguagcaaga     1020

aaaugacagg caaaaguaua cagccagaaa auuuagaaua uagaauaaug uuaaguguac     1080

auggaaguca acauagugga augauuguaa augauacagg acaugaaaca gaugaaaaca     1140

gagcaaaagu cgaaauaaca ccaaacucac cacgagcaga agccacacua ggaggauuug     1200

gaagccuagg cuuagauugu gaaccaagaa caggacuaga cuuuucagau uuguacuauu     1260

uaacuaugaa uaauaaacac uggcuaguuc auaaagaaug guuccaugac auaccauuac     1320

cauggcacgc uggugcagau acagggacac cacacuggaa uaauaaagag gcuuugguag     1380

aauuuaaaga ugcucacgcc aaaagacaaa caguaguagu auuaggauca caagaaggag     1440

cuguacauac agcauuagcu ggugcguuag aagcagaaau ggauggagca aaaggucgau     1500

uaagcucagg acacuuaaaa uguagauuaa aaauggacaa auuaagacua aaagguguau     1560

cauauagucu augcacagcu gcauucacau ucacaaaaau acccgccgaa acacuucaug     1620

guacagucac agucgaaguc caauaugcag gaacugaugg accaugcaaa guaccugcgc     1680

aaauggcugu agacaugcaa acgcuaacuc caguaggaag auuaauaaca gcaaauccag     1740

ucauuacuga aucaacugaa aauaguaaaa ugaugcuaga auuagaucca ccauuuggug     1800

auuccuauau aguaauaggc guaggugaaa agaaaauaac ucaucauugg cacagaagug     1860

ggucaacuau aggaaaggcu uucgaagcca caguaagagg ugcaaaaaga auggcaguau     1920

uaggagacac agcaugggac uuuggaagug uaggaggagc auuaaacagu cuagguaaag     1980

gaauacauca aauauuugga gcagcauuca aaucauuauu cggcggaaug agcugguuuu     2040

cacaaauauu aauaggaacc cucuuaaugu gguuaggacu aaaugcuaaa aauggaucaa     2100

uuucacuuau gugucucgca cuaggaggag uccuaauauu uuugucgaca gcaguaucag     2160

cagauguagg uuguucugug gacuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7027
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 2 opt

<400>  7027
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaaugc uagaaaagaa aaaaaaagac gcggggcaga uacaaguguu ggaauuguag      120

gauuguuacu aacaacagca auggcagcag aaguaacaag acgagggagu gcauauuaca      180

uguaucuaga uaggaaugau gcaggggaag cuauaagcuu uccaacaacu cuagguauga      240

auaaaugcua uauacaaaua auggaccugg gucauaugug ugaugcaaca augagcuaug      300

aauguccaau guuagaugaa ggaguagaac cagaugacgu agacuguugg ugcaauacaa      360

caaguacaug gguaguauau ggaacauguc aucauaaaaa aggagaagca caacgaagca      420

gaagagcugu aacacuacca agccacucua cuagaaaauu acaaaccaga ucacaaacgu      480

ggcuggaauc acgugaauau acaaaacauc uuauuagagu agaaaauugg auauucagaa      540

auccgggguu ugcauuagca gcagcugcaa uagcaugguu acuagguagu ucuacuuccc      600

aaaaagucau auacuuaguu augauauuau uaauagcacc agcuuacagc auacgaugua      660

uaggcguuag uaauagggau uuuguagaag guaugucagg aggcacaugg guagauguag      720

uuuuagaaca cggcggaugu gucacuguaa uggcgcaaga caagccaaca guagacauag      780

agcuuguuac aacaacggua ucaaauaugg cagaagugag gucauauugu uacgaggcau      840

caauaaguga uauggcauca gauucaagau guccaacaca aggagaggcc uauuuagaua      900

aacaaagcga uacacaauac guaugcaaaa gaacacuggu ugauagagga uggggaaaug      960

gauguggacu guuuggaaaa ggaaguuuag ugacaugcgc gaaauuugcc uguuccaaaa     1020

aaaugacagg aaaaagcaua caaccagaga auuuagaaua uagaauaaug uuaagugugc     1080

acggaaguca acacagugga augauuguaa augacacagg ucacgaaaca gaugaaaaua     1140

gagcuaaagu ggaaauaacg ccuaauucac caagggcaga agcaacauua ggaggauucg     1200

gaaguuuagg gcuagacugu gaaccaagga caggguuaga uuuuagcgau cuguauuauc     1260

ugacgaugaa caacaaacau ugguuaguac auaaagaaug guuccacgau auaccauuac     1320

cguggcaugc cggagcagac accggcacac cacauuggaa uaauaaagaa gcauuaguag     1380

aauuuaaaga cgcacaugca aaacgucaaa caguaguagu ucuaggaagu caagaaggug     1440

caguacacac ugcauuagcc ggagcacuag aggcggaaau ggacggagca aaaggucgac     1500

uuucaagugg ucaucuaaaa uguagauuaa aaauggacaa acuucgacua aaaggaguau     1560

cuuauaguuu augcacagca gcauucacau uuacgaaaau uccagcagaa acauuacaug     1620

gaacagugac uguagaggua caguacgcgg gaacagaugg accauguaaa gugccagcac     1680

aaauggcagu ugacaugcaa acauugacac caguaggacg acucaucaca gcaaaucccg     1740

uaauaaccga aucaacggaa aauucaaaaa ugauguugga auuagaccca ccauuugggg     1800

acaguuauau uguaauagga guuggagaaa aaaaaauuac ucaucacugg cacaggagcg     1860

gaucaacaau agguaaagcg uucgaagcaa cuguucgagg agccaaacga auggcaguau     1920

uaggagauac agcaugggau uuugguucag uaggaggagc auuaaacagu uuaggaaaag     1980

gaauacacca aauauuugga gcggcauuua agucauuguu uggagggaug ucaugguucu     2040

cacaaauacu aauaggaaca cuguuaaugu gguuaggauu aaauaccaaa aacggcagua     2100

uuaguuuaau gugcuuagca cuaggaggag uguuaauauu cuuaaguacu gcuguaagug     2160

ccgauguagg cuguaguguc gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7028
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 2 opt

<400>  7028
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc aagaaaagaa aaaaagagaa gaggagcaga uaccagugua gguauuguag      120

gucuguuauu gacaacagcu auggcagcag aaguaacaag aagaggaagu gcuuauuaca      180

uguauuuaga uagaaaugau gcaggagaag caauaucauu cccaacaaca uuaggaauga      240

auaaaugcua uauacagaua auggacuuag gacacaugug ugaugcaacu augucguaug      300

aauguccaau gcuagaugaa ggaguagaac cggaugaugu ugauugcugg uguaauacaa      360

cgucaacaug gguaguuuau ggaaccuguc aucauaagaa aggggaagcu agacguucaa      420

gaagagcagu aacccuacca ucacacagca cgcgaaaauu acaagcaaga ucacagacuu      480

gguuagaauc acgagaauau acgaaacacu uaauacgagu agagaauugg auauuuagaa      540

auccaggauu ugcauuagca gcagccgcua uagcaugguu acuuggauca ucaacauccc      600

aaaaaguaau cuaccuagua augauacuau uaauagcacc agcuuauagu auuagaugua      660

uaggaguaag uaauagagau uuuguugaag gaaugagugg uggaacgugg guagauguag      720

uauuagaaca uggcggaugu guaaccguua uggcacaaga caaaccaaca guggacauag      780

aacuaguuac aacaacugua agcaacaugg cggaaguaag gucauauugu uacgaagcua      840

gcauaaguga cauggcauca gauaguagau guccaacaca aggcgaagcc uaucuagaua      900

aacaaucaga cacacaauau guauguaaga gaacacuugu agaucgugga uggggaaacg      960

guuguggauu guuugguaaa ggaucacuag uuacuugugc aaaauucgca uguaguaaaa     1020

aaaugacggg aaaaagcaua caaccagaaa auuuagagua cagaauaaug cugaguguuc     1080

augguucaca acauucagga augauaguaa acgauacagg ucacgaaacu gaugaaaaca     1140

gagcgaaagu agaaauaacu ccaaauaguc cuagagccga agcaacuuua ggcgguuuug     1200

guucauuagg auuagacugu gaaccaagaa cuggauuaga uuucucugau uuauacuacu     1260

uaacuaugaa uaauaaacau uggcuagucc auaaagaaug guuucaugac auaccauuac     1320

ccuggcacgc aggugccgac acagguacac cacauuggaa uaauaaagaa gcauuaguug     1380

aauuuaaaga ugcacaugca aaaagacaaa caguaguagu auuagggagu caagaaggag     1440

caguacauac agcuuuagca ggggcauugg aagcagaaau ggauggcgcu aaagguagac     1500

uaucaucugg acacuuaaaa ugucgacuaa aaauggauaa auuaagacuu aaaggaguaa     1560

guuauagccu augcacggca gcguuuacau uuacaaaaau accugcugaa acuuuacaug     1620

gcacaguuac aguagaagua caguaugcgg gaacagaugg ucccuguaaa guaccagcuc     1680

aaauggcugu agauaugcaa acguuaacac caguaggcag auuaauaaca gcaaacccag     1740

uuauaacaga aaguaccgaa aauagcaaaa ugauguugga auuagaucca ccuuuuggag     1800

auucauauau aguaauagga guuggagaaa aaaaaauaac acaucauugg cauagaagug     1860

gaucaaccau aggcaaagcu uuugaggcaa caguaagggg agcaaaacga auggcaguau     1920

uaggagauac agcaugggau uuugguucag uaggaggagc acucaauagu cuuggcaaag     1980

gcauacauca gauauuugga gccgcuuuca aaucauuauu uggugggaug ucaugguucu     2040

cacaaauucu gauaggaaca cuguuaaugu gguuaggccu uaauacgaaa aauggaucaa     2100

uuagcuuaau gugcuuagca uuaggaggag uacuaauuuu uuuaucaacc gcaguaagcg     2160

cugauguagg gugcagugua gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7029
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 2 opt

<400>  7029
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc acgaaaagag aagaaaagac ggggcgcaga cacaaguguc ggaauuguag      120

gauuacuucu aacaacagca auggcagcag aaguaacaag aagaggaagu gcauauuaua      180

uguaucugga uagaagcgau gcaggagaag caauuagcuu cccaacaacg uuagguauga      240

auaaaugcua cauacaaaua auggauuuag gacauaugug cgacgcaaca augaguuacg      300

aauguccaau gcuggaugaa ggaguagaac cagacgaugu ggauuguugg ugcaacacua      360

caaguacuug ggugguauau ggaacuugcc aucacaagaa aggugaagca agaagaucaa      420

ggagagcugu uacguuacca ucacacagua caagaaaauu acaaacaaga agucaaacau      480

ggcuggaaag uagagaauac acaaaacacu uaauaagagu ugaaaauugg aucuuuagga      540

acccaggauu ugcacuagcu gcagcagcaa uagccuggcu acuagguagu aguacaagcc      600

aaaaaguaau auauuuaguc augauccuau uaauagcacc agcauacucu auaagaugua      660

uaggaguaag caauagagau uucguagaag gaaugucugg aggaaccugg guagauguag      720

uuuuagaaca cggaggcugc guaacaguua uggcccaaga uaaaccgaca guugauauag      780

aacuuguaac aacaacggua aguaauaugg cagaaguaag auccuacugu uacgaagcaa      840

guauaaguga cauggcuuca gauucaagau guccaacaca aggggaagcg uaccuugaua      900

aacaaucuga uacacaauau guuuguaaaa gaacccuagu agauagaggu uggggaaaug      960

gauguggacu uuuuggaaaa gggaguuuag uuacaugugc aaaauucgca uguaguaaga     1020

aaaugacagg uaaaaguaua caaccagaaa auuuggaaua caggauaaug uuaagugucc     1080

auggaaguca acauagcgga augauaguca augauacggg gcaugaaaca gacgaaaaca     1140

gagcaaaagu agaaauaaca ccuaauuccc cgcgagcaga agcaacauua gguggauuug     1200

gcucauuagg auuagauugc gaaccaagga cuggauuaga uuuuagugau uuguauuauu     1260

uaacaaugaa caauaaacau uggcuuguuc auaaagaaug guuucaugau auuccacuac     1320

cauggcacgc aggagcugau acaggcaccc cacauuggaa uaauaaagaa gcauuggucg     1380

aauuuaaaga ugcucaugca aaaagacaaa cgguaguagu auuaggauca caagaaggag     1440

caguacauac agcacuugcu ggugcuuuag aagcagaaau ggauggagca aaaggucgau     1500

uaaguagugg acauuuaaaa uguagauuaa aaauggacaa auugagacua aaaggugucu     1560

ccuauagcuu augcacagca gcauuuacau uuacaaaaau cccagcagaa acauuacaug     1620

gaacuguaac uguugaagua caauaugcag guacagaugg cccguguaaa guuccagcuc     1680

agauggcugu ugauaugcaa acauuaacac cugucggaag auuaauaacg gcaaaucccg     1740

uaaucacgga aaguacugaa aauaguaaaa ugaugcuaga auuagaucca ccauuugggg     1800

acagcuauau aguaauagga guaggugaaa agaaaaucac acaucauugg cauaggagug     1860

gaagcacaau aggaaaagcc uuugaagcaa caguaagagg agcaaaacga auggccgugc     1920

uaggugauac agcaugggau uucggaucag uggguggagc ucucaauucc uuaggaaaag     1980

ggauacauca aauauuugga gcugcauuua aaagucuauu uggggguaug ucuugguuua     2040

gucaaauauu aauuggaaca uuacuuaugu ggcugggauu aaacacuaaa aaugguucga     2100

uaaguuuaau guguuuagca uuaggugggg uuuuaauuuu ucuaaguaca gcaguauccg     2160

ccgauguggg uuguagugua gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7030
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 2 opt

<400>  7030
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa aagaaaagaa gagguacaga caccagugua ggaauaguag      120

gauuacuauu gacaacagca auggcugcag aaguuacacg cagaggaucu gcuuacuaua      180

uguaccuaga uagaagugau gcaggagaag ccauuucauu cccaacaacu uuagguauga      240

auaaauguua uauacaaaua auggaucuag gacacaugug cgacgcaacu augaguuaug      300

aaugcccuau gcuagaugaa ggaguagaac ccgaugaugu ugauuguugg uguaacacaa      360

caaguacaug gguaguauac ggaacauguc aucauaagaa aggcgaagca agaagaagca      420

gacgagcagu uacauuaccg agccauucaa caagaaaacu acaaacaaga ucucaaaccu      480

gguuagaaag ucgagaauau acaaaacauu uaauuagagu agaaaacugg auauucagaa      540

auccaggauu ugcuuuagca gcugcagcua uagcauggcu acuaggauca uccacaucac      600

aaaaaguuau auacuuagua augauucuau uaauagcccc agcauauuca auaagaugca      660

uagguguaag uaauagagau uucgucgaag gaaugagugg aggaacaugg guugacguag      720

uacuagaaca uggaggcugu guaacaguaa uggcccaaga uaaaccgacu guagauauag      780

aauuaguaac aacaacagua aguaauaugg ccgaagucag aucauacugu uaugaagcca      840

gcauaaguga uauggcaagc gauuccagau guccaacuca aggugaggca uauuuagaua      900

aacaaucgga uacacaauau gugugcaaaa gaacucuagu agacagagga uggggaaacg      960

gcuguggauu auuuggaaaa ggaagucuag uaacaugugc caaauuugca ugcucaaaga     1020

aaaugacugg aaaaucaaua caaccagaaa aucuagaaua ucgaauaaug uuaagugugc     1080

auggaaguca acacagcgga augauaguaa acgauacugg ucaugagaca gacgaaaaca     1140

gagcaaaagu agaaauaaca ccuaauucac cacgagcaga agcaaccuua ggaggcuucg     1200

gaucccuagg auuagauugu gaaccacgaa caggcuuaga cuuuagugau uuauauuauu     1260

uaacaaugaa uaacaaacau uggcugguac auaaagaaug guuucacgau aucccauuac     1320

cauggcacac aggugcagau acaggaacac cacauuggaa caacaaagag gcgcuuguag     1380

aauuuaaaga ugcucacgca aaaagacaaa caguuguagu auugggaucu caagaaggcg     1440

caguacauac agcauuagcu ggagcucuag aagcagaaau ggacggugca aaaggccgac     1500

ugucuucugg ucacuuaaaa ugcagacuca aaauggauaa auuaagacuu aaaggcguau     1560

cauacaguuu auguaccgcu gcuuucacau uuacaaaaau accagcugaa acuuuacaug     1620

gaaccguaac aguagaagug caauacgcug guacugaugg accauguaaa gugcccgcuc     1680

aaauggcagu agauaugcag acacuaacac cagugggacg auuaauuacu gcaaauccag     1740

uuauuacaga aggaacagaa aacucaaaaa ugauguuaga auuagaucca ccauuuggug     1800

auucauauau aguaauagga guaggugaga aaaagauuac acaucauugg cauagaucag     1860

gaucaacaau agggaaagcg uucgaagcua caguaagagg agcuaaaaga auggcaguau     1920

uaggagacac ggcaugggau uuuggaucag uggguggagu acucaauuca uugggaaaag     1980

gaauacacca aauauucgga gcagccuuca aaagccuauu uggaggaaug aguugguuua     2040

gucaaauacu cauaggaaca uuguuaaugu ggcuaggcuu aaauacuaaa aauggcagua     2100

uaucacuaau gugucuugca cugggaggag uacuuauauu ucuaucaaca gcuguauccg     2160

cagauguagg auguagcgua gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7031
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 2 opt

<400>  7031
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc aaggaaagaa aaaaaaagaa ggggagcuga uacaucagua ggaauaguug      120

gucuucuacu aacuacagca auggcagcgg aaguaacgcg aagaggauca gcauauuaua      180

uguaucuaga uagaaaugau gcaggagagg caaucucuuu uccuacaaca uuaggaauga      240

acaaauguua cauacaaaua auggacuugg gacauaugug cgaugcaacc augucauaug      300

aauguccuau guuggaugaa gguguagaac cugaugacgu ugauuguugg ugcaauacaa      360

caucaacaug gguaguauac gggacauguc aucacaaaaa aggugaagcu agaagaucaa      420

gaagagcugu uacauuaccu ucacacucaa cacgaaaauu acaaacaaga ucacaaacau      480

gguuagaauc aagagaauau acaaaacacc uaauaagagu agaaaauugg auauuuagaa      540

auccagguuu ugcauuagca gcagcagcaa uagcaugguu auuaggaucc ucaacgaguc      600

aaaaaguuau uuauuuagua augauuuugu uaauugcucc agcuuauuca auaagaugca      660

uagguguaag uaaucgagac uuuguagaag ggaugagugg ugggacaugg guagauguag      720

uauuagaaca cggcggaugc guaacggcaa uggcgcaaga caaaccaaca guagauauag      780

aacuaguuac aacuacgguu ucuaacaugg cagaaguaag aucuuauugc uacgaagcau      840

caauaucaga cauggcaucu gauaguagau guccaacaca aggggaagca uaucuagaua      900

aacaaucaga uacacaauau guauguaaac gaacucuagu agaccguggc ugggguaacg      960

gaugcgggcu cuuuggaaaa ggaucauuag uaacaugugc aaaauuugcu uguuccaaga     1020

aaaugacagg aaaaagcaua caaccagaaa accuugaaua uaggauaaug uuaucaguac     1080

auggcucaca acauaguggu augcuuguaa augauacagg acaugaaacg gacgaaaacc     1140

gagcaaaagu ugaaauaacu ccaaauaguc cgcgagcuga agcaacguua ggcggauuug     1200

gaagcuuagg gcuagauugu gaaccaagaa caggauugga uuucucagau cuuuauuauu     1260

uaacaaugaa uaauaaacau ugguuagcac acaaggaaug guuccaugau auaccccuac     1320

cauggcaugc aggugcagcc acgggaacuc cucauuggaa caauaaagaa gcauuaguag     1380

aauuuaaaga cgcacacgcc aaaagacaaa ccgucguugu acuuggauca caagagggag     1440

caguacauac agcauuagca ggagcauugg aagcagaaau ggauggugcu aaaggaagau     1500

uaucuagcgg acaucuaaaa ugcaggcuaa aaauggacaa acuacgauua aaagguguaa     1560

gcuauagucu augcacagca gcuuuuacau uuacaaaaau acccgcagag acuguugacg     1620

guacaguaac aguagaaggu caauauggag ggacugaugg uccuugcaaa guuccagcac     1680

aaauggcagu cgacaugcaa acacuaacgc caguugguag acuaauaaca gcgaaucccg     1740

ucauuacaga aucaacagaa aauucuaaaa ugauguuaga auuagaucca ccauuuggcg     1800

auagcuauau aguuaucgga guaggcgaaa aaaaaauaac ucaucauugg caccggagug     1860

gaagcacuau aggaaaagca uucgaagcca caguaagggg ugcaaaaaga auggcagugc     1920

uaggagauac ugcaugggau uuuggaagug uagggggcgc auuaaauagu uuaggaaaag     1980

gaauacauca aauaauaggu gcagccuuua aaagucuauu cggaggaaug aguugguuua     2040

gccaaauauu aauaggaacc uuacugaugu gguuaggacu uaacacgaaa aauggaagca     2100

uaucacuuau gugucucgcc uugggaggag uauuaauauu ucuaucgacg gcaguaagcg     2160

gcgggguagg uuguucagua gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7032
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 2 opt

<400>  7032
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc aagaaaagaa aaaaaaagga gagguacaga uacauccguc ggaauagugg      120

guuuauuacu uacaacugca auggcggcag aaguaacacg aagaggaagc gcauauuaua      180

uguauuuaga ucgaagugau gcgggagaag caauaagcuu cccaacaaca uuaggaauga      240

acaaauguua uauacaaaua auggauuuag gacacaugug ugaugcaacg augucauaug      300

aauguccaau guuagaugaa ggaguagaac cggaugaugu cgauuguugg uguaauacaa      360

caucaacaug gguaguauau ggaaccugcc aucauaaaaa aggugaagca agaagaucua      420

ggagagcagu aacgcuucca agucauagua caaggaaauu acaaacuaga agucaaacau      480

gguuagaaag uagagaauac acuaaacauu uaauacgagu agaaaacugg auauuuagaa      540

auccaggcuu cgcauuagcc gcagcugcca uagcaugguu auuagguucg aguacgucac      600

aaaaaguuau auauuuaguu augauucuau uaauugcccc cgcauauagu auaagaugca      660

uaggagugag uaaccgggau uuuguugaag gaaugucagg aggaacaugg gucgauguug      720

uauuagaaca ugguggaugc guaacaguua uggcacaaga caaaccaaca guggacauag      780

aauuaguaac aacaacggua aguaauaugg cagaaguacg aaguuacugu uaugaagcaa      840

guauaucaga uauggcaagc gauucuagau guccaacuca aggcgaagca uaucuagaua      900

aacaauccga uacacaauac gugugcaaga ggacguuggu agauagagga ugggguaaug      960

ggugcggguu auucggaaaa gguucauuag uaaccugcgc uaaauucgcg uguucuaaaa     1020

aaaugacagg aaaaucaaua caaccggaaa auuuagagua uagaauaaug uugucaguuc     1080

auggaaguca acauucagga augauaguaa augauacugg acaugaaaca gacgagaacc     1140

gagcaaaagu ugaaauaaca ccaaauaguc caagggcaga agcuacauua ggaggauuug     1200

gcucucuagg acuggauugu gaaccaagaa cuggcuuaga uuuuagcgac cuauauuacc     1260

uaacgaugaa uaauaagcac uggcuaguac acaaagaaug guuccacgau auaccauuac     1320

cauggcaugc aggugcagau acaggaacuc cacauuggaa uaauaaagaa gcacuagugg     1380

aguuuaaaga cgcacaugca aaaaggcaaa caguuguagu uuuaggcagu caagaaggcg     1440

cuguacauac agcauuagca ggagcacuag aagcggaaau ggauggagca aaaggaagau     1500

uaucaagcgg acaucuaaaa uguagguuaa aaauggauaa gcuuagauua aaaggaguca     1560

guuacucauu auguacggca gcuuuuaccu uuagcaaaau accagcagaa acacuacacg     1620

gcacaguuac ugaagaagug caauaugcug gaacugaugg cccaugcaaa guaccggcac     1680

aaauggcagu agauaugcaa acacuaacac caguaggaag auuaauaaca gcaaacccag     1740

uaauaacaga aucaacagaa aacaguaaaa ugauguuaga auuagaccca ccauuuggag     1800

auucauauau agucauagga guaggagaaa aaaaaauuac ccaucauugg cauagaagug     1860

guaguacaau aggcaaagca uucgaagcua caguaagagg agcaaaaagg auggcagucu     1920

uaggagacac agcaugggau uuuggaucag uaggaggagc uuuaaauagu uuaggaaaag     1980

gaauucauca gauauucgga gcagcauuua aaagccuauu cgguggaaug agcugguuuu     2040

cgcaaauauu gauaggaaca uuacuaaugu gguuaggauu aaacacaaaa aacggaucua     2100

uaagucugau gugccuagcg uuagggggcg uucuaauauu ccucucaaca gcaguaucag     2160

cggacguagg auguucagua gauuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7033
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 2 opt

<400>  7033
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc acgaaaagaa aaaaaaagaa gaggaacaga cacuucagua ggcauuguag      120

gacuacuacu uacaacagca auggcagcag agguaacacg aaggggaagu gcauauuaua      180

uguauuuaga cagaucagau gcaggagaag cgaucucauu uccuacaaca uugggaauga      240

auaaauguua cauccaaaua auggaucugg gccauaugug ugaugcaaca augaguuacg      300

aauguccaau guuagaugaa ggggucgagc cagaugaugu agauuguugg uguaauacca      360

cuucaacaug gguuguuuau ggaacaugcc aucauaaaaa aggagaagca agaagaucca      420

gaagggcagu aacauuacca ucacauucua cacgaaaauu acaaacccga ucgcaaacau      480

ggcuagaaag uagagaauac accaaacauu uaauaagagu cgaaaauugg auauucagaa      540

acccaggcuu ugcuuuagca gcagcugcga uagccuggcu acuaggaagu agcacuucac      600

aaaaagucau cuauuuagua augauuuuac uaaucgcucc agcauacagc auuagaugua      660

uuggaguuag uaauagagac uuuguagaag gaaugucagg cggaacaugg guugaugugg      720

uccuagaaca uggcggaugu guaacaguaa uggcccaaga uaaacccaca guagauauag      780

aauuaguaac gacaacagug agcaauaugg cagaaguaag aucauauugc uacgaagcuu      840

caauaucuga uauggcaagc gauucgagau gcccaacaca aggagaagca uaucucgaua      900

aacaaagcga cacucaauau guaugcaaaa gaacacuagu agacagaggc ugggguaaug      960

guugugguuu guuuggaaaa gggaguuuag uaacaugugc uaaauucgca ugcucgaaga     1020

aaaugacagg caaaucaaua caaccugaaa auuuagaaua cagaauaaug cuaaguguac     1080

augguucaca acauucaggu augauuguaa augauacagg ucacgaaaca gaugaaaaua     1140

gagcuaaagu agaaauaacc cccaauucac caagagcuga agccacacua ggaggcuucg     1200

guaguuuagg guuagauugu gaaccuagaa cagggcuuga uuuuucagac cuuuauuauc     1260

ucacaaugaa uaauaaacac uggcucguac auaaagaaug guuccaugac auaccucuac     1320

ccuggcacgc uggagcagau acaggaacgc cacacuggaa uaauaaagaa gcuuuaguag     1380

aauucaaaga ugcccacgcc aaaagacaaa cuguaguagu acuaggaagu caagaaggag     1440

caguacacac agcuuuagca ggagcauuag aagcggaaau ggacggagcg aaaggaagau     1500

uaucaucugg ucauuuaaaa uguagauuga aaauggauaa acuaagauua aaagguguuu     1560

cauauagcuu augcacagcu gcauuuacau uuacaaaaau accagcagaa acuuuacaug     1620

gcacaguaac aguagaaguc caguaugcag guacugaugg accuuguaag guacccgcac     1680

aaauggcagu agauaugcaa acacucacac caguaggaag acuaauuaca gcuaauccag     1740

uuauuacaga aagcacagaa aauuccaaaa ugaugcuaga guuagaucca ccuuuuggug     1800

acaguuauau agugauaggg gucggagaaa aaaaaauaac acaucacugg cauagaagcg     1860

gaucaacaau aggaaaagca uuugaagcca caguaagagg agcuaaaaga auggcuguac     1920

uaggcgauac agcaugggau uucggaagug uuggcggagc ucugaacagc cucgggaaag     1980

guauacauca aauauuuggu gcggcauuua aaaguuuauu uggaggaaug ucaugguuuu     2040

cacaaauauu aauaggaaca cuacuaaugu gguuaggucu caauacuaaa aauggaagua     2100

uaagcuuaau guguuuagca uugggaggag ucuuaauauu ccuaucaaca gcaguaagug     2160

cagauguagg auguaguguu gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7034
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 2 opt

<400>  7034
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauuaaugc acguaaagaa aagaaaagaa ggggaacaga uacgucugua ggaauaguug      120

gacuacuauu aacaacugca auggcuguag aaguaacaag aagaggaaau gccuauuaua      180

uguauuuaga uaggucggac gcaggagaag caauuucauu cccaacaaca augggaauga      240

acaaauguua uauccaaauc auggaccuug gacauaugug ugacgcaaca augucauaug      300

aauguccaau guuagaugaa gggguugaac cagaugaugu agauuguugg ugcaacacaa      360

caagcacaug gguagucuac ggaacauguc aucauaagaa aggagaagca cgaagaucaa      420

gacgagcagu uacauuacca agucacucaa caagaaaauu acaaacaaga ucacaaacau      480

ggcucgaaag uagagaauac accaaacauc ugauccgagu agaaaacugg auauuuagaa      540

aucccgguuu ugcucuugcu gccgccgcaa ucgcaugguu acuaggaucg ucuacuucac      600

aaaaaguuau auauuuagua augauauuau ugauagcucc agcauauucc auuagaugca      660

uaggaguuuc caauagagau uuuguagaag ggaugagcgg agguaccugg guagauguag      720

uauuagaaca cggaggaugc guuacaguaa uggcacaaga caaaccaaca guagacauag      780

aauuaguuac aacaacagua ucaaacaugg cagaaguucg uuccuauugc uacgaagcaa      840

guauuucaga uauggcaagu gauaguagau guccgacaca aggagaagca uauuuagaca      900

aacaaucaga cacucaauau guauguaaaa gaacucuagu agauagaggu uggggaaaug      960

gcuguggauu auucggcaaa ggaucucuag uaacaugugc aaaauuugcc uguaguaaga     1020

aaaugacagg aaaauccauc caaccagaaa auuuagaaua ucgaauaaug uuaucaguac     1080

auggaucaca acauuccgga augaucguca acgauacugg ucaugagacu gaugaaaaua     1140

gagcaaaagu agaaauaaca ccaaauucac cucgagcuga agcaacauug ggaggauuug     1200

gaucacuagg acuagacugu gaaccaagaa caggacuuga uuuuagcgac uuauauuacc     1260

uuacgaugaa caauaaacau ugguuaguac auaaagaaug guuucacgac auaccacuac     1320

cuuggcaugc aggagcagau acgggaacuc cccauuggaa caauaaagag gcuuuaguag     1380

aguucaaaga cgcacacgca aaaagacaaa caguaguagu ucuuggaagu caagaaggag     1440

cuguacacac agccuuagcu ggugcacuag aagcagaaau ggauggagca aaaggaagau     1500

uaaguagugg acacuuaaaa uguagauuaa aaauggauaa auuacgauua aaaggaguuu     1560

cauauucauu augcacagca gcuuuuacgu ucacgaagau accagcagaa acacugcacg     1620

gaacaguaac aguagaagua caauaugccg guacagaugg accuuguaag guaccagcac     1680

aaauggcugu ugacaugcaa acauuaacuc ccguugguag acuaauaaca gcaaauccag     1740

uaauaacuga aagcacagaa aauucuaaaa ugaugcuaga auuagaucca ccuuuuggag     1800

auagcuacau cguaauagga guuggcgaaa agaaaauaac ucaucacugg caccguucag     1860

ggucaaccau ugguaaagca uuugaagcaa caguaagagg agccaaaaga auggcgguac     1920

uaggagauac ggcaugggau uuuggaagug uaggaggugc ccuaaauucc uuggguaaag     1980

gaauacacca aaucuuugga gccgcauuua aaucauuauu cgggggaaug aguugguuca     2040

gccaaauauu aauuggaaca cuauuaguau gguuaggauu gaauacuaaa aauggaucua     2100

uaucacuaau gugccuagca uuaggaggag ugcucauauu ccuaaguacu gcuguuucag     2160

cagauguagg auguagugua gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7035
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 2 opt

<400>  7035
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa aaaaaacgaa gaggaacaga uacaagcgug gguauaguug      120

gcuugcuauu gacuacugca auggcagcug aaguaaccag aagaggaagu gcauacuaca      180

uguaucuaga ucguucugau gccggugaag caaucucauu uccuacaaca uuagguauga      240

acaaauguua uauacaaaua auggaucuag gucauaugug ugaugcaaca augucuuaug      300

aauguccuau gcuggacgag ggaguugaac cagaugacgu cgauugcugg ugcaauacca      360

cguccacaug gguaguuuau ggaacuuguc accacaaaaa aggagaagca agaagaagua      420

gaagagcagu aacgcuacca agucauagua caagaaaacu ccaaacaagg ucacagacuu      480

ggcucgaaag uagggaauau acuaaacauc uaauacgagu agaaaauugg auauuuagaa      540

auccaggguu ugcguuagca gcagcagcaa uagcuugguu auuaggcucc aguacuucac      600

aaaaaguaau cuacuuagua augauauuau uaauagcccc agcauauuca auaagaugua      660

uagguguuag uaauagagau uuuguagaag gaaugucagg agguacuugg guagaugucg      720

uucuagaaca cggaggaugc guaacaguaa uggcacaaga uaaacccacu guagauauag      780

aacuaguuac aacaacugua uccaauaugg cagaaguaag aaguuauugu uacgaagcua      840

gcauaagcga uauggcaagu gacucaagau gucccacaca aggugaagca uaccuugaua      900

aacaaucaga uacucaauau guaugcaaac guacacuggu agaccgugga ugggggaaug      960

gaugcggacu auucgguaaa ggauccuuag uuacaugugc aaaauuugcu uguucaaaaa     1020

aaaugacugg gaaaucaaua caaccagaaa acuuagaaua uagaauuaug uuaucgguac     1080

acggaucaca gcauucugga augauaguaa augauacagg acaugaaaca gaugaaaauc     1140

gagcuaaagu agaaauaacu ccuaauaguc caagagcgga agcaacucua ggagguuuug     1200

gaagucuagg auuagauugc gaaccgagaa cuggguuaga cuuuucagau uuauauuauc     1260

uaacuaugaa caacaagcac ugguuaguac auaaagagug guuucaugau auaccgcuac     1320

cauggcaugc gggagcugau acaggaacuc cacauuggaa uaauaaagaa gcacuagugg     1380

aauuuaaaga ugcacaugca aaacgacaaa ccgugguagu acuaggauca caagaaggag     1440

caguacauac agccuuagcu ggugcauuag aagcagaaau ggauggagca aaaggaagau     1500

uaaguagugg ucaucuaaaa uguagacuaa agauggacaa auuaagauua aagggagugu     1560

cuuacagucu auguacagca gccuuuacau uuacaaaaau accugcugaa acauuacacg     1620

gaacaguaac uguggaagua caauaugcag guaccgaugg accauguaaa guuccugcac     1680

aaauggcugu agauaugcaa acucucacac cgguaggacg acuaauaaca gcaaauccag     1740

uaaucacaga aaguacugaa aauaguaaaa ugaugcucga guuagaucca cccuuuggcg     1800

acagcuauau aguaaucgga guaggugaaa aaaaaauuac acaccacugg cacaggagug     1860

gaagcacaau aggaaaagcu uucgaagcaa caguuagagg ugcaaaaaga auggcuguac     1920

ugggagauac agcaugggac uuuggaagug uaggaggagc acuaaauagc uuaggaaaag     1980

gaauacacca aauauuugga gcagcauuua aaucacuauu uggagguaug aguugguuuu     2040

cgcaaauuuu aauaggaaca uuauuaaugu gguuagggcu aaauacaaaa aauggaagua     2100

uaucauuagu uugucuagcg cucggaggag uacuuauauu uuuaagcaca gcaguaucag     2160

cagacguugg auguagugug gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7036
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 2 opt

<400>  7036
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa aaaaaaagaa gaggaacaga cacaaguguu ggaauuguug      120

guuuacuacu uacgacagcu auggcaguag agguuacaag gagagguagu gcauauuaua      180

uguaccuaga uagaucagau gcaggagaag ccauaucauu uccgaccacu uuaggaauga      240

auaaauguua uauacaaaua auggaucuag gacauaugug ugaugcaaca augaguuacg      300

aaugcccaau guuagaugaa ggaguagaac cugaugaugu agauugcugg uguaacacua      360

ccucaacaug gguuguauac ggaacauguc aucauaaaaa aggugaagcu agacgaagca      420

gacgagcugu aacguuacca agucauucaa cacgaaaacu acaaacuaga ucacagacau      480

ggcuugaauc aagagaauau acaaaacacu uaauuagagu ugaaaauugg auauuuagaa      540

auccaggauu ugcacucgca gcagcagcaa uugcauggcu ccuuggcagu aguacaucac      600

aaaaaguaau auauuuaguu augauauuau uaauagcucc agcauacagu auucgaugca      660

uaggaguuuc aaacagagac uuuguagaag gaaugucagg aggcacaugg guagacguag      720

uacuagaaca cggcggaugu guuacaguua uggcccaaga uaaaccagcc guagauauug      780

aauuaguuac uacgacagua aguaauaugg cagaagugag aucauauugu uaugaagcaa      840

guauaucaga uauggcguca gauagccguu gccccaccca aggcgaagcu uaucuagaca      900

aacaaaguga cacacaguac guauguaaaa gaacauuagu agacagggga uggggaaaug      960

gaugcggacu auuuggcaaa ggaagcuuag uaacaugcgc aaaauucgcu uguagcaaaa     1020

aaaugacagg aaaaucaaua caaccagaaa acuuagaaua uagaauuaug uugucaguac     1080

acgggaguca acauuccgga augauaguaa augacacugg ucacgaaaca gacgaaaaua     1140

gagcaaaagu agaaauaacg ccaaauucuc cuagagcaga agcaacacua ggaggauuug     1200

ggucuuuagg guuagacugu gagccaagaa caggauuaga uuuuucugau uuauauuauu     1260

uaacaaugaa uaacaaacau ugguuaguac auaaagagug guuccaugac auaccucuac     1320

cauggcaugc aggagcagau acuggaacuc cacacuggaa uaacaaagaa gcacuuguug     1380

aauuuaaaga cgcucaugca aaaagacaaa ccguaguugu auuaggauca caagaaggug     1440

cggugcacac agcuuuagca ggggcccuag aagccgagau ggauggugca aaagguagac     1500

uuagcucugg ucaccuuaaa ugccgacuaa aaauggauaa auuaagacua aagggaguaa     1560

guuauagccu auguaccgca gcguuuacau uuacaaaaau accagcagaa acacuacaug     1620

gaaccgugac aguagaaguu caauacgcag gaacagaugg uccauguaaa guaccagcac     1680

aaauggcagu agauaugcaa acauuaacac cagugggaag auuaauaaca gcaaacccag     1740

uaauaacaga aaguacagaa aauucaaaaa ugauguuaga auuagacccc ccuuuuggag     1800

auagcuauau agucauaggu guuggcgaaa aaaaaauaac acaucauugg cauagaagcg     1860

guucaacaau aggaaaggca uuugaagcaa cgguacgagg ugcgaaaagg auggcuguau     1920

uaggagauac ggcaugggau uuuggaucag uaggaggagc guuaaauuca uuaggcaaag     1980

gaauacauca aauauucgga gcugcguuua aaucauuauu uggugguaug ucaugguuca     2040

gucaaauauu aauaggaaca cuuuuagucu ggcuagguuu aaacaccaaa aaugguagca     2100

uaucauuaac augcuuagca uuaggaggag uauuaauauu ccucagcaca gcgguuucag     2160

cagacguagg auguagugua gauuuuagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7037
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 2 opt

<400>  7037
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc cagaaaagaa aaaaaaagaa gaggagcgga cacuucagua gguauaguag      120

gacuauuauu aacaacagcg auggccguag aaguaacaag aagaggaagu gcauauuaua      180

uguacuuaga uagaagcgac gcaggagaag cgaucaguuu cccaacaaca uuagguauga      240

auaaauguua uauacaaaua auggaucuug gacauaugug ugacgcaacu augucauacg      300

aauguccaau gcuagacgaa ggaguagaac cugaugacgu cgauuguugg uguaauacaa      360

cuagcacaug gguaguauau ggaacauguc aucauaaaaa gggugaagcu agaagaagua      420

gaagagcagu aacgcuucca ucacacagua caagaaaauu acaaacuaga agucaaacau      480

ggcuagaauc uagagaauau acuaaacauc ucauacgagu agaaaacugg auauuucgua      540

auccgggauu ugcauuggcu gcggcuguaa uagccugguu auuaggaagu aguacaaguc      600

aaaaagugau auauuuagua augauacuac uuauugcucc agcuuacagu auaagaugca      660

ucggaguauc aaauagagau uucgucgaag gaaugagugg agggacaugg guagacguag      720

uacuagaaca uggaggcugc guaaccguaa uggcacaaga uaaaccaaca guagauauag      780

aauuaguaac aacgacaguu uccaauaugg cugaaguacg aucauacugu uaugaagcca      840

gcauuaguga cauggcaucu gauucuaggu gucccacuca aggugaggca uaucuagaca      900

aacaaucaga uacacaauau guauguaaaa gaacauuagu agauagagga ugggggaaug      960

gaugugggcu auuuggaaaa ggaucguuag ucacaugcgc gaaauuugcu uguucaaaaa     1020

aaaugacagg caaaucaauu caaccugaga auuuagaaua uagaaucaug uuaucaguuc     1080

auggaucaca acacagcgga augauaguaa augauacggg acaugaaacu gacgaaaaua     1140

gagcaaaagu ugaaauaacu ccaaauuccc cuagagcaga agcaacacua gguggauuug     1200

gaucauuagg acuagauugc gaaccaagaa caggauuaga uuuuagcgau cuauacuauu     1260

uaacaaugaa uaacaaacau ugguuaguac auaaagaaug guuucaugau auaccauuac     1320

cauggcaugc aggggcagac acugguacac cgcauuggaa uaauaaagaa gccuuaguag     1380

aauuuaaaga cgcacaugca aaaagacaaa caguugucgu auuagguucu caagaaggag     1440

caguacauac ggcuuuagca ggagcucucg aagcugagau ggacggagca aaaggcagac     1500

uuucaagugg acaucucaaa uguagguuaa agauggauaa acuucgauua aaaggaguaa     1560

guuacucccu auguacugca gcauuuacuu uuacaaaaau accagcugaa acuuugcaug     1620

gaacaguaac aguagaagua caauacgcag gaacggacgg accaugcaaa guuccagcac     1680

aaauggcagu agauaugcaa acacugacgc caguaggaag guuaauuaca gcgaacccag     1740

uaauuacaga aucuaccgaa aauucaaaaa ugauguuaga auuagacccc ccuuuuggag     1800

acucuuacau aguuaucgga guaggugaaa aaaaaauaac acaccauugg caucguagug     1860

guuccacaau aggaaaagcu uucgaagcaa cuguaagggg agcaaaaaga auggcaguuu     1920

uaggagacac ggcaugggac uucggaagug uaggaggggc acugaauucu cuaggaaaag     1980

gcauacauca aauauuuggu gccgcauuua aaucauuauu uggaggcaug aguugguuuu     2040

cucaaauauu aauugguaca uuauuaguau ggcuaggauu gaauaccaaa aacggaucca     2100

uaucacuaac augucuagca cuuggaggag uucuaaucuu ucuaucaaca gcuguaucag     2160

cagauguagg cuguucugua gauuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7038
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 2 opt

<400>  7038
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauuaaugc aagaaaagag aaaaaaagaa gaggagcaga uacaaguguu ggaauaguag      120

gauuacuacu aacaacagca auggcagugg aaguaacacg cagaggaucu gcauauuaua      180

uguaucuaga uagaagugau gcuggagaag caauaaguuu uccgacaacu cuaggaguaa      240

acaaauguua uauucaaaua auggaucuag gacacaugug cgaugcaacu augucauacg      300

aguguccuau guuagaugaa ggugucgaac cugaugaugu agacuguugg uguaauacaa      360

cauccacgug gguagucuau ggcacauguc aucauaaaaa aggagaagcu agacgaucaa      420

gaagagcagu uacucuacca agccacucga caagaaaacu acaaacaaga ucacaaacau      480

ggcuugaauc aagagaauau acaaaacauc uaauaagagu cgaaaauugg auauucagaa      540

auccaggauu ugcauuagca gccgcagcaa uagcaugguu auuaggcuca aguacaagcc      600

aaaaaguaau auaucuagua augauacucu uaauagcacc agcauacucu auaagaugca      660

uugguguuag uaacagagac uuugucgaag gaaugucggg aggaacuugg guagauguag      720

uacuagaaca ugguggaugu guaacaguaa uggcacaaga uaaaccaaca guagauauag      780

aauuaguuac aacaacggua ucaaauaugg cagaaguuag aagcuacugu uaugaagcaa      840

guauuucaga cauggcauca gacucaagau guccuacaca aggagaagca uaucuugaua      900

aacaaagcga uacacaguac guauguaaaa gaacacuagu ugauagagga ugggguaaug      960

gauguggucu auuuggaaaa ggaaguuuag uaacuugugc aaaauuugca uguucuaaaa     1020

aaaugacagg aaaaucaauu caaccugaaa acuuagaaua uagaauaaug uuaucuguac     1080

auggauccca acauucagga augauuguaa augauacagg ucaugaaaca gaugaaaaua     1140

gagcuaaagu agaaaucacu ccgaacucac cacgagcaga agcuacuuua ggaggauucg     1200

ggucauuagg ccuugauugc gaaccaagga ccggacuaga uuuuucagau uuauacuacu     1260

uaacuaugaa uaauaaacac ugguuaguac acaaggaaug guuucaugac auacccuuac     1320

cauggcaugc aggagccgac acaggaacuc cacauuggaa uaauaaagaa gcacuaguag     1380

aauucaaaga ugcucaugcc aaaagacaaa caguaguagu uuuaggaagc caagaaggcg     1440

cuguccauac agccuuagca ggagcacucg aagcagaaau ggauggagcc aaaggaagac     1500

uaucaucagg ucauuuaaaa ugucgucuua aaauggauaa auuaagacuu aaagguguua     1560

guuauagcuu auguacagcg gcauuuaccu uuacaaaaau accugccgaa acacuucacg     1620

gaacaguuac aguugaagua caauaugcag ggacagacgg accaugcaaa guuccugcac     1680

aaauggcagu ugauaugcaa acauuaacac caguaggcag auuaauaaca gcaaacccag     1740

uuauaacuga aucaaccgaa aacaguaaaa ugauguuaga auuagaucca ccuuuuggcg     1800

acaguuauau aguaauagga guaggugaca aaaaaauaac ucaucacugg uacagaagug     1860

gaucaacuau agggaaagca uuugaagcaa caguaagagg ggcaaaaaga auggccguau     1920

ugggagauac ggcaugggac uuuggcucag uaggcggugc uuugaauagu uuaggaaaag     1980

guauacauca aauauuugga gcagcauuua aaucauuguu uggaggaaug aguugguuuu     2040

cucaaauuuu gauugguaca uuauuagugu gguuaggacu aaauaccaaa aacggaagua     2100

ucagccuaac uugcuuagcc cuaggggggg uacuaauauu uuuaaguaca gcagugagug     2160

cggacguagg augcaguguc gacuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7039
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 2 opt

<400>  7039
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uaaucaaugc aagaaaagaa aaaaaaagaa gaggugcuga cacauccguc ggaauaguag      120

gacuguuacu aaccaccgcg auggcuguag aaguaacacg acgcggcagc gcauauuaua      180

uguacuuaga uagaagugau gcagguaaag caaucuccuu uccaacaaca uuaggaguaa      240

auaaauguua cauacaaaua auggauuuag gacauaugug ugaugcaaca augucauaug      300

aauguccuau gcuggaugaa ggaguagaac cugaugacgu ugauuguugg ugcaauacca      360

cuucaacaug ggucguauau ggaacauguc aucauaaaaa aggagaagcu agaagaagca      420

gaagagcggu uacucuucca agucauagua cuagaaaacu acaaacaaga ucucaaacuu      480

ggcuugaauc aagggaauac accaaacauu uaauaagagu agaaaauugg aucuucagaa      540

auccaggauu cgcacuagca gcagcggcaa uagcauggcu acuugguuca ucuacaagcc      600

aaaaaguaau auauuuaguc augauacuau uaauagcacc cgcauauucu aucagaugca      660

uaggaguaag uaauagagau uuuguagagg gaauguccgg aggaacaugg guagauguag      720

uccucgaaca ugguggaugu guuacaguga uggcacaaga caagccaaca guagauauag      780

aauuaguuac gacaacuguc ucaaauaugg cagaaguaag aagcuauugu uaugaagcaa      840

gcauaucuga uauggcaagc gauucaagau guccuacuca aggagaagcg uauuuagaua      900

aacagucaga cacacaguac guauguaaac gaacacuagu agacagagga uggggaaacg      960

gauguggauu auucggaaaa gguaguuuag uaaccugugc aaaauuugcg uguucaaaaa     1020

aaaugacugg aaaaaguaua caaccagaaa auuuagaaua uaggauaaug cucagcgugc     1080

auggaucaca acauucagga augauaguaa acgauauagg acaugaaaca gacgaaaauc     1140

gagcaaaagu cgagauaaca cccaauucac cacgagcaga ggcuacccuu ggagguuucg     1200

gaucauuagg auuggauugc gaaccacgaa caggacucga uuuuagugau uuauauuauc     1260

uuacaaugaa caacaaacau ugguuaguac auaaagaaug guuucaugac auuccacuac     1320

cauggcaugc aggagcagau acugguacac cacacuggaa caauaaagaa gcacuaguag     1380

aauuuaaaga ugcucacgcu aaaagacaaa caguaguggu auuaggaagu caagaaggag     1440

caguacauac agcacuagca ggagcccugg aagccgaaau ggauggagca aaaggaaggc     1500

uaucaucagg ccaucuaaaa ugcagacuaa aaauggauaa auugagauua aaaggaguau     1560

cguauagcuu augcacagca gcguuuacuu uuacaaaaau accagcugaa acacuacacg     1620

gaaccguaac uguagaggua caauacgcag gaacagaugg uccauguaaa gucccagcac     1680

aaauggcagu agauaugcaa acauuaacac ccguaggaag gcuuauaacu gcaaauccag     1740

uuauaacaga aaguacagaa aauucuaaaa ugaugcugga auuagaucca cccuucggag     1800

auucauacau aguaauagga guaggagaua aaaaaauuac acaucauugg cauagaucug     1860

ggaguacaau ugguaaagcc uuugaagcaa cagugcgagg cgcaaaaaga auggcaguau     1920

uaggagauac agcuugggau uuuggaucag ugggaggagc auuaaauagu uuaggaaaag     1980

gaauacauca aauauucgga gcagcauuua aaagucuauu uggaggcaug ucaugguuuu     2040

cacaaauucu uauaggaacg cuuuuaguau gguuaggauu aaauacaaaa aauggaucaa     2100

uaucacugac auguuuagcg cuagguggcg uuuuaauauu uuuaagcacg gcaguaucag     2160

cagacguagg augcucagua gauuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7040
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 2 opt

<400>  7040
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa agaaaaagaa gaggagcaga uacaagcaua gguauaguag      120

ggcuauuauu aacaacugcu auggcagcag aaaucacgag aagaggaagu gcauauuaca      180

uguauuugga cagaucugau gcaggaaaag caauaucuuu cgcaacaacu uuaggcguaa      240

acaaauguca uguucaaaua auggacuuag gccacaugug ugaugcgaca augaguuaug      300

aauguccaau guuagaugaa ggaguagagc cagaugaugu ugauuguugg ugcaauacaa      360

caagcacaug ggucguuuac gggacuuguc aucauaagaa aggagaagca agaagaagua      420

gacgagcugu uacuuuacca agccauagua caagaaaauu gcagacaaga agccaaacau      480

ggcucgaaag cagagaauau acaaaacauu uaauaaaagu agaaaauugg auauuccgaa      540

acccaggauu uacgcuggua gcaguagcca uagcuugguu guuaggaucc uccacgucac      600

aaaaagugau auacuuagug augauauuac uaauugcacc agcuuauuca auaaggugua      660

uaggaguauc aaauagagau uuuguagaag gaaugagugg aggaacuugg guagacguag      720

uacuagaaca uggagguugu gugacuguaa uggcacaaga uaaaccgacu guugauauag      780

aacuuguaac aacaacugua ucaaauaugg cugaaguaag aucauacugc uaugaagcau      840

cuauaaguga uauggccucc gauucaaggu gcccaacaca aggggaggcu uacuuagaua      900

aacaaaguga uacacaauau guauguaaac gaacauuagu agauagagga ugggguaaug      960

guugcggauu auuuggaaaa ggaaguuugg uaacaugugc uaaauuuacu ugcaguaaga     1020

aaaugacagg caaaagcaua caaccggaaa aucuggaaua uagaauaaug uuaucaguac     1080

auggaucaca acauagugga augauaguaa augacacugg gcaugaaaca gaugaaaaua     1140

gagcaaaagu agaaguuaca ccgaacagcc caagagcgga agcaacuuua gguggguucg     1200

gaucccuagg acuagacugu gaaccaagaa caggcuugga uuuuagugac uuauauuacc     1260

uaacaaugaa caauaaacau uggcuaguuc auaaagaaug guuucacgau auaccauuac     1320

cauggcacgc aggagcagau acaggaacac cacauuggaa uaauaaagaa gcgcuagucg     1380

aguucaaaga ugcacacgca aaacgacaaa caguagucgu acuugguuca caagaaggag     1440

caguucacac ugcuuuagcg ggugcauuag aagcagaaau ggauggagca aaaggaagac     1500

uauuuucagg acaucuuaaa ugccgucuua aaauggauaa auuaagacuu aaaggaguaa     1560

guuauucacu augcacagca gcauucacau uuacuaaagu uccugcagaa acauuacacg     1620

gaacagugac aguugaaguc caauacgcug gaacagaugg uccauguaag gucccagcac     1680

aaauggcggu agauaugcaa acauugacac caguuggaag acuaauuaca gcaaauccag     1740

uaauaacaga aaguacagaa aauaguaaaa ugauguuaga auuggaccca cccuuuggag     1800

auaguuauau aguuauagga guaggagaua agaaaauaac acaucacugg cauagaucug     1860

gaucaacgau aggaaaagca uuugaagcaa cuguaagagg agcuaaaaga auggcaguac     1920

uuggagauac agcaugggac uucggcucag uaggcggugu cuucaauuca uuaggaaaag     1980

gaauacauca aauauuuggc gcagcauuua aaucacuauu cggagguaug aguugguuuu     2040

cacaaauacu uauaggaacc cuuuuaguuu ggcuaggacu aaauacuaaa aaugguucaa     2100

ucaguuuaac auguuuggca uuaggaggag uuaugaucuu ucuaagcaca gcuguuagug     2160

cugauguagg guguagcgua gauuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7041
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 2 opt

<400>  7041
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc aagaaaagag agaaagagac gaggagccga cacaucuaua ggaauaauag      120

gacuacuauu aacaaccgca auggcagcag aaauaacucg aagagggagu gcauacuaca      180

uguauuuaga ucgaucagac gcugguaaag ccauaaguuu ugcgacuaau uuaggaguaa      240

acaaaugcca uguucaaauu auggaccuag gacauaugug cgaugcaaca augaguuacg      300

aauguccaau gcuagaugaa ggaguagaac cagacgaugu agauugcugg uguaauacaa      360

caucaacaug gguuguauac ggaacauguc aucauaaaaa aggagaagca agacgaucaa      420

gaagagcagu aacuuugccc ucucacagca caagaaaauu acagacccga agccaaaccu      480

ggcuagaauc aagagaauau acaaaacauc uuauaaaagu ugaaaauugg auauuuagaa      540

aucccgguuu ugcguuagca gcaguagcua uagccugguu acuuggaagc aguacaucac      600

aaaaaguuau auauuuaguu augauauuau uaauugcgcc agcuuauuca auuagaugua      660

ucggaguaag caaccgggau uucguugagg gaaugucagg ggguacaugg guagauguag      720

uauuagaaca ugggggaugc guaacaguaa uggcgcaaga uaaaccaaca guagacauag      780

aauuaguaac aacgaccgug ucaaacaugg cagaaguucg cucauauugu uaugaagcaa      840

guauaucuga uauggcauca gacucaagau guccuacaca aggugaagca uacuuagaca      900

aacaaucuga uacucaguac gucuguaaac gaacucuagu agacagagga uggggaaaug      960

gaugcggucu auuugguaaa ggaucacuag ugacuugugc aaaauuuaca uguagcaaaa     1020

aaaugacagg uaaaaguauu cagcccgaaa accuugagua uagaauaaug uuaucagucc     1080

auggaucuca acauaguggu augauaguca augauauagg ccaugaaaca gaugaaaaua     1140

gagcaaaagu ugaaguaaca ccaaauucac caagagcaga agcaacccua gggggauuug     1200

gaucacuugg auuagauugu gaaccuagaa cuggacuaga cuuuucagac uuauacuauu     1260

uaacaaugaa caauaaacau ugguugguac auaaagaaug guuucacgau aucccuuuac     1320

ccuggcaugc cggagccgau acaggaaccc cacauuggaa uaauaaagag gcacuaguug     1380

aauuuaagga ugcacaugcg aaaagacaaa caguaguagu acuaggauca caagagggag     1440

ccguacauac ggccuuagcu ggagcacuag aagcagaaau ggacggagcu aaaggaagac     1500

uauuuagcgg gcacuugaaa ugccgauuaa aaauggauaa auuacgacuu aaaggaguuu     1560

ccuacucauu augcacagcu gcauuuacgu uuacuaaagu accugcagaa acauugcacg     1620

gaacaguaac aguagaagua caauaugcug guacagaugg acccuguaaa gugccagcgc     1680

aaauggcugu ggauaugcaa acauuaacuc cuguuggaag acuaauaaca gcaaauccag     1740

uaauaacaga aucaacugaa aacaguaaaa ugauguuaga auuagacccu ccauuugggg     1800

auucguauau aguaauaggu guuggagaua aaaaaauaac acaucauugg cauagaagug     1860

ggucgacaau aggaaaagcu uucgaagcaa caguaagagg agcaaaaaga auggcagucu     1920

uaggagacac agcaugggau uuuggaucag uuggaggugu cuucaauagu uuagguaaag     1980

gaguacacca aaucuucgga gcagcuuuca aaagucuauu ugggggaaug ucaugguuuu     2040

cacaaauacu uauuggaaca uuacuagucu gguuaggacu caauacaaaa aauggcagua     2100

uaagcuuaac auguuuggca uuaggaggcg ugaugauuuu ccuaagcaca gcaguaucag     2160

cagacguagg uuguaguguu gauuucagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7042
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 2 opt

<400>  7042
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaacgc aagaaaagaa cgaaaaagaa gaggagcaga cacaucaauu ggaauaauag      120

gucuguuauu aacaacagcc auggccgcag aaauuacaag aagaggcagu gcauauuaua      180

uguauuuaga ccgaagugau gcuggaaaag caauaucauu cgcuacuaca uugggaguca      240

auaaauguca uguccaaauu auggaccuug gucauaugug cgacgcaacu augucauaug      300

aauguccgau guuagaugag gguguugaac cagacgacgu agauuguugg ugcaacacaa      360

caaguacuug gguaguauau ggaacaugcc aucauaaaaa aggagaggca cgccgaagua      420

gaagagcagu aacacuacca ucucacucaa caagaaaauu acaaacuagg ucacaaacau      480

gguuagaguc acgcgaauac acaaaacauu uaauaaaagu agaaaauugg auauuuagaa      540

auccaggauu ugcacuugua gccguagcca uagcuuggcu acuugguagc ucaacaaguc      600

aaaaaguaau auauuuagua augauauuac uaauagcccc agcauacagu auaaggugca      660

uagguguauc aaauagagau uuuguagaag guaugucagg cggaacaugg guagauguag      720

uacuugaaca cggaggaugc guaacaguaa uggcgcaaga caaacccaca guagacauug      780

aacuaguaac aaccacagua agcaauaugg cugaaguaag aucuuauugu uacgaagcaa      840

guauaagcga uauggcaagu gauucaagau guccuacaca aggcgaagca uauuuggaua      900

agcaaaguga cacucaauau guuuguaaaa gaacuuuagu cgacagagga uggggaaaug      960

gaugcggacu uuuuggaaaa ggaagccuag uaacaugcgc caaauucaca uguaguaaaa     1020

aaaugacagg aaaaaguaua caaccugaaa accuagagua cagaauaaug cuuaguguac     1080

acggaagcca acacuccgga augauuguaa augauauugg ucaugaaaca gacgaaaaua     1140

gagcaaaagu agaaguuacc ccaaacucac cuagagcaga ggcaacacua gguggauuug     1200

gcucauuagg auuagauugu gaacccagaa cagggcuaga cuuuagugac uuauauuauc     1260

uaacgaugaa caauaaacau ugguuagucc auaaagagug guuccaugau auaccccuac     1320

cauggcaugc gggagcagau acugguacac cacauuggaa caacaaagaa gcauuaguag     1380

aauuuaaaga cgcucaugcu aaaagacaaa cuguaguagu auuaggauca caagaaggug     1440

cagugcacac agcauuagca ggugcucuag aagcagaaau ggauggagca aaaggaagau     1500

uauucucagg gcaucuaaaa ugccgauuaa aaauggauaa auuaagauua aaagggguaa     1560

guuauucauu auguacagca gcauucacuu uuacuaaagu accagcagaa acauuacacg     1620

guacagucac aguagaagua caauaugcag gaacagaugg accaugcaaa guaccagcac     1680

agauggcagu agauaugcaa acauuaacac caguuggacg uuuaauaaca gcgaauccag     1740

uaauaacaga aucaacagaa aauaguaaaa ugaugcuaga guuggauccg ccauuugggg     1800

auucuuacau aguuauagga gugggugaca aaaaaauaac ucaccauugg cacagaagcg     1860

guucaacaau aggaaaagca uuugaagcaa caguaagagg agccaaacga auggcaguac     1920

ucggagauac agcaugggau uuuggaucag ucggaggugu auuuaacuca uuaggaaaag     1980

guauacauca gauauucggg gccgcuuuua aaucauuauu uggcggaaug ucaugguuca     2040

gucaaauauu aauuggcacu uuauugguuu ggcuaggacu uaauacaaaa aaugggagua     2100

uaagccuuac cuguuuagcg uuaggaggag uuaugauauu uuugucaacc gcaguaagug     2160

cggauguagg augcagugua gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7043
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 2 opt

<400>  7043
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uuauaaacgc aaggaaagag agaaaaagac guggagccga uacaagcauc ggaauaauag      120

gccuauuacu aacaacagcc auggcagcug agauuacaag aagaggaagc gcauauuaca      180

uguauuuaga uagaagugau gcuggaaaag caauaucauu ugcaacaaau uuaggaguaa      240

auaaauguca uguccaaaua auggacuuag ggcacaugug cgaugcaaca augagcuaug      300

aauguccaau guuagaugaa ggagucgaac cagaugacgu agauuguugg uguaauacaa      360

caucaacaug gguaguauac gguacuugcc aucauaagaa aggagaagcg agaagaucaa      420

gacgagccgu gacucucccg agucacagua cacguaaauu acaaacucga ucacaaacau      480

gguuagaauc ucgagaauau acaaaacauu uaaucaaagu agaaaacugg auauucagaa      540

auccaggauu cgcacuagcc gcaguagcaa uagcaugguu auuagggagu aguacuucuc      600

aaaaaguaau cuaucucgua augauccuau uaauugcacc agcauauagc auacguugca      660

uaggaguaag caacagggau uuuguggaag guaugagugg aggaacaugg guagauguag      720

uauuagaaca cggagguugc guaacuguaa uggcccaaga caaaccaaca guagauauag      780

aacucguaac aacaacagua ucaaauaugg cagaaguaag aaguuauugu uaugaagcca      840

guauuucaga uauggcauca gacuccagau gcccgacaca aggggaagcc uauuuagaca      900

agcaaaguga uacacaauau guauguaaac gaacauuagu ugauagggga ugggguaaug      960

gauguggauu auucggaaaa ggaucauuag uaacaugugc aaaauucacu uguuccaaga     1020

aaaugaccgg aaaaucaaua caacccgaaa acuuagaaua cagaauaaug cuaucuguac     1080

auggaucaca acacagugga augauaguaa augacgaaaa uagggcaaaa guagaaguca     1140

caccaaacag cccucgagcg gaagcaacau uaggaggauu uggaagucua ggauuagacu     1200

gugaaccaag gacuggucua gauuuuucgg accuauauua uuuaaccaug aauaacaaac     1260

auugguuagu acauaaagaa ugguuucaug auauuccauu accguggcau gcuggugcag     1320

auacaggaac accgcauugg aacaauaaag aagcauuagu agaauuuaaa gaugcacaug     1380

caaaaagaca aacaguagua guuuugggca gccaagaagg ugcaguucau acugcacugg     1440

cuggagcucu cgaagcagaa auggauggag ccaaaggaag gcuauuuuca ggacacuuga     1500

aauguaggcu aaaaauggau aaacuaagau uaaaaggcgu aucauauucu cuauguacag     1560

cggccuuuac cuuuacaaaa guuccagcag aaacucuaca uggcacaguu accguagaag     1620

uacaauaugc aggcacagau ggaccaugca aaguaccagc acaaauggca guagacaugc     1680

aaacauuaac acccguaggg agauuaauaa cagcaaaucc aguuauaacu gaaucaacug     1740

aaaacagcaa aaugauguug gaacuagauc caccuuucgg cgauaguuac auaguaauag     1800

guguaggaga uaagaaaaua acacaucacu ggcaucguag uggaagcaca auaggaaaag     1860

cauuugaagc aacaguaaga ggggcuaaaa gaauggcagu acuaggagau acagcuuggg     1920

acuucggauc aguagggggu guauuuaaua gcuuaggaaa aggcauacac caaauauuug     1980

gagcagcauu uaaaaguuua uuugggggaa ugaguugguu cucacaaaua uuaauaggaa     2040

cuuuauuggu uugguuaggu cucaacacua aaaacgguag uauaucauua acaugucuag     2100

cacuaggagg aguaaugauc uuuuuaucaa cagcaguauc agcagacgua gguuguagug     2160

uagauuuuuc aaaaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7044
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 2 opt

<400>  7044
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauuaaugc aagaaaagaa agaaaacgua ggggagcaga cacaucaauu ggcauugucg      120

gauuauuauu aacaacugcu auggcagcag aaaucacaag aagagguagu gcuuauuaua      180

uguauuuaga uaggagugau gcaggaaaag caauaucauu ugcaaccaca cuaggcguaa      240

auaaauguca ugugcagaua auggauuuag gacauaugug cgaugcuacu augaguuaug      300

aauguccaau guuagaugaa ggaguagaac ccgaugaugu agacuguugg uguaacacaa      360

cgaguacaug ggugguauau ggaacauguc aucauaagaa aggagaagca agaaggagua      420

gaagggccgu uacacuaccu agucauagua caagaaaauu acaaacaaga agccaaacau      480

gguuggaaag ccgcgaauac acaaaacauu uaauaaaagu agaaaauugg auuuucagaa      540

auccgggcuu ugcccuagua gcaguagcaa uugcaugguu acuaggaagu uccacaaguc      600

aaaaaguaau uuauuuagua augauauuac uaauagcgcc agcauacucu auaaggugua      660

uuggaguaag uaauagagau uuuguagaag gcaugagugg aggaacuugg guagauguag      720

uacuagaaca uggaggaugu gucacaguga uggcccaaga caaacccaca guagauauag      780

agcuaguaac aacuaccgua aguaacaugg cggaaguaag aucauacugu uaugaagcaa      840

guauaucaga cauggcuucc gauaguagau guccgacaca aggagaagcg uaccuggaua      900

aacaaagcga cacacaauau guauguaaaa gaaccuuagu agauagagga uggggaaaug      960

guugugguuu auuuggaaag gguagcuuag uaacaugugc aaaauuuaca uguucaaaga     1020

aaaugacggg aaaaucaaua caaccugaaa acuuggaaua uagaauaaug cuaaguguac     1080

auggaucaca acauagugga augauaguaa augauacagg acaugaaacg gaugaaaauc     1140

gagcaaaagu ggaaguaacg ccaaacucac caagagcgga agcgacauua ggagguuucg     1200

gaucauuagg guuggacugu gaaccacgaa caggacuaga uuucagugau uuauacuauu     1260

uaacuaugaa uaacaaacau ugguuaguuc auaaagaaug guuucaugau auaccauuac     1320

cauggcacgc aggcgcagau acgggaacac cacauuggaa caauaaagaa gccuuagucg     1380

aauuuaaaga ugcacacgcg aaacgccaaa cuguaguagu uuuaggauca caagaaggag     1440

caguacauac agcauuagcu ggugcauugg aagcggaaau ggauggagca aaaggaagau     1500

uauuuagcgg ucauuuaaaa ugucgacuaa aaauggauaa auuaagauua aagggaguau     1560

cauauucacu auguacagca gcauuuacuu uuaccaaagu uccagcugaa acauuacaug     1620

gaacgguaac uguagaagua caauaugcag gaacugaugg accuuguaaa guaccugcuc     1680

agauggcagu ugauaugcaa acauuaacac cuguaggacg auugauaacc gcaaauccag     1740

ugauaacaga aucuacagaa aauaguaaaa ugauguugga auuagaucca ccuuuuggug     1800

acaguuauau uguaauagga guaggugaua agaaaauuac acaccacugg cacaggagug     1860

guaguacuau aggaaaagcc uuugaagcua caguaagagg agcaaaaaga auggcugugu     1920

uaggagauac agcuugggac uuugguucag uuggcggagu uuuuaauucg uuagggaaag     1980

gcauucauca aauauucggu gcagcauuca aaagccuauu cggaggaaug agcugguuca     2040

gucaaauacu uauaggaaca cuauuagugu ggcuugguuu aaauacgaaa aauggaucaa     2100

uaaguuuaac auguuuagca uuagguggug uaaugauauu cuuaaguaca gcaguaagcg     2160

cagauguagg auguucugua gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7045
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 2 opt

<400>  7045
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagag cgaaaaagaa gaggagcaga cacaucuauu gggauaguag      120

gacuauuauu aacaacagcg auggcggcug aaauuacaag aagaggaagu gcuuacuaua      180

uguacuuaga ccgaagugau gcuggcaaag caauaucguu cgcuacaaca cuugggguaa      240

acaaaugcca cguucaaaua auggacuuag gacauaugug ugaugcuacg augagcuacg      300

aauguccaau guuagaugaa gguguagaac cagaugacgu agacuguugg uguaacacaa      360

caaguacaug gguaguuuau ggaacauguc aacacaagaa aggggaagca agacguagca      420

gacgagcagu gacacuaccu agucauagua cuagaaaacu acaaacaaga ucacagaccu      480

gguuagaaag uagagaauau acaaaacauc uaauaaaagu agaaaauugg auauuuagaa      540

aucccggauu ugcccuaguu gcaguagcaa uagcaugguu auuaggauca agcacaaguc      600

aaaaaguaau auauuuagua augauauuac uuauagcacc agcuuacagc auuagaugua      660

uagguguauc aaauagagac uuuguggagg guaugagcgg aggaacaugg guugauguag      720

uauuagaaca uggaggcugu guuacuguaa uggcucaaga caaacccaca guagauauag      780

aauuaguaac gacaacagua ucgaauaugg cagaagucag aucuuauugu uacgaagcga      840

gcauaucaga cauggcaagu gauaguagau gucccacuca aggugaagca uauuuagaca      900

aacaaucaga uacacaauau guauguaaaa ggacauuagu agaccggggu uggggaaaug      960

gaugcggacu auuugguaaa ggcagcuuag uuacaugugc caaauuuaca uguucuaaga     1020

aaaugacagg aaaaaguaua caaccagaaa auuuagaaua uagaauuaug uuaaguguac     1080

auggaagcca acauucagga augaucguaa augacauugg acaugagacu gaugaaaaua     1140

gagcaaaagu agagguaacc ccaaauucuc cuagggcaga agcaacacuc ggaggauucg     1200

gaaguuuagg acuagauugu gaaccaagaa caggacuaga uuuuucagau uuauacuauc     1260

uaacaaugaa uaauaaacau uggcuagugc auaaagaaug guuucacgac auuccgcuac     1320

cauggcaugc aggagcagau accggaacac cacauuggaa uaacaaagaa gcucugguag     1380

aauuuaaaga ugcacaugca aaaaggcaaa cgguaguagu ucuuggauca caagaaggag     1440

caguacacac ggcauuagca ggagcauuag aggcugaaau ggauggugcu aaaggucggc     1500

uauuuucagg ucauuuaaaa ugcagguuaa aaauggauaa auugagauua aaaggaguaa     1560

gcuauagucu auguacagcu guuugcacag cugcaaaagu accagcagaa acauuacaug     1620

gaacuguaac aguagaagua caauacgcag gaacagaugg accuuguaaa guaccagcac     1680

aaauggcugu agauaugcaa acacuuaccc caguaggacg auuaauuaca gcaaaccccg     1740

uaauaacaga gucaacggaa aauaguaaaa ugauguuaga auuagacccu cccuuuggug     1800

auucauacau aguaauagga guaggagaua agaaaauaac acaccauugg cacagaucag     1860

gcucaacaau cggaaaagcc uucgaagcua caguacgagg agcaaaaaga auggcaguau     1920

uaggagauac ugcaugggau uuuggcagug uaggaggagu auuuaauagu uuagguaaag     1980

guauucauca aauuuuugga gcagcuuuca aaucauuauu cggaggaaug aguugguuuu     2040

cacaaauacu uauaggaaca cuauuaguau gguuaggacu uaauacgaaa aacgggucaa     2100

ucagcuuaac auguuuagca cuagguggcg uaaugauauu ucuuucaaca gccguuagug     2160

cagauguagg cuguuccguu gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7046
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 2 opt

<400>  7046
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc uagaaaagaa cggaagagaa gaggagcuga ugcgucaaua ggaauaguaa      120

guuuauuauu aacaacagua auggcugcag aaauuacaag gagaggauca gcuuauuaua      180

uguauuuaga cagaagugau gcgggaaaag caauaaguuu uguaacaaca uuaggaguaa      240

auaaauguca uguccaaaua auggacuugg ggcauaugug cgaugcaaca augucuuaug      300

agugcccaau guuggaugaa ggaguugaac cagaugaugu agauuguugg uguaauacaa      360

caucaacuug gguaguauac ggaacaugcc accacaagaa aggggaagcu agaagaucaa      420

gaagggcagu aacauugcca agucauagua cgagaaaacu acaaacacgu agccagacau      480

ggcuugaauc aagagaauau acaaaacacu ugauaaaagu agagaauugg auuuuccgaa      540

auccagguuu cgccuuaguu gcaguagcaa uugcaugguu acuaggaagc agcacuucuc      600

aaaaaguaau auauuuagua augauauuac uaauagcacc agcauauuca auaagaugua      660

uaggagugag uaacagagau uucguagaag guaugagugg aggaacaugg guagacguag      720

uacucgaaca cgguggaugc guaacaguaa uggcucaaga caaaccaaca guagauauug      780

aacucguaac aacaacagua ucaaauaugg ccgaaguuag gucuuauugc uaugaggcau      840

caauaucaga cauggcauca gauagcagau guccuacaca aggagaagca uauuuagaua      900

aacaaucaga cacacaauac guaugcaaaa gaacauuagu agauagaggc uggggaaacg      960

gcuguggacu guuugggaaa ggaucucucg uaacaugcgc uaaauucacc uguaguaaga     1020

aaaugacagg aaaaaguaua caaccagaaa accuagaaua uagaauuaug uuaagcgugc     1080

auggaagcca acacagcggg augauaguua augaugaaaa uagggcaaaa guagagguca     1140

cgccaaacag cccaagagca gaagcaacau uaggagguuu cggaagccuc ggauuagauu     1200

gugaaccaag gacuggauua gauuucucag auuuauauua ucuuacaaug aauaacaaac     1260

acuggcuagu acauaaagaa ugguuucacg auauuccauu accauggcau ucaggagcag     1320

acacugaaac accacacugg aauaauaaag aagcguuggu agaauuuaaa gaugcacacg     1380

caaaaagaca aaccguagua gucuuagguu cgcaagaagg agcaguacau acagcacuag     1440

caggcgcgcu agaagcugaa auggauggug caaaaggaag acugucauca ggucaucuaa     1500

aauguagauu aaaaauggau aaauuaagac uaaaaggugu aucauauucc uuauguaccg     1560

cagcguuuac uuucacaaaa guuccagccg aaacauuaca uggaacagua acuguagaag     1620

uacaauaugc agggcgagac ggaccuugua aaguuccagc acaaauggca guugauaugc     1680

aaacauuaac accaguuggg agauuaauaa ccgcaaaccc aguaauaacu gaaucaaccg     1740

aaaauucaaa gaugauguua gaauuagauc ccccauuugg agacuccuau auagugauag     1800

gaguuggaga uaagaagaua acccaucauu ggcauagauc aggaagcauu auaggaaaag     1860

cauuugaagc aacaguaaga ggcgcaaaaa gaauggcagu acuaggcgau acggcauggg     1920

acuuuggauc agugggagga guuuucaaca gucuaggaaa agguauucac caaauauucg     1980

gagccgcauu caaaagccua uucggaggua ugucaugguu uucacagauu cuaauaggaa     2040

cgcuucuagu augguuggga uuaaauacua aaaauggaag uauaucacua acaugucuug     2100

cuuuaggagg aguaaugaua uucuugucaa cagcaguuuc ugcagacguu ggaugcagug     2160

uggauuuuag uaaaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7047
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 2 opt

<400>  7047
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaaugc aagaaaagaa agaaaaagaa gaggagcuga uacaucuauu ggaauaguag      120

gcuuguuauu aacaacagcu auggcagcag aaauaacaag aagaggaagc gcuuacuaua      180

uguauuuaga caggagugau gccggaaaag caauaucauu ugcaacaaca uuagggguca      240

auaaauguca uguccaaaua auggacuuag gacauaugug cgacgcaaca augaguuaug      300

aguguccaau guuggacgaa ggaguagaac cagaugaugu agauuguugg uguaauacga      360

cuuccacaug gguaguuuac ggaacuuguc aucauaagaa aggagaagcu agaagaagca      420

ggagagcagu aacacuacca ucacauagua cuagaaaacu ucaaacaaga agccaaacau      480

ggcuagaguc aagagaauau acaaaacacu ugauaaaagu agaaaauugg auauuuagaa      540

auccaggcuu ugcauuagug gcaguagcua uagccuggcu uuuaggaagc aguacaaguc      600

aaaaaguaau auauuuagua augauauuau uaauagcacc agcauauuca aucagaugca      660

ucggagugag uaauagagac uucguagaag guaugagcgg aggaacaugg guagaugugg      720

uacuagaaca cggaggcugu guaacaguaa uggcacaaga uaagccgacc guagauauug      780

aauuaguaac caccaccgug aguaauaugg cagaaguuag aucauauugc uaugaagcaa      840

guaucaguga uauggcaagc gauaguagau guccgacaca aggagaagca uaccuagaua      900

aacaaagcga cacacaauac guauguaaaa gaacacucgu agauagagga uggggaaacg      960

gauguggauu auuugggaaa ggaucccuag uaacaugugc caaauuuacu uguucgaaga     1020

aaaugacugg aaaaaguaua caaccugaaa acuuagaaua uagaauaaug cuaucaguac     1080

augguagcca acauagcgga augauaguaa augaugaaaa cagggcaaaa guagaaguaa     1140

cgccuaauag cccaagagca gaagcuacac uagguggauu uggaaguuua ggacuagauu     1200

gcgaaccacg aacgggguua gauuuuagug acuuauauua uuuaacuaug aacaauaaac     1260

auugguuagu acacaaagaa ugguuucacg acauaccauu acccuggcau gcaggagcgg     1320

auacaggaac accacauugg aauaacaaag aagcucuagu ggaauuuaaa gaugcacaug     1380

caaaaaggca aacaguugua guacugggau cacaagaagg agcgguucau acagcccuag     1440

caggagcacu agaagccgaa auggauggag cgaaaggaag auuauucuca ggacauuuaa     1500

aaugucgauu aaaaauggac aaacuacgau uaaaaggagu uaguuacuca uuauguacag     1560

cagccuucac guuuacaaaa guaccagcag aaacacuaca cggaacagua acuguagaag     1620

uacaauacgc uggaacagau ggaccuugua aaguaccugc ccaaauggcg guagauaugc     1680

aaacauuaac accugugggu agguuaauaa cagcaaaucc aguaauaacu gaaucaacag     1740

aaaacaguaa aaugauguua gaauuagauc cacccuuugg ugauagcuau auagucauag     1800

gaguagguga caagaaaauc acacaucauu ggcaccgaag uggcucaacg auagguaaag     1860

cauucgaagc aacaguucga ggagcuaaaa gaauggcagu acuaggagau acagccuggg     1920

auuuugggag uguaggugga guauucaaua gccuagggaa aggaauacac caaauauucg     1980

gggcagcauu caaaagucua uuugguggua ugaguugguu uucacaaaua uuaauaggca     2040

cuuuauuagu auggcuaggg uuaaauacua aaaacggaag cauuagcuua acaugccuag     2100

cgcugggagg uguaaugaua uuucuaagua cagcaguaag ugcagaugua ggauguagcg     2160

uugauuuuuc aaagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7048
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 2 opt

<400>  7048
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauaaaugc aagaaaagaa agaaaaagaa ggggagcuga cacuucaaua ggaauaauag      120

gauuacuacu uaccacagca auggcagcag aaauaacacg aagaggaucu gcauauuaca      180

uguaucucga uagaagcgac gcagguaaag ccauaaguuu ugcaaccaca uuagguguaa      240

auaaguguca uguacaaauc auggacuuag gacauaugug ugacgcaacc augagcuaug      300

aaugcccuau gcuagacgaa ggaguagaac ccgaugacgu cgacuguugg ugcaacacua      360

caagcacaug ggucguuuau gguaccuguc aucauaagaa aggagaaacu cgaagaucaa      420

gaagaagugu aucucuuaga uaucauuaca caagaaaacu acagacaaga ucucaaacau      480

ggcuagaauc aagagaauau aagaaacauc uaauaauggu ugaaaauugg auauucagga      540

acccaggauu ugcaauaguu aguguugcaa uaacauggcu uaugggaagu uuaacaagcc      600

aaaaaguuau auacuuagua augauaguuc uaauuguacc agcuuauuca auaagcugca      660

uaggagugag uaacagagau uuagucgaag gaaugucagg uggaacaugg guagauguag      720

uauuagaaca ugggggaugc guuacagaaa uggcccaaga uaaaccgaca guagauaucg      780

aauuaguaac aaugaccgua ucaaacaugg ccgaaguaag aucauauugu uaugaagcau      840

cacuaucaga uauggcaagu gcaagcagau gcccaacuca aggggaacca ucucuagaua      900

aacaaucaga cacacaauca guaugcaaaa gaacauuggg ugauagagga uggggcaacg      960

gcuguggcau uuuuggaaaa ggaagucuag uuaccuguag uaaauucacg ugcuguaaga     1020

aaaugccagg aaaaaguauu caaccagaaa accuugaaua cagaauuaug uuaccaguac     1080

auggaucgca acauaguggc augauaguga augauauagg ucacgaaaca gacgaaaaua     1140

gagcaaaagu ugaagugaca ccaaacaguc caagagcaga agcaacauua ggaggauuug     1200

gaucauuagg auuagauugu gaaccacgaa ccgggcuaga uuucagcgau cuuuauuacu     1260

uaacgaugaa uaauaaacac uggcuaguac auaaagaaug guuccaugac auaccauuac     1320

cauggcacgc aggagcagau acuggaacuc cacauuggaa uaauaaagaa gcacuuguug     1380

aguucaaaga ugcacaugca aaaaggcaaa caguaguagu acuaggaagc caagaaggag     1440

cuguucacac agcguuagca ggcgcacuug aagcugaaau ggauggagca aaaggaagau     1500

uguuuucagg acauuuaaag ugcagacuua aaauggacaa acuaagacua aaaggcgucu     1560

cuuacucauu auguacagca gcauuuacau ucacuaaagu gccagcagaa acacuacaug     1620

gaacugucac aguagaaguc caaucugcag guacagaugg cccauguaaa guacccgccc     1680

aaauggcagu ugacaugcaa acgcugacac caguagguag acuaauaaca gcaaacccug     1740

uaaucacaga aucaacagaa aacuccaaaa ugauguuaga acuagaucca ccuuucggag     1800

auaguuauau uguaauugga guaggagaua agaaaauaac acaucauugg cauagaagcg     1860

gaaguacuau aggaaaagcc uucgaagcua caguaagagg cgcuaaaagg auggcugucu     1920

uaggugauac agcuugggau uuuggaucag uagggggugu auucaauagu cuaggaaaag     1980

gaauacauca aauuuuugga gccgcguuca aaaguuuauu cggagguaug ucgugguuuu     2040

cacaaaucuu aauaggaaca uuacugguau ggcuaggacu aaauacuaaa aauggcagua     2100

uuagcuuaac auguuuagcu uuaggaggag uaaugauauu ucuuagcaca gcaguuagug     2160

cggaugucgg auguucugua gacuuuucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7049
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 2 opt

<400>  7049
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuauuaacgc ucguaaagaa agaaaaagac guggugcaga cacaagcaua gggauaauag      120

guuugcuauu aacaacugca auggcugcug aaauaacaag acgaggauca gcauauuaua      180

uguaccugga uaggucugau gcaggaaaag caauaucauu cgcaacuaca augggaguaa      240

auaaauguca uguacaaaua auggauuuag gacacaugug ugaugcuaca augaguuaug      300

aauguccuau guuagacgaa ggaguagaac cugaugaugu ugacuguugg uguaauacua      360

cuagcacaug gguuguauau gggaccugcc aucauaagaa aggagaagca aggccaucaa      420

gacgagcagu cacauuaccg agucauagua cucgaaaauu acaaacucga agucaaacgu      480

gguuagaauc acgugaauac acaaaacacu uaauaaaagu agaaaauugg auacuucgaa      540

acccuggguu uacgguagca gccuuagcaa uggcauggcu ucuaggaaau aguacaaguc      600

aaaggguaau auaccuagua augauuuuau uaauagcacc cgcauauuca auaagaugca      660

uaggaguaag caauagagau uuuguagaag guaugucagg aggaacaugg guagauguug      720

uacuagaaca cggcggaugc guaacaguua uggcucaaga caaaccaaca guagauauag      780

aauuaguaac uacaacagua ucaaauaugg cugaaguaag gaguuauugu uaugaggcaa      840

guaucucaga uauggcaagu gacaguaggu gcccaaccca aggcgaggcu uaucuagaua      900

aacaaaguga uacucaauac guauguaaaa ggacgcuagu agauagagga uggggaaacg      960

guuguggacu auuuggaaaa ggaagccuag uaacaugugc caaauuuaca uguucaaaga     1020

aaaugacagg aaaaaguaua caacccgaaa aucuagaaua uagaauuaug cuaaguguac     1080

auggaucuca acacucagga augauaguaa augacauagg acaugaaaca gaugaaaaua     1140

gagcaaaagu ggaaguuaca ccaaauaguc caagagcaga agcuacacug gguggauucg     1200

ggagcuuagg guuagauugu gaaccaagga caggauuaga uuucucagau cuauacuauc     1260

uaacaaugaa caacaaacau ugguuaguuc auaaagaaug guuucacgac auaccacuac     1320

cauggcacgc aggagcagau acaggaacac cacauuggaa caauaaagaa gcauuaguag     1380

aguucaaaga cgcacaugcu aaaagacaga caguaguugu ucuaggaagc caagaaggag     1440

cuguacauac agcacuugca ggagcacuug aagcagaaau ggauggugca aaagggagac     1500

uuuucagcgg acacuuaaaa ugccgaauaa aaauggauaa auuaagauua aaaggugucu     1560

cguauucuuu auguacagca gcauuuacau uuaguaaagu accagcagaa acauuacaug     1620

guacgguaac agucgaagua caauaugcag gaagagaugg accauguaaa guaccagcac     1680

agauggcagu agauaugcaa acauuaacac cuguagggag auuaauaacg gccaauccua     1740

uaauaacaga aaguacagaa aauucacgca ugaugcuuga acucgaucca ccauucggag     1800

auaguuauau agugauagga guaggugaua agaaaauaac ccaucauugg cauagaagug     1860

gaaguacuau agguaaagca uucgaagcaa cagugagagg agcuaaacga auggcaguau     1920

uaggcgauac agcuugggau uuugguagug uagguggagu auucaauagu cuaggaaaag     1980

gaguacacca aguauucgga gcggcauuua agagcuuguu cggaggaaug agcugguucu     2040

cacaaauacu uauagguaca uuauuaguau ggcuaggauu aaauacuaaa aacggaagua     2100

uuucauuagc augucuugcu cuagguggug caaugauuuu ucuaagcacu gccguuucug     2160

cagacguugg uuguagcgua gauuucugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  7050
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 2 opt

<400>  7050
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gcguaggaau aguaggauua uuauuaacga cagcaauggc ugcagaagua acucgacgcg      120

gaucagcaua uuauauguac cucgacagaa augacgcagg agaagcaauu aguuucccua      180

caacauuagg gaugaauaaa uguuauaucc aaaucaugga uuuaggacac augugugaug      240

caacaaugag cuacgaaugu ccaauguuag acgaaggugu agaaccagac gacguagacu      300

guuggugcaa uacaacauca acauggguag uauaugguac augccaucau aagaaaggcg      360

aagcuagaag aaguagacga gcaguaacac uaccaucaca uagcacaaga aaauuacaaa      420

caagaucuca aaccuggcua gaaucaagag aauauacuaa acauuugaua agaguagaaa      480

auuggauauu uagaaaucca ggauucgcuu uagcagcagc ugccauugcc uggcuuuuag      540

gaucaucaac aagccaaaaa guaauauauu uaguaaugau auugcuuaua gcgccagcau      600

acagcauaag auguauagga guuucaaaua gagauuuugu cgaaggaaug agcggaggaa      660

cauggguaga uauaguacuu gaacauggag gaugcgugac aguuauggcc caagacaaac      720

cuaccguaga cauagaauua guuacaacua cuguaucuaa uauggccgaa guaagaaguu      780

auugcuacga ggcuucaauu agugacaugg caucagauuc uagaugccca acucaaggug      840

aagcauaccu agacaaacaa agugacacgc aauacgucug uaaaagaaca uuaguagaca      900

gaggaugggg caauggaugc ggauuauucg gaaaaggcag cuuaguaacu ugugcaaaau      960

ucgcauguag caagaaaaug acaggaaaau caauacaacc agaaaacuua gaauacagaa     1020

ucauguuguc aguucauggc agccaacauu caggaaugau aguaaacgau acuggacaug     1080

aaaccgauga aaauagagca aaaguagaaa uaacuccaaa uaguccuaga gccgaagcca     1140

cauuaggugg uuuuggauca uuaggacuag auugugaacc acgaacagga cuagauuuua     1200

gugauuuaua uuaucuaacg augaauaaua aacauugguu aguacauaaa gaaugguuuc     1260

augauauacc acuuccaugg caugcaggag ccgauacugg aaccccacac uggaauaaua     1320

aagaagcauu aguugaguuc aaagaugccc acgcaaagag acaaacagua guaguauuag     1380

gaucacaaga aggggcagua cauaccgccu uagcuggagc cuuagaagca gaaauggacg     1440

gagcuaaagg aagacugagu agcggacacu ugaaaugucg acugaaaaug gauaaguuaa     1500

gguuaaaagg aguaaguuau aguuuaugua cagcugcauu uacauuuacc aaaauaccag     1560

ccgaaacccu gcaugggacg guuacaguag aaguacaaua ugcaggaacg gauggaccau     1620

guaaaguacc agcgcagaug gcaguugaua ugcaaacacu aacaccagua ggaagacuua     1680

uaaccgcaaa uccggugaua acagaaagua cagaaaacuc caaaaugaug uuagaauuag     1740

auccaccauu uggagacuca uauauaguga uaggaguggg agaaaagaaa auaacacacc     1800

auuggcauag aaguggaagu acaauaggaa aagcguucga agcaaccgua agaggagcaa     1860

aaagaauggc aguucuagga gauacugcau gggauuucgg uagcguagga ggagcauuaa     1920

auucacuagg aaaaggaauc caucaaauau uuggagcagc auucaaaagu uuauucggag     1980

gaaugaguug guuuucccaa auacuaauag gaacacuauu aaugugguua ggauugaaua     2040

cgaaaaaugg aucuauaucg cugauguguc uagcauuagg aggagucuua auuuucuuau     2100

caacagcagu aucagcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7051
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 2 opt

<400>  7051
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguuggaau agucggacuu cuuuugacaa cagcaauggc ugcugaaguu acgcgaagag      120

gaagugccua uuacauguau uuggacagaa augaugcagg agaggcuauu ucauucccaa      180

caacauuagg gaugaacaaa uguuauauac aaauaaugga ucuuggucau acaugcgacg      240

cgacgauguc auacgaaugu ccaaugcuug augaaggagu agaaccugac gauguagacu      300

guuggugcaa cacaacauca acuuggguag uauauggaac uugucaucac aaaaaagggg      360

aggcaaggag aucacguaga gcaguuacac uaccuucaca uaguacaaga aaacuacaaa      420

caagaucaca aacauggcuc gaaucacgag aauauacaaa acauuuaaua aggguagaaa      480

auuggauuuu cagaaaccca ggauuugccc uugcagcagc agcaauugca uggcuuuuag      540

gaaguagcac aagucagaaa gugauauacu uagugaugau uuuacuuaua gcuccagcau      600

auucuauaag augcauagga guaaguaaua gagacuucgu agaaggaaug agugggggua      660

cuuggguaga uguuguacuu gaacacggcg gaugcguaac aguaauggcc caagauaaac      720

caacuguaga uauagaauua guaacgacaa caguuucaaa uauggcagaa guaagaucau      780

auugcuacga agcauccaua ucagacaugg cuucugauuc uagaugucca acucaaggug      840

aagccuauuu agauaaacaa ucagauacuc aauauguaug uaaaagaacc uuaguagaca      900

gagguugggg aaauggaugc ggauuauuug gaaaaggaag ucuaguaacg ugugcaaaau      960

uugcaugcag uaaaaaaaug acuggaaaaa guauucaacc agaaaaccuu gaauauagaa     1020

uaauguugag uguacauggu agccaacauu caggaaugau aguaaaugau acaggccacg     1080

aaacagacga aaauagagca aaaguugaaa uaacaccaaa uagucccaga gccgaagcaa     1140

ccuuaggagg auucggaucu cuaggauuag acugcgaacc uagaacagga cuagacuuuu     1200

cagaucuuua cuauuuaaca augaauaaua aacauuggcu agugcauaag gaaugguuuc     1260

acgauauacc auuaccaugg caugcaggag cugauacagg uacaccacau uggaauaaua     1320

aagaagcacu uguagaauuc aaagacgcac acgcuaaacg acaaacagua guaguauuag     1380

guagucaaga aggagcaguu cauacagcau uagcuggggc auuagaagca gaaauggaug     1440

gagcaaaggg aaggcuauca ucaggacauu uaaaaugcag auuaaaaaug gauaaauuga     1500

gacucaaagg uguaucauau ucucuaugua cagcagcuuu cacuuuuaca aaaauaccag     1560

cagaaacucu ucaugggacu guuacaguug aaguacaaua ugcaggaaca gaugguccau     1620

gcaaaguacc agcacaaaug gcaguagaua ugcaaacauu aacaccagua ggaagacuga     1680

uaacagcaaa cccaguaaua acagaaagca cagaaaauuc aaaaaugaug uuagaauuag     1740

auccuccauu cggagauuca uauauaguca uaggaguagg agaaaaaaaa auaacacauc     1800

auuggcauag aucaggauca acuauaggca aagcauuuga agcaacagua agaggcgcaa     1860

aaagaauggc cguacuagga gacacagcuu gggacuucgg aagcguagga ggagcacuaa     1920

auaguuuagg aaaaggaauu caccaaauau uuggagcagc auuuaaaagu uuauucggag     1980

gaaugagcug guucucacaa auauuaauag guacuuuacu aaugugguua ggacuaaaug     2040

ccaaaaacgg aucaauaagc uuaaugugcu uagcucuugg aggugugcua auauuccuuu     2100

caacagcagu aagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7052
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 2 opt

<400>  7052
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caauuggaau aauuggucuu uuacuuacua cagcaauggc agcagaaaua acaagaagag      120

gaucagcaua uuauauguau uuagaccgua gcgaugccgg aaaagcaaua ucauucgcua      180

caacauuagg aguuaauaaa ugucauguuc aaauaaugga cuuaggacau augugugaug      240

caacuauguc uuaugaaugu ccaaugcuag acgaaggagu agaaccagau gauguagauu      300

gcugguguaa uacuacuagc acauggguug uauaugguac augucaucau aagaaaggcg      360

aagcuagaag aaguagacga gcaguaacuu uaccaucuca uucgacaaga aaauugcaga      420

cgagaucaca aacaugguua gaaaguagag aauauacuaa acauuuaauu aaaguagaaa      480

acuggauauu uagaaauccg gguuuugcau uaguggcagu cgcuauugca ugguuauuag      540

gcucaucaac gagucaaaaa guaauauauc uaguaaugau acuauugaua gccccagccu      600

auucaauaag augcauagga guaaguaaua gagauuuugu agaagguaug agcggaggca      660

cuuggguaga ugucguuuua gaacacggag gaugcguaac aguaauggcu caagacaaac      720

caaccguaga uauagaguua guuacaacaa caguaaguaa uauggcagaa guucgaucuu      780

acuguuauga agcaucaaua ucagauaugg cuagcgacuc uagaugcccc acacagggag      840

aagccuacuu agauaaacaa agugauaccc aauacguaug uaaaagaacc cuaguagaua      900

gaggaugggg caaugggugc ggacuauuug guaaaggauc ucuagugaca ugcgcaaaau      960

ucacaugcuc aaagaaaaug acaggaaaaa guauccaacc agaaaauuua gaauauagaa     1020

uaauguuauc uguacauggc ucacaacauu cagguaugau aggauaugaa acugaugaag     1080

acagagcaaa aguagaaguc acaccaaacu ccccaagagc agaagcaaca cuuggcgggu     1140

uuggaaguuu aggauuagac ugugaaccua gaacaggauu agacuucagu gauuuauauu     1200

accuaacgau gaacaauaaa cacugguuag uacauaaaga augguuucac gacauaccau     1260

uaccguggca ugcaggagca gauacuggaa caccucauug gaacaauaaa gaagcauugg     1320

ucgaguucaa agaugcucau gcaaaaagac aaaccguagu aguauuagga ucacaagaag     1380

gagcuguaca uacagcauua gcaggagcgc uagaagcaga aauggaugga gcaaaaggaa     1440

ggcuauucuc aggccaucua aaauguaggc uuaaaaugga uaaacuacga cugaaaggag     1500

uaaguuacuc auuauguaca gcagccuuca ccuucacgaa aguaccagcc gaaacucuac     1560

acggaacagu aacuguagaa guacaauaug cagguacaga uggacccugu aaaauaccag     1620

uacaaauggc aguagauaug caaacauuaa caccaguagg aagauuaauu acugcaaauc     1680

caguaauaac agaaaguaca gaaaauucaa aaaugaugcu ugaacuugau cccccauuug     1740

gagauucaua uauaguaaua ggaguaggag auaagaaaau aacacaucau uggcauagau     1800

caggaucgac aauuggaaaa gcauucgaag caacaguaag aggagcgaaa agaauggcug     1860

uauuaggaga cacagcgugg gauuuuggau cagugggagg ugucuuuaau agcuuaggaa     1920

agggaauaca ccaaauauuc ggugccgcgu uuaaaucacu cuuuggaggc augucauggu     1980

ucagucaaau acuaauagga acucuguuag uguggcuagg auuaaauacg aaaaauggaa     2040

gcauuagcuu aacauguuua gcauuaggag gagugaugau auuccuaagu acagcaguau     2100

cagcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  7053
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 2 opt

<400>  7053
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau uguuggauua uuacuaacaa cagcuauggc agcugagguc acaagaagag      120

gaucugcaua uuauauguau uuagauagga augaugcagg agaagcuaua aguuucccga      180

caacccuagg uaugaauaaa uguuauauuc aaauaaugga ucuaggacac augugugaug      240

cuacaauguc auacgaaugc ccuauguuag augaaggcgu agaaccagac gauguagauu      300

gcugguguaa uacaacgagc acauggguag uauauggaac uugccaucau aagaaaggag      360

aagcuagaag aaguaggaga gcuguaacau uaccuaguca uaguacaaga aaauuacaaa      420

caagaucgca aacaugguug gaaucaagag aauauacgaa acauuuaaua agaguagaaa      480

auuggauauu uagaaaucca ggguucgcuu uagcagcggc ugcuauugca ugguuauuag      540

ggaguagcac uucacaaaaa guaauauauu uagugaugau auuauuaaua gcuccagcau      600

auucaauaag augcauagga gugucaaaua gagauuucgu agaaggaaug ucggguggaa      660

cauggguaga uguaguuuug gaacauggcg guugcguaac aguaauggcu caagacaaac      720

cuacaguaga uauagaauua guaacaacaa caguaaguaa uauggcagaa guuagaucuu      780

auugcuauga agcaucaauu ucagauaugg caagugacuc acgaugcccc acacaagggg      840

aagcauaccu agacaaacaa ucagauacuc aauacguaug uaaaagaaca uuaguagacc      900

gggguugggg aaacggaugc ggucuauucg gaaaaggaag ucuaguaaca ugugcuaaau      960

uugcauguag uaagaaaaug acagggaaaa guauucagcc ugaaaacuua gaauauagaa     1020

uaaugcuguc uguacaugga agucaacaua gugguaugau aguaaaugau acaggacaug     1080

aaacagacga aaauagggca aaaguggaga ucacgccaaa caguccuaga gcagaagcua     1140

cacuaggagg uuuuggaagu cuagggcuag auugugaacc cagaacugga uuagauuuuu     1200

cggauuuaua cuacuuaaca augaacaaca aacacugguu gguccauaaa gaaugguuuc     1260

augauauacc auuaccaugg caugcgggcg cagauacagg aacaccacau uggaauaaca     1320

aagaagcauu agucgaauuu aaagaugccc augcaaaaag acaaaccgug guagucuuag     1380

gaagucaaga aggagcagua cauacagcac uagcuggcgc gcuugaagca gaaauggaug     1440

gagcuaaagg aaggcuaucg ucaggacauu uaaaauguag guugaaaaug gauaaacuaa     1500

ggcuaaaagg aguuaguuau agcuugugua cugcagcauu cacguuuaca aaaauaccag     1560

cagaaacauu acacgguaca guuacaguug aaguacaaua ugcagggacc gauggaccau     1620

guaaaguacc cgcacaaaug gcuguagaua ugcagacacu aacacccgua ggaagacuaa     1680

uaacagcaaa uccugucaua acagaaagua cagaaaacuc aaaaaugaug cuugaauuag     1740

auccaccauu uggagauucu uacauaguaa uaggaguagg agaaaagaaa auaacucauc     1800

acuggcaucg aaguggaagu acaauaggaa aagcauuuga agcaaccguu agaggagcaa     1860

aaagaauggc aguauuagga gacacagcuu gggauuuugg aagcguagga ggagcauuaa     1920

acucauuagg gaaagguaua caucaaauuu ucggagcagc auuuaaaagu cuauuugggg     1980

gaaugucaug guuuagucaa auauuaauag guacauugcu aaugugguua ggacuaaaua     2040

cuaaaaaugg aaguauaagu uuaauguguu uagcacuugg aggaguacua auauuuuuaa     2100

gcacagcagu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7054
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 2 opt

<400>  7054
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

caaucgguau uauaggacua cuauuaacaa cagcaauggc ggccgaaaua acgagaagag      120

gaucagcaua uuacauguau cuagaccgaa gugaugcagg aaaagcaaua ucuuuugcua      180

caacacuagg aguaaacaaa ugucauguac aaauuaugga cuuaggacau augugugacg      240

cgacaauguc auacgaaugu cccauguuag augaaggagu ugaaccagau gauguagacu      300

guugguguaa cacaaccagu acuuggguag uauacgguac augccaucac aagaaaggag      360

aagcaagaag aaguagacga gcagugacau uaccaagcca cucaacgaga aaauuacaaa      420

caagaaguca aacauggcua gaaaguagag aauacacuaa acaucuaaua aaaguagaaa      480

auuggaucuu cagaaaucca ggauuugcau uaguagcagu ugcaauugca ugguuauuag      540

gaucaucaac cagccaaaaa guuauuuauu uaguaaugau ccuauuaauu gcaccagcau      600

acaguauuag augcaucggc guuucaaaua gagauuuugu ugaagguaug ucaggaggaa      660

cauggguaga uguaguauua gaacauggug gauguguaac uguuauggca caagauaaac      720

cuacagugga cauugaauua guaacaacaa caguauccaa cauggcagaa guuagaucau      780

acugcuauga agcaagcaua agugacaugg cuucagauuc aagauguccu acacaaggag      840

aagcauaucu ggacaaacaa ucagauacuc aauaugucug caaaagaaca uuaguugaua      900

gaggcugggg aaaugggugc ggcuuauucg gaaaagguag ucuagucaca ugcgcuaaau      960

uuacauguag uaagaaaaug acaggaaaau caauccagcc agaaaacuua gaauacagaa     1020

uuauguuguc aguacaugga ucucaacaua gugguaugau uguaaacgau acaggauacg     1080

aaacagauga aaauagagcc aaaguagaag uuaccccuaa cucaccaaga gcagaagcaa     1140

cucuaggagg auuuggaucc uuaggacuag auugugaacc aagaacaggc uuagauuuuu     1200

cagaucuaua uuaccuuaca augaacaaua aacacugguu aguacauaaa gaaugguucc     1260

acgauauacc acuaccaugg cacgcaggag cggacaccgg uacaccacac uggaauaaua     1320

aagaagcauu aguugaguuc aaagaugcuc augccaaaag acaaacagua guaguuuuag     1380

gaucucaaga aggagcagua cauacagcuu uagcaggagc auuagaagca gaaauggacg     1440

gagcaaaagg aaaauuauuu ucaggacauu uaaaaugcag auuaaaaaug gacaaauuga     1500

gauuaaaagg cguaaguuau ucacuaugua cggcagcauu cacauuuacu aaagucccag     1560

ccgaaacacu gcauggaaca guuacaguag aaguucagua cgcaggcaca gauggaccau     1620

gcaaaauacc uguacaaaug gcaguagaua ugcaaacccu aacaccaguc ggaagacuua     1680

uaacugccaa uccaguuaua acagaaucaa cugaaaacuc caaaaugaug uuagaauuag     1740

auccaccauu uggagacagu uauauaguaa uuggaguagg agacaagaaa auuacacauc     1800

acuggcaucg aaguggaagu acaauaggaa aagcauuuga agcuacagua agaggagcua     1860

aaagaauggc aguacuaggu gauacagcau gggauuuugg uucuguagga gguguauuua     1920

auagucuugg aaaaggaaua caucaaauau uuggagccgc auuuaaauca uuauuuggag     1980

gaaugucaug guucucacaa auacuaauug gcacauuauu aguauggcua ggacuaaaca     2040

ccaaaaacgg aagcauaagu uuaacauguc uagcacuagg aggaguaaug auauuucuaa     2100

guacagcagu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7055
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 2 opt

<400>  7055
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caauaggaau uauuggucua cuucuaacaa cagcaauggc ugcagaaaua acaagaagag      120

gaagugcaua uuacauguau uuagacagau cagacgcugg aaaagcgauc aguuucgcaa      180

caacuuuagg aguaaauaaa ugucauguac aaauaaugga uuuaggucac augugugacg      240

caacuauguc cuaugaaugu ccaauguuag acgaaggagu ugaaccagau gauguugauu      300

guugguguaa uacaacaucu acaugggucg uauauggaac augucacagg aaaaaagggg      360

aagcaagaag aucuagaagg gcaguaaccu uaccaaguca uucaacaaga aagcuacaaa      420

caagaagcca aacauggcua gaaaguagag aauacacuaa acacuuaauu aaaguagaaa      480

auuggauauu uagaaaucca ggauucgcau uaguugcagu agcaauagca ugguuauuag      540

gcucgucaac aagucaaaaa guuaucuauu uaguaaugau cuuacuaaua gcuccagccu      600

auucaauuag gugcaucggc guaucaaaua gagacuuugu agaaggaaug agcggaggua      660

cauggguaga cguaguauua gaacauggag gaugcguaac aguuauggcu caagauaaac      720

caaccguaga uauagaacua guaacaacaa caguuaguaa uauggcagaa guuagaagcu      780

auugcuauga agcgucuaua ucagauaugg caucagauag uaggugucca acacagggag      840

aagcauacuu agacaaacaa ucggauacuc aauauguaug uaaaagaaca uuaguagaua      900

gaggcugggg uaacggaugc ggauuauucg gaaaaggauc auuagugacc ugugcuaaau      960

uuaccugcuc aaaaaaaaug acgggcaaau caauacaacc ugaaaaucua gaauaccgca     1020

uaaugcuuag uguacaugga ucccagcauu caggaaugau aguaaacgau acaggcuacg     1080

aaacagauga aaauagagca aagguagaag uuacaccuaa uucaccacga gcagaagcaa     1140

cauuaggagg auuuggaagu cuagguuuag auugugaacc aagaacagga uuagauuuuu     1200

cagaucuaua uuauuuaaca augaacaaua aacauuggcu aguacauaaa gaaugguuuc     1260

augauauacc auuaccuugg caugcaggug cagauacagg aacaccucau uggaauaaua     1320

aagaagcccu ugucgaguuc aaagaugcac acgccaaaag acaaacugua gucguacuag     1380

gaagucaaga aggugcagua cacacagcuu uagcaggagc acucgaagca gaaauggacg     1440

gagcgaaagg aaaacuguuc agcggacauu uaaaaugccg auuaaaaaug gacaaauuga     1500

gacuaaaagg aguaucauac ucuuuaugua cagcagcauu uacauuuaca aaaguuccag     1560

cagaaacauu acauggaaca guaaccguag aaguacaaua ugcaggaacu gacgguccau     1620

gcaaaauacc aguucaaaug gcaguagaca ugcaaacauu aacuccagug gguaggcuaa     1680

uuacagcaaa cccuguaaua acagaaagca cggaaaacuc uaaaaugaug uuagaacuag     1740

auccaccauu uggagauuca uacauaguaa uuggaguagg agauaaaaaa auuacacauc     1800

auuggcauag aaguggauca acaauaggaa aagcauucga agcaacugua cgaggggcaa     1860

aaagaauggc aguacuaggc gacacagcau gggauuuugg aaguguuggg gguguauuua     1920

auucuuuagg aaaaggaaua caucaaauau uuggagcggc auucaaauca uuauuuggag     1980

gaaugagcug guuuagucaa auuuuaauag guacuuugcu aguaugguua ggauuaaaua     2040

caaaaaacgg aucaauaagc cuaaccuguu uggcuuuagg aggaguaaug auauucuuaa     2100

gcacagcagu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7056
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 2 opt

<400>  7056
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcauaggaau aaucggacug uuauuaacga cagcuauggc agcugaaaua acaagaaggg      120

gauccgcgua cuacauguac uuagauagau cugaugcugg aaaagcaaua ucauucgcua      180

caacacucgg aguaaacaaa ugucauguac aaauaaugga ucuaggacac augugugaug      240

ccacaauguc cuacgaaugu ccuauguuag augaaggagu ugagccagau gauguagauu      300

gcugguguaa uacaacuucc acauggguag uauauggcac augccaucac aagaaaggag      360

aagcuagaag aaguaggaga gccguaacac uaccaagcca uaguacaagg aaauuacaaa      420

caagaaguca gacuuggcua gaaaguagag aauauacaaa acaucugaua aaaguagaaa      480

auuggauauu cagaaaucca ggauuugcuc uaguagcagu agcaauagca ugguuauuag      540

gaagcucaac aagccaaaaa guuaucuauu uaguaaugau auuauuaaua gcuccagcgu      600

auuccauaag augcauagga guaaguaaua gagacuuugu agaaggaaug agugguggga      660

cauggguaga uguugucuua gaacaugggg gauguguaac agugauggcu caagacaaac      720

caacaguuga cauagagcua guaacuacaa caguaaguaa cauggcagaa guaagaucau      780

acuguuauga agccuccaua ucagauaugg cauccgacag ucgaugucca acacaaggug      840

aagcauauuu ggacaaacaa agugauacac aguauguaug caagagaaca uuaguugaua      900

gaggaugggg uaacgggugu ggauuauuug gaaaagguuc acuggugaca ugugcaaaau      960

uuacaugcuc aaagaaaaug acuggaaaaa gcauacaacc agaaaauuua gaauauagaa     1020

uuauguuauc aguucacgga agccaacaua guggaaugac uguaaaugac auaggauaug     1080

aaacagacga aaacagagca aaaguugaag uaacgccaaa uucaccuaga gcagaagcua     1140

cauuaggagg auuuggcagu uugggauuag auugugaacc cagaacagga cuugacuuca     1200

gugauuuaua uuaucugaca augaacaaua aacauugguu aguacauaaa gagugguuuc     1260

augauauacc auuaccgugg caugcaggag cagacacagg aacuccacac uggaacaaua     1320

aagaagcucu aguagaauuu aaagacgcac acgcuaaaag acaaacagua guagucuuag     1380

ggucacaaga aggcgcagua cacacagcuu uagcaggagc auuagaagca gaaauggacg     1440

gcgccaaagg caaacuauuu ucuggccauu uaaaguguag gcuaaaaaug gacaagcuaa     1500

gauuaaaagg gguaucauau ucauuaugca cagccgccuu uacauuuaca aagguuccag     1560

cagaaacacu gcauggaaca gugacaguag aaguccaaua ugcaggaaca gauggaccau     1620

guaaaauacc aguacaaaug gccguagaca ugcaaacauu aacaccagua ggaagacuca     1680

uaacagcaaa cccugucauu acagaaagca cagaaaauuc uaaaaugaug cuagaauuag     1740

auccaccuuu uggagauucu uauaucguga uaggaguugg agauaagaaa auaacacauc     1800

auuggcauag auccggaagu acaauaggaa aagcguuuga agccacagua aggggagcaa     1860

aaaggauggc aguacucgga gauacagcgu gggauuuugg gucaguugga ggaguuuuua     1920

auagucuagg aaaagguaua caccaaauau uuggggcagc auucaaaucu uuauucggag     1980

gaaugagcug guuuucacaa auauuaauag gcacacuacu uguaugguua ggauuaaaua     2040

ccaaaaaugg aucaauaagu uugacaugcc uagcgcuagg agggguaaug auauuucugu     2100

caacagcagu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7057
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 2 opt

<400>  7057
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guauagguau aguaggccuu cuacuaacga cagcaauggc agcugaaaua acaagaagag      120

guucagcaua uuauauguau cuagauagaa gugacgcagg aaaagcuauu agcuucgcaa      180

cuacuuuagg aguuaauaaa ugucauguac aaauaaugga cuuaggacau augugcgaug      240

caacaaugag uuaugaaugc ccaauguuag augaaggagu agaaccagac gauguagauu      300

gcuggugcaa uacuacaucg acauggguag uguacggcac augucaucac aagaaaggag      360

aagcgagaag aucaagaagg gcaguaacac uaccaagcca uaguacgaga aaauuacaaa      420

cacgaucuca aacaugguua gaaucaagag aauauacaaa acacuuaaua aaagucgaga      480

acuggauauu ucggaaucca ggauucaccc ucguugcagu agccauagcu uggcuacuag      540

gauccucaac uagccaaaaa guaauuuauu uaguaaugau auuauuaaua gcuccagcau      600

auucaauaag auguauaggu guaucuaaca gagauuuugu cgaaggcaug ucaggaggaa      660

cauggguaga uguaguucua gaacauggag gaugcguaac aguuauggca caagacaaac      720

ccacuguaga uauagaauua guaacgacaa caguaucaaa uauggcagaa guacgaagcu      780

auugcuauga agccucaaua ucagacaugg cuucagauuc aagaugucca acacagggug      840

aagcauaucu cgacaaacaa ucggacacac aauauguaug uaaacgcaca uuaguagaua      900

gaggaugggg uaauggaugc ggucuauucg gaaaaggauc auuaguaaca ugugcaaaau      960

ucacuugcuc uaagaaaaug acgggaaaau caauucagcc agagaauuua gaauaccgua     1020

uaauguuauc uguucaugga ucacaacauu cugggaugau aguaaaugau gaaaauagag     1080

caaaaguaga agugacacca aauucaccaa gagcagaagc uacacuagga ggauuuggau     1140

cacuaggauu ggauugcgaa ccacgaacug gcuuagauuu uucagaucua uacuauuuaa     1200

caaugaauaa caaacauugg cuaguacaua aagaaugguu ccacgauaua ccauuaccau     1260

ggcaugcugg ggcagauacu ggaacaccac auuggaauaa uaaggaagca uuaguagaau     1320

uuaaagaugc ucacgcaaaa agacaaacag uagucguguu aggaagucaa gaaggagcag     1380

uacauaccgc auuagcuggu gcacuagaag cagaaaugga uggagcaaaa ggaagauuau     1440

uuucuggaca ucuaaaaugc cgauuaaaga uggauaaacu uagauuaaaa gguguuagcu     1500

acagucuaug uacagcagca uucacauuca ccaaaguacc ugcagagacc cuacacggaa     1560

caguuaccgu agaaguacaa uaugcaggga cugauggacc augcaaagua ccugcacaaa     1620

uggcaguaga caugcaaaca uuaaccccag uaggaagauu aauaacagcu aauccaguaa     1680

uaacagaauc aacagaaaac agcaaaauga ugcuagaauu ggacccaccu uuuggagaua     1740

guuacauagu aauaggagua ggagauaaga aaauaacaca ucacuggcau aggaguggua     1800

guacaauugg aaaagcauuu gaagcaacag uuagaggagc uaaaagaaug gcagucuuag     1860

gagauacagc augggauuuu ggaaguguag gagguguuuu uaauucguua ggaaaaggaa     1920

uacaucaaau auuuggagcc gcuuucaaaa gucuuuuugg aggaauguca ugguuuucac     1980

aaauucuuau agggacauua uuaguuuggc uuggauuaaa cacuaaaaac ggaaguauau     2040

cuuuaacaug ccuagcacua ggagggguaa ugauuuuucu aaguacagca guaagugcau     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  7058
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 2 opt

<400>  7058
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacgu       60

caauugguau aauaggccua uuacuaacaa cagcuauggc ggcagaaauu acaagaaggg      120

gaucagcaua uuauauguac cuagauagau cagaugcagg uaaagcuaua aguuuugcua      180

cuacauuggg aguaaauaaa ugucacguac aaauaaugga ucugggucau augugcgacg      240

cuacaauguc guaugaaugu ccaaugcuag acgaaggggu cgaaccugau gauguagauu      300

guugguguaa uacuacaucg acauggguag uauauggcac cugucaucac aaaaaaggag      360

aagcaagaag aucaagaagg gcaguaacac uaccaaguca uagcacuaga aaauuacaaa      420

caagaagcca aacaugguua gaaagccgag aauauacaaa acauuuaaua aaaguagaga      480

auuggauauu uagaaaucca ggauuugccu uaguagcagu ugcaauagca uggcuacuug      540

gaucauccac uagucaaaaa guaauauauc uaguaaugau auuauuaaua gcaccagcau      600

auaguauuag auguauagga guuucaaaua gagacuuugu ugaagguaug ucuggaggaa      660

cauggguaga cguagucuua gaacauggag gauguguaac aguaauggca caagauaaac      720

cuacaguaga uauagaacua guaacaacaa ccguuaguaa uauggcagaa guaaggucau      780

auuguuacga agcaucaaua ucugacaugg caagugauag ucgaugccca acacaaggug      840

aagcguauuu ggauaaacaa ucagauacac aauauguaug uaaaagaacu cuaguagaua      900

gagguugggg caacggcugu ggauuauuug gaaagggaag ccuaguaaca ugugcaaaau      960

ucacuugcag uaaaaaaaug acaggaaaau caauacaacc cgagaaccua gaauauagaa     1020

uaauguuauc aguacauggg ucgcagcacu caggaaugau aggauaugaa acagaugaaa     1080

auagggcuaa aguugagguu acgccaaauu cuccacgcgc agaagcaaca uuagguggau     1140

ucggcucacu aggacuagau ugugagccaa gaacaggauu agacuuuagu gaucuauauu     1200

aucuaacaau gaauaauaaa cauugguuag uacauaaaga augguuucau gacauaccau     1260

uaccauggca cgcaggagcu gauacaggca cuccgcauug gaauaauaaa gaagcacucg     1320

uagaguuuaa agacgcacac gccaaacgac aaacaguggu uguacuagga ucacaagaag     1380

gagcaguuca cacagcacua gcaggagcac uagaagccga aauggaugga gcuaaaggua     1440

aauuauuuag uggacaucug aaaugcagac uaaaaaugga caaacuuaga uugaaaggug     1500

uaaguuauag uuuaugcacc gcagccuuua cuuuuacaaa aguaccagca gaaacacuuc     1560

auggcacagu aacaguugaa guacaauaug cugguacaga uggaccuugu aaaauuccug     1620

uacaaauggc agucgacaug caaacauuaa caccaguugg acgccuaaua acggcuaauc     1680

caguaauaac ugaaagcaca gagaauagua aaaugauguu agaauuagau cccccauucg     1740

gagauaguua cauuguaauc ggaguuggag auaaaaaaau aacgcaucau uggcacagaa     1800

gcgguucaac aauaggaaaa gcauuugaag caacagucag aggugcgaaa agaauggcag     1860

uccucggcga uacagcaugg gauuucgguu cagugggagg aguauucaau ucucuaggga     1920

aagguauaca ucaaauauuu ggugcagccu uuaaaucauu auuuggagga augaguuggu     1980

uuucccaaau auuaauugga acauuauuag ugugguuagg gcucaacaca aaaaacggaa     2040

guauaucacu aacuuguuua gcacuaggag gaguaaugau auuuuuguca acugcuguaa     2100

gcgcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  7059
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 2 opt

<400>  7059
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggaau aguaggucua uuacuaacga cagcaauggc agcagaagua acuagaagag      120

gaucggcaua uuauauguau uuagacagaa augaugcggg ggaagcaaua ucuuuuccga      180

caacauuagg aaugaauaaa uguuauauac aaaucaugga ccuuggacau augugugacg      240

cgacaauguc auaugaaugu ccaauguugg augaaggagu agaaccagau gauguugauu      300

guugguguaa cacgacaucu acauggguag uauauggcac uugccaucau aaaaaaggag      360

aagcgagaag aagcagaaga gcagugacac uaccuaguca uucaacaaga aaauuacaaa      420

caagaucaca aacaugguua gaaucaagag aauacacaaa acacuuaauu cgaguugaaa      480

acuggauauu cagaaaccca ggauuugccu uagcugcugc agcaauagca ugguuauuag      540

gcaguaguac aagccaaaaa guaauauauc uaguaaugau ccuauuaaua gccccagcau      600

auaguauaag auguauuggu guaucuaauc gagauuuugu agaaggaaug ucaggaggaa      660

cauggguaga cguaguauua gaacacggag gaugugucac aguaauggca caagacaaac      720

caacaguaga uauugaauua gucacaacua caguaagcaa uauggcagaa guaagaaguu      780

auuguuauga agccuccaua ucagauaugg caagugacuc aagauguccg acucaaggag      840

aagcuuaucu ugauaaacaa agcgauacuc aguacguaug caaaagaaca cuuguugaua      900

gaggcugggg aaacggaugc ggauuauucg gaaaaggaag ccugguaaca ugcgcuaaau      960

uugcauguag uaaaaaaaug acaggaaaaa gcauacaacc agaaaaucua gaauaucgaa     1020

uaauguuauc aguacacggc ucacagcauu caggaaugau uguuaacgac acaggacacg     1080

aaacugacga gaauagagca aaaguugaaa uaacaccaaa cucaccgaga gcagaagcaa     1140

cauuaggagg cuucggcagc cuaggacuag acugugaacc acgaacaggc uuagacuuua     1200

gugaucuaua uuaccucacu augaauaaca aacauugguu aguucacaaa gaaugguucc     1260

augauauacc uuuaccaugg caugcaggug ccgauacugg uacaccacac uggaacaaca     1320

aagaagcauu aguagaauuu aaagaugcac augcaaaaag acaaacaguu guuguauuag     1380

gaucacaaga aggagcagua cacacagcgu uagcaggggc acuagaagca gaaauggacg     1440

gggccaaagg acggcuaagu ucaggacauu uaaaauguag auuaaaaaug gauaaauuaa     1500

gacuaaaagg aguaucguac ucgcugugca cagcagccuu uacauucaca aaaauuccug     1560

ccgaaacacu acacggaacu guaacaguag agguacaaua cgcagguacu gauggaccau     1620

guaaaguacc agcgcaaaug gcaguagaua ugcaaacauu gacaccagua ggaagauuaa     1680

uaacagcaaa uccaguaaua acagaaucua cagaaaacuc aaaaaugaug cuugaauuag     1740

auccaccuuu uggagauagu uacauaguaa uagggguagg agaaaaaaag auaacccacc     1800

auuggcauag aucaggauca accauaggga aagcauuuga agccacuguc agaggagcua     1860

aaaggauggc aguuuuagga gacacagcau gggauuucgg aagcgugggu ggcgcacuca     1920

auagucuugg acgaggaaua caucaaauau uuggugccgc auucaaaagu cuuuuuggag     1980

gaaugaguug guuuucucag auacuaauug gaacauuauu aauguggcua ggauuaaaua     2040

caaaaaaugg aaguauuuca uugauguguu uagcacuagg aggaguauua auauuucuaa     2100

guacggcagu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7060
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 2 opt

<400>  7060
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gugucggaau agugggauua cuuuuaacaa cagcaauggc ugcugaagua acuagaagag      120

guucagcaua cuacauguac uuagacagaa augaugcagg agaagcaaua aguuuuccga      180

caacauuggg aaugaauaaa uguuauauac aaauaaugga uuuaggacau augugugaug      240

caacaauguc auaugaaugu cccauguuag acgaaggcgu agaaccagau gauguggauu      300

gcuggugcaa uacaacauca acuuggguag ucuacggaac augucaucac aagaaaggug      360

aagcaagaag aagcagacga gcaguaacau uaccuaguca uagcacuaga aaguuacaaa      420

cacggaguca aacuugguua gagaguaggg aauauacaaa gcauuuaaua agaguagaaa      480

acuggauauu cagaaaucca ggucuagcuc uagcugcagc ugcaauugca uggcuauuag      540

gaucaaguac cucacaaaaa gugauauauu uaguaaugau uuuacuaauc gcaccagcau      600

auucaauaag augcauagga guuagcaauc gagauuucgu agaaggaaug aguggaggaa      660

cauggguaga uguuguauua gaacacggug gauguguuac agugauggcg caagauaaac      720

cuacaguaga uauagaauua guuacaacaa cuguuagcaa uauggcggaa guaagaaguu      780

auuguuacga agccaguaua agugauaugg caucggacag uagaugcccc acacaaggug      840

aagcauaucu agauaaacaa ucagauacac aauauguuug caaaagaaca uuaguagaca      900

gagguugggg aaaugguugc ggucuuuuug gaaaagguag ucuaguaaca ugcgcaaaau      960

ucgccuguag caagaaaaug acagguaaaa guauacagcc ugaaaacuua gaguauagaa     1020

uaaugcuauc aguacacggg agccaacaca gcggaaugau uguuaacgac acgggacaug     1080

aaacagauga aaacagagca aaaguagaaa uuacaccgaa uagcccucga gcagaagcca     1140

cacuaggugg uuuuggaagc cuaggauugg acugugaacc aaggacaggu cuagauuuua     1200

gugaucuaua uuacuuaaca augaacaaua aacauugguu aguacauaaa gaaugguuuc     1260

augacauccc uuuaccaugg caugcaggag cagauaccgg aacuccacau uggaacaaua     1320

aagaagcauu aguagaauuu aaagaugcac acgcaaagag acaaacagua guaguauuag     1380

gaagccaaga aggagccgua cauacagccu uagcaggcgc guuagaagca gaaauggaug     1440

gagcaaaggg uagauuaagu ucgggacauu ugaaauguag gcucaaaaug gauaaauuac     1500

gauugaaagg aguaaguuac ucuuugugua cagcagcuuu uacuuuuaca aaaauaccag     1560

cagaaacauu acaugguaca gucacugugg aaguacaaua ugcaggaaca gauggaccau     1620

gcaaaguacc ugcacaaaug gcaguagaca ugcaaacacu uacaccggua gguagauuaa     1680

uaacagcuaa uccaguaaua accgaaagua cagaaaacag caaaaugaug uuagaauuag     1740

acccgccauu uggagauuca uauauaguaa uuggaguagg agaaaagaaa auaacacacc     1800

auuggcauag aucaggaagu acuauaggua aagcguucga agcuacagua agaggagcaa     1860

aaagaauggc cguguugggc gacacugcau gggacuuugg aucgguugga ggugcacuaa     1920

auucauuagg uaaaggcauu caccaaauau uuggagcagc auuuaaguca uuauuuggag     1980

gaaugagcug guuuucacaa auacuaauug gaacguuauu aauguggcua ggauuaaaua     2040

cuaaaaaugg uagcauuagu uuaauguguc uagccuuggg aggaguguua aucuuccuua     2100

guacagcagu uagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7061
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 2 opt

<400>  7061
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguagguau cguaggauua cuauuaacaa cagcaauggc agcagaaguu acgagaagag      120

guucagccua cuauauguau cuugauagga acgaugcagg ggaagcuauc aguuuuccua      180

caacauuagg aaugaacaaa ugcuauauuc aaauaaugga uuuaggacau augugugacg      240

cuacuaugag cuaugaaugu ccuaugcuag acgaaggcgu agaaccugau gacguagacu      300

guugguguaa uacaacaucg accuggguag uguauggaac gugucaucau aagaaaggag      360

aagcaagaag aagcagacga gcuguaacau uaccaucaca uaguacaaga aaauuacaaa      420

cacgaucaca aacauggcua gaaaguagag aauauacaaa acauuuaauu agaguugaaa      480

auuggauauu uaggaaucca gguuuugcau uggcagcagc ugcaauugcg uggcugcucg      540

gcaguucaac aucacaaaaa guaauauauc uaguaaugau auugcuaaua gcuccagcuu      600

auucaauaag augcauagga guuucaaaua gagacuuugu ugaaggcaug aguggaggca      660

cauggguaga cguaguauua gaacacggag gaugcgugac uguaauggca caagauaaac      720

caacaguaga uauugaguug guaacaacaa cagugucaaa uauggcagaa guaagaaguu      780

auuguuacga agcuagcaua agugauaugg caucagacag cagauguccu acucaaggcg      840

aagcauaucu cgacaaacaa agugauacac aauacguaug uaaaaggaca cuggucgaca      900

gagggugggg aaacggcugu gguuuguuug gaaaaggaag ucuaguaacc ugugcaaaau      960

uugcuuguag uaaaaagaug acgggaaaga guauccaacc agaaaacuug gaauauagaa     1020

uaauguuauc cgugcacggg agucaacauu caggaaugau aguaaacgau acaggacaug     1080

aaacagacga aaauagagca aaaguggaaa uuacuccaaa uaguccaagg gccgaagcua     1140

cucuuggcgg auuuggauca uuaggauuag acugcgaacc gaggacagga cuagauuuca     1200

gugauuugua cuaucuaaca augaacaaca aacauugguu aguccauaaa gaaugguucc     1260

augauauucc auuaccgugg caugcuggug cggauacugg aacaccacau uggaauaaua     1320

aagaagcauu aguagaauuu aaagaugcuc augcaaaaag acaaaccgua guaguuuuag     1380

guagucaaga aggagcagua cacacagcau uggcgggagc ccuagaagca gaaauggaug     1440

gagcaaaagg gagacuaucu ucaggacacu ugaaauguag auugaaaaug gauaaacuca     1500

gauuaaaagg gguaucauau ucauuaugua cagcugcauu cacauuuaca aaaauaccag     1560

cugaaacacu acaugguacu guuacgguag aaguacaaua ugcaggcaca gacggaccuu     1620

guaaaguucc agcacaaaug gcaguagaca ugcaaacauu aacaccugua ggaagauuaa     1680

uaacagcaaa uccagugaua acagaaucaa cagaaaauag uaaaaugaug uuagaauuag     1740

auccaccauu uggcgauuca uauauaguaa uagguguagg agaaaagaaa aucacccacc     1800

auugguauag aagcggaagc acaauaggua aagcauuuga agcaacagua agaggagcaa     1860

aaaggauggc cguucuagga gacacugcau gggauuuugg aaguguagga ggagcacuaa     1920

acucuuuagg aaaagguauu caucaaauau uuggagcagc auuuaaaucg uuauucggag     1980

gaaugagcug guuuucacaa aucuuaauag gaacauuacu aauguggcuu ggauugaaua     2040

ccaaaaaugg aucgauaucu uuaauguguc uagcacuagg gggaguuuua auauuucuau     2100

caacagcagu gagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7062
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 2 opt

<400>  7062
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacgu       60

cuguuggaau aguuggauua uuacuaacaa cagcaauggc agcagaaguu acaagaagag      120

gcucagccua uuacauguac uuagauagaa augacgcagg agaagcuauu agcuuuccga      180

cuacucuagg aaugaauaaa ugcuacauac aaauaaugga cuuaggacac augugcgaug      240

caacaaugag uuacgaaugu ccaaugcuag acgagggugu ugaaccggau gauguagauu      300

guugguguaa caccacaagc acuugggugg uauauggaac gugucaccac aaaaaaggag      360

aagcacguag aucacgaagg gcaguaacac ucccaucaca uaguacuaga aaauuacaaa      420

caagaaguca aacgugguua gaaucaagag aauacaccaa acacuuaauu agaguagaaa      480

auuggauauu ucgaaaucca gguuuugcuc uagcagcagc agcaauagca ugguuauuag      540

gaagcucgac aagucaaaaa guaauauauu uaguaaugau acuauuaaua gcuccggcuu      600

auucaauaag auguauagga guaucaaaua gagacuuugu agaaggaaug ucuggaggaa      660

cuuggguuga ugugguauua gaacaugggg gauguguaac aguaauggcc caagauaaac      720

caacaguuga uauagaacuu guuacaacaa ccguaaguaa cauggccgaa guaagauccu      780

auugcuauga agcaucaaua ucggauaugg caagugauuc aagauguccu acucaaggag      840

aagccuacuu agacaaacaa agcgauacac aauauguaug caaaaggaca cuaguagauc      900

gugguugggg aaacggaugu ggauuguucg gaaaagguag ccucguaaca ugugcaaaau      960

uugcuuguag uaaaaaaaug acaggaaaau caauacaacc cgaaaauuua gaauauagaa     1020

uaaugcuaag uguccacggg agccaacaua gcgguaugau aguuaacgau acaggacaug     1080

aaacugauga aaauagggca aaagucgaaa uaacaccaaa uaguccaaga gcagaagcaa     1140

cauuaggugg auuuggaucu uuaggacuag acugugaacc aaggacuggu uuagauuuua     1200

gcgacuuaua uuaucuaaca augaacaaua aacauugguu aguacauaaa gaaugguucc     1260

augauauacc auuaccaugg cacgcaggag cagauaccgg uaccccgcac uggaauaaua     1320

aagaagcacu aguugaauuu aaagacgcac augcaaaacg ucagacagua guaguauuag     1380

gaagucaaga aggcgcagua cauacagcau uagcaggagc acuugaagcg gaaauggaug     1440

gugcaaaagg aagauuaagu ucaggacauu ugaagugucg guuaaaaaug gacaaacuaa     1500

gauuaaaagg aguaucauau ucgcuaugua cagcugcuuu uacauucacu aaaaucccag     1560

cugaaacacu acauggaaca guaacaguag aaguucaaua ugcagguacu gacggaccau     1620

gcaaaguccc agcacaaaug gcaguggaua cacagaccuu aacacccgug ggaagacuua     1680

uuacugcaaa uccaguaaua acugaaucaa cugaaaauuc aaaaaugaug uuagaauuag     1740

auccaccauu uggagacucg uauaucguaa uugguguagg agaaaaaaaa auaacucauc     1800

acuggcauag aucaggauca acaauaggaa aagcauucga agcuacagua agaggcgcaa     1860

aaagaauggc aguccuagga gauacugcau gggacuucgg aagcguagga ggcgcacuua     1920

auucucuagg uaaaggaaua caucaaauau uuggagcagc auuuaaaagc uuauuuggag     1980

gaauguccug guuuucacaa auacuaauag gaacuuuguu aauguggcuu ggucuuaaua     2040

caaaaaaugg gucaauaagu uuaaugugcc uagcuuuagg aggggucuua auauuucuuu     2100

ccacugcagu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7063
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 2 opt

<400>  7063
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguuggcau uguaggauua cuauuaacaa cagcuauggc agcagaagua acacgaaggg      120

gaucagcaua uuauauguau cuagauagaa acgaugcagg agaagcaauc agcuucccaa      180

caacacuagg gaugaauaaa uguuauauac aaauuaugga uuugggccac augugugacg      240

caacaaugag uuaugaaugc ccaaugcuug acgaaggagu ugaacccgau gauguagacu      300

gcugguguaa uacaaccuca acuuggguag uuuauggaac cugucaucac aagaaaggag      360

aagcucgaag aucaagacga gcaguaacau uaccuaguca uucaacgaga aaauuacaaa      420

cuagaucaca aacuuggcuu gaauccagag aauacacaaa acaucuaaua agaguugaaa      480

acuggaucuu uagaaacccu gguuuugccu uagcagcggc ugcaauagca ugguuacuag      540

gaucaucaac aagucagaaa gucauauauu uagucaugau auuacuaaua gcuccugcau      600

acucuauaag auguauaggu gugaguaaua gagacuuugu ggaaggaaug ucugguggaa      660

caugggugga cguaguuuua gagcacgggg gaugcguuac aguaauggcc caagauaaac      720

caacaguuga uauugagcua guaacaacga ccguaagcaa uauggcagaa auaagaucau      780

auugcuauga agccucaauu agugacaugg cuagcgauag cagaugccca acacagggag      840

aagcauaccu ggacaaacaa ucagacacac aauauguaug caaaagaaca uuaguagaca      900

gaggaugggg aaaugguugu ggacuauuug gaaaaggguc auuaguaaca ugcgcaaaau      960

uugcauguuc aaagaaaaug accgguaaau caauacaacc agaaaauuua gaauacagaa     1020

uaaugcuauc aguacacggu agucaacaua gcgggaugau uguaaacgau acaggacacg     1080

aaacagauga aaaucgcgca aaaguagaaa uaacgccaaa uaguccuaga gcugaagcaa     1140

cauugggugg auuugggagu uuaggacuag auugcgaacc uagaacagga cuagauuuua     1200

gugacuuaua uuaucucaca augaauaaua aacacugguu aguucacaaa gaaugguuuc     1260

augauauacc auuaccaugg caugcaggag cagauacagg uacaccccau uggaacaaua     1320

aagaagcucu aguagaguuu aaagacgcac augcaaaacg acaaacagua guaguuuuag     1380

guagucaaga aggcgcagug cauacagcac uagcaggagc auuagaagca gaaauggacg     1440

gugcgaaagg ucgauuaagu aguggacauu uaaaaugccg acuaaaaaug gacaaauuac     1500

gauuaaaagg uguaucauac ucauuaugua ccgcugcauu cacuuuuaca aaaauaccug     1560

cagaaacauu gcacggaacu guaacaguag aaguacaaua ugcggguaca gauggaccau     1620

gcaaaguucc agcccaaaug gcuguggaua ugcaaacacu aacaccagua gguagacuua     1680

uaacggcaaa uccaguaaua acagaaagua cagaaaauuc aaaaaugaug uuagaauuag     1740

aucccccauu uggagacagc uauauaguua uaggaguagg agagaaaaag auaacucauc     1800

auuggcaucg aagcgguuca acaauuggaa aagcauuuga agcaacugua agaggagcaa     1860

aaagaauggc cgucuuaggg gacacugcuu gggauuucgg aucaguaggc ggugcauuaa     1920

auucacuagg aaaagguauu caccaaauau uuggagcagc auucaaauca uuauucggag     1980

gcaugaguug guucagucaa auacuaauag gaacauuacu aauguggcuu ggacuaaaca     2040

cuaaaaaugg uucaauaagu uuaauguguc uugcauuagg cggcguacuu auauuuuuau     2100

caacagcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7064
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 2 opt

<400>  7064
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

caguagguau aguuggauua cuacuaacaa ccgcaauggc ugcagaagua acaagaagag      120

gaucagcaua uuauauguau uuagacagaa acgaugcagg ggaagcgaua ucuuuuccaa      180

caacuuuagg gaugaauaaa uguuacauac aaauaaugga ccuaggucac augugugaug      240

caacuauguc auaugaaugc ccaaugcucg augaaggagu agaaccagac gacguagacu      300

guugguguaa uacaacuuca acauggguag uauauggcac augccaucau aagaaaggag      360

aagcaagaag aucacguaga gcaguaacau ugccaucaca uucuaccaga aaacuacaaa      420

caagaagcca aacaugguua gaaucaagag aauacacaaa acacuuaaua agaguagaaa      480

auuggauuuu uagaaaccca gguuucgcuu uagcugcagc agcuauagcu uggcuauuag      540

ggaguagcac aucacaaaaa gucauauauc uaguuaugau ccuccugaua gcaccagcau      600

auaguauacg auguauagga guaucaaaca gggacuucgu ugaaggaaug aguggaggaa      660

cuuggguaga uguuguucua gagcauggag gcuguguaac uguaauggca caagacaaac      720

caacaguaga cauagagcua guaacaacua cuguaagcaa uauggcugaa guaagaucuu      780

auugcuauga agcaagcaua ucagauaugg caagugacag ucgaugucca acccaaggag      840

aggcuuauuu agauaaacaa uccgauacuc aauauguuug uaaacgaacc cuaguggaca      900

gaggaugggg uaacggaugu ggacuauuug ggaaaggaag ccucgugaca ugugcaaaau      960

uugcuuguuc gaaaaagaug acaggaaaau caauacaacc ugaaaaucua gaauauagaa     1020

uaaugcuauc uguacacgga agccaacaua guggaaugau aguaaaugac acaggucaug     1080

aaacagacga gaacagagca aaaguugaaa uuacgcccaa uagcccucga gcagaagcua     1140

cacuaggggg auuugguucu cuaggacuag acugugaacc aagaacaggc cuagauuuuu     1200

cagaccuaua uuauuuaaca augaauaaua aacacuggcu aguccauaaa gaaugguucc     1260

acgauauacc auuacccugg cacgccggag cagacacagg aacaccucac uggaauaaua     1320

aagaagcccu uguagaauuu aaagaugcac acgcaaaacg ucaaaccgua guaguauuag     1380

gcagucaaga aggagcaguu cauacagcau uagcuggagc auuagaagca gaaauggacg     1440

gagcaaaggg cagacuaucc ucaggacauc uaaaaugcag auugaaaaug gauaaacuac     1500

gauuaaaagg aguaaguuau ucacugugua cagcagcauu uacguuuaca aaaauaccag     1560

cagaaacauu acacggaaca gucacaguag aaguucaaua ugcaggaaca gacgguccau     1620

guaaaguauu agcacaaaug gcuguagaua ugcaaacacu aacaccugua ggaagauuaa     1680

uuacugcaaa uccuguuauc acugaaagca cagaaaacuc aaaaaugaug cuugaauuag     1740

auccaccauu uggcgauuca uacaucguca uuggaguagg agaaaagaaa auaacgcauc     1800

acuggcaccg aaguggauca acaauaggua aagcauuuga agcaacagua cguggagcua     1860

aaagaauggc aguuuuagga gauacagcuu gggauuuugg aagugucggu ggagcacuaa     1920

acucacuagg aaaaggaaua caucaaauau uuggagcagc auucaaauca uuauuuggag     1980

gcaugucaug guuuagucaa auuuuaauag gaacucuacu aaugugguua ggauuaaaca     2040

cuaaaaacgg uucaauaucc uuaauguguc uagccuuagg aggaguucuu auauuucuca     2100

gcacagcugu aagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7065
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 2 opt

<400>  7065
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

guguaggaau aguaggauug uugcuaacaa cagcuauggc ugcagaagua acuagaagag      120

gcagugccua cuacauguac uuagacagaa augacgcugg ugaagcaauu agcuuuccaa      180

caacacuagg aaugaacaaa uguuauauac aaauaaugga cuuaggccau augugugaug      240

caaccauguc auacgagugu ccaacauuag acgagggcgu ugaaccagac gacguagauu      300

gcugguguaa uaccacuuca acauggguag uauaugguac uugucaccau aagaaaggug      360

aagcaagaag aucacguaga gcagugaccu uaccuucaca uucuacaaga aaauuacaaa      420

caaggaguca aacauggcuu gaaucaagag aguauacaaa acaccuaaua aggguugaaa      480

auuggauauu ucgaaacccg ggguuugcac uagccgcagc ugcaauagca uggcuccuag      540

gaaguucaac uagccaaaaa guaauauacc uaguaaugau cuugcuuaua gccccagcau      600

acaguauaag auguauugga gugaguaaua gagauuucgu agaaggaaug ucaggaggga      660

cuuggguuga uguaguauua gaacauggag gaugcguaac cguaauggca caagauaaac      720

cgacgguuga uauagaauua guaaccacaa caguaaguaa uauggcugag guaagaaguu      780

acuguuauga ggcaucaaua ucagacaugg ccagcgacag ucguugucca acgcaaggag      840

aagccuauuu agacaaacaa ucagauacac aauauguaug uaaaagaacu cuaguugaua      900

gaggaugggg aaacggcugc ggacuuuuug gaaaaggauc auuagugaca ugcgcaaaau      960

uugccuguag uaagaaaaug acagguaaau cuauacaacc ugaaaauuua gaauauagaa     1020

uuauguuauc aguacacgga ucacaacauu cggggaugau aguaaaugau accggacacg     1080

aaacagauga aaauagagcu aaaguugaaa ucacaccaaa caguccaaga gcagaagcaa     1140

cauuaggcgg auucggauca cuaggccuag auugugaacc aagaacagga cuagauuuua     1200

gcgaucuuua uuacuuaaca augaauaaua aacauugguu aguucauaaa gaaugguuuc     1260

augauauacc ccuuccaugg caugccggag cagauacugg aacaccacac uggaauaaca     1320

aagaagcauu agucgaauuu aaggaugcac augcaaaaag acaaacuguu guaguauuag     1380

gaucacaaga aggagcagua cauacagcuu uagcuggagc acuagaagcu gaaauggacg     1440

gagcaaaagg acgauuaagc ucuggacacc uaaaauguag acuaaaaaug gauaaacuaa     1500

gacucaaagg aguuucuuau ucguuaugua cugcagcauu uacuuuuaca aaaauuccag     1560

cagaaacgcu acauggaaca guaacuguag aaguacaaua ugccggaacu gauggaccau     1620

guaaaguccc agcacaaaug gcaguugaua ugcaaacacu aacuccagua ggaagauuaa     1680

uaaccgcuaa uccagucaua acagaaucaa cagaaaauuc aaaaaugaug uuagaauugg     1740

auccucccuu uggugacagu uacauaguaa uaggaguagg agaaaagaaa auaacacauc     1800

acuggcauag aaguggauca acuauaggua aagcauuuga agcuacagua cgaggagcaa     1860

aaagaauggc cguucuugga gacacagcuu gggacuuugg aaguguagga ggagcucuaa     1920

auagcuuagg aaaggguaua caucaaauau uuggagccgc uuuuaaaagu cuauucggag     1980

gaaugucaug guuuucacaa auauuaauag gaacacuacu aaugugguua ggacuaaaua     2040

cgaaaaaugg cagcauuagc uuaauguguu uagcacuagg aggaguucua auauucuuau     2100

caacagccgu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7066
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 2 opt

<400>  7066
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau agugggauua cuuuuaacaa cggcaauggc agcugaagua acaagaagag      120

gcagugcuua uuauauguau uuagacagga augaugcagg ugaagcaauu aguuuuccua      180

caacuuuagg caugaacaaa uguuacauac aaauaaugga ucuaggacau augugcgaug      240

caacaauguc auaugagugc ccuauguuag augaaggugu ugaaccagac gauguggauu      300

gcugguguaa uaccaccagc acuuggguag uauacggaac augucaucau aaaaaaggcg      360

aagcaagaag aaguaggaga gcuguaacuc ucccuaguca uucuacgaga aaauuacaaa      420

caagaucaca aacuuggcuu gaaucaagag aauauacaaa acauuuaaua cgaguagaaa      480

acuggauauu uagaaaucca gguuucgcac uggcagcagc agcaauagca ugguuacuag      540

gaucaucaac aagccaaaaa guaauauacu uaguaaugau ucuguuaaua gcaccagcgu      600

auagcauccg uuguauaggu guaucaaaua gagauuuugu cgaaggaaug aguggaggaa      660

cauggguuga uguaguucua gaacacggag gcugcgucac agucauggca caagauaaac      720

caacgguaga uauugaauua guaacaacaa ccgucagcaa cauggcagaa guaagaucau      780

acuguuauga agcaucaaua uccgauaugg caucagacuc uagaugucca acucagggag      840

aggcauauuu agauaaacaa ucagauacgc aauauguaug uaaaagaaca uuaguagaua      900

gagggugggg caauggaugu ggauuauuug gaaagggauc uuuaguuacu ugugcaaaau      960

uugcaugcuc aaaaaaaaug acaggaaaaa guauucaacc agaaaauuua gaauaccgaa     1020

uaaugcuuag uguacacgga agucaacauu caggaaugau cgucaaugac acagggcacg     1080

aaacagacga aaacagagca aaaguagaaa uaacaccuaa uucuccaaga gcagaagcaa     1140

cuuuaggugg auucggaagc uugggccuug auugugaacc aagaaccggg cuugauuuua     1200

gcgauuuaua cuauuuaaca augaauaaua aacauugguu aguacauaaa gaaugguucc     1260

augauauacc ucuaccaugg cacgcaggug cagauacagg aacaccacac uggaacaaua     1320

aagaagccuu aguugaauuu aaagaugcac augcgaaaag acaaacaguc gucguacuag     1380

gaagucaaga aggagcagua cauacugcuc uugcaggggc cuuagaagca gagauggaug     1440

gagcaaaagg aagauuaucu ucgggacauc ucaaauguag auuaaaaaug gauaaauuaa     1500

gacuaaaagg ugucuccuac agucuaugua cggcagcauu cacauuuaca aaaauaccug     1560

cagaaaccuu acauggaaca guaacagucg aaguacaaua ugcaggaaca gaugguccau     1620

guaaaguacc agcacaaaug gcaguugaua ugcagacauu aacacccgua ggaagauuaa     1680

uaacagcaaa uccagucaua acugaaucaa cagaaaacag uaaaaugaug uuagaauuag     1740

auccgccauu cggagauagu uauauaguua uuggagucgg ugaaaaaaaa auaacucacc     1800

auuggcacag aucaggauca acaauaggaa aagcauuuga agcaacagua agaggagcaa     1860

aaagaauggc aguguuagga gauacugcau gggauuuugg aaguguagga ggagccuuga     1920

auucauuagg uaaagguaua caccaaauau uuggggccgc auuuaaaagc uuauuuggag     1980

gaaugaguug guuuucacaa auucuaauag gaacauuauu aauguggcuu ggauuaaaua     2040

cuaaaaacgg uucuauuuca cuaauguguu uagcauuagg cgggguucua auauuucuuu     2100

cuacggucgu uucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7067
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 2 opt

<400>  7067
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguugguau aguuggacua cuauuaacaa cagcgauggc agcugaagua acaagaagag      120

gaucagcaua uuacauguac uuagauagga acgaugcugg ugaagcaauu ucauuuccua      180

caacacuagg aaugaauaaa uguuauauac aaauaaugga ccuaggucau augugcgaug      240

cuacuauguc uuaugaaugc ccaaugcuag augaaggagu agaaccagau gauguagacu      300

guugguguaa cacaaccagu gcauggguag uauaugguac cugccaucac aagaaaggag      360

aagcuagaag aucuagacga gcuguaacau uaccuagcca uucaacgaga aaauuacaga      420

caagaccaca aacuuggcua gaaucuagag aauacaccaa acaucuaaua aggguagaaa      480

auuggauauu uagaaaucca gguuuugcac uagcugccgc agcaauugcu ugguuacuag      540

guucaucaac aucucaaaaa guaaucuauc uaguuaugau cuuacuaaua gcuccugcau      600

auaguauaag augcauagga guaagcaaca gagauuuugu agaagguaug ucagguggaa      660

cauggguuga uguaguacuu gaacacggug gaugugugac uguaauggca caggauaaac      720

ccacagugga cauagaauua guaacaacaa cugugucaaa uauggcugaa guaagaucau      780

acugcuauga agcaucaauu agugauaugg cauccgauag uagauguccu acacaaggag      840

aagcauaucu agauaaacaa agugacacac aauaugucug uaaaagaacg uuaguugaua      900

gaggaugggg aaauggaugc ggauuauuug gaaaaggauc acuuguaacc ugugcaaaau      960

uugcauguuc aaagaaaaug acaggcaaaa guauacaacc agaaaaucua gaauauagaa     1020

uaauguuauc gguccaugga ucacaacaua gcgguaugau uguaaaugau acaggacacg     1080

aaacagacga aaauagggca aaaguugaaa ucaccccaaa cucacccaga gcagaagcaa     1140

cucugggagg auuuggcagc cugggauuag auugugaacc aagaacugga cuugauuuua     1200

gugaucuaua cuaucuuaca augaacaaua aacauuggcu cguacauaaa gaaugguucc     1260

augauauacc auuaccuugg caugcaggag ccgauacagg aacaccacac uggaacaaua     1320

aagaagcauu aguagaguuc aaagacgcac augcaaaaag gcaaacagua guuguacuag     1380

gaagccaaga aggagcaguu cauacugcau uagcgggugc auuagaagcc gaaauggaug     1440

gagcgaaagg uagauuaucu ucuggacacu ugaaaugucg cuuaaaaaug gauaaacuaa     1500

gacuaaaagg aguaaguuac ucauuaugca cagcagcauu cacauuuaca aaaauuccug     1560

cggaaacacu acacggaacu guaacaguag aaguacaaua ugcuggaacu gauggaccau     1620

guaaaguucc cgcacaaaug gcaguugaca ugcaaacauu aacaccggua ggaagauuga     1680

uuacugcgaa cccuguuaua acagaaagua cggaaaauag uaaaaugaug cuagaauuag     1740

auccaccuuu uggagauagu uauauaguaa uaggaguagg ugaaaagaaa auaacacauc     1800

auuggcacag aagcggauca acaauaggaa aagcauuuga agcuacaguu agaggagcua     1860

aacgaauggc cguacuugga gacacagccu gggauuuugg cuccguagga ggagcauuaa     1920

acagucuagg uaaaggcaua caucaaauau uuggagcagc cuuuaaaagu uuauuuggag     1980

guaugucaug guuuucacaa auacuaauag gaacauuauu aauguggcua ggacuaaaca     2040

cuaaaaaugg aucgauaagc cuuaugugcu uagcacuagg agguguuuua auauuucuaa     2100

gcacagcagu gucagcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7068
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 2 opt

<400>  7068
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cugugggaau aguagguuua uuauuaacua ccgcaauggc ugccgaagua acaagaagag      120

gaucagcaua uuauauguac cuugauagaa augaugcagg agaagcaaua ucuuuuccaa      180

caacauuagg uaugaauaaa ugcuacauac aaauuaugga uuuaggacau augugugaug      240

caacgauguc auaugaaugu ccaaugcuug augaaggagu ugaaccugac gauguagauu      300

guugguguaa uacaacaagc accuggguug uauauggaac augucaucau aaaaaaggag      360

aagcucgaag aaguagaaga gcaguaaccc uaccgagcca cagcacuaga aaauuacaaa      420

cuaggucaca aaccuggcua gaaaguagag aauacacaaa acaucuuaua agaguagaaa      480

auuggaucuu uagaaaucca gguuucgcac uagcagcagc agcaauagca uggcuacuag      540

guucguccac aucaaaaaaa guaauauauu uaguaaugau auuacuaaua gcaccagcau      600

auucaauaag auguauaggu guaaguaaua gagauuucgu ugaaggaaug aguggaggaa      660

cauggguaga uguagucuua gaacacggag gaugugugac uguuauggca caagacaaac      720

caacaguaga cauagaacuu guaacgacaa caguaagcaa uauggcagaa guacguaguu      780

auugcuauga agcaucaauu ucagauaugg caucagauuc cagaugucca acacaaggag      840

aagcuuauuu agauaaacag ucagauacgc aauauguuug caaacgaacc cuaguugaca      900

gagggugggg caacggaugc ggauuguucg gaaaaggauc guuaguaacu ugugccaaau      960

ucgcauguuc uaaaaaaaug accggaaaau caauacaacc agaaaauuua gaauauagaa     1020

ucaugcuaag cgugcauggc ucacaacacu cuggcaugau uguaaaugau acaggacaug     1080

aaacugacga gaauagagcu aaaguagaaa uaacaccgaa uucacccaga gcagaagcaa     1140

cauuaggagg auucggauca cugggauuag auugcgaacc acgaacagga uuagauuuca     1200

gugaccuuua uuauuuaaca augaauaaua aacauugguu aguacauaaa gaaugguucc     1260

acgacauacc auuaccuugg caugcaggcg ccgauacagg aacaccacau uggaauaaua     1320

aagaagcucu aguagaauuu aaagaugcac augcaaaaag gcaaacagua gucguucuag     1380

gaucacaaga aggagcagua cauacagcau uagcaggagc cuuagaagcc gaaauggaug     1440

gcgcaaaagg aagacuaagc agcggacauu ugaaaugucg guuaaagaug gacaaguuaa     1500

gguuaaaagg cguuagcuac aguuuaugua cagcagcauu uaccuucaca aaaaucccug     1560

ccgaaacauu acauggaaca guuacaguag aaguucaaua ugccgggacu gacggaccau     1620

guaaaguacc agcucaaaug gcaguagaca ugcaaacauu aacaccuguu ggaagacuaa     1680

uaacagcaaa uccagugaua acugagagua cagaaaacag uaaaaugaug uuagaauugg     1740

acccuccauu cggugacagc uauaucguaa uaggaguagg ugaaaaaaaa auaacacauc     1800

acuggcacag aucaggauca acaauaggua aagcauucga agcaacagua cguggagcaa     1860

aacgaauggc gguucuagga gauacugcgu gggauuucgg aagcgucgga ggggcauuaa     1920

acucauuagg gaaaggaauu caucaaauau uuggugccgc auuuaaaagc cuauuuggag     1980

guaugaguug guuuagucaa auauuaauug gaacacuccu uauguggcuc ggauuaaaua     2040

cuaaaaacgg aucuauuagu uuaauguguu uggcauuagg ugguguacuu auauuuuuaa     2100

guacagcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7069
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 2 opt

<400>  7069
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

guguagguau aguaggauua uuacuaacca cagcuauggc cgcagaagua accagaagag      120

gaagugcaua cuauauguac uuagaucgga augaugcagg ggaagccaua agcuuuccaa      180

caacucuagg uaugaauaaa ugcuauauac aaauaaugga cuuaggacau augugugaug      240

cgacaaugag cuaugaaugu ccaaugcuag acgaaggcgu agaaccagau gauguugacu      300

gcugguguaa cacaacaagu acauggguag uauacgggac augucaccau aaaaaaggcg      360

aagcgcgaag aucaagaaga gcaguuacau uaccaaguca uuccacuaga aaauuacaaa      420

cccguucaca aacauggcuu gaaucacgag aauacacaaa acaucuuauu cguguagaaa      480

acuggauauu ccgaaauccg ggauuugcac uagcagcagc agcaaucgca ugguuauuag      540

gaaguucuac aagacaaaaa guuauauacc ucguaaugau acuuuuaaua gcaccagccu      600

auagcauaag auguauagga guauccaaca gagauuucgu ugaaggaaug aguggaggaa      660

cauggguaga cguagucuua gaacauggag gauguguaac aguaauggcu caagauaaac      720

cuacaguuga uauagaacuc guaacaacua caguaaguaa uauggccgaa guccguucau      780

auuguuauga agcaucaaua ucagauaugg cgagcgacag uagauguccu acccaaggag      840

aagcauauuu agacaaacaa agugauacac aauaugucug caaaagaaca uuaguggaca      900

gagguugggg uaauggaugc ggacuauucg gaaaaggaag uuuaguaaca ugugccaaau      960

uugccuguuc aaagaaaaug acaggaaaau caauacaacc agaaaacuua gaauauagaa     1020

uuauguuauc aguccauggc ucacaacaca gugggaugau agucaacgac acaggacaug     1080

aaacagauga aaauagagcu aaaguagaaa uuacaccaaa uucaccccga gcggaagcaa     1140

cacuaggagg auuuggauca cuaggauuag auugugaacc uagaacaggu uuagacuuua     1200

gugauuuaua cuaucuuaca augaacaaua aacacugguu aguacacaaa gagugguucc     1260

augacauacc acuuccaugg caugcaggag cagacacggg gacgccacau uggaauaaua     1320

aagaagcauu aguagaauuu aaagaugcac augcgaaaag acaaacggua guaguacuag     1380

guagccaaga aggagcagua cacacagccu uagcaggugc auuagaagca gaaauggacg     1440

gagcuaaagg acgguuauca agugggcacu uaaaaugcag acuaaaaaug gacaaauuaa     1500

gauuaaaagg aguaagcuac ucauuaugca cagcagcauu uacauucacu aaaauccccg     1560

cagagacuuu acauggcaca gugaccguag aaguacaaua cgcaggcaca gauggaccau     1620

guaaaguucc ugcacaaaug gcuguagaua ugcaaacguu aacaccaguu gguagauuaa     1680

uaacugcaaa uccuguaauu accgagucca cggaaaauuc aaaaaugaug cuagaauuag     1740

acccaccauu uggagauucu uauauaguaa uaggagucgg agagaagaaa auaacucauc     1800

auuggcauag aagcggaagu acaauaggaa aagcauuuga agcaacaguc agaggagcca     1860

aaagaauggc aguucuagga gacaccgcuu gggauuuugg aucaguagga ggugccuuaa     1920

auucucuagg aaaaggaaua caucagauau uuggugcagc auuuaaaagu uuauucggug     1980

gaaugucgug guuuucacag aucuuaauag gaacgcugcu aauguggcua ggacuaaaua     2040

caaaaaaugg aucaauauca uuaauguguc uagcauuagg uggcguacua auauuuuuau     2100

caacagcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7070
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 2 opt

<400>  7070
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gugucggcau aguaggucuc uuacugacaa cagcgauggc agcagaagua acaagaagag      120

gcagugcaua cuacauguau uuagauagga augaugccgg ggaagcaaua ucauucccaa      180

caacuuuagg uaugaauaaa uguuauauac aaauaaugga uuuggggcau augugugacg      240

cuaccaugag uuacgaaugu ccaaugcuug augaaggugu cgaaccagau gauguagauu      300

guugguguaa uacaacuagu acauggguag uuuauggaac uugucaucac aaaaaaggag      360

aagcgagaag aagcagaaga gcugugacac uccccuccca uaguacuaga aaacuacaaa      420

cacgaagcca aacaugguua gaaucaagag aauauacaaa acauuuaaua agaguagaaa      480

auuggauuuu uagaaaucca ggauuugcau uagcugcagc ugcaauagca uggcuccugg      540

guucaucaac uagucagaaa gugauuuauu uaguuaugau acuacuaauc gcacccgcgu      600

auucaauaag guguauaggu guauccaaca gagauuucgu agaaggaaug ucuggaggaa      660

cauggguuga uguaguuuua gaacacggug gguguguuac aguaauggcu caagacaaac      720

caacuguaga uauugaacua guaacaacua cuguaagcaa uauggcagaa gucagaaguu      780

auuguuacga agccucaaua agcgacaugg cuucagauuc uagaugucca acacaagggg      840

aagcauauuu agauaaacaa ucagauacac aauaugucug caaaagaaca cuagucgaca      900

gaggaugggg gaacgguugc ggguuauucg gcaaaggauc auuaguuacc ugcgcaaaau      960

ucgcauguag caaaaaaaug acggguaaaa guauacaacc agaaaaucuu gaauauagaa     1020

uaaugcuauc uguucacggu ucacaacaua guggaaugau agucaaugau acaggacaug     1080

aaaccgauga aaacagagca aaaguugaaa ucacaccaaa uucaccucga gcagaggcua     1140

cauuaggagg cuuugguagu cuuggauuag acugugaacc gagaacagga uuagauuuua     1200

gugauuuaua uuauuuaaca augaauaaua aacauuggcu gguacacaaa gaaugguuuc     1260

augauauucc acuuccaugg cacgcaggug cagauacagg uacaccgcac uggaauaaca     1320

aagaagcauu agucgaauuu aaagacgcac augcaaaaag acaaacagua guuguuuuag     1380

gcacucaaga aggugcaguc cauacggcac uagcgggggc acuagaagca gaaauggaug     1440

gugcuaaagg aagacuaagu ucaggacauu uaaaauguag auuaaaaaug gauaaauuaa     1500

gacuaaaagg aguaucauau ucauuaugua cagcagcauu uacauuuacg aaaauuccag     1560

ccgaaacccu acacgguacu guuacuguag aaguccaaua ugcagguaca gauggaccau     1620

guaaaguacc ugcgcaaaug gcaguagaua ugcaaacacu uacaccagua ggaagauuaa     1680

uaacugcgaa uccaguaaua acggaaucca cagaaaauuc aaagaugaug uuagaauuag     1740

auccaccuuu uggagauuca uacauaguaa ucggaguugg ugaaaaaaaa auaacacauc     1800

auuggcauag aucaggcuca acaauaggaa aagccuuuga ggcaacagua agaggcgcca     1860

aaagaauggc aguccuaggu gacacagcau gggauuuugg aucuguagga ggagcauuaa     1920

auucccuagg aaaaggaaua caccaaauuu uuggagccgc uuuuaaaucc uuauuuggag     1980

gaaugucaug guuuucacaa auucuaauug gaacacuauu gaugugguua ggcuuaaaca     2040

caaaaaacgg aagcaucagu uuaauguguc uggcacucgg aggaguacua auauuccuau     2100

caacagcagu uagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7071
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 2 opt

<400>  7071
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacuu       60

cuguaggaau aguaggccua uuguuaacaa cugccauggc cgcagaaguc acaagaaggg      120

gcagcgcaua cuauauguau uuagauagaa acgaugcagg agaggcaauu ucauuuccaa      180

caacacuagg aaugaauaaa uguuauauac aaauaaugga ucuuggucau augugugacg      240

caacaauguc guaugaaugu ccuaugcuug acgaaggugu ugaaccagau gauguagauu      300

guuggugcaa uacaacauca acuuggguag uauacggaac cugucaucau aaaaaaggag      360

aggcaagaag aucaagaaga gcaguuacgc uaccuaguca uucgacaagg aaauuacaaa      420

caagaaguca aacauggcua gaaagcagag aauacacuaa acaccuaaua agaguagaaa      480

acuggauauu uagaaauccu ggauuugcac uagcagcugc ggcaauagca uggcuauugg      540

gcucaucaac aagccaaaaa guaauauauc uagucaugau acucuuaaua gcaccagcau      600

acagcuuaag auguauagga gucagcaauc gagauuuugu agaggguaug aguggaggua      660

caugggugga uguaguucuu gaacauggag gguguguaac aguaauggcu caagauaaac      720

caacaguaga uauagaguug guaacgacaa cagucagcaa uauggcagaa guaagaagcu      780

acuguuacga agcaucaaua ucagauaugg caagugacuc aagaugucca acacaaggag      840

aagcauauuu agauaaacaa uccgauacuc aauauguaug uaaacgaacg uuaguagaca      900

gagguugggg aaauggaugu ggccuuuuug gaaaagguuc acuaguaacu ugugccaaau      960

uugcauguuc aaaaaaaaug acaggaaaau caauacaacc agaaaauuua gaauauagaa     1020

ucaugcuuuc aguccaugga agucagcacu caggaaugau cgucaaugau acaggacaug     1080

aaacugauga aaauagagcu aaaguugaaa uuacaccgaa uuccccaagg gcugaagcaa     1140

cucuuggcgg auuugguagu cugggucuag acugcgaacc aagaacaggu uuagacuuuu     1200

cagauuuaua cuaccuaaca augaauaaua aacauugguu agugcauaaa gaaugguuuc     1260

acgauauucc auuaccaugg cacgcaggug cagauacagg uacaccacau uggaauaaua     1320

aagaggcacu aguagaauuu aaagaugcac acgcaaaaag acaaacagua guaguauuag     1380

guucacaaga aggagcagua cauacagcuu uagcaggagc auuagaagcc gaaauggacg     1440

gcgccaaagg aagacuuuca agcgggcauu uaaaaugcag auuaaaaaug gauaaacuua     1500

ggcuaaaagg aguaaguuau aguuuaugua cagccgcuuu cacauuuacu aaaauaccag     1560

cagaaacacu acacgguaca guuacuguug aaguucaaua cgcagguaca gauggucccu     1620

guaaaguacc agcacaaaug gcuguagaca ugcaaacgcu uaccccagua ggaagauuaa     1680

uuacugcaaa cccaguaaua acagaaucaa cagagaacag caaaaugaug uuagaauuag     1740

auccaccuuu cggugauuca uauauaguaa uaggaguagg agaaaaaaaa auaacacauc     1800

auuggcauag aagugggagu acaauuggua aagcguucga agcuacuguu agaggagcaa     1860

aacgaauggc gguauuagga gauacagccu gggauuucgg aaguguuggu ggagcauuaa     1920

auaguuuagg caaaggaaua caucaaauau ucggagcugc auuuaagagu cuauucggug     1980

guaugaguug guucucacaa auacuaauug gaacacuauu aaugugguua ggauuaaaua     2040

caaaaaacgg aaguauauca cuaauguguu uagcauuagg uggaguuuua auuuuucuaa     2100

gcacagcagu aucagcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7072
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 2 opt

<400>  7072
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

guguuggcau uguaggguua uuauuaacaa cggcuauggc agcagaagua acaagaagag      120

gaucagcaua uuauauguau uuagauagaa acgacgcagg agaagcaaua ucuuucccaa      180

caacacuagg uaugaauaaa uguuauauac aaauaaugga ucuaggucau augugugaug      240

cuacaaugag cuaugaaugc ccaaugcuag augaaggagu agaaccggau gacguagacu      300

guugguguaa uacaacaucu acauggguag uauacggaac augucaccau aagaaaggag      360

aagcaagaag aucgcguaga gcagucacac uccccucaca uucaaccaga aaauuacaaa      420

cuagaaguca aacuugguua gaaucaagag aauauacuaa acacuuaaua agaauagaaa      480

acuggauauu cagaaaucca ggauuugcau uagcugcggc agcuauagca uggcucuuag      540

gaucuucuac cagucaaaaa guaauauacc uaguaaugau acucuuaaua gcaccagcau      600

auucuauacg augcauugga gugaguaaca gagauuuugu ugaaggaaug ucaggaggaa      660

cauggguaga cguuguucuc gaacacggag gauguguaac cgugauggcu caggauaaac      720

caacaguaga uauugaauua guaacaacaa caguuucaaa uauggcagaa guccgaaguu      780

acuguuacga agcauccaua ucagacaugg caucggauuc aagaugucca acccaaggag      840

aagccuauuu agauaaacag ucagacacuc aauauguuug uaaacgaacc uuaguagauc      900

gaggcugggg uaauggaugu ggacuuuucg guaaaggcag ucuuguaacc ugcgcaaaau      960

ucgcauguag uaagaaaaug acaggaaaaa guauccaacc agaaaaccug gaauauagaa     1020

uuaugcuauc aguccacgga ucacaacauu caggaaugau aguaaaugau acuggucaug     1080

aaacagauga aaauagggca aaaguagaaa uaacuccaaa uucaccacga gccgaggcca     1140

cccuuggagg auuuggauca uuaggccuag auugcgaacc uagaacagga uuagauuuua     1200

gugauuuaua cuauuuaacc augaacaaca aacauugguu aguucauaaa gaaugguuuc     1260

augauauacc auuaccaugg caugcuggag cagauacagg uacaccacau uggaacaaua     1320

aagaagcauu aguugaauuu aaagaugcac acgcuaaacg ucaaacuguu guuguuuuag     1380

gaucacagga aggagcugua cauacagcau uagcgggugc cuuagaagca gaaauggacg     1440

gagcaaaagg ucgacuaagu aguggucacu uaaaauguag auuaaaaaug gacaaauuaa     1500

gauuaaaagg uguuuccuau uccuuaugua cagcagcguu uaccuucacc aaaauaccag     1560

cagaaacccu acacggaaca guaacaguag aaguacaaua ugcagguaca gauggacccu     1620

guaaaguucc ugcacaaaug gcaguagaca ugcaaacuuu aaccccagua ggaagauuaa     1680

uaacagccaa uccagugauu acagaaucca cagaaaacuc gaaaaugaug uuagaauugg     1740

auccaccauu cggugauagu uacauaguua uaggaguagg agaaaagaaa auuacacacc     1800

auuggcacag aagcggaagc acaauaggaa aagcauucga agcaacagua agaggagcaa     1860

aaagaauggc ugucuuagga gauacagcau gggauuucgg uucuguuggc ggagcuuuga     1920

acaguuuggg caaaggaaua caucaaauau uuggggcagc auuuaagagc uuauuuggag     1980

guaugucaug guucucacaa auauuaauag gaacauuacu aauguggcua ggauuaaaua     2040

cgaaaaaugg cagcauuuca cuaauguguc uagcauuagg uggagucuua auauuucuau     2100

caacagcagu aucagcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7073
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 2 opt

<400>  7073
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cagugggaau ugugggauua uuauuaacaa ccgcuauggc agcagaaguc acuagaagag      120

gaucugcaua uuacauguau cuagauagaa acgaugcagg agaagcaaua aguuucccaa      180

caacauuagg uaugaauaaa uguuacauac agauaaugga ucuaggucau augugcgaug      240

caacuaugag uuaugaaugc ccaauguuag augaaggagu agaaccagau gauguagauu      300

guugguguaa cacaacauca acauggguag ucuacggaac augucaucau aagaaaggug      360

aagcuagaag aagccgcagg gcaguaacuu uaccgucgca cucaacaaga aaacuucaga      420

caagaaguca aacuugguua gaaaguagag aauauacaaa acauuuaauc cguguagaaa      480

auuggauauu aaggaaccca ggauucgcau uagcagcagc ggcaauagcc ugguuacuag      540

guucaagcac gucacaaaaa guaauauacu uagucaugau acuguuaauu gcaccagcau      600

auagcauucg uuguauuggc guaucuaaca gagauuucgu ugaaggaaug ucgggaggaa      660

cauggguuga uguaguucua gaacauggcg guuguguaac aguaauggcc caagauaaac      720

caacaguaga uaucgaacuc guaacuacaa cgguauccaa uauggcagaa guucgaaguu      780

auuguuacga agcaaguaua agugacaugg ccuccgauuc aagauguccu acgcagggug      840

aggcuuaucu agacaaacaa ucggacacac aauauguaug uaaaagaaca uuaguagaua      900

gaggaugggg aaauggaugu ggacuauuug gaaaaggaag uuuaguaacc ugugcaaaau      960

uugcuugcag uaagaaaaug acagguaaaa gcauacaacc agaaaaucuu gaauauagaa     1020

uaaugcugag cguacacggc agccaacacu cuggaaugau uguaaacgau acugggcaug     1080

aaacagauga aaaucgagca aaaguagaaa uaacaccaaa cagcccaagg gcugaagcaa     1140

cccugggugg auuuggaagu uugggucuag auugcgaacc aagaaccggu cuagauuucu     1200

cagauuuaua uuauuuaacg augaauaaua aacauugguu aguacauaaa gaaugguucc     1260

augauauacc auuaccaugg caugcaggag cugauacagg aacaccacau uggaauaaca     1320

aagaagcauu aguagaguuc aaagaugcac acgcaaaaag acaaacugua guaguacuag     1380

gaagucaaga aggagcaguc cacacagcau uagcaggagc ucuagaagca gaaauggaug     1440

gugcaaaagg ccgacuaucu ucuggacauu uaaaauguag auuaaaaaug gacaaacuaa     1500

gauuaaaagg agugucauac ucauuaugca cagcagcauu uacauuuaca aaaauuccag     1560

cagaaacacu acauggaaca guaacuguag aaguccaaua ugccggaaca gacggaccuu     1620

guaaaguacc agcacaaaug gccguagaua ugcaaacauu aacaccugua gguagacuaa     1680

uuacagccaa uccaguaaua acugaaucca cugaaaauag caaaaugaug cuagaacugg     1740

auccuccauu uggagauuca uauauaguca uagggauagg agagaaaaag auaacacacc     1800

acuggcauag aucuggaagu acuauaggua aagcauucga agcaacagua agaggugcaa     1860

aaaggauggc aguauuagga gacacagccu gggauuucgg aucaguagga ggugcauuaa     1920

auucauuagg aaaagguauc caccaaauuu ucggagcagc uuuuaaaucu uuauucggug     1980

gaaugaguug guuuucacaa auauuaauug gaacuuuacu aauguggcua ggauuaaaua     2040

ccaaaaaugg aucaauaagu cuuauguguc uagcauuagg uggaguacuu auauuucuaa     2100

guacagcagu aucagcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7074
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 2 opt

<400>  7074
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacuu       60

caguaggaau cgucggacuu uuguuaacaa cggccauggc ugcagaggua acaagaagag      120

gaucagcuua uuacauguau uuagauagga augaugcugg agaagccaua aguuucccaa      180

cuacacuagg aaugaauaaa uguuauauuc aaauaaugga uuuaggucau augugugacg      240

ccacuaugag uuacgagugu ccaauguugg augaaggcgu agaaccagac gauguugacu      300

guuggugcaa caccacauca acauggguag uauaugguac augucaucau aagaaaggag      360

aagcaagaag aucaagaagg gcaguaacac ucccuucaca uucaacaaga aaauuacaaa      420

cgagaagcca gacauggcua gagucaagag aauauacuaa acacuuaaua agaguagaaa      480

auuggauauu cagaaaucca ggguuugcau uagcagcagc agcaauagcu ugguuacuag      540

gaaguaguac gucacaaaaa guaauauauc uuguaaugau uuuauuaauu gcaccagcuu      600

auagcauuag auguauaggc guuuccaacc gugauuuugu ugaaggaaug ucuggaggaa      660

cauggguuga uguagugcua gaacacggag gauguguaac aguuauggca caagauaaac      720

caacagugga cauagagcua guaucaacua cugucagcaa uauggcggaa guaagaucau      780

acuguuacga agccacaaua agugauauag caucagacuc aagaugccca acacaaggag      840

aagcauaucu agacaaacaa ucagacacuc aauauguaug uaaaagaaca uugguagaua      900

gaggaugggg aaauggaugc ggauuauuug gaaaaggcag uuuaguuaca ugugcaaaau      960

uugccugcag uaagaaaaug acuggaaaau caauacaacc ugaaaauuua gaauauagaa     1020

uaauguuauc aguacauggg ucucagcacu caggaaugau agucaaugac acaggacacg     1080

aaacugauga aaauagagcu aaaguugaaa uaacaccaaa uagcccacga gcagaagcaa     1140

cauuaggagg cuuuggaagu uuaggacuag auugugaacc aagaacaggc cuagacuuuu     1200

cagaucuaua uuauuuaaca augaacaaca aacauuggcu aguacacaaa gaaugguucc     1260

acgauauacc auugccaugg cacgcaggag cagacacagg aacuccacau uggaauaaua     1320

aagaagcucu uguagaauuu aaagaugcac augcaaaaag acaaacagcc guaguacuag     1380

guucacaaga aggagcugua cacacagcac uagcuggagc auuagaggca gaaauggaug     1440

gagcuaaagg aagauugucg agugggcauc uaaaaugcag auuaaaaaug gacaaauuac     1500

gauuaaaagg aguaucuuau ucacuaugca ccgccgcauu uacauuuaca aaaauaccgg     1560

cagaaaccuu acauggaacc guaacaguug aaguacaaua ugcaggaaca gaugguccau     1620

guaaaguacc agcucaaaug gcaguugaua ugcaaacauu aacaccaguc ggaagacuua     1680

uaacagcaaa uccaguaaua acagaaucca cagaaaauag caaaaugaug cuggaacuag     1740

auccuccauu uggggacuca uacaucguaa uaggaguagg agaaaagaaa auaacgcacc     1800

auuggcauag aagugguagu acaauaggaa aagcauucga agcuacagua agaggagcga     1860

aaagaauggc aguauuaggu gacacugcuu gggauuuugg aucagucggc ggggcauuaa     1920

auucauuagg uaaaggaaua caucagauuu uuggagcagc cuuuaaauca uuauuuggug     1980

ggaugaguug guuuucacaa auauuaauag gaaccuuacu uaugugguua ggcuuaaaua     2040

cuaaaaaugg uagcauauca cuuaugugcu uagcauuagg uggagucuua auauuuuuau     2100

cuacagcagu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7075
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 2 opt

<400>  7075
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguagguau aguugguuua cuuuuaacaa cagcaauggc agcagaagua acuagaagag      120

gaagugcaua uuauauguac cuagaucgua acgacgcggg ugaagcaauc agcuuuccca      180

ccacuuuagg aaugaauaaa uguuacauac aaauaaugga cuuaggacau augugugaug      240

caacaaugag uuaugaaugu ccaaugcuag augagggagu ugaaccagau gaugucgacu      300

guugguguaa uacaacaagu acuuggguug uauaugggac uugucaucau aagaaaggug      360

aagcuaggag aucaagacgc gcagugacac uaccuaguca uagcacuaga aaacuccaaa      420

cacgaucaca aacaugguua gaaucacgcg aauacacaaa acacuugaua cguguagaaa      480

acuggauauu ucguaaccca gguuucgcac uggcagcagc agcaauagca uggcuauuag      540

gaaguagcac uucacaaaaa gucauauauc uagucaugau cuuauuaaua gcaccagcau      600

auaguauaag augcauaggg guaucaaaca gagauuucgu agaaggaaug aguggaggaa      660

cauggguaga cguaguacug gaacacggag gaugcguaac uguaauggca caagacaaac      720

cuacaguaga uauagaguua guaacgacua caguuaguaa cauggcagaa guuagaaguu      780

auuguuauga agcaaguaua agugauaugg caagcgauag uagaugccca acccaaggag      840

aagcauauuu agauaaacag agcgacacuc aauauguuug uaaaagaacu uuaguagaca      900

ggggaugggg gaacggaugu ggacuauuug gaaaaggauc cuugguuaca ugugcaaaau      960

uugcauguuc aaagaaaaug acaggaaaau caauccagcc ugaaaaccuu gaauacagaa     1020

ucaugcuaag cguacauggg ucacaacacu caggaaugau aguaaacgau acgggacaug     1080

aaacagauga aaacagagca aaaguggaaa uaaccccaaa uagcccacga gcagaagcaa     1140

cucuuggagg uuuuggaucu cuaggauuag auugugaacc ccgaacuggu cuagauuuua     1200

gugacuuaua uuaucucacu augaauaaca aacacugguu aguacauaaa gaaugguucc     1260

augauauacc acuacccugg caugcuggag cagacacggg aacaccacau uggaauaaua     1320

aagaagcucu aguagaauuu aaagaugcac acgcaaaaag acagacagua gugguauuag     1380

gaucacaaga aggugcagua cauacagcac uugcaggagc ccuagaagca gaaauggacg     1440

gagcaaaagg ccgacuaagu aguggacauc uaaaauguag auuaaaaaug gacaaauuaa     1500

gacuaaaagg aguaucuuau ucauuaugua cagcggcauu uacauuuacu aaaauacccg     1560

cugaaacacu acacggaacc guaacaguag aacuacaaua ugcagguaca gaugguccau     1620

guaaaguacc ugcccaaaug gcuguagaua ugcaaacguu aacaccaguu ggaagguuaa     1680

uaaccgcaaa cccaguaaua acagaaucaa cggaaaacag caaaaugaug uuagaauuag     1740

acccaccauu uggugacuca uauauaguaa ucggaguagg agaaaagaaa auaacacauc     1800

acuggcauag aaguggaagu acuauaggaa aagcauuuga agcaacagua agaggagcaa     1860

aaagaauggc cguauuagga gauacugcuu gggauuuugg uucaguaggu ggagcacuaa     1920

acagucuagg aaaaggaaua caccaaauau uuggugcagc auuuaaaagu cuauucggcg     1980

gaaugucgug guuuucacaa auacuaauag guacacuauu aauguggcuu ggacuuaaua     2040

cuaaaaaugg aagcaucagc uuaauguguu uagcauuagg aggcguccua auauucuuaa     2100

guacagcagu aagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7076
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 2 opt

<400>  7076
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggcau aguaggauua cuauuaacaa cagcaauggc ugcagaagua acaaggcgag      120

gaagugcaua uuauauguau uuagauagaa augacgcagg cgaagcaaua aguuuuccaa      180

caacauuagg aaugaacaaa uguuacauac aaauaaugga uuuaggacau augugugacg      240

caacaauguc auaugaaugu ccaauguuag augaaggagu ggaaccagau gauguagacu      300

guuggugcaa uacuacaagu acguggguag uauacggaac gugccaucau aaaaaaggag      360

aagcccgaag aagccgaaga gcuguaacuc uacccagcca uucuacccgc aaauuacaaa      420

ccagaucaca aaccugguua gaaucaagag aauauacaaa acacuuaauu agaguugaga      480

auuggaucuu uagaaauccc gguuuugcau uagcagcagc ggcaaucgca uggcuauuag      540

gaucuucaac aucacaaaaa guaauauacu uaguaaugau auuauuaaua gcuccagcau      600

acaguauaag auguauagga guaucaaaua gagauuucgu ugaaggaaug ucuggaggaa      660

cauggguaga uguaguacua gaacacggcg gauguguuac aguaauggca caagauaaac      720

cuacaguaga uauagagcuc gucacaacua ccguuucaaa uauggcagaa guaaggucau      780

auugcuacga agcaagcauc agugauaugg caagcgauuc aagaugucca acucaaggag      840

aagcauaucu agacaaacaa ucagacacac aauauguaug caaaagaaca cuuguagaca      900

ggggaugggg uaaugggugc ggccuauucg guaaagguuc gcuaguaaca ugcgcgaaau      960

uugcaugcag uaaaaaaaug acagguaaaa guauucaacc agaaaacuua gaauauagga     1020

uaaugcuauc uguucaugga ucacaacaca gcggaaugau aguaaaugau acuggacacg     1080

aaacagauga aaaccgagca aaaguagaaa uaacaccaaa uagcccaaga gcugaagcaa     1140

cucuaggugg auuugguuca cuagguuugg acugugaacc aagaacagga cuggauuuuu     1200

cagaucuaua uuauuugacc augaacaaca aacauuggcu uguacauaaa gaaugguucc     1260

augauauccc auuaccaugg caugcaggcg cggauacagg aacaccacau uggaauaaca     1320

aagaagcacu aguggaguuc aaagacgcac acgcaaaaag acagacagua guaguacuag     1380

gaucacaaga aggagcaguu cauacagcuu uagccggcgc uuuggaagca gaaauggaug     1440

gagcaaaagg uagacuuagu uccggccauc uuaaaugucg acugaaaaug gauaaacuac     1500

gacuaaaagg agugucauau ucacucugua cagcagcauu uacuuucacu aaaauaccag     1560

cagaaacauu acauggaacu guaacaguag agguacaaua ugcuggcaca gacggcccau     1620

guaaaguacc agcacaaaug gcugucgaua ugcagaccuu aacuccuguu gguagacuaa     1680

uaaccgcaaa uccaguaauu acagaaagua uagaaaauag caaaaugaug cuugaacuag     1740

auccaccauu uggugauucg uauauaguua uagggguugg agaaaaaaaa auaacucauc     1800

auuggcauag aucaggauca acuauaggaa aagcauuuga agcuacagua agaggagcaa     1860

aaaggauggc aguuuuaggu gacacagcau gggauuuugg uucuguagga ggagcauuaa     1920

acucccuagg uaaaggaaua caucaaauau uuggugcagc cuuuaaaagc cuauuuggag     1980

guaugucuug guuuucacaa auauuaauag gaacacuucu aauguggcua ggacuaaaca     2040

cuaagaaugg aaguaucagc uuaauguguu uagcacucgg aggaguacua auauuuuuau     2100

caacagcagu guccgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7077
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 2 opt

<400>  7077
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguuggaau aguaggucua uuacuaacaa cagcaauggc ugcagaagua acucggcgug      120

gaagcgcuua uuacauguac uuagacagaa augaugcagg cgaagcaaua ucauuuccaa      180

caacacuagg caugaauaaa ugcuacauac aaauaaugga uuuagggcau augugugaug      240

caacuauguc uuacgaaugu ccaauguuag augaaggcgu agaaccagau gauguagauu      300

guuggugcaa uacaacuuca acauggguag uauacgguac augucaucac aagaaaggag      360

aagcuagaag aucaaggaga gcgguaacac uaccuaguca uaguacaaga aaacuacaaa      420

cucgaucaca aaccugguua gaaucacgug aauacacaaa acauuuaaua aggguagaaa      480

auuggauauu cagaaaucca gguuuugcac uggcagcagc ugcaauagca uggcuuuuag      540

gaucaagcac aagucaaaaa gucauauacc uggugaugau auuauuaaua gcuccagcuu      600

auaguauacg auguauagga guaaguaaua gagauuucgu agaaggaaug ucaggaggua      660

caugggucga ugucguucua gaacauggag gaugugucac gguaauggca caagacaaac      720

caacaguaga uauugaauug guaacuacaa cuguaagcaa cauggcagaa guaagaucau      780

auuguuacga agcaucuaua agugacaugg caucagauuc acgaugccca acacaaggug      840

aagcauauuu agauaaacaa ucugacacuc aauauguaug uaaaagaacu cuaguagauc      900

gaggaugggg aaaugguugu gggcuauuug gaaaaggaag ccuaguaaca ugcgcaaaau      960

uugccuguuc aaagaaaaug acaggaaaau caauccaacc agaaaaccuc gaauauagaa     1020

uaaugcuauc aguacacgga ucacaacaca gcggaaugau aguaaaugac acaggacaug     1080

aaacagacga aaacagagca aaaguagaaa uaacacccaa uagccccaga gcugaagcaa     1140

cauuaggagg auucggaagu uuaggacuug acugugaacc aagaacagga uuagacuuca     1200

gcgaccuaua uuauuuaaca augaauaaca aacauuggcu aguacacaaa gaaugguuuc     1260

acgauauacc uuuaccaugg cacgcaggcg cugacacugg uacuccacac uggaauaaca     1320

aagaagcacu uguagaauuu aaagaugcgc augcaaaaag acaaacagua gugguacuag     1380

gcucucaaga aggugcagua cauacagcac uagcaggcgc auuagaagcc gaaauggaug     1440

gcguaaaagg aagacuauca ucggggcauu uaaaaugucg ucuaaaaaug gacaaauuaa     1500

gacuaaaagg aguaucguau ucauuaugca ccgcagcauu cacauuuaca aaaauaccag     1560

cagaaacauu acauggaaca guaacaguag aaguacaaua ugcagguaca gacggaccau     1620

guaaaguucc agcacaaaug gcaguggaca ugcaaacauu aacaccggua ggaagauuaa     1680

uaacugcaaa uccaguuauc acggaaagua cagaaaauuc caaaaugaug uuagaguuag     1740

acccuccauu uggagauagu uauauaguua uugguguugg ggaaaagaaa auaacucauc     1800

acuggcauag aucaggcagu acgauaggaa aagcauuuga agcuacagua cgaggcgcca     1860

aaagaauggc aguauuagga gauacagcau gggacuuugg uagcguaggu ggagcccuaa     1920

auucauuggg caaagguauu caucaaauau uuggagcugc auuuaaaagc cucuucggug     1980

gaaugaguug guuuucucaa auacuaauag gaacauuauu aaugugguua ggacuaaaua     2040

cuaaaaaugg uucuauuagc uuuauguguu uggcacuagg aggagucuug auauuucuaa     2100

guaccgcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7078
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 2 opt

<400>  7078
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cuguugguau cguaggauua uuacucacaa cagcaauggc ggccgaagua acuagaaggg      120

gaucagcaua uuauauguau cuagauagaa acgaugcagg agaagccaua aguuuuccga      180

caacauuagg caugaacaaa ugcuauauac aaauaaugga uuuagggcau augugugaug      240

caaccaugag cuaugaaugc ccaauguuag acgaaggagu agaaccagac gacguagauu      300

guugguguaa cacgacaagu acaugggucg uauacggaac uugucaucau aaaaaaggag      360

aagcuaggcg uucaagaaga gcaguaacau uaccaucaca uucaacacga aaacuucaaa      420

caagaucaca aacauggcua gaaucaagag aauauacuaa acauuuaaua aggguugaaa      480

acuggauauu uagaaaccca ggauuugccc uagcugcagc agcaaucgca uggcuacuag      540

gaucaaguac aagccaaaaa guaauauauc uaguaaugau acuauuaaua gcuccagcau      600

acucgauuag augcauaggu guaucaaaua gagacuucgu agaaggaaug uccggaggaa      660

cauggguaga cguaguguua gaacauggag gauguguuac aguaauggca caggacaaac      720

caaccguuga cauagaauua guuacaacaa caguuaguaa cauggcugag guacguucau      780

auuguuauga agcaucaaua ucagacaugg caucagauuc acgauguccc acucaaggug      840

aagcauacuu agauaaacaa agcgacacac aauacguuug uaaacgaaca cuaguagaua      900

gaggaugggg gaauggaugu ggccuauuug gaaaaggaag ccuaguaaca ugugcuaaau      960

uugcauguag uaaaaaaaug acaggaaaaa gcauucaacc ggaaaacuua gaauauagaa     1020

uuauguuauc aguacauggu ucacaacauu cagggaugau aguaaaugau accggacaug     1080

aaacugacga aaacagagca aaaguagaaa uaacuccuaa uaguccaaga gcagaagcca     1140

cauuaggggg auuugggagu cuaggacuag auugcgaacc uagaacagga uuagauuuuu     1200

cagaucuaua uuauuuaaca augaacaaua aacacuggcu uguucauaaa gaaugguuuc     1260

acgacauacc acuaccaugg caugcaggag cagauacagg aacaccacau uggaacaaua     1320

aagaagcauu aguggaauuu aaagaugcac augcaaaaag gcaaacuguc guaguucuag     1380

guucccaaga aggugcugua cauacugcac uagcaggggc acucgaagca gaaauggacg     1440

gagcuaaagg aaaacuaagu ucagggcacc uaaaaugcag auuaaaaaug gauaaauuac     1500

guuuaaaagg aguaucauau ucauugugca cagcagcauu uacauuuaca aaaauuccgg     1560

ccgaaacguu acauggcacc guuacgguag aaguacaaua cgcaggaaca gaugggccau     1620

guaaaguacc ugcccaaaug gcaguugaua ugcaaacacu gacaccgguu gggagacuaa     1680

uuacugcuaa cccaguaaua acugaaucaa cagaaaauuc aaaaaugaug uuagaauuag     1740

auccaccauu cggcgauagc uauauuguaa uaggaguagg ugaaaaaaaa auaacacacc     1800

auuggcacag gagcgguagu acaaucggca aagcauuuga agcaacuguu agaggagcua     1860

aaagaauggc aguguugggc gauacagcau gggauuuugg gaguguagga ggggcacuca     1920

acucauuagg aaaagguaua caucaaauau ucggagcagc uuuuaaauca uuauucggag     1980

gaaugagcug guuuagucaa auacuuauag gaacacuauu aaugugguua ggauuaaaua     2040

caaagaacgg aagcauauca uuaauguguu uagcacuugg aggaguuuua auauuucuaa     2100

guacagcugu cagugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7079
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 2 opt

<400>  7079
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggaau uguaggacua cuguuaacaa cagcaauggc cgccgaagua acaagaagag      120

gcagcgcaua uuauauguac uuagauagaa aagaugcugg agaagcaauc uccuuuccaa      180

caacauuagg uaugaauaaa uguuauauac aaauaaugga ucuaggacau augugugacg      240

caacaauguc uuaugaaugu ccaauguuag augaaggagu agaacccgau gauguagacu      300

gcugguguaa uacgacauca acuugggugg uauacggaac augccaccau aaaaaaggag      360

aagcaaggag aucacguaga gcaguuacac uuccuucuca uucaacuaga aaauuacaaa      420

cacgaagcca aacuugguua gaaagcagag aauauacaaa acauuuaaua agaguagaaa      480

acuggauauu cagaaaucca ggauuugcac ucgcagcagc agcaauagca uggcuauugg      540

ggucaagcac aucacaaaag guaauauacu uaguuaugau auuauuaauu gcaccagccu      600

auucuauaag auguauagga guaucuaaca gagauuuugu ugaagguaug ucaggcggaa      660

cauggguaga ugugguucuu gaacauggug gauguguaac aguaauggca caagauaaac      720

caacaguaga uauagaauua guaacaacaa caguaaguaa cauggcagag guaagaaguu      780

auuguuauga agcaaguauc ucagauaugg caagcgacuc uagaugccca acacaaggag      840

aagcauaucu agauaaacaa agugacacac aauauguaug uaaaagaaca cuaguagaua      900

gaggaugggg aaauggaugu ggacuuuuug gaaaagguuc guuaguuacu ugcgcaaaau      960

uugcauguuc aaaaaaaaug acuggaaaaa guauacaacc agaaaacuua gaguauagaa     1020

uaauguuauc aguacacggg ucucaacaua guggaaugau uguuaaugac acuggacaug     1080

agacagauga aaauagagca aaaguagaaa uaacuccaaa cucaccccgu gcagaagcga     1140

cauuaggagg auuugguuca uuaggauuag auugugaacc caggacggga cuagauuuua     1200

gugaucuaua uuaucuaacu augaauaaua aacauugguu aguacauaaa gaaugguuuc     1260

acgacauccc auuaccaugg cacgcaggag cagauacagg aacaccucau uggaauaaua     1320

aagaagcauu aguugaauuu aaagacgcuc augcaaaacg ucaaaccgua guuguacuag     1380

guagccaaga aggagcagua cauacugcau uagcaggagc auuagaagca gaaauggaug     1440

gcgcgaaagg uagauuaagc aguggccauu uaaaauguag acucaaaaug gauaaacuca     1500

gauuaaaagg aguuucauau agucuaugua cagcagcauu uacauuuaca aaaauaccag     1560

ccgaaacacu ccauggaacc guaacaguag aagugcaaua cgcaggaaca gauggaccgu     1620

gcaaaguacc agcccaaaug gcaguagaua ugcaaacauu aacaccugua ggaagauuaa     1680

uaacagcaaa uccaguaauu acagagucaa cagaaaacag caaaaugaug uuagaacuag     1740

aucccccauu cggagauagu uauauuguaa uagguguagg ugaaaaaaag aucacacauc     1800

auuggcacag aucaggauca acaauuggga aagcuuucga agcaacaguc agaggugcaa     1860

aacgaauggc aguacuagga gauacagcgu gggacuucgg aaguguaggu ggagcacuaa     1920

acucacuggg gaaaggaaua caccaaauau ucggagcagc cuuuaaauca cuauuugggg     1980

gcaugucuug guucucucaa auacuaauug gaaccuuauu aauguggcua ggacuaaaca     2040

cuaaaaaugg aagcaucagu uuaauguguc uagcacuugg agguguuuua auauucuuau     2100

cgacagccgu uuccgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7080
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 2 opt

<400>  7080
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguaggaau uguaggacua uuauuaacaa cagcuauggc ugcagaagua acaagaagag      120

gcagugcaua cuauauguau uuagauagaa augacgcugg agaagcaauu agcuucccaa      180

caacauuggg aaugaauaaa ugcuacauac aaauaaugga uuuaggccau augugugaug      240

caacaaugag uuaugaaugc cccaugcuag acgaaggagu ugaaccagau gauguagauu      300

gcugguguaa uacaacuuca acauggguug uuuaugguac augucaccau aaaaaaggag      360

aagcaaggcg uucaagacga gccguaacac ucccuucgca cucaacucga aaauuacaaa      420

cccgaagcca aacauggcua gaaucaagag aauauacaaa acaucugaua agagucgaaa      480

acuggauauu uagaaaucca ggauuugcac uagcagcagc agcaaucgca uggcugcuug      540

ggagcucaac cucucaaaaa gugaucuauc uuguaaugau acuguugaua gccccagcau      600

acucaauaag auguauaggu guaagcaaua gagauuucgu agaaggcaug ucaggaggaa      660

cauggguuga uguaguauua gaacacggag guugcguuac agugauggca caagacaaac      720

caacaguaga cauagaacua guaacaacca cuguaaguaa uauggcagaa guacgaucuu      780

auuguuauga agcuaguauu ucagauaugg caucggauuc aagguguccu acucaaggug      840

aggcauaccu agacaagcaa ucagacacuc aauacguaug caaaagaaca cuaguugaua      900

gaggaugggg aaauggaugu ggauuguucg gaaaagguuc auugguaaca ugugcaaaau      960

uugcgugcuc uaaaaaaaug acagguaaau cuauacaacc ggaaaacuua gaguauagaa     1020

uaaugcuaag uguacauggc ucucaacauu cuggaaugau uguaaaugau acaggacaug     1080

aaacagauga aaauagagca aaaguagaaa uaacgccaaa uaguccacga gcggaagcaa     1140

cacuaggagg auuugguagu uuaggauuag acugugaacc gagaacagga uuagauuuca     1200

gcgaccuaua uuauuuaacg augaauaaua aacauugguu aguacauaaa gaaugguuuc     1260

augacauucc ccucccaugg cacgcagggg cagacacagg gacaccacau uggaacaaua     1320

aagaagcacu aguagaauuu aaagaagcac acgcaaaaag acaaacaguu guaguacuag     1380

gaagucaaga aggagcagua cauacagcac uagcaggagc uuuagaagca gaaauggaug     1440

gagcaaaggg aagacuaucu ucuggacacu uaaaauguag auuaaaaaug gauaaacuga     1500

gacuaaaagg uguaucauau ucguuaugua cagcagccuu cacauuuacu aaaauaccag     1560

cagaaacucu acauggaacg guuacaguag aaguacaaua ugccggaacc gauggcccgu     1620

gcaaaguacc agcacaaaug gcaguggaua ugcaaacauu aaccccagua ggaagauuaa     1680

uaacagcaaa uccaguaaua acagaaucaa cagaaaauag caaaaugaug uuagaauuag     1740

auccaccuuu uggagauagc uacauaguaa uaggagucgg agaaaaaaaa auaacucacc     1800

auuggcacag aagcggauca acaauaggaa aagcauuuga ggccacaguu agaggagcaa     1860

aaagaauggc aguacuagga gacacugccu gggauuuugg aagcguugga ggggcauuaa     1920

auagucuugg uaaagggauu caucaaauau ucggggcagc auuuaaauca cuauuuggag     1980

gaaugaguug guucucacaa auauugauag gaacauugcu aaugugguua ggacuaaaua     2040

caaaaaaugg cagcauaagc cucauguguc uugcucuagg aggaguucua auauuuuuaa     2100

guacugcagu aagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7081
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 2 opt

<400>  7081
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguuggaau aguaggauua cuacuaacua cugcaauggc agcagaagua acuagaagag      120

gcucagcuua uuacauguau uuagacagga acgaugccgg cgaagcgaua aguuuuccua      180

ccacacuagg aaugaacaaa uguuacauac aaauaaugga uuugggacau augugugaug      240

cuacgaugag cuaugagugu ccaaugcuag acgaaggagu ugaaccugau gacguagauu      300

guugguguaa cacaacaagc acauggguug uauauggaac augucaucac aaaaaaggag      360

aagcuagaag aucaagaaga gcagucacau uaccaaguca uagcacaaga aaacuacaaa      420

caagaucaca aacaugguua gaaucaagag aauauacaaa acaccuuaua agaguugaaa      480

auuggauuuu uagaaaucca ggauuugcuu uagcagcugc agcaauagca ugguuacucg      540

guucuucaac aagccaaaaa guaaucuauc uaguaaugau auugcuaauu gccccagcgu      600

acucuauucg auguauuggc guaucaaaca gagauuucgu cgaaggaaug ucaggaggca      660

cauggguaga cguuguacua gaacacggag ggugcguaac aguaauggca caggacaaac      720

ccacaguuga uauagaauua guaaccacaa cgguaucaaa cauggcggaa guuagaucuu      780

auuguuauga ggcaaguaua agugauaugg caucagauag uagaugucca acccaaggug      840

aagcauacuu agauaaacaa ucagacacac aauacguaug uaaaaggaca cugguagaca      900

gaggaugggg aaauggaugu ggauuauuug guaaaggaag uuuaguaaca ugugcaaaau      960

uugcauguuc aaaaaaaaug acgggaaaaa guauacaacc agaaaauuua gaauauagaa     1020

uuauguuaag uguucaugga agccaacaua gcgguaugau agugaacgac acaggucacg     1080

aaacugauga aaacagggcg aaaguagaaa uaacaccaaa uaguccgcgc gcagaagcag     1140

cuuuaggagg auucggauca cuggguuuag auugcgaacc aagaacaggu uuagauuuca     1200

gugacuugua cuaccuaaca augaauaaua aacauugguu agugcacaaa gagugguuuc     1260

augauauacc auugccaugg caugccggag cagauacagg cacgccacau uggaauaaua     1320

aagaggcguu aguugaguuc aaagaugcac augccaaacg acaaacagua guaguauuag     1380

gaucacaaga aggggcaguu cacacagcau uagcaggagc auuagaagca gaaauggacg     1440

gagcuaaagg aagauuaagc ucaggacacc uaaaauguag auuaaaaaug gauaaguugc     1500

gauuaaaagg uguaucuuac ucacuuugua cagcagcuuu cacauuuaca aaaauacccg     1560

ccgaaacacu acacgguaca guaacaguag agguacaaua ugccggaaca gauggaccau     1620

guaaaguucc agcacagaug gcaguagaua ugcaaacauu aacaccuguc ggaagauuaa     1680

uaaccgcaaa uccagugaua accgaaucua cugaaaauuc aaaaaugaug uuagaauuag     1740

acccuccauu uggagauuca uacauaguua uaggaguagg agagaaaaaa auuacacauc     1800

auuggcauag auccgguuca acuauaggaa aagcauuuga agcuacagua agaggcgcaa     1860

aacguauggc aguauuagga gacacggcau gggauuucgg aucaguagga ggagcauuaa     1920

acucauuagg aaaaggaaua caccaaauuu ucggugcggc cuuuaaauca uuauuuggug     1980

gaaugagcug guuuucgcaa acauuaauag gcacuuuacu caugugguug ggacuuaaua     2040

caaaaaaugg aagcauauca cucauguguu uagcacuggg aggaguacuu auauuuuuau     2100

ccacugcagu uucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7082
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 2 opt

<400>  7082
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguagguau uguaggacuu uuauuaacua cagcaauggc agcagagguu acuagaagag      120

gaucugcgua uuauauguau cuagacagaa augacgcagg agaagcaaua ucuuuuccua      180

cgacauuagg aaugaauaaa ugcuauauac aaauaaugga uuuaggccac augugcgaug      240

caaccauguc auaugaaugu ccaauguuag augaaggagu ugaaccagau gauguagauu      300

gcugguguaa uacaacauca accuggguag uauauggaac cugucaccac aaaaaaggcg      360

aagcaagaag aaguagaaga gcaguaacau uaccaucaca uagcacacga aaacuucaaa      420

cacgaagcca aacauggcuu gaaaguagag aauauacuaa acaucuaaua agaguagaaa      480

acuggauauu cagaaaucca ggcuuugcau uagcagcagc ugccauugca uggcuauuag      540

gcaguucaac aucacaaaaa guaauuuauu uaguaaugau ucuauuaauu gcaccugcau      600

auaguauaag gugcauagga guuuccaaua gagauuuugu agaaggaaug ucaggaggua      660

cauggguaga uguaguucua gaacauggug guuguguaac aguaauggcu caggauaaac      720

caacaguaga uauagaacua guaacgacaa caguaagcaa cauggcagaa guuagaagcu      780

auugcuacga agcaaguaua ucagauaugg caagcgauuc acgaugucca acacaaggag      840

aagcuuaccu ugacaaacaa ucugauacuc aauauguaug caaaaggacg uuaguagauc      900

gugguugggg aaaugguugu ggcuuauucg gaaaaggaag ucuaguuaca ugcgccaaau      960

uugcauguag uaaaaagaug acagguaaau caauacaacc agaaaauuua gaauaucgaa     1020

ucauguuauc aguacauggu ucacaacauu cugguaugau uguaaaugac acaggacaug     1080

aaacagacga aaauagagca aaaguagaaa ucacaccaaa cucuccaaga gcugaagcaa     1140

cuuuaggagg guucggcucg uuaggauuag auugugaacc aagaacggga cuagacuuuu     1200

cagaucuaua uuauuugaca augaauaaua aacacuggcu aguucauaaa gaaugguuuc     1260

augauauacc acuaccaugg caugccggag cagacacugg aaccccccau uggaauaaca     1320

aagaagcacu aguagaauuu aaagaugcuc acgcgaaacg acaaacugua guaguacuug     1380

gaucacaaga aggagcagua cacacagcau uagcaggagc uuuagaagcu gagauggacg     1440

gcgcuaaagg acgacuaagu ucaggccauu uaaaaugcag auuaaaaaug gauaaacuaa     1500

gauuaaaagg aguuucauau ucacuaugua cagcagcuuu uacauuuacc aaaauaccug     1560

cagaaacauu gcauggaaca guuacaguag aaguacaaua cgcuggagcu gauggaccuu     1620

guaaaguacc cgcucaaaug gcaguagaua ugcagacauu aacaccaguc ggaagauuaa     1680

uuacagccaa uccaguaauu acagaaucaa cggaaaauuc aaaaaugaug uuagagcuag     1740

acccgccauu uggagauuca uauauuguga ucgguguagg cgaaaaaaaa auuacacacc     1800

auuggcauag aucagguucu acaauaggca aagcguuuga agcaacugug aggggcgcaa     1860

aaagaauggc aguauuagga gauacugcau gggauuucgg aaguguagga ggagcacuaa     1920

acucccuggg aaaagguauc caucaaauau uuggagcggc guucaaauca uuauucggug     1980

gaaugucaug guucucacaa auccucauag gaacuuuauu aaugugguua ggacuuaaua     2040

caaaaaaugg caguauauca uuaaugugcc uagcacuagg aggagucuua auauuuuuaa     2100

guacugcagu uucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7083
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 2 opt

<400>  7083
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cagugggaau aguagguuua cuacuaacaa cagcaauggc agcagaagua acaagaagag      120

gaucugcaua uuauauguau uuggauagaa acgaugcagg ggaagcaauc aguuuuccua      180

caacguuggg aaugaauaaa uguuauauac aaauaaugga ccuaggacau augugugaug      240

cuacaauguc guaugaaugc ccgaugcugg augaaggggu agaaccagau gauguagacu      300

guugguguaa uacaaccagu acaugggugg uauauggaac augucaucau aaaaaaggug      360

aggcucgacg aaguaggaga gccguaacau uaccuucaca uucaacaaga aaauuacaaa      420

caaggucaca aacaugguua gaaaguagag aauauacaaa acaucuaauu agaguugaaa      480

acuggauauu uagaaaccca ggguucgcuu uggcagcagc agcaauagca ugguuauuag      540

gaagcucaac aucucaaaaa guuauauauc uagucaugau auuacuuaua gcauuagcuu      600

auuccauacg auguaucgga guaucaaaua gagauuucgu agaaggaaug ucaggaggaa      660

cauggguuga cguaguguua gaacauggug gcuguguaac aguaauggca caagauaagc      720

caacaguaga cauugaauua guaacuacaa caguaaguaa uauggcagaa guaagaucuu      780

auuguuacga agcaaguauc agcgauaugg ccuccgauuc aagaugcccg acacaaggug      840

aagcauauuu agauaaacaa agcgauacgc aauacguaug uaaacguacc uugguagaca      900

gagguugggg uaauggaugc ggauuauuug gaaaaggcuc acuuguuacg ugugcaaaau      960

ucgcaugcuc aaaaaaaaug acuggaaaau ccauacaacc agaaaaucua gaauauagaa     1020

ucaugcuauc cguacaugga ucccaacauu cagguaugau cguaaaugau acagggcaug     1080

aaacagauga aaauagagcg aaaguagaaa uaaccccaaa uaguccaaga gcugaggcaa     1140

cuuuaggagg auuuggauca cuaggcuuag acugcgaacc gaggacaggu uuagauuuua     1200

gugaucuaua uuaucuuacu augaauaaua aacauugguu gguacauaaa gaaugguuuc     1260

acgauauacc auuaccaugg caugcaggag cugauacagg aacaccacau uggaauaaua     1320

aagaagccuu gguugaauuu aaagacgcac augcaaaacg acaaacagua gugguauuag     1380

gaagucaaga aggggccgug cacacagcau uagcuggagc ucuagaagca gaaauggaug     1440

gagcaaaagg aagacuauca ucaggacacc uaaaaugccg guuaaaaaug gacaaacuaa     1500

gacuaaaagg ggugaguuac ucauugugua cugcagcauu uacauucaca aagauaccag     1560

cagaaaccuu acaugguacu gucacaguag aaguacaaua ugcaggaaca gauggaccau     1620

guaaaguacc ugcacaaaug gcaguagaca ugcagacauu aacaccagua gguagacuaa     1680

uaacugcaaa ucccguuaua accgaaucaa ccgaaaauag uaaaaugaug uuagaauuag     1740

auccaccuuu uggggauuca uauauaguca uaggaguagg agaaaaaaaa aucacucauc     1800

auuggcacag aagcggauca acaaucggga aagcauucga agccacaguu agaggagcaa     1860

aaagaauggc agucuuagga gauacggccu gggauuuugg aaguguaggg ggggcauuaa     1920

auucauuggg aaaaggaaua caucaaauau uuggagcagc auuuaaaagu uuguucggug     1980

gaaugucaug guuuucucaa auacuaauag gaacucuauu aauguggcua ggucuuaaua     2040

caaaaaaugg gaguauauca uuaaugugcc uagcauuagg uggagucuug auauuccuaa     2100

gcacagcagu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7084
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 2 opt

<400>  7084
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguaggaau aguuggauua cucuuaacaa cagcaauggc agcagaagua acaagaaggg      120

guucagcaua uuauauguau cuagaucgaa augaugcagg agaagcaaua aguuuuccca      180

caacuuuggg aaugaacaaa uguuauauac aaauaaugga cuugggacau augugugaug      240

cuacuauguc cuaugaaugu ccaauguuag acgaaggagu agaacccgac gauguugauu      300

gcuggugcaa cacaacaagu acuuggguug uauacggaac augccaucac aagaaaggag      360

aagcaagaag aucaagacga gcaguaacuu uaccaaguca uaguacaaga aaauuacaaa      420

cacgaucaca gacauggcuc gaaucaaggg aauauacaaa acaucuaauu agaguagaaa      480

auuggauauu cagaaaucca ggauuugcau uagcagcugc agcaaucgca uggcuuuuag      540

guucaucgac uucacaaaaa guuauauauu uaguaaugau acuucuaaua gcccccgcau      600

auucaauucg gugcauugga gucucgaauc gagacuuugu ggaaggaaug ucagguggaa      660

cauggguaga uguagucuua gaacauggag gaugcguuac uguaauggcu caagauaaac      720

cuacaguaga uauagagcua guaacaacua caguaaguaa uauggcagaa guaagaaguu      780

auuguuauga ggcaaguaua ucugauaugg uaucagauuc aagaugucca acacagggag      840

aagcauaccu agauaaacag ucagauacuc aauauguuug uaaaagaaca uuaguagauc      900

gaggaugggg uaauggcugu ggauuauuug gaaaaggauc acuuguaaca ugugcgaaau      960

uugcauguuc aaagaaaaug acaggcaaau caauucaacc agaaaauuua gaauacagaa     1020

uaaugcuaag uguacacggu ucacaacauu ccggcaugau aguaaacgac acuggacaug     1080

aaacugacga aaauagagca aaaguugaaa uaacaccaaa uaguccuaga gcagaagcaa     1140

cauuaggugg auuuggauca uugggauuag auugugaacc cagaacaggu cuggauuuuu     1200

cagauuuaua uuaccuuacc augaacaaua aacauuggcu agugcauaaa gaaugguucc     1260

acgacauacc acuaccuugg caugcaggag cagauacagg aacaccacau uggaauaaua     1320

aagaggcuuu aguugaauuu aaagacgcac augcuaaaag acaaacagua guaguauuag     1380

gaagucaaga aggagcagua cauacagcac uagcuggugc auuagaagca gaaauggacg     1440

gugcaaaagg uagauuauca ucaggacacu uaaaauguag auuaaaaaug gauaaacuac     1500

gccuaaaagg uguaucauau ucucuaugua cagcagcauu uacauucaca aaaauuccug     1560

cagaaacauu acaugguaca guaacaguag aaguacagua ugcaggaacg gauggacccu     1620

guaaaguacc agcacaaaug gcaguagaua ugcaaacauu aacaccaguc ggaagauuaa     1680

uuacugccaa cccaguaauu acagaaagua cagaaaacag caaaaugaug uuagaacuag     1740

acccacccuu uggagacucu uauauaguaa uagguguagg agaaaagaaa auaacacauc     1800

auuggcaucg uagugguagc acaauaggaa aagcguucga agcaacagua agaggagcaa     1860

aaagaauggc uguauuaggu gauacagcuu gggauuucgg auccguagga ggagcacuca     1920

acaguuuagg aaaagguauu caccaaaucu uuggagcagc auuuaaaagu uuauuuggag     1980

gaaugagcug guuuagucaa auauuaauug guacucuauu aaugugguua ggauuaaaua     2040

cuaaaaaugg aucaauaagu cuaaugugcc uagcauuagg cgguguacua auauuucugu     2100

cuacagcggu uucagcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7085
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 2 opt

<400>  7085
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguugggau ugugggucuu cuuuuaacaa cagcuauggc agcagaagua acaagaagag      120

gaucagcaua cuacauguau uuagaucgaa augaugcugg agaagcaaua aguuuuccaa      180

cuacuuuagg aaugaauaaa uguuauauac aaauuaugga uuuaggacau augugugaug      240

cuacuaugag cuaugaaugu ccaaugcuag augaaggagu agaacccgac gauguagauu      300

gcugguguaa cacuacaagc acguggguug uauauggaac uugccaccau aagaaaggag      360

aagcuagaag aaguagacga gcaguaacac uaccuaguca uucaacgaga aaacuacaaa      420

ccagaucaca aacauggcua gaaucaagag aauauacaaa acaccucaua agaguugaaa      480

acuggauauu uaggaaccca ggauuugcuc uagcagcagc ugcaaucgca ugguuauuag      540

gauccucaac aucacaaaaa guuauauacu uaguaaugau uuuauuaaua gcaccagcuu      600

auuccauuag auguauaggu auaagcaaca gggacuuugu agaaggaaug aguggaggaa      660

cuugggucga uguagucuua gaacacggcg gaugcguaac uguaauggca caggauaaac      720

caacuguaga uauagaauua guaaccacaa cuguaucuaa uauggcugaa guaagaucau      780

acugcuauga agcaaguaua ucugauaugg caucagacuc aagaugccca acacaaggag      840

aagccuauuu agacaaacaa agugauacac aauacguaug uaaaaggaca uuaguagaua      900

gaggaugggg aaauggcugc ggauuauuug guaaaggaag ucuaguaacu ugugccaaau      960

ucgccuguuc aaagaaaaug acaggaaaau caauucaacc cgaaaaucuc gaauaucgua     1020

uaauguuguc aguacacgga ucacaacaua guggaaugau aguuaacgac acuggccacg     1080

aaacggacga aaaucgagca aaaguagaaa uaacaccaaa uaguccuaga gcagaagcga     1140

cauuaggagg auuuggcaua uuaggauuag auugugaacc aagaacugga cucgauuuuu     1200

cagauuuaua cuauuugaca augaauaaua aacacugguu aguacacaaa gaaugguuuc     1260

augacauacc uuuaccgugg caugcaggag cagacacagg aacgccucac uggaacaaua     1320

aagaagcauu aguggaguuc aaagaugcac acgcaaaaag acaaacagua guaguauuag     1380

guagucaaga aggagcagua cauaccgcac uagcuggcgc uuuagaagca gaaauggaug     1440

gcgcaaaagg aagauuauca agcggacauu uaaaauguag gcuaaaaaug gauaaacuaa     1500

gauuaaaagg aguaagcuau agcuuaugca cagcagcauu cacauuuaca aaaauaccag     1560

cagagacauu acaugguaca guuacuguag aaguccaaua ugcagguacu gauggaccau     1620

guaaaguacc ggcacaaaug gcaguagaca ugcaaacauu aacaccuguu gguagacuaa     1680

ucacagcaaa uccuguaaua acagaaucaa cagaaaacuc gaaaaugaug uuagaauugg     1740

acccuccauu uggagacuca uauauuguca uaggaguagg agaaaagaaa auaacucauc     1800

auuggcauag aucaggauca acaauaggaa aagcauuuga agcaacagug agaggagcca     1860

aaagaauggc gguucuugga gacacagcuu gggauuuugg aucuguagga ggagcauuaa     1920

auaguuuagg aaaaggaaua caucaaauau uuggcgccgc uuuuaaaagc cuuuuuggag     1980

gaaugucaug guuuagucaa auacugauag guacgcuauu aauguggcuu gguuugaaua     2040

cgaaaaaugg aaguauaagu uuaauguguc uggcacuugg agggguauua auauucuuau     2100

caacagcagu gucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7086
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 2 opt

<400>  7086
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

guguagggau aguaggauua cuauuaacua ccgcaauggc cgccgaagua acaagaagag      120

gcagugcaua uuacauguac uuagacagga augacgccgg ugaagcuaua aguuuuccaa      180

caacuuuagg aaugaauaag ugcuauauuc aaauaaugga cuuaggacau acgugugaug      240

caacaauguc auaugaaugu ccgauguugg acgaaggagu agaaccagau gauguagauu      300

guugguguaa cacaacgagu acauggguug uauauggaac uugucaucac aagaaaggag      360

aagcaagaag aagcagacga gcaguaaccc uucccucuca caguauaaga aaacuacaaa      420

caaggagcca aacauggcua gaaaguagag aauauacaaa acaucugauu agaguugaaa      480

auuggauauu cagaaauccu ggauuugcgu uagcagcggc agcaauagca ugguuauuag      540

guaguucaac aagucaaaaa guaauuuacc uaguaaugau auuacuaauu gcaccagcau      600

acucuauaag gugcaucgga guaucaaaua gagauuucgu agaagguaug ucaggaggaa      660

cauggguaga cguaguguua gaacauggug gauguguaac uguaauggcg caagauaaac      720

caacagugga uauagaacug guaacaacua cuguaagcaa uauggcagaa guacgaucgu      780

auuguuauga ggccaguaua ucugauaugg ccagugacag uagaugccca acacaaggug      840

aagcauaccu agacaaacaa ucagacacuc aguauguuug uaaacgaaca uuaguagauc      900

gagguugggg uaauggaugu ggguuauucg gaaaaggaag uuuaguaacu ugugcaaaau      960

uugcauguuc aaagaaaaug acaggaaaau caauacaacc agaaaaucua gaauauagaa     1020

uaauguuauc uguacauggu agccaacaca gcggaaugau uguuaacgac acaggacaug     1080

aaacggauga aaauagagcg aaaguugaaa uaaccccaaa uagcccaaga gcagaagcaa     1140

cuuuaggugg auuuggauca uuagguuuag auugugaacc aagaaccgga cuagacuuua     1200

gugaccuaua uuauuuaaca augaauaaua aacauugguu aguacauaaa gaaugguuuc     1260

acgauauacc acucccaugg cacgccggag cagauacagg uacaccacac uggaauaaua     1320

aagaagcauu aguagaguuc aaagaugcgc acgcaaaaag acaaacuguu gugguucuag     1380

gaagucaaga aggcgcaguu cauacugcau uagcgggagc acuagaggcc gaaauggacg     1440

gagcaaaagg aagacuuagc ucaggucauc uaaaaugcag auuaaaaaug gauaaacucc     1500

gauuaaaagg aguaucauac aguuuaugua cagcagcauu uacauuuaca aaaauaccag     1560

cagaaacucu acauggaacu gugacaguag aaguacaaua cgcggguacu gacggaccuu     1620

guaaaguacc agcucaaaug gccguggaua ugcaaacauu aacaccaguu ggaagauuaa     1680

uuacagcuaa cccaguaaua acugaaucaa cagaaaacag caaaaugaug uuagaauuag     1740

acccgccauu uggagacagu uauauaguaa uuggaguagg cgaaaagaaa auaacucauc     1800

acuggcaccg aucaggauca acaauaggua aagcauuuga agcaacuguu cgaggagcaa     1860

aaaggauggc aguacuaggc gauacagcgu gggauuuugg aucuguagga ggugccuuaa     1920

auucuuuagg aaaagguaua caccaaauuu uuggagcagc auuuaaauca cuauuuggug     1980

gaaugagcug guucucacaa auauuaauag gaacacuauu aauguggcuu ggucuaaaug     2040

cuaaaaaugg uaguauaagu uuaauguguu uagcauuagg aggaguacua aucuuccuaa     2100

gcaccgcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7087
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 2 opt

<400>  7087
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

ccguagguau aguaggauua cuacuaacaa cagccauggc cgcagaagua acccgucgag      120

gaucugcaua cuauauguau uuagaccgaa acgacgcagg agaagcuaua uccuucccaa      180

caacucuagg aaugaauaaa uguuauauuc aaauaaugga uuuaggacau augugcgaug      240

caaccauguc auaugaaugu ccaauguuag augaaggggu agaaccagau gauguagacu      300

gcugguguaa uacaacuucc acauggguug uauacggaac gugucaucau aagaaaggag      360

aagcgcaaag aaguagacga gcaguuacac uaccaaguca uaguacaaga aaauuacaaa      420

ccagaucuca aacauggcua gaaucaaggg aauacacuaa acaucuaaua agaguagaaa      480

auuggauuuu uagaaauccu ggauuugcac uagcggcagc cgcaauagca uggcuauuag      540

gaaguucuac aucacaaaaa guaauauacu uaguaaugau acuauuaaua gcaccagcuu      600

acaguaucag augcauagga guuucaaaca gagauuucgu agaaggcaug aguggaggaa      660

cauggguuga uguaguacua gaacauggug gaugcguaac aguaauggca caagacaaac      720

caacaguaga uauugaauua guaacaacaa ccguaucaaa uauggcugaa gucagaaguu      780

acuguuauga agcuagcaua agugauaugg caagugauuc aaggugccca acacaaggug      840

aggcuuaucu agacaaacaa agugacacac aauauguaug uaaaagaacu uuaguagacc      900

gaggaugggg aaauggaugu ggauuauucg gaaaaggauc cuuaguaacu ugcgcuaaau      960

uugcguguuc aaagaaaaug acagguaaaa guauacaacc agaaaauuua gaauauagaa     1020

uaaugcuauc aguacacgga agccaacauu caggcaugau aguaaacgau accggacaug     1080

aaaccgacga aaacagagca aaaguugaaa uuacacccaa cagcccccga gcagaagcaa     1140

cccuuggagg uuuuggcagc uuaggauuag auugugaacc aagaaccgga uuagauuuua     1200

gugaccuaua uuaucuaaca augaauaaua aacauugguu aguacacaaa gaaugguuuc     1260

acgacauacc auuaccaugg caugcaggag cagacacugg aacaccacau uggaauaaua     1320

aagaagcauu aguagaauuu aaagaugcuc augcaaaacg acaaacagua guaguauuag     1380

gcagucaaga aggggccgua cauacagccu uagcaggggc acucgaagca gaaauggacg     1440

gagcuaaagg aagacuauca aguggucauu uaaaaugucg cuuaaagaug gauaaauuga     1500

gauuaaaagg aguaaguuau ucacuaugua cagcugcauu uacauucaca aaaauaccag     1560

cagaaacacu ucauggaacc guaacaguag aaguacaaua ugcagggaca gauggaccau     1620

guaaaguacc ugcacaaaug gcaguugaua ugcaaaccuu aacaccagua ggaagacuaa     1680

uaacagcaaa uccaguaaua acagaaucga cagaaaauag uaaaaugaug uuagaauuag     1740

acccacccuu uggagacuca uauauaguga uagguguagg ugaaaagaaa auaacucacc     1800

auuggcauag auccggaagu acaauaggaa aagcauuuga agcaacagua agaggagcga     1860

aacguauggc uguacuagga gauacagcau gggauuuugg aucaguagga ggagcacuaa     1920

auagccuugg aaaaggaaua caccagauau uuggagcagc auuuaaaucu uuauuuggag     1980

gaaugucaug guucagucaa auauuaauag guacauuacu aaugugguua gguuuaaaca     2040

cuaaaaaugg aucuauaucu uuaauguguu uagcauuagg gggaguuuua auauuucuau     2100

caacagcugu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7088
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 2 opt

<400>  7088
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacuu       60

ccguagguau aguagguuua cuauuaacaa cugccauggc cgcagaagua acuagaagag      120

guagcgcaua uuauauguac uuagaucgaa acgaugccgg agaagcaaua ucauucccaa      180

caacauuagg caugaauaaa uguuauauac aaauaaugga uuuaggacau augugugaug      240

caacaauguc auacgaaugu ccgaugcuag acgaaggugu agaaccagac gauguagacu      300

guugguguaa cacaacuucc acauggguug uuuauggaac uugucaucau aaaaaaggag      360

aagcaagacg uucacguaga gcuguaaccc uaccaucaca uucaacaaga aaacuucaag      420

caagaucaca aacauggcua gagucuagag aauacacaaa acauuuaaua agaguagaaa      480

acuggauauu uagaaauccg ggauucgcuu uagcugcagc agcaaucgcc uggcuauuag      540

guucaagcac aagucaaaaa guaauauacu uagucaugau auuauugaua gccccagcuu      600

auucaauaag auguauagga guuucaaaca gggauuuugu cgaaggaaug ucaggaggaa      660

cauggguaga ugucguauua gaacacggag gauguguaac aguaauggca caagauaaac      720

caacaguuga caucgaauua guuacaacga caguaagcaa uauggcugaa guaagaucuu      780

auuguuauga agcaagcauu agugacaugg caagugauuc aaggugccca acacaaggag      840

aagcuuauuu agacaaacaa uccgauacuc aauacguaug uaaaagaaca uuaguggaua      900

gaggaugggg caauggaugu ggccuauuug guaaaggaag uuuaguaacu ugugcaaaau      960

uugcauguag uaaaaaaaug acagguaaga gcauacaacc agaaaaucua gaauaucgaa     1020

uaauguuauc uguucauggc agucaacacu caggaaugau aguaaaugac acaggacaug     1080

aaacugauga gaauagagca aagguagaaa uaacaccaaa cagcccaaga gcugaagcaa     1140

cauuaggagg guuuggauca uuaggacucg auugugaacc aaggacuggc uuagacuuuu     1200

cagauuuaua uuauuuaaca augaauaaua aacauuggcu aguacacaaa gaaugguuuc     1260

augauauucc gcuaccaugg cacgcaggag cagauacggg aacuccccau uggaauaaua     1320

aagaagccuu aguagaguuc aaagacgcac acgcaaaaag acaaacaguc guaguacuug     1380

gaagucaaga aggagcagua cauacagcuu uagcaggggc ucuugaagca gagauggacg     1440

gagcaaaagg aagacuguca agcggacauc uaaaaugcag auuaaaaaug gauaaacuaa     1500

gauuaaaagg aguaucuuac agucuuugca ccgcugcuuu uaccuuuaca aaaauaccag     1560

cagaaacuuu acauggaacu guuaccguag aaguccagua cgcuggaaca gauggcccau     1620

gcaaaguacc cgcacagaug gcuguagaua ugcaaacauu aacaccagua gguaggcuga     1680

uaacagcaaa ucccguaaua acagaaucaa cagaaaauuc aaaaaugaug cuagaacuug     1740

acccaccuuu uggcgauagu uauauaguua uaggaguagg agaaaaaaaa auaacacauc     1800

auuggcacag aucugguucc acgaucggaa aagccuuuga agcaacagua aggggagcga     1860

aaagaauggc uguacuaggu gacacagccu gggauuucgg aucaguugga ggugcacuaa     1920

auucauuagg aaaaggaaua caccaaauuu uuggagcagc auuuaaauca uuauucggcg     1980

gaaugagcug guuuucacag auuuuaauag gaacacuauu aauguggcua ggacuaaaua     2040

caaaaaaugg aucgauaagu cuaauguguc uagcacuagg aggaguauug auauuucuau     2100

caacggcagu uucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7089
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 2 opt

<400>  7089
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggcau aguaggacua uuacuaacaa cagccauggc agcggaagua acaaggagag      120

gaucugcaua uuacauguac uuagaucgau ccgaugcagg agaagcaaua aguuuuccaa      180

caacuuuagg uaugaauaaa uguuauauac aaauaaugga uuuaggacac augugugaug      240

cuacuauguc uuaugaaugc ccaauguuag acgaaggagu ggaaccugau gauguugauu      300

guugguguaa uacaacaagu acauggguag uauaugguac augucaucau aagaaaggag      360

aagcgaggag aucaaggaga gcuguaacau uaccaaguca uagcacaaga aaauuacaaa      420

caagaagcca aacuugguua gaaaguagag aauacacaaa gcauuuaaua agaguagaaa      480

auuggauauu uagaaaccca ggauuugcac uagcagcagc agcaauagca uggcuauuag      540

gcaguaguac cucacaaaaa gucauauauu uaguuaugau acuuuuaaua gccccugcau      600

auagcauuag auguauaggc guuagcaauc gagauuuugu agaagguaug ucaggaggua      660

cuuggguaga uguaguauua gaacacgggg gaugugucac cguaauggcc caagacaaac      720

caacuguaga uauagaguua guuacuacaa caguaagcaa uauggcagaa gucagaaguu      780

auugcuacga agcaagcaua agcgauaugg cuagugauag ccgauguccu acccaaggag      840

aggcguauuu agauaaacaa ucugacacgc aauauguaug uaaaagaacu cuaguagaca      900

gaggaugggg caacggaugc ggauuauuug gaaaagguag cuuaguaaca ugugcaaaau      960

uugcauguuc aaagaaaaug acuggaaaaa guauucaacc agaaaacuua gaauaucgaa     1020

uaaugcuauc aguacaugga agucaacauu caggaaugau aguaaacgac acuggacacg     1080

agacagauga aaaucgagcg aaaguagaaa uaacaccaaa uucaccuaga gcugaagcaa     1140

cauuaggugg auuugguagc uuaggauuag auugugaacc aagaaccggu cuagauuuca     1200

gcgaccuaua uuauuuaaca augaauaaua aacacuggcu aguacauaaa gaaugguuuc     1260

augauauucc ucuaccuugg cacgcuggag ccgacacugg aacaccucau uggaacaaua     1320

aagaagcguu agucgaguuc aaagaugccc acgcaaaaag acaaacugua guaguauuag     1380

gaucacaaga aggagcagua cauacagcac uagcaggagc acuagaagca gaaauggaug     1440

gagcaaaggg aagauuaagc aguggacacc uaaaauguag acuaaaaaug gacaaacuaa     1500

gauuaaaagg aguuucuuau agucuaugca cggcagcuuu cacauuuagu aaaauuccag     1560

cugaaacauu acauggcaca guaacagaag aaguccaaua ugcagguacu gauggaccau     1620

guaaaguacc agcacaaaug gcaguagaua ugcagacacu aacacccgua gguaggcuua     1680

uaacagcgaa uccaguaaua acugaaagua cugaaaauag uaaaaugaug uuagaauuag     1740

aucccccauu uggugauuca uacauaguaa uaggaguagg agaaaagaaa auuacacauc     1800

auuggcaccg uucaggauca acaaucggaa aagccuuuga agcaacuguu agaggagcaa     1860

aacgaauggc aguuuuagga gauacugcau gggauuucgg aaguguagga ggggcauuga     1920

auagcuuggg aaaagguauu caucaaauau ucggagcagc auuuaaaagu cuauuuggug     1980

guaugaguug guuuagucaa auacuaauug gaacauuacu aauguggcua gguuuaaaua     2040

cgaaaaacgg aucaaucagu uuaaugugcc uagcucuagg cggcguccua auauuuuuga     2100

guacagcagu uuccgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7090
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 2 opt

<400>  7090
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cuguaggaau aguaggacua cuacuaacua cagccauggc ugcagaggua acaagaagag      120

gaucagcaua uuauauguau uuggauaggu cagaugccgg ugaagcaaua aguuuuccua      180

cuacauuagg aaugaacaaa uguuauauac aaauaaugga uuuaggacau augugugaug      240

caacaaugag uuaugaaugc ccgaugcuag augaaggagu cgaaccagau gaugucgauu      300

guuggugcaa cacaacauca acguggguug ucuauggaac augucaccac aagaaaggug      360

aagcuagaag aucaaggaga gcaguuacau ugcccaguca uaguacaaga aaauuacaaa      420

caagaaguca aacaugguua gaaaguagag aauauacuaa acaucuaaua cguguggaaa      480

auuggauauu uagaaaucca ggauuugcgc uagcagcagc agcaauagca ugguuacuag      540

guucuaguac aagucaaaaa guaauauauc uagugaugau auuauuaauu gcacccgcau      600

auaguauaag gugcauaggu gucagcaaua gagauuuugu ggaaggaaug ucuggaggaa      660

ccuggguuga uguaguuuua gaacaugggg gaugcguuac aguaauggca caggauaaac      720

ccacaguuga uaucgaauug guuacgacaa caguuaguaa cauggcagaa guaagaucau      780

acuguuauga agcaaguaua ucagauaugg caucagauuc aagaugucca acccaaggag      840

aagcauauuu agacaaacaa agugauaccc aauacguaug uaaacgaacu cuuguagaua      900

gaggaugggg aaacggaugu ggauuauuug gaaaagguag ucuaguaacu ugcgcaaaau      960

uugcuuguuc aaagaaaaug accggaaaau caauacaacc agaaaacuua gaauauagaa     1020

uuaugcuaag uguacaugga ucucagcaca gcgggaugau aguuaacgac acaggucaug     1080

aaacagacga aaauagagca aaaguagaaa uaacaccaaa caguccaaga gcagaagcaa     1140

cccuuggugg auuugggagu uuaggucugg auugcgaacc gagaacagga uuagauuuua     1200

gugauuuaua uuaucuaaca augaauaaua aacauugguu aguacauaaa gaaugguucc     1260

augauauacc acuuccaugg cauacgggag cagacaccgg aaccccacau uggaacaaua     1320

aagaggcauu aguugaauuu aaagacgcac acgcaaaaag acaaacagua guaguacugg     1380

gcucccaaga aggagcagua cauacagcac uagcgggugc acucgaagca gaaauggaug     1440

gagcgaaagg aagauuaagc aguggccauc uaaaauguag guugaaaaug gacaaacuaa     1500

gguuaaaagg aguaaguuac agccucugua cagcugcuuu caccuuuaca aaaauaccgg     1560

cagaaacauu acacggaaca guaacaguag agguucaaua ugcaggaaca gauggaccau     1620

guaagguucc agcacaaaug gcagucgaua ugcaaacauu aacuccaguu ggaagauuaa     1680

uaacagcaaa uccuguuaua acagaaggaa cagaaaacuc aaaaaugaug uuagaacuag     1740

auccaccauu uggcgauagu uauaucguaa uaggaguagg ggaaaagaaa auaacucauc     1800

acuggcauag aagcggauca accauaggua aagcauuuga agcaacggua aggggugcaa     1860

aaagaauggc aguauuggga gacacagcuu gggacuuugg aaguguagga gguguauuaa     1920

acagucuagg aaaaggaaua caucaaauau uuggagcagc auuuaaaagu uuauuuggag     1980

gaaugagcug guuuucacaa auauuaauag gaacauuacu uaugugguug ggacuaaaua     2040

cuaaaaaugg aucgauaagc cugauguguu uagcacuagg uggaguuuua aucuuccuaa     2100

guacagcagu aagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7091
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 2 opt

<400>  7091
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau aguaggauua cuacuaacca cagcaauggc agcagaggua acaaggagag      120

gaucagcaua uuauauguau uuagaucgau cagacgcagg agaagcaaua aguuuuccaa      180

ccacgcuagg caugaauaaa uguuacauuc aaauaaugga cuuaggucau augugugaug      240

caacaauguc cuaugaaugu ccuauguuag augaaggagu ugaaccugau gauguugauu      300

guugguguaa cacaacauca acuuggguag uauaugguac augucaccau aaaaaaggag      360

aagcuagacg aaguagaaga gcuguaacac uaccaagcca cucgacuaga aaacuacaaa      420

caagaucaca aacaugguua gaaaguagag aauacacaaa acaucuuaua cgaguagaga      480

auuggauauu ucgcaaucca ggauuugcac uagcagcagc agcaauagcg ugguuauuag      540

guucaucuac aagccaaaaa guaauauauu uaguaaugau auuauuaaua gcaccagcgu      600

auucaauaag auguauagga gucaguaaua gggauuucgu agaaggaaug ucuggcggaa      660

cauggguaga uguaguacua gaacauggag gaugcguaac aguaauggcu caagauaaac      720

caacuguuga uauagaauua guaacaacaa caguaagcaa uauggcagaa guaagaucau      780

acuguuauga ggcaaguaua agugauaugg caucagauag cagauguccc acacaaggcg      840

aagcauaucu cgacaaacaa agcgacacgc aauauguaug uaaacgaacu cuaguagaua      900

gagguugggg uaacggcugu ggacuauuug gaaaaggaag uuuaguaacc ugcgcuaaau      960

ucgcauguuc aaaaaaaaug acgggaaaau caauacaacc agaaaacuua gaauauagaa     1020

uaauguuaag cguacauggc agucaacacu caggaaugau aguaaaugau acaggucaug     1080

agacugacga aaaccgagcc aaaguagaaa uaacuccaaa uucaccaaga gcugaagcaa     1140

cacuuggagg uuuuggcagu uuagguuuag acugugaacc aagaacagga cuagacuuuu     1200

ccgaucuaua uuaccuaaca augaacaaua aacauugguu aguacauaaa gaaugguuuc     1260

augacauacc auuaccuugg caugcaggag cagauaccgg aacaccucac uggaauaaua     1320

aagaagcacu aguugaauuc aaagaugcac augcuaaaag acaaacuguc guuguacuag     1380

guagucaaga aggagcaguu cauaccgcac uagcgggagc acuugaagca gaaauggacg     1440

gggcaaaagg aagauuaagc ucaggacauu uaaaaugcag acuaaaaaug gauaaacuaa     1500

gacuaaaagg agucucauau aguuuaugua cagcagcguu uacauuuaca aagauuccag     1560

cagaaacauu acauggaaca guaacuguag aaguacagua ugcaggaacu gacggaccau     1620

guaaaguucc agcacaaaug gcuguugaua ugcaaacuuu aacuccagua ggaagauuga     1680

uuacagcaaa ucccgugaua acagaaucca cagaaaauuc uaaaaugaug uuagaacucg     1740

aucccccguu uggagauagc uauauaguaa uaggaguagg agaaaaaaaa auaacacacc     1800

auuggcacag gucagguucc acaauaggua aagcuuuuga agcuacagua agaggagcua     1860

aaagaauggc uguacuagga gauacagcau gggauuuugg aagcguaggc ggagcacuua     1920

auucauuagg aaaagguauc caccaaauau ucggagccgc uuucaaaucu uuauuuggag     1980

gaaugaguug guuuagccaa auauuaauag gcacucuacu aaugugguua ggacuaaaca     2040

cuaaaaaugg aucuauauca uuaauguguu uagcauuagg cggaguucua auuuuucucu     2100

caacagcagu aucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7092
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 2 opt

<400>  7092
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau aguuggauua uuauuaacaa cagcaauggc aguagaagua acuagaagag      120

gaaaugcaua uuacauguac uuagaucgau cagaugccgg ugaagcgauu agcuuuccaa      180

caaccauggg aaugaacaaa ugcuacauac aaauaaugga ccuaggccau augugcgacg      240

cuacaauguc auacgaaugu ccuauguuag augagggagu agaaccugau gauguugacu      300

guugguguaa uacaacaagu acuuggguag uauauggaac augccaucau aagaaaggag      360

aagcaaggag aucaagacga gcaguaacac uuccaagcca uucaacuaga aaacuucaaa      420

caagaucuca aacguggcua gaaucaagag aauauacaaa acacuuaaua agaguagaaa      480

auuggauuuu ccgaaaucca ggcuuugcau uagcagcagc ugcaauagcu ugguugcuag      540

gaaguucaac aucacaaaaa guaauuuacc ucguaaugau auuauuaaua gcacccgcau      600

acucaauucg augcauagga guaaguaaca gagacuuugu cgaagguaug ucaggaggga      660

cuuggguaga uguaguacua gaacauggag guugcguaac aguaauggcu caagauaaac      720

caacaguaga cauagaauua gucacuacaa cgguaucaaa cauggccgaa guacgaucau      780

auuguuauga agcaucaaua ucagacaugg ccagugacuc cagaugucca acacaaggug      840

aagccuaucu agauaaacaa agugauacgc aauauguaug uaaacgaaca cuuguagaca      900

gagguugggg aaacggcugc gguuuauuug guaaaggaag uuuaguaaca ugugccaaau      960

uugcauguuc caagaaaaug acagguaaaa gcauacaacc cgaaaauuug gaauaucgaa     1020

uuauguuauc aguacacgga ucacaacacu cagguaugau agucaaugau acuggucacg     1080

aaacugauga aaauagagca aaagucgaaa uuacacccaa uucgccuaga gcugaagcaa     1140

cguuaggagg auuuggcuca uuaggauuag auugcgaacc aagaacaggu cucgauuucu     1200

cagacuuaua uuacuuaacc augaauaaua aacauuggcu cguucacaaa gaaugguuuc     1260

augauauacc ucuacccugg caugcaggag cagauaccgg uacaccacau uggaauaaca     1320

aagaagcgcu aguagaauuu aaagacgcac augcaaaacg acaaacugua guaguuuuag     1380

gaucacaaga aggagcagua cauacugcau uagcuggagc auuagaagca gaaauggaug     1440

gagcaaaagg aagguuaagu aguggucauu uaaaauguag auuaaaaaug gauaaguuaa     1500

gacuaaaagg agugaguuau ucacuaugca cagccgccuu uacauuuacu aaaauuccag     1560

cagaaacauu gcauggaaca gucacagugg aaguacaaua cgccggaaca gacggaccau     1620

gcaaaguccc agcacaaaug gcaguagaua ugcaaacauu aaccccagua ggaagacuaa     1680

uaacagcaaa uccuguaaua acugaaucaa cggaaaauuc aaaaaugaug uuggaauuag     1740

auccaccauu uggcgauuca uauauaguua uaggcgucgg agaaaagaaa auaacccauc     1800

auuggcacag aucagguuca acaauaggaa aagcauuuga ggccacggua agaggcgcaa     1860

aaagaauggc aguauuagga gacacagcau gggauuucgg aagcguugga ggagcacuca     1920

auagcuuagg uaaaggaauc caucaaauuu uuggagcagc guucaaauca uuauuuggug     1980

gaaugucaug guuuucacaa auauuaauag gaacuuuauu aguaugguua ggacuaaaua     2040

cuaaaaacgg aucuauaagc cuaauguguu uagcacucgg aggaguauua auuuuccuaa     2100

guacagcagu uagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7093
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 2 opt

<400>  7093
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau aguuggucua uuauugacca cagcaauggc ugccgaagug acaagaagag      120

guucagcaua uuauauguau cuugaucggu cugaugcagg agaagcgaua aguuuuccaa      180

caacucuagg aaugaacaaa uguuauauac aaauaaugga ccuagggcau augugugacg      240

caacaaugag uuaugaaugu ccaaugcuag augaaggagu agaaccagac gauguugacu      300

guugguguaa uaccaccagu acauggguag uauauggcac augucaucau aaaaaaggag      360

aagcgcgacg uaguagacgc gccguaacac ucccaucaca uucaacaaga aaacuucaaa      420

caagaagcca aaccugguua gaaaguagag aauacacaaa acaccuuaua agaguagaaa      480

acuggauauu uagaaaucca ggcuucgcau uagcagcugc agcaaucgca ugguuacucg      540

gaucaucaac aucccaaaaa guaauauauc uagugaugau acuauuaauc gcaccagcau      600

acucaauaag auguauagga guaaguaaca gagauuuugu ugaagguaug agugggggga      660

cauggguaga uguaguacua gaacauggcg gaugcguaac agucauggcu caagauaaac      720

ccacaguaga uauagaacuc guaacaacaa caguaucaaa cauggcagaa gucagaucau      780

auuguuauga ggcaaguaua agugacaugg caucagacag uaggugucca acccaaggag      840

aagcuuaucu agacaaacaa agugauaccc aauauguaug uaaaagaacu uuaguagaca      900

gaggaugggg aaauggaugc ggauuauuug gaaaagguuc guuaguaacg ugcgcaaaau      960

ucgcauguag caaaaaaaug acugguaaau caauacaacc agaaaaucua gaauacagaa     1020

uaaugcucuc ugugcacggu agucaacauu cuggaaugau aguaaaugau acaggacaug     1080

aaacggauga gaacagagca aaaguagaaa uaacaccaaa caguccaaga gcagaagcaa     1140

cacuaggggg uuucgguucu uuaggauuag auugugaacc aagaacagga cuagauuuua     1200

gcgaucuaua uuauuuaaca augaacaaca aacauugguu aguacauaaa gaaugguuuc     1260

augauauacc acucccaugg caugcaggcg ccgauacagg cacaccacau uggaauaaca     1320

aagaagcacu aguggaauuu aaagaugcac acgccaaaag acaaacugua guaguacuag     1380

gaucacaaga aggagcugua cauacagcau uagcaggugc acuagaggca gaaauggaug     1440

gagcaaaagg aagauuaucu agcggccauc uaaaaugcag gcuaaaaaug gauaaauuaa     1500

gacuaaaagg agugucauac ucucucugua cugcagcauu cacuuuuaca aaaauacccg     1560

cagaaacguu acacggaacu guaacagucg aaguucaaua ugcaggaaca gauggaccau     1620

guaaaguacc agcacaaaug gcaguagaua ugcaaacuuu aacuccaguu ggccgacuaa     1680

uuacagcaaa uccaguuauu acagagucaa cagaaaauag caaaaugaug cuagaauuag     1740

acccaccauu cggagauuca uauauaguca uaggggucgg agaaaaaaaa auuacgcauc     1800

acuggcacag aagcggauca acaauaggaa aagcuuucga agcgacagua agaggagcaa     1860

aaagaauggc aguuuuaggg gauacagcau gggacuuugg auccgucggu ggagcacuaa     1920

auagucuagg aaaaggaaua caucaaauau ucggagcggc cuuuaaaucg cuauuuggag     1980

gcaugucaug guuuagucaa auucuuauag guacuuuacu aaugugguua ggguuaaaua     2040

cgaaaaacgg gucaauaagu uuaguauguu uagcucuagg aggagugcuc auauuccuau     2100

caacagcggu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7094
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 2 opt

<400>  7094
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguaggaau uguaggauua uuacugacaa cugcuauggc agcagagguu acaagaagag      120

gaagcgcaua uuauauguau cuagauagau cagaugcugg ugaagcaauu ucuuuuccaa      180

caacauuagg aaugaauaaa uguuacauac aaauuaugga uuuaggacau augugugacg      240

cuaccaugag uuaugaaugu ccaauguuag augaaggagu ugaaccagau gacguagauu      300

guugguguaa uacaacauca acauggguag uauaugggac augccaucau aagaaaggag      360

aagcaagacg aaguagacga gcuguaacau ugccaucaca uucaacaaga aaauugcaaa      420

cuagaucaca aacuuggcua gaaaguagag aauauacaaa acauuuaauc cgaguagaga      480

auuggauauu ccgaaauccu ggauuugcau uagcagcagc agcaauagcg uggcuucucg      540

guaguucaac aucacaaaaa guuauauacu uggucaugau auuauuaaua gcuccggcau      600

auagcaucag auguaucgga guaaauaauc gagauuucgu agaaggaaug ucagguggaa      660

cguggguaga uguaguauua gaacacggug gauguguaac uguaauggca caagauaaac      720

caacuguuga uauagaacuc guaacaacga caguaaguaa uauggcggaa guacgaaguu      780

auuguuauga agcauccaua ucugauaugg caucagacag cagaugucca acccaaggag      840

aagcauaccu ggauaaacaa ucagauacgc aauauguaug uaaaagaacu uuaguugaca      900

gaggaugggg aaauggaugc ggcuuauucg gcaaaggaag uuuaguaaca ugcgcaaaau      960

uugcguguag uaagaaaaug acaggaaaaa gcauacagcc agaaaaccua gaauauagga     1020

uaauguugag uguacauggc agucaacaua guggaaugau agucaaugau acuggccacg     1080

aaacagauga aaauagggca aaaguagaaa ucacaccaaa uagcccucga gcagaagcaa     1140

cauuaggagg auuuggaucu uuaggucuag auugugaacc aagaacaggc uuagauuuuu     1200

cagacuuaua cuaucuuaca augaauaaca aacauuggcu aguacauaaa gaaugguuuc     1260

acgauauacc acuaccuugg caugcaggag cagacacugg aacaccacau uggaauaaca     1320

aagaagcacu cguagaauuu aaagacgcac augcaaaaag acagacagug guaguuuuag     1380

gaucacaaga gggagcagua cauaccgcau uagcaggagc ucucgaagcu gaaauggaug     1440

gagcaaaagg aagauuauca agcggacauu uaaaaugcag acuaaaaaug gacaaauuaa     1500

gauuaaaagg uguaaguuac agucuaugua cugcagcauu uacauucacu aaaauaccag     1560

cggaaaccuu acauggaacg guaacaguag aagugcagua cgcugguacg gauggacccu     1620

guaaaguccc ugcacaaaug gccguagaua ugcaaacucu aacaccugua ggacgacuaa     1680

uuacagcuaa uccaguaaua accgaaagca cagaaaacuc uaaaaugaug uuagaauuag     1740

auccuccuuu uggagauuca uacauuguua uaggaguagg cgaaaagaaa aucacacacc     1800

auuggcauag auccggaagu acaauaggaa aagcauuuga ggccacagua agaggcgcaa     1860

aaagaauggc aguacuagga gacacagccu gggauuuugg uucuguagga ggagcauuaa     1920

acaguuuagg caaaggaaua caucagauuu ucggagcagc auuuaaauca uuauucgggg     1980

gaaugaguug guuuucacaa auacuuauag gaacacuacu aauguggcug gguuuaaaua     2040

cuaaaaaugg aagcauaucc cuaguuugcc uagcacuagg aggaguucug auauuucuau     2100

caacagcugu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7095
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 2 opt

<400>  7095
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacgu       60

caguaggaau aguaggguua cuuuuaacga cagcaauggc aguagaagua acacgaagag      120

gauccgcaua uuacauguau cuugaccgau cagaugcagg agaagcaaua aguuucccaa      180

caacacuagg aaugaauaaa ugcuauauac aaauaaugga uuuaggucac augugcgaug      240

caacaauguc cuaugaaugu ccgauguuag augaaggugu ggaaccugau gauguagauu      300

guugguguaa uacuacaucu acauggguug ucuacggcac augucaucau aagaaaggag      360

aagcgcgaag gaguaggaga gcaguaacac uaccgucaca cagcacaaga aaauuacaaa      420

cuagaucaca aaccugguug gaauccagag aauauacaaa acauuuaauc agaguagaaa      480

auuggauauu uagaaaucca ggguuugcuu uagcagcagc agcaauagca ugguuauuag      540

gaaguucaac uagccaaaaa guuaucuacc uaguaaugau ucuauuaaua gcaccagcgu      600

auucaauaag auguauaggg guaaguaaua gagacuucgu agaaggaaug aguggaggaa      660

cuuggguaga cgugguauua gaacauggag gaugcguaac agugauggca caagacaaac      720

cagcaguaga cauagaauua guaacaacaa caguaagcaa uauggcagaa guaagauccu      780

auuguuauga agcaucaaua ucagauaugg caagcgacuc gagauguccg acacaaggag      840

aggcuuaucu agacaaacaa agcgauaccc aauauguaug caaaagaaca uuaguagaua      900

gaggaugggg uaaugguugc ggauuauucg gaaaaggauc ucuagucaca ugugcuaaau      960

ucgcauguuc aaagaaaaug acaggcaaaa guauacaacc agaaaauuua gaauacagaa     1020

uaauguuaag cguucaugga agucaacaua guggaaugau aguaaacgau acgggacacg     1080

aaacggauga aaauagagcg aaaguggaaa uaacaccuaa uucaccaaga gcagaagcua     1140

cacuaggugg auuuggaucu cuaggacugg acugugaacc caggacaggu cuagauuuua     1200

gcgaccuaua uuacuuaacg augaauaaua aacauuggcu aguucauaaa gaaugguuuc     1260

augacauacc auuaccaugg caugcuggag ccgacacagg uacaccacau uggaauaaua     1320

aagaagcauu aguagaguuc aaagaugcuc augccaaaag gcagacaguu guuguacuag     1380

ggucacaaga aggagcagua cauacagcuc uagcgggagc acuagaagca gaaauggaug     1440

gagcaaaagg gcgauuaagc ucuggacauu uaaaauguag auuaaaaaug gauaaauuaa     1500

gauuaaaagg aguaaguuau ucauuaugca cagcggccuu uacauucaca aaaauaccag     1560

cagaaacacu acaugggaca gugacaguag aaguucaaua ugcaggaaca gacggaccuu     1620

gcaaaguucc ggcacaaaug gcaguugaua ugcaaacacu aacaccagug ggaagauuaa     1680

uaacagcaaa cccaguuaua acagaaagua cagaaaauag caaaaugaug cuggaauuag     1740

auccaccauu uggugacagu uacauaguua uaggaguggg agaaaagaaa auaacacauc     1800

auuggcauag aucugggagc acaauuggaa aagcguucga agccacagua aggggugcaa     1860

aaagaauggc aguauuagga gauacugcau gggauuuugg aucaguaggg ggugcuuuaa     1920

auucauuagg aaaaggaaua caucaaauuu ucggugcagc auuuaaaagu uuauuuggag     1980

gaaugucaug guucagucaa auccucauug gaaccuuauu agucuggcug ggauuaaaua     2040

cuaaaaacgg uucuauaagu uuaaccuguc uagcacuagg aggcguauua auuuucuuaa     2100

gcacagcagu uagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7096
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 2 opt

<400>  7096
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacgu       60

caguagggau agucggauua uuauuaacaa cugcuauggc aguugaaguu acaagaagag      120

gaagugcaua cuauauguau cuugauagau cagaugcggg ugaagcaaua ucauuuccua      180

cuacacuugg aaugaacaaa uguuauauac aaauaaugga ucuaggacau augugugaug      240

caacaaugag cuacgaaugu ccgauguuag acgaaggagu agagccagac gauguagauu      300

guugguguaa cacaacauca acaugggucg uuuauggaac gugucaccac aaaaaaggag      360

aagcaagaag aucgcgaaga gcaguuacac uaccaagcca uaguaccaga aaacuacaaa      420

ccagaucaca aacauggcua gaaucacgug aauauacaaa acaucuaaua cgaguugaaa      480

auuggauauu uagaaaucca gguuuugcac uagcagcagc aguaauagca uggcuacuag      540

gaucgucaac aagucaaaaa gucauauacu uagucaugau ccuacuaaua gcaccagcuu      600

acuccauaag auguauagga guaagcaaca gggauuuugu ugaagguaug aguggcggaa      660

cauggguuga uguuguacua gaacacgggg guuguguaac cguuauggca caagacaaac      720

caacaguaga cauagaacug guaacaacaa caguaucaaa uauggcagaa guucgaaguu      780

auuguuauga agcaaguauu agcgacaugg cuagugauuc aagaugucca acacaaggug      840

aagcauauuu agauaaacaa ucugauacac aauacgucug uaaaagaaca cuugucgaca      900

gaggaugggg aaauggaugu gguuuauuug gaaaaggaag ccucguaacu ugugcuaaau      960

uugcauguag uaaaaaaaug acgggaaaaa guauacaacc agaaaauuua gaauauagaa     1020

uaauguuaag ugugcacgga ucacagcauu cagguaugau uguaaacgac acaggacaug     1080

agacugacga aaauagagca aaaguagaaa uaacaccgaa cagcccuaga gcagaagcaa     1140

cacuuggagg uuuuggauca uuaggucuug auugugaacc uagaacaggu uuagauuuca     1200

gcgacuuaua uuaucuaaca augaacaaua aacauugguu agugcacaaa gaaugguuuc     1260

acgacauacc gcugccaugg caugcaggag cagacacagg aacaccucau uggaauaaua     1320

aagaagcgcu aguugaauuu aaagaugcac acgcaaaaag acaaacagua guuguacuag     1380

gaagccaaga aggcgcaguu cauacagcgu uagcaggagc ucuagaggca gagauggaug     1440

gcgcaaaagg gagauuaagu aguggacauu ugaaauguag acuaaaaaug gauaaauuaa     1500

gauuaaaagg aguaucauau agccuaugca cagcagccuu uacauuuaca aaaauaccag     1560

cagaaacauu acauggaacu guaacugugg aaguacaaua ugcaggaaca gauggcccau     1620

guaaaguacc agcacaaaug gcugucgaca ugcaaacacu aacaccaguu ggaagguuga     1680

uaacugcaaa uccagugauu acagaaucaa cagaaaauuc aaaaaugaug uuagaguuag     1740

acccaccauu uggagauagc uacauaguaa uuggcguagg agaaaaaaaa auuacgcauc     1800

auuggcacag aagcggaucc acaauaggaa aagcauuuga agcaacagua agaggagcaa     1860

aaaggauggc aguguuagga gauacagcau gggauuuugg aagugucgga ggugcucuaa     1920

acucauuagg uaaagguaua caucaaaucu uuggggcagc auuuaagucg cuauuuggag     1980

gaaugagcug guuuagccaa aucuuaauug guacacuacu agucuggcua ggauuaaaca     2040

caaaaaaugg uucaauaagc uuaaccuguc uagcauuagg gggcguacua aucuucuuau     2100

caacagcagu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7097
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 2 opt

<400>  7097
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguggguau cguaggauua uuacuuacaa cagcaauggc aguugaagua acaaggaggg      120

gauccgcaua uuauauguac uuagacagaa gugacgccgg ugaagcaaua ucauuuccaa      180

caacacuagg aguuaauaaa uguuauauac aaauuaugga ccuaggacac augugugaug      240

caacaauguc cuacgaaugu ccaaugcuag acgaaggagu ggaaccagau gauguagauu      300

guugguguaa uacaacaagu acauggguag uuuaugggac augucaucau aagaaaggag      360

aagcacgaag aucaaggaga gcugucacau uaccaucaca caguacgaga aaacuacaaa      420

caagaaguca aacauggcua gaaucaaggg aauauacuaa acaucuuaua aggguugaaa      480

auuggauauu cagaaaucca ggauucgcac uagcagcugc agcuauagcg uggcuuuugg      540

guaguaguac uagccaaaaa gucauauauu ugguuaugau ucuacuaauu gcaccggcau      600

auuccauacg uuguauagga guaucaaaua gagacuuugu ugaaggaaug ucaggaggaa      660

ccuggguaga uguaguacua gaacacggag gcuguguuac ggucauggca caagauaaac      720

caacaguaga uauagaacug guuacaacaa caguaagcaa uauggcugaa guacgaagcu      780

acugcuacga agcaaguaua ucagauaugg caucagacuc acgaugucca acacaaggag      840

aagcauauuu agauaaacaa agugauacuc aauauguaug uaaaagaacu cuaguagaua      900

gaggaugggg gaaugggugc gguuuauucg gaaaagguag ucuaguuacc ugugccaaau      960

uugcaugcuc aaagaaaaug acuggaaaaa guauacagcc ugaaaauuua gaauauagaa     1020

uaaugcuauc aguacaugga agucaacauu ccggaaugau agucaaugau acaggacaug     1080

aaacagauga aaauagagca aaaguagaaa uuacaccaaa caguccaaga gcagaagcaa     1140

cauuaggagg uuuugguuca uuaggauuag auugcgaacc cagaacagga cuagacuuua     1200

gugacuuaua uuaccuaaca augaauaaua aacauugguu agugcauaaa gaaugguuuc     1260

augauauacc auuaccaugg cacgcaggag cugauacggg aacaccacac uggaauaaua     1320

aagaggcucu gguagaauuu aaagaugcac acgcaaaaag acaaacagua guuguuuugg     1380

guagccaaga aggugcaguu cacacagcgu uggccggagc auuagaggcc gaaauggaug     1440

gugcaaaagg aagacuauca aguggacauc uaaaauguag acuaaaaaug gacaaauuaa     1500

gauuaaaagg aguaaguuau agccuaugca cagcugcauu uacauucaca aaaauaccag     1560

ccgaaacauu acauggaaca guaacaguag aaguucaaua ugcaggaaca gacggaccau     1620

gcaaaguacc ugcacaaaug gcugucgaua ugcaaacauu aacuccaguu ggaagauuga     1680

uaacagcaaa uccaguaaua acagaaagua ccgaaaacag caaaaugaug uuagaacuug     1740

auccaccuuu uggugauagu uauauaguga uuggcguagg agauaagaaa auuacacacc     1800

auugguauag aucaggauca acaauaggaa aagcauucga agcaacugua agaggagcaa     1860

aacgaauggc aguauuagga gacacugcau gggauuuugg aagcguagga ggagcguuaa     1920

auucacuagg caaaggaaua caucaaauau uuggagcagc auuuaaaagu uuguuuggcg     1980

gaaugagcug guuuagucaa auauuaauag gaacauuauu aguaugguua ggacuaaaca     2040

cgaaaaaugg aucaauaagu cuaacauguu uagcucuagg uggaguauua auauuuuuaa     2100

guacagcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7098
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 2 opt

<400>  7098
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguuggaau aguuggacua uuacucacua cagcaauggc aguagaggua acacgaagag      120

gcucagcaua uuauauguac cuugauaggu cagaugccgg aaaagcaauu ucauuuccaa      180

caacauuagg uguaaacaaa ugcuauauac aaauaaugga ucuaggccau augugugaug      240

caacuauguc auaugaaugc ccaauguuag augaaggagu agaaccagau gauguagacu      300

guugguguaa uacuacaagu acuuggguag uauauggaac augucaccau aaaaaaggag      360

aagcaagaag aagcaggaga gcagucacau ugcccucaca uucuacaaga aaauugcaaa      420

cgagaagcca aacuugguug gaaucaagag aauacacaaa acacuuaaua agaguagaaa      480

auuggauauu uagaaaccca ggauucgcau uggccgcagc agcuauagca ugguuauuag      540

gaucuucuac gucgcaaaag guaauauauc uaguaaugau auuauuaaua gcccccgcgu      600

auucaauuag auguauugga guaucaaaua gagacuucgu agaagggaug ucaggaggaa      660

caugggucga uguuguuuua gaacacggug gaugcgucac cguuauggca caagauaaac      720

cuacagucga uauugaacua guaacuacua cugucucaaa cauggcagaa guucgaaguu      780

auuguuauga agcaucaauu ucagauaugg caucagauag uagaugucca acccaaggag      840

aagcuuauuu agacaaacaa agcgacacac aauauguuug uaaaagaaca uuaguugaua      900

ggggcugggg aaaugguugu ggauuauucg gaaaaggaag uuuaguuaca ugugcaaaau      960

ucgcuuguuc uaaaaaaaug acgggaaaaa guauccaacc ugagaauuua gaauauagaa     1020

uaaugcuauc aguacacgga agucaacaca gcgggaugau aguaaaugac auaggacaug     1080

aaacggauga aaauagggcu aaaguagaaa uaacaccgaa cucaccaaga gccgaagcaa     1140

cacuuggugg uuucggauca uuaggacugg auugcgaacc aagaacugga uuagacuuca     1200

gcgaucuaua uuacuuaaca augaauaaua aacacugguu aguacauaaa gaaugguucc     1260

augauauacc auuacccugg caugcaggag cagauacggg aacaccacau uggaauaaca     1320

aagaagcccu aguagaauuc aaagaugcac augccaaaag acaaacagua guaguucuag     1380

ggagucaaga aggagcagua cauacugcac uagcaggagc auuggaagca gagauggaug     1440

gagcaaaagg aagauuauca aguggucauu uaaaaugcag auugaaaaug gauaaauuac     1500

gauuaaaagg aguaucauac ucucuuugca cagcagcauu uacuuucacu aaaauuccag     1560

cugaaacucu ucauggaaca guaacaguag aaguccagua cgcgggaaca gauggaccau     1620

gcaaaguacc ugcacaaaug gcaguagaca ugcaaacauu aaccccagua ggaagauuaa     1680

uaacagcaaa cccuguaaua acagaaagua cugaaaacuc uaaaaugaug uuagaauuag     1740

auccaccuuu uggagauuca uauauaguca uaggaguugg agauaaaaaa auaacacacc     1800

acuggcauag gucaggaagc acaauuggaa aagcauuuga agcuaccgua agaggcgcaa     1860

aaagaauggc aguauuagga gacacagcau gggauuucgg uucuguagga ggugcguuaa     1920

acucauuggg uaaagggaua caccaaaucu uuggagccgc cuuuaagagc cuauuuggag     1980

gaaugaguug guuuagucaa auacuaauag gaacacuauu aguaugguug ggacuaaaua     2040

caaaaaaugg aagcauauca cuuacauguu uagcauuagg agguguauua auauuucugu     2100

caacugcagu uagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7099
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 2 opt

<400>  7099
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guauaggaau uguaggucua cuucucacua cagcaauggc agcugagaua acaagacggg      120

guagugcaua cuauauguac cuagacagaa gugacgcagg uaaagcaauu ucauuugcaa      180

cuacauuagg cguaaacaaa ugucauguac aaauaaugga uuuaggacau augugcgacg      240

caacaauguc auaugaaugc ccuaugcugg augagggagu ugaaccugac gauguagauu      300

guugguguaa uacaacauca acauggguag ucuacggaac augccaucau aagaaaggag      360

aagcaagaag aaguaggaga gcagugacac uaccuucuca uaguacaaga aaauuacaaa      420

cuagaaguca aacauggcua gaaucaagag aauauacaaa acauuuaaua aaaguugaaa      480

auuggauauu uagaaaucca ggauuuacau uaguagcugu ugcaauagca uggcuauugg      540

gaucaaguac cagucaaaaa guuauuuauc uaguaaugau cuuauuaaua gccccugcau      600

auagcauacg auguauagga guuucaaaca gagauuuugu agaaggaaug aguggaggua      660

ccuggguaga uguaguauua gaacauggag gcugcguaac ggugauggcu caagauaagc      720

caacaguuga uauagaauua guaacaacaa caguaagcaa cauggcagaa guuagaagcu      780

acuguuacga agcaaguaua agugauaugg caucagauag uagaugccca acgcaaggag      840

aagccuacuu agauaaacaa agugauacac aauauguaug caaaagaaca uuaguugauc      900

gaggaugggg aaacgguugc gguuuauuug gaaaaggauc uuuaguaacu ugugcaaagu      960

uuacauguag uaagaaaaug acugguaaaa gcauacaacc agaaaaucua gaauauagaa     1020

uaauguuauc aguacaugga agccaacaca gcggaaugau aguuaaugac accggacacg     1080

aaacagacga aaauagagcc aaaguagaag uuacaccaaa caguccucga gcggaagcaa     1140

cacuaggcgg auucggaagu uuaggauuag acugugaacc uagaacaggc cuagauuuua     1200

gugacuuaua cuacuuaaca augaauaaca aacauugguu aguacacaaa gaaugguucc     1260

augacauccc acuaccaugg cacgcuggug cagauacugg aacaccacau uggaauaaua     1320

aagaagcauu aguagaauuu aaagaugcac augcuaaaag acaaacaguu guuguuuuag     1380

gaucucaaga aggagcagua cauacagcac uagcuggcgc ucuggaggcu gaaauggaug     1440

gagcgaaagg aagauuauuc ucaggacacu uaaaauguag auuaaaaaug gauaaauuga     1500

gacuaaaggg aguaaguuau agucuaugua cagcagcauu uacguuuaca aaagugccug     1560

cagaaacacu acauggaacu gucacgguag aaguucaaua ugcaggaacu gauggaccgu     1620

guaaaguacc agcacaaaug gcaguagaua ugcaaacauu aaccccugua ggaagacuga     1680

uaacagcaaa cccgguaaua acagaaagua cagaaaacag uaaaaugaug uuagaauugg     1740

acccgccauu cggagauagc uauauaguaa uuggcguagg agauaagaaa auaacucacc     1800

auuggcacag aaguggauca acaauaggaa aagcauucga agcaacagua agaggagcaa     1860

aacgaauggc aguauuggga gauacagcau gggauuuugg aucaguagga ggcguauuua     1920

acucgcuagg aaaagggaua caucaaaucu uuggagcagc cuucaaaagu uuauuuggag     1980

gaaugagcug guuuagucaa auacuuauag gaacacuauu aguaugguua ggauuaaaca     2040

cuaaaaacgg aaguaucucu uuaacauguu uagcauuagg aggagugaug auauucuuau     2100

cgacagcugu gagcgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7100
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 2 opt

<400>  7100
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guauaggaau aauaggucuc uuguuaacga cagcuauggc agcagaaaua acaagacgag      120

ggagugcaua uuauauguac cuagaccguu cugacgccgg aaaagcuaua ucauuugcca      180

caaaccucgg aguaaacaag ugucauguac aaauaaugga ucucggucau augugugacg      240

cgacuaugag uuaugaaugu ccaaugcuag augaaggagu agaaccagac gacguugauu      300

gcugguguaa uacaacaagc acuuggguag uauauggaac augucaucac aagaaagggg      360

aggccagaag aucaagacga gcaguaacau uaccauccca cagcacaaga aaauuacaaa      420

caagaaguca aacaugguua gaaaguagag aauauacuaa acauuuaaua aaaguugaaa      480

auuggauuuu cagaaaucca ggauucgcau uagcagcagu agcaauagcc uggcuauuag      540

gaucaucaac uucacaaaaa guaauuuauc uggucaugau auuacuaaua gcaccagcau      600

auagcauaag augcauagga guaaguaaca gagauuuugu agaaggaaug aguggaggaa      660

cauggguuga ugucguauua gaacauggag guuguguaac uguuauggcg caagauaaac      720

caacaguaga caucgaauua guuacaacaa caguaagcaa uauggcugaa guaaggaguu      780

auuguuauga agcauccauu ucggauaugg ccucagauag uagaugcccu acacaaggag      840

aagcauauuu agauaaacaa ucagacacac aauaugucug caaacgaacu cuagucgaua      900

gaggaugggg uaauggcugu ggccuauucg guaaaggauc ccuaguaaca ugcgcaaaau      960

ucacaugcuc uaagaaaaug acagggaaau caauccaacc ugaaaauuug gaauaucgaa     1020

uaauguuaag uguacacggg agucaacacu caggaaugau aguaaaugau auagggcaug     1080

aaacagauga aaacagagca aaaguagaag uaacgccuaa uucaccaaga gcugaagcaa     1140

cacuaggugg auuugggagu uuaggauuag acugugaacc aagaacagga uuagauuucu     1200

cagaccucua cuacuuaaca augaauaaua aacacuggcu aguacauaaa gaaugguucc     1260

augauauucc uuuaccaugg cacgcaggag cagauacagg cacaccucau uggaacaaua     1320

aagaagcucu aguagaauuu aaagaugcac augcaaaaag acaaacugug guuguauuag     1380

guucacaaga aggagcagua cauacagcac uagcuggcgc auuggaagca gaaauggacg     1440

gagcaaaagg uaggcucuuu aguggacauc uaaaauguag guuaaaaaug gacaaacuua     1500

gguuaaaagg aguaucauac ucacuaugca cugcugcuuu cacauucaca aaaguacccg     1560

cagaaacauu acacggaaca gugacaguug aaguacaaua ugcuggaacu gauggucccu     1620

guaagguacc agcacaaaug gccguagaua ugcaaacauu aacuccagua gguagacuaa     1680

uaaccgcaaa uccaguaauu acagaaucca cagaaaauag uaagaugaug cuagagcuag     1740

auccaccauu uggagauagu uauauaguca uaggaguagg ugacaagaaa aucacacauc     1800

acuggcauag aaguggcagc acaauuggaa aagcauuuga agcaacagua agaggagcaa     1860

aaagaauggc aguauuaggu gauacagcau gggauuuugg aaguguaggu ggaguuuuua     1920

auucacuagg aaaaggagua caucaaauau uuggagccgc uuucaaauca cuauuuggag     1980

gaaugaguug guuuucucaa auauuaauag gaacauuauu aguaugguua ggucuaaaca     2040

cuaaaaaugg aucaaucuca uuaacauguu uagcacucgg aggaguuaug auuuuccuaa     2100

gcacagcagu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7101
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 2 opt

<400>  7101
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacuu       60

caauagguau aauaggucua uuacuaacaa cagcaauggc ugcagaaaua acacgaagag      120

gaucagcuua cuacauguau cuagacagaa gugaugcugg aaaagcaauu agcuucgcaa      180

caacacucgg aguuaauaaa ugccacguuc aaauaaugga cuuaggacau augugugaug      240

caacuaugag uuacgaaugu ccaaugcuug acgaaggugu ugaaccagau gauguagacu      300

guugguguaa uacgacuagc acguggguag uauacggaac augucaucau aaaaaaggcg      360

aagcuagaag aagucgacga gcaguuacac uaccgaguca caguacaaga aaacuacaaa      420

cacgaucaca aacaugguua gaaaguagag aauauacaaa acaccuaaua aagguugaga      480

auuggaucuu cagaaauccg ggguucgcuc uuguagcagu agccauagca ugguuauuag      540

gaucuagcac aucacaaaaa guaaucuauc uaguuaugau acuuuugaua gcaccagcuu      600

auaguauaag auguauugga guaucaaaua gagacuucgu agaaggaaug ucuggaggaa      660

ccuggguaga uguaguauua gaacacggcg gaugcguuac aguaauggcc caagauaaac      720

caacaguaga cauagaauua guaaccacga caguaaguaa uauggcggaa gugagaaguu      780

auugcuauga agcaaguaua ucagauaugg caucagauuc acgaugccca acacaaggag      840

aagccuauuu agauaaacaa agcgauacac aauacguuug caaaagaacu cuaguagaca      900

gaggaugggg aaauggaugu ggauuauucg gaaaaggauc uuuaguaacc ugugcaaaau      960

uuacaugcag uaaaaaaaug acagguaaaa guauacaacc agaaaauuua gaauauagaa     1020

ucaugcuuuc aguacacgga ucacaacaua gcgguaugau aguaaacgac auaggacaug     1080

aaaccgauga aaauagggca aaaguagagg uaacaccaaa cucaccaaga gcagaagcua     1140

cuuuaggagg auuuggauca uuaggguugg auugugaacc acgaaccgga cuagauuuua     1200

gugaucuaua cuaucuaaca augaauaaca aacauugguu gguacauaaa gaaugguuuc     1260

acgacauucc ucuaccaugg caugcuggug cagauacugg aacaccacau uggaauaaua     1320

aagaagcacu aguagaguuu aaagaugccc augccaaacg acaaacagua guaguacuag     1380

gaagucaaga aggagcagua cauacagcau uagcuggagc auuagaagcg gaaauggaug     1440

gagcaaaagg aagacuauuc aguggucacc uaaaauguag auuaaaaaug gauaaauuaa     1500

gauuaaaagg agugucauau ucccuaugca cagcggcauu cacauuuacg aaaguuccag     1560

cagaaacacu ccauggaaca gugacaguag agguacaaua cgcuggaacu gaugguccau     1620

gcaaaguacc ggcacaaaug gcgguugaua ugcagacauu aacaccagua gggagguuaa     1680

uaacagcaaa uccaguaauu acagaaagca cagaaaacuc uaaaaugaug cuagaguuag     1740

auccacccuu uggagacagu uacaucguaa uaggaguagg agauaaaaaa auaacgcauc     1800

auuggcauag aucagguucu acuauuggca aagcuuuuga ggcaacagua agaggagcaa     1860

aaaggauggc agucuuaggu gauacggcau gggauuuugg cucuguagga ggaguuuuua     1920

acaguuuagg uaaaggaauu caccaaauau uuggagcggc auuuaaaagc uuauuuggag     1980

gaaugaguug guuuucacaa auacuuauag guacguuauu agucuggcuu ggauuaaaca     2040

caaaaaaugg aaguauauca cuaacuugcu uagcauuagg aggcguaaug auauuuuuaa     2100

guacugcagu aagugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7102
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 2 opt

<400>  7102
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcauuggaau uauaggacua uuauuaacaa ccgcaauggc cgcagaaaua acgagacgug      120

gaagcgcaua cuauauguau uuagaccgau cugaugcagg aaaagcuauu ucuuuugcga      180

caaauuuagg aguuaauaaa ugccauguuc aaauaaugga ucuaggacau augugugaug      240

caacaauguc auaugaaugu ccaauguuag augaaggagu agaaccagac gacguagauu      300

guuggugcaa cacaacauca acauggguag uauacggaac gugucaccau aagaaaggug      360

aagcaagaag aucuaggaga gcaguaacau uaccaucgca uagcacaaga aaacuacaaa      420

caagaaguca aaccuggcua gaaucaagag aauacacaaa acaccucaua aaaguggaaa      480

auuggauauu cagaaaccca ggauucgcuu uagcagcagu agcaauagca uggcuuuuag      540

gaagcaguac aucccaaaaa guaauauauu uaguuaugau auuauuaaua gcuccagcau      600

acucaaucag gugcauagga guaaguaaua gggauuucgu ugaaggaaug ucaggaggaa      660

cauggguaga cguaguauua gagcacggag guugcguaac gguaauggca caagacaaac      720

caacagugga uauagaauua guaacaacaa cuguuaguaa uauggcggaa guacgaucau      780

auugcuauga ggccucuaua ucagacaugg caucagacag cagaugccca acccaaggag      840

aggcuuacuu agauaaacaa agugacacac aauacguaug uaaaagaaca cucguugauc      900

gaggcugggg aaauggaugc ggauuauuug gaaaaggauc uuuaguuaca ugcgcaaaau      960

uuacauguag uaagaaaaug acagggaaaa gcauacaacc agaaaauuua gaauauagaa     1020

uaaugcuauc uguacaugga ucacaacauu cagggaugau aguaaacgau gaaaaccgag     1080

caaaagucga aguaacucca aauucaccuc gagcggaagc aacguuaggu gguuucggua     1140

guuuagguuu agauugcgaa ccuagaacag gacuggauuu cagcgauuua uauuaccuaa     1200

cuaugaacaa uaaacauugg uuagugcaua aagaaugguu ucacgauaua ccacucccau     1260

ggcacgcagg agcugauaca ggaacaccac auuggaauaa uaaagaagcu cuaguagaau     1320

uuaaagaugc acacgcaaaa agacaaacag uagugguacu aggaagucaa gaaggagccg     1380

uacauacagc cuuagcagga gcauuagaag cugaaaugga uggugcaaaa ggaagguuau     1440

uuucaggaca uuuaaaaugu agacucaaaa uggauaaauu aagauuaaaa gguguaaguu     1500

auucacuaug cacagcagcu uuuacauuua caaaaguacc agcagaaaca cuacacggga     1560

cagugacagu agaaguacag uacgcaggaa cagaugggcc auguaaagua ccagcacaaa     1620

uggcuguaga caugcagaca uugacaccgg uuggaagauu aauaacagca aauccaguaa     1680

uaacugaaag uacugaaaau ucaaaaauga ugcuagaacu agacccacca uuuggcgacu     1740

ccuauauagu uauaggagug ggugauaaga aaaucacaca ucauuggcau agaucaggaa     1800

guacgauagg aaaagcguuu gaagccacgg uuagaggagc uaaaagaaug gcagugcuag     1860

gagauacggc uugggauuuu ggaaguguag gaggcguguu uaauagccua gggaaaggaa     1920

uacaucaaau auuuggggca gcuuucaaaa gucuauuugg agguaugagu ugguuuaguc     1980

aaauauuaau agguacacua uuaguauggu uaggauugaa cacuaaaaau gggagcauua     2040

guuugacuug uuuagcacua ggugguguaa ugauauuuuu aucaacagca guaagugcau     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  7103
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 2 opt

<400>  7103
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

caauugguau ugucggacua cuccuaacaa cagcaauggc agcagaaauc acucgaaggg      120

gaucggcgua cuauauguau cuugauagga gcgacgcagg aaaagcaauc ucguuugcua      180

caacauuagg aguaaacaaa ugccauguac aaauaaugga uuugggacau augugcgaug      240

caacaauguc cuaugagugu ccuauguugg acgaaggagu ugaacccgac gacguagacu      300

guugguguaa uacgacaagc acauggguug uauaugguac augucaucau aaaaaaggug      360

aagcuagaag aucucggcga gcagugacau uaccaucaca uucaacaagg aaacuacaaa      420

cuagaucaca aacaugguua gaaucacgag aauauacgaa acaucuaauc aaaguagaaa      480

acuggauuuu ucguaaccca ggauuugccc uuguagcagu agcaauagca uggcuucuag      540

gaucgaguac aucccaaaaa guaauuuauu uaguaaugau acuauuaaua gcaccagcau      600

auagcauaag auguauagga guuaguaaca gagacuucgu cgaaggaaug ucaggcggaa      660

cauggguuga uguaguccua gaacacggcg gauguguaac aguaauggcu caagauaaac      720

caacaguaga uauagaacuc gucacgacaa caguaucaaa uauggccgaa guaagaaguu      780

auuguuauga agcgaguauu agugacaugg ccucagauuc aagaugucca acccagggcg      840

aagcauaucu ugauaaacaa ucugacacac aauauguaug caaaagaaca cuuguagaca      900

gaggaugggg aaauggaugc ggacuauuug gaaaaggaag ccuaguaaca ugugcaaaau      960

ucacuuguuc aaaaaagaug acaggaaaaa gcauucaacc ugaaaacuua gaauaucgaa     1020

uaauguuauc uguucaugga ucacaacauu cuggaaugau aguaaaugac acuggacaug     1080

aaacugacga aaacagagca aaaguagaag ucacacccaa uucaccaaga gcagaagcua     1140

cacuaggagg auuugguagu cuaggucuag auugugaacc aagaacugga cuagauuucu     1200

cagacuuaua cuaucugaca augaauaaca aacacugguu aguacauaag gaaugguuuc     1260

augacauacc ucucccuugg cacgcaggag cagauacagg uaccccacau uggaacaaua     1320

aagaagcacu aguagaauuu aaagacgccc augcaaaaag acaaacagua guagucuuag     1380

gaagccaaga aggagcagua cauacagcau uagcaggagc ucuagaagcc gaaauggacg     1440

gagcaaaagg uagacuauuu ucuggucauc uaaaaugccg acuaaaaaug gacaaauuaa     1500

gacuaaaagg uguaucauac agcuuaugca cagccgcauu uacauucacu aaagucccag     1560

cagaaacauu acacgggaca guaacaguag aaguacaaua ugcaggaaca gacggaccau     1620

gcaaaguccc agcacaaaug gcaguagaca ugcaaacauu aacuccagua ggaagacuaa     1680

uaacagcuaa cccaguuauu acagaaucga cagaaaauuc aaaaaugaug cuagaacuug     1740

auccaccauu uggugauuca uauauaguua uaggaguagg agauaaaaaa auaacacauc     1800

auuggcauag aucaggauca acaauaggaa aagcauuuga agcaacagua aggggcgcca     1860

aaagaauggc aguauuagga gacacagcau gggauuuugg uucuguugga gguguauuca     1920

auucauuagg aaaaggaauc caccaaauau uuggagccgc auuuaaaucu cuauuuggag     1980

gaaugucaug guucucacaa auauuaauag gaacauuguu aguauggcua ggauuaaaca     2040

cgaaaaaugg gucaauuagu cuaaccuguc uagcauuggg aggaguaaug auauuucuau     2100

caacagcagu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7104
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 2 opt

<400>  7104
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cuaucgguau aguaggauua cuauuaacaa cggcaauggc cgcagagaua acucgaagag      120

gaucagcgua cuauauguac uuagauaggu cugacgccgg aaaagcaaua aguuuugcua      180

cuacauuagg aguaaauaaa ugucacguac aaauaaugga ucuaggacau augugugacg      240

cuacuauguc auaugaaugc ccaauguuag augaaggcgu ugaaccggau gauguagauu      300

guuggugcaa uacuacauca acauggguag uauauggaac augucaacau aagaaaggug      360

aagcccgaag aagucggaga gcugucacau uaccaucaca cucaacaaga aaauuacaaa      420

caagaucaca aacaugguua gaauccagag aauacacaaa acaucuaaua aaaguugaga      480

acuggauuuu uagaaaccca ggcuuugcuu uaguagcagu ggcaauagcg ugguuauugg      540

gaaguaguac aagucaaaaa gugauauauu uaguaaugau ucuauuaaua gcuccagcau      600

auaguaucag auguauaggu guaucaaaua gagauuuugu agaaggaaug agcggaggaa      660

ccuggguaga ugucguauua gaacauggag gauguguaac uguuauggca caagacaaac      720

cuaccguaga uauagaauua guaacaacua cuguuaguaa uauggcugaa gucagaagcu      780

auugcuacga agcaaguaua agugauaugg caagugauuc gagaugucca acucaaggcg      840

aagcauaucu ugauaaacaa agcgauacac aauauguaug uaaaaggacg uugguagaua      900

gagguugggg aaacggaugu ggcuuauucg gaaaaggaag ucuaguuacu ugcgcgaaau      960

uuacauguag caagaaaaug acagguaaaa guauacaacc cgaaaaucua gaauauagaa     1020

ucauguuauc aguacacgga ucacaacaua guggaaugau aguaaaugau auaggacacg     1080

aaaccgauga aaauagagcc aaaguugaag uaacgccaaa uucgccaaga gcugaagcaa     1140

cauuaggagg auucggaucu cuuggauuag auugcgagcc aagaacagga uuagacuuua     1200

gugauuuaua cuaucuaacu augaauaaca aacauugguu aguacauaaa gaaugguuuc     1260

augauauccc auuaccuugg caugcaggag cagauacagg gacaccucau uggaauaaua     1320

aagaagcauu agucgaguuc aaagacgcuc augcaaaaag acaaaccgua guaguacuag     1380

gaucacaaga aggagccgua cauacggcgc uugcaggagc auuagaagca gaaauggaug     1440

gagcaaaagg aagauuauuu ucuggacauu uaaaauguag auuaaaaaug gauaaacuga     1500

gauuaaaagg aguuucuuau ucuuuaugua cagcaguuug uacagcagcu aaagucccag     1560

cagaaacacu acauggaaca guaacaguag aaguacaaua ugcaggaacc gaugguccau     1620

guaaaguacc agcucaaaug gcagucgaua ugcaaacauu aacuccggua ggaagacuaa     1680

uuacagcuaa cccaguaaua acagagucaa ccgaaaauag caaaaugaug uuagaauuag     1740

auccaccauu uggagauagc uacauuguaa uaggaguugg agauaagaaa auaacacauc     1800

auuggcauag aucgggaagc accauuggga aagcauucga agcgacagug aggggagcaa     1860

aaagaauggc aguauuagga gauacagcgu gggauuuugg aucuguagga ggaguauuua     1920

auaguuuggg aaaaggaaua caucaaauau uuggagcagc auuuaaauca uuauuuggag     1980

gaaugagcug guuuucacaa auauuaauag gcacacuauu ggucuggcuc ggacugaaua     2040

cuaaaaacgg aaguauuagu uuaacgugcc uagcauuagg aggcguaaug auauuccuua     2100

guacagcagu uucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7105
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 2 opt

<400>  7105
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

caauaggaau aguaggcuug uuguuaacaa cagcaauggc agccgaaauu acaagaagag      120

guuccgcaua uuauauguac cuagauagau cagaugcagg gaaagcaaua aguuuugcaa      180

caacuuuagg agucaauaaa ugucauguac aaauaaugga uuugggacau augugugaug      240

caacaaugag cuacgaaugu ccaauguuag augaaggagu ugaaccugac gauguagauu      300

guugguguaa uacaacaagu acauggguag uauacggaac cugucaucau aaaaaaggag      360

aagcucgaag aagcagaagg gcuguaacac uaccgucuca uagcacuaga aaauuacaaa      420

cacgauccca aacauggcua gaaaguagag aauacacaaa acauuuaaua aaaguagaaa      480

auuggauauu uagaaauccc ggauucgcuu uaguagccgu agcaauugca ugguuauuag      540

gaucaucuac aucacaaaaa guaauauauc uuguaaugau acuauuaaua gcgccggcau      600

auaguauuag augcauagga guaaguaaua gagauuuugu agaaggaaug agugguggua      660

cguggguaga ugucguucua gaacacggag gaugcguaac aguaauggca caagacaaac      720

cgaccguaga cauagaauua guuacaacua ccguuucaaa cauggccgaa gugagaucuu      780

acuguuauga agcuucaaua agugauaugg caucagauuc aagaugcccu acacaaggag      840

aagcguaccu ugacaaacaa ucggauacac aauauguaug caaaagaaca cuaguagauc      900

gaggaugggg aaacgguugu ggacuguuug gaaaaggaag uuugguaacu ugugcaaaau      960

uuacauguuc aaaaaaaaug acagguaagu caauacaacc agaaaaccuu gaguauagaa     1020

uaaugcuauc aguacaugga agucagcacu caggaaugau aguaaaugac gaaaacagag     1080

ccaaaguaga aguuacuccc aauaguccac gagcggaagc aacauuggga ggguuuggau     1140

cucuaggucu agauugcgaa ccgagaaccg gauuagauuu cagugauuua uauuauuuaa     1200

caaugaauaa uaaacacugg uuaguucaca aagaaugguu ccacgauauc ccauuaccau     1260

ggcaugcugg agcggauacg ggcacaccac auuggaauaa caaagaagca cuaguugaau     1320

uuaaagaugc acaugcaaaa cgucaaacag uaguaguauu aggaucccaa gaaggcgcug     1380

uacauacagc acuagcaggu gcauuagaag cugaaaugga uggugcuaaa ggacgcuuau     1440

uuucaggaca ucuaaaaugu cgucuaaaaa uggacaaauu aagacuuaaa ggaguaaguu     1500

auagcuuaug uacugcagca uuuacuuuua caaaaguacc agcugaaaca uuacacggaa     1560

cagugacagu ugaaguacaa uaugcuggga cagacggacc auguaaagua ccagcccaaa     1620

uggcaguaga uaugcaaaca uuaacgccug uaggucguuu aauaacugcu aacccuguca     1680

uaacggaauc aacagaaaau ucaaaaauga uguuagaauu agauccaccu uucggagacu     1740

cauacauagu aauaggaguu ggagacaaga aaauaacuca ccacuggcau agaucaggaa     1800

guacgauugg uaaagcauuu gaagcaacgg ugagaggagc aaaaagaaug gcaguauuag     1860

gggauacagc gugggauuuu ggaaguguag gaggaguuuu uaacucauua ggaaaaggua     1920

uacaccaaau auuuggugca gcauuuaaau cucuauuugg agguauguca ugguuuucac     1980

aaauauuaau agguacacua uuaguauggu ugggacuaaa uacaaaaaau ggaucaauau     2040

cucuaacaug cuuagcuuua ggaggaguaa ugauauuuuu aaguacagca gugucagcau     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  7106
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 2 opt

<400>  7106
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacuu       60

caauaggaau aaucggguua cuauuaacaa ccgcaauggc agcugaaaua acccgaagag      120

gaucagcaua cuauauguac cuagauagau cagacgcagg aaaagcaaua agcuuugcaa      180

cgacgcucgg aguaaacaaa ugucaugucc aaauaaugga uuugggacac augugugacg      240

caacuauguc auacgaaugu ccaaugcuug augaaggagu agaaccagau gauguagauu      300

guugguguaa cacaacuuca acauggguag uauacggaac uugucaucau aagaaaggag      360

aaaccagacg aagccgaagg ucaguuaguc uaagauauca cuacacaaga aaacuacaaa      420

ccagaucaca aacauggcua gaaucaagag aauauaaaaa acacuuaauu augguagaga      480

auuggauauu ucguaaucca gguuuugcaa uagugagugu agcaauuacu uggcuuaugg      540

gaaguuuaac uagucaaaaa guuauauauu uaguaaugau aguacuaauc guaccagcau      600

acagcauauc augcauagga guuaguaaca gagaucuagu ugaaggaaug ucaggaggaa      660

cuuggguaga cguuguauua gaacacggag gauguguaac agaaauggcg caagacaaac      720

cgacagucga uauagaauug guaacaauga caguaagcaa uauggcagaa guaagaucau      780

auuguuauga agcaucacua ucugacaugg caucggcauc acgaugccca acacaaggug      840

aaccaaguuu agauaaacaa agugauacac aaagcguuug uaaaagaaca uuaggugaua      900

gagguugggg aaacgguugu ggaauauuug gaaaaggauc acucguaaca uguucuaaau      960

uuaccuguug uaaaaaaaug ccaggaaaau ccauucaacc cgaaaauuug gaauauagaa     1020

uaaugcugcc cguacaugga agucaacaua guggaaugau aguaaaugau auaggacaug     1080

aaacagauga aaaucgugca aaaguugaag uaacaccaaa uucaccuaga gcagaagcaa     1140

cguuaggagg auuuggaagu cuaggcuuag auugugaacc aagaacagga uuagauuuuu     1200

ccgauuuaua uuaucuaaca augaauaaca aacacugguu aguacauaaa gaaugguuuc     1260

augauauacc auuaccaugg caugcgggag cugacacugg aacaccacau uggaacaaua     1320

aggaagcgcu uguagaauuc aaagaugcac acgcaaaaag acaaacagua guaguacuag     1380

gaucacaaga aggagcagua cauacagcau uagccggagc acuagaagca gaaauggacg     1440

gagcaaaagg uagacuauuc aguggucauu uaaaaugcag guuaaaaaug gauaaacucc     1500

guuuaaaagg aguuucuuau aguuuaugua cagcagcauu uacauuuacu aaaguaccug     1560

cagaaacauu acauggaaca guaacuguag aaguacaauc agccggaaca gauggaccuu     1620

gcaaaguacc ugcacaaaug gcaguagaua ugcaaacacu aacaccggua ggaagacuaa     1680

uaacagcaaa ccccguaaua acugaaucaa cagaaaacuc uaaaaugaug cuagaacuag     1740

auccuccuuu uggugacuca uauaucguga uaggcguagg agauaaaaaa aucacacauc     1800

acuggcacag aucaggaucu accauaggaa aagccuucga agcaacagua agaggagcaa     1860

aaaggauggc aguguuaggc gauacagcuu gggauuuugg cagcguaggc ggaguauuua     1920

acucacuugg aaaaggaaua caucaaauau uuggcgcagc auucaaaucc cuauuuggag     1980

gaaugaguug guuuucacaa auauuaauag guacauuacu uguaugguua gguuuaaaua     2040

caaaaaacgg cucaaucagu uuaacuugcc uagcuuuagg aggaguaaug auauuucuaa     2100

gcacagcagu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7107
<211>  2446
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 2 opt

<400>  7107
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagug       60

uuggaauagu aggauuauua cuuacaacag cuauggcagc agaggucaca agacgaggau      120

cagcuuacua uauguauuua gacaggaaug augcugguga agcaauauca uucccgacca      180

cccuggguau gaacaaaugu uacauacaaa uuauggaucu uggacacaug ugugacgcaa      240

cuaugucaua ugaaugucca augcuagaug aaggaguaga accagacgau guagacugcu      300

ggugcaacac aacaagcacu uggguaguau auggcaccug ucaucauaaa aaaggagaag      360

cuagaagaag uagaagagca guaacauuac caagccauuc cacuagaaaa uuacaaacaa      420

gaucacaaac augguuagaa uccagggaau auacaaagca ccucauaaga gucgaaaauu      480

ggauauuuag aaacccagga uucgcccuug cagccgcagc aauagccugg uuacuuggau      540

cgagcacaag ucaaaaaguu aucuaucucg uaaugauauu auuaauagca ccagcauaua      600

gcauacgaug uauuggcgua ucgaauagag auuuuguaga aggaaugagc ggaggaacau      660

ggguagacgu cguucuagaa caugguggau gcguaacagu aauggcacaa gauaaaccua      720

caguugauau agaauuagua acaacaacag uaaguaauau ggcagaagua aggagcuauu      780

gcuacgaagc aucaaucagu gauauggcau ccgauucaag auguccaaca caaggagaag      840

cauacuuaga uaaacaauca gauacacaau auguauguaa acguacacuu guagacagag      900

gauggggaaa cggauguggu cuauucggaa aggguagccu aguaacaugc gcaaaauucg      960

caugcaguaa aaaaaugacu gguaaaagca uacaaccaga aaaucuugaa uauagaauaa     1020

uguuaucagu acauggaagu caacauucug ggaugauagu uaaugauacc ggacaugaaa     1080

cugaugaaaa cagagcaaaa guugaaauua cuccaaauuc accaagagca gaagcaacau     1140

uagguggcuu uggaaguuug ggacuugauu gcgaaccuag aacaggacua gacuuuagug     1200

auuuguauua cuuaaccaug aacaauaaac auugguuagu acauaaagaa ugguuccaug     1260

acauuccauu acccuggcau gcuggagcgg auaccgggac accacauugg aacaacaaag     1320

aagcauuagu agaauuuaaa gacgcacacg ccaaaagaca aacuguagua guacugggaa     1380

gccaagaagg agcaguacau acagcgcuag caggagcauu agaagcagaa auggauggug     1440

caaaaggaag auuaagcuca ggucauuuaa aaugcagacu aaaaauggac aaacuuagau     1500

uaaaaggugu aaguuauuca cuauguacug cagcuuucac auuuacuaaa auaccagcag     1560

aaacacuaca uggcacaguu acuguagaag uccaauacgc aggaacagau ggaccuugua     1620

aaguucccgc acagauggcc guagauaugc aaacacuuac uccaguaggc cgacuuauaa     1680

cagcaaaccc uguaauaaca gaaucaacag aaaauuccaa aaugauguug gaauuagacc     1740

ccccguuugg ugauaguuac auaguaauag gagucgggga aaaaaaaaua acccaucacu     1800

ggcaucgauc cggcagcaca auaggaaaag cauuugaagc cacuguaaga ggagcaaaaa     1860

gaauggcggu auuaggagau acagcauggg auuuuggaag cguagguggc gcacuaaauu     1920

ccuuaggaaa agguauacau caaauauuug gagcugcauu uaaaucucuu uuugggggaa     1980

ugucaugguu uagucaaauu uuaauaggaa cucuauuaau gugguuaggu cucaauacaa     2040

aaaacggauc aauaaguuua auguguuuag cuuugggagg aguccuaaua uuuuuaagca     2100

cggcuguaag cgcaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2160

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2220

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2280

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2400

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2446


<210>  7108
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 2 opt

<400>  7108
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguagguau aguaggacua cuguuaacaa cagcaauggc agcagaagua acuagaagag      120

gaucggcaua uuauauguau cuugaccgga augaugcggg agaagcuaua agcuuuccaa      180

cuacuuuagg caugaauaaa uguuauaucc aaauaaugga ucuaggacau augugugacg      240

caacaaugag uuacgaaugu ccaauguuag acgaaggggu agagccugau gacguagauu      300

gcuggugcaa cacgacaagu acaugggucg ucuauggaac augucaucau aaaaaaggag      360

aagcacgaag aaguaggcga gcaguaacuc uucccucaca caguacacga aaauuacaaa      420

caagaaguca aacaugguua gaaucccgag aguauacaaa acacuugaua agaguagaaa      480

auuggauuuu uagaaaucca ggauuugcac uagcagccgc agcaaucgca uggcuacuug      540

gaagcaguac aagucaaaaa gucauuuauu uagucaugau auuauuaauu gcaccugcau      600

auucaauaag augcaucgga gugucgaaua gagauuuugu agaaggaaug aguggaggaa      660

cauggguaga uguaguauua gaacauggag gcuguguaac ugucauggcc caagauaaac      720

ccacaguaga uauagaauua guaacgacaa caguaucaaa uauggcagaa guaagaucau      780

acuguuauga ggcuaguauu ucagacaugg caagugauuc uagauguccc acgcaaggug      840

aagcauaucu agacaagcaa ucagauacac aauauguaug uaaaaggaca uuaguggauc      900

gaggcugggg aaaugguugu ggacuuuuug ggaaagguuc auuagucaca ugugcaaaau      960

uugcguguuc aaaaaaaaug acaggaaaaa guauccaacc agaaaacuua gaauacagaa     1020

uaaugcuauc uguacacgga ucucaacaua guggcaugau aguaaaugau acgggacaug     1080

aaacugacga aaacagagca aaaguagaaa uaacaccaaa cucaccuaga gccgaagcaa     1140

cauuaggcgg auuuggaucu cuaggauuag auugcgaacc aagaacugga uuagauuucu     1200

cagauuuaua uuaucuaaca augaauaaca aacauugguu gguacauaaa gaaugguuuc     1260

acgacauccc auuaccaugg caugcaggag ccgauacugg aacaccacau uggaacaaua     1320

aagaagcacu uguugaauuu aaagaugcac acgcaaaacg acaaacuguu guaguucuag     1380

gaucgcaaga aggugccgua cauacagcgc uugcaggagc acucgaagca gaaauggaug     1440

gcgcaaaagg aagauuaagu ucagggcauc uuaaaugcag acuaaaaaug gauaaauuaa     1500

gguuaaaagg aguuucauau aguuuaugca cagcagcauu uacauuuacu aaaauaccag     1560

cggaaacauu gcacggaaca gugaccguag aagugcaaua ugccggaaca gacggucccu     1620

guaaaguacc agcucaaaug gcuguagaca ugcaaacguu aacuccaguc ggaagauuaa     1680

ucacagcgaa cccaguaaua acagaaagua cagaaaauuc aaaaaugaug uuagaauuag     1740

acccaccauu uggugauagc uauauaguaa uaggaguagg ugaaaaaaaa auaacccauc     1800

auuggcauag gucuggaagu acaauaggua aagcauucga agcaacagua agaggagcua     1860

gaagaauggc uguauuggga gauacugcgu gggacuuugg aucaguaggg ggagcccuua     1920

auucacuagg aaaaggaaua caucaaauuu uuggagcagc auucaaauca cuauuuggag     1980

guaugucaug guuuagccaa auucuaauag gaacauuauu aauguggcuu ggauuaaaca     2040

caaaaaaugg aaguauuuca cuaaugugcc uagcacucgg uggaguguua aucuuuuuau     2100

caacagcagu aucugcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7109
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 2 opt

<400>  7109
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacuu       60

caguagguau aguaggauua cuuuuaacua ccgcgauggc agcagaagua acaagaagag      120

ggucagcuua uuacauguac uuagacagaa augaugcagg agaagcaauc aguuucccaa      180

cuacauuagg uaugaacaaa uguuacaucc aaauuaugga uuuaggacau augugugacg      240

caacaauguc cuacgaaugu ccaauguuag augaaggagu ugaacccgau gacgucgacu      300

guuggugcaa uacaacaagu acuuggguag ucuaugggac augccaucau aagaaaggag      360

aagcgagaag aagucgacga gcaguaacac uaccaucuca uaguacaaga aaauuacaaa      420

cacgaaguca aacauggcua gaaucuagag aauauacaaa acaucuaauc agaguagaaa      480

auuggauguu ucgaaauccu ggauuugcac uagccgcagc agcaauagca uggcuauuag      540

gaucaucaac aucacaaaaa guaauuuacu uaguaaugau uuuguuaaua gcuccugcau      600

auaguauaag augcauagga guaucaaaca gagauuuugu agaaggaaug ucaggaggua      660

cauggguaga uguaguuuua gaacauggcg gauguguaac aguuauggca caagauaaac      720

caacuguaga uauagaauua guaacaacua caguaucuaa cauggcugaa guuagaucgu      780

acuguuacga agcaagcauu ucugacaugg caucagauuc uagauguccu acacaaggag      840

aagcauaucu ugauaaacag ucagauacac aauacguaug uaaaagaaca uuaguagaua      900

ggggaugggg aaauggaugc ggucuauuug ggaaaggauc guuaguuaca ugugcaaaau      960

ucgcauguag uaagaaaaug acaggcaaau caauacaacc cgagaaccuu gaauauagaa     1020

uaauguuaag cguucaugga ucccaacaca gcggaaugau agucaacgau accggucacg     1080

aaacugauga aaaccgugca aaaguagaga uaacuccaaa cucaccacgu gcagaagcaa     1140

cauuaggagg uuuuggcucu cuaggacucg acugugaacc cagaacuggu cuagacuuuu     1200

cagaucuaua uuauuugaca augaauaaca aacauuggcu gguucauaaa gaaugguucc     1260

augauauacc auuaccaugg caugccggag cagauacagg aacuccgcac uggaacaaua     1320

aagaggcuuu aguagaauuu aaagaugccc augcaaaacg acaaacagua guuguauuag     1380

gaagucaaga aggugcaguu cacacagcuc uggcaggagc auuagaggca gaaauggacg     1440

gcgcuaaagg uagauuauca uccggacauu uaaaauguag auuaaaaaug gauaaauuaa     1500

gauuaaaagg uguaagcuau agccuaugca cugcugcauu uacauucaca aaaauaccag     1560

cagaaacauu acauggaaca guuacgguug aaguucaaua ugcagguacu gacggaccau     1620

guaagguacc ugcacaaaug gcaguggaua ugcaaacauu gacucccguu gguagguuaa     1680

uaacagcaaa uccagucaua acugaaucaa cagaaaauuc aaaaaugaug cuagaauuag     1740

auccuccauu uggagacagu uacauaguaa uaggaguagg ugaaaagaaa auaacacauc     1800

auuggcacag aagcggaagu acuaucggaa aagccuuuga agccacgguu agaggugcaa     1860

aaagaauggc gguauuggga gauacagcau gggacuucgg caguguagga ggagcuuuaa     1920

auucauuagg aaaaggaaua caccaaauau ucggagcugc auuuaaaagc cucuucggag     1980

guaugucaug guuuucccaa auucuaauag gaacacuacu aaugugguua ggacuaaaua     2040

cuaaaaaugg aucaauauca cuaaugugcu uagcacucgg aggaguacua aucuuccuau     2100

caacagcagu aagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7110
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 2 opt

<400>  7110
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguagguau aguaggacua uuauuaacaa cagcuauggc agcagaagua acgcggagag      120

ggucagcgua uuauauguau cucgauagaa acgaugccgg agaagcaaua agcuuuccaa      180

caacauuggg aaugaauaaa uguuauauac aaauaaugga uuuaggacau augugugaug      240

caaccauguc auacgagugc ccaauguuag augaaggugu agaaccagau gauguugauu      300

guuggugcaa caugacaagu acauggguag ucuauggaac augucaucac aaaaaaggag      360

aagcaagaag aucaagaaga gcaguaacac uacccuccca uucaacgaga aaacuucaaa      420

caaggaguca aacaugguua gaaucccgag aauacacaaa acaucuaaua agaguagaaa      480

auuggauauu ucgaaaucca gguuuugcuc uagcugcagc agcgauagca ugguuacuug      540

gaagcucuac cucacaaaaa guaauauauu uaguuaugau ucuacuaaua gcaccagcau      600

auucaauaag augcauagga guaucaaauc gagauuuugu cgaaggaaug ucaggaggaa      660

cauggguaga uguuguccua gaacauggag gguguguuac uguaauggca caagauaaac      720

cuacaguaga cauagaacuu guaacaacaa caguaucaaa uauggcugaa guucguucau      780

auuguuauga agcuaguaua agcgacaugg ccagcgauuc aagaugucca acacaaggag      840

aagcauacuu agauaaacaa agugauacac aauauguaug caaacgaacu cuuguugaua      900

gaggaugggg uaaugguugc ggauuauuug gaaaaggauc acuuguaaca ugugcaaaau      960

uugcauguag uaagaaaaug acgggcaaau caauacaacc cgaaaauuug gaauauagaa     1020

uaauguuaag uguucaugga agccaacacu caggaaugau aguaaaugau acaggacaug     1080

aaacagauga aaaucgagca aaaguagaaa uaaccccuaa cagcccacga gcagaagcaa     1140

cguuaggagg auuuggauca cuaggccuag auugugaacc acgaacugga cuugacuuua     1200

gcgauuuaua cuaccugacu augaacaaca aacacugguu aguacauaaa gaaugguuuc     1260

augacauacc acuuccuugg caugcaggug cagauacagg aacaccacau uggaauaaua     1320

aagaagcuuu aguagaauuu aaagaugcgc augcaaaacg ccaaacaguu guaguauuag     1380

gcucccaaga aggagcggua cauaccgccc ucgcaggagc auuagaagca gaaauggaug     1440

gagccaaagg uagauuauca ucuggccauu uaaaauguag guuaaaaaug gacaaauuac     1500

gauuaaaagg uguaaguuau ucauuaugca cagcugcauu cacauucaca aaaauuccag     1560

cagaaacauu acacggaaca guaacaguug aaguucaaua cgcgggaacc gacggaccau     1620

guaagguacc agcccaaaug gccguagaca ugcaaacauu aacaccagua ggaagauuaa     1680

uaacagcuaa uccaguaaua accgaaagca cagaaaauuc aaaaaugaug uuagaauuag     1740

auccuccauu uggagauucg uauaucguga uugggguagg agaaaaaaaa auuacacacc     1800

acuggcauag aucaggcuca accauaggaa aagcauuuga agcuacugua agaggugcua     1860

aaagaauggc aguucucggu gacaccgcau gggacuuugg aaguguaggu ggugcauuaa     1920

auucauuagg aaaaggaauc caccaaauau uuggagcagc auuuaaaucg uuauucggag     1980

gcaugucaug guuuagucaa auauuaauag guacuuuauu aaugugguua ggacuaaaua     2040

caaaaaaugg uaguauaagu uuaauguguu uagcucuagg aggaguauug auauucuuau     2100

caacugcagu uagcgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7111
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 2 opt

<400>  7111
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguaggaau aguuggucua uuacuaacaa cagcuauggc agcagaagua acaagaaggg      120

gaucagcaua uuauauguau uuagaucgaa augaugcugg agaagccaua ucuuuuccca      180

caacacuagg aaugaacaaa ugcuauauac aaauuaugga uuuaggucau augugugaug      240

caacaauguc auacgaaugu ccuauguuag augaaggagu agaaccggau gauguagauu      300

guugguguaa uacaaccuca acauggguag ucuacgguac uugucaucau aaaaaaggug      360

aagcaagaag gaguaggaga gcgguaacuu uaccaucgca uucaacaagg aaacuucaaa      420

caagaaguca aacauggcua gaaaguagag aauauacaaa acaucucaua agaguugaaa      480

acuggauauu cagaaauccu ggcuuugcuu uggcugcagc agcaauagcg ugguuacuag      540

ggucaucaac aucgcaaaaa guuauauacc ugguuaugau auuauuaaua gcaccggcau      600

auaguauuag auguauagga guaucaaaua gagauuuugu ugaaggaaug agcggcggaa      660

cauggguaga cguuguucua gaacauggag gaugcguaac agcuauggcu caagauaaac      720

cgacaguaga cauagaauua guaacaacua caguuagcaa uauggcugaa gucaggucau      780

acuguuacga agccagcaua agugauaugg cuagcgacag uagauguccc acucaaggag      840

aagcauaucu agauaaacaa agcgauacac aauaugugug uaaacguaca uuaguagaca      900

gaggaugggg caauggaugc ggauuauuug gaaaagguag ucuaguaaca ugugcaaaau      960

uugccugcag caaaaaaaug acaggaaaaa guauacaacc agaaaacuua gaauaccgaa     1020

uaauguuauc aguacauggu agucaacaca guggaauguu aguaaacgau acaggucaug     1080

aaacagauga aaaucgugca aaaguagaaa uaacaccaaa uucgccaagg gcagaagcaa     1140

cucuaggagg auuuggauca cuagguuugg auugugaacc aaggacagga cuagauuuuu     1200

cagauuuaua uuaucuaacu augaauaaua aacauugguu agcacauaaa gaaugguuuc     1260

acgauauacc auuaccaugg cacgcgggag cagcaacugg cacaccacau uggaauaaua     1320

aagaagcauu aguagaauuc aaagaugcac acgcaaaaag gcaaacugua guaguacuag     1380

gcucacaaga aggagccgua cauaccgccu uagcaggagc auuagaagcu gaaauggaug     1440

gagcaaaagg aagauuaucu ucuggacauc uaaaaugucg auuaaaaaug gauaaacuaa     1500

gauugaaagg aguaagcuac uccuugugua cagcugcauu uacauuuaca aagaucccag     1560

cugaaacagu ugauggaacu guaacuguag aaggacagua uggaggaaca gaugggccuu     1620

guaaaguucc agcacaaaug gccguagaua ugcaaacguu aacuccuguu ggaagacuaa     1680

uaacagcaaa uccgguaauc acagaaagca cagaaaauag caaaaugaug uuagaauuag     1740

acccaccuuu uggagauuca uauauaguaa uugguguagg agaaaaaaaa auaacacacc     1800

acuggcauag aucuggaagu acaauaggaa aagcauuuga agcaaccgua aggggagcga     1860

aaagaauggc aguucuaggc gauacagccu gggacuuugg aagcgucgga ggcgcauuaa     1920

auaguuuagg aaaaggaaua caucaaauua uaggagcugc cuucaaauca uuauucggag     1980

gcaugucuug guuuagucag auauuaaucg guacauuacu aaugugguua ggauuaaaua     2040

caaaaaacgg uagcauauca uuaauguguc uagcguuagg aggaguuuua auuuuucuaa     2100

guacagcagu aucaggguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7112
<211>  2440
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 2 opt

<400>  7112
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggggg       60

cagacgcuuc uauaggaaua guaagccuau uauuaacuac uguuauggca gcagaaauaa      120

caagaagggg uagugccuau uauauguauu uagacagaag ugaugcagga aaagcaauaa      180

guuuugucac aacuuuagga guaaauaaau gucacguuca aauaauggau uuaggacaua      240

ugugcgaugc gacaaugagu uaugaauguc cuauguuaga ugaaggcgua gaaccagaug      300

acguagauug uugguguaau accacaucaa cuuggguugu auauggaacu ugucaucaca      360

aaaaaggaga agcgagaaga ucaaggagag caguaacauu accaagucau ucaacaagaa      420

aacuucaaac aagaagccaa acaugguuag aaaguagaga guacacuaaa caucuaauaa      480

aaguagaaaa uuggauauuu agaaauccag guuuugcacu aguugcaguc gcuauagccu      540

ggcuacuagg aucaucuaca agccaaaaag uaaucuaucu aguaaugauc uuacuaauag      600

caccagcaua uaguauaaga uguauuggag uaagcaauag agauuuugua gaaggaauga      660

gcggaggaac uuggguugac guaguauuag aacauggcgg uugcguuaca gucauggcac      720

aagauaaacc aacaguagau auugaacuag ugacaacaac agugagcaau auggcagaag      780

uaagaucgua uuguuaugaa gcaucaauau cugauauggc uucugauagc agaugcccca      840

cacaagguga agcauauuua gacaaacaaa gugacacaca auauguaugu aaaagaacau      900

uaguagacag aggaugggga aacggaugug gauuguuugg aaaagguagu uuaguuacau      960

gugcaaaauu cacuuguuca aaaaaaauga caggaaaauc uauccaacca gaaaauuuag     1020

aauacagaau uaugcuaagu guucauggaa gucaacacuc gggaaugaua gucaaugacg     1080

aaaacagggc caaaguugaa guuacgccaa acucaccuag agcagaagcc acacuaggag     1140

gauuuggauc acuaggacuu gacugugaac ccagaacggg acuagauuuu agugauuuau     1200

auuaucuuac aaugaauaac aaacauuggc uaguacacaa agaaugguuu caugacauac     1260

cacuacccug gcacaguggu gcagauaccg aaacaccaca uuggaacaau aaagaagcac     1320

uaguagaauu caaagaugca caugcaaaga gacagaccgu gguuguacua gggagccagg     1380

aaggagcagu acacacggca uuagcgggag cauuagaagc agaaauggac ggagcgaaag     1440

gaagauuauc aagcggacau cuaaaaugua gauuaaaaau ggauaaauug agauuaaaag     1500

gaguuucaua caguuuaugu accgcugcau uuacuuuuac uaaaguuccc gcugaaacgc     1560

uacacggaac aguaacagua gaaguucaau augccggaag agacgguccc uguaaaguac     1620

ccgcacaaau ggccguagau augcaaacac uaacaccggu gggaagacua auaaccgcca     1680

acccaguuau uacagaauca acagagaauu caaaaaugau guuagaauua gaucccccau     1740

uuggugauuc uuauauagua auaggaguag gugauaaaaa aauaacccac cacuggcaua     1800

ggagcggauc aauaauaggc aaagcauucg aagcaacagu ccgaggagca aaaagaaugg     1860

caguacuugg ugauaccgca ugggacuuug gaaguguagg aggaguauuu aauagucuag     1920

guaaaggaau ucaucaaauu uuuggagcgg cuuuuaaguc auuauucgga ggaauguccu     1980

gguucaguca aauccuaaua ggaaccuugc uaguaugguu aggauuaaau acaaaaaaug     2040

gcucaauuuc auuaacaugu cuagcacuug gcggaguaau gauauuuuua uccacugcag     2100

uaucggcaug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2160

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2220

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2280

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2400

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2440


<210>  7113
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 2 opt

<400>  7113
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggag       60

ccgacacuag uauaggaaua auaggauuau uauuaacuac agcaauggcg gcugaaauaa      120

cucgaagagg aucagcauau uauauguauu uagauagauc agaugcugga aaagcaauaa      180

guuuugcaac aacaauggga guaaacaaau gccauguaca gauuauggau cuaggucaca      240

ugugugaugc uacaaugagu uaugaaugcc caauguuaga ugagggagua gaaccagaug      300

auguggauug uugguguaau acaacgucaa cauggguugu auauggaaca ugucaccaca      360

aaaaaggaga agcaaggccg ucaagacgag caguaacauu accuagucau aguaccagaa      420

aacuacaaac uagaagccaa acaugguuag aaaguagaga auauacaaaa cacuuaauaa      480

aaguggaaaa uuggauacua aggaauccag gcuuuacugu agcggcauua gcuauggcau      540

ggcuacuugg gaauucaaca ucacaacgag uaauauauuu aguaaugaua uuacuaauag      600

cgccugcaua uuccauaaga ugcauuggag uaagcaaccg cgacuuugua gagggaaugu      660

caggcggaac uuggguugau guaguauuag aacacggugg auguguaacu guaauggcac      720

aagacaaacc uacaguagac auugaauuag uuacgacaac aguaagcaau auggcagaag      780

uacguuccua uuguuaugaa gcuaguauau cagauauggc aagcgauucu agauguccua      840

cacagggaga agcauauuua gacaaacaaa gugauacaca auacguaugc aaacgcaccc      900

uaguagaucg aggaugggga aacgggugcg gauuauuugg uaaaggauca uugguuacuu      960

gcgcgaaauu uacauguagu aaaaaaauga caggaaaauc aauucaaccu gaaaauuuag     1020

aauaucgaau aaugcuguca guacauggau cacaacauag uggcaugaua gucaaugaua     1080

uaggacauga aacagaugaa aacagggcca aaguagaagu cacccccaau ucccccagag     1140

cagaagcgac auuaggcggc uuuggaaguu uaggauuaga uugcgaaccc agaacaggau     1200

uagauuuuuc ugauuuauau uaucuaacaa ugaacaacaa acacugguua guacacaaag     1260

aaugguuuca cgacauaccc cuaccguggc augcaggagc agauaccgga acaccacauu     1320

ggaauaauaa agaagcauua guagaauuua aagaugcuca cgcaaaaaga caaacaguug     1380

uaguauuagg aucucaagaa ggugcuguac auacagcacu ugcaggagca uuagaagcag     1440

aaauggaugg agcaaaagga agauuauuuu ccggacaccu uaaaugcaga auaaaaaugg     1500

acaagcuaag auuaaaggga guaaguuauu cacuauguac ugcagcuuuu acauuuagua     1560

aaguaccagc agaaacuuua caugguaccg uaacuguuga aguucaauau gcaggaaggg     1620

augggccaug caaaguaccu gcccaaaugg caguagauau gcaaacuuua acgccuguag     1680

guagauuaau uaccgcaaau ccuauuauua cagaaucaac ugaaaauagc cgcaugaugu     1740

uagaacuaga uccgccauuu ggugauagcu auauaguuau aggaguagga gauaaaaaaa     1800

uaacacauca uuggcauaga aguggaucaa ccauaggcaa agcauuugaa gcaacaguaa     1860

gaggagcgaa aagaauggca guacuaggcg auacugcaug ggacuuugga ucaguaggug     1920

gaguauucaa uucauuagga aaaggaguac aucaagucuu uggagcugcu uucaaaaguu     1980

uauuuggagg gaugucaugg uuuagccaaa uauuaauugg gacuuuauua guuuggcugg     2040

gauuaaauac aaaaaaugga aguauaucau uagcuugucu ugcacuagga ggagcaauga     2100

uauuccuaag cacagcugua ucagcaugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  7114
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 2 opt

<400>  7114
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auauuuagua augauacuau uaauagcacc agcuuauagc auaagaugua      120

uaggaguuuc aaacagggau uucgucgaag gaaugagugg uggaacaugg guagauauag      180

uucuagaaca uggagguugu guaacaguga uggcacaaga uaaaccaacu guagauauag      240

aauuaguuac uacaaccgua ucaaauaugg cagaaguuag aucauauugu uaugaagcau      300

caauaaguga uauggccuca gauucaagau guccaacaca gggggaagcc uacuuagaua      360

aacaaucaga uacucaauau guauguaaaa gaacauuagu agauagaggu uggggaaaug      420

gauguggauu auuuggaaaa gguaguuuag uaacgugugc aaaauucgca uguucuaaaa      480

aaaugacagg aaaaaguaua caaccugaaa accuagaaua uagaauaaug cuaucaguac      540

augguaguca acauucggga augauaguaa augauacagg acaugaaaca gacgaaaaua      600

gagcaaaagu ugaaauaacc ccaaauaguc caagagcaga agcaacauua ggaggauuug      660

gaucauuagg acuagauugc gaaccaagaa caggauuaga uuuuagugau uuauauuauc      720

uaacaaugaa uaauaaacac ugguuaguac auaaagaaug guuccaugau auaccacuac      780

cauggcacgc aggugcagau acaggaacac cacauuggaa uaauaaagaa gcauuaguug      840

aauucaaaga cgcccaugca aaaagacaaa caguaguagu acuugguagu caagaaggug      900

cuguucacac agcauuggca ggagcauuag aagcagaaau ggauggagcu aaaggaagau      960

uaucaucagg acauuuaaaa ugcagauuaa aaauggauaa acuaagacua aaaggaguaa     1020

guuauagucu augcacggcg gcauuuacau ucacuaaaau accagcggaa acacuacaug     1080

ggacaguaac aguagaagua caauaugcag gaacagaugg accuuguaaa guuccagcuc     1140

aaauggcagu agauaugcaa acauuaacac cgguagguag auuaauaaca gcuaacccag     1200

uaauaacaga aaguacugaa aauucaaaaa ugaugcuaga acuagaucca ccauuuggag     1260

acucauauau aguaauagga guaggagaaa aaaaaauaac ucaucauugg cacagaucag     1320

gaaguacaau aggaaaggca uucgaagcaa caguaagagg agcuaaaaga auggcaguuu     1380

uaggagauac agcaugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7115
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 2 opt

<400>  7115
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auauuuagua augauacuac ucauagcacc agcuuauagu auaagaugca      120

uuggaguauc aaaucgagau uuuguagaag guaugucagg aggaacaugg guagauguag      180

uacuagaaca ugguggaugu guaacuguaa uggcacaaga caaaccaaca guagacauag      240

aauugguaac aacaacagua aguaauaugg cagaaguaag aucauauugu uaugaagcaa      300

gcauaucaga uauggcaagu gacagcaggu guccaacuca aggugaagca uaccuagaua      360

aacaaucaga uacacaauau guuuguaaaa gaacauuagu cgacagaggu uggggaaaug      420

guugcggacu auucggaaaa ggaagcuuag uaacaugugc aaaauuugca uguagcaaaa      480

aaaugacagg uaaaucaauu caaccagaaa auuuagaaua cagaauaaug cuuaguguac      540

acgguuccca acauagugga augaucguaa augacaccgg acaugaaacu gaugaaaauc      600

gugcaaaagu agaaauaaca ccaaauuccc caagagcuga agcaacacua ggaggauucg      660

guucauuagg auuagauugu gaaccuagaa caggauuaga uuuuucagau cuauauuauc      720

uaacgaugaa uaauaaacau ugguuaguac auaaagaaug guuucaugau auaccauuac      780

ccuggcaugc uggagcagau acagggacac cacauuggaa uaauaaagaa gcguuaguug      840

aauuuaaaga cgcacaugca aaaagacaaa caguaguagu acuuggauca caagaaggcg      900

caguacauac agcacuagca ggagcacugg aagcugaaau ggauggugca aaggguagac      960

uaucaagcgg acaucuaaaa uguagacuaa aaauggauaa acuaagacua aaaggaguuu     1020

ccuauaguuu guguacagca gcauuuacuu uuacaaaaau accagcagaa acauuacaug     1080

gaacaguaac aguagaaguu caauaugcag gaacggaugg accauguaaa guaccagcac     1140

aaauggcagu agacaugcaa acauuaacac caguaggaag auuaauaacc gcuaauccag     1200

uaauaacaga aucaacagaa aauucaaaaa ugaugcuuga acuugaucca ccauuuggag     1260

auucauacau aguaauagga guaggagaaa aaaaaauaac acaucacugg cacagaagug     1320

gaucaacaau aggaaaagca uuugaagcaa cgguaagagg agcaaaaaga auggcaguau     1380

uaggagauac ugccugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7116
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 2 opt

<400>  7116
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucuc       60

aaaaaguaau auauuuaguu augauauuau uaauagcacc agcauauuca auaagaugua      120

uaggaguauc aaauagagac uuuguagaag gaaugagugg aggaacaugg guagauguag      180

uacuagaaca uggaggaugu guaacaguua uggcacaaga uaaaccaacg guagacauag      240

aauuaguaac aacuacagua ucaaauaugg cagaaguaag aagcuacugu uaugaagcau      300

caauuaguga cauggcaagu gacagcagau guccaacaca aggagaagca uauuuagaua      360

aacaaaguga uacacaauac guaugcaaaa gaacauuagu agacagagga uggggaaaug      420

gauguggauu guuuggcaaa ggaaguuuag uaacaugugc gaaauuuacu uguaguaaga      480

aaaugacagg gaaaucaaua caaccagaaa auuuagaaua uagaauaaug cuaaguguac      540

augguucaca acacucaggg augauugguu acgaaacgga ugaagauaga gcaaaaguag      600

aaguuacacc aaauagcccc agagcagaag caacguuagg uggcuuuggc aguuuaggau      660

uagauuguga accaagaaca ggauuagacu uuagugaucu auauuauuua acaaugaaua      720

auaaacauug guuaguacac aaagaauggu uccaugauau accucuacca uggcaugcag      780

gagcagauac aggcacaccg cacuggaaua auaaagaagc auuaguagaa uuuaaagaug      840

cacaugcaaa acgacaaacc guagucguau uaggaaguca agaaggggca guucacacag      900

cauuagcagg agcucuugaa gcagaaaugg auggagcaaa aggaagauua uucucaggac      960

acuuaaaaug uagacuaaaa auggauaaau ugagacucaa agguguauca uauagucugu     1020

guacagcagc auucacauuu acaaaaguac cugcagaaac auuacauggg acaguaacag     1080

uugaaguaca auaugcaggu acugauggac cauguaaaau cccuguacaa auggcagugg     1140

auaugcaaac ucuaacacca guaggaagac uaauaacagc uaauccagua auaacagaaa     1200

gcacagaaaa uagcaaaaug auguuagaau uagauccacc cuucggagau aguuacauag     1260

uaauaggagu uggagauaag aaaauaacac accacuggca uagaucaggc aguacaauag     1320

gaaaagcauu ugaagcuaca gugagaggug cuaaacgaau ggcuguauua ggagauacag     1380

cuugggauuu uggaaguuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  7117
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 2 opt

<400>  7117
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguuau auaucuaguu augauacuau uaauagcccc agcauauagu auaaggugua      120

uaggggugag uaacagagau uucguagaag gaaugucagg aggaacaugg guagauguag      180

uacuagaaca cggaggaugu guuacaguaa uggcacaaga uaaaccaaca guagauauag      240

agcuaguaac gacaacagua aguaauaugg cagaaguucg aucuuauugu uaugaagcaa      300

guauaaguga uauggcaagu gauucacguu guccaacaca aggagaagca uauuuagaua      360

aacaaaguga uacucaauau guuugcaaaa gaacauuagu agauagagga uggggaaacg      420

ggugugguuu auuugguaaa ggaaguuuag uaacaugcgc aaaauuugca uguagcaaga      480

aaaugacugg aaaaaguaua caaccagaaa auuuagaaua uagaauaaug cuaagcguac      540

acgguaguca acauaguggu augauuguca augauacugg acaugaaaca gacgaaaaua      600

gagcuaaagu agaaauaaca ccaaauagcc cacgagcaga agcuacacuc ggaggauuug      660

guucuuuagg auuggauugu gaaccucgaa ccggacuaga uuuuagcgau cucuacuacu      720

uaacaaugaa uaauaaacau uggcuaguac auaaagaaug guuucaugau aucccauuac      780

cauggcaugc aggagcagau acaggaacac cacauuggaa caauaaagaa gcauugguag      840

aauuuaaaga cgcacaugca aaaagacaaa cuguaguagu acuaggauca caagagggug      900

cuguacauac agcgcuagca ggcgcacuag aagcagaaau ggauggugca aaagggcgau      960

uaaguucagg acaucuaaaa uguagauuaa aaauggauaa auuaagacua aaaggaguau     1020

cauauucauu auguacugcu gcauuuacau uuacaaaaau accggcagaa acauuacaug     1080

gaacaguaac aguagaagua caauaugcag gaacagacgg accauguaaa gucccagcac     1140

aaauggcagu ugauaugcaa acauuaacac cuguaggaag auuaauaaca gcaaauccag     1200

uaauaacaga aucaacagaa aacucaaaaa ugauguuaga auuagauccu ccauuuggug     1260

acagcuauau aguaauaggu guaggagaaa agaaaauuac acaccauugg cauagaagcg     1320

gaucaaccau aggaaaagca uuugaggcaa caguaagagg agcaaaaaga auggcaguau     1380

ugggagacac agcaugggau uucggaaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7118
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 2 opt

<400>  7118
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau auauuuagua augauauuac uuauagcucc agcuuauagu auaagaugua      120

uaggggucuc aaauagagac uuuguugaag gaaugagugg aggaacaugg guagacguag      180

uuuuagaaca uggaggaugu guaacuguaa uggcacaaga uaaaccgaca guagauauag      240

aauuaguaac aacaaccgua aguaauaugg cagaaguaag aagcuauugc uaugaagcaa      300

gcaucaguga uauggcaagu gauaguagau guccaacaca aggagaagca uaccuagaca      360

aacaaaguga cacacaauau guauguaaaa gaacacuagu agauagagga uggggaaacg      420

gaugcggauu auuugguaaa gguucauuag uaacaugugc aaaauuuaca uguagcaaaa      480

aaaugacagg aaaaagcauu caaccagaaa aucuagaaua cagaaucaug cuaucuguac      540

auggaucaca acacaguggu augauaguua augauacugg auaugaaaca gaugaaaaua      600

gggcaaaagu ggaaguaacu ccaaauagcc caagagcaga agcuacauua ggaggauucg      660

gaaguuuagg acuagauugc gaaccaagaa caggacuaga cuuuucagau cuauauuauu      720

uaacuaugaa uaauaaacau ugguuaguac acaaagaaug guuucaugau auaccauuac      780

cauggcacgc aggagcagau acuggaacac cccauuggaa uaauaaagaa gcacuaguag      840

aauucaaaga ugcucaugca aaaagacaaa cuguaguagu auuaggcuca caagaaggag      900

ccgugcauac cgcauuagcu ggagcucuag aagcugaaau ggauggagca aaagggaaau      960

uauucagugg acacuuaaaa ugucgacuaa aaauggauaa guugaggcua aaagguguaa     1020

guuauucauu auguacagca gcauucacau uuacuaaagu accagcagaa acauugcaug     1080

gaacgguaac aguggaaguc caauaugcag gaacggaugg accaugcaaa auaccaguac     1140

agauggcagu agauaugcag acauuaacac caguaggaag acuaauaaca gcgaauccag     1200

uaauaacaga aaguacagaa aauaguaaaa ugauguugga auuagauccc cccuuuggag     1260

auucauacau aguaauagga guaggagaua aaaaaauaac ucaccauugg cauaggagug     1320

gaucaacaau cggaaaagca uuugaagcaa cuguaagagg agcaaaaagg auggcaguac     1380

uaggagauac agcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7119
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 2 opt

<400>  7119
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaagucau auauuuagua augauauuau uaauugcacc ugcauauagu auaagaugua      120

uaggaguaag uaauagagac uuuguagaag gaaugagugg uggaacaugg guagacguag      180

ucuuagaaca uggaggaugu guaacuguaa uggcacaaga caaaccaaca guugauauag      240

aauuaguuac cacaacagua aguaauaugg cagaaguaag gaguuauugu uaugaagcaa      300

guaucaguga cauggcaagu gauagucgau guccaacaca aggagaagca uaucuagaua      360

aacaaucaga uacacaauau guauguaaaa gaacauuagu agauagagga ugggguaacg      420

guuguggacu auuugguaaa ggaagcuuag uaacuugugc aaaauucaca uguucaaaaa      480

aaaugacagg aaaaucaaua caaccagaaa aucuggaaua cagaauaaug uuaucaguac      540

auggaaguca acauagugga augacaguaa acgauauagg auaugaaaca gaugaaaaua      600

gagcaaaagu agaaguaacu ccaaauagcc caagggcaga agcaacacua ggagguuuug      660

gaucacuggg auuagauugc gaaccaagaa cuggacucga cuucagcgau uuauacuauu      720

uaacuaugaa uaauaaacau ugguuaguuc auaaagaaug guuucacgac aucccauuac      780

cauggcacgc aggagcggau acagguacac cacacuggaa caauaaagag gccuuaguag      840

aauuuaaaga ugcacaugca aaaaggcaaa cuguaguagu auugggaagu caagagggag      900

caguacauac agcacuagca ggugcauuag aagcagaaau ggacggagca aaagguaaac      960

uauuuucagg acaucuaaaa ugcagauuaa aaauggacaa auuacguuua aaaggaguaa     1020

guuacucacu uuguacagcg gcuuuuacau ucacaaaagu accagcagaa acauuacaug     1080

gcacaguuac aguagaagua caauaugcag gaacagacgg accauguaaa auaccaguac     1140

aaauggcagu agacaugcaa acacuaaccc cgguaggaag acuuauaaca gcaaauccag     1200

uaauaacaga aaguacugaa aauucaaaaa ugaugcuaga auuagauccu ccauuuggag     1260

auucauacau aguaauaggu guaggggaua aaaaaauuac acaccauugg cauagauccg     1320

gaagcacaau aggaaaagca uuugaagcaa cgguaagagg agcuaaaaga auggcuguac     1380

uaggagauac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7120
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 2 opt

<400>  7120
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguuau auaucuagua augauauuau uaauagcacc agcuuauagu auaagaugua      120

uaggaguauc aaacagagau uuuguagaag gaaugagcgg agggacaugg guagacguag      180

uauuagaaca uggagguugu guaacaguaa uggcacaaga uaaaccaacg gucgauauag      240

aauugguaac gaccacggua ucaaauaugg cagaaguaag aaguuauugu uaugaagcau      300

ccauaagcga uauggcaagc gauaguagau guccaacaca aggagaagca uaucuugaua      360

aacaaucaga uacccaauau guauguaaaa gaacauuagu agauagagga uggggaaaug      420

guuguggauu guucggaaaa ggauccuuag uaacaugcgc aaaauuuaca uguagcaaaa      480

aaaugacugg aaaaaguaua caaccugaaa aucuagaaua uagaauaaug uuaucuguac      540

auggaaguca acauucagga augauuguaa augacgaaaa uagagccaaa guagaaguga      600

caccaaauag cccaagagca gaagcaacau uaggagguuu ugguagcuua ggauuagacu      660

gugaaccacg aacagggcug gacuuuucag acuuauauua ucuaacuaug aauaauaaac      720

auuggcucgu acauaaagaa ugguuucacg auauaccacu accauggcau gcaggagcag      780

auacaggcac accacauugg aauaauaaag aagcauuagu agaauucaaa gacgcacacg      840

caaaaagaca aacaguagua guacuaggau cacaagaagg agcaguacau acagcuuuag      900

caggagcucu agaagcagaa auggauggag caaaagguag acuauucagc ggacauuuaa      960

aauguagauu aaaaauggau aaacuaaggu uaaaaggagu aaguuauagu uuauguacag     1020

cagcauuuac auuuacgaaa guaccagcag aaacauugca cggaacagug acaguugaag     1080

uacaauaugc gggaacagac ggaccaugca aaguaccagc acagauggcc guagauaugc     1140

aaacauuaac accaguaggu cgacuaauaa cugcaaaucc aguuauuaca gaaucuacag     1200

aaaauagcaa aaugaugcua gaguuagauc cuccauucgg agacaguuau auaguaauag     1260

gaguuggaga uaaaaaaaua acacaucauu ggcauagaag uggaaguaca auaggaaaag     1320

cauuugaagc aacaguaaga ggagcuaaaa gaauggcugu uuuaggagau acagcauggg     1380

acuuuggaag uugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  7121
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 2 opt

<400>  7121
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auacuuaguu augauacuau uaauagcacc ugcuuacagc auacgaugca      120

uugguguaag caaucgugac uuuguagagg gaaugagcgg uggaacaugg guagacguag      180

uauuagaaca cggaggaugu guaacaguaa uggcgcaaga uaaaccaacc guagauauag      240

aauuaguuac aacaacagua ucaaauaugg cagaaguaag aucauauugu uaugaagcaa      300

gcauaucaga uauggcaagu gacagucgau gcccaacaca aggagaagca uauuuagaua      360

aacaaagcga uacacaauac guauguaaaa gaacauuagu agauagggga uggggcaacg      420

guugcggauu auucggcaaa ggaagccuag uuacaugugc caaauuuaca uguaguaaaa      480

aaaugacugg aaaaucaaua caaccugaaa auuuagaaua uagaauuaug cuaaguguac      540

acggaagcca acacucagga augauuggau acgaaacaga ugaaaacagg gcuaaaguag      600

aagucacacc aaauucacca agagcagaag caacacuugg aggcuucggu ucgcuaggac      660

ucgauuguga accaagaaca ggacuagacu uuucugacuu auauuaucua acaaugaaua      720

auaaacauug gcuaguacau aaagaauggu uccacgauau accauugcca uggcacgcag      780

gugcagauac aggaacacca cauuggaaua acaaagaagc auuaguagaa uuuaaagacg      840

cccaugcuaa aagacaaaca guaguaguac uaggaaguca agagggugca guccauacag      900

cauuagcagg agcacuagaa gcagaaaugg auggggcuaa agguaaauua uuuaguggac      960

auuuaaaaug uagauuaaaa auggacaaau uaagauuaaa aggaguaagu uacagcuuau     1020

guacagcagc cuucacauuu acaaaaguac cagcagaaac auuacaugga acaguuacag     1080

uagaaguaca auaugcagga acagauggac cauguaaaau accaguccaa auggcaguag     1140

auaugcaaac auuaacacca guaggaagau uaauaacagc aaauccugua auaacagaaa     1200

guacagaaaa uagcaaaaug auguuagaac uagauccacc auuuggugac aguuauauag     1260

ugauaggagu uggagacaaa aaaauaacac aucauuggca ucgaucagga aguacaauug     1320

gaaaagcauu cgaagcaacu guaagaggag caaaaagaau ggcugucuua ggggacacag     1380

caugggauuu cggcaguuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  7122
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 2 opt

<400>  7122
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucuc       60

aaaaaguaau auauuuaguu augauacuau ugauagcucc cgcauauagu cuaagaugua      120

uaggaguaag caauagagau uuuguagaag gaaugagcgg uggaacaugg guagauguag      180

uauuagaaca uggaggaugu guaacaguua uggcacaaga uaaaccaaca guagauauag      240

aauuaguaac aacaacagua ucaaauaugg cagaaguaag aucauauugu uaugaagcaa      300

guauaagcga uauggcuuca gauaguagau gcccaaccca aggagaagca uaccuagaua      360

aacaaaguga uacacaauac guauguaaaa gaacguuagu agauagagga uggggaaaug      420

gaugcggauu auuuggaaaa ggaagucuag uaacaugugc uaaauuugca uguucaaaga      480

aaaugacagg aaaaucaauu caaccagaaa auuuagaaua uagaauaaug uuaagcguac      540

augguucaca acauagugga augaucguaa augauacagg acaugaaaca gaugaaaauc      600

gagcuaaagu agaaauaaca ccuaacaguc caagagcaga agcaacauua ggaggauuug      660

gaaguuuagg auuggauugc gaaccaagaa cagguuuaga uuucucagau uuauauuauc      720

uaacaaugaa uaacaaacau ugguugguac auaaagaaug guuucaugau auaccucuac      780

cuuggcaugc aggagcagac acgggaacac cacacuggaa uaauaaagaa gcauuaguag      840

aauuuaaaga ugcucaugca aaaagacaaa cuguaguagu auuaggaagu caagaaggag      900

caguucauac agccuuagca ggagcauuag aagcagaaau ggauggagcu aaaggaagau      960

uaucaagugg acauuugaaa ugcagauuga aaauggauaa auuaagauua aaaggaguau     1020

cauauagucu auguacagca gcauuuacau uuacaaaaau accagcggaa acacuacaug     1080

gcacaguuac ugucgaagua caauaugcag gaacagacgg uccauguaaa guaccagcac     1140

aaauggcagu cgauaugcaa acauuaacac cagugggaag auuaauaaca gcaaauccag     1200

uaauaacuga aucaacugaa aauucaaaaa ugaugcuaga auuagaucca cccuucggug     1260

auucguacau aguaauagga guuggggaaa agaaaauaac acaucauugg cauagaucag     1320

gaaguacaau uggaaaagca uucgaagcua cuguuagagg cgcuaaaaga auggcaguau     1380

ugggagauac agcaugggau uuuggaaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7123
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 2 opt

<400>  7123
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagca       60

aaaaaguaau auaccuagua augauauuac uaauagcacc agcauacagu auacgaugua      120

uaggaguaag uaacagagau uuuguugaag gaaugucagg aggaacaugg guugauguag      180

uauuagaaca cggcggaugu guaacaguaa uggcacaaga uaaaccaaca guugauauag      240

aauuaguaac cacaacaguu agcaauaugg cugaaguaag aucauauugu uaugaagcau      300

caauaucaga uauggcaagu gauucaagau guccaacaca aggugaagca uaucuagaca      360

aacaaucaga uacacaauac guauguaaaa gaacacuagu cgauagagga uggggaaaug      420

gaugcgguuu auucggaaaa gggucacuag uuacaugugc caaauuugca ugcagcaaaa      480

aaaugacugg aaaaucaauu caaccagaaa aucuugaaua uagaauaaug uuaucaguac      540

augguucaca acauagcgga augauuguua acgacaccgg acaugaaaca gaugaaaauc      600

gagcaaaagu agaaauaacu ccaaacucac caagagcuga agcaacauug ggaggauucg      660

gaucauuagg acuagacugu gaaccaagaa ccggauuaga uuuuucagac uuauauuauu      720

uaacaaugaa uaacaaacau ugguuaguac acaaagaaug guuucacgac auaccacuac      780

cauggcacgc aggagcugau acagguacac cacauuggaa uaacaaagaa gcucucguag      840

aauuuaaaga ugcacaugca aaacgacaaa cuguagucgu auuaggaucu caagaaggag      900

cuguacauac agcacuagca ggagcauuag aagcagaaau ggauggagcu aaaggaagau      960

uaagcucagg ccaucucaaa ugccgauuaa aaauggauaa auuaagauua aaaggaguau     1020

cauauucauu auguacagca gcauucacau uuacaaaaau uccagcugaa acauuacaug     1080

guacuguaac aguagaagua caauaugccg guacagaugg uccauguaag guaccugcac     1140

aaauggcagu agauaugcaa acauuaacac caguaggaag acuaauaaca gcaaauccug     1200

uaauaacaga aaguacagaa aauucaaaaa ugaugcuaga auuagaccca ccguucggcg     1260

auaguuauau uguaauagga guaggagaaa aaaaaauaac ucaccauugg cauagaucag     1320

gcucaacaau aggaaaagca uuugaagcaa caguaagagg agcuaaaaga auggcuguuc     1380

uaggagacac agcaugggau uuugguucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7124
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 2 opt

<400>  7124
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau cuauuuaguc augauauuau ugauagcccc agcauacucu auaagaugua      120

uaggaguaag uaauagagac uucgucgaag gaaugagugg aggaacaugg guagauguag      180

uucuagaaca uggaggaugu guaacaguaa uggcucaaga caaacccaca guagauauag      240

aauuaguaac aacaacagua agcaauaugg cagaaguacg aaguuauugc uaugaagcau      300

caauaucaga uauggcauca gauucaagau guccaaccca aggagaagcu uaucucgaua      360

aacaaucaga uacacaauau guauguaaaa gaacucuagu agauagggga uggggaaaug      420

guugcggucu auuuggaaaa ggaaguuuag ucacaugugc aaaauuugca ugcaguaaaa      480

aaaugacagg aaaaucuaua caaccagaga acuuagaaua uagaauaaug uugaguguac      540

acgguaguca acauaguggu augauaguca augauacagg acaugaaaca gaugaaaaua      600

gagcaaaggu agaaauaacg ccaaauucac caagagcaga agcaacauua ggaggauuug      660

gaagcuuagg acuagacugu gaaccacgaa cgggauuaga uuuuagugac cuauauuauu      720

uaacaaugaa uaacaaacau uggcugguuc auaaagaaug guuucacgau auaccacuac      780

cauggcaugc uggagcagau acagguacac cacacuggaa caauaaagaa gcauuaguag      840

aauuuaaaga cgcucaugca aaaagacaaa cgguaguagu cuuaggauca caagaaggag      900

caguacacac agcauuagcg ggagcacuag aagcagaaau ggauggugca aaaggaagau      960

uaaguagugg acaucuaaaa uguagauuaa aaauggauaa auuaagguua aaaggaguaa     1020

guuauucauu auguacggcg gcauuuacau uuacaaaaau accugcagaa acguuacaug     1080

gaacaguuac agucgaaguu caauaugcug gaacagaugg acccuguaaa guuccagcac     1140

aaauggcggu agauaugcaa acauuaacac caguuggaag auuaaucaca gcaaauccag     1200

uaauaacaga aaguacagaa aauaguaaaa ugaugcuuga auuagaucca ccauucggag     1260

auagcuauau aguaauugga guaggugaaa aaaaaauaac gcaucauugg uauagaucag     1320

gaucaacaau aggaaaagca uuugaagcaa cuguaagggg agcaaaacgu auggcaguau     1380

ugggagauac agccugggac uuuggaaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7125
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 2 opt

<400>  7125
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auaucuagua augauauuac uuauagcucc ugcauauagu auacgaugca      120

uagggguguc aaauagagau uuuguagaag gaaugagugg gggaacaugg guagauguag      180

uacuagaaca cggagguugu guaacaguca uggcacaaga uaaaccgacu guggauauag      240

aacuuguaac aacuacagua ucaaauaugg cagaaguaag aaguuauugc uacgaagcaa      300

guauaagcga cauggcaagc gauuccaggu guccaacaca aggagaagca uaccuagaua      360

aacaaagcga uacucaauau guauguaaaa gaacgcucgu agauagaggc uggggaaaug      420

gauguggauu auuugguaaa gggaguuuag uuacaugugc uaaauuugca ugcaguaaga      480

aaaugacagg aaaaaguaua caaccagaaa auuuagaaua ucgaauaaug uugucaguuc      540

auggaaguca acauucagga augauaguaa augauacagg acaugaaaca gaugaaaaua      600

gagcgaaagu ugaaauaaca ccaaauucac caagagcaga agcaacacua gggggauuug      660

ggucacuagg acuagauugu gaaccaagaa caggauuaga uuuuucagau cucuacuacc      720

uaacaaugaa caauaaacac ugguuaguac auaaagaaug guuucacgac aucccauuac      780

cauggcaugc aggagcagau acaggaacgc cacauuggaa uaacaaagaa gcauuaguag      840

aauuuaaaga ugcccaugcu aaaagacaaa caguuguagu acuugguuca caagaaggug      900

caguacacac agcauuagca ggagcauuag aagcagaaau ggauggagca aaaggaaaac      960

uaagcucugg acaucuaaaa ugcagacuaa aaauggauaa auuaagauua aaaggaguaa     1020

gcuauagucu augcacagca gcauucacuu ucacaaaaau accagcagaa acauuacacg     1080

gaacaguaac gguagaagua caauaugcag gaacugacgg accauguaaa gugccagcac     1140

aaauggcagu agauaugcag acauuaacac caguagguag auuaauaaca gcaaacccgg     1200

ugauuacuga aagcacugaa aauaguaaaa ugauguuaga acuagaucca ccuuuuggag     1260

auaguuauau aguuauagga guaggagaaa agaaaauaac acaccacugg cacagaucag     1320

gaucaacaau aggaaaagca uuugaagcaa caguaagagg agcaaaaaga auggcuguac     1380

uaggagauac agcaugggac uuugguaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7126
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 2 opt

<400>  7126
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auauuuagua augauacucc ucaucgcacc agcauacuca auaagaugua      120

uaggaguauc aaacagagac uuuguagaag gaauguccgg aggaacuugg guugauguug      180

uauuagaaca uggaggaugu guaaccguaa uggcacaaga caaaccaaca guagauauug      240

aacuaguaac aacaacagua ucaaauaugg cagaaguuag aaguuauugu uacgaagcau      300

caauaucaga uauggcauca gauucaaggu gcccaacaca aggagaagca uauuuagaca      360

aacaaucaga cacacaauau guaugcaaaa gaacacuggu cgauagagga uggggaaacg      420

gauguggauu guucggaaaa ggaucauuag uuaccugugc aaaauucgca ugcagcaaaa      480

aaaugacagg aaaaaguaua caaccagaaa auuuagaaua cagaauaaug uuaaguguuc      540

acggaucaca acauagugga augauuguua acgacacagg acacgaaaca gaugaaaaua      600

gagcaaaagu agaaauaacg ccaaauucgc caagagcaga agcaacauua gguggauuug      660

gaagcuuagg uuuagauugu gaaccaagaa caggacuuga uuuuagcgau uuauacuacc      720

uaacaaugaa uaauaaacau ugguuaguuc acaaagaaug guuucaugau auaccauuac      780

cauggcacgc aggagcagau acaggaacac cacacuggaa uaacaaagaa gcacuugucg      840

aauuuaaaga ugcgcacgca aaaagacaaa caguaguugu ucuaggauca caagaaggag      900

caguacauac agcucuagcu ggagcacuag aagcagaaau ggacggagca aaaggcagau      960

uauccagugg acauuuaaaa uguagacuaa aaauggacaa auuaagacuu aaaggaguua     1020

guuauucacu auguacagcc gcauucacau ucacaaaaau uccagcagaa acacuacaug     1080

gaacaguaac agucgaagua caauaugcag gaacagaugg accauguaaa guaccggcac     1140

aaauggcugu agacacucag acauuaacac caguaggaag guuaauuaca gcaaauccag     1200

uaauaacuga aucaacagaa aauagcaaaa ugauguuaga auuagaucca ccauuuggug     1260

auaguuauau uguuauagga guaggagaaa aaaaaauaac acaucauugg caucgaucag     1320

gaagcacaau aggaaaagca uuugaagcca cuguaagagg agcaaaaaga auggcaguac     1380

ucggugauac agcgugggac uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7127
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 2 opt

<400>  7127
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auaccuagua augauauuau uaauagcucc agcauauagu auaagaugca      120

uaggaguaag uaaucgagau uuuguagagg gaaugagcgg ggguacaugg guagacguag      180

uauuggaaca cggaggaugu guaacaguaa uggcacaaga caaaccaaca guagacauug      240

aacugguuac gaccacugua ucaaauaugg cagaaauaag aucauacugc uaugaagcga      300

guauaaguga uauggcauca gauucuagau guccaacaca aggagaagca uaucuagaca      360

aacaaaguga uacacaguau guauguaaaa gaacgcuagu agauagagga uggggaaaug      420

gauguggucu auuugggaaa gguagcuuag uaacaugugc aaaauuugca ugcaguaaaa      480

aaaugacagg aaaaucaauu caaccagaaa auuuagaaua uagaauaaug uuaucugugc      540

auggaucaca acauucagga augauaguga acgauacagg acacgaaacu gaugaaaaua      600

gagcaaaagu cgaaauaacc ccaaauucac ccagagccga agcaacauua ggaggauuug      660

gaucacuagg ccuugauugu gaaccaagaa caggauuaga cuuuucagac uuguauuauc      720

uaacaaugaa uaacaaacau ugguuaguac auaaagaaug guuucaugau auaccauuac      780

cuuggcaugc aggagcagau acggguacac cacauuggaa uaauaaagaa gcccucguag      840

aauuuaaaga ugcacaugcu aaaaggcaaa cuguuguagu acuagguagu caagaaggag      900

cuguacauac agcauuagca ggagcauuag aagcagaaau ggacggggca aaagguaggc      960

ucaguucagg acaccuaaaa ugcagauuaa aaauggauaa auuaagacua aaaggcguaa     1020

guuauaguuu auguacagca gcauuuacau ucacaaaaau accagcagaa acguuacaug     1080

gaacaguaac aguagaagua caauaugcag gaacagaugg accuugcaaa guuccagcac     1140

aaauggcagu agauaugcaa acacuaacac caguaggaag acuaauaacc gcaaauccag     1200

uaauaacaga gagcacagaa aauagcaaaa ugaugcuaga auuagaccca ccauucggag     1260

auuccuauau aguaauagga guaggugaaa aaaaaauuac acaucacugg caccgaucag     1320

gaucaacaau agguaaggcu uucgaagcaa caguaagagg agcuaaacgu auggcaguau     1380

uaggagauac ugcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7128
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 2 opt

<400>  7128
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auacuuaguu augauuuugu uaauagcacc agcauauuca auaagaugca      120

uuggaguauc aaauagagau uucguagaag gaaugagcgg aggcacaugg guagauguag      180

uauuagaaca ugguggaugu guuacuguaa uggcacaaga uaaaccaacu guagacauag      240

aacuaguaac aacaacagua ucaaauaugg cagaaguaag aaguuauugu uaugaagcaa      300

gcauauccga uauggccuca gacaguagau guccaacaca aggagaagcu uacuuagaua      360

aacaaucaga uacacaauau guauguaaaa gaacacuagu agacagagga uggggaaaug      420

gaugcgguuu auucggaaaa ggaagucuag uaacaugcgc aaaauuugca uguaguaaaa      480

aaaugacagg aaaaaguaua caaccagaaa auuuagaaua cagaauaaug cuaagugucc      540

auggaaguca acauagugga augauuguaa augacacagg acaugaaaca gaugaaaaua      600

gagcgaaagu agaaauaaca ccaaacucac caagagcaga agcaacauua ggaggauuug      660

gaucauuagg ucuugauugu gaaccaagaa cuggacuuga uuuuucagau uuauauuacc      720

uaacaaugaa uaauaaacac uggcugguac auaaagaaug guuccaugac auaccucuac      780

ccuggcacgc aggagcagau acagguaccc cacauuggaa uaauaaagaa gcauuaguag      840

aguucaaaga ugcacaugca aaaagacaaa caguaguugu auuaggauca caagaaggag      900

caguacauac agcacuagca ggagcauuag aagcagaaau ggacggcgca aaaggcagac      960

uuagcagugg acaccuaaaa ugccgcuuaa aaauggauaa acuaagauua aaaggaguaa     1020

guuauucauu gugcacagca gcauucacau ucacaaaaau acccgcagaa acacuacacg     1080

guacaguuac aguugaagua caauaugcag gaacagaugg accauguaaa guuccagcac     1140

aaauggcagu agauaugcaa acauuaacac caguaggucg auuaauaaca gcaaacccag     1200

uaauuacaga aucaacagaa aauaguaaaa ugauguuaga acucgacccc ccauucggag     1260

acucauacau aguuauagga guaggugaaa aaaaaauuac acaucacugg cacagaucag     1320

gaucaacaau aggaaaagcu uuugaagcaa caguuagagg agcacgaaga auggcuguau     1380

uaggagauac cgcgugggac uuuggaaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7129
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 2 opt

<400>  7129
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucuc       60

aaaaaguuau auauuuagua augauuuuau uaauagcacc agcauauuca auuagaugua      120

uaggaguauc aaauagagau uuuguagagg gaaugucugg aggaacaugg guagauguag      180

uucuagaaca uggaggaugu guaaccguaa uggcgcaaga uaaaccaacu gucgauauag      240

aauuaguaac aacaaccguu ucaaauaugg cagaaguaag aucauauugu uaugaagcau      300

caauaaguga uaugguaagc gacucaagau guccaacaca aggagaagca uaucuggaua      360

aacaaucgga cacgcaauau guauguaaaa ggacucuagu ugauagagga uggggaaaug      420

gaugcgguuu auuugguaaa ggaaguuuag uaacaugcgc aaaauuugca uguucaaaaa      480

aaaugacagg aaaauccaua caaccugaaa auuuagaaua cagaauaaug uuaucaguac      540

auggaaguca acauucagga augauaguaa acgacacagg acaugaaaca gaugaaaaca      600

gagcuaaagu ggaaauaaca ccgaauagcc caagagcaga ggcaacauua ggaggauuug      660

gaucacuggg auuagauugu gaaccaagaa caggcuuaga uuuuagcgac uuauacuauu      720

uaacaaugaa uaauaaacau uggcuagugc auaaagaaug guuccacgau auaccauuac      780

cguggcaugc aggagcagau acaggaacac cacauuggaa uaacaaagaa gcuuuaguag      840

aauuuaaaga cgcccaugca aaaagacaaa cgguaguagu auugggauca caagaaggug      900

caguacauac agcacuagca ggagcauuag aagcagaaau ggauggcgca aaaggaagac      960

uaucgucagg acacuuaaaa uguagauuaa agauggacaa acuuagauua aaaggcguaa     1020

guuauucauu gugcacagca gcauuuacau uuacaaaaau accugcagaa acauuacaug     1080

gaacaguaac uguagaagua caauaugcag gaacagacgg uccaugcaaa guaccagcac     1140

aaauggcagu agauaugcaa acacuaacac caguaggaag acuaauaacc gcaaauccag     1200

ucauaacaga aucaacagaa aauagcaaaa ugaugcuaga auuagauccu ccauucggag     1260

auagcuauau agucauaggg guuggggaaa aaaaaauuac ucaccacugg cauagaagug     1320

gaagcaccau aggaaaagca uuugaagcaa caguaagagg agccaaaaga auggcagugu     1380

uaggagauac ggcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7130
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 2 opt

<400>  7130
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaaguaau auaccuagua augauacuau uaauagcacc ugcauauagu auaagaugua      120

uaggaguauc aaauagagau uucguagaag gaaugucagg aggcacaugg guagacguag      180

uuuuagaaca uggaggaugu guaacuguaa uggcacaaga uaaaccuacu guagauauag      240

aauuaguaac aacaacagua ucaaacaugg cagaaguaag aaguuauugu uaugaagcaa      300

gcauaucuga uauggcauca gacucaagau gcccuacaca aggagaagcg uacuuagaca      360

aacaaagcga uacacaauau guauguaaaa gaacacucgu agauagagga ugggggaacg      420

gaugugguuu auucgggaaa ggaucccuag uaacaugugc aaaauuugca uguaguaaaa      480

aaaugacagg gaaaucaaua caaccugaaa accuagaaua uagaauaaug uuaagcguac      540

auggaucaca acauucagga augauuguaa acgacacagg acaugaaaca gaugaaaaua      600

gagcaaaagu agaaauuaca ccaaauucac caagggcuga agcaacauua ggagguuuug      660

gaaguuuagg auuagauugu gaaccaagaa caggacuaga uuucagugau uuguacuacu      720

uaacaaugaa uaauaaacau uggcuuguuc auaaagaaug guuccaugau auaccacuac      780

cauggcacgc aggagcagau acuggaacac cucacuggaa caauaaagaa gcacuaguug      840

aauucaaaga cgcccaugca aaacgacaaa cugucguagu auuaggauca caagaaggag      900

caguacauac agcauuagca ggagcauuag aagccgaaau ggauggagcu aaaggaagau      960

uaucaucagg acaucuaaag uguaggcuaa aaauggacaa acuaagauua aaaggaguga     1020

guuacaguuu auguacagcc gcauuuacau uuacaaaaau cccagcagaa acacugcaug     1080

gaacuguaac aguugaagua caauaugcag gaacagaugg gccaugcaaa guaccagcac     1140

aaauggcagu agauaugcaa acacuaacac caguaggaag guuaauaaca gcuaauccag     1200

uaauaacaga aaguauagaa aauaguaaaa ugauguuaga acuagaccca ccuuucggug     1260

auucuuauau aguaauagga guaggugaaa aaaaaauaac acaucauugg cauagaucug     1320

gaaguaccau aggaaaagca uuugaagcua caguaagagg agcuaaaaga auggcaguau     1380

uaggagauac agcaugggau uucgguaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7131
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 2 opt

<400>  7131
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auaccuagua augauauuac uaauagcacc agcuuacuca auaagaugua      120

uagggguauc aaauagagau uuugucgaag gaaugucugg aggaacaugg guagaugucg      180

uacuugaaca cggaggaugu guaacaguaa uggcacaaga uaaaccaaca guagauauag      240

aauuaguuac aacaacagua ucaaacaugg cugaaguaag aucauacugu uacgaagccu      300

caauuucaga uauggcaagu gauucuaggu guccaacgca aggagaagcc uauuuagaua      360

aacaauccga uacacaauau guuuguaaaa gaacuuuagu agauagagga ugggguaacg      420

gauguggauu auucggaaaa gggucucuag uaacaugugc aaaauuugca uguaguaaaa      480

aaaugacagg aaaaucaaua caaccugaaa aucuagaaua cagaauaaug uuaaguguac      540

auggaagcca acauucagga augauaguaa augauacagg acaugaaaca gaugaaaacc      600

gugccaaagu agaaauaaca ccaaacucac cacgagcaga agcaacuuua ggaggauuug      660

ggucacuagg acuagacugu gaaccaagaa cuggacuuga cuuuucugau cuauauuauu      720

uaaccaugaa uaacaaacac uggcuuguac acaaagaaug guuccaugau aucccauuac      780

cauggcacgc aggagcagau acaggaacac cccauuggaa uaauaaagaa gcauuaguag      840

aauuuaaaga ugcacacgca aaacgacaaa cagucguugu auuaggauca caagaaggag      900

caguacauac agcacuagca ggagcacuag aagcagaaau ggauggagca aaaggaagau      960

uaucaagcgg acaccuaaaa uguagacuaa aaauggauaa auuaagauua aaaggaguaa     1020

guuauaguuu auguacagca gcauucacau ucacaaaaau accagcagaa acacuacaug     1080

gaacagucac aguagaagua caauaugcug gaacagaugg accuuguaaa guaccagcac     1140

aaauggcagu agauaugcaa acauuaacuc caguaggaag auuaauuaca gcaaauccug     1200

uaauaacaga aucaaccgaa aacucaaaaa ugauguuaga auuagaccca ccauuuggag     1260

acucauauau agucauagga auaggcgaaa aaaaaauaac acaucacugg cacagaagug     1320

gcucaacaau aggaaaagca uuugaagcga caguaagggg agcaaaaaga auggccguau     1380

uaggagacac cgcaugggac uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7132
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 2 opt

<400>  7132
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguaau auaucugguu augauuuugu uaauagcauu agcauacuca auaagaugca      120

uaggaguaag uaauagggau uuuguagaag gaaugagugg agguacaugg guagauguag      180

uauuagaaca ugguggaugc guaacaguaa uggcacaaga uaaaccuaca guagacauag      240

aauuaguaac caccacaguu ucaaacaugg cagaagucag aucauacugu uacgaagcaa      300

gcauauccga uauggcauca gacucuagau guccaaccca aggugaagca uauuuagaca      360

aacaaucaga cacacaauau guauguaaac gaacuuuagu agauagagga uggggaaaug      420

guugcggauu auuuggaaaa ggaucauuag uaacaugugc aaaauucgca ugcaguaaaa      480

aaaugacugg aaaaucaaua caacccgaaa auuuagaaua uagaauaaug uuaaguguuc      540

auggcaguca acauaguggu augauaguaa augacacagg acaugaaaca gaugaaaaca      600

gagcaaaagu ugaaauaaca ccaaacucuc caagagcaga agcaacauua ggagguuuug      660

guaguuuagg acuagauugu gaaccaagaa cuggauuaga uuuuucagau cuauauuauu      720

ugacaaugaa uaauaaacau ugguuaguuc acaaagaaug guuccaugau auaccacuac      780

cauggcaugc aggagcagau acaggaacac cacauuggaa uaauaaagaa gcauuagugg      840

aauuuaaaga ugcacaugca aaacgacaaa caguaguugu acuaggauca caagaaggag      900

caguacacac ggcauuagca ggagcauuag aagcagaaau ggauggggca aaaggaaggu      960

uaagcuccgg gcauuuaaaa ugccgauuaa aaauggacaa auuaagauua aaaggugugu     1020

cuuauagucu auguacagca gcauuuacau uuacaaaaau accggcagaa acacuucaug     1080

guacaguaac aguagaagua caauaugcag gaacagaugg cccauguaaa gugccagcac     1140

aaauggcagu agacaugcaa acacuaacac cagucggaag auuaauaacu gcaaauccag     1200

uaauaacaga aucaacagaa aacaguaaaa ugauguuaga auuagacccu ccguucggag     1260

acuccuauau aguaauagga guaggugaaa aaaaaauaac acaucauugg cauagaucag     1320

gaucaacaau aggaaaagcc uuugaagcaa caguaagagg agcaaaaaga auggcaguac     1380

uaggagacac agcaugggac uuuggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7133
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 2 opt

<400>  7133
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau uuacuuaguu augauacuac uuauagcacc agcauauuca auaagaugua      120

uaggaguauc aaauagggac uuuguagaag gaaugagugg ugguacuugg guagacguag      180

uauuagaaca cggaggaugu guaacuguaa uggcgcaaga uaaaccaaca guagacauag      240

aauuaguuac aaccaccgua aguaauaugg cagaaguaag aaguuauugc uacgaagcaa      300

guauaucaga cauggcaagu gauucaagau guccaacuca aggugaagca uauuuagaua      360

aacaaucaga uacacaauau guauguaaaa gaacucuagu agacagagga ugggguaaug      420

gaugcggcuu auucggaaaa ggauccuuag uuacaugugc uaaauuugcu uguaguaaga      480

aaaugacagg aaaaaguauu caaccagaaa acuuagaaua cagaauaaug uugagcguac      540

augguaguca gcauucagga augauaguca augauacagg acaugaaaca gaugaaaaua      600

gagcaaaagu agaaauaaca ccaaauaguc caagagcaga agcgacauua ggaggauuug      660

gaagccuagg acuagacugu gaaccaagaa ccgguuuaga uuucuccgau uuauacuauu      720

uaacaaugaa caacaaacac uggcuagucc auaaagaaug guuucaugau auuccacuac      780

cauggcaugc aggagcagac acggguacac cacacuggaa uaauaaagaa gcauuaguag      840

aauuuaaaga ugcacaugca aagagacaga caguaguugu auuaggaaca caagaaggug      900

caguacacac agcacuagcu ggugcacuag aagcagaaau ggauggagca aaaggccgac      960

uaaguagugg acaccuuaaa uguagauuaa aaauggauaa auuaagauua aaaggaguuu     1020

cuuauaguuu auguacagca gcauucacau uuacaaaaau accagcugaa acauuacacg     1080

gaacaguaac aguugaaguu caauaugcag gaacagaugg accauguaaa guaccugcgc     1140

aaauggcugu agauaugcaa acguuaacac caguaggaag auuaauaacu gcaaacccag     1200

uuauaacaga aucuacagaa aauaguaaaa ugauguuaga auuagacccu ccauuuggcg     1260

auaguuauau aguaauagga guaggagaaa agaaaauaac acaccacugg cauagaucag     1320

gaaguacaau aggaaaagca uuugaagcua caguacgagg cgcuaaaagg auggcuguau     1380

uaggugauac agcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7134
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 2 opt

<400>  7134
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguuau auauuuaguu augauacuau uaauagcacc agcuuacagu auaagaugua      120

uaggaguaag uaauagagau uuuguagagg guaugucagg aggaacaugg guagacguag      180

uauuagaaca uggaggaugu guaacaguaa uggcacaaga uaaaccaaca guagauauag      240

aacuaguaac gacaacagua ucaaauaugg cugaaguuag gucauauugu uaugaagcaa      300

guauuaguga cauggcauca gauagcagau guccaacaca aggagaagca uaucuagaca      360

aacaaaguga uacacaauau guaugcaaaa gaaccuuagu agauagagga uggggaaacg      420

gcuguggucu auuuggaaaa ggaucauuag uaacaugcgc aaaauuugca ugcaguaaaa      480

aaaugacagg aaaaucaaua caacccgaaa aucuagaaua uagaauaaug uuaaguguac      540

auggaucaca acacucgggc augauaguaa augauacagg acaugaaaca gaugaaaaua      600

gagcgaaagu agaaauaaca ccaaauaguc cuagagccga agcaacguua ggaggauuug      660

gcucacuugg acuggauugu gaaccaagaa ccggacuaga uuuuucagau uuauauuauu      720

uaacaaugaa caauaaacau ugguuaguac acaaagaaug guuucaugau auccccuuac      780

cauggcacgc aggugcagau acaggaacac cacauuggaa uaauaaagag gcauuaguag      840

aauuuaaaga cgcacaugca aaaagacaaa caguaguagu auuaggaagu caagaaggag      900

caguacauac agcauuagcu ggagcccuag aagcggaaau ggacggagca aaaggaaggu      960

uaucaagugg acauuuaaaa uguagacuaa aaauggauaa auuaagacua aaaggaguau     1020

cauacagcuu guguacagca gcauuuacau uuacaaaaau accagcagaa acauuacacg     1080

guacaguuac aguagaagua caauaugcag gaacagacgg accuugcaaa guauuagcac     1140

aaauggccgu agauaugcaa acacuaacac caguaggaag auuaauaaca gcaaauccag     1200

uaauaacaga aagcacagaa aacaguaaaa ugauguuaga acucgaucca cccuucggag     1260

auuccuacau agucauaggu gugggagaaa aaaaaauuac acaucauugg cauagaagcg     1320

gaagcacaau aggcaaagca uuugaagcaa cagucagagg agcaaagcga auggcaguuc     1380

uaggagacac agccugggau uuuggaaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7135
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 2 opt

<400>  7135
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau auauuuagua augauauuac uaauagcacc agcauauagu auacgcugua      120

uuggaguauc gaaucgagac uuuguagaag gaaugagcgg aggaacaugg guagauguag      180

uacuagaaca cggaggaugu guuaccguua uggcacagga uaaaccaaca guagauauag      240

aauuaguaac aacaacaguu aguaauaugg cagaaguaag aagcuauugu uacgaagcau      300

caauaagcga cauggcaagc gauaguagau gcccaacaca aggagaagca uauuuagaua      360

aacaaaguga uacucaauau guaugcaaac gaacauuagu agacagagga uggggaaaug      420

gauguggauu auuuggaaaa ggaagucuag uuacaugcgc aaaauuugcu uguagcaaga      480

aaaugacagg aaaaucaaua caaccagaaa acuuggaaua uagaauaaug uuaucuguac      540

auggaucaca acauucagga augauaguaa acgauacugg acacgaaaca gaugaaaaua      600

gagcaaaagu ugaaauuaca cccaauagcc caagagcaga agcaacauua gggggauucg      660

gaucauuagg auuagacugu gaaccaagaa caggauuaga cuucucagau uuguauuauu      720

uaacaaugaa uaauaaacac uggcuaguac acaaagaaug guuucaugau auaccucucc      780

cauggcacgc aggagcugau acaggaacac cacacuggaa uaacaaagaa gcauuaguag      840

aauuuaaaga agcacaugca aaaagacaaa cuguuguagu acuaggaagu caagaaggag      900

caguacacac ugcauuagca ggagcuuuag aagcggaaau ggauggagca aaaggaagau      960

uaucaagugg acaucuaaaa uguagacuaa aaauggauaa acuaagauua aaaggcguaa     1020

guuauagucu auguacggcu gcguucacau ucacaaaaau accagcagaa acauuacacg     1080

gaacaguaac aguagaagua caauaugcag gcacagaugg acccugcaaa guaccagcac     1140

aaauggcagu agauaugcaa acauuaacac caguaggaag acuaauaaca gcaaauccug     1200

uaauaacaga aaguaccgaa aauaguaaaa ugauguuaga acuagaucca ccauuuggag     1260

auaguuauau aguaaucgga guuggagaaa agaaaauaac ucaucacugg cacagaagcg     1320

guaguaccau agguaaagca uuugaagcua cagugcgagg agcaaaaaga auggcaguuu     1380

uaggagauac agcaugggau uuuggaaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7136
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 2 opt

<400>  7136
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auacuuaguu augauauuac ucaucgcucc ggcauauagu auaagaugua      120

uuggaguauc aaauagagau uuuguagaag gaaugucagg aggaacaugg guagaugugg      180

uauuagaaca uggcggaugc guaacaguaa uggcacaaga uaaaccaaca guagacauag      240

aauuagucac cacaacaguc agcaauaugg cagaaguaag aagcuauugc uaugaagcau      300

caauaaguga uauggcaagu gauagcagau guccaacaca aggagaagcu uaucuagaua      360

aacaaaguga uacacaauac guauguaaaa gaacauuagu ugacagagga uggggaaacg      420

gcuguggacu auuuggaaaa ggaaguuuag uaacaugcgc aaaauucgca uguagcaaaa      480

aaaugacagg aaaaaguauu caaccagaaa accuagaaua uagaauaaug uuaagcguac      540

auggaaguca acauucagga augauaguua acgauacagg acaugaaaca gaugaaaaua      600

gagcaaaagu agaaauaacc ccaaauaguc cuagagcaga agcagcauua ggaggauuug      660

gaucacuagg auuagauugc gagccaagaa caggguuaga uuuuucagau uuauauuauc      720

uaacaaugaa uaacaaacau ugguuaguac acaaagaaug guuccacgau auaccauuac      780

cauggcacgc aggcgcggac acaggaacac cccauuggaa caauaaagaa gcauuaguag      840

aauuuaaaga ugcgcaugca aaacgacaaa cuguaguagu acuuggaagu caagaaggag      900

cugugcauac agcucuggca ggagcuuuag aagcagaaau ggauggagca aaaggaagau      960

uaucaagugg ucacuuaaaa uguagauuaa aaauggauaa acuaagguua aaagguguga     1020

gcuacaguuu augcacagcu gcauuuacau uuaccaaaau accugcagaa acacuacacg     1080

gcacaguuac uguagaaguc caauaugcag gaacagacgg accauguaaa gugccagcac     1140

aaauggcagu agacaugcaa acauuaacac caguggggag acuaauaaca gcaaauccag     1200

uaauaacaga aaguacagaa aauaguaaaa ugauguuaga auuagaucca ccauucggag     1260

auucauauau agucauaggu gugggugaaa aaaaaauuac acaucauugg cauagaucag     1320

gaucaacgau cgguaaagca uuugaagcaa caguacgugg ggcgaaacga auggcaguau     1380

uaggagauac ggcaugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7137
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 2 opt

<400>  7137
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auaucuagua augauauuau uaauagcacc ggcauauagc auaagaugua      120

ucggaguaag uaauagagac uuuguagaag gaaugagugg aggaacaugg guugauguag      180

uacuagaaca uggaggaugc gugacaguaa uggcucaaga uaaaccaaca guagauauag      240

aacuaguaag cacaacagua aguaauaugg cagaaguuag aagcuauugu uacgaagcaa      300

caauaaguga uauugcaagu gacaguagau guccaacaca aggagaagca uaucuagaua      360

aacaaaguga uacacaauau guauguaaaa gaacgcuugu agauagagga uggggaaaug      420

gcuguggacu auuuggaaaa ggaagccuag uaacaugugc aaaauucgca ugcaguaaaa      480

aaaugacagg aaaaaguaua cagccagaaa auuuagaaua cagaauaaug cuaaguguac      540

auggaucaca acacagugga augauaguaa acgauacagg acaugaaaca gaugaaaaca      600

gagcaaaagu agaaauaaca ccgaauagcc cgagggcuga agcaacauua ggaggauuug      660

gaagcuuagg auuagauugu gaaccaagaa caggauuaga uuuuagugac uuauacuauu      720

uaacaaugaa uaauaaacac ugguuaguac auaaagaaug guuccaugac auaccccuac      780

cuuggcaugc aggugcagau acaggaacac cacauuggaa uaauaaagaa gcauuaguag      840

aauuuaaaga ugcucaugcu aaaagacaaa cagcaguugu acuaggauca caagaaggag      900

caguacauac agcacucgca ggggcauuag aagcagaaau ggacggagca aaaggcagau      960

uaucaagcgg gcauuuaaaa uguagacuaa aaauggauaa auuaagacua aaaggugugu     1020

cauauucacu auguacagcu gcauucacau uuacaaaaau accagcagaa acauuacacg     1080

gaacggucac agucgaaguc caauacgcag gaacagaugg accauguaaa guacccgcac     1140

agauggcagu agauaugcaa acacuuacac caguagguag auuaauaaca gcaaauccag     1200

uaauaacaga aaguacagaa aacaguaaaa ugaugcuaga auuagaucca ccauuuggag     1260

auucauauau aguaauugga gugggcgaaa aaaaaauuac acaucacugg caucgaagug     1320

gaucaacaau aggaaaagca uuugaagcaa ccguaagggg agcuaaaagg auggcagucu     1380

uaggagacac agcuugggau uucgguucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7138
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 2 opt

<400>  7138
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaaguaau auaucuagug augauauuau uaauagcacc agcauauagc auaagaugua      120

uaggaguguc aaacagagau uucguagaag gaaugucagg aggaacaugg guagaugugg      180

uacuagaaca uggagguugc guuaccguaa uggcacaaga uaaaccaaca guagacauag      240

aauuaguaac aaccaccgua aguaauaugg cagaaguuag aaguuauugu uaugaagcaa      300

gcauaagcga cauggcaagc gacagcagau guccaacaca aggagaagca uauuuagaua      360

aacaaagcga cacacaauau guaugcaaaa gaacuuuagu agauaggggu uggggaaaug      420

gcuguggacu auuuggaaaa gguaguuuag uaacaugugc aaaauuugca uguucaaaga      480

aaaugacugg aaaaucaaua caaccagaaa auuuagaaua ucguauaaug cuaaguguac      540

auggaucaca acacagugga augauaguaa augauacagg acaugaaaca gaugaaaaua      600

gagcaaaagu agaaauaaca ccaaacucac ccagagcuga agcaacguua ggaggauuug      660

gaucauuagg auuagauugu gaaccaagga caggauuaga uuuuagugau cuauauuauc      720

uaacaaugaa uaauaaacac ugguuaguuc auaaagaaug guuucaugau auaccauuac      780

cauggcaugc aggagcagac acaggaacac cacauuggaa caauaaagaa gcgcuagugg      840

aauuuaaaga ugcacacgca aaaagacaaa cuguaguugu acuaggaagu caagaagggg      900

caguacauac ggcucuagcu ggagcacugg aagcagaaau ggacggggcu aaagguagau      960

uaucgagcgg acauuuaaaa uguagguuga aaauggacaa acuuagauua aaagguguau     1020

cauauaguuu auguacagca gcguuuacgu uuaguaaaau accugcagaa acauuacacg     1080

gaacaguaac agaagaagua caauacgcag gaacggaugg ucccugcaaa guuccagcac     1140

aaauggcugu ggacaugcaa acauuaacac cuguaggaag auuaauaaca gcaaacccag     1200

uaauaacaga aagcacagaa aauaguaaaa ugauguuaga auuagaucca ccauuuggag     1260

auaguuauau cguaauagga guaggagaaa agaaaaucac ucaucacugg cauagaagcg     1320

guaguacaau aggaaaagca uuugaagcaa caguaagagg agcaaaaaga auggcaguac     1380

uaggagauac agcgugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7139
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 2 opt

<400>  7139
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau uuauuuaguu augauauuac uaauagcacc agcauauuca auaagaugua      120

uaggagugag uaaucgagau uuuguagaag gcaugagugg aggaacuugg guagauguag      180

uuuuagaaca cggcggaugc guaacaguaa uggcacaaga uaaaccgacg guagacauag      240

aauuaguaac aacaacaguu aguaacaugg cagaaguaag aucauauugu uaugaagcaa      300

guauaaguga uauggcuagu gauucaagau guccaacaca aggcgaagca uacuuagaua      360

aacaaagcga uacacaauau guauguaaga gaacauuagu agauagaggu uggggaaaug      420

gauguggauu auuuggaaaa ggaucacuag uaaccugcgc aaaauuugcu uguucaaaaa      480

aaaugacagg aaaaucaauc caaccagaaa aucuagaaua uagaauaaug uuaucaguac      540

augggagcca acacucggga augauaguaa augauacugg ccacgaaaca gacgaaaauc      600

gagcaaaagu agaaauaaca ccaaauucac caagagcaga agcaacacua ggaggauuug      660

gaucauuagg auuagacugu gaaccaagaa cuggacuaga uuuuucagac cuauacuauc      720

uaacaaugaa uaauaaacac ugguuaguac auaaagaaug guuucaugac auaccacugc      780

cauggcaugc aggcgcagau acgggaacgc cacauuggaa caauaaagaa gcauuaguug      840

aauuuaaaga ugcacaugcg aaaagacaaa cuguaguagu auuaggaagc caagaaggag      900

caguucacac cgcauuagcu ggagcguuag aagcagaaau ggacggagca aaagggagau      960

uaucaagcgg ucaucuaaaa ugcagacuaa aaauggauaa auuaagauua aaaggaguaa     1020

gcuauucgcu auguacagca gcauuuacau uuacaaaaau accagcagaa acauuacaug     1080

gaacaguaac aguagaauua caauaugcug gcacagaugg accauguaaa guaccagcac     1140

aaauggcagu agauaugcaa acucuuacac caguaggacg auuaauuaca gcaaauccag     1200

uaauaacuga aucuacugaa aacaguaaaa ugauguuaga auuagaucca ccauuuggag     1260

auaguuauau aguaauagga guaggagaaa aaaaaauaac acaucauugg cauagaucag     1320

guaguacaau aggaaaagca uuugaagcaa caguaagagg agcaaaaaga auggcaguuu     1380

uaggugauac agccugggau uuugguucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7140
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 2 opt

<400>  7140
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaagugau cuauuuagua augauucuac uaauagcacc agcauauuca auaagaugua      120

uaggcauauc uaacagggau uuuguugaag gaaugagugg aggaacuugg guagacguag      180

uauuggaaca uggaggaugu guaacaguaa uggcacaaga uaaaccuaca guagacauag      240

aacugguaac aacaacagua aguaauaugg cugaaguaag gucauacugu uacgaagcaa      300

guauaagcga uauggcaagu gauucaagau gcccaacaca aggagaagca uaucucgaua      360

aacaaagcga uacacaauau guauguaaaa gaacauuagu agauagagga uggggaaacg      420

guugcggauu auuugggaaa ggaucuuuag uaacaugugc aaaauucgca uguaguaaaa      480

aaaugacagg aaaauccaua caaccagaaa acuuagaaua uagaauaaug uuaaguguac      540

acgguaguca acauucagga augauuguaa augauacagg acacgaaacc gacgaaaaua      600

gagcaaaagu agaaauaaca ccaaacucac caagagcaga agcaacacua ggaggauuug      660

gaauacuugg auuagauugu gaaccacgaa cuggacuaga uuuuucagac cuauauuauc      720

uaacgaugaa uaauaaacau uggcuaguac auaaagaaug guuucaugau auacccuuac      780

cauggcaugc aggcgcagau acgggaacgc cacauuggaa caacaaagaa gcauuaguag      840

aauuuaaaga ugcacaugca aaacgacaaa caguaguagu auuaggguca caagaaggag      900

caguacauac ugcacuugca ggagcuuuag aagcagaaau ggacggugca aaaggaagau      960

uaucaucagg acacuuaaaa uguagauuaa aaauggacaa auuacgauua aaaggaguau     1020

cauauagcuu augcaccgca gcauuuacau uuacuaaaau accugcugaa acauuacaug     1080

gaaccguaac aguagaagua caauaugcag gaacagaugg accauguaaa guaccagcac     1140

aaauggcagu agauaugcaa acacuaacac caguaggaag acuuaucaca gcaaauccag     1200

uaauaacaga aagcacggag aauucaaaaa ugauguuaga acuagaucca ccauuuggag     1260

acucauacau aguaauagga guaggagaaa aaaaaauaac acaucauugg cacagaucag     1320

gaaguacuau aggaaaagca uuugaagcaa cgguaagagg agcaaagaga auggcaguau     1380

uaggagauac agcaugggau uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7141
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 2 opt

<400>  7141
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auauuuagua augauauuau uaauagcacc agcauauagu auaagaugua      120

uaggaguaag uaauagagau uucguagaag gaaugagugg gggaacaugg guagauguag      180

uauuagaaca uggagggugu guaacaguaa uggcacaaga caaaccaacc guggauauug      240

aauuaguaac uacuacaguc aguaauaugg cagaagucag aucauauugu uacgaagcua      300

guauaucaga cauggcgagu gauagcagau guccaacaca aggagaagca uauuuagaua      360

aacaaaguga uacucaauau guauguaaaa gaacauuagu ugauagaggg uggggaaaug      420

gauguggauu auuuggaaaa ggaagcuuag uaacaugugc aaaauucgca uguaguaaaa      480

aaaugacagg aaaaaguaua caaccagaaa acuuagaaua uagaauaaug cugaguguac      540

auggaucaca acauucagga augauaguaa augauacagg acaugagaca gaugaaaaca      600

gagcaaaggu agaaauaacu ccaaacaguc cacgagcaga agcaacacua gguggauuug      660

gaucacuagg acuagauugu gaaccuagaa caggauuaga uuuuucagau uuauauuauc      720

uuacaaugaa caacaaacau uggcuaguac auaaagaaug guuccacgau auaccacuac      780

cauggcaugc uggagccgau accggcacuc cacauuggaa caauaaagaa gcauuaguag      840

aauucaaaga ugcacacgcu aaaagacaga cagugguagu auuaggaagu caagaaggag      900

caguacacac agcacuagca ggagccuuag aagcagaaau ggauggggua aaagguaggu      960

uaagcagugg acacuuaaaa uguagauuaa aaauggauaa acuaagacuu aaagguguaa     1020

guuacucauu auguacagca gcuuuuacgu uuacuaaaau cccugcagaa acacuacaug     1080

gaacaguaac cguggaagua caauacgcag gaacagaugg accauguaaa guaccagcac     1140

aaauggcugu agauaugcaa acauuaaccc caguaggaag auugauaaca gcaaauccag     1200

uaauaacaga aaguacagaa aauaguaaaa ugauguuaga auuagauccu cccuuuggug     1260

auagcuauau aguuauaggu guaggagaaa aaaaaauaac acaucauugg cacagaucag     1320

gcagcacaau agguaaagcu uuugaagcaa caguaagagg agcaaaacga auggcagugu     1380

ugggagauac agccugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7142
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 2 opt

<400>  7142
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau uuauuuagua augauacuau uaauagcacc agcauauagu auaagaugua      120

uagggguauc caacagagau uucguugaag gaaugagcgg aggaacaugg guagauguag      180

uuuuagaaca ugguggaugu guaacaguaa uggcacaaga uaaaccuaca guagauauag      240

aauuaguaac aacaacagua ucaaacaugg cagaaguaag aaguuauugc uaugaagcaa      300

gcauuaguga uauggcaagu gauagucgau guccaacaca aggagaagca uaucuagaca      360

aacaaaguga cacacaauac guauguaaaa gaacauuagu agauagagga ugggguaaug      420

gcugcggacu auucgggaaa ggauccuuag uaacuugugc aaaauuugcc uguaguaaaa      480

aaaugacagg aaaaucaaua caacccgaaa aucuagaaua ccgaauaaug cuaaguguac      540

acggaaguca acauucagga augaucguga augacacagg gcaugaaacu gaugaaaaca      600

gagcaaaagu agaaauuaca ccaaauucac caagggcuga agcaacauua ggaggauucg      660

gaaguuuagg acuggauugu gaacccagaa cuggauuaga cuuuucagau uuauacuauu      720

uaacaaugaa uaacaaacac uggcuaguac auaaagaaug guuucacgau auaccauuac      780

cauggcaugc aggagcagau acaggaacac cacauuggaa uaauaaagaa gcacuaguag      840

aauuuaaaga ugcacacgcu aaaagacaaa ccguuguagu auuaggauca caagaaggag      900

cgguucacac agcacuagcc ggagcauuag aagcggaaau ggauggagca aaaggaagau      960

uaucaucagg ucacuuaaaa uguagguuaa aaauggauaa acuaagacua aaaggaguaa     1020

gcuauagucu uuguacagca gcauucacau ucacaaaaau accagcagaa acauuacaug     1080

gaacaguaac aguugaagua caauaugcag gagcagacgg accaugcaaa guaccugcac     1140

aaauggcagu ggauaugcaa acauuaacuc caguagguag guuaauaaca gcaaacccag     1200

uaauaacaga aaguacggaa aauucaaaaa ugaugcuaga auuagaucca ccauuuggag     1260

auaguuauau aguaauagga guaggagaaa aaaaaauuac acaccauugg cauagaucag     1320

guaguacaau aggaaaagca uuugaagcaa ccguacgugg agcuaaaaga auggcuguau     1380

uaggagauac agcaugggac uuugguaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7143
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 2 opt

<400>  7143
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auaucuaguu augauacuau uaauagcacc agccuacagu auacgaugua      120

uuggaguaag uaauagagau uuuguugaag gaaugagugg gggaacaugg guagaugugg      180

uacuugaaca uggaggaugu gucacaguaa uggcacaaga uaaaccaaca guggauauag      240

aauuaguaac aacuacagua aguaacaugg cagaaguaag aaguuauugc uacgaagcuu      300

caauaaguga cauggcaagu gauagcagau guccgacaca aggcgaagca uaucuagaua      360

aacaaagcga uacucaauau guauguaaaa gaacauuagu agacagaggu uggggcaaug      420

gauguggauu auuugguaaa ggcucucuag uaacaugugc aaaauuugca uguucaaaaa      480

aaaugacagg aaaaucaauu caaccagaaa aucuagaaua uagaauaaug uuaucagucc      540

auggaaguca acauucaggu augauaguaa acgacacagg acaugaaaca gaugaaaaca      600

gagcaaaagu agaaaucaca ccaaauaguc caagagccga agcaacuuua ggaggauucg      660

gaucauuagg auuagauugc gaaccaagaa ccggguuaga uuuuagugau uuauauuauc      720

uuacuaugaa uaauaaacau uggcuaguac auaaagaaug guuucaugau auaccauuac      780

cuuggcacac uggagcagau acaggaacac cacauuggaa caauaaagaa gcuuuaguag      840

aauuuaaaga ugcacaugca aaaagacaaa caguaguagu auuaggaagu caagaaggag      900

cuguacauac ugcauuagcu ggugcauuag aagcagaaau ggauggagca aaaggaaggc      960

uaagcagugg acaucuaaaa uguagauuaa aaauggauaa acuaagauua aaaggaguau     1020

cauauagucu auguacagca gcauucacau uuacaaagau accagcagaa acacuacacg     1080

guacaguaac gguagaaguc caauaugcag gaacagaugg cccuuguaaa gugccagcac     1140

aaauggcagu agauaugcaa acacuaacuc caguaggaag acuuauaaca gcaaauccag     1200

uuauaacaga aggcacagaa aacaguaaaa ugauguuaga acucgaucca ccauuuggcg     1260

auucauauau aguaauagga guaggagaaa aaaaaauaac acaucauugg cauaggucug     1320

gaucaacuau aggaaaagca uuugaggcaa cuguaagagg agcaaaaaga auggcaguuu     1380

uaggugauac agcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7144
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 2 opt

<400>  7144
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auauuuagua augauauugu uaauagcacc agcguacagu auuagaugua      120

uaggaguuag caauagagau uuuguagaag gaaugagugg aggaaccugg guagauguag      180

uauuagaaca ugggggaugu guaacagcaa uggcgcaaga uaaaccaaca guagacauag      240

aauuaguaac aacuaccguu aguaauaugg cagaaguacg aagcuauugu uacgaagcuu      300

caauaaguga uauggcaagu gacagcagau guccaacaca aggagaagcg uaccuagaua      360

aacaaaguga uacacaauac guaugcaaaa ggacuuuagu agauagagga uggggaaaug      420

gaugugguuu auuugggaaa ggaagccuug uaacaugugc aaaauuugca uguagcaaaa      480

aaaugacagg uaaaucaaua caaccugaaa aucuagaaua uagaauaaug uuaagcguac      540

auggaucaca acauagcgga auguuaguaa acgauacagg acaugaaaca gaugaaaaua      600

gagcaaaagu agaaauaacg ccaaacucac cucgagccga agcaacauua gguggauuug      660

gaucauuagg auuagauugc gaaccaagaa caggcuuaga cuuuagcgau uuauauuacu      720

uaacgaugaa caauaaacau ugguuagcuc auaaagaaug guuucaugau auaccacuac      780

cauggcacgc gggagcagca acaggaacgc cucauuggaa uaauaaagaa gcauuaguag      840

aauuuaaaga ugcacaugcu aaaagacaaa caguaguagu acuaggaagc caagaaggag      900

caguacacac agcauuggca ggagcauugg aagcagaaau ggauggagca aaaggacgau      960

uaaguucugg acauuuaaaa ugucgauuaa aaauggauaa acuaagacuc aaagggguau     1020

cauacucauu auguacagca gcguuuacau ucacaaaaau accagcagaa acaguagaug     1080

guacaguaac aguagaagga caauauggug gaacagacgg accaugcaaa gugccagcac     1140

aaauggcagu agauaugcaa acacuuacac caguaggaag auuaauaaca gcaaauccag     1200

ugauaacaga aucgacagaa aacaguaaaa ugauguuaga acuagaucca ccuuuuggag     1260

auucauauau uguaauagga guaggggaaa aaaaaauaac acaucauugg cauagaucug     1320

gaagcacgau aggaaaagcu uuugaagcaa caguaagagg agcaaaaaga auggcaguuu     1380

uaggggauac agcaugggau uucggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7145
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 2 opt

<400>  7145
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguuau auaccuagua augauauuau uaauagcacc agccuauagu auacgaugca      120

uuggaguauc aaauagagau uucguagaag gcaugucagg uggaacaugg guagauguag      180

uauuagaaca uggaggaugu guaacaguaa uggcacaaga caaaccagca guagauauag      240

agcuaguaac aacuacagua ucaaauaugg cagaaguaag aaguuacugu uaugaagcua      300

gcauaagcga uauggcaucu gauucuaggu guccaacaca aggcgaagca uauuuagaua      360

aacaaucuga uacacaauac gucuguaaaa gaacucuagu agauagagga uggggaaacg      420

gaugugguuu auucggaaaa ggcagcuuag uuacaugugc aaaauuugca ugcucaaaga      480

aaaugacagg aaaaucaaua caaccugaaa accuagaaua cagaauaaug uuaucagugc      540

acggaucaca acauagcgga augauaguca augauacagg acaugaaaca gaugaaaaca      600

gagcuaaagu cgaaauaaca ccaaacaguc caagagcuga agcaacauua ggaggauucg      660

guagcuuagg acuugauugc gaaccaagaa caggacuaga uuuuucagau cuuuauuacu      720

uaaccaugaa caacaaacau ugguugguac acaaagaaug guuucaugau auaccacuac      780

cuuggcaugc aggagcagau acuggaacac cacauuggaa caauaaagaa gcauuaguag      840

aauuuaaaga cgcucaugca aaaagacaaa cagucguugu auuaggauca caagaaggag      900

caguacauac cgcucuagcu ggagcauuag aagcagaaau ggauggagca aaaggaagau      960

uaucaucagg acacuuaaaa ugcagacuaa aaauggacaa auuaagacuc aaaggaguau     1020

cuuauucauu augcaccgca gcauuuacau uuacaaaaau accagcagaa acacuacaug     1080

gaacuguaac agucgaaguc caauaugcag gaacagaugg accgugcaaa gucccggcac     1140

aaauggcagu agacaugcaa acuuuaacac caguaggacg auuaauaacu gcaaauccug     1200

uaauaacaga aagcacagaa aauucaaaaa ugaugcuaga auuagaucca cccuucggag     1260

auucauauau cguaauagga guaggagaaa agaaaauaac acaucauugg cauagaucag     1320

gaagcacuau agguaaagca uuugaagcaa caguaagagg ugcaaaaaga auggccguuu     1380

uaggugauac agcaugggau uuuggcaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7146
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 2 opt

<400>  7146
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaaguaau auauuuagug augauacuau uaauagcacc agcauauagu auaagaugua      120

uaggugugaa uaauagagau uuuguugaag gaaugucagg gggaacaugg guagaugucg      180

ucuuagaaca cggagguugu guaacaguga uggcacaaga uaaaccaaca guagauauag      240

aauuaguaac aaccacagua aguaauaugg cagaaguaag aucauauugu uaugaggcaa      300

guauaaguga uauggcaagu gauaguagau guccgacaca gggagaagca uauuuagaua      360

aacaaagcga uacucaauau guauguaaaa gaacauuagu agauagagga uggggaaaug      420

gaugcggauu guuuggaaaa ggaagucuag ugacaugugc aaaauuugca ugcuccaaaa      480

aaaugacagg aaaaucaaua caaccagaga acuuagaaua uagaauaaug uuaaguguac      540

auggaaguca acacagcgga augauaguua augauacagg acaugaaaca gaugaaaauc      600

gagcgaaagu ugaaauaacu ccaaacucac cuagagcaga agcaacauua ggaggguucg      660

gaucauuagg acuagauugu gaaccaagaa caggacuaga uuucagugac uuauauuauc      720

uaacaaugaa uaauaaacau uggcuaguac auaaagaaug guuucaugau auacccuuac      780

cauggcaugc gggugcagau acagguacac cacauuggaa uaauaaagaa gcccuugugg      840

aauuuaaaga ugcacacgca aaaagacaaa caguagucgu acuagguuca caagaaggag      900

ccgugcacac agccuuagca ggagcacugg aagcagaaau ggauggggca aaaggaagau      960

uaucgagugg acaucuaaaa ugcagacuaa aaauggauaa auuaagauua aaaggaguaa     1020

guuauucauu auguacagca gcauuuacau uuacaaaaau accagcagaa acauuacaug     1080

gaacaguaac aguugaagua caauaugcgg gaacagacgg accauguaaa guaccagcac     1140

aaauggcagu agacaugcaa accuuaaccc cagucggaag acuaauaaca gcaaacccgg     1200

uaauaaccga aaguacagaa aacucuaaaa ugauguuaga acuagaucca ccauuuggag     1260

auaguuacau aguaauagga gugggagaaa agaaaauaac acaucacugg cauagaagcg     1320

gaagcacaau uggcaaagca uucgaagcaa ccguaagagg agcaaaaaga auggcaguau     1380

uaggagauac ugcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7147
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 2 opt

<400>  7147
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auauuuaguc augauauugc uaauagcacc ugcauauagu auaagaugua      120

uaggaguaag uaauagagac uuuguagaag gaaugagcgg cggaacaugg guagacguag      180

uacuagaaca uggaggaugu guaacaguaa uggcacaaga uaaaccaaca guagauauag      240

aauuaguaac aacaacagua ucuaacaugg cugaaguaag aaguuauugc uacgaagcaa      300

guauaagcga uauggcauca gauuccagau guccgacaca aggagaagca uauuuagaua      360

aacaaagcga uacacaauau gugugcaaaa gaacauuagu agauagagga uggggaaaug      420

gaugcggacu auuuggaaaa gguagcuuag uaacaugugc aaaauuugca uguucuaaaa      480

aaaugacagg aaaaagcaua caaccagaaa auuuagaaua ucgaauaaug uuaagcguac      540

auggaucaca gcauucagga augauaguaa acgauacagg ucaugaaaca gacgaaaaua      600

gagcaaaagu agaaauaaca ccuaacuccc caagagcaga agcaacauua ggaggauuug      660

gaucacuagg auuagauugu gaaccaagaa cuggacuuga cuucagugac uuauauuauu      720

uaacaaugaa uaauaaacau ugguuaguac auaaagaaug guuucacgac auaccauuac      780

cauggcaugc aggagcagau acaggaacuc cccauuggaa uaauaaagaa gcauuaguag      840

aauuuaaaga ugcacacgca aaaagacaaa caguaguagu acuuggaagu caagaaggag      900

caguacauac agcuuuagca ggagcacugg aagcagaaau ggacggugcu aaaggaagac      960

uuucaagugg acaucuaaaa ugucgguuaa aaauggauaa auuaagauua aaaggcguaa     1020

guuauagcuu augugcagca gcauuuacuu uuacaaaaau accugcagaa acacuacacg     1080

guacaguuac gguagaaguc caauaugcug gaacggaugg accauguaaa guaccugcac     1140

aaauggcagu agauaugcag acauuaacuc cagugggaag auuaaucaca gcaaauccag     1200

uaauaacaga aaguacagaa aauaguaaaa ugaugcuaga auuagaucca ccauucgggg     1260

auaguuauau aguaauagga guaggugaaa aaaaaauaac acaucacugg cauagauccg     1320

gaucgacaau aggaaaagca uuugaagcaa caguaagagg ggcuaaaaga auggcaguau     1380

uaggagauac agcuugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7148
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 2 opt

<400>  7148
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auaccuagua augauauuau uaauagcacc agcauauagc auaagaugua      120

uaggaguaag uaauagagac uuugucgaag gaaugagugg aggaacaugg guagacguag      180

uauuagaaca uggaggaugu guaacaguua uggcacaaga uaaaccaaca guagacauag      240

aacuaguaac aacuacaguu ucaaauaugg cugaaguaag aaguuauugu uacgaagcaa      300

guauuagcga uauggcgagu gauucaagau gcccaacuca aggagaagca uaucuagaua      360

aacaaaguga uacacaauau guauguaaaa gaacacuagu agacagagga uggggaaaug      420

gauguggauu auuuggaaaa ggaucauuag uuacaugugc aaaauuugca uguucaaaaa      480

aaaugacagg aaaaaguaua caaccugaaa auuuggaaua uagaauaaug cugaguguac      540

auggaucaca acacagugga augauaguca augauauugg acaugaaaca gaugaaaauc      600

gagcaaaagu agaaauaaca ccgaauagcc caagagcaga agcaacuuua ggaggauuug      660

gaucauuagg gcuagauugu gaaccuagaa cuggacuaga uuuuagugac uuguauuacu      720

uaacaaugaa uaacaaacac uggcugguac auaaagaaug guuucaugau auaccauuac      780

cauggcacgc aggugcagac acaggaacac cacauuggaa uaauaaagaa gcauuaguag      840

aauucaaaga ugcacaugca aaaagacaaa cagugguagu auuaggaagu caagaaggag      900

caguacauac ugcauuagca ggugcuuuag aagcagaaau ggauggagca aaaggaagau      960

uaucuucagg acauuuaaaa ugcagauuaa aaauggacaa auuaagguua aaaggaguaa     1020

guuacagccu uuguacagca gcauuuacuu uuacgaaaau ccccgcagaa acuuuacaug     1080

gaacaguaac aguagaagua caauaugcag gaacagacgg accaugcaaa guaccagcac     1140

aaauggcagu cgauaugcaa acauuaacac cuguaggaag acucauaaca gcgaauccgg     1200

uuauuacuga aucaacagaa aauaguaaaa ugaugcuaga auuagaccca ccauuuggag     1260

auucguauau aguaauagga guaggcgaua aaaaaauaac acaucauugg cauagaucag     1320

gaucaacaau aggaaaagca uuugaagcaa caguaagagg agcaaaaaga auggcaguau     1380

uaggagauac agcaugggac uuuggaucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7149
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 2 opt

<400>  7149
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguaau auauuuagua augauucuau uaauagcucc ugcauauuca auaagaugua      120

uagguguaag caauagagau uuuguagaag gaaugagugg aggaacgugg guagauguag      180

uauuagaaca cggaggaugu guaacuguaa uggcacaaga uaaaccaacu guagauauag      240

aauuaguaac aacuacagua ucaaauaugg ccgaaguaag aagcuauugc uaugaagcua      300

guauaaguga cauggcauca gacucaagau guccaacaca aggcgaagca uaccuagaua      360

aacaaaguga uacacaauau gucugcaaaa ggacgcuagu agauagagga uggggaaaug      420

gauguggucu auuuggaaaa gggucauuag ucacaugugc aaaauuugca uguucaaaga      480

aaaugacagg aaaaucaaua cagccagaaa auuuagaaua uagaauuaug uuaaguguac      540

acggaagcca acacucagga augauuguaa acgacacagg acaugaaacc gaugaaaaca      600

gagcaaaagu agaaauaaca ccuaauucac caagagcaga agcaacguua ggaggauuug      660

guucuuuagg acuagauugu gaaccaagga caggacuaga uuuuagugac uuauauuacu      720

uaacaaugaa caauaaacau uggcuaguuc auaaagaaug guuucaugau auaccucuac      780

cauggcacgc cggagcagau acuggaaccc cacauuggaa uaauaaagaa gcauuaguag      840

aauuuaaaga ugcacacgca aaaagacaaa cuguaguagu auuagguucu caagaaggug      900

caguacacac agcuuuagca ggagcauuag aagcagaaau ggauggugca aaaggaagac      960

uaaguagugg acacuuaaaa uguagauuaa aaauggauaa acuaagauua aaaggaguau     1020

cauauucauu auguacugca gcauucacau ucacaaaaau uccagcagaa acuuuacaug     1080

gaacaguuac aguugaagua caauaugcag gaacagaugg accaugcaaa guaccagcgc     1140

aaauggcagu agauaugcaa acauuaacuc caguaggacg auuaauaacc gcaaauccag     1200

uaauaacaga aaguacagaa aacaguaaaa ugaugcuuga auuagaccca ccauuuggag     1260

auucauauau uguaauaggu guaggagaca agaaaauaac acaccacugg uacagaucgg     1320

gaucaacgau aggaaaagca uuugaagcaa caguaagagg agcaaaaaga auggcaguau     1380

uaggagacac agcaugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7150
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 2 opt

<400>  7150
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaagugau auauuuagua augauauuac uaauagcacc agcauacagu auaagaugua      120

uaggaguauc uaauagagau uuuguagaag gaaugucagg aggaacaugg guagauguag      180

uacuagaaca cggaggaugu guaacaguaa uggcacaaga caaaccaaca guagauauag      240

aauuaguaac aacaacugua agcaacaugg cagaaguuag aaguuauugu uaugaagcaa      300

guauaaguga uauggcaagu gauucaagau guccuacaca aggagaagca uacuuagaca      360

aacaaagcga uacacaauau guuugcaaaa gaacguuagu cgaccgagga ugggggaacg      420

gauguggacu auuuggaaaa ggaagucuag uaacaugugc uaaauuuaca uguagcaaaa      480

aaaugacagg aaaaucaaua caaccagaaa auuuagaaua cagaauaaug uuaagugugc      540

auggaucaca acauagcgga augauaguua augauacugg gcacgaaacu gaugaaaaua      600

gagcgaaagu agaagucaca ccaaacaguc caagagcgga agcaacuuua ggaggauucg      660

guucauuagg acuagauugc gaaccuagga cuggauuaga uuuuucagau cuauauuauc      720

uuacaaugaa uaauaaacau ugguuaguac auaaagaaug guuucaugau auaccacuac      780

cguggcaugc aggagcagau acaggaacac cucauuggaa uaacaaagaa gcauuaguag      840

aauucaaaga ugcacaugca aaacgacaaa cgguaguagu acuuggaagu caagaaggag      900

caguacacac agcauuagca ggagcuuuag aagccgagau ggacggggca aaaggcagac      960

uauuuagcgg acacuuaaaa ugcagauuaa aaauggauaa auuaagauua aagggaguaa     1020

guuauucauu auguacagcu gcauuuacuu uuacaaaagu uccagcagag acauuacaug     1080

gaacaguaac aguggaagua caauaugcag gaacagaugg gccauguaaa guuccagcac     1140

aaauggcagu agauaugcaa acauuaacuc caguaggaag acuuauuaca gcaaauccag     1200

uaauaacaga aaguacagaa aauaguaaaa ugaugcuuga auuagaccca ccauuugggg     1260

auucuuauau agucauagga guuggagaua aaaaaauaac ucaucauugg cauagaucug     1320

gcucaacuau aggaaaagcg uuugaagcaa cgguaagagg agcaaaaaga auggcuguac     1380

uaggcgacac agcuugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7151
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 2 opt

<400>  7151
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguuau auauuuagua augauauugu uaauagcacc ggcauauagu auaagaugua      120

uaggaguauc aaauagagau uuugucgaag gaaugagugg aggaacaugg guugauguag      180

uauuagaaca uggcggaugc guaacuguaa uggcacaaga uaaaccaacu guggauauag      240

aauuaguaac aacaacagua agcaauaugg cagaaguaag aaguuauugu uaugaagcau      300

caauaaguga uauggcaagu gauucucgau gcccgacaca aggagaagca uacuuagaua      360

aacaaaguga uacacaauau guaugcaagc gaacacuugu agacagagga uggggaaacg      420

gaugugguuu auucgguaaa ggaaguuuag uaacuugugc aaaauuuaca uguagcaaaa      480

aaaugacagg aaaaucaaua caaccugaga auuuagaaua uagaauuaug cuaagcguac      540

acgggucaca acauagcgga augauaguaa augauauagg acaugaaaca gaugaaaaua      600

gagcaaaagu agaaguaacu ccaaauaguc caagagcaga agcaacauua gggggcuucg      660

guucauuagg uuuagacugc gaaccacgga cuggacuaga cuucucagau uuauauuauu      720

uaacaaugaa uaacaaacac uggcugguac auaaagaaug guuucacgau auaccuuuac      780

cauggcaugc gggcgcagau acaggaacac cucauuggaa uaacaaagaa gcacuaguag      840

aauuuaaaga ugcacaugca aaacgacaaa caguaguagu auuaggaagu caagaagggg      900

caguacauac agcacuagcg ggagcauuag aggcagaaau ggauggagca aaaggaaggu      960

uauucagcgg acauuuaaaa uguagacuua aaauggauaa auuaagauua aaaggaguaa     1020

gcuauaguuu auguacagca gcauuuacau uuacaaaagu accagcagaa acauuacaug     1080

gcacaguaac cguagaagua caauaugcag gaacagaugg accaugcaaa guaccagcuc     1140

aaauggcugu agauaugcaa acacuaacac cuguaggaag acugauuaca gcuaauccag     1200

ucauaacaga aaguacggaa aauagcaaaa ugauguuaga acuagaucca ccauuugggg     1260

auagcuacau aguaauagga guaggagaua aaaaaauaac acaucacugg cauagaucag     1320

gaucaacaau aggaaaagca uuugaagcaa caguaagagg agcaaaacgc auggcaguau     1380

uaggggacac agcuugggau uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7152
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 2 opt

<400>  7152
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaaguuau auauuuaguu augauauuac uaauagcacc agcauauuca auaagaugua      120

uaggaguaag caacagagau uuuguugaag gaaugagcgg uggaacaugg guagacguag      180

uauuagaaca uggaggaugu guaacaguaa uggcacaaga uaaaccaaca guagacauag      240

aacuaguaac aacaacaguu ucaaauaugg cagaaguaag aaguuauugc uaugaagcaa      300

guauaaguga cauggcaagu gauaguagau guccaacuca aggugaagca uacuuagaua      360

aacagaguga cacacaauac guauguaaac gaacucuagu agacagaggc uggggaaaug      420

gaugugggcu uuucggaaaa ggaucauuag uuacgugugc aaaauuuaca ugcaguaaaa      480

aaaugacagg aaaaucaaua caaccagaaa aucuggaaua uagaauaaug uuaagcguac      540

auggaaguca acauucagga augauaguaa acgauaucgg acaugaaaca gaugaaaaca      600

gagcaaaagu agaaguaacu ccaaauagcc caagagcaga agcaacauua ggaggauuug      660

gaucauuagg ucuagauugu gaaccaagaa caggacuaga uuuuagugau cuauacuauc      720

uaacaaugaa uaauaaacau ugguuaguac acaaagaaug guuccaugau auaccauuac      780

ccuggcaugc aggagcagac acaggaacac cacauuggaa caauaaagaa gcauuaguag      840

aauuuaaaga cgcacaugca aaaagacaaa cuguaguagu gcuaggaagu caagaaggag      900

cuguacacac agcacuagca ggugcauuag aggcagagau ggauggagca aaaggaagau      960

uauuuagugg acaucuaaag uguagacuaa aaauggacaa auuaagauua aaaggaguaa     1020

guuauagucu gugcacagcu guaugcacag cagcaaaagu accagcagaa acuuuacaug     1080

gaacaguaac uguagaagua caauaugcag ggacagaugg uccuuguaaa guaccagcuc     1140

aaauggcagu agacaugcaa acgcuaacac caguaggaag acuaaucaca gcaaauccag     1200

uuauaaccga aaguacagaa aauagcaaaa ugauguuaga acucgauccu ccauuuggag     1260

auagcuauau cguaauagga guaggagaca aaaaaauaac ucaucauugg cauagaagug     1320

gaucaacaau aggaaaagca uuugaagcaa caguaagagg ugcaaaaaga auggcaguac     1380

uaggagacac agcaugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7153
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 2 opt

<400>  7153
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau auacuuaguu augauauuac uaauagcacc ggcuuauagu auaagaugca      120

uaggaguaag caauagagau uuuguagaag gaaugagcgg aggaacaugg guagauguag      180

uauuagaaca cggaggcugc guaacaguaa uggcucaaga uaaaccaacu guagauauag      240

aacuaguaac aacuacagua ucaaauaugg cagagguaag aaguuauugu uaugaagcau      300

caauaagcga uauggcauca gacaguagau guccaacaca aggugaagca uauuuagaua      360

aacaaaguga uacacaauau guauguaaaa gaacccuagu agacagggga uggggaaaug      420

gcuguggauu auuuggaaaa ggaucacuag uuacaugugc aaaauucaca uguagcaaaa      480

aaaugacagg aaaaucaaua caaccagaaa acuuagaaua cagaauaaug uuaaguguac      540

acggaagcca acauucaggu augauaguaa augaugaaaa cagagcaaaa guagaaguua      600

caccaaauuc accaagagca gaagcaacac uaggaggauu ugguucucuu ggauuagauu      660

gugagccaag aacaggucug gauuuuagug acuuauauua cuuaacaaug aauaauaaac      720

auugguuagu ucacaaagaa ugguuccaug auauucccuu accauggcau ucaggagcag      780

auacagaaac accacauugg aauaauaaag aagcauuagu agaauuuaaa gaugcacaug      840

caaaaagaca aacaguuguu guacugggaa gccaagaagg ggcaguacau acagcauuag      900

cuggagcacu agaagccgaa auggauggag cgaaaggcag acuaucaagu ggacauuuaa      960

aauguagauu aaaaauggau aaauuaagau uaaaaggagu aagcuacuca uuauguacag     1020

cagcauucac auuuacaaaa guaccagcag aaacauuaca ugguacagua acaguagaag     1080

uccaauaugc uggaagagau ggaccaugua aaguaccagc acaaauggca guagauaugc     1140

aaacauuaac accaguuggu agauuaauaa cagcuaaucc agugauaacu gaaucaacag     1200

aaaauaguaa gaugauguua gaacuagauc caccauucgg agacaguuau auaguaauag     1260

gagucggaga uaaaaaaaua acgcaucauu ggcauagaag cggaaguauu auaggaaaag     1320

cauucgaagc aacaguaaga ggagcaaaaa gaauggcggu acuaggagau acagcuuggg     1380

auuuugguag uugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  7154
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 2 opt

<400>  7154
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguaau auaucucguu augauaguuc uaauaguacc agcuuacagu auaagcugua      120

uaggaguauc aaauagagac uuaguggaag gcaugagcgg agguacaugg guagacguag      180

uauuagaaca cggaggaugc guaacagaaa uggcccaaga uaaaccaacu guggauauag      240

aauuaguaac gaugacagua agcaauaugg cugagguaag aagcuauugu uacgaagcaa      300

guuuaucaga uauggcuagc gcaucaaggu guccaacaca aggcgaacca ucauuagaua      360

aacaaaguga uacucaaagu guauguaaaa gaacucuagg agauagggga uggggaaaug      420

gaugcggaau auucgguaaa ggaagucuag uaacaugcag uaaauucaca uguuguaaaa      480

aaaugccugg aaaaucaauu caaccagaaa auuuagaaua cagaauaaug cuaccaguac      540

auggaaguca acauagcgga augauaguua augauauagg acaugaaacu gaugaaaaca      600

gagcaaaagu agaaguaacu ccaaacagcc cuagagcaga agcaacacuc ggaggauucg      660

gaucacucgg auuagauugu gaaccaagaa caggacuuga cuucagugau cuuuacuauu      720

uaacgaugaa uaacaaacau ugguuaguac auaaagaaug guuccaugau auaccauuac      780

cauggcaugc aggagcagau acaggaacac cacauuggaa caauaaagaa gcguuaguag      840

aauuuaaaga ugcacaugca aaaagacaaa caguaguagu auuaggaagc caagaaggag      900

cuguacacac ggcacuagca ggggcauuag aagcagaaau ggauggagcc aaagguagau      960

uauuuagugg acauuuaaaa uguagauuaa aaauggauaa acuaagacua aaaggaguaa     1020

guuacagcuu auguacagca gcauuuacau ucacaaaggu accagccgaa accuuacacg     1080

gaacaguuac aguagaaguc caaagugcag gcacagaugg uccauguaaa guaccggcac     1140

aaauggcagu agauaugcaa acuuuaacac cuguaggaag guuaauaaca gcaaauccag     1200

uaauaacaga aagcacagaa aauaguaaaa ugaugcuaga auuagaccca ccauuuggag     1260

auucauauau aguaauagga gucggagaua aaaaaauaac acaucauugg cacagaagug     1320

gaucaacaau aggaaaagca uuugaagcaa caguaagagg cgccaaaaga auggcaguau     1380

uaggagauac agcaugggau uuuggaucuu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7155
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 2 opt

<400>  7155
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

aaaaaguaau auauuuagua augauauuac uaauugcacc ugcauauuca auaagaugca      120

uagguguuag uaauagagau uuuguugaag gaaugucagg aggaacuugg guagacguag      180

uacuagaaca ugguggcugu guaacaguaa uggcacaaga uaaaccaaca guagauauag      240

agcuaguuac gacaacaguu agcaacaugg cagagguaag aaguuauugu uaugaagcaa      300

guauaucaga cauggcaagc gauucaaggu guccaacaca aggugaagca uacuuagaua      360

aacaaaguga uacacaauac guauguaaaa gaaccuuagu ugauagaggu uggggaaaug      420

guuguggacu auuuggaaaa ggaaguuuag uuacaugcgc aaaauuugca uguaguaaaa      480

aaaugacagg aaaaucaaua caaccagaga auuuagaaua uagaauaaug cuuaguguac      540

auggaaguca acauagcgga augauaguaa augacacagg acaugaaacu gaugaaaaua      600

gagcaaaagu agaaauaaca ccaaauucac ccagagcaga agcuacauua ggaggauuug      660

gaucauuagg auuagacugu gaaccuagaa ccggauuaga uuuuucagau cuauauuacu      720

uaacaaugaa uaauaaacau ugguuaguac auaaagaaug guuucaugau auaccguuac      780

cauggcaugc aggcgcagau acaggaaccc cacacuggaa uaauaaagaa gcacuaguag      840

aauuuaaaga ugcccaugca aaacgucaaa cuguaguagu acuaggauca caagaaggag      900

caguacacac ugcauuagcc ggagcuuuag aagcagaaau ggauggagcg aaaggaagac      960

uaucaucugg acaccuaaaa uguagauuaa aaauggauaa auuaagauua aaaggaguaa     1020

guuauaguuu augcacagca gcauucacau uuacgaaaau uccagcagaa acauuacacg     1080

gaacaguaac aguagaaguu caauaugcgg gaacagacgg accauguaaa guaccagcac     1140

aaauggcagu ugauaugcag acauugacuc ccguuggaag acuaauaaca gcaaauccag     1200

uaauaacuga aucaacagaa aacucaaaaa ugaugcuaga auuagaucca ccauuuggag     1260

auucauauau aguaauagga guaggugaaa aaaaaauaac ucaucauugg cacagaagug     1320

ggaguacaau aggaaaagca uuugaagcaa cuguaagagg ggcaaaacga auggcaguac     1380

uaggagauac ugcaugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7156
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 2 opt

<400>  7156
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaagaguaau auacuuagua augauauuau ugauagcacc agcauauuca auuagaugua      120

uaggaguaag uaauagagau uuuguagaag gaaugagugg aggaacaugg guagauguag      180

uauuagaaca uggaggaugu guaacuguua uggcacaaga uaaaccaaca guagauauag      240

aauuaguaac aacaacagua ucaaauaugg cagaaguaag aagcuacugu uacgaagcau      300

caaucucuga uauggcaagu gauaguagau guccgacaca aggagaagcu uauuuagaua      360

aacaaagcga uacacaauau guaugcaaaa gaacauuagu ugauagagga uggggaaaug      420

gauguggauu auuuggaaaa gguagucuag uaacaugugc uaaauuuaca uguagcaaaa      480

aaaugacagg aaaaaguaua caaccagaaa aucuagaaua uaggauaaug cuaucaguac      540

acggaaguca acacagcgga augauuguaa augacauagg acacgaaaca gaugaaaauc      600

gagcaaaagu agaaguaacu ccaaauucac cuagagcaga agcaaccuua ggagguuuug      660

ggucauuagg acuagacugu gaaccaagaa caggauuaga uuuuagugac uuauauuacu      720

uaacaaugaa uaauaaacau uggcuaguac acaaagaaug guuucacgau auaccacuac      780

cauggcacgc aggagcagau acuggaacac cacauuggaa uaauaaagaa gcauugguug      840

aauuuaaaga ugcacaugcu aaaagacaaa ccguaguagu guuaggcagc caagaaggag      900

caguacauac agcuuuagca ggagcauuag aagcagaaau ggauggagca aaagguagau      960

uauuuucagg acaucugaaa uguagaauaa aaauggauaa auuaagauua aaaggaguau     1020

ccuauaguuu auguacagca gcauucacgu uuaguaaagu accagcagaa acguuacaug     1080

guacaguaac aguagaagua caauaugcag guagggacgg accguguaaa guaccagcac     1140

aaauggccgu agauaugcaa acauuaacac caguaggacg auuaauaaca gcaaacccaa     1200

uaauaacaga aucaacugaa aauaguagaa ugauguuaga auuagaucca ccauuuggag     1260

auucauauau aguuauaggu guaggagaua aaaaaauaac ccaucauugg cauagaagug     1320

gcucaacaau agguaaggca uuugaagcga caguaagagg agcgaaaaga auggcugugc     1380

uaggagauac ugcaugggau uuuggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7157
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  7157
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgua       60

uaaucaacgc acgaaaagag aagaaaaggc gcggcgccga cacuucaguc gguaucgucg      120

gccuccuccu cacaaccgcc auggccgcag aagucacccg ccgaggaagu gcauacuaca      180

uguaccuaga ccgcaaugac gccggagaag caaucagcuu ccccaccaca cucgguauga      240

acaaaugcua cauacaaaua auggaccucg gucacaugug cgacgccacg augagcuacg      300

aaugccccau gcucgacgaa ggggucgaac cagacgacgu ugacugcugg ugcaacacaa      360

ccucuacuug ggucgucuac gguaccugcc accacaagaa aggcgaagcg cgacgauccc      420

gcagggccgu cacccugccg agccauucaa cccgaaaacu acaaacccga agccaaaccu      480

ggcucgaauc ucgcgaauac accaaacacu ugauccgcgu cgaaaacugg auuuucagaa      540

acccaggcuu cgcacucgcg gcagccgcaa uagccuggcu ccucggcuca ucaacauccc      600

agaaagucau cuaccucgua augauucuac ucaucgcccc agcauacuca auacggugca      660

uaggaguguc caaccgcgac uucgucgagg gaaugucagg cggcacaugg guagacauag      720

uacucgaaca cggaggaugc gucaccguaa uggcccaaga caaaccaacc guugacauag      780

aacucguaac cacaaccgua agcaacaugg cagaaguccg cucauacugc uacgaggcau      840

ccaucagcga cauggccucc gacagccgau gccccaccca aggagaggcc uaccucgaua      900

aacaaagcga cacccaauac gucugcaaaa gaacccucgu agauagaggu uggggcaacg      960

gcugcggucu auucggcaaa ggaagccuag uaacaugcgc caaauucgcc ugcuccaaga     1020

aaaugaccgg caaauccauc caaccggaga accuugaaua uagaaucaug cuguccguac     1080

acggauccca acacuccgga augauaguca acgacacagg ccacgagacc gacgagaacc     1140

gagcaaaagu agagaucaca cccaacucgc caagagccga agccacccua gguggcuucg     1200

gaucacuugg ccucgacugc gaaccccgga cagggcucga cuucucagac cucuacuacc     1260

ucaccaugaa caacaaacac uggcuuguac acaaagaaug guuucacgac aucccacucc     1320

cauggcaugc aggcgcagac accggcacac cacacuggaa caacaaagaa gcccuagucg     1380

aguucaaaga cgcacacgca aaacgccaaa cagugguagu ccuggguucc caagaaggcg     1440

ccguccacac agcccucgcc ggcgcccucg aagcagaaau ggacggcgca aaaggacgcc     1500

uuagcuccgg ccaccuaaaa ugcagacuca aaauggacaa acuccgccuu aaaggcguau     1560

cauacagccu cugcacagcu gcauucaccu ucaccaaaau acccgccgaa acccuccacg     1620

gaacagucac cgucgaagua caauacgcag gaacggaugg cccuugcaaa guaccagccc     1680

agauggccgu cgacaugcag acccuaacac cagucggaag acucauaacc gccaauccag     1740

ucauaacaga guccaccgaa aacuccaaaa ugaugcucga acuagacccu ccauuuggcg     1800

acuccuacau agucauaggc guaggagaaa agaaaauaac acaccacugg caccgcuccg     1860

guuccaccau uggaaaagca uucgaagcga caguacgcgg ggcaaaacgu auggcggugc     1920

ucggcgacac cgccugggac uuuggguccg ucggcggcgc ccucaauuca cucggcaaag     1980

gaauacauca aaucuucgga gcggccuuca aaucccuauu cggcggcaug agcugguucu     2040

cccaaauacu gaucggcaca cuacucaugu ggcuaggccu caacaccaaa aacggcucaa     2100

ucucccucau gugccuggca cucggcggcg uacucaucuu ccuauccaca gcagugagug     2160

ccgacguggg augcuccgua gacuucucga aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7158
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 2 opt

<400>  7158
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgaaaagaa aagaaacgac gaggagcgga cacgucaguc ggcaucguag      120

gauuacugcu caccacagca auggccgccg aagucacccg caggggcagc gcauauuaca      180

uguaccuuga ccgaaacgac gcgggggaag caauaucguu ccccaccacg cuaggcauga      240

acaaaugcua cauccaaaua auggaccuag gccauaccug cgacgccaca augucauacg      300

aaugcccaau gcuagacgaa ggcguagaac ccgacgacgu agacugcugg ugcaacacaa      360

caagcacaug ggucgucuac gggacaugcc accacaagaa aggcgaagcu cgcagaagca      420

gacgcgccgu cacacugcca agccacucca cccgaaaacu acaaacccgc agccaaaccu      480

ggcucgaaag ccgcgaauac acaaaacacc ucauacgagu cgaaaacugg aucuuccgaa      540

accccggcuu cgcccuggca gcagcagcca uagccuggcu ccuaggcuca agcacaagcc      600

aaaaaguaau auaccuaguc augauccuac uaaucgcgcc agcguacuca auccgaugca      660

ucggugucag caaccgagac uucgucgaag gcaugucagg uggcaccugg gucgaugucg      720

uccuggaaca cggcgguugc gucacgguca uggcccagga uaaaccuacc guagacauag      780

aacuggucac cacgaccgua agcaacaugg cagaagugag gagcuauugc uacgaagcau      840

caauaucgga cauggccucu gauucgcgau gcccgaccca aggcgaggca uacuuggaua      900

aacaaucaga uacccaauac guauguaaac gaacgcuagu agaccgaggg ugggggaacg      960

guugcggacu cuuuggaaaa ggcucccuag uaaccugcgc caaauucgcg uguucaaaga     1020

aaaugacagg caaaucaaua cagccagaaa accucgaaua ccggauaaug cucuccgucc     1080

acggguccca acacagcggc augauaguca acgacacagg ccacgaaaca gacgaaaaua     1140

gggccaaagu cgaaauaaca ccaaacucac cacgagcgga agccacgcuc ggcggauucg     1200

gaucccuagg ccucgacugu gaaccccgga ccggccugga cuuuagcgac cuauacuacc     1260

ucacaaugaa caacaaacac uggcucgucc acaaagaaug guuucacgac aucccccugc     1320

cauggcacgc gggcgcagac acuggcacac cacacuggaa caacaaagaa gcucuugucg     1380

aguucaaaga cgcacacgcc aaaagacaaa cagucguggu acucggaucc caagaaggag     1440

ccguacacac cgcccucgcc ggcgcccucg aagccgagau ggacggugcc aaaggacgcc     1500

ucagcagcgg acaccuaaaa ugcagacuaa aaauggacaa gcucagacuc aagggcguau     1560

ccuacucccu cugcaccgcc gccuucaccu ucacaaaaau ccccgccgaa acccuacacg     1620

ggaccgucac cgucgaagua caguacgccg gcacagaugg ccccuguaaa guaccagccc     1680

aaauggccgu cgacaugcaa acccugacac ccgucggacg ccuaauaacg gccaaccccg     1740

ucaucaccga aucaaccgaa aacuccaaaa ugaugcucga acuagaccca cccuucggcg     1800

acagcuacau cgucaucggg gucggagaaa agaaaauaac ccaccacugg caccgauccg     1860

ggucaaccau cggcaaagca uucgaagcca ccguccgcgg cgcaaaacgc auggcggucc     1920

ugggagacac agcuugggac uucggauccg ucggcggcgc ccuaaacuca cucggaaaag     1980

gcauucacca aaucuucggc gccgccuuua aaagccucuu cggcggaaug ucgugguucu     2040

cccagauccu uauaggaacc cuacuaaugu ggcucggcuu gaacgccaaa aacggaagca     2100

uaagucugau gugccucgca cucggaggcg uccuaaucuu ccuaagcacc gcaguaagcg     2160

ccgacguugg auguagugua gacuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7159
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 2 opt

<400>  7159
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaacgc ccgaaaagaa cgcaaacgca gaggagccga cacaagcaua ggcauuaucg      120

gccuacuucu caccaccgca auggcugcag aaaucaccag acgaggaagc gccuauuaca      180

uguaucucga cagaucagau gcaggaaagg caaucuccuu cgccaccaca cucggaguaa      240

acaaaugcca cgugcaaauc auggaccucg gacacaugug cgacgcgaca augucauacg      300

agugccccau gcuagacgag ggagucgagc cagacgacgu agacugcugg ugcaacacaa      360

ccucaaccug ggucgucuac ggaacaugcc accacaaaaa gggggaagca cgccgaagcc      420

gcagagcggu cacacuacca agucacucca cacgcaagcu ccaaacccgg agccagaccu      480

ggcuagaauc ccgggaauac accaaacacc uaauaaaagu agagaacugg auauuucgga      540

acccaggauu cgcgcucguc gccguagcca ucgcauggcu acuaggcucc agcacaucac      600

aaaaagucau auaccuugua augauccucc ucaucgcccc cgcauacucc auccgcugca      660

uaggagucag caaucgcgac uucgucgaag gcaugucagg aggaaccugg guagauguag      720

uacucgagca uggcggcugc gucacaguca uggcacaaga caaaccaacc guagacaucg      780

aacucgucac aaccaccgug agcaacaugg cugaaguccg gucauacugc uacgaggcau      840

caauauccga cauggccucc gacagccgcu gcccaaccca aggcgaagcc uaccucgaca      900

aacagagcga cacacaauac gucugcaaac gaacccucgu agacagaggc ugggggaacg      960

gaugcggacu auucggcaaa ggaucccucg uaaccugcgc caaguucacg ugcagcaaga     1020

aaaugaccgg caaauccauu caacccgaaa accuagagua ccgcaucaug cuaucagucc     1080

acggcaguca acacucaggg augaucggcu acgaaaccga cgaagaccgc gccaaagugg     1140

aagucacacc aaacucacca cgcgccgaag caacacuagg cggguucgga ucccugggac     1200

ucgacugcga gccccgcacg ggccuagacu uuagcgaccu uuacuaucua accaugaaca     1260

acaaacacug gcuaguacac aaagaguggu uccacgacau cccacuaccg uggcacgcag     1320

gggcagauac cggcacaccg cacuggaaca auaaagaagc ccuaguagag uucaaagacg     1380

cacacgccaa acgacaaacc guaguggucu uaggauccca ggaaggagcc guacacaccg     1440

cccucgccgg agcccuagaa gccgaaaugg acggcgccaa aggccgccua uuuucgggcc     1500

accucaaaug ccgccugaaa auggacaaac uacgccuaaa agggguaagu uacucccucu     1560

gcaccgccgc auucacauuc acuaaagucc cagccgaaac ccuccacggc acaguaacag     1620

uagaagucca auacgccgga accgacggac ccugcaaaau ccccguacaa auggcaguag     1680

auaugcaaac ccuaaccccc gucggccgcc uaauaacagc caaucccgua auuaccgaau     1740

ccacagagaa cagcaagaug augcucgaac uugacccacc cuucggcgac ucguacauag     1800

ucaucggagu cggcgacaag aaaauuacac accacuggca ccggucagga ucgacaaucg     1860

gcaaagcguu cgaggcaacc guacgaggag ccaaaagaau ggccguacua ggugacaccg     1920

cgugggacuu cggcuccgua gggggcguau ucaacucccu aggaaaagga auccaccaga     1980

ucuucggcgc cgcauucaag ucacuauucg gggguaugag cugguucagc caaauccuaa     2040

ucggcacccu acuggugugg cucggucuca acaccaaaaa cggaucaauc ucccucacau     2100

gccuagcauu agggggaguc augaucuucc ucagcacagc agucucagcc gacgugggau     2160

gcagcguaga cuucucaaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  7160
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 2 opt

<400>  7160
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc caggaaagaa aagaaaagac gaggagcaga uaccagugua ggcauaguag      120

gcuuacuccu cacuacagca auggcagccg aaguaacaag acggggcucc gcguacuaca      180

uguaccucga ccgaaacgac gcaggagagg cgaucagcuu ccccacgacg cuagggauga      240

acaaaugcua cauacaaauc auggaccucg gccacaugug cgacgcaaca augagcuacg      300

aaugccccau gcuggaugaa ggaguggagc ccgacgacgu agacugcugg ugcaauacca      360

caaguaccug ggugguguac ggaacaugcc accacaagaa aggcgaagcc cgacgaucgc      420

gccgagccgu aacucugccg ucccauucga ccagaaaacu ccaaacaaga ucacagacau      480

ggcuagaguc acgggaauau acaaaacacc ucauccgcgu agaaaacugg auauuccgaa      540

acccgggcuu cgcccucgca gcggcagcaa ucgccuggcu gcuggggagu ucgaccagcc      600

aaaaagucau auaccuagua augauccuac ucaucgcacc ggcuuacagc auacgcugca      660

uaggagucuc aaaccgagac uucgucgaag gaaugagcgg cggcaccugg guagacguag      720

uacucgagca cggcgggugc guaaccguca uggcacaaga caaaccaaca guagacauug      780

agcuagucac gacaacagua uccaacaugg cugaaguaag aaguuacugu uacgaagcca      840

gcaucagcga cauggcaagc gacucucgcu gccccaccca aggagaagcc uaccucgaca      900

aacagucaga cacccaguac guauguaaac gaacgcuagu agacagaggc uggggcaacg      960

gauguggccu uuuuggaaaa ggauccuuag ucacaugcgc caaauucgcc ugcagcaaga     1020

aaaugacagg gaaaucaauc cagccagaaa accuagaaua ccggaucaug cucagcgucc     1080

acggcuccca gcacucggga augaucguaa acgacacagg ccacgaaaca gacgaaaauc     1140

gcgccaaagu agaaauaacc ccgaacagcc cccgcgcaga agccacucua ggagguuucg     1200

gaagccucgg ucuagacugc gagccccgca ccgggcuaga cuucagugac cuauacuacc     1260

ugacaaugaa caacaaacac uggcuagucc acaaagaaug guuccaugac aucccacuac     1320

cguggcacgc aggcgcagau accggcaccc cacacuggaa uaacaaagaa gcccucgucg     1380

aguucaaaga cgcgcacgcc aaacgccaaa cuguggucgu acucgggagu caggaaggcg     1440

caguacacac cgcacucgca ggcgcccuag aagcagaaau ggacggcgca aaaggacgcc     1500

uauccagcgg acaccucaag uguagacuaa aaauggacaa acuccgacuc aaagguguaa     1560

gcuacucccu augcacggca gcauucaccu ucacgaaaau accagcggaa acacuccacg     1620

gaacaguaac aguagaaguc caauacgcag ggacugacgg gccaugcaaa guacccgcgc     1680

aaauggcagu agacaugcaa acacucacac cggucggacg acuaauaaca gcaaacccgg     1740

uaaucaccga aagcaccgaa aacagcaaaa ugaugcucga acuagaccca cccuucggag     1800

acucauacau cgucaucggc guaggagaaa agaaaaucac gcaccacugg cacagaucag     1860

gaucgacuau aggcaaagcc uucgaagcaa cagucagagg ggccaaacgc auggcaguac     1920

ugggggacac agccugggac uucggguccg ucggcggggc ucuaaacagc cuaggcaaag     1980

ggauacacca aaucuucggc gcagcauuca aaucacucuu cggcggcaug agcugguuca     2040

gccaaauccu aaucggaaca cuacucaugu ggcuaggguu aaacaccaaa aacggcagca     2100

ucagccucau gugcuuagcc cuaggcgggg uacucauauu ucuaagcaca gcaguaagcg     2160

ccgacguagg augcagcgua gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7161
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  7161
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacgu       60

ccguaggcau cguggggcuc cugcuaacga cggccauggc agcagaggua acccgccgag      120

gcucagcaua cuacauguac cuagaccgaa acgacgcggg agaagccauc agcuucccca      180

ccacccucgg aaugaacaaa ugcuacaucc aaauaaugga ccucggccac augugcgacg      240

cgacaauguc auacgaaugc ccaauguuag acgaaggggu cgaacccgac gacgucgacu      300

gcuggugcaa caccacaagc acauggguag uauacgguac cugccaccac aagaaaggcg      360

aagccagacg cagccgcaga gccguaaccc ucccguccca cagcacacgg aaacuccaga      420

cacggaguca aacauggcug gaaucgcgcg aauacaccaa acaccuuaua agaguagaaa      480

acuggaucuu ccgaaacccg ggcuucgcgc ucgccgcagc cgccaucgca uggcuccugg      540

gguccagcac cagccaaaaa gucauauacc ucgucaugau ccuccucaua gcccccgccu      600

acuccauccg cugcaucggu guguccaacc gagacuucgu agaaggaaug ucgggaggaa      660

cauggguaga cauaguccuc gaacacggcg ggugcgucac cgucauggcg caagacaaac      720

caacaguaga cauagagcua guaacaacaa cagucagcaa cauggcugaa guccgauccu      780

acugcuacga agcaucaauc agcgacaugg caucugacag ucgaugcccc acacaaggug      840

aagccuaccu cgacaaacag ucagacacac aauacguaug caaacgcaca cucguagaca      900

gaggcugggg caacggaugc ggacuguucg gcaaaggcuc acugguaaca ugcgccaaau      960

ucgcaugcag caagaaaaug acaggcaaaa gcauccaacc agaaaaccua gaauaccgca     1020

ucaugcuaag cguccacgga agccaacaca gcggcaugau agucaacgac accgggcacg     1080

aaacagacga aaaccgagcc aaagucgaaa ucacgccaaa cagcccccgc gccgaagcaa     1140

cacuaggcgg cuucggaagc cucggccuag acugcgaacc acgcacagga cuagacuuca     1200

gcgaccuaua cuaccucacu augaacaaca aacacuggcu aguacacaaa gaaugguucc     1260

acgacauacc ccucccuugg cacgccggcg ccgacaccgg uacaccccac uggaacaaca     1320

aagaagcccu cguagaguuc aaggacgcuc acgccaaacg acaaaccguc gucguucucg     1380

gaucccaaga aggcgcagua cacacagcac uagccggcgc ccuagaagcu gaaauggacg     1440

gagccaaagg acgccuauca ucaggacauu uaaagugccg ccucaaaaug gacaaacucc     1500

gacucaaagg agucuccuac agccugugca cagcagccuu cacauucaca aaaauacccg     1560

cagagacccu gcacggaacc guaaccguag agguccaaua ugccggaacc gacggccccu     1620

gcaaaguccc agcccaaaug gcagucgaca ugcaaacacu cacccccgua ggaagacuaa     1680

ucaccgcaaa cccaguaauc accgaaagua ccgaaaacag caaaaugaug cuagaacucg     1740

aucccccauu cggcgacagc uacauaguca ucggggucgg cgaaaagaaa aucacccacc     1800

acuggcaccg cucaggauca acaauaggaa aagcauucga ggcaaccgua cgcggcgcca     1860

aacgcauggc cguccuagga gacacagcau gggacuuugg cuccguagga ggcgcgcuca     1920

acagccucgg aaaaggaaua caccaaaucu ucggggccgc cuucaaaucc cuauuuggag     1980

gaaugucgug guucucgcaa auccucauag gcacacuccu gauguggcuc gggcuaaaca     2040

ccaaaaacgg cuccaucagu cuaauguguc ucgcccucgg aggaguccua aucuuccuaa     2100

gcacagcugu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7162
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 2 opt

<400>  7162
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucggaau uguaggacug cuccuaacga cagcaauggc agccgaagua accagaagag      120

gcuccgcaua uuauauguac cucgaccgga acgacgccgg agaagccauc uccuucccaa      180

ccaccuuagg uaugaacaaa ugcuacaucc aaaucaugga ccucggacac acgugcgacg      240

caaccauguc cuacgaaugc ccaaugcuag acgaaggcgu agaaccagac gacguagacu      300

gcuggugcaa caccaccucg acuuggguag uauacggaac cugccaccac aaaaagggcg      360

aagcacgccg aucacgcaga gccgucaccc uccccucaca cagcacccga aaacugcaaa      420

cacgguccca aaccuggcuc gaaagccgag aauauacuaa acaccucaua agaguagaga      480

acuggauuuu cagaaacccg ggcuucgccc ucgccgcggc ggcaauagca uggcuccuag      540

gcuccuccac aucgcaaaaa gugauauauc ucgucaugau acuacucauc gccccagcau      600

acuccauacg augcaucggc guauccaaua gagacuucgu cgaaggaaug agcggaggca      660

cgugggugga cguaguccuc gaacacggcg gcugcguaac cgucauggca caagauaaac      720

caacgguaga caucgaacuc gucacaacca ccguaucaaa cauggcugaa guccgauccu      780

acugcuacga agccuccauc agcgacaugg cauccgacuc acgcugcccc acccaaggcg      840

aggccuaccu ggacaaacaa ucagacaccc aauacgucug uaagcgcacc cugguggacc      900

ggggcugggg caacgggugc ggacucuucg gcaaaggcag ccucguaaca ugcgcaaaau      960

ucgccugcag caagaaaaug acaggaaaaa gcauccaacc cgaaaaccuc gaauaccgaa     1020

uaaugcuaag cguacacgga ucccaacacu ccgguaugau aguaaacgac acaggccacg     1080

agaccgacga aaaccgcgcc aaagucgaaa uuacccccaa uucgccccga gcagaagcua     1140

cccuaggagg guuuggcucc cuaggacugg acugcgaacc acgcaccgga cuagacuucu     1200

ccgaccuaua cuaccucacc augaacaaca aacauuggcu aguacauaag gaaugguucc     1260

acgacauccc acuacccugg cacgccggag ccgacacagg cacaccccac uggaacaaca     1320

aagaggcacu cguagaguuc aaagacgccc acgccaaaag acaaaccguc gucguucucg     1380

gcucacaaga aggcgccgua cacacggccc ucgcuggugc ccuggaagcc gaaauggacg     1440

gagccaaagg acgccucucc agcggccacc uaaaaugccg ccuaaaaaug gacaaacucc     1500

gccucaaagg cguauccuac ucacucugca ccgccgccuu caccuucacc aaaauacccg     1560

cagaaacgcu ccacggcaca guaaccgucg aaguacagua cgcuggaacg gauggucccu     1620

gcaaaguacc cgcccaaaug gcaguagaca ugcaaacccu cacccccgua ggccgccuca     1680

ucaccgccaa cccaguaauc acagaaucaa ccgaaaacuc gaaaaugaug uuagaacucg     1740

acccacccuu cggagacuca uacaucguca ucggcgucgg cgagaaaaag aucacgcacc     1800

acuggcaccg cagcggcuca acaaucggaa aggccuucga agcaaccgua agaggagcca     1860

aacgcauggc cguacucggc gacaccgcau gggacuucgg cuccgucggc ggagcacuaa     1920

acucccucgg caaaggaauc caccaaaucu uuggcgcagc uuuuaaaucc cucuucggcg     1980

gcaugucaug guucucucaa auccucaucg gcacacuccu aauguggcuc ggguuaaacg     2040

cuaaaaacgg cuccaucagc cucaugugcc uagcacucgg cggaguacua auauuccuau     2100

caaccgcagu aucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7163
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 2 opt

<400>  7163
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

ccauaggcau cauaggauua cuacuaacca ccgccauggc cgccgaaaua acacgccggg      120

gauccgcuua cuauauguac cucgaccgca gugacgccgg caaagcuauc agcuucgcaa      180

ccacucuagg aguaaauaaa ugccacguuc aaaucaugga ccuaggccac augugcgacg      240

ccacaauguc cuacgaaugc cccaugcuag acgaaggugu agagccggac gacgucgacu      300

gcuggugcaa uaccaccagc accugggucg ucuacggcac gugccaccac aagaaaggag      360

aagcacgucg uucccgacgc gccguuacuc ugccaagcca cuccacccgu aaacuccaga      420

ccagaucaca aacauggcua gaguccagag aauacaccaa acaccucauc aaagucgaga      480

acuggauauu ccgaaacccc ggcuucgccc ucgucgccgu cgcaaucgcc uggcuacucg      540

gauccagcac uucacagaaa gucaucuacc ucgucaugau ccuacucauc gccccggcuu      600

acagcauccg augcaucgga gugagcaacc gugacuucgu cgaaggcaug ucuggcggua      660

ccuggguaga cgucguccua gaacacggag gcugcgucac cguaauggcc caagacaaac      720

ccacugucga cauagaacuc gucacaacaa ccgucagcaa uauggcagaa guccgcuccu      780

acugcuacga ggccuccaua agcgacaugg caucagacuc ccgcugccca acccaaggag      840

aagccuaccu ggacaaacaa uccgacacgc aauacgucug caaaagaacc uuagucgacc      900

gcggaugggg aaacgggugc ggccuauucg gcaaaggcuc gcucguaacc ugcgccaagu      960

ucacaugcag caaaaagaug acgggcaaaa gcauacaacc ggaaaaccuc gaauacagaa     1020

uaaugcucuc gguccacggc ucccaacaca gcggaaugau uggauacgaa acugacgagg     1080

acagagccaa agucgaggua accccaaacu ccccgcgcgc ugaagccacc cuaggaggcu     1140

ucggaagccu gggccuugac ugcgaacccc gaacaggacu ggacuucuca gaccuguacu     1200

acuugaccau gaacaacaaa cacuggcugg uccauaaaga augguuccac gacauccccc     1260

uuccauggca ugcaggcgca gacaccggca caccccacug gaacaacaaa gaagcccucg     1320

ucgaguucaa ggacgcacac gcaaaacguc aaaccguugu cguccuaggc ucacaagaag     1380

gagcaguuca caccgcccuc gcgggcgccc uagaggcaga aauggacgga gccaaagggc     1440

gccucuucag cggccaccua aaaugccgcc uaaaaaugga caaacuccgc cugaaaggag     1500

uaucguacuc ccucugcacc gcagcauuca ccuucaccaa aguacccgcg gagacccuac     1560

acggaacagu aaccguugag guccaauaug ccggaaccga cggcccaugc aagauccccg     1620

uacagauggc cguagacaug caaacacuga caccagucgg acgccuaauc accgcaaacc     1680

ccgugaucac agaguccacc gaaaacagca aaaugaugcu cgagcucgac ccaccuuucg     1740

gcgacagcua caucgucaua ggagucggag acaagaaaau aacccaccac uggcaccgaa     1800

gcggguccac gaucggcaaa gccuucgaag cgacaguuag aggagccaaa cguauggccg     1860

uacuuggcga caccgccugg gacuucggaa gugucggcgg cgucuucaac ucacucggca     1920

aaggaaucca ccaaaucuuc ggagccgcgu ucaaaucacu auucggagga auguccuggu     1980

ucucccaaau ccuuauagga acacuccugg uauggcuagg ucuaaacacc aaaaacggca     2040

gcauaucccu aaccugccuc gcgcucggcg gugucaugau auuccucucu accgccgucu     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  7164
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 2 opt

<400>  7164
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

guguaggcau cgucgggcuc cuacucacca cggccauggc ggcagagguc acccgacgug      120

gauccgcaua uuacauguac cucgaccgaa acgacgccgg ggaagcgaua uccuucccga      180

ccacccucgg aaugaacaaa ugcuacauac aaaucaugga ccuaggacac augugcgacg      240

caacaaugag cuacgaaugc ccaaugcucg acgaaggcgu ggaaccagac gacgucgacu      300

gcuggugcaa caccaccucc acauggguag uauacggcac augccaucac aagaaaggcg      360

aagcccgacg guccagacgc gcugugacac ucccaagcca cagcacaaga aaacuccaaa      420

cacgcuccca gaccuggcuc gaaagccggg aauauacaaa acaccucaua cgcgucgaaa      480

acuggauauu ccgcaacccg ggguucgcac ucgcggccgc cgccaucgca uggcuccuug      540

gcagcagcac uucacaaaaa gugauauacc ucguaaugau ccuccugauc gcgcccgccu      600

acuccauccg cugcauaggc gucagcaacc gcgacuucgu ggaaggaaug uccggcggca      660

cgugggucga cgucguacuc gaacacggag gcugcgucac aguaauggcc caagacaaac      720

caacggucga caucgaacuu guaaccacca caguaagcaa cauggccgaa guccgcucau      780

acugcuacga ggcaagcaua uccgacaugg caucagauuc acgcugcccc acccaaggag      840

aagccuaccu ggacaaacaa ucagacacac aauacguaug caaacggacc cucguagacc      900

gcggcugggg caacggcugc ggacucuuug gcaaagguuc gcucguaacc ugcgccaaau      960

ucgccugcuc caaaaagaug accggaaagu caauccaacc cgagaaccuc gaauaccgaa     1020

ucaugcucuc gguccacggg ucccaacaca guggcaugau cgucaacgac acgggacacg     1080

aaacagacga gaacagagcc aaaguagaaa ucacccccaa cuccccaaga gcggaagcaa     1140

cacucggcgg cuucggcagc cuuggccuag acugcgaacc acgaaccgga cuggacuuca     1200

gugaccucua uuaccuaaca augaacaaua aacacuggcu cguacacaaa gaaugguucc     1260

acgacauacc gcuacccugg cacgcgggug ccgacaccgg aaccccacac uggaauaaca     1320

aggaagcccu agucgaauuu aaagacgcac acgccaagag acaaacaguc guaguacuag     1380

gcucacaaga aggcgcagua cacacagccc uagcaggagc gcucgaagcc gaaauggacg     1440

gcgcgaaagg ccgacugagc agcggccacc uaaaaugccg acuaaaaaug gacaaacuac     1500

gccuuaaagg agugucauac agccucugca cagcggcauu caccuucacc aagauaccug     1560

cagaaacacu ucacggcacc guaaccgucg aaguacaaua ugccggaaca gacggcccau     1620

gcaagguacc cgcgcaaaug gccgucgaca ugcaaacccu aacccccgua ggacgacuaa     1680

ucacagcaaa ccccgucauc accgaaucga cagaaaacuc aaaaaugaug cuagaacuag     1740

aucccccauu cggcgacuca uacauaguca uaggcgucgg cgaaaagaaa aucacacacc     1800

auuggcaccg auccggaagc acaauaggaa aagcauucga ggcaacagua agaggcgcca     1860

aacgcauggc aguccucggc gacacagcau gggacuucgg cagcguagga ggagcacuaa     1920

acucccuggg aaaaggaaua caccaaauau ucggcgccgc auucaaaagc cucuucggag     1980

ggaugagcug guucucccaa auccuaaucg ggacccuccu gauguggcua ggccuaaaca     2040

ccaaaaacgg gagcaucagc cucaugugcc uagcacuagg cggcguccua auauuccuau     2100

caaccgcagu uagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7165
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 2 opt

<400>  7165
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaagucau auaccugguc augauccuac ucaucgcccc agccuacagc auacgcugca      120

uaggaguauc gaaccgagac uuugucgagg gcaugagcgg aggcacaugg gucgacaucg      180

uccucgaaca cggcgggugc gugacaguaa uggcacaaga caaaccgaca guggacauag      240

agcuagucac aaccacgguc ucgaacaugg cagaaguaag gagcuacugc uacgaagcca      300

gcauaagcga cauggcauca gacucaaggu gcccaacaca aggcgaggcu uaccucgaca      360

aacaaagcga cacccaauac gugugcaaac gcacccuagu agaccgcggu uggggcaacg      420

gaugcggccu guucggcaaa gggagccuag ucacaugcgc gaaauucgcc ugcagcaaga      480

aaaugacugg aaaaagcauc cagccggaaa aucucgaaua ccgcauaaug cucagcgugc      540

acgggagcca acacucagga augaucguga acgacaccgg acacgaaaca gacgaaaaca      600

gagcgaaagu agaaauaacc ccaaacucac caagagccga agccacacua ggaggauucg      660

gcucgcuagg ccuugacugc gaaccccgaa ccgggcuaga cuucucggac cucuauuacc      720

ucaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auaccgcuac      780

ccuggcacgc aggcgcagac acaggcacac cccacuggaa caacaaagag gcccucguag      840

aauuuaaaga cgcccacgca aaacgccaaa caguggucgu acuagggagc caagaagggg      900

caguucacac ggcccuagcc ggagccuuag aggcggaaau ggacggagcg aaaggacgcc      960

ucucgagcgg ccaucuaaaa ugucgccuga aaauggacaa acucaggcuc aaaggcguau     1020

ccuacagccu auguaccgcc gcguucacau ucacaaaaau acccgcagaa acgcuacacg     1080

gcacaguaac agucgaggua caauacgcag gcacagacgg gcccugcaaa guacccgcgc     1140

aaauggcggu agacaugcaa acccuaacac ccgucggcag acuaauaacc gcaaacccag     1200

uaaucacaga gagcacagag aacagcaaaa ugaugcucga gcucgaccca ccguucggag     1260

acucauacau agucauaggc gugggcgaaa agaaaauaac gcaccacugg caccgcuccg     1320

gaucaaccau aggcaaagcc uucgaagcca ccguacgagg cgccaaaaga auggccgugc     1380

ucggagacac agccugggac uucggcucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7166
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 2 opt

<400>  7166
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

aaaaagucau auaccuagua augauacuac ucauagcccc agcauacagc auccgcugca      120

ucggcguauc aaacagggac uucguagaag gcaugagcgg aggaacaugg guagacguag      180

uccuagaaca cggcggaugc gugaccguaa uggcacaaga caaaccgacc guagacauag      240

agcuagucac cacaaccguc uccaacaugg cagaagucag aagcuacugc uacgaagcaa      300

gcaucagcga cauggccagc gacagccggu gcccgaccca aggggaagcc uaccuagaca      360

aacagagcga cacacaauac gugugcaaac gcacacucgu agaccgcgga uggggcaacg      420

gcugcggacu guucggcaaa ggaagucuag ucacgugcgc aaaauucgcc ugcagcaaga      480

aaaugaccgg caaaucgauc caacccgaaa accucgaaua ccgcaucaug cuaagcgugc      540

acggcaguca gcacucgggu augaucguga acgacacagg acacgaaaca gacgaaaauc      600

gcgcaaaagu ggaaauaacc ccaaacuccc ccagagccga ggccacccuc ggaggcuucg      660

gcucgcucgg acucgacugc gaaccccgga ccggccucga cuucuccgac cuguacuauc      720

uaacgaugaa caauaaacac uggcucguac acaaagaaug guuccacgac auacccuuac      780

ccuggcacgc cggcgcagac acaggaacgc cacacuggaa caacaaagaa gcccucgugg      840

aguucaaaga cgcacacgcc aagcgccaaa ccgucguagu acuaggaucc caagaaggcg      900

ccguacacac ggcccuagcc ggagcccugg aagcggaaau ggacggagca aaaggcagac      960

ucagcagcgg acaccugaaa ugccgacuga agauggacaa acuaagacuc aaaggcguga     1020

gcuacagccu cugcacagcc gcauucacgu ucacaaagau acccgcagaa acacuacacg     1080

gcacaguaac agucgaaguc caauacgccg gcacggacgg gcccugcaaa guacccgcac     1140

aaauggcagu ggacaugcaa acgcucacac ccgugggaag acuaauaaca gcaaacccag     1200

ucauaacaga aagcacagaa aacagcaaaa ugaugcuaga acucgaccca cccuucggcg     1260

acucguacau agugauaggc guaggagaaa agaaaauuac acaucacugg caccgcucag     1320

gcagcacaau aggcaaagcc uucgaagcaa cgguccgcgg agcaaaacgc auggccgugc     1380

ucggagacac cgccugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7167
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 2 opt

<400>  7167
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguaau cuaccuaguc augauccuac uaaucgcccc cgccuacucc auacgaugca      120

ucggaguaag caacagagac uucgucgaag gaaugagcgg cggaaccugg gucgacguag      180

uccuggaaca uggcggcugc gucaccguca uggcccaaga caaacccacc guagacauag      240

aacucgucac aacaacagua uccaacaugg cugaaguccg aagcuacugc uacgaagccu      300

caauaagcga cauggccuca gacagcagau gccccaccca aggagaagca uaccuagaca      360

aacaaagcga uacacaauac guauguaaac gcacacuggu cgaccgcgga uggggaaacg      420

gcugcggccu cuucggcaaa ggcagucucg uaaccugcgc caaauucaca ugcagcaaga      480

aaaugaccgg aaaauccaua caaccugaaa accucgaaua uagaaucaug cuaucggucc      540

acggaagcca acauucgggc augaucggcu acgaaaccga cgaagaccgc gccaaaguag      600

aagucacacc gaacaguccc cgagcagaag ccacccuagg cggcuucgga ucccucggcc      660

uagacugcga acccagaacc ggauuagacu ucagcgacuu auacuaccuc accaugaaca      720

acaaacacug gcucguccac aaagaauggu uccacgacau accccuacca uggcacgccg      780

gcgcagacac cggcaccccg cacuggaaca acaaagaagc ccuagucgag uucaaagacg      840

cacacgccaa acgccaaacc guagucguac ucggaagcca agaaggcgca guacacaccg      900

cccuggccgg agcccucgaa gccgaaaugg acggcgccaa agggagacuc uucagcggac      960

accuaaaaug cagacucaaa auggacaaac uccgccuaaa aggcgugucc uacucccuau     1020

gcacagcagc cuucacguuc acaaaagugc cagccgaaac acuacacggc acaguaaccg     1080

ucgaagucca auacgcggga acagacggcc caugcaaaau cccaguccaa auggcagucg     1140

acaugcaaac acucaccccc gucggacgac uaaucaccgc caacccaguc aucaccgaau     1200

ccacagaaaa cucaaaaaug augcucgaac ucgacccacc cuucggagac uccuacaucg     1260

ucauaggagu cggcgacaaa aagaucaccc accacuggca cagaagcggc agcaccauag     1320

gcaaagccuu cgaagcaaca guacgcggag caaaacgcau ggccguccua ggcgacaccg     1380

ccugggacuu cggguccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  7168
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 2 opt

<400>  7168
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auaccucgua augauccuac ucauagcccc ggccuacagc auccgcugca      120

ucggaguauc aaacagagac uucguagaag gcaugagcgg gggaacaugg guagacguag      180

uccucgaaca cggaggaugc guaacaguaa uggcacaaga caaaccgacc guagacauag      240

aauuagucac aaccacaguc ucaaacaugg ccgaaguccg cagcuacugc uacgaagcga      300

gcauaagcga cauggcaagc gauucaagau gccccaccca gggggaagcc uaccuagaca      360

aacaaagcga cacccaguac guaugcaaac gaacccucgu ggaccgcgga uggggaaacg      420

guugcggccu auucgggaaa gggagccuag ucacaugcgc aaaauucgca ugcagcaaga      480

aaaugaccgg gaaaagcaua caaccagaaa accuagaaua ccgcaucaug cugagcguac      540

acgggagcca acacucagga augaucguca acgacaccgg acacgaaaca gacgaaaacc      600

gagccaaagu agaaauaacc ccuaacucac cacgagccga agcgacacug ggcggauucg      660

gcucgcucgg acuugacugc gaaccccgga ccggacuaga cuucucggau cuauacuacc      720

ucacgaugaa caacaaacac uggcucguac acaaagaaug guuccacgac auaccccuac      780

ccuggcacgc gggcgccgac acaggaacac cccacuggaa caacaaagaa gcacucguag      840

aguucaaaga cgcacacgcc aaacgccaaa ccguagucgu acuaggaagc caagaaggcg      900

ccguccacac ggcacuagcc ggagccuuag aagccgaaau ggacggcgcc aaaggccggc      960

uaucaagugg gcaccugaaa ugcagacuga aaauggacaa acucaggcuc aaaggaguga     1020

gcuacagccu gugcacggca gcauucacau ucacaaaaau accagcggaa acacuccacg     1080

ggaccgucac ggucgaaguc caauacgcag gcacagacgg acccugcaaa guacccgcgc     1140

aaauggccgu cgacaugcaa acgcucacac caguaggcag gcucauaaca gcaaacccag     1200

uaauuaccga aagcacagaa aacagcaaaa ugaugcuaga acucgaccca ccauucggag     1260

acucauacau agucaucggc guaggagaga agaaaauaac acaccacugg caccgaucag     1320

gcuccacaau cggcaaagcc uucgaggcaa ccgucagagg agccaaacgc auggcagugc     1380

ucggagacac ggccugggac uucggcucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7169
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       2 opt

<400>  7169
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggcau agucggcuua cugcucacga ccgccauggc agcggaagua accagacgag      120

gaagcgcaua cuauauguau cuggaccgaa acgacgccgg cgaagcaauc agcuucccca      180

caacgcucgg caugaacaaa ugcuacauac agaucaugga ccucgggcac augugcgacg      240

ccacgaugag uuacgagugc cccaugcucg acgagggagu cgaaccagac gacgucgacu      300

gcuggugcaa caccaccagc accugggugg uauacggcac augccaccac aagaaaggag      360

aagcacgccg cucucgacga gccguaacac uccccuccca cagcaccaga aaacuccaga      420

caagaucaca aacauggcua gaaucacgcg aauacacaaa acaccucaua agggucgaaa      480

acuggauauu cagaaauccc ggauucgccc ucgccgccgc cgcgaucgca ugguuacucg      540

gcuccuccac cagccaaaaa gucauauacc uagugaugau acuccucauc gcuccagcau      600

acuccauccg gugcauagga guauccaacc gagauuuugu ggagggaaug ucgggaggca      660

ccugggucga caucguccuc gagcacggag gcugcguaac agucauggca caagacaaac      720

ccacagucga cauagagcua guuaccacaa ccguauccaa cauggccgaa guaagaucgu      780

acuguuacga agcaagcauc agcgacaugg ccagcgacag ccgcugcccu acccaaggcg      840

aagccuaccu cgacaaacag uccgacacac aauacguaug uaaacgcacc cuagucgaca      900

gagguugggg uaacggcugc ggacucagcg gcaaaggcag ccucgugacc ugcgcgaaau      960

ucgccugcuc aaagaaaaug accggcaaau ccauacaacc cgaaaaccua gaauaccgca     1020

ucaugcucuc gguccacgga ucacagcacu caggcaugau agucaaugac acaggacacg     1080

aaaccgacga aaaccgagcu aaagucgaaa ucaccccaaa cagcccacgc gccgaagcca     1140

cgcucggagg cuucggauca cuagggcuag acugcgaacc ccgcacagga cuagacuuca     1200

gcgaccucua cuaccugaca augaacaaca aacacuggcu cguucacaaa gaaugguucc     1260

acgauauccc ccuacccugg cacgcaggag ccgacacagg aaccccacac uggaacaaca     1320

aggaagcccu cguagaguuc aaagacgcac acgccaagcg acaaaccguc guaguauuag     1380

gcucgcaaga aggcgcagua cacacagccc ucgccggcgc acucgaagca gaaauggacg     1440

gcgccaaagg gcgacuaagu agcggccauc ucaaaugccg acucaaaaug gacaaacucc     1500

gacuaaaagg cgucucauac ucccuaugca ccgcagcauu caccuucacc aaaaucccgg     1560

ccgaaacacu ccacggaacc gucaccgucg aaguacaaua cgcaggaacg gauggcccgu     1620

guaaaguucc cgcgcaaaug gccgucgaca ugcaaacgcu uacaccaguc ggcagacuca     1680

uaacagccaa cccaguaaua accgaaagca cagaaaauag caagaugaug cucgaacuag     1740

acccacccuu cggagacucc uacauaguaa ucggcguagg agaaaagaaa aucacccacc     1800

acuggcaccg aagcggaagc acgaucggca aagcauuuga agccaccgug cgcggcgcca     1860

agagaauggc cguacucggc gacacagccu gggacuucgg uuccgucgga ggcgcacuaa     1920

acucacuggg caaaggcaua caccaaaucu uuggagcagc cuucaaaagc cucuucggag     1980

gcaugucaug guucucccag auacucaucg gcacccuacu cauguggcua ggccugaaca     2040

ccaagaacgg gucaauauca cuaaugugcc uagcccucgg cggaguacua auauuccuga     2100

gcaccgccgu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7170
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 2
       opt

<400>  7170
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gcgucggcau cgucggacug cuucucacaa ccgcgauggc agccgaagua acccgcagag      120

gauccgcaua cuacauguac cuagaccgaa acgacgccgg agaagccauc agcuucccaa      180

ccacccuggg aaugaauaaa ugcuacauac agaucaugga ccugggccau accugcgacg      240

cgaccauguc cuaugagugc ccaaugcuag acgaaggagu agagcccgau gacgucgacu      300

gcuggugcaa cacaacuucg accugggucg uguacggaac cugccaccac aagaaaggcg      360

aagcacggcg aucaagacga gccgucacac uaccaagcca cagcacccgc aagcuacaaa      420

cacgaagcca gacauggcua gaaucccgag aguacaccaa acaccuaauc cgaguagaga      480

acuggaucuu ccgcaaccca ggcuucgcac uugccgcggc cgcaaucgcc uggcugcucg      540

gcagcagcac aagucaaaag gucaucuacc uggucaugau acuccuaaua gcgccggccu      600

acagcauccg uugcaucggc gucuccaacc gagacuucgu ugaaggcaug ucaggaggca      660

cauggguaga cguaguacuc gagcacggag gcugcguaac cgugauggcg caggacaaac      720

caacaguaga caucgagcua gucaccacaa ccgugucgaa cauggcugaa gucaggagcu      780

acugcuauga agcaucaaua ucagacaugg caagcgacuc cagaugcccc acacaaggcg      840

aagccuaccu cgacaaacaa ucugacaccc aauacgugug caaaaggacc cuaguagacc      900

ggggcugggg caacgggugc ggccuaagcg gcaaaggcag ccuaguaacc ugcgccaaau      960

ucgcaugcuc caagaaaaug acaggcaaau ccauccaacc agaaaaccua gaauacagaa     1020

ucauguuauc aguccacgga agccaacacu caggcaugau cgucaacgac accggacacg     1080

aaacagacga aaaccgcgcg aaaguagaaa uaaccccaaa cucacccaga gcagaagcca     1140

cacugggcgg cuucggcucc cucggacugg acugcgaacc ccgaaccggg cuagacuucu     1200

cagaccuaua cuaccucaca augaacaaca aacacuggcu cguccacaaa gaaugguucc     1260

acgacauccc acuccccugg cacgcaggcg ccgacaccgg cacaccacac uggaauaaca     1320

aagaagccuu aguggaguuc aaagacgcac augccaaaag acaaaccguc gucguacuag     1380

gcagucaaga aggcgcagua cacacagcgc ucgcgggcgc gcuggaagcc gaaauggacg     1440

gagcaaaagg cagguuauca agcggccacc ucaaaugccg acuaaaaaug gauaaacuac     1500

gccucaaagg cguaagcuac agccucugca cagcagcauu caccuucacc aaaauacccg     1560

ccgaaacacu gcacggcaca guaacagucg aaguccaaua cgcaggcacc gacggaccau     1620

gcaaaguccc cgcccagaug gccgucgaca ugcaaacccu cacucccgua gggcgcuuaa     1680

ucaccgccaa cccagucauc acagaaucca cagaaaacag uaaaaugaug cucgaacucg     1740

acccgccuuu cggagacucc uacaucguaa ucggcguagg cgaaaagaag aucacccauc     1800

acuggcacag aucagguagc accaucggca aagcguucga agcaaccgug cgcggcgcga     1860

aacgcauggc aguucucggc gacacugcau gggacuuugg cucaguagga ggcgcacuca     1920

acagccuagg aaaaggaaua caccaaauau ucggcgccgc auucaaaagc cuauucggcg     1980

gaaugucaug guucagccaa auacucauug gcacccuccu cauguggcug ggccucaacg     2040

ccaaaaacgg cuccauaucg cucaugugcc ucgcacuagg cggcguauua auauuccucu     2100

caacagcugu cagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7171
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 2 
       opt

<400>  7171
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccauaggcau aaucggccua cugcuuacga cagcaauggc cgcagaaaua acacgacggg      120

gcuccgccua cuacauguac cucgaccgcu cggacgccgg aaaagcaauc aguuucgcua      180

caacccucgg cguaaacaaa ugccacgucc aaaucaugga cuuaggccac augugcgacg      240

cgacuauguc cuacgaaugc cccauguuag acgagggcgu cgaacccgac gacgucgacu      300

gcuggugcaa caccaccucg acauggguag ucuacggcac uugccaucac aagaaaggag      360

aagcacgccg uucaagacga gcagucaccc uacccagcca cagcacacga aaacuacaaa      420

cccgcuccca aaccuggcua gaaagccgcg aauacaccaa acaccucaua aaagucgaaa      480

acuggauauu ccgaaacccc ggcuucgcac ucgucgccgu agccaucgca uggcuccuag      540

gauccagcac cagccagaaa gucauauacc uagucaugau ucuacucauc gcaccggcau      600

acuccauacg cugcaucgga guaagcaaca gagacuucgu cgaagguaug uccggcggca      660

cuugggucga cgucguccuc gaacacggag gaugcgugac agucauggca caagacaaac      720

caacagugga caucgaacuc guaaccacca ccgucagcaa cauggccgaa guccgaagcu      780

acugcuacga agccagcauc agcgacaugg cuucagacag ccgcugccca acgcaagggg      840

aagccuaccu cgacaaacaa ucagacaccc aauacguaug caaacgcacc cuggucgauc      900

gcggaugggg caacgggugc ggccuuucug gcaagggguc ccuaguaacc ugcgcaaaau      960

ucaccuguuc aaagaaaaug accggcaaau ccauccaacc ugaaaaccuc gaauaccgua     1020

uaaugcucag uguccacgga ucccaacaca gcggaaugau cggcuacgaa acagacgaag     1080

accgcgccaa aguagaagua acccccaacu cgccacgagc cgaggccaca cuaggcgguu     1140

ucggcucccu aggacucgac ugcgaaccuc gcacagggcu cgacuucucc gaccuauacu     1200

accugacaau gaacaacaaa cacugguuag uccauaagga augguuccau gacauacccc     1260

uccccuggca cgcuggcgcc gacacaggca caccgcacug gaacaacaaa gaagcucuag     1320

uagaauuuaa agacgcccac gccaaacgac aaacagucgu uguccuaggc ucccaagagg     1380

gagcaguaca cacagcccua gcgggcgccc uagaagccga aauggacggc gccaaaggcc     1440

gccucuucuc cggccaccuc aaaugucgcc ucaagaugga caaacuccgc cuaaaaggcg     1500

uaucuuauag ccuaugcaca gcagcauuca cauucacaaa aguaccagcc gaaacgcucc     1560

acggaacggu cacagucgaa guccaauacg ccggcaccga cggaccgugc aagaucccag     1620

uacaaauggc aguagacaug cagacccuaa caccagucgg ccgccugaua acugccaacc     1680

cagucauaac cgaaucgacc gaaaacucaa agaugaugcu ugagcuugac ccacccuucg     1740

gcgacuccua cauuguaauc ggcgucgggg acaagaaaau cacccaccac uggcacagaa     1800

gcggauccac cauaggaaaa gccuucgaag caaccguaag aggagccaaa agaauggcag     1860

uccucggcga uacagcuugg gacuucggaa gcgucggagg cgucuucaac ucccucggua     1920

aaggcaucca ccaaauauuc ggcgcagcau ucaagucccu guucggcgga augucauggu     1980

ucucacagau acuaaucgga acacugcuag uauggcuagg ccucaacacc aaaaacggca     2040

gcaucagccu uacgugccua gcacugggcg gagucaugau cuuucucucc accgccguau     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  7172
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 2 
       opt

<400>  7172
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguagggau aguaggacuc cuccugacaa cagccauggc cgccgaaguc acaagacgug      120

gcagcgcgua cuacauguac cuggauagaa acgacgcagg cgaagcaauc aguuucccca      180

caacacuggg caugaacaag ugcuacaucc aaaucaugga ccugggccac augugcgaug      240

ccaccaugag cuacgaaugc cccaugcuag acgaaggcgu cgaacccgac gacgucgacu      300

gcuggugcaa cacaacaagu acgugggucg uguacggcac augucaccac aaaaagggag      360

aagcucggcg gucccgccga gccgucacgc uccccagcca cagcacaaga aaacuccaaa      420

cacgaagcca aacauggcua gaaucaagag aauacaccaa acaccucaua agaguggaaa      480

acuggaucuu cagaaaccca ggcuucgcac uagccgcagc cgcaaucgcg ugguuacuug      540

gcuccagcac uucucagaaa gucauauacc ugguaaugau acuacuaaua gccccggcau      600

acucgauacg augcaucggg guaaguaaca gggacuucgu cgaaggaaug uccggaggca      660

cauggguaga cguaguccua gaacacggcg gaugcguuac gguaauggcc caagacaaac      720

caaccguaga caucgaacug gucacaacaa ccguaagcaa cauggcagag gucagaagcu      780

acugcuacga agccucaaua agcgacaugg caagcgauuc ccggugccca acucaagggg      840

aagcauaccu agacaaacaa uccgacacac aauacguaug caaacgcaca cuuguagaca      900

gaggcugggg gaacggcugc ggacuauccg gaaaaggcuc guuagugaca ugugcaaaau      960

ucgcaugcag caagaaaaug acagggaaau caauacaacc cgaaaaccua gaauaucgga     1020

ucaugcucuc cguacacggc ucacaacaca gcggaaugau cguaaacgac accggacacg     1080

aaacagauga aaaccgagcu aaaguagaaa ucacccccaa cagcccaaga gcagaagcaa     1140

cacucggagg auucgguuca cuuggacucg acugcgagcc cagaaccggc cucgacuucu     1200

cagaccuaua cuaccuaacg augaauaaca aacacuggcu cguacacaaa gaaugguucc     1260

acgacauacc ccugccgugg cacgcagggg cggacacagg aaccccacac uggaacaaca     1320

aagaagcgcu ggucgaguuc aaagacgccc acgcuaaacg ccaaaccgua guaguacucg     1380

gaagccagga aggagccgua cacaccgccc uggccggggc acucgaagca gaaauggacg     1440

gcgccaaagg ccgccuaagc agcggacacc uaaaaugccg ucugaaaaug gacaaauugc     1500

gacucaaggg aguauccuac ucccuuugca ccgcagcauu cacauucaca aaaauacccg     1560

ccgaaacacu acauggcaca guaacaguag agguccaaua cgcaggcacg gacgguccau     1620

gcaaaguacc cgcgcaaaug gccgucgaca ugcaaacacu gacuccaguc ggcagacuca     1680

uaaccgcaaa ccccguaaua accgaaagua ccgaaaacag caaaaugaug uuagagcuag     1740

acccacccuu cggcgacagc uacauaguaa uaggagucgg cgagaaaaag auaacccacc     1800

acuggcaccg cagcggaagc accaucggca aagccuucga agccacaguc cgcggggcca     1860

aacgaauggc aguccugggc gacacagcau gggacuucgg gucagucggc ggcgcacuaa     1920

acagccucgg gaaaggcaua caccaaaucu ucggugcagc uuucaaaagc cuauucggug     1980

ggaugucaug guucagccaa auacucauag gcacccuccu cauguggcug ggccucaaca     2040

ccaaaaacgg cucaauaagc cuaaugugcc uagcacuagg cggcguccug auauuccugu     2100

ccacagccgu auccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7173
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       2 opt

<400>  7173
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucggaau agucggacuc cuccucacaa cagcaauggc cgcggaaguc acccgacgcg      120

gcagcgcuua cuacauguac cucgaccgaa acgacgccgg cgaagccauc agcuucccca      180

ccacccuggg caugaacaaa ugcuacauac aaauaaugga ccuuggccac augugcgacg      240

cgaccaugag cuacgaaugc cccaugcucg acgaaggagu ggagccagac gauguagacu      300

guuggugcaa cacaacauca accugggucg ucuacggcac augccaccac aagaagggag      360

aagccagacg cagccgcaga gcaguaaccc uaccguccca cucuaccaga aaacuccaaa      420

cacgaagcca gacaugguua gaaagccgcg aauacacaaa acaccucauc agagucgaga      480

acuggauauu cagaaaccca ggcuucgcau uagccgcagc cgcaaucgcc uggcuccuag      540

ggagcucaac cucacaaaaa guaauauacc uagucaugau acuacucaua gccccggccu      600

acagcauacg augcauaggu gucucaaaca gggacuucgu cgaaggcaug agcggaggca      660

cgugggugga cauaguacuc gaacacggcg gcugcgugac cgucauggca caagacaaac      720

cgacaguaga cauagaauug gugacaacca cagucuccaa cauggccgaa gugcguucau      780

acugcuacga agcaagcauc agcgacaugg ccucagacag ccgaugccca acccaaggcg      840

aagccuaccu agacaagcaa agcgacacac aauacgucug caagcggacg cuaguggacc      900

gaggcugggg caacggcugc ggacuauucg gcaaaggaag ccucguaaca ugcgcgaaau      960

ucgcgugcag caagaaaaug accggcaaau ccauccaacc cgaaaaccuc gaauacagaa     1020

uuaugcucuc cguacacggc agccaacacu ccggcaugau uguacaagau acaggccacg     1080

agacagacga aaacagagcg aaagucgaaa uaacccccaa cucgccccgg gccgaagcaa     1140

cucuaggagg auucggaucg cuagggcuug acugcgaacc ccgaacagga cuagacuucu     1200

ccgaccugua cuaccuaaca augaacaaca aacacuggcu aguacacaaa gaaugguucc     1260

acgacauccc cuuacccugg cacgcaggcg ccgacacagg aaccccacac uggaacaaca     1320

aagaagccuu aguggaguuc aaagacgcac acgcaaaacg ccaaaccgua guaguacuag     1380

gaagccaaga aggagcagua cacacagccu uggccggagc guuagaagcc gaaauggacg     1440

gggcaaaagg caggcuuagc ucgggacacu uaaaaugcag acuaaaaaug gacaagcucc     1500

gccugaaagg aguauccuac ucccuaugca cagccgcguu cacauucacg aaaauacccg     1560

ccgaaacuuu acacggcacg gucacagucg agguccaaua cgcaggcacc gacgggccau     1620

gcaaaguacc cgcccaaaug gccgucgaua ugcaaacccu aaccccagua ggaaggcuua     1680

uuaccgccaa cccagucaua acagaaagca cagaaaacuc aaaaaugaug cuagaacuag     1740

auccacccuu cggagacucc uacaucguca uaggcguugg agaaaagaaa aucacacacc     1800

acuggcacag aucaggaagc accauaggca aagccuuuga agcgacagua cgcggcgcaa     1860

agagaauggc cguacuuggc gacacagccu gggacuucgg cucagucggg ggcgcgcuga     1920

acagccuagg caaagggaua caccaaauau ucggagcagc auucaaaucc cucuucggcg     1980

gaaugucaug guucucacaa auccucaucg gaacccuacu gauguggcua ggccuaaaca     2040

ccaaaaacgg cagcaucuca cucaugugcc uggcccuagg cggaguccuc aucuuccucu     2100

ccaccgccgu aucggccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7174
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 2
       opt

<400>  7174
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguaggaau cgucgggcua cuacucacaa cagcaauggc ggccgagguc acccgcagag      120

gauccgccua cuacauguac cuagaccgaa acgacgcagg cgaagcgauc agcuucccaa      180

cuacacuagg aaugaacaaa ugcuacauac aaauaaugga ccuuggacac acaugcgacg      240

ccaccaugag cuacgaaugc ccaaugcugg acgagggagu ugagcccgac gauguggacu      300

gcuggugcaa caccacgagc acguggguug ucuauggcac augccaccac aagaaaggcg      360

aagccagacg cagccgacgc gcaguaacac ucccaucaca cucaacaaga aaacuucaaa      420

caagaagcca aaccuggcug gaaagccgag aauacacaaa acaccugaua cgagucgaaa      480

acuggaucuu ccggaacccc ggauucgcac ucgcggcugc agccaucgcc uggcuacucg      540

gcuccagcac aucccaaaaa gucaucuacc ucgugaugau auuauugauu gcacccgcau      600

acucaaucag augcaucggc guaagcaauc gugauuucgu ggaggggaug ucaggcggca      660

ccugggugga cgucguacua gaacacgggg gaugcgucac aguuauggcc caagacaaac      720

caacagucga caucgaacug guaaccacaa ccguaagcaa uauggccgag gugcgaucau      780

acuguuacga ggccagcaua agcgacaugg cuucagacuc ccgcugcccc acccaaggag      840

aagcguaccu agacaaacaa ucagacacac aauacguaug caaaaggacc cuagucgacc      900

gaggaugggg caacggaugc gggcuauucg gcaagggcag ccuaguaacu ugcgccaaau      960

ucgcaugcuc caagaaaaug acaggcaaaa gcauacaacc agaaaaccua gaauacagaa     1020

ucaugcuauc gguacacgga agccaacaca gcgggaugau aguacaagac acgggacacg     1080

aaacagacga aaacagggcc aaaguagaga uaacgcccaa caguccccgg gcagaagcaa     1140

cacuaggcgg cuucggauca cuaggccuag acugcgaacc acgcacaggc cuagacuuca     1200

gcgaccucua cuaccucacc augaacaaca aacacuggcu gguacauaaa gaaugguucc     1260

acgacauacc guuaccgugg cacgcaggcg cagacaccgg cacaccccac uggaacaaca     1320

aagaggcccu cguagaguuu aaagacgccc acgcaaaacg gcaaacaguc guaguccuag     1380

gaucgcaaga gggcgcaguc cacacagcac uagcaggcgc ccuagaagcc gaaauggacg     1440

gagccaaagg acgacucucc uccggccacc uaaaaugcag acucaagaug gauaaacucc     1500

gacuaaaagg cgucuccuac ucgcucugca cagcggcauu uacauucacc aaaauaccag     1560

ccgaaaccuu acacggaacc guuaccgucg aaguacagua cgcaggaaca gacgggccau     1620

gcaaaguccc ggcacagaug gccgucgaca ugcaaacccu caccccaguc ggccgacuca     1680

ucacggccaa ccccgugauc acagaaucga ccgaaaauuc aaaaaugaug cuagaacuug     1740

acccacccuu cggagacagc uacaucguca uaggcgucgg cgagaagaaa aucacccauc     1800

acuggcaccg cagcggaagc accauaggaa aggccuucga ggcaaccgug agaggagcga     1860

aacgaauggc cguacucggc gacacugcau gggacuucgg cuccgucggc ggagcucuaa     1920

acucccuggg aaaaggaauc caccaaauau ucggagccgc cuucaaaagc cuauucggag     1980

gaauguccug guucucacaa auacuaauag gcacccuccu gauguggcua ggacuaaacg     2040

caaagaacgg guccauaagc cugaugugcc uggcgcucgg gggcguccua aucuuccuau     2100

ccacagcagu auccgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7175
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 2 
       opt

<400>  7175
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggaau cgucgggcuc cugcuaacga cagcaauggc agcagaaguc acucgacgag      120

gcuccgcaua cuacauguac cuggaccgaa acgacgccgg agaagccaua uccuucccaa      180

ccacacuugg caugaacaaa ugcuauaucc aaauaaugga ccucggacac augugcgaug      240

ccacaauguc auacgaaugc ccaaugcucg acgaaggcgu ggaaccagau gacgucgacu      300

gcuggugcaa caccacaagc accugggucg uauacgguac augccaccac aagaaaggcg      360

aagcacggag gagccgacga gcaguaaccc uaccaagcca cuccacucgc aaacuccaaa      420

cccgaagcca aaccuggcua gaaagccgag aauacaccaa acaccuaaua cggguagaaa      480

acuggaucuu ccgaaauccc ggcuucgcac uagccgccgc ggcaaucgca uggcucuuag      540

gcucaaguac aagccaaaaa guaaucuacc ucgucaugau ucuacuaauc gcccccgccu      600

acuccauacg cugcaucgga guaagcaaca gagacuucgu cgaagggaug ucaggcggua      660

ccugggugga cgucguacua gaacacggcg gcugcguaac cgucauggcc caagacaaac      720

caacgguaga caucgaacuc gucaccacca cagucagcaa cauggccgaa gugcgcuccu      780

auugcuauga agcaucaaua ucagacaugg cuucagacuc acgaugccca acacaaggag      840

aagccuaccu agacaaacag ucagacaccc aauacguaug caaacgaacc uuagucgauc      900

gcggaugggg aaacggcugc ggccucuucg ggaaaggcuc gcuagugaca ugcgccaaau      960

ucgcaugcuc caagaaaaug accggcaaau caauccaacc agagaaccua gaguauagga     1020

uaaugcucuc aguccacgga ucgcaacaua gcggaaugau aguccaagac acgggacacg     1080

agacggacga gaaccgcgca aaagucgaaa uaacaccaaa cagcccccga gcggaagcga     1140

cacuaggcgg guucggaagc cuaggacuag acugcgagcc acgaacgggc cuggacuucu     1200

cagaccuaua cuaccucacc augaacaaca aacauuggcu aguccacaaa gaaugguucc     1260

acgacauccc ccucccaugg cacgcgggug cagacacagg cacuccccac uggaacaaca     1320

aagaggcccu ggucgaauuu aaagacgcuc acgcgaaaag gcaaaccguc guaguacugg     1380

gcucacaaga aggcgcgguc cauacagccc ucgcaggcgc auuagaagcc gaaauggacg     1440

gagcaaaagg acgccuaucc ucaggccacc ucaaaugccg acucaaaaug gacaaacuac     1500

ggcugaaagg aguauccuac agccucugca cagccgcauu caccuucacc aaaauccccg     1560

ccgaaacccu acacgggacc guaacaguag aaguacaaua cgcagggaca gauggcccau     1620

gcaaaguccc ggcccagaug gccgucgaca ugcaaacacu aacaccgguu ggacgccuca     1680

ucacagccaa ccccgucauu acagaaucaa cagagaacuc caaaaugaug cucgaacuag     1740

acccacccuu cggagauuca uacaucguca uaggcgucgg agaaaagaaa auaacucacc     1800

acuggcaccg cagcggcagc accaucggca aagcauucga agccacaguc cgaggagcaa     1860

aaaggauggc aguacucgga gacaccgcau gggacuuugg uagcgucggc ggcgcccuaa     1920

acucacuagg aaaaggaaua caucaaaucu ucggagcagc auuuaaaagc cucuucggug     1980

gaaugagcug guucucccaa auacuaaucg gaacccuccu gauguggcua ggacugaaca     2040

ccaaaaacgg aagcaucagc cugaugugcc ucgcgcucgg gggcguacua aucuucuuaa     2100

gcacagcagu aucggccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7176
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  7176
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguuggaau cgucgggcug cuacucacga ccgccauggc cgcugaaguc acgcgcaggg      120

gcucagcaua cuacauguac cucgacagaa acgacgcggg cgaagcaauc agcuucccaa      180

caacacucgg caugaacaaa ugcuacauac aaauaaugga ccucggacac augugcgacg      240

caacaauguc guacgaaugc cccaugcucg acgaaggagu ugaacccgac gacguagacu      300

gcuggugcaa cacaacaagu accugggucg uguacggaac cugccaccac aagaaaggag      360

aagcuaggcg gucacgacgc gcaguaaccc ugccaagcca cuccacgaga aaacuacaaa      420

cccgcagcca aacauggcug gaaucacgcg aauacaccaa acaccucauc cgcguggaaa      480

acuggauuuu cagaaaccca ggcuucgcgc uagccgccgc cgccauagca uggcuacucg      540

ggucauccac aagccaaaaa gucauauacc ucgucaugau ccuguuaauc gccccagcgu      600

acagcauacg gugcaucggc gucagcaauc gagacuucgu ggaaggcaug uccggcggaa      660

ccugggucga caucguccuc gagcacggag ggugcguaac cgucauggca caagacaaac      720

caacaguaga cauagaacuc gugacaacaa caguaucuaa uauggccgaa guccgcuccu      780

acugcuacga agccagcauc ucagacaugg ccuccgacuc acgcugccca acgcaagggg      840

aagcauaccu cgacaaacaa uccgacaccc aauacguaug caaaagaaca cucguagacc      900

gagggugggg aaacggaugc ggccuauucg gaaaaggcag ccucguaacc ugcgccaaau      960

ucgcgugcuc caagaaaaug acgggcaaaa gcauccaacc cgaaaaccuc gaauacagaa     1020

ucaugcuguc cguccacggc ucccaacacu ccgggaugau cguaaacgac acgggacacg     1080

aaaccgacga aaaccgagcc aaaguagaaa ucaccccaaa cagucccaga gccgaagcca     1140

ccuugggugg uuucggaagc cucggccucg acugcgaacc gagaacaggc cuggacuuca     1200

gcgaccucua cuaccucaca augaacaaca aacacugguu aguccauaaa gaaugguucc     1260

acgacauacc ucuccccugg cacgccggcg cagacaccgg aaccccacac uggaacaaca     1320

aagaggcacu uguagaguuc aaagacgccc acgccaaacg acaaaccguc guaguccucg     1380

gaucacaaga aggcgcggua cacaccgcac ucgcgggggc ccuggaagca gaaauggacg     1440

gcgccaaggg cagacucagc uccgggcacc ucaaaugcag acugaaaaug gacaagcucc     1500

gacucaaggg agucucuuac ucacuaugca ccgccgcguu cacauucacu aaaaucccag     1560

ccgaaacacu ccacgguacc gucaccguag aaguccagua ugcaggcacu gacggcccgu     1620

gcaaagugcc cgcccaaaug gccguagaca ugcaaacacu caccccagua ggaaggcuaa     1680

ucacugcaaa ccccgugaua acagaaucaa ccgaaaacuc aaaaaugaug cuagaacuag     1740

acccacccuu cggagacucc uacaucguca uaggaguggg ggaaaagaaa aucacacacc     1800

acuggcacag guccggcucc acacucggca aagccuucuc caccacccua aaaggcgcac     1860

aacgccuagc agcccuagga gacacggcgu gggacuucgg guccauaggc ggcguauuca     1920

acuccaucgg acgcgcaguc caccaagucu ucggcggagc cuuccgaacc cuauucggcg     1980

gaaugagcug gauaacccag ggacugaugg gagcacuacu gcuauggaug ggcgucaacg     2040

cacgcgacag aagcauagca cuagccuucc ucgccacugg cggaguacuc guauuccugg     2100

caacaaacgu ccacgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7177
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  7177
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucggaau cgucggacuc cugcuaacaa cagcaauggc agccgaaguc acgcgaaggg      120

gaucggcaua cuacauguau cucgaccgca acgacgcggg cgaagccauc agcuucccaa      180

caacgcuggg caugaacaaa ugcuacauac aaauuaugga ccuaggacac acaugcgacg      240

caacaaugag cuacgaaugc cccaugcucg acgagggcgu ugaacccgac gacgucgacu      300

gcugguguaa uacgacaucc acauggguag uauacgggac cugccaccac aagaaagggg      360

aagcgcgccg guccaggagg gccgucacuc ucccgagcca caguaccagg aaacuacaaa      420

cccgcagcca aaccuggcuc gaaagccgug aauauacaaa acaccuaauu cgcguagaga      480

acuggaucuu ccgcaacccc ggauucgccc uggcagcagc agcaauagcc uggcuguugg      540

gcucaagcac aagccaaaag gucauauacc ugguaaugau ccuacuaaua gcaccagcau      600

acuccaucag augcauaggc gugagcaacc gagacuucgu ugaaggaaug ucgggcggga      660

caugggucga cguaguccua gaacacggag gcugcguaac ggucauggcu caagacaagc      720

ccacggucga cauagagcua guuacuacaa caguaagcaa cauggccgaa gucaggagcu      780

auugcuacga agccuccauc ucagacaugg cauccgacuc ccggugcccc acccaaggag      840

aagcauaccu cgacaaacaa agcgacacgc aauacgucug caaaaggacc cucgucgacc      900

gcggaugggg caacggaugc gggcuauucg gaaaaggauc ccucgugacc ugcgccaaau      960

ucgccugcuc caagaaaaug acaggcaaaa gcauacaacc cgagaaucug gaauacagaa     1020

uuaugcucag cguacacggc ucacagcaca gcggcaugau cguaaacgac accggacacg     1080

aaacggacga aaaccgagca aaagucgaaa uaacaccaaa cagcccucgc gcggaagcga     1140

cccugggagg auucgggagc cuaggacugg acugcgaacc ccgcacgggc cuagacuuca     1200

gcgaucucua uuaccuaacc augaacaaca aacacuggcu agugcacaaa gagugguucc     1260

acgacauccc acucccgugg cacgccggag ccgacacagg aaccccgcac uggaacaaca     1320

aagaggcccu uguagaguuc aaagacgcac augcgaaaag acaaacaguc guaguccugg     1380

gcucacaaga aggagccgua cacacagcac uagcaggcgc ucucgaagcc gaaauggacg     1440

gagccaaagg gcgccuaagc uccggccacc ugaaaugccg ccuaaaaaug gacaaacuac     1500

gccucaaggg aguaucauau ucccuaugca ccgcagccuu cacauucaca aaaaucccag     1560

ccgaaacacu acacggcaca guaacagucg aaguacaaua cgcaggaaca gacggcccau     1620

gcaaaguccc cgcccaaaug gcgguugaca ugcaaacccu aaccccaguc ggacgccuga     1680

ucacagcaaa cccagucauc accgaaucca cagagaacuc aaaaaugaug cucgaacuag     1740

acccgcccuu cggcgauagc uacaucguaa uagggguagg agaaaagaaa aucacacauc     1800

acuggcaccg cagcggaagc acccuaggca aagcauucuc gaccacacug aaaggagcac     1860

aacggcucgc agcccucgga gacaccgccu gggacuucgg auccauaggg ggagucuuca     1920

acucgaucgg ccgcgcagua caucaaguau ucggcggcgc guucaggaca cuauucggag     1980

gaaugagcug gaucacacaa ggccuaaugg gagcccuacu acucuggaug ggcgucaacg     2040

cacgagaccg gagcauagcc cuagcauucc ucgccaccgg aggcguacuc guauuccuag     2100

caacgaacgu acacgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7178
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 2 opt

<400>  7178
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau aaucggacuc cuccuaacga ccgcaauggc agcagaaaua acacgacgag      120

gcucagccua cuacauguac cuggaccgca gugacgcagg aaaagcgauc ucguucgcaa      180

cuacccucgg cgucaacaaa ugccacgucc aaaucaugga ccucggucac augugcgacg      240

caaccauguc cuacgaaugc ccaauguuag augaaggugu agaaccagac gacguagacu      300

gcuggugcaa cacgacguca accugggucg ucuacgguac cugccaucac aagaaaggcg      360

aagcccgccg cagcagacgc gccguaaccc uaccaagcca cucaacacga aaacuccaga      420

ccagaucaca gacguggcug gaaucccgag aauauaccaa acaccuaaua aaagucgaaa      480

acuggauauu cagaaauccc ggauucgccc ucgucgcagu agcaaucgcu uggcuacucg      540

gauccagcac aagccaaaaa guaaucuauc ucguaaugau acuccuaaua gcacccgcau      600

acuccauaag augcaucggc guaagcaacc gagacuucgu cgaaggaaug ucgggcggca      660

cauggguaga cgugguacua gagcacggag gauguguaac ggucauggca caagauaaac      720

cgaccgucga cauagaacuc guaacaacca ccguauccaa cauggccgaa gucagaagcu      780

acuguuacga agccuccauc ucagacaugg ccucagacuc ccgcugcccc acacagggcg      840

aagcauaccu agacaagcaa uccgacacac aauaugucug caaacgaacg cuagucgacc      900

gaggaugggg gaacggaugc ggacucuuug guaagggcuc acucguaacc ugcgccaaau      960

uuaccugcag caagaaaaug accggaaaaa gcauccaacc ggaaaaccua gaauacagaa     1020

uuaugcucuc gguccacggc ucacaacaca gcgggaugau aggcuaugaa accgacgaag     1080

acagagccaa aguagagguc acaccaaaca gccccagggc agaagcaacc cucggcggcu     1140

ucggaucccu cggccucgac ugugaacccc gaaccggccu ggacuucucc gaccucuacu     1200

accugaccau gaacaacaag cacugguuag uucacaaaga augguuccau gacauccccc     1260

uacccuggca cgccggcgca gacaccggca ccccacacug gaacaacaaa gaagcccuag     1320

ucgaguucaa agacgcacac gccaaacggc aaacagucgu aguccuuggc ucccaagaag     1380

gagccguaca caccgcacuc gccggagcac uagaagcaga aauggacggu gccaagggcc     1440

gacucuucag cggacaccua aaaugccgcc ucaagaugga caagcuaaga cucaaagggg     1500

ucuccuacuc ucucugcacu gcagccuuca ccuucacgaa aguccccgca gaaacccucc     1560

acgggacugu caccguagaa guacaauacg ccgguaccga cggcccaugc aaaaucccag     1620

uacaaauggc aguagacaug cagacacuua cacccguagg acgccuaaua acagcaaacc     1680

ccgucauaac cgaaagcaca gaaaacucca aaaugaugcu agaacuagac ccucccuucg     1740

gagacagcua cauagugauc ggcgucggcg acaagaaaau cacacaccac uggcacagaa     1800

gcggcagcac ccuaggcaaa gcauucucua caacccucaa aggcgcccaa cgacuagcag     1860

cacucggaga cacagccugg gacuucggaa gcaucggagg cgucuucaac agcauaggac     1920

gcgccguaca ccaagucuuc ggaggcgcuu uccgcacccu cuucggcgga augagcugga     1980

ucacccaagg gcugaugggc gcacuacuac ucuggauggg aguaaacgca cgggacagaa     2040

gcaucgcccu ggcuuuucuc gccaccggcg gcguccucgu cuuccuagca acgaacguac     2100

augccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  7179
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 2 opt

<400>  7179
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau aguaggccua cuacucacca ccgccauggc cgccgaaguc acgcgcagag      120

gaagcgcaua cuacauguac cuggaccgaa acgaugccgg cgaagcgauc uccuucccaa      180

ccacucucgg gaugaacaaa ugcuauaucc aaaucaugga ccuaggacac augugcgaug      240

caaccauguc cuacgagugc ccaaugcuag augaaggagu cgaaccagac gacguggacu      300

gcuggugcaa caccaccucg acauggguag ucuacggcac augccaccau aagaaggggg      360

aagccagacg cuccagacga gcaguaacgc uccccucaca cucaacgaga aaacuccaaa      420

ccagaagcca gacauggcuc gaaucacgcg aguacaccaa acaccucauc cgcgucgaaa      480

acuggaucuu ccgaaauccu ggcuucgccc uagcagcagc cgccauagcc uggcuacucg      540

gcagcagcac cagccaaaaa guaaucuauc uaguaaugau ccuacuaauc gcacccgccu      600

acagcauacg cuguaucggu guaucaaacc gcgacuuugu cgaaggaaug ucaggcggca      660

caugggucga cguaguccua gaacacggcg gcugcgucac aguaauggca caagacaaac      720

caacggucga cauagagcua guaacaacaa cgguauccaa cauggccgaa guccgaucau      780

acugcuacga agccuccauc ucagacaugg caucggacuc ccgcuguccg acacaaggag      840

aagcguaccu agacaaacaa ucggacaccc aauacguaug caaacgaacu cuaguagacc      900

gcggcugggg aaacggaugu ggccucuucg guaagggcuc acucgugacc ugcgcaaaau      960

ucgcaugcag caagaaaaug accggcaaau ccauccaacc ugaaaaccua gaauaccgaa     1020

uaaugcuauc aguccacggu ucacaacacu ccggaaugau cguaaacgac accggccacg     1080

aaacagacga aaaccgagcc aaaguagaaa uaacccccaa cagcccacga gcggaagcaa     1140

cccuaggggg auucggcuca cuggggcugg acugugaacc cagaacgggc cuagacuuca     1200

gcgaccucua cuaccuaaca augaacaaua aacacuggcu aguacacaaa gaaugguucc     1260

acgacauccc ccuccccugg cacgcaggcg ccgauaccgg cacuccccac uggaacaaca     1320

aagaagcucu ggucgaguuc aaagaugcac acgcuaaaag acaaaccgua gucguccuag     1380

gcucccagga aggugcgguc cacaccgccc ucgcaggagc ccucgaggca gaaauggacg     1440

gcgcuaaagg acgccuaagc uccggccacc ucaaaugccg ccuaaaaaug gacaaguuac     1500

gccucaaagg agugaguuac agccucugca cagcggccuu caccuucaca aaaaucccag     1560

cagaaacacu acacggaacc gucaccgucg agguucaaua cgcaggcacg gacgggccau     1620

gcaaaguccc agcccaaaug gccgucgaca ugcaaacucu aaccccagua ggccgccuca     1680

uuacagccaa cccaguaauc acugaaucca ccgagaacag caaaaugaug cucgaacucg     1740

acccaccauu cggggacucc uacaucguca ucggcguagg cgagaagaaa auaacacacc     1800

acuggcaccg cucggggagc acucucggca aggccuucuc aaccacacuc aaaggcgccc     1860

aacgacucgc cgcccuagga gacaccgcau gggacuucgg cucaaucggc ggagucuuca     1920

acagcauagg ccgcgccgua caccaaguau ucggcggagc auuccgcaca cuguucgggg     1980

gaauguccug gauaacacaa ggccucaugg gcgcccuauu acuuuggaug gggguaaacg     2040

cccgagaccg aagcauagcc cuagccuucc uagccaccgg agggguacuc guauuuuuag     2100

ccaccaacgu ccacgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7180
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 2 opt

<400>  7180
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucauaaacgc ccgcaaggaa aagaaaagac gcggggccga cacauccguc ggaauaguag      120

gccugcuacu aacaaccgcc auggccgccg agguaacaag acgcggcagc gccuauuaca      180

uguaccucga ccgcaaugac gcaggagaag caauaucauu ccccaccacc uugggaauga      240

acaaauguua cauccaaauc auggaccuag gacacaugug cgacgcaaca augaguuacg      300

agugcccaau gcuggacgaa ggaguggaac cagacgacgu cgacuguugg ugcaacacca      360

ccagcaccug gguaguauac ggcacaugcc accacaagaa aggcgaagca cgacgaagcc      420

ggagagcggu aacccucccc agccacucca cacgcaaacu gcaaaccaga ucccaaaccu      480

ggcuagaaag ccgcgaauac acaaaacauc ucauacgcgu ggaaaacugg auauuuagaa      540

acccaggcuu cgcccuagcc gccgccgcaa ucgcauggcu ccucggaagc uccaccagcc      600

aaaaaguuau uuaccuggua augauacugu ugauagcacc cgccuacagc auucggugua      660

ucggcguaag caacagagac uucgucgaag gaaugucggg aggcaccugg gucgacaucg      720

uccuagagca cggcggcugc guaaccguca uggcacaaga caaaccaaca gucgacaucg      780

agcucgucac caccaccgua ucaaacaugg ccgaagugcg cagcuacugc uacgaagcaa      840

gcauaucaga cauggcuucu gacagcagau gccccacaca aggagaggcc uaccuagaca      900

agcaauccga cacacaguac guaugcaaac gaacccuagu cgaccgagga uggggaaacg      960

gaugcggauu auucggcaag ggaucccuag ucacgugcgc aaaauucgcc ugcagcaaga     1020

aaaugaccgg aaaauccaua caaccagaaa accucgaaua caggauaaug cucagcguac     1080

acggcucaca acacaguggc augaucguca acgacaccgg acaugaaacc gacgaaaacc     1140

gcgccaaagu ggaaaucacc ccaaacagcc cacgggcaga ggcaacacuc ggcggcuucg     1200

gcucccuagg acuggacugc gaacccagaa ccggacucga cuucuccgac cucuacuacc     1260

uaacaaugaa caacaaacau uggcuggugc acaaagaaug guuccacgac auaccgcuuc     1320

ccuggcacgc cggcgccgac acgggaacac cccacuggaa caacaaagaa gcccucguag     1380

aguucaaaga cgcacacgca aaacgacaaa cggucgucgu ccuaggguca caagaaggcg     1440

ccguccacac cgcacuagcc ggcgcacucg aagcagaaau ggacggcgcc aaaggacgac     1500

ucucaucagg ccaccuaaaa ugccgccuaa aaauggacaa acuacgccug aaaggcguca     1560

gcuacagccu auguaccgcc gcauuuacgu uuaccaaaau acccgcagaa acacuccacg     1620

gcacagucac agucgaaguc caauacgccg ggaccgacgg ccccugcaaa guaccggccc     1680

agauggcagu ggacaugcaa acgcucaccc caguuggaag acugauaacc gcaaaccccg     1740

ucaucaccga gagcaccgaa aacucaaaaa ugaugcuaga acucgaccca cccuucggcg     1800

acuccuacau cgucaucggc gucggugaaa agaaaauaac acaucauugg cacagauccg     1860

gaagcaccau cggaaaagca uucgaagcca ccguccgagg agcaaaaaga auggcggucc     1920

ucggcgacac cgcgugggac uucggaagcg ugggcggcgc ccucaacagc cuagggaaag     1980

ggauacacca aauuuucggu gcagccuuca aaucacuauu cggcggaaug agcugguucu     2040

cccaaauacu aaucgguaca cuacucaugu ggcuaggccu aaacaccaaa aacggcagca     2100

uaagccuaau gugucucgcu cuaggcggcg uacucaucuu ccucucuaca gcagucagcg     2160

ccgacguagg cugcagcguc gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7181
<211>  2521
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7181
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaaugcucg aaaagagaag aaacgacgug gcgcagacac aucggucggc auaguaggac      120

ucuuacucac aaccgcaaug gcagcggaag ucacccgacg cgggagcgcc uacuacaugu      180

accucgaccg caacgaugcc ggugaggcaa ucucauuccc cacgacacua ggaaugaaca      240

aaugcuacau ccaaaucaug gaccucgggc acaugugcga ugcgacuaug uccuaugagu      300

gcccaaugcu ggaugaagga gucgaacccg acgacguaga cugcuggugc aacacgacca      360

guacaugggu cguauacgga acgugccacc acaagaaagg cgaagcccgc cgcagccguc      420

gagcagugac ccuacccagc cacucaacac gcaaacucca aacacguuca caaaccuggc      480

ucgaaagccg agaauacacg aaacaccuaa uaagagucga aaacuggauc uuuaggaacc      540

cgggcuucgc acuagcagcg gccgcgauag ccuggcuccu ggggagcucg accucacaaa      600

aagucauaua ccugguaaug auccuacuga ucgccccggc auacucuaua cgcuguauag      660

gcgucucgaa ccgcgacuuc guagaaggaa ugagcggagg gacauggguc gacauagucc      720

uagaacacgg gggcugcgua acagucaugg cccaagacaa accaaccguc gacaucgaau      780

uagucacaac uacagucucg aacauggccg aaguacggag cuacugcuac gaagccagca      840

ucagcgacau ggccucagac agccgaugcc caacacaagg cgaagcauac cucgacaaac      900

aaagcgacac acaguacgua ugcaaacgca cccuaguaga cagaggaugg ggaaacgguu      960

gcggacucuu cgggaaagga agccuaguca caugcgcaaa auucgccugc agcaagaaaa     1020

ugacuggcaa aaguauacaa ccagaaaacc ucgaauaccg aaucaugcuc agcgugcacg     1080

gaucacaaca cucugggaug auaguaaacg acaccggaca ugaaaccgau gaaaaccgcg     1140

ccaaaguaga gaucacacca aacagcccca gggccgaagc cacccucgga ggcuucggca     1200

gccugggacu agacugcgaa ccccgcacag gccucgacuu cucagaucua uacuaccuca     1260

caaugaacaa caaacacugg cuaguccaca aagaaugguu ccacgacauc ccucuaccgu     1320

ggcacgccgg cgcagacacc ggcaccccac acuggaacaa caaagaagcc cuaguggagu     1380

ucaaagacgc acacgcaaag cgccagacag uaguaguacu agggagccaa gaaggagcag     1440

uccacacagc acucgccggg gcccuagaag ccgaaaugga cggcgcaaag ggacgccugu     1500

ccucgggaca ccuaaaaugc cgacugaaaa uggacaagcu ccgacuaaaa ggcguaagcu     1560

acagucuaug cacagccgcg uucacauuca ccaaaauccc ugcagagacg cugcacggga     1620

ccguaacagu agaaguacaa uacgccggca cggacgggcc cugcaaagua ccagcacaaa     1680

uggccgucga caugcagaca cucacacccg uaggcagacu aaucacagca aaccccguaa     1740

ucaccgaguc gacagaaaac aguaagauga ugcuggaacu cgacccaccc uucggagacu     1800

ccuacauagu aauaggcgug ggugaaaaga aaaucacaca ccacuggcac cgaagcggaa     1860

gcacaauagg caaagccuuc gaagcaacag uaagaggggc aaaacgcaug gccguccucg     1920

gcgacacagc gugggacuuc ggaagcgucg gaggcgcacu caacucccuc ggaaaaggaa     1980

uccaccaaau auucggagca gccuucaaau cccuauucgg cgggaugagu ugguucagcc     2040

aaauacugau aggcacccug cuaauguggc uaggacucaa caccaaaaac gggucaauau     2100

cacucaugug ccucgcgcua ggaggagucu uaaucuuccu aucaacagcu gucagcgccg     2160

auguaggaug cuccguugac uuuucaaaau gaggacuagu gcaucacauu uaaaagcauc     2220

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2280

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2340

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2460

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2520

u                                                                     2521


<210>  7182
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7182
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcaaggaa ggagaagaaa cgccgagggg ccgacacgag cgugggcaua gucggccucc      120

uacucacaac cgcgauggcg gcagagguaa caagaagagg cagcgccuac uacauguacc      180

ucgaccgcaa cgacgcagga gaagcaauau cauucccaac cacccuaggc augaacaaau      240

guuacauaca aaucauggac cuaggccaca ugugcgacgc cacaaugucc uacgaaugcc      300

caaugcuaga cgagggcgua gagcccgacg acgucgacug cuggugcaac accacuagca      360

ccuggguggu auauggcacu ugccaucaca agaaaggaga agcccgccgc uccaggagag      420

caguaacccu ccccagccac agcacaagaa aacuccaaac aagaucccaa acuuggcugg      480

aaucgagaga auacaccaaa caucuaauaa gagucgagaa cuggaucuuc cgaaacccag      540

gauucgcacu agccgcagca gcaauagccu ggcuucuagg cucaagcaca ucccagaaag      600

uaaucuaccu agucaugaua cucuugauag caccagcaua cucaauccgc ugcauaggag      660

uaagcaaccg cgacuucguc gaaggaauga gcggaggcac augggucgau aucguacucg      720

agcacggagg cugcguuacg gucauggcac aagacaaacc caccguagac aucgaacugg      780

uaaccacaac agugagcaac auggcagaag uacgcagcua cuguuacgaa gcaucaaucu      840

cagacauggc aucagacagc cgaugcccaa cacaaggcga agcguaccuc gacaaacaaa      900

gcgacacaca auacguaugu aaacggaccc uggucgaccg aggauggggc aacggaugcg      960

gacuguucgg caaaggaucg cucguaacau gcgcaaaauu cgcaugcagc aagaaaauga     1020

ccggaaaaag cauacaaccc gagaaccucg aauaccggau caugcucucg guacacgggu     1080

cccaacacag cggcaugaua guaaacgaca cgggacacga aacagacgag aacagagcga     1140

aagucgaaau aacaccaaac agcccccgag cagaagcgac ccugggcggc uucggguccc     1200

ugggacuaga cugcgaacca agaacuggac uagacuuuuc cgaccuauac uaccuaacca     1260

ugaacaacaa acacuggcug guacacaaag aaugguucca ugacaucccg cucccauggc     1320

acgccggggc cgauaccggc acaccccacu ggaacaacaa agaagcccua gucgaauuua     1380

aagacgccca ugccaaaaga caaacagucg ucguacuggg cagccaagag ggcgccguac     1440

acaccgcgcu cgcaggcgca cuagaagccg aaauggacgg agcaaaagga agacuaagcu     1500

caggccaccu aaaaugcaga cucaaaaugg acaaacugcg ccugaaagga gugucguacu     1560

cccuaugcac agcagccuuc accuucacaa aaaucccagc agagacgcua cacgggacag     1620

ucaccguaga ggugcaauau gcagggacag acggcccaug caaaguaccc gcccaaaugg     1680

ccguagacau gcaaacccua acaccagucg gacgccucau aacagcaaac ccaguaauaa     1740

cagaaucuac ggagaacucg aaaaugaugc uggaacuaga uccgcccuuc ggcgacagcu     1800

acaucguaau aggagucggu gaaaagaaaa uaacacacca cuggcacaga uccggcagca     1860

cgauaggaaa agccuucgag gcaacaguaa ggggcgccaa acgaauggca guacuaggcg     1920

acaccgccug ggacuucggg ucagucggag gggcacuaaa cagucucggg aaaggcauac     1980

accaaauuuu cggagccgcc uucaaaagcc uauucggagg aaugucgugg uucucacaaa     2040

uccuuauagg cacgcuacua auguggcuug ggcuaaauac caaaaacggc agcaucagcc     2100

ugaugugccu agcacucgga ggcgugcuca uauuccucag caccgccguc agcgcggacg     2160

ucggaugcag cguggacuuc agcaagugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  7183
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7183
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cgcgaaaaga aaagaaaagg cgaggggccg acacaagcgu uggaaucguc ggccuacucu      120

ugacaacggc aauggcagca gaaguaacac ggcgcggauc agcguacuac auguaccucg      180

acagaaacga cgccggcgaa gccaucagcu uccccacgac ccucggcaug aacaaaugcu      240

auauacaaau cauggaccuc ggccacaugu gcgacgcaac aaugagcuac gaauguccaa      300

ugcucgacga aggggucgaa ccagacgacg uagacuguug gugcaacaca acgagcacau      360

ggguaguaua cggaacaugc caccacaaga aaggcgaagc cagacgcagc cgaagggcag      420

uaacacuccc cagccacuca acacgcaaac uccaaacccg aucacaaacg uggcuagaau      480

cccgugaaua caccaaacac cuuauacgcg ucgaaaacug gaucuuccgc aauccagggu      540

ucgcacucgc cgcagcagcc aucgcguggc ugcuaggauc cagcacaucg caaaaaguaa      600

ucuaccuagu aaugauccuc cuaauugccc cggcguacuc aauccgaugc auaggagucu      660

caaaccggga cuucguagaa ggcaugagcg gaggcacaug gguagacauu guccuagaac      720

acggcgggug ugucaccguc auggcccaag acaagccaac cguggacauc gaacucguca      780

caacaaccgu aucuaacaug gcagagguaa gaucauacug cuacgaagcc aguauauccg      840

acauggcaag cgauuccaga ugcccaaccc aaggcgaagc cuaccuagau aaacaaucag      900

acacccaaua cguaugcaaa cgcaccuuag ucgaccgagg cuggggcaau ggaugcggcc      960

ucuucgggaa aggaagccua gucacuugug cgaaauucgc cugcagcaag aagaugacag     1020

gcaaauccau ccagcccgaa aaccucgaau accgaaucau gcuaagcgua cacggcagcc     1080

aacacucugg aaugauaguc aacgacacug ggcacgaaac agacgaaaac cgcgcgaaag     1140

uggaaaucac cccaaacuca cccagagccg aagccacacu aggaggcuuc gguucacucg     1200

gacucgacug cgaaccaaga accggccuug acuucucaga ccuauauuau cuaacaauga     1260

acaacaagca cuggcuggug cacaaagaau gguuccacga cauaccucuc ccguggcacg     1320

caggcgcaga cacaggaaca ccacacugga acaacaaaga agcccuaguc gaguucaaag     1380

acgcacacgc caaacgacag acuguagucg ugcuaggaag ccaagaagga gccgugcaca     1440

cugcccucgc aggggcacua gaagcagaaa uggacggagc caaaggaaga cuaagcagcg     1500

gacaccugaa augcagacua aaaauggaca aacuaaggcu caaaggaguc aguuacucac     1560

uaugcacagc cgcguucaca uucacaaaaa uacccgcaga aacgcuccac ggaacaguca     1620

caguugaagu ccaauacgca ggcacagacg gccccugcaa agugcccgcc caaauggcag     1680

uagacaugca aacacucacg cccguaggga gacuaauaac ggcaaacccg gucauaaccg     1740

aaagcaccga aaacagcaaa augaugcucg aacucgaucc gccguucggc gauagcuaca     1800

ucgucauagg agucggcgaa aagaaaauca cacaccacug gcacagaucc ggauccacaa     1860

ucggaaaagc cuucgaagcc acagugcgag gagccaaacg uauggcggua cucggcgaca     1920

cggccuggga cuucggcagc gucgggggag cacugaacag ccuaggaaag ggaauccacc     1980

aaaucuucgg agccgccuuu aaaagucucu ucgggggcau gucaugguuc agucaaauac     2040

ucaucggaac acuacucaug uggcuaggcc uaaacaccaa aaacggaucc auaucacuaa     2100

ugugccuugc ccuaggcgga guccuaauau uccucucaac agcagucagc gccgacgucg     2160

ggugcucggu cgacuucuca aaaugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  7184
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7184
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

gaaaagagaa aaagagacga ggagcggaca cguccgucgg caucgucggu cuccuucuga      120

caaccgccau ggccgcggag guaacccgcc gcggaagcgc cuacuauaug uaccucgaca      180

gaaacgacgc cggagaagca aucucauucc ccacaacccu aggaaugaac aaaugcuaca      240

uccaaaucau ggaccucggc cacaugugcg acgccacaau gagcuacgag ugccccaugc      300

uagacgaagg aguagagccc gacgacgucg acugcuggug caacaccacc ucaaccuggg      360

uagucuacgg aacaugccac cacaagaaag gcgaggcaag acgcucccgc cgagccguua      420

cgcuuccguc acacagcacc agaaaacucc aaaccagauc ccagaccugg cucgaaucca      480

gagaauacac uaaacaccuu auacgagucg agaacuggau cuucaggaac ccaggcuucg      540

cguuagcagc agccgcgauc gccuggcuac ucggcaguuc caccagccaa aaaguaauuu      600

accucgugau gauccuacua aucgcaccgg cauauucuau aagaugcaua ggcgucagca      660

acagggacuu cguggaagga augucaggag gcaccugggu cgauaucgua cuagaacacg      720

gaggcugcgu caccgucaug gcccaagaca aaccaacggu cgacaucgaa uuagucacca      780

caaccguaag caacauggca gaagucagaa gcuacugcua cgaagcaucc aucuccgaca      840

uggcaagcga cucacgcugc cccacccaag gcgaagcaua ccucgauaaa caaagcgaca      900

cacaauacgu augcaaaagg acacucgugg acagaggaug ggggaacggc ugcggccuau      960

ucggcaaagg aagccucgug accugcgcca aauucgcaug cuccaagaaa augaccggaa     1020

aaucaaucca accagaaaau cucgaauaca gaaucaugcu cuccguccac gggucccaac     1080

acagcggcau gauaguuaac gacacaggcc augagaccga cgaaaaccgg gcuaaaguag     1140

agauaacccc caacucaccc cgggccgaag ccacgcuagg gggcuucgga agccuaggac     1200

ucgacuguga accgcguacg ggacucgacu ucuccgaccu guauuaucuc acaaugaaca     1260

acaaacacug gcuaguacac aaagaguggu uccacgacau accgcuacca uggcacgccg     1320

gcgccgacac cggaacacca cacuggaaua acaaagaagc ccucguagag uucaaagacg     1380

cgcacgcaaa aagacaaacc gugguagucu uaggcucaca ggaaggggca guacacacag     1440

cccuggcagg agcacuagaa gccgaaaugg auggagcaaa agggcgacua agcagcgggc     1500

accuuaaaug ccgacucaaa auggacaaac uacgccucaa aggagucucc uacucgcuau     1560

guaccgccgc auuuacauuc accaaaaucc ccgcagaaac ccuccacgga acaguaacag     1620

uagaagucca auaugccggc acagauggcc caugcaaagu accugcgcaa auggcagucg     1680

acaugcaaac acuaacgccu gucggccgac uaaucacagc caacccagua aucacagaau     1740

cgaccgaaaa cagcaagaug augcuagaac uagacccacc cuucggcgac uccuacauag     1800

ucauaggggu aggagaaaag aaaaucacuc accacuggca caggagcggc uccaccauag     1860

gaaaagcguu cgaagcuacc gucagaggcg caaaacgcau ggcgguacug ggagacaccg     1920

caugggacuu cggaagcguc ggaggggccc uaaacucccu aggaaaagga auccaccaaa     1980

uauucggagc cgccuucaaa agccuauucg gaggcaugag cugguuuucc caaauacuaa     2040

ucggcacccu ccucaugugg uuaggacuaa acaccaaaaa cggcagcaua ucacucaugu     2100

gccucgcgcu aggcggcguc cucauauucc ucagcacagc agugucggcc gacgucggcu     2160

gcagcguaga cuuuucaaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  7185
<211>  2509
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7185
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aagagaagaa acgaagagga gcagacacca gcguaggaau agucggacuc cuccuaacua      120

ccgcaauggc agcagaaguc acgcgaagag gcagcgcgua cuacauguac cuagaccgga      180

acgaugccgg cgaggccauc ucauucccaa caacacucgg gaugaacaaa ugcuacauac      240

agaucaugga ccucgggcac augugcgacg ccacuauguc cuacgagugc ccgaugcugg      300

acgaaggcgu cgagccagac gacgucgacu guugguguaa cacuaccagc accuggguag      360

uauacggaac augccaccac aagaaaggag aagcccgcag gagcagacgc gcggucacgc      420

ugccauccca cagcacgcgc aaacuccaaa cuaggucaca aaccuggcua gagagccgag      480

aauacaccaa gcaccuaauc agagucgaaa acuggauauu ccgcaaccca ggauuugcac      540

uagcggccgc cgcaauagcg uggcuacuag gcucgagcac aucccaaaaa gugaucuacc      600

uagucaugau acuccuaauc gcacccgcau acagcauccg augcauagga gucucuaaca      660

gagauuucgu cgaaggaaug ucgggcggaa cgugggucga cauaguacua gaacacggag      720

gcugcguaac cgugauggcc caagacaaac caaccgucga caucgagcua gucacgacua      780

ccguaagcaa cauggcagaa guacgauccu acugcuacga agcaagcauc ucagacaugg      840

ccagcgacag ccgaugcccc acccagggcg aagcauaccu agacaaacaa ucagacacac      900

aauacgucug caaaagaaca cucguagauc gcggaugggg caacggaugc ggacucuucg      960

gaaaaggcag ucuagucaca ugcgccaaau ucgcaugcuc aaagaaaaug accgguaaau     1020

ccauacaacc agaaaaccuc gaauaccgga uaaugcucuc aguacauggg agccaacaca     1080

gcggcaugau agucaacgac acagggcacg aaacugacga aaaccgcgcc aaaguagaaa     1140

uaacaccaaa cagcccccgc gccgaagcca cacugggagg cuuuggaagc cucggcuuag     1200

auugcgagcc acgaacaggc cuagacuucu cggaccuaua cuaccucacg augaacaaca     1260

aacacuggcu aguacauaaa gaaugguucc augacauccc ccucccaugg cacgcuggcg     1320

cggacacggg gacaccacac uggaacaaca aggaagcccu gguagaguuc aaggacgccc     1380

acgcgaaaag gcaaacaguc gugguauuag gcagccagga aggcgccguc cacaccgcau     1440

uagccggcgc ccuggaagcc gaaauggacg gagcaaaagg cagauuaagc uccggacacc     1500

ucaaaugccg gcuaaagaug gacaaacuca gauugaaagg aguauccuac agccuuugca     1560

ccgcagccuu caccuucacg aaaauaccag ccgaaacacu acacggaaca guaacggucg     1620

aagugcaaua cgcaggaaca gacggccccu gcaaaguacc agcgcaaaug gcagucgaca     1680

ugcaaacccu gacgccgguc ggcagacuaa ucaccgcaaa cccagucauc acagaaucca     1740

ccgaaaacuc aaaaaugaug cuagaacugg acccacccuu cggagacucc uacauuguaa     1800

uaggagucgg cgaaaagaaa auaacccacc acuggcaccg aucaggcagc acaauaggca     1860

aagcauucga agccaccgua agaggcgcaa aacgcauggc aguacuggga gacaccgcau     1920

gggacuucgg cagcguuggc ggagcccuca acuccuuagg caaaggaauc caccaaauau     1980

ucggagcagc auucaaaagc cuguuuggcg guaugagcug guucucccaa auacucaucg     2040

ggacacugcu gauguggcuc ggacucaaca cgaaaaacgg uagcauaagu cugaugugcc     2100

ucgcccuagg cggcguacuc aucuuccuaa guacagcggu aucagcagac guaggcugca     2160

gcgucgacuu cagcaaauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2220

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2280

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2340

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2460

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2509


<210>  7186
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7186
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaag       60

aaaagaaacg ccgaggcgca gacaccagug uaggcauagu aggacuucuc cuaacaacag      120

ccauggcggc ggaaguaacu cgcagaggcu ccgccuacua cauguaucuc gaccgaaacg      180

acgcggguga agcaaucagc uucccaacca cucucggcau gaacaaaugc uacauccaaa      240

ucauggaccu cggccacaug ugcgacgcca caaugagcua ugaaugcccc augcuagacg      300

aaggcguaga acccgacgac guagacugcu gguguaacac caccucaacc ugggucgucu      360

acggaacgug ccaccauaag aaaggagaag cacgccgcag cagacgcgcg gucacauuac      420

caagccauag cacgcgcaaa cuacaaaccc ggucacagac auggcucgag agccgagagu      480

acaccaaaca ccuaauacgc gucgaaaacu ggauauuccg aaaccccgga uucgcacuug      540

cagcggccgc caucgcaugg cuccugggcu caagcaccag ccaaaaagua aucuaccuag      600

ucaugauccu gcuaaucgcc ccagccuaca gcaucaggug cauaggcgug agcaaccgag      660

acuucgucga aggaaugucg ggcggaaccu ggguagacau cguacuggaa cauggcggcu      720

gcgucaccgu aauggcccaa gacaagccca ccgucgacau agaacucguc acaacuaccg      780

ucuccaacau ggccgaggua cgcuccuacu gcuacgaagc cucaaucucc gacauggcca      840

gcgacucacg augcccuacg caaggcgaag ccuaccugga caaacaaucg gacacacaau      900

acgugugcaa acgaacauua gucgaccgag gaugggggaa cggcugcggc cuguucggca      960

aaggcucauu agugaccugc gcaaaguuug ccugcagcaa aaagaugacc ggaaaaucca     1020

uacaaccaga aaaccuagaa uacagaauca ugcugucagu acauggcuca caacacagug     1080

guaugaucgu caacgacacg ggccacgaaa cagacgaaaa ccgagcgaaa guagaaauca     1140

ccccaaacag cccgcgcgcu gaagccacac ucggagguuu cggcucacua ggccuagacu     1200

gcgagccacg aacuggccuu gacuucuccg accuguacua ccucaccaug aauaacaaac     1260

acuggcuagu acacaaagaa ugguuccacg acaucccacu acccuggcac gccggcgccg     1320

acaccgggac cccacacugg aacaacaaag aagcacuggu agaguucaaa gacgcccacg     1380

ccaaaagaca aaccgucgua guacuaggca gccaagaagg ugcgguccac accgcccuag     1440

ccggcgcccu agaagccgaa auggacggug ccaaaggccg ccuuagcucc ggucaccuaa     1500

aaugccgacu aaaaauggac aaacugagac uaaaaggcgu cucauacuca uuaugcacag     1560

cagccuuuac uuucacaaag aucccagccg agacgcuaca cggaacagua acaguagaag     1620

uacaguacgc cgggaccgac ggccccugca aaguccccgc ccaaauggcc guagacaugc     1680

agacacuaac cccagucggg agacugauca ccgccaaccc gguaauaaca gaaagcaccg     1740

aaaacuccaa aaugaugcuu gagcuagacc caccuuucgg ggauagcuac aucgucaucg     1800

ggguaggcga gaagaaaaua acacaucacu ggcauagaag cggcagcacg auaggaaaag     1860

cauucgaagc aacaguccgc ggcgcaaaac gcauggccgu uuugggcgac acagcauggg     1920

acuucggcuc cgugggcggc gcacucaacu ccuuaggcaa aggcauccac cagaucuucg     1980

gggccgccuu caaaucacua uucggcggca ugucaugguu cagccagauc cucauaggca     2040

cacuccuaau guggcuggga cuaaauacca aaaacggcuc caucucacuc augugccuug     2100

cccucggagg cguccuaauc uuccuuagua cagccguaag cgccgacguc ggaugcuccg     2160

uagacuucuc caaaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7187
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7187
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaaa       60

agaaaagacg cggcgccgac acgagcgucg guauaguagg acuucuacuc acgaccgcca      120

uggcggccga aguaaccaga cggggcagcg ccuacuacau guaucucgac cgcaacgacg      180

ccggcgaagc caucucguuc cccaccacac uagggaugaa caaauguuac auccaaauaa      240

uggaccucgg gcacaugugc gacgcgacaa ugaguuacga augcccaaug uuagacgaag      300

gcguagaacc ggacgacguc gauugcuggu guaacaccac aucaaccugg guagucuacg      360

ggaccugcca ccacaaaaag ggcgaagccc gaagaucacg gagagccguu acccuaccga      420

gccacuccac aagaaaauua caaacacgaa gccagaccug guuagaauca agagaguaca      480

caaaacaccu gauaagagua gagaacugga ucuuccgaaa cccgggcuuc gcacuagccg      540

ccgccgcaau cgccuggcuc cuaggauccu caaccagcca aaaagucauu uaccucguaa      600

ugauccuccu cauagcacca gcauacucca uccgaugcau cgguguuucc aacagagacu      660

ucguagaagg caugucgggc ggcaccuggg ucgacaucgu ccuagagcac ggaggcugcg      720

ugacugucau ggcacaagac aagcccaccg ucgacaucga gcuaguaacc acaacaguau      780

ccaacauggc ugaaguccga agcuacugcu acgaagcauc aaucucugac auggccagcg      840

acagccgaug ccccacacaa ggagaagccu accuagacaa acaaagcgac acccaauacg      900

ucugcaagcg cacccuaguc gaccgcggau ggggaaacgg gugcggacuc uucgggaagg      960

gaagccuagu aacaugcgcc aaauucgcgu gcucaaagaa aaugacgggc aagaguaucc     1020

agcccgaaaa cuuagaauac agaaucaugc ucucgguuca cggaucccaa cacagcggca     1080

ugauaguaaa cgacaccggc cacgaaacag acgaaaacag ggcaaaagua gaaaucaccc     1140

ccaacucacc cagagccgaa gccacgcucg gcggcuucgg gucacucggg cucgacugcg     1200

agccccgaac cggacuugac uucagcgacc uuuacuaccu aacaaugaac aacaagcacu     1260

gguuagugca caaagagugg uuccacgaua ucccacuccc cuggcacgca ggcgcagaca     1320

ccgguacacc ccacuggaac aacaaagaag cacucguaga guucaaagau gcccacgcaa     1380

aaaggcaaac cgucguagua uuaggcagcc aagaaggcgc aguacacacc gcccucgccg     1440

gagcccuaga agcagaaaug gacggcgcaa aaggaagacu cuccucaggc caccucaaau     1500

gccgauugaa gauggacaaa cuucgacuca agggagucag uuacucauua ugcacugcag     1560

ccuucaccuu caccaaaauc ccggcagaaa cgcuacacgg aaccguaacc gucgaagucc     1620

aauaugcagg caccgacggc ccgugcaaag uacccgccca gauggcgguc gacaugcaaa     1680

cgcugacacc gguaggucgc cucauaaccg ccaaccccgu uauaacggaa uccaccgaaa     1740

acuccaaaau gaugcucgaa cuagacccgc ccuucggaga cagcuacauc gugaucggug     1800

ucggagaaaa gaaaauaacc caccacuggc acagaagugg gagcacaauc ggaaaagccu     1860

ucgaagccac cgucagaggc gccaaacgca uggccguccu cggcgacaca gcaugggacu     1920

ucgggagcgu cggcggugcc cuaaacagcc ucggcaaagg cauacaccaa auauucggag     1980

cagcuuucaa gagcuuauuc gggggcauga gcugguucuc ccaaauccua aucgggaccc     2040

uacucaugug gcucggacua aacaccaaaa acggaagcau uagccucaug ugccuggccc     2100

ucgggggcgu acucaucuuc cugucgaccg ccguaagcgc ugaugucgga ugcuccguag     2160

acuucucaaa augaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  7188
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7188
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aacgacgggg cgcagacaca uccgucggca ucguaggacu ccugcuaacc acagcaaugg      120

cagccgaagu cacacgcagg ggcucugcau acuauaugua ccuagaccgg aacgacgcag      180

gagaagccau aucguuccca accacacugg gaaugaacaa augcuauaua cagauuaugg      240

accucggcca caugugcgac gccaccauga gcuacgaaug cccuaugcuc gacgagggcg      300

uggaaccaga cgacgucgac ugcuggugca acacgacaag caccugggug gucuacggaa      360

ccugccacca caagaaaggc gaggcgagac gcucccgacg cgcggucacc cucccaagcc      420

acucaacgcg caaacuacag acaagauccc aaacauggcu cgaaagccgc gaauacacca      480

aacaccuaau ccgagucgaa aacuggaucu uccgcaaccc cggauucgca cuagccgccg      540

ccgcaauagc auggcucuua ggcucaagca ccagccaaaa aguaaucuac cucguaauga      600

uccuucucau cgcaccagcc uauucaauaa gaugcauagg aguuagcaac agagacuucg      660

uagaaggaau gucgggagga accugggucg acaucguccu cgagcacggg ggcugcguca      720

ccgucauggc ucaggacaaa ccaacagucg auaucgagcu agucaccacg acaguaagca      780

acauggcaga aguacggucc uacugcuacg aagcaucgau cuccgacaug gcaucagacu      840

cacgaugccc aacccaaggc gaagcauacc uagacaagca aucagacacg caguacguau      900

gcaagcgaac acugguagac agaggcuggg gcaacggaug cggccuauuc ggaaagggcu      960

cacugguaac cugugcaaaa uucgcaugcu caaagaaaau gacaggaaaa agcauacaac     1020

ccgaaaaccu cgaguaccgu auaaugcucu caguccacgg aagccaacau uccggcauga     1080

ucguaaacga cacaggacac gagaccgacg aaaacagagc caaagucgaa aucacgccca     1140

auagcccacg cgccgaagcc acccuaggag gcuucggauc acuaggauug gacugcgaac     1200

cacguaccgg ccuagacuuc ucagaccuau acuaccuaac caugaauaac aaacacuggu     1260

uagugcacaa agagugguuc cacgacauac cacucccaug gcacgcgggc gcggacacag     1320

gcacacccca cuggaacaac aaagaagccc uaguagaguu caaagacgca cacgcaaaac     1380

ggcaaaccgu aguaguccug ggcagccaag aaggcgccgu acacaccgcg cuggccggag     1440

cccucgaagc agaaauggac ggcgcgaaag gaagacuauc cuccggccac cuaaaaugcc     1500

gacucaaaau ggacaagcua aggcugaaag gcgucagcua cucccucugu accgcagcgu     1560

uuaccuucac caaaauccca gcagaaaccc uccacggaac agugacagua gaaguccaau     1620

acgccggcac agauggcccu ugcaagguac cagcgcaaau ggcggucgac augcaaacac     1680

uaacaccggu cggccgccuc auaacagcca aucccguaau cacagaaagc accgaaaaca     1740

gcaaaaugau gcucgaacua gacccgccau ucggcgacag cuacaucgug auaggagucg     1800

gcgaaaagaa aauaacacau cauuggcaca gaagcggcuc cacaaucggc aaagcauucg     1860

aagcaacagu aagaggcgcc aaacgaaugg caguccuggg ugacacagcg ugggauuucg     1920

ggucagucgg aggugcccuu aacagccuag gaaaagggau ccaccaaaua uucggggccg     1980

cauucaaauc acuuuucggg ggaauguccu gguucuccca aauccucauc ggaacacucc     2040

uaauguggcu cggacucaac accaaaaacg gcagcaucag ccucaugugc cucgcacucg     2100

gaggcguacu aauauuccug ucaacggcag uuagcgcaga cguagggugc ucagucgacu     2160

ucagcaaaug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  7189
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7189
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

ggagaggggc ggacaccagc gucggcauag ucggccuacu acucacgacc gcaauggccg      120

ccgaaguaac acguagagga ucggccuacu acauguaccu cgacaggaac gacgccggag      180

aagccaucuc auuccccaca acacugggaa ugaacaaaug uuacauccaa aucauggacu      240

uaggacacau gugcgacgcc accaugucau acgaaugccc caugcucgac gaaggcgugg      300

aacccgacga cgucgacugc ugguguaaca caacaucaac cuggguaguc uacggcacau      360

gccaccacaa gaaaggcgaa gcccgucgca gcagaagggc aguaacccuc ccaagccacu      420

caacgcggaa acugcaaacc cgaagucaaa ccuggcugga aucgagggaa uacacaaaac      480

accuaauccg aguagaaaac uggaucuucc gcaauccagg cuucgcacuc gccgcagcag      540

ccauagccug gcuacucggc agcuccacaa gccaaaaagu aauuuaccuc gucaugauac      600

uacugaucgc ccccgccuac uccaucagau gcaucggcgu cucaaaccgc gauuucgucg      660

aaggcauguc agguggcacc uggguagaca ucguccucga acacggaggc ugcgucaccg      720

ucauggcaca agacaaaccc acagucgaca ucgaauuggu cacaaccacc gucagcaaca      780

uggccgaagu acgaagcuac ugcuacgaag cgucaauauc cgacauggcg agcgacuccc      840

gauguccaac ucaaggagaa gcguaucucg acaagcaauc ggacacccaa uacguaugua      900

aacgaacccu aguagaccgc ggauggggca acggaugcgg ccuauucgga aaaggcuccc      960

ucgugacaug ugcaaaguuu gccugcagca agaaaaugac aggcaaaagc auccaaccag     1020

aaaaccuaga auaccgaauc auguugucag uacacggcuc ccaacacagu ggaaugauag     1080

ugaacgacac aggccacgaa acagacgaga aucgcgccaa agucgagaua accccuaacu     1140

caccacgcgc agaagcgaca cugggaggau ucgggucccu aggccucgac ugcgaaccac     1200

gcaccggcuu agacuucagc gaucucuacu accuaaccau gaacaacaaa cacuggcucg     1260

uacacaaaga augguuccac gauaucccac uccccuggca cgccggcgcu gacacaggca     1320

cgccccacug gaacaacaaa gaagcgcuug uggaguucaa ggacgcccac gcaaaaaggc     1380

aaaccgucgu aguacuaggc ucacaagagg gcgccguaca caccgcccug gcaggcgcac     1440

ucgaagccga aauggacggc gccaaaggac ggcucuccag cggacaccuc aagugccgcc     1500

uaaaaaugga caaacugaga cuaaaaggcg uaucauacag ccugugcacc gccgcauuca     1560

ccuucaccaa aauacccgcg gaaacccuac acggcacagu aaccguagaa guacaauacg     1620

caggcaccga cggccccugc aaaguaccgg cccagauggc cguagacaug caaacccuca     1680

cucccgucgg acgccucauc acagcaaacc ccgucauaac agaauccacc gaaaacagca     1740

aaaugaugcu cgaacucgac ccacccuucg gagacuccua cauaguaauc ggagucggcg     1800

agaagaaaau cacccaccac uggcaucgau caggaucaac gaucggcaaa gcauucgaag     1860

caaccgugcg gggagcaaaa aggauggccg uacucggcga caccgccugg gacuucgggu     1920

cagucggggg cgcccuaaac ucccugggaa aaggcauuca ccaaaucuuc ggugccgccu     1980

ucaaaagccu guucgggggc augagcuggu ucagccaaau acucauaggc acacuccuga     2040

uguggcuagg ccucaacacc aagaacggca gcaucaguuu aaugugccua gcccugggag     2100

gaguccucau cuuccucagc acagccguau cagcagacgu aggcuguagc gucgacuuca     2160

gcaaaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  7190
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7190
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

ggggcgcaga cacaucagug ggcaucguag gccuccugcu caccaccgcg auggccgcag      120

aggucacccg acgcgggagc gccuacuaca uguaccuaga ccgaaacgau gccggcgaag      180

ccaucucguu cccgacaacc cugggcauga auaaaugcua cauccaaaua auggaccuag      240

gucacaugug cgacgcaacu augucuuacg aaugcccgau gcucgacgag gguguggaac      300

cugaugacgu agacugcugg ugcaacacca caagcacaug ggugguauac ggaacaugcc      360

accacaagaa aggcgaagca cgacggagcc ggcgcgcagu cacccuaccg agccacucaa      420

cccgaaaacu ccaaacccgc agccaaacau gguuggaaag ccgcgaauac acaaaacacc      480

uaauccgcgu cgaaaacugg aucuuccgca acccgggcuu cgcccucgca gccgcagcca      540

ucgccuggcu uuuaggcuca uccaccucac aaaaaguaau cuaccuaguc augauccuac      600

ucauagcccc ugcauacucc aucaggugca uaggcguauc caacagggac uucgucgaag      660

gcaugagcgg cggcaccugg gucgacaucg uacucgaaca cgggggcugc guaacaguca      720

uggcacaaga caaacccaca gucgacaucg aacucgugac aaccacaguc agcaacaugg      780

cagaaguaag aucauacugc uacgaagcau ccauaagcga cauggcaucu gacagcagau      840

gcccaacaca gggagaagca uaccucgaca aacaaagcga cacacaauac gucuguaaac      900

gaacccuagu agaccgcgga uggggaaacg gcugcggacu cuucggaaaa ggcagccucg      960

uaacaugcgc caaauucgcc ugcagcaaga aaaugaccgg caaauccaua caacccgaaa     1020

accucgagua cagaaucaug cuuucagucc acggaagcca acauuccggc augauaguca     1080

acgacaccgg acacgaaacc gacgaaaaca gagcgaaagu cgaaaucaca cccaacucac     1140

ccagggcgga agcaacacug ggaggauucg ggucacucgg ucuugauugu gaaccccgaa     1200

cuggacucga cuucuccgac cucuacuacc ucaccaugaa caacaaacac uggcuagucc     1260

acaaagagug guuccacgac aucccacuac ccuggcacgc aggagccgac acaggcaccc     1320

cacacuggaa caacaaagaa gcacuggucg aguucaaaga cgcacacgcc aaacgccaaa     1380

cugucgucgu acuaggcagc caggaaggag caguccacac agcacuggcc ggagcucuag     1440

aagcagagau ggacggcgcg aaaggacgcc uaagcucagg ccacuuaaaa ugccgacuca     1500

aaauggacaa acuccgucuu aaaggagucu cguacucacu gugcaccgcu gcauucaccu     1560

uuaccaagau ccccgcggaa acccuacacg gaaccgucac aguagaaguc caauacgccg     1620

gaacugacgg cccaugcaaa guccccgccc aaauggcagu ggauaugcaa acccuaaccc     1680

ccguaggacg ccuaauaaca gccaaccccg uuaucaccga auccaccgaa aacucuaaaa     1740

ugaugcugga acuagaucca cccuucggag acuccuauau aguaaucggc gucggagaaa     1800

agaaaaucac gcaccacugg caccggagcg gcucaaccau cggaaaagcc uuugaggcga     1860

ccguccgcgg agccaaacgc auggcaguac ucggagacac agccugggac uucgggagcg     1920

ucggaggcgc ccuaaacuca cucggcaaag gcauccacca aaucuucgga gccgcauuca     1980

aaucccucuu cggcggcaug agcugguucu cacagauacu aauaggaacc cuccuaaugu     2040

ggcucggccu caacaccaaa aacgguagca ucagccuaau gugccuagca cuaggcggcg     2100

uacuaauauu ccucuccacc gccgucagcg ccgacguggg cuguuccguc gacuucucca     2160

aaugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  7191
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7191
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gcgccgauac uagcguagga aucgugggac uccuacucac aacugcaaug gccgcagaag      120

ucacaagacg aggaagcgca uacuacaugu aucuagaccg gaacgacgcc ggagaagcaa      180

ucagcuuccc gacaacacua ggaaugaaua aaugcuauau ccaaauaaug gaccuaggcc      240

acaugugcga cgcgaccaug agcuacgagu gccccaugcu cgaugaaggc gucgaaccag      300

acgacgucga cugcuggugc aacacuacaa gcacaugggu aguauacggg acaugucacc      360

acaagaaagg agaagccaga cgcucgcgcc gcgcagucac acuccccuca cacucaacac      420

ggaaacuaca aacgcgcucg caaaccuggu uggaaucccg agaauacacc aaacaccuaa      480

uccgcgucga aaacuggauc uuccgcaacc cggguuucgc acucgcagcc gcagccaucg      540

ccuggcuacu cggcagcuca acaagccaaa aagucauaua ccuaguaaug auacuacuga      600

ucgcaccagc cuacagcauc agaugcaucg gcgucucaaa ccgcgacuuc gucgaaggaa      660

uguccggcgg aaccugggua gacaucguac uagaacacgg cggaugcguc accgucaugg      720

cacaagacaa acccacagua gacauugagc ucguaacaac aacagucucc aacauggccg      780

agguccgauc auacugcuac gaagcaucca ucagcgacau ggccagcgac uccaggugcc      840

cgacccaagg cgaagccuau cucgacaaac agagcgacac gcaguacguc ugcaaacgaa      900

cauuagucga ccgaggcugg ggaaacggau gcggccuauu cggaaaagga agccuaguca      960

cgugcgcaaa auucgcaugc ucaaagaaaa ugaccggaaa aucgauccaa cccgaaaacc     1020

uggaauaccg aauaaugcuc uccguacacg ggagccagca cucaggaaug auagucaaug     1080

acaccggcca cgaaacagac gagaaucgag cuaaaguaga gauaacaccc aacagcccca     1140

gagcagaagc gacccucggc ggcuucggcu cccucggcuu agacugcgaa ccacgcaccg     1200

gccucgacuu cagcgaccua uacuauuuaa caaugaacaa caaacacugg cuagugcaca     1260

aagaaugguu ccacgacaua ccacuaccau ggcacgcagg ggcagacacg gggaccccac     1320

acuggaacaa caaagaagcc cuaguagagu ucaaagacgc acacgcaaaa cgacaaacag     1380

uagucguacu ugggagccag gaaggcgcag uccacaccgc acuagccggg gcccucgaag     1440

cagaaaugga cggggcaaaa ggcagacuaa gcagcggaca ucugaaaugc agacucaaaa     1500

uggacaaacu ccgccuaaaa ggcguguccu acucacuaug caccgcagcc uucaccuuca     1560

cgaaaauccc agcggaaacc uuacacggca ccgucacagu ggaaguacaa uacgccggca     1620

ccgacggacc augcaaagua ccagcacaga uggccgucga uaugcaaaca cugacgcccg     1680

ucggacgacu gaucacugca aacccgguaa ucaccgaaag cacagaaaac ucaaaaauga     1740

ugcucgagcu cgacccuccc uucggagaca gcuacaucgu caucggaguc ggagaaaaga     1800

aaauaacuca ucacuggcac cgcagcggau caaccaucgg caaagccuuc gaagcuacag     1860

ucagaggcgc aaagagaaug gcgguacuag gcgacacagc augggacuuc ggaucagugg     1920

gaggcgcacu gaacagccuc ggaaagggga uacaccaaau cuucggcgcc gcauucaaaa     1980

gccucuucgg cggcaugagc ugguuuuccc agauacuaau cggaacgcuc cuaauguggc     2040

uagggcuaaa caccaaaaac ggcagcauaa gccugaugug ccucgcgcuc ggaggcgucc     2100

ucaucuuccu aucaaccgca gucucggcag auguaggcug cucagucgac uucagcaaau     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  7192
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7192
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggggg       60

ccgacacuuc cguaggcauc gucggacuac uccugaccac ggccauggcc gccgaaguaa      120

cccgccgcgg cagcgcauac uacauguacc ucgaccgcaa cgacgccggg gaagcuauau      180

cguucccaac aacccucgga augaacaaau gcuacauaca aaucauggac cucggccaca      240

ugugcgacgc aaccaugagc uacgaauguc caaugcugga cgaaggggua gaaccagacg      300

acguggacug cuggugcaac accacaagca ccugggucgu cuacggaacc ugccaccaca      360

agaaaggaga ggcccgacga agucgccgcg cagucacccu cccaagccac ucuacacgaa      420

aacuucaaac ccggucacaa acauggcucg aaucaagaga auacaccaaa caccucaucc      480

gagucgaaaa cuggaucuuc agaaaccccg gauucgcccu cgcagcggcc gccauagcgu      540

ggcuacuagg cucaagcacc ucacaaaaag ucaucuaccu ggucaugauc cuccuaauag      600

cacccgccua cagcauccgc ugcaucggcg ucucaaacag agacuucgua gaaggaauga      660

gcggaggaac auggguagac auagugcucg aacauggagg cugcguaacc gucauggccc      720

aagacaaacc aaccgucgac auagaacugg ucacuacaac cguaucuaac auggccgagg      780

uaagaucaua cugcuacgaa gccuccaucu ccgacauggc cagugacagc cgaugcccaa      840

cgcaaggcga agccuaucuc gacaaacaau ccgacaccca auaugucugc aagcgcacac      900

ucguagaccg cggcugggga aacggcugcg gccucuucgg caaaggcagc cuaguaaccu      960

gcgcaaaauu cgcaugcucc aagaaaauga caggaaaauc cauccaaccc gaaaaccucg     1020

aauaccgcau caugcucucc gugcacggca gccaacacuc cggaaugauc guaaacgaca     1080

ccggacacga aacagacgaa aaccgcgcca aagucgaaau aacaccaaac uccccacgcg     1140

cggaagccac acuaggcggc uucggcuccc uaggccucga cugcgaaccc agaacaggac     1200

ucgacuucag cgaccucuau uaccuaacca ugaacaacaa acauugguua guacacaagg     1260

aaugguuuca cgacaucccc cuccccuggc acgcaggcgc agauacagga acaccacacu     1320

ggaacaacaa agaagcccuc guagaauuua aagacgccca cgcuaaacgg caaacaguag     1380

uaguccuagg cagccaagag ggggccguac acaccgcacu cgcaggcgcc cucgaagcug     1440

aaauggaugg cgccaaaggc aggcucucau cgggucaccu caaaugccga cuaaaaaugg     1500

acaaacuacg ucuaaaaggg gucucuuacu cacucuguac ggcggccuuc accuucacca     1560

aaauaccagc agagacacua cacggaaccg ucacagucga gguccaauac gccggcacag     1620

acggaccaug caaagucccu gcacaaaugg ccgucgacau gcaaacccuc acaccaguag     1680

gacgccucau cacggccaac cccgucauca cagaaagcac cgaaaacucc aaaaugaugc     1740

ucgaacuaga ucccccguuc ggagacagcu acaucguaau aggcgucgga gagaagaaaa     1800

uaacacacca cuggcaccga uccggaucca caauaggcaa agccuucgaa gcaaccguca     1860

gaggcgcaaa acgaauggcc guacugggag acacagccug ggacuucgga agcgugggcg     1920

gagcacucaa cagccuuggc aaaggaaucc accaaaucuu cggcgcagca uucaaaucac     1980

ucuucggagg cauguccugg uucucacaaa uacugauagg gacacuccug augugguuag     2040

gucucaacac caaaaacggc agcauuucuc ucaugugccu cgcccucgga ggaguacuca     2100

ucuuccucuc cacagcgguc agcgcagacg ucggaugcuc cgucgacuuu ucgaaaugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  7193
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7193
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acaccagcgu cggaaucguc gggcuccuau ugaccaccgc cauggccgcc gaagucaccc      120

gccgcggaag cgccuacuac auguaccuag accgaaacga cgcaggcgaa gccaucagcu      180

uccccacaac acuaggcaug aacaaaugcu acauucaaau cauggaccua ggccacaugu      240

gcgacgcaac caugucauac gaguguccca ugcuagacga aggaguagaa ccagacgaug      300

uagacugcug gugcaacacc accuccacau gggucgucua cggaaccugc caccacaaaa      360

agggcgaggc acgacgauca cgccgcgccg ucacucuacc aucacacuca acucgaaaac      420

uacaaacccg cucccaaaca ugguuagaaa gccgcgagua uacgaaacac cuaauccgag      480

uagaaaauug gauauuccgg aacccaggcu ucgcacuggc agccgccgca aucgcauggc      540

uucugggauc cucaacguca caaaaaguca ucuaccucgu caugauacug cucauagcac      600

cagcauauuc cauccgcugu aucgggguau ccaacaggga cuucguugaa ggcaugucag      660

gagggacaug ggucgacauc guccucgaac acggaggcug cgucacaguc auggcacaag      720

acaaacccac cguagacauc gagcucguca ccacaacagu cagcaacaug gccgaagucc      780

gcagcuacug cuacgaagca ucaaucagcg auauggccag cgacucaagg ugcccuacuc      840

aaggcgaggc uuaccuagau aaacaaagcg acacccaaua cgucugcaaa cgcacccuag      900

uagaccgagg auggggaaac ggaugcggcc ucuucggcaa aggcucccuc gugacaugcg      960

caaaauucgc augcagcaag aaaaugacag gcaaaagcau acaaccagaa aaccucgaau     1020

acagaaucau gcugagcguc cacgggucac aacacucagg caugauagug aacgacaccg     1080

gccacgaaac cgacgaaaac cgcgcaaaag uagaaauaac cccaaacagc ccacgagccg     1140

aagccacccu aggcggcuuu ggaagccucg gccuagacug cgagccccgc accggucuag     1200

acuucagcga ccucuacuac cugaccauga acaacaaaca uuggcucgug cacaaagagu     1260

gguuccacga cauaccccua ccuuggcacg caggagcaga caccggcaca ccccacugga     1320

acaacaagga ggcacuggua gaguucaagg acgcccacgc aaaacgacaa acaguagucg     1380

ugcuaggauc ucaagaaggc gccguccaca cagccuuagc cggggcacuc gaagcagaga     1440

uggacggagc caaaggacga cuauccuccg gccaucucaa augccgccuc aaaauggaca     1500

agcuccgacu aaaaggcgua agcuacagcc uuugcacggc agcauucacg uucacgaaaa     1560

uucccgccga aacacuccac ggcacaguaa ccgucgaagu acaauacgcc ggcacagaug     1620

ggcccugcaa gguccccgcc caaauggcag uugacaugca aacccucacc cccguaggcc     1680

ggcugaucac cgccaaccca gucaucaccg aauccaccga gaacagcaaa augaugcucg     1740

aacucgaucc cccuuucgga gacuccuaca uagucaucgg aguaggagaa aagaaaauca     1800

cccaccacug gcaccgcagc ggcuccacca uaggaaaagc auucgaagcu accguccgcg     1860

gggccaaacg cauggccgua cucggcgaca cggcguggga cuucggcucg guuggaggag     1920

cccuaaauag ccucggcaaa ggaauccacc aaauauucgg agcggcuuuc aaaucccuuu     1980

ucgguggcau gucaugguuu agccaaauuc ucaucggaac acugcucaug uggcugggcu     2040

uaaacaccaa aaauggauca aucucgcuca ugugccuagc acuaggaggg guccuaauau     2100

uccugucaac cgcagugagc gccgacguag gcugcagcgu cgacuucuca aaaugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  7194
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7194
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

caucaguugg caucgucggg cugcuacuca ccacggccau ggccgcagaa gugacaagaa      120

gaggaagcgc auacuacaug uaucuggauc gcaacgacgc cggcgaagcg aucagcuucc      180

caacgacccu cggaaugaac aaaugcuaca uacagaucau ggaccuagga cacaugugcg      240

acgcgaccau guccuacgag ugcccaaugc uagacgaagg cgucgagcca gacgacgucg      300

acugcuggug uaauacgacc ucaaccuggg uagucuacgg uacaugccac cacaagaaag      360

gcgaagcacg aagaucaaga cgagccguca cccuacccuc acacagcacu agaaaacucc      420

aaacccgaag ucaaacaugg cuagaaaguc gagaauacac gaaacaccug auccgcguag      480

aaaacuggau auuccgaaac ccaggcuucg cgcucgccgc agccgccaua gccuggcucc      540

ucggcagcag caccagucag aaggucauau aucucgugau gauccuccuc aucgccccug      600

ccuacucaau aagaugcauc ggcguaagca accgggacuu cguggaagga augucuggag      660

gcaccugggu agacauaguc cucgaacacg gaggcugcgu aacuguaaug gcccaagaca      720

aaccuacagu cgacauugaa cuggucacca ccaccguauc caacauggcc gaagugcgca      780

gcuacugcua cgaagccagc aucucagaca uggcgaguga cagccgaugc cccacucaag      840

gcgaagccua ccuagacaaa caaagcgaca cacaauacgu cugcaaacga acacucgucg      900

aucguggaug ggguaacgga ugcggccucu ucggcaaagg uucccuaguc acuugcgcaa      960

aauucgccug cagcaaaaag augacaggaa agagcauaca acccgaaaac cuagaauacc     1020

gcaucaugcu gagcguccac ggcagccagc acagcggaau gauaguaaac gacacaggac     1080

acgaaaccga cgaaaauagg gcgaaagucg aaaucacccc caacagcccc agagcggaag     1140

caacacuagg uggcuucggg agccucggau uagacugcga gccccgaaca ggccucgacu     1200

ucagcgaccu auacuaccua acaaugaaca acaaacacug guuaguccac aaagaguggu     1260

uccaugacau cccccuccca uggcacgccg gcgccgacac cggcacaccc cacuggaaca     1320

acaaggaggc cuuagucgag uucaaagacg cccacgcaaa acgacaaaca gugguagucc     1380

uaggaagcca ggaaggagcc guccacacgg cccucgcagg agcacucgag gccgaaaugg     1440

acggcgcaaa aggccgccug agcucaggac accucaaaug ccgccuaaaa auggacaaac     1500

uaagacucaa aggaguaagc uacucccuuu gcacagcagc cuucacauuc acgaagaucc     1560

cagcagaaac acugcauggc acagugacag uagaaguaca auacgccggc accgacggac     1620

caugcaaagu gcccgcccag auggccguag auaugcagac ucuaacuccc guaggcagac     1680

uaaucaccgc aaaccccguc aucaccgaga gcacagaaaa cagcaaaaug augcuagagc     1740

ucgauccccc auucggagac aguuacauag uaaucggagu aggcgagaag aaaaucaccc     1800

accacuggca ccgcagugga uccacuauag gcaaagccuu ugaagcaaca guccgcggcg     1860

caaaaaggau ggcaguccua ggagacaccg caugggauuu cggcagcgua ggcggagcac     1920

uaaacagccu cggcaaaggc auacaccaaa uauucggagc cgccuucaag agccuauucg     1980

gcggcauguc cugguucagc caaauacuaa ucggcacccu ccucaugugg cucggacuga     2040

acaccaaaaa cgggucaauc agccucaugu gccuggcacu gggaggggua cucauauucc     2100

uaagcaccgc aguaucagcc gacguagguu gcagcguaga cuuuagcaaa ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  7195
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7195
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

cagucggcau aguaggacug cuccugacaa cagcgauggc cgccgaggua acucgaagag      120

gaagcgcgua cuacauguac cucgaccgaa acgacgcggg cgaagcaauc agcuucccga      180

ccacccuagg caugaacaaa ugcuacaucc aaauaaugga ucugggacac augugcgacg      240

caaccauguc auacgaaugu cccaugcucg acgaaggagu agaacccgac gacguggauu      300

gcuggugcaa cacaaccagc accuggguag ucuauggaac augccaccac aagaaaggcg      360

aagcccgacg auccagacgu gccgucacgc uacccagcca cagcacacgc aaacuccaaa      420

ccagaagcca aacauggcuc gaaagccgag aauacaccaa acaccucaua agagucgaaa      480

acuggauauu ccgcaacccc ggguucgcac uagcugcagc agccaucgca uggcuccuag      540

gcuccagcac aagccaaaaa gucauauacc uugucaugau ccuacucaua gcaccggccu      600

acuccauacg cugcauaggc gucuccaacc gagacuucgu cgaaggaaug agcggaggca      660

ccuggguaga uaucguccug gagcacggcg gaugcguaac cgucauggcg caagacaagc      720

caaccguaga caucgagcuc guaaccacca cgguuagcaa cauggccgaa guccgcuccu      780

acugcuacga agcaagcaua agcgacaugg caagugacag cagaugcccc acucaaggcg      840

aagcguaccu cgacaaacaa agcgacacac aauacguaug caaacgaacc cucguagaca      900

gaggcugggg caacggcugc ggccuauucg gaaagggcuc ccucguaacc ugcgcgaagu      960

ucgccugcuc aaagaaaaug acaggcaaau cgauacaacc ggaaaaccuc gaauacagaa     1020

uaaugcuguc cguccacgga ucacaacaca gcggaaugau agugaaugac acaggacaug     1080

aaaccgacga gaaccgagca aaagucgaaa uaacaccaaa cuccccacgg gccgaagcca     1140

cccucggagg uuuuggcagc uuaggccucg acugcgagcc acgaacgggc cucgacuucu     1200

cagaccucua cuaccuaacg augaacaaca aacacuggcu uguccauaaa gaaugguucc     1260

acgacauacc ccuccccugg cacgccggag ccgacaccgg cacaccccac uggaacaaca     1320

aagaggcgcu aguagaguuc aaagacgccc acgccaagag acaaaccgug guaguacuag     1380

ggagccaaga aggcgcggua cacacagcac uagccggagc ccucgaagcc gaaauggaug     1440

gagcaaaagg gcgacucagc uccggccacc ucaaaugccg ccuaaaaaug gacaaacuaa     1500

gacugaaggg cguaagcuac agccuaugca cagccgcauu caccuucacc aaaauacccg     1560

ccgaaacccu acacggaaca gucaccgucg aaguccaaua cgccgguacc gacggaccgu     1620

gcaagguacc ggcacaaaug gcaguagaca ugcaaacucu aacccccgua ggcagacuca     1680

ucacggccaa ccccguaaua accgaaucua cagaaaacag uaagaugaug cucgaacucg     1740

acccacccuu uggcgacucc uacaucguca uaggagucgg agaaaagaaa aucacccacc     1800

acuggcacag gaguggcagc acaaucggua aagccuucga agccacagua cgcggggcaa     1860

aacgcauggc gguccucggc gacacagcau gggacuucgg gagcguaggc ggcgcgcuaa     1920

acagccucgg caaaggcauc caccagaucu ucggcgcggc auucaaauca cuauuuggag     1980

ggaugagcug guucucucaa auccucauag gaaccuugcu gauguggcug ggccuaaaca     2040

cgaaaaacgg gagcaucagc cucaugugcc uugcccuagg uggcguccua aucuuccuua     2100

gcacagccgu cucagccgac gucggaugca gcguggacuu caguaaauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  7196
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7196
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucgguau aguaggccua cuacuaacaa cggccauggc agccgccgug acacgcagag      120

gaucugccua cuacauguac cuagaccgca acgacgccgg cgaagcgauc uccuucccua      180

cuacccuggg caugaacaaa ugcuacaucc aaaucaugga ccuaggacac augugcgaug      240

ccacaauguc auacgaaugc ccuaugcugg acgagggagu cgaacccgac gacguagauu      300

guuggugcaa caccacaagc acauggguag uauauggaac augccaccac aaaaagggcg      360

aagcgcgcag aagccgccgc gccgucaccc uacccagcca cuccacaaga aaacugcaaa      420

cgagaagcca aaccuggcuc gaaucccgcg aauacaccaa acaccucauc cgcgucgaaa      480

acuggaucuu ccgaaacccc ggcuucgcgc uagccgcggc cgcuaucgcg uggcuccugg      540

gaucaaguac cagccaaaaa guaaucuacc uaguaaugau ccuccucauc gcaccagccu      600

acuccauccg augcauuggc guaagcaaca gagacuucgu cgagggcaug aguggcggca      660

ccuggguaga caucgugcua gaacacggag gcugcgucac cguaauggcc caagacaaac      720

ccacagucga cauagaacuc guaacaacaa cgguuucaaa cauggcagag gugagaucgu      780

auugcuacga agccagcauc agcgauaugg ccagcgacag ccgcugcccg acacaaggag      840

aagcguaccu ggacaaacaa agcgacaccc aauacgucug caagcgaaca cucguagacc      900

gcgggugggg caacggcugc ggacuauucg gaaaaggaag ccuuguaacc ugcgcaaagu      960

ucgccugcag caagaaaaug acaggcaaaa gcauccaacc agaaaaccuc gaauaccgga     1020

ucaugcucag cguccauggc ucacaacaca gcggcaugau agucaacgac acgggccacg     1080

aaacagacga aaaccgagca aaaguagaaa uaacccccaa cuccccaaga gcggaagcca     1140

cccuaggcgg auucggaucc cucggacucg acugcgaacc acgaaccgga cucgacuucu     1200

cagaccucua cuaccucacc augaacaaca aacacuggcu cguccacaaa gaaugguucc     1260

acgacauacc acucccaugg cacgccggcg ccgacacagg cacaccacac uggaacaaca     1320

aagaggcccu ugucgaguuc aaagacgcac acgcaaaacg ccaaaccguc guaguccucg     1380

gaucccaaga aggagcgguc cacaccgccc ucgcaggcgc ccuagaagcc gaaauggacg     1440

gagcgaaagg acgccuauca ucggggcacc ucaagugcag acucaaaaug gacaaacucc     1500

gacuaaaagg cgucagcuac ucacucugca ccgccgcauu cacguucacc aaaauccccg     1560

ccgaaacccu acacggaaca gugacaguag aagugcagua cgcaggaaca gacggaccau     1620

gcaaaguccc cgcccagaug gcaguagaca ugcaaacacu cacaccagug ggacgccuaa     1680

ucaccgccaa ccccguaauc acagaaagca ccgaaaacag caaaaugaug cuagaacucg     1740

acccacccuu cggggacucc uacaucguaa uaggagucgg ggaaaagaaa aucacgcacc     1800

acuggcaccg aagcgguucc acaaucggca aagccuucga agcaacagua cgcggcgcaa     1860

aacggauggc gguccucgga gacaccgcau gggacuucgg aagcgucgga ggcgcccuaa     1920

acagucuagg caaaggaaua caccaaauau ucggugcggc cuucaaaagu cuauucggcg     1980

gcaugagcug guucagccaa auccucauag gaacacucuu aauguggcuc ggccuaaaua     2040

cuaaaaacgg cucaauuagc cuaaugugcu uagcacuagg aggcgugcuc aucuuccugu     2100

caaccgccgu cuccgccgac guaggcugca gcguagacuu uagcaaauga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  7197
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7197
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gcgcagacac gagcguaggc aucgucggcc ugcuuuuaac cacagcaaug gcugcagccg      120

uaacccgacg aggaagcgca uauuacaugu accuagacag aaaugacgca ggagaagcaa      180

uaucguuccc cacaacacua ggaaugaaua agugcuacau ccaaaucaug gaccucggac      240

acaugugcga cgccacgaug ucguacgaau gccccaugcu agacgaagga gucgaaccag      300

acgacgugga uugcuggugu aacacaaccu caaccugggu gguauacggc acgugccacc      360

acaagaaagg cgaagcaaga cgaucaagac gagcaguaac ucuccccucc cacagcacca      420

gaaaacucca gacaagaucc caaacauggc uagaaucacg agaauacaca aaacaccuga      480

uaagagucga aaacuggaua uucagaaacc ccggauucgc acucgccgca gcagccauag      540

cauggcucuu aggcagcuca acaagccaaa aaguaaucua ccuagucaug auccuacuaa      600

ucgcgcccgc guacucgauc cgcugcaucg gcguaucgaa cagagacuuc gucgaaggca      660

uguccggagg gacguggguc gacauagugc uagaacacgg cggaugcguc acagucaugg      720

cacaagacaa accgacgguc gacauagagc uaguaaccac cacaguaagc aacauggcag      780

aagucagaag uuacugcuac gaagcaucca uaucagauau ggcaucagac uccagaugcc      840

cgacccaggg agaggccuac cuagacaaac aaucagauac ccaauacguc ugcaaacgaa      900

cccugguaga ccgcggcugg ggaaacggcu gcggccuguu cggcaagggg agcuuaguaa      960

ccugcgcaaa auucgcaugc agcaagaaaa ugaccggaaa aagcauccaa cccgaaaacc     1020

ucgaauaucg cauaaugcuc agcguccacg gcagccaaca cagcggaaug auagucaacg     1080

acacaggcca cgaaacagac gaaaacaggg caaaagucga aaucacacca aauagcccaa     1140

gggccgaagc aacacucggg ggcuucgggu cacuuggccu agacugcgaa cccagaacgg     1200

gacuagacuu cagcgaccug uacuaccuca ccaugaacaa caaacacugg cuaguacaca     1260

aagaaugguu ccacgacaua ccccucccau ggcacgcagg agccgacacc gggacgccac     1320

acuggaacaa caaggaagca cucgucgagu ucaaagacgc ccacgcaaag cgccaaacag     1380

uagucguacu gggcucccaa gaaggggcag uacacacggc acucgcaggc gcacucgaag     1440

cagaaaugga cggcgccaaa ggccgccucu caagcggaca ccucaaaugc cgccucaaga     1500

uggacaagcu ccgacucaaa ggcguaagcu acagccuuug cacagcagca uucacauuca     1560

caaaaauacc cgcagaaaca cuccacggca cagucacagu cgaagugcaa uacgcaggaa     1620

ccgacggccc augcaaggua ccagcacaaa uggcagucga caugcaaacg cuaaccccag     1680

ucggccgccu cauaaccgca aacccaguaa uaacugagag cacagaaaac agcaagauga     1740

ugcucgagcu cgacccaccc uucggcgaua gcuacauagu gauaggaguc ggcgagaaaa     1800

agaucacgca ccauuggcac agaagcggaa gcacaauagg aaaagcguuc gaagcaacag     1860

ucagaggcgc aaaacgcaug gccguccucg gcgacacagc augggacuuc ggcucaguag     1920

gaggggcacu caauagccua ggcaaaggaa uacaccagau auucggagcg gcauucaaaa     1980

gccuguucgg cgguaugagc ugguucagcc agauacuaau uggaacccua cuaauguggc     2040

uaggccuaaa caccaaaaac ggaagcauca gccuaaugug ccuagcacuu ggcggaguac     2100

ucauauuccu cagcacagca gucuccgccg acguaggaug cagcgucgac uucagcaaau     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  7198
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7198
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

ggcucucauc acgcaaacgc cgcagucacg acguccuaac cguccaauuu cuaauccuag      120

gaaugcuccu aaugaccgga ggggucaccc gcagaggcuc ggccuacuac auguaccucg      180

acaggaacga cgccggcgaa gcaauuagcu uccccacaac ccuugguaug aacaaaugcu      240

acauacaaau aauggaccua ggacauaugu gcgacgcaac gauguccuac gaguguccca      300

uguuggacga aggcguagaa ccagacgacg uggacugcug gugcaacacc acaagcacau      360

ggguuguaua cggcacaugc caccacaaga aaggagaagc ccgccgauca cgacgugccg      420

ucacccugcc cagccacagc acgcgcaaac uacaaacucg cucgcaaacc uggcuggaaa      480

gccgcgaaua cacaaaacac cucauccgag ucgagaacug gauauuccgc aaccccggcu      540

ucgcacucgc ggcagccgca auagcauggc uucuaggguc aagcaccagc caaaaaguca      600

ucuaccucgu aaugauccuc cugaucgccc ccgccuacuc caucagaugc aucggaguau      660

caaacagaga uuucguagaa ggcaugucag guggaaccug gguagacauc guacuagagc      720

acggaggcug cgucaccguc auggcacagg auaaacccac agucgacauc gaacugguca      780

cgaccacugu cucaaacaug gccgaagugc guagcuacug uuacgaagcc agcaucagcg      840

acauggcgag cgacagucga ugccccaccc aaggggaagc auaccucgac aaacaaagcg      900

acacacaaua cguaugcaag cgaacacucg uagaccgcgg cugggggaac ggaugcggac      960

ucuucggaaa aggcagccua gugaccugcg ccaaauucgc uuguucaaag aaaaugaccg     1020

gcaaaagcau acaaccggaa aaccucgaau acagaauaau gcucuccgug cacggaucuc     1080

agcacagcgg caugauaguc aacgacaccg gccacgagac cgacgaaaac agggcgaaag     1140

ucgaaauaac accgaacagc ccccgagccg aggcgacacu cggcggcuuc gggucccucg     1200

gccuagauug cgaaccccgc acaggccucg acuucagcga ccucuauuau cuaacaauga     1260

acaacaaaca cuggcuaguc cacaaagaau gguuccacga cauuccacuc cccuggcacg     1320

cgggagcgga cacgggaaca ccacacugga acaacaaaga agcacucgua gaguucaaag     1380

acgcacacgc caaacgacaa accguaguag uacuaggaag ccaagaaggc gccgugcaca     1440

cagcacuagc aggggcccua gaagccgaaa uggacggcgc caaaggccga cuaagcagug     1500

gacaccuaaa augccgccua aaaauggaca aacuacgccu aaaaggaguc agcuauagcc     1560

uaugcacagc cgcauucacg uucacaaaaa uucccgcgga aacgcuacac ggaacuguaa     1620

ccgucgaagu acaauacgcc ggcaccgacg gaccgugcaa ggucccugca caaauggcug     1680

uagacaugca aacacucaca ccggucggcc gucuaauaac agccaacccc guaaucaccg     1740

aauccaccga aaacucaaaa augaugcugg aacucgaccc acccuucgga gacaguuaca     1800

uagucaucgg agucggagag aaaaagauca cucaccacug gcacagauca ggcucaacga     1860

ucggaaaagc cuucgaagca acaguccgcg gggcaaaaag aauggccguc cuaggagaca     1920

cagccuggga uuucgguagc guaggaggag cccucaacuc ccuaggaaag ggcauacacc     1980

aaauauucgg agccgccuuc aaaagccucu ucggaggcau gucgugguuc ucgcaaauac     2040

uaaucggcac ccuccugaug uggcuagggc uaaacaccaa aaacggaucg aucucucuca     2100

ugugccuagc ccucgguggc gugcuaauau uccucuccac cgccgucagc gcggauguag     2160

gaugcagcgu agacuucagc aagugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  7199
<211>  2515
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7199
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gacucagcuc ccgcaaacga cgcucacacg acguacuaac cgugcaauuc cucauccuug      120

ggaugcuacu caugacaggc gcggugaccc gacgcggcag cgcauacuac auguaccucg      180

accgaaacga cgcgggcgaa gcaaucagcu uccccacaac acucggcaug aacaaaugcu      240

acauacaaau aauggaccuc ggacacaugu gcgacgccac caugucguac gagugcccaa      300

ugcuagacga aggcguagaa cccgacgacg ucgacugcug gugcaauacc accucaacau      360

ggguagucua cggcacaugc caccacaaga aaggagaagc ccgccgcucu agacgcgccg      420

ucacucuccc cagucacuca acccgaaaac uccaaacgcg cucccaaaca uggcucgagu      480

cccgcgaaua cacaaaacac cucauccgag uagaaaacug gaucuuccga aaccccggau      540

ucgcacucgc cgcagcagcc auagcauggc uccugggauc aagcacaucc cagaaaguaa      600

ucuaccucgu caugauccua cuaaucgcuc ccgcguacag cauacgaugc auaggcguca      660

gcaacagaga cuucguagaa ggaaugucgg gcggcacaug gguagacauc gugcucgagc      720

acggcggaug cguaaccgua auggcccaag acaaacccac aguagacauc gagcucguaa      780

cuaccacagu caguaacaug gccgaagucc gcagcuacug uuacgaggcc ucaauuucag      840

acauggccuc cgacagucgc ugcccaacac aaggcgaagc guaccuggac aaacaauccg      900

acacccaaua cguaugcaaa cgcacccucg uugacagggg guggggaaau ggaugcggau      960

uauucgguaa agggucccua guaaccugcg ccaaauucgc augcucaaag aaaaugaccg     1020

gcaaaucaau acaaccagag aaccuagaau accgcauaau gcucagcguc cacgggagcc     1080

aacauuccgg aaugaucgua aacgacaccg gacacgaaac cgaugagaau cgcgcaaaag     1140

uagaaaucac accaaacucc ccacgggcug aggcgacacu aggcggcuuc ggcagccuag     1200

gccuagacug cgaaccccgc accgggcucg acuucucgga ccuguacuac cucacgauga     1260

acaacaaaca cuggcucgua cacaaagaau gguuccacga uaucccccuc ccauggcacg     1320

ccggcgccga caccggaaca ccccacugga acaacaaaga agcccucgua gaguucaaag     1380

acgcccacgc caaaagacaa acagugguug uacuaggguc acaggaaggc gccguacaca     1440

cagcacucgc cggagcccuc gaagccgaaa uggacggggc aaaagguaga cuaaguagcg     1500

gccaccucaa gugccgacuc aaaauggaca agcugcgacu gaaagggguc aguuacuccc     1560

uaugcaccgc ugcauucacc uucaccaaga ucccggccga aacccuacac ggaacaguca     1620

ccgucgaagu gcaguacgcc ggcacagacg gccccugcaa aguacccgcg caaauggccg     1680

ucgacaugca aacacucacg cccgucggac ggcucaucac agccaacccc guaaucacgg     1740

aaagcacaga gaacagcaaa augaugcuag aacuagaccc acccuucgga gacuccuaca     1800

uaguaaucgg cguaggcgag aagaaaauua cacaccacug gcacaggucc ggcucaacga     1860

uugguaaagc cuucgaagca acaguccgcg gugccaagcg cauggcaguc cuaggcgaca     1920

cagcauggga cuucggcuca gucggcggcg cacucaacag ucugggaaag ggcauacauc     1980

agauauucgg cgcagccuuc aagucgcuau ucggagggau gagcugguuc agccagaucc     2040

ucaucggaac acugcucaug uggcuaggac uaaacaccaa aaacgguagc aucagccuca     2100

ugugccuagc acuaggcgga guccuaauau uccuuagcac ggcagucagc gccgacgugg     2160

gcugcagugu cgacuucucu aaaugaggac uagugcauca cauuuaaaag caucucagcc     2220

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2280

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2340

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2460

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2515


<210>  7200
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7200
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc gcgcaaagaa aagaaacgac gcggagccga caccucgguc ggaauagucg      120

gccuccuacu gacaaccgcc auggcagccg aggucacccg acgaggcucc gccuacuaca      180

uguaccucga ccgcaacgac gccggagagg ccauuuccuu ccccaccacc cucggcauga      240

acaaaugcua cauccaaauu auggaccuag gacacaugug cgacgcaaca augagcuacg      300

aaugcccaau gcuagacgaa gguguagaac ccgacgacgu agacugcugg ugcaacacaa      360

cauccacaug ggucguguac gguacaugcc aucacaagaa aggcgaagca cgccgcagcc      420

gacgcgccgu aacccuccca ucccacagca caaggaaacu ucaaacacgc agccagacgu      480

ggcucgaaag cagagaauac acaaaacacc ucauacgagu agaaaacugg auauuucgca      540

acccgggcuu ugcccucgcc gccgcggcca uagcauggcu auuaggcagc aguacguccc      600

aaaaaguaau uuaccuaguc augauacuac uaauagcacc agccuacucc auaaggugca      660

ucggaguguc aaaccgcgac uucgucgaag gaaugagcgg aggaacaugg gucgacaucg      720

uccucgaaca cggcgguugc gucacaguca uggcccaaga caaacccaca guagacaucg      780

aacucgucac gacaacagua uccaauaugg ccgaaguacg auccuacugc uacgaggcaa      840

gcauaucaga cauggccucc gacucaagau gcccaaccca aggagaagcc uaccucgaca      900

aacaguccga cacucaauac gugugcaaac ggacacuugu cgaccgcggg uggggcaacg      960

gauguggccu auucggcaaa ggcagucugg uaaccugcgc aaaauuugca ugcuccaaga     1020

aaaugacagg gaaauccaua caacccgaaa accucgaaua cagaaucaug cucucagucc     1080

acgggucaca gcacagcggc augauaguaa acgacaccgg ccacgagaca gacgaaaacc     1140

gcgccaaagu agaaauaacc ccaaacagcc cccgggccga ggccacccug ggcggguucg     1200

gcagccucgg ucucgacugc gaaccccgaa caggucuaga uuucucagac cuauacuacc     1260

uaaccaugaa caacaaacau uggcucguac auaaagagug guuccacgac aucccacucc     1320

cauggcacgc aggcgcggac accgguaccc cacacuggaa caacaaagaa gcgcuagucg     1380

aguucaaaga cgcacacgca aaaagacaaa cagucguagu ccuaggcucc caggaaggag     1440

ccguccacac ggcacucgcc ggagcacucg aggcagagau ggacggcgcc aaaggccgcc     1500

ucagcucagg acaccuaaaa ugccgccuca aaauggacaa acucagacuc aaagggguau     1560

cauacagccu augcacagcc gcauuuacgu ucaccaaaau accagccgag acacuccacg     1620

gcaccguaac agucgagguc caauacgcag gaacagacgg ccccugcaaa gucccagccc     1680

aaauggccgu cgacaugcaa acacucacac ccgugggccg ucuaauaacc gcaaaccccg     1740

uuauaacaga auccacagaa aacuccaaaa ugaugcugga acucgauccc ccauucgggg     1800

acagcuacau cguaaucggc guaggcgaaa agaaaaucac ccaccacugg caccgcuccg     1860

gaagcacaau aggcaaagca uucgaagcaa ccguccgcgg agcgaaaaga auggcuguac     1920

ucggagacac cgcgugggac uucggcagcg ugggcggcgc acucaacucc cuaggaaaag     1980

gcauccacca gauauucggc gcagccuuca aaucccuuuu cggcggcaug uccugguucu     2040

cccaaauccu uauaggaacc cuucuaaugu ggcucggccu caacaccaaa aacggcucca     2100

uaucccucau gugccucgcc cucggcggcg uacuaaucuu ccuaagcaca gccgucagcg     2160

ccgacgucgg augcugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7201
<211>  2503
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7201
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

ucaacgcccg aaaagaaaag aaacgacgag gcgccgacac aagcguaggc aucguaggac      120

uccugcucac uacagccaug gcagcagaag ugacccgccg cggauccgcc uacuacaugu      180

accucgaccg caacgacgcc ggagaggcaa uaucauuccc caccacgcua ggaaugaaca      240

aaugcuacau acaaauaaug gaucucggac acaugugcga cgccaccaug agcuacgaau      300

guccaaugcu agacgaggga guugaacccg augacguaga cugcuggugc aacaccacau      360

cgaccugggu cgucuacggu accugccacc acaagaaagg cgaagcccga cgcucacgcc      420

gagccgucac ucuacccagc cacuccacaa gaaaacucca aacacgcagc caaacauggc      480

uagaaucccg cgaauacaca aaacaccuaa uacgaguaga gaacuggauc uucagaaacc      540

ccggcuucgc gcucgcagcg gccgccaucg cauggcucuu aggcuccagc accagccaga      600

aaguuaucua ccucguaaug auccuccuca ucgccccagc cuacuccauc cgaugcauug      660

gaguaagcaa cagggacuuc guagaaggaa ugagcggcgg aacauggguc gacauaguac      720

ucgaacacgg aggcugcgua accguaaugg cacaagacaa acccaccgug gacauagaac      780

ucgucacaac caccguuucc aacauggccg aagugagaag cuacuguuac gaagccagca      840

ucucagacau ggcuuccgau agcagaugcc ccacccaagg agaggcauac cucgauaaac      900

aaucagacac ccaauacguc ugcaaaagaa cacucguaga ccgcgguugg ggcaacggcu      960

gcggacucuu uggcaaagga ucccuaguua ccugcgcaaa auucgccugc agcaaaaaga     1020

ugacgggcaa gucaauacag cccgaaaauc uggaauaccg gaucaugcua ucgguccacg     1080

gcagccaaca cagcggcaug aucgugaacg acaccggcca cgaaaccgac gaaaacagag     1140

caaaagucga gauaacccca aacucacccc gagcagaagc cacccuaggc ggauucggca     1200

gccugggccu agacugcgaa cccagaaccg gccuagacuu cuccgaccug uacuaccuca     1260

caaugaacaa caaacauugg uuaguacaca aagaaugguu ccacgacaua ccucuaccau     1320

ggcacgccgg cgcagacacc gggaccccgc acuggaacaa caaggaagca cugguagagu     1380

ucaaggacgc acacgcaaaa cgccaaacgg ucgucgugcu cggcagccaa gaaggagccg     1440

uccacaccgc gcuagcagga gcucucgagg cggaaaugga cggcgccaaa ggcaggcucu     1500

ccucagggca ccucaaaugc cgccucaaaa uggacaaacu ccgacucaaa gguguguccu     1560

acucucucug cacagcagcc uucacauuca ccaagauccc ugccgaaaca cuccacggga     1620

cagucacggu agaaguccaa uacgccggua cagacggacc gugcaaaguc ccagcccaaa     1680

uggccguaga caugcagaca cuuaccccag ucggccgccu caucaccgcc aaccccguca     1740

ucacagaaag caccgaaaac ucaaaaauga ugcuggaacu cgacccaccc uucggugaca     1800

gcuacaucgu aaucggagua ggagagaaga aaauaacaca ccacuggcau cgcagcggcu     1860

ccaccaucgg caaagcauuc gaggcuacag uaagaggagc caaacgaaug gccguccuag     1920

gagacaccgc augggacuuc gggagcgucg gcggcgcacu aaacagccuc ggaaaaggga     1980

uacaucaaau cuucggcgcc gccuuuaaau cauuauucgg cggcaugagc ugguucagcc     2040

aaauauuaau cggaacgcua cucauguggc uagggcucaa caccaaaaac ggauccaucu     2100

cacuaaugug ccuggcucuc ggaggcgucc ucaucuuccu uucaacagca guaagcgccg     2160

acguaggcug cugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     2220

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     2280

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     2340

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2460

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2503


<210>  7202
<211>  2500
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7202
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccgcaa agagaagaaa cgcagaggcg ccgacacgag cguaggaauc gucggccuac      120

uacucacuac agccauggcg gccgagguca cacgcagagg gucagccuac uacauguauc      180

ucgacaggaa cgacgccgga gaagcgaucu cauuccccac cacccucgga augaacaaau      240

gcuacaucca aauaauggac cucgggcaca ugugcgacgc uacaaugucc uaugagugcc      300

cuaugcuaga cgaaggcgug gaaccugacg acguagauug cuggugcaac acaacuucaa      360

ccuggguagu auacggcaca ugccaccaca agaaaggaga ggcacgcagg agccggcgcg      420

ccgucacgcu cccaucacac uccacgcgaa aacuucaaac ccggagccaa acuuggcuag      480

aaagcagaga auacaccaaa caccucauaa gaguagaaaa cuggaucuuc cgaaaccccg      540

gauucgcgcu cgcagccgca gccaucgccu ggcuacucgg cucgagcaca agccaaaaag      600

ugaucuaccu aguaaugaua cuccuaaucg cccccgccua cucaauccga ugcaucggag      660

uaucaaaccg agacuucgua gaaggaaugu caggaggaac uugggucgau auaguacuag      720

aacacggagg cugcgucacg gucauggccc aagacaaacc aacuguugac aucgaauuag      780

uaaccacgac cgucagcaau auggcagagg ugcgauccua cugcuacgaa gccuccaucu      840

cggacauggc aagcgacuca cgaugcccaa cccagggaga ggcguaccuc gacaaacaaa      900

gcgacacaca guacguaugc aaaaggacgc uagucgaccg cggauggggg aacggaugcg      960

ggcucuuugg aaaaggcucg cucguaacau gcgccaaauu cgcgugcagu aaaaagauga     1020

caggcaagag cauacaacca gaaaaccuag aauaccggau caugcucucc guccacggcu     1080

cccaacacag cggaaugaua gucaacgaca ccggacacga aaccgacgag aacagagcaa     1140

aagucgaaau aacacccaac agcccccgcg ccgaagcaac ccuaggaggg uucgggagcc     1200

ucggccugga cugcgaacca cgcaccggac uagacuucag cgaccuauac uaccuaacaa     1260

ugaacaauaa acacuggcua guccacaaag agugguuuca cgacauaccc cugccauggc     1320

acgcaggagc ugacaccgga accccacacu ggaacaacaa agaagcccug gucgaguuca     1380

aggacgccca cgccaaaaga caaacaguag ucguacucgg cucacaagaa ggcgcgguac     1440

acacagcacu cgccggcgcg cucgaagccg agauggacgg cgccaagggc agauuaagca     1500

gcggccaccu caagugcagg cuaaaaaugg acaaacuacg acugaagggc guaucauacu     1560

cacucugcac agcugcauuc acauucacca agauaccagc ggaaacacua cacgggacag     1620

uaaccguaga aguacaauac gccggcaccg acggccccug uaaggucccg gcccaaaugg     1680

cagucgacau gcagacccua accccugucg gaagacugau caccgccaac ccaguaauca     1740

cagaaucaac agagaacuca aaaaugaugc uggaacugga cccgcccuuc ggagauagcu     1800

acaucgugau cggcgucggu gaaaagaaaa ucacgcacca cuggcacaga agcgggagca     1860

ccauagguaa agccuucgag gcgacaguua ggggcgccaa acgaauggca guccucggcg     1920

acacagcaug ggacuucggc agcgugggcg gagcacucaa cucacuaggg aaaggaaucc     1980

accaaauauu cggcgcagca uuuaaaagcc uauucggagg aaugagcugg uucucacaga     2040

uccuaauugg cacacuacua auguggcuag ggcucaacac caaaaacgga agcaucagcc     2100

uuaugugccu cgcacucggu ggaguccuaa uuuuucugag caccgccguc agugcagacg     2160

ugggaugcug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2220

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2280

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2340

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2460

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2500


<210>  7203
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7203
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

cacgcaaaga aaagaagcgc cgcggagccg acacauccgu aggaaucgua ggacuacucc      120

ucacaacggc cauggcagcc gaaguaacca gacgcggcuc cgccuacuac auguaccucg      180

accgaaacga cgcaggagag gcuaucucau uccccacaac ccucggaaug aacaaaugcu      240

auauacagau aauggaccug ggccacaugu gcgacgcaac aaugagcuac gaaugcccaa      300

uguuagauga aggcguagaa cccgacgacg ucgacugcug guguaacacc acaucaaccu      360

ggguaguaua cggcacaugc caccacaaga aaggcgaagc gcgcagaagc cgucgagcag      420

ucacacuacc aucacacuca acacgaaaac uucagacccg aucacaaacc uggcuagaau      480

cacgagaaua uacgaaacac cucauccgag uagaaaacug gaucuucaga aaccccggcu      540

ucgcccuugc agcggcggcc aucgccuggc uccuagggag uucaaccagc cagaagguaa      600

ucuaccucgu caugauccuc cucaucgcac cagccuacag cauccgaugc aucggcguca      660

gcaacagaga cuucguagaa ggaaugagcg gaggcacaug gguagacaua guacucgaac      720

acggcgggug cguaaccgug auggcacaag auaaacccac agucgacauc gaacuuguga      780

ccaccaccgu aagcaacaug gcagaaguaa gguccuauug cuacgaagca ucaauuucug      840

acauggcauc cgacagccga ugcccaacgc agggagaagc cuaccuagac aaacaaagcg      900

acacccaaua cguaugcaaa cgaacgcugg uagaccgcgg cuggggcaac gguugcggac      960

uauucgggaa aggaagccua gucacaugcg ccaaauucgc cugcagcaaa aagaugacag     1020

gcaaaucaau ccaacccgaa aacuuggaau acagaaucau gcuauccguc cacggaagcc     1080

aacacuccgg caugauaguc aacgacacgg gacacgaaac agacgaaaac cgugcaaaag     1140

uagaaaucac cccgaauagc ccaagagcgg aagccacacu agggggcuuu ggcagccucg     1200

gccuagauug cgaaccacga acagggcuag auuucucgga ccuauacuau uuaaccauga     1260

acaacaaaca cuggcuaguu cacaaagaau gguuccacga cauaccgcuc ccguggcacg     1320

ccggcgcaga uacgggcacc ccacacugga acaacaagga agcgcuggua gaguucaaag     1380

augcucacgc caaacgccaa accgucguag uacuaggcuc acaggaaggu gcaguacaca     1440

ccgcacuagc cggagcacuc gaagccgaaa uggacggagc aaaaggacga cucagcagcg     1500

gacaucuaaa augucgacuc aaaauggaca aacucaggcu caagggaguc agcuacagcc     1560

uaugcacggc cgcauucaca uucaccaaaa uaccggcaga aacacuccac gguaccguaa     1620

caguagaggu ccaauacgcc ggcaccgacg gccccugcaa aguaccagca caaauggcag     1680

uagacaugca aacccucacc cccguaggac gacuaauaac ggccaaccca guaauaacug     1740

aaagcaccga aaacaguaag augaugcuag aacuggaccc accauucggu gacucauaca     1800

ucgucaucgg cguaggcgaa aagaaaauaa cccaccacug gcaccgaagc gggucaacca     1860

uaggaaaagc cuucgaagcg acagugcgcg gagccaaacg cauggccgua cugggcgaca     1920

cagcauggga cuucggauca guaggcggcg cccucaacag ccucggcaaa gggauccacc     1980

aaaucuucgg agccgcguuu aaaucgcuau ucggcggcau guccugguuc ucccaaaucc     2040

ucaucggcac ccuacucaug uggcuuggcc uaaacaccaa aaauggcuca aucagccuca     2100

ugugccuagc acucggaggc guccugaucu uccugucaac agcggucucg gcagacgucg     2160

gcuguugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  7204
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7204
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcac       60

gcaaggaaaa gaagcgccgc ggagcagaca ccucagucgg aauagucggc cugcuacuca      120

ccaccgccau ggcggcggaa guaacccgcc gggggucagc cuauuacaug uaccucgacc      180

gcaacgacgc aggcgaagcc aucucauucc ccaccacgcu aggcaugaac aaaugcuaca      240

uccaaauaau ggaccucggc cacaugugcg acgccaccau gagcuacgaa ugcccaaugc      300

uagacgaagg cguagaaccc gacgacgucg auugcuggug caacacaaca ucaacauggg      360

uugucuacgg aaccugccac cauaagaaag gcgaagcacg acgcagccgc agagcaguua      420

cacuacccuc ccacucgaca agaaaauuac aaacacgauc acaaaccugg cucgaaucac      480

gagaauacac caaacaccua auccgcgucg aaaacuggau auuccgaaau cccggauucg      540

cucuagcagc agccgcgauc gccuggcugc ucggcuccuc cacaucccaa aaagucauuu      600

accucgucau gauccuauua aucgcaccgg ccuacucaau acgcugcaua ggaguaucca      660

acagagacuu cgucgaaggg augucaggcg gcaccugggu agacaucgua cuagaacacg      720

gcggcugugu caccguaaug gcccaagaca aaccaaccgu cgacaucgaa cucgucacga      780

ccaccguaag caacauggcc gaaguacgcu ccuauugcua cgaagcaagc auaucagaua      840

uggcaagcga cucccgcugc ccaacacagg gcgaagcaua ccuggacaaa caaagugaca      900

cccaauacgu cuguaagcgg acccucgucg accgcggaug gggcaacggc ugcggccucu      960

ucggaaaagg aucccuagua acaugcgcca aauucgcaug cuccaagaaa augaccggca     1020

aguccauaca accagaaaac cuagaauacc ggaucaugcu aaguguacac ggcagccaac     1080

auuccggaau gauagucaac gacacagggc acgaaaccga cgaaaaccga gcaaaaguag     1140

aaauaacccc aaacuccccc agagcagaag ccacacuagg cggcuuuggc ucucuaggcc     1200

ucgacugcga accaagaaca ggucuagacu ucucagaccu uuacuaccua acaaugaaca     1260

acaaacacug gcucguacac aaagaauggu uccacgacau cccauugcca uggcacgcag     1320

gagcagacac aggaacaccc cacuggaaca acaaagaagc gcuagucgag uucaaagacg     1380

cacacgccaa gagacaaacc guaguagucc ucggcucaca agaaggugcc guccacaccg     1440

cacuagcagg cgcacuagaa gccgaaaugg acggagcaaa aggacgacua uccucaggac     1500

accuaaaaug ccgucucaaa auggauaaac uaagacugaa aggcguauca uacucacucu     1560

gcacagcagc cuucacguuc accaaaaucc ccgccgaaac acuacacggc acagucacag     1620

uagaaguaca auacgccggc accgacgguc ccugcaaggu accagcucaa auggcggucg     1680

acaugcaaac ccuaaccccc guagguagac ucaucacugc aaaccccgug aucaccgaau     1740

caaccgaaaa cucaaaaaug augcuggaac uagacccacc guucggcgac agcuacauag     1800

ucaucggagu aggggagaaa aagauaaccc accacuggca cagaagugga ucgaccaucg     1860

gcaaagccuu cgaagcaaca guucgaggcg ccaaaagaau ggcaguccuc ggagacaccg     1920

cgugggacuu cggcuccgua ggcggagccc ucaacucccu cggcaaggga auacaucaga     1980

ucuucggcgc cgccuucaag agccuuuucg gaggaaugag cugguucuca caaauccuaa     2040

uaggcacacu acucaugugg cucggccuca acaccaaaaa cggguccauc ucccuuaugu     2100

gccuggcacu gggcggagua cucauuuucc ucagcacugc cgucagcgcc gacgucggcu     2160

guugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  7205
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7205
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

aagaaaaaaa acgccgagga gcagacacaa gcgucggcau cguaggauua cuacugacaa      120

ccgcgauggc agccgaaguc acaagacgag gcucagcaua cuacauguac cucgaccgca      180

acgacgcagg ugaagcaaua agcuucccga cgacucuagg aaugaacaaa ugcuauauuc      240

aaauaaugga ccuaggacac augugcgacg caaccaugag cuacgaaugc cccaugcuag      300

acgagggugu cgaacccgac gacguagacu gcuggugcaa caccacaagc accugggucg      360

ucuacggcac augccaccac aaaaaaggag aggcacgcag aagcagaaga gcagucacgc      420

uccccuccca cagcacaaga aaacuacaaa ccagauccca aaccuggcug gagucgagag      480

aauacacaaa acaccucaua aggguagaaa acuggaucuu cagaaacccg ggauucgccc      540

uagcggcagc cgccaucgca uggcuccugg gcucaaguac uagccagaaa gugauauacc      600

uaguaaugau acugcuaaua gcacccgccu acucaauccg cugcaucggc gucucaaacc      660

gagacuucgu cgaagguaug agcggcggua cauggguaga caucguacua gaacauggcg      720

gaugcgucac cgucauggca caggacaaac ccacagugga caucgaacug gucaccacaa      780

ccgucucuaa cauggcagag guacgcuccu acugcuacga agccucaauc ucagacaugg      840

cgagcgacuc ccgcuguccc acccagggcg aagccuaccu ggacaaacaa uccgauacac      900

aauacgugug caaacgcaca cucguugacc gagggugggg gaacgguugc ggccucuucg      960

gcaaaggcag ccuggugacc ugcgcaaaau ucgcgugcuc gaaaaaaaug acaggcaaga     1020

gcauacaacc agaaaaccua gaguaccgga ucaugcuauc aguccacgga ucgcaacacu     1080

caggaaugau agucaacgau acaggacaug aaaccgacga aaaccgagcg aaaguagaaa     1140

ucacacccaa cagcccgcgc gccgaagcaa cacuaggggg cuucggaagc cucggacuag     1200

acugcgaacc acgcaccggc cuagacuuca gcgaccuuua cuaccuaacc augaacaaca     1260

aacacuggcu aguccacaaa gaaugguucc augacauccc gcuaccgugg cacgcuggcg     1320

cagacacggg gaccccacau uggaacaaua aagaagcacu agucgaauuc aaagacgcgc     1380

acgcaaaacg gcaaacaguc gucguacuag gcagccaaga gggagcuguc cauaccgcgc     1440

uagccggagc ccuagaagca gaaauggacg gagcaaaagg ccgccuuagc uccggccacc     1500

ucaaaugucg gcuuaagaug gacaaacuca gacucaaggg cgucagcuac agccucugca     1560

ccgcagccuu cacauucacc aaaaucccgg ccgaaacccu acacggaaca guaacaguag     1620

aggugcaaua cgcgggaaca gacgguccau gcaaaguccc agcccaaaug gcuguagaca     1680

ugcaaacccu uaccccggua gggagacuaa uaacagccaa ccccguaauu acugaaucaa     1740

cagaaaacag caaaaugaug cucgaacucg acccacccuu cggcgauagc uacauaguaa     1800

ucggggucgg agaaaaaaaa auaacacacc acuggcacag aucaggaucc acaauaggaa     1860

aagcauucga agccaccguu aggggagcaa aacgaauggc aguacucggc gacaccgccu     1920

gggacuucgg cagcguaggc ggcgcccuaa acagccucgg aaaaggaauc caccaaauau     1980

ucggugcagc auucaaaagc cucuucggcg gcaugagcug guucagccaa auacucaucg     2040

gcacgcuccu aauguggcua ggacuaaaua caaaaaacgg caguaucagc cuaaugugcc     2100

ucgcgcuagg gggcguccua aucuuccucu caacagcagu cagcgccgac gucggaugcu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  7206
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7206
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

aaaaaaaacg acgcggagca gacacaucag ucggaaucgu aggccuccuc cugacaacag      120

caauggcugc cgaaguaacg aggcggggaa gcgcguacua cauguaccuc gacagaaacg      180

acgcaggcga agccaucucc uucccaacca cauuaggaau gaauaaaugc uacauccaaa      240

uaauggaccu cggacacaug ugcgacgcca caaugucaua cgaaugcccg augcucgaug      300

aaggcgucga acccgaugac guagacugcu ggugcaacac aacgagcaca ugggucgucu      360

acggaacaug ccaccacaaa aaaggcgagg cccgacgauc acgccgagca gugacacucc      420

ccucccacag cacccgcaaa uuacaaacga gaagccaaac auggcucgaa agccgcgaau      480

auaccaaaca ccugaucaga guggaaaacu ggauauucag gaaccccggc uucgcccugg      540

cagcagcagc aauagccugg cuacuuggca guagcacauc ccagaaaguc aucuaccucg      600

ucaugauacu ccucaucgca ccugcauaca gcauccgaug caucggcgug uccaacagag      660

acuucguaga aggcaugagc ggcggaacau gggucgacau aguacucgaa cacggcggcu      720

gcguaaccgu gauggcucaa gacaaaccca cagucgacau cgagcuaguc accacgaccg      780

uaagcaacau ggcagaagua cgcagcuacu gcuaugaagc cucgauaucc gacauggcau      840

cagacucccg gugccccacc cagggagaag cauaccucga caaacaaucc gacacccagu      900

acguuuguaa acgcacacua gucgacagag gcuggggaaa cggcugcggc cuauuuggca      960

aaggcagccu ggucacaugc gcaaaauucg ccugcagcaa aaaaaugacc ggaaaaagca     1020

uacaaccaga aaaccuggaa uaccgcauaa ugcuguccgu ccacggaagc caacacucag     1080

gcaugaucgu aaacgacacc ggccacgaga ccgacgaaaa cagagcaaaa gucgaaauca     1140

caccgaacag cccacgagcc gaagcaaccc ucggaggguu cggcucguua ggacucgacu     1200

gcgaaccaag aaccggacuc gacuucucag accucuacua ccuaacaaug aacaacaaac     1260

acuggcucgu ccacaaagaa ugguuccacg acauaccacu cccauggcac gccggagccg     1320

acaccggcac cccacacugg aacaacaaag aagcccucgu cgaauucaaa gacgcccacg     1380

ccaaacgaca aaccguagua guccucggaa gccaagaagg cgcuguacac acggcccucg     1440

caggggcgcu ggaagccgaa auggauggcg caaaaggaag acuaagcucc ggccaccuaa     1500

aaugccgucu caagauggac aaacugcgcc ucaaaggcgu cucauauagc cuaugcaccg     1560

ccgcuuucac auucacaaaa auacccgccg aaacacucca cggcacagua acagucgaag     1620

uacaauaugc cggcacagac ggcccaugca aagucccagc ccaaauggcc gucgacaugc     1680

aaacccucac accagucggc cgccuaauca cagcaaaccc cguaauaaca gaaagcacag     1740

aaaacucaaa aaugaugcua gaacuggacc cccccuucgg cgauagcuac auagucaucg     1800

gaguuggcga aaaaaaaaua acacaccacu ggcaccgcuc cggcuccacg aucggcaaag     1860

cauucgaagc aacgguacga ggagcaaaaa gaauggcagu acuaggagau acugcauggg     1920

acuucggcuc cguggggggg gcacuaaacu cccuaggcaa aggcauccac caaaucuucg     1980

gcgcagccuu caaaucacuc uucggcggga uguccugguu cagccaaauc cucaucggca     2040

cccuacuaau guggcuuggg cuaaauacga agaacggaag cauaucccuc augugccuag     2100

cacuaggcgg cguccucauc uuccuuagca ccgcaguauc cgcagacgua ggcugcugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  7207
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7207
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaaa       60

agaaaagacg aggagcagac accagcgucg gcaucgucgg ucuacuacuc acaacagcca      120

uggccgcaga agugaccaga cggggaucgg cauacuacau guaccucgac cguaacgacg      180

caggagaagc aaucucauuc ccaaccacac ucggcaugaa caaaugcuac auccaaauaa      240

uggacuuagg acacaugugc gacgccacga uguccuacga augcccgaug cuagaugaag      300

gaguggaacc cgacgacguc gacugcuggu gcaacaccac gagcacgugg guaguauacg      360

gaacauguca ccacaaaaag ggugaagcca gacgcucccg acgggcgguc acccuucccu      420

cacacagcac aagaaaacug caaacucgca gccaaacaug gcuggaauca cgcgaauaca      480

caaaacaccu uauacggguc gaaaacugga ucuuccgaaa cccaggauuc gcacuagcag      540

cggccgccau agccuggcua cuaggcagca guacuucaca aaaaguaaua uaccuaguca      600

ugauacuccu gauagccccc gcuuacucca uccgaugcau cggaguaagc aauagagauu      660

ucguugaagg aaugucagga ggaacauggg uagacauagu ccucgagcac ggcggcugcg      720

uaaccgucau ggcacaggac aaaccaaccg uggacauaga acuggucaca acaacuguca      780

gcaacauggc cgagguacgc aguuacugcu acgaggcauc caucuccgac auggcaucgg      840

acagccgaug cccgacccaa ggagaggcau accucgacaa acaaagcgac acgcaauaug      900

uauguaaacg aaccuuagua gaccgcggau ggggcaacgg uugcggacuc uucggaaaag      960

gcucccuggu cacaugcgca aaguucgccu gcuccaagaa aaugacggga aaaagcaucc     1020

aaccggaaaa ccuagaguac cgaaucaugc ugucugucca cggaucccag cauuccggca     1080

ugaucgucaa cgacacugga cacgaaacag acgaaaaccg agccaaagug gagauaaccc     1140

caaacagccc aagagcagag gcaacccuag gaggauucgg aucccuagga cuggacugcg     1200

aaccgcgcac uggacucgac uucucagacc uauacuaccu uacaaugaac aacaaacacu     1260

ggcucgucca caaagagugg uuccacgaua ucccccuacc cuggcacgcc ggcgcagaca     1320

caggaacacc acacuggaac aacaaagaag cccuagucga guucaaagac gcgcacgcca     1380

aacggcaaac aguagucguc cucggaagcc aagaaggggc uguacauacc gcacucgccg     1440

gcgcccuaga agccgaaaug gacggcgcga aaggacgacu aagcucagga caccucaaau     1500

gccggcucaa aauggacaaa cucagacuca aaggaguuuc cuacucccua ugcacagcag     1560

ccuuuaccuu caccaaaaua cccgcggaaa cccuccacgg aaccgucaca gucgaagucc     1620

aauaugcggg aaccgacggg cccugcaaag ugcccgccca aauggccguc gauaugcaaa     1680

cccucacccc cguaggccga cuaaucacag ccaaccccgu aauaaccgag ucaacagaaa     1740

acuccaaaau gaugcuagaa cuagaucccc cauucggaga cagcuacaua guaaucggcg     1800

uaggcgaaaa gaaaaucacc caccacuggc accgauccgg aagcacaauc ggaaaagccu     1860

ucgaagcaac cguccguggc gccaaaagaa uggccguacu uggagacaca gcaugggacu     1920

ucgggagcgu cggaggagca cugaacagcc ucggaaaagg aauccaccaa aucuucggcg     1980

cagcguucaa gagccuauuc ggcgggauga gcugguucuc acaaauacua aucggaacac     2040

ugcucaugug gcuaggccua aacaccaaaa acggaucaau cagccucaug ugucucgcgc     2100

ugggaggagu acugaucuuc cuaucaacag ccguaagugc cgacgucggc ugcugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  7208
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7208
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

aaagacgggg ugccgacacc ucgguaggaa uaguaggacu ccuacucacc accgccaugg      120

cagcagaagu aacacgccga ggcagcgccu acuauaugua ccucgacaga aacgacgccg      180

gcgaagccau cuccuucccc accacacucg gcaugaacaa auguuacauc caaauaaugg      240

aucucggcca caugugcgac gcaaccaugu cauacgagug cccaaugcuc gacgaaggag      300

ucgaacccga ugacguagac ugcuggugca auaccaccuc cacauggguc guauacggaa      360

cuugccacca uaaaaaagga gaagcaagac gaucacggag agccgucacc uuacccagcc      420

auuccacccg gaaacuacaa accagaagcc aaacguggcu cgagagccgg gaauauacaa      480

aacaccucau aagagucgaa aauuggauau ucagaaaccc cggauucgcc cucgccgccg      540

cagccauagc cuggcuacuc ggauccagca caagccagaa agucauauac cucgucauga      600

uacugcuaau ugccccagcu uauuccaucc ggugcauagg cgucaguaac cgggacuucg      660

uagaaggaau gucaggaggg accuggguag acaucgugcu agaacacgga ggcugcguaa      720

ccgucauggc acaagacaaa ccaacagucg acauagaacu gguaaccacu acagucagca      780

acauggccga aguacgauca uacugcuaug aagcaucaau cagcgacaug gcaagcgaca      840

gucgaugccc cacgcagggc gaagccuacc uagacaaaca aucagacaca caauacgugu      900

gcaaacggac acuugucgac agaggguggg gaaacggcug cggccuauuc gggaaaggca      960

gccuaguaac cugcgcgaaa uucgcgugcu caaaaaaaau gaccggcaaa agcauccagc     1020

cagaaaaccu agaauacagg aucaugcuca gcguccacgg cagccagcac agcgguauga     1080

uagucaacga cacuggccac gaaacagacg agaaccgcgc aaaaguggaa aucacaccaa     1140

acucaccacg cgccgaagca acccucggug gguucggcuc ccuuggacua gacugugaac     1200

caagaaccgg ccucgacuuc agcgaccuau acuaccucac aaugaacaac aaacacuggc     1260

ucguccacaa agaaugguuc cacgacauac cccuaccaug gcacgcaggc gcagacacag     1320

ggacuccaca cuggaacaau aaggaagcgc uagucgaauu caaagacgcc cacgcaaaaa     1380

gacaaaccgu ggucguccug ggaucccaag aaggagcagu gcacaccgcc cucgcaggcg     1440

cucucgaagc cgagauggau ggagcaaaag gaagacuaag cagcgggcac cucaaaugcc     1500

gacuaaaaau ggacaaacua agacuaaaag gcgucagcua cagcuuaugu acggccgccu     1560

ucaccuucac gaaaaucccu gccgaaaccc uacacggcac cguaaccguc gaaguccaau     1620

acgccggaac agacgggccg uguaaagucc ccgcccagau ggcaguagau augcaaacac     1680

ucacccccgu aggacgccua aucacggcaa accccgucau aaccgaaagc acagaaaacu     1740

caaaaaugau gcucgaacua gauccccccu ucggagacag cuacauagua auaggaguag     1800

gagaaaaaaa aaucacccac cacuggcaca gaucaggaag uaccaucggc aaggccuucg     1860

aagccaccgu cagaggagcc aaacgaaugg cgguccucgg agacaccgcc ugggacuucg     1920

gaagcgucgg cggcgcacuc aacucacuag gcaaaggcau acaccaaauc uucggagccg     1980

ccuucaaaag ccucuucggc ggaaugagcu gguucuccca aauacuaauc ggcacccuac     2040

ucauguggcu cggucucaac accaaaaacg gcuccauauc ccucaugugc cuagcacucg     2100

gaggcguccu caucuuccug agcaccgccg ucagugccga cguaggcugu ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  7209
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7209
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

ggagaggcgc agauacgagu guagguauag uagggcucuu gcucacgacc gcaauggcag      120

ccgaagucac aagaaggggc ucggcauacu acauguaccu ggacaggaac gacgccggcg      180

aagcaauauc guucccaaca acgcuaggga ugaauaaaug cuacauacag aucauggauc      240

uagggcacau gugugacgcc acaaugucau acgaaugccc gaugcuggau gaaggcguag      300

agcccgacga cguggacugc uggugcaaca cgacaagcac auggguaguc uacggcacau      360

gccaucacaa gaaaggcgaa gcgagaagaa guaggagagc aguaacacug ccaagccacu      420

caacccgcaa gcuacaaacc cgaagucaga ccuggcuaga aucaagagag uacacaaaac      480

accugauaag aguagagaac uggauauucc gaaacccagg auucgccuug gcagccgcgg      540

caauagccug gcuauuaggc ucguccacca gccaaaaggu caucuaucuc gugaugauac      600

uacuaaucgc ccccgcauac ucaaucaggu gcauaggagu cagcaaccgc gacuuuguag      660

aggggauguc gggcgggaca uggguagaca ucguacuaga acacgggggc ugugucacag      720

ugauggccca agacaaaccc acgguagaua ucgaacucgu gaccacgaca guuagcaaca      780

uggcagaagu uagaucguac ugcuacgaag ccagcauaag cgacauggcc ucagacucgc      840

gaugcccaac gcaaggggag gcauaccugg acaaacaauc agacacccaa uacgucugca      900

aaagaacccu agucgaccga ggcuggggca acgggugcgg gcuauucggc aaaggcagcc      960

ugguaacaug ugcuaaauuc gcaugcagca aaaagaugac aggcaaaagu auacagccag     1020

agaaccugga guacaggauc augcuaagcg uccacggcag ccaacacagc ggcaugaucg     1080

ucaacgacac aggccacgaa acugacgaaa accgagccaa aguagagaua acacccaaca     1140

gcccgcgcgc agaggcaaca cugggcgggu ucgggagccu gggacuagac ugcgaaccga     1200

gaacgggccu ggacuucagc gaccucuacu acuuaaccau gaacaacaaa cacuggcuag     1260

uccauaaaga gugguuccac gacauaccac uaccauggca cgcaggcgca gacaccggga     1320

caccacacug gaacaacaaa gaagcccugg ucgaguucaa ggacgcacac gccaaaagac     1380

agacagucgu gguccugggc ucgcaagaag gagccgucca caccgcccuc gcgggagcac     1440

uagaagccga gauggacggg gccaaagggc gacugagcuc gggacaucuc aaaugccgcc     1500

uaaagaugga uaaacuccgc cucaaaggcg uaucauacag cuuaugcacc gcggcauuca     1560

cauucacaaa gauccccgcc gagaccuugc acgggaccgu gacaguagaa gugcaauacg     1620

ccggaacgga uggaccaugc aaagucccag cacaaauggc agucgacaug caaacccuaa     1680

ccccaguagg acgccuaaua acggcaaacc ccgucaucac agaaucaacc gaaaacucca     1740

aaaugaugcu ggaacuagac ccaccguuug gcgauagcua caucguaauc ggaguaggag     1800

aaaagaaaau cacacaucac uggcaccgaa gcggcagcac aaucggcaaa gcauuugaag     1860

caacaguacg gggcgcaaaa cgaauggcag uacuuggaga caccgcaugg gauuucggga     1920

gcguaggagg ggcccugaac ucgcuaggga aaggaaucca ccaaauauuc ggggccgcgu     1980

ucaagagccu uuucggagga augagcuggu ucagccaaau ucucauaggg acgcuccuga     2040

uguggcucgg gcuaaacacc aaaaacggcu caaucagcuu aaugugccua gcacuaggag     2100

gcguccuaau auuccuaagc acagccguaa gcgcagacgu aggcugcuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  7210
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7210
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcc       60

guggugcaga caccucagua ggaaucguag gccuucuccu aaccacagcu auggccgcag      120

aagucacacg acggggaucg gccuacuaca uguaccucga ccgcaacgac gccggcgaag      180

caaucagcuu cccaaccacc cugggcauga acaagugcua cauacaaaua auggaccucg      240

gccacaugug cgacgcaacg augucauaug aaugccccau gcucgacgaa ggaguagaac      300

ccgacgacgu agacugcugg ugcaacacca ccagcaccug ggucgucuau ggaaccugcc      360

accacaagaa aggcgaggcc cgcagguccc gacgcgccgu aacgcuaccc agccacucaa      420

cucgcaaacu acaaaccaga agccaaaccu ggcuagaauc ccgagaauac acgaaacacc      480

ucauaagagu cgaaaacugg auauuccgca acccaggcuu cgcccuggca gcagcagcaa      540

ucgccuggcu ccuaggcagc agcaccuccc aaaaaguaau auaccuugua augauacugc      600

ucauagcacc cgcauacagc auacgaugca ucggagucag caacagagac uucgucgaag      660

gcaugagcgg aggcacaugg guagacaucg uccuagaaca cggagggugc gucacgguaa      720

uggcacagga caaaccaaca gucgacaucg aacucguuac cacaaccgua agcaacaugg      780

cagaggucag auccuacugc uacgaagcau ccauaagcga cauggcaucc gacagccgau      840

gcccaaccca aggcgaggcu uaccuagaca aacaauccga cacccaguac guaugcaagc      900

gaacucuagu cgauagggga uggggcaacg gaugcggacu cuucggcaaa ggcuccuuag      960

ugacaugugc aaaauucgcc uguagcaaga aaaugaccgg caaaagcauc caaccagaaa     1020

accuggagua ccguauaaug cucagcguac acggaagcca acacagcggc augauaguca     1080

acgacacagg acacgaaaca gacgaaaacc gugccaaagu agaaaucacc ccaaacagcc     1140

cacgagcuga agccacccua ggaggcuucg gaagccuggg acuagacugc gaaccccgca     1200

ccggccucga cuucuccgac uuauacuacc ugacaaugaa caacaaacac uggcuaguac     1260

acaaagagug guuccaugac aucccacuac ccuggcacgc aggagccgac accggcacac     1320

cgcacuggaa uaauaaagaa gcgcucguag aguucaagga cgcccacgcc aagcgacaaa     1380

ccgugguagu ccugggcagc caagaaggag caguccacac cgcccuagca ggagcacuag     1440

aagccgaaau ggacggagca aaagggcgac ucagcucagg acaucucaaa ugccgccuga     1500

aaauggacaa acuccggcuc aagggaguca gcuacagccu gugcaccgca gccuucaccu     1560

ucaccaaaau acccgcagaa acccuccacg gcaccguaac aguagaaguc caauacgcag     1620

gaaccgacgg acccugcaaa guaccagccc aaauggcagu agacaugcaa acacucacac     1680

ccgucgguag auuaauaaca gccaacccag ucauaaccga aucgacugaa aacaguaaaa     1740

ugaugcucga gcuagauccc ccauucgggg acagcuacau aguaaucgga gucggcgaga     1800

aaaagaucac acaucacugg caccgcagcg gcagcaccau cggcaaggcu uucgaagcaa     1860

caguacgcgg ggcaaaacgg auggccgucc uaggcgacac agccugggac uucggcuccg     1920

uaggcggggc acucaacagc cuagggaaag gcauccacca aaucuucgga gccgccuuca     1980

aaucgcuguu cggaggaaug agcugguuua gucaaauacu uauaggcacc cuacucaugu     2040

ggcucggacu aaauaccaaa aacgguucca ucagccucau gugccuagcc cuaggcgggg     2100

uacugaucuu ccucucaacc gccgucuccg ccgacguagg cuguugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  7211
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7211
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gagcugacac gagcgucggc auagucggac uccuccuaac cacagccaug gcagccgaag      120

uaacacgacg uggauccgcc uacuacaugu accuggacag aaacgaugca ggcgaagcua      180

ucagcuuccc caccacccuc ggcaugaaca agugcuacau acagaucaug gaccuaggcc      240

acaugugcga cgcgacgaug agcuacgaau gcccgaugcu cgacgaaggc guggaacccg      300

acgacgucga cugcuggugc aacacaacca gcaccugggu ugucuacggc acgugccacc      360

acaagaaagg agaagcacgc cgaucccgca gagccguaac ccuacccuca cauuccacaa      420

gaaaacuaca aacccgaagc caaacauggc uagaaagccg cgaguacaca aaacaccuaa      480

uaagaguaga aaauuggaua uuccgaaacc ccgguuucgc ccucgcagcc gccgcgaucg      540

ccuggcuacu aggauccucc acuagccaaa aaguaaucua ccucgucaug auacuacuca      600

uagccccagc cuacuccauc cgcugcaucg gagugucaaa cagggacuuc guagaaggca      660

ugagcggagg cacguggguc gacaucguac uagaacacgg cgggugcguc acagugaugg      720

cccaggacaa accaaccgua gacaucgagc uagucacgac cacaguaagc aacauggcug      780

aagucagauc guacugcuac gaagccucca ucuccgacau ggcauccgac ucccgcugcc      840

caacccaagg cgaagcauac cucgacaaac aaucggacac ccaauaugua ugcaaacgca      900

cacucguaga cagaggaugg ggcaacggcu gcggacuauu cggaaaaggg agccuaguca      960

cuugugccaa auucgccugc agcaagaaaa ugaccggaaa aaguauccaa cccgaaaacc     1020

uagaauaccg caucaugcuc uccguccaug gaucccaaca cagcggcaug aucguaaacg     1080

acaccggcca cgaaacagac gaaaaccgcg ccaaagucga aaucaccccc aacuccccaa     1140

gagcagaggc cacccuggga ggauucggau cacuaggccu cgauugcgaa ccgcggaccg     1200

gccuagacuu cucagaccug uauuaccuua ccaugaacaa caagcacugg cucguacaca     1260

aggaaugguu ccacgauaua ccacuacccu ggcacgccgg cgccgacaca ggcacacccc     1320

acuggaacaa caaagaggcc uuagucgagu ucaaagacgc ccacgcaaag cgucaaaccg     1380

uagucguacu uggaucgcaa gaaggcgccg uccacaccgc ccucgcaggg gcccucgaag     1440

cagaaaugga cggcgcgaaa gggcgccuau ccagcggaca ccucaaaugc agacuaaaaa     1500

uggauaaacu ccgccugaaa ggcgucucau acucccucug caccgccgcc uucaccuuca     1560

caaaaauccc cgcagaaacc cuccacggga caguaaccgu cgaaguccag uacgccggaa     1620

cagacggacc cugcaaagua ccugcgcaaa uggcaguaga caugcaaacc cuaacccccg     1680

ugggacgacu aauaacggca aacccaguca uaaccgaauc cacagaaaac ucaaaaauga     1740

ugcucgagcu cgacccaccc uucggcgaca gcuacaucgu caucggaguc ggagagaaaa     1800

agauaacaca ccacuggcac agauccggca gcacgaucgg caaagcauuc gaagccaccg     1860

uacguggagc uaaacgcaug gcaguccucg gagauacagc cugggacuuc ggcuccgucg     1920

gaggcgcccu caacagccua ggcaaaggaa uacaccaaau auucggugca gcauucaagu     1980

cccucuucgg cggcaugagc ugguucucac agauccucau ugggacccua cucauguggc     2040

uaggucuaaa caccaaaaac ggcuccaucu cccucaugug ccuggcacua gguggcguac     2100

ucaucuuccu cucgaccgcg guuucagccg acgucgggug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  7212
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7212
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

cggacacaag cgucgggauc guaggccuuc ugcucacgac cgccauggca gccgaaguca      120

cgcgacgggg aucggccuac uacauguacc uugacagaaa cgacgccggu gaagcaauau      180

ccuucccuac cacccucgga augaacaaau guuauauaca gaucauggac cucggccaca      240

ugugcgacgc aaccauguca uacgaaugcc ccaugcuaga cgaaggagug gagccagacg      300

acguggacug uuggugcaac acaacgagca cauggguagu uuacggaaca ugccaucaca      360

agaaaggaga agcuagaaga uccagacgcg cagucacacu accaucccac agcacgcgca      420

aacuccagac acgcagccaa acauggcugg agucaaggga auacacaaaa caccucauac      480

gagucgagaa cuggaucuuc cgcaaccccg gauucgcacu cgcagccgca gcaauagcau      540

ggcuucucgg gagcuccacc agccaaaaag uaaucuaccu cguaaugaua cuccuaaucg      600

caccagcgua cuccauaaga ugcauaggcg uaagcaaccg agacuucguc gaaggaauga      660

gcgguggaac guggguugac aucguacugg aacacggggg uugugucacg gucauggcac      720

aagacaaacc aacgguggac aucgagcuag uaacaacgac cgucagcaac auggcagaag      780

uccgauccua cugcuacgaa gcaucaauca gcgacauggc cucagacucc cgaugcccca      840

cccaaggaga agccuaccua gacaagcaau ccgacacuca auacgucugc aaaagaacgc      900

uagucgaccg uggaugggga aacggcugcg ggcuauuugg aaaaggaucu cuagucacuu      960

gcgcaaaauu cgcguguagc aagaaaauga caggaaaaag cauacaaccg gaaaauuuag     1020

aauacaggau aaugcucucc guccacggcu cccaacauag cggcaugauc gucaacgaca     1080

caggucacga aacagacgaa aacagggcca aaguagaaau cacccccaac ucaccgcgcg     1140

cagaagcaac acugggcggc uucggcucac uaggccucga cugcgaacca cgcacuggcu     1200

uagacuucag cgaccucuac uaccugacaa ugaacaauaa acacuggcua guccacaaag     1260

aaugguucca cgacauaccg cuaccauggc augcaggagc ggacacaggc acaccacacu     1320

ggaacaacaa agaagcccuc gucgaguuca aagacgccca cgcaaaaaga caaacggucg     1380

uaguccuagg cucccaagag ggggcaguac acacagcacu cgcaggagca cucgaagccg     1440

agauggacgg agcgaaaggc cgacuaucau cgggacaccu caaaugcagg cucaaaaugg     1500

acaaacugcg gcuaaaagga gucuccuacu cacucugcac agcggccuuc acuuucacca     1560

aaaucccagc cgaaacccua cacggcaccg ucaccgugga aguacaauac gccggcaccg     1620

acggcccgug caagguacca gcccaaaugg ccguagacau gcagaccuug accccaguag     1680

gaagacuaau cacagccaac ccggucauaa cagaaagcac cgagaacucc aaaaugaugc     1740

uggaacugga cccacccuuc ggagacagcu auaucgucau aggaguugga gagaaaaaga     1800

ucacccacca cuggcaccga agcggaucaa ccaucggcaa agccuucgaa gccacagucc     1860

gaggcgcaaa aagaauggcc guccuaggcg acacggccug ggacuucggg aguguaggug     1920

gagcacucaa cucacucgga aaaggcaucc accaaauauu cggggccgcc uucaagagcc     1980

uauucggagg caugagcugg uucucgcaaa uacuaaucgg cacacugcuc augugguuag     2040

gccuuaacac uaaaaacggc aguaucagcc uaaugugccu cgcacucgga ggcgugcuca     2100

uauuccugag cacagcagug agcgcggacg ugggaugcug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  7213
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7213
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcag       60

acaccagcgu cggcauagua gggcuacuac ucacaacagc aauggcagcc gagguaacac      120

ggcgcggcuc agccuauuac auguaccucg acagaaacga cgcaggagaa gcaaucagcu      180

uccccaccac gcuaggcaug aacaaaugcu acauacaaau aauggaccua ggccacaugu      240

gcgacgcuac aaugaguuac gaaugcccaa ugcuggacga aggcguagaa ccagacgacg      300

ucgacugcug gugcaacacc acuagcaccu gggucgucua cggaaccugc caccauaaga      360

aaggggaagc gcgccgcagc cggcgagcgg uaacacuccc cagccacagc acgcgcaaac      420

uccagacccg cagccagaca uggcuagaau ccagagaaua caccaaacac cuaauaagag      480

uagagaacug gaucuuccgc aacccaggau ucgcccucgc cgccgcagcc auagccuggu      540

uauuaggcuc cuccacaagc cagaaaguaa ucuaccucgu aaugauacuc cuaaucgcac      600

ccgcauauuc cauaagaugc auaggcguaa gcaaccgcga cuucguugaa gggaugucgg      660

gcggaaccug ggucgacauc gugcucgaac acggaggaug cgucacgguc auggcccaag      720

acaaacccac cgucgacauc gaguuaguca cuacaacagu guccaacaug gccgagguac      780

gcuccuacug cuacgaagca agcauauccg acauggccuc cgacagucgc ugcccaacac      840

agggagaagc auaccucgac aaacagagcg acacacaaua cguaugcaaa cgaacccuag      900

uagaccgagg auggggcaac ggaugcggac uauucggcaa aggcucccua gugaccugcg      960

caaaauucgc augcucaaag aaaaugacgg gaaaaagcau acaaccagaa aaccucgagu     1020

accgaauaau gcuaucgguc cacggcagcc aacacagcgg aaugauagua aacgauaccg     1080

gacacgaaac agacgaaaac agagccaaag uagagauaac ucccaacagc ccacgagcug     1140

aagcgacucu gggaggauuc ggcagccugg gccucgacug ugaaccgcgc accggccuag     1200

acuucucaga ucuuuauuac cucacuauga auaacaaaca uuggcucgua cacaaagaau     1260

gguuccauga cauaccacua ccauggcacg ccggagcaga caccggcacc ccacacugga     1320

acaacaaaga ggcccucgua gaguucaaag acgcccacgc gaaacgacaa acagucgugg     1380

uucuaggcuc acaagaagga gcgguccaca ccgcgcuagc uggcgcacuc gaagcagaaa     1440

uggacggagc aaaaggacgg cucagcuccg gccaccucaa augccgacua aaaauggaca     1500

agcuccggcu gaaaggaguc agcuacuccc ucuguaccgc cgccuucacc uucacaaaaa     1560

ucccagcaga aacacuacac ggcacaguaa caguagaggu ccaauacgcg gggaccgacg     1620

guccaugcaa gguaccagcc caaauggcag uagacaugca gacccuaacg cccguaggac     1680

gccugauaac cgcaaaccca guaauaaccg aaagcaccga aaacagcaaa augaugcugg     1740

aauuagaccc gccauucggc gacagcuaua ucgucauugg cgucggcgaa aagaaaauca     1800

cccaccacug gcaccgcagu ggauccacaa uaggaaaagc auucgaggca accguacgag     1860

gcgcaaaacg aauggccguc cucggagaua cagcguggga cuucggcucc gucggcggag     1920

cacucaacag ccuagggaaa gguauacacc aaauauucgg cgcagcuuuc aaaagccuau     1980

ucggaggaau gagcugguuc agccaaauac uaaucggcac acuccucaug uggcucggac     2040

ucaacaccaa aaacgggagc auaagccuca ugugccuggc gcucggaggc guacucaucu     2100

uccucucaac ggcaguaagc gcagacgugg gaugcugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  7214
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7214
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

ccuccgucgg cauaguggga cuccuccuua cuaccgccau ggcagcggaa guaacccgac      120

gcggcucagc auacuacaug uaccuggacc gcaacgacgc aggcgaggcc aucagcuuuc      180

ccaccacccu gggaaugaac aaguguuaca uacaaaucau ggaccucggc cacaugugcg      240

acgcaaccau gagcuacgaa ugcccaaugc ucgacgaagg aguagaaccc gacgacguag      300

acugcuggug caacacaacc ucgacauggg ucguauacgg aaccugccac cacaagaaag      360

gcgaggcccg ccguucacgc cgagccguca cacuaccauc ccacuccaca agaaaauuac      420

aaacgcgcuc acaaacaugg cucgagucca gagaauacac caaacaccua auccgaguag      480

aaaacuggau cuuccgaaac cccggcuucg cccucgccgc agccgcaaua gcguggcucc      540

ucgguuccag caccucccaa aaagucaucu accuaguaau gauacuacua aucgcuccag      600

cuuacuccau aagaugcaua ggcguaucca accgggacuu cgucgaaggc augaguggcg      660

gcacaugggu cgacaucguc cuagagcacg ggggcugcgu aacagucaug gcccaagaca      720

aaccaacggu cgacauagaa cucgucacca ccacagucuc aaacauggca gaaguccgcu      780

ccuacugcua cgaagcgagc aucagugaca uggcaucaga cagccgcugc ccaacccaag      840

gagaagccua ccuagacaaa caauccgaua cucaauacgu cugcaaaaga acauuagugg      900

acagaggcug gggaaacggc ugcggccucu uuggaaaagg cagccuagua acaugcgcca      960

aauucgcuug cucuaagaaa augaccggca aaucaaucca acccgaaaac cuggaguauc     1020

gcaucaugcu aagcguacac ggcagccaac acagcggaau gauaguuaau gacaccggcc     1080

acgaaacaga cgaaaaccgc gcaaaagucg aaaucacccc caacucaccc cgcgcagaag     1140

ccacccuagg cgguuucggc ucacucggcu uagacugcga accacguaca ggacuagacu     1200

ucucagaccu guacuaccuc accaugaaua acaagcacug gcucguccac aaagaauggu     1260

uccacgacau accccugcca uggcacgcgg gagcggacac cggcacaccc cacuggaaca     1320

acaaagaagc ccuaguggag uucaaagacg cccacgcaaa acgccaaacc guagucguuc     1380

ucggaaguca ggaaggggcc guccacacag cccuagccgg agcccucgaa gcagaaaugg     1440

acggugcgaa agggcgacuc uccucuggac accucaaaug ccgcuuaaaa auggacaaac     1500

uacggcucaa aggagucucc uacagccucu gcacggccgc cuucaccuuc acaaaaaucc     1560

ccgccgaaac acuccacgga acagucacag uagaagucca auacgccgga accgacggcc     1620

ccugcaaagu cccagcacaa auggcagucg acaugcaaac acucacaccc guaggcagac     1680

ucaucaccgc caacccaguc auaacagaau caacagaaaa uuccaagaug augcuggaac     1740

ucgauccccc auucggcgac ucauauaucg ucauaggagu gggagaaaag aaaaucacac     1800

accacuggca ucgcucagga agcaccaucg gcaaagcauu cgaagcuacc guccgaggag     1860

cgaaacggau ggccguacua ggcgacacgg ccugggacuu cggcuccgua ggcggagccc     1920

ucaauucccu agggaaaggc auccaccaaa ucuucggcgc cgccuucaaa ucccuauucg     1980

gcggcauguc cugguucagc caaauacuaa ucggaacacu acucaugugg cuaggacuca     2040

acaccaaaaa cggcuccauc agccucaugu gccuagcucu gggcggaguc cucaucuucc     2100

ucagcaccgc uguauccgcc gacgucggcu gcugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  7215
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7215
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucgguau cguaggccua cuacuaacga ccgcaauggc ugccgaagua acaagacgcg      120

gcagcgcgua cuacauguac cucgaccgaa acgacgcagg cgaagcaauc agcuucccga      180

ccacacuagg caugaacaaa ugcuacauac aaauaaugga ccucggucau augugcgacg      240

caacaaugag uuacgaaugc ccaaugcucg acgaaggagu agagcccgac gacgucgacu      300

gcuggugcaa caccaccagc acaugggucg ucuacggaac gugccaccac aagaaaggcg      360

aagcccgcag aucccgccgc gcagucaccc uccccagcca cucaacgaga aaacuccaga      420

cccggucaca aacguggcuc gaaucccgag aguacacaaa acaccucaua cgcguggaaa      480

auuggaucuu ccgcaacccc ggcuucgcac ucgccgcagc cgccauagcc uggcuacucg      540

gauccucaac aucccaaaaa gucauauacu uaguaaugau ccuacucaua gcaccagcuu      600

acagcauccg cugcauaggc guauccaaca gagacuucgu cgaaggaaug agcggcggaa      660

ccuggguaga cauaguccug gaacacgggg gaugcguaac cgugauggca caggacaaac      720

ccacagucga cauagaacuc gugacaacaa cgguaaguaa cauggcggaa guucgcagcu      780

acugcuacga agccucaauc uccgacaugg caagcgacuc acggugccca acgcagggcg      840

aagccuaccu agacaaacag ucagauacac aauacgucug caaacgaacc cucguagacc      900

ggggcugggg caacggaugc ggccucuucg gaaaaggauc acucgucaca ugcgcaaagu      960

ucgcaugcag uaagaagaug acgggcaaaa gcauccagcc agagaaccuc gaauaccgca     1020

uuaugcucuc aguccacgga ucccaacacu caggcaugau cgugaacgac acaggacacg     1080

aaacagacga aaaccgagcg aaaguagaaa uaaccccaaa cagccccaga gcagaagcaa     1140

cccucggcgg cuucggcagu cucggacuag acugcgaacc aagaacaggc cucgacuuca     1200

gcgaucugua cuaccuaacg augaacaaca aacacuggcu cguacacaaa gaaugguucc     1260

acgacauacc acucccgugg cacgcaggag cagacacggg cacaccccau uggaacaaca     1320

aagaggcccu agucgaguuc aaggacgccc acgccaaacg gcagaccguc gugguacuag     1380

gcucacaaga aggagcagug cacacagcac uagcaggagc acuugaggca gaaauggacg     1440

gcgccaaagg ucgacuguca agcgggcacc uaaaaugucg ccuaaaaaug gacaaacuac     1500

gccucaaagg cguaagcuau agccucugca ccgcagcguu cacguucacg aaaauaccgg     1560

cagagacccu acacggcaca guaacgguug aaguccaaua cgccggcacc gacgggccau     1620

gcaaaguccc ggcccaaaug gccgucgaca ugcaaacgcu aacaccgguc ggacgccuca     1680

ucacagccaa cccggucauc acagaaagca cagagaacag uaaaaugaug cuagaacuag     1740

acccacccuu cggcgacuca uacaucguaa uaggcguagg agagaagaaa auaacccacc     1800

acuggcacag aagcggcagu accauaggca aagcauucga agcaaccgua agaggcgcca     1860

aacgcauggc aguacuaggc gacaccgccu gggacuucgg cagcguagga ggagcccuaa     1920

acucccucgg caaaggcaua caccaaauau ucggcgcagc cuucaaaagc cucuucggcg     1980

ggaugagcug guucucccag auacuaauag gcacccuacu uauguggcua ggacuaaaca     2040

ccaaaaacgg aagcaucagc cucaugugcc ucgcacucgg cggaguacua aucuuccucu     2100

ccacagcggu cucagcagac gucggcuguu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  7216
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7216
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

caguuggaau cgucggccua cuucugacaa ccgccauggc agccgccguc acacgcaggg      120

gcucagcaua uuacauguac cuggaccgaa acgacgccgg ggaggccauc uccuucccca      180

ccacccucgg gaugaacaaa ugcuacaucc aaauaaugga ccugggccac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggagu agaacccgac gaugucgacu      300

gcuggugcaa caccacgagc accugggugg ucuacggaac gugccaccac aagaaaggcg      360

aagcccgacg cucccgcagg gcggugaccc uacccagcca cagcacacgg aaacuacaaa      420

ccagguccca gacauggcuc gaguccagag aauacaccaa acaccuaauu cgcgucgaaa      480

acuggaucuu cagaaaccca ggauucgccc uagccgcagc cgccaucgca uggcuccucg      540

gcucaagcac aagccaaaaa gucaucuacc uagucaugau acugcuaauc gcccccgccu      600

acucaauccg cugcaucgga guaucaaauc gcgauuucgu ugagggaaug agcggaggca      660

ccugggucga caucguccuc gaacacggcg gcugcguaac agucauggcu caagacaaac      720

caacaguaga caucgagcua guaacaacca ccguaagcaa cauggcagaa guacggucau      780

acugcuacga agcaucuaua ucagacaugg ccucagacuc acgaugccca acucaagggg      840

aagcauaucu cgacaaacaa agcgacaccc aauauguaug caaacgaaca cucguugaca      900

gaggcugggg aaacggcugu ggccucuucg gcaaaggcuc ccuugucaca ugcgcaaaau      960

ucgccugcuc gaagaaaaug accggcaaaa gcauacaacc agaaaaccug gaauacagaa     1020

uaaugcugag cguacacggc ucgcaacaua gcggcaugau aguaaaugac accggacacg     1080

aaaccgacga aaaccgagcc aaaguagaaa ucacccccaa uucuccccgc gccgaagcca     1140

cucugggagg cuucggaucc cuaggccucg acugcgaacc aagaaccgga cuagacuucu     1200

cagaccuaua cuaccuaaca augaacaaca aacacuggcu uguccacaaa gaaugguucc     1260

acgacauccc ccuacccugg cacgcaggcg ccgacacagg aacgccccac uggaacaaca     1320

aagaagcacu cguagaguuc aaagacgccc acgcgaaacg ccaaaccgua gucguccugg     1380

gcucacaaga aggagcaguc cacaccgcgc ucgccggugc acucgaagca gaaauggacg     1440

gcgccaaagg ccgacuaagc uccggccauc uaaaaugccg ucuaaaaaug gacaaacucc     1500

gccuaaaagg agucagcuac ucacugugua cagcagccuu caccuucacc aaaaucccgg     1560

ccgagacgcu acacggcacc gucaccgucg aaguucagua cgcagggacc gacggacccu     1620

gcaaaguccc cgcccaaaug gcgguagaca ugcaaacccu gaccccgguc ggaagacuua     1680

uaaccgcaaa ccccguaauc accgaaucaa ccgagaacag caaaaugaug cuggagcuag     1740

acccacccuu cggcgacucc uacaucguaa ucggcgucgg cgaaaagaaa auaacccacc     1800

acuggcaccg aucgggauca accauaggaa aagcguucga agcaaccguc cgcggagcaa     1860

aacggauggc cgugcuagga gacacagcau gggacuucgg aagcguagga ggcgccuuaa     1920

acucacuagg caaaggcaua caccaaaucu ucggcgccgc cuucaagagc uuauucggag     1980

gcaugagcug guucucccaa auccuaaucg gcacacuccu cauguggcug ggacucaaca     2040

ccaaaaacgg gagcauaucg cucaugugcc ucgcccuggg uggcguccua aucuuccuau     2100

cgacagccgu cuccgccgac gucggcugcu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  7217
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7217
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgccgacac cagcguaggc aucgucggac uacuccucac cacagccaug gcagccgcag      120

uaacacgacg aggaucagcc uacuacaugu accuggacag aaaugacgcc ggcgaagcca      180

ucagcuuucc aacaacucuc gguaugaaca agugcuacau acaaauaaug gaccucggcc      240

acaugugcga cgcaacaaug agcuacgaau gccccaugcu agacgaaggg gucgagcccg      300

acgacguaga cugcuggugc aacaccaccu caaccugggu gguauacgga acaugccacc      360

acaagaaagg cgaagcucga cgcagcagac gagccguaac acucccaagc cacagcacaa      420

gaaaacucca aaccagaagc caaacauggc ucgaauccag agaauacaca aaacaccuaa      480

uuagaguaga aaacuggaua uucagaaacc ccgguuucgc acucgccgcc gccgcaauag      540

cauggcuacu cggaagcucc accucgcaaa aagugaucua ccucgucaug auccuacuaa      600

ucgcaccagc cuacuccauc agaugcaucg gagucuccaa ccgagacuuc gucgaaggaa      660

ugagcggcgg aaccugggua gacaucgugc uagaacacgg cggaugcguc acuguaaugg      720

cacaagacaa accaacaguc gacaucgaac ucguaaccac aacggucucc aacauggccg      780

aaguccgauc cuacuguuac gaggccagca uaucagauau ggcauccgac agccgaugcc      840

ccacccaagg cgaagccuac cuagacaaac aaagcgacac ccaauacgua ugcaaacgca      900

cccucguuga cagaggcugg ggaaacggau gcgggcuauu cggcaagggc ucacucguca      960

caugcgccaa auucgccugc agcaaaaaga ugaccgggaa aagcauacaa cccgaaaacc     1020

uagaauaccg aaucaugcua ucagugcacg ggucacagca uagcggcaug aucgucaacg     1080

acaccggaca cgagacggac gaaaacaggg ccaaagucga gaucaccccc aacucaccgc     1140

gcgcagaagc cacgcucggc ggauucggcu cauuaggacu ggauugcgaa ccacguaccg     1200

gccucgacuu cuccgaccuc uacuaccuca caaugaauaa caaacacugg cugguacaca     1260

aagaaugguu ccacgacaua ccccuacccu ggcacgccgg agccgacaca ggcacaccgc     1320

acuggaacaa caaggaagcc cucgucgagu ucaaagacgc ccacgcuaaa agacaaaccg     1380

ucgucguacu aggaagccaa gaaggcgcag ugcacaccgc gcuggcgggc gcacuugaag     1440

ccgaaaugga uggagcaaaa ggaagacuaa gcuccggaca ccugaaaugu cgccuaaaga     1500

uggacaaacu aaggcuaaaa ggcguaucgu acucacucug cacagcagca uucacuuuca     1560

caaaaauacc cgccgaaacg cuccacggca ccguaacagu cgaaguccaa uacgcgggaa     1620

ccgacggccc augcaaaguc cccgcccaaa uggccgugga caugcagacc cuaaccccgg     1680

ugggccgccu aauaacagca aaccccguaa ucacugaaag caccgaaaau ucaaaaauga     1740

ugcucgaacu cgacccaccc uucggagaca gcuacauagu gauaggagua ggagagaaaa     1800

agauuaccca ccauuggcac agaucaggca gcacaaucgg aaaggcuuuc gaagcaaccg     1860

uacgcggagc aaaacgcaug gcgguacucg gcgacacagc cugggacuuc ggcagcgucg     1920

gcggcgcacu aaacuccuua ggaaagggua uccaccaaau cuuuggcgca gccuucaaaa     1980

gccucuucgg cggcaugagc ugguucucac aaauccucau aggcacacuc cucauguggc     2040

ucggcuuaaa caccaaaaac ggauccauca gccuaaugug ccuggcacuc ggcggcgucc     2100

uaaucuuucu aucaacugcc gucagugcag acguaggaug cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  7218
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7218
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gccucagcuc cagaaaacga cgaucacacg acguccucac gguacaauuc cucauacuag      120

guaugcuacu caugaccgga ggagugacac gcagaggcag cgccuacuac auguaccucg      180

accgcaacga cgcaggagag gccaucagcu uccccacaac ccuaggcaug aauaaaugcu      240

acauccaaau cauggaccua ggccacaugu gcgacgccac aaugucauac gaaugcccca      300

ugcuugacga aggaguagaa cccgacgacg uagacugcug gugcaacaca accagcaccu      360

gggucguaua cggaacgugc caccacaaga aaggugaagc acgaagaagc cgaagggcag      420

ucacccuucc cagccacuca accagaaaac uccaaacacg aucccaaacc uggcuagaga      480

gccgcgagua uacgaaacac cucauccgcg ucgaaaacug gaucuucaga aacccaggcu      540

ucgcgcucgc ugcagcagca auagcauggc uccugggaag cagcaccucg caaaagguua      600

uauaccuagu caugauacua cugauagcac ccgccuacag cauacgcugu aucggaguau      660

ccaacagaga cuucgucgaa ggaauguccg gaggaacaug gguagauaua gugcucgaac      720

acggugggug cguaaccguc auggcccagg acaaacccac cgucgacaua gaacucguca      780

caaccacggu aucaaacaug gccgaaguac gaucauacug cuacgaagca agcauuagcg      840

acauggcguc cgacucaagg ugcccaaccc aaggagaagc auaccuagac aaacaaagcg      900

acacgcaaua cgugugcaag cgaacccuug uagacagagg augggggaac ggaugcggcc      960

ucuucggaaa aggcagccua guaacaugcg caaaauucgc augcagcaag aaaaugaccg     1020

gaaaaucgau acaaccagag aaccucgaau acagaauaau gcucagcguc cacgguucac     1080

agcacagcgg caugauagug aacgacacag ggcacgagac cgacgaaaau cgcgcuaaag     1140

ucgagauaac cccaaacagc ccacgagcag aagccacccu cgggggcuuc ggaucacuug     1200

gacuagacug cgaaccacga accggacuag auuucagcga ccucuacuac cuaacaauga     1260

acaacaaaca cuggcucgua cacaaagaau gguuccacga uaucccguua ccauggcacg     1320

caggagccga cacaggaacu ccccacugga acaacaaaga agcacucgug gaguucaagg     1380

acgcucacgc aaaacgccaa accguggugg uccuaggaag ccaggaagga gccguucaca     1440

cagccuuagc cggagcacua gaagcggaga uggacggggc caagggccgc cugagcagug     1500

gacaccucaa gugccgacug aaaauggaca aacuaagacu aaaaggaguc ucguacagcc     1560

uaugcacagc agccuucacc uucacaaaga uaccggccga aacacuacac ggcacaguga     1620

caguagaagu acaauacgca ggaaccgacg gccccugcaa aguacccgcc caaauggcag     1680

uagacaugca aacccucaca cccguaggca ggcuaaucac ggcgaaccca guuauaacgg     1740

aaagcacgga gaacagcaaa augaugcuag agcuggaccc accauuugga gacucguaca     1800

uaguaaucgg agucggagaa aagaaaauca cacaccacug gcaccguucc ggcagcacga     1860

uaggcaaggc guucgaagca acagucagag gcgccaagag gauggcggua cucggagaca     1920

ccgccuggga cuucggcuca gugggcggcg cgcuaaacuc acucggaaaa ggcauacacc     1980

aaauauucgg agccgcauuc aaaagucuuu ucggaggcau gagcugguuc agccaaaucc     2040

ugauaggcac cuuacuaaug uggcucggcu uaaacaccaa aaacggaagu aucucacuca     2100

ugugcuuagc ccugggcgga guucucauau uccuuagcac cgcagucagc gccgacguug     2160

ggugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  7219
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7219
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccucucuag ccgcaaacgc agaagccacg acgucuuaac uguacaauuc uuaauccucg      120

gcaugcugcu aaugacaggg gcaguaacac gccgaggauc cgcuuacuau auguaccugg      180

accggaacga cgcaggcgaa gccauauccu ucccaacaac ccugggcaug aacaaaugcu      240

acauacaaau aauggaccua ggucacaugu gcgacgcgac caugagcuac gagugcccca      300

ugcucgauga aggagucgaa cccgacgacg ucgacugcug gugcaacacc acgucuaccu      360

ggguagucua cggaaccugc caccacaaaa agggagaagc acggcgaucu aggcgagcag      420

uaacgcuacc aucacauagc acgcggaaac uccagacacg aucccaaacc ugguuagaaa      480

gccgagagua caccaaacac cugauaagag uagaaaacug gaucuucaga aacccaggau      540

ucgcucuagc ggcagcagcc aucgccuggc uccuuggauc aucaacaucu caaaaaguaa      600

ucuaccucgu aaugauccuc cucaucgcac cggcauacag cauacgcugc aucggcgucu      660

caaacagaga cuucguagaa gggaugagcg gcggcacaug gguagacaua guacucgaac      720

acggcggcug cguaaccgua auggcgcagg acaaaccaac cgucgacauc gagcuaguca      780

cgaccaccgu cucaaacaug gcugaagucc gaucauacug cuacgaagca agcaucagcg      840

auauggccuc cgacaguaga ugccccaccc aaggagaagc cuaccuugac aaacaauccg      900

acacccaaua cguaugcaag cgaacccucg ucgaccgcgg cuggggcaac ggcugcggcc      960

uauucggcaa aggcagccuc gucacaugcg ccaaauucgc cugcagcaag aaaaugaccg     1020

gaaaaucaau acaaccagaa aaccucgaau accgaaucau gcucagcguc cacggcagcc     1080

aacacuccgg caugauaguc aacgacacag gccacgaaac agacgagaac cgugccaaag     1140

uagagauaac acccaacagu ccccgugccg aagcaacccu cggcggcuuc ggcucccuag     1200

gccuggacug cgaaccacga accggacuag acuucagcga ccuauacuac cuaacaauga     1260

acaacaagca cuggcuggua cacaaagaau gguuccacga caucccccuc cccuggcacg     1320

cgggcgccga cacgggaacc ccacacugga acaacaaaga agcccucguc gaguucaaag     1380

acgcgcacgc aaaaagacaa acagucgucg uauuaggcuc acaagaaggc gcaguacaca     1440

cagcccuagc uggagcccuc gaagcagaaa uggacggggc aaaaggccga cucagcagcg     1500

gacaccucaa augccgccuu aaaauggaca aacuacgacu aaagggaguc agcuacucac     1560

ucugcaccgc cgcauucacg uucaccaaaa uccccgccga aacccuccac ggaaccguaa     1620

caguagaagu acaauacgca ggcaccgacg gucccugcaa agucccagcc caaauggcag     1680

uagacaugca gacacuaacg ccuguaggga gacuaauuac agcaaacccu gucaucacgg     1740

aaucaaccga gaacagcaaa augaugcuag aacuagaucc cccauucgga gacaguuaca     1800

ucguaauugg agucggagaa aagaagauaa cgcaccacug gcacagaagc ggcucaacaa     1860

uaggaaaagc auucgaagcc acaguccggg gagccaagag aauggccgua cugggggaca     1920

ccgcauggga cuucggaagc gugggcggag cacuaaacag ccuaggcaaa gggauacacc     1980

aaauauucgg cgccgccuuc aaaucacucu ucggaggcau gagcugguuc agccagauuc     2040

uuaucggcac acuacuaaug uggcucggcc ugaacaccaa aaacgguagc auaucacuca     2100

ugugccuugc ccucggaggu guacuaaucu uccugagcac cgccgucagc gcagacguag     2160

gaugcugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  7220
<211>  2497
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7220
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaacgc acgaaaagag aagaaaagaa gaggcgccga cacaagcgug ggaauagucg      120

gccuacugcu aacaacagcc auggccgccg aagucacgcg ucgaggaucc gccuacuaca      180

uguaccucga ccgaaacgac gccggcgaag ccauauccuu cccaacaacc cucggcauga      240

acaaaugcua cauccaaaua auggaccugg gacacaugug cgacgccacc augucauacg      300

aaugcccaau gcuagacgaa ggcgucgaac cagacgaugu cgacugcugg ugcaacacaa      360

cuagcacaug gguaguauac ggcacaugcc accacaagaa aggggaagcc cgccgcagcc      420

ggagggccgu caccuuacca ucccacucaa cgcgcaaacu ccaaacccga agccaaacau      480

ggcucgaauc gcgagaauac accaaacacc ucauccgggu cgagaacugg aucuuucgaa      540

accccggguu cgcccuagcg gcggcggcaa uagcauggcu acucggcuca ucaacgucuc      600

aaaaagucau uuaucuaguc augauacugc uaaucgcccc agcguacuca auacggugca      660

ucggaguauc aaaccgcgau uucguggagg gcaugucagg cggaaccugg guagacauag      720

ugcuagagca cggaggcugu guuacuguca uggcccagga caaacccacc guggacaucg      780

agcuaguaac aacaaccgua uccaacaugg cagaaguccg uucauacugc uaugaagccu      840

ccaucuccga cauggcauca gauagcagau gcccaacaca gggcgaagca uacuuagaca      900

aacagucaga cacgcaauac guuuguaaac gcaccuuagu cgacagaggc ugggggaacg      960

gaugcgggcu auucggcaag ggcucgcuag uaaccugcgc caaauucgca ugcagcaaga     1020

agaugaccgg caaauccaua caaccagaaa accucgaaua cagaauaaug cuaucugucc     1080

acggcagcca acacagcgga augauuguca acgacacagg ccacgaaacg gacgaaaacc     1140

gagccaaagu agaaaucaca cccaacucac cgcgagccga agcaacccuc gggggcuucg     1200

gcucccuagg acucgacugc gaaccccgga ccggccucga cuucagcgac cucuacuacc     1260

uaaccaugaa caacaaacau uggcuagucc acaaagaaug guuccacgac aucccccuuc     1320

cauggcaugc aggagcagac acaggcacac cccacuggaa caacaaagaa gcgcuaguag     1380

aguucaaaga cgcacacgca aaacgacaaa cagucgucgu gcugggaucc caagaaggcg     1440

cuguccauac agcacucgca ggcgcacucg aagccgagau ggauggcgca aaaggacgac     1500

uaagcuccgg acaccucaaa ugcagacuga aaauggacaa acucagacua aaaggagucu     1560

cauacucccu augcaccgcc gcguucaccu ucaccaaaau accagcagaa acacuccacg     1620

gaacggucac cgucgaagua caauaugcag gcacagacgg accgugcaaa gucccggccc     1680

aaauggcagu cgauaugcaa acacuaaccc cagucggccg ccugaucacu gcaaaccccg     1740

uaaucacaga aagcacagaa aacuccaaaa ugaugcucga auuggaccca cccuucgggg     1800

auagcuacau cguaaucggc gucggcgaaa agaaaauaac acaccacugg caccgcagug     1860

ggagcacuau aggcaaagca uucgaagcca ccguucgcgg agccaaacgc auggccguac     1920

ucggcgacac agccugggau uucggcucag uaggcggcgc ccuaaacucc cuagggaaag     1980

gcauccauca aauauucgga gcugcguuca aaagccuauu cggaggaaug agcugguucu     2040

cccaaauacu caucggcaca cuacugaugu ggcucggccu aaacaccaaa aacggaucca     2100

uaagccuaau gugccucgca cucggcggcg ugcucaucuu ccucagcaca gccguaagcg     2160

cagacugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2220

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2280

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2340

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2460

ccccccccca aaggcucuuu ucagagccac cagaauu                              2497


<210>  7221
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7221
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

uaaacgcacg caaggaaaag aaacgaagag gcgcugacac aagcgucggg auaguaggcc      120

uccugcuaac caccgcaaug gcagcagaag ugacucgccg uggcagcgca uacuacaugu      180

accucgaccg uaacgacgca ggggaagcaa uaaguuuccc caccacacuc ggcaugaaca      240

aaugcuacau ccaaaucaug gaccucggcc acaugugcga cgccaccaug agcuacgagu      300

gccccaugcu cgacgaggga gucgaaccag acgacguaga cugcuggugu aacacgaccu      360

caaccugggu gguauacgga accugccacc auaagaaagg agaagcccgc cgaucccgac      420

gcgcaguaac ccuccccuca cacuccaccc gaaaacuaca aacacgcuca caaacauggc      480

uggaaucacg cgaauacacc aaacaccuca ucagaguaga gaauuggauc uuccgcaacc      540

ccggcuucgc cuuagcagcc gccgccauag cauggcuacu cgggagcucu accagccaaa      600

aggucauaua ccuaguaaug auccugcuaa ucgccccagc auacagcaua cgcugcaucg      660

gcguauccaa cagagacuuc gucgaaggua uguccggcgg cacauggguc gacaucgucc      720

ucgaacacgg aggcugcgug accgucaugg cccaagauaa accaaccguc gacauagaac      780

ucgucaccac cacaguauca aacauggccg agguacgcuc auacugcuac gaagcaucca      840

uauccgacau ggcaagcgac ucgaggugcc cuacacaggg cgaagccuac cuagacaaac      900

aaucagacac acaauacguc uguaaacgca cccuagucga cagaggaugg ggaaacgggu      960

gcggccuauu cgggaagggc agccugguaa ccugcgcaaa auucgccugc agcaagaaaa     1020

ugacuggaaa aucgauccaa ccugaaaacc uggaauaccg aaucaugcua agcguccacg     1080

gaagccagca cucgggcaug aucgucaaug acacaggaca cgaaacagac gaaaaucgcg     1140

caaaaguaga aauaaccccc aacucgccca gggccgaagc cacgcucggc ggcuucggau     1200

cgcuaggccu ugacugcgag cccagaaccg gacuagacuu cucagaucug uacuaccuaa     1260

ccaugaacaa caaacacugg cucgugcaca aagaaugguu ucacgacaua ccccucccau     1320

ggcacgccgg cgcagacaca ggcacacccc acuggaacaa caaagaagca cucguagagu     1380

ucaaagacgc ccacgcaaaa cgccaaacag ucgucgugcu aggcucccaa gaaggagccg     1440

uccacacagc ccucgccggc gcccuagaag ccgaaaugga cggagccaaa ggacgacucu     1500

caagcgggca ucucaaaugc aggcuaaaaa uggauaagcu cagacuaaaa ggagucagcu     1560

acucacuaug caccgccgcc uucaccuuca ccaaaauacc cgcggaaaca cuacacggaa     1620

caguaaccgu cgaaguccaa uacgccggca ccgauggccc gugcaaaguc cccgcucaaa     1680

uggccgucga caugcaaaca uuaacgcccg uaggccgccu caucacagca aauccaguca     1740

uaacggaaag cacagaaaac agcaaaauga ugcucgaacu cgacccgccc uucggagaca     1800

guuacaucgu cauuggcgua ggcgaaaaga aaauaacaca ccacuggcac agaagcggau     1860

cgaccauagg caaagcauuc gaagcaaccg uccgaggcgc caaaagaaug gccguacucg     1920

gagauacagc augggacuuc ggaucagucg gcggagcccu aaacucccuc ggcaaaggua     1980

uacaccaaau cuucggagcc gccuucaaaa gccucuucgg aggcaugucg ugguucaguc     2040

aaauacucau aggaacacua cucauguggc uaggccuaaa caccaaaaac ggcucuauca     2100

gccucaugug ccuagcacua ggaggcgucc uaaucuuccu cagcacagcg guauccgccg     2160

acugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  7222
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7222
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauca       60

acgcccggaa agaaaagaaa aggagaggcg cagacacaag cgucggcaua gucggacucc      120

uacucacaac cgccauggcg gccgaaguca cacggagagg aucggccuac uacauguacc      180

uagaccgaaa cgacgccgga gaagccauca gcuuccccac aacacuagga augaacaaau      240

gcuacauaca aauaauggac cuuggccaua ugugcgacgc cacaaugagc uaugaaugcc      300

ccaugcucga cgaaggaguc gagccggacg acgucgauug cuggugcaac accacaucca      360

caugggucgu cuacggcaca ugccaccaca agaaaggaga agccagacgc agcagacgag      420

ccguaacacu acccagccac ucaacacgca aacuccaaac ccgaagccaa accuggcucg      480

agagcagaga auauaccaaa caccuaauca gggucgagaa cuggauauuc aggaacccag      540

gauucgcacu agcugccgca gcaauagcau ggcuacuagg cagcagcacc agccagaaag      600

ugaucuaccu cguaaugaua cuucucauag cacccgccua cucaauacga ugcauaggcg      660

ucagcaaccg cgacuucguc gaaggcaugu cagguggaac auggguagac aucgugcucg      720

aacacggcgg cugcgucacc gucauggccc aagacaaacc caccguagac auagagcuag      780

ugaccacaac aguaagcaau auggccgaag ugagaagcua cuguuacgaa gccagcauca      840

gcgacauggc guccgacucu cgcugcccaa cccaaggaga agcguaccua gacaaacaaa      900

gcgacacaca auacguaugc aaaaggaccc ucgucgaccg aggcuggggc aacggaugcg      960

gacuguucgg caaagggagc cuaguaacau gcgcaaaauu cgcgugcucc aagaaaauga     1020

caggaaaaag cauacaacca gagaaccuag aauaccgcau caugcuaagc guccacggaa     1080

gccaacacuc aggcaugaua guuaacgaca caggccacga gaccgaugag aaccgcgcca     1140

aaguggagau cacaccaaac uccccaagag cagaagcgac ccuaggagga uucggaucgc     1200

ucgggcucga cugcgagcca agaacaggcc uagacuucuc cgaccucuac uaccuaacca     1260

ugaacaauaa acauuggcua gugcacaaag aaugguucca cgacauaccg cucccauggc     1320

acgccggcgc ugacacggga acgccccacu ggaacaauaa agaagcacug gucgaguuca     1380

aggacgcaca cgccaaaaga caaaccgucg uaguacucgg aagccaagaa ggcgccguac     1440

acaccgcacu ggccggagcc cuagaagcag aaauggacgg cgcgaaagga agacucagcu     1500

caggacaccu gaagugccgg cucaaaaugg acaaacuccg acucaaagga guaagcuaca     1560

gccucugcac agccgccuuc accuucacca agaucccggc agaaacccuu cacggcaccg     1620

ucacaguaga aguacaauac gcagguacag acggacccug caaggucccc gcacaaaugg     1680

caguagacau gcaaacccug acaccaguug ggagacugau caccgcaaac ccagugauca     1740

ccgaaucaac cgaaaacagc aaaaugaugc uagaacucga cccaccauuc ggggacagcu     1800

acauaguaau cggcguggga gaaaagaaaa uaacacacca cuggcaccgc ucaggcagua     1860

cuauaggaaa agccuucgag gccacagucc guggggccaa aagaauggcg guacuaggcg     1920

acaccgccug ggacuucggc agcguagggg gcgcacuaaa cucacucgga aaagggauuc     1980

accagauuuu cggcgcagcc uucaaaagcc ucuucggagg aaugagcugg uucagccaaa     2040

uacucauagg aacgcuacug auguggcugg gccucaacac caaaaacggg ucaauaagcc     2100

ucaugugccu agcccuaggc ggaguccuaa uauuccuauc cacagcaguc agcgcagacu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  7223
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7223
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaug       60

cacgaaaaga aaagaaacga cgaggagcag acacuucagu cggcaucgug ggccugcuac      120

ucacaacagc gauggccgcc gaagucacac gccgaggcuc ugcuuacuac auguaccucg      180

aucgaaacga cgccggggaa gcaaucucgu uccccaccac gcugggaaug aacaaauguu      240

acauacaaau cauggaccuc ggccacaugu gcgacgccac cauguccuac gaaugcccaa      300

ugcucgacga aggaguagag ccagacgacg ucgacugcug gugcaacacc accagcacgu      360

ggguagucua cgggacgugc caccauaaga aaggagaagc acgcagaagu agacgagcag      420

ucacccuacc aagccacuca acccgcaaac uacaaaccag aucucaaacg uggcucgaaa      480

gucgcgagua cacuaaacac cucauacgcg ucgaaaacug gaucuuccga aaucccggau      540

ucgcgcuggc agcagcagcc auagccuggu uacugggcag cuccaccagc caaaaaguaa      600

ucuaccuugu gaugauccua cuaaucgcuc cagccuacag cauccgaugc auaggcguca      660

gcaaccgaga cuucgucgag ggcauguccg gcggaaccug gguagacauc guacuagaac      720

acggcgggug cgucacaguc auggcacaag acaaacccac cgucgacaua gagcuaguaa      780

ccacgacagu aagcaacaug gcagaaguca gaagcuacug uuacgaagca ucaauauccg      840

acauggcaag cgacucccga ugccccaccc aaggcgaagc auaccuagac aaacaaagcg      900

acacccagua cgucugcaaa aggacccuag ucgaccgagg cuggggaaac gggugcggac      960

uguucgggaa aggcagccuc guaaccugcg ccaaauucgc cugcucaaag aaaaugacgg     1020

gcaaaagcau acaacccgaa aaccucgaau acaggaucau gcuaucuguc cauggcagcc     1080

aacacuccgg caugauaguc aaugauacug gacacgaaac agacgaaaac cgcgccaaag     1140

uagaaaucac acccaacagc ccccgcgcag aagcaacccu cggaggcuuc ggaucccuag     1200

gccucgacug cgaaccacgc accggccucg acuucuccga ucucuacuac cucaccauga     1260

acaauaaaca cuggcugguc cacaaagaau gguuccacga cauaccccug ccguggcacg     1320

ccggcgccga cacaggaacc ccacacugga acaacaaaga ggcgcuaguc gaguucaaag     1380

acgcccacgc uaaacgacaa acggucgucg uccuaggcuc ccaagaaggg gcgguccaca     1440

cagcccucgc aggagcccug gaagccgaaa uggacggcgc caaaggcaga cucagcuccg     1500

gccaccuaaa gugccgccuc aaaauggaca aacuaagacu gaaaggcguc agcuacagcc     1560

uaugcacagc agcauucaca uucaccaaaa uacccgccga aacccugcac ggaacaguga     1620

caguagaagu ccaauacgcc ggcaccgacg ggcccugcaa aguaccagcc caaauggccg     1680

ucgacaugca aacgcucacc ccagucggcc gacugaucac cgccaaccca gucaucaccg     1740

aauccacaga gaacucaaaa augaugcucg aacuagaucc cccauucggc gacuccuaca     1800

uaguuaucgg cguuggagaa aagaaaauca cgcaccacug gcacaggagc gguucaacaa     1860

uaggaaaagc cuucgaagcg acaguccgcg gagccaaacg aauggcggua cugggcgaca     1920

cugcauggga cuucggcagc guaggcggcg cucucaacuc ccugggaaaa ggaauacauc     1980

aaauauucgg agccgcauuu aaaucacugu ucggaggcau gucaugguuc agccagaucc     2040

uaauaggcac ccuccucaug uggcucggac uaaacacgaa aaauggaagc auaagccuua     2100

ugugccuagc acucggcgga guccuaauau uccucagcac ggccgugagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  7224
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7224
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccc       60

gaaaagaaaa aaaacgacga ggcgccgaca cauccguagg aauagucggc cuccuacuaa      120

ccacggcuau ggcagccgaa guaacgcgcc gaggaucagc cuacuacaug uacuuagaca      180

gaaacgacgc aggagaagca aucagcuucc caaccacgcu cggaaugaau aaaugcuaca      240

uccaaauaau ggaccuaggc cacaugugug acgccacuau guccuacgaa ugccccaugc      300

uagacgaggg cgucgagcca gacgacgucg auugcuggug caacaccacc agcaccuggg      360

ucguguacgg gacaugccac cacaaaaaag gcgaagcacg acgaucccgc cgagcaguca      420

cccugccauc ccauagcaca agaaaacucc aaacccgcag ccagacaugg cuagaaagcc      480

gagaauacac aaaacaccuc auccgagugg aaaacuggau cuuucgcaac ccaggcuucg      540

cacucgcagc agccgccauc gccuggcucc uaggcucgag caccagccaa aaagucauau      600

accucgucau gauacuacuc auagccccgg cauacuccau ccgaugcauu ggagucagca      660

accgggacuu cgucgaagga augagcggcg gcaccugggu agacaucgug cucgaacacg      720

gaggcugcgu aaccgucaug gcgcaggaca aacccacagu agauaucgaa cuagucacca      780

cuacaguaag caacauggcg gaaguacgaa gcuacuguua cgaggccagc auauccgaca      840

uggcaucgga cagcagaugc cccacccaag gggaggcaua ccucgacaaa caaucagaca      900

cgcaauacgu augcaaacgg acccuggucg aucgcggaug gggcaacggg ugcggccucu      960

ucggcaaagg cucacucguc accugcgcca aauucgcaug cuccaagaaa augaccggca     1020

aaagcauaca accagagaac cuagaauacc gaaucaugcu gucaguccac gguagccaac     1080

acagcggcau gaucgucaac gacaccggac acgaaaccga cgaaaauaga gccaaaguag     1140

aaaucacgcc caacagccca agagcagaag ccacccucgg cggguucggu agccuaggcc     1200

uugacugcga gccgcgcaca ggacuggacu ucagcgaccu cuacuaccug accaugaaca     1260

auaaacacug gcucguacac aaagaguggu uccacgacau cccccuaccc uggcacgcag     1320

gagccgacac cggcacgcca cauuggaaca acaaagaagc ccucgucgaa uucaaagacg     1380

cgcaugcgaa aagacagaca gucguagucc uaggaucaca agaaggagcg guacauacag     1440

cacuagcagg agcgcucgaa gcagaaaugg acggcgcaaa aggacgacua ucgucaggcc     1500

accucaaaug ccgacucaaa auggacaaac uaagacucaa aggggucagc uacuccuuau     1560

gcaccgccgc auucacauuc accaaaaucc ccgcagaaac cuuacacgga accgugaccg     1620

ucgaaguaca auacgcagga accgauggac ccugcaaagu cccagcacag auggccguag     1680

auaugcaaac ccugacaccg gucggccgac uaauaacagc aaaccccgua aucacugaau     1740

caaccgaaaa cucaaaaaug augcuagaac uugacccacc cuucggcgac agcuacaucg     1800

uaaucggcgu aggcgagaaa aaaauuacac accacuggca ccgauccgga agcacgaucg     1860

gcaaggcauu cgaagcgacg guccgaggag ccaaacgaau ggcaguccuc ggcgacacag     1920

cgugggacuu cggcagcguc ggcggcgcuc ucaacagccu aggcaaaggc auccaccaga     1980

ucuucggcgc cgccuucaaa ucccuauucg gcggcauguc augguucagc caaauccuaa     2040

uaggcacacu ccucaugugg cucggccuca acacaaaaaa cggcucaaua agccuaaugu     2100

gccucgcccu cggcggcguu cucauuuucc ucagcaccgc agucagcgcg gacugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  7225
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7225
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

aagaaaagaa acgccgaggc gcagacacga gcguaggaau agucggccua cuccugacga      120

cagcaauggc agcagaagua acacgccgug gaagcgcgua cuacauguac cucgaccgga      180

acgacgcagg agaagccauc agcuucccaa ccacccucgg aaugaacaaa ugcuacauac      240

aaauaaugga ccuaggacac augugcgacg ccaccauguc cuacgagugc cccaugcuag      300

acgaaggagu agaaccagac gacguagacu gcuggugcaa cacaacaucc acguggguag      360

uauacggaac augccaccac aagaaaggcg aagcacgccg gagccgacga gccgucacgc      420

ugccauccca cagcacacga aaacuacaaa cccgguccca gaccuggcua gaaucaagag      480

aauacacaaa acaccucaua cggguagaga acuggauauu cagaaacccc ggguucgcac      540

ucgcagccgc cgcaauagcc uggcuccugg gcucaagcac cucccaaaaa gugauauacc      600

uagucaugau acugcugauc gccccggccu acuccaucag augcaucggg gucagcaaca      660

gagacuucgu cgaagguaug ucuggaggaa ccuggguaga cauaguacua gaacacggag      720

gaugcgucac ggucauggcu caggacaaac ccaccguaga uauagaacug gucaccacaa      780

cgguaagcaa uauggccgaa guaagauccu acugcuacga ggccagcaua ucagacaugg      840

cauccgacuc acgcugccca acccaaggag aagcauaccu cgacaaacaa agcgauacgc      900

aauacgugug caaaagaacc cuaguagacc gaggaugggg aaacggcugc ggccuguucg      960

gcaaaggguc acugguaacc ugugcgaaau ucgcgugcuc aaagaaaaug acagggaaau     1020

ccauccaacc cgaaaaccuc gaguaccgaa uaaugcuaag cguccacggg ucacaacacu     1080

ccggaaugau cgucaacgac acaggacacg aaacagacga aaacagagca aaaguagaaa     1140

ucacaccgaa cagcccccgu gcugaggcaa cacucggggg auucgggucc cuaggacucg     1200

acugcgaacc acgaacggga cuagacuuca gcgaccuaua cuaucucacg augaacaaca     1260

aacacuggcu agugcacaaa gaaugguucc acgacauccc ccuacccugg cacgcaggag     1320

ccgacacugg cacaccccac uggaacaaca aagaagcacu gguagaguuc aaagacgcac     1380

acgccaagag acagaccgua gucguacucg gaagccaaga gggggcggug cacacagcac     1440

ucgcaggagc gcucgaagca gagauggacg gggccaaagg ccgacuaucc agcggacacc     1500

ucaaaugccg acuaaaaaug gacaaacuca gacuaaaagg agucuccuac agccuaugca     1560

cagcagccuu caccuucacc aagauacccg cagaaacacu ccacggcacc gucaccgucg     1620

aaguacaaua cgccgggacc gauggaccau gcaaaguccc agcgcaaaug gcaguggaca     1680

ugcaaacccu aaccccaguc ggacgucuga ucaccgcaaa ucccgucauc acagaaucca     1740

ccgaaaauag caaaaugaug cuggaacugg acccacccuu cggcgacagu uacauaguca     1800

ucggagucgg agaaaagaaa auaacacacc auuggcacag guccgggagc accauaggca     1860

aagcauucga agcaacagua cgaggagcca aaagaauggc cgucuuggga gacacugcau     1920

gggacuucgg cuccguagga ggagcacuca acagccucgg aaaaggaauc caccagauau     1980

ucggcgcagc auucaaaucc cucuucggag gcaugagcug guucucccaa auacuuauag     2040

gcacgcuccu aauguggcua ggccucaaua ccaaaaacgg aagcaucucu cuaaugugcc     2100

ucgcgcuagg aggcguccua auauuccuau caacagcagu uucagcagac ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  7226
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7226
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaag       60

aaaagaaacg ccgaggggcg gacacaagcg uggguaucgu cggccuacuc uuaaccaccg      120

cgauggccgc ggaggucacc cgacgaggcu ccgcguacua cauguacuua gaccgaaaug      180

acgccggcga agccauauca uuccccacca cgcucggcau gaacaaaugc uacauccaaa      240

uaauggaccu cggccacaug ugcgacgcca ccaugaguua ugaaugcccc augcucgacg      300

aagggguaga gccagacgac guagacuguu ggugcaacac aaccagcaca ugggucgugu      360

acggaaccug ccaccacaag aaaggcgaag cccggcgcag ccgccgggca gucacacucc      420

ccagccauag cacccgcaaa cuacagaccc gcucgcaaac cuggcucgaa ucaagagaau      480

acaccaaaca ccucauccgc gucgaaaacu ggauauuucg aaaccccgga uucgcccugg      540

cagcagcagc aauagccugg cuucucggcu ccagcacaag ccagaaaguc aucuaccucg      600

uaaugauccu auuaauagcc ccagcuuacu ccauccggug uauagggguc uccaacagag      660

acuucgucga aggcaugucc gguggaaccu ggguagacau cguacucgaa cacggaggau      720

gcgucaccgu cauggcccaa gacaaaccca ccguggacau cgaacucguc acaaccacag      780

ucucgaacau ggccgaaguc agguccuacu gcuacgaagc gucaauauca gacauggcau      840

ccgacagccg augcccaacc cagggagaag cauaccuuga caaacaaucg gacacccaau      900

acgucugcaa gcgcacacuc guggacagag gauggggcaa cggaugcgga cuauuuggca      960

aagggagccu aguaaccugc gccaaauucg cgugcagcaa gaaaaugacu ggcaaaagua     1020

uccaaccaga gaacuuagaa uaucgaauca ugcucagcgu ccacggaagc caacacuccg     1080

gcaugaucgu caacgacaca ggccacgaga cagacgaaaa ccgugccaaa guagaaauaa     1140

caccaaauag cccccgagca gaggcaaccc ucggaggcuu cgggagccua ggacucgacu     1200

gcgagccgag gacgggacua gauuucagcg accuauacua ccuaacaaug aacaacaaac     1260

auuggcuugu ccacaaagaa ugguuccacg acaucccacu ccccuggcac gccggagccg     1320

acacuggcac accucacugg aacaacaaag aagcacucgu agaguucaag gacgcccacg     1380

cuaaacgcca aacaguugua guacucggca gccaagaagg agccguccac acagcacucg     1440

caggcgcccu cgaagccgaa auggauggag ccaagggccg acuaucguca ggacaccuca     1500

agugccggcu caagauggac aaacucagac ugaaaggagu aagcuacagc cuaugcaccg     1560

ccgccuucac cuucaccaag auccccgcag aaacacucca cggaacaguc accgucgaag     1620

uacaguacgc cggaaccgac ggccccugca aaguccccgc acaaauggcc guggacaugc     1680

agacccuaac accaguagga cgccucauaa cagcaaaucc aguaauaacc gagucaaccg     1740

aaaacagcaa aaugaugcua gaacuagauc cacccuucgg cgacuccuac auaguaaucg     1800

gcguaggcga gaagaaaaua acacaucacu ggcacagauc agguuccaca aucgggaaag     1860

cauucgaagc cacaguccgc ggagccaaaa gaauggcggu acuaggcgac acagccuggg     1920

acuucggaag uguagguggc gcgcucaacu cccuagggaa gggaauacac caaaucuucg     1980

gcgccgcauu caaaagccua uucggcggaa ugagcugguu cagccaaauu cucauaggca     2040

cccuccuaau guggcuugga cucaauacaa agaacgggag cauaucccua augugccucg     2100

cccuaggcgg cguccuaaua uuucugagca cagcagucag cgcagacuga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  7227
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7227
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaaa       60

agaagaggcg aggagccgac accagcguag ggauaguagg ccuacuccuc acaacagcua      120

uggcagcaga agucacacga cgcggcagug cauacuacau guaccucgac cgaaacgaug      180

ccggcgaggc caucagcuuc cccaccacac uaggcaugaa caaaugcuac auacaaauca      240

uggaccucgg acacaugugc gacgcaacaa uguccuacga augcccaaug cucgacgagg      300

ggguagaacc cgacgacgua gacugcuggu gcaacacgac cuccacaugg gugguuuacg      360

gcacaugcca ccacaagaaa ggcgaagccc gacgaagccg acgugcagug acacucccau      420

cgcacuccac acgaaaacua caaacacgau cucaaacaug gcuagaaucc cgcgaauaca      480

caaaacaccu aauacgggug gaaaacugga uauucagaaa ccccggcuuc gcccuagcag      540

cagccgcaau agcauggcuc uuagggagcu ccaccucaca aaaagucauc uaccucguaa      600

ugaucuuacu aauagcgccg gccuacagca uacgcugcau agggguaagc aaccgagacu      660

ucgucgaagg aaugagcgga ggcaccuggg uagauaucgu acuagagcac ggcggcugcg      720

ucacuguaau ggcacaagac aaaccuacag uagacaucga auugguaaca acaacaguca      780

gcaacauggc cgaagucaga ucauacugcu acgaagcgag cauaagcgac auggcaucug      840

acagccgcug ccccacccag ggcgaagccu accucgacaa acagucagac acccaauacg      900

uaugcaaaag aacacucgua gaccgaggau ggggcaaugg augcggacuc uucgggaaag      960

gaagccuagu cacaugcgcc aaauuugcau gcucaaagaa aaugacagga aaaucgaucc     1020

aaccagaaaa cuuagaauau cgaaucaugc ugagcguaca cggcagccaa cacagcggca     1080

ugauagucaa cgacacugga cacgaaaccg acgaaaaccg ggcaaaaguc gaaaucaccc     1140

caaacucacc aagggccgaa gcaacacucg gcggcuuugg aagccuaggc cucgacugcg     1200

aaccccgcac cggacuagac uucucagacc uauacuaccu aaccaugaac aacaaacacu     1260

ggcuaguuca caaggaaugg uuccacgaca uaccccuccc auggcacgcc ggcgcagaca     1320

caggaacgcc ccacuggaac aacaaagaag cacucguaga guucaaggac gcccacgcca     1380

agcggcagac aguagucgug cucggaucac aagaaggagc cguccacaca gcccucgccg     1440

gagcccuuga agcggaaaug gacggcgcaa aaggacggcu gucuagugga caccuaaaau     1500

gucggcugaa aauggacaaa cugcgucuaa aagguguaag cuacagccua ugcacagcag     1560

cguucaccuu caccaaaauc ccggcagaaa cgcuccacgg aaccgucaca guagaaguac     1620

aauacgcagg gacagacggc ccaugcaaag uacccgccca aauggccgua gacaugcaaa     1680

cacuaacacc agucggaaga cucauaacag ccaaccccgu aaucacagaa agcaccgaga     1740

acuccaaaau gaugcuggaa cucgauccuc ccuucggcga cagcuauaua gucauaggug     1800

ucggugagaa gaaaauaaca caccacuggc accgcagcgg cagcacaauc ggaaaagccu     1860

ucgaagcaac cguucgggga gcgaaacgaa uggccgucuu aggcgacaca gcaugggacu     1920

ucggcucugu aggcggagca cugaacagcc uaggcaaagg aauccaccag auauucggcg     1980

cagccuucaa aucacucuuc ggcgggaugu cuugguucuc acaaauacuc auaggaacac     2040

uccucaugug gcuaggcuua aacaccaaaa acggaucgau cucgcugaug ugccuagcac     2100

ucggcggagu gcugauauuc cuaucgaccg caguaucggc cgacugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  7228
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7228
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aacgccgcgg cgccgacacc agcguaggaa ucgucggacu acuguugaca accgcaaugg      120

ccgcggaagu gacacgaagg ggaagcgccu acuacaugua ccucgacaga aacgacgccg      180

gagaagccau auccuucccg accacacuag gcaugaacaa augcuacaua caaauaaugg      240

acuuaggcca uaugugcgac gcaacgaugu cauacgaaug cccuaugcua gacgaaggag      300

uggaacccga cgacgucgac ugcuggugca acacgacauc gaccugggua gucuacggca      360

cuugccacca caagaaagga gaagcacgac gaagccgacg ggcagucacg cuacccagcc      420

acuccacccg aaaacuccaa acucgcagcc aaaccuggcu cgaaagccgc gaauacacca      480

agcaccuaau acgagucgaa aacuggauau uccggaaccc aggcuucgcc cucgcagcag      540

cagcaauagc cuggcuccuu ggaagcucca caucccaaaa aguaauauac cuagucauga      600

uacuccuaau cgcccccgca uacagcaucc gauguaucgg aguauccaac cgcgacuucg      660

ucgagggcau gagcggcggc accuggguag acauaguccu cgaacacgga ggcugcguca      720

caguaauggc ccaagacaaa cccacaguag acaucgagcu ggucaccaca acgguaagca      780

acauggccga aguccgaucc uacugcuacg aagcaagcau aucagacaug gcaucagaca      840

gcagaugccc cacacaaggc gaagccuacc uagacaaaca aagcgacaca caauacguau      900

gcaaacggac guuggucgac cggggcuggg gcaacggaug cggucucuuc ggaaaaggca      960

gccucguaac cugcgccaaa uucgcgugcu caaagaaaau gacgggcaaa ucaauccaac     1020

cggaaaaucu agaguaccgg auaaugcuaa gcguccacgg aucacagcac uccggcauga     1080

ucgucaacga caccggacac gaaacagacg aaaaccgugc aaaaguggag aucacaccaa     1140

acagcccccg cgccgaagcc acacuaggcg gauucggauc ccugggacua gacugcgaac     1200

caaggacagg acuagacuuc uccgaccucu acuaccucac aaugaauaac aaacacuggc     1260

uaguacacaa agaaugguuc cacgacaucc cacuccccug gcacgccgga gcagacacag     1320

gcacaccaca cuggaacaac aaagaagcac ucgucgaguu caaagacgca caugccaaac     1380

gccaaaccgu cguagugcua ggaucccaag aaggagcagu ccacaccgcg cucgccggag     1440

cccuggaagc cgaaauggac ggagcaaaag ggcgccugag uuccggccac cuaaagugcc     1500

gccuaaagau ggacaaacua agacucaaag gcgucuccua cagccucugc accgcagcgu     1560

ucaccuucac uaaaaucccg gccgaaacac ugcacggaac aguaaccgug gaggugcagu     1620

acgcaggcac agacggaccc ugcaaagugc cggcccaaau ggcagucgau augcaaacac     1680

uaacgcccgu aggacgacuc aucacagcca accccguaau cacagagucc acagaaaacu     1740

caaaaaugau gcuagaacua gaucccccau ucggagacag cuacauaguc aucggcgucg     1800

gugaaaagaa aauaacacac cauuggcaca gaagcggcuc aaccaucgga aaagccuucg     1860

aagccacggu ccgaggggca aaacgcaugg ccguccuagg ggacacggca ugggacuucg     1920

ggagcguagg gggagcccuc aacucccugg gcaagggaau acaccaaaua uucggcgccg     1980

cuuucaaaag ccucuucggc gggaugagcu gguucagcca aauccucauc ggaacacuuc     2040

ucauguggcu cggucuaaac accaaaaacg gaagcauauc gcugaugugc cucgcgcuug     2100

gcggcguccu uauauuccua ucaaccgccg uuuccgccga cugaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  7229
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7229
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagc       60

gccgcggagc cgacacaagc guaggaauug ucggccuccu ucuuacaaca gcgauggcgg      120

ccgaaguaac ccgaagaggc uccgccuacu auauguaccu cgacaggaac gacgccggcg      180

aagcaaucuc auuccccaca acccugggaa ugaacaaaug cuacauccag auuauggacc      240

uaggccacau gugcgaugcc accaugucgu acgaaugccc caugcuagac gaaggaguag      300

aaccagacga cguagacugc uggugcaaca caaccagcac augggucgua uacggaacau      360

gccaccacaa aaagggcgaa gcgcgaagaa gccgccgcgc agugacccuc ccaagccaca      420

gcacacgaaa acuccaaaca cgcagccaaa cauggcucga aagccgggaa uacaccaaac      480

accucaucag ggucgaaaac uggaucuucc gaaaccccgg cuucgcacuc gcugcagcag      540

caauagccug gcuucucgga uccagcaccu cccaaaaagu cauauaccua gucaugauac      600

uccuaauagc ccccgcauac ucuauacgcu gcauaggagu gaguaaccga gacuucgugg      660

aagggauguc cggcggaaca ugggucgaca ucguccuaga acacgguggc ugcguaacag      720

uaauggcaca agacaaaccc acaguggaca ucgagcuugu aacaaccaca guauccaaca      780

uggcagaagu cagauccuac ugcuacgaag ccuccauauc ugacauggcc ucagacucac      840

gaugcccaac ccaaggagaa gcauaccuag acaaacaaag cgacacacaa uauguaugca      900

aaagaacauu agucgaccgc ggauggggaa acggaugcgg acuuuucgga aaagguuccc      960

uagugacuug cgccaaauuc gccuguucga agaaaaugac cggcaagucc auccaaccgg     1020

aaaaccugga auaccgaauc augcuaucug uccacggcuc gcaacacuca ggcaugaucg     1080

uaaacgacac gggccacgaa acagacgaaa acagagccaa agucgagaua acccccaacu     1140

ccccucgcgc cgaagccacg cucggcggau ucggaagccu gggccucgac ugugaaccgc     1200

gaacaggacu agacuucagc gaccucuacu accuaaccau gaacaacaag cacuggcuag     1260

uccacaaaga augguuccac gacaucccac ucccauggca cgcaggagcc gacaccggaa     1320

caccacacug gaacaacaag gaagcccucg ucgaguucaa agacgcgcac gccaaacgcc     1380

aaacaguggu cguccuagga ucccaagaag gcgccgucca caccgcacua gcgggagccc     1440

ucgaagccga aauggacggc gcaaaaggaa gacucagcuc gggccaccua aaaugcagac     1500

uaaaaaugga uaagcuccga cugaaaggag uaucuuacuc ccuaugcaca gcagcauuca     1560

ccuuuacuaa aauccccgca gaaacacugc acggaacagu aaccgucgag guacaauacg     1620

caggcaccga cggacccugc aaaguaccgg cccaaauggc cgucgacaug caaacccuca     1680

caccagucgg aagacucauc acggcaaacc ccguaaucac cgaauccacc gaaaacucua     1740

aaaugaugcu ggagcuagac ccacccuucg gcgacagcua caucgucauc ggagugggag     1800

agaagaagau cacccaccac uggcaccgaa gcggcagcac caucggcaaa gcauucgagg     1860

cgacuguccg cggagcgaaa agaauggccg uucucgggga caccgcaugg gacuucgggu     1920

ccgucggcgg cgccuuaaac agucucggaa aaggcauaca ccaaauauuc ggcgccgcgu     1980

ucaaaagccu guucggaggc auguccuggu ucucgcaaau acuaaucggc acccuccuua     2040

uguggcucgg gcuaaacacc aaaaacggaa gcauaagccu aaugugccua gcccuaggcg     2100

gcgugcucau cuuccuuagc acagccguaa gcgcggacug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  7230
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7230
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

ggggcgcaga cacaagcguc ggaaucgucg gacuccuacu uacgaccgca auggcggcug      120

aagucacacg ccgaggaucc gcauacuaca uguaccucga ccguaacgac gcaggcgaag      180

ccauaucauu cccuacaacg cugggaauga acaaaugcua cauccagaua auggaccuag      240

gccacaugug cgacgcaacc auguccuacg aaugcccaau gcucgacgaa ggggucgaac      300

ccgacgacgu agacugcugg ugcaacacaa cgagcacaug gguaguguac ggaacaugcc      360

accacaaaaa gggggaagcc cgacgaucca ggagagcagu gacacuccca agccacagca      420

caagaaaacu acagacaaga ucacaaaccu ggcuugaaag cagagaauac acaaagcacc      480

uaauacgagu ugaaaacugg auauucagaa accccggcuu cgcacucgca gcagccgcca      540

uagccuggcu ccugggcagu ucaacgagcc aaaaagucau auaccuagua augauacugc      600

uaaucgcccc agcauacuca auacgcugca ucggcguauc caaccgagac uucguagaag      660

gaaugucagg aggaaccugg guagacauug uacucgaaca cggaggaugc guaacaguca      720

uggcccagga caaacccacg gucgacaucg aacucguaac aacaaccgua ucaaacaugg      780

cagaaguacg auccuacugc uacgaagcaa gcaucucuga cauggccuca gacucacgau      840

gccccaccca gggcgaagcc uaccuagaca aacagucgga cacacaauac gucugcaaac      900

gaacacuggu cgaccgcggu uggggcaacg gcugcggccu guucggaaaa gggagccuag      960

ugaccugcgc caaauucgcc ugcagcaaga aaaugaccgg caaaucgaua caacccgaaa     1020

accuugagua cagaaucaug cuauccgucc acggcagcca acauuccggc augauaguca     1080

acgacaccgg ccacgaaaca gacgaaaaca gagcaaaagu cgaaaucacg cccaacuccc     1140

cacgggcgga agccacacug ggaggauucg gcagccucgg ccuagauugc gaaccacgca     1200

caggguuaga cuucuccgac cucuacuacc uuacaaugaa caacaaacau uggcucgugc     1260

acaaagaaug guuccaugac aucccacucc ccuggcaugc aggcgccgac acggggacac     1320

cacacuggaa uaacaaggag gcgcuugugg aguucaagga ugcgcacgcc aaacgacaaa     1380

caguaguugu ccuaggcuca caagaaggcg cgguccacac agcccucgcc ggggcacuag     1440

aagccgaaau ggacggcgcc aaagggcgac ucagcagcgg ccaccucaaa ugccgacuaa     1500

aaauggacaa acugcgccua aaagggguca gcuacagccu cugcaccgcc gcauucaccu     1560

ucacaaaaau accagccgaa acccuacacg gcacagucac aguagaagua caauacgccg     1620

gcacagacgg acccugcaaa gucccagccc aaauggcggu cgacaugcaa acccucacgc     1680

caguaggccg acuaaucaca gcaaacccag ucaucaccga gagcacagaa aacagcaaaa     1740

ugaugcuaga acuggacccg ccauuuggag auaguuacau cguaauuggc gugggggaaa     1800

agaaaauaac acaccacugg cacagaagcg gcuccacaau cggaaaagca uucgaagcua     1860

ccguccgagg agcgaaacga auggccguac ucggcgacac agccugggac uucggcucag     1920

uaggcggagc ccugaacucc cucggcaaag gaauacacca aaucuucggg gccgcguuca     1980

aaucccuguu cggaggaaug agcugguucu cacagauacu cauaggcaca cuacuaaugu     2040

ggcuaggccu aaacacaaag aacggcagca uuagcuugau gugccuagcc uugggaggcg     2100

uacuaauauu ccuaagcaca gccguaucag ccgacugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  7231
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7231
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gagccgauac uucggucggc auagucggcc uacuccuaac cacggccaug gcugcagaag      120

ucacaagacg gggcucagcc uacuacaugu accucgacag aaacgaugca ggagaagcaa      180

ucucguuccc cacaacacug ggcaugaaca aaugcuauau acaaaucaug gaucuuggcc      240

acaugugcga cgcaaccaug agcuacgagu gcccaaugcu cgacgaaggc gucgaacccg      300

augacgugga cugcuggugc aacacaacca gcacaugggu cguauacgga accugccacc      360

acaagaaagg cgaagcccgc cgaucacggc gcgcagugac ccuccccuca cacuccaccc      420

gaaaacuaca aacaagaagc caaaccuggc ucgaaucacg cgaguacacc aaacaccuaa      480

ucagaguaga aaacuggaua uuccgcaacc caggcuucgc acuggccgcc gcggcaauag      540

cauggcuccu uggcucaagc acaucacaaa aaguaauuua ccucguaaug auacuacuca      600

ucgcccccgc cuauagcauc cgcugcaucg gcgucucaaa ucgcgacuuc guagaaggca      660

ugagcggcgg aacaugggua gacaucgucc ucgaacacgg agguugcguc acggucaugg      720

cccaagacaa acccacagua gacaucgagc uagucaccac gacagucucu aacauggcug      780

aaguacgaag cuauugcuac gaagcgagua ucuccgacau ggcaucagac ucacguugcc      840

ccacccaagg agaggccuac cucgacaaac aauccgacac acaauacguc ugcaaacgca      900

cccucguaga cagaggaugg gggaacggcu gcggacuguu cggcaaagga agccucguaa      960

caugcgcaaa auucgcaugc ucaaagaaaa ugaccggaaa aucaauacaa ccagaaaacc     1020

ucgaauaccg cauaaugcua uccguacacg gcucccaaca cuccggaaug auaguaaacg     1080

acaccggcca cgaaaccgac gagaaccgag caaaagucga gaucaccccu aacuccccac     1140

gcgcagaagc aacccuaggc ggcuucggcu cccucggacu ggacugcgaa cccagaaccg     1200

gacuggacuu cucagaccuc uacuaccuca caaugaacaa caaacacugg cucguucaca     1260

aggagugguu ccacgacauc ccccuccccu ggcacgcagg cgcggacaca gguacgccac     1320

acuggaacaa uaaggaggca cucgucgagu ucaaagacgc acacgcaaaa cgccaaacug     1380

ucgucguacu gggcucccaa gaaggagccg uacacacagc ccucgccggg gcacucgaag     1440

ccgaaaugga cggcgccaag ggacgacuaa gcuccggcca ccucaaaugc cgacuaaaaa     1500

uggacaagcu acggcucaaa ggcgucucgu acagccucug cacagcggcc uucaccuuca     1560

ccaaaauccc cgccgaaacu cuacacggca cagucaccgu agaaguccag uacgcaggaa     1620

ccgacggccc cugcaaggug ccagcacaaa uggccguaga uaugcaaacc cuuacccccg     1680

uaggccgccu cauuaccgcc aaccccguga uaaccgagag caccgaaaac agcaaaauga     1740

ugcuagaacu cgauccccca uucggcgaca guuacaucgu caucggcguc ggagagaaga     1800

aaaucacaca ccacuggcac agauccgguu caacaauagg aaaagccuuc gaagcaaccg     1860

uccgcggagc caaacgcaug gcaguacucg gcgacaccgc augggacuuc ggauccgucg     1920

gaggagcccu aaacucccuc ggcaagggaa uacaccaaau cuucggcgcc gccuucaaaa     1980

gccucuucgg uggaauguca ugguucagcc aaauccugau cgggacacuu cuaauguggc     2040

uuggccuaaa caccaaaaac ggcagcauca gucucaugug ucuagcccuc ggcggaguac     2100

ucauauuccu aucaacagca guauccgccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  7232
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7232
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggggg       60

cggauaccuc cguaggaaua gucggacuac uacucacuac cgcaauggcc gcagagguaa      120

cacgacgcgg cagcgccuac uacauguacc uagaccgaaa cgacgcagga gaagcaauca      180

gcuuccccac gacacuggga augaacaagu gcuacauaca gaucauggac cucggccaca      240

ugugcgacgc aaccaugagc uacgaaugcc caaugcuaga cgaaggagua gaacccgacg      300

acguagacug uugguguaac accacaagca ccugggucgu cuacggaaca ugccaccaca      360

agaagggaga ggcccgccgc agcagacgag ccguaacacu acccucgcac agcaccagaa      420

aacuacagac cagaagccaa acauggcuag aaagcagaga auacacaaaa caccuaaucc      480

ggguagaaaa cuggauauuu aggaacccgg gauucgcacu agcagccgca gcaauagcau      540

ggcuacuagg gagcucaacc ucccaaaaag uaauauaccu agucaugauc cuacucaucg      600

cgccagcaua cuccauccgg ugcauaggag uaucaaacag agauuucgua gaaggcaugu      660

cagggggcac cuggguagac auaguccucg aacacggagg gugcguaacg gugauggcac      720

aagauaaacc aaccguagac aucgagcuag uaacaacaac agucagcaac auggccgaag      780

uaaggucgua cugcuacgaa gccaguaucu cagacauggc cagcgacuca cgaugcccaa      840

cccaaggaga agccuaccua gacaaacagu ccgacacaca guacguaugc aaacgcacgc      900

ucgucgaccg aggcugggga aacggcugcg gccuguucgg aaaagggagc cuggucacau      960

gcgcaaaauu cgccugcucc aagaaaauga ccggaaaauc cauacaaccc gaaaaccuag     1020

aauaccggau caugcucagu guccacggca gucagcauag cggcaugauc guaaacgaca     1080

ccggacacga aacagaugaa aaccgagcaa aagucgaaau aaccccaaac ucgccccgag     1140

cagaagccac auuaggcggc uucggauccc uaggacuaga cugcgaaccc cgaaccggac     1200

ucgacuucuc agaccuguau uaucucacga ugaacaacaa acacuggcug guacacaagg     1260

aaugguucca cgacauacca cuaccauggc acgcuggagc ugacaccgga acaccacacu     1320

ggaacaacaa agaggcccua guagaguuca aagaugcaca ugccaaacgc caaaccgugg     1380

uaguccuagg gucccaagaa ggagccgugc acaccgcacu cgccggcgca cuggaagcag     1440

aaauggacgg agccaaaggg cgacuaagcu ccggccaccu aaaaugcaga cucaaaaugg     1500

acaaauuacg acuuaaaggc guaagcuauu cacugugcac ggcggcauuc acauucacca     1560

aaauacccgc cgaaacccua cacggaacag ucaccguaga aguccaauac gccgggacag     1620

acggaccgug caaaguccca gcccaaaugg ccguagacau gcaaacacuc acccccguag     1680

gaagacucau aaccgcaaac ccggucauca ccgaaucaac cgaaaacagc aaaaugaugc     1740

ucgaacuaga cccgcccuuc ggggacagcu auauagugau aggcguagga gaaaagaaaa     1800

uaacgcacca cuggcaccga agcggcagca caauagggaa agccuucgaa gccacaguac     1860

gaggagcgaa aagaauggca guccugggag acaccgcgug ggacuucgga ucgguaggag     1920

gcgcacuaaa cagccuggga aaaggaauac accaaaucuu cggcgccgcc uucaaaucac     1980

ucuucggcgg aaugucaugg uucucccaga uacuaaucgg gacccuccug auguggcuag     2040

gucuaaacac caaaaauggc ucaaucucac uaaugugccu agcgcuagga ggcguccuca     2100

ucuuccuguc cacagccgua ucggccgacu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  7233
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7233
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcag       60

acaccagugu aggcaucgua ggacuccuac uaaccacagc aauggccgcu gaagucacac      120

gacgcggaag cgcauacuac auguaccuag accguaacga cgccggcgaa gcaaucucgu      180

uccccacgac ccucggaaug aacaaaugcu acauucaaau uauggaccua gggcacaugu      240

gcgacgcgac aaugagcuac gaaugcccca ugcucgacga aggcguggaa ccagacgacg      300

ucgacugcug gugcaacaca acaagcacau ggguggucua uggaaccugc caucacaaga      360

aaggcgaagc acgaagaagc cgccgggcag uaacacuucc gagucacagc acccgaaaac      420

uccaaacacg cagccaaaca uggcuagaaa gccgcgaaua uacgaaacac cuaaucagag      480

uagaaaacug gauauuccga aacccuggcu uugcacucgc cgcagcagcc auagcauggc      540

uccugggcag cagcacaagc caaaaaguca ucuaccuagu caugauacuc cuuaucgccc      600

ccgcauacuc aauccgcugc aucggcgucu cgaacaggga cuucguggag ggcaugagug      660

ggggaaccug gguagacauc guccuagaac acggcggcug cgucacggua auggcacagg      720

acaaaccaac aguagauaua gagcuaguaa ccacuacagu auccaacaug gcagaagucc      780

gcagcuacug cuacgaagca ucgauaucgg acauggccag cgauuccaga ugcccaaccc      840

aaggggaggc cuaccucgac aaacaaagcg acacccagua cguaugcaaa agaacacucg      900

uggacagagg guggggcaac ggcugcggac uauucggcaa gggaucacug guaaccugcg      960

ccaaauucgc cugcuccaag aaaaugaccg gcaaaagcau acagccagaa aaccuagaau     1020

acagaaucau gcucuccgua cacggcucgc aacacagcgg aaugaucgua aacgacacag     1080

gacacgaaac agacgaaaac cgcgcaaaag ucgaaaucac cccgaacucc ccgcgcgcag     1140

aagcaacacu aggcggcuuc ggaagccucg gacuagacug cgaaccucga accggccucg     1200

acuucaguga ccucuacuac cucacaauga acaacaaaca cuggcuagua cacaaagaau     1260

gguuccacga caucccccua cccuggcacg cgggcgcaga cacaggcaca ccacacugga     1320

acaacaaaga agcccuggug gaguucaagg acgcccacgc aaaacgacaa accgucgucg     1380

uacuugggag ucaagaaggc gcaguacaca cagcacucgc cggagcacuc gaagccgaaa     1440

uggacggugc caaaggacgc cugagcagcg gccaccucaa gugcagacua aagauggaca     1500

aacuaagacu caaaggagug agcuacagcc uuugcaccgc agccuucacc uucacaaaaa     1560

uaccggccga aacccugcac ggaacgguca ccguagaagu ccaguacgcc ggaacugacg     1620

gacccugcaa ggucccagca caaauggcgg uagacaugca aacccuaaca ccaguaggga     1680

gauuaauaac cgccaacccc guaaucacag aaagcacgga aaacagcaag augaugcuag     1740

aacucgaccc gcccuucggg gacagcuaca uaguaauagg agucggagaa aagaagauca     1800

cccaccacug gcaccgaagc gggagcacca ucgguaaagc guucgaagca acaguacgcg     1860

gcgccaaacg cauggccgua cucggugaca ccgccuggga cuucggcucg gucggcgggg     1920

cccucaauag ccuuggcaaa ggcauucacc aaaucuucgg cgccgcauuc aaaagccuau     1980

ucggcggaau gagcugguuc agccagauac ucauagguac acuucuaaug uggcucgggc     2040

ucaacaccaa aaacggcuca aucagccuaa ugugccuggc ccucggagga gugcuaaucu     2100

uccuaagcac agccgucagc gccgacugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  7234
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7234
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

caagcgucgg caucguagga cuacuacuca caacagccau ggcagcugaa gucaccagac      120

gcggcuccgc auacuacaug uacuuagacc gcaacgacgc cggcgaagcc aucucauucc      180

caacaacacu gggaaugaac aaaugcuaca uccaaaucau ggaccucgga cacaugugcg      240

acgcaacgau gucauaugag ugcccuaugc uggacgaagg agucgaacca gacgacguag      300

acugcuggug caacaccacg agcacguggg ucguauacgg cacuugccac cacaagaaag      360

gggaagcccg ccgcucacgc cgagccguaa cccuaccauc ccacucaacc cggaaacuac      420

aaacacgcuc ccaaacaugg cuagaaagca gagaguacac aaaacaccug auacgcgucg      480

aaaacuggau auuccgaaac cccggauucg cccucgccgc ugccgccaua gccuggcucc      540

ugggcagcuc caccucacag aaagucaucu aucucguaau gauccuacuc aucgcccccg      600

cauacagcau acgaugcaua ggcgucucaa accgcgacuu cgucgaagga auguccggag      660

gcaccugggu ugauaucguc cuagaacacg guggaugcgu aaccgucaug gcacaagaua      720

aacccaccgu cgacaucgaa cucguaacaa ccacagucuc gaacauggcc gaaguacgau      780

ccuacugcua cgaagccagc aucucggaca uggccuccga cagccgcugc cccacccaag      840

gcgaagcaua ccucgacaaa caaagcgaca cucaauacgu augcaagcgg acacuuguag      900

accgaggcug ggggaacggc ugcggacucu ucggcaaagg cagccuugua acgugcgcaa      960

aguucgccug cucaaagaaa augaccggaa aauccauaca acccgaaaac cuugaauacc     1020

gaauaaugcu aagcguccac ggaucgcaac auuccggaau gaucgucaac gauaccggcc     1080

acgaaaccga cgaaaaccga gcaaaagucg aaaucacacc caacagcccc cgcgcagaag     1140

ccacccuagg gggcuucgga agccucggac uagacugcga accgcgcaca ggccucgacu     1200

ucagcgaccu guacuaccuc accaugaaca auaaacacug gcuaguccau aaagaguggu     1260

uccacgauau ccccuuacca uggcacgcag gagcagacac cggcacccca cacuggaaca     1320

acaaagaggc acucgucgag uucaaagacg cacacgccaa acgccaaacc gucgucguac     1380

ucggcucaca agaaggcgcc guccauacag cccuggcggg agcccuagaa gcagaaaugg     1440

acggagccaa aggcagacua agcucgggcc accucaaaug ccgccuaaaa auggacaaac     1500

ucagacucaa aggaguaagc uacucuuuau gcaccgccgc guucacauuc acaaaaauuc     1560

ccgcagaaac ccuccacgga acaguaaccg uagaaguaca auacgccggc accgacggcc     1620

ccugcaaagu cccggcccag auggccguug acaugcaaac ccuaacaccc gucggcagac     1680

uaauaaccgc caacccagua aucaccgaau ccacggaaaa cucaaaaaug auguuagagc     1740

uagauccacc guucggagac agcuacaucg ugaucggcgu cggcgaaaag aaaauaaccc     1800

accacuggca ccgcagcgga agcaccauag gcaaagccuu cgaagccacc guccgagggg     1860

cgaaacgcau ggcgguccua ggagacacag cuugggacuu cggcagcgua ggcggagcgc     1920

uaaacucacu cggcaaaggc auccaccaaa ucuucggcgc ggcauucaaa ucguuguucg     1980

gaggaauguc augguucuca cagauacuca ucggcacacu ccugaugugg cuaggccuca     2040

acaccaaaaa uggcucaaua agccuaaugu gccuagcacu cggcggcguc cuaaucuucc     2100

ugagcacagc cgucagcgcu gacugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  7235
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7235
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggaau ugucggacuc cuacuaacaa ccgcgauggc agcagaagua acgcgacgcg      120

gcucagccua cuacauguac cuagaccgaa acgacgccgg agaagccauc ucauucccga      180

ccacacucgg gaugaauaaa uguuacauac aaauuaugga ccuaggccac augugcgacg      240

caaccauguc guacgaaugc ccgaugcucg acgagggagu cgaaccggac gacguagacu      300

gcuggugcaa caccacgucc acaugggucg ucuacgggac cugccaccac aagaaaggag      360

aagcacgacg aagccgacgg gcgguuaccc uaccaagcca cagcacacgc aaacuccaaa      420

cacgcaguca aaccuggcuc gaaucccgag aauacaccaa acaccuaaua cgcgucgaga      480

acuggaucuu caggaaucca ggauucgccc ucgcagcagc agccaucgca uggcuccugg      540

gcucgagcac uagccaaaaa guaauauacu uaguaaugau ccuacugauc gcccccgcau      600

acucaauacg augcaucggc guaagcaacc gcgacuucgu cgaaggcaug ucaggaggaa      660

cauggguaga cauaguccuc gaacacgggg gcugcgucac gguaauggca caggacaaac      720

caacagucga cauagagcua gugaccacca cagucaguaa cauggcggaa guccgaucgu      780

acugcuacga agccagcaua ucagacaugg ccucagacuc acgcugcccc acacagggag      840

aggccuaccu cgacaagcaa agcgacacac aauaugucug caaaagaaca uuagucgauc      900

gcgggugggg caacgggugc ggccucuucg gcaaaggcag ucucguaaca ugcgcaaaau      960

uugcaugcuc caagaaaaug acaggaaaau ccauacaacc cgaaaaucuc gaguacagaa     1020

ucaugcuauc cguccacggc ucccaacaua gcgggaugau agugaacgac acgggccacg     1080

aaacagacga aaaccgagcc aaagucgaaa uaacacccaa caguccccgc gcagaagcaa     1140

cacuaggcgg guucggcagc cuaggcuuag acugcgaacc acgcacaggc uuagacuuca     1200

gcgaccuaua cuaccucacc augaacaaca aacacuggcu gguccacaaa gagugguucc     1260

acgacauccc acuccccugg caugcaggag cugacacggg caccccacac uggaacaaca     1320

aagaggcucu cguagaguuc aaagacgcac acgcaaaacg acaaacaguc gugguccuag     1380

gcucacaaga aggagcggua cauaccgcac uagccggagc ccucgaggcc gaaauggacg     1440

gagcgaaggg gcgccuaagc ucaggacacc uaaagugccg acuuaaaaug gacaaacuac     1500

gauugaaagg agucuccuac ucccucugca cagcagccuu uacauuuacc aaaauaccgg     1560

ccgagacccu gcacgggacu guaacaguug agguacaaua cgccgggacc gacggcccau     1620

gcaaaguacc agcccaaaug gcaguagaca ugcagacccu aacccccgua gggcgccuga     1680

uaaccgccaa uccagucauc acagaaucaa cggaaaacuc aaaaaugaug cuagaacuag     1740

aucccccauu cggcgacagc uacauaguca uaggagucgg agaaaagaaa auaacccacc     1800

acuggcacag gucaggcuca acaaucggaa aagcauucga agccacggua cgaggcgcga     1860

aaagaauggc gguccucggc gacacugcau gggacuucgg auccguaggc ggcgcccuca     1920

acucacucgg aaaaggaauc caccaaaucu ucggcgccgc auucaaauca cuguucggcg     1980

ggauguccug guucagccaa auacucaucg gaacgcuacu aauguggcua ggccuaaaca     2040

ccaagaacgg gagcaucucc cugaugugcc ucgcccuagg aggaguacug auauuccuga     2100

gcacagccgu aagcgccgac ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  7236
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7236
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cguaggacuc cuccuaacaa ccgccauggc agccgcaguu acacgacgag      120

gaagcgccua cuacauguac cuagacagaa acgacgccgg agaagccaua uccuucccga      180

cgaccuuagg caugaacaaa ugcuacauac aaauaaugga ccuaggacac augugcgacg      240

caacgauguc auaugaaugc cccaugcucg acgaaggagu ggaaccugac gacguagacu      300

gcuggugcaa cacgacauca accuggguag uauacggaac cugccaccac aagaaaggcg      360

aagcccgacg uucccggcga gccguaaccc uccccagcca cuccacacgc aaacuacaga      420

cacgaucaca aaccuggcua gagucacgag aauacacaaa acaccugaua cgcguagaga      480

acuggaucuu ccgcaacccc ggauucgcac uagccgccgc ugccaucgca uggcuacuag      540

gcagcucgac cucacaaaaa guaauauacc ucguaaugau ccuccuaauc gccccagccu      600

acuccauacg cugcaucggg gucucaaacc gcgacuuugu cgaaggcaug uccggaggaa      660

ccugggucga cauaguacuc gaacacggcg gcuguguaac cguaauggca caagacaagc      720

cuacaguaga caucgagcua gucacaacaa caguaucaaa cauggccgaa guacgcuccu      780

acugcuacga agcaagcaua agcgacaugg caagcgacag ccggugcccc acacaaggcg      840

aagccuaucu ugacaaacag agcgacacac aauacgucug caaaaggacc cucguagaca      900

gaggcugggg caacggcugc ggacuauucg gaaaaggguc acucgucaca ugcgcaaaau      960

ucgccugcag uaagaagaug accgggaaaa gcauccaacc cgaaaaccua gaauaccgua     1020

ucaugcucag cguacacggc ucacaacacu ccggcaugau ugucaaugac accggucacg     1080

aaacagacga gaaccgcgca aaaguggaaa uaacccccaa cucgcccagg gccgaagcga     1140

cacucggagg auucggaucc cuuggccuag acugcgaacc caggacagga cucgacuucu     1200

cagaccucua cuaccuuacc augaacaaca aacacuggcu cguacacaaa gaaugguuuc     1260

acgacauccc ccuaccaugg cacgcgggag ccgacacagg cacaccacac uggaacaaca     1320

aagaagcccu agucgaauuu aaggacgcac acgcaaaaag acaaaccgua guuguccuag     1380

gaucacaaga aggcgcagua cacacggccc ucgccggagc ccucgaagcc gaaauggacg     1440

gcgccaaagg ccgacuaagc agcggccacc ucaaaugccg ccugaaaaug gacaaacucc     1500

gacuaaaagg ugucaguuau ucccuaugca cagccgccuu uaccuucaca aaaauacccg     1560

cagaaacccu ccacggcacc gucaccgucg agguccaaua cgccggcacg gacggccccu     1620

gcaagguacc cgcacaaaug gccguggaca ugcaaacccu gacccccgug ggcagacuca     1680

uaacagcaaa cccagucaua acagaaagca cagaaaacuc caaaaugaug cucgaacuag     1740

acccgcccuu cggggacuca uacauaguaa uaggcguagg agagaagaaa aucacccacc     1800

acuggcaccg aucgggaucu accaucggca aagcauucga agccaccgug cgaggcgcca     1860

aacgaauggc aguccucggc gacaccgcau gggacuuugg cucugucgga ggagcccuaa     1920

acagccucgg caaaggcauc caccaaaucu ucggugcagc guucaaaagc cucuucggcg     1980

gcauguccug guucagccaa auccugaucg ggacccuacu cauguggcua ggccucaaua     2040

ccaaaaacgg auccaucagc cuaaugugcc uggcccuagg cggaguccug aucuuccucu     2100

caacagcagu cagcgcagac ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  7237
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7237
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gcgcggacac gucagucggc auagucgguc uacuacuuac aaccgccaug gcagccgccg      120

uuacacgccg gggauccgcc uacuacaugu accucgaccg caacgacgcc ggcgaagcaa      180

ucucguuccc aaccacacua ggcaugaaca aaugcuacau ccaaauuaug gaccuaggac      240

acaugugcga cgccaccaug agcuacgagu gcccaaugcu agacgaagga guggagccag      300

acgacgugga cugcuggugc aacacuacau cgaccugggu cgucuacggg accugccacc      360

acaagaaagg agaagcacga aggucaagac gagcuguaac acuaccaagc cacuccacaa      420

ggaaauuaca gacccgaagc caaacauggc ucgaauccag ggaauacacc aaacaucuca      480

uucgcguaga aaacuggaua uuccgcaacc caggcuucgc acuagcggcg gccgcgauag      540

cuuggcuccu cgggagcucc accucgcaaa aaguaaucua ccuagucaug auccuacuca      600

uagcucccgc guacagcauc agaugcaucg gcguaagcaa ccgcgacuuc gucgaaggca      660

ugagcggcgg caccugggua gacauaguac ucgaacacgg gggcuguguc accguaaugg      720

cacaagacaa accaacggug gauaucgagc uagucacaac gaccgucucc aacauggccg      780

aaguaagaag cuauugcuac gaggcaucca uaagcgacau ggcaagcgac ucgcgcugcc      840

caacgcaagg ugaagcauac cuagacaaac aaagcgacac acaauacgua ugcaaacgca      900

cccucgucga cagagggugg ggcaauggau gcggacucuu cggcaaaggc ucccucguca      960

caugcgcaaa auucgccugc uccaaaaaga ugacaggcaa aucaauccaa cccgaaaacc     1020

uagaguaccg aauaaugcuc agcguccacg gaagccaaca cagcggaaug auagucaacg     1080

acacaggaca cgaaaccgac gaaaaccgcg caaaagucga aaucacacca aacaguccaa     1140

gagcggaagc aacacuaggc ggauucggau cacuaggacu agauugcgag ccacgcacag     1200

gacuagacuu cagcgaccua uacuaccuga cgaugaacaa caaacacugg cucguacaca     1260

aagagugguu ccacgacauc ccccucccau ggcacgccgg cgccgacacc ggcaccccgc     1320

auuggaacaa caaagaagcc cuaguagagu ucaaagacgc acacgcaaag cgccaaaccg     1380

ucgucguacu aggaagccaa gaaggagcgg uccacacagc ccuagccggc gcccuagaag     1440

cugaaaugga cggagcaaaa ggaagacuaa gcagcgguca ccucaaaugc agacucaaaa     1500

uggacaaacu acgccucaaa ggcguaucgu acucccucug caccgccgcc uucacauuca     1560

ccaaaauacc agcagagaca cuacacggaa ccgugacagu ggaaguccaa uacgccggca     1620

ccgauggacc augcaaagua cccgcacaaa uggcaguaga caugcaaacc cugacacccg     1680

ucggcagacu aaucacagcc aacccaguca uaaccgaaag caccgagaac agcaagauga     1740

ugcucgagcu cgacccgcca uucggggaca gcuacaucgu cauaggagug ggagaaaaga     1800

aaaucaccca ccacuggcac cgaagcggcu caacaauagg aaaagcguuc gaagcgaccg     1860

uccgaggagc caaacgcaug gcaguacucg gugacacagc cugggacuuc ggcucaguag     1920

gcggcgcccu aaacucucua ggaaaaggca uacaccaaau cuuuggcgca gccuucaaau     1980

cacucuucgg cggcaugagc ugguucuccc agauccuaau aggcacacua cuaauguggc     2040

ucggacucaa caccaaaaau ggcucgauca gccugaugug ccucgcacua ggcggcguac     2100

ugaucuuccu auccacagcg guaagcgccg acugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  7238
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7238
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccuaucguc acggaaaaga cggagccacg acgugcuaac agugcaauuc cucauacucg      120

gcaugcuacu aaugaccgga ggcgucaccc gccgaggcuc agccuacuac auguaccuag      180

acaggaacga cgccggggaa gccauaaguu uccccacgac acugggcaug aacaaaugcu      240

auauacaaau cauggaucua ggccacaugu gcgacgccac caugagcuac gaaugcccga      300

ugcuagacga aggagucgag ccagacgacg uggacuguug gugcaacaca acauccacau      360

ggguagucua cggaacaugc caccauaaga aaggagaagc ccgacgcuca aggagagccg      420

ugacccuccc aagccacucg accagaaaac uccaaacaag aagccaaacg uggcucgaau      480

cgagggaaua cacgaagcac cuaauaagag ucgaaaacug gauauuccga aacccaggau      540

ucgcacuggc cgcggccgcc aucgccuggc uacucggaag cagcacgagc caaaaaguaa      600

uuuaucuugu aaugaucuug cugauagccc cggccuacag cauccgaugc aucggcguuu      660

cgaauagaga cuucguagaa ggcaugagcg gcgguaccug gguagauauc guccucgaac      720

acgggggaug cgugaccgua auggcccagg acaaaccgac cgucgacaua gagcuaguaa      780

ccacaacagu cagcaacaug gcagaaguca ggaguuacug cuaugaagca ucaauaucag      840

acauggcaag cgacagcaga ugcccuacac aaggggaggc auaccucgac aaacaaagug      900

acacccaaua cgucuguaaa agaacccuag ucgaccgcgg augggggaau ggcugcggac      960

uauucggaaa agggagccua gugacaugcg ccaaauucgc augcucaaag aaaaugacag     1020

gaaaaagcau ccaaccagaa aaccucgagu acagaauaau gcucagcguc cacgggucac     1080

agcacagcgg caugauagua aacgacaccg gacacgaaac cgacgaaaac cgagccaaag     1140

uagaaauaac cccuaauagc cccagagcug aggcaacccu aggaggcuuc ggcagcuugg     1200

gacucgacug cgaaccccga acgggacucg acuucuccga ccuauacuac cucaccauga     1260

acaacaaaca cuggcucgug cacaaagaau gguuccacga caucccccug cccuggcacg     1320

ccggcgccga cacaggaacg ccucacugga acaacaagga agcccuugua gaguucaaag     1380

acgcgcacgc aaaacgccaa acgguggucg ugcucggaag ucaagaagga gccgugcaca     1440

cggcacuagc cggcgcgcuc gaagccgaaa uggacggagc aaaggggcga cugagcagcg     1500

gacaccugaa augccggcug aaaauggaca aacugagacu aaaaggaguc agcuacagcc     1560

uaugcaccgc cgcauucaca uucacaaaaa uaccggccga aacacuacac gguacaguca     1620

cgguagaagu ccaauacgcg gggacagacg gacccugcaa agucccagcg caaauggcgg     1680

uagauaugca gacacucaca cccgugggua gauuaauaac agcaaacccc gucaucacag     1740

aaagcacgga aaacagcaaa augaugcuag aacuagaccc acccuucgga gauucauaca     1800

uagucauagg cguaggcgaa aagaaaauaa cccaccacug gcaccgcagc ggcagcacaa     1860

ucggaaaagc auucgaagca acggugaggg gagccaaacg aauggcgguc cucggagaua     1920

cagccuggga cuucggaagu gucgggggug cacugaacag ccuaggcaaa ggcauacacc     1980

aaaucuucgg agcagcguuc aaaagccucu ucgggggaau gagcugguuc ucccaaauau     2040

ugauaggaac ucuacucaug uggcucgggc uaaacacuaa aaauggcagc aucagccuca     2100

ugugccuggc ccucggaggc guccucauau uccuaagcac agccgucagc gccgacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  7239
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7239
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgag       60

gccuuagcuc ccgcaaacga cgcagccacg acguacuaac cguccaauuc cuaauacuag      120

gcaugcuccu caugacaggc gccguaacaa gacgggguuc cgcauacuac auguaccucg      180

acaggaacga cgcgggagaa gccauuuccu ucccaaccac acucggcaug aacaaaugcu      240

acauacaaau aauggaucuc ggacacaugu gcgacgccac caugucauac gaaugcccaa      300

ugcuagacga aggaguggaa cccgacgacg uagacugcug gugcaacacc acauccaccu      360

ggguagucua cggaacgugc caccacaaga aaggagaagc acggcgaagc cgacgagccg      420

uaacccuccc cucccacagc acucgcaaac uccaaacacg aucacaaaca uggcuagaau      480

cccgcgaaua caccaaacac cuaauacgcg ucgaaaacug gaucuuucga aacccaggcu      540

ucgcccucgc agcagccgcg auagcauggc uucuuggaag cagcacaagu caaaaaguaa      600

ucuacuuagu aaugauccug cuuauagcgc cugcauauuc gauacggugc aucgggguaa      660

gcaaccgcga cuucgucgaa ggaaugucag gaggcaccug gguagacauu guacucgaac      720

acggaggaug cguaaccgua auggcccaag acaaaccuac agucgacaua gagcucguaa      780

cgaccacagu aagcaacaug gcagaggucc gaucauacug cuacgaagcc ucaauaagug      840

acauggccuc agacucacgc ugcccaaccc aaggcgaggc cuaccuagac aagcaauccg      900

acacacagua cgucugcaaa cgcacacucg ucgacagagg guggggcaac gguugcggcc      960

uuuucggcaa aggaucacuc guaacaugcg caaaauucgc cugcuccaag aaaaugacug     1020

gaaaaagcau acaaccagaa aaccuagagu acagaauaau gcuauccgua cacgggagcc     1080

agcacucagg aaugaucguc aacgacacgg gacacgaaac cgacgaaaac cgcgcaaaag     1140

uagaaauaac acccaacuca ccccgagcag aagcgacccu aggaggauuc ggaucccucg     1200

gacuagacug ugaacccaga acaggccucg acuucagcga ccucuacuac cucaccauga     1260

acaacaaaca cuggcuuguc cacaaagagu gguuccacga caucccccua cccuggcacg     1320

ccggagcaga caccggcacc ccacacugga acaacaaaga agcccucguc gaguucaaag     1380

acgcgcacgc aaaacgacaa accgucgucg uucuuggauc ccaggaagga gccguccaua     1440

cagcacuagc aggcgcccug gaagccgaaa uggacggcgc caaggggcgg cuaucaucag     1500

gccaccuaaa augucgccua aagauggaca aacuccgacu aaaagggguc ucauacucuc     1560

uaugcaccgc ggcauucacc uucaccaaga uccccgcaga aacccuacac ggaaccguca     1620

caguagaagu acaauacgcc ggcacagacg gccccugcaa ggucccagcc caaauggccg     1680

uagacaugca aacacugacc ccaguaggac gacuaauaac cgcgaacccc guaauaaccg     1740

aaucgaccga aaacucaaaa augaugcucg aacuagaccc accguuuggc gacagcuaca     1800

ucgucaucgg cgucggagag aaaaagauaa cccaccacug gcacaggucc ggaagcacca     1860

ucgguaaggc uuucgaagca accguccgcg gagccaaacg cauggccguc cuaggagaca     1920

cagcauggga uuucggcagc gucggcggag cccugaacag ccucggaaaa ggaauacacc     1980

aaauauucgg agccgcguuc aaaagccuau ucgggggcau guccugguuc ucacaaaucc     2040

uaaucggcac acuacugaug uggcucggcc ucaacaccaa aaacgggagc auuagccuca     2100

ugugucuagc ucuaggcggc guacuaaucu uccuauccac agcagugagc gcggacugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  7240
<211>  2494
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7240
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucauaaacgc cagaaaagaa aagaaacgaa ggggagccga cacaucagua ggcaucgucg      120

gacuacucuu gaccaccgca auggccgcgg aagucaccag aaggggcuca gcauacuaca      180

uguaccuaga ccguaacgac gccggagaag caaucuccuu ccccaccacc cuuggaauga      240

acaaaugcua cauacaaaua auggaucuag gccauaugug cgacgccacc auguccuacg      300

aaugccccau gcucgacgaa ggaguagagc ccgacgacgu ggacuguugg uguaauacca      360

ccagcacaug ggucgucuau gggaccugcc accacaagaa aggcgaagca cgaagaaguc      420

gacgagcagu aacacuaccg agccacagca cccgaaaacu acaaacaaga agucaaacau      480

ggcuagaaag ccgagaauac acgaaacacc uaauaagagu agagaacugg auauucagaa      540

acccaggguu cgcccucgcc gccgcggcca ucgcauggcu gcuaggcucc agcacaucgc      600

aaaaaguaau auaccuaguc augauucuac uaaucgcacc cgcauacagc auccgcugca      660

uaggcgucuc gaaccgcgac uucgucgagg gcaugucagg aggcacaugg guggacaucg      720

ucuuagaaca cgguggaugc gucaccguca uggcccaaga caaacccacc guagacaucg      780

agcugguaac caccaccgua uccaacaugg ccgaagugcg guccuacugc uacgaagccu      840

caauaagcga cauggccucc gacagcagau gcccaaccca aggggaggcg uaucucgaca      900

aacaaagcga cacccaauac gucugcaaac gaacacucgu agaccgcggg uggggaaacg      960

gcugcggacu cuucggaaaa ggcagccucg uaaccugugc caaauucgca ugcucgaaga     1020

aaaugaccgg aaaauccaua caaccagaga acuuagaaua ccgcaucaug cuaagcguac     1080

auggcagcca acacagcggc augauaguaa acgacacagg ccaugaaaca gacgaaaacc     1140

gagcaaaagu cgaaaucacc cccaacagcc cgagagcaga agccacacua ggaggauucg     1200

gcagccuagg ccuggacugc gaaccaagga caggccucga cuucagcgac cucuacuacc     1260

ucaccaugaa caacaagcac uggcuaguac acaaagaaug guuccacgac auaccacucc     1320

ccuggcacgc aggcgccgac acagggacac cccauuggaa uaacaaagaa gcacugguag     1380

aguucaaaga cgcccacgcc aagagacaaa ccguaguagu acuaggcucc caggaagggg     1440

caguccacac agcacuagcg ggagcacugg aagcagaaau ggacggcgcc aaagggcgac     1500

uaagcagcgg ccaccugaaa ugcagacuua aaauggauaa acuacgacua aagggcguca     1560

gcuacagccu cugcaccgcu gccuucacau ucacgaaaau ccccgccgaa accuuacacg     1620

gcacggucac agucgaagua caauacgccg gaaccgacgg uccaugcaaa guacccgcac     1680

aaauggccgu cgacaugcag acacuaacac ccgucggacg gcuaauaaca gccaaccccg     1740

uuaucacaga aucgaccgaa aauucaaaaa ugaugcucga acuagaccca ccguuuggag     1800

acuccuacau cguaaucggc gucggagaaa agaaaaucac acaccacugg caccgcagcg     1860

gcuccacgau agggaaagca uucgaggcca ccguccgagg agcaaaaaga auggccgucc     1920

ucggcgacac agcaugggac uucggcagcg ucggaggcgc gcucaacagu cucggaaaag     1980

gcauccacca aauauucggc gcagcauuca aaagccuuuu cggcggcaug agcugguuuu     2040

cacaaauccu aauaggaacc cuccucaugu ggcucggccu aaacaccaaa aacgggucaa     2100

uaagccuaau gugccuggcc cucggcgggg uccuaauauu ccucuccacg gcagucagcg     2160

caugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     2220

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     2280

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2340

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2460

ccccccaaag gcucuuuuca gagccaccag aauu                                 2494


<210>  7241
<211>  2488
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7241
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

acgcccgaaa agagaaaaag cgccgaggag ccgacacauc cguagguaua gucggccucu      120

uauuaacaac agcaauggcu gccgaaguaa caagacgcgg cagcgcauau uacauguacc      180

uugaccgaaa cgaugcaggg gaagcaauau cauuuccaac aacacuuggc augaacaaau      240

gcuauaucca aaucauggac uugggacaca ugugcgacgc cacaauguca uacgaaugcc      300

cgaugcuaga cgaaggggug gaaccggacg acguugacug cuggugcaac acaacuucaa      360

ccuggguagu cuacggaacc ugccaccaca agaaaggcga agcaagaaga uccagacggg      420

ccgucacacu ccccagccac agcacuagaa aacuacaaac ccgaagccaa acauggcucg      480

aaagccgcga auacacaaag caucugaucc gaguggaaaa cuggaucuuc cgaaaccccg      540

gcuucgcccu cgccgccgca gccauagcau ggcuccuggg auccuccacg ucacaaaaag      600

ugauauaccu aguaaugauc cugcucauag ccccggccua cuccauacgc ugcauaggcg      660

uaagcaaccg agacuucgua gagggaaugu cagggggcac cugggucgac aucguccuag      720

aacacggggg cugcgucaca gucauggcgc aagacaagcc cacagucgac aucgagcucg      780

ucacaacaac cguaucaaac auggcagagg uaagguccua cugcuacgaa gcgucaaucu      840

cagacauggc cagcgacucc cguugcccca cacaaggcga ggccuaccua gacaaacaau      900

ccgacacaca auacguaugc aaacgaacac ucguagaccg gggcugggga aacggcugcg      960

gacucuucgg caaaggcagc cucgucacau gcgcgaaauu cgccugcuca aagaaaauga     1020

ccggaaaaag cauccaaccc gagaaccuag aauaccgaau aaugcuaagc guacacgggu     1080

cccaacacuc cggaaugauc gucaacgaca ccggacacga aaccgacgaa aacagagcua     1140

aaguagaaau cacgccaaac ucgccacgcg cagaagccac gcucggagga uucggaucac     1200

uagggcuaga cugcgagcca cgcaccgggc uagacuucuc cgaccuauac uaccuaacca     1260

ugaacaacaa acacuggcuu guucacaaag aaugguucca ugacaucccc cuccccuggc     1320

acgcaggagc cgacacagga accccacacu ggaacaacaa agaagcccua guagaguuca     1380

aagacgcaca ugcaaaacgc cagaccgucg ucguacuagg aucccaagaa ggagcugucc     1440

auaccgcacu cgccggcgcc cucgaagccg aaauggacgg agccaaaggg cgccuaagcu     1500

ccggacaccu caaaugccga cuaaaaaugg acaaacuaag acugaaaggc gucagcuaca     1560

gccuaugcac agccgcauuc acauucacca aaauuccagc agaaacgcuc cacggaacag     1620

ucaccguaga aguacaauac gccggcacag acggccccug caaagugcca gcccaaaugg     1680

cgguagacau gcaaacacuc acaccagucg gccgccuaau aacggcuaac ccaguaauca     1740

ccgaaagcac cgaaaauagc aaaaugaugc uugaacucga cccgcccuuc ggagacagcu     1800

acaucgucau cggcgucggg gaaaagaaaa uaacacacca cuggcaccga agcgggucca     1860

caaucggcaa agccuucgaa gcaaccguaa ggggcgcaaa acgcauggcc guacucggag     1920

acacggccug ggacuucggu uccgucggag gcgcacuaaa cagccugggc aaaggcaucc     1980

aucaaaucuu cggagcagca uucaaaucac uuuuuggggg aaugagcugg uucucccaaa     2040

uccugaucgg caccuuacua auguggcugg gccuuaacac caaaaauggc agcaucucgc     2100

ucaugugccu cgcacucgga ggaguccuaa ucuuccugag cacagccgua uccgccugag     2160

gacuagugca ucacauuuaa aagcaucuca gccuaccaug agaauaagag aaagaaaaug     2220

aagaucaaua gcuuauucau cucuuuuucu uuuucguugg uguaaagcca acacccuguc     2280

uaaaaaacau aaauuucuuu aaucauuuug ccucuuuucu cugugcuuca auuaauaaaa     2340

aauggaaaga accuagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2460

aaaggcucuu uucagagcca ccagaauu                                        2488


<210>  7242
<211>  2491
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7242
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugauaa       60

ucaacgcaag aaaggaaaag aaacgccgcg gugccgacac aucaguaggc auagucggcc      120

uccuccucac gacagcuaug gccgcagaag uaacacgacg cggaagcgcg uacuacaugu      180

accucgaccg caacgacgcc ggagaagcga ucuccuuccc gaccacacuc ggcaugaaca      240

aaugcuacau ccaaaucaug gaucucggac acaugugcga cgccaccaug uccuacgagu      300

gcccaaugcu agacgaagga gucgaacccg acgacguaga uugcuggugc aacacgaccu      360

ccacaugggu cguuuacgga accugucacc acaagaaggg cgaagcccga cgaagccgca      420

gagcagucac ccuacccagc cacuccacuc gcaaacucca gacacgaagc caaacauggc      480

ucgagagccg agaguacaca aaacaccuca uaaggguaga aaacuggauc uuccgaaauc      540

caggcuucgc ccuagccgca gccgccaucg cguggcuucu aggcucaagc acgucacaaa      600

aggucaucua ccucgucaug auccuacuaa ucgcuccagc cuauucaauc cggugcauag      660

guguaagcaa ccgagacuuc guagaaggca ugucaggagg aacaugggua gacaucgucc      720

uggaacaugg cggaugcgua accgucaugg cccaagacaa acccacagua gacaucgaac      780

ucgugaccac caccguaagc aacauggcag aaguccgaag cuacugcuac gaggcuucaa      840

ucagcgacau ggcaucagac agcagaugcc ccacacaagg cgaagccuac cuagacaagc      900

agucagacac ccaauaugua ugcaaacgca cccuagucga uaggggaugg ggaaacggau      960

gcggacucuu cgguaaaggc agucucguga ccugcgcgaa auucgcgugc uccaagaaaa     1020

ugacgggcaa aagcauucaa cccgaaaacc uagaauacag aaucaugcua ucaguccacg     1080

gcucccaaca cuccggcaug auugucaacg acaccggcca cgaaaccgac gaaaaccgag     1140

caaaagucga aaucacaccc aacagcccgc gcgccgaagc cacacucggc ggcuucggca     1200

gccugggccu cgacugugaa ccccgcacag gacuagacuu cagcgaccuc uacuaccuga     1260

caaugaacaa caaacacugg cucguccaca aagaaugguu ccacgacauu ccccuccccu     1320

ggcacgccgg cgcagauacc ggaaccccac acuggaacaa uaaagaagca cucguagagu     1380

ucaaggacgc gcacgcaaaa cggcaaacag ucgugguccu gggcucgcaa gaaggcgcag     1440

uccacacagc ccucgccggc gcccuagaag ccgaaaugga cggagcaaaa gguagacuca     1500

gcucaggaca ccuaaaaugc agacucaaga uggacaaauu acgccucaaa ggcguaucuu     1560

acucccuaug caccgccgca uucaccuuua ccaaaauccc ggcagaaacg cuccauggaa     1620

cagucaccgu cgagguccaa uacgccggaa ccgauggacc uugcaaaguc cccgcccaaa     1680

uggcgguuga caugcagacc cugacccccg ucggccgacu caucaccgcc aacccaguca     1740

ucacugaaag cacagaaaac uccaaaauga ugcucgaacu cgacccaccc uucggcgaca     1800

guuacauugu caucggcguc ggggaaaaga agaucacaca ccauuggcac cgguccggaa     1860

gcacgauugg aaaggccuuc gaagcaaccg ucagaggagc caaaagaaug gcgguacucg     1920

gcgacaccgc augggacuuu ggcuccgucg gcggagcacu uaacucacuc ggaaaaggca     1980

uacaccaaau auucggugcu gccuucaaau cacucuucgg cggcaugagc ugguucucac     2040

agauacuaau cggaacacua cuaauguggc uaggccucaa caccaaaaac ggaagcauau     2100

cacuuaugug ccuagcccuc ggcggaguac uaaucuucuu guccacagca gucuccgccu     2160

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2220

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2280

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2340

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2460

cccaaaggcu cuuuucagag ccaccagaau u                                    2491


<210>  7243
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7243
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaacg       60

ccagaaaaga aaagaaacga agaggcgccg acaccagcgu uggaaucguc ggacuacuac      120

uaaccaccgc gauggcagcc gaagucacga gaaggggaag cgccuacuac auguaccuag      180

acagaaacga cgcaggcgag gcaauaaguu uccccacaac gcuaggaaug aauaaauguu      240

acauacaaau aauggaucuc ggacacaugu gcgacgcaac aaugagcuac gaaugcccaa      300

ugcuagacga aggagucgaa ccagacgaug uggacugcug gugcaacaca acuagcacau      360

gggucgucua cggcacaugc caccacaaga aaggagaagc acgaaggagu cgcagagccg      420

ucacgcuacc cagccacuca acgagaaaac uccagaccag gagccaaacg uggcuagaga      480

gcagagaaua cacaaaacac cuaauaagag ucgaaaacug gaucuuccga aacccaggcu      540

ucgcccucgc cgccgcagcc auagccuggc uccuagguuc uaguaccagc caaaaaguaa      600

uauaccuagu gaugauccua cugauagcac ccgcauacuc cauccguugc auaggaguca      660

gcaaccgcga cuucguagaa ggaaugagcg gaggaaccug ggucgauauc guacuagaac      720

acggcggcug cgucacaguc auggcccaag acaaaccaac cgucgacaua gaacucguga      780

caacaacagu uagcaacaug gcggaagugc gaagcuacug cuaugaagcc uccauaucag      840

acauggcauc ggacagccga uguccaacac aaggagaagc cuaccuagac aagcaauccg      900

acacacaaua cguaugcaaa agaacccuag ucgaucgagg cuggggcaac gggugcggcc      960

uuuucggaaa aggaagccug gucacaugcg ccaaauucgc gugcagcaag aaaaugacag     1020

gcaaaucaau ccaaccagaa aaccucgaau aucgaaucau gcucucggua cacggcagcc     1080

aacacuccgg aaugaucgua aacgacacag gucacgagac agacgaaaac agagcaaaag     1140

ucgaaaucac gcccaacagc ccaagagccg aagcaacacu cggcggguuc gggagccuag     1200

gacuagacug cgaaccccga acaggccucg acuucucgga ccucuacuau cuuaccauga     1260

acaacaaaca cuggcuaguc cacaaagaau gguuccacga caucccccuc cccuggcacg     1320

caggggcaga caccggaacg ccgcauugga acaauaaaga ggcucuaguc gaguucaagg     1380

acgcacacgc gaaaagacaa accgucgucg uauuaggcuc acaagagggc gccguccaca     1440

cagcgcucgc aggggcacua gaagccgaga uggacggcgc gaaaggaagg cuaucaucag     1500

gucaccucaa augccggcuu aagauggaca aacugagacu aaaaggggua uccuauucac     1560

ucugcacagc agcauucaca uucacaaaaa uccccgcaga aacacugcac ggaacaguaa     1620

ccguagaagu ccaauacgca ggcaccgacg gcccaugcaa aguaccugcc caaauggccg     1680

uagacaugca gacccuaacg cccgucggcc ggcucaucac ggccaacccg guaauaacug     1740

aaucaacaga aaacucuaaa augaugcugg aacuggaccc acccuucggc gacaguuaca     1800

uagucaucgg ugucggugaa aagaaaauca cccaccacug gcacagaagc ggaagcacua     1860

uaggcaaagc guucgaagcc acaguaagag gcgcaaaaag gauggcagua cuaggagaca     1920

ccgccuggga cuucggcuca guaggaggag cacuaaacuc gcucggcaag ggaauccacc     1980

aaauauucgg agccgcauuc aagagccuau ucggcggaau gagcugguuc ucacaaaucc     2040

uaaucggaac gcugcuaaug uggcucggcc uaaacacaaa gaacggcagc aucagccuaa     2100

ugugccucgc ccucgggggc guccuuauau ucuugagcac agcaguaagc gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  7244
<211>  2482
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7244
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ggaaagagaa aaagagaaga ggcgcggaca caucaguagg aauagucgga cuacugcuaa      120

caaccgccau ggcagcagaa guaacccgaa gaggcucagc cuacuacaug uaccucgacc      180

gaaacgacgc cggagaagcc auaucauucc ccaccacccu cggaaugaac aaaugcuaca      240

uccagaucau ggaccucggc cacaugugcg augcaaccau gagcuacgaa ugcccaaugc      300

ucgacgaagg cguagaacca gacgacguag acugcuggug caacacaacc aguacguggg      360

uagucuaugg cacaugccac cacaagaaag gcgaagcacg cagaagccgc cgcgcaguca      420

cucucccauc ucacagcacc agaaaacucc agacaaggag ccagaccugg cuggaauccc      480

gagaauacac caaacaccua auaagggucg aaaacuggau uuuccgcaac ccaggcuucg      540

cacucgcagc cgcagccaua gccuggcuac ugggcagcag cacaagccaa aaagugaucu      600

aucucgucau gauacuccug auugccccag cuuacuccau cagaugcauc ggcguaagca      660

accgagacuu cgucgagggg augucgggag gaaccugggu ugacaucgua cucgaacacg      720

gcggaugcgu aaccguaaug gcucaagaca aacccaccgu ugacauagaa cuggugacaa      780

ccacaguauc caacauggca gaggugagau cauacugcua cgaggcuuca aucucugaca      840

uggccucgga cagccgcugc cccacacagg gggaagcgua ccucgacaaa caaucagaca      900

cacaguacgu cugcaaacga acccucguag accgcggaug gggcaacggg ugcgggcuau      960

ucggcaaagg aucacucguc acaugcgcaa aauucgcuug cucgaagaaa augacaggca     1020

aaagcauaca accagagaac cucgaauaca gaauaaugcu gucaguacac ggcucacaac     1080

acagcggaau gauugucaac gacacaggac acgagaccga cgaaaacagg gccaaaguag     1140

aaauaacccc caacagcccc cgagccgagg caacacuugg aggauucggc ucccuaggau     1200

uagacugcga accgcgcacc ggacuggacu uuagcgaccu auacuaccuc acaaugaaca     1260

acaaacacug gcuaguccac aaagaguggu uccacgacau accccuccca uggcaugccg     1320

gagcagacac aggaacccca cacuggaaca acaaagaagc gcugguagag uucaaagacg     1380

cccacgcaaa acgccaaaca gucguggucc ucggcagcca agaaggcgcc guccacaccg     1440

cacucgccgg cgcacuagaa gcagaaaugg acggcgcgaa aggccggcuc agcuccgggc     1500

accuaaaaug uagacuaaaa auggauaagc uacgccuaaa agggguauca uacagccuau     1560

gcaccgccgc cuucacauuc acaaaaauac cggcagaaac acugcacgga acaguaacag     1620

ucgaaguaca auacgcagga accgauggcc caugcaaagu uccagcccaa auggcagucg     1680

acaugcaaac acugaccccg gucggaagac uuaucacagc aaaccccguc aucacagaau     1740

caaccgaaaa cagcaaaaug augcucgaac uagacccacc auucggcgac aguuacauag     1800

ugauaggcgu aggagagaag aaaaucaccc aucacuggca cagauccggg agcacuauag     1860

ggaaagccuu cgaggccaca guaaggggag ccaaacgcau ggccguccuc ggugacacag     1920

caugggacuu cggcucagua ggcggcgccc ucaacucccu cggaaaagga auccaccaaa     1980

uauucggggc cgcauucaag agccuguucg ggggcaugag cugguucucc caaauccuaa     2040

uaggcacccu ccucaugugg cuaggccuca auaccaaaaa cggaaguauc ucacucaugu     2100

gccuagcacu aggcggcguc cucauauucc ucagcacagc cguuagcgcg ugaggacuag     2160

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     2220

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     2280

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2340

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2460

ucuuuucaga gccaccagaa uu                                              2482


<210>  7245
<211>  2479
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7245
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

aagagaagaa aagacgcgga gcagacacca gcgucggaau cgucggccuu cuccucacua      120

cagccauggc cgccgaaguc acgagacgcg gauccgcaua cuacauguau cuagaccgaa      180

acgacgccgg cgaggcaauc ucauucccca ccacccucgg caugaauaaa ugcuauaucc      240

agauaaugga ccucggccac augugcgacg caacaaugag cuacgaaugc cccaugcucg      300

acgagggagu agaacccgau gacguugacu gcuggugcaa cacaaccucg accugggucg      360

uguacggcac augccaccac aagaaaggag aagcgagacg cucccgucga gcaguaaccc      420

uuccauccca cagcacccgg aaacuccaaa cacgcuccca aaccuggcuc gaaucccgcg      480

aauauacaaa acaucuaauc cgaguagaaa acuggaucuu ccgcaacccg ggauucgccc      540

uggcagcggc cgccaucgcc uggcuacucg guagcuccac cagccaaaaa guaaucuacc      600

ucguuaugau acuacuaauc gccccagcau acucaauacg cugcaucggc guaagcaacc      660

gagacuucgu cgaaggaaug ucaggcggca ccugggucga caucguccua gaacacggcg      720

gaugcgucac cgucauggcg caagacaaac caaccguaga cauagagcua guaacaacca      780

ccguaucaaa cauggccgaa guccgaagcu acugcuacga agcaucgaua uccgacaugg      840

ccuccgacuc acgcugcccc acccaaggag aagccuaccu agacaaacag ucggauacgc      900

aauacgucug caaacgaacc cuagucgacc gaggcugggg aaacggcugc ggccucuucg      960

gaaaaggguc ccuaguaacc ugcgcaaaau ucgcaugcag caagaaaaug acaggcaagu     1020

ccauccagcc cgaaaaccuc gaauaucgaa uaaugcucuc cguccacggc ucacaacaca     1080

gcggaaugau cgucaacgac accggacacg aaaccgacga aaaccgagca aaagucgaga     1140

uaaccccaaa cucaccucgc gcugaggcaa cacucggagg cuucggcucc cucggacucg     1200

acugcgaacc acgaaccggg cuagacuuca gcgaucuaua uuaccucacc augaacaaca     1260

aacacuggcu aguccacaaa gaaugguucc acgacauacc acuccccugg cacgccggcg     1320

cagacaccgg cacgccccac uggaacaaca aagaagcccu ggucgaguuc aaagacgccc     1380

acgccaaacg ucaaaccguc gugguacucg ggucccaaga aggagcaguc cacacagcac     1440

uugcgggcgc ccuggaagcc gaaauggacg gcgccaaagg acgucucucc agcggacacc     1500

uaaagugccg ccugaaaaug gacaaacuac gacuaaaggg agucucauac agcuuaugca     1560

ccgccgccuu cacauuuacc aaaauacccg cagaaacgcu acacggcacc guaaccgucg     1620

aaguacaaua cgcgggaaca gacggacccu gcaaaguacc ugcucaaaug gccguagaca     1680

ugcaaacacu cacaccaguc ggaagacuaa ucacagcaaa ccccguaauc accgaaagca     1740

ccgaaaauuc caaaaugaug cucgaacuag acccaccguu uggagacucu uacaucguaa     1800

uaggaguggg cgaaaagaaa aucacacauc acuggcaccg aucgggaucg accauaggua     1860

aagccuucga agcaacagug cgaggcgcca aacgaauggc uguacuugga gacaccgcau     1920

gggauuuugg cuccguugga ggagcccuua acagccucgg caaaggcaua caccagauuu     1980

ucggcgcagc cuucaaaucc cucuucggcg gcauguccug guucucccaa auccucaucg     2040

gaacccuacu cauguggcua ggccucaaca cgaaaaacgg aucaauaucc cucaugugcc     2100

uagcacuggg cgguguacua auauuccucu ccaccgccgu cucagcguga ggacuagugc     2160

aucacauuua aaagcaucuc agccuaccau gagaauaaga gaaagaaaau gaagaucaau     2220

agcuuauuca ucucuuuuuc uuuuucguug guguaaagcc aacacccugu cuaaaaaaca     2280

uaaauuucuu uaaucauuuu gccucuuuuc ucugugcuuc aauuaauaaa aaauggaaag     2340

aaccuagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2460

uuucagagcc accagaauu                                                  2479


<210>  7246
<211>  2476
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7246
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaag       60

agaaaaagag gagaggcgcg gacacguccg ucgguauagu aggccuacuu cuaacaaccg      120

ccauggccgc agaaguaacc cgucgaggaa gcgccuacua cauguaccuc gaccgaaacg      180

acgccggcga agcgaucagc uuccccacca cccucggcau gaacaaaugu uacauccaga      240

uaauggaccu cggccacaug ugcgacgcca cgaugucaua cgaaugcccc augcuugacg      300

aaggcguuga accagacgac gucgacugcu ggugcaacac uacaagcacc uggguggucu      360

acggcacuug ccaccacaaa aagggagaag cacgccgcag ccgucgagcc guaacccuuc      420

ccucacacag cacccgaaag cuccaaaccc gcucccaaac cuggcuagaa ucucgagaau      480

acaccaaaca ccucauuaga gucgaaaacu ggauauuccg caauccaggc uucgcccuag      540

cagcagccgc aaucgccugg cuucucggau ccucaacauc acagaaaguc auauaccucg      600

uuaugauacu ccuaaucgcc ccagccuaca gcaucagaug caucggcguc agcaaccgag      660

acuucguaga aggcaugagc ggaggaacau ggguagacau cguccucgaa cacggaggau      720

gcgucaccgu aauggcccaa gacaaaccca caguugacau ugagcuaguc acaaccaccg      780

ugagcaacau ggccgaagug cgcagcuacu gcuacgaagc cuccauauca gacauggcau      840

cagacagccg cugcccuaca cagggagaag cauaccugga caaacagucc gacacacaau      900

acgucugcaa acgaacccuc guagaccgag gcuggggaaa cggaugcgga cuauucggca      960

aaggcucccu cgucacaugc gcaaaguucg ccugcagcaa gaaaaugacc ggcaaaucua     1020

uccagcccga gaaccucgaa uacagaauaa ugcucuccgu acacggaucc caacacagcg     1080

gcaugaucgu caacgacaca ggacacgaaa cugacgaaaa ucgcgcgaaa gucgaaauca     1140

cacccaacuc cccucgagcg gaagcuaccc ugggcggcuu cggcagccua ggccucgauu     1200

gcgaaccacg aacaggcuua gacuucuccg accucuacua ccuaacgaug aacaauaaac     1260

acuggcuagu ccacaaagaa ugguuccacg acauaccguu accguggcac gccggggccg     1320

auaccggaac accacauugg aacaacaaag aagcacuggu agaguucaaa gacgcccacg     1380

caaaacgcca aaccgucguc guacucgggu cccaagaagg cgcaguccac acagcccucg     1440

caggagcccu cgaagccgaa auggacggcg caaaaggccg ccucagcagu ggucaccuaa     1500

aaugcagacu aaaaauggac aaacuccgac uaaaaggcgu cucguacucc cuguguaccg     1560

cagcuuucac uuucaccaag auccccgcag aaacccucca cggcaccguc accguagaag     1620

ugcaauacgc agggaccgau ggccccugua aaguaccggc ccaaauggca gucgacaugc     1680

agacccugac acccgugggc cggcucauca cagcaaaccc cguaauaaca gaauccaccg     1740

aaaacuccaa gaugaugcua gagcuagauc ccccauucgg cgacagcuac auagucaucg     1800

gaguaggaga aaagaaaauc acacaccacu ggcaccgguc cggcuccacc auaggcaaag     1860

cauuugaagc uacaguccgc ggcgccaaac gcauggcagu acuuggcgac accgccuggg     1920

acuucggcuc cguaggaggg gcgcucaaca gccuagggaa aggaauccac caaaucuuug     1980

gagccgcauu caaaucccua uucggcggca uguccugguu cucucaaauc cugaucggca     2040

cccuacucau guggcuaggc cucaacacca aaaacggcuc caucucccua augugccugg     2100

cacucggagg aguacucauc uuccuaucca cagccgucuc cgcaugagga cuagugcauc     2160

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     2220

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     2280

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2340

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2460

cagagccacc agaauu                                                     2476


<210>  7247
<211>  2473
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7247
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaga       60

agaaaagaag aggagcagac accagcguag gcaucguagg ccuccuacuc acaaccgcaa      120

uggccgccga aguaacccgc cggggcuccg cauacuacau guaccuagac cgcaaugaug      180

ccggcgaagc aauaucguuc cccaccaccc ucggcaugaa caaaugcuac auacaaauca      240

uggaccuagg acacaugugc gacgccacca uguccuacga augccccaug cuagacgaag      300

ggguugaacc cgaugacguc gacugcuggu gcaauacaac gagcaccugg gugguuuacg      360

gaacgugcca ccacaagaaa ggggaggccc gccgcucccg cagagccgua acccuccccu      420

cacacagcac cagaaaacua cagacacgaa gccagacaug gcucgaauca cgagaauaca      480

ccaagcaccu cauccgcgua gaaaacugga uauuccgcaa ccccggcuuc gcccuagccg      540

cagccgccau cgccuggcuc cuuggcuccu caacaagcca aaaagucaua uaccucguca      600

ugauucuacu caucgcgccc gccuacucca uccguugcau cggagucucc aacagagacu      660

ucgucgaagg caugucagga ggcaccuggg uugacaucgu ccuugaacac ggggguugcg      720

uaacggugau ggcccaggac aaacccacag uagacauaga acuugugacc acaacuguau      780

ccaacauggc ggaaguccgc ucauacugcu acgaagccag caucucggac auggccuccg      840

acucccgaug cccaacacaa ggcgaagcau accucgacaa acaauccgac acccaauacg      900

ucugcaaacg gacacucguc gaccgcggau ggggaaacgg cuguggucua uucggcaaag      960

gaucccuagu gaccugcgca aaauuugcuu gcucaaagaa aaugacggga aaaucaaucc     1020

agcccgaaaa ccucgaauac agaaucaugc uuuccgucca cggcagccaa cacagcggaa     1080

ugauagucaa cgacaccggc caugaaaccg acgaaaaccg ggccaaagua gaaaucaccc     1140

caaacucacc ccgcgcagaa gccacccucg gaggguucgg aagccucgga cucgacugcg     1200

aaccacgcac uggacuagac uucucagacc uguacuaucu cacgaugaac aauaaacauu     1260

ggcuaguaca caaagagugg uuccacgaua uaccacuacc auggcacgcc ggagcagaca     1320

caggcacacc ccacuggaac aacaaagaag cccucguaga guucaaagac gcacacgcca     1380

aacgucaaac cgucguagua cucgguucac aagagggcgc uguacacacc gcacuagccg     1440

gcgcccucga agccgaaaug gauggagcca aagggcgccu auccucaggc caccuaaaau     1500

gccgacucaa aauggacaaa cuucgccuca aagggguauc auacucccua ugcacagccg     1560

ccuucaccuu cacaaagaua cccgccgaaa cauuacacgg caccgucaca gucgaggucc     1620

aauacgccgg caccgacgga cccugcaaag ucccggcaca gauggcagua gacaugcaaa     1680

cccucacacc cgucggccgc cuaauaacag caaaccccgu caucaccgaa ucaaccgaaa     1740

acagcaaaau gaugcuggaa cucgacccac ccuucggcga cuccuacauc guaaucggcg     1800

uaggcgaaaa gaaaauaacc caucacuggc acagguccgg uagcaccauc ggaaaagcau     1860

ucgaagcaac cguccgaggc gccaaacgga uggcuguacu aggagacacu gccugggacu     1920

ucggcuccgu uggcggcgcc cuaaacucgc uaggcaaagg aauccaccaa aucuucggcg     1980

ccgccuuuaa aagccuauuc ggaggaauga gcugguucuc acaaauccuc aucggaacac     2040

uacuaaugug gcugggacug aacaccaaaa acggaagcau aucacuuaug ugccucgcac     2100

uagguggcgu ucuuaucuuc cucagcacag cgguaucugc augaggacua gugcaucaca     2160

uuuaaaagca ucucagccua ccaugagaau aagagaaaga aaaugaagau caauagcuua     2220

uucaucucuu uuucuuuuuc guugguguaa agccaacacc cugucuaaaa aacauaaauu     2280

ucuuuaauca uuuugccucu uuucucugug cuucaauuaa uaaaaaaugg aaagaaccua     2340

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2460

agccaccaga auu                                                        2473


<210>  7248
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7248
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaga       60

aacggcgagg cgccgacaca agcgucggca ucguaggccu acuccuaacc accgcuaugg      120

cagccgaagu aacacgccga ggaagcgcau acuauaugua ccuagaucgc aacgacgcag      180

gcgaggccau cagcuucccc accacauuag gcaugaauaa augcuacauc caaauaaugg      240

aucuaggcca caugugcgau gccacaauga guuacgaaug cccaaugcuc gacgagggag      300

ucgaacccga cgacguagac ugcuggugca acaccacuuc aacauggguc guauacggaa      360

ccugccacca caagaaaggc gaagcaagga gaucaaggag agcugucacg cuccccucac      420

acagcacccg caaacuacaa acacgguccc aaaccuggcu ggaaagccga gaguauacaa      480

aacaccuaau ccgcgucgaa aauuggauau ucagaaaccc cggcuucgcg cucgcagcag      540

ccgcaauagc auggcuacuc ggcucaagca cuucacaaaa agucaucuau cuagucauga      600

uccuucucau cgcaccagca uacaguauac gcugcauagg gguaagcaac cgggacuucg      660

uugaaggaau guccgggggc acguggguag acauaguacu agaacacgga ggaugcguaa      720

cgguaauggc ccaagacaaa cccacagugg acaucgagcu aguaacgaca acagucagca      780

acauggccga gguacgguca uacugcuacg aggcaagcau aucagacaug gcauccgacu      840

caaggugccc cacacagggc gaggccuacc ucgacaagca aagcgacaca caauacgucu      900

gcaaacggac ccucguagau agaggcuggg gcaacggaug cggccucuuc ggaaaaggga      960

gccuaguaac augcgcaaaa uucgccugca gcaagaaaau gaccggaaaa agcauacaac     1020

cagaaaaccu ugaauaccgc auaaugcuaa gcguacacgg cagccaacac agcgguauga     1080

ucguaaacga cacaggacac gaaaccgacg aaaauagagc aaaaguagaa auaacaccga     1140

acuccccucg ggcagaagcc acacucgggg gauucggaag ccuaggccua gacugcgagc     1200

ccagaaccgg acuggacuuc agcgaccucu acuaccuaac aaugaacaac aagcacuggc     1260

uaguacacaa agaaugguuc cacgacauac cucucccaug gcacgcaggg gcagacacag     1320

gcacacccca cuggaacaau aaagaagccc uaguggaguu caaagacgcc cacgcaaaaa     1380

gacaaaccgu aguaguccuc ggaagccaag aaggcgccgu ccacaccgcg cuagccgggg     1440

cgcuggaagc ggaaauggac ggcgccaaag gacgacuaag cagcggccac cuaaaaugcc     1500

gacuaaaaau ggacaaacua aggcucaaag gcguauccua cucacucugc acagccgccu     1560

ucaccuucac aaaaauacca gcagagacgc uccacggcac agugacggug gaaguccaau     1620

augccggcac agacggacca ugcaaggugc cagcacaaau ggcggucgac augcaaacac     1680

uaacacccgu aggccgccua auaaccgcca accccguaau caccgaaagc acggaaaauu     1740

cgaaaaugau gcuagagcuc gacccaccgu ucggagacuc auacaucguc auaggagugg     1800

gcgagaaaaa gauaacacac cacuggcacc gaagcggcuc cacaaucgga aaagcauucg     1860

aagccacagu aagaggggcc aagagaaugg ccguacucgg agauacagca ugggauuucg     1920

ggucaguagg cggagcccua aacagccuag gcaaaggcau ccaccagauc uucggagcag     1980

cauucaaaag ucucuuugga ggaauguccu gguucuccca aauacugauc ggcacacuac     2040

ugauguggcu gggacuaaac accaaaaacg ggagcaucuc acucaugugu cuggcacugg     2100

gcggaguacu caucuuccua agcacagccg uaucagcaug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  7249
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7249
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaaaa       60

gacgcggcgc ggacaccucc gucggcauag ucggucuucu gcucacaaca gcaauggccg      120

cagaggucac acgaagaggc agcgccuacu acauguaccu cgaccgaaac gacgcaggcg      180

aagcaaucuc auuccccaca acccuaggca ugaacaaaug cuacauccag auaauggacc      240

uaggccacau gugcgacgcc acaauguccu acgaaugccc uauguuagac gaaggaguug      300

aaccagacga cguagacugc uggugcaaca caacaucaac guggguggug uauggcacau      360

gccaucacaa gaaaggagaa gccagacgca gcagacgagc agucacccua cccagccacu      420

caacacgaaa acuccaaaca cggucacaga cauggcucga aagccgcgaa uacacgaaac      480

accuaauccg ggucgagaau uggaucuucc gcaauccagg auucgcccuc gcagccgcag      540

ccaucgccug gcuacuagga agcuccaccu cacaaaaagu caucuaccuc gucaugauac      600

uccuaauagc accagccuac uccaucagau gcauaggcgu cagcaaccgc gacuucguag      660

aaggaaugag uggcggcacc ugggucgaca ucguccuaga acacggcgga ugcguaaccg      720

ugauggcgca ggacaaaccc accguggaca uagagcuagu caccaccacc gucucgaaca      780

uggcagaagu acgauccuac ugcuacgaag ccagcauaag cgacauggca agcgacaguc      840

gcugccccac ccaaggagaa gccuaccuag auaaacaaag cgacacccaa uacgucugua      900

aacgaacccu agucgaccgc ggauggggca acggcugugg acucuucggg aaaggcucac      960

uggugacaug cgccaaauuc gcgugcucua agaaaaugac agggaaaucu auccaacccg     1020

aaaaccuaga auaccgcauc augcuaagcg uacacggcuc acaacacucc ggcaugauag     1080

uaaacgacac aggacacgaa accgacgaga acagagccaa aguagaaauc acgcccaacu     1140

cccccagggc cgaagcaacc cucggcggcu ucggaucccu ugggcuagac ugcgaaccca     1200

gaaccggacu cgacuucagc gaccucuacu accuaacaau gaacaacaaa cacugguuag     1260

ugcacaagga augguuccac gacaucccac uaccauggca cgcgggagca gacacuggca     1320

caccccacug gaacaacaaa gaagcucucg ucgaguucaa ggacgcccac gccaaaaggc     1380

aaacagucgu cguccuaggg agccaggaag gagccgugca caccgcacuc gcuggcgccc     1440

uggaagccga aauggacgga gccaaaggca gacuaucaag cggacacuua aaaugcagac     1500

uaaaaaugga uaaacuccgu cuaaaaggcg uaagcuacag ccuaugcacc gccgcguuca     1560

cauucaccaa aauaccagca gaaacucucc acggcaccgu gacaguagaa guccaguacg     1620

ccggcacgga uggaccaugu aaaguaccgg cccaaauggc aguagacaug caaacacuca     1680

cacccguagg acgccuaauc accgcaaacc ccgugauaac cgaaucaacc gaaaacagca     1740

aaaugaugcu cgaacucgac ccaccauuug gagauagcua caucgugauc gggguaggag     1800

agaaaaagau cacgcaccau uggcaccgcu caggcucaac cauaggaaag gccuucgaag     1860

caaccguacg aggagcaaaa cgcauggcag uacuaggaga cacugccugg gacuucggcu     1920

caguaggcgg agcacucaac ucccuaggca aaggaauaca ccaaaucuuc ggcgccgccu     1980

ucaaaagccu cuucggcggu auguccuggu ucagccaaau ccugaucggc acacuucuaa     2040

ugugguuagg acucaacacc aaaaacggcu ccaucucccu aaugugccua gcacucggcg     2100

gcguacucau cuuccuaagc acagcagugu ccgcaugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  7250
<211>  2464
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7250
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagac       60

gaggcgccga cacaucaguu ggaauaguag gacuacuacu aaccacagcc auggccgcag      120

aaguaacaag acgcggaucc gccuauuaca uguaccuaga ccgaaaugac gcaggcgagg      180

cgaucagcuu cccaacaacg cugggaauga acaaaugcua cauacaaaua auggaccuug      240

gccacaugug ugacgcaaca augagcuacg aaugccccau gcucgacgag ggcgucgaac      300

cagacgacgu agacugcugg ugcaauacaa caagcaccug gguagucuac ggaacaugcc      360

accacaaaaa gggagaagca cgacgcagcc gccgagcggu aacccucccc agccauucga      420

cccggaaacu acagacccga agccaaaccu ggcuagaauc acgcgaauac accaaacacu      480

uaaucagagu ggagaacugg aucuuccgca accccggauu cgcacuagca gcagccgcaa      540

uagcauggcu ccuaggcagc agcacaagcc aaaaggucau auaccucgug augaucuuac      600

ucauagcccc agccuacagc auacgaugca uaggcguguc caacagagac uucgucgaag      660

gcaugagcgg aggcacaugg guagacauag uacucgaaca cggcggaugc guaacgguga      720

uggcccaaga caaaccaaca guagauaucg aacuggucac caccacaguc agcaauaugg      780

cagaaguaag auccuacugc uacgaagcau ccauaagcga cauggcaagc gauagcagau      840

gccccacaca gggcgaagcc uaccucgaca aacaaucaga uacacaauac gucugcaagc      900

gcacccuggu cgacagaggc uggggaaaug gaugcggccu auuuggaaaa gggucccugg      960

ucacgugcgc aaaguucgcg ugcagcaaga aaaugaccgg caaaagcauc caacccgaga     1020

accucgaaua cagaauaaug cucagcgugc acgggagcca acacagcggc augauaguaa     1080

acgacacugg acacgaaacc gaugagaacc gagccaaagu cgaaauaacc ccuaacucac     1140

cacgagcaga agcgacacua gggggcuucg gcagucucgg gcuagacugc gagcccagaa     1200

ccggccucga cuuuuccgac cucuacuacc uaaccaugaa caacaaacac uggcucgugc     1260

acaaagaaug guuccacgac aucccacucc cauggcacgc aggagcagac acaggaacac     1320

cccacuggaa caacaaagag gcacucgucg aguucaagga cgcccacgca aaaagacaaa     1380

caguaguagu ucucggaagc caagaaggag caguacacac cgcccuugca ggggcccucg     1440

aagccgagau ggacggagcg aaaggccggc uaucgucagg ccaccucaaa uguagacuaa     1500

agauggacaa acucagacua aaaggagugu cauacagccu gugcacagcc gcguucacau     1560

ucacaaaaau accggcggaa acacuccacg ggacggucac cguagaggug caauacgccg     1620

gcaccgacgg ccccugcaaa gucccggcac agauggccgu cgacaugcaa acccucacac     1680

caguaggccg acuaaucaca gccaacccgg uaauaacgga auccacagaa aacucaaaaa     1740

ugaugcuaga acucgaccca ccauuuggcg acagcuacau agugauaggg guaggcgaaa     1800

agaaaaucac acaccacugg caccgaagcg gcagcaccau agggaaagca uucgaagcaa     1860

ccguacgggg agcaaaacgc auggcaguac ucggggacac agccugggac uucggcagcg     1920

uaggcggugc acuaaacuca cuaggcaagg gcauacacca aauauucggg gcggccuuca     1980

aaucucuauu cgggggcaug agcugguucu cacaaauccu aaucgggacc cuccucaugu     2040

ggcuaggacu caauacaaag aacggcagca uaucgcucau gugccuggca uuaggaggag     2100

uccuaaucuu ccucagcaca gcaguaagcg caugaggacu agugcaucac auuuaaaagc     2160

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2220

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2280

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2460

aauu                                                                  2464


<210>  7251
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7251
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gagcagacac cagcgucggc aucgugggcc uccuucugac aacagccaug gccgcggagg      120

uaacacgaag aggcagcgcg uacuacaugu accuagacag aaaugacgca ggagaagcca      180

ucucguuccc aacaacccua ggaaugaaca aaugcuacau ccaaaucaug gaccuaggcc      240

acaugugcga cgccaccaug agcuacgaau gcccaaugcu cgacgaagga guagagcccg      300

acgacguaga cugcuggugc aacaccacca gcacaugggu aguauacgga accugccacc      360

acaaaaaggg ggaagcucgc cgaagccgcc gcgcugucac acuccccagc cacagcacca      420

gaaaacucca aacccgcuca caaaccuggc uagaaagccg agaauacacc aaacacuuaa      480

uccgagugga aaacuggaua uucaggaauc ccggcuucgc ccuugccgcc gccgcaauag      540

ccuggcuccu cggcagcuca accucacaaa aagucauaua ccuagucaug auacuccuaa      600

uagcccccgc cuacuccaua agaugcauag gcguaagcaa cagagacuuc guagaaggga      660

ugucuggcgg caccugggua gacauuguac ucgaacacgg cggcugugug acagugaugg      720

cccaagacaa accaaccgua gacaucgagc uagucacaac caccgucucc aacauggccg      780

aagucagauc cuacugcuac gaagcgagca uauccgacau ggcguccgac agccgaugcc      840

ccacacaggg cgaagccuac cucgacaaac aaagcgacac ccaguacgua uguaaacgca      900

ccuuagugga cagaggaugg ggcaacggcu gcggacucuu cggcaaggga ucccucguca      960

caugcgccaa auucgcaugc ucgaagaaaa ugaccggcaa aagcauccaa ccagaaaacc     1020

ucgaauaccg aauaaugcuc ucgguacacg gcagccaaca cagcggaaug aucguuaacg     1080

acaccgggca cgagacagac gaaaaccgug ccaaagucga aauaaccccc aacagcccaa     1140

gagcagaggc cacccucggc ggauucggua gccuaggacu agacugcgag cccagaaccg     1200

gccuggacuu cucagaccuc uacuaccuaa cuaugaacaa caaacacugg cuaguccaca     1260

aagaaugguu ccacgacaua ccccuacccu ggcacgccgg agcagacaca ggcaccccac     1320

acuggaacaa caaagaagcc cuaguagagu ucaaggacgc acacgcaaaa cgccaaacag     1380

uuguaguccu uggcagccaa gaaggagccg uccacaccgc ccucgcagga gcccuagaag     1440

cagaaaugga uggcgcgaaa ggccgccuau cauccggcca ccuaaaaugc agacucaaga     1500

uggacaaacu aagacucaag ggagucuccu acucccuaug uaccgcagcc uucacguuca     1560

ccaaaauccc agcagaaaca cuccacggca ccguaaccgu cgaaguacaa uaugccggua     1620

cagacggacc cugcaaggua ccagcccaaa uggccgugga caugcaaacc cuaacacccg     1680

uaggccgccu gaucacagcg aaccccguua ucaccgaauc cacagaaaac ucaaagauga     1740

ugcucgaacu agacccaccc uucggagacu ccuacauagu caucggcguc ggggaaaaga     1800

aaauaacaca ccacuggcac cgcuccggca gcaccauagg uaaagcauuc gaggccaccg     1860

uacgaggagc caaacgaaug gccguacuag gcgacaccgc cugggacuuu ggauccgugg     1920

gcggcgcacu aaacucacua ggcaaaggaa uacaccaaau cuucggggca gccuucaaaa     1980

gccuauucgg cggaaugucu ugguucucac aaauccucau aggaacacuc cugauguggc     2040

ucggacugaa caccaaaaac ggauccauaa gucuaaugug ccuagcacug ggcggagucc     2100

uaaucuuccu gagcaccgca guauccgcgu gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  7252
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7252
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacacauc ggucggaaua gucggacuac uccucacaac agccauggca gcggaaguca      120

cgcgaagagg cuccgccuac uacauguacc ucgaccgcaa cgacgcaggg gaagcgauca      180

gcuuccccac cacccucggg augaacaaau gcuacauaca aauaauggau cuaggacaca      240

ugugcgacgc gacgaugagc uacgaaugcc ccaugcuaga cgaaggagua gaaccagacg      300

acgucgacug uuggugcaac acaacaucca cuuggguggu auauggaacc ugccaucaca      360

agaaaggaga agccaggagg ucccgcagag ccgucacacu ccccagccac uccacucgca      420

agcuacaaac aagaucacaa accuggcuag aaagcaggga auauacaaaa caccuaauaa      480

gaguggaaaa cuggaucuuc agaaaccccg gcuucgcccu agccgcagcg gccauagccu      540

ggcuacucgg cagcuccaca agccagaaag ucaucuaccu cguaaugauc cuucugaucg      600

ccccagcgua cuccauacgg uguauaggag uauccaaccg cgacuucguc gaaggcaugu      660

caggcggaac augggucgac auaguccucg aacacggugg augcgucacg guaauggcac      720

aggacaaacc cacggucgau aucgagcuag uaacaaccac ggucuccaac auggccgaag      780

uccgcucaua cugcuacgaa gccuccauau ccgacauggc guccgacucc cgcugcccaa      840

cccaaggcga agccuaccuc gacaaacaau ccgacaccca auacguaugc aaacgaaccu      900

uagucgaccg cggauggggg aacggaugug gccuauucgg caagggcucg cucguuacau      960

gcgccaaauu ugccugcucg aagaaaauga cgggaaaaag cauccaaccc gaaaaccuag     1020

aauacagaau aaugcuaucg guccauggau cacaacacuc agggaugaua guaaacgaca     1080

cgggacacga gaccgacgaa aaccgcgcga aagucgaaau uaccccaaac aguccucgcg     1140

cagaagcaac ccuaggcggg uucggaagcc ucggccucga cugcgaacca cggacaggcu     1200

uagacuuuag cgaccuguac uaccucacca ugaacaacaa acacugguua guccacaaag     1260

aaugguucca cgacauaccc cuaccauggc acgccggagc cgauacaggu acaccccacu     1320

ggaacaacaa ggaagcgcua guggaguuca aagacgccca cgcaaaacgc caaaccgucg     1380

ucgucuuagg cagccaagaa ggagccgucc acaccgcgcu cgccggagcc cuagaagcag     1440

agauggacgg agcaaaagga cgccuaaguu cagggcaccu aaagugccga cugaaaaugg     1500

acaagcugcg ucucaaaggc guguccuacu cacuaugcac ggcggcauuu acguucacca     1560

aaauccccgc agaaacccua cacggaaccg ucaccguaga aguccaauau gccggaacgg     1620

acggacccug caagguccca gcccaaaugg ccgucgacau gcagacccug acccccguag     1680

gccggcuaau aaccgcaaac ccgguaauaa cagaaucaac agagaacuca aaaaugaugc     1740

ucgaacugga cccacccuuc ggcgacuccu acauagucau cggaguagga gaaaagaaaa     1800

uaacccacca cuggcaccgc agcggaagca ccauaggcaa agcguucgag gcgaccgucc     1860

gcggcgccaa aaggauggca guccuaggag acaccgcaug ggacuucgga agcguaggag     1920

gcgcccucaa cucacuagga aaaggaaucc accaaaucuu cggagcggcg uucaaaagcc     1980

ucuucggcgg aaugagcugg uucucacaaa uccucaucgg aacccugcua auguggcucg     2040

gacuaaacac caaaaacggg agcaucuccc uaaugugucu cgcacuaggu ggcguccuca     2100

ucuuccucag cacagcagua ucagcgugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  7253
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7253
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggccg       60

acacaagcgu aggaauagua ggacugcucc ugaccacagc aauggcagca gaaguaacaa      120

gacgaggcuc cgcguauuac auguacuuag accgaaacga cgccggagaa gccauaucuu      180

uccccaccac ccuagggaug aacaagugcu acauucaaau aauggacuug ggccacaugu      240

gcgaugcgac caugagcuac gagugcccca ugcuagacga gggcgucgaa ccagacgacg      300

uagacugcug gugcaacacg accucaacau ggguaguaua cgggacaugc caccacaaga      360

aaggagaggc acgaagaagc cgacgagccg uaacgcuacc cucccacucg acacgcaaac      420

uccaaacaag aucccaaaca uggcuggaaa gcagggaaua cacaaaacac cucauuagag      480

uagaaaacug gauauuucga aacccaggcu ucgcccuagc agcagcagcg aucgccuggc      540

uccucggaag cagcaccucg caaaaaguaa uuuaccucgu aaugauacua cuaaucgcgc      600

cagcauacag caucaggugu aucggaguaa gcaacagaga cuucguagaa ggcaugucag      660

gaggaaccug gguagacauc guacuagaac acggaggaug cguaaccgug auggcucaag      720

acaaaccaac cguagacauc gagcuaguaa ccacuacagu cuccaauaug gcugaaguca      780

gauccuacug cuacgaagcc ucaauuagcg acauggcgag cgacagccga ugcccgaccc      840

aaggagaagc cuaccucgac aaacaaucgg acacccaaua cguaugcaaa aggacacugg      900

ucgaccgagg cugggggaac ggcugcggcc uguucgggaa aggcagccuc guaacaugcg      960

cgaaauucgc cugcuccaag aaaaugacug gaaaaucaau ccaaccagaa aaccuggaau     1020

acagaaucau gcucuccgua cacgggagcc aacacagcgg aaugaucgua aacgacaccg     1080

gccacgaaac cgacgaaaac cgcgcgaaag ucgaaauaac gcccaacuca cccagagccg     1140

aggccacacu aggagguuuc ggaucacuag ggcucgacug cgaacccagg accggacuag     1200

acuucagcga ccucuacuac cucacaauga acaacaaaca cuggcuagug cacaaagaau     1260

gguuucacga cauaccccua cccuggcacg caggcgcaga caccggcacg ccccacugga     1320

acaacaaaga agcacucguc gaguucaaag acgcccacgc caaacgccag accguagucg     1380

uccuaggaag ccaagaagga gcaguccaca ccgcccuagc aggggcucua gaagccgaaa     1440

uggacggcgc aaagggacga cucagcagcg gccaccuaaa augccgccug aaaauggaca     1500

aacucagacu caaaggagua ucauacucac uaugcacagc agcauucaca uucaccaaaa     1560

uccccgcaga aacccuccau ggaacaguca cagucgaagu ucaauaugcc ggcacagacg     1620

gaccaugcaa aguacccgca caaauggccg uagacaugca gacgcuaacc cccgucggaa     1680

ggcucauaac agcuaacccc gucauaaccg aaagcacaga aaacuccaaa augaugcucg     1740

aacucgaucc cccguucggc gacucguaca uugucauagg cgucggagaa aagaaaauca     1800

cacaccacug gcaccgcuca ggcagcacaa ucggcaaagc cuucgaagcg acaguacggg     1860

gagccaaacg aauggccguc cucggagaca cagccuggga cuucggaagc gucggaggag     1920

cccuuaacag ucugggaaaa ggaauccacc aaaucuucgg ggcagcuuuc aaaagccucu     1980

ucggcggcau gagcugguuc agccaaaucc uaaucggaac cuuacuaaug uggcucgggc     2040

ugaacacgaa aaacgggucc auaagccuca ugugccucgc ccugggcgga guccucauau     2100

uccuaagcac agccgucucc gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  7254
<211>  2452
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7254
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggaca       60

caagcgucgg aauagugggu cuccuacuca cgaccgcaau ggccgcagag guaacccgaa      120

ggggcucugc auacuacaug uaccuggacc gcaacgacgc aggcgaagcc auaucauucc      180

ccacgacacu cggaaugaac aaaugcuaua uacagaucau ggaccucgga cauaugugcg      240

acgccaccau guccuacgaa ugccccaugc uagacgaagg cguggaacca gacgacgucg      300

acugcuggug uaacaccacg agcaccuggg uagucuacgg cacaugccac cacaagaaag      360

gggaagccag aagaucacgc cgagcgguaa cgcucccguc acacuccaca agaaaacuac      420

agacacgaag ccaaaccugg cucgaaagcc gcgaauacac caagcaccua auaagaguag      480

agaacuggau uuuccgaaac ccuggguucg cgcuagcagc ugcagcaaua gccuggcucc      540

uagggagcuc aacgucacag aaagucaucu accucgucau gauacuacug aucgcacccg      600

ccuacuccau ccgaugcaua ggcgucucaa accgcgacuu cguagaaggc auguccggag      660

ggaccugggu cgauaucguu cucgaacacg gaggaugcgu aacagucaug gcccaggaua      720

aacccacagu agacauagaa cucguaacaa cgacagucuc aaacauggcc gaaguaaggu      780

ccuacugcua cgaagcauca aucucggaca uggccuccga cucacgaugc cccacccaag      840

gcgaagccua ccucgacaaa caaucagaca cccaauaugu cuguaaacgg acccucguag      900

accgcggcug gggcaacgga ugcggccuau ucggcaaagg cucccuagug accugcgcca      960

aauucgcaug cucaaagaaa augacaggca aaagcauaca acccgaaaac uuagaauacc     1020

guaucaugcu uuccguacac ggcagccaac acuccggcau gaucgucaau gacaccggcc     1080

acgaaacuga cgaaaauaga gcaaaaguag aaaucacacc aaacagccca cgagcggaag     1140

cgacgcucgg cggcuuuggc ucacucggcc ucgacuguga gccaagaaca ggacuagacu     1200

ucuccgaucu auacuaccuc acgaugaaca acaaacauug gcuaguacac aaagaauggu     1260

uucaugauau cccccuaccu uggcacgccg gagccgacac aggaacaccu cacuggaaca     1320

auaaagaggc acucguagag uucaaagacg cacacgccaa aagacaaacc guagucgucc     1380

uuggcucaca agaaggcgcc guacacaccg cccucgccgg agcacucgaa gccgagaugg     1440

acggcgccaa aggccgacua ucuagcggcc accucaagug caggcucaag auggauaaac     1500

uacgacucaa gggaguauca uacucccuau gcaccgcagc auucacguuc acaaaaauac     1560

ccgcagaaac ccuacacggc acagucacag uggagguaca guacgccggc acagacggac     1620

cauguaaagu cccagcacag auggccguug acaugcagac ccuaaccccc gucgggagac     1680

ucaucacagc aaacccagua aucacagaaa gcacagaaaa cucaaagaug augcucgaac     1740

uugacccacc cuucggcgac uccuacaucg ucauaggagu aggagagaag aaaauaaccc     1800

accacuggca ccgcuccgga aguacaauag gcaaagcauu cgaagccacc guccgaggcg     1860

caaaacgcau ggcgguccua ggcgacaccg cgugggacuu cggaagcguc ggcggcgcac     1920

ucaacucucu gggaaaaggc auacaccaga uuuucggcgc agccuucaaa ucccuauucg     1980

gcggcaugag cugguucagc caaauacuca ucggcacucu ccuaaugugg cuaggccuca     2040

acacgaaaaa cggcagcaua agccuaaugu gccucgcccu aggaggaguc cuaauauucc     2100

uaucaacagc agugagcgcu ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2160

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2220

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2280

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2400

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2452


<210>  7255
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 2 opt

<400>  7255
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucgggau cgucggccuc cuacuaacca cagcaauggc agcagaagua accaggcgcg      120

guuccgccua cuauauguac cucgacagaa acgacgcggg cgaagccauc uccuuuccaa      180

ccacacucgg caugaacaaa ugcuacaucc aaauaaugga ccucggacac augugcgacg      240

ccacgauguc cuacgaaugu cccaugcuag acgaaggugu cgaaccagac gacguagacu      300

gcuggugcaa caccaccagc accugggugg uauacggcac augccaccac aagaaaggcg      360

aagccagacg aucgcgccga gccgucacgc uccccuccca cucgaccaga aaacuacaaa      420

cccgcucuca gaccuggcuc gaaucgcgag aauauaccaa acaccuuaua cgagucgaaa      480

acuggauauu ccgcaaccca ggauuugcau uagccgccgc agcaaucgcc uggcuacucg      540

ggagcucaac aucacaaaaa guaauuuacc ucgucaugau ccugcucauu gccccagcuu      600

acucaauacg auguaucggg guaagcaacc gagacuuugu cgaaggcaug agcggaggga      660

ccugggucga caucguccuc gaacacggcg gcugcguaac agucauggcc caggauaaac      720

ccaccgucga cauugagcua gucacaacca cagucucgaa cauggccgaa guacgcagcu      780

acugcuacga agcguccaua agugacaugg ccagcgacuc ccgcugcccc acucaaggcg      840

aagccuaccu cgauaaacaa agcgacaccc aauacguaug caaaagaacc cucgucgacc      900

gaggcugggg caacgguugc ggccuauucg gaaaaggcuc ccuaguuacc ugcgcaaagu      960

ucgcaugcuc aaagaaaaug accggcaaau ccauccaacc agaaaaccug gaauaccgca     1020

uaaugcugag cguccacggc ucccaacacu ccggaaugau cgucaacgac acagggcacg     1080

aaacggacga aaaccgcgca aaaguugaaa uaacccccaa uagcccccgc gcagaagcaa     1140

cacucggcgg cuucggaagc cuagggcucg acugcgaacc ccgcacaggc cucgacuucu     1200

ccgaccugua cuaccuaacc augaacaaca aacacuggcu cguccacaaa gaaugguucc     1260

acgacauccc acuaccaugg cacgcgggug cagacacagg cacaccccac uggaauaaca     1320

aagaagcccu cguagaguuc aaagaugccc acgcaaaaag acagaccguc guaguccucg     1380

gcagucaaga aggcgccguc cacacggcuc ucgccggagc ccuagaagcc gaaauggacg     1440

gagcaaaagg aaggcuaucc ucaggccacc ucaaaugcag gcucaagaug gacaaacuaa     1500

gacucaaagg cguaagcuac agccucugca cagccgcauu caccuucacg aaaauucccg     1560

cggaaacgcu acacggcaca gugacagucg aaguccagua cgcaggcaca gacgggccau     1620

gcaaaguacc cgcacaaaug gcggucgaca ugcagacacu cacccccgua ggaagacuua     1680

ucacagcaaa ccccguaauc acagaaucca cagaaaacag caaaaugaug cuagaacuag     1740

auccacccuu cggagacucc uacaucguaa uaggcgucgg agaaaagaaa aucacucacc     1800

auuggcacag aagcggcucc acaauaggga aagccuucga ggcgacagua cgcggagcua     1860

aacgcauggc cguacucggg gacaccgccu gggacuucgg cuccgucggc ggcgcacuaa     1920

acagucucgg caaaggaaua caccaaaucu ucggcgccgc cuucaaaucc cuauucggag     1980

gcaugagcug guucagccaa auccugauag gcacauugcu caugugguua ggccucaaca     2040

ccaaaaacgg cagcaucagc cucaugugcc ucgcacucgg gggaguacua auauuccuau     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7256
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7256
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguaggcau cgugggacuc cuccucacaa ccgcaauggc cgcagccgua accagacgcg      120

gcuccgcaua uuacauguac cucgacagaa acgacgccgg cgaagcgauc ucguuuccca      180

ccacccucgg gaugaacaaa uguuacaucc aaaucaugga ccucggccac augugcgacg      240

ccaccaugag cuacgaaugc cccaugcuag augaaggagu agaaccagac gacguagacu      300

gcuggugcaa caccacauca acauggguag uguacggcac augucaccac aagaaaggug      360

aagcacggcg cagcagacgc gcgguaacgc uccccucaca cuccacacgc aaacuccaaa      420

ccagaagcca aaccuggcua gaaagccgag aauacaccaa acaccucaua cgagucgaaa      480

acuggauauu cagaaacccc ggauucgccc ucgcggccgc ggcaauagcu uggcuacucg      540

gauccucgac cucacaaaaa gucaucuacc uggucaugau cuuacucaua gcaccagcau      600

acucgauccg guguaucggc gugucuaacc gcgacuuugu cgagggcaug uccggcggca      660

ccugggugga caucguccua gaacacgggg gcugcgucac cguuauggcc caagacaaac      720

ccacgguaga cauagagcuc gucaccacca caguauccaa cauggccgaa guccgaucau      780

acugcuacga agccuccaua agcgacaugg ccucagacuc ccgcugcccc acacaaggug      840

aagcauaccu agacaaacaa ucagacacgc aauacgugug caaacgcacu cuggucgacc      900

gaggcugggg caacggcugc ggccucuucg gcaagggaag ccuaguaacc ugcgccaaau      960

ucgccugcuc aaagaaaaug accggcaaau caauccaacc cgaaaaccuc gaauaccgaa     1020

ucaugcuauc aguccacgga ucacagcacu cuggcaugau agucaacgac acaggucacg     1080

aaacagacga aaaccgcgcc aaaguugaaa ucacccccaa cucaccucga gcagaagcca     1140

cccucggagg auucggcucc cuaggccuag acugcgaacc acgcacagga cuagacuuca     1200

gcgaccucua uuaccucacc augaacaaca aacauuggcu aguucacaaa gaaugguucc     1260

acgacauacc ccucccaugg caugccggug cagacaccgg aacaccucac uggaacaaca     1320

aggaagcccu cguagaguuc aaggacgcac acgcaaaacg acaaacaguc guaguccucg     1380

gaagccagga aggagccguu cacaccgcac uugcaggagc ccuggaagcc gaaauggacg     1440

gugccaaagg ccgccuuucc ucagggcacc ucaaaugccg ccucaaaaug gacaaacuac     1500

gccucaaagg cgucucuuac ucccucugua cugccgccuu uaccuucacc aagauaccag     1560

cagaaacucu gcacggcacc gucacagucg aaguacaaua cgcuggcacc gacggacccu     1620

gcaaaguccc cgcccaaaug gccgucgaca ugcagacccu aacccccgua ggaagacuaa     1680

ucacagccaa uccagucauc accgaaucca cagaaaacuc aaaaaugaug cucgaacucg     1740

auccacccuu cggagacagc uacauuguca ucggcgucgg ugaaaagaaa auaacacacc     1800

acuggcaucg aucaggcuca acgaucggaa aagcguucga agcuacagua cgaggcgcca     1860

aacggauggc cguccucggc gacaccgcuu gggacuucgg cuccgucgga ggugcacuca     1920

acucacucgg caaagguauc caccaaaucu ucggcgcagc guucaagagc cucuucggcg     1980

gcaugucaug guuuucacaa auacucauag gaacacuacu cauguggcuc gggcucaaca     2040

caaagaacgg cagcauaagc cucaugugcc ucgcacuugg cggcguccua aucuuccuau     2100

cgacagcagu guccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7257
<211>  2461
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7257
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gcgcggacac aucagucggc aucgucgggc uacuacucac gacggccaug gcagccgcug      120

ucacaagacg aggaagugcc uauuacaugu accucgaccg caacgacgcc ggggaagcca      180

ucuccuuccc cacaacacuc ggcaugaaca aaugcuacau ccaaaucaug gaccucggcc      240

auaugugcga cgccaccaug ucauacgagu gcccaaugcu agacgaagga gucgagccag      300

acgacgucga cugcuggugu aacacaaccu ccacaugggu aguuuauggc acgugucacc      360

acaaaaaggg cgaagcccgc cgaagccgca gagccgucac ccuaccuuca cacagcaccc      420

gcaagcuaca aacacgcagc caaaccuggc uggaaucacg agaauacaca aagcaccuaa      480

uacgcgucga gaacuggaua uuccgcaacc ccggcuucgc acuagcggcc gcagcgaucg      540

cauggcuccu cggcuccagc acaucgcaaa aggucaucua ccucguaaug auacuccuaa      600

ucgcacccgc guauagcaua cgguguauag gcgucagcaa ccgagacuuc guagaaggca      660

ugagcggugg uacauggguc gacaucgucc ucgaacacgg aggcugcguc acagucaugg      720

cacaagacaa gccaacagua gacaucgaac uuguaaccac caccguuagc aacauggcug      780

aaguuagauc auacugcuac gaagccagca uaagcgacau ggccucagac agccgaugcc      840

caacgcaagg agaagccuac cuagacaaac aaucggacac ccaauacguc ugcaaacgaa      900

cccuagucga ccgcggcugg ggaaauggau gcggccuauu cggaaaagga ucccucguca      960

caugcgccaa auucgcgugc ucaaagaaaa ugaccggcaa aagcauacaa ccagaaaacc     1020

uagaauacag aauaaugcuc ucaguccacg gcagccaaca cagcggcaug aucgucaacg     1080

acacaggcca cgaaaccgac gaaaacagag caaaagucga gauuacaccu aacaguccac     1140

gcgcugaagc cacccuaggc ggauucggau cgcuaggccu cgacugcgaa ccacguaccg     1200

gacuagacuu cuccgaccua uacuaccuca caaugaacaa caaacacugg cucguacaca     1260

aagaaugguu ccacgacaua ccccuacccu ggcacgccgg agcagacacc gggacaccac     1320

acuggaacaa caaagaagcc cucgucgagu ucaaagacgc ccacgcaaaa cgccaaacag     1380

ucgucguauu gggcucccaa gaaggggcag uccacacagc ccucgcggga gcccuagaag     1440

cagaaaugga cggcgccaaa ggacgacuaa gcuccggcca ccuuaagugc agacucaaaa     1500

uggacaaacu gcgacuuaaa ggagucucau acucacucug cacagcagcu uucacauuca     1560

caaaaauacc agcagaaacc cuucacggca cagugacggu agaaguccag uacgccggaa     1620

ccgacggccc cugcaaagua cccgcccaaa uggccguaga caugcaaacg cuaaccccag     1680

uuggacgucu cauaacagcu aacccaguaa ucaccgaauc gacugaaaac ucgaagauga     1740

ugcuagaacu cgacccacca uucggcgaca gcuacaucgu aaucggcguc ggcgagaaaa     1800

agauaacuca ucacuggcac cgcagcggua gcacaaucgg caaagccuuc gaagccacgg     1860

uaaggggagc aaaacgcaug gcaguccugg gggacaccgc augggauuuc ggaucggugg     1920

gcggggcccu aaacucacug ggcaaaggaa uccaccagau cuucggcgcc gcuuucaaau     1980

cacucuucgg cggcaugucc ugguucucac aaauacucau cggaacacua cucauguggc     2040

ucggacucaa caccaaaaac ggaagcauaa gccucaugug ccucgcacua ggaggagucc     2100

ucauuuuccu cucaaccgcc guuuccgcau gaggacuagu gcaucacauu uaaaagcauc     2160

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     2220

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     2280

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2400

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2460

u                                                                     2461


<210>  7258
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7258
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgcg       60

gccugagcag cagaaaacga cgaucacacg acguacucac aguccaauuc cucauacucg      120

gaaugcuacu caugaccggc ggggucacga gaagaggcag cgcauacuac auguauuuag      180

accgaaacga cgccggcgaa gccaucuccu uccccaccac acugggcaug aacaaaugcu      240

acauccaaau cauggaccua ggacacaugu gcgacgcgac caugucauac gaaugcccga      300

ugcucgacga aggcgucgaa ccagacgacg ucgauugcug gugcaacacc acauccaccu      360

ggguaguaua cggcacaugc caccacaaga aaggagaagc ccgacgcagc cggcgcgcug      420

ucacccuccc gucacacucu acccgaaagc ugcaaacccg aucacagacc uggcuggaau      480

cacgcgaaua cacaaaacac cucauccgcg ucgaaaacug gauauuccgc aaccccggcu      540

ucgcccuagc agcugcagcc aucgccuggc uacucggcuc cagcaccagc caaaagguca      600

ucuaccucgu aaugauccuc cucauagccc cagcauacuc aauccgaugc aucggaguga      660

gcaaccguga cuucgucgaa ggaauguccg gcgggacaug ggucgauauu gugcucgaac      720

acggaggcug cgucacagua auggcccaag acaaaccaac cguagacaua gagcucguua      780

ccaccaccgu auccaacaug gccgaagugc gcuccuacug cuacgaagca agcaucagcg      840

acauggccuc cgauucaaga ugccccacgc aaggcgaagc auaccuagac aaacaaucag      900

acacacaaua cgucugcaaa cgaacccucg ucgacagggg augggggaac ggcugcggcc      960

uuuuugggaa aggaucccua gucaccugug ccaaauuugc augcagcaaa aagaugaccg     1020

gaaaaucaau ucaaccagaa aaccucgaau accgaauaau gcucucaguc cacggcagcc     1080

agcacagcgg aaugaucgua aacgauacag gccacgaaac agacgaaaac cgcgccaaag     1140

uagaaauaac acccaacuca ccccgcgcag aagccacacu agguggauuc gggagccuag     1200

gacuagacug cgaaccccgc acaggucuag acuucuccga ccucuacuac cucacaauga     1260

acaacaagca cuggcuaguc cacaaagaau gguuccacga caucccgcua ccauggcacg     1320

ccggcgccga caccggaaca ccccacugga acaacaaaga agcccuaguc gaguucaaag     1380

acgcacacgc aaagagacaa acugucguag uccucggcuc ccaagaaggc gccguccaua     1440

ccgcacucgc aggcgcauua gaagccgaga uggacggcgc aaaaggacga cuaagcagcg     1500

gacaccucaa augccgccua aaaauggaca aacugagacu caaaggaguc ucuuacucac     1560

ucugcaccgc cgcauucacc uucacgaaaa uccccgccga aacccuccac gguaccguua     1620

ccgucgaagu ccaauaugca gggacagacg gcccaugcaa aguccccgcc caaauggcag     1680

ucgacaugca gacccucacg ccagucggca gacuaaucac agcaaacccc gucauaaccg     1740

aaagcacgga aaacuccaaa augaugcucg aacuagaucc cccauucggc gacucauaca     1800

ucgucaucgg aguaggugag aaaaagauca cccaccacug gcacagaagc ggaagcacga     1860

ucgguaaagc cuucgaagcc acuguccgag gugccaaacg cauggcugug cucggagaca     1920

ccgccuggga cuucgggagc gugggcggag cccuaaacag ccuagggaaa ggcauacacc     1980

agaucuucgg cgcagcauuc aagagcuuau ucggcgggau gagcugguuc ucacaaaucc     2040

ucaucggaac acuccucaug uggcuaggac ucaacaccaa aaacggaagc auaagucuga     2100

ugugccucgc ccucggagga guccuuauau uccuauccac agccgucagc gcgugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  7259
<211>  2485
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 2 opt

<400>  7259
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagag       60

gccuaucaag ccgcaaaaga cggucacacg acguauuaac aguacaauuc uuaauccuag      120

gcaugcugcu aaugacaggg gcaguuaccc gccgaggcuc agcguacuac auguaccugg      180

accguaacga cgccggcgaa gcaauuagcu ucccaaccac acucgggaug aacaaaugcu      240

acauccaaau aauggaccua ggacacaugu gcgacgccac aaugucguac gaauguccaa      300

ugcuagacga aggcgucgag ccagacgacg ucgacugcug gugcaauaca accagcacau      360

gggucguaua cggaaccugc caccacaaga aaggcgaagc ccgccgaucc cgccgcgcag      420

uaacccuccc cagccacagc acccgaaaac uccaaacccg gagccaaaca uggcuagaga      480

gcagggagua cacaaaacac cugaucaggg uagagaacug gauauuccga aaccccggau      540

ucgcacucgc cgccgcagcu aucgccuggc uacucggauc cuccaccucu caaaagguga      600

ucuaucuugu aaugauccuc cucauagcac ccgccuacuc aauacgcugc auaggaguua      660

gcaacagaga cuucgucgaa ggcaugagcg gcggaacaug ggucgacauc guccucgagc      720

acggcggaug cgucacaguc auggcccagg acaaaccaac cguagacaua gagcuaguaa      780

caacaacagu auccaauaug gccgaagucc gcuccuacug cuacgaggca ucaaucucag      840

auauggccuc agacucgcga uguccaaccc aaggggaagc cuaccucgac aagcagagcg      900

acacacagua cguauguaaa agaacccuug uugaccgagg cuggggaaac ggaugcggcc      960

uauuugguaa aggaucacuc guaaccugug ccaaauucgc augcuccaag aaaaugaccg     1020

gcaaaagcau acaacccgag aaccucgaau accgcauaau gcucagcgua cacggcagcc     1080

aacacagcgg gaugauagua aacgacaccg gacacgaaac cgacgaaaau cgagcgaaag     1140

uagaaauaac accaaacagc ccaagagccg aagccacccu cggcggauuc ggaagccucg     1200

gacuagacug cgagccgcgc acagggcucg acuucuccga ccucuacuac cucacaauga     1260

acaacaaaca cuggcucguc cacaaagaau gguuccacga cauaccacuc cccuggcacg     1320

ccggcgccga uaccggcacc ccacacugga acaacaaaga ggcccugguc gaguucaaag     1380

acgcgcacgc caagcgccaa acaguggucg uacucggauc acaagaaggc gcaguacaca     1440

cagcccucgc cggagcccuc gaagccgaaa uggacggcgc caaaggacgc cuaagcuccg     1500

gccaccucaa gugccgccuc aaaauggaca aauugcgacu caagggaguc agcuacucac     1560

ugugcacagc cgccuucaca uucacaaaaa uccccgccga aacacugcac ggaacaguca     1620

caguagaagu acaauacgca ggcacugacg gaccauguaa aguaccggcg caaauggcag     1680

ucgacaugca aacccucacg ccagucggac gacuaaucac cgccaaucca gucaucacag     1740

aaagcacaga aaacucaaaa augaugcuag aacuagaucc cccauucgga gacagcuaca     1800

ucgugauagg cgucggcgaa aagaaaauca cccaccacug gcaccgaagc ggcucaacca     1860

uaggcaaagc guucgaagcc accgucagag gcgcuaaacg gauggcagua cucggcgaca     1920

cggcauggga cuucgguuca gucgggggcg cgcuaaacag ccucggcaaa ggaauccacc     1980

aaauuuucgg ggccgcauuc aaaagccucu ucggcggaau gaguugguuc ucccaaauac     2040

uaaucggcac ccuccugaug uggcuaggcc uaaacaccaa aaacgguucg aucagccuca     2100

ugugccuagc guugggaggc gugcuaauau uccuaagcac agccguaagu gccugaggac     2160

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     2220

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     2280

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2340

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2460

ggcucuuuuc agagccacca gaauu                                           2485


<210>  7260
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 2 opt

<400>  7260
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaacgc aagaaaggaa aagaaacggc gcggcgcaga cacauccguc ggaauagugg      120

gacuccuacu cacaaccgca auggccgcgg aaguaacaag aagaggcuca gcguacuaca      180

uguaccuaga ccgcaacgac gcaggagaag caauauccuu ccccacaacc cucggcauga      240

acaaaugcua cauacagauc auggaccucg gacacaugug cgacgcaacc augagcuacg      300

aaugcccaau gcuagaugaa ggcgucgaac ccgacgacgu cgacugcugg ugcaacacca      360

ccuccacgug gguagucuau ggaaccugcc accacaagaa aggcgaagcc cgaagaucaa      420

ggagagcagu cacacuacca agccacagca cacgaaaacu acaaacacgu ucacaaaccu      480

ggcucgaauc cagagaauac acaaaacacc ucauacgagu cgaaaacugg auauucagaa      540

accccggcuu cgcccucgcg gcagccgcga uagcauggcu gcugggaagc aguaccucac      600

aaaaagucau auaccuagua augauccucc ucaucgcgcc cgccuacagc auucgcugca      660

uagguguaag caaccgggac uucguagaag ggaugagcgg cgguacaugg gucgacaucg      720

uacuagaaca cgggggcugc guuacaguaa uggcacaaga uaagcccacc guagacauag      780

aacucgucac aacaaccgua ucaaacaugg cugaggucag aagcuacugc uacgaagcau      840

ccaucuccga cauggcaagu gacagucgcu gccccaccca aggagaagcg uaccucgaca      900

aacaauccga cacccaauac gucugcaaac gaacacucgu agaccgcgga uggggaaaug      960

gcuguggacu uuucggaaaa ggcagccuag uaacaugcgc caaguucgcc ugcagcaaga     1020

aaaugacagg caaaucaauc caaccagaaa aucucgagua ccgcauuaug uuaucaguac     1080

acggcagcca acacagcggc augaucguca acgauaccgg gcacgaaacc gacgaaaaca     1140

gagccaaagu agaaaucaca ccaaacagcc caagagcaga agccacacuu gggggcuuug     1200

gcagccuagg ccucgacugc gaaccacgaa caggccucga cuucucagau cuauacuacc     1260

ucaccaugaa caacaaacac uggcucguac acaaagaaug guuccacgac aucccccucc     1320

ccuggcaugc cggggcagac acgggaacac cccauuggaa caauaaagaa gcccuaguag     1380

aauuuaaaga cgcccacgcc aaaagacaaa ccgucgucgu ccucggcagc caagaagggg     1440

ccguccacac cgcucuagcc ggggcccucg aagcagagau ggacggagcc aagggacggc     1500

uaagcuccgg ccaccuaaaa ugcagacuca agauggacaa acuaaggcuc aagggggucu     1560

cauacagcuu augcacagcc gcauucacau ucaccaaaau cccggcagag acauuacacg     1620

gcacagucac cgucgaagua caauacgcag gcacagacgg cccaugcaaa gucccagccc     1680

aaauggcggu agacaugcaa acacucaccc ccgucggccg ccuuaucaca gccaacccag     1740

ucaucaccga aagcacggaa aacagcaaga ugaugcucga acucgaccca cccuucggcg     1800

acucguacau aguaaucgga gucggagaga aaaagauaac ccaucacugg caccgcagcg     1860

gcuccaccau aggcaaagcc uucgaagcga ccguacgcgg cgcaaaacgg auggccguac     1920

ucggcgacac ugccugggac uucggaucag uaggcggcgc ccucaacuca cuaggcaagg     1980

gaauccacca aaucuuuggc gcagccuuca aaagccuauu cggcggaaug ucgugguuca     2040

gccaaauccu cauaggcacg uuacucaugu ggcucggccu aaacaccaaa aacggcucca     2100

ucucccuaau gugccucgcc uuaggcggag uccugaucuu ccucagcaca gcaguaucgg     2160

ccgacguagg augcuccgua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7261
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 2 opt

<400>  7261
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc gcgcaaagaa aagaaacgac gcggcgcaga cacaaguguc ggaaucgucg      120

gccuccugcu gacgacagcc auggcugcag aagucacacg cagaggaagc gccuacuaca      180

uguaccuaga ccgaaacgac gcaggcgaag ccauaucauu cccaacaacg cucggcauga      240

acaaaugcua cauucaaaua auggaccuag ggcacacaug cgacgcaacg augagcuacg      300

aaugccccau gcucgacgaa ggagucgaac cagacgacgu agacugcugg ugcaacacga      360

ccagcacgug ggugguauac ggaaccugcc accacaagaa aggggaagca cgaagaucac      420

gacgcgccgu gacccucccc agucacagca cacgaaaacu ccaaacacgc ucccaaacau      480

ggcuagaauc acgcgaguac acgaaacacc ucauccgagu cgaaaacugg aucuuuagaa      540

acccaggcuu ugcacucgca gcggccgcca uugcauggcu acugggcagu agcacauccc      600

agaaaguuau auaccucgua augauacuuc uaauagcgcc cgcauacucc auccgcugca      660

uaggaguguc caauagagac uucguagaag gaaugagcgg aggcaccugg guagacguag      720

ucuuagaaca cggaggcugc gucacaguca uggcccaaga caaaccgacc guagauauag      780

aacucguaac caccacaguc agcaacaugg cagaagugcg aucauacugc uacgaagccu      840

caaucuccga cauggccagc gacagccggu gcccaacaca aggagaagcc uaccucgaca      900

aacaaucaga cacccaauau gucuguaaac gcacacucgu cgacagaggg uggggaaacg      960

gcugcggccu cuucggcaaa gggucacucg ucacgugcgc aaaauucgca ugcagcaaga     1020

aaaugacggg caaauccauc caacccgaaa accucgaaua ccgaauaaug cucagcgucc     1080

acgggucaca acacagcgga augauaguca augacaccgg acacgaaaca gacgaaaaca     1140

gagcaaaagu agaaaucaca ccaaacucac cgcgagccga agccacacug ggaggauucg     1200

ggagucucgg ccucgacugc gaaccccgga cuggccuaga cuucuccgac cuauacuacc     1260

uaacgaugaa caacaaacau uggcucgucc acaaagaaug guuccacgac aucccacucc     1320

cguggcacgc cggcgccgac accggcacuc cccacuggaa caacaaagaa gcccuugucg     1380

aguucaaaga cgcccacgca aaacggcaaa caguaguagu cuuaggguca caggagggcg     1440

ccguucacac cgcccucgcu ggggcgcuug aggccgaaau ggacggcgcc aaaggccgcc     1500

ucuccagcgg acaccugaaa ugccgacuua aaauggacaa acuacgacua aaaggagucu     1560

ccuacagccu cugcacagca gccuucaccu ucacgaagau cccagccgaa acacuacacg     1620

gaacagucac aguagaagua caauacgcag ggaccgacgg cccaugcaaa guccccgccc     1680

aaauggccgu agacaugcag acacuaacgc ccguaggaag acucaucaca gcaaacccgg     1740

uaaucaccga aagcaccgaa aacuccaaaa ugaugcugga acucgauccc ccauucggag     1800

acagcuacau agucaucgga guaggagaaa agaaaauaac acaccacugg caccgcagcg     1860

gaucaaccau aggaaaagcc uuugaagcca ccguaagagg cgcaaaaagg auggcgguuc     1920

ugggggacac agcaugggac uucggcuccg uaggcggcgc acuaaacucc cucggcaaag     1980

gaauacacca aaucuucgga gcagccuuca aaucucucuu cggcggaaug agcugguuca     2040

gccaaauccu aauaggcacg cuacucaugu ggcuaggacu aaacgccaag aacggaucca     2100

ucagccuaau gugccuggcu cucggcggcg uguuaauauu ccuuagcaca gcagucuccg     2160

ccgacguagg cugcagcgua gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7262
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 2 opt

<400>  7262
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucauaaacgc gcgcaaagaa cgcaaacgaa gaggcgcaga uacaagcaua ggaauaaucg      120

gacuccugcu cacaacagcg auggcagcag agaucacacg aaggggguca gccuacuaca      180

uguaccucga cagguccgac gcaggaaaag caauaucauu cgcaaccaca cuaggaguca      240

acaaaugcca cguacaaauc auggaccuag gacacaugug cgacgccaca augucguacg      300

aaugcccgau gcuggacgaa ggaguggaac cggacgacgu agacugcugg ugcaacacaa      360

caaguacgug gguggucuac ggaacgugcc accacaagaa aggagaagcc cgacgaagca      420

gacgagcagu aacacugccc agccauucaa caagaaaauu gcaaaccaga ucccagacau      480

ggcuggaaag cagggaauac accaaacacc ucauaaaggu agaaaacugg auauuccgaa      540

aucccggauu cgcacucgua gccguagcaa uagccugguu acugggcuca agcacaagcc      600

aaaaaguaau auaccuagug augauacucc ugaucgcccc cgcguacucg auccgaugca      660

uaggagucag caaccgggac uuuguugaag ggaugucagg aggaacaugg guagacgucg      720

ugcucgaaca cggaggcugu gugacaguaa uggcgcaaga caaacccacg gucgacauag      780

agcuaguaac aacaacugua agcaacaugg cagaagugag aagcuacugc uacgaagccu      840

cgaucucaga cauggcaagc gauagccgau gcccuacgca aggagaagcc uaccuggaca      900

aacagucaga cacccaguac gucugcaaac ggacacuggu cgaccgcggc uggggcaacg      960

gcugcggccu cuucgggaag ggaucacucg ugaccugcgc aaaguucacg ugcagcaaga     1020

aaaugaccgg caaaagcaua caaccagaaa accuagaaua ccgaaucaug cucucaguac     1080

acggcucgca acacucagga augaucggau acgaaacaga cgaagaccga gcaaaaguag     1140

aagucacucc aaacagcccg cgcgcagaag caacacuagg gggcuucggg agccucgggc     1200

uagacuguga accacgcacg ggacuggacu ucagcgaccu cuauuaccua acgaugaaca     1260

acaaacacug gcugguccac aaagaauggu uccacgacau cccacuuccg uggcacgccg     1320

gagccgacac cggcacaccc cacuggaaca acaaagaagc ccuagucgag uucaaggacg     1380

cccacgcuaa aagacaaacc gucgucgucc ucggcucaca agaaggagcg guccacaccg     1440

cgcucgcggg cgcucucgaa gcggagaugg acggugccaa aggacgcuua uucuccgggc     1500

accuaaagug caggcuaaaa auggacaaau uaagacucaa aggaguaucc uacucgcucu     1560

guaccgcagc cuuuaccuuc acaaaggucc cagccgaaac ccuacacggg acagugacag     1620

uugaagucca guacgccggc acagacggac caugcaaaau cccgguacag auggcgguag     1680

acaugcaaac auugacacca gugggacgcc ucauaacugc uaacccagua auaacagaau     1740

caaccgaaaa caguaaaaug augcuggagc uggacccgcc auucggagac agcuacaucg     1800

ugauaggagu aggagacaag aaaauaacac accacuggca caggagcggc agcaccaucg     1860

gaaaagcauu ugaagcgaca gucaggggcg cgaaaagaau ggcaguacua ggggacaccg     1920

caugggacuu ugggagcgug ggaggaguuu uuaacagcuu aggaaaaggc auccaccaga     1980

ucuucggagc cgcauucaag agcuuguucg gaggcaugag cugguucagc caaauacuaa     2040

uagggacccu ccuggucugg cuugggcuga acaccaaaaa cggaagcauc agccugacau     2100

gccucgcacu aggaggcgua augauauucc uaagcacagc cgugagcgcg gacguggggu     2160

gcagcgugga cuucagcaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  7263
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 2 opt

<400>  7263
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucauaaacgc acggaaagaa aagaagagaa gaggagcaga caccagcgua gggaucgugg      120

gccugcuccu cacaacggcc auggccgccg aagucacccg cagaggauca gcuuacuaca      180

uguaccuaga ccgcaaugac gcgggcgaag caaucagcuu ccccacgacc cuaggcauga      240

acaaaugcua cauacaaaua auggacuuag gccacaugug cgacgcaacc augaguuacg      300

aaugcccgau gcuggacgaa ggggucgaac cagacgacgu agacuguugg uguaacacca      360

caucaacaug ggugguguac gggaccugcc accacaagaa aggcgaagcc aggcgaucga      420

gacgagcugu aacccuacca agccacagca ccagaaaacu acaaacgaga ucacaaacgu      480

ggcuggaguc acgcgaauac accaaacacc ucauccgagu ugagaacugg auauuccgaa      540

acccggguuu cgcauuggca gcggccgcca ucgccugguu acucggaagc ucuacgagcc      600

aaaaggucau cuaccuagua augauccucc ucauagcacc agcauacagc auccgcugua      660

uuggcgucag caacagggac uucguagaag gcaugagcgg gggcacaugg gucgacguag      720

uacuugaaca cggcggcugc gucaccguaa uggcacaaga caaacccaca gucgacaucg      780

aacucguaac aacgaccgua agcaacaugg cggaagugcg aagcuacugc uacgaagcau      840

caaucagcga cauggcgagc gacagcagau gcccuaccca gggcgaagca uaccucgaca      900

aacaaagcga cacacaauac guaugcaaga gaacucuagu cgaccgaggg uggggcaacg      960

gaugcggacu auucggaaaa ggcagccucg ucacaugugc aaaauucgcc ugcucaaaga     1020

aaaugacagg uaaaagcaua caacccgaaa accuggagua ccgcauaaug cuaagcguac     1080

acggguccca acacagcggc augauaguca acgacaccgg acacgagaca gacgaaaacc     1140

gagccaaagu ggaaauaacc ccaaacagcc caagggcaga ggccacccua ggaggguucg     1200

gcagccucgg ccucgacugc gaaccacgca ccggccuaga cuucucagac cuauacuacc     1260

ucacaaugaa caacaagcac uggcucguac acaaagaaug guuccacgac aucccccuuc     1320

cauggcacgc gggagccgac accggaacgc cccacuggaa caacaaggaa gcgcucgugg     1380

aguucaaaga cgcccacgca aaacgacaaa ccgugguagu ccuaggaagc caagaagggg     1440

caguacacac cgcacucgcc ggagcacuag aagcggaaau ggacggagcg aaagggagac     1500

ucagcagcgg acaccugaaa ugucgacuca aaauggacaa gcuacggcug aagggaguga     1560

gcuauucccu cugcacagca gccuucacau ucacaaaaau cccggccgaa acacuacacg     1620

gaaccgucac uguggaggua caauacgccg gcacagacgg ccccugcaag guaccagccc     1680

aaauggcagu agacaugcaa acacucacac cagugggccg ccuaauaacg gcaaauccag     1740

uuauaaccga gagcacggaa aacuccaaga ugaugcucga acucgauccc ccguucggag     1800

acucauacau aguaauaggc guaggggaga aaaagaucac ccaccacugg cacagauccg     1860

gcucgacaau aggaaaagcc uucgaagcca ccguacgcgg agcaaaaaga auggcgguac     1920

uaggagacac agcaugggac uucggauccg ugggcggagc ccuaaacucc cucggaaagg     1980

gaauacacca gauauucgga gcggcauuca aaucccuauu cggaggaaug agcugguuca     2040

gccagauccu cauaggaaca cuacuaaugu ggcuaggucu caacaccaaa aacggaagca     2100

uuagccucau gugccucgca cuaggcggug uacuaauuuu ccuaagcaca gccguaucag     2160

cggacguagg augcagcgua gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7264
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 2 opt

<400>  7264
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucauaaacgc caggaaagaa cgaaaacgga gaggagcaga cacgagcauu ggcaucaucg      120

ggcuacuacu aacgaccgcg auggcggcgg agaucacacg aagaggaagc gcauacuaca      180

uguaccugga cagguccgac gccggaaaag ccauuucauu ugccacaaca cuagggguaa      240

auaagugcca cguacaaaua auggaucuug gacacaugug cgacgcaaca auguccuacg      300

aaugcccaau gcuggaugag ggcguagaac cagacgacgu cgacugcugg ugcaacacga      360

caucgacgug ggucguauac ggaacaugcc accacaagaa gggggaagca cgaagaucgc      420

ggagagcagu aacacucccg agucacucga cacggaagcu acaaacaagg agccaaacau      480

ggcuggaguc cagagaauac acaaagcacc uaauaaaagu cgaaaacugg aucuuccgca      540

accccgguuu ugcccuaguc gcagucgcaa uagccuggcu acuaggcuca agcaccagcc      600

agaaaguaau auaucuagug augauacucc ugauagcgcc agcguacagc auuaggugca      660

uaggaguaag caaccgcgac uucguagaag gcaugucggg cgggaccugg gucgacgucg      720

uauuagaaca cggaggcugc gucaccguaa uggcacagga caaaccaaca guagacaucg      780

aacucguaac caccacaguc agcaacaugg cggaggucag aagcuacugu uacgaggcau      840

cuauaaguga cauggccagu gacagccgcu guccaacaca aggagaagca uaccuagaca      900

aacaaucaga cacacaauac gucugcaaga gaacccuagu agaccgggga ugggggaacg      960

ggugcggacu uuucggcaag ggcucgcucg uaacaugcgc aaaauucacg ugcucgaaga     1020

aaaugacggg caaaagcauc caaccagaaa accucgaaua caggauaaug cuaucggugc     1080

acggcucaca acacucagga augauaguaa acgacaccgg auaugaaaca gacgaaaacc     1140

gggcaaaggu agagguaaca ccgaacagcc cccgggcaga ggcgacacua ggcggcuucg     1200

gaagccuagg cuuagacugc gaaccaagaa caggccucga cuucucagac cucuacuacc     1260

uaaccaugaa caacaaacac uggcucgucc acaaagagug guuccacgac auaccgcucc     1320

cauggcacgc gggagccgac acaggaacac cgcacuggaa caacaaagag gcccuaguag     1380

aguucaaaga cgcacacgca aaaagacaaa caguaguagu gcuaggcagc caagaaggcg     1440

ccgugcacac agcccucgcc ggagcgcugg aagccgaaau ggacggcgcg aaaggcaaac     1500

uguucuccgg acaccuaaag ugccggcuca aaauggacaa gcugcgacuc aagggcguaa     1560

gcuacucccu guguacagca gccuucacau ucacaaaagu gccggccgag acccuacacg     1620

ggaccgugac aguagaagug caauacgccg gaacagacgg ccccugcaag aucccgguac     1680

aaauggcagu cgauaugcaa acacuaacac ccgucgggag auugauaacc gcaaaccccg     1740

uaauaacaga aaguacagag aacaguaaaa ugaugcuaga acuggacccg cccuucggcg     1800

acagcuacau agucaucggc gucggagaua agaaaaucac acaccacugg caccgaagcg     1860

ggagcacaau agggaaagcg uucgaggcaa cagucagggg agcgaaacgg auggccguac     1920

uaggcgauac cgcgugggac uucggaagug ucggcggcgu auucaacuca cuagggaaag     1980

gaauccacca aaucuucgga gcagccuuca agagccuauu cggcggcaug aguugguucu     2040

cacaaauacu gauaggcaca cugcugguau ggcuagggcu aaauaccaaa aacgggagca     2100

uaagccuaac cugccucgca cucgguggcg ugaugaucuu ccugagcaca gccguaagcg     2160

cggacguagg gugcagcgug gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7265
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 2 opt

<400>  7265
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc caggaaagag cgaaaacguc guggagcaga cacaucgaua ggcauagucg      120

gccuacugcu cacaaccgca auggccgcag agauaacacg cagaggcuca gcauacuaca      180

uguaccucga ccgaagugac gccggaaaag caaucucauu cgcaacaaca cuaggcguaa      240

acaaaugcca cguacaaauu auggaccuag gucacaugug cgacgccacc augucauacg      300

agugcccgau gcuagacgaa ggcguggaac cagacgacgu agacugcugg ugcaacacaa      360

cgagcaccug ggucguauac gggacgugcc accacaagaa aggcgaagca agacgaagcc      420

ggcgcgccgu gacacuccca agccacagca cccgaaaacu acagaccaga agccagacuu      480

ggcuagaaag ccgagaauac accaaacacc ucaucaaagu agaaaacugg aucuucagaa      540

acccaggguu cacgcucgua gccguagcca uagccuggcu cuuaggcuca agcacgucgc      600

aaaaagucau cuaccuagug augauacucc ugauagcgcc cgccuacagc auacgaugca      660

ucggagucag caaccgcgac uucguggagg gcaugucagg agggaccugg gucgacguag      720

uccuggaaca cggaggcugu guaacaguaa uggcccagga caaaccaaca gucgacaucg      780

aacucguaac caccaccgug uccaacaugg cagaagucag aucuuacugc uacgaagcca      840

gcaucucaga cauggccuca gacucacgcu gucccacaca aggagaagcg uacuuagaca      900

aacaaucaga cacccaauac guaugcaaac gcacgcucgu cgaucgaggu uggggaaacg      960

gcugcggacu auucggcaaa ggcucgcucg uaaccugugc aaaauucaca ugcagcaaga     1020

aaaugacagg caaaagcauc caaccggaaa accuagaaua ccggaucaug cucucgguac     1080

acggcuccca acacagcgga augauaguca acgacgagaa ccgcgcaaaa guagaaguca     1140

ccccaaacuc uccccgcgca gaagcaaccc uaggaggguu cggaucccua ggcuuggacu     1200

gcgaacccag aacaggacuc gacuucagcg accucuacua ccuaacaaug aacaacaagc     1260

acugguuggu ucacaaagaa ugguuccacg acaucccucu cccauggcau gcgggggcag     1320

acacagggac gccucacugg aacaacaaag aagcccuagu cgaguucaaa gacgcgcacg     1380

caaaacgcca aacggucgua guacucggca gccaagaagg cgcgguccac accgcgcuag     1440

ccggcgcucu cgaagcggaa auggacggag caaaaggacg ccuauucucc ggccaccuca     1500

agugccgacu aaagauggac aaacuacgau ugaaaggggu auccuauucc cucugcacag     1560

cagcauucac cuucaccaag guccccgccg aaacccuaca cggcaccguc accgucgaag     1620

ugcaauacgc aggaaccgac ggcccgugca aaguccccgc acaaauggcc gucgauaugc     1680

agacacuaac accgguagga cgacugauca cugcgaaccc cgugaucacc gaaagcaccg     1740

aaaacucaaa aaugaugcuc gaacuagacc cuccguucgg cgacagcuac aucgucaucg     1800

gggucggcga caagaaaaua acccaccacu ggcaccgcag cggaucaacu aucggcaaag     1860

cguucgaggc caccguaaga ggcgccaaac ggauggcagu acuaggcgau acagcauggg     1920

auuucggguc cguaggaggc gucuucaaca gccucggaaa aggcauccac caaauauucg     1980

gcgccgcguu caagagccua uuuggcggaa ugagcugguu cucacaaauc cugaucggaa     2040

cucuccuagu cuggcuagga cuaaacacga aaaauggauc aauaagccua acaugccugg     2100

cacuaggagg cgucaugaua uuccuaagua cagcagucag cgccgacguc ggaugcagcg     2160

uagacuucag caaaugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7266
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 2 opt

<400>  7266
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucauaaacgc gagaaaagaa cgaaaacgcc gcggggcgga cacgagcauc ggcauaaucg      120

gacuguugcu aacgaccgcg auggccgcgg agaucacaag acgaggcagc gcguacuaua      180

uguaccucga ccgguccgac gccggaaaag ccaucucguu cgccaccacc cuaggaguca      240

acaaaugcca cguccaaaua auggaccucg gccacaugug cgaugccaca augucauacg      300

agugccccau gcuagacgaa gguguagaac cagacgacgu agacugcugg ugcaauacca      360

cgagcacgug gguaguuuac ggcacguguc acagaaagaa aggagaagcc aggagaucaa      420

gacgcgcggu cacccuccca agccacucaa cacgcaaauu acagaccaga agccagaccu      480

ggcucgaguc cagagaauac accaaacacc ugauaaaagu cgaaaacugg aucuuucgca      540

accccggauu cgcacucgug gcaguagcga uagcauggcu ccuaggcagc agcacauccc      600

aaaaagugau uuaucucguc augauccuac ugauagcacc ggcguacagc aucaggugca      660

uaggaguaag uaacagggau uuuguagaag gcaugucugg cggaacgugg guagacgucg      720

uacucgagca cggaggcugu gucacaguca uggcccaaga caaacccaca gucgacaucg      780

agcucgucac cacgacaguc agcaacaugg cagaagugcg aucauacugc uacgaagcgu      840

cgaucagcga cauggcaucc gacucgcgcu gccccacgca aggugaagca uaccuagaca      900

agcaaucaga cacacaauau gucugcaaac gcacccucgu cgaccgagga uggggcaacg      960

gaugcggacu cuuuggaaaa ggaucccuag uaaccugcgc caaauuuacc ugcagcaaaa     1020

agaugacggg caagagcauc caacccgaaa accucgagua cagaaucaug cuguccgucc     1080

acggcucgca acacucagga augauaguca acgacacagg cuacgaaaca gaugaaaaca     1140

gggcaaaagu cgaagucacc cccaacuccc cacgcgcgga agccacccug ggcggauucg     1200

gcucccuagg ccuagacugc gaaccacgca caggacucga cuucagcgac cuguacuacc     1260

uaacaaugaa caacaaacac uggcuagucc acaaggagug guuccacgac auaccacucc     1320

ccuggcacgc aggcgccgac accggaaccc cgcacuggaa caacaaagaa gcacucgucg     1380

aguucaaaga cgcacacgca aagagacaaa cgguaguagu ccugggaucc caagaaggcg     1440

caguacacac agcacuggcc ggcgcccuag aagccgaaau ggacggcgcg aaaggaaaac     1500

uguucagcgg ccaccucaaa ugccgccuaa aaauggacaa acuacgacuc aaaggaguau     1560

cuuacucccu auguaccgca gccuucaccu ucaccaaagu accggccgaa acgcuccacg     1620

gaacugugac agucgaggua caauacgccg gcaccgacgg acccugcaaa auaccaguac     1680

aaauggccgu agacaugcaa acgcuaacgc ccguaggacg gcuaaucaca gcgaaccccg     1740

ugauaacaga aucaaccgag aacucaaaaa ugaugcucga acuagacccg cccuucggcg     1800

acagcuauau agugauaggc guaggcgaca agaaaauaac ccaccacugg cacagaagcg     1860

gauccaccau cggcaaagcg uucgaagcaa ccguaagggg cgccaaaaga auggcagucc     1920

uaggagauac cgccugggac uucggaagcg ucggaggagu cuucaacagc cuuggaaaag     1980

ggauucacca aaucuucgga gccgccuuca aaagccuauu cgggggcaug uccugguuca     2040

gccaaauacu aaucggcacc cuauuagucu ggcucggccu caauaccaaa aauggcagca     2100

uuagccuaac cugccuagca cucggaggcg ucaugaucuu ccugagcaca gccguaagcg     2160

ccgacguagg cugcuccgua gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7267
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 2 opt

<400>  7267
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaacgc cagaaaagaa cggaaacgac gaggcgcgga cacaucaaua ggaaucauag      120

gacugcugcu gacaacagcg auggcggccg agauaacaag acgaggcucg gccuacuaca      180

uguaccucga ccggagcgac gcagggaaag cgauaucauu cgcaacgaca cucggaguaa      240

acaagugcca cguacaaauc auggaccucg gacacaugug cgacgccacg augucauacg      300

agugccccau gcuggacgag ggcgucgaac cagacgacgu agacugcugg ugcaacacca      360

caagcacaug gguaguauac ggaaccugcc accacaagaa aggcgaagcg cgaaggucaa      420

gacgagcagu cacccuacca agccacagca caagaaaacu ccaaacccgc ucacaaaccu      480

ggcuagaaag ccgagaauau accaaacacc ucaucaaagu agaaaacugg auauuccgca      540

acccaggcuu cgcacucgug gcaguagcca uagcaugguu auuaggcagu agcacuagcc      600

aaaagguaau cuaccuaguc augauacucc uaaucgcgcc cgcguacuca aucagaugca      660

ucggaguaag caacagagac uucguagaag ggaugucagg gggaacaugg guagacgucg      720

uacuagaaca cggaggaugc gucacgguaa uggcccagga caagccaacc gucgacauag      780

agcucgugac cacuaccgua agcaacaugg cggaaguacg aucauauugc uacgaagccu      840

caauaucaga cauggccucu gacagccgcu gcccaacaca aggagaagcg uaccuagaca      900

agcaaagcga cacgcaauac gucugcaaac ggacacucgu agaccgcgga uggggaaacg      960

gcugcggucu auucggaaag ggcucgcucg uaaccugcgc aaaauuuacc ugcucaaaga     1020

aaaugacagg caaaucaauu cagccggaaa accucgagua caggaucaug cucuccguac     1080

acggcucaca acauagcggc augacaguca acgacauagg auacgaaacu gacgaaaaca     1140

gggccaaagu cgaaguaacg cccaacagcc caagagcaga agccacacug ggaggauucg     1200

gcagcuuagg ccuggacugc gaaccgcgaa ccggauugga cuucucagac cucuacuacc     1260

uaaccaugaa caacaaacac uggcuagucc acaaagaaug guuccacgac auaccacuac     1320

cguggcacgc aggcgcagac accgggaccc cgcacuggaa caacaaagaa gcgcuggucg     1380

aguucaaaga cgcgcaugcc aaacgacaaa ccgucguggu cuuaggaucc caagaaggcg     1440

cgguccacac ggcacuggca ggcgcucucg aagccgaaau ggacggagca aaaggaaagc     1500

uauucagcgg ccaccucaaa ugcagacuca aaauggauaa acugaggcuc aaagggguau     1560

cguacucccu gugcacagca gccuucaccu ucacaaaagu ccccgcagag acccuccacg     1620

gcacagugac aguggaaguc caauacgccg ggacagacgg accaugcaaa aucccagucc     1680

agauggcagu agauaugcaa acacugacac ccguaggaag acuaaucacg gcaaaccccg     1740

ugauaacaga aucgacagaa aacuccaaaa ugaugcugga auuagaccca cccuucggcg     1800

auagcuacau cguaauagga gucggggaca agaaaauaac acaccacugg caccgcagcg     1860

gaagcaccau cggcaaagca uucgaggcga cggucagagg cgcaaaacga auggcggucu     1920

uaggagacac agcaugggac uucggguccg ugggaggggu guucaacuca cuaggaaaag     1980

gaauccauca aaucuucgga gccgcauuca aaagccuauu cggagggaug uccugguucu     2040

cacaaauacu aauaggcaca cugcugguau ggcuaggacu caacaccaaa aacgggagca     2100

uaagccuaac cugccucgca cuagggggag uuaugaucuu cuugagcacg gccgucagcg     2160

cagacgucgg cugcucagua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7268
<211>  2512
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 2 opt

<400>  7268
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaacgc ccgcaaagaa cgcaaacgaa gaggcgcaga cacaagcaua ggcaucaucg      120

gccuccuccu cacaaccgcc auggcagcag aaauaacccg cagaggaagc gccuacuaca      180

uguaccuaga ccgcuccgac gcaggcaaag ccauaucauu cgcaacaacc cugggaguaa      240

acaaaugcca cguccaaaua auggaccugg gccacaugug cgacgccacg auguccuacg      300

aaugcccaau guuagacgaa ggaguagaac ccgacgacgu cgacugcugg uguaacacaa      360

cuagcacaug gguagucuau ggaaccugcc accacaagaa aggagaagca agacgauccc      420

gccgcgcggu gacccugcca agccacucaa cacgaaaacu acaaaccaga ucccaaaccu      480

ggcucgaauc acgugaauac accaaacacc ucauaaaggu cgagaauugg auauuccgaa      540

acccaggcuu cgcccuagua gcaguagcaa uagcauggcu gcucgggagu agcaccagcc      600

aaaaggucau cuaccuaguc augauacuac ucauagcccc agcauacagc auccgaugca      660

uaggaguauc caaccgcgac uucguagaag ggaugagcgg aggcaccugg guagacguag      720

uccucgaaca uggaggaugc guaacgguaa uggcacagga caagcccacg gucgacauag      780

aauuagugac gaccacagua uccaacaugg ccgagguacg aaguuacugc uacgaagcgu      840

caauauccga cauggcgagu gacucgcgcu gccccacaca aggggaggcu uaccucgaca      900

aacagagcga cacacaauac gucugcaaac gaacccuagu cgaccgcgga ugggguaacg      960

gcugcggacu auuuggcaaa ggcagccuag ugaccugcgc caaauucaca uguuccaaaa     1020

agaugacugg aaaauccaua caaccagaaa accucgaaua ccgcaucaug cuauccguac     1080

acggcucaca acauucaggc augauagggu acgaaacaga cgaaaaccgc gccaaaguag     1140

aagucacacc aaacagccca agagcagagg ccacacuagg aggcuucgga ucccucggac     1200

ucgacugcga gccgcgaacc ggccucgacu ucucggaccu auacuaccuc acaaugaaca     1260

acaaacacug gcucguccac aaagaauggu uccacgacau cccccugcca uggcacgcag     1320

gcgcagacac cggaacaccg cacuggaaca acaaagaagc acugguagag uucaaagacg     1380

cccaugccaa acgccaaaca guaguaguuc ugggauccca agaaggcgca guccacacug     1440

cccucgcggg cgcccucgaa gcagaaaugg acggcgcuaa aggcaaacua uucuccgggc     1500

accucaaaug ucgacuaaaa auggacaaac uccgacuaaa aggcguaagc uacucucucu     1560

gcaccgccgc guucacguuc acaaaagucc ccgccgaaac acuccacggc acagucaccg     1620

ucgaagucca auacgccgga accgacggac ccugcaaaau acccgugcag auggcaguag     1680

acaugcaaac cuuaacacca gucggacgcc ucauaaccgc aaacccaguc aucacagaaa     1740

gcaccgaaaa cuccaaaaug augcuagaac ucgacccgcc cuucggcgac uccuacaucg     1800

ugauaggcgu gggggacaag aaaaucacac accacuggca ccgaagcggc agcacaaucg     1860

gaaaagccuu cgaggccaca gucagaggcg caaaaagaau ggccgugcua ggagacaccg     1920

caugggacuu cgguuccgua ggcggcguau ucaacagccu cggcaaggga auccaccaaa     1980

ucuucggcgc ggcuuucaaa agccucuucg gcggcauguc cugguucuca cagauccuga     2040

ucggcacacu ccucgucugg cuaggccuaa acaccaaaaa cggcucgaua aguuuaacau     2100

gccucgcacu gggcggaguc augaucuucc ucucaaccgc aguuagcgcc gacguaggau     2160

gcagcguaga uuucuccaaa ugaggacuag ugcaucacau uuaaaagcau cucagccuac     2220

caugagaaua agagaaagaa aaugaagauc aauagcuuau ucaucucuuu uucuuuuucg     2280

uugguguaaa gccaacaccc ugucuaaaaa acauaaauuu cuuuaaucau uuugccucuu     2340

uucucugugc uucaauuaau aaaaaaugga aagaaccuag aucuaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2460

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2512


<210>  7269
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 2 opt

<400>  7269
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc acgaaaggaa aagaaacgca gaggagcaga cacaagcguc ggcauaguag      120

gacuccuacu aacaacagcc auggccgcag aagucacaag aaggggaagc gcauauuaca      180

uguaccucga ccgcaacgac gcuggcgaag ccaucagcuu cccgaccacc cuaggcauga      240

acaaaugcua uauacaaaua auggaccucg gccauaugug cgacgcaaca augagcuacg      300

aaugccccau gcucgacgaa ggcgucgaac cagacgacgu cgacugcugg ugcaacacaa      360

ccucaacaug ggucgucuac ggcacgugcc accacaaaaa gggagaagcc agacgcuccc      420

gacgagccgu uacccucccu agccacagca cgagaaaacu ccaaacacga ucacaaaccu      480

ggcuugaaag ccgcgaauac accaaacacc ucauccgcgu ggaaaacugg auauuccgaa      540

aucccggcuu cgcccucgca gcagccgcga ucgcauggcu ccuuggcucc uccacguccc      600

agaaagucau cuaucucguc augauccuac uuaucgcgcc ugcauacucc auaagaugca      660

ucggcguaag caacagagac uucgucgaag gcauguccgg cggcaccugg gucgacgucg      720

uccuagaaca cggggguugc guaaccguca uggcucagga caaaccaaca gucgacaucg      780

aacugguaac aacgaccguc agcaacaugg ccgaaguacg cagcuacugc uaugaggcuu      840

caauaagcga cauggccagc gacagccggu gcccaacaca aggagaagcc uaccuagaca      900

aacaaucaga cacccaauac gucuguaaga gaacccuggu ggaccgcgga uggggaaacg      960

gcugcggauu guucggcaag ggcucccuag uaacaugcgc aaaauucgcc ugcuccaaga     1020

aaaugaccgg caaaucgauc caacccgaaa accucgaaua ccgcauaaug cuauccgucc     1080

acggcucaca acacucaggc augauaguaa acgacaccgg acacgaaaca gacgaaaaua     1140

gagcgaaagu ggagaucacu cccaacagcc caagagcgga agccacacua ggaggcuucg     1200

gcucccuagg acucgacugu gaaccacgaa ccggacucga uuucucggac cucuacuacc     1260

uaacaaugaa caacaaacac uggcucgugc acaaagaaug guuccacgac auaccccucc     1320

cuuggcacgc aggagcagac accggcaccc cacacuggaa caacaaagaa gcccucguag     1380

aguucaaaga cgcccacgcc aaaagacaaa caguuguagu acugggcagc caagaaggag     1440

ccgugcacac cgcccuagcc ggagcccuag aagccgaaau ggacggggcu aaaggccguu     1500

uauccagcgg ccaccuaaaa ugcagacuaa aaauggacaa acuaagguua aaaggcguaa     1560

gcuacucccu augcacggcg gcauucaccu ucacaaaaau ccccgcagaa acccuccacg     1620

gaacagucac aguggaaguu caauacgccg gcacagacgg acccuguaag guaccagcac     1680

aaauggcugu cgacaugcag acucugacac caguugggcg acucaucacc gcaaaccccg     1740

ucaucacuga aagcaccgaa aacuccaaga ugaugcuaga acuugaccca ccauucggag     1800

acagcuacau aguaauuggc gucggcgaaa agaaaaucac acaccacugg cacagaagcg     1860

gcagcacgau agguaaagca uucgaagcaa ccguaagggg cgccaaacga auggcagugc     1920

ucggagacac cgccugggac uucggaagcg uaggaggcgc ccugaacuca cuaggccgag     1980

gcauacauca aauauucggg gcagcauuca aaagccucuu cggcggcaug agcugguuca     2040

gucaaauacu aauagggaca cugcuaaugu ggcuaggacu gaacaccaaa aacggcucaa     2100

uaucacuaau gugccuagca cucggaggcg ugcuaauauu ccuaagcaca gcagucucgg     2160

cagacguagg augcagcgua gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7270
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 2 opt

<400>  7270
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc aaggaaggag aaaaagcgac gaggcgcaga cgcgagcguc ggcauaguag      120

gccuccugcu cacaaccgcg auggccgccg aggucacacg cagaggcucg gccuauuaca      180

uguaccucga cagaaaugac gcaggcgaag ccaucucauu cccgaccaca cucggaauga      240

acaaauguua cauacaaauc auggaccuag ggcacaugug cgacgcaacu auguccuacg      300

aaugcccgau gcucgacgaa ggaguagaac cagacgacgu agauugcugg ugcaacacaa      360

caagcacaug gguagucuau ggcacgugcc accacaaaaa gggggaggcg aggaggagca      420

gaagggcagu uacacuccca agccacagca cgcgcaaacu acaaacaagg agccagaccu      480

ggcuugaaag cagagaauac acaaagcacc ugaucagggu cgaaaacugg auauuccgca      540

acccggguuu cgcacuagca gcagcagcaa ucgcauggcu ccuaggaagc agcacguccc      600

aaaaaguaau auaccucgua augauacugc ucaucgcccc agcuuauuca aucaggugca      660

ucggcgugag caacagagac uuuguggaag gaaugucagg cggcacgugg gucgacgucg      720

uucuagaaca cggaggaugc gucacaguaa uggcucagga caaaccaaca gucgacauag      780

agcuaguaac aacaaccgua agcaacaugg cagaaguucg caguuacugc uaugaagccu      840

cgaucucaga cauggccuca gacucgagau gccccacaca aggagaagcg uaccuagaca      900

aacaaucaga cacccaauac guaugcaaaa ggacccuagu agaccgcgga ugggggaacg      960

ggugcggacu auucggaaag ggaucgcucg uaaccugcgc aaaauucgcc ugcagcaaaa     1020

agaugacugg caaaagcaua caacccgaaa accuggaaua cagaaucaug cuaucagucc     1080

acgggagcca acacucaggc augauaguaa acgacacggg ccacgaaaca gacgaaaaca     1140

gagcuaaagu agagauaaca cccaacagcc cacgagcgga agcgacacua ggagguuucg     1200

gaagccuagg ccuggacugc gaaccgcgaa caggccucga uuucagcgau cucuacuacc     1260

ugaccaugaa uaacaaacac uggcucguac auaaagagug guuccacgac auaccgcuac     1320

cauggcaugc cggagcagac accggaacac cccacuggaa caacaaagag gcccuagucg     1380

aguucaaaga cgcgcacgcc aaaagacaaa cagugguggu acugggaucg caggagggcg     1440

cgguccacac ugcacucgcc ggcgcgcuag aagccgagau ggacggagca aaaggacgcc     1500

uaagcagcgg acaccucaag ugccgacuaa agauggacaa gcuccgccua aaaggggucu     1560

cguacagucu cugcacggca gcauucaccu ucacgaagau cccggcggaa acacugcacg     1620

gaaccguaac aguagaagug caauacgccg ggaccgacgg cccgugcaaa gucccggccc     1680

aaauggcagu agacaugcaa acgcuaacac caguaggaag acugaucacu gccaacccag     1740

uaaucacaga aucgacagag aacucaaaaa ugaugcuaga auuggacccg cccuuuggcg     1800

acagcuacau cgugauagga guaggggaaa agaaaauaac acaccacugg caccgaagcg     1860

gaagcacaau cgggaaagcc uucgaggcaa caguccgagg cgcaaagaga auggcagucc     1920

uuggagacac ggcaugggac uucggaagcg ucgggggcgc cuugaacuca cuaggcaaag     1980

ggauccacca aauauucgga gccgcguuca agagccucuu cggcggcaug uccugguuuu     2040

cacaaauacu caucggaaca uuacugaugu ggcucgggcu uaacaccaag aacggcucaa     2100

uaagccugau gugccucgcc cuagggggcg uacucauauu ccuguccacg gccgucagcg     2160

ccgacguagg augcagcgua gauuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7271
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 2 opt

<400>  7271
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucaucaaugc acggaaagag aaaaagcgcc gaggggccga cacgagcguc gggaucguag      120

gccuacugcu gaccaccgca auggccgcag aagucacacg acgaggcagc gcauacuaca      180

uguaccucga cagaaacgac gccggagaag ccaucucauu ccccacaaca cucgggauga      240

acaaaugcua cauccaaaua auggaccuag gacacaugug cgaugcaacc augagcuacg      300

aaugcccaau gcucgaugaa ggcguugaac cagacgacgu ggacugcugg ugcaacacaa      360

cuagcaccug ggucgucuac ggcacaugcc accacaagaa aggggaggca cgcagaaguc      420

gacgggccgu aaccuuaccc agccacucca caagaaaacu acaaacacgc ucacaaaccu      480

ggcuggaaag ucgagaauac accaaacacc ucauccgagu ggaaaacugg aucuucagaa      540

acccgggacu agcacuagcc gccgcagcaa ucgcauggcu acuaggaagc uccacaagcc      600

aaaaagucau auaccucguc augauccuac ugaucgcccc agcauacagu auacgcugca      660

uaggagugag caacagagac uucguagaag gcaugucagg aggaacgugg gucgacguag      720

uccucgagca cggcggcugc guaaccguaa uggcacaaga caaacccacc guagacauag      780

aacucgucac cacaaccguu agcaacaugg cagaggucag gucuuacugc uacgaagcaa      840

gcauaagcga cauggcaucc gacucaagau gccccaccca aggcgaagca uaccuagaca      900

aacaauccga cacacaauac guaugcaaac gaacccuagu agaccggggc uggggcaacg      960

gcugcggacu auucggcaaa gggagccuag ucacaugcgc aaaauucgca uguuccaaga     1020

aaaugaccgg caaauccauc caaccagaga accucgagua ccgcauaaug cugucaguac     1080

acggcuccca acacuccgga augauaguga acgacaccgg ccacgaaacc gacgagaaca     1140

gagccaaagu cgaaauaaca cccaacucac cccgggcaga ggcaacccuc ggaggcuucg     1200

gcagucucgg acucgacugc gaaccccgua ccggucucga cuuuucagac cuauacuacc     1260

uuacuaugaa caacaaacac uggcuagugc acaaagagug guuccacgau aucccccuac     1320

cauggcacgc cggggccgac accggcacac cacacuggaa caacaaagaa gcgcucgucg     1380

aguucaaaga cgcgcacgca aagcgccaaa ccgugguagu ccuaggcagc caagaagggg     1440

caguccacac agcacuagcc ggagcgcugg aagcagaaau ggacggagcc aaaggccgcc     1500

uauccagcgg acaucuaaaa ugcagacuaa aaauggacaa acuccgucuc aaaggaguau     1560

ccuacagccu cugcacagca gcuuucaccu ucacgaagau ccccgccgaa acccuccacg     1620

gcacagucac agucgaaguc caguaugccg gaacagacgg accaugcaaa gugccggccc     1680

aaauggccgu ggacaugcaa acacuuaccc caguaggccg gcuaaucaca gcaaacccag     1740

ugaucaccga aagcaccgaa aacuccaaaa ugauguuaga acuggaccca cccuucggcg     1800

auuccuacau cgucaucggc gugggcgaaa agaaaaucac acaccauugg caccgaagcg     1860

gcuccaccau cggaaaggca uucgaagcca ccguaagagg cgccaagcgc auggcaguac     1920

uaggagacac agcgugggac uucggcagcg ucggaggagc ucuaaacagc cugggaaagg     1980

guauacacca aaucuucgga gcggccuuca aaagccucuu cgggggcaug ucaugguuca     2040

gccagauccu aaucggaacc cuacuaaugu ggcucggccu aaacaccaag aacggaagca     2100

uaucgcugau gugcuuagca cucggcggag uacuuaucuu ccucucaaca gccguauccg     2160

ccgacgucgg cugcagcguc gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7272
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 2 opt

<400>  7272
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc acgcaaagag aagaaacgac ggggagccga caccucagua ggcaucgugg      120

gacuacuucu cacaacggca auggcagccg aaguaacacg cagaggaagc gccuacuaca      180

uguaccuaga ccgcaacgac gccggcgaag caaucagcuu ccccacaacc cuaggaauga      240

acaaaugcua cauacaaaua auggaccucg gccacaugug cgacgccaca augucauacg      300

aaugccccau gcuagacgaa ggaguagaac ccgacgacgu cgacugcugg ugcaacacga      360

caagcaccug gguuguauac ggaaccugcc accacaagaa aggcgaagca cgccgcucac      420

ggagagccgu aacccucccc ucacacagca caagaaaacu gcaaacacgg ucgcaaaccu      480

ggcuagaauc acgcgaauac accaaacacu uaauccgagu cgaaaacugg auauuccgca      540

auccaggcuu cgcacucgcc gcagccgcca ucgcauggcu acugggaagc uccacaagcc      600

aaaaaguuau cuaccucgua augauccuac uaaucgcccc cgcuuacucg auacggugca      660

uaggaguauc gaacagggac uucguagagg gcauguccgg cggcacaugg gucgacgucg      720

ucuuagagca cgggggcugc guaaccguca uggcucaaga caaaccgacc guggacaucg      780

aacucguaac cacaaccgug agcaacaugg ccgaaguaag aagcuauugu uacgaagcaa      840

gcaucucaga cauggcaagc gacagcagau gcccaaccca gggagaagca uaccuagaca      900

aacaaucaga uacacaauac gugugcaaaa ggacacucgu agacagagga ugggggaacg      960

gaugcgggcu uuucggaaaa ggaucacucg ucacaugcgc aaaauucgca ugcagcaaga     1020

aaaugacagg caaaucaauc caacccgaga accuggaaua cagaauaaug cuauccgucc     1080

acgggagcca acacuccgga augauaguaa acgacacagg acacgaaacu gacgagaacc     1140

gagccaaagu cgagaucaca ccaaacagcc cacgcgcgga agccacacuc ggcggauucg     1200

gaucccucgg ccucgacugc gagccacgaa ccggccuaga cuucucggac cuauacuacc     1260

uaaccaugaa caacaaacac uggcuggugc acaaagaaug guuccaugac auaccgcuuc     1320

cauggcacgc aggagcagac accggcacac cccacuggaa caacaaagaa gcgcuuguug     1380

aguucaaaga cgcccacgcc aaaagacaaa ccguaguagu acucggcagc caagaaggug     1440

ccguacacac agcgcuugcc ggcgcccucg aagcagaaau ggacggcgca aaaggacgcc     1500

uuagcagcgg ccaccuaaaa uguaggcuca aaauggacaa acuaagacua aaaggggucu     1560

ccuauucccu augcacggca gcauucaccu ucacgaaaau ccccgcagaa acccuccacg     1620

gaaccguaac agucgaagua caguacgcag gaaccgacgg accgugcaaa gucccagccc     1680

agauggcagu ggacaugcaa acccugacgc cagucggccg acucaucacc gccaacccug     1740

ugaucacgga gagcacagaa aacagcaaaa ugaugcucga acucgaccca cccuucggcg     1800

acucguacau agucauaggc guaggcgaaa agaaaauaac acaccacugg uaccgcuccg     1860

gcagcacaau cgggaaagca uucgaagcca cagucagagg ggcgaaacgc auggccguac     1920

uaggagacac agccugggac uucggcagcg uaggaggcgc acuaaacagc cuagggaaag     1980

gcauacacca aaucuucgga gccgccuuca aaagccuauu cgguggaaug uccugguucu     2040

cccaaauacu aauaggcaca cuccuaaugu ggcucggacu aaacaccaaa aacggaucaa     2100

ucucccuaau gugucucgcc cuaggcggag uacucauauu ucuauccaca gcagucagcg     2160

cagacguagg guguucaguc gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7273
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 2 opt

<400>  7273
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucauaaacgc ccggaaagaa aagaaacgcc gcggagccga caccuccguc ggaauaguag      120

gccugcugcu caccacggcc auggcugccg aagugacccg ccgaggaagu gccuacuaca      180

uguaccucga cagaaacgac gcaggcgaag caaucagcuu ccccacaacg uuaggcauga      240

acaaaugcua uauacagaua auggaccugg gccauaugug ugaugcaacc augucauacg      300

aaugccccau gcucgacgaa ggagucgaac cagacgacgu agauugcugg ugcaacacaa      360

ccucgacaug gguagucuau ggaacaugcc accacaagaa aggcgaagcc cgccgcucaa      420

gacgagcggu aacgcuaccu ucgcauucca cacgaaaacu acaaacccgc ucccaaacau      480

ggcuagaaag ccgagaauac acaaaacacc ucaucagggu agagaacugg aucuuccgca      540

acccaggcuu cgcacucgcc gcagcagcca ucgccuggcu acuaggaagc agcacaucgc      600

aaaaaguuau cuaccucgua augauccucc uuaucgcccc ggccuacagc auacgcugca      660

ucggggucuc caacagagac uucgucgaag gcaugagugg gggcacgugg guagacgucg      720

uacuagaaca cggcggaugc gugaccguca uggcacaaga caaacccacc guggacauag      780

agcuaguaac aaccacugua ucgaacaugg ccgagaucag aagcuacugc uacgaagccu      840

ccauauccga cauggccagc gacagcagau gcccgacaca aggagaagcc uaccucgaca      900

aacaaagcga cacacaauau gucugcaaac gcacccuagu cgacagaggc uggggcaacg      960

ggugcggccu cuucggcaaa ggaagccucg ucacaugcgc gaaauucgca ugcuccaaaa     1020

agaugaccgg aaaaucgaua caacccgaga accucgaaua ccgaaucaug cuaagugucc     1080

acggcagcca acacucagga augaucguca augacaccgg acacgagaca gacgaaaacc     1140

gagccaaagu agaaauaacc ccaaacucac cacgggcaga ggcgacgcua ggcggauucg     1200

gcucccuggg ccucgacugc gaaccacgga ccggacucga cuucagcgac cuauacuacc     1260

ucacgaugaa caacaaacac uggcuaguac acaaagaaug guuccacgac aucccacugc     1320

cauggcacgc aggcgcagau acaggcacac cccacuggaa caacaaagag gcccuaguag     1380

aguucaaaga cgcacacgcc aaaagacaaa ccguaguggu acucggcagc caagaaggcg     1440

caguacacac agcacucgcc ggcgcacugg aagcagaaau ggacggagcc aaaggccggc     1500

uauccagcgg ccaccugaaa ugccgccuca aaauggacaa gcucaggcuc aagggaguaa     1560

gcuacagccu augcaccgca gcauuuaccu ucacaaaaau ccccgcggaa acucugcacg     1620

gcaccgucac agucgagguu caauacgcag gcaccgacgg cccaugcaag guccccgcac     1680

aaauggcugu ggacaugcaa acacucaccc cugucggcag acucauaacc gccaacccag     1740

ucauaaccga gagcacagaa aauuccaaaa ugaugcuaga acuagacccu cccuucgggg     1800

acagcuacau cgucaucggc guaggggaga aaaagaucac ccaccacugg caccggucag     1860

gcucaaccau aggcaaagcc uucgaagcaa caguaagagg agccaaacgc auggccguac     1920

ugggagauac agccugggau uucggaucug ucggaggcgc acuuaacagc cuuggcaaag     1980

gcauccacca aaucuucggc gccgccuuca agucccucuu cggcggaaug agcugguuca     2040

gccaaauccu aaucggcacc cuccuaaugu ggcucggacu caacaccaaa aacgguucca     2100

ucucauuaau gugccuagcc cucggaggug uccucauauu ccuauccacc gcagucuccg     2160

ccgacguagg cuguagcguc gacuucucua agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7274
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 2 opt

<400>  7274
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc aagaaaagaa aagaaacgca ggggagcaga caccucgguc ggcauagucg      120

gacuccuacu caccaccgcc auggcugcug aaguuacacg cagaggaucg gccuacuaca      180

uguaccugga ccgcaacgac gcaggggagg ccauaucguu ccccaccaca cuuggaauga      240

acaaaugcua cauacaaauc auggaccucg ggcacaugug cgacgcaacc auguccuacg      300

aaugccccau gcucgacgaa ggaguagaac cagacgaugu cgacugcugg ugcaacacca      360

cgagcacaug ggucguuuac ggcacaugcc accacaagaa aggugaagcc cgccgcaguc      420

gacgagccgu cacccuucca agccacucca cacgaaaacu ccaaaccagg agccaaaccu      480

ggcucgaaag ccgagaauac accaaacacc ucauaagagu agagaacugg auauuccgca      540

acccaggcuu cgcccucgca gccgccgcca ucgcguggcu ccuaggcagu agcaccagcc      600

aaaaagugau auaccuaguc augauacuac ugaucgcacc agcauacuca auccggugca      660

uaggaguauc uaaccgagac uucguagaag gcaugucagg agggacaugg guggacguag      720

uccucgaaca cggagggugc guaacaguaa uggcccaaga caaacccacc gucgacaucg      780

agcuagucac cacgaccguc agcaacaugg cagaaguccg guccuacugc uacgaagcaa      840

gcaucucgga cauggccagu gacucccgcu gccccacgca gggcgaagca uaccucgaca      900

aacaauccga cacccaauac gucuguaaac gcacacucgu agaccgcggc uggggaaacg      960

gcugcgggcu auucggaaag ggcagcuuag ucacaugcgc caaguuugca ugcucaaaga     1020

aaaugaccgg caaaagcaua caacccgaaa accucgaaua ccguaucaug cuguccguac     1080

acggaucaca acacagcgga augaucguca acgacaccgg ccacgaaaca gacgaaaaca     1140

gagcaaaagu cgaaauaacu cccaauagcc cacgcgcaga ggccacacug ggcgguuucg     1200

gcagccuggg ccuagacugc gaaccccgca caggccucga cuuuuccgac cucuacuacc     1260

ucaccaugaa caacaaacac uggcucgucc acaaagaaug guuccaugac aucccccucc     1320

cauggcacgc cggcgccgac accggaaccc cacacuggaa caacaaagaa gcccuagucg     1380

aguucaaaga cgcccacgcg aaacgacaaa ccguaguagu acucggcagc caagaaggcg     1440

caguucauac ggcccucgcg ggagcacucg aagcugaaau ggacggcgca aaaggacgac     1500

ucucaucagg acaucugaaa ugccgacuca aaauggacaa auuaagacuc aaaggcguau     1560

ccuacucgcu cugcacggca gcauucacgu uuacaaaaau accagcagaa acccugcacg     1620

gcaccguuac cguagaaguc caauacgccg guaccgacgg cccgugcaag guccuagcac     1680

aaauggcagu cgacaugcaa acauugacac ccgucggccg acugaucaca gcaaaccccg     1740

ugaucaccga aucaaccgaa aacuccaaaa ugaugcucga acuggaccca ccauucggcg     1800

auagcuauau cgucaucgga gucggcgaaa agaaaaucac ccaccacugg caccgauccg     1860

gcuccacaau cggaaaagca uucgaagcaa caguacgggg cgcaaaacgg auggcagucc     1920

ucggcgacac agccugggac uucggcagcg ucggcggcgc ccucaacagc cuaggcaaag     1980

gcauccacca aauauucggu gcagccuuca agagccucuu cggaggaaug agcugguuca     2040

gccaaauacu caucggcaca cuccuaaugu ggcugggccu caacaccaaa aacggcagca     2100

uaagccucau gugucuggcc cuaggaggag uacuaauauu ccuauccacu gccgucagcg     2160

cagacguugg cugcagcgua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7275
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 2 opt

<400>  7275
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc acgaaaggag aaaaaacgcc gaggggccga uaccuccguu ggcaucgugg      120

gacuccuccu aacuacagcu auggccgcgg aagugacccg ccgcggaagc gcuuacuaca      180

uguaccuaga ucgcaacgac gccggcgaag ccaucucauu cccaacgaca cugggcauga      240

acaaaugcua cauccaaauc auggaccuag gccacaugug cgacgccacc augucuuacg      300

aaugucccac acuugacgaa ggcguagagc ccgacgacgu agacugcugg ugcaacacga      360

cgucaaccug gguuguauac ggcacaugcc accacaaaaa gggagaagcc agacgcagca      420

gaagagccgu cacacuaccc agccacucga ccagaaaacu acagacccgc agccaaacau      480

ggcuggaauc ccgcgaauac acaaaacacc uaauacgcgu agaaaacugg auauucagga      540

acccgggcuu cgcccuagcc gcagcagcaa ucgccuggcu ccuaggcucc uccacauccc      600

aaaaagucau auaucucguc augaucuuac ucaucgcccc agccuacucu auacgcugca      660

ucggcgucuc caaccgcgac uucgucgaag gcauguccgg aggaacaugg guagacguag      720

uacuggagca cgggggaugc gucacaguaa uggcccaaga caaacccacc guagacauag      780

aacuagucac caccacaguc ucaaacaugg ccgaaguccg cucauacugu uacgaagcaa      840

gcauauccga cauggccucc gacucgcggu gcccaaccca aggcgaggcc uaccuagaca      900

aacaaagcga cacccaauac gucugcaagc guacgcuggu ggaccgugga uggggaaacg      960

gaugcggacu auucggcaaa ggcagccucg uuaccugcgc aaaauucgcc ugcuccaaaa     1020

aaaugacagg caaaucgauu caacccgaga accucgaaua ccgaauaaug cuaagcgucc     1080

acggaucaca gcacuccggc augaucguca acgacaccgg acacgagaca gacgagaacc     1140

gcgcaaaagu ggaaaucacc ccgaacuccc cccgugcaga ggcaacccua ggcggcuucg     1200

gcucacucgg acucgacugu gaacccagaa ccggccucga cuucuccgac uuguacuacc     1260

ucacaaugaa caacaaacac uggcuagucc acaaagaaug guuccacgac aucccccucc     1320

ccuggcacgc aggagcggac accggaaccc cacacuggaa caacaaagaa gcccucgucg     1380

aguucaaaga ugcccacgca aaacgccaga cagucgucgu ccuaggaagc caagaaggag     1440

caguacacac agcacucgca ggagcccuag aagccgaaau ggacggugca aaaggcagac     1500

ucagcagcgg acaucucaaa ugcagacuua aaauggacaa acuccggcuc aaaggcguca     1560

gcuacagccu cugcacagca gccuucacau ucacaaaaau ccccgcggaa acccuccacg     1620

gcacuguaac ggucgaaguc caauacgcag gaacggaugg cccaugcaaa gucccagccc     1680

aaauggcagu cgacaugcaa acccucaccc ccgucggccg ucucauaacc gcaaacccgg     1740

ucaucacaga aagcaccgaa aacagcaaaa ugaugcugga acuagaccca cccuucggag     1800

auuccuacau cgucaucggc guaggcgaaa aaaaaauaac ccaccacugg cacagaucag     1860

guucgacaau cggaaaagcg uucgaagcca ccguaagagg cgccaaacgc auggccgucc     1920

ucggagacac cgcaugggac uucggaucug uagguggagc ccuuaacucc cuaggcaaag     1980

guauccacca aaucuuugga gcugcauuca aaucacuauu cggcggaaug ucaugguuca     2040

gucaaauacu caucgggacc cuacucaugu ggcuaggccu aaauaccaaa aacgggucga     2100

uaucacuaau gugccucgca cucggcggcg uacucauauu ucucucaacc gcgguaagcg     2160

cagacguggg augcucaguc gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7276
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 2 opt

<400>  7276
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaaugc acgaaaggaa aagaaacgaa gaggagcaga cacauccgua ggaauagugg      120

gacuacuacu aaccacggcc auggcagcag aaguaaccag gaggggaucg gccuacuaca      180

uguaccucga cagaaacgac gcaggagaag caaucuccuu ccccacaaca cucggaauga      240

acaaaugcua cauccagaua auggaucuug gacacaugug cgacgccaca augagcuacg      300

agugccccau guuagacgaa ggcguugaac cagacgacgu cgauugcugg ugcaacacaa      360

ccucaacuug gguagucuac gggacaugcc aucacaagaa aggggaggcc cgcaggucca      420

gacgagccgu aacacucccc agccauucaa cacgcaaacu ccagacgcgc agccagaccu      480

ggcucgaaag ccgagaauac acaaaacacc uaauccgcgu agaaaacugg auuuuuagaa      540

accccggauu cgcacucgca gcagcggcua uagccuggcu ccucggaucc agcaccagcc      600

aaaaaguaau cuaccucgua augauccugc ucaucgcccc agcauacagc auccgaugca      660

ucggagugag caacagagac uucguagaag gcaugucagg cggcaccugg guagauguag      720

uacucgaaca cggaggaugc guaacgguaa uggcccaaga caaaccaaca guggacaucg      780

aacucgugac caccaccguc uccaacaugg cagaaguacg gucauacugc uacgaagccu      840

caaucagcga cauggcaagc gacuccaggu guccgaccca aggcgaagca uaccucgaca      900

aacaaagcga cacccaauac gucugcaaac ggacccuagu agaccgaggc ugggguaacg      960

gcugcggccu cuuuggaaaa ggaagccucg uaacaugcgc caaauuugcc ugcagcaaaa     1020

agaugacagg gaaaagcaua caaccagaaa accuagaaua cagaauaaug cucuccgucc     1080

acggcagcca acacucaggc augauaguaa acgacaccgg acacgaaacg gacgagaaca     1140

gagccaaagu cgaaauaacc ccaaacagcc cacgcgccga agccacgcuc gggggcuucg     1200

gaucauuggg auuagacugu gaaccccgca caggucucga uuucagcgac cucuauuacc     1260

ucaccaugaa caacaagcac uggcugguac acaaagaaug guuccacgac auaccacucc     1320

ccuggcacgc cggugcagac accggcacac cccacuggaa uaauaaagaa gcguuggucg     1380

aguucaaaga ugcccacgcg aagagacaaa ccguaguagu gcucggcagc caagaaggag     1440

ccguacacac cgcccucgcc ggcgcucucg aagccgaaau ggacggagcc aaaggucgcc     1500

ugagcuccgg ccaccuaaaa ugccgacuaa aaauggacaa acuuagacua aaaggaguau     1560

cauacucacu cugcacggca gccuuuaccu ucaccaaaau cccggcagaa accuuacacg     1620

gaacaguaac agucgaagug caauacgcag gcacagacgg ccccugcaaa guccccgcac     1680

agauggccgu agacaugcag acccucaccc cggucggacg ccucaucaca gcuaacccgg     1740

ugaucaccga auccaccgaa aacagcaaaa ugaugcucga acuugaccca cccuucggcg     1800

acagcuacau cgucaucgga gugggagaaa agaaaaucac gcaucacugg caccguagcg     1860

gaucaacgau aggaaaggcc uucgaggcca cgguaagagg cgcgaaaaga auggcagucc     1920

uaggagacac agccugggau uucgggucgg ucgguggcgc acucaacuca cuaggcaaag     1980

gcauccauca aaucuucggc gccgccuuca aaagccuauu cggaggaaug agcugguuua     2040

gccaaauacu uauaggcaca cuccuaaugu ggcucggacu caauacaaag aacggaagca     2100

ucucacuaau gugucuagcc cuaggcgggg uccucauauu ccuauccaca gucgucagcg     2160

ccgacgucgg cugcagcguu gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7277
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 2 opt

<400>  7277
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaaugc cagaaaagaa aagaaacggc gcggagccga cacauccguc gggauaguag      120

gacuccugcu cacaaccgca auggcagcgg aggucacucg acggggcuca gcguacuaca      180

uguaccucga cagaaacgac gcaggagaag caauaucauu ccccacaaca cuaggaauga      240

acaaaugcua uauccagaua auggaccuag gccacaugug cgacgcgaca augagcuacg      300

aguguccaau gcucgacgaa ggaguagaac ccgacgacgu agacugcugg ugcaacacca      360

ccuccgcaug gguaguguac ggaacaugcc accacaagaa aggcgaagcc agacgcucgc      420

gcagagccgu cacccuaccc agccacucca cccgaaaacu ucaaacccgc ccacaaacau      480

ggcuagaauc acgggaauac accaaacacc uaauacgcgu ugaaaauugg auauucagaa      540

accccggauu cgcacuagcu gccgcugcca uagccuggcu acuagggagu ucaacaucac      600

aaaaagucau cuaccuaguc augaucuuac ucaucgcacc cgccuacagc auccgcugua      660

uaggagucag caaucgagac uuuguagagg gaaugagcgg gggcaccugg guagacgucg      720

uccuagaaca cggcggaugc guaaccguca uggcgcaaga caaaccaacc gucgacaucg      780

aacucgucac aacaaccguc ucaaacaugg ccgaaguucg aucauacugc uacgaagcga      840

guauaucaga cauggccagc gacucccggu gccccaccca aggugaagca uaccuggaca      900

aacaaagcga cacccaguac gucugcaaaa ggacccuggu ggaccgcgga uggggcaacg      960

gcugcggacu cuucggcaaa ggcagccucg uuacgugcgc uaaauucgcc ugcagcaaga     1020

aaaugacagg caaauccauc caacccgaaa accuagaaua ccgaaucaug cuaagcguac     1080

acggcucgca acacucaggc augauaguca acgacaccgg gcacgaaacc gacgaaaacc     1140

gagccaaagu agaaaucacg ccaaacucac cccgagccga ggccacacuc ggcggcuucg     1200

gaucccucgg ccuagacugc gaacccagaa caggucucga cuucuccgac cuauacuacc     1260

ugacaaugaa uaauaaacac uggcucgucc acaaagagug guuccacgau aucccacugc     1320

ccuggcaugc cggcgcggac accggcacac cccacuggaa caacaaagaa gcccuaguag     1380

aguucaaaga cgcacacgcc aaaaggcaaa ccgucgucgu ccuaggaucc caagaaggcg     1440

ccguccacac cgcccucgcc ggcgcccuag aagcagaaau ggacggagcc aaaggccgcc     1500

ucucaucagg ccaccucaag ugccgccuaa aaauggacaa gcuacgacua aaaggcguga     1560

gcuacucccu cugcacagcg gccuucaccu ucacgaagau accagccgaa acacuccacg     1620

ggaccgucac cguagaggua caauacgcag gaacagacgg gcccugcaaa guacccgccc     1680

aaauggccgu agacaugcaa acccucaccc caguaggacg acucauaacc gccaacccag     1740

ucauaaccga gagcaccgaa aacagcaaaa ugaugcuaga acuagaccca cccuucggcg     1800

auaguuacau uguaauaggc guaggcgaaa agaaaaucac acaccauugg cacagauccg     1860

gaucaaccau uggcaaagca uucgaagcaa ccguacgcgg cgcuaaacgc auggccguac     1920

ucggcgauac cgcaugggac uuuggauccg ucgggggagc auuaaacuca cuaggaaaag     1980

gcauccacca gauauucggc gccgcauuca aaucacucuu cggaggaaug ucaugguuua     2040

gccaaauacu cauaggcaca cuccucaugu ggcuaggccu caacaccaaa aacggaagca     2100

uaucccuuau gugccucgcc cucggcggug uucucaucuu ucuauccaca gccgucagcg     2160

ccgacgucgg cugcucaguc gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7278
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 2 opt

<400>  7278
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc ccgcaaagaa aagaaacgac gcggcgcuga caccuccgua ggcauaguag      120

gacuccuacu caccaccgca auggcagcag aagucacccg ccgaggcagc gcauacuaca      180

uguaccucga cagaaacgac gccggugaag ccauaucauu ccccaccacc cucggaauga      240

acaagugcua cauccaaaua auggaccuug gccacaugug cgacgccacc augagcuacg      300

aaugcccaau gcucgacgaa ggaguagagc cagacgacgu cgacugcugg ugcaacacga      360

ccuccacuug ggucgucuau ggcacgugcc accacaagaa aggagaagcc agaagaucac      420

gccgugcagu aacgcuaccg agccacucaa cccgaaaacu ucaaacccgu agccagaccu      480

ggcuagaguc acgcgaauac acuaaacacc ugauccgcgu agaaaacugg auauuucgca      540

accccggcuu cgcgcucgca gccgcggcaa uagccuggcu gcuaggaucg uccaccucca      600

aaaagguuau auaccucgua augauccucc ucauagcucc cgcauacuca auaaggugca      660

uaggcgucag caaccgcgac uucgucgaag gcauguccgg aggcaccugg guagacgugg      720

uccucgaaca cggaggaugc gucacaguga uggcacaaga caaacccacc gucgacauag      780

aacucgucac cacuacaguc agcaacaugg ccgaagucag aucauacugc uacgaagcaa      840

gcaucagcga cauggcaucc gacucgcgcu gccccaccca aggcgaagca uaccuagaca      900

aacaaagcga cacccaguac guaugcaaac gcacacucgu cgaccggggc ugggggaacg      960

gcugcggucu cuucggaaaa ggcucccucg uaaccugcgc caaauucgca ugcuccaaga     1020

aaaugaccgg caaaagcauc caacccgaaa accucgaaua ccgaaucaug cuaucggucc     1080

acgguuccca acacagcgga augauaguca acgacaccgg acacgaaacc gacgaaaacc     1140

gagccaaagu cgaaauaaca cccaacagcc cgcgagcaga agccacacua ggcggauucg     1200

gaagccucgg ccuagacugc gagccccgca caggauuaga cuuuuccgac uuauacuacc     1260

uaacgaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auaccacuac     1320

cauggcacgc aggggccgac accggaacac cacacuggaa caauaaagag gcacucguag     1380

aauuuaaaga cgcacacgca aaacgacaga ccguugucgu acucggaucc caggagggag     1440

ccguccacac cgcacuagcc ggugcccuag aagcagaaau ggacggagca aaaggaagac     1500

uaaguagugg gcaccucaaa ugccgucuaa aaauggacaa gcucaggcua aaaggcguga     1560

gcuacucccu cuguacagcc gccuucaccu ucaccaaaau ccccgcugaa acacuacacg     1620

gaaccgucac cgucgaaguc caauacgccg gcaccgacgg ccccugcaaa guucccgccc     1680

agauggccgu cgacaugcaa acccucacac ccgucggucg acucaucaca gcgaaccccg     1740

ugauaaccga aagcacugaa aauagcaaaa ugaugcucga gcuggaccca cccuuugggg     1800

acagcuacau aguaaucggc gucggcgaaa agaaaaucac ccaccacugg cacagaagcg     1860

ggagcaccau aggaaaagca uucgaagcaa caguccgcgg ugcaaaacgu auggccguac     1920

ucggagacac agccugggac uucggcagcg uaggcggugc acuaaacagc cuaggaaaag     1980

gaauacacca aauauucgga gccgccuuca aaagccucuu cggaggcaug ucgugguucu     2040

cgcagauucu cauaggcaca cuucuaaugu ggcuaggccu aaacaccaag aacggcucaa     2100

ucagccuaau gugccuggca cucggaggcg uccuaaucuu ccuaucaaca gcagucagcg     2160

cagacgucgg augcagcgua gacuuuucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7279
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 2 opt

<400>  7279
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc acgaaaagaa aagaaacgac gcggagccga cacauccgua ggcaucguug      120

gacuacuauu aaccacagca auggccgccg aaguaacaag aagagguagc gcauacuaca      180

uguaucuuga ccggaacgac gccggcgaag ccaucagcuu cccgaccaca cuagggauga      240

acaaaugcua cauucaaaua auggaccugg gccauaugug cgacgcaaca augucauacg      300

aaugcccgau gcucgacgag ggcguagaac cggacgacgu cgauugcugg ugcaacacaa      360

cuucaacuug ggucgucuac gguaccugcc accacaagaa aggcgaagcc cgccgcucgc      420

ggcgcgcagu aacgcuaccc agccacucca cgcgcaaacu acaaacccgc ucccagaccu      480

ggcuagaauc gcgagaguac acaaaacacc uaauccgcgu ggaaaacugg auuuuccgaa      540

acccaggcuu cgcccucgca gccgcagcga ucgcauggcu ccuaggcagu agcacacgac      600

aaaaaguaau auaucuugua augauacuuc ucaucgcccc cgccuacagc auccgaugca      660

uuggcgucag caacagggac uucguagaag gcauguccgg aggaaccugg guagacgucg      720

uucucgaaca cggcggcugc gucaccguaa uggcacaaga uaaaccgacc guagacaucg      780

aacuugugac caccacggug uccaacaugg ccgaaguaag aagcuacugc uaugaagcaa      840

gcaucagcga cauggccagc gacagcagau gccccacaca aggcgaggcu uaccucgaua      900

aacaaucaga cacccaguac guaugcaagc gaacccuagu cgacagaggc uggggaaacg      960

gcugcggccu cuucgguaaa gggucacucg uuaccugcgc caaauucgcc ugcagcaaga     1020

aaaugaccgg caagagcauc caacccgaaa accucgaaua ccgaaucaug cugucagucc     1080

acgguagcca acacucaggu augauaguca acgacaccgg acacgaaaca gacgaaaaca     1140

gagcaaaagu cgaaaucaca cccaacagcc cuagagcgga agccacccuc ggaggcuucg     1200

gcagccuagg ccuagacugu gaaccacgca caggccucga cuucagcgac uuauauuauc     1260

ucacaaugaa caacaagcac uggcuagucc acaaagaaug guuccacgac auaccacuac     1320

ccuggcacgc aggagcagac accggcacac cccacuggaa caacaaagag gcacucguug     1380

aauuuaaaga cgcacacgcc aaacgacaaa ccgucguagu acuaggaagc caagaaggcg     1440

caguacacac agcccuagcc ggggcgcuag aagccgaaau ggacggcgcc aagggacgcc     1500

ucuccuccgg acaccucaaa ugucggcuaa aaauggacaa acuaagacua aaagggguau     1560

ccuacagccu cugcacggca gccuucaccu uuaccaaaau cccagccgaa acccuacacg     1620

ggacagucac aguagaaguu caauacgccg ggaccgacgg accaugcaag guccccgcgc     1680

aaauggccgu ggacaugcaa acccuaacac cuguaggccg acucauaacc gccaaccccg     1740

uaauaaccga aagcaccgaa aacuccaaaa ugaugcucga auuagauccc ccauucggcg     1800

acagcuacau aguaauaggc guaggcgaga aaaagaucac gcaccacugg cacagauccg     1860

gcagcaccau cggaaaagcc uucgaggcaa ccgugcgagg cgccaaacgc auggccgucc     1920

ucggagacac ggcaugggac uucggaucag uaggcggagc ccuaaacagc cuaggaaaag     1980

gaauacacca aauauucggc gcagccuuca aauccuuauu cggcggcaug agcugguucu     2040

cacaaauacu aaucgggacc cugcucaugu ggcucggacu caacaccaaa aacggaagca     2100

uaucccucau gugccucgca cucgggggcg uacuaaucuu ccugagcaca gcaguauccg     2160

ccgacguagg uugcucgguc gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7280
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 2 opt

<400>  7280
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaacgc gcgaaaagaa aagaagcgaa gaucggcaga caccagcguc ggcauagucg      120

gccuacuccu caccaccgcg auggccgcag aagucacacg caggggcucc gcauacuaca      180

uguaccucga ccgaaacgac gccggcgaag ccaucaguuu ccccacaaca cuaggaauga      240

acaaaugcua cauacaaauc auggaccugg ggcacaugug cgacgccaca auguccuacg      300

aaugccccau gcuggacgaa ggcguggaac cggacgacgu agacuguugg ugcaauacca      360

ccagcaccug gguaguguau ggcacaugcc accacaagaa aggagaagcc agacgcucac      420

ggcgcgcagu gacacuccca agccacagca cucguaaacu acaaacccga agccaaaccu      480

ggcuagaauc ccgagaauau acaaaacacc ucauaagagu agaaaacugg auauuccgaa      540

accccggauu cgcgcucgca gccgcagcaa uagcauggcu acuuggcuca agcaccagcc      600

aaaaagucau auaccuaguc augauacugc ucaucgcccc agcauacuca aucagaugca      660

uaggcguaag caaccgagac uucguugaag ggaugucugg agggacaugg gucgacgucg      720

uacuagaaca cggaggcugc gucacgguca uggcccagga caaaccaacg guagauauag      780

agcucguaac gacaaccgua agcaacaugg cagaaguacg caguuacugc uacgaagcca      840

gcauaucaga uauggccucc gacucccgcu gccccacaca gggagaggcc uaccucgaca      900

aacaaucaga cacacaauac gucugcaaac ggacgcuagu agaccgcggc uggggaaaug      960

gaugcggucu cuucggcaaa ggaucgcuag ugacaugugc caaauucgca ugcuccaaga     1020

aaaugacagg caaauccauc caaccagaaa accuagagua cagaauaaug cucagcguac     1080

acggcagcca acacagcggc augauaguca augacacagg acacgaaacc gacgaaaacc     1140

gggccaaggu agagauaacc cccaacagcc caagagcaga ggcgacccug ggcgguuucg     1200

gcagccucgg ccuagacugc gagccaagaa caggacucga cuucagcgac cucuacuacc     1260

uaacaaugaa caacaaacac uggcuaguac acaaagaaug guuccacgac auaccacuac     1320

cauggcacgc cggcgcagac accgggacac cacacuggaa caacaaggaa gcccuagucg     1380

aguucaagga cgcacacgca aaacgacaga ccgucguagu acuaggauca caagaagggg     1440

ccguacacac agcccuagcc ggugcacuag aagcagaaau ggacggagca aaaggacgcc     1500

uaucaagcgg ccaccuaaaa ugccgccuca aaauggauaa acuucgucua aaaggaguca     1560

gcuacagcuu augcacugca gcauucacau ucacaaaaau cccagcagaa acacuacacg     1620

gaaccgucac aguggaaguc caauacgccg gaacagacgg cccaugcaaa guaccagccc     1680

aaauggcggu cgauacccag acacucaccc cagucgggcg acucauaaca gcaaacccag     1740

ucaucaccga aucgacagaa aacucaaaaa ugaugcuaga acucgaccca cccuucggcg     1800

acagcuauau cguaauaggg guaggagaga aaaagauaac acaccacugg caccggagcg     1860

gcagcaccau aggcaaagcg uucgaagcca caguccgugg agcaaaaaga auggcaguau     1920

ugggggauac ggccugggac uucggcagcg uaggaggagc ccucaacagc cuaggaaaag     1980

ggauccacca aauauucggc gccgcguuca aaagccuguu cggaggaaug agcugguucu     2040

cccaaauacu aaucgggacc cuccucaugu ggcucggacu uaacaccaaa aacggaagca     2100

ucucacuaau gugccuagca cuaggaggag ugcuaauuuu ccugaguaca gccguauccg     2160

cggacgucgg cugcuccguu gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7281
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 2 opt

<400>  7281
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc aagaaaagaa aagaaaagac gaggagccga cacaaacgua ggaaucgucg      120

gacuacuccu cacaacugca auggcagcag aagucacgcg acgaggaucc gccuacuaca      180

uguaccuaga ccgcaacgac gcgggcgaag caauaucauu cccaaccacc cuggggauga      240

acaaaugcua cauacagaua auggaccucg gacacaugug cgacgcgacc augagcuacg      300

agugcccaau gcucgacgaa ggcguggaac cagacgaugu cgacugcugg ugcaacacca      360

cgagcacaug ggucguauac gggacaugcc accacaagaa aggagaagcg aggcgauccc      420

ggagagccgu cacccucccg agccacucaa cacgaaaacu acaaacaaga ucgcaaaccu      480

ggcuagaauc gagagaauau accaaacacc uaauaagggu agaaaacugg aucuuccgca      540

acccgggcuu cgcgcuagcc gcagccgcaa uagccuggcu gcuagggagc ucaacaagcc      600

agaaaguaau cuaucuagua augauccucc uaauagcccc agcauacagc auccgcugca      660

uaggcguaag caacagggau uucguagaag gaaugagcgg aggcacaugg guagacguag      720

ugcucgagca uggaggaugc gucacaguca uggcacaaga caaaccaaca gucgacauag      780

aacugguaac aaccacagug uccaacaugg cagaaguccg cagcuacugc uacgaagcca      840

gcauaagcga cauggcaagc gacagccggu gcccaacaca aggagaagcc uaccuagaca      900

aacaauccga cacacaauac guuugcaaac gcacguuagu agaccgcgga uggggaaacg      960

guugcggacu cuucggcaaa gguagcuugg ugacaugcgc caaauucgcc ugcaguaaga     1020

aaaugacagg caaaagcauc caaccagaga accuagagua cagaaucaug cucagcgucc     1080

acggcuccca acacagugga augaucguaa acgacacagg acacgagacg gacgaaaaca     1140

gagcaaaagu agaaaucaca cccaacagcc cacgggcaga agccaccuug ggaggauucg     1200

gaucccuagg ucucgacugc gaacccagaa ccggauuaga uuucuccgac cucuacuacc     1260

uaaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac aucccauuac     1320

cauggcacgc cggugccgac acaggaaccc cgcacuggaa caacaaagaa gcccuagugg     1380

aguucaaaga cgcccacgca aaaagacaaa caguaguggu ucuaggaucc caagaaggug     1440

caguccacac ugcacuggcg ggugcacucg aagccgagau ggacggcgca aaaggccggc     1500

uaucgagcgg ccaccuaaaa ugcaggcuca agauggacaa gcuaagacuc aaaggcguga     1560

gcuacucacu cugcaccgca gcguucacau ucaccaaaau cccagccgaa acccuacacg     1620

ggacagucac aguggagguc caguaugcag gcaccgaugg cccaugcaaa guaccggccc     1680

aaauggcagu agacaugcag acacuaacac cggucggccg acuaauaaca gcaaacccag     1740

uaaucacaga aagcaccgag aacucgaaaa ugaugcuaga acucgauccc ccauucggcg     1800

acagcuacau cguaaucggg guaggggaga agaaaaucac ccaccauugg caccggagcg     1860

gcagcacgau cgggaaagca uucgaagcca cgguacgagg agcgagacga auggcagucc     1920

ucggggacac cgcaugggac uucggcagug uuggcggagc auugaacucg cucgggaaag     1980

ggauacacca gauuuucggg gcagcauuca aaucacuauu cggagggaug agcugguuca     2040

gccagauccu caucggaacc cuacugaugu ggcuuggacu aaacaccaaa aacggaucca     2100

uaagccuaau gugccuagcc cugggaggag uccuaauauu ccuaaguaca gccguaagug     2160

ccgacguagg augcagcguc gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7282
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 2 opt

<400>  7282
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucauaaacgc ccgaaaagaa aagaaacgac gcggagcaga aaccucggua ggcauaguag      120

gacugcuacu aaccacagcg auggcagccg aaguaacaag aagagguuca gccuacuaca      180

uguaccucga ccgcaacgac gcaggcgaag cgaucagcuu cccaacgacg cuaggaauga      240

acaaaugcua cauacaaaua auggaccugg gccauaugug cgacgccacg augaguuacg      300

aaugccccau guuggacgag ggagucgagc cagacgacgu cgacugcugg uguaacacaa      360

cgagcacgug ggucgucuac ggaaccugcc accacaagaa aggcgaagcc cgacgaucaa      420

gacgcgcagu aacacuaccg agccacucca cacgcaaacu ccaaacaaga agccaaaccu      480

ggcucgaaag ccgagaauac acaaaacacc uaauccgcgu agaaaacugg aucuuccgca      540

acccaggcuu cgcacuagca gccgccgcaa uagcauggcu ccucgguagc agcaccagcc      600

aaaaagucau cuaucuagua augauacucc uuauagcgcc agcauacucc auucgaugca      660

ucggcguuag uaaccgcgac uucguagaag gaaugucggg uggaaccugg guagacgugg      720

uacucgagca cgguggcugc guaacgguga uggcgcaaga caaacccaca gucgacaucg      780

aacucgugac aacaacagua agcaacaugg ccgaaguccg gucauacugc uacgaggcau      840

caaucagcga uauggccuca gacagccgcu gccccacgca gggcgaagcc uaccucgaca      900

aacaguccga cacacaauac gucugcaaac gaacgcuagu cgacagggga ugggggaacg      960

gcugcggacu cuucggcaaa ggaucccuag uaaccugcgc aaaauucgcc ugcucaaaga     1020

aaaugacagg aaaaagcauu caaccagaaa aucuagaaua cagaaucaug cugucaguuc     1080

acggcucaca acacagcggc augauaguaa acgacacagg ccacgaaaca gacgagaacc     1140

gagccaaagu agaaaucacu cccaauagcc caagagcaga agcaacccuu ggaggguucg     1200

ggucacuugg acuagacugc gaaccacgaa ccggccuaga cuucagcgac cuuuacuacc     1260

ucaccaugaa caauaaacac uggcucguac auaaagaaug guuccacgac aucccacugc     1320

cguggcacgc aggcgccgac acagguacac cacacuggaa caacaaagaa gcacugguag     1380

aguucaaaga cgcccaugcg aaacgccaga ccgucguugu gcugggcuca caagaaggag     1440

ccguacacac agcacuggcc ggagccuuag aagccgaaau ggacggcgcg aaaggacgcu     1500

uguccuccgg ccaccucaaa ugccggcuaa aaauggacaa acuacgccug aaaggaguuu     1560

cguauucacu gugcacagca gcguucaccu ucaccaaaau accagccgaa acccuacacg     1620

gcacaguaac cgucgaagua caauacgcgg gaaccgacgg gcccugcaaa guuccagcac     1680

aaauggcggu agacaugcaa accuuaacac cgguaggcag acuaaucaca gccaacccag     1740

ucaucaccga aaguacggaa aauucgaaaa ugaugcuaga acuagacccg ccauucggag     1800

acagcuacau aguaaucggg gucggcgaaa agaaaauaac acaccacugg cacagaagcg     1860

gcagcacgau aggcaaagcc uucgaagcca ccgucagagg cgcgaaacgg auggcaguac     1920

uaggcgacac cgccugggac uucggcagcg ucggcggagc ccucaacagc uuaggcaagg     1980

gaauccacca aaucuucggc gccgcauuca agagccucuu cggcggcaug ucgugguucu     2040

cccaaauccu cauaggcacu cuacuaaugu ggcuaggacu caacacgaaa aacggcagca     2100

uaagccugau gugccucgcg uuaggcggag uccucauauu ccugagcaca gccgucucag     2160

cggacgucgg gugcagcgug gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7283
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 2 opt

<400>  7283
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaacgc ccgcaaagag aaaaagcgca gaggcgcaga cacauccgua ggcauaguag      120

gccuacuccu gacaacagca auggccgccg aaguaacccg ucgaggauca gccuacuaca      180

uguaucuaga ccgaaacgac gccggagaag cuaucagcuu ccccaccaca cucggcauga      240

auaaguguua cauccaaauc auggaccucg gccacaugug cgacgcaacc augagcuacg      300

aaugcccaau gcuggacgaa ggcguagaac ccgacgacgu cgacugcugg ugcaacacca      360

ccuccaccug ggucgucuac ggaaccugcc accauaagaa aggggaagcc agacggagcc      420

gacgugcggu aacgcucccc agccacucaa cucgcaaacu ccaaacccgc agccaaacgu      480

ggcucgaaag ccgggaauac accaaacacc uaaucagagu ggaaaacugg auauuccgaa      540

accccggauu cgcgcucgca gcagccgcga uagcuuggcu gcucggaagc uccaccuccc      600

agaaaguaau auaucuaguc augauccucc ucauagcacc cgccuacagc cuccgcugca      660

uaggcguauc caaccgagac uucguggaag gcaugagcgg cggcaccugg guagaugucg      720

uacucgaaca cggaggcugc gucaccguaa uggcccaaga caaacccaca guagacauag      780

aacuugucac aaccaccgua uccaacaugg ccgaagugcg guccuacugc uacgaagcca      840

gcauaucaga cauggccucg gacucacgau gcccaaccca aggcgaagcc uaccucgaca      900

aacaaagcga cacccaauac guaugcaaac gcacacucgu agacagagga uggggcaacg      960

ggugcggucu uuucggaaaa ggaucacucg ucacaugcgc aaaauucgcc ugcuccaaaa     1020

agaugaccgg aaaaucaauc caaccagaaa accuagaaua ccgcaucaug cucagcguac     1080

auggcuccca acacuccggc augauaguca acgacaccgg acacgaaacc gacgaaaacc     1140

gcgcaaaagu agaaaucaca cccaacagcc cacgagccga agccacccug ggaggguucg     1200

ggagccuagg acucgacugc gaaccgcgaa cgggacuaga cuucuccgac cucuacuacc     1260

uuaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac aucccauuac     1320

cauggcacgc cggcgcugau accggcaccc cacacuggaa caacaaagaa gcccucgucg     1380

aguucaaaga cgcucacgcg aaacgccaaa ccguagucgu acucggcuca caagaaggcg     1440

ccgugcauac cgcacuagca ggggcacuag aagcagaaau ggacggcgca aaaggccgcc     1500

ucagcucugg ccaccuuaaa ugcagacuaa aaauggacaa gcuacgccua aagggcguga     1560

gcuacucacu gugcaccgca gccuuuaccu ucaccaaaau ccccgccgaa acucuccaug     1620

gaaccguaac agucgaaguc caguacgccg gcacggacgg accaugcaaa guccccgcac     1680

aaauggccgu ugauaugcaa acacugacac ccgugggaag acucaucacc gccaacccag     1740

ucaucaccga aagcacagaa aacucaaaaa ugaugcugga auuagaccca ccauucggcg     1800

acucauacau cgugaucggg gucggagaaa agaaaauaac acaccacugg caccgcagcg     1860

gaucaacaau aggcaaagca uucgaagcca cagucagagg ggcaaaaaga auggcgguac     1920

uaggagacac agcaugggac uucggcucag ugggcggagc ccucaacucc cuuggaaaag     1980

gcauucacca aaucuucggg gcagcauuca aaucccuauu cggaggaaug uccugguucu     2040

cgcaaauacu caucggcacg cuccuaaugu ggcuaggacu aaacaccaaa aacggaagua     2100

uaucccuaau gugccucgcg cucggaggcg uacuaauuuu ccugagcacc gcagucagcg     2160

cagauguagg cugcuccguc gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7284
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 2 opt

<400>  7284
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaaugc acgaaaggag aaaaagcgca ggggagccga caccaacgua ggcauagucg      120

gccuacuucu aacaacagca auggcggccg aaguaacaag acgcggauca gccuacuaca      180

uguaccucga ccgaaacgac gccggcgaag ccaucagcuu ccccaccacc cucggcauga      240

acaaaugcua cauacagaua auggaccucg gucacaugug cgacgccaca augucauacg      300

aaugccccau gcuagacgag ggaguggaac cggacgacgu cgacugcugg ugcaacacca      360

ccagcaccug gguagucuac ggaaccugcc aucacaagaa aggcgaagcc agaagauccc      420

gacgcgcugu aacccucccc agccacagca cucgcaaacu ccaaaccagg ucacaaaccu      480

ggcuagaauc ucgagaguac acaaaacacu uaauacgagu agaaaauugg auguuccgaa      540

accccggauu cgcccucgcg gcagcagcaa uagccuggcu ccucggaucc uccacaucgc      600

aaaaagucau auaccuuguu augauccuac uaaucgcacc ggcauacagc auccgcugca      660

ucggagucuc aaacagagac uucgucgaag gaaugucggg cggcacaugg gucgacgucg      720

uucuugagca cggaggaugc gucacgguca uggcucaaga caaaccuaca guagacauag      780

agcugguuac aacaaccgug uccaacaugg ccgagguccg guccuacugc uacgaggcau      840

ccaucagcga cauggccucc gacucacgau gcccaaccca aggggaggcu uaccucgaca      900

aacaaagcga cacacaauac gucugcaaac gaacacuggu ugauagggga uggggcaacg      960

gcugcggccu uuuuggcaaa ggaagccugg ucacaugcgc caaguucgcc ugcagcaaga     1020

aaaugacggg caaaaguaua caaccagaaa accuggaaua ccgaaucaug cucagcgucc     1080

acgguuccca acacuccggc augauaguaa acgacacagg ccacgaaaca gacgaaaaca     1140

gagcaaaagu cgaaaucacc cccaacagcc cacgggccga agccacacug ggcgguuucg     1200

gcagccucgg acucgacugc gaaccgcgca cgggacucga cuucucagac cucuacuacu     1260

uaacaaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auuccccuac     1320

cauggcaugc cggagcggac accggcacac cccacuggaa caacaaagaa gcacuggucg     1380

aguucaaaga cgcacacgcc aaacggcaaa cagucgucgu ccuaggauca caagaaggag     1440

caguccacac ggcacuagcg ggcgcccuag aagccgaaau ggauggcgca aaaggccggc     1500

uaucaagcgg acaccuaaaa ugcagacuaa aaauggacaa acuuagacuc aaaggcguaa     1560

gcuacagcuu augcacagcc gccuucaccu ucacgaaaau ccccgcagaa acacuccaug     1620

guacagucac gguagaagug caauacgccg gcacagacgg gccaugcaaa gucccugcac     1680

aaauggcagu cgacaugcag acccuaaccc cagucggccg acucauaacg gccaauccag     1740

ucaucacaga gucaaccgag aacagcaaaa ugaugcucga gcucgacccg ccauucggcg     1800

acagcuauau cgucaucggu gucggcgaaa agaaaaucac acaccauugg cacaggagcg     1860

guagcacaau aggcaaggca uucgaagcaa ccguccgcgg cgccaaacgc auggccgucc     1920

uaggagacac cgccugggac uucggaucag uagggggcgc ccucaauagc cugggcaaag     1980

gcauccauca aaucuucggg gcagccuuca aaagccuuuu cgguggaaug uccugguuca     2040

gccaaauccu cauaggcacc cuccuaaugu ggcucggccu caacacgaaa aacgggucca     2100

uaucccucau gugucuagca cuaggcggag uccucaucuu ccucuccacc gcggucucgg     2160

ccgacgucgg augcucggua gauuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7285
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 2 opt

<400>  7285
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaacgc ccgaaaagag aaaaagcgcc gaggcgccga cacaagcguc ggcaucgucg      120

gacuucuccu aacaacagcc auggccgcgg aaguaacaag aaggggcagu gcauacuaca      180

uguaccucga ccgcaacgac gcaggagaag ccaucagcuu ccccacaaca uuaggcauga      240

auaaaugcua cauacagauc auggacuugg gccacaugug cgacgccaca augagcuacg      300

aaugccccau gcucgacgaa ggcguagaac cagacgacgu cgacugcugg ugcaacacaa      360

caagcacgug ggugguauac ggaaccugcc accacaagaa aggagaagca agacgaagcc      420

guagagcagu gacacucccc agccacucga ccagaaaacu gcaaacacgc agccaaaccu      480

ggcucgaauc acgagaauac accaaacacc ucauccgagu cgaaaacugg auacuacgga      540

accccggauu ugcacucgca gcagccgcca ucgcauggcu acucggcuca ucgaccuccc      600

agaaagucau auaccuugua augauacucc uaaucgcgcc cgccuacagc auccgaugca      660

ucggcguguc uaaucgagac uucguagaag gcaugagcgg aggcacaugg gucgacgucg      720

uacuggagca cggaggcugc gugacgguaa uggcacagga caaacccaca gucgacauag      780

agcuagucac aacgaccguc uccaacaugg cagaaguacg cagcuacugu uacgaagcca      840

gcauaagcga cauggccagu gacagccggu gcccaaccca aggggaagcc uaccuagaca      900

aacaaagcga cacacaauac guaugcaaaa gaacccucgu cgaucgaggc uggggaaacg      960

gaugcggccu cuucgggaaa ggaucccucg ugaccugcgc caaauucgca ugcagcaaga     1020

aaaugaccgg aaaaaguaua caaccagaaa accuagagua ucgcauaaug cuaucagucc     1080

acggcuccca acacuccggg augauaguca acgacaccgg ccacgaaaca gacgaaaauc     1140

gcgcaaaagu cgaaaucacc ccaaacagcc caagagcuga agccacccua ggaggauucg     1200

gaucccucgg ccucgacugc gaaccacgaa caggccucga cuuuuccgac uuauauuacc     1260

ucacaaugaa caacaaacac uggcuagucc acaaggaaug guuccacgac aucccacuac     1320

ccuggcacgc aggcgcagac acaggcaccc cacauuggaa caacaaagaa gcacucgugg     1380

aguucaaaga cgcucacgca aaacgccaaa caguaguagu ccuaggcuca caagaaggug     1440

ccguccacac agcucucgcc ggagcccuag aagcagaaau ggauggagca aaaggccgcc     1500

ugucgagcgg ccaccucaaa ugucgacuaa aaauggacaa acuacgccua aaaggcguca     1560

gcuacagcuu augcacagca gccuucaccu ucacaaaaau accggcggaa acacuccacg     1620

gcacggucac gguugaaguc caauacgccg gcacagacgg ucccugcaaa gucccagcac     1680

aaauggcagu cgacaugcaa acccuaaccc caguagggcg ucuuaucaca gccaacccag     1740

ucauuaccga gucaacagaa aacaguaaaa ugaugcuaga acucgaccca cccuuugggg     1800

acucguacau cgucaucgga auaggagaaa agaaaauaac ccaccacugg caccgaagcg     1860

gcuccaccau aggcaaagcc uucgaagcaa caguaagagg agcaaaacgc auggcaguac     1920

uuggagacac cgcgugggac uucggaagcg ucggaggcgc gcucaacagc cucggcaaag     1980

gcauccacca gaucuucggc gccgcguuca aaagccuguu cggaggaaug ucaugguucu     2040

cccagauacu aaucggaacc cuccuaaugu ggcuagggcu gaacacgaaa aacggaucga     2100

uaagccuaau gugucucgcc cuaggaggcg uacuaauauu ccucuccaca gcaguaagcg     2160

cagacgucgg cugcuccgua gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7286
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 2 opt

<400>  7286
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uaauaaacgc acgaaaagag aaaaagcgaa gaggagccga cacauccguc ggaaucgucg      120

gacugcuauu aacaacagcc auggccgcag aagucacccg cagaggcucc gccuacuaca      180

uguaccuaga ccgaaacgac gcgggugaag caaucuccuu ccccaccaca cucgguauga      240

acaaaugcua cauacagauc auggaccucg gccacaugug cgacgcaacg auguccuacg      300

aaugcccaau gcuagacgaa ggcguagaac ccgacgacgu cgacugcugg ugcaacacaa      360

ccaguacaug ggucguauac gggaccugcc accacaagaa aggagaagcc cgaagauccc      420

ggcgggcagu aacccuaccg ucacacucca cacgcaaacu ucaaacgcga agccaaaccu      480

ggcuagagag uagggaauau acaaaacacc uaauaagagu agagaacugg auauuccgca      540

accccggauu cgcgcucgcg gccgccgcaa uagcauggcu acucggcagc agcaccagcc      600

agaaaguaau auaccuaguc augauccuac ucauagcccc ggcuuacuca auccgcugca      660

uaggggucag caaccgcgac uucguagaag gcaugagcgg aggaacaugg guggauguag      720

uacuagagca cggcgggugc guaaccguaa uggcacaaga caaaccuacc gucgacaucg      780

aacucguaag cacaacugua uccaacaugg ccgaaguaag gucuuacugc uacgaagcca      840

ccauauccga caucgccucc gacuccagau gcccaacgca aggggaagcc uaccuagaca      900

aacaauccga cacacaauau guaugcaaac gaacccuagu cgacagagga uggggaaacg      960

gcugcggacu auucggcaaa ggaagucugg uaaccugcgc aaaauucgca ugcuccaaga     1020

aaaugacagg aaaauccaua caacccgaaa accucgaaua ccgcaucaug cuaagcgucc     1080

acggaagcca acacagcgga augaucguca acgacaccgg acacgaaacc gacgaaaacc     1140

gagccaaagu agaaaucaca ccaaacagcc cccgcgccga agccacccuc ggcggcuucg     1200

gcucccuagg acuggacugc gaaccacgaa ccggccuaga cuucagcgac cuauacuacc     1260

uaaccaugaa caacaaacac uggcuagucc acaaagagug guuccacgac aucccauugc     1320

ccuggcacgc cggagccgac accgguacac cccacuggaa caacaaagaa gcccuaguag     1380

aauuuaaaga cgcccacgcu aaaagacaaa ccgcagucgu ccucggaucc caagaaggcg     1440

ccguacacac agcacuagca ggcgcgcucg aagccgaaau ggauggcgcg aaaggaagac     1500

ucagcagcgg gcaccucaaa ugccgguuaa aaauggacaa acuccgccuc aaaggcgucu     1560

cauacagccu cugcaccgca gcuuucaccu ucacuaaaau ccccgcggaa acucuacacg     1620

ggacagugac cgucgaaguc caauacgccg gaacagacgg cccaugcaag guaccagcac     1680

aaauggcagu cgacaugcaa acccuaaccc cgguaggaag auuaauaacg gccaacccug     1740

uaauaaccga aagcaccgaa aacagcaaga ugaugcucga acucgauccc ccuuuuggcg     1800

acagcuacau cguaaucggc gucggcgaaa agaaaaucac ccaccacugg caccgauccg     1860

gauccacaau cggcaaagcc uucgaagcaa cgguccgggg agccaaacga auggcggucc     1920

ugggagacac ggcaugggac uucggauccg uagguggggc ccuaaacucg uuaggcaaag     1980

gcauccacca gaucuucggc gcagccuuca aaucacucuu cgggggaaug agcugguucu     2040

cgcaaaucuu aaucggaaca cuucuaaugu ggcucggccu uaacaccaaa aacggaagca     2100

ucucgcugau gugccuagca cuaggcggag uccucauauu ccuaagcaca gccguauccg     2160

cagacguggg cugcagcguu gacuuuagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7287
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 2 opt

<400>  7287
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauaaacgc acgcaaggaa aagaaacggc gcggcgccga cacauccgua ggcaucgucg      120

gcuuacuccu aacaacagca auggcagccg aaguaacaag gcgaggaagu gccuacuaca      180

uguaccugga caggaacgac gcaggagaag ccaucagcuu cccaaccacu cucggcauga      240

acaaaugcua uauacaaauc auggaccuag gacauaugug cgacgcaacc augagcuacg      300

aaugccccau gcuagacgaa ggaguggaac cagacgaugu agacugcugg ugcaacacaa      360

ccucaacaug ggucguauac ggaaccugcc accacaagaa aggugaagcc cgacgcucaa      420

gacgagcggu aacacucccu agccacucua cccgaaaacu ccaaacccgc agccaaacau      480

ggcucgaauc ccgagaguac acaaaacacc uaauacgagu agaaaacugg auauuccgaa      540

acccgggauu cgcccuagcc gcagccgcaa ucgccuggcu ccuaggcagc agcaccucgc      600

aaaaagucau cuaccuaguc augauacuac ucauagcccc cgccuacagc auccgcugca      660

uaggaguauc gaaccgggac uucguagagg gcaugagcgg cggcaccugg gucgacguag      720

uacuagaaca cggcgggugc guuacgguca uggcacaaga caaacccacg gucgacaucg      780

agcuagucac gaccaccguc ucaaacaugg cagaaguccg cuccuacugu uacgaagcau      840

caaucagcga cauggcgucc gacuccaggu gcccgaccca aggagaagcc uaucucgaca      900

agcaaagcga cacacaguau guuugcaagc gcacccuugu cgacaggggc ugggguaacg      960

gcugcggccu cuucgggaag ggcucccuag ucacaugugc aaaauucgca ugcagcaaga     1020

aaaugaccgg caaauccauc caaccagaaa accucgaaua ccgcauaaug uuaagcguac     1080

acggcuccca acauuccggc augaucguua acgacaccgg acacgaaaca gacgaaaacc     1140

gagcaaaagu cgaaauaaca ccaaacagcc cccgagcaga agccacccua ggaggauucg     1200

gaucacucgg acuagacugc gaaccgcgaa ccggacucga cuucucagac cuuuacuacc     1260

uaacaaugaa caacaaacac uggcucguac acaaagaaug guuccacgac auaccacugc     1320

ccuggcacgc cggcgcagac acaggaacgc cacacuggaa caacaaagaa gcccuagucg     1380

aguucaaaga cgcacacgcc aagcgacaaa cagugguagu acucggaacc caagaagggg     1440

ccguccacac cgcccuggca ggagcacucg aagccgaaau ggacggggcg aagggacgcc     1500

ucuccucagg acacuuaaaa ugcagacuua agauggacaa acuccgacua aaaggaguca     1560

gcuacagccu augcaccgca gcauucacgu ucaccaaaau accagcugaa acacuccacg     1620

gcacggucac agucgaaguc caauacgccg gcacagacgg accaugcaaa guacccgccc     1680

agauggccgu ggacaugcaa acgcucaccc ccgucggacg ccucauaacc gccaaccccg     1740

ucauaaccga auccaccgaa aacagcaaaa ugaugcuaga acucgacccu cccuucggag     1800

acucauacau cgucauaggc gucggcgaaa agaaaaucac gcaccauugg cacagauccg     1860

gaucaacaau aggcaaagca uucgaagcaa cagugagagg ggccaagcga auggcagucc     1920

uuggagacac agcaugggac uucggaagcg uagguggugc gcuaaacucc cuaggcaaag     1980

gaauccacca aaucuucgga gcagcauuca aaagccuuuu uggcggaaug ucgugguuca     2040

gccaaauccu aaucgggacg cugcuaaugu ggcuaggacu aaacacgaaa aacggaucca     2100

ucucgcugau gugccuagca cuaggcggag uccugaucuu ccuuucaacc gccgucuccg     2160

cagacguagg cugcucaguc gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7288
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 2 opt

<400>  7288
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaaucaacgc ccgaaaagaa aagaaaagac gaggggcaga cacauccguc ggcauaguag      120

ggcuccuccu cacaacagcc auggccgcag aaguaacccg acgaggcagc gcauacuaua      180

uguaccucga caggaacgac gccggugagg caauaucguu ccccacgacc cucgggauga      240

acaagugcua uauacagauc auggaccucg gccacaugug cgacgcaaca augucauacg      300

aauguccaau gcucgacgag ggaguagaac ccgaugacgu ugacugcugg uguaacacca      360

ccagcacgug gguugucuac ggaaccugcc aucacaagaa aggcgaagcc cgacgcuccc      420

gcagagccgu cacacugccc ucgcauucaa caagaaaacu ccaaacacgc ucccaaacau      480

ggcucgaauc ccgcgaauau acaaaacacc ucauccgcgu agaaaacugg auauucagga      540

aucccggguu cgcccuagcc gccgcagcaa ucgccugguu gcucggcuca agcaccagcc      600

aaaaaguaau auacuuaguc augauccuac ucaucgcccc cgcguacagc auccgcugca      660

ucggagucag uaaccgggac uucgucgaag gcauguccgg aggaacaugg guggacgucg      720

uacuagagca cggaggcugc gugaccguaa uggcacaaga caaacccaca guagauauag      780

agcuaguaac gacaaccgua uccaacaugg cagaaguucg aagcuacugc uaugaagcaa      840

gcaucagcga cauggcaagc gacucccgau gccccaccca gggagaagcc uaccuagaca      900

agcaauccga cacacaauac guaugcaaac gaacccucgu cgaccgcgga uggggaaacg      960

gcugcggccu auucgggaaa ggaucccuag uaacaugcgc aaaauucgca ugcagcaaga     1020

aaaugaccgg uaagagcauc caacccgaaa accucgaaua ccgaauaaug cucaguguac     1080

acggcuccca acacagcggc augaucguaa acgacacagg acacgaaacc gacgaaaacc     1140

gugcgaaagu ggaaauaacc ccgaacuccc ccagagccga agcaacccua ggcggcuucg     1200

gaucacuggg ccucgacugc gaaccaagaa ccggacuaga cuucuccgac cuauacuacc     1260

ucacaaugaa caacaaacac uggcucguac acaaggaaug guuccacgac auaccccucc     1320

ccuggcacgc cggagcagac acagggacac cgcacuggaa caauaaagaa gcccuggucg     1380

aguucaaaga cgcucacgca aaacgccaaa cugucgucgu ccuuggcagc caagaaggcg     1440

ccguccacac agcacucgcc ggugcccucg aagccgaaau ggacggagcc aaagggcggc     1500

uaagcagcgg gcaccugaaa ugccggcuua aaauggacaa acuccgacug aaagguguau     1560

cauacagccu augcaccgca gccuucacau ucacaaaaau acccgcagaa acacuccacg     1620

gaaccgucac agucgaacuc caauacgccg gaaccgacgg acccugcaaa gugccagccc     1680

aaauggcagu agacaugcaa acgcucacuc ccguaggccg ccuuauaacu gcgaaccccg     1740

ucauaaccga aagcacagaa aacagcaaaa ugaugcucga acucgauccc ccguuugggg     1800

acucauacau cgucauuggc gugggcgaaa agaaaaucac gcaccacugg caccgcucag     1860

gcagcacuau cggaaaagca uucgaagcaa ccguccgcgg cgccaagaga auggcuguac     1920

ucggcgacac cgcaugggac uucggaucgg ucggaggggc ccucaacagc cucggcaaag     1980

gaauacacca aaucuucggc gccgccuuca aaucucuauu cggagggaug ucgugguuuu     2040

cccagauccu gauaggcacc cuccucaugu ggcuaggccu caacacgaaa aacggcucca     2100

uuagccuuau gugccucgcc cuaggcggcg uauuaaucuu ccucucaaca gcagucagcg     2160

ccgacguagg gugcagcguc gacuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7289
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 2 opt

<400>  7289
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc ccgcaaagaa aagaagcgca gaggagcaga aaccuccguc ggcauagucg      120

gccugcuacu aaccaccgcc auggccgccg aagucaccag acgaggcucc gccuacuaca      180

uguaccucga cagaaacgac gccggcgaag ccaucuccuu cccuacaacc cucggcauga      240

acaaaugcua cauacaaaua auggaccuag gccacaugug cgacgccacu augagcuacg      300

aaugccccau gcucgacgaa ggagucgaac ccgacgacgu ggauugcugg ugcaacacca      360

caucgacaug ggucguguac gggacgugcc accacaaaaa gggagaagcc agacgaucac      420

gcagagccgu cacacucccc agccacagca cgagaaaacu acaaacacgu agccaaacgu      480

ggcucgaaag ccgcgaauac accaagcacc ucauccgcau cgagaacugg auauuccgca      540

accccggcuu cgcacuagcc gcggccgcca ucgcauggcu acucggaucg agcaccucac      600

aaaaagucau auaccuaguc augauccuac uaauagcccc cgcauacucc auacgcugca      660

uuggcgucag caaccgggac uucguagaag gcaugagcgg uggcacaugg gucgacgucg      720

uccucgaaca cggaggcugc guaaccguca uggcucaaga caaaccuacg guagacauag      780

aacugguuac cacaacaguc agcaacaugg ccgaaguccg cagcuauugc uacgaagcaa      840

gcauaucaga cauggccagc gacucccguu gcccaacaca gggagaggca uaccuagaca      900

aacaauccga uacacaguac guaugcaaac guacccucgu cgaccgcggu uggggcaaug      960

gcugcggccu auucggaaaa ggaagccucg ucacaugugc aaaauucgcc ugcaguaaga     1020

agaugacagg caaaucaauc caacccgaaa accuagaaua cagaauaaug cucucggucc     1080

acggaagcca acacuccgga augaucguaa acgacaccgg acacgaaacc gacgagaacc     1140

gcgccaaggu cgaaaucacc cccaacucac cacgcgcgga agcaacacug ggaggcuucg     1200

gaucacucgg acucgacugc gaaccccgaa ccggauuaga cuuuuccgac cucuacuacc     1260

ucacaaugaa caauaaacac uggcucgucc acaaggaaug guuccaugac auaccacuac     1320

ccuggcaugc cggcgcggau acgggaacgc cccacuggaa caauaaggag gcccucgugg     1380

aguucaaaga cgcccacgca aaacgccaaa ccguaguagu gcucggcagc caagaaggag     1440

cuguccacac cgcccuggcc ggagcccucg aagccgaaau ggacggggcg aaaggacgcc     1500

ucuccucggg ccaccugaaa ugccgccuaa aaauggacaa acuccgauua aaagggguca     1560

guuacagccu augcaccgcg gcauucaccu ucacaaaaau accagcagaa acacuacaug     1620

gaaccgucac agucgaaguc caguacgccg gcacagacgg uccauguaaa guccccgcac     1680

aaauggccgu cgacaugcaa acacugacuc cagucggaag acucaucacc gcaaacccag     1740

uaauaaccga aucaaccgaa aacuccaaaa ugaugcucga acucgaccca ccauucggcg     1800

acuccuacau aguaauaggc gucggagaaa agaaaauaac acaccacugg caccgaagcg     1860

gauccaccau uggcaaggcu uuugaagcaa ccguacgagg agccaaacgc auggccgugc     1920

uaggagacac cgccugggac uucggcuccg ucggcggcgc ccuaaacucc cugggcaaag     1980

gcauacauca aauauucggc gccgcauuca aaucacucuu cggaggcaug agcugguuca     2040

gccagauccu cauaggcacc cuccuuaugu gguugggccu caacaccaag aacggaagca     2100

ucucccucau gugccuagca cucggcggcg uacucauauu ccucucaaca gccgucagcg     2160

ccgacguagg cugcagcguc gacuucucua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7290
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 2 opt

<400>  7290
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc acgaaaggaa aagaaacgcc gaggcgcgga cacaagcgug ggaauagucg      120

gacuccuacu cacaacggca auggcagccg aagucacacg aaggggcuca gcuuacuaca      180

uguaccugga cagaaacgac gccggggaag caaucagcuu uccgaccaca cuaggaauga      240

auaaaugcua cauacaaaua auggaccucg gacacaugug cgaugcaacc augucguacg      300

aaugcccaau gcuugacgag ggcguagagc ccgacgacgu cgauugcugg uguaacacca      360

cgagcacaug gguaguuuau ggaacaugcc accacaagaa aggagaagca cgcagaagca      420

gacgcgcugu cacgcuccca agucacagca cgcgcaagcu ccagacccgg agccagaccu      480

gguuagaaag ccgcgaauac accaaacacc ucaucagagu cgaaaacugg aucuucagaa      540

acccaggguu cgcacuagca gccgccgcca uagccuggcu acucggaucc uccacgagcc      600

agaaaguaau auaccucgua augauacucc ugaucgcacc agcauacucg auccgcugca      660

uaggcguaag caaccgggac uucguggaag ggaugucagg aggaaccugg gucgauguag      720

uucuugagca cggaggaugu gugacgguaa uggcucagga caaacccacg guagacauug      780

aacucgucac caccaccgug agcaacaugg cagaagugcg aucauacugc uacgaggccu      840

ccauauccga cauggccagu gacucaagau gcccaacgca aggagaagcg uaccuagaca      900

aacaaucaga cacgcaguac gucugcaaac gaacgcuagu cgaccgcgga ugggggaacg      960

ggugcggacu auuuggcaaa ggcucacugg ugacaugcgc caaauuugca ugcucaaaga     1020

aaaugacagg caaauccaua caacccgaga accucgaaua ucggaucaug cuauccguac     1080

auggcucaca acauagcggc augauaguca acgacacagg acacgaaaca gacgagaaca     1140

gagccaaagu cgaaauaacc ccaaauagcc cccgcgcgga agcaacccuc ggaggauucg     1200

gaagccuagg acuggacugu gaaccaagaa caggcuugga cuuuagcgac cucuacuacc     1260

ugacaaugaa caacaaacac uggcuagucc acaaagaaug guuccacgac auaccgcuac     1320

cguggcacgc aggggccgac accggcaccc cgcauuggaa caacaaagag gccuuagucg     1380

aguucaaaga cgcacacgca aaaagacaaa cgguaguagu acucggcucg caagaaggcg     1440

ccguacacac cgcgcucgca ggagcccucg aagcggaaau ggacggagca aaaggucgcc     1500

ugagcuccgg ccaucucaaa ugcagacuaa aaauggacaa acugagacuc aaagggguau     1560

cuuacagccu augcacggca gccuucacau ucacaaaaau cccugccgag acacuacacg     1620

ggacggucac agucgaggua caauacgccg gaaccgacgg accaugcaag guacccgccc     1680

aaauggccgu cgacaugcaa acacuaacac cagucgggcg ccuaaucaca gcuaaucccg     1740

ucaucacuga aucaaucgaa aacagcaaaa ugaugcuaga acuagauccg cccuucggcg     1800

acagcuacau cguaaucggc gucggagaaa agaaaaucac acaccacugg cauagaagcg     1860

ggagcacaau aggcaaagca uucgaagcca caguacgcgg agcgaaacga auggcagucc     1920

ucggcgacac cgcgugggau uucgggagcg ucggcggcgc acucaacucg cucggaaaag     1980

gaauccacca aaucuucgga gccgcauuca aaagccuauu uggcggcaug agcugguuua     2040

gccaaauacu caucggaaca cuccugaugu ggcucggacu caacacgaaa aacggcagca     2100

uaaguuugau gugccucgcc cucggagggg uacucauauu ucugagcaca gccgucagcg     2160

cagacguagg gugcagcgug gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7291
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 2 opt

<400>  7291
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucauaaacgc ccgcaaagaa aagaaacgca gaggcgcaga cacaucaguc ggcaucguag      120

gacuucuacu aacuaccgcg auggcagcag agguaacccg ccgcggaucc gcauauuaca      180

uguaccucga ccgcaacgac gcgggcgaag caaucucauu cccaacaacc cugggaauga      240

acaaauguua cauccaaauc auggaccucg gacacaugug cgacgcaacc auguccuacg      300

aaugccccau gcuagacgaa ggcgucgaac cagacgacgu cgacugcugg ugcaacacca      360

ccagcacaug gguuguauac ggcacguguc aucacaagaa aggagaagcg cgccgaagcc      420

gacgcgcagu gacgcugccc ucccacucca cccgcaaacu gcaaacgcgc ucccagacau      480

ggcuagagag ccgagaguac accaaacacc uaauccgagu agaaaacugg aucuuccgca      540

acccaggcuu cgcacucgcc gccgccgcaa ucgccuggcu acucggaucu agcaccagcc      600

aaaaaguaau cuaccucguc augauccucc ugaucgcucc cgccuacagc auccggugca      660

ucggggucag caacagagac uucguagaag gaauguccgg gggaacaugg guagacgucg      720

uccuagaaca cgggggcugc guaacuguca uggcucagga caaacccaca gucgacauag      780

aacuugucac caccaccguc agcaauaugg cggaaguacg cagcuacugu uacgaagcaa      840

gcauaucaga cauggccucu gacagccguu gcccgaccca aggagaagca uaucucgaca      900

aacaguccga cacccaauac gucugcaaac gaacccucgu agaccgagga ugggggaacg      960

gauguggacu cuucggcaaa ggaagccucg uaaccugcgc aaaauucgca uguuccaaga     1020

aaaugaccgg caaaucaauc caaccagaaa accucgaaua ccgaaucaug cucagcgucc     1080

acggauccca gcacuccggc augaucguaa acgauacugg ccacgaaacc gacgaaaacc     1140

gagccaaagu cgaaauaacc cccaauagcc cacgcgccga agccacccua ggcggauucg     1200

gcucccucgg ccucgacugc gaaccacgca ccggccuaga cuucagcgac cucuauuacc     1260

uaaccaugaa caacaaacac uggcuggucc acaaagaaug guuccacgac aucccccucc     1320

cuuggcacgc cggggcggac acaggaaccc cacacuggaa caauaaagaa gcacucgucg     1380

aguucaaaga cgcacacgcu aaaaggcaaa ccgucguagu acuaggcagc caagaaggag     1440

caguucacac cgcauuagcc ggcgcacuag aagccgaaau ggacggaguc aaaggacgcc     1500

ucuccuccgg acaccuaaaa ugccgccuca aaauggacaa acuacgccua aaaggaguaa     1560

guuauucacu cugcacagca gccuucacau ucaccaaaau cccagcagaa acccuacacg     1620

gcacagugac aguggaaguc caauacgcag gcaccgacgg cccaugcaaa gucccugcac     1680

agauggccgu cgacaugcaa acccucacac cagucggcag gcucaucacc gcuaacccag     1740

uaaucaccga aucaaccgaa aacucaaaga ugaugcucga acucgacccg cccuuugggg     1800

acuccuacau cgugaucggg guuggggaaa agaaaaucac ccaccacugg caccguucag     1860

gaucaacaau cggcaaagca uucgaagcaa ccguacgagg cgcaaaaaga auggccguac     1920

uaggcgacac agcaugggac uucggcucag ucggcggggc ccuaaacucc cucggcaaag     1980

gcauacacca aaucuucgga gcagcauuca agucccucuu cggcggcaug agcugguuca     2040

gccaaauccu caucggcaca cugcuaaugu ggcugggacu caacaccaaa aacggaucga     2100

ucucauucau gugccuggca cucgguggcg ugcucauauu ccucagcaca gccgucuccg     2160

cagacgucgg cugcagcgua gauuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7292
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 2 opt

<400>  7292
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagga       60

uaauaaacgc ccgaaaggaa aagaaacgac gaggagccga cacauccguc ggcaucguag      120

gacuccuccu uaccaccgcc auggcggcgg aggucacacg cagaggguca gccuacuaca      180

uguaccuaga ccgcaacgac gccggcgaag cgaucaguuu ucccaccacg cugggaauga      240

acaaaugcua cauccaaauc auggaccuag gacacaugug cgacgccaca augucauacg      300

aaugccccau gcucgacgaa ggcgucgaac cagacgacgu cgacugcugg ugcaacacga      360

ccuccacaug ggucguguac ggaaccuguc accacaagaa aggcgaagcc cgacgaucac      420

gcagagccgu cacccuaccc ucccacagca cccgcaaacu ucaaaccaga ucacaaaccu      480

ggcucgaauc ccgagaguau acaaaacacc ucauacgagu cgaaaacugg aucuuccgca      540

acccaggauu ugcccuggcg gccgcugcca uagccuggcu acucggcucc ucaaccucac      600

aaaaggucau auaccucguc augauccucc uaaucgcccc cgccuacagc auccgcugua      660

uaggagucuc aaacagagac uuuguagaag gaaugucagg cgggaccugg guugacgugg      720

uacuggaaca cggcggcugc guaacuguca uggcgcaaga caaacccaca guggacauag      780

agcuaguaac caccaccgua agcaacaugg cagaaguacg cuccuacugc uacgaagcau      840

cgaucuccga cauggccuca gacucccgcu gccccaccca aggcgaagca uaccucgaca      900

aacaaucaga cacccaauac gucugcaagc gcacccucgu agaccgcgga uggggcaacg      960

gcugcggccu guucgggaaa ggcucccuag uaaccugcgc caaauucgca ugcagcaaaa     1020

agaugacugg caaauccauc caacccgaga accucgaaua cagaaucaug cucucagucc     1080

acggauccca acacuccggc augauaguca augacacggg acacgaaaca gacgaaaacc     1140

gcgcgaaagu cgagaucacc ccaaacucac cccgagcaga agcaacccuu ggaggauucg     1200

gcagccuagg acucgacugc gaacccagaa ccggccuaga cuucuccgac cucuacuauc     1260

ucaccaugaa caacaaacac ugguuagucc auaaggaaug guuccacgac aucccccucc     1320

ccuggcacgc aggcgcugac accggcacac cccacuggaa caacaaagag gcacucgucg     1380

aguucaagga cgcccacgca aaacgacaaa caguagucgu acucggcucc caggaaggcg     1440

caguacacac agcccuagca ggcgcacucg aagcggaaau ggacggagca aaagguaaac     1500

uuucaagcgg ccaccucaaa ugccgacuga aaauggacaa gcugcgccua aaaggaguca     1560

gcuacagucu cugcacggcc gcauucacau uuacaaaaau accagccgaa acgcuacacg     1620

gcaccgucac cgucgaaguc caauacgccg gcaccgacgg ccccugcaaa guacccgcac     1680

aaauggccgu agacaugcaa acccucacac cgguaggacg ccucaucacc gcaaaccccg     1740

ucauuacaga auccaccgaa aacucaaaaa ugaugcucga acuagauccc ccguuuggug     1800

acuccuacau cgucaucgga guaggagaaa agaaaauaac acaccacugg caccgcagcg     1860

gaucgacaau cggaaaagcc uucgaggcaa ccgugcgcgg cgcaaaacga auggccgucc     1920

ucggagacac cgccugggac uucggcuccg ucggcggggc acuaaacagc cuaggaaaag     1980

gaauccauca gauauucgga gcagccuuca aaucacuguu cggaggcaug uccugguucu     2040

cccaaauacu gauaggcaca cuacucaugu gguuaggccu caacaccaaa aacggaagca     2100

uaagccucau gugccucgcc cucggcggcg uccucauauu ccucuccaca gcggucuccg     2160

cagacguggg augcagcgua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7293
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 2 opt

<400>  7293
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucaucaacgc acgcaaagaa aaaaaacggc gaggcgccga caccagcgua ggcauaguag      120

gacuccuacu aacaacagcc auggccgccg aaguaacccg acgcggcucc gccuacuaca      180

uguaucucga ccggaacgac gccggcgaag caauauccuu ccccacaacc cucggcauga      240

acaaaugcua cauacaaauc auggaccucg gccauaugug cgacgcaaca augucauacg      300

aaugccccau gcucgacgaa ggcguugagc cagacgacgu cgacuguugg ugcaacacca      360

caagcaccug gguagucuac ggaacaugcc accauaaaaa aggcgaagcc agacgaucca      420

ggagagcggu aacucugcca ucacacucca cacgaaaacu acaaaccaga ucccaaacau      480

ggcucgaaag ccgcgaauac accaagcacc ucauccgcgu agagaacugg auuuuccgaa      540

accccggauu cgcccuagcc gccgccgcca ucgccuggcu ucugggaucc ucgaccagcc      600

aaaaaguaau cuaccucguc augauccucu uaauagcccc cgcguacagc auacgaugca      660

ucggaguauc gaaccgcgac uucguagagg gaaugagcgg cggcacaugg guagacguag      720

uacucgagca cggagguugc guaacaguca uggcacaaga caaaccuaca guggacaucg      780

aacucguaac gaccaccguc agcaacaugg cggaagugcg cucauacugc uacgaagcau      840

cgauaagcga cauggccucc gacucccgcu gcccaacgca aggcgaagcc uaccucgaua      900

aacaguccga cacccaguac gucugcaaac gcaccuuagu cgacagggga uggggaaacg      960

gcugcggccu auucggcaaa ggcagccuug ucaccugcgc caaauucgcg ugcagcaaaa     1020

aaaugacagg aaaauccauc caacccgaaa acuuagagua ccgcauaaug uuaucagucc     1080

acggauccca acacucagga augaucguca acgauacagg ccacgaaaca gacgaaaacc     1140

gcgccaaagu cgaaaucaca cccaacuccc cccgcgcaga agccacccua ggcggguucg     1200

gaagccucgg acuagacugc gagccccgca caggacuuga cuucagugac cuauacuacc     1260

uaacaaugaa caacaagcac uggcucgucc acaaagaaug guuccacgac aucccccuac     1320

ccuggcacgc aggagccgau accggcacac cucacuggaa uaacaaagaa gcgcuagucg     1380

aauucaaaga agcacacgca aaacgacaaa caguaguagu guuaggcuca caagaaggcg     1440

caguccacac cgcgcuagcc ggagcccuag aagcggaaau ggacggcgca aaaggccgcc     1500

uaucaagcgg ccaucuaaag ugucgacuaa aaauggacaa acuccgacug aaaggcguau     1560

ccuauagccu gugcaccgcc gcauucaccu ucacaaaaau ccccgcagag acccuucacg     1620

gaacggucac uguagaaguc caguacgcag gcaccgacgg accaugcaaa guccccgcac     1680

aaauggccgu ugacaugcaa acccuaacac cagucggaag acucauaacc gccaacccag     1740

ucaucacaga aaguaccgaa aacagcaaaa ugaugcucga acucgacccc ccguucggcg     1800

acagcuacau cgugaucggc guaggcgaaa aaaaaauaac gcaccacugg cauagaucag     1860

gcagcaccau aggaaaagcc uucgaagcaa caguacgcgg cgcaaaacga auggccguac     1920

ucggcgacac ggcaugggac uucggcuccg ugggcggagc gcugaauagu cugggcaaag     1980

guauccacca aaucuuuggg gccgcauuua aaucccucuu cggcggcaug ucaugguucu     2040

cacaaauccu aauaggaaca cuucugaugu ggcugggacu caacacgaaa aacggcucca     2100

ucucccugau gugccuagca cucggcggcg uacugauauu ccuauccaca gcagucucag     2160

cagacguagg cugcucgguc gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7294
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 2 opt

<400>  7294
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucaucaacgc aaggaaagaa aagaaaagac ggggcgcgga cacaagcguc gggaucgucg      120

gucuacuacu cacaaccgca auggccgcug aagucacccg cagaggcagc gcauauuaca      180

uguaccugga cagaaaugac gcaggagagg caaucagcuu ccccacaacg cuaggcauga      240

acaaaugcua cauacaaauc auggaccuag gacacaugug cgaugcgaca augagcuaug      300

agugccccau gcucgacgaa ggagucgaac cagacgaugu cgacugcugg ugcaacacca      360

caagcacgug ggucguauac ggcacaugcc accacaaaaa gggcgaagca cgacgaagca      420

gacgagccgu cacacugcca ucacacucca cacgcaaacu ccaaacacgg agccaaacau      480

ggcuggaauc ccgggaauac accaaacacc ucauccgggu cgaaaacugg auauucagaa      540

acccaggauu cgcccucgca gcugccgcca uagcauggcu ccuaggcuca agcacaucac      600

aaaaagugau auaccuugua augauacuac ucaucgcgcc cgccuacuca auacgaugca      660

uagggguaag caaucgcgau uucguggaag guaugucagg uggcaccugg guagacgucg      720

ugcucgagca cggaggaugu guaacgguaa uggcacagga caaacccacg gucgacaucg      780

agcucgucac cacgacggua agcaacaugg ccgaaguacg cucauacugc uacgaggcgu      840

ccauaucaga cauggcauca gacagccgcu gccccaccca gggagaggca uaccuggaca      900

aacaaaguga uacacaauac guaugcaaac ggacgcucgu cgacagaggc uggggcaacg      960

gauguggacu auucggcaaa ggaagccuag uaacgugcgc caaauucgcc ugcagcaaga     1020

aaaugacagg aaaauccaua caaccagaaa accuagaaua ccgaauaaug cucucaguac     1080

acggcucaca acacaguggc augauaguaa acgacaccgg ccaugaaacu gacgaaaacc     1140

gcgccaaagu cgaaaucacc cccaacagcc cgcgagcaga agcaacacuc ggaggauucg     1200

gauccuuagg acucgacugc gaaccacgca caggacucga uuucagcgac cucuacuacc     1260

ucacgaugaa caauaaacau uggcuagucc acaaagaaug guuccacgac aucccccuac     1320

ccuggcacgc aggcgccgac acaggaacac cccacuggaa caacaaagaa gcacugguag     1380

aguucaaaga cgcacacgcc aaacgccaga cagucguggu ccuaggauca caggaggggg     1440

caguccacac cgcacucgcc ggagcccuag aagccgagau ggacggagcc aaaggaagac     1500

ucagcucggg ccaccucaaa ugcagacuaa aaauggauaa acuaagacuc aaaggugucu     1560

ccuacucccu cuguacagcc gccuucacau ucaccaaaau accagccgag acgcuacacg     1620

gcaccguaac agucgaggug caguacgcag gcgcggacgg accaugcaag gugccagccc     1680

aaauggccgu agacaugcag acccucacac cgguaggccg acuaauaacc gccaaucccg     1740

uaauaaccga auccaccgaa aacucaaaaa ugauguuaga acuggacccg cccuucgggg     1800

acagcuacau agucaucgga gugggagaaa agaaaaucac acaccacugg cacagaagug     1860

gaagcacaau agggaaagcg uuugaggcca ccguacgcgg cgccaaacgc auggcagucc     1920

uaggagacac cgcaugggac uucggcagcg ucggcggagc acucaacuca cugggcaagg     1980

gaauccacca aauuuucggc gcagcauuca aaagccuauu cggaggaaug agcugguucu     2040

cacaaauacu gaucggcaca cuacuaaugu ggcugggacu gaauaccaaa aacggaagca     2100

ucaguuuaau gugccuugca cuaggaggcg uacugauauu ccuaagcaca gccgucuccg     2160

cagacguggg gugcagcgua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7295
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 2 opt

<400>  7295
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaauaaacgc agggaaagag aagaaacgaa ggggagccga cacaaacguc ggcaucgucg      120

gacuauugcu aacaacggcg auggcagccg aaguaacacg ccgcggauca gcguacuaca      180

uguaccugga ccgaaacgac gccggagaag caaucuccuu cccaaccacg cucggcauga      240

acaaaugcua cauccaaaua auggaccuag gacacaugug cgacgccacc auguccuacg      300

aaugcccgau gcucgacgaa ggcgucgaac cagacgaugu cgauugcugg uguaauacca      360

cgaguacaug ggucgucuac ggcacaugcc aucacaagaa aggagaagca cgcagaagca      420

gacgcgccgu aacacuacca agccauucca caagaaaacu acagacaagg agccaaacau      480

ggcuggaaag ccgcgaauac acaaaacacc uaaucagagu ggaaaacugg aucuucagga      540

auccaggauu cgcacuggcc gcggcggcaa uagcguggcu ccuaggaagc aguacaucac      600

aaaaaguaau cuaccucgua augauauugc ugaucgcgcc agccuacucu auaaggugca      660

uaggagucag caaccgugac uucguagaag gaauguccgg cggcaccugg gucgacgucg      720

ugcucgaaca cggagggugu gucaccguaa uggcccaaga caaacccacc guagacauag      780

aacuggucac aacgaccgua agcaacaugg cagaagugcg cagcuacugc uacgaggccu      840

caaucagcga cauggcaagc gacucccgcu gcccaacaca aggcgaagca uaccuggaca      900

aacagagcga cacccaguau gucugcaaaa ggacccucgu cgaccgagga uggggaaacg      960

gaugcggccu auucggcaaa ggaucccuag ugacgugcgc caaauucgca ugcaguaaga     1020

aaaugaccgg caaaagcaua caaccagaaa acuuagaaua caggaucaug cucucagugc     1080

acggaucaca acacagcgga augauaguca acgacaccgg ccacgaaacg gacgaaaaca     1140

gagccaaagu cgaaauuaca cccaacaguc cacgcgcaga agcgacccuc ggaggguucg     1200

ggagcuuagg acucgacugc gaaccacgaa cgggacucga cuucagcgac cuguauuacc     1260

uaacaaugaa caacaaacau uggcuagugc acaaagagug guuccacgac aucccccuac     1320

cauggcacgc cggggccgac acaggcacac cccauuggaa caacaaagaa gcucuagucg     1380

aguucaaaga cgcacacgca aaaaggcaaa ccgucguggu acuuggcagc caagaaggcg     1440

caguccacac ugcacuagca ggagcgcuag aagccgaaau ggacggagca aaaggccgcc     1500

uauccucggg gcaccucaaa ugcagauuga aaauggacaa acuaagacug aaaggaguau     1560

cauacucccu cugcaccgcu gccuucaccu ucaccaaaau acccgcagaa acccuccacg     1620

gaacgguaac agucgaagua caguaugccg gaaccgacgg ccccugcaag gucccagccc     1680

aaauggccgu cgacaugcaa acacuaaccc caguuggccg ccuaaucacg gccaacccag     1740

uaaucacaga aucaaccgag aacuccaaaa ugaugcucga acucgaccca cccuucggag     1800

acagcuacau cguaaucggc gucggcgaaa agaaaauaac ccaccacugg caccgcagcg     1860

gaagcacaau uggcaaagcg uucgaagcaa cggucagagg cgcacgcaga auggcaguuc     1920

ucggcgauac agcaugggau uucggcagcg uaggaggggc gcucaacucc cucgggaaag     1980

gcauacauca aaucuuuggg gcagcguuca aaagccuguu cggcggcaug ucaugguuca     2040

gucaaauccu aauaggaaca cuccuaaugu ggcucgggcu aaacacgaaa aacgggucca     2100

ucagcuuaau gugccucgca cucggaggag uacucaucuu ccuauccaca gcuguaagcg     2160

cagacgucgg augcucagug gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7296
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 2 opt

<400>  7296
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaacgc cagaaaagag aagaaacgac gaggagcaga aaccagcgug gggauagugg      120

gccugcuccu aaccacagca auggcugccg aagugacacg aagaggaagc gccuauuaca      180

uguaccugga cagaaaagac gcaggagaag ccaucagcuu uccaaccaca cucgggauga      240

acaaaugcua cauccagaua auggaccuag gccacaugug cgacgcaacc augucauacg      300

aaugccccau guuagacgaa ggcguggagc cggacgacgu agacugcugg ugcaacacca      360

ccagcacuug ggugguguac ggaacaugcc accacaagaa aggcgaagcc agacgcagca      420

gacgagccgu cacgcucccc agccacucca cacggaaacu acagacacga agccaaacau      480

ggcuagaaag ccgcgaauac accaaacacc uaaucagagu cgagaacugg aucuuccgca      540

aucccggcuu cgcauuagcc gcagccgcaa uagccuggcu acuaggcagc agcacauccc      600

aaaaaguuau cuaucuugug augauacugc uaauagcccc cgcguacuca auccggugca      660

ucggcgucuc aaaccgggac uucguagaag gcaugucagg cggcaccugg gucgacgucg      720

uacuagaaca uggaggaugc gucaccguaa uggcccaaga caaacccacc guagacaucg      780

aacucgucac gacuaccguc aguaacaugg cagaagugcg auccuacugc uacgaagcca      840

gcaucuccga cauggccucc gacagccgcu gcccaacaca aggcgaagca uaccucgaca      900

aacaaagcga cacacaauac gugugcaaac gaacccuagu cgaccgcgga ugggguaacg      960

gaugcggccu cuucggaaag ggcagccuag ugaccugcgc aaaguucgca ugcagcaaga     1020

aaaugacagg caaaaguauc cagccagaaa aucuggagua cagaauaaug cuuucaguac     1080

acggaucaca acacucagga augaucguca acgacacagg ccaugaaaca gacgagaacc     1140

gcgcgaaagu agaaauaacc cccaacagcc ccagagcaga agcaacacuc ggaggguucg     1200

gcagccuagg acucgacugc gaaccacgaa ccgggcuaga cuucagcgac cuauacuacu     1260

uaacaaugaa caauaaacac uggcucguuc acaaagaaug guuccacgac aucccccuac     1320

cauggcaugc aggagccgac accggaacgc cccacuggaa caacaaggag gccuuaguag     1380

aguucaagga cgcacacgcg aagcgacaaa ccguagucgu ccuaggaucc caagaaggag     1440

ccguacacac agcacuggcc ggcgcccucg aagcagaaau ggacggggca aaaggccgac     1500

uaagcagcgg ccaccuaaag ugcagacuga agauggacaa acugagacuc aaaggcgucu     1560

ccuacagccu cugcaccgcc gcauucacau ucaccaagau accagcggaa acacuacacg     1620

gaaccgucac cguagaagug caauacgcag gcacugacgg ccccugcaaa gucccagcac     1680

aaauggcagu agacaugcaa acacucacac caguaggacg acucaucacc gccaacccag     1740

uuaucaccga gucaacagaa aacucaaaaa ugaugcucga acucgacccg cccuucggcg     1800

acagcuacau cguaauagga gugggagaaa agaaaauaac gcaccacugg caccggagcg     1860

gcagcacaau aggaaaagca uucgaagcaa cagucagagg cgccaaaaga auggccgucc     1920

ucggcgacac ggcaugggac uucgggagcg ucggaggcgc auuaaacucc cuggggaaag     1980

gaauccacca aauuuucgga gccgccuuca aaagccuauu cggcgggaug ucaugguuca     2040

gccaaauccu cauugggacc cuacuaaugu ggcuaggccu gaacacgaag aacggaagca     2100

uaucacuaau gugccuagca cuagggggag uccuaauauu ccuauccacg gcgguaagcg     2160

cagacguagg augcuccgua gacuucagca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7297
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 2 opt

<400>  7297
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucaucaacgc gagaaaagaa aagaaaagac guggagcgga cacguccguc ggaaucgucg      120

gacuauugcu aacgacggcu auggccgcgg aagucacacg cagaggcagc gccuacuaca      180

uguaccucga ccgcaacgac gcuggcgaag cgaucagcuu ccccaccacc cuuggaauga      240

acaaguguua cauccagaua auggacuuag gacacaugug cgacgccacc auguccuacg      300

aaugcccuau gcuugacgaa ggcgucgagc cagacgacgu ggauugcugg ugcaacacaa      360

ccagcacaug gguaguauac ggaaccugcc aucacaagaa aggcgaagcc cgaaggagua      420

gacgagccgu aaccuuacca ucgcacucaa cacgaaaacu acaaacccgc ucccaaacau      480

ggcuagaauc ccgcgaauac acaaaacacc ucauccgcgu cgaaaacugg auauuccgca      540

accccggauu cgcacuagcc gccgccgcaa ucgccuggcu acucggaagc ucaaccucgc      600

aaaaagucau auaccucgua augauacugc uaauagcacc ggcauacucc auacgaugua      660

ucggcguguc caacagggac uucgucgaag gaauguccgg aggaaccugg gucgacgugg      720

uacuagagca cggaggcugc gucacaguga uggcccagga caaaccaaca gucgacauag      780

agcuagucac aacaaccgug uccaacaugg ccgaaguaag guccuacugc uacgaagcca      840

gcauaagcga caugguauca gauucgcgau gccccaccca aggcgaagca uaccucgaca      900

aacaaagcga cacccaguac guaugcaaac gcacgcucgu cgacagagga uggggcaacg      960

gaugcgguuu auucggcaaa ggaagccuag uaaccugcgc aaaauucgcc ugcaguaaga     1020

aaaugaccgg aaaauccaua caaccagaaa accuagaaua ccgcaucaug cucuccgucc     1080

acggaucaca acacagcgga augauaguca acgacacggg acacgaaacc gacgaaaaca     1140

gggccaaagu agaaauaacc ccaaacaguc cccgugcaga agcaacacuc ggcggguucg     1200

gcucacucgg ccucgacugc gaaccaagaa ccggccuaga cuucuccgac cucuacuacc     1260

uaacaaugaa caacaaacac uggcucguac acaaagaaug guuccacgac auaccccuac     1320

cauggcacgc cggcgccgac accggcacuc cacacuggaa caacaaggaa gcccucguag     1380

aguucaaaga cgcacacgcu aaacgccaaa ccgucguagu ccucggaagc caggaaggag     1440

ccguccacac cgcccuggca ggggcccugg aagcugaaau ggacggagca aaaggcagac     1500

ucucaagcgg ccaccuaaag ugccgacuca aaauggacaa acucagacua aagggaguaa     1560

gcuacagccu cugcacagcc gcauucacau ucaccaaaau cccagccgaa acucuacacg     1620

gcacagucac cguagaagua caauacgccg gaacagacgg accguguaaa guaccagcuc     1680

aaauggccgu ggacaugcaa acacugacac ccgucggaag acucauaacc gccaacccag     1740

ucauaaccga aucgaccgaa aacagcaaga ugaugcuaga acuagacccg cccuucggag     1800

acagcuacau cguaaucggc guaggggaaa agaaaauaac ccaccacugg cacagaucag     1860

gcuccacaau aggaaaagcc uucgaagcca caguacgagg cgccaaacga auggcuguau     1920

uaggugacac ggcaugggac uuuggaucag uaggcggcgc ccuaaacuca cuaggaaaag     1980

gcauccacca aaucuucggg gcagccuuca agucgcucuu ugggggcaug uccugguucu     2040

cgcaaauccu caucgggacc cuccuaaugu ggcuaggacu caacaccaag aacgguucga     2100

uaucccugau gugccucgcu cucggaggag uccucaucuu ccucuccacu gccgucagcg     2160

ccgacguugg cugcucgguc gauuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7298
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 2 opt

<400>  7298
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucauaaacgc aagaaaagaa aagaaaagaa gaggcgcgga uaccaauguc ggaaucgugg      120

gacuccuacu aacaacggca auggcagcgg aaguaacccg acgaggcagc gcauacuaca      180

uguaccucga cagaaacgac gcaggagaag caaucagcuu ccccaccacu cuaggcauga      240

acaaaugcua cauccaaaua auggaccucg ggcacaugug cgacgcgaca auguccuacg      300

aaugccccau gcuggacgaa ggcguagaac ccgacgacgu cgacugcugg ugcaacauga      360

cgagcacaug gguaguauac gggacgugcc accacaagaa aggcgaagca cgacgaagcc      420

gccgggcagu aacccuccca agccacagca cgcgcaaacu acagacucgc ucacaaaccu      480

ggcucgagag ccgagaauac acaaagcacc uaauccgcgu agaaaacugg aucuuccgaa      540

accccggcuu cgcacuagca gcagcagcga uagcguggcu acugggcuca agcaccaguc      600

agaaagucau cuaccuggua augauacuac uaauagcacc agccuacagc aucaggugca      660

uaggcguaag caaccgagac uucguagaag gaaugagcgg aggcaccugg guggacgucg      720

uucucgaaca cggagggugc gucacaguaa uggcccaaga caaacccaca guggacaucg      780

agcuaguaac aaccacaguc agcaacaugg cagaaguccg cucauacugc uacgaagccu      840

ccaucuccga cauggccagu gacucacgcu gcccaacaca aggagaagcc uaccucgaca      900

aacaaucaga cacacaguac gucugcaaac gcacacuggu cgaccgcgga uggggaaaug      960

ggugcggacu auucggaaaa ggaucccucg uaaccugcgc caaauucgcc ugcaguaaga     1020

aaaugacagg caaaucaauc cagccggaaa accuagaaua ccgcaucaug cucagcgucc     1080

acggcagcca acacagcggu augauaguaa acgacacagg acacgaaaca gaugaaaacc     1140

gcgcaaaagu cgagaucaca cccaacagcc cccgcgccga agcaacccua gguggguuug     1200

gcagccuggg acuagacugc gaaccacgaa ccggacucga cuucuccgac cuauacuacc     1260

uaacuaugaa caauaaacac uggcucguac auaaggaaug guuccacgac aucccacuac     1320

cauggcacgc uggagccgac accggaaccc cgcacuggaa caacaaagaa gcgcucguag     1380

aguucaaaga cgcacacgcc aaacgacaaa cagucguugu acucgguagc caggaaggag     1440

caguacacac agcacucgcc ggagcacuag aagcagaaau ggacggagca aaaggacgac     1500

ugagcagcgg ccaccugaag ugcagacuca aaauggacaa gcuccgccuc aaaggaguca     1560

gcuacagccu cugcaccgcc gcguucaccu ucacaaaaau accagcggaa acacuucacg     1620

gaacgguaac aguggaagua caauacgccg gaacagacgg acccugcaaa gucccagccc     1680

agauggccgu cgacaugcaa accuuaaccc cagucggccg ccuaaucacc gcaaaccccg     1740

ucaucaccga auccaccgaa aacuccaaaa ugaugcugga acuagaccca cccuuuggag     1800

acuccuacau cguuauagga guaggcgaga aaaagaucac ccaccacugg caccgcuccg     1860

gaagcacgau cggcaaagcc uucgaagcca ccguccgggg agccaaacgc auggcaguac     1920

uaggagacac agcaugggac uucggaagcg ugggcggggc ccugaacagc cuaggcaaag     1980

gaauccacca gauauucggc gccgccuuca aaagccuauu cggcggcaug uccugguucu     2040

cgcaaauacu uaucggcaca cuauuaaugu ggcucggacu caacacaaag aacggcucaa     2100

ucagccuaau gugucucgcu cugggaggag uccuaauauu ccucagcacg gcagucagcg     2160

cggacgucgg cugcucaguc gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7299
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 2 opt

<400>  7299
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaacgc ccgaaaagag aaaaagcgac gcggggccga cacgagcguc gguauaguag      120

gccuacuucu gaccacagcc auggcggccg aagucacgcg ccggggcagc gccuacuaca      180

uguaccuaga ccgaaacgac gcaggagaag caaucagcuu cccaaccacg cuaggcauga      240

acaaaugcua cauacaaauc auggaccuag gucacaugug cgacgcaacc augagcuacg      300

aaugcccuau gcuagacgaa ggagucgaac ccgacgacgu ggacugcugg ugcaacacca      360

caagcacaug ggucgucuac ggaaccugcc aucacaaaaa gggcgaagca cgcagaagcc      420

gacgcgccgu caccuugcca agccacagca cccggaaacu gcaaacaaga agccaaacgu      480

ggcuagaaag ucgcgaauau accaaacauc ucauacgcgu cgaaaacugg aucuuccgaa      540

accccggcuu cgcacucgca gcggccgcaa uagcauggcu ccuagggucc agcacaagcc      600

aaaaaguuau cuaccucgua augauccuac ucaucgcucc agcauacucc auacgaugca      660

ucggcgucag caaccgagau uucgucgaag gaauguccgg aggcaccugg guagacgucg      720

uacucgagca cggcggcugc gucaccguca uggcccaaga caaaccaacu gucgacauag      780

aacucgucac caccaccguc ucaaacaugg ccgaagugcg cagcuacugc uacgaagcga      840

gcaucagcga cauggccagc gacuccagau gucccacuca aggagaagcc uaccucgaca      900

aacaauccga cacccaauac gugugcaaac gcacgcuagu cgaccgagga uggggaaaug      960

gaugcggacu cuuuggaaaa gguucguuag uaaccugcgc caaauucgca ugcuccaaaa     1020

agaugacagg caaaucaaua cagccggaaa accucgaaua cagaaucaug cucagcgucc     1080

acggcagcca acauagcgga augaucguaa acgacacggg acacgaaaca gaugaaaaua     1140

gggcaaaagu cgagaucacc ccuaacaguc cccgagccga ggcggcacua ggcggauucg     1200

gaucacuagg acucgacugc gagccccgca cuggccugga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaaacac uggcuagugc acaaggaaug guuccacgac aucccccuac     1320

ccuggcacgc cggcgccgac accggaacuc cccauuggaa caacaaagaa gcacuggucg     1380

aauuuaaaga cgcacacgcg aaacgacaaa cagucguagu acucggaucc caagaaggug     1440

ccguccacac agcacuagcc ggagcgcugg aggcagagau ggauggcgcc aagggccggc     1500

ucuccuccgg ccaccuaaag ugucgucuaa aaauggacaa acuccgccua aaaggcgugu     1560

cguacagccu augcaccgca gccuucaccu ucaccaaaau ccccgccgag acacuacacg     1620

gaaccgucac agucgaaguc caauacgccg gcacagacgg cccgugcaaa gugccggccc     1680

aaauggcagu ggacaugcag acccuaacac cagucggacg gcuaaucaca gccaaucccg     1740

uaaucacaga aagcacagaa aacagcaaaa ugauguuaga acuagauccc ccauucggcg     1800

acagcuacau agucauaggc gucggagaga agaaaauaac acaccacugg cacagaagcg     1860

gcagcacaau cggaaaagca uuugaagcaa ccguccgcgg agcaaaaaga auggcaguac     1920

ugggcgacac cgcaugggac uucggaucag uaggcggcgc acuaaacuca uuaggcaaag     1980

ggauacacca aauauucgga gcugcauuca aaagccucuu cgggggcaug agcugguucu     2040

cacaaacacu caucggcacc cuacugaugu ggcugggacu aaauaccaaa aacggaagca     2100

ucucgcucau gugccucgcc uuaggaggag uccuaaucuu ccucagcacc gcuguaagcg     2160

ccgacgucgg cugcagcguc gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7300
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 2 opt

<400>  7300
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaaugc caggaaagaa aagaaaaggc gcggcgcaga aaccucagug ggaaucgucg      120

gacuacuacu gaccacagca auggccgccg aggugacacg acgcggaucu gccuacuaca      180

uguaccuaga ccgaaacgac gcuggcgaag cgaucuccuu ccccacaacc cucggaauga      240

auaaaugcua cauacaaaua auggaccuag gccacaugug ugacgccaca auguccuacg      300

aaugcccaau gcucgacgaa gguguggaac ccgacgacgu ggacugcugg ugcaacacaa      360

cgucaacgug gguaguauac ggaaccugcc accacaagaa aggagaagca agaagaagcc      420

gacgagcugu aacacucccg agccacagca cgcgaaaacu ccaaaccaga agccaaacgu      480

ggcuagaaag cagagaauac accaaacacc uaauacgcgu cgagaacugg auauuucgaa      540

accccggauu cgcacucgca gccgccgcaa uagccuggcu acuaggcagu ucaaccaguc      600

aaaaagucau cuaccuaguu augauacuac ucaucgcacu agcauauagc auccgaugua      660

uaggggucuc aaaccgcgac uucguagagg gaaugagcgg cggcaccugg guagacgucg      720

uccucgaaca cggcggaugc guuaccguaa uggcgcagga caaacccacc guggacauag      780

agcuaguuac cacgacagua agcaacaugg cggaagucag aucauacugc uacgaagcaa      840

gcaucagcga cauggcaagc gacagcagau gcccaacgca aggagaagcc uaccucgaca      900

aacagagcga cacacaauac gucugcaaac gcacgcuugu agaccgagga uggggcaacg      960

gaugcggacu guucggaaaa ggcucauuag ucacaugcgc caaauucgcg ugcagcaaga     1020

aaaugaccgg aaaaaguauc caacccgaaa accucgaaua ccgaaucaug cucuccgucc     1080

acggaucaca gcacagcgga augauaguaa acgacacagg acacgaaacc gaugaaaacc     1140

gggcgaaagu agaaaucacu ccaaacuccc cuagagcgga agccacacua ggcggcuucg     1200

gaagccuagg ccucgacugc gaaccacgca cgggccucga cuucagcgac cucuacuacc     1260

uuaccaugaa caacaaacac uggcugguac acaaagaaug guuccacgac aucccccuac     1320

cauggcacgc aggagcagac acaggcacac cccacuggaa caauaaagag gcacucgucg     1380

aguucaaaga cgcccacgca aaacgacaaa ccgucgucgu ccuaggaagu caagaaggcg     1440

cgguccacac ggcacucgcc ggugcgcuag aagcagagau ggacggcgcg aaaggacgac     1500

uaagcuccgg acaucucaaa ugcaggcuca aaauggacaa acuccggcug aaaggaguaa     1560

gcuacucgcu cugcaccgcu gccuucacau ucaccaaaau ccccgccgaa acacuccacg     1620

gcaccgucac cgucgaggua caauacgcag gcacagaugg accgugcaaa gucccagcac     1680

aaauggccgu agacaugcaa acacugacac ccgucggacg acucaucacc gcuaaccccg     1740

ucaucaccga aaguaccgag aacagcaaaa ugaugcuaga acucgacccg cccuucggag     1800

acucauacau cguaaucgga gucggggaaa agaaaauaac acaccauugg caccgauccg     1860

gcuccacuau aggaaaagcg uucgaagcca cgguacgcgg ugccaaacga auggcagucc     1920

ucggcgacac cgccugggac uucggaagug ucggaggcgc auuaaacucc cucggcaaag     1980

gaauacacca aaucuucggc gccgcauuca aaagccuauu cggaggaaug uccugguucu     2040

cccaaauacu gauagggaca cuccucaugu ggcucggucu aaacaccaaa aacggaagca     2100

ucucccucau gugccuugcc cuuggaggag uacucaucuu cuugucaaca gccguaagug     2160

cugacgucgg cugcagcguc gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7301
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 2 opt

<400>  7301
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucauaaacgc uaggaaagag aagaaacggc gaggagcgga cacauccgug ggaauaguug      120

gccugcuccu aacaaccgca auggcggcag aaguaacgcg ccgaggcucc gccuacuaca      180

uguaccuaga ccguaacgau gcuggggagg caauauccuu cccuaccacc cucggcauga      240

acaaaugcua cauccaaaua auggaccucg gacacaugug cgacgccaca auguccuacg      300

agugcccgau gcuagacgaa ggcgucgaac cagacgacgu cgacugcugg ugcaauacaa      360

ccagcaccug ggucguauac ggcacuugcc accacaagaa aggagaagcc agacgcagca      420

gacgagccgu cacgcuccca agccacucca cccgcaaacu acagacccga ucccaaacau      480

ggcucgaaag ccgagaauac acaaaacacu uaauccgcgu cgagaacugg aucuuccgca      540

accccggcuu cgcacucgca gcggccgcca uagccuggcu ccucgggagc agcaccuccc      600

agaagguaau cuaccuggug augauacucc uaauugcacc cgccuacucc auacggugca      660

uaggcauauc caaccgagac uucgucgaag gcaugucagg aggaaccugg gucgacgucg      720

uucuagaaca cggaggcugc guaaccguca uggcccaaga caaaccaaca gucgacauag      780

agcuagucac cacaaccguc uccaacaugg ccgaagucag aaguuacugc uacgaggcaa      840

gcaucagcga cauggccagc gacagccgcu gccccacaca aggagaagca uaccucgaua      900

aacaaucaga cacccaauac gucugcaaac gcacacuggu agacagagga uggggcaacg      960

gcugcggucu cuucgggaaa ggcucacugg uaaccugcgc caaauucgcc ugcuccaaga     1020

aaaugacagg gaaauccaua caaccagaaa accuagaaua ccgcauaaug cuaucugucc     1080

acggaucaca acacuccggc augauaguaa acgacaccgg gcacgaaacc gacgaaaaca     1140

gagccaaagu agaaaucacc cccaacagcc cccgcgcgga agcaacacuc ggaggauucg     1200

gcauacuagg acuggacugc gaacccagaa ccggacugga uuucagcgac cuuuauuacc     1260

ucacaaugaa caauaaacac uggcuggucc auaaagaaug guuccacgac auacccuuac     1320

ccuggcaugc gggagcagac acaggcacac cgcacuggaa caauaaagaa gcccucgucg     1380

aguucaaaga cgcccacgca aaaaggcaaa ccgucguagu ccugggcucc caagaaggag     1440

ccguacacac cgcacucgcc ggcgcccuag aagcagagau ggacggggcc aaaggccgac     1500

uaagcuccgg ccaccucaaa ugccgucuaa aaauggacaa acuacgcuua aagggcgugu     1560

cauacucacu cugcacagca gccuucacau ucaccaaaau accggcagaa acacugcacg     1620

gcacagucac aguggaagug caauaugccg gcacagacgg cccauguaaa gucccagccc     1680

aaauggcagu agacaugcaa acgcuaacac cagugggccg ccuaauaaca gccaaccccg     1740

ucaucacaga aaguacagaa aacucaaaaa ugaugcuaga gcuagauccc ccauuuggcg     1800

acucauacau aguaauaggc gucggcgaaa agaaaaucac ccaucacugg caccgaucag     1860

gaagcacaau uggaaaagcc uucgaagcga caguaagagg agccaaacga auggcaguac     1920

ugggagacac agccugggac uucggaagcg ugggcggagc acugaacucg cuaggaaaag     1980

gcauccacca gaucuucggc gcagccuuca agucacucuu cggcggcaug agcugguucu     2040

cccagauacu caucggcacg cuccucaugu ggcuaggacu aaacacgaaa aacgguagca     2100

ucucacugau gugccucgcc cucggaggag uccucauauu ccuaucgaca gccguaagug     2160

ccgacguggg augcuccguc gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7302
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 2 opt

<400>  7302
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucauaaacgc cagaaaagaa aagaaaaggc guggcgccga cacauccgua ggaauagucg      120

gucuacuacu caccacagcc auggcagccg aagucacaag gagaggcuca gcauacuaca      180

uguaccucga ccgcaacgau gccggagagg ccauuucauu cccaacaacc cuaggaauga      240

acaaaugcua cauccaaaua auggaccucg gccauacaug cgacgccaca auguccuacg      300

aaugcccuau gcucgacgaa ggaguagaac cggacgacgu cgacugcugg ugcaacacca      360

ccucaaccug ggucguguac ggaacaugcc accacaagaa aggagaagcc agacgcagcc      420

gccgggcagu gacccucccc ucccacucga uccgcaaacu ccaaacacgc agccaaaccu      480

gguuagaauc acgagaauac accaaacauc uaaucagggu cgaaaacugg aucuuccgaa      540

acccaggcuu cgcacucgcc gcagccgcaa uagcgugguu acucggaagc uccacaagcc      600

aaaaagucau cuaccucgua augauacuuc uaauagcacc agccuacagc auccgcugca      660

ucggagucag caaccgagac uuugucgagg gcaugagcgg cggcaccugg guggacgucg      720

uacucgaaca cggcggcugu guuacgguca uggcccagga caagcccaca gucgauauag      780

aacucgucac uacaaccguc agcaacaugg cugaaguacg aagcuacugc uacgaggccu      840

ccauaagcga cauggcaagc gacucccgcu gccccaccca aggcgaagca uaccuugaua      900

aacaauccga cacacaauau guaugcaaac gcacacucgu agacagaggg uggggaaacg      960

ggugcggacu cuucggcaaa ggaucccucg uaaccugcgc caaauucgcc ugcuccaaaa     1020

agaugaccgg aaagagcauc caacccgaaa accucgaaua ucggaucaug cucuccgucc     1080

acggauccca acauagcgga augaucguaa acgacacagg gcacgaaacc gacgaaaacc     1140

gcgcaaaagu agaaaucacc ccgaacagcc cccgcgcgga agccacccuc gggggcuucg     1200

gaagccuggg ccucgacugc gaaccccgaa caggacuaga cuucuccgac cucuacuacc     1260

uaaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auaccccuac     1320

cauggcacgc aggcgccgac acgggcacac cgcacuggaa caacaaagag gcauuagucg     1380

aauuuaagga cgcgcacgca aaacgccaga caguagucgu gcugggcuca caagaaggcg     1440

ccguacacac cgcccuugcc ggcgcacuag aagcagagau ggacggcgca aaaggcagac     1500

uauccuccgg ccaccuaaaa ugcagacuaa agauggacaa acuccggcug aaaggagugu     1560

ccuacagccu uugcaccgcu gccuucacgu ucacaaaaau ccccgccgag acccuccacg     1620

gaacgguaac aguagagguc caguacgcag gcaccgacgg accgugcaaa gucccagccc     1680

aaauggccgu cgacaugcaa acgcucaccc ccgucggccg acugauaacc gcgaacccag     1740

ucaucaccga guccaccgaa aacuccaaaa ugaugcuaga acuagaccca ccauucgggg     1800

acucauacau cgugauagga guaggcgaaa agaaaaucac ccaccacugg caucguuccg     1860

gcuccacaau cggcaaagca uucgaagcca cagucagagg cgccaaacga auggccgugc     1920

ucggagacac cgccugggac uucggaagcg uaggcggcgc ccucaacucc cucggaaaag     1980

gaauccacca aaucuucggg gccgccuuca agucccucuu cggcggaaug ucaugguucu     2040

cucagauacu caucggaacu cugcucaugu ggcuagggcu uaacgccaag aauggcucga     2100

ucucacucau gugccuagca uuaggaggag uccucauauu ccuaagcacc gcgguauccg     2160

cagacguagg gugcagcguc gacuucagua aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7303
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 2 opt

<400>  7303
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaacgc cagaaaagaa aagaaacgac gaggagccga caccucagua ggcaucguag      120

gacuacuacu cacgacagca auggcagcag agguaacccg ccgcgggagu gcauacuaca      180

uguaccuuga cagaaacgac gcgggagaag cgauauccuu cccaacaacg cucgggauga      240

acaaaugcua cauccaaaua auggaccucg ggcauaugug cgacgcaaca augucguacg      300

aaugcccaau gcuagacgaa ggcguagaac cagacgacgu cgacugcugg ugcaacacaa      360

ccagcaccug ggucguauac ggcacguguc accacaagaa aggagaagcc caacgcucca      420

ggcgagccgu cacgcucccc agccacagca cccgaaaacu ccaaacccgc agccagacau      480

ggcucgaauc aagagaauac accaaacacc uaauacgagu cgaaaacugg aucuuccgca      540

accccggauu cgcccucgca gccgcagcga uagcguggcu ccugggcagc aguaccagcc      600

agaaaguaau auaccuuguc augauacucc ucauagcccc cgccuacagu auacgcugca      660

uaggcguaag caaccgcgac uucguagaag gaaugagcgg cggaacaugg gucgacgugg      720

ucuuagaaca cggcggcugu gugacaguaa uggcacaaga caagcccacc guagacauag      780

aacuggucac aacaacagua agcaacaugg cagaaguccg cucguacugc uacgaggcuu      840

caaucuccga cauggcauca gacucaaggu gcccaacuca aggagaagca uaccucgaca      900

aacaauccga cacccaauac gucuguaaac gaacacuugu cgaccgcgga ugggggaacg      960

gaugcggccu cuucgggaaa ggaucccucg uaaccugcgc caaauucgcg ugcucgaaga     1020

aaaugacggg aaaaagcaua cagcccgaaa accuugaaua cagaauaaug cucagcgucc     1080

acggaucaca acacagcggc augauaguca acgacacagg ccacgaaacc gaugaaaacc     1140

gggccaaagu agaaaucacg ccaaacuccc cacgagccga agcgacacuc ggaggcuucg     1200

gaucccuggg gcuagacugc gaaccucgaa caggcuugga cuucagcgac cuguacuacc     1260

uaacaaugaa caacaaacac uggcugguac acaaagaaug guuccacgac aucccacucc     1320

ccuggcacgc cggagcagac accggaaccc cucacuggaa caacaaagaa gcacucgucg     1380

aguucaaaga cgcacacgcc aaaagacaaa caguaguggu ccucgggagc caagaaggag     1440

caguacacac agcccucgca ggcgcccucg aagcagagau ggacggagca aaaggacgcc     1500

ugagcucagg acaccuaaaa ugcagacuaa aaauggacaa acuccgacua aaaggcgucu     1560

cuuacucacu auguacagca gcguuuaccu ucacaaaaau cccagcggaa acauuacacg     1620

gaaccgucac cgucgaagua caauaugccg gaaccgacgg cccaugcaaa guccccgcac     1680

aaauggcagu cgacaugcaa acacucaccc ccgucggacg ccugaucaca gcgaacccag     1740

uaaucaccga gucaacggag aacagcaaaa ugaugcuaga gcuggacccg ccauuuggag     1800

auuccuacau agucaucggc gucggagaga agaaaaucac acaccacugg caucgcagcg     1860

gaagcacaau agguaaagca uucgaagcga caguccgugg agcaaaaagg auggcggucc     1920

ucggcgauac cgcaugggac uucggcagcg uaggcggagc ccuaaacagc cucggaaaag     1980

gcauacacca aaucuucggc gccgccuuca aaagccuauu cggcggaaug agcugguuca     2040

gccaaauccu aauaggcaca cuccucaugu ggcucggccu caacaccaaa aacggaagca     2100

ucagccucau gugccuagcc cucggcggcg uccuaaucuu ccugucaaca gccgugagcg     2160

cagacgucgg cugcagcgug gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7304
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 2 opt

<400>  7304
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uuaucaacgc gcgaaaagag aaaaagagac gaggcgcaga caccuccgua ggcaucgucg      120

gccuacugcu caccaccgcc auggcagcag aagucaccag aagagguuca gcguauuaca      180

uguaccucga ccgaaacgac gcaggggaag caaucagcuu cccaaccacc cuaggaauga      240

acaagugcua cauacaaauc auggaccugg gacacaugug cgacgccacc augucauacg      300

aaugcccgau gcucgacgaa ggaguagaac ccgaugacgu cgacugcugg ugcaacacca      360

ccagcaccug gguagucuac ggaaccugcc accacaagaa aggcgaagca cgacgcucgc      420

gcagagcagu cacccuccca ucacacucaa caagaaaacu acaggccaga ucacaaacau      480

ggcucgaauc ccgagaguau accaaacacc ugauccgcgu cgaaaacugg auauuccgaa      540

aucccggcuu cgcacuggcc gccgccgcca uagccuggcu gcucggcagc agcaccagcc      600

aaaaagugau cuaccucguc augauacugc ucauagcccc agccuacuca auacgcugca      660

uaggaguauc aaacagagac uucgucgagg ggaugucagg aggcaccugg guagacgucg      720

uccucgaaca cggcggaugc gugacaguca uggcccagga caaacccaca guagacaucg      780

agcuagucac aacaacaguc ucaaacaugg ccgaaguucg cucauacugu uacgaagcca      840

gcaucagcga cauggcgagc gacucacgcu gcccgaccca aggagaagcc uaccuggaca      900

aacaguccga cacgcaguac gucugcaaga gaacccuagu agacaggggc uggggcaacg      960

gcugcgggcu auucgggaaa gguagccuug uaaccugcgc caaauucgcc ugcuccaaga     1020

aaaugaccgg caaaucgauc cagcccgaaa aucuagaaua ccgcauaaug cucuccgugc     1080

acggcagcca acacucaggc augauuguaa acgacacugg ccacgagacg gacgaaaacc     1140

gcgcaaaagu cgaaaucaca ccaaacuccc cacgcgcgga ggcaacccuc ggaggcuucg     1200

gaagccuagg ccucgacugu gaaccccgca ccggccuaga cuucucagac cuuuauuacc     1260

ucacaaugaa caacaaacac uggcucguuc acaaagaaug guuucacgac aucccgcucc     1320

ccuggcacgc aggcgccgac acaggaacac cccacuggaa caacaaagaa gcccuagucg     1380

aguucaaaga cgcacacgca aaacgccaaa ccgucgucgu acucggcucg caagaaggag     1440

caguacacac cgccuuggca ggcgcccucg aagccgaaau ggacggcgcc aagggccggc     1500

ucagcuccgg ccaccucaaa ugccgccuaa aaauggacaa acuacgacua aaaggaguaa     1560

gcuacagccu augcaccgcu gcauucacuu ucacaaaaau acccgcagaa acccuacacg     1620

gcaccgucac ggucgaaguu caauacgccg gcaccgacgg acccugcaaa gucccagccc     1680

aaauggcagu agacaugcag acacucaccc cagucggaag acuaaucacc gcaaacccag     1740

uaauaacaga auccaccgaa aacagcaaaa ugaugcucga acucgacccg ccguucggag     1800

acagcuacau cguaaucggc guaggcgaaa agaaaauaac acaccacugg caccgauccg     1860

gcuccaccau cggcaaggcc uucgaagcaa ccguacgagg agccaaacga auggcaguac     1920

ucggagauac ggcuugggau uucggcagcg ucgggggagc ccuaaacuca cuaggaaaag     1980

gcauccauca aaucuucgga gcggcuuuua aaagucuauu cggaggcaug aguugguuca     2040

gccagauacu aaucggcaca cuacuaaugu ggcucggccu caacaccaaa aacggaucca     2100

ucucacuaau gugucucgcc cuaggcggag uacuaauauu ccucagcacc gccgucagcg     2160

ccgacguagg augcagugug gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7305
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 2 opt

<400>  7305
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucaucaacgc gcgcaaggag aagaaacgcc gaggcgcaga cacauccguc ggaaucguag      120

ggcuacuccu cacaaccgca auggccgcag aagugacacg acgcggaucu gcauacuaua      180

uguaccuaga cagaagcgac gcaggagaag caauuagcuu cccaacgacc cucgggauga      240

acaaaugcua cauacaaaua auggaccucg gacacaugug cgacgcaacg augucuuacg      300

aaugccccau gcuagacgaa gggguggaac ccgacgacgu cgacugcugg ugcaacacca      360

caagcacgug gguagucuac ggaaccugcc accacaagaa aggcgaagcc cgcaggagca      420

ggagagccgu aacacucccc ucccacagca cccgaaaacu gcaaacgaga ucacaaacgu      480

ggcucgaauc cagagaauau acaaaacacc uaaucagagu cgagaacugg auauucagaa      540

acccagguuu cgcccuagca gcagcagcaa uagccugguu acuaggaagc agcaccagcc      600

aaaaaguaau auaccuagua augauacucc ucauagcccc agcauacagc auacggugua      660

ucggaguaag caaccgagac uucgucgaag gaaugucggg aggaacaugg guagacguag      720

uacucgaaca cggcggcugc guaacaguca uggcccaaga caaaccaaca guagacauug      780

agcuagugac uacaacaguc agcaacaugg cggaaguccg cucguacugc uacgaagcau      840

cgaucagcga cauggcaagc gacaguagau gccccacaca gggcgaagcc uacuuagaca      900

aacaaucaga cacccaauac guauguaaaa ggacccuagu cgaucgcgga uggggcaacg      960

gaugcgggcu auuuggcaaa gguucacucg uaaccugcgc gaaauucgcg ugcaguaaga     1020

aaaugacagg caaaaguaua caaccagaaa accucgagua ccgcauaaug cuaucggucc     1080

acggcuccca acacagcggc augaucguca acgacacggg acacgaaacc gacgaaaaca     1140

gagcaaaagu cgagaucaca ccaaacucac cacgagcaga ggcaacccuu ggcggauucg     1200

gcagucucgg acucgacugc gaaccgcgga caggccucga cuucucagac cuauauuacc     1260

uaaccaugaa caacaaacac uggcuaguac acaaagaaug guuccacgac auaccacuac     1320

cauggcacgc cggcgccgac accggcacac cacacuggaa caacaaagaa gcacucguag     1380

aguucaaaga cgcgcacgcg aaacgacaaa caguaguagu acuaggcuca caagaaggag     1440

caguccacac agcacuggcc ggcgcacuag aagccgaaau ggacggcgca aaaggacguc     1500

uaaguagcgg ucaccuaaaa ugccgacuaa aaauggauaa acuacgccua aaaggggucu     1560

cauacucgcu cugcaccgca gcauucaccu uuacgaaaau cccagccgaa acccugcacg     1620

ggacaguaac cguagaaguc caauacgccg gcaccgacgg cccaugcaaa guaccagccc     1680

aaauggccgu cgacaugcaa acccugaccc cggugggaag acugaucaca gccaacccag     1740

ucaucacaga aagcacggag aacagcaaaa ugaugcugga acuugaccca cccuucggcg     1800

acagcuacau cguaauaggg gugggagaaa agaaaauaac acaucacugg caccgcucag     1860

guagcacaau cggcaaagca uucgaggcaa cagucagagg agcaaaacgg auggcagucc     1920

uaggagacac agccugggau uucggaagcg uaggaggggc gcucaauuca cuaggcaagg     1980

gcauccacca aauauucgga gccgccuuca agucacuguu cggcggaaug agcugguuca     2040

gccaaauccu caucggcaca cuauuaaugu gguuaggacu gaacacuaaa aacggaucca     2100

ucagccucau gugccucgcg cugggcggcg uacugauauu ccugagcaca gccguaagcg     2160

ccgacgucgg gugcucagua gacuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7306
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 2 opt

<400>  7306
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaauaaacgc cagaaaagaa aagaagagaa ggggaacaga cacauccgug ggcaucgucg      120

gccuccugcu cacaacagcc auggcagccg aagucacccg aagaggcagc gcauacuaca      180

uguaccuaga caggucagac gcaggcgaag caauauccuu cccaacaaca cuaggaauga      240

acaaaugcua cauccagaua auggacuuag gacacaugug cgacgcaacg augucauacg      300

aaugccccau guuagacgaa ggaguugaac cagacgacgu ggacugcugg ugcaacacua      360

caucaacgug ggugguguac ggaacaugcc accacaagaa aggagaagcg cgcagaucca      420

gacgagcagu gacccuacca agccauagca cccgaaaacu ccaaacaaga agccaaaccu      480

ggcuggagag cagggaauau accaaacacc ucauaagggu cgagaacugg auauucagaa      540

acccaggcuu cgcacucgcc gcagcagcca uagccuggcu ccuaggcuca agcacaagcc      600

aaaaaguaau auaucucgug augauccuac uaaucgcacc agcauacuca auacgcugca      660

ucggcgucag uaacagggac uucguagaag ggaugucggg aggcacaugg guggacguug      720

uacucgagca cggaggaugc gucaccguca uggcacaaga uaaacccaca guggacaucg      780

aacucguaac uaccacagug ucuaacaugg cagaaguacg aucauacugc uacgaagcgu      840

cgauaucaga cauggccagu gacucccggu gcccgacaca aggagaagca uaccucgaca      900

aacagagcga cacacaauac guauguaaaa gaacgcuggu agaccgcgga uggggcaacg      960

gcugcggacu cuucggcaaa ggaucccucg uaaccugcgc aaaauucgcg ugcucaaaga     1020

aaaugaccgg aaagagcaua caaccagaaa accucgagua ccgcauaaug cucucagucc     1080

acggaagcca gcacagcggg augauaguca acgacacagg ccacgaaaca gacgaaaauc     1140

gagcaaaggu agaaaucaca ccgaacagcc cccgcgccga agccacgcua ggaggauucg     1200

gcucccuagg acucgacugu gaaccgcgca caggacuaga cuucuccgac cuauacuacc     1260

ucacaaugaa caacaaacac uggcuagugc acaaggaaug guuccaugac auaccccucc     1320

ccuggcacac cggagccgac accggaacac cacacuggaa caacaaagaa gcccugguag     1380

aguucaaaga cgcacacgca aaaagacaaa caguaguggu ccuaggcagu caagaaggcg     1440

cuguacacac agcacuagca ggagcacuag aagccgaaau ggacggcgca aaaggaagac     1500

ugucgucagg acaccucaag ugccgauuaa aaauggacaa acuaagacug aaaggagucu     1560

cguacagccu cuguaccgcg gccuucacau ucaccaagau accagccgaa acacugcacg     1620

gaacaguaac aguagaagug caguacgccg ggacggacgg accaugcaaa gugccagcac     1680

aaauggccgu agacaugcaa acacucacgc cagucggccg ccugaucacc gccaaccccg     1740

ucauaacaga agggaccgag aacucgaaaa ugaugcugga guuggaccca cccuucggag     1800

acagcuacau cguaaucgga gugggcgaga aaaagauaac gcaccacugg caccgcagug     1860

gaagcaccau cggcaaagcc uucgaagcca ccguccgcgg cgcaaagaga auggcagucc     1920

uaggcgacac agcaugggac uucggaagcg uaggaggcgu ucugaacagc cuaggcaaag     1980

gaauccacca aaucuucggc gcagccuuca aaucacucuu cgggggcaug ucaugguuca     2040

gccaaauacu aauaggcacc cuacucaugu ggcucgggcu aaacacuaaa aacggaagca     2100

ucagccuaau gugccucgcc cugggaggcg uacuaaucuu ccuaagcaca gcaguuucag     2160

ccgacguggg augcagcguc gacuucucaa agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7307
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 2 opt

<400>  7307
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauuaacgc uagaaaagaa aagaaaagac gaggagccga uacauccgua gggauugucg      120

gacuacuccu aacaacagcg auggcggcag aagugacacg gcguggcagc gccuacuaca      180

uguaccugga ccgaaacgac gcaggcgaag ccaucucauu cccaacaaca cuuggcauga      240

auaaaugcua cauccaaaua auggaucuag gccacaugug cgacgcgaca augagcuacg      300

aaugcccaau gcuagacgaa ggcgucgaac ccgacgacgu ggacugcugg ugcaacacca      360

ccuccaccug ggucguguac gggaccugcc accacaagaa aggagaagcc cgacgcucaa      420

gacgcgcggu aacacuacca ucacacucaa cccgaaaacu gcaaacccgc agucagaccu      480

ggcucgaguc gcgagaauac acaaaacacc ugauacgagu agaaaauugg aucuuccgaa      540

acccgggauu cgcccucgcc gccgccgcaa ucgccuggcu ccuaggaucc uccacaagcc      600

aaaaagucau cuaccucgua augauccucc uaaucgcacc agccuacagc auaagaugca      660

uaggaguaag caauagagac uucgucgaag gaaugucagg cggcacaugg guagacgucg      720

uacuagagca cggaggaugc gucaccgcaa uggcccaaga caaaccaaca guagacaucg      780

aauuagucac aacaacagug uccaacaugg cagaaguacg cucauacugc uacgaagcca      840

gcaucagcga cauggcgagc gacucccggu gccccacgca aggcgaagcc uaucucgaca      900

aacagucaga cacccaauac gucugcaaac gaacuuuagu ugaccgcggc uggggcaacg      960

gaugcggccu cuucggcaaa ggaucacucg ucacgugcgc gaaauucgcc ugcagcaaga     1020

aaaugacagg gaaaucaauu cagccugaaa accuagaaua ccgcaucaug uuaagcgucc     1080

acggaucaca gcacuccggc augcucguga augauacagg ccacgaaacc gacgaaaaua     1140

gagcgaaagu cgaaaucacc ccgaacucuc cacgggcaga agcaacacua ggcggcuucg     1200

gcucccucgg acucgauugc gagccccgca ccggacucga cuuuagugac cucuacuacc     1260

ucacaaugaa caacaaacac uggcucgcac acaaagaaug guuccacgac aucccgcuac     1320

ccuggcacgc cggagcagcc accggcacac cccauuggaa uaacaaagaa gcacucgucg     1380

aguucaaaga cgcacacgcg aaacgccaaa cagucguagu cuuaggaagu caggaaggcg     1440

ccguccacac cgcucucgcc ggcgcccucg aagccgaaau ggacggcgcc aaaggccgac     1500

ucuccagcgg gcaccuaaaa ugccgacuca aaauggacaa acucagacuc aaaggcguau     1560

cauacagccu cugcacagca gcauucaccu ucacaaaaau ccccgcugaa accguugacg     1620

gcacaguaac cguagaaggc caauacgggg gcaccgacgg ccccugcaaa guccccgcgc     1680

aaauggcggu agacaugcaa acccucacgc ccguaggccg ccucauaaca gccaaccccg     1740

ugaucacaga aucaacggaa aacagcaaaa ugaugcuaga acuggauccc ccauucggag     1800

acucauacau agugaucggu gucggagaaa agaaaaucac ccaccacugg caccgcucag     1860

gcucaacaau uggaaaagcc uucgaagcaa cagucagagg cgcaaaacgc auggccgucc     1920

ugggagacac agccugggac uucggcucag ugggcggcgc ccucaacucc cucgggaaag     1980

gcauccacca gaucaucgga gcggccuuca aaagccuauu cggcggaaug ucaugguuca     2040

gccaaauacu aauaggaaca cuacuaaugu ggcuaggacu caacaccaaa aacggcagua     2100

ucucacucau gugccuagcc cucggcggag ucuuaaucuu ccucucaaca gcuguaucag     2160

gcgggguagg cugcuccgua gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7308
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 2 opt

<400>  7308
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucauaaacgc acgcaaagaa aagaaacgac gagggacgga caccuccguc ggaaucguag      120

gccuccuucu gaccaccgca auggccgccg aagucacccg ccgaggcucu gcauacuaca      180

uguaccucga ccgcagcgau gccggagaag cgaucucauu ccccacaaca cucggcauga      240

acaaaugcua cauucaaauc auggaccucg gacacaugug cgacgccaca auguccuacg      300

aaugccccau gcuagaugaa ggcgucgaac cagacgaugu cgacugcugg ugcaauacaa      360

ccagcaccug ggucguguac ggcacaugcc accacaagaa aggggaagcc agacgcagcc      420

gacgagcagu cacacuaccg ucacacagca cacguaaacu ccaaacccgc agccaaacau      480

ggcuggaguc cagagaguac accaaacacc ugauaagggu agaaaacugg auauuccgca      540

acccuggcuu cgcccucgcc gccgcagcaa ucgccuggcu acucggcuca ucgaccuccc      600

aaaaaguaau cuaccucguc augauacuuc ucaucgcccc cgcauacucc auccgaugca      660

uaggaguguc caaccgcgac uucguggaag gcauguccgg cggaaccugg gucgacguag      720

uacuagaaca cggaggaugc guaacgguca uggcacaaga caaacccaca guugauauag      780

agcucguaac caccaccguc agcaacaugg ccgaaguacg cucauacugc uacgaagccu      840

ccaucucgga cauggcuagc gauucccgcu gccccacaca aggagaagcg uaccuggaca      900

aacaaucgga cacacaauac guaugcaaac ggacccuagu cgaccgcggu uggggcaacg      960

gcugcggacu uuucggaaaa ggaagccucg uaacaugcgc caaauucgca ugcucgaaaa     1020

agaugaccgg caaaagcaua caaccagaga accucgagua cagaauaaug cucucagucc     1080

acggcuccca gcacuccggc augauuguga augacacggg ccacgaaacc gacgagaauc     1140

gcgcuaaggu cgaaaucaca cccaauucac cacgcgcgga ggccacacuc ggcggguuug     1200

gaagccucgg ccucgacugc gaacccagaa caggacuaga cuucagcgac cuguacuacc     1260

ucaccaugaa caauaaacac uggcuagucc acaaagaaug guuccacgac auccccuuac     1320

cauggcaugc aggcgccgac accggcacac cccacuggaa caacaaagaa gcccucguag     1380

aguucaaaga cgcacacgcc aaacgacaaa caguaguggu acugggaucu caggaaggag     1440

ccguacacac agcccuagca ggcgcauuag aagccgaaau ggacggagcc aaaggucgcc     1500

ucucuagcgg acaucucaag ugccgccuga aaauggacaa acucagacua aagggagucu     1560

cuuacucauu augcaccgca gccuucacau uuuccaagau cccagccgaa acacugcacg     1620

gcaccgucac cgaggaagua caguacgccg gaaccgacgg cccaugcaag guaccagccc     1680

aaauggcggu cgacaugcaa acacuaaccc ccgucggccg acucauuaca gcuaaccccg     1740

uuaucacaga aucaaccgaa aacucgaaaa ugaugcuaga acuagaccca cccuucggag     1800

acuccuacau cgucaucggc guaggcgaaa agaaaauuac acaccacugg caccgcagug     1860

gcagcacgau aggaaaagca uucgaagcaa cuguccgcgg agccaaacgc auggcagucc     1920

ucggggacac cgcaugggac uucggaagcg ucggaggcgc ccucaacucg cucggaaaag     1980

gaauccacca aauuuucgga gcagcguuca agucccuguu cgggggcaug agcugguuca     2040

gccaaauacu cauaggaaca cuacucaugu gguuaggcuu aaacacuaag aauggcagca     2100

ucagccucau gugccucgcc cuaggcggag uacucaucuu ccucagcaca gccgugagcg     2160

ccgacguagg cugcagcguc gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7309
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 2 opt

<400>  7309
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaacgc ccggaaagaa aagaaacgac gaggcacuga caccuccguc ggaauagucg      120

gacuacuacu aacuacagcc auggccgcag aagucacaag gcgcggauca gcauacuaca      180

uguaccuaga ccgcucagac gccggcgaag ccaucagcuu cccaaccaca cuaggaauga      240

acaaaugcua cauacagaua auggaccugg gacacaugug cgacgccaca augagcuacg      300

aaugcccaau gcuagacgaa ggcguugaac ccgacgacgu agacugcugg uguaacacga      360

ccagcaccug ggucguauac ggaacaugcc accacaagaa aggcgaagcu aggcgcuccc      420

gacgcgccgu cacccuaccg agccauucca ccagaaaacu ccaaacgagg ucccagaccu      480

ggcuggaauc ccgagaauac acaaaacacc uuauacgagu agaaaacugg auauuccgaa      540

acccgggguu cgcccuggcc gccgcugcca uagccuggcu ccucggcucc uccacaagcc      600

aaaaaguaau auaccuggua augauccuac uaauagcccc cgccuacagc aucaggugca      660

ucggcgucuc aaacagagac uucgucgaag gcauguccgg cggaaccugg guggacguag      720

uccuggagca uggcggaugc gugacaguaa uggcccaaga caagccaacc guagacaucg      780

aacugguaac uacaaccguc agcaacaugg ccgaaguccg aagcuacugc uacgaagcca      840

gcauaagcga cauggccucc gacucccggu gcccaacaca aggcgaagcc uaccuagaca      900

aacaauccga cacgcaauac guaugcaaac gaacccuagu cgacagagga uggggcaacg      960

gcugcggccu cuucggcaaa ggaucacucg ucacgugcgc caaguucgcc ugcaguaaga     1020

aaaugacagg caaaucgaua caacccgaaa accuagaaua ccgcaucaug cuaagcguac     1080

acggcagcca acacucagga augaucguga acgacacagg ccacgaaaca gacgaaaauc     1140

gagcaaaagu agaaaucacc ccuaacagcc cacgugccga agcaacacua ggaggauucg     1200

gcagccuagg ccuugacugu gagccacgaa ccggacucga cuucuccgac cuguacuacc     1260

ucacgaugaa uaacaaacau ugguuagucc acaaagaaug guuccacgac auaccauugc     1320

cauggcacgc aggcgcggau accggaacgc cacacuggaa caacaaagaa gcacucgucg     1380

aguucaaaga ugcacacgca aaaagacaga ccguagucgu acuuggcagu caagaaggcg     1440

ccguccacac cgcgcuugcc ggagcccuag aagccgaaau ggacggcgcc aaaggccgac     1500

ucuccuccgg acaccucaaa ugccgacuga aaauggacaa acuccgacuc aaaggcguaa     1560

gcuacagcuu gugcaccgcc gccuucacau ucacaaaaau accagcagaa acucuccacg     1620

gcaccguaac cguagaagua caguacgccg ggacagacgg ccccugcaag guaccagcac     1680

aaauggccgu cgacaugcaa acccuaacac ccguuggcag acuaaucaca gcgaacccag     1740

uaaucaccga aagcaccgaa aauagcaaga ugaugcucga acucgauccc ccauucggcg     1800

acagcuacau agucauagga gucggcgaga agaaaaucac ccaccacugg caccgaagcg     1860

gcagcaccau aggaaaagcc uucgaagcca cagugagagg cgcgaaaaga auggcagucc     1920

ucggcgacac agcgugggac uucggcagcg ucggcggcgc acucaacuca cucggcaaag     1980

gcauccacca aaucuucggc gcagccuuca aaagccucuu cggcggcaug uccugguuca     2040

gccaaauacu aaucgggacc cuacugaugu ggcuaggacu aaacaccaaa aacggaucga     2100

ucucccucau gugcuuagca cucggcggcg uccuaauauu cuuaagcacc gccguauccg     2160

cagacguggg cugcaguguc gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7310
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 2 opt

<400>  7310
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaaucaacgc acgcaaagag aaaaagagaa gaggaacaga cacaucaguc ggcaucgucg      120

gauuacugcu cacgacggca auggcgguag aggugacccg caggggaaac gccuacuaca      180

uguaccuaga cagaagcgac gcaggagagg cuaucuccuu cccgaccaca augggcauga      240

acaaaugcua cauccaaauc auggaccuag ggcauaugug cgacgcaacc augagcuacg      300

aaugcccaau guuagacgag ggagucgaac cagacgacgu ugacuguugg ugcaauacca      360

caagcaccug ggucguauac ggcacaugcc accacaagaa aggggaagcg aggcgcaguc      420

gacgcgccgu cacccuaccc agccauucca cacgcaaguu acaaacgcgc agccaaacgu      480

ggcuagaaag ccgagaauac accaaacacu uaaucagagu cgaaaacugg aucuucagaa      540

acccgggauu cgcacuagca gccgccgcga ucgccuggcu acuagguucg agcacaagcc      600

aaaaagucau cuaccuagug augauacucc uaauagcccc cgcauacagc auacggugua      660

uaggcguauc aaacagagau uucgucgagg gaauguccgg cggaacaugg guagacguag      720

uacuggaaca cggcggaugc guaacaguca uggcacagga caaacccaca gucgacauag      780

aacucgucac caccaccguc agcaacaugg ccgaaguacg cagcuacugc uacgaggcau      840

caauauccga cauggcaucc gacagcagau gcccaacgca gggagaggcc uaccuagaca      900

aacaauccga cacacaguac gugugcaaaa gaacccuagu agaccgaggu uggggcaacg      960

gcugcggacu auucgggaaa ggcucgcuag uaaccugcgc caaauucgcc ugcagcaaga     1020

aaaugaccgg caaaucaauu caaccagaaa accucgaaua ccgaaucaug cucagcgucc     1080

acggaagcca acacagcgga augauaguaa acgacaccgg ccacgaaaca gacgagaaca     1140

gagcgaaagu cgaaauaaca cccaacaguc cccgcgcaga agccacccug ggaggauucg     1200

gcagccucgg ccuagacugc gaaccacgaa caggacuaga cuucagcgac cuguacuacc     1260

ugacaaugaa caacaaacau uggcuaguac acaaagaaug guuccacgac auaccacucc     1320

cuuggcacgc uggcgccgac acaggaacac cccacuggaa caacaaagaa gcccuagucg     1380

aguucaaaga ugcacacgca aaacgccaaa ccguaguggu ccugggcuca caggaaggag     1440

ccgugcacac agccuuagcg ggcgcgcucg aagccgaaau ggacggagcc aaaggcagau     1500

uaagcagcgg acaccucaaa ugccggcuga aaauggacaa acugcgacuc aaaggaguau     1560

ccuacagccu cuguacagcg gccuuuacau ucacaaaaau accagcggaa acgcuucacg     1620

ggacaguaac aguugaagua caauacgcug gaacagacgg acccugcaaa guaccggcac     1680

agauggccgu cgacaugcaa acccuaaccc caguaggacg ccugauaacg gccaacccag     1740

ugaucaccga aagcaccgaa aacucaaaaa ugaugcucga acucgacccg ccauucggcg     1800

acagcuacau agucauagga gugggagaaa agaaaauaac gcaccacugg caccgcagcg     1860

gcaguacaau cggaaaagcc uucgaagcaa ccgugcgcgg cgcaaaaaga auggccguac     1920

uaggagacac cgcuugggac uucggcagcg uaggcggagc ccucaacagc cucgggaagg     1980

ggauccacca aaucuucgga gcagccuuca agagccuuuu cggaggcaug uccugguuca     2040

gccagauacu aauagggacc cuacugguau ggcuaggucu caacaccaaa aacggcagca     2100

uaagucuuau gugccucgca cuaggcggag uacuaauauu ccuaagcaca gcagugagug     2160

ccgacguagg cugcagcgua gacuucucca agugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7311
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 2 opt

<400>  7311
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucauaaacgc ccgcaaagaa aagaaaagga gaggcacaga cacaagcgua ggaaucgugg      120

ggcuccugcu aacaacagcc auggcggccg aggucacccg uagaggcagc gcguacuaca      180

uguaccuaga ccgcagcgac gccggagagg ccaucagcuu cccgacaacc cuaggcauga      240

acaaaugcua cauacaaaua auggaccucg gccacaugug cgacgcgacc augucauacg      300

aaugcccaau gcuggacgaa ggagucgaac ccgacgacgu agacugcugg ugcaacacaa      360

cgagcacgug gguagucuac ggaaccugcc accacaagaa aggcgaagca cgacgauccc      420

gacgagcggu cacgcuccca agccacucca cccgaaaacu ccagaccaga agccaaaccu      480

ggcuagaauc cagggaauac accaaacacc ucauccgcgu agaaaacugg auauuccgaa      540

accccggcuu cgcacucgca gcagcagcca uagcauggcu acucggcagc agcacaagcc      600

aaaaaguaau auaccuggug augauacuac ugaucgcccc cgccuacagc auccgcugca      660

uuggagucag uaacagagac uucguagagg gcaugagugg aggaacaugg guggacgucg      720

uccuggaaca cggaggaugc gucacaguaa uggcacaaga caaacccaca gucgacaucg      780

aacucguuac aacgaccguc ucaaacaugg ccgaaguacg cagcuacugc uacgaggcaa      840

guauaagcga cauggcaagc gacagccgau gcccgaccca aggagaagca uaccuggaua      900

aacaaagcga cacgcaauac gucugcaaaa gaacccuagu cgaccgcgga ugggggaacg      960

gaugcggacu auucggaaaa gggagccucg uaacaugcgc aaaauucgcg ugcucaaaga     1020

aaaugacagg caaaagcauc caaccagaaa accuagaaua ccgaaucaug cuaagcguac     1080

acggauccca acacagcgga augauaguca augacacggg acaugaaaca gacgaaaaca     1140

gagccaaagu cgaaauaaca ccaaacaguc cccgcgcgga agccacccuc gggggauucg     1200

gaagccucgg cuuagacugc gagccgcgca caggacuaga cuucagcgac cuauacuacu     1260

uaaccaugaa uaacaaacau uggcucgucc acaaagagug guuccacgac aucccacuuc     1320

cguggcacgc cggcgcggac acaggcacac cgcacuggaa caacaaagaa gcccucgugg     1380

aguucaaaga cgcccacgcc aaacgacaaa cagucguagu ucuagggucc caagaaggcg     1440

caguacacac cgcacucgca ggcgcccucg aggccgaaau ggacggcgcc aaaggccgac     1500

uguccagcgg acaccucaag ugcagacuaa aaauggacaa gcuccgccua aaaggcguau     1560

cauacucgcu auguacagcg gcauucacau ucaccaaaau cccagccgaa acccuccacg     1620

gaaccguaac aguagaagua caauacgcag gaacagacgg cccgugcaaa guaccggccc     1680

aaauggcagu agacaugcaa acacucacac ccgucggaag gcuaaucaca gcaaacccag     1740

ucauaacgga aagcacagaa aacagcaaaa ugaugcucga acuagaccca ccguucggcg     1800

acagcuacau aguuauaggg guaggagaaa agaaaauaac acaccauugg cauagauccg     1860

gcagcacaau cgggaaagca uucgaagcga cagucagagg agcuaaacga auggccguac     1920

ucggcgacac agccugggac uucggauccg ugggcggggc ccugaacagu cucggcaaag     1980

gaauccacca aaucuucgga gccgcauuca aaucgcuauu cggcggcaug uccugguucu     2040

cccagauccu aaucggaacc cuauuaaugu ggcucgggcu aaacaccaaa aacggaucca     2100

uuucccuggu augccucgcc cucggaggag uacucauauu ccugagcaca gccgucagcg     2160

ccgauguagg augcuccgug gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7312
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 2 opt

<400>  7312
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaacgc aagaaaagaa aagaaacgcc gaggcacaga caccagcgug ggcauagucg      120

gacuacuccu aacaaccgcc auggccguug aagucacacg acgaggaagc gcauacuaca      180

uguaccuaga ccguagcgac gccggagaag caauauccuu cccaaccacc cuaggaauga      240

acaaaugcua cauccaaaua auggaccuag gccauaugug cgacgcuacg augaguuacg      300

aaugccccau gcuggacgaa ggcguagagc cggacgacgu cgacugcugg ugcaacacca      360

ccagcaccug gguagucuac ggaaccugcc accacaagaa aggcgaggca cgcagaagcc      420

gccgagcagu cacccuacca ucccacucaa cacgcaaacu acaaacaaga ucccaaaccu      480

ggcuggaaag ccgcgaauac acaaaacacc ugauacgagu cgagaacugg aucuuccgaa      540

acccaggcuu cgcccucgcc gcagccgcca ucgcguggcu acucggcagc uccacuagcc      600

aaaaagugau auacuuagua augauacucc ucaucgcccc agccuacucg auccgcugca      660

ucggcgucag caauagggau uuuguagaag gcaugagcgg cgguacuugg gucgacgucg      720

ugcuagagca cggcggcugc gucacaguaa uggcacaaga caaacccgcc gucgacaucg      780

aacucguaac gacgacagua ucaaacaugg ccgaagucag aucguauugc uacgaagccu      840

ccaucagcga cauggccagc gacucccgcu gcccaacaca gggagaagca uacuuagaca      900

aacaaagcga cacacaauac guaugcaaac gaacccuagu cgaccgcgga ugggggaacg      960

ggugcggucu auucggaaaa ggaucacucg uaaccugcgc caaauucgcg ugcagcaaga     1020

aaaugaccgg caaaucgaua caaccagaaa accucgaaua cagaaucaug cuauccgucc     1080

acggcuccca acacucuggc augaucguaa acgauaccgg ccacgaaacc gacgaaaacc     1140

gagccaaagu ggaaaucacc ccgaacagcc cucgggcaga agccacgcug ggcggguucg     1200

ggagccuagg acucgacugu gaaccacgca ccggacuaga uuucagcgac cuauacuacc     1260

ugaccaugaa caacaaacac ugguuagucc acaaagaaug guuccacgac auaccacucc     1320

cauggcacgc aggcgccgac acaggcaccc cacacuggaa caacaaagaa gcacugguag     1380

aguucaaaga cgcccacgcc aaacgccaaa cggucguagu gcuaggguca caggaaggcg     1440

cgguccacac cgcccucgcc ggcgcccugg aagcagaaau ggacggugcc aaaggacgcc     1500

ucagcucagg acaccuaaaa ugccgacuca aaauggacaa acugcgccuc aaaggggucu     1560

cauacucacu augcaccgcc gccuucaccu ucaccaaaau cccagccgaa acacuccacg     1620

gaacaguaac cguggaaguc caguacgccg gcaccgacgg acccugcaag gugccagcac     1680

aaauggccgu agacaugcaa acccucaccc cagucggacg ccuaauaacu gccaaccccg     1740

ucaucaccga aagcacagaa aacucaaaaa ugaugcuuga acuggauccc ccauuuggcg     1800

acagcuacau cgucaucggg guuggcgaaa agaaaaucac acaccacugg caucgcucag     1860

gcuccaccau cggcaaagcg uucgaagcca ccguccgcgg cgcaaaacgc auggccguac     1920

ugggagacac cgcaugggac uuuggaagug ucggaggcgc ccugaacuca cuagggaaag     1980

gaauacacca aaucuucgga gcagcguuca aaagccucuu cggcggaaug aguugguuca     2040

gccaaauccu uauaggaacc cuccucguau ggcucggguu aaacaccaaa aacgggagca     2100

uuagccuuac augccuagcc cucggcggcg uacucauauu ccucagcacc gcugucucgg     2160

ccgacgucgg cuguuccgua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7313
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 2 opt

<400>  7313
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgga       60

ucaucaacgc cagaaaagaa aagaaacggc gcggagcgga cacaucaguc gggaucguag      120

gccuacuucu caccacggca auggcagucg aaguaacaag acgcggaagc gccuacuaua      180

uguaccucga ccgcuccgac gccggagaag caaucagcuu ccccacuacc cuaggcauga      240

acaaaugcua cauccaaauc auggaccucg gacacaugug ugacgccacg augucauacg      300

aaugccccau gcucgacgaa ggaguggaac ccgacgacgu agacugcugg ugcaacacca      360

caucaacaug gguugucuac ggaaccugcc accacaagaa aggagaagcc cgucgaagcc      420

ggcgagccgu aacacucccu ucgcacucaa cccgaaaacu gcaaacaaga ucccaaacau      480

ggcuagaaag ucgugaauac acaaaacauc uaauacgugu ggagaacugg aucuuccgaa      540

accccggauu cgcauuagca gccgccguca uagccuggcu gcuaggaagc agcaccagcc      600

aaaaaguaau auaccugguc augauccuac ucauagcgcc agccuacucc aucagaugua      660

uaggcgucuc aaaccgggac uucgucgagg gaauguccgg aggcacuugg gucgacgugg      720

uacuggaaca cggcggaugc gucacaguca uggcacaaga caaaccaacu guagacauag      780

aacucgucac gaccacgguc agcaacaugg ccgaggucag aagcuacugc uacgaagcca      840

gcauuucaga cauggcaagc gacagccggu gcccgaccca aggagaagcg uaccuagaca      900

agcaaagcga cacccaauac gucugcaaaa gaacccucgu cgaccggggc uggggcaacg      960

gcugcggacu cuucggcaaa ggaagucuag uaacuugcgc aaaauuugcg ugcucgaaga     1020

aaaugaccgg caaaucaauc caacccgaga accuagagua cagaauaaug cuaucaguuc     1080

acggcaguca gcacuccgga augaucguaa acgacaccgg ccacgaaacc gacgaaaacc     1140

gcgcaaaagu cgaaauaaca cccaacagcc cccgagccga agccacacuc ggaggguucg     1200

gcucccuagg acuagacugc gagccacgca ccggacucga cuucucagac cuauacuacc     1260

uaaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auaccgcucc     1320

ccuggcacgc cggagcggac accggcacac cccacuggaa caacaaagaa gcccuagucg     1380

aguucaaaga cgcacaugca aaacgccaaa ccguagucgu acucggcagc caggaagggg     1440

ccgugcacac agcccuagca ggagcccucg aagccgaaau ggacggagcc aaaggacgac     1500

ucucaagcgg ccaccucaaa ugccgacuaa aaauggacaa acuacgacua aaaggcguca     1560

gcuauagccu gugcaccgca gccuuuaccu uuacuaagau ucccgcggaa acccuacaug     1620

gcaccgucac cguagaggua caauacgccg gcaccgacgg cccaugcaaa guccccgccc     1680

aaauggccgu cgacaugcaa acgcucacac cagucggaag acuaauaaca gccaaccccg     1740

ucaucaccga aaguaccgaa aacaguaaaa ugaugcuaga acucgaucca cccuucggag     1800

acucauacau cgucaucgga gucggcgaaa agaaaauaac acaccauugg cauaggucgg     1860

gcuccacaau aggaaaagca uucgaagcca ccguccgcgg ugcaaaacga auggcagucc     1920

uaggggacac agcaugggac uucggcagcg ucggaggugc acuaaacucc cucggcaaag     1980

gcauccacca aaucuucggc gcagccuuca aaucccuguu cggaggcaug uccugguuca     2040

gccaaauccu caucggcacc cuauuaguau ggcuaggccu caacacgaaa aauggcucga     2100

uaucacuaac augucucgcc cuaggcggag uccucaucuu ccuaagcacc gccguauccg     2160

cagacgucgg augcuccgua gacuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7314
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 2 opt

<400>  7314
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uuauaaacgc ccgcaaagag aagaaaagac gcggagccga cacauccgug gguaucgucg      120

gacuccuccu aaccaccgcc auggccgucg aaguaacccg acgcggaucc gcguacuaca      180

uguaccuuga ccgcucagac gcgggggaag ccauaucuuu ucccaccaca cuaggaguca      240

acaaaugcua cauccaaauc auggaccuag gccacaugug cgacgccaca augagcuacg      300

agugccccau gcucgacgaa gggguugaac cagacgacgu ugacugcugg ugcaacacca      360

cguccaccug gguugucuau ggaaccugcc accacaagaa aggcgaagcc cgccgguccc      420

gccgagccgu cacccugccc agccacagca cacgcaaacu acaaacccga ucucaaacau      480

ggcuagaguc acgagaauau acaaaacacc ucauccgagu cgaaaauugg aucuuccgca      540

acccaggcuu cgcgcucgcc gcagccgcca ucgcauggcu ccucggaucc ucaaccucac      600

aaaaaguuau auaccuggua augauauuac ucauagcacc agccuacuca auacgaugca      660

uaggagucuc caaccgcgac uucgucgagg gcaugagcgg aggcaccugg guagacgucg      720

uccuagaaca cggaggcugu guuaccguca uggcgcaaga caaacccaca gucgacaucg      780

aacucguaac aaccaccguc uccaauaugg cggagguacg aucauauugu uacgaagcau      840

caauaucaga cauggcgagc gacagucgcu gcccgaccca aggcgaagcc uaucuugaca      900

aacaaagcga cacacaguac guaugcaaac gaacacucgu agaccgaggc uggggcaacg      960

gcuguggccu guuuggcaaa gguagccugg uaacaugcgc aaaauucgcc ugcuccaaga     1020

aaaugaccgg caaaucaaua caacccgaaa accuagaaua ccgcaucaug cuguccgucc     1080

acggcagcca acacagcggc augaucguca acgacaccgg acacgaaacc gacgaaaacc     1140

gcgccaaagu agagauaaca ccuaacucac cccgagccga agccacacuc ggaggcuuug     1200

gcagccuagg acucgacugc gaaccccgaa caggacucga cuucuccgac cuauauuacc     1260

uaacaaugaa caacaaacac uggcuaguac acaaagaaug guuccacgac auaccacuac     1320

ccuggcacgc cggagccgac accggaacac cccacuggaa caacaaagaa gcacucgucg     1380

aguucaagga cgcacacgcc aaacggcaga ccgucguugu ccuaggcuca caagaaggcg     1440

cgguccacac cgcucucgca ggcgcccucg aagcagaaau ggacggcgcc aaggggaggc     1500

ucuccuccgg acaccucaag ugccgccuaa aaauggacaa acuacggcuc aaaggaguaa     1560

gcuacagccu cugcacagca gcauucacau ucaccaaaau accagcagaa acgcuacaug     1620

gaacagucac ggucgaagua caauacgcgg gcacagacgg acccugcaaa gugcccgcac     1680

aaauggcggu ugacaugcaa acccuuacgc cagucggccg acucaucacc gccaaccccg     1740

ucauuacaga auccacagaa aacuccaaaa ugaugcuuga acuggaccca cccuucggcg     1800

acucguacau aguaauaggc gucggcgaca agaaaaucac ccaccacugg uaccgcuccg     1860

gcucaacaau cggaaaagca uucgaagcaa ccguccgagg ugccaaacgu auggcaguac     1920

uaggcgacac cgccugggac uucggcuccg uaggaggagc acucaacucc cucggaaaag     1980

gaauccauca aauauucgga gccgccuuca agucccuauu cggcggcaug ucaugguuua     2040

gccagauccu cauaggaacc cugcuaguau ggcuaggccu caacaccaaa aacgggucaa     2100

ucucuuuaac cugccuagca uuagggggag uucucauauu ccucagcaca gcaguaagcg     2160

ccgacguagg cuguuccguc gauuucucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7315
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 2 opt

<400>  7315
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaaucaacgc acgcaaagaa aagaaaagac gcggagcaga cacaagcgug ggaauuguag      120

gccugcuauu aaccacugcc auggcgguag aaguaacacg acgcggauca gcauacuaca      180

uguaccugga cagaagcgac gcuggcaaag ccaucuccuu cccaacaacg cugggaguaa      240

acaaaugcua cauccaaaua auggaccuag ggcacaugug cgacgccaca augagcuacg      300

aaugcccgau gcucgacgag ggaguagaac cagacgacgu cgacugcugg ugcaacacaa      360

cuagcacaug gguaguauac ggcaccuguc accacaagaa aggagaagca cgccggagcc      420

gacgggcagu cacgcuaccc ucacacagca cacgaaaacu acaaacucgc ucccaaaccu      480

ggcucgaaag ccgagaauac accaaacacc uaauccgcgu cgagaacugg aucuuccgca      540

acccaggauu cgcacuagca gcugcagcaa ucgcguggcu gcucggaagc agcacaagcc      600

aaaaaguaau auaccuagua augauacucc ucaucgcccc cgccuauagu auacggugca      660

ucggcgucag caaccgagac uucgucgaag gcaugagcgg cggcacgugg gucgacgucg      720

uccucgaaca uggcggaugc gucacaguaa uggcccagga caaaccaacc gucgacaucg      780

aacuugucac aacgaccgug agcaacaugg ccgaaguccg cuccuacugc uaugaagccu      840

ccaucucaga uauggccuca gacagccgau gcccaaccca gggcgaggca uacuuggaca      900

aacaaagcga cacccaauac gucugcaagc gaacacuggu cgaccgaggg ugggguaaug      960

gaugcggccu auucggcaag ggaagccuag ucaccugugc aaaguucgcc ugcucaaaga     1020

aaaugacagg gaaauccauc caacccgaaa accucgagua ccgcauaaug uugagcgucc     1080

acggcuccca acacagcggc augauaguaa acgacaucgg ccaugaaacc gacgaaaauc     1140

gagcaaaagu agaaauaaca ccuaacaguc cgagagcgga agcaacacua ggaggcuucg     1200

gcucgcuagg ccuagacugc gaaccccgaa ccggacucga cuucagcgac cuauacuauc     1260

ucacuaugaa caacaaacac uggcucgugc acaaagagug guuccacgac aucccgcucc     1320

ccuggcacgc aggagccgac acaggcaccc cacacuggaa caacaaagaa gcgcuagucg     1380

aguucaaaga cgcacacgcu aaaagacaaa ccguggucgu ccuaggcuca caagaaggcg     1440

caguacacac agcacuagcc ggcgcucucg aagcagaaau ggacggagcc aaaggucgac     1500

uauccuccgg ccaccuaaaa ugccgccuca aaauggauaa acuccgccuc aaaggcguau     1560

cauacagucu augcaccgcc gcauucacau ucacaaaaau accagccgaa acccuccacg     1620

gcaccguaac cguagaagua caguacgcag gaaccgacgg accaugcaaa gugccagccc     1680

aaauggcagu cgacaugcaa acacucacuc cagucggacg acuaaucacc gcuaaccccg     1740

ucauaaccga gagcacggaa aauuccaaga ugaugcuaga acuggauccc ccguucggcg     1800

acagcuacau agucaucgga gucggagaca agaaaaucac acaccacugg caccgaagcg     1860

gcagcaccau cggaaaagcg uucgaagcaa ccgucagagg cgccaaacga auggccguac     1920

ucggagacac ggccugggac uucggcuccg ucggcggagc acucaauucc cucggaaaag     1980

guauccacca aauuuucggc gccgcauuca aaucacuauu ugggggcaug uccugguucu     2040

cgcagauacu uaucggcacc cuccuaguau ggcuaggacu aaacaccaaa aacggcagca     2100

uaagccucac augccuagcc cucggagggg uccuaauauu ccugagcaca gccguaagcg     2160

cugacgucgg cugcagcguc gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7316
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 2 opt

<400>  7316
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

ucaucaacgc aagaaaagaa agaaaaagaa gaggcgccga cacaucaauc ggcaucgucg      120

gccugcuacu aacaacagcu auggcagcag aaaucacccg acgaggcuca gcguacuaca      180

uguaccucga cagaagcgac gccggaaaag cuaucagcuu cgccacgaca cugggaguca      240

acaagugcca cgugcagaua auggaucucg gacacaugug cgacgcaacc augaguuaug      300

agugccccau gcuagacgaa ggcguagagc cagacgacgu agacugcugg ugcaacacaa      360

caagcaccug gguaguauac ggcacaugcc accacaagaa aggagaagca agacgaagca      420

ggcgcgcagu aacacuaccc ucacacagca cacgaaaacu ccaaaccaga agccagacau      480

ggcucgagag ccgugaauac acaaaacacc uaauaaaagu ggaaaacugg auauuccgca      540

acccaggguu cacccuagua gccguagcaa ucgcguggcu ucucgggagc agcaccuccc      600

agaaagucau auaccucgua augauccuuc ucauagcucc ggccuacagc auacgaugca      660

uaggcguaag caaccgcgac uucgucgaag gaaugucagg gggaacaugg guggacguag      720

uacuagaaca cggaggcugc gucacgguaa uggcacaaga caaaccaacc gucgauaucg      780

agcuagucac caccacaguc agcaacaugg cggaaguacg aagcuacugc uacgaagccu      840

caaucagcga cauggcgagc gacucucgcu gcccaacaca aggcgaagca uaccucgaua      900

aacaaagcga cacgcaguac gucugcaaac gcacccucgu cgacagaggg uggggcaacg      960

gguguggccu auuuggaaag ggaucauuag ucacaugcgc aaaauucaca ugcagcaaga     1020

aaaugacagg gaaaagcauc caaccugaga accucgaaua ccgcauaaug cucagcgucc     1080

auggaucaca acacuccggc augaucguca acgacacggg acacgaaaca gaugaaaauc     1140

gagcgaaagu agaggucaca ccgaauagcc ccagagccga agccacacua ggaggauucg     1200

gaagccucgg ccuggacugc gaaccgcgaa ccggccucga cuucagcgac cuauacuacc     1260

ucaccaugaa caacaaacau uggcuagucc acaaagaaug guuccacgac aucccccucc     1320

ccuggcacgc aggcgccgac acgggaaccc cucacuggaa caacaaagaa gcacugguug     1380

aguucaaaga cgcacacgcc aaaagacaaa caguaguagu auuagggagc caggaaggag     1440

ccguacacac agcccucgca ggcgcccuag aagccgaaau ggacggagcc aaaggcagac     1500

uguucagugg gcaccucaaa ugcaggcuaa aaauggacaa acuacgccua aaaggcguaa     1560

gcuacagccu augcacagcc gcauucaccu ucacaaaggu ccccgcggaa acacugcaug     1620

ggacgguaac aguagaagug caauacgcag gcacagaugg acccugcaaa guaccagcac     1680

aaauggccgu agacaugcaa acacuaacac ccgucggaag acucaucacc gcaaacccag     1740

ucaucacgga aucaacagaa aacagcaaaa ugauguuaga acuggaccca cccuucggcg     1800

acucauacau aguaauaggc gucggagaca agaaaaucac gcaucacugg caccgcagcg     1860

gcagcacaau uggcaaggca uucgaagcaa cagucagagg agcgaaacga auggcaguac     1920

ucggcgauac agccugggac uuuggauccg ugggaggcgu guucaacagc cucggaaaag     1980

guauccacca aaucuucggc gcagcguuca aaucacuauu cggcggcaug agcugguuca     2040

gccaaauacu caucggaaca cuccuaguau ggcuaggacu caacaccaaa aacggcagca     2100

ucagccugac augccuagcg cucggaggag ucaugauauu ccucucaacc gcagugucgg     2160

ccgacguagg gugcaguguc gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7317
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 2 opt

<400>  7317
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaacgc ccgcaaagag cgcaaaagaa ggggagcaga cacgagcaua ggcaucaucg      120

gacuccugcu caccaccgca auggcagcag aaaucaccag aagaggauca gccuacuaca      180

uguaccugga cagaagcgac gcgggaaaag caauauccuu cgcaacgaau cuaggcguca      240

acaaaugcca cguacagauc auggaccuag gucauaugug cgacgccacc augucauacg      300

aaugcccaau gcuagacgaa ggcgucgaac cagacgacgu cgacugcugg ugcaacacca      360

cguccaccug ggugguauac ggcacaugcc accacaagaa aggcgaagcc cgaaggagca      420

gacgcgcugu cacccuacca agccacagua cgcgcaagcu ccaaacaaga agccagaccu      480

ggcuagaaag ccgcgaauac acaaagcacc uaaucaaagu cgaaaacugg aucuuccgaa      540

acccaggauu cgcccucgca gccgucgcaa ucgcauggcu gcuaggaagc uccaccagcc      600

aaaagguaau cuaccugguc augauccuac ugaucgcacc cgccuacucg auucgaugca      660

ucggcguauc gaaccgcgac uucgucgaag guaugagcgg cggaaccugg gucgacgucg      720

uacuagaaca uggcggcugc guuacaguaa uggcacaaga uaagcccacc guagacaucg      780

aacucgucac cacgaccgua agcaacaugg cagaaguccg cucuuacugc uacgaagcaa      840

gcaucagcga cauggcaagc gacucacgau guccaaccca aggggaagca uaccucgaca      900

agcaaagcga uacacaauac guaugcaaaa ggacccucgu cgaccgaggc uggggaaacg      960

gaugcggacu cuucggcaaa ggaagccuag uaacaugcgc caaauuuaca ugcagcaaga     1020

aaaugacagg caaaaguauc cagccagaaa accucgaaua ccgcaucaug cuuagcguac     1080

acggcagcca acacucagga augaucguca acgacauagg acacgaaacc gacgaaaaca     1140

gggcgaaagu agaagucaca cccaacuccc cacgagcaga agcuacccug ggcggauucg     1200

ggagccuggg ccucgacugu gaaccgagaa caggacuaga cuucagcgac cucuacuacc     1260

ucacaaugaa caacaaacac uggcuggugc acaaagaaug guuccaugac auaccgcucc     1320

cauggcacgc aggagccgac acaggaacac cgcacuggaa caacaaagaa gcccuagucg     1380

aguucaaaga cgcacacgcg aaaaggcaga cagucgucgu ccugggauca caggaaggcg     1440

caguacacac agcacucgcc ggcgcguuag aagcagagau ggacggugcc aaaggacgac     1500

uguuuucagg gcaccucaaa ugccggcuaa aaauggacaa acugcgccuc aaaggaguau     1560

cauacagcuu augcaccgcc gccuuuaccu ucaccaaagu ccccgcagaa acccuccacg     1620

gcaccguaac aguggaagua caauacgcag ggaccgacgg gccaugcaag guaccugcac     1680

aaauggccgu cgacaugcaa acacucacac ccgucggaag acuaaucacc gccaacccag     1740

uaauaacaga aagcacagaa aacagcaaaa ugaugcuaga acuagaccca ccauuuggag     1800

acagcuacau cgugaucgga gugggcgaca agaaaauaac ccaucacugg caccgcucag     1860

gcagcacaau aggcaaagcc uucgaagcaa cagucagagg agcgaaaaga auggccguac     1920

ugggagacac ugccugggac uucggcuccg ucggcggggu auucaacagc cuuggcaaag     1980

gcgugcacca gauauucggg gccgcguuca aaaguuuauu cgggggaaug aguugguucu     2040

cccaaauacu aauaggcaca cuucuaguau ggcucggacu aaauacgaaa aacggaagca     2100

uuagccucac augcuuagcc cucggcggag uuaugauauu ccucaguaca gcagugucag     2160

ccgacguggg augcuccgua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7318
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 2 opt

<400>  7318
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucauaaacgc acgcaaagaa aggaaacgca gaggagcgga caccuccaua ggaauaauag      120

gccuacuacu cacaaccgcc auggcggccg aaaucacgcg acgaggaucu gcguacuaca      180

uguaccugga ccgcagcgau gccggaaaag ccaucuccuu cgcaacgacg cucggaguca      240

acaagugcca uguacaaaua auggaccucg gucacaugug cgacgccacg augucauacg      300

aaugcccgau gcucgacgaa ggcguagaac cagacgacgu cgauugcugg ugcaacacaa      360

cgagcacaug ggucguauac ggcacuugcc accacaaaaa gggagaagca cgccgcagcc      420

gccgagcagu cacacugcca ucccacagca cgcgcaaacu acaaaccagg agccaaacgu      480

ggcuagaaag cagagaauac acgaaacacc ugaucaaagu cgaaaacugg auauuccgaa      540

accccggauu cgcccuggua gcaguagcaa ucgcauggcu acuaggcucc agcacauccc      600

aaaaggucau auaccuagua augauacuac ucaucgcacc agccuacagc auaagaugca      660

ucggcguaag caaccgcgac uucgucgaag gaauguccgg cgggaccugg guugacguag      720

uacucgaaca cggcggaugc gucaccguga uggcacaaga caaacccacg guagacaucg      780

aacucgucac aacgacaguc agcaacaugg cggaagugcg uucguacugc uacgaagcca      840

gcauaucaga cauggcaucc gacucaagau gcccaacaca aggcgaagcc uacuuagaca      900

aacaaucaga cacccaauac gucugcaaac ggacacuugu cgaccgcgga uggggaaacg      960

gaugcggacu auucggaaaa ggcucgcuag ugacaugcgc aaaguucaca ugcucaaaga     1020

aaaugacagg caaauccaua caaccagaaa accuugaaua ucgaauuaug cucagcgucc     1080

acggcuccca acacucagga augauaguca acgacauagg acacgaaaca gacgaaaaca     1140

gagccaaagu ggaggucaca ccaaacuccc ccagagcaga agcaacacua ggaggauucg     1200

gaucccuggg acuagacugc gaacccagga caggccuuga cuucuccgac cucuacuacc     1260

ucaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auaccccugc     1320

ccuggcaugc cggggcagac acagggacac cccacuggaa caacaaagag gcucugguag     1380

aguucaaaga cgcgcacgcg aaaagacaaa cagucgucgu ccucggcucc caggaaggcg     1440

ccguacauac agcacucgcu ggcgcgcuag aagccgaaau ggacggcgca aaaggcagac     1500

ucuucuccgg acaccuaaaa ugccgacuca aaauggacaa acuacgacug aaaggcguau     1560

ccuacagccu auguacagca gcauucaccu ucacuaaagu accagccgaa acccuacacg     1620

gcacaguaac cguagaaguc caguacgcag gaacagacgg ccccugcaaa guaccagcac     1680

agauggcagu cgacaugcaa acgcugaccc cgguagggag gcucauaacc gccaacccag     1740

uuaucacgga aucaaccgaa aacagcaaaa ugaugcucga acuggaccca cccuucggcg     1800

auucauacau aguaauagga guaggcgaca agaaaauaac ccaccacugg cacagauccg     1860

gcagcaccau aggcaaagcc uucgaggcca caguacgcgg cgccaaaaga auggccguac     1920

ucggagacac cgcaugggac uucggauccg ucggaggcgu auucaacagc cucggcaaag     1980

gaauccacca aauauucgga gccgccuuca aaagccuauu cggaggcaug agcugguuca     2040

gccaaauccu aaucgggacg cuccuaguau ggcuaggccu caacaccaaa aacggaagca     2100

ucagccuaac cugucuagcc cugggcggcg uaaugaucuu ccuaagcacc gcggucagcg     2160

ccgacguagg cugcagcguu gacuuuucaa aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7319
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 2 opt

<400>  7319
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uaaucaacgc aaggaaggaa aggaaaagga ggggcgcaga cacgagcaua gggauaaucg      120

gccuacuacu gacaacagcg auggccgccg aaauaaccag gaggggcagu gcauacuaca      180

uguaccugga cagaagcgac gccgggaaag cgaucucguu cgcgacuaac cucggcguca      240

acaagugcca cgugcaaaua auggaccucg gccacaugug cgacgcaacc augucguacg      300

aaugcccgau gcuagacgaa ggaguggaac ccgacgacgu agacugcugg ugcaacacaa      360

cgagcaccug gguaguauac ggaaccugcc accacaagaa aggcgaagcg cgccggagcc      420

ggcgagcggu cacacuccca agccauagca cacgaaaacu ccagacccgc agccagacau      480

ggcuagaaag cagagaauac acaaagcacc ugaucaaagu cgaaaacugg auauucagaa      540

acccaggguu cgcacuagca gcgguagcca ucgccuggcu gcuaggcagc uccacuagcc      600

agaaggucau auaccucgua augaucuuac ucaucgcgcc agcauacuca auaaggugca      660

ucggcgugag caaccgcgac uucgucgaag gcaugucggg aggaaccugg gucgacgucg      720

uccuagaaca cggcggcugc gucacaguua uggcacagga caagcccaca guagacauag      780

aacucgucac aacaaccgug agcaacaugg ccgaaguacg aagcuacugc uaugaagcgu      840

caaucucaga cauggcauca gacagccgau gccccacaca gggggaagca uaccucgaca      900

agcaaagcga cacccaauac guauguaaac gaacccucgu cgacagagga ugggggaacg      960

gauguggccu auucggaaaa ggcagccucg ugaccugcgc aaaauuuacc ugcucaaaga     1020

aaaugacggg aaagagcauc caacccgaaa acuuagaaua cagaaucaug cuauccgucc     1080

auggauccca acacagcggc augauaguca acgacgaaaa cagagccaaa guggagguca     1140

cgccaaacag cccaagagcc gaagccaccc ucggcggauu cggcagcuug ggccuagauu     1200

gcgaacccag gaccggacua gacuucuccg aucuuuauua ccugaccaug aacaacaaac     1260

acuggcuagu acacaaagaa ugguuccacg acaucccacu accauggcac gcaggagcag     1320

acacagggac cccgcacugg aauaauaaag aagccuuggu cgaguucaaa gacgcacacg     1380

cgaaacgcca aaccguagua guacuaggcu cccaagaagg ugcgguccac accgcucuag     1440

ccggagcccu cgaagcagaa auggacggcg ccaaaggccg acuguucuca ggccaccuaa     1500

aaugucgacu aaaaauggac aaauuacgcc ucaaaggcgu aaguuacucg cucugcacag     1560

cggcauucac cuucaccaaa gugccagccg aaacuuuaca cggaaccgua accgucgaag     1620

uacaauaugc gggaacagac ggacccugca aaguuccagc ucaaauggcg gucgacaugc     1680

agacgcucac ccccgucggc cgacucauaa ccgccaaccc cgucauaacu gaaagcacag     1740

aaaacucaaa gaugauguua gaauuagacc cacccuucgg cgacucauac aucguaauag     1800

gcguaggaga caagaaaaua acccaccacu ggcaccgauc agguagcacg auagguaaag     1860

cauucgaagc cacagucaga ggcgcaaaac gcauggcagu gcugggcgac acagcguggg     1920

acuucggauc cgucggcgga guauucaacu cacucgggaa agggauccac caaauauucg     1980

gagccgcauu caaaucccua uucggcggaa ugagcugguu cucccaaaua cuaauaggca     2040

ccuuauuggu auggcuaggg cucaacacca aaaacggaag cauaagccug accugccuag     2100

cacucggagg agucaugaua uuccucagca ccgcagucag cgcagacguc ggaugcagcg     2160

uggacuuuag caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7320
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 2 opt

<400>  7320
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagaa       60

uuaucaacgc ccgaaaagaa cgcaagcggc gaggcgcaga caccucaaua ggaaucgucg      120

gccuacuacu uaccacagcg auggcggcag aaaucacacg acgcggcagu gcauacuaua      180

uguaccuaga ccgcucagac gccggcaaag caaucaguuu cgcaaccacc cucggcguca      240

acaagugcca cguccaaauu auggaccuag gccacaugug cgacgccacc auguccuacg      300

aaugccccau gcuugacgaa ggagucgaac ccgacgacgu cgacuguugg ugcaacacca      360

caagcaccug ggucgucuac ggcacauguc accacaagaa aggcgaagcg cgaaggagca      420

gacgagcggu aacccuacca ucccauucca cccguaaacu ucaaacacgc agccaaaccu      480

ggcucgaaag ccgagaauac acaaaacacc uaaucaaagu cgaaaauugg auauuccgca      540

acccgggguu cgcucuaguc gccguggcca uagccuggcu ccuaggcucc ucaacgucac      600

aaaagguaau auaccucguc augauacucc uaaucgcacc agcguacagc auccgaugca      660

uaggcgucag caaccgcgac uucguggagg ggaugagcgg aggcaccugg guagaugucg      720

uacucgaaca uggaggcugc gucacaguca uggcacaaga caagccaaca guggacauag      780

aacucgucac aacgacgguu agcaacaugg ccgagguacg aagcuacugc uacgaggcca      840

gcauaucaga cauggccagc gacucaaggu gccccaccca aggggaagca uacuuggaca      900

aacaauccga cacccaauac gucugcaaac ggacccuggu ggaccgaggg uggggcaacg      960

gcugcggccu cuucggaaaa gguucccucg uuacaugcgc caaauuuacg ugcagcaaga     1020

aaaugaccgg aaaaucaaua caaccugaaa accucgaaua cagaauaaug cucagcgucc     1080

acggcucgca acacagcgga augaucguaa acgacaccgg acacgaaacc gacgaaaacc     1140

gcgccaaagu cgaagucaca ccaaacuccc cacgcgccga agccacacuc ggcggauucg     1200

guagccuagg acuggacugc gaaccacgca caggacuaga cuucuccgac cucuacuacc     1260

uaaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auaccccucc     1320

cauggcaugc gggcgccgac accggcacac cccacuggaa caacaaagaa gcucuaguag     1380

aguucaaaga cgcacacgcu aaacgccaaa ccgucguagu ccuuggcucc caagaaggcg     1440

cuguacauac cgcccuagcu ggugcccucg aagcagaaau ggacggagcc aaaggacgcc     1500

uguucuccgg ccaccucaaa ugccgccuca aaauggacaa acuccgccuc aaaggaguau     1560

cauacucccu augcaccgca gcuuucacgu ucacaaaagu gccagccgaa acacuacacg     1620

gaacagugac cgucgaaguc caauacgccg gcaccgacgg acccugcaaa guacccgccc     1680

agauggccgu agacaugcag acccuuaccc ccgucggaag acuaaucacc gcaaacccag     1740

ucaucaccga aucaacagaa aauagcaaaa ugaugcucga auuagaccca cccuucggcg     1800

acucauacau cgucauuggc guaggcgaca agaaaauaac ccaccacugg cacaggucag     1860

guuccaccau cggcaaagca uucgaagcca ccguacgagg cgccaaacgc auggcagucc     1920

uaggagacac cgcgugggac uucggcagcg ugggaggagu uuucaacucc cucggaaaag     1980

gaauccacca aaucuuuggc gcggcauuua aaagucuauu uggaggaaug agcugguucu     2040

cacaaauucu gauaggcacc cuccucgucu ggcuaggucu aaacaccaaa aacggcagua     2100

ucucacuaac cugccucgca cuaggagggg uaaugaucuu ccucaguacc gcagucuccg     2160

cagacguugg gugcagcguc gacuucucca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7321
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 2 opt

<400>  7321
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaaucaacgc gaggaaagaa cgaaagagac ggggcgcgga cacauccaua ggcauaguag      120

ggcugcugcu aacaacggcg auggcagccg aaauaacaag aaggggaagc gccuacuaca      180

uguaucucga ccgaucggac gccgggaaag caaucagcuu cgcaaccacc cucggaguua      240

acaaaugcca cguacaaauc auggaccuag gccacaugug cgacgccaca augagcuacg      300

aaugcccaau gcuggacgaa ggcguggaac ccgacgacgu cgacugcugg ugcaacacga      360

caagcaccug gguggucuac ggcacaugcc agcacaagaa gggggaagcc cgacgaucca      420

gacgcgcggu cacacugcca agccacucca cccgcaaacu acagacaagg agccaaaccu      480

ggcucgaaag ccgagaauac accaaacauc ucauaaaagu agagaacugg auauuccgaa      540

acccuggguu cgcccucguc gcgguggcaa ucgcauggcu acuaggaucc agcacgagcc      600

aaaaagucau auaccuagua augauacucu uaaucgcgcc agcauacuca auccgaugca      660

uaggcgucuc caacagagau uucgucgaag gaauguccgg cggcaccugg guagacgucg      720

ugcuagaaca uggaggcugc guaacaguaa uggcacaaga caaaccaaca gucgacaucg      780

agcucgugac aacaaccgua aguaacaugg cggaagugcg cucguacugu uacgaagcca      840

gcaucucuga cauggcaagc gauagcagau gcccaacgca aggcgaagcg uaccucgaca      900

agcaauccga cacacaguau guaugcaaga ggacauuggu agacagaggu ugggguaacg      960

gaugcggacu guucggaaaa ggcucgcuag uaaccugcgc caaauucaca ugcaguaaga     1020

aaaugacagg caaaagcaua cagcccgaga accucgaaua caggauaaug cucucagugc     1080

acggaagcca acacagcgga augauaguaa acgacauugg ccacgaaacu gacgagaaca     1140

gagcaaaagu agaagugaca cccaacagcc caagggcgga ggcaacacua ggcggauucg     1200

gcagccuagg ccuagacugu gaaccgcgaa caggccucga cuucagcgac cuauacuacu     1260

uaaccaugaa caacaaacac ugguuggugc auaaagagug guuccacgac auaccacuuc     1320

cauggcacgc cggagcagac acagggaccc cacacuggaa caacaaagaa gcacuggucg     1380

aguucaaaga cgcgcacgcc aaacgacaga caguagucgu ccugggaagu caagaaggcg     1440

cgguacacac cgcacucgca ggcgcccuag aagccgaaau ggacggcgcc aaaggaaggc     1500

uguucagugg ccaccuaaaa ugcaggcuca aaauggacaa acuucgacug aaagggguaa     1560

gcuacucguu gugcaccgcc guaugcacag ccgccaaagu acccgcggaa acacuacacg     1620

gcacggugac aguggaaguc caguaugccg ggaccgacgg acccugcaag gucccagccc     1680

aaauggcagu agauaugcaa acccucacgc ccguagggcg acuaauaacc gcaaacccag     1740

uaauaacaga aucgacagaa aacagcaaaa ugaugcugga acucgacccg cccuucggag     1800

acagcuauau aguaauaggg gucggagaca agaaaauaac acaccacugg caccggagcg     1860

gaagcacaau ugggaaagca uucgaagcga cagugcgggg agcuaaaagg auggcgguac     1920

uaggagacac cgcaugggau uucgggagcg ucggcggggu auucaacagc cuagggaaag     1980

gcauacauca aauauuuggc gcagcuuuca agagccuauu cgggggcaug agcugguucu     2040

cacaaauacu aauaggaacc cuccuagucu ggcucgggcu caacacaaag aauggaucaa     2100

uaagccuaac augccuugcu cucggaggcg ugaugauauu ccuaucaaca gcggugagcg     2160

cggacguggg augcuccgua gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7322
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 2 opt

<400>  7322
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

uaauaaacgc ccgcaaggaa agaaaacgca ggggcgccga cgccucaaua ggaaucgucu      120

cacuccuacu cacaacagug auggccgcag agaucacgcg cagaggaucc gcauacuaca      180

uguaccuaga cagaagcgac gcaggaaagg caauauccuu cgucacaacc cucggcguga      240

acaaaugcca cguccaaaua auggaccuag gccacaugug cgacgccaca augucauacg      300

agugcccaau gcuggacgaa ggcgucgaac cggacgacgu cgacugcugg ugcaacacaa      360

cguccacaug gguagucuac ggaaccugcc accacaagaa aggcgaagca cggaggagcc      420

ggcgcgcagu aacauuacca ucacacagua cccgaaaacu ccaaaccaga ucccaaaccu      480

ggcuagagag ccgcgaauac accaaacacc uaaucaaagu ggaaaacugg auauucagaa      540

aucccggauu cgcacucguc gccguagcca ucgcauggcu acugggaucc agcacaagcc      600

aaaaaguuau cuaccucgua augauacucc ugaucgcacc cgcauacuca auaagaugca      660

uaggcguaag caaccgcgac uuuguagaag gaaugagcgg agggacgugg gucgacgucg      720

uacuagagca cggaggaugc gucaccguga uggcccagga caaacccacc gucgacauug      780

aacucgucac gaccacagua agcaacaugg ccgaaguacg gucauacugc uacgaagccu      840

caauaagcga cauggcauca gacucacgcu gcccaacaca aggggaagcc uaccuagaua      900

agcaauccga cacccaauac guaugcaaac gcacccuagu cgaccgcgga ugggggaacg      960

gaugcggacu auucggcaaa ggaucgcugg ugaccugcgc caaauucaca ugcucaaaga     1020

aaaugaccgg gaaaagcauc caaccagaaa accucgaaua ccguauaaug cuaucagucc     1080

acggcucuca acauucaggc augaucguca acgacgagaa ccgcgcaaaa guagagguga     1140

cccccaacuc cccaagagca gaggcgaccc ucggaggauu cggcucacug ggccuagacu     1200

gcgaaccccg caccggcuua gacuucagcg accucuacua ccuaaccaug aacaacaaac     1260

acuggcuagu acacaaagaa ugguuccacg acauaccacu ucccuggcau ucaggggccg     1320

auaccgaaac cccgcacugg aacaauaaag aagcccucgu agaguucaag gacgcgcacg     1380

ccaagagaca gacagucgua guccuuggaa gccaggaagg cgccguacac acugcccucg     1440

ccggagcauu agaagccgaa auggacggcg cgaaaggccg acuaucaucu ggccaccuga     1500

aaugccggcu caagauggac aagcugcguc ucaagggagu gucauacuca cucugcacag     1560

ccgcauucac auucaccaag guccccgcug aaacccucca cggaaccgug accgucgaag     1620

uccaauacgc cggacgagac ggaccuugca agguucccgc ccagauggcc gucgacaugc     1680

aaacccuaac acccgucggc agacuaauca ccgccaaccc cgucaucaca gaaucaacag     1740

aaaacucaaa gaugaugcua gaacuagacc caccauucgg ggacagcuac auaguaauag     1800

gcgucggaga caagaaaauc acgcaccacu ggcaccgcag ugggucaaua auaggcaaag     1860

cguucgaagc cacgguccgc ggagccaaac gcauggcagu acuaggcgac accgccuggg     1920

acuucggaag cgucggaggc guguucaacu cacucggcaa aggaauacau caaauauucg     1980

gcgcugccuu caaaucucua uucggaggua uguccugguu cucacaaaua cuaaucggaa     2040

cccuccucgu cuggcucgga cuuaacacca aaaacgggag caucucacug accugccucg     2100

cccucggcgg cguaaugauc uuccuaagca cagcaguauc cgcagacguc ggcugcagcg     2160

uagacuucuc caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7323
<211>  2506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 2 opt

<400>  7323
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

ucauaaacgc ccgcaaagaa agaaaaagaa gaggagccga caccaguauc ggaauaguug      120

gacuccuccu aacaaccgcc auggccgccg aaauaacccg ccgcggcagc gcuuacuaca      180

uguaccucga caggagcgau gcaggcaaag ccaucucauu cgccacaacu uugggcguaa      240

acaaaugcca cgugcaaauc auggaccucg gccacaugug cgacgccacu auguccuacg      300

aaugccccau gcucgacgaa ggcguagaac cugacgacgu agacugcugg ugcaauacaa      360

ccagcaccug gguagucuau ggaacaugcc accacaagaa aggugaagca cgccgaucac      420

ggagagccgu aacccuaccc ucucacagca cccgcaaacu acaaacccgc ucccaaacau      480

ggcuagaguc acgcgaguac accaaacacu uaaucaaagu ggaaaacugg aucuuccgua      540

accccggcuu cgcccucguc gcagucgcca uagccuggcu ccucggcuca uccacauccc      600

aaaaagucau auaccuagua augauacucc uuauugcacc agccuacagc aucagaugca      660

ucggcgucuc aaaccgagau uucgucgagg gcaugucugg aggaaccugg gucgacguag      720

uccucgaaca cggaggcugc gucaccguaa uggcccaaga caaaccaaca guugacauag      780

agcuagugac caccacagug agcaauaugg ccgaaguucg auccuacugc uacgaagcca      840

gcaucucaga cauggccagu gacucucgcu gccccaccca aggagaggcc uacuuagaca      900

aacaaagcga cacacaauac gucugcaagc gcacccucgu cgaccgcgga uggggaaacg      960

guugcggauu auucggcaaa ggcucucucg uuaccugcgc gaaauucacc uguuccaaga     1020

aaaugaccgg caaaagcauc caacccgaaa accuagaaua caggauaaug cucagcgucc     1080

acggcucgca acacuccgga augaucguca acgaugaaaa ccgggccaaa gucgaaguca     1140

cgccgaacuc accaagagcu gaagcuacac uaggcggcuu cggaagccuc ggccuggacu     1200

gcgaaccccg cacaggccuc gacuucucag accucuacua ccucaccaug aacaacaaac     1260

acuggcucgu acacaaagaa ugguuccacg acauaccccu cccauggcac gcaggcgcag     1320

acacaggcac accccacugg aacaacaaag aagcacucgu ggaguucaaa gacgcccacg     1380

caaaacggca aacagucguc guacuaggca gccaagaagg cgcuguccac accgcacuug     1440

caggcgcccu cgaagccgaa auggacggag cuaaaggcag acucuucagc ggccaccuaa     1500

aaugccgccu caagauggac aaacugcgac uuaaaggcgu cucguacucc cuaugcacag     1560

cagccuucac cuucacaaag gucccggcgg aaacacuaca cgggaccgua accgucgaag     1620

uacaauacgc aggaaccgac ggccccugca aaguacccgc acaaauggcg gucgacaugc     1680

aaacacugac ccccgucgga cgccugauaa ccgccaaccc cgucauaacc gagucaacag     1740

aaaacagcaa aaugaugcuc gaacuagacc cucccuucgg cgauucuuac aucgucauag     1800

gagucggcga caagaaaauc acacaccacu ggcacagauc cgguucaaca aucggcaaag     1860

cauucgaggc caccguccgc ggcgccaaac gcauggcagu cuuaggcgac accgccuggg     1920

acuucggcag cguaggcgga guauucaaca gucucggcaa aggcauccac caaauauuug     1980

gcgccgcguu caaaagccug uucgggggca uguccugguu cucccaaauc cucauaggaa     2040

cccuccuagu auggcuugga cuaaacacca aaaacggcuc cauaucccua accugccucg     2100

cucucggcgg ggugaugaua uuccucagca cagcaguguc cgccgacguc ggcugcuccg     2160

uugacuucuc caagugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2220

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2280

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2340

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2460

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2506


<210>  7324
<211>  2524
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 2 opt

<400>  7324
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgaa       60

ucauaaacgc aagaaaagaa agaaaaagga gaggcgccga caccucaauc gguauaaucg      120

gccuacuccu cacaacggcc auggccgcag aaaucacaag acgaggcucc gcauacuaca      180

uguaccucga ccguucagac gcgggcaaag caauaucauu cgccacaacc cucggaguaa      240

acaaaugcca cguccaaaua auggaccuag gacacaugug cgacgccacu augagcuacg      300

aaugcccaau gcuagacgaa ggagucgaac ccgacgacgu cgacugcugg ugcaacacca      360

ccucgaccug gguaguguac gggaccugcc accacaaaaa gggggaaacc cgaagauccc      420

ggaggagugu gagccuaagg uaccacuaca cacgaaaacu ccagaccaga agccaaaccu      480

ggcuggaauc cagagaauac aagaaacacc ucauaauggu agaaaacugg auauucagaa      540

acccaggauu cgcgauaguc agcguggcaa ucacauggcu aaugggaagc cucaccuccc      600

aaaaaguaau auacuuagug augaucgucc ucauaguacc agccuacagc aucagcugca      660

ucggagucag caaccgcgac uuagucgagg ggaugucggg cgggacaugg gucgacguag      720

uacucgaaca uggaggcugc guaacagaaa uggcacaaga caaacccacc guagacaucg      780

agcucguaac caugacaguc ucaaacaugg ccgagguccg gucguacugc uacgaggcgu      840

cccugucaga uauggcaagc gccuccagau gcccuaccca aggcgaacca agucucgaca      900

aacaaucaga cacccaaagc guaugcaaaa gaacacucgg agacaggggu uggggaaaug      960

gcuguggaau auucggaaag ggaagccuag uaacaugcag uaaguuuacc ugcugcaaga     1020

agaugccagg aaaaucaaua caaccagaaa accuggaaua ccgaauaaug cuccccguac     1080

acgguucaca gcacagcggc augaucguaa acgacauugg acacgaaaca gaugaaaaca     1140

gggccaaagu ggaagugaca ccgaacagcc cacgcgcaga agccacccuc ggaggcuucg     1200

gcucacucgg acuggacugc gaaccacgua cggggcuuga cuucagcgac cucuacuacc     1260

ugacgaugaa caacaaacac uggcuaguac acaaagagug guuccaugac auaccccuac     1320

ccuggcacgc cggcgcagau acaggcacgc cucacuggaa caacaaggag gcacucgucg     1380

aguucaaaga cgcccacgca aaaaggcaaa ccguagucgu gcucggcucc caggaaggag     1440

caguacauac agcacuggcc ggagcccuag aagcagaaau ggacggagcc aaaggacgcc     1500

ucuucucagg ccaccugaaa ugcaggcuca aaauggacaa acugagacuc aagggcguaa     1560

gcuacagccu cugcacagca gccuucacau ucaccaaagu gcccgcagaa acacuccacg     1620

gaacggucac cgucgaagua caauccgccg gaacagacgg ccccugcaaa guacccgcac     1680

aaauggcugu cgacaugcaa acauuaacac ccgucggacg acugaucacc gcaaauccgg     1740

uaaucaccga auccacagaa aauagcaaaa ugaugcucga acucgaccca cccuucggag     1800

acagcuacau agucaucggc gucggugaca aaaagaucac ccaccacugg cacaggagcg     1860

gcagcacaau aggaaaagcc uucgaagcaa ccgugcgagg agcgaaaaga auggcagugu     1920

uaggcgacac cgccugggac uucggcucag uaggaggagu guucaacagc cuaggaaaag     1980

gaauacacca aaucuucggc gcggcauuca aaagucuuuu cggaggaaug agcugguuca     2040

gccagauauu aauaggcacc cuccucguau ggcuaggucu aaacaccaaa aacggcucca     2100

ucucacuaac cugccucgcg cuaggcggag ucaugaucuu ccucagcaca gccgucuccg     2160

cagacguagg gugcucaguc gacuucagca aaugaggacu agugcaucac auuuaaaagc     2220

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     2280

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     2340

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2460

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2520

aauu                                                                  2524


<210>  7325
<211>  2518
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 2 opt

<400>  7325
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgca       60

uaauaaacgc ccgaaaagaa cgcaaacgaa gaggcgccga cacaucaaua ggaauaauag      120

gccuccucuu aacgacagcg auggcagccg aaauaaccag gagggguuca gccuauuaca      180

uguaccugga ccgcagcgac gcaggaaaag ccaucucguu cgcaacaacc augggaguca      240

acaagugcca cguacaaauc auggaccuag gacacaugug cgacgccaca auguccuacg      300

aaugcccuau gcuagacgaa ggcguagaac ccgacgacgu cgacugcugg ugcaacacca      360

ccagcacaug ggugguauac ggaaccugcc accacaagaa aggagaagcg agaccgucca      420

gacgagcggu aacacugccg agccacucca cccgcaaacu ucaaaccagg ucacaaacau      480

ggcucgaaag cagagaauau acaaagcacc uaaucaaagu cgaaaacugg auccuccgca      540

acccaggauu cacaguggca gcacuagcaa uggccuggcu acugggaaac agcaccucac      600

aaagagucau cuaccuaguc augauccuac uaaucgcccc cgccuacucg aucaggugca      660

ucggcguuag caaccgggac uucguagaag gcaugagcgg cggaacaugg gucgacguag      720

ugcucgaaca cggcgggugc gucacaguaa uggcacaaga caaacccaca gucgauaucg      780

agcuagucac cacaacagua agcaacaugg ccgaagucag gucguacugc uacgaagcaa      840

gcauaagcga cauggcaagu gacagcagau gccccaccca aggggaagcc uaccuagaca      900

aacaauccga cacccaauac gucugcaagc gcacccuagu cgacagaggg uggggaaacg      960

gcugcggccu auucggcaaa ggaucccuag uaacaugcgc aaaauuuacc ugcagcaaaa     1020

agaugacagg caaaucaauc caacccgaaa accucgaaua cagaaucaug cucucagucc     1080

acgguucaca acacucgggg augaucguca acgauaucgg acacgaaacc gaugaaaaca     1140

gagcaaaggu cgaagucacu ccaaacuccc cuagagccga agcaacccuc ggaggauucg     1200

gcucccucgg ccuagacugc gaaccacgca ccgggcucga cuucagcgac cuauacuacc     1260

uaacaaugaa caacaaacac uggcugguac acaaagaaug guuccacgac auaccacuac     1320

cuuggcacgc aggggccgac accggcacac cccacuggaa caacaaagaa gcgcuaguag     1380

aguucaagga cgcacacgca aaaagacaaa ccgucguagu acuaggaagc caggaaggcg     1440

cgguccacac cgcgcuggcc ggcgcacuag aagccgaaau ggauggagca aaagggcgcc     1500

ucuucuccgg ccaccucaaa ugccggauca aaauggacaa guuaagacua aaaggcgucu     1560

cauacagccu uugcacagca gccuucacau ucucgaaagu gccggcagag acacugcacg     1620

gcaccguaac cgucgaagua caauaugcag gcagagacgg cccaugcaag guacccgcac     1680

aaauggcagu cgacaugcag acacuaaccc cagucggccg ccuaauaaca gcaaacccca     1740

ucaucacaga aagcacagaa aacucccgca ugaugcucga acuagaccca cccuucggcg     1800

acagcuacau aguaaucggg gucggcgaca agaaaauaac acaccacugg caccgaagug     1860

ggagcacaau cggaaaagca uucgaagcca cagucagagg agccaaacgg auggcagucc     1920

ucggcgacac cgcaugggac uucggaagcg ucggaggcgu cuucaacuca uugggcaaag     1980

gaguccacca aguuuuuggg gccgcauuca aaagccuguu cggagggaug agcugguucu     2040

cgcaaauacu aaucggcacc cugcucgucu ggcucggccu caacacaaag aacggaucua     2100

ucucacuugc augccucgca cucggcggcg ccaugaucuu ccuauccacg gcagucagcg     2160

ccgacguagg cuguagcgua gacuucugag gacuagugca ucacauuuaa aagcaucuca     2220

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2280

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2340

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2400

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2460

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2518


<210>  7326
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 2 opt

<400>  7326
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau aguaggccug cuacucacca ccgcaauggc cgcggaaguc accagaagag      120

ggagcgcaua cuacauguac cuagacagga acgacgcggg agaggccaua ucauucccaa      180

cuacgcuagg uaugaacaaa ugcuauaucc aaauaaugga ccuagggcau augugcgacg      240

ccacgaugag uuacgagugc cccaugcuag acgaaggggu cgaaccggac gacguagacu      300

gcuggugcaa cacaaccuca accuggguag ucuacgggac cugccaccac aagaaaggcg      360

aagcacgccg aucaagacga gcgguaaccc ucccaagcca cucgacaaga aaacuccaaa      420

cccgaaguca aaccuggcuc gaaucucgcg aauacaccaa acaccucauu cgaguagaaa      480

acuggaucuu ccggaaccca ggcuucgcgu uagccgccgc cgccauagca uggcuacuug      540

gaagcuccac cucccaaaaa gucauauacc uaguaaugau ccuccucaua gcgccagccu      600

acuccauuag augcauaggc guaagcaaua gagacuucgu cgaaggcaug agcgggggca      660

cguggguuga caucguccug gagcacggcg gcugcguaac aguaauggcc caagacaaac      720

caaccguuga caucgagcua gugacgacaa ccguaucaaa cauggcagaa guaagguccu      780

acugcuacga agcaagcauc uccgacaugg caucagacag cagaugcccg acacaaggag      840

aagcauaccu agacaagcaa agcgacaccc aguacguaug caaaagaacg cuggucgacc      900

gcggaugggg aaacggaugc ggccuauuug gcaaaggaag ccuaguaacc ugcgccaaau      960

ucgcaugcuc caaaaagaug acaggcaaau ccauccaacc agaaaaccua gaauaucgaa     1020

ucauguuaag cguacacgga agucaacacu caggcaugau cgucaacgac acaggacacg     1080

agacagacga aaacagagca aaagucgaaa ucacaccaaa uucaccccga gcagaagcca     1140

cccuaggggg cuucggaucg cucggccuag acugugaacc acgcaccgga cuggauuuca     1200

gcgaccuaua cuaccuaacc augaauaaca agcacuggcu cguacacaaa gaaugguucc     1260

acgacauacc acucccaugg cacgccggcg ccgacacagg gacgccacau uggaacaaca     1320

aagaagcacu cguagaauuu aaagacgccc acgccaaacg acaaacaguc guggucuugg     1380

ggucccaaga aggcgcgguc cacacagcac ucgccggcgc ccuggaagcg gaaauggacg     1440

gcgccaaggg cagacuguca ucgggccacc uaaaaugccg acuaaagaug gacaaacucc     1500

gccucaaagg cguaucauac ucccucugca cggcagcauu cacauucacc aaaauacccg     1560

cggaaacacu acacggcacc gucacaguag aaguccaaua cgccggaacg gacgggccgu     1620

guaagguccc cgcccaaaug gccgucgaua ugcaaacccu gacuccaguc ggccgacuaa     1680

ucacagcaaa ccccgucaua acugaaucca cagagaacag uaagaugaug cucgagcucg     1740

acccacccuu cggcgacagc uacauaguaa ucggagucgg cgagaagaaa auaacacacc     1800

acuggcaccg cagcggaucc accauaggca aagccuucga agccaccguc cgaggugcca     1860

aacgcauggc aguacugggc gacacagccu gggacuucgg cagcgucgga ggagcccuaa     1920

acagccuggg aaaaggcaua caccaaaucu ucggagccgc cuucaaaucc cuuuucggag     1980

gcauguccug guucagccaa auccugaucg gcacccugcu uauguggcua ggccuaaaca     2040

ccaaaaacgg auccaucagc cuaaugugcc uggcccuagg cggugugcua aucuuccuaa     2100

gcacugccgu aucagcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7327
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 2 opt

<400>  7327
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacuu       60

cagucggcau cguaggccua cuccucacca ccgccauggc agccgaagua acccgccgcg      120

gcuccgcaua cuacauguac cucgacagga acgacgcugg cgaagccauc uccuucccca      180

caacccucgg aaugaauaaa ugcuacaucc aaaucaugga ccucgggcac acaugcgacg      240

caacuauguc cuaugaaugc cccaugcuag acgaaggcgu cgaacccgau gacgucgauu      300

gcuggugcaa cacaaccuca accuggguag ucuacggaac cugccaccau aagaaaggcg      360

aagcccgcag aucccgacgc gccguaacac uaccguccca cuccacgcgc aaacuccaga      420

caaggagcca gacauggcua gaaagccgcg aauacaccaa acaccuaaua agaguagaga      480

acuggaucuu ccgaaaccca ggauucgcac ucgcagcagc agccaucgca uggcuacucg      540

gauccagcac cucccaaaaa guaauuuacc uggucaugau ccugcuaaua gcgcccgcau      600

acagcaucag gugcauaggg gucuccaaua gagacuucgu agaaggcaug agcggcggaa      660

cguggguaga cgucguacuc gaacacggcg gcugcgucac uguaauggcc caagacaaac      720

cuacgguuga caucgaacuc guaacaacaa ccgucucaaa cauggccgaa guacgauccu      780

acugcuacga agccucaaua uccgacaugg ccuccgacag ccggugcccc acgcaaggcg      840

aagcauacuu agacaaacaa agcgacacac aauacgucug caaacgaaca cuggucgacc      900

gcggcugggg aaacggcugu ggacuauucg gaaaaggcuc acucguaaca ugcgcaaaau      960

ucgccuguag caagaaaaug acaggaaaaa gcauccaacc cgaaaaccuc gaauaccgaa     1020

ucaugcuguc aguccacgga agccaacauu caggaaugau cgucaacgac acaggacacg     1080

aaacugacga aaacagagcc aaggucgaga uaacaccaaa cagcccacgu gcagaagcca     1140

cccuaggagg guucggguca cucggccuug acugcgaacc gcggacggga cuagacuuca     1200

gcgaccuuua cuaccucaca augaacaaca aacacuggcu aguucauaaa gaaugguucc     1260

acgauauccc acugcccugg cacgcaggcg ccgacacagg cacaccacac uggaacaaca     1320

aggaagcccu cgucgaguuc aaagacgccc acgcgaaaag acaaaccguc gucguacuag     1380

gcucacaaga gggcgccguc cacaccgccc uagccggcgc ccuagaggca gaaauggacg     1440

gcgcaaaggg ccgccucuca uccgggcacc ucaaaugccg ccucaaaaug gacaaacucc     1500

gucuuaaagg cgucucguau ucccuaugca ccgccgccuu cacguucacc aaaauaccgg     1560

cagaaacgcu ccacggaacc gucaccguag aaguacagua ugccggcacg gacggccccu     1620

gcaaaguccc agcccaaaug gccgucgaca ugcagacacu caccccaguc ggcaggcuaa     1680

ucacggcgaa ucccgucauc acagaaucca cagaaaacuc aaaaaugaug cuagaacuag     1740

acccgccauu cggcgacagu uacaucguca ucggagucgg cgaaaagaag aucacacauc     1800

acuggcacag aagcggcagc acaauaggga aggccuuuga agcaaccguc agaggugcca     1860

aaagaauggc aguccucggc gacaccgccu gggacuucgg aucgguaggc ggugcccuca     1920

acucccuagg aaagggcauc caccaaaucu ucggugccgc auucaaaucc cuauucgggg     1980

gcaugucaug guucucacag auacuaauag gaacccuccu cauguggcua ggccuaaacg     2040

ccaaaaacgg cagcauaagc cugaugugcc uagcccucgg cggcguacua aucuuccuca     2100

gcacagccgu cucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7328
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 2 opt

<400>  7328
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gcauaggcau aauaggacuc uugcucacaa ccgcaauggc cgccgaaauc acgcgacgag      120

gcucagccua cuacauguac cuagacagaa gcgacgcagg caaagccauc ucguucgcaa      180

ccacacucgg aguaaacaaa ugccacguac aaauaaugga ucucggacac augugcgaug      240

caacaauguc cuacgagugc cccauguuag acgaaggagu agaaccggac gacgucgacu      300

gcuggugcaa uaccacaagc acguggguag uauauggcac gugccaccac aagaaaggcg      360

aagcacgcag aagccggcgc gccgucacgc uacccagcca uagcaccaga aagcuccaga      420

cacgguccca aaccuggcuc gagagccgcg aauauacaaa acaccugauc aaggucgaaa      480

acuggaucuu ccggaauccu ggauucgcac ucgucgccgu ugcaaucgcu uggcuccuag      540

gcucgagcac gagccaaaaa guaaucuacc ucguaaugau acuacuaauc gcgcccgcau      600

auucuauccg augcauaggc gugagcaacc gugacuucgu cgaaggaaug ucgggcggga      660

caugggucga uguaguacua gagcacgggg gaugcgucac ggucauggca caagacaagc      720

ccaccgucga uaucgagcuc gucacgacca cagugagcaa cauggcugaa gugcgcucau      780

acugcuacga agccuccaua ucagacaugg cauccgacag cagaugcccu acacaaggug      840

aagcauaccu ggacaaacaa ucagacacac aauacgucug caaaaggacc cuagucgauc      900

gcggaugggg gaacgggugc ggacuauucg gcaaaggaag ccuagugacc ugcgcuaaau      960

uuaccugcuc gaagaaaaug acaggaaaga gcauccaacc cgaaaaccuc gaauacagaa     1020

ucaugcucuc aguccacggc ucucaacaua gcgggaugau aggcuacgaa acugacgagg     1080

acagagccaa agucgagguc acgcccaaca gcccgcgcgc agaagcuaca cugggaggau     1140

ucggcagccu aggacuagac ugcgaaccac gcacaggacu cgacuucagc gaccuauacu     1200

accugacaau gaacaacaaa cacuggcuag uccacaaaga augguuccac gauauaccgc     1260

ucccguggca cgccggcgcc gacaccggua ccccacacug gaacaacaaa gaagcacucg     1320

ucgaguucaa agacgcacac gcaaaaagac aaacggucgu aguauuaggc ucgcaagaag     1380

gcgcagucca cacagcccua gcaggcgcgc uagaagcaga aauggacggc gcgaaaggca     1440

gacuauucuc aggccaucuc aagugccgcc uaaaaaugga caaacuccgc cuaaaagggg     1500

uauccuacuc acucugcaca gcagcauuca cauucaccaa aguccccgcg gaaacccuac     1560

auggcacagu aaccguagaa gugcaauaug ccggaacgga uggccccugc aagaucccgg     1620

uacaaauggc gguugacaug cagacucuga ccccaguagg gcgccuaauc acagcaaacc     1680

cuguaaucac ugaauccaca gaaaacucaa aaaugaugcu cgaacuggac ccaccauucg     1740

gggacagcua cauaguaaua ggagucggcg acaagaaaau cacccaccac uggcacagaa     1800

gcgggaguac aaucggaaaa gcguucgagg ccacagucag gggcgcaaaa agaauggcag     1860

uacuaggaga caccgcuugg gacuucggca gcgugggcgg gguauucaac ucacucggaa     1920

aaggcaucca ccaaaucuuc ggugcagccu ucaaaagccu guucggagga auguccuggu     1980

ucucccaaau ucucaucgga acgcuccuag ucuggcucgg acuaaauacc aaaaacggau     2040

cuaucagcuu aaccugccuc gcgcuaggag gagugaugau cuuccugucc acagcagugu     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  7329
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 2 opt

<400>  7329
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguaggcau cguaggccua cuccugacga ccgccauggc cgccgaaguc acccgccgcg      120

gcuccgcaua cuacauguac cucgaccgaa acgacgccgg agaagccauc agcuuuccca      180

cuacccucgg caugaacaaa ugcuacaucc aaaucaugga ccucggucac augugcgacg      240

cuacuaugag cuacgaaugu cccaugcuag acgagggagu ugaacccgac gacgucgacu      300

gcuggugcaa cacaacauca acaugggucg uauacggcac cugucaccac aagaaaggcg      360

aagcaagacg uucuagacga gccguaacac uaccauccca uucgacccgc aaauuacaaa      420

cccgcuccca aaccuggcua gaaucacgag aauacaccaa acaccucauc cgcgucgaga      480

acuggaucuu caggaacccg ggauucgcac uugcugccgc agcaauagca uggcuccucg      540

gauccuccac cucccaaaaa guuauauacc ucgucaugau ccuccuuauc gcccccgcau      600

acucgauccg cugcauaggu gucucaaacc gagacuucgu ggaaggcaug uccgggggca      660

cguggguaga cguggucuua gaacacggcg gcugcguaac cguaauggca caagacaaac      720

caacagugga caucgaacug gucaccacaa caguauccaa cauggccgaa guccgcagcu      780

auugcuacga agcaagcaua ucugauaugg cguccgacuc acgcugcccc acucagggcg      840

aagccuaccu ugacaagcaa uccgacaccc aauacgucug caaacgcaca cucgucgacc      900

gaggcugggg caacggaugc ggacuauucg gaaagggcuc ccuagucaca ugcgccaaau      960

ucgccugcuc caagaaaaug accggcaaau cgauacaacc cgaaaaccuc gaauaccgua     1020

uaaugcuauc cguccacggc ucacaacauu cagggaugau cgucaacgac acaggccacg     1080

aaaccgacga aaaccgagcc aaggucgaaa uaaccccgaa cagccccaga gccgaagcca     1140

cccuaggagg cuucggcucc cuaggccuag acugcgaacc ccgcaccggc cucgacuucu     1200

ccgacuugua cuaccucacg augaacaaca aacacuggcu aguccacaaa gaaugguucc     1260

acgacauacc ccucccaugg cacgccggcg cagauaccgg aacuccccac uggaacaaca     1320

aagaagcccu agucgaguuc aaagacgccc acgccaaacg ccaaacaguc guaguccuag     1380

gaucccaaga aggcgccguu cacacggcac ucgccggagc acuagaagcc gaaauggacg     1440

gcgcaaaagg ccgucuaucc ucaggccacc uuaaaugccg ccugaagaug gauaaacucc     1500

ggcucaaagg agucuccuac ucacugugca ccgccgcauu cacauucacc aaaaucccag     1560

ccgaaacccu ccacggcaca guaaccguug aaguccaaua cgccggcacc gauggcccau     1620

gcaaaguacc ggcccaaaug gcaguagaca ugcaaacacu cacacccgua ggacgccuca     1680

uaacggccaa cccaguaaua accgaaucca cagaaaacuc caaaaugaug cuagaacuag     1740

acccacccuu cggggacucc uacaucguca ucggcguagg cgaaaagaaa aucacacauc     1800

acuggcaccg cucaggauca acaauaggaa aagccuucga agccacagua agaggugcaa     1860

aacgcauggc aguacuagga gacacagcau gggacuuugg gucagucggc ggagcccuga     1920

acucacucgg aaaagggauc caccaaaucu ucggcgccgc auucaaaucc cuauucggag     1980

gaaugagcug guucagccaa auccucaucg ggacccuacu aauguggcuc ggccucaaca     2040

ccaaaaacgg cuccaucucc cuuauguguc ucgcucuagg cggcguacuc auauuccuca     2100

gcaccgccgu cucugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7330
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 2 opt

<400>  7330
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gcaucggcau cauaggucuc cuccuaacca cggccauggc cgccgaaauc acccgccgcg      120

gcuccgcaua cuacauguac cuugaccgcu cagaugccgg aaaagcaauc ucauucgcga      180

caacccucgg aguaaacaaa ugccacguac aaaucaugga ccugggccau augugcgacg      240

ccacgauguc cuacgaaugc cccaugcuag acgaaggggu cgaacccgau gacguagauu      300

gcuggugcaa cacaacaagc accugggucg ucuacggcac augccaccac aagaaaggag      360

aagcacgccg aucacgccga gccgucaccu uacccucuca cucaacccga aaacuacaga      420

caagauccca gaccuggcuu gaaagccgcg aauacacaaa acaccucauc aaagucgaaa      480

acuggauauu ccggaacccc ggauucgccc uaguagcagu agcaauagca uggcugcucg      540

gauccucaac aucacaaaaa guaaucuacc ucgucaugau ccugcuuauc gcacccgccu      600

acucaaucag augcauagga gucagcaacc gcgacuucgu cgaagggaug ucaggaggca      660

cgugggucga cguaguccua gaacacggcg gcugcgucac aguaauggcc caagacaaac      720

cuaccguaga caucgagcuc guaaccacca cuguauccaa uauggccgaa guccguucau      780

auugcuacga agccucaauc uccgacaugg caagcgacuc ccgaugcccc acccaaggag      840

aagcguaccu cgacaaacaa uccgacaccc aguacgucug caaacgaacu uugguagacc      900

ggggcugggg caacggaugc ggucucuucg guaaaggauc cuuaguaacc ugcgcaaaau      960

uuaccugcag caagaaaaug acaggaaaau caauacaacc agaaaaccuc gaauaccgca     1020

ucaugcucuc aguccacggg ucccaacacu ccggaaugau agucaaugac acaggcuacg     1080

aaaccgacga aaaccgcgcc aaaguagaag ucacacccaa cagcccccgc gcagaggcca     1140

cccucggcgg cuucggcucc cuaggccuag acugugaacc gcgaacaggc cuggacuucu     1200

ccgaccucua cuaccuuacu augaacaaca aacacuggcu cguccacaaa gaaugguucc     1260

acgacauccc ucuaccaugg cacgccggag cagacaccgg cacaccccau uggaacaaca     1320

aagaagcccu uguugaguuc aaggacgccc acgcgaaacg acaaaccgug guaguacuug     1380

guucacaaga aggcgcaguc cacacagccc uagccggagc ccucgaagca gagauggacg     1440

gagccaaagg caaacucuuc uccggccacc uaaagugcag acuaaaaaug gacaaacuaa     1500

gacuaaaagg gguaucauac agccuaugca ccgccgcuuu caccuucacg aaagucccag     1560

ccgaaacccu acacggcacc guaaccgucg aaguacaaua cgcaggaacc gacggccccu     1620

gcaaaauccc cguccagaug gccguagaua ugcaaacacu aacccccguc ggacgccuaa     1680

uaaccgccaa cccagucauc accgaaagca cugaaaacuc caaaaugaug cuagaacucg     1740

acccgccauu cggcgacagc uacaucguaa ucggagucgg ggauaaaaag aucacccacc     1800

acuggcaccg cucaggcagc acgauaggca aagccuucga agccacaguc cgcggggcca     1860

aacguauggc aguccuagga gacacggccu gggacuucgg cuccgucggc ggagucuuca     1920

acucccuagg caaaggcauc caccaaauau ucggcgcggc auucaaaagc cuauucggcg     1980

gcauguccug guucucacaa auccuaauag gcacccuccu aguauggcua ggacuaaaca     2040

ccaaaaacgg auccauaagc cuaaccugcc uagcacuagg cggcguaaug auuuuccuaa     2100

gcacggccgu cagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7331
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 2 opt

<400>  7331
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

guaucgguau cauaggccua cugcugacaa ccgcgauggc ugcagagauc acacguagag      120

gaagcgcaua cuacauguac cuggaccgca gcgacgccgg aaaagcaaua ucauucgcca      180

caacgcucgg aguaaacaaa ugccacgucc agauaaugga ccucggucac augugcgacg      240

caaccauguc guacgaaugc cccaugcuag acgagggagu cgaaccagac gacgucgauu      300

gcuggugcaa cacaaccagc acgugggucg uguacggaac cugccacaga aagaaagggg      360

aagcgagaag aagcagacga gcggucacac ucccuagcca cagcacacgc aaacuccaaa      420

cccgaagcca aacguggcuc gaaagccgag aauacacaaa acaccucauc aaagucgaaa      480

acuggaucuu ccgcaacccc ggguucgcac ucguggcagu cgccauagca uggcuccugg      540

gcagcagcac gagccaaaaa guaaucuacc uagugaugau acuccugauc gccccagccu      600

acucgauacg augcauaggc gucagcaacc gcgauuucgu ggaaggaaug uccggcggga      660

cguggguaga cguaguccug gaacacggag gcugcgucac cguaauggcc caggacaaac      720

ccacagucga uauagagcug gugacgacaa caguaagcaa cauggcagaa guacgcucgu      780

acugcuauga agcaucgauc ucggacaugg caagcgacag ccgcugucca acccaaggag      840

aggccuaccu agacaaacag agcgacacac aguaugucug caaaagaacc cucguagaca      900

gaggcugggg aaacggaugc ggccucuucg gcaagggauc gcuaguaaca ugcgccaaau      960

ucacaugcuc caagaaaaug acaggcaaaa gcauccagcc cgaaaaccua gaguaccgaa     1020

uaaugcuguc aguccacggc ucacaacacu ccggcaugau aguaaacgac accggcuacg     1080

aaacggauga aaacagagcc aaagucgagg uaaccccgaa cuccccacga gcagaagcaa     1140

cucucggagg guucgggagc cucggguuag acugcgaacc ccgcaccgga uuggacuuca     1200

gcgaccucua uuaccugaca augaacaaca aacacuggcu cguccacaaa gagugguuuc     1260

acgacauccc ccuccccugg cacgcaggcg ccgacacagg aacgccacac uggaacaaca     1320

aagaggccuu ggucgaguuc aaagacgcac acgccaaaag acaaaccgug gucguacucg     1380

gcagccaaga aggagcagua cacacagcgc uagcaggcgc acuagaagcc gaaauggacg     1440

gagcaaaagg gaaauuguuc ucuggccacc ucaaaugcag acugaaaaug gacaaacuaa     1500

gacugaaagg agugucauac ucacugugca cagcagcauu caccuucaca aaaguaccag     1560

cggaaacccu ucacgggaca guaaccguag aaguccaaua cgcaggaacc gacgggccgu     1620

gcaagauccc agugcagaug gccgucgaca ugcagacccu aaccccaguc gggcgacuca     1680

ucaccgccaa cccaguaaua acagaaagua ccgaaaacag caaaaugaug cuugaacuag     1740

acccacccuu cggagacagc uacauaguaa uaggcguggg agauaagaaa auaacccacc     1800

acuggcacag aagcgggagc acaauaggaa aagcauucga ggccaccguc aggggagcaa     1860

aaagaauggc aguacucgga gacaccgccu gggacuucgg gucugucgga ggaguauuca     1920

acucacuagg gaaaggaauc caccaaauau ucggcgccgc cuucaagagc uuauucgggg     1980

gcauguccug guucagccaa auccuaauug gaacccuccu aguguggcua ggccuaaaca     2040

ccaaaaacgg aagcauaagu cuaacauguc uagcccuagg cggcgugaug auauuccuaa     2100

gcaccgcggu aagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7332
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 2 opt

<400>  7332
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcaucggcau aaucggacua uuacucacaa ccgcaauggc agccgaaaua acccgccgag      120

gaucugccua cuacauguac cucgacagga gcgacgccgg aaaagcaaua ucauucgcaa      180

caacccuagg gguaaacaag ugccacguac aaauaaugga ccucggccac augugcgacg      240

ccacaaugag cuacgaaugc cccaugcugg acgagggcgu ggaaccggac gacgucgacu      300

gcuggugcaa caccacuucc accugggucg uauacggaac cugccaccac aagaaagggg      360

aagcacgaag auccagacga gccgucacau uaccaagcca cucaaccaga aaacuacaga      420

caagaagcca aacauggcuc gaaagcaggg aguacacaaa acaccugaua aaagucgaaa      480

acuggauauu ccgcaacccc ggguucgccc ucguggccgu agcaauagcc uggcuacuag      540

gcuccagcac aagccaaaaa gucaucuacc uagucaugau ccuccuaauc gcgccagcau      600

acuccauccg guguauaggc gucucaaaca gagacuucgu cgaagggaug ucaggcggaa      660

ccuggguaga cgugguacuc gagcacggag gcugcguaac agucauggcc caagacaaac      720

ccaccgucga caucgaacug guaacaacca ccguaagcaa cauggcugaa guccgauccu      780

acugcuauga agccagcauc ucagacaugg caagugacuc acgaugccca acccaaggcg      840

aagccuaccu ggacaagcaa agcgacaccc aguacgucug caaacgcacc cuagucgacc      900

gagggugggg aaacggcugc ggccucuucg gaaaaggauc gcuagucacg ugugcaaagu      960

uuaccugcuc caagaaaaug acaggcaaaa guauacagcc ugaaaaccuc gaguaucgaa     1020

ucaugcuauc ugugcacggc ucacaacacu caggaaugac ggucaacgac auaggauacg     1080

agaccgacga aaaccgcgca aaagucgagg ucaccccaaa cagcccacgg gcagaagcca     1140

cccucggagg guucggcagc cuaggccucg auugcgaacc aagaacaggc cuugacuucu     1200

ccgaccuaua cuaucucacc augaacaaca aacacuggcu gguccacaaa gaaugguucc     1260

acgacauacc acuccccugg cacgcaggcg cggacacagg gacgccacac uggaacaaca     1320

aagaagcccu aguagaguuc aaagacgcac acgccaaacg acaaaccguc gucguucuag     1380

gcucccaaga aggcgcaguc cacacggccc uagcaggagc gcuagaagcc gagauggacg     1440

gcgccaaggg caaacucuuc agcggacacc uaaaaugcag gcuaaaaaug gacaaacuac     1500

ggcucaaggg aguaucauac ucgcugugca ccgcggcguu caccuucacc aaaguacccg     1560

ccgagacccu ccacggaacc guaaccgucg aaguccaaua cgcagggacu gacggcccgu     1620

gcaaaauccc aguccaaaug gcagucgaca ugcaaacacu aacaccaguc gggcgacuca     1680

ucacagcaaa ccccguaauc acagaaucca ccgaaaacuc caagaugaug cucgagcuag     1740

acccgccauu cggagacagc uacaucguaa uaggagucgg agacaaaaag aucacccacc     1800

acuggcaccg cucgggaagc acaaucggca aggccuucga agcaacugua agaggcgcaa     1860

aacgcauggc cguacuagga gacaccgcuu gggacuucgg cagcgucggc gggguguuca     1920

acucccuagg aaaaggcaua caccaaauau ucggagcagc uuuuaaaucc cuguucggcg     1980

gcaugagcug guucagccaa auacuaauag gcacccucuu agucuggcua ggacucaaca     2040

caaagaacgg cucaaucuca cuaaccugcc ucgcacuagg aggagucaug auauuccuga     2100

gcacagccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7333
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 2 opt

<400>  7333
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcauaggcau agucggccug cuacucacca cugccauggc cgccgaaaua acacgcagag      120

gcuccgccua cuacauguac cucgaccgcu ccgacgcagg aaaagccauc uccuucgcaa      180

ccacgcucgg cgucaacaaa ugucacguac aaauaaugga ccuaggccac augugcgacg      240

ccaccaugag cuacgaaugc cccaugcucg acgagggcgu cgagccggac gacguagacu      300

gcuggugcaa cacaaccucu acauggguag ucuauggaac cugccaucac aagaaaggcg      360

aggcccgacg aagccgacga gcaguuaccu uacccagcca cagcacacgc aaacuccaaa      420

caagaagcca aacauggcua gagagccgag aauacacaaa acaccucauc aaagucgaaa      480

acuggauauu ccggaaucca ggcuucacac ucgucgcagu agcaauagcc uggcuccucg      540

gcagcagcac cagccaaaaa guaaucuacc uagucaugau ccuccuaaua gcgccagccu      600

acuccauaag gugcauagga guaagcaauc gcgacuucgu agaaggaaug agcggaggca      660

ccugggucga cguaguccuc gaacacgggg gaugcguaac aguaauggcc caagacaagc      720

caacaguaga cauagagcuc gucacaacga ccgucaguaa cauggcagaa gucaggucuu      780

acugcuacga agcaagcauc agcgacaugg caucagacag ccgaugcccc acccaaggag      840

aagcguaccu agacaagcaa ucagacaccc aauacgucug caaaaggacc cuuguagaua      900

gaggaugggg aaacggaugc ggccucuucg gcaagggcag ccuaguaacc ugcgcaaaau      960

ucacaugcuc aaagaaaaug accggcaaau caauccaacc agaaaaccuu gaauaccgaa     1020

ucaugcucuc cguccacgga agccaacaca gcggaaugau agucaacgac gaaaacagag     1080

caaaagucga agucacuccc aacucacccc gagccgaagc cacccugggc ggcuucggau     1140

cccugggacu ugacugcgaa ccucgcaccg gccuagacuu cagcgaccua uacuaccuca     1200

caaugaacaa caaacacugg cuaguccaca aagaaugguu ucacgacaua ccccucccau     1260

ggcacgccgg cgcagacacc ggaacacccc auuggaacaa caaagaagcc cuagucgagu     1320

ucaaagacgc acacgcaaaa cggcagacag uagucguacu aggcucccag gagggcgcag     1380

uacacaccgc acuggccggc gcccuggaag ccgaaaugga uggcgcaaaa ggaaggcugu     1440

ucucgggaca ccucaaaugc aggcugaaaa uggacaaacu aagacucaaa gggguaucau     1500

acuccuuaug cacagccgcc uucaccuuca ccaaaguacc agcagagacg cuccacggca     1560

ccgugaccgu cgagguacaa uacgccggca ccgacggacc augcaaaguc cccgcccaaa     1620

uggcaguaga uaugcagaca cugaccccag ucggcagacu aauaaccgcc aacccgguua     1680

uaaccgaaag caccgaaaac ucaaaaauga uguuggaacu ggacccaccc uucggcgaua     1740

gcuacauagu cauaggugua ggagacaaga aaaucaccca ccacuggcac agaucaggua     1800

gcacaaucgg caaagcguuc gaagccaccg uccgcggcgc aaaaaggaug gccguacucg     1860

gcgacaccgc cugggacuuc ggcagcgugg gaggagucuu caacagccua ggaaaaggca     1920

uccaccaaau cuucggagcc gccuucaaaa gccucuucgg aggaaugagc ugguucuccc     1980

aaauccucau cggaacccua cuggucuggc ucggccucaa caccaaaaac ggcagcaucu     2040

cucuaaccug ccucgcacua ggcggcguca ugauauuccu cagcaccgcg gucucagcau     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  7334
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 2 opt

<400>  7334
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

caaucggaau caucggacua uugcucacca ccgcgauggc agcagaaauc acacgcagag      120

gauccgcaua uuacauguac cuugaccgca gugacgccgg caaagcaauc ucuuucgcca      180

caacacucgg agucaacaaa ugccacgucc aaaucaugga ccucggccac augugcgacg      240

ccacaauguc cuacgaaugc cccaugcucg acgaaggcgu cgaacccgac gacgucgacu      300

gcuggugcaa caccacaucc acuugggucg ucuacggaac cugccaccac aagaaaggcg      360

aggcgagacg cuccagacgc gccgucacac uacccucgca cuccacaagg aaacuccaga      420

cccgaagcca gaccuggcua gagucccggg aauacaccaa acacuuaauc aaaguagaga      480

acuggauauu uagaaacccc ggauucgcuc ucgucgccgu cgcaauugcu uggcuccuag      540

gcucaagcac cagucaaaaa guuauuuacc uagucaugau acuacuaauc gcacccgccu      600

acuccauccg cugcaucgga gucaguaacc gcgacuucgu ggaaggcaug uccggaggaa      660

cgugggucga cguaguccuc gagcacggag gaugcgucac agucauggca caggacaaac      720

caaccgucga cauagagcua gucaccacga cagucucaaa cauggccgaa guaaggucuu      780

acugcuacga agccagcauc uccgauaugg ccucagacuc ccgaugcccc acccaaggag      840

aggccuaccu cgacaaacaa ucagacaccc aauacguaug caaaagaacc cuaguugacc      900

gcggcugggg aaacggcugc ggccucuucg gcaaagggag ccuaguaacc ugcgcaaaau      960

ucacaugcuc caagaaaaug accggcaaau ccauccaacc agaaaaccuc gaguaccgca     1020

uaaugcucuc aguacacggc ucacaacaca gcggaaugau aggcuacgaa accgacgaaa     1080

accgcgccaa agucgaaguc acccccaaca gcccacgcgc cgaagccaca cucggcggau     1140

ucggcucccu aggccuagac ugcgaacccc gcaccggucu ugauuuuuca gaccuuuauu     1200

accucacaau gaacaacaaa cacuggcucg ugcauaaaga augguuucac gauauaccac     1260

uaccguggca ugcgggagca gacaccggca cgccccacug gaacaacaaa gaagcacucg     1320

ucgaguucaa agacgcccau gccaaacgcc aaaccguagu aguucuagga ucacaagaag     1380

gagcagucca caccgcccuc gccggagccu uagaagcaga aauggacgga gcgaaaggca     1440

aacuguucag cggccaccua aagugcagac ucaaaaugga caagcuccgc cucaaaggcg     1500

ucucauacuc acuaugcacc gcagccuuca ccuucaccaa ggucccagca gaaacccucc     1560

acgggaccgu cacugucgaa guccaguaug caggcacgga cggaccaugc aagaucccgg     1620

uccagauggc aguagacaug caaacccuca cacccgucgg ccgacucauc acagcaaacc     1680

ccgugaucac agaauccacu gaaaacucaa aaaugaugcu cgaacucgac ccgcccuucg     1740

gcgacagcua caucgucauc ggcgucggcg acaaaaagau aacccaccac uggcaccgca     1800

gcgggucaac uaucggcaaa gcauucgaag ccaccguccg cggcgccaaa cgaauggccg     1860

uacucggaga cacggccugg gauuucggcu ccguaggcgg aguauucaac agccuaggaa     1920

agggcaucca ccaaaucuuc ggagcagcau ucaaaagccu auucggcggc augucauggu     1980

uuucccaaau acugaucggc acacucuuag ucuggcucgg acucaacacc aagaacggcu     2040

ccaucagccu aaccugccuc gcacuaggcg gagucaugau cuuccuaagc accgccguaa     2100

gcgcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  7335
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 2 opt

<400>  7335
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggaau agucggccuc uuacuaacca cugccauggc cgcagaagua acccgccgag      120

ggagcgcaua cuacauguac cucgaccgca acgacgcagg agaagccauc agcuucccca      180

cgacgcuagg caugaacaag ugcuacauac agaucaugga ccuaggccau augugcgacg      240

ccacaauguc auacgaaugc ccaaugcuag acgaaggcgu cgaacccgac gacgucgacu      300

gcuggugcaa uacaacaagc acuuggguag ucuacggaac cugccaccac aagaaagggg      360

aagcacgacg aagccgccga gcagucacuc uccccucaca cagcacccgg aaacuccaaa      420

cacgcagcca aaccuggcua gaaagccgag aauauacaaa acaccucaua cgaguagaaa      480

acuggaucuu ccgaaaccca ggcuucgcau uagccgccgc ggccaucgcc uggcuacugg      540

gcucgucaac aucacagaaa guuaucuacc uaguaaugau acuucuaauc gcccccgccu      600

acagcauaag augcaucggc guaagcaaca gagacuucgu agaggggaug agcggcggga      660

ccuggguaga cgucguccug gagcacggcg guugcgucac gguaauggcc caagacaaac      720

caaccguuga caucgaacuc guaacgacga ccguguccaa cauggcagaa guccgaucau      780

acugcuacga agccagcauc agcgacaugg cauccgacuc uagaugcccg acccagggag      840

aagccuaccu ugacaaacaa agugacacac aauacgucug caaacgaacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg ggaaaggcag ccuaguaacc ugugcgaaau      960

ucgcaugcag caagaaaaug acagggaaau ccauacagcc agaaaaccua gaauaccgca     1020

uaaugcugag uguccacgga ucucaacaca gcggcaugau cguaaacgac acaggacacg     1080

aaacagacga aaaccgagca aaaguagaga ucaccccgaa cagcccccga gcggaagcaa     1140

cacuaggcgg auucggcagc cugggccucg acugugaacc cagaacaggc uuagacuuca     1200

gcgaccucua cuaccucacc augaacaaca aacacuggcu cguccauaaa gaaugguucc     1260

acgacauccc gcucccaugg cacgccggcg cagacacagg aacgccacac uggaacaaca     1320

aagaggcgcu aguggaguuc aaagacgcac acgccaaacg ccagaccgua gucguacuag     1380

gaucacaaga aggcgccguu cacacagccc uagccggagc acucgaagcc gaaauggacg     1440

gcgcaaaggg cagacuauca ucaggccacc uaaaaugccg ccuaaagaug gacaaacugc     1500

gccuaaaagg cgugucauac ucacuaugca cagcagcauu cacguuuacc aagauaccag     1560

cagaaacacu acacgguaca gucaccgucg aaguccaaua cgcagggacc gacggaccau     1620

gcaaaguacc cgcccaaaug gcaguggaca ugcaaacacu cacaccggua ggacgacuua     1680

ucaccgccaa cccgguaaua acagaaucca ccgaaaacag caaaaugaug cucgaacucg     1740

acccacccuu cggagacucc uacauaguaa uaggcgucgg agaaaagaaa aucacacacc     1800

acuggcaccg cuccggcucc accaucggga aagcauucga agccacaguc agaggagcca     1860

aacgaauggc gguccugggc gacacggcau gggacuucgg aagcgucggg ggcgcacuca     1920

acucacucgg ccguggaaua caccaaaucu ucggggccgc guucaaaagc cucuucggcg     1980

gcaugagcug guucucgcaa auccucauag gcacccuacu cauguggcuc ggacuaaaca     2040

ccaaaaacgg aucaaucagc cucaugugcu uagcccuagg cggcguccuc auuuuucuca     2100

gcaccgcggu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7336
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 2 opt

<400>  7336
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguaggaau cguuggcuua cuccucacca ccgcaauggc agcagaagua acucggcgcg      120

gcucagccua cuacauguac cucgaccgca acgacgcagg agaagccaua agcuucccca      180

ccacccuugg caugaacaaa ugcuacaucc aaauaaugga ccuuggucac augugcgacg      240

cgaccaugag cuacgaaugc cccaugcuag acgaaggagu cgaaccagac gacguagacu      300

gcuggugcaa caccacaagc accugggucg uauacggcac cugccaccac aaaaaaggcg      360

aagccagacg cucacgacga gccgucacuc uucccuccca cuccacccgc aaacuacaaa      420

cgcgauccca aaccugguua gaaagccgcg aauacaccaa acaccuuaua agggucgaga      480

acuggauauu ccgcaauccc ggacuagcuc uugcagccgc agcaauagcc uggcuacuag      540

gaucguccac cucacaaaaa gucauauacc uagucaugau ccuccuaaua gcuccagccu      600

acagcauacg uugcaucggc guuuccaacc gggacuucgu agaaggcaug agcggcggca      660

ccugggucga cguuguacug gaacacggag gaugcgucac cgucauggcc caagacaaac      720

ccacugucga caucgaacua guaaccacaa ccgucagcaa cauggccgag guacgcagcu      780

acugcuacga ggcguccauc agcgacaugg cguccgacuc acggugcccc acccaaggcg      840

aagcauaccu agacaaacaa ucugacaccc aauacguaug caaacgaacc cucguggacc      900

gaggaugggg caacggcugc ggccuguucg gcaaaggcuc acuagucacc ugugcaaaau      960

ucgccugcuc caaaaaaaug acagggaaau ccauacaacc cgaaaaccua gaauauagaa     1020

ucaugcuaag cgugcacggu ucccagcauu ccggcaugau aguaaacgac accggccacg     1080

aaaccgacga aaaccgugca aaagucgaaa uaacuccaaa cucaccccgc gccgaagcaa     1140

ccuuaggcgg cuucggcuca cucggccuag acugcgagcc ccgcaccggg uuagacuucu     1200

ccgaccugua cuaccuuacc augaacaaca aacacuggcu aguacacaaa gaaugguucc     1260

acgacauacc ccuaccaugg cacgcggggg ccgacacagg aaccccccac uggaacaaua     1320

aagaagcccu cgucgaauuc aaagacgccc acgcuaagcg ccaaaccgua gucguccuag     1380

ggucacagga gggagccguc cacacugccc uagccggcgc ucucgaagca gaaauggaug     1440

gcgcaaaagg gcgacuuagc uccggacacc uaaaaugucg ccucaaaaug gacaaauugc     1500

gacuuaaagg agucucuuac ucucucugca ccgccgccuu cacauucaca aaaaucccag     1560

ccgaaacccu ccacggcacc gucaccguag aaguccaaua ugcaggcacc gacggacccu     1620

gcaaaguccc ugcacaaaug gccgucgaca ugcaaacacu cacaccagua ggccgacuca     1680

uaacugccaa cccgguaauc accgaaucca cugaaaacuc gaaaaugaug cucgaacucg     1740

acccaccauu cggagacuca uacauaguca uaggugucgg agaaaaaaaa auaacccauc     1800

acuggcacag aucaggcuca accaucggca aggcauucga agccaccgua aggggcgcaa     1860

aacgcauggc cguccuagga gacaccgcau gggacuucgg aucgguaggc ggcgcacuca     1920

acagccuagg caaaggcaua caccagauau ucggcgcagc cuuuaaaagc cucuucggcg     1980

gaaugucaug guucagccag auccucauag gcacacuccu uauguggcua ggccuaaaca     2040

caaaaaacgg auccaucucc cucaugugcc ucgcccucgg aggaguacug aucuuccuau     2100

cgaccgcagu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7337
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 2 opt

<400>  7337
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguagguau agucggccuc cuccucacca ccgcaauggc agccgagguc acacgcagag      120

gcagcgcaua cuacauguac cuagaccgga acgacgcagg agaagcaauc ucguucccaa      180

ccacccuugg gaugaacaaa ugcuacauac agauaaugga ccugggacau augugcgacg      240

ccacaauguc cuacgaaugc ccaaugcucg acgagggggu agaacccgau gacguagacu      300

gcuggugcaa caccacaagc acaugggucg ucuacggcac augccaccac aagaaagggg      360

aagcccggag gagcagacga gcagucacac uccccagcca cagcacaaga aaacuacaga      420

cccgcucaca aaccuggcua gaaagccgag aguacacaaa acaccucaua cgaguugaaa      480

acuggaucuu ccgcaacccc ggauucgcgc uagccgcggc agcaauagca uggcuccugg      540

gaucuagcac cagucaaaaa guuaucuacc uugucaugau acuccuaaua gcccccgcau      600

acagcauacg augcauaggc guaagcaacc gcgacuucgu cgaagggaug ucaggaggga      660

ccugggucga cguaguacua gaacacggcg gaugcgugac agucauggcc caagacaaac      720

ccacaguaga cauagagcuc gucacaacca caguauccaa cauggccgaa guccgcucgu      780

acugcuacga agcaucaauc agcgacaugg ccucagacuc ccgcugcccc acgcagggag      840

aagcauaccu cgacaaacaa ucagacacac aguacgucug caaaaggacc cuaguagacc      900

gaggaugggg gaacgggugc ggacucuuug gaaagggauc gcucguaaca ugcgcaaaau      960

ucgccugcag caaaaagaug acaggcaaau caauacagcc ggaaaaccuc gaauaccgga     1020

ucaugcucuc cguacacggc ucacaacacu cgggcaugau agucaacgac acaggccacg     1080

aaacagacga aaacagggcc aaagucgaaa ucaccccgaa cucaccgcgc gcagaggcua     1140

cccuaggagg guucggaucc cucggccuag acugcgaacc gcgaacagga cuggacuuca     1200

gugaccucua cuaccuaacc augaacaaca aacacuggcu aguccacaaa gaaugguucc     1260

acgacauacc ccuccccugg cacgcaggag ccgauaccgg cacgccgcac uggaacaaca     1320

aagaggcccu agucgaguuc aaagacgcac acgcaaaaag gcaaacgguc gucguacucg     1380

gcucgcaaga aggagcagua cauacagcac uagcaggcgc acucgaggcc gaaauggacg     1440

gagccaaagg gcgccuuucc agcggccacc ucaaaugccg gcuuaaaaug gacaaacuua     1500

ggcuaaaagg ggucuccuac agccucugca ccgccgccuu cacauucacu aaaaucccag     1560

cagagacccu acacggaaca gugaccguag aagugcaaua cgccgggaca gacggacccu     1620

gcaaaguacc cgcacaaaug gccguugaca ugcagacacu aacccccguc ggacgacuaa     1680

ucaccgccaa uccggugaua accgaaucga cugaaaacag caaaaugaug cucgaacucg     1740

acccacccuu cggcgacagc uacaucguaa uaggagucgg cgaaaagaaa auaacccacc     1800

acugguaccg cuccggaagc accaucggca aagcguucga agccacgguc agaggugcaa     1860

aacgcauggc cguccuaggc gauacggccu gggacuucgg aagcguuggg ggagcacuaa     1920

acucacuagg caaagggaua caccaaauau ucggugccgc auucaaaucc cuguucggag     1980

gaaugucaug guucucacaa auacuaauag gcacacuacu aauguggcug ggccucaaca     2040

ccaagaacgg aagcauuucc cuaaugugcc ucgcccucgg cggcguacua aucuuccuau     2100

ccacagcagu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7338
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 2 opt

<400>  7338
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacgu       60

cuguaggcau aguuggccua cuccucacaa cagccauggc agccgaagua acccgccgcg      120

gcucagccua uuacauguac cuggaccgaa acgacgcugg agaagccauc ucauucccca      180

ccacccucgg aaugaacaaa ugcuacauac aaaucaugga ccucggacac augugcgacg      240

cgaccaugag uuacgaaugc ccaauguugg acgaaggagu cgagcccgac gacguugacu      300

gcugguguaa caccaccagu acuugggucg ucuacggaac cugccaccac aagaaagggg      360

aagcccgacg aucccgccgg gcgguaaccc uaccuuccca cagcacccgc aaacuccaaa      420

cacgaucaca aaccuggcuc gaaagcagag aauacacgaa acaucucaua cgaguagaga      480

acuggauauu cagaaauccg ggauucgcac uagccgccgc cgccaucgcc uggcuccugg      540

gaucaucaac cagccaaaaa guaauauacc ucgugaugau ccuccuaaua gcccccgcgu      600

acucaauacg cugcauaggc gucagcaacc gggacuucgu agaaggcaug uccgggggca      660

ccugggucga cguaguacuc gagcacggcg gcugcgugac cguaauggcc caagacaaac      720

ccaccguaga caucgaacug guuacaacca cagugucaaa cauggcagaa gucagaagcu      780

acugcuacga agccucgaua agcgacaugg ccuccgacag ccgaugcccc acccaaggag      840

aagccuaccu agacaaacaa ucagacacac aauacgucug caaacgaacc cuaguagacc      900

gcggcugggg caacggaugc ggccuuuucg gaaaaggcuc acuuguaacc ugcgccaaau      960

ucgcaugcag caagaaaaug accggaaaau caauccaacc agaaaaccua gaguaucgca     1020

ucaugcucuc cguacacgga ucacaacacu caggcaugau agucaaugau acgggccacg     1080

aaaccgacga aaauagagca aaagucgaga ucacccccaa cagcccccgc gccgaagcaa     1140

cacucggagg auucgggagc cucggacucg acugcgaacc ccgaacaggc cuagacuucu     1200

cagaccuaua cuaccucacc augaacaaca aacacuggcu cguacacaaa gaaugguuuc     1260

acgacauacc acuacccugg cacgcugggg ccgacaccgg gacuccccac uggaauaaca     1320

aagaagcccu aguagaguuc aaagacgccc acgccaaacg ccaaacgguc guaguacucg     1380

gcagccaaga aggggccguc cacaccgcgc ucgccggagc ccucgaagcc gaaauggacg     1440

gagcaaaggg acggcuaucc ucagggcacc ucaaaugcag acuaaagaug gacaaacucc     1500

gucugaaggg cguuuccuau agccucugca ccgcagcauu caccuucaca aagaucccgg     1560

ccgaaacacu ccacgggacc gucaccguag aagugcaaua cgccggcaca gauggccccu     1620

gcaaaguccc cgcccagaug gccgucgaca cccaaacgcu gacacccguc ggccgccuaa     1680

ucaccgcgaa ucccgucaua acugaaucca ccgaaaacuc gaaaaugaug uuagaacucg     1740

aucccccauu cggggacuca uacaucguaa uaggcgucgg agaaaagaaa auaacccacc     1800

acuggcaccg aucaggcagc accauaggca aagccuucga agccacugua aggggcgcaa     1860

aacgcauggc aguacuagga gacaccgcau gggacuuugg cucagucggc ggagcccuca     1920

acucccuagg caaaggaaua caccagauuu ucggagcagc uuucaaaagc cucuucggcg     1980

gcaugagcug guucucccag auucuaauag gcacccugcu cauguggcuc ggacugaaca     2040

ccaaaaacgg auccaucucc cucaugugcc uagcacuggg cggcguccua auauuccugu     2100

cuaccgccgu auccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7339
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 2 opt

<400>  7339
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

ccgugggcau agucggucuc cuacuuacca cagcaauggc agccgaagua acacgccgag      120

gcucagcaua cuacauguac cucgaccgga acgacgcagg cgaagcaaua uccuucccaa      180

ccacacucgg caugaacaaa ugcuacaucc aaaucaugga ccucggccac augugcgacg      240

ccacaauguc cuacgaaugc cccaugcucg acgaaggggu ggaaccagac gacgucgacu      300

guuggugcaa caccaccucc accugggucg ucuacgggac cugccaccac aagaaaggcg      360

aagcccgccg aagcagacgc gcagucacac ucccuuccca cucaacccgc aaacugcaaa      420

cccgcucaca aaccuggcuc gaaagccgcg aauacaccaa acaccuuaua agaguagaaa      480

acuggaucuu ccgaaaccca ggcuucgcac ucgccgcagc cgccauagcc uggcuacuug      540

ggagcuccac gucccaaaaa guaauauacc ucguaaugau ucuccuaaua gcgccagccu      600

acuccauccg gugcauagga gucucaaaca gagacuucgu cgaagguaug ucgggaggaa      660

ccugggucga cguaguacua gaacacgggg gcugcgucac ugucauggca caagacaaac      720

cgaccgucga cauagaacuc guaacaacca ccgugucuaa cauggccgaa auccgaagcu      780

acugcuacga agcaagcaua agcgacaugg ccuccgacuc gcgaugccca acccaaggag      840

aagccuaccu ugacaaacaa agugauacac aauacgucug caagagaaca uuaguagacc      900

gaggaugggg gaacggaugc ggccuauucg gcaaaggcuc acucgucacg ugcgccaagu      960

ucgcuugcuc caagaaaaug accggcaaaa gcauacaacc ugaaaaccuc gaauacagaa     1020

uaaugcuauc cguccacggc ucccaacaca gcggaaugau cgucaacgac acaggacacg     1080

aaacagacga aaaccgcgcc aaaguagaaa ucacccccaa cucaccccga gcagaagcca     1140

cucucggcgg cuucggcucc cuaggacuag acugcgaacc ccgaaccggc cuggacuuuu     1200

ccgaccucua cuaccugacc augaauaaca aacacuggcu aguacacaaa gaaugguucc     1260

acgacauacc cuuacccugg cacgccggcg ccgacaccgg cacaccccac uggaacaaca     1320

aagaagcccu ggucgaguuc aaggacgcac acgcaaaacg ucaaaccgua guaguccucg     1380

gcucccaaga aggcgccguc cacaccgccc uagcaggggc acuagaagca gaaauggacg     1440

gggccaaagg aagacuaucc agcggccacc ucaaaugccg acucaaaaug gacaaacuca     1500

gacucaaagg aguaucauac agccuaugua ccgcagcguu caccuucacc aaaaucccag     1560

ccgaaacacu acacgggaca guaaccguag aaguacaaua cgccggaacu gacggacccu     1620

gcaaaguacc cgcacaaaug gcuguggaca ugcaaaccuu aaccccagug ggcagacuga     1680

ucacagccaa ccccguaaua acggaaucga cggaaaacuc caaaaugaug cucgaacuag     1740

acccucccuu cggcgacuca uacaucguca ucggcgucgg cgagaaaaag aucacccacc     1800

auuggcaccg aagcggcagc acgauaggca aagcauucga agcaacagua agaggcgcca     1860

aacgaauggc cguccuagga gacaccgcau gggacuucgg cagcguagga ggcgcacuaa     1920

acucacucgg aaaaggaauc caccaaaucu ucggagcagc cuucaaaucc cuauucgggg     1980

gcaugaguug guucucccaa auacucaucg gcacccuacu uauguggcuc ggccuuaaca     2040

ccaagaacgg aagcauaagc cugaugugcc ucgcccuggg cggcguccuc aucuuccuuu     2100

cuaccgcagu aucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7340
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 2 opt

<400>  7340
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggaau cgucggacua cuccuaacaa ccgcgauggc ugccgaagua acccgacgcg      120

ggagcgccua cuacauguac uuagacagaa acgacgcagg ggaagcaauc agcuucccua      180

caacgcucgg aaugaacaaa ugcuacauac aaauaaugga ccucggacac augugcgacg      240

caacaaugag uuacgaaugc ccaaugcuag acgaaggcgu agaaccagac gacguagacu      300

gcuggugcaa caccacgagc acguggguag ucuacgguac gugucaccac aagaaaggcg      360

aagcacgacg cuccaggaga gccguaacgc ucccgagcca cucgacacga aaacuccaaa      420

cacgcagcca aaccuggcua gaaucacgag aauacacgaa gcaccuaauc cgaguagaaa      480

acuggauauu ccgaaacccc ggauucgcgc ucgccgccgc ggccaucgca uggcuccuug      540

ggagcagcac aagccaaaaa gugauauacc uaguaaugau ccuacucauc gccccagcau      600

acaguauacg augcaucgga gugagcaacc gcgacuucgu ggaaggaaug uccgggggca      660

ccugggucga cgucguccug gaacacggcg gaugcgucac ggucauggca caagacaaac      720

caacagugga cauagaacuc gucaccacca cagucagcaa cauggcagaa guccgcagcu      780

acugcuacga agccagcauc ucagacaugg ccuccgacag cagaugcccc acccaaggcg      840

aagcguaccu agacaaacag uccgacacgc aauacgucug caaacggaca cucguagaca      900

gaggaugggg aaacggcugc ggccuguucg gcaaaggcuc ccuagucacc ugugcaaagu      960

ucgccugcag caagaaaaug acaggcaaau caauccagcc ggaaaaccuc gaauacagaa     1020

uaaugcucag cguccacggc ucccaacaua gcggcaugau aguaaacgac acgggccacg     1080

aaacagacga aaaccgagcc aaagucgaaa uaacacccaa cagcccccgc gcggaagcca     1140

cacucggagg cuucgggucg cuaggacucg acugugaacc aagaacaggc cuggacuuca     1200

gcgaccucua cuaccucacc augaacaaca aacacuggcu ugugcauaaa gaaugguucc     1260

acgacauacc acucccgugg caugccggag cagacaccgg aacaccacac uggaacaaca     1320

aagaagcccu aguggaguuc aaagacgcac acgcaaaacg acaaacagua gucguacuag     1380

gaucccaaga aggcgcugua cacaccgcac uagccggcgc acuagaagcc gaaauggacg     1440

gagcaaaagg acgccuaucc ucaggccacc ucaagugcag gcuaaagaug gacaaacucc     1500

gauuaaaggg agucuccuac agccugugca ccgccgccuu caccuucacc aaaaucccag     1560

ccgaaacacu gcacgggacc gugacaguag aaguacaaua cgccggcaca gauggacccu     1620

gcaaaguacu agcacaaaug gccgucgaca ugcaaacacu aaccccaguc ggccgccuca     1680

uaacggcaaa ccccgugauc acagaaucca cagaaaacuc gaaaaugaug cuagaacucg     1740

acccacccuu cggcgacuca uacauaguaa ucggaguagg agaaaagaaa auaacccacc     1800

acuggcacag aagcggaagc acaauaggca aagccuucga agcaaccguc cgaggagcca     1860

aaagaauggc agucuuaggc gacaccgcau gggacuucgg gagcgugggc ggagcacuaa     1920

acagcuuggg aaaaggaauc caccaaauau ucggagcagc guucaaaagc cuguucggag     1980

gcaugaguug guucagucag auccucaucg gcacacuccu cauguggcua ggacuaaaca     2040

ccaaaaacgg cagcaucuca cugaugugcc ucgcgcucgg aggcguacug auauuccuga     2100

gcaccgccgu auccgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7341
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 2 opt

<400>  7341
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

cagucgggau ugucggacuc cuacucacca cagccauggc agccgaggug acccgacgag      120

gaucagccua cuacauguau cucgaucgca acgacgcagg cgaagccaua ucguucccca      180

caacccucgg caugaacaaa ugcuacaucc aaaucaugga cuuaggccau augugcgacg      240

ccaccauguc auacgaaugc cccacacuag acgaaggcgu cgaacccgac gacgucgacu      300

gcuggugcaa uaccaccucc acaugggucg uauacggcac augccaccac aagaaaggcg      360

aagcccgccg aucccgcaga gccguuaccu uaccaagcca cuccacccga aaacuccaaa      420

cgcggagcca aaccuggcua gaaagccgcg aauacacaaa acaccucauc cgcgucgaaa      480

acuggauauu ccgcaaccca ggauucgccc ucgccgccgc cgcaauagca uggcugcucg      540

gcuccagcac aucccaaaaa gucaucuacc ucguaaugau ccuucucauc gcccccgccu      600

acuccauccg cugcaucggc gucucaaacc gagacuucgu cgaaggcaug ucaggcggca      660

ccugggucga cgucguccug gaacacggag gcugcguaac cgucauggcc caagacaaac      720

cgacaguaga caucgagcua gugaccacaa cagucagcaa uauggccgaa gucagaucuu      780

acugcuauga agcguccauc ucugacaugg ccuccgacuc acgcuguccc acgcaagggg      840

aagccuaccu ggacaaacaa agcgacacgc aguacgucug uaaacgcacc cuagucgacc      900

gcggcugggg caacggaugc ggacuauuug gaaaaggcuc acucgucacu ugcgccaaau      960

ucgccugcuc aaagaagaug accggaaagu cuauccaacc ggaaaaccua gaauaccgca     1020

uaaugcuauc cguccacggg ucacaacacu caggaaugau agucaaugac accggacacg     1080

aaaccgacga aaaccgugcu aaaguagaaa ucacccccaa cuccccuaga gccgaagcaa     1140

cccuaggggg auucggaagu cuaggccugg acugcgaacc acgcaccggc cuggacuucu     1200

ccgaccucua cuaccucaca augaacaaca agcacuggcu aguccacaaa gaaugguucc     1260

acgacauccc acuaccuugg cacgccggcg ccgacacagg cacaccccac uggaacaaca     1320

aagaagcccu cguagaguuc aaagacgcuc acgccaaacg ucaaaccguc gucguccuag     1380

ggagccaaga aggcgcaguc cacaccgcgu uggcaggcgc ccuagaagca gaaauggacg     1440

gagcaaaagg acgacucucc ucgggccacc uaaaaugccg gcuaaaaaug gacaaacuac     1500

gccucaaagg cgucuccuac agccucugca ccgcagccuu caccuucacc aagaucccag     1560

ccgaaacccu ucacggcacc guaaccguag aaguccaaua cgcaggcaca gacggcccau     1620

gcaaaguacc agcccaaaug gcaguagaua ugcaaacacu aaccccuguu ggccgccuca     1680

ucacagccaa ccccguaauc accgaaagca ccgagaacuc aaaaaugaug cuagagcucg     1740

acccacccuu uggcgauucc uacaucguca ucgggguagg cgaaaagaaa aucacgcacc     1800

acuggcacag guccggaagc acaaucggca aagcguucga ggcaacuguc cgaggcgcca     1860

aacgaauggc aguccucggu gacaccgccu gggauuucgg aagcgucggc ggugcccuca     1920

auagccuagg caagggcauc caccaaaucu ucggcgccgc cuucaaaucc cuguucggag     1980

gcaugucaug guucucacag auccuaauag gcacccugcu aauguggcua ggccucaaca     2040

ccaaaaacgg aucgaucucc uuaaugugcc uagcacucgg cgggguccua aucuuccuau     2100

ccacagccgu aucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7342
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 2 opt

<400>  7342
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

cgguagggau cgucggccua cuccuaacaa cagccauggc cgccgaaguc acccgccgag      120

gaagugccua cuacauguac uuagaccgaa acgacgccgg ggaggccaua ucguucccca      180

caacacuagg aaugaacaaa ugcuacauuc agauaaugga ccuaggacac augugugacg      240

caaccaugag uuacgaaugu cccaugcugg acgaaggagu agagcccgac gacguagacu      300

gcuggugcaa cacaaccagc acuuggguag ucuacggaac gugucaucac aagaaaggcg      360

aagcacgacg aucccgaagg gcuguaacgc ugccaucaca cucgacccga aaacuucaaa      420

cacgaucgca aacauggcua gagucccgcg aauacacaaa acaccucaua cgcgucgaaa      480

acuggauauu ccgcaacccc ggcuucgccc uggccgcagc cgccaucgca uggcuccucg      540

gcagcaguac cucccaaaaa gucaucuacc ucguaaugau ccuacuaauc gccccagcau      600

acagcauacg augcaucgga guaucaaacc gcgacuucgu cgaaggcaug agcggcggaa      660

cauggguaga cguaguauua gaacacggag gcugcguaac uguaauggcg caagacaaac      720

cgacaguaga caucgagcua gucaccacca ccgucaguaa cauggcagaa guccggucau      780

acugcuacga agccagcaua ucagacaugg cguccgacag ccggugcccc acccagggcg      840

aagccuaccu agacaaacaa uccgacaccc aauacgugug caaaagaacc cuaguagaca      900

gaggaugggg caacggaugc ggccuauucg ggaaaggguc gcucguaaca ugcgcaaaau      960

ucgccugcuc aaagaaaaug acagggaaau caauccaacc agaaaaccua gaauacagaa     1020

ucaugcuauc uguccacggc ucccagcaca gcggcaugau agucaacgac acaggccacg     1080

aaaccgacga aaacagggcc aaaguagaaa ucacaccaaa cuccccacgc gccgaagcaa     1140

cauuaggcgg cuucggcagc cuaggccucg acugcgagcc ccgaacgggc cuugacuucu     1200

ccgaccuaua cuaccucacc augaacaaca aacacuggcu uguacacaaa gagugguucc     1260

acgacauacc ccuacccugg cacgcaggcg ccgacacagg cacaccccac uggaacaaca     1320

aagaagcccu ggucgaguuc aaagacgcac acgcaaaacg ccaaacagua guuguacucg     1380

gaagccagga aggcgcugua cacacagccc ucgcaggagc ucuggaagcc gaaauggacg     1440

gugcaaaagg ccgacucuca agcggccacc ugaaaugcag acuaaaaaug gacaaacuca     1500

gacucaaagg ggugagcuac agccucugca cagccgcauu cacauucaca aaaauaccag     1560

ccgaaacccu ucacgguaca gucacagugg agguccaaua cgcaggaacg gacggaccau     1620

gcaaagugcc cgcccaaaug gcagucgaca ugcaaacccu cacgcccgua gggaggcuca     1680

uaacagcuaa ccccguaaua acagagagca cggaaaacag caaaaugaug cucgagcucg     1740

acccgcccuu uggggacagc uacauaguca ucggcguagg agaaaagaaa aucacacauc     1800

acuggcaccg aagcggcuca acaauaggca aagcguucga agcaacagua cgaggcgcca     1860

aaagaauggc ugugcucgga gacaccgccu gggacuucgg uuccguaggc ggagcacuca     1920

acucucuggg caaaggcaua caccaaaucu ucggagcagc cuucaaaucc cucuucggag     1980

gaaugucaug guucagccaa auccucauag gaacacugcu cauguggcuc ggacuaaaca     2040

ccaaaaacgg cagcauaucc cuaaugugcc uggcacuagg aggcguccua auauuccucu     2100

cuaccguggu aucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7343
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 2 opt

<400>  7343
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguaggcau cgucgggcua cuacuaacaa ccgcgauggc cgccgaaguc acacgcagag      120

gguccgccua uuacauguac cuagacagga acgacgccgg cgaagcaaua ucauucccga      180

ccacgcuggg gaugaacaaa ugcuacauac agauaaugga ccucgggcac augugcgacg      240

caacaaugag uuacgaaugc cccaugcugg acgaaggcgu agaaccggac gacgucgacu      300

gcuggugcaa cacaacgagu gcaugggugg ucuacggaac cugccaccac aagaaaggcg      360

aagcgcgcag aagccgccga gcagucacac uaccaagcca caguacacgu aagcuccaaa      420

caagaccaca aacauggcuc gagucccgag aguacacaaa acacuuaaua cgaguagaga      480

acuggaucuu cagaaaccca ggauucgcac ucgcagccgc agcaaucgca uggcuacugg      540

guuccagcac cagccaaaaa gucaucuacc ucguaaugau acuccuaauc gccccggccu      600

acuccaucag gugcaucgga guaagcaacc gcgauuucgu cgaagggaug ucgggaggaa      660

ccuggguaga cgucguacuc gaacacggcg gcugugucac gguaauggcc caagacaaac      720

caacuguaga caucgagcua guaaccacga ccguaucaaa cauggccgaa gucagaagcu      780

acugcuacga agcaucaauc agcgacaugg ccuccgacag cagaugcccc acccaaggcg      840

aagccuaccu ggacaaacaa agcgacacac aauacguaug caagagaaca cuggucgacc      900

gaggaugggg caacggcugc ggccucuucg guaaaggguc gcuaguuaca ugcgccaaau      960

ucgcgugcag caagaaaaug accggaaaaa gcauccaacc agaaaaccuc gaauacagaa     1020

ucaugcuauc cguacacggc agccagcaca gcggcaugau aguaaacgac accgggcacg     1080

aaacagacga aaacagggca aaagucgaga uaacacccaa cucuccacga gccgaagcaa     1140

cccucggagg auucgggagc cucggacuag acugugaacc gaggaccggc cuagacuuca     1200

gcgaccugua cuacuuaaca augaauaaca aacacuggcu uguacacaaa gagugguucc     1260

acgacauccc ccuaccaugg cacgccggcg cagacaccgg aacgccgcac uggaacaaua     1320

aagaggcccu aguagaguuc aaagacgccc acgcaaaacg acaaacugua gucguccuag     1380

gcucacaaga gggagcaguc cauaccgccc uagccggagc gcucgaagca gagauggacg     1440

gagcgaaagg gcgccuauca agcggacacc uaaagugcag gcuaaaaaug gauaaacugc     1500

gccucaaggg cgucagcuac ucacucugca cagcagcauu cacauucaca aaaaucccgg     1560

ccgaaacccu acacggaacc guaacaguag aaguacagua cgcaggaacc gacggacccu     1620

gcaaaguccc ggcccaaaug gcgguugaca ugcaaacccu aacgcccgua ggacgccuga     1680

ucacagccaa ccccguaauc acagaaucaa ccgaaaauuc aaagaugaug cuggaacugg     1740

acccgccauu cggagacagc uacaucguaa uaggggucgg cgaaaagaaa auaacacacc     1800

acuggcaccg auccggaagc accauuggaa aagcauucga ggccacaguc aggggagcga     1860

aacgaauggc aguccucggg gauacagcau gggacuucgg auccguaggc ggagcccuga     1920

acucacuggg caaaggaauc caccaaauau ucggagcagc guucaaauca cuauucggag     1980

gcaugagcug guucucacaa auacuaauag gcacacuacu aauguggcuc ggccuaaaca     2040

cgaaaaacgg cagcaucucc cuaaugugcc uggcccuagg aggcguacua auauuccuau     2100

caacagccgu uuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7344
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 2 opt

<400>  7344
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cagucggaau cgucggccuc uugcuuacaa cagccauggc cgcggaggua acacgccggg      120

gcagcgccua cuacauguac cuagacagaa acgacgccgg cgaagcaauc agcuucccca      180

ccacacucgg uaugaacaaa ugcuacaucc aaaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgaaugc cccaugcuag acgaaggcgu cgaacccgac gacguagacu      300

gcuggugcaa caccaccagc acaugggugg ucuacggaac cugccaccac aagaaaggag      360

aagcccgaag gagccgccgc gcagucaccc uacccagcca cucaacaaga aaacugcaga      420

cacgaagcca aacauggcua gaaucccgcg aauacacaaa acaccuaauc agagucgaaa      480

acuggaucuu cagaaaucca ggcuucgcac ucgccgcugc agcaauagcg uggcuacucg      540

gcagcucaac aucaaagaaa guaaucuacc uaguaaugau ccuacugaua gcaccagccu      600

acagcaucag augcaucggg guauccaaca gagacuucgu cgaaggcaug agcggaggga      660

cguggguaga cgucguccuc gaacacggcg gaugcgucac cguaauggca caagacaaac      720

caacagucga cauagaacuc guaaccacaa ccgucucgaa cauggccgaa guccgaagcu      780

acugcuacga agcgagcauc agcgacaugg caucagacuc acggugccca acccaaggag      840

aagcauaccu agacaaacag agcgacaccc aauacgucug uaaaagaacc cucgucgacc      900

gcggaugggg caacggcugc ggccuauucg gaaaaggauc acucguaacc ugcgccaaau      960

ucgccugcuc caagaaaaug acaggaaaaa guauucagcc cgaaaaccuu gaguaccgca     1020

ucaugcuguc gguccacgga agucaacaca gcggcaugau cguaaacgac acuggacacg     1080

agaccgacga aaaucgagca aaagucgaaa uaacaccgaa cucaccccgc gccgaagcca     1140

cccuuggcgg auucggaucc cucggccuag acugcgaacc cagaaccggc cuagacuucu     1200

cggaccucua uuaucucacg augaacaaca aacacuggcu uguccacaaa gaaugguucc     1260

acgacauucc acuacccugg cacgcaggag cagacaccgg caccccacac uggaacaaca     1320

aagaagcccu aguggaguuc aaagacgccc acgccaaacg ccagacagua guaguccuag     1380

gcucccaaga aggagccguc cacacagccc uggccggcgc acuagaagcc gaaauggacg     1440

gggcgaaagg gcgccuuagu agcgggcauc uuaaaugccg ccuaaaaaug gacaaacuca     1500

gacucaaagg agucagcuau ucccucugca cugcagcauu caccuucacu aaaaucccag     1560

ccgaaacccu acacggcacc guaaccgugg aaguacaaua cgcgggcaca gacggcccau     1620

gcaaaguacc cgcccaaaug gccguagaca ugcaaacccu cacaccagug ggcaggcuca     1680

uaacagccaa ccccgucauc acggaaucga ccgagaacag uaaaaugaug cuggaauuag     1740

acccgcccuu cggagauagc uacaucguaa ucggaguagg agagaagaaa aucacacacc     1800

acuggcaccg aucaggcagc acaaucggca aggcuuucga ggccaccgua agaggcgcga     1860

aacgcauggc aguacuaggc gacacagccu gggacuucgg cucaguugga ggcgcccuaa     1920

acagccuagg caaaggcauc caccaaaucu ucggcgccgc auuuaaaagc cuauucggcg     1980

gaauguccug guucagccag auccugaucg gaacucuccu aauguggcug ggguuaaaca     2040

ccaaaaacgg gucgaucuca cuaaugugcc ucgcacuagg cggaguacua aucuuccuga     2100

gcacagcagu cagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7345
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 2 opt

<400>  7345
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

ccgucgggau cgucggccuc cuacugacaa ccgcuauggc cgccgaaguc acacgcagag      120

gauccgcuua uuacauguac cucgaccgaa acgacgcagg cgaagcgauc aguuucccca      180

caacccucgg aaugaacaaa ugcuacauac aaaucaugga ccucggacac augugcgaug      240

cgacgauguc cuacgaaugc cccauguuag acgaaggcgu cgaacccgac gauguagacu      300

gcuggugcaa cacaaccagc accuggguag uauacggcac augccaccac aagaaaggag      360

aggcccgcag aucacgcaga gcagucacac uacccaguca cagcacgaga aaacuccaaa      420

caaggagcca gacauggcuc gagagccggg aauacacaaa acaccuaaua cgaguagaaa      480

acuggaucuu ccgaaaccca ggauucgcgc ucgccgccgc cgccaucgcc uggcuccucg      540

gcagcagcac cagacagaaa guaauauacc ucgucaugau ccuccuaaua gcaccagcau      600

auucaauacg augcaucggg guaagcaacc gcgacuucgu ugaagggaug uccggcggaa      660

cauggguaga ugucguccuu gaacacggcg gcugcguaac cguaauggcc caagacaaac      720

ccacgguaga cauagagcuc guuacaacaa ccguauccaa cauggccgaa gucaggagcu      780

acugcuacga ggccagcaua uccgacaugg ccagcgacag cagauguccc acacaaggag      840

aagcguaccu cgacaaacaa agcgacacac aauacguaug caaacgaacc cuagucgaca      900

gaggcugggg aaacggaugc ggccucuucg gcaagggcuc ccucguaacc ugugcaaagu      960

ucgccugcuc caagaagaug accggcaaaa gcauacaacc cgaaaaccua gaauaucgaa     1020

ucaugcuaag cguacacggg agccaacacu caggcaugau cgucaaugac acaggccacg     1080

aaacagacga aaacagggcc aagguagaaa ucacccccaa cagcccccgc gccgaagcca     1140

cccugggcgg auucggcagc cuaggccuag acugcgaacc cagaacagga cuagacuucu     1200

ccgaccucua cuaccucaca augaacaaua aacauuggcu gguacacaag gaaugguucc     1260

acgacauacc ccucccuugg cacgccggag cagacaccgg aacuccacac uggaacaaca     1320

aagaagcccu aguggaguuc aaggacgcac acgcaaagag acaaacgguc guaguacugg     1380

gaucacaaga aggcgcaguc cacaccgccc ucgcgggagc acuagaagcc gaaauggacg     1440

gcgcaaaagg ccgacuaucc agcgggcacc uaaaaugcag acuaaaaaug gacaaacucc     1500

gacuaaaagg agucaguuac agccuaugca ccgccgcauu cacguucaca aaaaucccgg     1560

ccgagacgcu gcacggcacc gucacgguug aagugcaaua cgccggcaca gacggccccu     1620

gcaagguucc cgcccaaaug gccguagaca ugcaaacgcu aaccccaguc ggccgccuua     1680

uaacggccaa ccccguaauc accgaaucaa cagaaaacuc caaaaugaug cucgaacuag     1740

acccaccauu cggcgacagc uacaucguaa ucggcgucgg cgaaaagaaa auaacgcacc     1800

acuggcaucg cagcggaagc acaaucggca aagcguucga agccaccgua cgcggagcca     1860

aacgcauggc cgugcucggc gacacagccu gggacuucgg aucagucggc ggagcccuaa     1920

acagccucgg caaaggcaua caccaaauau uuggagcggc cuucaaaucc cuauucggag     1980

ggaugagcug guucucccaa auccugaucg gaacccuccu cauguggcuc ggacucaaca     2040

cgaaaaacgg cagcauaagc cuaaugugcc uggccuuggg cgguguacuc auauuucuaa     2100

gcaccgccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7346
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 2 opt

<400>  7346
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

guguagggau aguaggccua cuccucacaa cggccauggc agcagaaguc acccguagag      120

gaagcgccua uuacauguac cuagaccgaa acgaugcggg cgaggccauc agcuucccaa      180

ccacucuagg caugaauaaa ugcuacauac aaaucaugga ccucggacau augugcgacg      240

cgacaaugag uuacgaaugc cccaugcuag acgagggcgu agaaccagac gacguggacu      300

gcuggugcaa uacaaccucc acaugggucg ucuacggaac augccaccac aagaaaggcg      360

aggcccgacg cucgcgccgc gcuguaacau uacccucgca cuccacccga aaacuccaaa      420

cacggagcca aacguggcuc gaaagcagag aauacacaaa acacuuaaua agaguagaaa      480

acuggauauu ucgaaacccc ggcuucgccc uagccgcggc agcgaucgca uggcuccuug      540

gaagcagcac aagccagaaa gucauauacc uagugaugau ccuccucauc gcgcccgcau      600

acucaauccg gugcauaggc gucagcaaca gagacuucgu agaaggaaug agcggcggca      660

ccugggucga cguagugcuc gagcacggcg gaugcgucac cguaauggca caggacaaac      720

caaccgucga caucgaacuc gucacaacca cggucucaaa cauggccgaa gucagaucgu      780

acugcuacga ggccuccauc agcgacaugg ccagugacuc cagaugcccc acgcaagggg      840

aagccuaccu cgacaaacaa ucagacacac aauacguaug caaacgaaca cucgucgaca      900

ggggaugggg aaacggcugc ggccucuucg guaaaggauc gcugguaacc ugcgccaaau      960

ucgccugcag caagaaaaug accgggaaaa gcauccaacc ugaaaaccua gaauaccgca     1020

uaaugcucuc aguccacggg ucccagcaca gcggcaugau agucaacgac acaggccacg     1080

aaaccgacga aaacagggcc aaaguagaaa ucacccccaa cagcccacgc gccgaagcaa     1140

cgcuaggcgg cuuuggaucc cucggacuag acugugaacc acgaaccggc cucgacuucu     1200

ccgaccuaua cuaucucacc augaauaaca agcacuggcu cgugcacaaa gaaugguucc     1260

acgacauccc acucccaugg cacgccggcg ccgacaccgg aacgccacau uggaacaaca     1320

aagaagcccu aguggaguuc aaagacgcac acgcaaaaag gcaaacaguc gucguacucg     1380

gaacucaaga aggagcaguc cacacagcgc ucgcaggagc acucgaagca gaaauggacg     1440

gggcaaaggg cagacuaucc agcggacacc ucaagugccg gcucaagaug gacaaacuac     1500

gccuaaaagg cguaagcuac agccuaugca ccgcagccuu caccuucacc aagauacccg     1560

cagaaacccu acacggcaca gucacagugg aaguccaaua cgccggcacc gacggacccu     1620

gcaaaguccc cgcccaaaug gcagucgaca ugcagacgcu cacacccgua ggacguuuaa     1680

ucacagcgaa ccccguaaua accgagucaa ccgaaaacag caaaaugaug cuagaacucg     1740

acccaccauu cggcgacagu uacauaguca ucggcguagg cgagaaaaag auaacccacc     1800

acuggcaccg aagcgggagc acaauaggca aagccuucga agcaaccguc cgaggagcca     1860

aaagaauggc cguccuagga gacaccgccu gggauuucgg gagcgucggc ggggcacuca     1920

acagccucgg aaaaggcaua caccaaauau ucggggcagc auucaagagc uuguuuggag     1980

ggaugucaug guucucccag auccucaucg gcacccuacu cauguggcua ggacuaaaca     2040

ccaaaaacgg gucgaucuca cucaugugcc uagcccucgg gggcguacuu aucuuccuau     2100

caacagcagu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7347
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 2 opt

<400>  7347
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gcgucggcau cguaggacua cuccucacua ccgccauggc agccgaaguc acaagacgag      120

gauccgccua cuacauguac cuagaccgca acgacgcagg cgaagcaaua uccuucccaa      180

ccacgcuggg uaugaacaaa ugcuacauac aaauaaugga ccucggacac augugcgacg      240

caacaauguc guacgaaugc ccgaugcuag acgaaggagu agagccagac gacgucgacu      300

gcuggugcaa cacaaccucc acgugggucg uguacggcac augccaccac aaaaagggag      360

aggcccgaag aagccgacga gccguaaccc uccccucaca cucgacccgc aaacuucaga      420

cacgcagcca aacauggcua gaaagcagag aauacaccaa gcaccugauc aggguagaaa      480

auuggauauu ccgcaacccc ggauuugccc uagcagccgc agccaucgca uggcuccugg      540

gauccagcac aucacagaaa guaauauacc uuguaaugau ccuacuaauc gcaccagccu      600

acucccuccg gugcauaggc gucagcaacc gcgacuucgu cgaaggcaug ucaggcggga      660

ccuggguaga cguaguacuc gaacaugggg gaugcgucac cguaauggca caggacaaac      720

caaccgucga caucgagcug guaaccacaa caguaagcaa cauggcagaa gugcgcucau      780

acugcuacga agcauccauc agcgacaugg ccagcgacuc ccgcugccca acacaaggcg      840

aagccuaccu agacaaacaa agcgacaccc aauacgucug caagagaacc cucguagacc      900

gaggcugggg uaacggcugc ggacucuucg gcaaaggcuc acucgugacu ugcgccaagu      960

ucgcaugcag caagaaaaug acaggcaaaa gcauacaacc agaaaaccua gaauacagaa     1020

ucaugcucag cguucacgga ucccaacacu ccgggaugau cgucaacgac acaggccacg     1080

aaacagacga aaaccgcgca aaagucgaaa ucacaccaaa cagcccacgc gcagaagcaa     1140

cacuuggcgg auucggcagc cucgggcuag acugcgaacc ccgaacgggc cuggacuuca     1200

gcgaccuaua cuaccuaacc augaacaaca aacacuggcu aguccacaaa gaaugguucc     1260

acgacauccc ccucccaugg cacgccggag ccgacacagg aacaccccac uggaacaaua     1320

aagaagcccu aguagaguuc aaagacgccc acgccaaacg ccaaacagua guaguucuag     1380

ggagccaaga aggagcaguc cacacagcgc ucgcaggcgc ccuggaggca gaaauggacg     1440

gagccaaagg acggcucucc agcggacacc uuaagugccg gcuaaaaaug gacaaacuca     1500

gacucaaagg cguaucuuac aguuuaugua ccgccgccuu uacauucacc aagauccccg     1560

cugaaacacu ucacgggacc gucacagucg aaguccaaua cgcggguacc gacggaccau     1620

gcaagguccc cgcgcaaaug gcaguagaca ugcagacauu aacacccgua ggccggcuua     1680

ucacagcaaa cccaguaauc acagaaucaa cagaaaacuc aaaaaugaug cucgagcuag     1740

acccgcccuu cggggacuca uacaucguca ucggagucgg agagaaaaag aucacacacc     1800

acuggcaccg cagcggcagc acgaucggaa aagcguucga agccacugug cgcggagcga     1860

aaagaauggc aguacuggga gacacugcgu gggacuucgg cucagucgga ggggcacuaa     1920

acagccuggg caaaggaaua caccaaauau ucggcgcggc auucaaaucc cucuucggag     1980

gaaugaguug guucucacaa auccugaucg gcacccuacu cauguggcug ggucucaaca     2040

ccaaaaacgg gucaauaagc cucaugugcc ucgcacuagg cggaguacua aucuuccuca     2100

guacagcagu aagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7348
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 2 opt

<400>  7348
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucgggau aguaggccua cuacucacca ccgcuauggc agcagaagua acccgaaggg      120

gcagcgcaua uuacauguac cuagacagaa acgacgccgg cgaagcgauc aguuucccga      180

caacgcuggg aaugaacaaa ugcuacauac aaaucaugga ccucggacac augugugacg      240

ccaccauguc auacgaaugc ccgaugcucg acgagggagu cgaacccgau gauguagacu      300

gcuggugcaa cacaacaagc acuugggucg ucuacgggac cugccaccac aagaaaggcg      360

aagcacgucg cagccgcaga gcuguaaccc ucccaucgca cagcacacgc aaacuccaaa      420

cccgcuccca aacguggcug gaaucccgcg aguacaccaa acacuuaauc agaaucgaaa      480

auuggaucuu ccgcaacccc ggcuucgcac ucgcggcagc cgccauagcg uggcuccuag      540

gcucauccac gagucaaaaa guaauauacc ucgucaugau ccuacuaaua gcccccgccu      600

acuccauacg cugcauaggc gucagcaacc gagacuucgu agaaggaaug ucuggaggca      660

ccugggucga cguaguacug gaacacggcg gcugcgucac ggugauggca caagacaaac      720

cgaccguaga caucgaacuc gucaccacca caguaagcaa cauggcugag guccgcucgu      780

acugcuacga ggcaagcaua agcgacaugg caucagacuc caggugcccc acccaaggag      840

aagccuaccu cgacaaacaa agcgacaccc aauauguaug caaaaggacc cuagucgacc      900

gcgggugggg aaacggcugc ggacuauucg gcaaaggaag ccucguuacc ugcgcaaaau      960

ucgcaugcuc caagaaaaug accggcaaau cuauacaacc agaaaaccuc gaauacagaa     1020

uaaugcuauc aguccacggc ucacaacaca gcggcaugau cgucaaugac accggccacg     1080

aaaccgacga aaacagagca aaaguagaaa ucacccccaa cagcccacga gccgaggcca     1140

cccucggcgg cuucggaucg cuggggcuag acugcgaacc cagaacaggc cucgacuucu     1200

cugaccuaua cuaccuaacg augaacaaca aacacuggcu cguccacaaa gaaugguucc     1260

acgacauccc acuaccaugg cacgcaggcg cagacacagg aacaccccac uggaauaaua     1320

aagaggcacu ugucgaguuc aaagacgcac acgccaaaag acaaaccguc guaguccucg     1380

gcagccaaga aggugcugua cacaccgcac ucgccggcgc ccucgaagca gaaauggacg     1440

gcgcaaaagg ccgccuauca ucaggccacc uaaaaugccg ccucaaaaug gacaaacucc     1500

gacuaaaagg cgucagcuac ucgcucugca ccgcggccuu caccuucacc aaaauccccg     1560

cagaaacccu acacggaacc gugaccgucg aaguccaaua cgcaggaacc gacggaccau     1620

gcaaaguccc agcacaaaug gccgucgaca ugcagacacu aacaccagua ggcagacuca     1680

uaacugccaa cccaguaauc acagaaucca ccgaaaacag uaaaaugaug cucgaacuag     1740

acccgccauu cggcgacagc uacaucguca ucggcgucgg ggaaaagaaa aucacacauc     1800

acuggcaccg cucaggaucc acaaucggca aagccuucga agccacaguc cgaggggcua     1860

aaagaauggc aguccucggc gacaccgcau gggacuucgg cagcguaggc ggcgcccuaa     1920

acucacucgg aaaaggcauu caccaaauau ucggcgcagc auucaaauca cuuuucggag     1980

gaaugagcug guucucacaa auacuaaucg gaacacuacu aauguggcuc ggacucaaca     2040

ccaaaaacgg guccauaagc cugauguguc uagcccuagg aggaguacuc aucuuccuaa     2100

gcaccgcagu cuccgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7349
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 2 opt

<400>  7349
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cagugggaau aguaggacuc cuacuaacga ccgcaauggc cgcagaaguu acccgaagag      120

gcagcgcaua cuauauguac cucgaccgca acgacgccgg cgaagccauc ucauucccaa      180

ccacacucgg gaugaacaaa ugcuacaucc aaauaaugga ccuaggacac augugcgacg      240

cgaccauguc auacgagugu cccaugcuug acgaaggcgu agaaccggac gacguagacu      300

gcuggugcaa caccacgagc accugggugg uauacggcac cugccaccac aaaaaaggcg      360

aggcgagaag gagcagacga gccgucaccc uucccagcca uucaacccga aaacuacaaa      420

caagaaguca aacguggcua gaaucacgcg aauacacaaa acaccugauc cgcguagaaa      480

acuggauccu aaggaaccca ggauucgcac uggccgccgc ggccaucgcc uggcuccuag      540

ggagcagcac aucccaaaaa guaaucuacc uagucaugau ccuccugaua gcgcccgcgu      600

acagcaucag auguaucggc gucuccaacc gagacuucgu agaaggaaug ucugggggua      660

caugggucga cgucguccua gaacacggcg gcugcgucac aguaauggca caagacaaac      720

caacgguaga cauagaacuc gugaccacca ccgucucaaa cauggcagaa guacgaucgu      780

acugcuacga ggcguccaua ucagacaugg ccagcgacag ccgcugccca acccagggag      840

aggcauaccu cgacaaacaa uccgacaccc aauacgugug caaaaggaca cuagucgacc      900

gggggugggg gaacggcugc ggccucuuug gaaaaggcag ccucgucaca ugcgccaaau      960

ucgccugcuc caaaaaaaug acagggaaaa gcauccaacc agaaaaccua gaauaccgaa     1020

ucaugcucuc cgugcacggc ucacaacacu ccgggaugau aguaaacgac acaggacacg     1080

agaccgacga aaaccgagcc aaaguugaaa uaacccccaa cagccccaga gcugaagcca     1140

cacuuggagg auucggaucc cuaggccucg acugcgaacc acgcacgggc cucgacuuua     1200

gcgaccuaua cuaccuaacc augaacaaca aacacuggcu gguccacaaa gaaugguucc     1260

acgacauccc gcugccaugg cacgccggcg ccgacacggg aaccccccac uggaacaaca     1320

aagaggcacu cgucgaauuu aaagacgcac acgccaaacg acaaaccguc guuguacuag     1380

gcucacaaga gggcgcgguc cauacagcac uagcaggcgc ccuggaggcc gaaauggacg     1440

gagccaaagg ccgacucucc agcggacauc uaaaaugccg acugaagaug gauaaacuac     1500

gacucaaagg agucucauac ucacuaugua ccgcagcauu uacauucaca aaaauacccg     1560

cagaaacacu acacggaacc guaaccgucg aaguccagua cgccggcacg gacggccccu     1620

gcaaaguccc cgcccaaaug gccgucgaca ugcaaacacu gaccccaguc ggccgccuca     1680

uaaccgcaaa cccagucauc acugaaucca ccgagaacag caaaaugaug cucgagcucg     1740

acccccccuu cggcgacagc uacaucguca ucggcaucgg ggaaaagaaa auaacacacc     1800

acuggcaccg auccggcagc accaucggca aagccuucga ggcaacaguc cgcggagcaa     1860

aacgcauggc aguccucgga gacaccgccu gggacuucgg cagcgucgga ggagcccuca     1920

acucucuggg caaagggaua caccaaaucu ucggagcagc auucaaaagc cuauucggcg     1980

gaaugagcug guucucccaa auacucauag gaacccuccu cauguggcuc ggacuaaaca     2040

ccaaaaacgg auccauaagc cuaaugugcc uagcucucgg aggcgugcua auauucuuaa     2100

guaccgccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7350
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 2 opt

<400>  7350
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguaggaau cgucggccuc cugcuaacca ccgccauggc agccgaaguc acacgccgcg      120

gcucagcaua cuacauguau cucgacagaa augacgccgg ugaagccaua ucauucccca      180

ccacacuggg caugaauaag ugcuacaucc aaaucaugga ccugggccac augugcgacg      240

ccacaauguc cuacgaaugu cccaugcucg acgaaggcgu cgagcccgac gacguagacu      300

gcuggugcaa caccacuagc acgugggucg ucuacggaac cugccaccac aagaaaggcg      360

aagcccgccg aucaagaagg gcagucaccc uucccucaca cagcacccga aaauuacaaa      420

caagaagcca aacauggcuc gaaagccgcg aauacacaaa acaccuuauc cgcguagaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuacuug      540

gcucauccac cagccaaaaa gucaucuacc uagucaugau ccuacucauc gcacccgccu      600

acuccauccg augcaucggc guauccaacc gcgacuucgu cgaaggaaug aguggcggca      660

ccuggguaga cguuguccuc gaacacggcg gaugcguaac cgucauggcc caagacaaac      720

cgaccgucga cauagaacuc gucucaacca ccguaagcaa cauggcagaa guaagaucau      780

acugcuacga agcaaccaua agcgacauug cgucggacuc ccgcugccca acucagggcg      840

aagccuaccu agacaaacaa ucagacaccc aauacgugug caaacgaacc cucguggaua      900

gaggcugggg caaugggugc gggcuuuucg ggaaaggcuc ccuggucaca ugcgccaaau      960

ucgccuguag caagaaaaug acaggcaaau ccauccaacc ggaaaaccuc gaauaccgca     1020

ucaugcuauc aguacacggc agccaacaca gcggcaugau uguaaacgac acaggacacg     1080

aaaccgauga aaaccgagcc aaagucgaaa uuacccccaa cucaccccgc gccgaggcaa     1140

cccuuggagg cuucggcucc cucggacugg acugcgaacc aagaacaggc cucgacuuuu     1200

ccgaccucua cuaccuaacc augaacaaca aacacuggcu cguacacaag gaaugguucc     1260

acgacauccc ccuucccugg cacgcgggag cggacaccgg caccccacac uggaacaaca     1320

aagaagcgcu ggucgaguuc aaagacgccc augccaaacg ccaaacagcc gucgugcucg     1380

ggagccaaga aggagcaguu cacaccgccc uagccggcgc ccuggaagca gaaauggacg     1440

gcgccaaagg gcgacucucc uccggccacc ucaaaugccg acuaaagaug gacaaauuac     1500

gccuaaaagg agucuccuau agccuaugca cagccgcauu cacauucaca aagauacccg     1560

cagaaacccu ccacggcacc guaaccgucg aaguacaaua cgcaggaacg gacggaccau     1620

gcaaaguacc cgcccaaaug gcagucgaca ugcaaacccu aacccccguc ggacgacuca     1680

uaaccgccaa cccagucauc acagaaagca ccgaaaacuc aaaaaugaug cucgaacucg     1740

acccacccuu cggcgacuca uacaucguca ucggcgucgg agaaaagaaa auaacccacc     1800

acuggcaccg cuccggcagc acgaucggga aagcauucga agccacaguc cgaggagcca     1860

agcgaauggc aguccuaggc gauacagccu gggacuucgg cucaguaggu ggcgcccuaa     1920

acucacucgg aaagggaauc caccaaauau uuggcgccgc auucaaaucc cuauucggcg     1980

gcauguccug guucagccag auauuaauag gaacacuacu cauguggcua gggcucaaua     2040

ccaaaaacgg cuccaucucg cuaaugugcc uagcccucgg uggcguacuc aucuuccuca     2100

gcaccgcagu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7351
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 2 opt

<400>  7351
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguuggaau cguaggacua cuacugacca ccgccauggc ugccgaaguc acacgccgag      120

gcucagccua cuacauguac cucgaccgaa acgacgccgg ugaagcaaua ucauucccaa      180

ccacacucgg caugaacaaa ugcuacaucc aaaucaugga ccucggacac augugcgacg      240

ccaccaugag cuacgaaugu cccaugcuag augagggcgu cgaaccagac gaugucgacu      300

gcuggugcaa caccaccucc accugggucg uguacgguac cugccaccac aagaaaggcg      360

aagcccgacg aucacgacgc gcagucacac ucccaagcca cagcaccagg aaacuccaaa      420

cacgcagcca gacauggcuc gaaagccgcg aauauacaaa acaccuaaua agaguagaaa      480

acuggaucuu ccgaaaccca ggcuucgccc ucgcagccgc cgcgaucgca uggcuacucg      540

ggagcucaac cucccaaaaa gucaucuacc uaguuaugau acuacuaaua gcacccgcuu      600

acuccauccg augcauaggc gucucaaacc gcgacuucgu cgaaggaaug agcggaggca      660

ccuggguaga cguaguccuc gagcacggag guuguguaac cgucauggcu caagacaaac      720

caacggucga cauagagcua guaacaacaa ccgucucaaa cauggcagaa guccggagcu      780

acugcuacga agccagcauc uccgacaugg ccucugauuc ccgcugccca acacaaggcg      840

aggcuuaccu agacaaacag ucagacaccc aguauguuug caaacggaca cucgucgacc      900

gagggugggg caacggaugc ggccucuucg gcaaaggcuc ccucguaaca ugcgcaaaau      960

ucgcuuguuc aaagaaaaug acaggcaaau caauccaacc ggagaaccuc gaauaccgca     1020

ucaugcucag cguccacggg agccaacacu ccggaaugau agucaacgac accggacacg     1080

aaaccgacga aaaccgagca aaaguggaaa ucacgccuaa cagcccccgg gccgaagcaa     1140

cccuaggagg cuucgggucc cuggggcuug acugcgaacc ucguaccggc cuagacuuca     1200

gcgaccucua cuaccuaacc augaacaaca aacauuggcu aguacacaaa gagugguucc     1260

acgacauccc cuuacccugg cacgccggag cagacaccgg cacaccccac uggaacaaca     1320

aagaagcccu cguugaguuc aaagacgcac acgcaaaacg ccaaaccgua gugguucuag     1380

gaucccaaga aggagccguc cacaccgccc ucgccggagc acucgaagcc gaaauggacg     1440

gcgcaaaagg caggcucagc agcggacacc ucaaaugccg acuaaaaaug gacaaacucc     1500

gacucaaagg cguguccuac ucccugugca cagccgccuu caccuucacc aaaauccccg     1560

ccgaaacacu ccacggcacg guaacaguag aacuacaaua cgccgggaca gauggaccau     1620

gcaaaguccc cgcacaaaug gcaguagaua ugcaaacacu aacccccgua ggccgccuua     1680

ucaccgcaaa ccccgucauc accgaaucca cagaaaacuc caaaaugaug cuugaacucg     1740

acccaccauu cggcgauuca uacaucguca uaggcgucgg agaaaagaaa aucacgcacc     1800

acuggcacag gagcggcucc accauaggca aagcguuuga agccaccgug cgcggcgcca     1860

aacgaauggc aguccucgga gacacagccu gggacuucgg cuccguuggc ggggcacuaa     1920

acucacucgg caaaggcauc caccaaaucu uuggagccgc guucaaauca cucuucggug     1980

gcaugucuug guucucucag auccuaaucg gcacucuucu cauguggcua ggacucaaua     2040

ccaaaaacgg cucgaucagc cucaugugcc ucgcacucgg cggcguacuc auuuuccuca     2100

gcacagccgu cucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7352
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 2 opt

<400>  7352
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

ccguaggaau cgucggacuc cugcucacaa ccgccauggc cgccgaaguc acaagacgag      120

gcuccgcaua cuacauguac cucgaccgca acgacgccgg cgaggcaauc agcuucccaa      180

ccacccucgg caugaacaaa ugcuacauac aaauaaugga ucuaggacac augugcgacg      240

ccacgaugag cuacgaaugc cccaugcugg acgaaggagu ggagccagac gacgucgacu      300

gcuggugcaa caccacaagc acauggguug uauacggaac cugccaccac aagaaaggcg      360

aggcccgccg cagcagacga gcaguaacuc uaccauccca cuccacacgc aaacuccaaa      420

cucgauccca gaccuggcuc gaaagccgag aauacacaaa acaucucaua cgcgucgaaa      480

acuggauauu cagaaaccca ggguucgcac ucgccgccgc cgccaucgcc uggcuccuag      540

gcagcagcac aucccaaaaa gucaucuacc uaguaaugau cuuauuaaua gcccccgcgu      600

acuccauacg gugcaucgga guaucaaacc gagacuucgu agaaggaaug agcgggggca      660

cgugggucga cguuguccuc gaacacggcg gcugcguaac cguaauggcc caagacaaac      720

caacaguaga cauagaacuc gucaccacaa ccgucagcaa cauggccgaa gugcgcucau      780

acugcuacga agccuccauu uccgacaugg ccucugacag uaggugccca acgcagggag      840

aagcauaccu agacaaacaa agcgacaccc aauacgucug caagcgcacu cuagucgacc      900

gaggaugggg aaacgggugc ggucuauucg gaaaaggcag ucuuguaacc ugcgcaaaau      960

ucgcuugcuc caagaaaaug acaggcaaaa gcauacaacc cgaaaaccuc gaauaccgca     1020

ucaugcucuc cguccacggc ucacaacacu cagggaugau cgucaaugac acaggccaug     1080

aaaccgacga aaaccgggcc aaagucgaaa ucacccccaa cagccccaga gccgaagcaa     1140

cccuaggagg cuucggcagc cuaggccucg acugcgaacc ucgcaccggu cuggacuucu     1200

ccgaucucua cuaccucacc augaacaaca aacauuggcu cguccacaaa gaaugguucc     1260

acgacauccc ccucccaugg cacgccggag ccgacacagg aacaccacau uggaacaaca     1320

aagaagcacu ggucgaguuc aaagaugccc augccaagag acagaccguc gugguccucg     1380

gcucacaaga aggcgccgua cacaccgcac ucgccggagc gcucgaagcc gaaauggacg     1440

gagccaaagg uaggcuaagc uccggacacc ugaaaugcag acuuaaaaug gacaaguuac     1500

gccucaaagg cgucucauac ucccuuugca cagccgcauu cacuuucaca aaaauccccg     1560

ccgaaaccuu acacgggaca guaacugugg aagugcaaua cgccggcacc gacggaccgu     1620

gcaagguccc cgcgcaaaug gcagucgaca ugcaaacacu aacacccgua ggccgccuga     1680

ucacagcaaa cccuguuauc acagaaucaa uagaaaacuc caaaaugaug cuugaacucg     1740

acccaccguu cggagacagc uacaucguaa uaggcgucgg cgaaaagaaa auuacccacc     1800

acuggcaccg cucaggcagc acaaucggaa aagccuucga agccacaguc cggggcgcca     1860

aacgaauggc cguccucggc gacaccgcau gggacuucgg aucaguagga ggcgcacuca     1920

acagucuugg gaaaggaauc caccaaauau ucggagccgc cuucaagagc cucuucggag     1980

gcauguccug guuuagccaa auacuaauag gcacacuccu aauguggcua ggccucaaca     2040

ccaagaacgg cagcaucagc cucaugugcc ucgcccucgg aggaguacua auauuccuau     2100

ccaccgccgu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7353
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 2 opt

<400>  7353
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guguaggaau cgugggucuc cuccugacaa ccgcaauggc cgcagaagua acacggcggg      120

gguccgcaua cuacauguac cucgaccgga acgacgcagg cgaggccauc ucuuucccca      180

ccacccuagg caugaacaaa ugcuacaucc aaauaaugga ccuaggacac augugcgacg      240

caaccauguc cuacgaaugc cccaugcuag augaaggagu cgaaccagac gacguggacu      300

gcuggugcaa caccaccagu accugggucg ucuacggaac augccaccac aagaaagggg      360

aggcucgcag aagccgccga gcugucacac ucccaagcca cagcaccaga aaacuccaaa      420

cacgauccca aacauggcua gaaucgcgcg aguacaccaa acaccucauc cgcguggaaa      480

acuggaucuu ccgaaaccca ggcuucgccc ucgcagcggc agcaauagca uggcuguuag      540

gauccuccac aagccaaaaa guaauauacc ugguaaugau ccuacucauc gcacccgcau      600

acuccauccg augcauagga guauccaacc gggacuucgu cgaaggaaug agcggaggaa      660

ccugggucga cgugguccua gaacacggcg gaugcgucac cgucauggca caagacaaac      720

ccacagugga caucgagcua guaaccacca ccgugucaaa cauggcugaa guuagauccu      780

acugcuacga agcaagcauc agcgacaugg cgagugacag cagaugcccc acacaaggcg      840

aagcauaccu cgacaaacaa uccgauaccc aauacguaug caaacgcaca cucguagacc      900

gcgguugggg caacggcugc ggauuauucg ggaaagggag ccucguuacc ugcgcaaaau      960

ucgccugcag caaaaagaug acaggcaaaa gcauccaacc cgagaaucua gaauacagaa     1020

uaaugcucuc cguacacgga ucccaacacu cagggaugau aguuaaugac acaggccacg     1080

aaacagacga aaaccgagcc aaaguugaaa uaaccccgaa cucaccaaga gccgaagcca     1140

cccuaggagg cuucggaucu cucggacucg acugcgaacc ccgcacagga cuagacuucu     1200

ccgaccuaua cuaucucacu augaacaaca agcacuggcu cguacauaaa gaaugguucc     1260

acgacauccc acuacccugg cacgcgggcg cagacacagg gacgccccac uggaacaaua     1320

aagaagcgcu cguagaguuc aaggaugcuc acgccaagcg ccaaacaguc guaguacuag     1380

gcucccaaga aggcgccguc cacacagccc uagcaggagc ccuggaagcc gaaauggacg     1440

gaguaaaggg cagacucucu agcgggcacc uuaaaugccg acugaaaaug gacaaacuca     1500

gguuaaaggg cguauccuau ucacugugca ccgcggcauu cacauucacc aaaaucccgg     1560

ccgaaacacu ccacggcaca guaacggucg aaguccaaua cgccggcacg gacggacccu     1620

gcaaaguacc cgcccaaaug gcagucgaca ugcaaacccu cacacccguc ggaaggcuaa     1680

uaacagccaa ccccgucauc accgaaagca ccgaaaacag caaaaugaug cucgaacuag     1740

acccacccuu cggcgacuca uacauaguga ucggcgucgg cgaaaagaaa aucacgcacc     1800

acuggcacag aucaggaucg acaauaggaa aagccuucga agcgacaguc cgcggagcaa     1860

aacgaauggc cguauuaggg gacacagcau gggacuucgg cuccgucggc ggcgcacuca     1920

acucccucgg uaaaggaaua caccaaauau ucggugcagc uuucaaauca cuauucggcg     1980

gcauguccug guucagccaa auucucaucg gaacacuccu cauguggcuc ggccuuaaca     2040

cuaaaaaugg cucaaucucg uucaugugcc ucgcccuagg cggaguccug aucuuccucu     2100

ccaccgcggu auccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7354
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 2 opt

<400>  7354
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggaau agucggacua cuacucacaa cggcaauggc agccgaaguc acccgaagag      120

ggagcgccua uuacauguac cuagacagaa acgacgcagg agaagcaauc ucauucccca      180

cgacacucgg gaugaacaaa ugcuacauac aaauaaugga ccuaggacau augugcgacg      240

cgacaauguc auacgaaugc ccaaugcucg acgaaggagu ggaacccgac gacguagacu      300

gcuggugcaa caccacaucc accugggugg uauauggcac augccaccac aagaaaggcg      360

aagcacgccg aagccgccga gcagucacac ucccaagcca cagcacccgc aaacuccaga      420

caagaagcca aacauggcua gaaucccgag aauacacuaa acaccuaaua cggguagaaa      480

acuggauauu cagaaaccca ggauucgcac uugcagccgc agccaucgcu uggcuccuag      540

gaagcuccac gucccaaaaa gugaucuauc ucgucaugau acuacugauc gcccccgccu      600

acagcauacg cugcauaggc guaagcaacc gagauuucgu ggaaggaaug agcggcggca      660

ccuggguaga cgucguucua gaacacggag gcugcguaac cguaauggcc caagacaaac      720

ccaccgugga caucgaacug guuacaacca ccguauccaa cauggccgag guuagaagcu      780

acugcuacga agcguccauc ucagacaugg ccucagacuc acgcugcccc acacaaggag      840

aagcauaccu cgacaaacaa ucagauacgc aguacgucug caaaagaaca cugguggacc      900

gcggcugggg aaacggaugc ggccuguucg guaaaggcag ccucgucaca ugcgcaaaau      960

ucgcgugcuc gaaaaagaug acaggcaaaa gcauacaacc cgaaaaccua gaauaccgaa     1020

ucaugcuauc cguccacggc ucacaacacu cuggaaugau cgucaacgac acgggccaug     1080

aaacggacga aaacagagcc aaagucgaga uaacacccaa cagccccagg gccgaagcga     1140

cacucggagg cuucgguagc cugggacuag acugcgaacc gcgcacaggc cuggacuuca     1200

gcgaccuuua cuaccugacc augaauaaca aacacuggcu cguccacaag gaaugguucc     1260

acgacauccc ccugccaugg caugccggcg cagacaccgg caccccacac uggaacaaca     1320

aagaagcccu aguagaguuc aaagacgcac acgcaaaacg ccagacagua guaguccugg     1380

gcucacagga aggcgcagug cacaccgcac uggcaggcgc gcuggaagcc gagauggacg     1440

gcgccaaagg uaaacuaucu ucgggccacc ucaaaugccg ucuaaaaaug gacaaacuaa     1500

gacuaaaagg ggucuccuac agccucugca ccgcagccuu caccuuuaca aaaauaccag     1560

cagaaacacu ccacgggaca guaaccgucg aagugcagua cgccggcaca gacggccccu     1620

gcaagguccc cgcacagaug gcagucgaca ugcagacacu caccccagua ggacgccuca     1680

uaaccgcaaa ccccgucauc accgaaagca ccgagaacag uaaaaugaug cucgaauugg     1740

acccaccauu cggagacagc uacaucguaa uaggaguugg cgaaaagaaa aucacacacc     1800

auuggcacag aagcgggagc accauaggca aagcauucga agccacgguc cgaggcgcaa     1860

aacggauggc agugcuaggc gacaccgcau gggacuuugg aagcgugggc ggagcacuga     1920

acagucuagg aaaaggcaua caccagauau ucggagcagc guucaaaagc cuguuuggcg     1980

gaaugucaug guucucgcaa auccucauag gcacccuacu uauguggcua gggcucaaca     2040

ccaaaaaugg aagcaucagc cuaaugugcc uagcacuagg gggcguccua auauuccuca     2100

gcaccgccgu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7355
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 2 opt

<400>  7355
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gcgucggaau cgucggacuc cuccuaacca ccgcaauggc ggccgaaguc acccgccgag      120

gcagcgcaua cuacauguac cuagaccgaa aagacgccgg agaagcgauc agcuucccca      180

ccacacuagg aaugaacaaa uguuacauac aaaucaugga ucugggacac augugcgacg      240

ccaccaugag cuacgaaugc cccaugcucg acgaaggcgu agaaccggac gacguagacu      300

gcuggugcaa caccaccagc accugggucg uauacggaac cugccaccac aagaaaggag      360

aagcacggcg aagccgacgc gccgucacac uccccucaca cagcacccgc aaacuccaaa      420

cccgauccca aaccuggcuc gagagcagag aauacacgaa acacuuaaua cgaguagaga      480

acuggauauu ccgcaacccc ggauucgcac uagcagcagc cgccaucgcc uggcuacucg      540

gcucauccac aucacaaaaa gucaucuacc uggucaugau acuacuaaua gcacccgccu      600

acagcauacg cugcauagga guaagcaacc gcgacuucgu cgaaggaaug uccggcggca      660

ccuggguaga cguaguccug gagcacggcg gcugugucac cguaauggca caagacaaac      720

ccaccguaga caucgagcua guaacaacca ccgucaguaa cauggcagaa guacgauccu      780

acugcuacga agcaagcauc uccgacaugg cgucagacuc ccgguguccc acgcaaggag      840

aagcauaccu cgacaagcaa agcgacacac aauacgucug caaacgaacc cucgucgacc      900

gaggaugggg caacgguugc ggccuguucg gaaaaggauc ccucgugacc ugcgcaaaau      960

ucgcaugcag caagaaaaug accggcaaga gcauccaacc cgaaaaccua gaauaccgca     1020

ucaugcuguc aguccacggc agccaacaca gcgggaugau agucaacgac acuggccacg     1080

aaaccgacga aaacagagca aaaguggaga uaaccccaaa cagcccccga gccgaggcaa     1140

cacucggagg auucgggagc cucggccucg acugcgaacc caggaccgga cucgacuuca     1200

gcgaccucua cuaccugacc augaacaaca aacacuggcu cguccacaaa gaaugguucc     1260

acgacauacc ucugccuugg cacgcaggcg ccgacacggg aacuccacac uggaacaaca     1320

aagaagcccu cguagaguuc aaggacgccc acgcgaaaag gcaaacggua guuguacuag     1380

gcagucaaga aggcgcagua cacaccgccc uagccggcgc ccuagaagcc gaaauggacg     1440

gcgccaaagg ccgucuaucc agcggacacu uaaagugccg acuaaaaaug gacaaacuuc     1500

gccucaaagg ggucucauac agucucugca ccgcugcguu uaccuucacc aaaauccccg     1560

cagaaacacu ccacggaacu gucacagucg aagugcaaua cgccgggaca gacggccccu     1620

gcaaaguacc agcacagaug gccgucgaca ugcagaccuu aacaccuguc ggaagacuca     1680

ucacagccaa ccccgucaua accgaaucca cagaaaacag caaaaugaug uuagaacucg     1740

acccucccuu cggagacuca uacauaguaa ucggcguggg cgaaaagaaa aucacccacc     1800

acuggcacag auccggaucc accaucggaa aagccuucga agccaccguc cgaggcgcca     1860

aacgcauggc cguauuggga gacacagcau gggacuucgg guccguagga ggcgccuuaa     1920

acucgcuagg gaaaggcauc caccaaaucu ucggagccgc cuucaaaagc cucuucggug     1980

gcaugagcug guucucccaa auccucaucg gcacgcuccu aauguggcua ggucugaaca     2040

ccaagaacgg cucaauaucc cuaaugugcc ucgcacuagg cggaguccua auauuccuau     2100

caaccgccgu cucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7356
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 2 opt

<400>  7356
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgugggaau cguaggacug cuacucacaa cagccauggc agcagaaguc acccgaagag      120

gcucugcgua cuacauguac cuagaccgca acgacgccgg ggaagcgaua ucauucccaa      180

caacacucgg caugaacaaa ugcuacaucc aaauaaugga ccuaggccac augugcgacg      240

ccacaaugag cuacgaaugc ccaaugcucg acgaaggagu ggagccagau gauguggacu      300

gcuggugcaa cacgaccuca accuggguag uauacggcac augccaccac aaaaagggcg      360

aggcccgcag aagccgcaga gcagucaccc uacccucaca cucuacaaga aaacuacaaa      420

cacgcuccca aacauggcua gaaagcagag aauacacaaa acaccuaaua cgaguagaga      480

acuggaucuu ccgaaaccca ggcuucgcau uggcagccgc cgcaaucgcu uggcuacuag      540

gguccagcac cagccaaaaa gucaucuacc uaguuaugau acuccucauc gccccagccu      600

acagcauccg augcauuggu guaucaaaca gggacuucgu agaaggaaug agcggcggaa      660

cauggguaga cgugguccuc gaacacggcg gcugcguaac gguaauggcc caagacaaac      720

ccacaguaga uauagagcua gucacaacga caguaagcaa cauggccgaa guaagaagcu      780

acugcuacga agcaagcaua agcgacaugg ccucggacuc caggugcccc acgcagggcg      840

aagcauaccu agacaagcag agugacaccc aauacgucug caagcgcacg cuaguagaca      900

gaggaugggg aaacgguugc ggccuauucg gaaaaggguc acucgugacc ugcgcaaagu      960

ucgccugcag uaaaaagaug acaggcaaaa gcauacaacc cgagaaccug gaauaccgua     1020

ucaugcucag cguacacggc ucacaacaua gcgggaugau agucaacgac acaggccacg     1080

aaacggacga gaacagagca aaagucgaaa uaaccccaaa cucaccccga gcagaagcaa     1140

cccuaggcgg cuucggaucc cugggacuag acugcgaacc cagaacaggc cuugacuuuu     1200

ccgaucugua uuaccugaca augaacaaca agcacuggcu aguacacaaa gaaugguucc     1260

acgacauacc ccuacccugg cacgcaggcg ccgacaccgg cacaccccac uggaacaaca     1320

aagaagcccu cguagaguuc aaagaagccc acgcaaagcg ccaaacggua gucguacugg     1380

ggagccaaga gggagccgua cacacagcgc uagcgggagc acucgaagca gaaauggacg     1440

gggccaaagg gagacuuagc agcggccacc uaaaaugccg acuaaaaaug gacaaacucc     1500

gccuaaaggg aguaucauac ucccugugca cagcagcauu caccuucacc aagauccccg     1560

cagaaacacu ccauggcaca gucacaguag aaguacagua cgccggcacc gacggacccu     1620

gcaaaguacc agcacaaaug gccguagaca ugcaaacacu aaccccagua ggcagacuca     1680

ucacagcuaa uccaguaaua acagaaagca ccgagaacag uaaaaugaug cuggagcucg     1740

acccgcccuu cggcgacucc uacaucguaa uaggaguggg cgagaaaaag auaacacacc     1800

auuggcacag aagcggcagc accauaggca aagcauucga agcgacggug cgcggggcua     1860

aacgcauggc agugcuaggc gacaccgcgu gggacuucgg cucaguagga ggggcacuaa     1920

acucacucgg aaaaggcaua caccagaucu ucggagcagc cuuuaagagc cucuucggag     1980

gcaugagcug guucucgcaa auacucaucg gcacacuucu cauguggcuc gggcuaaaca     2040

ccaaaaacgg aucaaucagc cucaugugcc uagcacuagg agggguccuc aucuuccuaa     2100

gcacggcggu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7357
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 2 opt

<400>  7357
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguuggaau agucggccua cuacuaacca cagcaauggc cgccgaaguc acacgcagag      120

gguccgcuua cuacauguac cuagaccgaa acgacgcggg cgaagcaauc agcuucccca      180

ccacccucgg aaugaacaaa ugcuacaucc aaaucaugga ccuaggacac augugcgacg      240

cgacuaugag uuacgaaugc cccaugcucg acgaaggagu cgaaccggac gacguagacu      300

gcuggugcaa caccacgucc accuggguag uauacggaac cugccaccac aagaaaggcg      360

aagcacgccg aagccgccgc gccgucacgc ugccaucuca cucaacacga aaacuacaaa      420

cacgaaguca gacauggcua gagagccgcg aguacaccaa acaccugaua agaguugaaa      480

auuggauauu cagaaaccca ggcuucgcgc uggcggcagc agccaucgcc uggcuccucg      540

gaucauccac cagccagaaa gucauauacc uaguaaugau ccuccucauc gcaccagcgu      600

acagcauccg cugcaucggc guaagcaacc gcgauuucgu agaaggcaug agcggaggca      660

ccuggguaga cguaguacuc gaacacggag gaugcguaac cgucauggcc caagacaaac      720

ccacagucga caucgaacuc gucaccacca caguaagcaa cauggccgag guccgcagcu      780

acugcuacga ggcaagcauc agcgacaugg caucggacuc ccgaugccca acccaagggg      840

aagcguaccu agauaaacaa agcgacacac aauacgucug caaacgcaca cuuguagacc      900

gaggaugggg uaacggaugc ggacucuuug gcaaaggcag ccucgucaca ugcgccaaau      960

ucgcaugcuc aaagaaaaug acaggaaaau ccauccagcc cgagaaccug gaguaccgca     1020

ucaugcucag uguccacggg agccaacacu caggcaugau cgucaacgac acgggccacg     1080

aaaccgacga aaaccgagcc aaagucgaaa ucacccccaa cucgccaaga gcagaagccg     1140

cccucggcgg cuucggauca cucggacugg acugcgaacc cagaacugga cucgacuucu     1200

cagaccucua cuaccucacc augaacaaca aacacuggcu cguccacaaa gaaugguucc     1260

acgacauacc ccuacccugg cacgccggug cugacacagg cacuccacac uggaacaaca     1320

aagaagcccu aguagaguuc aaagacgcac acgcaaaacg ccaaaccgua gucguacuag     1380

ggagccaaga aggcgccgua cacaccgcac uagcaggcgc acuagaagca gaaauggacg     1440

gcgcaaaagg cagacugucu agcggccacu uaaaaugccg ucucaaaaug gacaaacuga     1500

gacucaaagg aguaagcuac ucccuaugca cagcagcauu cacauucaca aaaauacccg     1560

cagaaacacu acacggcaca guaaccgucg agguacaaua cgcagggaca gacgggcccu     1620

gcaaaguccc agcacaaaug gccguggaua ugcaaacccu cacacccgua ggacgacuaa     1680

uaaccgcaaa cccagucauu acagaaucca ccgaaaacag caaaaugaug cucgaacuag     1740

acccaccauu cggcgacuca uacaucguca uaggagucgg ggaaaagaaa aucacacacc     1800

acuggcaccg cagcgggucg accaucggca aagccuucga agcgacaguu agaggggcaa     1860

aaaggauggc cguccuaggc gacaccgcgu gggacuucgg cuccguaggc ggagcccuaa     1920

acagucucgg caaaggaaua caccaaaucu ucggagcagc guucaaauca cuauuuggcg     1980

gcaugucuug guucucacaa acccuaaucg ggacccuccu cauguggcuc ggccuaaaca     2040

ccaaaaacgg cuccaucagc cucaugugcu uagcccucgg cgggguccua auauuccuuu     2100

ccacagcagu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7358
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 2 opt

<400>  7358
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguaggaau cgucggccuc cuacuuacca ccgcaauggc cgcagaaguc acccgucgag      120

gcucagccua cuacauguac cucgacagaa acgacgccgg cgaagccauc agcuuuccga      180

caacgcuugg caugaacaaa ugcuacauac aaauaaugga ccucggccac augugcgacg      240

caacgauguc auaugaaugc ccaaugcuag acgaaggcgu agaaccagac gacgucgacu      300

gcugguguaa cacaacaucc accugggugg ucuacggaac cugccaccac aagaaaggag      360

aagcucgacg aagccgacgc gccguaacac ugcccagcca uuccacgcgc aaacuccaaa      420

cacgcuccca aaccuggcuu gagagccgag aguacacaaa acaccuaaua cgcguggaaa      480

acuggauauu ccgaaacccc ggcuucgcac ucgccgcagc cgcaauagcg uggcuucuag      540

gaagcuccac cucacagaaa gugauauauc ugguaaugau acugcucaua gcacccgccu      600

acagcauaag augcaucggc gucucaaaca gggacuucgu cgaaggaaug agcggaggga      660

cgugggucga cgucguccua gaacacgggg gcugcguaac agucauggcg caagacaagc      720

ccacgguaga caucgaacug gucaccacca cagucucaaa cauggccgaa guacgcuccu      780

auugcuauga agccucaauc ucagacaugg cguccgacag ccgcugcccc acccaaggcg      840

aagccuaccu cgacaaacaa ucggacaccc aauacgucug caaaagaaca cuuguagaca      900

ggggaugggg caacggaugc ggacucuucg gcaagggcag ccuaguaaca ugcgccaaau      960

ucgccugcuc aaagaaaaug accggaaagu ccauccaacc cgaaaaccua gaauacagaa     1020

ucaugcuauc cguacacggc agccaacacu ccggcaugau cgucaacgac accggccacg     1080

aaacagacga aaaccgagcc aaggucgaaa uaacccccaa cucacccagg gcggaagcaa     1140

cacuuggcgg auucggcagc cucggccuag acugcgaacc acgcaccggc cuagacuuca     1200

gcgaccucua cuaccuaaca augaacaaca aacacuggcu cguacauaag gagugguucc     1260

augacauacc gcuacccugg cacgcaggcg ccgacaccgg aaccccacac uggaacaaca     1320

aagaagcgcu cgucgaguuc aaagacgccc acgccaaacg ccagaccgua gucguccuag     1380

gaucacaaga aggcgcugua cacacagccc ucgccggagc ccucgaagcc gaaauggacg     1440

gagcaaaagg ccgccucagc agcgggcacc uaaaaugccg acuaaaaaug gacaaacucc     1500

gucucaaagg aguaagcuac ucccucugca cagccgcuuu caccuucaca aaaauaccag     1560

ccgaaacccu ccacggcacc gucacaguag aaguccaaua cgcaggggcu gacggacccu     1620

gcaaaguccc cgcccaaaug gccguagaca ugcaaacgcu caccccaguc ggcagacuua     1680

uaaccgcgaa ccccguaauc accgaaagca ccgaaaacuc aaaaaugaug cucgagcuag     1740

acccacccuu cggcgacagc uacaucguca ucggcguggg agaaaagaag aucacacacc     1800

acuggcacag aagcggcucg acaauaggca aagcauucga agccaccgua cguggcgcga     1860

aacgcauggc cgugcucgga gacaccgcuu gggacuucgg gagcguaggg ggagcccuca     1920

acucccucgg caagggaaua caccaaauau ucggcgcugc cuucaagagc cucuucggcg     1980

gaaugaguug guucucccaa auacucaucg gaacucuccu aauguggcuc ggacucaaca     2040

ccaaaaacgg cucgauuucc cucaugugcc uugcacuugg cgguguccuu auauuucucu     2100

ccaccgccgu aucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7359
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 2 opt

<400>  7359
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucggcau uguaggccua uuacuuacaa cagcuauggc cgccgaaguc acaagacggg      120

gauccgccua cuacauguac cuagaccgca acgacgccgg agaagccaua ucauucccca      180

caacccuagg caugaacaag ugcuacauac aaauaaugga ccuuggucac augugcgacg      240

ccacaaugag cuacgaaugu cccaugcucg acgaaggcgu agagcccgac gacgucgacu      300

gcuggugcaa cacaacaucc acguggguag uauacgggac cugccaccac aagaaaggcg      360

aggccagacg gucacgacga gccguaaccc uacccuccca cuccacccga aaacuccaaa      420

cacgcagcca aacuuggcua gaaucacgag aauacaccaa acaccuaauc cgcguagaga      480

acuggauauu cagaaaccca ggauucgcac uagccgccgc cgcaauagcc uggcuccuag      540

gcucaucgac cucacagaaa guaaucuacc ucguaaugau acuucucauc gcacucgccu      600

acagcauccg cugcaucggc guaagcaauc gcgacuucgu cgagggaaug ucaggaggaa      660

caugggucga cgucgugcua gagcacggcg ggugcguaac ggucauggca caagacaaac      720

ccaccgucga caucgaacuc guaacgacca cagucucuaa cauggccgaa guacgaaguu      780

acugcuacga agccuccaua ucggacaugg caagcgacuc ccgcugcccg acccaaggag      840

aagccuaccu ggacaaacag agcgacacac aauacgugug caaacgaacu cuagucgacc      900

gcggaugggg caacggaugc ggccucuucg gaaaaggaag ccuaguaaca ugcgcgaaau      960

ucgcuugcuc caagaaaaug accgggaaau ccauccaacc ugagaaccug gaauaucgaa     1020

uaaugcuauc cguacacggc ucccaacacu ccggcaugau cgucaacgac acuggacaug     1080

aaacagacga aaaccgcgcc aaaguggaaa ucacacccaa cuccccacgc gcggaagcaa     1140

cgcucggagg auucggcuca cuaggccuag acugcgaacc cagaaccgga cuagacuuca     1200

gcgaccucua cuaccucaca augaacaaca aacacuggcu aguccacaaa gaaugguucc     1260

acgacauccc ccuaccaugg cacgcagggg cggacacggg aacaccccac uggaacaaca     1320

aagaagcucu cguagaguuc aaagacgcgc augcgaaacg acaaaccgua guaguccucg     1380

gaucgcaaga aggagcagua cacaccgccc uggccggcgc ccuagaagcc gaaauggacg     1440

gagcaaaagg ccgacucagc agcgggcacc ucaaaugcag acuaaaaaug gacaaacuaa     1500

gacucaaagg cguaagcuac agccuuugca cagcagccuu uacauucaca aaaauucccg     1560

cugaaacacu ccacggcacc guaaccguag aaguccaaua cgcaggcacc gacggaccau     1620

gcaaaguccc agcacagaug gcugucgaca ugcaaacacu cacacccgua ggacgacuaa     1680

uaacugccaa ccccgugauc accgaaagca cagaaaacag caagaugaug cucgaacugg     1740

acccacccuu cggcgacucc uauaucguca uuggagucgg agaaaagaaa aucacccacc     1800

acuggcaccg cagcggcucc acaaucggca aggccuucga agcaaccgua agaggcgcca     1860

aaagaauggc cguacuagga gacacggcau gggacuucgg cagcguaggc ggggcacuca     1920

acagucuagg aaagggcauc caccaaauau ucggcgcagc cuucaaauca cuauucggcg     1980

gcaugagcug guucagucaa auacucauag gcacgcugcu aauguggcuc ggccucaaua     2040

ccaaaaacgg aucgaucucc cucaugugcc ucgcccuugg aggcgugcua aucuuccuau     2100

ccaccgcagu cagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7360
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 2 opt

<400>  7360
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguaggaau agucggccuc cuccugacca cagccauggc cgccgaagug accagacgcg      120

gcucagcaua cuacauguac cucgacagga acgacgccgg cgaagccauc ucauucccga      180

ccacauuagg caugaacaaa ugcuacaucc aaaucaugga ucuaggccac augugcgaug      240

ccaccauguc auacgaaugc cccaugcucg augaaggcgu cgaaccggac gacguagacu      300

gcuggugcaa caccaccucc accugggucg uauacggaac cugccaccac aagaaaggug      360

aagcacgcag aagccgacgc gccgugacac uucccucaca cuccacccga aaacuacaaa      420

cacgcucaca aaccuggcua gaaucccgcg aauacaccaa acaccucaua cgcgucgaaa      480

acuggaucuu ucgaaaccca ggauucgcuc ucgccgcagc cgcaaucgca uggcuucucg      540

gaucuuccac aucccaaaaa gucaucuacu uaguuaugau ccuccucauc gccccagccu      600

acuccauacg augcaucggc guaagcaacc gcgacuucgu cgaaggcaug ucgggaggca      660

cguggguaga cgucguucua gaacacggug gcugcguaac aguaauggcc caagacaagc      720

ccaccgucga cauugaacuc gucaccacca cuguaucaaa cauggcugaa guccgcagcu      780

acugcuacga agcaagcauc uccgacaugg ucuccgacag ccgcugccca acccaaggcg      840

aagcguaccu agacaaacag ucggacaccc aauacgucug caaacgcacc cuagucgaca      900

gagguugggg caacggaugc ggacucuucg gcaagggauc ccuaguaaca ugugccaaau      960

ucgcgugcuc gaagaaaaug accggaaaaa gcauccagcc cgaaaaccuc gaauaccgca     1020

uaaugcucuc cguccacgga agccaacacu ccggcaugau cgugaacgac accggacacg     1080

aaaccgacga aaaccgcgcu aaaguagaaa ucacaccaaa cucaccccgc gcagaagcaa     1140

cccuaggagg cuucggaucc cucggccuag acugugaacc ccggaccggc cuagauuucu     1200

ccgaccucua cuaccuaacc augaacaaca aacacuggcu cguacauaaa gagugguuuc     1260

acgacauccc ccuccccugg cacgcgggcg cagacacagg uacaccccau uggaacaaca     1320

aagaagcccu ugucgaguuc aaagacgcac acgccaaaag acaaaccguc gucgugcuug     1380

gcucccagga aggagccgug cacacagccc uggccggagc gcuagaagca gaaauggacg     1440

gcgccaaagg aagacuaagc uccggacacc uaaaaugccg acuaaaaaug gacaaacuuc     1500

gacuuaaagg agugucauac ucacucugca cggccgcauu uaccuucacc aaaauccccg     1560

cagaaacacu acacggcacc gucaccgucg aaguacaaua cgccggaacc gacggcccau     1620

gcaagguacc agcacaaaug gccgucgaca ugcagacacu uaccccggua ggccgacuga     1680

uaaccgcuaa cccagucauc accgaaucaa ccgaaaauuc gaaaaugaug cuggaacucg     1740

acccacccuu cggagacuca uauaucguca uaggcguagg agaaaagaaa aucacccacc     1800

acuggcaccg cucuggcagc acaauaggaa aagccuucga agccacaguc agaggcgcca     1860

aacgcauggc cgugcucggc gacaccgccu gggacuucgg aagcgucgga ggcgcccuca     1920

acagccuggg caaaggaauc caccaaauau ucggagcagc cuuuaaaucc cuuuucggag     1980

gaaugucaug guucucccaa auucuaauug gcacacuccu aauguggcuc ggacuaaaca     2040

ccaaaaaugg cucaauaagc cugauguguc uagcacuagg aggcguacuc aucuuccuua     2100

gcaccgccgu cagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7361
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 2 opt

<400>  7361
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguaggcau agucggccua cuccugacaa ccgcaauggc agccgaaguc acacgccgcg      120

gaagcgccua cuacauguac cucgaccgaa augacgccgg cgaagccauc agcuucccca      180

ccacgcucgg caugaacaaa ugcuacauac aaaucaugga ccucggccac augugcgacg      240

ccaccauguc guacgaaugc cccaugcuag acgaaggcgu cgaaccagac gacguagacu      300

gcuggugcaa caccacaucg acauggguag uguacggaac augccaccau aagaaaggag      360

aagcaagacg cagcaggcga gcggucaccc uacccagcca cuccacgaga aaacuccaga      420

cacgcuccca aacauggcuc gaaagcagag aauacacuaa acaccucauc cgaguagaaa      480

auuggauauu uagaaaccca ggcuucgcgc uagcggcagc cgcaaucgcc uggcuacucg      540

guaguuccac aucacaaaaa guaaucuacc ucguaaugau ccuccuaauu gcaccagcau      600

acuccauaag augcaucggc aucuccaaca gagacuucgu ggaaggcaug ucagggggaa      660

cauggguaga cguuguacuc gagcacggcg gcugcguaac cguaauggcc caagacaaac      720

caacagugga caucgaacuc guuaccacaa ccgucaguaa cauggccgaa guccguuccu      780

acugcuacga ggccaguaua uccgacaugg ccagcgacag ccgcugccca acccaagggg      840

aagccuaccu cgacaaacaa ucagacaccc aauauguuug caaaagaacc cuagucgacc      900

gaggcugggg aaacggaugc ggacuuuucg gcaaaggcuc gcucgucaca ugcgccaaau      960

ucgcaugcuc caagaaaaug accggcaaaa gcauacaacc agaaaaccuc gaauaccgca     1020

ucaugcucuc cguacacggu agccaacaca gcgggaugau cgucaacgac acaggccacg     1080

aaaccgacga aaaccgcgcg aaagucgaaa uaaccccuaa cucaccccgu gccgaagcaa     1140

cacuuggcgg guucgguauc cugggccucg acugcgaacc cagaaccggc cucgacuucu     1200

ccgaccucua cuaccuuacc augaauaaca aacacuggcu aguccacaaa gaaugguucc     1260

acgacauacc gcuccccugg cacgccggag ccgacacagg cacaccccac uggaacaaca     1320

aagaagcccu gguagaguuc aaggacgcgc acgcaaaacg acagaccgua gucgugcucg     1380

gcagucaaga aggugcugug cacacggcac ucgccggugc acuggaagca gaaauggacg     1440

gagcaaaagg ccgccuaucc agcggccauc ucaaaugccg acucaaaaug gacaaacuac     1500

gucuaaaagg agugucguac agccuaugca cagccgccuu cacauucacg aaaaucccag     1560

ccgaaacacu acacggaacg guaacaguag aaguacaaua ugcaggcacc gacggaccau     1620

gcaaaguacc cgcccaaaug gccguugaca ugcaaacucu aacuccagua ggacgccuga     1680

uuaccgccaa cccagucauc accgaaagca ccgaaaacuc uaaaaugaug cucgaacugg     1740

acccaccauu cggugacagc uacaucguca uaggcguagg agaaaagaaa aucacccacc     1800

acuggcaccg cagcggcucc accauaggca aagcauucga agcaaccguc cggggagcca     1860

aacgcauggc aguccucggc gacaccgccu gggauuucgg guccgucggc ggggcccuca     1920

auucccuggg gaaaggaaua caccaaaucu ucggggccgc cuucaaaagc cuuuucggcg     1980

gaaugucaug guucucucaa auacucaucg gaacccuacu aauguggcuu ggccugaaca     2040

ccaaaaacgg aagcaucuca cucaugugcc ucgcacuugg aggcgugcuc aucuuccuau     2100

ccaccgcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7362
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 2 opt

<400>  7362
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggcau cguaggacuc cuccuaacaa ccgccauggc agcagaaguu acacgccggg      120

gaucagcaua cuacauguac cuagaccgca acgacgccgg ugaagccauc ucguucccca      180

caacacuggg caugaacaaa ugcuacaucc aaaucaugga ccucggacau accugcgacg      240

caacaauguc auaugaaugc cccaugcuag acgaaggcgu cgaacccgac gacguagacu      300

gcuggugcaa caccaccagc acguggguag ucuauggcac cugucaccac aagaaaggcg      360

aagcacgccg uucucgcaga gcagucaccc uacccucaca cagcauccgc aaacuucaaa      420

cccgcuccca aacauggcuc gagucccggg aauauacgaa acaccucaua cgaguagaaa      480

acuggaucuu ccgcaacccc ggcuuugccc uagccgcagc agcaauagcc uggcuauuag      540

gcuccagcac cagccaaaaa guaaucuacc uggucaugau ccuccucauu gccccugcau      600

acucaauacg cugcaucgga gucucaaaca gggauuucgu cgagggaaug ucgggcggaa      660

cgugggucga cgucguccuc gaacacggug gcugcgugac agucauggcc caagacaaac      720

cgacaguuga cauagaacuu gucaccacaa caguaucaaa cauggcggaa guccgcagcu      780

acugcuacga agccucaaua uccgacaugg caagcgacag ccgaugccca acccaaggcg      840

aagcauaccu cgacaaacaa uccgacacgc aauauguaug caaacgcacc cuaguagacc      900

ggggcugggg aaacggcugc ggacucuucg gcaaaggcag ccuagucaca ugcgcuaaau      960

ucgccuguag caagaaaaug acaggcaaaa gcauccaacc cgaaaaucuc gaguaccgaa     1020

ucaugcuaag cguacacggc ucacaacacu caggaaugau agucaacgac acaggacacg     1080

agacagacga aaaccgcgca aaagucgaga uaacgcccaa cuccccacgc gcagaagcaa     1140

cacucggcgg cuucggaagu cucggucuag acugcgaacc ccgaacagga cuagacuucu     1200

cagaccuaua cuaccugacu augaacaaca agcacuggcu cguccacaaa gaaugguuuc     1260

acgacauccc cuuaccaugg cacgcaggcg cugacaccgg cacaccacac uggaacaaca     1320

aagaagcacu cgucgaguuc aaagacgcuc acgccaaacg gcaaaccguc guuguccugg     1380

gcucccaaga aggcgcggua cacaccgccc ucgccggcgc ucucgaggca gaaauggacg     1440

gagcaaaagg acgccucucc aguggacacc ucaaaugcag acuaaaaaug gacaaacuac     1500

gccucaaagg ugucucauac agccucugua cagcagcauu caccuucacc aaaaucccgg     1560

ccgagacacu ccacggaacc guaaccguag aaguccaaua cgcagggaca gacggacccu     1620

gcaaaguccc agcacaaaug gcaguagaca ugcaaacacu aacacccgug ggaagacuca     1680

ucaccgcaaa ccccgucauc accgaaagca ccgaaaacag caaaaugaug cuagaacuag     1740

acccacccuu cggcgacuca uacauaguaa uaggcguggg agaaaagaaa aucacccacc     1800

acuggcaccg cagcggauca accauaggca aagcauucga agccacaguc cgcggugcaa     1860

aacgcauggc cguccucggc gacacagccu gggacuucgg cagcguaggc ggcgcccuaa     1920

acucgcuagg aaaaggcaua caccagaucu ucggggcagc cuucaagagu cucuucggcg     1980

guauguccug guucagccaa auccucaucg ggacccuacu cauguggcuc ggccucaacg     2040

ccaaaaacgg cucgaucuca cucaugugcc uagcacucgg cggcguccuc auauuccugu     2100

caacagccgu aagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7363
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 2 opt

<400>  7363
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cagucgggau agucggccua cuacuaacca cggccauggc agccgaagua acccgacgcg      120

gaucggcaua cuacauguac cuagacagaa acgacgcagg agaagccaua ucuuucccca      180

ccacauuagg caugaacaaa ugcuacaucc aaaucaugga ccuaggacac augugcgacg      240

caacgauguc guaugagugc cccaugcucg acgaaggggu ggaacccgac gacguagacu      300

gcuggugcaa cacaaccuca accugggucg uauacggaac cugccaucau aagaaagggg      360

aagcccaacg cucccgcaga gccguaacac uacccagcca cagcaccaga aaacuacaaa      420

cccgcucaca aaccuggcug gaaucacgcg aguacaccaa acaccuaaua aggguagaaa      480

acuggauauu ccggaaccca ggcuucgccc ucgccgcagc agcaauagca uggcuacucg      540

gauccucaac aucccagaaa gugaucuacc ucguuaugau acuccuuauc gcccccgccu      600

acagcauccg augcauaggc gucagcaacc gagacuucgu agaaggaaug agcggcggca      660

ccuggguaga cgucguacuc gagcacggcg guugugucac cgucauggcc caggacaaac      720

caacaguaga cauugaacuc gugacaacaa cggucuccaa cauggcagaa guacgcagcu      780

acugcuacga agccagcauc agcgacaugg ccuccgacag ccgcugcccc acacaaggag      840

aagcguaccu ggacaaacaa ucagacaccc aauacgucug caaacgcacc cuagucgacc      900

gaggaugggg caacggaugc ggccucuucg gcaaaggauc gcucguaaca ugcgccaaau      960

ucgccugcag caagaaaaug accggaaaau caauccaacc cgaaaacuua gaauaccgca     1020

uaaugcuauc aguccacggc ucacaacaca gcggcaugau cguaaacgac accggccacg     1080

aaaccgauga gaaccgcgca aaggucgaaa uaacccccaa cagcccccgc gccgaagcaa     1140

cccuaggagg auuuggaagc cucggccuag acugcgaacc acgcaccggc cucgacuucu     1200

ccgaccuaua cuaccucacc augaacaaca agcacuggcu gguccacaaa gaaugguucc     1260

acgacauccc ccuccccugg cacgccggag ccgacacggg aacaccccac uggaauaaca     1320

aagaggcccu aguagaguuc aaagacgcac acgcgaaacg gcaaacagua guuguacucg     1380

gaucacaaga aggggccgua cacacagccc uagcaggagc acucgaagcg gaaauggacg     1440

gagccaaagg cagacucagc agcgggcauc uaaaaugccg acucaaaaug gacaaacucc     1500

gauugaaagg cgucagcuac agccucugua ccgcagcauu uacauucacc aagauacccg     1560

ccgaaacacu acacggaaca guaaccguag aaguccaaua cgccgggaca gacgggcccu     1620

gcaagguacc cgcacagaug gcaguggaca ugcaaaccuu aacaccggua ggacgacuga     1680

ucacagcgaa cccagucauc acggaaucaa ccgaaaacuc caaaaugaug cuggaacuag     1740

acccgccuuu cggcgauagc uacaucguaa uuggaguggg cgaaaagaaa aucacacacc     1800

acuggcaccg cuccggcuca acaaucggaa aagccuucga agccaccguc cgcggcgcca     1860

aaagaauggc aguccuagga gacacagcau gggacuucgg uagcguaggc ggagccuuaa     1920

acucacuggg aaaaggcauc caccaaaucu ucggagccgc auucaaauca cuauucggag     1980

gaauguccug guucagucaa auacucaucg gcacgcuacu cauguggcua ggacucaaca     2040

ccaaaaacgg aagcaucucg cugaugugcc uagcccuagg aggcguacuc auauuccuau     2100

ccacagcggu cucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7364
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 2 opt

<400>  7364
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcgucggaau agugggacuc cuacucacaa cagcaauggc ggccgagguc acacgacgag      120

gaagcgccua cuacauguac cuagaccgca acgacgcagg agaggccauc ucguucccca      180

cgacccuagg caugaacaaa ugcuacaucc agaucaugga ccucggucac augugcgacg      240

caacaauguc guacgaaugc cccaugcucg acgaaggggu cgaaccagac gacguagacu      300

gcuggugcaa cacaacaagc accugggugg uauacggcac cugucaccac aagaaaggag      360

aagcccgaag aagccgcaga gccguaacac ucccaagcca cuccacccgg aagcuacagg      420

cacgcagcca aaccuggcua gaaagcagag aauacaccaa acaccucaua cgcgucgaaa      480

acuggauauu cagaaacccc ggauucgccc uagccgccgc cgccaucgcg uggcuuuuag      540

guuccuccac uagccaaaaa guaauauacc uaguaaugau acuccuaaua gcgccagccu      600

acagcaucag augcauaggc guauccaaca gagacuucgu cgaaggcaug uccggaggaa      660

caugggucga ugucguacua gaacacggcg gcugcguaac cgucauggcc caagacaaac      720

caacaguaga uauagaacuc gucacuacca cgguaagcaa cauggccgag gugcgcucau      780

acugcuacga ggccagcauc ucagacaugg caucagacuc acgcugccca acacaaggag      840

aagcauacuu agacaaacaa agcgacacac aauacguaug caaacgcacc cuaguagaua      900

gaggcugggg caacgggugc ggacucuucg gcaaaggauc ccuagucaca ugcgcgaaau      960

ucgccugcuc aaagaaaaug acaggaaaau caauacaacc agaaaaccuc gaauaccgca     1020

ucaugcuaag cgugcacgga ucacaacacu ccggcaugau cguaaacgac acaggccacg     1080

aaacagacga aaaccgagca aaaguggaaa uaacaccuaa cucaccacgc gccgaagcca     1140

cccuaggggg cuucggcuca cucggccucg acugcgaacc caggacaggc cuggacuuuu     1200

cggaccugua cuaccuuaca augaacaaca aacacuggcu aguacauaaa gaaugguucc     1260

acgacauucc acuucccugg cacgcaggag ccgacaccgg caccccacac uggaacaaua     1320

aggaagcacu cguagaguuc aaagacgccc acgccaagag acagacagua gucguacucg     1380

gaucccagga gggcgcagua cacacggccc uagcgggcgc acuagaagcc gagauggacg     1440

gcgccaaagg ccggcucagc agcggccacc ucaaaugccg gcuuaaaaug gacaagcuca     1500

gacugaaagg cguaucauac agccuaugca ccgccgccuu cacguucaca aaaaucccag     1560

cggaaacacu acacggaacc gucacagucg aagugcaaua cgcgggcaca gacggccccu     1620

gcaaagugcc cgcccaaaug gcggucgaca ugcaaacacu aaccccagua ggaagacuca     1680

ucaccgcaaa cccggucaua acagaaagca cagaaaacag caagaugaug cuagaacuag     1740

acccaccauu cggcgacuca uacauaguca ucggcguagg agaaaagaaa aucacacacc     1800

acuggcaccg aagcggcucu acaaucggaa aagccuucga agccaccgua cgcggcgcga     1860

aacgcauggc cguccucgga gacacagcau gggacuuugg cagcguagga ggcgcccuca     1920

acucccuagg caaaggcauc caccaaauau ucggggccgc cuucaaaucc cucuucggag     1980

gaaugucaug guucagccag aucuuaauag gaacccugcu aauguggcua ggccucaaca     2040

cgaaaaacgg guccauaucg cuaaugugcc uagcacuggg cggaguccuc auauuccuau     2100

caacagccgu auccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7365
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 2 opt

<400>  7365
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcgucggcau cguaggccuc cuccugacca cagccauggc agccgaaguu acacgccgag      120

gaucagcaua cuacauguac cucgaccgcu ccgacgcggg agaagccauc agcuucccua      180

ccacccucgg caugaacaaa ugcuacauac aaauaaugga ccugggacac augugcgacg      240

caacaauguc guacgaaugc ccaaugcuag acgaaggcgu cgaacccgau gacguagacu      300

gcuggugcaa caccacaucc accugggugg ucuacggaac cugccaccac aaaaagggcg      360

aggccagacg gucgagacgc gcggucaccc uuccuagcca cagcaccaga aaacuacaaa      420

cacggucgca gacuuggcuc gaaaguagag aguauaccaa acaccucauc cgggucgaaa      480

auuggaucuu ccgcaacccc ggauucgcgc ucgccgccgc cgcaauagcg uggcuacucg      540

gcagcuccac aagccaaaaa gucauauacc ucguuaugau acuccucauc gcccccgccu      600

acuccauucg augcauaggc guaagcaaca gagacuucgu agaaggcaug aguggaggga      660

ccugggucga cguaguccuc gaacacggcg gcugcgucac cguaauggcg caagacaaac      720

ccacaguaga caucgagcua gucaccacca ccguaaguaa cauggcagaa guccgauccu      780

acugcuacga agcaucaauc uccgacaugg ccuccgauag ccggugcccc acacaaggcg      840

aagcauaccu cgacaagcaa agcgacacuc aguacgucug caaaagaaca cucgucgacc      900

gaggcugggg aaauggaugc ggccuauucg guaaaggcuc ccucguaacc ugcgccaaau      960

ucgcauguuc gaagaaaaug accggaaaaa gcauacaacc cgagaaccua gaauaccgua     1020

uaaugcuaag cguccacgga agccaacaca gcggaaugau cgucaacgac acaggccaug     1080

aaacagacga aaaccgcgcc aaaguagaaa uaaccccuaa cucaccaagg gcagaagcaa     1140

cccuaggcgg auucggaagc cuaggacucg acugugaacc aaggacagga cucgacuucu     1200

cagaccuaua cuaccucacc augaacaaca aacacuggcu gguccacaaa gaaugguucc     1260

acgacauucc gcuaccgugg caugcuggcg ccgauacagg cacgccccau uggaacaaca     1320

aagaagcacu cguagaguuc aaagacgccc acgcaaaacg ccaaaccguc gugguccuag     1380

ggagccagga aggagccguc cacaccgccc ucgccggagc ccucgaagcc gaaauggacg     1440

gcgcaaaagg ccgcuuaagc uccggccacc uaaaaugccg ccugaaaaug gacaaacuca     1500

ggcucaaagg cguaagcuac ucgcuaugua ccgcagcauu cacauucagc aaaaucccag     1560

cagaaacacu acacggcaca gucacagaag aaguacagua cgccggcacc gacggccccu     1620

gcaaaguccc cgcgcaaaug gccgucgaua ugcaaacacu aacacccgua ggccgacuaa     1680

uaacagcaaa ccccguaauc accgaaagca ccgaaaacag caaaaugaug cuggaacucg     1740

acccgcccuu cggagacuca uacaucguaa uaggcgucgg agaaaagaaa aucacucacc     1800

acuggcaccg cuccggcagc accaucggca aagccuucga agccaccguc cgcggcgcca     1860

agcgaauggc gguacuaggc gacacagccu gggacuucgg aucaguugga ggagcccuaa     1920

acucacuagg caagggcaua caccaaaucu ucggagcagc cuucaaaucc cuauucgggg     1980

gcaugagcug guucagccaa auccuaaucg gaacccuccu cauguggcuc ggccuaaaca     2040

ccaaaaacgg cuccauuagu cuaaugugcc uagcccucgg cggcgugcuc aucuuccuau     2100

ccacagcggu aucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7366
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 2 opt

<400>  7366
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

cagucggaau aguaggacua cuccucacga cagcaauggc cgcagaagua accagacgcg      120

guuccgccua cuacauguac cucgaccgcu ccgacgccgg agaagccauc agcuucccca      180

caacccuagg caugaacaaa ugcuacauac aaaucaugga ucucggacac augugcgacg      240

ccaccauguc cuacgaaugc cccaugcucg acgaaggagu agagccggac gacguugacu      300

gcuggugcaa cacaacaucc acguggguag ucuacggaac cugccaucac aagaaaggcg      360

aagcccgaag aucccgccgc gccgucacac uacccagcca cucaacgcgg aaacuccaaa      420

caagaucgca aaccuggcua gaaagcagag aauauaccaa acaccuaauc agaguagaga      480

acuggauauu caggaaccca ggcuucgcac uagccgccgc agccauagcc uggcuccucg      540

guuccuccac aagccaaaaa guaauauacc ucgucaugau acuccucauc gcaccagcau      600

auucgauacg uugcauagga gugagcaaua gagacuucgu agagggaaug ucaggaggca      660

ccuggguaga cgucguacuc gagcacggcg gauguguaac aguuauggcc caagacaaac      720

ccaccgucga cauagaacuc gucacaacca cagucucaaa cauggccgaa guaagauccu      780

acugcuacga ggcuuccauc ucagacaugg cauccgacuc acgcugccca acccaaggcg      840

aagcguaucu cgacaaacag ucugacaccc aguacgucug caaacgcaca cucguggaca      900

gaggaugggg caacggaugc ggccucuucg gcaaaggcuc ccuaguaaca ugcgccaaau      960

ucgcaugcuc caagaaaaug accggaaaaa gcauacagcc cgaaaaccuc gaauaccgca     1020

uaaugcuauc aguccacgga ucccaacacu ccggcaugau cguaaacgac accggccacg     1080

aaaccgacga aaaccgcgcg aaagucgaaa ucacccccaa cagcccacga gccgaagcca     1140

cacuaggugg cuucggaagc cucggacugg acugugaacc ccgcacaggc cuagacuuca     1200

gcgaccuuua cuaccucacc augaacaaca aacacuggcu cguacacaaa gaaugguucc     1260

acgacauacc acuaccgugg cacacaggag cagacaccgg aacaccacac uggaacaaca     1320

aagaagccuu aguagaguuc aaagacgccc acgcaaaacg acaaacaguc guaguacucg     1380

gcucucaaga aggagccguc cacaccgcac uagcaggcgc ccucgaagcc gaaauggacg     1440

gagcuaaagg caggcugagc ucgggccacc uuaaaugcag acucaaaaug gacaaacuua     1500

gacucaaagg aguaucauau ucccucugca cagcagcauu caccuuuacc aaaauaccag     1560

ccgaaacccu acacggaacc gugaccguag aaguucaaua cgcaggaacc gacggcccau     1620

gcaaaguacc cgcacaaaug gcaguggaca ugcagacccu gacaccgguc ggacgccuca     1680

uaacagccaa ccccgucauc acugaaggca cagaaaacuc gaaaaugaug cucgaacuag     1740

acccacccuu cggcgacucc uacauaguca ucggcgucgg ugaaaagaaa aucacacacc     1800

acuggcacag aagcggcuca acaaucggaa aagccuucga agccaccguc cgcggugcga     1860

aaagaauggc aguccucggc gacacagcau gggauuucgg aagcguaggc ggaguacuaa     1920

acucgcucgg caaaggcaua caccaaaucu uuggcgcagc cuucaaaagc cuauucggag     1980

gcauguccug guucagccag auccuaaucg gcacacuccu aauguggcug ggacucaaua     2040

ccaaaaacgg gagcauaucc cucaugugcc uugcacuagg cggcguucuc aucuuccucu     2100

ccacagccgu cagcgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7367
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 2 opt

<400>  7367
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucgguau agucggcuua cuccucacaa cagcaauggc agccgagguc acccgaagag      120

gaucagccua cuacauguau cuggaccgau cagacgcagg ggaagcaauc uccuucccaa      180

caacccuagg caugaauaag ugcuacauac aaauaaugga ccucggacac augugcgacg      240

ccacgauguc cuacgaaugc ccaaugcuug acgagggcgu agaaccagac gacgucgacu      300

guuggugcaa caccacgucc acgugggucg ucuacggaac cugccaccau aagaaaggcg      360

aagcgcgacg uagcagacga gcagucacuc uaccaagcca cagcaccaga aaacuacaaa      420

caagaagcca aaccuggcua gaaagccgag aguacaccaa acaccuaauc cgcguagaaa      480

acuggaucuu caggaacccu ggcuucgcac ucgccgcagc agcaaucgcc uggcugcucg      540

gaucaagcac aagccaaaaa guaauauacc uugugaugau ccugcuaaua gcccccgccu      600

acuccauacg cugcauagga gucucaaauc gcgacuucgu ugaaggcaug uccggcggca      660

caugggucga cguagugcuc gaacacggug gcugcgucac agucauggcc caggacaaac      720

caacaguaga cauagaacuu guaaccacua ccgucaguaa cauggccgag guacguucau      780

acuguuauga agcauccaua ucggacaugg ccucagacuc ccgaugccca acccaaggcg      840

aagcguaccu cgacaaacaa ucagacacac aauaugucug caaacgcacc cuagucgaca      900

gaggcugggg aaacggaugc ggacucuucg gaaaaggcuc ccucguaacc ugcgcaaaau      960

ucgcgugcuc aaagaaaaug acgggcaaaa gcauacaacc cgaaaaccuc gaguaccgca     1020

ucaugcuauc aguccacggu ucacagcaua gcggcaugau aguaaacgac acaggacacg     1080

agacagacga gaaccgcgca aaagucgaga uuacaccuaa uaguccccgc gcagaagcca     1140

cauuaggcgg auucggaagc cugggacuag acugcgaacc ucgaaccgga cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca aacacuggcu gguccacaaa gaaugguucc     1260

acgacauccc cuuaccaugg cacgccggcg cggacacagg caccccacac uggaacaaca     1320

aagaagcccu agucgaguuc aaagacgccc acgccaaaag acaaacugua gucguccuag     1380

gaagccaaga aggagcagug cacaccgcac uggcaggcgc ccuagaagca gaaauggacg     1440

gcgccaaagg cagacugagc ucaggccacc uaaaaugccg acucaaaaug gauaaacucc     1500

gauuaaaagg ggucucauac ucccucugca ccgccgccuu cacuuucaca aaaauccccg     1560

ccgaaacccu ccacgguacc gucaccgucg aaguacaaua cgccggcacc gacggccccu     1620

gcaaaguccc cgcccaaaug gccguagaca ugcaaaccuu aaccccaguc ggacgccuca     1680

ucacagcaaa ucccgucauc accgaaucca ccgaaaacuc gaaaaugaug cucgaacuag     1740

acccaccauu cggagacagc uacaucguca ucggaguagg ugaaaagaaa auaacccacc     1800

acuggcaccg cagcggaagc accauaggaa aagccuucga ggccaccguc cgaggagcaa     1860

aaagaauggc gguucucggu gacaccgcau gggacuucgg cagcguaggc ggagcucuga     1920

acagccuagg aaaaggaauc caccaaauuu ucggggccgc auucaaaagc cucuucggag     1980

gaauguccug guucucgcaa auacucauug gcacacuccu cauguggcuc ggacucaaca     2040

caaagaacgg cagcaucagc cucaugugcc ucgcccuugg uggcguccuc auauuccuca     2100

gcacagccgu cucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7368
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 2 opt

<400>  7368
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau agucggacuc cuacucacca ccgccauggc cgucgaaguc acgcgccggg      120

gcaacgccua cuacauguac cuagaccgua gcgacgcagg agaggcaaua ucauucccca      180

caacaauggg aaugaacaaa ugcuacaucc agaucaugga ccuaggccac augugcgacg      240

caacaauguc auacgaaugc ccaaugcucg acgaaggagu ggaacccgac gacguagacu      300

gcuggugcaa cacaacaagc accuggguag uauacggaac gugccaccac aagaaaggcg      360

aagcccgacg gucccggcga gccgucaccc ucccaagcca cuccacacga aaauuacaaa      420

caaggagcca aaccuggcuc gaaagccgag aguacaccaa acaccucauc agaguggaaa      480

acuggaucuu ccgaaacccu ggauucgcgc ucgccgcagc agccauagca uggcuucucg      540

gcuccaguac cagccagaaa guuaucuacc ucguaaugau ccuacucauc gcuccagcau      600

acuccauccg augcaucggc gucagcaacc gcgauuucgu cgaagguaug agcggcggca      660

cauggguaga cgucguacua gaacacggcg gcugugugac agucauggcu caggacaaac      720

caacagucga uaucgaacuc guaaccacaa ccgucuccaa uauggcagaa gucagaucau      780

auugcuacga agcaagcauc uccgacaugg ccagcgacuc acguugccca acccaaggcg      840

aagccuaccu ggacaaacag uccgacaccc aauacguaug caaacgaacc cucguugacc      900

gaggcugggg caacggcugc ggccucuucg gcaaaggauc acucguaaca ugcgccaaau      960

ucgcuugcuc aaagaaaaug acgggaaaaa gcauacaacc cgaaaaccua gaguaccgua     1020

uaaugcucuc cguccaugga ucccaacacu caggcaugau cgucaacgac acgggccacg     1080

aaacagacga aaaccgcgca aaagucgaaa uaacacccaa cagcccccga gccgaagcaa     1140

cacucggagg cuucgguagc cucggccuag acugugaacc gcgcacagga cuagacuuua     1200

gcgaccucua cuacuuaacc augaacaaca aacacuggcu cguccacaaa gaaugguucc     1260

acgacauacc auugcccugg cacgcagggg ccgacacagg caccccacac uggaacaaca     1320

aagaagcccu cguagaguuc aaagaugcac acgcuaaaag acaaaccguc gucguacucg     1380

gcagccaaga aggcgccgua cacacagccc uugcgggcgc acuagaagca gaaauggacg     1440

gagccaaagg ccgacuguca uccggccacc ucaagugcag acucaaaaug gacaaacucc     1500

gccuaaaagg cguuuccuac agccucugca ccgccgccuu cacauucacc aagauucccg     1560

cggaaacucu ccaugggacc gucacaguag aaguacaaua cgcaggaaca gacggccccu     1620

gcaaaguccc cgcccagaug gccgucgaca ugcaaacccu gacccccguc gggcgucuaa     1680

ucacagccaa cccgguaauc accgaaucga ccgaaaacuc aaaaaugaug cuagaacuug     1740

acccgcccuu cggcgacucc uacaucguca uaggcguagg cgaaaagaaa auaacccauc     1800

acuggcaccg auccgguagc accauaggga aagccuucga agccaccgua cgcggagcaa     1860

aaagaauggc cguccucgga gacacagcau gggacuucgg aucgguaggc ggagcacuca     1920

auucacuagg caaaggcaua caccagaucu ucggcgcagc auucaaaagc cucuucggcg     1980

gaaugagcug guucagccaa auccuaauag gaacccuacu gguuuggcua ggccucaaca     2040

ccaaaaacgg cucaaucagc cucaugugcc uugcccuagg aggcguccuc auauuucuau     2100

caacugcagu cucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7369
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 2 opt

<400>  7369
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguaggcau agucggacuc cucuugacca cagccauggc agccgaaguc acccgacgcg      120

gcagcgcaua cuacauguac cuagaccguu ccgacgcagg cgaagcaauu agcuucccca      180

ccacccuagg caugaacaaa ugcuacauac aaauaaugga ucucgggcac augugcgaug      240

ccacaauguc guacgaaugc cccaugcucg acgaaggagu agaacccgac gacgucgacu      300

gcuggugcaa cacaaccagu acauggguag ucuacggaac cugccaccac aagaaaggug      360

aagcucggag aucccgacgc gcggucaccc uccccuccca cuccacaaga aaacugcaaa      420

caaggucaca aacauggcuc gaaagcagag aauacaccaa gcaccuaaua agaguggaaa      480

acuggauauu ucggaacccc ggcuucgcgu uagccgcagc cgccaucgcg uggcuccuag      540

ggagcucaac uucacaaaaa guaauauacc ucgucaugau ccuccugaua gccccagccu      600

acuccauacg uugcauaggc guaucuaacc gcgacuucgu cgaaggaaug uccggaggca      660

caugggucga cgugguacuc gaacauggcg gcugcguaac cguaauggca caagacaaac      720

cgacgguaga cauagaacug guuacaacca ccgucagcaa cauggccgaa guaagaucau      780

acuguuacga agcaucaauc agcgacaugg ccagcgacuc ccgcugcccc acccaaggag      840

aagcguaucu cgacaagcaa uccgacaccc aauauguaug caaacgcacc cucguggacc      900

gaggaugggg caacggaugc ggccuauucg gcaaaggauc ccuagucaca ugcgcgaagu      960

ucgcaugcuc aaagaaaaug accggaaaau ccauacaacc cgagaaucuc gaauacagaa     1020

ucaugcucuc cguacacggg ucucaacauu ccggcaugau cgugaacgac acuggccacg     1080

aaaccgauga aaaccgagca aaagucgaga uaacaccaaa uucaccccgc gccgaagcca     1140

cccuaggagg cuucggaagc cuaggccucg acugcgaacc ccgaaccgga cuggacuucu     1200

ccgaccugua cuaccucaca augaacaaca aacacuggcu aguccacaaa gagugguucc     1260

acgacauacc acuucccugg cacgccggcg ccgauacagg gacaccccac uggaacaaca     1320

aagaagcccu uguagaguuc aaagacgcac acgcaaaacg ccaaacaguc gugguccuag     1380

gcagccaaga gggagccgua cacaccgccc uagccggagc ccuagaagcc gaaauggacg     1440

gcgccaaagg cagauuaagc uccggccacc ucaagugccg acuaaaaaug gauaaacugc     1500

gccuuaaagg cguaagcuac ucccucugua cagcagcauu cacauucaca aaaauacccg     1560

ccgagacacu ccacggaaca guaacagucg aaguccaaua cgcaggaaca gauggacccu     1620

gcaaaguccc ggcacaaaug gccguagaca ugcaaacccu aacccccguc ggcagacuca     1680

uaaccgcaaa ccccgucaua acagagucaa ccgaaaacuc aaaaaugaug cuggaacucg     1740

acccacccuu cggcgacagc uacauaguaa uaggcguagg cgaaaagaaa auaacacacc     1800

acuggcaccg cagcggauca accaucggga aagccuucga agccacgguc cgaggcgcaa     1860

aaagaauggc cgugcucggc gacacagccu gggacuucgg cagcgucggc ggagcccuca     1920

acucccuagg caagggcauu caccaaaucu ucggagccgc cuucaaaagc cucuucggag     1980

gcaugucgug guucucccaa auacuaaucg gcacccuccu uauguggcug ggccucaaca     2040

ccaaaaacgg cuccaucucg cuagucugcc uagcccucgg gggaguccuc auauuccuuu     2100

cgacagcagu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7370
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 2 opt

<400>  7370
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

caguuggaau aguggggcua cuacuaacca cagccauggc ggcagaagua acccgaagag      120

gcagcgccua cuacauguac cucgaccgca gcgacgccgg cgaagcaauc agcuucccaa      180

ccacccuagg aaugaacaaa ugcuacaucc aaaucaugga ccuaggucac augugcgacg      240

ccaccaugag cuacgaaugc cccaugcuug acgaaggagu ugaaccagac gacgucgacu      300

gcuggugcaa caccacaagc accugggucg ucuacgggac cugccaccac aagaaaggcg      360

aagcgcgccg aagccgcaga gcugucaccc uccccagcca cuccacccga aaacuacaaa      420

ccaggagcca aacauggcuc gaaagccgcg aauauaccaa acacuuaauc agaguggaga      480

acuggauauu ccgcaacccc ggauucgcac ucgcagcggc agcgaucgca uggcuccuag      540

gcagcuccac cagccaaaaa gucaucuacu uagucaugau ccuccucaua gcccccgccu      600

acucgaucag augcaucggc guuaacaacc gagacuucgu cgaaggcaug ucuggcggca      660

ccuggguuga cgucguccua gagcacggcg gcugcguaac cguaauggcu caagacaaac      720

ccaccgucga caucgagcua gucacaacca ccguaagcaa cauggccgaa guucgaagcu      780

acugcuacga agcauccauu agcgacaugg caucagacuc aaggugccca acacaaggag      840

aggcuuacuu agacaaacaa agcgacacgc aauacgugug caaacgcacc cuagucgacc      900

gcggaugggg caacggcugc ggccucuucg guaagggcag ccugguaacc ugcgcaaaau      960

ucgccugcuc aaagaaaaug acaggaaaaa gcauacagcc cgaaaaucuc gaauaccgua     1020

uaaugcuaag cguccacgga ucacaacaca gcggaaugau cgucaacgac accggacacg     1080

aaacagauga aaaccgagcc aaagucgaaa ucaccccaaa cagcccccgc gcugaagcca     1140

cccugggcgg guucggauca cuaggccuag acugcgagcc gcgcacaggc cuggacuuca     1200

gcgaccucua cuaccuaacc augaacaaca aacacuggcu cguacacaaa gaaugguucc     1260

acgacauccc acucccaugg cacgccggcg cagacaccgg aaccccgcac uggaacaaca     1320

aagaagcacu cgucgaguuc aaagacgccc acgccaaacg ccagacaguc gucguccugg     1380

gaucccaaga aggcgccguc cacacagcac ucgccggcgc ccuagaggcc gaaauggacg     1440

gagcaaaagg acgccuauca aguggccauc ucaaaugccg gcuuaaaaug gacaaacuua     1500

gauugaaagg cgucuccuac ucucucugua ccgcggccuu cacauucaca aaaauacccg     1560

cggaaacacu ccacggcaca gucaccgucg agguccagua cgcagggacc gaugggccgu     1620

gcaaaguacc agcccaaaug gccguagaca ugcaaacacu aacccccguc ggacgucuaa     1680

uaacagccaa ccccgucaua acggaaucaa cagagaacuc caaaaugaug uuagaauuag     1740

acccuccauu cggcgacucg uacaucguca uagggguagg agaaaagaaa aucacacacc     1800

acuggcaccg cagcggaagc accauaggca aagcguucga agccaccgua cgaggcgcca     1860

aacgcauggc cguccucgga gauaccgccu gggacuucgg aagcgucggc ggagcccuca     1920

acucucuagg aaaaggcauc caccaaauau ucggagccgc auucaaaagc cuauucggag     1980

gcaugucaug guucucccaa auacucaucg gaacgcucuu aauguggcua ggccucaaua     2040

ccaaaaacgg cuccauaucc cuagucugcu uagcccucgg cggcguccua auauuccuaa     2100

gcaccgcagu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7371
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 2 opt

<400>  7371
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguagggau cgucggucug cuccugacca cagcaauggc aguggaaguc acacgacgcg      120

gcagcgcgua cuacauguac cucgaccgau cagacgcagg cgaagcaauc uccuucccca      180

ccacacucgg caugaacaaa ugcuacaucc aaaucaugga ccuaggacac augugcgacg      240

caacaauguc auacgaaugc ccaaugcugg acgaaggagu agaacccgac gacgucgacu      300

gcuggugcaa cacuaccagc acaugggucg uauacgggac uugccaucac aagaaagggg      360

aagcaagacg cucacgacga gcggucacgc ucccgagcca cucaacacgg aaacuccaaa      420

cucggucgca aaccuggcua gaaucaagag aguacacaaa acaccuaaua cgaguagaaa      480

acuggaucuu cagaaaccca ggcuucgccc ucgccgcggc cgccaucgcc uggcuccuag      540

gcagcagcac cucccaaaaa gucauauacc ugguaaugau ccuacugaua gcaccagccu      600

acucaaucag gugcauagga guaagcaacc gagauuucgu agaaggaaug uccggaggca      660

cauggguaga cgugguccug gaacacggcg gaugcgugac agucauggcc caagacaaac      720

cagccgucga uauagaacuc gucaccacaa ccguaagcaa cauggcagaa guaagauccu      780

acugcuacga agccagcaua agugacaugg caagcgacag ccgcugcccc acccaaggcg      840

aagccuaccu cgacaaacaa ucagacaccc aguacguaug caaacgcaca uuaguagaca      900

gaggcugggg caacggcugc ggacuauucg ggaaaggcuc gcugguaacc ugcgccaaau      960

ucgcgugcuc caagaaaaug accggcaaaa gcauacagcc cgaaaaccuc gaauaccgga     1020

uaauguuguc cgugcacggc agccaacacu ccggcaugau cgucaacgac accggacaug     1080

agacagacga aaaccgcgcc aaaguagaaa uaaccccuaa cagcccgcga gccgaggcga     1140

cccucggagg uuucggcucg uuaggccucg auugcgaacc ccguaccgga cuagacuucu     1200

ccgacuuaua cuaccucacg augaacaaca aacacuggcu uguacacaaa gaaugguucc     1260

acgacauacc acugcccugg cacgccggcg cagacacagg caccccacac uggaacaaca     1320

aagaggcccu aguagaauuu aaagacgccc augcaaaacg ccaaacagua guaguccucg     1380

gcagccaaga aggcgcagug cacacagcac uagccggagc ccuagaggcc gaaauggacg     1440

gcgcaaaagg gcgacuaagu agcggacacc uaaaaugccg gcucaaaaug gacaaacucc     1500

gccucaaagg cgucuccuac ucccuaugca ccgcagcauu uacauucaca aaaauccccg     1560

cagaaacccu acauggaacc gugacaguag aaguccaaua cgcaggaacg gacgggccau     1620

gcaaagugcc cgcacaaaug gcaguagaca ugcaaacacu cacaccaguc ggcagacuaa     1680

ucaccgcgaa ucccgugaua accgaaucaa ccgaaaacag caaaaugaug cuagaacuag     1740

acccacccuu cggcgacagu uacauaguca uaggcguagg agaaaagaaa aucacacacc     1800

acuggcaccg auccggauca acaauaggaa aagcguucga agcgaccgua cgcggagcca     1860

aacgaauggc aguucucgga gacacagccu gggacuucgg aagcguaggc ggcgcgcuaa     1920

acagccucgg caaaggcauc caucagaucu ucggagcagc guucaaauca cuauucggag     1980

ggaugagcug guucagucag auacuaauag gcacauuguu aguguggcuc ggccuaaaca     2040

cgaaaaacgg cagcaucagc uuaaccugcc ucgcacucgg cggcguacuc auuuuucuaa     2100

gcaccgcagu aucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7372
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 2 opt

<400>  7372
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

cagucggcau cguaggccuc cuucuaacaa ccgcaauggc cguagaggua acgcgucgag      120

gcucagcgua cuacauguac cuggaccgau cagacgcggg ggaggcgaua ucguucccaa      180

ccacgcucgg aaugaacaaa ugcuacauac aaauaaugga cuuaggacac augugcgacg      240

ccaccauguc cuacgaaugc cccaugcucg acgaaggagu ggaaccagac gacgucgacu      300

gcuggugcaa cacaaccagu accuggguag ucuacggcac gugccaccac aagaaaggcg      360

aagcccgccg cucccgccga gcugucacac ucccaagcca uagcacacgc aaacugcaga      420

ccagaagcca aaccuggcuc gaaucuagag aauauacaaa acaccugauc cgcguagaga      480

acuggauauu cagaaaccca ggauucgccc uagccgcagc agucaucgcc uggcuacucg      540

gauccagcac aucacagaag guaauauacc ucguaaugau auuacucauc gcaccagcgu      600

auucgauccg gugcaucggc guaagcaacc gagacuucgu ugaaggaaug ucaggcggga      660

ccugggucga cgucguccug gaacacggag gaugcguuac ggucauggca caagacaaac      720

ccacagucga caucgaacuc gucacaacga ccguaaguaa cauggcagaa guacgguccu      780

acugcuacga agcuagcaua ucagacaugg cuucagacuc cagaugcccc acacagggcg      840

aagccuaccu agacaaacaa ucagacacgc aauacgucug caaacgaacc cucguagacc      900

gaggcugggg aaacggcugc ggccucuucg gcaaaggauc gcucgugacc ugcgccaagu      960

uugcaugcuc caagaaaaug acaggcaaau ccauacaacc cgagaaccuc gaauaccgca     1020

ucaugcucag cguccacgga ucacaacacu cgggaaugau agucaacgac accgggcacg     1080

agaccgacga aaaccgcgcc aagguggaaa uaacaccuaa cagucccaga gcagaagcca     1140

cacucggcgg auucggguca cucggccucg acugcgaacc acgaaccgga cuagacuuca     1200

gcgaccucua cuaccugaca augaacaaca aacacuggcu uguacacaaa gaaugguucc     1260

acgauauacc acucccaugg cacgccgggg ccgauaccgg uaccccacac uggaacaaca     1320

aagaggcacu cguagaguuc aaagacgcac acgcaaaacg ccaaaccguc gucguacucg     1380

ggucacaaga aggcgccgug cauacagccc ucgcuggcgc ccuagaagcc gagauggacg     1440

gcgcuaaggg ucgacuuucc ucaggccacc uaaaaugccg ccugaaaaug gacaaacucc     1500

ggcucaaagg ggucuccuac ucacucugca ccgcagccuu cacuuucacc aagauacccg     1560

ccgaaacccu ccacggcacc gucaccgucg aaguccaaua cgcaggaacc gacggacccu     1620

gcaaaguacc cgcucaaaug gcgguagaca ugcaaacccu gacgcccguc ggccgccuaa     1680

uuaccgccaa uccugucaua accgaaucga cagaaaacag caaaaugaug cucgaacugg     1740

acccacccuu cggcgacagc uacauaguca ucggcgucgg cgaaaagaaa aucacccacc     1800

acuggcaccg auccgguagc accauaggaa aagcauucga agccaccgua cggggagcaa     1860

aaagaauggc gguacugggc gacaccgcau gggacuucgg gagugucgga ggagcccuua     1920

acucguuagg caaaggcaua caccagauau ucggcgccgc cuucaaaagc cuguucggcg     1980

gcaugagcug guucagccaa auccuaaucg ggacacuccu ggucuggcua ggccucaaca     2040

ccaagaacgg auccauuagc cuaaccugcc uggcacucgg aggaguucua auauuccuca     2100

gcaccgccgu cagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7373
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 2 opt

<400>  7373
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gcguaggcau aguaggccuc cuacuaacca ccgcaauggc cguagaggua acccgccgag      120

gcucagccua cuacauguac cuagaccgaa gcgacgccgg cgaagccauc agcuuuccca      180

cgacccuagg agucaacaaa ugcuacauac aaauaaugga ccucgggcac augugcgacg      240

caaccauguc auacgagugc ccaaugcuag acgaaggagu cgaacccgau gacgucgauu      300

gcugguguaa caccaccuca acgugggucg ucuacggaac augccaccac aagaaaggcg      360

aagcacggcg gagcagacga gcagucacgc uaccauccca cucaacacgc aaacuccaaa      420

cccgaucaca aacauggcuc gaaucccgcg aauacaccaa acaccuaauc agaguagaaa      480

acuggaucuu ucgcaacccc gguuucgcac ucgcagcagc cgcuaucgca uggcuacuag      540

gcucauccac cucacagaaa gucauuuacc ucgugaugau ccuacuaauc gcacccgcau      600

acucgauccg cugcauagga guuagcaacc gcgacuucgu agaaggaaug agcggcggca      660

cauggguaga cguagugcua gaacacggcg gcugcgucac cgucauggcc caggacaaac      720

ccacgguaga caucgaauua gucacuacaa cggucagcaa cauggcagaa guccgcucau      780

acugcuacga ggcaagcauc uccgacaugg cuuccgacag ccgcuguccc acgcagggcg      840

aagccuaccu agacaaacaa uccgacacac aauacgucug caaaaggacc cuaguagaca      900

gaggaugggg aaacgggugc ggacucuucg gaaaaggcag ccucgugacc ugcgccaaau      960

ucgccugcuc aaagaaaaug accggaaaaa gcauccaacc cgaaaaucuc gaguacagaa     1020

ucaugcucag cguccacgga ucacaacaca gcggcaugau agucaacgac acggggcacg     1080

aaacggauga aaaccgcgcc aaagucgaaa ucacccccaa cagcccccga gccgaagcca     1140

cccucggcgg guucggaucg cuaggacucg acugcgaacc acgaacaggc cuggacuuca     1200

gcgaccucua cuaccuaacg augaacaaua aacacuggcu aguacacaaa gaaugguuuc     1260

acgacauccc acuccccugg cacgccggag ccgacaccgg aacaccccac uggaacaaca     1320

aagaagcacu gguugaguuc aaggacgcac acgcaaaacg ccagaccgug guugugcuag     1380

gcucacaaga aggcgcagua cacaccgccc uggcaggagc ucuagaagcc gagauggacg     1440

gagccaaagg gcgccucagc uccggacacc ucaaaugccg ccuaaaaaug gacaaacucc     1500

gacuaaaagg agucucuuac ucacucugca ccgccgcauu caccuucacc aaaauacccg     1560

cagagacccu ccacggcaca gugaccgucg aaguacaaua cgccggaaca gacggcccau     1620

gcaaaguacc ggcccaaaug gccguagaca ugcaaacacu caccccaguc ggcagacuaa     1680

ucacagcaaa ccccgucaua acagaaucca cagagaacag caaaaugaug cucgaacuag     1740

auccgcccuu cggcgauagc uacauaguaa ucggugucgg agacaagaag aucacccacc     1800

acugguaccg auccgguagc accaucggca aagcauucga agccaccguc cgaggcgcca     1860

aaagaauggc aguccucggc gacaccgcgu gggacuucgg cagcguagga ggagcccuca     1920

acucccucgg aaaaggcauc caccaaaucu ucggcgccgc cuucaaaagc cuauucggug     1980

gaaugagcug guucucccag auccugauug gcacgcuucu aguauggcua ggccuaaaca     2040

cgaagaacgg cagcauaucc cucacgugcc uugcgcuagg cggcguacua auauucuuaa     2100

gcaccgccgu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7374
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 2 opt

<400>  7374
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucggcau cgugggccuc cuccuaacua ccgccauggc cgucgaaguc acacgccgcg      120

gcagcgcgua uuacauguac cuggacagaa gcgaugccgg aaaagccaua ucauucccca      180

ccacccuagg aguuaauaaa ugcuacauac aaaucaugga ccucggccac augugcgacg      240

caaccauguc cuacgaaugc ccaaugcuag acgaaggcgu agaaccugac gacguggacu      300

gcuggugcaa caccacauca accugggucg uuuacggcac augccaccac aagaaagggg      360

aagcccgacg cuccaggcgc gcggucaccc ucccaucaca cucaacccgc aaacuacaaa      420

cacgauccca gacuuggcua gaaucgcgcg aauacaccaa acaccucauc agagucgaga      480

acuggaucuu ccgaaacccc ggcuucgcac uagcagccgc ugccauagca ugguuauuag      540

gcucgagcac cucacagaaa gugauauauc ucgucaugau acugcucauc gcgccagcgu      600

acuccauucg gugcauaggc guaagcaaca gagacuucgu cgaaggcaug uccggcggaa      660

ccuggguaga cgucguccuc gaacacggug gcugcgugac cguaauggcc caagacaaac      720

ccacaguaga caucgaacug guuaccacaa ccguauccaa cauggccgaa gugcgcucau      780

auugcuauga agccagcauc ucagacaugg caucggacuc ccgcugccca acacaaggag      840

aagcguaccu cgacaaacaa agcgacaccc aguacgucug caaaaggaca cucgucgaca      900

gaggcugggg caacggaugc ggccucuucg gcaaaggcag ccucguaacc ugcgccaaau      960

uugccuguag caagaaaaug accggcaaaa gcauacaacc cgaaaaccuc gaauaccgga     1020

uuaugcucag cguccacggc ucacaacaca gcggcaugau cgucaaugac auaggccacg     1080

agaccgacga aaaccgcgcc aaagucgaga ucacccccaa cuccccacgc gcagaggcaa     1140

cucucggggg auucggcucc cucggcuuag acugcgaacc ccgaaccgga cucgacuucu     1200

cagaccugua cuaccucacc augaauaaca aacacuggcu aguccacaaa gaaugguucc     1260

acgacauacc ccuccccugg cacgcaggcg cggacaccgg aacaccgcac uggaacaaca     1320

aagaggcccu cgucgaguuc aaagacgcac acgccaaacg ucaaacagua gucguacucg     1380

gcucccaaga aggcgccgua cauaccgcac ucgccggcgc ccuagaagca gaaauggacg     1440

gcgccaaagg acgucuaagc uccggccacc ucaaaugcag acugaaaaug gauaaacuac     1500

gccucaaagg cgucagcuac ucccucugca cagccgcuuu cacauuuacc aaaaucccag     1560

ccgaaacauu acacggaacu guuaccguag aaguccagua cgccggcacc gacggcccau     1620

gcaaaguccc agcccaaaug gcagucgaua ugcaaacacu uacaccaguc ggacgccuca     1680

ucacagcaaa cccgguaaua accgaaucaa ccgaaaacuc caaaaugaug cuagagcucg     1740

aucccccuuu uggcgacagc uacaucguca ucggagucgg ggacaagaaa aucacccacc     1800

acuggcaccg cucaggcucc accaucggga aagcauucga agccacagua cguggugcca     1860

aacgcauggc cguacucggc gacacagcgu gggacuucgg cagcgucgga ggcgcacuca     1920

acucacucgg gaaaggcauc caccaaaucu ucggcgccgc auucaaaucc cuauucggcg     1980

gcaugucaug guucucccaa auacuaaucg gcacccuacu cgucuggcua ggacucaaca     2040

ccaaaaacgg aagcaucagc cucacgugcc uagcccucgg aggcguccuc auauuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7375
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 2 opt

<400>  7375
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcaucggcau cgucgggcuc cuacucacca ccgcaauggc cgcagaaauc acccgaagag      120

gaagcgccua cuacauguac cuggaccgga gcgacgcagg aaaagccauu agcuucgcca      180

ccacacuagg aguaaacaaa ugucacgugc agaucaugga ccuaggccac augugugacg      240

cgacaauguc cuacgaaugc ccaaugcugg augaaggagu agaacccgac gacguggacu      300

gcuggugcaa cacaacuagc acaugggucg ucuacggcac augccaccac aagaaaggag      360

aggcccgaag aucccggcgc gcagucaccc uaccguccca caguacccgc aaacuccaaa      420

ccagaagcca aacauggcua gaaagcagag aauacacgaa acaccucauc aaaguugaaa      480

acuggaucuu ccgaaacccc ggauucacgc ugguagcagu cgcaaucgca uggcuccuag      540

ggucuagcac uucacaaaaa guaauauacc ucgucaugau ccuucugaua gcgccggccu      600

acagcauacg cugcaucgga guauccaaua gagacuucgu agaggggaug uccggcggca      660

caugggucga cguaguccuc gaacauggag gcugugucac cguaauggcc caggacaagc      720

caaccguaga caucgagcuc guaacaacca ccguauccaa cauggcggaa guaagaagcu      780

acugcuacga agccagcauc agcgacaugg ccuccgacuc gcggugccca acccaaggcg      840

aagccuaucu cgacaagcaa uccgacacac aguacguaug caaaagaacc cucgucgauc      900

gcggcugggg caacgggugc ggccucuucg gaaaaggcag ccucguaacc ugcgcaaaau      960

uuaccugcuc caaaaagaug accggaaaau caauccagcc agaaaaccuc gaauaccgca     1020

ucaugcucag cguccacgga agccaacaua gcggcaugau cguaaacgac acaggacacg     1080

aaaccgacga aaaccgcgca aagguagaag uaacccccaa cagcccacgc gcggaagcga     1140

cgcuaggagg cuucggaagc cugggacucg acugcgaacc ccgcacgggc cuggacuucu     1200

cagaccucua cuaccucaca augaacaaca agcacuggcu aguccacaaa gaaugguucc     1260

acgacauccc acucccgugg cacgcgggcg cagacaccgg aacaccccac uggaacaaca     1320

aagaggcccu aguggaguuc aaagacgcac acgccaaacg acaaaccgua gucguccuag     1380

gcagccaaga aggcgccguc cacaccgccc ucgcaggcgc ccuagaagcc gaaauggacg     1440

gagcaaaagg acgacuauuc ucgggacacc ucaaaugcag acucaaaaug gacaaacuua     1500

gacuaaaagg ggucucauac agccucugua cagcagcauu caccuucacg aaaguaccgg     1560

ccgaaacacu acacggaacc guaacaguag agguacaaua cgcagggacg gacggcccgu     1620

gcaaaguccc agcccaaaug gcuguagaca ugcaaacccu aacaccaguc ggccgccuca     1680

uaaccgcaaa cccaguuauc acagaaucaa cagaaaacuc aaaaaugaug cuagaacuag     1740

acccacccuu cggagacagc uacauaguca uaggaguugg agacaagaaa auaacacacc     1800

acuggcaccg gucagggagc acaaucggaa aagccuucga agcaaccgua agaggcgcaa     1860

aaagaauggc gguccuuggc gacaccgcau gggacuucgg aucaguugga ggcguguuca     1920

acucacucgg gaaaggcaua caccaaauau ucggagcggc auucaaauca cuauucgggg     1980

gaauguccug guucagucaa auacucauag gcacccugcu aguauggcuu gggcuaaaca     2040

caaagaacgg auccauaagc cuaaccugcc ucgcacuggg aggcgucaug aucuuccuau     2100

caaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7376
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 2 opt

<400>  7376
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caauagggau cauaggccuc cuccugacaa ccgcaauggc ggcugagaua acgcgccgag      120

ggagcgccua cuacauguac cuagacagau cggacgcagg gaaagcaauc agcuucgcca      180

ccaacuuggg agucaacaaa ugccacgucc aaaucaugga ccucggacac augugcgacg      240

ccacaauguc uuacgagugc cccaugcuag acgaaggggu agaacccgac gacguggacu      300

guuggugcaa caccacaagc accuggguag ucuacggaac cugccaccac aagaaaggcg      360

aagcccggag aucccgccga gccguaaccc uccccuccca cucgacccga aaacuccaga      420

caagaaguca aaccuggcuu gaauccagag aauacacgaa acaccuaaua aaaguugaaa      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgcggu cgccauagcc uggcuccuag      540

gcagcucgac cagucaaaaa guaaucuacc uagucaugau ccuacucauc gccccggcau      600

acagcauacg auguaucgga gugagcaacc gagacuucgu cgaaggaaug agcggcggaa      660

cuuggguaga cguugucuua gagcacggcg gcugcguaac cgucauggcc caggacaaac      720

caaccgucga caucgagcua gugaccacca cggucucaaa cauggccgaa gugagaucgu      780

acugcuacga agccuccaua uccgacaugg caucagacuc gagaugccca acacaaggcg      840

aagccuaccu cgacaaacaa agcgacacac aauacgugug caaacgcacc cuaguagaca      900

gaggaugggg caacggaugc ggacucuucg gaaaaggcag ccuagucacu ugcgcgaaau      960

ucacaugcag caagaaaaug accggcaaaa gcauacaacc ggaaaaccua gaauaccgca     1020

ucaugcucag cguacacggu ucacaacacu ccggcaugau cgucaacgac auaggacacg     1080

aaaccgacga aaaccgcgca aaaguggaag ucaccccaaa cucaccgaga gcagaagcaa     1140

cacucggcgg auucggauca cuaggucucg acugcgaacc gcgcacuggc cuagacuucu     1200

ccgaccucua cuaccuuaca augaacaaca agcacuggcu aguccacaaa gaaugguucc     1260

augacauucc guugccaugg cacgcaggag ccgacacagg aacaccacac uggaacaaca     1320

aagaagcgcu aguagaguuc aaagacgccc acgccaagcg ucaaaccgug guaguccuag     1380

gaagccaaga aggcgccgug cacacagcac uagccggagc acucgaagcc gaaauggacg     1440

gugccaaagg gcgacuauuc agcggccacc ucaaaugcag gcucaaaaug gacaaacugc     1500

gacuaaaagg aguaucguac ucacuaugca ccgccgccuu cacauucaca aaaguaccgg     1560

ccgaaacccu acacggcaca guaaccgugg aaguccagua cgcaggaacc gacggaccuu     1620

gcaaaguccc cgcccaaaug gccgucgaca ugcaaacacu cacacccguc ggcagacuga     1680

uaaccgcaaa cccaguaauc accgaaagca cugagaacag caaaaugaug uuagaacuag     1740

aucccccauu cggcgacuca uacaucguca ucggagucgg cgacaagaaa auaacccacc     1800

acuggcaccg caguggcuca accaucggca aagcauucga agccaccgua cgcggcgcca     1860

aacgcauggc aguccucggu gacacagcau gggauuucgg aagcgucggc ggcgucuuca     1920

acucccucgg caaaggugug caccaaaucu ucggcgccgc cuucaagagc cucuucggag     1980

gcaugucgug guucucacaa auccuuaucg ggacacuccu agucuggcua ggacuaaaca     2040

ccaagaacgg caguaucagc cuaaccugcc ucgcccuagg cgggguaaug auauuccuaa     2100

gcacggccgu aagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7377
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 2 opt

<400>  7377
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucgggau cauaggccua uuacuaacca ccgcaauggc agccgagauc acccggcgcg      120

guagcgccua cuacauguac cucgauagau cagaugcagg caaagcaaua uccuucgcaa      180

ccacacucgg aguaaacaag ugccacgucc aaaucaugga ccuaggccac augugcgacg      240

caacaauguc auacgaaugc ccaaugcuag acgagggcgu cgaacccgac gacguagacu      300

gcuggugcaa caccacaagc acauggguag ucuacggaac augucaccac aagaaaggag      360

aggccaggag aagccgacgc gcagucaccc ucccaagcca cucgacacga aaacuccaaa      420

cacgcagcca aacauggcug gaaagccgcg aauacaccaa acaccucauc aaaguagaaa      480

acuggaucuu ccgcaaccca ggcuucgcac ucguagccgu agccauagcc uggcuccugg      540

gaagcagcac cagccaaaaa gucauauacc ucguaaugau ccuccugauc gcaccagccu      600

acucgaucag gugcauuggc gucagcaacc gagacuucgu cgaaggcaug uccggaggca      660

cauggguaga cgucgugcua gagcacggcg gcugcgucac ggucauggcc caagacaaac      720

ccacggugga cauugaacuc guaaccacca cagucagcaa cauggcugaa gucagaagcu      780

acugcuacga ggcaucgauc agcgacaugg ccagugacuc aagaugccca acacaagggg      840

aagcauaccu cgacaaacag ucagacaccc aauacgucug caagcgaaca cucguagacc      900

gcggcugggg caacggcugc ggacucuucg gcaagggcuc ucuggucaca ugcgccaaau      960

ucacguguag caagaaaaug acuggcaaau caauccaacc agaaaaucuc gaauaccgaa     1020

ucaugcuauc cguacacggc ucacaacaca gcggcaugau aguaaaugac auaggucaug     1080

aaacugacga aaacagagca aaagucgagg uaacccccaa cagcccgcgc gcugaagcaa     1140

cgcucggcgg cuucgggagc cuaggacucg acugcgaacc ccgaacgggc cuagacuuua     1200

gcgaucugua cuaccucaca augaacaaca aacacuggcu cgugcacaaa gagugguucc     1260

acgacauccc ccuccccugg cacgccggag ccgacaccgg cacaccccau uggaacaaca     1320

aagaagcccu cguggaguuc aaggacgcac acgcaaaacg gcaaaccgua gucguccuag     1380

gaucacagga aggcgccguc cacaccgccu uagcgggagc acucgaagca gaaauggacg     1440

gcgccaaagg gcgccuauuc uccggacacc ugaagugucg ucucaaaaug gacaaacucc     1500

gacuaaaagg aguguccuac agccucugca ccgcagcauu cacauucacc aaaguccccg     1560

ccgaaacccu ccacgggaca guaacagucg aaguccaaua cgccgguaca gacggaccau     1620

gcaaaguccc cgcccaaaug gcaguagaca ugcaaacacu aacccccguc gggcgacuca     1680

ucaccgccaa ccccguuaua acagaaagca cggaaaacag caaaaugaug cuggaacuug     1740

acccucccuu cggggacuca uacauaguaa uaggcgucgg agacaagaaa aucacacacc     1800

auuggcaccg aagcggaucc acaauaggaa aagcauucga agccaccgua cgaggggcaa     1860

aacggauggc aguacuaggc gacaccgccu gggacuuugg cucaguaggc ggcgucuuca     1920

acagccucgg gaaaggcauc caccaaauau ucggugccgc cuucaaauca cucuucggcg     1980

gaaugagcug guucagccaa auccugauug gaacccuacu cgucuggcug ggcuuaaaca     2040

ccaaaaacgg cagcaucucc cugacauguc ucgcccuagg gggaguaaug auauuccuau     2100

caaccgccgu cagcgcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7378
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 2 opt

<400>  7378
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcauagguau cauagggcuu cuacucacaa ccgccauggc agccgaaauc acccgacggg      120

gcuccgccua cuacauguac uuagacagaa gcgacgcagg aaaagccauc ucauucgcca      180

ccaaccuagg agucaacaag ugccacguac aaauaaugga ccugggacac augugcgacg      240

ccacuauguc cuacgaaugc cccaugcucg acgagggcgu cgaaccagac gacguugacu      300

gcuggugcaa caccacaagc acaugggucg ucuacgggac cugccaccac aagaaaggcg      360

aagcacgccg gagccggcgc gccgucacgc uuccgagcca cagcacccgc aaacuacaaa      420

caagaucaca gacguggcuc gagagcagag aauacaccaa gcaccucauc aaaguagaaa      480

acuggauuuu ccgcaacccc ggauucgcuc uagccgcagu cgcaaucgcc uggcuccuag      540

gcucgagcac aagccaaaaa gucaucuacc uaguaaugau ccuccuaauc gcacccgcau      600

acucaauacg cugcauaggc gucagcaacc gcgacuuugu agaaggaaug ucaggaggga      660

cauggguaga cguaguccuc gaacacggag gcugcgucac cgucauggca caggacaaac      720

ccaccguaga cauagagcuc guaaccacaa caguaagcaa uauggcggaa guccguuccu      780

acuguuacga ggcuucaauc ucagacaugg cgucagacuc acgauguccc acccaaggcg      840

aagcauaccu ggacaaacaa ucagacaccc aauacguaug uaaaaggacc cucguugacc      900

gcggcugggg gaacggaugc gggcuauucg gaaaaggauc ccucgucacc ugugccaaau      960

ucacaugcuc aaagaaaaug accggaaaau caauccagcc ggaaaaccua gaauacagga     1020

uaaugcucuc aguccacgga agccaacaca gcggaaugau agucaacgac gaaaacaggg     1080

ccaaagucga gguuacaccc aacagcccac gcgccgaagc aacacuagga ggauucggga     1140

gccuaggucu ugacugcgaa ccgcgaaccg gccuggacuu cucagaccuc uacuaccuaa     1200

ccaugaacaa caaacacugg cucguacaca aagaaugguu ccacgacauc ccgcucccgu     1260

ggcacgccgg agcagacaca ggcaccccac acuggaacaa caaagaggcc cucguagagu     1320

ucaaagacgc ccacgcgaaa cgccaaacag uaguaguacu ggguucccaa gaaggcgcag     1380

uccacaccgc gcuagcaggc gcacuagaag ccgaaaugga cggagccaaa ggacgucuau     1440

ucuccggaca ccucaaaugc cggcuaaaaa uggacaaacu aagacucaaa ggaguaucgu     1500

acucccucug cacagccgcc uuuacauuca cgaagguccc agccgaaacc cuacacggaa     1560

ccgugaccgu cgaaguacaa uaugcgggaa ccgacggccc augcaaggua ccagcccaaa     1620

uggcagucga caugcaaacc cucacgcccg ucggacgccu caucacagca aauccaguaa     1680

ucacagaaag caccgaaaau ucaaaaauga ugcuggaacu agacccaccc uucggagaca     1740

gcuacaucgu aaucggcguc ggagacaaga aaaucacaca ucacuggcau aggagcggga     1800

gcacaauugg aaaagccuuc gaagcaaccg uaagaggcgc caaacgaaug gcgguccuag     1860

gugacacagc cugggacuuc ggcagcgucg ggggcgucuu caacagccua ggcaaaggca     1920

uccaccaaau auucggagca gccuuuaaaa gccuauucgg uggcauguca ugguucagcc     1980

agauccucau aggaacccug cuagucuggc uaggacuaaa cacgaaaaac gggagcauaa     2040

gccugacaug ccucgcucug ggaggcguca ugauauucuu aagcacggcc guaagcgcau     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  7379
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 2 opt

<400>  7379
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcauaggaau aguaggccuc cuacucacaa ccgccauggc ggcggagauc accagacgag      120

gcagcgcaua cuacauguac cucgacaggu cagacgccgg gaaagcaauc agcuucgcaa      180

ccacacuggg agucaacaaa ugccacguac agauaaugga ccugggacac augugugacg      240

caaccauguc auacgagugc cccaugcuag augaaggagu agagcccgac gacguagacu      300

gcuggugcaa uacaaccagc acaugggucg uguacggaac cugccaccac aagaaaggcg      360

aagcuagaag aagccgcaga gcgguaaccc uaccaagcca cagcacgcga aaacuccaaa      420

cccgaagcca aaccuggcuu gagagccggg aauacacgaa acaccuaauc aagguagaaa      480

acuggauauu ccgcaauccc gguuucgcgc ucgucgcagu cgcaauagca uggcuccuag      540

gaagcagcac aagccagaaa gugauauacc uaguaaugau ccuacugaua gccccagcgu      600

acagcauccg cugcaucggc guaagcaacc gcgacuucgu agaaggaaug uccggaggca      660

cauggguaga cguaguccuc gagcacggcg gcuguguaac aguaauggca caagacaaac      720

caacggucga cauagagcua gucaccacaa caguaagcaa cauggcagaa gugcgaagcu      780

acugcuacga agcauccauc ucagacaugg ccagcgacag ucgaugcccc acacaaggcg      840

aagcauaccu agacaaacaa uccgacaccc aauacgucug caaaagaacg cuagucgaua      900

gagguugggg caacggcugc ggauuauucg gcaaaggcuc gcuagugaca ugcgcgaaau      960

ucacaugcag caagaaaaug acgggcaaaa gcauccagcc cgaaaaccuc gaguacagaa     1020

uaaugcuauc cguccacggc agccaacaca gcggcaugau agucaacgac acugggcacg     1080

aaacagacga aaacagggcc aaaguagaag ucaccccaaa cagcccacgc gcggaagcga     1140

cccucggagg guucggguca cucggacuag acugcgaacc acgaacaggg cucgacuucu     1200

ccgaccucua cuaccuaacg augaacaaca aacacuggcu gguacacaaa gagugguucc     1260

acgacauacc cuugccaugg cacgcagggg cggacacagg aaccccacac uggaacaaua     1320

aagaggcgcu agucgaguuc aaagacgcac acgcaaaacg acaaacagua guaguacuag     1380

gaagccaaga aggcgcagua cacacagcac uagccggcgc gcuggaagcc gaaauggacg     1440

gcgcaaaagg acgacuauuc agcggccacc ugaaaugcag gcucaaaaug gacaaacuga     1500

gacugaaagg agucuccuac ucacuaugca cagcagcauu uaccuucacg aaaguaccag     1560

ccgaaacccu acacggaaca guaacgguag aaguccagua cgcaggaaca gacgggccau     1620

gcaaaguacc agcacagaug gccgucgaca ugcagacccu cacaccaguc gggcgccuaa     1680

uuacagccaa cccgguaauc acagaaagca ccgaaaacuc caagaugaug cuagaacuag     1740

acccacccuu cggcgacagc uacaucguga ucgguguagg ugacaagaaa aucacacacc     1800

acuggcaccg gagcggcagc acaauaggga aagccuucga agcgacagua cgcggcgcga     1860

aacgcauggc cguacucggc gacaccgcau gggacuucgg aagcgugggc ggcgucuuca     1920

acagccucgg aaaagguauc caccaaaucu ucggggcagc cuucaaaagc cucuucgggg     1980

gaaugagcug guucucacaa auacuuauag ggacacugcu aguauggcuc ggccucaaca     2040

ccaaaaacgg aagcauaagc cuaaccugcc uagcacuggg aggcgugaug auauucuuaa     2100

gcacagccgu cucggcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7380
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 2 opt

<400>  7380
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gcauaggaau cgucggacua cuacuaacca ccgcaauggc cgcagaaauc acacgcagag      120

gaagcgcaua cuacauguac cuagauagga gcgaugccgg caaagccauc agcuucgcga      180

caacacuagg ugucaacaaa ugccacguac aaauaaugga ccuaggccau augugcgacg      240

ccaccauguc auacgaaugc cccaugcuag acgaaggcgu cgaaccagac gacguggacu      300

gcugguguaa uaccacaucc accuggguag ucuacggcac augccagcac aagaaaggag      360

aggcacgacg cucaagacga gcgguaacac uacccagcca cucaacccgc aaacugcaaa      420

cgcgcucaca aacauggcua gaaucaaggg aauacacaaa acaccuaaua aaagucgaaa      480

acuggauauu ccgcaacccc ggcuucgcac uggucgcagu agcaauagcg uggcuccuag      540

gcucgaguac cucccaaaaa gucauauacc ucgucaugau ccuccucaua gccccagcau      600

acuccauccg cugcaucggc gucagcaaca gagacuucgu agaaggcaug ucuggaggca      660

caugggugga cguggugcua gaacacggag gcugcgucac cguaauggcc caagacaaac      720

ccacagucga caucgaacuc gucacaacca cgguuagcaa cauggcagag guacgcuccu      780

acugcuauga agccagcaua ucagacaugg caagcgacuc gcgcugcccc acccaaggcg      840

aagccuaccu cgacaaacaa agugacacgc aauacguaug caaaagaacc cuagucgacc      900

gaggcugggg aaacgguugc ggccucuucg gaaaaggcag ccuaguaaca ugcgccaaau      960

ucacaugcuc caagaaaaug acagggaaaa gcauacaacc cgaaaaccug gaauacagaa     1020

ucaugcuuag cguacacggc agccaacauu cagguaugau aguaaacgac aucggccacg     1080

aaaccgauga gaaccgcgcc aaaguagaag ucacccccaa uuccccgcgc gccgaagcaa     1140

cccuaggcgg auucggauca cucggccuug acugcgaacc ccgcacagga cucgacuuca     1200

gcgaccucua cuaccuaaca augaacaaca aacacuggcu cguacacaaa gagugguuuc     1260

acgacauccc ccuacccugg cacgccggag ccgacaccgg aacaccccac uggaacaaca     1320

aagaagcccu aguggaguuc aaagacgcac acgccaaacg ccaaaccgua gucguccuag     1380

gcagccaaga aggcgccguc cacacugcgu uagcaggagc auuggaagcc gaaauggacg     1440

gggccaaagg ucggcuauuc agcggccacc ugaaaugccg ccucaaaaug gacaaacuuc     1500

gccuaaaagg cgucuccuau ucacucugca ccgccguaug cacagcagcc aaaguaccgg     1560

cggaaacccu acacggcacc guaacggucg aaguacaaua cgcaggaaca gacgguccau     1620

gcaaaguacc cgcucaaaug gccguagaca ugcaaacccu cacccccgua ggcagacuaa     1680

ucaccgccaa cccaguaaua accgaaagca cggaaaacuc caaaaugaug cuagaacuag     1740

acccgcccuu cggcgacagc uacaucguca uuggaguagg ggacaagaaa aucacacacc     1800

acuggcacag auccggaucc acaauaggca aagcguucga agcaaccguc cgcggcgcaa     1860

aacgcauggc cguccucggc gacacggcgu gggacuucgg uucaguaggc ggagucuuca     1920

acucccuagg caaaggaaua caccaaaucu ucggcgccgc auucaaaucg cuguucggcg     1980

gcaugucgug guucucacaa auacucaucg ggacccuccu aguauggcug ggacucaaua     2040

ccaaaaacgg aucaaucuca cucacaugcc uggcgcuggg cggcguaaug auauuccuau     2100

caacagcagu cucggccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7381
<211>  2431
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 2 opt

<400>  7381
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacua       60

gcaucggcau cgucggccuc cuacugacca cagcuauggc agcagaaauc acccgacggg      120

gguccgcaua cuacauguac cuagaccgcu cagacgccgg aaaagcgauc uccuucgcaa      180

caacgcuagg agucaacaaa ugccacgucc aaaucaugga ccuaggccac augugcgacg      240

cuacaaugag cuacgaaugc cccaugcuag augagggcgu agaacccgac gacgucgacu      300

gcuggugcaa caccacuagc acguggguag uauauggcac augccaccac aaaaagggag      360

aagcacggcg gagccgacgc gcaguaacac ucccaagcca cagcacacga aaacuccaaa      420

cccguagcca aacguggcuc gaauccagag aauacaccaa acaccuaauc aaaguagaaa      480

acuggaucuu ccgaaaccca ggcuucgccc ucguagcagu cgcaauagca uggcuccuag      540

gauccaguac aagccagaaa guaaucuacc uaguaaugau ccuccuaaua gcacccgccu      600

acuccaucag augcauaggc guaagcaacc gagacuuugu ggaaggaaug uccggcggaa      660

cauggguaga cgucgugcug gaacacggag gcugcguaac gguaauggcg caagacaaac      720

ccaccguaga caucgaacug gucaccacca ccguaucaaa cauggcagaa guacgcuccu      780

acugcuacga agcgucaaua ucagacaugg ccucggacuc ccggugccca acccaaggcg      840

aggcauaccu agacaaacaa uccgacaccc aauacgucug caaacgaacc cugguugauc      900

gaggaugggg uaacggcugc ggacucuucg guaaaggcag ucuagucacc ugugcaaaau      960

ucacaugcuc aaagaaaaug accggaaaau caauucaacc agaaaaccuc gaauaucgaa     1020

uaaugcucuc aguacacgga agccaacacu caggcaugau agucaaugac gaaaaccgcg     1080

ccaaaguuga agucacccca aacaguccca gagcagaagc cacgcucggc ggguucggga     1140

gccuaggacu ggacugugaa ccccggacgg gccuggacuu cagcgaccua uacuaccuca     1200

ccaugaacaa caaacacugg cuaguacaca aagaaugguu ucacgauaua ccccucccgu     1260

ggcacgccgg cgccgauacc ggcacucccc acuggaacaa caaagaagca cuggucgagu     1320

ucaaagacgc acacgcaaaa cgccaaacug ucguaguccu cgggucccaa gaaggcgccg     1380

uacacacagc acucgccggc gcgcucgagg cagaaaugga cggcgccaaa gguagacucu     1440

ucucaggaca ccuaaagugc cgccuuaaaa uggacaaacu ccgccucaaa ggagugucgu     1500

acuccuuaug caccgccgca uucaccuuca caaaaguacc agccgagaca cuccacggca     1560

ccguaaccgu agaaguacaa uacgccggga ccgacggacc augcaaggua cccgcacaaa     1620

uggccguaga caugcaaaca cucacgccag ucggacgccu caucaccgca aaucccguca     1680

ucacagaguc caccgagaac uccaaaauga ugcucgaacu cgauccccca uucggcgacu     1740

ccuacauagu caucggcguc ggagacaaga aaaucaccca ccacuggcac cgauccggcu     1800

cgaccaucgg caaagccuuc gaagccaccg uaagaggcgc caaacggaug gccguccugg     1860

gcgacacggc cugggacuuc ggauccgucg guggcguuuu caacucacuc ggaaaaggaa     1920

uccaccaaau cuucggagcc gccuucaaau cacuauucgg aggcaugucc ugguucagcc     1980

aaauccucau cggaacccua cucgucuggc uuggucucaa caccaaaaac ggcagcauca     2040

gcuuaacaug ccucgcacuc ggaggcguaa ugaucuuucu cuccaccgcc gucagcgcau     2100

gaggacuagu gcaucacauu uaaaagcauc ucagccuacc augagaauaa gagaaagaaa     2160

augaagauca auagcuuauu caucucuuuu ucuuuuucgu ugguguaaag ccaacacccu     2220

gucuaaaaaa cauaaauuuc uuuaaucauu uugccucuuu ucucugugcu ucaauuaaua     2280

aaaaauggaa agaaccuaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2400

cccaaaggcu cuuuucagag ccaccagaau u                                    2431


<210>  7382
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 2 opt

<400>  7382
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcauuggcau uauaggccua cuucucacca cugccauggc cgccgaaaua acacgacgag      120

gaagcgccua cuacauguac cuagacagau cggacgcagg caaagcaauc agcuucgcaa      180

caacccucgg agucaacaaa ugccacguac aaaucaugga ccuaggacau augugcgacg      240

caacaauguc auacgaaugc cccaugcugg acgaaggcgu cgaacccgac gacguagacu      300

gcuggugcaa caccacaagu accuggguag ucuacggaac cugccaccac aaaaagggag      360

aaacccgacg gucccgcaga ucaguauccc uccgcuacca cuacacaaga aaacuccaaa      420

cccgaagcca aaccuggcuc gaaucccgcg aauacaaaaa acaccuaauc augguagaaa      480

acuggauauu ccggaaccca ggauucgcca ucgugagugu agccaucaca uggcucaugg      540

gcagucugac cagccaaaaa gucaucuacc ucguaaugau cgugcuaaua guacccgccu      600

acagcaucuc augcaucggc gucucaaacc gcgaccucgu agaaggaaug uccggaggca      660

ccugggucga cgugguucuc gaacauggag gaugcgugac ggaaauggcc caagacaaac      720

caaccgucga caucgaacuc guaacgauga ccgucagcaa cauggcagaa gucagauccu      780

acugcuauga agcgucccuc agcgacaugg caagcgccag ccgaugcccc acacagggcg      840

agcccucacu cgacaaacaa ucagacacac aaagcgucug uaaaagaacc cucggggacc      900

gaggcugggg caacgggugc ggcauauucg gaaaagguuc gcuaguaaca ugcucaaaau      960

ucaccugcug caaaaaaaug cccggcaaau cgauacaacc agagaaccuc gaauacagaa     1020

uaaugcuacc cguacacggg agccaacaca gcggcaugau aguaaacgac auaggacacg     1080

agacagacga gaacagagca aaaguagaag ucacgccaaa uuccccgcga gccgaagcaa     1140

cacucggagg cuucggaagc cuagggcucg acugcgaacc acgcaccggc cucgacuucu     1200

cagaccuaua cuaccugacc augaacaaca aacacuggcu uguccacaaa gaaugguucc     1260

acgauauccc ucuacccugg cacgccggag cagacaccgg cacaccccac uggaacaaca     1320

aagaggcccu gguagaguuc aaagacgcac acgcaaaacg ucaaaccguc guggucuuag     1380

gcagccaaga aggcgccgua cacaccgcac ucgccggcgc ccucgaagcg gagauggacg     1440

gggcuaaagg acgccuguuc ucaggccauc ucaaaugccg ucuaaaaaug gacaaacuaa     1500

gacuaaaagg aguaucguac agccucugca ccgccgccuu cacauucacc aaaguaccgg     1560

cagaaacacu gcacggaaca gugacagugg aaguccaaag cgccggaaca gauggacccu     1620

gcaaaguccc cgcccagaug gccguagaca ugcaaacacu cacacccgua ggccggcuaa     1680

ucacagcaaa cccgguaauc accgagucaa cagaaaacuc caaaaugaug cuggaacugg     1740

auccgccauu cggagacuca uacauaguca uagguguagg agacaagaaa auaacccacc     1800

acuggcaccg aagcggcagc accauaggaa aagccuucga agccacggua cgcggagcca     1860

aaagaauggc cgugcucgga gacaccgccu gggacuucgg cucaguaggg ggcguauuca     1920

acagccuagg aaaaggcaua caccaaaucu ucggagcagc auuuaaaagc cuauucggag     1980

gaaugagcug guucucacaa auacuaaucg gaacccuacu aguguggcua ggacucaaca     2040

ccaaaaacgg cagcaucuca cucacgugcc uagcccuggg aggagucaug auauuccucu     2100

ccacagccgu auccgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7383
<211>  2446
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 2 opt

<400>  7383
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcg       60

uaggcauagu cggccuacuc cugacgaccg caauggccgc agaagugaca cggcggggca      120

gcgccuacua cauguaucug gacagaaacg acgcggggga agccaucuca uucccaacga      180

cccuaggcau gaacaaaugc uacauacaaa uaauggaccu agggcacaug ugcgacgcaa      240

cgauguccua cgagugcccg augcucgacg aagguguaga accagacgac gucgauugcu      300

ggugcaacac cacauccaca uggguaguau acggcacuug ccaccauaag aaaggggaag      360

cacgccgcag ccgacgcgcc guaacacucc cuagccacuc cacgcgcaaa cuacaaacaa      420

gaagccaaac cuggcuagaa ucuagagaau acacgaaaca ccugauaaga guagaaaacu      480

ggaucuuccg aaacccggga uucgcccucg ccgcagccgc cauagcaugg cuccucggaa      540

gcaguaccag ucaaaaagua aucuaccugg uaaugauccu ccuaaucgca cccgcguaca      600

gcaucaggug caucggcgug agcaaccgcg acuucguaga agggaugagc ggcggaacau      660

gggucgacgu cguccuagag cacggaggau gcgucacagu aauggcacaa gacaaaccua      720

cgguagacau cgagcucguc accaccaccg uaaguaacau ggccgaagua cggucauauu      780

gcuacgaagc auccauaagc gacauggcca gcgacucccg auguccaacu caaggagaag      840

cauaccuaga caaacaaucg gacacacaau acguaugcaa aagaacccuc guagacagag      900

gauggggcaa cggaugcgga cuauucggaa aaggcucgcu aguaaccugc gccaaauucg      960

cgugcuccaa gaaaaugaca ggaaaaagca uucaaccaga aaaccuagaa uacagaauaa     1020

ugcuguccgu acacggaagc caacauagcg gaaugaucgu caacgacacc ggacacgaaa     1080

cggacgagaa cagagcaaaa guagaaauca ccccaaacag uccccgcgca gaggcgacgc     1140

uaggagguuu cggaagccua ggacuggacu gcgaaccccg cacgggccuc gacuucagcg     1200

accuguacua ccucaccaug aacaacaaac acuggcuagu gcacaaagaa ugguuucacg     1260

acaucccccu accguggcac gcaggcgcag acacaggaac accgcacugg aacaacaaag     1320

aagcacuggu cgaguucaaa gacgcccacg caaaaagaca gacaguggug guccugggca     1380

gccaagaggg agccguacac acagcgcucg cgggagcacu ggaagccgag auggacggcg     1440

caaaaggacg ccuguccuca ggacaccuca aaugccgacu aaaaauggac aaacucagac     1500

ucaaaggagu cagcuacuca cuuuguacag ccgccuucac auucacaaaa auaccagccg     1560

aaacacuaca cggaacagua accgucgaag uacaguacgc aggaacagac ggaccgugca     1620

aaguccccgc gcaaauggca guagacaugc aaacccugac cccagucgga cgacuaauaa     1680

ccgcaaaccc cguaaucaca gaauccaccg aaaauuccaa aaugaugcuc gagcuagacc     1740

cacccuucgg cgacagcuac auagugaucg gcgucgggga aaagaaaaua acccaccacu     1800

ggcaccgaag ugggaguaca auaggcaaag cauucgaggc aaccgucaga ggggccaaac     1860

gaauggcagu acugggagac acagcauggg acuucggauc agugggcgga gcccuaaacu     1920

cacucggaaa aggaauccau caaaucuucg gggccgcguu caaaucacuc uucgguggca     1980

ugagcugguu cagccaaaua cucauaggca cacuccuaau guggcucgga cugaacacga     2040

aaaacggaag cauaagccug augugccugg cacuaggcgg cguccucaua uuuuuaagua     2100

cagccguaag cgccugagga cuagugcauc acauuuaaaa gcaucucagc cuaccaugag     2160

aauaagagaa agaaaaugaa gaucaauagc uuauucaucu cuuuuucuuu uucguuggug     2220

uaaagccaac acccugucua aaaaacauaa auuucuuuaa ucauuuugcc ucuuuucucu     2280

gugcuucaau uaauaaaaaa uggaaagaac cuagaucuaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2400

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2446


<210>  7384
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 2 opt

<400>  7384
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguggggau agucggacua cuccugacaa ccgccauggc cgccgaaguu acccgacgag      120

gcagcgccua cuacauguac cucgaccgca acgacgccgg cgaggcaauc uccuucccca      180

ccacgcuagg caugaacaaa ugcuacauac aaaucaugga ccucggacac augugcgacg      240

caacaaugag cuacgaaugc cccaugcucg acgagggagu cgaacccgac gaugucgacu      300

gcuggugcaa cacaaccagc accugggucg uguacggaac cugccaccac aaaaagggcg      360

aagcgcgccg cucccggcgc gccgucaccc uccccagcca cucaacacga aaacuacaaa      420

cacgaagcca aacauggcug gaaucacgag aauacaccaa acaccuaaua cgagucgaaa      480

acuggauauu ccgaaacccc ggcuucgcac uagccgccgc ggccauagcc uggcuccucg      540

gcucaucaac cucccaaaaa guaaucuacc ucguaaugau acuccuaaua gcccccgcau      600

acucgauaag augcauaggc gucagcaacc gcgacuucgu agaaggaaug agcggcggca      660

ccugggucga cguuguccua gaacacggcg gaugcgucac aguaauggcc caagacaaac      720

cuaccgucga cauagaacuc gucacaacaa ccguuuccaa cauggccgaa guacgaucau      780

acugcuacga ggcuucaaua uccgacaugg ccucggacuc ccgcugcccc acccaaggcg      840

aagccuaccu cgacaaacag agcgacaccc aauacgucug caaacgcacg cuagucgacc      900

gcggaugggg caacggcugc ggacuauucg ggaaaggauc ccuaguaaca ugugcaaaau      960

ucgccugcuc aaagaaaaug acggguaaau caauccaacc ggaaaaccuc gaguaucgaa     1020

ucaugcucuc cguccauggc ucccaacacu ccggcaugau aguaaaugac acaggccacg     1080

aaaccgauga aaacagagcu aaaguugaaa uaacccccaa cucaccacgc gccgaagcca     1140

cccugggcgg cuuuggauca cugggccucg acugcgaacc ccgaaccggu cucgacuuca     1200

gcgaccugua cuaccuaaca augaacaaca aacacuggcu aguccacaag gagugguucc     1260

acgacauccc acucccaugg cacgccggag cagacacagg caccccacac uggaacaaca     1320

aggaagcccu agucgaguuc aaagacgcac acgcaaaacg acaaacgguc gucguccuag     1380

ggagccaaga aggagcagua cacacagcac ucgcaggggc gcuagaagca gaaauggacg     1440

gggcaaaagg acgccuuucc agugggcacu uaaagugcag acucaaaaug gacaaacuua     1500

gauuaaaagg cguaucauac ucacuaugca ccgccgccuu caccuucacc aaaauacccg     1560

ccgaaacgcu gcacggaaca gucacgguug aaguccaaua cgccggcaca gacgggcccu     1620

gcaaaguacc cgcgcaaaug gcggucgaca ugcagacacu uacccccguu ggucggcuaa     1680

uaacggccaa cccagucaua acagaaagca ccgaaaacuc caaaaugaug cuagaacucg     1740

acccacccuu cggagacagc uacaucguca ucggcguugg cgaaaagaaa aucacacauc     1800

acuggcacag aucagguagc accauaggca aagccuuuga agccacaguc agaggcgcca     1860

ggcguauggc aguccucgga gacaccgccu gggacuuugg auccguggga ggcgcacuaa     1920

acucccuagg caaaggcauc caucaaauau ucggggccgc cuucaaaucg cuguucggcg     1980

gaaugucaug guucagccaa auccuaaucg gaacgcuccu uauguggcuc ggccuaaaca     2040

ccaaaaacgg auccauaucc cucaugugcc uggcccucgg aggcguccua aucuuccugu     2100

ccacagcggu cuccgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7385
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 2 opt

<400>  7385
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccguuggcau cgugggccuc cuccuaacaa ccgcaauggc cgccgaagua acccgacgug      120

gaagugcaua cuacauguac cuagaccgca acgacgcagg agaagcaauc ucauucccaa      180

ccacccuagg caugaacaaa ugcuacaucc aaaucaugga ccuaggccac augugcgacg      240

caaccauguc auacgaaugc cccaugcuag acgaaggugu ugaaccagac gacguagacu      300

gcuggugcaa cacaacaucc accuggguag ucuacggcac augccaccac aaaaagggag      360

aagcacggcg cucccgccga gccgucaccc ugcccaguca cuccacccgc aaacuccaaa      420

cccgauccca aaccuggcua gaaagccgcg aauacacaaa acaccuaaua agaguagaaa      480

acuggauguu cagaaacccc ggauucgccc ucgccgcagc agccauagca uggcuccucg      540

gcucauccac cucacaaaaa guuauauacc ucgucaugau ccuacuaaua gccccagccu      600

auagcaucag augcaucggc gucuccaacc gagacuucgu cgaaggaaug ucaggaggaa      660

ccugggugga cgucgugcuc gaacacgggg gcugcgugac cguaauggca caagacaaac      720

caacgguaga caucgagcua gucacaacaa cuguguccaa cauggccgaa guacgaagcu      780

auugcuacga agcauccauc ucggacaugg ccagcgacuc ucgaugcccc acccaaggcg      840

aagcauaccu ggacaaacaa uccgacaccc aauacguaug caaacguacu cuaguagacc      900

gcggcugggg gaacgguugc ggacuguucg gaaaaggguc acugguaacc ugcgcaaagu      960

ucgcgugcuc uaagaaaaug accggaaaau ccauccaacc agaaaaccua gaauaccgca     1020

ucaugcuauc cguccacgga ucccagcaca guggaaugau ugucaacgac accggacaug     1080

aaacagacga aaaccgcgcc aaaguagaaa uaacaccaaa cucaccccgc gccgaagcaa     1140

cacucggcgg auucggcuca cucggacucg acugcgaacc ccgcaccggc cucgacuucu     1200

cagaccugua cuaccucaca augaacaaca aacacuggcu cguacacaaa gaaugguucc     1260

acgacauacc acucccuugg cacgcgggcg ccgacacagg uaccccacac uggaacaaca     1320

aagaagcccu agucgaguuc aaagacgcac acgccaaaag acagacggua gucgugcucg     1380

gaagccagga aggagccgua cacaccgccc ucgccggggc acuggaagca gaaauggacg     1440

gcgcuaaagg ccgccucuca uccggccacc uaaaaugccg guuaaaaaug gacaaacuga     1500

gacucaaagg aguaagcuac uccuuaugca ccgccgccuu caccuucacc aaaauaccgg     1560

ccgaaacccu ccacgguacc guaacaguag aaguccaaua cgccggaaca gacggccccu     1620

gcaaaguccc ugcccagaug gcugucgaca ugcaaacccu cacccccguc ggaagacuaa     1680

ucacugccaa ccccgucaua accgaaucca ccgaaaacag caaaaugaug uuggaacucg     1740

aucccccauu uggcgacuca uacaucguga uaggcguagg cgagaaaaag auaacacacc     1800

acuggcaccg uuccggaagu accaucggca aagcauucga agcgaccguc cgaggcgcaa     1860

aacgaauggc cguacucggg gacacggccu gggacuucgg gagcgugggc ggcgcacuaa     1920

acucccucgg caaaggaauc caccaaaucu ucggcgccgc cuucaagucc cucuucggag     1980

gcaugucgug guucucccag aucuuaaucg gaacucuacu cauguggcua ggguugaaca     2040

ccaaaaacgg aucaauaucc cuaaugugcu uagcccuugg aggaguacua aucuuccuaa     2100

gcaccgcagu gucagccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7386
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 2 opt

<400>  7386
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

guguaggcau agugggacua cuacucacua ccgccauggc agccgaagua acaaggcgag      120

gauccgcgua cuacauguac uuagaccgca acgacgcagg agaagcaauc uccuuuccca      180

ccacacucgg aaugaacaaa ugcuacaucc aaauaaugga ccuaggccac augugcgacg      240

ccacaauguc cuacgaaugc ccgaugcucg acgaaggagu cgagccagau gacguagacu      300

gcuggugcaa caugaccagc accugggucg ucuacggcac cugccaccac aaaaaaggag      360

aggcgcgaag auccagacgg gccgucacac uaccaaguca cuccacgcgu aaacuccaga      420

ccagaagcca aaccuggcuc gaaucgcgag aauacacaaa acaccuaauc cgcgucgaga      480

acuggauauu ccguaacccc ggauucgccc uagcagccgc cgcaaucgcc uggcuacuag      540

gcagcucaac cagccaaaaa guuaucuacc uugucaugau acuccucauu gcgccagcau      600

acucgaucag auguauuggu gucaguaacc gcgacuucgu agaaggcaug uccggcggaa      660

caugggucga cgucguucuc gaacacgggg gaugcgucac cgucauggca caagacaaac      720

caaccgucga cauagaacuc guaacuacaa ccgucuccaa cauggccgaa guccgcagcu      780

acugcuacga agccagcauc ucagacaugg cgagcgauuc ccgcugcccc acgcaaggcg      840

aagccuaccu agacaaacaa agcgacaccc aauacguaug uaaacggacc cucgucgaca      900

gaggaugggg caacggcugc ggccuuuucg gcaaaggcag ccuaguaacc ugcgccaaau      960

ucgccugcag caaaaaaaug accggaaaaa gcauucaacc ugaaaaccua gaguaccgca     1020

ucaugcucuc cguccacgga ucgcaacacu ccggcaugau agucaaugac accggacacg     1080

aaaccgacga aaaccgugcg aaaguggaaa ucaccccuaa uagccccagg gccgaagcca     1140

cacucggcgg auucggcagc cuagggcuag acugcgaacc cagaacaggc cuagacuuca     1200

gcgaccuaua cuaccucaca augaacaaca aacacuggcu aguccacaaa gaaugguucc     1260

acgacauccc acuaccaugg cacgcuggag ccgacacagg gacuccccac uggaacaaca     1320

aagaagcgcu cguagaguuc aaagaugcac acgcaaaacg gcaaacaguc guaguccuag     1380

gcucacagga aggcgcgguc cauacagccc ucgcaggagc acucgaagca gaaauggacg     1440

gagccaaagg ccgacuaagc agcgggcacc ucaaaugcag acucaaaaug gacaaacuca     1500

gacugaaagg cguaagcuac agccuuugca cagcagccuu caccuuuacc aaaauacccg     1560

ccgaaacccu acacggcacc guaacgguag aaguucaaua cgcaggcacc gacggaccau     1620

gcaagguccc cgcacaaaug gccgucgaua ugcagacccu aacccccguc ggacgccuca     1680

ucaccgccaa cccagucaua accgaaagca cagaaaacuc gaaaaugaug cucgaacuag     1740

acccacccuu cggagacuca uacaucguaa ucggagucgg agaaaaaaaa auaacacauc     1800

acuggcaccg auccggcuca acaauaggca aagccuucga agcaacagua cgaggagcga     1860

aacggauggc cguacucgga gacacagccu gggacuucgg cagcgucgga ggagcccuca     1920

acagcuuagg aaaaggcauc caccaaauau ucggagccgc auucaagucc cuguucggcg     1980

gcauguccug guucagccaa auacuaauag gcacgcuacu gauguggcuc ggacucaaca     2040

ccaaaaacgg cagcaucagc cucaugugcc ucgcgcucgg aggcguccuc auauuccuuu     2100

caaccgcagu cucagcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7387
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 2 opt

<400>  7387
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcgucgggau cgugggccuc cuacuaacga ccgcaauggc agcagagguc acccgcagag      120

gaagcgcaua cuacauguac cuagaccgaa acgacgccgg agaagcaauc agcuucccca      180

caacccucgg caugaacaaa ugcuacauac agaucaugga ccuaggacac augugcgacg      240

ccaccaugag cuacgaaugc ccgaugcuag acgaaggcgu cgaaccagac gacgucgacu      300

gcuggugcaa cacaacaagc acauggguag ucuacggaac cugccaccau aagaaaggcg      360

aagcgcgcag aagccggcga gcaguaaccc uaccaagcca cuccacaaga aaacuacaaa      420

ccagaucaca gaccuggcuc gaaagccggg aauacaccaa acaccucauc cgcgucgaaa      480

acuggaucuu cagaaauccc ggcuucgccc uagcggcagc cgccaucgcc uggcuauuag      540

gcagcaguac cucccagaaa guaauauacu uagucaugau acuacuaaua gcgccagcgu      600

acucgauacg augcauagga gucagcaacc gagacuucgu cgaaggcaug agcggaggga      660

cgugggugga cguaguacuc gaacacggag gcugcgucac ggcgauggcu caagacaaac      720

ccacagucga caucgagcua gucaccacaa ccguaagcaa cauggccgag guaagaucau      780

acugcuacga ggcauccaua agcgacaugg caagugacuc aagaugcccc acacaaggag      840

aagcauaccu cgacaaacag uccgacaccc aauacgucug uaaaagaacc cuaguagaca      900

gaggcugggg caacgggugc ggacuauucg gcaaaggcag ccucgugaca ugcgcgaaau      960

ucgcuugcuc caagaaaaug acaggcaaau ccauccaacc cgaaaaccuc gaauaccgca     1020

uaaugcuaag cguacacggg ucccaacacu ccggcaugcu ggugaacgau accggacacg     1080

agaccgacga aaacagagcg aaaguagaaa uaaccccaaa uagcccgcga gcagaagcaa     1140

cucuaggcgg auucggcagc cuuggacuag acugcgaacc aagaacaggc cucgacuucu     1200

cagaccuaua cuaccucacc augaacaaca aacacuggcu cgcgcacaaa gaaugguucc     1260

acgauauccc ccuccccugg cacgcaggcg cagcaacggg aacaccacac uggaacaaca     1320

aagaagcccu cgucgaguuc aaagacgcgc acgccaaaag gcaaaccguc guaguacuag     1380

gaucccaaga aggagccguc cacaccgcac ucgccggagc acuagaagcc gagauggacg     1440

gcgcaaaagg acgacucucc agcggccacc ucaaaugccg acucaaaaug gacaaacugc     1500

gccuuaaagg cgucucguac uccuuaugca cggccgccuu caccuuuaca aaaauacccg     1560

cagagaccgu agacggcacc gucaccguag aagggcagua cgggggaacc gauggaccuu     1620

gcaaaguacc agcccaaaug gcaguagaca ugcagacccu uacacccgua ggacgacuaa     1680

uaacagccaa ccccgucaua accgaaucca ccgaaaacag caaaaugaug cuagaacuag     1740

aucccccauu cggcgacuca uacaucguga ucggggucgg agaaaagaaa auaacccacc     1800

acuggcacag gagcgggagc acaaucggca aagccuucga agccaccgua cggggcgcca     1860

aacgaauggc cguucucggc gacacagcau gggacuucgg cucggucggc ggagcacuaa     1920

acagccucgg aaaaggaaua caccaaauaa uaggcgcggc cuucaaauca cuauucgggg     1980

gcaugagcug guucagccaa auacugauag ggacgcugcu gauguggcuc ggacugaaua     2040

cgaaaaacgg cuccaucagu cuaaugugcc uagcccuagg aggcguacug auauuccuca     2100

gcacagcagu auccggcuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  7388
<211>  2440
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 2 opt

<400>  7388
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggcg       60

ccgacgccag caucggcauc gucucccuac uccuaacaac cguaauggca gcagagauaa      120

cgcgacgagg auccgcguac uacauguacc uagacagaag cgacgccggc aaggccaucu      180

cauucgucac aacgcuaggc gucaacaaau gccacguuca aauaauggau cucggccaca      240

ugugugacgc uaccaugagc uacgaaugcc caaugcucga cgagggcguc gaacccgacg      300

acguggacug cuggugcaac accaccucaa caugggucgu auauggcacg ugccaccaca      360

agaaaggcga agcccgccga ucaaggagag ccgucacccu cccaagccac ucaaccagaa      420

aacuccaaac cagaagccag accuggcucg agagccgaga auacacaaaa caccugauaa      480

aaguagaaaa cuggaucuuc cgaaacccag gcuucgcgcu agucgcagua gccauagccu      540

ggcuccucgg cagcuccaca ucacagaagg uuauauaccu agucaugaua cuccucaucg      600

ccccagcaua cagcauccgc ugcauaggcg uaagcaaccg cgacuucguc gaggggaugu      660

ccggaggaac gugggucgac guagugcuag aacacggugg cugcguaacc gucauggcgc      720

aagacaaacc gaccgucgac aucgagcuag ucacaacgac aguaucaaac auggccgaag      780

uacgaagcua cugcuacgaa gccagcauca gcgacauggc cucagacagc agauguccaa      840

cccaaggaga agcauaccuc gacaaacaaa gcgacacgca auacgucugc aaacgcaccc      900

uaguagaccg uggauggggc aacggcugcg gccuguuugg aaaaggcuca cuggucacau      960

gugccaaauu uacaugcagc aagaaaauga cgggcaaaag cauacaacca gaaaaccuag     1020

aauaccgcau caugcuaagc guccacggaa gccaacacuc cggaaugaua gucaacgacg     1080

aaaaccgagc aaaagucgaa guaacaccca acucaccaag agcagaagca acacucggcg     1140

gcuucggaag ccugggccua gacugcgaac ccagaaccgg acuagacuuc agcgaccugu     1200

acuaccucac caugaacaac aaacacuggc ucguccacaa ggaaugguuc cacgacauac     1260

cacuaccaug gcacucagga gccgacaccg aaaccccaca cuggaacaac aaagaagcac     1320

ugguggaauu uaaagacgca cacgccaaaa gacagaccgu cguaguccuc gggagccaag     1380

aaggcgccgu ccauacagca cuggccggag cccuagaggc cgaaauggac ggagcaaaag     1440

gacgccuauc auccggccac cucaaaugcc gccucaaaau ggacaaacuc cgacuaaaag     1500

gcguauccua cucccugugc accgccgcau ucacauucac aaaaguacca gccgaaacac     1560

ugcacggcac ggugaccgua gaaguccaau acgcaggacg cgacggccca ugcaaggucc     1620

ccgcacaaau ggcaguagac augcagacau uaacgccagu cggaagacuc auaaccgcca     1680

accccgucau aacugaaagc accgaaaacu caaaaaugau gcuugaacua gacccacccu     1740

ucggagacuc cuauaucguc auaggaguag gcgacaagaa aauaacacac cacuggcaca     1800

gaucaggcag cauaaucggg aaagccuucg aggccacagu ccgcggcgcc aaacgcaugg     1860

caguccuagg ggacacagca ugggacuucg gcuccguggg cggaguuuuc aacucccuag     1920

gaaagggaau ccaccaaauc uucggcgccg ccuucaaauc acucuucgga ggaauguccu     1980

gguucucuca aauccuaauc ggaacccuac ucgucuggcu cggccuaaac accaagaacg     2040

gcagcauaag ccuaaccugc cucgcgcucg gaggcguaau gauauuccuc ucaacugcgg     2100

ucagcgcaug aggacuagug caucacauuu aaaagcaucu cagccuacca ugagaauaag     2160

agaaagaaaa ugaagaucaa uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc     2220

caacacccug ucuaaaaaac auaaauuucu uuaaucauuu ugccucuuuu cucugugcuu     2280

caauuaauaa aaaauggaaa gaaccuagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2400

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2440


<210>  7389
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 2 opt

<400>  7389
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggag       60

cagacaccag caucggaauc auaggacuac uccuaacaac agcuauggcg gccgaaauca      120

cgagaagagg aagcgcauac uauauguacc ucgaccguuc cgacgcaggc aaagcgauau      180

cauucgcaac cacaaugggc gugaacaaau gccacgucca aaucauggac cuaggucaca      240

ugugugacgc caccaugucu uacgaaugcc ccaugcucga cgaaggaguc gaacccgacg      300

acguagacug cuggugcaac accaccucca cauggguugu guacggaaca ugccaucaca      360

agaaaggaga agcccggccg ucccgccgcg caguaacgcu cccgagccac uccaccagaa      420

aacuacaaac ccgcucacaa acauggcucg aaagucgcga guacacaaaa caucucauaa      480

aagucgaaaa cuggauacua cgcaacccag gcuucacagu ggccgcgcuc gccauggccu      540

ggcuacuggg aaacaguacg agccagcgcg uaaucuaccu cguaaugauu cuccugaucg      600

cacccgcaua cuccauacgc ugcaucggcg uuagcaaccg cgacuucgua gaaggaaugu      660

ccggaggaac cuggguagac gugguccuag aacacggagg augcgucacc gucauggccc      720

aagacaaacc aacagucgac auagagcuag uaacaaccac ggucuccaac auggccgaag      780

ucagaagcua cugcuacgaa gcauccauca gcgacauggc aagcgacucc cgcuguccca      840

cacaagggga agccuaccuc gacaaacagu ccgacacaca auacgucugc aaacgaaccc      900

ucguggaucg cggauggggc aacggaugcg gacucuucgg aaaaggaagc cuagugaccu      960

gugcaaaguu cacgugcucc aagaaaauga caggaaaauc cauacaaccc gaaaaccucg     1020

aauaccggau caugcucagc guccacggau cccagcacag cggaaugaua gugaacgaca     1080

uaggccacga aaccgacgaa aaccgagcaa aaguggaagu caccccuaau ucaccccgcg     1140

cagaagcaac acugggcggc uucggcagcc ucggccuaga cugcgaaccg cgcaccggac     1200

uagacuuuuc agaccuguac uaucuaacaa ugaacaacaa acacuggcuu guccauaaag     1260

aaugguucca cgacaucccc cucccauggc augcaggcgc agacacaggc acaccccacu     1320

ggaacaacaa agaggcacuc guggaguuca aagacgcaca cgccaaaaga caaaccgucg     1380

uaguacucgg cucccaagaa ggagcagucc acaccgcucu ggcaggagca uuggaggcag     1440

aaauggacgg cgcgaagggc cgacucuucu caggacaccu caaaugccgg auaaaaaugg     1500

acaaacuaag gcuaaaaggc gucagcuacu cacucugcac cgccgcauuc acauucagca     1560

aagucccggc agaaacgcuc cacggaacag ucacggugga ggugcaauac gccggccgag     1620

acggacccug caaagucccc gcccaaaugg caguagacau gcaaacgcuc acccccguag     1680

gaagacuuau cacggccaac ccaauaauaa cagaauccac cgaaaacagc agaaugaugc     1740

ucgagcucga cccucccuuc ggcgacucau acauaguuau cggaguaggc gacaagaaaa     1800

ucacgcacca cuggcacagg ucaggcagca caaucggaaa agccuucgaa gccaccgucc     1860

gcggcgcaaa aagaauggcc guacucggcg acacagccug ggacuuugga agcgucggcg     1920

gagucuucaa cucacuaggg aaaggaguuc accaaguauu cggagccgcc uucaaaucgc     1980

uauucggcgg gauguccugg uucucgcaaa uccucaucgg aacccuucua gucuggcucg     2040

gacugaacac caaaaacgga agcaucuccc uagcaugccu agcccucggc ggugcaauga     2100

uauuccuaag cacggccgua agcgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  7390
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 2 opt

<400>  7390
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaggucau auaccugguc augaucuuac ucauagcccc ggcauacagc auaagaugua      120

uaggagucuc gaacagagac uucgucgaag gaaugagcgg aggcacgugg guggacauag      180

uccuggaaca cggaggaugc gugaccguga uggcgcaaga caaaccgacc guggacauag      240

aacugguaac aaccacgguc ucgaacaugg ccgaggucag gagcuacugc uacgaagcaa      300

gcauaagcga cauggcaagc gauaguagau gcccgacaca aggcgaggca uaucuagaca      360

aacaaagcga uacccaauac guauguaagc gcacauuagu ggaccgagga uggggaaacg      420

gcugcggacu guucgggaaa ggaagccuag ucacgugugc gaaguucgcg ugcagcaaga      480

aaaugaccgg caaaucgaua caacccgaga accuagaaua cagaauaaug cuaagcguac      540

acgggagcca gcacucgggc augaucguaa acgacaccgg acacgagacc gacgaaaacc      600

gagcgaaagu ggaaauaaca cccaacucgc cccgagcaga ggccacccua ggaggauucg      660

gcucgcucgg gcuagacugc gagcccagga cuggucuaga cuucucagac uuguacuacc      720

ucacgaugaa caacaagcac uggcuaguac acaaggaaug guuccacgac auaccacugc      780

ccuggcacgc gggagccgac acagguacac cccacuggaa uaacaaggag gcccucgugg      840

aguucaaaga cgcccacgca aaacgccaaa ccgugguagu acucggcagc caggaagggg      900

ccguccacac ggcccuggca ggggcacugg aagcggaaau ggacggggca aagggcagac      960

uaucgagcgg acaccuaaaa ugucgccuaa aaauggacaa gcuaaggcuc aagggcguaa     1020

guuacagccu augcacggcc gcauucacgu ucacgaaaau acccgcagaa acgcuacacg     1080

gcacggucac ggucgagguc caauacgcgg gcacagacgg acccugcaaa gugcccgcgc     1140

aaauggccgu ggacaugcaa acgcucacgc ccgucggacg gcucauaacc gcaaacccgg     1200

uaauaaccga aaguacggaa aacagcaaaa ugaugcucga guuagaccca ccauucgggg     1260

acucauacau aguaauaggc gugggcgaga aaaagauaac gcaccacugg caccgcucgg     1320

gaucuaccau cggcaaggcu uuugaagcaa caguacgcgg ggcaaaacgc auggccgugc     1380

ucggggacac ggccugggac uuuggcucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7391
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 2 opt

<400>  7391
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaagucau cuaccuaguc augauccuac ucauagcccc cgccuacagc auccgcugca      120

ucggagucuc aaaccgcgac uucguugaag gcaugucagg aggcacaugg gucgacgugg      180

uacuagaaca cggcgggugc gucacaguca uggcccaaga caaacccaca guggacaucg      240

aacuggucac caccacagua ucaaacaugg cagaaguuag aagcuacugc uacgaggcaa      300

gcauauccga cauggccagc gacucaagau gcccaacaca aggcgaagcc uaccuggaca      360

aacaaagcga cacucaauac guaugcaaac gaacgcuagu ggacagagga uggggaaacg      420

gauguggccu cuucggcaaa ggcagccucg ucacaugcgc aaaauucgcc ugcucaaaga      480

aaaugaccgg aaaauccauu caacccgaga accuagaaua cagaaucaug cuauccgugc      540

acggcagcca acacuccgga augauaguca acgacaccgg ccacgaaacc gacgaaaacc      600

gcgcaaaagu ggaaauaacc ccaaacucac ccagagccga agcaacacua ggcggcuucg      660

gcucccucgg acuagacugc gaacccagaa ccggccucga cuucucagac cuauacuauc      720

uaaccaugaa caacaaacac uggcucgugc acaaagaaug guuccacgac auaccccuac      780

ccuggcacgc aggggccgau acaggcacac cccacuggaa uaacaaagaa gcccucguag      840

aguucaaaga ugcacaugca aaacgacaga ccguagucgu gcucggcagu caggaaggcg      900

ccguccacac ggcccuggcc ggagcccucg aagcagaaau ggacggcgca aaaggccgac      960

ucuccagcgg acaccuaaaa ugccgccuaa aaauggacaa acuacgccuc aaaggcguca     1020

gcuacagccu cugcacagca gcauucacgu ucacaaaaau accagcagaa acacuccacg     1080

gcacaguaac agucgaaguc caguacgccg gcacagacgg acccugcaaa guaccagcac     1140

aaauggcagu agacaugcaa acgcucacgc ccguaggacg ccuaaucacc gcaaauccag     1200

ucaucaccga aagcaccgaa aacagcaaaa ugaugcucga acucgauccc ccauucggcg     1260

acucguacau agucaucggc gugggagaaa agaaaaucac acaccacugg caccgcuccg     1320

gaagcaccau aggcaaagcc uucgaagcca ccguacgcgg agccaaacgc auggcaguac     1380

ucggagacac agccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7392
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 2 opt

<400>  7392
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguaau auaccuaguc augauccuac ucaucgcccc agcuuacagc auacgcugca      120

ucggggucuc aaacagggac uucgucgaag gcaugagcgg aggaacaugg guggacgugg      180

uacuagaaca cggcggaugc guaaccguaa uggcgcaaga caagccuacc guagacaucg      240

aacuaguaac caccacaguc uccaacaugg ccgaagucag gaguuacugc uacgaagcaa      300

gcauaagcga cauggcgagc gacagccggu gcccgacaca aggugaagca uaccuagaca      360

aacaaaguga cacccaauac gucugcaaaa ggacccuagu agacaggggc uggggcaacg      420

gcugcggucu auucggaaaa ggcagccuag ucacaugcgc caaauucacc ugcagcaaaa      480

aaaugaccgg caaaucaauc caacccgaaa accucgaaua ccgcauaaug cugagcgugc      540

acgggucaca acacucggga augaucggau acgaaacaga cgaagaccgc gcaaaaguag      600

aaguaacacc gaacucgcca agagcagaag ccacccucgg aggauucggc ucucucggac      660

ucgacuguga accaagaacc gggcuagacu ucucagaccu cuacuaccua acgaugaaca      720

acaaacacug gcucgugcac aaagaauggu uccacgacau accccugccc uggcacgcag      780

gcgcagacac gggcacccca cacuggaaca acaaggaagc acuaguagaa uucaaagacg      840

cccacgccaa gcgucaaaca guagucgugc ucgggagcca agaaggggcc guacacacgg      900

cccuagcagg ggcccuagaa gcagaaaugg acggggcaaa aggaagacua uucuccggac      960

accugaaaug ccgccuaaaa auggacaaac ucagacuaaa gggcguaagc uacagccuau     1020

gcacagccgc auucacauuc acaaaagucc ccgcagaaac acuacacggc accgucaccg     1080

ucgaagucca auacgcgggc acagacggcc cauguaaaau accaguacaa auggcaguag     1140

acaugcaaac acucacgccg gugggcagac ucauaacagc aaacccaguc auaaccgaaa     1200

gcacagaaaa caguaaaaug augcuugaac ucgacccacc cuucggugac ucguacaucg     1260

ucauaggagu aggcgacaaa aaaaucacgc accacuggca ccgaucaggg ucaaccauug     1320

gcaaagcauu cgaagcaaca guacgcgggg ccaaacgcau ggccguccua ggggacaccg     1380

ccugggacuu cggaucauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  7393
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 2 opt

<400>  7393
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

aaaaaguaau auaccuagua augauccucc ucaucgcacc ggccuauagu auccguugca      120

uaggagucag caacagagac uuuguagaag gcaugagcgg cggcaccugg gucgacgucg      180

uccuggaaca cggaggaugc gugaccguaa uggcacaaga caaaccgacc guggacaucg      240

aacuggucac aacaacaguc ucaaacaugg ccgaagucag aagcuacugc uacgaagcaa      300

gcauaagcga cauggcgagc gacagccgau gcccaaccca gggagaagca uaccucgaca      360

aacagagcga cacacaauac guaugcaaac gaacgcucgu agacaggggc uggggcaacg      420

gaugcggccu auucggaaaa gggagccuag ucacgugcgc gaaauucgcc ugcagcaaga      480

aaaugacagg aaaaucaauc cagccagaaa accuagagua ccgaaucaug cugagcguac      540

acgggagcca acacucaggc augaucguaa acgacaccgg acacgaaacc gacgaaaacc      600

gcgcaaaagu agaaauaacc ccaaacucac caagggccga agcaacgcua ggcggauucg      660

gcucacucgg acucgacugc gagccaagaa ccggacucga cuucagugac cuguacuacc      720

ucacaaugaa caacaaacac uggcuaguac acaaagaaug guuccacgac auaccacucc      780

ccuggcacgc gggcgcagac acgggcacac cacacuggaa caacaaagaa gcccucguag      840

aguucaaaga cgcacacgcc aaacgacaga ccgugguagu acucggaagc caggaagggg      900

ccgugcacac cgcccuggcc ggcgcccugg aagccgaaau ggacggagca aaaggccgcc      960

ucucaagcgg gcacuuaaaa ugucgccuaa aaauggacaa acucagguua aaaggcguaa     1020

gcuacagucu augcacggcc gcauucacau ucaccaaaau accagcggaa acgcuacacg     1080

gcacagucac ggucgaaguc caauacgcgg gcacagacgg gcccuguaaa guaccagcac     1140

aaauggccgu agacaugcaa acacuaacac cggucggccg acucauaacc gcgaacccag     1200

ucauaaccga aagcacagaa aacaguaaga ugaugcuaga acuagauccc ccauucggag     1260

acagcuacau agucauaggc guaggugaga aaaagauaac acaccacugg cacagaagcg     1320

gaucaaccau aggaaaggcc uucgaagcaa ccgugagagg ggcaaaaaga auggccguac     1380

ucggcgacac cgccugggac uucggcucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7394
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 2 opt

<400>  7394
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguaau cuaccuaguc augaucuugc uaauagcccc ggccuacagc auacgcugca      120

ucggaguauc aaacagggac uucgucgaag gaaugagcgg aggcacgugg guagacgugg      180

uacuagaaca cggcggaugc gucaccguaa uggcgcaaga caaaccaacc guggacaucg      240

aacucguaac gaccacggua ucaaacaugg ccgaaguaag aagcuacugc uacgaagcaa      300

gcaucagcga cauggcaagc gauagccggu gcccgaccca aggagaagca uaccuagaca      360

aacagagcga cacccaauac guaugcaaac gaacccuagu agacaggggc uggggcaacg      420

gcugcggacu auucggaaaa gggagccuag ucacaugcgc caaauucaca ugcagcaaga      480

aaaugaccgg gaaaucaauc cagcccgaaa accuagaaua ccgcauaaug cuaagcgugc      540

acgggaguca gcacucuggc augaucguga acgacacggg auacgaaaca gacgaaaaca      600

gagcaaaagu agaaguaaca ccaaacuccc caagagccga agccacgcuc gggggauucg      660

gaucgcucgg acuagacugc gaaccccgua ccgggcuaga cuucucagac cuauacuacc      720

uaacaaugaa caacaaacac uggcucguac acaaagaaug guuccacgac auaccacugc      780

ccuggcacgc gggcgccgac acuggaacac cacacuggaa caacaaagaa gcccuaguag      840

aguucaaaga cgcacaugca aagagacaga ccguggucgu acugggaagc caagaaggag      900

caguacacac agcacuagca ggggcacuag aagcggaaau ggacggggcc aaaggaaaac      960

uauucagugg acacuuaaag ugcagacuaa aaauggacaa acuaagacuc aagggcguaa     1020

gcuacagccu augcacagca gcguucacgu ucacgaaagu gccagcggaa acgcuacacg     1080

gaaccgucac ugucgaaguc caauacgcgg gcacagacgg gcccugcaaa auccccgucc     1140

aaauggccgu agacaugcaa acgcuaacac ccgugggaag acuaauaacc gcaaacccag     1200

ucauaacaga aagcacggaa aauagcaaaa ugaugcucga acuagaccca ccguucggag     1260

acagcuacau aguaauaggc guaggagaca agaaaaucac ccaccacugg caccgcucgg     1320

gcucaaccau aggaaaagcc uucgaagcga ccguacgcgg agccaaacga auggccguac     1380

uaggagacac ggcaugggac uucggcucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7395
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 2 opt

<400>  7395
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

agaaaguaau auaccuagua augauacucc ucauagcccc ggccuacucc auacgcugca      120

ucggagucag caacagagac uucguagagg gaaugagcgg cggaacaugg guagacgugg      180

uccuagaaca cggcggaugc guaacaguaa uggcacaaga caaaccaacc guggacauag      240

aauuggucac aaccacagua ucgaacaugg ccgaagucag aaguuacugc uacgaagcaa      300

gcauaagcga cauggcaagc gacagccgcu gccccaccca aggagaggca uaccucgaca      360

aacaaagcga cacacaauac guaugcaaac gaacacucgu agaccggggc ugggguaacg      420

gcugcggccu auucgggaaa gggagccuag ucacaugcgc gaaguucaca ugcagcaaga      480

aaaugaccgg caaaagcauc cagcccgaga accuagaaua cagaauaaug cucagcgugc      540

acgggagcca acacucgggc augaccguaa acgacaucgg cuacgaaaca gacgaaaacc      600

gcgccaaagu agaaguaacc ccgaacucgc caagagccga agccacccua gguggauucg      660

gaucccucgg acucgacugc gaaccaagaa ccggacucga cuucucggac cuauacuacc      720

ucacgaugaa caacaaacac uggcuaguac acaaagaaug guuccacgac auaccccuac      780

ccuggcacgc cggagcagac acaggcacgc cacacuggaa caacaaagaa gcgcuaguag      840

aguucaaaga cgcccacgcc aaacgacaaa cagugguagu guuaggaagc caagaagggg      900

caguccacac agcccuggca ggagcacucg aagcagaaau ggacggagcc aaaggcaaac      960

ucuuuagcgg acaccucaaa ugccgccuaa aaauggacaa acuccgccua aagggcguaa     1020

guuacagccu gugcacggca gcguucaccu ucacaaaagu gcccgcggaa acacuccacg     1080

gcaccgucac ggucgaaguc caauacgcag gaacggacgg acccugcaag auacccguac     1140

aaauggcagu agauaugcag acucucaccc ccgucggaag acucauaacc gccaacccgg     1200

uaauaacaga aagcacagaa aacagcaaaa ugaugcucga acucgaccca ccauucgggg     1260

acucauacau agugaucggc gugggcgaca agaaaaucac ccaccacugg caucgcucgg     1320

gcagcaccau aggcaaagcc uucgaagcaa caguccgcgg ggcaaaacga auggccguau     1380

uaggggacac cgcaugggac uucggcucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7396
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 2 opt

<400>  7396
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

aaaaggucau auaccuugua augauccuac ucauagcucc agcauacagc auacgcugca      120

uaggcguauc aaacagagac uucguagaag gcaugagcgg aggaacaugg gucgacguag      180

uacucgaaca cggaggcugc gucacaguaa uggcacagga caaaccgacc guggacauag      240

aacucgucac aaccacaguc uccaacaugg ccgaaguaag aagcuacugc uacgaagcaa      300

gcaucagcga cauggcguca gacagccgau gcccaacaca aggcgaagcc uaccuggaca      360

aacagaguga cacccaguac gugugcaaac ggacacuugu agacagaggc uggggcaacg      420

gcuguggccu guucggaaaa gggagccuag ugaccugcgc aaaauucaca ugcagcaaga      480

aaaugacagg caaaucaauc caaccagaaa accucgaaua cagaauaaug uuauccgucc      540

acggaagcca acacuccggc augauaguaa acgacgaaaa ccgcgccaaa guagaaguaa      600

caccaaacuc acccagagcc gaagccacgc ucggcggcuu cggaucgcua ggccuagacu      660

gcgaaccccg aacagggcuc gacuucagcg accucuacua ccucacgaug aacaacaaac      720

acuggcuagu acacaaagaa ugguuccacg acaucccccu acccuggcac gcaggcgcag      780

auacaggcac accgcacugg aacaacaagg aagcgcucgu ggaguucaaa gacgcacacg      840

ccaagagaca aaccguaguc guacuaggaa gucaggaagg cgccguacac accgcccucg      900

ccggagcacu cgaagcagaa auggacggag caaaaggaag acucuucagc ggacaccuca      960

aaugccgccu caaaauggac aagcuaagac ucaaaggcgu aagcuacagc cuaugcacag     1020

ccgccuucac auucacaaaa guacccgccg aaacacucca cggcacaguc accgucgaag     1080

uccaauacgc cggcacagac ggacccugca aaguacccgc acaaauggcc gucgacaugc     1140

aaacgcucac acccgucggc aggcucauaa ccgcaaaccc ggucaucacc gaauccacgg     1200

aaaacaguaa aaugaugcuc gaacuagauc ccccauucgg cgacucguac auaguaauag     1260

gcgucggcga caagaaaauu acacaccacu ggcaccgauc cggaagcacc auaggcaaag     1320

ccuucgaagc aacaguccgc ggcgccaaac gcauggccgu acuaggggac acggcauggg     1380

acuuuggcuc gugaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  7397
<211>  1729
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 2 opt

<400>  7397
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaagucau auaccuagua augauacugc uaauagcacc agcauauagc auacgcugca      120

ucggagucuc caacagagac uucgucgagg gcaugagcgg gggaacgugg guggacguag      180

uacucgaaca cggcggaugc gucaccguca uggcacaaga caaaccaaca guagacauag      240

aacuggucac aaccaccguc ucaaauaugg ccgaagucag auccuacugc uacgaagcaa      300

gcaucuccga cauggcaagc gacagccgau gcccaaccca aggcgaagcc uaccucgaca      360

aacaaucaga cacgcaauac guuugcaaac gaacccucgu cgaccgaggc uggggcaacg      420

gcugcggccu cuuugggaaa ggaagccuag uaacaugcgc gaaauucaca ugcuccaaga      480

aaaugaccgg caaaucaauc caaccagaga accuagaaua ccgaaucaug cugagcguac      540

acggcagcca acacucagga augauagggu acgaaaccga cgaaaaucga gccaaagucg      600

aagucacccc aaacagccca cgagccgaag ccacccucgg aggcuucgga ucacucggac      660

ucgacugcga accccggacc gggcucgacu ucucagaccu guacuaccuc acaaugaaca      720

auaaacacug gcucguacau aaagaauggu uucacgacau accccucccc uggcacgccg      780

gcgcagacac cggcacacca cacuggaaca acaaagaagc acucguggag uucaaagacg      840

cccacgccaa acgccagacc gucgucguac uaggcucaca agaaggagca guccacacag      900

cccuagcagg ggccuuagaa gcggaaaugg acggcgcaaa agggaaacuc uucagcggac      960

accugaaaug ccgccuaaaa auggacaaac uacgacucaa aggcguaagc uacagucuau     1020

gcacggccgc auucaccuuc accaaagucc cagcagaaac acuccacggc acagucacag     1080

uagagguuca auacgcaggc acagacggcc ccugcaaaau acccguccaa auggccguag     1140

acaugcaaac ccuaaccccc gucggcagac uaaucacagc caacccgguc aucacagaau     1200

ccacagaaaa cagcaaaaug augcucgaac ucgacccacc auucggagac ucuuacauag     1260

ucauaggagu aggcgacaag aaaaucacgc accacuggca ccgauccggc uccacaauag     1320

gcaaagccuu cgaagcaacc gugcgcggcg caaaaagaau ggcaguccuc ggagacaccg     1380

ccugggacuu cggcucauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     1440

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     1500

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     1560

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1680

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 1729


<210>  7398
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 2 opt

<400>  7398
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

agaaagucau auaccugguc augauccugc ucauagcccc agccuacagu cuacgaugca      120

ucggaguaag caacagagac uucgucgaag gaaugagugg aggcacaugg guggacgucg      180

uccuggaaca cggcgggugc gucacaguaa uggcacaaga caaacccacc guagacaucg      240

agcuagucac aaccacaguc uccaacaugg ccgaagucag aagcuacugc uacgaagcaa      300

gcauaucaga cauggcaagc gacagcagau gcccgaccca gggggaggcc uaccucgaca      360

aacaaagcga cacccaauac gucugcaaac gcacccuagu ggacaggggc uggggcaaug      420

gcugcggccu guucggaaaa ggcagccugg ucacaugcgc aaaauucgcc ugcucaaaga      480

aaaugacagg aaaaagcauc caacccgaaa accuggaaua ucgcauaaug cuaucagucc      540

acggcagcca gcacucaggc augauaguga acgacaccgg acacgaaaca gacgaaaacc      600

gcgcaaaagu agaaauaacc ccaaacucac cccgagccga agcaacgcuu ggcggcuucg      660

gaucacuugg acucgacugc gaaccaagaa ccggacuaga cuucagugac cucuacuacc      720

ucaccaugaa caacaaacac uggcucgugc acaaagaaug guuccacgac auaccauuac      780

ccuggcacgc gggcgcagac acuggaacac cccacuggaa caacaaagaa gcgcucgugg      840

aguucaaaga ugcccacgcc aaacgccaga ccguggucgu acucgggagc caggaaggag      900

caguacacac ggcccuagca ggggcccuag aagcagaaau ggacggagca aaaggcagac      960

ucucaagcgg acacuuaaaa ugccgccuga aaauggacaa acuacggcua aaaggaguaa     1020

guuacagccu cugcacagcc gcauucacgu ucacgaagau accggccgaa acacuccacg     1080

gaacagucac gguagaaguc caauacgcgg gcacggacgg gccaugcaaa guacccgcgc     1140

aaauggcagu agacaugcaa acgcuaacac ccgugggccg acucauaaca gcaaaccccg     1200

ucauaaccga aagcaccgaa aacagcaaaa ugaugcucga acucgauccc ccguucggag     1260

acucauacau aguaauaggc guaggagaaa agaaaaucac gcaccacugg caccgcucag     1320

gaucaaccau aggcaaagcc uucgaagcca caguaagagg ggccaaacgc auggcagugc     1380

uaggcgacac agccugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7399
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 2 opt

<400>  7399
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagca       60

aaaaagucau auaccucguc augauccucc uuauagcccc cgccuacuca auccgcugca      120

ucgguguaag caaccgcgac uucguugaag gaauguccgg cggaaccugg gucgacgucg      180

uccucgaaca cggcggcugc gugacuguca uggcacaaga caaacccacc guagacaucg      240

aacucgucac aaccaccguc ucgaacaugg ccgagguacg gagcuacugc uacgaagcau      300

ccauaucaga cauggccucc gacuccagau gcccaacuca aggagaagcc uaccucgaca      360

aacaauccga cacccaauac guaugcaaac gaacccuagu ggaccgagga uggggcaacg      420

gcugcggacu cuucggcaaa ggcucccucg ugacaugcgc caaauucgcc ugcucaaaaa      480

aaaugaccgg aaaauccauc caacccgaaa accucgaaua caggaucaug cuaagcgucc      540

acggaucaca acacuccgga augaucguca acgacacagg acacgaaaca gacgaaaacc      600

gagcaaaagu agaaauaaca ccaaacuccc ccagagccga agccacccuc ggcggcuucg      660

gcucccuagg ccuagacugc gaaccaagaa cgggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaaacac uggcucguac acaaagaaug guuccacgau aucccccucc      780

ccuggcacgc cggcgccgac accgggaccc cccacuggaa caacaaagaa gccuuagucg      840

aauucaaaga cgcacacgca aaaagacaaa ccgucgucgu ccucggaagc caagaaggcg      900

caguccacac agcccucgcg ggagcccuag aagcagaaau ggacggagcg aaaggccgcc      960

ucuccagcgg ccaccucaaa ugccgccuca aaauggacaa acuaagacug aaaggcgucu     1020

ccuacucccu cuguaccgca gcauucaccu ucacaaaaau ccccgcagaa acccuccacg     1080

gcaccgucac cguagaaguc caauacgcag gcacggacgg ccccugcaaa guaccagccc     1140

aaauggccgu agacaugcaa acccuaaccc ccgucggccg acucauaacc gcaaauccag     1200

ucaucacaga aucgaccgaa aacagcaaaa ugaugcucga acuugacccc ccauucgggg     1260

acuccuacau aguaaucggc gucggcgaaa aaaagauaac ccaccacugg caccgcucug     1320

gaucuaccau cggcaaagcc uucgaagcca ccguacgcgg agcaaaacgc auggccgucc     1380

ucggcgacac cgcaugggac uucggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7400
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 2 opt

<400>  7400
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau cuaccugguc augauccuac ucauagcccc cgcauacagc auacgcugca      120

uaggagucuc aaaccgcgac uucgucgaag gaaugagcgg gggaacgugg guagacguag      180

uccuagaaca cggcggaugc guaaccguaa uggcgcaaga caaaccaacc guagacauag      240

agcuagucac aacaacaguc ucgaacaugg cagaagucag gagcuacugc uacgaagcaa      300

guauaagcga cauggccagc gauucccgau gcccgacaca aggagaagca uaccuagaca      360

aacaaagcga cacacaauac guaugcaaac gcacccuagu agaccguggc uggggcaacg      420

guugcggacu auucgggaaa ggaagucugg ucacaugcgc aaaauucgca ugcagcaaga      480

aaaugaccgg aaaaucgaua cagcccgaaa accuagaaua ccgaauaaug cuaagcguac      540

acgggagcca acacucggga augauaguaa acgacaccgg acacgagacc gacgaaaaca      600

gagcaaaagu agaaauaaca ccaaacucgc cucgagccga agcaacacuc ggaggcuucg      660

gaucacucgg acucgacugc gagcccagaa ccggccucga cuucucggac cuauacuacc      720

ucacaaugaa caacaaacac uggcucguac acaaagaaug guuccacgac auaccccugc      780

ccuggcacgc aggcgccgac acgggaacgc cccacuggaa caacaaagaa gcccuaguag      840

aguucaaaga cgcacacgcc aaacgccaaa ccguggucgu gcucgggagc caagaaggag      900

cagugcacac agcacuggcc ggagcccugg aagccgaaau ggacggagca aaaggaaggc      960

ucucaagcgg acaccuaaaa ugccgccuga aaauggacaa guuaaggcua aaaggaguau     1020

cauacagccu cugcacggcc gcguucaccu ucacaaaaau acccgcagaa acgcuacacg     1080

gcaccgucac agucgaagua caguacgcgg gaaccgacgg cccaugcaaa guacccgcac     1140

aaauggccgu ggacaugcaa acacuaacgc cagugggcag gcuaauaacc gcaaacccag     1200

ucauaacgga aagcacggaa aauagcaaaa ugaugcucga acucgaccca ccguucggag     1260

acucguacau aguaauaggc guaggcgaaa agaaaauaac acaccacugg uaccgcucgg     1320

gcucaaccau aggcaaagca uucgaagcaa ccgugcgagg cgccaaacgc auggcuguac     1380

ucggagacac agcaugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7401
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 2 opt

<400>  7401
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auaccuaguc augauccuac uaaucgcccc agcauacuca auacgcugca      120

ucggcgucag caaucgagac uucgucgaag gcaugagcgg cggcacaugg gucgacguag      180

uccucgaaca cggcggcugc gucaccguca uggcccaaga caaaccaacc guagacauag      240

aacucgucac aacaacaguc ucaaacaugg ccgaagucag aagcuacugc uacgaagccu      300

ccauaagcga cauggcaucc gacucacgcu gcccaacaca aggcgaagca uaccucgaca      360

aacaguccga cacgcaauac guaugcaaac gcacccuagu agacaggggc ugggggaacg      420

gaugcggcuu auucggcaaa ggcagccucg ucacaugcgc gaaauucgcc ugcuccaaga      480

aaaugaccgg caaauccaua cagccagaga accuagaaua ccgaauaaug cucuccgucc      540

acggaucaca acacagcgga augaucguaa acgacaccgg ccacgagacc gacgaaaacc      600

gcgccaaagu cgaaaucacc cccaacagcc cacgagccga ggccacacuc ggaggcuucg      660

gcucgcuagg acucgacugu gaaccuagaa ccggccucga cuucucagac cucuacuacc      720

uaacgaugaa caacaaacac uggcuagugc acaaagaaug guuccacgac auaccacugc      780

ccuggcacgc aggcgcagac accggcacgc cacacuggaa caacaaggaa gcccuaguag      840

aguucaaaga cgcccacgcc aagcgacaaa ccgucguagu gcucggcucc caagaaggag      900

ccguccacac agcucuagcc ggagcacuag aagcagaaau ggacggagcc aaaggaaaac      960

ucucaagcgg ccaccuaaaa ugccgacuca aaauggacaa auuacgacua aaaggcguau     1020

ccuacucccu augcacagcc gcauucacau ucacaaaaau ucccgccgaa acgcuacacg     1080

gcacagucac aguggaagua caauacgccg gcacagacgg accaugcaaa guaccagccc     1140

aaauggccgu cgacaugcaa acgcugacac ccgucggcag acucauaaca gccaaccccg     1200

ucauaaccga aaguaccgaa aacucaaaaa ugaugcucga acucgaccca ccauucggag     1260

acagcuacau agucaucggc gucggagaaa agaaaauaac ccaccacugg caccgaagcg     1320

gcuccacaau cggaaaagcc uucgaagcca caguacgggg agccaaacgu auggccguac     1380

ucggcgauac cgcaugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7402
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 2 opt

<400>  7402
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaagucau auaucuaguc augauccugc uaauagcccc agccuacagc auccgcugca      120

ucggaguauc gaaccgcgac uucgucgaag gcaugagcgg aggcaccugg guagacguag      180

uccuagaaca cggcggaugc guuaccguaa uggcacaaga caagccaacc guagacaucg      240

agcuagucac caccaccguu uccaacaugg cagaaguacg aagcuacugc uacgaagcaa      300

guauaagcga cauggcgucc gacagccggu gccccacaca aggcgaagcc uaccucgaca      360

aacaaagcga cacacaguac guuugcaagc ggacgcuggu ggaccgcggc uggggcaacg      420

gaugcggauu guucggaaaa ggaagccuag ucacaugcgc gaaauucgca ugcagcaaga      480

aaaugacagg caaaucaaua cagccagaaa accuagaaua cagaauaaug cuaagcguac      540

acggaaguca acacagcggc augaucguca acgacacagg ccacgagacc gacgaaaaca      600

gagcaaaagu agaaauaacc ccaaacucgc caagagccga agccacacuc ggaggauucg      660

gcucgcucgg acucgacugc gagcccagaa ccggacucga cuucuccgac cuauacuacc      720

ucacaaugaa caauaaacac ugguuaguac acaaagaaug guuccacgac aucccacugc      780

ccuggcacgc aggagccgac acaggcacac cccacuggaa caacaaggaa gcucuaguag      840

aguucaaaga cgcccacgcg aaacgucaaa ccguaguagu acuaggcagc caagaaggcg      900

cagugcacac agcccucgcc ggcgcacuag aagcggaaau ggacggcgca aaaggcagau      960

uaagcagcgg ccaccuaaaa ugccgccuaa agauggacaa acuaagacuc aaaggaguaa     1020

gcuacagccu augcacagca gcauucaccu ucaccaaaau acccgccgaa acacuacacg     1080

ggacaguaac agucgaagua caguaugcag gcacagacgg ccccugcaaa guacccgcac     1140

aaauggccgu ggacacccaa acacuaaccc ccguaggcag gcuaauaacc gccaacccag     1200

uaaucaccga aagcacagaa aacagcaaaa ugaugcucga acuagauccc ccauucggag     1260

acuccuacau cgucauagga gucggcgaaa agaaaaucac ucaucacugg caccgaucag     1320

gcuccaccau cggcaaagca uucgaagcaa ccguacgcgg cgccaaacgu auggccgugc     1380

uaggcgacac agccugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7403
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 2 opt

<400>  7403
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaagucau auacuuaguc augauacuac ucauugcccc cgcauacucc auacgaugca      120

ucggcgucuc caacagagac uucguagaag gcaugagcgg cggcacaugg gucgacguag      180

uccuggaaca cggcggcugc guaaccguga uggcccaaga caaaccaacu guagacauag      240

aacucgucac aaccaccgua agcaacaugg ccgaaauccg aagcuacugc uacgaagcca      300

gcauaagcga cauggcgucc gacagccgcu gcccaaccca aggcgaagcc uaccuagaca      360

aacagagcga cacacaauac gucugcaaac gcacacuagu agacagaggc uggggaaacg      420

gcugcggacu auucggaaaa ggaagccuag uaacgugcgc gaaauucgcc ugcagcaaaa      480

aaaugacagg aaaaucaauc cagcccgaga accuagaaua cagaauuaug cuaagcguac      540

acggguccca acacucagga augauaguaa acgacacugg ccacgaaacc gacgaaaaca      600

gagccaaagu agaaauaaca cccaacucac cccgagccga agccacacua ggaggcuucg      660

gcucacucgg acucgacugc gaaccaagaa ccggacuaga cuucucggac cucuacuacc      720

ucacgaugaa uaacaaacac uggcucgucc acaaagaaug guuccacgac auaccccucc      780

cuuggcacgc uggcgcagac accggaacac cacacuggaa caacaaggaa gcacucgucg      840

aauucaaaga cgcccacgcc aaacgccaaa ccguagucgu acucggaucc caagaagggg      900

caguacacac agcccuggcc ggagcacucg aagcagaaau ggacggcgcc aaaggacgac      960

uaucuagugg ccaccugaaa ugucgccuaa aaauggacaa acuccgacuc aaaggcguaa     1020

gcuacagccu cugcaccgca gccuucaccu ucaccaaaau accagcagaa acacuacacg     1080

gcacgguaac cgucgaaguc caguacgcag gaacugacgg gcccugcaaa gucccagccc     1140

aaauggcagu cgacaugcaa acccuuaccc ccguaggacg ccucauaaca gccaacccag     1200

ucaucaccga aagcacagaa aacagcaaaa ugaugcucga acucgaccca cccuucggag     1260

acucauacau agucauuggc gugggcgaaa aaaaaauaac ucaccacugg caccgaucgg     1320

gcucaacuau aggcaaagcc uucgaagcga ccguccgcgg cgccaaacgc auggccguac     1380

ucggcgacac agccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7404
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 2 opt

<400>  7404
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau auaccucguc augauccugc ucauagcccc cgcuuacucc auacgcugca      120

ucggcgucag caacagggac uucguagaag gcaugucagg cggcacaugg guagacguag      180

uccuagaaca cgggggaugc gucaccguaa uggcccaaga caaaccaaca guagacaucg      240

aacucgucac gacgaccguc uccaacaugg ccgaaguacg cagcuacugc uacgaagcaa      300

gcauaucaga cauggcaucc gacucccgcu gccccaccca aggcgaagcc uaccucgaca      360

aacaauccga cacccaauac guaugcaaac gaacccuagu agaccgaggc uggggaaacg      420

gcugcggacu auucggaaaa ggcucacucg uaacaugcgc aaaauucgcc ugcagcaaga      480

aaaugaccgg aaaauccauc caacccgaaa accucgaaua ccgcauaaug cucuccgucc      540

acggaagcca acacucgggc augaucguaa acgacaccgg ccacgaaacc gacgaaaacc      600

gcgcaaaagu agaaauaaca cccaacagcc ccagagcaga agcaacccuc ggcggcuuug      660

gaucccucgg acuagacugc gaaccccgaa ccggccucga cuucagcgau cuauacuacc      720

ucacaaugaa caacaaacac uggcuagucc acaaagaaug guuccacgac auaccucugc      780

ccuggcacgc cggugcagac acaggcaccc cgcacuggaa caacaaagaa gcccuagucg      840

aguucaaaga cgcccacgcc aaacgccaaa cagucgucgu ccuaggcagc caagaaggcg      900

caguccacac cgcccuagca ggagcccuag aagcagaaau ggacggagca aaaggaagau      960

uaucauccgg ccaccugaaa ugucgccuaa aaauggacaa acuccgacuc aaagggguca     1020

gcuacagccu cugcacggcc gcauucacau ucacaaaaau cccagcagaa acacuccacg     1080

gaaccgucac aguagaaguc caauacgcag gcaccgacgg ccccugcaaa guaccagcac     1140

aaauggccgu agacaugcaa acccuaaccc ccgucggacg ccuuaucacc gcaaacccag     1200

uaaucacgga aucaaccgaa aacagcaaaa ugaugcuaga acucgacccg cccuucggcg     1260

acagcuacau aguuauaggc guaggagaaa agaaaauaac ccaccacugg caucgcagcg     1320

guucaaccau cggaaaagcc uucgaggcca ccguacgcgg cgcacgccgc auggccguac     1380

ucggcgacac ggccugggac uucggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7405
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 2 opt

<400>  7405
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau auaccuaguc augauacuac ucaucgcacc cgccuacuca auccgcugca      120

uaggcgucuc aaaccgagac uucgucgaag gcaugagcgg aggaacgugg gucgacguag      180

uacucgaaca cggcggaugc gugaccguua uggcacaaga caaaccaacc gucgacauag      240

aacucgucac aacaacgguc ucaaacaugg ccgaaguccg cucauacugc uacgaagcau      300

ccauaagcga caugguaucu gacucacgcu gcccaaccca gggagaagcg uaccuagaca      360

aacaaagcga cacccaauac gucugcaaaa gaacccuagu ggaccgcgga uggggaaacg      420

gcugcggccu auucggcaaa ggaagccuag uaacaugcgc caaauucgcc ugcagcaaga      480

aaaugaccgg caaaucaaua cagcccgaaa accuagaaua cagaauaaug cuaagcgucc      540

acggcucaca acacuccgga augaucguaa acgacaccgg acaugaaacc gacgaaaacc      600

gagcaaaagu cgaaaucacc ccaaacucac ccagagccga agccacccua ggaggauucg      660

gcagccuagg ccuagacugc gaaccacgaa ccggacucga cuucuccgac cucuacuacc      720

uaacaaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac aucccccucc      780

ccuggcacgc aggagcagac accggcacac cccacuggaa caacaaagaa gcgcucgucg      840

aguucaaaga cgcacacgcc aaacgccaaa ccgucgucgu acuaggaagu caagaaggcg      900

ccguacacac cgcacucgca ggcgcacuag aagcagaaau ggacggagcc aaaggccgac      960

ucagcagcgg acaccucaaa ugccgacuaa aaauggacaa gcuacgccuc aaaggagucu     1020

ccuacagccu augcacagca gcguuuacau ucacgaaaau cccagcagaa acccuccacg     1080

gcacagucac ggucgaagua caauacgcag gcacagacgg acccugcaaa guccccgcgc     1140

aaauggccgu agacaugcaa acccugaccc caguaggccg ccuuaucacc gccaacccag     1200

ucaucaccga auccacagaa aacuccaaaa ugaugcucga acuagauccc ccguucggag     1260

acuccuacau aguaauaggc guaggagaaa agaaaaucac ccaccacugg cacagaucgg     1320

gcuccacaau cggcaaagca uucgaagcaa ccguccgagg cgccaaacgc auggccguac     1380

ucggagacac ggcaugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7406
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 2 opt

<400>  7406
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau auaccucguc augauccuuc ucaucgcacc agcauauucc auacgcugca      120

uuggaguauc aaacagagac uucgucgaag gaaugagcgg aggcacgugg guggacgugg      180

uccuagaaca cgggggcugc guaaccguaa uggcucaaga caaaccgaca guagacaucg      240

agcuagucac caccaccguc ucaaacaugg cagaagucag aaguuacugc uacgaagcca      300

gcauaagcga cauggcaagc gacagcagau gccccaccca aggcgaggca uaccucgaca      360

aacaaagcga cacccaauac guaugcaaac gcacacucgu agaccgcggc uggggcaacg      420

gcugcggacu auucggcaaa ggaagccucg ucacaugcgc caaauucgca ugcucaaaga      480

aaaugaccgg caaaagcaua cagcccgaga accuggaaua ccgaauaaug cucagcguac      540

acggaucaca acacucaggc augaucguga acgacacagg gcaugaaacc gacgaaaacc      600

gcgccaaagu agaaaucacc ccgaacuccc caagagccga agccacacuc ggcggcuucg      660

gcucacuagg acucgacugc gagccaagaa ccggccuaga cuucucagac cuuuacuacc      720

ucacaaugaa uaacaaacac uggcucgucc acaaagaaug guuccacgac auaccccucc      780

ccuggcacgc aggcgcagau acaggaacgc cccacuggaa caacaaggaa gcacuuguag      840

aguucaaaga cgcacacgcc aaacgacaaa ccguagucgu acucggaagc caagaaggag      900

ccgugcacac cgcccuagca ggagcccucg aagccgaaau ggacggagcc aaaggcagac      960

uaucaagcgg acaccuaaaa ugccgccuaa aaauggacaa acuacgacuc aagggcguau     1020

ccuacagccu augcaccgcu gcauuuaccu uuacaaaaau acccgccgaa acacuacacg     1080

gcacaguaac agucgaaguc caguacgcag gcacagacgg gcccugcaaa guaccagcac     1140

aaauggcagu agacaugcaa acgcuaacgc ccguaggacg ccucaucaca gccaacccag     1200

ucaucaccga aagcauagaa aacagcaaaa ugaugcuaga acuagauccc ccauucggag     1260

acucauacau agucaucggc guaggagaaa agaaaaucac gcaccacugg caccgcucag     1320

gaucaaccau cggcaaagcc uuugaagcaa cagugcgagg cgccaaacgc auggccguac     1380

ucggagacac agccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7407
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 2 opt

<400>  7407
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguaau auaccuaguc augauacugc ucauagcacc agccuacagu auacgaugca      120

ucggcgucag caacagggac uucgucgaag gcaugagcgg aggcacaugg guugacgugg      180

uacuagaaca cggcggaugc guaaccguga uggcacaaga uaaaccaacg guagacaucg      240

aacugguaac aacaacagua agcaacaugg ccgaaguaag gagcuacugc uacgaagcaa      300

gcauaagcga cauggcaagc gacuccagau gcccaacaca aggcgaagcc uaccucgaca      360

aacaaagcga cacccaauac gucugcaaac gcacacucgu agauagaggc uggggaaaug      420

gcugcggccu auucggaaaa ggaagccucg ucacgugcgc aaaauucgcc ugcagcaaga      480

aaaugaccgg caaaucgauc caaccagaaa acuuagaaua ucgaaucaug cugagcguac      540

acgggagcca acacuccgga augauaguga acgacacagg acacgaaacc gacgagaaca      600

gggcaaaagu agaaaucaca ccaaacucac cccgggccga agccacacuc ggaggauucg      660

gaagccucgg acuagacugc gaaccaagaa caggacucga cuucagugac cuauacuacc      720

ucacaaugaa caacaaacac uggcucguac acaaagaaug guuccacgac aucccacugc      780

cauggcacgc gggcgcagac acaggcacac cccacuggaa caacaaagaa gcccuaguag      840

aguucaaaga cgcacacgcc aaacgccaaa ccguagucgu acuaggaagc caagaagggg      900

ccguacacac agcccuggcc ggggcacucg aagcagaaau ggacggagca aaaggcaggc      960

ucucgucagg acaucuaaaa ugccgccuaa aaauggacaa acucaggcuc aaaggcguga     1020

gcuacagccu guguacagca gcauucacau ucacaaaaau acccgcagaa acacuccacg     1080

gcacagucac gguagaagua caauacgcag gcacagacgg cccaugcaaa guacccgcgc     1140

agauggccgu cgacaugcaa acacuaacac ccguaggacg acuaauaacc gcaaacccgg     1200

ucauaaccga aagcacggaa aauagcaaaa ugaugcucga acuagaccca ccauucggcg     1260

auucauacau cgucaucggc auaggcgaaa agaaaaucac acaccacugg caccgcucag     1320

gcucaacaau aggcaaagcc uuugaagcaa ccguacgcgg ggcaaaacgc auggcagugc     1380

uaggggacac agcaugggac uucggcagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7408
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 2 opt

<400>  7408
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

agaaagucau cuaccuaguc augauccucc uaaucgcacu cgccuacuca auacgcugca      120

uaggagucuc caaccgagac uucguagaag gaauguccgg cggaaccugg guagacguag      180

uccucgaaca cgggggcugc guaacaguca uggcccaaga caaacccacc gucgacauag      240

aacucguaac caccaccguc ucaaacaugg ccgaaguccg aucguacugc uacgaagcca      300

gcaucuccga cauggccucc gacucccgau gcccaaccca gggagaagcc uaccuagaca      360

aacaaagcga cacacaauac guaugcaaac gaacacucgu cgaccgcggc uggggaaacg      420

guugcggacu auucggcaaa ggaagccuag ucacaugcgc aaaauucgca ugcucaaaga      480

aaaugaccgg caaauccauc caaccagaaa accucgaaua ccgaauaaug cucagcgucc      540

acggcucaca acacagcgga augauaguaa acgacaccgg acacgaaacc gacgaaaacc      600

gcgcaaaagu cgaaaucacc cccaacuccc cacgcgccga agccacccua ggaggauucg      660

gaucccucgg acuagacugc gaacccagaa ccggccucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaaacac ugguuagucc acaaagaaug guuccacgac aucccccucc      780

ccuggcacgc cggcgccgac acaggaaccc cgcacuggaa caacaaagaa gcccucgucg      840

aguucaaaga cgcgcacgcc aaacgacaaa ccgucgucgu acuagggucc caagaaggag      900

ccguacacac agcacuagcc ggcgcccuug aagcagaaau ggacggcgcu aaaggccgac      960

ucagcuccgg acaccucaaa ugcagacuua aaauggacaa acuccgccuc aaaggcguca     1020

gcuacagccu cugcaccgcc gcauucacau ucacaaaaau acccgccgaa acccuccacg     1080

gcaccgucac aguagaaguc caauacgccg gaaccgacgg ccccugcaaa guaccagccc     1140

aaauggcagu cgacaugcaa acacuaaccc cagucggacg acuuaucacg gcaaaccccg     1200

ucaucacaga aucaaccgaa aacuccaaaa ugaugcucga acucgaccca ccuuucggcg     1260

acuccuacau cguaaucgga guaggcgaaa agaaaaucac acaccacugg caccgauccg     1320

gauccacgau cggcaaagcc uucgaagcaa caguccgcgg agcaaaaaga auggccguac     1380

ucggagauac cgccugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7409
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 2 opt

<400>  7409
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auaccuaguc augauccucc ucauagcccc agcauacucc auacgcugca      120

ucggaguaag caaccgagac uucgucgaag gaaugucagg cggaaccugg gucgacgucg      180

uacuagaaca cggcggaugc guaacaguaa uggcccaaga caaaccaaca guagacaucg      240

aacucguaac aaccacaguc agcaacaugg cagaaguaag auccuacugc uacgaagccu      300

caauaagcga cauggcaucc gacuccaggu gcccaacaca aggcgaagcc uaccucgaca      360

aacaaagcga cacacaauac gucugcaaaa gaacacuugu agacagagga uggggcaacg      420

gaugcggccu cuucggaaaa ggaagccucg uaaccugcgc caaauucgcc ugcagcaaaa      480

agaugacagg aaaaucgauc caacccgaaa accuagaaua ccgcaucaug cucuccgucc      540

acggcagcca gcacucaggc augauaguca acgacacagg acacgaaaca gacgaaaacc      600

gagccaaggu agaaauaacc ccaaacagcc cccgagccga agcaacccuc ggaggauucg      660

gaucacuagg acuagacugc gaaccccgaa ccggccucga cuucuccgac cuauacuacc      720

uaaccaugaa caacaaacac uggcucgucc auaaggaaug guuccacgac auaccccugc      780

ccuggcacgc gggcgcagac acgggcacac cccacuggaa caacaaagaa gcccucgucg      840

aguucaaaga cgcccacgca aaacgccaga ccguagucgu acuaggcacc caagaaggag      900

ccguccacac cgcccuggcc ggagcccucg aagccgaaau ggacggagca aaagggcgac      960

ucagcagcgg ccaccuaaaa ugccgccuaa aaauggacaa acuaagacua aaaggcguau     1020

cauacagccu augcacagca gcauucaccu ucaccaaaau cccugcagaa acacuccacg     1080

gcaccgucac ggucgaaguc caguacgccg gcacagacgg acccugcaaa gucccagccc     1140

aaauggccgu cgacaugcaa acccuaacac ccgucggaag acuaauaacc gccaaccccg     1200

uaaucaccga aagcacagaa aauucaaaaa ugaugcucga acuagauccc ccguucggag     1260

acuccuacau aguuaucggc guaggcgaga aaaagaucac acaccacugg cacagauccg     1320

gcagcacaau aggaaaggcc uucgaagcaa caguccgcgg agcaaaacga auggcagucc     1380

ucggagacac agccugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7410
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 2 opt

<400>  7410
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaagucau cuaccuaguc augauccucc uaauagcccc ggccuacagu auacgaugca      120

ucggcgucag caacagggac uucgucgaag gcaugagcgg gggcacgugg guagacguag      180

uuuuagaaca cggcggaugc guaaccguga uggcacaaga caagccaacc guagacauag      240

agcuagucac aaccacaguu ucaaacaugg cagaagucag gagcuacugc uacgaagcga      300

gcauaagcga cauggcgagc gacagcagau gcccaacuca aggcgaagcc uaccuugaca      360

aacaaagcga cacccaguac guaugcaaaa gaacccuagu cgaccgagga ugggggaacg      420

guugcggacu auucggaaaa gggagccuag ucacaugcgc gaaauucgcc ugcagcaaaa      480

agaugacagg aaaaagcauc caacccgaga accuagaaua ucgaauaaug cucagcguac      540

acgggagcca acacucagga augauaguca acgacacagg gcacgaaacc gacgaaaacc      600

gagcgaaagu ggagauaacu ccaaacucac ccagagcuga ggcaacacua ggaggcuucg      660

ggucacuugg acuagacugc gaacccagaa caggccucga cuucucggac cuauacuacc      720

uaacaaugaa caacaaacac uggcucgugc acaaagaaug guuccacgac auaccacuac      780

cuuggcacgc aggagcagac acagguacac cacacuggaa caacaaggaa gcacucguag      840

aguucaaaga cgcacaugcc aaacgacaga ccguagucgu acucgggagc caagaagggg      900

ccguacacac ggcccuggca ggagcacuag aagcagaaau ggacggagcc aagggccgac      960

ucagcagcgg ccaccucaag ugcagauuga aaauggacaa gcuacgacuc aaaggcguaa     1020

gcuacagucu augcaccgcc gcauucacau ucacaaaaau cccagcagaa acgcuacacg     1080

gcacagucac cgucgaaguu caguacgccg gaacagacgg accaugcaaa gugcucgccc     1140

agauggcagu cgacaugcaa acgcuaacgc ccguagguag gcucauaacc gcgaacccgg     1200

uaaucacaga aagcacagaa aacagcaaaa ugaugcucga gcucgaccca cccuucggag     1260

acagcuacau cgucauaggg gugggagaaa agaaaaucac acaccacugg caccgcagcg     1320

guucaaccau cggcaaagcc uucgaagcga ccguacgagg agcuaaaaga auggccguac     1380

ucggagacac agccugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7411
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 2 opt

<400>  7411
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

agaaagucau cuaccuaguc augauccugc ucauagcacc agcauacagc auaagaugua      120

ucggagucuc caacagagac uucgucgaag gaaugagcgg aggaaccugg guagacguag      180

ucuuagaaca cgguggcugc gucaccguga uggcacaaga caaaccgaca guagacauag      240

aacucgucac aacaacagua agcaacaugg cagaaguacg aagcuacugc uacgaagcgu      300

ccauaagcga cauggccagc gacagcagau gcccaacaca aggcgaagca uaccuagaca      360

aacaauccga cacacaguac gucugcaaac gcacccuagu agacagaggg uggggcaacg      420

gaugcggacu auucgggaaa gggagccuag ucacaugcgc caaauucgcc ugcucaaaga      480

aaaugacagg aaaaagcaua caaccagaaa accuagaaua ccgaauaaug cuaagcguac      540

acggaagcca acacagcggc augaucguaa acgacaccgg acacgaaaca gacgaaaacc      600

gagcaaaagu cgaaauaaca cccaacagcc cccgagccga ggccacacua ggcgguuucg      660

gaagccucgg acucgacugc gaaccacgca cagggcuaga cuucuccgac cucuauuacc      720

uaacaaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auaccccuac      780

ccuggcacgc aggcgcggac acaggaaccc cgcacuggaa caacaaagaa gcccuagucg      840

aguucaaaga agcccacgcc aaacgacaga caguagucgu ccuaggaagc caagaaggag      900

ccguccacac agcccuagca ggagcacucg aagcagaaau ggacggggcc aaaggacgcc      960

uaagcuccgg acaucuaaaa ugccgccuaa aaauggacaa acucagacua aaaggaguca     1020

gcuacagccu augcacagcc gccuucaccu ucaccaaaau accggcagaa acacuccacg     1080

gcacagucac cgucgaaguc caauacgcag gcaccgacgg accaugcaaa guacccgcac     1140

aaauggccgu agacaugcaa acacuaaccc cagugggacg acucauaacu gccaacccag     1200

ucaucaccga aucaaccgaa aacagcaaaa ugaugcuaga gcucgaccca ccauucggag     1260

acagcuacau agucaucggc gucggcgaga agaaaauuac gcaucacugg cacagaagcg     1320

gcucgaccau aggcaaagcc uucgaagcaa caguacgcgg agcaaaacga auggccgucc     1380

ucggagacac agccugggac uucggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7412
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 2 opt

<400>  7412
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaagucau cuaccuagua augauccugc uaauagcacc agccuacagu aucagaugca      120

uaggagucuc gaacagggac uucguagagg gaaugucagg gggcacgugg guagacgugg      180

uacuagaaca cggcggaugc gugaccguga uggcgcaaga caaaccgacc guagacauag      240

aacucgucac gacaacgguc ucaaacaugg ccgaggucag gagcuacugc uacgaagcga      300

guauaagcga cauggcaagc gacagcagau gcccgacaca gggcgaggcc uaccucgaca      360

aacagagcga cacccaauac gugugcaaac gcacccuagu ggaccgaggc uggggcaacg      420

gcuguggccu guucgggaag gggagccucg ucacaugcgc aaaauucgcc ugcagcaaga      480

aaaugacagg aaaaagcauc cagcccgaaa accuggaaua cagaauaaug uuaagcgugc      540

acggaagcca gcacucaggc augaucguaa acgacacagg ccacgagacc gacgaaaacc      600

gcgcaaaagu ggaaauaacc ccgaacucgc cgagggcaga agccgcccua gggggauucg      660

gcucgcuagg gcuagacugc gagccaagaa caggccugga cuucagcgac cuauacuacc      720

uaacgaugaa caacaaacac uggcuagugc acaaggagug guuccacgac auacccuugc      780

ccuggcacgc gggagccgac acaggaacgc cucacuggaa uaacaaggaa gcccuagucg      840

aguucaaaga cgcacacgca aaacgacaga ccguaguagu gcuagggagc caggaagggg      900

cagugcacac ggcucuggcc ggagcacugg aagcggaaau ggacggggcc aaaggcaggc      960

ucucauccgg gcaccugaaa ugccgccuga agauggacaa acuaagacuc aaaggcguca     1020

gcuauagccu augcacggcc gcguucacgu ucacgaaaau accagccgaa acgcuacacg     1080

gaacggucac gguagaaguc caguacgcgg gcacggacgg gccaugcaaa gugccggcgc     1140

aaauggccgu agacaugcaa acgcucacgc cagugggaag gcuaauaacc gcgaacccag     1200

uaauaacaga aagcacagaa aauagcaaga ugaugcucga gcucgauccc ccguucgggg     1260

acucauacau agucauaggc gugggcgaga aaaagauaac gcaccacugg cacagaucgg     1320

gaucgaccau aggcaaagcc uuugaagcga ccguacgcgg ggcaaaacgc auggcaguac     1380

ucggggacac ggcaugggac uuugguucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7413
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 2 opt

<400>  7413
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaagucau auaccucgua augauccuac uaaucgcacc agcauacagc auaagaugca      120

uaggcguuuc caaccgagac uucguagaag gcaugagcgg cggcacaugg guagacgucg      180

uccucgaaca cggaggaugc guaaccguca uggcccaaga caaacccaca gucgacaucg      240

aacuggucuc uacaacaguc uccaacaugg ccgaaguccg aagcuacugc uacgaagcaa      300

ccauaucgga caucgcaucc gacagccgcu gcccgacaca aggcgaagcc uaccucgaca      360

agcaaagcga cacacaauac gucugcaaac gaacgcucgu cgaccgaggc ugggguaacg      420

gcugcggccu cuucggcaaa gggucccucg uaacaugcgc caaauucgcc ugcucaaaga      480

aaaugacagg caaauccauc caaccagaaa accucgaaua cagaaucaug cuauccguac      540

acggcagcca acacuccggc augauuguaa acgacacagg ccacgaaacc gacgaaaacc      600

gagcaaaagu agaaaucacc ccaaacagcc cccgagcaga agccacccuc ggcggauucg      660

gaagccucgg ccucgacugc gaacccagaa ccggacucga cuucagcgac cucuacuacc      720

ucaccaugaa uaacaaacac uggcucguac acaaagaaug guuccacgac aucccccuac      780

ccuggcacgc aggcgccgac accggaacac cccacuggaa caacaaagaa gcacucgucg      840

aguucaaaga cgcccacgcc aaacgccaga ccgcagucgu ccucggauca caggaaggcg      900

ccguccauac agcccuagcc ggcgcccuag aagcagaaau ggacggcgcg aaaggccgcc      960

ucagcagcgg ccaccuaaaa ugccgccuca aaauggacaa acuacgccuu aaaggcguca     1020

gcuauagccu cugcacagcu gcauucaccu ucacaaaaau ccccgcagaa acccuacacg     1080

gcacagucac cgucgaagua caauacgccg gcacagaugg cccaugcaaa gugcccgccc     1140

aaauggccgu ggacaugcaa acccucaccc cagucggcag acucauaacc gccaacccag     1200

uaaucaccga aucaaccgaa aacuccaaaa ugaugcucga acucgauccc ccauuuggag     1260

acuccuacau agucaucggc guaggcgaaa agaaaaucac ccaccacugg caccgcagcg     1320

gauccaccau aggcaaagcc uucgaagcca caguccgagg agcaaaaaga auggccgugc     1380

ucggagacac agcaugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7414
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 2 opt

<400>  7414
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaagucau auaccuaguc augauccuac uaauagcacc agcguauagc auacgcugca      120

ucggaguaag caacagggac uucgucgagg gcaugagcgg aggcacaugg guggacgucg      180

uacuggaaca cggaggaugc guaacaguga uggcacaaga caaaccaacc guagacauag      240

agcuaguaac aacaacaguc ucgaacaugg ccgaagucag aucauacugc uacgaagcca      300

gcauaagcga cauggcaucc gacuccaggu gcccaaccca gggcgaagca uaccucgaca      360

aacaaagcga cacacaguac guaugcaaaa gaacccuagu ggacagagga uggggcaacg      420

gaugcggacu auucggaaaa gggagccucg ucacaugcgc aaaauucgcc ugcagcaaga      480

aaaugacagg gaaaucaaua cagccagaaa accuagaaua ccgaauaaug cucagcgugc      540

acggaagcca acacucaggc augaucguaa acgacaccgg gcacgaaaca gacgaaaacc      600

gcgcaaaagu cgaaauaaca ccaaacucac ccagagccga ggccacacuu gggggauucg      660

gcagccucgg uuuagacugc gaaccaagga ccgggcucga cuucucagac cuguacuacc      720

ucacaaugaa uaacaaacac uggcucguac acaaagaaug guuccaugac aucccccuac      780

ccuggcacgc cggcgcagac acaggcacgc cacacuggaa caacaaagaa gcgcucgugg      840

aguucaaaga cgcccacgcc aaacgccaaa ccguaguggu gcuagggagc caggaaggag      900

cagugcacac agccuuagcc ggcgcccugg aagcagaaau ggacggagca aaaggccggc      960

ucagcagcgg acaccucaaa ugccgacuga aaauggacaa acuacgccua aaaggaguga     1020

gcuacagccu cugcacagcc gcguucacau ucagcaagau acccgcggaa acacuacacg     1080

gaacaguaac agaagaaguc caauacgcag gcaccgacgg acccugcaag gugcccgcac     1140

aaauggccgu agacaugcaa acacucacac cgguuggcag acucaucacc gcaaaccccg     1200

ugauaaccga aagcacagaa aacucaaaaa ugaugcucga acucgacccg ccauucggag     1260

acucguacau aguaauagga guaggcgaaa agaaaaucac gcaccacugg caccgcagcg     1320

gaucaacaau cggcaaagcc uucgaagcga ccgucagagg agcaaaacgc auggcgguac     1380

ugggagacac agcaugggac uucggaucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7415
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 2 opt

<400>  7415
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

aaaaaguaau auaccuagua augauccucc uaauagcacc cgcauacagc auccgcugca      120

uaggggucuc gaacagggac uucguagaag gcaugagcgg aggaacaugg guagacguag      180

ucuuagaaca cggcggcugc gucaccguaa uggcccaaga caaaccaacc guagacauag      240

aacuggucac caccacggua ucgaacaugg cagaaguccg aagcuacugc uacgaagcca      300

gcaucagcga cauggccuca gacagccgau gccccaccca aggcgaagcc uaccucgaca      360

aacaaucaga cacccaauac guaugcaaac gcacccuagu agaccgaggc uggggcaacg      420

gcugcggccu guucggaaaa gggagccugg ucacaugcgc caaauucgcc ugcuccaaga      480

aaaugacagg aaaaucaauc caacccgaaa accuagaaua ucgcaucaug cuauccguac      540

acggauccca acacuccgga augauaguaa acgacacagg acacgaaacc gacgaaaacc      600

gcgccaaagu agaaauaacc ccaaacucuc ccagagccga agccacacuc ggaggauucg      660

gcucccuagg ccuagacugc gaaccccgga ccgggcucga cuucuccgac cucuacuacc      720

ucaccaugaa caacaaacac uggcucgucc auaaagaaug guuucacgac auaccccucc      780

cauggcacgc aggcgcagac accgguacac cacacuggaa caacaaggaa gcgcuaguag      840

aguucaaaga cgcccacgcc aaacgccaaa ccguagucgu gcuaggcagc caggaaggag      900

ccguacacac agcacuggcc ggagcucucg aagcagaaau ggacggagcc aaaggccggc      960

uaucaagcgg acaccuaaaa ugccgccuaa aaauggacaa gcuaagacuc aaaggcguga     1020

gcuacagccu augcacagcc gcauucacgu ucaccaaaau accugcagaa acccuacacg     1080

gaacaguaac agucgaacuc caauacgcag gcacggacgg acccugcaaa gugcccgccc     1140

aaauggccgu ggacaugcaa acgcucacac ccguaggaag gcucauuaca gcaaacccag     1200

ucaucaccga aagcacagaa aacucaaaaa ugaugcuaga acuugauccc ccauucggag     1260

acucguacau agucaucggc guaggggaga aaaagaucac acaccacugg caccgaucag     1320

gcucaacaau cggcaaggcc uucgaagcca ccguccgagg agcaaaacga auggccguac     1380

uaggggacac agccugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7416
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 2 opt

<400>  7416
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau auaccugguc augauccucc uaauugcacc agccuacagc auacguugca      120

ucggcaucuc caacagagac uuugucgaag gcaugagcgg agggacgugg guagacgugg      180

uacuagaaca cggaggcugu guaaccguaa uggcccaaga caaaccaacc gucgacaucg      240

agcuagucac uacaacaguc agcaacaugg cagaaguccg aagcuacugc uacgaagcca      300

gcauaagcga uauggcgagc gacagcagau gcccaaccca aggugaagca uaccuagaca      360

aacaaagcga cacccaguac gugugcaaac gcacacuggu agaccggggc uggggcaacg      420

gcugcggccu guucggcaaa ggaucccuag ucacaugcgc aaaauucgcc ugcagcaaga      480

aaaugaccgg aaaaucuauc caaccagaaa accuagaaua ucgcauaaug cuaagcguac      540

acggaagcca gcacuccgga augaucguaa acgacaccgg acacgaaacc gacgaaaacc      600

gagccaaagu agaaaucacc cccaacucgc ccagagcaga agcaacccuu ggaggcuucg      660

gaauccuagg acucgacugc gaacccagga ccggacuaga cuucagugac cuguacuacc      720

uaacaaugaa caacaaacac uggcucgucc acaaggaaug guuccacgac auaccccucc      780

cuuggcacgc aggcgcagac acaggaacgc cacacuggaa caacaaggaa gcacucgucg      840

aguucaaaga cgcccacgcc aaacgucaaa ccgucguagu ccucggcuca caagaaggag      900

ccguacacac agcccuggca ggagcccucg aagcagaaau ggacggagca aaaggacgcc      960

uaagcagcgg gcaccuaaaa ugccgccuaa aaauggacaa acuccgacuc aaaggcguaa     1020

gcuacagccu augcaccgcc gcauucaccu ucacaaaaau acccgcagaa acccuccacg     1080

gcaccgucac cgucgaaguc caauacgcag gaaccgacgg accaugcaaa guccccgcac     1140

aaauggcagu agacaugcaa acccucacac caguaggaag acucaucacc gcaaacccag     1200

ucaucaccga gagcaccgag aacucaaaaa ugaugcucga acucgaccca cccuucggcg     1260

acucuuauau agucaucggg guaggagaaa agaaaaucac acaccacugg caccgaucgg     1320

gcuccaccau aggaaaagcc uucgaagcaa ccguccgcgg agccaaacgc auggcugugc     1380

ucggagacac ggcuugggac uucggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7417
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 2 opt

<400>  7417
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau auaccucguc augauacuac uaauagcccc ggcauacagu aucagaugca      120

ucggggucag caacagagac uucgucgaag gcaugagugg aggcacgugg guagacguag      180

uacuagaaca cggaggaugc gucacaguca uggcacaaga caaaccgacc guagacauag      240

agcuaguaac aaccaccguc agcaacaugg ccgaagucag aagcuacugc uacgaagcca      300

guauauccga cauggccagc gacucgaggu gccccaccca aggcgaagcc uaccuugaca      360

aacaaagcga cacacaauac guaugcaaaa gaacccuagu cgaccgaggc uggggaaacg      420

gaugcggccu cuucggaaag ggaagccuag ucacaugcgc caaauuugca ugcagcaaga      480

aaaugacagg aaaaagcaua caacccgaaa accuagaaua ccgcaucaug cuaagcgucc      540

acggaagcca gcauagcggc augauaguaa acgacacagg gcacgaaacc gacgaaaacc      600

gagccaaagu agaaaucaca ccaaacagcc ccagagccga agccacgcuc ggaggcuucg      660

gaagccuagg acucgacugc gaacccagaa ccggacucga cuucagugac cucuacuacc      720

uaacaaugaa uaacaaacac uggcuggucc acaaagaaug guuccacgac aucccacuac      780

ccuggcacgc aggagcggac acgggcacac cacacuggaa caacaaagaa gcucucguag      840

aauuuaaaga cgcccacgca aaacgccaga ccguagucgu ccuaggaagc caagaaggag      900

ccguacacac cgcccuggca ggagcgcugg aagcggaaau ggacggagua aaaggaaggc      960

uaagcagcgg ccaccucaaa ugccgccuaa aaauggacaa acuacgccua aagggcguaa     1020

gcuacagccu augcacagcc gcguucacau ucacaaagau accagccgaa acgcuccacg     1080

ggacagucac aguagaaguc caauacgcag gaacagacgg accaugcaaa gugccagcac     1140

aaauggccgu cgacaugcaa acccuaaccc ccguaggccg gcuaauaaca gcaaacccug     1200

ucauaaccga aucgacagaa aacuccaaga ugaugcuaga acucgauccc ccauucggcg     1260

acaguuacau ugucauagga gugggcgaaa agaaaauaac acaccacugg caucgaagcg     1320

gaucaaccau cggcaaagcc uucgaagcca ccguacgagg agccaaacgc auggcagugc     1380

uaggcgacac agcaugggac uucggcaguu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7418
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 2 opt

<400>  7418
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau auaccuaguc augauccuac ucauagcccc agcauacagc auacgcugca      120

uaggaguguc caaccgagac uucgucgaag gcaugucagg cggcacgugg guagacguag      180

uacuagaaca cggcgggugc guaacaguga uggcccaaga caaacccaca gucgacaucg      240

agcucgucac aaccacaguc uccaacaugg ccgaagucag aucauauugc uacgaagcaa      300

gcaucuccga cauggcaagc gacagcagau gcccaaccca aggagaagcc uaccuugaca      360

aacaaucaga cacccaauac gucugcaaac gcacccuagu cgaccgggga uggggcaacg      420

gaugcgggcu auucggaaaa ggaucccuag uuacaugugc caaauucgca ugcucaaaga      480

aaaugaccgg gaaaucaaua caacccgaaa accuagaaua ccgcaucaug cugagcguac      540

acggcagcca acauucaggc augaucguaa acgacaccgg acacgaaaca gaugaaaacc      600

gcgccaaagu ggaaauaacu ccaaacuccc ccagagccga agccacacuc ggaggcuucg      660

gcucgcucgg acucgacugc gaacccagaa caggccuuga cuucucagac cuauauuacc      720

uuacaaugaa caacaaacac uggcucguac acaaagaaug guuccacgau auaccccuac      780

ccuggcacgc aggcgcagac accggcacac cccacuggaa caacaaagaa gcccuaguag      840

aguucaaaga cgcacacgca aaacgccaaa ccgucguagu ccucggauca caagaaggcg      900

ccguacacac ggcccuugcc ggcgcacuag aagcagagau ggacggcgcc aaaggccgac      960

uaucaagcgg gcaccugaaa ugcagacuca aaauggacaa acuccgacua aagggcgugu     1020

ccuacucacu augcacagca gcauucacau ucacaaagau acccgcagaa acccuacacg     1080

gaacgguaac agucgaagua caauacgccg gcgcagaugg accaugcaaa guacccgcgc     1140

aaauggccgu agacaugcaa acacucacgc ccgucggacg acucauaacu gcgaaccccg     1200

ucauaacaga aagcacagaa aacuccaaaa ugaugcuaga acucgauccc ccauucggcg     1260

acuccuauau agucaucggc gucggagaga agaaaauaac acaccacugg caccgcuccg     1320

gcuccacuau cggcaaagcc uucgaagcca ccguacgagg agccaaacga auggccgucc     1380

ucggagacac cgcaugggac uuuggaucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7419
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 2 opt

<400>  7419
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

agaaagucau cuaccuggua augauccuac uaauagcccc cgcauacagc auaagaugca      120

ucggaguauc caacagagac uucgucgaag gaauguccgg cggcacaugg guagacguag      180

ucuuggaaca cggaggcugc guaaccguca uggcacaaga caaacccacc gucgacaucg      240

aacucgucac gaccacaguc ucgaauaugg ccgaagucag aagcuacugc uacgaagcaa      300

gcauaagcga cauggccagc gacagcagau gcccaaccca aggcgaagcc uacuuagaca      360

aacaguccga cacccaauac guaugcaaac gcacccucgu agaccgaggc uggggaaacg      420

gcugcggccu guucggaaaa ggaagccucg uaaccugcgc gaaauucgcc ugcuccaaga      480

aaaugaccgg aaaaucaauc cagccugaaa accucgaaua ccgcauaaug cucagcgucc      540

acggaagcca gcauagcggc augaucguaa augacacagg acacgaaacc gacgaaaacc      600

gcgcaaaagu agaaauaaca ccgaacuccc ccagggccga ggccacacuc ggcggcuucg      660

gcucgcucgg ccucgacugc gaaccaagga ccggacuaga cuucagcgac cuauacuacc      720

ucacaaugaa caacaaacac uggcuaguac acaaagaaug guuccacgac auaccccucc      780

cauggcacac aggcgccgac accggcacac cccacuggaa caacaaagaa gcccuaguag      840

aguucaaaga cgcccaugcc aaacgccaaa ccguggucgu ccucggauca caggaaggcg      900

ccguucacac cgcucuagca ggagcccuag aagccgaaau ggacggagcc aagggacgac      960

ucucaagcgg acaccucaaa ugcagacuca aaauggacaa gcuacgacua aaaggaguaa     1020

gcuauagccu gugcaccgca gcauucaccu ucaccaaaau acccgcagaa acacuccacg     1080

gcacgguaac cgucgaagua caauacgcag gcaccgacgg cccaugcaaa guacccgccc     1140

aaauggccgu cgacaugcaa acacuaaccc ccguaggccg acucauaaca gcaaacccag     1200

ucauaaccga aggcaccgaa aauagcaaga ugaugcucga acuggacccg cccuucggcg     1260

acucguacau agucauagga gugggcgaga aaaagaucac acaccacugg caccgcuccg     1320

gcucaaccau aggcaaagcc uucgaagcca ccguccgagg ggccaaacgc auggccgugc     1380

ucggagacac agccugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7420
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 2 opt

<400>  7420
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaaguaau auaccucguc augauccucc ucauagcccc cgccuacucc auacgcugca      120

uaggcgucuc caaccgggac uucgucgaag gaaugagugg gggcaccugg gucgacguag      180

uccuagaaca cggcggaugc guaaccgcaa uggcccagga caagcccacc gucgacauag      240

aacucguaac gacaacaguc uccaacaugg ccgaagucag auccuacugc uacgaagcaa      300

gcaucucaga cauggcaagc gacagccgau gcccaaccca aggcgaagcc uaccuagaca      360

aacaaagcga cacccaauac guaugcaaac gaacccuagu cgaccgcggc uggggcaacg      420

ggugcggccu auuugggaag ggaagccucg ucacaugcgc aaaauuugca ugcagcaaaa      480

agaugaccgg gaaaagcauc caaccagaaa aucucgaaua ccgcauaaug cuauccgucc      540

acggcagcca acacagcggc augcuaguaa acgacaccgg acacgaaacc gacgaaaacc      600

gcgcaaaagu agaaauaaca ccaaacuccc cacgagcaga agcaacacuc ggcggcuucg      660

gaagccuagg ccuagacugc gaacccagaa ccggacucga cuucagcgac cucuacuacc      720

ucacaaugaa caacaaacac uggcuagccc acaaggaaug guuccacgac aucccacuac      780

ccuggcacgc aggcgcagca acgggaacac cacacuggaa caacaaagaa gcccuaguag      840

aguucaaaga cgcacaugcu aaacgacaaa ccgucguagu acuaggaagc caagaaggcg      900

caguccacac cgcccucgcc ggagcacuag aagccgaaau ggacggagcc aaggggagac      960

uuucaagcgg ccaccuaaaa ugucgccuaa aaauggacaa acuacgccuc aaaggcguca     1020

gcuacagccu cugcacagcc gcauucacau ucaccaaaau accugcugaa acagucgacg     1080

gaacaguaac agucgaagga caauacggag gaacugacgg ccccugcaag guaccagcac     1140

aaauggccgu cgacaugcag acgcuaacgc caguaggacg acucauaacc gccaacccag     1200

ucauaaccga auccacagaa aacagcaaaa ugaugcucga acuagaccca cccuucgggg     1260

acuccuacau agucauagga gucggcgaaa agaaaaucac ccaccacugg caccgcagcg     1320

gcucaaccau aggcaaagca uucgaagcga caguacgcgg agcaaaacgc auggccguac     1380

uaggagacac agccugggac uucggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7421
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 2 opt

<400>  7421
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

aaaaggugau auaccuaguc augauccucc uaauagcccc agccuacagc auacggugca      120

ucggagucuc caacagagac uucgucgaag gcaugagcgg aggaacaugg guagacguag      180

uccuagaaca cggcggaugc guaacaguga uggcgcaaga caaaccagcc guagacauag      240

aacuggucac aacaacgguc agcaacaugg cagaagucag aagcuacugc uacgaagcaa      300

gcauaagcga cauggcgagc gacagcagau gcccaaccca gggcgaagca uaucucgaca      360

aacaaucaga cacacaguac guaugcaaga gaacgcucgu agaccgaggc uggggcaacg      420

gaugcggccu guucggaaag gggagccucg ucacgugcgc gaaauucgcg ugcagcaaga      480

aaaugaccgg gaaaucaaua cagccagaga accuggaaua ccgaauaaug cucagcgucc      540

acggcagcca acacagcggc augaucguca acgacacagg acaugaaacc gacgaaaauc      600

gcgcaaaagu cgaaaucaca ccgaacucgc ccagagccga agccacacuc gggggauucg      660

guagccuagg acucgacugc gagcccagga caggacucga cuucucggac cuguacuacc      720

uaacaaugaa caacaaacac uggcucgugc acaaagaaug guuccacgac auaccccuac      780

ccuggcacgc aggcgcagac acaggcacac cccacuggaa caacaaagaa gcccuagugg      840

aguucaaaga cgcacaugca aaacgacaaa ccguaguagu gcuaggaagc caggaaggag      900

ccguacacac agcccuggcc ggagcauugg aagccgaaau ggacggagca aaaggcaggc      960

ucuccagcgg gcaccugaaa ugccgccuga aaauggacaa acuacgacuc aaaggcgugu     1020

ccuacagucu cugcaccgca gcguucacau ucacaaaaau acccgcagaa acgcuccacg     1080

gcacggucac ggucgaaguc caauacgcug gaaccgacgg acccugcaaa guaccagcgc     1140

aaauggccgu ggacaugcag acgcucacac cagugggacg gcucauaaca gcgaacccag     1200

ucauaacaga aagcacagaa aacaguaaaa ugaugcucga acuagacccg cccuucgggg     1260

acuccuacau aguaaucggc guaggcgaaa agaaaauaac gcaccacugg caccgcuccg     1320

gcaguaccau aggaaaagca uucgaagcga caguacgagg agccaagcgc auggcgguac     1380

ugggagacac agccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7422
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 2 opt

<400>  7422
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaagucau cuaccuugua augauccucc uaauagcccc agcguacagc auccgcugca      120

ucggcguaaa caacagggac uucguagaag gcauguccgg aggcaccugg guagacgucg      180

uacucgaaca cgguggaugc guaaccguaa uggcccaaga caaaccaacc guagacaucg      240

agcuagucac gaccacagua agcaacaugg cagaagucag gagcuacugc uacgaagcaa      300

gcaucagcga cauggcaagc gacagccgcu gcccaaccca aggagaagcg uaccucgaca      360

aacaaagcga cacacaauac gucugcaaaa gaacccucgu ggaccgaggc uggggcaacg      420

gcuguggccu auucggcaaa gggagccuag ucacaugcgc caaauucgca ugcagcaaga      480

aaaugaccgg aaaaagcauc caacccgaaa accuagaaua ccgaauaaug cugagcgucc      540

acggaagcca acacucaggc augaucguca acgacaccgg ccacgaaaca gacgaaaaca      600

gagcaaaagu agaaaucacc ccaaacagcc ccagagccga agcaacacua ggcggcuucg      660

gaagccuggg acuggacugc gagccccgaa ccggacuaga cuucucagac cuauacuacc      720

ucacaaugaa uaacaaacac uggcuaguac acaaagaaug guuccacgac auaccacugc      780

cauggcacgc cggcgcggac accggaacac cccacuggaa caacaaagaa gcacucguag      840

aguucaaaga cgcccacgcc aaacgccaaa ccgucguagu acuaggcagc caagaaggag      900

ccguacacac agcccuggcc ggagcacucg aagccgaaau ggacggggcc aaaggccgac      960

ucuccagcgg gcaccucaaa ugccgccuca aaauggacaa acuacgacuc aaaggcguaa     1020

gcuacagccu cugcacagcc gcauucacau ucaccaaaau acccgccgaa acccuacacg     1080

guacagugac agucgaagua caauacgcag gcacggacgg gcccugcaaa guacccgcac     1140

agauggccgu agacaugcaa acacuaacac cagucggcag acucaucaca gccaaccccg     1200

uuaucaccga aagcacagaa aacagcaaaa ugaugcucga acuagaccca ccauuuggcg     1260

acagcuacau agucauugga gucggagaaa agaaaaucac acaccacugg cacagauccg     1320

gcagcacaau cggaaaagcc uucgaagcaa ccguccgagg cgcgaaacga auggcaguac     1380

uaggagacac agcaugggac uucgguagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7423
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 2 opt

<400>  7423
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

aaaaaguaau auaccuagua augauauuac ucauagcacc ggccuacagc auccgcugua      120

uagggguauc caacagagac uucgucgagg gcaugagcgg cggcacaugg gucgacgucg      180

uacuagagca cggcggcugc guaacaguca uggcacaaga caaaccaacc guggacaucg      240

agcuagucac caccacaguc ucaaacaugg ccgaagucag aagcuacugc uacgaagcga      300

gcaucagcga cauggcaagc gacagcagau gcccaaccca aggcgaggcu uaccucgaca      360

agcagagcga cacccaauac gugugcaaac gcacgcuagu agacagaggc uggggaaaug      420

gaugcggacu uuucggaaaa ggaagccuag uuacgugcgc aaaauucgcc ugcagcaaga      480

aaaugaccgg gaaaucaauc caacccgaaa accuagaaua ccgaauaaug cuaagcguac      540

acggcagcca acacucggga augauaguaa acgacacagg acacgagaca gacgaaaaca      600

gagccaaagu agaaauaaca ccgaacucac ccagggccga ggcaacacuc ggaggcuucg      660

gcucgcuagg gcucgacugc gagcccagaa ccggccuaga cuucuccgac cuauacuacc      720

ucacaaugaa uaacaaacac uggcuaguac acaaggaaug guuccacgac auaccacugc      780

ccuggcacgc aggcgcagac acaggaacac cccacuggaa caacaaggaa gcccuagucg      840

aguucaaaga cgcacacgca aagcgacaaa ccguagucgu ccuuggaagc caagaaggag      900

ccguacacac agcccucgca ggggcccugg aagcagaaau ggacggagcc aaaggcagac      960

uauccagcgg acaccugaag ugccgacuca aaauggacaa gcucaggcuc aagggagucu     1020

ccuacagccu augcgcagcc gccuucacau ucacaaaaau acccgcggaa acgcuccacg     1080

gcacaguaac ggucgaaguc caguacgcag gcacagacgg acccuguaaa guacccgcgc     1140

aaauggcagu cgacaugcaa acacuaacac cagucggcag gcucauaacc gccaacccgg     1200

ucauaaccga gaguacagaa aacagcaaaa ugaugcucga acucgauccc ccauucggag     1260

acagcuacau aguaauagga guaggcgaaa agaaaaucac ccaccacugg cacagaucag     1320

gcucaacaau aggcaaagca uucgaagcaa ccguacgcgg agcaaaacga auggccgucc     1380

uaggggacac cgccugggac uuugguucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7424
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 2 opt

<400>  7424
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaaaguaau cuaccucgua augauccuac uaaucgcacc cgccuacagc auacgaugca      120

ucggaguauc aaaccgcgac uucgucgaag guauguccgg cggaacaugg guagacgucg      180

ucuuagaaca cggagggugc guaacaguca uggcccaaga caaaccaaca guagacauag      240

agcucguaac aaccaccgua agcaacaugg cagaaguccg cagcuacugc uacgaggcca      300

gcauaucaga cauggccucc gacucacgau gcccaacaca aggagaagca uaccuagaca      360

aacaaagcga cacccaauac gucugcaaaa gaacacuggu cgacagagga uggggcaacg      420

gaugcggccu auucggaaaa ggcagccuag uaaccugcgc aaaauucgcc ugcagcaaga      480

aaaugaccgg caaauccaua caaccagaaa aucuagaaua ccgaauaaug cuauccgucc      540

acggaagcca acacuccggc augaucguca acgacaucgg ccacgaaacc gacgaaaacc      600

gagccaaagu cgaaaucacc cccaacucac cccgagcaga agccacacuc ggcggcuucg      660

gaucccucgg acuagacugc gaacccagaa caggccucga cuucagcgac cucuacuacc      720

uaacaaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac auaccacugc      780

ccuggcacgc aggcgccgac acaggcacgc cccacuggaa caacaaagag gcacucgugg      840

aguucaaaga cgcccacgcc aaaagacaaa caguaguagu acucggcagc caagaaggag      900

ccguccacac agcccucgca ggagcccucg aagccgaaau ggacggagcc aaaggcagac      960

ucuccagcgg gcaccugaaa ugccgccuca aaauggacaa acuacgccuc aaaggcguca     1020

gcuacagccu cugcacagca gccuucaccu ucaccaaaau ccccgccgaa acacuccacg     1080

gaaccguaac aguagaaguc caauacgccg gcacagacgg acccugcaaa guacccgcac     1140

aaauggccgu cgacaugcag acccucacgc ccguaggaag acucaucacc gcaaaccccg     1200

ucauaaccga aagcacagaa aacagcaaga ugaugcuaga acucgaccca cccuucggag     1260

acagcuacau cguaauagga gugggagaca agaaaaucac acaucacugg caccgcagcg     1320

gcucaacaau aggcaaagca uuugaagcca caguacgcgg ggccaaaaga auggccgucc     1380

uaggggacac cgccugggac uucggauccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7425
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 2 opt

<400>  7425
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaguc       60

aaaaagucau auaccucguc augauccugc uaaucgcccc cgccuacagc auacgcugua      120

ucggagugag caacagggac uucguagaag gaaugagugg aggcaccugg gucgacguag      180

uacuagaaca cggaggaugc guaaccguaa uggcacaaga caaaccaacc guagacaucg      240

agcuagucac aaccacaguc ucaaacaugg ccgaagucag aagcuacugc uacgaagcaa      300

gcaucagcga cauggcaagc gacagccgau gcccaacaca aggagaagca uaccuagaca      360

aacaaagcga cacccaauac gugugcaaac gcacccuagu cgaccgagga uggggcaaug      420

gaugcggccu cuucggaaaa ggaucccuag ucacaugcgc gaaauucgca ugcagcaaga      480

aaaugacugg aaaaucaauc cagccagaaa accuagaaua ccgaaucaug cucucgguac      540

acggcaguca acacagcggu augauaguga acgacaccgg acacgaaacc gaugaaaaca      600

gagccaaagu cgaaauaacc ccaaacuccc ccagagccga agccacacua ggaggcuucg      660

gaucgcuagg acuugacugc gaaccaagaa ccggacuaga cuucuccgac cuauacuauc      720

uaacaaugaa caacaaacac uggcucgugc acaaagaaug guuccacgau auaccacugc      780

cauggcacgc aggagcggac acaggaacac cccacuggaa caacaaagaa gcccuaguag      840

aguucaaaga cgcccacgcc aaacgacaaa ccgucgucgu acuaggcagc caggaaggag      900

ccguacacac agcccucgcg ggcgcccuag aagccgaaau ggacggagca aaaggcagac      960

uaucgucagg ccaccucaaa ugccgccuga aaauggacaa acuaaggcuc aaaggagugu     1020

cauacagccu cugcacagcc gcguucacau ucacaaaaau accagcagaa acacuccacg     1080

gaacaguaac aguagaaguc caauacgcag gaacagacgg acccugcaaa guccccgccc     1140

aaauggccgu agacaugcaa acccucacgc cagucggcag acugauaacc gcaaacccag     1200

uaaucacaga aagcacagaa aacucaaaaa ugaugcucga acuagaccca ccauucggcg     1260

acagcuauau aguaauaggc gucggagaca agaaaaucac acaccacugg uaccgcucag     1320

gcuccaccau cggaaaagcc uucgaagcca ccguacgagg agcaaaacgc auggcagugc     1380

uaggggacac ggccugggac uucggaagcu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7426
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 2 opt

<400>  7426
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaaaaguaau auaccuagua augauccuac uaauagcccc cgcauacagc auccgcugca      120

uagggguauc caacagggac uucgucgaag gcaugagcgg aggcacaugg gucgacgugg      180

uccuagagca cggaggcugc guaaccguaa uggcacaaga caaaccaacc gucgacaucg      240

aacuggucac caccacaguu agcaacaugg ccgaagucag aagcuacugc uacgaagcaa      300

gcaucucaga cauggcaagc gacucccggu gccccacaca aggagaagca uaccuagaca      360

aacagagcga cacccaauac guaugcaaac gcacacucgu cgaccgaggc uggggcaacg      420

gcugcggacu auucggaaaa ggaagccucg ucacaugcgc aaaauucaca ugcagcaaaa      480

aaaugacagg aaagagcauc caaccagaaa accuagaaua ccgcauaaug cuaagcguac      540

acgguagcca acacucaggc augauaguca acgacaccgg ccacgaaacc gacgaaaacc      600

gagccaaagu agaggucacc cccaacagcc cacgagccga agcaacccua ggcggcuucg      660

gcagcuuagg ccucgacugc gaaccccgca ccgggcuaga cuucagcgac cuauacuacc      720

uaaccaugaa caacaaacac uggcucgugc acaaagaaug guuccacgac auacccuuac      780

cauggcacgc cggcgcggac acaggcacac cccacuggaa caacaaagaa gcccucgucg      840

aauucaaaga cgcucacgcc aaacgccaaa caguaguagu acuaggcagc caggaaggcg      900

cgguacacac cgcccuagcc ggagcccuag aagccgaaau ggacggggcc aaaggccgac      960

ucuucagcgg acaccugaaa ugccggcuga aaauggacaa acucagacua aaaggcguca     1020

gcuacagccu augcacagca gccuucacau uuacaaaagu acccgcagaa acacuccacg     1080

gcaccgucac agucgaagua caauacgcag gcacagacgg cccaugcaaa guaccagcac     1140

aaauggccgu agacaugcaa acacucacac cagugggcag acucaucacc gccaacccag     1200

uaauaaccga aagcaccgaa aacuccaaaa ugaugcuaga acucgaccca ccauucggcg     1260

acuccuacau aguaauaggc gucggcgaca aaaaaaucac acaccacugg caucgaagcg     1320

gcuccacaau cggcaaagca uucgaagcaa ccguaagagg agccaaacga auggcuguac     1380

ucggcgacac cgcaugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7427
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 2 opt

<400>  7427
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaagucau cuaccuagua augauccucc ucauagcccc agcuuacagc aucagaugua      120

ucggaguauc caacagagac uucgucgaag gcaugagcgg gggcacaugg gucgacguag      180

uccuagagca cggcgggugu gucacuguaa uggcgcaaga caaaccaaca guagacauag      240

agcuaguaac aaccaccguc ucaaacaugg ccgaaguccg aagcuacugc uaugaagcca      300

gcauaagcga cauggcaagc gacagcagau gcccgacaca aggcgaagcc uaccuagaca      360

aacaaagcga cacccaauac guaugcaaac gcacccuagu agaccgggga uggggaaacg      420

gcugcggccu auucgggaaa ggaagccuag uaacaugcgc caaauucaca ugcagcaaga      480

aaaugaccgg gaaaucaauc caacccgaaa accuagaaua ccgcauaaug cugagcgugc      540

acggaagcca acacucaggc augauaguaa acgacaucgg ccacgaaaca gacgaaaacc      600

gagcgaaagu agaagugaca ccgaacucac ccagggcaga agccacgcuc ggaggcuucg      660

guucguuagg acuagacugc gagcccagaa caggacucga cuuuucggac cuguacuacc      720

uaaccaugaa caacaaacac uggcugguac acaaagaaug guuccacgac auaccccugc      780

cauggcacgc aggagcagac acuggcacac cccacuggaa caacaaagaa gcacucgugg      840

aguucaaaga cgcccacgcc aaacgacaaa ccgucgucgu gcucggaagu caagaaggag      900

cagugcacac cgcccucgcc ggagcacuag aagcggaaau ggacggggcc aaaggccgcc      960

ucuucagcgg acaccucaaa ugcaggcuga aaauggacaa acuaagacuc aagggaguaa     1020

gcuacagccu cugcaccgcu gcauucaccu ucacgaaagu acccgcggaa acgcuacacg     1080

gaacaguuac ggucgaaguc caguacgcag gcacggacgg gcccugcaaa gugcccgcac     1140

agauggccgu ugacaugcaa acacucaccc cagucggcag acucauaacu gcaaacccgg     1200

ucaucaccga aaguacggaa aacagcaaga ugaugcuaga gcuagauccc ccauucgggg     1260

acuccuacau agucaucggc gugggcgaca agaaaaucac gcaccacugg caccgaucgg     1320

gcucgaccau cggcaaagcc uuugaagcaa ccgugcgagg agccaaacgc auggccgucc     1380

ucggcgacac agccugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7428
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 2 opt

<400>  7428
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauguccc       60

aaaaaguaau auaccucguc augauacucc ucauagcccc cgcauacagc auacgcugca      120

ucggggucuc caacagagac uucgucgaag gaaugagcgg aggcacaugg guggacguag      180

uccuggaaca cggaggaugc guaaccguaa uggcccaaga caaaccaacc gucgacauag      240

agcuaguuac aaccaccguc uccaacaugg ccgaaguccg cuccuacugc uacgaagcca      300

gcauaagcga cauggccagc gacagcagau gcccgaccca aggcgaagcc uaccuagaca      360

aacaaaguga cacccaauac gucugcaaac gaacacucgu agaucgcggc uggggcaacg      420

gcugcggucu guucggaaaa ggaagccuag uaaccugcgc caaauucaca ugcagcaaga      480

aaaugaccgg aaaaucaauc cagcccgaaa accucgaaua ccgcauaaug cuauccguac      540

acgguagcca acacuccggc augaucguca acgacaucgg acacgagaca gacgaaaacc      600

gcgccaaagu agaaguaacc ccaaacuccc ccagggccga agcaacacuc ggaggcuucg      660

gaucccuagg acuagacugc gaacccagaa ccggccucga uuucagcgac cuauacuacc      720

ucacgaugaa caacaaacac uggcucguac acaaagaaug guuccacgac auaccccuac      780

ccuggcacgc cggcgcagac accggcacac cccacuggaa caacaaagaa gcacucguag      840

aguucaaaga cgcacacgca aaacgccaaa ccguaguagu ccuaggcagc caagaaggcg      900

ccguacacac cgcccuggcc ggagcgcuag aagcagaaau ggacggagcc aaaggcagac      960

uauucagcgg ccaccuaaaa ugccgacuaa aaauggacaa acucagacuc aaaggaguaa     1020

gcuacagccu augcaccgcc guaugcaccg ccgccaaagu ccccgcagaa acacuacacg     1080

gcacagucac aguagaaguc caauacgcag gcacggacgg ccccugcaaa gugccagccc     1140

aaauggccgu agacaugcaa acucugacac cagucggcag acucauaacc gccaacccag     1200

uaauaacaga aagcacggaa aacucaaaaa ugaugcuuga acucgacccg cccuucggag     1260

acucauacau agucauagga guaggcgaca agaaaaucac acaccacugg caccggagcg     1320

gcuccacuau aggcaaagca uucgaagcaa ccguaagagg agccaaacga auggccguac     1380

uaggggacac agcaugggac uucggcuccu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7429
<211>  1723
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 2 opt

<400>  7429
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucgc       60

aaaaagucau auaccugguc augauccugc uuaucgcacc cgcuuacucc auccgcugca      120

ucggagucuc gaacagggac uucgucgaag gcaugagcgg aggcacaugg guagacguag      180

uccuagaaca cggcggcugc gugaccguca uggcacaaga caaaccgaca guagacauag      240

aacucgucac aaccacaguc ucgaacaugg cagaaguuag aaguuacugc uacgaagcca      300

gcauaagcga cauggcaagc gacagcagau gcccaaccca aggggaagca uaccuggaca      360

aacaaagcga cacccaauac gucugcaaac gcacgcuagu agacagggga uggggcaacg      420

gaugcggacu guucgggaaa ggaagccuag ucacaugcgc gaaguucaca ugcagcaaga      480

aaaugacagg aaaauccaua caacccgaaa accuagaaua ccgaauaaug cugagcgugc      540

acggaagcca acacagcggc augauaguaa acgacgaaaa ccgcgcgaaa guagaaguaa      600

ccccaaacag cccaagggcc gaggcaacgc uaggaggauu cggaagccua gggcucgacu      660

gcgagcccag aaccggacuc gacuucucgg accuauacua ccuaacgaug aacaacaaac      720

acuggcuagu acacaaagaa ugguuccacg acauaccgcu ccccuggcac ucgggagcag      780

acaccgaaac gccacacugg aacaacaaag aagcgcuagu agaguucaaa gacgcccacg      840

ccaagcgaca gacaguaguc guacucggga gccaggaagg agccguacac acagcccucg      900

ccggagcccu cgaagcagag auggacggag ccaaaggccg acuaucgucc ggccaccuaa      960

aaugccgccu gaaaauggac aaacucaggc uaaaaggcgu gagcuacagc cugugcaccg     1020

ccgcauucac auucacaaaa gugcccgcag aaacacucca uggaacaguc acagucgaag     1080

uccaauacgc aggaagggac gggcccugca aaguacccgc gcagauggca guagacaugc     1140

aaacgcuaac ccccgucggc agacucauaa cagcgaaccc ggucauaacc gaaagcacag     1200

aaaacagcaa aaugaugcua gaauuagauc ccccauucgg ggacucauac aucgucauag     1260

gcguaggcga caagaaaaua acacaccacu ggcacagauc aggcuccaua auaggcaaag     1320

ccuucgaagc aacagugcga ggagccaaac gcauggcagu acuaggagac accgccuggg     1380

acuucggcuc augaggacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1440

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1500

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1560

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     1680

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       1723


<210>  7430
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 2 opt

<400>  7430
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugucac       60

aaaagguaau auaccuaguc augaucguac ucauaguccc ggcauacagc auaagcugua      120

ucggaguauc aaaccgagac cugguagagg gcaugagcgg gggcacaugg guggacgugg      180

uacuggaaca uggcggaugc guaaccgaaa uggcgcaaga caaaccgacc guggacauag      240

agcuaguaac aaugaccguc ucgaacaugg cagaagucag aagcuacugc uacgaagcaa      300

gccugagcga cauggcaagc gcaaguaggu gcccgaccca aggagaacca ucacuagaca      360

aacaaagcga cacccagagc guaugcaaga gaacgcuagg ggacagaggc uggggaaacg      420

gcugcggaau cuucggaaaa ggaucccuag ucacgugcuc caaauuuaca ugcugcaaga      480

aaaugccagg gaaaagcauc cagccagaga accucgaaua uagaauaaug cuacccgugc      540

acggcagcca gcacucagga augaucguga acgacaucgg acacgagacc gacgaaaacc      600

gcgcaaaagu ggaaguaacc ccaaacucac ccagagcaga agccacgcuc ggaggauucg      660

gaagccucgg gcucgacugc gaaccccgaa ccgggcucga cuucucagac uuauacuacc      720

uaacaaugaa caacaagcac uggcucguac acaaagagug guuccacgac auaccccugc      780

cauggcacgc gggagcagac acaggcacgc cgcauuggaa caacaaggag gcacucguag      840

aguucaaaga cgcgcacgca aagcgacaaa cagucguagu gcucggaagc caagaagggg      900

ccguccacac agccuuagcc ggggcccucg aagcagaaau ggacggggca aagggccggc      960

ucuuuagcgg ccaccuaaaa ugccgccuaa aaauggacaa gcuaagacua aagggcguaa     1020

gcuacagccu gugcacagcc gcauucacau ucacaaaagu acccgcagaa acgcuccacg     1080

gcacagucac agucgagguc cagagcgcgg gaacggacgg acccugcaaa guaccagcgc     1140

aaauggccgu agacaugcaa acacucaccc ccgugggaag gcuaauaaca gcaaacccgg     1200

uaauaaccga aagcacagaa aacagcaaga ugaugcucga guuagaccca ccauucgggg     1260

acucauacau aguuauaggc guaggcgaca agaaaaucac gcaccauugg cacagauccg     1320

gaaguaccau cggcaaagcc uucgaagcaa ccgugcgcgg ggccaagaga auggcagugc     1380

ucggagauac cgcaugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7431
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 2 opt

<400>  7431
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugcgac       60

aaaaagucau uuaccuagua augauacucc uaauagcccc agccuacagc auuagaugca      120

ucgggguauc aaacagggac uucguagaag gaaugagcgg cggaaccugg guagacgucg      180

uccucgaaca cggcggcugc guaaccguaa uggcacaaga caaaccgacc guagacauag      240

aacucgucac caccacaguc ucaaacaugg cagaaguccg gagcuacugc uacgaggcuu      300

cuauaagcga cauggcaagc gacagcagau gcccaaccca aggcgaagca uaccuagaca      360

aacaaagcga cacccaguac guaugcaaaa gaacccucgu agaccgagga uggggcaacg      420

gcugcggccu guucggaaaa ggaagccugg uaacaugcgc aaaauuugcc ugcagcaaaa      480

agaugacagg gaaauccauc caacccgaaa accuagaaua cagaauaaug cucagcguac      540

auggaagcca acauagcggc augaucguca acgacacagg acacgagaca gacgaaaacc      600

gcgcaaaagu agaaaucacc ccaaacucac ccagggccga ggcaacacuc ggcggauucg      660

gcagccucgg ccucgacugc gaacccagaa ccggccucga cuucucagac cuguacuauc      720

uaaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac aucccacugc      780

ccuggcacgc cggcgccgac acaggaacac cacacuggaa caacaaagaa gcccuagucg      840

aguucaaaga cgcacacgca aaacgacaaa cagucgucgu ccucggcagc caagaaggag      900

caguccacac ggcccuggca ggagcccucg aagcagaaau ggacggagcc aaaggcaggc      960

ucucaucagg acaccuaaaa ugccgccuua aaauggacaa acuccgacug aaaggaguau     1020

ccuacagccu cugcacagcc gcauucaccu ucacaaaaau accagcggaa acacugcacg     1080

gcacagucac cgucgaaguc caguacgcag gcaccgacgg ccccugcaaa guaccagcac     1140

aaauggcggu ggacaugcaa acccuaaccc caguaggccg acuaauaacc gcgaacccag     1200

ucauaaccga gagcaccgaa aacaguaaaa ugaugcucga gcucgaccca ccauucggag     1260

acagcuacau aguaauaggc gucggugaaa agaaaaucac acaccacugg caccgcucgg     1320

gcucaaccau cggcaaagcc uucgaagcaa cuguacgcgg agccaaacgc auggcaguac     1380

uaggagacac agcaugggac uucggcucau gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7432
<211>  1741
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 2 opt

<400>  7432
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugagcc       60

aaagagucau cuaccuggua augauccuac uaauagcccc agccuacagc auccgaugca      120

ucggggucuc gaacagagac uucgucgaag gcaugagcgg aggcacaugg guagacguag      180

ugcuggaaca cggcggaugc guaaccguaa uggcacaaga caaaccaacc guagacauag      240

agcuagucac aacaacaguc ucaaacaugg ccgaagucag gaguuacugc uacgaagcaa      300

gcauaagcga cauggccagc gacagccgau gcccaaccca aggcgaagca uaccuagaca      360

aacaaagcga cacccaauac gugugcaaac gcacgcuagu cgacagagga uggggcaacg      420

ggugcggccu cuucgggaaa ggaagccuag ucacgugcgc aaaauucaca ugcagcaaga      480

agaugaccgg aaaaucaauc caacccgaaa accuagaaua ccgcauaaug cugagcgucc      540

acggaagcca acacucaggc augaucguca acgacaucgg acacgagacc gacgaaaacc      600

gagcgaaagu ggaaguaacc ccaaacucac caagggccga ggccacccuc ggaggcuucg      660

gaucgcucgg gcuagacugc gaacccagaa ccggacucga cuucucagac cucuacuacc      720

ucacgaugaa caacaaacau uggcucgugc acaaagaaug guuccacgau aucccacugc      780

ccuggcacgc aggcgcggac acaggaacac cccacuggaa caauaaggaa gcacuuguag      840

aguucaaaga cgcccacgcc aaacgccaga ccguagucgu acuaggaagc caagaaggag      900

ccgugcacac cgcccuggca ggagcacugg aagcagaaau ggacggagcc aaaggccgac      960

ucuucagcgg acacuugaaa ugccgcauaa aaauggacaa acucaggcuc aaaggcguga     1020

gcuacagccu augcacugcc gcauucacau ucuccaaggu accagcagaa acacuccacg     1080

gcaccgucac gguagaaguc caauacgcag gccgagacgg gccaugcaaa gugcccgcac     1140

aaauggccgu agacaugcaa acgcucacac ccguaggcag gcucauaaca gcgaacccaa     1200

ucauaacaga aaguacagaa aacagcagaa ugaugcuaga acucgaccca ccguucgggg     1260

acuccuacau agucaucggc gugggcgaca agaaaauaac acaccacugg cauagaucgg     1320

gcucaaccau aggcaaagcc uucgaagcga cagugcgagg agcaaaaaga auggccguac     1380

uaggagacac agcaugggau uucggcucgu gaggacuagu gcaucacauu uaaaagcauc     1440

ucagccuacc augagaauaa gagaaagaaa augaagauca auagcuuauu caucucuuuu     1500

ucuuuuucgu ugguguaaag ccaacacccu gucuaaaaaa cauaaauuuc uuuaaucauu     1560

uugccucuuu ucucugugcu ucaauuaaua aaaaauggaa agaaccuaga ucuaaaaaaa     1620

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     1680

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     1740

u                                                                     1741


<210>  7433
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4803/R4833 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 3 wild type

<400>  7433
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7434
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4815/R4837 ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA 
       Design 3 wild type

<400>  7434
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacgagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaaucuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7435
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4819/R4841 ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 
       3 wild type

<400>  7435
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1080

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1140

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1200

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1260

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1320

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1380

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1440

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug     1500

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1560

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1620

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1680

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1740

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1800

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1860

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1920

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1980

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     2040

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2100

gcuguuucug cugacguggg gugcucagug gacuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7436
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4867 ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 3 
       wild type

<400>  7436
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7437
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4879/R5080 ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA 
       Design 3 wild type

<400>  7437
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7438
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 3 wild 
       type

<400>  7438
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7439
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 3 wild type

<400>  7439
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7440
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4869/R5114 ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA 
       Design 3 wild type

<400>  7440
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7441
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 3 wild
       type

<400>  7441
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7442
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 3 wild 
       type

<400>  7442
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7443
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 3 wild type

<400>  7443
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      540

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      600

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      660

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      720

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag      780

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc      840

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca      900

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug      960

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1020

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1080

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1140

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1200

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1260

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1320

auggcagucc ugggggauac agccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  7444
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 3 wild 
       type

<400>  7444
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7445
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       3 wild type

<400>  7445
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuc cggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7446
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 3
       wild type

<400>  7446
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuc cggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7447
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 3 
       wild type

<400>  7447
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuc cggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7448
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F398S) mRNA Design 3 
       wild type

<400>  7448
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuc cggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7449
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       3 wild type

<400>  7449
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguucagg acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7450
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 3
       wild type

<400>  7450
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguucagg acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7451
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 3 
       wild type

<400>  7451
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguucagg acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7452
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 wild 
       type

<400>  7452
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7453
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 wild 
       type

<400>  7453
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7454
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 3 wild type

<400>  7454
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gaguggcagc acgcugggca aggccuuuuc aacaacuuug     1800

aagggagcuc aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga     1860

ggggucuuca acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca     1920

cucuuugggg gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug     1980

ggcgucaacg cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc     2040

guguucuuag cgaccaaugu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7455
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 wild 
       type

<400>  7455
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7456
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 wild 
       type

<400>  7456
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7457
<211>  2362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7457
gggagaaagc uuaccaugau aaucaaugcu aggaaggaga agaagagacg gggcgcagau       60

accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga      120

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      180

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      240

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      300

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      360

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      420

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      480

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      540

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      600

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      660

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      720

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      780

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      840

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      900

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      960

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac     1020

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1080

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1140

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1200

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1260

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1320

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1380

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1440

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1500

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1560

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1620

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1680

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1740

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1800

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1860

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1920

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1980

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     2040

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2100

uuauccacag ccgucucugc ugaugugggg ugcucggugg acuucucaaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2340

ucuuuucaga gccaccagaa uu                                              2362


<210>  7458
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7458
gggagaaagc uuaccaugau caaugcuagg aaggagaaga agagacgggg cgcagauacc       60

agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu      120

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      180

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      240

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      300

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      360

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      420

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      480

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      540

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      600

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      660

acuuggguug auauugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      720

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      780

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      840

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      900

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gucuggugac augcgcuaag      960

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg     1020

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1080

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1140

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1200

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1260

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1320

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1380

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1440

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1500

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1560

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1620

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1680

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1740

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1800

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1860

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1920

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1980

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     2040

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2100

uccacagccg ucucugcuga uguggggugc ucgguggacu ucucaaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  7459
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7459
gggagaaagc uuaccaugaa ugcuaggaag gagaagaaga gacggggcgc agauaccagu       60

gucggaauug uuggccuccu gcugaccaca gcuauggcag cggaggucac uagacguggg      120

agugcauacu auauguacuu ggacagaaac gaugcugggg aggccauauc uuuuccaacc      180

acauugggga ugaauaagug uuauauacag aucauggauc uuggacacau gugugaugcc      240

accaugagcu augaaugccc uaugcuggau gagggggugg aaccagauga cgucgauugu      300

uggugcaaca cgacgucaac uuggguugug uacggaaccu gccaucacaa aaaaggugaa      360

gcacggagau cuagaagagc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg      420

cggucgcaaa ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau      480

uggauauuca ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga      540

agcucaacga gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac      600

agcaucaggu gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu      660

uggguugaua uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg      720

acugucgaca uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac      780

ugcuaugagg caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa      840

gccuaccuug acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga      900

ggcuggggaa auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu      960

gcaugcucca agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua     1020

augcugucag uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa     1080

acugaugaga auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc     1140

cugggggguu uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca     1200

gauuuguauu acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac     1260

gacauuccau uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa     1320

gaagcacugg uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg     1380

agucaagaag gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu     1440

gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga     1500

uugaagggcg ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu     1560

gaaacacugc acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc     1620

aagguuccag cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua     1680

accgcuaacc ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau     1740

ccaccauuug gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac     1800

uggcacagga guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag     1860

agaauggcag ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac     1920

ucauugggca agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga     1980

auguccuggu ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca     2040

aagaauggau cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc     2100

acagccgucu cugcugaugu ggggugcucg guggacuucu caaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  7460
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7460
gggagaaagc uuaccauggc uaggaaggag aagaagagac ggggcgcaga uaccaguguc       60

ggaauuguug gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu      120

gcauacuaua uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca      180

uuggggauga auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc      240

augagcuaug aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg      300

ugcaacacga cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca      360

cggagaucua gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      420

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      480

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      540

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      600

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      660

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      720

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      780

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      840

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      900

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      960

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug     1020

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu     1080

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug     1140

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau     1200

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac     1260

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1320

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1380

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1440

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1500

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1560

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1620

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1680

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1740

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1800

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1860

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1920

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1980

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     2040

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     2100

gccgucucug cugauguggg gugcucggug gacuucucaa agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7461
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7461
gggagaaagc uuaccaugag gaaggagaag aagagacggg gcgcagauac cagugucgga       60

auuguuggcc uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca      120

uacuauaugu acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug      180

gggaugaaua aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug      240

agcuaugaau gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc      300

aacacgacgu caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg      360

agaucuagaa gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      420

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      480

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      540

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      600

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      660

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      720

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      780

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      840

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      900

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      960

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug     1020

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau     1080

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg     1140

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug     1200

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu     1260

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1320

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1380

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1440

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1500

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1560

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1620

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1680

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1740

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1800

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1860

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1920

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1980

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     2040

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     2100

gucucugcug auguggggug cucgguggac uucucaaagu gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2220

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  7462
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7462
gggagaaagc uuaccaugaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7463
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7463
gggagaaagc uuaccaugga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugaugugg ggugcucggu ggacuucuca aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  7464
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7464
gggagaaagc uuaccaugaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gauguggggu gcucggugga cuucucaaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  7465
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7465
gggagaaagc uuaccaugaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100

guggggugcu cgguggacuu cucaaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7466
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7466
gggagaaagc uuaccaugag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugaugug     2100

gggugcucgg uggacuucuc aaagugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  7467
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7467
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcucggugg acuucucaaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7468
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7468
gggagaaagc uuaccauggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga uguggggugc     2100

ucgguggacu ucucaaagug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7469
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7469
gggagaaagc uuaccauggc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcucg     2100

guggacuucu caaagugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7470
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7470
gggagaaagc uuaccaugga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcucggug     2100

gacuucucaa agugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  7471
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7471
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cucgguggac     2100

uucucaaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7472
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7472
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggccg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cucgguggac     2100

uucucaaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7473
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7473
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcggc cgucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcucggugg acuucucaaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7474
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7474
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg guggagugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  7475
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7475
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg gugccgugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  7476
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7476
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7477
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7477
gggagaaagc uuaccaugau aaucaaugcu aggaaggaga agaagagacg gggcgcagau       60

accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga      120

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      180

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      240

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      300

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      360

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      420

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      480

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      540

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      600

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      660

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      720

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      780

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      840

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      900

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      960

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac     1020

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1080

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1140

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1200

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1260

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1320

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1380

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1440

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1500

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1560

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1620

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1680

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1740

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1800

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1860

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1920

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1980

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     2040

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2100

uuauccacag ccgucucugc ugaugugggg ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  7478
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7478
gggagaaagc uuaccaugau caaugcuagg aaggagaaga agagacgggg cgcagauacc       60

agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu      120

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      180

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      240

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      300

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      360

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      420

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      480

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      540

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      600

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      660

acuuggguug auauugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      720

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      780

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      840

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      900

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gucuggugac augcgcuaag      960

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg     1020

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1080

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1140

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1200

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1260

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1320

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1380

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1440

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1500

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1560

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1620

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1680

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1740

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1800

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1860

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1920

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1980

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     2040

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2100

uccacagccg ucucugcuga uguggggugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  7479
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7479
gggagaaagc uuaccaugaa ugcuaggaag gagaagaaga gacggggcgc agauaccagu       60

gucggaauug uuggccuccu gcugaccaca gcuauggcag cggaggucac uagacguggg      120

agugcauacu auauguacuu ggacagaaac gaugcugggg aggccauauc uuuuccaacc      180

acauugggga ugaauaagug uuauauacag aucauggauc uuggacacau gugugaugcc      240

accaugagcu augaaugccc uaugcuggau gagggggugg aaccagauga cgucgauugu      300

uggugcaaca cgacgucaac uuggguugug uacggaaccu gccaucacaa aaaaggugaa      360

gcacggagau cuagaagagc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg      420

cggucgcaaa ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau      480

uggauauuca ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga      540

agcucaacga gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac      600

agcaucaggu gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu      660

uggguugaua uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg      720

acugucgaca uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac      780

ugcuaugagg caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa      840

gccuaccuug acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga      900

ggcuggggaa auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu      960

gcaugcucca agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua     1020

augcugucag uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa     1080

acugaugaga auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc     1140

cugggggguu uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca     1200

gauuuguauu acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac     1260

gacauuccau uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa     1320

gaagcacugg uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg     1380

agucaagaag gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu     1440

gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga     1500

uugaagggcg ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu     1560

gaaacacugc acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc     1620

aagguuccag cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua     1680

accgcuaacc ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau     1740

ccaccauuug gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac     1800

uggcacagga guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag     1860

agaauggcag ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac     1920

ucauugggca agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga     1980

auguccuggu ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca     2040

aagaauggau cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc     2100

acagccgucu cugcugaugu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7480
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7480
gggagaaagc uuaccauggc uaggaaggag aagaagagac ggggcgcaga uaccaguguc       60

ggaauuguug gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu      120

gcauacuaua uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca      180

uuggggauga auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc      240

augagcuaug aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg      300

ugcaacacga cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca      360

cggagaucua gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      420

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      480

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      540

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      600

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      660

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      720

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      780

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      840

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      900

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      960

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug     1020

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu     1080

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug     1140

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau     1200

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac     1260

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1320

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1380

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1440

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1500

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1560

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1620

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1680

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1740

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1800

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1860

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1920

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1980

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     2040

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     2100

gccgucucug cugauguggg gugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  7481
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7481
gggagaaagc uuaccaugag gaaggagaag aagagacggg gcgcagauac cagugucgga       60

auuguuggcc uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca      120

uacuauaugu acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug      180

gggaugaaua aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug      240

agcuaugaau gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc      300

aacacgacgu caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg      360

agaucuagaa gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      420

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      480

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      540

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      600

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      660

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      720

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      780

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      840

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      900

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      960

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug     1020

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau     1080

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg     1140

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug     1200

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu     1260

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1320

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1380

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1440

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1500

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1560

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1620

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1680

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1740

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1800

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1860

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1920

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1980

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     2040

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     2100

gucucugcug auguggggug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7482
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7482
gggagaaagc uuaccaugaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugaug uggggugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7483
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7483
gggagaaagc uuaccaugga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugaugugg ggugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7484
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7484
gggagaaagc uuaccaugaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gauguggggu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  7485
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7485
gggagaaagc uuaccaugaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100

guggggugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7486
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7486
gggagaaagc uuaccaugag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugaugug     2100

gggugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  7487
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7487
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  7488
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7488
gggagaaagc uuaccauggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga uguggggugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  7489
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7489
gggagaaagc uuaccauggc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugaugu ggggugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  7490
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7490
gggagaaagc uuaccaugga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauguggg gugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  7491
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7491
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7492
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7492
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggccg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auguggggug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7493
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7493
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcggc cgucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugaugugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  7494
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7494
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg guggagugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7495
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7495
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg gugccgugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7496
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7496
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugauuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7497
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7497
gggagaaagc uuaccaugau aaucaaugcu aggaaggaga agaagagacg gggcgcagau       60

accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga      120

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      180

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      240

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      300

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      360

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      420

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      480

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      540

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      600

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      660

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      720

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      780

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      840

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      900

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      960

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac     1020

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1080

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1140

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1200

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1260

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1320

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1380

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1440

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1500

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1560

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1620

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1680

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1740

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1800

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1860

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1920

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1980

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     2040

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2100

uuauccacag ccgucucugc ugauugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  7498
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7498
gggagaaagc uuaccaugau caaugcuagg aaggagaaga agagacgggg cgcagauacc       60

agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu      120

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      180

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      240

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      300

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      360

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      420

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      480

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      540

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      600

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      660

acuuggguug auauugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      720

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      780

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      840

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      900

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gucuggugac augcgcuaag      960

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg     1020

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1080

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1140

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1200

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1260

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1320

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1380

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1440

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1500

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1560

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1620

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1680

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1740

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1800

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1860

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1920

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1980

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     2040

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2100

uccacagccg ucucugcuga uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7499
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7499
gggagaaagc uuaccaugaa ugcuaggaag gagaagaaga gacggggcgc agauaccagu       60

gucggaauug uuggccuccu gcugaccaca gcuauggcag cggaggucac uagacguggg      120

agugcauacu auauguacuu ggacagaaac gaugcugggg aggccauauc uuuuccaacc      180

acauugggga ugaauaagug uuauauacag aucauggauc uuggacacau gugugaugcc      240

accaugagcu augaaugccc uaugcuggau gagggggugg aaccagauga cgucgauugu      300

uggugcaaca cgacgucaac uuggguugug uacggaaccu gccaucacaa aaaaggugaa      360

gcacggagau cuagaagagc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg      420

cggucgcaaa ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau      480

uggauauuca ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga      540

agcucaacga gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac      600

agcaucaggu gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu      660

uggguugaua uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg      720

acugucgaca uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac      780

ugcuaugagg caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa      840

gccuaccuug acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga      900

ggcuggggaa auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu      960

gcaugcucca agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua     1020

augcugucag uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa     1080

acugaugaga auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc     1140

cugggggguu uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca     1200

gauuuguauu acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac     1260

gacauuccau uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa     1320

gaagcacugg uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg     1380

agucaagaag gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu     1440

gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga     1500

uugaagggcg ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu     1560

gaaacacugc acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc     1620

aagguuccag cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua     1680

accgcuaacc ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau     1740

ccaccauuug gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac     1800

uggcacagga guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag     1860

agaauggcag ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac     1920

ucauugggca agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga     1980

auguccuggu ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca     2040

aagaauggau cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc     2100

acagccgucu cugcugauug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7500
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7500
gggagaaagc uuaccauggc uaggaaggag aagaagagac ggggcgcaga uaccaguguc       60

ggaauuguug gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu      120

gcauacuaua uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca      180

uuggggauga auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc      240

augagcuaug aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg      300

ugcaacacga cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca      360

cggagaucua gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      420

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      480

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      540

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      600

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      660

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      720

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      780

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      840

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      900

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      960

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug     1020

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu     1080

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug     1140

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau     1200

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac     1260

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1320

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1380

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1440

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1500

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1560

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1620

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1680

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1740

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1800

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1860

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1920

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1980

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     2040

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     2100

gccgucucug cugauugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7501
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7501
gggagaaagc uuaccaugag gaaggagaag aagagacggg gcgcagauac cagugucgga       60

auuguuggcc uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca      120

uacuauaugu acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug      180

gggaugaaua aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug      240

agcuaugaau gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc      300

aacacgacgu caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg      360

agaucuagaa gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      420

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      480

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      540

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      600

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      660

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      720

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      780

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      840

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      900

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      960

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug     1020

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau     1080

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg     1140

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug     1200

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu     1260

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1320

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1380

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1440

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1500

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1560

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1620

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1680

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1740

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1800

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1860

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1920

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1980

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     2040

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     2100

gucucugcug auugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  7502
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7502
gggagaaagc uuaccaugaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcugauu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7503
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7503
gggagaaagc uuaccaugga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcugauugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  7504
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7504
gggagaaagc uuaccaugaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

gauugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  7505
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7505
gggagaaagc uuaccaugaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcugau     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  7506
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7506
gggagaaagc uuaccaugag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcugauuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  7507
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7507
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugauugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  7508
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7508
gggagaaagc uuaccauggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuga uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7509
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7509
gggagaaagc uuaccauggc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcugauug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  7510
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7510
gggagaaagc uuaccaugga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cugauugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  7511
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7511
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  7512
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7512
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggccg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcug auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  7513
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7513
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcggc cgucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc ugauugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  7514
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7514
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg guggagugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7515
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7515
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg gugccgugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7516
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7516
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcuugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  7517
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7517
gggagaaagc uuaccaugau caaugcuagg aaggagaaga agagacgggg cgcagauacc       60

agugucggaa uuguuggccu ccugcugacc acagcuaugg cagcggaggu cacuagacgu      120

gggagugcau acuauaugua cuuggacaga aacgaugcug gggaggccau aucuuuucca      180

accacauugg ggaugaauaa guguuauaua cagaucaugg aucuuggaca caugugugau      240

gccaccauga gcuaugaaug cccuaugcug gaugaggggg uggaaccaga ugacgucgau      300

uguuggugca acacgacguc aacuuggguu guguacggaa ccugccauca caaaaaaggu      360

gaagcacgga gaucuagaag agcugugacg cuccccuccc auuccacuag gaagcugcaa      420

acgcggucgc aaaccugguu ggaaucaaga gaauacacaa agcacuugau uagagucgaa      480

aauuggauau ucaggaaccc uggcuucgcg uuagcagcag cugccaucgc uuggcuuuug      540

ggaagcucaa cgagccaaaa agucauauac uuggucauga uacugcugau ugccccggca      600

uacagcauca ggugcauagg agucagcaau agggacuuug uggaagguau gucagguggg      660

acuuggguug auauugucuu ggaacaugga gguuguguca ccguaauggc acaggacaaa      720

ccgacugucg acauagagcu gguuacaaca acagucagca acauggcgga gguaagaucc      780

uacugcuaug aggcaucaau aucagacaug gcuucggaca gccgcugccc aacacaaggu      840

gaagccuacc uugacaagca aucagacacu caauaugucu gcaaaagaac guuaguggac      900

agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga gucuggugac augcgcuaag      960

uuugcaugcu ccaagaaaau gaccgggaag agcauccagc cagagaaucu ggaguaccgg     1020

auaaugcugu caguucaugg cucccagcac agugggauga ucguuaauga cacaggacau     1080

gaaacugaug agaauagagc gaagguugag auaacgccca auucaccaag agccgaagcc     1140

acccuggggg guuuuggaag ccuaggacuu gauugugaac cgaggacagg ccuugacuuu     1200

ucagauuugu auuacuugac uaugaauaac aagcacuggu ugguucacaa ggagugguuc     1260

cacgacauuc cauuaccuug gcacgcuggg gcagacaccg gaacuccaca cuggaacaac     1320

aaagaagcac ugguagaguu caaggacgca caugccaaaa ggcaaacugu cgugguucua     1380

gggagucaag aaggagcagu ucacacggcc cuugcuggag cucuggaggc ugagauggau     1440

ggugcaaagg gaaggcuguc cucuggccac uugaaauguc gccugaaaau ggauaaacuu     1500

agauugaagg gcgugucaua cuccuugugu accgcagcgu ucacauucac caagaucccg     1560

gcugaaacac ugcacgggac agucacagug gagguacagu acgcagggac agauggaccu     1620

ugcaagguuc cagcucagau ggcgguggac augcaaacuc ugaccccagu ugggagguug     1680

auaaccgcua accccguaau cacugaaagc acugagaacu cuaagaugau gcuggaacuu     1740

gauccaccau uuggggacuc uuacauuguc auaggagucg gggagaagaa gaucacccac     1800

cacuggcaca ggaguggcag caccauugga aaagcauuug aagccacugu gagaggugcc     1860

aagagaaugg cagucuuggg agacacagcc ugggacuuug gaucaguugg aggcgcucuc     1920

aacucauugg gcaagggcau ccaucaaauu uuuggagcag cuuucaaauc auuguuugga     1980

ggaauguccu gguucucaca aauucucauu ggaacguugc ugaugugguu gggucugaac     2040

acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag ggggaguguu gaucuucuua     2100

uccacagccg ucucugcuug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7518
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7518
gggagaaagc uuaccaugau aaucaaugcu aggaaggaga agaagagacg gggcgcagau       60

accagugucg gaauuguugg ccuccugcug accacagcua uggcagcgga ggucacuaga      120

cgugggagug cauacuauau guacuuggac agaaacgaug cuggggaggc cauaucuuuu      180

ccaaccacau uggggaugaa uaaguguuau auacagauca uggaucuugg acacaugugu      240

gaugccacca ugagcuauga augcccuaug cuggaugagg ggguggaacc agaugacguc      300

gauuguuggu gcaacacgac gucaacuugg guuguguacg gaaccugcca ucacaaaaaa      360

ggugaagcac ggagaucuag aagagcugug acgcuccccu cccauuccac uaggaagcug      420

caaacgcggu cgcaaaccug guuggaauca agagaauaca caaagcacuu gauuagaguc      480

gaaaauugga uauucaggaa cccuggcuuc gcguuagcag cagcugccau cgcuuggcuu      540

uugggaagcu caacgagcca aaaagucaua uacuugguca ugauacugcu gauugccccg      600

gcauacagca ucaggugcau aggagucagc aauagggacu uuguggaagg uaugucaggu      660

gggacuuggg uugauauugu cuuggaacau ggagguugug ucaccguaau ggcacaggac      720

aaaccgacug ucgacauaga gcugguuaca acaacaguca gcaacauggc ggagguaaga      780

uccuacugcu augaggcauc aauaucagac auggcuucgg acagccgcug cccaacacaa      840

ggugaagccu accuugacaa gcaaucagac acucaauaug ucugcaaaag aacguuagug      900

gacagaggcu ggggaaaugg auguggacuu uuuggcaaag ggagucuggu gacaugcgcu      960

aaguuugcau gcuccaagaa aaugaccggg aagagcaucc agccagagaa ucuggaguac     1020

cggauaaugc ugucaguuca uggcucccag cacaguggga ugaucguuaa ugacacagga     1080

caugaaacug augagaauag agcgaagguu gagauaacgc ccaauucacc aagagccgaa     1140

gccacccugg gggguuuugg aagccuagga cuugauugug aaccgaggac aggccuugac     1200

uuuucagauu uguauuacuu gacuaugaau aacaagcacu gguugguuca caaggagugg     1260

uuccacgaca uuccauuacc uuggcacgcu ggggcagaca ccggaacucc acacuggaac     1320

aacaaagaag cacugguaga guucaaggac gcacaugcca aaaggcaaac ugucgugguu     1380

cuagggaguc aagaaggagc aguucacacg gcccuugcug gagcucugga ggcugagaug     1440

gauggugcaa agggaaggcu guccucuggc cacuugaaau gucgccugaa aauggauaaa     1500

cuuagauuga agggcguguc auacuccuug uguaccgcag cguucacauu caccaagauc     1560

ccggcugaaa cacugcacgg gacagucaca guggagguac aguacgcagg gacagaugga     1620

ccuugcaagg uuccagcuca gauggcggug gacaugcaaa cucugacccc aguugggagg     1680

uugauaaccg cuaaccccgu aaucacugaa agcacugaga acucuaagau gaugcuggaa     1740

cuugauccac cauuugggga cucuuacauu gucauaggag ucggggagaa gaagaucacc     1800

caccacuggc acaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggu     1860

gccaagagaa uggcagucuu gggagacaca gccugggacu uuggaucagu uggaggcgcu     1920

cucaacucau ugggcaaggg cauccaucaa auuuuuggag cagcuuucaa aucauuguuu     1980

ggaggaaugu ccugguucuc acaaauucuc auuggaacgu ugcugaugug guugggucug     2040

aacacaaaga auggaucuau uucccuuaug ugcuuggccu uagggggagu guugaucuuc     2100

uuauccacag ccgucucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7519
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7519
gggagaaagc uuaccaugaa ugcuaggaag gagaagaaga gacggggcgc agauaccagu       60

gucggaauug uuggccuccu gcugaccaca gcuauggcag cggaggucac uagacguggg      120

agugcauacu auauguacuu ggacagaaac gaugcugggg aggccauauc uuuuccaacc      180

acauugggga ugaauaagug uuauauacag aucauggauc uuggacacau gugugaugcc      240

accaugagcu augaaugccc uaugcuggau gagggggugg aaccagauga cgucgauugu      300

uggugcaaca cgacgucaac uuggguugug uacggaaccu gccaucacaa aaaaggugaa      360

gcacggagau cuagaagagc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg      420

cggucgcaaa ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau      480

uggauauuca ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga      540

agcucaacga gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac      600

agcaucaggu gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu      660

uggguugaua uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg      720

acugucgaca uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac      780

ugcuaugagg caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa      840

gccuaccuug acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga      900

ggcuggggaa auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu      960

gcaugcucca agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua     1020

augcugucag uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa     1080

acugaugaga auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc     1140

cugggggguu uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca     1200

gauuuguauu acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac     1260

gacauuccau uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa     1320

gaagcacugg uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg     1380

agucaagaag gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu     1440

gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga     1500

uugaagggcg ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu     1560

gaaacacugc acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc     1620

aagguuccag cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua     1680

accgcuaacc ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau     1740

ccaccauuug gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac     1800

uggcacagga guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag     1860

agaauggcag ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac     1920

ucauugggca agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga     1980

auguccuggu ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca     2040

aagaauggau cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc     2100

acagccgucu cugcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7520
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7520
gggagaaagc uuaccauggc uaggaaggag aagaagagac ggggcgcaga uaccaguguc       60

ggaauuguug gccuccugcu gaccacagcu auggcagcgg aggucacuag acgugggagu      120

gcauacuaua uguacuugga cagaaacgau gcuggggagg ccauaucuuu uccaaccaca      180

uuggggauga auaaguguua uauacagauc auggaucuug gacacaugug ugaugccacc      240

augagcuaug aaugcccuau gcuggaugag gggguggaac cagaugacgu cgauuguugg      300

ugcaacacga cgucaacuug gguuguguac ggaaccugcc aucacaaaaa aggugaagca      360

cggagaucua gaagagcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      420

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      480

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      540

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      600

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      660

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      720

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      780

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      840

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      900

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      960

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug     1020

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu     1080

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug     1140

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau     1200

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac     1260

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1320

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1380

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1440

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1500

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1560

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1620

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1680

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1740

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1800

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1860

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1920

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1980

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     2040

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     2100

gccgucucug cuugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  7521
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7521
gggagaaagc uuaccaugag gaaggagaag aagagacggg gcgcagauac cagugucgga       60

auuguuggcc uccugcugac cacagcuaug gcagcggagg ucacuagacg ugggagugca      120

uacuauaugu acuuggacag aaacgaugcu ggggaggcca uaucuuuucc aaccacauug      180

gggaugaaua aguguuauau acagaucaug gaucuuggac acauguguga ugccaccaug      240

agcuaugaau gcccuaugcu ggaugagggg guggaaccag augacgucga uuguuggugc      300

aacacgacgu caacuugggu uguguacgga accugccauc acaaaaaagg ugaagcacgg      360

agaucuagaa gagcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      420

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      480

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      540

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      600

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      660

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      720

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      780

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      840

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      900

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      960

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug     1020

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau     1080

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg     1140

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug     1200

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu     1260

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1320

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1380

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1440

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1500

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1560

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1620

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1680

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1740

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1800

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1860

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1920

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1980

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     2040

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     2100

gucucugcuu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7522
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7522
gggagaaagc uuaccaugaa ggagaagaag agacggggcg cagauaccag ugucggaauu       60

guuggccucc ugcugaccac agcuauggca gcggagguca cuagacgugg gagugcauac      120

uauauguacu uggacagaaa cgaugcuggg gaggccauau cuuuuccaac cacauugggg      180

augaauaagu guuauauaca gaucauggau cuuggacaca ugugugaugc caccaugagc      240

uaugaaugcc cuaugcugga ugagggggug gaaccagaug acgucgauug uuggugcaac      300

acgacgucaa cuuggguugu guacggaacc ugccaucaca aaaaagguga agcacggaga      360

ucuagaagag cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      420

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      480

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      540

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      600

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      660

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      720

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      780

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      840

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      900

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      960

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca     1020

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag     1080

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu     1140

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau     1200

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca     1260

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1320

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1380

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1440

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1500

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1560

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1620

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1680

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1740

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1920

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1980

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     2040

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     2100

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  7523
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7523
gggagaaagc uuaccaugga gaagaagaga cggggcgcag auaccagugu cggaauuguu       60

ggccuccugc ugaccacagc uauggcagcg gaggucacua gacgugggag ugcauacuau      120

auguacuugg acagaaacga ugcuggggag gccauaucuu uuccaaccac auuggggaug      180

aauaaguguu auauacagau cauggaucuu ggacacaugu gugaugccac caugagcuau      240

gaaugcccua ugcuggauga ggggguggaa ccagaugacg ucgauuguug gugcaacacg      300

acgucaacuu ggguugugua cggaaccugc caucacaaaa aaggugaagc acggagaucu      360

agaagagcug ugacgcuccc cucccauucc acuaggaagc ugcaaacgcg gucgcaaacc      420

ugguuggaau caagagaaua cacaaagcac uugauuagag ucgaaaauug gauauucagg      480

aacccuggcu ucgcguuagc agcagcugcc aucgcuuggc uuuugggaag cucaacgagc      540

caaaaaguca uauacuuggu caugauacug cugauugccc cggcauacag caucaggugc      600

auaggaguca gcaauaggga cuuuguggaa gguaugucag gugggacuug gguugauauu      660

gucuuggaac auggagguug ugucaccgua auggcacagg acaaaccgac ugucgacaua      720

gagcugguua caacaacagu cagcaacaug gcggagguaa gauccuacug cuaugaggca      780

ucaauaucag acauggcuuc ggacagccgc ugcccaacac aaggugaagc cuaccuugac      840

aagcaaucag acacucaaua ugucugcaaa agaacguuag uggacagagg cuggggaaau      900

ggauguggac uuuuuggcaa agggagucug gugacaugcg cuaaguuugc augcuccaag      960

aaaaugaccg ggaagagcau ccagccagag aaucuggagu accggauaau gcugucaguu     1020

cauggcuccc agcacagugg gaugaucguu aaugacacag gacaugaaac ugaugagaau     1080

agagcgaagg uugagauaac gcccaauuca ccaagagccg aagccacccu gggggguuuu     1140

ggaagccuag gacuugauug ugaaccgagg acaggccuug acuuuucaga uuuguauuac     1200

uugacuauga auaacaagca cugguugguu cacaaggagu gguuccacga cauuccauua     1260

ccuuggcacg cuggggcaga caccggaacu ccacacugga acaacaaaga agcacuggua     1320

gaguucaagg acgcacaugc caaaaggcaa acugucgugg uucuagggag ucaagaagga     1380

gcaguucaca cggcccuugc uggagcucug gaggcugaga uggauggugc aaagggaagg     1440

cuguccucug gccacuugaa augucgccug aaaauggaua aacuuagauu gaagggcgug     1500

ucauacuccu uguguaccgc agcguucaca uucaccaaga ucccggcuga aacacugcac     1560

gggacaguca caguggaggu acaguacgca gggacagaug gaccuugcaa gguuccagcu     1620

cagauggcgg uggacaugca aacucugacc ccaguuggga gguugauaac cgcuaacccc     1680

guaaucacug aaagcacuga gaacucuaag augaugcugg aacuugaucc accauuuggg     1740

gacucuuaca uugucauagg agucggggag aagaagauca cccaccacug gcacaggagu     1800

ggcagcacca uuggaaaagc auuugaagcc acugugagag gugccaagag aauggcaguc     1860

uugggagaca cagccuggga cuuuggauca guuggaggcg cucucaacuc auugggcaag     1920

ggcauccauc aaauuuuugg agcagcuuuc aaaucauugu uuggaggaau guccugguuc     1980

ucacaaauuc ucauuggaac guugcugaug ugguuggguc ugaacacaaa gaauggaucu     2040

auuucccuua ugugcuuggc cuuaggggga guguugaucu ucuuauccac agccgucucu     2100

gcuugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  7524
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7524
gggagaaagc uuaccaugaa gaagagacgg ggcgcagaua ccagugucgg aauuguuggc       60

cuccugcuga ccacagcuau ggcagcggag gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  7525
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7525
gggagaaagc uuaccaugaa gagacggggc gcagauacca gugucggaau uguuggccuc       60

cugcugacca cagcuauggc agcggagguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  7526
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7526
gggagaaagc uuaccaugag acggggcgca gauaccagug ucggaauugu uggccuccug       60

cugaccacag cuauggcagc ggaggucacu agacguggga gugcauacua uauguacuug      120

gacagaaacg augcugggga ggccauaucu uuuccaacca cauuggggau gaauaagugu      180

uauauacaga ucauggaucu uggacacaug ugugaugcca ccaugagcua ugaaugcccu      240

augcuggaug agggggugga accagaugac gucgauuguu ggugcaacac gacgucaacu      300

uggguugugu acggaaccug ccaucacaaa aaaggugaag cacggagauc uagaagagcu      360

gugacgcucc ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa      420

ucaagagaau acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc      480

uucgcguuag cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc      540

auauacuugg ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc      600

agcaauaggg acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa      660

cauggagguu gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu      720

acaacaacag ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca      780

gacauggcuu cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca      840

gacacucaau augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga      900

cuuuuuggca aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc      960

gggaagagca uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc     1020

cagcacagug ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag     1080

guugagauaa cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua     1140

ggacuugauu gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug     1200

aauaacaagc acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac     1260

gcuggggcag acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag     1320

gacgcacaug ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac     1380

acggcccuug cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu     1440

ggccacuuga aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc     1500

uuguguaccg cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc     1560

acaguggagg uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg     1620

guggacaugc aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu     1680

gaaagcacug agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac     1740

auugucauag gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc     1800

auuggaaaag cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac     1860

acagccuggg acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau     1920

caaauuuuug gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu     1980

cucauuggaa cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu     2040

augugcuugg ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcuugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  7527
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7527
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcgga ggucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7528
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7528
gggagaaagc uuaccauggg cgcagauacc agugucggaa uuguuggccu ccugcugacc       60

acagcuaugg cagcggaggu cacuagacgu gggagugcau acuauaugua cuuggacaga      120

aacgaugcug gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua      180

cagaucaugg aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug      240

gaugaggggg uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu      300

guguacggaa ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg      360

cuccccuccc auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga      420

gaauacacaa agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg      480

uuagcagcag cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac      540

uuggucauga uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau      600

agggacuuug uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga      660

gguuguguca ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca      720

acagucagca acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug      780

gcuucggaca gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu      900

ggcaaaggga gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag      960

agcauccagc cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac     1020

agugggauga ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag     1080

auaacgccca auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu     1140

gauugugaac cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac     1200

aagcacuggu ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca     1320

caugccaaaa ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc     1380

cuugcuggag cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac     1440

uugaaauguc gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu     1500

accgcagcgu ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug     1560

gagguacagu acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac     1620

augcaaacuc ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc     1680

acugagaacu cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc     1740

auaggagucg gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga     1800

aaagcauuug aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc     1860

ugggacuuug gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu     1920

uuuggagcag cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu     1980

ggaacguugc ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc     2040

uuggccuuag ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  7529
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7529
gggagaaagc uuaccauggc agauaccagu gucggaauug uuggccuccu gcugaccaca       60

gcuauggcag cggaggucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcuugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  7530
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7530
gggagaaagc uuaccaugga uaccaguguc ggaauuguug gccuccugcu gaccacagcu       60

auggcagcgg aggucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cuugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  7531
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 wild
       type

<400>  7531
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7532
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7532
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggccg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7533
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7533
gggagaaagc uuaccaugcg gggcgcagau accagugucg gaauuguugg ccuccugcug       60

accacagcua uggcagcggc cgucacuaga cgugggagug cauacuauau guacuuggac      120

agaaacgaug cuggggaggc cauaucuuuu ccaaccacau uggggaugaa uaaguguuau      180

auacagauca uggaucuugg acacaugugu gaugccacca ugagcuauga augcccuaug      240

cuggaugagg ggguggaacc agaugacguc gauuguuggu gcaacacgac gucaacuugg      300

guuguguacg gaaccugcca ucacaaaaaa ggugaagcac ggagaucuag aagagcugug      360

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      420

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      480

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      540

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      600

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      660

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      720

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      780

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      840

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      900

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      960

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag     1020

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu     1080

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga     1140

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau     1200

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu     1260

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1320

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1380

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1440

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1500

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1560

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1620

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1680

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1740

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1800

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1860

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1920

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1980

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     2040

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7534
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7534
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg guggagugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7535
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 wild type

<400>  7535
gggagaaagc uuaccaugag aggauugucc ucaaggaaac gccguuccca ugauguucug       60

acugugcaau uccuaauuuu gggaaugcug uugaugacgg gugccgugac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7536
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 3 wild type

<400>  7536
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acggggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7537
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 3 wild type

<400>  7537
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaaucuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7538
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 3 wild type

<400>  7538
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1080

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1140

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1200

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1260

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1320

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1380

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1440

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug     1500

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1560

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1620

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1680

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1740

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1800

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1860

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1920

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1980

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     2040

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2100

gcuguuucug cugacguggg gugcucagug gacuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7539
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 3 wild type

<400>  7539
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7540
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 3 wild type

<400>  7540
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugu caaugauaca     1080

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu     1200

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1380

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1440

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1560

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7541
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 3 wild type

<400>  7541
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggcgca       60

gacaccagca ucggaaucgu uggccuccug uugacuacag ccauggcagc agagaucacu      120

agacguggga gugcauacua cauguacuug gauaggagcg augcagggaa ggccauuucu      180

uucgcuacca cauugggggu gaacaaaugc caugugcaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugagcua ugaaugcccu augcuggacg agggggugga accagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacggcgauc uagaagagcc gucacgcucc caucucacuc cacaaggaaa      420

uugcaaacgc ggucgcagac uuggcuagaa ucaagagaau acacaaagca ccugaucaag      480

guugaaaauu ggauauucag gaacccuggu uuuacgcuag uggcugucgc caucgccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauaggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugacgu uguccuggaa cauggaggcu gcgucaccgu gauggcacag      720

gacaagccaa caguugacau agagcugguc acaacaacgg uuaguaacau ggccgaggug      780

agauccuauu guuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cucuuuggca aagggaguuu ggugacaugu      960

gcuaaguuca cgugcuccaa gaagaugacu gggaagagca uucagccgga gaaccuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcacagug ggaugauugu uaaugaugaa     1080

aacagagcga aggucgaggu uacgcccaau ucaccaagag cagaagcaac ccugggaggc     1140

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau     1200

uaccuaacca ugaauaacaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uugcccuggc augcuggggc agacacugga acuccacauu ggaacaacaa ggaggcauua     1320

guggaauuca aggacgccca cgccaagagg caaaccgucg ugguuuuggg gagccaggaa     1380

ggagccgucc acacggcucu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga     1440

aggcuauucu cuggccacuu gaaaugucgc uuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac cgcggcauuc acauucacca aggucccggc ugaaacacua     1560

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1620

gcccagaugg cgguggacau gcagaccuug accccagucg gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gauaagaaaa ucacccauca cuggcauagg     1800

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggcgcuaa gagaauggca     1860

guccuggggg acacagcuug ggacuuugga ucagucggag guguguuuaa cucauugggc     1920

aagggcauuc aucagauuuu uggagcagcu uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacucugcug gugugguuag gucugaacac aaagaauggg     2040

ucuaucuccc ucacaugcuu agcccugggg ggagugauga ucuuccucuc cacggcuguu     2100

ucugcugacg uggggugcuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7542
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 3 wild type

<400>  7542
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucgcaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugu caaugauaca     1080

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggucuu     1200

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1380

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1440

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1560

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7543
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 3 wild type

<400>  7543
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugacugu caaugauaua     1080

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu     1200

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag acacuggaac uccacacugg     1320

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1380

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1440

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1560

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7544
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 3 wild type

<400>  7544
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaccggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1080

gacgaaaaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1140

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1200

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1260

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1320

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1380

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1440

aagggaaagc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug     1500

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1560

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1620

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1680

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1740

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1800

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1860

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1920

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1980

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     2040

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2100

gcuguuucug cugacguggg gugcucagug gacuucucaa agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7545
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 3 wild type

<400>  7545
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcag gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7546
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 3 wild type

<400>  7546
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaugcuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7547
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 3 wild type

<400>  7547
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuagaaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc cucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7548
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 3 wild type

<400>  7548
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccua cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu gguacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7549
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 3 wild type

<400>  7549
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggagaua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu ggaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcugcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7550
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 3 wild type

<400>  7550
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguucuagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7551
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 3 wild type

<400>  7551
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu acgcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccagcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguc acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcaguacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7552
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 3 wild type

<400>  7552
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu agaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacugau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacuccuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagucgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7553
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 3 wild type

<400>  7553
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucagcu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggccgcaaac cugguuggaa ucaagagaau acacaaagca uuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacaag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7554
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 3 wild type

<400>  7554
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa guucaacgag caaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggga cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7555
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 3 wild type

<400>  7555
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgcg ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu agaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacugau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacuccuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7556
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 3 wild type

<400>  7556
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaagcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca cuaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaauaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacacgc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7557
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 3 wild type

<400>  7557
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuaaug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac uugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacuccaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccagga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7558
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 3 wild type

<400>  7558
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaaacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcc gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggu uucgcuuuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa uguugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7559
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 3 wild type

<400>  7559
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc caccaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca uuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacaag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gccucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cugacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7560
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 3 wild type

<400>  7560
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuaaug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaacu ggauguucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca aggucccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7561
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 3 wild type

<400>  7561
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugagugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauacucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauauuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacucagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gaaucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7562
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 3 wild type

<400>  7562
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu ucaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aacaauauca gacauagcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugccgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7563
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 3 wild type

<400>  7563
gggagaaagc uuaccaugag aauaaucaau gccaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaaucgu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggggguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagcuua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga cucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7564
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 3 wild type

<400>  7564
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc caccaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cugacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accgagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagu uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7565
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 3 wild type

<400>  7565
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaaacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gauagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcc gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

aucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga uaagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ccagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7566
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 3 wild type

<400>  7566
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcgcuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccu ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcauug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucaucggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7567
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 3 wild type

<400>  7567
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug uaaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa aaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuuaggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuuccuuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7568
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 3 wild type

<400>  7568
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgccugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaaa gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7569
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 3 wild type

<400>  7569
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagcuua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gaagcacaug cuaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7570
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 3 wild type

<400>  7570
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacc      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguug      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc aggggcagau     1620

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7571
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 3 wild type

<400>  7571
gggagaaagc uuaccaugag aauaaucaau gcugggaagg agaagaagag acgaggcgca       60

gauacuaaug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac uugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac guucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

aggcugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacuccaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccagga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7572
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 3 wild type

<400>  7572
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaaacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaaag augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcc gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggu uucgcuuuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa uguugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcauaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7573
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 3 wild type

<400>  7573
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc agaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacaugguuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuucucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg agugcugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7574
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 3 wild type

<400>  7574
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuaaug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacau gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugaccugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguccac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag ugguagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaacggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7575
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 3 wild type

<400>  7575
gggagaaagc uuaccaugag aauaaucaau gccaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugcgaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaauac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccgccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaacu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7576
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 3 wild type

<400>  7576
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gaaacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcc gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggu uucgcuuuag cagcagcugc caucgcgugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

cuggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa uguugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ccagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7577
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 3 wild type

<400>  7577
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc cauagcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaauc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucagu augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaauccua ggacuggauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7578
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 3 wild type

<400>  7578
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaagucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cauuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca ugggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7579
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 3 wild type

<400>  7579
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua cauguacuug gacagaaacg augcugggga ggccauaucu      180

uucccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacagagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguu auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggaggcu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacccaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuggauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagau acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuc augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7580
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 3 wild type

<400>  7580
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augccgggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaagcgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cuguugacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagcuu ggugacaugc      960

gcuaaguuug caugcuccaa gaagaugacc gggaagagca uccagccaga gaauuuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa cagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaagag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggu     1920

gcucuuaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7581
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 3 wild type

<400>  7581
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggag ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gauacccaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggaa aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ccauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggac     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcaguacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggc     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7582
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 3 wild type

<400>  7582
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcaca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc cagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc aguaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aacaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac acuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaggcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

guucuuaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc cgcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7583
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 3 wild type

<400>  7583
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagug ucggaauugu uggccuccug cugaccacag cuauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      180

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc cuucccauuc cacuaggaag      420

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgc aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugcucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggc ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag ccaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacagugga cgggacaguc acaguggagg gacaguacgg agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc agacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuauug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggga cguugcugau gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc agguggugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7584
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 3 wild type

<400>  7584
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcaca       60

gauacuagug ucggaauugu uggccuccug cugaccacag ccauggcagc ggaagucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguugg cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucuccaag     1560

aucccggcug aaacacugca cgggacaguc acagaggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc gguaaucacu gaaagcacag agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7585
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 3 wild type

<400>  7585
gggagaaagc uuaccaugag aauaaucaau gccaggaagg agaagaagag acgaggcaca       60

gacacuagug ucggaauugu uggccuccug cugaccacag ccauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccacuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cucuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcu      600

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggcgaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gccaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccgaauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaauucaag gaugcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaggg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc cuguguaccg cagcguucac auucacuaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7586
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 3 wild type

<400>  7586
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcaca       60

gauacuagug ucggaauugu uggccuccug cugaccacag ccauggcagu ggaggucacu      120

agacguggga augcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca caauggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca ccugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug cuugcucuaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugauaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcau gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca gacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucacuaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacuccaa gaugaugcug     1740

gaacuggauc caccauuugg ggacucuuac auugucauag gagucgggga aaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguugggggu     1920

gcucucaacu cacugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcuggu gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7587
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 3 wild type

<400>  7587
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg aaaagaagag acgaggcaca       60

gacacuagug ucggaauugu uggccuccug cugaccacag ccauggcagc ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu augcuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cgcggagauc uagaagagcu gugacgcucc ccucccacuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguugg cagcagcugc caucgcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu cgucuuggaa cauggagguu gugucaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcagaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gccaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccgaauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aagcacuggu agaguucaag gaugcacaug ccaaaaggca aacugucgug     1380

guccuaggga gucaagaggg agcaguucac acggcucuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagac ugaagggcgu gucauacucc cuguguaccg cagcguucac auucacuaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1920

gcucucaacu cauugggcaa ggguauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu gugugcuugg ccuuaggggg aguguugauc     2100

uucuugucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7588
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 3 wild type

<400>  7588
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcaca       60

gauacuagug ucggaauugu uggccuccug cugaccacag ccauggcagu ggaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaacaagugu uacauacaga ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu auguuggaug aggggguaga accagaugac      300

gucgauuguu ggugcaacac gacaucaacu uggguugugu acggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau auacaaagca ccugauuaga      480

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgccugg      540

cuuuugggaa guucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaaaccgg cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuauu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcu     1140

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcau gcuggggcag acaccggaac uccacauugg     1320

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1680

aggcugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaucacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg auuuuggauc aguuggaggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcuggu gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu acgugcuugg ccuuaggggg aguguugauc     2100

uuuuuaucca cagccgucuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7589
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 3 wild type

<400>  7589
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acgaggcgca       60

gauacuagcg ucggaauugu uggccuccuc cugaccacag ccauggcagu agaggucacu      120

agacguggga gugcauacua uauguacuug gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacuggggau gaauaagugu uacauacaaa ucauggaucu uggacacaug      240

ugugaugcca ccaugagcua ugaaugcccu auguuggaug aggggguaga accagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu auggaaccug ccaccacaaa      360

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca ccugauuaga      480

guugaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugu caucgcuugg      540

cuuuugggaa guucaacgag ccaaaaaguc auauaucugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      660

ggugggacuu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      780

agauccuacu gcuaugaggc aucaauaucg gauauggcuu cggacagccg cugcccaaca      840

caaggugagg ccuaccuuga caagcaguca gacacucaau augucugcaa aagaacguua      900

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      960

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggggguuu ugggagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua ccugacuaug aauaacaagc acugguuggu ucacaaggag     1260

ugguuccacg acauuccauu accuuggcau gcuggggcag acacuggaac uccacauugg     1320

aacaacaaag aagcacuggu agaguucaag gacgcacaug caaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1440

auggauggag ccaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1500

aaacuuagau ugaagggcgu gucauacucc uugugcacug cagcguucac auucaccaag     1560

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1620

ggaccuugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc uguaaucacu gaaagcaccg agaacucuaa gaugaugcug     1740

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1800

acccaucacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguugggggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uucggaggaa uguccugguu cucacaaauu cucauuggaa cguugcuggu gugguugggu     2040

cugaauacaa agaauggauc uauuucccuu acgugcuugg ccuuaggggg aguguugauc     2100

uucuuaucca cagccguuuc ugcugaugug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7590
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 3 wild type

<400>  7590
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acguggcgca       60

gacaccagug ucggaauugu uggccuccug cugaccacag ccauggcagu ggaggucacc      120

agacguggga gugcauacua uauguacuua gacagaagcg augcugggga ggccauaucu      180

uuuccaacca cacugggggu gaauaagugu uacauacaga ucauggaucu uggacacaug      240

ugugaugcca caaugagcua ugaaugcccu auguuggaug aggggguaga accagaugac      300

gucgauugcu ggugcaacac gacaucgacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugagg cacggagauc uagaagagcu gugacgcucc ccucucauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugaucaga      480

gucgaaaauu ggauauucag gaacccuggc uuugcguugg cagcagcugc cauugcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu guugauugcc      600

ccggcauaca guaucaggug cauaggaguc agcaauaggg auuuugugga agguauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaagccaa cuguugauau agaguugguc acaacaacgg uuagcaacau ggcggaggua      780

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau auguuugcaa aagaacguua      900

guggacagag guuggggaaa uggaugugga cucuuuggca aagggagccu ggugacaugc      960

gccaaguuug caugcuccaa gaaaaugacu gggaagagca uccagccaga gaaccuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugauugu uaaugacaca     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu gcacaaggag     1260

ugguuccaug acauuccacu accuuggcau gcuggggcag acaccggaac uccacauugg     1320

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agccguucac acggcucuug cuggagcccu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgcuu gaaaauggac     1500

aaacuuagau ugaagggcgu gucauacucc uuauguaccg cggcguucac auucaccaag     1560

aucccggcug aaacgcugca ugggacaguc acaguggagg uacaguaugc agggacagau     1620

ggacccugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc ugugaucacu gaaagcacug agaauucaaa gaugauguug     1740

gaacuugacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaagauc     1800

acccaccacu gguacaggag uggcagcacc aucggaaaag cauuugaagc cacugugaga     1860

ggcgccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc agucggaggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauaggaa cguugcuggu gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu acgugcuugg ccuuaggggg aguguugauc     2100

uuccuaucua cagccgucuc ugcugaugug ggguguucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7591
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 3 wild type

<400>  7591
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaagaagag acguggcgca       60

gacaccagug ucggaauugu uggccuccug cugaccacag ccauggcagu ggaggucacc      120

agacguggga gugcauacua uauguacuua gacagaagcg augcugggaa ggccauaucu      180

uuuccaacca cacugggggu gaauaagugu uacauacaga ucauggaucu uggacacaug      240

ugugaugcca caaugagcua ugaaugcccu auguuggaug aggggguaga accagaugac      300

gucgauugcu ggugcaacac gacaucgacu uggguugugu acggaaccug ccaucacaaa      360

aaaggugagg cacggagauc uagaagagcu gugacgcucc ccucucauuc cacuaggaag      420

cugcaaacgc ggucgcagac cugguuggaa ucaagagaau acacaaagca cuugaucaga      480

gucgaaaauu ggauauucag gaacccuggc uuugcguugg cagcagcugc cauugcuugg      540

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu guugauugcc      600

ccggcauaca guaucaggug cauaggaguc agcaauaggg auuuugugga agguauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      720

gacaagccaa cuguugauau agaguugguc acaacaacgg uuagcaacau ggcggaggua      780

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau auguuugcaa aagaacguua      900

guggacagag guuggggaaa uggaugugga cucuuuggca aagggagccu ggugacaugc      960

gccaaguuug caugcuccaa gaaaaugacu gggaagagca uccagccaga gaaccuggag     1020

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugauugu uaaugacaua     1080

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1140

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1200

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu gcacaaggag     1260

ugguuccaug acauuccacu accuuggcau gcuggggcag acaccggaac uccacauugg     1320

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1380

guucuaggga gucaagaagg agccguucac acggcucuug cuggagcccu ggaggcugag     1440

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgcuu gaaaauggac     1500

aaacuuagau ugaagggcgu gucauacucc uuauguaccg cggcguucac auucaccaag     1560

aucccggcug aaacgcugca ugggacaguc acaguggagg uacaguaugc agggacagau     1620

ggacccugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1680

agguugauaa ccgcuaaccc ugugaucacu gaaagcacug agaauucaaa gaugauguug     1740

gaacuugacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaagauc     1800

acccaccacu ggcacaggag uggcagcacc aucggaaaag cauuugaagc cacugugaga     1860

ggcgccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc agucggaggu     1920

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1980

uuuggaggaa uguccugguu cucacaaauc cucauaggaa cguugcuggu gugguugggu     2040

cugaacacaa agaauggauc uauuucccuu acgugcuugg ccuuaggggg aguguugauc     2100

uuccuaucua cagccgucuc ugcugaugug ggguguucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7592
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 3 wild type

<400>  7592
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggcgca       60

gacaccagca ucggaaucgu uggccuccug uugacuacag ccauggcagc agagaucacu      120

agacguggga gugcauacua cauguacuug gauaggagcg augcagggaa ggccauuucu      180

uucgcuacca cauugggggu gaacaaaugc caugugcaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugagcua ugaaugcccu augcuggacg agggggugga accagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacggcgauc uagaagagcc gucacgcucc caucucacuc cacaaggaaa      420

uugcaaacgc ggucgcagac uuggcuagaa ucaagagaau acacaaagca ccugaucaag      480

guugaaaauu ggauauucag gaacccuggu uuuacgcuag uggcugucgc caucgccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauaggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugacgu uguccuggaa cauggaggcu gcgucaccgu gauggcacag      720

gacaagccaa caguugacau agagcugguc acaacaacgg uuaguaacau ggccgaggug      780

agauccuauu guuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cucuuuggca aagggaguuu ggugacaugu      960

gcuaaguuca cgugcuccaa gaagaugacu gggaagagca uucagccgga gaaccuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcacagug ggaugauugu uaaugacaca     1080

ggacaugaaa cugaugaaaa cagagcgaag gucgagguua cgcccaauuc accaagagca     1140

gaagcaaccc ugggaggcuu uggaagcuua ggacuugauu gugaaccaag gacaggccuu     1200

gacuuuucag aucuguauua ccuaaccaug aauaacaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gcccuggcau gcuggggcag acacuggaac uccacauugg     1320

aacaacaagg aggcauuagu ggaauucaag gacgcccacg ccaagaggca aaccgucgug     1380

guuuugggga gccaggaagg agccguccac acggcucuug cuggagcucu agaggcugag     1440

auggauggug caaagggaag gcuauucucu ggccacuuga aaugucgcuu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcaccg cggcauucac auucaccaag     1560

gucccggcug aaacacuaca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guggacaugc agaccuugac cccagucgga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaauucaaa gaugauguug     1740

gagcucgacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaaaauc     1800

acccaucacu ggcauaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1860

ggcgcuaaga gaauggcagu ccugggggac acagcuuggg acuuuggauc agucggaggu     1920

guguuuaacu cauugggcaa gggcauucau cagauuuuug gagcagcuuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cucugcuggu gugguuaggu     2040

cugaacacaa agaauggguc uaucucccuc acaugcuuag cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7593
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 3 wild type

<400>  7593
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggugca       60

gacaccagca ucggaaucau uggucuccug cugacuacag ccauggcagc ggagauuacc      120

agacguggaa gugcauacua cauguacuug gacaggagcg augcugggaa ggccaucucc      180

uuugcuacca acuugggagu uaacaaaugc cauguacaga ucauggaucu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu auguuggacg agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugaag cacggcgauc uagaagagcu gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca ccugaucaag      480

guugaaaacu ggauauuuag gaaccccggg uuugcgcucg cagcuguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg uuaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauuggaguc agcaauagag acuucgugga aggcauguca      660

ggugggaccu ggguugaugu cgucuuggaa cauggagguu gcgucaccgu uauggcacag      720

gacaagccaa caguugacau agaguugguc acgacaacgg uuaguaacau ggccgaggua      780

agauccuacu guuaugaggc aucaauauca gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga uaagcaauca gacacccaau augugugcaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cuuuuuggca aagggagccu ggugacaugu      960

gccaaguuca cguguuccaa gaagaugacu gggaagagca uucagccgga gaaucuggag     1020

uaucgaauaa ugcuaucagu gcauggcucc cagcacagcg ggaugauugu uaaugacaua     1080

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguca cgcccaauuc accaagagca     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugauu gugaaccaag gacaggccug     1200

gacuuuucag auuuguacua cuugaccaug aacaacaagc acugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag auaccggaac uccacacugg     1320

aacaacaagg aggcauuggu agaguucaaa gaugcccacg ccaagaggca aaccgucguu     1380

guucuaggga gccaggaagg agccguucac acggcucuug cuggagcucu ggaggcugag     1440

auggauggug caaaaggaag gcuguucucc ggccauuuga aaugccgcuu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcaccg cagcguucac auuuaccaag     1560

gucccagcug aaacauugca uggaacaguu acaguggagg ugcaguaugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccagucggg     1680

aggcugauaa ccgccaaccc ugugauuacu gaaagcacug agaauucaaa gaugauguug     1740

gagcucgacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaucacu ggcauaggag ugguagcacc aucggaaaag cauuugaggc cacugugaga     1860

ggugccaaga gaauggcagu ucugggggau acagccuggg acuucggauc agucgggggc     1920

guguucaacu cauugggcaa ggguguucac cagauuuuug gagcagcuuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuggu gugguugggu     2040

uugaacacaa agaauggauc caucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcugucuc ugcugacgug gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7594
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 3 wild type

<400>  7594
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      420

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa ugguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuca cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugu caaugauaua     1080

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguua cgccuaauuc accaagagcg     1140

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu     1200

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag     1260

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg     1320

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc     1380

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag     1440

auggauggug caaagggaag gcuguucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1560

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7595
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 3 wild type

<400>  7595
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggugca       60

gacaccagca ucggaaucau uggucuccug cugacuacag ccauggcagc agagauuacc      120

agacguggaa gugcauacua cauguacuug gacaggagcg augcugggaa ggccaucucc      180

uuugcuacca auuugggagu uaacaaaugc cauguacaga ucauggaucu cgggcacaug      240

ugugacgcca ccaugaguua ugaguguccu auguuggacg agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugaag cacggcgauc uagaagagcu gugacgcucc cuucucacuc uacaaggaag      420

cugcaaacgc ggucgcagac uugguuagaa ucaagagaau acacgaagca cuugaucaag      480

guugaaaacu ggauauucag gaaccccggg uuugcgcucg cagcuguugc cauugccugg      540

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      600

ccggcauaca gcaucaggug cauuggaguc agcaauagag acuucgugga aggcauguca      660

ggugggaccu ggguugaugu cgucuuggaa cauggagguu gcgucaccgu aauggcacag      720

gacaagccaa caguugacau agaguugguc acgacaacgg ucaguaacau ggccgaggua      780

agauccuacu guuaugaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau auguaugcaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cuuuuuggca aagggagccu ggugacaugu      960

gccaaguuca cguguuccaa gaagaugacc gggaagagca uucagccgga gaaucuggag     1020

uaucgaauaa ugcuaucagu gcauggcucc cagcacagcg ggaugauugu uaaugacgaa     1080

aauagggcga aagucgaggu cacgcccaau ucaccaagag cagaagcaac cuugggaggc     1140

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uggacuuuuc agauuuguac     1200

uacuugacca ugaacaauaa gcacugguua gugcacaaag agugguuuca ugacauccca     1260

uugcccuggc augcuggggc agacaccgga acuccacauu ggaacaacaa ggaggcauug     1320

guagaguuca aagaugccca cgcuaagagg caaaccgucg uuguucuagg gagccaggaa     1380

ggagccguuc acacggcucu ugcuggagcu cuggaggcug agauggaugg ugcaaaagga     1440

agguuguucu ccggccauuu gaaaugccgc uuaaaaaugg acaagcuuag auugaagggc     1500

gugucauauu ccuugugcac cgcagcguuc acauucacca aggucccagc ugaaacauug     1560

cauggaacag uuacagugga ggugcaguau gcagggacag auggacccug caagguccca     1620

gcucagaugg cgguggacau gcagacccug accccagucg gaaggcugau aaccgccaac     1680

cccgugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggaguuggg gacaagaaaa uuacccacca cuggcauagg     1800

agugguagca ccaucggaaa agcauuugag gccacuguga gaggugccaa gagaauggca     1860

guucuggggg acacagccug ggacuuugga ucagucgggg gcguguuuaa cucauugggc     1920

aagggcaucc accagauuuu uggagcagcc uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacgcugcug gugugguugg guuugaacac aaagaaugga     2040

uccaucuccc ucacaugcuu ggcccugggg ggagugauga ucuuccucuc cacggcuguc     2100

ucugcugacg uggggugcuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7596
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 3 wild type

<400>  7596
gggagaaagc uuaccaugag aauuaucaau gcuaggaagg agaggaagag acguggcgcu       60

gacaccagca ucggaaucgu cggccuccug cugaccacag ccauggcagc cgagaucacu      120

agacguggga gugcauacua cauguacuug gacaggagcg augcugguaa ggccauuucu      180

uuugccacca cauugggggu gaacaaaugc cauguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccc augcuagacg agggagugga gccagaugac      300

gucgauugcu ggugcaacac gacaucgacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacgacgauc cagaagagcc gugacgcuuc cuucucacuc cacgaggaag      420

cugcaaacgc gaucgcagac uuggcuagag ucaagagaau acacaaagca ccugaucaag      480

guugaaaauu ggauauucag gaaccccggg uuugcgcuag uggcuguagc uauagccugg      540

cuccugggaa gcucgacgag ccaaaaaguu auauacuugg ucaugauauu guugauugcc      600

ccggcauaca gcauuaggug cauaggaguu agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggggguu gcgucaccgu gauggcacag      720

gacaagccaa caguugauau cgaguugguc acgacaacgg uuagcaacau ggccgaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg uuguccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa gagaacauug      900

guggauagag guuggggaaa ugggugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cgugcuccaa gaaaaugaca ggcaagagca uccagccgga gaacuuggag     1020

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacaca     1080

ggacaugaaa cugacgaaaa cagagcaaaa gucgagguca cacccaauuc accaagagca     1140

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu     1200

gacuucucag aucuguauua cuugaccaug aacaauaagc auugguuggu gcacaaggag     1260

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg     1320

aacaacaaag aggcauuggu ggaguucaag gacgcccacg ccaagaggca aacuguugug     1380

guucugggga gccaagaggg agcuguucau acggcccucg cuggagcuuu ggaggcugag     1440

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuaggu ugaagggugu gucauauucc cuguguaccg cagcguucac auuuaccaag     1560

gucccagcug aaacauugca uggaacaguc acaguggagg ugcaguaugc agggacagac     1620

ggacccugca aagucccagc ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaucc ugugaucacu gaaaguacug agaauucaaa gaugauguug     1740

gagcucgacc caccauuugg ggauucuuac auugucauag gagucgggga caagaaaauc     1800

acccaucacu ggcaucggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggggac acagccuggg acuuuggauc aguugggggu     1920

guguuuaauu cauuggguaa ggguauucac cagaucuuug gagcagcuuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cacuguuggu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuuag cccugggggg agugaugauc     2100

uuccuuucca cggcuguuuc ugcugauguu gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7597
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 3 wild type

<400>  7597
gggagaaagc uuaccaugag aauuaucaau gcuaggaagg agaggaagag acguggcgcu       60

gacaccagca ucggaaucgu cggccuccug cugaccacag ccauggcagc cgagaucacc      120

agacguggga gugcauacua cauguacuug gacaggagcg augcugguaa ggccauuucu      180

uuugcuacca cauugggggu gaacaaaugc cauguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccc augcuggacg agggagugga gccagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaacauaaa      360

aaaggugaag cacgacgauc cagaagagcc gugacgcuuc cuucucacuc cacaaggaag      420

cugcaaacgc gaucacagac uuggcuagaa ucaagagaau acacaaagca ccugaucaag      480

guugagaauu ggauauucag gaaccccggg uuugcgcuag uggcuguagc uauugccugg      540

cuccugggaa gcucgacgag ccaaaaaguc auauacuugg uaaugauauu guugauugcc      600

ccggcauaca gcaucaggug cauaggaguu agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggggguu gcgucaccgu gauggcacag      720

gacaagccaa caguugacau cgaguugguc acgacaacgg uuagcaacau ggccgaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cuguccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa gagaacauug      900

guggauagag guuggggaaa ugggugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cgugcuccaa gaaaaugaca ggcaagagca uccagccgga gaacuuggag     1020

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacaua     1080

ggacaugaaa cugacgaaaa cagagcaaaa gucgagguca cacccaauuc accaagagca     1140

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu     1200

gacuucucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaggag     1260

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg     1320

aacaacaaag aggcauuggu ggaguuuaag gacgcccacg ccaagaggca aacuguugug     1380

guucugggga gccaagaggg agcuguucac acggcccucg cuggagcuuu ggaggcugag     1440

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuaggu ugaagggugu gucauauucc cuguguaccg caguguguac cgcagcgaag     1560

gucccagcug aaacauugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guagacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc ugugaucacu gaaaguacug agaauucaaa gaugauguug     1740

gagcucgacc caccauuugg ggauucuuac auugucauag gagucgggga caagaaaauc     1800

acccaucacu ggcaucggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1860

ggugccaaga gaauggcagu cuugggggac acagccuggg acuuuggauc aguugggggu     1920

guguuuaauu cauuggguaa ggguauucac cagaucuuug gagcagcuuu uaaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cacuguuggu gugguuaggu     2040

cugaacacaa agaauggauc uaucucccuc acaugcuuag cccugggggg agugaugauc     2100

uuccuuucca cggcuguuuc ugcugauguu gggugcucgg uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7598
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 3 wild type

<400>  7598
gggagaaagc uuaccaugag aauaaucaau gcuaggaagg agaggaagag acguggagcu       60

gaugccagca ucggaaucgu cagccuccug cugacuacag ucauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gacaggagcg augcugguaa ggccauuucu      180

uucguuacca cacugggggu gaacaaaugc caugugcaga ucauggaccu cgggcauaug      240

ugugacgcca ccaugaguua ugagugcccc augcuggacg agggagugga gccagaugac      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacgacgauc cagaagagcc gugacgcuuc cuucucacuc uacaaggaag      420

uugcaaacgc gaucgcagac uuggcuagaa ucaagagaau acacaaagca ccugaucaag      480

guugagaauu ggauauucag gaaccccggg uuugcgcuag uggcuguagc uauugccugg      540

cuccugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauauu guugauugcc      600

ccggcauaca guaucaggug cauaggaguu agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggagguu gugucaccgu gauggcacag      720

gacaagccaa caguugacau agaguugguc acgacaacgg uuagcaacau ggccgaggug      780

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucuguaa aagaacauug      900

guggacagag guuggggaaa ugggugugga cuuuuuggca aggggagcuu ggugacgugu      960

gccaaguuua caugcuccaa gaaaaugaca gggaagagca uccagccgga gaacuuggag     1020

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacgaa     1080

aacagagcaa aagucgaggu uacacccaau ucaccaagag cagaagcaac cuugggaggu     1140

uuuggaagcc ugggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca     1260

uuaccuuggc auucuggugc agacacugaa acuccacacu ggaacaacaa agaggcacug     1320

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagaa     1380

ggagccguuc acacggcucu cgcuggagcu cuggaggcug agauggaugg ugcgaaggga     1440

aggcuauccu caggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu     1500

gugucauauu cccuguguac cgcagcguuc acauucacca agguuccagc ugaaacauug     1560

cauggaacag ucacagugga ggugcaguau gcagggaggg auggacccug caagguccca     1620

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aacggcuaac     1680

ccugugauca cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1800

agugguagca ucaucggaaa ggcauuugaa gccacuguga gaggcgccaa gagaauggca     1860

gucuugggag acacagccug ggacuuugga ucaguugggg guguguuuaa cucauugggc     1920

aaggguauuc accagaucuu uggagcagcu uucaaaucac uguucggagg aauguccugg     1980

uucucacaga uccucauagg cacacuguug gugugguugg gucugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu ggccuuggga ggagugauga ucuuccuuuc cacggcuguu     2100

ucugcugaug ugggguguuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7599
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 3 wild type

<400>  7599
gggagaaagc uuaccaugag aauuaucaau gcuaggaagg agaggaagag acguggcgcu       60

gacaccagca ucggaaucgu cggccuccug cugaccacag ccauggcagc cgagaucacu      120

agacguggga gugcauacua cauguacuug gacaggagcg augcugguaa ggccauuucu      180

uuugccacca cauugggggu gaacaaaugc cauguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccc augcuagacg agggagugga gccagaugac      300

gucgauugcu ggugcaacac gacaucgacu uggguugugu acggaaccug ucaucauaaa      360

aaaggugaag cacgacgauc cagaagagcc gugacgcuuc cuucucacuc cacgaggaag      420

cugcaaacgc gaucgcagac uuggcuagag ucaagagaau acacaaagca ccugaucaag      480

guugaaaauu ggauauucag gaaccccggg uuugcgcuag uggcuguagc uauagccugg      540

cuccugggaa gcucgacgag ccaaaaaguu auauacuugg ucaugauauu guugauugcc      600

ccggcauaca gcauuaggug cauaggaguu agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggggguu gcgucaccgu gauggcacag      720

gacaagccaa caguugauau cgaguugguc acgacaacgg uuagcaacau ggccgaggua      780

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg uuguccaaca      840

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa gagaacauug      900

guggauagag guuggggaaa ugggugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cgugcuccaa gaaaaugaca ggcaagagca uccagccgga gaacuuggag     1020

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacgaa     1080

aacagagcaa aagucgaggu cacacccaau ucaccaagag cagaagcaac cuugggaggu     1140

uuuggaagcu ugggacuuga cugugaacca aggacaggcc uugacuucuc agaucuguau     1200

uaccugacca ugaacaauaa gcauugguug gugcacaagg agugguuuca ugacauccca     1260

uuaccuuggc augcuggugc agacacugga acuccacacu ggaacaacaa agaggcauug     1320

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagag     1380

ggagcuguuc auacggcccu cgcuggagcu uuggaggcug agauggaugg ugcaaaggga     1440

aggcuauucu cuggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu     1500

gugucauauu cccuguguac cgcagcguuc acauuuacca aggucccagc ugaaacauug     1560

cauggaacag ucacagugga ggugcaguau gcagggacag acggacccug caaaguccca     1620

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aaccgccaau     1680

ccugugauca cugaaaguac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1740

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1800

agugguagca ccaucggaaa ggcauuugaa gccacuguga gaggugccaa gagaauggca     1860

gucuuggggg acacagccug ggacuuugga ucaguugggg guguguuuaa uucauugggu     1920

aaggguauuc accagaucuu uggagcagcu uucaaaucac uguuuggagg aauguccugg     1980

uucucacaga uccucauagg cacacuguug gugugguuag guuugaacac aaagaaugga     2040

ucuaucuccc ucacaugcuu agcccugggg ggagugauga ucuuccuuuc cacggcuguu     2100

ucugcugaug uugggugcuc gguggacuuc ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7600
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 3 wild type

<400>  7600
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugaga cacggcgauc uagaagaucu gugucgcucc guuaucacua uacaaggaag      420

uugcaaacgc ggucgcagac augguuagaa ucaagagaau acaagaagca cuugaucaug      480

gucgaaaacu ggauauucag gaaccccggg uuugccauag uguccguugc cauuaccugg      540

cugaugggaa gcuugacgag ccaaaaaguc auauacuugg ucaugauagu guugauuguc      600

ccggcauaca guaucagcug cauuggaguc agcaauagag acuuagugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggagggu gcguuaccga gauggcacag      720

gacaagccaa caguugacau agaguugguc acgaugacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucguuaucc gacauggcuu cggccagucg uugcccaaca      840

caaggcgaac ccucccucga caagcaauca gacacucaau cuguaugcaa aagaacauua      900

ggagacagag guuggggaaa ugguuguggg auuuuuggca aagggagcuu ggugacaugu      960

uccaaguuca cguguuguaa gaagaugccc gggaagagca uucaaccgga aaaucuggag     1020

uaucggauaa ugcucccagu gcauggcucc cagcauagcg ggaugauugu gaaugacaua     1080

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguca cacccaauuc accaagagca     1140

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu     1200

gacuucucag aucuguauua ucugaccaug aacaacaagc auugguuggu gcacaaggag     1260

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg     1320

aacaacaaag aggcauuggu ggaguucaag gacgcccacg ccaagaggca aacuguugug     1380

guucugggga gccaagaggg agcuguucac acggcccucg cuggagcucu ggaggcugag     1440

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac     1500

aagcuuaggu ugaagggugu gucauauucc cuguguacug cagcguucac auuuaccaag     1560

gucccagcug aaacauugca uggaacaguu acaguggagg ugcagucugc agggacagau     1620

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccaguugga     1680

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1740

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1800

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1860

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc agucgggggu     1920

guguucaacu cacuggguaa gggcauucac cagauuuuug gagcagccuu caaaucacug     1980

uuuggaggaa uguccugguu cucacagauc cucauaggca cgcugcuagu gugguuaggu     2040

uugaacacaa agaauggauc uaucucccuc acaugcuugg cccugggggg agugaugauc     2100

uuccucucca cggcuguuuc ugcugacgug gggugcucag uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7601
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 3 wild type

<400>  7601
gggagaaagc uuaccaugag aauaaucaau gcuaggaaag agaggaagag acguggcgca       60

gacaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc agagaucacu      120

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      180

uuugcuacca caaugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      240

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      300

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      360

aaaggugagg cacggcccuc aagaagagcu gucacgcuuc cuucccauuc cacaagaaag      420

uuacagacac ggucacaaac cuggcuugaa ucacgggagu acacgaagca ccugaucaaa      480

guugagaacu ggauacuccg gaauccaggg uucacaguug cugcucuagc aauggccugg      540

cucuugggaa auuccacgag ccagcgagug aucuaucugg ugaugauccu guugauugcc      600

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      660

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      720

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      780

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      840

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      900

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      960

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga gaauuuggag     1020

uacagaauua ugcugucagu gcacggcucc caacacagcg ggaugauugu gaaugacaua     1080

ggacaugaga ccgaugaaaa cagagcaaaa guggagguca caccaaacuc accaagagcu     1140

gaagcgaccc uagggggcuu cgggaguuug ggacucgauu gugagccaag gaccgguuua     1200

gauuucuccg accuguacua uuuaaccaug aacaacaagc auugguuggu gcacaaggag     1260

ugguuccacg acauuccauu gccauggcac gcuggagcgg acacagggac uccccauugg     1320

aacaacaaag aggcauuggu ugaguucaag gaugcccacg ccaaaagaca gacuguuguc     1380

guguugggaa gucaggaggg ugccguccac acagcccugg caggagcuuu ggaagcggag     1440

auggauggag ccaaaggaag gcuguucuca ggucauuuga agugccggau aaagauggac     1500

aagcuuaggu ugaagggugu cucauacuca uugugcacug ccgccuucac auucucaaag     1560

guuccggcug aaacgcugca ugggacaguc acaguugagg ugcaguaugc gggcagagau     1620

ggaccuugca aggugccagc ccaaauggca guggauaugc agacucuuac cccaguggga     1680

agguugauaa cggcaaaucc caucauaacu gagagcacug aaaacucgag aaugauguug     1740

gagcuggacc cacccuuugg agacucuuac auugucauug ggguuggaga uaagaaaauu     1800

acccaucauu ggcacagaag uggaagcacg auugggaagg cauuugaggc cacugugaga     1860

ggugcaaaaa ggauggcagu ccugggagau accgccuggg auuuugguuc uguuggaggc     1920

guguucaauu cucugggcaa gggaguucau caggucuucg gagcagccuu uaaauccuua     1980

uuuggcggaa uguccugguu uucacaaauu cuuauuggca cacuuuuagu gugguugggc     2040

uugaauacaa aaaauggauc cauuucccuu gcaugucugg cgcuuggagg ggcgaugauc     2100

uuucucucca cggccgucuc ggcagacgug gggugcucag uugacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  7602
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 3 wild type

<400>  7602
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7603
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 3 wild type

<400>  7603
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7604
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 3 wild type

<400>  7604
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagc uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuc aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuua gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7605
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 3 wild type

<400>  7605
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7606
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 3 wild type

<400>  7606
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auugucaaug auacaggaua ugaaacugac gaaaauagag cgaaagucga gguuacgccu     1080

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa gcuuaggacu ugacugugaa     1140

ccaaggacag gccuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaggca uugguagaau ucaaggaugc ccacgccaag     1320

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaagcugu ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug cacugcggca     1500

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagauc ccaguccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaggccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7607
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 3 wild type

<400>  7607
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc gcaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguu gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auugucaaug auacaggaua ugaaacugac gaaaauagag cgaaagucga gguuacgccu     1080

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa gcuuaggacu ugacugugaa     1140

ccaaggacag gucuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaggca uugguagaau ucaaggaugc ccacgccaag     1320

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaagcugu ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug cacugcggca     1500

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagauc ccaguccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaggccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7608
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 3 wild type

<400>  7608
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguu gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

acugucaaug auauaggaua ugaaacugac gaaaauagag cgaaagucga gguuacgccu     1080

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa gcuuaggacu ugacugugaa     1140

ccaaggacag gccuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacu     1260

ggaacuccac acuggaacaa caaagaggca uugguagaau ucaaggaugc ccacgccaag     1320

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaagcugu ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug cacugcggca     1500

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagauc ccaguccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaggccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7609
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 3 wild type

<400>  7609
gggagaaagc uuaccaugac cagcaucgga aucguuggcc uccuguugac uacagccaug       60

gcagcagaga ucacuagacg ugggagugca uacuacaugu acuuggauag gagcgaugca      120

gggaaggcca uuucuuucgc uaccacauug ggggugaaca aaugccaugu gcagaucaug      180

gaccucgggc acauguguga cgccaccaug agcuaugaau gcccuaugcu ggacgagggg      240

guggaaccag augacgucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc auaaaaaagg ugaagcacgg cgaucuagaa gagccgucac gcucccaucu      360

cacuccacaa ggaaauugca aacgcggucg cagacuuggc uagaaucaag agaauacaca      420

aagcaccuga ucaagguuga aaauuggaua uucaggaacc cugguuuuac gcuaguggcu      480

gucgccaucg ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauag gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gacguugucc uggaacaugg aggcugcguc      660

accgugaugg cacaggacaa gccaacaguu gacauagagc uggucacaac aacgguuagu      720

aacauggccg aggugagauc cuauuguuac gaggcaucaa uaucggacau ggcuucggac      780

agucgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguu      840

ugcaaaagaa cauuggugga cagagguugg ggaaaugggu guggacucuu uggcaaaggg      900

aguuugguga caugugcuaa guucacgugc uccaagaaga ugacugggaa gagcauucag      960

ccggagaacc uggaguaucg gauaaugcua ucagugcaug gcucccagca cagugggaug     1020

auuguuaaug augaaaacag agcgaagguc gagguuacgc ccaauucacc aagagcagaa     1080

gcaacccugg gaggcuuugg aagcuuagga cuugauugug aaccaaggac aggccuugac     1140

uuuucagauc uguauuaccu aaccaugaau aacaagcauu gguuggugca caaagagugg     1200

uuucaugaca ucccauugcc cuggcaugcu ggggcagaca cuggaacucc acauuggaac     1260

aacaaggagg cauuagugga auucaaggac gcccacgcca agaggcaaac cgucgugguu     1320

uuggggagcc aggaaggagc cguccacacg gcucuugcug gagcucuaga ggcugagaug     1380

gauggugcaa agggaaggcu auucucuggc cacuugaaau gucgcuuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcaccgcgg cauucacauu caccaagguc     1500

ccggcugaaa cacuacaugg aacagucaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaagg ucccagccca gauggcggug gacaugcaga ccuugacccc agucggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag uuggggauaa gaaaaucacc     1740

caucacuggc auaggagugg cagcaccauu ggaaaagcau uugaagccac ugugagaggc     1800

gcuaagagaa uggcaguccu gggggacaca gcuugggacu uuggaucagu cggaggugug     1860

uuuaacucau ugggcaaggg cauucaucag auuuuuggag cagcuuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacuc ugcuggugug guuaggucug     1980

aacacaaaga augggucuau cucccucaca ugcuuagccc uggggggagu gaugaucuuc     2040

cucuccacgg cuguuucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  7610
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 3 wild type

<400>  7610
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaccg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga aaauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagc uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuc aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaagcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuua gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7611
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 3 wild type

<400>  7611
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaggggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7612
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 3 wild type

<400>  7612
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua gaaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggccucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7613
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 3 wild type

<400>  7613
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccuacugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacugguac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7614
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 3 wild type

<400>  7614
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccacuaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa uaaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga cacgcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7615
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 3 wild type

<400>  7615
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agauaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguggagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagcu     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7616
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 3 wild type

<400>  7616
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu cuagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7617
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 3 wild type

<400>  7617
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuacgcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccagc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc uggucacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca guaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7618
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 3 wild type

<400>  7618
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cugauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu ccuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacaguc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7619
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 3 wild type

<400>  7619
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu cagcuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggccg caaaccuggu uggaaucaag agaauacaca      420

aagcauuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acaagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7620
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 3 wild type

<400>  7620
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaaguuca acgagcaaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau ugggacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7621
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 3 wild type

<400>  7621
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgcgccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cugauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu ccuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7622
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 3 wild type

<400>  7622
gggagaaagc uuaccaugac uagugucgga aucguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg ggguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gcuuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggacucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7623
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 3 wild type

<400>  7623
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacca ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcauuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acaagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagccuc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucugac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7624
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 3 wild type

<400>  7624
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggauag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagccgugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagaaucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugauaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcccaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7625
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 3 wild type

<400>  7625
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugagu gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua cucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua uuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu cagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaauc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7626
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 3 wild type

<400>  7626
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuucaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaacaa uaucagacau agcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ccgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7627
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 3 wild type

<400>  7627
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacca ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucugac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccga gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggaguuacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7628
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 3 wild type

<400>  7628
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc gcuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auauagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc caccuugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cauugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau cggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7629
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 3 wild type

<400>  7629
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga ugguguaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaaaauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuuag gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu ccuuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7630
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 3 wild type

<400>  7630
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg ccuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaagcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7631
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 3 wild type

<400>  7631
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaaagaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagccgugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cugguuucgc uuuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguu      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaauguug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcau aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7632
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 3 wild type

<400>  7632
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gcuuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggaagc acaugcuaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7633
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 3 wild type

<400>  7633
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acaugugcga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aauacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cgcccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaacucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7634
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 3 wild type

<400>  7634
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucaccagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuggugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcauugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcagggg cagauggacc cugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7635
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 3 wild type

<400>  7635
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagccgugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cugguuucgc uuuagcagca      480

gcugccaucg cguggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugcccuggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaauguug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcccaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7636
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 3 wild type

<400>  7636
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcagagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau gguuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu cucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugc ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7637
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 3 wild type

<400>  7637
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccauag cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gaaucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaguauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa uccuaggacu ggauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7638
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 3 wild type

<400>  7638
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggaag ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccauua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcauggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7639
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 3 wild type

<400>  7639
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuacaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuccc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacag agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aaguuauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg aggcuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ccaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ggauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcc aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagauacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccucaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7640
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 3 wild type

<400>  7640
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcc      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca agcgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auauagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguu gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agcuugguga caugcgcuaa guuugcaugc uccaagaaga ugaccgggaa gagcauccag      960

ccagagaauu uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaacagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aagagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggugcucu uaacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7641
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 3 wild type

<400>  7641
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcagcggaag ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuggcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauucu ccaagauccc ggcugaaaca cugcacggga cagucacaga ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccgguaa ucacugaaag cacagagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa uacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7642
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 3 wild type

<400>  7642
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agauccagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucaguaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaacaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacacugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaagg cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcguucu uaacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa uacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucuccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7643
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 3 wild type

<400>  7643
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggagucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagauac ccaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggaaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguccaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggacaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc cugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca guaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggccugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7644
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 3 wild type

<400>  7644
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcaguggagg ucacuagacg ugggaaugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacaaug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcaccuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcuugc ucuaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug auacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcaugcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcagacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca cuaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

uccaagauga ugcuggaacu ggauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggaaaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug ggggugcucu caacucacug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugguguggu ugggucugaa uacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7645
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 3 wild type

<400>  7645
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcgcgg agaucuagaa gagcugugac gcuccccucc      360

cacuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuggcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auauagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gaugucgucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcag agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgccaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg aauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccuggga gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggaugc acaugccaaa     1320

aggcaaacug ucgugguccu agggagucaa gagggagcag uucacacggc ucuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagacugaag ggcgugucau acucccugug uaccgcagcg     1500

uucacauuca cuaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggua uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa uacaaagaau ggaucuauuu cccuugugug cuuggccuua     2040

gggggagugu ugaucuucuu guccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7646
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 3 wild type

<400>  7646
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcgcgg agaucuagaa gagcugugac gcuccccucc      360

cacuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuggcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auauagcauc aggugcauag gagucaacaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gaugucgucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcag agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgccaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg aauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccuggga gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggaugc acaugccaaa     1320

aggcaaacug ucgugguccu agggagucaa gagggagcag uucacacggc ucuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagacugaag ggcgugucau acucccugug uaccgcagcg     1500

uucacauuca cuaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggua uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa uacaaagaau ggaucuauuu cccuugugug cuuggccuua     2040

gggggagugu ugaucuucuu guccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7647
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 3 wild type

<400>  7647
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagccaug       60

gcaguggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaca aguguuacau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuauguu ggaugagggg      240

guagaaccag augacgucga uuguuggugc aacacgacau caacuugggu uguguacgga      300

accugccacc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauauaca      420

aagcaccuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg ccuggcuuuu gggaaguuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa accggcuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuauugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagcugaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcaugcugg ggcagacacc     1260

ggaacuccac auuggaacaa caaagaagca uugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc cugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggaggcu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ucacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggauuuu     1860

ggaucaguug gaggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugguguggu ugggucugaa uacaaagaau ggaucuauuu cccuuacgug cuuggccuua     2040

gggggagugu ugaucuuuuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7648
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 3 wild type

<400>  7648
gggagaaagc uuaccaugac uagcgucgga auuguuggcc uccuccugac cacagccaug       60

gcaguagagg ucacuagacg ugggagugca uacuauaugu acuuggacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug gggaugaaua aguguuacau acaaaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuauguu ggaugagggg      240

guagaaccag augacgucga uugcuggugc aacacgacau caacuugggu uguguaugga      300

accugccacc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcaccuga uuagaguuga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugucaucg cuuggcuuuu gggaaguuca acgagccaaa aagucauaua ucuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggauau ggcuucggac      780

agccgcugcc caacacaagg ugaggccuac cuugacaagc agucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggga gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuaccuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcaugcugg ggcagacacu     1260

ggaacuccac auuggaacaa caaagaagca cugguagagu ucaaggacgc acaugcaaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggagccaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug cacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga uaugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag caccgagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ucacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug ggggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguucgg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugguguggu ugggucugaa uacaaagaau ggaucuauuu cccuuacgug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7649
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 3 wild type

<400>  7649
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagccaug       60

gcaguggagg ucaccagacg ugggagugca uacuauaugu acuuagacag aagcgaugcu      120

ggggaggcca uaucuuuucc aaccacacug ggggugaaua aguguuacau acagaucaug      180

gaucuuggac acauguguga ugccacaaug agcuaugaau gcccuauguu ggaugagggg      240

guagaaccag augacgucga uugcuggugc aacacgacau cgacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaggcacgg agaucuagaa gagcugugac gcuccccucu      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga ucagagucga aaauuggaua uucaggaacc cuggcuuugc guuggcagca      480

gcugccauug cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacuguuga uugccccggc auacaguauc aggugcauag gagucagcaa uagggauuuu      600

guggaaggua ugucaggugg gaccuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa gccaacuguu gauauagagu uggucacaac aacgguuagc      720

aacauggcgg agguaagauc cuacugcuac gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguu      840

ugcaaaagaa cguuagugga cagagguugg ggaaauggau guggacucuu uggcaaaggg      900

agccugguga caugcgccaa guuugcaugc uccaagaaaa ugacugggaa gagcauccag      960

ccagagaacc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

auuguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccuggga gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcauugg     1200

uuggugcaca aggagugguu ccaugacauu ccacuaccuu ggcaugcugg ggcagacacc     1260

ggaacuccac auuggaacaa caaagaagca uugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagccg uucacacggc ucuugcugga     1380

gcccuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgcuugaaaa uggacaaacu uagauugaag ggcgugucau acuccuuaug uaccgcggcg     1500

uucacauuca ccaagauccc ggcugaaacg cugcauggga cagucacagu ggagguacag     1560

uaugcaggga cagauggacc cugcaagguu ccagcucaga uggcggugga uaugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguga ucacugaaag cacugagaau     1680

ucaaagauga uguuggaacu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggauaaga agaucaccca ccacugguac aggaguggca gcaccaucgg aaaagcauuu     1800

gaagccacug ugagaggcgc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucagucg gaggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau aggaacguug     1980

cugguguggu ugggucugaa cacaaagaau ggaucuauuu cccuuacgug cuuggccuua     2040

gggggagugu ugaucuuccu aucuacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7650
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 3 wild type

<400>  7650
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagccaug       60

gcaguggagg ucaccagacg ugggagugca uacuauaugu acuuagacag aagcgaugcu      120

gggaaggcca uaucuuuucc aaccacacug ggggugaaua aguguuacau acagaucaug      180

gaucuuggac acauguguga ugccacaaug agcuaugaau gcccuauguu ggaugagggg      240

guagaaccag augacgucga uugcuggugc aacacgacau cgacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaggcacgg agaucuagaa gagcugugac gcuccccucu      360

cauuccacua ggaagcugca aacgcggucg cagaccuggu uggaaucaag agaauacaca      420

aagcacuuga ucagagucga aaauuggaua uucaggaacc cuggcuuugc guuggcagca      480

gcugccauug cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacuguuga uugccccggc auacaguauc aggugcauag gagucagcaa uagggauuuu      600

guggaaggua ugucaggugg gaccuggguu gauguugucu uggaacaugg agguuguguu      660

accguaaugg cacaggacaa gccaacuguu gauauagagu uggucacaac aacgguuagc      720

aacauggcgg agguaagauc cuacugcuac gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguu      840

ugcaaaagaa cguuagugga cagagguugg ggaaauggau guggacucuu uggcaaaggg      900

agccugguga caugcgccaa guuugcaugc uccaagaaaa ugacugggaa gagcauccag      960

ccagagaacc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

auuguuaaug acauaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccuggga gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcauugg     1200

uuggugcaca aggagugguu ccaugacauu ccacuaccuu ggcaugcugg ggcagacacc     1260

ggaacuccac auuggaacaa caaagaagca uugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagccg uucacacggc ucuugcugga     1380

gcccuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgcuugaaaa uggacaaacu uagauugaag ggcgugucau acuccuuaug uaccgcggcg     1500

uucacauuca ccaagauccc ggcugaaacg cugcauggga cagucacagu ggagguacag     1560

uaugcaggga cagauggacc cugcaagguu ccagcucaga uggcggugga uaugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguga ucacugaaag cacugagaau     1680

ucaaagauga uguuggaacu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggauaaga agaucaccca ccacuggcac aggaguggca gcaccaucgg aaaagcauuu     1800

gaagccacug ugagaggcgc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucagucg gaggugcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau aggaacguug     1980

cugguguggu ugggucugaa cacaaagaau ggaucuauuu cccuuacgug cuuggccuua     2040

gggggagugu ugaucuuccu aucuacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7651
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 3 wild type

<400>  7651
gggagaaagc uuaccaugac cagcaucgga aucguuggcc uccuguugac uacagccaug       60

gcagcagaga ucacuagacg ugggagugca uacuacaugu acuuggauag gagcgaugca      120

gggaaggcca uuucuuucgc uaccacauug ggggugaaca aaugccaugu gcagaucaug      180

gaccucgggc acauguguga cgccaccaug agcuaugaau gcccuaugcu ggacgagggg      240

guggaaccag augacgucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc auaaaaaagg ugaagcacgg cgaucuagaa gagccgucac gcucccaucu      360

cacuccacaa ggaaauugca aacgcggucg cagacuuggc uagaaucaag agaauacaca      420

aagcaccuga ucaagguuga aaauuggaua uucaggaacc cugguuuuac gcuaguggcu      480

gucgccaucg ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauag gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gacguugucc uggaacaugg aggcugcguc      660

accgugaugg cacaggacaa gccaacaguu gacauagagc uggucacaac aacgguuagu      720

aacauggccg aggugagauc cuauuguuac gaggcaucaa uaucggacau ggcuucggac      780

agucgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguu      840

ugcaaaagaa cauuggugga cagagguugg ggaaaugggu guggacucuu uggcaaaggg      900

aguuugguga caugugcuaa guucacgugc uccaagaaga ugacugggaa gagcauucag      960

ccggagaacc uggaguaucg gauaaugcua ucagugcaug gcucccagca cagugggaug     1020

auuguuaaug acacaggaca ugaaacugau gaaaacagag cgaaggucga gguuacgccc     1080

aauucaccaa gagcagaagc aacccuggga ggcuuuggaa gcuuaggacu ugauugugaa     1140

ccaaggacag gccuugacuu uucagaucug uauuaccuaa ccaugaauaa caagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugcccu ggcaugcugg ggcagacacu     1260

ggaacuccac auuggaacaa caaggaggca uuaguggaau ucaaggacgc ccacgccaag     1320

aggcaaaccg ucgugguuuu ggggagccag gaaggagccg uccacacggc ucuugcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaggcuau ucucuggcca cuugaaaugu     1440

cgcuuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug caccgcggca     1500

uucacauuca ccaagguccc ggcugaaaca cuacauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagguc ccagcccaga uggcggugga caugcagacc     1620

uugaccccag ucggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaau     1680

ucaaagauga uguuggagcu cgacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggauaaga aaaucaccca ucacuggcau aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggcgc uaagagaaug gcaguccugg gggacacagc uugggacuuu     1860

ggaucagucg gagguguguu uaacucauug ggcaagggca uucaucagau uuuuggagca     1920

gcuuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacucug     1980

cugguguggu uaggucugaa cacaaagaau gggucuaucu cccucacaug cuuagcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7652
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 3 wild type

<400>  7652
gggagaaagc uuaccaugac cagcaucgga aucauugguc uccugcugac uacagccaug       60

gcagcggaga uuaccagacg uggaagugca uacuacaugu acuuggacag gagcgaugcu      120

gggaaggcca ucuccuuugc uaccaacuug ggaguuaaca aaugccaugu acagaucaug      180

gaucucgggc acauguguga cgccaccaug aguuaugagu gcccuauguu ggacgaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaagcacgg cgaucuagaa gagcugugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcaccuga ucaagguuga aaacuggaua uuuaggaacc ccggguuugc gcucgcagcu      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuugguuaug      540

auacugcuga uugccccggc auacagcauc aggugcauug gagucagcaa uagagacuuc      600

guggaaggca ugucaggugg gaccuggguu gaugucgucu uggaacaugg agguugcguc      660

accguuaugg cacaggacaa gccaacaguu gacauagagu uggucacgac aacgguuagu      720

aacauggccg agguaagauc cuacuguuau gaggcaucaa uaucagacau ggcuucggac      780

agucgcugcc caacacaagg ugaagccuac cuugauaagc aaucagacac ccaauaugug      840

ugcaaaagaa cauuggugga cagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agccugguga caugugccaa guucacgugu uccaagaaga ugacugggaa gagcauucag      960

ccggagaauc uggaguaucg aauaaugcua ucagugcaug gcucccagca cagcgggaug     1020

auuguuaaug acauaggaca ugaaacugac gaaaacagag cgaaagucga ggucacgccc     1080

aauucaccaa gagcagaagc aaccuuggga ggcuuuggaa gcuuaggacu ugauugugaa     1140

ccaaggacag gccuggacuu uucagauuug uacuacuuga ccaugaacaa caagcacugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagauacc     1260

ggaacuccac acuggaacaa caaggaggca uugguagagu ucaaagaugc ccacgccaag     1320

aggcaaaccg ucguuguucu agggagccag gaaggagccg uucacacggc ucuugcugga     1380

gcucuggagg cugagaugga uggugcaaaa ggaaggcugu ucuccggcca uuugaaaugc     1440

cgcuuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug caccgcagcg     1500

uucacauuua ccaagguccc agcugaaaca uugcauggaa caguuacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagguc ccagcccaga uggcggugga caugcagacc     1620

cugaccccag ucgggaggcu gauaaccgcc aacccuguga uuacugaaag cacugagaau     1680

ucaaagauga uguuggagcu cgacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ucacuggcau aggaguggua gcaccaucgg aaaagcauuu     1800

gaggccacug ugagaggugc caagagaaug gcaguucugg gggauacagc cugggacuuc     1860

ggaucagucg ggggcguguu caacucauug ggcaagggug uucaccagau uuuuggagca     1920

gcuuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cugguguggu uggguuugaa cacaaagaau ggauccaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7653
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 3 wild type

<400>  7653
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguu gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaaugguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guucacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auugucaaug auauaggaca ugaaacugac gaaaacagag cgaaagucga gguuacgccu     1080

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa gcuuaggacu ugacugugaa     1140

ccaaggacag gccuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaggca uugguagaau ucaaggaugc ccacgccaag     1320

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1380

gcucuagagg cugagaugga uggugcaaag ggaaggcugu ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagauugaag ggcgugucau auuccuugug cacugcggca     1500

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagguc ccagcccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaagccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7654
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 3 wild type

<400>  7654
gggagaaagc uuaccaugac cagcaucgga aucauugguc uccugcugac uacagccaug       60

gcagcagaga uuaccagacg uggaagugca uacuacaugu acuuggacag gagcgaugcu      120

gggaaggcca ucuccuuugc uaccaauuug ggaguuaaca aaugccaugu acagaucaug      180

gaucucgggc acauguguga cgccaccaug aguuaugagu guccuauguu ggacgaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaagcacgg cgaucuagaa gagcugugac gcucccuucu      360

cacucuacaa ggaagcugca aacgcggucg cagacuuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcucgcagcu      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauug gagucagcaa uagagacuuc      600

guggaaggca ugucaggugg gaccuggguu gaugucgucu uggaacaugg agguugcguc      660

accguaaugg cacaggacaa gccaacaguu gacauagagu uggucacgac aacggucagu      720

aacauggccg agguaagauc cuacuguuau gaggcaucaa uaucggacau ggcuucggac      780

agucgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauaugua      840

ugcaaaagaa cauuggugga cagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agccugguga caugugccaa guucacgugu uccaagaaga ugaccgggaa gagcauucag      960

ccggagaauc uggaguaucg aauaaugcua ucagugcaug gcucccagca cagcgggaug     1020

auuguuaaug acgaaaauag ggcgaaaguc gaggucacgc ccaauucacc aagagcagaa     1080

gcaaccuugg gaggcuuugg aagcuuagga cuugauugug aaccaaggac aggccuggac     1140

uuuucagauu uguacuacuu gaccaugaac aauaagcacu gguuagugca caaagagugg     1200

uuucaugaca ucccauugcc cuggcaugcu ggggcagaca ccggaacucc acauuggaac     1260

aacaaggagg cauugguaga guucaaagau gcccacgcua agaggcaaac cgucguuguu     1320

cuagggagcc aggaaggagc cguucacacg gcucuugcug gagcucugga ggcugagaug     1380

gauggugcaa aaggaagguu guucuccggc cauuugaaau gccgcuuaaa aauggacaag     1440

cuuagauuga agggcguguc auauuccuug ugcaccgcag cguucacauu caccaagguc     1500

ccagcugaaa cauugcaugg aacaguuaca guggaggugc aguaugcagg gacagaugga     1560

cccugcaagg ucccagcuca gauggcggug gacaugcaga cccugacccc agucggaagg     1620

cugauaaccg ccaaccccgu gauuacugaa agcacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag uuggggacaa gaaaauuacc     1740

caccacuggc auaggagugg uagcaccauc ggaaaagcau uugaggccac ugugagaggu     1800

gccaagagaa uggcaguucu gggggacaca gccugggacu uuggaucagu cgggggcgug     1860

uuuaacucau ugggcaaggg cauccaccag auuuuuggag cagccuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacgc ugcuggugug guuggguuug     1980

aacacaaaga auggauccau cucccucaca ugcuuggccc uggggggagu gaugaucuuc     2040

cucuccacgg cugucucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  7655
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 3 wild type

<400>  7655
gggagaaagc uuaccaugac cagcaucgga aucgucggcc uccugcugac cacagccaug       60

gcagccgaga ucacuagacg ugggagugca uacuacaugu acuuggacag gagcgaugcu      120

gguaaggcca uuucuuuugc caccacauug ggggugaaca aaugccaugu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gccccaugcu agacgaggga      240

guggagccag augacgucga uugcuggugc aacacgacau cgacuugggu uguguacgga      300

accugucauc auaaaaaagg ugaagcacga cgauccagaa gagccgugac gcuuccuucu      360

cacuccacga ggaagcugca aacgcgaucg cagacuuggc uagagucaag agaauacaca      420

aagcaccuga ucaagguuga aaauuggaua uucaggaacc ccggguuugc gcuaguggcu      480

guagcuauag ccuggcuccu gggaagcucg acgagccaaa aaguuauaua cuuggucaug      540

auauuguuga uugccccggc auacagcauu aggugcauag gaguuagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg ggguugcguc      660

accgugaugg cacaggacaa gccaacaguu gauaucgagu uggucacgac aacgguuagc      720

aacauggccg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccguuguc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguc      840

ugcaagagaa cauuggugga uagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugc uccaagaaaa ugacaggcaa gagcauccag      960

ccggagaacu uggaguaccg gauaaugcua ucagugcaug gaucccagca cagugggaug     1020

auugugaaug acacaggaca ugaaacugac gaaaacagag caaaagucga ggucacaccc     1080

aauucaccaa gagcagaagc aaccuuggga gguuuuggaa gcuugggacu ugacugugaa     1140

ccaaggacag gccuugacuu cucagaucug uauuacuuga ccaugaacaa uaagcauugg     1200

uuggugcaca aggagugguu ucaugacauc ccauuaccuu ggcaugcugg ugcagacacu     1260

ggaacuccac acuggaacaa caaagaggca uugguggagu ucaaggacgc ccacgccaag     1320

aggcaaacug uugugguucu ggggagccaa gagggagcug uucauacggc ccucgcugga     1380

gcuuuggagg cugagaugga uggugcaaag ggaaggcuau ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagguugaag ggugugucau auucccugug uaccgcagcg     1500

uucacauuua ccaagguccc agcugaaaca uugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagacggacc cugcaaaguc ccagcccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aauccuguga ucacugaaag uacugagaau     1680

ucaaagauga uguuggagcu cgacccacca uuuggggauu cuuacauugu cauaggaguc     1740

ggggacaaga aaaucaccca ucacuggcau cggaguggua gcaccaucgg aaaggcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gggacacagc cugggacuuu     1860

ggaucaguug gggguguguu uaauucauug gguaagggua uucaccagau cuuuggagca     1920

gcuuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacacug     1980

uugguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuagcccug     2040

gggggaguga ugaucuuccu uuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7656
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 3 wild type

<400>  7656
gggagaaagc uuaccaugac cagcaucgga aucgucggcc uccugcugac cacagccaug       60

gcagccgaga ucaccagacg ugggagugca uacuacaugu acuuggacag gagcgaugcu      120

gguaaggcca uuucuuuugc uaccacauug ggggugaaca aaugccaugu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gccccaugcu ggacgaggga      240

guggagccag augacgucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucaac auaaaaaagg ugaagcacga cgauccagaa gagccgugac gcuuccuucu      360

cacuccacaa ggaagcugca aacgcgauca cagacuuggc uagaaucaag agaauacaca      420

aagcaccuga ucaagguuga gaauuggaua uucaggaacc ccggguuugc gcuaguggcu      480

guagcuauug ccuggcuccu gggaagcucg acgagccaaa aagucauaua cuugguaaug      540

auauuguuga uugccccggc auacagcauc aggugcauag gaguuagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg ggguugcguc      660

accgugaugg cacaggacaa gccaacaguu gacaucgagu uggucacgac aacgguuagc      720

aacauggccg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcuguc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguc      840

ugcaagagaa cauuggugga uagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugc uccaagaaaa ugacaggcaa gagcauccag      960

ccggagaacu uggaguaccg gauaaugcua ucagugcaug gaucccagca cagugggaug     1020

auugugaaug acauaggaca ugaaacugac gaaaacagag caaaagucga ggucacaccc     1080

aauucaccaa gagcagaagc aaccuuggga gguuuuggaa gcuugggacu ugacugugaa     1140

ccaaggacag gccuugacuu cucagaucug uauuaccuga ccaugaacaa uaagcauugg     1200

uuggugcaca aggagugguu ucaugacauc ccauuaccuu ggcaugcugg ugcagacacu     1260

ggaacuccac acuggaacaa caaagaggca uugguggagu uuaaggacgc ccacgccaag     1320

aggcaaacug uugugguucu ggggagccaa gagggagcug uucacacggc ccucgcugga     1380

gcuuuggagg cugagaugga uggugcaaag ggaaggcuau ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagguugaag ggugugucau auucccugug uaccgcagug     1500

uguaccgcag cgaagguccc agcugaaaca uugcauggaa cagucacagu ggaggugcag     1560

uaugcaggga cagauggacc cugcaagguc ccagcccaga uggcgguaga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aacccuguga ucacugaaag uacugagaau     1680

ucaaagauga uguuggagcu cgacccacca uuuggggauu cuuacauugu cauaggaguc     1740

ggggacaaga aaaucaccca ucacuggcau cggaguggua gcaccaucgg aaaggcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gggacacagc cugggacuuu     1860

ggaucaguug gggguguguu uaauucauug gguaagggua uucaccagau cuuuggagca     1920

gcuuuuaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacacug     1980

uugguguggu uaggucugaa cacaaagaau ggaucuaucu cccucacaug cuuagcccug     2040

gggggaguga ugaucuuccu uuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7657
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 3 wild type

<400>  7657
gggagaaagc uuaccaugac cagcaucgga aucgucggcc uccugcugac cacagccaug       60

gcagccgaga ucacuagacg ugggagugca uacuacaugu acuuggacag gagcgaugcu      120

gguaaggcca uuucuuuugc caccacauug ggggugaaca aaugccaugu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gccccaugcu agacgaggga      240

guggagccag augacgucga uugcuggugc aacacgacau cgacuugggu uguguacgga      300

accugucauc auaaaaaagg ugaagcacga cgauccagaa gagccgugac gcuuccuucu      360

cacuccacga ggaagcugca aacgcgaucg cagacuuggc uagagucaag agaauacaca      420

aagcaccuga ucaagguuga aaauuggaua uucaggaacc ccggguuugc gcuaguggcu      480

guagcuauag ccuggcuccu gggaagcucg acgagccaaa aaguuauaua cuuggucaug      540

auauuguuga uugccccggc auacagcauu aggugcauag gaguuagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg ggguugcguc      660

accgugaugg cacaggacaa gccaacaguu gauaucgagu uggucacgac aacgguuagc      720

aacauggccg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccguuguc caacacaagg ugaagccuac cuugacaagc agucagacac ucaauauguc      840

ugcaagagaa cauuggugga uagagguugg ggaaaugggu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugc uccaagaaaa ugacaggcaa gagcauccag      960

ccggagaacu uggaguaccg gauaaugcua ucagugcaug gaucccagca cagugggaug     1020

auugugaaug acgaaaacag agcaaaaguc gaggucacac ccaauucacc aagagcagaa     1080

gcaaccuugg gagguuuugg aagcuuggga cuugacugug aaccaaggac aggccuugac     1140

uucucagauc uguauuaccu gaccaugaac aauaagcauu gguuggugca caaggagugg     1200

uuucaugaca ucccauuacc uuggcaugcu ggugcagaca cuggaacucc acacuggaac     1260

aacaaagagg cauuggugga guucaaggac gcccacgcca agaggcaaac uguugugguu     1320

cuggggagcc aagagggagc uguucauacg gcccucgcug gagcuuugga ggcugagaug     1380

gauggugcaa agggaaggcu auucucuggc cauuugaaau gccgccuaaa aauggacaag     1440

cuuagguuga aggguguguc auauucccug uguaccgcag cguucacauu uaccaagguc     1500

ccagcugaaa cauugcaugg aacagucaca guggaggugc aguaugcagg gacagacgga     1560

cccugcaaag ucccagccca gauggcggug gacaugcaga cccugacccc aguuggaagg     1620

cugauaaccg ccaauccugu gaucacugaa aguacugaga auucaaagau gauguuggag     1680

cucgacccac cauuugggga uucuuacauu gucauaggag ucggggacaa gaaaaucacc     1740

caucacuggc aucggagugg uagcaccauc ggaaaggcau uugaagccac ugugagaggu     1800

gccaagagaa uggcagucuu gggggacaca gccugggacu uuggaucagu ugggggugug     1860

uuuaauucau uggguaaggg uauucaccag aucuuuggag cagcuuucaa aucacuguuu     1920

ggaggaaugu ccugguucuc acagauccuc auaggcacac uguuggugug guuagguuug     1980

aacacaaaga auggaucuau cucccucaca ugcuuagccc uggggggagu gaugaucuuc     2040

cuuuccacgg cuguuucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  7658
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 3 wild type

<400>  7658
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagcagaga ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugagacacgg cgaucuagaa gaucuguguc gcuccguuau      360

cacuauacaa ggaaguugca aacgcggucg cagacauggu uagaaucaag agaauacaag      420

aagcacuuga ucauggucga aaacuggaua uucaggaacc ccggguuugc cauagugucc      480

guugccauua ccuggcugau gggaagcuug acgagccaaa aagucauaua cuuggucaug      540

auaguguuga uugucccggc auacaguauc agcugcauug gagucagcaa uagagacuua      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg agggugcguu      660

accgagaugg cacaggacaa gccaacaguu gacauagagu uggucacgau gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucgu uauccgacau ggcuucggcc      780

agucguugcc caacacaagg cgaacccucc cucgacaagc aaucagacac ucaaucugua      840

ugcaaaagaa cauuaggaga cagagguugg ggaaaugguu gugggauuuu uggcaaaggg      900

agcuugguga cauguuccaa guucacgugu uguaagaaga ugcccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcuc ccagugcaug gcucccagca uagcgggaug     1020

auugugaaug acauaggaca ugaaacugac gaaaacagag cgaaagucga ggucacaccc     1080

aauucaccaa gagcagaagc aaccuuggga gguuuuggaa gcuugggacu ugacugugaa     1140

ccaaggacag gccuugacuu cucagaucug uauuaucuga ccaugaacaa caagcauugg     1200

uuggugcaca aggagugguu ucaugacauc ccauuaccuu ggcaugcugg ugcagacacu     1260

ggaacuccac acuggaacaa caaagaggca uugguggagu ucaaggacgc ccacgccaag     1320

aggcaaacug uugugguucu ggggagccaa gagggagcug uucacacggc ccucgcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcuau ucucuggcca uuugaaaugc     1440

cgccuaaaaa uggacaagcu uagguugaag ggugugucau auucccugug uacugcagcg     1500

uucacauuua ccaagguccc agcugaaaca uugcauggaa caguuacagu ggaggugcag     1560

ucugcaggga cagauggacc cugcaagguc ccagcccaga uggcggugga caugcagacc     1620

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1680

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1740

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1800

gaagccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1860

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1920

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1980

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     2040

gggggaguga ugaucuuccu cuccacggcu guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7659
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 3 wild type

<400>  7659
gggagaaagc uuaccaugag ugucggaauu guuggccucc ugcugaccac agcuauggca       60

gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgaugcuggg      120

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      180

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      240

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      300

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      360

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      420

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      480

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      540

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      600

gaagguaugu caggugggac uuggguugau guugucuugg aacauggagg uugugucacc      660

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      720

auggcggagg uaagauccua cugcuaugag gcaucaauau cagacauggc uucggacagc      780

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      840

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagc      900

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      960

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc     1020

guuaaugaca caggacauga aacugaugag aauagagcga agguugagau aacgcccaau     1080

ucaccaagag ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg     1140

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug     1200

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1260

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1320

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1380

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1440

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc     1500

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1560

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1620

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1680

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1740

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1800

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1860

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu     1920

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1980

augugguugg gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     2040

ggaguguuga ucuucuuauc cacagccguc ucugcuugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  7660
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 3 wild type

<400>  7660
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaacuuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

uccaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caggagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7661
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 3 wild type

<400>  7661
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaacuggaug uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguc ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7662
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 3 wild type

<400>  7662
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacaugacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucggacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga ccugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uccacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aacccuguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggua gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaac ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7663
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 3 wild type

<400>  7663
gggagaaagc uuaccaugac uagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcggagg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcucccuucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accgcaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguu      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

cucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uuggcucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagccacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca guggacggga cagucacagu ggagggacag     1560

uacggaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcagacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uauuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau ugggacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucagguu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7664
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 3 wild type

<400>  7664
gggagaaagc uuaccauggg agcugaugcc agcaucggaa ucgucagccu ccugcugacu       60

acagucaugg cagcagagau cacuagacgc gggagugcau acuacaugua cuuggacagg      120

agcgaugcug guaaggccau uucuuucguu accacacugg gggugaacaa augccaugug      180

cagaucaugg accucgggca uaugugugac gccaccauga guuaugagug ccccaugcug      240

gacgagggag uggagccaga ugacgucgau ugcuggugca acacgacauc aacuuggguu      300

guguacggaa ccugucauca uaaaaaaggu gaagcacgac gauccagaag agccgugacg      360

cuuccuucuc acucuacaag gaaguugcaa acgcgaucgc agacuuggcu agaaucaaga      420

gaauacacaa agcaccugau caagguugag aauuggauau ucaggaaccc cggguuugcg      480

cuaguggcug uagcuauugc cuggcuccug ggaagcucga cgagccaaaa agucauauac      540

uuggucauga uauuguugau ugccccggca uacaguauca ggugcauagg aguuagcaau      600

agagacuucg uggagggcau gucagguggg accuggguug auguugucuu ggaacaugga      660

gguuguguca ccgugauggc acaggacaag ccaacaguug acauagaguu ggucacgaca      720

acgguuagca acauggccga ggugagaucc uacugcuacg aggcaucaau aucggacaug      780

gcuucggaca gucgcugccc aacacaaggu gaagccuacc uugacaagca gucagacacu      840

caauaugucu guaaaagaac auugguggac agagguuggg gaaaugggug uggacuuuuu      900

ggcaagggga gcuuggugac gugugccaag uuuacaugcu ccaagaaaau gacagggaag      960

agcauccagc cggagaacuu ggaguaccgg auaaugcuau cagugcaugg aucccagcac     1020

agugggauga uugugaauga cgaaaacaga gcaaaagucg agguuacacc caauucacca     1080

agagcagaag caaccuuggg agguuuugga agccugggac uugauuguga accaaggaca     1140

ggccuugacu uuucagaucu guauuaccug accaugaaca auaagcauug guuggugcac     1200

aaagaguggu uucaugacau cccauuaccu uggcauucug gugcagacac ugaaacucca     1260

cacuggaaca acaaagaggc acugguggag uucaaggacg cccacgccaa gaggcaaacu     1320

guugugguuc uggggagcca agaaggagcc guucacacgg cucucgcugg agcucuggag     1380

gcugagaugg auggugcgaa gggaaggcua uccucaggcc auuugaaaug ccgccuaaaa     1440

auggacaagc uuagguugaa ggguguguca uauucccugu guaccgcagc guucacauuc     1500

accaagguuc cagcugaaac auugcaugga acagucacag uggaggugca guaugcaggg     1560

agggauggac ccugcaaggu cccagcccag auggcggugg acaugcagac ccugacccca     1620

guuggaaggc ugauaacggc uaacccugug aucacugaaa gcacugagaa uucaaagaug     1680

auguuggagc ucgacccacc auuuggggau ucuuacauug ucauaggagu cggggacaag     1740

aaaaucaccc aucacuggca ucggaguggu agcaucaucg gaaaggcauu ugaagccacu     1800

gugagaggcg ccaagagaau ggcagucuug ggagacacag ccugggacuu uggaucaguu     1860

gggggugugu uuaacucauu gggcaagggu auucaccaga ucuuuggagc agcuuucaaa     1920

ucacuguucg gaggaauguc cugguucuca cagauccuca uaggcacacu guuggugugg     1980

uugggucuga acacaaagaa uggaucuauc ucccucacau gcuuggccuu gggaggagug     2040

augaucuucc uuuccacggc uguuucugcu ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  7665
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 3 wild type

<400>  7665
gggagaaagc uuaccauggg cgcagacacc agcaucggaa ucauuggccu ccugcugacu       60

acagccaugg cagcagagau cacuagacgc gggagugcau acuacaugua cuuggauagg      120

agcgaugccg ggaaggccau uucguuugcu accacaaugg gagugaacaa gugccacgua      180

cagaucaugg accucgggca caugugugac gccaccauga guuaugagug cccuaugcug      240

gaugagggag uggaaccaga ugaugucgau ugcuggugca acacgacauc aacuuggguu      300

guguacggaa ccugucauca caaaaaaggu gaggcacggc ccucaagaag agcugucacg      360

cuuccuuccc auuccacaag aaaguuacag acacggucac aaaccuggcu ugaaucacgg      420

gaguacacga agcaccugau caaaguugag aacuggauac uccggaaucc aggguucaca      480

guugcugcuc uagcaauggc cuggcucuug ggaaauucca cgagccagcg agugaucuau      540

cuggugauga uccuguugau ugccccggca uacaguauca ggugcauugg agucagcaau      600

agagacuucg uggagggcau gucagguggg accuggguug auguugucuu ggaacaugga      660

ggcugcguua ccgugauggc acaggacaag ccaacagucg acauagaguu ggucacgacg      720

acgguuagua acauggccga gguaagaucc uauugcuacg aggcaucgau aucggacaug      780

gcuucggaca gucguugccc aacacaaggu gaagccuacc uugacaagca aucagacacu      840

caauaugucu gcaaaagaac auuaguggac agagguuggg gaaacgguug uggacuuuuu      900

ggcaaaggga gcuuggugac augugccaag uuuacguguu cuaagaagau gaccgggaag      960

agcauucaac cggagaauuu ggaguacaga auuaugcugu cagugcacgg cucccaacac     1020

agcgggauga uugugaauga cauaggacau gagaccgaug aaaacagagc aaaaguggag     1080

gucacaccaa acucaccaag agcugaagcg acccuagggg gcuucgggag uuugggacuc     1140

gauugugagc caaggaccgg uuuagauuuc uccgaccugu acuauuuaac caugaacaac     1200

aagcauuggu uggugcacaa ggagugguuc cacgacauuc cauugccaug gcacgcugga     1260

gcggacacag ggacucccca uuggaacaac aaagaggcau ugguugaguu caaggaugcc     1320

cacgccaaaa gacagacugu ugucguguug ggaagucagg agggugccgu ccacacagcc     1380

cuggcaggag cuuuggaagc ggagauggau ggagccaaag gaaggcuguu cucaggucau     1440

uugaagugcc ggauaaagau ggacaagcuu agguugaagg gugucucaua cucauugugc     1500

acugccgccu ucacauucuc aaagguuccg gcugaaacgc ugcaugggac agucacaguu     1560

gaggugcagu augcgggcag agauggaccu ugcaaggugc cagcccaaau ggcaguggau     1620

augcagacuc uuaccccagu gggaagguug auaacggcaa aucccaucau aacugagagc     1680

acugaaaacu cgagaaugau guuggagcug gacccacccu uuggagacuc uuacauuguc     1740

auugggguug gagauaagaa aauuacccau cauuggcaca gaaguggaag cacgauuggg     1800

aaggcauuug aggccacugu gagaggugca aaaaggaugg caguccuggg agauaccgcc     1860

ugggauuuug guucuguugg aggcguguuc aauucucugg gcaagggagu ucaucagguc     1920

uucggagcag ccuuuaaauc cuuauuuggc ggaauguccu gguuuucaca aauucuuauu     1980

ggcacacuuu uagugugguu gggcuugaau acaaaaaaug gauccauuuc ccuugcaugu     2040

cuggcgcuug gaggggcgau gaucuuucuc uccacggccg ucucggcaug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  7666
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 3 wild type

<400>  7666
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7667
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 3 wild type

<400>  7667
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7668
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 3 wild type

<400>  7668
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      540

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      600

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      660

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      720

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag      780

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc      840

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca      900

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug      960

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1020

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1080

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1140

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1200

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1260

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1320

auggcagucc ugggggauac agccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  7669
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 3 wild type

<400>  7669
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7670
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 3 wild type

<400>  7670
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugu caaugauaca      540

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg      600

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu      660

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag      720

ugguuucaug acaucccauu gccuuggcau gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc      840

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag      900

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac      960

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1020

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1080

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1140

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1200

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1260

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1320

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7671
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 3 wild type

<400>  7671
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa caguugacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugacugu caaugauaua      540

ggauaugaaa cugacgaaaa uagagcgaaa gucgagguua cgccuaauuc accaagagcg      600

gaagcaaccu ugggaggcuu uggaagcuua ggacuugacu gugaaccaag gacaggccuu      660

gacuuuucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaagag      720

ugguuucaug acaucccauu gccuuggcau gcuggggcag acacuggaac uccacacugg      780

aacaacaaag aggcauuggu agaauucaag gaugcccacg ccaagaggca aaccgucguc      840

guucugggga gccaggaagg agccguucac acggcucucg cuggagcucu agaggcugag      900

auggauggug caaagggaaa gcuguucucu ggccauuuga aaugccgccu aaaaauggac      960

aagcuuagau ugaagggcgu gucauauucc uugugcacug cggcauucac auucaccaag     1020

gucccagcug aaacacugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1080

ggacccugca agaucccagu ccagauggcg guggacaugc agacccugac cccaguugga     1140

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1200

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1260

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaggc cacugugaga     1320

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7672
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 3 wild type

<400>  7672
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauaggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugacgu uguccuggaa cauggaggcu gcgucaccgu gauggcacag      180

gacaagccaa caguugacau agagcugguc acaacaacgg uuaguaacau ggccgaggug      240

agauccuauu guuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacauug      360

guggacagag guuggggaaa ugggugugga cucuuuggca aagggaguuu ggugacaugu      420

gcuaaguuca cgugcuccaa gaagaugacu gggaagagca uucagccgga gaaccuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcacagug ggaugauugu uaaugaugaa      540

aacagagcga aggucgaggu uacgcccaau ucaccaagag cagaagcaac ccugggaggc      600

uuuggaagcu uaggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau      660

uaccuaacca ugaauaacaa gcauugguug gugcacaaag agugguuuca ugacauccca      720

uugcccuggc augcuggggc agacacugga acuccacauu ggaacaacaa ggaggcauua      780

guggaauuca aggacgccca cgccaagagg caaaccgucg ugguuuuggg gagccaggaa      840

ggagccgucc acacggcucu ugcuggagcu cuagaggcug agauggaugg ugcaaaggga      900

aggcuauucu cuggccacuu gaaaugucgc uuaaaaaugg acaagcuuag auugaagggc      960

gugucauauu ccuugugcac cgcggcauuc acauucacca aggucccggc ugaaacacua     1020

cauggaacag ucacagugga ggugcaguau gcagggacag auggacccug caagguccca     1080

gcccagaugg cgguggacau gcagaccuug accccagucg gaaggcugau aaccgccaac     1140

cccgugauua cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1200

ggggauucuu acauugucau aggaguuggg gauaagaaaa ucacccauca cuggcauagg     1260

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggcgcuaa gagaauggca     1320

guccuggggg acacagcuug ggacuuugga ucaugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  7673
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 3 wild type

<400>  7673
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      480

uaccggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      540

gacgaaaaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      600

ggaggcuuug gaagcuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      660

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      720

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag      780

gcauugguag aauucaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc      840

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca      900

aagggaaagc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcuuagauug      960

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1020

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1080

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1140

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1200

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1260

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1320

auggcagucc ugggggauac agccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  7674
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 3 wild type

<400>  7674
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gccucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cugacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7675
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 3 wild type

<400>  7675
gggagaaagc uuaccaugag caaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7676
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 3 wild type

<400>  7676
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu gguacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7677
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 3 wild type

<400>  7677
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaaa gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7678
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 3 wild type

<400>  7678
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaauaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacacgc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7679
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 3 wild type

<400>  7679
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggagaua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu ggaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7680
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 3 wild type

<400>  7680
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacuccaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccagga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7681
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 3 wild type

<400>  7681
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacaugguuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuucucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7682
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 3 wild type

<400>  7682
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaua gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccu ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcauug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7683
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 3 wild type

<400>  7683
gggagaaagc uuaccaugag ccaaaaaguc auauauuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacucagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gaaucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7684
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 3 wild type

<400>  7684
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

cuggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa uguugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ccagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7685
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 3 wild type

<400>  7685
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggggguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagcuua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga cucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7686
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 3 wild type

<400>  7686
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguucuagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7687
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 3 wild type

<400>  7687
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagcuua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gaagcacaug cuaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7688
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 3 wild type

<400>  7688
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccgccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7689
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 3 wild type

<400>  7689
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu ucaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aacaauauca gacauagcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugccgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7690
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 3 wild type

<400>  7690
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucuccaag     1020

aucccggcug aaacacugca cgggacaguc acagaggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc gguaaucacu gaaagcacag agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7691
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 3 wild type

<400>  7691
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cugacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accgagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagu uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7692
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 3 wild type

<400>  7692
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaauc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucagu augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaauccua ggacuggauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7693
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 3 wild type

<400>  7693
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug uaaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa aaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuuaggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7694
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 3 wild type

<400>  7694
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguug      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc aggggcagau     1080

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgccaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7695
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 3 wild type

<400>  7695
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc aguaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aacaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac acuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaggcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7696
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 3 wild type

<400>  7696
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgc aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugcucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggc ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag ccaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacagugga cgggacaguc acaguggagg gacaguacgg agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc agacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7697
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 3 wild type

<400>  7697
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      180

gacaaaccgg cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuauu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcu      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcau gcuggggcag acaccggaac uccacauugg      780

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggacccugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

aggcugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaucacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7698
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 3 wild type

<400>  7698
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaua gcaucaggug cauaggaguc aacaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu cgucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcagaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gccaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccgaauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gaugcacaug ccaaaaggca aacugucgug      840

guccuaggga gucaagaggg agcaguucac acggcucuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagac ugaagggcgu gucauacucc cuguguaccg cagcguucac auucacuaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc uguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7699
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 3 wild type

<400>  7699
gggagaaagc uuaccaugag ccaaaaaguc auauauuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggaggcu guguuaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag uuagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauaucg gauauggcuu cggacagccg cugcccaaca      300

caaggugagg ccuaccuuga caagcaguca gacacucaau augucugcaa aagaacguua      360

guggauagag gcuggggaaa uggaugugga cuuuuuggaa aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaccuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua ccugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcau gcuggggcag acacuggaac uccacauugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacucagau ugaagggugu gucauacucc uugugugcug cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1080

ggaccuugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc uguaaucacu gaaagcaccg agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7700
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 3 wild type

<400>  7700
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu guugauugcc       60

ccggcauaca guaucaggug cauaggaguc agcaauaggg auuuugugga agguauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      180

gacaagccaa cuguugauau agaguugguc acaacaacgg uuagcaacau ggcggaggua      240

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau auguuugcaa aagaacguua      360

guggacagag guuggggaaa uggaugugga cucuuuggca aagggagccu ggugacaugc      420

gccaaguuug caugcuccaa gaaaaugacu gggaagagca uccagccaga gaaccuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugauugu uaaugacaua      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu gcacaaggag      720

ugguuccaug acauuccacu accuuggcau gcuggggcag acaccggaac uccacauugg      780

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agccguucac acggcucuug cuggagcccu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgcuu gaaaauggac      960

aaacuuagau ugaagggcgu gucauacucc uuauguaccg cggcguucac auucaccaag     1020

aucccggcug aaacgcugca ugggacaguc acaguggagg uacaguaugc agggacagau     1080

ggacccugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc ugugaucacu gaaagcacug agaauucaaa gaugauguug     1200

gaacuugacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc aucggaaaag cauuugaagc cacugugaga     1320

ggcgccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7701
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 3 wild type

<400>  7701
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu guugauugcc       60

ccggcauaca guaucaggug cauaggaguc agcaauaggg auuuugugga agguauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggagguu guguuaccgu aauggcacag      180

gacaagccaa cuguugauau agaguugguc acaacaacgg uuagcaacau ggcggaggua      240

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau auguuugcaa aagaacguua      360

guggacagag guuggggaaa uggaugugga cucuuuggca aagggagccu ggugacaugc      420

gccaaguuug caugcuccaa gaaaaugacu gggaagagca uccagccaga gaaccuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugauugu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggagguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc auugguuggu gcacaaggag      720

ugguuccaug acauuccacu accuuggcau gcuggggcag acaccggaac uccacauugg      780

aacaacaaag aagcauuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agccguucac acggcucuug cuggagcccu ggaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgcuu gaaaauggac      960

aaacuuagau ugaagggcgu gucauacucc uuauguaccg cggcguucac auucaccaag     1020

aucccggcug aaacgcugca ugggacaguc acaguggagg uacaguaugc agggacagau     1080

ggacccugca agguuccagc ucagauggcg guggauaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc ugugaucacu gaaagcacug agaauucaaa gaugauguug     1200

gaacuugacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaagauc     1260

acccaccacu gguacaggag uggcagcacc aucggaaaag cauuugaagc cacugugaga     1320

ggcgccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7702
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 3 wild type

<400>  7702
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca guaucaggug cauaggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugacgu uguccuggaa cauggaggcu gcgucaccgu gauggcacag      180

gacaagccaa caguugacau agagcugguc acaacaacgg uuaguaacau ggccgaggug      240

agauccuauu guuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau auguuugcaa aagaacauug      360

guggacagag guuggggaaa ugggugugga cucuuuggca aagggaguuu ggugacaugu      420

gcuaaguuca cgugcuccaa gaagaugacu gggaagagca uucagccgga gaaccuggag      480

uaucggauaa ugcuaucagu gcauggcucc cagcacagug ggaugauugu uaaugacaca      540

ggacaugaaa cugaugaaaa cagagcgaag gucgagguua cgcccaauuc accaagagca      600

gaagcaaccc ugggaggcuu uggaagcuua ggacuugauu gugaaccaag gacaggccuu      660

gacuuuucag aucuguauua ccuaaccaug aauaacaagc auugguuggu gcacaaagag      720

ugguuucaug acaucccauu gcccuggcau gcuggggcag acacuggaac uccacauugg      780

aacaacaagg aggcauuagu ggaauucaag gacgcccacg ccaagaggca aaccgucgug      840

guuuugggga gccaggaagg agccguccac acggcucuug cuggagcucu agaggcugag      900

auggauggug caaagggaag gcuauucucu ggccacuuga aaugucgcuu aaaaauggac      960

aagcuuagau ugaagggcgu gucauauucc uugugcaccg cggcauucac auucaccaag     1020

gucccggcug aaacacuaca uggaacaguc acaguggagg ugcaguaugc agggacagau     1080

ggacccugca aggucccagc ccagauggcg guggacaugc agaccuugac cccagucgga     1140

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaauucaaa gaugauguug     1200

gagcucgacc caccauuugg ggauucuuac auugucauag gaguugggga uaagaaaauc     1260

acccaucacu ggcauaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggcgcuaaga gaauggcagu ccugggggac acagcuuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7703
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 3 wild type

<400>  7703
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg uuaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauuggaguc agcaauagag acuucgugga aggcauguca      120

ggugggaccu ggguugaugu cgucuuggaa cauggagguu gcgucaccgu uauggcacag      180

gacaagccaa caguugacau agaguugguc acgacaacgg uuaguaacau ggccgaggua      240

agauccuacu guuaugaggc aucaauauca gacauggcuu cggacagucg cugcccaaca      300

caaggugaag ccuaccuuga uaagcaauca gacacccaau augugugcaa aagaacauug      360

guggacagag guuggggaaa ugggugugga cuuuuuggca aagggagccu ggugacaugu      420

gccaaguuca cguguuccaa gaagaugacu gggaagagca uucagccgga gaaucuggag      480

uaucgaauaa ugcuaucagu gcauggcucc cagcacagcg ggaugauugu uaaugacaua      540

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguca cgcccaauuc accaagagca      600

gaagcaaccu ugggaggcuu uggaagcuua ggacuugauu gugaaccaag gacaggccug      660

gacuuuucag auuuguacua cuugaccaug aacaacaagc acugguuggu gcacaaagag      720

ugguuucaug acaucccauu gccuuggcau gcuggggcag auaccggaac uccacacugg      780

aacaacaagg aggcauuggu agaguucaaa gaugcccacg ccaagaggca aaccgucguu      840

guucuaggga gccaggaagg agccguucac acggcucuug cuggagcucu ggaggcugag      900

auggauggug caaaaggaag gcuguucucc ggccauuuga aaugccgcuu aaaaauggac      960

aagcuuagau ugaagggcgu gucauauucc uugugcaccg cagcguucac auuuaccaag     1020

gucccagcug aaacauugca uggaacaguu acaguggagg ugcaguaugc agggacagau     1080

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccagucggg     1140

aggcugauaa ccgccaaccc ugugauuacu gaaagcacug agaauucaaa gaugauguug     1200

gagcucgacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1260

acccaucacu ggcauaggag ugguagcacc aucggaaaag cauuugaggc cacugugaga     1320

ggugccaaga gaauggcagu ucugggggau acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7704
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 3 wild type

<400>  7704
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg uaaugauauu guugauugcc       60

ccggcauaca gcaucaggug cauaggaguu agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggggguu gcgucaccgu gauggcacag      180

gacaagccaa caguugacau cgaguugguc acgacaacgg uuagcaacau ggccgaggua      240

agauccuacu gcuaugaggc aucaauaucg gacauggcuu cggacagccg cuguccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau augucugcaa gagaacauug      360

guggauagag guuggggaaa ugggugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cgugcuccaa gaaaaugaca ggcaagagca uccagccgga gaacuuggag      480

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacaua      540

ggacaugaaa cugacgaaaa cagagcaaaa gucgagguca cacccaauuc accaagagca      600

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu      660

gacuucucag aucuguauua ccugaccaug aacaauaagc auugguuggu gcacaaggag      720

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg      780

aacaacaaag aggcauuggu ggaguuuaag gacgcccacg ccaagaggca aacuguugug      840

guucugggga gccaagaggg agcuguucac acggcccucg cuggagcuuu ggaggcugag      900

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac      960

aagcuuaggu ugaagggugu gucauauucc cuguguaccg caguguguac cgcagcgaag     1020

gucccagcug aaacauugca uggaacaguc acaguggagg ugcaguaugc agggacagau     1080

ggacccugca aggucccagc ccagauggcg guagacaugc agacccugac cccaguugga     1140

aggcugauaa ccgccaaccc ugugaucacu gaaaguacug agaauucaaa gaugauguug     1200

gagcucgacc caccauuugg ggauucuuac auugucauag gagucgggga caagaaaauc     1260

acccaucacu ggcaucggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggggac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7705
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 3 wild type

<400>  7705
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauauu guugauugcc       60

ccggcauaca guaucaggug cauaggaguu agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggagguu gugucaccgu gauggcacag      180

gacaagccaa caguugacau agaguugguc acgacaacgg uuagcaacau ggccgaggug      240

agauccuacu gcuacgaggc aucaauaucg gacauggcuu cggacagucg cugcccaaca      300

caaggugaag ccuaccuuga caagcaguca gacacucaau augucuguaa aagaacauug      360

guggacagag guuggggaaa ugggugugga cuuuuuggca aggggagcuu ggugacgugu      420

gccaaguuua caugcuccaa gaaaaugaca gggaagagca uccagccgga gaacuuggag      480

uaccggauaa ugcuaucagu gcauggaucc cagcacagug ggaugauugu gaaugacgaa      540

aacagagcaa aagucgaggu uacacccaau ucaccaagag cagaagcaac cuugggaggu      600

uuuggaagcc ugggacuuga uugugaacca aggacaggcc uugacuuuuc agaucuguau      660

uaccugacca ugaacaauaa gcauugguug gugcacaaag agugguuuca ugacauccca      720

uuaccuuggc auucuggugc agacacugaa acuccacacu ggaacaacaa agaggcacug      780

guggaguuca aggacgccca cgccaagagg caaacuguug ugguucuggg gagccaagaa      840

ggagccguuc acacggcucu cgcuggagcu cuggaggcug agauggaugg ugcgaaggga      900

aggcuauccu caggccauuu gaaaugccgc cuaaaaaugg acaagcuuag guugaagggu      960

gugucauauu cccuguguac cgcagcguuc acauucacca agguuccagc ugaaacauug     1020

cauggaacag ucacagugga ggugcaguau gcagggaggg auggacccug caagguccca     1080

gcccagaugg cgguggacau gcagacccug accccaguug gaaggcugau aacggcuaac     1140

ccugugauca cugaaagcac ugagaauuca aagaugaugu uggagcucga cccaccauuu     1200

ggggauucuu acauugucau aggagucggg gacaagaaaa ucacccauca cuggcaucgg     1260

agugguagca ucaucggaaa ggcauuugaa gccacuguga gaggcgccaa gagaauggca     1320

gucuugggag acacagccug ggacuuugga ucaugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  7706
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 3 wild type

<400>  7706
gggagaaagc uuaccaugag ccaaaaaguc auauacuugg ucaugauagu guugauuguc       60

ccggcauaca guaucagcug cauuggaguc agcaauagag acuuagugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggagggu gcguuaccga gauggcacag      180

gacaagccaa caguugacau agaguugguc acgaugacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucguuaucc gacauggcuu cggccagucg uugcccaaca      300

caaggcgaac ccucccucga caagcaauca gacacucaau cuguaugcaa aagaacauua      360

ggagacagag guuggggaaa ugguuguggg auuuuuggca aagggagcuu ggugacaugu      420

uccaaguuca cguguuguaa gaagaugccc gggaagagca uucaaccgga aaaucuggag      480

uaucggauaa ugcucccagu gcauggcucc cagcauagcg ggaugauugu gaaugacaua      540

ggacaugaaa cugacgaaaa cagagcgaaa gucgagguca cacccaauuc accaagagca      600

gaagcaaccu ugggagguuu uggaagcuug ggacuugacu gugaaccaag gacaggccuu      660

gacuucucag aucuguauua ucugaccaug aacaacaagc auugguuggu gcacaaggag      720

ugguuucaug acaucccauu accuuggcau gcuggugcag acacuggaac uccacacugg      780

aacaacaaag aggcauuggu ggaguucaag gacgcccacg ccaagaggca aacuguugug      840

guucugggga gccaagaggg agcuguucac acggcccucg cuggagcucu ggaggcugag      900

auggauggug caaagggaag gcuauucucu ggccauuuga aaugccgccu aaaaauggac      960

aagcuuaggu ugaagggugu gucauauucc cuguguacug cagcguucac auuuaccaag     1020

gucccagcug aaacauugca uggaacaguu acaguggagg ugcagucugc agggacagau     1080

ggacccugca aggucccagc ccagauggcg guggacaugc agacccugac cccaguugga     1140

aggcugauaa ccgccaaccc cgugauuacu gaaagcacug agaacucaaa gaugauguug     1200

gagcuugacc caccauuugg ggauucuuac auugucauag gaguugggga caagaaaauc     1260

acccaccacu ggcauaggag ugguagcacc aucggaaagg cauuugaagc cacugugaga     1320

ggcgccaaga gaauggcagu ccugggggau acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7707
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 3 wild type

<400>  7707
gggagaaagc uuaccaugcg ccaaaaaguc auauacuugg ucaugauacu gcugauugcc       60

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      120

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      180

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      240

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      360

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      420

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      480

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      540

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      600

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      660

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      720

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg      780

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug      840

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu agaggcugag      900

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau      960

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1020

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacugau     1080

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1140

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1200

gaacuugauc caccauuugg ggacuccuac auugucauag gagucgggga gaagaagauc     1260

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1320

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7708
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 3 wild type

<400>  7708
gggagaaagc uuaccaugag ccagcgagug aucuaucugg ugaugauccu guugauugcc       60

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      120

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      180

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      240

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      300

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      360

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      420

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga gaauuuggag      480

uacagaauua ugcugucagu gcacggcucc caacacagcg ggaugauugu gaaugacaua      540

ggacaugaga ccgaugaaaa cagagcaaaa guggagguca caccaaacuc accaagagcu      600

gaagcgaccc uagggggcuu cgggaguuug ggacucgauu gugagccaag gaccgguuua      660

gauuucuccg accuguacua uuuaaccaug aacaacaagc auugguuggu gcacaaggag      720

ugguuccacg acauuccauu gccauggcac gcuggagcgg acacagggac uccccauugg      780

aacaacaaag aggcauuggu ugaguucaag gaugcccacg ccaaaagaca gacuguuguc      840

guguugggaa gucaggaggg ugccguccac acagcccugg caggagcuuu ggaagcggag      900

auggauggag ccaaaggaag gcuguucuca ggucauuuga agugccggau aaagauggac      960

aagcuuaggu ugaagggugu cucauacuca uugugcacug ccgccuucac auucucaaag     1020

guuccggcug aaacgcugca ugggacaguc acaguugagg ugcaguaugc gggcagagau     1080

ggaccuugca aggugccagc ccaaauggca guggauaugc agacucuuac cccaguggga     1140

agguugauaa cggcaaaucc caucauaacu gagagcacug aaaacucgag aaugauguug     1200

gagcuggacc cacccuuugg agacucuuac auugucauug ggguuggaga uaagaaaauu     1260

acccaucauu ggcacagaag uggaagcacg auugggaagg cauuugaggc cacugugaga     1320

ggugcaaaaa ggauggcagu ccugggagau accgccuggg auuuugguuc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7709
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4804/R4834 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA 
       Design 3 GC

<400>  7709
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7710
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4816/R4838 ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA 
       Design 3 GC

<400>  7710
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacacg      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac cacgagcacc ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacgcgcaag      420

cugcagaccc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccacgag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg     1080

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc     1380

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cccugcugau guggcucggg     2040

cugaacgcca agaacggcuc caucagccug augugccucg cgcugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7711
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4820/R4842 ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 
       3 GC

<400>  7711
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agcggaagcg ccggggcgcc       60

gacaccagca ucgggaucau cggccugcug cucacgaccg ccauggcggc cgagaucacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      180

uucgcgacca cgcugggcgu gaacaagugc cacgugcaga ucauggaccu cgggcacaug      240

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccugc ccucccacag cacccggaag      420

cuccagacgc gcucccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      480

guggagaacu ggaucuuccg gaaccccggc uucgcccucg ucgcgguggc caucgccugg      540

cugcuggggu ccagcaccuc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc      600

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      660

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      840

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      900

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      960

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg     1080

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug     1140

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac     1200

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc     1380

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg     1440

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug     1500

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1620

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1680

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1740

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1860

auggccgugc ucggggacac cgccugggac uucggcagcg ugggcggcgu cuucaacucc     1920

cuggggaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucgggacg cugcuggugu ggcucggccu gaacaccaag     2040

aacggcucca ucagccugac cugccucgcg cugggcggcg ugaugaucuu ccuguccacc     2100

gccgucagcg ccgacguggg gugcuccgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7712
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4868/R5082 ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA 
       Design 3 GC

<400>  7712
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc     1380

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgcggugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7713
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4880/R5081 ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA 
       Design 3 GC

<400>  7713
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7714
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 3 GC

<400>  7714
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7715
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 3 GC

<400>  7715
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccgaga ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguu cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcga agcggauggc cgugcucggg gacaccgccu gggacuucgg cagcgugggc     1860

ggcgucuuca acucccuggg gaaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg ggacgcugcu gguguggcuc     1980

ggccugaaca ccaagaacgg cuccaucagc cugaccugcc ucgcgcuggg cggcgugaug     2040

aucuuccugu ccaccgccgu cagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7716
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4870/R5083 ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA 
       Design 3 GC

<400>  7716
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7717
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4826/R4846 ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA 
       Design 3 GC

<400>  7717
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      180

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      360

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug      840

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1080

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1140

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7718
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R4825/R4845 ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA 
       Design 3 GC

<400>  7718
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7719
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 3 GC

<400>  7719
gggagaaagc uuaccauguc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc       60

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      120

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      180

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      300

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      420

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg      540

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug      600

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac      660

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc      840

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg      900

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug      960

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1080

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1140

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1200

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1260

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1320

auggccgugc ucggggacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  7720
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 3 GC

<400>  7720
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc      840

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7721
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       3 GC

<400>  7721
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuc cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7722
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 3
       GC

<400>  7722
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuc cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7723
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 3 GC

<400>  7723
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccgaga ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguc cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcga agcggauggc cgugcucggg gacaccgccu gggacuucgg cagcgugggc     1860

ggcgucuuca acucccuggg gaaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg ggacgcugcu gguguggcuc     1980

ggccugaaca ccaagaacgg cuccaucagc cugaccugcc ucgcgcuggg cggcgugaug     2040

aucuuccugu ccaccgccgu cagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7724
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F399S) mRNA Design 3 
       GC

<400>  7724
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuc cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7725
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       3 GC

<400>  7725
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucguccagg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7726
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 3
       GC

<400>  7726
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugcagg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7727
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 3 
       GC

<400>  7727
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugcagg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7728
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 GC

<400>  7728
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7729
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 GC

<400>  7729
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7730
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 3 GC

<400>  7730
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccgaga ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguu cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc     1800

aagggcgccc agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg     1860

ggcguguuca acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc     1920

cucuucgggg gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug     1980

ggggugaacg cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug     2040

guguuccugg ccaccaacgu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7731
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 GC

<400>  7731
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7732
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5620 ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3
       GC

<400>  7732
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7733
<211>  2362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7733
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcggcg cggcgccgac       60

accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg      120

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      180

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      240

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      360

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      420

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      480

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      540

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      600

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      660

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      720

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      780

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      840

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      900

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac     1020

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1080

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1140

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1200

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1380

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1440

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1500

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1620

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1680

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1740

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1800

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1860

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1920

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     2040

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2100

cucuccaccg cggugagcgc cgacgugggg ugcuccgucg acuucagcaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2340

ucuuuucaga gccaccagaa uu                                              2362


<210>  7734
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7734
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcggcgcgg cgccgacacc       60

agcgugggga ucgugggccu ccugcugacg accgccaugg cggccgaggu cacccggcgc      120

ggcagcgccu acuacaugua ccucgaccgg aacgacgccg gggaggccau cagcuucccg      180

accacgcugg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      240

gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag cacguggguc guguacggca cgugccacca caagaagggg      360

gaggcccgcc ggucccgccg ggccgugacc cugcccagcc acuccacccg caagcugcag      420

acgcggagcc agaccuggcu cgagucccgc gaguacacca agcaccugau ccgggucgag      480

aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg ccgccaucgc cuggcuccug      540

gggagcucca ccagccagaa ggugaucuac cuggugauga uccuccugau cgcccccgcg      600

uacuccaucc gcugcaucgg cgucagcaac cgggacuucg uggaggggau guccggcggc      660

acgugggugg acaucguccu ggagcacggg ggcugcguga ccgugauggc ccaggacaag      720

cccaccgucg acaucgagcu cgugaccacg accgugagca acauggccga gguccgcagc      780

uacugcuacg aggccuccau cagcgacaug gccuccgaca gccggugccc cacccagggg      840

gaggcguacc uggacaagca guccgacacc caguacgugu gcaagcgcac gcuggucgac      900

cggggcuggg ggaacggcug cggccucuuc gggaagggca gccuggugac cugcgccaag      960

uucgccugcu ccaagaagau gaccgggaag uccauccagc cggagaaccu ggaguaccgc     1020

aucaugcucu ccgugcacgg cagccagcac uccgggauga ucgucaacga caccggccac     1080

gagacggacg agaaccgggc caagguggag aucaccccca acagcccccg cgcggaggcc     1140

acccugggcg gguucggcuc ccuggggcuc gacugcgagc cccggaccgg ccuggacuuc     1200

agcgaccugu acuaccucac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cgcugcccug gcacgccggc gccgacaccg ggaccccgca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcg cacgccaagc gccagaccgu ggugguccug     1380

ggcucccagg agggggccgu gcacacggcc cuggccggcg cgcuggaggc cgagauggac     1440

ggggccaagg gccggcucag cuccggccac cugaagugcc gccugaagau ggacaagcuc     1500

cggcugaagg gggugagcua cucccugugc accgccgccu ucaccuucac caagaucccc     1560

gcggagaccc uccacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccg     1620

ugcaaggucc ccgcccagau ggccguggac augcagaccc ugacgcccgu ggggcgccug     1680

aucaccgcga accccgucau caccgagagc accgagaaca gcaagaugau gcucgagcug     1740

gacccgcccu ucggcgacuc cuacaucgug aucggcgugg gggagaagaa gaucacgcac     1800

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg gcagcguggg cggggcccuc     1920

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1980

ggcauguccu gguucagcca gauccucauc ggcacgcugc ugauguggcu cgggcugaac     2040

accaagaacg gcuccaucag ccugaugugc cucgcccugg gcggcguccu gaucuuccuc     2100

uccaccgcgg ugagcgccga cguggggugc uccgucgacu ucagcaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  7735
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7735
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc ggcgcggcgc cgacaccagc       60

guggggaucg ugggccuccu gcugacgacc gccauggcgg ccgaggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  7736
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7736
gggagaaagc uuaccauggc ccgcaaggag aagaagcggc gcggcgccga caccagcgug       60

gggaucgugg gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc      120

gccuacuaca uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg      180

cugggcauga acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc      240

auguccuacg agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc      360

cgccgguccc gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      420

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      480

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      540

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      600

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      660

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      720

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      780

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      840

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      900

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug     1020

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg     1080

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug     1140

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac     1200

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1380

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1440

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1500

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1560

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1620

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1680

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1740

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1920

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     2040

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     2100

gcggugagcg ccgacguggg gugcuccguc gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7737
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7737
gggagaaagc uuaccaugcg caaggagaag aagcggcgcg gcgccgacac cagcgugggg       60

aucgugggcc uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc      120

uacuacaugu accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug      180

ggcaugaaca agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug      240

uccuacgagu gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc      360

cggucccgcc gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      480

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      540

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      600

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      660

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      780

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      840

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      900

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      960

uccaagaaga ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc     1020

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac     1080

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc     1140

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug     1200

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1380

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1440

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1500

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1560

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1620

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1680

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1740

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1800

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1860

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1920

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1980

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     2040

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     2100

gugagcgccg acguggggug cuccgucgac uucagcaagu gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2220

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  7738
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7738
gggagaaagc uuaccaugaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcuc cgucgacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7739
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7739
gggagaaagc uuaccaugga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgacgugg ggugcuccgu cgacuucagc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  7740
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7740
gggagaaagc uuaccaugaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gacguggggu gcuccgucga cuucagcaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  7741
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7741
gggagaaagc uuaccaugaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu ccgucgacuu cagcaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7742
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7742
gggagaaagc uuaccaugcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacgug     2100

gggugcuccg ucgacuucag caagugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  7743
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7743
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcuccgucg acuucagcaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7744
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7744
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cguggggugc     2100

uccgucgacu ucagcaagug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7745
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7745
gggagaaagc uuaccauggc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugcucc     2100

gucgacuuca gcaagugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7746
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7746
gggagaaagc uuaccaugga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcuccguc     2100

gacuucagca agugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  7747
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7747
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cuccgucgac     2100

uucagcaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7748
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7748
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgcag ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cuccgucgac     2100

uucagcaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7749
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7749
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccgc agucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcuccgucg acuucagcaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7750
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7750
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcggggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  7751
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7751
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcgcagucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcucc gucgacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  7752
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7752
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7753
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7753
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcggcg cggcgccgac       60

accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg      120

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      180

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      240

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      360

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      420

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      480

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      540

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      600

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      660

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      720

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      780

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      840

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      900

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac     1020

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1080

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1140

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1200

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1380

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1440

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1500

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1620

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1680

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1740

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1800

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1860

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1920

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     2040

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2100

cucuccaccg cggugagcgc cgacgugggg ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  7754
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7754
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcggcgcgg cgccgacacc       60

agcgugggga ucgugggccu ccugcugacg accgccaugg cggccgaggu cacccggcgc      120

ggcagcgccu acuacaugua ccucgaccgg aacgacgccg gggaggccau cagcuucccg      180

accacgcugg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      240

gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag cacguggguc guguacggca cgugccacca caagaagggg      360

gaggcccgcc ggucccgccg ggccgugacc cugcccagcc acuccacccg caagcugcag      420

acgcggagcc agaccuggcu cgagucccgc gaguacacca agcaccugau ccgggucgag      480

aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg ccgccaucgc cuggcuccug      540

gggagcucca ccagccagaa ggugaucuac cuggugauga uccuccugau cgcccccgcg      600

uacuccaucc gcugcaucgg cgucagcaac cgggacuucg uggaggggau guccggcggc      660

acgugggugg acaucguccu ggagcacggg ggcugcguga ccgugauggc ccaggacaag      720

cccaccgucg acaucgagcu cgugaccacg accgugagca acauggccga gguccgcagc      780

uacugcuacg aggccuccau cagcgacaug gccuccgaca gccggugccc cacccagggg      840

gaggcguacc uggacaagca guccgacacc caguacgugu gcaagcgcac gcuggucgac      900

cggggcuggg ggaacggcug cggccucuuc gggaagggca gccuggugac cugcgccaag      960

uucgccugcu ccaagaagau gaccgggaag uccauccagc cggagaaccu ggaguaccgc     1020

aucaugcucu ccgugcacgg cagccagcac uccgggauga ucgucaacga caccggccac     1080

gagacggacg agaaccgggc caagguggag aucaccccca acagcccccg cgcggaggcc     1140

acccugggcg gguucggcuc ccuggggcuc gacugcgagc cccggaccgg ccuggacuuc     1200

agcgaccugu acuaccucac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cgcugcccug gcacgccggc gccgacaccg ggaccccgca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcg cacgccaagc gccagaccgu ggugguccug     1380

ggcucccagg agggggccgu gcacacggcc cuggccggcg cgcuggaggc cgagauggac     1440

ggggccaagg gccggcucag cuccggccac cugaagugcc gccugaagau ggacaagcuc     1500

cggcugaagg gggugagcua cucccugugc accgccgccu ucaccuucac caagaucccc     1560

gcggagaccc uccacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccg     1620

ugcaaggucc ccgcccagau ggccguggac augcagaccc ugacgcccgu ggggcgccug     1680

aucaccgcga accccgucau caccgagagc accgagaaca gcaagaugau gcucgagcug     1740

gacccgcccu ucggcgacuc cuacaucgug aucggcgugg gggagaagaa gaucacgcac     1800

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg gcagcguggg cggggcccuc     1920

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1980

ggcauguccu gguucagcca gauccucauc ggcacgcugc ugauguggcu cgggcugaac     2040

accaagaacg gcuccaucag ccugaugugc cucgcccugg gcggcguccu gaucuuccuc     2100

uccaccgcgg ugagcgccga cguggggugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  7755
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7755
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc ggcgcggcgc cgacaccagc       60

guggggaucg ugggccuccu gcugacgacc gccauggcgg ccgaggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7756
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7756
gggagaaagc uuaccauggc ccgcaaggag aagaagcggc gcggcgccga caccagcgug       60

gggaucgugg gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc      120

gccuacuaca uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg      180

cugggcauga acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc      240

auguccuacg agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc      360

cgccgguccc gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      420

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      480

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      540

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      600

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      660

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      720

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      780

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      840

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      900

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug     1020

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg     1080

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug     1140

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac     1200

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1380

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1440

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1500

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1560

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1620

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1680

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1740

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1920

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     2040

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     2100

gcggugagcg ccgacguggg gugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  7757
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7757
gggagaaagc uuaccaugcg caaggagaag aagcggcgcg gcgccgacac cagcgugggg       60

aucgugggcc uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc      120

uacuacaugu accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug      180

ggcaugaaca agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug      240

uccuacgagu gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc      360

cggucccgcc gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      480

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      540

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      600

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      660

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      780

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      840

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      900

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      960

uccaagaaga ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc     1020

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac     1080

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc     1140

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug     1200

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1380

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1440

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1500

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1560

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1620

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1680

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1740

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1800

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1860

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1920

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1980

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     2040

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     2100

gugagcgccg acguggggug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7758
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7758
gggagaaagc uuaccaugaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacg uggggugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7759
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7759
gggagaaagc uuaccaugga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgacgugg ggugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7760
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7760
gggagaaagc uuaccaugaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gacguggggu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  7761
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7761
gggagaaagc uuaccaugaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

guggggugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7762
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7762
gggagaaagc uuaccaugcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacgug     2100

gggugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  7763
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7763
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  7764
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7764
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cguggggugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  7765
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7765
gggagaaagc uuaccauggc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgacgu ggggugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  7766
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7766
gggagaaagc uuaccaugga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacguggg gugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  7767
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7767
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7768
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7768
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgcag ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acguggggug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7769
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7769
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccgc agucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacgugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  7770
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7770
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcggggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7771
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7771
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcgcagucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7772
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7772
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  7773
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7773
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcggcg cggcgccgac       60

accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg      120

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      180

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      240

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      360

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      420

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      480

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      540

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      600

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      660

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      720

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      780

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      840

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      900

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac     1020

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1080

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1140

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1200

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1380

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1440

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1500

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1620

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1680

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1740

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1800

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1860

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1920

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     2040

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2100

cucuccaccg cggugagcgc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  7774
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7774
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcggcgcgg cgccgacacc       60

agcgugggga ucgugggccu ccugcugacg accgccaugg cggccgaggu cacccggcgc      120

ggcagcgccu acuacaugua ccucgaccgg aacgacgccg gggaggccau cagcuucccg      180

accacgcugg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      240

gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag cacguggguc guguacggca cgugccacca caagaagggg      360

gaggcccgcc ggucccgccg ggccgugacc cugcccagcc acuccacccg caagcugcag      420

acgcggagcc agaccuggcu cgagucccgc gaguacacca agcaccugau ccgggucgag      480

aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg ccgccaucgc cuggcuccug      540

gggagcucca ccagccagaa ggugaucuac cuggugauga uccuccugau cgcccccgcg      600

uacuccaucc gcugcaucgg cgucagcaac cgggacuucg uggaggggau guccggcggc      660

acgugggugg acaucguccu ggagcacggg ggcugcguga ccgugauggc ccaggacaag      720

cccaccgucg acaucgagcu cgugaccacg accgugagca acauggccga gguccgcagc      780

uacugcuacg aggccuccau cagcgacaug gccuccgaca gccggugccc cacccagggg      840

gaggcguacc uggacaagca guccgacacc caguacgugu gcaagcgcac gcuggucgac      900

cggggcuggg ggaacggcug cggccucuuc gggaagggca gccuggugac cugcgccaag      960

uucgccugcu ccaagaagau gaccgggaag uccauccagc cggagaaccu ggaguaccgc     1020

aucaugcucu ccgugcacgg cagccagcac uccgggauga ucgucaacga caccggccac     1080

gagacggacg agaaccgggc caagguggag aucaccccca acagcccccg cgcggaggcc     1140

acccugggcg gguucggcuc ccuggggcuc gacugcgagc cccggaccgg ccuggacuuc     1200

agcgaccugu acuaccucac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cgcugcccug gcacgccggc gccgacaccg ggaccccgca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcg cacgccaagc gccagaccgu ggugguccug     1380

ggcucccagg agggggccgu gcacacggcc cuggccggcg cgcuggaggc cgagauggac     1440

ggggccaagg gccggcucag cuccggccac cugaagugcc gccugaagau ggacaagcuc     1500

cggcugaagg gggugagcua cucccugugc accgccgccu ucaccuucac caagaucccc     1560

gcggagaccc uccacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccg     1620

ugcaaggucc ccgcccagau ggccguggac augcagaccc ugacgcccgu ggggcgccug     1680

aucaccgcga accccgucau caccgagagc accgagaaca gcaagaugau gcucgagcug     1740

gacccgcccu ucggcgacuc cuacaucgug aucggcgugg gggagaagaa gaucacgcac     1800

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg gcagcguggg cggggcccuc     1920

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1980

ggcauguccu gguucagcca gauccucauc ggcacgcugc ugauguggcu cgggcugaac     2040

accaagaacg gcuccaucag ccugaugugc cucgcccugg gcggcguccu gaucuuccuc     2100

uccaccgcgg ugagcgccga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7775
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7775
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc ggcgcggcgc cgacaccagc       60

guggggaucg ugggccuccu gcugacgacc gccauggcgg ccgaggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7776
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7776
gggagaaagc uuaccauggc ccgcaaggag aagaagcggc gcggcgccga caccagcgug       60

gggaucgugg gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc      120

gccuacuaca uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg      180

cugggcauga acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc      240

auguccuacg agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc      360

cgccgguccc gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      420

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      480

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      540

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      600

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      660

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      720

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      780

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      840

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      900

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug     1020

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg     1080

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug     1140

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac     1200

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1380

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1440

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1500

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1560

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1620

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1680

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1740

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1920

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     2040

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     2100

gcggugagcg ccgacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7777
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7777
gggagaaagc uuaccaugcg caaggagaag aagcggcgcg gcgccgacac cagcgugggg       60

aucgugggcc uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc      120

uacuacaugu accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug      180

ggcaugaaca agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug      240

uccuacgagu gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc      360

cggucccgcc gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      480

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      540

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      600

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      660

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      780

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      840

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      900

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      960

uccaagaaga ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc     1020

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac     1080

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc     1140

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug     1200

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1380

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1440

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1500

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1560

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1620

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1680

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1740

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1800

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1860

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1920

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1980

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     2040

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     2100

gugagcgccg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  7778
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7778
gggagaaagc uuaccaugaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccgacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7779
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7779
gggagaaagc uuaccaugga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  7780
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7780
gggagaaagc uuaccaugaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  7781
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7781
gggagaaagc uuaccaugaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccgac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  7782
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7782
gggagaaagc uuaccaugcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  7783
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7783
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  7784
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7784
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7785
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7785
gggagaaagc uuaccauggc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  7786
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7786
gggagaaagc uuaccaugga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccgacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  7787
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7787
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  7788
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7788
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgcag ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  7789
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7789
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccgc agucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  7790
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7790
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcggggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7791
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7791
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcgcagucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7792
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7792
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggca gcgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  7793
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7793
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcggcgcgg cgccgacacc       60

agcgugggga ucgugggccu ccugcugacg accgccaugg cggccgaggu cacccggcgc      120

ggcagcgccu acuacaugua ccucgaccgg aacgacgccg gggaggccau cagcuucccg      180

accacgcugg gcaugaacaa gugcuacauc cagaucaugg accuggggca caugugcgac      240

gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag cacguggguc guguacggca cgugccacca caagaagggg      360

gaggcccgcc ggucccgccg ggccgugacc cugcccagcc acuccacccg caagcugcag      420

acgcggagcc agaccuggcu cgagucccgc gaguacacca agcaccugau ccgggucgag      480

aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg ccgccaucgc cuggcuccug      540

gggagcucca ccagccagaa ggugaucuac cuggugauga uccuccugau cgcccccgcg      600

uacuccaucc gcugcaucgg cgucagcaac cgggacuucg uggaggggau guccggcggc      660

acgugggugg acaucguccu ggagcacggg ggcugcguga ccgugauggc ccaggacaag      720

cccaccgucg acaucgagcu cgugaccacg accgugagca acauggccga gguccgcagc      780

uacugcuacg aggccuccau cagcgacaug gccuccgaca gccggugccc cacccagggg      840

gaggcguacc uggacaagca guccgacacc caguacgugu gcaagcgcac gcuggucgac      900

cggggcuggg ggaacggcug cggccucuuc gggaagggca gccuggugac cugcgccaag      960

uucgccugcu ccaagaagau gaccgggaag uccauccagc cggagaaccu ggaguaccgc     1020

aucaugcucu ccgugcacgg cagccagcac uccgggauga ucgucaacga caccggccac     1080

gagacggacg agaaccgggc caagguggag aucaccccca acagcccccg cgcggaggcc     1140

acccugggcg gguucggcuc ccuggggcuc gacugcgagc cccggaccgg ccuggacuuc     1200

agcgaccugu acuaccucac gaugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cgcugcccug gcacgccggc gccgacaccg ggaccccgca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcg cacgccaagc gccagaccgu ggugguccug     1380

ggcucccagg agggggccgu gcacacggcc cuggccggcg cgcuggaggc cgagauggac     1440

ggggccaagg gccggcucag cuccggccac cugaagugcc gccugaagau ggacaagcuc     1500

cggcugaagg gggugagcua cucccugugc accgccgccu ucaccuucac caagaucccc     1560

gcggagaccc uccacggcac ggucaccgug gaggugcagu acgccgggac cgacggcccg     1620

ugcaaggucc ccgcccagau ggccguggac augcagaccc ugacgcccgu ggggcgccug     1680

aucaccgcga accccgucau caccgagagc accgagaaca gcaagaugau gcucgagcug     1740

gacccgcccu ucggcgacuc cuacaucgug aucggcgugg gggagaagaa gaucacgcac     1800

cacuggcacc ggagcggcuc caccaucggg aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg gcagcguggg cggggcccuc     1920

aacucccugg gcaaggggau ccaccagauc uucggcgccg ccuucaagag ccuguucggg     1980

ggcauguccu gguucagcca gauccucauc ggcacgcugc ugauguggcu cgggcugaac     2040

accaagaacg gcuccaucag ccugaugugc cucgcccugg gcggcguccu gaucuuccuc     2100

uccaccgcgg ugagcgccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  7794
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7794
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcggcg cggcgccgac       60

accagcgugg ggaucguggg ccuccugcug acgaccgcca uggcggccga ggucacccgg      120

cgcggcagcg ccuacuacau guaccucgac cggaacgacg ccggggaggc caucagcuuc      180

ccgaccacgc ugggcaugaa caagugcuac auccagauca uggaccuggg gcacaugugc      240

gacgcgacca uguccuacga gugccccaug cucgacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcacgugg gucguguacg gcacgugcca ccacaagaag      360

ggggaggccc gccggucccg ccgggccgug acccugccca gccacuccac ccgcaagcug      420

cagacgcgga gccagaccug gcucgagucc cgcgaguaca ccaagcaccu gauccggguc      480

gagaacugga ucuuccgcaa cccgggcuuc gcccuggccg cggccgccau cgccuggcuc      540

cuggggagcu ccaccagcca gaaggugauc uaccugguga ugauccuccu gaucgccccc      600

gcguacucca uccgcugcau cggcgucagc aaccgggacu ucguggaggg gauguccggc      660

ggcacguggg uggacaucgu ccuggagcac gggggcugcg ugaccgugau ggcccaggac      720

aagcccaccg ucgacaucga gcucgugacc acgaccguga gcaacauggc cgagguccgc      780

agcuacugcu acgaggccuc caucagcgac auggccuccg acagccggug ccccacccag      840

ggggaggcgu accuggacaa gcaguccgac acccaguacg ugugcaagcg cacgcugguc      900

gaccggggcu gggggaacgg cugcggccuc uucgggaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggg aaguccaucc agccggagaa ccuggaguac     1020

cgcaucaugc ucuccgugca cggcagccag cacuccggga ugaucgucaa cgacaccggc     1080

cacgagacgg acgagaaccg ggccaaggug gagaucaccc ccaacagccc ccgcgcggag     1140

gccacccugg gcggguucgg cucccugggg cucgacugcg agccccggac cggccuggac     1200

uucagcgacc uguacuaccu cacgaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccgcugcc cuggcacgcc ggcgccgaca ccgggacccc gcacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcgcacgcca agcgccagac cguggugguc     1380

cugggcuccc aggagggggc cgugcacacg gcccuggccg gcgcgcugga ggccgagaug     1440

gacggggcca agggccggcu cagcuccggc caccugaagu gccgccugaa gauggacaag     1500

cuccggcuga agggggugag cuacucccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgcggaga cccuccacgg cacggucacc guggaggugc aguacgccgg gaccgacggc     1620

ccgugcaagg uccccgccca gauggccgug gacaugcaga cccugacgcc cguggggcgc     1680

cugaucaccg cgaaccccgu caucaccgag agcaccgaga acagcaagau gaugcucgag     1740

cuggacccgc ccuucggcga cuccuacauc gugaucggcg ugggggagaa gaagaucacg     1800

caccacuggc accggagcgg cuccaccauc gggaaggccu ucgaggccac cguccgcggc     1860

gccaagcgga uggccgugcu gggggacacc gcgugggacu ucggcagcgu gggcggggcc     1920

cucaacuccc ugggcaaggg gauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

gggggcaugu ccugguucag ccagauccuc aucggcacgc ugcugaugug gcucgggcug     2040

aacaccaaga acggcuccau cagccugaug ugccucgccc ugggcggcgu ccugaucuuc     2100

cucuccaccg cggugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  7795
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7795
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc ggcgcggcgc cgacaccagc       60

guggggaucg ugggccuccu gcugacgacc gccauggcgg ccgaggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7796
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7796
gggagaaagc uuaccauggc ccgcaaggag aagaagcggc gcggcgccga caccagcgug       60

gggaucgugg gccuccugcu gacgaccgcc auggcggccg aggucacccg gcgcggcagc      120

gccuacuaca uguaccucga ccggaacgac gccggggagg ccaucagcuu cccgaccacg      180

cugggcauga acaagugcua cauccagauc auggaccugg ggcacaugug cgacgcgacc      240

auguccuacg agugccccau gcucgacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcacgug ggucguguac ggcacgugcc accacaagaa gggggaggcc      360

cgccgguccc gccgggccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      420

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      480

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      540

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      600

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      660

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      720

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      780

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      840

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      900

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg gaaguccauc cagccggaga accuggagua ccgcaucaug     1020

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg     1080

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug     1140

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac     1200

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1380

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1440

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1500

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1560

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1620

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1680

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1740

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1920

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     2040

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     2100

gcggugagcg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  7797
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7797
gggagaaagc uuaccaugcg caaggagaag aagcggcgcg gcgccgacac cagcgugggg       60

aucgugggcc uccugcugac gaccgccaug gcggccgagg ucacccggcg cggcagcgcc      120

uacuacaugu accucgaccg gaacgacgcc ggggaggcca ucagcuuccc gaccacgcug      180

ggcaugaaca agugcuacau ccagaucaug gaccuggggc acaugugcga cgcgaccaug      240

uccuacgagu gccccaugcu cgacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcacgugggu cguguacggc acgugccacc acaagaaggg ggaggcccgc      360

cggucccgcc gggccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      480

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      540

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      600

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      660

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      780

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      840

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      900

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      960

uccaagaaga ugaccgggaa guccauccag ccggagaacc uggaguaccg caucaugcuc     1020

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac     1080

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc     1140

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug     1200

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1380

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1440

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1500

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1560

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1620

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1680

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1740

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1800

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1860

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1920

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1980

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     2040

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     2100

gugagcgccu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  7798
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7798
gggagaaagc uuaccaugaa ggagaagaag cggcgcggcg ccgacaccag cguggggauc       60

gugggccucc ugcugacgac cgccauggcg gccgagguca cccggcgcgg cagcgccuac      120

uacauguacc ucgaccggaa cgacgccggg gaggccauca gcuucccgac cacgcugggc      180

augaacaagu gcuacaucca gaucauggac cuggggcaca ugugcgacgc gaccaugucc      240

uacgagugcc ccaugcucga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca cgugggucgu guacggcacg ugccaccaca agaaggggga ggcccgccgg      360

ucccgccggg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      420

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      480

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      540

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      600

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      660

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      720

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      780

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      840

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      900

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      960

aagaagauga ccgggaaguc cauccagccg gagaaccugg aguaccgcau caugcucucc     1020

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag     1080

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg     1140

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac     1200

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1380

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1560

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1680

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1800

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1860

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1980

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     2040

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     2100

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  7799
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7799
gggagaaagc uuaccaugga gaagaagcgg cgcggcgccg acaccagcgu ggggaucgug       60

ggccuccugc ugacgaccgc cauggcggcc gaggucaccc ggcgcggcag cgccuacuac      120

auguaccucg accggaacga cgccggggag gccaucagcu ucccgaccac gcugggcaug      180

aacaagugcu acauccagau cauggaccug gggcacaugu gcgacgcgac cauguccuac      240

gagugcccca ugcucgacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcacgu gggucgugua cggcacgugc caccacaaga agggggaggc ccgccggucc      360

cgccgggccg ugacccugcc cagccacucc acccgcaagc ugcagacgcg gagccagacc      420

uggcucgagu cccgcgagua caccaagcac cugauccggg ucgagaacug gaucuuccgc      480

aacccgggcu ucgcccuggc cgcggccgcc aucgccuggc uccuggggag cuccaccagc      540

cagaagguga ucuaccuggu gaugauccuc cugaucgccc ccgcguacuc cauccgcugc      600

aucggcguca gcaaccggga cuucguggag gggauguccg gcggcacgug gguggacauc      660

guccuggagc acgggggcug cgugaccgug auggcccagg acaagcccac cgucgacauc      720

gagcucguga ccacgaccgu gagcaacaug gccgaggucc gcagcuacug cuacgaggcc      780

uccaucagcg acauggccuc cgacagccgg ugccccaccc agggggaggc guaccuggac      840

aagcaguccg acacccagua cgugugcaag cgcacgcugg ucgaccgggg cugggggaac      900

ggcugcggcc ucuucgggaa gggcagccug gugaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg ggaaguccau ccagccggag aaccuggagu accgcaucau gcucuccgug     1020

cacggcagcc agcacuccgg gaugaucguc aacgacaccg gccacgagac ggacgagaac     1080

cgggccaagg uggagaucac ccccaacagc ccccgcgcgg aggccacccu gggcggguuc     1140

ggcucccugg ggcucgacug cgagccccgg accggccugg acuucagcga ccuguacuac     1200

cucacgauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccgcug     1260

cccuggcacg ccggcgccga caccgggacc ccgcacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcgcacgc caagcgccag accguggugg uccugggcuc ccaggagggg     1380

gccgugcaca cggcccuggc cggcgcgcug gaggccgaga uggacggggc caagggccgg     1440

cucagcuccg gccaccugaa gugccgccug aagauggaca agcuccggcu gaagggggug     1500

agcuacuccc ugugcaccgc cgccuucacc uucaccaaga uccccgcgga gacccuccac     1560

ggcacgguca ccguggaggu gcaguacgcc gggaccgacg gcccgugcaa gguccccgcc     1620

cagauggccg uggacaugca gacccugacg cccguggggc gccugaucac cgcgaacccc     1680

gucaucaccg agagcaccga gaacagcaag augaugcucg agcuggaccc gcccuucggc     1740

gacuccuaca ucgugaucgg cgugggggag aagaagauca cgcaccacug gcaccggagc     1800

ggcuccacca ucgggaaggc cuucgaggcc accguccgcg gcgccaagcg gauggccgug     1860

cugggggaca ccgcguggga cuucggcagc gugggcgggg cccucaacuc ccugggcaag     1920

gggauccacc agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc     1980

agccagaucc ucaucggcac gcugcugaug uggcucgggc ugaacaccaa gaacggcucc     2040

aucagccuga ugugccucgc ccugggcggc guccugaucu uccucuccac cgcggugagc     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  7800
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7800
gggagaaagc uuaccaugaa gaagcggcgc ggcgccgaca ccagcguggg gaucgugggc       60

cuccugcuga cgaccgccau ggcggccgag gucacccggc gcggcagcgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga aguccaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  7801
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7801
gggagaaagc uuaccaugaa gcggcgcggc gccgacacca gcguggggau cgugggccuc       60

cugcugacga ccgccauggc ggccgagguc acccggcgcg gcagcgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaagu ccauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  7802
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7802
gggagaaagc uuaccaugcg gcgcggcgcc gacaccagcg uggggaucgu gggccuccug       60

cugacgaccg ccauggcggc cgaggucacc cggcgcggca gcgccuacua cauguaccuc      120

gaccggaacg acgccgggga ggccaucagc uucccgacca cgcugggcau gaacaagugc      180

uacauccaga ucauggaccu ggggcacaug ugcgacgcga ccauguccua cgagugcccc      240

augcucgacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacg      300

ugggucgugu acggcacgug ccaccacaag aagggggagg cccgccgguc ccgccgggcc      360

gugacccugc ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag      420

ucccgcgagu acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc      480

uucgcccugg ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug      540

aucuaccugg ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc      600

agcaaccggg acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag      660

cacgggggcu gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug      720

accacgaccg ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc      780

gacauggccu ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc      840

gacacccagu acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc      900

cucuucggga agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc      960

gggaagucca uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc     1020

cagcacuccg ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag     1080

guggagauca cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug     1140

gggcucgacu gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac     1260

gccggcgccg acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcgcacg ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac     1380

acggcccugg ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc     1440

ggccaccuga agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc     1560

accguggagg ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc     1620

guggacaugc agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc     1680

gagagcaccg agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac     1740

aucgugaucg gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc     1800

aucgggaagg ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac     1860

accgcguggg acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac     1920

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1980

cucaucggca cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug     2040

augugccucg cccugggcgg cguccugauc uuccucucca ccgcggugag cgccugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  7803
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7803
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccga ggucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7804
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7804
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgugggccu ccugcugacg       60

accgccaugg cggccgaggu cacccggcgc ggcagcgccu acuacaugua ccucgaccgg      120

aacgacgccg gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc      300

guguacggca cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc      360

cugcccagcc acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc      420

gaguacacca agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc      480

cuggccgcgg ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac      540

cuggugauga uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac      600

cgggacuucg uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg      660

ggcugcguga ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg      720

accgugagca acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug      780

gccuccgaca gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc      840

caguacgugu gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc      900

gggaagggca gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag      960

uccauccagc cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac     1020

uccgggauga ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag     1080

aucaccccca acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc     1140

gacugcgagc cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc     1260

gccgacaccg ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg     1320

cacgccaagc gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc     1380

cuggccggcg cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac     1440

cugaagugcc gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc     1500

accgccgccu ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac     1620

augcagaccc ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc     1680

accgagaaca gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug     1740

aucggcgugg gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg     1860

ugggacuucg gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

ggcacgcugc ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc     2040

cucgcccugg gcggcguccu gaucuuccuc uccaccgcgg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  7805
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7805
gggagaaagc uuaccauggc cgacaccagc guggggaucg ugggccuccu gcugacgacc       60

gccauggcgg ccgaggucac ccggcgcggc agcgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagucc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  7806
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7806
gggagaaagc uuaccaugga caccagcgug gggaucgugg gccuccugcu gacgaccgcc       60

auggcggccg aggucacccg gcgcggcagc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaaguccauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg ccugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  7807
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5616 ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 
       3 GC

<400>  7807
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7808
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7808
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgcag ucacccggcg cggcagcgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7809
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7809
gggagaaagc uuaccaugcg cggcgccgac accagcgugg ggaucguggg ccuccugcug       60

acgaccgcca uggcggccgc agucacccgg cgcggcagcg ccuacuacau guaccucgac      120

cggaacgacg ccggggaggc caucagcuuc ccgaccacgc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg gcacaugugc gacgcgacca uguccuacga gugccccaug      240

cucgacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcacgugg      300

gucguguacg gcacgugcca ccacaagaag ggggaggccc gccggucccg ccgggccgug      360

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      480

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      540

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      600

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      660

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      720

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      780

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      840

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      900

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      960

aaguccaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag     1020

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug     1080

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg     1140

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc     1260

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1380

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1440

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1560

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1620

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1680

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1740

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1800

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1860

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1980

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     2040

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  7810
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7810
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcggggucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7811
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 GC

<400>  7811
gggagaaagc uuaccaugcg gggccugagc ucccgcaagc ggcgcagcca cgacgugcuc       60

accguccagu uccugauccu ggggaugcuc cugaugaccg gcgcagucac ccggcgcggc      120

agcgccuacu acauguaccu cgaccggaac gacgccgggg aggccaucag cuucccgacc      180

acgcugggca ugaacaagug cuacauccag aucauggacc uggggcacau gugcgacgcg      240

accauguccu acgagugccc caugcucgac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac gugggucgug uacggcacgu gccaccacaa gaagggggag      360

gcccgccggu cccgccgggc cgugacccug cccagccacu ccacccgcaa gcugcagacg      420

cggagccaga ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac      480

uggaucuucc gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg      540

agcuccacca gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac      600

uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg      660

uggguggaca ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc      720

accgucgaca ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac      780

ugcuacgagg ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag      840

gcguaccugg acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg      900

ggcuggggga acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc      960

gccugcucca agaagaugac cgggaagucc auccagccgg agaaccugga guaccgcauc     1020

augcucuccg ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag     1080

acggacgaga accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc     1140

cugggcgggu ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc     1200

gaccuguacu accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacaucccgc ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc     1380

ucccaggagg gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg     1440

gccaagggcc ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg     1500

cugaaggggg ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg     1560

gagacccucc acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc     1620

aagguccccg cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc     1680

accgcgaacc ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac     1740

ccgcccuucg gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac     1800

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag     1860

cggauggccg ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac     1920

ucccugggca aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1980

auguccuggu ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc     2040

aagaacggcu ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc     2100

accgcgguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  7812
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 3 GC

<400>  7812
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1380

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1620

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1680

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7813
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 3 GC

<400>  7813
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacacg      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac cacgagcacc ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacgcgcaag      420

cugcagaccc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccacgag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg     1080

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc     1380

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cccugcugau guggcucggg     2040

cugaacgcca agaacggcuc caucagccug augugccucg cgcugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7814
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 3 GC

<400>  7814
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agcggaagcg ccggggcgcc       60

gacaccagca ucgggaucau cggccugcug cucacgaccg ccauggcggc cgagaucacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      180

uucgcgacca cgcugggcgu gaacaagugc cacgugcaga ucauggaccu cgggcacaug      240

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccugc ccucccacag cacccggaag      420

cuccagacgc gcucccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      480

guggagaacu ggaucuuccg gaaccccggc uucgcccucg ucgcgguggc caucgccugg      540

cugcuggggu ccagcaccuc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc      600

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      660

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      840

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      900

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      960

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg     1080

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug     1140

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac     1200

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc     1380

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg     1440

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug     1500

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1620

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1680

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1740

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1800

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1860

auggccgugc ucggggacac cgccugggac uucggcagcg ugggcggcgu cuucaacucc     1920

cuggggaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu caucgggacg cugcuggugu ggcucggccu gaacaccaag     2040

aacggcucca ucagccugac cugccucgcg cugggcggcg ugaugaucuu ccuguccacc     2100

gccgucagcg ccgacguggg gugcuccgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7815
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 3 GC

<400>  7815
gggagaaagc uuaccaugcg gaucaucaac gcccgcaagg agaagaagcg gcgcggcgcc       60

gacaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgaggucacc      120

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      180

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      420

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      480

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      540

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      600

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      660

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      720

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1140

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1200

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1320

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc     1380

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1440

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1620

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1680

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1800

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgucggcggg     1920

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     2040

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgcggugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7816
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 3 GC

<400>  7816
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc     1080

ggcuacgaga ccgacgagaa ccgcgccaag guggagguga cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac acggcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucucc gggcaccuca agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc gucgacaugc agacccugac gccggugggg     1680

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1800

acgcaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gaccgugcgc     1860

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7817
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 3 GC

<400>  7817
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucacccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcc      600

cccgcguaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu ggguggacgu cgugcuggag cacggcgggu gcgugaccgu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accacgaccg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggcgagg cguaccucga caagcagagc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag     1080

aaccgcgcca agguggaggu cacccccaac uccccgcggg ccgaggccac ccucggcggg     1140

uucggcagcc uggggcugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgcgggcgc cgacacgggg accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagaccgucg uggugcucgg cagccaggag     1380

ggggcggucc acaccgcccu ggccggcgcc cuggaggcgg agauggacgg ggccaagggc     1440

cgccucuucu ccggccaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggg     1500

gugagcuacu cccugugcac ggccgccuuc accuucacca aggugcccgc cgagacccuc     1560

cacggcaccg ucacggugga ggugcaguac gcggggaccg acggccccug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug accccggugg gccggcugau cacggccaac     1680

cccgucauca ccgagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacagcu acaucgugau cggcgugggg gacaagaaga ucacccacca cuggcaccgc     1800

uccggcagca cgaucgggaa ggccuucgag gcgaccgucc ggggcgccaa gcgcauggcc     1860

gugcugggcg acaccgccug ggacuucggg agcgugggcg gggucuucaa cucccucggc     1920

aaggggaucc accagaucuu cggcgccgcg uucaagagcc uguucggcgg gauguccugg     1980

uucagccaga uccugaucgg cacccugcuc guguggcugg ggcugaacac gaagaacggc     2040

uccaucagcc ugaccugccu ggcccucggg ggcgugauga ucuuccucuc caccgccguc     2100

agcgccgacg ugggcugcuc cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7818
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 3 GC

<400>  7818
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccgcaag      360

aagggcgagg cccggcgcag ccggcgcgcg gugacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccggug caucggggug uccaaccgcg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cggagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcggacccug      900

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc     1080

ggcuacgaga ccgacgagaa ccgggccaag guggagguga cccccaacuc cccgcgcgcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg gaccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac acggcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucucc gggcaccuca agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc gucgacaugc agacccugac gccggugggg     1680

cggcugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc gaccgugcgg     1860

ggcgccaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7819
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 3 GC

<400>  7819
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaccgu caacgacauc     1080

ggcuacgaga ccgacgagaa ccgcgccaag guggagguga cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac acggcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucucc gggcaccuca agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc gucgacaugc agacccugac gccggugggg     1680

cgccugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1800

acgcaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gaccgugcgc     1860

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7820
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 3 GC

<400>  7820
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacc     1080

gacgagaacc gcgccaaggu cgaggugacc cccaacuccc cgcgggccga ggcgacccuc     1140

gggggcuucg ggagccuggg ccuggacugc gagccccgca ccggccucga cuucuccgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcucgugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggggccgac acgggcaccc cgcacuggaa caacaaggag     1320

gcccuggucg aguucaagga cgcccacgcg aagcggcaga ccgugguggu ccucgggagc     1380

caggagggcg ccgugcacac cgcccuggcc ggggcgcugg aggccgagau ggacggcgcc     1440

aagggcaagc uguucuccgg gcaccucaag ugccgccuga agauggacaa gcuccggcug     1500

aagggcguga gcuacucccu gugcacggcc gccuucaccu ucaccaaggu ccccgcggag     1560

acccuccacg ggaccgugac gguggagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc cggucgggcg ccugaucacg     1680

gccaaccccg ugaucaccga gagcaccgag aacuccaaga ugaugcucga gcuggacccg     1740

cccuucggcg acagcuacau cgugaucggg gucggcgaca agaagaucac ccaccacugg     1800

caccgguccg ggagcacgau cggcaaggcc uucgaggcga ccgugcgcgg cgccaagcgg     1860

auggccgugc ugggggacac cgccugggac uucggcagcg ucgggggcgu guucaacucc     1920

cucgggaagg gcauccacca gaucuucggc gccgcguuca agagccuguu cgggggcaug     1980

uccugguuca gccagauccu gaucgggacc cugcucgugu ggcugggccu gaacacgaag     2040

aacgggucca ucagccugac cugccucgcc cugggcggcg ucaugaucuu ccucuccacc     2100

gccgugagcg ccgacguggg gugcuccguc gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7821
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 3 GC

<400>  7821
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggcg gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7822
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 3 GC

<400>  7822
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacgcgagcg uggggaucgu cggccugcuc cugaccaccg ccauggccgc cgaggugacg      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgcgggcga ggccaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac gaccagcacc uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcg gucacgcucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac cuggcuggag ucccgggagu acacgaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg ccgccgccgc gaucgccugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca cgguggacau cgagcugguc accaccaccg ugagcaacau ggcggaggug      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacg      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguucg cgugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggcgg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggccccugca aggugccggc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7823
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 3 GC

<400>  7823
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg cuggcgcugg ccgccgccgc caucgcgugg      540

cuccugggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcucguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccucgacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcuggggu cccaggaggg cgccguccac accgcgcugg ccggggcccu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7824
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 3 GC

<400>  7824
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu gguaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7825
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 3 GC

<400>  7825
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgagauc      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcucgggu cccaggaggg cgccgugcac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7826
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 3 GC

<400>  7826
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcuggc ccagauggcg gucgacaugc agacccugac cccggugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7827
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 3 GC

<400>  7827
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg acccuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagacgc gcagccagac cuggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgcgaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7828
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 3 GC

<400>  7828
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccguggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7829
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 3 GC

<400>  7829
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcgcc uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcg gucacgcucc ccagccacuc cacccggaag      420

cugcagaccc gcccccagac cuggcuggag agccgggagu acacgaagca ccucauccgc      480

guggagaacu ggaucuuccg gaacccgggg uucgcccugg ccgccgccgc gaucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca cgguggacau cgagcugguc accaccaccg uguccaacau ggcggaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacg      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg cgugcagcaa gaagaugacg ggcaagucca uccagccgga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacag cccccgggcc     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccgcu gcccuggcac gccggggcgg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggcccgugca aggugcccgc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgcggccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca cggccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7830
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 3 GC

<400>  7830
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag caagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7831
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 3 GC

<400>  7831
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccacccg ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccggug caucggggug uccaaccgcg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cggagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg cugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcggacccug      900

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgggcgaag gucgagauca cgccgaacag cccccgcgcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg gaccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg gcucuccagc gggcaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cgccugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccggag cggguccacg aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7832
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 3 GC

<400>  7832
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggagcgcc       60

gacaccuccg ugggcaucgu cgggcugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccggggca gcgccuacua cauguaccug gaccgcaacg acgccgggga ggcgaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acggcacgug ccaccacaag      360

aagggggagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggc uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucgggu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      660

ggcggcaccu gggucgacgu ggugcuggag cacgggggcu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggggagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcuggggcaa cgggugcggc cuguucggga agggcucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc gggaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg gcaugaucgu gaacgacacc     1080

gggcacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucggcggguu cggcucccug gggcuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggcgccg acacggggac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggca gccaggaggg ggccguccac accgcgcugg ccggcgcccu ggaggccgag     1440

auggacgggg ccaagggccg ccucuccagc ggccaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggggu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccgug acggucgagg ugcaguacgc cgggaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaccc agacccugac gcccgugggc     1680

cggcugauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg ggacuccuac aucgucaucg gcguggggga gaagaagauc     1800

acgcaccacu ggcaccgcag cggcagcacc aucgggaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggcgac accgccuggg acuucggguc cgugggcggg     1920

gcccucaaca gccugggcaa ggggauccac cagaucuucg gcgccgcguu caagucccug     1980

uucggcggga ugagcugguu cucccagauc cugaucggca cgcuccugau guggcugggg     2040

cucaacacca agaacggcag caucucccug augugccugg cccucggggg cgugcugauc     2100

uuccugagca ccgccgucuc cgccgacgug ggcugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7833
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 3 GC

<400>  7833
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccaacg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccggggga gcgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgcccgcc ggauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7834
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 3 GC

<400>  7834
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gagaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7835
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 3 GC

<400>  7835
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu cccugcgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcucguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccucgacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcuggggu cccaggaggg cgccguccac accgcgcugg ccggggcccu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7836
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 3 GC

<400>  7836
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccaacg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccggggga gcgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggauguuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7837
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 3 GC

<400>  7837
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggauccugcg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cuccugggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcucguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccucgacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcuggggu cccaggaggg cgccguccac accgcgcugg ccggggcccu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg ggaucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7838
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 3 GC

<400>  7838
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc agcaccaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc gacgaucucc gacaucgcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgccguc     1380

gugcucgggu cccaggaggg cgcggugcac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc gcagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgcgaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7839
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 3 GC

<400>  7839
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7840
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 3 GC

<400>  7840
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagc ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7841
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 3 GC

<400>  7841
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gagaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

aucgagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ugaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucgggguc agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcucgggu cccaggaggg cgccgugcac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7842
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 3 GC

<400>  7842
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaucg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7843
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 3 GC

<400>  7843
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ugaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccugac ccccgugggg     1680

cggcugauca cggcgaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagcuuc augugccugg cgcugggcgg cgugcucauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7844
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 3 GC

<400>  7844
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcucagcucc gggcaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cgccugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7845
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 3 GC

<400>  7845
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gaggcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7846
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 3 GC

<400>  7846
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcgccgac     1620

ggccccugca aggugccggc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7847
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 3 GC

<400>  7847
gggagaaagc uuaccaugcg caucaucaac gccggcaagg agaagaagcg gcgcggggcc       60

gacaccaacg ugggcaucgu cgggcugcuc cugaccacgg cgauggccgc cgaggugacc      120

cggcgcggca gcgccuacua cauguaccug gaccggaacg acgccgggga ggcgaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggggugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acggcacgug ccaccacaag      360

aagggggagg cccgccggag ccgccgggcc gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucauccgg      480

guggagaacu ggaucuuccg caaccccggc uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucgggu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccggug caucggcgug uccaaccgcg acuucgugga gggcaugagc      660

gggggcaccu gggucgacgu ggugcuggag cacgggggcu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cggagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg cugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcggacccug      900

guggaccgcg gguggggcaa cgggugcggc cuguucggga agggcucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccggauca ugcugagcgu ccacgggucc cagcacagcg gcaugaucgu gaacgacacc     1080

gggcacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgcgcc     1140

gaggccaccc ucggcggguu cggcucccug ggccuggacu gcgagccccg gaccgggcuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggcgccg acacggggac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug     1380

gugcucggca gccaggaggg ggccguccac accgcgcugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaaggggcg gcucuccagc ggccaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggggu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccgug acggucgagg ugcaguacgc cggcaccgac     1620

gggcccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggc     1680

cgccugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg ggacuccuac aucgucaucg gcguggggga gaagaagauc     1800

acccaccacu ggcaccggag cggcuccacg aucggcaagg ccuucgaggc caccgugcgc     1860

ggggcccggc gcauggcggu ccugggcgac accgccuggg acuucgggag cgugggcggg     1920

gcccucaaca gccugggcaa gggcauccac cagaucuucg gggccgcguu caagucccug     1980

uucggcggga ugagcugguu cucccagauc cugaucggca cccuccugau guggcugggg     2040

cucaacacga agaacggcag caucucccug augugccugg cccucggcgg ggugcugauc     2100

uuccugagca ccgccgucuc cgccgacgug ggcugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7848
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 3 GC

<400>  7848
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gagaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaagg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7849
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 3 GC

<400>  7849
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacaugguca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgucacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcgcacg ccaagcggca gaccguggug     1380

guccucgggu cccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccugac ccccgugggg     1680

cggcugauca cggcgaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cgcucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7850
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 3 GC

<400>  7850
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccaacg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccggggga gcgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacau gaccuccacc uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacgcucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac cuggcuggag agccgggagu acacgaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca cgguggacau cgagcugguc accaccaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggccu ccgacagccg gugccccacg      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca cggccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7851
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 3 GC

<400>  7851
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccgccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggcgg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggccccugca aggugccggc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagacc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7852
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 3 GC

<400>  7852
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gagaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

cuggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccga ccguggacau cgagcucguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgcccaacag cccgcgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccucgacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acacgggcac cccgcacugg     1320

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcuggggu cccaggaggg cgccguccac accgcgcugg ccggggcccu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcacgg cggccuucac cuucaccaag     1560

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcg gucgacaugc agacccugac cccggugggg     1680

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccucggggac accgccuggg acuucggcag cgucgggggc     1920

gcccugaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cucaucggga cccugcugau guggcucggc     2040

cugaacacga agaacggguc caucagccug augugccucg cccugggcgg cgugcugauc     2100

uuccucucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7853
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 3 GC

<400>  7853
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucgggauc agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggauccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacguc gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7854
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 3 GC

<400>  7854
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacacg      240

ugcgacgcca ccauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cauccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacgcca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7855
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 3 GC

<400>  7855
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccagcgguc ccgccgggcc gucacccucc ccagccacuc cacccgcaag      420

cugcagaccc ggagccagac guggcuggag ucccgcgagu acaccaagca ccucauccgg      480

guggagaacu ggaucuuccg caaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccggug caucggggug agcaaccgcg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg ugagcaacau ggccgaggug      780

cgguccuacu gcuacgaggc gagcaucucc gacauggcca gcgacagccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcggacccug      900

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccggauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgcgcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg gaccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug     1380

gugcucgggu cccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg gcucagcucc gggcaccuga agugccgccu gaagauggac     1500

aagcuccggc ugaagggcgu gagcuacucc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cgccugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc caccgugcgc     1860

ggcgccaagc ggauggcggu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7856
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 3 GC

<400>  7856
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcaggcgc gcagccagac cuggcuggag ucccgggagu acacgaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg ccgccgccgc gaucgccugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca cgguggacau cgagcugguc accaccaccg ugagcaacau ggcggaggug      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacg      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguucg cgugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcc     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggcgg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucgggu cccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggccgag     1440

auggacggcg cgaagggccg ccucagcucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1620

ggccccugca aggugccggc gcagauggcc gucgacaugc agacccugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgcgaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacca agaacggguc caucagccug augugccugg cccucggcgg cgugcugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7857
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 3 GC

<400>  7857
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggcgaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accaccacgg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgcgaag gucgagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcgcuggu ggaguucaag gacgcccacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcgcugg ccggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg cggccuucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcg gucgacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggcggu ccugggggac accgccuggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgcguu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacgggag caucucccug augugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7858
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 3 GC

<400>  7858
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga ccaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac accggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacgcucca cgggaccgug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc gucgacaugc agacgcugac ccccgugggg     1680

cggcugauca ccgccaaccc cgugaucacc gagggcacgg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg ggacagcuac aucgucaucg gcguggggga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcagcacc aucggcaagg cguucgaggc caccgugcgc     1860

ggggccaagc ggauggccgu ccugggcgac acggccuggg acuucgggag cgugggcggg     1920

gugcucaacu cccugggcaa gggcauccac cagaucuucg gggcggccuu caagagccug     1980

uucggcggga uguccugguu cagccagauc cugaucggca cccuccugau guggcugggg     2040

cucaacacca agaacggcuc caucagccug augugccugg cccucggcgg gguccugauc     2100

uuccugucca ccgccgugag cgcggacgug ggcugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7859
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 3 GC

<400>  7859
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcaacg acgccggcga ggcgaucagc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccagcacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcuc ccggcgcgcc gucacccucc ccagccacuc cacccggaag      420

cugcagaccc gcagccagac guggcuggag ucccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcgcugg ccgccgccgc caucgcgugg      540

cugcucggca gcuccaccag ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucacggc cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucagcaacau ggcggaggug      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugacgugc      960

gccaaguucg cgugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugcucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcc     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccug     1200

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggc ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gcgggggccg ccacgggcac gccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcggca gaccguggug     1380

guccuggggu cccaggaggg cgccgugcac accgcccugg ccggggcgcu cgaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuga agugccggcu caagauggac     1500

aagcugcgcc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agaccgucga cgggaccgug acgguggagg gccaguacgg cgggaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccugac ccccgugggg     1680

cggcugauca cggccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcagcacg aucgggaagg ccuucgaggc gaccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcgcucaacu cccugggcaa ggggauccac cagaucaucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggcuc caucagccug augugccugg cccucggggg cgugcugauc     2100

uuccugucca ccgccgugag cgggggcgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7860
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 3 GC

<400>  7860
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga ccaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgcguucac cuucuccaag     1560

auccccgccg agacccucca cgggaccgug acggaggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggcgaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

gcccucaacu cccuggggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccuccugau guggcugggc     2040

cucaacacga agaacggguc caucagccug augugccugg cgcucggcgg cgugcugauc     2100

uuccugucca ccgccgugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7861
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 3 GC

<400>  7861
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga ccaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgcgaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcag cgggagcacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu ccugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug augugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7862
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 3 GC

<400>  7862
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggu ggaggugacc      120

cgccggggga acgccuacua cauguaccug gaccgcuccg acgccggcga ggccaucagc      180

uuccccacca cgauggggau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccagcacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cgcggcgcuc ccggcgcgcc gugacccugc ccagccacuc cacccggaag      420

cuccagacgc gcagccagac cuggcuggag ucccgggagu acaccaagca ccugauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccucg ccgcggccgc caucgccugg      540

cugcugggca gcuccaccag ccagaagguc aucuaccucg ugaugauccu gcugaucgcc      600

ccggcguacu ccauccgcug caucggggug agcaaccggg acuucgucga gggcaugucc      660

ggcgggacgu ggguggacgu gguccucgag cacggcgggu gcgugaccgu gauggcccag      720

gacaagccca ccgucgacau cgagcuggug accacgaccg ugagcaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacagccg gugccccacc      840

cagggcgagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcuc      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccucgag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag guggagauca ccccgaacag cccccgggcc     1140

gaggcgaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccug     1200

gacuucagcg accucuacua ccugacgaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu cccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcccacg cgaagcggca gaccguggug     1380

guccuggggu cccaggaggg cgccgugcac accgcccucg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccugagcucc gggcaccuca agugccggcu gaagauggac     1500

aagcugcgcc ucaagggcgu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccugca cgggacgguc accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccucac gcccgugggg     1680

cggcugauca ccgccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1740

gagcucgacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg cguucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

gcgcugaacu cccucgggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu cuggcugggc     2040

cugaacacca agaacggguc caucagccuc augugccugg cccugggcgg cgugcucauc     2100

uuccugucca ccgcggugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7863
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 3 GC

<400>  7863
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggc cgaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga ccaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguggugu acgggaccug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gucacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga cggacgagaa ccgcgccaag gucgagauca ccccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc gucgacaugc agacccugac gcccgugggg     1680

cggcugauca ccgcgaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1800

acgcaccacu ggcaccgcag cgggagcacc aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu ccugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cgcuccugau guggcugggc     2040

cucaacacca agaacgggag caucucccug gugugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacgug gggugcagcg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7864
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 3 GC

<400>  7864
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcacc       60

gacaccagcg uggggaucgu cggccugcuc cugacgaccg ccauggcggu ggaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cgcucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      360

aagggcgagg cgcggcgcag ccggcgcgcc gugacccucc ccucccacag cacccggaag      420

cugcagacgc gcucccagac cuggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg ccgcggccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcc      600

ccggcguaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggacgu ggguggacgu gguccuggag cacggcgggu gcgugaccgu gauggcccag      720

gacaagcccg ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcgcacg ccaagcggca gaccguggug     1380

guccucggga gccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggucccggc ccagauggcc guggacaugc agacccugac ccccgugggg     1680

cggcugauca cggcgaaccc cgucaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggugggcga gaagaagauc     1800

acccaccacu ggcaccgcag cgggagcacg aucggcaagg ccuucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccuggu guggcugggc     2040

cucaacacga agaacgggag caucucccug accugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccguguc cgccgacguc gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7865
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 3 GC

<400>  7865
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgu ggaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cccucgggau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga cgaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc uggguggucu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgu caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcc      600

ccggcguaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggcgagg cguaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcc     1140

gaggcgaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgcgg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga gaagaagauc     1800

acccaccacu ggcaccgcag cgggagcacg aucggcaagg cguucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcuc cgucgggggc     1920

gcgcucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccuggu guggcugggc     2040

cucaacacga agaacgggag caucucccug accugccugg cccucggcgg cgugcugauc     2100

uuccugagca ccgcggucuc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7866
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 3 GC

<400>  7866
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgu ggaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcga ggccaucucc      180

uuccccacca cccucggggu caacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cgccgaacag cccccgggcg     1140

gaggccaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acacgggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg ccgcguucac cuucaccaag     1560

auccccgccg agacccucca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca cggcgaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1800

acccaccacu gguaccgcag cgggagcacg aucggcaagg ccuucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccuggu guggcugggc     2040

cucaacacga agaacgggag caucucccug accugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccgucuc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7867
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 3 GC

<400>  7867
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agaagaagcg ccggggcgcc       60

gacaccagcg uggggaucgu cggccugcuc cugaccacgg cgauggccgu ggaggugacc      120

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      180

uuccccacca cccucggggu caacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcga cgaugagcua cgagugcccg augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccggcgcag ccggcgcgcc gugacccucc ccucccacag cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg cggccgccgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

ccggccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagauca ccccgaacag cccccgggcg     1140

gaggccacgc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcgcacg ccaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuccagc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cuccuacagc cugugcaccg ccgcguucac guucaccaag     1560

auccccgccg agacccucca cgggaccgug accguggagg uccaguacgc cggcacggac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccugac ccccgucggg     1680

cggcugauca ccgcgaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1800

acccaccacu ggcaccgcag cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcuc cgucgggggc     1920

gcccucaaca gccuggggaa gggcauccac cagaucuucg gcgccgccuu caagucccug     1980

uucgggggca ugagcugguu cucccagauc cugaucggga cccuccuggu guggcugggc     2040

cucaacacca agaacgggag caucucccug acgugccugg cgcucggcgg cgugcugauc     2100

uuccugagca ccgccgucuc cgccgacgug gggugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7868
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 3 GC

<400>  7868
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucacccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcc      600

cccgcguaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggacgu ggguggacgu cgugcuggag cacggcgggu gcgugaccgu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accacgaccg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcca gcgacucccg gugccccacc      840

cagggcgagg cguaccucga caagcagagc gacacccagu acgugugcaa gcgcacgcug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacuc cccgcgggcc     1140

gaggccaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg cacgggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gacggucgug     1380

gugcucggga gccaggaggg cgcgguccac accgcccugg ccggggcccu ggaggcggag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacgcucca cgggaccguc accguggagg ugcaguacgc gggcaccgac     1620

ggccccugca agguccccgc ccagauggcc guggacaugc agacgcugac cccggugggg     1680

cggcugauca ccgccaaccc cgucaucacc gagagcacgg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc gaccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac acggccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgcguu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca cggccgucag cgccgacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7869
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 3 GC

<400>  7869
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca accucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac gaccuccacc ugggucgugu acgggaccug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacgcucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac cuggcuggag agccgcgagu acacgaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg ccgccguggc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccga cgguggacau cgagcugguc accaccaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggcca gcgacucccg gugccccacg      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguga cccccaacuc cccgcgggcg     1140

gaggccacgc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcgcacg ccaagcggca gaccguggug     1380

guccucggga gccaggaggg cgccgugcac accgcccugg cgggggcccu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgcguucac guucaccaag     1560

guccccgccg agacccucca cgggaccgug accguggagg uccaguacgc cggcacggac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1680

cggcugauca ccgcgaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcgugcac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcucgu cuggcugggc     2040

cugaacacca agaacggguc caucagccuc acgugccugg cccugggcgg cgugaugauc     2100

uuccucucca ccgcggugag cgccgacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7870
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 3 GC

<400>  7870
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg uggccguggc caucgccugg      540

cugcucggcu ccagcaccuc ccagaagguc aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgucga gggcaugagc      660

ggcgggaccu ggguggacgu gguccuggag cacggcgggu gcgugacggu gauggcccag      720

gacaagccga ccgucgacau cgagcuggug accaccacgg uguccaacau ggccgagguc      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgucacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagguga cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acacgggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgugguc     1380

gugcucggga gccaggaggg cgccgugcac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu cagcuacucc cugugcacgg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggaccgug acggucgagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1680

cggcugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg cguucgaggc caccgugcgg     1860

ggcgccaagc gcauggccgu ccugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcucgu guggcugggc     2040

cugaacacga agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgugag cgcggacguc gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7871
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 3 GC

<400>  7871
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca accucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac gaccuccacc ugggucgugu acgggaccug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gugacgcucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac cuggcuggag agccgcgagu acacgaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggg uucgcccugg ccgccguggc caucgcgugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ucaugauccu gcucaucgcc      600

cccgccuaca gcauccgcug caucggggug uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccga cgguggacau cgagcugguc accaccaccg uguccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc guccaucagc gacauggcca gcgacucccg gugccccacg      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgucugcaa gcgcacccug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugaccugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag     1080

aaccgcgcca aggucgaggu gacccccaac uccccgcggg cggaggccac ccucggcggg     1140

uucggcagcc uggggcugga cugcgagccc cgcacgggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccug     1320

gucgaguuca aggacgcgca cgccaagcgg cagacggugg ugguccucgg cagccaggag     1380

ggggccgugc acaccgcccu ggcgggcgcc cuggaggccg agauggacgg ggccaagggc     1440

cgccucuucu ccggccaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggg     1500

gugagcuacu cccugugcac cgccgcguuc accuucacga agguccccgc cgagacccuc     1560

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacgcug accccggucg gccggcugau caccgcgaac     1680

cccgugauca ccgagagcac ggagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacagcu acaucgugau cggcgucggg gacaagaaga ucacccacca cuggcaccgc     1800

uccggcagca ccaucgggaa ggccuucgag gccaccgugc ggggcgccaa gcgcauggcc     1860

gugcugggcg acacggcgug ggacuucggg agcgucggcg ggguguucaa cucccucggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg gauguccugg     1980

uucagccaga uccugaucgg cacccugcuc guguggcugg ggcugaacac caagaacggc     2040

uccaucagcc ugaccugccu ggcccucggg ggcgucauga ucuuccucuc cacggcggug     2100

agcgccgacg ugggcugcuc cgucgacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7872
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 3 GC

<400>  7872
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac acggcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgcgg agacccucca cgggacgguc accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc guggacaugc agacccugac gccggugggg     1680

cggcugauca ccgccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1800

acgcaccacu ggcaccgcuc cgggagcacc aucggcaagg cguucgaggc caccguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgcggccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cgcugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccug accugccucg cccugggcgg cgugaugauc     2100

uuccucucca ccgccgucag cgcggacgug gggugcuccg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7873
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 3 GC

<400>  7873
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccagcacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacuc cccgcgggcc     1140

gaggcgaccc ucgggggcuu cgggagccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucuccg accuguacua ccugaccaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggggccg acacgggcac cccgcacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcggca gaccgucgug     1380

gugcucggga gccaggaggg cgccguccac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucucc gggcaccuga agugccggcu gaagauggac     1500

aagcuccgcc ugaagggcgu gagcuacucc cugugcacgg ccgugugcac cgccgcgaag     1560

guccccgccg agacccucca cgggaccgug accguggagg uccaguacgc cggcacggac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1680

cggcugauca ccgcgaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcuc     1740

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc cacggugcgg     1860

ggcgccaagc gcauggccgu gcugggggac accgcguggg acuucggcag cgucgggggc     1920

guguucaacu cccucgggaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcucgu guggcugggc     2040

cugaacacca agaacggguc caucagccuc acgugccugg cccugggcgg cgucaugauc     2100

uuccucucca ccgcggugag cgccgacgug gggugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7874
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 3 GC

<400>  7874
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacgcgagca ucgggaucgu gucccugcuc cugaccaccg ucauggccgc cgagaucacg      120

cggcgcggca gcgccuacua cauguaccug gaccgguccg acgccgggaa ggcgaucagc      180

uucgugacca cccucggcgu gaacaagugc cacguccaga ucauggaccu ggggcacaug      240

ugcgacgcca ccauguccua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac gaccagcacc ugggucgugu acgggaccug ccaccacaag      360

aagggggagg cccgccgguc ccgccgggcc gugacgcucc ccagccacuc cacccgcaag      420

cugcagaccc ggagccagac cuggcuggag ucccgcgagu acacgaagca ccucaucaag      480

gucgagaacu ggaucuuccg gaaccccggc uucgcgcugg uggccguggc caucgccugg      540

cugcucggga gcuccaccag ccagaagguc aucuaccugg ugaugauccu gcucaucgcc      600

cccgcguacu ccauccgcug caucggcgug agcaaccggg acuucgucga ggggaugucc      660

ggcggcaccu ggguggacgu gguccuggag cacgggggcu gcgugaccgu gauggcccag      720

gacaagccga cggucgacau cgagcuggug accaccaccg ugagcaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cagcaucagc gacauggccu ccgacagccg gugccccacg      840

cagggggagg cguaccucga caagcagucc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcuggggcaa cgggugcggc cuguucggga agggcagccu cgugaccugc      960

gccaaguuca cgugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu gcacggcagc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca agguggaggu gacccccaac agcccgcggg ccgaggccac ccucgggggc     1140

uucggguccc ugggccugga cugcgagccc cgcacggggc ucgacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cugcccuggc acuccggcgc ggacaccgag accccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagcgg cagacggugg ucgugcucgg cagccaggag     1380

ggggccgugc acaccgcgcu ggccggcgcc cuggaggccg agauggacgg ggcgaagggc     1440

cgccucucca gcgggcaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggc     1500

gucuccuaca gccugugcac cgccgccuuc accuucacga aggugcccgc cgagacccuc     1560

cacggcaccg ugaccgucga ggugcaguac gccgggcggg acggccccug caaggugccc     1620

gcgcagaugg ccgucgacau gcagacccug acgccggugg ggcgccugau caccgccaac     1680

cccgugauca ccgaguccac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1740

ggcgacagcu acaucgucau cggcgugggg gacaagaaga ucacgcacca cuggcaccgg     1800

uccggcagca ucaucgggaa ggccuucgag gccaccgugc gcggcgcgaa gcggauggcc     1860

guccuggggg acaccgccug ggacuucggc uccgugggcg ggguguucaa cagccucggc     1920

aaggggaucc accagaucuu cggcgccgcc uucaaguccc uguucggggg caugagcugg     1980

uucucccaga uccugaucgg cacccugcuc gucuggcugg ggcugaacac gaagaacggc     2040

agcaucuccc ugaccugccu ggcgcucggg ggcgugauga ucuuccucag caccgccgug     2100

uccgccgacg ucgggugcag cguggacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7875
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 3 GC

<400>  7875
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucgu gggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu caacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc uggguggugu acgggacgug ccaccacaag      360

aagggcgagg cccgccggag ccgccgggcg gucacccucc ccucccacag cacccgcaag      420

cugcagaccc ggucccagac guggcuggag agccgcgagu acaccaagca ccucaucaag      480

guggagaacu ggaucuuccg gaaccccggg uucgcccugg uggccgucgc caucgccugg      540

cugcucggcu ccagcaccuc ccagaaggug aucuaccugg ugaugauccu gcucaucgcg      600

cccgccuaca gcauccgcug caucgggguc uccaaccggg acuucgugga gggcaugagc      660

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugacggu cauggcccag      720

gacaagccga ccguggacau cgagcuggug accaccacgg ucuccaacau ggccgaggug      780

cgcagcuacu gcuacgaggc cuccaucagc gacauggcga gcgacucccg gugccccacc      840

cagggcgagg ccuaccucga caagcagagc gacacccagu acgugugcaa gcgcacccug      900

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu cgugacgugc      960

gccaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag     1020

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag     1080

aaccgcgcca agguggaggu cacccccaac uccccgcggg ccgaggcgac ccucggcggg     1140

uucggcagcc uggggcugga cugcgagccc cgcaccggcc ucgacuucuc cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcuc gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggg acgccgcacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgcgaagcgg cagaccgucg uggugcucgg cagccaggag     1380

ggggccgucc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1440

cgccucuucu ccggccaccu gaagugccgg cugaagaugg acaagcuccg ccugaagggg     1500

gugagcuacu cccugugcac cgccgccuuc acguucacca aggugcccgc ggagacccuc     1560

cacggcaccg ucaccgugga ggugcaguac gccgggacgg acggccccug caaggucccc     1620

gcccagaugg ccguggacau gcagacccug accccggugg gccggcugau caccgccaac     1680

cccgucauca cggagagcac cgagaacucc aagaugaugc ucgagcugga cccgcccuuc     1740

ggggacagcu acaucgugau cggcgugggg gacaagaaga ucacccacca cuggcaccgc     1800

uccggcagca ccaucgggaa ggcguucgag gccacggucc ggggcgccaa gcgcauggcc     1860

gugcugggcg acaccgccug ggacuucggg agcgugggcg gggucuucaa cucccucggc     1920

aaggggaucc accagaucuu cggcgcggcc uucaagagcc uguucggcgg gauguccugg     1980

uucagccaga uccugaucgg cacccugcuc guguggcugg ggcugaacac caagaacggc     2040

uccaucagcc ucacgugccu ggcccucggg ggcgugauga ucuuccuguc caccgccguc     2100

agcgcggacg ugggcugcuc cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  7876
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 3 GC

<400>  7876
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca cccucggggu gaacaagugc cacguccaga ucauggaccu gggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gccggacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgaga cccgccggag ccgccggucc gugagccucc gcuaccacua cacccggaag      420

cugcagaccc gcucccagac guggcuggag agccgggagu acaagaagca ccucaucaug      480

gucgagaacu ggaucuuccg caaccccggg uucgccaucg uguccguggc gaucaccugg      540

cugaugggca gccugaccuc ccagaagguc aucuaccucg ugaugaucgu gcugaucguc      600

cccgccuaca gcaucuccug caucggggug agcaaccggg accuggugga gggcaugucc      660

ggcgggaccu gggucgacgu ggugcucgag cacggcgggu gcgucacgga gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaugaccg ucagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc cagccugagc gacauggcgu ccgccagccg gugcccgacg      840

cagggcgagc ccucccucga caagcagagc gacacccagu ccgugugcaa gcgcacccug      900

ggcgaccggg gguggggcaa cgggugcggc aucuucggga agggcagccu ggugaccugc      960

uccaaguuca cgugcugcaa gaagaugccc ggcaagagca uccagcccga gaaccucgag     1020

uaccgcauca ugcugccggu ccacgggucc cagcacagcg gcaugaucgu caacgacauc     1080

gggcacgaga ccgacgagaa ccgcgccaag guggagguga cccccaacag cccccgggcc     1140

gaggccaccc ugggcggguu cggcucccuc ggccuggacu gcgagccccg cacggggcug     1200

gacuucuccg accucuacua ccugaccaug aacaacaagc acuggcuggu ccacaaggag     1260

ugguuccacg acaucccgcu ccccuggcac gcgggcgccg acaccgggac gccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcggca gaccgugguc     1380

gugcugggca gccaggaggg ggcggugcac accgcccucg ccggcgcccu ggaggccgag     1440

auggacggcg cgaaggggcg ccuguucucc ggccaccuca agugccggcu gaagauggac     1500

aagcugcgcc ucaagggggu cagcuacucc cugugcaccg ccgccuucac cuucacgaag     1560

gugcccgccg agacccugca cggcaccgug accgucgagg ugcagagcgc gggcaccgac     1620

gggccgugca aggugcccgc ccagauggcc gucgacaugc agacgcucac ccccgugggc     1680

cggcugauca ccgccaaccc cgugaucacc gaguccacgg agaacagcaa gaugaugcug     1740

gagcucgacc cgcccuucgg ggacagcuac aucgucaucg gcguggggga caagaagauc     1800

acccaccacu ggcaccgcuc cggcagcacc aucggcaagg ccuucgaggc gaccgugcgc     1860

ggggccaagc ggauggccgu ccugggcgac acggccuggg acuucggguc cgugggcggg     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gggccgcguu caagucccuc     1980

uucggcggga ugagcugguu cucccagauc cugaucggca cccugcuggu cuggcucggg     2040

cugaacacca agaacggcag caucucccug accugccucg cccugggcgg ggugaugauc     2100

uuccugagca cggccguguc cgccgacguc ggcugcagcg uggacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7877
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 3 GC

<400>  7877
gggagaaagc uuaccaugcg caucaucaac gcccggaagg agcgcaagcg gcgcggcgcc       60

gacaccagca ucgggaucau cggccugcuc cugaccacgg cgauggccgc cgagaucacc      120

cggcgcgggu ccgccuacua cauguaccug gaccggagcg acgccggcaa ggcgaucucc      180

uucgccacca ccaugggggu gaacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca cgaugagcua cgagugcccc augcuggacg agggcgugga gccggacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggacgug ccaccacaag      360

aagggcgagg cccgccccag ccggcgcgcg gugacccugc ccucccacag cacccggaag      420

cuccagaccc gcucccagac guggcuggag agccgggagu acaccaagca ccugaucaag      480

gucgagaacu ggauccuccg caaccccggg uucaccgugg ccgcccuggc cauggccugg      540

cugcucggca acuccaccag ccagcgggug aucuaccugg ucaugauccu gcucaucgcg      600

ccggccuacu ccauccgcug caucggggug agcaaccggg acuucgugga gggcaugucc      660

ggcgggacgu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcccag      720

gacaagccca ccguggacau cgagcugguc accacgaccg ugagcaacau ggccgaggug      780

cgcuccuacu gcuacgaggc cagcaucucc gacauggcga gcgacagccg gugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacgcug      900

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu cgugaccugc      960

gccaaguuca cgugcuccaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uaccgcauca ugcuguccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguga ccccgaacag cccccgggcc     1140

gaggcgaccc ucgggggcuu cgggucccug ggccuggacu gcgagccccg caccggccuc     1200

gacuucagcg accuguacua ccugacgaug aacaacaagc acuggcucgu gcacaaggag     1260

ugguuccacg acaucccccu gccguggcac gccggggccg acaccggcac gccccacugg     1320

aacaacaagg aggcccuggu cgaguucaag gacgcccacg cgaagcggca gaccguggug     1380

guccucgggu cccaggaggg cgccgugcac accgcccugg ccggggcgcu ggaggccgag     1440

auggacggcg ccaagggccg ccucuucagc gggcaccuga agugccggau caagauggac     1500

aagcugcgcc ucaagggcgu guccuacagc cugugcaccg ccgccuucac cuucuccaag     1560

guccccgcgg agacccugca cgggaccgug acgguggagg uccaguacgc cggccgggac     1620

ggccccugca aggugccggc ccagauggcc guggacaugc agacccucac ccccgucggg     1680

cgccugauca ccgccaaccc caucaucacg gagagcaccg agaacucccg gaugaugcug     1740

gagcucgacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1800

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg cguucgaggc cacgguccgg     1860

ggcgccaagc gcauggccgu gcugggggac accgccuggg acuucggcag cgucgggggc     1920

guguucaacu cccuggggaa gggcgugcac cagguguucg gcgcggccuu caagagccuc     1980

uucgggggca uguccugguu cagccagauc cugaucggga cccugcuggu cuggcucggc     2040

cugaacacca agaacggguc caucagccug gccugccucg cccugggcgg cgcgaugauc     2100

uuccugucca cggccgugag cgccgacgug gggugcuccg ucgacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  7878
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 3 GC

<400>  7878
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7879
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 3 GC

<400>  7879
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7880
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 3 GC

<400>  7880
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccgaga ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguu cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcga agcggauggc cgugcucggg gacaccgccu gggacuucgg cagcgugggc     1860

ggcgucuuca acucccuggg gaaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg ggacgcugcu gguguggcuc     1980

ggccugaaca ccaagaacgg cuccaucagc cugaccugcc ucgcgcuggg cggcgugaug     2040

aucuuccugu ccaccgccgu cagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7881
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 3 GC

<400>  7881
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccgagg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7882
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 3 GC

<400>  7882
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcgugcacg gcucccagca cagcggcaug     1020

aucgucaacg acaccgggua cgagaccgac gagaaccgcg ccaaggugga ggugaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacc     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggucgugcu cggcagccag gagggggccg ugcacacggc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggcaagcugu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaagauc cccgugcaga uggcggucga caugcagacc     1620

cugacgccgg ugggccggcu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggacaaga agaucacgca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg ugcggggcgc caagcgcaug gcgguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcug     1980

cucgucuggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccucgcccuc     2040

gggggcguga ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7883
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 3 GC

<400>  7883
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc ggaagaaggg ggaggcccgc cggagccgcc gggccgugac ccuccccucc      360

cacagcaccc gcaagcugca gacccggucc cagacguggc uggagagccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccggaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcgugcacg gcucccagca cagcggcaug     1020

aucgucaacg acaccgggua cgagaccgac gagaaccggg ccaaggugga ggugaccccc     1080

aacuccccgc gcgccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacc     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggccg ugcacacggc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggcaagcugu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaagauc cccgugcaga uggcggucga caugcagacc     1620

cugacgccgg ugggccgccu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggacaaga agaucacgca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gcgguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcug     1980

cucgucuggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccucgcccuc     2040

gggggcguga ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7884
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 3 GC

<400>  7884
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcgugcacg gcucccagca cagcggcaug     1020

accgucaacg acaucgggua cgagaccgac gagaaccgcg ccaaggugga ggugaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacc     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggucgugcu cggcagccag gagggggccg ugcacacggc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggcaagcugu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaagauc cccgugcaga uggcggucga caugcagacc     1620

cugacgccgg ugggccggcu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggacaaga agaucacgca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg ugcggggcgc caagcgcaug gcgguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcug     1980

cucgucuggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccucgcccuc     2040

gggggcguga ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7885
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 3 GC

<400>  7885
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucac ccugguggcc      480

gucgcgaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gccgaccgug gacaucgagc uggugaccac gaccgucucc      720

aacauggcgg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cgcuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acgagaaccg cgcgaaggug gaggucaccc ccaacucccc gcgggccgag     1080

gccacccucg ggggcuucgg gagccugggc cuggacugcg agccccgcac cgggcucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca cgggcacccc gcacuggaac     1260

aacaaggagg cgcuggugga guucaaggac gcccacgcca agcggcagac cgucguggug     1320

cucgggagcc aggagggcgc cguccacacc gcccuggcgg gggcccugga ggccgagaug     1380

gacggcgcca aggggcgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggcgugag cuacucccug ugcacggcgg ccuucaccuu caccaaggug     1500

cccgccgaga cccuccacgg gaccgucacg guggaggugc aguacgccgg caccgacggg     1560

cccugcaagg uccccgccca gauggcggug gacaugcaga cccugacccc ggugggccgc     1620

cugaucacgg ccaaccccgu caucaccgag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucggcga cagcuacauc gugaucgggg ugggcgacaa gaagaucacc     1740

caccacuggc accgguccgg gagcacgauc ggcaaggccu ucgaggccac cguccgcggg     1800

gccaagcgga uggcggugcu gggcgacacc gccugggacu ucggcagcgu cgggggcgug     1860

uucaacuccc ucgggaaggg cauccaccag aucuucgggg ccgccuucaa gagccuguuc     1920

ggcggcaugu ccugguucag ccagauccug aucgggaccc ugcucgugug gcugggccug     1980

aacacgaaga acggguccau cagccugacc ugccucgccc ugggcggggu gaugaucuuc     2040

cucuccaccg cggucagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  7886
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 3 GC

<400>  7886
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcgugcacg gcucccagca cagcggcaug     1020

aucggguacg agaccgacga gaaccgcgcc aaggucgagg ugacccccaa cuccccgcgg     1080

gccgaggcca cccucggcgg guucggcagc cuggggcugg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccugua cuaccugacc augaacaaca agcacuggcu cgugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg cggacacggg gaccccgcac     1260

uggaacaaca aggaggcccu ggucgaguuc aaggacgccc acgccaagcg gcagaccgug     1320

gugguccucg gcagccagga gggggccgug cacaccgcgc uggccggcgc ccuggaggcc     1380

gagauggacg gggcgaaggg caagcuguuc uccggccacc ugaagugccg ccugaagaug     1440

gacaagcucc gccugaaggg ggugagcuac ucccugugca cggccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gugacggugg agguccagua cgccgggacc     1560

gacggccccu gcaagauccc cgugcagaug gcgguggaca ugcagacccu gaccccgguc     1620

ggccggcuga ucacggccaa ccccgugauc accgagagca ccgagaacuc caagaugaug     1680

cucgagcugg acccgcccuu cggggacagc uacaucguga ucggcgucgg ggacaagaag     1740

aucacccacc acuggcaccg cuccggcagc acgaucggga aggccuucga ggccaccgug     1800

cggggcgcca agcgcauggc ggugcugggc gacaccgccu gggacuucgg gagcgucggc     1860

gggguguuca acucccucgg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg ggauguccug guucagccag auccugaucg gcacccugcu cguguggcug     1980

gggcugaaca cgaagaacgg cuccaucagc cugaccugcc ucgcccucgg gggcgucaug     2040

aucuuccucu ccaccgcggu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7887
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 3 GC

<400>  7887
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggccggggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7888
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 3 GC

<400>  7888
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggccuggc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggucaug      540

auccuccuga ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc ucgucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccucuu cgggaagggc      900

agccugguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcuc uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cucguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu gggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcucgagg ccgagaugga cggggccaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cugcacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7889
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 3 GC

<400>  7889
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacugguac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7890
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 3 GC

<400>  7890
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga cacccagacc     1620

cugacgcccg ugggccggcu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cgcuccggca gcaccaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7891
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 3 GC

<400>  7891
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg agauccgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg uggucgugcu cggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7892
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 3 GC

<400>  7892
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug cuggcccaga uggccgucga caugcagacc     1620

cugaccccgg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7893
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 3 GC

<400>  7893
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgacccu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcacgugggu gguguacggg      300

accugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacgcgcagc cagaccuggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg cacguggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga ccugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg gcagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagacggac gagaaccggg ccaaggucga gaucaccccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacgcccg ugggccggcu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cgcuccggca gcaccaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7894
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 3 GC

<400>  7894
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccguc gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7895
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 3 GC

<400>  7895
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcgcgugggu gguguacggg      300

accugccacc acaagaaggg cgaggcccgg cgcucccggc gcgccgucac gcuccccagc      360

cacuccaccc ggaagcugca gacccgcccc cagaccuggc uggagagccg ggaguacacg      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc cgggcuucgc ccuggcggcc      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgcgcccgc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gcccacggug gacaucgagc uggucaccac caccgugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggcguccgac      780

agccggugcc ccacgcaggg ggaggccuac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucgccugc agcaagaaga ugacggggaa guccauccag      960

ccggagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucaccccc     1080

aacagccccc gcgcggaggc cacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcg     1500

uucacguuca ccaagauccc cgccgagacc cuccacggca ccgugaccgu cgaggugcag     1560

uacgccggga cggacggccc gugcaaggug cccgcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcg aaccccguga ucacggaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggcu ccaccaucgg gaaggccuuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc gugggacuuc     1860

gggagcgugg gcggggcccu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcagcaucu cccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu gagcacggcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7896
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 3 GC

<400>  7896
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagcaaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7897
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 3 GC

<400>  7897
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc acccgccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccgcugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccgcg ccaaggucga gaucacgccg     1080

aacagccccc gggccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcucu ccagcggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccgccu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccacgaucgg gaaggcguuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcagcaucu cccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7898
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 3 GC

<400>  7898
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcacccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacgcccg ugggccggcu gaucaccgcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cgcagcggcu ccaccaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacgcuc     1980

cugauguggc uggggcucaa caccaagaac ggcagcaucu cccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7899
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 3 GC

<400>  7899
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacucccug cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc ucgucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccucuu cgggaagggc      900

agccugguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcuc uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cucguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu gggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcucgagg ccgagaugga cggggccaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cugcacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7900
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 3 GC

<400>  7900
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcaucga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggugaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac cacggucagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg uggucgugcu cggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7901
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 3 GC

<400>  7901
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc cugcggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggucaug      540

auccuccuga ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc ucgucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccucuu cgggaagggc      900

agccugguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcuc uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cucguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu gggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcucgagg ccgagaugga cggggccaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cugcacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcauc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcucg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7902
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 3 GC

<400>  7902
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucagcac caccgugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccacga ucuccgacau cgccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ccgucgugcu cggcucccag gagggggccg ugcacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucagcu acucccugug caccgccgcg     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcg aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggccuuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc gugggacuuc     1860

gggagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7903
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 3 GC

<400>  7903
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgagcugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcucg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7904
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 3 GC

<400>  7904
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caucgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggcguuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccacggcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7905
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 3 GC

<400>  7905
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggugaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucacggu ggaggugcag     1560

uacgcgggga ccgacggccc cugcaagguc ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggccuuc     1800

gaggcgaccg uccggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gcuucaugug ccuggcccuc     2040

gggggcgucc ugaucuuccu guccaccgcc gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7906
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 3 GC

<400>  7906
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggcaagcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccgccu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgguccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7907
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 3 GC

<400>  7907
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caaggacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7908
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 3 GC

<400>  7908
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggaggc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7909
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 3 GC

<400>  7909
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc ggcccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcg     1500

uucacguuca ccaagauccc cgccgagacc cuccacggca ccgugaccgu cgaggugcag     1560

uacgccggga cggacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcg aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc gugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agacccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7910
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 3 GC

<400>  7910
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccgggg ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcg aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc gugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccacggcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7911
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 3 GC

<400>  7911
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgcccuggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gccgaccgug gacaucgagc ucgucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccucuu cgggaagggc      900

agccugguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcuc uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccc     1080

aacagcccgc gcgccgaggc gacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacg     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu gggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcucgagg ccgagaugga cggggccaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug cacggccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cugcacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug cccgcccaga uggccgucga caugcagacc     1620

cugaccccgg ugggccggcu caucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu gaacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cacgaagaac ggcuccauca gccugaugug ccucgcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7912
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 3 GC

<400>  7912
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggucagcgac      780

agccggugcc ccacccaggg ggaggccuac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguca cgugcgcgaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc cacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgcggg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cuggucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggugguccu cggcucccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucacggu ggaggugcag     1560

uacgcgggga ccgacggccc cugcaagguc ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggccuuc     1800

gaggcgaccg uccggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgucc ugaucuuccu guccaccgcc gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7913
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 3 GC

<400>  7913
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcaucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac cacggucagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggca uccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucuccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggcu ccacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcagcaucu cccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7914
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 3 GC

<400>  7914
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgccaccaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcacgugggu gguguacggg      300

accugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaucc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg gcagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cgccaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccacggcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7915
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 3 GC

<400>  7915
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcag cggucccgcc gggccgucac ccuccccagc      360

cacuccaccc gcaagcugca gacccggagc cagacguggc uggagucccg cgaguacacc      420

aagcaccuca uccgggugga gaacuggauc uuccgcaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cggugcaucg gcgugagcaa ccgcgacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgguc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccgcugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg gaucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccgcg ccaaggucga gaucacgccg     1080

aacagccccc gggccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cggcagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccgccu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgguccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7916
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 3 GC

<400>  7916
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca ggcccgcagc cagaccuggc uggagucccg ggaguacacg      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggcggcc      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgcgccggc cuacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gcccacggug gacaucgagc uggucaccac caccgugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggcgagcgac      780

agccggugcc ccacgcaggg ggaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga ccugcgccaa guucgccugc uccaagaaga ugacgggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucaccccg     1080

aacagccccc gcgcggaggc cacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcg     1500

uucacguuca ccaagauccc cgccgagacc cuccacggca ccgugaccgu cgaggugcag     1560

uacgccggga cggacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcg aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggccuuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc gugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcgcuc     2040

gggggcgugc ugaucuuccu guccacggcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7917
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 3 GC

<400>  7917
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuucu ccaagauccc cgcggagacc cuccacggca ccgugaccga ggagguccag     1560

uacgccggga cggacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucaccgcc aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggcu ccaccaucgg gaaggcguuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccacggcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7918
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 3 GC

<400>  7918
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacacggg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggcgg uccacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca cggugaccgu cgaggugcag     1560

uacgcgggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacgcccg ugggccggcu gaucaccgcc aaccccguga ucaccgaggg gaccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggcgaca gcuacaucgu caucggggug     1740

ggcgagaaga agaucacgca ccacuggcac cgcuccggga gcaccaucgg caaggccuuc     1800

gaggcgaccg ugcggggcgc caagcgcaug gccguccugg gggacaccgc cugggacuuc     1860

ggcagcgucg ggggcgugcu caacucccug gggaagggca uccaccagau cuucggcgcc     1920

gcguucaaga gccuguucgg gggcaugucc ugguucagcc agauccugau cgggacgcuc     1980

cugauguggc ugggccucaa caccaagaac ggguccauca gccugaugug ccuggcccuc     2040

ggcggcgugc ugaucuuccu guccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7919
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 3 GC

<400>  7919
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

ggguccgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcagcaucu cccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7920
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 3 GC

<400>  7920
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcaacgcg uacuacaugu accuggaccg cuccgacgcc      120

ggggaggcca ucagcuuccc caccaccaug ggcaugaaca agugcuacau ccagaucaug      180

gaccucgggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu ggucuacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcucccggc gcgcggugac ccugcccagc      360

cacuccaccc ggaagcucca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuga uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccucgccgcc      480

gcggccaucg ccuggcugcu ggggagcucc accagccaga aggucaucua ccucgugaug      540

auccugcuga ucgccccggc cuacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

gucgagggga uguccggcgg caccugggug gacguggucc ucgagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gcccaccguc gacaucgagc uggugaccac cacggugagc      720

aacauggccg agguccgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggccuac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccucgugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccugguca cgugcgcgaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc ucgaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc cacccugggc ggguucggcu cccucgggcu ggacugcgag     1140

ccccggaccg gccuggacuu cagcgaccuc uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccucccgu ggcacgcggg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cuggucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggcgg ugcacaccgc ccucgccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccuga gcuccggcca ccucaagugc     1440

cggcugaaga uggacaagcu gcgccucaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgucacggu ggaggugcag     1560

uacgcgggga ccgacggccc cugcaagguc ccggcccaga uggccgugga caugcagacc     1620

cucacccccg ugggccggcu gaucacggcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcuggagcu cgacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggccuuc     1800

gaggcgaccg uccggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcccu gaacucccuc ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucgucuggc uggggcugaa cacgaagaac ggcuccauca gccucaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcc gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7921
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 3 GC

<400>  7921
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

ggguccgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcagcaucu cccuggugug ccuggcccuc     2040

gggggcgucc ugaucuuccu gagcaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7922
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 3 GC

<400>  7922
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacagcauc cgcugcaucg gcgugaacaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccuggugug ccuggcccuc     2040

gggggcgucc ugaucuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7923
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 3 GC

<400>  7923
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcuccgcg uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu ggucuacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgcggugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcc      480

gcggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gcccgccguc gacaucgagc uggugaccac caccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacgcaggg ggaggcguac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucgccugc agcaagaaga ugacggggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucaccccg     1080

aacagccccc gcgccgaggc gacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cuggucgagu ucaaggacgc ccacgcgaag     1320

cgccagaccg uggugguccu cggcagccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag gggguguccu acagccugug caccgccgcc     1500

uucacguuca ccaagauccc cgcggagacc cuccacggca ccgucaccgu ggaggugcag     1560

uacgccggga cggacggccc cugcaagguc ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcc aaccccguca ucacggaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggca gcaccaucgg gaaggcguuc     1800

gaggccacgg uccggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggguccgugg gcggggcgcu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cuggucuggc uggggcucaa caccaagaac ggcagcaucu cccugacgug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7924
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 3 GC

<400>  7924
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcuccgcg uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu ggucuacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgcggugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcc      480

gcggucaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgccccggc cuacagcauc cgcugcaucg gcgucuccaa ccgggacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggcgg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgccaa guucgcgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc cacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgcggacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggccg ugcacaccgc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucuccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgugacggu ggagguccag     1560

uacgccggga ccgacggccc cugcaaggug ccggcgcaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cgcagcggca gcacgaucgg gaaggccuuc     1800

gaggccaccg ugcggggcgc gaagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggguccgucg gcggggcccu caacagccug ggcaagggga uccaccagau cuucggcgcc     1920

gcguucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugguguggc uggggcucaa cacgaagaac ggcagcaucu cccugaccug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7925
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 3 GC

<400>  7925
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcuccgcg uacuacaugu accuggaccg cagcgacgcc      120

ggggaggcca ucuccuuccc caccacccuc ggcgucaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgcggugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcc      480

gcggccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgccccggc cuacagcauc cgcugcaucg gcgucuccaa ccgggacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gcccaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgccgaggc cacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgcggg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucuccu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgugacggu ggagguccag     1560

uacgcgggga ccgacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucacggcc aaccccguga ucaccgaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcguc     1740

ggggacaaga agaucaccca ccacugguac cgcagcggca gcacgaucgg gaaggccuuc     1800

gaggcgaccg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggguccgucg gcggggcccu caacagccug ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugguguggc uggggcucaa cacgaagaac ggcagcaucu cccugaccug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcc gucuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7926
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 3 GC

<400>  7926
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccguggagg ugacccgccg gggcuccgcg uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuuccc caccacccuc ggcgucaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug agcuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgcggugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcc      480

gcggccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgccccggc cuacagcauc cgcugcaucg gcgucuccaa ccgggacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gcccaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucgccugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg caucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaucgggca cgagaccgac gagaaccggg ccaaggugga gaucaccccg     1080

aacagccccc gcgccgaggc cacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugccgu ggcacgcggg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggucgugcu cggcagccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccucu ccagcggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggucuccu acagccugug caccgccgcc     1500

uucacguuca ccaagauccc cgccgagacc cuccacggca ccgugaccgu ggagguccag     1560

uacgcgggga cggacggccc cugcaaggug ccggcccaga uggccgugga caugcagacc     1620

cugacccccg ucggccggcu gaucaccgcc aaccccguga ucacggaguc caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggggacu ccuacaucgu gaucggcguc     1740

ggggacaaga agaucaccca ccacuggcac cgcagcggca gcaccaucgg gaaggccuuc     1800

gaggcgacgg ugcggggcgc caagcgcaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggguccgucg gcggggcccu caacagccug ggcaagggga uccaccagau cuucggcgcg     1920

gccuucaagu cccuguucgg cgggaugagc ugguucuccc agauccugau cggcacccuc     1980

cugguguggc uggggcucaa caccaagaac ggcagcaucu cccugacgug ccuggcccuc     2040

gggggcgugc ugaucuuccu gagcaccgcc gucuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7927
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 3 GC

<400>  7927
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucac ccugguggcc      480

gucgcgaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gccgaccgug gacaucgagc uggugaccac gaccgucucc      720

aacauggcgg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cgcuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccgcg cgaaggugga ggucaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcacgg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcg cugguggagu ucaaggacgc ccacgccaag     1320

cggcagacgg ucguggugcu cggcagccag gagggggccg uccacaccgc ccuggcgggc     1380

gcccuggagg ccgagaugga cggggccaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgcggcc     1500

uucaccuuca ccaaggugcc cgccgagacg cuccacggca ccgucaccgu ggaggugcag     1560

uacgccggga ccgacggccc cugcaagguc cccgcccaga uggcggugga caugcagacg     1620

cugaccccgg ugggccgccu gaucaccgcc aaccccguca ucaccgagag cacggagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggacaaga agaucaccca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gcggugcugg gcgacacggc cugggacuuc     1860

gggagcgugg gcggggucuu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucguguggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccuggcccuc     2040

gggggcguga ugaucuuccu cuccacggcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7928
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 3 GC

<400>  7928
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccaaccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacacgaccu ccaccugggu cguguacggg      300

accugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac gcuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagaccuggc uggagagccg ggaguacacg      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccuggcggcc      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgcccccgc guacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gccgacggug gacaucgagc uggucaccac caccgugucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacgcaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaucgggca cgagaccgac gagaaccgcg ccaaggucga ggugaccccc     1080

aacuccccgc gggccgaggc cacgcucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugcccu ggcacgcggg cgccgacacc     1260

gggacgccgc acuggaacaa caaggaggcc cuggucgagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggugguccu cggcagccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccuggagg cggagaugga cggggccaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucacguuca ccaagguccc cgccgagacc cuccacggca ccgugaccgu ggagguccag     1560

uacgcgggga cggacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugaccccgg ucggccgccu gaucaccgcc aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggacaaga agaucaccca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggcgacgg ugcgcggcgc caagcggaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcgggguguu caacucccuc ggcaaggggg ugcaccagau cuucggcgcc     1920

gcguucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucgucuggc uggggcugaa caccaagaac ggcuccauca gccucacgug ccuggcccug     2040

ggcggcguga ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7929
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 3 GC

<400>  7929
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgugac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggucaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

gucgagggga ugagcggcgg caccugggug gacguggucc uggagcacgg gggcugcgug      660

acggugaugg cgcaggacaa gccgaccguc gacaucgagc uggugaccac cacggugucc      720

aacauggccg agguccggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcgugcacg gcucccagca cagcggcaug     1020

aucgucaacg acaucgggca cgagaccgac gagaaccgcg ccaaggugga ggugaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacg     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggucgugcu cggcagccag gagggggccg ugcacaccgc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug cacggccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug cccgcgcaga uggccgucga caugcagacc     1620

cugaccccgg ugggccgccu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggacaaga agaucaccca ccacuggcac cgguccggca gcacgaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc gaagcggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucgucuggc uggggcugaa cacgaagaac ggcuccauca gccugaccug ccuggcgcuc     2040

gggggcguga ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7930
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 3 GC

<400>  7930
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccaaccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacacgaccu ccaccugggu cguguacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac gcuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagaccuggc uggagagccg ggaguacacg      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccuggcggcc      480

guggccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggucaug      540

auccugcuca ucgcccccgc guacagcauc cggugcaucg gcguguccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gccgacggug gacaucgagc uggucaccac caccgugucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacgcaggg ggaggcguac cucgacaagc agagcgacac ccaguacguc      840

ugcaagcgga cccuggugga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga ccugcgccaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acgagaaccg cgccaagguc gaggugaccc ccaacucccc gcgggccgag     1080

gccacccucg ggggcuucgg gagccugggc cuggacugcg agccccgcac ggggcucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcg ggcgccgaca ccggcacccc gcacuggaac     1260

aacaaggagg cccuggucga guucaaggac gcccacgcca agcggcagac gguggugguc     1320

cucgggagcc aggagggcgc ggugcacacc gcccuggccg gggcccugga ggcggagaug     1380

gacggcgcca aggggcgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggcgugag cuacucccug ugcaccgccg ccuucaccuu cacgaagguc     1500

cccgccgaga cccuccacgg gaccgugacc guggaggucc aguacgcggg caccgacggg     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cgcugacccc ggucggccgc     1620

cugaucaccg ccaaccccgu gaucaccgag agcacggaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucggcga cagcuacauc gugaucgggg ucggcgacaa gaagaucacc     1740

caccacuggc accgguccgg gagcaccauc ggcaaggccu ucgaggcgac cgugcgcggg     1800

gccaagcgga uggccgugcu gggcgacacg gccugggacu ucggcagcgu cgggggcgug     1860

uucaacuccc ucgggaaggg cauccaccag aucuucgggg ccgcguucaa gagccuguuc     1920

ggcggcaugu ccugguucag ccagauccug aucgggaccc ugcucgugug gcugggccug     1980

aacaccaaga acggguccau cagccugacc ugccucgccc ugggcggggu caugaucuuc     2040

cucuccacgg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  7931
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 3 GC

<400>  7931
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

gucgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gccgaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccgcg ccaaggugga ggucaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga cgaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacc     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg ucguggugcu cggcagccag gagggggccg uccacacggc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc cugcaagguc cccgcgcaga uggccgugga caugcagacc     1620

cugacgccgg ugggccgccu gaucaccgcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggacaaga agaucacgca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc gaagcggaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggucuu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacgcug     1980

cucguguggc uggggcugaa caccaagaac ggcuccauca gccugaccug ccuggcgcuc     2040

gggggcguga ugaucuuccu cuccaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7932
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 3 GC

<400>  7932
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccagc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

gucgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gccgaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acaucgggca cgagaccgac gagaaccgcg ccaaggugga ggucaccccc     1080

aacuccccgc gggccgaggc cacccucggc ggguucggca gccuggggcu ggacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgcggacacg     1260

gggaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg ucguggugcu cggcagccag gagggggccg uccacaccgc gcuggccggc     1380

gcccuggagg ccgagaugga cggggcgaag ggccgccucu ucuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug cacggccgug     1500

ugcaccgccg ccaagguccc cgccgagacc cuccacggca ccgugaccgu ggagguccag     1560

uacgcgggga cggacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugaccccgg ucggccgccu gaucaccgcc aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcguc     1740

ggggacaaga agaucaccca ccacuggcac cgguccggca gcaccaucgg gaaggccuuc     1800

gaggcgacgg ugcgcggcgc caagcggaug gccgugcugg gcgacaccgc cugggacuuc     1860

gggagcgucg gcgggguguu caacucccuc ggcaagggga uccaccagau cuucggcgcc     1920

gcguucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccug     1980

cucguguggc uggggcugaa caccaagaac ggcuccauca gccucacgug ccuggcccuc     2040

gggggcguca ugaucuuccu guccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7933
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 3 GC

<400>  7933
gggagaaagc uuaccaugac cagcaucggc aucguggggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu gcagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcccgg cgcagccggc gcgccgucac ccuccccucc      360

cacagcaccc ggaagcugca gacccgcucc cagacguggc uggagagccg ggaguacacc      420

aagcaccuca ucaaggugga gaacuggauc uuccgcaacc ccggcuucgc ccugguggcg      480

gucgccaucg ccuggcugcu cggguccagc accucccaga aggugaucua ccuggugaug      540

auccugcuca ucgcccccgc cuacagcauc cggugcaucg gcgucuccaa ccgcgacuuc      600

guggagggga ugagcggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

acggucaugg cgcaggacaa gccgaccgug gacaucgagc uggugaccac cacggucucc      720

aacauggccg aggugcggag cuacugcuac gaggccucca ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaggg ggaggccuac cucgacaagc agagcgacac ccaguacgug      840

ugcaagcgga cccuggucga ccgcggcugg ggcaacgggu gcggccuguu cgggaagggc      900

ucccucguga cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc uggaguaccg gaucaugcug agcguccacg gcucccagca cagcggcaug     1020

aucgugaacg acgagaaccg cgccaaggug gaggucaccc ccaacucccc gcgggccgag     1080

gccacccucg ggggcuucgg gagccugggc cuggacugcg agccccgcac cgggcucgac     1140

uucuccgacc uguacuaccu gaccaugaac aacaagcacu ggcucgugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgcggaca ccggcacgcc gcacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agcggcagac cgucguggug     1320

cucgggagcc aggagggcgc cguccacacc gcgcuggccg gggcccugga ggccgagaug     1380

gacggcgcga aggggcgccu cuucuccggc caccugaagu gccgccugaa gauggacaag     1440

cuccggcuga agggcgugag cuacucccug ugcaccgccg ccuucacguu caccaaggug     1500

cccgccgaga cccuccacgg gaccgucacc guggaggugc aguacgccgg cacggacggg     1560

cccugcaagg uccccgcgca gauggccgug gacaugcaga cccugacccc ggugggccgc     1620

cugaucaccg ccaaccccgu caucacggag agcaccgaga acuccaagau gaugcucgag     1680

cuggacccgc ccuucggcga cagcuacauc gugaucgggg ugggcgacaa gaagaucacc     1740

caccacuggc accgguccgg gagcaccauc ggcaaggccu ucgaggccac gguccgcggg     1800

gcgaagcgga uggccgugcu gggcgacacc gccugggacu ucggcagcgu cgggggcgug     1860

uucaacuccc ucgggaaggg cauccaccag aucuucgggg ccgccuucaa gagccuguuc     1920

ggcggcaugu ccugguucag ccagauccug aucgggaccc ugcucgugug gcugggccug     1980

aacaccaaga acggguccau cagccucacg ugccuggcgc ugggcggggu gaugaucuuc     2040

cucuccaccg ccgucagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  7934
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 3 GC

<400>  7934
gggagaaagc uuaccaugac cagcaucggc aucaucgggc ugcuccugac cacggccaug       60

gccgcggaga ucacccgccg gggcuccgcc uacuacaugu accuggaccg cagcgacgcc      120

gggaaggcca ucuccuucgc caccacccuc ggcgugaaca agugccacgu ccagaucaug      180

gaccuggggc acaugugcga cgcgacgaug agcuacgagu gccccaugcu ggacgagggc      240

guggagccgg acgacgugga cugcuggugc aacaccaccu ccaccugggu cguguacggc      300

acgugccacc acaagaaggg ggagacccgg cgcagccggc gcuccgugag ccuccgguac      360

cacuacaccc gcaagcugca gacccggucc cagacguggc uggagagccg cgaguacaag      420

aagcaccuca ucauggucga gaacuggauc uuccggaacc ccggcuucgc caucgugucc      480

guggccauca ccuggcugau ggggagccug accucccaga aggucaucua ccucgugaug      540

aucgugcuga ucguccccgc cuacagcauc uccugcaucg gcgugagcaa ccgcgaccug      600

guggagggga uguccggcgg caccuggguc gacguggugc ucgagcacgg gggcugcguc      660

acggagaugg cgcaggacaa gcccaccgug gacaucgagc uggugaccau gaccgucagc      720

aacauggccg aggugcgguc cuacugcuac gaggccagcc ugagcgacau ggccuccgcc      780

agccgcugcc cgacgcaggg ggagcccucc cucgacaagc agagcgacac ccaguccgug      840

ugcaagcgga cccugggcga ccgcggcugg gggaacggcu gcgggaucuu cggcaagggg      900

agccugguga ccugcuccaa guucacgugc ugcaagaaga ugcccggcaa gagcauccag      960

cccgagaacc ucgaguaccg gaucaugcug ccggugcacg gcucccagca cagcgggaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg cgaaggugga ggugaccccc     1080

aacagccccc gggccgaggc cacccucggg ggcuucgggu cccugggccu ggacugcgag     1140

ccccgcacgg gccuggacuu cuccgaccuc uacuaccuga ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcuccccu ggcacgccgg ggccgacacc     1260

ggcacgcccc acuggaacaa caaggaggcg cugguggagu ucaaggacgc ccacgccaag     1320

cggcagaccg uggucgugcu ggggagccag gagggcgccg ugcacaccgc ccucgcgggg     1380

gcccuggagg ccgagaugga cggcgccaag ggccgccugu ucuccgggca ccucaagugc     1440

cgccugaaga uggacaagcu gcggcucaag ggcgucagcu acucccugug caccgccgcg     1500

uucaccuuca cgaaggugcc cgccgagacc cugcacggga ccgugaccgu cgaggugcag     1560

agcgccggca ccgacggccc gugcaaggug cccgcccaga uggcggucga caugcagacg     1620

cucacccccg uggggcgccu gaucaccgcc aaccccguga ucaccgaguc cacggagaac     1680

agcaagauga ugcuggagcu cgacccgccc uucggcgaca gcuacaucgu caucggggug     1740

ggcgacaaga agaucaccca ccacuggcac cgguccggga gcaccaucgg caaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gcgguccugg gggacacggc cugggacuuc     1860

ggcuccgucg ggggcguguu caacagccug gggaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg gggcaugagc ugguucuccc agauccugau cgggacccug     1980

cuggucuggc ucggccugaa caccaagaac gggagcaucu cccugaccug ccucgcccug     2040

ggcggcguga ugaucuuccu gagcacggcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7935
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 3 GC

<400>  7935
gggagaaagc uuaccaugag cgugggcauc gucgggcugc uccugaccac cgccauggcc       60

gcggagguga cgcgccgggg cuccgccuac uacauguacc uggaccgcaa cgacgccggg      120

gaggccauca gcuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac      180

cuggggcaca ugugcgacgc caccaugucc uacgagugcc cgaugcugga cgagggcgug      240

gagcccgacg acgucgacug cuggugcaac acgaccagca ccuggguggu guacgggacc      300

ugccaccaca agaaggggga ggcgcggcgc ucccggcgcg ccgucacgcu ccccagccac      360

uccacccgga agcugcagac ccgcagccag accuggcugg agucccggga guacacgaag      420

caccucaucc gcguggagaa cuggaucuuc cggaaccccg gcuucgcccu ggccgcggcc      480

gccaucgccu ggcugcucgg gagcuccacc agccagaagg ugaucuaccu ggucaugauc      540

cugcucaucg ccccggcgua cuccauccgc ugcaucggcg ugagcaaccg ggacuucgug      600

gaggggaugu ccggcggcac cugggucgac guggugcugg agcacggggg cugcgucacc      660

gugauggccc aggacaagcc cacgguggac aucgagcugg ucaccaccac cgugagcaac      720

auggccgagg ugcgcuccua cugcuacgag gccagcaucu ccgacauggc cagcgacagc      780

cggugcccca cgcaggggga ggcguaccuc gacaagcagu ccgacaccca guacgucugc      840

aagcgcaccc ugguggaccg gggcuggggc aacgggugcg gccuguucgg gaagggcagc      900

cucgugaccu gcgccaaguu cgccugcucc aagaagauga cgggcaagag cauccagccc      960

gagaaccugg aguaccgcau caugcugucc guccacggca gccagcacuc cggcaugauc     1020

gugaacgaca ccgggcacga gaccgacgag aaccgggcca aggucgagau caccccgaac     1080

agcccccgcg ccgaggcgac gcucggcggg uucggcuccc uggggcugga cugcgagccc     1140

cggaccggcc ucgacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcuc     1200

gugcacaagg agugguucca cgacaucccc cugccguggc acgccggcgc cgacaccggg     1260

acgccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgcgaagcgc     1320

cagaccgucg uggugcucgg cucccaggag ggggccgucc acaccgcccu ggccggcgcg     1380

cuggaggccg agauggacgg ggccaagggc cgccucagcu ccggccaccu gaagugccgg     1440

cugaagaugg acaagcuccg ccugaagggg gugagcuacu cccugugcac cgccgccuuc     1500

acguucacca agauccccgc ggagacccuc cacggcaccg ugaccgucga ggugcaguac     1560

gccgggacgg acggccccug caaggugccg gcccagaugg ccgucgacau gcagacccug     1620

acccccgugg gccggcugau caccgccaac cccgugauca cggagagcac cgagaacucc     1680

aagaugaugc ucgagcugga cccgcccuuc ggggacagcu acaucgucau cggcgugggg     1740

gagaagaaga ucacccacca cuggcaccgc uccggcagca ccaucgggaa ggcguucgag     1800

gccacggugc ggggcgccaa gcgcauggcc guccugggcg acaccgccug ggacuucggg     1860

agcgugggcg gggcgcucaa cucccugggc aaggggaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggcgg gauguccugg uucagccaga uccugaucgg cacccuccug     1980

auguggcugg ggcucaacac caagaacggc uccaucagcc ugaugugccu ggcccucggg     2040

ggcgugcuga ucuuccuguc cacggcgguc agcgccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  7936
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 3 GC

<400>  7936
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc ccgccggaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7937
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 3 GC

<400>  7937
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggaug uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggugaugg cccaggacaa gcccaccgug gacaucgagc uggucaccac cacggugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacccaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg ggagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucacgccg     1080

aacagccccc gcgccgaggc gacccucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacg     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca ccgugacggu cgaggugcag     1560

uacgccggga ccgacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguga ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg gaaggcguuc     1800

gaggccaccg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccaccgcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7938
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 3 GC

<400>  7938
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaugacca gcaccugggu gguguacggg      300

accugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac gcuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagaccuggc uggagucccg ggaguacacg      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

accgugaugg cccaggacaa gcccacggug gacaucgagc uggucaccac caccgugagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

agccggugcc ccacgcaggg ggaggcguac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccuggugga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga ccugcgccaa guucgccugc uccaagaaga ugacgggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccguccacg gcagccagca cuccggcaug     1020

aucgugaacg acaccgggca cgagaccgac gagaaccggg ccaaggucga gaucaccccg     1080

aacagccccc gcgccgaggc gacgcucggc ggguucggcu cccuggggcu ggacugcgag     1140

ccccggaccg gccucgacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cucgugcaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgacacc     1260

gggacgcccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgcgaag     1320

cgccagaccg ucguggugcu cggcucccag gagggggccg uccacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccgccuca gcuccggcca ccugaagugc     1440

cggcugaaga uggacaagcu ccgccugaag ggggugagcu acucccugug caccgccgcc     1500

uucacguuca ccaagauccc cgcggagacc cuccacggca ccgugaccgu cgaggugcag     1560

uacgccggga cggacggccc cugcaaggug ccggcccaga uggccgucga caugcagacc     1620

cugacccccg ugggccggcu gaucaccgcc aaccccguga ucacggagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu caucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcaccaucgg gaaggcguuc     1800

gaggccacgg ugcggggcgc caagcgcaug gccguccugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcgcu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa caccaagaac ggcuccauca gccugaugug ccuggcccuc     2040

gggggcgugc ugaucuuccu guccacggcg gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7939
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 3 GC

<400>  7939
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac cacggccaug       60

gccgcggagg ugacccgccg gggcuccgcc uacuacaugu accuggaccg caacgacgcc      120

ggggaggcca ucagcuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgccacgaug uccuacgagu gcccgaugcu ggacgagggc      240

guggagcccg acgacgucga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

acgugccacc acaagaaggg ggaggcgcgg cgcucccggc gcgccgucac ccuccccagc      360

cacuccaccc ggaagcugca gacccgcagc cagacguggc uggagucccg ggaguacacc      420

aagcaccuca uccgcgugga gaacuggauc uuccggaacc ccggcuucgc ccuggccgcg      480

gccgccaucg ccuggcugcu cgggagcucc accagccaga aggugaucua ccuggucaug      540

auccugcuca ucgccccggc guacuccauc cgcugcaucg gcgugagcaa ccgggacuuc      600

guggagggga uguccggcgg caccuggguc gacguggugc uggagcacgg gggcugcguc      660

acggccaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac cacggucagc      720

aacauggccg aggugcgcuc cuacugcuac gaggccagca ucuccgacau ggcgagcgac      780

agccggugcc ccacccaggg ggaggccuac cucgacaagc aguccgacac ccaguacgug      840

ugcaagcgca cccuggucga ccggggcugg ggcaacgggu gcggccuguu cgggaagggc      900

agccucguga cgugcgccaa guucgccugc uccaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguaccg caucaugcug uccgugcacg ggagccagca cuccggcaug     1020

cucgucaacg acaccgggca cgagaccgac gagaaccggg ccaaggugga gaucacgccg     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cuggcccaca aggagugguu ccacgacauc ccccugccgu ggcacgccgg cgccgcgacg     1260

gggacgcccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggcgg ugcacaccgc ccuggccggc     1380

gcccucgagg ccgagaugga cggggcgaag ggccgccuga gcuccggcca ccugaagugc     1440

cggcucaaga uggacaagcu gcgccugaag ggggugagcu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc gucgacggca ccgugacggu ggaggggcag     1560

uacggcggca ccgacgggcc cugcaagguc ccggcccaga uggcggugga caugcagacc     1620

cugacccccg ugggccggcu gaucacggcc aaccccguca ucaccgagag caccgagaac     1680

uccaagauga ugcucgagcu ggacccgccc uucggggaca gcuacaucgu gaucggcgug     1740

ggggagaaga agaucaccca ccacuggcac cgcuccggca gcacgaucgg caaggccuuc     1800

gaggccaccg uccgcggggc caagcggaug gcggugcugg gcgacaccgc cugggacuuc     1860

gggagcgugg gcggggcccu caacucccug ggcaagggca uccaccagau caucggggcc     1920

gcguucaaga gccuguucgg cgggaugucc ugguucagcc agauccugau cggcacccuc     1980

cugauguggc uggggcucaa cacgaagaac ggcuccauca gccugaugug ccuggcccuc     2040

ggcggggucc ugaucuuccu guccaccgcc gugagcggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7940
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 3 GC

<400>  7940
gggagaaagc uuaccauggg cgccgacgcc agcaucggga ucgugucccu gcuccugacc       60

accgucaugg cggccgagau cacgcgccgg ggcagcgccu acuacaugua ccuggaccgc      120

uccgacgccg ggaaggccau cagcuucgug accacccucg gcgugaacaa gugccacguc      180

cagaucaugg accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acacgaccag caccuggguc      300

guguacggga ccugccacca caagaagggg gaggcccggc gcucccggcg cgccgugacg      360

cuccccagcc acuccacccg gaagcugcag acccgcagcc agaccuggcu ggagucccgg      420

gaguacacga agcaccucau caaggucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cugguggcgg uggccaucgc cuggcugcuc gggagcucca ccagccagaa ggucaucuac      540

cuggugauga uccugcucau cgcccccgcc uacuccaucc ggugcaucgg cgugagcaac      600

cgcgacuucg ucgaggggau guccggcggc accugggugg acgugguccu ggagcacggg      660

ggcugcguga ccgugauggc gcaggacaag ccgacggucg acaucgagcu ggugaccacc      720

accgugagca acauggccga gguccggucc uacugcuacg aggccagcau cagcgacaug      780

gccuccgaca gccgcugccc cacgcagggg gaggccuacc ucgacaagca guccgacacc      840

caguacgugu gcaagcggac ccuggucgac cgcggcuggg gcaacgggug cggccuguuc      900

gggaagggca gccucgugac cugcgcgaag uucacgugcu ccaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguaccgg aucaugcugu ccgugcacgg cagccagcac     1020

uccggcauga ucgucaacga cgagaaccgc gccaaggugg aggugacccc caacagcccg     1080

cgggccgagg ccacccucgg gggcuucggg ucccugggcc uggacugcga gccccgcacg     1140

gggcucgacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccugccc uggcacuccg gcgccgacac cgagaccccg     1260

cacuggaaca acaaggaggc gcugguggag uucaaggacg cccacgccaa gcggcagacg     1320

guggucgugc ucggcagcca ggagggggcc gugcacaccg cccuggcggg cgcccuggag     1380

gccgagaugg acggggccaa gggccgccuc uccagcgggc accugaagug ccgccugaag     1440

auggacaagc uccggcugaa gggcgucucc uacagccugu gcaccgcggc cuucaccuuc     1500

acgaaggugc ccgccgagac ccuccacggc accgugaccg ucgaggugca guacgccggg     1560

cgcgacggcc ccugcaaggu gcccgcccag auggcggucg acaugcagac ccugacgccg     1620

guggggcggc ugaucaccgc caaccccgug aucaccgagu ccaccgagaa cagcaagaug     1680

augcucgagc uggacccgcc cuucggcgac agcuacaucg ucaucggcgu gggggacaag     1740

aagaucacgc accacuggca ccgcuccggc agcaucaucg ggaaggccuu cgaggccacc     1800

gugcggggcg ccaagcgcau ggcgguccug ggggacaccg ccugggacuu cggcuccgug     1860

ggcggggugu ucaacagccu cggcaagggg auccaccaga ucuucggcgc cgccuucaag     1920

ucccuguucg ggggcaugag cugguucucc cagauccuga ucggcacccu gcucgucugg     1980

cuggggcuga acacgaagaa cggcagcauc ucccugaccu gccuggcccu cgggggcgug     2040

augaucuucc ucagcaccgc gguguccgcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  7941
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 3 GC

<400>  7941
gggagaaagc uuaccauggg cgccgacacc agcaucggga ucaucggccu gcuccugacc       60

acggccaugg cggccgagau cacccgccgg ggguccgccu acuacaugua ccuggaccgc      120

agcgacgccg gcaaggccau cuccuucgcg accaccaugg gggugaacaa gugccacguc      180

cagaucaugg accucggcca caugugcgac gccacgauga gcuacgagug ccccaugcug      240

gacgagggcg uggagccgga cgacguggac ugcuggugca acaccaccuc caccuggguc      300

guguacggga cgugccacca caagaagggc gaggcccggc ccagccgccg ggccgugacc      360

cugcccuccc acagcacccg caagcuccag acccgguccc agacguggcu ggagagccgc      420

gaguacacca agcaccugau caaggucgag aacuggaucc uccggaaccc cggguucacc      480

guggcggccc uggccauggc cuggcugcuc ggcaacucca ccagccagcg cgugaucuac      540

cuggucauga uccugcucau cgccccggcg uacuccaucc ggugcaucgg ggugagcaac      600

cgcgacuucg uggagggcau guccggcggg acgugggucg acguggugcu ggagcacggc      660

gggugcguca ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggucaccacg      720

accgugagca acauggccga ggugcggucc uacugcuacg aggccagcau cuccgacaug      780

gccagcgaca gccgcugccc cacccagggc gaggcguacc ucgacaagca guccgacacc      840

caguacgucu gcaagcggac gcugguggac cgcggcuggg ggaacggcug cgggcuguuc      900

ggcaagggga gccucgugac cugcgccaag uucacgugcu ccaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguaccgg aucaugcugu ccguccacgg cagccagcac     1020

uccgggauga ucgugaacga caucggccac gagaccgacg agaaccgcgc caaggucgag     1080

gugaccccga acagcccccg ggccgaggcc acccucgggg gcuucggguc ccugggccug     1140

gacugcgagc cccgcaccgg ccucgacuuc agcgaccugu acuaccugac gaugaacaac     1200

aagcacuggc ucgugcacaa ggagugguuc cacgacaucc cccugccgug gcacgcgggg     1260

gccgacaccg gcacgcccca cuggaacaac aaggaggccc uggucgaguu caaggacgcc     1320

cacgccaagc ggcagaccgu ggugguccuc gggucccagg agggcgcggu gcacaccgcc     1380

cuggccgggg cccuggaggc ggagauggac ggcgccaagg gccgccucuu cagcgggcac     1440

cugaagugcc gcaucaagau ggacaagcug cggcucaagg gcguguccua cagccugugc     1500

accgccgccu ucaccuucuc caaggucccc gccgagaccc ugcacgggac cgugacggug     1560

gagguccagu acgcgggccg cgacggcccc ugcaaggugc cggcccagau ggccguggac     1620

augcagaccc ucacccccgu cgggcggcug aucaccgcca accccaucau cacggagagc     1680

accgagaacu cccgcaugau gcuggagcuc gacccgcccu ucggcgacag cuacaucgug     1740

aucggggugg gcgacaagaa gaucacccac cacuggcacc gguccgggag caccaucggc     1800

aaggccuucg aggcgacggu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcc     1860

ugggacuucg gcagcgucgg gggcguguuc aacucccugg ggaagggcgu gcaccaggug     1920

uucggcgccg cguucaagag ccucuucggg ggcauguccu gguucagcca gauccugauc     1980

gggacccugc uggucuggcu cggccugaac accaagaacg gguccaucag ccuggccugc     2040

cucgcccugg gcggcgccau gaucuuccug uccacggcgg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  7942
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 3 GC

<400>  7942
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      180

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      360

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug      840

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1080

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1140

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7943
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 3 GC

<400>  7943
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7944
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 3 GC

<400>  7944
gggagaaagc uuaccauguc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc       60

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      120

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      180

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      240

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      300

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      420

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg      540

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug      600

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac      660

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc      840

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg      900

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug      960

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1080

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1140

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1200

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1260

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1320

auggccgugc ucggggacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  7945
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 3 GC

<400>  7945
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc       60

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      120

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      180

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      300

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      420

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      540

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      600

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc      840

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag      900

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac      960

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1080

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1140

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7946
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 3 GC

<400>  7946
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggcuacgaga ccgacgagaa ccgggccaag guggagguca cgcccaacuc cccgcgcgcc      600

gaggccaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acacgggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggcaa gcuguucagc gggcaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggcgu guccuacagc cucugcacgg ccgccuucac cuucaccaag     1020

guccccgccg agacccugca cgggacggug accguggagg uccaguacgc cggcaccgac     1080

ggccccugca agauccccgu gcagauggcg guggacaugc agacccugac gccggucggg     1140

cggcucauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1260

acgcaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc caccgugcgg     1320

ggcgccaagc gcauggcggu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7947
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 3 GC

<400>  7947
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaccgu gaacgacauc      540

ggcuacgaga ccgacgagaa ccgggccaag guggagguca cgcccaacuc cccgcgcgcc      600

gaggccaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acacgggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggcaa gcuguucagc gggcaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggcgu guccuacagc cucugcacgg ccgccuucac cuucaccaag     1020

guccccgccg agacccugca cgggacggug accguggagg uccaguacgc cggcaccgac     1080

ggccccugca agauccccgu gcagauggcg guggacaugc agacccugac gccggucggg     1140

cggcucauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1260

acgcaccacu ggcaccgcag cggguccacc aucggcaagg ccuucgaggc caccgugcgg     1320

ggcgccaagc gcauggcggu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7948
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 3 GC

<400>  7948
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag      540

aaccgggcca agguggaggu cacccccaac uccccgcgcg ccgaggccac ccugggcggg      600

uucggcagcc ugggccucga cugcgagccc cggacggggc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgcgggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagacggucg uggugcuggg cagccaggag      840

ggggcggucc acaccgcccu ggccggcgcc cucgaggccg agauggacgg cgcgaagggg      900

cggcuguuca gcggccaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggg      960

guguccuaca gccucugcac cgccgccuuc accuucacga agguccccgc cgagacccug     1020

cacggcaccg ugaccgugga gguccaguac gccgggacgg acggccccug caaggugccc     1080

gcgcagaugg ccguggacau gcagacccug accccggucg gccgccucau caccgccaac     1140

cccgugauca cggaguccac cgagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggggacuccu acaucgugau cggcgucggg gacaagaaga ucacccacca cuggcaccgg     1260

agcggcucca ccaucgggaa ggccuucgag gccacggugc gcggcgcgaa gcggauggcc     1320

gugcucggcg acaccgccug ggacuucggg agcugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  7949
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 3 GC

<400>  7949
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgg cuacgagacc      540

gacgagaacc gggccaaggu ggaggugacc cccaacuccc cgcgcgccga ggccacgcuc      600

gggggcuucg gcagccuggg gcucgacugc gagccccgga ccggccugga cuucuccgac      660

cuguacuacc ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc gggggccgac accggcacgc cgcacuggaa caacaaggag      780

gcccucgugg aguucaagga cgcccacgcc aagcgccaga ccguggucgu gcuggggagc      840

caggagggcg cggugcacac cgcccuggcc ggcgcccucg aggccgagau ggacggggcg      900

aagggcaagc uguucagcgg gcaccugaag ugccggcuca agauggacaa gcugcgccug      960

aagggcgucu ccuacagccu cugcaccgcc gccuucacgu ucaccaaggu gcccgccgag     1020

acccugcacg ggaccgucac gguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggcggu ggacaugcag acccugaccc cgguggggcg gcucaucacg     1140

gccaaccccg ucaucaccga guccaccgag aacagcaaga ugaugcugga gcuggacccg     1200

cccuucggcg acuccuacau cgugaucggg gugggcgaca agaagaucac ccaccacugg     1260

caccgcagcg gguccacgau cggcaaggcc uucgaggcca ccguccgggg cgccaagcgc     1320

auggcggugc ucggggacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  7950
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 3 GC

<400>  7950
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu cccugcgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcucgag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcugguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcuggacu gcgagccccg gaccggccug      660

gacuucuccg accucuacua ccugaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccucg ccggcgcccu ggaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuca agugccgccu gaagauggac      960

aagcugcggc ucaagggcgu cagcuacucc cugugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccucac cccggugggg     1140

cgccugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcucgacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7951
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 3 GC

<400>  7951
gggagaaagc uuaccaugag caagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7952
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 3 GC

<400>  7952
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu gguaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7953
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 3 GC

<400>  7953
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggcaa gcugagcucc gggcaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cggcucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgg     1320

ggcgccaagc gcauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7954
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 3 GC

<400>  7954
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaccc agacccugac gccggugggg     1140

cgccucauca ccgccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acgcaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7955
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 3 GC

<400>  7955
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgagauc      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg cgaagcgcca gaccguggug      840

guccugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg uccaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7956
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 3 GC

<400>  7956
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgcccggc gcauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7957
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 3 GC

<400>  7957
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacaugguca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gccaaguucg cgugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag gucgagauca cccccaacuc cccgcgcgcc      600

gaggccacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1080

ggccccugca agguccccgc gcagauggcc guggacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc caccguccgc     1320

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7958
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 3 GC

<400>  7958
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaucg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7959
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 3 GC

<400>  7959
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg ggaucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7960
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 3 GC

<400>  7960
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cuggccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcucgag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcugguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccuc      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcugagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcc      600

gaggcgacgc ucgggggcuu cggcucccug gggcuggacu gcgagccgcg gaccggccug      660

gacuucuccg accucuacua ccugaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggggccg acaccggcac gccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccucg ccggcgcccu ggaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuca agugccgccu gaagauggac      960

aagcugcggc ucaagggcgu cagcuacucc cugugcaccg ccgccuucac guucaccaag     1020

aucccggccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccucac ccccgugggg     1140

cgccugauca cggccaaccc ggugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcucgacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7961
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 3 GC

<400>  7961
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga cccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cuccuacagc cucugcacgg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cgggacggug accgucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac gccggugggg     1140

cgccucauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1260

acgcaccacu ggcaccggag cggguccacc aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7962
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 3 GC

<400>  7962
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcuggc ccagauggcc gucgacaugc agacccucac ccccgugggg     1140

cgccugauca cggccaaccc ggugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcucgacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccugggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7963
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 3 GC

<400>  7963
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gaggcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7964
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 3 GC

<400>  7964
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcggccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gacgggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcgcu cgaggccgag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac cuucacgaag     1020

auccccgcgg agacccugca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg cguucgaggc cacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7965
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 3 GC

<400>  7965
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc agcaccaccg uguccaacau ggccgaggug      240

cgcagcuacu gcuacgaggc caccaucucc gacaucgcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgccgug      840

guccugggga gccaggaggg cgccgugcac accgcccugg ccggcgcgcu cgaggccgag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgcgg agacccugca cgggaccguc acgguggagg uccaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg cguucgaggc caccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7966
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 3 GC

<400>  7966
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucagcaag     1020

auccccgccg agacccugca cgggaccgug accgaggagg uccaguacgc gggcacggac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca ccgccaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccggag cggguccacc aucggcaagg ccuucgaggc gacggugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7967
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 3 GC

<400>  7967
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagc ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccucac cccggucggg     1140

cgccugauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcucgacc cgcccuucgg cgacagcuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcugggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7968
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 3 GC

<400>  7968
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcauc agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugaccgu cauggcgcag      180

gacaagccca cgguggacau cgagcucgug accaccaccg ucagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu gcacggcucc cagcacagcg ggaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcauccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg cgaagcgcca gaccguggug      840

guccuggggu cccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu guccuacagc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg uccaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga gaagaagauc     1260

acccaccacu ggcaccggag cggguccacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7969
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 3 GC

<400>  7969
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ucaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu gagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccguc acgguggagg ugcaguacgc cggcaccgac     1080

ggccccugca agguccccgc gcagauggcc guggacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacg aucggcaagg ccuucgaggc caccguccgc     1320

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7970
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 3 GC

<400>  7970
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcgccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg cguucgaggc cacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7971
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 3 GC

<400>  7971
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac accggggccg acacgggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgcggugcac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcacgg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cgggacggug accgucgagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc gcagauggcc gucgacaugc agacccugac gccggugggg     1140

cgccucauca ccgccaaccc cgugaucacc gagggcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg ggacuccuac aucgucaucg gcguggggga gaagaagauc     1260

acgcaccacu ggcaccggag cggcuccacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggggcgaagc ggauggccgu ccucggcgac accgccuggg acuucgggag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7972
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 3 GC

<400>  7972
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgc gauggcccag      180

gacaagccca cgguggacau cgagcucgug accaccaccg ucagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg cguaccugga caagcagagc gacacccagu acgugugcaa gcgcacccug      360

gucgaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu gcacggcucc cagcacagcg ggaugcuggu caacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcg      600

gaggccacgc ucgggggcuu cggcagccuc gggcuggacu gcgagccccg gaccggccug      660

gacuucuccg accucuacua ccugaccaug aacaacaagc acuggcuggc ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggggccg cgaccggcac gccgcacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgccguccac accgcgcucg ccggcgcccu ggaggccgag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuca agugccgccu gaagauggac      960

aagcugcggc ucaagggcgu gagcuacucc cugugcaccg cggccuucac guucaccaag     1020

auccccgccg agaccgugga cgggaccguc acgguggagg gccaguacgg cgggaccgac     1080

ggccccugca agguccccgc ccagauggcc guggacaugc agacccugac cccggugggg     1140

cgccucauca cggcgaaccc cgucaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cggcagcacg aucgggaagg ccuucgaggc caccguccgc     1320

ggcgccaagc ggauggcggu gcucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7973
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 3 GC

<400>  7973
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagcccg ccguggacau cgagcucguc acgaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      300

cagggcgagg cguaccugga caagcagagc gacacgcagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacg      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcg      600

gaggccaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gacgggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcgcu cgaggccgag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac cuucacgaag     1020

auccccgcgg agacccugca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg cguucgaggc cacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7974
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 3 GC

<400>  7974
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug aacaaccggg acuucgugga ggggaugagc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg uguccaacau ggccgaggug      240

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacg      300

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugaccugc      420

gcgaaguucg ccugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag      480

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcucccug gggcucgacu gcgagccccg gaccggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcuggggu cccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cuccuacagc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccggag cggguccacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7975
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 3 GC

<400>  7975
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcgccg ccgccuucac cuucacgaag     1020

auccccgcgg agacccugca cgggaccgug accgucgagg ugcaguacgc cggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg cguucgaggc cacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgcguggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7976
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 3 GC

<400>  7976
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gacgggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gacggugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac cuucacgaag     1020

auccccgccg agacccugca cgggaccgug accgucgagg ugcaguacgc gggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1260

acccaccacu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7977
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 3 GC

<400>  7977
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguucg ccugcagcaa gaagaugacg ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacuc cccgcgcgcc      600

gaggcgacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcgcca gaccgugguc      840

gugcugggga gccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg gcugagcucc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu cagcuacucc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca cggccaaccc cgugaucacc gagagcaccg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacagcuac aucgucaucg gggugggcga caagaagauc     1260

acccaccacu gguaccgguc cgggagcacg aucggcaagg ccuucgaggc gaccgugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7978
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 3 GC

<400>  7978
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgggccaag guggagguca cgcccaacuc cccgcgcgcc      600

gaggccaccc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acacgggcac cccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcuguucagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu guccuacagc cucugcacgg ccgccuucac cuucaccaag     1020

guccccgccg agacccugca cgggacggug accguggagg uccaguacgc cggcaccgac     1080

ggccccugca aggugcccgc gcagauggcc guggacaugc agacccugac gccggucggg     1140

cgccucauca ccgccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1260

acgcaccacu ggcaccggag cggguccacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7979
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 3 GC

<400>  7979
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa ccgggccaag guggagguca cccccaacuc cccgcgcgcc      600

gaggccacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcuguucagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu guccuacagc cucugcaccg ccgccuucac guucaccaag     1020

guccccgccg agacccugca cgggaccgug acgguggagg uccaguacgc cggcaccgac     1080

ggccccugca aggugcccgc gcagauggcc guggacaugc agacccugac cccggucggg     1140

cgccucauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gggucggcga caagaagauc     1260

acccaccacu ggcaccggag cggguccacg aucggcaagg ccuucgaggc caccgugcgc     1320

ggcgcgaagc ggauggccgu gcucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7980
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 3 GC

<400>  7980
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa ccgggccaag guggagguca cccccaacuc cccgcgcgcc      600

gaggccacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg gaccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcgcca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg gcuguucagc gggcaccuga agugccgccu caagauggac      960

aagcugcggc ugaagggcgu guccuacagc cucugcaccg ccgucugcac ggccgccaag     1020

gugcccgccg agacccugca cgggaccgug accgucgagg ugcaguacgc gggcacggac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cgccucauca ccgccaaccc cgugaucacg gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga caagaagauc     1260

acccaccacu ggcaccggag cggguccacc aucggcaagg ccuucgaggc gacggugcgc     1320

ggcgccaagc ggauggccgu ccucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7981
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 3 GC

<400>  7981
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccgcug caucggcgug agcaaccggg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgcuccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcgcacccug      360

guggaccggg gcugggggaa cggcugcggc cucuucggga aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccgcauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacgag      540

aaccgggcca agguggaggu cacccccaac uccccgcgcg ccgaggccac ccugggcggg      600

uucggcagcc ugggccucga cugcgagccc cggacggggc uggacuucuc cgaccuguac      660

uaccucacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acagcggcgc ggacaccgag accccgcacu ggaacaacaa ggaggcccuc      780

guggaguuca aggacgccca cgccaagcgc cagacggucg uggugcuggg gagccaggag      840

ggcgccgucc acaccgcgcu ggccggggcc cucgaggccg agauggacgg cgccaagggc      900

cggcugucca gcgggcaccu gaagugccgc cucaagaugg acaagcugcg gcugaagggc      960

guguccuaca gccucugcac cgcggccuuc accuucacga agguccccgc cgagacccug     1020

cacgggaccg ugaccgugga gguccaguac gccggccgcg acgggcccug caaggugccc     1080

gcccagaugg cgguggacau gcagacgcug accccggucg gccggcucau caccgccaac     1140

cccgugauca ccgaguccac ggagaacagc aagaugaugc uggagcugga cccgcccuuc     1200

ggcgacuccu acaucgugau cggggucggc gacaagaaga ucacccacca cuggcaccgc     1260

agcgggucca ucaucggcaa ggccuucgag gccaccgugc gcggggccaa gcggauggcg     1320

gugcucggcg acaccgccug ggacuucggc agcugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  7982
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 3 GC

<400>  7982
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugaucgu gcucaucgug       60

cccgccuacu ccaucagcug caucggcguc uccaaccgcg accuggugga ggggaugagc      120

ggcgggaccu ggguggacgu cgugcuggag cacggcgggu gcgugaccga gauggcccag      180

gacaagccca cggucgacau cgagcucgug accaugaccg uguccaacau ggcggagguc      240

cggagcuacu gcuacgaggc cucccugagc gacauggccu ccgccagccg cugccccacc      300

cagggcgagc ccucccugga caagcagagc gacacgcagu ccgugugcaa gcggacccuc      360

ggcgaccgcg gguggggcaa cgggugcggc aucuucggga agggcagccu ggugaccugc      420

uccaaguuca cgugcugcaa gaagaugccc ggcaagagca uccagccgga gaaccuggag      480

uaccggauca ugcuccccgu ccacgggucc cagcacagcg gcaugaucgu gaacgacauc      540

gggcacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacag cccccgggcg      600

gaggccaccc ugggcggcuu cgggucccug ggccucgacu gcgagccgcg caccgggcug      660

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccgggac gccccacugg      780

aacaacaagg aggcgcucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug      840

gugcugggcu cccaggaggg cgccguccac accgcccugg cgggggcccu cgaggccgag      900

auggacggcg ccaaggggcg ccuguucagc ggccaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggggu guccuacagc cucugcaccg ccgcguucac cuucaccaag     1020

gucccggccg agacgcugca cggcaccgug accguggagg uccaguccgc cggcaccgac     1080

gggcccugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggc     1140

cggcucauca ccgcgaaccc ggugaucacc gagagcacgg agaacuccaa gaugaugcug     1200

gagcuggacc cgcccuucgg ggacagcuac aucgugaucg gcgucgggga caagaagauc     1260

acccaccacu ggcaccgcuc cggcagcacc aucggcaagg ccuucgaggc caccgugcgc     1320

ggggccaagc ggauggccgu gcucggcgac acggcguggg acuucggguc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7983
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 3 GC

<400>  7983
gggagaaagc uuaccaugcg ccagaaggug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuaca gcauccggug caucggcgug uccaaccgcg acuucgugga ggggaugagc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg uguccaacau ggccgaggug      240

cggagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg cugccccacg      300

cagggcgagg ccuaccugga caagcagucc gacacccagu acgucugcaa gcggacccug      360

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggagccu ggugaccugc      420

gcgaaguucg ccugcuccaa gaagaugacg ggcaagagca uccagcccga gaaccuggag      480

uaccggauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag guggagauca cccccaacag cccgcgggcc      600

gaggcgacgc ucgggggcuu cggcucccug gggcucgacu gcgagccccg caccggccug      660

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg cgaagcggca gaccgugguc      840

gugcuggggu cccaggaggg cgccgugcac accgcccugg ccggcgcccu cgaggcggag      900

auggacgggg ccaagggccg ccugagcagc gggcaccuga agugccggcu caagauggac      960

aagcugcgcc ugaagggcgu cuccuacagc cucugcaccg ccgccuucac guucaccaag     1020

auccccgccg agacccugca cgggaccgug acggucgagg ugcaguacgc gggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc gucgacaugc agacccugac cccggugggg     1140

cggcucauca cggccaaccc cgugaucacc gaguccaccg agaacagcaa gaugaugcug     1200

gagcuggacc cgcccuucgg cgacuccuac aucgucaucg gggugggcga gaagaagauc     1260

acccaccacu ggcaccgcag cggguccacg aucggcaagg ccuucgaggc gaccgugcgg     1320

ggcgccaagc gcauggccgu ccucggggac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7984
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 3 GC

<400>  7984
gggagaaagc uuaccaugag ccagcgcgug aucuaccugg ucaugauccu ccugaucgcc       60

cccgccuacu ccauccggug caucggcgug agcaaccgcg acuucgugga ggggaugucc      120

ggcgggaccu gggucgacgu ggugcuggag cacggcgggu gcgucaccgu gauggcgcag      180

gacaagccca cgguggacau cgagcucguc accaccaccg ugagcaacau ggccgaggug      240

cgguccuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg cugccccacg      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgucugcaa gcggacccug      360

guggaccgcg gcugggggaa cggcugcggg cuguucggca aggggucccu ggugaccugc      420

gcgaaguuca cgugcagcaa gaagaugacc ggcaagucca uccagcccga gaaccuggag      480

uaccggauca ugcucagcgu ccacggcucc cagcacagcg ggaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag guggagguca cccccaacuc cccgcgggcc      600

gaggccacgc ucgggggcuu cggcagccug gggcucgacu gcgagccccg caccggccug      660

gacuucuccg accuguacua ccucaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gcgggggccg acaccggcac gccgcacugg      780

aacaacaagg aggcccucgu ggaguucaag gacgcccacg ccaagcggca gaccgucgug      840

gugcugggga gccaggaggg cgcgguccac accgcccugg ccggcgcccu cgaggccgag      900

auggacgggg cgaagggccg ccuguucagc gggcaccuga agugccggau caagauggac      960

aagcuccgcc ugaagggcgu guccuacagc cugugcaccg ccgccuucac guucuccaag     1020

guccccgccg agacccucca cgggaccgug accguggagg uccaguacgc cggccgggac     1080

ggccccugca aggugcccgc gcagauggcc guggacaugc agacgcugac cccggucggg     1140

cgccugauca ccgccaaccc caucaucacc gagagcacgg agaacucccg gaugaugcuc     1200

gagcuggacc cgcccuucgg cgacagcuac aucgugaucg gggugggcga caagaagauc     1260

acccaccacu ggcaccgcuc cgggagcacc aucggcaagg ccuucgaggc caccguccgg     1320

ggcgcgaagc gcauggccgu gcugggggac acggccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7985
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  7985
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7986
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 3 opt

<400>  7986
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacacc      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacgcca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7987
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 3 opt

<400>  7987
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc     1080

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     2040

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcuccguc gacuucucca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  7988
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 3 opt

<400>  7988
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  7989
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  7989
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7990
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 3 opt

<400>  7990
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7991
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 3 opt

<400>  7991
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  7992
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 3 opt

<400>  7992
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7993
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 3 opt

<400>  7993
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7994
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 3 opt

<400>  7994
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7995
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 3 opt

<400>  7995
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc      540

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc      600

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      660

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      720

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc      840

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc      900

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc      960

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1020

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1140

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1200

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1260

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1320

auggccgucc ucggcgacac cgccugggac uucggcuccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  7996
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 3 opt

<400>  7996
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  7997
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       3 opt

<400>  7997
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuc cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7998
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 3
       opt

<400>  7998
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuc cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  7999
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 3 
       opt

<400>  7999
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuc cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8000
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F399S) mRNA Design 3 
       opt

<400>  8000
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuc cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8001
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       3 opt

<400>  8001
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucguccagg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8002
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 3
       opt

<400>  8002
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucguccagg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8003
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 3 
       opt

<400>  8003
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucguccagg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8004
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8004
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8005
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8005
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8006
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 3 opt

<400>  8006
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc     1800

aagggcgccc agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc     1860

ggcgucuuca acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc     1920

cucuucggcg gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug     1980

ggcgucaacg cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc     2040

gucuuccucg ccaccaacgu ccacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8007
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8007
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8008
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  8008
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8009
<211>  2362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8009
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcgccg cggcgccgac       60

accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc      120

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      180

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      240

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      300

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      360

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      420

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      480

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      540

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      600

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      660

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      720

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      780

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      840

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      900

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac     1020

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1080

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1140

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1200

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1260

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1380

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1440

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1500

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1620

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1680

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1740

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1800

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1860

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1920

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1980

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     2040

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2100

cucuccaccg ccgucuccgc cgacgucggc ugcuccgucg acuucuccaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2340

ucuuuucaga gccaccagaa uu                                              2362


<210>  8010
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8010
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcgccgcgg cgccgacacc       60

uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc      120

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      180

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      240

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      300

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      360

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      420

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      480

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      540

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      600

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      660

accugggucg acaucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      720

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      780

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      840

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      900

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      960

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc     1020

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1080

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1140

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1200

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1260

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1380

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1440

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1500

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1620

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1680

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1740

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1800

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1920

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1980

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     2040

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2100

uccaccgccg ucuccgccga cgucggcugc uccgucgacu ucuccaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  8011
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8011
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc gccgcggcgc cgacaccucc       60

gucggcaucg ucggccuccu ccucaccacc gccauggccg ccgaggucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcucc gucgacuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8012
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8012
gggagaaagc uuaccauggc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc       60

ggcaucgucg gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc      120

gccuacuaca uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc      180

cucggcauga acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc      240

auguccuacg agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg      300

ugcaacacca ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc      360

cgccgcuccc gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      420

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      480

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      540

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      600

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      660

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      720

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      780

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      840

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      900

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug     1020

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc     1080

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     2040

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcuccguc gacuucucca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8013
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8013
gggagaaagc uuaccaugcg caaggagaag aagcgccgcg gcgccgacac cuccgucggc       60

aucgucggcc uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc      120

uacuacaugu accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc      180

ggcaugaaca agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc      300

aacaccaccu ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc      360

cgcucccgcc gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      480

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      540

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      600

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      660

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      780

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      840

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      900

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      960

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc     1020

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac     1080

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc     1140

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1200

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1260

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1380

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1440

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1500

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1620

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1680

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1740

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1800

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1860

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1980

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     2040

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     2100

gucuccgccg acgucggcug cuccgucgac uucuccaagu gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2220

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  8014
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8014
gggagaaagc uuaccaugaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8015
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8015
gggagaaagc uuaccaugga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgacgucg gcugcuccgu cgacuucucc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  8016
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8016
gggagaaagc uuaccaugaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gacgucggcu gcuccgucga cuucuccaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  8017
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8017
gggagaaagc uuaccaugaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugcu ccgucgacuu cuccaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8018
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8018
gggagaaagc uuaccaugcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugcuccg ucgacuucuc caagugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8019
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8019
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcuccgucg acuucuccaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8020
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8020
gggagaaagc uuaccauggg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

uccgucgacu ucuccaagug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8021
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8021
gggagaaagc uuaccauggc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgacgu cggcugcucc     2100

gucgacuucu ccaagugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8022
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8022
gggagaaagc uuaccaugga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgacgucgg cugcuccguc     2100

gacuucucca agugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8023
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8023
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cuccgucgac     2100

uucuccaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8024
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8024
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cuccgucgac     2100

uucuccaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8025
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8025
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccgc cgucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcuccgucg acuucuccaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8026
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8026
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcggcgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcucc gucgacuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8027
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8027
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcgccgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcucc gucgacuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8028
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8028
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8029
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8029
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcgccg cggcgccgac       60

accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc      120

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      180

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      240

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      300

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      360

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      420

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      480

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      540

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      600

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      660

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      720

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      780

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      840

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      900

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac     1020

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1080

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1140

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1200

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1260

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1380

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1440

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1500

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1620

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1680

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1740

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1800

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1860

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1920

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1980

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     2040

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2100

cucuccaccg ccgucuccgc cgacgucggc ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  8030
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8030
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcgccgcgg cgccgacacc       60

uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc      120

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      180

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      240

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      300

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      360

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      420

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      480

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      540

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      600

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      660

accugggucg acaucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      720

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      780

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      840

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      900

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      960

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc     1020

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1080

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1140

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1200

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1260

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1380

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1440

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1500

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1620

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1680

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1740

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1800

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1920

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1980

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     2040

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2100

uccaccgccg ucuccgccga cgucggcugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  8031
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8031
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc gccgcggcgc cgacaccucc       60

gucggcaucg ucggccuccu ccucaccacc gccauggccg ccgaggucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8032
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8032
gggagaaagc uuaccauggc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc       60

ggcaucgucg gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc      120

gccuacuaca uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc      180

cucggcauga acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc      240

auguccuacg agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg      300

ugcaacacca ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc      360

cgccgcuccc gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      420

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      480

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      540

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      600

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      660

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      720

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      780

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      840

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      900

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug     1020

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc     1080

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     2040

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8033
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8033
gggagaaagc uuaccaugcg caaggagaag aagcgccgcg gcgccgacac cuccgucggc       60

aucgucggcc uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc      120

uacuacaugu accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc      180

ggcaugaaca agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc      300

aacaccaccu ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc      360

cgcucccgcc gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      480

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      540

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      600

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      660

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      780

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      840

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      900

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      960

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc     1020

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac     1080

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc     1140

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1200

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1260

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1380

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1440

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1500

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1620

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1680

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1740

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1800

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1860

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1980

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     2040

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     2100

gucuccgccg acgucggcug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8034
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8034
gggagaaagc uuaccaugaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8035
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8035
gggagaaagc uuaccaugga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgacgucg gcugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8036
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8036
gggagaaagc uuaccaugaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gacgucggcu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8037
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8037
gggagaaagc uuaccaugaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

gucggcugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8038
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8038
gggagaaagc uuaccaugcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacguc     2100

ggcugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8039
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8039
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8040
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8040
gggagaaagc uuaccauggg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga cgucggcugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8041
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8041
gggagaaagc uuaccauggc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgacgu cggcugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8042
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8042
gggagaaagc uuaccaugga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgacgucgg cugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8043
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8043
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8044
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8044
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acgucggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8045
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8045
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccgc cgucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacgucggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8046
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8046
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcggcgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8047
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8047
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcgccgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8048
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8048
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8049
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8049
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcgccg cggcgccgac       60

accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc      120

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      180

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      240

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      300

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      360

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      420

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      480

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      540

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      600

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      660

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      720

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      780

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      840

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      900

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac     1020

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1080

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1140

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1200

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1260

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1380

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1440

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1500

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1620

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1680

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1740

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1800

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1860

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1920

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1980

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     2040

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2100

cucuccaccg ccgucuccgc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8050
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8050
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcgccgcgg cgccgacacc       60

uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc      120

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      180

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      240

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      300

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      360

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      420

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      480

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      540

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      600

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      660

accugggucg acaucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      720

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      780

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      840

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      900

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      960

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc     1020

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1080

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1140

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1200

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1260

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1380

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1440

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1500

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1620

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1680

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1740

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1800

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1920

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1980

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     2040

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2100

uccaccgccg ucuccgccga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8051
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8051
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc gccgcggcgc cgacaccucc       60

gucggcaucg ucggccuccu ccucaccacc gccauggccg ccgaggucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8052
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8052
gggagaaagc uuaccauggc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc       60

ggcaucgucg gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc      120

gccuacuaca uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc      180

cucggcauga acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc      240

auguccuacg agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg      300

ugcaacacca ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc      360

cgccgcuccc gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      420

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      480

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      540

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      600

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      660

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      720

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      780

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      840

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      900

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug     1020

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc     1080

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     2040

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     2100

gccgucuccg ccgacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8053
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8053
gggagaaagc uuaccaugcg caaggagaag aagcgccgcg gcgccgacac cuccgucggc       60

aucgucggcc uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc      120

uacuacaugu accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc      180

ggcaugaaca agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc      300

aacaccaccu ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc      360

cgcucccgcc gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      480

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      540

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      600

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      660

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      780

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      840

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      900

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      960

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc     1020

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac     1080

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc     1140

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1200

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1260

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1380

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1440

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1500

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1620

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1680

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1740

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1800

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1860

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1980

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     2040

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     2100

gucuccgccg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8054
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8054
gggagaaagc uuaccaugaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccgacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8055
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8055
gggagaaagc uuaccaugga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8056
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8056
gggagaaagc uuaccaugaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8057
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8057
gggagaaagc uuaccaugaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccgac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8058
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8058
gggagaaagc uuaccaugcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8059
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8059
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8060
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8060
gggagaaagc uuaccauggg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8061
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8061
gggagaaagc uuaccauggc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8062
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8062
gggagaaagc uuaccaugga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccgacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  8063
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8063
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8064
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8064
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8065
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8065
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccgc cgucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8066
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8066
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcggcgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8067
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8067
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcgccgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8068
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8068
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8069
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8069
gggagaaagc uuaccaugau caacgcccgc aaggagaaga agcgccgcgg cgccgacacc       60

uccgucggca ucgucggccu ccuccucacc accgccaugg ccgccgaggu cacccgccgc      120

ggcuccgccu acuacaugua ccucgaccgc aacgacgccg gcgaggccau cuccuucccc      180

accacccucg gcaugaacaa gugcuacauc cagaucaugg accucggcca caugugcgac      240

gccaccaugu ccuacgagug ccccaugcuc gacgagggcg ucgagcccga cgacgucgac      300

ugcuggugca acaccaccuc caccuggguc gucuacggca ccugccacca caagaagggc      360

gaggcccgcc gcucccgccg cgccgucacc cuccccuccc acuccacccg caagcuccag      420

acccgcuccc agaccuggcu cgagucccgc gaguacacca agcaccucau ccgcgucgag      480

aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg ccgccaucgc cuggcuccuc      540

ggcuccucca ccucccagaa ggucaucuac cucgucauga uccuccucau cgcccccgcc      600

uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg ucgagggcau guccggcggc      660

accugggucg acaucguccu cgagcacggc ggcugcguca ccgucauggc ccaggacaag      720

cccaccgucg acaucgagcu cgucaccacc accgucucca acauggccga gguccgcucc      780

uacugcuacg aggccuccau cuccgacaug gccuccgacu cccgcugccc cacccagggc      840

gaggccuacc ucgacaagca guccgacacc caguacgucu gcaagcgcac ccucgucgac      900

cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu cccucgucac cugcgccaag      960

uucgccugcu ccaagaagau gaccggcaag uccauccagc ccgagaaccu cgaguaccgc     1020

aucaugcucu ccguccacgg cucccagcac uccggcauga ucgucaacga caccggccac     1080

gagaccgacg agaaccgcgc caaggucgag aucaccccca acuccccccg cgccgaggcc     1140

acccucggcg gcuucggcuc ccucggccuc gacugcgagc cccgcaccgg ccucgacuuc     1200

uccgaccucu acuaccucac caugaacaac aagcacuggc ucguccacaa ggagugguuc     1260

cacgacaucc cccuccccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ucgucgaguu caaggacgcc cacgccaagc gccagaccgu cgucguccuc     1380

ggcucccagg agggcgccgu ccacaccgcc cucgccggcg cccucgaggc cgagauggac     1440

ggcgccaagg gccgccucuc cuccggccac cucaagugcc gccucaagau ggacaagcuc     1500

cgccucaagg gcgucuccua cucccucugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc uccacggcac cgucaccguc gagguccagu acgccggcac cgacggcccc     1620

ugcaaggucc ccgcccagau ggccgucgac augcagaccc ucacccccgu cggccgccuc     1680

aucaccgcca accccgucau caccgagucc accgagaacu ccaagaugau gcucgagcuc     1740

gacccccccu ucggcgacuc cuacaucguc aucggcgucg gcgagaagaa gaucacccac     1800

cacuggcacc gcuccggcuc caccaucggc aaggccuucg aggccaccgu ccgcggcgcc     1860

aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg gcuccgucgg cggcgcccuc     1920

aacucccucg gcaagggcau ccaccagauc uucggcgccg ccuucaaguc ccucuucggc     1980

ggcauguccu gguucuccca gauccucauc ggcacccucc ucauguggcu cggccucaac     2040

accaagaacg gcuccaucuc ccucaugugc cucgcccucg gcggcguccu caucuuccuc     2100

uccaccgccg ucuccgccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8070
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8070
gggagaaagc uuaccaugau caucaacgcc cgcaaggaga agaagcgccg cggcgccgac       60

accuccgucg gcaucgucgg ccuccuccuc accaccgcca uggccgccga ggucacccgc      120

cgcggcuccg ccuacuacau guaccucgac cgcaacgacg ccggcgaggc caucuccuuc      180

cccaccaccc ucggcaugaa caagugcuac auccagauca uggaccucgg ccacaugugc      240

gacgccacca uguccuacga gugccccaug cucgacgagg gcgucgagcc cgacgacguc      300

gacugcuggu gcaacaccac cuccaccugg gucgucuacg gcaccugcca ccacaagaag      360

ggcgaggccc gccgcucccg ccgcgccguc acccuccccu cccacuccac ccgcaagcuc      420

cagacccgcu cccagaccug gcucgagucc cgcgaguaca ccaagcaccu cauccgcguc      480

gagaacugga ucuuccgcaa ccccggcuuc gcccucgccg ccgccgccau cgccuggcuc      540

cucggcuccu ccaccuccca gaaggucauc uaccucguca ugauccuccu caucgccccc      600

gccuacucca uccgcugcau cggcgucucc aaccgcgacu ucgucgaggg cauguccggc      660

ggcaccuggg ucgacaucgu ccucgagcac ggcggcugcg ucaccgucau ggcccaggac      720

aagcccaccg ucgacaucga gcucgucacc accaccgucu ccaacauggc cgagguccgc      780

uccuacugcu acgaggccuc caucuccgac auggccuccg acucccgcug ccccacccag      840

ggcgaggccu accucgacaa gcaguccgac acccaguacg ucugcaagcg cacccucguc      900

gaccgcggcu ggggcaacgg cugcggccuc uucggcaagg gcucccucgu caccugcgcc      960

aaguucgccu gcuccaagaa gaugaccggc aaguccaucc agcccgagaa ccucgaguac     1020

cgcaucaugc ucuccgucca cggcucccag cacuccggca ugaucgucaa cgacaccggc     1080

cacgagaccg acgagaaccg cgccaagguc gagaucaccc ccaacucccc ccgcgccgag     1140

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1200

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1260

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1380

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1440

gacggcgcca agggccgccu cuccuccggc caccucaagu gccgccucaa gauggacaag     1500

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1620

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1680

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1740

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgagaa gaagaucacc     1800

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1860

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcgcc     1920

cucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1980

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucaugug gcucggccuc     2040

aacaccaaga acggcuccau cucccucaug ugccucgccc ucggcggcgu ccucaucuuc     2100

cucuccaccg ccgucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8071
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8071
gggagaaagc uuaccaugaa cgcccgcaag gagaagaagc gccgcggcgc cgacaccucc       60

gucggcaucg ucggccuccu ccucaccacc gccauggccg ccgaggucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8072
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8072
gggagaaagc uuaccauggc ccgcaaggag aagaagcgcc gcggcgccga caccuccguc       60

ggcaucgucg gccuccuccu caccaccgcc auggccgccg aggucacccg ccgcggcucc      120

gccuacuaca uguaccucga ccgcaacgac gccggcgagg ccaucuccuu ccccaccacc      180

cucggcauga acaagugcua cauccagauc auggaccucg gccacaugug cgacgccacc      240

auguccuacg agugccccau gcucgacgag ggcgucgagc ccgacgacgu cgacugcugg      300

ugcaacacca ccuccaccug ggucgucuac ggcaccugcc accacaagaa gggcgaggcc      360

cgccgcuccc gccgcgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      420

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      480

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      540

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      600

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      660

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      720

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      780

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      840

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      900

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      960

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug     1020

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc     1080

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     2040

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     2100

gccgucuccg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8073
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8073
gggagaaagc uuaccaugcg caaggagaag aagcgccgcg gcgccgacac cuccgucggc       60

aucgucggcc uccuccucac caccgccaug gccgccgagg ucacccgccg cggcuccgcc      120

uacuacaugu accucgaccg caacgacgcc ggcgaggcca ucuccuuccc caccacccuc      180

ggcaugaaca agugcuacau ccagaucaug gaccucggcc acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu cgacgagggc gucgagcccg acgacgucga cugcuggugc      300

aacaccaccu ccaccugggu cgucuacggc accugccacc acaagaaggg cgaggcccgc      360

cgcucccgcc gcgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      420

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      480

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      540

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      600

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      660

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      720

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      780

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      840

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      900

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      960

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc     1020

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac     1080

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc     1140

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc     1200

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc     1260

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1380

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1440

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1500

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1620

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1680

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1740

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1800

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1860

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1980

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     2040

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     2100

gucuccgccu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8074
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8074
gggagaaagc uuaccaugaa ggagaagaag cgccgcggcg ccgacaccuc cgucggcauc       60

gucggccucc uccucaccac cgccauggcc gccgagguca cccgccgcgg cuccgccuac      120

uacauguacc ucgaccgcaa cgacgccggc gaggccaucu ccuuccccac cacccucggc      180

augaacaagu gcuacaucca gaucauggac cucggccaca ugugcgacgc caccaugucc      240

uacgagugcc ccaugcucga cgagggcguc gagcccgacg acgucgacug cuggugcaac      300

accaccucca ccugggucgu cuacggcacc ugccaccaca agaagggcga ggcccgccgc      360

ucccgccgcg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      420

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      480

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      540

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      600

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      660

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      720

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      780

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      840

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      900

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      960

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc     1020

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag     1080

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     2100

uccgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8075
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8075
gggagaaagc uuaccaugga gaagaagcgc cgcggcgccg acaccuccgu cggcaucguc       60

ggccuccucc ucaccaccgc cauggccgcc gaggucaccc gccgcggcuc cgccuacuac      120

auguaccucg accgcaacga cgccggcgag gccaucuccu uccccaccac ccucggcaug      180

aacaagugcu acauccagau cauggaccuc ggccacaugu gcgacgccac cauguccuac      240

gagugcccca ugcucgacga gggcgucgag cccgacgacg ucgacugcug gugcaacacc      300

accuccaccu gggucgucua cggcaccugc caccacaaga agggcgaggc ccgccgcucc      360

cgccgcgccg ucacccuccc cucccacucc acccgcaagc uccagacccg cucccagacc      420

uggcucgagu cccgcgagua caccaagcac cucauccgcg ucgagaacug gaucuuccgc      480

aaccccggcu ucgcccucgc cgccgccgcc aucgccuggc uccucggcuc cuccaccucc      540

cagaagguca ucuaccucgu caugauccuc cucaucgccc ccgccuacuc cauccgcugc      600

aucggcgucu ccaaccgcga cuucgucgag ggcauguccg gcggcaccug ggucgacauc      660

guccucgagc acggcggcug cgucaccguc auggcccagg acaagcccac cgucgacauc      720

gagcucguca ccaccaccgu cuccaacaug gccgaggucc gcuccuacug cuacgaggcc      780

uccaucuccg acauggccuc cgacucccgc ugccccaccc agggcgaggc cuaccucgac      840

aagcaguccg acacccagua cgucugcaag cgcacccucg ucgaccgcgg cuggggcaac      900

ggcugcggcc ucuucggcaa gggcucccuc gucaccugcg ccaaguucgc cugcuccaag      960

aagaugaccg gcaaguccau ccagcccgag aaccucgagu accgcaucau gcucuccguc     1020

cacggcuccc agcacuccgg caugaucguc aacgacaccg gccacgagac cgacgagaac     1080

cgcgccaagg ucgagaucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc     1140

ggcucccucg gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac     1200

cucaccauga acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc     1320

gaguucaagg acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc     1380

gccguccaca ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc     1440

cucuccuccg gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc     1500

uccuacuccc ucugcaccgc cgccuucacc uucaccaaga uccccgccga gacccuccac     1560

ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gguccccgcc     1620

cagauggccg ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc     1680

gucaucaccg aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc     1740

gacuccuaca ucgucaucgg cgucggcgag aagaagauca cccaccacug gcaccgcucc     1800

ggcuccacca ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc     1860

cucggcgaca ccgccuggga cuucggcucc gucggcggcg cccucaacuc ccucggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc     1980

ucccagaucc ucaucggcac ccuccucaug uggcucggcc ucaacaccaa gaacggcucc     2040

aucucccuca ugugccucgc ccucggcggc guccucaucu uccucuccac cgccgucucc     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8076
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8076
gggagaaagc uuaccaugaa gaagcgccgc ggcgccgaca ccuccgucgg caucgucggc       60

cuccuccuca ccaccgccau ggccgccgag gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8077
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8077
gggagaaagc uuaccaugaa gcgccgcggc gccgacaccu ccgucggcau cgucggccuc       60

cuccucacca ccgccauggc cgccgagguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8078
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8078
gggagaaagc uuaccaugcg ccgcggcgcc gacaccuccg ucggcaucgu cggccuccuc       60

cucaccaccg ccauggccgc cgaggucacc cgccgcggcu ccgccuacua cauguaccuc      120

gaccgcaacg acgccggcga ggccaucucc uuccccacca cccucggcau gaacaagugc      180

uacauccaga ucauggaccu cggccacaug ugcgacgcca ccauguccua cgagugcccc      240

augcucgacg agggcgucga gcccgacgac gucgacugcu ggugcaacac caccuccacc      300

ugggucgucu acggcaccug ccaccacaag aagggcgagg cccgccgcuc ccgccgcgcc      360

gucacccucc ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag      420

ucccgcgagu acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc      480

uucgcccucg ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc      540

aucuaccucg ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc      600

uccaaccgcg acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag      660

cacggcggcu gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc      720

accaccaccg ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc      780

gacauggccu ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc      840

gacacccagu acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc      900

cucuucggca agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc      960

ggcaagucca uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc     1020

cagcacuccg gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag     1080

gucgagauca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc     1140

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1320

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1380

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc     1440

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1500

cucugcaccg ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc     1560

accgucgagg uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc     1620

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1680

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1740

aucgucaucg gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc     1800

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1860

accgccuggg acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1980

cucaucggca cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc     2040

augugccucg cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8079
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8079
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccga ggucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8080
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8080
gggagaaagc uuaccauggg cgccgacacc uccgucggca ucgucggccu ccuccucacc       60

accgccaugg ccgccgaggu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgccg gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgccgccg ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag     1080

aucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc     2040

cucgcccucg gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8081
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8081
gggagaaagc uuaccauggc cgacaccucc gucggcaucg ucggccuccu ccucaccacc       60

gccauggccg ccgaggucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  8082
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8082
gggagaaagc uuaccaugga caccuccguc ggcaucgucg gccuccuccu caccaccgcc       60

auggccgccg aggucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg ccugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8083
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  8083
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8084
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8084
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8085
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8085
gggagaaagc uuaccaugcg cggcgccgac accuccgucg gcaucgucgg ccuccuccuc       60

accaccgcca uggccgccgc cgucacccgc cgcggcuccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaggc caucuccuuc cccaccaccc ucggcaugaa caagugcuac      180

auccagauca uggaccucgg ccacaugugc gacgccacca uguccuacga gugccccaug      240

cucgacgagg gcgucgagcc cgacgacguc gacugcuggu gcaacaccac cuccaccugg      300

gucgucuacg gcaccugcca ccacaagaag ggcgaggccc gccgcucccg ccgcgccguc      360

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      420

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      480

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      540

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      600

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      660

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      720

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      780

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      840

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      900

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      960

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag     1020

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc     1080

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc     1140

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac     1200

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1320

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1380

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1440

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1560

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1620

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1680

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1740

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1800

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1860

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1980

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     2040

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8086
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8086
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcggcgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8087
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8087
gggagaaagc uuaccaugcg cggccucucc ucccgcaagc gccgcuccca cgacguccuc       60

accguccagu uccucauccu cggcaugcuc cucaugaccg gcgccgucac ccgccgcggc      120

uccgccuacu acauguaccu cgaccgcaac gacgccggcg aggccaucuc cuuccccacc      180

acccucggca ugaacaagug cuacauccag aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgagugccc caugcucgac gagggcgucg agcccgacga cgucgacugc      300

uggugcaaca ccaccuccac cugggucguc uacggcaccu gccaccacaa gaagggcgag      360

gcccgccgcu cccgccgcgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc      420

cgcucccaga ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac      480

uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc      540

uccuccaccu cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac      600

uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc      660

ugggucgaca ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc      720

accgucgaca ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac      780

ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag      840

gccuaccucg acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc      960

gccugcucca agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc     1020

augcucuccg uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag     1080

accgacgaga accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc     1140

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc     1200

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac     1260

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1380

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1440

gccaagggcc gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1500

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1620

aagguccccg cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1680

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1740

ccccccuucg gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac     1800

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1860

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac     1920

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1980

auguccuggu ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc     2040

aagaacggcu ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc     2100

accgccgucu ccgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8088
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 3 opt

<400>  8088
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8089
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 3 opt

<400>  8089
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacacc      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacgcca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8090
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 3 opt

<400>  8090
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc     1080

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     2040

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcuccguc gacuucucca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8091
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 3 opt

<400>  8091
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8092
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 3 opt

<400>  8092
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8093
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 3 opt

<400>  8093
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucacccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8094
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 3 opt

<400>  8094
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccgcaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8095
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 3 opt

<400>  8095
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaccgu caacgacauc     1080

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8096
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 3 opt

<400>  8096
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc     1080

gacgagaacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1140

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1200

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1260

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1380

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1440

aagggcaagc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1500

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1560

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1620

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1680

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1740

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1800

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1860

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1920

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1980

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     2040

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     2100

gccgucuccg ccgacgucgg cugcuccguc gacuucucca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8097
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 3 opt

<400>  8097
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggccg cggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8098
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 3 opt

<400>  8098
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacgccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8099
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 3 opt

<400>  8099
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc cucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8100
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 3 opt

<400>  8100
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu gguaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8101
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 3 opt

<400>  8101
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagauc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8102
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 3 opt

<400>  8102
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccucgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8103
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 3 opt

<400>  8103
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc acccucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8104
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 3 opt

<400>  8104
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgucgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8105
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 3 opt

<400>  8105
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccgcc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcccccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8106
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 3 opt

<400>  8106
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc caagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8107
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 3 opt

<400>  8107
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccacccg ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8108
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 3 opt

<400>  8108
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcuccgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaccc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8109
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 3 opt

<400>  8109
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccaacg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgcccgcc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8110
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 3 opt

<400>  8110
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gagaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8111
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 3 opt

<400>  8111
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu cccuccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8112
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 3 opt

<400>  8112
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccaacg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggauguuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8113
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 3 opt

<400>  8113
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggauccuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcaucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8114
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 3 opt

<400>  8114
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc uccaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc caccaucucc gacaucgccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgccguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8115
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 3 opt

<400>  8115
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggca cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8116
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 3 opt

<400>  8116
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagc uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8117
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 3 opt

<400>  8117
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gagaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

aucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8118
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 3 opt

<400>  8118
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaucg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8119
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 3 opt

<400>  8119
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ucaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucuccuuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8120
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 3 opt

<400>  8120
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggcaa gcucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8121
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 3 opt

<400>  8121
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gaggcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8122
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 3 opt

<400>  8122
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcgccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8123
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 3 opt

<400>  8123
gggagaaagc uuaccaugcg caucaucaac gccggcaagg agaagaagcg ccgcggcgcc       60

gacaccaacg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgcccgcc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8124
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 3 opt

<400>  8124
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gagaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaagg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8125
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 3 opt

<400>  8125
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggucu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8126
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 3 opt

<400>  8126
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccaacg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacau gaccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8127
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 3 opt

<400>  8127
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccgccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagacc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8128
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 3 opt

<400>  8128
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gagaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cucgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8129
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 3 opt

<400>  8129
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcauc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcauccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8130
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 3 opt

<400>  8130
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacacc      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cauccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacgcca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8131
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 3 opt

<400>  8131
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccagcgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8132
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 3 opt

<400>  8132
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccaggccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8133
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 3 opt

<400>  8133
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8134
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 3 opt

<400>  8134
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac accggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gagggcaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

guccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8135
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 3 opt

<400>  8135
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgc cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugcucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgc ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg ccaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agaccgucga cggcaccguc accgucgagg gccaguacgg cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucaucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cggcggcguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8136
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 3 opt

<400>  8136
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucuccaag     1560

auccccgccg agacccucca cggcaccguc accgaggagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8137
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 3 opt

<400>  8137
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8138
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 3 opt

<400>  8138
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggca acgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca ccaugggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8139
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 3 opt

<400>  8139
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     2040

cucaacacca agaacggcuc caucucccuc gucugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8140
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 3 opt

<400>  8140
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcacc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagcccg ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8141
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 3 opt

<400>  8141
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgu caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8142
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 3 opt

<400>  8142
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcga ggccaucucc      180

uuccccacca cccucggcgu caacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu gguaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8143
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 3 opt

<400>  8143
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agaagaagcg ccgcggcgcc       60

gacaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgu cgaggucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uuccccacca cccucggcgu caacaagugc uacauccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cguccucauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8144
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 3 opt

<400>  8144
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucacccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8145
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 3 opt

<400>  8145
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca accucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcguccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8146
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 3 opt

<400>  8146
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8147
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 3 opt

<400>  8147
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca accucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8148
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 3 opt

<400>  8148
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8149
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 3 opt

<400>  8149
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccagcacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgucugcac cgccgccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8150
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 3 opt

<400>  8150
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacgccucca ucggcaucgu cucccuccuc cucaccaccg ucauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgucacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acuccggcgc cgacaccgag accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggccgcg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ucaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8151
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 3 opt

<400>  8151
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      540

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag     1080

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc     1140

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac     1200

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc     1260

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1320

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1380

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1440

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1500

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1560

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1620

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1680

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1740

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1800

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1860

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgucuucaa cucccucggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1980

uucucccaga uccucaucgg cacccuccuc gucuggcucg gccucaacac caagaacggc     2040

uccaucuccc ucaccugccu cgcccucggc ggcgucauga ucuuccucuc caccgccguc     2100

uccgccgacg ucggcugcuc cgucgacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8152
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 3 opt

<400>  8152
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgaga cccgccgcuc ccgccgcucc gucucccucc gcuaccacua cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaagaagca ccucaucaug      480

gucgagaacu ggaucuuccg caaccccggc uucgccaucg ucuccgucgc caucaccugg      540

cucaugggcu cccucaccuc ccagaagguc aucuaccucg ucaugaucgu ccucaucguc      600

cccgccuacu ccaucuccug caucggcguc uccaaccgcg accucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccga gauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaugaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cucccucucc gacauggccu ccgccucccg cugccccacc      840

cagggcgagc ccucccucga caagcagucc gacacccagu ccgucugcaa gcgcacccuc      900

ggcgaccgcg gcuggggcaa cggcugcggc aucuucggca agggcucccu cgucaccugc      960

uccaaguuca ccugcugcaa gaagaugccc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcuccccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguccgc cggcaccgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc accugccucg cccucggcgg cgucaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8153
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 3 opt

<400>  8153
gggagaaagc uuaccaugcg caucaucaac gcccgcaagg agcgcaagcg ccgcggcgcc       60

gacaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgagaucacc      120

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      180

uucgccacca ccaugggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      240

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      360

aagggcgagg cccgccccuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      420

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      480

gucgagaacu ggauccuccg caaccccggc uucaccgucg ccgcccucgc cauggccugg      540

cuccucggca acuccaccuc ccagcgcguc aucuaccucg ucaugauccu ccucaucgcc      600

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      660

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      720

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      780

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      840

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      960

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag     1020

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc     1080

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc     1140

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1200

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1260

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1380

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1440

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgcau caagauggac     1500

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucuccaag     1560

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggccgcgac     1620

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1680

cgccucauca ccgccaaccc caucaucacc gaguccaccg agaacucccg caugaugcuc     1740

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1800

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1860

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1920

gucuucaacu cccucggcaa gggcguccac caggucuucg gcgccgccuu caagucccuc     1980

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucgu cuggcucggc     2040

cucaacacca agaacggcuc caucucccuc gccugccucg cccucggcgg cgccaugauc     2100

uuccucucca ccgccgucuc cgccgacguc ggcugcuccg ucgacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  8154
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 3 opt

<400>  8154
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8155
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 3 opt

<400>  8155
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8156
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 3 opt

<400>  8156
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8157
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 3 opt

<400>  8157
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8158
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 3 opt

<400>  8158
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcua cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggcaagcucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagauc cccguccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8159
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 3 opt

<400>  8159
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc gcaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcua cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggcaagcucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagauc cccguccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8160
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 3 opt

<400>  8160
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

accgucaacg acaucggcua cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggcaagcucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagauc cccguccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8161
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 3 opt

<400>  8161
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucac ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8162
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 3 opt

<400>  8162
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga gaaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg caagcucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8163
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 3 opt

<400>  8163
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggccgcggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8164
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 3 opt

<400>  8164
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggccucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8165
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 3 opt

<400>  8165
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacugguac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8166
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 3 opt

<400>  8166
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga cacccagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8167
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 3 opt

<400>  8167
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agauccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8168
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 3 opt

<400>  8168
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cucgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8169
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 3 opt

<400>  8169
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccacccu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8170
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 3 opt

<400>  8170
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccguc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8171
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 3 opt

<400>  8171
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccgccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcccc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8172
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 3 opt

<400>  8172
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accuccaaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8173
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 3 opt

<400>  8173
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc acccgccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8174
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 3 opt

<400>  8174
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcacccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8175
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 3 opt

<400>  8175
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacucccuc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8176
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 3 opt

<400>  8176
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcaucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8177
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 3 opt

<400>  8177
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc cuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcauc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8178
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 3 opt

<400>  8178
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucuccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccacca ucuccgacau cgccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ccgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8179
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 3 opt

<400>  8179
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagcuccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8180
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 3 opt

<400>  8180
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caucgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8181
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 3 opt

<400>  8181
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgucaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu ccuucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8182
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 3 opt

<400>  8182
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggcaagcucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8183
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 3 opt

<400>  8183
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caaggacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8184
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 3 opt

<400>  8184
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggaggc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8185
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 3 opt

<400>  8185
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cgcccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agacccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8186
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 3 opt

<400>  8186
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggcg ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8187
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 3 opt

<400>  8187
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccucgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8188
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 3 opt

<400>  8188
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggucuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8189
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 3 opt

<400>  8189
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcaucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggca uccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8190
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 3 opt

<400>  8190
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaucc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8191
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 3 opt

<400>  8191
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccag cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8192
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 3 opt

<400>  8192
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca ggcccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8193
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 3 opt

<400>  8193
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuucu ccaagauccc cgccgagacc cuccacggca ccgucaccga ggagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8194
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 3 opt

<400>  8194
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacaccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaggg caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcguccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8195
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 3 opt

<400>  8195
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8196
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 3 opt

<400>  8196
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcaacgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccaccaug ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8197
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 3 opt

<400>  8197
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucgucug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8198
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 3 opt

<400>  8198
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucaacaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucgucug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8199
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 3 opt

<400>  8199
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccgccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8200
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 3 opt

<400>  8200
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgucaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8201
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 3 opt

<400>  8201
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcgucaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacugguac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8202
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 3 opt

<400>  8202
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgucgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuuccc caccacccuc ggcgucaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8203
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 3 opt

<400>  8203
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucac ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8204
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 3 opt

<400>  8204
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccaaccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggcg uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8205
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 3 opt

<400>  8205
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8206
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 3 opt

<400>  8206
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccaaccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8207
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 3 opt

<400>  8207
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8208
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 3 opt

<400>  8208
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccagc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccguc     1500

ugcaccgccg ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8209
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 3 opt

<400>  8209
gggagaaagc uuaccaugac cuccaucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acgagaaccg cgccaagguc gaggucaccc ccaacucccc ccgcgccgag     1080

gccacccucg gcggcuucgg cucccucggc cucgacugcg agccccgcac cggccucgac     1140

uucuccgacc ucuacuaccu caccaugaac aacaagcacu ggcucgucca caaggagugg     1200

uuccacgaca ucccccuccc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccucgucga guucaaggac gcccacgcca agcgccagac cgucgucguc     1320

cucggcuccc aggagggcgc cguccacacc gcccucgccg gcgcccucga ggccgagaug     1380

gacggcgcca agggccgccu cuucuccggc caccucaagu gccgccucaa gauggacaag     1440

cuccgccuca agggcgucuc cuacucccuc ugcaccgccg ccuucaccuu caccaagguc     1500

cccgccgaga cccuccacgg caccgucacc gucgaggucc aguacgccgg caccgacggc     1560

cccugcaagg uccccgccca gauggccguc gacaugcaga cccucacccc cgucggccgc     1620

cucaucaccg ccaaccccgu caucaccgag uccaccgaga acuccaagau gaugcucgag     1680

cucgaccccc ccuucggcga cuccuacauc gucaucggcg ucggcgacaa gaagaucacc     1740

caccacuggc accgcuccgg cuccaccauc ggcaaggccu ucgaggccac cguccgcggc     1800

gccaagcgca uggccguccu cggcgacacc gccugggacu ucggcuccgu cggcggcguc     1860

uucaacuccc ucggcaaggg cauccaccag aucuucggcg ccgccuucaa gucccucuuc     1920

ggcggcaugu ccugguucuc ccagauccuc aucggcaccc uccucgucug gcucggccuc     1980

aacaccaaga acggcuccau cucccucacc ugccucgccc ucggcggcgu caugaucuuc     2040

cucuccaccg ccgucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8210
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 3 opt

<400>  8210
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccgaga ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgagacccgc cgcucccgcc gcuccgucuc ccuccgcuac      360

cacuacaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacaag      420

aagcaccuca ucauggucga gaacuggauc uuccgcaacc ccggcuucgc caucgucucc      480

gucgccauca ccuggcucau gggcucccuc accucccaga aggucaucua ccucgucaug      540

aucguccuca ucguccccgc cuacuccauc uccugcaucg gcgucuccaa ccgcgaccuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgagaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccau gaccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccuccc ucuccgacau ggccuccgcc      780

ucccgcugcc ccacccaggg cgagcccucc cucgacaagc aguccgacac ccaguccguc      840

ugcaagcgca cccucggcga ccgcggcugg ggcaacggcu gcggcaucuu cggcaagggc      900

ucccucguca ccugcuccaa guucaccugc ugcaagaaga ugcccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc cccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaucggcca cgagaccgac gagaaccgcg ccaaggucga ggucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uccgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     2040

ggcggcguca ugaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8211
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 3 opt

<400>  8211
gggagaaagc uuaccauguc cgucggcauc gucggccucc uccucaccac cgccauggcc       60

gccgagguca cccgccgcgg cuccgccuac uacauguacc ucgaccgcaa cgacgccggc      120

gaggccaucu ccuuccccac cacccucggc augaacaagu gcuacaucca gaucauggac      180

cucggccaca ugugcgacgc caccaugucc uacgagugcc ccaugcucga cgagggcguc      240

gagcccgacg acgucgacug cuggugcaac accaccucca ccugggucgu cuacggcacc      300

ugccaccaca agaagggcga ggcccgccgc ucccgccgcg ccgucacccu ccccucccac      360

uccacccgca agcuccagac ccgcucccag accuggcucg agucccgcga guacaccaag      420

caccucaucc gcgucgagaa cuggaucuuc cgcaaccccg gcuucgcccu cgccgccgcc      480

gccaucgccu ggcuccucgg cuccuccacc ucccagaagg ucaucuaccu cgucaugauc      540

cuccucaucg cccccgccua cuccauccgc ugcaucggcg ucuccaaccg cgacuucguc      600

gagggcaugu ccggcggcac cugggucgac gucguccucg agcacggcgg cugcgucacc      660

gucauggccc aggacaagcc caccgucgac aucgagcucg ucaccaccac cgucuccaac      720

auggccgagg uccgcuccua cugcuacgag gccuccaucu ccgacauggc cuccgacucc      780

cgcugcccca cccagggcga ggccuaccuc gacaagcagu ccgacaccca guacgucugc      840

aagcgcaccc ucgucgaccg cggcuggggc aacggcugcg gccucuucgg caagggcucc      900

cucgucaccu gcgccaaguu cgccugcucc aagaagauga ccggcaaguc cauccagccc      960

gagaaccucg aguaccgcau caugcucucc guccacggcu cccagcacuc cggcaugauc     1020

gucaacgaca ccggccacga gaccgacgag aaccgcgcca aggucgagau cacccccaac     1080

uccccccgcg ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc     1140

cgcaccggcc ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc     1200

guccacaagg agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc     1320

cagaccgucg ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc     1380

cucgaggccg agauggacgg cgccaagggc cgccucuccu ccggccaccu caagugccgc     1440

cucaagaugg acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac     1560

gccggcaccg acggccccug caaggucccc gcccagaugg ccgucgacau gcagacccuc     1620

acccccgucg gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc     1680

aagaugaugc ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc     1740

gagaagaaga ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag     1800

gccaccgucc gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc     1860

uccgucggcg gcgcccucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaaguccc ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc     1980

auguggcucg gccucaacac caagaacggc uccaucuccc ucaugugccu cgcccucggc     2040

ggcguccuca ucuuccucuc caccgccguc uccgccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  8212
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 3 opt

<400>  8212
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc ccgccgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8213
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 3 opt

<400>  8213
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggaug uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8214
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 3 opt

<400>  8214
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaugaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8215
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 3 opt

<400>  8215
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccgagg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgccaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

cucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucgcccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgccacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc gucgacggca ccgucaccgu cgagggccag     1560

uacggcggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau caucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8216
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 3 opt

<400>  8216
gggagaaagc uuaccauggg cgccgacgcc uccaucggca ucgucucccu ccuccucacc       60

accgucaugg ccgccgagau cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

uccgacgccg gcaaggccau cuccuucguc accacccucg gcgucaacaa gugccacguc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau caaggucgag aacuggaucu uccgcaaccc cggcuucgcc      480

cucgucgccg ucgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucaccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga cgagaaccgc gccaaggucg aggucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacuccg gcgccgacac cgagaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uccuccggcc accucaagug ccgccucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

accaaggucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

cgcgacggcc ccugcaaggu ccccgcccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgacaag     1740

aagaucaccc accacuggca ccgcuccggc uccaucaucg gcaaggccuu cgaggccacc     1800

guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc     1860

ggcggcgucu ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

ucccucuucg gcggcauguc cugguucucc cagauccuca ucggcacccu ccucgucugg     1980

cucggccuca acaccaagaa cggcuccauc ucccucaccu gccucgcccu cggcggcguc     2040

augaucuucc ucuccaccgc cgucuccgcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  8217
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 3 opt

<400>  8217
gggagaaagc uuaccauggg cgccgacacc uccaucggca ucaucggccu ccuccucacc       60

accgccaugg ccgccgagau cacccgccgc ggcuccgccu acuacaugua ccucgaccgc      120

uccgacgccg gcaaggccau cuccuucgcc accaccaugg gcgucaacaa gugccacguc      180

cagaucaugg accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc      240

gacgagggcg ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc      300

gucuacggca ccugccacca caagaagggc gaggcccgcc ccucccgccg cgccgucacc      360

cuccccuccc acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc      420

gaguacacca agcaccucau caaggucgag aacuggaucc uccgcaaccc cggcuucacc      480

gucgccgccc ucgccauggc cuggcuccuc ggcaacucca ccucccagcg cgucaucuac      540

cucgucauga uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac      600

cgcgacuucg ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc      660

ggcugcguca ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc      720

accgucucca acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug      780

gccuccgacu cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc      840

caguacgucu gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc      900

ggcaagggcu cccucgucac cugcgccaag uucaccugcu ccaagaagau gaccggcaag      960

uccauccagc ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac     1020

uccggcauga ucgucaacga caucggccac gagaccgacg agaaccgcgc caaggucgag     1080

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac     1440

cucaagugcc gcaucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucuc caaggucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggccg cgacggcccc ugcaaggucc ccgcccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccaucau caccgagucc     1680

accgagaacu cccgcaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgucuuc aacucccucg gcaagggcgu ccaccagguc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucgucuggcu cggccucaac accaagaacg gcuccaucuc ccucgccugc     2040

cucgcccucg gcggcgccau gaucuuccuc uccaccgccg ucuccgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8218
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 3 opt

<400>  8218
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8219
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 3 opt

<400>  8219
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8220
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 3 opt

<400>  8220
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc      540

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc      600

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      660

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      720

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc      840

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc      900

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc      960

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1020

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1140

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1200

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1260

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1320

auggccgucc ucggcgacac cgccugggac uucggcuccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8221
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 3 opt

<400>  8221
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8222
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 3 opt

<400>  8222
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8223
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 3 opt

<400>  8223
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaccgu caacgacauc      540

ggcuacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggcaa gcucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agauccccgu ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8224
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 3 opt

<400>  8224
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuucu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc uccugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  8225
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 3 opt

<400>  8225
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc      540

gacgagaacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc      600

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      660

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      720

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc      840

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc      900

aagggcaagc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc      960

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1020

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1080

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1140

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1200

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1260

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1320

auggccgucc ucggcgacac cgccugggac uucggcuccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8226
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 3 opt

<400>  8226
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu cccuccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8227
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 3 opt

<400>  8227
gggagaaagc uuaccauguc caagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8228
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 3 opt

<400>  8228
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu gguaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8229
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 3 opt

<400>  8229
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggcaa gcucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8230
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 3 opt

<400>  8230
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaccc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8231
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 3 opt

<400>  8231
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagauc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8232
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 3 opt

<400>  8232
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgcccgcc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8233
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 3 opt

<400>  8233
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggucu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8234
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 3 opt

<400>  8234
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaucg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8235
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 3 opt

<400>  8235
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcaucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8236
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 3 opt

<400>  8236
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cucgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8237
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 3 opt

<400>  8237
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggca cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8238
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 3 opt

<400>  8238
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccucgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8239
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 3 opt

<400>  8239
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gaggcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8240
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 3 opt

<400>  8240
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccgccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8241
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 3 opt

<400>  8241
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc uccaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc caccaucucc gacaucgccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgccguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8242
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 3 opt

<400>  8242
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucuccaag     1020

auccccgccg agacccucca cggcaccguc accgaggagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8243
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 3 opt

<400>  8243
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagc uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8244
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 3 opt

<400>  8244
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcauc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcauccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8245
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 3 opt

<400>  8245
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ucaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8246
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 3 opt

<400>  8246
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcgccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8247
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 3 opt

<400>  8247
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac accggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gagggcaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8248
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 3 opt

<400>  8248
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgc cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugcucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgc ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg ccaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agaccgucga cggcaccguc accgucgagg gccaguacgg cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8249
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 3 opt

<400>  8249
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagcccg ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8250
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 3 opt

<400>  8250
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc aacaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8251
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 3 opt

<400>  8251
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcgccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8252
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 3 opt

<400>  8252
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8253
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 3 opt

<400>  8253
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu gguaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8254
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 3 opt

<400>  8254
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8255
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 3 opt

<400>  8255
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8256
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 3 opt

<400>  8256
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgucugcac cgccgccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8257
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 3 opt

<400>  8257
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacgag      540

aaccgcgcca aggucgaggu cacccccaac uccccccgcg ccgaggccac ccucggcggc      600

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      660

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      720

cuccccuggc acuccggcgc cgacaccgag accccccacu ggaacaacaa ggaggcccuc      780

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag      840

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc      900

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc      960

gucuccuacu cccucugcac cgccgccuuc accuucacca agguccccgc cgagacccuc     1020

cacggcaccg ucaccgucga gguccaguac gccggccgcg acggccccug caaggucccc     1080

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1140

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1200

ggcgacuccu acaucgucau cggcgucggc gacaagaaga ucacccacca cuggcaccgc     1260

uccggcucca ucaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1320

guccucggcg acaccgccug ggacuucggc uccugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  8258
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 3 opt

<400>  8258
gggagaaagc uuaccauguc ccagaagguc aucuaccucg ucaugaucgu ccucaucguc       60

cccgccuacu ccaucuccug caucggcguc uccaaccgcg accucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccga gauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaugaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cucccucucc gacauggccu ccgccucccg cugccccacc      300

cagggcgagc ccucccucga caagcagucc gacacccagu ccgucugcaa gcgcacccuc      360

ggcgaccgcg gcuggggcaa cggcugcggc aucuucggca agggcucccu cgucaccugc      420

uccaaguuca ccugcugcaa gaagaugccc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcuccccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguccgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8259
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 3 opt

<400>  8259
gggagaaagc uuaccaugcg ccagaagguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8260
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 3 opt

<400>  8260
gggagaaagc uuaccauguc ccagcgcguc aucuaccucg ucaugauccu ccucaucgcc       60

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      120

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      180

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      240

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      300

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      360

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      420

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      480

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacauc      540

ggccacgaga ccgacgagaa ccgcgccaag gucgagguca cccccaacuc cccccgcgcc      600

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc      840

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag      900

auggacggcg ccaagggccg ccucuucucc ggccaccuca agugccgcau caagauggac      960

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucuccaag     1020

guccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggccgcgac     1080

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1140

cgccucauca ccgccaaccc caucaucacc gaguccaccg agaacucccg caugaugcuc     1200

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga caagaagauc     1260

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1320

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8261
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  8261
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8262
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 3 opt

<400>  8262
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacacu      240

ugugacgcca ccaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac aaccaguacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacccuac cgucccauuc uacgaggaag      420

cuccaaacuc ggucgcagac cuggcuggag ucacgcgaau acacaaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacuuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcacag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccacc      840

caaggagaag ccuaccuuga caaacagagc gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugaccugc      960

gcaaaguuug cuugcucuaa gaaaaugacg gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cagacgagaa ucgagccaaa gucgaaauca cuccaaacuc accccgcgcu     1140

gaggccaccc uugggggauu cggcucccug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagug aucuguauua cuuaacuaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auaccggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau acugcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacgg ccgcauucac auuuaccaaa     1560

auaccugcgg aaacucucca uggcaccgug acaguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacucugac accugugggc     1680

agguuaauca cggccaaccc aguuauuacc gaaaguacug aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acacaccacu ggcaucguuc uggaucaacc aucgguaagg cuuuugaggc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acugccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaaugcaa agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa cggcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8263
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 3 opt

<400>  8263
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugg cuacgaaacc     1080

gaugaggacc gagcaaaggu cgaggugaca ccuaauucac cacgcgcuga agccacucuu     1140

gggggauucg gcagucuggg uuuggauugu gagcccagaa ccgggcucga cuuuagcgau     1200

cuguauuacu uaacaaugaa caauaaacac uggcugguac auaaggaaug guuccacgac     1260

auaccucucc cauggcacgc uggcgccgau acuggaaccc cgcauuggaa caauaaagag     1320

gcacuuguug aauuuaagga cgcccacgcu aagaggcaga caguggucgu gcugggcucc     1380

caggaggggg cgguccauac cgcauuggcc ggagcucuag aggccgaaau ggauggugca     1440

aaaggccggc uguucucugg gcaccucaag ugccgccuga aaauggacaa acuuagacug     1500

aagggaguga gcuauucauu guguacugcc gcuuuuacgu ucacaaaggu ucccgccgag     1560

acccuccaug gcacugugac cguagaaguc caguacgcag guacagacgg gccuugcaag     1620

aucccagugc aaauggcggu ugauaugcag acccugacuc ccgugggcag guuaauuaca     1680

gcuaacccug ucaucacgga gaguaccgaa aauuccaaaa ugaugcugga gcuagaccca     1740

cccuuuggag auagcuacau agugauuggc guaggggaca agaaaaucac ucaccacugg     1800

caucguucug gaucaacaau cgguaaggcc uucgaggcaa ccgugcgggg cgccaaacga     1860

auggcuguuc ucggggauac agccugggac uucggaagug ucggcggugu guuuaacagc     1920

cuggggaagg gcauucacca gaucuucgga gcagcuuuua agucccuuuu cggcgggaug     1980

ucuugguuuu cgcaaauuuu gauaggaacu cugcucgugu ggcugggccu uaacaccaaa     2040

aaugguagca ucucccugac guguuuggcc cucgggggag ucaugauuuu ccugucaacc     2100

gcgguuagug ccgauguggg cugcucugug gacuuuagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8264
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 3 opt

<400>  8264
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8265
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  8265
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8266
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 3 opt

<400>  8266
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cgccaagaau ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaacggca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8267
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 3 opt

<400>  8267
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgguuacg agaccgacga agaucgugcg aaggucgagg ugacaccuaa cucaccacgc     1080

gccgaggcaa cucuuggcgg guucggaagu cugggcuugg acugugaacc cagaaccggu     1140

cucgauuuua gcgaccugua uuacuuaaca augaauaaca aacacuggcu gguacauaag     1200

gagugguucc acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau     1260

uggaauaaca aagaagcucu uguugaguuu aaggaugccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucccagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggacg gagcuaaggg ucggcuguuc ucuggccacc ucaagugccg acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucauugugua cugccgcauu uacguucaca     1500

aagguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gacgguccuu gcaaaauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggagauuaa uaacagcaaa uccugucauc acggaaagua ccgagaacuc caagaugaug     1680

cuggaacuag auccacccuu uggcgacagc uacauuguga ucggaguagg cgauaagaaa     1740

auaacucacc acuggcauag gucuggguca acaauuggaa aggcguucga ggcuaccgug     1800

cggggugcca aacgcauggc aguucucggc gacacagccu gggauuucgg gagugucgga     1860

ggcguguuua auagccuggg uaaggggauc caccagaucu ucggcgcugc cuuuaaaucc     1920

cuuuucggag gcaugucuug guuuucgcaa auuuugaucg ggacucugcu cguguggcug     1980

ggacuuaaca ccaagaacgg cagcauuucc cugacguguu uggcacucgg uggggucaug     2040

auauuccugu caaccgcugu uagugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8268
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 3 opt

<400>  8268
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8269
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 3 opt

<400>  8269
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagauau cgugcuggaa cacggcgggu gugucacagu gauggcacaa      180

gacaaaccua cuguugauau ugaguuggug acgaccacag ucaguaauau ggccgaagug      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag guggaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ccauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ugaguucaag gaugcacacg ccaagagaca gacaguggua      840

guguugggcu cccaggaagg ggcuguccau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auccccgcug agacccugca cggcacuguu acaguggaag uccaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacguuaac cccgguuggc     1140

cgacugauaa cagcaaaucc cgugauuacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgucauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8270
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 3 opt

<400>  8270
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8271
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 3 opt

<400>  8271
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgg cuaugaaacc      540

gaugaggaca gggccaaggu ggaagucacg cccaacaguc cucgggcuga ggcaacucug      600

ggaggguuug gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau      660

cuguacuacc ucacaaugaa uaacaaacau ugguuaguuc acaaggaaug guuucaugac      720

auuccccugc cuuggcacgc cggagcugac acuggcaccc cacacuggaa uaacaaagag      780

gcccuugugg aguucaagga ugcgcaugca aagagacaga caguaguggu cuugggcucc      840

caggaagggg ccgugcacac cgcucuggcc ggagcacuag aagcugagau ggacggugcc      900

aaaggcaggc ucuuuucagg gcaucugaag ugucggcuua aaauggauaa gcugcgcuug      960

aaaggaguua gcuauagucu cugcacggcg gccuucacuu uuacaaaggu gcccgcagag     1020

acccugcacg gcacugucac aguggaagua caauacgcug guaccgacgg gccuuguaaa     1080

aucccaguuc agauggccgu ggauaugcag acguuaaccc cggucggccg acugauuaca     1140

gcuaaucccg ugaucacuga gucuaccgaa aacuccaaga ugaugcucga gcuugacccu     1200

ccauucggag auagcuauau agucauuggc gugggggaca agaaaaucac acaccauugg     1260

cacagaucgg gaucuacuau ugguaaggcc uuugaagcaa ccguuagggg cgccaaacgg     1320

auggcugugc ugggggauac agcgugggac uucggauccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8272
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 3 opt

<400>  8272
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8273
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       3 opt

<400>  8273
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcugag uggaaagggc      900

ucucuaguca caugcgcgaa auucgccugc uccaagaaaa ugaccggcaa gagcauucag      960

ccugaaaacc ucgaguaccg uaucaugcug ucggugcacg ggucucaaca cuccggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauucaccca gagcugaagc cacucuuggc ggguuuggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu cagcgaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ucacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggagu ucaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaucccag gaaggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gagacugaaa ggggugucau auaguuugug uacugccgcu     1500

uuuacguuca caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uuuggcgacu cuuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucaggga guacaauugg aaaggcauuc     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuu     1860

gggagcguag gaggcgcucu uaauucccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguucaaau cuuuguuugg aggcaugucg ugguucagcc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcuccauuu cauugaugug ucucgcccug     2040

gguggggugu uaauauuucu gaguacggcu guuucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8274
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 3
       opt

<400>  8274
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcugag uggaaagggc      900

ucucuaguga ccugcgcgaa auucgccugc uccaagaaaa ugacgggcaa gagcauacag      960

ccugaaaacc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauucaccca gagcugaagc cacccuuggc ggguuuggaa gucugggcuu ggacugugag     1140

ccuaggacag gucucgauuu cagcgaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ucacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagagu ucaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaucccag gaaggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gagacugaaa ggggucucau auaguuugug uacggccgcu     1500

uuuacauuca ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgaguc cacugaaaac     1680

agcaagauga ugcuggagcu agaucccccg uuuggcgacu cuuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucaggga guaccauagg aaaggcauuc     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuu     1860

gggagcgugg gaggcgcucu uaauucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguucaaau cuuuguuugg aggcaugucg ugguucagcc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cgccaagaau ggcuccaucu cauugaugug ucucgcucug     2040

ggugggguuu uaauuuuucu gaguacggca gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8275
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 3 
       opt

<400>  8275
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguc uggaaagggc      900

ucccuaguua caugcgccaa auuuaccugc agcaagaaga ugacgggcaa aucgauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucugugcacg ggucccaaca uucaggaaug     1020

aucgguuacg agaccgacga agaucgugcg aaggucgagg ugacaccuaa caguccacgc     1080

gccgaggcaa cucuuggcgg guucggaagc cugggcuugg acugugaacc cagaaccggu     1140

cucgauuucu ccgaccugua uuacuuaaca augaauaaca aacacuggcu gguacauaag     1200

gagugguuuc acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau     1260

uggaauaaca aagaagcucu uguugaguuc aaggaugccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucucagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggacg gagcuaaggg ucggcuguuu agcggccacc ucaagugccg acugaaaaug     1440

gauaaacuuc gccugaaggg ggugucauau aguuugugua cugccgcauu cacguuuaca     1500

aagguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gacgguccuu gcaaaauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggagauuaa uaacagcaaa uccugucauc acggaaucca ccgagaacag caagaugaug     1680

cuggaacuag auccacccuu cggcgacucu uacauuguga ucggaguagg cgauaagaaa     1740

auaacucacc acuggcauag gucagggagu acaauuggaa aggcguuuga ggcuaccgug     1800

cggggugcca aacgcauggc aguucucggc gacacagccu gggauuucgg gagcgucgga     1860

ggcguguuca auucccuggg uaaggggauc caccagaucu uuggcgcugc cuucaaaucu     1920

cuuuuuggag gcaugucgug guucagccaa auuuugaucg ggacucugcu cguguggcug     1980

ggacuuaaca ccaagaacgg cuccauuuca cugacguguu uggcacucgg uggggucaug     2040

auauuucuga guaccgcugu uucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8276
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F399S) mRNA Design 3 
       opt

<400>  8276
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcugag uggaaagggc      900

ucucuaguga caugcgcgaa auucgccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaacc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauucaccca gagcugaagc cacucuuggc ggguuuggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu cagcgaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ucacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagagu ucaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaucccag gaaggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gagacugaaa ggggucucau auaguuugug uacugccgcu     1500

uuuacguuca caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uuuggcgacu cuuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucaggga guacaauagg aaaggcauuc     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuu     1860

gggagcgugg gaggcgcucu uaauucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguucaaau cuuuguuugg aggcaugucg ugguucagcc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcuccaucu cauugaugug ucucgcccug     2040

ggugggguuu uaauuuuucu gaguacggcu gucucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8277
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       3 opt

<400>  8277
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguucagg acacggguca ugaaaccgau gagaaucgcg caaaggugga gaucacacca     1080

aacuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaauaa caaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucaa     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaga uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aauccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaacagccuu gguaagggga uccaccaaau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc agauucugau cgggacucuc     1980

cugauguggc uuggacugaa uaccaagaac ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8278
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 3
       opt

<400>  8278
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugcagg acaccgguca ugaaacagau gagaaucgcg caaaggucga gauuacucca     1080

aacuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcaa     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaga uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aauccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaacagccuu gguaagggga uucaccaaau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc agauccugau ugggacccuc     1980

cugauguggc uuggacugaa ugccaagaac ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaacggca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8279
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 3 
       opt

<400>  8279
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugcagg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcaa     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaga uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aauccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaacagccuu gguaagggga uucaccaaau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc agauccugau ugggacucuc     1980

cugauguggc uuggacugaa uaccaagaac ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8280
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8280
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacacucgg aaaggcauuu     1800

aguaccacac ugaaaggugc ccagcgccuu gcugccuugg gcgauacugc augggacuuc     1860

gggagcauug gaggcgucuu caauuccauc gguagagcug ugcaccaagu auuugggggc     1920

gccuucagga cccuguuugg aggcaugucu uggaucacgc aggggcucau gggagcgcug     1980

cuucugugga ugggcgugaa cgcccgugau cggucgauug cuuuggcauu ccucgccacc     2040

ggugggguuc uggucuuuuu agcuacaaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8281
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8281
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caacccucgg aaaggcauuu     1800

aguacaacuc ugaaaggugc ccagcgccuu gcugccuugg gcgauaccgc augggacuuc     1860

gggagcauag gaggcguguu caauuccauu gguagagcug uacaccaagu guuugggggc     1920

gccuucagga cgcuguuugg aggcaugucu uggaucaccc aggggcucau gggagcgcug     1980

cuucugugga ugggcguuaa cgcccgugau cggucgaucg cuuuggcauu ccucgccaca     2040

gguggggucc ugguguuuuu agcuacuaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8282
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 3 opt

<400>  8282
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgguuacg agaccgacga agaucgugcg aaggucgagg ugacaccuaa cucaccacgc     1080

gccgaggcaa cucuuggcgg guucggaagu cugggcuugg acugugaacc cagaaccggu     1140

cucgauuuua gcgaccugua uuacuuaaca augaauaaca aacacuggcu gguacauaag     1200

gagugguucc acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau     1260

uggaauaaca aagaagcucu uguugaguuu aaggaugccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucccagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggacg gagcuaaggg ucggcuguuc ucuggccacc ucaagugccg acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucauugugua cugccgcauu uacguucaca     1500

aagguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gacgguccuu gcaaaauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggagauuaa uaacagcaaa uccugucauc acggaaagua ccgagaacuc caagaugaug     1680

cuggaacuag auccacccuu uggcgacagc uacauuguga ucggaguagg cgauaagaaa     1740

auaacucacc acuggcauag gucuggguca acacucggaa aggcguucag uaccacacug     1800

aaaggugcuc agcggcuugc cgcauugggc gacacugccu gggauuucgg gagcauugga     1860

ggcguguuua auuccaucgg ucgcgcuguu caccaagucu ucgggggcgc cuuuagaacc     1920

cuguucggag gcaugucuug gaucacgcag gggcucaugg gagcacugcu ucuguggaug     1980

ggcgugaacg cuagggaccg uucgauugcc uuggcguuuc ucgccaccgg uggggugcug     2040

gucuucuuag cuacaaacgu ucaugcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8283
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8283
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacacucgg aaaggcauuu     1800

aguaccacac ugaaaggugc ccagcgccuu gcugccuugg gcgauacugc augggacuuc     1860

gggagcauag gaggcguguu caauuccauu gguagagcug uacaccaagu guuugggggc     1920

gccuucagga cccuguuugg aggcaugucu uggaucacgc aggggcucau gggagcgcug     1980

cuucugugga ugggcguuaa cgcccgugau cggucgaucg cuuuggcauu ccucgccacc     2040

gguggggucc ugguguuuuu agcuacaaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8284
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  8284
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8285
<211>  2362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8285
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagaggcg gggcgcugac       60

accagcgugg ggauagucgg acugcuccuu acaacugcaa uggccgcgga aguuacgcgc      120

cgagguuccg cuuacuauau guaccuggau cguaaugacg ccggcgaggc aaucucuuuc      180

cccaccacau uggggaugaa caaaugcuau auucagauca uggauuuagg acacaugugu      240

gacgccacua ugucauacga augcccuaug cuggaugagg gcguggaacc agacgaugua      300

gacuguuggu gcaauaccac aaguaccugg guggucuaug guacuuguca ucacaagaaa      360

ggggaggcua gaaggagccg gcgcgcagug acacuaccgu cccauucuac cagaaagcuc      420

caaacgaggu cgcagacuug gcuggaguca cgggaauaca ccaagcaccu uauucgcguu      480

gagaacugga ucuuucgaaa ucccggauuc gcccuggcug ccgcggcaau agccugguug      540

cucggcagca guacauccca gaaagugauc uaucugguca ugauucugcu caucgcuccu      600

gccuacucua uuagaugcau cggcgugagc aacagggauu uuguagaagg gauguccgga      660

gguacuuggg uugacauagu guuagagcau ggcgggugug ucaccgugau ggcacaggau      720

aagccaacag uggacauuga gcuggucacc acgacuguuu caaauauggc ugaagugcgg      780

aguuacugcu augaggccag caucucugau auggcguccg acucacguug ucccacacaa      840

ggagaagccu accuugacaa acagagcgau acccaguaug uaugcaagcg cacuuuggug      900

gacagaggcu gggguaacgg guguggacug uucggcaaag gcagucuagu cacaugcgca      960

aaguuugcuu gcucuaagaa aaugaccggg aaguccauuc agccugagaa ucucgaauac     1020

aggaucaugc ugagcgugca cggaucgcaa cacucuggua ugaucguuaa cgauacgggc     1080

caugagaccg acgagaaucg ggccaaagug gaaauuacac caaacucacc ccgagcugag     1140

gccacucuug ggggauucgg cucccugggu uuggauugug aaccucgcac cgggcucgac     1200

uuuagugauc uguauuacuu aacaaugaau aacaagcacu ggcuggucca uaaagagugg     1260

uuccacgaca uaccacuccc guggcacgca ggcgccgaua cuggaacacc ccauuggaau     1320

aacaaggaag cucuugugga guuuaaagac gcgcacgcaa agagacagac cguaguugug     1380

cugggcagcc aggagggggc cguccauacc gcuuuggccg gagcacuaga agccgagaug     1440

gauggugcua agggcaggcu guccucuggg caccucaaau gccggcugaa gauggacaaa     1500

cuucgccuga agggagugag cuauucauug uguacugccg cauucacguu uacaaaaauc     1560

ccugcggaaa cccuccaugg cacugucacc guggagguuc aguacgcugg uacagacggg     1620

ccaugcaagg ugcccgccca aauggcagua gauaugcaga cccugacucc ugucggcaga     1680

uuaauuacag ccaacccagu gaucacggaa aguaccgaaa auuccaagau gaugcuggag     1740

cuagaucccc cguucggaga cagcuacaua guuauuggcg ugggggagaa aaagaucacu     1800

caccacuggc auaggucugg aucaacaauc gguaaggcuu uugaggccac cguccguggc     1860

gcaaaacgga uggcugugcu cggggacaca gccugggauu ucggaagugu aggcggugcg     1920

cugaacagcc uugggaaagg cauucaccag aucuucggag ccgcuuuuaa guccuuguuc     1980

ggcgggaugu cuugguuuuc gcaaauucug auaggaacuc uccugaugug gcuuggccug     2040

aauaccaaga acgguagcau cuccuugaug ugucucgcau uagggggagu gcugauuuuc     2100

cugucaacgg ccguuagugc ugacgucggc ugcucugugg auuuuagcaa augaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2340

ucuuuucaga gccaccagaa uu                                              2362


<210>  8286
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8286
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agaggcgggg cgcugacacc       60

agcgugggga uugucggacu gcuccuuaca acugcaaugg ccgcggaagu uacgcgccga      120

gguuccgcuu acuauaugua ccuggaucgu aaugacgccg gcgaggcaau aucuuucccc      180

accacauugg ggaugaacaa augcuauauc cagauuaugg auuuaggaca caugugugac      240

gccacuaugu cauacgaaug cccuaugcug gaugagggcg uggaaccaga cgauguagac      300

uguuggugca auaccacaag uaccugggug gucuauggua cuugucauca caagaaaggg      360

gaggcuagaa ggagccggcg cgcagugaca cuaccguccc auucuaccag aaagcuccaa      420

acgaggucgc agacuuggcu ggagucacgg gaauacacca agcaccuuau ccgcguugag      480

aacuggauuu uucgaaaucc cggauucgcc cuggcugccg cggcaaucgc cugguugcuc      540

ggcagcagua caucccagaa agugauauau cuggucauga uccugcucau ugcuccugcc      600

uacucuauca gaugcauugg cgugagcaac agggauuuug uagaagggau guccggaggu      660

acuuggguug acaucguguu agagcauggc ggguguguca ccgugauggc acaggauaag      720

ccaacagugg acauagagcu ggucaccacg acuguuucaa auauggcuga agugcggagu      780

uacugcuaug aggccagcau uucugauaug gcguccgacu cacguugucc cacacaagga      840

gaagccuacc uugacaaaca gagcgauacc caguauguau gcaagcgcac uuugguggac      900

agaggcuggg guaacgggug uggacuguuc ggcaaaggca gucuagucac augcgcaaag      960

uuugcuugcu cuaagaaaau gaccgggaag uccauccagc cugagaaucu cgaauacagg     1020

auuaugcuga gcgugcacgg aucgcaacac ucugguauga ucguuaacga uacgggccau     1080

gagaccgacg agaaucgggc caaaguggaa aucacaccaa acucaccccg agcugaggcc     1140

acucuugggg gauucggcuc ccuggguuug gauugugaac cucgcaccgg gcucgacuuu     1200

agugaucugu auuacuuaac aaugaauaac aagcacuggc ugguccauaa agagugguuc     1260

cacgacauuc cacucccgug gcacgcaggc gccgauacug gaacacccca uuggaauaac     1320

aaggaagcuc uuguggaguu uaaagacgcg cacgcaaaga gacagaccgu aguugugcug     1380

ggcagccagg agggggccgu ccauaccgcu uuggccggag cacuagaagc cgagauggau     1440

ggugcuaagg gcaggcuguc cucugggcac cucaaaugcc ggcugaagau ggacaaacuu     1500

cgccugaagg gagugagcua uucauugugu acugccgcau ucacguuuac aaaaauaccu     1560

gcggaaaccc uccauggcac ugucaccgug gagguucagu acgcugguac agacgggcca     1620

ugcaaggugc ccgcccaaau ggcaguagau augcagaccc ugacuccugu cggcagauua     1680

aucacagcca acccagugau uacggaaagu accgaaaauu ccaagaugau gcuggagcua     1740

gaucccccgu ucggagacag cuacaucguu auaggcgugg gggagaaaaa gauuacucac     1800

cacuggcaua ggucuggauc aacaaucggu aaggcuuuug aggccaccgu ccguggcgca     1860

aaacggaugg cugugcucgg ggacacagcc ugggauuucg gaaguguagg cggugcgcug     1920

aacagccuug ggaaaggcau ccaccagauu uucggagccg cuuuuaaguc cuuguucggc     1980

gggaugucuu gguuuucgca aauccugauu ggaacucucc ugauguggcu uggccugaau     2040

accaagaacg guagcauauc cuugaugugu cucgcauuag ggggagugcu gaucuuccug     2100

ucaacggccg uuagugcuga cgucggcugc ucuguggauu uuagcaaaug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  8287
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8287
gggagaaagc uuaccaugaa cgccagaaag gagaaaaaga ggcggggcgc ugacaccagc       60

guggggaucg ucggacugcu ccuuacaacu gcaauggccg cggaaguuac gcgccgaggu      120

uccgcuuacu auauguaccu ggaucguaau gacgccggcg aggcaauuuc uuuccccacc      180

acauugggga ugaacaaaug cuauauacag aucauggauu uaggacacau gugugacgcc      240

acuaugucau acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaua ccacaaguac cugggugguc uaugguacuu gucaucacaa gaaaggggag      360

gcuagaagga gccggcgcgc agugacacua ccgucccauu cuaccagaaa gcuccaaacg      420

aggucgcaga cuuggcugga gucacgggaa uacaccaagc accuuauucg cguugagaac      480

uggaucuuuc gaaaucccgg auucgcccug gcugccgcgg caauugccug guugcucggc      540

agcaguacau cccagaaagu gaucuaucug gucaugauac ugcucaucgc uccugccuac      600

ucuauuagau gcaucggcgu gagcaacagg gauuuuguag aagggauguc cggagguacu      660

uggguugaca uuguguuaga gcauggcggg ugugucaccg ugauggcaca ggauaagcca      720

acaguggaca ucgagcuggu caccacgacu guuucaaaua uggcugaagu gcggaguuac      780

ugcuaugagg ccagcauauc ugauauggcg uccgacucac guugucccac acaaggagaa      840

gccuaccuug acaaacagag cgauacccag uauguaugca agcgcacuuu gguggacaga      900

ggcuggggua acgggugugg acuguucggc aaaggcaguc uagucacaug cgcaaaguuu      960

gcuugcucua agaaaaugac cgggaagucc auucagccug agaaucucga auacaggauc     1020

augcugagcg ugcacggauc gcaacacucu gguaugauug uuaacgauac gggccaugag     1080

accgacgaga aucgggccaa aguggaaauc acaccaaacu caccccgagc ugaggccacu     1140

cuugggggau ucggcucccu ggguuuggau ugugaaccuc gcaccgggcu cgacuuuagu     1200

gaucuguauu acuuaacaau gaauaacaag cacuggcugg uccauaaaga gugguuccac     1260

gacaucccac ucccguggca cgcaggcgcc gauacuggaa caccccauug gaauaacaag     1320

gaagcucuug uggaguuuaa agacgcgcac gcaaagagac agaccguagu ugugcugggc     1380

agccaggagg gggccgucca uaccgcuuug gccggagcac uagaagccga gauggauggu     1440

gcuaagggca ggcuguccuc ugggcaccuc aaaugccggc ugaagaugga caaacuucgc     1500

cugaagggag ugagcuauuc auuguguacu gccgcauuca cguuuacaaa aauuccugcg     1560

gaaacccucc auggcacugu caccguggag guucaguacg cugguacaga cgggccaugc     1620

aaggugcccg cccaaauggc aguagauaug cagacccuga cuccugucgg cagauuaaua     1680

acagccaacc cagugaucac ggaaaguacc gaaaauucca agaugaugcu ggagcuagau     1740

cccccguucg gagacagcua cauuguuauc ggcguggggg agaaaaagau aacucaccac     1800

uggcauaggu cuggaucaac aauugguaag gcuuuugagg ccaccguccg uggcgcaaaa     1860

cggauggcug ugcucgggga cacagccugg gauuucggaa guguaggcgg ugcgcugaac     1920

agccuuggga aaggcaucca ccagaucuuc ggagccgcuu uuaaguccuu guucggcggg     1980

augucuuggu uuucgcaaau ucugaucgga acucuccuga uguggcuugg ccugaauacc     2040

aagaacggua gcauuuccuu gaugugucuc gcauuagggg gagugcugau auuccuguca     2100

acggccguua gugcugacgu cggcugcucu guggauuuua gcaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8288
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8288
gggagaaagc uuaccauggc cagaaaggag aaaaagaggc ggggcgcuga caccagcgug       60

gggaucgucg gacugcuccu uacaacugca auggccgcgg aaguuacgcg ccgagguucc      120

gcuuacuaua uguaccugga ucguaacgac gccggcgagg caauuucuuu ccccaccaca      180

uuggggauga auaaaugcua uauacagauc auggauuuag gacacaugug ugacgccacu      240

augucauacg aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg      300

ugcaacacca caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu      360

agaaggagcc ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccaaacgagg      420

ucgcagacuu ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaauugg      480

aucuuucgaa accccggauu cgcccuggcu gccgcggcaa uugccugguu gcucggcagc      540

aguacauccc agaaagugau cuaucugguc augauacugc ucaucgcucc ugccuacucu      600

auuagaugca ucggcgugag caauagggau uuuguagaag ggauguccgg agguacuugg      660

guugacauug uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca      720

guggacaucg agcuggucac cacgacuguu ucaaacaugg cugaagugcg gaguuacugc      780

uaugaggcca gcauaucuga uauggcgucc gacucacguu gucccacaca aggagaagcc      840

uaccuugaca aacagagcga uacccaguau guaugcaagc gcacuuuggu ggacagaggc      900

ugggguaaug gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu      960

ugcucuaaga aaaugaccgg gaaguccauu cagccugaga accucgaaua caggaucaug     1020

cugagcgugc acggaucgca acacucuggu augauuguua augauacggg ccaugagacc     1080

gacgagaacc gggccaaagu ggaaaucaca ccaaauucac cccgagcuga ggccacucuu     1140

gggggauucg gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau     1200

cuguauuacu uaacaaugaa caauaagcac uggcuggucc auaaagagug guuccacgac     1260

aucccacucc cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa     1320

gcucuugugg aguuuaaaga cgcgcacgca aagagacaga ccguaguugu gcugggcagc     1380

caggaggggg ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu     1440

aagggcaggc uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcauucacgu uuacaaaaau uccugcggaa     1560

acccuccaug gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag     1620

gugcccgccc aaauggcagu agauaugcag acccugacuc cugucggcag auuaauaaca     1680

gccaacccag ugaucacgga aaguaccgag aacuccaaga ugaugcugga gcuagauccc     1740

ccguucggag acagcuacau uguuaucggc gugggggaga aaaagauaac ucaccacugg     1800

cauaggucug gaucaacaau ugguaaggcu uuugaagcca ccguccgugg cgcaaaacgg     1860

auggcugugc ucggggacac agccugggau uucggaagug uaggcggugc gcugaauagc     1920

cuugggaaag gcauccacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug     1980

ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag     2040

aaugguagca uuuccuugau gugucucgca uuagggggag ugcugauauu ccugucaacg     2100

gccguuagug cugacgucgg cugcucugug gauuuuagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8289
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8289
gggagaaagc uuaccaugag aaaggagaaa aagaggcggg gcgccgacac cagcgugggg       60

aucgucggac ugcuccuuac aacugcuaug gcagccgaag uuacgcgccg agguuccgcg      120

uacuauaugu accuggaucg uaacgacgcu ggcgaggcca uuucuuuccc caccacauug      180

gggaugaaua aaugcuauau acagaucaug gauuuaggac acauguguga cgcaacuaug      240

ucauacgaau gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc      300

aacaccacaa guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggccaga      360

aggagccggc gcgcugugac acuaccgucc cauucuacca gaaagcucca aacgaggucg      420

cagacuuggc uggagucacg ggaauacacc aagcaccuua uucgcguuga gaauuggauc      480

uuucgaaacc ccggauucgc acuggccgcu gccgcgauug caugguugcu cggcagcagu      540

acaucccaga aagugaucua ucuggucaug auacugcuca ucgccccugc uuacucuauu      600

agaugcaucg gcgugagcaa uagggauuuu guagaaggga uguccggagg uacuuggguu      660

gacauugugu uagagcaugg cggguguguc accgugaugg cccaggauaa gccaacagug      720

gacaucgagc uggucaccac gacuguuuca aacauggcag aagugcggag uuacugcuau      780

gaggcuagca uaucugauau ggccuccgac ucacguuguc ccacacaagg agaagcguac      840

cuugacaaac agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg      900

gguaaugggu guggacuguu cggcaaaggc agucuaguca caugcgccaa guuugcaugc      960

ucuaagaaaa ugaccgggaa guccauucag ccugagaacc ucgaauacag gaucaugcug     1020

agcgugcacg gaucgcaaca cucugguaug auuguuaaug auacgggcca ugagaccgac     1080

gagaaccggg cuaaagugga aaucacacca aauucacccc gagccgaggc uacucuuggg     1140

ggauucggcu cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug     1200

uauuacuuaa caaugaacaa uaagcacugg cugguccaua aagagugguu ccacgacauc     1260

ccacucccgu ggcacgccgg cgcagauacu ggaacacccc auuggaacaa uaaggaagcc     1320

cuuguggagu uuaaagacgc ucacgcgaag agacagaccg uaguugugcu gggcagccag     1380

gagggggcag uccauaccgc cuuggcugga gcccuagaag cagagaugga uggugccaag     1440

ggcaggcugu ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag     1500

ggagugagcu auucauugug uacugcugcc uucacguuua caaaaauucc ugcagaaacc     1560

cuccauggca cugucaccgu ggagguucag uacgcgggua cagacgggcc augcaaggug     1620

cccgcucaaa uggccguaga uaugcagacc cugacuccug ucggcagauu aauaacagca     1680

aacccaguga ucacggaaag uaccgagaac uccaagauga ugcuggagcu agaucccccg     1740

uucggagaca gcuacauugu uaucggcgug ggggagaaaa agauaacuca ccacuggcau     1800

aggucuggau caacaauugg uaaagccuuu gaagcuaccg uccguggcgc caagcggaug     1860

gcagugcucg gggacacagc uugggauuuc ggaaguguag gcggugcccu gaauagccuu     1920

gggaaaggca uccaccagau cuucggagcg gccuuuaagu ccuuguucgg cgggaugucu     1980

ugguuuucgc aaauucugau cggaacucuc cugauguggc uuggccugaa caccaagaau     2040

gguagcauuu ccuugaugug ucucgcuuua gggggagugc ugauauuccu gucaacggca     2100

guuagugccg acgucggcug cucuguggau uuuagcaaau gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2220

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  8290
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8290
gggagaaagc uuaccaugaa ggagaaaaag agaaggggcg ccgacaccag cguggggauc       60

gucggacugc uccuuacaac ugcuauggca gccgaaguua cgcggcgcgg uuccgcguac      120

uauauguacc uggaucgaaa cgacgcuggc gaggccauuu cuuuccccac cacauugggg      180

augaauaaau gcuauauaca gaucauggau uuaggacaca ugugugacgc aacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcccguaga      360

agcaggcggg cugugacacu accgucccau ucuacccgca agcuccaaac gagaucgcag      420

acuuggcugg agucaaggga auacaccaag caccuuauuc ggguugagaa uuggaucuuu      480

cgcaaccccg gauucgcacu ggccgcugcc gcgauugcau gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugaua cugcucaucg ccccugcuua cucuauucga      600

ugcaucggcg ugagcaauag agauuuugua gaagggaugu ccggagguac uuggguugac      660

auuguguuag agcauggcgg gugugucacc gugauggccc aggauaagcc aacaguggac      720

aucgagcugg ucaccacgac uguuucaaac auggcagaag ugaggaguua cugcuaugag      780

gcuagcauau cugauauggc cuccgacuca cgguguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guauguaugc aagcguacuu ugguggaccg cggcuggggu      900

aaugggugug gacuguucgg caaaggcagu cuagucacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaccucg aauacagaau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauu guuaaugaua cgggccauga gaccgacgag     1080

aacagggcua aaguggaaau cacaccaaau ucaccccggg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagcgc cagaccguag uugugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

agacuguccu cugggcaccu caaaugcagg cugaagaugg acaaacuucg gcugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauuccugc agaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gccgcuuaau aacagcaaac     1680

ccagugauca cggaaaguac cgagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauuguuau cggcgugggg gagaaaaaga uaacucacca cuggcauaga     1800

ucuggaucaa caauugguaa agccuuugaa gcuaccguca ggggcgccaa gcguauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

agcauuuccu ugaugugucu cgcuuuaggg ggagugcuga uauuccuguc aacggcaguu     2100

agugccgacg ucggcugcuc uguggauuuu agcaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8291
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8291
gggagaaagc uuaccaugga gaagaaaaga aggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc uuacaacugc uauggcagcc gaaguuacgc ggcgcgguuc cgcguacuau      120

auguaccugg aucgaaacga cgcuggcgag gccauuucuu uccccaccac auuggggaug      180

aauaagugcu auauacagau cauggauuua ggacacaugu gugacgcaac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaaa agggggaggc ccguagaagc      360

aggcgggcug ugacacuacc gucccauucu acccgcaaac uccaaacgag aucgcagacu      420

uggcuggagu caagggaaua caccaagcac cuuauucggg uugagaauug gaucuuucgc      480

aaccccggau ucgcacuggc cgcugccgcg auugcauggu ugcucggcag caguacaucc      540

cagaagguga ucuaucuggu caugauacug cucaucgccc cugcuuacuc uauucgaugc      600

aucggcguga gcaauagaga uuuuguagaa gggauguccg gagguacuug gguugacauu      660

guguuagagc auggcgggug ugucaccgug auggcccagg auaaaccaac aguggacauc      720

gagcugguca ccacgacugu uucaaacaug gcagaaguga ggaguuacug cuaugaggcu      780

agcauaucug auauggccuc cgacucacgg ugucccacac aaggagaagc guaccuugac      840

aagcagagcg auacccagua uguaugcaaa cguacuuugg uggaccgcgg cugggguaau      900

ggguguggac uguucggcaa gggcagucua gucacaugcg ccaaauuugc augcucuaag      960

aagaugaccg ggaaauccau ucagccugag aaccucgaau acagaaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugauuguu aaugauacgg gccaugagac cgacgagaac     1080

agggcuaagg uggaaaucac accaaauuca ccccgggccg aggcuacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucga accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga acaauaaaca cuggcugguc cauaaggagu gguuccacga caucccacuc     1260

ccguggcacg ccggcgcaga uacuggaaca ccccauugga acaauaaaga agcccuugug     1320

gaguuuaagg acgcucacgc gaaacgccag accguaguug ugcugggcag ccaggagggg     1380

gcaguccaua ccgccuuggc uggagcccua gaagcagaga uggauggugc caagggcaga     1440

cuguccucug ggcaccucaa gugcaggcug aaaauggaca agcugcggcu gaaaggagug     1500

agcuauucau uguguacugc ugccuucacg uuuacaaaga uuccugcaga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcg gguacagacg ggccaugcaa agugcccgcu     1620

caaauggccg uagauaugca gacccugacu ccugucggcc gcuuaauaac agcaaaccca     1680

gugaucacgg aaaguaccga gaacuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uuguuaucgg cgugggggaa aagaaaauaa cucaccacug gcauagaucu     1800

ggaucaacaa uugguaaggc cuuugaggcu accgucaggg gcgccaaacg uauggcagug     1860

cucggggaca cagcuuggga uuucggaagu guaggcggug cccugaauag ccuugggaag     1920

ggcauccacc agaucuucgg agcggccuuu aaauccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugaucggaac ucuccugaug uggcuuggcc ugaacaccaa gaaugguagc     2040

auuuccuuga ugugucucgc ucuuggggga guguuaauau uccugucaac ggcaguuagu     2100

gccgacgucg gcugcucugu ggauuuuagc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  8292
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8292
gggagaaagc uuaccaugaa gaaaagaagg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuua caacugcuau ggcagccgag guuacgcggc gcgguuccgc guacuauaug      120

uaccuggauc gaaacgacgc uggcgaagcc auuucuuucc ccaccacauu ggggaugaau      180

aagugcuaua uacagaucau ggauuuagga cacaugugug acgcaacuau gucauacgag      240

ugcccuaugc uggaugaagg cguggagcca gacgauguag acuguuggug caacaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaaaaagg gggaagcccg uagaagcagg      360

cgggcuguga cacuaccguc ccauucuacc cgcaaacucc aaacgagauc gcagacuugg      420

cuggagucaa gggaguacac caagcaccuu auucggguug aaaauuggau cuuucgcaac      480

cccggauucg cacuggccgc ugccgcgauu gcaugguugc ucggcagcag uacaucccag      540

aaggugaucu aucuggucau gauacugcuc aucgccccug cuuacucuau ucgaugcauc      600

ggcgugagca auagagauuu uguagagggg auguccggag guacuugggu ugacauugug      660

uuagaacaug gcgggugugu caccgugaug gcccaggaua aaccaacagu ggacaucgag      720

cuggucacca cgacuguuuc aaacauggca gaggugagga guuacugcua ugaagcuagc      780

auaucugaua uggccuccga cucacggugu cccacacaag gagaggcgua ccuugacaag      840

cagagcgaua cccaguaugu augcaaacgu acuuuggugg accgcggcug ggguaauggg      900

uguggacugu ucggcaaggg cagucuaguc acaugcgcca aauuugcaug cucuaagaag      960

augaccggga aauccauuca gccugaaaac cucgaguaca gaaucaugcu gagcgugcac     1020

ggaucgcaac acucugguau gauuguuaau gauacgggcc augaaaccga cgagaacagg     1080

gcuaaggugg agaucacacc aaauucaccc cgggccgaag cuacucuugg gggauucggc     1140

ucccuggguu uggauuguga gccucgaacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgacau cccacucccg     1260

uggcacgccg gcgcagauac uggaacaccc cauuggaaca auaaagaggc ccuuguggaa     1320

uuuaaggacg cucacgcgaa acgccagacc guaguugugc ugggcagcca ggagggggca     1380

guccauaccg ccuuggcugg agcccuagag gcagaaaugg auggugccaa gggcagacug     1440

uccucugggc accucaagug caggcugaaa auggacaagc ugcggcugaa aggagugagc     1500

uauucauugu guacugcugc cuucacguuu acaaagauuc cugcagagac ccuccauggc     1560

acugucaccg uggaaguuca guacgcgggu acagacgggc caugcaaagu gcccgcucaa     1620

auggccguag auaugcagac ccugacuccu gucggccgcu uaauaacagc aaacccagug     1680

aucacggaga guaccgaaaa cuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacauug uuaucggcgu gggggagaag aaaauaacuc accacuggca uagaucugga     1800

ucaacaauug guaaggccuu ugaagcuacc gucaggggcg ccaaacguau ggcagugcuc     1860

ggggacacag cuugggauuu cggaagugua ggcggugccc ugaauagccu ugggaagggc     1920

auccaccaga ucuucggagc ggccuuuaaa uccuuguucg gcgggauguc uugguuuucg     1980

caaauucuga ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu     2040

uccuugaugu gucucgcucu ugggggagug uuaauauucc ugucaacggc aguuagugcc     2100

gacgucggcu gcucugugga uuuuagcaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  8293
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8293
gggagaaagc uuaccaugaa gagaaggggc gccgacacca gcguggggau cgucggacug       60

cuccuuacaa cugcuauggc agccgagguu acgcggcgcg guuccgcgua cuauauguac      120

cuggaucgaa acgacgcugg cgaagccauu ucuuucccca ccacauuggg gaugaauaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg caacuauguc auacgagugc      240

ccuaugcugg augaaggcgu ggagccagac gauguagacu guuggugcaa caccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aagcccguag aagcaggcgg      360

gcugugacac uaccguccca uucuacccgc aagcuccaaa cgagaucgca gacuuggcug      420

gagucaaggg aguacaccaa acaccuuauu cggguugaaa auuggaucuu ucgcaacccc      480

ggauucgcac uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaag      540

gugaucuauc uggucaugau acugcucauc gccccugcuu acucuauucg augcaucggc      600

gugagcaaua gagauuuugu agaggggaug uccggaggua cuuggguuga cauuguguua      660

gaacauggcg ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa cauggcagag gugaggaguu acugcuauga agcuagcaua      780

ucugauaugg ccuccgacuc acgguguccc acacaaggag aggcguaccu ugacaaacag      840

agcgauaccc aguauguaug caagcguacu uugguggacc gcggcugggg uaaugggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaaaaagaug      960

accgggaagu ccauucagcc ugaaaaccuc gaguacagaa ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaaugau acgggccaug aaaccgacga gaacagggcu     1080

aaaguggaga ucacaccaaa uucaccccgg gccgaagcua cucuuggggg auucggcucc     1140

cuggguuugg auugugagcc ucgaaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaacaaua agcacuggcu gguccauaaa gaaugguucc acgacauccc acucccgugg     1260

cacgccggcg cagauacugg aacaccccau uggaacaaua aggaggcccu uguggaauuu     1320

aaagacgcuc acgcgaagcg ccagaccgua guugugcugg gcagccagga gggggcaguc     1380

cauaccgccu uggcuggagc ccuagaggca gaaauggaug gugccaaagg cagacugucc     1440

ucugggcacc ucaagugcag gcugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucauugugua cugcugccuu cacguuuaca aaaauuccug cagagacccu ccauggcacu     1560

gucaccgugg aaguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug     1620

gccguagaua ugcagacccu gacuccuguc ggccgcuuaa uaacagcaaa cccagugauc     1680

acggagagua ccgaaaacuc caaaaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaagaag auaacucacc acuggcauag aucuggauca     1800

acaauuggua aagccuuuga agcuaccguc aggggcgcca agcguauggc agugcucggg     1860

gacacagcuu gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc     1920

caccagaucu ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaca ccaaaaaugg uagcauuucc     2040

uugauguguc ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac     2100

gucggcugcu cuguggauuu uagcaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8294
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8294
gggagaaagc uuaccaugag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug cuauggcagc cgagguuacg cggcgcgguu ccgcguacua uauguaccug      120

gaucgaaacg acgcuggcga agccauuucu uuccccacca cauuggggau gaauaagugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgagugcccu      240

augcuggaug aaggcgugga gccagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaaa aagggggaag cccguagaag caggcgggcu      360

gugacacuac cgucccauuc uacccgcaaa cuccaaacga gaucgcagac uuggcuggag      420

ucaagggagu acaccaagca ccuuauucgg guugaaaauu ggaucuuucg caaccccgga      480

uucgcacugg ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug      600

agcaauagag auuuuguaga ggggaugucc ggagguacuu ggguugacau uguguuagaa      660

cauggcgggu gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcagaggug aggaguuacu gcuaugaagc uagcauaucu      780

gauauggccu ccgacucacg gugucccaca caaggagagg cguaccuuga caagcagagc      840

gauacccagu auguaugcaa acguacuuug guggaccgcg gcugggguaa ugggugugga      900

cuguucggca agggcagucu agucacaugc gccaaauuug caugcucuaa gaaaaugacc      960

gggaagucca uucagccuga aaaccucgag uacagaauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaaa ccgacgagaa cagggcuaag     1080

guggagauca caccaaauuc accccgggcc gaagcuacuc uugggggauu cggcucccug     1140

gguuuggauu gugagccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu ccauaaggaa ugguuccacg acaucccacu cccguggcac     1260

gccggcgcag auacuggaac accccauugg aacaauaaag aggcccuugu ggaauuuaag     1320

gacgcucacg cgaaacgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau     1380

accgccuugg cuggagcccu agaggcagaa auggauggug ccaagggcag acuguccucu     1440

gggcaccuca aaugcaggcu gaagauggac aagcugcggc ugaaaggagu gagcuauuca     1500

uuguguacug cugccuucac guuuacaaag auuccugcag agacccucca uggcacuguc     1560

accguggaag uucaguacgc ggguacagac gggccaugca aagugcccgc ucaaauggcc     1620

guagauaugc agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca     1800

auugguaagg ccuuugaagc uaccgucagg ggcgccaaac guauggcagu gcucggggac     1860

acagcuuggg auuucggaag uguaggcggu gcccugaaua gccuugggaa gggcauccac     1920

cagaucuucg gagcggccuu uaaauccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cucuuggggg aguguuaaua uuccugucaa cggcaguuag ugccgacguc     2100

ggcugcucug uggauuuuag caaaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8295
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8295
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga gguuacgagg cgggguuccg cguacuauau guaccuggau      120

cgcaacgacg cuggcgaagc cauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga gugcccuaug      240

cuggaugaag gcguggagcc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaagccc gacguagcag aagggcugug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaguca      420

agagaguaca ccaagcaccu uauuaggguu gaaaauugga ucuuucggaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaggg gauguccgga gguacuuggg uugacauugu guuagaacau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaggugaga aguuacugcu augaagcuag cauaucugau      780

auggccuccg acucaaggug ucccacacaa ggagaggcgu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugaaaa ccucgaguac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugaaaccg acgagaacag agcuaaggug     1080

gagaucacac caaauucccc cagggccgaa gcuacucuug ggggauucgg cucacugggu     1140

uuggauugug agccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggaaugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaagagg cccuugugga auuuaaggac     1320

gcucacgcga aacgacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga ggcagaaaug gauggugcca agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaagauu ccugcagaga cccuccaugg cacugucacc     1560

guggaaguuc aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag caaacccagu gaucacggag     1680

aguaccgaaa acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggccu uugaagcuac cgucagaggc gccaaaagga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuc uugggggagu guuaauauuc cugucaacgg caguuagugc cgacgucggc     2100

ugcucugugg auuuuagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8296
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8296
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaggu uacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

aacgacgcug gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug      240

gaugaaggcg uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caaaaagggg gaagcccgcc gaagccguag agcugugaca      360

cuaccguccc auucuaccag gaaacuccaa acgcggucgc agacuuggcu ggagucacgc      420

gaguacacca agcaccuuau uagaguugaa aauuggaucu uuaggaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc ggugcaucgg cgugagcaau      600

cgcgauuuug uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga ggugcgaagu uacugcuaug aagcuagcau aucugauaug      780

gccuccgacu caagaugucc cacacaagga gaggcguacc uugacaagca gagcgauacc      840

caguauguau gcaaaaggac uuugguggac cggggcuggg guaaugggug uggacuguuc      900

ggcaagggca gucuagucac augcgccaaa uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugaaaaccu cgaguaccgu aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gaaaccgacg agaaccgcgc uaagguggag     1080

aucacaccaa auucccccag agccgaagcu acucuugggg gauucggcuc acuggguuug     1140

gauugugagc cuaggaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aaacacuggc ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu     1320

cacgcgaaac ggcagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaggc agaaauggau ggugccaagg gccgacuguc cucugggcac     1440

cucaaaugcc gccugaagau ggacaagcug agacugaaag gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug     1560

gaaguucagu acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggcagguua auaacagcaa acccagugau cacggagagu     1680

accgaaaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcauc ggucuggauc aacaauuggu     1800

aaggccuuug aagcuaccgu ccgcggcgcc aaaagaaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc     1920

uucggagcgg ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcucuug ggggaguguu aauauuccug ucaacggcag uuagugccga cgucggcugc     2100

ucuguggauu uuagcaaaug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8297
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8297
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccuuacaacu       60

gcuauggcag ccgagguuac gagaagggga uccgcguacu auauguaccu ggaucggaac      120

gacgcuggug aagccauuuc uuuccccacc acauugggca ugaauaagug cuauauacag      180

aucauggauu uagggcacau gugugacgca acuaugucau acgagugccc uaugcuggau      240

gaaggagugg agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uauggcacuu gucaucacaa aaagggugaa gcccgccgaa gccguagagc ugugacacua      360

ccgucccauu cuaccaggaa acuccaaacg cggucgcaga cuuggcugga gucacgcgag      420

uacaccaagc accuuauuag aguugaaaau uggaucuuua ggaaccccgg guucgcacug      480

gccgcugccg cgauugcaug guugcucgga agcaguacau cccagaaagu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucggu gcaucggcgu gagcaaucgc      600

gauuuuguag agggcauguc cgggggaacu uggguugaca uuguguuaga acaugguggc      660

ugugucaccg ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaggu gcgaaguuac ugcuaugaag cuagcauauc ugauauggcc      780

uccgacucaa gaugucccac acaaggggag gcguaccuug acaagcagag cgauacccag      840

uauguaugca aaaggacuuu gguggaccgg ggauggggca augguugugg gcuguucgga      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaaaaugac cggcaagucc      960

auucagccug aaaaccucga guaccguauc augcugagcg ugcacggguc gcaacacucu     1020

ggaaugauug uuaaugauac gggucaugaa accgacgaga accgcgcuaa gguggagauc     1080

acaccaaauu cccccagagc cgaagcuacu cuuggcgggu ucggaucacu gggcuuggau     1140

ugugagccua ggaccggucu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gacaucccac ucccguggca cgccggggca     1260

gauacuggca caccccauug gaacaauaaa gaggcccuug uggaauuuaa ggacgcucac     1320

gcgaaacggc agaccguagu ugugcuggga agccaggagg gcgcagucca uaccgccuug     1380

gcuggggccc uagaggcaga aauggaugga gccaaggguc gacuguccuc uggccaccuc     1440

aaaugccgcc ugaagaugga caaacuuaga cugaaggggg ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gagacccucc auggaacugu caccguggaa     1560

guucaguacg cgggcacaga cgguccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg gagguuaaua acagcaaacc cagugaucac ggagaguacc     1680

gaaaacucca agaugaugcu ggagcuagau cccccguucg gcgacagcua cauuguuauc     1740

ggagugggcg agaaaaagau aacucaccac uggcaucggu cugggucaac aauuggaaag     1800

gccuuugaag cuaccguccg cggugccaaa agaauggcag ugcucggcga cacagcuugg     1860

gauuucggga guguaggagg cgcccugaau agccuuggua aggggaucca ccagaucuuc     1920

ggcgcggccu uuaaauccuu guucggaggc augucuuggu uuucgcaaau ucugaucggg     1980

acucuccuga uguggcuugg acugaacacc aagaauggca gcauuuccuu gaugugucuc     2040

gcucugggug ggguguuaau auuccuguca acggcaguua gugccgacgu cggaugcucu     2100

guggauuuua gcaaaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8298
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8298
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu uacaacugcc       60

auggcugcag agguuacgag aaggggaucc gccuacuaua uguaccugga ucggaacgac      120

gcgggugaag cuauuucuuu ccccaccaca uugggcauga auaagugcua uauacagauc      180

auggauuuag ggcacaugug ugacgccacu augucauacg agugcccuau gcuggaugaa      240

ggaguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

ggcacuuguc aucacaaaaa gggugaagca cgccgaagcc guagagccgu gacacuaccg      360

ucccauucua ccaggaaacu ccaaacgcgg ucgcagacuu ggcuggaguc acgcgaguac      420

accaagcacc uuauuagagu ugaaaauugg aucuuuagga accccggguu cgcucuggca      480

gccgcugcca uugcgugguu gcucggaagc aguacauccc agaaagugau cuaucugguc      540

augauacugc ucaucgcacc cgccuacucu auucggugca ucggcgugag caaucgcgau      600

uuuguagagg gcauguccgg gggaacuugg guugacauug uguuagaaca ugguggcugu      660

gucaccguga uggcucagga uaagccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg ccgaggugcg aaguuacugc uaugaagcaa gcauaucuga uauggcuucc      780

gacucaagau gucccacaca aggggaggcc uaccuugaca agcagagcga uacccaguau      840

guaugcaaaa ggacuuuggu ggaccgggga uggggcaaug guugugggcu guucggaaag      900

ggcagucuag ucacaugcgc gaaauuugcc ugcucuaaga aaaugaccgg caaguccauu      960

cagccugaaa accucgagua ccguaucaug cugagcgugc acgggucgca acacucugga     1020

augauuguua augauacggg ucaugaaacc gacgagaacc gcgcaaaggu ggagaucaca     1080

ccaaauuccc ccagagcuga agccacucuu ggcggguucg gaucacuggg cuuggauugu     1140

gagccuagga ccggucucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc uggggccgau     1260

acuggcaccc cucauuggaa caauaaagag gcacuugugg aauuuaagga cgcccacgcu     1320

aaacggcaga caguaguugu gcugggaagc caggagggcg cgguccauac cgcauuggcc     1380

ggggcucuag aggccgaaau ggauggagca aagggucgac uguccucugg ccaccucaaa     1440

ugccgccuga agauggacaa acuuagacug aaggggguga gcuauucauu guguacugcc     1500

gcuuucacgu uuacaaagau uccugccgag acccuccaug gaacugucac cguggaaguu     1560

caguacgcag gcacagacgg uccaugcaaa gugcccgcgc aaauggcugu agauaugcag     1620

acccugacuc cugucgggag guuaauaaca gccaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggcg acagcuacau uguuaucgga     1740

gugggcgaga aaaagauaac ucaccacugg caucggucug ggucaacaau uggaaaggca     1800

uuugaagcca ccguccgcgg ugcuaaaaga auggccgugc ucggcgacac agcaugggau     1860

uucgggagug uaggaggcgc ucugaauagc cuugguaagg ggauccacca gaucuucggc     1920

gccgcguuua aauccuuguu cggaggcaug ucuugguuuu cgcaaauucu gaucgggacu     1980

cuccugaugu ggcuuggacu gaacaccaag aauggcagca uuuccuugau gugucucgcc     2040

cugggugggg uguuaauauu ccugucaacg gcuguuagug cagacgucgg augcucugug     2100

gauuuuagca aaugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8299
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8299
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcag augucggaug cucuguggac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8300
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8300
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagccg uuacgagaag gggauccgcg uacuauaugu accuggaccg gaacgaugcu      120

ggugaggcca uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcaacuaug ucauacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaggcccgc cgaagccgua gagcugugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggaaucacg cgaguacacc      420

aagcaccuua uuagaguuga gaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagaaggca uguccggggg aacuuggguu gauauugugu uagagcaugg uggcuguguc      660

accgugaugg cccaggacaa gccaacagug gauaucgaac uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaggcuagca uaucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaagcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugagaacc ucgaauaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugagaccgau gaaaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaggc uacucuuggc ggguucggau cacugggcuu ggacugugaa     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggagugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacacu     1260

ggcacacccc auuggaacaa uaaagaagcc cuuguggagu uuaaggaugc ucacgcgaaa     1320

cggcagaccg uaguugugcu gggaagccag gaaggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagagaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagaaacc cuccauggaa cugucaccgu ggagguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaggcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccg augucggaug cucuguggac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8301
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8301
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccgc gguuacgagg cgggguuccg cuuacuauau guaccuggau      120

cgcaacgacg ccggcgaggc aauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgccacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaggcuc gacguagcag aagggcagug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaauca      420

agagaguaca ccaagcaccu uauuaggguu gagaauugga ucuuucggaa ccccggauuc      480

gcccuggcug ccgcggcaau ugccugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgcuccu gccuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcacaggau aagccaacag uggacaucga acuggucacc      720

acgacuguuu caaacauggc ugaggugaga aguuacugcu augaggccag cauaucugau      780

auggcguccg acucaaggug ucccacacaa ggagaagccu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgca aaauuugcuu gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgaaaacag agccaaggug     1080

gagaucacac caaauucccc cagggcugag gccacucuug ggggauucgg cucacugggu     1140

uuggauugug aaccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgca     1260

ggcgccgaua cuggaacacc ccauuggaac aauaaagaag cucuugugga guuuaaggac     1320

gcgcacgcaa aacgacagac cguaguugug cugggcagcc aggaaggggc cguccauacc     1380

gcuuuggccg gagcacuaga ggccgagaug gauggugcua agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugccg cauucacguu uacaaagauu ccugcggaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcugg uacagacggg ccaugcaaag ugcccgccca aauggcagua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag ccaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggaa cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggcuu uugaggccac cgucagaggc gcaaaaagga uggcugugcu cggggacaca     1860

gccugggauu ucggaagugu aggcggugcg cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag ccgcuuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcac uugggggagu guuaauauuc cugucaacgg ccguuagugc ugacgucggc     2100

ugcucugugg auuuuagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8302
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8302
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagguguuac ucgacguggc      120

ucagccuacu auauguaccu ggauagaaac gacgcugggg aggcaauuag uuuucccacg      180

acccuaggaa ugaauaaaug cuauauacaa aucauggauc ucggccauau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggugugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugggaccu gucaccauaa gaaaggagag      360

gcgaggcggu cucgcagagc ugugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg cuuugcccug gcagccgcug caauugccug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc uccugccuac      600

uccauuaggu gcaucggggu gucaaaucgg gauuucguag aaggaaugag ugguggcaca      660

uggguugaca uuguguugga gcacggggga ugugucacug ugauggcgca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggcagaggu gcguucuuac      780

ugcuaugagg ccuccauauc agauauggcu agcgacaguc gcugucccac ucagggcgaa      840

gccuaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

gguuggggga auggaugugg ccuguuuggc aaaggguccc uugucacuug cgcaaaguuc      960

gcuugcagca aaaagaugac aggaaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacgguuc ccagcauuca ggcaugauug uuaaugauac cgggcacgag     1080

acggacgaaa accgagccaa aguggagauc accccaaaua guccccgcgc ggaggccaca     1140

cucggaggcu uugguagccu gggguuagau ugugaaccua gaacuggccu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcaggagcu gauacaggca cuccccacug gaacaauaag     1320

gaagcccuag uggaguucaa agacgcucau gccaagaggc agaccguagu ugugcugggg     1380

ucucaggaag gagcagucca cacagcccuc gcuggugcgc uggaggcaga gauggauggc     1440

gccaaagggc ggcuuagcuc aggacaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggcg ugaguuauuc cuuauguacc gcugccuuua cuuucacgaa aauuccugca     1560

gaaacacugc acgguaccgu cacuguggag guucaauacg ccgggaccga cggcccaugc     1620

aaggugcccg cucagauggc cguagauaug cagacacuaa ccccugucgg aaggcucaua     1680

acugcaaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gcgacucaua cauuguuauc ggggugggag agaagaagau aacccaccau     1800

uggcaccgua gugguagcac uauuggcaaa gcguucgagg cuacaguccg aggggccaag     1860

cggauggcag uguugggaga caccgccugg gauuucggcu ccguaggugg ggcucucaau     1920

ucucugggca aaggaaucca ucaaaucuuu ggcgccgcau ucaagucgcu guuuggggga     1980

augagcuggu ucucccagau ucuuaucggc acacuguuga uguggcucgg ucugaacacu     2040

aaaaaugggu caauuaguuu aaugugucug gcucuaggag gcgugcucau auuucugucu     2100

accgccguua gcgcggacgu cgguugcucc guggauuucu caaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8303
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8303
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagccguuac ucgacguggu      120

ucagcuuacu auauguaccu ggauagaaac gacgcaggcg aggccauuag uuuucccacg      180

acccuaggga ugaauaaaug cuauauacaa aucauggauc ucggacauau gugugacgcg      240

acaaugagcu acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugguaccu gucaccauaa gaaaggggag      360

gcuaggcggu cucgcagagc cgugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg auuugcacug gccgcugcag ccauugcuug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc cccugcguac      600

uccauuaggu gcaucggcgu gucaaaucgg gauuucguag aagggaugag uggagguaca      660

uggguugaca uuguguugga gcacggcggg ugugucacug ugauggcaca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggccgaggu gcguucuuac      780

ugcuaugagg cuuccauauc agauauggcc agcgacaguc gcugucccac ucagggagaa      840

gcauaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

ggcuggggua augggugugg acuguuuggc aaaggcuccc uugucacuug cgcuaaguuc      960

gccugcagca aaaagaugac agggaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacggauc ccagcauuca gguaugauug uuaaugauac cggccacgag     1080

acggacgaaa accgagcgaa aguggagauc accccaaaua guccccgcgc cgaggcaaca     1140

cucgggggau uuggcagccu ggguuuagau ugugaaccua gaacugggcu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcuggcgcc gauacaggaa cuccccacug gaacaauaag     1320

gaagcucuag uggaguucaa agacgcccau gcaaagaggc agaccguagu ugugcugggc     1380

ucucaggaag gggccgucca cacagcucuc gcgggagcac uggaggccga gauggauggu     1440

gcuaaaggcc ggcuuagcuc agggcaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggag ugaguuauuc cuuauguacc gccgcauuua cuuucacgaa aauuccugcc     1560

gaaacacugc acggcaccgu cacuguggag guucaauacg cugguaccga cgggccaugc     1620

aaggugcccg cccagauggc aguagauaug cagacacuaa ccccugucgg caggcucaua     1680

acugcgaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gagacucaua cauuguuauc ggcguggggg agaagaagau aacccaccau     1800

uggcaccgua guggaagcac uauugguaag gcuuucgagg ccacaguccg gggcgcaaaa     1860

cgaauggccg uguuggggga caccgcuugg gauuucggau ccguaggcgg ugcccucaau     1920

ucucugggga aaggcaucca ucaaaucuuu ggagcagcuu ucaagucgcu guuuggcggg     1980

augagcuggu ucucccagau ucuuaucgga acacuguuga uguggcucgg ccugaacacu     2040

aaaaaugguu caauuaguuu aaugugucug gcccuagggg gagugcucau auuucugucu     2100

accgcgguua gcgccgacgu cggcugcucc guggauuucu caaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8304
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8304
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8305
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8305
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagaggcg gggcgcugac       60

accagcgugg ggauagucgg acugcuccuu acaacugcaa uggccgcgga aguuacgcgc      120

cgagguuccg cuuacuauau guaccuggau cguaaugacg ccggcgaggc aaucucuuuc      180

cccaccacau uggggaugaa caaaugcuau auucagauca uggauuuagg acacaugugu      240

gacgccacua ugucauacga augcccuaug cuggaugagg gcguggaacc agacgaugua      300

gacuguuggu gcaauaccac aaguaccugg guggucuaug guacuuguca ucacaagaaa      360

ggggaggcua gaaggagccg gcgcgcagug acacuaccgu cccauucuac cagaaagcuc      420

caaacgaggu cgcagacuug gcuggaguca cgggaauaca ccaagcaccu uauucgcguu      480

gagaacugga ucuuucgaaa ucccggauuc gcccuggcug ccgcggcaau agccugguug      540

cucggcagca guacauccca gaaagugauc uaucugguca ugauucugcu caucgcuccu      600

gccuacucua uuagaugcau cggcgugagc aacagggauu uuguagaagg gauguccgga      660

gguacuuggg uugacauagu guuagagcau ggcgggugug ucaccgugau ggcacaggau      720

aagccaacag uggacauuga gcuggucacc acgacuguuu caaauauggc ugaagugcgg      780

aguuacugcu augaggccag caucucugau auggcguccg acucacguug ucccacacaa      840

ggagaagccu accuugacaa acagagcgau acccaguaug uaugcaagcg cacuuuggug      900

gacagaggcu gggguaacgg guguggacug uucggcaaag gcagucuagu cacaugcgca      960

aaguuugcuu gcucuaagaa aaugaccggg aaguccauuc agccugagaa ucucgaauac     1020

aggaucaugc ugagcgugca cggaucgcaa cacucuggua ugaucguuaa cgauacgggc     1080

caugagaccg acgagaaucg ggccaaagug gaaauuacac caaacucacc ccgagcugag     1140

gccacucuug ggggauucgg cucccugggu uuggauugug aaccucgcac cgggcucgac     1200

uuuagugauc uguauuacuu aacaaugaau aacaagcacu ggcuggucca uaaagagugg     1260

uuccacgaca uaccacuccc guggcacgca ggcgccgaua cuggaacacc ccauuggaau     1320

aacaaggaag cucuugugga guuuaaagac gcgcacgcaa agagacagac cguaguugug     1380

cugggcagcc aggagggggc cguccauacc gcuuuggccg gagcacuaga agccgagaug     1440

gauggugcua agggcaggcu guccucuggg caccucaaau gccggcugaa gauggacaaa     1500

cuucgccuga agggagugag cuauucauug uguacugccg cauucacguu uacaaaaauc     1560

ccugcggaaa cccuccaugg cacugucacc guggagguuc aguacgcugg uacagacggg     1620

ccaugcaagg ugcccgccca aauggcagua gauaugcaga cccugacucc ugucggcaga     1680

uuaauuacag ccaacccagu gaucacggaa aguaccgaaa auuccaagau gaugcuggag     1740

cuagaucccc cguucggaga cagcuacaua guuauuggcg ugggggagaa aaagaucacu     1800

caccacuggc auaggucugg aucaacaauc gguaaggcuu uugaggccac cguccguggc     1860

gcaaaacgga uggcugugcu cggggacaca gccugggauu ucggaagugu aggcggugcg     1920

cugaacagcc uugggaaagg cauucaccag aucuucggag ccgcuuuuaa guccuuguuc     1980

ggcgggaugu cuugguuuuc gcaaauucug auaggaacuc uccugaugug gcuuggccug     2040

aauaccaaga acgguagcau cuccuugaug ugucucgcau uagggggagu gcugauuuuc     2100

cugucaacgg ccguuagugc ugacgucggc ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  8306
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8306
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agaggcgggg cgcugacacc       60

agcgugggga uugucggacu gcuccuuaca acugcaaugg ccgcggaagu uacgcgccga      120

gguuccgcuu acuauaugua ccuggaucgu aaugacgccg gcgaggcaau aucuuucccc      180

accacauugg ggaugaacaa augcuauauc cagauuaugg auuuaggaca caugugugac      240

gccacuaugu cauacgaaug cccuaugcug gaugagggcg uggaaccaga cgauguagac      300

uguuggugca auaccacaag uaccugggug gucuauggua cuugucauca caagaaaggg      360

gaggcuagaa ggagccggcg cgcagugaca cuaccguccc auucuaccag aaagcuccaa      420

acgaggucgc agacuuggcu ggagucacgg gaauacacca agcaccuuau ccgcguugag      480

aacuggauuu uucgaaaucc cggauucgcc cuggcugccg cggcaaucgc cugguugcuc      540

ggcagcagua caucccagaa agugauauau cuggucauga uccugcucau ugcuccugcc      600

uacucuauca gaugcauugg cgugagcaac agggauuuug uagaagggau guccggaggu      660

acuuggguug acaucguguu agagcauggc ggguguguca ccgugauggc acaggauaag      720

ccaacagugg acauagagcu ggucaccacg acuguuucaa auauggcuga agugcggagu      780

uacugcuaug aggccagcau uucugauaug gcguccgacu cacguugucc cacacaagga      840

gaagccuacc uugacaaaca gagcgauacc caguauguau gcaagcgcac uuugguggac      900

agaggcuggg guaacgggug uggacuguuc ggcaaaggca gucuagucac augcgcaaag      960

uuugcuugcu cuaagaaaau gaccgggaag uccauccagc cugagaaucu cgaauacagg     1020

auuaugcuga gcgugcacgg aucgcaacac ucugguauga ucguuaacga uacgggccau     1080

gagaccgacg agaaucgggc caaaguggaa aucacaccaa acucaccccg agcugaggcc     1140

acucuugggg gauucggcuc ccuggguuug gauugugaac cucgcaccgg gcucgacuuu     1200

agugaucugu auuacuuaac aaugaauaac aagcacuggc ugguccauaa agagugguuc     1260

cacgacauuc cacucccgug gcacgcaggc gccgauacug gaacacccca uuggaauaac     1320

aaggaagcuc uuguggaguu uaaagacgcg cacgcaaaga gacagaccgu aguugugcug     1380

ggcagccagg agggggccgu ccauaccgcu uuggccggag cacuagaagc cgagauggau     1440

ggugcuaagg gcaggcuguc cucugggcac cucaaaugcc ggcugaagau ggacaaacuu     1500

cgccugaagg gagugagcua uucauugugu acugccgcau ucacguuuac aaaaauaccu     1560

gcggaaaccc uccauggcac ugucaccgug gagguucagu acgcugguac agacgggcca     1620

ugcaaggugc ccgcccaaau ggcaguagau augcagaccc ugacuccugu cggcagauua     1680

aucacagcca acccagugau uacggaaagu accgaaaauu ccaagaugau gcuggagcua     1740

gaucccccgu ucggagacag cuacaucguu auaggcgugg gggagaaaaa gauuacucac     1800

cacuggcaua ggucuggauc aacaaucggu aaggcuuuug aggccaccgu ccguggcgca     1860

aaacggaugg cugugcucgg ggacacagcc ugggauuucg gaaguguagg cggugcgcug     1920

aacagccuug ggaaaggcau ccaccagauu uucggagccg cuuuuaaguc cuuguucggc     1980

gggaugucuu gguuuucgca aauccugauu ggaacucucc ugauguggcu uggccugaau     2040

accaagaacg guagcauauc cuugaugugu cucgcauuag ggggagugcu gaucuuccug     2100

ucaacggccg uuagugcuga cgucggcugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  8307
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8307
gggagaaagc uuaccaugaa cgccagaaag gagaaaaaga ggcggggcgc ugacaccagc       60

guggggaucg ucggacugcu ccuuacaacu gcaauggccg cggaaguuac gcgccgaggu      120

uccgcuuacu auauguaccu ggaucguaau gacgccggcg aggcaauuuc uuuccccacc      180

acauugggga ugaacaaaug cuauauacag aucauggauu uaggacacau gugugacgcc      240

acuaugucau acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaua ccacaaguac cugggugguc uaugguacuu gucaucacaa gaaaggggag      360

gcuagaagga gccggcgcgc agugacacua ccgucccauu cuaccagaaa gcuccaaacg      420

aggucgcaga cuuggcugga gucacgggaa uacaccaagc accuuauucg cguugagaac      480

uggaucuuuc gaaaucccgg auucgcccug gcugccgcgg caauugccug guugcucggc      540

agcaguacau cccagaaagu gaucuaucug gucaugauac ugcucaucgc uccugccuac      600

ucuauuagau gcaucggcgu gagcaacagg gauuuuguag aagggauguc cggagguacu      660

uggguugaca uuguguuaga gcauggcggg ugugucaccg ugauggcaca ggauaagcca      720

acaguggaca ucgagcuggu caccacgacu guuucaaaua uggcugaagu gcggaguuac      780

ugcuaugagg ccagcauauc ugauauggcg uccgacucac guugucccac acaaggagaa      840

gccuaccuug acaaacagag cgauacccag uauguaugca agcgcacuuu gguggacaga      900

ggcuggggua acgggugugg acuguucggc aaaggcaguc uagucacaug cgcaaaguuu      960

gcuugcucua agaaaaugac cgggaagucc auucagccug agaaucucga auacaggauc     1020

augcugagcg ugcacggauc gcaacacucu gguaugauug uuaacgauac gggccaugag     1080

accgacgaga aucgggccaa aguggaaauc acaccaaacu caccccgagc ugaggccacu     1140

cuugggggau ucggcucccu ggguuuggau ugugaaccuc gcaccgggcu cgacuuuagu     1200

gaucuguauu acuuaacaau gaauaacaag cacuggcugg uccauaaaga gugguuccac     1260

gacaucccac ucccguggca cgcaggcgcc gauacuggaa caccccauug gaauaacaag     1320

gaagcucuug uggaguuuaa agacgcgcac gcaaagagac agaccguagu ugugcugggc     1380

agccaggagg gggccgucca uaccgcuuug gccggagcac uagaagccga gauggauggu     1440

gcuaagggca ggcuguccuc ugggcaccuc aaaugccggc ugaagaugga caaacuucgc     1500

cugaagggag ugagcuauuc auuguguacu gccgcauuca cguuuacaaa aauuccugcg     1560

gaaacccucc auggcacugu caccguggag guucaguacg cugguacaga cgggccaugc     1620

aaggugcccg cccaaauggc aguagauaug cagacccuga cuccugucgg cagauuaaua     1680

acagccaacc cagugaucac ggaaaguacc gaaaauucca agaugaugcu ggagcuagau     1740

cccccguucg gagacagcua cauuguuauc ggcguggggg agaaaaagau aacucaccac     1800

uggcauaggu cuggaucaac aauugguaag gcuuuugagg ccaccguccg uggcgcaaaa     1860

cggauggcug ugcucgggga cacagccugg gauuucggaa guguaggcgg ugcgcugaac     1920

agccuuggga aaggcaucca ccagaucuuc ggagccgcuu uuaaguccuu guucggcggg     1980

augucuuggu uuucgcaaau ucugaucgga acucuccuga uguggcuugg ccugaauacc     2040

aagaacggua gcauuuccuu gaugugucuc gcauuagggg gagugcugau auuccuguca     2100

acggccguua gugcugacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8308
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8308
gggagaaagc uuaccauggc cagaaaggag aaaaagaggc ggggcgcuga caccagcgug       60

gggaucgucg gacugcuccu uacaacugca auggccgcgg aaguuacgcg ccgagguucc      120

gcuuacuaua uguaccugga ucguaacgac gccggcgagg caauuucuuu ccccaccaca      180

uuggggauga auaaaugcua uauacagauc auggauuuag gacacaugug ugacgccacu      240

augucauacg aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg      300

ugcaacacca caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu      360

agaaggagcc ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccaaacgagg      420

ucgcagacuu ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaauugg      480

aucuuucgaa accccggauu cgcccuggcu gccgcggcaa uugccugguu gcucggcagc      540

aguacauccc agaaagugau cuaucugguc augauacugc ucaucgcucc ugccuacucu      600

auuagaugca ucggcgugag caauagggau uuuguagaag ggauguccgg agguacuugg      660

guugacauug uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca      720

guggacaucg agcuggucac cacgacuguu ucaaacaugg cugaagugcg gaguuacugc      780

uaugaggcca gcauaucuga uauggcgucc gacucacguu gucccacaca aggagaagcc      840

uaccuugaca aacagagcga uacccaguau guaugcaagc gcacuuuggu ggacagaggc      900

ugggguaaug gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu      960

ugcucuaaga aaaugaccgg gaaguccauu cagccugaga accucgaaua caggaucaug     1020

cugagcgugc acggaucgca acacucuggu augauuguua augauacggg ccaugagacc     1080

gacgagaacc gggccaaagu ggaaaucaca ccaaauucac cccgagcuga ggccacucuu     1140

gggggauucg gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau     1200

cuguauuacu uaacaaugaa caauaagcac uggcuggucc auaaagagug guuccacgac     1260

aucccacucc cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa     1320

gcucuugugg aguuuaaaga cgcgcacgca aagagacaga ccguaguugu gcugggcagc     1380

caggaggggg ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu     1440

aagggcaggc uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcauucacgu uuacaaaaau uccugcggaa     1560

acccuccaug gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag     1620

gugcccgccc aaauggcagu agauaugcag acccugacuc cugucggcag auuaauaaca     1680

gccaacccag ugaucacgga aaguaccgag aacuccaaga ugaugcugga gcuagauccc     1740

ccguucggag acagcuacau uguuaucggc gugggggaga aaaagauaac ucaccacugg     1800

cauaggucug gaucaacaau ugguaaggcu uuugaagcca ccguccgugg cgcaaaacgg     1860

auggcugugc ucggggacac agccugggau uucggaagug uaggcggugc gcugaauagc     1920

cuugggaaag gcauccacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug     1980

ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag     2040

aaugguagca uuuccuugau gugucucgca uuagggggag ugcugauauu ccugucaacg     2100

gccguuagug cugacgucgg cugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8309
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8309
gggagaaagc uuaccaugag aaaggagaaa aagaggcggg gcgccgacac cagcgugggg       60

aucgucggac ugcuccuuac aacugcuaug gcagccgaag uuacgcgccg agguuccgcg      120

uacuauaugu accuggaucg uaacgacgcu ggcgaggcca uuucuuuccc caccacauug      180

gggaugaaua aaugcuauau acagaucaug gauuuaggac acauguguga cgcaacuaug      240

ucauacgaau gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc      300

aacaccacaa guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggccaga      360

aggagccggc gcgcugugac acuaccgucc cauucuacca gaaagcucca aacgaggucg      420

cagacuuggc uggagucacg ggaauacacc aagcaccuua uucgcguuga gaauuggauc      480

uuucgaaacc ccggauucgc acuggccgcu gccgcgauug caugguugcu cggcagcagu      540

acaucccaga aagugaucua ucuggucaug auacugcuca ucgccccugc uuacucuauu      600

agaugcaucg gcgugagcaa uagggauuuu guagaaggga uguccggagg uacuuggguu      660

gacauugugu uagagcaugg cggguguguc accgugaugg cccaggauaa gccaacagug      720

gacaucgagc uggucaccac gacuguuuca aacauggcag aagugcggag uuacugcuau      780

gaggcuagca uaucugauau ggccuccgac ucacguuguc ccacacaagg agaagcguac      840

cuugacaaac agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg      900

gguaaugggu guggacuguu cggcaaaggc agucuaguca caugcgccaa guuugcaugc      960

ucuaagaaaa ugaccgggaa guccauucag ccugagaacc ucgaauacag gaucaugcug     1020

agcgugcacg gaucgcaaca cucugguaug auuguuaaug auacgggcca ugagaccgac     1080

gagaaccggg cuaaagugga aaucacacca aauucacccc gagccgaggc uacucuuggg     1140

ggauucggcu cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug     1200

uauuacuuaa caaugaacaa uaagcacugg cugguccaua aagagugguu ccacgacauc     1260

ccacucccgu ggcacgccgg cgcagauacu ggaacacccc auuggaacaa uaaggaagcc     1320

cuuguggagu uuaaagacgc ucacgcgaag agacagaccg uaguugugcu gggcagccag     1380

gagggggcag uccauaccgc cuuggcugga gcccuagaag cagagaugga uggugccaag     1440

ggcaggcugu ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag     1500

ggagugagcu auucauugug uacugcugcc uucacguuua caaaaauucc ugcagaaacc     1560

cuccauggca cugucaccgu ggagguucag uacgcgggua cagacgggcc augcaaggug     1620

cccgcucaaa uggccguaga uaugcagacc cugacuccug ucggcagauu aauaacagca     1680

aacccaguga ucacggaaag uaccgagaac uccaagauga ugcuggagcu agaucccccg     1740

uucggagaca gcuacauugu uaucggcgug ggggagaaaa agauaacuca ccacuggcau     1800

aggucuggau caacaauugg uaaagccuuu gaagcuaccg uccguggcgc caagcggaug     1860

gcagugcucg gggacacagc uugggauuuc ggaaguguag gcggugcccu gaauagccuu     1920

gggaaaggca uccaccagau cuucggagcg gccuuuaagu ccuuguucgg cgggaugucu     1980

ugguuuucgc aaauucugau cggaacucuc cugauguggc uuggccugaa caccaagaau     2040

gguagcauuu ccuugaugug ucucgcuuua gggggagugc ugauauuccu gucaacggca     2100

guuagugccg acgucggcug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8310
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8310
gggagaaagc uuaccaugaa ggagaaaaag agaaggggcg ccgacaccag cguggggauc       60

gucggacugc uccuuacaac ugcuauggca gccgaaguua cgcggcgcgg uuccgcguac      120

uauauguacc uggaucgaaa cgacgcuggc gaggccauuu cuuuccccac cacauugggg      180

augaauaaau gcuauauaca gaucauggau uuaggacaca ugugugacgc aacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcccguaga      360

agcaggcggg cugugacacu accgucccau ucuacccgca agcuccaaac gagaucgcag      420

acuuggcugg agucaaggga auacaccaag caccuuauuc ggguugagaa uuggaucuuu      480

cgcaaccccg gauucgcacu ggccgcugcc gcgauugcau gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugaua cugcucaucg ccccugcuua cucuauucga      600

ugcaucggcg ugagcaauag agauuuugua gaagggaugu ccggagguac uuggguugac      660

auuguguuag agcauggcgg gugugucacc gugauggccc aggauaagcc aacaguggac      720

aucgagcugg ucaccacgac uguuucaaac auggcagaag ugaggaguua cugcuaugag      780

gcuagcauau cugauauggc cuccgacuca cgguguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guauguaugc aagcguacuu ugguggaccg cggcuggggu      900

aaugggugug gacuguucgg caaaggcagu cuagucacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaccucg aauacagaau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauu guuaaugaua cgggccauga gaccgacgag     1080

aacagggcua aaguggaaau cacaccaaau ucaccccggg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagcgc cagaccguag uugugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

agacuguccu cugggcaccu caaaugcagg cugaagaugg acaaacuucg gcugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauuccugc agaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gccgcuuaau aacagcaaac     1680

ccagugauca cggaaaguac cgagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauuguuau cggcgugggg gagaaaaaga uaacucacca cuggcauaga     1800

ucuggaucaa caauugguaa agccuuugaa gcuaccguca ggggcgccaa gcguauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

agcauuuccu ugaugugucu cgcuuuaggg ggagugcuga uauuccuguc aacggcaguu     2100

agugccgacg ucggcugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8311
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8311
gggagaaagc uuaccaugga gaagaaaaga aggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc uuacaacugc uauggcagcc gaaguuacgc ggcgcgguuc cgcguacuau      120

auguaccugg aucgaaacga cgcuggcgag gccauuucuu uccccaccac auuggggaug      180

aauaagugcu auauacagau cauggauuua ggacacaugu gugacgcaac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaaa agggggaggc ccguagaagc      360

aggcgggcug ugacacuacc gucccauucu acccgcaaac uccaaacgag aucgcagacu      420

uggcuggagu caagggaaua caccaagcac cuuauucggg uugagaauug gaucuuucgc      480

aaccccggau ucgcacuggc cgcugccgcg auugcauggu ugcucggcag caguacaucc      540

cagaagguga ucuaucuggu caugauacug cucaucgccc cugcuuacuc uauucgaugc      600

aucggcguga gcaauagaga uuuuguagaa gggauguccg gagguacuug gguugacauu      660

guguuagagc auggcgggug ugucaccgug auggcccagg auaaaccaac aguggacauc      720

gagcugguca ccacgacugu uucaaacaug gcagaaguga ggaguuacug cuaugaggcu      780

agcauaucug auauggccuc cgacucacgg ugucccacac aaggagaagc guaccuugac      840

aagcagagcg auacccagua uguaugcaaa cguacuuugg uggaccgcgg cugggguaau      900

ggguguggac uguucggcaa gggcagucua gucacaugcg ccaaauuugc augcucuaag      960

aagaugaccg ggaaauccau ucagccugag aaccucgaau acagaaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugauuguu aaugauacgg gccaugagac cgacgagaac     1080

agggcuaagg uggaaaucac accaaauuca ccccgggccg aggcuacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucga accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga acaauaaaca cuggcugguc cauaaggagu gguuccacga caucccacuc     1260

ccguggcacg ccggcgcaga uacuggaaca ccccauugga acaauaaaga agcccuugug     1320

gaguuuaagg acgcucacgc gaaacgccag accguaguug ugcugggcag ccaggagggg     1380

gcaguccaua ccgccuuggc uggagcccua gaagcagaga uggauggugc caagggcaga     1440

cuguccucug ggcaccucaa gugcaggcug aaaauggaca agcugcggcu gaaaggagug     1500

agcuauucau uguguacugc ugccuucacg uuuacaaaga uuccugcaga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcg gguacagacg ggccaugcaa agugcccgcu     1620

caaauggccg uagauaugca gacccugacu ccugucggcc gcuuaauaac agcaaaccca     1680

gugaucacgg aaaguaccga gaacuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uuguuaucgg cgugggggaa aagaaaauaa cucaccacug gcauagaucu     1800

ggaucaacaa uugguaaggc cuuugaggcu accgucaggg gcgccaaacg uauggcagug     1860

cucggggaca cagcuuggga uuucggaagu guaggcggug cccugaauag ccuugggaag     1920

ggcauccacc agaucuucgg agcggccuuu aaauccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugaucggaac ucuccugaug uggcuuggcc ugaacaccaa gaaugguagc     2040

auuuccuuga ugugucucgc ucuuggggga guguuaauau uccugucaac ggcaguuagu     2100

gccgacgucg gcugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8312
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8312
gggagaaagc uuaccaugaa gaaaagaagg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuua caacugcuau ggcagccgag guuacgcggc gcgguuccgc guacuauaug      120

uaccuggauc gaaacgacgc uggcgaagcc auuucuuucc ccaccacauu ggggaugaau      180

aagugcuaua uacagaucau ggauuuagga cacaugugug acgcaacuau gucauacgag      240

ugcccuaugc uggaugaagg cguggagcca gacgauguag acuguuggug caacaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaaaaagg gggaagcccg uagaagcagg      360

cgggcuguga cacuaccguc ccauucuacc cgcaaacucc aaacgagauc gcagacuugg      420

cuggagucaa gggaguacac caagcaccuu auucggguug aaaauuggau cuuucgcaac      480

cccggauucg cacuggccgc ugccgcgauu gcaugguugc ucggcagcag uacaucccag      540

aaggugaucu aucuggucau gauacugcuc aucgccccug cuuacucuau ucgaugcauc      600

ggcgugagca auagagauuu uguagagggg auguccggag guacuugggu ugacauugug      660

uuagaacaug gcgggugugu caccgugaug gcccaggaua aaccaacagu ggacaucgag      720

cuggucacca cgacuguuuc aaacauggca gaggugagga guuacugcua ugaagcuagc      780

auaucugaua uggccuccga cucacggugu cccacacaag gagaggcgua ccuugacaag      840

cagagcgaua cccaguaugu augcaaacgu acuuuggugg accgcggcug ggguaauggg      900

uguggacugu ucggcaaggg cagucuaguc acaugcgcca aauuugcaug cucuaagaag      960

augaccggga aauccauuca gccugaaaac cucgaguaca gaaucaugcu gagcgugcac     1020

ggaucgcaac acucugguau gauuguuaau gauacgggcc augaaaccga cgagaacagg     1080

gcuaaggugg agaucacacc aaauucaccc cgggccgaag cuacucuugg gggauucggc     1140

ucccuggguu uggauuguga gccucgaacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgacau cccacucccg     1260

uggcacgccg gcgcagauac uggaacaccc cauuggaaca auaaagaggc ccuuguggaa     1320

uuuaaggacg cucacgcgaa acgccagacc guaguugugc ugggcagcca ggagggggca     1380

guccauaccg ccuuggcugg agcccuagag gcagaaaugg auggugccaa gggcagacug     1440

uccucugggc accucaagug caggcugaaa auggacaagc ugcggcugaa aggagugagc     1500

uauucauugu guacugcugc cuucacguuu acaaagauuc cugcagagac ccuccauggc     1560

acugucaccg uggaaguuca guacgcgggu acagacgggc caugcaaagu gcccgcucaa     1620

auggccguag auaugcagac ccugacuccu gucggccgcu uaauaacagc aaacccagug     1680

aucacggaga guaccgaaaa cuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacauug uuaucggcgu gggggagaag aaaauaacuc accacuggca uagaucugga     1800

ucaacaauug guaaggccuu ugaagcuacc gucaggggcg ccaaacguau ggcagugcuc     1860

ggggacacag cuugggauuu cggaagugua ggcggugccc ugaauagccu ugggaagggc     1920

auccaccaga ucuucggagc ggccuuuaaa uccuuguucg gcgggauguc uugguuuucg     1980

caaauucuga ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu     2040

uccuugaugu gucucgcucu ugggggagug uuaauauucc ugucaacggc aguuagugcc     2100

gacgucggcu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8313
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8313
gggagaaagc uuaccaugaa gagaaggggc gccgacacca gcguggggau cgucggacug       60

cuccuuacaa cugcuauggc agccgagguu acgcggcgcg guuccgcgua cuauauguac      120

cuggaucgaa acgacgcugg cgaagccauu ucuuucccca ccacauuggg gaugaauaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg caacuauguc auacgagugc      240

ccuaugcugg augaaggcgu ggagccagac gauguagacu guuggugcaa caccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aagcccguag aagcaggcgg      360

gcugugacac uaccguccca uucuacccgc aagcuccaaa cgagaucgca gacuuggcug      420

gagucaaggg aguacaccaa acaccuuauu cggguugaaa auuggaucuu ucgcaacccc      480

ggauucgcac uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaag      540

gugaucuauc uggucaugau acugcucauc gccccugcuu acucuauucg augcaucggc      600

gugagcaaua gagauuuugu agaggggaug uccggaggua cuuggguuga cauuguguua      660

gaacauggcg ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa cauggcagag gugaggaguu acugcuauga agcuagcaua      780

ucugauaugg ccuccgacuc acgguguccc acacaaggag aggcguaccu ugacaaacag      840

agcgauaccc aguauguaug caagcguacu uugguggacc gcggcugggg uaaugggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaaaaagaug      960

accgggaagu ccauucagcc ugaaaaccuc gaguacagaa ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaaugau acgggccaug aaaccgacga gaacagggcu     1080

aaaguggaga ucacaccaaa uucaccccgg gccgaagcua cucuuggggg auucggcucc     1140

cuggguuugg auugugagcc ucgaaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaacaaua agcacuggcu gguccauaaa gaaugguucc acgacauccc acucccgugg     1260

cacgccggcg cagauacugg aacaccccau uggaacaaua aggaggcccu uguggaauuu     1320

aaagacgcuc acgcgaagcg ccagaccgua guugugcugg gcagccagga gggggcaguc     1380

cauaccgccu uggcuggagc ccuagaggca gaaauggaug gugccaaagg cagacugucc     1440

ucugggcacc ucaagugcag gcugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucauugugua cugcugccuu cacguuuaca aaaauuccug cagagacccu ccauggcacu     1560

gucaccgugg aaguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug     1620

gccguagaua ugcagacccu gacuccuguc ggccgcuuaa uaacagcaaa cccagugauc     1680

acggagagua ccgaaaacuc caaaaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaagaag auaacucacc acuggcauag aucuggauca     1800

acaauuggua aagccuuuga agcuaccguc aggggcgcca agcguauggc agugcucggg     1860

gacacagcuu gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc     1920

caccagaucu ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaca ccaaaaaugg uagcauuucc     2040

uugauguguc ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac     2100

gucggcugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8314
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8314
gggagaaagc uuaccaugag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug cuauggcagc cgagguuacg cggcgcgguu ccgcguacua uauguaccug      120

gaucgaaacg acgcuggcga agccauuucu uuccccacca cauuggggau gaauaagugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgagugcccu      240

augcuggaug aaggcgugga gccagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaaa aagggggaag cccguagaag caggcgggcu      360

gugacacuac cgucccauuc uacccgcaaa cuccaaacga gaucgcagac uuggcuggag      420

ucaagggagu acaccaagca ccuuauucgg guugaaaauu ggaucuuucg caaccccgga      480

uucgcacugg ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug      600

agcaauagag auuuuguaga ggggaugucc ggagguacuu ggguugacau uguguuagaa      660

cauggcgggu gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcagaggug aggaguuacu gcuaugaagc uagcauaucu      780

gauauggccu ccgacucacg gugucccaca caaggagagg cguaccuuga caagcagagc      840

gauacccagu auguaugcaa acguacuuug guggaccgcg gcugggguaa ugggugugga      900

cuguucggca agggcagucu agucacaugc gccaaauuug caugcucuaa gaaaaugacc      960

gggaagucca uucagccuga aaaccucgag uacagaauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaaa ccgacgagaa cagggcuaag     1080

guggagauca caccaaauuc accccgggcc gaagcuacuc uugggggauu cggcucccug     1140

gguuuggauu gugagccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu ccauaaggaa ugguuccacg acaucccacu cccguggcac     1260

gccggcgcag auacuggaac accccauugg aacaauaaag aggcccuugu ggaauuuaag     1320

gacgcucacg cgaaacgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau     1380

accgccuugg cuggagcccu agaggcagaa auggauggug ccaagggcag acuguccucu     1440

gggcaccuca aaugcaggcu gaagauggac aagcugcggc ugaaaggagu gagcuauuca     1500

uuguguacug cugccuucac guuuacaaag auuccugcag agacccucca uggcacuguc     1560

accguggaag uucaguacgc ggguacagac gggccaugca aagugcccgc ucaaauggcc     1620

guagauaugc agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca     1800

auugguaagg ccuuugaagc uaccgucagg ggcgccaaac guauggcagu gcucggggac     1860

acagcuuggg auuucggaag uguaggcggu gcccugaaua gccuugggaa gggcauccac     1920

cagaucuucg gagcggccuu uaaauccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cucuuggggg aguguuaaua uuccugucaa cggcaguuag ugccgacguc     2100

ggcugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8315
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8315
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga gguuacgagg cgggguuccg cguacuauau guaccuggau      120

cgcaacgacg cuggcgaagc cauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga gugcccuaug      240

cuggaugaag gcguggagcc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaagccc gacguagcag aagggcugug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaguca      420

agagaguaca ccaagcaccu uauuaggguu gaaaauugga ucuuucggaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaggg gauguccgga gguacuuggg uugacauugu guuagaacau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaggugaga aguuacugcu augaagcuag cauaucugau      780

auggccuccg acucaaggug ucccacacaa ggagaggcgu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugaaaa ccucgaguac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugaaaccg acgagaacag agcuaaggug     1080

gagaucacac caaauucccc cagggccgaa gcuacucuug ggggauucgg cucacugggu     1140

uuggauugug agccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggaaugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaagagg cccuugugga auuuaaggac     1320

gcucacgcga aacgacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga ggcagaaaug gauggugcca agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaagauu ccugcagaga cccuccaugg cacugucacc     1560

guggaaguuc aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag caaacccagu gaucacggag     1680

aguaccgaaa acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggccu uugaagcuac cgucagaggc gccaaaagga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuc uugggggagu guuaauauuc cugucaacgg caguuagugc cgacgucggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8316
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8316
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaggu uacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

aacgacgcug gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug      240

gaugaaggcg uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caaaaagggg gaagcccgcc gaagccguag agcugugaca      360

cuaccguccc auucuaccag gaaacuccaa acgcggucgc agacuuggcu ggagucacgc      420

gaguacacca agcaccuuau uagaguugaa aauuggaucu uuaggaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc ggugcaucgg cgugagcaau      600

cgcgauuuug uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga ggugcgaagu uacugcuaug aagcuagcau aucugauaug      780

gccuccgacu caagaugucc cacacaagga gaggcguacc uugacaagca gagcgauacc      840

caguauguau gcaaaaggac uuugguggac cggggcuggg guaaugggug uggacuguuc      900

ggcaagggca gucuagucac augcgccaaa uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugaaaaccu cgaguaccgu aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gaaaccgacg agaaccgcgc uaagguggag     1080

aucacaccaa auucccccag agccgaagcu acucuugggg gauucggcuc acuggguuug     1140

gauugugagc cuaggaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aaacacuggc ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu     1320

cacgcgaaac ggcagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaggc agaaauggau ggugccaagg gccgacuguc cucugggcac     1440

cucaaaugcc gccugaagau ggacaagcug agacugaaag gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug     1560

gaaguucagu acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggcagguua auaacagcaa acccagugau cacggagagu     1680

accgaaaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcauc ggucuggauc aacaauuggu     1800

aaggccuuug aagcuaccgu ccgcggcgcc aaaagaaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc     1920

uucggagcgg ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcucuug ggggaguguu aauauuccug ucaacggcag uuagugccga cgucggcugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8317
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8317
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccuuacaacu       60

gcuauggcag ccgagguuac gagaagggga uccgcguacu auauguaccu ggaucggaac      120

gacgcuggug aagccauuuc uuuccccacc acauugggca ugaauaagug cuauauacag      180

aucauggauu uagggcacau gugugacgca acuaugucau acgagugccc uaugcuggau      240

gaaggagugg agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uauggcacuu gucaucacaa aaagggugaa gcccgccgaa gccguagagc ugugacacua      360

ccgucccauu cuaccaggaa acuccaaacg cggucgcaga cuuggcugga gucacgcgag      420

uacaccaagc accuuauuag aguugaaaau uggaucuuua ggaaccccgg guucgcacug      480

gccgcugccg cgauugcaug guugcucgga agcaguacau cccagaaagu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucggu gcaucggcgu gagcaaucgc      600

gauuuuguag agggcauguc cgggggaacu uggguugaca uuguguuaga acaugguggc      660

ugugucaccg ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaggu gcgaaguuac ugcuaugaag cuagcauauc ugauauggcc      780

uccgacucaa gaugucccac acaaggggag gcguaccuug acaagcagag cgauacccag      840

uauguaugca aaaggacuuu gguggaccgg ggauggggca augguugugg gcuguucgga      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaaaaugac cggcaagucc      960

auucagccug aaaaccucga guaccguauc augcugagcg ugcacggguc gcaacacucu     1020

ggaaugauug uuaaugauac gggucaugaa accgacgaga accgcgcuaa gguggagauc     1080

acaccaaauu cccccagagc cgaagcuacu cuuggcgggu ucggaucacu gggcuuggau     1140

ugugagccua ggaccggucu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gacaucccac ucccguggca cgccggggca     1260

gauacuggca caccccauug gaacaauaaa gaggcccuug uggaauuuaa ggacgcucac     1320

gcgaaacggc agaccguagu ugugcuggga agccaggagg gcgcagucca uaccgccuug     1380

gcuggggccc uagaggcaga aauggaugga gccaaggguc gacuguccuc uggccaccuc     1440

aaaugccgcc ugaagaugga caaacuuaga cugaaggggg ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gagacccucc auggaacugu caccguggaa     1560

guucaguacg cgggcacaga cgguccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg gagguuaaua acagcaaacc cagugaucac ggagaguacc     1680

gaaaacucca agaugaugcu ggagcuagau cccccguucg gcgacagcua cauuguuauc     1740

ggagugggcg agaaaaagau aacucaccac uggcaucggu cugggucaac aauuggaaag     1800

gccuuugaag cuaccguccg cggugccaaa agaauggcag ugcucggcga cacagcuugg     1860

gauuucggga guguaggagg cgcccugaau agccuuggua aggggaucca ccagaucuuc     1920

ggcgcggccu uuaaauccuu guucggaggc augucuuggu uuucgcaaau ucugaucggg     1980

acucuccuga uguggcuugg acugaacacc aagaauggca gcauuuccuu gaugugucuc     2040

gcucugggug ggguguuaau auuccuguca acggcaguua gugccgacgu cggaugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8318
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8318
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu uacaacugcc       60

auggcugcag agguuacgag aaggggaucc gccuacuaua uguaccugga ucggaacgac      120

gcgggugaag cuauuucuuu ccccaccaca uugggcauga auaagugcua uauacagauc      180

auggauuuag ggcacaugug ugacgccacu augucauacg agugcccuau gcuggaugaa      240

ggaguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

ggcacuuguc aucacaaaaa gggugaagca cgccgaagcc guagagccgu gacacuaccg      360

ucccauucua ccaggaaacu ccaaacgcgg ucgcagacuu ggcuggaguc acgcgaguac      420

accaagcacc uuauuagagu ugaaaauugg aucuuuagga accccggguu cgcucuggca      480

gccgcugcca uugcgugguu gcucggaagc aguacauccc agaaagugau cuaucugguc      540

augauacugc ucaucgcacc cgccuacucu auucggugca ucggcgugag caaucgcgau      600

uuuguagagg gcauguccgg gggaacuugg guugacauug uguuagaaca ugguggcugu      660

gucaccguga uggcucagga uaagccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg ccgaggugcg aaguuacugc uaugaagcaa gcauaucuga uauggcuucc      780

gacucaagau gucccacaca aggggaggcc uaccuugaca agcagagcga uacccaguau      840

guaugcaaaa ggacuuuggu ggaccgggga uggggcaaug guugugggcu guucggaaag      900

ggcagucuag ucacaugcgc gaaauuugcc ugcucuaaga aaaugaccgg caaguccauu      960

cagccugaaa accucgagua ccguaucaug cugagcgugc acgggucgca acacucugga     1020

augauuguua augauacggg ucaugaaacc gacgagaacc gcgcaaaggu ggagaucaca     1080

ccaaauuccc ccagagcuga agccacucuu ggcggguucg gaucacuggg cuuggauugu     1140

gagccuagga ccggucucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc uggggccgau     1260

acuggcaccc cucauuggaa caauaaagag gcacuugugg aauuuaagga cgcccacgcu     1320

aaacggcaga caguaguugu gcugggaagc caggagggcg cgguccauac cgcauuggcc     1380

ggggcucuag aggccgaaau ggauggagca aagggucgac uguccucugg ccaccucaaa     1440

ugccgccuga agauggacaa acuuagacug aaggggguga gcuauucauu guguacugcc     1500

gcuuucacgu uuacaaagau uccugccgag acccuccaug gaacugucac cguggaaguu     1560

caguacgcag gcacagacgg uccaugcaaa gugcccgcgc aaauggcugu agauaugcag     1620

acccugacuc cugucgggag guuaauaaca gccaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggcg acagcuacau uguuaucgga     1740

gugggcgaga aaaagauaac ucaccacugg caucggucug ggucaacaau uggaaaggca     1800

uuugaagcca ccguccgcgg ugcuaaaaga auggccgugc ucggcgacac agcaugggau     1860

uucgggagug uaggaggcgc ucugaauagc cuugguaagg ggauccacca gaucuucggc     1920

gccgcguuua aauccuuguu cggaggcaug ucuugguuuu cgcaaauucu gaucgggacu     1980

cuccugaugu ggcuuggacu gaacaccaag aauggcagca uuuccuugau gugucucgcc     2040

cugggugggg uguuaauauu ccugucaacg gcuguuagug cagacgucgg augcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8319
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8319
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcag augucggaug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8320
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8320
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagccg uuacgagaag gggauccgcg uacuauaugu accuggaccg gaacgaugcu      120

ggugaggcca uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcaacuaug ucauacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaggcccgc cgaagccgua gagcugugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggaaucacg cgaguacacc      420

aagcaccuua uuagaguuga gaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagaaggca uguccggggg aacuuggguu gauauugugu uagagcaugg uggcuguguc      660

accgugaugg cccaggacaa gccaacagug gauaucgaac uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaggcuagca uaucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaagcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugagaacc ucgaauaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugagaccgau gaaaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaggc uacucuuggc ggguucggau cacugggcuu ggacugugaa     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggagugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacacu     1260

ggcacacccc auuggaacaa uaaagaagcc cuuguggagu uuaaggaugc ucacgcgaaa     1320

cggcagaccg uaguugugcu gggaagccag gaaggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagagaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagaaacc cuccauggaa cugucaccgu ggagguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaggcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccg augucggaug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8321
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8321
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccgc gguuacgagg cgggguuccg cuuacuauau guaccuggau      120

cgcaacgacg ccggcgaggc aauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgccacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaggcuc gacguagcag aagggcagug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaauca      420

agagaguaca ccaagcaccu uauuaggguu gagaauugga ucuuucggaa ccccggauuc      480

gcccuggcug ccgcggcaau ugccugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgcuccu gccuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcacaggau aagccaacag uggacaucga acuggucacc      720

acgacuguuu caaacauggc ugaggugaga aguuacugcu augaggccag cauaucugau      780

auggcguccg acucaaggug ucccacacaa ggagaagccu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgca aaauuugcuu gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgaaaacag agccaaggug     1080

gagaucacac caaauucccc cagggcugag gccacucuug ggggauucgg cucacugggu     1140

uuggauugug aaccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgca     1260

ggcgccgaua cuggaacacc ccauuggaac aauaaagaag cucuugugga guuuaaggac     1320

gcgcacgcaa aacgacagac cguaguugug cugggcagcc aggaaggggc cguccauacc     1380

gcuuuggccg gagcacuaga ggccgagaug gauggugcua agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugccg cauucacguu uacaaagauu ccugcggaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcugg uacagacggg ccaugcaaag ugcccgccca aauggcagua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag ccaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggaa cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggcuu uugaggccac cgucagaggc gcaaaaagga uggcugugcu cggggacaca     1860

gccugggauu ucggaagugu aggcggugcg cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag ccgcuuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcac uugggggagu guuaauauuc cugucaacgg ccguuagugc ugacgucggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8322
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8322
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagguguuac ucgacguggc      120

ucagccuacu auauguaccu ggauagaaac gacgcugggg aggcaauuag uuuucccacg      180

acccuaggaa ugaauaaaug cuauauacaa aucauggauc ucggccauau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggugugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugggaccu gucaccauaa gaaaggagag      360

gcgaggcggu cucgcagagc ugugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg cuuugcccug gcagccgcug caauugccug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc uccugccuac      600

uccauuaggu gcaucggggu gucaaaucgg gauuucguag aaggaaugag ugguggcaca      660

uggguugaca uuguguugga gcacggggga ugugucacug ugauggcgca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggcagaggu gcguucuuac      780

ugcuaugagg ccuccauauc agauauggcu agcgacaguc gcugucccac ucagggcgaa      840

gccuaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

gguuggggga auggaugugg ccuguuuggc aaaggguccc uugucacuug cgcaaaguuc      960

gcuugcagca aaaagaugac aggaaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacgguuc ccagcauuca ggcaugauug uuaaugauac cgggcacgag     1080

acggacgaaa accgagccaa aguggagauc accccaaaua guccccgcgc ggaggccaca     1140

cucggaggcu uugguagccu gggguuagau ugugaaccua gaacuggccu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcaggagcu gauacaggca cuccccacug gaacaauaag     1320

gaagcccuag uggaguucaa agacgcucau gccaagaggc agaccguagu ugugcugggg     1380

ucucaggaag gagcagucca cacagcccuc gcuggugcgc uggaggcaga gauggauggc     1440

gccaaagggc ggcuuagcuc aggacaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggcg ugaguuauuc cuuauguacc gcugccuuua cuuucacgaa aauuccugca     1560

gaaacacugc acgguaccgu cacuguggag guucaauacg ccgggaccga cggcccaugc     1620

aaggugcccg cucagauggc cguagauaug cagacacuaa ccccugucgg aaggcucaua     1680

acugcaaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gcgacucaua cauuguuauc ggggugggag agaagaagau aacccaccau     1800

uggcaccgua gugguagcac uauuggcaaa gcguucgagg cuacaguccg aggggccaag     1860

cggauggcag uguugggaga caccgccugg gauuucggcu ccguaggugg ggcucucaau     1920

ucucugggca aaggaaucca ucaaaucuuu ggcgccgcau ucaagucgcu guuuggggga     1980

augagcuggu ucucccagau ucuuaucggc acacuguuga uguggcucgg ucugaacacu     2040

aaaaaugggu caauuaguuu aaugugucug gcucuaggag gcgugcucau auuucugucu     2100

accgccguua gcgcggacgu cgguugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8323
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8323
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagccguuac ucgacguggu      120

ucagcuuacu auauguaccu ggauagaaac gacgcaggcg aggccauuag uuuucccacg      180

acccuaggga ugaauaaaug cuauauacaa aucauggauc ucggacauau gugugacgcg      240

acaaugagcu acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugguaccu gucaccauaa gaaaggggag      360

gcuaggcggu cucgcagagc cgugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg auuugcacug gccgcugcag ccauugcuug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc cccugcguac      600

uccauuaggu gcaucggcgu gucaaaucgg gauuucguag aagggaugag uggagguaca      660

uggguugaca uuguguugga gcacggcggg ugugucacug ugauggcaca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggccgaggu gcguucuuac      780

ugcuaugagg cuuccauauc agauauggcc agcgacaguc gcugucccac ucagggagaa      840

gcauaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

ggcuggggua augggugugg acuguuuggc aaaggcuccc uugucacuug cgcuaaguuc      960

gccugcagca aaaagaugac agggaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacggauc ccagcauuca gguaugauug uuaaugauac cggccacgag     1080

acggacgaaa accgagcgaa aguggagauc accccaaaua guccccgcgc cgaggcaaca     1140

cucgggggau uuggcagccu ggguuuagau ugugaaccua gaacugggcu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcuggcgcc gauacaggaa cuccccacug gaacaauaag     1320

gaagcucuag uggaguucaa agacgcccau gcaaagaggc agaccguagu ugugcugggc     1380

ucucaggaag gggccgucca cacagcucuc gcgggagcac uggaggccga gauggauggu     1440

gcuaaaggcc ggcuuagcuc agggcaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggag ugaguuauuc cuuauguacc gccgcauuua cuuucacgaa aauuccugcc     1560

gaaacacugc acggcaccgu cacuguggag guucaauacg cugguaccga cgggccaugc     1620

aaggugcccg cccagauggc aguagauaug cagacacuaa ccccugucgg caggcucaua     1680

acugcgaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gagacucaua cauuguuauc ggcguggggg agaagaagau aacccaccau     1800

uggcaccgua guggaagcac uauugguaag gcuuucgagg ccacaguccg gggcgcaaaa     1860

cgaauggccg uguuggggga caccgcuugg gauuucggau ccguaggcgg ugcccucaau     1920

ucucugggga aaggcaucca ucaaaucuuu ggagcagcuu ucaagucgcu guuuggcggg     1980

augagcuggu ucucccagau ucuuaucgga acacuguuga uguggcucgg ccugaacacu     2040

aaaaaugguu caauuaguuu aaugugucug gcccuagggg gagugcucau auuucugucu     2100

accgcgguua gcgccgacgu cggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8324
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8324
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8325
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8325
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagaggcg gggcgcugac       60

accagcgugg ggauagucgg acugcuccuu acaacugcaa uggccgcgga aguuacgcgc      120

cgagguuccg cuuacuauau guaccuggau cguaaugacg ccggcgaggc aaucucuuuc      180

cccaccacau uggggaugaa caaaugcuau auucagauca uggauuuagg acacaugugu      240

gacgccacua ugucauacga augcccuaug cuggaugagg gcguggaacc agacgaugua      300

gacuguuggu gcaauaccac aaguaccugg guggucuaug guacuuguca ucacaagaaa      360

ggggaggcua gaaggagccg gcgcgcagug acacuaccgu cccauucuac cagaaagcuc      420

caaacgaggu cgcagacuug gcuggaguca cgggaauaca ccaagcaccu uauucgcguu      480

gagaacugga ucuuucgaaa ucccggauuc gcccuggcug ccgcggcaau agccugguug      540

cucggcagca guacauccca gaaagugauc uaucugguca ugauucugcu caucgcuccu      600

gccuacucua uuagaugcau cggcgugagc aacagggauu uuguagaagg gauguccgga      660

gguacuuggg uugacauagu guuagagcau ggcgggugug ucaccgugau ggcacaggau      720

aagccaacag uggacauuga gcuggucacc acgacuguuu caaauauggc ugaagugcgg      780

aguuacugcu augaggccag caucucugau auggcguccg acucacguug ucccacacaa      840

ggagaagccu accuugacaa acagagcgau acccaguaug uaugcaagcg cacuuuggug      900

gacagaggcu gggguaacgg guguggacug uucggcaaag gcagucuagu cacaugcgca      960

aaguuugcuu gcucuaagaa aaugaccggg aaguccauuc agccugagaa ucucgaauac     1020

aggaucaugc ugagcgugca cggaucgcaa cacucuggua ugaucguuaa cgauacgggc     1080

caugagaccg acgagaaucg ggccaaagug gaaauuacac caaacucacc ccgagcugag     1140

gccacucuug ggggauucgg cucccugggu uuggauugug aaccucgcac cgggcucgac     1200

uuuagugauc uguauuacuu aacaaugaau aacaagcacu ggcuggucca uaaagagugg     1260

uuccacgaca uaccacuccc guggcacgca ggcgccgaua cuggaacacc ccauuggaau     1320

aacaaggaag cucuugugga guuuaaagac gcgcacgcaa agagacagac cguaguugug     1380

cugggcagcc aggagggggc cguccauacc gcuuuggccg gagcacuaga agccgagaug     1440

gauggugcua agggcaggcu guccucuggg caccucaaau gccggcugaa gauggacaaa     1500

cuucgccuga agggagugag cuauucauug uguacugccg cauucacguu uacaaaaauc     1560

ccugcggaaa cccuccaugg cacugucacc guggagguuc aguacgcugg uacagacggg     1620

ccaugcaagg ugcccgccca aauggcagua gauaugcaga cccugacucc ugucggcaga     1680

uuaauuacag ccaacccagu gaucacggaa aguaccgaaa auuccaagau gaugcuggag     1740

cuagaucccc cguucggaga cagcuacaua guuauuggcg ugggggagaa aaagaucacu     1800

caccacuggc auaggucugg aucaacaauc gguaaggcuu uugaggccac cguccguggc     1860

gcaaaacgga uggcugugcu cggggacaca gccugggauu ucggaagugu aggcggugcg     1920

cugaacagcc uugggaaagg cauucaccag aucuucggag ccgcuuuuaa guccuuguuc     1980

ggcgggaugu cuugguuuuc gcaaauucug auaggaacuc uccugaugug gcuuggccug     2040

aauaccaaga acgguagcau cuccuugaug ugucucgcau uagggggagu gcugauuuuc     2100

cugucaacgg ccguuagugc ugacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8326
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8326
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agaggcgggg cgcugacacc       60

agcgugggga uugucggacu gcuccuuaca acugcaaugg ccgcggaagu uacgcgccga      120

gguuccgcuu acuauaugua ccuggaucgu aaugacgccg gcgaggcaau aucuuucccc      180

accacauugg ggaugaacaa augcuauauc cagauuaugg auuuaggaca caugugugac      240

gccacuaugu cauacgaaug cccuaugcug gaugagggcg uggaaccaga cgauguagac      300

uguuggugca auaccacaag uaccugggug gucuauggua cuugucauca caagaaaggg      360

gaggcuagaa ggagccggcg cgcagugaca cuaccguccc auucuaccag aaagcuccaa      420

acgaggucgc agacuuggcu ggagucacgg gaauacacca agcaccuuau ccgcguugag      480

aacuggauuu uucgaaaucc cggauucgcc cuggcugccg cggcaaucgc cugguugcuc      540

ggcagcagua caucccagaa agugauauau cuggucauga uccugcucau ugcuccugcc      600

uacucuauca gaugcauugg cgugagcaac agggauuuug uagaagggau guccggaggu      660

acuuggguug acaucguguu agagcauggc ggguguguca ccgugauggc acaggauaag      720

ccaacagugg acauagagcu ggucaccacg acuguuucaa auauggcuga agugcggagu      780

uacugcuaug aggccagcau uucugauaug gcguccgacu cacguugucc cacacaagga      840

gaagccuacc uugacaaaca gagcgauacc caguauguau gcaagcgcac uuugguggac      900

agaggcuggg guaacgggug uggacuguuc ggcaaaggca gucuagucac augcgcaaag      960

uuugcuugcu cuaagaaaau gaccgggaag uccauccagc cugagaaucu cgaauacagg     1020

auuaugcuga gcgugcacgg aucgcaacac ucugguauga ucguuaacga uacgggccau     1080

gagaccgacg agaaucgggc caaaguggaa aucacaccaa acucaccccg agcugaggcc     1140

acucuugggg gauucggcuc ccuggguuug gauugugaac cucgcaccgg gcucgacuuu     1200

agugaucugu auuacuuaac aaugaauaac aagcacuggc ugguccauaa agagugguuc     1260

cacgacauuc cacucccgug gcacgcaggc gccgauacug gaacacccca uuggaauaac     1320

aaggaagcuc uuguggaguu uaaagacgcg cacgcaaaga gacagaccgu aguugugcug     1380

ggcagccagg agggggccgu ccauaccgcu uuggccggag cacuagaagc cgagauggau     1440

ggugcuaagg gcaggcuguc cucugggcac cucaaaugcc ggcugaagau ggacaaacuu     1500

cgccugaagg gagugagcua uucauugugu acugccgcau ucacguuuac aaaaauaccu     1560

gcggaaaccc uccauggcac ugucaccgug gagguucagu acgcugguac agacgggcca     1620

ugcaaggugc ccgcccaaau ggcaguagau augcagaccc ugacuccugu cggcagauua     1680

aucacagcca acccagugau uacggaaagu accgaaaauu ccaagaugau gcuggagcua     1740

gaucccccgu ucggagacag cuacaucguu auaggcgugg gggagaaaaa gauuacucac     1800

cacuggcaua ggucuggauc aacaaucggu aaggcuuuug aggccaccgu ccguggcgca     1860

aaacggaugg cugugcucgg ggacacagcc ugggauuucg gaaguguagg cggugcgcug     1920

aacagccuug ggaaaggcau ccaccagauu uucggagccg cuuuuaaguc cuuguucggc     1980

gggaugucuu gguuuucgca aauccugauu ggaacucucc ugauguggcu uggccugaau     2040

accaagaacg guagcauauc cuugaugugu cucgcauuag ggggagugcu gaucuuccug     2100

ucaacggccg uuagugcuga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8327
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8327
gggagaaagc uuaccaugaa cgccagaaag gagaaaaaga ggcggggcgc ugacaccagc       60

guggggaucg ucggacugcu ccuuacaacu gcaauggccg cggaaguuac gcgccgaggu      120

uccgcuuacu auauguaccu ggaucguaau gacgccggcg aggcaauuuc uuuccccacc      180

acauugggga ugaacaaaug cuauauacag aucauggauu uaggacacau gugugacgcc      240

acuaugucau acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaua ccacaaguac cugggugguc uaugguacuu gucaucacaa gaaaggggag      360

gcuagaagga gccggcgcgc agugacacua ccgucccauu cuaccagaaa gcuccaaacg      420

aggucgcaga cuuggcugga gucacgggaa uacaccaagc accuuauucg cguugagaac      480

uggaucuuuc gaaaucccgg auucgcccug gcugccgcgg caauugccug guugcucggc      540

agcaguacau cccagaaagu gaucuaucug gucaugauac ugcucaucgc uccugccuac      600

ucuauuagau gcaucggcgu gagcaacagg gauuuuguag aagggauguc cggagguacu      660

uggguugaca uuguguuaga gcauggcggg ugugucaccg ugauggcaca ggauaagcca      720

acaguggaca ucgagcuggu caccacgacu guuucaaaua uggcugaagu gcggaguuac      780

ugcuaugagg ccagcauauc ugauauggcg uccgacucac guugucccac acaaggagaa      840

gccuaccuug acaaacagag cgauacccag uauguaugca agcgcacuuu gguggacaga      900

ggcuggggua acgggugugg acuguucggc aaaggcaguc uagucacaug cgcaaaguuu      960

gcuugcucua agaaaaugac cgggaagucc auucagccug agaaucucga auacaggauc     1020

augcugagcg ugcacggauc gcaacacucu gguaugauug uuaacgauac gggccaugag     1080

accgacgaga aucgggccaa aguggaaauc acaccaaacu caccccgagc ugaggccacu     1140

cuugggggau ucggcucccu ggguuuggau ugugaaccuc gcaccgggcu cgacuuuagu     1200

gaucuguauu acuuaacaau gaauaacaag cacuggcugg uccauaaaga gugguuccac     1260

gacaucccac ucccguggca cgcaggcgcc gauacuggaa caccccauug gaauaacaag     1320

gaagcucuug uggaguuuaa agacgcgcac gcaaagagac agaccguagu ugugcugggc     1380

agccaggagg gggccgucca uaccgcuuug gccggagcac uagaagccga gauggauggu     1440

gcuaagggca ggcuguccuc ugggcaccuc aaaugccggc ugaagaugga caaacuucgc     1500

cugaagggag ugagcuauuc auuguguacu gccgcauuca cguuuacaaa aauuccugcg     1560

gaaacccucc auggcacugu caccguggag guucaguacg cugguacaga cgggccaugc     1620

aaggugcccg cccaaauggc aguagauaug cagacccuga cuccugucgg cagauuaaua     1680

acagccaacc cagugaucac ggaaaguacc gaaaauucca agaugaugcu ggagcuagau     1740

cccccguucg gagacagcua cauuguuauc ggcguggggg agaaaaagau aacucaccac     1800

uggcauaggu cuggaucaac aauugguaag gcuuuugagg ccaccguccg uggcgcaaaa     1860

cggauggcug ugcucgggga cacagccugg gauuucggaa guguaggcgg ugcgcugaac     1920

agccuuggga aaggcaucca ccagaucuuc ggagccgcuu uuaaguccuu guucggcggg     1980

augucuuggu uuucgcaaau ucugaucgga acucuccuga uguggcuugg ccugaauacc     2040

aagaacggua gcauuuccuu gaugugucuc gcauuagggg gagugcugau auuccuguca     2100

acggccguua gugcugacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8328
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8328
gggagaaagc uuaccauggc cagaaaggag aaaaagaggc ggggcgcuga caccagcgug       60

gggaucgucg gacugcuccu uacaacugca auggccgcgg aaguuacgcg ccgagguucc      120

gcuuacuaua uguaccugga ucguaacgac gccggcgagg caauuucuuu ccccaccaca      180

uuggggauga auaaaugcua uauacagauc auggauuuag gacacaugug ugacgccacu      240

augucauacg aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg      300

ugcaacacca caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu      360

agaaggagcc ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccaaacgagg      420

ucgcagacuu ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaauugg      480

aucuuucgaa accccggauu cgcccuggcu gccgcggcaa uugccugguu gcucggcagc      540

aguacauccc agaaagugau cuaucugguc augauacugc ucaucgcucc ugccuacucu      600

auuagaugca ucggcgugag caauagggau uuuguagaag ggauguccgg agguacuugg      660

guugacauug uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca      720

guggacaucg agcuggucac cacgacuguu ucaaacaugg cugaagugcg gaguuacugc      780

uaugaggcca gcauaucuga uauggcgucc gacucacguu gucccacaca aggagaagcc      840

uaccuugaca aacagagcga uacccaguau guaugcaagc gcacuuuggu ggacagaggc      900

ugggguaaug gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu      960

ugcucuaaga aaaugaccgg gaaguccauu cagccugaga accucgaaua caggaucaug     1020

cugagcgugc acggaucgca acacucuggu augauuguua augauacggg ccaugagacc     1080

gacgagaacc gggccaaagu ggaaaucaca ccaaauucac cccgagcuga ggccacucuu     1140

gggggauucg gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau     1200

cuguauuacu uaacaaugaa caauaagcac uggcuggucc auaaagagug guuccacgac     1260

aucccacucc cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa     1320

gcucuugugg aguuuaaaga cgcgcacgca aagagacaga ccguaguugu gcugggcagc     1380

caggaggggg ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu     1440

aagggcaggc uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcauucacgu uuacaaaaau uccugcggaa     1560

acccuccaug gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag     1620

gugcccgccc aaauggcagu agauaugcag acccugacuc cugucggcag auuaauaaca     1680

gccaacccag ugaucacgga aaguaccgag aacuccaaga ugaugcugga gcuagauccc     1740

ccguucggag acagcuacau uguuaucggc gugggggaga aaaagauaac ucaccacugg     1800

cauaggucug gaucaacaau ugguaaggcu uuugaagcca ccguccgugg cgcaaaacgg     1860

auggcugugc ucggggacac agccugggau uucggaagug uaggcggugc gcugaauagc     1920

cuugggaaag gcauccacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug     1980

ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag     2040

aaugguagca uuuccuugau gugucucgca uuagggggag ugcugauauu ccugucaacg     2100

gccguuagug cugacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8329
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8329
gggagaaagc uuaccaugag aaaggagaaa aagaggcggg gcgccgacac cagcgugggg       60

aucgucggac ugcuccuuac aacugcuaug gcagccgaag uuacgcgccg agguuccgcg      120

uacuauaugu accuggaucg uaacgacgcu ggcgaggcca uuucuuuccc caccacauug      180

gggaugaaua aaugcuauau acagaucaug gauuuaggac acauguguga cgcaacuaug      240

ucauacgaau gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc      300

aacaccacaa guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggccaga      360

aggagccggc gcgcugugac acuaccgucc cauucuacca gaaagcucca aacgaggucg      420

cagacuuggc uggagucacg ggaauacacc aagcaccuua uucgcguuga gaauuggauc      480

uuucgaaacc ccggauucgc acuggccgcu gccgcgauug caugguugcu cggcagcagu      540

acaucccaga aagugaucua ucuggucaug auacugcuca ucgccccugc uuacucuauu      600

agaugcaucg gcgugagcaa uagggauuuu guagaaggga uguccggagg uacuuggguu      660

gacauugugu uagagcaugg cggguguguc accgugaugg cccaggauaa gccaacagug      720

gacaucgagc uggucaccac gacuguuuca aacauggcag aagugcggag uuacugcuau      780

gaggcuagca uaucugauau ggccuccgac ucacguuguc ccacacaagg agaagcguac      840

cuugacaaac agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg      900

gguaaugggu guggacuguu cggcaaaggc agucuaguca caugcgccaa guuugcaugc      960

ucuaagaaaa ugaccgggaa guccauucag ccugagaacc ucgaauacag gaucaugcug     1020

agcgugcacg gaucgcaaca cucugguaug auuguuaaug auacgggcca ugagaccgac     1080

gagaaccggg cuaaagugga aaucacacca aauucacccc gagccgaggc uacucuuggg     1140

ggauucggcu cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug     1200

uauuacuuaa caaugaacaa uaagcacugg cugguccaua aagagugguu ccacgacauc     1260

ccacucccgu ggcacgccgg cgcagauacu ggaacacccc auuggaacaa uaaggaagcc     1320

cuuguggagu uuaaagacgc ucacgcgaag agacagaccg uaguugugcu gggcagccag     1380

gagggggcag uccauaccgc cuuggcugga gcccuagaag cagagaugga uggugccaag     1440

ggcaggcugu ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag     1500

ggagugagcu auucauugug uacugcugcc uucacguuua caaaaauucc ugcagaaacc     1560

cuccauggca cugucaccgu ggagguucag uacgcgggua cagacgggcc augcaaggug     1620

cccgcucaaa uggccguaga uaugcagacc cugacuccug ucggcagauu aauaacagca     1680

aacccaguga ucacggaaag uaccgagaac uccaagauga ugcuggagcu agaucccccg     1740

uucggagaca gcuacauugu uaucggcgug ggggagaaaa agauaacuca ccacuggcau     1800

aggucuggau caacaauugg uaaagccuuu gaagcuaccg uccguggcgc caagcggaug     1860

gcagugcucg gggacacagc uugggauuuc ggaaguguag gcggugcccu gaauagccuu     1920

gggaaaggca uccaccagau cuucggagcg gccuuuaagu ccuuguucgg cgggaugucu     1980

ugguuuucgc aaauucugau cggaacucuc cugauguggc uuggccugaa caccaagaau     2040

gguagcauuu ccuugaugug ucucgcuuua gggggagugc ugauauuccu gucaacggca     2100

guuagugccg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8330
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8330
gggagaaagc uuaccaugaa ggagaaaaag agaaggggcg ccgacaccag cguggggauc       60

gucggacugc uccuuacaac ugcuauggca gccgaaguua cgcggcgcgg uuccgcguac      120

uauauguacc uggaucgaaa cgacgcuggc gaggccauuu cuuuccccac cacauugggg      180

augaauaaau gcuauauaca gaucauggau uuaggacaca ugugugacgc aacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcccguaga      360

agcaggcggg cugugacacu accgucccau ucuacccgca agcuccaaac gagaucgcag      420

acuuggcugg agucaaggga auacaccaag caccuuauuc ggguugagaa uuggaucuuu      480

cgcaaccccg gauucgcacu ggccgcugcc gcgauugcau gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugaua cugcucaucg ccccugcuua cucuauucga      600

ugcaucggcg ugagcaauag agauuuugua gaagggaugu ccggagguac uuggguugac      660

auuguguuag agcauggcgg gugugucacc gugauggccc aggauaagcc aacaguggac      720

aucgagcugg ucaccacgac uguuucaaac auggcagaag ugaggaguua cugcuaugag      780

gcuagcauau cugauauggc cuccgacuca cgguguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guauguaugc aagcguacuu ugguggaccg cggcuggggu      900

aaugggugug gacuguucgg caaaggcagu cuagucacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaccucg aauacagaau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauu guuaaugaua cgggccauga gaccgacgag     1080

aacagggcua aaguggaaau cacaccaaau ucaccccggg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagcgc cagaccguag uugugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

agacuguccu cugggcaccu caaaugcagg cugaagaugg acaaacuucg gcugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauuccugc agaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gccgcuuaau aacagcaaac     1680

ccagugauca cggaaaguac cgagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauuguuau cggcgugggg gagaaaaaga uaacucacca cuggcauaga     1800

ucuggaucaa caauugguaa agccuuugaa gcuaccguca ggggcgccaa gcguauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

agcauuuccu ugaugugucu cgcuuuaggg ggagugcuga uauuccuguc aacggcaguu     2100

agugccgacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8331
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8331
gggagaaagc uuaccaugga gaagaaaaga aggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc uuacaacugc uauggcagcc gaaguuacgc ggcgcgguuc cgcguacuau      120

auguaccugg aucgaaacga cgcuggcgag gccauuucuu uccccaccac auuggggaug      180

aauaagugcu auauacagau cauggauuua ggacacaugu gugacgcaac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaaa agggggaggc ccguagaagc      360

aggcgggcug ugacacuacc gucccauucu acccgcaaac uccaaacgag aucgcagacu      420

uggcuggagu caagggaaua caccaagcac cuuauucggg uugagaauug gaucuuucgc      480

aaccccggau ucgcacuggc cgcugccgcg auugcauggu ugcucggcag caguacaucc      540

cagaagguga ucuaucuggu caugauacug cucaucgccc cugcuuacuc uauucgaugc      600

aucggcguga gcaauagaga uuuuguagaa gggauguccg gagguacuug gguugacauu      660

guguuagagc auggcgggug ugucaccgug auggcccagg auaaaccaac aguggacauc      720

gagcugguca ccacgacugu uucaaacaug gcagaaguga ggaguuacug cuaugaggcu      780

agcauaucug auauggccuc cgacucacgg ugucccacac aaggagaagc guaccuugac      840

aagcagagcg auacccagua uguaugcaaa cguacuuugg uggaccgcgg cugggguaau      900

ggguguggac uguucggcaa gggcagucua gucacaugcg ccaaauuugc augcucuaag      960

aagaugaccg ggaaauccau ucagccugag aaccucgaau acagaaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugauuguu aaugauacgg gccaugagac cgacgagaac     1080

agggcuaagg uggaaaucac accaaauuca ccccgggccg aggcuacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucga accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga acaauaaaca cuggcugguc cauaaggagu gguuccacga caucccacuc     1260

ccguggcacg ccggcgcaga uacuggaaca ccccauugga acaauaaaga agcccuugug     1320

gaguuuaagg acgcucacgc gaaacgccag accguaguug ugcugggcag ccaggagggg     1380

gcaguccaua ccgccuuggc uggagcccua gaagcagaga uggauggugc caagggcaga     1440

cuguccucug ggcaccucaa gugcaggcug aaaauggaca agcugcggcu gaaaggagug     1500

agcuauucau uguguacugc ugccuucacg uuuacaaaga uuccugcaga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcg gguacagacg ggccaugcaa agugcccgcu     1620

caaauggccg uagauaugca gacccugacu ccugucggcc gcuuaauaac agcaaaccca     1680

gugaucacgg aaaguaccga gaacuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uuguuaucgg cgugggggaa aagaaaauaa cucaccacug gcauagaucu     1800

ggaucaacaa uugguaaggc cuuugaggcu accgucaggg gcgccaaacg uauggcagug     1860

cucggggaca cagcuuggga uuucggaagu guaggcggug cccugaauag ccuugggaag     1920

ggcauccacc agaucuucgg agcggccuuu aaauccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugaucggaac ucuccugaug uggcuuggcc ugaacaccaa gaaugguagc     2040

auuuccuuga ugugucucgc ucuuggggga guguuaauau uccugucaac ggcaguuagu     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8332
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8332
gggagaaagc uuaccaugaa gaaaagaagg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuua caacugcuau ggcagccgag guuacgcggc gcgguuccgc guacuauaug      120

uaccuggauc gaaacgacgc uggcgaagcc auuucuuucc ccaccacauu ggggaugaau      180

aagugcuaua uacagaucau ggauuuagga cacaugugug acgcaacuau gucauacgag      240

ugcccuaugc uggaugaagg cguggagcca gacgauguag acuguuggug caacaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaaaaagg gggaagcccg uagaagcagg      360

cgggcuguga cacuaccguc ccauucuacc cgcaaacucc aaacgagauc gcagacuugg      420

cuggagucaa gggaguacac caagcaccuu auucggguug aaaauuggau cuuucgcaac      480

cccggauucg cacuggccgc ugccgcgauu gcaugguugc ucggcagcag uacaucccag      540

aaggugaucu aucuggucau gauacugcuc aucgccccug cuuacucuau ucgaugcauc      600

ggcgugagca auagagauuu uguagagggg auguccggag guacuugggu ugacauugug      660

uuagaacaug gcgggugugu caccgugaug gcccaggaua aaccaacagu ggacaucgag      720

cuggucacca cgacuguuuc aaacauggca gaggugagga guuacugcua ugaagcuagc      780

auaucugaua uggccuccga cucacggugu cccacacaag gagaggcgua ccuugacaag      840

cagagcgaua cccaguaugu augcaaacgu acuuuggugg accgcggcug ggguaauggg      900

uguggacugu ucggcaaggg cagucuaguc acaugcgcca aauuugcaug cucuaagaag      960

augaccggga aauccauuca gccugaaaac cucgaguaca gaaucaugcu gagcgugcac     1020

ggaucgcaac acucugguau gauuguuaau gauacgggcc augaaaccga cgagaacagg     1080

gcuaaggugg agaucacacc aaauucaccc cgggccgaag cuacucuugg gggauucggc     1140

ucccuggguu uggauuguga gccucgaacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgacau cccacucccg     1260

uggcacgccg gcgcagauac uggaacaccc cauuggaaca auaaagaggc ccuuguggaa     1320

uuuaaggacg cucacgcgaa acgccagacc guaguugugc ugggcagcca ggagggggca     1380

guccauaccg ccuuggcugg agcccuagag gcagaaaugg auggugccaa gggcagacug     1440

uccucugggc accucaagug caggcugaaa auggacaagc ugcggcugaa aggagugagc     1500

uauucauugu guacugcugc cuucacguuu acaaagauuc cugcagagac ccuccauggc     1560

acugucaccg uggaaguuca guacgcgggu acagacgggc caugcaaagu gcccgcucaa     1620

auggccguag auaugcagac ccugacuccu gucggccgcu uaauaacagc aaacccagug     1680

aucacggaga guaccgaaaa cuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacauug uuaucggcgu gggggagaag aaaauaacuc accacuggca uagaucugga     1800

ucaacaauug guaaggccuu ugaagcuacc gucaggggcg ccaaacguau ggcagugcuc     1860

ggggacacag cuugggauuu cggaagugua ggcggugccc ugaauagccu ugggaagggc     1920

auccaccaga ucuucggagc ggccuuuaaa uccuuguucg gcgggauguc uugguuuucg     1980

caaauucuga ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu     2040

uccuugaugu gucucgcucu ugggggagug uuaauauucc ugucaacggc aguuagugcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8333
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8333
gggagaaagc uuaccaugaa gagaaggggc gccgacacca gcguggggau cgucggacug       60

cuccuuacaa cugcuauggc agccgagguu acgcggcgcg guuccgcgua cuauauguac      120

cuggaucgaa acgacgcugg cgaagccauu ucuuucccca ccacauuggg gaugaauaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg caacuauguc auacgagugc      240

ccuaugcugg augaaggcgu ggagccagac gauguagacu guuggugcaa caccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aagcccguag aagcaggcgg      360

gcugugacac uaccguccca uucuacccgc aagcuccaaa cgagaucgca gacuuggcug      420

gagucaaggg aguacaccaa acaccuuauu cggguugaaa auuggaucuu ucgcaacccc      480

ggauucgcac uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaag      540

gugaucuauc uggucaugau acugcucauc gccccugcuu acucuauucg augcaucggc      600

gugagcaaua gagauuuugu agaggggaug uccggaggua cuuggguuga cauuguguua      660

gaacauggcg ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa cauggcagag gugaggaguu acugcuauga agcuagcaua      780

ucugauaugg ccuccgacuc acgguguccc acacaaggag aggcguaccu ugacaaacag      840

agcgauaccc aguauguaug caagcguacu uugguggacc gcggcugggg uaaugggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaaaaagaug      960

accgggaagu ccauucagcc ugaaaaccuc gaguacagaa ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaaugau acgggccaug aaaccgacga gaacagggcu     1080

aaaguggaga ucacaccaaa uucaccccgg gccgaagcua cucuuggggg auucggcucc     1140

cuggguuugg auugugagcc ucgaaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaacaaua agcacuggcu gguccauaaa gaaugguucc acgacauccc acucccgugg     1260

cacgccggcg cagauacugg aacaccccau uggaacaaua aggaggcccu uguggaauuu     1320

aaagacgcuc acgcgaagcg ccagaccgua guugugcugg gcagccagga gggggcaguc     1380

cauaccgccu uggcuggagc ccuagaggca gaaauggaug gugccaaagg cagacugucc     1440

ucugggcacc ucaagugcag gcugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucauugugua cugcugccuu cacguuuaca aaaauuccug cagagacccu ccauggcacu     1560

gucaccgugg aaguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug     1620

gccguagaua ugcagacccu gacuccuguc ggccgcuuaa uaacagcaaa cccagugauc     1680

acggagagua ccgaaaacuc caaaaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaagaag auaacucacc acuggcauag aucuggauca     1800

acaauuggua aagccuuuga agcuaccguc aggggcgcca agcguauggc agugcucggg     1860

gacacagcuu gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc     1920

caccagaucu ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaca ccaaaaaugg uagcauuucc     2040

uugauguguc ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccgac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8334
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8334
gggagaaagc uuaccaugag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug cuauggcagc cgagguuacg cggcgcgguu ccgcguacua uauguaccug      120

gaucgaaacg acgcuggcga agccauuucu uuccccacca cauuggggau gaauaagugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgagugcccu      240

augcuggaug aaggcgugga gccagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaaa aagggggaag cccguagaag caggcgggcu      360

gugacacuac cgucccauuc uacccgcaaa cuccaaacga gaucgcagac uuggcuggag      420

ucaagggagu acaccaagca ccuuauucgg guugaaaauu ggaucuuucg caaccccgga      480

uucgcacugg ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug      600

agcaauagag auuuuguaga ggggaugucc ggagguacuu ggguugacau uguguuagaa      660

cauggcgggu gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcagaggug aggaguuacu gcuaugaagc uagcauaucu      780

gauauggccu ccgacucacg gugucccaca caaggagagg cguaccuuga caagcagagc      840

gauacccagu auguaugcaa acguacuuug guggaccgcg gcugggguaa ugggugugga      900

cuguucggca agggcagucu agucacaugc gccaaauuug caugcucuaa gaaaaugacc      960

gggaagucca uucagccuga aaaccucgag uacagaauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaaa ccgacgagaa cagggcuaag     1080

guggagauca caccaaauuc accccgggcc gaagcuacuc uugggggauu cggcucccug     1140

gguuuggauu gugagccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu ccauaaggaa ugguuccacg acaucccacu cccguggcac     1260

gccggcgcag auacuggaac accccauugg aacaauaaag aggcccuugu ggaauuuaag     1320

gacgcucacg cgaaacgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau     1380

accgccuugg cuggagcccu agaggcagaa auggauggug ccaagggcag acuguccucu     1440

gggcaccuca aaugcaggcu gaagauggac aagcugcggc ugaaaggagu gagcuauuca     1500

uuguguacug cugccuucac guuuacaaag auuccugcag agacccucca uggcacuguc     1560

accguggaag uucaguacgc ggguacagac gggccaugca aagugcccgc ucaaauggcc     1620

guagauaugc agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca     1800

auugguaagg ccuuugaagc uaccgucagg ggcgccaaac guauggcagu gcucggggac     1860

acagcuuggg auuucggaag uguaggcggu gcccugaaua gccuugggaa gggcauccac     1920

cagaucuucg gagcggccuu uaaauccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cucuuggggg aguguuaaua uuccugucaa cggcaguuag ugccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8335
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8335
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga gguuacgagg cgggguuccg cguacuauau guaccuggau      120

cgcaacgacg cuggcgaagc cauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga gugcccuaug      240

cuggaugaag gcguggagcc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaagccc gacguagcag aagggcugug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaguca      420

agagaguaca ccaagcaccu uauuaggguu gaaaauugga ucuuucggaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaggg gauguccgga gguacuuggg uugacauugu guuagaacau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaggugaga aguuacugcu augaagcuag cauaucugau      780

auggccuccg acucaaggug ucccacacaa ggagaggcgu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugaaaa ccucgaguac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugaaaccg acgagaacag agcuaaggug     1080

gagaucacac caaauucccc cagggccgaa gcuacucuug ggggauucgg cucacugggu     1140

uuggauugug agccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggaaugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaagagg cccuugugga auuuaaggac     1320

gcucacgcga aacgacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga ggcagaaaug gauggugcca agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaagauu ccugcagaga cccuccaugg cacugucacc     1560

guggaaguuc aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag caaacccagu gaucacggag     1680

aguaccgaaa acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggccu uugaagcuac cgucagaggc gccaaaagga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuc uugggggagu guuaauauuc cugucaacgg caguuagugc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8336
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8336
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaggu uacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

aacgacgcug gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug      240

gaugaaggcg uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caaaaagggg gaagcccgcc gaagccguag agcugugaca      360

cuaccguccc auucuaccag gaaacuccaa acgcggucgc agacuuggcu ggagucacgc      420

gaguacacca agcaccuuau uagaguugaa aauuggaucu uuaggaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc ggugcaucgg cgugagcaau      600

cgcgauuuug uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga ggugcgaagu uacugcuaug aagcuagcau aucugauaug      780

gccuccgacu caagaugucc cacacaagga gaggcguacc uugacaagca gagcgauacc      840

caguauguau gcaaaaggac uuugguggac cggggcuggg guaaugggug uggacuguuc      900

ggcaagggca gucuagucac augcgccaaa uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugaaaaccu cgaguaccgu aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gaaaccgacg agaaccgcgc uaagguggag     1080

aucacaccaa auucccccag agccgaagcu acucuugggg gauucggcuc acuggguuug     1140

gauugugagc cuaggaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aaacacuggc ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu     1320

cacgcgaaac ggcagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaggc agaaauggau ggugccaagg gccgacuguc cucugggcac     1440

cucaaaugcc gccugaagau ggacaagcug agacugaaag gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug     1560

gaaguucagu acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggcagguua auaacagcaa acccagugau cacggagagu     1680

accgaaaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcauc ggucuggauc aacaauuggu     1800

aaggccuuug aagcuaccgu ccgcggcgcc aaaagaaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc     1920

uucggagcgg ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcucuug ggggaguguu aauauuccug ucaacggcag uuagugccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8337
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8337
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccuuacaacu       60

gcuauggcag ccgagguuac gagaagggga uccgcguacu auauguaccu ggaucggaac      120

gacgcuggug aagccauuuc uuuccccacc acauugggca ugaauaagug cuauauacag      180

aucauggauu uagggcacau gugugacgca acuaugucau acgagugccc uaugcuggau      240

gaaggagugg agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uauggcacuu gucaucacaa aaagggugaa gcccgccgaa gccguagagc ugugacacua      360

ccgucccauu cuaccaggaa acuccaaacg cggucgcaga cuuggcugga gucacgcgag      420

uacaccaagc accuuauuag aguugaaaau uggaucuuua ggaaccccgg guucgcacug      480

gccgcugccg cgauugcaug guugcucgga agcaguacau cccagaaagu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucggu gcaucggcgu gagcaaucgc      600

gauuuuguag agggcauguc cgggggaacu uggguugaca uuguguuaga acaugguggc      660

ugugucaccg ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaggu gcgaaguuac ugcuaugaag cuagcauauc ugauauggcc      780

uccgacucaa gaugucccac acaaggggag gcguaccuug acaagcagag cgauacccag      840

uauguaugca aaaggacuuu gguggaccgg ggauggggca augguugugg gcuguucgga      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaaaaugac cggcaagucc      960

auucagccug aaaaccucga guaccguauc augcugagcg ugcacggguc gcaacacucu     1020

ggaaugauug uuaaugauac gggucaugaa accgacgaga accgcgcuaa gguggagauc     1080

acaccaaauu cccccagagc cgaagcuacu cuuggcgggu ucggaucacu gggcuuggau     1140

ugugagccua ggaccggucu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gacaucccac ucccguggca cgccggggca     1260

gauacuggca caccccauug gaacaauaaa gaggcccuug uggaauuuaa ggacgcucac     1320

gcgaaacggc agaccguagu ugugcuggga agccaggagg gcgcagucca uaccgccuug     1380

gcuggggccc uagaggcaga aauggaugga gccaaggguc gacuguccuc uggccaccuc     1440

aaaugccgcc ugaagaugga caaacuuaga cugaaggggg ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gagacccucc auggaacugu caccguggaa     1560

guucaguacg cgggcacaga cgguccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg gagguuaaua acagcaaacc cagugaucac ggagaguacc     1680

gaaaacucca agaugaugcu ggagcuagau cccccguucg gcgacagcua cauuguuauc     1740

ggagugggcg agaaaaagau aacucaccac uggcaucggu cugggucaac aauuggaaag     1800

gccuuugaag cuaccguccg cggugccaaa agaauggcag ugcucggcga cacagcuugg     1860

gauuucggga guguaggagg cgcccugaau agccuuggua aggggaucca ccagaucuuc     1920

ggcgcggccu uuaaauccuu guucggaggc augucuuggu uuucgcaaau ucugaucggg     1980

acucuccuga uguggcuugg acugaacacc aagaauggca gcauuuccuu gaugugucuc     2040

gcucugggug ggguguuaau auuccuguca acggcaguua gugccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8338
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8338
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu uacaacugcc       60

auggcugcag agguuacgag aaggggaucc gccuacuaua uguaccugga ucggaacgac      120

gcgggugaag cuauuucuuu ccccaccaca uugggcauga auaagugcua uauacagauc      180

auggauuuag ggcacaugug ugacgccacu augucauacg agugcccuau gcuggaugaa      240

ggaguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

ggcacuuguc aucacaaaaa gggugaagca cgccgaagcc guagagccgu gacacuaccg      360

ucccauucua ccaggaaacu ccaaacgcgg ucgcagacuu ggcuggaguc acgcgaguac      420

accaagcacc uuauuagagu ugaaaauugg aucuuuagga accccggguu cgcucuggca      480

gccgcugcca uugcgugguu gcucggaagc aguacauccc agaaagugau cuaucugguc      540

augauacugc ucaucgcacc cgccuacucu auucggugca ucggcgugag caaucgcgau      600

uuuguagagg gcauguccgg gggaacuugg guugacauug uguuagaaca ugguggcugu      660

gucaccguga uggcucagga uaagccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg ccgaggugcg aaguuacugc uaugaagcaa gcauaucuga uauggcuucc      780

gacucaagau gucccacaca aggggaggcc uaccuugaca agcagagcga uacccaguau      840

guaugcaaaa ggacuuuggu ggaccgggga uggggcaaug guugugggcu guucggaaag      900

ggcagucuag ucacaugcgc gaaauuugcc ugcucuaaga aaaugaccgg caaguccauu      960

cagccugaaa accucgagua ccguaucaug cugagcgugc acgggucgca acacucugga     1020

augauuguua augauacggg ucaugaaacc gacgagaacc gcgcaaaggu ggagaucaca     1080

ccaaauuccc ccagagcuga agccacucuu ggcggguucg gaucacuggg cuuggauugu     1140

gagccuagga ccggucucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc uggggccgau     1260

acuggcaccc cucauuggaa caauaaagag gcacuugugg aauuuaagga cgcccacgcu     1320

aaacggcaga caguaguugu gcugggaagc caggagggcg cgguccauac cgcauuggcc     1380

ggggcucuag aggccgaaau ggauggagca aagggucgac uguccucugg ccaccucaaa     1440

ugccgccuga agauggacaa acuuagacug aaggggguga gcuauucauu guguacugcc     1500

gcuuucacgu uuacaaagau uccugccgag acccuccaug gaacugucac cguggaaguu     1560

caguacgcag gcacagacgg uccaugcaaa gugcccgcgc aaauggcugu agauaugcag     1620

acccugacuc cugucgggag guuaauaaca gccaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggcg acagcuacau uguuaucgga     1740

gugggcgaga aaaagauaac ucaccacugg caucggucug ggucaacaau uggaaaggca     1800

uuugaagcca ccguccgcgg ugcuaaaaga auggccgugc ucggcgacac agcaugggau     1860

uucgggagug uaggaggcgc ucugaauagc cuugguaagg ggauccacca gaucuucggc     1920

gccgcguuua aauccuuguu cggaggcaug ucuugguuuu cgcaaauucu gaucgggacu     1980

cuccugaugu ggcuuggacu gaacaccaag aauggcagca uuuccuugau gugucucgcc     2040

cugggugggg uguuaauauu ccugucaacg gcuguuagug cagacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  8339
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8339
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcag auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8340
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8340
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagccg uuacgagaag gggauccgcg uacuauaugu accuggaccg gaacgaugcu      120

ggugaggcca uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcaacuaug ucauacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaggcccgc cgaagccgua gagcugugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggaaucacg cgaguacacc      420

aagcaccuua uuagaguuga gaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagaaggca uguccggggg aacuuggguu gauauugugu uagagcaugg uggcuguguc      660

accgugaugg cccaggacaa gccaacagug gauaucgaac uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaggcuagca uaucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaagcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugagaacc ucgaauaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugagaccgau gaaaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaggc uacucuuggc ggguucggau cacugggcuu ggacugugaa     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggagugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacacu     1260

ggcacacccc auuggaacaa uaaagaagcc cuuguggagu uuaaggaugc ucacgcgaaa     1320

cggcagaccg uaguugugcu gggaagccag gaaggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagagaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagaaacc cuccauggaa cugucaccgu ggagguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaggcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccg auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8341
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8341
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccgc gguuacgagg cgggguuccg cuuacuauau guaccuggau      120

cgcaacgacg ccggcgaggc aauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgccacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaggcuc gacguagcag aagggcagug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaauca      420

agagaguaca ccaagcaccu uauuaggguu gagaauugga ucuuucggaa ccccggauuc      480

gcccuggcug ccgcggcaau ugccugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgcuccu gccuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcacaggau aagccaacag uggacaucga acuggucacc      720

acgacuguuu caaacauggc ugaggugaga aguuacugcu augaggccag cauaucugau      780

auggcguccg acucaaggug ucccacacaa ggagaagccu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgca aaauuugcuu gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgaaaacag agccaaggug     1080

gagaucacac caaauucccc cagggcugag gccacucuug ggggauucgg cucacugggu     1140

uuggauugug aaccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgca     1260

ggcgccgaua cuggaacacc ccauuggaac aauaaagaag cucuugugga guuuaaggac     1320

gcgcacgcaa aacgacagac cguaguugug cugggcagcc aggaaggggc cguccauacc     1380

gcuuuggccg gagcacuaga ggccgagaug gauggugcua agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugccg cauucacguu uacaaagauu ccugcggaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcugg uacagacggg ccaugcaaag ugcccgccca aauggcagua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag ccaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggaa cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggcuu uugaggccac cgucagaggc gcaaaaagga uggcugugcu cggggacaca     1860

gccugggauu ucggaagugu aggcggugcg cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag ccgcuuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcac uugggggagu guuaauauuc cugucaacgg ccguuagugc ugacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8342
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8342
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagguguuac ucgacguggc      120

ucagccuacu auauguaccu ggauagaaac gacgcugggg aggcaauuag uuuucccacg      180

acccuaggaa ugaauaaaug cuauauacaa aucauggauc ucggccauau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggugugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugggaccu gucaccauaa gaaaggagag      360

gcgaggcggu cucgcagagc ugugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg cuuugcccug gcagccgcug caauugccug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc uccugccuac      600

uccauuaggu gcaucggggu gucaaaucgg gauuucguag aaggaaugag ugguggcaca      660

uggguugaca uuguguugga gcacggggga ugugucacug ugauggcgca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggcagaggu gcguucuuac      780

ugcuaugagg ccuccauauc agauauggcu agcgacaguc gcugucccac ucagggcgaa      840

gccuaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

gguuggggga auggaugugg ccuguuuggc aaaggguccc uugucacuug cgcaaaguuc      960

gcuugcagca aaaagaugac aggaaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacgguuc ccagcauuca ggcaugauug uuaaugauac cgggcacgag     1080

acggacgaaa accgagccaa aguggagauc accccaaaua guccccgcgc ggaggccaca     1140

cucggaggcu uugguagccu gggguuagau ugugaaccua gaacuggccu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcaggagcu gauacaggca cuccccacug gaacaauaag     1320

gaagcccuag uggaguucaa agacgcucau gccaagaggc agaccguagu ugugcugggg     1380

ucucaggaag gagcagucca cacagcccuc gcuggugcgc uggaggcaga gauggauggc     1440

gccaaagggc ggcuuagcuc aggacaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggcg ugaguuauuc cuuauguacc gcugccuuua cuuucacgaa aauuccugca     1560

gaaacacugc acgguaccgu cacuguggag guucaauacg ccgggaccga cggcccaugc     1620

aaggugcccg cucagauggc cguagauaug cagacacuaa ccccugucgg aaggcucaua     1680

acugcaaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gcgacucaua cauuguuauc ggggugggag agaagaagau aacccaccau     1800

uggcaccgua gugguagcac uauuggcaaa gcguucgagg cuacaguccg aggggccaag     1860

cggauggcag uguugggaga caccgccugg gauuucggcu ccguaggugg ggcucucaau     1920

ucucugggca aaggaaucca ucaaaucuuu ggcgccgcau ucaagucgcu guuuggggga     1980

augagcuggu ucucccagau ucuuaucggc acacuguuga uguggcucgg ucugaacacu     2040

aaaaaugggu caauuaguuu aaugugucug gcucuaggag gcgugcucau auuucugucu     2100

accgccguua gcgcggacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8343
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8343
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagccguuac ucgacguggu      120

ucagcuuacu auauguaccu ggauagaaac gacgcaggcg aggccauuag uuuucccacg      180

acccuaggga ugaauaaaug cuauauacaa aucauggauc ucggacauau gugugacgcg      240

acaaugagcu acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugguaccu gucaccauaa gaaaggggag      360

gcuaggcggu cucgcagagc cgugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg auuugcacug gccgcugcag ccauugcuug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc cccugcguac      600

uccauuaggu gcaucggcgu gucaaaucgg gauuucguag aagggaugag uggagguaca      660

uggguugaca uuguguugga gcacggcggg ugugucacug ugauggcaca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggccgaggu gcguucuuac      780

ugcuaugagg cuuccauauc agauauggcc agcgacaguc gcugucccac ucagggagaa      840

gcauaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

ggcuggggua augggugugg acuguuuggc aaaggcuccc uugucacuug cgcuaaguuc      960

gccugcagca aaaagaugac agggaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacggauc ccagcauuca gguaugauug uuaaugauac cggccacgag     1080

acggacgaaa accgagcgaa aguggagauc accccaaaua guccccgcgc cgaggcaaca     1140

cucgggggau uuggcagccu ggguuuagau ugugaaccua gaacugggcu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcuggcgcc gauacaggaa cuccccacug gaacaauaag     1320

gaagcucuag uggaguucaa agacgcccau gcaaagaggc agaccguagu ugugcugggc     1380

ucucaggaag gggccgucca cacagcucuc gcgggagcac uggaggccga gauggauggu     1440

gcuaaaggcc ggcuuagcuc agggcaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggag ugaguuauuc cuuauguacc gccgcauuua cuuucacgaa aauuccugcc     1560

gaaacacugc acggcaccgu cacuguggag guucaauacg cugguaccga cgggccaugc     1620

aaggugcccg cccagauggc aguagauaug cagacacuaa ccccugucgg caggcucaua     1680

acugcgaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gagacucaua cauuguuauc ggcguggggg agaagaagau aacccaccau     1800

uggcaccgua guggaagcac uauugguaag gcuuucgagg ccacaguccg gggcgcaaaa     1860

cgaauggccg uguuggggga caccgcuugg gauuucggau ccguaggcgg ugcccucaau     1920

ucucugggga aaggcaucca ucaaaucuuu ggagcagcuu ucaagucgcu guuuggcggg     1980

augagcuggu ucucccagau ucuuaucgga acacuguuga uguggcucgg ccugaacacu     2040

aaaaaugguu caauuaguuu aaugugucug gcccuagggg gagugcucau auuucugucu     2100

accgcgguua gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8344
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8344
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcuugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8345
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8345
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agaggcgggg cgcugacacc       60

agcgugggga uugucggacu gcuccuuaca acugcaaugg ccgcggaagu uacgcgccga      120

gguuccgcuu acuauaugua ccuggaucgu aaugacgccg gcgaggcaau aucuuucccc      180

accacauugg ggaugaacaa augcuauauc cagauuaugg auuuaggaca caugugugac      240

gccacuaugu cauacgaaug cccuaugcug gaugagggcg uggaaccaga cgauguagac      300

uguuggugca auaccacaag uaccugggug gucuauggua cuugucauca caagaaaggg      360

gaggcuagaa ggagccggcg cgcagugaca cuaccguccc auucuaccag aaagcuccaa      420

acgaggucgc agacuuggcu ggagucacgg gaauacacca agcaccuuau ccgcguugag      480

aacuggauuu uucgaaaucc cggauucgcc cuggcugccg cggcaaucgc cugguugcuc      540

ggcagcagua caucccagaa agugauauau cuggucauga uccugcucau ugcuccugcc      600

uacucuauca gaugcauugg cgugagcaac agggauuuug uagaagggau guccggaggu      660

acuuggguug acaucguguu agagcauggc ggguguguca ccgugauggc acaggauaag      720

ccaacagugg acauagagcu ggucaccacg acuguuucaa auauggcuga agugcggagu      780

uacugcuaug aggccagcau uucugauaug gcguccgacu cacguugucc cacacaagga      840

gaagccuacc uugacaaaca gagcgauacc caguauguau gcaagcgcac uuugguggac      900

agaggcuggg guaacgggug uggacuguuc ggcaaaggca gucuagucac augcgcaaag      960

uuugcuugcu cuaagaaaau gaccgggaag uccauccagc cugagaaucu cgaauacagg     1020

auuaugcuga gcgugcacgg aucgcaacac ucugguauga ucguuaacga uacgggccau     1080

gagaccgacg agaaucgggc caaaguggaa aucacaccaa acucaccccg agcugaggcc     1140

acucuugggg gauucggcuc ccuggguuug gauugugaac cucgcaccgg gcucgacuuu     1200

agugaucugu auuacuuaac aaugaauaac aagcacuggc ugguccauaa agagugguuc     1260

cacgacauuc cacucccgug gcacgcaggc gccgauacug gaacacccca uuggaauaac     1320

aaggaagcuc uuguggaguu uaaagacgcg cacgcaaaga gacagaccgu aguugugcug     1380

ggcagccagg agggggccgu ccauaccgcu uuggccggag cacuagaagc cgagauggau     1440

ggugcuaagg gcaggcuguc cucugggcac cucaaaugcc ggcugaagau ggacaaacuu     1500

cgccugaagg gagugagcua uucauugugu acugccgcau ucacguuuac aaaaauaccu     1560

gcggaaaccc uccauggcac ugucaccgug gagguucagu acgcugguac agacgggcca     1620

ugcaaggugc ccgcccaaau ggcaguagau augcagaccc ugacuccugu cggcagauua     1680

aucacagcca acccagugau uacggaaagu accgaaaauu ccaagaugau gcuggagcua     1740

gaucccccgu ucggagacag cuacaucguu auaggcgugg gggagaaaaa gauuacucac     1800

cacuggcaua ggucuggauc aacaaucggu aaggcuuuug aggccaccgu ccguggcgca     1860

aaacggaugg cugugcucgg ggacacagcc ugggauuucg gaaguguagg cggugcgcug     1920

aacagccuug ggaaaggcau ccaccagauu uucggagccg cuuuuaaguc cuuguucggc     1980

gggaugucuu gguuuucgca aauccugauu ggaacucucc ugauguggcu uggccugaau     2040

accaagaacg guagcauauc cuugaugugu cucgcauuag ggggagugcu gaucuuccug     2100

ucaacggccg uuagugcuug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8346
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8346
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagaggcg gggcgcugac       60

accagcgugg ggauagucgg acugcuccuu acaacugcaa uggccgcgga aguuacgcgc      120

cgagguuccg cuuacuauau guaccuggau cguaaugacg ccggcgaggc aaucucuuuc      180

cccaccacau uggggaugaa caaaugcuau auucagauca uggauuuagg acacaugugu      240

gacgccacua ugucauacga augcccuaug cuggaugagg gcguggaacc agacgaugua      300

gacuguuggu gcaauaccac aaguaccugg guggucuaug guacuuguca ucacaagaaa      360

ggggaggcua gaaggagccg gcgcgcagug acacuaccgu cccauucuac cagaaagcuc      420

caaacgaggu cgcagacuug gcuggaguca cgggaauaca ccaagcaccu uauucgcguu      480

gagaacugga ucuuucgaaa ucccggauuc gcccuggcug ccgcggcaau agccugguug      540

cucggcagca guacauccca gaaagugauc uaucugguca ugauucugcu caucgcuccu      600

gccuacucua uuagaugcau cggcgugagc aacagggauu uuguagaagg gauguccgga      660

gguacuuggg uugacauagu guuagagcau ggcgggugug ucaccgugau ggcacaggau      720

aagccaacag uggacauuga gcuggucacc acgacuguuu caaauauggc ugaagugcgg      780

aguuacugcu augaggccag caucucugau auggcguccg acucacguug ucccacacaa      840

ggagaagccu accuugacaa acagagcgau acccaguaug uaugcaagcg cacuuuggug      900

gacagaggcu gggguaacgg guguggacug uucggcaaag gcagucuagu cacaugcgca      960

aaguuugcuu gcucuaagaa aaugaccggg aaguccauuc agccugagaa ucucgaauac     1020

aggaucaugc ugagcgugca cggaucgcaa cacucuggua ugaucguuaa cgauacgggc     1080

caugagaccg acgagaaucg ggccaaagug gaaauuacac caaacucacc ccgagcugag     1140

gccacucuug ggggauucgg cucccugggu uuggauugug aaccucgcac cgggcucgac     1200

uuuagugauc uguauuacuu aacaaugaau aacaagcacu ggcuggucca uaaagagugg     1260

uuccacgaca uaccacuccc guggcacgca ggcgccgaua cuggaacacc ccauuggaau     1320

aacaaggaag cucuugugga guuuaaagac gcgcacgcaa agagacagac cguaguugug     1380

cugggcagcc aggagggggc cguccauacc gcuuuggccg gagcacuaga agccgagaug     1440

gauggugcua agggcaggcu guccucuggg caccucaaau gccggcugaa gauggacaaa     1500

cuucgccuga agggagugag cuauucauug uguacugccg cauucacguu uacaaaaauc     1560

ccugcggaaa cccuccaugg cacugucacc guggagguuc aguacgcugg uacagacggg     1620

ccaugcaagg ugcccgccca aauggcagua gauaugcaga cccugacucc ugucggcaga     1680

uuaauuacag ccaacccagu gaucacggaa aguaccgaaa auuccaagau gaugcuggag     1740

cuagaucccc cguucggaga cagcuacaua guuauuggcg ugggggagaa aaagaucacu     1800

caccacuggc auaggucugg aucaacaauc gguaaggcuu uugaggccac cguccguggc     1860

gcaaaacgga uggcugugcu cggggacaca gccugggauu ucggaagugu aggcggugcg     1920

cugaacagcc uugggaaagg cauucaccag aucuucggag ccgcuuuuaa guccuuguuc     1980

ggcgggaugu cuugguuuuc gcaaauucug auaggaacuc uccugaugug gcuuggccug     2040

aauaccaaga acgguagcau cuccuugaug ugucucgcau uagggggagu gcugauuuuc     2100

cugucaacgg ccguuagugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8347
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8347
gggagaaagc uuaccaugaa cgccagaaag gagaaaaaga ggcggggcgc ugacaccagc       60

guggggaucg ucggacugcu ccuuacaacu gcaauggccg cggaaguuac gcgccgaggu      120

uccgcuuacu auauguaccu ggaucguaau gacgccggcg aggcaauuuc uuuccccacc      180

acauugggga ugaacaaaug cuauauacag aucauggauu uaggacacau gugugacgcc      240

acuaugucau acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaua ccacaaguac cugggugguc uaugguacuu gucaucacaa gaaaggggag      360

gcuagaagga gccggcgcgc agugacacua ccgucccauu cuaccagaaa gcuccaaacg      420

aggucgcaga cuuggcugga gucacgggaa uacaccaagc accuuauucg cguugagaac      480

uggaucuuuc gaaaucccgg auucgcccug gcugccgcgg caauugccug guugcucggc      540

agcaguacau cccagaaagu gaucuaucug gucaugauac ugcucaucgc uccugccuac      600

ucuauuagau gcaucggcgu gagcaacagg gauuuuguag aagggauguc cggagguacu      660

uggguugaca uuguguuaga gcauggcggg ugugucaccg ugauggcaca ggauaagcca      720

acaguggaca ucgagcuggu caccacgacu guuucaaaua uggcugaagu gcggaguuac      780

ugcuaugagg ccagcauauc ugauauggcg uccgacucac guugucccac acaaggagaa      840

gccuaccuug acaaacagag cgauacccag uauguaugca agcgcacuuu gguggacaga      900

ggcuggggua acgggugugg acuguucggc aaaggcaguc uagucacaug cgcaaaguuu      960

gcuugcucua agaaaaugac cgggaagucc auucagccug agaaucucga auacaggauc     1020

augcugagcg ugcacggauc gcaacacucu gguaugauug uuaacgauac gggccaugag     1080

accgacgaga aucgggccaa aguggaaauc acaccaaacu caccccgagc ugaggccacu     1140

cuugggggau ucggcucccu ggguuuggau ugugaaccuc gcaccgggcu cgacuuuagu     1200

gaucuguauu acuuaacaau gaauaacaag cacuggcugg uccauaaaga gugguuccac     1260

gacaucccac ucccguggca cgcaggcgcc gauacuggaa caccccauug gaauaacaag     1320

gaagcucuug uggaguuuaa agacgcgcac gcaaagagac agaccguagu ugugcugggc     1380

agccaggagg gggccgucca uaccgcuuug gccggagcac uagaagccga gauggauggu     1440

gcuaagggca ggcuguccuc ugggcaccuc aaaugccggc ugaagaugga caaacuucgc     1500

cugaagggag ugagcuauuc auuguguacu gccgcauuca cguuuacaaa aauuccugcg     1560

gaaacccucc auggcacugu caccguggag guucaguacg cugguacaga cgggccaugc     1620

aaggugcccg cccaaauggc aguagauaug cagacccuga cuccugucgg cagauuaaua     1680

acagccaacc cagugaucac ggaaaguacc gaaaauucca agaugaugcu ggagcuagau     1740

cccccguucg gagacagcua cauuguuauc ggcguggggg agaaaaagau aacucaccac     1800

uggcauaggu cuggaucaac aauugguaag gcuuuugagg ccaccguccg uggcgcaaaa     1860

cggauggcug ugcucgggga cacagccugg gauuucggaa guguaggcgg ugcgcugaac     1920

agccuuggga aaggcaucca ccagaucuuc ggagccgcuu uuaaguccuu guucggcggg     1980

augucuuggu uuucgcaaau ucugaucgga acucuccuga uguggcuugg ccugaauacc     2040

aagaacggua gcauuuccuu gaugugucuc gcauuagggg gagugcugau auuccuguca     2100

acggccguua gugcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8348
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8348
gggagaaagc uuaccauggc cagaaaggag aaaaagaggc ggggcgcuga caccagcgug       60

gggaucgucg gacugcuccu uacaacugca auggccgcgg aaguuacgcg ccgagguucc      120

gcuuacuaua uguaccugga ucguaacgac gccggcgagg caauuucuuu ccccaccaca      180

uuggggauga auaaaugcua uauacagauc auggauuuag gacacaugug ugacgccacu      240

augucauacg aaugcccuau gcuggaugag ggcguggaac cagacgaugu agacuguugg      300

ugcaacacca caaguaccug gguggucuau gguacuuguc aucacaagaa aggggaggcu      360

agaaggagcc ggcgcgcagu gacacuaccg ucccauucua ccagaaagcu ccaaacgagg      420

ucgcagacuu ggcuggaguc acgggaauac accaagcacc uuauucgcgu ugagaauugg      480

aucuuucgaa accccggauu cgcccuggcu gccgcggcaa uugccugguu gcucggcagc      540

aguacauccc agaaagugau cuaucugguc augauacugc ucaucgcucc ugccuacucu      600

auuagaugca ucggcgugag caauagggau uuuguagaag ggauguccgg agguacuugg      660

guugacauug uguuagagca uggcgggugu gucaccguga uggcacagga uaagccaaca      720

guggacaucg agcuggucac cacgacuguu ucaaacaugg cugaagugcg gaguuacugc      780

uaugaggcca gcauaucuga uauggcgucc gacucacguu gucccacaca aggagaagcc      840

uaccuugaca aacagagcga uacccaguau guaugcaagc gcacuuuggu ggacagaggc      900

ugggguaaug gguguggacu guucggcaaa ggcagucuag ucacaugcgc aaaguuugcu      960

ugcucuaaga aaaugaccgg gaaguccauu cagccugaga accucgaaua caggaucaug     1020

cugagcgugc acggaucgca acacucuggu augauuguua augauacggg ccaugagacc     1080

gacgagaacc gggccaaagu ggaaaucaca ccaaauucac cccgagcuga ggccacucuu     1140

gggggauucg gcucccuggg uuuggauugu gaaccucgca ccgggcucga cuuuagugau     1200

cuguauuacu uaacaaugaa caauaagcac uggcuggucc auaaagagug guuccacgac     1260

aucccacucc cguggcacgc aggcgccgau acuggaacac cccauuggaa caauaaggaa     1320

gcucuugugg aguuuaaaga cgcgcacgca aagagacaga ccguaguugu gcugggcagc     1380

caggaggggg ccguccauac cgcuuuggcc ggagcacuag aagccgagau ggauggugcu     1440

aagggcaggc uguccucugg gcaccucaaa ugccggcuga agauggacaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcauucacgu uuacaaaaau uccugcggaa     1560

acccuccaug gcacugucac cguggagguu caguacgcug guacagacgg gccaugcaag     1620

gugcccgccc aaauggcagu agauaugcag acccugacuc cugucggcag auuaauaaca     1680

gccaacccag ugaucacgga aaguaccgag aacuccaaga ugaugcugga gcuagauccc     1740

ccguucggag acagcuacau uguuaucggc gugggggaga aaaagauaac ucaccacugg     1800

cauaggucug gaucaacaau ugguaaggcu uuugaagcca ccguccgugg cgcaaaacgg     1860

auggcugugc ucggggacac agccugggau uucggaagug uaggcggugc gcugaauagc     1920

cuugggaaag gcauccacca gaucuucgga gccgcuuuua aguccuuguu cggcgggaug     1980

ucuugguuuu cgcaaauucu gaucggaacu cuccugaugu ggcuuggccu gaacaccaag     2040

aaugguagca uuuccuugau gugucucgca uuagggggag ugcugauauu ccugucaacg     2100

gccguuagug cuugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8349
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8349
gggagaaagc uuaccaugag aaaggagaaa aagaggcggg gcgccgacac cagcgugggg       60

aucgucggac ugcuccuuac aacugcuaug gcagccgaag uuacgcgccg agguuccgcg      120

uacuauaugu accuggaucg uaacgacgcu ggcgaggcca uuucuuuccc caccacauug      180

gggaugaaua aaugcuauau acagaucaug gauuuaggac acauguguga cgcaacuaug      240

ucauacgaau gcccuaugcu ggaugagggc guggaaccag acgauguaga cuguuggugc      300

aacaccacaa guaccugggu ggucuauggu acuugucauc acaagaaagg ggaggccaga      360

aggagccggc gcgcugugac acuaccgucc cauucuacca gaaagcucca aacgaggucg      420

cagacuuggc uggagucacg ggaauacacc aagcaccuua uucgcguuga gaauuggauc      480

uuucgaaacc ccggauucgc acuggccgcu gccgcgauug caugguugcu cggcagcagu      540

acaucccaga aagugaucua ucuggucaug auacugcuca ucgccccugc uuacucuauu      600

agaugcaucg gcgugagcaa uagggauuuu guagaaggga uguccggagg uacuuggguu      660

gacauugugu uagagcaugg cggguguguc accgugaugg cccaggauaa gccaacagug      720

gacaucgagc uggucaccac gacuguuuca aacauggcag aagugcggag uuacugcuau      780

gaggcuagca uaucugauau ggccuccgac ucacguuguc ccacacaagg agaagcguac      840

cuugacaaac agagcgauac ccaguaugua ugcaagcgca cuuuggugga cagaggcugg      900

gguaaugggu guggacuguu cggcaaaggc agucuaguca caugcgccaa guuugcaugc      960

ucuaagaaaa ugaccgggaa guccauucag ccugagaacc ucgaauacag gaucaugcug     1020

agcgugcacg gaucgcaaca cucugguaug auuguuaaug auacgggcca ugagaccgac     1080

gagaaccggg cuaaagugga aaucacacca aauucacccc gagccgaggc uacucuuggg     1140

ggauucggcu cccuggguuu ggauugugaa ccucgcaccg ggcucgacuu uagugaucug     1200

uauuacuuaa caaugaacaa uaagcacugg cugguccaua aagagugguu ccacgacauc     1260

ccacucccgu ggcacgccgg cgcagauacu ggaacacccc auuggaacaa uaaggaagcc     1320

cuuguggagu uuaaagacgc ucacgcgaag agacagaccg uaguugugcu gggcagccag     1380

gagggggcag uccauaccgc cuuggcugga gcccuagaag cagagaugga uggugccaag     1440

ggcaggcugu ccucugggca ccucaaaugc cggcugaaga uggacaaacu ucgccugaag     1500

ggagugagcu auucauugug uacugcugcc uucacguuua caaaaauucc ugcagaaacc     1560

cuccauggca cugucaccgu ggagguucag uacgcgggua cagacgggcc augcaaggug     1620

cccgcucaaa uggccguaga uaugcagacc cugacuccug ucggcagauu aauaacagca     1680

aacccaguga ucacggaaag uaccgagaac uccaagauga ugcuggagcu agaucccccg     1740

uucggagaca gcuacauugu uaucggcgug ggggagaaaa agauaacuca ccacuggcau     1800

aggucuggau caacaauugg uaaagccuuu gaagcuaccg uccguggcgc caagcggaug     1860

gcagugcucg gggacacagc uugggauuuc ggaaguguag gcggugcccu gaauagccuu     1920

gggaaaggca uccaccagau cuucggagcg gccuuuaagu ccuuguucgg cgggaugucu     1980

ugguuuucgc aaauucugau cggaacucuc cugauguggc uuggccugaa caccaagaau     2040

gguagcauuu ccuugaugug ucucgcuuua gggggagugc ugauauuccu gucaacggca     2100

guuagugccu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8350
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8350
gggagaaagc uuaccaugaa ggagaaaaag agaaggggcg ccgacaccag cguggggauc       60

gucggacugc uccuuacaac ugcuauggca gccgaaguua cgcggcgcgg uuccgcguac      120

uauauguacc uggaucgaaa cgacgcuggc gaggccauuu cuuuccccac cacauugggg      180

augaauaaau gcuauauaca gaucauggau uuaggacaca ugugugacgc aacuauguca      240

uacgaaugcc cuaugcugga ugagggcgug gaaccagacg auguagacug uuggugcaac      300

accacaagua ccuggguggu cuaugguacu ugucaucaca agaaagggga ggcccguaga      360

agcaggcggg cugugacacu accgucccau ucuacccgca agcuccaaac gagaucgcag      420

acuuggcugg agucaaggga auacaccaag caccuuauuc ggguugagaa uuggaucuuu      480

cgcaaccccg gauucgcacu ggccgcugcc gcgauugcau gguugcucgg cagcaguaca      540

ucccagaaag ugaucuaucu ggucaugaua cugcucaucg ccccugcuua cucuauucga      600

ugcaucggcg ugagcaauag agauuuugua gaagggaugu ccggagguac uuggguugac      660

auuguguuag agcauggcgg gugugucacc gugauggccc aggauaagcc aacaguggac      720

aucgagcugg ucaccacgac uguuucaaac auggcagaag ugaggaguua cugcuaugag      780

gcuagcauau cugauauggc cuccgacuca cgguguccca cacaaggaga agcguaccuu      840

gacaaacaga gcgauaccca guauguaugc aagcguacuu ugguggaccg cggcuggggu      900

aaugggugug gacuguucgg caaaggcagu cuagucacau gcgccaaguu ugcaugcucu      960

aagaaaauga ccgggaaguc cauucagccu gagaaccucg aauacagaau caugcugagc     1020

gugcacggau cgcaacacuc ugguaugauu guuaaugaua cgggccauga gaccgacgag     1080

aacagggcua aaguggaaau cacaccaaau ucaccccggg ccgaggcuac ucuuggggga     1140

uucggcuccc uggguuugga uugugaaccu cgaaccgggc ucgacuuuag ugaucuguau     1200

uacuuaacaa ugaacaauaa gcacuggcug guccauaaag agugguucca cgacauccca     1260

cucccguggc acgccggcgc agauacugga acaccccauu ggaacaauaa ggaagcccuu     1320

guggaguuua aagacgcuca cgcgaagcgc cagaccguag uugugcuggg cagccaggag     1380

ggggcagucc auaccgccuu ggcuggagcc cuagaagcag agauggaugg ugccaagggc     1440

agacuguccu cugggcaccu caaaugcagg cugaagaugg acaaacuucg gcugaaggga     1500

gugagcuauu cauuguguac ugcugccuuc acguuuacaa aaauuccugc agaaacccuc     1560

cauggcacug ucaccgugga gguucaguac gcggguacag acgggccaug caaggugccc     1620

gcucaaaugg ccguagauau gcagacccug acuccugucg gccgcuuaau aacagcaaac     1680

ccagugauca cggaaaguac cgagaacucc aagaugaugc uggagcuaga ucccccguuc     1740

ggagacagcu acauuguuau cggcgugggg gagaaaaaga uaacucacca cuggcauaga     1800

ucuggaucaa caauugguaa agccuuugaa gcuaccguca ggggcgccaa gcguauggca     1860

gugcucgggg acacagcuug ggauuucgga aguguaggcg gugcccugaa uagccuuggg     1920

aaaggcaucc accagaucuu cggagcggcc uuuaaguccu uguucggcgg gaugucuugg     1980

uuuucgcaaa uucugaucgg aacucuccug auguggcuug gccugaacac caagaauggu     2040

agcauuuccu ugaugugucu cgcuuuaggg ggagugcuga uauuccuguc aacggcaguu     2100

agugccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8351
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8351
gggagaaagc uuaccaugga gaagaaaaga aggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc uuacaacugc uauggcagcc gaaguuacgc ggcgcgguuc cgcguacuau      120

auguaccugg aucgaaacga cgcuggcgag gccauuucuu uccccaccac auuggggaug      180

aauaagugcu auauacagau cauggauuua ggacacaugu gugacgcaac uaugucauac      240

gaaugcccua ugcuggauga gggcguggaa ccagacgaug uagacuguug gugcaacacc      300

acaaguaccu ggguggucua ugguacuugu caucacaaaa agggggaggc ccguagaagc      360

aggcgggcug ugacacuacc gucccauucu acccgcaaac uccaaacgag aucgcagacu      420

uggcuggagu caagggaaua caccaagcac cuuauucggg uugagaauug gaucuuucgc      480

aaccccggau ucgcacuggc cgcugccgcg auugcauggu ugcucggcag caguacaucc      540

cagaagguga ucuaucuggu caugauacug cucaucgccc cugcuuacuc uauucgaugc      600

aucggcguga gcaauagaga uuuuguagaa gggauguccg gagguacuug gguugacauu      660

guguuagagc auggcgggug ugucaccgug auggcccagg auaaaccaac aguggacauc      720

gagcugguca ccacgacugu uucaaacaug gcagaaguga ggaguuacug cuaugaggcu      780

agcauaucug auauggccuc cgacucacgg ugucccacac aaggagaagc guaccuugac      840

aagcagagcg auacccagua uguaugcaaa cguacuuugg uggaccgcgg cugggguaau      900

ggguguggac uguucggcaa gggcagucua gucacaugcg ccaaauuugc augcucuaag      960

aagaugaccg ggaaauccau ucagccugag aaccucgaau acagaaucau gcugagcgug     1020

cacggaucgc aacacucugg uaugauuguu aaugauacgg gccaugagac cgacgagaac     1080

agggcuaagg uggaaaucac accaaauuca ccccgggccg aggcuacucu ugggggauuc     1140

ggcucccugg guuuggauug ugaaccucga accgggcucg acuuuaguga ucuguauuac     1200

uuaacaauga acaauaaaca cuggcugguc cauaaggagu gguuccacga caucccacuc     1260

ccguggcacg ccggcgcaga uacuggaaca ccccauugga acaauaaaga agcccuugug     1320

gaguuuaagg acgcucacgc gaaacgccag accguaguug ugcugggcag ccaggagggg     1380

gcaguccaua ccgccuuggc uggagcccua gaagcagaga uggauggugc caagggcaga     1440

cuguccucug ggcaccucaa gugcaggcug aaaauggaca agcugcggcu gaaaggagug     1500

agcuauucau uguguacugc ugccuucacg uuuacaaaga uuccugcaga aacccuccau     1560

ggcacuguca ccguggaggu ucaguacgcg gguacagacg ggccaugcaa agugcccgcu     1620

caaauggccg uagauaugca gacccugacu ccugucggcc gcuuaauaac agcaaaccca     1680

gugaucacgg aaaguaccga gaacuccaag augaugcugg agcuagaucc cccguucgga     1740

gacagcuaca uuguuaucgg cgugggggaa aagaaaauaa cucaccacug gcauagaucu     1800

ggaucaacaa uugguaaggc cuuugaggcu accgucaggg gcgccaaacg uauggcagug     1860

cucggggaca cagcuuggga uuucggaagu guaggcggug cccugaauag ccuugggaag     1920

ggcauccacc agaucuucgg agcggccuuu aaauccuugu ucggcgggau gucuugguuu     1980

ucgcaaauuc ugaucggaac ucuccugaug uggcuuggcc ugaacaccaa gaaugguagc     2040

auuuccuuga ugugucucgc ucuuggggga guguuaauau uccugucaac ggcaguuagu     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8352
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8352
gggagaaagc uuaccaugaa gaaaagaagg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuua caacugcuau ggcagccgag guuacgcggc gcgguuccgc guacuauaug      120

uaccuggauc gaaacgacgc uggcgaagcc auuucuuucc ccaccacauu ggggaugaau      180

aagugcuaua uacagaucau ggauuuagga cacaugugug acgcaacuau gucauacgag      240

ugcccuaugc uggaugaagg cguggagcca gacgauguag acuguuggug caacaccaca      300

aguaccuggg uggucuaugg uacuugucau cacaaaaagg gggaagcccg uagaagcagg      360

cgggcuguga cacuaccguc ccauucuacc cgcaaacucc aaacgagauc gcagacuugg      420

cuggagucaa gggaguacac caagcaccuu auucggguug aaaauuggau cuuucgcaac      480

cccggauucg cacuggccgc ugccgcgauu gcaugguugc ucggcagcag uacaucccag      540

aaggugaucu aucuggucau gauacugcuc aucgccccug cuuacucuau ucgaugcauc      600

ggcgugagca auagagauuu uguagagggg auguccggag guacuugggu ugacauugug      660

uuagaacaug gcgggugugu caccgugaug gcccaggaua aaccaacagu ggacaucgag      720

cuggucacca cgacuguuuc aaacauggca gaggugagga guuacugcua ugaagcuagc      780

auaucugaua uggccuccga cucacggugu cccacacaag gagaggcgua ccuugacaag      840

cagagcgaua cccaguaugu augcaaacgu acuuuggugg accgcggcug ggguaauggg      900

uguggacugu ucggcaaggg cagucuaguc acaugcgcca aauuugcaug cucuaagaag      960

augaccggga aauccauuca gccugaaaac cucgaguaca gaaucaugcu gagcgugcac     1020

ggaucgcaac acucugguau gauuguuaau gauacgggcc augaaaccga cgagaacagg     1080

gcuaaggugg agaucacacc aaauucaccc cgggccgaag cuacucuugg gggauucggc     1140

ucccuggguu uggauuguga gccucgaacc gggcucgacu uuagugaucu guauuacuua     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgacau cccacucccg     1260

uggcacgccg gcgcagauac uggaacaccc cauuggaaca auaaagaggc ccuuguggaa     1320

uuuaaggacg cucacgcgaa acgccagacc guaguugugc ugggcagcca ggagggggca     1380

guccauaccg ccuuggcugg agcccuagag gcagaaaugg auggugccaa gggcagacug     1440

uccucugggc accucaagug caggcugaaa auggacaagc ugcggcugaa aggagugagc     1500

uauucauugu guacugcugc cuucacguuu acaaagauuc cugcagagac ccuccauggc     1560

acugucaccg uggaaguuca guacgcgggu acagacgggc caugcaaagu gcccgcucaa     1620

auggccguag auaugcagac ccugacuccu gucggccgcu uaauaacagc aaacccagug     1680

aucacggaga guaccgaaaa cuccaagaug augcuggagc uagauccccc guucggagac     1740

agcuacauug uuaucggcgu gggggagaag aaaauaacuc accacuggca uagaucugga     1800

ucaacaauug guaaggccuu ugaagcuacc gucaggggcg ccaaacguau ggcagugcuc     1860

ggggacacag cuugggauuu cggaagugua ggcggugccc ugaauagccu ugggaagggc     1920

auccaccaga ucuucggagc ggccuuuaaa uccuuguucg gcgggauguc uugguuuucg     1980

caaauucuga ucggaacucu ccugaugugg cuuggccuga acaccaagaa ugguagcauu     2040

uccuugaugu gucucgcucu ugggggagug uuaauauucc ugucaacggc aguuagugcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8353
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8353
gggagaaagc uuaccaugaa gagaaggggc gccgacacca gcguggggau cgucggacug       60

cuccuuacaa cugcuauggc agccgagguu acgcggcgcg guuccgcgua cuauauguac      120

cuggaucgaa acgacgcugg cgaagccauu ucuuucccca ccacauuggg gaugaauaaa      180

ugcuauauac agaucaugga uuuaggacac augugugacg caacuauguc auacgagugc      240

ccuaugcugg augaaggcgu ggagccagac gauguagacu guuggugcaa caccacaagu      300

accugggugg ucuaugguac uugucaucac aagaaagggg aagcccguag aagcaggcgg      360

gcugugacac uaccguccca uucuacccgc aagcuccaaa cgagaucgca gacuuggcug      420

gagucaaggg aguacaccaa acaccuuauu cggguugaaa auuggaucuu ucgcaacccc      480

ggauucgcac uggccgcugc cgcgauugca ugguugcucg gcagcaguac aucccagaag      540

gugaucuauc uggucaugau acugcucauc gccccugcuu acucuauucg augcaucggc      600

gugagcaaua gagauuuugu agaggggaug uccggaggua cuuggguuga cauuguguua      660

gaacauggcg ggugugucac cgugauggcc caggauaagc caacagugga caucgagcug      720

gucaccacga cuguuucaaa cauggcagag gugaggaguu acugcuauga agcuagcaua      780

ucugauaugg ccuccgacuc acgguguccc acacaaggag aggcguaccu ugacaaacag      840

agcgauaccc aguauguaug caagcguacu uugguggacc gcggcugggg uaaugggugu      900

ggacuguucg gcaaaggcag ucuagucaca ugcgccaagu uugcaugcuc uaaaaagaug      960

accgggaagu ccauucagcc ugaaaaccuc gaguacagaa ucaugcugag cgugcacgga     1020

ucgcaacacu cugguaugau uguuaaugau acgggccaug aaaccgacga gaacagggcu     1080

aaaguggaga ucacaccaaa uucaccccgg gccgaagcua cucuuggggg auucggcucc     1140

cuggguuugg auugugagcc ucgaaccggg cucgacuuua gugaucugua uuacuuaaca     1200

augaacaaua agcacuggcu gguccauaaa gaaugguucc acgacauccc acucccgugg     1260

cacgccggcg cagauacugg aacaccccau uggaacaaua aggaggcccu uguggaauuu     1320

aaagacgcuc acgcgaagcg ccagaccgua guugugcugg gcagccagga gggggcaguc     1380

cauaccgccu uggcuggagc ccuagaggca gaaauggaug gugccaaagg cagacugucc     1440

ucugggcacc ucaagugcag gcugaagaug gacaaacuuc ggcugaaggg agugagcuau     1500

ucauugugua cugcugccuu cacguuuaca aaaauuccug cagagacccu ccauggcacu     1560

gucaccgugg aaguucagua cgcggguaca gacgggccau gcaaggugcc cgcucaaaug     1620

gccguagaua ugcagacccu gacuccuguc ggccgcuuaa uaacagcaaa cccagugauc     1680

acggagagua ccgaaaacuc caaaaugaug cuggagcuag aucccccguu cggagacagc     1740

uacauuguua ucggcguggg ggagaagaag auaacucacc acuggcauag aucuggauca     1800

acaauuggua aagccuuuga agcuaccguc aggggcgcca agcguauggc agugcucggg     1860

gacacagcuu gggauuucgg aaguguaggc ggugcccuga auagccuugg gaaaggcauc     1920

caccagaucu ucggagcggc cuuuaagucc uuguucggcg ggaugucuug guuuucgcaa     1980

auucugaucg gaacucuccu gauguggcuu ggccugaaca ccaaaaaugg uagcauuucc     2040

uugauguguc ucgcuuuagg gggagugcug auauuccugu caacggcagu uagugccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8354
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8354
gggagaaagc uuaccaugag aaggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cuuacaacug cuauggcagc cgagguuacg cggcgcgguu ccgcguacua uauguaccug      120

gaucgaaacg acgcuggcga agccauuucu uuccccacca cauuggggau gaauaagugc      180

uauauacaga ucauggauuu aggacacaug ugugacgcaa cuaugucaua cgagugcccu      240

augcuggaug aaggcgugga gccagacgau guagacuguu ggugcaacac cacaaguacc      300

uggguggucu augguacuug ucaucacaaa aagggggaag cccguagaag caggcgggcu      360

gugacacuac cgucccauuc uacccgcaaa cuccaaacga gaucgcagac uuggcuggag      420

ucaagggagu acaccaagca ccuuauucgg guugaaaauu ggaucuuucg caaccccgga      480

uucgcacugg ccgcugccgc gauugcaugg uugcucggca gcaguacauc ccagaaagug      540

aucuaucugg ucaugauacu gcucaucgcc ccugcuuacu cuauucgaug caucggcgug      600

agcaauagag auuuuguaga ggggaugucc ggagguacuu ggguugacau uguguuagaa      660

cauggcgggu gugucaccgu gauggcccag gauaagccaa caguggacau cgagcugguc      720

accacgacug uuucaaacau ggcagaggug aggaguuacu gcuaugaagc uagcauaucu      780

gauauggccu ccgacucacg gugucccaca caaggagagg cguaccuuga caagcagagc      840

gauacccagu auguaugcaa acguacuuug guggaccgcg gcugggguaa ugggugugga      900

cuguucggca agggcagucu agucacaugc gccaaauuug caugcucuaa gaaaaugacc      960

gggaagucca uucagccuga aaaccucgag uacagaauca ugcugagcgu gcacggaucg     1020

caacacucug guaugauugu uaaugauacg ggccaugaaa ccgacgagaa cagggcuaag     1080

guggagauca caccaaauuc accccgggcc gaagcuacuc uugggggauu cggcucccug     1140

gguuuggauu gugagccucg aaccgggcuc gacuuuagug aucuguauua cuuaacaaug     1200

aacaauaaac acuggcuggu ccauaaggaa ugguuccacg acaucccacu cccguggcac     1260

gccggcgcag auacuggaac accccauugg aacaauaaag aggcccuugu ggaauuuaag     1320

gacgcucacg cgaaacgcca gaccguaguu gugcugggca gccaggaggg ggcaguccau     1380

accgccuugg cuggagcccu agaggcagaa auggauggug ccaagggcag acuguccucu     1440

gggcaccuca aaugcaggcu gaagauggac aagcugcggc ugaaaggagu gagcuauuca     1500

uuguguacug cugccuucac guuuacaaag auuccugcag agacccucca uggcacuguc     1560

accguggaag uucaguacgc ggguacagac gggccaugca aagugcccgc ucaaauggcc     1620

guagauaugc agacccugac uccugucggc cgcuuaauaa cagcaaaccc agugaucacg     1680

gagaguaccg aaaacuccaa gaugaugcug gagcuagauc ccccguucgg agacagcuac     1740

auuguuaucg gcguggggga gaaaaagaua acucaccacu ggcauagauc uggaucaaca     1800

auugguaagg ccuuugaagc uaccgucagg ggcgccaaac guauggcagu gcucggggac     1860

acagcuuggg auuucggaag uguaggcggu gcccugaaua gccuugggaa gggcauccac     1920

cagaucuucg gagcggccuu uaaauccuug uucggcggga ugucuugguu uucgcaaauu     1980

cugaucggaa cucuccugau guggcuuggc cugaacacca agaaugguag cauuuccuug     2040

augugucucg cucuuggggg aguguuaaua uuccugucaa cggcaguuag ugccugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8355
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8355
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccga gguuacgagg cgggguuccg cguacuauau guaccuggau      120

cgcaacgacg cuggcgaagc cauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgcaacua ugucauacga gugcccuaug      240

cuggaugaag gcguggagcc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaagccc gacguagcag aagggcugug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaguca      420

agagaguaca ccaagcaccu uauuaggguu gaaaauugga ucuuucggaa ccccggauuc      480

gcacuggccg cugccgcgau ugcaugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgccccu gcuuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaggg gauguccgga gguacuuggg uugacauugu guuagaacau      660

ggcgggugug ucaccgugau ggcccaggau aagccaacag uggacaucga gcuggucacc      720

acgacuguuu caaacauggc agaggugaga aguuacugcu augaagcuag cauaucugau      780

auggccuccg acucaaggug ucccacacaa ggagaggcgu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgcc aaauuugcau gcucuaagaa aaugaccggg      960

aaguccauuc agccugaaaa ccucgaguac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugaaaccg acgagaacag agcuaaggug     1080

gagaucacac caaauucccc cagggccgaa gcuacucuug ggggauucgg cucacugggu     1140

uuggauugug agccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggaaugg uuccacgaca ucccacuccc guggcacgcc     1260

ggcgcagaua cuggaacacc ccauuggaac aauaaagagg cccuugugga auuuaaggac     1320

gcucacgcga aacgacagac cguaguugug cugggcagcc aggagggggc aguccauacc     1380

gccuuggcug gagcccuaga ggcagaaaug gauggugcca agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugcug ccuucacguu uacaaagauu ccugcagaga cccuccaugg cacugucacc     1560

guggaaguuc aguacgcggg uacagacggg ccaugcaaag ugcccgcuca aauggccgua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag caaacccagu gaucacggag     1680

aguaccgaaa acuccaagau gaugcuggag cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggccu uugaagcuac cgucagaggc gccaaaagga uggcagugcu cggggacaca     1860

gcuugggauu ucggaagugu aggcggugcc cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag cggccuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcuc uugggggagu guuaauauuc cugucaacgg caguuagugc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8356
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8356
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccuuaca       60

acugcuaugg cagccgaggu uacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

aacgacgcug gcgaagccau uucuuucccc accacauugg ggaugaauaa gugcuauaua      180

cagaucaugg auuuaggaca caugugugac gcaacuaugu cauacgagug cccuaugcug      240

gaugaaggcg uggagccaga cgauguagac uguuggugca acaccacaag uaccugggug      300

gucuauggua cuugucauca caaaaagggg gaagcccgcc gaagccguag agcugugaca      360

cuaccguccc auucuaccag gaaacuccaa acgcggucgc agacuuggcu ggagucacgc      420

gaguacacca agcaccuuau uagaguugaa aauuggaucu uuaggaaccc cggauucgca      480

cuggccgcug ccgcgauugc augguugcuc ggcagcagua caucccagaa agugaucuau      540

cuggucauga uacugcucau cgccccugcu uacucuauuc ggugcaucgg cgugagcaau      600

cgcgauuuug uagaggggau guccggaggu acuuggguug acauuguguu agaacauggc      660

ggguguguca ccgugauggc ccaggauaag ccaacagugg acaucgagcu ggucaccacg      720

acuguuucaa acauggcaga ggugcgaagu uacugcuaug aagcuagcau aucugauaug      780

gccuccgacu caagaugucc cacacaagga gaggcguacc uugacaagca gagcgauacc      840

caguauguau gcaaaaggac uuugguggac cggggcuggg guaaugggug uggacuguuc      900

ggcaagggca gucuagucac augcgccaaa uuugcaugcu cuaagaaaau gaccgggaag      960

uccauucagc cugaaaaccu cgaguaccgu aucaugcuga gcgugcacgg aucgcaacac     1020

ucugguauga uuguuaauga uacgggccau gaaaccgacg agaaccgcgc uaagguggag     1080

aucacaccaa auucccccag agccgaagcu acucuugggg gauucggcuc acuggguuug     1140

gauugugagc cuaggaccgg gcucgacuuu agugaucugu auuacuuaac aaugaacaau     1200

aaacacuggc ugguccauaa ggaaugguuc cacgacaucc cacucccgug gcacgccggc     1260

gcagauacug gaacacccca uuggaacaau aaagaggccc uuguggaauu uaaggacgcu     1320

cacgcgaaac ggcagaccgu aguugugcug ggcagccagg agggggcagu ccauaccgcc     1380

uuggcuggag cccuagaggc agaaauggau ggugccaagg gccgacuguc cucugggcac     1440

cucaaaugcc gccugaagau ggacaagcug agacugaaag gagugagcua uucauugugu     1500

acugcugccu ucacguuuac aaagauuccu gcagagaccc uccauggcac ugucaccgug     1560

gaaguucagu acgcggguac agacgggcca ugcaaagugc ccgcucaaau ggccguagau     1620

augcagaccc ugacuccugu cggcagguua auaacagcaa acccagugau cacggagagu     1680

accgaaaacu ccaagaugau gcuggagcua gaucccccgu ucggagacag cuacauuguu     1740

aucggcgugg gggagaaaaa gauaacucac cacuggcauc ggucuggauc aacaauuggu     1800

aaggccuuug aagcuaccgu ccgcggcgcc aaaagaaugg cagugcucgg ggacacagcu     1860

ugggauuucg gaaguguagg cggugcccug aauagccuug ggaagggcau ccaccagauc     1920

uucggagcgg ccuuuaaauc cuuguucggc gggaugucuu gguuuucgca aauucugauc     1980

ggaacucucc ugauguggcu uggccugaac accaagaaug guagcauuuc cuugaugugu     2040

cucgcucuug ggggaguguu aauauuccug ucaacggcag uuagugccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8357
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8357
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccuuacaacu       60

gcuauggcag ccgagguuac gagaagggga uccgcguacu auauguaccu ggaucggaac      120

gacgcuggug aagccauuuc uuuccccacc acauugggca ugaauaagug cuauauacag      180

aucauggauu uagggcacau gugugacgca acuaugucau acgagugccc uaugcuggau      240

gaaggagugg agccagacga uguagacugu uggugcaaca ccacaaguac cugggugguc      300

uauggcacuu gucaucacaa aaagggugaa gcccgccgaa gccguagagc ugugacacua      360

ccgucccauu cuaccaggaa acuccaaacg cggucgcaga cuuggcugga gucacgcgag      420

uacaccaagc accuuauuag aguugaaaau uggaucuuua ggaaccccgg guucgcacug      480

gccgcugccg cgauugcaug guugcucgga agcaguacau cccagaaagu gaucuaucug      540

gucaugauac ugcucaucgc cccugcuuac ucuauucggu gcaucggcgu gagcaaucgc      600

gauuuuguag agggcauguc cgggggaacu uggguugaca uuguguuaga acaugguggc      660

ugugucaccg ugauggccca ggauaagcca acaguggaca ucgagcuggu caccacgacu      720

guuucaaaca uggcagaggu gcgaaguuac ugcuaugaag cuagcauauc ugauauggcc      780

uccgacucaa gaugucccac acaaggggag gcguaccuug acaagcagag cgauacccag      840

uauguaugca aaaggacuuu gguggaccgg ggauggggca augguugugg gcuguucgga      900

aagggcaguc uagucacaug cgccaaauuu gcaugcucua agaaaaugac cggcaagucc      960

auucagccug aaaaccucga guaccguauc augcugagcg ugcacggguc gcaacacucu     1020

ggaaugauug uuaaugauac gggucaugaa accgacgaga accgcgcuaa gguggagauc     1080

acaccaaauu cccccagagc cgaagcuacu cuuggcgggu ucggaucacu gggcuuggau     1140

ugugagccua ggaccggucu cgacuuuagu gaucuguauu acuuaacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gacaucccac ucccguggca cgccggggca     1260

gauacuggca caccccauug gaacaauaaa gaggcccuug uggaauuuaa ggacgcucac     1320

gcgaaacggc agaccguagu ugugcuggga agccaggagg gcgcagucca uaccgccuug     1380

gcuggggccc uagaggcaga aauggaugga gccaaggguc gacuguccuc uggccaccuc     1440

aaaugccgcc ugaagaugga caaacuuaga cugaaggggg ugagcuauuc auuguguacu     1500

gcugccuuca cguuuacaaa gauuccugca gagacccucc auggaacugu caccguggaa     1560

guucaguacg cgggcacaga cgguccaugc aaagugcccg cucaaauggc cguagauaug     1620

cagacccuga cuccugucgg gagguuaaua acagcaaacc cagugaucac ggagaguacc     1680

gaaaacucca agaugaugcu ggagcuagau cccccguucg gcgacagcua cauuguuauc     1740

ggagugggcg agaaaaagau aacucaccac uggcaucggu cugggucaac aauuggaaag     1800

gccuuugaag cuaccguccg cggugccaaa agaauggcag ugcucggcga cacagcuugg     1860

gauuucggga guguaggagg cgcccugaau agccuuggua aggggaucca ccagaucuuc     1920

ggcgcggccu uuaaauccuu guucggaggc augucuuggu uuucgcaaau ucugaucggg     1980

acucuccuga uguggcuugg acugaacacc aagaauggca gcauuuccuu gaugugucuc     2040

gcucugggug ggguguuaau auuccuguca acggcaguua gugccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  8358
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8358
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu uacaacugcc       60

auggcugcag agguuacgag aaggggaucc gccuacuaua uguaccugga ucggaacgac      120

gcgggugaag cuauuucuuu ccccaccaca uugggcauga auaagugcua uauacagauc      180

auggauuuag ggcacaugug ugacgccacu augucauacg agugcccuau gcuggaugaa      240

ggaguggagc cagacgaugu agacuguugg ugcaacacca caaguaccug gguggucuau      300

ggcacuuguc aucacaaaaa gggugaagca cgccgaagcc guagagccgu gacacuaccg      360

ucccauucua ccaggaaacu ccaaacgcgg ucgcagacuu ggcuggaguc acgcgaguac      420

accaagcacc uuauuagagu ugaaaauugg aucuuuagga accccggguu cgcucuggca      480

gccgcugcca uugcgugguu gcucggaagc aguacauccc agaaagugau cuaucugguc      540

augauacugc ucaucgcacc cgccuacucu auucggugca ucggcgugag caaucgcgau      600

uuuguagagg gcauguccgg gggaacuugg guugacauug uguuagaaca ugguggcugu      660

gucaccguga uggcucagga uaagccaaca guggacaucg agcuggucac cacgacuguu      720

ucaaacaugg ccgaggugcg aaguuacugc uaugaagcaa gcauaucuga uauggcuucc      780

gacucaagau gucccacaca aggggaggcc uaccuugaca agcagagcga uacccaguau      840

guaugcaaaa ggacuuuggu ggaccgggga uggggcaaug guugugggcu guucggaaag      900

ggcagucuag ucacaugcgc gaaauuugcc ugcucuaaga aaaugaccgg caaguccauu      960

cagccugaaa accucgagua ccguaucaug cugagcgugc acgggucgca acacucugga     1020

augauuguua augauacggg ucaugaaacc gacgagaacc gcgcaaaggu ggagaucaca     1080

ccaaauuccc ccagagcuga agccacucuu ggcggguucg gaucacuggg cuuggauugu     1140

gagccuagga ccggucucga cuuuagugau cuguauuacu uaacaaugaa caauaaacac     1200

uggcuggucc auaaggaaug guuccacgac aucccacucc cguggcacgc uggggccgau     1260

acuggcaccc cucauuggaa caauaaagag gcacuugugg aauuuaagga cgcccacgcu     1320

aaacggcaga caguaguugu gcugggaagc caggagggcg cgguccauac cgcauuggcc     1380

ggggcucuag aggccgaaau ggauggagca aagggucgac uguccucugg ccaccucaaa     1440

ugccgccuga agauggacaa acuuagacug aaggggguga gcuauucauu guguacugcc     1500

gcuuucacgu uuacaaagau uccugccgag acccuccaug gaacugucac cguggaaguu     1560

caguacgcag gcacagacgg uccaugcaaa gugcccgcgc aaauggcugu agauaugcag     1620

acccugacuc cugucgggag guuaauaaca gccaacccag ugaucacgga gaguaccgaa     1680

aacuccaaga ugaugcugga gcuagauccc ccguucggcg acagcuacau uguuaucgga     1740

gugggcgaga aaaagauaac ucaccacugg caucggucug ggucaacaau uggaaaggca     1800

uuugaagcca ccguccgcgg ugcuaaaaga auggccgugc ucggcgacac agcaugggau     1860

uucgggagug uaggaggcgc ucugaauagc cuugguaagg ggauccacca gaucuucggc     1920

gccgcguuua aauccuuguu cggaggcaug ucuugguuuu cgcaaauucu gaucgggacu     1980

cuccugaugu ggcuuggacu gaacaccaag aauggcagca uuuccuugau gugucucgcc     2040

cugggugggg uguuaauauu ccugucaacg gcuguuagug caugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8359
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  8359
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8360
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8360
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagccg uuacgagaag gggauccgcg uacuauaugu accuggaccg gaacgaugcu      120

ggugaggcca uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcaacuaug ucauacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaggcccgc cgaagccgua gagcugugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggaaucacg cgaguacacc      420

aagcaccuua uuagaguuga gaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagaaggca uguccggggg aacuuggguu gauauugugu uagagcaugg uggcuguguc      660

accgugaugg cccaggacaa gccaacagug gauaucgaac uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaggcuagca uaucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaagcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugagaacc ucgaauaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugagaccgau gaaaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaggc uacucuuggc ggguucggau cacugggcuu ggacugugaa     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggagugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacacu     1260

ggcacacccc auuggaacaa uaaagaagcc cuuguggagu uuaaggaugc ucacgcgaaa     1320

cggcagaccg uaguugugcu gggaagccag gaaggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagagaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagaaacc cuccauggaa cugucaccgu ggagguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaggcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8361
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8361
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccuu       60

acaacugcua uggcagccgc gguuacgagg cgggguuccg cuuacuauau guaccuggau      120

cgcaacgacg ccggcgaggc aauuucuuuc cccaccacau uggggaugaa uaagugcuau      180

auacagauca uggauuuagg acacaugugu gacgccacua ugucauacga augcccuaug      240

cuggaugagg gcguggaacc agacgaugua gacuguuggu gcaacaccac aaguaccugg      300

guggucuaug guacuuguca ucacaaaaag ggggaggcuc gacguagcag aagggcagug      360

acacuaccgu cccauucuac ccggaaacuc caaacgcgcu cgcagacuug gcuggaauca      420

agagaguaca ccaagcaccu uauuaggguu gagaauugga ucuuucggaa ccccggauuc      480

gcccuggcug ccgcggcaau ugccugguug cucggcagca guacauccca gaaagugauc      540

uaucugguca ugauacugcu caucgcuccu gccuacucua uucgcugcau cggcgugagc      600

aaucgagauu uuguagaagg gauguccgga gguacuuggg uugacauugu guuagagcau      660

ggcgggugug ucaccgugau ggcacaggau aagccaacag uggacaucga acuggucacc      720

acgacuguuu caaacauggc ugaggugaga aguuacugcu augaggccag cauaucugau      780

auggcguccg acucaaggug ucccacacaa ggagaagccu accuugacaa gcagagcgau      840

acccaguaug uaugcaaacg gacuuuggug gaccguggcu gggguaaugg guguggacug      900

uucggcaagg gcagucuagu cacaugcgca aaauuugcuu gcucuaagaa aaugaccggg      960

aaguccauuc agccugagaa ccucgaauac cgcaucaugc ugagcgugca cggaucgcaa     1020

cacucuggua ugauuguuaa ugauacgggc caugagaccg acgaaaacag agccaaggug     1080

gagaucacac caaauucccc cagggcugag gccacucuug ggggauucgg cucacugggu     1140

uuggauugug aaccucggac cgggcucgac uuuagugauc uguauuacuu aacaaugaac     1200

aauaaacacu ggcuggucca uaaggagugg uuccacgaca ucccacuccc guggcacgca     1260

ggcgccgaua cuggaacacc ccauuggaac aauaaagaag cucuugugga guuuaaggac     1320

gcgcacgcaa aacgacagac cguaguugug cugggcagcc aggaaggggc cguccauacc     1380

gcuuuggccg gagcacuaga ggccgagaug gauggugcua agggccgccu guccucuggg     1440

caccucaaau gcagacugaa gauggacaag cugaggcuga aaggagugag cuauucauug     1500

uguacugccg cauucacguu uacaaagauu ccugcggaaa cccuccaugg cacugucacc     1560

guggagguuc aguacgcugg uacagacggg ccaugcaaag ugcccgccca aauggcagua     1620

gauaugcaga cccugacucc ugucggccgg uuaauaacag ccaacccagu gaucacggaa     1680

aguaccgaga acuccaagau gaugcuggaa cuagaucccc cguucggaga cagcuacauu     1740

guuaucggcg ugggggagaa aaagauaacu caccacuggc aucgcucugg aucaacaauu     1800

gguaaggcuu uugaggccac cgucagaggc gcaaaaagga uggcugugcu cggggacaca     1860

gccugggauu ucggaagugu aggcggugcg cugaauagcc uugggaaggg cauccaccag     1920

aucuucggag ccgcuuuuaa auccuuguuc ggcgggaugu cuugguuuuc gcaaauucug     1980

aucggaacuc uccugaugug gcuuggccug aacaccaaga augguagcau uuccuugaug     2040

ugucucgcac uugggggagu guuaauauuc cugucaacgg ccguuagugc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8362
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8362
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagguguuac ucgacguggc      120

ucagccuacu auauguaccu ggauagaaac gacgcugggg aggcaauuag uuuucccacg      180

acccuaggaa ugaauaaaug cuauauacaa aucauggauc ucggccauau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggugugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugggaccu gucaccauaa gaaaggagag      360

gcgaggcggu cucgcagagc ugugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg cuuugcccug gcagccgcug caauugccug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc uccugccuac      600

uccauuaggu gcaucggggu gucaaaucgg gauuucguag aaggaaugag ugguggcaca      660

uggguugaca uuguguugga gcacggggga ugugucacug ugauggcgca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggcagaggu gcguucuuac      780

ugcuaugagg ccuccauauc agauauggcu agcgacaguc gcugucccac ucagggcgaa      840

gccuaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

gguuggggga auggaugugg ccuguuuggc aaaggguccc uugucacuug cgcaaaguuc      960

gcuugcagca aaaagaugac aggaaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacgguuc ccagcauuca ggcaugauug uuaaugauac cgggcacgag     1080

acggacgaaa accgagccaa aguggagauc accccaaaua guccccgcgc ggaggccaca     1140

cucggaggcu uugguagccu gggguuagau ugugaaccua gaacuggccu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcaggagcu gauacaggca cuccccacug gaacaauaag     1320

gaagcccuag uggaguucaa agacgcucau gccaagaggc agaccguagu ugugcugggg     1380

ucucaggaag gagcagucca cacagcccuc gcuggugcgc uggaggcaga gauggauggc     1440

gccaaagggc ggcuuagcuc aggacaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggcg ugaguuauuc cuuauguacc gcugccuuua cuuucacgaa aauuccugca     1560

gaaacacugc acgguaccgu cacuguggag guucaauacg ccgggaccga cggcccaugc     1620

aaggugcccg cucagauggc cguagauaug cagacacuaa ccccugucgg aaggcucaua     1680

acugcaaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gcgacucaua cauuguuauc ggggugggag agaagaagau aacccaccau     1800

uggcaccgua gugguagcac uauuggcaaa gcguucgagg cuacaguccg aggggccaag     1860

cggauggcag uguugggaga caccgccugg gauuucggcu ccguaggugg ggcucucaau     1920

ucucugggca aaggaaucca ucaaaucuuu ggcgccgcau ucaagucgcu guuuggggga     1980

augagcuggu ucucccagau ucuuaucggc acacuguuga uguggcucgg ucugaacacu     2040

aaaaaugggu caauuaguuu aaugugucug gcucuaggag gcgugcucau auuucugucu     2100

accgccguua gcgcgugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8363
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8363
gggagaaagc uuaccaugag aggccugagc uccaggaagc ggcgcucuca cgacgugcuc       60

accguccagu uccuuauccu ggggauguug uuaaugacag gagccguuac ucgacguggu      120

ucagcuuacu auauguaccu ggauagaaac gacgcaggcg aggccauuag uuuucccacg      180

acccuaggga ugaauaaaug cuauauacaa aucauggauc ucggacauau gugugacgcg      240

acaaugagcu acgaaugccc uaugcuggau gagggcgugg aaccagacga uguagacugu      300

uggugcaaca cuaccuccac augggugguc uaugguaccu gucaccauaa gaaaggggag      360

gcuaggcggu cucgcagagc cgugacucuu ccgucgcacu caacaaggaa gcugcagacc      420

cggagccaga cgugguugga aagucgcgag uacacuaaac aucucauucg aguugagaau      480

uggaucuuca gaaaccccgg auuugcacug gccgcugcag ccauugcuug gcugcucggc      540

uccucuacca gccagaaggu gaucuauuua gucaugauac ugcuuaucgc cccugcguac      600

uccauuaggu gcaucggcgu gucaaaucgg gauuucguag aagggaugag uggagguaca      660

uggguugaca uuguguugga gcacggcggg ugugucacug ugauggcaca agauaagcca      720

accguggaca ucgaacuggu cacaaccacg guuagcaaca uggccgaggu gcguucuuac      780

ugcuaugagg cuuccauauc agauauggcc agcgacaguc gcugucccac ucagggagaa      840

gcauaccuag acaaacaguc ugauacacag uauguaugca agagaacccu cguggacagg      900

ggcuggggua augggugugg acuguuuggc aaaggcuccc uugucacuug cgcuaaguuc      960

gccugcagca aaaagaugac agggaagucg auucaaccug agaaccugga auaccggauc     1020

auguugucug ugcacggauc ccagcauuca gguaugauug uuaaugauac cggccacgag     1080

acggacgaaa accgagcgaa aguggagauc accccaaaua guccccgcgc cgaggcaaca     1140

cucgggggau uuggcagccu ggguuuagau ugugaaccua gaacugggcu ggacuucucc     1200

gaucucuauu accuuaccau gaacaauaag cauuggcugg uccacaaaga gugguuucac     1260

gacaucccau ugccguggca ugcuggcgcc gauacaggaa cuccccacug gaacaauaag     1320

gaagcucuag uggaguucaa agacgcccau gcaaagaggc agaccguagu ugugcugggc     1380

ucucaggaag gggccgucca cacagcucuc gcgggagcac uggaggccga gauggauggu     1440

gcuaaaggcc ggcuuagcuc agggcaucug aagugccgcu ugaagaugga caaacucaga     1500

cugaagggag ugaguuauuc cuuauguacc gccgcauuua cuuucacgaa aauuccugcc     1560

gaaacacugc acggcaccgu cacuguggag guucaauacg cugguaccga cgggccaugc     1620

aaggugcccg cccagauggc aguagauaug cagacacuaa ccccugucgg caggcucaua     1680

acugcgaacc cagugaucac agaaagcacg gagaacucua aaaugaugcu ggaacuugau     1740

cccccguuug gagacucaua cauuguuauc ggcguggggg agaagaagau aacccaccau     1800

uggcaccgua guggaagcac uauugguaag gcuuucgagg ccacaguccg gggcgcaaaa     1860

cgaauggccg uguuggggga caccgcuugg gauuucggau ccguaggcgg ugcccucaau     1920

ucucugggga aaggcaucca ucaaaucuuu ggagcagcuu ucaagucgcu guuuggcggg     1980

augagcuggu ucucccagau ucuuaucgga acacuguuga uguggcucgg ccugaacacu     2040

aaaaaugguu caauuaguuu aaugugucug gcccuagggg gagugcucau auuucugucu     2100

accgcgguua gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8364
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 3 opt

<400>  8364
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacau aguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8365
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 3 opt

<400>  8365
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacacu      240

ugugacgcca ccaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac aaccaguacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacccuac cgucccauuc uacgaggaag      420

cuccaaacuc ggucgcagac cuggcuggag ucacgcgaau acacaaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacuuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcacag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccacc      840

caaggagaag ccuaccuuga caaacagagc gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugaccugc      960

gcaaaguuug cuugcucuaa gaaaaugacg gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cagacgagaa ucgagccaaa gucgaaauca cuccaaacuc accccgcgcu     1140

gaggccaccc uugggggauu cggcucccug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagug aucuguauua cuuaacuaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auaccggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau acugcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacgg ccgcauucac auuuaccaaa     1560

auaccugcgg aaacucucca uggcaccgug acaguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacucugac accugugggc     1680

agguuaauca cggccaaccc aguuauuacc gaaaguacug aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acacaccacu ggcaucguuc uggaucaacc aucgguaagg cuuuugaggc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acugccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaaugcaa agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa cggcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8366
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 3 opt

<400>  8366
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugg cuacgaaacc     1080

gaugaggacc gagcaaaggu cgaggugaca ccuaauucac cacgcgcuga agccacucuu     1140

gggggauucg gcagucuggg uuuggauugu gagcccagaa ccgggcucga cuuuagcgau     1200

cuguauuacu uaacaaugaa caauaaacac uggcugguac auaaggaaug guuccacgac     1260

auaccucucc cauggcacgc uggcgccgau acuggaaccc cgcauuggaa caauaaagag     1320

gcacuuguug aauuuaagga cgcccacgcu aagaggcaga caguggucgu gcugggcucc     1380

caggaggggg cgguccauac cgcauuggcc ggagcucuag aggccgaaau ggauggugca     1440

aaaggccggc uguucucugg gcaccucaag ugccgccuga aaauggacaa acuuagacug     1500

aagggaguga gcuauucauu guguacugcc gcuuuuacgu ucacaaaggu ucccgccgag     1560

acccuccaug gcacugugac cguagaaguc caguacgcag guacagacgg gccuugcaag     1620

aucccagugc aaauggcggu ugauaugcag acccugacuc ccgugggcag guuaauuaca     1680

gcuaacccug ucaucacgga gaguaccgaa aauuccaaaa ugaugcugga gcuagaccca     1740

cccuuuggag auagcuacau agugauuggc guaggggaca agaaaaucac ucaccacugg     1800

caucguucug gaucaacaau cgguaaggcc uucgaggcaa ccgugcgggg cgccaaacga     1860

auggcuguuc ucggggauac agccugggac uucggaagug ucggcggugu guuuaacagc     1920

cuggggaagg gcauucacca gaucuucgga gcagcuuuua agucccuuuu cggcgggaug     1980

ucuugguuuu cgcaaauuuu gauaggaacu cugcucgugu ggcugggccu uaacaccaaa     2040

aaugguagca ucucccugac guguuuggcc cucgggggag ucaugauuuu ccugucaacc     2100

gcgguuagug ccgauguggg cugcucugug gacuuuagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8367
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 3 opt

<400>  8367
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8368
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 3 opt

<400>  8368
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugu caaugauacc     1080

ggcuacgaaa cagacgagaa ccgagcaaag guggagguaa cuccuaauuc accacgcgcu     1140

gaagccaccc uugggggauu cggcagucug gguuuggauu gugagcccag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaaac acuggcuggu ucauaaggaa     1260

ugguuccacg acauaccucu cccauggcac gcuggcgccg auaccggaac accgcauugg     1320

aacaauaaag aggcacuugu ggaauuuaag gacgcccacg cuaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcggugcau acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggcaa gcuguucucu gggcaccuca aaugccggcu gaagauggac     1500

aaacuucgcc ugaagggagu uagcuauuca uuguguacgg ccgcuuuuac auucaccaag     1560

gugcccgccg agacucucca uggcaccgua acagucgaag ugcaguacgc agguaccgac     1620

gggccuugca aaaucccagu ucaaauggcg guggauaugc agacucugac acccgucggc     1680

agauuaauua cggcuaaccc ugugaucacc gagaguacug aaaacuccaa gaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auaguaauug gcguggggga caaaaagauc     1800

acacaccacu ggcauagguc uggaucaacc aucgguaaag ccuucgaggc aacaguucgu     1860

ggcgccaagc ggauggcugu ccucggggau acugccuggg acuucggaag ugugggcggu     1920

guguuuaaua gccuggggaa gggcauucac cagaucuucg gagcagcuuu uaaaucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugauaggaa cccugcucgu cuggcugggc     2040

cuuaacacga agaaugguag caucucccug accuguuugg cccucggggg aguuaugauu     2100

uuccugucaa cagcggugag ugccgaugug ggcugcucug uagacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8369
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 3 opt

<400>  8369
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucacacugg uggcugucgc caucgcgugg      540

uugcucggca guuccacuuc ucagaaggug auuuaccugg uaaugauccu gcucauugca      600

cccgccuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguaccu gggucgacgu gguguuagaa cacggcgggu gugucacagu uauggcucag      720

gauaaaccua ccguggacau ugagcuggua acgacuacag ugaguaacau ggccgagguc      780

cguagcuacu gcuacgaagc aucuaucucc gauauggcuu cagacagccg cuguccaacc      840

caaggagagg ccuaucuuga caagcagagu gauacucagu acgugugcaa aagaacauug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugaccugc      960

gcgaaguuua cgugcuccaa aaagaugacc gggaaaagca uucagcccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu caacauuccg guaugaucgu gaaugaugaa     1080

aaccgagcca agguagaggu uacaccuaau ucaccacgcg cagaggcuac ucuuggcggg     1140

uucggaaguc ugggcuugga cugugaaccc agaaccgguc ucgauuuuag cgaccuguac     1200

uauuuaacaa ugaacaauaa acacuggcug gugcauaagg agugguucca cgauauuccu     1260

cucccauggc acgccggggc ugacacuggc accccgcauu ggaacaauaa agaagcccuu     1320

gucgaguuua aggaugcaca cgccaagagg cagacagugg ucgugcuggg aucccaggaa     1380

ggcgcuguuc auaccgcguu ggcaggggcc cuagaggcug agauggacgg agccaaaggu     1440

cggcuguucu cuggccaccu caagugccgc cugaaaaugg auaaacuuag acugaagggg     1500

gugagcuacu cauuguguac ugcagccuuu acguucacaa agguacccgc ugaaacccuc     1560

cauggaacug ucaccgugga gguucaguau gccggcacag acgguccuug caaggugcca     1620

gcacaaaugg cggucgacau gcagacccug acucccgugg ggagguuaau aacagcuaac     1680

ccuguaauca cggaaaguac cgagaacucc aaaaugaugc uggaacuaga uccacccuuu     1740

ggcgacagcu acauugugau cggaguuggc gauaagaaaa uaacucacca cuggcaucgu     1800

ucugggucaa caauuggaaa ggccuucgag gcaaccgucc ggggugccaa acgaauggcu     1860

gugcucggcg acacagccug ggauuucggg agugugggag gcgucuuuaa uagccugggu     1920

aaggggaucc accagaucuu cggcgcagcu uuuaaguccc uuuucggagg caugucuugg     1980

uuuucgcaaa uuuugaucgg gacucugcuc guuuggcugg gacuuaacac caaaaauggc     2040

agcauuuccc ugacguguuu ggcccucggu ggggugauga uauuccuguc aaccgcggug     2100

agugccgacg uaggaugcuc ugucgauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8370
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 3 opt

<400>  8370
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccggaag      360

aaaggggaag cacgcagauc caggcgggcc gucacacuac caucucauuc gacccgcaag      420

cuccaaacgc gaucacagac uuggcuggag agcagagagu auaccaagca ccuuauaaaa      480

guggaaaauu ggaucuuuag gaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauacggug cauuggcgua uccaaucgug auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cauggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cgcagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagcag auguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aaggacuuug      900

guggaccggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucgaauca ugcugucggu gcacggaucu caacacuccg guaugauugu caaugauacc     1080

ggcuacgaaa cagacgagaa ccgcgcaaag guggagguaa cuccuaauuc accaagagcu     1140

gaagccaccc uugggggauu cggcagucug gguuuggauu gugagcccag gacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaaac auuggcuggu ucacaaggaa     1260

ugguuccaug acauaccucu cccauggcac gcuggcgccg auaccggaac accgcacugg     1320

aacaauaaag aggcacuugu ggaauuuaag gacgcccaug cuaagcggca gaccgucgug     1380

guccugggcu cccaggaggg ggcggugcac acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggcaa gcuguucucu gggcaucuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu uagcuauuca uuguguacgg ccgcuuuuac auucaccaag     1560

gugcccgccg agacucucca cggcaccgua acagucgaag ugcaguacgc agguaccgac     1620

gggccuugca aaaucccagu ucaaauggcg guggauaugc agacucugac acccgucggc     1680

agguuaauua cggcuaaccc ugugaucacc gagaguacug aaaacuccaa gaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auaguaauug gcguggggga caaaaagauc     1800

acacaucacu ggcaccguuc uggaucaacc aucgguaaag ccuucgaggc aacaguucgg     1860

ggcgccaagc gaauggcugu ccucggggau acugccuggg acuucggaag ugugggcggu     1920

guguuuaaua gccuggggaa gggcauucau cagaucuucg gagcagcuuu uaaaucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugauaggaa cccugcucgu cuggcugggc     2040

cuuaacacga agaaugguag caucucccug accuguuugg cccucggggg aguuaugauu     2100

uuccugucaa cagcggugag ugccgaugug ggcugcucug uagacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8371
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 3 opt

<400>  8371
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugaccgu caaugauauu     1080

ggcuacgaaa cagacgagaa ccgagcaaag guggagguaa cuccuaauuc accacgcgcu     1140

gaagccaccc uugggggauu cggcagucug gguuuggauu gugagcccag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaaac acuggcuggu ucauaaggaa     1260

ugguuccacg acauaccucu cccauggcac gcuggcgccg auaccggaac accgcauugg     1320

aacaauaaag aggcacuugu ggaauuuaag gacgcccacg cuaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcggugcau acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggcaa gcuguucucu gggcaccuca aaugccggcu gaagauggac     1500

aaacuucgcc ugaagggagu uagcuauuca uuguguacgg ccgcuuuuac auucaccaag     1560

gugcccgccg agacucucca uggcaccgua acagucgaag ugcaguacgc agguaccgac     1620

gggccuugca aaaucccagu ucaaauggcg guggauaugc agacucugac acccgucggc     1680

agauuaauua cggcuaaccc ugugaucacc gagaguacug aaaacuccaa gaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auaguaauug gcguggggga caaaaagauc     1800

acacaccacu ggcauagguc uggaucaacc aucgguaaag ccuucgaggc aacaguucgu     1860

ggcgccaagc ggauggcugu ccucggggau acugccuggg acuucggaag ugugggcggu     1920

guguuuaaua gccuggggaa gggcauucac cagaucuucg gagcagcuuu uaaaucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugauaggaa cccugcucgu cuggcugggc     2040

cuuaacacga agaaugguag caucucccug accuguuugg cccucggggg aguuaugauu     2100

uuccugucaa cagcggugag ugccgaugug ggcugcucug uagacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8372
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 3 opt

<400>  8372
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugg cuacgaaacc     1080

gaugagaauc gagcaaaggu cgaggugaca ccuaacucac cacgcgcuga agccacucuu     1140

gggggauucg gcagucuggg uuuggacugu gagcccagaa ccgggcucga uuuuagcgac     1200

cuguauuacu uaacaaugaa uaacaaacac uggcugguac auaaggaaug guuccacgau     1260

auaccucucc cauggcacgc uggcgccgac acuggaaccc cgcauuggaa uaacaaagag     1320

gcacuuguug aauuuaagga ugcccacgcu aagaggcaga caguggucgu gcugggcucc     1380

caggaggggg cgguccauac cgcauuggcc ggagcucuag aggccgaaau ggacggugca     1440

aaaggcaagc uguucucugg gcaccucaaa ugccggcuga agauggauaa acuucgccug     1500

aagggaguga gcuauucauu guguacugcc gcuuuuacgu ucacaaaggu ucccgccgag     1560

acccuccaug gcacugugac cguagaaguc caguacgcag guacagacgg gccuugcaaa     1620

aucccagugc aaauggcggu ugacaugcag acccugacuc ccgugggcag auuaauuaca     1680

gcuaauccug ucaucacgga gaguaccgaa aacuccaaga ugaugcugga gcuagaucca     1740

cccuuuggag acagcuacau agugauuggc guaggggaua aaaagaucac ucaccacugg     1800

cauaggucug gaucaacaau cgguaaagcc uucgaggcaa ccgugcgugg cgccaagcgg     1860

auggcuguuc ucggggacac agccugggau uucggaagug ucggcggugu guuuaacagc     1920

cuggggaagg gcauucacca gaucuucgga gcagcuuuua aaucccuuuu cggcgggaug     1980

ucuugguuuu cgcaaauuuu gauaggaacu cugcucgugu ggcugggccu uaauaccaag     2040

aacgguagca ucucccugac guguuuggcc cucgggggag ucaugauuuu ccugucaacc     2100

gcgguuagug ccgacguggg cugcucugug gauuuuagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8373
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 3 opt

<400>  8373
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggag aggcauccac cagauuuucg gagccgcuuu uaaauccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8374
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 3 opt

<400>  8374
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacgcaagcg uggggauagu cggacugcuc cuuaccacag ccauggcggc ugaaguuacu      120

cgacgugguu ccgccuacua uauguaccug gauagaaaug acgcaggcga ggccaucucu      180

uuccccacga ccuuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcua caaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac uaccaguaca uggguggucu augguaccug ucaucacaag      360

aaaggggagg caaggcggag ccgcagagcc gugacucuac cgucccauuc uacaaggaag      420

cuccaaaccc ggucgcagac guggcuggag ucacgcgaau acacuaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcucugg ccgcggcagc cauagcuugg      540

uugcucggca gcaguaccuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcauacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacau ggguugacgu ggucuuagag cauggcgggu gugugacugu gauggcucag      720

gauaagccaa ccgucgacau agagcugguu acaaccacgg ugucaaauau ggccgaagua      780

cguaguuacu gcuaugaggc gagcauuucu gauauggccu ccgacucacg cugucccacu      840

caaggagaag cauaccuuga caaacagagc gauacacagu augugugcaa gagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacuugc      960

gcuaaguuug ccugcucuaa gaaaaugaca gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cggacgagaa ucgagcuaaa gucgaaauca ccccaaacuc accccgcgcc     1140

gaggcaacac uugggggauu cggcucccug gguuuggauu gugaaccuag aacugggcuc     1200

gacuuuagug aucuguauua cuuaaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcug auacaggaac uccccauugg     1320

aauaacaagg aagcgcuugu agaguuuaaa gacgcacacg ccaagaggca gaccguugug     1380

guccugggca gccaggaggg ggcugugcau acagccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguaccg cagcguucac uuuuacgaaa     1560

auaccugcug aaacacucca uggcaccgug acuguugagg ugcaguacgc cgguaccgac     1620

gggccaugca agguacccgc acaaauggcc gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugcuaaccc aguuauuaca gaaaguacgg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaaag ccuuugaggc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggaag ugugggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa ccgcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8375
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 3 opt

<400>  8375
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga cuggccuugg cugccgcggc aauagccugg      540

cuccugggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu cuuaaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuucguaga agggaugucc      660

ggagguacuu ggguugacgu gguccuggag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcuuguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuacuugga caaacagagc gauacccagu augugugcaa gagaacucug      900

gucgacaggg gcugggguaa cgggugugga cuauuuggca aaggcagucu cgugacaugc      960

gcaaaguucg cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucuggaa     1020

uaccggauua ugcuuagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc ugggaggguu uggcuccuug ggucucgauu gugaaccuag aaccgggcug     1200

gacuucagug auuuauauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuucacg acauuccacu uccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuggu agaguucaaa gacgcgcacg caaagaggca gaccguugug     1380

gucuugggca gccaggaggg ggccgugcau accgcucuag ccggagcacu ggaagccgag     1440

auggauggug cuaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggac     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug ccgcauuuac guucacaaaa     1560

auaccugcgg aaacccugca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agaccuuaac uccugugggc     1680

aggcugauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc ccccguuugg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuucgaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acugggggac acagccuggg auuuuggaag ugugggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagauuuucg gagccgcuuu caagucccug     1980

uuuggcggga ugucuugguu cucgcaaauc cucauuggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauaucccuc augugucugg cauuaggggg aguucugauc     2100

uuucuaucaa cggccgucag ugcugacgug ggcugcucug uggauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8376
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 3 opt

<400>  8376
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu gguaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccau cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8377
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 3 opt

<400>  8377
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaaauu      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8378
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 3 opt

<400>  8378
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacuggc ccaaauggca gucgauaugc agaccuuaac ucccgugggc     1680

aggcugauca cagccaaccc uguuauuacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgacc cacccuucgg agauagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acugggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagauuuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uucgcaaauc cucauuggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauaucccuc augugucugg cauuaggggg aguucugauc     2100

uuccuaucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8379
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 3 opt

<400>  8379
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu acccuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac aaccaguacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacccuac cgucccauuc uacgaggaag      420

cuccaaacuc ggucgcagac cuggcuggag ucacgcgaau acacaaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacuuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcacag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccacc      840

caaggagaag ccuaccuuga caaacagagc gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugaccugc      960

gcaaaguuug cuugcucuaa gaaaaugacg gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cagacgagaa ucgagccaaa gucgaaauca cuccaaacuc accccgcgcu     1140

gaggccaccc uugggggauu cggcucccug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagug aucuguauua cuuaacuaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auaccggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau acugcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacgg ccgcauucac auuuaccaaa     1560

auaccugcgg aaacucucca uggcaccgug acaguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacucugac accugugggc     1680

agguuaauca cggccaaccc aguuauuacc gaaaguacug aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acacaccacu ggcaucguuc uggaucaacc aucgguaagg cuuuugaggc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acugccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaauacga agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa ccgccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8380
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 3 opt

<400>  8380
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggucgugag ugccgacgug ggcugcucug ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8381
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 3 opt

<400>  8381
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaagugcu uggguggucu augguaccug ucaucacaag      360

aaaggggagg caaggcggag ccgcagagcc gugacucuac cgucccauuc uacaaggaag      420

cuccaaaccc ggccccagac guggcuggag ucgcgcgaau acacuaagca ccuuauucga      480

guugagaacu ggaucuuuag aaauccugga uucgcucugg ccgcggcagc cauagcuugg      540

uugcucggcu caagcaccag ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccagcauacu ccauuaggug caucggcgug ucuaaccggg auuuuguaga agggaugagc      660

ggagguacau ggguugacgu ggucuuagag cauggcgggu gugugacugu gauggcucag      720

gauaagccca ccgucgacau agagcugguu acaaccacgg uguccaauau ggccgaagua      780

cguucauacu gcuaugaggc gaguauuagc gauauggccu cugacucccg cuguccuacu      840

caaggagaag cauaccuuga caaacaguca gauacacagu augugugcaa gagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagccu agugacuugc      960

gcuaaguuug ccugcaguaa gaaaaugaca gggaagucua uccagccaga gaaucucgaa     1020

uaccggauua ugcuguccgu ucacggaagc caacacucgg guaugaucgu gaacgauacc     1080

ggccaugaga cggacgagaa ucgagcuaaa gucgaaauca cccccaacuc uccucgcgcc     1140

gaggcaacac uugggggauu cggcucccug gguuuggauu gugaaccaag aacugggcuc     1200

gacuuuucag aucuguauua cuuaaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccgcu ccccuggcac gccggcgcug auacaggaac uccucauugg     1320

aauaacaagg aagcgcuugu agaguuuaaa gacgcacacg ccaagaggca gaccguugug     1380

guccugggca gucaggaggg ggcugugcau acagccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguaccg cagcguucac uuuuacgaaa     1560

auaccagcug aaacacucca uggcaccgug acuguugagg ugcaguacgc cgguaccgac     1620

gggcccugca agguaccugc acaaauggcc gucgauaugc agacacugac cccagugggc     1680

agguuaauca cugcuaaccc cguuauuaca gaaucaacgg agaauaguaa gaugaugcug     1740

gagcuagacc cgccuuucgg agauuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguag cggaucuacu aucgguaaag ccuuugaagc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggauc agugggcggu     1920

gcccugaaca gucuugggaa aggcauccac cagauuuucg gagcugcauu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguuc gaucagcuug augugucucg ccuuaggggg aguucugaua     2100

uuccugucca ccgcugucuc agccgacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8382
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 3 opt

<400>  8382
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc caaaaaggug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaaaccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

cagggagaag ccuaccuuga caagcaaagc gauacccagu augugugcaa aagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca agggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa aaagaugacc gggaaaucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg cagcacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaag gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaaac acuggcuggu gcauaaggag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaaag aagcucuugu agaguuuaag gacgcgcacg caaagaggca aaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaaaggccg gcuguccucu gggcaccuca agugccgccu gaaaauggac     1500

aagcugagac ugaaaggagu cagcuauuca uuguguacug ccgcauucac guuuacaaag     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccagauggca gucgauaugc aaacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa aaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaagaaaauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa gggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcagauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca aaaacgguag cauauccuug augugucucg cacuuggggg aguuuuaauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8383
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 3 opt

<400>  8383
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacaag gcagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu ccauucggug caucggcgug ucuaaccgug auuuuguaga agggaugagc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug uguccaauau ggcugaagua      780

cgcucauacu gcuaugaggc caguauuagc gauauggcgu cugacuccag augucccaca      840

caaggagaag ccuaccuuga caaacaguca gauacccagu augugugcaa gaggacuuug      900

gucgaccggg gcugggguaa cgggugugga cuguucggca aaggcagccu agugacaugc      960

gcaaaguuug cuugcaguaa gaaaaugacc gggaagucua uccagccuga gaaucucgaa     1020

uaccgaauua ugcuguccgu ucacggaagc caacacucgg guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgcgccaaa gucgaaauca caccaaacuc ucccagagcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag gaccgggcuc     1200

gacuuuucag aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagcggca gaccguugug     1380

guccugggca gucaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg ccugagcucc gggcaccuca aaugcagacu gaagauggac     1500

aaacuuaggc ugaagggagu cucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

cguuuaauca cagccaaccc aguuauuacg gaaucaaccg agaauaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucggag cggaucuaca aucgguaagg cuuuugaagc caccgugcga     1860

ggcgcaaaaa gaauggcugu acucggggac acagccuggg auuucggauc agugggcggu     1920

gcgcugaaca gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguuc gaucagcuug augugucucg cauuaggggg aguucugaua     2100

uuccugucca cggccgucuc agcugacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8384
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 3 opt

<400>  8384
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcagcgcu       60

gacaccuccg ugggcauagu cgggcugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacguggau cugcuuacua uauguaccug gauagaaaug acgccgguga ggcaaucuca      180

uuccccacca cauugggcau gaacaaaugc uauauucaga ucauggauuu agggcacaug      240

ugugacgcca cuaugaguua cgaaugcccu augcuggaug agggagugga accagacgau      300

guagacuguu ggugcaauac cacaagcacc uggguggucu auggcacuug ucaucacaag      360

aaaggugagg cuaggcgguc ccgcagagca gugacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccggg uucgcccugg cugccgcggc aauagccugg      540

uugcucggaa guuccacauc ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuaca gcauuaggug caucggcgug uccaaccggg auuuuguaga aggcauguca      660

gggggaacuu ggguugacgu ggucuuagag caugguggcu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugaguaauau ggcugaagua      780

cguagcuacu gcuaugaggc cucuauuucc gauauggcgu cagacagccg cugucccaca      840

caaggggaag ccuaccuuga caaacagagu gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gauggggcaa cgguuguggg cuguucggaa aaggcucucu agugacaugc      960

gcaaaguuug cuugcuccaa gaaaaugacc ggcaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucggu ucacgggucu caacacuccg gaaugaucgu gaacgauacg     1080

ggucaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uuggcggguu cggaagucug ggcuuggauu gugaaccuag aaccggucuc     1200

gacuuuagcg aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggggccg auacuggcac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggau cccaggaggg cgccgugcau accgcuuugg ccggggcacu agaagccgag     1440

auggauggag cuaagggucg gcugucuagc ggccaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggggu cucauauagu uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggaacugug accguugagg ugcaguacgc uggcacagac     1620

gguccaugca agguacccgc ccaaauggca gucgauaccc agacucugac accugugggg     1680

agguuaauca cggccaaccc aguuauuacc gaauccacug agaauagcaa gaugaugcug     1740

gagcuagauc ccccguucgg cgacucuuac aucgugauag gagucggcga gaaaaagauu     1800

acacaccacu ggcaucguuc agggaguacc aucggaaagg cuuuugaagc cacagugcgg     1860

ggugcaaaac gaauggcugu acucggcgac acugccuggg auuucgggag cgugggaggc     1920

gcgcugaacu cccuugguaa agggauccac cagauuuucg gcgccgcuuu uaagucuuug     1980

uucggaggca ugucgugguu uagccaaauc cugauuggga cccuccugau guggcuugga     2040

cugaauacga agaacggcuc cauaucauug augugucucg cacugggugg gguuuuaauc     2100

uuccugagua ccgccgucuc ugcugacgug ggaugcagcg uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8385
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 3 opt

<400>  8385
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccaaug uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacguggua gcgcuuacua uauguaccug gauagaaacg acgccggcga ggcaaucucc      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucuua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac cacaucaacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ucgcagagca gugacacuac cgagccauuc caccaggaag      420

cuccaaacgc ggucucagac uuggcuggag ucgcgcgaau acaccaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggcu caagcacaag ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu ccauuaggug caucggcgug ucuaaucggg auuuuguaga agggaugagc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug uguccaacau ggcugaagua      780

cguucauacu gcuaugaggc caguauuagc gauauggcgu cugacucccg cugucccaca      840

caaggagaag ccuaccuuga caaacaguca gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcagccu agugacaugc      960

gcaaaguuug cuugcaguaa gaaaaugacc gggaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu ucacggaagc caacacucgg guaugaucgu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagccaaa gucgaaauca caccaaauuc uccccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuucag aucuguauua cuuaacaaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aacaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gucaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaucc aguuauuacg gaaucaaccg agaacaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguag cggaucuaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcacgaa gaauggcugu acucggggac acagccuggg auuucggauc agugggcggu     1920

gcgcugaaua gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaacacca aaaaugguuc gaucagcuug augugucucg cauuaggggg aguucugaua     2100

uuccugucca cggccgucuc agcugacgug ggcugcagug uggauuuuuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8386
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 3 opt

<400>  8386
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gaaaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggagguuacg      120

cgacgugguu ccgcuuacua uauguaccug gacagaaaug augccggcga agcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggacuu aggacacaug      240

ugugaugcca cuaugucaua cgagugcccu augcuggacg aaggcgugga gccagaugac      300

guagauuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggaa ucacgcgagu acaccaagca ccuuauucga      480

guugaaaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg acuuuguaga ggggaugucc      660

ggagguacuu ggguugaugu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gacaagccaa cagucgauau agaacugguu accacgacug ugucaaauau ggcugaggua      780

cguaguuacu gcuaugaagc cagcauuucu gacauggcgu ccgauucacg cugucccaca      840

caaggagagg ccuaccuuga caaacagagc gacacccagu augugugcaa gagaacuuug      900

gucgauaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga aaaucucgag     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgacacg     1080

ggccaugaga ccgaugaaaa ucgagccaaa gucgagauca caccaaacuc accccgcgcu     1140

gaagccacuc uugggggauu cggcucccug gguuuggacu gugagccuag aaccgggcuc     1200

gauuuuagug accuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagaa     1260

ugguuccacg auauuccacu cccguggcac gcaggcgccg acacuggaac accccauugg     1320

aauaacaagg aggcucuugu agaguuuaaa gaugcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaagg ggccgugcau accgcuuugg ccggagcacu agaggccgaa     1440

auggacggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggau     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg agacccucca uggcacugug accguugaag ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgacaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gagaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggau acagccuggg acuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugaugug ggcugcucug uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8387
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 3 opt

<400>  8387
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuuuaaggug cauuggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu gguccuggag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau cgagcuuguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauaucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuacuugga caaacagagc gauacccagu augugugcaa gagaacucug      900

gucgacaggg gcugggguaa cgggugugga cuauucggca aaggcagucu cgugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucuggaa     1020

uaccggauca ugcuuagcgu ucacggaucg caacacucug guaugauugu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc ugggaggguu cggcuccuug ggucucgauu gugaaccuag aaccgggcug     1200

gacuuuagug auuuauauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acaucccacu uccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuggu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

gucuugggca gccaggaggg ggccgugcau accgcucuag ccggagcacu ggaagccgag     1440

auggauggug cuaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggac     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug ccgcauucac guuuacaaaa     1560

auuccugcgg aaacccugca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agaccuuaac uccugugggc     1680

aggcugauaa cagccaaccc aguuaucacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc ccccguucgg agacagcuac auugugaucg gcgucgggga gaaaaagaua     1800

acucaccacu ggcaucguuc uggaucaaca auugguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acugggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uucgcaaauu cucaucggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauuucccuc augugucugg cauuaggggg aguucugaua     2100

uuccuaucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8388
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 3 opt

<400>  8388
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccaaug uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacguggua gcgcuuacua uauguaccug gauagaaacg acgccggcga ggcaaucucc      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucuua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac cacaucaacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ucgcagagca gugacacuac cgagccauuc caccaggaag      420

cuccaaacgc ggucucagac uuggcuggag ucgcgcgaau acaccaagca ccuuauucga      480

guugagaauu ggauguuuag aaaccccgga uucgcccugg cugccgcggc aaucgccugg      540

uugcucggcu caagcacaag ucagaaagug auauaucugg ucaugauccu gcucauugcu      600

ccugccuacu ccaucaggug cauuggcgug ucuaaucggg auuuuguaga agggaugagc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau cgagcugguu accacgacug uguccaacau ggcugaagua      780

cguucauacu gcuaugaggc caguauaagc gauauggcgu cugacucccg cugucccaca      840

caaggagaag ccuaccuuga caaacaguca gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcagccu agugacaugc      960

gcaaaguuug cuugcaguaa gaaaaugacc gggaagucua uucagccuga gaaccucgaa     1020

uaccggauca ugcuguccgu ucacggaagc caacacucgg guaugauugu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagccaaa gucgaaauca caccaaauuc uccccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuucag aucuguauua cuuaacaaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acaucccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aacaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gucaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auuccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauaa cagccaaucc aguuaucacg gaaucaaccg agaacaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac auugugaucg gcgucgggga gaaaaagaua     1800

acucaccacu ggcaucguag cggaucuaca auugguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggauc agugggcggu     1920

gcgcugaaua gucuugggaa aggcauccac cagaucuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cucuccugau guggcuuggc     2040

cugaacacca agaaugguuc gauuagcuug augugucucg cauuaggggg aguucugaua     2100

uuccugucca cggccgucuc agcugacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8389
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 3 opt

<400>  8389
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggauccugag aaaucccgga uuugccuugg cugccgcggc aauagccugg      540

cuccugggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu cuuaaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuucguaga agggaugucc      660

ggagguacuu ggguugacgu gguccuggag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcuuguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuacuugga caaacagagc gauacccagu augugugcaa gagaacucug      900

gucgacaggg gcugggguaa cgggugugga cuauuuggca aaggcagucu cgugacaugc      960

gcaaaguucg cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucuggaa     1020

uaccggauua ugcuuagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc ugggaggguu uggcuccuug ggucucgauu gugaaccuag aaccgggcug     1200

gacuucagug auuuauauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuucacg acauuccacu uccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuggu agaguucaaa gacgcgcacg caaagaggca gaccguugug     1380

gucuugggca gccaggaggg ggccgugcau accgcucuag ccggagcacu ggaagccgag     1440

auggauggug cuaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggac     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug ccgcauuuac guucacaaaa     1560

auaccugcgg aaacccugca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agaccuuaac uccugugggc     1680

aggcugauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc ccccguuugg agacagcuac aucgugauag gcauugggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuucgaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcugggggac acagccuggg auuuuggaag uguaggcggu     1920

gcgcuuaaca gcuuggggaa aggcauucac cagaucuucg gagccgcuuu caagucccug     1980

uuuggcggga ugucuugguu cucgcaaauu cucauaggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag caucucccuc augugucugg cauuaggggg agugcugauu     2100

uuucuaucaa cggccguuag ugcugacguc ggcugcucug uggauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8390
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 3 opt

<400>  8390
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu ucaaccacgg ugaguaauau ggcugaagua      780

cguagcuacu gcuaugaggc cacuauuucu gauaucgcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccgccguu     1380

gugcugggca gccaggaggg ggcuguccau accgccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug cagcguucac guuuacaaaa     1560

aucccugcug aaacccucca uggcacuguc accguggagg uucaguacgc cgguacagac     1620

gggccaugca aggugcccgc acaaauggcc guagauaugc agacccugac uccugucggc     1680

agguuaauua cagcuaaccc agugaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaaag ccuuugaggc aaccguccgg     1860

ggcgcuaagc gaauggccgu gcucggggac acagcguggg auuucggaag uguaggcggu     1920

gcccugaaca gccuugggaa aggcauucac cagaucuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg ccuuaggggg agugcugauu     2100

uuccugucaa cggcuguuag ugccgacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8391
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 3 opt

<400>  8391
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacag cccccgcgcu     1140

gaggccacuc uugggggauu cggcucacug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca cccaggaggg ggccgugcau acugcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguacgg ccgcauucac auuuaccaaa     1560

auaccugcgg aaacucucca uggcaccgug acaguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacucugac accugugggc     1680

agguuaauca cggccaaccc aguuauuacc gaaucaacug agaauaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acacaccacu ggcaucguag cggaucuacc aucgguaagg cuuuugaagc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acugccuggg auuucggauc agugggcggu     1920

gcgcugaaca gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaauacga agaacgguuc gauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucca ccgccgucuc agcugacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8392
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 3 opt

<400>  8392
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagc ugcaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agaccuuaac uccugucggc     1680

aggcugauca cagccaaccc agugauuacg gaaaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc ccccguucgg agacagcuac aucguuauag gcguggggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccguccgg     1860

ggcgcaaaac gaauggcugu gcugggggac acagccuggg auuucggaag uguaggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagauuuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uucgcaaauc cucauuggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauaucccuc augugucugg cauuaggggg agugcugauc     2100

uuccuaucaa cggccguuag ugcugacguc ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8393
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 3 opt

<400>  8393
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gaaaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggagguuacg      120

cgacgugguu ccgcuuacua uauguaccug gacagaaaug augccggcga agcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggacuu aggacacaug      240

ugugaugcca cuaugucaua cgagugcccu augcuggacg aaggcgugga gccagaugac      300

guagauuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggaa ucacgcgagu acaccaagca ccuuauucga      480

aucgaaaacu ggauauuuag aaaucccgga uucgcccugg cugccgcggc aaucgccugg      540

uugcucggca gcaguacauc ccagaaaguu auuuaucugg ugaugauccu gcucauugcu      600

ccugccuacu cuaucaggug cauaggcguc agcaaccggg acuuugugga ggggaugucc      660

ggagguacuu ggguagaugu uguguuagag cauggcgggu gugucaccgu gauggcacag      720

gacaagccaa caguggauau ugaacugguc accacgacug uuucaaauau ggcugaggug      780

cguaguuacu gcuaugaagc cagcaucucu gacauggcgu ccgauucacg cugucccaca      840

caaggagagg ccuaccuuga caaacagagc gacacccagu auguaugcaa gagaacuuug      900

guggauaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga aaaucucgag     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgacacg     1080

ggccaugaga ccgaugaaaa ucgagccaaa guggagauua caccaaacuc accccgcgcu     1140

gaagccacuc uugggggauu cggcucccug gguuuggacu gugagccuag aaccgggcuc     1200

gauuuuagug accuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagaa     1260

ugguuccacg auauaccacu cccguggcac gcaggcgccg acacuggaac accccauugg     1320

aauaacaagg aggcucuugu ggaguuuaaa gaugcgcacg caaagaggca gaccguaguu     1380

gugcugggca gccaggaagg ggccguccau accgcuuugg ccggagcacu agaggccgaa     1440

auggacggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggau     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg agacccucca uggcacuguc accguggaag uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagacaugc agacccugac uccugucggc     1680

agguuaauua cagccaaccc agugaucacg gagaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auaguuauug gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggau acagccuggg acuucggaag uguaggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag caucuccuug augugucucg cauuaggggg agugcugauu     2100

uuccugucaa cggccguuag ugcugauguc ggcugcucug uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8394
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 3 opt

<400>  8394
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaaaguaucg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auagugauug gcgucgggga gaaaaagauc     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaagg cuuuugaggc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac accgccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguag caucuccuug augugucucg cauuaggggg aguucugauu     2100

uuccugucaa ccgccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8395
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 3 opt

<400>  8395
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug ucaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug cugccuucac guuuacaaaa     1560

auaccugcag aaacccucca uggcacuguu accguggagg uacaguacgc ggguacagac     1620

gggccaugca agguccccgc ucaaauggcc guggauaugc agacccugac uccuguuggc     1680

agguuaauca cagcaaaccc agugauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgucauag gcguggggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaaag ccuuugaggc uaccguacgg     1860

ggcgccaagc gaauggcagu gcucggggac acagcuuggg auuucggaag uguuggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcggccuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuuc auguguuugg cucucggggg aguccugauc     2100

uuuuuaucaa cggcagugag ugccgacgug ggcugcucug ucgauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8396
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 3 opt

<400>  8396
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggcaa acuguccucu gggcaccuca agugccggcu gaaaauggac     1500

aagcugcgcc ugaaaggagu cagcuauuca uuguguacug ccgcauucac guuuacaaag     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agauuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa aaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaagaaaauu     1800

acucaccacu ggcauagguc uggaucaaca aucgguaagg cuuuugaggc caccgugcgu     1860

ggcgcaaaac ggauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa gggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca aaaacgguag cauauccuug augugucucg cacuuggggg aguuuuaauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8397
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 3 opt

<400>  8397
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gaggcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaagg ggccgugcau accgcuuugg ccggagcacu agaggccgaa     1440

auggacggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggau     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg agacccucca uggcacugug accguugaag ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgacaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gagaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggau acagccuggg acuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugaugug ggcugcucug uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8398
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 3 opt

<400>  8398
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc uggugccgac     1620

gggccaugca agguacccgc acaaauggcc gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugcuaaccc aguuauuaca gaaaguacgg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaaag ccuuugaggc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggaag ugugggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa ccgcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8399
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 3 opt

<400>  8399
gggagaaagc uuaccaugag aaucauuaac gccggcaagg agaaaaagag gcgcggggcu       60

gacaccaaug ugggaauagu cggucugcuc cuuacaacug caauggccgc ggaaguuacg      120

cggcgaggca gcgcuuacua uauguaccug gaucguaacg acgccgggga ggcaaucucc      180

uuccccacca cauugggaau gaauaaaugc uauauucaga ucauggauuu aggccacaug      240

ugugacgcca cuaugucuua cgaaugcccu augcuggaug agggugugga accagacgau      300

guagacuguu ggugcaacac cacaucaacc uggguggucu augggacuug ucaucacaag      360

aaaggagagg cuagaaggag ucggcgcgca gugacacuac cgagccauuc caccagaaag      420

cuccaaacga ggucucagac uuggcuggag ucgcgggaau acaccaagca ccuuauucgc      480

guugagaauu ggaucuuucg aaaccccggc uucgcccugg cugccgcggc aauagccugg      540

uugcucggcu caagcacaag ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu ccauuagaug caucggggug ucuaauaggg auuuuguaga aggaaugagc      660

gguggcacuu ggguugacgu ggucuuagag caugggggau gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug uguccaacau ggcugaagua      780

cggucauacu gcuaugaggc caguauuagc gauauggcgu cugacucccg uugucccaca      840

caaggcgaag ccuaccuuga caaacaguca gauacccagu augugugcaa gcgcacuuug      900

gucgacagag guugggggaa uggauguggc cuguucggca aagggagccu agugacaugc      960

gcaaaguuug cuugcaguaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uacaggauua ugcuguccgu ucacgguagc caacacucgg gcaugaucgu gaaugauacg     1080

gggcaugaga ccgacgagaa ccgggccaaa gucgaaauca caccaaauuc uccccgagcu     1140

gaggccacuc uuggaggcuu cgguucccug ggguuggauu gugaaccucg caccggccuc     1200

gacuuuucag aucuguauua cuuaacaaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggagccg auacuggcac accccauugg     1320

aacaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagagaca gaccguugug     1380

guccugggga gucaggaggg agccgugcau accgcuuugg ccggugcacu agaagccgag     1440

auggauggcg cuaaggggag gcugagcucc ggacaccuca aaugccggcu gaagauggac     1500

aaacuucgcc ugaagggcgu cucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca ugguacugug accguugagg ugcaguacgc ugggacagac     1620

ggcccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccuguggga     1680

agauuaauca cagccaaucc aguuauuacg gaaucaaccg agaacaguaa gaugaugcug     1740

gagcuagauc ccccguucgg cgacuccuac aucgugauag gggucggaga gaaaaagauu     1800

acucaccacu ggcauaggag cgguucuaca aucggcaagg cuuuugaagc caccgugcgu     1860

ggggcacggc gaauggcugu acucggagac acagccuggg auuucggcuc aguggguggg     1920

gcgcugaaua gucuuggcaa aggaauccac cagauuuucg gcgccgcuuu uaagagcuug     1980

uucgggggaa uguccugguu uucucaaauc cugauuggca cucuccugau guggcuuggu     2040

cugaacacca aaaauggguc gaucagcuug augugucucg cacugggagg cguuuuaaua     2100

uuccugucca cggccgucuc agcugacgug gguugcagug uggauuuuuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8400
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 3 opt

<400>  8400
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gaaaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggagguuacg      120

cgacgugguu ccgcuuacua uauguaccug gacagaaaag augccggcga agcaaucucu      180

uuccccacca cauuggggau gaauaagugc uauauucaga ucauggacuu aggacacaug      240

ugugaugcca cuaugucaua cgagugcccu augcuggacg aaggcgugga gccagaugac      300

guagauuguu ggugcaacac cacaaguacc uggguggucu augguacuug ucaucacaaa      360

aagggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggaa ucacgcgagu acaccaaaca ccuuauucga      480

guugaaaauu ggaucuuuag aaaccccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaggug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaucggg acuuuguaga ggggaugucc      660

ggagguacuu ggguugaugu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gacaaaccaa cagucgauau agaacugguu accacgacug ugucaaacau ggcugaggua      780

cguaguuacu gcuaugaagc cagcauuucu gacauggcgu ccgauucacg cugucccaca      840

caaggagagg ccuaccuuga caagcagagc gacacccagu augugugcaa aagaacuuug      900

gucgauaggg gcugggguaa ugggugugga cuguucggca agggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa aaagaugacc gggaaaucca uccagccuga aaaccucgag     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaaugacacg     1080

ggccaugaga ccgaugaaaa ccgagccaag gucgagauca caccaaauuc accccgcgcu     1140

gaagccacuc uugggggauu cggcucccug gguuuggacu gugagccuag aaccgggcuc     1200

gauuuuagug accuguauua cuuaacaaug aacaauaaac acuggcuggu gcauaaggaa     1260

ugguuccacg auauuccacu cccguggcac gcaggcgccg acacuggaac accccauugg     1320

aacaauaaag aggcucuugu agaguuuaag gaugcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaagg ggccgugcau accgcuuugg ccggagcacu agaggccgaa     1440

auggacggug cuaaaggccg gcuguccucu gggcaccuca agugccgccu gaaaauggau     1500

aagcugagac ugaaaggagu cagcuauuca uuguguacug ccgcauucac guuuacaaag     1560

auaccugcgg agacccucca uggcacugug accguugaag ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgacaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gagaguaccg agaacuccaa aaugaugcug     1740

gaacuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaagaaaauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggau acagccuggg acuucggaag ugugggcggu     1920

gcgcugaaua gccuugggaa gggcauccac cagauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaacacca aaaaugguag cauauccuug augugucucg cacuuggggg aguuuuaauc     2100

uuccugucaa cggccgucag ugcugaugug ggcugcucug uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8401
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 3 opt

<400>  8401
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggugu ccgacucacg cugucccaca      840

caaggagaag cguaccuuga caaacagagc gauacccagu augucugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu aguuacaugc      960

gccaaguuug caugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu gcacggaucg caacacucug guaugaucgu caacgauacg     1080

ggccaugaga ccgacgagaa ucgagcuaaa guggaaauca caccaaacuc accccgcgcc     1140

gaggcuacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu acauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auacuggaac accccauugg     1320

aauaacaagg aagcccuugu ugaguuuaaa gacgcucacg cgaagaggca gaccgugguc     1380

gugcugggca gccaggaggg ggcaguccau accgccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauuca uuguguacug cugccuucac guuuacaaaa     1560

auaccugcag aaacccucca uggcacuguu accguggagg uacaguacgc ggguacagac     1620

gggccaugca agguccccgc ucaaauggcc guggauaugc agacccugac uccuguuggc     1680

agguuaauca cagcaaaccc agugauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgucauag gcguggggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaaag ccuuugaggc uaccguacgg     1860

ggcgccaagc gaauggcagu gcucggggac acagcuuggg auuucggaag uguuggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcggccuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cuuuaggggg aguccugauc     2100

uuccugucaa cggcagugag ugccgacgug ggcugcucug ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8402
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 3 opt

<400>  8402
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccaaug uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacguggua gcgcuuacua uauguaccug gauagaaacg acgccggcga ggcaaucucc      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucuua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacau gaccucaaca uggguggucu augguaccug ucaucacaag      360

aaaggggagg cuaggcggag ucgcagagca gugacucuac cgagccauuc cacaaggaag      420

cuccaaaccc ggucucagac guggcuggag ucgcgcgaau acacuaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggcu caagcaccag ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu ccauuaggug caucggcgug ucuaaucggg auuuuguaga agggaugagc      660

ggagguacau ggguugacgu ggucuuagag cauggcgggu gugugacugu gauggcacag      720

gauaagccaa ccgucgacau agagcugguu acaaccacgg uguccaacau ggcugaagua      780

cguucauacu gcuaugaggc caguauuagc gauauggcgu cugacucccg cugucccacu      840

caaggagaag ccuaccuuga caaacaguca gauacacagu augugugcaa gagaaccuug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcagccu agugacuugc      960

gcaaaguuug cuugcaguaa gaaaaugaca gggaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu ucacggaagc caacacucgg guaugaucgu gaaugauacc     1080

ggccaugaga cggacgagaa ccgagccaaa gucgaaauca ccccaaauuc uccccgcgcu     1140

gaggccacac uugggggauu cggcucccug gguuuggauu gugaaccuag aacugggcuc     1200

gacuuuucag aucuguauua cuuaaccaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacaggaac uccccauugg     1320

aacaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gucaggaggg ggccgugcau acagcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauagc uuguguaccg ccgcauucac uuuuacgaaa     1560

auaccugcgg aaacacucca uggcaccgug acuguugagg ugcaguacgc ugguaccgac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugccaaucc aguuauuaca gaaucaacgg agaacaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguag cggaucuacu aucgguaagg cuuuugaagc cacagugcgg     1860

ggcgcaaaac gaauggcugu acucggggac accgccuggg auuucggauc agugggcggu     1920

gcgcugaaua gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaacacua agaaugguuc gaucagcuug augugucucg cauuaggggg aguucugaua     2100

uuccugucca ccgccgucuc agcugacgug ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8403
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 3 opt

<400>  8403
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauca caccaaacuc accccgcgcu     1140

gaggccgcac uugggggauu cggcucccug gguuuggauu gugaaccuag aacugggcuc     1200

gacuuuagug aucuguauua cuuaaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcug auacaggaac uccccauugg     1320

aauaacaagg aagcgcuugu agaguuuaaa gacgcacacg ccaagaggca gaccguugug     1380

guccugggca gccaggaggg ggcugugcau acagccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguaccg cagcguucac uuuuacgaaa     1560

auaccugcug aaacacucca uggcaccgug acuguugagg ugcaguacgc cgguaccgac     1620

gggccaugca agguacccgc acaaauggcc gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugcuaaccc aguuauuaca gaaaguacgg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaaag ccuuugaggc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggaag ugugggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaaca cugaucggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauuuccuug augugucucg ccuuaggggg aguucugaua     2100

uuccugucaa cggcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8404
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 3 opt

<400>  8404
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gaaaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggagguuacg      120

cgacgugguu ccgcuuacua uauguaccug gacagaaaug augccggcga agcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggacuu aggacacaug      240

ugugaugcca cuaugucaua cgagugcccu augcuggacg aaggcgugga gccagaugac      300

guagauuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggaa ucacgcgagu acaccaagca ccuuauucga      480

guugaaaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

uuagccuacu cuauuaggug caucggcgug agcaaccggg acuuuguaga ggggaugucc      660

ggagguacuu ggguugaugu gguccuggag cauggcgggu gugugaccgu gauggcacag      720

gacaagccua cagucgauau agaacuuguu accacgacug ugucaaauau ggcugaggua      780

cguaguuacu gcuaugaagc cagcauuucu gacauggcgu ccgauucacg cuguccaaca      840

caaggagagg ccuacuugga caaacagagc gacacccagu augugugcaa gagaacucug      900

gucgauaggg gcugggguaa cgggugugga cuauucggca aaggcagucu cgugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccagcccga aaaucuggag     1020

uaccggauua ugcuuagcgu ucacggaucg caacacucug guaugaucgu gaacgacacg     1080

ggccaugaga ccgaugaaaa ucgagccaaa gucgagauca caccuaacuc cccacgcgcu     1140

gaagccacuc ugggaggguu cggcucauug ggucucgacu gugagcccag aaccgggcug     1200

gauuuuagug acuuauauua ccugacaaug aauaacaagc acuggcucgu gcauaaagaa     1260

ugguuccacg auauuccucu uccauggcac gcaggcgccg acacuggaac cccgcauugg     1320

aauaacaagg aggcucuggu agaguuuaaa gaugcgcacg caaagaggca gacaguugug     1380

gucuugggca gccaggaagg ggccgugcau accgcucuag ccggagcacu ggaggccgaa     1440

auggacggug cuaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggau     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug ccgcauucac guuuacaaaa     1560

auacccgcgg agacccugca uggcacugug accguugaag ugcaguacgc ugguacagac     1620

gggccuugca agguaccagc ccaaauggca gucgacaugc agaccuuaac ucccgugggc     1680

aggcugauca cagccaaccc uguuauuacg gagaguaccg aaaauuccaa gaugaugcua     1740

gagcucgauc cacccuucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaaac gaauggcugu acugggggau acagccuggg acuucggaag ugugggcggu     1920

gcgcuuaaca gcuuggggaa aggcauccac cagauuuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uucgcaaauc cucauuggaa cucugcuuau guggcugggc     2040

uugaauacca agaacgguag cauaucccuc augugucugg cauuaggggg aguucugauc     2100

uuccuaucaa cggccgucag ugcugaugug ggcugcucug uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8405
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 3 opt

<400>  8405
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcaua agcaaccggg auuuugugga agggaugucc      660

ggagguacuu ggguagacgu uguguuagag cauggcgggu gugucaccgu gauggcacag      720

gauaagccaa caguggacau ugagcugguc accacgacug uuucaaauau ggcugaagug      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu gcacggaucg caacacucug guaugaucgu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa guggaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcauacug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuuccg aucuguauua cuuaacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acaucccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu ggaguuuaaa gacgcgcacg caaagaggca gaccguaguu     1380

gugcugggca gucaggaggg ggccguccau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gucuuauagc uuguguacug ccgcauucac guuuacaaaa     1560

auuccugcgg aaacccucca uggcacuguc accguggagg uucaguacgc ugguacagac     1620

gggccaugca aggugcccgc ccaaauggca guagauaugc agacccugac uccugucggc     1680

agguuaauca cagccaaccc agugauaacg gaaucaaccg agaauaguaa gaugaugcug     1740

gagcuagauc ccccguucgg agacuccuac auuguuaucg gcguggggga gaaaaagauc     1800

acucaccacu ggcaucguag cggaucuaca auugguaagg cuuuugaagc caccguccgg     1860

ggcgcaaaac gaauggcugu gcucggggac acagccuggg auuucggauc aguaggcggu     1920

gcgcugaaca gucuugggaa aggcauccac cagauuuucg gagccgcuuu uaagagcuug     1980

uucggcggga uguccugguu uucucaaaua cugaucggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguuc gauuagcuug augugucucg cauuaggggg agugcugauc     2100

uuccugucca cggccguuuc agcugacguc ggcugcagug uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8406
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 3 opt

<400>  8406
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacacu      240

ugugacgcca ccaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac aaccaguacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacccuac cgucccauuc uauuaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauccga      480

guugagaacu ggauauuuag aaaucccgga uucgcccugg cugccgcggc aaucgccugg      540

uugcucggca gcaguacauc ccagaaagug auuuaucugg ucaugauccu gcucauugcu      600

ccugccuacu cuaucaggug cauaggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau ugagcugguu accacgacug ugucaaauau ggcugaagua      780

cguaguuacu gcuaugaggc cagcaucucu gauauggcgu ccgacucacg cugucccaca      840

caaggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uucagccuga gaaucucgaa     1020

uaccggauca ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgagccaaa gucgaaauua caccaaacuc accccgcgcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauaccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

aucccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauua cagccaaccc aguuaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac auagugauug gcgucgggga gaaaaagauc     1800

acucaccacu ggcaucguuc uggaucaaca aucgguaagg cuuuugaggc caccgugcgg     1860

ggcgcaaaac gaauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauucac cagaucuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauu cugauaggaa cucuccugau guggcuuggc     2040

cugaaugcaa agaacgguag caucuccuug augugucucg ccuuaggggg aguucugauu     2100

uuccugucaa ccgcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8407
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 3 opt

<400>  8407
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cucaaaggag ccggcgcgca gugacacuac cgucccauuc uaccagaaag      420

cuccagacga ggucgcagac uuggcuggag ucacgggaau acaccaagca ccuuauucgc      480

guugagaacu ggaucuuucg aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuagaug caucggcgug agcaacaggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacaa      720

gauaagccaa cagucgacau agagcugguu accacgacug ugucaaauau ggcugaagua      780

cggaguuacu gcuaugaggc cagcauuucu gauauggcgu ccgacucacg uugucccaca      840

cagggagaag ccuaccuuga caaacagagc gauacccagu augugugcaa gcgcacuuug      900

gucgacagag gcugggguaa cgggugugga cuguucggca aaggcagucu agugacaugc      960

gcaaaguuug cuugcucuaa gaaaaugacc gggaagucca uccaaccuga gaaucucgaa     1020

uacaggauua ugcugagcgu ucacggaucg cagcacucug guaugaucgu gaacgauacg     1080

ggccaugaga ccgacgagaa ucgggccaaa gucgaaauca caccaaacuc accccgagcu     1140

gaggccacuc uugggggauu cggcucccug gguuuggauu gugaaccucg caccgggcuc     1200

gacuuuagug aucuguauua cuuaacaaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aauaacaagg aagcucuugu agaguuuaaa gacgcgcacg caaagagaca gaccguugug     1380

guccugggca gccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggcag gcuguccucu gggcaccuca aaugccggcu gaagauggac     1500

aaacuucgcc ugaagggagu cagcuauuca uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaauacgc ugguacagac     1620

gggccaugca agguacccgc ccagauggca gucgauaugc agacccugac uccugugggc     1680

agauuaauca cagccaaccc aguuauuacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcauagguc uggaucaaca aucgguaagg cuuuugaggc caccgugcgu     1860

ggcgcaaaac ggauggcugu acucggggac acagccuggg auuucggaag ugugggcggu     1920

gcgcugaaca gccuugggaa aggcauccac caaauuuucg gagccgcuuu uaaguccuug     1980

uucggcggga ugucuugguu uucgcagauc cugauuggaa cucuccugau guggcuuggc     2040

cugaauacca agaacgguag cauauccuug augugucucg cauuaggggg aguucugauc     2100

uuccugucaa cggccgucag ugcugacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8408
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 3 opt

<400>  8408
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaagccc ggucgcagac guggcuggag ucacgcgaau acacuaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcucugg ccgcggcagc cauagcuugg      540

uugcucggca gcaguaccuc ccagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcauacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacau ggguugacgu ggucuuagag cauggcgggu gugugacugu gauggcucag      720

gauaagccaa ccgucgacau agagcugguu acaaccacgg ugucaaauau ggccgaagua      780

cguaguuacu gcuaugaggc gagcauuucu gauauggccu ccgacucacg cugucccacu      840

caaggagaag cauaccuuga caaacagagc gauacacagu augugugcaa gagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agugacuugc      960

gcuaaguuug ccugcucuaa gaaaaugaca gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu ucacggaucg caacacucug guaugaucgu gaacgauacc     1080

ggccaugaga cggacgagaa ucgagcuaaa gucgaaauca ccccaaacuc accccgcgcc     1140

gaggcaacac uugggggauu cggcucccug gguuuggauu gugaaccuag aacugggcuc     1200

gacuuuagug aucuguauua cuuaaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcug auacaggaac uccccauugg     1320

aauaacaagg aagcgcuugu agaguuuaaa gacgcacacg ccaagaggca gaccguugug     1380

guccugggca gccaggaggg ggcugugcau acagccuugg caggagcccu agaagcugag     1440

auggauggug ccaagggccg gcuguccucu gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauuca uuguguaccg cagcguucac uuuuacgaaa     1560

auaccugcug aaacacucca uggcaccgug acuguugagg ugcaguacgc cgguaccgac     1620

gggccaugca agguacccgc acaaauggcc gucgauaugc agacacugac cccugugggc     1680

agguuaauca cugcuaaccc aguuauuaca gaaaguacgg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg agacagcuac aucgugauag gcgucgggga gaaaaagauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaaag ccuuugaggc aacagugcgg     1860

ggcgcuaagc gaauggccgu acucggggac accgcguggg auuucggaag ugugggcggu     1920

gcccugaaca gccuugggaa aggcauccac cagauuuucg gagcugcauu uaaguccuug     1980

uucggcggga ugucuugguu uucgcaaauc cugauuggaa cacuccugau guggcuuggc     2040

cugaauacua agaacgguag cauauccuug augugucucg ccuuaggggg aguucugauc     2100

uuccugucaa ccgcugucag ugccgacgug ggcugcucug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8409
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 3 opt

<400>  8409
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaucug acgccggcga ggcaaucuca      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcgguc ccgcagagca gugacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgaau acaccaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca guuccacauc ucagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgu gauggcacag      720

gauaagccaa cagucgacau agagcugguu accacgacug ugaguaacau ggcugaagua      780

cguagcuacu gcuaugaggc cucuauuucc gauauggcgu cagacagccg cugucccaca      840

caaggagaag ccuaccuuga caaacagagu gauacccagu augugugcaa gagaacuuug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugacaugc      960

gcaaaguuug cuugcuccaa gaaaaugacc gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagccaaa gucgaaauca caccaaauuc accccgcgcu     1140

gaggccacuc uugggggauu cggcagucug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagcg aucuguauua cuuaacaaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcaggcgccg auacuggaac accccauugg     1320

aacaauaagg aagcucuugu agaguuuaaa gacgcgcacg caaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggccgugcau accgcuuugg ccggagcacu agaagccgag     1440

auggauggug cuaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacug ccgcauucac guuuacaaaa     1560

auaccugcgg aaacccucca uggcacugug accguugagg ugcaguacgc ugguacagac     1620

gggccaugca agguacccgc ccaaauggca gucgauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc aguuauuacg gaauccaccg agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcgucgggga gaaaaagauu     1800

acucaccacu ggcaucguuc aggaaguaca aucgguaagg cuuuugaagc caccgugcgg     1860

ggcgcaaagc gaauggcugu acucggggac acagccuggg auuucggaag cgugggcggu     1920

gcgcuuaauu cccuggggaa aggcauccac cagauuuucg gagccgcuuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaacacca agaaugguuc cauaucauug augugucucg cauuaggggg aguucugauc     2100

uuccugagua cggccgucuc ugcugacgug ggcugcagcg uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8410
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 3 opt

<400>  8410
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagc cgaaguuacc      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa ccaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac aaccagcacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacuuc ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugaguaacau ggcagaagua      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccacc      840

caaggagaag cguaccuuga caaacagagu gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugaccugc      960

gccaaguuug caugcuccaa gaaaaugacg gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacc     1080

ggccaugaga cagacgagaa ccgagcuaaa gucgaaauca cuccaaauuc accccgcgcc     1140

gaggcuaccc uugggggauu cggcagucug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac accggcgccg auacaggaac uccccauugg     1320

aacaauaagg aagcacuugu agaguuuaaa gacgcccacg cuaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggcggugcau acggcauugg ccggagcucu agaagccgag     1440

auggauggug caaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacag ccgcuuucac cuuuacuaaa     1560

auaccugccg aaacccucca uggcacagug accguugagg ugcaguacgc agguacugac     1620

gggccaugca agguacccgc gcaaauggcu gucgauaugc agacacugac gccugugggc     1680

agguuaauca ccgccaaccc aguuauuacu gaaggaacag agaacuccaa gaugaugcug     1740

gagcuagauc ccccguucgg cgacagcuac aucgugauag gggucggaga aaagaagauu     1800

acccaccacu ggcaucguuc ugguucaaca aucggcaaag cauuugaggc cacugugcga     1860

ggggcuaagc ggauggccgu acucggagac accgcauggg auuucggcag uguggguggg     1920

guucugaaua gccuuggcaa aggaauccac cagauuuucg gcgcugccuu uaaguccuug     1980

uucgggggaa ugucuugguu uucgcaaauc cugauuggca cgcuccugau guggcuuggu     2040

cugaacacca agaaugggag cauauccuug augugucucg cgcugggagg cgucuuaauc     2100

uuccugucaa cagccgugag ugcugacgug gguugcucug ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8411
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 3 opt

<400>  8411
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgc ggaaguuacg      120

cgacgugguu ccgcuuacua uauguaccug gauagaaaug acgccggcga ggcaaucucu      180

uuccccacca cauuggggau gaacaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca cuaugucaua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaauac cacaaguacc uggguggucu augguacuug ucaucacaag      360

aaaggggagg cuaggcggag ccgcagagca gugacacuac cgucccauuc uaccaggaag      420

cuccaaacgc ggucgcagac uuggcuggag ucacgcgaau acaccaagca ccuuauucga      480

guugagaacu ggaucuuuag aaaucccgga uucgcccugg cugccgcggc aauagccugg      540

uugcucggca gcaguacauc ccagaaagug aucuaucugg ucaugauucu gcucaucgcu      600

ccugccuacu cuauuaggug caucggcgug agcaaccggg auuuuguaga agggaugucc      660

ggagguacuu ggguugacgu ggucuuagag cauggcgggu gugugaccgc aauggcucag      720

gauaagccaa caguggacau agagcugguc accacgacug uuucaaauau ggccgaagug      780

cguaguuacu gcuaugaggc gagcauuucu gauauggccu ccgacucacg cugucccaca      840

caaggagaag cauaccuuga caaacagagc gauacccagu auguaugcaa gagaacuuug      900

guggacaggg gcugggguaa cgggugugga cuguucggca aaggcagucu agucacaugc      960

gcuaaguuug ccugcucuaa gaaaaugacc gggaagucca uccagccuga gaaucucgaa     1020

uaccggauua ugcugagcgu gcacggaucg caacacucug guaugcuugu uaacgauacg     1080

ggccaugaga ccgacgagaa ucgagcuaaa guggaaauca caccaaacuc accccgcgcc     1140

gaggcaacuc ugggaggguu cggcuccuug ggucucgauu gugaaccuag aaccgggcug     1200

gacuuuagug auuuauauua ccugacaaug aauaacaagc acuggcucgc ccauaaagag     1260

ugguuccacg acaucccacu uccguggcac gcuggcgcgg caacuggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gaugcucacg ccaagaggca gaccguggua     1380

guuuugggca gccaggaggg ggcagugcau accgcccuag cuggagcccu ggaagcagag     1440

auggacggug cgaagggccg gcucuccucu gggcaccuga aaugccgccu uaagauggau     1500

aaacugagau ugaagggagu cagcuauuca cucuguacug cugccuucac guuuacaaaa     1560

auuccugcag aaaccgugga cggcacuguc accguggagg gucaguacgg gggcacagac     1620

ggaccaugca agguucccgc ccaaauggcu guggauaugc agacccugac uccuguaggc     1680

agguuaauaa cagccaaccc agucaucacg gaaaguaccg aaaauuccaa gaugaugcug     1740

gagcuagauc ccccguucgg ggacagcuac auugugaucg gaguugguga gaaaaagaua     1800

acucaucacu ggcaccguuc uggcucaaca auugggaaag cauuugaggc uaccgugcgg     1860

ggagccaagc gaauggcggu ccucggcgac acagccuggg auuucgguag uguagggggc     1920

gcucugaaca gccuuggaaa aggcauccau cagaucauug gggcagccuu caaguccuug     1980

uuuggaggca ugucuugguu cucgcaaauc cugauuggua cucuccugau guggcuuggg     2040

cugaauacca agaacggaag cauauccuug augugucucg cucugggcgg uguguuaauc     2100

uuucugucaa cggccgugag uggcgggguu ggaugcucug ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8412
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 3 opt

<400>  8412
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagc cgaaguuacc      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa ccaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac aaccagcacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacuuc ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugaguaacau ggcagaagua      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccacc      840

caaggagaag cguaccuuga caaacagagu gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugaccugc      960

gccaaguuug caugcuccaa gaaaaugacg gggaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacc     1080

ggccaugaga cagacgagaa ccgagcuaaa gucgaaauca cuccaaauuc accccgcgcc     1140

gaggcuaccc uugggggauu cggcagucug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auaccggaac accccauugg     1320

aacaauaagg aagcccuugu agaguuuaaa gacgcucacg cgaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggcagugcau acugccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacgg cugccuucac auuuuccaaa     1560

auaccugcag aaacccucca uggcacugug accgaggaag uucaguacgc ggguacagac     1620

gggccaugca aggugcccgc ucaaauggcc guagauaugc agacccugac uccugucggc     1680

agguuaauca cagcaaaccc agugauuacg gagagcaccg agaacucuaa gaugaugcug     1740

gaacuagauc ccccguucgg agacucauac aucguuauag gcguggggga gaaaaagauu     1800

acucaccacu ggcaucguag uggaagcaca aucgguaaag ccuuugaagc uaccguccgg     1860

ggcgccaagc gaauggcagu gcucggggac acagcuuggg auuucggauc cguaggcggu     1920

gcccugaaua gccuugggaa aggcauccac cagauuuucg gagcggccuu uaagucguug     1980

uucggcggga ugagcugguu uucccaaauc cugauuggaa cucuccugau guggcuuggc     2040

cugaacacca agaaugguuc aauaaguuug augugucucg cuuuaggggg agugcugauc     2100

uuccugucua cggcaguuag cgccgacguc ggcugcuccg uggauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8413
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 3 opt

<400>  8413
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagc cgaaguuacc      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa ccaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac aaccagcacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacuuc ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugaguaacau ggcagaagua      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccacc      840

caaggagaag cguaccuuga caaacagagu gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugaccugc      960

gccaaguuug caugcuccaa gaaaaugacg gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacc     1080

ggccaugaga cagacgagaa ccgagcuaaa gucgaaauca cuccaaauuc accccgcgcc     1140

gaggcuaccc uugggggauu cggcagucug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auaccggaac accccauugg     1320

aacaauaagg aagcccuugu agaguuuaaa gacgcucacg cgaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggcagugcau acugccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacgg cugccuucac auuuaccaaa     1560

auaccugcag aaacucucca uggcaccgug acaguugagg ugcaguacgc ggguaccgac     1620

gggccaugca agguacccgc ucaaauggcc gucgauaugc agacucugac accugugggc     1680

agguuaauca cggcaaaccc aguuauuacc gaauccacug agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcgucgggga aaagaagauu     1800

acacaccacu ggcaucguuc aggaaguacc aucgguaaag ccuuugaggc uacagugcgg     1860

ggcgccaagc gaauggcagu acucggggac acugcuuggg auuucggaag cgugggcggu     1920

gcccuuaauu cccuggggaa aggcauccac cagauuuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaacacga agaaugguuc cauaucauug augugucucg cuuuaggggg aguucugauc     2100

uuccugagua ccgcagucuc ugccgacgug ggcugcagcg uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8414
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 3 opt

<400>  8414
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagu ugaagugacc      120

cgacguggua augccuacua uauguaccug gauagauccg acgcgggcga ggcuaucucu      180

uuccccacaa cuauggggau gaacaaaugc uauauucaga ucauggauuu gggacacaug      240

ugugacgcca ccaugucaua cgaaugcccu auguuagaug agggcguaga accagacgau      300

guggacuguu ggugcaauac aaccaguacu ugggucgugu augguacaug ucaucacaag      360

aaaggggagg caaggcggag ccgcagagcc guuacccugc cgucccauuc uacgaggaag      420

cuacaaacuc ggucgcagac cuggcucgag ucacgcgaau acacaaagca ccugauucga      480

guggagaacu ggaucuuuag aaaucccgga uucgcucuug cagccgcugc cauagcgugg      540

cuguugggca gcaguacuuc ccagaaaguc aucuaucucg ugaugauucu gcugaucgca      600

cccgccuacu cuauuaggug caucggcgua agcaaccggg auuuuguuga agggaugucc      660

ggagguaccu ggguggacgu cgugcucgag cauggcgggu gugugacagu cauggcucag      720

gauaagccaa ccguugacau agaguuagug acgacuacag uaucaaauau ggccgaagug      780

cguaguuacu gcuaugaggc aagcauuucu gauauggcuu ccgacucacg cugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacucagu augucugcaa gagaacacuu      900

guggacaggg gcugggguaa cgggugugga uuguucggca aaggcagucu gguuaccugc      960

gcgaaguuug ccugcucuaa gaaaaugacg gggaagucca uccagccuga gaaucuagaa     1020

uaccggauua ugcucagcgu gcacggaucg caacacucug guaugaucgu caacgauacc     1080

ggccaugaga cagacgagaa ucgagcaaaa guggaaauca cuccaaacuc cccucgcgcu     1140

gaggccaccc uugggggauu cggcucacug ggucuggauu gugaaccuag aacaggguug     1200

gacuuuagug aucucuauua ccugacuaug aauaacaagc acugguuagu acauaaagag     1260

ugguuccacg acauuccacu gccguggcac gcuggcgccg auaccggaac accccauugg     1320

aauaacaagg aagcacucgu ugaguuuaaa gacgcccacg cuaagaggca gaccgugguc     1380

gugcuuggca gccaggaggg ggcgguccau acugcacugg ccggagcuuu ggaagccgag     1440

auggauggug caaagggccg gcuauccucu gggcaccuga aaugccgccu caagauggac     1500

aaacugagac uuaagggagu gagcuauuca cuguguacgg ccgcuuucac auuuaccaaa     1560

auaccugccg aaacuuugca uggcaccguu acaguggagg uacaguacgc agguaccgac     1620

gggccaugca agguccccgc gcaaauggcu guggauaugc agacucucac accuguuggc     1680

aggcugauca cggccaaccc agugauuacc gaaaguacug aaaauuccaa gaugauguua     1740

gagcuggauc ccccguucgg agacagcuac aucgucauag gcguggggga gaaaaagauu     1800

acacaccacu ggcaucguuc uggaucaacc aucgguaaag cauuugaggc cacaguacgg     1860

ggcgcuaagc gaauggccgu gcuaggggac acugcauggg auuucggaag uguuggcggu     1920

gcucucaaca gccuggggaa aggcauccac cagauuuucg gagccgcguu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauc uugauuggaa cccugcucgu cuggcugggc     2040

cuuaauacga agaacgguag cauaucccug auguguuugg cccucggggg agugcugauc     2100

uucuuaucaa ccgcugugag ugcagacguc ggcugcucug uugauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8415
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 3 opt

<400>  8415
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagc cgaaguuacc      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa ccaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

guagacuguu ggugcaacac aaccagcacu uggguggucu augguacaug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guugagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacuuc ucagaaagug aucuaucugg ucaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcgug uccaaucggg auuuuguaga agggauguca      660

ggagguaccu ggguugacgu ggucuuagag cauggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau agagcugguu acgacuacag ugaguaacau ggcagaagua      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccacc      840

caaggagaag cguaccuuga caaacagagu gauacucagu augugugcaa gagaacauug      900

gucgacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugaccugc      960

gccaaguuug caugcuccaa gaaaaugacg gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ucacggaucu caacacuccg guaugaucgu gaaugauacc     1080

ggccaugaga cagacgagaa ccgagcuaaa gucgaaauca cuccaaauuc accccgcgcc     1140

gaggcuaccc uugggggauu cggcagucug gguuuggauu gugaaccuag aacagggcuc     1200

gacuuuagcg aucuguauua cuuaacuaug aacaauaagc acuggcuggu gcauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auaccggaac accccauugg     1320

aacaauaagg aagcccuugu agaguuuaaa gacgcucacg cgaagaggca gaccguugug     1380

guccugggcu cccaggaggg ggcagugcau acugccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu cucauauagu uuguguacgg cugccuucac auuuaccaaa     1560

auaccugcag aaacucucca uggcaccgug acaguugagg ugcaguacgc ggguaccgac     1620

gggccaugca agguacccgc ucaaauggcc gucgauaugc agacucugac accugugggc     1680

agguuaauca cggcaaaccc aguuauuacc gaauccacug agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcgucgggga aaagaagauu     1800

acacaccacu ggcaucguuc aggaaguacc aucgguaaag ccuuugaggc uacagugcgg     1860

ggcgccaagc gaauggcagu acucggggac acugcuuggg auuucggaag cgugggcggu     1920

gcccuuaauu cccuggggaa aggcauccac cagauuuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cccuccugau guggcuuggc     2040

cugaacacga agaaugguuc cauaucauug guuugucucg cuuuaggggg aguccugauc     2100

uuccugagua ccgcaguguc ugccgacgug ggcugcagcg ucgauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8416
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 3 opt

<400>  8416
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcacc       60

gacacaagcg uggggauagu cggacugcuc cuuacuacgg cuauggcagu ugaagugacc      120

cgacgugguu ccgccuacua uauguaccug gauagaucug acgcgggcga ggcuaucuca      180

uuccccacaa cuuuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcca ccaugaguua cgaaugcccu augcuggaug agggcguaga accagacgau      300

guggacuguu ggugcaacac aaccagcacu ugggucgugu augguacaug ucaucacaag      360

aaaggggagg caaggcgguc ccgcagagcc guuacccuac cgucucauuc gacgaggaag      420

cuccaaacuc ggucacagac cuggcuggag agccgcgaau acacaaagca ccuuauucga      480

guggagaauu ggaucuuuag aaaccccgga uucgcucugg cagccgcugc cauagcgugg      540

uugcucggca guuccacuuc ucagaaaguc aucuaucugg ugaugauucu gcucaucgcc      600

ccugcauaca gcauuaggug caucggcgua uccaaucggg auuuuguuga agggauguca      660

ggagguaccu ggguggacgu cguguuagag cauggcgggu gugugacagu cauggcucag      720

gauaagccag ccguugacau agagcuggug accacgacug uaaguaacau ggcagaagug      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccaca      840

caaggagaag cguaccuuga caaacagagu gauacccagu augucugcaa gagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu aguuacaugc      960

gccaaguuug caugcuccaa gaaaaugacc gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu gcacggaucu caacacuccg guaugaucgu caaugauacg     1080

ggccaugaga ccgacgagaa ccgagcuaaa guggaaauca caccaaauuc accccgcgcc     1140

gaggcuacuc uugggggauu cggcagucug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagcg aucuguauua cuuaacaaug aacaauaagc acuggcuggu acauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auacuggaac accccauugg     1320

aacaauaagg aagcccuugu ugaguuuaaa gacgcucacg cgaagaggca gaccgugguc     1380

gugcugggcu cccaggaggg ggcaguccau accgccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu gucauauagu uuguguacug cugccuucac guuuacaaaa     1560

auaccugcag aaacccucca uggcacuguu accguggagg uacaguacgc ggguacagac     1620

gggccaugca agguccccgc ucaaauggcc guggauaugc agacccugac uccuguuggc     1680

agguuaauca cagcaaaccc agugauuacg gaauccaccg agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgucauag gcguggggga aaagaagauu     1800

acucaccacu ggcaucguuc aggaaguaca aucgguaaag ccuuugaggc uaccguacgg     1860

ggcgccaagc gaauggcagu gcucggggac acagcuuggg auuucggaag cguuggcggu     1920

gcccuuaauu cccuggggaa aggcauccac cagauuuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cucuccuggu cuggcuuggc     2040

cugaacacca agaaugguuc cauaucauug acgugucucg cuuuaggggg agugcugauc     2100

uuccugagua ccgcaguguc ugccgacguc ggcugcagcg uugauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8417
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 3 opt

<400>  8417
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgu ugaagugacg      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacca cauuggggau gaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa cuaugaguua cgaaugcccu augcuggaug agggcguaga accagacgau      300

guggacuguu ggugcaacac cacaagcacc ugggucgugu augguacuug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu guuacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgaau acaccaagca ccuuauucga      480

guggagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgu cauagcgugg      540

uugcucggca guuccacauc ucagaaagug aucuaucugg uaaugauucu gcucaucgcc      600

ccugcauaca gcauuaggug caucggcguu uccaaucggg auuuugugga agggauguca      660

ggagguacuu gggucgacgu gguguuagag cauggcgggu gugucaccgu uauggcucag      720

gauaagccaa caguggacau agagcuggua accacgacug ugaguaacau ggccgaaguc      780

cguagcuacu gcuaugaggc aucuauuucc gauauggcuu cagacagccg cugucccaca      840

caaggagaag ccuaccuuga caaacagagu gauacccagu augugugcaa gagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugacaugc      960

gcgaaguuug ccugcuccaa gaaaaugacc gggaaaagca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ccacggaucu caacacuccg guaugaucgu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagcaaaa guagaaauca caccaaauuc accccgcgcu     1140

gaggccacuc uugggggauu cggcagucug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagcg aucuguauua cuuaacaaug aacaauaagc acuggcuggu ucauaaagag     1260

ugguuccacg acauuccacu cccguggcac gcuggcgccg auacuggaac accccauugg     1320

aacaauaagg aagcacuugu ggaguuuaaa gacgcccacg cuaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcggugcau accgcauugg ccggagcucu agaagccgag     1440

auggauggug caaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu uucauauagu uuguguacug ccgcuuucac guuuacaaaa     1560

auaccugccg aaacccucca uggcacugug accguagagg uccaguacgc agguacagac     1620

gggccaugca aggugcccgc gcaaauggcu guugauaugc agacccugac uccugugggc     1680

agguuaauca cagccaaccc agucauuacg gaauccaccg agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcguagggga aaagaagauu     1800

acucaccacu ggcaucguuc aggaaguaca aucgguaaag cauuugaggc caccgugcgg     1860

ggcgcuaagc gaauggccgu ucucggggac acagcauggg auuucggaag cgucggcggu     1920

gcucuuaauu cccuggggaa aggcauccac cagauuuucg gagccgcguu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cucuccuggu guggcuuggc     2040

cugaacacca agaaugguuc cauaucauug acgugucucg ccuuaggggg agugcugauc     2100

uuccugagua ccgcugucuc ugcagacguu ggcugcagcg uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8418
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 3 opt

<400>  8418
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgu ugaagugacg      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcga ggccaucuca      180

uuccccacca cauugggggu aaauaaaugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa cuaugaguua cgaaugcccu augcuggaug agggcgugga accagacgau      300

gucgacuguu ggugcaacac cacaagcacc ugggugguuu augguacuug ucaucacaag      360

aaaggggagg ccaggcgguc ccgcagagcu gugacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgaau acaccaagca ccuuauucga      480

gucgagaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacauc ucagaaagug aucuaucugg uaaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcguu uccaaucggg auuuugugga agggauguca      660

ggagguacuu gggucgacgu gguguuagag cauggcgggu gugucaccgu uauggcccag      720

gauaagccaa caguggacau agagcuggua accacgacug ugaguaacau ggcagaaguc      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccaca      840

caaggagaag cguaccuuga caaacagagu gauacccagu augugugcaa gagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca aaggcucucu agugacaugc      960

gccaaguuug caugcuccaa gaaaaugacc gggaagucca uccagccuga gaaccucgaa     1020

uaccggauua ugcugucggu ccacggaucu caacacuccg guaugaucgu gaaugauacg     1080

ggccaugaga ccgacgagaa ccgagcuaaa guagaaauca caccaaauuc accccgcgcc     1140

gaggcuacuc uugggggauu cggcagucug gguuuggauu gugaaccuag aaccgggcuc     1200

gacuuuagcg aucuguauua cuuaacaaug aacaauaagc acuggcuggu ucauaaagag     1260

ugguuccacg acauuccacu cccguggcac gccggcgcag auacuggaac accccauugg     1320

aacaauaagg aagcccuugu ggaguuuaaa gacgcucacg cgaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcagugcau accgccuugg cuggagcccu agaagcagag     1440

auggauggug ccaagggccg gcugucuagc gggcaccuca aaugccgccu gaagauggac     1500

aaacuuagac ugaagggagu uucauauagu uuguguacug cugccuucac guuuacaaaa     1560

auaccugcag aaacccucca uggcacugug accguagagg uccaguacgc ggguacagac     1620

gggccaugca aggugcccgc ucaaauggcc guugauaugc agacccugac uccugugggc     1680

agguuaauca cagcaaaccc agucauuacg gaauccaccg agaacagcaa gaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac aucgugauag gcguagggga caaaaagauu     1800

acucaccacu gguaucguuc aggaaguaca aucgguaaag ccuuugaagc uaccgugcgg     1860

ggcgccaagc gaauggcagu ucucggggau acagcuuggg acuucggaag cgucggcggu     1920

gcccugaaua gccuugggaa aggcauccau cagauuuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauc cugauuggaa cucuccuggu guggcuuggc     2040

cugaacacca agaaugguuc cauaucauug acgugucucg cuuuaggggg agugcugauc     2100

uuccugagua ccgcagucuc ugccgauguu ggcugcagcg uggacuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8419
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 3 opt

<400>  8419
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agaaaaagcg gcgcggcgcu       60

gacaccagcg uggggauagu cggacugcuc cuuacaacug caauggccgu ugaagugacg      120

cgacgugguu ccgcguacua uauguaccug gauagaucug acgcuggcaa agccaucuca      180

uuccccacca cauugggggu aaauaagugc uauauucaga ucauggauuu aggacacaug      240

ugugacgcaa cuaugaguua cgagugcccu augcuggaug aaggcgugga gccagacgau      300

gucgacuguu ggugcaacac cacaagcacc ugggugguuu augguacuug ucaucacaaa      360

aagggggaag ccaggcgguc ccgcagagcu gugacacuac cgucucauuc gaccaggaag      420

cuccaaacgc ggucacagac uuggcuggag agccgcgagu acaccaaaca ccuuauucga      480

gucgaaaauu ggaucuuuag aaaccccgga uucgcacugg ccgcugccgc gauagcaugg      540

uugcucggca guuccacauc ucagaaggug aucuaucugg uaaugauucu gcucaucgcc      600

ccugcuuaca gcauuaggug caucggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguacuu gggucgacgu gguguuagaa cauggcgggu gugucaccgu uauggcccag      720

gauaaaccaa caguggacau agagcuggua accacgacug ugaguaacau ggcagagguc      780

cguagcuacu gcuaugaggc uucuauuucc gauauggccu cagacagccg cugucccaca      840

caaggagaag cguaccuuga caagcagagu gauacccagu augugugcaa aagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugacaugc      960

gccaaguuug caugcuccaa aaagaugacc gggaaaagca uccagccuga aaaccucgag     1020

uaccggauua ugcugucggu ccacggaucu caacacuccg guaugaucgu gaaugauauc     1080

ggccaugaaa cggacgagaa ccgagcuaag guagagauua ccccaaauuc accccgcgcc     1140

gaagcuacac uugggggauu cggcagucug gguuuggauu gugagccuag aacugggcuc     1200

gacuuuagcg aucuguauua cuuaaccaug aacaauaaac acuggcuggu ucauaaggaa     1260

ugguuccacg acauaccacu cccguggcac gccggcgcag auacaggaac uccccauugg     1320

aacaauaaag aggcccuugu ggaauuuaag gacgcucacg cgaagaggca gaccgucgug     1380

guccugggcu cccaggaggg ggcagugcau acagccuugg cuggagcccu agaggcagaa     1440

auggauggug ccaaaggccg gcugucuagc gggcaccuca agugccgccu gaaaauggac     1500

aagcugagac ugaaaggagu uucauauagu uuguguaccg cugccuucac uuuuacgaag     1560

aucccugcag agacacucca uggcaccgug acuguagaag uccaguacgc ggguaccgac     1620

gggccaugca aggugcccgc ucaaauggcc guugauaugc agacacugac cccugugggc     1680

agguuaauua cugcaaaccc agucaucaca gaguccacgg aaaacagcaa aaugaugcug     1740

gagcuagauc ccccguucgg agacucuuac auagugauug gcguagggga caagaaaauc     1800

acccaccacu ggcaucguuc aggaaguacu aucgguaagg ccuuugaggc uacagugcgg     1860

ggcgccaaac gaauggcagu ucucggggau accgcuuggg acuucggaag cgucggcggu     1920

gcccuuaauu cccuugggaa gggcauucac cagaucuucg gagcggccuu uaagucuuug     1980

uucggcggga ugucgugguu uagccaaauu cugauaggaa cacuccuggu guggcuuggc     2040

cugaacacua aaaaugguuc caucucauug accugucucg cuuuaggggg agugcugauu     2100

uuccugagua cggcagucuc ugccgauguu ggcugcagcg uggacuuuuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8420
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 3 opt

<400>  8420
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucacacugg uggcugucgc caucgcgugg      540

uugcucggca guuccacuuc ucagaaggug auuuaccugg uaaugauccu gcucauugca      600

cccgccuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguaccu gggucgacgu gguguuagaa cacggcgggu gugucacagu uauggcucag      720

gauaaaccua ccguggacau ugagcuggua acgacuacag ugaguaacau ggccgagguc      780

cguagcuacu gcuacgaagc aucuaucucc gauauggcuu cagacagccg cuguccaacc      840

caaggagagg ccuaucuuga caagcagagu gauacucagu acgugugcaa aagaacauug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugaccugc      960

gcgaaguuua cgugcuccaa aaagaugacc gggaaaagca uucagcccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu caacauuccg guaugaucgu gaaugauaca     1080

ggccacgaaa cugacgagaa ccgagccaag guagagguua ccccuaauuc accacgcgca     1140

gaagcuacac uugggggauu cggcagucug gguuuggauu gugagcccag aacugggcuc     1200

gacuuuagcg aucuguacua uuuaaccaug aacaauaaac auuggcuggu gcacaaggaa     1260

ugguuccacg acauuccucu cccauggcau gccggcgcug auacaggaac cccgcacugg     1320

aacaauaaag aggcccuugu cgaauuuaag gacgcacaug ccaagaggca gacugugguc     1380

gugcugggcu cccaggaggg ggcuguucac acggcguugg caggagcccu agaggcugaa     1440

auggauggug ccaaaggccg gcuguucucu gggcaucuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacuca uuguguacag cagccuuuac cuucacuaag     1560

guacccgcug agacccucca cggcacaguc accguggaag uucaguaugc cgguacugac     1620

gggccuugca aggugccagc acaaauggcg gucgauaugc agacacugac gcccgugggc     1680

agguuaauaa ccgcuaaccc uguaaucacu gagaguacag aaaacuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auugugaucg gcguugggga caagaaaaua     1800

acccaccauu ggcaccguuc uggaucaaca auugguaagg ccuucgaggc aacuguccgg     1860

ggcgccaaac gaauggcugu gcucggggau accgccuggg acuucggaag ugugggcggu     1920

gucuuuaaua gccuggggaa gggcauccau cagaucuucg gagcagcuuu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugaucggaa cgcugcucgu uuggcugggc     2040

cuuaacacca aaaaugguag cauuucccug acauguuugg cccucggggg agugaugaua     2100

uuccugucaa cugcggugag ugccgaugua ggcugcucug ucgacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8421
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 3 opt

<400>  8421
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca auuugggggu gaacaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua caaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaauac uaccagcaca uggguagugu acgguaccug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacucuac caucucacuc gacacggaag      420

cuccaaaccc gcucacagac guggcuggag agccgagagu auacuaagca ucuuauaaaa      480

guggaaaacu ggaucuuuag aaauccggga uucgcucugg ccgcgguugc aauugccugg      540

uugcucggca guuccaccuc ucagaaggug aucuaccugg ucaugauucu gcucaucgcu      600

cccgccuaua gcauaaggug cauuggcgug uccaaccggg auuuuguaga ggggauguca      660

ggagguacau ggguugacgu ggucuuagaa cacggcgggu gugugacugu gauggcacag      720

gauaaaccua ccgucgacau cgagcugguu acaaccacgg ugaguaauau ggcugaggua      780

cguagcuacu gcuacgaagc cucuauuucc gauauggcgu cagacagccg cuguccaacu      840

caaggagagg ccuaucuuga caagcagagu gauacacagu acgugugcaa aagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca agggcucucu agugacuugc      960

gcaaaguuua caugcuccaa aaagaugacc gggaaaagca uccagcccga aaaucucgag     1020

uaucggauca ugcugucggu ucacggaucu caacauuccg guaugauugu gaacgauaua     1080

ggccacgaaa cggacgagaa ucgagcuaag gucgagguga ccccuaacuc accacgcgcc     1140

gaagcuacac uugggggauu cggcagucug gguuuggauu gugagcccag aacugggcuc     1200

gacuuuagcg aucuguacua uuuaaccaug aauaacaaac auuggcuggu acacaaggaa     1260

ugguuccacg acaucccucu cccauggcau gccggcgcag auacaggaac uccgcacugg     1320

aauaacaaag aggcccuugu ugaauuuaag gacgcucaug cgaagaggca gaccgugguc     1380

gugcugggcu cccaggaggg ggcaguccac acagccuugg cuggagcccu agaggcagaa     1440

auggauggug ccaaaggccg gcuguucucu gggcaucuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacuca uuguguaccg cugccuuuac uuucacgaag     1560

guucccgcag agacacucca cggcaccgug acuguagaag uccaguaugc ggguaccgac     1620

gggccuugca aggugccagc ucaaauggcc guugauaugc agacacugac ccccgugggc     1680

agguuaauua cugcaaaccc ugucaucaca gagaguacgg aaaauuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auagugauug gcguagggga caagaaaauc     1800

acccaccauu ggcaccguuc uggaucaacu aucgguaagg ccuucgaggc uacagugcgg     1860

ggcgccaaac gaauggcagu ucucggggau accgcuuggg acuucggaag ugucggcggu     1920

guguuuaaca gccuggggaa gggcgugcau cagauuuucg gagccgcguu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauc uugauuggaa cacugcucgu cuggcugggc     2040

cuuaauacua aaaacgguag cauaucccug accuguuugg cccucggggg aguuaugauc     2100

uuccugucaa cggcugugag ugcagaugug ggcugcucug uagacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8422
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 3 opt

<400>  8422
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuauaaaa      480

guggaaaauu ggaucuuuag aaacccggga uucgcucugg uugccguggc gauugcaugg      540

uugcucggca guuccacauc ucagaagguc aucuaccugg ugaugauucu gcucaucgcc      600

cccgcuuaua gcauaaggug cauuggcgua uccaaucggg auuuuguuga ggggauguca      660

ggagguacuu ggguggacgu cguguuagaa cacggcgggu gugugaccgu cauggcccag      720

gauaaaccua caguugacau cgagcuggug accacgacug uaaguaacau ggcagaggug      780

cguagcuacu gcuacgaggc uucuauuucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgucugcaa aagaacuuug      900

guggacaggg gcugggguaa ugggugugga cuguucggca agggcucucu aguuacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uccagcccga aaaccucgag     1020

uaucggauca ugcugucggu gcacggaucu caacauuccg guaugauugu caaugauaua     1080

ggccacgaaa ccgacgagaa ccgagcaaag guggagguaa caccuaauuc accacgcgcu     1140

gaagccacuc uugggggauu cggcagucug gguuuggauu gugagcccag aaccgggcuc     1200

gacuuuagcg aucuguacua uuuaacaaug aacaauaaac auuggcuggu ucacaaggaa     1260

ugguuccacg acaucccucu cccauggcau gcuggcgccg auacuggaac cccgcacugg     1320

aacaauaaag aggcacuugu ggaauuuaag gacgcccaug cuaagaggca gacagucgug     1380

guccugggcu cccaggaggg ggcggugcac accgcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggccg gcuguucucu gggcaucuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu uagcuacuca uuguguacug ccgcuuuuac guucacaaag     1560

gugcccgccg agacccucca cggcacugua accgucgaag ugcaguaugc agguacagac     1620

gggccuugca agguuccagc gcaaauggcu guggauaugc agacccugac ucccgucggc     1680

agguuaauua cagccaaccc ugugaucacg gagaguaccg aaaacuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auaguaauug gcguggggga caagaaaauc     1800

acucaccauu ggcaccguuc uggaucaaca aucgguaagg cauucgaggc caccguucgg     1860

ggcgcuaaac gaauggccgu ccucggggau acagcauggg acuucggaag ugugggcggu     1920

guguuuaaua gccuggggaa gggcauucau cagaucuucg gagcugccuu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugauaggaa cucugcucgu cuggcugggc     2040

cuuaacacca aaaaugguag caucucccug acguguuugg cgcucggggg aguuaugauu     2100

uuccugucaa ccgccgugag ugcugaugug ggcugcucug uagacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8423
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 3 opt

<400>  8423
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca auuugggggu gaacaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua caaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaauac uaccagcaca uggguagugu acgguaccug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gucacucuac caucucacuc gacacggaag      420

cuccaaaccc gcucacagac guggcuggag agccgagagu auacuaagca ucuuauaaaa      480

guggaaaacu ggaucuuuag aaauccggga uucgcucugg ccgcgguugc aauugccugg      540

uugcucggca guuccaccuc ucagaaggug aucuaccugg ucaugauucu gcucaucgcu      600

cccgccuaua gcauaaggug cauuggcgug uccaaccggg auuuuguaga ggggauguca      660

ggagguacau ggguugacgu ggucuuagaa cacggcgggu gugugacugu gauggcacag      720

gauaaaccua ccgucgacau cgagcugguu acaaccacgg ugaguaauau ggcugaggua      780

cguagcuacu gcuacgaagc cucuauuucc gauauggcgu cagacagccg cuguccaacu      840

caaggagagg ccuaucuuga caagcagagu gauacacagu acgugugcaa aagaaccuug      900

gucgacaggg gcugggguaa cgggugugga cuguucggca agggcucucu agugacuugc      960

gcaaaguuua caugcuccaa aaagaugacc gggaaaagca uccagcccga aaaucucgag     1020

uaucggauca ugcugucggu ucacggaucu caacauuccg guaugauugu gaacgaugaa     1080

aaucgagcua aggucgaggu gacgccuaac ucaccacgcg ccgaggcuac ccuuggcggg     1140

uucggaaguc ugggcuugga cugugaaccc agaacagguc ucgauuuuag cgaccuguac     1200

uauuuaacua ugaauaacaa acacuggcug guacauaagg agugguucca cgauauaccu     1260

cucccauggc acgccggggc agacaccggc acaccgcauu ggaauaacaa agaagcccuu     1320

guugaguuua aggaugcuca cgcgaagagg cagacugugg ucgugcuggg aucccaggaa     1380

ggcgcagucc auaccgccuu ggcuggggcc cuagaggcag agauggacgg agccaaaggu     1440

cggcuguucu cuggccaccu caagugccgc cugaaaaugg auaaacuuag acugaaaggg     1500

gugagcuacu cauuguguac agcugccuuu accuucacua agguucccgc agaaacgcuc     1560

cauggaacag ugaccguaga gguccaguau gcgggcacug acgguccuug caaggugcca     1620

gcucaaaugg ccguugacau gcagacccug acacccgugg ggagguuaau caccgcaaac     1680

ccugucauua cugaaaguac agaaaauucc aaaaugaugc uggagcuaga uccacccuuu     1740

ggcgacagcu acaucgugau aggaguaggc gauaagaaaa uuacgcacca cuggcaucgu     1800

ucugggucaa ccaucggaaa ggccuucgag gcuacugugc ggggugccaa acgaauggca     1860

guucucggcg acacagcuug ggauuucggg agugucggag gcguguuuaa cagccugggu     1920

aaggggaucc accagauuuu cggcgccgcg uuuaaguccc uuuucggagg caugucuugg     1980

uuuucgcaaa ucuugauugg gacccugcuc guguggcugg gacuuaauac aaagaacggc     2040

agcauauccc ugacuuguuu ggcccucggu ggggucauga ucuuccuguc aaccgcuguu     2100

agugcagacg ugggaugcuc uguggauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8424
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 3 opt

<400>  8424
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucgcucugg uggccgucgc gaucgcaugg      540

uugcucggca guuccacauc ucagaaggug auuuaccugg uaaugauccu gcucauugcc      600

cccgcuuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguacuu gggucgacgu gguguuagaa cacggcgggu gugucaccgu uauggcccag      720

gauaaaccua caguggacau ugagcuggua accacgacug ugaguaacau ggcagagguc      780

cguagcuacu gcuacgaggc uucuaucucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgugugcaa aagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uucagcccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu caacauuccg guaugaucgu gaaugauacc     1080

ggccacgaaa cagacgagaa ccgagcaaag guagagguua cuccuaauuc accacgcgcu     1140

gaagccaccc uugggggauu cggcagucug gguuuggauu gugagcccag aacagggcuc     1200

gacuuuagcg aucuguacua uuuaacuaug aacaauaaac auuggcuggu gcacaaggaa     1260

ugguuccacg acauuccucu cccauggcau gcuggcgccg auaccggaac accgcacugg     1320

aacaauaaag aggcacuugu cgaauuuaag gacgcccaug cuaagaggca gaccgugguc     1380

gugcugggcu cccaggaggg ggcgguucac acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggccg gcuguucucu gggcaucuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacuca uuguguacgg ccgcuuuuac auucaccaag     1560

guacccgccg agacucucca cggcaccguc acaguggaag uucaguaugc agguaccgac     1620

gggccuugca aggugccagc gcaaauggcu gucgauaugc agacucugac acccgugggc     1680

agguuaauaa cggccaaccc uguaaucacc gagaguacug aaaacuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac auugugaucg gcguugggga caagaaaaua     1800

acacaccauu ggcaccguuc uggaucaacc auugguaagg cauucgaggc cacaguccgg     1860

ggcgcuaaac gaauggccgu gcucggggau acugcauggg acuucggaag ugugggcggu     1920

gucuuuaaua gccuggggaa gggcauccau cagaucuucg gagcugccuu uaagucccuu     1980

uucggcggga ugucuugguu uucgcaaauu uugaucggaa cccugcucgu uuggcugggc     2040

cuuaacacga aaaaugguag cauuucccug accuguuugg cgcucggggg agugaugaua     2100

uuccugucaa cagccgugag ugcugaugua ggcugcucug ucgacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8425
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 3 opt

<400>  8425
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaacacaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucauuc gacccggaag      420

cuccagacgc gcucacagac uuggcuggag agccgagagu auaccaagca ccuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucgcucugg uggccgucgc gaucgcaugg      540

uugcucggca guuccacauc ucagaaggug auuuaccugg uaaugauccu gcucauugcc      600

cccgcuuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguacuu gggucgacgu gguguuagaa cauggcgggu gugucaccgu uauggcccaa      720

gauaaaccua caguggacau ugagcuggua accacgacug ugaguaacau ggcagagguc      780

cguagcuacu gcuacgaggc uucuaucucc gauauggccu cagacagccg cuguccaaca      840

cagggagaag cguaucuuga caagcagagu gauacccagu acgugugcaa aagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uucaacccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu cagcacuccg guaugaucgu gaaugauauu     1080

ggccaugaaa ccgacgagaa ccgagcaaag guagagguua caccuaauuc accacgcgcu     1140

gaagccacuc uugggggauu cggcagucug gguuuggauu gugagcccag aaccgggcuc     1200

gacuuuagcg aucuguacua uuuaacaaug aacaauaaac acuggcuggu gcauaaggaa     1260

ugguuccacg acauaccucu cccauggcac gcuggcgccg auacuggaac cccgcauugg     1320

aacaauaaag aggcacuugu cgaauuuaag gacgcccacg cuaagaggca gacagugguc     1380

gugcugggcu cccaggaggg ggcgguucau accgcauugg ccggagcucu agaggccgaa     1440

auggauggug caaaaggccg gcuguucucu gggcaccuca agugccgccu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacuca uuguguacug ccguaugcac ggcugccaag     1560

guccccgcag agacacucca uggcaccgug acuguugaag ugcaauaugc ggguaccgac     1620

gggccuugua aggucccagc ucagauggcc guggauaugc agacacugac ccccguaggc     1680

agguuaauca cugcaaaccc ugugauuaca gagaguacgg aaaacuccaa aaugaugcug     1740

gagcuagacc cacccuuugg agauagcuac aucguuauag gcgucgggga caagaaaauu     1800

acccaccacu ggcaucguuc uggaucaacu aucgguaagg ccuucgaggc uacagugcgg     1860

ggcgccaaac gaauggcagu gcucggggau accgcuuggg acuuuggaag ugucggcggu     1920

guuuucaaua gccuggggaa gggcauccac caaauuuucg gagccgcguu uaagucccuu     1980

uucggcggga ugucuugguu uucgcagauc uugauuggaa cacugcucgu guggcugggc     2040

cuuaacacua aaaaugguag cauaucccug accugcuugg cccucggggg agugaugauc     2100

uuccugucaa cggcuguaag ugcagauguc ggcuguucug uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8426
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 3 opt

<400>  8426
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacgcaagca uagggaucgu gucccugcuc cuuaccacag ucauggccgc ggaaauuacu      120

cguagaggau cugcuuacua uauguaccug gauaggucag acgccgguaa ggcaaucagu      180

uucguuacga ccuugggcgu gaauaaaugc cacguacaga uuauggauuu agggcauaug      240

ugugacgcca caaugagcua ugagugcccc augcuggaug aaggagugga gccugacgau      300

gucgacuguu ggugcaacac uaccuccaca ugggugguuu acggcaccug ucaccauaag      360

aaaggugaag cucggcgcuc uagaagggca gugacucuac caucgcacuc aacacggaag      420

cuccaaaccc gcagccagac guggcuggag agucgagagu auacuaagca ucuuaucaaa      480

gucgaaaauu ggauauuuag aaacccgggg uucgcccugg uggcuguagc caucgcgugg      540

uugcucggau ccucuaccag ccagaagguu auuuaccugg ugaugauccu gcucauugca      600

cccgccuauu ccaucaggug cauaggcguc ucaaaucggg auuuugugga gggcaugagu      660

gggggaacau ggguggacgu cguuuuagaa cacgguggcu gugugacugu aauggcucag      720

gauaaaccua ccguggacau ugagcugguc acaaccacgg ugagcaacau ggccgagguu      780

cguucuuacu gcuacgaagc auccaucuca gauauggcua gcgacagucg cuguccaacu      840

caaggggagg ccuaucuuga caagcagucu gauacacagu acgugugcaa aagaaccuug      900

gucgacaggg gauggggcaa ugguuguggg cuguucggaa agggcucccu agugacuugc      960

gcgaaguuua caugcagcaa aaagaugacc ggcaaaucga uucagcccga aaaccucgag     1020

uaucggauca ugcugucugu acacgggucc caacauucag gaaugaucgu uaaugaugaa     1080

aaccgagcca agguggaggu cacgccuaau aguccacgcg cagaggcuac ccuugguggc     1140

uucgggagcc ugggauugga cugugaaccc agaacaggcc ucgauuuuuc cgaccuguac     1200

uauuuaacua ugaacaauaa acacuggcug gugcauaagg agugguucca cgauauuccu     1260

cucccauggc acucuggugc cgacaccgaa acaccgcauu ggaacaauaa agaggcucuu     1320

gucgaauuua aggaugccca cgcaaagagg cagacugugg uugugcuggg gagccaggag     1380

ggcgccguac auaccgcuuu ggcgggagca cuagaggccg aaauggacgg cgcuaaaggg     1440

cggcugucaa guggacaccu caagugccgc cugaaaaugg auaaacuuag acugaaaggu     1500

gucuccuaca gcuuguguac agccgcauuc accuuuacua aggugcccgc cgagacgcuc     1560

cauggcacag uuaccgugga aguccaguau gcugggaggg acggaccuug caaggugcca     1620

gcccaaaugg caguagacau gcagacucug acccccgugg gccguuuaau aacagcgaac     1680

ccuguuauca ccgagucuac ugaaaacuca aaaaugaugc uggagcuaga uccacccuuc     1740

ggugacaguu acauugucau cggggugggc gauaagaaaa uaacacacca cuggcaucgg     1800

agcggaucca uuaucggcaa ggcuuuugag gccacggugc gaggggcaaa aagaauggcc     1860

guccucggag acaccgcuug ggauuucggu ucuguuggcg ggguguucaa uucgcuggga     1920

aagggcaucc accagauuuu uggugccgca uucaagagcc uuuuuggggg cauguccugg     1980

uucucacaaa ucuugauugg aacucugcuc guguggcugg gccuuaacac aaagaauggg     2040

aguauaucuc ugaccuguuu ggcucucgga ggcguaauga ucuuucugag cacagccguc     2100

uccgcggacg uggguugcuc aguugauuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8427
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 3 opt

<400>  8427
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucgu gggacugcuc cuuacaacug caauggccgc ggaaauuacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaaucuca      180

uucgccacca cauugggggu caauaaaugc cacguucaga uuauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcgugga gccugacgau      300

guagacuguu ggugcaacac cacaagcacc uggguggucu acgguacuug ucaccauaag      360

aaaggggaag cacggcgcuc cagaagggcc gugacacuac caucucacuc gacccggaag      420

cuccaaacgc gcucacagac uuggcuggag agccgagagu auaccaagca ucuuaucaaa      480

guugaaaauu ggauauuuag aaacccggga uucgcucugg uggccgucgc gaucgcaugg      540

uugcucggca guuccacauc ucagaaggug auuuaccugg uaaugauccu gcucauugcc      600

cccgcuuaua gcaucaggug cauaggcguu uccaaucggg auuuugugga ggggauguca      660

ggagguacuu gggucgacgu gguguuagaa cacggcgggu gugucaccgu uauggcccag      720

gauaaaccua caguggacau ugagcuggua accacgacug ugaguaacau ggcagagguc      780

cguagcuacu gcuacgaggc uucuaucucc gauauggccu cagacagccg cuguccaaca      840

caaggagaag cguaucuuga caagcagagu gauacccagu acgugugcaa aagaacuuug      900

guugacaggg gcugggguaa ugggugugga cuguucggca agggcucucu agugacaugc      960

gccaaguuua ccugcuccaa aaagaugacg gggaaaagca uucagcccga aaaccucgag     1020

uaucggauca ugcugucggu ccacggaucu caacauuccg guaugaucgu gaaugaugaa     1080

aaccgagcaa agguagaggu uaccccuaau ucaccacgcg cugaggccac acuuggcggg     1140

uucggaaguc ugggcuugga cugugaaccc agaacugguc ucgauuuuag cgaccuguac     1200

uauuuaacca ugaacaauaa acacuggcug gugcauaagg agugguucca cgauauuccu     1260

cucccauggc acgcuggggc cgacacaggc acuccgcauu ggaacaauaa agaagcacuu     1320

gucgaguuua aggaugccca cgcuaagagg cagaccgugg ucgugcuggg aucccaggaa     1380

ggcgcgguuc auacagcauu ggccggggcu cuagaggccg agauggacgg agcaaaaggu     1440

cggcuguucu cuggccaccu caagugccgc cugaaaaugg auaaacuuag acugaagggg     1500

gugagcuacu cauuguguac cgccgcuuuu acuuucacga agguacccgc cgaaacacuc     1560

cauggaaccg ucacugugga gguucaguau gcaggcaccg acgguccuug caaggugcca     1620

gcgcaaaugg cugucgacau gcagacacug acccccgugg ggagguuaau aacugccaac     1680

ccuguaauca cagaaaguac ggagaacucc aaaaugaugc uggaacuaga uccacccuuu     1740

ggcgacagcu acauugugau cggaguuggc gauaagaaaa uaacccacca cuggcaucgu     1800

ucugggucaa cuauuggaaa ggcauucgag gccacagucc ggggugcuaa acgaauggcc     1860

gugcucggcg acaccgcaug ggauuucggg agugugggag gcgucuuuaa uagccugggu     1920

aaggggaucc accagaucuu cggcgcugcc uuuaaguccc uuuucggagg caugucuugg     1980

uuuucgcaaa uuuugaucgg gacacugcuc guuuggcugg gacuuaacac uaaaaauggc     2040

agcauuuccc ugaccuguuu ggcgcucggu ggggugauga uauuccuguc aacggccgug     2100

agugcugacg uaggaugcuc ugucgauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8428
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 3 opt

<400>  8428
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca cauugggggu gaauaaaugc cacguccaga ucauggauuu aggacauaug      240

ugugacgcua cuaugaguua ugagugcccc augcuggaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaaa cacggcgcuc cagaaggucu gucucgcuac gguaucacua cacccgcaag      420

cuccaaacgc gaucacagac uuggcuggag agcagagagu auaagaaaca ucuuauaaug      480

guggaaaauu ggaucuuuag gaacccagga uucgcaauug uuaguguggc caucaccugg      540

cugaugggcu ccuugacauc ucagaagguc auuuaccucg ugaugaucgu acugauaguu      600

ccggcuuaca gcauuuccug caucggcgug ucaaaucggg aucuggucga ggggaugagu      660

ggagguacuu ggguggacgu gguccucgaa cacggcgggu guguuaccga gauggcccag      720

gauaaaccca caguggacau ugaguuagua accaugacgg ugagcaacau ggcggaaguc      780

cguucuuauu gcuacgaggc aucccuguca gauauggcca gcgcuagucg cuguccuacu      840

caaggagaac caucucuuga caagcagucc gacacacaga gcgugugcaa aagaaccuug      900

ggcgauaggg guugggggaa uggauguggc aucuuuggca aggggucgcu gguuacuugc      960

ucuaaguuca caugcuguaa aaagaugccc ggaaaaucca uccagccuga gaaccuagaa     1020

uaucggauua ugcucccagu gcacgguuca caacauagug gcaugauagu caaugacauc     1080

gggcacgaga ccgaugagaa ccgagccaag guggaaguaa cgcccaauag cccucgcgca     1140

gaggcuaccc ugggaggcuu ugguucccuu gggcuggacu gcgaaccaag aacaggcuug     1200

gauuucucug accucuacua ucugacuaug aacaauaaac auugguuagu ucacaaggag     1260

ugguuucacg auauuccgcu gcccuggcau gccggagcgg acaccggcac accucacugg     1320

aacaauaaag aagcccucgu ggaguucaag gaugcacaug cuaagaggca gacugucgug     1380

guccuuggga gccaggaggg agccgugcac accgcucugg ccggugcauu ggaagccgag     1440

auggacggcg cuaaagggcg gcuauuuuca ggacaucuga agugucgccu caaaauggau     1500

aagcugagac uuaaaggcgu uaguuacucc cugugcacag cggcauucac cuuuacuaag     1560

gugccagccg aaacguugca cgguacagua accgucgagg ugcagagcgc ugggacugac     1620

ggccccugua agguuccugc ccaaauggca guggacaugc agacccucac accagucgga     1680

aggcugauca ccgccaaccc cgugauaacu gaaucuacag agaacucaaa aaugauguua     1740

gagcuggauc cgccuuucgg cgacaguuau auuguaaucg gggugggaga uaagaaaauc     1800

acgcaccauu ggcaccguag cgguuccacc auuggcaagg cuuuugaagc cacuguucga     1860

ggggcaaaac ggauggcggu ccuaggagac acagcuuggg auuucggcuc uguggguggg     1920

guguucaauu cgcucggcaa gggaauccau cagauuuuug gcgccgcauu caagagccug     1980

uuugggggaa uguccugguu cucacaaaua cuuaucggca ccuugcuggu cuggcucggu     2040

cugaacacua aaaaugggag uauuucucuu acaugccugg ccuugggagg cguuaugauc     2100

uuucucagca ccgcuguguc cgccgacgug gguuguucag uagauuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8429
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 3 opt

<400>  8429
gggagaaagc uuaccaugag aaucauuaac gccaggaagg agcggaaacg ccgaggcgcu       60

gacaccagca uagggaucau uggacugcuc cuuacaacug caauggccgc ggaaaucacg      120

cguagagguu ccgcuuacua uauguaccug gauaggucug acgccggcaa ggcaauuuca      180

uucgccacca caaugggggu gaauaaaugc cacguccaga ucauggauuu gggacauaug      240

ugugacgcua cuaugaguua ugagugcccc auguuagaug aaggcguuga gccugacgau      300

guggacuguu ggugcaacac cacaagcacc uggguagugu acgguacuug ucaccauaag      360

aaaggggaag cacggccauc ccgcagagcc gucacacugc cgucucacuc gaccaggaag      420

cuacaaacgc ggucacagac uuggcucgag agccgcgagu auaccaagca ucugauaaaa      480

guggaaaauu ggauccuucg aaaccccgga uuuacaguug cugcccuggc gauggcaugg      540

uugcucggca auaguacuuc ccagagagug auuuaccugg ucaugauccu gcucauugcc      600

ccugcuuauu cuaucaggug cauaggcgug agcaaccggg auuucguaga ggggaugucc      660

ggagguaccu ggguugacgu ggucuuagaa cacggcgggu gugugacagu gauggcccag      720

gauaagccaa ccgucgacau ugagcugguu acgacuacag ugucaaauau ggcagaggua      780

cguaguuacu gcuacgaagc uagcaucucu gauauggccu ccgacucacg cugucccacc      840

caaggagagg cguaucuuga caaacagagc gauacucagu acgugugcaa gagaacauug      900

gucgacaggg gcugggguaa cgggugugga cuguuuggca aaggcagucu agugaccugc      960

gccaaguuca cgugcucuaa gaaaaugacc gggaagucca uucagccuga aaaucucgag     1020

uaucggauca ugcugagcgu ucacggaucg caacauucug guaugaucgu gaacgauauu     1080

ggccacgaaa cagacgagaa ucgagcaaaa gucgagguga cuccaaacuc accccgcgcu     1140

gaagccaccc uugggggauu uggcucccug gguuuggauu gugagccuag aacagggcuc     1200

gacuucagug aucuguacua uuuaacuaug aauaacaagc auuggcuggu acacaaagaa     1260

ugguuucacg acauaccacu cccguggcau gcuggcgccg auaccggaac accccacugg     1320

aauaacaagg aggcacuugu ugaguucaaa gacgcccaug cuaagaggca gaccgugguc     1380

gugcugggca gccaggaagg ggcgguccac acugcauugg ccggagcucu agaggccgaa     1440

auggauggug caaagggccg gcuguuuucc gggcaucuca aaugccgcau caagauggac     1500

aaacugagac uuaagggagu gucuuacagc cuguguacgg ccgcuuucac auuuucaaaa     1560

guuccugccg agaccuugca cggcacugug accguagaag uccaguaugc agguagggac     1620

gggccaugca aggugcccgc gcaaauggcu guugauaugc agacacucac cccugugggc     1680

cgucugauua cugccaaccc aaucauaaca gagaguacgg aaaauucccg gaugauguua     1740

gagcuggauc ccccguucgg agacagcuac auugucaucg gcguggggga caagaaaauc     1800

acccaccauu ggcaccgauc uggaucaacu auugguaagg cauuugaggc cacaguaaga     1860

ggcgcuaaaa ggauggccgu gcuaggggau accgcauggg acuucggaag uguuggcggu     1920

gucuucaaca gccucgggaa gggcgugcau cagguguuug gagcugccuu caaaucccug     1980

uuuggcggga ugucuugguu cucgcaaauc cuuauuggaa cauugcuggu cuggcucggc     2040

cugaauacua agaacgguag cauaucccuu gcgugucugg cccucggggg agcuaugauc     2100

uuuuugucaa ccgcaguuag ugccgaugug ggcugcucug uggacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  8430
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 3 opt

<400>  8430
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauauugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8431
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 3 opt

<400>  8431
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cgccaagaau ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaacggca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8432
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 3 opt

<400>  8432
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgguuacg agaccgacga agaucgugcg aaggucgagg ugacaccuaa cucaccacgc     1080

gccgaggcaa cucuuggcgg guucggaagu cugggcuugg acugugaacc cagaaccggu     1140

cucgauuuua gcgaccugua uuacuuaaca augaauaaca aacacuggcu gguacauaag     1200

gagugguucc acgauauccc ucucccaugg cacgcugggg ccgacacugg caccccgcau     1260

uggaauaaca aagaagcucu uguugaguuu aaggaugccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucccagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggacg gagcuaaggg ucggcuguuc ucuggccacc ucaagugccg acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucauugugua cugccgcauu uacguucaca     1500

aagguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gacgguccuu gcaaaauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggagauuaa uaacagcaaa uccugucauc acggaaagua ccgagaacuc caagaugaug     1680

cuggaacuag auccacccuu uggcgacagc uacauuguga ucggaguagg cgauaagaaa     1740

auaacucacc acuggcauag gucuggguca acaauuggaa aggcguucga ggcuaccgug     1800

cggggugcca aacgcauggc aguucucggc gacacagccu gggauuucgg gagugucgga     1860

ggcguguuua auagccuggg uaaggggauc caccagaucu ucggcgcugc cuuuaaaucc     1920

cuuuucggag gcaugucuug guuuucgcaa auuuugaucg ggacucugcu cguguggcug     1980

ggacuuaaca ccaagaacgg cagcauuucc cugacguguu uggcacucgg uggggucaug     2040

auauuccugu caaccgcugu uagugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8433
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 3 opt

<400>  8433
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8434
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 3 opt

<400>  8434
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgucaacg acaccgguua cgagacagau gaaaaucgug cgaaggugga gguaacuccu     1080

aacucaccac gcgccgaggc aacccuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacag gucucgauuu uagcgaccug uauuacuuaa cuaugaauaa caaacacugg     1200

cugguucaua aggagugguu ccacgauauc ccucucccau ggcacgcugg ggccgacacc     1260

ggcacaccgc auuggaauaa caaagaagcu cuuguggagu uuaaggaugc ccacgcaaaa     1320

aggcagaccg ucgugguccu gggaucccag gaaggcgccg ugcauacugc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag gguaagcugu ucucuggcca ccucaaaugc     1440

cggcugaaga uggauaaacu ucgacugaag gggguuagcu auucauugug uacggccgca     1500

uuuacauuca ccaaagugcc cgccgaaacu cuccauggaa ccguaacagu cgaggugcag     1560

uacgcuggca ccgacggucc uugcaagauu ccaguucaaa uggccgugga caugcagacu     1620

cugacacccg ucgggcgcuu aauaacggca aauccuguga ucaccgaaag uacugagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu aaucggagug     1740

ggcgauaaaa agauaacaca ccacuggcau agaucugggu caaccauugg aaaagcguuc     1800

gaggcuacag uuaggggugc caagcggaug gcaguccucg gcgacacugc cugggauuuc     1860

gggagugugg gaggcguguu uaacagccug gguaaaggga uccaccagau cuucggcgcu     1920

gccuuuaagu cccuuuucgg aggcaugucu ugguuuucgc aaauuuugau cgggacccug     1980

cucgucuggc ugggacuuaa uacgaagaac ggcagcauuu cccugaccug uuuggcacuc     2040

ggugggguua ugauauuccu gucaacagcu gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8435
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 3 opt

<400>  8435
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc gcaagaaagg ugaggcccga cguuccagaa gggcugucac acuaccaucu      360

cauucgaccc ggaagcucca aacgcgcuca cagacuuggc uggaaagcag agaguauacc      420

aaacaccuua uuaaggugga gaacuggauc uuuaggaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu cggugcaucg gcguauccaa ccgcgacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcaugg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgaag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agcagauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagga cuuuggugga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg uauuaugcug ucggugcacg ggucucaaca cuccggaaug     1020

aucgucaacg acaccgguua cgagacagau gaaaaucgcg cgaaggugga gguaacuccu     1080

aacucaccaa gagccgaggc aacccuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccaggacag gucucgauuu uagcgaccug uauuacuuaa cuaugaauaa caaacauugg     1200

cugguucaca aggagugguu ccaugauauc ccucucccau ggcacgcugg ggccgacacc     1260

ggcacaccgc acuggaauaa caaagaagcu cuuguggagu uuaaggaugc ccaugcaaaa     1320

cggcagaccg ucgugguccu gggaucccag gaaggcgccg ugcacacugc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag gguaagcugu ucucuggcca ucucaaaugc     1440

cgacugaaga uggauaaacu ucgccugaag gggguuagcu auucauugug uacggccgca     1500

uuuacauuca ccaaagugcc cgccgaaacu cuccacggaa ccguaacagu cgaggugcag     1560

uacgcuggca ccgacggucc uugcaagauu ccaguucaaa uggccgugga caugcagacu     1620

cugacacccg ucgggagauu aauaacggca aauccuguga ucaccgaaag uacugagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu aaucggagug     1740

ggcgauaaaa agauaacaca ucacuggcac aggucugggu caaccauugg aaaagcguuc     1800

gaggcuacag uucggggugc caagcgcaug gcaguccucg gcgacacugc cugggauuuc     1860

gggagugugg gaggcguguu uaacagccug gguaaaggga uccaucagau cuucggcgcu     1920

gccuuuaagu cccuuuucgg aggcaugucu ugguuuucgc aaauuuugau cgggacccug     1980

cucgucuggc ugggacuuaa uacgaagaac ggcagcauuu cccugaccug uuuggcacuc     2040

ggugggguua ugauauuccu gucaacagcu gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8436
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 3 opt

<400>  8436
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

accgucaacg acaucgguua cgagacagau gaaaaucgug cgaaggugga gguaacuccu     1080

aacucaccac gcgccgaggc aacccuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacag gucucgauuu uagcgaccug uauuacuuaa cuaugaauaa caaacacugg     1200

cugguucaua aggagugguu ccacgauauc ccucucccau ggcacgcugg ggccgacacc     1260

ggcacaccgc auuggaauaa caaagaagcu cuuguggagu uuaaggaugc ccacgcaaaa     1320

aggcagaccg ucgugguccu gggaucccag gaaggcgccg ugcauacugc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag gguaagcugu ucucuggcca ccucaaaugc     1440

cggcugaaga uggauaaacu ucgacugaag gggguuagcu auucauugug uacggccgca     1500

uuuacauuca ccaaagugcc cgccgaaacu cuccauggaa ccguaacagu cgaggugcag     1560

uacgcuggca ccgacggucc uugcaagauu ccaguucaaa uggccgugga caugcagacu     1620

cugacacccg ucgggcgcuu aauaacggca aauccuguga ucaccgaaag uacugagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu aaucggagug     1740

ggcgauaaaa agauaacaca ccacuggcau agaucugggu caaccauugg aaaagcguuc     1800

gaggcuacag uuaggggugc caagcggaug gcaguccucg gcgacacugc cugggauuuc     1860

gggagugugg gaggcguguu uaacagccug gguaaaggga uccaccagau cuucggcgcu     1920

gccuuuaagu cccuuuucgg aggcaugucu ugguuuucgc aaauuuugau cgggacccug     1980

cucgucuggc ugggacuuaa uacgaagaac ggcagcauuu cccugaccug uuuggcacuc     2040

ggugggguua ugauauuccu gucaacagcu gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8437
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 3 opt

<400>  8437
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua ucaagguuga gaacuggauu uuuagaaauc cgggguucac acugguggca      480

gucgccaucg cuugguugcu cggaaguucc acuucucaga aggugauaua ccugguaaug      540

auccugcuca uugcccccgc guauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aaccuggguc gauguggugu uagagcacgg uggcuguguc      660

acaguuaugg cacaggacaa accuaccgug gauaucgaac ugguaacgac uacagugagu      720

aauauggccg agguccgcag cuacugcuac gaggcuucua uauccgacau ggccucagau      780

agccgauguc caacccaagg ggaagcauau cuugacaagc agagugacac ucaguacgug      840

ugcaaaagaa cauugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga ccugcgcuaa auuuacgugc uccaagaaga ugaccggcaa aagcauucag      960

cccgagaauc ucgaauaucg gaucaugcug ucgguccacg ggucucaaca uuccggaaug     1020

auugugaacg acgagaaucg ugccaaggua gaaguuacac cuaacucacc acgcgcggag     1080

gccacucuug guggcuucgg gagucuggga uuggauugug agcccagaac cggccucgac     1140

uuuagcgauc uguacuauuu aacaaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaca ucccucuccc auggcacgca ggggcugaua cugggacccc gcauuggaau     1260

aacaaagagg cccuugucga auuuaaggac gcucacgcca aaaggcagac aguggucgug     1320

cugggcuccc aggagggagc aguucauacc gccuuggcug gcgcgcuaga agcagagaug     1380

gauggggcca agggacggcu guucucuggu caccucaagu gccgacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucauug uguacugcug ccuuuacguu cacaaaggua     1500

cccgcagaga cccuccaugg gacugucacc guggaaguuc aguaugccgg aacagauggc     1560

ccuugcaaag ugccagcuca aauggccguc gacaugcaga cccugacucc cguggguaga     1620

uuaaucacag caaauccugu aauuacggag aguaccgaaa acuccaagau gaugcuggag     1680

cuagacccac ccuuugggga uagcuacaua gugaucggcg uuggagacaa gaaaauuacu     1740

caccacuggc auaggucugg cucaacaauc gggaaggcgu ucgaagcuac cguccgggga     1800

gccaaacgca uggcagugcu cggugauaca gccugggacu ucggcagugu ggguggaguc     1860

uuuaacagcc ugggcaaggg uauacaccag auuuucgggg cugccuuuaa aucccuuuuc     1920

ggcggaaugu cuugguuuuc gcaaaucuug aucggcacuc ugcucguuug gcuggggcuu     1980

aauaccaaga acggaagcau uucccugacg uguuuggcac ucggcggugu gaugaucuuc     2040

cugucaaccg cugugagugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8438
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 3 opt

<400>  8438
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgagaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgguuacg agaccgacga aaaccgugcg aaggucgagg ugacaccuaa uucaccacgc     1080

gccgaggcaa cucuuggcgg guucggaagu cugggcuugg auugugaacc cagaaccggu     1140

cucgacuuua gcgaucugua uuacuuaaca augaacaaua aacacuggcu gguacauaag     1200

gagugguucc acgacauccc ucucccaugg cacgcugggg ccgauacugg caccccgcau     1260

uggaacaaua aagaagcucu uguugaguuu aaggacgccc acgcaaaaag gcagacagug     1320

gucgugcugg gaucccagga aggcgccguc cauaccgcuu uggcgggggc acuagaggcc     1380

gagauggaug gagcuaaggg uaagcuguuc ucuggccacc ucaaaugccg gcugaagaug     1440

gacaaacuuc gacugaaggg ggugagcuau ucauugugua cugccgcauu uacguucaca     1500

aaaguucccg ccgaaacccu ccauggaacu gugaccguag agguccagua cgcuggcaca     1560

gaugguccuu gcaagauucc agugcaaaug gccguugaca ugcagacccu gacucccgug     1620

gggcgcuuaa uaacagcaaa cccugucauc acggaaagua ccgaaaauuc caagaugaug     1680

cuggaacuag acccacccuu uggcgauagc uacauuguga ucggaguagg cgacaaaaag     1740

auaacucacc acuggcauag aucuggguca acaauuggaa aagcguucga ggcuaccgug     1800

aggggugcca agcggauggc aguucucggc gauacagccu gggacuucgg gagugucgga     1860

ggcguguuua acagccuggg uaaagggauc caccagaucu ucggcgcugc cuuuaagucc     1920

cuuuucggag gcaugucuug guuuucgcaa auuuugaucg ggacucugcu cguguggcug     1980

ggacuuaaca ccaagaaugg cagcauuucc cugacguguu uggcacucgg uggggucaug     2040

auauuccugu caaccgcugu uagugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8439
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 3 opt

<400>  8439
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaggggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaaaaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8440
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 3 opt

<400>  8440
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccgggcuggc uuuggcagcc      480

gcugccauug cguggcuccu gggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacucuuaa ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuc      600

guagagggca uguccggggg aacuuggguu gauguggucc uggaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc uuguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac uuggacaagc agagcgacac ccaguaugug      840

ugcaaaagga cucuggucga ucggggaugg ggcaaugguu gugggcuauu uggaaagggc      900

agucucguga caugcgcgaa auucgccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc uggaguaccg uauuaugcuu agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucugggc ggguuuggau cauugggccu cgacugugag     1140

ccuaggaccg gucuggauuu cagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ucacgauauc ccacuuccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cugguagagu ucaaggaugc ccacgcuaaa     1320

cggcagacag uuguggucuu gggaagccag gaaggcgcgg ugcauaccgc acuagccggg     1380

gcucuggagg ccgaaaugga cggagcaaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gagauugaaa ggggucagcu auucacucug uacugccgcu     1500

uuuacguuca caaagauccc ugccgagacc cugcauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

uuaacuccug uggggaggcu gauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgaucccccg uuuggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuc     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacugg gcgauacagc augggacuuu     1860

gggagugugg gaggcgcucu uaauagcuug gguaagggga uucaccagau cuucggcgcc     1920

gcguucaaau cccuguuugg aggcaugucu ugguucucgc aaauccucau ugggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcaucu cccucaugug ucuggccuua     2040

ggugggguuc ugauuuuucu aucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8441
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 3 opt

<400>  8441
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacugguau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaucagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8442
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 3 opt

<400>  8442
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga cacccagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cacgaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8443
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 3 opt

<400>  8443
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agauccgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8444
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 3 opt

<400>  8444
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cuggcgcaaa uggcugucga caugcagacc     1620

uuaacucccg uggggaggcu gauuacagcc aacccuguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgauccaccc uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacugg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu uaauagcuug gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cccuguucgg aggcaugucu ugguuuucgc aaauccucau ugggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcaucu cccucaugug ucuggccuua     2040

ggugggguuc ugauuuuccu aucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8445
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 3 opt

<400>  8445
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuacccu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuacga ggaaacucca aacucggucg cagaccuggc uggagucacg cgaguacaca      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acuucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aaccuggguu gauguggucu uagaacaugg uggcugugug      660

acagugaugg cucaggacaa gccaaccguc gauauugagc ugguuacgac uacaguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacccaagg ggaggccuac cuugacaagc agagcgacac ucaguaugug      840

ugcaaaagga cauuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga ccugcgcgaa auuugccugc ucuaagaaaa ugacgggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacagau gagaaccgcg caaaggucga gauuacucca     1080

aauuccccca gagcugaagc cacccuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacag gucucgauuu uagugaccug uauuacuuaa cuaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacc     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgagag uacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggucugggu caaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cacgaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8446
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 3 opt

<400>  8446
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacgguc gugagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8447
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 3 opt

<400>  8447
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa gugcaugggu ggucuauggc      300

accugucauc acaaaaaggg ugaagcccgc cgaagccgua gagcugugac ucuaccgucc      360

cauucuacaa ggaaacucca aacccggccc cagacguggc uggagucgcg cgaguacacu      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc cuggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaucaagc accagucaga aagugaucua ucuggucaug      540

auacugcuca ucgccccagc uuacuccauu cggugcaucg gcgugucuaa ucgcgacuuu      600

guagagggca ugagcggggg aacauggguu gauguggucu uagaacaugg uggcugugug      660

acugugaugg cccaggacaa gcccaccguc gauauugagc ugguuacaac cacggugucc      720

aacauggcag agguacgauc auacugcuau gaagcuagua ucagcgacau ggccucugau      780

uccagauguc cuacucaagg ggaggcguac cuugacaagc agucagacac acaguaugug      840

ugcaaaagga ccuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agccuaguga cuugcgccaa auuugcaugc aguaagaaaa ugacaggcaa gucuauacag      960

ccagaaaacc ucgaguaccg uauuaugcug uccguucacg ggagccaaca cucgggaaug     1020

aucgugaaug acaccgguca ugaaacggau gagaaccgcg cuaaggucga gauuaccccc     1080

aauucuccua gagccgaagc uacacuuggc ggguucggau cccugggcuu ggacugugag     1140

ccaaggacug gucucgauuu uucagaccug uauuacuuaa ccaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccgcuccccu ggcacgccgg ggcagacaca     1260

ggcacuccuc auuggaacaa uaaagaggcc cuuguagaau uuaaggaugc ucacgcgaaa     1320

cggcagaccg uugugguccu gggaagucag gagggcgcag ugcauacagc cuuggcuggg     1380

gcccuagagg cagaaaugga cggagccaag ggucgacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaaa ggggucucuu auagcuugug uaccgcugcc     1500

uucacuuuua cgaagauccc agcagagaca cuccauggaa ccgugacugu ugaagugcag     1560

uacgcgggca ccgacggucc cugcaaagua ccugcucaaa uggccgucga caugcagaca     1620

cugaccccag uggggagguu aauuacugca aaccccguua uaacagaguc aacggaaaac     1680

aguaagauga ugcuggagcu agauccgccu uucggcgacu ccuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggagcgggu cuacuauagg aaaggccuuu     1800

gaagcuacag ugcgcggugc caaaagaaug gcaguacucg gcgauaccgc uugggacuuc     1860

gggucagugg gaggcgcccu gaauagucuu gguaagggga uucaccagau cuucggcgcg     1920

gccuuuaaga gcuuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcucgauca gcuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu guccaccgca gucucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8448
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 3 opt

<400>  8448
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acauccaaaa aggugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaggg ggaggccuac cuugacaaac aaagcgacac ccaguaugug      840

ugcaagagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaaaggc      900

agucuaguga caugcgcgaa guuugccugc ucuaaaaaga ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcagca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaagucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaagcacugg     1200

cuggugcaua aagaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaggaggca cuuguagaau uuaaagaugc ccacgcuaag     1320

cggcaaacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaaa ggucgacugu ccucuggcca ccucaagugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaaaauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaggua cccgcgcaga uggcugucga caugcaaacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaga agaucacuca ccacuggcau cggucugggu caacaauagg aaaagcauuu     1800

gaagccaccg ugcgcggugc uaagagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaaaggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc agauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaaaaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8449
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 3 opt

<400>  8449
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acacggcaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacuccauu cgcugcaucg gcgugucuaa ucgagacuuu      600

guagagggca ugagcggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugucc      720

aacauggccg agguaagauc auacugcuau gaagcaagua ucagcgacau ggcuucugau      780

uccagguguc ccacacaagg ggaggccuac cuugacaagc agucagacac ccaguaugug      840

ugcaaacgga cuuuggucga ucguggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agccuaguga caugcgcgaa auuugccugc aguaagaaaa ugaccggcaa gucuauacag      960

ccugaaaacc ucgaguaccg cauuaugcug uccguucacg ggagccaaca cucgggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaacagag caaaggucga gauuacacca     1080

aauucuccca gggcugaagc cacucuuggc ggguucggau cccugggcuu ggacugugag     1140

ccucggaccg gucucgauuu uucagaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cgacagacag uugugguccu gggaagucag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgccuga gcuccggcca ccucaaaugc     1440

agacugaaga uggauaaacu uaggcugaaa ggggucucuu auagcuugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggcgguu aauuacagcc aacccaguua uaacggaguc aaccgaaaac     1680

aguaagauga ugcuggagcu agaucccccg uucggcgacu ccuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cgcagcgggu cuacaauagg aaaggcauuu     1800

gaagccaccg ugagaggugc uaaaaggaug gccguacucg gcgauacagc augggacuuc     1860

gggucagugg gaggcgcucu gaauagucuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaga gcuuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcucgauca gcuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu guccacggcu gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8450
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 3 opt

<400>  8450
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaacccag gagggcgcgg ugcauacugc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaaa ggggucucuu auagcuugug uacggccgcu     1500

uucacauuua ccaagauccc ugccgagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcc aacccaguua uaaccgaguc aacugaaaac     1680

aguaagauga ugcuggagcu agaucccccg uucggcgacu ccuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacaca ccacuggcau cggagcgggu cuaccauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauacugc augggacuuc     1860

gggucagugg gaggcgcucu gaauagucuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaga gcuuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacccuc     1980

cugauguggc uuggacugaa cacgaagaau ggcucgauca gcuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu guccaccgcu gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8451
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 3 opt

<400>  8451
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuuua cggugcauug gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucc uggaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauaucgagc uuguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca uuucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac uuggacaagc agagcgacac ccaguaugug      840

ugcaaaagga cucuggucga ucggggaugg ggcaaugguu gugggcuauu cggaaagggc      900

agucucguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauccag      960

ccugaaaacc uggaguaccg uauaaugcuu agcguucacg ggucgcaaca cucuggaaug     1020

auugugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gaucacacca     1080

aauuccccca gagcugaagc cacucugggc ggguucggau cauugggccu cgacugugag     1140

ccuaggaccg gucuggauuu uagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccacuuccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cugguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uuguggucuu gggaagccag gagggcgcgg ugcauaccgc acuagccggg     1380

gcucuggagg ccgaaaugga cggagcaaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gagauugaaa ggggucagcu auucacucug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cugcauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

uuaacuccug uggggaggcu gaucacagcc aacccaguua uuacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgaucccccg uucggcgaca gcuacauagu gaucggaguc     1740

ggcgagaaaa agauuacuca ccacuggcau cggucugggu caacaaucgg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacugg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu uaauagcuug gguaagggga uacaccagau uuucggcgcc     1920

gcguuuaaau cccuguucgg aggcaugucu ugguuuucgc aaauccucau cgggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcauuu cccucaugug ucuggccuua     2040

ggugggguuc ugaucuuccu aucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8452
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 3 opt

<400>  8452
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaaucga aaauuggauu uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccaucg cgugguugcu cggaagcagu acaucccaga aaguuauaua ucuggugaug      540

auccugcuca uugcacccgc cuacucuauc cggugcauug gcgucagcaa ucgcgacuuu      600

guggagggca uguccggggg aacuugggua gauguugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagccag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagcc aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

gguggggugu uaauauuccu gucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8453
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 3 opt

<400>  8453
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc cugaggaacc ccggguuugc uuuggcagcc      480

gcugccauug cguggcuccu gggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacucuuaa ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuc      600

guagagggca uguccggggg aacuuggguu gauguggucc uggaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc uuguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac uuggacaagc agagcgacac ccaguaugug      840

ugcaaaagga cucuggucga ucggggaugg ggcaaugguu gugggcuauu uggaaagggc      900

agucucguga caugcgcgaa auucgccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc uggaguaccg uauuaugcuu agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucugggc ggguuuggau cauugggccu cgacugugag     1140

ccuaggaccg gucuggauuu cagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ucacgauauc ccacuuccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cugguagagu ucaaggaugc ccacgcuaaa     1320

cggcagacag uuguggucuu gggaagccag gaaggcgcgg ugcauaccgc acuagccggg     1380

gcucuggagg ccgaaaugga cggagcaaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gagauugaaa ggggucagcu auucacucug uacugccgcu     1500

uuuacguuca caaagauccc ugccgagacc cugcauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

uuaacuccug uggggaggcu gauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgaucccccg uuuggcgaca gcuacaucgu gauuggaauc     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuc     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcugg gcgauacagc augggacuuu     1860

gggaguguag gaggcgcucu uaauagcuug gguaagggga uccaccagau cuucggcgcc     1920

gcguucaaau cccuguuugg aggcaugucu ugguucucgc aaauucucau cgggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcauuu cccucaugug ucuggccuua     2040

gguggggugc ugauauuucu aucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8454
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 3 opt

<400>  8454
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuucaac cacggugagu      720

aacauggccg agguacgaag cuacugcuau gaagcaacua ucucugacau agcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcguucacg ggucgcaaca cucuggaaug     1020

auugugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag cgguugugcu gggaagccag gagggcgcag uccauaccgc cuuggcuggg     1380

gcccuagagg cagaaaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcugcc     1500

uucacguuua caaagauucc ugcagagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcgggca cagacggucc augcaaagug cccgcucaaa uggccguaga caugcagacc     1620

cugacuccug ucgggagguu aauaacagca aacccaguga ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu uaucggagug     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggccuuu     1800

gaagcuaccg uccgcggugc caaaagaaug gcagugcucg gcgauacagc uugggacuuc     1860

gggaguguag gaggcgcccu gaauagccuu gguaagggga uccaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

gguggggugu uaauauuccu gucaacggca guuagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8455
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 3 opt

<400>  8455
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaacugcag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

uuaacuccug ucgggaggcu gauuacagcc aacccaguga uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgaucccccg uucggcgaca gcuacaucgu uauuggagug     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcugg gcgauacagc augggacuuc     1860

gggaguguag gaggcgcucu uaauagcuug gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cccuguucgg aggcaugucu ugguuuucgc aaauccucau ugggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcaucu cccucaugug ucuggccuua     2040

gguggggugc ugauuuuccu aucaacggcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8456
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 3 opt

<400>  8456
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaucgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu gaucggaguc     1740

ggcgagaaaa agauaaccca ccacuggcau cggucugggu caacuauugg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauaccgc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

ggugggguuu uaauauuccu gucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8457
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 3 opt

<400>  8457
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagucaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaguu aagacugaaa ggggugagcu auucauugug uacugcagcc     1500

uucacguuua caaagauccc ugcugagacc cuccauggaa cuguuaccgu ggaaguacag     1560

uacgccggca cagacggucc augcaaaguc cccgcacaaa uggcggugga caugcagacc     1620

cugacuccug uugggagguu aauuacagcu aacccaguga uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu cauuggagug     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggccuuu     1800

gaagcaaccg uacgcggugc caaaagaaug gcugugcucg gcgauacagc cugggacuuc     1860

gggaguguug gaggcgcacu gaauagccuu gguaagggga uucaccagau cuucggcgcu     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuucaugug uuuggcgcuc     2040

gguggggucc ugauuuuucu uucaacggcc gugagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8458
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 3 opt

<400>  8458
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag gguaaacugu ccucuggcca ccucaagugc     1440

cgacugaaga uggauaaacu ucgccugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaaaauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaggua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagauu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaga agaucacuca ccacuggcau aggucugggu caacaauagg aaaagcauuu     1800

gaagccaccg ugcggggugc uaagcgcaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaaaggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaaaaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8459
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 3 opt

<400>  8459
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaaggaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaca aaugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa guaccugggu ggucuauggc      300

acuugucauc acaagaaagg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaagcucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aaacaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aggugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ccgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aauauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaaac agagcgacac ccaguaugug      840

ugcaagagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaaaggc      900

agucuaguga caugcgcgaa guuugccugc ucuaaaaaga ugaccggcaa guccauacag      960

ccugaaaauc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaagucga gauuacacca     1080

aacuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaauaa caagcacugg     1200

cuggugcaua aagaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaggaggca cuuguagaau uuaaagaugc ccacgcuaag     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaaa ggucgacugu ccucuggcca ccucaagugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaaaauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaggua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aauccaguua uaacggagag uaccgaaaac     1680

uccaaaauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaga agaucacuca ccacuggcau cggucugggu caacaauagg aaaagcauuu     1800

gaagccaccg ugcgcggugc uaagagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaacagccuu gguaaaggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa uaccaaaaac ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8460
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 3 opt

<400>  8460
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaggc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagaag ccgagaugga uggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggacaaacu uagacugaaa ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgaaacc cuccauggaa cugugaccgu ugaggugcag     1560

uacgcaggca cagauggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggaaag uaccgagaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgaaaaga agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaggccaccg ugcgcggugc uaaaagaaug gccguacucg gcgacacagc augggauuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8461
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 3 opt

<400>  8461
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cgcucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacug gucucgauuu uagugaccug uauuacuuaa ccaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacaca     1260

ggcacucccc auuggaacaa uaaagaggcc cuuguagaau uuaaggaugc ucacgcgaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcag ugcauacagc cuuggcuggg     1380

gcccuagagg cagaaaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uaccgcugcc     1500

uucacuuuua cgaagauccc ugcagagaca cuccauggaa ccgugacugu ugaagugcag     1560

uacgcgggca ccgacggucc augcaaagua cccgcucaaa uggccgucga caugcagaca     1620

cugaccccug uggggagguu aauuacugca aacccaguua uaacagagag uacggaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggucugggu caacuauagg aaaggccuuu     1800

gaagcuacag ugcgcggugc caaaagaaug gcaguacucg gcgauaccgc uugggacuuc     1860

gggagugugg gaggcgcccu gaauagccuu gguaagggga uucaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaacacugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

ggugggguuu uaaucuuccu gucaacggca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8462
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 3 opt

<400>  8462
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggcg cggacggucc augcaaagua cccgcucaaa uggccgucga caugcagaca     1620

cugaccccug uggggagguu aauuacugca aacccaguua uaacagagag uacggaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggucugggu caacuauagg aaaggccuuu     1800

gaagcuacag ugcgcggugc caaaagaaug gcaguacucg gcgauaccgc uugggacuuc     1860

gggagugugg gaggcgcccu gaauagccuu gguaagggga uucaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8463
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 3 opt

<400>  8463
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcauuagc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucc uggaacaugg uggcugugug      660

accgugaugg cucaggacaa gccuacaguc gauauugagc uuguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc caacacaagg ggaggccuac uuggacaagc agagcgacac ccaguaugug      840

ugcaaaagga cucuggucga ucggggaugg ggcaaugguu gugggcuauu cggaaagggc      900

agucucguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

cccgaaaacc uggaguaccg uauuaugcuu agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacaccu     1080

aauuccccaa gagcugaagc cacucugggc ggguucggau cauugggccu cgacugugag     1140

cccaggaccg gucuggauuu uagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauc ccucuuccau ggcacgcugg ggccgacacu     1260

ggcaccccgc auuggaacaa uaaagaggca cugguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uuguggucuu gggaagccag gagggcgcgg ugcauaccgc acuagccggg     1380

gcucuggagg ccgaaaugga cggagcaaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gagauugaaa ggggucagcu auucacucug uacugccgcu     1500

uucacguuua caaagauccc cgccgagacc cugcauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc uugcaaagua ccagcgcaaa uggcugucga caugcagacc     1620

uuaacucccg uggggaggcu gauuacagcc aacccuguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuagagcu cgauccaccc uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacugg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu uaauagcuug gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cccuguucgg aggcaugucu ugguuuucgc aaauccucau ugggacucug     1980

cuuauguggc ugggauugaa caccaagaau ggcagcaucu cccucaugug ucuggccuua     2040

ggugggguuc ugauuuuccu aucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8464
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 3 opt

<400>  8464
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau gguguccgau      780

ucaagauguc ccacacaagg ggaggcuuac cuugacaagc agagcgacac ccaguauguc      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguua caugcgccaa auuugcgugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

aucgucaaug acacggguca ugaaaccgau gagaaccgcg ccaaggugga gauuacacca     1080

aauuccccca gagcagaagc uacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguacaua aggaaugguu ccacgauauc ccacucccgu ggcacgccgg ggcugacacu     1260

ggcaccccuc auuggaacaa uaaagaggcc cuuguugaau uuaaggaugc acacgccaaa     1320

cggcagacag uggucgugcu gggaagccag gagggcgcug uccauaccgc guuggcaggg     1380

gcccuagagg cugaaaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugagcu auucauugug uacugcagcc     1500

uucacguuua caaagauccc ugcugagacc cuccauggaa cuguuaccgu ggaaguacag     1560

uacgccggca cagacggucc augcaaaguc cccgcacaaa uggcggugga caugcagacc     1620

cugacuccug uugggagguu aauuacagcu aacccaguga uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu cauuggagug     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggccuuu     1800

gaagcaaccg uacgcggugc caaaagaaug gcugugcucg gcgauacagc cugggacuuc     1860

gggaguguug gaggcgcacu gaauagccuu gguaagggga uucaccagau cuucggcgcu     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcgcug     2040

gguggggucu uaauuuuccu gucaacggcc gugagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8465
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 3 opt

<400>  8465
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcauuagcaa ucgcgacuuu      600

guggagggca uguccggggg aacuugggua gauguugugu uagaacaugg uggcuguguc      660

accgugaugg cucaggacaa gccaacagug gauaucgagc uggucaccac gacuguuuca      720

aacauggccg aggugcgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

auuguuaaug acacggguca ugaaaccgau gagaaccgcg caaaggugga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggaa uccugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uucagaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauu ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uaguugugcu gggaagucag gagggcgcgg uccauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaaa ggggugucuu auagcuugug uacugccgcu     1500

uucacguuua caaagauacc ugccgagacc cuccauggaa cugucaccgu ggaaguucag     1560

uacgcaggca cagacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagacc     1620

cugacuccug ucgggagguu aaucacagcc aacccaguga uuacggaguc aaccgaaaac     1680

aguaagauga ugcuggagcu agaucccccg uucggcgacu ccuacaucgu uauaggagug     1740

ggcgagaaaa agauuacuca ccacuggcau cggagcgggu cuacaaucgg aaaggcauuu     1800

gaagccaccg uccgcggugc uaaaagaaug gccgugcucg gcgauacagc augggacuuc     1860

gggucaguag gaggcgcucu gaauagucuu gguaagggga uccaccagau uuucggcgcc     1920

gcguuuaaga gcuuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcucgauca gcuugaugug ucucgcccug     2040

gguggggugu uaauauuccu guccacggcu guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8466
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 3 opt

<400>  8466
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acacuuguga ugccaccaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacacaacca guacuugggu ggucuauggc      300

acaugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ccuaccgucc      360

cauucuauua ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua ucagaguuga aaauuggauu uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccaucg cgugguugcu cggaagcagu acaucccaga aagugauaua ucuggucaug      540

auccugcuca uugcacccgc cuacucuauc cggugcauug gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauaucgagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca uaucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauucag      960

ccugaaaacc ucgaguaccg uaucaugcug agcguucacg ggucgcaaca cucuggaaug     1020

auugugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauucc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauaacagcc aacccaguua ucacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauugu gaucggaguc     1740

ggcgagaaaa agauaacuca ccacuggcau cggucugggu caacaauugg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uccaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauucugau cgggacucuc     1980

cugauguggc uuggacugaa cgccaagaau ggcagcauuu ccuugaugug ucucgcucug     2040

ggugggguuu uaauauuccu gucaaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8467
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 3 opt

<400>  8467
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacaa cgcagccgac gugccgugac acuaccgucc      360

cauucuacca gaaaacucca gacgaggucg cagacuuggc uggagucacg ggaguacacc      420

aagcaccuua uucgcguuga aaauuggauc uuuagaaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu aggugcaucg gcgugagcaa ucgggacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaagacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgcag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucacgauguc ccacacaggg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagaa cuuuggucga uaggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacaa      960

ccugaaaacc ucgaguaccg gauuaugcug agcguucacg ggucgcagca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgug caaaggucga gauuacacca     1080

aauuccccuc gcgcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuagaaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcacacccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

aggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucggcugu ccucuggcca ccucaaaugc     1440

cgacugaaga uggauaaacu ucgccugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcaa     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaga uggcugucga caugcagacc     1620

cugacuccug uggggagauu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau aggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcggggugc uaaacgcaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccaaau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc agauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8468
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 3 opt

<400>  8468
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca agcucggucg cagacguggc uggagucacg cgaguacacu      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc acuggccgcu      480

gccgcgauug caugguugcu cggaagcagu accucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc uuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacauggguu gauguggucu uagaacaugg uggcugugug      660

acugugaugg cccaggacaa gccaaccguc gauauugagc ugguuacaac cacgguguca      720

aacauggcag agguacgaag uuacugcuau gaagcuagca ucucugacau ggccuccgau      780

ucaagauguc ccacucaagg ggaggcguac cuugacaagc agagcgacac acaguaugug      840

ugcaaaagga ccuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga cuugcgccaa auuugcaugc ucuaagaaaa ugacaggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacggau gagaaccgcg cuaaggucga gauuacccca     1080

aauuccccca gagccgaagc uacacuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacug gucucgauuu uagugaccug uauuacuuaa ccaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgccgg ggcagacaca     1260

ggcacucccc auuggaacaa uaaagaggcc cuuguagaau uuaaggaugc ucacgcgaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcag ugcauacagc cuuggcuggg     1380

gcccuagagg cagaaaugga cggagccaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uaccgcugcc     1500

uucacuuuua cgaagauccc ugcagagaca cuccauggaa ccgugacugu ugaagugcag     1560

uacgcgggca ccgacggucc augcaaagua cccgcucaaa uggccgucga caugcagaca     1620

cugaccccug uggggagguu aauuacugca aacccaguua uaacagagag uacggaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggucugggu caacuauagg aaaggccuuu     1800

gaagcuacag ugcgcggugc caaaagaaug gcaguacucg gcgauaccgc uugggacuuc     1860

gggagugugg gaggcgcccu gaauagccuu gguaagggga uucaccagau cuucggcgcg     1920

gccuuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcagcaucu ccuugaugug ucucgcucug     2040

ggugggguuu uaauuuuccu gucaaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8469
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 3 opt

<400>  8469
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacagcauu cggugcaucg gcguguccaa ccgcgacuuu      600

guagagggca ugucaggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugagu      720

aauauggccg agguacgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacacaagg ggaggccuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcgaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacucaccca gagcugaagc cacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaucccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaaa ggggucucau auaguuugug uacugccgcu     1500

uucacguuuu ccaagauccc ugccgagaca cuccauggaa ccgugacuga agagguucag     1560

uacgcaggca ccgacggucc augcaaagug cccgcgcaaa uggcuguaga caugcagaca     1620

cugaccccug ucgggagguu aauuacugcc aauccaguga uaacagaaag cacggagaac     1680

ucuaagauga ugcuggagcu agaucccccg uucggcgacu cauacaucgu uauuggagug     1740

ggcgaaaaga agaucaccca ccacuggcau cggaguggga gcacuauagg aaaggcauuu     1800

gaggccacag uccgcggugc uaaaagaaug gccgugcucg gcgauaccgc augggacuuc     1860

ggguccguag gaggcgcucu gaacucucuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cguuguucgg aggcaugagc ugguuuuccc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa uacuaagaac ggcucaauca guuugaugug ucucgcccug     2040

gguggggugu uaauuuuccu gucuaccgcu guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8470
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 3 opt

<400>  8470
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacagcauu cggugcaucg gcguguccaa ccgcgacuuu      600

guagagggca ugucaggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugagu      720

aauauggccg agguacgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacacaagg ggaggccuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcgaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacucaccca gagcugaagc cacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacacugg ggcugacacc     1260

ggcacacccc auuggaauaa caaagaggcc cuuguagaau uuaaggaugc acacgccaaa     1320

cggcagaccg uugugguccu gggaucccag gagggcgcug ugcauacugc guuggcaggg     1380

gcccuagagg cugaaaugga cggagccaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gagacugaaa ggggucucau auaguuugug uacggcagcc     1500

uucacauuua ccaagauccc ugcugagacu cuccauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacggucc augcaaagua cccgcacaaa uggcggucga caugcagacu     1620

cugacaccug uggggagguu aauuacggcu aauccaguua uaaccgaggg cacugaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggagaca gcuacaucgu gauuggcguc     1740

ggggagaaaa agaucacaca ccacuggcau cggucuggau caaccauagg uaaggccuuu     1800

gaagcaacag ugcgcggcgc caaaagaaug gcuguacucg gggauacugc cugggacuuc     1860

ggaagugugg gcgguguucu gaacagccuu gggaagggca uucaccagau cuucggagca     1920

gcuuuuaaau ccuuguucgg cgggaugucu ugguuuucgc aaauccugau uggaacccuc     1980

cugauguggc uuggccugaa uacgaagaac gguagcaucu ccuugaugug ucucgcccuu     2040

gggggagucu uaauuuuccu gucaaccgcg gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8471
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 3 opt

<400>  8471
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacagcauu cggugcaucg gcguguccaa ccgcgacuuu      600

guagagggca ugucaggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugagu      720

aauauggccg agguacgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacacaagg ggaggccuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcgaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacucaccca gagcugaagc cacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaucccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucucau auaguuugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aauccaguua uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucaggga guacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagcgugg gaggcgcucu gaacucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa uaccaagaac ggcuccaucu cauugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gaguacggcu gucucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8472
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 3 opt

<400>  8472
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggaaacgca uacuauaugu accuggaccg guccgaugcc      120

ggugaagcga uuucuuuccc caccacaaug ggcaugaaua agugcuauau acagaucaug      180

gacuuggggc acauguguga ugcuacuaug ucauacgagu gcccuauguu agacgaagga      240

guagagccag augacgugga uuguuggugc aacaccacaa guaccugggu cguguauggc      300

acuugucauc acaaaaaggg ugaagcccgc cgaagccgua gagcaguuac acugccgucc      360

cauucuacca ggaaacuaca aacgcggucg cagacuuggc ucgagucacg cgaguacacc      420

aagcaccuga uuagagugga aaauuggauc uuuaggaacc ccggguucgc ccuugcugca      480

gccgcuauug ccuggcuguu gggaagcagu acaucccaga aagucaucua ucucgugaug      540

auacugcuga ucgcgccugc auacucuauu cggugcaucg gcguaagcaa ucgcgacuuu      600

guugagggca uguccggggg aacuugggug gaugucgugc ucgaacaugg uggcugugug      660

accgucaugg cccaggacaa gccaacaguu gauauugagu uagugaccac gacuguauca      720

aacauggcug aggugcgaag uuacugcuau gaagccagca ucucugacau ggcauccgau      780

ucaagauguc ccacacaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguc      840

ugcaaaagga cucuugugga ucggggaugg ggcaaugguu guggguuguu cggaaagggc      900

agucugguua caugcgccaa auuugcgugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc uagaguaccg uauuaugcuc agcgugcacg ggucgcaaca cucuggaaug     1020

aucgucaaug acacggguca ugaaaccgau gagaaccgcg ccaaggugga gauuacacca     1080

aauuccccca gagcagaagc uacucugggc ggguucggau cacuuggccu ggacugugag     1140

ccuaggaccg guuuggauuu uagugaccuc uauuaccuga caaugaacaa uaaacacugg     1200

uuaguacaua aggaaugguu ccacgauauc ccacugccgu ggcacgccgg ggcugacacu     1260

ggcacacccc auuggaacaa uaaagaggcc cucguugaau uuaaggaugc acacgccaaa     1320

cggcagaccg uggucgugcu uggaagccag gagggcgcug uccauaccgc gcuggcaggg     1380

gccuuggagg cugaaaugga cggagccaag ggucgacuau ccucuggcca ccugaaaugc     1440

cgccucaaga uggauaagcu gagacuuaaa ggggugagcu auucacugug uacugcagcc     1500

uucacguuua caaagauccc ugcugagacc uugcauggaa cuguuaccgu ggaaguacag     1560

uacgccggca cagacggucc augcaaaguc cccgcacaaa uggcggugga caugcagacc     1620

cucacuccug uugggaggcu gauuacagcu aacccaguga uaacggagag uaccgaaaac     1680

uccaagauga uguuagagcu ggaucccccg uucggcgaca gcuacaucgu cauuggagug     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggccuuu     1800

gaagcaaccg uacgcggugc caaaagaaug gcugugcuag gcgauacagc cugggacuuc     1860

gggaguguug gaggcgcacu caauagccug gguaagggga uucaccagau cuucggcgcu     1920

gccuuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaaucuugau ugggacucug     1980

cucgucuggc ugggacuuaa caccaagaau ggcagcaucu cccugaugug uuuggcgcuc     2040

gguggggugc ugauuuucuu aucaacggcc gugagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8473
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 3 opt

<400>  8473
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacagcauu cggugcaucg gcguguccaa ccgcgacuuu      600

guagagggca ugucaggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacugugagu      720

aauauggccg agguacgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacacaagg ggaggccuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcgaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguucacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg caaaggucga gauuacacca     1080

aacucaccca gagcugaagc cacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaauaa caaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaucccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucucau auaguuugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aauccaguua uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucaggga guacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagcgugg gaggcgcucu gaacucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa uaccaagaac ggcuccaucu cauugguuug ucucgcccug     2040

gguggggucu uaauuuuccu gaguacggcu gugucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8474
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 3 opt

<400>  8474
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

ggugaagcua uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaucccgua gagccgugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagaguuga aaacuggauc uuuaggaauc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaaguucc acaucucaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacagcauu cggugcaucg gcgugaacaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcaucu ccuugguuug ucucgcccug     2040

gguggggucu uaauuuuccu gucaacggcu gugagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8475
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 3 opt

<400>  8475
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggauccgca uacuauaugu accuggaccg gucugaugcc      120

ggugaagcga uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcuacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guagagccag augacgugga uuguuggugc aauaccacaa gcaccugggu cguguauggc      300

acuugucauc acaaaaaggg ugaagcccgc cgaucccgua gagcaguuac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagagugga aaacuggauc uuuaggaauc ccggguucgc ccuggcugca      480

gccgcuauug ccugguugcu cggaaguucc acaucucaga aagucaucua ucuggugaug      540

auacugcuca ucgcgccugc auacagcauu cggugcaucg gcguauccaa ccgcgacuuu      600

guugagggca ugucaggggg aacuugggug gaugucgugu uagaacaugg uggcugugug      660

accgucaugg cccaggacaa gccagcuguu gauauugagc uggugacaac cacgguaagu      720

aauauggccg aggugcgaag cuacugcuau gaagcaucua ucuccgacau ggcuucagau      780

agcagauguc ccacucaagg ggaggccuac cuugacaagc agagugacac acaguauguc      840

ugcaaaagga ccuuggugga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua cuugcgcgaa auuugccugc uccaagaaaa ugacaggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucggugcacg ggucucaaca cuccggaaug     1020

aucgucaacg acaccgguca ugaaacggau gagaaucgcg caaaggugga gauuacccca     1080

aacucaccca gagcugaagc cacacuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggacug gucucgauuu uagcgaccug uauuacuuaa ccaugaauaa caaacacugg     1200

cugguacaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacaca     1260

ggcacucccc auuggaauaa caaagaggca cuuguugaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uggucgugcu gggaucccag gagggcgcgg uccauacagc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggugucau auaguuugug uaccgccgcu     1500

uucacuuuua cgaagauccc ugccgagaca cuccauggaa ccguuacugu ggaaguacag     1560

uacgcaggca ccgacggucc augcaaaguc cccgcgcaaa uggcugugga caugcagaca     1620

cugaccccug uugggagguu aauuacugcc aauccaguga uaacagaguc cacggaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu cauuggagug     1740

ggcgagaaaa agaucaccca ccacuggcau cggucaggga guacuauagg aaaggcauuu     1800

gaagccacag uacgcggugc uaaaagaaug gccgugcucg gcgauaccgc augggacuuc     1860

gggagcguug gaggcgcucu gaacucccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacacuc     1980

cuggucuggc uuggacugaa uacuaagaac ggcuccaucu cauugaccug ucucgcccug     2040

gguggggugu uaauuuuccu gaguacggcu gugucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8476
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 3 opt

<400>  8476
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggauccgca uacuauaugu accuggaccg gucugaugcc      120

ggugaagcga uuucauuccc caccacauug ggcaugaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcuacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guagagccag augacgugga uuguuggugc aauaccacaa gcaccugggu cguguauggc      300

acuugucauc acaaaaaggg ugaagcccgc cgaucccgua gagcaguuac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagagugga aaacuggauc uuuaggaauc ccggguucgc ccuggcugca      480

gccgucauug cuugguugcu cggaaguucc acaucucaga aagugaucua ucugguaaug      540

auacugcuca ucgccccugc guacagcauu cggugcaucg gcguuuccaa ccgcgacuuu      600

guggagggca ugucaggggg aacuuggguc gauguggugu uagaacaugg uggcuguguc      660

accguuaugg cacaggacaa gccaacagug gauauugagc ugguaaccac gacugugagu      720

aauauggccg agguccgaag cuacugcuau gaggcuucua ucuccgacau ggccucagau      780

agcagauguc ccacacaagg ggaagcauac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuugguuga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgcuaa auuugccugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguccacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg cgaagguaga gauuacacca     1080

aacucaccca gagccgaagc aacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cugguucaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcacacccc auuggaauaa caaagaggcu cuuguggaau uuaaggaugc ccacgcaaaa     1320

cggcagaccg ucgugguccu gggaucccag gagggcgccg ugcauaccgc uuuggcgggg     1380

gcacuagagg ccgaaaugga cggagcuaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag gggguuucau auaguuugug uacugccgca     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu agaaguccag     1560

uacgcuggca cagacggucc augcaaagug cccgcccaaa uggcaguuga caugcagacc     1620

cugacuccug uggggagguu aauuacagcg aauccaguca uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu gauuggagua     1740

ggcgagaaaa agaucacuca ccacuggcau cggucaggga guacaauagg aaaggcuuuu     1800

gaagccaccg ugcgcggugc aaaaagaaug gccguucucg gcgauacagc uugggacuuc     1860

gggagcgucg gaggcgcccu gaacucccuu gguaagggga uucaccagau cuucggcgca     1920

gcuuuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacucuc     1980

cugguguggc uuggacugaa uaccaagaac ggcuccaucu cauugacgug ucucgcccug     2040

gguggggugu uaauuuuccu gaguaccgcg gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8477
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 3 opt

<400>  8477
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggauccgca uacuauaugu accuggaccg gucugaugcc      120

ggugaagcga uuucauuccc caccacauug ggcguaaaca agugcuauau acagaucaug      180

gacuuagggc acauguguga ugcuacuaug aguuacgagu gcccuaugcu ggacgaagga      240

guggagccag augacgucga uuguuggugc aauaccacaa gcaccugggu gguuuauggc      300

acuugucauc acaaaaaggg ugaagcccgc cgaucccgua gagcagugac acuaccgucu      360

cauucgacca ggaaacucca aacgcgguca cagacuuggc uggagagccg cgaguacacc      420

aagcaccuua uuagagucga aaacuggauc uuuaggaauc ccggguucgc ccuggcugca      480

gccgcuauug ccugguugcu cggaaguucc acaucucaga aagugaucua ucugguaaug      540

auacugcuca ucgcgccugc auacagcauu cggugcaucg gcguuuccaa ccgcgacuuu      600

guggagggca ugucaggggg aacuuggguc gauguggugu uagaacaugg uggcuguguc      660

accguuaugg cccaggacaa gccaacagug gauauugagc ugguaaccac gacugugagu      720

aauauggcug agguccgaag cuacugcuau gaagccucua ucuccgacau ggcaucagau      780

agcagauguc ccacacaagg ggaggcuuac cuugacaagc agagugacac ccaguaugug      840

ugcaaaagga cuuugguuga ucggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgccaa auuugcgugc uccaagaaaa ugaccggcaa gagcauacag      960

ccugaaaauc ucgaguaccg uauuaugcug ucgguccacg ggucucaaca cuccggaaug     1020

aucgugaacg acacggguca ugaaaccgau gagaaucgcg ccaagguaga gauuacacca     1080

aacucaccca gagcagaagc uacucuuggc ggguucggaa gucugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagcgaccug uauuacuuaa caaugaauaa caaacacugg     1200

cugguucaua aggaaugguu ccacgauauc ccacucccgu ggcacgccgg ggcugacacu     1260

ggcacacccc auuggaauaa caaagaggcc cuuguggaau uuaaggaugc acacgccaaa     1320

cggcagaccg ucgugguccu gggaucccag gagggcgcug ugcauaccgc guuggcaggg     1380

gcccuagagg cugaaaugga cggagccaag ggucgacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag gggguuucau auaguuugug uacugcagcc     1500

uucacguuua caaagauccc ugcugagacc cuccauggaa cugugaccgu agaaguccag     1560

uacgccggca cagacggucc augcaaagug cccgcacaaa uggcgguuga caugcagacc     1620

cugacuccug uggggagguu aauuacagcu aauccaguca uaacggaguc caccgaaaac     1680

agcaagauga ugcuggagcu agaucccccg uucggcgacu cuuacaucgu gauuggagua     1740

ggcgauaaaa agaucacuca ccacugguau cggucaggga guacaauagg aaaggccuuu     1800

gaggcaaccg ugcgcggugc caaaagaaug gcuguucucg gcgacacagc cugggauuuc     1860

gggagcgucg gaggcgcacu gaacucccuu gguaagggga uucaucagau cuucggcgcu     1920

gccuuuaaau cuuuguucgg aggcaugucg ugguuuagcc aaauccugau ugggacucuc     1980

cugguguggc uuggacugaa uaccaagaac ggcuccaucu cauugacgug ucucgcgcug     2040

gguggggugu uaauuuuccu gaguaccgcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8478
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 3 opt

<400>  8478
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcuguugagg ugacgagaag gggauccgca uacuauaugu accuggaccg gucugaugcc      120

gguaaggcga uuucauuccc caccacauug ggcguaaaca aaugcuauau acagaucaug      180

gacuuagggc acauguguga ugcuacuaug aguuacgaau gcccuaugcu ggacgaggga      240

guggaaccag augacgucga uuguuggugc aauaccacaa gcaccugggu gguuuauggc      300

acuugucauc acaagaaagg ugaggcccgc cgaucccgua gagcagugac acuaccgucu      360

cauucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguacacc      420

aaacaccuua uuagagucga gaacuggauc uuuaggaauc ccggguucgc ccuggcugca      480

gccgcuauug ccugguugcu cggaaguucc acaucucaga aggugaucua ucugguaaug      540

auacugcuca ucgcgccugc auacagcauu cggugcaucg gcguuuccaa ccgcgacuuu      600

guggaaggca ugucaggggg aacuuggguc gauguggugu uagagcaugg uggcuguguc      660

accguuaugg cccaggacaa gccaacagug gauauugaac ugguaaccac gacugugagu      720

aauauggcug agguccgaag cuacugcuau gaggccucua ucuccgacau ggcaucagau      780

agcagauguc ccacacaagg ggaggcuuac cuugacaaac agagugacac ccaguaugug      840

ugcaagagga cuuugguuga ucggggaugg ggcaacgguu gugggcuguu cggaaaaggc      900

ucucuaguga caugcgccaa guuugcgugc uccaaaaaga ugaccggcaa gagcauacag      960

ccugaaaauc ucgaauaccg uauuaugcug ucgguccacg ggucucaaca cuccggaaug     1020

aucgugaacg acauugguca ugagacggau gaaaaucgcg ccaaaguaga gaucacccca     1080

aacucaccca gagcagaggc uacacuuggc ggguucggaa gucugggcuu ggacugugaa     1140

ccuaggacug gucucgauuu uagcgaccug uauuacuuaa ccaugaauaa caagcacugg     1200

cugguucaua aagagugguu ccacgauauc ccacucccgu ggcacgccgg ggcugacaca     1260

ggcacucccc auuggaauaa caaggaagcc cuuguggagu uuaaagaugc acacgccaag     1320

cggcagaccg ucgugguccu gggaucccag gaaggcgcug ugcauacagc guuggcaggg     1380

gcccuagagg cugagaugga cggagccaaa ggucgacugu cuagcggcca ccucaagugc     1440

cgccugaaga uggauaaacu uagacugaag gggguuucau auaguuugug uaccgcagcc     1500

uucacuuuua cgaaaauucc ugcugaaaca cuccauggaa ccgugacugu agagguccag     1560

uacgccggca ccgacggucc augcaaggug cccgcacaaa uggcgguuga caugcagaca     1620

cugaccccug uggggagguu aauaacugcu aauccaguca ucacagaauc cacggagaac     1680

agcaaaauga ugcuggaacu agaucccccg uucggcgacu cuuacauugu gaucggagua     1740

ggcgauaaga agauaaccca ccacuggcau cggucaggga guacuauugg aaaagccuuu     1800

gaggcaacag ugcgcggugc caagagaaug gcuguucucg gcgacaccgc cugggauuuc     1860

gggagcgucg gaggcgcacu gaacucccuu gguaaaggga uccaccagau cuucggcgcu     1920

gccuuuaagu cuuuguucgg aggcaugucg ugguuuagcc aaauucugau cgggacacuc     1980

cugguguggc uuggacugaa uacuaaaaac ggcuccauuu cauugaccug ucucgcgcug     2040

gguggggugu uaauauuccu gaguacggcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8479
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 3 opt

<400>  8479
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua ucaagguuga gaacuggauu uuuagaaauc cgggguucac acugguggca      480

gucgccaucg cuugguugcu cggaaguucc acuucucaga aggugauaua ccugguaaug      540

auccugcuca uugcccccgc guauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aaccuggguc gauguggugu uagagcacgg uggcuguguc      660

acaguuaugg cacaggacaa accuaccgug gauaucgaac ugguaacgac uacagugagu      720

aauauggccg agguccgcag cuacugcuac gaggcuucua uauccgacau ggccucagau      780

agccgauguc caacccaagg ggaagcauau cuugacaagc agagugacac ucaguacgug      840

ugcaaaagaa cauugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga ccugcgcuaa auuuacgugc uccaagaaga ugaccggcaa aagcauucag      960

cccgagaauc ucgaauaucg gaucaugcug ucgguccacg ggucucaaca uuccggaaug     1020

auugugaacg acacagguca cgagacugau gaaaaucgug ccaagguaga gguuaccccu     1080

aacucaccac gcgcggaggc cacacuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacug gucucgauuu uagcgaccug uacuauuuaa ccaugaauaa caaacauugg     1200

cuggugcaca aggagugguu ccacgauauc ccucucccau ggcaugcagg ggcugacaca     1260

ggcaccccgc acuggaauaa caaagaagcc cuugucgagu uuaaggaugc ucaugccaaa     1320

aggcagacug uggucgugcu gggaucccag gaaggcgcag uucacacggc cuuggcuggg     1380

gcgcuagagg cagagaugga cggagccaag ggucggcugu ucucuggcca ucucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag ggggugagcu acucauugug uacagcugcc     1500

uuuaccuuca cuaagguacc cgcagaaacc cuccacggaa cagucaccgu ggagguucag     1560

uaugccggca cugacggucc uugcaaagug ccagcucaaa uggccgucga caugcagaca     1620

cugacgcccg uggggagauu aaucaccgca aauccuguaa uuacugaaag uacagagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauagu gaucggaguu     1740

ggcgauaaga aaauuaccca ccauuggcac aggucugggu caacaaucgg aaaggcguuc     1800

gaggcuacug uccggggugc caaacgcaug gcagugcucg gcgacaccgc cugggauuuc     1860

gggagugugg gaggcgucuu uaacagccug gguaagggga uacaucagau uuucggcgcu     1920

gccuuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaaucuugau cgggacgcug     1980

cucguuuggc ugggacuuaa uaccaagaac ggcagcauuu cccugacaug uuuggcacuc     2040

ggugggguga ugaucuuccu gucaacugcu gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8480
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 3 opt

<400>  8480
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccaacuug ggcgugaaua aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacaaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aacacuacca gcacaugggu aguguacggc      300

accugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac ucuaccaucu      360

cacucgacaa ggaagcucca aacccgguca cagacguggc uggaaagccg cgaguauacu      420

aaacaucuua uuaaggugga gaauuggauc uuuagaaacc cgggguucgc acuggccgcu      480

guugccauag cgugguugcu cggaaguucc accucucaga aggugaucua ccuggucaug      540

auucugcuca ucgcacccgc cuauagcauu aggugcaucg gcguguccaa ucgggacuuu      600

guagaaggca ugucaggggg aacauggguu gauguggucu uagagcacgg uggcugugug      660

acugugaugg cucaggacaa accuaccguc gauauagaac ugguuacaac cacggugagu      720

aacauggccg agguacgcag cuacugcuac gaggcaucua uuuccgacau ggcuucagau      780

agccgauguc caacucaagg ggaagccuau cuugacaagc agagugacac acaguacgug      840

ugcaaaagaa ccuuggucga uaggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

ucucuaguga cuugcgcgaa auuuacaugc uccaagaaga ugaccggcaa aagcauccag      960

cccgagaacc ucgaauaucg gauuaugcug ucgguucacg ggucucaaca uuccggaaug     1020

aucgugaaug acaucgguca cgagacggau gaaaaccgug ccaaggucga ggugaccccu     1080

aauucaccac gcgcagaggc uacacuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacug gucucgauuu uagcgaccug uacuauuuaa ccaugaacaa uaaacauugg     1200

cugguacaca aggagugguu ccacgauauu ccucucccau ggcaugccgg ggcugacaca     1260

ggcacuccgc acuggaacaa uaaagaagcc cuuguugagu uuaaggaugc acaugccaaa     1320

aggcagaccg uggucgugcu gggaucccag gaaggcgcug uccacacagc guuggcaggg     1380

gcccuagagg cugagaugga cggagccaag ggucggcugu ucucuggcca ucucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag ggggugagcu acucauugug uaccgcagcc     1500

uuuacuuuca cgaagguucc cgcugaaaca cuccacggaa ccgugacugu agagguccag     1560

uaugccggca ccgacggucc uugcaaagug ccagcacaaa uggcgguuga caugcagaca     1620

cugacccccg uggggagauu aauaacugcu aacccuguca ucacagaaag uacggagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu gaucggagua     1740

ggcgauaaga aaauaaccca ccauuggcac aggucugggu caacuauugg aaaggccuuc     1800

gaggcaacag ugcggggugc caaacgcaug gcuguucucg gcgacaccgc cugggauuuc     1860

gggagugucg gaggcguguu uaauagccug gguaaggggg ugcaucagau cuucggcgca     1920

gcuuuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaaucuugau ugggacacug     1980

cucgucuggc ugggacuuaa cacuaagaau ggcagcaucu cccugaccug uuuggcccuc     2040

ggugggguua ugauuuuccu gucaacggcg gugagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8481
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 3 opt

<400>  8481
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua uuaaggugga gaacuggauc uuuagaaauc cgggguucgc acugguugcc      480

guggcuauag ccugguugcu cggaaguucc acaucucaga aggucaucua ccuggugaug      540

auucugcuca ucgcgcccgc auauagcauu aggugcaucg gcguauccaa ccgggacuuu      600

guugaaggca ugucaggggg aacuugggug gaugucgugu uagagcacgg uggcugugug      660

accgucaugg cccaggacaa accuacaguu gauauagaac uggugaccac gacuguaagu      720

aauauggcug aggugcgcag cuacugcuac gaggccucua uuuccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacguc      840

ugcaaaagaa cuuuggugga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguua caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauccag      960

cccgaaaauc ucgaauaucg gauuaugcug ucggugcacg ggucucaaca uuccggaaug     1020

aucgucaacg acaucgguca cgagaccgau gaaaaucgug cgaaggugga gguaacaccu     1080

aacucaccac gcgccgaggc aacucuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaaccg gucucgauuu uagcgaccug uacuauuuaa caaugaauaa caaacauugg     1200

cugguucaca aggagugguu ccacgauauu ccucucccau ggcaugcugg ggccgacacu     1260

ggcaccccgc acuggaauaa caaagaagcu cuuguggagu uuaaggaugc ccaugcaaaa     1320

aggcagacag ucgugguccu gggaucccag gaaggcgccg ugcacaccgc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag ggucggcugu ucucuggcca ucucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag gggguuagcu acucauugug uacugccgca     1500

uuuacguuca caaaggugcc cgccgaaacc cuccacggaa cuguaaccgu cgaggugcag     1560

uaugcuggca cagacggucc uugcaaaguu ccagcccaaa uggcagugga caugcagacc     1620

cugacucccg ucgggagauu aauaacagcg aauccuguga ucacggaaag uaccgagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauugu aaucggagug     1740

ggcgauaaga aaauaacuca ccauuggcac aggucugggu caacaauugg aaaggcuuuc     1800

gaggccaccg uucggggugc aaaacgcaug gccguccucg gcgacacagc uugggauuuc     1860

gggagugugg gaggcguguu uaacagccug gguaagggga uccaucagau cuucggcgcc     1920

gcauuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaauuuugau cgggacucug     1980

cucgucuggc ugggacuuaa uaccaagaac ggcagcauuu cccugacgug uuuggcucuc     2040

ggugggguua ugauauuccu gucaaccgcc gugagugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8482
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 3 opt

<400>  8482
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccaacuug ggcgugaaua aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacaaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aacacuacca gcacaugggu aguguacggc      300

accugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugucac ucuaccaucu      360

cacucgacaa ggaagcucca aacccgguca cagacguggc uggaaagccg cgaguauacu      420

aaacaucuua uuaaggugga gaauuggauc uuuagaaacc cgggguucgc acuggccgcu      480

guugccauag cgugguugcu cggaaguucc accucucaga aggugaucua ccuggucaug      540

auucugcuca ucgcacccgc cuauagcauu aggugcaucg gcguguccaa ucgggacuuu      600

guagaaggca ugucaggggg aacauggguu gauguggucu uagagcacgg uggcugugug      660

acugugaugg cucaggacaa accuaccguc gauauagaac ugguuacaac cacggugagu      720

aacauggccg agguacgcag cuacugcuac gaggcaucua uuuccgacau ggcuucagau      780

agccgauguc caacucaagg ggaagccuau cuugacaagc agagugacac acaguacgug      840

ugcaaaagaa ccuuggucga uaggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

ucucuaguga cuugcgcgaa auuuacaugc uccaagaaga ugaccggcaa aagcauccag      960

cccgagaacc ucgaauaucg gauuaugcug ucgguucacg ggucucaaca uuccggaaug     1020

aucgugaaug acgagaaccg ugccaagguc gaagugacgc cuaauucacc acgcgcagag     1080

gcuacccuug guggcuucgg gagucuggga uuggauugug agcccagaac aggccucgac     1140

uuuagcgauc uguacuauuu aacuaugaac aauaaacacu ggcugguaca uaaggaaugg     1200

uuccacgaca ucccucuccc auggcacgcc ggugcugaua ccgggacacc gcauuggaac     1260

aauaaagagg cccuuguuga auuuaaggac gcacacgcca aaaggcagac uguggucgug     1320

cugggcuccc aggagggagc uguccauacc gcguuggcag gcgcccuaga agcugagaug     1380

gauggggcca agggacggcu guucucuggu caccucaagu gccgacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucauug uguacagcag ccuuuaccuu cacuaagguu     1500

cccgcugaga cgcuccaugg gacagugacc guagaagucc aguaugccgg aacugauggc     1560

ccuugcaaag ugccagcaca aauggcgguu gacaugcaga cccugacacc cguggguaga     1620

uuaauuaccg cuaacccugu cauaacugag aguacagaaa acuccaagau gaugcuggag     1680

cuagacccac ccuuugggga uagcuacauc gugauuggcg uaggagacaa gaaaaucacg     1740

caccacuggc auaggucugg cucaaccaua gggaaggccu ucgaagcaac ugugcgggga     1800

gccaaacgca uggcuguucu cggugauaca gccugggacu ucggcagugu cgggggagug     1860

uuuaauagcc ugggcaaggg uauucaccag aucuucgggg cagcuuuuaa aucccuuuuc     1920

ggcggaaugu cuugguuuuc gcaaaucuug auuggcaccc ugcucgugug gcuggggcuu     1980

aacacaaaga auggaagcau cucccugacu uguuuggccc ucggcggugu caugauuuuc     2040

cugucaaccg cgguuagugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8483
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 3 opt

<400>  8483
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua ucaagguuga gaacuggauu uuuagaaauc cgggguucgc acugguggcc      480

gucgcuaucg ccugguugcu cggaaguucc acaucucaga aggugauaua ccugguaaug      540

auccugcuca uugcgcccgc auauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aacuuggguc gauguggugu uagagcacgg uggcuguguc      660

accguuaugg cccaggacaa accuacagug gauaucgaac ugguaaccac gacugugagu      720

aauauggcug agguccgcag cuacugcuac gaggccucua uauccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacgug      840

ugcaaaagaa cuuugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauucag      960

cccgaaaauc ucgaauaucg gaucaugcug ucgguccacg ggucucaaca uuccggaaug     1020

auugugaacg acaccgguca cgagacagau gaaaaucgug cgaagguaga gguuacuccu     1080

aacucaccac gcgccgaggc aacccuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaacag gucucgauuu uagcgaccug uacuauuuaa cuaugaauaa caaacauugg     1200

cuggugcaca aggagugguu ccacgauauc ccucucccau ggcaugcugg ggccgacacc     1260

ggcacaccgc acuggaauaa caaagaagcu cuugucgagu uuaaggaugc ccaugcaaaa     1320

aggcagaccg uggucgugcu gggaucccag gaaggcgccg uucacacugc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag ggucggcugu ucucuggcca ucucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag ggggugagcu acucauugug uacggccgca     1500

uuuacauuca ccaagguacc cgccgaaacu cuccacggaa ccgucacagu ggagguucag     1560

uaugcuggca ccgacggucc uugcaaagug ccagcccaaa uggcagucga caugcagacu     1620

cugacacccg uggggagauu aaucacggcg aauccuguaa uuaccgaaag uacugagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacauagu gaucggaguu     1740

ggcgauaaga aaauuacaca ccauuggcac aggucugggu caaccaucgg aaaggcuuuc     1800

gaggccacag uccggggugc aaaacgcaug gccgugcucg gcgacacugc uugggauuuc     1860

gggagugugg gaggcgucuu uaacagccug gguaagggga uacaucagau uuucggcgcc     1920

gcauuuaaau cccuuuucgg aggcaugucu ugguuuucgc aaaucuugau cgggacccug     1980

cucguuuggc ugggacuuaa uacgaagaac ggcagcauuu cccugaccug uuuggcucuc     2040

ggugggguga ugaucuuccu gucaacagcc gugagugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8484
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 3 opt

<400>  8484
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucaac acaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cauucgacca ggaagcucca gacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaccuua ucaagguuga gaacuggauu uuuagaaauc cgggguucgc acugguggcc      480

gucgcuaucg ccugguugcu cggaaguucc acaucucaga aggugauaua ccugguaaug      540

auccugcuca uugcgcccgc auauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aacuuggguc gauguggugu uagagcaugg uggcuguguc      660

accguuaugg cccaagacaa accuacagug gauaucgaac ugguaaccac gacugugagu      720

aauauggcug agguccgcag cuacugcuac gaggccucua uauccgacau ggcaucagau      780

agccgauguc caacacaggg ggaggcuuau cuugacaagc agagugacac ccaguacgug      840

ugcaaaagaa cuuugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauucaa      960

cccgaaaauc ucgaauaucg gaucaugcug ucgguccacg ggucucagca cuccggaaug     1020

auugugaacg acaucgguca ugagaccgau gaaaaucgug cgaagguaga gguuacaccu     1080

aacucaccac gcgccgaggc aacucuuggc ggguucggaa gucugggcuu ggacugugaa     1140

cccagaaccg gucucgauuu uagcgaccug uacuauuuaa caaugaauaa caaacacugg     1200

cuggugcaua aggagugguu ccacgauauc ccucucccau ggcacgcugg ggccgacacu     1260

ggcaccccgc auuggaauaa caaagaagcu cuugucgagu uuaaggaugc ccacgcaaaa     1320

aggcagacag uggucgugcu gggaucccag gaaggcgccg uucauaccgc uuuggcgggg     1380

gcacuagagg ccgagaugga cggagcuaag ggucggcugu ucucuggcca ccucaagugc     1440

cgacugaaaa uggauaaacu ucgccugaag ggggugagcu acucauugug uacugccgua     1500

ugcacggcag ccaagguccc cgcugaaaca cuccauggaa ccgugacugu ugaggugcaa     1560

uaugccggca ccgacggucc uuguaaaguc ccagcacaga uggcggugga caugcagaca     1620

cugacccccg uagggagauu aauuacugcu aauccuguga uaacagaaag uacggagaac     1680

uccaagauga ugcuggaacu agauccaccc uuuggcgaca gcuacaucgu uauuggaguc     1740

ggcgauaaga aaaucaccca ccacuggcau aggucugggu caacuauagg aaaggccuuc     1800

gaggcaacag ugcggggugc caaacgcaug gcugugcucg gcgacaccgc cugggauuuu     1860

gggagugucg gaggcguuuu caacagccug gguaagggga uucaccaaau cuucggcgca     1920

gcuuuuaaau cccuuuucgg aggcaugucu ugguuuucgc agaucuugau ugggacacug     1980

cucguguggc ugggacuuaa uacuaagaac ggcagcaucu cccugaccug cuuggcccuc     2040

ggugggguga ugauuuuccu gucaacggcg guaagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8485
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 3 opt

<400>  8485
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccuuac aacugccaug       60

gcugcagaga uaacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua ucucauucgc caccacauug ggcgucaaca aaugccacgu ucagauuaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guggaaccug augacguaga uuguuggugc aauaccacaa gcaccugggu ggucuacggc      300

acuugucacc auaagaaagg ugaggcccgc cgaucccgua gagcugugac acuaccaucu      360

cacucgacca ggaagcucca aacgcgguca cagacuuggc uggaaagccg cgaguauacc      420

aaacaucuua ucaagguuga gaacuggauu uuuagaaauc cgggguucgc acugguggcc      480

gucgcuaucg ccugguugcu cggaaguucc acaucucaga aggugauaua ccugguaaug      540

auccugcuca uugcgcccgc auauagcauc aggugcauug gcguuuccaa ccgggacuuu      600

guggaaggca ugucaggggg aacuuggguc gauguggugu uagagcacgg uggcuguguc      660

accguuaugg cccaggacaa accuacagug gauaucgaac ugguaaccac gacugugagu      720

aauauggcug agguccgcag cuacugcuac gaggccucua uauccgacau ggcaucagau      780

agccgauguc caacacaagg ggaggcuuau cuugacaagc agagugacac ccaguacgug      840

ugcaaaagaa cuuugguuga uaggggaugg ggcaacgguu gugggcuguu cggaaagggc      900

ucucuaguga caugcgccaa auuuaccugc uccaagaaga ugacgggcaa aagcauucag      960

cccgaaaauc ucgaauaucg gaucaugcug ucgguccacg ggucucaaca uuccggaaug     1020

auugugaacg acgagaaucg ugcgaaggua gaaguuaccc cuaacucacc acgcgccgag     1080

gcaacacuug guggcuucgg gagucuggga uuggauugug agcccagaac uggccucgac     1140

uuuagcgauc uguacuauuu aaccaugaau aacaaacacu ggcuggugca uaaggaaugg     1200

uuccacgaca ucccucuccc auggcacgcu ggugccgaua cagggacucc gcauuggaau     1260

aacaaagagg cucuugucga auuuaaggac gcccacgcaa aaaggcagac cguggucgug     1320

cugggcuccc aggagggagc cguucauaca gcuuuggcgg gcgcacuaga agccgagaug     1380

gauggggcua agggacggcu guucucuggu caccucaagu gccgacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucauug uguaccgccg cauuuacuuu cacgaaggua     1500

cccgccgaga cacuccaugg gaccgucacu guggaaguuc aguaugcugg aaccgauggc     1560

ccuugcaaag ugccagccca aauggcaguc gacaugcaga cacugacccc cguggguaga     1620

uuaaucacug cgaauccugu aauuacagag aguacggaaa acuccaagau gaugcuggag     1680

cuagacccac ccuuugggga uagcuacaua gugaucggcg uuggagacaa gaaaauuacc     1740

caccacuggc auaggucugg cucaacuauc gggaaggcuu ucgaagccac aguccgggga     1800

gcaaaacgca uggccgugcu cggugauacc gcuugggacu ucggcagugu cgggggagug     1860

uuuaacagcc ugggcaaggg uauacaccag auuuucgggg ccgcauuuaa aucccuuuuc     1920

ggcggaaugu cuugguuuuc gcaaaucuug aucggcacac ugcucguuug gcuggggcuu     1980

aauacuaaga acggaagcau uucccugacc uguuuggcuc ucggcggugu gaugaucuuc     2040

cugucaacgg ccgugagugc gugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8486
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 3 opt

<400>  8486
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcagaga ucacgagaag gggauccgcc uacuauaugu accuggaccg gucugaugcg      120

gguaaggcua uuucauucgc caccacauug ggcgugaaca aaugccacgu ccagaucaug      180

gacuuagggc auauguguga ugcaacuaug aguuaugaau gccccaugcu ggacgaggga      240

guugaaccug augacgugga uuguuggugc aauaccacaa gcaccugggu aguguacggc      300

acuugucacc auaagaaagg ugagacacgc cgaucccgua gaucugucuc gcuaagguau      360

cacuacaccc ggaagcucca aacgcgcuca cagacuuggc uggaaagcag agaguauaaa      420

aagcaucuua uuauggugga gaacuggauc uuuaggaauc caggguucgc cauaguuagu      480

guggcuauca ccuggcugau gggauccuug acaucucaga aggucauuua ccucgugaug      540

aucguacuga uuguuccggc auacagcauc uccugcauag gcgugucaaa ccgggaccug      600

gucgaaggca ugaguggggg aacuugggug gauguggucc ucgagcacgg uggcuguguu      660

accgaaaugg cccaggacaa acccacagug gauauugagu uaguaaccau gacggugagc      720

aauauggcug agguccgcuc uuauugcuac gaagccuccc ugucagacau ggcgagcgca      780

agucgauguc cuacucaagg ggagccaucu cuugauaagc aguccgacac acagagcgug      840

ugcaaaagaa ccuugggaga caggggcugg gguaacgggu guggaaucuu uggcaagggc      900

ucgcugguua cuugcucuaa auucacaugc uguaagaaga ugcccgggaa auccauucag      960

ccugaaaauc uagaguaucg gaucaugcuc ccagugcacg gaucacaaca uagugguaug     1020

aucgucaacg auauuggcca cgaaaccgac gagaaucgug ccaaggugga gguaacgccc     1080

aacagcccuc gcgcugaagc cacccuggga ggguuuggcu cccuuggucu ggauugcgag     1140

ccaagaacag gguuggacuu cucugaucuc uacuaucuga cuaugaauaa caaacauugg     1200

uuaguucaca aggaaugguu ucacgacaua ccgcugcccu ggcaugcagg cgcugauacc     1260

ggaacaccuc acuggaauaa caaagaggcc cucguggagu ucaaggacgc gcaugccaaa     1320

aggcagacug ucgugguccu uggcagccag gaaggggcag ugcacaccgc ucuggccgga     1380

gcuuuggagg ccgaaaugga uggugcaaag ggccggcuau uuucagggca ucugaagugu     1440

cgacucaaaa uggacaagcu gcgccuuaaa ggaguuaguu acucccugug cacagccgcu     1500

uucaccuuua cuaaggugcc agcggagacg uugcacggca caguaaccgu cgaagugcag     1560

agcgcaggua cugaugggcc cuguaaaguu ccugcccaaa uggcugugga caugcagacc     1620

cucacaccag ucggcagacu gaucaccgcc aaucccguga uuacugaguc uacagaaaac     1680

ucaaagauga uguuagagcu ggacccgccu uucggagaua guuauaucgu aauaggcgug     1740

ggggacaaga aaauuacgca ccauuggcac aggagcggau ccaccaucgg uaaggcauuu     1800

gaggccacug uucggggcgc uaaacgcaug gccguccuag gggauacagc augggacuuc     1860

ggaucugugg gcgguguguu caacucgcuc gggaagggca uccaucagau uuuuggagcg     1920

gcuuucaaaa gccuguuugg cgggaugucc ugguucucac aaauccuuau uggaaccuug     1980

cuggucuggc ucggccugaa uacaaagaac gguaguauau cucuuacuug ccuggcccuc     2040

gggggaguua ugaucuuuuu gagcaccgca guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8487
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 3 opt

<400>  8487
gggagaaagc uuaccaugag cgugggcauc gucgggcugc uccuuaccac agccauggcu       60

gcagagguua cuagaagggg auccgccuac uauauguacc uggaccggaa cgaugcgggu      120

gaagcuauuu cuuuccccac gaccuugggc augaauaagu gcuauauaca gaucauggac      180

uuagggcaca ugugugaugc cacaauguca uacgagugcc cuaugcugga cgaaggagug      240

gagccagaug acguagauug uuggugcaac acuaccagua cauggguggu cuauggcacc      300

ugucaucaca aaaaggguga agcacgccga agccguagag ccgugacucu accgucccau      360

ucuacaagga aacuccaaac ccggucgcag acguggcugg agucacgcga guacacuaag      420

caccuuauua gaguugaaaa uuggaucuuu aggaaccccg gguucgcucu ggcagccgcu      480

gccauugcgu gguugcucgg aagcaguacc ucccagaaag ugaucuaucu ggucaugaua      540

cugcucaucg ccccugcaua cucuauucgg ugcaucggcg ugagcaaucg cgacuuugua      600

gagggcaugu ccgggggaac auggguugau guggucuuag aacauggugg cugugugacu      660

gugauggcuc aggacaagcc aaccgucgau auugagcugg uuacaaccac ggugucaaac      720

auggccgagg uacgaaguua cugcuaugaa gcaagcaucu cugacauggc uuccgauuca      780

agauguccca cucaagggga ggccuaccuu gacaagcaga gcgacacaca guaugugugc      840

aaaaggaccu uggucgaucg gggauggggc aaugguugug ggcuguucgg aaagggcagu      900

cuagugacuu gcgcgaaauu ugccugcucu aagaaaauga caggcaaguc cauacagccu      960

gaaaaccucg aguaccguau uaugcugagc guucacgggu cgcaacacuc uggaaugauc     1020

gugaaugaca ccggucauga aacggaugag aaccgcgcaa aggucgagau uaccccaaau     1080

ucccccagag cugaagccac acuuggcggg uucggaucac ugggcuugga cugugagccu     1140

aggacugguc ucgauuuuag ugaccuguau uacuuaacca ugaacaauaa acacuggcug     1200

gugcauaagg aaugguucca cgauauccca cucccguggc acgcuggggc cgacacaggc     1260

acuccccauu ggaacaauaa agaggcacuu guagaauuua aggaugccca cgcuaaacgg     1320

cagaccguug ugguccuggg aagccaggag ggcgcggugc auacagcauu ggccggggcu     1380

cuagaggccg aaauggacgg agcaaagggu cgacuguccu cuggccaccu caaaugccgc     1440

cugaagaugg auaaacuuag acugaagggg gucagcuauu cauuguguac cgccgcuuuc     1500

acuuuuacga agaucccugc cgagacacuc cauggaaccg ugacuguuga agugcaguac     1560

gcaggcaccg acgguccaug caaaguaccc gcgcaaaugg cugucgacau gcagacacug     1620

accccugugg ggagguuaau uacugccaac ccaguuauaa cagagaguac ggaaaacucc     1680

aagaugaugc uggagcuaga ucccccguuc ggcgacagcu acaucgugau uggagucggc     1740

gagaaaaaga ucacccacca cuggcaucgg ucugggucaa cuauaggaaa ggcauuugaa     1800

gccacagugc gcggugcuaa aagaauggcc guacucggcg auaccgcaug ggacuucggg     1860

agugugggag gcgcucugaa uagccuuggu aaggggauuc accagaucuu cggcgccgcg     1920

uuuaaauccu uguucggagg caugucuugg uuuucgcaaa uccugauugg gacacuccug     1980

auguggcuug gacugaacac uaagaauggc agcaucuccu ugaugugucu cgcccugggu     2040

gggguuuuaa uuuuccuguc aaccgcuguc agugcaugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  8488
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 3 opt

<400>  8488
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauauugagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gauuacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aauuacagcc aacccaguua uaacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucacuca ccacuggcau cggucugggu caacaauagg aaaggcauuu     1800

gaagccaccg ugcgcggugc uagaaggaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaaaggga uucaccagau cuucggcgcc     1920

gcguuuaagu ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacucuc     1980

cugauguggc uuggacugaa caccaaaaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8489
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 3 opt

<400>  8489
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggaug uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccaucg cgugguugcu cggaagcagu acaucccaga aagugauuua ucuggucaug      540

auccugcuca uagcacccgc cuacucuauc cggugcauug gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgugaugg cucaggacaa gccaacaguc gauaucgagc ugguuaccac gacuguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca uuucugacau ggcuuccgau      780

ucaagauguc ccacacaagg ggaggccuac cuugacaagc agagcgacac ccaguaugug      840

ugcaaaagga cuuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga caugcgcgaa auuugccugc ucuaagaaaa ugaccggcaa guccauccag      960

ccugaaaacc ucgaguaccg uauaaugcug agcguucacg ggucgcaaca cucuggaaug     1020

auugugaaug acacggguca ugaaaccgau gagaaccgcg caaaggucga gaucacacca     1080

aauuccccca gagcugaagc cacucuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggaccg gucucgauuu uagugaccug uauuacuuaa caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauu ccacucccgu ggcacgcugg ggccgacacu     1260

ggcaccccuc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagacag uugugguccu gggaagccag gagggcgcgg ugcauaccgc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uacugccgcu     1500

uucacguuua caaagauccc ugccgagacc cuccauggaa cugugaccgu ugaagugcag     1560

uacgcaggca cagacggucc augcaaagua cccgcgcaaa uggcugucga caugcagacc     1620

cugacuccug uggggagguu aaucacagcc aacccaguua uuacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauagu gaucggaguc     1740

ggcgagaaaa agauuacuca ccacuggcau cggucugggu caacaaucgg aaaggcauuu     1800

gaagccaccg ugcgcggugc uaaaagaaug gccguacucg gcgauacagc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uacaccagau uuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau cgggacucuc     1980

cugauguggc uuggacugaa caccaagaau ggcagcauuu ccuugaugug ucucgcccug     2040

ggugggguuu uaaucuuccu gucaacggcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8490
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 3 opt

<400>  8490
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaugacca guacaugggu ggucuauggc      300

accugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac ucuaccgucc      360

cauucuacaa ggaaacucca aacccggucg cagacguggc uggagucacg cgaguacacu      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu accucccaga aagugaucua ucuggucaug      540

auacugcuca ucgccccugc auacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacauggguu gauguggucu uagaacaugg uggcugugug      660

acugugaugg cucaggacaa gccaaccguc gauauugagc ugguuacaac cacgguguca      720

aacauggccg agguacgaag uuacugcuau gaagcaagca ucucugacau ggcuuccgau      780

ucaagauguc ccacucaagg ggaggccuac cuugacaagc agagcgacac acaguaugug      840

ugcaaaagga ccuuggucga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguga cuugcgcgaa auuugccugc ucuaagaaaa ugacaggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcguucacg ggucgcaaca cucuggaaug     1020

aucgugaaug acaccgguca ugaaacggau gagaaccgcg caaaggucga gauuacccca     1080

aauuccccca gagcugaagc cacacuuggc ggguucggau cacugggcuu ggacugugag     1140

ccuaggacug gucucgauuu uagugaccug uauuacuuaa ccaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccacucccgu ggcacgcugg ggccgacaca     1260

ggcacucccc auuggaacaa uaaagaggca cuuguagaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg uugugguccu gggaagccag gagggcgcgg ugcauacagc auuggccggg     1380

gcucuagagg ccgaaaugga cggagcaaag ggucgacugu ccucuggcca ccucaaaugc     1440

cgccugaaga uggauaaacu uagacugaag ggggucagcu auucauugug uaccgccgcu     1500

uucacuuuua cgaagauccc ugccgagaca cuccauggaa ccgugacugu ugaagugcag     1560

uacgcaggca ccgacggucc augcaaagua cccgcgcaaa uggcugucga caugcagaca     1620

cugaccccug uggggagguu aauuacugcc aacccaguua uaacagagag uacggaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacaucgu gauuggaguc     1740

ggcgagaaaa agaucaccca ccacuggcau cggucugggu caacuauagg aaaggcauuu     1800

gaagccacag ugcgcggugc uaaaagaaug gccguacucg gcgauaccgc augggacuuc     1860

gggagugugg gaggcgcucu gaauagccuu gguaagggga uucaccagau cuucggcgcc     1920

gcguuuaaau ccuuguucgg aggcaugucu ugguuuucgc aaauccugau ugggacacuc     1980

cugauguggc uuggacugaa cacuaagaau ggcagcaucu ccuugaugug ucucgcccug     2040

ggugggguuu uaauuuuccu gucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8491
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 3 opt

<400>  8491
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcagagg uuacgagaag gggauccgcc uacuauaugu accuggaccg gaacgaugcg      120

ggugaagcua uuucuuuccc caccacauug ggcaugaaua agugcuauau acagaucaug      180

gacuuagggc acauguguga ugccacuaug ucauacgagu gcccuaugcu ggacgaagga      240

guggagccag augacguaga uuguuggugc aacaccacaa guaccugggu ggucuauggc      300

acuugucauc acaaaaaggg ugaagcacgc cgaagccgua gagccgugac acuaccgucc      360

cauucuacca ggaaacucca aacgcggucg cagacuuggc uggagucacg cgaguacacc      420

aagcaccuua uuagaguuga aaauuggauc uuuaggaacc ccggguucgc ucuggcagcc      480

gcugccauug cgugguugcu cggaagcagu acaucccaga aagugaucua ucuggucaug      540

auacugcuca ucgcacccgc cuacucuauu cggugcaucg gcgugagcaa ucgcgacuuu      600

guagagggca uguccggggg aacuuggguu gauguggucu uagaacaugg uggcugugug      660

accgcuaugg cccaggacaa gccaacagug gauauugagc uggucaccac gacuguuuca      720

aacauggcag aggugcgaag uuacugcuau gaagcuagca ucucugacau ggccuccgau      780

ucaagauguc ccacacaagg ggaggcguac cuugacaagc agagcgacac ccaguaugua      840

ugcaaaagga cuuuggugga ucggggaugg ggcaaugguu gugggcuguu cggaaagggc      900

agucuaguca caugcgccaa auuugcaugc ucuaagaaaa ugaccggcaa guccauacag      960

ccugaaaacc ucgaguaccg uauuaugcug agcgugcacg ggucgcaaca cucuggaaug     1020

cuuguuaaug acacggguca ugaaaccgau gagaaccgcg cuaaggugga gaucacacca     1080

aauuccccca gagccgaagc uacucugggc ggguucggau cauugggccu cgacugugag     1140

ccuaggaccg gucuggauuu uagugacuua uauuaccuga caaugaacaa uaaacacugg     1200

cucgcccaua aggaaugguu ccacgauauu ccacuuccgu ggcacgcagg ggccgcuacu     1260

ggcaccccuc auuggaacaa uaaagaggcg cuggucgaau uuaaggacgc acacgccaaa     1320

cggcagacag ugguaguuuu gggaagccag gagggcgcug ugcauaccgc ccuagcaggg     1380

gcccuggagg cugaaaugga uggagccaag ggucgacucu ccucuggcca ccugaaaugc     1440

cgccuuaaga uggacaagcu gagauugaaa ggggucagcu auucacucug uacugcagcg     1500

uucacguuua caaagauccc ugcugagacc guggauggaa cugucaccgu ggaaggccag     1560

uacgguggga cagacggccc augcaaaguu cccgcccaaa uggcagugga caugcagacc     1620

cugacuccug uaggaagguu aaucacagcc aacccaguca uuacggagag uaccgaaaac     1680

uccaagauga ugcuggagcu agaucccccg uucggcgaca gcuacauagu gaucgggguu     1740

ggagagaaaa agauuacuca ucacuggcac cggucugguu caacaaucgg caaggcuuuu     1800

gaagccaccg ugcgcggggc aaaaagaaug gcuguccucg gagauacagc cugggacuuc     1860

ggcagugugg guggggcgcu gaauagccuu ggcaagggaa uacaucagau uaucggcgcc     1920

gcuuucaaau ccuuguuugg gggaaugucu ugguucucgc aaauccugau uggcacucuc     1980

cugauguggc uuggucugaa caccaagaau gggagcaucu ccuugaugug ucucgcacug     2040

ggaggcguau uaauuuuucu gucaacggcc gugagugguu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8492
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 3 opt

<400>  8492
gggagaaagc uuaccauggg cgccgacgcu agcaucggga uugugucccu gcuccuuacc       60

acagucaugg cagccgagau aacuagaagg ggaucugcgu acuauaugua ccuggaucgg      120

ucagacgcug guaaggccau caguuucguu acgaccuugg gcgugaacaa augccacgua      180

cagauuaugg auuuagggca uaugugugac gcaacaauga gcuaugaaug ccccaugcug      240

gaugagggag uggaaccuga cgaugucgac uguuggugca auacuaccuc cacaugggug      300

guuuacggca ccugucacca uaagaaaggu gaggcccgcc gaucucguag agcugugacu      360

cuaccaucgc acucaacaag gaagcuccaa acccggagcc agacguggcu ggaaagucgc      420

gaguauacua aacaucuuau caaggucgag aacuggauuu uuagaaaucc gggguucgca      480

cugguggccg uagcuaucgc cugguugcuc ggauccucua ccagccagaa gguuauauac      540

cuggugauga uccugcucau ugcgcccgca uauuccauca ggugcauugg cgucucaaac      600

cgggauuuug uggaaggcau gaguggggga acaugggugg acgucguuuu agagcacggu      660

ggcuguguga cuguaauggc ccaggauaaa ccuaccgugg acaucgaacu ggucacaacc      720

acggugagca auauggcuga gguucgcucu uacugcuacg aggccuccau aucagauaug      780

gcaagcgaca gucgaugucc aacucaaggg gaggcuuauc uugacaagca gucugauaca      840

caguacgugu gcaaaagaac cuuggucgac aggggauggg gcaacgguug ugggcuguuc      900

ggaaagggcu cccuagugac uugcgccaaa uuuacaugca gcaagaagau gaccggcaaa      960

ucgauucagc ccgaaaaucu cgaauaucgg aucaugcugu cuguacacgg gucccaacau     1020

ucaggaauga uuguuaacga ugagaaucgu gcgaaggugg aagucacgcc uaacagucca     1080

cgcgccgagg caacccuugg uggcuucggg agccugggau uggacuguga gcccagaaca     1140

ggccucgauu uuuccgaccu guacuauuua acuaugaaua acaaacacug gcuggugcau     1200

aaggaauggu uccacgauau cccucuccca uggcacucug gugcugacac cgagacaccg     1260

cauuggaaua acaaagaagc ccuugucgag uuuaaggaug cucacgccaa aaggcagacu     1320

gugguugugc uggggagcca ggaaggcgca guacauaccg ccuuggcugg agcgcuagag     1380

gcagagaugg acggcgccaa ggggcggcug ucaaguggac accucaagug ccgacugaaa     1440

auggauaaac uucgccugaa gggugucucc uacagcuugu guacagcugc cuucaccuuu     1500

acuaaggugc ccgcagaaac gcuccauggc acaguuaccg uggaggucca guaugccggg     1560

agagacggac cuugcaaagu gccagcucaa auggccguag acaugcagac ucugaccccc     1620

gugggcaggu uaaucacagc aaauccuguu auuaccgaau cuacugagaa cucaaagaug     1680

augcuggaac uagauccacc cuucggugac aguuacauag ucaucggggu gggcgauaag     1740

aaaauuacac accacuggca ucggagcgga uccaucauag gcaaggcguu ugaggcuacg     1800

gugcgcgggg ccaaaagaau ggcaguccuc ggagacaccg ccugggauuu cgguucuguu     1860

ggcggggugu ucaacucgcu gggaaagggc auucaccaga ucuuuggugc ugccuucaaa     1920

agccuuuuug ggggcauguc cugguucuca caaaucuuga uuggaacucu gcucgugugg     1980

cugggccuua auacaaagaa cgggaguauc ucucugaccu guuuggcacu cggaggcgua     2040

augauuuuuc ugagcacagc ugucuccgcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  8493
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 3 opt

<400>  8493
gggagaaagc uuaccauggg cgccgacacc agcaucggga uuauaggacu gcuccuuaca       60

acugcuaugg cagccgagau cacgagaagg gguuccgcgu acuauaugua ccuggaucgg      120

ucugacgcug gcaaggccau uucauucgca accacaaugg gggugaacaa augccacguc      180

cagaucaugg auuugggaca uaugugugac gccacuauga guuaugaaug ccccauguua      240

gaugagggcg uugaaccuga cgauguggac uguuggugca auaccacaag caccugggua      300

guguacggua cuugucacca uaagaaaggg gaggcucgcc caucccgacg ugcagucaca      360

cugccgucuc acucgaccag aaagcuacaa acgaggucac agacuuggcu cgaaagccgg      420

gaguauacca aacaucugau uaagguggag aacuggaucc uucgcaaucc cggauuuaca      480

guugccgcuc uggccauggc gugguugcuc ggcaacagua cuucccagag agugauauac      540

cuggucauga uccugcucau ugcacccgcc uauucuauca ggugcauugg cgugagcaau      600

cgggauuucg uagaagggau guccggaggu accuggguug acguggucuu agagcacggc      660

ggguguguga cagugauggc ucaggauaag ccaaccgucg acaucgaacu gguuacgacu      720

acagugucaa acauggccga gguacgcagu uacugcuacg aggcaagcau aucugauaug      780

gcuuccgacu cacgaugucc cacccaagga gaagccuauc uugacaaaca gagcgauacu      840

caguacgugu gcaagagaac auuggucgac aggggcuggg guaaugggug uggacuguuu      900

ggcaaaggca gucuagugac cugcgcgaag uucacgugcu cuaaaaagau gaccgggaag      960

uccauucagc cugagaaccu cgaauaucgg aucaugcuga gcguucacgg aucgcaacau     1020

ucugguauga uugugaauga uaucggccac gagacagacg aaaaccgugc caaagucgag     1080

gugacuccaa auuccccucg cgcagaggcu acccuugggg gauuuggcuc acuggguuug     1140

gauugugaac cuagaacagg gcucgacuuc agugaucugu acuauuuaac uaugaacaau     1200

aagcauuggc ugguacacaa agagugguuu cacgacaucc cacucccgug gcaugccggc     1260

gcugauaccg gaacacccca cuggaacaau aaggaagccc uuguugaguu caaagacgca     1320

caugccaaga ggcagaccgu ggucgugcug ggcagccagg aaggggcugu ccacacugcg     1380

uuggcaggag cccuagaggc ugagauggau ggugccaaag gccggcuguu uuccgggcau     1440

cucaagugcc gaauuaagau ggacaaacug cgccuuaagg gagugucuua cagccugugu     1500

acggcagccu ucacauuuuc aaaaguuccu gcugaaaccu ugcacggcac ugugaccgua     1560

gagguccagu augccgguag agacgggcca ugcaaggugc ccgcacaaau ggcgguugau     1620

augcagacac ucaccccugu gggcaggcug auaacugcua acccaaucau uacagaaagu     1680

acggagaacu cccggaugau guuagaacug gaucccccgu ucggagacag cuacaucguc     1740

auaggcgugg gggacaaaaa gauuacccac cauuggcacc gcucuggauc aacuaucggu     1800

aaggccuuug aggcaacagu aagaggcgcc aaaaggaugg cugugcuagg ggauaccgcc     1860

ugggacuucg gaaguguugg cggugucuuc aauagccucg ggaagggcgu gcaucaggug     1920

uuuggagcag cuuucaaauc ccuguuuggc gggaugucuu gguucucgca aauccuuauu     1980

ggaacauugc uggucuggcu cggccugaac acuaagaaug guagcaucuc ccuugccugu     2040

cuggcguugg gaggggccau gauuuuucuc ucaaccgcug uuagugcaug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8494
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 3 opt

<400>  8494
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagauau cgugcuggaa cacggcgggu gugucacagu gauggcacaa      180

gacaaaccua cuguugauau ugaguuggug acgaccacag ucaguaauau ggccgaagug      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag guggaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ccauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ugaguucaag gaugcacacg ccaagagaca gacaguggua      840

guguugggcu cccaggaagg ggcuguccau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auccccgcug agacccugca cggcacuguu acaguggaag uccaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacguuaac cccgguuggc     1140

cgacugauaa cagcaaaucc cgugauuacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgucauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8495
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 3 opt

<400>  8495
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8496
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 3 opt

<400>  8496
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgg cuaugaaacc      540

gaugaggaca gggccaaggu ggaagucacg cccaacaguc cucgggcuga ggcaacucug      600

ggaggguuug gcagcuuggg ucucgauugc gagccacgca ccgggcugga cuucucugau      660

cuguacuacc ucacaaugaa uaacaaacau ugguuaguuc acaaggaaug guuucaugac      720

auuccccugc cuuggcacgc cggagcugac acuggcaccc cacacuggaa uaacaaagag      780

gcccuugugg aguucaagga ugcgcaugca aagagacaga caguaguggu cuugggcucc      840

caggaagggg ccgugcacac cgcucuggcc ggagcacuag aagcugagau ggacggugcc      900

aaaggcaggc ucuuuucagg gcaucugaag ugucggcuua aaauggauaa gcugcgcuug      960

aaaggaguua gcuauagucu cugcacggcg gccuucacuu uuacaaaggu gcccgcagag     1020

acccugcacg gcacugucac aguggaagua caauacgcug guaccgacgg gccuuguaaa     1080

aucccaguuc agauggccgu ggauaugcag acguuaaccc cggucggccg acugauuaca     1140

gcuaaucccg ugaucacuga gucuaccgaa aacuccaaga ugaugcucga gcuugacccu     1200

ccauucggag auagcuauau agucauuggc gugggggaca agaaaaucac acaccauugg     1260

cacagaucgg gaucuacuau ugguaaggcc uuugaagcaa ccguuagggg cgccaaacgg     1320

auggcugugc ugggggauac agcgugggac uucggauccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8497
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 3 opt

<400>  8497
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8498
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 3 opt

<400>  8498
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauacc      540

ggcuaugaaa cggacgagaa uagggccaag gucgaaguua cucccaacag uccucgggcu      600

gaggcaaccc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacagggcug      660

gacuucucug aucuguacua ccucacuaug aauaacaaac auugguuagu gcacaaggaa      720

ugguuucaug acauuccccu gccuuggcac gccggagcug acaccggcac accacacugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcaug caaagagaca gaccgugguc      840

guguugggcu cccaggaagg ggccguucac acggcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcaa gcucuuuuca gggcaucuga aauguaggcu uaagauggau      960

aaacugcggu ugaagggagu gagcuauagu cucugcacug cggccuucac auuuaccaaa     1020

guccccgcag agacucugca cggcacagug accguagaag uucaauacgc ugguacggac     1080

gggccuugua agaucccagu gcagauggcc gucgauaugc agaccuuaac accggugggc     1140

cgccugauua cugcuaaucc cgucaucacc gagucuacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuau auagugauug gcguugggga caaaaagauc     1260

acucaccauu ggcaccgauc gggaucuacc auugguaaag ccuuugaagc aacagugaga     1320

ggcgccaaga ggauggcugu acugggggau accgcguggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8499
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 3 opt

<400>  8499
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaccgu gaacgauauc      540

ggcuaugaaa cggacgagaa uagggccaag gucgaaguua cucccaacag uccucgggcu      600

gaggcaaccc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacagggcug      660

gacuucucug aucuguacua ccucacuaug aauaacaaac auugguuagu gcacaaggaa      720

ugguuucaug acauuccccu gccuuggcac gccggagcug acaccggcac accacacugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcaug caaagagaca gaccgugguc      840

guguugggcu cccaggaagg ggccguucac acggcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcaa gcucuuuuca gggcaucuga aauguaggcu uaagauggau      960

aaacugcggu ugaagggagu gagcuauagu cucugcacug cggccuucac auuuaccaaa     1020

guccccgcag agacucugca cggcacagug accguagaag uucaauacgc ugguacggac     1080

gggccuugua agaucccagu gcagauggcc gucgauaugc agaccuuaac accggugggc     1140

cgccugauua cugcuaaucc cgucaucacc gagucuacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuau auagugauug gcguugggga caaaaagauc     1260

acucaccauu ggcaccgauc gggaucuacc auugguaaag ccuuugaagc aacagugaga     1320

ggcgccaaga ggauggcugu acugggggau accgcguggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8500
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 3 opt

<400>  8500
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgaugaa      540

aauagggcca aggucgaggu uacccccaac aguccucggg cugaagcaac gcugggcggg      600

uuuggcagcu ugggacucga cugcgagcca cgcacugguc uggauuucuc ugaccuguau      660

uaccucacca ugaauaacaa acauugguua gugcacaagg agugguuuca ugauauuccc      720

cugccuuggc acgccggggc ugacacagga acuccacacu ggaauaacaa agaagcccuu      780

guagaguuca aggacgcgca ugcaaagaga cagaccgugg ucguguuggg cucccaggaa      840

ggcgccguuc acacagcucu ggccggggca cuagaggcug aaauggaugg agccaaaggu      900

aggcucuuuu caggccaucu gaagugucgg cuuaaaaugg acaagcugcg cuugaaaggg      960

gugagcuaca gucucugcac cgcggccuuc acguuuacua agguccccgc agagacacug     1020

cacggaaccg ugacuguaga gguucaauau gcuggcacag augguccuug uaaagugcca     1080

gcccagaugg cugucgacau gcagaccuua acgccggugg ggcgacugau caccgccaau     1140

cccgucauua cagaaucuac ugagaacucc aagaugaugc ucgaacuuga uccuccauuc     1200

ggcgacagcu acaucgugau aggaguuggc gauaagaaaa uuacccacca uuggcacaga     1260

ucggggucua caaucggaaa ggcauuugag gccacuguga ggggugcuaa acggauggcg     1320

guacugggcg acaccgcaug ggauuucggg uccugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  8501
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 3 opt

<400>  8501
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgg cuaugaaacc      540

gaugagaaca gggccaaggu ggaagucacg cccaauaguc cucgggcuga ggcaacucug      600

ggaggguuug gcagcuuggg ucucgacugc gagccacgca ccgggcugga uuucucugac      660

cuguacuacc ucacaaugaa caauaaacau ugguuaguuc acaaggaaug guuucaugau      720

auuccccugc cuuggcacgc cggagcugac acuggcaccc cacacuggaa caauaaagag      780

gcccuugugg aguucaagga cgcgcaugca aagagacaga caguaguggu cuugggcucc      840

caggaagggg ccgugcacac cgcucuggcc ggagcacuag aagcugagau ggauggugcc      900

aaaggcaagc ucuuuucagg gcaucugaaa uguaggcuua agauggacaa acugcgguug      960

aagggaguua gcuauagucu cugcacggcg gccuucacuu uuacaaaagu gcccgcagag     1020

acccugcacg gcacugucac aguggaagua caauacgcug guaccgaugg gccuuguaag     1080

aucccaguuc agauggccgu ggacaugcag acguuaaccc cggucggccg ccugauuaca     1140

gcuaaccccg ugaucacuga gucuaccgaa aauuccaaga ugaugcucga gcuugauccu     1200

ccauucggag acagcuauau agucauuggc gugggggaua aaaagaucac acaccauugg     1260

caccgaucgg gaucuacuau ugguaaagcc uuugaagcaa ccguuagagg cgccaagagg     1320

auggcugugc ugggggacac agcgugggau uucggauccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8502
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 3 opt

<400>  8502
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu cccugagaug caucggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu ggucuuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau ugaguuaguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuaccugga caagcagagc gauacccagu auguuugcaa acgaacauug      360

guggaccgug gcugggguaa cgggugugga cucuuuggca agggcagucu ggugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uucaaccgga gaaucuugag      480

uacagaauca ugcugagcgu ccauggaucc cagcacucag guaugauagu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauca cgcccaacag uccucgggca      600

gaggccacuc uagggggauu uggcagccuc ggucuggauu gcgagccacg caccgggcug      660

gacuucucug aucucuauua cuuaacaaug aauaacaaac acuggcuggu ucauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcguuggu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

guccugggcu cccaggaagg ggcugugcau accgcccuag caggagcucu cgaagccgag      900

auggacggug cgaaaggcag gcugucaagc gggcaccuua agugucggcu gaaaauggau      960

aaguugcgcc ucaaaggagu uaguuacucu cugugcacgg ccgcauuuac uuucacaaag     1020

auccccgcug agaccuuaca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacgcugac cccggugggc     1140

cgacucauua cagcaaaucc cgucaucacu gaguccaccg aaaacagcaa gaugaugcuu     1200

gagcuggacc cuccauuugg agauucguac auagugauug gcgucgggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu auugguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu uuugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8503
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 3 opt

<400>  8503
gggagaaagc uuaccaugag caagaaagug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacag      180

gacaagccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

caaggagaag ccuacuuaga caaacagagc gauacccagu auguuugcaa gcgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca aaggcagucu cgugaccugc      420

gcaaaguucg ccuguuccaa gaaaaugacu gggaagucua uccagccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc caacacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaaa gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaagc acugguuagu ucauaaagaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaagg aggcgcuugu ggaguucaag gaugcacacg ccaaaagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaagggcag gcucucaagc gggcaccuga aaugucggcu uaagauggau      960

aaacugcgcu ugaagggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaaa     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaguaugc cgguaccgac     1080

gggccuugua agguaccagc ucaaauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga gaaaaagauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaaag ccuucgaagc uaccgugagg     1320

ggcgcgaagc ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8504
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 3 opt

<400>  8504
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu gguauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8505
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 3 opt

<400>  8505
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcaa gcucucaagc gggcaccuga aauguaggcu uaagauggau      960

aaacugcggu ugaagggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaaa     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua agguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgccugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga gaaaaagauu     1260

acacaucacu ggcaucgauc uggauccacu aucgguaaag ccuucgaagc uaccgugaga     1320

ggcgcgaaga ggauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8506
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 3 opt

<400>  8506
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauacgc agaccuuaac accggugggc     1140

cgacugauca cugcaaaucc cgucauaacc gaguccacag aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acucaucacu ggcauagauc uggauccacc aucgguaagg ccuucgaggc uacagugagg     1320

ggcgcgaaac ggauggcagu ucugggggac accgccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8507
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 3 opt

<400>  8507
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaaauu      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag guggaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ccauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ugaguucaag gaugcacacg ccaagagaca gacaguggua      840

guguugggcu cccaggaagg ggcuguccau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auccccgcug agacccugca cggcacuguu acaguggaag uccaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacguuaac cccgguuggc     1140

cgacugauaa cagcaaaucc cgugauuacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgucauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8508
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 3 opt

<400>  8508
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgcggc guauggcagu ucugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8509
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 3 opt

<400>  8509
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacaugguuu cggauucacg cuguccaacu      300

cagggagagg cuuacuuaga caagcagagc gauacccagu augugugcaa acgaacacug      360

gucgaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gccaaauucg cauguuccaa gaagaugacu gggaaaucua uccaaccgga aaaucuggag      480

uacagaauaa ugcuuagcgu gcauggaucc cagcacucag guaugaucgu caacgauaca      540

ggccaugaaa ccgacgagaa uagggccaag guugaaauua cgcccaacag uccucgggcu      600

gaggcaacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu gcauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcug acacuggcac cccacauugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcacg caaagagaca gacagugguc      840

guguugggcu cccaggaagg ggccguucau accgcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg cggccuuuac uuucacaaag     1020

auucccgcag agacccugca cggcacuguc acaguggaag uacaauaugc ugguaccgac     1080

gggccuugua aaguuccagc ccagauggcu guggauaugc agacguuaac cccggucggc     1140

cgacugauca cagccaaucc cgugauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg cauucgaggc caccguuagg     1320

ggcgcuaaac ggauggcggu gcugggggac acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8510
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 3 opt

<400>  8510
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccauug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgugauug gcgucgggga aaagaaaauc     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg ccuucgaggc uacugugagg     1320

ggcgcgaaac ggauggcagu ucugggggac accgccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8511
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 3 opt

<400>  8511
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcauugggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8512
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 3 opt

<400>  8512
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cuggcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu ggucuuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccca cuguggauau cgaguuaguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccuacu      300

cagggagaag ccuaccugga caagcagagc gauacccagu auguuugcaa acgaacauug      360

guggaccgug gcugggguaa cgggugugga cucuuuggca agggcagucu ggugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccaga gaaucuugag      480

uacagaauaa ugcugagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgccgaacag uccccgggca      600

gaggccacuc uagggggauu uggcagccuc ggucuggauu gcgagccucg caccgggcug      660

gacuucucug aucucuauua cuuaacaaug aauaacaaac acuggcuggu ucauaaggaa      720

ugguuucacg acaucccacu ucccuggcac gcuggagccg acacuggcac cccucauugg      780

aauaacaaag aggcguuggu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

guccugggcu cccaggaagg ggcugugcau accgcccuag caggagcucu cgaagccgag      900

auggacggug cgaaaggcag gcugucaagc gggcaccuua agugucggcu gaaaauggau      960

aaguugcgcc ucaaaggagu uaguuacucu cugugcacgg ccgcauuuac uuucacaaag     1020

auuccagcug agaccuuaca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggcccugua aaguaccugc ucagauggcc guugauaugc agacgcugac cccagugggc     1140

cgacucauca cagcaaaucc ggucauaacu gaguccaccg aaaacagcaa gaugaugcuu     1200

gagcuggauc ccccuuuugg agacucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu uuugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8513
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 3 opt

<400>  8513
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggca cccaggaagg ggcugugcau acggcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucuccuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uagcuacagu cucugcacug ccgcauuuac auucaccaag     1020

auucccgcug agacucugca cggcacagug accgucgaag ugcaauaugc cgguacggac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agaccuuaac accggugggc     1140

cgacugauca cugcaaaucc cgucauaacc gagucuacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauagcuac auugugaucg gcgucgggga aaagaaaauu     1260

acucaucacu ggcauagauc gggaucuacc aucgguaagg ccuucgaggc uacagugagg     1320

ggcgcgaaac ggauggcagu ucugggggac accgccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8514
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 3 opt

<400>  8514
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguauuagc ucagauggcc guugauaugc agacgcugac cccagugggc     1140

cgacucauca cagcaaaucc ggucauaacu gaguccaccg aaaacagcaa gaugaugcuu     1200

gagcuggauc ccccuuuugg agacucguac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu uuugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8515
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 3 opt

<400>  8515
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaagcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaggccgag      900

auggauggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggac      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug aaacccugca cggcacugug acagucgagg ugcaauaugc cgguaccgau     1080

gggccuugua aaguaccagc ucagauggcc guugacaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaauccaccg agaacagcaa gaugaugcuc     1200

gaacuugauc cuccauuugg agacucguac auugugaucg gcgucgggga gaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggau acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8516
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 3 opt

<400>  8516
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccgcuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacugggcug      660

gacuucucug aucuguauua ccucaccaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcgg acacaggcac uccacauugg      780

aauaacaaag aggcacuugu ggaguucaag gaugcccacg cuaagagaca gaccguagug      840

gucuugggcu cccaggaagg ggccgugcau acagcacugg cuggagcccu agaagcggag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcaccg cagcuuuuac guucacuaag     1020

auucccgccg agacacugca cggcaccgug acugucgaag ugcaauaugc ugguacagac     1080

gggccuugua aaguaccagc ccagauggca guugauaugc agaccuuaac gccggugggc     1140

cgacugauca ccgccaaucc cgucauaaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg cuuucgaggc gacugugagg     1320

ggcgcaaaac ggauggccgu ucugggggac accgcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8517
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 3 opt

<400>  8517
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc aguacgaccg ugagcaauau ggccgaagua      240

cgguccuacu gcuaugaggc gacaauuucu gacaucgcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacagcugua      840

guguugggcu cccaggaagg ggccguccau accgcacugg cuggagcccu agaagcggag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg cagcuuuuac uuucacaaag     1020

auccccgccg agacccugca cggcacuguu acaguggaag uccaauaugc ugguaccgac     1080

gggccuugua aagugccagc ccagauggca guagauaugc agacguuaac cccgguuggc     1140

cgacugauaa cagccaaucc cgugauuacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgucauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg cuuucgaggc gaccgucagg     1320

ggcgcaaaac ggauggccgu gcugggggac acagcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8518
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 3 opt

<400>  8518
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucuccaag     1020

auucccgcug agacacugca cggcaccgug acugaagagg uccaauaugc cgguacagac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agaccuuaac gccgguuggc     1140

cgacugauca ccgcaaaucc cgugauaaca gaaagcacug agaacucgaa gaugaugcuc     1200

gaacuugacc cuccauuugg agauucuuac auugucaucg gcguggggga gaagaaaauu     1260

acccaucacu ggcauagauc cggaucaaca aucgguaagg ccuucgaggc uacugucagg     1320

ggcgcgaaac ggauggcagu gcugggggac accgccuggg auuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8519
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 3 opt

<400>  8519
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaau uacaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacgcugac cccgguuggc     1140

cgacucauca cagcaaaucc cgugauaacu gaguccaccg aaaacagcaa gaugaugcuu     1200

gagcuggacc cuccauuugg agauucguac auugucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu guugggggac acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8520
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 3 opt

<400>  8520
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcauc ucuaacaggg acuucguuga ggggauguca      120

ggagguaccu ggguggaugu agugcuggaa cacggcgggu gugucacagu gauggcacaa      180

gacaaaccua cuguugauau ugaguuggug acgaccacag ucaguaauau ggccgaagug      240

cggagcuacu gcuaugaggc guccaucucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uacaaccgga gaaucuggag      480

uacagaauca ugcuuagcgu ccauggaucc cagcacucag guaugauugu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag guggaaauca cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcauuuug ggucucgauu gcgagccacg caccgggcug      660

gacuucagcg aucuguauua ccucacaaug aauaacaaac acugguuagu ccauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ugaguucaag gaugcacacg ccaagagaca gacaguggua      840

guguugggcu cucaggaagg ggcuguccau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucuccuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gagcuacagu cucugcacgg ccgcauuuac uuucacaaag     1020

auacccgcug agacccugca cggcacuguu acaguggaag uccaauaugc cgguaccgac     1080

gggccuugua aagugccagc ucagauggcc guagauaugc agacguuaac cccgguuggc     1140

cgacugauua cagcaaaucc cgugaucacu gagucuaccg aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauagcuac auugucaucg gcguggggga aaagaaaauc     1260

acacaucacu ggcauagauc gggaucuacu auugguaagg ccuucgaggc uaccgucagg     1320

ggcgcgaaac ggauggcagu gcugggggac acagccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8521
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 3 opt

<400>  8521
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug uuaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gaguuacucu cucugcacgg cggccuuuac uuucacaaag     1020

auucccgcag agacccugca cggcacuguc acaguggaag uacaauaugc ugguaccgac     1080

gggccuugua aaguuccagc ccagauggcu guggauaugc agacguuaac cccggucggc     1140

cgacugauca cagccaaucc cgugauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagauc uggauccacu aucgguaagg cauucgaggc caccguuagg     1320

ggcgcuaaac ggauggcggu gcugggggac acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8522
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 3 opt

<400>  8522
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cggugcugac     1080

gggccuugua aaguaccagc ccagauggca guugauaugc agaccuuaac gccggugggc     1140

cgacugauca ccgccaaucc cgucauaaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg cuuucgaggc gacugugagg     1320

ggcgcaaaac ggauggccgu ucugggggac accgcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8523
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 3 opt

<400>  8523
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac acuggagcug acaccggcac accacauugg      780

aauaacaaag aggcccuugu ggaguucaag gaugcgcacg caaagagaca gaccguagug      840

gucuugggcu cccaggaagg ggccgugcau acggcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacug cggccuuuac auucaccaag     1020

auucccgcag agacucugca cggcacagug accgucgaag ugcaauaugc ugguacggac     1080

gggccuugua aaguaccagc ccagauggcu guugauaugc agaccuuaac accggugggc     1140

cgacugauca cugccaaucc cgucauaacc gagggaacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg cgauagcuac auugugaucg gggucggaga aaagaaaauu     1260

acucaucacu ggcauagauc ggguucuacc aucggcaagg cauucgaggc cacagugagg     1320

ggagcuaaac ggauggcggu ucugggggac accgcauggg auuuuggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8524
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 3 opt

<400>  8524
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagc aauggcccaa      180

gacaaaccua cuguugauau cgaguuggug acgaccacag ucaguaauau ggcggaagug      240

cggagcuacu gcuaugaggc uuccauuucu gacauggccu cggauucacg cuguccaacu      300

cagggagaag cauacuuaga caagcagagc gauacccagu auguaugcaa acgaacacug      360

guugaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gccaaauucg cuuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugcuggu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcaaag guggaaauca cgcccaacag uccucgggcc      600

gaggcuacuc ucgggggauu uggcagcuug ggucuggauu gcgagccacg caccgggcug      660

gacuucucug aucucuauua cuuaacaaug aauaacaaac acuggcuggc ccauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gcgggagcag ccacuggcac cccacauugg      780

aauaacaaag aggcuuuggu cgaguucaag gacgcccacg caaagagaca gacaguugug      840

guacugggcu cccaggaagg ggcugugcau accgcccuag cgggagcccu cgaagcagag      900

auggauggug cuaaaggcag gcugucaagc gggcaccuua agugucggcu gaaaauggac      960

aaguugcgcc ucaaaggagu caguuacucu cugugcacgg ccgcuuuuac uuucacaaag     1020

auccccgccg agaccgugga uggcacuguu acaguggaag gucaauaugg gggcaccgac     1080

ggaccuugua aagucccagc acagauggcc guggauaugc agacguuaac cccgguaggc     1140

cgacugauua cagcuaaucc cguuaucacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg ggauucguac auagugauug gagucgguga aaagaaaauc     1260

acacaccauu ggcacagauc uggcuccacu auugggaagg cguucgaggc aaccgugagg     1320

ggagccaaac ggauggcugu ccugggcgac acagccuggg auuuugguuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8525
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 3 opt

<400>  8525
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccug ccguggauau cgaguugguc acuacgaccg ugaguaauau ggcggaagua      240

cggagcuacu gcuaugaggc uuccauuucu gacauggccu cggauucacg cuguccaaca      300

cagggagaag cauacuuaga caagcagagc gauacucagu auguuugcaa acgaacccug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgucacaugc      420

gccaaauucg cuuguuccaa gaagaugacc gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu gcauggaucc cagcacucag guaugaucgu gaacgauacu      540

ggccaugaaa cagacgagaa uagggcaaag gucgaaauua cccccaacag uccucgggcc      600

gaggcuacgc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacugggcug      660

gacuucucug aucuguauua ccucaccaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcgg acacaggcac uccacauugg      780

aauaacaaag aggcacuugu ggaguucaag gaugcccacg cuaagagaca gaccguagug      840

gucuugggcu cccaggaagg ggccgugcau acagcacugg cuggagcccu agaagcggag      900

auggacggug ccaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcaccg cagcuuuuac guucacuaag     1020

auucccgccg agacacugca cggcaccgug acugucgaag ugcaauaugc ugguacagac     1080

gggccuugua aaguaccagc ccagauggca guugauaugc agaccuuaac gccggugggc     1140

cgacugauca ccgccaaucc cgucauaaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg cuuucgaggc gacugugagg     1320

ggcgcaaaac ggauggccgu ucugggggac accgcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8526
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 3 opt

<400>  8526
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu aacaauaggg acuucgugga ggggaugucu      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugucaaacau ggccgaagua      240

cggaguuacu gcuaugaggc gagcauuucc gacauggcuu cugauucgcg cuguccaacu      300

cagggagaag ccuacuuaga caagcaguca gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa ugggugugga cuauuuggca agggcagccu cgugaccugc      420

gcaaaauucg ccuguaguaa gaagaugacu gggaaaucca uccaaccgga gaaccuggag      480

uacagaauaa ugcuuucugu ccauggaagc cagcacuccg guaugaucgu gaaugauaca      540

ggccaugaaa ccgacgagaa cagggcuaag gucgaaauua cgcccaauuc accucgggca      600

gaggccacuc ugggaggguu uggcaguuug ggucucgauu gcgagccacg caccgggcug      660

gacuucagcg aucuguauua ccucacaaug aacaauaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aacaauaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cucaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucuccuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uagcuacagu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaccc cgucauaacu gagucuaccg aaaauuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauagcuac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc gggaucuacu aucgguaagg ccuucgaggc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggac acagccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8527
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 3 opt

<400>  8527
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcgccg cagcuuuuac guucacuaag     1020

auucccgccg agacacugca cggcaccgug acugucgaag ugcaauaugc ugguacagac     1080

gggccuugua aaguaccagc ccagauggca guugauaugc agaccuuaac gccggugggc     1140

cgacugauca ccgccaaucc cgucauaaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg cuuucgaggc gacugugagg     1320

ggcgcaaaac ggauggccgu ucugggggac accgcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8528
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 3 opt

<400>  8528
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauauu      540

ggccaugaaa cagacgagaa uagggcuaag gucgaaauca cccccaacag uccucgggca      600

gaggccacgc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacugggcug      660

gacuucucug aucuguauua ccucaccaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gcuggagccg acacaggcac uccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gaccguagug      840

gucuugggcu cccaggaagg ggcugugcau acagcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcaccg ccgcauuuac guucacuaag     1020

auccccgcug agacacugca cggcaccgug acugucgaag ugcaauaugc cgguacagac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agaccuuaac gccggugggc     1140

cgacugauaa ccgcaaaucc cgucauuaca gaguccacug aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac aucgugauug gcgucgggga caagaaaauc     1260

acccaucacu ggcauagauc uggauccaca aucgguaagg ccuucgaagc uacugugagg     1320

ggcgcgaaac ggauggcagu ucugggggau accgccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8529
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 3 opt

<400>  8529
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauucg ccuguuccaa gaagaugacu gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa uagggcuaag gucgaaauua cgcccaacag uccucgggca      600

gaggccacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagagaca gacaguagug      840

gucuugggcu cccaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggcag gcucucaagc gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu uaguuacucu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

cgacugauca cagcaaaucc cgucauaacu gaguccaccg aaaacagcaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauucguac auugugaucg gcgucgggga caagaaaauu     1260

acacaucacu gguauagauc uggauccacu aucgguaagg ccuucgaagc uaccgugagg     1320

ggcgcgaaac ggauggcagu ucugggggau acagccuggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8530
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 3 opt

<400>  8530
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauacc      540

ggccaugaaa cggacgagaa uagggccaag gucgaaguua cucccaacag uccucgggcu      600

gaggcaaccc ugggaggguu uggcagcuug ggucucgauu gcgagccacg cacagggcug      660

gacuucucug aucuguauua ccucacuaug aauaacaaac acugguuagu gcauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gccggagcug acaccggcac accacauugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcacg caaagagaca gaccgugguc      840

guguugggcu cccaggaagg ggccguucau acggcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucuuuuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gagcuacagu cucugcacug cggccuucac auuuaccaag     1020

guccccgcag agacucugca cggcacagug accguagaag uucaauaugc ugguacggac     1080

gggccuugua aagugccagc ccagauggcu gucgauaugc agaccuuaac accggugggc     1140

cgacugauca cugccaaucc cgucauuacc gagucuacag aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuac aucgugauag gcguugggga caagaaaauu     1260

acucaucacu ggcauagauc gggaucuacc aucgguaagg cauuugaagc cacagugagg     1320

ggcgcuaaac ggauggcggu acugggggau accgcauggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8531
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 3 opt

<400>  8531
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauauu      540

ggccaugaaa ccgacgagaa uagggccaag gucgaaguua cgcccaacag uccucgggcu      600

gaggcaacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu gcauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcug acacuggcac cccacauugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcacg caaagagaca gacagugguc      840

guguugggcu cccaggaagg ggccguucau accgcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucuuuuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gagcuacagu cucugcacgg cggccuucac uuuuacaaag     1020

guccccgcag agacccugca cggcacugug acaguagaag uucaauaugc ugguaccgac     1080

gggccuugua aagugccagc ccagauggcu gucgauaugc agacguuaac cccggugggc     1140

cgacugauua cagccaaucc cgucaucacu gagucuaccg aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuac auagugauug gcguugggga caagaaaauc     1260

acacaucacu ggcauagauc gggaucuacu auugguaagg cauuugaagc caccgugagg     1320

ggcgcuaaac ggauggcggu acugggggau acagcauggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8532
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 3 opt

<400>  8532
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauauu      540

ggccaugaaa ccgacgagaa uagggccaag gucgaaguua cgcccaacag uccucgggcu      600

gaggcaacuc ugggaggguu uggcagcuug ggucucgauu gcgagccacg caccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaaac acugguuagu gcauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcug acacuggcac cccacauugg      780

aauaacaaag aggcccuugu agaguucaag gaugcgcacg caaagagaca gacagugguc      840

guguugggcu cccaggaagg ggccguucau accgcucugg ccggagcacu agaagcugag      900

auggacggug ccaaaggcag gcucuuuuca gggcaccuga agugucggcu uaaaauggau      960

aagcugcgcu ugaaaggagu gagcuacagu cucugcacgg cggucuguac ugccgcaaag     1020

gugcccgcug agacacugca cggcaccgua acuguugaag ugcaauaugc cgguacagac     1080

gggccuugca aagucccagc ucagauggcc guggauaugc agaccuuaac gccggucggc     1140

cgacugauua ccgcaaaucc cgugaucaca gagucuacug aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauucgg agauagcuac auaguuauug gcguggggga caagaaaauc     1260

acccaucacu ggcauagauc gggaucuaca auugguaagg ccuuugaagc uacuguaagg     1320

ggcgcgaaac ggauggcagu ccugggggau accgccuggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8533
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 3 opt

<400>  8533
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucagaug cauuggcguu ucuaacaggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cggagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg cuguccaacu      300

cagggagaag ccuacuuaga caagcagagc gauacccagu auguuugcaa acgaacacug      360

guggaccgug gcugggguaa cgggugugga cuauuuggca agggcagucu cgugaccugc      420

gcaaaauuca cuuguuccaa gaagaugaca gggaaaucua uccaaccgga gaaucuggag      480

uacagaauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgaugaa      540

aauagggcca aggucgaggu uacccccaac aguccucggg cugaagcaac gcugggcggg      600

uuuggcagcu ugggacucga cugcgagcca cgcacugguc uggauuucuc ugaccuguau      660

uaccucacca ugaauaacaa acauugguua gugcacaagg agugguuuca ugauauuccc      720

cugccuuggc acuccggggc cgacacagaa acuccacacu ggaauaacaa agaggcucuu      780

guagaguuca aggacgccca ugcgaagaga cagaccgugg ucguguuggg aucacaggaa      840

ggcgcaguuc acacagcccu ggcuggcgcc cuagaagcag agauggaugg ggcuaaagga      900

aggcucagca guggucaucu gaagugucgg cuuaaaaugg acaagcugcg cuugaaaggc      960

gugucuuacu cccucugcac cgccgcguuu acguucacua agguccccgc cgagacacug     1020

cacgggaccg ugacuguaga aguucaauau gcaggacgag auggcccuug uaaagugcca     1080

gcucagaugg ccgucgacau gcagacauua accccggugg guagacugau cacggcuaau     1140

cccgucauua ccgagagcac agaaaacucg aagaugaugc ucgagcuuga uccuccauuu     1200

ggggacucuu acaucgugau aggcguugga gauaagaaaa uuacucacca uuggcacagg     1260

uccggcucaa ucauugggaa ggccuucgaa gcaaccgugc ggggagccaa acguauggcu     1320

guacugggug acacagcgug ggauuuuggc aguugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  8534
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 3 opt

<400>  8534
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauugu ucucauagug       60

cccgccuauu ccaucucuug cauuggcgua ucaaacagag accuugugga ggggaugagu      120

ggagguaccu gggucgaugu gguucuggaa cacggcgggu gugugacaga gauggcucaa      180

gacaaaccua cugucgauau cgaauuggug acgaugaccg uaagcaauau ggcagagguu      240

agguccuacu gcuaugaagc cucuuuaucg gacauggcgu cagcuagccg guguccaaca      300

cagggagagc cgagucugga uaagcagucc gacacucagu cugugugcaa acgcacccua      360

ggcgaucgag guugggggaa cggauguggc auuuucggca aggggagccu cgucacaugc      420

uccaaauuua ccuguugcaa gaagaugccc ggaaaaucaa uccaaccuga gaaucuggaa      480

uaccguauaa ugcuuccagu gcaugguagu cagcacagcg gcaugaucgu gaacgacauu      540

gggcaugaga cugaugaaaa uagagccaag gucgagguua cacccaacuc uccuagggca      600

gaggccaccc ugggaggcuu cgguuccuug gggcucgacu gugaaccacg gacgggacug      660

gauuuuucag accuguauua ccucacuaug aauaacaaac acugguuagu gcauaaggag      720

ugguuccacg auaucccccu gccuuggcac gcuggcgcag acaccggcac accacauugg      780

aauaacaaag aagcccuugu agaguuuaag gacgcucacg ccaagcgcca gacugugguc      840

guguugggga gccaggaagg agcgguucau accgcacugg ccggugcucu agaggccgag      900

auggauggcg caaaagggag acucuucagu ggacaccuga agugcaggcu uaaaauggac      960

aagcugcggu ugaaaggcgu gucuuauucc cucuguacag cugccuuuac cuucacgaag     1020

gucccggcgg aaacucugca cgguacagug accguagagg uucaaagcgc cgggacugau     1080

ggccccugca aagugccugc acagauggcu gucgacaugc agacauuaac cccaguggga     1140

cgccugauua cggccaaucc cgucaucacc gaaucgacag agaacucuaa gaugaugcuc     1200

gaacuugauc cuccauuugg cgacuccuac auagugauug ggguuggaga uaagaaaauc     1260

acucaucacu ggcaucgauc agguaguacc auuggcaagg cuuucgaggc cacagugaga     1320

ggggcaaaaa ggauggccgu acugggagac acugcuuggg auuuuggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8535
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 3 opt

<400>  8535
gggagaaagc uuaccaugag acagaaggug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuaua gcaucaggug cauuggcguu uccaaccggg acuucgugga ggggaugucu      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaaaccua cuguggauau cgaguugguc acgaccacag ugucaaauau ggccgaagua      240

cgcaguuacu gcuaugaggc gagcauuucc gacauggcuu cugauucgcg auguccaacu      300

cagggagaag ccuacuuaga caagcaguca gauacccagu auguuugcaa acguacacug      360

guggacagag gcugggguaa cgggugugga cuauuuggca agggcagccu cgugaccugc      420

gcaaaauucg ccuguaguaa gaagaugacu gggaaaucca uccaaccgga gaaucuggag      480

uacaggauaa ugcuuucugu ccauggaagc cagcacuccg guaugaucgu gaacgauaca      540

ggccaugaaa ccgacgagaa ucgggcuaag gucgaaauua cgcccaacuc accucgcgca      600

gaggccacuc ugggaggguu uggcaguuug ggucucgauu gcgagccaag aaccgggcug      660

gacuucagcg aucuguauua ccucacaaug aauaacaaac acugguuagu ucauaaggaa      720

ugguuucacg acaucccccu gccuuggcac gcuggagccg acacuggcac cccacauugg      780

aauaacaaag aggcgcuugu ggaguucaag gaugcacacg ccaagaggca gacaguagug      840

gucuugggcu cucaggaagg ggcugugcau accgcccugg caggagcucu agaagccgag      900

auggacggug cgaaaggccg gcucuccuca gggcaccuga agugucgccu uaaaauggau      960

aagcugcgau ugaaaggagu uagcuacagu cucugcacgg ccgcauuuac uuucacaaag     1020

auucccgcug agacccugca cggcacugug acagucgaag ugcaauaugc cgguaccgac     1080

gggccuugua aaguaccagc ucagauggcc guugauaugc agacguuaac cccggugggc     1140

agacugauca cagcaaaucc cgucauaacu gagucuaccg aaaacuccaa gaugaugcuc     1200

gagcuugacc cuccauuugg agauagcuac auugugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagguc gggaucuacu aucgguaagg ccuucgaggc uaccgugcgg     1320

ggcgcgaaac guauggcagu ucugggggac acagccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8536
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 3 opt

<400>  8536
gggagaaagc uuaccaugag ccagagagug aucuaccugg ucaugauucu ccuuauagcc       60

cccgcuuauu ccaucaggug cauuggcguu ucuaaccggg acuucgugga ggggauguca      120

ggagguaccu ggguagaugu gguccuggaa cacggcgggu gugugacagu uauggcacaa      180

gacaagccua cuguggauau cgaguugguc acgaccacag ugaguaauau ggccgaagua      240

cgcagcuacu gcuaugaggc guccauuucu gacauggcuu cggauucacg auguccaacu      300

cagggagaag ccuacuuaga caaacagagc gauacccagu auguuugcaa gcguacacug      360

guggacagag gcugggguaa cgggugugga cuauuuggca aaggcagucu cgugaccugc      420

gcaaaguuca cuuguuccaa aaagaugaca gggaagucua uccaaccgga gaaucuggag      480

uacaggauaa ugcuuagcgu ccauggaucc cagcacucag guaugaucgu gaacgauauu      540

ggccaugaaa ccgacgagaa ucgggccaaa gucgaaguua cgcccaacag uccucgcgcu      600

gaggcaacuc ugggaggguu uggcagcuug ggucucgauu gcgagccaag aaccgggcug      660

gacuucucug aucuguauua ccucacaaug aauaacaagc acugguuagu gcauaaagaa      720

ugguuucacg acaucccccu gccuuggcac gccggagcug acacuggcac cccacauugg      780

aauaacaagg aggcccuugu agaguucaaa gaugcgcacg caaagaggca gacagugguc      840

guguugggcu cccaggaagg ggccguucau accgcucugg ccggagcacu agaagcugag      900

auggacggug ccaagggccg gcucuuuuca gggcaccuga aaugucgcau uaagauggau      960

aaacuucgac ugaagggagu gagcuacagu uugugcacgg cggccuucac uuuuucuaaa     1020

guccccgcag agacacucca cggcaccgug acuguagaag uucaauaugc ugguagagac     1080

gggccuugua aggugccagc ccagauggcu gucgauaugc agacacugac cccggugggc     1140

agguuaauca cggccaaucc cauaauuacc gaguccacag aaaacagccg gaugaugcug     1200

gagcucgacc cuccauucgg agauucguac aucgucauug gcguggggga caaaaagauc     1260

acucaucacu ggcaucguuc uggauccacc aucgguaagg cauuugaagc cacaguucgc     1320

ggcgcuaaaa gaauggcggu gcuuggggau acugcauggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8537
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  8537
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8538
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 3 opt

<400>  8538
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8539
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 3 opt

<400>  8539
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1080

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8540
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 3 opt

<400>  8540
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8541
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  8541
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8542
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 3 opt

<400>  8542
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8543
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 3 opt

<400>  8543
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8544
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 3 opt

<400>  8544
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8545
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 3 opt

<400>  8545
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8546
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 3 opt

<400>  8546
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8547
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 3 opt

<400>  8547
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      540

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug      600

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc      840

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      900

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug      960

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1140

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1320

auggccgugc ugggcgacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8548
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 3 opt

<400>  8548
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8549
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       3 opt

<400>  8549
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccugag cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8550
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 3
       opt

<400>  8550
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccugag cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8551
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 3 
       opt

<400>  8551
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccugag cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8552
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F399S) mRNA Design 3 
       opt

<400>  8552
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccugag cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8553
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       3 opt

<400>  8553
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugcagg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8554
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 3
       opt

<400>  8554
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugcagg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8555
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 3 
       opt

<400>  8555
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugcagg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8556
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8556
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8557
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8557
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8558
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 3 opt

<400>  8558
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc acccugggca aggccuucag caccacccug     1800

aagggcgccc agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc     1860

ggcguguuca acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc     1920

cuguucggcg gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug     1980

ggcgugaacg ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug     2040

guguuccugg ccaccaacgu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8559
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8559
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8560
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  8560
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8561
<211>  2362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8561
gggagaaagc uuaccaugau caucaacgcc agaaaggaga agaagagaag aggcgccgac       60

accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga      120

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      180

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      360

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      420

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      480

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      540

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      600

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      660

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      720

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      780

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      840

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      900

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac     1020

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1080

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1140

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1200

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1380

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1440

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1500

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1620

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1680

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1740

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1800

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1860

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1920

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     2040

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2100

cugagcaccg ccgugagcgc cgacgugggc ugcagcgugg acuucagcaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2340

ucuuuucaga gccaccagaa uu                                              2362


<210>  8562
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8562
gggagaaagc uuaccaugau caacgccaga aaggagaaga agagaagagg cgccgacacc       60

agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga      120

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      180

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      240

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      360

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      420

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      480

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      540

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      600

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      660

accugggugg acaucgugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      720

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      780

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      840

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      900

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      960

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga     1020

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1080

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1140

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1200

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1380

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1440

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1500

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1620

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1680

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1740

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1800

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1860

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1920

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1980

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     2040

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2100

agcaccgccg ugagcgccga cgugggcugc agcguggacu ucagcaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  8563
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8563
gggagaaagc uuaccaugaa cgccagaaag gagaagaaga gaagaggcgc cgacaccagc       60

gugggcaucg ugggccugcu gcugaccacc gccauggccg ccgaggugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcagc guggacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8564
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8564
gggagaaagc uuaccauggc cagaaaggag aagaagagaa gaggcgccga caccagcgug       60

ggcaucgugg gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc      120

gccuacuaca uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc      180

cugggcauga acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc      240

augagcuacg agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc      360

agaagaagca gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      420

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      480

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      540

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      600

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      660

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      720

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      780

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      840

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      900

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug     1020

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc     1080

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8565
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8565
gggagaaagc uuaccaugag aaaggagaag aagagaagag gcgccgacac cagcgugggc       60

aucgugggcc ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc      120

uacuacaugu accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug      180

ggcaugaaca agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug      240

agcuacgagu gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga      360

agaagcagaa gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      420

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      480

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      540

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      600

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      660

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      720

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      780

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      840

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      900

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      960

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug     1020

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac     1080

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc     1140

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1200

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1380

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1440

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1500

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1620

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1680

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1740

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1800

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1860

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1980

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     2040

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     2100

gugagcgccg acgugggcug cagcguggac uucagcaagu gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2220

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  8566
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8566
gggagaaagc uuaccaugaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8567
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8567
gggagaaagc uuaccaugga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgacgugg gcugcagcgu ggacuucagc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  8568
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8568
gggagaaagc uuaccaugaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gacgugggcu gcagcgugga cuucagcaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  8569
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8569
gggagaaagc uuaccaugaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugca gcguggacuu cagcaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8570
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8570
gggagaaagc uuaccaugag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcagcg uggacuucag caagugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8571
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8571
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcagcgugg acuucagcaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8572
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8572
gggagaaagc uuaccauggg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

agcguggacu ucagcaagug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8573
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8573
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcagc     2100

guggacuuca gcaagugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8574
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8574
gggagaaagc uuaccaugga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcagcgug     2100

gacuucagca agugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8575
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8575
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cagcguggac     2100

uucagcaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8576
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8576
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgccg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cagcguggac     2100

uucagcaagu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8577
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8577
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccgc cgugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcagcgugg acuucagcaa gugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8578
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8578
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcggcgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcagc guggacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8579
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8579
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcgccgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcagc guggacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8580
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8580
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8581
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8581
gggagaaagc uuaccaugau caucaacgcc agaaaggaga agaagagaag aggcgccgac       60

accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga      120

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      180

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      360

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      420

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      480

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      540

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      600

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      660

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      720

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      780

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      840

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      900

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac     1020

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1080

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1140

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1200

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1380

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1440

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1500

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1620

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1680

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1740

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1800

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1860

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1920

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     2040

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2100

cugagcaccg ccgugagcgc cgacgugggc ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  8582
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8582
gggagaaagc uuaccaugau caacgccaga aaggagaaga agagaagagg cgccgacacc       60

agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga      120

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      180

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      240

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      360

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      420

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      480

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      540

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      600

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      660

accugggugg acaucgugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      720

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      780

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      840

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      900

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      960

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga     1020

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1080

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1140

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1200

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1380

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1440

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1500

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1620

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1680

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1740

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1800

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1860

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1920

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1980

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     2040

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2100

agcaccgccg ugagcgccga cgugggcugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  8583
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8583
gggagaaagc uuaccaugaa cgccagaaag gagaagaaga gaagaggcgc cgacaccagc       60

gugggcaucg ugggccugcu gcugaccacc gccauggccg ccgaggugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8584
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8584
gggagaaagc uuaccauggc cagaaaggag aagaagagaa gaggcgccga caccagcgug       60

ggcaucgugg gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc      120

gccuacuaca uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc      180

cugggcauga acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc      240

augagcuacg agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc      360

agaagaagca gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      420

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      480

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      540

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      600

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      660

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      720

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      780

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      840

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      900

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug     1020

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc     1080

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8585
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8585
gggagaaagc uuaccaugag aaaggagaag aagagaagag gcgccgacac cagcgugggc       60

aucgugggcc ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc      120

uacuacaugu accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug      180

ggcaugaaca agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug      240

agcuacgagu gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga      360

agaagcagaa gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      420

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      480

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      540

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      600

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      660

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      720

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      780

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      840

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      900

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      960

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug     1020

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac     1080

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc     1140

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1200

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1380

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1440

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1500

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1620

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1680

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1740

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1800

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1860

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1980

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     2040

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     2100

gugagcgccg acgugggcug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8586
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8586
gggagaaagc uuaccaugaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8587
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8587
gggagaaagc uuaccaugga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgacgugg gcugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8588
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8588
gggagaaagc uuaccaugaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gacgugggcu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8589
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8589
gggagaaagc uuaccaugaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

gugggcugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8590
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8590
gggagaaagc uuaccaugag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacgug     2100

ggcugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8591
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8591
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8592
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8592
gggagaaagc uuaccauggg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cgugggcugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8593
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8593
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgacgu gggcugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8594
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8594
gggagaaagc uuaccaugga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacguggg cugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8595
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8595
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8596
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8596
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgccg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acgugggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8597
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8597
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccgc cgugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacgugggc     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8598
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8598
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcggcgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8599
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8599
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcgccgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacgu gggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8600
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8600
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8601
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8601
gggagaaagc uuaccaugau caucaacgcc agaaaggaga agaagagaag aggcgccgac       60

accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga      120

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      180

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      360

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      420

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      480

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      540

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      600

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      660

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      720

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      780

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      840

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      900

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac     1020

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1080

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1140

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1200

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1380

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1440

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1500

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1620

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1680

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1740

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1800

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1860

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1920

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     2040

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2100

cugagcaccg ccgugagcgc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8602
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8602
gggagaaagc uuaccaugau caacgccaga aaggagaaga agagaagagg cgccgacacc       60

agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga      120

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      180

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      240

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      360

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      420

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      480

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      540

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      600

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      660

accugggugg acaucgugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      720

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      780

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      840

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      900

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      960

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga     1020

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1080

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1140

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1200

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1380

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1440

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1500

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1620

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1680

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1740

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1800

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1860

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1920

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1980

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     2040

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2100

agcaccgccg ugagcgccga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8603
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8603
gggagaaagc uuaccaugaa cgccagaaag gagaagaaga gaagaggcgc cgacaccagc       60

gugggcaucg ugggccugcu gcugaccacc gccauggccg ccgaggugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8604
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8604
gggagaaagc uuaccauggc cagaaaggag aagaagagaa gaggcgccga caccagcgug       60

ggcaucgugg gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc      120

gccuacuaca uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc      180

cugggcauga acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc      240

augagcuacg agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc      360

agaagaagca gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      420

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      480

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      540

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      600

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      660

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      720

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      780

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      840

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      900

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug     1020

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc     1080

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     2100

gccgugagcg ccgacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8605
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8605
gggagaaagc uuaccaugag aaaggagaag aagagaagag gcgccgacac cagcgugggc       60

aucgugggcc ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc      120

uacuacaugu accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug      180

ggcaugaaca agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug      240

agcuacgagu gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga      360

agaagcagaa gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      420

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      480

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      540

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      600

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      660

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      720

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      780

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      840

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      900

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      960

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug     1020

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac     1080

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc     1140

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1200

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1380

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1440

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1500

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1620

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1680

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1740

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1800

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1860

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1980

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     2040

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     2100

gugagcgccg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8606
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8606
gggagaaagc uuaccaugaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccgacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8607
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8607
gggagaaagc uuaccaugga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8608
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8608
gggagaaagc uuaccaugaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8609
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8609
gggagaaagc uuaccaugaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccgac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8610
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8610
gggagaaagc uuaccaugag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8611
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8611
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8612
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8612
gggagaaagc uuaccauggg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8613
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8613
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8614
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8614
gggagaaagc uuaccaugga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccgacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  8615
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8615
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8616
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8616
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgccg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8617
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8617
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccgc cgugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8618
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8618
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcggcgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8619
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8619
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcgccgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8620
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8620
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8621
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8621
gggagaaagc uuaccaugau caacgccaga aaggagaaga agagaagagg cgccgacacc       60

agcgugggca ucgugggccu gcugcugacc accgccaugg ccgccgaggu gaccagaaga      120

ggcagcgccu acuacaugua ccuggacaga aacgacgccg gcgaggccau cagcuucccc      180

accacccugg gcaugaacaa gugcuacauc cagaucaugg accugggcca caugugcgac      240

gccaccauga gcuacgagug ccccaugcug gacgagggcg uggagcccga cgacguggac      300

ugcuggugca acaccaccag caccugggug guguacggca ccugccacca caagaagggc      360

gaggccagaa gaagcagaag agccgugacc cugcccagcc acagcaccag aaagcugcag      420

accagaagcc agaccuggcu ggagagcaga gaguacacca agcaccugau cagaguggag      480

aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg ccgccaucgc cuggcugcug      540

ggcagcagca ccagccagaa ggugaucuac cuggugauga uccugcugau cgcccccgcc      600

uacagcauca gaugcaucgg cgugagcaac agagacuucg uggagggcau gagcggcggc      660

accugggugg acaucgugcu ggagcacggc ggcugcguga ccgugauggc ccaggacaag      720

cccaccgugg acaucgagcu ggugaccacc accgugagca acauggccga ggugagaagc      780

uacugcuacg aggccagcau cagcgacaug gccagcgaca gcagaugccc cacccagggc      840

gaggccuacc uggacaagca gagcgacacc caguacgugu gcaagagaac ccugguggac      900

agaggcuggg gcaacggcug cggccuguuc ggcaagggca gccuggugac cugcgccaag      960

uucgccugca gcaagaagau gaccggcaag agcauccagc ccgagaaccu ggaguacaga     1020

aucaugcuga gcgugcacgg cagccagcac agcggcauga ucgugaacga caccggccac     1080

gagaccgacg agaacagagc caagguggag aucaccccca acagccccag agccgaggcc     1140

acccugggcg gcuucggcag ccugggccug gacugcgagc ccagaaccgg ccuggacuuc     1200

agcgaccugu acuaccugac caugaacaac aagcacuggc uggugcacaa ggagugguuc     1260

cacgacaucc cccugcccug gcacgccggc gccgacaccg gcacccccca cuggaacaac     1320

aaggaggccc ugguggaguu caaggacgcc cacgccaaga gacagaccgu gguggugcug     1380

ggcagccagg agggcgccgu gcacaccgcc cuggccggcg cccuggaggc cgagauggac     1440

ggcgccaagg gcagacugag cagcggccac cugaagugca gacugaagau ggacaagcug     1500

agacugaagg gcgugagcua cagccugugc accgccgccu ucaccuucac caagaucccc     1560

gccgagaccc ugcacggcac cgugaccgug gaggugcagu acgccggcac cgacggcccc     1620

ugcaaggugc ccgcccagau ggccguggac augcagaccc ugacccccgu gggcagacug     1680

aucaccgcca accccgugau caccgagagc accgagaaca gcaagaugau gcuggagcug     1740

gacccccccu ucggcgacag cuacaucgug aucggcgugg gcgagaagaa gaucacccac     1800

cacuggcaca gaagcggcag caccaucggc aaggccuucg aggccaccgu gagaggcgcc     1860

aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg gcagcguggg cggcgcccug     1920

aacagccugg gcaagggcau ccaccagauc uucggcgccg ccuucaagag ccuguucggc     1980

ggcaugagcu gguucagcca gauccugauc ggcacccugc ugauguggcu gggccugaac     2040

accaagaacg gcagcaucag ccugaugugc cuggcccugg gcggcgugcu gaucuuccug     2100

agcaccgccg ugagcgccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8622
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8622
gggagaaagc uuaccaugau caucaacgcc agaaaggaga agaagagaag aggcgccgac       60

accagcgugg gcaucguggg ccugcugcug accaccgcca uggccgccga ggugaccaga      120

agaggcagcg ccuacuacau guaccuggac agaaacgacg ccggcgaggc caucagcuuc      180

cccaccaccc ugggcaugaa caagugcuac auccagauca uggaccuggg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cuggacgagg gcguggagcc cgacgacgug      300

gacugcuggu gcaacaccac cagcaccugg gugguguacg gcaccugcca ccacaagaag      360

ggcgaggcca gaagaagcag aagagccgug acccugccca gccacagcac cagaaagcug      420

cagaccagaa gccagaccug gcuggagagc agagaguaca ccaagcaccu gaucagagug      480

gagaacugga ucuucagaaa ccccggcuuc gcccuggccg ccgccgccau cgccuggcug      540

cugggcagca gcaccagcca gaaggugauc uaccugguga ugauccugcu gaucgccccc      600

gccuacagca ucagaugcau cggcgugagc aacagagacu ucguggaggg caugagcggc      660

ggcaccuggg uggacaucgu gcuggagcac ggcggcugcg ugaccgugau ggcccaggac      720

aagcccaccg uggacaucga gcuggugacc accaccguga gcaacauggc cgaggugaga      780

agcuacugcu acgaggccag caucagcgac auggccagcg acagcagaug ccccacccag      840

ggcgaggccu accuggacaa gcagagcgac acccaguacg ugugcaagag aacccuggug      900

gacagaggcu ggggcaacgg cugcggccug uucggcaagg gcagccuggu gaccugcgcc      960

aaguucgccu gcagcaagaa gaugaccggc aagagcaucc agcccgagaa ccuggaguac     1020

agaaucaugc ugagcgugca cggcagccag cacagcggca ugaucgugaa cgacaccggc     1080

cacgagaccg acgagaacag agccaaggug gagaucaccc ccaacagccc cagagccgag     1140

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1200

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1260

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1320

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1380

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1440

gacggcgcca agggcagacu gagcagcggc caccugaagu gcagacugaa gauggacaag     1500

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaagauc     1560

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1620

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1680

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1740

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgagaa gaagaucacc     1800

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1860

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgcc     1920

cugaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1980

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcugaugug gcugggccug     2040

aacaccaaga acggcagcau cagccugaug ugccuggccc ugggcggcgu gcugaucuuc     2100

cugagcaccg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8623
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8623
gggagaaagc uuaccaugaa cgccagaaag gagaagaaga gaagaggcgc cgacaccagc       60

gugggcaucg ugggccugcu gcugaccacc gccauggccg ccgaggugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8624
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8624
gggagaaagc uuaccauggc cagaaaggag aagaagagaa gaggcgccga caccagcgug       60

ggcaucgugg gccugcugcu gaccaccgcc auggccgccg aggugaccag aagaggcagc      120

gccuacuaca uguaccugga cagaaacgac gccggcgagg ccaucagcuu ccccaccacc      180

cugggcauga acaagugcua cauccagauc auggaccugg gccacaugug cgacgccacc      240

augagcuacg agugccccau gcuggacgag ggcguggagc ccgacgacgu ggacugcugg      300

ugcaacacca ccagcaccug ggugguguac ggcaccugcc accacaagaa gggcgaggcc      360

agaagaagca gaagagccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      420

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      480

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      540

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      600

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      660

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      720

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      780

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      840

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      900

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      960

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug     1020

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc     1080

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     2100

gccgugagcg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8625
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8625
gggagaaagc uuaccaugag aaaggagaag aagagaagag gcgccgacac cagcgugggc       60

aucgugggcc ugcugcugac caccgccaug gccgccgagg ugaccagaag aggcagcgcc      120

uacuacaugu accuggacag aaacgacgcc ggcgaggcca ucagcuuccc caccacccug      180

ggcaugaaca agugcuacau ccagaucaug gaccugggcc acaugugcga cgccaccaug      240

agcuacgagu gccccaugcu ggacgagggc guggagcccg acgacgugga cugcuggugc      300

aacaccacca gcaccugggu gguguacggc accugccacc acaagaaggg cgaggccaga      360

agaagcagaa gagccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      420

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      480

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      540

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      600

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      660

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      720

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      780

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      840

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      900

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      960

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug     1020

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac     1080

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc     1140

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug     1200

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc     1260

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1320

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1380

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1440

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1500

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1560

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1620

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1680

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1740

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1800

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1860

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1920

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1980

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     2040

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     2100

gugagcgccu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8626
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8626
gggagaaagc uuaccaugaa ggagaagaag agaagaggcg ccgacaccag cgugggcauc       60

gugggccugc ugcugaccac cgccauggcc gccgagguga ccagaagagg cagcgccuac      120

uacauguacc uggacagaaa cgacgccggc gaggccauca gcuuccccac cacccugggc      180

augaacaagu gcuacaucca gaucauggac cugggccaca ugugcgacgc caccaugagc      240

uacgagugcc ccaugcugga cgagggcgug gagcccgacg acguggacug cuggugcaac      300

accaccagca ccuggguggu guacggcacc ugccaccaca agaagggcga ggccagaaga      360

agcagaagag ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      420

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      480

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      540

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      600

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      660

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      720

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      780

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      840

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      900

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      960

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc     1020

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag     1080

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     2100

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8627
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8627
gggagaaagc uuaccaugga gaagaagaga agaggcgccg acaccagcgu gggcaucgug       60

ggccugcugc ugaccaccgc cauggccgcc gaggugacca gaagaggcag cgccuacuac      120

auguaccugg acagaaacga cgccggcgag gccaucagcu uccccaccac ccugggcaug      180

aacaagugcu acauccagau cauggaccug ggccacaugu gcgacgccac caugagcuac      240

gagugcccca ugcuggacga gggcguggag cccgacgacg uggacugcug gugcaacacc      300

accagcaccu ggguggugua cggcaccugc caccacaaga agggcgaggc cagaagaagc      360

agaagagccg ugacccugcc cagccacagc accagaaagc ugcagaccag aagccagacc      420

uggcuggaga gcagagagua caccaagcac cugaucagag uggagaacug gaucuucaga      480

aaccccggcu ucgcccuggc cgccgccgcc aucgccuggc ugcugggcag cagcaccagc      540

cagaagguga ucuaccuggu gaugauccug cugaucgccc ccgccuacag caucagaugc      600

aucggcguga gcaacagaga cuucguggag ggcaugagcg gcggcaccug gguggacauc      660

gugcuggagc acggcggcug cgugaccgug auggcccagg acaagcccac cguggacauc      720

gagcugguga ccaccaccgu gagcaacaug gccgagguga gaagcuacug cuacgaggcc      780

agcaucagcg acauggccag cgacagcaga ugccccaccc agggcgaggc cuaccuggac      840

aagcagagcg acacccagua cgugugcaag agaacccugg uggacagagg cuggggcaac      900

ggcugcggcc uguucggcaa gggcagccug gugaccugcg ccaaguucgc cugcagcaag      960

aagaugaccg gcaagagcau ccagcccgag aaccuggagu acagaaucau gcugagcgug     1020

cacggcagcc agcacagcgg caugaucgug aacgacaccg gccacgagac cgacgagaac     1080

agagccaagg uggagaucac ccccaacagc cccagagccg aggccacccu gggcggcuuc     1140

ggcagccugg gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac     1200

cugaccauga acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug     1260

cccuggcacg ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug     1320

gaguucaagg acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc     1380

gccgugcaca ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga     1440

cugagcagcg gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug     1500

agcuacagcc ugugcaccgc cgccuucacc uucaccaaga uccccgccga gacccugcac     1560

ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa ggugcccgcc     1620

cagauggccg uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc     1680

gugaucaccg agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc     1740

gacagcuaca ucgugaucgg cgugggcgag aagaagauca cccaccacug gcacagaagc     1800

ggcagcacca ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug     1860

cugggcgaca ccgccuggga cuucggcagc gugggcggcg cccugaacag ccugggcaag     1920

ggcauccacc agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc     1980

agccagaucc ugaucggcac ccugcugaug uggcugggcc ugaacaccaa gaacggcagc     2040

aucagccuga ugugccuggc ccugggcggc gugcugaucu uccugagcac cgccgugagc     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8628
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8628
gggagaaagc uuaccaugaa gaagagaaga ggcgccgaca ccagcguggg caucgugggc       60

cugcugcuga ccaccgccau ggccgccgag gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8629
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8629
gggagaaagc uuaccaugaa gagaagaggc gccgacacca gcgugggcau cgugggccug       60

cugcugacca ccgccauggc cgccgaggug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8630
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8630
gggagaaagc uuaccaugag aagaggcgcc gacaccagcg ugggcaucgu gggccugcug       60

cugaccaccg ccauggccgc cgaggugacc agaagaggca gcgccuacua cauguaccug      120

gacagaaacg acgccggcga ggccaucagc uuccccacca cccugggcau gaacaagugc      180

uacauccaga ucauggaccu gggccacaug ugcgacgcca ccaugagcua cgagugcccc      240

augcuggacg agggcgugga gcccgacgac guggacugcu ggugcaacac caccagcacc      300

uggguggugu acggcaccug ccaccacaag aagggcgagg ccagaagaag cagaagagcc      360

gugacccugc ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag      420

agcagagagu acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc      480

uucgcccugg ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug      540

aucuaccugg ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug      600

agcaacagag acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag      660

cacggcggcu gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug      720

accaccaccg ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc      780

gacauggcca gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc      840

gacacccagu acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc      900

cuguucggca agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc      960

ggcaagagca uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc     1020

cagcacagcg gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag     1080

guggagauca cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug     1140

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug     1200

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac     1260

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1320

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1380

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc     1440

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1500

cugugcaccg ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug     1560

accguggagg ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc     1620

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1680

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1740

aucgugaucg gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc     1800

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1860

accgccuggg acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac     1920

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1980

cugaucggca cccugcugau guggcugggc cugaacacca agaacggcag caucagccug     2040

augugccugg cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8631
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8631
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccga ggugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8632
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8632
gggagaaagc uuaccauggg cgccgacacc agcgugggca ucgugggccu gcugcugacc       60

accgccaugg ccgccgaggu gaccagaaga ggcagcgccu acuacaugua ccuggacaga      120

aacgacgccg gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cuggccgccg ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag     1080

aucaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc     2040

cuggcccugg gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8633
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8633
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ugggccugcu gcugaccacc       60

gccauggccg ccgaggugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  8634
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8634
gggagaaagc uuaccaugga caccagcgug ggcaucgugg gccugcugcu gaccaccgcc       60

auggccgccg aggugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg ccugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8635
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  8635
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8636
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8636
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgccg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8637
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8637
gggagaaagc uuaccaugag aggcgccgac accagcgugg gcaucguggg ccugcugcug       60

accaccgcca uggccgccgc cgugaccaga agaggcagcg ccuacuacau guaccuggac      120

agaaacgacg ccggcgaggc caucagcuuc cccaccaccc ugggcaugaa caagugcuac      180

auccagauca uggaccuggg ccacaugugc gacgccacca ugagcuacga gugccccaug      240

cuggacgagg gcguggagcc cgacgacgug gacugcuggu gcaacaccac cagcaccugg      300

gugguguacg gcaccugcca ccacaagaag ggcgaggcca gaagaagcag aagagccgug      360

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      420

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      480

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      540

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      600

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      660

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      720

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      780

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      840

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      900

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      960

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag     1020

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug     1080

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc     1140

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac     1200

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc     1260

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1320

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1380

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1440

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1500

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1560

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1620

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1680

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1740

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1800

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1860

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1920

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1980

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     2040

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8638
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8638
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcggcgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8639
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8639
gggagaaagc uuaccaugag aggccugagc agcagaaaga gaagaagcca cgacgugcug       60

accgugcagu uccugauccu gggcaugcug cugaugaccg gcgccgugac cagaagaggc      120

agcgccuacu acauguaccu ggacagaaac gacgccggcg aggccaucag cuuccccacc      180

acccugggca ugaacaagug cuacauccag aucauggacc ugggccacau gugcgacgcc      240

accaugagcu acgagugccc caugcuggac gagggcgugg agcccgacga cguggacugc      300

uggugcaaca ccaccagcac cuggguggug uacggcaccu gccaccacaa gaagggcgag      360

gccagaagaa gcagaagagc cgugacccug cccagccaca gcaccagaaa gcugcagacc      420

agaagccaga ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac      480

uggaucuuca gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc      540

agcagcacca gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac      600

agcaucagau gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc      660

uggguggaca ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc      720

accguggaca ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac      780

ugcuacgagg ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag      840

gccuaccugg acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga      900

ggcuggggca acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc      960

gccugcagca agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc     1020

augcugagcg ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag     1080

accgacgaga acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc     1140

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc     1200

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1320

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1380

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1440

gccaagggca gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga     1500

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc     1560

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1620

aaggugcccg cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1680

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1740

ccccccuucg gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac     1800

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1860

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac     1920

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1980

augagcuggu ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc     2040

aagaacggca gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc     2100

accgccguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8640
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 3 opt

<400>  8640
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8641
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 3 opt

<400>  8641
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8642
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 3 opt

<400>  8642
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1080

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8643
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 3 opt

<400>  8643
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8644
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 3 opt

<400>  8644
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8645
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 3 opt

<400>  8645
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucacccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8646
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 3 opt

<400>  8646
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccacagaaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8647
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 3 opt

<400>  8647
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaccgu gaacgacauc     1080

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8648
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 3 opt

<400>  8648
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1080

gacgagaaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1140

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1200

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1260

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1320

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1380

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1440

aagggcaagc uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1500

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1560

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1620

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1680

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1740

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1800

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1860

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1920

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1980

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     2040

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2100

gccgugagcg ccgacguggg cugcagcgug gacuucagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8649
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 3 opt

<400>  8649
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcag aggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8650
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 3 opt

<400>  8650
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacgccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8651
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 3 opt

<400>  8651
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc cuggcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8652
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 3 opt

<400>  8652
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu gguacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8653
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 3 opt

<400>  8653
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgagauc      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8654
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 3 opt

<400>  8654
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcuggc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8655
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 3 opt

<400>  8655
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc acccuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8656
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 3 opt

<400>  8656
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccguggugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8657
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 3 opt

<400>  8657
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcgcc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaccccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8658
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 3 opt

<400>  8658
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag caagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8659
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 3 opt

<400>  8659
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag acagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8660
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 3 opt

<400>  8660
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaagcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaccc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8661
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 3 opt

<400>  8661
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccaacg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccagaa gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8662
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 3 opt

<400>  8662
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gagaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8663
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 3 opt

<400>  8663
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gccugagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8664
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 3 opt

<400>  8664
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccaacg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggauguucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8665
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 3 opt

<400>  8665
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggauccugag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcaucggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8666
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 3 opt

<400>  8666
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug agcaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc caccaucagc gacaucgcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccgccgug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8667
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 3 opt

<400>  8667
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca cccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8668
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 3 opt

<400>  8668
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagc ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8669
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 3 opt

<400>  8669
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gagaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

aucgagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8670
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 3 opt

<400>  8670
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaucg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8671
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 3 opt

<400>  8671
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ugaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagcuuc augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8672
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 3 opt

<400>  8672
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcaa gcugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8673
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 3 opt

<400>  8673
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gaggcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8674
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 3 opt

<400>  8674
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcgccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8675
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 3 opt

<400>  8675
gggagaaagc uuaccaugag aaucaucaac gccggcaagg agaagaagag aagaggcgcc       60

gacaccaacg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccagaa gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8676
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 3 opt

<400>  8676
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gagaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaagg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8677
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 3 opt

<400>  8677
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacaugguga gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8678
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 3 opt

<400>  8678
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccaacg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacau gaccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8679
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 3 opt

<400>  8679
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccgccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagacc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8680
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 3 opt

<400>  8680
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gagaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cuggccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8681
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 3 opt

<400>  8681
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcauc agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcauccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8682
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 3 opt

<400>  8682
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caucagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8683
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 3 opt

<400>  8683
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg cccagagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8684
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 3 opt

<400>  8684
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcaggcca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8685
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 3 opt

<400>  8685
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8686
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 3 opt

<400>  8686
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac accggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagggcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gugcugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8687
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 3 opt

<400>  8687
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgc cauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugcuggu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggc ccacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg ccaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agaccgugga cggcaccgug accguggagg gccaguacgg cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucaucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cggcggcgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8688
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 3 opt

<400>  8688
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucagcaag     1560

auccccgccg agacccugca cggcaccgug accgaggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8689
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 3 opt

<400>  8689
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8690
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 3 opt

<400>  8690
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca acgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca ccaugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8691
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 3 opt

<400>  8691
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     2040

cugaacacca agaacggcag caucagccug gugugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8692
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 3 opt

<400>  8692
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcacc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagcccg ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8693
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 3 opt

<400>  8693
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgu gaucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8694
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 3 opt

<400>  8694
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcga ggccaucagc      180

uuccccacca cccugggcgu gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu gguacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8695
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 3 opt

<400>  8695
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agaagaagag aagaggcgcc       60

gacaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgu ggaggugacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uuccccacca cccugggcgu gaacaagugc uacauccaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugcugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8696
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 3 opt

<400>  8696
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucacccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8697
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 3 opt

<400>  8697
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca accugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcgugcac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8698
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 3 opt

<400>  8698
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8699
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 3 opt

<400>  8699
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca accugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8700
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 3 opt

<400>  8700
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8701
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 3 opt

<400>  8701
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccagcacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgugugcac cgccgccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8702
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 3 opt

<400>  8702
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacgccagca ucggcaucgu gagccugcug cugaccaccg ugauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgugacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acagcggcgc cgacaccgag accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcagag acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ucaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8703
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 3 opt

<400>  8703
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucgu gggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      540

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag     1080

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc     1140

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac     1200

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc     1260

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1320

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1380

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1440

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1500

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1560

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1620

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1680

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1740

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1800

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1860

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcguguucaa cagccugggc     1920

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1980

uucagccaga uccugaucgg cacccugcug guguggcugg gccugaacac caagaacggc     2040

agcaucagcc ugaccugccu ggcccugggc ggcgugauga ucuuccugag caccgccgug     2100

agcgccgacg ugggcugcag cguggacuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8704
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 3 opt

<400>  8704
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgaga ccagaagaag cagaagaagc gugagccuga gauaccacua caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaagaagca ccugaucaug      480

guggagaacu ggaucuucag aaaccccggc uucgccaucg ugagcguggc caucaccugg      540

cugaugggca gccugaccag ccagaaggug aucuaccugg ugaugaucgu gcugaucgug      600

cccgccuaca gcaucagcug caucggcgug agcaacagag accuggugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccga gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaugaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagccugagc gacauggcca gcgccagcag augccccacc      840

cagggcgagc ccagccugga caagcagagc gacacccaga gcgugugcaa gagaacccug      900

ggcgacagag gcuggggcaa cggcugcggc aucuucggca agggcagccu ggugaccugc      960

agcaaguuca ccugcugcaa gaagaugccc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugcccgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcagagcgc cggcaccgac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug accugccugg cccugggcgg cgugaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8705
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 3 opt

<400>  8705
gggagaaagc uuaccaugag aaucaucaac gccagaaagg agagaaagag aagaggcgcc       60

gacaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgagaucacc      120

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      180

uucgccacca ccaugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      240

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      300

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      360

aagggcgagg ccagacccag cagaagagcc gugacccugc ccagccacag caccagaaag      420

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      480

guggagaacu ggauccugag aaaccccggc uucaccgugg ccgcccuggc cauggccugg      540

cugcugggca acagcaccag ccagagagug aucuaccugg ugaugauccu gcugaucgcc      600

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      660

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      720

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      780

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      840

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      900

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      960

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag     1020

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc     1080

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc     1140

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1200

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1260

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1320

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1380

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1440

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagaau caagauggac     1500

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucagcaag     1560

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcagagac     1620

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1680

agacugauca ccgccaaccc caucaucacc gagagcaccg agaacagcag aaugaugcug     1740

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1800

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1860

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1920

guguucaaca gccugggcaa gggcgugcac cagguguucg gcgccgccuu caagagccug     1980

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcuggu guggcugggc     2040

cugaacacca agaacggcag caucagccug gccugccugg cccugggcgg cgccaugauc     2100

uuccugagca ccgccgugag cgccgacgug ggcugcagcg uggacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  8706
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 3 opt

<400>  8706
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8707
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 3 opt

<400>  8707
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8708
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 3 opt

<400>  8708
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8709
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 3 opt

<400>  8709
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8710
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 3 opt

<400>  8710
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcua cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcaagcugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaagauc cccgugcaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8711
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 3 opt

<400>  8711
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccaca gaaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcua cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcaagcugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaagauc cccgugcaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8712
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 3 opt

<400>  8712
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

accgugaacg acaucggcua cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcaagcugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaagauc cccgugcaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8713
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 3 opt

<400>  8713
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucac ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8714
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 3 opt

<400>  8714
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga gaacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg caagcuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8715
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 3 opt

<400>  8715
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcagaggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8716
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 3 opt

<400>  8716
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggccuggc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8717
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 3 opt

<400>  8717
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacugguac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8718
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 3 opt

<400>  8718
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga cacccagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8719
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 3 opt

<400>  8719
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg agaucagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8720
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 3 opt

<400>  8720
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cuggcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8721
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 3 opt

<400>  8721
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccacccu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8722
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 3 opt

<400>  8722
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgug gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8723
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 3 opt

<400>  8723
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcgccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaccc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8724
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 3 opt

<400>  8724
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagcaaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8725
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 3 opt

<400>  8725
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagacaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8726
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 3 opt

<400>  8726
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcacccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8727
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 3 opt

<400>  8727
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagccug agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8728
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 3 opt

<400>  8728
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagaaucga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8729
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 3 opt

<400>  8729
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc cugagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcauc     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8730
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 3 opt

<400>  8730
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugagcac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccacca ucagcgacau cgccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ccguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8731
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 3 opt

<400>  8731
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggagcugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8732
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 3 opt

<400>  8732
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caucgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8733
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 3 opt

<400>  8733
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgugaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gcuucaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8734
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 3 opt

<400>  8734
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcaagcuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8735
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 3 opt

<400>  8735
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaaggacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8736
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 3 opt

<400>  8736
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggaggc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8737
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 3 opt

<400>  8737
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cgcccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agacccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8738
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 3 opt

<400>  8738
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggcg ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8739
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 3 opt

<400>  8739
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccuggc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8740
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 3 opt

<400>  8740
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggugagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8741
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 3 opt

<400>  8741
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcaucagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca uccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8742
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 3 opt

<400>  8742
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcauca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8743
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 3 opt

<400>  8743
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggcccag agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8744
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 3 opt

<400>  8744
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca ggccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8745
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 3 opt

<400>  8745
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca gcaagauccc cgccgagacc cugcacggca ccgugaccga ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8746
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 3 opt

<400>  8746
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacaccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgaggg caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgugcu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8747
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 3 opt

<400>  8747
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8748
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 3 opt

<400>  8748
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcaacgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccaccaug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8749
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 3 opt

<400>  8749
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccuggugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8750
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 3 opt

<400>  8750
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugaacaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccuggugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8751
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 3 opt

<400>  8751
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccgccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8752
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 3 opt

<400>  8752
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgugaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8753
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 3 opt

<400>  8753
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcgugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacugguac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8754
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 3 opt

<400>  8754
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccguggagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuuccc caccacccug ggcgugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8755
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 3 opt

<400>  8755
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucac ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8756
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 3 opt

<400>  8756
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccaaccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggcg ugcaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8757
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 3 opt

<400>  8757
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8758
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 3 opt

<400>  8758
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccaaccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8759
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 3 opt

<400>  8759
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8760
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 3 opt

<400>  8760
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccagc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgug     1500

ugcaccgccg ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8761
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 3 opt

<400>  8761
gggagaaagc uuaccaugac cagcaucggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acgagaacag agccaaggug gaggugaccc ccaacagccc cagagccgag     1080

gccacccugg gcggcuucgg cagccugggc cuggacugcg agcccagaac cggccuggac     1140

uucagcgacc uguacuaccu gaccaugaac aacaagcacu ggcuggugca caaggagugg     1200

uuccacgaca ucccccugcc cuggcacgcc ggcgccgaca ccggcacccc ccacuggaac     1260

aacaaggagg cccuggugga guucaaggac gcccacgcca agagacagac cgugguggug     1320

cugggcagcc aggagggcgc cgugcacacc gcccuggccg gcgcccugga ggccgagaug     1380

gacggcgcca agggcagacu guucagcggc caccugaagu gcagacugaa gauggacaag     1440

cugagacuga agggcgugag cuacagccug ugcaccgccg ccuucaccuu caccaaggug     1500

cccgccgaga cccugcacgg caccgugacc guggaggugc aguacgccgg caccgacggc     1560

cccugcaagg ugcccgccca gauggccgug gacaugcaga cccugacccc cgugggcaga     1620

cugaucaccg ccaaccccgu gaucaccgag agcaccgaga acagcaagau gaugcuggag     1680

cuggaccccc ccuucggcga cagcuacauc gugaucggcg ugggcgacaa gaagaucacc     1740

caccacuggc acagaagcgg cagcaccauc ggcaaggccu ucgaggccac cgugagaggc     1800

gccaagagaa uggccgugcu gggcgacacc gccugggacu ucggcagcgu gggcggcgug     1860

uucaacagcc ugggcaaggg cauccaccag aucuucggcg ccgccuucaa gagccuguuc     1920

ggcggcauga gcugguucag ccagauccug aucggcaccc ugcuggugug gcugggccug     1980

aacaccaaga acggcagcau cagccugacc ugccuggccc ugggcggcgu gaugaucuuc     2040

cugagcaccg ccgugagcgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8762
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 3 opt

<400>  8762
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccgaga ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgagaccaga agaagcagaa gaagcgugag ccugagauac      360

cacuacacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacaag      420

aagcaccuga ucauggugga gaacuggauc uucagaaacc ccggcuucgc caucgugagc      480

guggccauca ccuggcugau gggcagccug accagccaga aggugaucua ccuggugaug      540

aucgugcuga ucgugcccgc cuacagcauc agcugcaucg gcgugagcaa cagagaccug      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgagaugg cccaggacaa gcccaccgug gacaucgagc uggugaccau gaccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagcc ugagcgacau ggccagcgcc      780

agcagaugcc ccacccaggg cgagcccagc cuggacaagc agagcgacac ccagagcgug      840

ugcaagagaa cccugggcga cagaggcugg ggcaacggcu gcggcaucuu cggcaagggc      900

agccugguga ccugcagcaa guucaccugc ugcaagaaga ugcccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug cccgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaucggcca cgagaccgac gagaacagag ccaaggugga ggugaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

agcgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     2040

ggcggcguga ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8763
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 3 opt

<400>  8763
gggagaaagc uuaccaugag cgugggcauc gugggccugc ugcugaccac cgccauggcc       60

gccgagguga ccagaagagg cagcgccuac uacauguacc uggacagaaa cgacgccggc      120

gaggccauca gcuuccccac cacccugggc augaacaagu gcuacaucca gaucauggac      180

cugggccaca ugugcgacgc caccaugagc uacgagugcc ccaugcugga cgagggcgug      240

gagcccgacg acguggacug cuggugcaac accaccagca ccuggguggu guacggcacc      300

ugccaccaca agaagggcga ggccagaaga agcagaagag ccgugacccu gcccagccac      360

agcaccagaa agcugcagac cagaagccag accuggcugg agagcagaga guacaccaag      420

caccugauca gaguggagaa cuggaucuuc agaaaccccg gcuucgcccu ggccgccgcc      480

gccaucgccu ggcugcuggg cagcagcacc agccagaagg ugaucuaccu ggugaugauc      540

cugcugaucg cccccgccua cagcaucaga ugcaucggcg ugagcaacag agacuucgug      600

gagggcauga gcggcggcac cuggguggac guggugcugg agcacggcgg cugcgugacc      660

gugauggccc aggacaagcc caccguggac aucgagcugg ugaccaccac cgugagcaac      720

auggccgagg ugagaagcua cugcuacgag gccagcauca gcgacauggc cagcgacagc      780

agaugcccca cccagggcga ggccuaccug gacaagcaga gcgacaccca guacgugugc      840

aagagaaccc ugguggacag aggcuggggc aacggcugcg gccuguucgg caagggcagc      900

cuggugaccu gcgccaaguu cgccugcagc aagaagauga ccggcaagag cauccagccc      960

gagaaccugg aguacagaau caugcugagc gugcacggca gccagcacag cggcaugauc     1020

gugaacgaca ccggccacga gaccgacgag aacagagcca agguggagau cacccccaac     1080

agccccagag ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc     1140

agaaccggcc uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug     1200

gugcacaagg agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc     1260

accccccacu ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga     1320

cagaccgugg uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc     1380

cuggaggccg agauggacgg cgccaagggc agacugagca gcggccaccu gaagugcaga     1440

cugaagaugg acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc     1500

accuucacca agauccccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac     1560

gccggcaccg acggccccug caaggugccc gcccagaugg ccguggacau gcagacccug     1620

acccccgugg gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc     1680

aagaugaugc uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc     1740

gagaagaaga ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag     1800

gccaccguga gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc     1860

agcgugggcg gcgcccugaa cagccugggc aagggcaucc accagaucuu cggcgccgcc     1920

uucaagagcc uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug     1980

auguggcugg gccugaacac caagaacggc agcaucagcc ugaugugccu ggcccugggc     2040

ggcgugcuga ucuuccugag caccgccgug agcgccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  8764
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 3 opt

<400>  8764
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc cagaagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8765
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 3 opt

<400>  8765
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggaug uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8766
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 3 opt

<400>  8766
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaugacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8767
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 3 opt

<400>  8767
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccgagg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgccaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

cuggugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggcccaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgccacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc guggacggca ccgugaccgu ggagggccag     1560

uacggcggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau caucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8768
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 3 opt

<400>  8768
gggagaaagc uuaccauggg cgccgacgcc agcaucggca ucgugagccu gcugcugacc       60

accgugaugg ccgccgagau caccagaaga ggcagcgccu acuacaugua ccuggacaga      120

agcgacgccg gcaaggccau cagcuucgug accacccugg gcgugaacaa gugccacgug      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau caagguggag aacuggaucu ucagaaaccc cggcuucgcc      480

cugguggccg uggccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucaccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga cgagaacaga gccaaggugg aggugacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacagcg gcgccgacac cgagaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug agcagcggcc accugaagug cagacugaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

agagacggcc ccugcaaggu gcccgcccag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     1740

aagaucaccc accacuggca cagaagcggc agcaucaucg gcaaggccuu cgaggccacc     1800

gugagaggcg ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgugu ucaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcuggugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaccu gccuggcccu gggcggcgug     2040

augaucuucc ugagcaccgc cgugagcgcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  8769
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 3 opt

<400>  8769
gggagaaagc uuaccauggg cgccgacacc agcaucggca ucaucggccu gcugcugacc       60

accgccaugg ccgccgagau caccagaaga ggcagcgccu acuacaugua ccuggacaga      120

agcgacgccg gcaaggccau cagcuucgcc accaccaugg gcgugaacaa gugccacgug      180

cagaucaugg accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug      240

gacgagggcg uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug      300

guguacggca ccugccacca caagaagggc gaggccagac ccagcagaag agccgugacc      360

cugcccagcc acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga      420

gaguacacca agcaccugau caagguggag aacuggaucc ugagaaaccc cggcuucacc      480

guggccgccc uggccauggc cuggcugcug ggcaacagca ccagccagag agugaucuac      540

cuggugauga uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac      600

agagacuucg uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc      660

ggcugcguga ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc      720

accgugagca acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug      780

gccagcgaca gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc      840

caguacgugu gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc      900

ggcaagggca gccuggugac cugcgccaag uucaccugca gcaagaagau gaccggcaag      960

agcauccagc ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac     1020

agcggcauga ucgugaacga caucggccac gagaccgacg agaacagagc caagguggag     1080

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac     1440

cugaagugca gaaucaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucag caaggugccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcag agacggcccc ugcaaggugc ccgcccagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccaucau caccgagagc     1680

accgagaaca gcagaaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcguguuc aacagccugg gcaagggcgu gcaccaggug     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugguguggcu gggccugaac accaagaacg gcagcaucag ccuggccugc     2040

cuggcccugg gcggcgccau gaucuuccug agcaccgccg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8770
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 3 opt

<400>  8770
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8771
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 3 opt

<400>  8771
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8772
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 3 opt

<400>  8772
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      540

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug      600

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc      840

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      900

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug      960

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1140

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1320

auggccgugc ugggcgacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8773
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 3 opt

<400>  8773
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8774
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 3 opt

<400>  8774
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8775
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 3 opt

<400>  8775
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaccgu gaacgacauc      540

ggcuacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcaa gcuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca agauccccgu gcagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8776
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 3 opt

<400>  8776
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacuguuca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agcugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  8777
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 3 opt

<400>  8777
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      540

gacgagaaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug      600

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      660

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      720

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag      780

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc      840

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc      900

aagggcaagc uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug      960

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1020

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1080

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1140

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1200

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1320

auggccgugc ugggcgacac cgccugggac uucggcagcu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8778
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 3 opt

<400>  8778
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gccugagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8779
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 3 opt

<400>  8779
gggagaaagc uuaccaugag caagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8780
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 3 opt

<400>  8780
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu gguacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8781
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 3 opt

<400>  8781
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcaa gcugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8782
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 3 opt

<400>  8782
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaccc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8783
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 3 opt

<400>  8783
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgagauc      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8784
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 3 opt

<400>  8784
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccagaa gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8785
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 3 opt

<400>  8785
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacaugguga gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8786
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 3 opt

<400>  8786
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaucg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8787
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 3 opt

<400>  8787
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcaucggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8788
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 3 opt

<400>  8788
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cuggccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8789
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 3 opt

<400>  8789
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca cccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8790
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 3 opt

<400>  8790
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcuggc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8791
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 3 opt

<400>  8791
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gaggcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8792
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 3 opt

<400>  8792
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccgccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8793
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 3 opt

<400>  8793
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug agcaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc caccaucagc gacaucgcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccgccgug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8794
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 3 opt

<400>  8794
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucagcaag     1020

auccccgccg agacccugca cggcaccgug accgaggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8795
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 3 opt

<400>  8795
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagc ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8796
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 3 opt

<400>  8796
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcauc agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcauccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8797
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 3 opt

<400>  8797
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ugaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8798
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 3 opt

<400>  8798
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcgccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8799
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 3 opt

<400>  8799
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac accggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagggcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8800
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 3 opt

<400>  8800
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgc cauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugcuggu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggc ccacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg ccaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agaccgugga cggcaccgug accguggagg gccaguacgg cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8801
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 3 opt

<400>  8801
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagcccg ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8802
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 3 opt

<400>  8802
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug aacaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8803
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 3 opt

<400>  8803
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcgccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8804
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 3 opt

<400>  8804
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8805
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 3 opt

<400>  8805
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu gguacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8806
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 3 opt

<400>  8806
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8807
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 3 opt

<400>  8807
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8808
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 3 opt

<400>  8808
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgugugcac cgccgccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8809
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 3 opt

<400>  8809
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacgag      540

aacagagcca agguggaggu gacccccaac agccccagag ccgaggccac ccugggcggc      600

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      660

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      720

cugcccuggc acagcggcgc cgacaccgag accccccacu ggaacaacaa ggaggcccug      780

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag      840

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc      900

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc      960

gugagcuaca gccugugcac cgccgccuuc accuucacca aggugcccgc cgagacccug     1020

cacggcaccg ugaccgugga ggugcaguac gccggcagag acggccccug caaggugccc     1080

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1140

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1200

ggcgacagcu acaucgugau cggcgugggc gacaagaaga ucacccacca cuggcacaga     1260

agcggcagca ucaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1320

gugcugggcg acaccgccug ggacuucggc agcugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  8810
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 3 opt

<400>  8810
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ugaugaucgu gcugaucgug       60

cccgccuaca gcaucagcug caucggcgug agcaacagag accuggugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccga gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaugaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagccugagc gacauggcca gcgccagcag augccccacc      300

cagggcgagc ccagccugga caagcagagc gacacccaga gcgugugcaa gagaacccug      360

ggcgacagag gcuggggcaa cggcugcggc aucuucggca agggcagccu ggugaccugc      420

agcaaguuca ccugcugcaa gaagaugccc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugcccgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcagagcgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8811
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 3 opt

<400>  8811
gggagaaagc uuaccaugag acagaaggug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      540

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1020

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8812
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 3 opt

<400>  8812
gggagaaagc uuaccaugag ccagagagug aucuaccugg ugaugauccu gcugaucgcc       60

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      120

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      180

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      240

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      300

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      360

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      420

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      480

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacauc      540

ggccacgaga ccgacgagaa cagagccaag guggagguga cccccaacag ccccagagcc      600

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      660

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      720

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg      780

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug      840

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag      900

auggacggcg ccaagggcag acuguucagc ggccaccuga agugcagaau caagauggac      960

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucagcaag     1020

gugcccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcagagac     1080

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1140

agacugauca ccgccaaccc caucaucacc gagagcaccg agaacagcag aaugaugcug     1200

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga caagaagauc     1260

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1320

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8813
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  8813
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8814
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 3 opt

<400>  8814
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaca      240

ugugacgcca ccaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacacuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaucucagac cuggcucgag agccgggaau acacgaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacauc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcucag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacc      840

caaggagaag ccuaccugga caaacagucu gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcuaaguuug cgugcuccaa gaaaaugacc ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauacg     1080

ggacaugaga cagacgagaa uagagccaaa guggaaauua ccccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auacaggaac gccccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acagcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacgcugca uggcaccgug acaguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cggcgaaccc cguuaucaca gaauccaccg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac acagccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaaugcca agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca ccgccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8815
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 3 opt

<400>  8815
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgg auacgaaacg     1080

gaugaggaca gagcuaaggu ggaggucaca ccuaauagcc cgcgcgcgga agccacccuu     1140

ggcggcuucg ggucccuggg acucgauugu gagccccgga ccggccugga cuuuucugau     1200

cuuuauuacc ugacaaugaa caauaaacac uggcucgugc auaaggaaug guuccacgac     1260

auuccucugc ccuggcacgc uggggccgau accggaacac cucauuggaa caauaaagag     1320

gcccuggucg aauuuaagga cgcucacgcc aagagacaga cggugguugu gcucggcagc     1380

caggaggggg cgguccauac cgcucuggcc ggcgcccuug aggcugaaau ggauggagcc     1440

aaaggccgcc uguucuccgg gcaccucaag ugccggcuga aaauggacaa acuuagacug     1500

aagggaguga gcuauucucu cuguacagcu gccuuuaccu ucacaaaggu ucccgcggag     1560

acccugcaug gcacggugac cgucgaagug caguacgccg ggacagacgg accgugcaag     1620

aucccugugc aaauggcugu ugauaugcag acccugacac ccgucggccg ccucaucacc     1680

gccaacccug ugauuacgga guccacagaa aauagcaaaa ugaugcugga gcuugacccu     1740

cccuuugggg auucuuacau cgugauuggc gucggagaca agaaaaucac ccaccacugg     1800

caucgguccg gcagcacaau ugggaaggcu uucgaggcca ccgugagagg agcgaaacgc     1860

auggccguuc ugggcgauac cgcuugggac uucggguccg ugggaggcgu cuuuaacucu     1920

cucgggaagg gcauccacca gaucuucgga gccgcuuuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauucu gaucggaaca cuucucgugu ggcugggccu gaacacgaaa     2040

aaugggagca uuucccucac cugucuggcc cuuggaggcg uuaugaucuu ccugagcaca     2100

gccgugucug cugaugucgg gugcuccgug gacuuuagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8816
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 3 opt

<400>  8816
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8817
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  8817
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8818
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 3 opt

<400>  8818
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccguug ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa cgcuaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu gagcaccgcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8819
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 3 opt

<400>  8819
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucggguacg agacggacga agauagagcc aagguggagg ucacaccuaa cagcccgcgg     1080

gccgaggcua cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga     1140

cuggauuuuu cugaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag     1200

gagugguucc acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau     1260

uggaauaaca aagaagcucu ggucgaguuu aaggaugccc acgccaaacg ccagacggug     1320

guugugcucg gaagccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggacg gcgccaaggg acggcuguuc uccggccacc ucaagugcag acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucucucugua cagcugccuu uaccuucaca     1500

aagguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gacgggccgu gcaaaauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacggcuca uuaccgccaa uccugugauc acggaaucca cagagaacag caagaugaug     1680

cuggaacuug auccucccuu uggcgacucu uacaucguga uuggggucgg cgauaagaaa     1740

aucacccacc acuggcauag auccggaagc acaauuggca aggcuuucga ggccaccgug     1800

cgcggggcua aacggauggc cguucuggga gacaccgcgu gggauuucgg cuccgucggg     1860

ggaguguuua auucucucgg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc     1920

cuguucggag gcauguccug guuuucucaa auccugaucg ggacacuucu cguguggcug     1980

ggacugaaca cgaagaacgg cagcauuucc cucaccuguc uggcccuugg gggaguuaug     2040

aucuuccuga gcacagcugu gucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8820
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 3 opt

<400>  8820
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8821
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 3 opt

<400>  8821
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggauau uguccuugaa cacgggggau gugugacagu uauggcccaa      180

gacaaaccua cgguggauau cgagcugguc accacaaccg uguccaauau ggcggaaguu      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu gcaugggucu cagcacuccg gaaugauugu caacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guugaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu ggaguucaag gaugcucacg ccaagcggca gacagucgug      840

guucucggga gccaggaagg agcggugcau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auccccgcgg agacccuuca cgggacagug accguugaag ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauua cagccaaucc cgugaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucguuauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8822
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 3 opt

<400>  8822
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8823
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 3 opt

<400>  8823
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgg cuaugaaaca      540

gaugaggacc gggccaaggu ugaagugacc ccuaacagcc cccgcgcuga ggccacacuc      600

gggggauuug gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau      660

cuguacuacc ucacgaugaa uaacaaacau uggcuggugc acaaggaaug guuucaugac      720

auuccccuuc cuuggcacgc gggagccgac acaggcaccc cgcacuggaa uaacaaagag      780

gcucuggucg aguucaagga ugcccaugcu aagcggcaga cagugguugu gcucgggagc      840

caggaaggag ccguccacac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu      900

aaaggacgcc ucuuuuccgg ccaucugaag uguagacuua aaauggauaa gcugcggcuc      960

aaaggcgugu cuuauagccu gugcaccgcc gccuucacau uuacgaaggu ucccgcugag     1020

acccuucacg ggacagugac cgucgaagug caauacgcgg gaacagacgg cccuuguaaa     1080

auccccgucc agauggccgu ggauaugcag acccugacgc cuguugggcg ccucauuaca     1140

gcuaaucccg ugaucaccga guccaccgaa aacagcaaga ugaugcugga gcuggacccg     1200

ccuuucggag auucuuauau ugucaucggc gugggggaca agaaaaucac acaccauugg     1260

cacagauccg gcagcaccau uggaaaggcc uuugaagcca caguucgggg cgcuaaacgc     1320

auggccgugc ucggggauac ggcgugggac uucggaucuu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  8824
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 3 opt

<400>  8824
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  8825
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       3 opt

<400>  8825
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcugag cggaaagggc      900

ucccugguga ccugcgccaa auucgccugc agcaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug uccguccacg gaagccaaca cucuggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccg     1080

aauagcccca gagcggaagc cacccuugga ggcuuuggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu cucugaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ucacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgagu ucaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaagccag gaaggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugucuu auucccucug uacagcugcc     1500

uuuaccuuca caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uuuggcgacu ccuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcagcggau ccacaauugg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuu     1860

ggcucugucg ggggagcucu caauagccug ggcaagggga uccaccagau uuucggcgcc     1920

gccuucaaau cccuguuugg aggcaugucu ugguucagcc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcuccauua gccugaugug ucuugcucuu     2040

gggggagugc ucaucuuucu gucuaccgcg guuuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8826
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 3
       opt

<400>  8826
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcugag cggaaagggc      900

ucccugguca caugcgccaa auucgccugc agcaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccg     1080

aauagcccca gagcggaagc cacccuugga ggcuuuggcu cccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu cucugaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ucacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggagu ucaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaagccag gaaggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucucuu auucccucug uaccgcugcc     1500

uuuacauuca ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagagag caccgaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uuuggcgacu ccuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcagcggau ccaccaucgg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuu     1860

ggcucuguug ggggagcucu caauagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuucaaau cccuguuugg aggcaugucu ugguucagcc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa cgcuaagaau ggcuccauca gccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuucu gucuaccgcc guguccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8827
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 3 
       opt

<400>  8827
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguc cggaaagggc      900

agccugguga ccugcgcuaa auucacaugc ucuaagaaga ugaccgggaa auccauccag      960

cccgagaauc ucgaauaucg cauuaugcug agcguucacg gaucucaaca uagcggcaug     1020

aucggguacg agacggacga agauagagcc aagguggagg ucacaccuaa cuccccgcgg     1080

gccgaggcua cccuuggagg cuuuggcucu cuggggcucg acugugaacc cagaaccgga     1140

cuggauuuca gcgaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag     1200

gagugguuuc acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau     1260

uggaauaaca aagaagcucu ggucgaguuc aaggaugccc acgccaaacg ccagacggug     1320

guugugcucg gaucccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggacg gcgccaaggg acggcuguuu agcggccacc ucaagugcag acugaaaaug     1440

gauaaacuuc gccugaaggg ggugucuuau ucccucugua cagcugccuu caccuuuaca     1500

aagguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gacgggccgu gcaaaauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacggcuca uuaccgccaa uccugugauc acggaaagca cagagaacuc uaagaugaug     1680

cuggaacuug auccucccuu cggcgacucc uacaucguga uuggggucgg cgauaagaaa     1740

aucacccacc acuggcauag aagcggaucc acaauuggca aggcuuuuga ggccaccgug     1800

cgcggggcua aacggauggc cguucuggga gacaccgcgu gggauuucgg cucugucggg     1860

ggaguguuca auagccucgg caaggggauc caccagauuu uuggcgccgc uuucaaaucc     1920

cuguuuggag gcaugucuug guucagccaa auccugaucg ggacacuucu cguguggcug     1980

ggacugaaca cgaagaacgg cuccauuagc cucaccuguc uggcccuugg gggaguuaug     2040

aucuuucugu cuacagcugu guccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8828
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F399S) mRNA Design 3 
       opt

<400>  8828
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcugag cggaaagggc      900

ucccugguga ccugcgccaa auucgccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccg     1080

aauagcccca gagcggaagc cacccuugga ggcuuuggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu cucugaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ucacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggagu ucaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaagccag gaaggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucucuu auucccucug uacagcugcc     1500

uuuaccuuca caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uuuggcgacu ccuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcagcggau ccacaaucgg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuu     1860

ggcucugucg ggggagcucu caauagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuucaaau cccuguuugg aggcaugucu ugguucagcc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcuccauca gccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuucu gucuaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8829
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       3 opt

<400>  8829
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugcagg acaccgggca ugaaacggau gagaaucggg cuaagguuga gaucacaccg     1080

aacucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucaa     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaga uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaucccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caacucucug ggcaagggga uccaccaaau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauccuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaagaac ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8830
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 3
       opt

<400>  8830
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguucagg acacggggca ugaaacagau gagaaucggg cuaaggugga gauuaccccg     1080

aacucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcaa     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaga uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaucccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccguug ggggagcucu caacucucug ggcaagggga uucaccaaau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa ugcuaagaac ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu gagcaccgcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8831
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 3 
       opt

<400>  8831
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguucagg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcaa     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaga uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaucccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caacucucug ggcaagggga uucaccaaau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauucuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaagaac ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8832
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8832
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacacuggg caaggccuuu     1800

uccaccaccc ucaaaggggc gcagcggcug gccgcucugg gagauacagc cugggacuuc     1860

ggcucuauug ggggaguguu caauagcauc ggcagagcug ugcaccaagu cuuugggggc     1920

gccuuccgca cgcuuuuugg aggcaugucc uggauuaccc aggggcucau gggagcccug     1980

cugcucugga ugggcgugaa cgcucgggau agaucuaucg cgcuggccuu ccuugcuaca     2040

gggggaguuc ugguguuucu cgccaccaau guccaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8833
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8833
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcacccuggg caaggccuuu     1800

uccaccacac ucaaaggggc gcagcggcug gccgcucugg gagauacggc cugggacuuc     1860

ggcucuaucg ggggaguguu caauagcauu ggcagagcug uccaccaagu guuugggggc     1920

gccuuccgca cccuuuuugg aggcaugucc uggaucacac aggggcucau gggagcccug     1980

cugcucugga ugggcguuaa cgcucgggau agaucuauug cgcuggccuu ccuugcuacc     2040

gggggagugc uggucuuucu cgccacaaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8834
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 3 opt

<400>  8834
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucggguacg agacggacga agauagagcc aagguggagg ucacaccuaa cagcccgcgg     1080

gccgaggcua cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga     1140

cuggauuuuu cugaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag     1200

gagugguucc acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau     1260

uggaauaaca aagaagcucu ggucgaguuu aaggaugccc acgccaaacg ccagacggug     1320

guugugcucg gaagccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggacg gcgccaaggg acggcuguuc uccggccacc ucaagugcag acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucucucugua cagcugccuu uaccuucaca     1500

aagguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gacgggccgu gcaaaauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacggcuca uuaccgccaa uccugugauc acggaaucca cagagaacag caagaugaug     1680

cuggaacuug auccucccuu uggcgacucu uacaucguga uuggggucgg cgauaagaaa     1740

aucacccacc acuggcauag auccggaagc acacugggca aggcuuucuc caccacccuc     1800

aaaggggccc agcgccuggc ugcccuggga gacacagcgu gggauuucgg cucuauuggg     1860

ggaguguuua auagcaucgg ccgggccguu caccaagugu ucgggggcgc uuuuagaacg     1920

cuuuucggag gcauguccug gauuacccag gggcucaugg gagcccugcu gcucuggaug     1980

ggcgucaacg cucgcgaccg gucuaucgcc cuggccuuuc uugcuacagg gggagugcug     2040

guuuuccucg cgaccaacgu gcaugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8835
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  8835
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacacuggg caaggccuuu     1800

uccaccaccc ucaaaggggc gcagcggcug gccgcucugg gagauacagc cugggacuuc     1860

ggcucuaucg ggggaguguu caauagcauu ggcagagcug uccaccaagu guuugggggc     1920

gccuuccgca cgcuuuuugg aggcaugucc uggaucaccc aggggcucau gggagcccug     1980

cugcucugga ugggcguuaa cgcucgggau agaucuauug cgcuggccuu ccuugcuaca     2040

gggggagugc uggucuuucu cgccaccaau gugcaugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8836
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  8836
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8837
<211>  2362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8837
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagcggcg cggcgcugac       60

accagcgugg ggaucgucgg acugcuccug acaacggcca uggcggcuga aguuaccaga      120

cggggcuccg ccuacuauau guaccuugau cgcaaugacg ccggggaggc uauuucuuuc      180

cccacaaccc ugggaaugaa caaaugcuau auccagauua uggaucucgg ccacaugugu      240

gacgccacaa ugagcuacga augcccuaug cuggaugagg ggguggaacc ggacgaugug      300

gacuguuggu gcaauaccac auccaccugg gucguguaug gaacguguca ucacaagaaa      360

ggcgaggcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      420

caaacccgcu cucagacaug gcucgagagc agagaauaca ccaagcaccu gauccgggug      480

gagaacugga ucuuucgcaa uccuggcuuc gcgcuggccg cugccgcuau ugccuggcuc      540

cuggggucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcgccc      600

gccuacucca uuagaugcau cggaguuagc aaccgggauu uuguggaagg caugucuggg      660

ggaacauggg ucgacauugu gcuggagcau ggcgggugug ugaccgucau ggcucaggau      720

aagccuacag uugacaucga gcuugugacc accacagugu ccaauauggc cgaaguccgc      780

agcuacugcu augaggcuuc uaucuccgau auggccagcg acuccagaug ucccacgcaa      840

ggagaagccu accuggacaa acagucugau acccaguaug ugugcaagcg gacacucguu      900

gacagaggcu ggggcaacgg guguggacug uucggcaaag ggagccuggu gaccugcgcu      960

aaguuugcgu gcuccaagaa aaugacagga aagagcauuc agccugagaa ucucgaauac     1020

cgcaucaugc ugucugucca cggcucccaa cacagcggga ugauugugaa cgauaccgga     1080

caugagacgg acgagaaucg ggccaaaguu gaaaucacac cgaacucucc cagagcugag     1140

gccacccuug gcggcuucgg gagccuggga cucgauugug aaccucgcac cggccuggac     1200

uuuuccgauc uuuauuaccu gacaaugaau aacaagcacu ggcucgugca uaaagagugg     1260

uuccacgaca uuccccugcc uuggcacgcc ggggcugaua ccggaacacc ccauuggaau     1320

aacaaggaag cccuggucga guuuaaagac gcgcacgcua agcggcagac gguggucgug     1380

cucggcucuc aggagggggc cguucauacc gcccuggcug gcgcccuuga agcugagaug     1440

gauggagcca agggcagacu gagcuccggg caccucaaau gccgccugaa gauggacaaa     1500

cuucggcuga agggagugag cuauucucuc uguacagcgg ccuucaccuu uacaaaaauc     1560

ccggcugaaa cccugcaugg cacggucacc guggagguuc aguacgccgg gacagacgga     1620

ccuugcaagg ugcccgcuca aauggccguc gauaugcaga cccugacacc ugugggcaga     1680

cucaucaccg cgaaccccgu gauuacggaa uccacagaga auagcaagau gaugcuggag     1740

cuugaucccc cuuucgggga cucuuacauc guuauuggcg ucggagagaa aaagaucacc     1800

caccacuggc aucgcuccgg cagcacaauu gggaaagccu uugaagcuac cgugcgggga     1860

gccaagagaa uggcugugcu gggcgacacc gccugggauu ucggguccgu cggaggcgcc     1920

cucaacucuc uggggaaagg cauccaccag aucuucggag cugcguuuaa gagccuguuc     1980

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacac uccugaugug gcugggccuc     2040

aauacgaaga acgggagcau uucccugaug ugucuugccc ugggaggcgu gcucaucuuc     2100

cugagcaccg cuguuucugc cgacgugggg ugcuccgucg auuuuagcaa augaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2340

ucuuuucaga gccaccagaa uu                                              2362


<210>  8838
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8838
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agcggcgcgg cgcugacacc       60

agcgugggga uugucggacu gcuccugaca acggccaugg cggcugaagu uaccagacgg      120

ggcuccgccu acuauaugua ccuugaucgc aaugacgccg gggaggcuau cucuuucccc      180

acaacccugg gaaugaacaa augcuauauu cagaucaugg aucucggcca caugugugac      240

gccacaauga gcuacgaaug cccuaugcug gaugaggggg uggaaccgga cgauguggac      300

uguuggugca auaccacauc caccuggguc guguauggaa cgugucauca caagaaaggc      360

gaggcuagac ggucucgcag agccguuacc cuucccagcc auuccacacg gaagcugcaa      420

acccgcucuc agacauggcu cgagagcaga gaauacacca agcaccugau ucggguggag      480

aacuggaucu uucgcaaucc uggcuucgcg cuggccgcug ccgcuaucgc cuggcuccug      540

ggguccagca cgucucagaa agucauuuau cuugugauga uccugcucau ugcgcccgcc      600

uacuccauca gaugcauugg aguuagcaac cgggauuuug uggaaggcau gucuggggga      660

acaugggucg acaucgugcu ggagcauggc ggguguguga ccgucauggc ucaggauaag      720

ccuacaguug acauugagcu ugugaccacc acagugucca auauggccga aguccgcagc      780

uacugcuaug aggcuucuau cuccgauaug gccagcgacu ccagaugucc cacgcaagga      840

gaagccuacc uggacaaaca gucugauacc caguaugugu gcaagcggac acucguugac      900

agaggcuggg gcaacgggug uggacuguuc ggcaaaggga gccuggugac cugcgcuaag      960

uuugcgugcu ccaagaaaau gacaggaaag agcauccagc cugagaaucu cgaauaccgc     1020

auuaugcugu cuguccacgg cucccaacac agcgggauga ucgugaacga uaccggacau     1080

gagacggacg agaaucgggc caaaguugaa auuacaccga acucucccag agcugaggcc     1140

acccuuggcg gcuucgggag ccugggacuc gauugugaac cucgcaccgg ccuggacuuu     1200

uccgaucuuu auuaccugac aaugaauaac aagcacuggc ucgugcauaa agagugguuc     1260

cacgacaucc cccugccuug gcacgccggg gcugauaccg gaacacccca uuggaauaac     1320

aaggaagccc uggucgaguu uaaagacgcg cacgcuaagc ggcagacggu ggucgugcuc     1380

ggcucucagg agggggccgu ucauaccgcc cuggcuggcg cccuugaagc ugagauggau     1440

ggagccaagg gcagacugag cuccgggcac cucaaaugcc gccugaagau ggacaaacuu     1500

cggcugaagg gagugagcua uucucucugu acagcggccu ucaccuuuac aaaaauuccg     1560

gcugaaaccc ugcauggcac ggucaccgug gagguucagu acgccgggac agacggaccu     1620

ugcaaggugc ccgcucaaau ggccgucgau augcagaccc ugacaccugu gggcagacuc     1680

aucaccgcga accccgugau cacggaaucc acagagaaua gcaagaugau gcuggagcuu     1740

gaucccccuu ucggggacuc uuacauuguu aucggcgucg gagagaaaaa gauuacccac     1800

cacuggcauc gcuccggcag cacaaucggg aaagccuuug aagcuaccgu gcggggagcc     1860

aagagaaugg cugugcuggg cgacaccgcc ugggauuucg gguccgucgg aggcgcccuc     1920

aacucucugg ggaaaggcau ucaccagauc uucggagcug cguuuaagag ccuguucggc     1980

gggauguccu gguuuucuca aauccuuauu ggaacacucc ugauguggcu gggccucaau     2040

acgaagaacg ggagcaucuc ccugaugugu cuugcccugg gaggcgugcu cauuuuccug     2100

agcaccgcug uuucugccga cguggggugc uccgucgauu uuagcaaaug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  8839
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8839
gggagaaagc uuaccaugaa cgccagaaag gagaaaaagc ggcgcggcgc ugacaccagc       60

guggggaucg ucggacugcu ccugacaacg gccauggcgg cugaaguuac cagacggggc      120

uccgccuacu auauguaccu ugaucgcaau gacgccgggg aggcuauuuc uuuccccaca      180

acccugggaa ugaacaaaug cuauauccag auuauggauc ucggccacau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggggugg aaccggacga uguggacugu      300

uggugcaaua ccacauccac cugggucgug uauggaacgu gucaucacaa gaaaggcgag      360

gcuagacggu cucgcagagc cguuacccuu cccagccauu ccacacggaa gcugcaaacc      420

cgcucucaga cauggcucga gagcagagaa uacaccaagc accugauccg gguggagaac      480

uggauuuuuc gcaauccugg cuucgcgcug gccgcugccg cuaucgccug gcuccugggg      540

uccagcacgu cucagaaagu caucuaucuu gugaugauuc ugcucaucgc gcccgccuac      600

uccauuagau gcaucggagu uagcaaccgg gauuuugugg aaggcauguc ugggggaaca      660

ugggucgaca uugugcugga gcauggcggg ugugugaccg ucauggcuca ggauaagccu      720

acaguugaca ucgagcuugu gaccaccaca guguccaaua uggccgaagu ccgcagcuac      780

ugcuaugagg cuucuauuuc cgauauggcc agcgacucca gaugucccac gcaaggagaa      840

gccuaccugg acaaacaguc ugauacccag uaugugugca agcggacacu cguugacaga      900

ggcuggggca acgggugugg acuguucggc aaagggagcc uggugaccug cgcuaaguuu      960

gcgugcucca agaaaaugac aggaaagagc auccagccug agaaucucga auaccgcauc     1020

augcugucug uccacggcuc ccaacacagc gggaugauug ugaacgauac cggacaugag     1080

acggacgaga aucgggccaa aguugaaauc acaccgaacu cucccagagc ugaggccacc     1140

cuuggcggcu ucgggagccu gggacucgau ugugaaccuc gcaccggccu ggacuuuucc     1200

gaucuuuauu accugacaau gaauaacaag cacuggcucg ugcauaaaga gugguuccac     1260

gacauucccc ugccuuggca cgccggggcu gauaccggaa caccccauug gaauaacaag     1320

gaagcccugg ucgaguuuaa agacgcgcac gcuaagcggc agacgguggu cgugcucggc     1380

ucucaggagg gggccguuca uaccgcccug gcuggcgccc uugaagcuga gauggaugga     1440

gccaagggca gacugagcuc cgggcaccuc aaaugccgcc ugaagaugga caaacuucgg     1500

cugaagggag ugagcuauuc ucucuguaca gcggccuuca ccuuuacaaa aaucccggcu     1560

gaaacccugc auggcacggu caccguggag guucaguacg ccgggacaga cggaccuugc     1620

aaggugcccg cucaaauggc cgucgauaug cagacccuga caccuguggg cagacucauu     1680

accgcgaacc ccgugaucac ggaauccaca gagaauagca agaugaugcu ggagcuugau     1740

cccccuuucg gggacucuua caucguuauu ggcgucggag agaaaaagau cacccaccac     1800

uggcaucgcu ccggcagcac aauugggaaa gccuuugaag cuaccgugcg gggagccaag     1860

agaauggcug ugcugggcga caccgccugg gauuucgggu ccgucggagg cgcccucaac     1920

ucucugggga aaggcaucca ccagauuuuc ggagcugcgu uuaagagccu guucggcggg     1980

auguccuggu uuucucaaau ccuuaucgga acacuccuga uguggcuggg ccucaauacg     2040

aagaacggga gcauuucccu gaugugucuu gcccugggag gcgugcucau cuuccugagc     2100

accgcuguuu cugccgacgu ggggugcucc gucgauuuua gcaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8840
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8840
gggagaaagc uuaccauggc cagaaaggag aaaaagcggc gcggcgcuga caccagcgug       60

gggaucgucg gacugcuccu gacaacggcc auggcggcug aaguuaccag acggggcucc      120

gccuacuaua uguaccuuga ucgcaacgac gccggggagg cuauuucuuu ccccacaacc      180

cugggaauga auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca      240

augagcuacg aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg      300

ugcaacacca cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu      360

agacggucuc gcagagccgu uacccuuccc agccauucca cacggaagcu gcaaacccgc      420

ucucagacau ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaauugg      480

auuuuucgca acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc      540

agcacgucuc agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc      600

auuagaugca ucggaguuag caaucgggau uuuguggaag gcaugucugg gggaacaugg      660

gucgacauug ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca      720

guugacaucg agcuugugac caccacagug uccaacaugg ccgaaguccg cagcuacugc      780

uaugaggcuu cuauuuccga uauggccagc gacuccagau gucccacgca aggagaagcc      840

uaccuggaca aacagucuga uacccaguau gugugcaagc ggacacucgu ugacagaggc      900

uggggcaaug gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg      960

ugcuccaaga aaaugacagg aaagagcauc cagccugaga accucgaaua ccgcaucaug     1020

cugucugucc acggcuccca acacagcggg augauuguga augauaccgg acaugagacg     1080

gacgagaacc gggccaaagu ugaaaucaca cccaauucuc ccagagcuga ggccacccuu     1140

ggcggcuucg ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau     1200

cuuuauuacc ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac     1260

auuccccugc cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa     1320

gcccuggucg aguuuaaaga cgcgcacgcu aagcggcaga cgguggucgu gcucggcucu     1380

caggaggggg ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc     1440

aagggcagac ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa     1560

acccugcaug gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag     1620

gugcccgcuc aaauggccgu cgauaugcag acccugacac cugugggcag acucauuacc     1680

gcgaaccccg ugaucacgga auccacagag aacagcaaga ugaugcugga gcuugacccg     1740

ccuuucgggg auucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaagcc uuugaagcua ccgugcgggg agccaagaga     1860

auggcugugc ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaauucu     1920

cuggggaaag gcauccacca gauuuucgga gcugcguuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag     2040

aaugggagca uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc     2100

gcuguuucug ccgacguggg gugcuccguc gauuuuagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8841
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8841
gggagaaagc uuaccaugag aaaggagaaa aagcggcgcg gcgccgacac cagcgugggg       60

aucgucggac ugcuccugac aacggcuaug gccgcggaag uuaccagacg gggcuccgcu      120

uacuauaugu accuugaucg caacgacgcc ggggaggcca uuucuuuccc cacaacccug      180

ggaaugaaua aaugcuauau ccagauuaug gaucucggcc acauguguga cgcuacaaug      240

agcuacgaau gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc      300

aacaccacau ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggccaga      360

cggucucgca gagcuguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu      420

cagacauggc ucgagagcag agaauacacc aagcaccuga uccgggugga gaauuggauu      480

uuucgcaacc cuggcuucgc ccuggcggcc gcugccaucg cuuggcuccu gggguccagc      540

acgucucaga aagucaucua ucuugugaug auucugcuca ucgcccccgc guacuccauu      600

agaugcaucg gaguuagcaa ucgggauuuu guggaaggca ugucuggggg aacauggguc      660

gacauugugc uggagcaugg cgggugugug accgucaugg cccaggauaa gccuacaguu      720

gacaucgagc uugugaccac cacagugucc aacauggcug aaguccgcag cuacugcuau      780

gaggccucua uuuccgauau ggcuagcgac uccagauguc ccacgcaagg agaagccuac      840

cuggacaaac agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg      900

ggcaaugggu guggacuguu cggcaaaggg agccugguga ccugcgccaa guuugcuugc      960

uccaagaaaa ugacaggaaa gagcauccag ccugagaacc ucgaauaccg caucaugcug     1020

ucuguccacg gcucccaaca cagcgggaug auugugaaug auaccggaca ugagacggac     1080

gagaaccggg cgaaaguuga aaucacaccc aauucuccca gagccgaggc uacccuuggc     1140

ggcuucggga gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu     1200

uauuaccuga caaugaacaa uaagcacugg cucgugcaua aagagugguu ccacgacauu     1260

ccccugccuu ggcacgccgg ggccgauacc ggaacacccc auuggaacaa uaaggaagcu     1320

cuggucgagu uuaaagacgc ccacgcgaag cggcagacgg uggucgugcu cggcucucag     1380

gagggggcug uucauaccgc ccuggccggc gcucuugaag ccgagaugga uggagcuaag     1440

ggcagacuga gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag     1500

ggagugagcu auucucucug uacagccgcg uucaccuuua caaaaauccc ggccgaaacc     1560

cugcauggca cggucaccgu ggagguucag uacgcuggga cagacggacc uugcaaggug     1620

cccgcccaaa uggcugucga uaugcagacc cugacaccug ugggcagacu cauuaccgcc     1680

aaccccguga ucacggaauc cacagagaac agcaagauga ugcuggagcu ugacccgccu     1740

uucggggauu cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau     1800

cgcuccggca gcacaauugg gaaagcguuu gaagccaccg ugcggggagc uaagagaaug     1860

gccgugcugg gcgacaccgc uugggauuuc ggguccgucg gaggcgcccu caauucucug     1920

gggaaaggca uccaccagau uuucggagcc gcuuuuaaga gccuguucgg cgggaugucc     1980

ugguuuucuc aaauccuuau cggaacacuc cugauguggc ugggccucaa cacgaagaau     2040

gggagcauuu cccugaugug ucuugcgcug ggaggcgugc ucaucuuccu gagcaccgcc     2100

guuucugcug acguggggug cuccgucgau uuuagcaaau gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2220

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  8842
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8842
gggagaaagc uuaccaugaa ggagaaaaag agacggggcg ccgacaccag cguggggauc       60

gucggacugc uccugacaac ggcuauggcc gcggaaguua cccgcagagg cuccgcuuac      120

uauauguacc uugaucggaa cgacgccggg gaggccauuu cuuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcccgcaga      360

ucucggcgcg cuguuacccu ucccagccau uccacaagaa agcugcaaac ccggucucag      420

acauggcucg agagccgcga auacaccaag caccugauca gaguggagaa uuggauuuuu      480

cggaacccug gcuucgcccu ggcggccgcu gccaucgcuu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cuccauucgc      600

ugcaucggag uuagcaauag agauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggccc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaac auggcugaag uccggagcua cugcuaugag      780

gccucuauuu ccgauauggc uagcgacucc cgcuguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg gggcuggggc      900

aaugggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaccucg aauacagaau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaaugaua ccggacauga gacggacgag     1080

aaccgcgcga aaguugaaau cacacccaau ucuccccggg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu agaaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagcgc cagacggugg ucgugcucgg cucucaggag     1380

ggggcuguuc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cggcugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccggcucau uaccgccaac     1680

cccgugauca cggaauccac agagaacagc aagaugaugc uggagcuuga cccgccuuuc     1740

ggggauucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcauaga     1800

uccggcagca caauugggaa agcguuugaa gccaccgugc gcggagcuaa gcggauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccgucggag gcgcccucaa uucucugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugcugacg uggggugcuc cgucgauuuu agcaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8843
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8843
gggagaaagc uuaccaugga gaagaaaaga cggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc uauggccgcg gaaguuaccc gcagaggcuc cgcuuacuau      120

auguaccuug aucggaacga cgccggggag gccauuucuu uccccacaac ccugggaaug      180

aauaagugcu auauccagau uauggaucuc ggccacaugu gugacgcuac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaacacc      300

acauccaccu gggucgugua uggaacgugu caucacaaaa agggcgaggc ccgcagaucu      360

cggcgcgcug uuacccuucc cagccauucc acaagaaaac ugcaaacccg gucucagaca      420

uggcucgaga gccgcgaaua caccaagcac cugaucagag uggagaauug gauuuuucgg      480

aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc uccugggguc cagcacgucu      540

cagaagguca ucuaucuugu gaugauucug cucaucgccc ccgcguacuc cauucgcugc      600

aucggaguua gcaauagaga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcccagg auaaaccuac aguugacauc      720

gagcuuguga ccaccacagu guccaacaug gcugaagucc ggagcuacug cuaugaggcc      780

ucuauuuccg auauggcuag cgacucccgc ugucccacgc aaggagaagc cuaccuggac      840

aagcagucug auacccagua ugugugcaaa agaacacucg uugaccgggg cuggggcaau      900

ggguguggac uguucggcaa ggggagccug gugaccugcg ccaaauuugc uugcuccaag      960

aagaugacag gaaaaagcau ccagccugag aaccucgaau acagaaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aaugauaccg gacaugagac ggacgagaac     1080

cgcgcgaagg uugaaaucac acccaauucu ccccgggccg aggcuacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccuaga accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga acaauaaaca cuggcucgug cauaaggagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggccga uaccggaaca ccccauugga acaauaaaga agcucugguc     1320

gaguuuaagg acgcccacgc gaaacgccag acgguggucg ugcucggcuc ucaggagggg     1380

gcuguucaua ccgcccuggc cggcgcucuu gaagccgaga uggauggagc uaagggccgg     1440

cugagcuccg ggcaccucaa gugcagacug aaaauggaca aacuucgccu gaagggagug     1500

agcuauucuc ucuguacagc cgcguucacc uuuacaaaga ucccggccga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcu gggacagacg gaccuugcaa agugcccgcc     1620

caaauggcug ucgauaugca gacccugaca ccugugggcc ggcucauuac cgccaacccc     1680

gugaucacgg aauccacaga gaacagcaag augaugcugg agcuugaccc gccuuucggg     1740

gauucuuaca ucguuauugg cgucggagaa aagaaaauca cccaccacug gcauagaucc     1800

ggcagcacaa uugggaaggc guuugaggcc accgugcgcg gagcuaaacg gauggccgug     1860

cugggcgaca ccgcuuggga uuucgggucc gucggaggcg cccucaauuc ucuggggaag     1920

ggcauccacc agauuuucgg agccgcuuuu aaaagccugu ucggcgggau guccugguuu     1980

ucucaaaucc uuaucggaac acuccugaug uggcugggcc ucaacacgaa gaaugggagc     2040

auuucccuga ugugucuugc gcugggaggc gugcucaucu uccugagcac cgccguuucu     2100

gcugacgugg ggugcuccgu cgauuuuagc aagugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  8844
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8844
gggagaaagc uuaccaugaa gaaaagacgg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuga caacggcuau ggccgcggag guuacccgca gaggcuccgc uuacuauaug      120

uaccuugauc ggaacgacgc cggggaagcc auuucuuucc ccacaacccu gggaaugaau      180

aagugcuaua uccagauuau ggaucucggc cacaugugug acgcuacaau gagcuacgag      240

ugcccuaugc uggaugaagg gguggagccg gacgaugugg acuguuggug caacaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaaaaagg gcgaagcccg cagaucucgg      360

cgcgcuguua cccuucccag ccauuccaca agaaaacugc aaacccgguc ucagacaugg      420

cucgagagcc gcgaguacac caagcaccug aucagagugg aaaauuggau uuuucggaac      480

ccuggcuucg cccuggcggc cgcugccauc gcuuggcucc ugggguccag cacgucucag      540

aaggucaucu aucuugugau gauucugcuc aucgcccccg cguacuccau ucgcugcauc      600

ggaguuagca auagagauuu uguggagggc augucugggg gaacaugggu cgacauugug      660

cuggaacaug gcgggugugu gaccgucaug gcccaggaua aaccuacagu ugacaucgag      720

cuugugacca ccacaguguc caacauggcu gagguccgga gcuacugcua ugaagccucu      780

auuuccgaua uggcuagcga cucccgcugu cccacgcaag gagaggccua ccuggacaag      840

cagucugaua cccaguaugu gugcaaaaga acacucguug accggggcug gggcaauggg      900

uguggacugu ucggcaaggg gagccuggug accugcgcca aauuugcuug cuccaagaag      960

augacaggaa aaagcaucca gccugaaaac cucgaguaca gaaucaugcu gucuguccac     1020

ggcucccaac acagcgggau gauugugaau gauaccggac augaaacgga cgagaaccgc     1080

gcgaagguug agaucacacc caauucuccc cgggccgaag cuacccuugg cggcuucggg     1140

agccugggac ucgauuguga gccuagaacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaca auaaacacug gcucgugcau aaggaauggu uccacgacau uccccugccu     1260

uggcacgccg gggccgauac cggaacaccc cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggacg cccacgcgaa acgccagacg guggucgugc ucggcucuca ggagggggcu     1380

guucauaccg cccuggccgg cgcucuugag gccgaaaugg auggagcuaa gggccggcug     1440

agcuccgggc accucaagug cagacugaaa auggacaaac uucgccugaa gggagugagc     1500

uauucucucu guacagccgc guucaccuuu acaaagaucc cggccgagac ccugcauggc     1560

acggucaccg uggaaguuca guacgcuggg acagacggac cuugcaaagu gcccgcccaa     1620

auggcugucg auaugcagac ccugacaccu gugggccggc ucauuaccgc caaccccgug     1680

aucacggagu ccacagaaaa cagcaagaug augcuggagc uugacccgcc uuucggggau     1740

ucuuacaucg uuauuggcgu cggagagaag aaaaucaccc accacuggca uagauccggc     1800

agcacaauug ggaaggcguu ugaagccacc gugcgcggag cuaaacggau ggccgugcug     1860

ggcgacaccg cuugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaagggc     1920

auccaccaga uuuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu     2040

ucccugaugu gucuugcgcu gggaggcgug cucaucuucc ugagcaccgc cguuucugcu     2100

gacguggggu gcuccgucga uuuuagcaag ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  8845
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8845
gggagaaagc uuaccaugaa gagacggggc gccgacacca gcguggggau cgucggacug       60

cuccugacaa cggcuauggc cgcggagguu acccgcagag gcuccgcuua cuauauguac      120

cuugaucgga acgacgccgg ggaagccauu ucuuucccca caacccuggg aaugaauaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg cuacaaugag cuacgagugc      240

ccuaugcugg augaaggggu ggagccggac gauguggacu guuggugcaa caccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aagcccgcag aucucggcgc      360

gcuguuaccc uucccagcca uuccacaaga aagcugcaaa cccggucuca gacauggcuc      420

gagagccgcg aguacaccaa acaccugauc agaguggaaa auuggauuuu ucggaacccu      480

ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaag      540

gucaucuauc uugugaugau ucugcucauc gcccccgcgu acuccauucg cugcaucgga      600

guuagcaaua gagauuuugu ggagggcaug ucugggggaa caugggucga cauugugcug      660

gaacauggcg ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa cauggcugag guccggagcu acugcuauga agccucuauu      780

uccgauaugg cuagcgacuc ccgcuguccc acgcaaggag aggccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagagaaca cucguugacc ggggcugggg caaugggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caaaaagaug      960

acaggaaaga gcauccagcc ugaaaaccuc gaguacagaa ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaaugau accggacaug aaacggacga gaaccgcgcg     1080

aaaguugaga ucacacccaa uucuccccgg gccgaagcua cccuuggcgg cuucgggagc     1140

cugggacucg auugugagcc uagaaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaacaaua agcacuggcu cgugcauaaa gaaugguucc acgacauucc ccugccuugg     1260

cacgccgggg ccgauaccgg aacaccccau uggaacaaua aggaggcucu ggucgaauuu     1320

aaagacgccc acgcgaagcg ccagacggug gucgugcucg gcucucagga gggggcuguu     1380

cauaccgccc uggccggcgc ucuugaggcc gaaauggaug gagcuaaagg ccggcugagc     1440

uccgggcacc ucaagugcag acugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucucucugua cagccgcguu caccuuuaca aaaaucccgg ccgagacccu gcauggcacg     1560

gucaccgugg aaguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug     1620

gcugucgaua ugcagacccu gacaccugug ggccggcuca uuaccgccaa ccccgugauc     1680

acggagucca cagaaaacag caaaaugaug cuggagcuug acccgccuuu cggggauucu     1740

uacaucguua uuggcgucgg agagaagaag aucacccacc acuggcauag auccggcagc     1800

acaauuggga aagcguuuga agccaccgug cgcggagcua agcggauggc cgugcugggc     1860

gacaccgcuu gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaca cgaaaaaugg gagcauuucc     2040

cugauguguc uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac     2100

guggggugcu ccgucgauuu uagcaaguga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8846
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8846
gggagaaagc uuaccaugag acggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg cuauggccgc ggagguuacc cgcagaggcu ccgcuuacua uauguaccuu      120

gaucggaacg acgccgggga agccauuucu uuccccacaa cccugggaau gaauaagugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcua caaugagcua cgagugcccu      240

augcuggaug aaggggugga gccggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaaa aagggcgaag cccgcagauc ucggcgcgcu      360

guuacccuuc ccagccauuc cacaagaaaa cugcaaaccc ggucucagac auggcucgag      420

agccgcgagu acaccaagca ccugaucaga guggaaaauu ggauuuuucg gaacccuggc      480

uucgcccugg cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu      600

agcaauagag auuuugugga gggcaugucu gggggaacau gggucgacau ugugcuggaa      660

cauggcgggu gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggcugagguc cggagcuacu gcuaugaagc cucuauuucc      780

gauauggcua gcgacucccg cugucccacg caaggagagg ccuaccugga caagcagucu      840

gauacccagu augugugcaa aagaacacuc guugaccggg gcuggggcaa ugggugugga      900

cuguucggca aggggagccu ggugaccugc gccaaauuug cuugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga aaaccucgag uacagaauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaaa cggacgagaa ccgcgcgaag     1080

guugagauca cacccaauuc uccccgggcc gaagcuaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugagccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccccu gccuuggcac     1260

gccggggccg auaccggaac accccauugg aacaauaaag aggcucuggu cgaauuuaag     1320

gacgcccacg cgaaacgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau     1380

accgcccugg ccggcgcucu ugaggccgaa auggauggag cuaagggccg gcugagcucc     1440

gggcaccuca aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu     1500

cucuguacag ccgcguucac cuuuacaaag aucccggccg agacccugca uggcacgguc     1560

accguggaag uucaguacgc ugggacagac ggaccuugca aagugcccgc ccaaauggcu     1620

gucgauaugc agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca     1800

auugggaagg cguuugaagc caccgugcgc ggagcuaaac ggauggccgu gcugggcgac     1860

accgcuuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa gggcauccac     1920

cagauuuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacgug     2100

gggugcuccg ucgauuuuag caaaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8847
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8847
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga gguuacccgg cgcggcuccg cuuacuauau guaccuugau      120

agaaacgacg ccggggaagc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga gugcccuaug      240

cuggaugaag ggguggagcc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaagccc ggcgcucuag acgggcuguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgagagc      420

cgggaguaca ccaagcaccu gauccgcgug gaaaauugga uuuuuagaaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaggg caugucuggg ggaacauggg ucgacauugu gcuggaacau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaggucaga agcuacugcu augaagccuc uauuuccgau      780

auggcuagcg acucccggug ucccacgcaa ggagaggccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugaaaa ccucgaguac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugaaacgg acgagaacag agcgaagguu     1080

gagaucacac ccaauucucc ccgcgccgaa gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug agccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggaaugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaagagg cucuggucga auuuaaggac     1320

gcccacgcga aaagacagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga ggccgaaaug gauggagcua agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaagauc ccggccgaga cccugcaugg cacggucacc     1560

guggaaguuc aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg ccaaccccgu gaucacggag     1680

uccacagaaa acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggcgu uugaagccac cgugagagga gcuaaacgca uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacgugggg     2100

ugcuccgucg auuuuagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8848
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8848
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaggu uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

aacgacgccg gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug      240

gaugaagggg uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caaaaagggc gaagccagac ggucucgcag agcuguuacc      360

cuucccagcc auuccacacg gaaacugcaa acccgcucuc agacauggcu cgagagcaga      420

gaguacacca agcaccugau ccggguggaa aauuggauuu uucgcaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauua gaugcaucgg aguuagcaau      600

cgggauuuug uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga gguccgcagc uacugcuaug aagccucuau uuccgauaug      780

gcuagcgacu ccagaugucc cacgcaagga gaggccuacc uggacaagca gucugauacc      840

caguaugugu gcaaacggac acucguugac cgcggcuggg gcaaugggug uggacuguuc      900

ggcaagggga gccuggugac cugcgccaaa uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gaaacggacg agaaccgggc gaagguugag     1080

aucacaccca auucucccag agccgaagcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugagc cucgcaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc     1320

cacgcgaaac ggcagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaggc cgaaauggau ggagcuaagg gcagacugag cuccgggcac     1440

cucaaaugcc gccugaagau ggacaaacuu cggcugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug     1560

gaaguucagu acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggcagacuc auuaccgcca accccgugau cacggagucc     1680

acagaaaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcauc gcuccggcag cacaauuggg     1800

aaggcguuug aagccaccgu gcggggagcu aaaagaaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu     1920

uucggagccg cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga cguggggugc     2100

uccgucgauu uuagcaaaug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8849
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8849
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccugacaacg       60

gcuauggccg cggagguuac cagacgggga uccgcuuacu auauguaccu ugaucgcaac      120

gacgccggcg aagccauuuc uuuccccaca acccugggga ugaauaagug cuauauccag      180

auuauggauc ucggacacau gugugacgcu acaaugagcu acgagugccc uaugcuggau      240

gaaggcgugg agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uaugggacgu gucaucacaa aaagggagaa gccagacggu cucgcagagc uguuacccuu      360

cccagccauu ccacacggaa acugcaaacc cgcucucaga cauggcucga gagcagagag      420

uacaccaagc accugauccg gguggaaaau uggauuuuuc gcaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggc uccagcacgu cucagaaagu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauuagau gcaucggggu uagcaaucgg      600

gauuuugugg agggaauguc uggcgggaca ugggucgaca uugugcugga acauggaggc      660

ugugugaccg ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaggu ccgcagcuac ugcuaugaag ccucuauuuc cgauauggcu      780

agcgacucca gaugucccac gcaaggggag gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aacggacacu cguugaccgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucca agaaaaugac aggaaagagc      960

auccagccug aaaaccucga guacagaauc augcugucug uccacggguc ccaacacagc     1020

ggcaugauug ugaaugauac cgggcaugaa acggacgaga accgggcgaa gguugagauc     1080

acacccaauu cucccagagc cgaagcuacc cuuggaggcu ucggcagccu ggggcucgau     1140

ugugagccuc gcaccggacu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga augguuccac gacauucccc ugccuuggca cgccggcgcc     1260

gauaccggga caccccauug gaacaauaaa gaggcucugg ucgaauuuaa ggacgcccac     1320

gcgaaacggc agacgguggu cgugcucgga ucucaggagg gcgcuguuca uaccgcccug     1380

gccggggcuc uugaggccga aauggauggc gcuaagggaa gacugagcuc cggccaccuc     1440

aaaugccgcc ugaagaugga caagcugcgg cugaaagggg ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gagacccugc auggaacggu caccguggaa     1560

guucaguacg cuggcacaga cgggccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg aagacucauu accgccaacc ccgugaucac ggaguccaca     1680

gaaaacagca agaugaugcu ggagcuugac ccgccuuucg gcgauucuua caucguuauu     1740

ggggucggcg agaaaaagau cacccaccac uggcaucgcu ccggaagcac aauuggcaag     1800

gcguuugaag ccaccgugag aggggcuaaa cggauggccg ugcugggaga caccgcuugg     1860

gauuucggcu ccgucggggg agcccucaau ucucugggca aggggaucca ccagauuuuc     1920

ggcgccgcuu uuaaaagccu guucggaggc auguccuggu uuucucaaau ccuuaucggg     1980

acacuccuga uguggcuggg acucaacacg aagaauggca gcauuucccu gaugugucuu     2040

gcgcuugggg gagugcucau cuuccugagc accgccguuu cugcugacgu gggcugcucc     2100

gucgauuuua gcaaaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8850
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8850
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu gacaacggcc       60

auggcugccg agguuaccag acggggaucc gcguacuaua uguaccuuga ucgcaacgac      120

gcuggcgaag ccauuucuuu ccccacaacc cuggggauga auaagugcua uauccagauu      180

auggaucucg gacacaugug ugacgccaca augagcuacg agugcccuau gcuggaugaa      240

ggcguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

gggacguguc aucacaaaaa gggagaagcu agacggucuc gcagagccgu uacccuuccc      360

agccauucca cacggaaacu gcaaacccgc ucucagacau ggcucgagag cagagaguac      420

accaagcacc ugauccgggu ggaaaauugg auuuuucgca acccuggcuu cgcucuggcc      480

gcggccgcua ucgccuggcu ccugggcucc agcacgucuc agaaagucau cuaucuugug      540

augauucugc ucaucgcucc cgccuacucc auuagaugca ucgggguuag caaucgggau      600

uuuguggagg gaaugucugg cgggacaugg gucgacauug ugcuggaaca uggaggcugu      660

gugaccguca uggcgcagga uaagccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg ccgagguccg cagcuacugc uaugaagcua gcauuuccga uauggccagc      780

gacuccagau gucccacgca aggggaggcu uaccuggaca agcagucuga uacccaguau      840

gugugcaaac ggacacucgu ugaccgcgga uggggcaaug gcugugggcu guucggaaag      900

ggcagccugg ugaccugcgc caaauuugcc ugcuccaaga aaaugacagg gaagucuauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggauccca acacagcggc     1020

augauuguga augauaccgg gcaugaaacg gacgagaacc gggcuaaggu ugagaucaca     1080

cccaauucuc ccagagcgga agccacccuu ggaggcuucg gcagccuggg gcucgauugu     1140

gagccucgca ccggacugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc uggcgccgau     1260

accgggacac cccauuggaa caauaaagag gcccuggucg aauuuaagga cgcucacgcc     1320

aaacggcaga cgguggucgu gcucggaucu caggagggcg cgguucauac cgcucuggcc     1380

ggggcccuug aggcugaaau ggauggcgcc aagggaagac ugagcuccgg ccaccucaaa     1440

ugccgccuga agauggacaa gcugcggcug aaagggguga gcuauucucu cuguacagcu     1500

gccuucaccu uuacaaagau cccggcggag acccugcaug gaacggucac cguggaaguu     1560

caguacgccg gcacagacgg gccuugcaaa gugcccgcuc aaauggccgu cgauaugcag     1620

acccugacac cugugggaag acucauuacc gcuaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucggcg auucuuacau cguuauuggg     1740

gucggcgaga aaaagaucac ccaccacugg caucgcuccg gaagcacaau uggcaaggcc     1800

uuugaagcga ccgugagagg ggccaaacgg auggcugugc ugggagacac cgccugggau     1860

uucggcuccg ucgggggagc ucucaauucu cugggcaagg ggauccacca gauuuucggc     1920

gccgccuuua aaagccuguu cggaggcaug uccugguuuu cucaaauccu uaucgggaca     1980

cuccugaugu ggcugggacu caacacgaag aauggcagca uuucccugau gugucuugcu     2040

cuugggggag ugcucaucuu ccugagcacc gcgguuucug ccgacguggg cugcuccguc     2100

gauuuuagca aaugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8851
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8851
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccg augugggcug cuccgucgac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8852
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8852
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgcgg uuaccagacg gggauccgcu uacuauaugu accuugaccg caacgaugcc      120

ggcgaggcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugcuacaaug agcuacgaau gcccuaugcu ggacgagggc      240

guggaaccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaggccaga cggucucgca gagcuguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgaaagcag agaguacacc      420

aagcaccuga uccgggugga gaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggaaggaa ugucuggcgg gacauggguc gauauugugc uggagcaugg aggcugugug      660

accgucaugg cccaggacaa gccuacaguu gauaucgaac uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaggccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaagccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauccag      960

ccugagaacc ucgaauacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugagacggau gaaaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaggc uacccuugga ggcuucggca gccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccccugccuu ggcacgccgg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaagcu cuggucgagu uuaaggaugc ccacgcgaaa     1320

cggcagacgg uggucgugcu cggaucucag gaaggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgagaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgaaacc cugcauggaa cggucaccgu ggagguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaggccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcug augugggcug cuccgucgac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8853
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8853
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcggc uguuacccgg cgcggcuccg ccuacuauau guaccuugau      120

agaaacgacg ccggggaggc uauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgccacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaggcuc ggcgcucuag acgggccguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgaaagc      420

cgggaguaca ccaagcaccu gauccgcgug gagaauugga uuuuuagaaa cccuggcuuc      480

gcgcuggccg cugccgcuau cgccuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgcgccc gccuacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcucaggau aagccuacag uugacaucga acuugugacc      720

accacagugu ccaacauggc cgaggucaga agcuacugcu augaggcuuc uauuuccgau      780

auggccagcg acucccggug ucccacgcaa ggagaagccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcu aaauuugcgu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgaaaacag agccaagguu     1080

gagaucacac ccaauucucc ccgcgcugag gccacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggcugaua ccggaacacc ccauuggaac aauaaagaag cccuggucga guuuaaggac     1320

gcgcacgcua aaagacagac gguggucgug cucggcucuc aggaaggggc cguucauacc     1380

gcccuggcug gcgcccuuga ggcugagaug gauggagcca agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagcgg ccuucaccuu uacaaagauc ccggcugaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgccgg gacagacgga ccuugcaaag ugcccgcuca aauggccguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg cgaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggaa cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggccu uugaggcuac cgugagagga gccaaacgca uggcugugcu gggcgacacc     1860

gccugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag cugcguuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugccc ugggaggcgu gcucaucuuc cugagcaccg cuguuucugc cgacgugggg     2100

ugcuccgucg auuuuagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8854
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8854
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gaggcguuac gcggcgcggg      120

agcgccuacu auauguaccu ggauagaaac gacgcuggag aggccauuuc cuuucccacc      180

acacuuggca ugaauaaaug cuauauccaa auuauggauc uggggcauau gugugacgcg      240

accaugucuu acgaaugccc uaugcucgau gagggagugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggcaccu gucaccauaa gaaaggcgag      360

gcucggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg guuugcccuu gcugccgcug ccaucgcgug gcugcucgga      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ccccgcuuac      600

ucuauucggu gcaucggcgu uuccaaucgc gauuucgugg aagggaugag cggaggcacg      660

ugggucgaca uugugcucga gcacggggga ugugugacag ucauggccca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggcugaggu cagauccuac      780

ugcuaugagg ccagcauuuc cgauauggcg ucugacagcc ggugucccac acagggcgaa      840

gccuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggga auggaugugg ccuguuuggg aaaggaagcc uugugacaug cgcuaaguuc      960

gccugcucua aaaagaugac cggcaagucc auccaaccug agaaccugga auaccggauc     1020

augcucagcg uccacggguc ucagcauagc ggaaugauug ugaaugauac aggccacgag     1080

accgacgaaa acagagcuaa aguugagauc acgcccaauu ccccgcgcgc cgaggccaca     1140

cugggcgggu uuggaucucu uggccuggau ugugaaccuc ggaccgggcu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcuggagcg gauacaggca caccccacug gaacaauaag     1320

gaagcccuug ucgaguucaa agacgcucau gccaagagac agacaguggu cgugcugggg     1380

ucccaggaag gcgccguuca cacggcucuc gccggagcgc uggaggcuga gauggauggc     1440

gccaaagggc gccuuagcuc uggacaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggcg uguccuauag ccucuguacc gccgcuuuua cauucaccaa aaucccggcc     1560

gaaacacugc acgggaccgu cacgguggag guucaauacg cuggaaccga cggcccuugc     1620

aaggugcccg cccagauggc ggucgauaug cagacacuua ccccuguggg gcgccugauu     1680

acagccaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gcgacagcua caucguuauu ggagucggcg agaagaagau cacacaccau     1800

uggcaccggu ccgggucuac cauuggaaag gcuuucgagg ccacagugag aggcgcuaaa     1860

cgcauggccg ugcuggggga caccgcgugg gauuucggaa gcgucggcgg ggcccuuaau     1920

ucccucggca aaggaaucca ucaaauuuuu ggcgcugccu ucaagucucu guuuggggga     1980

augagcuggu ucucccagau ccugaucggc acccuccuga uguggcuugg gcugaacacc     2040

aaaaauggaa gcauuucucu caugugucug gcucugggcg gggugcuuau cuuucucucc     2100

acggccguua gcgccgacgu gggcugcucu gucgauuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8855
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8855
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gagccguuac gcggcgcggc      120

agcgcuuacu auauguaccu ggauagaaac gacgccgggg aggcgauuuc cuuucccacc      180

acacuuggaa ugaauaaaug cuauauccaa auuauggauc ugggccauau gugugacgcu      240

accaugucuu acgaaugccc uaugcucgau gagggggugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggaaccu gucaccauaa gaaaggcgag      360

gcccggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg cuuugcucuu gccgcugccg cgaucgccug gcugcucggg      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ucccgccuac      600

ucuauucggu gcaucggagu uuccaaucgc gauuucgugg aaggcaugag cgggggaacg      660

ugggucgaca uugugcucga gcacggcggg ugugugacag ucauggcuca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggccgaggu cagauccuac      780

ugcuaugagg cgagcauuuc cgauauggcc ucugacagcc ggugucccac acagggagag      840

gcuuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggca augggugugg acuguuuggc aaagggagcc uugugacaug cgccaaguuc      960

gcuugcucua aaaagaugac cggaaagucc auccaaccug aaaaccugga auaccggauc     1020

augcucagcg uccacggcuc ucagcauagc gggaugauug ugaaugauac aggacacgag     1080

accgacgaaa acagagccaa aguugagauc acgcccaauu ccccgcgcgc cgaggcuaca     1140

cugggcggcu uugggucucu uggacuggau ugugaaccuc ggaccggccu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcgggggcc gauacaggaa caccccacug gaacaauaag     1320

gaagcucuug ucgaguucaa agacgcccau gccaagagac agacaguggu cgugcugggc     1380

ucccaggaag gggcuguuca cacggcccuc gcgggcgcuc uggaggccga gauggaugga     1440

gccaaaggcc gccuuagcuc ugggcaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggag uguccuauag ccucuguacc gcugccuuua cauucaccaa aaucccggcu     1560

gaaacacugc acggcaccgu cacgguggag guucaauacg ccgggaccga cggaccuugc     1620

aaggugcccg cgcagauggc cgucgauaug cagacacuua ccccuguggg ccgccugauu     1680

acagcuaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gggacagcua caucguuauu ggcgucggag agaagaagau cacacaccau     1800

uggcaccggu ccggcucuac cauugggaaa gccuucgagg cuacagugag aggagccaag     1860

cgcauggcgg ugcugggcga caccgccugg gauuucggga gcgucggagg cgcucuuaau     1920

ucccucggga aaggcaucca ucaaauuuuu ggagccgcuu ucaagucucu guuuggcggg     1980

augagcuggu ucucccagau ccugaucgga acccuccuga uguggcuugg ccugaacacc     2040

aaaaauggga gcauuucucu caugugucug gcccugggag gcgugcuuau cuuucucucc     2100

acggccguua gcgcugacgu ggggugcucu gucgauuucu ccaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  8856
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8856
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8857
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8857
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagcggcg cggcgcugac       60

accagcgugg ggaucgucgg acugcuccug acaacggcca uggcggcuga aguuaccaga      120

cggggcuccg ccuacuauau guaccuugau cgcaaugacg ccggggaggc uauuucuuuc      180

cccacaaccc ugggaaugaa caaaugcuau auccagauua uggaucucgg ccacaugugu      240

gacgccacaa ugagcuacga augcccuaug cuggaugagg ggguggaacc ggacgaugug      300

gacuguuggu gcaauaccac auccaccugg gucguguaug gaacguguca ucacaagaaa      360

ggcgaggcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      420

caaacccgcu cucagacaug gcucgagagc agagaauaca ccaagcaccu gauccgggug      480

gagaacugga ucuuucgcaa uccuggcuuc gcgcuggccg cugccgcuau ugccuggcuc      540

cuggggucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcgccc      600

gccuacucca uuagaugcau cggaguuagc aaccgggauu uuguggaagg caugucuggg      660

ggaacauggg ucgacauugu gcuggagcau ggcgggugug ugaccgucau ggcucaggau      720

aagccuacag uugacaucga gcuugugacc accacagugu ccaauauggc cgaaguccgc      780

agcuacugcu augaggcuuc uaucuccgau auggccagcg acuccagaug ucccacgcaa      840

ggagaagccu accuggacaa acagucugau acccaguaug ugugcaagcg gacacucguu      900

gacagaggcu ggggcaacgg guguggacug uucggcaaag ggagccuggu gaccugcgcu      960

aaguuugcgu gcuccaagaa aaugacagga aagagcauuc agccugagaa ucucgaauac     1020

cgcaucaugc ugucugucca cggcucccaa cacagcggga ugauugugaa cgauaccgga     1080

caugagacgg acgagaaucg ggccaaaguu gaaaucacac cgaacucucc cagagcugag     1140

gccacccuug gcggcuucgg gagccuggga cucgauugug aaccucgcac cggccuggac     1200

uuuuccgauc uuuauuaccu gacaaugaau aacaagcacu ggcucgugca uaaagagugg     1260

uuccacgaca uuccccugcc uuggcacgcc ggggcugaua ccggaacacc ccauuggaau     1320

aacaaggaag cccuggucga guuuaaagac gcgcacgcua agcggcagac gguggucgug     1380

cucggcucuc aggagggggc cguucauacc gcccuggcug gcgcccuuga agcugagaug     1440

gauggagcca agggcagacu gagcuccggg caccucaaau gccgccugaa gauggacaaa     1500

cuucggcuga agggagugag cuauucucuc uguacagcgg ccuucaccuu uacaaaaauc     1560

ccggcugaaa cccugcaugg cacggucacc guggagguuc aguacgccgg gacagacgga     1620

ccuugcaagg ugcccgcuca aauggccguc gauaugcaga cccugacacc ugugggcaga     1680

cucaucaccg cgaaccccgu gauuacggaa uccacagaga auagcaagau gaugcuggag     1740

cuugaucccc cuuucgggga cucuuacauc guuauuggcg ucggagagaa aaagaucacc     1800

caccacuggc aucgcuccgg cagcacaauu gggaaagccu uugaagcuac cgugcgggga     1860

gccaagagaa uggcugugcu gggcgacacc gccugggauu ucggguccgu cggaggcgcc     1920

cucaacucuc uggggaaagg cauccaccag aucuucggag cugcguuuaa gagccuguuc     1980

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacac uccugaugug gcugggccuc     2040

aauacgaaga acgggagcau uucccugaug ugucuugccc ugggaggcgu gcucaucuuc     2100

cugagcaccg cuguuucugc cgacgugggg ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  8858
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8858
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agcggcgcgg cgcugacacc       60

agcgugggga uugucggacu gcuccugaca acggccaugg cggcugaagu uaccagacgg      120

ggcuccgccu acuauaugua ccuugaucgc aaugacgccg gggaggcuau cucuuucccc      180

acaacccugg gaaugaacaa augcuauauu cagaucaugg aucucggcca caugugugac      240

gccacaauga gcuacgaaug cccuaugcug gaugaggggg uggaaccgga cgauguggac      300

uguuggugca auaccacauc caccuggguc guguauggaa cgugucauca caagaaaggc      360

gaggcuagac ggucucgcag agccguuacc cuucccagcc auuccacacg gaagcugcaa      420

acccgcucuc agacauggcu cgagagcaga gaauacacca agcaccugau ucggguggag      480

aacuggaucu uucgcaaucc uggcuucgcg cuggccgcug ccgcuaucgc cuggcuccug      540

ggguccagca cgucucagaa agucauuuau cuugugauga uccugcucau ugcgcccgcc      600

uacuccauca gaugcauugg aguuagcaac cgggauuuug uggaaggcau gucuggggga      660

acaugggucg acaucgugcu ggagcauggc ggguguguga ccgucauggc ucaggauaag      720

ccuacaguug acauugagcu ugugaccacc acagugucca auauggccga aguccgcagc      780

uacugcuaug aggcuucuau cuccgauaug gccagcgacu ccagaugucc cacgcaagga      840

gaagccuacc uggacaaaca gucugauacc caguaugugu gcaagcggac acucguugac      900

agaggcuggg gcaacgggug uggacuguuc ggcaaaggga gccuggugac cugcgcuaag      960

uuugcgugcu ccaagaaaau gacaggaaag agcauccagc cugagaaucu cgaauaccgc     1020

auuaugcugu cuguccacgg cucccaacac agcgggauga ucgugaacga uaccggacau     1080

gagacggacg agaaucgggc caaaguugaa auuacaccga acucucccag agcugaggcc     1140

acccuuggcg gcuucgggag ccugggacuc gauugugaac cucgcaccgg ccuggacuuu     1200

uccgaucuuu auuaccugac aaugaauaac aagcacuggc ucgugcauaa agagugguuc     1260

cacgacaucc cccugccuug gcacgccggg gcugauaccg gaacacccca uuggaauaac     1320

aaggaagccc uggucgaguu uaaagacgcg cacgcuaagc ggcagacggu ggucgugcuc     1380

ggcucucagg agggggccgu ucauaccgcc cuggcuggcg cccuugaagc ugagauggau     1440

ggagccaagg gcagacugag cuccgggcac cucaaaugcc gccugaagau ggacaaacuu     1500

cggcugaagg gagugagcua uucucucugu acagcggccu ucaccuuuac aaaaauuccg     1560

gcugaaaccc ugcauggcac ggucaccgug gagguucagu acgccgggac agacggaccu     1620

ugcaaggugc ccgcucaaau ggccgucgau augcagaccc ugacaccugu gggcagacuc     1680

aucaccgcga accccgugau cacggaaucc acagagaaua gcaagaugau gcuggagcuu     1740

gaucccccuu ucggggacuc uuacauuguu aucggcgucg gagagaaaaa gauuacccac     1800

cacuggcauc gcuccggcag cacaaucggg aaagccuuug aagcuaccgu gcggggagcc     1860

aagagaaugg cugugcuggg cgacaccgcc ugggauuucg gguccgucgg aggcgcccuc     1920

aacucucugg ggaaaggcau ucaccagauc uucggagcug cguuuaagag ccuguucggc     1980

gggauguccu gguuuucuca aauccuuauu ggaacacucc ugauguggcu gggccucaau     2040

acgaagaacg ggagcaucuc ccugaugugu cuugcccugg gaggcgugcu cauuuuccug     2100

agcaccgcug uuucugccga cguggggugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  8859
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8859
gggagaaagc uuaccaugaa cgccagaaag gagaaaaagc ggcgcggcgc ugacaccagc       60

guggggaucg ucggacugcu ccugacaacg gccauggcgg cugaaguuac cagacggggc      120

uccgccuacu auauguaccu ugaucgcaau gacgccgggg aggcuauuuc uuuccccaca      180

acccugggaa ugaacaaaug cuauauccag auuauggauc ucggccacau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggggugg aaccggacga uguggacugu      300

uggugcaaua ccacauccac cugggucgug uauggaacgu gucaucacaa gaaaggcgag      360

gcuagacggu cucgcagagc cguuacccuu cccagccauu ccacacggaa gcugcaaacc      420

cgcucucaga cauggcucga gagcagagaa uacaccaagc accugauccg gguggagaac      480

uggauuuuuc gcaauccugg cuucgcgcug gccgcugccg cuaucgccug gcuccugggg      540

uccagcacgu cucagaaagu caucuaucuu gugaugauuc ugcucaucgc gcccgccuac      600

uccauuagau gcaucggagu uagcaaccgg gauuuugugg aaggcauguc ugggggaaca      660

ugggucgaca uugugcugga gcauggcggg ugugugaccg ucauggcuca ggauaagccu      720

acaguugaca ucgagcuugu gaccaccaca guguccaaua uggccgaagu ccgcagcuac      780

ugcuaugagg cuucuauuuc cgauauggcc agcgacucca gaugucccac gcaaggagaa      840

gccuaccugg acaaacaguc ugauacccag uaugugugca agcggacacu cguugacaga      900

ggcuggggca acgggugugg acuguucggc aaagggagcc uggugaccug cgcuaaguuu      960

gcgugcucca agaaaaugac aggaaagagc auccagccug agaaucucga auaccgcauc     1020

augcugucug uccacggcuc ccaacacagc gggaugauug ugaacgauac cggacaugag     1080

acggacgaga aucgggccaa aguugaaauc acaccgaacu cucccagagc ugaggccacc     1140

cuuggcggcu ucgggagccu gggacucgau ugugaaccuc gcaccggccu ggacuuuucc     1200

gaucuuuauu accugacaau gaauaacaag cacuggcucg ugcauaaaga gugguuccac     1260

gacauucccc ugccuuggca cgccggggcu gauaccggaa caccccauug gaauaacaag     1320

gaagcccugg ucgaguuuaa agacgcgcac gcuaagcggc agacgguggu cgugcucggc     1380

ucucaggagg gggccguuca uaccgcccug gcuggcgccc uugaagcuga gauggaugga     1440

gccaagggca gacugagcuc cgggcaccuc aaaugccgcc ugaagaugga caaacuucgg     1500

cugaagggag ugagcuauuc ucucuguaca gcggccuuca ccuuuacaaa aaucccggcu     1560

gaaacccugc auggcacggu caccguggag guucaguacg ccgggacaga cggaccuugc     1620

aaggugcccg cucaaauggc cgucgauaug cagacccuga caccuguggg cagacucauu     1680

accgcgaacc ccgugaucac ggaauccaca gagaauagca agaugaugcu ggagcuugau     1740

cccccuuucg gggacucuua caucguuauu ggcgucggag agaaaaagau cacccaccac     1800

uggcaucgcu ccggcagcac aauugggaaa gccuuugaag cuaccgugcg gggagccaag     1860

agaauggcug ugcugggcga caccgccugg gauuucgggu ccgucggagg cgcccucaac     1920

ucucugggga aaggcaucca ccagauuuuc ggagcugcgu uuaagagccu guucggcggg     1980

auguccuggu uuucucaaau ccuuaucgga acacuccuga uguggcuggg ccucaauacg     2040

aagaacggga gcauuucccu gaugugucuu gcccugggag gcgugcucau cuuccugagc     2100

accgcuguuu cugccgacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8860
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8860
gggagaaagc uuaccauggc cagaaaggag aaaaagcggc gcggcgcuga caccagcgug       60

gggaucgucg gacugcuccu gacaacggcc auggcggcug aaguuaccag acggggcucc      120

gccuacuaua uguaccuuga ucgcaacgac gccggggagg cuauuucuuu ccccacaacc      180

cugggaauga auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca      240

augagcuacg aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg      300

ugcaacacca cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu      360

agacggucuc gcagagccgu uacccuuccc agccauucca cacggaagcu gcaaacccgc      420

ucucagacau ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaauugg      480

auuuuucgca acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc      540

agcacgucuc agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc      600

auuagaugca ucggaguuag caaucgggau uuuguggaag gcaugucugg gggaacaugg      660

gucgacauug ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca      720

guugacaucg agcuugugac caccacagug uccaacaugg ccgaaguccg cagcuacugc      780

uaugaggcuu cuauuuccga uauggccagc gacuccagau gucccacgca aggagaagcc      840

uaccuggaca aacagucuga uacccaguau gugugcaagc ggacacucgu ugacagaggc      900

uggggcaaug gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg      960

ugcuccaaga aaaugacagg aaagagcauc cagccugaga accucgaaua ccgcaucaug     1020

cugucugucc acggcuccca acacagcggg augauuguga augauaccgg acaugagacg     1080

gacgagaacc gggccaaagu ugaaaucaca cccaauucuc ccagagcuga ggccacccuu     1140

ggcggcuucg ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau     1200

cuuuauuacc ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac     1260

auuccccugc cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa     1320

gcccuggucg aguuuaaaga cgcgcacgcu aagcggcaga cgguggucgu gcucggcucu     1380

caggaggggg ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc     1440

aagggcagac ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa     1560

acccugcaug gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag     1620

gugcccgcuc aaauggccgu cgauaugcag acccugacac cugugggcag acucauuacc     1680

gcgaaccccg ugaucacgga auccacagag aacagcaaga ugaugcugga gcuugacccg     1740

ccuuucgggg auucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaagcc uuugaagcua ccgugcgggg agccaagaga     1860

auggcugugc ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaauucu     1920

cuggggaaag gcauccacca gauuuucgga gcugcguuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag     2040

aaugggagca uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc     2100

gcuguuucug ccgacguggg gugcugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8861
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8861
gggagaaagc uuaccaugag aaaggagaaa aagcggcgcg gcgccgacac cagcgugggg       60

aucgucggac ugcuccugac aacggcuaug gccgcggaag uuaccagacg gggcuccgcu      120

uacuauaugu accuugaucg caacgacgcc ggggaggcca uuucuuuccc cacaacccug      180

ggaaugaaua aaugcuauau ccagauuaug gaucucggcc acauguguga cgcuacaaug      240

agcuacgaau gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc      300

aacaccacau ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggccaga      360

cggucucgca gagcuguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu      420

cagacauggc ucgagagcag agaauacacc aagcaccuga uccgggugga gaauuggauu      480

uuucgcaacc cuggcuucgc ccuggcggcc gcugccaucg cuuggcuccu gggguccagc      540

acgucucaga aagucaucua ucuugugaug auucugcuca ucgcccccgc guacuccauu      600

agaugcaucg gaguuagcaa ucgggauuuu guggaaggca ugucuggggg aacauggguc      660

gacauugugc uggagcaugg cgggugugug accgucaugg cccaggauaa gccuacaguu      720

gacaucgagc uugugaccac cacagugucc aacauggcug aaguccgcag cuacugcuau      780

gaggccucua uuuccgauau ggcuagcgac uccagauguc ccacgcaagg agaagccuac      840

cuggacaaac agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg      900

ggcaaugggu guggacuguu cggcaaaggg agccugguga ccugcgccaa guuugcuugc      960

uccaagaaaa ugacaggaaa gagcauccag ccugagaacc ucgaauaccg caucaugcug     1020

ucuguccacg gcucccaaca cagcgggaug auugugaaug auaccggaca ugagacggac     1080

gagaaccggg cgaaaguuga aaucacaccc aauucuccca gagccgaggc uacccuuggc     1140

ggcuucggga gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu     1200

uauuaccuga caaugaacaa uaagcacugg cucgugcaua aagagugguu ccacgacauu     1260

ccccugccuu ggcacgccgg ggccgauacc ggaacacccc auuggaacaa uaaggaagcu     1320

cuggucgagu uuaaagacgc ccacgcgaag cggcagacgg uggucgugcu cggcucucag     1380

gagggggcug uucauaccgc ccuggccggc gcucuugaag ccgagaugga uggagcuaag     1440

ggcagacuga gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag     1500

ggagugagcu auucucucug uacagccgcg uucaccuuua caaaaauccc ggccgaaacc     1560

cugcauggca cggucaccgu ggagguucag uacgcuggga cagacggacc uugcaaggug     1620

cccgcccaaa uggcugucga uaugcagacc cugacaccug ugggcagacu cauuaccgcc     1680

aaccccguga ucacggaauc cacagagaac agcaagauga ugcuggagcu ugacccgccu     1740

uucggggauu cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau     1800

cgcuccggca gcacaauugg gaaagcguuu gaagccaccg ugcggggagc uaagagaaug     1860

gccgugcugg gcgacaccgc uugggauuuc ggguccgucg gaggcgcccu caauucucug     1920

gggaaaggca uccaccagau uuucggagcc gcuuuuaaga gccuguucgg cgggaugucc     1980

ugguuuucuc aaauccuuau cggaacacuc cugauguggc ugggccucaa cacgaagaau     2040

gggagcauuu cccugaugug ucuugcgcug ggaggcgugc ucaucuuccu gagcaccgcc     2100

guuucugcug acguggggug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8862
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8862
gggagaaagc uuaccaugaa ggagaaaaag agacggggcg ccgacaccag cguggggauc       60

gucggacugc uccugacaac ggcuauggcc gcggaaguua cccgcagagg cuccgcuuac      120

uauauguacc uugaucggaa cgacgccggg gaggccauuu cuuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcccgcaga      360

ucucggcgcg cuguuacccu ucccagccau uccacaagaa agcugcaaac ccggucucag      420

acauggcucg agagccgcga auacaccaag caccugauca gaguggagaa uuggauuuuu      480

cggaacccug gcuucgcccu ggcggccgcu gccaucgcuu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cuccauucgc      600

ugcaucggag uuagcaauag agauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggccc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaac auggcugaag uccggagcua cugcuaugag      780

gccucuauuu ccgauauggc uagcgacucc cgcuguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg gggcuggggc      900

aaugggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaccucg aauacagaau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaaugaua ccggacauga gacggacgag     1080

aaccgcgcga aaguugaaau cacacccaau ucuccccggg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu agaaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagcgc cagacggugg ucgugcucgg cucucaggag     1380

ggggcuguuc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cggcugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccggcucau uaccgccaac     1680

cccgugauca cggaauccac agagaacagc aagaugaugc uggagcuuga cccgccuuuc     1740

ggggauucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcauaga     1800

uccggcagca caauugggaa agcguuugaa gccaccgugc gcggagcuaa gcggauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccgucggag gcgcccucaa uucucugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugcugacg uggggugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8863
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8863
gggagaaagc uuaccaugga gaagaaaaga cggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc uauggccgcg gaaguuaccc gcagaggcuc cgcuuacuau      120

auguaccuug aucggaacga cgccggggag gccauuucuu uccccacaac ccugggaaug      180

aauaagugcu auauccagau uauggaucuc ggccacaugu gugacgcuac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaacacc      300

acauccaccu gggucgugua uggaacgugu caucacaaaa agggcgaggc ccgcagaucu      360

cggcgcgcug uuacccuucc cagccauucc acaagaaaac ugcaaacccg gucucagaca      420

uggcucgaga gccgcgaaua caccaagcac cugaucagag uggagaauug gauuuuucgg      480

aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc uccugggguc cagcacgucu      540

cagaagguca ucuaucuugu gaugauucug cucaucgccc ccgcguacuc cauucgcugc      600

aucggaguua gcaauagaga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcccagg auaaaccuac aguugacauc      720

gagcuuguga ccaccacagu guccaacaug gcugaagucc ggagcuacug cuaugaggcc      780

ucuauuuccg auauggcuag cgacucccgc ugucccacgc aaggagaagc cuaccuggac      840

aagcagucug auacccagua ugugugcaaa agaacacucg uugaccgggg cuggggcaau      900

ggguguggac uguucggcaa ggggagccug gugaccugcg ccaaauuugc uugcuccaag      960

aagaugacag gaaaaagcau ccagccugag aaccucgaau acagaaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aaugauaccg gacaugagac ggacgagaac     1080

cgcgcgaagg uugaaaucac acccaauucu ccccgggccg aggcuacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccuaga accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga acaauaaaca cuggcucgug cauaaggagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggccga uaccggaaca ccccauugga acaauaaaga agcucugguc     1320

gaguuuaagg acgcccacgc gaaacgccag acgguggucg ugcucggcuc ucaggagggg     1380

gcuguucaua ccgcccuggc cggcgcucuu gaagccgaga uggauggagc uaagggccgg     1440

cugagcuccg ggcaccucaa gugcagacug aaaauggaca aacuucgccu gaagggagug     1500

agcuauucuc ucuguacagc cgcguucacc uuuacaaaga ucccggccga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcu gggacagacg gaccuugcaa agugcccgcc     1620

caaauggcug ucgauaugca gacccugaca ccugugggcc ggcucauuac cgccaacccc     1680

gugaucacgg aauccacaga gaacagcaag augaugcugg agcuugaccc gccuuucggg     1740

gauucuuaca ucguuauugg cgucggagaa aagaaaauca cccaccacug gcauagaucc     1800

ggcagcacaa uugggaaggc guuugaggcc accgugcgcg gagcuaaacg gauggccgug     1860

cugggcgaca ccgcuuggga uuucgggucc gucggaggcg cccucaauuc ucuggggaag     1920

ggcauccacc agauuuucgg agccgcuuuu aaaagccugu ucggcgggau guccugguuu     1980

ucucaaaucc uuaucggaac acuccugaug uggcugggcc ucaacacgaa gaaugggagc     2040

auuucccuga ugugucuugc gcugggaggc gugcucaucu uccugagcac cgccguuucu     2100

gcugacgugg ggugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8864
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8864
gggagaaagc uuaccaugaa gaaaagacgg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuga caacggcuau ggccgcggag guuacccgca gaggcuccgc uuacuauaug      120

uaccuugauc ggaacgacgc cggggaagcc auuucuuucc ccacaacccu gggaaugaau      180

aagugcuaua uccagauuau ggaucucggc cacaugugug acgcuacaau gagcuacgag      240

ugcccuaugc uggaugaagg gguggagccg gacgaugugg acuguuggug caacaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaaaaagg gcgaagcccg cagaucucgg      360

cgcgcuguua cccuucccag ccauuccaca agaaaacugc aaacccgguc ucagacaugg      420

cucgagagcc gcgaguacac caagcaccug aucagagugg aaaauuggau uuuucggaac      480

ccuggcuucg cccuggcggc cgcugccauc gcuuggcucc ugggguccag cacgucucag      540

aaggucaucu aucuugugau gauucugcuc aucgcccccg cguacuccau ucgcugcauc      600

ggaguuagca auagagauuu uguggagggc augucugggg gaacaugggu cgacauugug      660

cuggaacaug gcgggugugu gaccgucaug gcccaggaua aaccuacagu ugacaucgag      720

cuugugacca ccacaguguc caacauggcu gagguccgga gcuacugcua ugaagccucu      780

auuuccgaua uggcuagcga cucccgcugu cccacgcaag gagaggccua ccuggacaag      840

cagucugaua cccaguaugu gugcaaaaga acacucguug accggggcug gggcaauggg      900

uguggacugu ucggcaaggg gagccuggug accugcgcca aauuugcuug cuccaagaag      960

augacaggaa aaagcaucca gccugaaaac cucgaguaca gaaucaugcu gucuguccac     1020

ggcucccaac acagcgggau gauugugaau gauaccggac augaaacgga cgagaaccgc     1080

gcgaagguug agaucacacc caauucuccc cgggccgaag cuacccuugg cggcuucggg     1140

agccugggac ucgauuguga gccuagaacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaca auaaacacug gcucgugcau aaggaauggu uccacgacau uccccugccu     1260

uggcacgccg gggccgauac cggaacaccc cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggacg cccacgcgaa acgccagacg guggucgugc ucggcucuca ggagggggcu     1380

guucauaccg cccuggccgg cgcucuugag gccgaaaugg auggagcuaa gggccggcug     1440

agcuccgggc accucaagug cagacugaaa auggacaaac uucgccugaa gggagugagc     1500

uauucucucu guacagccgc guucaccuuu acaaagaucc cggccgagac ccugcauggc     1560

acggucaccg uggaaguuca guacgcuggg acagacggac cuugcaaagu gcccgcccaa     1620

auggcugucg auaugcagac ccugacaccu gugggccggc ucauuaccgc caaccccgug     1680

aucacggagu ccacagaaaa cagcaagaug augcuggagc uugacccgcc uuucggggau     1740

ucuuacaucg uuauuggcgu cggagagaag aaaaucaccc accacuggca uagauccggc     1800

agcacaauug ggaaggcguu ugaagccacc gugcgcggag cuaaacggau ggccgugcug     1860

ggcgacaccg cuugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaagggc     1920

auccaccaga uuuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu     2040

ucccugaugu gucuugcgcu gggaggcgug cucaucuucc ugagcaccgc cguuucugcu     2100

gacguggggu gcugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8865
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8865
gggagaaagc uuaccaugaa gagacggggc gccgacacca gcguggggau cgucggacug       60

cuccugacaa cggcuauggc cgcggagguu acccgcagag gcuccgcuua cuauauguac      120

cuugaucgga acgacgccgg ggaagccauu ucuuucccca caacccuggg aaugaauaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg cuacaaugag cuacgagugc      240

ccuaugcugg augaaggggu ggagccggac gauguggacu guuggugcaa caccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aagcccgcag aucucggcgc      360

gcuguuaccc uucccagcca uuccacaaga aagcugcaaa cccggucuca gacauggcuc      420

gagagccgcg aguacaccaa acaccugauc agaguggaaa auuggauuuu ucggaacccu      480

ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaag      540

gucaucuauc uugugaugau ucugcucauc gcccccgcgu acuccauucg cugcaucgga      600

guuagcaaua gagauuuugu ggagggcaug ucugggggaa caugggucga cauugugcug      660

gaacauggcg ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa cauggcugag guccggagcu acugcuauga agccucuauu      780

uccgauaugg cuagcgacuc ccgcuguccc acgcaaggag aggccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagagaaca cucguugacc ggggcugggg caaugggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caaaaagaug      960

acaggaaaga gcauccagcc ugaaaaccuc gaguacagaa ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaaugau accggacaug aaacggacga gaaccgcgcg     1080

aaaguugaga ucacacccaa uucuccccgg gccgaagcua cccuuggcgg cuucgggagc     1140

cugggacucg auugugagcc uagaaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaacaaua agcacuggcu cgugcauaaa gaaugguucc acgacauucc ccugccuugg     1260

cacgccgggg ccgauaccgg aacaccccau uggaacaaua aggaggcucu ggucgaauuu     1320

aaagacgccc acgcgaagcg ccagacggug gucgugcucg gcucucagga gggggcuguu     1380

cauaccgccc uggccggcgc ucuugaggcc gaaauggaug gagcuaaagg ccggcugagc     1440

uccgggcacc ucaagugcag acugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucucucugua cagccgcguu caccuuuaca aaaaucccgg ccgagacccu gcauggcacg     1560

gucaccgugg aaguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug     1620

gcugucgaua ugcagacccu gacaccugug ggccggcuca uuaccgccaa ccccgugauc     1680

acggagucca cagaaaacag caaaaugaug cuggagcuug acccgccuuu cggggauucu     1740

uacaucguua uuggcgucgg agagaagaag aucacccacc acuggcauag auccggcagc     1800

acaauuggga aagcguuuga agccaccgug cgcggagcua agcggauggc cgugcugggc     1860

gacaccgcuu gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaca cgaaaaaugg gagcauuucc     2040

cugauguguc uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac     2100

guggggugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8866
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8866
gggagaaagc uuaccaugag acggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg cuauggccgc ggagguuacc cgcagaggcu ccgcuuacua uauguaccuu      120

gaucggaacg acgccgggga agccauuucu uuccccacaa cccugggaau gaauaagugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcua caaugagcua cgagugcccu      240

augcuggaug aaggggugga gccggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaaa aagggcgaag cccgcagauc ucggcgcgcu      360

guuacccuuc ccagccauuc cacaagaaaa cugcaaaccc ggucucagac auggcucgag      420

agccgcgagu acaccaagca ccugaucaga guggaaaauu ggauuuuucg gaacccuggc      480

uucgcccugg cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu      600

agcaauagag auuuugugga gggcaugucu gggggaacau gggucgacau ugugcuggaa      660

cauggcgggu gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggcugagguc cggagcuacu gcuaugaagc cucuauuucc      780

gauauggcua gcgacucccg cugucccacg caaggagagg ccuaccugga caagcagucu      840

gauacccagu augugugcaa aagaacacuc guugaccggg gcuggggcaa ugggugugga      900

cuguucggca aggggagccu ggugaccugc gccaaauuug cuugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga aaaccucgag uacagaauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaaa cggacgagaa ccgcgcgaag     1080

guugagauca cacccaauuc uccccgggcc gaagcuaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugagccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccccu gccuuggcac     1260

gccggggccg auaccggaac accccauugg aacaauaaag aggcucuggu cgaauuuaag     1320

gacgcccacg cgaaacgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau     1380

accgcccugg ccggcgcucu ugaggccgaa auggauggag cuaagggccg gcugagcucc     1440

gggcaccuca aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu     1500

cucuguacag ccgcguucac cuuuacaaag aucccggccg agacccugca uggcacgguc     1560

accguggaag uucaguacgc ugggacagac ggaccuugca aagugcccgc ccaaauggcu     1620

gucgauaugc agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca     1800

auugggaagg cguuugaagc caccgugcgc ggagcuaaac ggauggccgu gcugggcgac     1860

accgcuuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa gggcauccac     1920

cagauuuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacgug     2100

gggugcugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8867
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8867
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga gguuacccgg cgcggcuccg cuuacuauau guaccuugau      120

agaaacgacg ccggggaagc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga gugcccuaug      240

cuggaugaag ggguggagcc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaagccc ggcgcucuag acgggcuguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgagagc      420

cgggaguaca ccaagcaccu gauccgcgug gaaaauugga uuuuuagaaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaggg caugucuggg ggaacauggg ucgacauugu gcuggaacau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaggucaga agcuacugcu augaagccuc uauuuccgau      780

auggcuagcg acucccggug ucccacgcaa ggagaggccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugaaaa ccucgaguac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugaaacgg acgagaacag agcgaagguu     1080

gagaucacac ccaauucucc ccgcgccgaa gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug agccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggaaugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaagagg cucuggucga auuuaaggac     1320

gcccacgcga aaagacagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga ggccgaaaug gauggagcua agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaagauc ccggccgaga cccugcaugg cacggucacc     1560

guggaaguuc aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg ccaaccccgu gaucacggag     1680

uccacagaaa acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggcgu uugaagccac cgugagagga gcuaaacgca uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacgugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8868
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8868
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaggu uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

aacgacgccg gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug      240

gaugaagggg uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caaaaagggc gaagccagac ggucucgcag agcuguuacc      360

cuucccagcc auuccacacg gaaacugcaa acccgcucuc agacauggcu cgagagcaga      420

gaguacacca agcaccugau ccggguggaa aauuggauuu uucgcaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauua gaugcaucgg aguuagcaau      600

cgggauuuug uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga gguccgcagc uacugcuaug aagccucuau uuccgauaug      780

gcuagcgacu ccagaugucc cacgcaagga gaggccuacc uggacaagca gucugauacc      840

caguaugugu gcaaacggac acucguugac cgcggcuggg gcaaugggug uggacuguuc      900

ggcaagggga gccuggugac cugcgccaaa uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gaaacggacg agaaccgggc gaagguugag     1080

aucacaccca auucucccag agccgaagcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugagc cucgcaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc     1320

cacgcgaaac ggcagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaggc cgaaauggau ggagcuaagg gcagacugag cuccgggcac     1440

cucaaaugcc gccugaagau ggacaaacuu cggcugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug     1560

gaaguucagu acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggcagacuc auuaccgcca accccgugau cacggagucc     1680

acagaaaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcauc gcuccggcag cacaauuggg     1800

aaggcguuug aagccaccgu gcggggagcu aaaagaaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu     1920

uucggagccg cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga cguggggugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8869
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8869
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccugacaacg       60

gcuauggccg cggagguuac cagacgggga uccgcuuacu auauguaccu ugaucgcaac      120

gacgccggcg aagccauuuc uuuccccaca acccugggga ugaauaagug cuauauccag      180

auuauggauc ucggacacau gugugacgcu acaaugagcu acgagugccc uaugcuggau      240

gaaggcgugg agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uaugggacgu gucaucacaa aaagggagaa gccagacggu cucgcagagc uguuacccuu      360

cccagccauu ccacacggaa acugcaaacc cgcucucaga cauggcucga gagcagagag      420

uacaccaagc accugauccg gguggaaaau uggauuuuuc gcaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggc uccagcacgu cucagaaagu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauuagau gcaucggggu uagcaaucgg      600

gauuuugugg agggaauguc uggcgggaca ugggucgaca uugugcugga acauggaggc      660

ugugugaccg ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaggu ccgcagcuac ugcuaugaag ccucuauuuc cgauauggcu      780

agcgacucca gaugucccac gcaaggggag gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aacggacacu cguugaccgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucca agaaaaugac aggaaagagc      960

auccagccug aaaaccucga guacagaauc augcugucug uccacggguc ccaacacagc     1020

ggcaugauug ugaaugauac cgggcaugaa acggacgaga accgggcgaa gguugagauc     1080

acacccaauu cucccagagc cgaagcuacc cuuggaggcu ucggcagccu ggggcucgau     1140

ugugagccuc gcaccggacu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga augguuccac gacauucccc ugccuuggca cgccggcgcc     1260

gauaccggga caccccauug gaacaauaaa gaggcucugg ucgaauuuaa ggacgcccac     1320

gcgaaacggc agacgguggu cgugcucgga ucucaggagg gcgcuguuca uaccgcccug     1380

gccggggcuc uugaggccga aauggauggc gcuaagggaa gacugagcuc cggccaccuc     1440

aaaugccgcc ugaagaugga caagcugcgg cugaaagggg ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gagacccugc auggaacggu caccguggaa     1560

guucaguacg cuggcacaga cgggccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg aagacucauu accgccaacc ccgugaucac ggaguccaca     1680

gaaaacagca agaugaugcu ggagcuugac ccgccuuucg gcgauucuua caucguuauu     1740

ggggucggcg agaaaaagau cacccaccac uggcaucgcu ccggaagcac aauuggcaag     1800

gcguuugaag ccaccgugag aggggcuaaa cggauggccg ugcugggaga caccgcuugg     1860

gauuucggcu ccgucggggg agcccucaau ucucugggca aggggaucca ccagauuuuc     1920

ggcgccgcuu uuaaaagccu guucggaggc auguccuggu uuucucaaau ccuuaucggg     1980

acacuccuga uguggcuggg acucaacacg aagaauggca gcauuucccu gaugugucuu     2040

gcgcuugggg gagugcucau cuuccugagc accgccguuu cugcugacgu gggcugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8870
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8870
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu gacaacggcc       60

auggcugccg agguuaccag acggggaucc gcguacuaua uguaccuuga ucgcaacgac      120

gcuggcgaag ccauuucuuu ccccacaacc cuggggauga auaagugcua uauccagauu      180

auggaucucg gacacaugug ugacgccaca augagcuacg agugcccuau gcuggaugaa      240

ggcguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

gggacguguc aucacaaaaa gggagaagcu agacggucuc gcagagccgu uacccuuccc      360

agccauucca cacggaaacu gcaaacccgc ucucagacau ggcucgagag cagagaguac      420

accaagcacc ugauccgggu ggaaaauugg auuuuucgca acccuggcuu cgcucuggcc      480

gcggccgcua ucgccuggcu ccugggcucc agcacgucuc agaaagucau cuaucuugug      540

augauucugc ucaucgcucc cgccuacucc auuagaugca ucgggguuag caaucgggau      600

uuuguggagg gaaugucugg cgggacaugg gucgacauug ugcuggaaca uggaggcugu      660

gugaccguca uggcgcagga uaagccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg ccgagguccg cagcuacugc uaugaagcua gcauuuccga uauggccagc      780

gacuccagau gucccacgca aggggaggcu uaccuggaca agcagucuga uacccaguau      840

gugugcaaac ggacacucgu ugaccgcgga uggggcaaug gcugugggcu guucggaaag      900

ggcagccugg ugaccugcgc caaauuugcc ugcuccaaga aaaugacagg gaagucuauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggauccca acacagcggc     1020

augauuguga augauaccgg gcaugaaacg gacgagaacc gggcuaaggu ugagaucaca     1080

cccaauucuc ccagagcgga agccacccuu ggaggcuucg gcagccuggg gcucgauugu     1140

gagccucgca ccggacugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc uggcgccgau     1260

accgggacac cccauuggaa caauaaagag gcccuggucg aauuuaagga cgcucacgcc     1320

aaacggcaga cgguggucgu gcucggaucu caggagggcg cgguucauac cgcucuggcc     1380

ggggcccuug aggcugaaau ggauggcgcc aagggaagac ugagcuccgg ccaccucaaa     1440

ugccgccuga agauggacaa gcugcggcug aaagggguga gcuauucucu cuguacagcu     1500

gccuucaccu uuacaaagau cccggcggag acccugcaug gaacggucac cguggaaguu     1560

caguacgccg gcacagacgg gccuugcaaa gugcccgcuc aaauggccgu cgauaugcag     1620

acccugacac cugugggaag acucauuacc gcuaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucggcg auucuuacau cguuauuggg     1740

gucggcgaga aaaagaucac ccaccacugg caucgcuccg gaagcacaau uggcaaggcc     1800

uuugaagcga ccgugagagg ggccaaacgg auggcugugc ugggagacac cgccugggau     1860

uucggcuccg ucgggggagc ucucaauucu cugggcaagg ggauccacca gauuuucggc     1920

gccgccuuua aaagccuguu cggaggcaug uccugguuuu cucaaauccu uaucgggaca     1980

cuccugaugu ggcugggacu caacacgaag aauggcagca uuucccugau gugucuugcu     2040

cuugggggag ugcucaucuu ccugagcacc gcgguuucug ccgacguggg cugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8871
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8871
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccg augugggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8872
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8872
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgcgg uuaccagacg gggauccgcu uacuauaugu accuugaccg caacgaugcc      120

ggcgaggcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugcuacaaug agcuacgaau gcccuaugcu ggacgagggc      240

guggaaccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaggccaga cggucucgca gagcuguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgaaagcag agaguacacc      420

aagcaccuga uccgggugga gaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggaaggaa ugucuggcgg gacauggguc gauauugugc uggagcaugg aggcugugug      660

accgucaugg cccaggacaa gccuacaguu gauaucgaac uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaggccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaagccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauccag      960

ccugagaacc ucgaauacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugagacggau gaaaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaggc uacccuugga ggcuucggca gccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccccugccuu ggcacgccgg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaagcu cuggucgagu uuaaggaugc ccacgcgaaa     1320

cggcagacgg uggucgugcu cggaucucag gaaggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgagaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgaaacc cugcauggaa cggucaccgu ggagguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaggccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcug augugggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8873
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8873
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcggc uguuacccgg cgcggcuccg ccuacuauau guaccuugau      120

agaaacgacg ccggggaggc uauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgccacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaggcuc ggcgcucuag acgggccguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgaaagc      420

cgggaguaca ccaagcaccu gauccgcgug gagaauugga uuuuuagaaa cccuggcuuc      480

gcgcuggccg cugccgcuau cgccuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgcgccc gccuacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcucaggau aagccuacag uugacaucga acuugugacc      720

accacagugu ccaacauggc cgaggucaga agcuacugcu augaggcuuc uauuuccgau      780

auggccagcg acucccggug ucccacgcaa ggagaagccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcu aaauuugcgu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgaaaacag agccaagguu     1080

gagaucacac ccaauucucc ccgcgcugag gccacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggcugaua ccggaacacc ccauuggaac aauaaagaag cccuggucga guuuaaggac     1320

gcgcacgcua aaagacagac gguggucgug cucggcucuc aggaaggggc cguucauacc     1380

gcccuggcug gcgcccuuga ggcugagaug gauggagcca agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagcgg ccuucaccuu uacaaagauc ccggcugaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgccgg gacagacgga ccuugcaaag ugcccgcuca aauggccguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg cgaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggaa cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggccu uugaggcuac cgugagagga gccaaacgca uggcugugcu gggcgacacc     1860

gccugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag cugcguuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugccc ugggaggcgu gcucaucuuc cugagcaccg cuguuucugc cgacgugggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8874
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8874
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gaggcguuac gcggcgcggg      120

agcgccuacu auauguaccu ggauagaaac gacgcuggag aggccauuuc cuuucccacc      180

acacuuggca ugaauaaaug cuauauccaa auuauggauc uggggcauau gugugacgcg      240

accaugucuu acgaaugccc uaugcucgau gagggagugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggcaccu gucaccauaa gaaaggcgag      360

gcucggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg guuugcccuu gcugccgcug ccaucgcgug gcugcucgga      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ccccgcuuac      600

ucuauucggu gcaucggcgu uuccaaucgc gauuucgugg aagggaugag cggaggcacg      660

ugggucgaca uugugcucga gcacggggga ugugugacag ucauggccca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggcugaggu cagauccuac      780

ugcuaugagg ccagcauuuc cgauauggcg ucugacagcc ggugucccac acagggcgaa      840

gccuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggga auggaugugg ccuguuuggg aaaggaagcc uugugacaug cgcuaaguuc      960

gccugcucua aaaagaugac cggcaagucc auccaaccug agaaccugga auaccggauc     1020

augcucagcg uccacggguc ucagcauagc ggaaugauug ugaaugauac aggccacgag     1080

accgacgaaa acagagcuaa aguugagauc acgcccaauu ccccgcgcgc cgaggccaca     1140

cugggcgggu uuggaucucu uggccuggau ugugaaccuc ggaccgggcu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcuggagcg gauacaggca caccccacug gaacaauaag     1320

gaagcccuug ucgaguucaa agacgcucau gccaagagac agacaguggu cgugcugggg     1380

ucccaggaag gcgccguuca cacggcucuc gccggagcgc uggaggcuga gauggauggc     1440

gccaaagggc gccuuagcuc uggacaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggcg uguccuauag ccucuguacc gccgcuuuua cauucaccaa aaucccggcc     1560

gaaacacugc acgggaccgu cacgguggag guucaauacg cuggaaccga cggcccuugc     1620

aaggugcccg cccagauggc ggucgauaug cagacacuua ccccuguggg gcgccugauu     1680

acagccaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gcgacagcua caucguuauu ggagucggcg agaagaagau cacacaccau     1800

uggcaccggu ccgggucuac cauuggaaag gcuuucgagg ccacagugag aggcgcuaaa     1860

cgcauggccg ugcuggggga caccgcgugg gauuucggaa gcgucggcgg ggcccuuaau     1920

ucccucggca aaggaaucca ucaaauuuuu ggcgcugccu ucaagucucu guuuggggga     1980

augagcuggu ucucccagau ccugaucggc acccuccuga uguggcuugg gcugaacacc     2040

aaaaauggaa gcauuucucu caugugucug gcucugggcg gggugcuuau cuuucucucc     2100

acggccguua gcgccgacgu gggcugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8875
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8875
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gagccguuac gcggcgcggc      120

agcgcuuacu auauguaccu ggauagaaac gacgccgggg aggcgauuuc cuuucccacc      180

acacuuggaa ugaauaaaug cuauauccaa auuauggauc ugggccauau gugugacgcu      240

accaugucuu acgaaugccc uaugcucgau gagggggugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggaaccu gucaccauaa gaaaggcgag      360

gcccggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg cuuugcucuu gccgcugccg cgaucgccug gcugcucggg      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ucccgccuac      600

ucuauucggu gcaucggagu uuccaaucgc gauuucgugg aaggcaugag cgggggaacg      660

ugggucgaca uugugcucga gcacggcggg ugugugacag ucauggcuca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggccgaggu cagauccuac      780

ugcuaugagg cgagcauuuc cgauauggcc ucugacagcc ggugucccac acagggagag      840

gcuuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggca augggugugg acuguuuggc aaagggagcc uugugacaug cgccaaguuc      960

gcuugcucua aaaagaugac cggaaagucc auccaaccug aaaaccugga auaccggauc     1020

augcucagcg uccacggcuc ucagcauagc gggaugauug ugaaugauac aggacacgag     1080

accgacgaaa acagagccaa aguugagauc acgcccaauu ccccgcgcgc cgaggcuaca     1140

cugggcggcu uugggucucu uggacuggau ugugaaccuc ggaccggccu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcgggggcc gauacaggaa caccccacug gaacaauaag     1320

gaagcucuug ucgaguucaa agacgcccau gccaagagac agacaguggu cgugcugggc     1380

ucccaggaag gggcuguuca cacggcccuc gcgggcgcuc uggaggccga gauggaugga     1440

gccaaaggcc gccuuagcuc ugggcaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggag uguccuauag ccucuguacc gcugccuuua cauucaccaa aaucccggcu     1560

gaaacacugc acggcaccgu cacgguggag guucaauacg ccgggaccga cggaccuugc     1620

aaggugcccg cgcagauggc cgucgauaug cagacacuua ccccuguggg ccgccugauu     1680

acagcuaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gggacagcua caucguuauu ggcgucggag agaagaagau cacacaccau     1800

uggcaccggu ccggcucuac cauugggaaa gccuucgagg cuacagugag aggagccaag     1860

cgcauggcgg ugcugggcga caccgccugg gauuucggga gcgucggagg cgcucuuaau     1920

ucccucggga aaggcaucca ucaaauuuuu ggagccgcuu ucaagucucu guuuggcggg     1980

augagcuggu ucucccagau ccugaucgga acccuccuga uguggcuugg ccugaacacc     2040

aaaaauggga gcauuucucu caugugucug gcccugggag gcgugcuuau cuuucucucc     2100

acggccguua gcgcugacgu ggggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8876
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8876
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  8877
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8877
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagcggcg cggcgcugac       60

accagcgugg ggaucgucgg acugcuccug acaacggcca uggcggcuga aguuaccaga      120

cggggcuccg ccuacuauau guaccuugau cgcaaugacg ccggggaggc uauuucuuuc      180

cccacaaccc ugggaaugaa caaaugcuau auccagauua uggaucucgg ccacaugugu      240

gacgccacaa ugagcuacga augcccuaug cuggaugagg ggguggaacc ggacgaugug      300

gacuguuggu gcaauaccac auccaccugg gucguguaug gaacguguca ucacaagaaa      360

ggcgaggcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      420

caaacccgcu cucagacaug gcucgagagc agagaauaca ccaagcaccu gauccgggug      480

gagaacugga ucuuucgcaa uccuggcuuc gcgcuggccg cugccgcuau ugccuggcuc      540

cuggggucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcgccc      600

gccuacucca uuagaugcau cggaguuagc aaccgggauu uuguggaagg caugucuggg      660

ggaacauggg ucgacauugu gcuggagcau ggcgggugug ugaccgucau ggcucaggau      720

aagccuacag uugacaucga gcuugugacc accacagugu ccaauauggc cgaaguccgc      780

agcuacugcu augaggcuuc uaucuccgau auggccagcg acuccagaug ucccacgcaa      840

ggagaagccu accuggacaa acagucugau acccaguaug ugugcaagcg gacacucguu      900

gacagaggcu ggggcaacgg guguggacug uucggcaaag ggagccuggu gaccugcgcu      960

aaguuugcgu gcuccaagaa aaugacagga aagagcauuc agccugagaa ucucgaauac     1020

cgcaucaugc ugucugucca cggcucccaa cacagcggga ugauugugaa cgauaccgga     1080

caugagacgg acgagaaucg ggccaaaguu gaaaucacac cgaacucucc cagagcugag     1140

gccacccuug gcggcuucgg gagccuggga cucgauugug aaccucgcac cggccuggac     1200

uuuuccgauc uuuauuaccu gacaaugaau aacaagcacu ggcucgugca uaaagagugg     1260

uuccacgaca uuccccugcc uuggcacgcc ggggcugaua ccggaacacc ccauuggaau     1320

aacaaggaag cccuggucga guuuaaagac gcgcacgcua agcggcagac gguggucgug     1380

cucggcucuc aggagggggc cguucauacc gcccuggcug gcgcccuuga agcugagaug     1440

gauggagcca agggcagacu gagcuccggg caccucaaau gccgccugaa gauggacaaa     1500

cuucggcuga agggagugag cuauucucuc uguacagcgg ccuucaccuu uacaaaaauc     1560

ccggcugaaa cccugcaugg cacggucacc guggagguuc aguacgccgg gacagacgga     1620

ccuugcaagg ugcccgcuca aauggccguc gauaugcaga cccugacacc ugugggcaga     1680

cucaucaccg cgaaccccgu gauuacggaa uccacagaga auagcaagau gaugcuggag     1740

cuugaucccc cuuucgggga cucuuacauc guuauuggcg ucggagagaa aaagaucacc     1800

caccacuggc aucgcuccgg cagcacaauu gggaaagccu uugaagcuac cgugcgggga     1860

gccaagagaa uggcugugcu gggcgacacc gccugggauu ucggguccgu cggaggcgcc     1920

cucaacucuc uggggaaagg cauccaccag aucuucggag cugcguuuaa gagccuguuc     1980

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacac uccugaugug gcugggccuc     2040

aauacgaaga acgggagcau uucccugaug ugucuugccc ugggaggcgu gcucaucuuc     2100

cugagcaccg cuguuucugc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8878
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8878
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agcggcgcgg cgcugacacc       60

agcgugggga uugucggacu gcuccugaca acggccaugg cggcugaagu uaccagacgg      120

ggcuccgccu acuauaugua ccuugaucgc aaugacgccg gggaggcuau cucuuucccc      180

acaacccugg gaaugaacaa augcuauauu cagaucaugg aucucggcca caugugugac      240

gccacaauga gcuacgaaug cccuaugcug gaugaggggg uggaaccgga cgauguggac      300

uguuggugca auaccacauc caccuggguc guguauggaa cgugucauca caagaaaggc      360

gaggcuagac ggucucgcag agccguuacc cuucccagcc auuccacacg gaagcugcaa      420

acccgcucuc agacauggcu cgagagcaga gaauacacca agcaccugau ucggguggag      480

aacuggaucu uucgcaaucc uggcuucgcg cuggccgcug ccgcuaucgc cuggcuccug      540

ggguccagca cgucucagaa agucauuuau cuugugauga uccugcucau ugcgcccgcc      600

uacuccauca gaugcauugg aguuagcaac cgggauuuug uggaaggcau gucuggggga      660

acaugggucg acaucgugcu ggagcauggc ggguguguga ccgucauggc ucaggauaag      720

ccuacaguug acauugagcu ugugaccacc acagugucca auauggccga aguccgcagc      780

uacugcuaug aggcuucuau cuccgauaug gccagcgacu ccagaugucc cacgcaagga      840

gaagccuacc uggacaaaca gucugauacc caguaugugu gcaagcggac acucguugac      900

agaggcuggg gcaacgggug uggacuguuc ggcaaaggga gccuggugac cugcgcuaag      960

uuugcgugcu ccaagaaaau gacaggaaag agcauccagc cugagaaucu cgaauaccgc     1020

auuaugcugu cuguccacgg cucccaacac agcgggauga ucgugaacga uaccggacau     1080

gagacggacg agaaucgggc caaaguugaa auuacaccga acucucccag agcugaggcc     1140

acccuuggcg gcuucgggag ccugggacuc gauugugaac cucgcaccgg ccuggacuuu     1200

uccgaucuuu auuaccugac aaugaauaac aagcacuggc ucgugcauaa agagugguuc     1260

cacgacaucc cccugccuug gcacgccggg gcugauaccg gaacacccca uuggaauaac     1320

aaggaagccc uggucgaguu uaaagacgcg cacgcuaagc ggcagacggu ggucgugcuc     1380

ggcucucagg agggggccgu ucauaccgcc cuggcuggcg cccuugaagc ugagauggau     1440

ggagccaagg gcagacugag cuccgggcac cucaaaugcc gccugaagau ggacaaacuu     1500

cggcugaagg gagugagcua uucucucugu acagcggccu ucaccuuuac aaaaauuccg     1560

gcugaaaccc ugcauggcac ggucaccgug gagguucagu acgccgggac agacggaccu     1620

ugcaaggugc ccgcucaaau ggccgucgau augcagaccc ugacaccugu gggcagacuc     1680

aucaccgcga accccgugau cacggaaucc acagagaaua gcaagaugau gcuggagcuu     1740

gaucccccuu ucggggacuc uuacauuguu aucggcgucg gagagaaaaa gauuacccac     1800

cacuggcauc gcuccggcag cacaaucggg aaagccuuug aagcuaccgu gcggggagcc     1860

aagagaaugg cugugcuggg cgacaccgcc ugggauuucg gguccgucgg aggcgcccuc     1920

aacucucugg ggaaaggcau ucaccagauc uucggagcug cguuuaagag ccuguucggc     1980

gggauguccu gguuuucuca aauccuuauu ggaacacucc ugauguggcu gggccucaau     2040

acgaagaacg ggagcaucuc ccugaugugu cuugcccugg gaggcgugcu cauuuuccug     2100

agcaccgcug uuucugccga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8879
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8879
gggagaaagc uuaccaugaa cgccagaaag gagaaaaagc ggcgcggcgc ugacaccagc       60

guggggaucg ucggacugcu ccugacaacg gccauggcgg cugaaguuac cagacggggc      120

uccgccuacu auauguaccu ugaucgcaau gacgccgggg aggcuauuuc uuuccccaca      180

acccugggaa ugaacaaaug cuauauccag auuauggauc ucggccacau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggggugg aaccggacga uguggacugu      300

uggugcaaua ccacauccac cugggucgug uauggaacgu gucaucacaa gaaaggcgag      360

gcuagacggu cucgcagagc cguuacccuu cccagccauu ccacacggaa gcugcaaacc      420

cgcucucaga cauggcucga gagcagagaa uacaccaagc accugauccg gguggagaac      480

uggauuuuuc gcaauccugg cuucgcgcug gccgcugccg cuaucgccug gcuccugggg      540

uccagcacgu cucagaaagu caucuaucuu gugaugauuc ugcucaucgc gcccgccuac      600

uccauuagau gcaucggagu uagcaaccgg gauuuugugg aaggcauguc ugggggaaca      660

ugggucgaca uugugcugga gcauggcggg ugugugaccg ucauggcuca ggauaagccu      720

acaguugaca ucgagcuugu gaccaccaca guguccaaua uggccgaagu ccgcagcuac      780

ugcuaugagg cuucuauuuc cgauauggcc agcgacucca gaugucccac gcaaggagaa      840

gccuaccugg acaaacaguc ugauacccag uaugugugca agcggacacu cguugacaga      900

ggcuggggca acgggugugg acuguucggc aaagggagcc uggugaccug cgcuaaguuu      960

gcgugcucca agaaaaugac aggaaagagc auccagccug agaaucucga auaccgcauc     1020

augcugucug uccacggcuc ccaacacagc gggaugauug ugaacgauac cggacaugag     1080

acggacgaga aucgggccaa aguugaaauc acaccgaacu cucccagagc ugaggccacc     1140

cuuggcggcu ucgggagccu gggacucgau ugugaaccuc gcaccggccu ggacuuuucc     1200

gaucuuuauu accugacaau gaauaacaag cacuggcucg ugcauaaaga gugguuccac     1260

gacauucccc ugccuuggca cgccggggcu gauaccggaa caccccauug gaauaacaag     1320

gaagcccugg ucgaguuuaa agacgcgcac gcuaagcggc agacgguggu cgugcucggc     1380

ucucaggagg gggccguuca uaccgcccug gcuggcgccc uugaagcuga gauggaugga     1440

gccaagggca gacugagcuc cgggcaccuc aaaugccgcc ugaagaugga caaacuucgg     1500

cugaagggag ugagcuauuc ucucuguaca gcggccuuca ccuuuacaaa aaucccggcu     1560

gaaacccugc auggcacggu caccguggag guucaguacg ccgggacaga cggaccuugc     1620

aaggugcccg cucaaauggc cgucgauaug cagacccuga caccuguggg cagacucauu     1680

accgcgaacc ccgugaucac ggaauccaca gagaauagca agaugaugcu ggagcuugau     1740

cccccuuucg gggacucuua caucguuauu ggcgucggag agaaaaagau cacccaccac     1800

uggcaucgcu ccggcagcac aauugggaaa gccuuugaag cuaccgugcg gggagccaag     1860

agaauggcug ugcugggcga caccgccugg gauuucgggu ccgucggagg cgcccucaac     1920

ucucugggga aaggcaucca ccagauuuuc ggagcugcgu uuaagagccu guucggcggg     1980

auguccuggu uuucucaaau ccuuaucgga acacuccuga uguggcuggg ccucaauacg     2040

aagaacggga gcauuucccu gaugugucuu gcccugggag gcgugcucau cuuccugagc     2100

accgcuguuu cugccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8880
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8880
gggagaaagc uuaccauggc cagaaaggag aaaaagcggc gcggcgcuga caccagcgug       60

gggaucgucg gacugcuccu gacaacggcc auggcggcug aaguuaccag acggggcucc      120

gccuacuaua uguaccuuga ucgcaacgac gccggggagg cuauuucuuu ccccacaacc      180

cugggaauga auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca      240

augagcuacg aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg      300

ugcaacacca cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu      360

agacggucuc gcagagccgu uacccuuccc agccauucca cacggaagcu gcaaacccgc      420

ucucagacau ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaauugg      480

auuuuucgca acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc      540

agcacgucuc agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc      600

auuagaugca ucggaguuag caaucgggau uuuguggaag gcaugucugg gggaacaugg      660

gucgacauug ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca      720

guugacaucg agcuugugac caccacagug uccaacaugg ccgaaguccg cagcuacugc      780

uaugaggcuu cuauuuccga uauggccagc gacuccagau gucccacgca aggagaagcc      840

uaccuggaca aacagucuga uacccaguau gugugcaagc ggacacucgu ugacagaggc      900

uggggcaaug gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg      960

ugcuccaaga aaaugacagg aaagagcauc cagccugaga accucgaaua ccgcaucaug     1020

cugucugucc acggcuccca acacagcggg augauuguga augauaccgg acaugagacg     1080

gacgagaacc gggccaaagu ugaaaucaca cccaauucuc ccagagcuga ggccacccuu     1140

ggcggcuucg ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau     1200

cuuuauuacc ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac     1260

auuccccugc cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa     1320

gcccuggucg aguuuaaaga cgcgcacgcu aagcggcaga cgguggucgu gcucggcucu     1380

caggaggggg ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc     1440

aagggcagac ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa     1560

acccugcaug gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag     1620

gugcccgcuc aaauggccgu cgauaugcag acccugacac cugugggcag acucauuacc     1680

gcgaaccccg ugaucacgga auccacagag aacagcaaga ugaugcugga gcuugacccg     1740

ccuuucgggg auucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaagcc uuugaagcua ccgugcgggg agccaagaga     1860

auggcugugc ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaauucu     1920

cuggggaaag gcauccacca gauuuucgga gcugcguuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag     2040

aaugggagca uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc     2100

gcuguuucug ccgacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8881
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8881
gggagaaagc uuaccaugag aaaggagaaa aagcggcgcg gcgccgacac cagcgugggg       60

aucgucggac ugcuccugac aacggcuaug gccgcggaag uuaccagacg gggcuccgcu      120

uacuauaugu accuugaucg caacgacgcc ggggaggcca uuucuuuccc cacaacccug      180

ggaaugaaua aaugcuauau ccagauuaug gaucucggcc acauguguga cgcuacaaug      240

agcuacgaau gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc      300

aacaccacau ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggccaga      360

cggucucgca gagcuguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu      420

cagacauggc ucgagagcag agaauacacc aagcaccuga uccgggugga gaauuggauu      480

uuucgcaacc cuggcuucgc ccuggcggcc gcugccaucg cuuggcuccu gggguccagc      540

acgucucaga aagucaucua ucuugugaug auucugcuca ucgcccccgc guacuccauu      600

agaugcaucg gaguuagcaa ucgggauuuu guggaaggca ugucuggggg aacauggguc      660

gacauugugc uggagcaugg cgggugugug accgucaugg cccaggauaa gccuacaguu      720

gacaucgagc uugugaccac cacagugucc aacauggcug aaguccgcag cuacugcuau      780

gaggccucua uuuccgauau ggcuagcgac uccagauguc ccacgcaagg agaagccuac      840

cuggacaaac agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg      900

ggcaaugggu guggacuguu cggcaaaggg agccugguga ccugcgccaa guuugcuugc      960

uccaagaaaa ugacaggaaa gagcauccag ccugagaacc ucgaauaccg caucaugcug     1020

ucuguccacg gcucccaaca cagcgggaug auugugaaug auaccggaca ugagacggac     1080

gagaaccggg cgaaaguuga aaucacaccc aauucuccca gagccgaggc uacccuuggc     1140

ggcuucggga gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu     1200

uauuaccuga caaugaacaa uaagcacugg cucgugcaua aagagugguu ccacgacauu     1260

ccccugccuu ggcacgccgg ggccgauacc ggaacacccc auuggaacaa uaaggaagcu     1320

cuggucgagu uuaaagacgc ccacgcgaag cggcagacgg uggucgugcu cggcucucag     1380

gagggggcug uucauaccgc ccuggccggc gcucuugaag ccgagaugga uggagcuaag     1440

ggcagacuga gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag     1500

ggagugagcu auucucucug uacagccgcg uucaccuuua caaaaauccc ggccgaaacc     1560

cugcauggca cggucaccgu ggagguucag uacgcuggga cagacggacc uugcaaggug     1620

cccgcccaaa uggcugucga uaugcagacc cugacaccug ugggcagacu cauuaccgcc     1680

aaccccguga ucacggaauc cacagagaac agcaagauga ugcuggagcu ugacccgccu     1740

uucggggauu cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau     1800

cgcuccggca gcacaauugg gaaagcguuu gaagccaccg ugcggggagc uaagagaaug     1860

gccgugcugg gcgacaccgc uugggauuuc ggguccgucg gaggcgcccu caauucucug     1920

gggaaaggca uccaccagau uuucggagcc gcuuuuaaga gccuguucgg cgggaugucc     1980

ugguuuucuc aaauccuuau cggaacacuc cugauguggc ugggccucaa cacgaagaau     2040

gggagcauuu cccugaugug ucuugcgcug ggaggcgugc ucaucuuccu gagcaccgcc     2100

guuucugcug acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8882
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8882
gggagaaagc uuaccaugaa ggagaaaaag agacggggcg ccgacaccag cguggggauc       60

gucggacugc uccugacaac ggcuauggcc gcggaaguua cccgcagagg cuccgcuuac      120

uauauguacc uugaucggaa cgacgccggg gaggccauuu cuuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcccgcaga      360

ucucggcgcg cuguuacccu ucccagccau uccacaagaa agcugcaaac ccggucucag      420

acauggcucg agagccgcga auacaccaag caccugauca gaguggagaa uuggauuuuu      480

cggaacccug gcuucgcccu ggcggccgcu gccaucgcuu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cuccauucgc      600

ugcaucggag uuagcaauag agauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggccc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaac auggcugaag uccggagcua cugcuaugag      780

gccucuauuu ccgauauggc uagcgacucc cgcuguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg gggcuggggc      900

aaugggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaccucg aauacagaau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaaugaua ccggacauga gacggacgag     1080

aaccgcgcga aaguugaaau cacacccaau ucuccccggg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu agaaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagcgc cagacggugg ucgugcucgg cucucaggag     1380

ggggcuguuc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cggcugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccggcucau uaccgccaac     1680

cccgugauca cggaauccac agagaacagc aagaugaugc uggagcuuga cccgccuuuc     1740

ggggauucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcauaga     1800

uccggcagca caauugggaa agcguuugaa gccaccgugc gcggagcuaa gcggauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccgucggag gcgcccucaa uucucugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugcugacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8883
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8883
gggagaaagc uuaccaugga gaagaaaaga cggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc uauggccgcg gaaguuaccc gcagaggcuc cgcuuacuau      120

auguaccuug aucggaacga cgccggggag gccauuucuu uccccacaac ccugggaaug      180

aauaagugcu auauccagau uauggaucuc ggccacaugu gugacgcuac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaacacc      300

acauccaccu gggucgugua uggaacgugu caucacaaaa agggcgaggc ccgcagaucu      360

cggcgcgcug uuacccuucc cagccauucc acaagaaaac ugcaaacccg gucucagaca      420

uggcucgaga gccgcgaaua caccaagcac cugaucagag uggagaauug gauuuuucgg      480

aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc uccugggguc cagcacgucu      540

cagaagguca ucuaucuugu gaugauucug cucaucgccc ccgcguacuc cauucgcugc      600

aucggaguua gcaauagaga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcccagg auaaaccuac aguugacauc      720

gagcuuguga ccaccacagu guccaacaug gcugaagucc ggagcuacug cuaugaggcc      780

ucuauuuccg auauggcuag cgacucccgc ugucccacgc aaggagaagc cuaccuggac      840

aagcagucug auacccagua ugugugcaaa agaacacucg uugaccgggg cuggggcaau      900

ggguguggac uguucggcaa ggggagccug gugaccugcg ccaaauuugc uugcuccaag      960

aagaugacag gaaaaagcau ccagccugag aaccucgaau acagaaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aaugauaccg gacaugagac ggacgagaac     1080

cgcgcgaagg uugaaaucac acccaauucu ccccgggccg aggcuacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccuaga accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga acaauaaaca cuggcucgug cauaaggagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggccga uaccggaaca ccccauugga acaauaaaga agcucugguc     1320

gaguuuaagg acgcccacgc gaaacgccag acgguggucg ugcucggcuc ucaggagggg     1380

gcuguucaua ccgcccuggc cggcgcucuu gaagccgaga uggauggagc uaagggccgg     1440

cugagcuccg ggcaccucaa gugcagacug aaaauggaca aacuucgccu gaagggagug     1500

agcuauucuc ucuguacagc cgcguucacc uuuacaaaga ucccggccga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcu gggacagacg gaccuugcaa agugcccgcc     1620

caaauggcug ucgauaugca gacccugaca ccugugggcc ggcucauuac cgccaacccc     1680

gugaucacgg aauccacaga gaacagcaag augaugcugg agcuugaccc gccuuucggg     1740

gauucuuaca ucguuauugg cgucggagaa aagaaaauca cccaccacug gcauagaucc     1800

ggcagcacaa uugggaaggc guuugaggcc accgugcgcg gagcuaaacg gauggccgug     1860

cugggcgaca ccgcuuggga uuucgggucc gucggaggcg cccucaauuc ucuggggaag     1920

ggcauccacc agauuuucgg agccgcuuuu aaaagccugu ucggcgggau guccugguuu     1980

ucucaaaucc uuaucggaac acuccugaug uggcugggcc ucaacacgaa gaaugggagc     2040

auuucccuga ugugucuugc gcugggaggc gugcucaucu uccugagcac cgccguuucu     2100

gcugacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8884
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8884
gggagaaagc uuaccaugaa gaaaagacgg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuga caacggcuau ggccgcggag guuacccgca gaggcuccgc uuacuauaug      120

uaccuugauc ggaacgacgc cggggaagcc auuucuuucc ccacaacccu gggaaugaau      180

aagugcuaua uccagauuau ggaucucggc cacaugugug acgcuacaau gagcuacgag      240

ugcccuaugc uggaugaagg gguggagccg gacgaugugg acuguuggug caacaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaaaaagg gcgaagcccg cagaucucgg      360

cgcgcuguua cccuucccag ccauuccaca agaaaacugc aaacccgguc ucagacaugg      420

cucgagagcc gcgaguacac caagcaccug aucagagugg aaaauuggau uuuucggaac      480

ccuggcuucg cccuggcggc cgcugccauc gcuuggcucc ugggguccag cacgucucag      540

aaggucaucu aucuugugau gauucugcuc aucgcccccg cguacuccau ucgcugcauc      600

ggaguuagca auagagauuu uguggagggc augucugggg gaacaugggu cgacauugug      660

cuggaacaug gcgggugugu gaccgucaug gcccaggaua aaccuacagu ugacaucgag      720

cuugugacca ccacaguguc caacauggcu gagguccgga gcuacugcua ugaagccucu      780

auuuccgaua uggcuagcga cucccgcugu cccacgcaag gagaggccua ccuggacaag      840

cagucugaua cccaguaugu gugcaaaaga acacucguug accggggcug gggcaauggg      900

uguggacugu ucggcaaggg gagccuggug accugcgcca aauuugcuug cuccaagaag      960

augacaggaa aaagcaucca gccugaaaac cucgaguaca gaaucaugcu gucuguccac     1020

ggcucccaac acagcgggau gauugugaau gauaccggac augaaacgga cgagaaccgc     1080

gcgaagguug agaucacacc caauucuccc cgggccgaag cuacccuugg cggcuucggg     1140

agccugggac ucgauuguga gccuagaacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaca auaaacacug gcucgugcau aaggaauggu uccacgacau uccccugccu     1260

uggcacgccg gggccgauac cggaacaccc cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggacg cccacgcgaa acgccagacg guggucgugc ucggcucuca ggagggggcu     1380

guucauaccg cccuggccgg cgcucuugag gccgaaaugg auggagcuaa gggccggcug     1440

agcuccgggc accucaagug cagacugaaa auggacaaac uucgccugaa gggagugagc     1500

uauucucucu guacagccgc guucaccuuu acaaagaucc cggccgagac ccugcauggc     1560

acggucaccg uggaaguuca guacgcuggg acagacggac cuugcaaagu gcccgcccaa     1620

auggcugucg auaugcagac ccugacaccu gugggccggc ucauuaccgc caaccccgug     1680

aucacggagu ccacagaaaa cagcaagaug augcuggagc uugacccgcc uuucggggau     1740

ucuuacaucg uuauuggcgu cggagagaag aaaaucaccc accacuggca uagauccggc     1800

agcacaauug ggaaggcguu ugaagccacc gugcgcggag cuaaacggau ggccgugcug     1860

ggcgacaccg cuugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaagggc     1920

auccaccaga uuuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu     2040

ucccugaugu gucuugcgcu gggaggcgug cucaucuucc ugagcaccgc cguuucugcu     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8885
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8885
gggagaaagc uuaccaugaa gagacggggc gccgacacca gcguggggau cgucggacug       60

cuccugacaa cggcuauggc cgcggagguu acccgcagag gcuccgcuua cuauauguac      120

cuugaucgga acgacgccgg ggaagccauu ucuuucccca caacccuggg aaugaauaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg cuacaaugag cuacgagugc      240

ccuaugcugg augaaggggu ggagccggac gauguggacu guuggugcaa caccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aagcccgcag aucucggcgc      360

gcuguuaccc uucccagcca uuccacaaga aagcugcaaa cccggucuca gacauggcuc      420

gagagccgcg aguacaccaa acaccugauc agaguggaaa auuggauuuu ucggaacccu      480

ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaag      540

gucaucuauc uugugaugau ucugcucauc gcccccgcgu acuccauucg cugcaucgga      600

guuagcaaua gagauuuugu ggagggcaug ucugggggaa caugggucga cauugugcug      660

gaacauggcg ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa cauggcugag guccggagcu acugcuauga agccucuauu      780

uccgauaugg cuagcgacuc ccgcuguccc acgcaaggag aggccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagagaaca cucguugacc ggggcugggg caaugggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caaaaagaug      960

acaggaaaga gcauccagcc ugaaaaccuc gaguacagaa ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaaugau accggacaug aaacggacga gaaccgcgcg     1080

aaaguugaga ucacacccaa uucuccccgg gccgaagcua cccuuggcgg cuucgggagc     1140

cugggacucg auugugagcc uagaaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaacaaua agcacuggcu cgugcauaaa gaaugguucc acgacauucc ccugccuugg     1260

cacgccgggg ccgauaccgg aacaccccau uggaacaaua aggaggcucu ggucgaauuu     1320

aaagacgccc acgcgaagcg ccagacggug gucgugcucg gcucucagga gggggcuguu     1380

cauaccgccc uggccggcgc ucuugaggcc gaaauggaug gagcuaaagg ccggcugagc     1440

uccgggcacc ucaagugcag acugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucucucugua cagccgcguu caccuuuaca aaaaucccgg ccgagacccu gcauggcacg     1560

gucaccgugg aaguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug     1620

gcugucgaua ugcagacccu gacaccugug ggccggcuca uuaccgccaa ccccgugauc     1680

acggagucca cagaaaacag caaaaugaug cuggagcuug acccgccuuu cggggauucu     1740

uacaucguua uuggcgucgg agagaagaag aucacccacc acuggcauag auccggcagc     1800

acaauuggga aagcguuuga agccaccgug cgcggagcua agcggauggc cgugcugggc     1860

gacaccgcuu gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaca cgaaaaaugg gagcauuucc     2040

cugauguguc uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcugac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8886
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8886
gggagaaagc uuaccaugag acggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg cuauggccgc ggagguuacc cgcagaggcu ccgcuuacua uauguaccuu      120

gaucggaacg acgccgggga agccauuucu uuccccacaa cccugggaau gaauaagugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcua caaugagcua cgagugcccu      240

augcuggaug aaggggugga gccggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaaa aagggcgaag cccgcagauc ucggcgcgcu      360

guuacccuuc ccagccauuc cacaagaaaa cugcaaaccc ggucucagac auggcucgag      420

agccgcgagu acaccaagca ccugaucaga guggaaaauu ggauuuuucg gaacccuggc      480

uucgcccugg cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu      600

agcaauagag auuuugugga gggcaugucu gggggaacau gggucgacau ugugcuggaa      660

cauggcgggu gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggcugagguc cggagcuacu gcuaugaagc cucuauuucc      780

gauauggcua gcgacucccg cugucccacg caaggagagg ccuaccugga caagcagucu      840

gauacccagu augugugcaa aagaacacuc guugaccggg gcuggggcaa ugggugugga      900

cuguucggca aggggagccu ggugaccugc gccaaauuug cuugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga aaaccucgag uacagaauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaaa cggacgagaa ccgcgcgaag     1080

guugagauca cacccaauuc uccccgggcc gaagcuaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugagccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccccu gccuuggcac     1260

gccggggccg auaccggaac accccauugg aacaauaaag aggcucuggu cgaauuuaag     1320

gacgcccacg cgaaacgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau     1380

accgcccugg ccggcgcucu ugaggccgaa auggauggag cuaagggccg gcugagcucc     1440

gggcaccuca aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu     1500

cucuguacag ccgcguucac cuuuacaaag aucccggccg agacccugca uggcacgguc     1560

accguggaag uucaguacgc ugggacagac ggaccuugca aagugcccgc ccaaauggcu     1620

gucgauaugc agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca     1800

auugggaagg cguuugaagc caccgugcgc ggagcuaaac ggauggccgu gcugggcgac     1860

accgcuuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa gggcauccac     1920

cagauuuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcugacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8887
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8887
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga gguuacccgg cgcggcuccg cuuacuauau guaccuugau      120

agaaacgacg ccggggaagc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga gugcccuaug      240

cuggaugaag ggguggagcc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaagccc ggcgcucuag acgggcuguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgagagc      420

cgggaguaca ccaagcaccu gauccgcgug gaaaauugga uuuuuagaaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaggg caugucuggg ggaacauggg ucgacauugu gcuggaacau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaggucaga agcuacugcu augaagccuc uauuuccgau      780

auggcuagcg acucccggug ucccacgcaa ggagaggccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugaaaa ccucgaguac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugaaacgg acgagaacag agcgaagguu     1080

gagaucacac ccaauucucc ccgcgccgaa gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug agccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggaaugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaagagg cucuggucga auuuaaggac     1320

gcccacgcga aaagacagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga ggccgaaaug gauggagcua agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaagauc ccggccgaga cccugcaugg cacggucacc     1560

guggaaguuc aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg ccaaccccgu gaucacggag     1680

uccacagaaa acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggcgu uugaagccac cgugagagga gcuaaacgca uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc ugacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8888
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8888
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaggu uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

aacgacgccg gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug      240

gaugaagggg uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caaaaagggc gaagccagac ggucucgcag agcuguuacc      360

cuucccagcc auuccacacg gaaacugcaa acccgcucuc agacauggcu cgagagcaga      420

gaguacacca agcaccugau ccggguggaa aauuggauuu uucgcaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauua gaugcaucgg aguuagcaau      600

cgggauuuug uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga gguccgcagc uacugcuaug aagccucuau uuccgauaug      780

gcuagcgacu ccagaugucc cacgcaagga gaggccuacc uggacaagca gucugauacc      840

caguaugugu gcaaacggac acucguugac cgcggcuggg gcaaugggug uggacuguuc      900

ggcaagggga gccuggugac cugcgccaaa uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gaaacggacg agaaccgggc gaagguugag     1080

aucacaccca auucucccag agccgaagcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugagc cucgcaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc     1320

cacgcgaaac ggcagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaggc cgaaauggau ggagcuaagg gcagacugag cuccgggcac     1440

cucaaaugcc gccugaagau ggacaaacuu cggcugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug     1560

gaaguucagu acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggcagacuc auuaccgcca accccgugau cacggagucc     1680

acagaaaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcauc gcuccggcag cacaauuggg     1800

aaggcguuug aagccaccgu gcggggagcu aaaagaaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu     1920

uucggagccg cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8889
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8889
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccugacaacg       60

gcuauggccg cggagguuac cagacgggga uccgcuuacu auauguaccu ugaucgcaac      120

gacgccggcg aagccauuuc uuuccccaca acccugggga ugaauaagug cuauauccag      180

auuauggauc ucggacacau gugugacgcu acaaugagcu acgagugccc uaugcuggau      240

gaaggcgugg agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uaugggacgu gucaucacaa aaagggagaa gccagacggu cucgcagagc uguuacccuu      360

cccagccauu ccacacggaa acugcaaacc cgcucucaga cauggcucga gagcagagag      420

uacaccaagc accugauccg gguggaaaau uggauuuuuc gcaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggc uccagcacgu cucagaaagu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauuagau gcaucggggu uagcaaucgg      600

gauuuugugg agggaauguc uggcgggaca ugggucgaca uugugcugga acauggaggc      660

ugugugaccg ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaggu ccgcagcuac ugcuaugaag ccucuauuuc cgauauggcu      780

agcgacucca gaugucccac gcaaggggag gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aacggacacu cguugaccgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucca agaaaaugac aggaaagagc      960

auccagccug aaaaccucga guacagaauc augcugucug uccacggguc ccaacacagc     1020

ggcaugauug ugaaugauac cgggcaugaa acggacgaga accgggcgaa gguugagauc     1080

acacccaauu cucccagagc cgaagcuacc cuuggaggcu ucggcagccu ggggcucgau     1140

ugugagccuc gcaccggacu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga augguuccac gacauucccc ugccuuggca cgccggcgcc     1260

gauaccggga caccccauug gaacaauaaa gaggcucugg ucgaauuuaa ggacgcccac     1320

gcgaaacggc agacgguggu cgugcucgga ucucaggagg gcgcuguuca uaccgcccug     1380

gccggggcuc uugaggccga aauggauggc gcuaagggaa gacugagcuc cggccaccuc     1440

aaaugccgcc ugaagaugga caagcugcgg cugaaagggg ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gagacccugc auggaacggu caccguggaa     1560

guucaguacg cuggcacaga cgggccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg aagacucauu accgccaacc ccgugaucac ggaguccaca     1680

gaaaacagca agaugaugcu ggagcuugac ccgccuuucg gcgauucuua caucguuauu     1740

ggggucggcg agaaaaagau cacccaccac uggcaucgcu ccggaagcac aauuggcaag     1800

gcguuugaag ccaccgugag aggggcuaaa cggauggccg ugcugggaga caccgcuugg     1860

gauuucggcu ccgucggggg agcccucaau ucucugggca aggggaucca ccagauuuuc     1920

ggcgccgcuu uuaaaagccu guucggaggc auguccuggu uuucucaaau ccuuaucggg     1980

acacuccuga uguggcuggg acucaacacg aagaauggca gcauuucccu gaugugucuu     2040

gcgcuugggg gagugcucau cuuccugagc accgccguuu cugcugacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8890
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8890
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu gacaacggcc       60

auggcugccg agguuaccag acggggaucc gcguacuaua uguaccuuga ucgcaacgac      120

gcuggcgaag ccauuucuuu ccccacaacc cuggggauga auaagugcua uauccagauu      180

auggaucucg gacacaugug ugacgccaca augagcuacg agugcccuau gcuggaugaa      240

ggcguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

gggacguguc aucacaaaaa gggagaagcu agacggucuc gcagagccgu uacccuuccc      360

agccauucca cacggaaacu gcaaacccgc ucucagacau ggcucgagag cagagaguac      420

accaagcacc ugauccgggu ggaaaauugg auuuuucgca acccuggcuu cgcucuggcc      480

gcggccgcua ucgccuggcu ccugggcucc agcacgucuc agaaagucau cuaucuugug      540

augauucugc ucaucgcucc cgccuacucc auuagaugca ucgggguuag caaucgggau      600

uuuguggagg gaaugucugg cgggacaugg gucgacauug ugcuggaaca uggaggcugu      660

gugaccguca uggcgcagga uaagccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg ccgagguccg cagcuacugc uaugaagcua gcauuuccga uauggccagc      780

gacuccagau gucccacgca aggggaggcu uaccuggaca agcagucuga uacccaguau      840

gugugcaaac ggacacucgu ugaccgcgga uggggcaaug gcugugggcu guucggaaag      900

ggcagccugg ugaccugcgc caaauuugcc ugcuccaaga aaaugacagg gaagucuauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggauccca acacagcggc     1020

augauuguga augauaccgg gcaugaaacg gacgagaacc gggcuaaggu ugagaucaca     1080

cccaauucuc ccagagcgga agccacccuu ggaggcuucg gcagccuggg gcucgauugu     1140

gagccucgca ccggacugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc uggcgccgau     1260

accgggacac cccauuggaa caauaaagag gcccuggucg aauuuaagga cgcucacgcc     1320

aaacggcaga cgguggucgu gcucggaucu caggagggcg cgguucauac cgcucuggcc     1380

ggggcccuug aggcugaaau ggauggcgcc aagggaagac ugagcuccgg ccaccucaaa     1440

ugccgccuga agauggacaa gcugcggcug aaagggguga gcuauucucu cuguacagcu     1500

gccuucaccu uuacaaagau cccggcggag acccugcaug gaacggucac cguggaaguu     1560

caguacgccg gcacagacgg gccuugcaaa gugcccgcuc aaauggccgu cgauaugcag     1620

acccugacac cugugggaag acucauuacc gcuaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucggcg auucuuacau cguuauuggg     1740

gucggcgaga aaaagaucac ccaccacugg caucgcuccg gaagcacaau uggcaaggcc     1800

uuugaagcga ccgugagagg ggccaaacgg auggcugugc ugggagacac cgccugggau     1860

uucggcuccg ucgggggagc ucucaauucu cugggcaagg ggauccacca gauuuucggc     1920

gccgccuuua aaagccuguu cggaggcaug uccugguuuu cucaaauccu uaucgggaca     1980

cuccugaugu ggcugggacu caacacgaag aauggcagca uuucccugau gugucuugcu     2040

cuugggggag ugcucaucuu ccugagcacc gcgguuucug ccgacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  8891
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8891
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccg auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8892
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8892
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgcgg uuaccagacg gggauccgcu uacuauaugu accuugaccg caacgaugcc      120

ggcgaggcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugcuacaaug agcuacgaau gcccuaugcu ggacgagggc      240

guggaaccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaggccaga cggucucgca gagcuguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgaaagcag agaguacacc      420

aagcaccuga uccgggugga gaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggaaggaa ugucuggcgg gacauggguc gauauugugc uggagcaugg aggcugugug      660

accgucaugg cccaggacaa gccuacaguu gauaucgaac uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaggccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaagccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauccag      960

ccugagaacc ucgaauacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugagacggau gaaaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaggc uacccuugga ggcuucggca gccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccccugccuu ggcacgccgg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaagcu cuggucgagu uuaaggaugc ccacgcgaaa     1320

cggcagacgg uggucgugcu cggaucucag gaaggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgagaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgaaacc cugcauggaa cggucaccgu ggagguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaggccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcug auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8893
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8893
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcggc uguuacccgg cgcggcuccg ccuacuauau guaccuugau      120

agaaacgacg ccggggaggc uauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgccacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaggcuc ggcgcucuag acgggccguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgaaagc      420

cgggaguaca ccaagcaccu gauccgcgug gagaauugga uuuuuagaaa cccuggcuuc      480

gcgcuggccg cugccgcuau cgccuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgcgccc gccuacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcucaggau aagccuacag uugacaucga acuugugacc      720

accacagugu ccaacauggc cgaggucaga agcuacugcu augaggcuuc uauuuccgau      780

auggccagcg acucccggug ucccacgcaa ggagaagccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcu aaauuugcgu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgaaaacag agccaagguu     1080

gagaucacac ccaauucucc ccgcgcugag gccacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggcugaua ccggaacacc ccauuggaac aauaaagaag cccuggucga guuuaaggac     1320

gcgcacgcua aaagacagac gguggucgug cucggcucuc aggaaggggc cguucauacc     1380

gcccuggcug gcgcccuuga ggcugagaug gauggagcca agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagcgg ccuucaccuu uacaaagauc ccggcugaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgccgg gacagacgga ccuugcaaag ugcccgcuca aauggccguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg cgaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggaa cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggccu uugaggcuac cgugagagga gccaaacgca uggcugugcu gggcgacacc     1860

gccugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag cugcguuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugccc ugggaggcgu gcucaucuuc cugagcaccg cuguuucugc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8894
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8894
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gaggcguuac gcggcgcggg      120

agcgccuacu auauguaccu ggauagaaac gacgcuggag aggccauuuc cuuucccacc      180

acacuuggca ugaauaaaug cuauauccaa auuauggauc uggggcauau gugugacgcg      240

accaugucuu acgaaugccc uaugcucgau gagggagugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggcaccu gucaccauaa gaaaggcgag      360

gcucggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg guuugcccuu gcugccgcug ccaucgcgug gcugcucgga      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ccccgcuuac      600

ucuauucggu gcaucggcgu uuccaaucgc gauuucgugg aagggaugag cggaggcacg      660

ugggucgaca uugugcucga gcacggggga ugugugacag ucauggccca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggcugaggu cagauccuac      780

ugcuaugagg ccagcauuuc cgauauggcg ucugacagcc ggugucccac acagggcgaa      840

gccuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggga auggaugugg ccuguuuggg aaaggaagcc uugugacaug cgcuaaguuc      960

gccugcucua aaaagaugac cggcaagucc auccaaccug agaaccugga auaccggauc     1020

augcucagcg uccacggguc ucagcauagc ggaaugauug ugaaugauac aggccacgag     1080

accgacgaaa acagagcuaa aguugagauc acgcccaauu ccccgcgcgc cgaggccaca     1140

cugggcgggu uuggaucucu uggccuggau ugugaaccuc ggaccgggcu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcuggagcg gauacaggca caccccacug gaacaauaag     1320

gaagcccuug ucgaguucaa agacgcucau gccaagagac agacaguggu cgugcugggg     1380

ucccaggaag gcgccguuca cacggcucuc gccggagcgc uggaggcuga gauggauggc     1440

gccaaagggc gccuuagcuc uggacaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggcg uguccuauag ccucuguacc gccgcuuuua cauucaccaa aaucccggcc     1560

gaaacacugc acgggaccgu cacgguggag guucaauacg cuggaaccga cggcccuugc     1620

aaggugcccg cccagauggc ggucgauaug cagacacuua ccccuguggg gcgccugauu     1680

acagccaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gcgacagcua caucguuauu ggagucggcg agaagaagau cacacaccau     1800

uggcaccggu ccgggucuac cauuggaaag gcuuucgagg ccacagugag aggcgcuaaa     1860

cgcauggccg ugcuggggga caccgcgugg gauuucggaa gcgucggcgg ggcccuuaau     1920

ucccucggca aaggaaucca ucaaauuuuu ggcgcugccu ucaagucucu guuuggggga     1980

augagcuggu ucucccagau ccugaucggc acccuccuga uguggcuugg gcugaacacc     2040

aaaaauggaa gcauuucucu caugugucug gcucugggcg gggugcuuau cuuucucucc     2100

acggccguua gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8895
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8895
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gagccguuac gcggcgcggc      120

agcgcuuacu auauguaccu ggauagaaac gacgccgggg aggcgauuuc cuuucccacc      180

acacuuggaa ugaauaaaug cuauauccaa auuauggauc ugggccauau gugugacgcu      240

accaugucuu acgaaugccc uaugcucgau gagggggugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggaaccu gucaccauaa gaaaggcgag      360

gcccggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg cuuugcucuu gccgcugccg cgaucgccug gcugcucggg      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ucccgccuac      600

ucuauucggu gcaucggagu uuccaaucgc gauuucgugg aaggcaugag cgggggaacg      660

ugggucgaca uugugcucga gcacggcggg ugugugacag ucauggcuca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggccgaggu cagauccuac      780

ugcuaugagg cgagcauuuc cgauauggcc ucugacagcc ggugucccac acagggagag      840

gcuuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggca augggugugg acuguuuggc aaagggagcc uugugacaug cgccaaguuc      960

gcuugcucua aaaagaugac cggaaagucc auccaaccug aaaaccugga auaccggauc     1020

augcucagcg uccacggcuc ucagcauagc gggaugauug ugaaugauac aggacacgag     1080

accgacgaaa acagagccaa aguugagauc acgcccaauu ccccgcgcgc cgaggcuaca     1140

cugggcggcu uugggucucu uggacuggau ugugaaccuc ggaccggccu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcgggggcc gauacaggaa caccccacug gaacaauaag     1320

gaagcucuug ucgaguucaa agacgcccau gccaagagac agacaguggu cgugcugggc     1380

ucccaggaag gggcuguuca cacggcccuc gcgggcgcuc uggaggccga gauggaugga     1440

gccaaaggcc gccuuagcuc ugggcaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggag uguccuauag ccucuguacc gcugccuuua cauucaccaa aaucccggcu     1560

gaaacacugc acggcaccgu cacgguggag guucaauacg ccgggaccga cggaccuugc     1620

aaggugcccg cgcagauggc cgucgauaug cagacacuua ccccuguggg ccgccugauu     1680

acagcuaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gggacagcua caucguuauu ggcgucggag agaagaagau cacacaccau     1800

uggcaccggu ccggcucuac cauugggaaa gccuucgagg cuacagugag aggagccaag     1860

cgcauggcgg ugcugggcga caccgccugg gauuucggga gcgucggagg cgcucuuaau     1920

ucccucggga aaggcaucca ucaaauuuuu ggagccgcuu ucaagucucu guuuggcggg     1980

augagcuggu ucucccagau ccugaucgga acccuccuga uguggcuugg ccugaacacc     2040

aaaaauggga gcauuucucu caugugucug gcccugggag gcgugcuuau cuuucucucc     2100

acggccguua gcgcugacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8896
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8896
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  8897
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8897
gggagaaagc uuaccaugau caacgccaga aaggagaaaa agcggcgcgg cgcugacacc       60

agcgugggga uugucggacu gcuccugaca acggccaugg cggcugaagu uaccagacgg      120

ggcuccgccu acuauaugua ccuugaucgc aaugacgccg gggaggcuau cucuuucccc      180

acaacccugg gaaugaacaa augcuauauu cagaucaugg aucucggcca caugugugac      240

gccacaauga gcuacgaaug cccuaugcug gaugaggggg uggaaccgga cgauguggac      300

uguuggugca auaccacauc caccuggguc guguauggaa cgugucauca caagaaaggc      360

gaggcuagac ggucucgcag agccguuacc cuucccagcc auuccacacg gaagcugcaa      420

acccgcucuc agacauggcu cgagagcaga gaauacacca agcaccugau ucggguggag      480

aacuggaucu uucgcaaucc uggcuucgcg cuggccgcug ccgcuaucgc cuggcuccug      540

ggguccagca cgucucagaa agucauuuau cuugugauga uccugcucau ugcgcccgcc      600

uacuccauca gaugcauugg aguuagcaac cgggauuuug uggaaggcau gucuggggga      660

acaugggucg acaucgugcu ggagcauggc ggguguguga ccgucauggc ucaggauaag      720

ccuacaguug acauugagcu ugugaccacc acagugucca auauggccga aguccgcagc      780

uacugcuaug aggcuucuau cuccgauaug gccagcgacu ccagaugucc cacgcaagga      840

gaagccuacc uggacaaaca gucugauacc caguaugugu gcaagcggac acucguugac      900

agaggcuggg gcaacgggug uggacuguuc ggcaaaggga gccuggugac cugcgcuaag      960

uuugcgugcu ccaagaaaau gacaggaaag agcauccagc cugagaaucu cgaauaccgc     1020

auuaugcugu cuguccacgg cucccaacac agcgggauga ucgugaacga uaccggacau     1080

gagacggacg agaaucgggc caaaguugaa auuacaccga acucucccag agcugaggcc     1140

acccuuggcg gcuucgggag ccugggacuc gauugugaac cucgcaccgg ccuggacuuu     1200

uccgaucuuu auuaccugac aaugaauaac aagcacuggc ucgugcauaa agagugguuc     1260

cacgacaucc cccugccuug gcacgccggg gcugauaccg gaacacccca uuggaauaac     1320

aaggaagccc uggucgaguu uaaagacgcg cacgcuaagc ggcagacggu ggucgugcuc     1380

ggcucucagg agggggccgu ucauaccgcc cuggcuggcg cccuugaagc ugagauggau     1440

ggagccaagg gcagacugag cuccgggcac cucaaaugcc gccugaagau ggacaaacuu     1500

cggcugaagg gagugagcua uucucucugu acagcggccu ucaccuuuac aaaaauuccg     1560

gcugaaaccc ugcauggcac ggucaccgug gagguucagu acgccgggac agacggaccu     1620

ugcaaggugc ccgcucaaau ggccgucgau augcagaccc ugacaccugu gggcagacuc     1680

aucaccgcga accccgugau cacggaaucc acagagaaua gcaagaugau gcuggagcuu     1740

gaucccccuu ucggggacuc uuacauuguu aucggcgucg gagagaaaaa gauuacccac     1800

cacuggcauc gcuccggcag cacaaucggg aaagccuuug aagcuaccgu gcggggagcc     1860

aagagaaugg cugugcuggg cgacaccgcc ugggauuucg gguccgucgg aggcgcccuc     1920

aacucucugg ggaaaggcau ucaccagauc uucggagcug cguuuaagag ccuguucggc     1980

gggauguccu gguuuucuca aauccuuauu ggaacacucc ugauguggcu gggccucaau     2040

acgaagaacg ggagcaucuc ccugaugugu cuugcccugg gaggcgugcu cauuuuccug     2100

agcaccgcug uuucugccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  8898
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8898
gggagaaagc uuaccaugau cauuaacgcc agaaaggaga aaaagcggcg cggcgcugac       60

accagcgugg ggaucgucgg acugcuccug acaacggcca uggcggcuga aguuaccaga      120

cggggcuccg ccuacuauau guaccuugau cgcaaugacg ccggggaggc uauuucuuuc      180

cccacaaccc ugggaaugaa caaaugcuau auccagauua uggaucucgg ccacaugugu      240

gacgccacaa ugagcuacga augcccuaug cuggaugagg ggguggaacc ggacgaugug      300

gacuguuggu gcaauaccac auccaccugg gucguguaug gaacguguca ucacaagaaa      360

ggcgaggcua gacggucucg cagagccguu acccuuccca gccauuccac acggaagcug      420

caaacccgcu cucagacaug gcucgagagc agagaauaca ccaagcaccu gauccgggug      480

gagaacugga ucuuucgcaa uccuggcuuc gcgcuggccg cugccgcuau ugccuggcuc      540

cuggggucca gcacgucuca gaaagucauc uaucuuguga ugauucugcu caucgcgccc      600

gccuacucca uuagaugcau cggaguuagc aaccgggauu uuguggaagg caugucuggg      660

ggaacauggg ucgacauugu gcuggagcau ggcgggugug ugaccgucau ggcucaggau      720

aagccuacag uugacaucga gcuugugacc accacagugu ccaauauggc cgaaguccgc      780

agcuacugcu augaggcuuc uaucuccgau auggccagcg acuccagaug ucccacgcaa      840

ggagaagccu accuggacaa acagucugau acccaguaug ugugcaagcg gacacucguu      900

gacagaggcu ggggcaacgg guguggacug uucggcaaag ggagccuggu gaccugcgcu      960

aaguuugcgu gcuccaagaa aaugacagga aagagcauuc agccugagaa ucucgaauac     1020

cgcaucaugc ugucugucca cggcucccaa cacagcggga ugauugugaa cgauaccgga     1080

caugagacgg acgagaaucg ggccaaaguu gaaaucacac cgaacucucc cagagcugag     1140

gccacccuug gcggcuucgg gagccuggga cucgauugug aaccucgcac cggccuggac     1200

uuuuccgauc uuuauuaccu gacaaugaau aacaagcacu ggcucgugca uaaagagugg     1260

uuccacgaca uuccccugcc uuggcacgcc ggggcugaua ccggaacacc ccauuggaau     1320

aacaaggaag cccuggucga guuuaaagac gcgcacgcua agcggcagac gguggucgug     1380

cucggcucuc aggagggggc cguucauacc gcccuggcug gcgcccuuga agcugagaug     1440

gauggagcca agggcagacu gagcuccggg caccucaaau gccgccugaa gauggacaaa     1500

cuucggcuga agggagugag cuauucucuc uguacagcgg ccuucaccuu uacaaaaauc     1560

ccggcugaaa cccugcaugg cacggucacc guggagguuc aguacgccgg gacagacgga     1620

ccuugcaagg ugcccgcuca aauggccguc gauaugcaga cccugacacc ugugggcaga     1680

cucaucaccg cgaaccccgu gauuacggaa uccacagaga auagcaagau gaugcuggag     1740

cuugaucccc cuuucgggga cucuuacauc guuauuggcg ucggagagaa aaagaucacc     1800

caccacuggc aucgcuccgg cagcacaauu gggaaagccu uugaagcuac cgugcgggga     1860

gccaagagaa uggcugugcu gggcgacacc gccugggauu ucggguccgu cggaggcgcc     1920

cucaacucuc uggggaaagg cauccaccag aucuucggag cugcguuuaa gagccuguuc     1980

ggcgggaugu ccugguuuuc ucaaauucuu aucggaacac uccugaugug gcugggccuc     2040

aauacgaaga acgggagcau uucccugaug ugucuugccc ugggaggcgu gcucaucuuc     2100

cugagcaccg cuguuucugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  8899
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8899
gggagaaagc uuaccaugaa cgccagaaag gagaaaaagc ggcgcggcgc ugacaccagc       60

guggggaucg ucggacugcu ccugacaacg gccauggcgg cugaaguuac cagacggggc      120

uccgccuacu auauguaccu ugaucgcaau gacgccgggg aggcuauuuc uuuccccaca      180

acccugggaa ugaacaaaug cuauauccag auuauggauc ucggccacau gugugacgcc      240

acaaugagcu acgaaugccc uaugcuggau gagggggugg aaccggacga uguggacugu      300

uggugcaaua ccacauccac cugggucgug uauggaacgu gucaucacaa gaaaggcgag      360

gcuagacggu cucgcagagc cguuacccuu cccagccauu ccacacggaa gcugcaaacc      420

cgcucucaga cauggcucga gagcagagaa uacaccaagc accugauccg gguggagaac      480

uggauuuuuc gcaauccugg cuucgcgcug gccgcugccg cuaucgccug gcuccugggg      540

uccagcacgu cucagaaagu caucuaucuu gugaugauuc ugcucaucgc gcccgccuac      600

uccauuagau gcaucggagu uagcaaccgg gauuuugugg aaggcauguc ugggggaaca      660

ugggucgaca uugugcugga gcauggcggg ugugugaccg ucauggcuca ggauaagccu      720

acaguugaca ucgagcuugu gaccaccaca guguccaaua uggccgaagu ccgcagcuac      780

ugcuaugagg cuucuauuuc cgauauggcc agcgacucca gaugucccac gcaaggagaa      840

gccuaccugg acaaacaguc ugauacccag uaugugugca agcggacacu cguugacaga      900

ggcuggggca acgggugugg acuguucggc aaagggagcc uggugaccug cgcuaaguuu      960

gcgugcucca agaaaaugac aggaaagagc auccagccug agaaucucga auaccgcauc     1020

augcugucug uccacggcuc ccaacacagc gggaugauug ugaacgauac cggacaugag     1080

acggacgaga aucgggccaa aguugaaauc acaccgaacu cucccagagc ugaggccacc     1140

cuuggcggcu ucgggagccu gggacucgau ugugaaccuc gcaccggccu ggacuuuucc     1200

gaucuuuauu accugacaau gaauaacaag cacuggcucg ugcauaaaga gugguuccac     1260

gacauucccc ugccuuggca cgccggggcu gauaccggaa caccccauug gaauaacaag     1320

gaagcccugg ucgaguuuaa agacgcgcac gcuaagcggc agacgguggu cgugcucggc     1380

ucucaggagg gggccguuca uaccgcccug gcuggcgccc uugaagcuga gauggaugga     1440

gccaagggca gacugagcuc cgggcaccuc aaaugccgcc ugaagaugga caaacuucgg     1500

cugaagggag ugagcuauuc ucucuguaca gcggccuuca ccuuuacaaa aaucccggcu     1560

gaaacccugc auggcacggu caccguggag guucaguacg ccgggacaga cggaccuugc     1620

aaggugcccg cucaaauggc cgucgauaug cagacccuga caccuguggg cagacucauu     1680

accgcgaacc ccgugaucac ggaauccaca gagaauagca agaugaugcu ggagcuugau     1740

cccccuuucg gggacucuua caucguuauu ggcgucggag agaaaaagau cacccaccac     1800

uggcaucgcu ccggcagcac aauugggaaa gccuuugaag cuaccgugcg gggagccaag     1860

agaauggcug ugcugggcga caccgccugg gauuucgggu ccgucggagg cgcccucaac     1920

ucucugggga aaggcaucca ccagauuuuc ggagcugcgu uuaagagccu guucggcggg     1980

auguccuggu uuucucaaau ccuuaucgga acacuccuga uguggcuggg ccucaauacg     2040

aagaacggga gcauuucccu gaugugucuu gcccugggag gcgugcucau cuuccugagc     2100

accgcuguuu cugccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8900
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8900
gggagaaagc uuaccauggc cagaaaggag aaaaagcggc gcggcgcuga caccagcgug       60

gggaucgucg gacugcuccu gacaacggcc auggcggcug aaguuaccag acggggcucc      120

gccuacuaua uguaccuuga ucgcaacgac gccggggagg cuauuucuuu ccccacaacc      180

cugggaauga auaaaugcua uauccagauu auggaucucg gccacaugug ugacgccaca      240

augagcuacg aaugcccuau gcuggaugag gggguggaac cggacgaugu ggacuguugg      300

ugcaacacca cauccaccug ggucguguau ggaacguguc aucacaagaa aggcgaggcu      360

agacggucuc gcagagccgu uacccuuccc agccauucca cacggaagcu gcaaacccgc      420

ucucagacau ggcucgagag cagagaauac accaagcacc ugauccgggu ggagaauugg      480

auuuuucgca acccuggcuu cgcgcuggcc gcugccgcua ucgccuggcu ccuggggucc      540

agcacgucuc agaaagucau cuaucuugug augauucugc ucaucgcgcc cgccuacucc      600

auuagaugca ucggaguuag caaucgggau uuuguggaag gcaugucugg gggaacaugg      660

gucgacauug ugcuggagca uggcgggugu gugaccguca uggcucagga uaagccuaca      720

guugacaucg agcuugugac caccacagug uccaacaugg ccgaaguccg cagcuacugc      780

uaugaggcuu cuauuuccga uauggccagc gacuccagau gucccacgca aggagaagcc      840

uaccuggaca aacagucuga uacccaguau gugugcaagc ggacacucgu ugacagaggc      900

uggggcaaug gguguggacu guucggcaaa gggagccugg ugaccugcgc uaaguuugcg      960

ugcuccaaga aaaugacagg aaagagcauc cagccugaga accucgaaua ccgcaucaug     1020

cugucugucc acggcuccca acacagcggg augauuguga augauaccgg acaugagacg     1080

gacgagaacc gggccaaagu ugaaaucaca cccaauucuc ccagagcuga ggccacccuu     1140

ggcggcuucg ggagccuggg acucgauugu gaaccucgca ccggccugga cuuuuccgau     1200

cuuuauuacc ugacaaugaa caauaagcac uggcucgugc auaaagagug guuccacgac     1260

auuccccugc cuuggcacgc cggggcugau accggaacac cccauuggaa caauaaggaa     1320

gcccuggucg aguuuaaaga cgcgcacgcu aagcggcaga cgguggucgu gcucggcucu     1380

caggaggggg ccguucauac cgcccuggcu ggcgcccuug aagcugagau ggauggagcc     1440

aagggcagac ugagcuccgg gcaccucaaa ugccgccuga agauggacaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcg gccuucaccu uuacaaaaau cccggcugaa     1560

acccugcaug gcacggucac cguggagguu caguacgccg ggacagacgg accuugcaag     1620

gugcccgcuc aaauggccgu cgauaugcag acccugacac cugugggcag acucauuacc     1680

gcgaaccccg ugaucacgga auccacagag aacagcaaga ugaugcugga gcuugacccg     1740

ccuuucgggg auucuuacau cguuauuggc gucggagaga aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaagcc uuugaagcua ccgugcgggg agccaagaga     1860

auggcugugc ugggcgacac cgccugggau uucggguccg ucggaggcgc ccucaauucu     1920

cuggggaaag gcauccacca gauuuucgga gcugcguuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauccu uaucggaaca cuccugaugu ggcugggccu caacacgaag     2040

aaugggagca uuucccugau gugucuugcc cugggaggcg ugcucaucuu ccugagcacc     2100

gcuguuucug ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  8901
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8901
gggagaaagc uuaccaugag aaaggagaaa aagcggcgcg gcgccgacac cagcgugggg       60

aucgucggac ugcuccugac aacggcuaug gccgcggaag uuaccagacg gggcuccgcu      120

uacuauaugu accuugaucg caacgacgcc ggggaggcca uuucuuuccc cacaacccug      180

ggaaugaaua aaugcuauau ccagauuaug gaucucggcc acauguguga cgcuacaaug      240

agcuacgaau gcccuaugcu ggaugagggg guggaaccgg acgaugugga cuguuggugc      300

aacaccacau ccaccugggu cguguaugga acgugucauc acaagaaagg cgaggccaga      360

cggucucgca gagcuguuac ccuucccagc cauuccacac ggaagcugca aacccgcucu      420

cagacauggc ucgagagcag agaauacacc aagcaccuga uccgggugga gaauuggauu      480

uuucgcaacc cuggcuucgc ccuggcggcc gcugccaucg cuuggcuccu gggguccagc      540

acgucucaga aagucaucua ucuugugaug auucugcuca ucgcccccgc guacuccauu      600

agaugcaucg gaguuagcaa ucgggauuuu guggaaggca ugucuggggg aacauggguc      660

gacauugugc uggagcaugg cgggugugug accgucaugg cccaggauaa gccuacaguu      720

gacaucgagc uugugaccac cacagugucc aacauggcug aaguccgcag cuacugcuau      780

gaggccucua uuuccgauau ggcuagcgac uccagauguc ccacgcaagg agaagccuac      840

cuggacaaac agucugauac ccaguaugug ugcaagcgga cacucguuga cagaggcugg      900

ggcaaugggu guggacuguu cggcaaaggg agccugguga ccugcgccaa guuugcuugc      960

uccaagaaaa ugacaggaaa gagcauccag ccugagaacc ucgaauaccg caucaugcug     1020

ucuguccacg gcucccaaca cagcgggaug auugugaaug auaccggaca ugagacggac     1080

gagaaccggg cgaaaguuga aaucacaccc aauucuccca gagccgaggc uacccuuggc     1140

ggcuucggga gccugggacu cgauugugaa ccucgcaccg gccuggacuu uuccgaucuu     1200

uauuaccuga caaugaacaa uaagcacugg cucgugcaua aagagugguu ccacgacauu     1260

ccccugccuu ggcacgccgg ggccgauacc ggaacacccc auuggaacaa uaaggaagcu     1320

cuggucgagu uuaaagacgc ccacgcgaag cggcagacgg uggucgugcu cggcucucag     1380

gagggggcug uucauaccgc ccuggccggc gcucuugaag ccgagaugga uggagcuaag     1440

ggcagacuga gcuccgggca ccucaaaugc cgccugaaga uggacaaacu ucggcugaag     1500

ggagugagcu auucucucug uacagccgcg uucaccuuua caaaaauccc ggccgaaacc     1560

cugcauggca cggucaccgu ggagguucag uacgcuggga cagacggacc uugcaaggug     1620

cccgcccaaa uggcugucga uaugcagacc cugacaccug ugggcagacu cauuaccgcc     1680

aaccccguga ucacggaauc cacagagaac agcaagauga ugcuggagcu ugacccgccu     1740

uucggggauu cuuacaucgu uauuggcguc ggagagaaaa agaucaccca ccacuggcau     1800

cgcuccggca gcacaauugg gaaagcguuu gaagccaccg ugcggggagc uaagagaaug     1860

gccgugcugg gcgacaccgc uugggauuuc ggguccgucg gaggcgcccu caauucucug     1920

gggaaaggca uccaccagau uuucggagcc gcuuuuaaga gccuguucgg cgggaugucc     1980

ugguuuucuc aaauccuuau cggaacacuc cugauguggc ugggccucaa cacgaagaau     2040

gggagcauuu cccugaugug ucuugcgcug ggaggcgugc ucaucuuccu gagcaccgcc     2100

guuucugcuu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  8902
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8902
gggagaaagc uuaccaugaa ggagaaaaag agacggggcg ccgacaccag cguggggauc       60

gucggacugc uccugacaac ggcuauggcc gcggaaguua cccgcagagg cuccgcuuac      120

uauauguacc uugaucggaa cgacgccggg gaggccauuu cuuuccccac aacccuggga      180

augaauaaau gcuauaucca gauuauggau cucggccaca ugugugacgc uacaaugagc      240

uacgaaugcc cuaugcugga ugagggggug gaaccggacg auguggacug uuggugcaac      300

accacaucca ccugggucgu guauggaacg ugucaucaca agaaaggcga ggcccgcaga      360

ucucggcgcg cuguuacccu ucccagccau uccacaagaa agcugcaaac ccggucucag      420

acauggcucg agagccgcga auacaccaag caccugauca gaguggagaa uuggauuuuu      480

cggaacccug gcuucgcccu ggcggccgcu gccaucgcuu ggcuccuggg guccagcacg      540

ucucagaaag ucaucuaucu ugugaugauu cugcucaucg cccccgcgua cuccauucgc      600

ugcaucggag uuagcaauag agauuuugug gaaggcaugu cugggggaac augggucgac      660

auugugcugg agcauggcgg gugugugacc gucauggccc aggauaagcc uacaguugac      720

aucgagcuug ugaccaccac aguguccaac auggcugaag uccggagcua cugcuaugag      780

gccucuauuu ccgauauggc uagcgacucc cgcuguccca cgcaaggaga agccuaccug      840

gacaaacagu cugauaccca guaugugugc aagagaacac ucguugaccg gggcuggggc      900

aaugggugug gacuguucgg caaagggagc cuggugaccu gcgccaaguu ugcuugcucc      960

aagaaaauga caggaaagag cauccagccu gagaaccucg aauacagaau caugcugucu     1020

guccacggcu cccaacacag cgggaugauu gugaaugaua ccggacauga gacggacgag     1080

aaccgcgcga aaguugaaau cacacccaau ucuccccggg ccgaggcuac ccuuggcggc     1140

uucgggagcc ugggacucga uugugaaccu agaaccggcc uggacuuuuc cgaucuuuau     1200

uaccugacaa ugaacaauaa gcacuggcuc gugcauaaag agugguucca cgacauuccc     1260

cugccuuggc acgccggggc cgauaccgga acaccccauu ggaacaauaa ggaagcucug     1320

gucgaguuua aagacgccca cgcgaagcgc cagacggugg ucgugcucgg cucucaggag     1380

ggggcuguuc auaccgcccu ggccggcgcu cuugaagccg agauggaugg agcuaagggc     1440

cggcugagcu ccgggcaccu caaaugcaga cugaagaugg acaaacuucg ccugaaggga     1500

gugagcuauu cucucuguac agccgcguuc accuuuacaa aaaucccggc cgaaacccug     1560

cauggcacgg ucaccgugga gguucaguac gcugggacag acggaccuug caaggugccc     1620

gcccaaaugg cugucgauau gcagacccug acaccugugg gccggcucau uaccgccaac     1680

cccgugauca cggaauccac agagaacagc aagaugaugc uggagcuuga cccgccuuuc     1740

ggggauucuu acaucguuau uggcgucgga gagaaaaaga ucacccacca cuggcauaga     1800

uccggcagca caauugggaa agcguuugaa gccaccgugc gcggagcuaa gcggauggcc     1860

gugcugggcg acaccgcuug ggauuucggg uccgucggag gcgcccucaa uucucugggg     1920

aaaggcaucc accagauuuu cggagccgcu uuuaagagcc uguucggcgg gauguccugg     1980

uuuucucaaa uccuuaucgg aacacuccug auguggcugg gccucaacac gaagaauggg     2040

agcauuuccc ugaugugucu ugcgcuggga ggcgugcuca ucuuccugag caccgccguu     2100

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  8903
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8903
gggagaaagc uuaccaugga gaagaaaaga cggggcgccg acaccagcgu ggggaucguc       60

ggacugcucc ugacaacggc uauggccgcg gaaguuaccc gcagaggcuc cgcuuacuau      120

auguaccuug aucggaacga cgccggggag gccauuucuu uccccacaac ccugggaaug      180

aauaagugcu auauccagau uauggaucuc ggccacaugu gugacgcuac aaugagcuac      240

gaaugcccua ugcuggauga ggggguggaa ccggacgaug uggacuguug gugcaacacc      300

acauccaccu gggucgugua uggaacgugu caucacaaaa agggcgaggc ccgcagaucu      360

cggcgcgcug uuacccuucc cagccauucc acaagaaaac ugcaaacccg gucucagaca      420

uggcucgaga gccgcgaaua caccaagcac cugaucagag uggagaauug gauuuuucgg      480

aacccuggcu ucgcccuggc ggccgcugcc aucgcuuggc uccugggguc cagcacgucu      540

cagaagguca ucuaucuugu gaugauucug cucaucgccc ccgcguacuc cauucgcugc      600

aucggaguua gcaauagaga uuuuguggaa ggcaugucug ggggaacaug ggucgacauu      660

gugcuggagc auggcgggug ugugaccguc auggcccagg auaaaccuac aguugacauc      720

gagcuuguga ccaccacagu guccaacaug gcugaagucc ggagcuacug cuaugaggcc      780

ucuauuuccg auauggcuag cgacucccgc ugucccacgc aaggagaagc cuaccuggac      840

aagcagucug auacccagua ugugugcaaa agaacacucg uugaccgggg cuggggcaau      900

ggguguggac uguucggcaa ggggagccug gugaccugcg ccaaauuugc uugcuccaag      960

aagaugacag gaaaaagcau ccagccugag aaccucgaau acagaaucau gcugucuguc     1020

cacggcuccc aacacagcgg gaugauugug aaugauaccg gacaugagac ggacgagaac     1080

cgcgcgaagg uugaaaucac acccaauucu ccccgggccg aggcuacccu uggcggcuuc     1140

gggagccugg gacucgauug ugaaccuaga accggccugg acuuuuccga ucuuuauuac     1200

cugacaauga acaauaaaca cuggcucgug cauaaggagu gguuccacga cauuccccug     1260

ccuuggcacg ccggggccga uaccggaaca ccccauugga acaauaaaga agcucugguc     1320

gaguuuaagg acgcccacgc gaaacgccag acgguggucg ugcucggcuc ucaggagggg     1380

gcuguucaua ccgcccuggc cggcgcucuu gaagccgaga uggauggagc uaagggccgg     1440

cugagcuccg ggcaccucaa gugcagacug aaaauggaca aacuucgccu gaagggagug     1500

agcuauucuc ucuguacagc cgcguucacc uuuacaaaga ucccggccga aacccugcau     1560

ggcacgguca ccguggaggu ucaguacgcu gggacagacg gaccuugcaa agugcccgcc     1620

caaauggcug ucgauaugca gacccugaca ccugugggcc ggcucauuac cgccaacccc     1680

gugaucacgg aauccacaga gaacagcaag augaugcugg agcuugaccc gccuuucggg     1740

gauucuuaca ucguuauugg cgucggagaa aagaaaauca cccaccacug gcauagaucc     1800

ggcagcacaa uugggaaggc guuugaggcc accgugcgcg gagcuaaacg gauggccgug     1860

cugggcgaca ccgcuuggga uuucgggucc gucggaggcg cccucaauuc ucuggggaag     1920

ggcauccacc agauuuucgg agccgcuuuu aaaagccugu ucggcgggau guccugguuu     1980

ucucaaaucc uuaucggaac acuccugaug uggcugggcc ucaacacgaa gaaugggagc     2040

auuucccuga ugugucuugc gcugggaggc gugcucaucu uccugagcac cgccguuucu     2100

gcuugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  8904
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8904
gggagaaagc uuaccaugaa gaaaagacgg ggcgccgaca ccagcguggg gaucgucgga       60

cugcuccuga caacggcuau ggccgcggag guuacccgca gaggcuccgc uuacuauaug      120

uaccuugauc ggaacgacgc cggggaagcc auuucuuucc ccacaacccu gggaaugaau      180

aagugcuaua uccagauuau ggaucucggc cacaugugug acgcuacaau gagcuacgag      240

ugcccuaugc uggaugaagg gguggagccg gacgaugugg acuguuggug caacaccaca      300

uccaccuggg ucguguaugg aacgugucau cacaaaaagg gcgaagcccg cagaucucgg      360

cgcgcuguua cccuucccag ccauuccaca agaaaacugc aaacccgguc ucagacaugg      420

cucgagagcc gcgaguacac caagcaccug aucagagugg aaaauuggau uuuucggaac      480

ccuggcuucg cccuggcggc cgcugccauc gcuuggcucc ugggguccag cacgucucag      540

aaggucaucu aucuugugau gauucugcuc aucgcccccg cguacuccau ucgcugcauc      600

ggaguuagca auagagauuu uguggagggc augucugggg gaacaugggu cgacauugug      660

cuggaacaug gcgggugugu gaccgucaug gcccaggaua aaccuacagu ugacaucgag      720

cuugugacca ccacaguguc caacauggcu gagguccgga gcuacugcua ugaagccucu      780

auuuccgaua uggcuagcga cucccgcugu cccacgcaag gagaggccua ccuggacaag      840

cagucugaua cccaguaugu gugcaaaaga acacucguug accggggcug gggcaauggg      900

uguggacugu ucggcaaggg gagccuggug accugcgcca aauuugcuug cuccaagaag      960

augacaggaa aaagcaucca gccugaaaac cucgaguaca gaaucaugcu gucuguccac     1020

ggcucccaac acagcgggau gauugugaau gauaccggac augaaacgga cgagaaccgc     1080

gcgaagguug agaucacacc caauucuccc cgggccgaag cuacccuugg cggcuucggg     1140

agccugggac ucgauuguga gccuagaacc ggccuggacu uuuccgaucu uuauuaccug     1200

acaaugaaca auaaacacug gcucgugcau aaggaauggu uccacgacau uccccugccu     1260

uggcacgccg gggccgauac cggaacaccc cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggacg cccacgcgaa acgccagacg guggucgugc ucggcucuca ggagggggcu     1380

guucauaccg cccuggccgg cgcucuugag gccgaaaugg auggagcuaa gggccggcug     1440

agcuccgggc accucaagug cagacugaaa auggacaaac uucgccugaa gggagugagc     1500

uauucucucu guacagccgc guucaccuuu acaaagaucc cggccgagac ccugcauggc     1560

acggucaccg uggaaguuca guacgcuggg acagacggac cuugcaaagu gcccgcccaa     1620

auggcugucg auaugcagac ccugacaccu gugggccggc ucauuaccgc caaccccgug     1680

aucacggagu ccacagaaaa cagcaagaug augcuggagc uugacccgcc uuucggggau     1740

ucuuacaucg uuauuggcgu cggagagaag aaaaucaccc accacuggca uagauccggc     1800

agcacaauug ggaaggcguu ugaagccacc gugcgcggag cuaaacggau ggccgugcug     1860

ggcgacaccg cuugggauuu cggguccguc ggaggcgccc ucaauucucu ggggaagggc     1920

auccaccaga uuuucggagc cgcuuuuaaa agccuguucg gcgggauguc cugguuuucu     1980

caaauccuua ucggaacacu ccugaugugg cugggccuca acacgaagaa ugggagcauu     2040

ucccugaugu gucuugcgcu gggaggcgug cucaucuucc ugagcaccgc cguuucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  8905
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8905
gggagaaagc uuaccaugaa gagacggggc gccgacacca gcguggggau cgucggacug       60

cuccugacaa cggcuauggc cgcggagguu acccgcagag gcuccgcuua cuauauguac      120

cuugaucgga acgacgccgg ggaagccauu ucuuucccca caacccuggg aaugaauaaa      180

ugcuauaucc agauuaugga ucucggccac augugugacg cuacaaugag cuacgagugc      240

ccuaugcugg augaaggggu ggagccggac gauguggacu guuggugcaa caccacaucc      300

accugggucg uguauggaac gugucaucac aagaaaggcg aagcccgcag aucucggcgc      360

gcuguuaccc uucccagcca uuccacaaga aagcugcaaa cccggucuca gacauggcuc      420

gagagccgcg aguacaccaa acaccugauc agaguggaaa auuggauuuu ucggaacccu      480

ggcuucgccc uggcggccgc ugccaucgcu uggcuccugg gguccagcac gucucagaag      540

gucaucuauc uugugaugau ucugcucauc gcccccgcgu acuccauucg cugcaucgga      600

guuagcaaua gagauuuugu ggagggcaug ucugggggaa caugggucga cauugugcug      660

gaacauggcg ggugugugac cgucauggcc caggauaagc cuacaguuga caucgagcuu      720

gugaccacca caguguccaa cauggcugag guccggagcu acugcuauga agccucuauu      780

uccgauaugg cuagcgacuc ccgcuguccc acgcaaggag aggccuaccu ggacaaacag      840

ucugauaccc aguaugugug caagagaaca cucguugacc ggggcugggg caaugggugu      900

ggacuguucg gcaaagggag ccuggugacc ugcgccaagu uugcuugcuc caaaaagaug      960

acaggaaaga gcauccagcc ugaaaaccuc gaguacagaa ucaugcuguc uguccacggc     1020

ucccaacaca gcgggaugau ugugaaugau accggacaug aaacggacga gaaccgcgcg     1080

aaaguugaga ucacacccaa uucuccccgg gccgaagcua cccuuggcgg cuucgggagc     1140

cugggacucg auugugagcc uagaaccggc cuggacuuuu ccgaucuuua uuaccugaca     1200

augaacaaua agcacuggcu cgugcauaaa gaaugguucc acgacauucc ccugccuugg     1260

cacgccgggg ccgauaccgg aacaccccau uggaacaaua aggaggcucu ggucgaauuu     1320

aaagacgccc acgcgaagcg ccagacggug gucgugcucg gcucucagga gggggcuguu     1380

cauaccgccc uggccggcgc ucuugaggcc gaaauggaug gagcuaaagg ccggcugagc     1440

uccgggcacc ucaagugcag acugaagaug gacaaacuuc gccugaaggg agugagcuau     1500

ucucucugua cagccgcguu caccuuuaca aaaaucccgg ccgagacccu gcauggcacg     1560

gucaccgugg aaguucagua cgcugggaca gacggaccuu gcaaggugcc cgcccaaaug     1620

gcugucgaua ugcagacccu gacaccugug ggccggcuca uuaccgccaa ccccgugauc     1680

acggagucca cagaaaacag caaaaugaug cuggagcuug acccgccuuu cggggauucu     1740

uacaucguua uuggcgucgg agagaagaag aucacccacc acuggcauag auccggcagc     1800

acaauuggga aagcguuuga agccaccgug cgcggagcua agcggauggc cgugcugggc     1860

gacaccgcuu gggauuucgg guccgucgga ggcgcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagccgc uuuuaagagc cuguucggcg ggauguccug guuuucucaa     1980

auccuuaucg gaacacuccu gauguggcug ggccucaaca cgaaaaaugg gagcauuucc     2040

cugauguguc uugcgcuggg aggcgugcuc aucuuccuga gcaccgccgu uucugcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  8906
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8906
gggagaaagc uuaccaugag acggggcgcc gacaccagcg uggggaucgu cggacugcuc       60

cugacaacgg cuauggccgc ggagguuacc cgcagaggcu ccgcuuacua uauguaccuu      120

gaucggaacg acgccgggga agccauuucu uuccccacaa cccugggaau gaauaagugc      180

uauauccaga uuauggaucu cggccacaug ugugacgcua caaugagcua cgagugcccu      240

augcuggaug aaggggugga gccggacgau guggacuguu ggugcaacac cacauccacc      300

ugggucgugu auggaacgug ucaucacaaa aagggcgaag cccgcagauc ucggcgcgcu      360

guuacccuuc ccagccauuc cacaagaaaa cugcaaaccc ggucucagac auggcucgag      420

agccgcgagu acaccaagca ccugaucaga guggaaaauu ggauuuuucg gaacccuggc      480

uucgcccugg cggccgcugc caucgcuugg cuccuggggu ccagcacguc ucagaaaguc      540

aucuaucuug ugaugauucu gcucaucgcc cccgcguacu ccauucgcug caucggaguu      600

agcaauagag auuuugugga gggcaugucu gggggaacau gggucgacau ugugcuggaa      660

cauggcgggu gugugaccgu cauggcccag gauaagccua caguugacau cgagcuugug      720

accaccacag uguccaacau ggcugagguc cggagcuacu gcuaugaagc cucuauuucc      780

gauauggcua gcgacucccg cugucccacg caaggagagg ccuaccugga caagcagucu      840

gauacccagu augugugcaa aagaacacuc guugaccggg gcuggggcaa ugggugugga      900

cuguucggca aggggagccu ggugaccugc gccaaauuug cuugcuccaa gaaaaugaca      960

ggaaagagca uccagccuga aaaccucgag uacagaauca ugcugucugu ccacggcucc     1020

caacacagcg ggaugauugu gaaugauacc ggacaugaaa cggacgagaa ccgcgcgaag     1080

guugagauca cacccaauuc uccccgggcc gaagcuaccc uuggcggcuu cgggagccug     1140

ggacucgauu gugagccuag aaccggccug gacuuuuccg aucuuuauua ccugacaaug     1200

aacaauaaac acuggcucgu gcauaaggaa ugguuccacg acauuccccu gccuuggcac     1260

gccggggccg auaccggaac accccauugg aacaauaaag aggcucuggu cgaauuuaag     1320

gacgcccacg cgaaacgcca gacggugguc gugcucggcu cucaggaggg ggcuguucau     1380

accgcccugg ccggcgcucu ugaggccgaa auggauggag cuaagggccg gcugagcucc     1440

gggcaccuca aaugcagacu gaagauggac aaacuucgcc ugaagggagu gagcuauucu     1500

cucuguacag ccgcguucac cuuuacaaag aucccggccg agacccugca uggcacgguc     1560

accguggaag uucaguacgc ugggacagac ggaccuugca aagugcccgc ccaaauggcu     1620

gucgauaugc agacccugac accugugggc cggcucauua ccgccaaccc cgugaucacg     1680

gaguccacag aaaacagcaa gaugaugcug gagcuugacc cgccuuucgg ggauucuuac     1740

aucguuauug gcgucggaga gaaaaagauc acccaccacu ggcauagauc cggcagcaca     1800

auugggaagg cguuugaagc caccgugcgc ggagcuaaac ggauggccgu gcugggcgac     1860

accgcuuggg auuucggguc cgucggaggc gcccucaauu cucuggggaa gggcauccac     1920

cagauuuucg gagccgcuuu uaaaagccug uucggcggga uguccugguu uucucaaauc     1980

cuuaucggaa cacuccugau guggcugggc cucaacacga agaaugggag cauuucccug     2040

augugucuug cgcugggagg cgugcucauc uuccugagca ccgccguuuc ugcuugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  8907
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8907
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcgga gguuacccgg cgcggcuccg cuuacuauau guaccuugau      120

agaaacgacg ccggggaagc cauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgcuacaa ugagcuacga gugcccuaug      240

cuggaugaag ggguggagcc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaagccc ggcgcucuag acgggcuguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgagagc      420

cgggaguaca ccaagcaccu gauccgcgug gaaaauugga uuuuuagaaa cccuggcuuc      480

gcccuggcgg ccgcugccau cgcuuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgccccc gcguacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaggg caugucuggg ggaacauggg ucgacauugu gcuggaacau      660

ggcgggugug ugaccgucau ggcccaggau aagccuacag uugacaucga gcuugugacc      720

accacagugu ccaacauggc ugaggucaga agcuacugcu augaagccuc uauuuccgau      780

auggcuagcg acucccggug ucccacgcaa ggagaggccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcc aaauuugcuu gcuccaagaa aaugacagga      960

aagagcaucc agccugaaaa ccucgaguac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugaaacgg acgagaacag agcgaagguu     1080

gagaucacac ccaauucucc ccgcgccgaa gcuacccuug gcggcuucgg gagccuggga     1140

cucgauugug agccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggaaugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggccgaua ccggaacacc ccauuggaac aauaaagagg cucuggucga auuuaaggac     1320

gcccacgcga aaagacagac gguggucgug cucggcucuc aggagggggc uguucauacc     1380

gcccuggccg gcgcucuuga ggccgaaaug gauggagcua agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagccg cguucaccuu uacaaagauc ccggccgaga cccugcaugg cacggucacc     1560

guggaaguuc aguacgcugg gacagacgga ccuugcaaag ugcccgccca aauggcuguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg ccaaccccgu gaucacggag     1680

uccacagaaa acagcaagau gaugcuggag cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggcgu uugaagccac cgugagagga gcuaaacgca uggccgugcu gggcgacacc     1860

gcuugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag ccgcuuuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugcgc ugggaggcgu gcucaucuuc cugagcaccg ccguuucugc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8908
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8908
gggagaaagc uuaccauggg cgccgacacc agcgugggga ucgucggacu gcuccugaca       60

acggcuaugg ccgcggaggu uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

aacgacgccg gggaagccau uucuuucccc acaacccugg gaaugaauaa gugcuauauc      180

cagauuaugg aucucggcca caugugugac gcuacaauga gcuacgagug cccuaugcug      240

gaugaagggg uggagccgga cgauguggac uguuggugca acaccacauc caccuggguc      300

guguauggaa cgugucauca caaaaagggc gaagccagac ggucucgcag agcuguuacc      360

cuucccagcc auuccacacg gaaacugcaa acccgcucuc agacauggcu cgagagcaga      420

gaguacacca agcaccugau ccggguggaa aauuggauuu uucgcaaccc uggcuucgcc      480

cuggcggccg cugccaucgc uuggcuccug ggguccagca cgucucagaa agucaucuau      540

cuugugauga uucugcucau cgcccccgcg uacuccauua gaugcaucgg aguuagcaau      600

cgggauuuug uggagggcau gucuggggga acaugggucg acauugugcu ggaacauggc      660

ggguguguga ccgucauggc ccaggauaag ccuacaguug acaucgagcu ugugaccacc      720

acagugucca acauggcuga gguccgcagc uacugcuaug aagccucuau uuccgauaug      780

gcuagcgacu ccagaugucc cacgcaagga gaggccuacc uggacaagca gucugauacc      840

caguaugugu gcaaacggac acucguugac cgcggcuggg gcaaugggug uggacuguuc      900

ggcaagggga gccuggugac cugcgccaaa uuugcuugcu ccaagaaaau gacaggaaag      960

agcauccagc cugaaaaccu cgaguacaga aucaugcugu cuguccacgg cucccaacac     1020

agcgggauga uugugaauga uaccggacau gaaacggacg agaaccgggc gaagguugag     1080

aucacaccca auucucccag agccgaagcu acccuuggcg gcuucgggag ccugggacuc     1140

gauugugagc cucgcaccgg ccuggacuuu uccgaucuuu auuaccugac aaugaacaau     1200

aaacacuggc ucgugcauaa ggaaugguuc cacgacauuc cccugccuug gcacgccggg     1260

gccgauaccg gaacacccca uuggaacaau aaagaggcuc uggucgaauu uaaggacgcc     1320

cacgcgaaac ggcagacggu ggucgugcuc ggcucucagg agggggcugu ucauaccgcc     1380

cuggccggcg cucuugaggc cgaaauggau ggagcuaagg gcagacugag cuccgggcac     1440

cucaaaugcc gccugaagau ggacaaacuu cggcugaagg gagugagcua uucucucugu     1500

acagccgcgu ucaccuuuac aaagaucccg gccgagaccc ugcauggcac ggucaccgug     1560

gaaguucagu acgcugggac agacggaccu ugcaaagugc ccgcccaaau ggcugucgau     1620

augcagaccc ugacaccugu gggcagacuc auuaccgcca accccgugau cacggagucc     1680

acagaaaaca gcaagaugau gcuggagcuu gacccgccuu ucggggauuc uuacaucguu     1740

auuggcgucg gagagaaaaa gaucacccac cacuggcauc gcuccggcag cacaauuggg     1800

aaggcguuug aagccaccgu gcggggagcu aaaagaaugg ccgugcuggg cgacaccgcu     1860

ugggauuucg gguccgucgg aggcgcccuc aauucucugg ggaagggcau ccaccagauu     1920

uucggagccg cuuuuaaaag ccuguucggc gggauguccu gguuuucuca aauccuuauc     1980

ggaacacucc ugauguggcu gggccucaac acgaagaaug ggagcauuuc ccugaugugu     2040

cuugcgcugg gaggcgugcu caucuuccug agcaccgccg uuucugcuug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  8909
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8909
gggagaaagc uuaccauggc cgacaccagc gugggcaucg ucgggcugcu ccugacaacg       60

gcuauggccg cggagguuac cagacgggga uccgcuuacu auauguaccu ugaucgcaac      120

gacgccggcg aagccauuuc uuuccccaca acccugggga ugaauaagug cuauauccag      180

auuauggauc ucggacacau gugugacgcu acaaugagcu acgagugccc uaugcuggau      240

gaaggcgugg agccggacga uguggacugu uggugcaaca ccacauccac cugggucgug      300

uaugggacgu gucaucacaa aaagggagaa gccagacggu cucgcagagc uguuacccuu      360

cccagccauu ccacacggaa acugcaaacc cgcucucaga cauggcucga gagcagagag      420

uacaccaagc accugauccg gguggaaaau uggauuuuuc gcaacccugg cuucgcccug      480

gcggccgcug ccaucgcuug gcuccugggc uccagcacgu cucagaaagu caucuaucuu      540

gugaugauuc ugcucaucgc ccccgcguac uccauuagau gcaucggggu uagcaaucgg      600

gauuuugugg agggaauguc uggcgggaca ugggucgaca uugugcugga acauggaggc      660

ugugugaccg ucauggccca ggauaagccu acaguugaca ucgagcuugu gaccaccaca      720

guguccaaca uggcugaggu ccgcagcuac ugcuaugaag ccucuauuuc cgauauggcu      780

agcgacucca gaugucccac gcaaggggag gccuaccugg acaagcaguc ugauacccag      840

uaugugugca aacggacacu cguugaccgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucca agaaaaugac aggaaagagc      960

auccagccug aaaaccucga guacagaauc augcugucug uccacggguc ccaacacagc     1020

ggcaugauug ugaaugauac cgggcaugaa acggacgaga accgggcgaa gguugagauc     1080

acacccaauu cucccagagc cgaagcuacc cuuggaggcu ucggcagccu ggggcucgau     1140

ugugagccuc gcaccggacu ggacuuuucc gaucuuuauu accugacaau gaacaauaaa     1200

cacuggcucg ugcauaagga augguuccac gacauucccc ugccuuggca cgccggcgcc     1260

gauaccggga caccccauug gaacaauaaa gaggcucugg ucgaauuuaa ggacgcccac     1320

gcgaaacggc agacgguggu cgugcucgga ucucaggagg gcgcuguuca uaccgcccug     1380

gccggggcuc uugaggccga aauggauggc gcuaagggaa gacugagcuc cggccaccuc     1440

aaaugccgcc ugaagaugga caagcugcgg cugaaagggg ugagcuauuc ucucuguaca     1500

gccgcguuca ccuuuacaaa gaucccggcc gagacccugc auggaacggu caccguggaa     1560

guucaguacg cuggcacaga cgggccuugc aaagugcccg cccaaauggc ugucgauaug     1620

cagacccuga caccuguggg aagacucauu accgccaacc ccgugaucac ggaguccaca     1680

gaaaacagca agaugaugcu ggagcuugac ccgccuuucg gcgauucuua caucguuauu     1740

ggggucggcg agaaaaagau cacccaccac uggcaucgcu ccggaagcac aauuggcaag     1800

gcguuugaag ccaccgugag aggggcuaaa cggauggccg ugcugggaga caccgcuugg     1860

gauuucggcu ccgucggggg agcccucaau ucucugggca aggggaucca ccagauuuuc     1920

ggcgccgcuu uuaaaagccu guucggaggc auguccuggu uuucucaaau ccuuaucggg     1980

acacuccuga uguggcuggg acucaacacg aagaauggca gcauuucccu gaugugucuu     2040

gcgcuugggg gagugcucau cuuccugagc accgccguuu cugcuugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  8910
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8910
gggagaaagc uuaccaugga caccagcgug ggcaucgucg ggcugcuccu gacaacggcc       60

auggcugccg agguuaccag acggggaucc gcguacuaua uguaccuuga ucgcaacgac      120

gcuggcgaag ccauuucuuu ccccacaacc cuggggauga auaagugcua uauccagauu      180

auggaucucg gacacaugug ugacgccaca augagcuacg agugcccuau gcuggaugaa      240

ggcguggagc cggacgaugu ggacuguugg ugcaacacca cauccaccug ggucguguau      300

gggacguguc aucacaaaaa gggagaagcu agacggucuc gcagagccgu uacccuuccc      360

agccauucca cacggaaacu gcaaacccgc ucucagacau ggcucgagag cagagaguac      420

accaagcacc ugauccgggu ggaaaauugg auuuuucgca acccuggcuu cgcucuggcc      480

gcggccgcua ucgccuggcu ccugggcucc agcacgucuc agaaagucau cuaucuugug      540

augauucugc ucaucgcucc cgccuacucc auuagaugca ucgggguuag caaucgggau      600

uuuguggagg gaaugucugg cgggacaugg gucgacauug ugcuggaaca uggaggcugu      660

gugaccguca uggcgcagga uaagccuaca guugacaucg agcuugugac caccacagug      720

uccaacaugg ccgagguccg cagcuacugc uaugaagcua gcauuuccga uauggccagc      780

gacuccagau gucccacgca aggggaggcu uaccuggaca agcagucuga uacccaguau      840

gugugcaaac ggacacucgu ugaccgcgga uggggcaaug gcugugggcu guucggaaag      900

ggcagccugg ugaccugcgc caaauuugcc ugcuccaaga aaaugacagg gaagucuauc      960

cagccugaaa accucgagua cagaaucaug cugucugucc acggauccca acacagcggc     1020

augauuguga augauaccgg gcaugaaacg gacgagaacc gggcuaaggu ugagaucaca     1080

cccaauucuc ccagagcgga agccacccuu ggaggcuucg gcagccuggg gcucgauugu     1140

gagccucgca ccggacugga cuuuuccgau cuuuauuacc ugacaaugaa caauaaacac     1200

uggcucgugc auaaggaaug guuccacgac auuccccugc cuuggcacgc uggcgccgau     1260

accgggacac cccauuggaa caauaaagag gcccuggucg aauuuaagga cgcucacgcc     1320

aaacggcaga cgguggucgu gcucggaucu caggagggcg cgguucauac cgcucuggcc     1380

ggggcccuug aggcugaaau ggauggcgcc aagggaagac ugagcuccgg ccaccucaaa     1440

ugccgccuga agauggacaa gcugcggcug aaagggguga gcuauucucu cuguacagcu     1500

gccuucaccu uuacaaagau cccggcggag acccugcaug gaacggucac cguggaaguu     1560

caguacgccg gcacagacgg gccuugcaaa gugcccgcuc aaauggccgu cgauaugcag     1620

acccugacac cugugggaag acucauuacc gcuaaccccg ugaucacgga guccacagaa     1680

aacagcaaga ugaugcugga gcuugacccg ccuuucggcg auucuuacau cguuauuggg     1740

gucggcgaga aaaagaucac ccaccacugg caucgcuccg gaagcacaau uggcaaggcc     1800

uuugaagcga ccgugagagg ggccaaacgg auggcugugc ugggagacac cgccugggau     1860

uucggcuccg ucgggggagc ucucaauucu cugggcaagg ggauccacca gauuuucggc     1920

gccgccuuua aaagccuguu cggaggcaug uccugguuuu cucaaauccu uaucgggaca     1980

cuccugaugu ggcugggacu caacacgaag aauggcagca uuucccugau gugucuugcu     2040

cuugggggag ugcucaucuu ccugagcacc gcgguuucug ccugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  8911
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  8911
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8912
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8912
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgcgg uuaccagacg gggauccgcu uacuauaugu accuugaccg caacgaugcc      120

ggcgaggcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugcuacaaug agcuacgaau gcccuaugcu ggacgagggc      240

guggaaccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaggccaga cggucucgca gagcuguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgaaagcag agaguacacc      420

aagcaccuga uccgggugga gaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggaaggaa ugucuggcgg gacauggguc gauauugugc uggagcaugg aggcugugug      660

accgucaugg cccaggacaa gccuacaguu gauaucgaac uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaggccucua uuuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaagccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauccag      960

ccugagaacc ucgaauacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugagacggau gaaaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaggc uacccuugga ggcuucggca gccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccccugccuu ggcacgccgg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaagcu cuggucgagu uuaaggaugc ccacgcgaaa     1320

cggcagacgg uggucgugcu cggaucucag gaaggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgagaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgaaacc cugcauggaa cggucaccgu ggagguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaggccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8913
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8913
gggagaaagc uuaccaugag aggcgccgac accagcgugg ggaucgucgg acugcuccug       60

acaacggcua uggccgcggc uguuacccgg cgcggcuccg ccuacuauau guaccuugau      120

agaaacgacg ccggggaggc uauuucuuuc cccacaaccc ugggaaugaa uaagugcuau      180

auccagauua uggaucucgg ccacaugugu gacgccacaa ugagcuacga augcccuaug      240

cuggaugagg ggguggaacc ggacgaugug gacuguuggu gcaacaccac auccaccugg      300

gucguguaug gaacguguca ucacaaaaag ggcgaggcuc ggcgcucuag acgggccguu      360

acccuuccca gccauuccac acgcaaacug caaaccagau cucagacaug gcucgaaagc      420

cgggaguaca ccaagcaccu gauccgcgug gagaauugga uuuuuagaaa cccuggcuuc      480

gcgcuggccg cugccgcuau cgccuggcuc cuggggucca gcacgucuca gaaagucauc      540

uaucuuguga ugauucugcu caucgcgccc gccuacucca uucggugcau cggaguuagc      600

aaucgcgauu uuguggaagg caugucuggg ggaacauggg ucgacauugu gcuggagcau      660

ggcgggugug ugaccgucau ggcucaggau aagccuacag uugacaucga acuugugacc      720

accacagugu ccaacauggc cgaggucaga agcuacugcu augaggcuuc uauuuccgau      780

auggccagcg acucccggug ucccacgcaa ggagaagccu accuggacaa gcagucugau      840

acccaguaug ugugcaaacg cacacucguu gacagaggcu ggggcaaugg guguggacug      900

uucggcaagg ggagccuggu gaccugcgcu aaauuugcgu gcuccaagaa aaugacagga      960

aagagcaucc agccugagaa ccucgaauac cggaucaugc ugucugucca cggcucccaa     1020

cacagcggga ugauugugaa ugauaccgga caugagacgg acgaaaacag agccaagguu     1080

gagaucacac ccaauucucc ccgcgcugag gccacccuug gcggcuucgg gagccuggga     1140

cucgauugug aaccucggac cggccuggac uuuuccgauc uuuauuaccu gacaaugaac     1200

aauaaacacu ggcucgugca uaaggagugg uuccacgaca uuccccugcc uuggcacgcc     1260

ggggcugaua ccggaacacc ccauuggaac aauaaagaag cccuggucga guuuaaggac     1320

gcgcacgcua aaagacagac gguggucgug cucggcucuc aggaaggggc cguucauacc     1380

gcccuggcug gcgcccuuga ggcugagaug gauggagcca agggccgccu gagcuccggg     1440

caccucaaau gccggcugaa gauggacaaa cuuagacuga agggagugag cuauucucuc     1500

uguacagcgg ccuucaccuu uacaaagauc ccggcugaaa cccugcaugg cacggucacc     1560

guggagguuc aguacgccgg gacagacgga ccuugcaaag ugcccgcuca aauggccguc     1620

gauaugcaga cccugacacc ugugggccgc cucauuaccg cgaaccccgu gaucacggaa     1680

uccacagaga acagcaagau gaugcuggaa cuugacccgc cuuucgggga uucuuacauc     1740

guuauuggcg ucggagagaa aaagaucacc caccacuggc aucgguccgg cagcacaauu     1800

gggaaggccu uugaggcuac cgugagagga gccaaacgca uggcugugcu gggcgacacc     1860

gccugggauu ucggguccgu cggaggcgcc cucaauucuc uggggaaggg cauccaccag     1920

auuuucggag cugcguuuaa aagccuguuc ggcgggaugu ccugguuuuc ucaaauccuu     1980

aucggaacac uccugaugug gcugggccuc aacacgaaga augggagcau uucccugaug     2040

ugucuugccc ugggaggcgu gcucaucuuc cugagcaccg cuguuucugc cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  8914
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8914
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gaggcguuac gcggcgcggg      120

agcgccuacu auauguaccu ggauagaaac gacgcuggag aggccauuuc cuuucccacc      180

acacuuggca ugaauaaaug cuauauccaa auuauggauc uggggcauau gugugacgcg      240

accaugucuu acgaaugccc uaugcucgau gagggagugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggcaccu gucaccauaa gaaaggcgag      360

gcucggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg guuugcccuu gcugccgcug ccaucgcgug gcugcucgga      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ccccgcuuac      600

ucuauucggu gcaucggcgu uuccaaucgc gauuucgugg aagggaugag cggaggcacg      660

ugggucgaca uugugcucga gcacggggga ugugugacag ucauggccca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggcugaggu cagauccuac      780

ugcuaugagg ccagcauuuc cgauauggcg ucugacagcc ggugucccac acagggcgaa      840

gccuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggga auggaugugg ccuguuuggg aaaggaagcc uugugacaug cgcuaaguuc      960

gccugcucua aaaagaugac cggcaagucc auccaaccug agaaccugga auaccggauc     1020

augcucagcg uccacggguc ucagcauagc ggaaugauug ugaaugauac aggccacgag     1080

accgacgaaa acagagcuaa aguugagauc acgcccaauu ccccgcgcgc cgaggccaca     1140

cugggcgggu uuggaucucu uggccuggau ugugaaccuc ggaccgggcu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcuggagcg gauacaggca caccccacug gaacaauaag     1320

gaagcccuug ucgaguucaa agacgcucau gccaagagac agacaguggu cgugcugggg     1380

ucccaggaag gcgccguuca cacggcucuc gccggagcgc uggaggcuga gauggauggc     1440

gccaaagggc gccuuagcuc uggacaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggcg uguccuauag ccucuguacc gccgcuuuua cauucaccaa aaucccggcc     1560

gaaacacugc acgggaccgu cacgguggag guucaauacg cuggaaccga cggcccuugc     1620

aaggugcccg cccagauggc ggucgauaug cagacacuua ccccuguggg gcgccugauu     1680

acagccaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gcgacagcua caucguuauu ggagucggcg agaagaagau cacacaccau     1800

uggcaccggu ccgggucuac cauuggaaag gcuuucgagg ccacagugag aggcgcuaaa     1860

cgcauggccg ugcuggggga caccgcgugg gauuucggaa gcgucggcgg ggcccuuaau     1920

ucccucggca aaggaaucca ucaaauuuuu ggcgcugccu ucaagucucu guuuggggga     1980

augagcuggu ucucccagau ccugaucggc acccuccuga uguggcuugg gcugaacacc     2040

aaaaauggaa gcauuucucu caugugucug gcucugggcg gggugcuuau cuuucucucc     2100

acggccguua gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8915
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  8915
gggagaaagc uuaccaugag aggccugagc ucccggaagc gcagaucuca cgacgugcuc       60

accguccagu uccugauccu ugggaugcug cucaugacag gagccguuac gcggcgcggc      120

agcgcuuacu auauguaccu ggauagaaac gacgccgggg aggcgauuuc cuuucccacc      180

acacuuggaa ugaauaaaug cuauauccaa auuauggauc ugggccauau gugugacgcu      240

accaugucuu acgaaugccc uaugcucgau gagggggugg aaccggacga uguggacugu      300

uggugcaaca caaccagcac augggucgug uauggaaccu gucaccauaa gaaaggcgag      360

gcccggcgcu ccagacgggc cguuacgcug cccucucaca gcacccgcaa gcugcagaca      420

agaucccaga ccuggcucga aagccgggag uacacaaaac aucugauccg cguggagaau      480

uggauuuuca gaaacccugg cuuugcucuu gccgcugccg cgaucgccug gcugcucggg      540

ucuuccacca gccagaaggu caucuaucug gugaugauuc uucugaucgc ucccgccuac      600

ucuauucggu gcaucggagu uuccaaucgc gauuucgugg aaggcaugag cgggggaacg      660

ugggucgaca uugugcucga gcacggcggg ugugugacag ucauggcuca agauaagccu      720

accguugaca ucgaacuggu gacaaccacc gugucuaaca uggccgaggu cagauccuac      780

ugcuaugagg cgagcauuuc cgauauggcc ucugacagcc ggugucccac acagggagag      840

gcuuaccugg acaaacaguc cgauacgcag uaugugugca agagaacccu cguugaccgc      900

ggcuggggca augggugugg acuguuuggc aaagggagcc uugugacaug cgccaaguuc      960

gcuugcucua aaaagaugac cggaaagucc auccaaccug aaaaccugga auaccggauc     1020

augcucagcg uccacggcuc ucagcauagc gggaugauug ugaaugauac aggacacgag     1080

accgacgaaa acagagccaa aguugagauc acgcccaauu ccccgcgcgc cgaggcuaca     1140

cugggcggcu uugggucucu uggacuggau ugugaaccuc ggaccggccu cgacuucagc     1200

gaucuguauu accugaccau gaacaauaag cauuggcucg ugcacaaaga gugguuucac     1260

gacauucccc ugccuuggca ugcgggggcc gauacaggaa caccccacug gaacaauaag     1320

gaagcucuug ucgaguucaa agacgcccau gccaagagac agacaguggu cgugcugggc     1380

ucccaggaag gggcuguuca cacggcccuc gcgggcgcuc uggaggccga gauggaugga     1440

gccaaaggcc gccuuagcuc ugggcaucug aagugccggc ucaagaugga caaacugaga     1500

cugaagggag uguccuauag ccucuguacc gcugccuuua cauucaccaa aaucccggcu     1560

gaaacacugc acggcaccgu cacgguggag guucaauacg ccgggaccga cggaccuugc     1620

aaggugcccg cgcagauggc cgucgauaug cagacacuua ccccuguggg ccgccugauu     1680

acagcuaacc ccgugaucac cgaaucuacg gagaacucca aaaugaugcu cgaacuggau     1740

cccccuuuug gggacagcua caucguuauu ggcgucggag agaagaagau cacacaccau     1800

uggcaccggu ccggcucuac cauugggaaa gccuucgagg cuacagugag aggagccaag     1860

cgcauggcgg ugcugggcga caccgccugg gauuucggga gcgucggagg cgcucuuaau     1920

ucccucggga aaggcaucca ucaaauuuuu ggagccgcuu ucaagucucu guuuggcggg     1980

augagcuggu ucucccagau ccugaucgga acccuccuga uguggcuugg ccugaacacc     2040

aaaaauggga gcauuucucu caugugucug gcccugggag gcgugcuuau cuuucucucc     2100

acggccguua gcgcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  8916
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 3 opt

<400>  8916
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacau ugugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8917
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 3 opt

<400>  8917
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaca      240

ugugacgcca ccaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacacuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaucucagac cuggcucgag agccgggaau acacgaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacauc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcucag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacc      840

caaggagaag ccuaccugga caaacagucu gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcuaaguuug cgugcuccaa gaaaaugacc ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauacg     1080

ggacaugaga cagacgagaa uagagccaaa guggaaauua ccccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auacaggaac gccccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acagcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacgcugca uggcaccgug acaguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cggcgaaccc cguuaucaca gaauccaccg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac acagccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaaugcca agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca ccgccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8918
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 3 opt

<400>  8918
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgg auacgaaacg     1080

gaugaggaca gagcuaaggu ggaggucaca ccuaauagcc cgcgcgcgga agccacccuu     1140

ggcggcuucg ggucccuggg acucgauugu gagccccgga ccggccugga cuuuucugau     1200

cuuuauuacc ugacaaugaa caauaaacac uggcucgugc auaaggaaug guuccacgac     1260

auuccucugc ccuggcacgc uggggccgau accggaacac cucauuggaa caauaaagag     1320

gcccuggucg aauuuaagga cgcucacgcc aagagacaga cggugguugu gcucggcagc     1380

caggaggggg cgguccauac cgcucuggcc ggcgcccuug aggcugaaau ggauggagcc     1440

aaaggccgcc uguucuccgg gcaccucaag ugccggcuga aaauggacaa acuuagacug     1500

aagggaguga gcuauucucu cuguacagcu gccuuuaccu ucacaaaggu ucccgcggag     1560

acccugcaug gcacggugac cgucgaagug caguacgccg ggacagacgg accgugcaag     1620

aucccugugc aaauggcugu ugauaugcag acccugacac ccgucggccg ccucaucacc     1680

gccaacccug ugauuacgga guccacagaa aauagcaaaa ugaugcugga gcuugacccu     1740

cccuuugggg auucuuacau cgugauuggc gucggagaca agaaaaucac ccaccacugg     1800

caucgguccg gcagcacaau ugggaaggcu uucgaggcca ccgugagagg agcgaaacgc     1860

auggccguuc ugggcgauac cgcuugggac uucggguccg ugggaggcgu cuuuaacucu     1920

cucgggaagg gcauccacca gaucuucgga gccgcuuuua agagccuguu cggcgggaug     1980

uccugguuuu cucaaauucu gaucggaaca cuucucgugu ggcugggccu gaacacgaaa     2040

aaugggagca uuucccucac cugucuggcc cuuggaggcg uuaugaucuu ccugagcaca     2100

gccgugucug cugaugucgg gugcuccgug gacuuuagca agugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8919
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 3 opt

<400>  8919
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8920
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 3 opt

<400>  8920
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgu gaaugauacg     1080

ggauacgaaa cagacgagaa cagagcuaag gucgagguga ccccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaaac acuggcucgu ccauaaggaa     1260

ugguuccacg acauuccucu gcccuggcac gcuggggccg auacaggaac gccucauugg     1320

aacaauaaag aggcccuggu ggaauuuaag gacgcucacg ccaagagaca gaccguugug     1380

guccucggca gccaggaggg ggcggugcau acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggcaa gcuguucucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuucggc ugaagggagu uagcuauucu cucuguaccg cugccuuuac auucaccaag     1560

gugcccgcgg agacgcugca uggcaccguc acaguggaag ugcaguacgc cgggaccgac     1620

ggaccgugca aaaucccugu ucaaauggcu gucgauaugc agacacugac ccccgugggc     1680

agacucauca cggccaaccc ugugauuaca gaguccaccg aaaacagcaa gaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgucauug gcgugggaga caaaaagauc     1800

acacaccacu ggcaucgcuc cggcagcacc auugggaagg cuuucgaggc caccguucgg     1860

ggagcgaaaa gaauggccgu gcugggcgau acagcuuggg acuucggguc cgucggaggc     1920

guguuuaauu cucucgggaa gggcauccac cagaucuucg gagccgcuuu uaaaagccug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cgcuucucgu uuggcugggc     2040

cugaacacca agaaugggag cauuucccuc acaugucugg cccuuggagg cgugaugauc     2100

uuccugagca ccgccgucuc ugcugaugug gggugcuccg uugacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8921
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 3 opt

<400>  8921
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucacgcugg ucgccguggc uaucgccugg      540

cuccugggga gcucuacauc ccagaagguu auuuaccuug ugaugauccu gcucauugcu      600

ccugccuaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaaccu ggguggacgu cguucuggaa cacggcgggu gugugacagu gauggcgcag      720

gauaaaccca ccgucgacau cgagcuugug accacaacgg uuagcaacau ggccgaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacc      840

caaggagagg cuuaucugga caagcagagc gauacacagu acgucugcaa aagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuacaugc      960

gccaaguuua ccugcagcaa aaagaugacg ggaaaaucua uucagcccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc caacauucug ggaugauugu caaugaugaa     1080

aacagagcca agguggaggu cacaccuaau agcccgcgcg cugaggcgac ccuuggaggc     1140

uucggcuccc uggggcucga cugugaaccc cggaccggac uggauuuuuc ugaccuuuac     1200

uaucugacaa ugaacaauaa acacuggcuc gugcauaagg agugguucca cgauaucccu     1260

cugcccuggc acgccggcgc ugacaccggg acaccucauu ggaacaauaa agaagcccug     1320

guugaguuua aggaugccca cgcuaagaga cagacggugg ucgugcucgg aagccaggaa     1380

ggcgccguuc auaccgcgcu ggcuggggcc cuugaggccg agauggacgg cgcuaaagga     1440

cgccuguucu ccggccaccu caagugccgg cugaaaaugg auaaacuuag acugaagggg     1500

gugagcuacu cucucuguac agccgcuuuu accuucacaa agguccccgc cgaaacccug     1560

cauggaacgg ugaccgugga gguucaguau gcgggcacag acgggccgug caaggucccu     1620

gcccaaaugg cuguggacau gcagacccug acacccgugg gacgccucau uaccgccaac     1680

ccugucauca cggaauccac agagaacagc aaaaugaugc uggaacuuga uccucccuuu     1740

ggcgacucuu acaucgugau ugggguuggc gauaagaaaa ucacccacca cuggcaucgg     1800

uccggaagca caauuggcaa ggcuuucgag gccaccguga gaggggcgaa acgcauggcc     1860

guccugggag acaccgcuug ggauuucggc uccguugggg gaguguuuaa uucucucggc     1920

aaggggaucc accagauuuu cggcgccgcu uuuaagagcc uguucggagg cauguccugg     1980

uuuucucaaa uccugaucgg gacacuucuc guguggcugg gacugaacac gaaaaauggc     2040

agcauuuccc ucaccugucu ggcccuuggg ggagucauga ucuuccugag cacagccgug     2100

ucugcugacg uuggcugcuc cguggauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8922
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 3 opt

<400>  8922
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccgcaag      360

aaaggcgaag ccagacggag ccgcagagcg gugacccuuc cgucccauuc uacacggaag      420

cugcaaaccc gcagccagac auggcucgag uccagagagu auaccaagca ccugauuaaa      480

guugaaaauu ggaucuuucg gaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucgcug caucggagug ucuaauagag auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cauggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

cggucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucuag auguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa acgcacacuc      900

gucgaccggg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uauagaauua ugcuguccgu ucacggcagc caacacucug ggaugaucgu gaaugauacg     1080

ggauacgaaa cagacgagaa ccgcgcuaag gucgagguga ccccuaauag cccgcgggcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagcccag aacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaaac auuggcucgu ccacaaggaa     1260

ugguuccaug acauuccucu gcccuggcac gcuggggccg auacaggaac gccucacugg     1320

aacaauaaag aggcccuggu ggaauuuaag gacgcucaug ccaagcgcca gaccguugug     1380

guccucggca gccaggaggg ggcggugcac acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggcaa gcuguucucc gggcaucuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu uagcuauucu cucuguaccg cugccuuuac auucaccaag     1560

gugcccgcgg agacgcugca cggcaccguc acaguggaag ugcaguacgc cgggaccgac     1620

ggaccgugca aaaucccugu ucaaauggcu gucgauaugc agacacugac ccccgugggc     1680

cgccucauca cggccaaccc ugugauuaca gaguccaccg aaaacagcaa gaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgucauug gcgugggaga caaaaagauc     1800

acacaucacu ggcaccgguc cggcagcacc auugggaagg cuuucgaggc caccguuaga     1860

ggagcgaaac gcauggccgu gcugggcgau acagcuuggg acuucggguc cgucggaggc     1920

guguuuaauu cucucgggaa gggcauccau cagaucuucg gagccgcuuu uaaaagccug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cgcuucucgu uuggcugggc     2040

cugaacacca agaaugggag cauuucccuc acaugucugg cccuuggagg cgugaugauc     2100

uuccugagca ccgccgucuc ugcugaugug gggugcuccg uugacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8923
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 3 opt

<400>  8923
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugacggu gaaugauauc     1080

ggauacgaaa cagacgagaa cagagcuaag gucgagguga ccccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaaac acuggcucgu ccauaaggaa     1260

ugguuccacg acauuccucu gcccuggcac gcuggggccg auacaggaac gccucauugg     1320

aacaauaaag aggcccuggu ggaauuuaag gacgcucacg ccaagagaca gaccguugug     1380

guccucggca gccaggaggg ggcggugcau acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggcaa gcuguucucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuucggc ugaagggagu uagcuauucu cucuguaccg cugccuuuac auucaccaag     1560

gugcccgcgg agacgcugca uggcaccguc acaguggaag ugcaguacgc cgggaccgac     1620

ggaccgugca aaaucccugu ucaaauggcu gucgauaugc agacacugac ccccgugggc     1680

agacucauca cggccaaccc ugugauuaca gaguccaccg aaaacagcaa gaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgucauug gcgugggaga caaaaagauc     1800

acacaccacu ggcaucgcuc cggcagcacc auugggaagg cuuucgaggc caccguucgg     1860

ggagcgaaaa gaauggccgu gcugggcgau acagcuuggg acuucggguc cgucggaggc     1920

guguuuaauu cucucgggaa gggcauccac cagaucuucg gagccgcuuu uaaaagccug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cgcuucucgu uuggcugggc     2040

cugaacacca agaaugggag cauuucccuc acaugucugg cccuuggagg cgugaugauc     2100

uuccugagca ccgccgucuc ugcugaugug gggugcuccg uugacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8924
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 3 opt

<400>  8924
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgg auacgaaacg     1080

gaugagaaua gagcuaaggu ggaggucaca ccuaacagcc cgcgcgcgga agccacccuu     1140

ggcggcuucg ggucccuggg acucgacugu gagccccgga ccggccugga uuuuucugac     1200

cuuuauuacc ugacaaugaa uaacaaacac uggcucgugc auaaggaaug guuccacgau     1260

auuccucugc ccuggcacgc uggggccgac accggaacac cucauuggaa uaacaaagag     1320

gcccuggucg aauuuaagga ugcucacgcc aagagacaga cggugguugu gcucggcagc     1380

caggaggggg cgguccauac cgcucuggcc ggcgcccuug aggcugaaau ggacggagcc     1440

aaaggcaagc uguucuccgg gcaccucaaa ugccgccuga agauggauaa acuucggcug     1500

aagggaguga gcuauucucu cuguacagcu gccuuuaccu ucacaaaggu ucccgcggag     1560

acccugcaug gcacggugac cgucgaagug caguacgccg ggacagacgg accgugcaaa     1620

aucccugugc aaauggcugu ugacaugcag acccugacac ccgucggcag acucaucacc     1680

gccaauccug ugauuacgga guccacagaa aacagcaaga ugaugcugga gcuugauccu     1740

cccuuugggg acucuuacau cgugauuggc gucggagaua aaaagaucac ccaccacugg     1800

caucgcuccg gcagcacaau ugggaaggcu uucgaggcca ccgugcgggg agcgaaaaga     1860

auggccguuc ugggcgacac cgcuugggau uucggguccg ugggaggcgu cuuuaacucu     1920

cucgggaagg gcauccacca gaucuucgga gccgcuuuua aaagccuguu cggcgggaug     1980

uccugguuuu cucaaauucu gaucggaaca cuucucgugu ggcugggccu gaauacgaag     2040

aacgggagca uuucccucac cugucuggcc cuuggaggcg uuaugaucuu ccugagcaca     2100

gccgugucug cugacgucgg gugcuccgug gauuuuagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  8925
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 3 opt

<400>  8925
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggcg gggcauucac cagaucuucg gagcugcguu uaaaagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8926
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 3 opt

<400>  8926
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacgccagcg uggggaucgu cggacugcuc cugaccacag cgauggcugc cgaaguuacg      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgcugggga ggccauuucu      180

uuccccacca cacugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccaca ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcuc uagacgggcg guuacgcuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaucucagac cuggcucgag agccgggaau acacaaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcccugg cugccgcugc cauugcgugg      540

cuccuggggu ccagcaccuc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcuuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacgu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug acaaccaccg ucuccaauau ggcugaagug      780

agaagcuacu gcuaugaggc cucuaucucc gauauggcca gcgacucccg gugucccaca      840

caaggagagg cuuaccugga caaacagucu gauacgcagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcgaaguuug ccugcuccaa gaaaaugacc ggaaagagca uccagccuga aaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauaca     1080

ggacaugaga ccgacgagaa uagagcuaaa guggaaauua cgccgaacuc uccccgcgcc     1140

gaggccacac uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gcuggggccg auacaggaac accccauugg     1320

aauaacaagg aagcgcuggu ggaguuuaaa gacgcucacg ccaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acggcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg ccgcuuucac auuuaccaaa     1560

auuccggccg aaacacugca uggcaccgug acgguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcg guggauaugc agacacugac cccugugggc     1680

cgccucauca cagccaaccc cguuaucacc gaauccacgg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcucucaacu cucuggggaa aggcauucac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaauacaa agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca cggccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8927
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 3 opt

<400>  8927
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc cuggcgcucg ccgcugccgc uauugccugg      540

cugcuugggu ccagcacguc ucagaaaguc aucuaucugg ugaugauucu ccugaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuucgugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuugag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuggug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccucga caaacagucu gauacccagu auguuugcaa gagaacacug      900

guggaccgcg gcuggggcaa cgggugugga cuguuuggca aagggagccu cgugaccugc      960

gcuaaguucg cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucuggaa     1020

uaccggauua ugcuuucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc ugggcggcuu ugggagccuc ggacuggauu gugaaccucg gaccggccuu     1200

gacuucuccg aucuguauua ccucacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuucacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccucgu ggaguucaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucugggcu cucaggaggg ggccgugcau accgcccuug cuggcgcccu ggaagcugag     1440

auggauggag ccaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggac     1500

aaacugagac ucaagggagu cagcuauucu cuguguacag cggccuuuac cuucacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccucac accugugggc     1680

cgccugauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggauc ccccuuuugg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuucgaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccucggcgac accgccuggg auuuuggguc cgugggaggc     1920

gcccugaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu caagagccuu     1980

uuuggcggga uguccugguu cucucaaauc cucauuggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag caucucccuu augugucugg cccucggagg cguucugauu     2100

uuucugagca ccgcuguguc ugccgacguc gggugcuccg uggauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8928
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 3 opt

<400>  8928
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu gguaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucau cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8929
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 3 opt

<400>  8929
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaaauc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8930
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 3 opt

<400>  8930
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccc augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccuggc ucaaauggcc guggauaugc agacccucac acccgugggc     1680

cgccugauca ccgcgaaccc uguuaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggacc cucccuucgg ggauucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccucggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccugaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccuu     1980

uucggcggga uguccugguu uucucaaauc cucauuggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag caucucccuu augugucugg cccucggagg cguucugauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8931
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 3 opt

<400>  8931
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu acccuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacacuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaucucagac cuggcucgag agccgggaau acacgaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacauc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcucag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacc      840

caaggagaag ccuaccugga caaacagucu gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcuaaguuug cgugcuccaa gaaaaugacc ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauacg     1080

ggacaugaga cagacgagaa uagagccaaa guggaaauua ccccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auacaggaac gccccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acagcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacgcugca uggcaccgug acaguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cggcgaaccc cguuaucaca gaauccaccg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac acagccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaauacca agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca cagcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8932
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 3 opt

<400>  8932
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccguggucuc ugcugacgug gggugcuccg uugauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8933
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 3 opt

<400>  8933
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccgcu ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcuc uagacgggcg guuacgcuuc ccagccauuc cacccgcaag      420

cugcaaacaa gaccucagac cuggcucgag ucucgggaau acacaaagca ccugauccgc      480

guggagaacu ggaucuuuag aaaucccggc uucgcccugg cugccgcugc cauugcgugg      540

cuccugggga gcuccaccag ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

ccugcuuacu cuauucggug caucggaguu uccaaccgcg auuuugugga aggcaugagc      660

gggggaacgu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccca ccguggacau ugagcuugug acaaccaccg ucucuaauau ggcugaagug      780

agauccuacu gcuaugaggc cagcaucucu gauauggccu ccgacagccg guguccuaca      840

caaggagagg cuuaccugga caaacagucc gauacgcagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggucucu ggucacaugc      960

gcgaaguuug ccugcagcaa gaaaaugacc ggaaagucca uccagccgga gaaucucgaa     1020

uaccggauua ugcugagcgu gcacggcucu caacacuccg ggaugaucgu uaacgauaca     1080

ggacaugaga ccgacgagaa uagagcuaaa guggaaauua cgcccaacag cccucgcgcc     1140

gaggccacac uuggcggcuu cgggucucug ggacucgauu gugaaccccg gaccggccug     1200

gacuuuagcg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccucu gcccuggcac gcuggggccg auacaggaac cccgcauugg     1320

aauaacaagg aagcgcuggu ggaguuuaaa gacgcucacg ccaagagaca gacagucgug     1380

guucucggcu cccaggaggg ggccgugcau acggcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguaccg ccgcuuucac auuuaccaaa     1560

auuccugccg aaacacugca uggcaccgug acgguugagg ugcaguacgc ugggaccgac     1620

ggacccugca aggucccugc ccaaauggcg guggauaugc agacacugac ccccgugggc     1680

cgccucauca cagccaaccc cguuaucacc gaaucuacgg aaaauuccaa gaugaugcug     1740

gagcuugacc cuccguucgg ggauagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc uggcuccacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcucucaacu cccuggggaa aggcauucac cagaucuucg gagcggccuu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaauacaa agaacggguc uaucagccug augugucuug cucugggagg cguucucauu     2100

uuccugucca cggccgugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8934
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 3 opt

<400>  8934
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc uaaaaagguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaaaccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

cagggagaag ccuaccugga caagcaaucu gauacccagu auguuugcaa aagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aggggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa aaagaugaca ggaaaaagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc cagcacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaag guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaaac acuggcucgu ccauaaggag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaaag aagcccuggu ggaguuuaag gacgcgcacg cuaagagaca aacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaaaggccg ccugagcucc gggcaccuca agugccggcu gaaaauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaag     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucagauggcc guggauaugc aaacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa aaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga aaagaaaauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaagg ccuuugaggc uaccgugaga     1860

ggagccaaac gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa gggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucagauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga aaaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8935
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 3 opt

<400>  8935
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacgcg gcagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cuauucgcug caucggaguu uccaacagag auuuugugga aggcaugagc      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucucuaauau ggccgaagug      780

cgguccuacu gcuaugaggc uagcaucucu gauauggccu ccgacagcag augucccacg      840

caaggagaag ccuaccugga caaacagucc gauacccagu auguuugcaa gcgcacacuc      900

guggaccggg gcuggggcaa cgggugugga cuguucggca aagggucucu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggaaagucca uccagccuga gaaucucgaa     1020

uacagaauua ugcugagcgu ccacggcucu caacacuccg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa ucgcgccaaa guggaaauua caccgaacag cccccgggcu     1140

gaggccaccc uuggcggcuu cgggucucug ggacucgauu gugaaccuag aaccggccug     1200

gacuuuagcg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagcgcca gacggucgug     1380

guucucggcu cccaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg gcugucuagc gggcaccuca aaugcagacu gaagauggac     1500

aaacuucgcc ugaagggagu cuccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cggcucauca ccgcgaaccc cguuaucacg gaaucuacag aaaauuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcauagauc uggcuccaca aucgggaaag ccuuugaggc uaccgugcgc     1860

ggagccaagc ggauggcugu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcccucaacu cccuggggaa aggcauucac cagaucuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacggguc uaucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucca ccgcugugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8936
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 3 opt

<400>  8936
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaagcgcu       60

gacaccuccg ugggcaucgu cgggcugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggau cugccuacua uauguaccuu gauagaaaug acgccggcga ggcuauuagc      180

uuccccacaa cccuggggau gaacaaaugc uauauccaga uuauggaucu cggacacaug      240

ugugacgcca caauguccua cgaaugcccu augcuggaug agggcgugga accggacgau      300

guggacuguu ggugcaauac cacaucuacc ugggucgugu augggacgug ucaucacaag      360

aaaggagagg cucggcgcag cagacgggcc guuacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggca gcucuacguc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuaca gcauucggug caucgggguu ucuaaccgcg auuuugugga aggaaugucc      660

ggcgggacau gggucgacgu ggugcuggag cauggaggcu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucagcaauau ggccgaagug      780

agaucuuacu gcuaugaggc uuccaucagc gauauggccu ccgacucucg gugucccacg      840

caaggggaag ccuaccugga caaacagagc gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gauggggcaa cggcuguggg cuguucggaa aaggcucccu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca gggaagucua uccagccuga gaaucucgaa     1020

uaccggauua ugcuguccgu gcacggaagc caacacucug gcaugaucgu uaacgauacc     1080

gggcaugaga cggacgagaa uagagccaaa guggaaauua caccgaacag cccccgcgcu     1140

gaggccaccc uuggaggcuu cggcucccug gggcucgauu gugaaccucg gaccggacug     1200

gacuuuucug aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggcgcug auaccgggac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggaa gccaggaggg cgccgugcau accgcccugg cuggggcccu ugaagcugag     1440

auggauggcg ccaagggacg ccuguccagc ggccaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggggu cucuuauucc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggaacggug accguugagg ugcaguacgc cggcacagac     1620

gggccuugca agguccccgc ucaaauggcc guggauaccc agacacugac cccuguggga     1680

cgccucauca cggcgaaccc cguuaucaca gaaagcaccg aaaauucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg cgacuccuac auugucaucg gggugggcga gaaaaagauu     1800

acacaccacu ggcaucggag cggauccacc aucggcaaag ccuuugaggc uaccgugaga     1860

ggggccaagc gcauggcugu ccugggagac acagccuggg auuucggcuc ugucggggga     1920

gcccucaaca gccugggcaa agggauucac cagaucuucg gcgcugcguu uaagucccug     1980

uucggaggca ugucuugguu uagccaaauc cuuauuggga cgcuccugau guggcuggga     2040

cucaauacca agaacggcuc caucagccug augugucuug cccucggggg aguucugauu     2100

uuccugucua cagcuguguc cgccgacguc ggcugcagcg uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8937
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 3 opt

<400>  8937
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccaaug uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggca gcgccuacua uauguaccuu gauagaaacg acgccgggga ggcuauuucc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugucuua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac cacaagcacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc cagacgggcc guuacccuuc ccucucauag cacacgcaag      420

cugcaaacca gaucccagac auggcucgag ucucgggaau acaccaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggga gcuccacgag ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cuauucggug caucggaguu uccaaucgcg auuuugugga aggcaugagc      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucucuaacau ggccgaagug      780

agauccuacu gcuaugaggc uagcaucucu gauauggccu ccgacagccg gugucccacg      840

caaggagaag ccuaccugga caaacagucc gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucucu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggaaagucca uccagccuga gaaccucgaa     1020

uaccggauua ugcugagcgu ccacggcucu caacacuccg ggaugaucgu uaaugauacc     1080

ggacaugaga cggacgagaa cagagccaaa guggaaauua caccgaauag cccccgcgcu     1140

gaggccaccc uuggcggcuu cgggucucug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuagcg aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aacaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cccaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaucc cguuaucacg gaaucuacag agaacuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc uggcuccaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagcccgcc ggauggcugu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcccucaauu cccuggggaa gggcauucac cagaucuucg gagcugcguu uaaaucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaacacga agaauggguc uaucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucca ccgcugugag cgccgacguc gggugcucug uggauuuuuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8938
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 3 opt

<400>  8938
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gaaaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugagguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gacagaaaug augccgggga agcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaccu cggccacaug      240

ugugaugcca caaugagcua cgagugcccu augcuggacg aaggggugga gccggaugac      300

guggauuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgaa agccgggagu acaccaagca ccugauccgc      480

guggaaaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg acuuugugga gggcaugucu      660

gggggaacau gggucgaugu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gacaagccua caguggauau ugaacuugug accaccacag ucuccaauau ggccgaggug      780

agaagcuacu gcuaugaggc uucuaucucc gacauggcca gcgauucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gacacccagu auguuugcaa gagaacacuc      900

guggaucgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga aaaucucgag     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgacacc     1080

ggacaugaga cggaugaaaa uagagccaaa guggagauua caccgaacuc uccccgcgcu     1140

gaagccaccc uuggcggcuu cgggagccug ggacucgacu gugagccucg gaccggccug     1200

gauuuuuccg accuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagaa     1260

ugguuccacg auaucccccu gccuuggcac gccggggcug acaccggaac accccauugg     1320

aauaacaagg aggcccuggu ggaguuuaaa gaugcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaagg ggccgugcau accgcccugg cuggcgcccu ugaggcugaa     1440

auggacggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggau     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug agacccugca uggcacggug accguugaag ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggacaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaguccacag agaauagcaa gaugaugcug     1740

gaacuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgau accgccuggg acuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgauguc gggugcuccg uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8939
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 3 opt

<400>  8939
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cccugcggug cauuggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuugag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau cgagcuggug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuauuucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccucga caaacagucu gauacccagu auguuugcaa gagaacacug      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu cgugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucuggaa     1020

uaccggauca ugcuuucugu ccacggcucc caacacagcg ggaugauugu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc ugggcggcuu cgggagccuc ggacuggauu gugaaccucg gaccggccuu     1200

gacuuuuccg aucuguauua ccucacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccucgu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucugggcu cucaggaggg ggccgugcau accgcccuug cuggcgcccu ggaagcugag     1440

auggauggag ccaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggac     1500

aaacugagac ucaagggagu cagcuauucu cuguguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccucac accugugggc     1680

cgccugauua ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggauc ccccuuucgg ggacucuuac aucgucauug gcgugggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccucggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccugaacu cucuggggaa aggcauccac cagauuuucg gagcugcguu uaagagccuu     1980

uucggcggga uguccugguu uucucaaauc cucaucggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag cauuucccuu augugucugg cccucggagg cguucugauc     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8940
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 3 opt

<400>  8940
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccaaug uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggca gcgccuacua uauguaccuu gauagaaacg acgccgggga ggcuauuucc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugucuua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac cacaagcacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc cagacgggcc guuacccuuc ccucucauag cacacgcaag      420

cugcaaacca gaucccagac auggcucgag ucucgggaau acaccaagca ccugauccgc      480

guggagaauu ggauguuuag aaacccuggc uucgcgcugg ccgcugccgc uaucgccugg      540

cuccugggga gcuccacgag ccagaaaguc auuuaucuug ugaugauccu gcucauugcg      600

cccgccuacu cuauccggug cauuggaguu uccaaucgcg auuuugugga aggcaugagc      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau cgagcuugug accaccacag ucucuaacau ggccgaagug      780

agauccuacu gcuaugaggc uagcauuucu gauauggccu ccgacagccg gugucccacg      840

caaggagaag ccuaccugga caaacagucc gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucucu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggaaagucca uccagccuga gaaccucgaa     1020

uaccggauca ugcugagcgu ccacggcucu caacacuccg ggaugauugu uaaugauacc     1080

ggacaugaga cggacgagaa cagagccaaa guggaaauca caccgaauag cccccgcgcu     1140

gaggccaccc uuggcggcuu cgggucucug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuagcg aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aacaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cccaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauua ccgcgaaucc cguuaucacg gaaucuacag agaacuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac aucgucauug gcgugggaga gaaaaagauc     1800

acccaccacu ggcaucgguc uggcuccaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcccucaauu cccuggggaa aggcauccac cagauuuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuaucggaa cacuccugau guggcugggc     2040

cucaacacga agaauggguc uauuagccug augugucuug cccugggagg cguucucauc     2100

uuccugucca ccgcugugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8941
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 3 opt

<400>  8941
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggauccugag aaauccuggc uuugcgcucg ccgcugccgc uauugccugg      540

cugcuugggu ccagcacguc ucagaaaguc aucuaucugg ugaugauucu ccugaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuucgugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuugag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuggug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccucga caaacagucu gauacccagu auguuugcaa gagaacacug      900

guggaccgcg gcuggggcaa cgggugugga cuguuuggca aagggagccu cgugaccugc      960

gcuaaguucg cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucuggaa     1020

uaccggauua ugcuuucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc ugggcggcuu ugggagccuc ggacuggauu gugaaccucg gaccggccuu     1200

gacuucuccg aucuguauua ccucacaaug aauaacaagc acuggcuggu ccauaaagag     1260

ugguuucacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccucgu ggaguucaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucugggcu cucaggaggg ggccgugcau accgcccuug cuggcgcccu ggaagcugag     1440

auggauggag ccaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggac     1500

aaacugagac ucaagggagu cagcuauucu cuguguacag cggccuuuac cuucacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccucac accugugggc     1680

cgccugauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggauc ccccuuuugg ggacucuuac auugucaucg gcauuggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuucgaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcucggcgac accgccuggg auuuuggguc cgucggaggc     1920

gcccugaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu caagagccuu     1980

uuuggcggga uguccugguu cucucaaauu cucaucggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag cauuucccuu augugucugg cccucggagg cgugcugauc     2100

uuucugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8942
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 3 opt

<400>  8942
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug uccaccaccg ucagcaauau ggccgaagug      780

agaucuuacu gcuaugaggc uacaaucucc gauaucgcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggccguc     1380

gugcucggcu cucaggaggg ggccguucau accgcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag ccgcuuucac cuuuacaaaa     1560

aucccggccg aaacccugca uggcacgguc accguggagg uucaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcg gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgccaaccc cgugauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaagg cuuuugaagc caccgugaga     1860

ggagcuaaac gcauggccgu gcugggcgac accgccuggg auuucggguc cgucggaggc     1920

gcucucaacu cucuggggaa aggcauccac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cucugggagg cgugcucauc     2100

uuccugagca ccgccguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8943
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 3 opt

<400>  8943
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggca cccaggaggg ggccgugcau acagcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacgcugca uggcaccgug acaguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cggcgaaccc cguuaucaca gaaucuaccg aaaauuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc uggcuccacc aucgggaaag ccuuugaggc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac acagccuggg auuucgggag cgugggaggc     1920

gcccucaacu cccuggggaa aggcauucac cagaucuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaauacca agaacggguc uaucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucca cagcugugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8944
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 3 opt

<400>  8944
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagc ugcaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccucac accugugggc     1680

cgccugauca ccgcgaaccc cgugaucacg gaauccacag agaauagcaa gaugaugcuu     1740

gagcuggauc ccccuuucgg ggacucuuac auuguuaucg gcgucggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcucggcgac accgccuggg auuucggguc cgucggaggc     1920

gcccugaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccuu     1980

uucggcggga uguccugguu uucucaaauc cucauuggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag caucucccuu augugucugg cccucggagg cgugcugauu     2100

uuccugagca ccgcuguuuc ugccgacgug gggugcuccg ucgauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8945
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 3 opt

<400>  8945
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gaaaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugagguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gacagaaaug augccgggga agcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaccu cggccacaug      240

ugugaugcca caaugagcua cgagugcccu augcuggacg aaggggugga gccggaugac      300

guggauuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgaa agccgggagu acaccaagca ccugauccgc      480

aucgaaaacu ggauuuuuag aaauccuggc uucgcgcugg ccgcugccgc uaucgccugg      540

cuccuggggu ccagcacguc ucagaaagug auuuaucuug ucaugauccu gcucauugcg      600

cccgccuacu ccauccggug cauuggagug agcaaccgcg acuuuguuga gggcaugucu      660

gggggaacau ggguggaugu cgugcuggag cauggcgggu gugugaccgu cauggcucag      720

gacaagccua caguugauau cgaacuugug accaccacag uguccaauau ggccgagguc      780

agaagcuacu gcuaugaggc uucuaucucc gacauggcca gcgauucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gacacccagu augugugcaa gagaacacuc      900

guugaucgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga aaaucucgag     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgacacc     1080

ggacaugaga cggaugaaaa uagagccaaa guugagauca caccgaacuc uccccgcgcu     1140

gaagccaccc uuggcggcuu cgggagccug ggacucgacu gugagccucg gaccggccug     1200

gauuuuuccg accuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagaa     1260

ugguuccacg auauuccccu gccuuggcac gccggggcug acaccggaac accccauugg     1320

aauaacaagg aggcccuggu cgaguuuaaa gaugcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cucaggaagg ggccguucau accgcccugg cuggcgcccu ugaggcugaa     1440

auggacggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggau     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug agacccugca uggcacgguc accguggaag uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgacaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cgugauuacg gaguccacag agaauagcaa gaugaugcug     1740

gaacuugauc ccccuuucgg ggacucuuac aucguuauug gcgucggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgau accgccuggg acuucggguc cgucggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cccugggagg cgugcucauc     2100

uuccugagca ccgcuguuuc ugccgaugug gggugcuccg ucgacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8946
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 3 opt

<400>  8946
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaauccauug agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucgucauug gcgugggaga gaaaaagauc     1800

acacaccacu ggcaucgguc cggcagcacc auugggaaag ccuuugaagc uacagugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cccuccugau guggcugggc     2040

cucaauacaa agaacgggag cauuucccug augugucuug cccugggagg cguucucauc     2100

uuccugagca cggcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8947
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 3 opt

<400>  8947
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ucaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag ccgcguucac cuuuacaaaa     1560

auuccggccg aaacccugca uggcacgguu accguggagg uccaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu guggauaugc agacccugac accuguuggc     1680

cgccucauca ccgccaaccc cgucaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugugaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag cguuugaagc caccgucaga     1860

ggagcuaagc gcauggccgu gcugggcgac accgcuuggg auuucggguc cguuggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagccgcuuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucuccuuc augugucugg cgcuuggagg cgugcugauu     2100

uuucucagca ccgccgucuc ugcugacgug gggugcuccg uugauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8948
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 3 opt

<400>  8948
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggcaa acugagcucc gggcaccuca agugccgccu gaaaauggac     1500

aaacuucggc ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaag     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

agacucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa aaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga aaagaaaauu     1800

acccaccacu ggcaucgcuc cggcagcaca aucgggaagg ccuuugaggc uaccgugcgg     1860

ggagccaaaa gaauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa gggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga aaaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8949
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 3 opt

<400>  8949
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gaggcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaagg ggccgugcau accgcccugg cuggcgcccu ugaggcugaa     1440

auggacggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggau     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug agacccugca uggcacggug accguugaag ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggacaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaguccacag agaauagcaa gaugaugcug     1740

gaacuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgau accgccuggg acuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgauguc gggugcuccg uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8950
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 3 opt

<400>  8950
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cggggcugac     1620

ggaccuugca agguccccgc ccaaauggcg guggauaugc agacacugac cccugugggc     1680

cgccucauca cagccaaccc cguuaucacc gaauccacgg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcucucaacu cucuggggaa aggcauucac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaauacaa agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca cggccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8951
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 3 opt

<400>  8951
gggagaaagc uuaccaugag aaucauuaac gccggcaagg agaaaaagcg gcgcggggcu       60

gacaccaaug ugggaaucgu cggccugcuc cugacaacgg ccauggcggc ugaaguuacc      120

agacggggga gcgccuacua uauguaccuu gaucgcaacg acgccggaga ggcuauuucc      180

uuccccacaa cccugggcau gaauaaaugc uauauccaga uuauggaucu cgggcacaug      240

ugugacgcca caaugucuua cgaaugcccu augcuggaug agggagugga accggacgau      300

guggacuguu ggugcaacac cacaagcacc ugggucgugu auggcacgug ucaucacaag      360

aaaggcgagg cuagacgguc ccgcagagcc guuacccuuc ccucucauag cacacggaag      420

cugcaaaccc gcucccagac auggcucgag ucuagagaau acaccaagca ccugauccgg      480

guggagaauu ggaucuuucg caacccuggg uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggaa gcuccacgag ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cuauuagaug caucggcguu uccaaucggg auuuugugga agggaugagc      660

ggaggcacau gggucgacgu ggugcuggag caugggggau gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucucuaacau ggccgaagug      780

cgcuccuacu gcuaugaggc uagcaucucu gauauggccu ccgacagcag augucccacg      840

caaggcgaag ccuaccugga caaacagucc gauacccagu auguuugcaa gcggacacuc      900

guggacagag gcugggggaa uggauguggc cuguucggga aaggaucucu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggcaagucca uccagccuga gaaccucgaa     1020

uaccgcauua ugcugagcgu ccacgggucu caacacuccg gaaugaucgu uaaugauacc     1080

ggccaugaga cggacgagaa ccgggccaaa guggaaauua caccgaauag ccccagagcu     1140

gaggccaccc uuggcggguu cggaucucug ggccucgauu gugaaccucg caccgggcug     1200

gacuuuagcg aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggagcug auaccggcac accccauugg     1320

aacaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagcggca gacggucgug     1380

guucucgggu cccaggaggg cgccgugcau accgcccugg cuggagcccu ugaagcugag     1440

auggauggcg ccaaggggag acugucuagc ggacaccuca aaugccgccu gaagauggac     1500

aaacuucggc ugaagggcgu cuccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca ugggacggug accguugagg ugcaguacgc cggaacagac     1620

ggcccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggg     1680

agacucauca ccgcgaaucc cguuaucacg gaaucuacag agaacuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg cgacagcuac auugucaucg gagugggcga gaaaaagauu     1800

acccaccacu ggcaucgcuc uggguccaca aucggaaaag ccuuugaagc uaccgugcgg     1860

ggcgccagac gcauggcugu ccugggggac accgccuggg auuucggaag cgugggcggg     1920

gcccucaauu cccugggcaa gggcauucac cagaucuucg gagcugcguu uaaaucucug     1980

uucgggggaa ugagcugguu uucccaaauc cuuauuggca cacuccugau guggcugggg     2040

cucaacacga agaauggauc uaucagccug augugucuug cccugggcgg gguucucauu     2100

uuccugucca ccgcugugag cgccgacguc ggcugcucug uggauuuuuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8952
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 3 opt

<400>  8952
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gaaaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugagguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gacagaaaag augccgggga agcuauuucu      180

uuccccacaa cccugggaau gaauaagugc uauauccaga uuauggaccu cggccacaug      240

ugugaugcca caaugagcua cgagugcccu augcuggacg aaggggugga gccggaugac      300

guggauuguu ggugcaacac cacauccacc ugggucgugu auggaacgug ucaucacaaa      360

aagggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgaa agccgggagu acaccaaaca ccugauccgc      480

guggaaaauu ggaucuuuag aaacccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaagguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaucgcg acuuugugga gggcaugucu      660

gggggaacau gggucgaugu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gacaaaccua caguggauau ugaacuugug accaccacag ucuccaacau ggccgaggug      780

agaagcuacu gcuaugaggc uucuaucucc gacauggcca gcgauucccg gugucccacg      840

caaggagaag ccuaccugga caagcagucu gacacccagu auguuugcaa aagaacacuc      900

guggaucgcg gcuggggcaa ugggugugga cuguucggca aggggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa aaagaugaca ggaaaaagca uccagccuga aaaccucgag     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaaugacacc     1080

ggacaugaga cggaugaaaa cagagccaag guggagauua cacccaauuc uccgcgcgcu     1140

gaagccaccc uuggcggcuu cgggagccug ggacucgacu gugagccucg gaccggccug     1200

gauuuuuccg accuuuauua ccugacaaug aacaauaaac acuggcucgu ccauaaggaa     1260

ugguuccacg auaucccccu gccuuggcac gccggggcug acaccggaac accccauugg     1320

aacaauaaag aggcccuggu ggaguuuaag gaugcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaagg ggccgugcau accgcccugg cuggcgcccu ugaggcugaa     1440

auggacggag ccaaaggccg ccugagcucc gggcaccuca agugccggcu gaaaauggau     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaag     1560

auuccggcug agacccugca uggcacggug accguugaag ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggacaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaguccacag agaacagcaa aaugaugcug     1740

gaacuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaagaaaauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaagg ccuuugaagc uaccgugaga     1860

ggagccaaac gcauggcugu ccugggcgau accgccuggg acuucggguc cgugggaggc     1920

gcccucaauu cucuggggaa gggcauucac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaacacga aaaaugggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgauguc gggugcuccg uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8953
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 3 opt

<400>  8953
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauaugguua gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

gucgaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gccaaguuug cuugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ucacggcucc caacacagcg ggaugaucgu gaacgauacc     1080

ggacaugaga cggacgagaa uagagcgaaa gucgaaauua caccgaacuc uccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auaccggaac accccauugg     1320

aauaacaagg aagcucuggu cgaguuuaaa gacgcccacg cgaagagaca gacggugguu     1380

gugcucggcu cucaggaggg ggcuguccau accgcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gagcuauucu cucuguacag ccgcguucac cuuuacaaaa     1560

auuccggccg aaacccugca uggcacgguu accguggagg uccaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu guggauaugc agacccugac accuguuggc     1680

cgccucauca ccgccaaccc cgucaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugugaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag cguuugaagc caccgucaga     1860

ggagcuaagc gcauggccgu gcugggcgac accgcuuggg auuucggguc cguuggaggc     1920

gcccucaacu cucuggggaa aggcauucac cagaucuucg gagccgcuuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cgcugggagg cgugcucauu     2100

uuccugagca ccgccgucuc ugcugacgug gggugcuccg uugauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8954
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 3 opt

<400>  8954
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccaaug uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggca gcgccuacua uauguaccuu gauagaaacg acgccgggga ggcuauuucc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugucuua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacau gaccagcaca ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc cagacgggcc guuacgcuuc ccucucauag cacccgcaag      420

cugcaaacaa gaucccagac cuggcucgag ucucgggaau acacaaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggga gcuccaccag ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu cuauucggug caucggaguu uccaaucgcg auuuugugga aggcaugagc      660

gggggaacgu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcucag      720

gauaagccua ccguggacau ugagcuugug acaaccaccg ucucuaacau ggccgaagug      780

agauccuacu gcuaugaggc uagcaucucu gauauggccu ccgacagccg gugucccaca      840

caaggagaag ccuaccugga caaacagucc gauacgcagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucucu ggucacaugc      960

gcuaaguuug cgugcagcaa gaaaaugacc ggaaagucca uccagccuga gaaccucgaa     1020

uaccggauua ugcugagcgu gcacggcucu caacacuccg ggaugaucgu uaaugauaca     1080

ggacaugaga ccgacgagaa cagagccaaa guggaaauua cgccgaauag cccccgcgcu     1140

gaggccacac uuggcggcuu cgggucucug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuagcg aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auacaggaac accccauugg     1320

aacaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gaccgucgug     1380

guucucggcu cccaggaggg ggccgugcau acggcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cuccuauagc cucuguaccg cggccuucac auuuaccaaa     1560

auuccggcug aaacacugca uggcaccgug acgguugagg ugcaguacgc cgggaccgac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1680

cgccucauca cagcgaaucc cguuaucacc gaaucuacgg agaacuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc uggcuccacc aucgggaaag ccuuugaagc uacagugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucgggag cgugggaggc     1920

gcccucaauu cccuggggaa aggcauucac cagaucuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaacacaa agaauggguc uaucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucca cggcugugag cgccgacguc gggugcucug uggauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8955
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 3 opt

<400>  8955
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagccuga gaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauua caccgaacuc uccccgcgcu     1140

gaggccgccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gcuggggccg auacaggaac accccauugg     1320

aauaacaagg aagcgcuggu ggaguuuaaa gacgcucacg ccaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acggcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg ccgcuuucac auuuaccaaa     1560

auuccggccg aaacacugca uggcaccgug acgguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcg guggauaugc agacacugac cccugugggc     1680

cgccucauca cagccaaccc cguuaucacc gaauccacgg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcucucaacu cucuggggaa aggcauucac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaacc cuuaucggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag cauuucccug augugucuug cucugggagg cguucucauc     2100

uuccugagca ccgccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8956
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 3 opt

<400>  8956
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gaaaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugagguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gacagaaaug augccgggga agcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaccu cggccacaug      240

ugugaugcca caaugagcua cgagugcccc augcuggacg aaggggugga gccggaugac      300

guggauuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgaa agccgggagu acaccaagca ccugauccgc      480

guggaaaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cuggccuacu ccauucggug caucggaguu agcaaccgcg acuuugugga gggcaugucu      660

gggggaacau gggucgaugu ggugcuugag cauggcgggu gugucaccgu uauggcucag      720

gacaagccca caguggauau ugaacuggug accaccacag ucuccaauau ggccgaggug      780

agaagcuacu gcuaugaggc uucuaucucc gacauggcca gcgauucccg guguccuacg      840

caaggagaag ccuaccucga caaacagucu gacacccagu auguuugcaa gagaacacug      900

guggaucgcg gcuggggcaa cgggugugga cuguucggca aagggagccu cgugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccagcccga aaaucuggag     1020

uaccggauua ugcuuucugu ccacggcucc caacacagcg ggaugaucgu uaacgacacc     1080

ggacaugaga cggaugaaaa uagagccaaa guggagauua caccuaacuc uccgcgcgcu     1140

gaagccaccc ugggcggcuu cgggagccuc ggacuggacu gugagccccg gaccggccuu     1200

gauuuuuccg accuguauua ccucacaaug aauaacaagc acuggcuggu ccauaaagaa     1260

ugguuccacg auaucccucu gcccuggcac gccggggcug acaccggaac accucauugg     1320

aauaacaagg aggcccucgu ggaguuuaaa gaugcgcacg cuaagagaca gacggucgug     1380

guucugggcu cucaggaagg ggccgugcau accgcccuug cuggcgcccu ggaggcugaa     1440

auggacggag ccaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggau     1500

aaacugagac ucaagggagu cagcuauucu cuguguacag cggccuucac cuuuacaaaa     1560

auucccgcug agacccugca uggcacggug accguugaag ugcaguacgc cgggacagac     1620

ggaccgugca aggucccugc ucaaauggcc guggacaugc agacccucac acccgugggc     1680

cgccugauca ccgcgaaccc uguuaucacg gaguccacag agaauagcaa gaugaugcuu     1740

gaacuggauc cucccuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcagcaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccucggcgau accgccuggg acuucggguc cgugggaggc     1920

gcccugaacu cucuggggaa aggcauucac cagaucuucg gagcugcguu uaagagccuu     1980

uucggcggga uguccugguu uucucaaauc cucauuggaa cacugcugau guggcucggc     2040

cugaauacga agaacgggag caucucccuu augugucugg cccucggagg cguucugauu     2100

uuccugagca ccgcuguguc ugccgauguc gggugcuccg uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8957
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 3 opt

<400>  8957
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaauu agcaaccgcg auuuuguuga aggcaugucu      660

gggggaacau ggguggacgu cgugcuggag cauggcgggu gugugaccgu cauggcucag      720

gauaagccua caguugacau cgagcuugug accaccacag uguccaauau ggccgaaguc      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guugaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggauucug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuagcg aucuuuauua ccugacaaug aauaacaagc acuggcucgu gcauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcgcacg cuaagagaca gacggugguc     1380

gugcucggcu cccaggaggg ggccguucau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugucuagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu guccuauagc cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacgguc accguggagg uucaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc gucgauaugc agacccugac accugugggc     1680

cgccucauua ccgcgaaccc cgugaucacg gaaucuacag aaaauuccaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacagcuac auuguuaucg gcgucggaga gaaaaagauu     1800

acccaccacu ggcaucgguc uggcuccaca aucgggaaag ccuuugaggc uaccgugaga     1860

ggagccaagc gcauggcugu gcugggcgac accgccuggg auuucgggag cgucggaggc     1920

gcccucaacu cccuggggaa aggcauccac cagauuuucg gagcugcguu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacggguc uaucagccug augugucuug cccugggagg cgugcucauu     2100

uuccugucca ccgcuguuag cgccgacgug gggugcucug ucgauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8958
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 3 opt

<400>  8958
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaca      240

ugugacgcca ccaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac aaccuccacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacacuuc ccagccauuc cauccgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggauuuuuag aaauccuggc uucgcgcugg ccgcugccgc uaucgccugg      540

cuccuggggu ccagcacguc ucagaaaguc auuuaucuug ugaugauccu gcucauugcg      600

cccgccuacu ccauccggug cauuggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau cgagcuugug accaccacag ucuccaauau ggccgaagug      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uucagccuga gaaucucgaa     1020

uaccggauca ugcugucugu ccacggcucc caacacagcg ggaugauugu uaacgauacc     1080

ggacaugaga cggacgagaa uagagccaaa guggaaauca caccgaacuc uccccgcgcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

aucccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuauuacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac aucgucauug gcgugggaga gaaaaagauc     1800

acccaccacu ggcaucgguc cggcagcaca auugggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauccac cagaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cuuaucggaa cacuccugau guggcugggc     2040

cucaaugcca agaacgggag cauuucccug augugucuug cucugggagg cguucucauc     2100

uuccugagca cggccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8959
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 3 opt

<400>  8959
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucaacgguc ucgcagagcc guuacccuuc ccagccauuc cacacggaag      420

cugcagaccc gcucucagac auggcucgag agcagagaau acaccaagca ccugauccgg      480

guggagaacu ggaucuuucg caauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauuagaug caucggaguu agcaaccggg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucaa      720

gauaagccua caguggacau ugagcuugug accaccacag ucuccaauau ggccgaagug      780

cgcagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacuccag augucccacg      840

cagggagaag ccuaccugga caaacagucu gauacccagu auguuugcaa gcggacacuc      900

guggacagag gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcuaaguuug cgugcuccaa gaaaaugaca ggaaagagca uccaaccuga gaaucucgaa     1020

uaccgcauua ugcugucugu ccacggcucc cagcacagcg ggaugaucgu uaacgauacc     1080

ggacaugaga cggacgagaa ucgggccaaa guggaaauua caccgaacuc ucccagagcu     1140

gaggccaccc uuggcggcuu cgggagccug ggacucgauu gugaaccucg caccggccug     1200

gacuuuuccg aucuuuauua ccugacaaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aauaacaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagcggca gacggucgug     1380

guucucggcu cucaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggcag acugagcucc gggcaccuca aaugccgccu gaagauggac     1500

aaacuucggc ugaagggagu cagcuauucu cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaauacgc cgggacagac     1620

ggaccuugca agguccccgc ucagauggcc guggauaugc agacccugac accugugggc     1680

agacucauca ccgcgaaccc cguuaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucgcuc cggcagcaca aucgggaaag ccuuugaagc uaccgugcgg     1860

ggagccaaga gaauggcugu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcccucaacu cucuggggaa aggcauucac caaaucuucg gagcugcguu uaagagccug     1980

uucggcggga uguccugguu uucucagauc cuuauuggaa cacuccugau guggcugggc     2040

cucaauacga agaacgggag caucucccug augugucuug cccugggagg cguucucauu     2100

uuccugagca ccgcuguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8960
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 3 opt

<400>  8960
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaagcga gaucucagac cuggcucgag agccgggaau acacaaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcccugg cugccgcugc cauugcgugg      540

cuccuggggu ccagcaccuc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcuuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacgu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug acaaccaccg ucuccaauau ggcugaagug      780

agaagcuacu gcuaugaggc cucuaucucc gauauggcca gcgacucccg gugucccaca      840

caaggagagg cuuaccugga caaacagucu gauacgcagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggucacaugc      960

gcgaaguuug ccugcuccaa gaaaaugacc ggaaagagca uccagccuga aaaucucgaa     1020

uaccggauua ugcugucugu gcacggcucc caacacagcg ggaugaucgu uaacgauaca     1080

ggacaugaga ccgacgagaa uagagcuaaa guggaaauua cgccgaacuc uccccgcgcc     1140

gaggccacac uuggcggcuu cgggagccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuuccg aucuuuauua ccugaccaug aauaacaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gcuggggccg auacaggaac accccauugg     1320

aauaacaagg aagcgcuggu ggaguuuaaa gacgcucacg ccaagagaca gaccgucgug     1380

guucucggcu cucaggaggg ggccgugcau acggcucugg ccggcgcucu ugaagccgag     1440

auggauggag cgaagggccg ccugagcucc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cagcuauucu cucuguaccg ccgcuuucac auuuaccaaa     1560

auuccggccg aaacacugca uggcaccgug acgguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcg guggauaugc agacacugac cccugugggc     1680

cgccucauca cagccaaccc cguuaucacc gaauccacgg agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacucuuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cuuuugaagc cacagugaga     1860

ggagcuaaac gcauggccgu ccugggcgac accgccuggg auuucggguc cgugggaggc     1920

gcucucaacu cucuggggaa aggcauucac cagaucuucg gagcggccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cuuauuggaa cccuccugau guggcugggc     2040

cucaauacaa agaacgggag caucucccug augugucuug cucugggagg cguucucauu     2100

uuccugagca cggccguguc ugccgacguc gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8961
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 3 opt

<400>  8961
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaucug acgccgggga ggcuauuagc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcag cagacgggcc guuacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggaau acaccaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccugggga gcucuacguc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgu uauggcucag      720

gauaagccua caguggacau ugagcuugug accaccacag ucagcaacau ggccgaagug      780

agaucuuacu gcuaugaggc uuccaucagc gauauggccu ccgacucucg gugucccacg      840

caaggagaag ccuaccugga caaacagagc gauacccagu auguuugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggugaccugc      960

gcuaaguuug cgugcagcaa gaaaaugaca ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu ccacggcagc caacacucug ggaugaucgu uaaugauacc     1080

ggacaugaga cggacgagaa cagagccaaa guggaaauua caccgaauag cccccgcgcu     1140

gaggccaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggcug auaccggaac accccauugg     1320

aacaauaagg aagcccuggu ggaguuuaaa gacgcgcacg cuaagagaca gacggucgug     1380

guucucggca gccaggaggg ggccgugcau accgcccugg cuggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguacag cggccuucac cuuuacaaaa     1560

auuccggcug aaacccugca uggcacggug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1680

cgccucauca ccgcgaaccc cguuaucacg gaaagcacag agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugucaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucggag cggcuccaca aucgggaaag ccuuugaagc uaccgugaga     1860

ggagccaagc gcauggcugu ccugggcgac accgccuggg auuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa aggcauucac cagaucuucg gagcugcguu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaacacga agaauggguc caucagccug augugucuug cccugggagg cguucucauu     2100

uuccugucua ccgcuguguc cgccgacguc gggugcagcg uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8962
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 3 opt

<400>  8962
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgc ggaaguuaca      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac aaccucuacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu guuacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacauc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggucacaugc      960

gccaaguuug cuugcagcaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu uaaugauacg     1080

ggacaugaga cagacgagaa cagagcgaaa guggaaauua ccccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac acaggggccg auacgggaac accccauugg     1320

aacaauaagg aagcccuggu ggaguuuaaa gacgcucacg ccaagagaca gaccgucgug     1380

guucucggca gccaggaggg ggcggugcau accgcucugg ccggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguacag cugccuucac cuuuacgaaa     1560

auuccggcgg aaacccugca uggcacagug accguugagg ugcaguacgc cgggacagac     1620

ggaccuugca agguccccgc ucaaauggcc guggauaugc agacccugac gccugugggc     1680

cgccucauca cagcuaaccc cguuaucacc gaagggacag agaacagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg cgacucuuac auugucaucg gagugggcga gaaaaagauu     1800

acccaccacu ggcaucgguc cgggagcacc aucggaaaag ccuuugaagc gacagugaga     1860

ggcgccaagc gcauggcugu ccugggggac acggccuggg auuucggauc cgugggcggg     1920

guucucaauu cucugggcaa aggcauucac cagaucuucg gagcugccuu uaagagccug     1980

uucgggggaa uguccugguu uucucaaauc cuuauuggca cccuccugau guggcugggg     2040

cucaacacaa agaauggaag caucucccug augugucuug cccugggcgg ggugcucauu     2100

uuccugagca ccgcugucuc ugcggacgug ggcugcuccg uugauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8963
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 3 opt

<400>  8963
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggc ugaaguuacc      120

cggcgcggcu ccgccuacua uauguaccuu gauagaaaug acgccgggga ggcuauuucu      180

uuccccacaa cccugggaau gaacaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca caaugagcua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaauac cacauccacc ugggucgugu auggaacgug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc guuacccuuc ccagccauuc cacacgcaag      420

cugcaaacca gaucucagac auggcucgag agccgggaau acaccaagca ccugauccgc      480

guggagaacu ggaucuuuag aaauccuggc uucgcgcugg ccgcugccgc uauugccugg      540

cuccuggggu ccagcacguc ucagaaaguc aucuaucuug ugaugauucu gcucaucgcg      600

cccgccuacu ccauucggug caucggaguu agcaaccgcg auuuugugga aggcaugucu      660

gggggaacau gggucgacgu ggugcuggag cauggcgggu gugucaccgc uauggcccag      720

gauaagccua caguugacau ugagcuugug accaccacag uguccaauau ggcugaaguc      780

agaagcuacu gcuaugaggc cucuaucucc gauauggcca gcgacucccg gugucccacg      840

caaggagagg cuuaccugga caaacagucu gauacccagu augugugcaa gagaacacuc      900

guugaccgcg gcuggggcaa cgggugugga cuguucggca aagggagccu ggugaccugc      960

gcgaaguuug ccugcuccaa gaaaaugaca ggaaagagca uccagccuga aaaucucgaa     1020

uaccggauua ugcugucugu ccacggcucc caacacagcg ggaugcuugu gaacgauacc     1080

ggacaugaga cggacgagaa uagagcuaaa guugaaauca caccgaacuc uccccgcgcc     1140

gaggccaccc ugggcggcuu cgggagccuc ggacuggauu gugaaccucg gaccggccuu     1200

gacuuuuccg aucuguauua ccucacaaug aauaacaagc acuggcuggc ucauaaagag     1260

ugguuccacg acauuccccu gccuuggcac gccggggcgg cuaccggaac accccauugg     1320

aauaacaagg aagcccucgu ggaguuuaaa gaugcccacg cuaagagaca gacggucgug     1380

guccugggcu cucaggaggg ggccgugcau accgcucuug ccggcgcgcu ggaagccgag     1440

auggacggag cuaagggccg ccucagcucc gggcaccuga aaugccggcu uaagauggau     1500

aaacugagac ucaagggagu uagcuauucu cuguguacag ccgcuuucac cuuuacaaaa     1560

aucccggccg aaaccgugga cggcacgguc accguggagg ggcaguacgg aggcacagac     1620

gggccuugca agguucccgc gcaaauggcc guggauaugc agacccugac accugucggc     1680

cgccucauua ccgcuaaccc cgugaucacg gaauccacag agaauagcaa gaugaugcug     1740

gagcuugauc ccccuuucgg agacucuuac aucgugauug gcguugggga gaaaaagauc     1800

acccaucacu ggcaccgguc cggaagcaca auuggcaaag ccuuugaagc uaccgucaga     1860

ggggccaagc gcauggccgu gcugggagac accgcuuggg auuucggcuc cgucgggggc     1920

gcgcucaacu cucugggaaa aggcauccau cagauuaucg gggccgcuuu caagagccug     1980

uuuggaggca uguccugguu cucucaaauc cuuauuggga cacuccugau guggcuggga     2040

cucaauacga agaacggcag caucucccug augugucuug cccucggggg cgugcugauu     2100

uuucugagca ccgccguguc uggaggcguu gggugcuccg uggacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8964
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 3 opt

<400>  8964
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgc ggaaguuaca      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac aaccucuacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu guuacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacauc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggucacaugc      960

gccaaguuug cuugcagcaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu uaaugauacg     1080

ggacaugaga cagacgagaa cagagcgaaa guggaaauua ccccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auacaggaac gccccauugg     1320

aacaauaagg aagcucuggu ggaguuuaaa gacgcccacg cgaagagaca gaccgucgug     1380

guucucggca gccaggaggg ggcugugcau acagcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguaccg ccgcguucac auuuagcaaa     1560

auuccggccg aaacccugca uggcacggug accgaggaag uucaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu gucgauaugc agacccugac accugugggc     1680

cgccucauca ccgccaaccc cgugaucacg gagucuacag agaacuccaa gaugaugcug     1740

gaacuugauc ccccuuucgg ggacagcuac auuguuaucg gcgucggaga gaaaaagauu     1800

acccaccacu ggcaucgguc cggcucuaca aucgggaaag cguuugaagc caccgugaga     1860

ggagcuaagc gcauggccgu gcugggcgac accgcuuggg auuucgggag cgucggaggc     1920

gcccucaauu cccuggggaa aggcauucac cagaucuucg gagccgcuuu uaagucucug     1980

uucggcggga ugagcugguu uucccaaauc cuuauuggaa cacuccugau guggcugggc     2040

cucaacacga agaaugggag caucucucug augugucuug cgcugggagg cgugcucauu     2100

uuccugucca ccgccguuag cgcugacgug gggugcucug ucgauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8965
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 3 opt

<400>  8965
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgc ggaaguuaca      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac aaccucuacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu guuacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacauc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggucacaugc      960

gccaaguuug cuugcagcaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu uaaugauacg     1080

ggacaugaga cagacgagaa cagagcgaaa guggaaauua ccccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auacaggaac gccccauugg     1320

aacaauaagg aagcucuggu ggaguuuaaa gacgcccacg cgaagagaca gaccgucgug     1380

guucucggca gccaggaggg ggcugugcau acagcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguaccg ccgcguucac auuuaccaaa     1560

auuccggccg aaacgcugca uggcaccgug acaguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcu guggauaugc agacacugac cccugugggc     1680

cgccucauca cggccaaccc cguuaucaca gaaagcaccg agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucggag cggcuccacc aucgggaaag cguuugaagc caccgugaga     1860

ggagcuaagc gcauggccgu ccugggcgac acagcuuggg auuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa aggcauucac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaacacca agaauggguc caucagccug augugucuug cgcugggagg cguucucauu     2100

uuccugucua cagccguguc cgcugacguc gggugcagcg uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8966
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 3 opt

<400>  8966
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgu ugaagugaca      120

cggcgcggca augcguacua uauguaccuu gauagauccg acgcugggga ggccauuucu      180

uuccccacca caaugggaau gaacaaaugc uauauccaga uuauggaucu gggccacaug      240

ugugacgcca ccaugagcua cgaaugcccu augcucgaug agggggugga accggacgau      300

gucgacuguu ggugcaauac aaccuccacg ugggugguuu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcuc uagacgggcc gugacacugc ccagccauuc cacccgcaaa      420

cuucaaacaa gaucucagac cuggcuggag agccgggaau acacgaagca ccucauccgc      480

gucgagaacu ggaucuuuag aaauccuggc uucgcucugg ccgcggccgc uauugccugg      540

cugcucgggu ccagcacauc ucagaaagug aucuaucugg uuaugauucu ucugaucgcu      600

cccgccuacu ccauucggug caucggagug agcaaccgcg auuuugucga aggcaugucu      660

gggggaaccu ggguggacgu gguccucgag cauggcgggu guguuacagu gauggcgcag      720

gauaagccua ccguggacau ugagcugguc accacaacgg uguccaauau ggccgaaguu      780

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacc      840

caaggagagg cuuaccuuga caaacagucu gauacacagu augugugcaa gagaacccug      900

gucgaccgcg gcuggggcaa cgggugugga cucuucggca aagggagccu ggugacaugc      960

gccaaguuug ccugcuccaa gaaaaugacc ggaaagagca uccagccuga aaaucuggaa     1020

uaccggauua ugcucucugu ucacggcucc caacacagcg ggaugaucgu gaacgauacg     1080

ggacaugaga cagacgagaa uagagcuaaa gucgaaauua ccccgaacuc uccccgcgcg     1140

gaggccaccc ugggcggcuu cgggagccuu ggacuggauu gugaaccucg gacaggccuc     1200

gacuuuuccg aucuguauua ccuuaccaug aauaacaagc acuggcuggu gcauaaagag     1260

ugguuccacg acaucccccu cccuuggcac gcuggggccg auacaggaac gccccauugg     1320

aauaacaagg aagcccuggu cgaguuuaaa gacgcucacg ccaagagaca gaccgugguu     1380

gugcugggcu cucaggaggg ggcgguccau acagcucucg ccggcgcccu ggaagcugag     1440

auggauggag ccaagggccg ccuuagcucc gggcaccuga aaugccggcu caagauggac     1500

aaacugagac uuaagggagu gagcuauucu cuguguaccg cugccuucac auuuaccaaa     1560

auuccggcgg aaacgcucca uggcaccguu acaguggagg uccaguacgc cgggaccgac     1620

ggaccuugca aggugcccgc ucaaauggcc guggauaugc agacacugac cccuguuggc     1680

cgccugauca cggcuaaccc cgucaucaca gaauccaccg agaauagcaa gaugaugcuc     1740

gagcuggauc ccccuuucgg ggacucuuac auugugaucg gcgugggaga aaagaagauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaaag ccuuugaggc gaccgucaga     1860

ggagccaagc gcauggcugu gcuuggcgac acagccuggg auuucggguc cguuggaggc     1920

gcucugaacu cucucgggaa aggcauucac cagaucuucg gagccgccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cgcuucucgu guggcugggc     2040

cugaauacca agaacgggag caucucccuc augugucugg cucuuggagg cguccugauu     2100

uuccucagca cagcgguguc ugccgacguu gggugcuccg uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8967
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 3 opt

<400>  8967
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgc ggaaguuaca      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

guggacuguu ggugcaacac aaccucuacg ugggucgugu auggaaccug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu guuacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacauc ccagaaaguc aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguu ucuaaucgcg auuuugugga aggcaugucc      660

gggggaaccu gggucgacgu ggugcuggag cauggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau ugagcuugug accacaacgg ucagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacc      840

caaggagaag ccuaccugga caaacagagc gauacacagu auguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggucacaugc      960

gccaaguuug cuugcagcaa gaaaaugacc ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu uaaugauacg     1080

ggacaugaga cagacgagaa cagagcgaaa guggaaauua ccccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gacaggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auacaggaac gccccauugg     1320

aacaauaagg aagcucuggu ggaguuuaaa gacgcccacg cgaagagaca gaccgucgug     1380

guucucggca gccaggaggg ggcugugcau acagcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu cucuuauucc cucuguaccg ccgcguucac auuuaccaaa     1560

auuccggccg aaacgcugca uggcaccgug acaguugagg ugcaguacgc ugggaccgac     1620

ggaccuugca agguccccgc ccaaauggcu guggauaugc agacacugac cccugugggc     1680

cgccucauca cggccaaccc cguuaucaca gaaagcaccg agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugucaucg gcgugggaga gaaaaagauu     1800

acacaccacu ggcaucggag cggcuccacc aucgggaaag cguuugaagc caccgugaga     1860

ggagcuaagc gcauggccgu ccugggcgac acagcuuggg auuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa aggcauucac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cgcuccugau guggcugggc     2040

cucaacacca agaauggguc caucagccug guuugucuug cgcugggagg cgugcucauu     2100

uuccugucua cagccgucuc cgcugacgug gggugcagcg uugauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8968
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 3 opt

<400>  8968
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcacc       60

gacacaagcg uggggaucgu cggacugcuc cugacgaccg cuauggccgu ugaagugaca      120

cggcgcggcu ccgcguacua uauguaccuu gauagaucug acgcugggga ggccauuagc      180

uuccccacca cacugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcca ccauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

gucgacuguu ggugcaacac aaccucuacg ugggugguuu auggaaccug ucaucacaag      360

aaaggcgagg cucggcgcag cagacgggcc gugacacuuc ccucccauuc uacccgcaag      420

cugcaaacaa gaagccagac cuggcucgag ucccgggaau acacgaagca ccugauccgc      480

gucgagaauu ggaucuuuag aaacccuggc uucgcucugg ccgcggccgc uauugccugg      540

cuccugggga gcucuacauc ccagaaagug aucuaucuug uuaugauucu gcucaucgcu      600

cccgccuaca gcauucggug caucggagug ucuaaucgcg auuuugucga aggcaugucc      660

gggggaaccu ggguggacgu gguccuggag cauggcgggu guguuacagu gauggcgcag      720

gauaagccug ccguggacau ugagcuuguc accaccacag ugagcaacau ggcugaaguu      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacg      840

caaggagaag ccuaccugga caaacagagc gauacccagu augugugcaa gagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu ggugaccugc      960

gccaaguuug cuugcagcaa gaaaaugaca ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu ucacggcagc caacacucug ggaugaucgu gaaugauacc     1080

ggacaugaga cggacgagaa cagagcgaaa gucgaaauua caccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugacaaug aacaauaagc acuggcucgu gcauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auaccggaac accccauugg     1320

aacaauaagg aagcucuggu cgaguuuaaa gacgcccacg cgaagagaca gacggugguu     1380

gugcucggca gccaggaggg ggcuguccau accgcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu gucuuauucc cucuguacag ccgcguucac cuuuacaaaa     1560

auuccggccg aaacccugca uggcacgguu accguggagg uccaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu guggauaugc agacccugac accuguuggc     1680

cgccucauca ccgccaaccc cgucaucacg gaaagcacag agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugugaucg gcgugggaga gaaaaagauu     1800

acccaccacu ggcaucggag cggcuccaca aucgggaaag cguuugaagc caccgucaga     1860

ggagcuaagc gcauggccgu gcugggcgac accgcuuggg auuucggguc uguuggaggc     1920

gcccucaaua gccuggggaa aggcauucac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cacuccuggu guggcugggc     2040

cucaacacga agaauggguc caucagccug accugucuug cgcugggagg cguccucauu     2100

uuccugucua cagccguguc cgcugacguu gggugcagcg uggauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8969
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 3 opt

<400>  8969
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggu ugaagugacc      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacaa cccugggaau gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua caauguccua cgaaugcccu augcuggaug agggggugga accggacgau      300

gucgacuguu ggugcaacac cacaucuacc ugggugguuu auggaacgug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu gugacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggaau acaccaagca ccugauccgc      480

gucgagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugu gauugccugg      540

cuccugggga gcucuacguc ccagaaaguu aucuaucuug ugaugauucu gcucaucgcu      600

cccgccuaca gcauucggug caucggaguc ucuaaucgcg auuuugugga aggcaugucc      660

gggggaacau ggguggacgu cguucuggag cauggcgggu gugugaccgu gauggcgcag      720

gauaagccua cagucgacau ugagcuugug accaccacag uuagcaacau ggccgaagug      780

agaucuuacu gcuaugaggc uuccaucagc gauauggccu ccgacucucg gugucccacg      840

caaggagagg cuuaccugga caaacagagc gauacccagu augucugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu gguuaccugc      960

gccaaguuug ccugcagcaa gaaaaugaca ggaaagucua uccagccuga aaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu caaugauacc     1080

ggacaugaga cggacgagaa cagagcuaaa guggaaauua caccgaauag cccccgcgcg     1140

gaggccaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gcuggggccg auaccggaac accccauugg     1320

aacaauaagg aagcccuggu ggaguuuaaa gacgcucacg ccaagagaca gacgguugug     1380

guccucggca gccaggaggg ggcggugcau accgcucugg ccggcgcccu ugaagcugag     1440

auggauggag ccaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu uucuuauucc cucuguacag cugccuucac cuuuacaaaa     1560

auuccggcgg aaacccugca uggcacggug accgucgagg ugcaguacgc cgggacagac     1620

ggaccuugca aggugcccgc ucaaauggcc guugauaugc agacccugac accugucggc     1680

cgccucauca ccgcuaaccc cgugaucacg gaaagcacag agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugugaucg gcgucggaga gaaaaagauu     1800

acccaccacu ggcaucggag cggcuccaca aucgggaaag ccuuugaagc gaccgugaga     1860

ggagccaagc gcauggcugu ucugggcgac accgccuggg auuucggguc ugugggaggc     1920

gcucucaaua gccuggggaa aggcauucac cagaucuucg gagccgccuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cacuccuggu cuggcugggc     2040

cucaacacga agaauggguc caucagccug accugucuug cucugggagg cgugcucauu     2100

uuccugucua cagcgguuuc cgccgacgug gggugcagcg ucgauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8970
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 3 opt

<400>  8970
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggu ugaagugacc      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggga ggccauuagc      180

uuccccacaa cccugggagu gaauaaaugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua caauguccua cgaaugcccu augcuggaug agggggucga accggacgau      300

guggacuguu ggugcaacac cacaucuacc uggguugugu auggaacgug ucaucacaag      360

aaaggcgagg cccggcgcag cagacgggcu gucacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggaau acaccaagca ccugauccgc      480

guggagaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacguc ccagaaaguu aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguc ucuaaucgcg auuuugugga aggcaugucc      660

gggggaacau ggguggacgu cguucuggag cauggcgggu gugugaccgu gauggcccag      720

gauaagccua cagucgacau ugagcuugug accaccacag uuagcaacau ggcugaagug      780

agaucuuacu gcuaugaggc cuccaucagc gauauggcuu ccgacucucg gugucccacg      840

caaggagaag ccuaccugga caaacagagc gauacccagu augucugcaa gagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aagggucccu gguuaccugc      960

gccaaguuug cuugcagcaa gaaaaugaca ggaaagucua uccagccuga gaaccucgaa     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu caaugauacc     1080

ggacaugaga cggacgagaa cagagcgaaa guggaaauua caccgaauag cccccgcgcc     1140

gaggcuaccc uuggcggcuu cgggucccug ggacucgauu gugaaccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugacaaug aacaauaagc acuggcucgu ccauaaagag     1260

ugguuccacg acaucccccu gccuuggcac gccggggccg auaccggaac accccauugg     1320

aacaauaagg aagcucuggu ggaguuuaaa gacgcccacg cgaagagaca gacgguugug     1380

guccucggca gccaggaggg ggcugugcau accgcccugg ccggcgcucu ugaagccgag     1440

auggauggag cuaagggccg ccuguccagc gggcaccuca aaugccggcu gaagauggac     1500

aaacuuagac ugaagggagu uucuuauucc cucuguacag ccgcguucac cuuuacaaaa     1560

auuccggccg aaacccugca uggcacggug accgucgagg ugcaguacgc ugggacagac     1620

ggaccuugca aggugcccgc ccaaauggcu guugauaugc agacccugac accugucggc     1680

cgccucauca ccgccaaccc cgugaucacg gaaagcacag agaacucuaa gaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac auugugaucg gcgucggaga caaaaagauu     1800

acccaccacu gguaucggag cggcuccaca aucgggaaag cguuugaagc caccgugaga     1860

ggagcuaagc gcauggccgu ucugggcgau accgcuuggg acuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa aggcauucau cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauc cuuauuggaa cacuccuggu cuggcugggc     2040

cucaacacga agaauggguc caucagccug accugucuug cgcugggagg cgugcucauu     2100

uuccugucua cagccguuuc cgcugaugug gggugcagcg ucgacuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8971
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 3 opt

<400>  8971
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agaaaaagcg cagaggcgcu       60

gacaccagcg uggggaucgu cggacugcuc cugacaacgg ccauggcggu ugaagugacc      120

cggcgcggcu ccgcuuacua uauguaccuu gauagaucug acgccgggaa agccauuagc      180

uuccccacaa cccugggagu gaauaagugc uauauccaga uuauggaucu cggccacaug      240

ugugacgcua caauguccua cgagugcccu augcuggaug aaggggucga gccggacgau      300

guggacuguu ggugcaacac cacaucuacc uggguugugu auggaacgug ucaucacaaa      360

aagggcgaag cccggcgcag cagacgggcu gucacccuuc ccucccauuc uacacgcaag      420

cugcaaacca gaagccagac auggcucgag ucccgggagu acaccaaaca ccugauccgc      480

guggaaaauu ggaucuuuag aaacccuggc uucgcccugg cggccgcugc cauugcuugg      540

cuccugggga gcucuacguc ccagaagguu aucuaucuug ugaugauucu gcucaucgcc      600

cccgcguaca gcauucggug caucggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaacau ggguggacgu cguucuggaa cauggcgggu gugugaccgu gauggcccag      720

gauaaaccua cagucgacau ugagcuugug accaccacag uuagcaacau ggcugaggug      780

agaucuuacu gcuaugaagc cuccaucagc gauauggcuu ccgacucucg gugucccacg      840

caaggagagg ccuaccugga caagcagagc gauacccagu augucugcaa aagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuaccugc      960

gccaaguuug cuugcagcaa aaagaugaca ggaaaaucua uccagccuga aaaccucgag     1020

uaccggauua ugcuguccgu gcacggcagc caacacucug ggaugaucgu caaugauauu     1080

ggacaugaaa ccgacgagaa cagagcgaag guggagauca cgccgaauag cccccgcgcc     1140

gaagcuacac uuggcggcuu cgggucccug ggacucgauu gugagccucg gaccggccug     1200

gacuuuucug aucuuuauua ccugaccaug aacaauaaac acuggcucgu ccauaaggaa     1260

ugguuccacg acauuccccu gccuuggcac gccggggccg auacaggaac accccauugg     1320

aacaauaaag aggcucuggu ggaauuuaag gacgcccacg cgaagagaca gacaguugug     1380

guccucggca gccaggaggg ggcugugcau acggcccugg ccggcgcucu ugaggccgaa     1440

auggauggag cuaaaggccg ccuguccagc gggcaccuca agugccggcu gaaaauggac     1500

aaacuuagac ugaagggagu uucuuauucc cucuguaccg ccgcguucac auuuaccaag     1560

aucccggccg agacacugca uggcaccgug acggucgaag ugcaguacgc ugggaccgac     1620

ggaccuugca aggugcccgc ccaaauggcu guugauaugc agacacugac cccugucggc     1680

cgccucauca cagccaaccc cgugauuacc gagagcacgg aaaacucuaa aaugaugcug     1740

gagcuugauc ccccuuucgg ggacuccuac aucgugauug gcgucggaga caagaaaauc     1800

acacaccacu ggcaucggag cggcuccacc auugggaagg cguuugaggc cacagugaga     1860

ggagcuaaac gcauggccgu ucugggcgau accgcuuggg acuucggguc ugugggaggc     1920

gcccucaaua gccuggggaa gggcauccac cagaucuucg gagccgcuuu uaagucccug     1980

uucggcggga ugucuugguu uagccaaauu cuuaucggaa cccuccuggu cuggcugggc     2040

cucaacacca aaaauggguc cauuagccug acgugucuug cgcugggagg cgugcucauc     2100

uuccugucua ccgccguuuc cgcugaugug gggugcagcg ucgacuuuuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8972
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 3 opt

<400>  8972
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucacgcugg ucgccguggc uaucgccugg      540

cuccugggga gcucuacauc ccagaagguu auuuaccuug ugaugauccu gcucauugcu      600

ccugccuaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaaccu ggguggacgu cguucuggaa cacggcgggu gugugacagu gauggcgcag      720

gauaaaccca ccgucgacau cgagcuugug accacaacgg uuagcaacau ggccgaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacc      840

caaggagagg cuuaucugga caagcagagc gauacacagu acgucugcaa aagaacccuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuacaugc      960

gccaaguuua ccugcagcaa aaagaugacg ggaaaaucua uucagcccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc caacauucug ggaugauugu caaugauaca     1080

ggacacgaaa ccgacgagaa cagagccaag guggagguca ccccuaauag cccgcgcgcu     1140

gaagcgacac uuggcggcuu cgggucccug ggacucgauu gugagccccg gaccggccug     1200

gacuuuucug aucuuuacua ucugacaaug aacaauaaac auuggcucgu gcacaaggaa     1260

ugguuccacg acaucccucu gcccuggcau gccggggcug auacgggaac cccucacugg     1320

aacaauaaag aggcccuggu ugaauuuaag gacgcccaug cuaagagaca gacagugguc     1380

gugcucggca gccaggaggg ggccguucac accgcgcugg cuggcgcccu ugaggccgaa     1440

auggauggag cuaaaggccg ccuguucucc gggcaucuca agugccggcu gaaaauggac     1500

aaacuuagac ugaaaggagu gagcuacucu cucuguacag ccgcuuuuac cuucacgaag     1560

guccccgccg agacccugca cggcacagug accguggaag uucaguaugc ggggacagac     1620

ggaccgugca aggucccugc ccaaauggcu guggauaugc agacccugac gcccgugggc     1680

cgccucauua cagccaaccc ugucaucacc gaguccacag aaaacagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgugauug gcguuggaga caagaaaauc     1800

acccaccauu ggcaccgguc cggcagcacc auugggaagg cuuucgaggc cacagugaga     1860

ggagcgaaac gcauggccgu ccugggcgau acggcuuggg acuucggguc cgugggaggc     1920

guuuuuaauu cucucgggaa gggcauccau cagauuuucg gagccgcuuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cugaucggaa cccuucucgu guggcugggc     2040

cugaacacaa agaaugggag cauuucccuc accugucugg cccuuggagg cgucaugauc     2100

uuccugagca cagccguguc ugcugauguu gggugcuccg uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8973
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 3 opt

<400>  8973
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa aucugggagu gaacaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua ccauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaauac aaccucuaca uggguggucu acggaaccug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacgcuuc cgucccacuc uaccagaaag      420

cugcaaacac ggagccagac cuggcucgag ucccgcgagu auacaaagca ucugauuaaa      480

guugaaaacu ggaucuuuag aaaucccggc uucgcccugg cugccguggc uauugccugg      540

cuccugggga gcucuaccuc ccagaagguc aucuaccuug ugaugauucu gcucaucgcg      600

ccugccuaua gcauucggug caucggaguu ucuaaccgcg auuuugugga gggcaugucc      660

gggggaacgu gggucgacgu ggugcuggaa cacggcgggu gugucacagu uauggcucag      720

gauaaaccca ccguggacau cgagcuugug acaaccaccg ucagcaauau ggccgaggug      780

agaucuuacu gcuacgaggc uuccauuagc gauauggccu ccgacucucg guguccuaca      840

caaggagaag ccuaucugga caagcagagc gauacgcagu acguuugcaa aagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aggggucccu ggucacaugc      960

gcuaaguuua ccugcagcaa aaagaugaca ggaaaaucua uccagcccga aaaucucgag     1020

uaucggauua ugcuguccgu gcacggcagc caacauucug ggaugaucgu uaacgauauu     1080

ggacacgaaa ccgacgagaa uagagcgaag guggagguca cgccuaacag cccgcgcgcc     1140

gaagcuacac uuggcggcuu cgggucccug ggacucgauu gugagccccg gaccggccug     1200

gacuuuucug aucuuuacua ucugaccaug aauaacaaac auuggcucgu gcacaaggaa     1260

ugguuccacg acaucccucu gcccuggcau gccggggccg auacaggaac cccucacugg     1320

aauaacaaag aggcucuggu cgaauuuaag gacgcccaug cgaagagaca gacagugguu     1380

gugcucggca gccaggaggg ggcuguccac acggcccugg ccggcgcucu ugaggccgaa     1440

auggauggag cuaaaggccg ccuguucucc gggcaucuca agugccggcu gaaaauggac     1500

aaacuuagac ugaaaggagu gagcuacucu cucuguaccg ccgcguuuac auucaccaag     1560

guucccgccg agacacugca cggcaccgug acggucgaag ugcaguaugc ugggaccgac     1620

ggaccgugca aggugccugc ccaaauggcu guugauaugc agacacugac ccccgucggc     1680

cgccucauca cagccaaccc ugugauuacc gaguccacgg aaaauagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgugauug gcgucggaga caagaaaauc     1800

acacaccauu ggcaccgguc cggcagcacc auugggaagg cguucgaggc cacagugaga     1860

ggagcuaaac gcauggccgu ucugggcgau accgcuuggg acuucggguc cgugggaggc     1920

gucuuuaacu cucucgggaa gggcgugcau cagaucuucg gagccgccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cugauuggaa cccuucucgu uuggcugggc     2040

cugaauacaa agaacgggag caucucccuc acgugucugg cucuuggagg cgugaugauu     2100

uuccugagca ccgcggucuc ugccgaugug gggugcuccg uugacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8974
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 3 opt

<400>  8974
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugauuaaa      480

guugaaaauu ggaucuuuag aaaccccggc uucgcccugg uggcugucgc cauugcuugg      540

cuccugggga gcucuacguc ccagaaggug aucuaccuug uuaugauucu gcucaucgcc      600

ccugcguaua gcauucggug caucggagug ucuaaucgcg auuuugucga gggcaugucc      660

gggggaacau ggguggacgu gguccuggaa cacggcgggu guguuaccgu gauggcccag      720

gauaaaccca caguggacau cgagcuuguc accaccacag ugagcaacau ggcugagguu      780

agaucuuacu gcuacgaagc cuccauuagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgugugcaa aagaacacuc      900

gucgaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu ggugaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uccagcccga aaaccucgag     1020

uaucggauua ugcuguccgu ucacggcagc caacauucug ggaugaucgu gaaugauauu     1080

ggacacgaaa cggacgagaa cagagcuaag gucgagguga caccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gaccggccug     1200

gacuuuucug aucuuuacua ucugacaaug aacaauaaac auuggcucgu ccacaaggaa     1260

ugguuccacg acaucccucu gcccuggcau gcuggggccg auaccggaac accucacugg     1320

aacaauaaag aggcccuggu ggaauuuaag gacgcucaug ccaagagaca gacgguugug     1380

guccucggca gccaggaggg ggcggugcac accgcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggccg ccuguucucc gggcaucuca agugccggcu gaaaauggac     1500

aaacuuagac ugaagggagu uagcuacucu cucuguacag cugccuuuac cuucacaaag     1560

gugcccgcgg agacccugca cggcacgguc accguggaag ugcaguaugc cgggacagac     1620

ggaccgugca agguuccugc ucaaauggcc gucgauaugc agacccugac acccgugggc     1680

cgccucauca ccgcuaaccc ugugauuacg gaguccacag aaaacagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgucauug gcgugggaga caagaaaauc     1800

acccaccauu ggcaccgguc cggcagcaca auugggaagg ccuucgaggc gaccguuaga     1860

ggagccaaac gcauggcugu gcugggcgau accgccuggg acuucggguc cgucggaggc     1920

guguuuaauu cucucgggaa gggcauccau cagaucuucg gagcugccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauu cugaucggaa cacuucucgu uuggcugggc     2040

cugaacacga aaaaugggag cauuucccuc accugucugg cccuuggagg cgugaugauc     2100

uuccugagca cagcugucuc ugcggaugug gggugcuccg uugacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8975
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 3 opt

<400>  8975
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa aucugggagu gaacaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua ccauguccua ugagugcccc augcuggaug aagggguuga gcccgacgau      300

guggacuguu ggugcaauac aaccucuaca uggguggucu acggaaccug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg gugacgcuuc cgucccacuc uaccagaaag      420

cugcaaacac ggagccagac cuggcucgag ucccgcgagu auacaaagca ucugauuaaa      480

guugaaaacu ggaucuuuag aaaucccggc uucgcccugg cugccguggc uauugccugg      540

cuccugggga gcucuaccuc ccagaagguc aucuaccuug ugaugauucu gcucaucgcg      600

ccugccuaua gcauucggug caucggaguu ucuaaccgcg auuuugugga gggcaugucc      660

gggggaacgu gggucgacgu ggugcuggaa cacggcgggu gugucacagu uauggcucag      720

gauaaaccca ccguggacau cgagcuugug acaaccaccg ucagcaauau ggccgaggug      780

agaucuuacu gcuacgaggc uuccauuagc gauauggccu ccgacucucg guguccuaca      840

caaggagaag ccuaucugga caagcagagc gauacgcagu acguuugcaa aagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguucggca aggggucccu ggucacaugc      960

gcuaaguuua ccugcagcaa aaagaugaca ggaaaaucua uccagcccga aaaucucgag     1020

uaucggauua ugcuguccgu gcacggcagc caacauucug ggaugaucgu uaacgaugaa     1080

aauagagcga agguggaggu caccccuaac agcccgcgcg ccgaggcuac gcuuggaggc     1140

uucggcuccc uggggcucga cugugaaccc cggacaggac uggauuuuuc ugaccuuuac     1200

uaucugacca ugaauaacaa acacuggcuc gugcauaagg agugguucca cgauauuccu     1260

cugcccuggc acgccggcgc cgacaccggg acaccucauu ggaauaacaa agaagcucug     1320

gucgaguuua aggaugccca cgcgaagaga cagaccgugg uugugcucgg aagccaggaa     1380

ggcgcugucc auacagcccu ggccggggcu cuugaggccg agauggacgg cgcuaaagga     1440

cgccuguucu ccggccaccu caagugccgg cugaaaaugg auaaacuuag acugaagggg     1500

gugagcuacu cucucuguac ggccgcguuu accuucacaa agguucccgc cgaaacccug     1560

cauggaacag ugaccgucga ggugcaguau gcuggcacgg acgggccgug caaggugccu     1620

gcccaaaugg cuguugacau gcagacccug acacccgucg gacgccucau caccgccaac     1680

ccugugauca cagaauccac cgagaauagc aaaaugaugc uggaacuuga uccucccuuu     1740

ggcgacucuu acauugugau cggggucggc gauaagaaaa uuacgcacca cuggcaucgg     1800

uccggaagca caaucggcaa ggcguucgag gccaccguga gaggggcuaa acgcauggcc     1860

guucugggag acacagcuug ggauuucggc uccgucgggg gaguguuuaa cucucucggc     1920

aaggggauuc accagaucuu cggcgccgcc uuuaagagcc uguucggagg cauguccugg     1980

uuuucucaaa uccugauugg gacccuucuc guguggcugg gacugaauac caaaaacggc     2040

agcaucuccc ucacaugucu ggcucuuggg ggaguuauga uuuuccugag cacggcggug     2100

ucugccgacg ucggcugcuc cguggauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8976
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 3 opt

<400>  8976
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucgcccugg ucgcuguggc caucgcuugg      540

cuccugggga gcucuacguc ccagaagguu auuuaccuug ugaugauccu gcucauugcc      600

ccugcguaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaacau ggguggacgu cguucuggaa cacggcgggu gugugaccgu gauggcccag      720

gauaaaccca cagucgacau cgagcuugug accaccacag uuagcaacau ggcugaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgucugcaa aagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uucagcccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc caacauucug ggaugauugu caaugauacg     1080

ggacacgaaa cagacgagaa cagagcuaag guggagguca ccccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gacaggccug     1200

gacuuuucug aucuuuacua ucugaccaug aacaauaaac auuggcucgu gcacaaggaa     1260

ugguuccacg acaucccucu gcccuggcau gcuggggccg auacaggaac gccucacugg     1320

aacaauaaag aggcccuggu ugaauuuaag gacgcucaug ccaagagaca gaccgugguc     1380

gugcucggca gccaggaggg ggcgguucac acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggccg ccuguucucc gggcaucuca agugccggcu gaaaauggac     1500

aaacuuagac ugaagggagu gagcuacucu cucuguaccg cugccuuuac auucaccaag     1560

guccccgcgg agacgcugca cggcaccgug acaguggaag uucaguaugc cgggaccgac     1620

ggaccgugca aggucccugc ucaaauggcc guggauaugc agacacugac ccccgugggc     1680

cgccucauua cggcuaaccc ugucaucaca gaguccaccg aaaacagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac aucgugauug gcguuggaga caagaaaauc     1800

acacaccauu ggcaccgguc cggcagcacc auugggaagg ccuucgaggc gaccgugaga     1860

ggagccaaac gcauggcugu ccugggcgau acagccuggg acuucggguc cgugggaggc     1920

guuuuuaauu cucucgggaa gggcauccau cagauuuucg gagcugccuu uaagagccug     1980

uucggcggga uguccugguu uucucaaauc cugaucggaa cgcuucucgu guggcugggc     2040

cugaacacca aaaaugggag cauuucccuc acaugucugg cccuuggagg cgucaugauc     2100

uuccugagca ccgcuguguc ugcggauguu gggugcuccg uggacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8977
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 3 opt

<400>  8977
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaacacaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccauuc uacaagaaag      420

cugcagaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ccugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucgcccugg ucgcuguggc caucgcuugg      540

cuccugggga gcucuacguc ccagaagguu auuuaccuug ugaugauccu gcucauugcc      600

ccugcguaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaacau ggguggacgu cguucuggaa cauggcgggu gugugaccgu gauggcccaa      720

gauaaaccca cagucgacau cgagcuugug accaccacag uuagcaacau ggcugaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacg      840

cagggagagg ccuaucugga caagcagagc gauacccagu acgucugcaa aagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uucaacccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc cagcacucug ggaugauugu caaugauauc     1080

ggacaugaaa cggacgagaa cagagcuaag guggagguca caccuaauag cccgcgcgcg     1140

gaagccaccc uuggcggcuu cgggucccug ggacucgauu gugagccccg gaccggccug     1200

gacuuuucug aucuuuacua ucugacaaug aacaauaaac acuggcucgu gcauaaggaa     1260

ugguuccacg acauuccucu gcccuggcac gcuggggccg auaccggaac accucauugg     1320

aacaauaaag aggcccuggu ugaauuuaag gacgcucacg ccaagagaca gacggugguc     1380

gugcucggca gccaggaggg ggcgguucau accgcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaaaggccg ccuguucucc gggcaccuca agugccggcu gaaaauggac     1500

aaacuuagac ugaaaggagu gagcuacucu cucuguacag cugucugcac cgccgcgaag     1560

gugcccgccg agacacugca uggcaccgug acgguugaag uccaauaugc ugggaccgac     1620

ggaccgugua aggugccugc ccagauggcu guggauaugc agacacugac ccccgucggc     1680

cgccucauca cagccaaccc ugugaucacc gaguccacgg aaaacagcaa aaugaugcug     1740

gagcuugacc cucccuuugg ggauucuuac auuguuaucg gcgugggaga caagaaaauu     1800

acacaccacu ggcaucgguc cggcagcacc aucgggaagg cguucgaggc cacagucaga     1860

ggagcuaaac gcauggccgu gcugggcgau accgcuuggg acuuuggguc cguuggaggc     1920

guguucaauu cucucgggaa gggcauucac caaaucuucg gagccgccuu uaagagccug     1980

uucggcggga uguccugguu uucucagauc cugauuggaa cccuucucgu cuggcugggc     2040

cugaacacaa agaaugggag caucucccuc acgugccugg cucuuggagg cgugaugauu     2100

uuccugagca ccgcgguuuc ugccgaugug ggguguuccg ucgacuuuag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8978
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 3 opt

<400>  8978
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacgccagca ucgggauugu gucccugcuc cugaccacag ucauggcggc ugaaaucacg      120

cgcagaggau cugccuacua uauguaccuu gaucggagcg acgccggcaa ggcuauuucc      180

uucguuacca cacugggggu gaauaaaugc cacgugcaga ucauggaucu cggacauaug      240

ugugacgcca ccaugucuua ugagugcccc augcuggaug aaggcgucga gcccgacgau      300

guggacuguu ggugcaacac aaccagcaca uggguugugu acgggaccug ucaccauaag      360

aaaggagaag cucgcagauc ccggcgcgcc gucacgcuuc cgucucacag caccagaaag      420

cugcaaacac ggucccagac cuggcucgag agccgcgagu auacaaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucgcgcugg uugccguggc uaucgccugg      540

cuccugggau cuuccaccag ccagaagguc auuuaccuug ugaugauccu gcucauugcu      600

ccugccuauu cuauccggug cauuggggug uccaaucgcg auuuugucga gggaaugagc      660

ggcgggacgu ggguugacgu ggugcuggaa cacggaggcu gugucacagu gauggcgcag      720

gauaaaccca ccguugacau cgagcuugug acaaccaccg ucucuaacau ggccgaggug      780

agauccuacu gcuacgaagc uagcaucucc gauauggccu cugacagccg guguccuaca      840

caaggggagg cuuaucugga caagcagucc gauacgcagu acguuugcaa aagaacccuc      900

guggaccgcg gauggggcaa uggcuguggg cuguucggaa agggcagccu ggucacaugc      960

gccaaguuua ccugcucuaa aaagaugaca gggaaaucca uucagcccga aaaccucgag     1020

uaucggauca ugcugagcgu gcacggaucu caacauagcg gcaugauugu caaugaugaa     1080

aacagagcca agguggaggu uaccccuaau uccccgcgcg cugaggcgac gcuuggggga     1140

uucggcucuc ugggccucga cugugaaccc cggacagggc uggauuuuag cgaccuuuac     1200

uaucugacca ugaacaauaa acacuggcuc gugcauaagg agugguucca cgauaucccu     1260

cugcccuggc acuccggagc cgacaccgaa acaccucauu ggaacaauaa agaggcucug     1320

gucgaauuua aggaugccca cgccaagaga cagaccgugg uugugcucgg cagccaggag     1380

ggggcugucc auacagcccu ggcgggagcu cuugaggccg aaauggacgg cgccaaaggg     1440

cgccugucuu ccggccaccu caagugccgg cugaaaaugg auaaacuuag acugaaagga     1500

gugagcuacu cucucuguac ggcugccuuc accuuuacaa aggugcccgc ugagacccug     1560

cauggcacag uuaccgucga agugcaguau gccgggcgcg acggaccgug caaggugccu     1620

gcgcaaaugg ccgucgacau gcagacgcug acccccgugg gccggcucau uacagcuaac     1680

ccuguuauca ccgaguccac agaaaacagc aaaaugaugc uggagcuuga uccucccuuc     1740

ggggacuccu acaucgugau uggagucggc gauaagaaaa ucacccacca cuggcauaga     1800

ucugggagca uuaucggcaa ggccuuugag gcuacggugc gcggagccaa acggauggcg     1860

guucugggcg acacagccug ggauuucggg uccgugggag gcgucuucaa uucucucggg     1920

aagggcauuc accagaucuu uggcgcugcc uucaagagcc uguuuggggg cauguccugg     1980

uucagccaaa uccugauugg aacccuucuc guguggcugg gccugaacac aaagaauggg     2040

ucuaucuccc ucaccugucu ggcucuugga ggcguuauga uuuuucugag caccgccgug     2100

ucugccgacg ucgggugcuc cguggauuuc agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8979
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 3 opt

<400>  8979
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauugu gggacugcuc cugacaacgg ccauggcggc ugaaaucacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuauuagc      180

uucgccacaa cccugggagu caauaaaugc cacguucaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aaggggugga gcccgacgau      300

guggacuguu ggugcaacac cacaucuacc ugggucgugu acggaacgug ucaccauaag      360

aaaggcgaag cccgcagaag ccggcgcgcg guuacccuuc cgucccacuc uacaagaaag      420

cugcaaaccc ggagccagac auggcucgag ucccgcgagu auaccaagca ucugaucaaa      480

guggaaaauu ggauuuuuag aaaccccggc uucgcccugg ucgcuguggc caucgcuugg      540

cuccugggga gcucuacguc ccagaagguu auuuaccuug ugaugauccu gcucauugcc      600

ccugcguaua gcauccggug cauuggaguc ucuaaucgcg auuuugugga gggcaugucc      660

gggggaacau ggguggacgu cguucuggaa cacggcgggu gugugaccgu gauggcccag      720

gauaaaccca cagucgacau cgagcuugug accaccacag uuagcaacau ggcugaggug      780

agaucuuacu gcuacgaagc cuccaucagc gauauggcuu ccgacucucg guguccuacg      840

caaggagagg ccuaucugga caagcagagc gauacccagu acgucugcaa aagaacacuc      900

guggaccgcg gcuggggcaa ugggugugga cuguucggca aggggucccu gguuaccugc      960

gccaaguuua caugcagcaa aaagaugacc ggaaaaucua uucagcccga aaaccucgag     1020

uaucggauca ugcuguccgu gcacggcagc caacauucug ggaugauugu caaugaugaa     1080

aacagagcua agguggaggu cacgccuaau agcccgcgcg cggaggccac acuuggaggc     1140

uucggcuccc uggggcucga cugugaaccc cggaccggac uggauuuuuc ugaccuuuac     1200

uaucugacca ugaacaauaa acacuggcuc gugcauaagg agugguucca cgauaucccu     1260

cugcccuggc acgcuggcgc cgacacaggg accccucauu ggaacaauaa agaagcccug     1320

guugaguuua aggaugcuca cgccaagaga cagacagugg ucgugcucgg aagccaggaa     1380

ggcgcgguuc auacggcucu ggccggggcc cuugaggcug agauggacgg cgccaaagga     1440

cgccuguucu ccggccaccu caagugccgg cugaaaaugg auaaacuuag acugaaaggg     1500

gugagcuacu cucucuguac cgcugccuuu acauucacca agguccccgc ggaaacacug     1560

cauggaaccg ugacggugga gguucaguau gccggcaccg acgggccgug caaggucccu     1620

gcucaaaugg ccguggacau gcagacacug acccccgugg gacgccucau uacagcuaac     1680

ccugucauca ccgaauccac ggagaacagc aaaaugaugc uggaacuuga uccucccuuu     1740

ggcgacucuu acaucgugau ugggguuggc gauaagaaaa ucacacacca cuggcaucgg     1800

uccggaagca ccauuggcaa ggccuucgag gcgacaguga gaggggccaa acgcauggcu     1860

guccugggag acaccgccug ggauuucggc uccguugggg gaguguuuaa uucucucggc     1920

aaggggaucc accagauuuu cggcgcugcc uuuaagagcc uguucggagg cauguccugg     1980

uuuucucaaa uccugaucgg gacccuucuc guguggcugg gacugaacac aaagaauggc     2040

agcauuuccc ucacgugucu ggcccuuggg ggagucauga ucuuccugag caccgcugug     2100

ucugcggacg uuggcugcuc cguggauuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  8980
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 3 opt

<400>  8980
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa cccugggagu gaauaaaugc cacguccaga ucauggaucu cggccauaug      240

ugugacgcua caauguccua ugagugcccc augcuggaug aagggguuga gccugacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaaa cccgcagaag ccggcgcucc gugucucuua gauaucacua cacacggaag      420

cugcaaaccc gcagccagac auggcucgag uccagagagu auaagaaaca ucugauuaug      480

guugaaaauu ggaucuuucg gaacccgggc uucgccauug ugagcgucgc gaucaccugg      540

cugauggggu cucucacguc ccagaaggug auuuaccugg uuaugaucgu gcuuauuguc      600

cccgccuaca gcaucucuug caucggagug uccaaucgcg aucuggugga gggcaugagc      660

gggggaacau gggucgacgu ugugcucgaa cacggcgggu gugugaccga gauggcucag      720

gauaaaccua cagucgacau ugagcuggug accaugaccg uuucuaacau ggccgaagug      780

agauccuauu gcuacgaggc uagccuuucc gauauggccu cugcgagccg gugucccaca      840

caaggagaac cuucccugga caagcagagc gacacgcagu cugucugcaa aagaacccuc      900

ggcgaucgcg gcugggggaa uggauguggc aucuuuggga agggaucccu ggugacaugc      960

agcaaguuua ccugcuguaa aaagaugccc ggcaaaucua uucagccuga gaaccuggaa     1020

uaucggauca ugcucccggu ucacgggagc caacauuccg gaaugauugu gaaugacauc     1080

ggccacgaga cagaugagaa cagagccaag gucgaaguga cccccaauuc uccucgcgcu     1140

gaggccacgc ugggcggguu uggaagccuu ggccuggacu gcgaaccccg gacagggcuc     1200

gauuucuccg accuguacua ucuuaccaug aacaauaaac auuggcuggu ccacaaggag     1260

ugguuucacg auaucccucu ccccuggcau gcuggagccg acaccggcac accgcacugg     1320

aacaauaaag aagcccuggu ggaguucaag gaugcucaug cgaagagaca gaccguugug     1380

guccugggga gccaggaggg cgccgugcac acagcucucg ccggagcccu ggaagcugag     1440

auggacggcg ccaaagggcg ccuuuucucu ggacaucuga agugucggcu caaaauggau     1500

aagcugagac uuaaaggcgu uuccuacagc cugugcacgg cggcuuucac cuuuacaaag     1560

gugccugccg aaacccucca cgggacaguc accguggagg ugcagucugc cggaacggac     1620

ggccccugua agguuccugc ucaaauggcc gucgacaugc agacccugac acccgugggg     1680

cgccugauua ccgcuaaccc cgugaucaca gaauccaccg agaacagcaa aaugaugcuc     1740

gagcuggauc cuccguucgg cgacuccuau auugucaucg gagugggcga uaagaaaauu     1800

acgcaccauu ggcaccgguc ugggagcaca aucggaaagg ccuuugaagc gaccguuaga     1860

ggcgccaaac gcauggcugu gcuuggggac acagccuggg auuucggauc cgucggcggg     1920

guguucaauu cucugggcaa gggaauccau cagauuuuug gcgcugccuu caagagccuc     1980

uuugggggaa uguccugguu cagccaaauc cugauuggca cccugcuugu uuggcucggg     2040

cugaacacca aaaauggauc uaucucccug acaugccucg cgcugggcgg ggugaugauu     2100

uuucuuagca cggccgucuc ugcugacgug ggcuguuccg uugauuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  8981
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 3 opt

<400>  8981
gggagaaagc uuaccaugag aaucauuaac gcccggaagg agcgcaaaag acggggcgcu       60

gacaccagca ucgggauuau cggacugcuc cugacaacgg ccauggcggc ugaaauuacc      120

cgcagaggcu ccgccuacua uauguaccuu gaucggucug acgccgggaa ggcuaucagc      180

uucgccacaa ccaugggagu gaauaaaugc cacguccaga ucauggaucu gggccauaug      240

ugugacgcua caauguccua ugagugcccc augcucgaug aagggguuga gccugacgau      300

guggacuguu ggugcaacac cacaucuacc uggguggucu acggaacgug ucaccauaag      360

aaaggcgaag cccgcccgag cagacgggcg gugacccugc ccucccacuc uacacgcaaa      420

cuucaaacca gaagccagac auggcuggag ucccgggagu auaccaagca ucucauuaag      480

guugaaaauu ggauccugcg caacccuggc uuuacggugg ccgcucuggc cauggcuugg      540

cuccugggga auagcacauc ucagagaguc auuuaccuug ugaugauccu gcucauugcc      600

cccgcguauu ccauccggug cauuggaguu agcaaccgcg auuucgugga gggcaugucu      660

gggggaaccu gggucgacgu ggugcuggaa cacggcgggu gugucacagu uauggcccag      720

gauaagccua ccguggacau cgagcuugug accacaacgg ucuccaauau ggcugaggug      780

agaagcuacu gcuacgaagc cucuaucucc gauauggcua gcgacucccg gugucccacc      840

caaggagagg ccuaucugga caaacagucu gauacacagu acguuugcaa gagaacccuc      900

guggaccgcg gcuggggcaa cgggugugga cuguuuggca aagggagccu ggucacaugc      960

gccaaguuua ccugcuccaa gaaaaugacg ggaaagagca uucagccuga aaaucucgag     1020

uaucggauca ugcugucugu gcacggcucc caacauagcg ggaugauugu uaacgauauc     1080

ggacacgaaa cagacgagaa uagagcuaaa guggagguca ccccgaacuc uccccgcgcg     1140

gaagccaccc uuggcggcuu cgggagccug ggacucgauu gugagccucg gacaggccug     1200

gacuucuccg aucuuuacua ucugaccaug aauaacaagc auuggcucgu gcacaaagaa     1260

ugguuucacg acauuccccu gccuuggcau gcuggggccg auacaggaac gccccacugg     1320

aauaacaagg aggcccuggu cgaguucaaa gacgcucaug ccaagagaca gaccgugguu     1380

gugcucggcu cucaggaagg ggcgguccac acagcucugg ccggcgcccu ugaggcugaa     1440

auggauggag ccaagggccg ccuguuuagc gggcaucuca aaugccggau caagauggac     1500

aaacugagac uuaagggagu guccuacagc cuguguaccg cugccuucac auuuucuaaa     1560

guuccggcgg agacccucca cggcacggug accgucgaag ugcaguaugc cgggcgcgac     1620

ggaccuugca aggugcccgc ucaaauggcc guugauaugc agacacugac cccugucggc     1680

cggcugauca cagcuaaccc cauuaucacc gaguccacgg aaaauagcag aaugaugcuc     1740

gagcuggauc ccccuuucgg ggacucuuac auugugaucg gcgugggaga caagaaaauu     1800

acacaccauu ggcaccgcuc cggcagcacc aucgggaagg ccuuugaggc gacaguccgg     1860

ggagccaaaa gaauggcugu gcuuggcgau accgccuggg acuucggguc cguuggaggc     1920

guguucaacu cucuggggaa gggcguccau cagguguuug gagcugccuu caaaagccuc     1980

uuuggcggga uguccugguu cucucaaauc cugauuggaa cccugcuugu uuggcucggc     2040

cugaauacaa agaacgggag caucucccug gccugucucg cucugggagg cgcgaugauu     2100

uuucuuagca cggccguguc ugcugauguc gggugcuccg uggacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  8982
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 3 opt

<400>  8982
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauauugugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8983
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 3 opt

<400>  8983
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccguug ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa cgcuaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu gagcaccgcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8984
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 3 opt

<400>  8984
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucggguacg agacggacga agauagagcc aagguggagg ucacaccuaa cagcccgcgg     1080

gccgaggcua cccuuggagg cuucggcucc cuggggcucg acugugaacc cagaaccgga     1140

cuggauuuuu cugaccuuua uuaccugaca augaauaaca aacacuggcu cgugcauaag     1200

gagugguucc acgauauucc ucugcccugg cacgcgggcg ccgacaccgg gacaccucau     1260

uggaauaaca aagaagcucu ggucgaguuu aaggaugccc acgccaaacg ccagacggug     1320

guugugcucg gaagccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggacg gcgccaaggg acggcuguuc uccggccacc ucaagugcag acugaaaaug     1440

gauaaacuuc gccugaaggg ggugagcuau ucucucugua cagcugccuu uaccuucaca     1500

aagguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gacgggccgu gcaaaauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacggcuca uuaccgccaa uccugugauc acggaaucca cagagaacag caagaugaug     1680

cuggaacuug auccucccuu uggcgacucu uacaucguga uuggggucgg cgauaagaaa     1740

aucacccacc acuggcauag auccggaagc acaauuggca aggcuuucga ggccaccgug     1800

cgcggggcua aacggauggc cguucuggga gacaccgcgu gggauuucgg cuccgucggg     1860

ggaguguuua auucucucgg caaggggauc caccagauuu ucggcgccgc uuuuaaaagc     1920

cuguucggag gcauguccug guuuucucaa auccugaucg ggacacuucu cguguggcug     1980

ggacugaaca cgaagaacgg cagcauuucc cucaccuguc uggcccuugg gggaguuaug     2040

aucuuccuga gcacagcugu gucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8985
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 3 opt

<400>  8985
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8986
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 3 opt

<400>  8986
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucgugaacg acacggggua cgagacagau gaaaauagag ccaaggucga ggugaccccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaacag gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caaacacugg     1200

cucguccaua aggagugguu ccacgauauu ccucugcccu ggcacgcggg cgccgacaca     1260

gggacgccuc auuggaauaa caaagaagcu cugguggagu uuaaggaugc ccacgccaaa     1320

cgccagaccg uugugguccu cggaagccag gaaggcgcug ugcauacagc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggaaagcugu ucuccggcca ccucaaaugc     1440

cggcugaaga uggauaaacu uagacugaag gggguuagcu auucucucug uaccgcugcc     1500

uuuacauuca ccaaagugcc cgcugaaacg cugcauggaa ccgucacagu ggaggugcag     1560

uacgccggca ccgacgggcc gugcaagauc ccuguucaaa uggcggucga caugcagaca     1620

cugacccccg ugggacgccu cauuacggcc aauccuguga ucacagaauc caccgagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu cauuggggug     1740

ggcgauaaaa agaucacaca ccacuggcau cgguccggaa gcaccauugg caaggcuuuc     1800

gaggccaccg uuagaggggc uaaacgcaug gccgugcugg gagacacagc gugggauuuc     1860

ggcuccgucg ggggaguguu uaacucucuc ggcaaaggga uccaccagau uuucggcgcc     1920

gcuuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauccugau cgggacgcuu     1980

cucguuuggc ugggacugaa uaccaagaac ggcagcauuu cccucacaug ucuggcccuu     2040

gggggaguga ugaucuuccu gagcaccgcu gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8987
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 3 opt

<400>  8987
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucaca gaaagaaagg agaggcccgg cgcagcagac gggcugugac ccuuccgucc      360

cauucuacac gcaagcugca aaccagaagc cagacauggc ucgaaucccg ggaguauacc      420

aaacaccuga ucaagguuga gaacuggauc uuucgcaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu agaugcaucg gggugucuaa ccgggacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcaugg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgcuc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucuagauguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaacgga cacucgucga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauauag aauuaugcug uccguucacg gaagccaaca cucuggcaug     1020

aucgugaacg acacggggua cgagacagau gaaaaucggg ccaaggucga ggugaccccu     1080

aacagcccga gagccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

ccccgcacag gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caaacauugg     1200

cucguccaca aggagugguu ccaugauauu ccucugcccu ggcacgcggg cgccgacaca     1260

gggacgccuc acuggaauaa caaagaagcu cugguggagu uuaaggaugc ccaugccaaa     1320

cggcagaccg uugugguccu cggaagccag gaaggcgcug ugcacacagc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggaaagcugu ucuccggcca ucucaaaugc     1440

agacugaaga uggauaaacu ucgccugaag gggguuagcu auucucucug uaccgcugcc     1500

uuuacauuca ccaaagugcc cgcugaaacg cugcacggaa ccgucacagu ggaggugcag     1560

uacgccggca ccgacgggcc gugcaagauc ccuguucaaa uggcggucga caugcagaca     1620

cugacccccg ugggacggcu cauuacggcc aauccuguga ucacagaauc caccgagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu cauuggggug     1740

ggcgauaaaa agaucacaca ucacuggcac agauccggaa gcaccauugg caaggcuuuc     1800

gaggccaccg uucgcggggc uaaacggaug gccgugcugg gagacacagc gugggauuuc     1860

ggcuccgucg ggggaguguu uaacucucuc ggcaaaggga uccaucagau uuucggcgcc     1920

gcuuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauccugau cgggacgcuu     1980

cucguuuggc ugggacugaa uaccaagaac ggcagcauuu cccucacaug ucuggcccuu     2040

gggggaguga ugaucuuccu gagcaccgcu gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8988
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 3 opt

<400>  8988
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

acggugaacg acaucgggua cgagacagau gaaaauagag ccaaggucga ggugaccccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaacag gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caaacacugg     1200

cucguccaua aggagugguu ccacgauauu ccucugcccu ggcacgcggg cgccgacaca     1260

gggacgccuc auuggaauaa caaagaagcu cugguggagu uuaaggaugc ccacgccaaa     1320

cgccagaccg uugugguccu cggaagccag gaaggcgcug ugcauacagc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggaaagcugu ucuccggcca ccucaaaugc     1440

cggcugaaga uggauaaacu uagacugaag gggguuagcu auucucucug uaccgcugcc     1500

uuuacauuca ccaaagugcc cgcugaaacg cugcauggaa ccgucacagu ggaggugcag     1560

uacgccggca ccgacgggcc gugcaagauc ccuguucaaa uggcggucga caugcagaca     1620

cugacccccg ugggacgccu cauuacggcc aauccuguga ucacagaauc caccgagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu cauuggggug     1740

ggcgauaaaa agaucacaca ccacuggcau cgguccggaa gcaccauugg caaggcuuuc     1800

gaggccaccg uuagaggggc uaaacgcaug gccgugcugg gagacacagc gugggauuuc     1860

ggcuccgucg ggggaguguu uaacucucuc ggcaaaggga uccaccagau uuucggcgcc     1920

gcuuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauccugau cgggacgcuu     1980

cucguuuggc ugggacugaa uaccaagaac ggcagcauuu cccucacaug ucuggcccuu     2040

gggggaguga ugaucuuccu gagcaccgcu gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8989
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 3 opt

<400>  8989
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga uuaaggugga gaacuggauc uuucggaauc ccggcuucac gcuggucgcc      480

guggcgaucg ccuggcuccu gggcagcucu acaucccaga agguuauuua ccuugugaug      540

auccugcuca uugcuccugc cuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gaccugggug gaugucguuc uggagcacgg aggcugugug      660

acagugaugg cucaggacaa acccaccguc gauaucgaac uugugaccac aacgguuagc      720

aauauggccg aggugcgguc uuacugcuac gaggcgucca uuagcgacau ggccuccgau      780

ucucgcuguc cuacccaagg ggaggcuuau cuggacaagc agagcgacac acaguacguc      840

ugcaaaagaa cccucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua caugcgccaa auuuaccugc agcaagaaga ugacggggaa aucuauccag      960

cccgaaaauc ucgaauaucg caucaugcug uccgugcacg gaagccaaca uucuggcaug     1020

auugucaacg acgagaauag agcuaaggug gaagucacac cuaacagccc gcgggccgag     1080

gccacccuug ggggauucgg cucccugggc cucgauugug agcccagaac cgggcuggac     1140

uuuucugauc uuuacuaucu gacaaugaau aacaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaca ucccucugcc cuggcacgcu ggagcggaua ccggcacacc ucauuggaau     1260

aacaaagagg cccugguuga auuuaaggac gcucacgcca aacgccagac gguggucgug     1320

cucgggagcc aggagggagc cguucauacc gcucuggccg gcgcgcuuga agcugagaug     1380

gauggggcca agggccggcu guucuccgga caccucaagu gcagacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucucuc uguacagccg cuuuuaccuu cacaaagguc     1500

cccgccgaga cccugcaugg gacggugacc guggaaguuc aguaugcugg aacagauggc     1560

ccgugcaaag ucccugccca aauggcggug gacaugcaga cccugacacc cguggggcgg     1620

cucauuaccg ccaauccugu caucacggag uccacagaaa acagcaagau gaugcuggag     1680

cuugacccuc ccuuuggaga uucuuacauu gugaucggcg uuggggacaa gaaaaucacc     1740

caccacuggc auagauccgg cagcacaauu ggaaaggcuu ucgaagccac cgugcgcggc     1800

gcuaaacgga uggccguccu gggggauacc gcgugggacu ucggauccgu gggcgggguu     1860

uuuaacucuc ucggaaaggg cauccaccag auuuucgggg ccgcuuuuaa aagccuguuc     1920

ggcggaaugu ccugguuuuc ucaaauccug auuggcacac uucucgugug gcuggggcug     1980

aauacgaaga acggaagcau cucccucacc ugucuggccc uuggcggggu caugaucuuc     2040

cugagcacag cugugucugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  8990
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 3 opt

<400>  8990
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucggguacg agacggacga aaacagagcc aagguggagg ucacaccuaa uagcccgcgg     1080

gccgaggcua cccuuggagg cuucggcucc cuggggcucg auugugaacc cagaaccgga     1140

cuggacuuuu cugaucuuua uuaccugaca augaacaaua aacacuggcu cgugcauaag     1200

gagugguucc acgacauucc ucugcccugg cacgcgggcg ccgauaccgg gacaccucau     1260

uggaacaaua aagaagcucu ggucgaguuu aaggacgccc acgccaaacg ccagacggug     1320

guugugcucg gaagccagga aggcgcuguc cauaccgccc uggcgggggc ucuugaggcc     1380

gagauggaug gcgccaaggg aaagcuguuc uccggccacc ucaaaugccg gcugaagaug     1440

gacaaacuua gacugaaggg ggugagcuau ucucucugua cagcugccuu uaccuucaca     1500

aaaguucccg cugaaacccu gcauggaacg gugaccgucg aggugcagua cgccggcaca     1560

gaugggccgu gcaagauccc ugugcaaaug gcgguugaca ugcagacccu gacacccguc     1620

ggacgccuca uuaccgccaa cccugugauc acggaaucca cagagaauag caagaugaug     1680

cuggaacuug acccucccuu uggcgauucu uacaucguga uuggggucgg cgacaaaaag     1740

aucacccacc acuggcaucg guccggaagc acaauuggca aggcuuucga ggccaccgug     1800

agaggggcua aacgcauggc cguucuggga gauaccgcgu gggacuucgg cuccgucggg     1860

ggaguguuua acucucucgg caaagggauc caccagauuu ucggcgccgc uuuuaagagc     1920

cuguucggag gcauguccug guuuucucaa auccugaucg ggacacuucu cguguggcug     1980

ggacugaaca cgaagaaugg cagcauuucc cucaccuguc uggcccuugg gggaguuaug     2040

aucuuccuga gcacagcugu gucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  8991
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 3 opt

<400>  8991
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggccgcggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaaaaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8992
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 3 opt

<400>  8992
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggccuggc ucucgccgcg      480

gccgcuaucg ccuggcugcu uggcuccagc acgucucaga aagucaucua ucuggugaug      540

auucuccuga ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuc      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uugaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uggugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cucgacaagc agucugacac ccaguauguu      840

ugcaaacgga cacuggugga ucgcggaugg ggcaauggcu gugggcuguu uggaaagggc      900

agccucguga ccugcgccaa auucgccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc uggaguacag aaucaugcuu ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuggga ggcuuuggca gccucgggcu ggacugugag     1140

ccucgcaccg gacuugauuu cuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ucacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cucguggagu ucaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu gggaucucag gaaggcgcgg ugcauaccgc ucuugccggg     1380

gcccuggagg cugaaaugga cggcgccaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gcggcucaaa ggggucagcu auucucugug uacagcugcc     1500

uuuaccuuca caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cucacaccug ugggaagacu gaucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggaucccccu uuuggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccucg gagauaccgc cugggacuuu     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuucaaaa gccuguuugg aggcaugucc ugguucucuc aaauucucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggaguuc ugauuuuucu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8993
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 3 opt

<400>  8993
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacugguau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaucagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8994
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 3 opt

<400>  8994
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga cacccagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa caccaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcacagcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8995
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 3 opt

<400>  8995
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg agauccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8996
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 3 opt

<400>  8996
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacaggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cuggcucaaa uggccgugga caugcagacc     1620

cucacacccg ugggaagacu gaucaccgcu aacccuguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggauccgccc uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccucg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuuuucgg aggcaugucc ugguuuucuc aaauucucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8997
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 3 opt

<400>  8997
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuacccu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacacg      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acaucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gaccuggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugaccac aacggucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacccaagg ggaggcuuac cuggacaagc agucugacac acaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccgggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acacggggca ugaaacagau gagaaccggg cuaaggugga gauuaccccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcacag gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacgcccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ucgugguucu cggaucucag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcuccguug ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa caccaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggagugc ucauuuuccu gagcacagcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8998
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 3 opt

<400>  8998
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgug gucucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  8999
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 3 opt

<400>  8999
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccgcuugggu cguguauggg      300

accugucauc acaaaaaggg agaagccaga cggucucgca gagcuguuac gcuucccagc      360

cauuccaccc ggaaacugca aacacgcccu cagaccuggc ucgagucuag agaguacaca      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc ccggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcagcucc accagccaga aagucaucua ucuugugaug      540

auucugcuca ucgccccugc guacucuauu agaugcaucg ggguuuccaa ucgggacuuu      600

guggagggaa ugagcggcgg gacguggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cccaggacaa gcccaccgug gauauugagc uugugacaac caccgucucu      720

aacauggcug aggugcgcuc cuacugcuau gaagccagca ucucugacau ggcuuccgau      780

agcagauguc cuacacaagg ggaggccuac cuggacaagc aguccgacac gcaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

ucucugguca caugcgccaa auuugcuugc agcaagaaaa ugaccgggaa guccauucag      960

ccggaaaacc ucgaguacag aaucaugcug agcgugcacg gaucucaaca cuccggcaug     1020

aucguuaaug acacagggca ugaaaccgau gagaaccggg cgaaggugga gauuacgccc     1080

aauagcccua gagccgaagc uacacuugga ggcuucggcu cucuggggcu cgacugugag     1140

ccccgcaccg gacuggauuu uagcgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccucugcccu ggcacgccgg cgccgacaca     1260

gggaccccgc auuggaacaa uaaagaggcu cugguggaau uuaaggaugc ccacgcgaaa     1320

cggcagacag ucgugguucu cggaucccag gagggcgcug ugcauacggc ccuggccggg     1380

gcucuugagg ccgaaaugga cggcgcuaag ggaagacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucuccu auagccucug uaccgccgcg     1500

uucacauuua ccaagauucc ugccgagaca cugcauggaa ccgugacggu ugaagugcag     1560

uacgcuggca ccgacgggcc cugcaaaguc ccugcccaaa uggcugugga caugcagaca     1620

cugacccccg ugggaagacu caucacagcc aaccccguua uuaccgaguc uacggaaaac     1680

uccaagauga ugcuggagcu ugauccuccg uucggcgaca gcuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcucuggau ccaccaucgg caaggcguuu     1800

gaagccacag ugagaggggc uaaacggaug gccguccugg gagauaccgc uugggacuuc     1860

ggcagcguug ggggagcccu caauucccug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcucuauca gccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu guccacggcc gugagcgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9000
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 3 opt

<400>  9000
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucuaaaa aggucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaggg ggaggcuuac cuggacaaac aaucugacac ccaguauguu      840

ugcaagcgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaaaggc      900

agccugguga ccugcgccaa guuugccugc uccaaaaaga ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccagca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaagugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaagcacugg     1200

cucguccaua aagaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaggaggcc cugguggaau uuaaagaugc ucacgccaag     1320

cggcaaacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaaa ggaagacuga gcuccggcca ccucaagugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaaaauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaagguc cccgcucaga uggccgugga caugcaaacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaga agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaagccuuu     1800

gaagcgaccg ugagaggggc caagcggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaaaggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc agauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaaaaau ggcagcaucu cccugaugug ucuugcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9001
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 3 opt

<400>  9001
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgagacaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacucuauu cggugcaucg ggguuuccaa ucgcgacuuu      600

guggagggaa ugagcggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucu      720

aacauggccg aggugagauc cuacugcuau gaagcuagca ucucugacau ggccuccgau      780

agccgguguc ccacgcaagg ggaggcuuac cuggacaagc aguccgacac ccaguauguu      840

ugcaaacgca cacucgugga uagaggaugg ggcaauggcu gugggcuguu cggaaagggc      900

ucucugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa guccauucag      960

ccugaaaacc ucgaguaccg gaucaugcug agcgugcacg gaucucaaca cuccggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaacagag cuaaggugga gauuacaccg     1080

aauagccccc gcgcggaagc cacccuugga ggcuucggcu cucuggggcu cgacugugag     1140

ccucggaccg gacuggauuu uagcgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

agacagacgg ucgugguucu cggaucccag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggacgccugu cuagcggcca ccucaaaugc     1440

cggcugaaga uggauaagcu gagacugaaa ggggucuccu auagccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggacgccu caucaccgcu aaccccguua uuacggaguc uacagaaaac     1680

uccaagauga ugcuggagcu ugaucccccu uucggcgaca gcuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cggucuggau ccacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacgcaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcagcgucg ggggagcucu caauucccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcucuauca gccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu guccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9002
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 3 opt

<400>  9002
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaacccag gagggcgcgg ugcauacagc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucuccu auagccucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcu aaccccguua uuacagaguc uaccgaaaac     1680

uccaagauga ugcuggagcu ugaucccccu uucggcgaca gcuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcucuggau ccaccaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauacagc cugggacuuc     1860

ggcagcgucg ggggagcucu caauucccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauucuuau cgggacgcuc     1980

cugauguggc ugggacucaa caccaagaau ggcucuauca gccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu guccacagcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9003
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 3 opt

<400>  9003
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacucccug agaugcauug ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uugaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauaucgagc uggugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cucgacaagc agucugacac ccaguauguu      840

ugcaaacgga cacuggugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccucguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc uggaguacag aauuaugcuu ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gaucacaccc     1080

aauucuccga gagcggaagc cacccuggga ggcuucggca gccucgggcu ggacugugag     1140

ccucgcaccg gacuugauuu uuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cucguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu gggaucucag gagggcgcgg ugcauaccgc ucuugccggg     1380

gcccuggagg cugaaaugga cggcgccaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gcggcucaaa ggggucagcu auucucugug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cucacaccug ugggaagacu gauuaccgcu aaccccguua ucacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggaucccccu uucggcgacu cuuacauugu caucggggug     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccucg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccucau ugggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggaguuc ugaucuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9004
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 3 opt

<400>  9004
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccggauuga aaauuggauc uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagugauuua ucuugucaug      540

auccugcuca uugcucccgc cuacuccauc agaugcauug gggugagcaa ucgggacuuu      600

guugagggaa ugucuggcgg gacaugggug gaugucgugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucucag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggagugc ucaucuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9005
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 3 opt

<400>  9005
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu cugcgcaacc cuggcuuugc ucucgccgcg      480

gccgcuaucg ccuggcugcu uggcuccagc acgucucaga aagucaucua ucuggugaug      540

auucuccuga ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuc      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uugaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uggugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cucgacaagc agucugacac ccaguauguu      840

ugcaaacgga cacuggugga ucgcggaugg ggcaauggcu gugggcuguu uggaaagggc      900

agccucguga ccugcgccaa auucgccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc uggaguacag aaucaugcuu ucuguccacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuggga ggcuuuggca gccucgggcu ggacugugag     1140

ccucgcaccg gacuugauuu cuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ucacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cucguggagu ucaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu gggaucucag gaaggcgcgg ugcauaccgc ucuugccggg     1380

gcccuggagg cugaaaugga cggcgccaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gcggcucaaa ggggucagcu auucucugug uacagcugcc     1500

uuuaccuuca caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cucacaccug ugggaagacu gaucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggaucccccu uuuggcgacu cuuacaucgu caucgggauu     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuc     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcucg gagauaccgc cugggacuuu     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuucaaaa gccuguuugg aggcaugucc ugguucucuc aaauccucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcauuu cccuuaugug ucuggcucuc     2040

gggggagugc ugaucuuucu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9006
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 3 opt

<400>  9006
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uuguguccac caccgucagc      720

aacauggccg aggugcgcuc uuacugcuau gaagcuacaa ucuccgacau ugccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auuguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gaucacaccg     1080

aauagcccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg cggucgugcu cggaucucag gagggcgcug uucauaccgc ccuggccggg     1380

gcucuugagg ccgaaaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcg     1500

uucaccuuua caaagauccc ggccgagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgcuggca cagacgggcc uugcaaagug cccgcccaaa uggcugucga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcc aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu uauugggguc     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggcguuu     1800

gaagccaccg ugagaggggc uaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggagugc ucaucuuccu gagcaccgcc guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9007
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 3 opt

<400>  9007
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaacugcag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cucacaccug ugggaagacu gaucaccgcu aaccccguga uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggaucccccu uucggcgacu cuuacaucgu uaucgggguc     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcucg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuuuucgg aggcaugucc ugguuuucuc aaauucucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggagugc ugauuuuccu gagcaccgcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9008
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 3 opt

<400>  9008
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc caucgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu cauuggggug     1740

ggcgagaaaa agaucacaca ccacuggcau cgcuccggaa gcaccauugg caaggccuuu     1800

gaagcgacag ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcagcauuu cccugaugug ucuugcucuu     2040

gggggaguuc ucaucuuccu gagcacggcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9009
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 3 opt

<400>  9009
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgucaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggugagcu auucucucug uacagccgcu     1500

uucaccuuua caaagauucc ggccgagacc cugcauggaa cgguuaccgu ggaaguccag     1560

uacgcgggca cagacgggcc uugcaaagug cccgcccaaa uggcugugga caugcagacc     1620

cugacaccug uuggaagacu caucaccgcc aaccccguca uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu gaucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggcuuuu     1800

gaagccaccg ucagaggggc gaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccguug ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu ccuucaugug ucuggcccuu     2040

gggggagugc ugauuuuucu cagcaccgcu gucucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9010
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 3 opt

<400>  9010
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaaaacuga gcuccggcca ccucaagugc     1440

agacugaaga uggauaaacu ucgccugaag ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaaaauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaagguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggacggcu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaga agauuaccca ccacuggcau agauccggaa gcacaaucgg caaagccuuu     1800

gaagcgaccg ugcgcggggc caagcggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaaaggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaaaaau ggcagcaucu cccugaugug ucuugcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9011
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 3 opt

<400>  9011
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caaggaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaca aaugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau ccaccugggu cguguauggg      300

acgugucauc acaagaaagg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaagcugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aaacaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aggucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ccgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aauauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaaac agucugacac ccaguauguu      840

ugcaagcgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaaaggc      900

agccugguga ccugcgccaa guuugccugc uccaaaaaga ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaagugga gauuacaccg     1080

aacucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaauaa caagcacugg     1200

cucguccaua aagaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaggaggcc cugguggaau uuaaagaugc ucacgccaag     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaaa ggaagacuga gcuccggcca ccucaagugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaaaauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaagguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaucccguua uuacggaguc cacagaaaac     1680

agcaaaauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaga agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaagccuuu     1800

gaagcgaccg ugagaggggc caagcggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caacucucug ggcaaaggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaaaaac ggcagcaucu cccugaugug ucuugcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9012
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 3 opt

<400>  9012
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacaggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccca gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaggc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugaag cugagaugga uggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggacaaacu ucggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggaaacc cugcauggaa cggugaccgu ugaggugcag     1560

uacgccggca cagaugggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaauc cacagagaac     1680

agcaagauga ugcuggagcu ugacccgccu uucggcgauu cuuacaucgu caucggggug     1740

ggcgaaaaga agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaggcgaccg ugagaggggc caaacggaug gcuguccugg gagacaccgc cugggauuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9013
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 3 opt

<400>  9013
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacaggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccca gagcggaagc cgcucuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgccgg cgccgacaca     1260

gggaccccgc auuggaacaa uaaagaggcu cugguggaau uuaaggaugc ccacgcgaaa     1320

cggcagacag ucgugguucu cggaucucag gagggcgcug ugcauacggc ccuggccggg     1380

gcucuugagg ccgaaaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgccgcg     1500

uucacauuua ccaagauucc ggccgagaca cugcauggaa ccgugacggu ugaagugcag     1560

uacgcuggca ccgacgggcc uugcaaaguc cccgcccaaa uggcugugga caugcagaca     1620

cugaccccug ugggaagacu caucacagcc aaccccguua uuaccgaguc cacggaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggcguuu     1800

gaagccacag ugagaggggc uaaacggaug gccguccugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaacccuuau ugggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggaguuc ucaucuuccu gagcaccgcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9014
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 3 opt

<400>  9014
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggcg cugacgggcc uugcaaaguc cccgcccaaa uggcugugga caugcagaca     1620

cugaccccug ugggaagacu caucacagcc aaccccguua uuaccgaguc cacggaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggcguuu     1800

gaagccacag ugagaggggc uaaacggaug gccguccugg gagauaccgc uugggacuuc     1860

ggcuccgucg ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggaguuc ucauuuuccu gagcacggcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9015
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 3 opt

<400>  9015
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gccccaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucuggc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uugaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gcccacagug gauauugagc uggugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc cuacgcaagg ggaggcuuac cucgacaagc agucugacac ccaguauguu      840

ugcaaacgga cacuggugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccucguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

cccgaaaacc uggaguacag aaucaugcuu ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccu     1080

aauucuccga gagcggaagc cacccuggga ggcuucggca gccucgggcu ggacugugag     1140

ccccgcaccg gacuugauuu uuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cugguccaua aggaaugguu ccacgauauc ccucugcccu ggcacgcugg cgccgacacc     1260

gggacaccuc auuggaacaa uaaagaggcc cucguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu gggaucucag gagggcgcgg ugcauaccgc ucuugccggg     1380

gcccuggagg cugaaaugga cggcgccaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggauaagcu gcggcucaaa ggggucagcu auucucugug uacagcugcc     1500

uucaccuuua caaagauucc cgcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc gugcaaaguc ccugcucaaa uggccgugga caugcagacc     1620

cucacacccg ugggaagacu gaucaccgcu aacccuguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuugagcu ggauccuccc uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccucg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu uaauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucucau cgggacacug     1980

cugauguggc ucggacugaa cacgaagaau ggcagcaucu cccuuaugug ucuggcucuc     2040

gggggaguuc ugauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9016
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 3 opt

<400>  9016
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau gguuagcgau      780

uccagauguc ccacgcaagg ggaggccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucgucga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgcuaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguucacg gaucccaaca cagcggcaug     1020

aucgugaaug acaccgggca ugaaacggau gagaaccggg ccaaggucga gauuacaccc     1080

aauucuccga gagcugaagc gacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauc ccccugccuu ggcacgccgg cgcugacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ccacgcuaaa     1320

cggcagacgg ugguugugcu cggaucucag gagggcgccg uccauaccgc gcuggcuggg     1380

gcccuugagg ccgaaaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugagcu auucucucug uacagccgcu     1500

uucaccuuua caaagauucc ggccgagacc cugcauggaa cgguuaccgu ggaaguccag     1560

uacgcgggca cagacgggcc uugcaaagug cccgcccaaa uggcugugga caugcagacc     1620

cugacaccug uuggaagacu caucaccgcc aaccccguca uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu gaucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggcuuuu     1800

gaagccaccg ucagaggggc gaaacggaug gccgugcugg gagauaccgc uugggacuuc     1860

ggcuccguug ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcccuu     2040

gggggagugc ucauuuuccu gagcaccgcu gucucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9017
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 3 opt

<400>  9017
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggauuagcaa ucgggacuuu      600

guugagggaa ugucuggcgg gacaugggug gaugucgugc uggaacaugg aggcugugug      660

accgucaugg cgcaggacaa gccuacaguu gauaucgagc uugugaccac cacagugucc      720

aacauggccg agguccgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

auugugaaug acaccgggca ugaaacggau gagaaccggg cuaagguuga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca uucuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uagcgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucgugcaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagacgg uggucgugcu cggaucccag gagggcgcgg uucauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu cuagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa gggguguccu auagccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggucaccgu ggaaguucag     1560

uacgccggca cagacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguga ucacggaguc uacagaaaac     1680

uccaagauga ugcuggagcu ugaucccccu uucggcgaca gcuacauugu uaucgggguc     1740

ggcgagaaaa agauuaccca ccacuggcau cgcucuggau ccacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcagcgucg ggggagcucu caauucccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauccuuau ugggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcucuauca gccugaugug ucuugcucuu     2040

gggggagugc ucauuuuccu guccaccgcg guuagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9018
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 3 opt

<400>  9018
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acacauguga ugccaccaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacacaaccu ccacgugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac acuucccagc      360

cauuccaucc ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uucgggugga aaauuggauc uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucauuua ucuugugaug      540

auccugcuca uugcucccgc cuacuccauc agaugcauug ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauaucgagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

auuguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguua ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu cauuggggug     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau cgggacacuc     1980

cugauguggc ugggacucaa cgcuaagaau ggcagcauuu cccugaugug ucuugcgcuu     2040

gggggaguuc ucaucuuccu gagcacggcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9019
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 3 opt

<400>  9019
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcucaa agaucucggc gcgccguuac ccuucccagc      360

cauuccacaa gaaaacugca gacccggucu cagacauggc ucgagagccg cgaguacacc      420

aagcaccuga ucagagugga aaauuggauu uuucggaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu cgcugcaucg ggguuagcaa uagagacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaagacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcggag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

ucccgcuguc ccacgcaggg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaaagaa cacucgugga ucggggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucaa      960

ccugaaaacc ucgaguaccg caucaugcug ucugugcacg gaucccagca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaacagag cuaaggugga gauuacaccc     1080

aauucuccgc gggcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccuagaaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cgccagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggacggcuga gcuccggcca ccucaaaugc     1440

agacugaaga uggauaagcu gcgccugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcaa     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaga uggccgugga caugcagacc     1620

cugacaccug ugggacggcu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau agauccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugcgcggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccaaau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9020
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 3 opt

<400>  9020
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca agcucgcucu cagaccuggc ucgagagcag agaguacaca      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ccuggcggcc      480

gcugccaucg cuuggcuccu gggcuccagc accucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcccccgc guacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacguggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cccaggacaa gccuaccgug gauauugagc uugugacaac caccgucucc      720

aacauggcug aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacacaagg ggaggccuac cuggacaagc agucugacac gcaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugcuugc uccaagaaaa ugaccggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acacagggca ugaaaccgau gagaaccggg cgaaggugga gauuacgccc     1080

aauucuccca gagccgaagc uacacuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgccgg cgccgacaca     1260

gggaccccgc auuggaacaa uaaagaggcu cugguggaau uuaaggaugc ccacgcgaaa     1320

cggcagacag ucgugguucu cggaucucag gagggcgcug ugcauacggc ccuggccggg     1380

gcucuugagg ccgaaaugga cggcgcuaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgccgcg     1500

uucacauuua ccaagauucc ggccgagaca cugcauggaa ccgugacggu ugaagugcag     1560

uacgcuggca ccgacgggcc uugcaaaguc cccgcccaaa uggcugugga caugcagaca     1620

cugaccccug ugggaagacu caucacagcc aaccccguua uuaccgaguc cacggaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggcguuu     1800

gaagccacag ugagaggggc uaaacggaug gccguccugg gagauaccgc uugggacuuc     1860

ggcuccguug ggggagcccu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcagcaucu cccugaugug ucuugcgcuu     2040

gggggagugc ucauuuuccu gagcacggcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9021
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 3 opt

<400>  9021
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucagc      720

aauauggccg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccguccacg gaagccaaca cucuggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacagcccca gagcggaagc cacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaagccag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaaa ggggucucuu auucccucug uacagcugcc     1500

uucaccuuua gcaagauucc ggcggagaca cugcauggaa ccgugacgga agagguucag     1560

uacgccggca ccgacgggcc uugcaaagug cccgcucaaa uggccgucga caugcagaca     1620

cugaccccug ugggaagacu caucacagcu aaucccguga uuaccgaauc uacggagaac     1680

uccaagauga ugcuggagcu ugaucccccu uucggcgaca gcuacaucgu uaucgggguc     1740

ggcgaaaaga agauuacaca ccacuggcau cgcuccggau cuaccaucgg caaggccuuu     1800

gaggcgacag ugagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcagcgucg ggggagcucu caacucccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cucuguucgg aggcaugagc ugguuuuccc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa uacaaagaac ggcagcaucu cucugaugug ucuugcucuc     2040

gggggagugc ugauuuuccu guccacggcg guuagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9022
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 3 opt

<400>  9022
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucagc      720

aauauggccg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccguccacg gaagccaaca cucuggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacagcccca gagcggaagc cacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacaccgg cgcugacaca     1260

gggacgcccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ccacgcuaaa     1320

cggcagaccg ucgugguucu cggaagccag gagggcgccg ugcauacagc gcuggcuggg     1380

gcccuugagg ccgaaaugga cggcgcuaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaaacu ucggcugaag ggggucucuu auucccucug uaccgccgcu     1500

uucacauuua ccaagauucc ggccgagacg cugcauggaa ccgugacagu ugaagugcag     1560

uacgcgggca ccgacgggcc uugcaaaguc cccgcccaaa uggcugugga caugcagaca     1620

cugaccccug ugggaagacu caucacggcc aaucccguua uuacagaggg caccgaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggggacu cuuacaucgu caucggcgug     1740

ggagagaaaa agauuacaca ccacuggcau cgcuccggca gcaccaucgg gaaggcuuuu     1800

gaagccaccg ugcggggagc gaaaagaaug gccguccugg gcgauacagc uugggacuuc     1860

ggguccgugg gaggcguucu caacucucug gggaagggca uucaccagau cuucggagcc     1920

gcuuuuaaaa gccuguucgg cgggaugucc ugguuuucuc aaauucuuau cggaacgcuc     1980

cugauguggc ugggccucaa uaccaagaac gggagcaucu cccugaugug ucuugcccug     2040

ggaggcgugc ucauuuuccu gagcacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9023
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 3 opt

<400>  9023
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucagc      720

aauauggccg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacagcccca gagcggaagc cacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaagccag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucucuu auucccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaucccguua uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcagcggau ccacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcucugucg ggggagcucu caacagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaagaac ggcuccauca gccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gucuaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9024
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 3 opt

<400>  9024
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggaaacgcc uacuauaugu accuugaccg cuccgaugcg      120

ggcgaagcua uuucuuuccc cacaaccaug gggaugaaua agugcuauau ccagauuaug      180

gaccugggac acauguguga ugccacaaug agcuacgagu gcccuaugcu cgacgaaggc      240

guggagccgg augacgucga uuguuggugc aacaccacau ccaccugggu gguuuauggg      300

acgugucauc acaaaaaggg agaagccaga cggucucgca gagcugugac ccugcccagc      360

cauuccacac ggaaacuuca aacccgcucu cagacauggc uggagagcag agaguacacc      420

aagcaccuca uccgggucga aaauuggauu uuucgcaacc cuggcuucgc ccuggcugcc      480

gcggccaucg cuuggcugcu cggcuccagc acgucucaga aagugaucua ucugguuaug      540

auucuucuga ucgcccccgc uuacuccauu agaugcaucg gggugagcaa ucgggacuuu      600

gucgagggaa ugucuggcgg gacaugggug gauguggucc ucgaacaugg aggcuguguu      660

accgugaugg cccaggacaa gccuacagug gauauugagc uggucaccac cacagugucc      720

aacauggcgg agguucgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggccuac cuugacaagc agucugacac ccaguaugug      840

ugcaaacgga cacuggucga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgcuaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc uggaguacag aaucaugcuc ucuguucacg gaucccaaca cagcggcaug     1020

aucgugaaug acaccgggca ugaaacggau gagaaccggg ccaaggucga gauuacaccg     1080

aauagcccca gagcugaagc gacccuggga ggcuucggca gccuugggcu ggacugugag     1140

ccucgcaccg gacucgauuu uuccgaccug uauuaccuua caaugaacaa uaaacacugg     1200

cuggugcaua aggaaugguu ccacgauauc ccccucccuu ggcacgccgg cgcugacacc     1260

gggacacccc auuggaacaa uaaagaggcc cuggucgaau uuaaggaugc ccacgcuaaa     1320

cggcagacgg ugguugugcu gggaucucag gagggcgccg uccauaccgc gcucgcuggg     1380

gcccuggagg ccgaaaugga cggcgcuaag ggaagacuua gcuccggcca ccugaaaugc     1440

cgccucaaga uggauaagcu gcggcuuaaa ggggugagcu auucucugug uacagccgcu     1500

uucaccuuua caaagauucc ggccgagacc cuccauggaa cgguuaccgu ggaaguccag     1560

uacgcgggca cagacgggcc uugcaaagug cccgcccaaa uggcugugga caugcagacc     1620

cugacaccug uuggaagacu gaucaccgcc aaccccguca uuacggaguc cacagaaaac     1680

agcaagauga ugcucgagcu ggaucccccu uucggcgacu cuuacaucgu gaucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggcuuuu     1800

gaagccaccg ucagaggggc gaaacggaug gccgugcuug gagauaccgc uugggacuuc     1860

ggcuccguug ggggagcccu caauucucuc ggcaagggga uucaccagau cuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucugau cgggacacuu     1980

cucguguggc ugggacugaa cacgaagaau ggcagcaucu cccucaugug ucuggcccuu     2040

gggggagucc ugauuuuccu cagcaccgcu gugucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9025
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 3 opt

<400>  9025
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucagc      720

aauauggccg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgccaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucguuaacg acaccgggca ugaaacggau gagaaucggg cuaaggugga gauuacaccg     1080

aacagcccca gagcggaagc cacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaagccag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucucuu auucccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaucccguua uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcagcggau ccacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcucugucg ggggagcucu caacagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa uacgaagaac ggcuccauca gccugguuug ucuugcucuu     2040

gggggagugc ucauuuuccu gucuaccgcg gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9026
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 3 opt

<400>  9026
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcgaagcca uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aauaccacau cuaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggagccgca gagccguuac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcagcucu acgucccaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg ggguuaacaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugguuug ucuugcucuu     2040

gggggagugc ucauuuuccu gagcaccgcg gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9027
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 3 opt

<400>  9027
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggauccgcc uacuauaugu accuugaccg cucugaugcg      120

ggcgaagcua uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgucga uuguuggugc aauaccacau cuaccugggu gguuuauggg      300

acgugucauc acaaaaaggg agaagccaga cggagccgca gagcugugac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggucga aaacuggauu uuucgcaauc cuggcuucgc ccuggcugcc      480

gcggccaucg cuuggcuccu gggcagcucu acgucccaga aagugaucua ucuuguuaug      540

auucugcuca ucgcccccgc uuacagcauu agaugcaucg gggugucuaa ccgggacuuu      600

gucgagggaa uguccggcgg gacaugggug gauguggucc uggaacaugg aggcuguguu      660

accgugaugg cccaggacaa gccugcggug gauauugagc uugucacaac caccgugagc      720

aauauggccg agguucgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacacaagg ggaggcuuac cuggacaagc agagcgacac gcaguaugug      840

ugcaaacgga cccucgucga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga caugcgccaa auuugccugc agcaagaaaa ugaccgggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccguucacg gaagccaaca cucuggcaug     1020

aucgugaacg acacagggca ugaaaccgau gagaaucggg cuaaggucga gauuacgccg     1080

aacagcccca gagcggaagc cacacuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caaacacugg     1200

cucgugcaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggacacccc auuggaauaa caaagaggcc cuggucgaau uuaaggaugc ucacgccaaa     1320

cggcagaccg ugguugugcu cggaagccag gagggcgcgg uccauacggc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggugucuu auucccucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagaca cugcauggaa ccguuacggu ggaaguccag     1560

uacgccggca ccgacgggcc uugcaaagug cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug uuggaagacu caucacagcu aaucccguca uuaccgagag cacggaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu gaucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcagcggau ccaccaucgg caaggccuuu     1800

gaagcgacag ucagaggggc caaacggaug gcugugcugg gagauaccgc cugggacuuc     1860

ggcucuguug ggggagcucu caacagccug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacccuc     1980

cugguguggc ugggacucaa uacaaagaac ggcuccauca gccugacgug ucuugcucuu     2040

gggggagucc ucauuuuccu gucuaccgcg guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9028
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 3 opt

<400>  9028
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggauccgcc uacuauaugu accuugaccg cucugaugcg      120

ggcgaagcua uuagcuuccc cacaacccug gggaugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgucga uuguuggugc aauaccacau cuaccugggu gguuuauggg      300

acgugucauc acaaaaaggg agaagccaga cggagccgca gagcugugac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggucga aaacuggauu uuucgcaauc cuggcuucgc ccuggcugcc      480

gcggugaucg ccuggcuccu gggcagcucu acgucccaga aaguuaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacagcauu agaugcaucg gggucucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacaugggug gaugucguuc uggaacaugg aggcugugug      660

accgugaugg cucaggacaa gccuacaguc gauauugagc uugugaccac cacaguuagc      720

aauauggccg aggugcgcuc uuacugcuau gaagcgucca ucagcgacau ggccuccgau      780

ucuagauguc ccacgcaagg ggaggcuuac cuggacaagc agagcgacac ccaguauguc      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgccaa auuugcuugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucgucaacg acaccgggca ugaaacggau gagaaucggg ccaaggugga gauuacaccg     1080

aacagcccca gagccgaagc uacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcggg cgccgacacc     1260

gggacacccc auuggaauaa caaagaggcu cugguggaau uuaaggaugc ccacgccaaa     1320

cggcagacgg uugugguccu cggaagccag gagggcgcug ugcauaccgc ccuggcgggg     1380

gcucuugagg ccgaaaugga cggcgccaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa gggguuucuu auucccucug uacagcugcc     1500

uucaccuuua caaagauucc ggcugagacc cugcauggaa cggugaccgu cgaagugcag     1560

uacgccggca cagacgggcc uugcaaagug cccgcgcaaa uggccguuga caugcagacc     1620

cugacaccug ucggaagacu caucaccgcu aaucccguga uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu gaucgggguc     1740

ggcgagaaaa agauuaccca ccacuggcau cgcagcggau ccacaaucgg caaggccuuu     1800

gaagcuaccg ugagaggggc caaacggaug gcgguucugg gagauaccgc cugggacuuc     1860

ggcucugucg ggggagcucu caacagccug ggcaagggga uucaccagau cuucggcgcc     1920

gcuuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacacuc     1980

cugguguggc ugggacucaa uacgaagaac ggcuccauca gccugaccug ucuugcccuu     2040

gggggagugc ucauuuuccu gucuacagcc guuuccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9029
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 3 opt

<400>  9029
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggauccgcc uacuauaugu accuugaccg cucugaugcg      120

ggcgaagcua uuagcuuccc cacaacccug ggggugaaca agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgagu gcccuaugcu ggacgaaggc      240

gucgagccgg augacgugga uuguuggugc aauaccacau cuaccugggu uguguauggg      300

acgugucauc acaaaaaggg agaagccaga cggagccgca gagcugucac ccuucccucc      360

cauucuacac ggaaacugca aacccgcagc cagacauggc ucgaguccag agaguacacc      420

aagcaccuga uccgggugga aaacuggauu uuucgcaauc cuggcuucgc ccuggcugcc      480

gcggccaucg cuuggcuccu gggcagcucu acgucccaga aaguuaucua ucuugugaug      540

auucugcuca ucgcccccgc uuacagcauu agaugcaucg gggucucuaa ccgggacuuu      600

guggagggaa uguccggcgg gacaugggug gaugucguuc uggaacaugg aggcugugug      660

accgugaugg cccaggacaa gccuacaguc gauauugagc uugugaccac cacaguuagc      720

aauauggcgg aggugcgcuc uuacugcuau gaagccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaggccuac cuggacaagc agagcgacac ccaguauguc      840

ugcaaacgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgcuaa auuugccugc agcaagaaaa ugacagggaa gucuauucag      960

ccugaaaauc ucgaguacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucgucaacg acaccgggca ugaaacggau gagaaucggg ccaaggugga gauuacaccg     1080

aacagcccca gagcugaagc gacccuugga ggcuucggcu cccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga caaugaauaa caaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgccgg cgcugacacc     1260

gggacacccc auuggaauaa caaagaggcc cugguggaau uuaaggaugc ccacgcuaaa     1320

cggcagacgg uugugguccu cggaagccag gagggcgccg ugcauaccgc gcuggcuggg     1380

gcccuugagg ccgaaaugga cggcgcuaag ggaagacugu ccagcggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa gggguuucuu auucccucug uacagccgcu     1500

uucaccuuua caaagauucc ggccgagacc cugcauggaa cggugaccgu cgaagugcag     1560

uacgcgggca cagacgggcc uugcaaagug cccgcccaaa uggcuguuga caugcagacc     1620

cugacaccug ucggaagacu caucaccgcc aaucccguga uuacggagag cacagaaaac     1680

ucuaagauga ugcuggagcu ugaucccccu uucggcgacu ccuacaucgu gaucgggguc     1740

ggcgauaaaa agauuaccca ccacugguau cgcagcggau ccacaaucgg caaggcuuuu     1800

gaggccaccg ugagaggggc gaaacggaug gccguucugg gagacaccgc uugggauuuc     1860

ggcucugucg ggggagcccu caacagccug ggcaagggga uucaucagau cuucggcgcu     1920

gccuuuaaau cccuguucgg aggcaugucu ugguuuagcc aaauucuuau cgggacacuc     1980

cugguguggc ugggacucaa uacgaagaac ggcuccauca gccugaccug ucuugcccuu     2040

gggggagugc ucauuuuccu gucuacagcu guuuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9030
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 3 opt

<400>  9030
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcuguugagg ugaccagacg gggauccgcc uacuauaugu accuugaccg cucugaugcg      120

ggcaaggcua uuagcuuccc cacaacccug ggggugaaca aaugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug uccuacgaau gcccuaugcu ggacgagggc      240

gucgaaccgg augacgugga uuguuggugc aauaccacau cuaccugggu uguguauggg      300

acgugucauc acaagaaagg agaggccaga cggagccgca gagcugucac ccuucccucc      360

cauucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguacacc      420

aaacaccuga uccgggugga gaacuggauu uuucgcaauc cuggcuucgc ccuggcugcc      480

gcggccaucg cuuggcuccu gggcagcucu acgucccaga agguuaucua ucuugugaug      540

auucugcuca ucgcccccgc uuacagcauu agaugcaucg gggucucuaa ccgggacuuu      600

guggaaggaa uguccggcgg gacaugggug gaugucguuc uggagcaugg aggcugugug      660

accgugaugg cccaggacaa gccuacaguc gauauugaac uugugaccac cacaguuagc      720

aauauggcgg aggugcgcuc uuacugcuau gaggccucca ucagcgacau ggcuuccgau      780

ucuagauguc ccacgcaagg ggaagccuac cuggacaaac agagcgacac ccaguauguc      840

ugcaagcgga cacucgugga ucgcggaugg ggcaacggcu gugggcuguu cggaaaaggc      900

ucccugguua ccugcgcuaa guuugccugc agcaaaaaga ugacagggaa gucuauucag      960

ccugagaauc ucgaauacag aaucaugcug uccgugcacg gaagccaaca cucuggcaug     1020

aucgucaacg acauugggca ugagaccgau gaaaaucggg ccaaagugga gaucacgccg     1080

aacagcccca gagcugaggc gacacuugga ggcuucggcu cccuggggcu cgacugugaa     1140

ccucgcaccg gacuggauuu uucugaccuu uauuaccuga ccaugaauaa caagcacugg     1200

cucguccaua aagagugguu ccacgauauu ccccugccuu ggcacgccgg cgcugacaca     1260

gggacacccc auuggaauaa caaggaagcc cugguggagu uuaaagaugc ccacgcuaag     1320

cggcagacag uugugguccu cggaagccag gaaggcgccg ugcauacggc gcuggcuggg     1380

gcccuugagg ccgagaugga cggcgcuaaa ggaagacugu ccagcggcca ccucaagugc     1440

cgccugaaga uggauaaacu ucggcugaag gggguuucuu auucccucug uaccgccgcu     1500

uucacauuua ccaaaauccc ggccgaaaca cugcauggaa ccgugacggu cgaggugcag     1560

uacgcgggca ccgacgggcc uugcaaggug cccgcccaaa uggcuguuga caugcagaca     1620

cugaccccug ucggaagacu cauuacagcc aaucccguga ucaccgaaag cacggagaac     1680

ucuaaaauga ugcuggaacu ugaucccccu uucggcgacu ccuacaucgu gauugggguc     1740

ggcgauaaga agaucacaca ccacuggcau cgcagcggau ccaccauugg caaggcuuuu     1800

gaggccacag ugagaggggc gaaacggaug gccguucugg gagacaccgc uugggauuuc     1860

ggcucugucg ggggagcccu caacagccug ggcaaaggga uccaccagau uuucggcgcu     1920

gccuuuaagu cccuguucgg aggcaugucu ugguuuagcc aaauccuuau cgggacccuc     1980

cugguguggc ugggacucaa uaccaaaaac ggcuccauua gccugacgug ucuugcccuc     2040

gggggagugc ugaucuuccu gucuaccgcu guuuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9031
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 3 opt

<400>  9031
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga uuaaggugga gaacuggauc uuucggaauc ccggcuucac gcuggucgcc      480

guggcgaucg ccuggcuccu gggcagcucu acaucccaga agguuauuua ccuugugaug      540

auccugcuca uugcuccugc cuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gaccugggug gaugucguuc uggagcacgg aggcugugug      660

acagugaugg cucaggacaa acccaccguc gauaucgaac uugugaccac aacgguuagc      720

aauauggccg aggugcgguc uuacugcuac gaggcgucca uuagcgacau ggccuccgau      780

ucucgcuguc cuacccaagg ggaggcuuau cuggacaagc agagcgacac acaguacguc      840

ugcaaaagaa cccucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua caugcgccaa auuuaccugc agcaagaaga ugacggggaa aucuauccag      960

cccgaaaauc ucgaauaucg caucaugcug uccgugcacg gaagccaaca uucuggcaug     1020

auugucaacg acacagggca cgagaccgau gaaaauagag cuaaggugga ggucaccccu     1080

aacagcccgc gggccgaggc cacacuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaaccg gacuggauuu uucugaccuu uacuaucuga caaugaauaa caaacauugg     1200

cucgugcaca aggagugguu ccacgauauc ccucugcccu ggcaugcugg cgcggacacg     1260

gggaccccuc acuggaauaa caaagaagcc cugguugagu uuaaggaugc ucaugccaaa     1320

cgccagacag uggucgugcu cggaagccag gaaggcgccg uucacaccgc ucuggccggg     1380

gcgcuugagg cugagaugga cggcgccaag ggacggcugu ucuccggcca ucucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag ggggugagcu acucucucug uacagccgcu     1500

uuuaccuuca cgaagguccc cgccgaaacc cugcacggaa cagugaccgu ggagguucag     1560

uaugcuggca cagacgggcc gugcaaaguc ccugcccaaa uggcggugga caugcagacc     1620

cugacgcccg ugggacggcu cauuacagcc aauccuguca ucaccgaauc cacagagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacauugu gaucgggguu     1740

ggcgauaaga aaaucaccca ccauuggcac agauccggaa gcaccauugg caaggcuuuc     1800

gaggccacag ugcgcggggc uaaacggaug gccguccugg gagacacggc gugggauuuc     1860

ggcuccguug ggggaguguu uaacucucuc ggcaagggga uccaucagau uuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacccuu     1980

cucguguggc ugggacugaa uacaaagaac ggcagcaucu cccucaccug ucuggcccuu     2040

gggggaguca ugaucuuccu gagcacagcu gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9032
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 3 opt

<400>  9032
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaaaccug ggggugaaua aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuaccaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aacacaaccu cuacaugggu ggucuacggg      300

accugucacc auaagaaagg agaggccaga cggagccgca gagcugugac gcuuccgucc      360

cacucuaccc ggaagcugca aacacgcagc cagaccuggc ucgaauccag agaguauaca      420

aaacaucuga ucaagguuga gaauuggauc uuucggaacc ccggcuucgc ccuggcggcc      480

guggcuauug ccuggcuccu gggcagcucu accucccaga aggucaucua ccuugugaug      540

auucugcuca ucgcuccugc cuauagcauu cgcugcaucg ggguuucuaa uagagacuuu      600

guggaaggaa uguccggcgg gacguggguc gauguggugc uggagcacgg aggcuguguc      660

acaguuaugg cgcaggacaa acccaccgug gauauugaac uugugacaac caccgucagc      720

aacauggccg aggugcgguc uuacugcuac gaggcuucca ucagcgacau ggccuccgau      780

ucucgcuguc cuacacaagg ggaggcuuau cuggacaagc agagcgacac gcaguacguu      840

ugcaaaagaa cccucgugga ucggggaugg ggcaauggcu gugggcuguu cggaaagggc      900

ucccugguca caugcgccaa auuuaccugc agcaagaaga ugacagggaa aucuauccag      960

cccgaaaacc ucgaauaucg cauuaugcug uccgugcacg gaagccaaca uucuggcaug     1020

aucguuaaug acauugggca cgagaccgau gaaaacagag ccaaggugga ggucacgccu     1080

aauagcccgc gggcugaggc gacacuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaaccg gacuggauuu uucugaccuu uacuaucuga ccaugaacaa uaaacauugg     1200

cucgugcaca aggagugguu ccacgauauc ccucugcccu ggcaugccgg cgcugacaca     1260

gggaccccuc acuggaacaa uaaagaagcc cuggucgagu uuaaggaugc ccaugcuaaa     1320

cgccagacag ugguugugcu cggaagccag gaaggcgccg uccacacggc gcuggcuggg     1380

gcccuugagg ccgagaugga cggcgcuaag ggacggcugu ucuccggcca ucucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag ggggugagcu acucucucug uaccgccgcu     1500

uuuacauuca ccaagguucc cgccgaaaca cugcacggaa ccgugacggu cgaggugcag     1560

uaugcgggca ccgacgggcc gugcaaagug ccugcccaaa uggcuguuga caugcagaca     1620

cugacccccg ucggacggcu cauuacagcc aacccuguga ucaccgaauc cacggagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu gauugggguc     1740

ggcgauaaga aaaucacaca ccauuggcac agauccggaa gcaccauugg caaggcuuuc     1800

gaggccacag ugcgcggggc gaaacggaug gccguucugg gagacaccgc uugggauuuc     1860

ggcuccgucg ggggaguguu uaauucucuc ggcaaggggg ugcaucagau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucugau cgggacccuu     1980

cucguuuggc ugggacugaa cacaaagaau ggcagcaucu cccucacgug ucuggcccuu     2040

gggggaguga ugauuuuccu gagcaccgcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9033
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 3 opt

<400>  9033
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcugugac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga ucaagguuga gaacuggauc uuucggaauc ccggcuucgc ccugguggcg      480

gucgccauug cuuggcuccu gggcagcucu acgucccaga aggugaucua ccuuguuaug      540

auucugcuca ucgccccugc uuauagcauu cgcugcaucg gggugucuaa cagagacuuu      600

gucgaaggaa uguccggcgg gacaugggug gauguggucc uggagcacgg aggcuguguu      660

accgugaugg cccaggacaa acccacagug gauauugaac uugucaccac cacagugagc      720

aauauggcgg agguucgguc uuacugcuac gaggccucca ucagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacgug      840

ugcaaaagaa cacucgucga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguga ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg cauuaugcug uccguucacg gaagccaaca uucuggcaug     1020

aucgugaacg acauugggca cgagacggau gaaaauagag ccaaggucga ggugacaccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaaccg gacuggauuu uucugaccuu uacuaucuga caaugaauaa caaacauugg     1200

cucguccaca aggagugguu ccacgauauc ccucugcccu ggcaugcggg cgccgacacc     1260

gggacaccuc acuggaauaa caaagaagcu cugguggagu uuaaggaugc ccaugccaaa     1320

cgccagacgg uugugguccu cggaagccag gaaggcgcug ugcacaccgc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggacggcugu ucuccggcca ucucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag gggguuagcu acucucucug uacagcugcc     1500

uuuaccuuca caaaggugcc cgcugaaacc cugcacggaa cggucaccgu ggaggugcag     1560

uaugccggca cagacgggcc gugcaaaguu ccugcgcaaa uggccgucga caugcagacc     1620

cugacacccg ugggacggcu cauuaccgcu aauccuguga ucacggaauc cacagagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu cauuggggug     1740

ggcgauaaga aaaucaccca ccauuggcac agauccggaa gcacaauugg caaggccuuc     1800

gaggcuaccg uucgcggggc caaacggaug gcggugcugg gagacaccgc cugggauuuc     1860

ggcuccgucg ggggaguguu uaacucucuc ggcaagggga uccaucagau uuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccugau cgggacacuu     1980

cucguuuggc ugggacugaa uacgaagaac ggcagcauuu cccucaccug ucuggcucuu     2040

gggggaguga ugaucuuccu gagcacagcc gucucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9034
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 3 opt

<400>  9034
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaaaccug ggggugaaua aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuaccaug uccuaugaau gccccaugcu ggacgagggc      240

guugaacccg augacgugga uuguuggugc aacacaaccu cuacaugggu ggucuacggg      300

accugucacc auaagaaagg agaggccaga cggagccgca gagcugugac gcuuccgucc      360

cacucuaccc ggaagcugca aacacgcagc cagaccuggc ucgaauccag agaguauaca      420

aaacaucuga ucaagguuga gaauuggauc uuucggaacc ccggcuucgc ccuggcggcc      480

guggcuauug ccuggcuccu gggcagcucu accucccaga aggucaucua ccuugugaug      540

auucugcuca ucgcuccugc cuauagcauu cgcugcaucg ggguuucuaa uagagacuuu      600

guggaaggaa uguccggcgg gacguggguc gauguggugc uggagcacgg aggcuguguc      660

acaguuaugg cgcaggacaa acccaccgug gauauugaac uugugacaac caccgucagc      720

aacauggccg aggugcgguc uuacugcuac gaggcuucca ucagcgacau ggccuccgau      780

ucucgcuguc cuacacaagg ggaggcuuau cuggacaagc agagcgacac gcaguacguu      840

ugcaaaagaa cccucgugga ucggggaugg ggcaauggcu gugggcuguu cggaaagggc      900

ucccugguca caugcgccaa auuuaccugc agcaagaaga ugacagggaa aucuauccag      960

cccgaaaacc ucgaauaucg cauuaugcug uccgugcacg gaagccaaca uucuggcaug     1020

aucguuaaug acgagaacag agccaaggug gaagucaccc cuaauagccc gcgggcugag     1080

gcgacgcuug ggggauucgg cucccugggc cucgauugug agcccagaac agggcuggac     1140

uuuucugauc uuuacuaucu gaccaugaac aauaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaca uuccucugcc cuggcacgcc ggagcugaua ccggcacacc ucauuggaac     1260

aauaaagagg cccuggucga auuuaaggac gcccacgcua aacgccagac cgugguugug     1320

cucgggagcc aggagggagc cguccauaca gcgcuggcug gcgcccuuga agccgagaug     1380

gauggggcua agggccggcu guucuccgga caccucaagu gcagacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucucuc uguacggccg cuuuuaccuu cacaaagguu     1500

cccgccgaga cccugcaugg gacagugacc gucgaagugc aguaugcggg aacggauggc     1560

ccgugcaaag ugccugccca aauggcuguu gacaugcaga cccugacacc cgucgggcgg     1620

cucaucaccg ccaacccugu gauuacagag uccaccgaaa acagcaagau gaugcuggag     1680

cuugacccuc ccuuuggaga uucuuacauc gugaucggcg ucggggacaa gaaaauuacg     1740

caccacuggc auagauccgg cagcacaauc ggaaaggcuu ucgaagccac cgugcgcggc     1800

gcgaaacgga uggccguucu gggggauaca gcuugggacu ucggauccgu gggcgggguc     1860

uuuaauucuc ucggaaaggg cauucaccag aucuucgggg ccgcuuuuaa aagccuguuc     1920

ggcggaaugu ccugguuuuc ucaaauucug aucggcaccc uucucgugug gcuggggcug     1980

aacaccaaga auggaagcau cucccucaca ugucuggccc uuggcggggu uaugauuuuc     2040

cugagcacgg ccgugucugc uugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  9035
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 3 opt

<400>  9035
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga uuaaggugga gaacuggauc uuucggaauc ccggcuucgc ccuggucgcg      480

guggccaucg cuuggcuccu gggcagcucu acgucccaga agguuauuua ccuugugaug      540

auccugcuca uugccccugc uuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gacaugggug gaugucguuc uggagcacgg aggcugugug      660

accgugaugg cccaggacaa acccacaguc gauaucgaac uugugaccac cacaguuagc      720

aauauggcgg aggugcgguc uuacugcuac gaggccucca uuagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacguc      840

ugcaaaagaa cacucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg caucaugcug uccgugcacg gaagccaaca uucuggcaug     1020

auugucaacg acacggggca cgagacagau gaaaauagag ccaaggugga ggucaccccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaacag gacuggauuu uucugaccuu uacuaucuga ccaugaauaa caaacauugg     1200

cucgugcaca aggagugguu ccacgauauc ccucugcccu ggcaugcggg cgccgacaca     1260

gggacgccuc acuggaauaa caaagaagcu cugguugagu uuaaggaugc ccaugccaaa     1320

cgccagaccg uggucgugcu cggaagccag gaaggcgcug uucacacagc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggacggcugu ucuccggcca ucucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag ggggugagcu acucucucug uaccgcugcc     1500

uuuacauuca ccaagguccc cgcugaaacg cugcacggaa ccgugacagu ggagguucag     1560

uaugccggca ccgacgggcc gugcaaaguc ccugcgcaaa uggccgugga caugcagaca     1620

cugacccccg ugggacggcu cauuacggcu aauccuguca ucacagaauc caccgagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacauugu gaucgggguu     1740

ggcgauaaga aaaucacaca ccauuggcac agauccggaa gcaccauugg caaggccuuc     1800

gaggcuaccg ugcgcggggc caaacggaug gcgguccugg gagacacagc cugggauuuc     1860

ggcuccguug ggggaguguu uaacucucuc ggcaagggga uccaucagau uuucggcgcu     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccugau ugggacgcuu     1980

cucguguggc ugggacugaa uaccaagaac ggcagcaucu cccucacaug ucuggcucuu     2040

gggggaguca ugaucuuccu gagcaccgcc gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9036
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 3 opt

<400>  9036
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucaac acaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cauucuacac ggaagcugca gacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaccuga uuaaggugga gaacuggauc uuucggaauc ccggcuucgc ccuggucgcg      480

guggccaucg cuuggcuccu gggcagcucu acgucccaga agguuauuua ccuugugaug      540

auccugcuca uugccccugc uuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gacaugggug gaugucguuc uggagcaugg aggcugugug      660

accgugaugg cccaagacaa acccacaguc gauaucgaac uugugaccac cacaguuagc      720

aauauggcgg aggugcgguc uuacugcuac gaggccucca uuagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaggg ggaagccuau cuggacaagc agagcgacac ccaguacguc      840

ugcaaaagaa cacucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccaa      960

cccgagaauc ucgaauaucg caucaugcug uccgugcacg gaagccagca cucuggcaug     1020

auugucaacg acaucgggca ugagacggau gaaaauagag ccaaggugga ggucacaccu     1080

aacagcccgc gggccgaggc uacccuugga ggcuucggcu cccuggggcu cgacugugaa     1140

cccagaaccg gacuggauuu uucugaccuu uacuaucuga caaugaauaa caaacacugg     1200

cucgugcaua aggagugguu ccacgauauu ccucugcccu ggcacgcggg cgccgacacc     1260

gggacaccuc auuggaauaa caaagaagcu cugguugagu uuaaggaugc ccacgccaaa     1320

cgccagacgg uggucgugcu cggaagccag gaaggcgcug uucauaccgc ccuggcgggg     1380

gcucuugagg ccgagaugga cggcgccaag ggacggcugu ucuccggcca ccucaagugc     1440

agacugaaaa uggauaaacu ucgccugaag ggggugagcu acucucucug uacagcuguc     1500

ugcaccgccg cuaaggugcc cgccgaaaca cugcauggaa ccgugacggu ugagguccaa     1560

uaugcgggca ccgacgggcc guguaaagug ccugcccaga uggcugugga caugcagaca     1620

cugacccccg ucggacggcu caucacagcc aauccuguga uuaccgaauc cacggagaac     1680

agcaagauga ugcuggaacu ugauccuccc uuuggcgacu cuuacaucgu uaucggggug     1740

ggcgauaaga aaauuacaca ccacuggcau agauccggaa gcaccaucgg caaggcuuuc     1800

gaggccacag uccgcggggc gaaacggaug gccgugcugg gagacaccgc uugggauuuu     1860

ggcuccguug ggggaguguu caacucucuc ggcaagggga uucaccaaau cuucggcgcc     1920

gcuuuuaaaa gccuguucgg aggcaugucc ugguuuucuc agauucugau cgggacccuu     1980

cucgucuggc ugggacugaa uacaaagaac ggcagcaucu cccucacgug ccuggcccuu     2040

gggggaguga ugauuuuccu gagcaccgcc guuucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9037
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 3 opt

<400>  9037
gggagaaagc uuaccaugac cagcaucggc auuguggggc ugcuccugac aacggccaug       60

gcugccgaga ucaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca uuagcuucgc cacaacccug ggggucaaca aaugccacgu ucagaucaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guggaacccg augacgugga uuguuggugc aauaccacau cuaccugggu cguguacggg      300

acgugucacc auaagaaagg agaggccaga cggagccgca gagcuguuac ccuuccgucc      360

cacucuacac ggaagcugca aacccgcagc cagacauggc ucgaauccag agaguauacc      420

aaacaucuga uuaaggugga gaacuggauc uuucggaauc ccggcuucgc ccuggucgcg      480

guggccaucg cuuggcuccu gggcagcucu acgucccaga agguuauuua ccuugugaug      540

auccugcuca uugccccugc uuauagcauc cgcugcauug gggucucuaa cagagacuuu      600

guggaaggaa uguccggcgg gacaugggug gaugucguuc uggagcacgg aggcugugug      660

accgugaugg cccaggacaa acccacaguc gauaucgaac uugugaccac cacaguuagc      720

aauauggcgg aggugcgguc uuacugcuac gaggccucca uuagcgacau ggcuuccgau      780

ucucgcuguc cuacgcaagg ggaagccuau cuggacaagc agagcgacac ccaguacguc      840

ugcaaaagaa cacucgugga ucggggaugg ggcaacggcu gugggcuguu cggaaagggc      900

ucccugguua ccugcgcuaa auuuacaugc agcaagaaga ugaccgggaa aucuauccag      960

cccgagaauc ucgaauaucg caucaugcug uccgugcacg gaagccaaca uucuggcaug     1020

auugucaacg acgagaauag agccaaggug gaagucacgc cuaacagccc gcgggccgag     1080

gcuacacuug ggggauucgg cucccugggc cucgauugug agcccagaac cgggcuggac     1140

uuuucugauc uuuacuaucu gaccaugaau aacaaacacu ggcucgugca uaaggaaugg     1200

uuccacgaca ucccucugcc cuggcacgcg ggagccgaua caggcacccc ucauuggaau     1260

aacaaagagg cucugguuga auuuaaggac gcccacgcca aacgccagac aguggucgug     1320

cucgggagcc aggagggagc uguucauacg gcccuggcgg gcgcucuuga agccgagaug     1380

gauggggcca agggccggcu guucuccgga caccucaagu gcagacugaa aauggacaaa     1440

cuucgccuga agggcgugag cuacucucuc uguaccgcug ccuuuacauu caccaagguc     1500

cccgcugaga cacugcaugg gaccgugacg guggaaguuc aguaugccgg aaccgauggc     1560

ccgugcaaag ucccugcgca aauggccgug gacaugcaga cacugacccc cguggggcgg     1620

cucauuacag cuaauccugu caucaccgag uccacggaaa acagcaagau gaugcuggag     1680

cuugacccuc ccuuuggaga uucuuacauu gugaucggcg uuggggacaa gaaaaucaca     1740

caccacuggc auagauccgg cagcaccauu ggaaaggccu ucgaagcuac agugcgcggc     1800

gccaaacgga uggcgguccu gggggauacc gccugggacu ucggauccgu gggcgggguu     1860

uuuaacucuc ucggaaaggg cauccaccag auuuucgggg cugccuuuaa aagccuguuc     1920

ggcggaaugu ccugguuuuc ucaaauccug auuggcaccc uucucgugug gcuggggcug     1980

aauacaaaga acggaagcau cucccucacg ugucuggcuc uuggcggggu caugaucuuc     2040

cugagcaccg ccgugucugc cugaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  9038
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 3 opt

<400>  9038
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcuccugac aacggccaug       60

gcugccgaga uuaccagacg gggauccgcg uacuauaugu accuugaccg cucugaugcu      120

ggcaaggcca ucagcuucgc cacaacccug ggggugaaca aaugccacgu ccagauuaug      180

gaccucggac auauguguga ugcuacaaug uccuaugaau gccccaugcu ggacgagggc      240

guugaaccug augacgugga uuguuggugc aauaccacau cuaccugggu ggucuacggg      300

acgugucacc auaagaaagg agagaccaga cggagccgca gauccguguc ucuucgguau      360

cacuacacac gcaagcugca aaccagaagc cagacauggc ucgaaucccg ggaguauaaa      420

aagcaucuga ucaugguuga gaacuggauc uuucgcaauc cgggcuucgc cauugugagc      480

gucgcuauca ccuggcugau gggcucucuc acgucccaga aggugauuua ccugguuaug      540

aucgugcuua uuguccccgc cuacagcauc ucuugcauug ggguguccaa cagagaccug      600

guggaaggaa ugagcggcgg gacauggguc gauguugugc ucgagcacgg aggcugugug      660

accgaaaugg cgcaggacaa accuacaguc gauaucgagc uggugaccau gaccguuucu      720

aauauggccg aggugcgguc cuauugcuac gaagcuagcc uuuccgacau ggccucugcu      780

agccgcuguc ccacacaagg ggagccuucc cuggauaagc agagcgacac gcagucuguc      840

ugcaaaagaa cccucggaga ccggggcugg ggcaacgggu guggaaucuu uggcaagggg      900

ucccugguga caugcagcaa auuuaccugc uguaagaaga ugcccggaaa aucuauucag      960

ccugaaaauc uggaguaucg caucaugcuc ccgguucacg gcagccaaca uuccgggaug     1020

auugugaacg auaucggaca cgaaacagac gagaauagag ccaaggucga ggugaccccc     1080

aacucuccuc gggcggaagc cacgcugggc ggcuuuggga gccuuggacu ggauugcgag     1140

cccagaacag gccucgacuu cuccgaucug uacuaucuua ccaugaauaa caaacauugg     1200

cugguccaca aggaaugguu ucacgacauu ccucuccccu ggcaugcugg ggccgauacc     1260

ggaacaccgc acuggaauaa caaagaggcu cugguggagu ucaaggacgc ccaugccaaa     1320

cgccagaccg uugugguccu gggcagccag gaaggggcug ugcacacagc gcucgccggc     1380

gcucuggagg ccgaaaugga uggagccaag ggccggcuuu ucucugggca ucugaagugu     1440

agacucaaaa uggacaagcu gcgccuuaaa ggaguuuccu acagccugug cacggcugcc     1500

uucaccuuua caaaggugcc ugcggagacc cuccacggca cagucaccgu ggaagugcag     1560

ucugcuggga cggauggacc cuguaaaguu ccugcccaaa uggccgucga caugcagacc     1620

cugacacccg ugggccggcu gaucaccgcu aaucccguga ucacagaguc caccgaaaac     1680

agcaagauga ugcucgagcu ggacccuccg uucggggauu ccuauauugu caucggcgug     1740

ggagacaaga aaauuacgca ccauuggcac agaucuggca gcacaaucgg gaaggccuuu     1800

gaggcuaccg uucgcggagc caaacggaug gcggugcuug gcgauacagc cugggacuuc     1860

ggguccgucg gaggcguguu caacucucug gggaagggca uucaucagau cuuuggagcu     1920

gccuucaaaa gccucuuugg cgggaugucc ugguucagcc aaauccugau uggaacccug     1980

cuuguuuggc ucggccugaa uaccaagaac gggucuaucu cccugacaug ccucgcucug     2040

ggaggcguga ugauuuuucu uagcacggcc gucucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9039
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 3 opt

<400>  9039
gggagaaagc uuaccaugag cgugggcauc gucgggcugc uccugaccac agccauggcu       60

gccgagguua cgagacgggg auccgcguac uauauguacc uugaccgcaa cgaugcuggc      120

gaagccauuu cuuuccccac cacacugggg augaauaagu gcuauaucca gauuauggac      180

cucggacaca ugugugaugc caccaugagc uacgagugcc cuaugcugga cgaaggcgug      240

gagccggaug acguggauug uuggugcaac acaaccucca caugggucgu guaugggacc      300

ugucaucaca aaaagggaga agcuagacgg ucucgcagag ccguuacgcu ucccagccau      360

uccacccgga aacugcaaac acgcucucag accuggcucg agagcagaga guacacaaag      420

caccugaucc ggguggaaaa uuggauuuuu cgcaacccug gcuucgcucu ggccgcggcc      480

gcuaucgccu ggcuccuggg cuccagcacc ucucagaaag ucaucuaucu ugugaugauu      540

cugcucaucg cucccgccua cuccauuaga ugcaucgggg uuagcaaucg ggacuuugug      600

gagggaaugu cuggcgggac gugggucgau guggugcugg aacauggagg cugugucaca      660

guuauggcgc aggacaagcc uaccguggau auugagcuug ugacaaccac cgucuccaac      720

auggccgagg ugcgcagcua cugcuaugaa gccucuaucu ccgacauggc uagcgauucc      780

agauguccca cacaagggga ggcuuaccug gacaagcagu cugacacgca guauguuugc      840

aaacggaccc ucguggaucg cggauggggc aauggcugug ggcuguucgg aaagggcagc      900

cuggucacau gcgccaaauu ugccugcucc aagaaaauga ccggaaagag cauucagccu      960

gaaaaccucg aguacagaau caugcugucu gugcacgggu cccaacacag cggcaugauc     1020

guuaaugaca cagggcauga aaccgaugag aaccgggcua agguggagau uacgcccaau     1080

ucucccagag cggaagccac acuuggaggc uucggcagcc uggggcucga cugugagccu     1140

cgcaccggac uggauuuuuc cgaccuuuau uaccugacca ugaacaauaa acacuggcuc     1200

guccauaagg aaugguucca cgauaucccc cugccuuggc acgcuggcgc cgacacaggg     1260

accccgcauu ggaacaauaa agaggcccug guggaauuua aggaugcuca cgccaaacgg     1320

cagacagucg ugguucucgg aucucaggag ggcgcggugc auacggcucu ggccggggcc     1380

cuugaggcug aaauggacgg cgccaaggga agacugagcu ccggccaccu caaaugccgc     1440

cugaagaugg auaagcugcg gcugaaaggg gucagcuauu cucucuguac cgcugccuuc     1500

acauuuacca agauuccggc ggagacacug cauggaaccg ugacgguuga agugcaguac     1560

gccggcaccg acgggccuug caaagucccc gcucaaaugg ccguggacau gcagacacug     1620

accccugugg gaagacucau cacagcuaac cccguuauua ccgaguccac ggaaaacagc     1680

aagaugaugc uggagcuuga ucccccuuuc ggcgacucuu acaucgucau cggggugggc     1740

gagaaaaaga uuacacacca cuggcaucgc uccggaagca ccaucggcaa ggccuuugaa     1800

gcgacaguga gaggggccaa acggauggcu guccugggag auaccgccug ggacuucggc     1860

uccguugggg gagcucucaa uucucugggc aaggggauuc accagaucuu cggcgccgcc     1920

uuuaaaagcc uguucggagg cauguccugg uuuucucaaa uucuuaucgg gacccuccug     1980

auguggcugg gacucaacac aaagaauggc agcaucuccc ugaugugucu ugcucuuggg     2040

ggagugcuca uuuuccugag cacggcggug ucugccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  9040
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 3 opt

<400>  9040
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauauugagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca ucuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gauuacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauucc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu caucaccgcu aaccccguua uuacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuaccca ccacuggcau cgcuccggaa gcacaaucgg caaggccuuu     1800

gaagcgaccg ugagaggggc ccggcgcaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaaaggga uucaccagau cuucggcgcc     1920

gccuuuaaga gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacacuc     1980

cugauguggc ugggacucaa cacgaaaaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucauuuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9041
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 3 opt

<400>  9041
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggaug uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuauug ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auccugcuca uugcucccgc cuacuccauc agaugcauug ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accguuaugg cgcaggacaa gccuacagug gauaucgagc uugugaccac cacagucucc      720

aacauggccg aggugcgcag cuacugcuau gaagcuagca uuuccgacau ggccagcgau      780

uccagauguc ccacgcaagg ggaggcuuac cuggacaagc agucugacac ccaguauguu      840

ugcaaacgga cacucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugccugc uccaagaaaa ugacagggaa gucuauccag      960

ccugaaaacc ucgaguacag aauuaugcug ucugugcacg gaucccaaca cagcggcaug     1020

aucguuaaug acaccgggca ugaaacggau gagaaccggg cuaaggugga gaucacaccc     1080

aauucuccga gagcggaagc cacccuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga caaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauu ccccugccuu ggcacgcugg cgccgacacc     1260

gggacacccc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacgg ucgugguucu cggaucucag gagggcgcgg ugcauaccgc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uacagcugcc     1500

uucaccuuua caaagauccc ggcggagacc cugcauggaa cggugaccgu ugaagugcag     1560

uacgccggca cagacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ugggaagacu cauuaccgcu aaccccguua ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacauugu caucggggug     1740

ggcgagaaaa agaucaccca ccacuggcau cgcuccggaa gcacaauugg caaggccuuu     1800

gaagcgaccg ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccgucg ggggagcucu caauucucug ggcaagggga uccaccagau uuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauccuuau ugggacacuc     1980

cugauguggc ugggacucaa cacgaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggaguuc ucaucuuccu gagcaccgcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9042
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 3 opt

<400>  9042
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaugaccu ccacaugggu cguguauggg      300

accugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac gcuucccagc      360

cauuccaccc ggaaacugca aacacgcucu cagaccuggc ucgagagcag agaguacaca      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc accucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacguggguc gauguggugc uggaacaugg aggcuguguc      660

acaguuaugg cgcaggacaa gccuaccgug gauauugagc uugugacaac caccgucucc      720

aacauggccg aggugcgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacacaagg ggaggcuuac cuggacaagc agucugacac gcaguauguu      840

ugcaaacgga cccucgugga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguca caugcgccaa auuugccugc uccaagaaaa ugaccggaaa gagcauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucugugcacg ggucccaaca cagcggcaug     1020

aucguuaaug acacagggca ugaaaccgau gagaaccggg cuaaggugga gauuacgccc     1080

aauucuccca gagcggaagc cacacuugga ggcuucggca gccuggggcu cgacugugag     1140

ccucgcaccg gacuggauuu uuccgaccuu uauuaccuga ccaugaacaa uaaacacugg     1200

cucguccaua aggaaugguu ccacgauauc ccccugccuu ggcacgcugg cgccgacaca     1260

gggaccccgc auuggaacaa uaaagaggcc cugguggaau uuaaggaugc ucacgccaaa     1320

cggcagacag ucgugguucu cggaucucag gagggcgcgg ugcauacggc ucuggccggg     1380

gcccuugagg cugaaaugga cggcgccaag ggaagacuga gcuccggcca ccucaaaugc     1440

cgccugaaga uggauaagcu gcggcugaaa ggggucagcu auucucucug uaccgcugcc     1500

uucacauuua ccaagauucc ggcggagaca cugcauggaa ccgugacggu ugaagugcag     1560

uacgccggca ccgacgggcc uugcaaaguc cccgcucaaa uggccgugga caugcagaca     1620

cugaccccug ugggaagacu caucacagcu aaccccguua uuaccgaguc cacggaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacaucgu caucggggug     1740

ggcgagaaaa agauuacaca ccacuggcau cgcuccggaa gcaccaucgg caaggccuuu     1800

gaagcgacag ugagaggggc caaacggaug gcuguccugg gagauaccgc cugggacuuc     1860

ggcuccguug ggggagcucu caauucucug ggcaagggga uucaccagau cuucggcgcc     1920

gccuuuaaaa gccuguucgg aggcaugucc ugguuuucuc aaauucuuau cgggacccuc     1980

cugauguggc ugggacucaa cacaaagaau ggcagcaucu cccugaugug ucuugcucuu     2040

gggggagugc ucauuuuccu gagcacggcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9043
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 3 opt

<400>  9043
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccgagg uuaccagacg gggauccgcg uacuauaugu accuugaccg caacgaugcu      120

ggcgaagcca uuucuuuccc cacaacccug gggaugaaua agugcuauau ccagauuaug      180

gaccucggac acauguguga ugccacaaug agcuacgagu gcccuaugcu ggacgaaggc      240

guggagccgg augacgugga uuguuggugc aacaccacau ccaccugggu cguguauggg      300

acgugucauc acaaaaaggg agaagcuaga cggucucgca gagccguuac ccuucccagc      360

cauuccacac ggaaacugca aacccgcucu cagacauggc ucgagagcag agaguacacc      420

aagcaccuga uccgggugga aaauuggauu uuucgcaacc cuggcuucgc ucuggccgcg      480

gccgcuaucg ccuggcuccu gggcuccagc acgucucaga aagucaucua ucuugugaug      540

auucugcuca ucgcucccgc cuacuccauu agaugcaucg ggguuagcaa ucgggacuuu      600

guggagggaa ugucuggcgg gacauggguc gauguggugc uggaacaugg aggcuguguc      660

accgcgaugg cccaggacaa gccuacaguu gauauugagc uugugaccac cacagugucc      720

aacauggcug agguccgcag cuacugcuau gaagccucua ucuccgacau ggcuagcgau      780

uccagauguc ccacgcaagg ggaggccuac cuggacaagc agucugacac ccaguaugug      840

ugcaaacgga cacucguuga ucgcggaugg ggcaauggcu gugggcuguu cggaaagggc      900

agccugguga ccugcgccaa auuugcuugc uccaagaaaa ugacagggaa gucuauucag      960

ccugaaaacc ucgaguacag aaucaugcug ucuguccacg gaucccaaca cagcggcaug     1020

cuugugaaug acaccgggca ugaaacggau gagaaccggg cgaagguuga gaucacaccg     1080

aauagcccca gagccgaagc uacccuggga ggcuucggca gccucgggcu ggacugugag     1140

ccucgcaccg gacuugauuu uuccgaccug uauuaccuca caaugaacaa uaaacacugg     1200

cuggcccaua aggaaugguu ccacgauauu ccccugccuu ggcacgccgg cgcugccacc     1260

gggacacccc auuggaacaa uaaagaggcg cucguggaau uuaaggacgc ucacgccaaa     1320

cggcagacgg ucgugguccu gggaucucag gagggcgccg ugcauaccgc ucuugccggg     1380

gcucuggagg ccgaaaugga uggcgcgaag ggaagacuca gcuccggcca ccugaaaugc     1440

cgccuuaaga uggacaagcu gcggcucaaa gggguuagcu auucucugug uacagccgcu     1500

uucaccuuua caaagauccc ggccgagacc guggauggaa cggucaccgu ggaaggccag     1560

uacgggggaa cagacggccc uugcaaaguu cccgcucaaa uggccgugga caugcagacc     1620

cugacaccug ucgggagacu cauuaccgcg aaccccguga ucacggaguc cacagaaaac     1680

agcaagauga ugcuggagcu ugaucccccu uucggcgacu cuuacauugu gaucggaguu     1740

ggcgagaaaa agaucaccca ucacuggcac cgcuccggga gcacaauugg aaaggccuuu     1800

gaagcuaccg uccggggcgc caaaagaaug gcugugcugg gggauaccgc cugggacuuc     1860

ggauccgugg gcggggcccu caauucucug ggcaagggaa uccaucagau uaucggcgcu     1920

gcguucaaaa gccuguuugg gggaaugucc ugguucucuc aaauucuuau cggcacacuc     1980

cugauguggc uggggcucaa cacgaagaau ggaagcaucu cccugaugug ucuugcccug     2040

ggcggggucc ucauuuuucu gagcaccgcu gugucuggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9044
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 3 opt

<400>  9044
gggagaaagc uuaccauggg cgccgacgcu agcaucggga uugugucccu gcuccugacc       60

acagucaugg ccgcggagau cacgagacgg ggaucugcuu acuauaugua ccuugaucgc      120

agcgacgccg gcaaggccau uuccuucguu accacacugg gggugaacaa augccacgug      180

cagaucaugg aucucggaca uaugugugac gcuaccaugu cuuaugaaug ccccaugcug      240

gaugagggcg ucgaacccga cgauguggac uguuggugca auacaaccag cacauggguu      300

guguacggga ccugucacca uaagaaagga gaggccagac ggucccgcag agcugucacg      360

cuuccgucuc acagcacccg gaagcugcaa acacgcuccc agaccuggcu cgaaagcaga      420

gaguauacaa aacaucugau uaagguggag aacuggaucu uucggaaucc cggcuucgcc      480

cugguugcgg uggccaucgc uuggcuccug ggaucuucca ccagccagaa ggucauuuac      540

cuugugauga uccugcucau ugccccugcu uauucuaucc gcugcauugg gguguccaac      600

agagauuuug ucgaaggaau gagcggcggg acguggguug acguggugcu ggagcacgga      660

ggcuguguca cagugauggc ccaggauaaa cccaccguug acaucgaacu ugugacaacc      720

accgucucua auauggcgga ggugcggucc uacugcuacg aggccagcau uuccgauaug      780

gcuucugaca gccgcugucc uacacaaggg gaagccuauc uggacaagca guccgauacg      840

caguacguuu gcaaaagaac ccucguggac cggggauggg gcaacggcug ugggcuguuc      900

ggaaagggca gccuggucac augcgcuaaa uuuaccugcu cuaagaagau gacagggaaa      960

uccauccagc ccgagaaucu cgaauaucgc aucaugcuga gcgugcacgg aucucaacau     1020

agcggcauga uugucaacga ugagaauaga gccaaggugg aaguuacccc uaacuccccg     1080

cgggccgagg cuacgcuugg gggauucggc ucucugggcc ucgacuguga gcccagaaca     1140

gggcuggauu uuagcgaccu uuacuaucug accaugaaua acaaacacug gcucgugcau     1200

aaggaauggu uccacgauau cccucugccc uggcacuccg gagcggacac cgagacaccu     1260

cauuggaaua acaaagaagc ccuggucgag uuuaaggaug cucacgccaa acgccagacc     1320

gugguugugc ucggcagcca ggaaggggcc guccauacag cucuggccgg agcgcuugag     1380

gcugagaugg acggcgccaa ggggcggcug ucuuccggcc accucaagug cagacugaaa     1440

auggauaaac uucgccugaa gggagugagc uacucucucu guacggccgc uuucaccuuu     1500

acaaaggugc ccgccgaaac ccugcauggc acaguuaccg ucgaggugca guaugcuggg     1560

cgggacggac cgugcaaagu gccugcccaa auggcggucg acaugcagac gcugaccccc     1620

gugggcagac ucauuacagc caauccuguu aucaccgaau ccacagagaa cagcaagaug     1680

augcuggaac uugauccucc cuucggggac uccuacauug ugaucggagu cggcgauaag     1740

aaaaucaccc accacuggca ucgcucuggg agcauuaucg gcaaggcuuu ugaggccacg     1800

gugcggggag cuaaaagaau ggccguucug ggcgacacag cgugggauuu cggguccgug     1860

ggaggcgucu ucaacucucu cgggaagggc auucaccaga ucuuuggcgc cgcuuucaaa     1920

agccuguuug ggggcauguc cugguucagc caaauucuga ucggaacccu ucucgugugg     1980

cugggccuga auacaaagaa cgggucuauc ucccucaccu gucuggcccu uggaggcguu     2040

augauuuuuc ugagcaccgc ugugucugcc ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  9045
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 3 opt

<400>  9045
gggagaaagc uuaccauggg cgccgacacc agcaucggga uuaucggacu gcuccugaca       60

acggcuaugg ccgcggagau uaccagacgg ggcuccgcuu acuauaugua ccuugaucgc      120

ucugacgccg ggaaggccau cagcuucgcu acaaccaugg gagugaacaa augccacguc      180

cagauuaugg aucugggcca uaugugugac gccacaaugu ccuaugaaug ccccaugcuc      240

gaugaggggg uugaaccuga cgauguggac uguuggugca auaccacauc uaccugggug      300

gucuacggaa cgugucacca uaagaaaggc gaggcuagac cgagccggcg cgccgugacc      360

cugcccuccc acucuacaag aaaacuucaa acccggagcc agacauggcu ggaaucccgc      420

gaguauacca agcaucucau caagguugag aacuggaucc ugagaaaucc uggcuuuacg      480

guggcggccc uggcuauggc cuggcuccug gggaacagca caucucagcg ggucauuuac      540

cuugugauga uccugcucau ugcucccgcc uauuccaucc gcugcauugg aguuagcaau      600

agagauuucg uggaaggcau gucuggggga accugggucg acguggugcu ggagcacggc      660

ggguguguca caguuauggc gcaggauaag ccuaccgugg acaucgaacu ugugaccaca      720

acggucucca acauggccga ggugcggagc uacugcuacg aggcuucuau uuccgauaug      780

gccagcgacu cccgcugucc cacccaagga gaggcuuauc uggacaaaca gucugauaca      840

caguacguuu gcaagagaac ccucguggac cggggcuggg gcaaugggug uggacuguuu      900

ggcaaaggga gccuggucac augcgccaag uuuaccugcu ccaaaaagau gacgggaaag      960

agcauccagc cugaaaaccu cgaauaucgc aucaugcugu cugugcacgg cucccaacau     1020

agcgggauga uuguuaauga uaucggacac gagacagacg aaaacagagc caaaguggag     1080

gucaccccca auucuccccg ggcugaggcg acccuuggcg gcuucgggag ccugggacuc     1140

gauugugaac cuagaacagg ccuggacuuc uccgaucuuu acuaucugac caugaacaau     1200

aagcauuggc ucgugcacaa agagugguuu cacgacauuc cccugccuug gcaugccggg     1260

gcugauacag gaacgcccca cuggaacaau aaggaagccc uggucgaguu caaagacgcc     1320

caugcuaagc gccagaccgu gguugugcuc ggcucucagg aaggggccgu ccacacagcg     1380

cuggcuggcg cccuugaggc cgagauggau ggagcuaaag gccggcuguu uagcgggcau     1440

cucaagugca gaaucaagau ggacaaacug cgccuuaagg gaguguccua cagccugugu     1500

accgccgcuu ucacauuuuc uaaaguuccg gccgaaaccc uccacggcac ggugaccguc     1560

gaggugcagu augcggggcg ggacggaccu ugcaaggugc ccgcccaaau ggcuguugau     1620

augcagacac ugaccccugu cggcagacug auuacagcca accccaucau caccgaaucc     1680

acggagaaca gccgcaugau gcucgaacug gacccgccuu ucggggauuc uuacauugug     1740

aucggcgugg gagacaaaaa gauuacacac cauuggcacc gguccggcag caccaucggg     1800

aaggcuuuug aggccacagu cagaggagcg aaacgcaugg ccgugcuugg cgauaccgcu     1860

ugggacuucg gguccguugg aggcguguuc aauucucugg ggaagggcgu ccaucaggug     1920

uuuggagccg cuuucaaaag ccucuuuggc gggauguccu gguucucuca aauucugauc     1980

ggaacccugc uuguuuggcu cggccugaac acaaagaaug ggagcaucuc ccuggccugu     2040

cucgcccugg gaggcgcuau gauuuuucuu agcacggcgg ugucugccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  9046
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 3 opt

<400>  9046
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggauau uguccuugaa cacgggggau gugugacagu uauggcccaa      180

gacaaaccua cgguggauau cgagcugguc accacaaccg uguccaauau ggcggaaguu      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu gcaugggucu cagcacuccg gaaugauugu caacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guugaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu ggaguucaag gaugcucacg ccaagcggca gacagucgug      840

guucucggga gccaggaagg agcggugcau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auccccgcgg agacccuuca cgggacagug accguugaag ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauua cagccaaucc cgugaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucguuauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9047
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 3 opt

<400>  9047
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9048
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 3 opt

<400>  9048
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgg cuaugaaaca      540

gaugaggacc gggccaaggu ugaagugacc ccuaacagcc cccgcgcuga ggccacacuc      600

gggggauuug gcucucuggg ccuggauugc gagccuagaa ccgggcuuga cuucuccgau      660

cuguacuacc ucacgaugaa uaacaaacau uggcuggugc acaaggaaug guuucaugac      720

auuccccuuc cuuggcacgc gggagccgac acaggcaccc cgcacuggaa uaacaaagag      780

gcucuggucg aguucaagga ugcccaugcu aagcggcaga cagugguugu gcucgggagc      840

caggaaggag ccguccacac cgcgcuggcc ggcgcucugg aagccgagau ggacggggcu      900

aaaggacgcc ucuuuuccgg ccaucugaag uguagacuua aaauggauaa gcugcggcuc      960

aaaggcgugu cuuauagccu gugcaccgcc gccuucacau uuacgaaggu ucccgcugag     1020

acccuucacg ggacagugac cgucgaagug caauacgcgg gaacagacgg cccuuguaaa     1080

auccccgucc agauggccgu ggauaugcag acccugacgc cuguugggcg ccucauuaca     1140

gcuaaucccg ugaucaccga guccaccgaa aacagcaaga ugaugcugga gcuggacccg     1200

ccuuucggag auucuuauau ugucaucggc gugggggaca agaaaaucac acaccauugg     1260

cacagauccg gcagcaccau uggaaaggcc uuugaagcca caguucgggg cgcuaaacgc     1320

auggccgugc ucggggauac ggcgugggac uucggaucuu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  9049
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 3 opt

<400>  9049
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9050
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 3 opt

<400>  9050
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauaca      540

ggcuaugaaa ccgacgagaa ucgggccaag guggaaguga caccuaacag cccccgcgcu      600

gaggccaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacggggcuu      660

gacuucuccg aucuguacua ccucacaaug aauaacaaac auuggcuggu ccacaaggaa      720

ugguuucaug acauuccccu uccuuggcac gcgggagccg acaccggcac accgcacugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccaug cuaagcggca gaccguugug      840

guccucggga gccaggaagg agccgugcac accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggaaa gcucuuuucc ggccaucuga aaugucgccu uaagauggau      960

aaacugagac ucaagggcgu uucuuauagc cugugcacag ccgccuucac guuuaccaaa     1020

gugcccgcug agacacuuca cgggaccguc acaguggaag uccaauacgc gggaaccgac     1080

ggcccuugua agauccccgu gcagauggcc guugauaugc agacgcugac accugugggg     1140

cggcucauua ccgcuaaucc cgucaucacc gaguccacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuau auugugaucg gcguugggga caaaaagauc     1260

acccaccauu ggcaccgcuc cggcagcaca auuggaaaag ccuuugaagc cacggugaga     1320

ggcgcuaagc ggauggccgu ccucggggau accgcguggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9051
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 3 opt

<400>  9051
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugacagu uaacgauauc      540

ggcuaugaaa ccgacgagaa ucgggccaag guggaaguga caccuaacag cccccgcgcu      600

gaggccaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacggggcuu      660

gacuucuccg aucuguacua ccucacaaug aauaacaaac auuggcuggu ccacaaggaa      720

ugguuucaug acauuccccu uccuuggcac gcgggagccg acaccggcac accgcacugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccaug cuaagcggca gaccguugug      840

guccucggga gccaggaagg agccgugcac accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggaaa gcucuuuucc ggccaucuga aaugucgccu uaagauggau      960

aaacugagac ucaagggcgu uucuuauagc cugugcacag ccgccuucac guuuaccaaa     1020

gugcccgcug agacacuuca cgggaccguc acaguggaag uccaauacgc gggaaccgac     1080

ggcccuugua agauccccgu gcagauggcc guugauaugc agacgcugac accugugggg     1140

cggcucauua ccgcuaaucc cgucaucacc gaguccacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuau auugugaucg gcguugggga caaaaagauc     1260

acccaccauu ggcaccgcuc cggcagcaca auuggaaaag ccuuugaagc cacggugaga     1320

ggcgcuaagc ggauggccgu ccucggggau accgcguggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9052
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 3 opt

<400>  9052
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgaugaa      540

aaucgggcca agguggaggu gacaccuaac agcccccgcg cugaagccac ccugggcggg      600

uuuggaucuc ucggccugga cugcgagccu agaacaggcc uugauuucuc cgaccuguau      660

uaccucacca ugaauaacaa acauuggcug guccacaagg agugguuuca ugauauuccc      720

cuuccuuggc acgcgggggc cgacacggga acaccgcacu ggaauaacaa agaagcucug      780

guggaguuca aggacgccca ugcuaagcgg cagaccguug ugguccucgg cagccaggaa      840

ggggccgugc acacagcgcu ggccggagcu cuggaggccg aaauggaugg cgcuaaaggg      900

cgccucuuuu ccggacaucu gaaguguaga cuuaaaaugg acaagcugcg gcucaaaggc      960

guuucuuaca gccugugcac cgccgccuuc accuuuacaa aggugcccgc ugagacgcuu     1020

cacggcaccg ucacagugga gguccaauau gcggggaccg auggaccuug uaaagugccc     1080

gcccagaugg cuguugacau gcagacacug accccugugg gccgccucau cacggccaau     1140

cccgucauua cagaauccac cgagaacagc aagaugaugc uggaacugga uccgccuuuc     1200

ggggacucuu acaucgugau uggaguuggc gauaagaaaa ucacccacca uuggcacaga     1260

uccgggagca caaucggcaa ggccuuugag gcuaccgugc ggggagccaa acgcauggcg     1320

guccucggcg acacagcuug ggauuucggg ucuugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  9053
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 3 opt

<400>  9053
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgg cuaugaaaca      540

gaugagaacc gggccaaggu ugaagugacc ccuaauagcc cccgcgcuga ggccacacuc      600

gggggauuug gcucucuggg ccuggacugc gagccuagaa ccgggcuuga uuucuccgac      660

cuguacuacc ucacgaugaa caauaaacau uggcuggugc acaaggaaug guuucaugau      720

auuccccuuc cuuggcacgc gggagccgac acaggcaccc cgcacuggaa caauaaagag      780

gcucuggucg aguucaagga cgcccaugcu aagcggcaga cagugguugu gcucgggagc      840

caggaaggag ccguccacac cgcgcuggcc ggcgcucugg aagccgagau ggauggggcu      900

aaaggaaagc ucuuuuccgg ccaucugaaa ugucgccuua agauggacaa acugagacuc      960

aagggcgugu cuuauagccu gugcaccgcc gccuucacau uuacgaaagu ucccgcugag     1020

acccuucacg ggacagugac cgucgaagug caauacgcgg gaacagaugg cccuuguaag     1080

auccccgucc agauggccgu ggacaugcag acccugacgc cuguugggcg gcucauuaca     1140

gcuaaccccg ugaucaccga guccaccgaa aauagcaaga ugaugcugga gcuggauccg     1200

ccuuucggag acucuuauau ugucaucggc gugggggaua aaaagaucac acaccauugg     1260

caccgcuccg gcagcaccau uggaaaagcc uuugaagcca caguuagagg cgcuaagcgg     1320

auggccgugc ucggggacac ggcgugggau uucggaucuu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  9054
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 3 opt

<400>  9054
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu cccuuagaug cauuggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuggaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau cgagcucgug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcauuucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccugga caagcagucc gauacccagu augucugcaa acggacacuu      360

guggaccgcg gguggggaaa cggcuguggc cuguuuggga agggaagccu cgugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucuggag      480

uacagaauca ugcugagcgu ccaugggucu cagcacuccg gaaugauugu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuugauu gcgagccuag aaccgggcug      660

gacuucuccg aucucuauua ccugacgaug aauaacaaac acuggcuugu gcauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccucgu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcugggga gccaggaagg agcgguccau accgcccugg cuggcgcccu cgaagcugag      900

auggacgggg ccaaaggacg ccuguccucu ggccaccuua aguguagacu gaaaauggau      960

aagcuccggc ugaaaggcgu gagcuacucc cuuugcaccg ccgcuuuuac auucacgaag     1020

auccccgcgg agacccugca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccucac gccugugggg     1140

cgccugauua cagccaaucc cguuaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcucgacc cgccuuuugg agauuccuac auugugaucg gcgucgggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucugggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9055
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 3 opt

<400>  9055
gggagaaagc uuaccaugag caagaaagug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccag      180

gacaagccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

caaggcgaag ccuaccucga caaacagucc gauacccagu augucugcaa gcggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga aaggaagccu ggugaccugc      420

gcuaaguucg ccuguucuaa gaaaaugacg ggcaagucca uccagcccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu caacacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaaa guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaagc acuggcuggu gcauaaagaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaagg aggcccuggu cgaguucaag gaugcucacg ccaaacggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaagggacg ccucuccucu ggccaccuga aauguagacu uaagauggau      960

aaacugcggc ucaagggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaaa     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaguaugc cggaacagac     1080

ggcccuugua aggugcccgc ucaaauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga gaaaaagauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaagc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9056
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 3 opt

<400>  9056
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu gguauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9057
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 3 opt

<400>  9057
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggaaa gcucuccucu ggccaccuga aaugucgccu uaagauggau      960

aaacugagac ucaagggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaaa     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aggugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cggcucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga gaaaaagauu     1260

acacaucacu ggcaucgcag cggcucuacc aucggaaagg cuuucgaagc cacagugaga     1320

ggcgcgaaac ggauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9058
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 3 opt

<400>  9058
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaccc agacgcugac accugugggg     1140

cgccucauca ccgccaaucc cguuauuacc gagagcacag aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg cuuucgaggc cacggugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac accgccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9059
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 3 opt

<400>  9059
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaaauc      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu gcaugggucu cagcacuccg gaaugauugu caacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guugaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu ggaguucaag gaugcucacg ccaagcggca gacagucgug      840

guucucggga gccaggaagg agcggugcau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auccccgcgg agacccuuca cgggacagug accguugaag ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauua cagccaaucc cgugaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucguuauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9060
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 3 opt

<400>  9060
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgcgca gaauggcugu ucucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9061
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 3 opt

<400>  9061
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggucu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu gguuaccugc      420

gccaaauucg cuuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu gaacgauacc      540

ggccaugaaa cagacgagaa ucgggccaag guggaaauua ccccuaacag cccccgcgcu      600

gaggccacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu ccauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcgggagccg acacaggcac cccgcauugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccacg cuaagcggca gacaguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uagcuacucc cugugcaccg ccgccuuuac auucacgaag     1020

auucccgcug agacccuuca cgggacagug accgucgaag ugcaauaugc gggaacagac     1080

ggcccuugua aaguccccgc ccagauggcu guggauaugc agacccugac gccuguuggg     1140

cgccucauca cagccaaucc cgugauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg ccuucgaggc uacaguucgg     1320

ggcgccaaac gcauggcggu gcucggggac acggcuuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9062
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 3 opt

<400>  9062
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaucg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugauug gcgucgggga aaagaaaauc     1260

acccaucacu ggcauagaag cggcucuaca auuggaaagg cuuucgaggc caccgugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9063
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 3 opt

<400>  9063
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcauugggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9064
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 3 opt

<400>  9064
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cuugcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuggaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccca cggucgauau ugagcucgug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccuaca      300

cagggcgaag ccuaccugga caagcagucc gauacccagu augucugcaa acggacacuu      360

guggaccgcg gguggggaaa cggcuguggc cuguuuggga agggaagccu cgugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaaccgga gaaucuggag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua cccccaacag cccucgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuugauu gcgagcccag aaccgggcug      660

gacuucuccg aucucuauua ccugacgaug aauaacaaac acuggcuugu gcauaaggaa      720

ugguuucacg acaucccucu gcccuggcac gccggagcug acacaggcac cccucauugg      780

aauaacaaag aggcccucgu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcugggga gccaggaagg agcgguccau accgcccugg cuggcgcccu cgaagcugag      900

auggacgggg ccaaaggacg ccuguccucu ggccaccuua aguguagacu gaaaauggau      960

aagcuccggc ugaaaggcgu gagcuacucc cuuugcaccg ccgcuuuuac auucacgaag     1020

auuccggcgg agacccugca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggccccugua aagugccugc ucagauggcc gucgauaugc agacccucac gcccgugggg     1140

cgccugauca cagccaaucc uguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcucgauc ccccguuugg agacuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucugggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9065
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 3 opt

<400>  9065
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga cccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucagcucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gucuuacagc cugugcacag ccgcuuuuac guucaccaag     1020

auucccgcgg agacacuuca cgggaccguu acaguggaag uccaauaugc cggaaccgac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacgcugac accugugggg     1140

cgccucauca ccgccaaucc cguuauuacc gaguccacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauucuuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagauc cggcagcaca aucggaaagg cuuucgaggc cacggugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac accgccuggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9066
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 3 opt

<400>  9066
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcuggc ucagauggcc gucgauaugc agacccucac gcccgugggg     1140

cgccugauca cagccaaucc uguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcucgauc ccccguuugg agacuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucugggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9067
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 3 opt

<400>  9067
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaagcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaggcugag      900

auggaugggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggac      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg aaacccuuca cgggacaguu accguggagg uccaauaugc cggaacagau     1080

ggcccuugua aagugcccgc ucagauggcc gucgacaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gaaagcaccg agaacucuaa gaugaugcug     1200

gaacuggauc cgccuuuugg agacuccuac aucgugaucg gcgucgggga gaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggau acggccuggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9068
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 3 opt

<400>  9068
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcggccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacagggcuu      660

gacuucuccg aucuguauua ccucaccaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcuggagccg acacgggcac accgcauugg      780

aauaacaaag aggcucuggu cgaguucaag gaugcccacg cgaagcggca gaccgugguu      840

gugcucggga gccaggaagg agccguccau acagcucugg ccggcgcucu ggaagccgag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg cugcguuuac cuucacaaag     1020

auucccgccg agacgcuuca cgggaccguu acaguggaag uccaauaugc uggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauca cggcuaaucc cguuauuaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg ccuucgaggc gaccgugcgg     1320

ggcgcuaaac gcauggccgu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9069
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 3 opt

<400>  9069
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug uccaccacag uuucuaauau ggcggaagug      240

cgcagcuacu gcuaugaggc uaccaucucc gacaucgccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu ccaugggucu cagcacuccg gaaugauugu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagcggug      840

guucucggga gccaggaagg agccgugcau accgcucugg ccggcgcucu ggaagccgag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cagcuacucc cugugcaccg cugcguuuac auucacgaag     1020

auccccgccg agacccuuca cgggacagug accguugaag ugcaauaugc uggaacagac     1080

ggcccuugua aaguccccgc ccagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauua cagcuaaucc cgugaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucguuauug gcguggggga aaagaaaauc     1260

acacaucacu ggcauagaag cggcucuacc auuggaaagg ccuucgaggc gacaguccgg     1320

ggcgcuaaac gcauggccgu gcucggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9070
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 3 opt

<400>  9070
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucagcaag     1020

auucccgcgg agacgcuuca cgggaccguu acagaagagg ugcaauaugc cggaaccgac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacacugac cccugucggg     1140

cgccucauca cggccaaucc cgugauuaca gaaucuaccg agaacuccaa gaugaugcug     1200

gaacuggacc cgccuuuugg agauagcuac aucguuaucg gcguggggga gaagaaaauu     1260

acccaucacu ggcauagauc uggcagcaca aucggaaagg cuuucgaggc caccguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acagccuggg auuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9071
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 3 opt

<400>  9071
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaac ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccucac gccugucggg     1140

cgccugauca cagccaaucc cgugauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcucgacc cgccuuuugg agauuccuac aucguuaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcugggggac acggccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9072
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 3 opt

<400>  9072
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcauu ucuaaccggg acuucguuga ggggaugagc      120

ggaggcaccu ggguggaugu gguccuugaa cacgggggau gugugacagu uauggcccaa      180

gacaaaccua cgguggauau cgagcugguc accacaaccg uguccaauau ggcggaaguu      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uucaacccga gaaucucgag      480

uacagaauca ugcugagcgu gcaugggucu cagcacuccg gaaugauugu caacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guugaaauca ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcauucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucucug aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu ggaguucaag gaugcucacg ccaagcggca gacagucgug      840

guucucgggu cccaggaagg agcggugcau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucagcucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu cucuuacagc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacagug accguugaag ugcaauaugc cggaacagac     1080

ggcccuugua aaguccccgc ucagauggcc guggauaugc agacccugac gccugucggg     1140

cgccucauca cagccaaucc cgugaucacc gaguccaccg aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauucuuac auuguuaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagauc cggcagcacc aucggaaagg cuuucgaggc cacaguccgg     1320

ggcgcgaaac gcauggcugu gcucggggac acggccuggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9073
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 3 opt

<400>  9073
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ugaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uagcuacucc cugugcaccg ccgccuuuac auucacgaag     1020

auucccgcug agacccuuca cgggacagug accgucgaag ugcaauaugc gggaacagac     1080

ggcccuugua aaguccccgc ccagauggcu guggauaugc agacccugac gccuguuggg     1140

cgccucauca cagccaaucc cgugauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgucaucg gcguggggga aaagaaaauu     1260

acacaucacu ggcauagaag cggcucuacc aucggaaagg ccuucgaggc uacaguucgg     1320

ggcgccaaac gcauggcggu gcucggggac acggcuuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9074
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 3 opt

<400>  9074
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggagcugac     1080

ggcccuugua aagugcccgc ccagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauca cggcuaaucc cguuauuaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg ccuucgaggc gaccgugcgg     1320

ggcgcuaaac gcauggccgu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9075
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 3 opt

<400>  9075
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac acaggagccg acaccggcac accgcauugg      780

aauaacaaag aggcucuggu cgaguucaag gaugcccacg cuaagcggca gaccgugguu      840

gugcucggga gccaggaagg agccguccau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcacag ccgccuuuac guucaccaag     1020

auucccgcug agacacuuca cgggaccguu acaguggaag uccaauaugc gggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcu gucgauaugc agacgcugac accugugggg     1140

cgccucauca ccgccaaucc cguuauuacc gagggaacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg cgauucuuac aucgugaucg gggucggcga aaagaaaauu     1260

acccaucacu ggcauagauc cggaagcaca aucggcaagg ccuucgaggc uacggugcgc     1320

ggggccaaac ggauggcggu ucucggagac accgcuuggg auuuuggcuc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9076
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 3 opt

<400>  9076
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagc cauggcgcaa      180

gacaaaccua cgguggauau ugagcugguc accacaaccg uguccaauau ggcugaaguu      240

cgcucuuacu gcuaugaggc cagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgagg cuuaccucga caagcagucc gauacccagu augugugcaa acggacacug      360

gucgaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gccaaauucg cuuguucuaa gaagaugacg ggcaaaucca uccaacccga aaaucucgag      480

uacagaauua ugcugagcgu gcaugggucu cagcacuccg gaaugcuggu caacgauacc      540

ggccaugaaa cagacgagaa ucgggccaag guugaaauca ccccuaacag cccccgcgcg      600

gaggccacac ucgggggauu uggcucucug ggccuugauu gcgagccuag aaccgggcug      660

gacuucuccg aucucuauua ccugacgaug aauaacaaac acuggcuugc ucauaaggaa      720

ugguuucacg acauuccccu gccuuggcac gccggagcug ccacaggcac cccgcauugg      780

aauaacaaag aggcgcucgu ggaguucaag gacgcccacg cuaagcggca gacagugguc      840

gugcugggga gccaggaagg agccguucau accgcucugg ccggcgcccu cgaagcugag      900

auggaugggg cgaaaggacg ccuguccucu ggccaccuua aguguagacu gaaaauggac      960

aagcuccggc ugaaaggcgu gagcuacucc cuuugcaccg ccgcuuuuac auucacgaag     1020

auccccgccg agaccgucga ugggacagug accguugaag gacaauaugg cgggacagac     1080

ggaccuugua aagugcccgc ccagauggcu gucgauaugc agacccugac gccugugggc     1140

cgccucauua cagccaaucc cgucaucacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg ggauuccuac auugugaucg gcguuggaga aaagaaaauc     1260

acacaccauu ggcacagaag cggcucuacc auugggaagg cguucgaggc uacagugcgg     1320

ggagccaaac gcauggccgu ccucggcgac acggcuuggg auuuugggag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9077
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 3 opt

<400>  9077
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccug cggucgauau ugagcuggug acgaccacag uuuccaauau ggcugaagug      240

cgcucuuacu gcuaugaggc cagcaucucc gacauggccu cugauagcag auguccgacc      300

cagggcgagg cuuaccucga caagcagucc gauacacagu augucugcaa acggacccug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugacaugc      420

gccaaauucg cuuguucuaa gaagaugacc ggcaaaucca uccaacccga aaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacg      540

ggccaugaaa ccgacgagaa ucgggccaag guggaaauua caccuaacag cccccgcgcg      600

gaggccaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacagggcuu      660

gacuucuccg aucuguauua ccucaccaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcuggagccg acacgggcac accgcauugg      780

aauaacaaag aggcucuggu cgaguucaag gaugcccacg cgaagcggca gaccgugguu      840

gugcucggga gccaggaagg agccguccau acagcucugg ccggcgcucu ggaagccgag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg cugcguuuac cuucacaaag     1020

auucccgccg agacgcuuca cgggaccguu acaguggaag uccaauaugc uggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauca cggcuaaucc cguuauuaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg ccuucgaggc gaccgugcgg     1320

ggcgcuaaac gcauggccgu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9078
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 3 opt

<400>  9078
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu aacaaucggg acuucgugga ggggaugucu      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuagcaacau ggcggaagug      240

cgcuccuacu gcuaugaggc uucuaucagc gacauggccu ccgauucuag auguccgaca      300

cagggcgaag ccuaccucga caagcagagc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa uggcuguggc cuuuuuggga agggaucccu ggugaccugc      420

gcuaaauucg ccuguagcaa gaagaugacg ggcaaaucua uccaacccga gaaccucgag      480

uacagaauua ugcuguccgu ccaugggagc cagcacucug gaaugaucgu uaaugauacc      540

ggccaugaaa cagacgagaa ccgggcuaag guggaaauua ccccuaauag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucccug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucucug aucuguauua ccucacgaug aacaauaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aacaauaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucgggu cccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucagcucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gucuuacagc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaccc cguuauuacc gaguccaccg aaaauagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauucuuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcauagauc cggcagcacc aucggaaagg cuuucgaggc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggac acggccuggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9079
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 3 opt

<400>  9079
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcgccg cugcguuuac cuucacaaag     1020

auucccgccg agacgcuuca cgggaccguu acaguggaag uccaauaugc uggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauca cggcuaaucc cguuauuaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga aaagaaaauu     1260

acccaucacu ggcauagaag cggcucuaca aucggaaagg ccuucgaggc gaccgugcgg     1320

ggcgcuaaac gcauggccgu ucucggggac acagccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9080
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 3 opt

<400>  9080
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauauu      540

ggccaugaaa ccgacgagaa ucgggcuaag guggaaauca caccuaacag cccccgcgcc      600

gaggcgaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacagggcuu      660

gacuucuccg aucuguauua ccucaccaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gccggagcug acacgggcac accgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gaccgugguu      840

gugcucggga gccaggaagg agcgguccau acagcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac cuucacaaag     1020

auccccgcgg agacgcuuca cgggaccguu acaguggaag uccaauaugc cggaaccgac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacacugac cccugugggg     1140

cgccucauua cggccaaucc cguuaucaca gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugauug gcgucgggga caagaaaauc     1260

acccaucacu ggcauagaag cggcucuaca auuggaaagg cuuucgaagc caccgugcgg     1320

ggcgcgaaac gcauggcugu ucucggggau acagccuggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9081
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 3 opt

<400>  9081
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauucg ccuguucuaa gaagaugacg ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgggcuaag guggaaauua ccccuaacag cccccgcgcc      600

gaggcgacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcggca gacagugguu      840

gugcucggga gccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggacg ccucuccucu ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu gagcuacucc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

cgccucauca cagccaaucc cguuauuacc gagagcaccg aaaacucuaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauuccuac aucgugaucg gcgucgggga caagaaaauu     1260

acacaucacu gguauagaag cggcucuacc aucggaaagg cuuucgaagc cacagugcgg     1320

ggcgcgaaac gcauggcugu ucucggggau acggccuggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9082
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 3 opt

<400>  9082
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauaca      540

ggccaugaaa ccgacgagaa ucgggccaag guggaaguga caccuaacag cccccgcgcu      600

gaggccaccc ucgggggauu uggcucucug ggccuggauu gcgagccuag aacggggcuu      660

gacuucuccg aucuguauua ccucacaaug aauaacaaac acuggcuggu ccauaaggaa      720

ugguuucacg acauuccccu uccuuggcac gcgggagccg acaccggcac accgcauugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccacg cuaagcggca gaccguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuuuucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uucuuacagc cugugcacag ccgccuucac guuuaccaag     1020

gugcccgcug agacacuuca cgggaccguc acaguggaag uccaauaugc gggaaccgac     1080

ggcccuugua aagugcccgc ccagauggcu guugauaugc agacgcugac accugugggg     1140

cgccucauca ccgccaaucc cgucauuacc gaguccacag aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuac aucgugauug gcguugggga caagaaaauc     1260

acccaucacu ggcauagauc cggcagcaca aucggaaagg ccuuugaagc uacggugcgg     1320

ggcgccaaac gcauggcggu ccucggggau accgcuuggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9083
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 3 opt

<400>  9083
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauauu      540

ggccaugaaa cagacgagaa ucgggccaag guggaaguga ccccuaacag cccccgcgcu      600

gaggccacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu ccauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcgggagccg acacaggcac cccgcauugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccacg cuaagcggca gacaguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuuuucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uucuuacagc cugugcaccg ccgccuucac auuuacgaag     1020

gugcccgcug agacccuuca cgggacaguc accguggaag uccaauaugc gggaacagac     1080

ggcccuugua aagugcccgc ccagauggcu guugauaugc agacccugac gccugugggg     1140

cgccucauua cagccaaucc cgucaucacc gaguccaccg aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuac auugugaucg gcguugggga caagaaaauc     1260

acacaucacu ggcauagauc cggcagcacc auuggaaagg ccuuugaagc uacagugcgg     1320

ggcgccaaac gcauggcggu ccucggggau acggcuuggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9084
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 3 opt

<400>  9084
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauauu      540

ggccaugaaa cagacgagaa ucgggccaag guggaaguga ccccuaacag cccccgcgcu      600

gaggccacac ucgggggauu uggcucucug ggccuggauu gcgagccuag aaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaaac acuggcuggu ccauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gcgggagccg acacaggcac cccgcauugg      780

aauaacaaag aggcucuggu ggaguucaag gaugcccacg cuaagcggca gacaguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaaaggacg ccucuuuucc ggccaccuga aguguagacu uaaaauggau      960

aagcugcggc ucaaaggcgu uucuuacagc cugugcaccg ccguguguac agccgcuaag     1020

guccccgcgg agacgcuuca cgggaccgug acagucgaag ugcaauaugc cggaaccgac     1080

ggcccuugca aaguucccgc ucagauggcc guggauaugc agacacugac cccugucggg     1140

cgccucauua cggccaaucc cgugaucaca gaguccaccg aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuucgg agauucuuac auuguuaucg gcguggggga caagaaaauc     1260

acccaucacu ggcauagauc cggcagcaca auuggaaagg cuuuugaagc caccguccgg     1320

ggcgcgaaac gcauggcugu gcucggggau acagccuggg acuucggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9085
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 3 opt

<400>  9085
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauuagaug caucggcguu ucuaaccggg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

cgcucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagcag auguccgaca      300

cagggcgaag ccuaccucga caagcagucc gauacccagu augucugcaa acggacacug      360

guggaccgcg gguggggaaa cggcuguggc cuuuuuggga agggaagccu ggugaccugc      420

gcuaaauuca cguguucuaa gaagaugacc ggcaaaucca uccaacccga gaaucucgag      480

uacagaauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgaugaa      540

aaucgggcca agguggaggu gacaccuaac agcccccgcg cugaagccac ccugggcggg      600

uuuggaucuc ucggccugga cugcgagccu agaacaggcc uugauuucuc cgaccuguau      660

uaccucacca ugaauaacaa acauuggcug guccacaagg agugguuuca ugauauuccc      720

cuuccuuggc acagcggggc ggacacggaa acaccgcacu ggaauaacaa agaggcccug      780

guggaguuca aggacgcuca ugccaagcgg cagaccguug ugguccucgg aucccaggaa      840

ggcgcugugc acacagcccu ggcgggggcc cuggaagcug agauggaugg agccaaaggc      900

cgccucucua gcgggcaucu gaaguguaga cuuaaaaugg acaagcugcg gcucaaagga      960

guuuccuaca gccugugcac cgcugccuuu accuucacaa aggugcccgc cgagacgcuu     1020

cacggcaccg ucacagugga aguccaauau gcuggccgcg augggccuug uaaagugccc     1080

gcgcagaugg ccguugacau gcagacccug acaccugugg gaagacucau caccgcuaau     1140

cccgucauua cggagucuac agaaaacucc aagaugaugc uggagcugga uccgccuuuu     1200

ggcgacagcu acaucgugau ugggguugga gauaagaaaa ucacccacca uuggcaccgg     1260

ucuggcagca ucauugggaa ggccuucgaa gccaccgugc gcggcgcuaa aagaauggcc     1320

guccucggag acacagcgug ggauuuuggc uccugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  9086
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 3 opt

<400>  9086
gggagaaagc uuaccaugag ccagaaggug aucuaccugg ucaugauugu ucucaucgug       60

cccgccuauu ccauuucuug caucggcgug agcaacagag accuggucga ggggaugucc      120

ggaggcaccu ggguggaugu ugugcuugaa cacgggggau gugucacaga gauggcucaa      180

gacaaaccua cgguggauau ugaacugguu accaugacag ugucuaauau ggccgagguc      240

cggagcuacu gcuaugaagc gucccucucu gacauggcua gcgccucccg cuguccgacc      300

cagggcgagc ccagccugga uaagcagucu gacacacagu ccgugugcaa aagaacccuu      360

ggggaucggg gauggggcaa cggcuguggg aucuucggaa agggcagccu ggugacaugc      420

ucuaaauuua ccuguugcaa gaagaugccu gggaaaucca uccaacccga gaaucucgaa      480

uaccgcauua ugcugccugu ccauggaagc cagcacucug gcaugaucgu uaacgacauu      540

gggcaugaga cggaugaaaa uagagccaag guggagguga cccccaacuc cccucgggcu      600

gaggccacac ugggaggcuu cggcagccuc gggcuggacu gugaaccgcg caccggacuu      660

gauuuuuccg accuguauua ccucacaaug aauaacaaac acuggcuggu ccauaaggag      720

ugguuccacg auaucccccu uccuuggcac gcuggcgccg acaccgggac gccccauugg      780

aauaacaaag aagcgcuggu ggaguuuaag gacgcccacg cuaagagaca gacaguugug      840

guccucggau cucaggaagg cgccgugcau accgcucugg ccggggcgcu ggaggccgag      900

auggauggag cuaaaggccg ccucuucagc ggccaccuga agugccggcu uaaaauggac      960

aagcugagac ucaaaggggu uuccuauagc cuguguacag ccgcuuuuac cuucaccaag     1020

gugccugccg aaacacuuca cggaacgguc accguggagg uccaaucugc cggcacagau     1080

gggcccugca aagugccggc ucagauggcg guugacaugc agacccugac accuguggga     1140

cggcucauua ccgccaaucc cgucaucacg gaauccacag agaacagcaa gaugaugcug     1200

gaacuggauc cucccuuugg cgacucuuac auugugaucg ggguuggcga uaagaaaauc     1260

acccaucacu ggcaucgcag cggauccacc auuggcaagg cuuucgaggc cacagugaga     1320

ggggccaaac ggauggcugu ccucggagac accgccuggg auuuuggcuc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9087
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 3 opt

<400>  9087
gggagaaagc uuaccaugag acagaaggug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuaua gcauucggug caucggcguu uccaaccgcg acuucgugga ggggaugucu      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaaaccua cggucgauau ugagcuggug accacaaccg uuagcaauau ggcggaagug      240

agauccuacu gcuaugaggc uucuaucagc gacauggccu ccgauucucg guguccgaca      300

cagggcgaag ccuaccucga caagcagagc gauacccagu augucugcaa acgcacacug      360

guggacagag gguggggaaa cggcuguggc cuuuuuggga agggaucccu ggugaccugc      420

gcuaaauucg ccuguagcaa gaagaugacg ggcaaaucua uccaacccga gaaucucgag      480

uaccggauua ugcuguccgu ccaugggagc cagcacucug gaaugaucgu uaacgauacc      540

ggccaugaaa cagacgagaa ucgcgcuaag guggaaauua ccccuaacuc ccccagagcc      600

gaggcgacac ucgggggauu uggcagccug ggccuggauu gcgagccucg gaccgggcuu      660

gacuucucug aucuguauua ccucacgaug aauaacaaac acuggcuggu gcauaaggaa      720

ugguuucacg acaucccccu uccuuggcac gccggagcug acacaggcac cccgcauugg      780

aauaacaaag aggcccuggu cgaguucaag gaugcucacg ccaagcgcca gacagugguu      840

gugcucgggu cccaggaagg agcgguccau accgcccugg cuggcgcccu ggaagcugag      900

auggacgggg ccaaaggaag acucagcucc ggccaccuga agugucggcu uaaaauggau      960

aagcugcgcc ucaaaggcgu gucuuacagc cugugcaccg ccgcuuuuac auucacgaag     1020

auucccgcgg agacccuuca cgggacaguu accguggaag uccaauaugc cggaacagac     1080

ggcccuugua aagugcccgc ucagauggcc gucgauaugc agacccugac gccugugggg     1140

agacucauca cagccaaucc cguuauuacc gaguccaccg aaaacagcaa gaugaugcug     1200

gagcuggacc cgccuuuugg agauucuuac aucgugaucg gcgucgggga aaagaaaauu     1260

acacaucacu ggcaucgguc cggcagcacc aucggaaagg cuuucgaggc cacagugcgc     1320

ggcgcgaaaa gaauggcugu ucucggggac acggccuggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9088
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 3 opt

<400>  9088
gggagaaagc uuaccaugag ccagagagug aucuaccugg ucaugauucu ccugaucgcc       60

cccgcuuauu ccauucggug caucggcguu ucuaaccgcg acuucgugga ggggaugagc      120

ggaggcaccu ggguggaugu cgugcuugaa cacgggggau guguuacagu gauggcccaa      180

gacaagccua cggucgauau ugagcuggug accacaaccg uuuccaauau ggcggaagug      240

agaucuuacu gcuaugaggc uagcaucucc gacauggccu cugauagccg guguccgaca      300

cagggcgaag ccuaccucga caaacagucc gauacccagu augucugcaa gcgcacacug      360

guggacagag gguggggaaa cggcuguggc cuuuuuggga aaggaagccu ggugaccugc      420

gcuaaguuca cguguucuaa aaagaugacc ggcaagucca uccaacccga gaaucucgag      480

uaccggauua ugcugagcgu ccaugggucu cagcacuccg gaaugaucgu uaacgauauu      540

ggccaugaaa cagacgagaa ucgcgccaaa guggaaguga ccccuaacag ccccagagcu      600

gaggccacac ucgggggauu uggcucucug ggccuggauu gcgagccucg gaccgggcuu      660

gacuucuccg aucuguauua ccucacgaug aauaacaagc acuggcuggu ccauaaagaa      720

ugguuucacg acaucccccu uccuuggcac gcgggagccg acacaggcac cccgcauugg      780

aauaacaagg aggcucuggu ggaguucaaa gaugcccacg cuaagcgcca gacaguugug      840

guccucggga gccaggaagg agccgugcau accgcgcugg ccggcgcucu ggaagccgag      900

auggacgggg cuaagggaag acucuuuucc ggccaccuga aaugucggau uaagauggau      960

aaacuucgcc ugaagggcgu uucuuacagc cucugcaccg ccgccuucac auuuuccaaa     1020

gugcccgcug agacgcugca cgggaccguc acaguggaag uccaauaugc gggaagagac     1080

ggcccuugua aggugcccgc ccagauggcu guugauaugc agacccuuac accugugggg     1140

cggcugauca ccgccaaucc cauuaucacg gagagcacag aaaacucucg caugaugcuc     1200

gagcuggacc cgccuuucgg agauuccuac aucgucauug gcguggggga caaaaagauc     1260

acccaucacu ggcauagaag cggcucuacc auuggaaagg ccuuugaagc uacaguucgg     1320

ggcgccaaaa gaauggcggu gcugggggau accgcuuggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9089
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  9089
gggagaaagc uuaccaugag gauaauaaau gccagaaagg aaaaaaaaag aagaggagcu       60

gauacaagug uaggaauagu aggauuauua uugacaacag caauggcagc ugaaguaacg      120

agaagagguu cagcauauua uauguacuua gacaggaaug augcagguga agcaauauca      180

uucccaacaa cacuaggaau gaauaaaugu uauauacaaa uuauggaccu cggacauaug      240

ugugaugcua cgaugucaua ugaaugcccu augcuggacg agggaguuga accagaugau      300

guugauuguu ggugcaacac aacaaguaca ugggucguau auggaacaug ucaucauaaa      360

aaaggagaag cacgaaggag uagaagagcu guaacauuac caucacauuc aacgcgaaag      420

cuacaaacgc gaucacaaac augguuagaa agcagagaau acaccaaaca ucuaauuaga      480

guagagaauu ggauuuuuag aaauccagga uuugcacugg cggccgcugc aauugcaugg      540

cuauuaggaa guucaaccag ucaaaaagua auauaccuag uaaugauauu acuaauagcg      600

ccagcuuaua gcauaagaug cauaggaguu aguaaccgag auuuugucga gggaauguca      660

ggugguacuu ggguagacau agugcuagaa caugguggau guguaacagu uauggcacag      720

gacaaaccaa caguagacau ugaacucgua acaacaacug uaaguaauau ggcagaagua      780

cggucauacu guuaugaagc uaguauauca gauauggcaa gcgacagcag augcccaacu      840

caaggggaag cauacuuaga uaaacaaagu gauacucagu auguauguaa acgaacauua      900

guagacagag gauggggaaa cggaugugga cuuuucggua aagguagccu agugacaugu      960

gcaaaauuug cuuguaguaa aaaaaugacu ggaaaaucaa uacaacccga aaaucuagaa     1020

uauagaauaa uguugucagu ccacggauca cagcauucug guaugauugu aaaugauacu     1080

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccaaacag uccaagggca     1140

gaagcaacau uaggugguuu uggaucacua ggguuagauu gugaaccaag gacgggauua     1200

gauuuuagcg acuuauacua uuuaaccaug aacaauaaac acugguuagu acacaaagaa     1260

ugguuucacg auauaccguu gccguggcau gcgggagcug auacaggcac accacauugg     1320

aauaauaaag aagcccuagu agaauucaaa gacgcacacg cgaaaagaca aacaguagua     1380

guauuagguu cgcaagaagg cgcagugcau accgcauugg caggugcauu ggaagcagaa     1440

auggauggag caaaaggaag guuaaguucu ggacauuuga aauguagauu gaaaauggau     1500

aaacuaagac uaaaaggagu gagcuauuca uuauguaccg cagcauuuac auucacaaaa     1560

auuccagcag aaacguuaca cggaacaguu acgguagaag uacaauaugc agggacagau     1620

ggccccugua aaguaccagc ucaaauggcg guugauaugc aaacccuaac cccaguggga     1680

agauuaauua cagcuaaccc aguaauaaca gaaaguaccg aaaauagcaa aaugaugcuu     1740

gaacuagacc caccauuugg agauucauac auaguaauag gaguuggaga aaaaaagaua     1800

acacaucauu ggcauaggag uggaaguacu aucggaaaag cguuugaagc gacaguuaga     1860

ggagcaaaaa gaauggcugu guuaggggau acagcauggg auuuuggaag cgucggagga     1920

gccuuaaauu cuuuaggaaa aggaauucau caaauauuug gggccgcauu caaaagucua     1980

uuugguggaa ugucaugguu uucacaaaua uuaauaggaa cauuguuaau guggcuagga     2040

uuaaacacua aaaacggaag cauaucacua augugucuag cuuugggugg aguacuaaua     2100

uuucuaucca cugcuguauc agcagacgug ggguguagcg uggacuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9090
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 3 opt

<400>  9090
gggagaaagc uuaccaugag aauaaucaau gcgaggaaag aaaagaaaag aagaggagcc       60

gacacuagcg ucgguauagu agguuuauua uuaacaacug ccauggccgc cgaaguuaca      120

agaagagguu cagcauacua cauguauuua gauagaaaug augcaggaga agcuauuucc      180

uuuccuacaa cucuuggaau gaauaaaugu uauauccaaa uuauggaucu aggacauacg      240

ugcgaugcaa caaugagcua cgaaugucca auguuagaug aaggaguaga accagaugac      300

guugauugcu gguguaacac aacuucaaca uggguaguuu auggcacaug ucaucauaag      360

aaaggagaag caagaagaag uagacgugca guaacauuac caagucauuc aacuagaaaa      420

cuacaaacua ggagccaaac auggcuugaa aguagagagu auacaaaaca ccuuauuaga      480

guagaaaauu ggauauuuag gaauccagga uucgcguuag cagcagcugc aauagcaugg      540

uugcuaggaa gcucaacaag ucaaaaagua auauauuuag uaaugauacu ucuaauagca      600

ccagcauauu caauaagaug uaucggagua ucaaaccgag auuuugucga aggaaugucu      660

ggugguacau ggguagacgu aguauuagag cacggaggau guguaacagu aauggcacaa      720

gauaaaccaa caguggauau agaauugguc acaacaaccg uaucaaauau ggcugaaguc      780

agaagcuacu gcuaugaagc aucgauaagu gauauggcga gugacucuag augcccaacc      840

caaggagaag cauacuuaga uaaacaauca gacacacaau acguauguaa aagaacuuua      900

guagauagag guuggggcaa cggaugugga cucuuuggaa aaggaucgcu aguuacaugu      960

gcaaaauuug ccuguucaaa gaaaaugacg ggaaaaucaa uacagccaga aaauuuagaa     1020

uauagaauaa uguugagugu acauggaucc caacauucag gaaugauagu aaaugauaca     1080

ggacaugaaa cugacgaaaa uagagcaaaa guugaaauaa caccaaauuc accacgugca     1140

gaggcgacac ucggugguuu cgggucauug ggucuugauu gugaaccaag aacaggauua     1200

gauuuuucag auuuauauua cuuaacaaug aacaauaaac acugguuagu acauaaagaa     1260

ugguuucaug auauaccguu accauggcau gcaggagcag auacaggcac accacauugg     1320

aauaauaaag aagcauuagu ugaguucaaa gaugcgcacg cuaaaagaca aacaguuguc     1380

guauuaggua gucaagaagg agcaguacau acagcgcuag cuggagcacu agaagcugaa     1440

auggauggag caaaaggaag acuaaguagu ggucauuuaa aauguagauu aaaaauggac     1500

aaacuuaggu uaaaaggagu uaguuacuca uuauguacug cggcuuuuac auuuacaaaa     1560

auaccugcag aaacauuaca cgguacaguu acagucgaag uacaauaugc ugguacagac     1620

ggaccuugua aaguaccagc ucaaauggca guagauaugc aaacacuaac accaguugga     1680

cgccuaauaa ccgcaaaucc uguuauaaca gaaucuacgg aaaacucaaa aaugauguua     1740

gaauuagauc ccccauuugg ggauucauau auaguaauug ggguaggaga aaagaaaaua     1800

acacaucacu ggcacagaag uggaaguaca auaggaaaag cauucgaagc aacaguaaga     1860

ggagcaaaaa gaauggcagu ccuaggggac acugcauggg auuucggaag cguaggaggg     1920

gcuuuaaaua gucucgggaa aggaauccau caaaucuuug gagcagcguu uaaaucacua     1980

uuuggaggaa ugucaugguu uucacaaaua uuaauaggua cuuuacuaau gugguuagga     2040

cuaaaugcua aaaacggcuc aauuucauua auguguuuag cauuaggagg aguuuugaua     2100

uucuuaucaa cagcaguuag cgcugacguu ggauguagug uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9091
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 3 opt

<400>  9091
gggagaaagc uuaccaugcg aauaauaaac gcgagaaaag aacguaaacg ucgaggagca       60

gacacaagca uaggcauaau aggauuacug cuaacaaccg caauggcagc cgaaauaaca      120

agaagaggau cagccuauua uauguaucua gauagaagug augcagguaa agccauauca      180

uuugcaacca cguuaggagu aaauaaaugc cauguacaaa uaauggaccu uggacauaug      240

ugcgaugcaa caaugucuua ugaauguccu augcuagaug aaggaguaga accagaugac      300

guugauuguu gguguaauac aacuuccaca uggguagucu acggaacaug ccaucacaaa      360

aaaggugaag cuaggagguc aagacgagcc guaacauuac caucucauuc aacaagaaaa      420

uuacaaacaa gaucacaaac auggcuagaa ucaagggaau auacaaaaca ucuuauaaaa      480

guagaaaauu ggauuuucag aaauccuggu uuugcacuag uagcaguagc aauagccugg      540

cucuuaggua gcaguaccag ccaaaaagua auauaucucg ucaugauucu auuaauagcu      600

ccagccuaca gcauaagaug uauuggagua ucaaauagag auuuuguaga agguaugucu      660

ggaggaacau ggguugaugu aguuuuagaa caugggggau guguaacagu aauggcacaa      720

gauaaaccaa caguggacau ugaauuaguc accacaacug uaucuaauau ggcagaggua      780

cgaucauauu guuaugaagc aagcauaagc gauauggcga gugauucaag augccccaca      840

caaggagaag cuuauuuaga uaaacaaagu gauacacagu auguaugcaa aagaacccua      900

guggauagag guugggguaa ugguugcgga uuauuuggaa aaggaagucu aguaacaugu      960

gcaaaauuca cuuguucaaa aaaaaugaca gggaaaagua uacaaccaga aaauuuagaa     1020

uauagaauaa uguuaagcgu acaugguuca caacauucgg gaaugauagg auaugaaaca     1080

gaugaagaua gggccaaagu agaaguaaca ccaaacaguc caagagcaga agccacacuu     1140

ggugguuucg gaucucucgg auuagacugu gaaccucgaa cagggcuuga cuuuucagau     1200

cuauacuauu uaacaaugaa uaauaagcau uggcuaguuc acaaagaaug guuucacgau     1260

auuccgcuuc cauggcaugc gggagcagau acaggaacuc cacauuggaa uaauaaagaa     1320

gcacuaguug aauucaaaga ugcacaugca aaaagacaaa cagucguagu uuuaggauca     1380

caagaaggag caguacauac agcacuagcc ggagcuuuag aagcagaaau ggauggugca     1440

aaaggacgac uauuuagcgg acaccuaaaa ugucgucuaa aaauggauaa acuuagauua     1500

aaaggaguau cguacucguu auguacagca gcauuuacau uuacuaaagu gcccgcagaa     1560

acacuacaug gaacagugac uguugaagua caauaugcag gaacagaugg cccauguaaa     1620

auuccuguuc aaauggcagu ugauaugcaa acauuaacuc caguuggaag acuaauaaca     1680

gcaaacccug uuauaacaga aucaacugaa aauaguaaaa ugauguugga acuggaccca     1740

ccauucggag auaguuacau aguaauaggu guaggagaua agaaaauaac acaucauugg     1800

cacagaucag guaguaccau ugguaaagca uucgaagcaa caguuagagg agcaaaaaga     1860

auggcaguuc ucggagauac agcaugggau uuugguagug ugggaggugu uuuuaacagu     1920

uuagguaaag gaauacauca aaucuuuggu gcagcuuuua aaagccuauu uggaggaaug     1980

ucaugguucu cucaaauucu aauagguaca cuauuaguuu ggcuaggacu uaauacgaaa     2040

aaugggucua uuaguuuaac uuguuuagca cucggaggag uaaugauauu cuuaaguaca     2100

gcaguauccg cugacgucgg uuguucagua gauuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  9092
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 3 opt

<400>  9092
gggagaaagc uuaccaugag aauaauaaau gcaaggaaag aaaagaaaag aagaggagca       60

gacacaagcg uagguauagu aggguuauua uuaacaacug cuauggcugc ugaaguaaca      120

aggaggggua gugcauauua uauguauuua gacagaaacg augcagggga agcaauaagu      180

uuuccuacaa cauuaggaau gaauaaaugu uauauccaaa uuauggacuu aggccauaug      240

ugugacgcua caaugaguua cgaaugucca auguuagaug aagguguaga accagacgau      300

guggauuguu gguguaacac aacaucaaca uggguaguau augguacaug ucaucauaag      360

aaaggagaag caagaagauc aaggagagcu guaacucuac cuucacacag cacaagaaaa      420

uuacaaacua gaucacagac cugguuagaa ucuagagaau auacaaaaca uuuaaucaga      480

guagaaaauu ggaucuucag aaaccccggg uuugcucucg cagcagcagc aauagcaugg      540

uuacuuggaa guucuacauc acaaaaaguu auuuaccuag uaaugauucu acuaauagca      600

ccagcuuaua gcauaagaug cauuggaguu aguaacagag acuuuguaga aggaauguca      660

ggugguacuu gggucgaugu gguauuagaa caugggggau gcguaacugu uauggcacaa      720

gacaaaccaa caguagauau cgagcuagua acaacaacgg uaagcaacau ggcagaagug      780

agaaguuauu guuaugaagc aagcauaagu gauauggcau ccgauucacg auguccaaca      840

caaggugaag cauaccuaga uaaacaauca gauacacaau auguauguaa aagaacauua      900

guugauaggg guuggggaaa uggaugugga cuauucggaa aagggucauu agugacgugu      960

gcaaaauuug ccugcaguaa gaaaaugacu gggaaaagca uucaaccaga aaaucuagaa     1020

uauagaauaa ugcuaagugu acauggaucu caacauagug gcaugauagu aaacgauaca     1080

ggacacgaaa cugacgaaaa cagagcuaaa guagaaauca cucccaauag cccaagagcu     1140

gaagcaacac uaggaggauu uggaucauua ggacuugacu gugaaccuag aacaggauug     1200

gacuuuagcg aucuauacua ucuaacaaug aauaauaaac auuggcuagu acauaaagaa     1260

ugguuucaug auauuccacu uccauggcac gccggcgcag acacagggac cccucacugg     1320

aacaauaaag aagcauuagu agaguucaaa gaugcucaug caaaaagaca aacaguaguu     1380

guauuagguu cgcaagaagg agccguacau acugcacuug caggagcuuu agaagcagaa     1440

auggauggag caaaaggacg auuaucuagu ggacaucuaa aauguagacu aaaaauggac     1500

aaauuaagau uaaaaggagu aagcuauuca uuauguacag cagcauuuac auucacaaaa     1560

auaccugcug aaacccuaca cggcacagua acaguagaag uucaauaugc aggaacugau     1620

ggaccuugca aaguacccgc acaaauggca guugauaugc aaaccuuaac cccuguuggc     1680

agacuaauca cagcaaaccc aguuauaaca gaaucaacag aaaauagcaa aaugaugcua     1740

gaacuggauc caccuuucgg ugauucauau auaguaauag gaguagggga aaagaaaauu     1800

acucaucacu ggcauagauc aggaucaaca aucgguaaag cauucgaagc uacaguacga     1860

ggggcaaaaa gaauggcagu acuaggagau acugcauggg auuuugguag uguaggugga     1920

gcacuaaauu ccuugggaaa aggaauacac caaauauuug gagcugcauu uaaaagucua     1980

uuuggaggaa ugagcugguu uagccagauu cuaauuggaa cauuacuuau guggcucgga     2040

uuaaacacaa agaauggaag uauuucauua auguguuuag cucucggugg cguauuaaua     2100

uuuuuaagua cagcaguuag cgcagacguu ggauguucag uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9093
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  9093
gggagaaagc uuaccaugac aagcguagga auuguuggac uauugcuaac aacugcaaug       60

gcugcagagg uaacucgaag aggcagcgca uacuacaugu auuuggauag aaaugacgca      120

ggggaagcaa uaagcuuucc aacgacauua ggaaugaaua aauguuauau acaaauaaug      180

gauuuaggac acauguguga ugcaacuaug aguuaugagu gcccaaugcu ugaugaagga      240

gucgaaccag augauguuga uuguuggugu aauaccacaa guacaugggu ugucuacgga      300

acaugucacc auaaaaaagg ugaagcuaga agaaguagaa gagcaguaac acuacccagu      360

cacucaacua gaaaacuaca aaccagaucc caaacauggu uagaaaguag ggaauacaca      420

aaacaucuaa uuagaguuga aaacuggaua uuuagaaauc cagguuucgc uuuagcagca      480

gcagcaauug cuugguuauu aggaaguuca acaucacaga aaguuauaua ccuaguaaug      540

auacuacuaa uugcacccgc auauaguaua cgauguauug gaguuagcaa ucgugauuuc      600

guugaaggaa ugucaggagg aacuuggguu gacauaguau uagaacaugg agggugugua      660

acgguuaugg cacaagauaa accaacgguu gauauagagc uaguaacaac aacaguuagc      720

aauauggcag aaguacgauc auauugcuau gaagcuucaa uaucagauau ggcaucagau      780

ucgagauguc caacacaagg ggaagcauau uuagauaaac aaagugauac acaauaugua      840

uguaaacgaa cacuaguaga uagagguugg gggaauggau gugguuuauu cggaaaagga      900

ucacuuguaa caugugcaaa guuugcaugu ucaaaaaaaa ugacaggcaa aagcauacaa      960

ccagaaaauc uagaauacag aaucauguua ucaguacaug gaucucaaca uagcggaaug     1020

auaguuaaug acacaggaca ugaaacagau gaaaauagag cgaaaguaga gauaacaccu     1080

aauaguccaa gagcagaagc aacauuggga ggauucggau cucuaggacu agauugugaa     1140

ccacgaacag gacuugacuu uucugauuua uacuaucuaa caaugaacaa uaaacacugg     1200

cuaguacaua aagaaugguu ucacgauaua ccacuaccau ggcacgccgg agcagauacc     1260

ggaacaccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc ccaugcuaaa     1320

agacaaacag uuguagugcu uggaagucaa gaaggagcag uacauacagc auuggcuggu     1380

gcauuagaag cagaaaugga uggugcaaaa ggaaggcuaa guucuggaca ucuaaaaugu     1440

aggcuaaaaa uggauaaauu aagauuaaaa ggaguaucau auucauuaug uaccgcugcc     1500

uucacuuuua caaaaauacc agcagaaacg cugcacggaa cuguaacagu ugaaguacaa     1560

uaugcuggaa cagauggacc augcaaaguu ccugcacaaa uggcuguaga caugcaaaca     1620

cugacuccug uugggagacu aauuacagca aaccccguaa uaacagaaag uaccgaaaac     1680

ucuaaaauga uguuagaauu agauccaccu uucggggaua guuauauagu aauaggagua     1740

ggggaaaaaa aaauaacuca ucacuggcau aggagcggca guacuauagg uaaagccuuu     1800

gaagcaacug ucagaggugc aaaaagaaug gcaguguuag gugacacagc augggauuuc     1860

ggaucaguag gaggugcauu aaauaguuua ggcaaaggca uucaucaaau auuuggggcc     1920

gcauucaaaa gucuguucgg aggaaugagc ugguucaguc agauauuaau aggaacacua     1980

uuaauguggc uaggucuaaa cacaaaaaac gggaguauua gucucaugug ucuugcauua     2040

ggaggaguau uaauauuuuu aaguacagca gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9094
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 3 opt

<400>  9094
gggagaaagc uuaccaugac cucaguaggu auaguagguu uacuauuaac aaccgcaaug       60

gcagcagaag ugacaagaag aggaucagca uacuauaugu auuuagauag aaaugaugca      120

ggagaggcaa uuucauuccc aacaaccuua ggaaugaaca aauguuauau acaaauaaug      180

gauuuaggac auacauguga cgcaacaaug aguuacgaau guccaaugcu agacgaaggu      240

guagaaccag augacguaga cugcuggugu aauacaacau caacaugggu uguuuacgga      300

accugucauc auaagaaagg ugaagcaaga agaaguagac gagccguaac ucuaccauca      360

cacucaacca gaaaacucca aacaagaagu cagacauggc uugaaagccg agaauacacu      420

aaacauuuaa uaagaguuga aaauuggauu uuuagaaacc caggauucgc auuagcagca      480

gcagcaauag cgugguuacu agguucauca acgucacaaa aaguuauuua uuuagucaug      540

auucuguuaa uagcaccagc uuacaguaua agauguaucg gaguaucaaa uagggacuuu      600

guugaaggaa uguccggagg uacuugggua gauguaguau uagaacaugg aggaugugua      660

acaguaaugg cacaagacaa accaaccgua gauauagagc uaguaacuac uacaguaucc      720

aauauggcag aaguacguag uuauuguuau gaagccagca uaagugauau ggcaucagau      780

ucaagaugcc caacacaagg ugaagcauau uuagauaaac aaagugacac acaauacgua      840

uguaaaagaa cauuaguaga cagaggaugg ggaaaugguu guggauuauu cgggaaggga      900

ucacuuguaa cuugugccaa auucgcaugc ucaaagaaaa ugacagggaa aucgauacaa      960

ccagaaaauu uagaauacag aauaaugcuc aguguacaug gaagccaaca uucaggaaug     1020

aucguuaaug auacugguca ugaaacagac gaaaacagag ccaaaguuga aauaacaccu     1080

aauucaccaa gagcugaagc aacauuagga ggauucggau cauuaggucu agacugugaa     1140

ccaagaacag gucuagacuu uagcgauuua uauuaccuaa cuaugaauaa uaaacauugg     1200

cuaguucaua aagaaugguu ucaugauaua ccacucccuu ggcaugcagg agcagacaca     1260

ggaacaccac auuggaauaa uaaagaagcc uuaguagaau uuaaagaugc acaugcuaaa     1320

agacagacag uuguuguacu cggaucucaa gaaggagcag uacauacagc auuagcagga     1380

gcauuagaag cagaaaugga uggugcaaaa gguagauuau cuagugguca ccuuaaaugu     1440

cgauuaaaaa uggauaaauu aagauuaaaa gggguuucau auagucuuug cacugcugcg     1500

uuuacauuua cuaaaauacc agcagaaacu cuacacggca caguaacugu agaaguacaa     1560

uaugcuggaa cugacggccc cuguaaagua ccagcgcaaa uggcugucga uaugcaaacc     1620

cuaacacccg uaggaaggcu gauaacagca aauccuguaa uaacagaaag uacggaaaau     1680

ucaaaaauga uguuagaacu agauccacca uucggcgauu cauacauagu aauuggagua     1740

ggagaaaaga aaauaaccca ccauuggcau aggagcggaa gcacaauagg aaaagcauuc     1800

gaagcaacag uaagaggugc aaaaagaaug gcaguacuag gagauacagc uugggacuuu     1860

ggauccguug guggagcauu aaauucguua gguaaaggaa uacaccaaau uuucggagca     1920

gcuuucaaau cccuauuugg aggaauguca ugguuuuccc aaaucuuaau aggaacacua     1980

uuaauguggc uaggccuaaa ugcuaaaaau ggaucuauau cauuaaugug cuuagcacua     2040

ggcggcguuc ugauauuuuu aagcacugca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9095
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 3 opt

<400>  9095
gggagaaagc uuaccaugac aaguauagga aucauuggau uacuacuaac aacugcaaug       60

gcagcagaaa uaacuagacg aggaucagca uauuacaugu auuuagauag auccgaugcu      120

ggcaaagcaa uuucauuugc aacaacacua ggaguaaaca aaugucaugu ucaaauaaug      180

gaucuaggac auauguguga ugccacaaug ucguaugagu guccaauguu agaugaaggu      240

guagaaccag acgauguaga uugcuggugu aauacgacau ccacaugggu aguauaugga      300

acuugucauc acaagaaagg agaggcaaga cgcuccagga gagcaguuac auuaccaagu      360

cauaguacaa gaaaauuaca aacaagaagc caaacuuggc uugaaucgag agaauauaca      420

aaacauuuaa uuaaaguaga aaauuggaua uuuagaaacc caggauucgc auuaguggca      480

gucgcaauug ccuggcuauu agggagcagu acaucacaaa aaguaauaua ucucguaaug      540

auacuauuaa uagcuccugc auacagcaua agaugcauag gaguuucaaa ccgagauuuu      600

guagaaggua ugagcggagg cacaugggua gacguuguac uagaacaugg aggauguguc      660

acugugaugg cacaagacaa accaacagua gauaucgaac ugguaacaac aacaguaagu      720

aauauggcag aaguaagauc cuauugcuau gaagcaucga uaucagacau ggcaagugau      780

ucuagaugcc caacucaagg agaagcauau uuagauaaac aaagcgacac acaauaugua      840

ugcaagagga cucuaguaga uagagguugg gguaauggau guggcuuauu uggaaaagga      900

agucucguaa caugcgcaaa guucacaugu ucaaagaaaa ugacuggaaa aucuauacaa      960

ccagaaaauu uagaguauag aauaaugcuc agcguacaug guucacaaca caguggaaug     1020

aucggauacg aaacugacga agaccgagca aaaguugaag uaaccccuaa uucaccaaga     1080

gcagaagcaa cauuaggagg guucggaagc cucggauuag acugcgaacc acgaacagga     1140

uuagauuuuu cagaucuaua cuauuuaaca augaacaaca aacauugguu aguucauaaa     1200

gaaugguuuc augauauacc acuaccaugg caugcaggcg cugauacagg cacuccacau     1260

uggaauaaca aagaagcauu aguagaauuu aaagacgcac acgcaaaacg acaaacagua     1320

guaguuuuag gaagccaaga aggagcggua cacacugcau uagccggugc auuagaagca     1380

gaaauggaug gugcaaaagg aagacuauuu ucuggucauu uaaaaugucg auuaaaaaug     1440

gauaaauuaa gacucaaagg aguaucuuac aguuuaugua cagcagcauu cacauucaca     1500

aaaguaccug cagaaacauu acauggaacg guuacaguag aagugcaaua ugcuggaaca     1560

gauggaccau guaaaauacc agugcaaaug gcuguagaua ugcagacuuu aacaccaguc     1620

ggaagauuaa uaaccgcaaa uccaguaaua acagaaagua ccgaaaauuc aaaaaugaug     1680

uuagaauuag auccaccauu uggcgacagu uauauuguua uaggaguagg cgauaagaaa     1740

auaacacauc acuggcauag aagcggaucu acaauaggaa aagccuuuga agcaacagua     1800

agaggagcca aaagaauggc aguacucggu gauacagcuu gggauuucgg aucuguugga     1860

ggaguuuuca auucauuagg caaaggaaua caucaaauau ucggagcugc cuuuaaauca     1920

uuauuuggug gaaugucaug guuuucucaa auucuaauag guacauuacu uguauggcua     1980

ggacuaaaca cuaaaaaugg uucaauauca cuaacauguc uagcuuuagg cggaguaaug     2040

aucuucuuaa gcacagcugu aucagcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9096
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 3 opt

<400>  9096
gggagaaagc uuaccaugac aucaguagga auagucggac uuuuguuaac gacagccaug       60

gcagcugaag uaacaagaag aggcucugca uacuauaugu aucuagaccg gaacgacgcc      120

ggagaagcaa uaucauuccc aacaacauua ggcaugaaua aaugcuacau acaaauuaug      180

gaucuuggac auaugugcga ugcaacaaug ucuuaugaau guccaauguu ggaugaagga      240

guugaaccug acgauguaga cugcuggugc aacacaacau caacaugggu uguauauggu      300

accugucauc auaaaaaagg cgaagccaga agaucaagaa gggcaguaac acugccuagu      360

cauaguacua gaaaauuaca aacuagauca caaacuuggu uagaaucaag ggaauacaca      420

aaacaccuaa uacgaguaga aaauuggaua uuucgaaauc caggguucgc acuagcagca      480

gcugcaauag cauggcuuuu aggaaguuca acaagccaaa aagucauaua ucuaguuaug      540

auucuacuua uagcaccugc auacucaaua cgaugcauag gagucucaaa uagggauuuu      600

guagaaggua ugucaggagg gaccugggua gacguaguac uagaacaugg uggaugugua      660

acagucaugg cacaagauaa accaacagua gacauagaau uaguaacaac gacugucucc      720

aauauggccg aaguuagauc auacugcuac gaagcuucua uaucagauau ggcaagcgau      780

ucaagauguc ccacccaagg ugaagcauau cuagacaaac aaucagauac acaauacguu      840

uguaaaagaa cauuaguaga cagagguugg ggaaauggau guggauuauu uggaaaaggu      900

aguuuaguaa caugcgcaaa auuugcuugc agcaaaaaaa ugacaggaaa auccauacaa      960

ccagaaaacc uugaguauag aauaaugcuc aguguucaug gaucacaaca uucuggaaug     1020

auaguaaaug acacaggaca cgaaacugac gagaaucgug cuaaaguaga aauaacccca     1080

aauaguccua gagcagaagc aacauuaggc ggauuuggaa guuuaggauu agauugugaa     1140

ccaagaacag gcuuggauuu uagugaucua uauuacuuaa caaugaauaa caaacauugg     1200

uugguccaua aagaaugguu ccaugauaua ccauuaccau ggcacgcugg agcagauacu     1260

ggaacaccgc auuggaauaa uaaagaagca cuuguugaau ucaaagacgc ucaugcuaaa     1320

cgacaaacag uaguuguacu cggaagccaa gaaggcgcag uccacacagc ucuagcagga     1380

gcguuagaag cugaaaugga uggcgcaaag ggaagauuaa gcucaggaca ccuaaaaugc     1440

agacuuaaaa uggacaaacu aagauuaaaa ggaguuaguu auagccuaug uacagcagca     1500

uucacauuua cuaaaauacc agcugaaaca uuacauggaa cuguuacagu agaaguacaa     1560

uaugcaggaa cagauggucc auguaaagua ccugcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccug ucggaagauu aauuacagca aauccuguaa uaacggaaag uaccgaaaau     1680

ucaaaaauga ugcuagaauu agacccaccu uuuggcgaua gcuacauagu aauuggagua     1740

ggagaaaaaa aaauaacaca ucauuggcac cgaaguggaa gcacaauagg aaaagcauuu     1800

gaagcaacag uuagaggagc aaaaagaaug gcaguauuag gggauaccgc cugggauuuc     1860

gguucuguag gaggugcacu aaauagucuu ggaaaaggca uacaccaaau uuuuggagca     1920

gcuuucaaau cacuguuugg uggaaugagu ugguuuucac aaauucuaau aggaacauua     1980

uuaauguggu uaggauuaaa uacaaaaaac ggaucaauaa gcuuaaugug ucuagccuua     2040

ggaggcguau uaauauuccu aucgacagcc guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9097
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 3 opt

<400>  9097
gggagaaagc uuaccaugag ucaaaaagua auauaucuag ucaugauauu auuaauagca       60

ccagcauauu caauaagaug uauaggaguc ucaaacaggg auuucguaga aggaaugagu      120

ggagguacgu ggguagauau aguuuuagaa cauggaggau guguaaccgu uauggcacaa      180

gacaaaccaa caguagacau agaguuaguc acaacaacag uaagcaauau ggcagaagua      240

agaucauacu guuaugaagc aaguauaagu gauauggcaa gugacucaag auguccaacu      300

cagggagaag cauaccuaga caaacaauca gacacacaau acguauguaa acgaacacua      360

guggauagag gauggggaaa ugguugugga cuauuuggaa aaggaagccu gguuacaugc      420

gcuaaauuug ccugcucaaa aaaaaugaca ggaaaaucaa uacaaccaga aaaucuagaa      480

uaucggauua uguuaagcgu acauggaagc caacacucag gaaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa ucgagcaaaa guggaaauaa ccccaaacag cccaagagca      600

gaagcuacac uaggaggauu uggauccuua ggacuagauu gugaacccag aacaggacua      660

gacuuuucug auuuauacua ucuuacaaug aauaauaaac auugguuagu acacaaagaa      720

ugguuccaug auauaccauu accauggcau gcaggagcag auacgggcac accacacugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaaagaca aacaguagua      840

guauuaggau cacaagaagg cgcaguucau acagcccuag caggagcacu agaagcagaa      900

auggauggag caaaaggaag gcuuagcagc ggucauuuaa aaugcagauu aaaaauggau      960

aaauuaagac uaaaaggagu aaguuauagu cuauguacag ccgcauuuac auuuacaaaa     1020

auaccagccg aaacuuuaca uggaacagua acaguagagg uacaauaugc aggaacggac     1080

ggaccaugua aaguaccagc acaaauggcu guagacaugc aaacacuaac cccaguaggc     1140

agauuaauaa cagcuaaucc aguuauaaca gaaaguacag aaaauucuaa aaugaugcua     1200

gaauuagacc caccuuuugg agacucauau auaguaauag guguaggaga aaaaaaaaua     1260

acacaucauu ggcauagauc aggaaguaca auaggaaaag cauucgaagc aaccguaaga     1320

ggagccaaaa gaauggcggu acuaggugau acagcauggg auuuuggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9098
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 3 opt

<400>  9098
gggagaaagc uuaccaugag ucaaaaaguu auauauuuag uuaugauuuu acuuauagcu       60

ccggcauaca guauaagaug uauaggagua ucaaauagag auuucguaga gggaaugagu      120

ggaggaacau ggguagaugu aguauuagaa cauggagguu guguaacagu aauggcacaa      180

gacaaaccaa caguagauau agaauuaguu acaaccacag uaucaaauau ggcagaagua      240

agaagcuauu gcuaugaagc aaguauaagu gacauggcaa gugauagcag auguccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacacaau acguuuguaa aagaacauua      360

guagauagag guuggggaaa cggauguggu uuauuuggaa aagggucuuu aguaacuugu      420

gcgaaauuug cuugcaguaa aaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa      480

uauaggauaa uguuaucagu acauggaucu caacauagcg guaugauagu aaaugacaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccgaauuc gcccagagca      600

gaagcaacac ugggaggauu uggaagccua ggacuagauu gugaaccaag aacgggacua      660

gauuuuucag aucuguauua ucuuacaaug aauaauaaac auuggcuagu gcauaaagag      720

ugguuucaug auauaccacu accauggcau gcaggagcag acacagguac accacauugg      780

aauaauaaag aagcacuagu agaauucaaa gacgcacaug caaaaagaca aacaguuguu      840

guacuaggaa gccaagaagg agcaguacau acagcauuag caggggcauu agaagcagaa      900

auggacggag caaaagguag acuaucgagc ggacaccuaa aauguagauu aaaaauggau      960

aaacuacgau uaaaaggagu aaguuacagu uuaugcacag cugcauuuac auuuacaaaa     1020

auaccggcag aaacauuaca uggaacgguu acaguagaag uacaauacgc uggaacagau     1080

ggacccugua aagugccugc gcaaauggca guggacaugc aaacauugac accaguagga     1140

agacuaauaa cagcaaaucc aguaauaaca gaaaguacgg aaaauaguaa aaugauguua     1200

gaacuagauc cuccauuugg agacucauau auaguaauag gaguaggaga aaaaaaaauc     1260

acacaccacu ggcacagauc aggaucgaca auaggaaaag ccuuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcugu auuaggggac acagcauggg acuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9099
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 3 opt

<400>  9099
gggagaaagc uuaccauguc acaaaaagua auuuaucuag uuaugauauu auuaauagcu       60

ccagcauaca guauaagaug uauaggagua ucaaacagag acuuuguaga aggaauguca      120

ggaggaacau ggguugacgu aguauuagaa cacgguggau gcguaacagu aauggcacag      180

gacaaaccaa caguugauau agaauuaguu acaacaacag uuucaaauau ggcugaagua      240

agaucauauu guuaugaagc aaguauaagu gacauggcca gcgacaguag augcccuaca      300

caaggagaag cauaccuaga uaaacaaagc gauacacaau acgucugcaa aagaacccua      360

guagacagag guuggggcaa cgguugugga cuauuuggua aagguagcuu gguaacaugu      420

gcaaaauuua cauguucaaa gaaaaugaca ggaaaaucaa uacaacccga aaaucuugaa      480

uauagaauaa ugcugagugu acauggaagu caacauucag gaaugaucgg auacgaaaca      540

gacgaagaua gagcaaaagu agaaguaaca ccaaauucac cgagagcaga agcaacacua      600

ggaggauuug gcucacuagg auuagacugu gaaccaagaa caggacuaga uuucagugau      660

cuauacuacc uaacaaugaa caacaaacac ugguuaguac auaaagaaug guuucaugac      720

auaccauuac cuuggcacgc aggagcagau acaggaacac cacauuggaa uaauaaagaa      780

gcauuagugg aauuuaaaga ugcucaugca aaaagacaaa caguaguagu acuaggaagu      840

caagaaggcg ccguacacac agcucuagca ggagcauuag aagcagaaau ggauggagca      900

aaaggaagau uauuuagugg acacuuaaaa uguagauuaa aaauggauaa acuaagauua      960

aaagguguau cauauaguuu auguacagca gcuuucacau uuacaaaagu accagcagaa     1020

acauuacaug gaacagucac aguagaagua caauacgcag gaacagaugg accauguaaa     1080

auaccaguac aaauggcagu agacaugcaa acacuaacuc caguaggaag guuaauaaca     1140

gcaaauccag uaauaacaga aaguacagaa aauaguaaaa ugauguuaga auuagauccc     1200

ccauuuggag acaguuauau agugauaggc guaggagaca agaaaauaac acaucauugg     1260

cauagaucag guucaacuau aggaaaagca uuugaagcua caguaagagg ugcgaaaaga     1320

auggcaguac uaggagauac agcaugggac uuuggaaguu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  9100
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 3 opt

<400>  9100
gggagaaagc uuaccaugag ucaaaaaguu auauauuugg uaaugauauu acuaauugcu       60

ccagcauauu caauacgaug cauaggagua ucaaauagag auuucgucga aggaaugagu      120

ggaggaacau ggguagaugu aguauuagaa cauggaggau gcguaacagu aauggcacaa      180

gauaaaccaa caguagauau agaacuagua acaacaaccg uaucaaacau ggcagaagua      240

agaucauauu guuaugaagc aucaauaagc gacauggcgu cugauaguag augcccaaca      300

caaggugagg cauauuuaga uaaacaaagc gauacacaau auguuugcaa aagaacauua      360

gucgauagag gauggggaaa uggaugugga uuguucggaa aaggaucacu agucacaugu      420

gcaaaguuug cauguaguaa aaaaaugaca ggaaaaagua uccaaccaga aaacuuagaa      480

uauagaauaa ugcucagugu acaugguagu caacauagug gcaugauagu gaaugauaca      540

ggucaugaaa cagaugaaaa ucgagcgaaa guagaaauua cacccaauuc uccuagagca      600

gaagcaacuu uaggaggauu uggaaguuua ggauuagauu gcgaaccaag aacaggauua      660

gacuucagcg aucuauacua uuuaacaaug aacaauaaac auuggcucgu acauaaagaa      720

ugguuucaug acauaccucu accuuggcac gcaggagcag auacaggaac uccacauugg      780

aauaauaaag aagccuuagu agaauuuaaa gaugcacaug caaaaagaca aacaguaguc      840

guacuaggaa gccaagaagg agcaguacau acugcauugg caggagcauu agaagcagaa      900

auggacggug caaaaggaag guuaucaagu ggacaucuaa aaugcagacu uaaaauggau      960

aaauuaagau uaaaaggagu aaguuauuca uuaugcacag cagcauuuac auuuacaaaa     1020

aucccagcag aaacacuaca uggaacagua acaguagaag uacaauacgc aggaacugau     1080

ggaccaugua aaguaccagc acaaauggcc guugauaugc aaacauuaac accaguagga     1140

agauuaauaa cagcaaaucc aguaauaaca gaaaguaccg aaaacaguaa aaugauguua     1200

gaacuagauc cuccauuugg agacaguuac auaguuauag gaguaggaga aaaaaaaaua     1260

acacaccauu ggcacagaag uggaagcaca auagguaaag cauuugaagc aacaguaagg     1320

ggagcaaaaa gaauggcagu acuaggagau acagcauggg auuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9101
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       3 opt

<400>  9101
gggagaaagc uuaccaugac aucaguagga auuguuggau uauuacuaac aacagcaaug       60

gcagcagaag uaacaagacg aggaagcgca uauuauaugu aucuggauag aaaugaugcu      120

ggugaagcaa uaaguuuucc aacaaccuua gguaugaaua aauguuacau ucaaaucaug      180

gauuuaggac acauguguga ugcaacaaug ucauaugaau guccuaugcu agaugaaggc      240

guugaaccag acgaugugga uuguuggugc aacacaacaa guacaugggu gguauauggu      300

acgugucauc acaagaaagg agaagcaaga agaucaagga gagcaguuac acuuccaagc      360

cauaguacaa gaaaauuaca aacaagaagu caaacauggu uagaaucaag agaguacaca      420

aaacaccuaa uuagaguaga aaauuggaua uucagaaauc caggguucgc acucgcagca      480

gcagcuauag caugguuacu aggaaguagu acaagucaaa aaguaauaua ucuaguaaug      540

auacuauuga ucgcgccagc auauucuaua agauguauag gaguaagcaa uagagauuuu      600

guagaaggua ugucuggagg uacaugggua gauauaguau uagaacacgg cggaugcguc      660

acaguaaugg cccaagauaa accaacagua gauauagaac ucguaacaac aacaguuagu      720

aauauggcag aaguaagaag cuacuguuac gaagccagua uaucagacau ggcaagugau      780

ucaagauguc caacacaggg agaagcauau uuagauaaac aaucugacac acaauauguu      840

ugcaaaagaa ccuuaguaga cagaggaugg ggaaacggau gcggacuaag cggaaaaggu      900

agcuuaguaa caugugcaaa auucgcaugc ucaaagaaaa ugacuggaaa aagcauacaa      960

ccagaaaacu uagaauauag aauuauguua aguguacaug gaucacaaca caguggaaug     1020

auaguaaacg acacuggaca ugaaaccgau gaaaaucgag caaaaguaga aauaacaccu     1080

aauagcccga gagcggaagc cacacuagga ggauucggaa guuuaggacu agacugugaa     1140

ccaagaacag gauuagacuu uagugaucua uauuauuuaa ccaugaacaa uaaacauugg     1200

uuaguucaua aagaaugguu ccaugacaua ccauuaccau ggcacgcagg agcagauacc     1260

gguacgccgc auuggaauaa caaagaagcu cuaguagaau uuaaagaugc acaugcaaaa     1320

cgacaaacug uagugguacu aggaucacaa gaaggagcag uucauacagc uuuagcgggg     1380

gcgcucgaag cagagaugga cggugcaaaa gguagauuaa guucaggaca ccuaaaaugu     1440

agacuaaaaa uggauaaacu aagauuaaaa ggaguaucgu auucuuuaug uacagcugcg     1500

uuuaccuuua caaaaauucc agcagaaaca uuacauggaa ccgucacagu ugaagugcaa     1560

uacgcaggaa cugauggucc augcaaaguc ccugcacaga uggcaguaga uaugcaaacg     1620

cuaacaccgg uaggacgauu gaucacugcg aauccuguga uaacugaaag caccgaaaau     1680

ucaaaaauga ugcuggaauu agauccucca uuuggagaca guuacauagu aauaggagua     1740

ggagaaaaga aaauaacaca ucauuggcac agaucaggaa guacaauagg caaagcauuu     1800

gaagcuacag ugaggggagc aaaacgaaug gcaguauuag gugauacagc augggacuuu     1860

ggcaguguag gaggggcacu aaauucuuug ggaaaaggua uacaucaaau cuuuggagca     1920

gcauuuaaaa guuuauuugg aggaaugagc ugguuuucac aaauuuugau aggcacuuua     1980

uuaauguggu uaggacuaaa uacuaaaaau ggaucaauau cauuaaugug uuuagcuuua     2040

ggaggcguau uaauauuuuu auccacagca gugagcgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9102
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 3
       opt

<400>  9102
gggagaaagc uuaccaugac auccguagga auuguaggau uauuacuaac aaccgcaaug       60

gcagcagaag uaacgcgacg aggaagugca uauuacaugu acuuagauag aaaugacgcu      120

ggugaagcaa uaaguuuucc uacaacucuu gggaugaaca aauguuacau ccaaauaaug      180

gaucuuggcc auacauguga ugcaacaaug ucauaugaau guccaauguu agaugaagga      240

gucgaacccg acgaugucga uuguuggugc aauaccacau caacaugggu uguauaugga      300

acaugccauc auaagaaagg agaagcgcgg agaaguagac gagcaguaac auuaccauca      360

cauucgacac gaaaauuaca aacaagaagu caaacauggu uagaaagcag agaauacaca      420

aaacauuuaa uaagaguaga aaacuggaua uuuagaaauc caggguuugc auuagcagca      480

gcagcaauag cauggcuucu aggauccagu accucacaaa aaguaauaua cuuaguaaug      540

auauuauuga uugcaccagc uuauucuaua agauguauug gaguuagcaa uagagauuuu      600

guagaaggga ugaguggagg aacaugggua gauguaguuu uagaacacgg agguugugua      660

acaguaaugg cacaagauaa accaacagua gauauagagc uaguaacaac uacugugagc      720

aauauggcag aaguucgauc auacuguuac gaagcaucaa uaagugacau ggcaucggau      780

agcagauguc caacacaagg ugaagcauau cuagauaaac aaucagauac ucaauaugua      840

uguaaacgaa cuuuaguaga uagaggaugg ggaaauggau gcgguuuaag cggaaaaggc      900

ucauuaguua caugugcuaa auucgcuugu aguaagaaaa ugacaggaaa aucaauacaa      960

cccgaaaauc uagaauaucg aauaauguua uccguacaug gaagccaaca uucagguaug     1020

auaguaaaug acacaggaca ugaaacagac gaaaauagag cuaaaguaga aauaacuccu     1080

aauucaccaa gagcagaagc uacacuagga gguuucggua gccuaggguu agauugugaa     1140

ccaagaacag gauuagacuu uucagacuua uauuaccuaa caaugaauaa uaaacauugg     1200

uuaguucaca aagaaugguu ucacgauaua ccauugccuu ggcaugcagg agcagauacu     1260

ggaacaccac acuggaauaa uaaagaagca uuaguagaau uuaaagaugc acacgcaaaa     1320

agacagacag uugucguauu agggucacaa gaaggagcug uacauacagc auuagccgga     1380

gccuuagaag cagaaaugga cggagcaaaa ggaagacuaa guagcggaca ucuuaaaugu     1440

agacuaaaaa uggacaaacu acgacugaaa ggaguaucau auucauuaug uacagcagcu     1500

uuuacauuca caaaaauacc agcugaaacc cuacauggaa caguaacagu cgaaguucaa     1560

uacgcaggaa cggacggacc auguaaagua ccagcucaaa uggcaguaga caugcagacu     1620

uuaacaccag uagggaggcu aauuacagca aacccuguua uaacagaauc aacggaaaac     1680

ucaaaaauga uguuggaauu agauccaccu uuuggagacu cauacauagu aauaggagua     1740

ggagaaaaga aaauaacaca ucacuggcau cgaaguggau caacuauugg uaaagcauuu     1800

gaagcaaccg uaagaggagc uaaaagaaug gcuguuuuag gugauacagc gugggauuuu     1860

ggcucagugg gaggugcauu aaacucuuua ggcaaaggga uacaccaaau auuuggugcc     1920

gcauuuaaau cauuauucgg aggcauguca ugguucagcc aaauauuaau aggaacauua     1980

uuaauguggu uaggucuaaa cgcaaagaac ggaaguauuu cucuaaugug uuuagcauua     2040

ggaggaguuc uaauauuucu aucaaccgca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9103
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 3 
       opt

<400>  9103
gggagaaagc uuaccaugac aucaauugga auaaucggcu uauugcuaac uacagcaaug       60

gcagcugaaa uuacaagaag aggaucagcu uauuauaugu aucuagacag aucagaugcu      120

gguaaagcaa uuucauucgc aacaacacua ggcguaaaua aaugucacgu acaaauaaug      180

gauuuaggcc auaugugcga ugcaacaaug ucguaugaau guccaauguu agacgaagga      240

guggaacccg augauguaga cuguuggugc aacacaacuu caacaugggu aguauacgga      300

acaugccauc acaaaaaagg agaagcaaga agaucaagac gagcaguuac ucuaccaagc      360

cauaguacaa ggaaacuaca aaccagauca caaacauggc uagaaucacg ugaauacaca      420

aagcauuuaa uaaaaguaga aaacuggaua uuuagaaauc caggguucgc auuaguggca      480

guagcaauag cauggcuauu aggaucauca acaucacaaa aaguaauaua uuuaguaaug      540

auauuauuaa uagcgccagc auauaguaua cgauguauag gaguaucaaa cagagauuuu      600

guggaaggaa ugucaggagg uacaugggug gacguaguuc uagaacacgg aggcugugua      660

acaguaaugg cacaagacaa accuaccgua gauauagaac uagugaccac aacaguauca      720

aacauggcgg aaguaagguc uuauugcuau gaagccagua uaucagauau ggcaucugau      780

agcagaugcc caacacaagg agaagcauau cuugauaaac aaucugauac acaauaugua      840

uguaaaagaa ccuuaguaga ucgaggaugg ggaaaugguu guggacuauc agguaaaggu      900

ucauuaguca caugugcuaa auucacuugu aguaaaaaaa ugacaggaaa aucuauacaa      960

ccagaaaauc uugaauauag gauaauguua aguguccacg gaagucaaca uucagggaug     1020

auagguuaug aaacagauga agacagggca aaaguagaag uaacaccaaa cagcccaaga     1080

gcggaagcaa cauuaggagg auucggaagc cucggacuag acugugaacc acggacggga     1140

uuagacuuca gcgaucuuua uuauuuaaca augaauaaua aacacuggcu aguucauaaa     1200

gaaugguucc augauauccc ucuaccaugg caugcaggag cagauacagg aacaccccac     1260

uggaauaaua aagaagccuu aguagaauuu aaagaugccc acgccaaaag acaaacggua     1320

guuguauuag gaucacaaga aggagcagua cauacggcgu uagcuggcgc ccuagaagca     1380

gaaauggaug gugcgaaagg aagacuauuu ucaggccauc ucaaaugcag auugaaaaug     1440

gauaaacuua gacuaaaagg aguaucauau ucauuaugua cagcagcauu uacauuuacc     1500

aaaguaccag cagaaacauu acauggaaca guuacaguag aaguucaaua ugcagguaca     1560

gauggaccuu guaagauacc aguacaaaug gcgguagaua ugcaaacacu cacuccaguu     1620

gguagauuaa uuacugcaaa cccaguaaua acagaaucga cggaaaauuc uaaaaugaug     1680

uuagaauuag acccaccauu uggagauagu uauauaguga uagguguggg agauaaaaaa     1740

auaacacauc auuggcaucg aucaggguca acuauaggua aagcauucga agcaacagug     1800

agaggggcaa aaaggauggc aguacuagga gauacugcau gggauuuugg cagugucggu     1860

gggguuuuua auagccuagg uaaaggaaua caccaaauau uuggcgcagc guucaaauca     1920

uuauuuggcg gaaugucaug guuuucucaa auacuuauag gaaccuuacu aguaugguug     1980

gguuuaaaca ccaaaaaugg aaguauuuca cuaacauguu uagcccuggg aggaguaaug     2040

auauuucuaa gcacagcagu aagugcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9104
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F399S) mRNA Design 3 
       opt

<400>  9104
gggagaaagc uuaccaugac aucaguagga auaguaggac uacuauuaac aacagcaaug       60

gcagcugagg uaacaagaag aggaagcgcu uauuauaugu auuuagauag aaaugaugca      120

ggagaagcua ucagcuuccc aacaacacua ggaaugaaca aauguuauau acaaauaaug      180

gauuuaggcc acaugugcga ugcaacaaug agcuaugaau guccaauguu agaugaagga      240

guagaaccug augauguuga uuguuggugc aacaccacaa guacaugggu aguuuaugga      300

acaugccauc acaagaaagg ugaagcacga agaucaagac gagcaguaac acugccaucu      360

cauucaacac gaaaauuaca gacaagaagu cagacauggc uugaaaguag agaauauacg      420

aaacaccuaa uacgcguaga aaauuggaua uuuagaaacc cuggauuugc cuuggcggca      480

gcagcaauag cuugguuauu agguucuagu acaucacaaa aaguaauaua ucuaguaaug      540

auacuacuaa uagcaccagc auacucaaua agaugcauag gaguaagcaa cagagauuuc      600

guagaaggaa ugaguggagg aaccugggua gauguagucc uagagcacgg aggaugugua      660

acaguaaugg cacaagacaa accaacugua gauauagaac uuguaacaac aacgguauca      720

aacauggcag aaguacgaag uuacugcuac gaagcaucaa uaucugacau ggcuucagau      780

ucaagaugcc caacacaagg agaagccuau cuagauaaac aaucagacac acaauacguu      840

ugcaaacgaa cauuaguaga ccgagguugg ggaaacggau gugggcuauc agggaaaggu      900

ucacuuguaa caugugcaaa auuugcaugc ucaaagaaaa ugaccgguaa aaguauucaa      960

ccagaaaauc uagaauacag aauuaugcua ucaguacacg gaucacaaca uagugguaug     1020

auaguaaaug acacuggaca ugagacugau gaaaauagag caaaaguaga aauaacacca     1080

aauaguccaa gagcagaagc aacauuagga ggauuuggua guuuaggacu cgacugugaa     1140

ccacggacug ggcuggauuu uagugacuua uauuacuuaa ccaugaauaa uaaacacugg     1200

uuaguacaca aagaaugguu ccaugauaua ccuuugccau ggcaugcugg ugcagauaca     1260

ggaacucccc auuggaauaa uaaagaagca cuuguagaau uuaaagaugc acacgcaaaa     1320

cgacaaacag uaguuguacu agguucccaa gaaggagcag uacauacagc acuugcagga     1380

gcucuagaag cugaaaugga uggagcuaaa ggacgauuaa guaguggaca ccuaaaaugu     1440

agacuuaaaa uggacaaauu aagguuaaaa ggaguaaguu auucauuaug uacagcagca     1500

uucaccuuca cuaaaauacc ugcggaaaca cuccacggua caguaacugu agaaguacaa     1560

uaugcaggaa cagauggacc auguaaagua ccagcacaaa uggcugugga uaugcaaaca     1620

cuaacucccg uaggaagacu aauaacugca aacccaguaa ucaccgagag uacagaaaac     1680

ucuaaaauga uguuagaauu agauccacca uuuggagauu cauacauugu aauuggagua     1740

ggugaaaaga aaauaacuca ccauuggcau agaucuggaa guacaauagg aaaagccuuc     1800

gaagcaacag uaagaggugc uaaacggaug gcaguauuag gagauacggc augggauuuu     1860

gguucuguag guggagcacu aaacucccua gggaaaggga uucaccaaau uuuuggagca     1920

gcauuuaaau cauuauuugg aggcaugagc ugguuuucac agauacuaau agguacauua     1980

cuuauguggu uggguuuaaa cacuaaaaac gguucaauuu cauuaaugug cuuagcacua     2040

gguggugugu uaauauuucu aaguacagcg guuagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9105
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       3 opt

<400>  9105
gggagaaagc uuaccaugac cagcguagga auuguagggc ucuuauuaac aacagcuaug       60

gcagcagaag ugacaagaag ggguagugcc uauuacaugu acuuagauag aaaugacgca      120

ggugaagcaa uuucauuucc aacaacauua ggaaugaaua aauguuacau acaaauaaug      180

gaccuaggac auauguguga cgcaacaaug aguuaugaau gucccauguu agacgagggg      240

guugaaccag augauguaga uugcuggugu aauacaacga guacgugggu aguauaugga      300

acgugucauc auaaaaaagg agaagcucgu agaaguagga gagcaguaac uuuaccaagu      360

cacucaacaa gaaaauuaca aacaagaagc caaacauggu uagaaucaag agaauauaca      420

aaacaucuca uaagaguaga aaauuggaua uuuagaaauc caggauuugc auuagcagcu      480

gcugcaauug ccugguuacu cggaaguucc accucacaaa aaguaauaua ccucguaaug      540

aucuuguuaa uagcaccagc uuauucgaua agauguaucg gaguuaguaa uagagauuuu      600

guugaaggca ugagcggagg gacaugggua gauauaguac uugaacaugg cggauguguu      660

acaguaaugg cacaagauaa accaacggua gauauugaau uaguaacaac aacagucagu      720

aacauggcug aaguaagauc auauugcuau gaagcuagua uaucugauau ggcuagugau      780

aguagauguc caacucaagg agaagccuau uuagauaaac aaagugacac ucaauauguu      840

uguaaaagaa cauuaguaga cagaggaugg ggaaaugguu guggauuauu cggaaaagga      900

agcuugguaa caugugcaaa auucgcaugu aguaaaaaaa ugacaggaaa aucuauucaa      960

ccagaaaacu uagaauauag gauaauguua ucagugcacg gcucacaaca uaguggaaug     1020

auaguacaag acacagggca ugaaacagau gaaaauagag caaaaguaga aauaacucca     1080

aacaguccua gagcugaagc aacucuaggu ggauuuggaa guuuaggcuu agauugugaa     1140

ccaaggacag gauuagauuu uagugauuua uauuaccuaa caaugaauaa uaaacauugg     1200

cuuguacaua aagaaugguu ccaugauauu ccacuaccau ggcaugcagg agcagacacu     1260

gguacuccac auuggaauaa caaagaagca uuaguugaau uuaaagaugc ccaugcuaaa     1320

agacaaacag uaguaguauu aggaagucaa gaaggagcug uucacacugc acuugcagga     1380

gcacuagaag cagaaaugga uggagcaaaa ggacgguuaa gcaguggaca uuuaaaaugc     1440

agacuaaaaa uggauaaacu ucgauuaaaa gggguaucau auaguuuaug uacugcagcu     1500

uuuacguuua caaaaauacc agcggaaaca cuacauggaa caguuacagu ugaaguacag     1560

uaugcaggca cugauggacc uuguaaggua ccagcgcaaa uggcaguaga caugcaaaca     1620

cuaacuccag ugggacgacu aauaacagca aauccaguaa uaacagaaag cacagaaaau     1680

aguaaaauga ugcuagaacu agacccacca uuuggagacu cauacauagu cauaggcgua     1740

ggagaaaaaa aaauaaccca ucauuggcac agaaguggau caacuauagg aaaagcauuu     1800

gaagcaacag uucguggagc aaagagaaug gcaguauugg gagacaccgc augggauuuc     1860

gguaguguug gaggugcacu uaauucauug ggaaaaggaa uacaucaaau uuuuggagca     1920

gcuuucaaaa guuuauucgg cgggauguca ugguucaguc aaauauuaau agguacuuua     1980

cuaauguggu uaggauuaaa uacaaaaaau gguagcauuu cacuuaugug ucuagcauua     2040

ggagguguuu ugauauuucu aucaacagcu gugucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9106
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 3
       opt

<400>  9106
gggagaaagc uuaccaugac aucagucgga auagucggac uucuauuaac uacugcaaug       60

gcagcagaag ucacaagaag aggaucagcu uauuacaugu acuuagauag gaaugaugcu      120

ggagaagcca uuagcuuucc caccacuuua ggcaugaaua aauguuacau acaaauaaug      180

gauuuaggac auacauguga ugcaacaaug ucauacgaau gucccauguu agaugaaggu      240

guagaaccag augauguuga uuguuggugc aauacaacau caacaugggu aguauacgga      300

acaugccauc auaagaaagg agaagcaagg cgaucaagac gagcaguuac auugccuagu      360

cauaguacca gaaaauuaca aacaagaucu caaacauggu uagaaaguag agaauacaca      420

aaacaucuaa uaagaguuga aaacuggaua uucagaaauc caggauuugc acuagcagca      480

gcagccauag cauggcuauu aggaucaagc acaagucaaa aaguuauaua cuuagugaug      540

auauuauuaa uagcaccagc uuacucuaua agauguaucg gagucuccaa cagagauuuu      600

guagaaggaa ugucaggagg aacauggguu gauguaguau uagaacaugg uggaugugua      660

acaguaaugg cacaagauaa accaaccgua gauauagaau uagucacaac aacuguauca      720

aauauggccg aaguaagaag uuacuguuau gaagcaucaa uuucugacau ggcuucagau      780

agccgcugcc ccacacaagg cgaggcauau uuagacaaac aaagcgauac acaauaugua      840

uguaaaagaa cucuaguuga uagaggaugg gguaauggau guggacuauu cgguaaaggu      900

ucauuaguaa caugugcaaa auuugcuugu agcaagaaaa ugacagggaa aucaauccaa      960

ccagaaaauc uagaauauag aauaauguua ucagugcacg gaagucagca cucagguaug     1020

auaguucaag auacaggaca ugaaacagac gaaaauagag caaaagugga aaucacgcca     1080

aauucaccaa gagcugaagc uacccuaggu ggcuuuggau cauuaggcuu agauugcgaa     1140

ccaaggacag gauuagauuu uucagaucuc uacuauuuaa caaugaauaa uaaacacugg     1200

cuaguccaca aagaaugguu ccaugauauc ccauugccuu ggcaugcugg agcagauaca     1260

gguacaccac auuggaauaa caaagaagca cucguagagu ucaaagaugc acaugcaaaa     1320

agacaaacag uaguaguacu aggaucccaa gaaggugcag uucacacugc acuggcagga     1380

gccuuagaag cagaaaugga uggagcaaaa ggaagauuau caaguggaca ccuaaaaugu     1440

agauuaaaaa uggauaaacu aagauuaaaa ggaguauccu auucccucug caccgcagcc     1500

uucacuuuua cuaaaauacc agcagaaaca uugcauggaa caguuaccgu agaaguacaa     1560

uacgcaggaa cugauggacc cuguaaagua cccgcacaaa uggcaguaga uaugcaaaca     1620

cucacaccag uaggaagauu aauaacagca aauccaguaa uaaccgaauc aacagaaaac     1680

agcaaaauga ugcuugaauu agauccaccu uuuggagauu cauauauagu aauuggugua     1740

ggcgaaaaga aaaucacaca ucauuggcau agaaguggau caaccauagg aaaagcauuu     1800

gaagcaacag uaagaggggc aaaacgaaug gcgguccuag gagacacggc augggauuuu     1860

ggaucaguag gaggagcauu aaauucacua ggaaaaggca uucaucaaau auucggagcu     1920

gcauucaaaa guuuauucgg aggaauguca ugguuuaguc aaauacuuau aggcacauua     1980

uuaauguggc uaggauuaaa cgccaaaaau gggucaauau cacucaugug uuuagcuuua     2040

gguggaguau uaauauuuuu auccacagca guaucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9107
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 3 
       opt

<400>  9107
gggagaaagc uuaccaugac aagcguggga auaguuggac uacuauuaac aacagcaaug       60

gcagcagaag ugacucgucg gggaagugca uauuauaugu auuuagauag aaaugacgca      120

ggagaagcaa uuaguuuucc aacaacauua ggaaugaaua aauguuacau acaaauuaug      180

gaucuaggac acauguguga ugcaacaaug ucauaugaau guccgaugcu agacgaagga      240

guagagccag augauguuga cugcuggugc aauacaacaa gcacaugggu agucuacgga      300

acuugccauc auaagaaagg agaggcacga agaagccgga gagcuguaac uuuaccuagc      360

cauucuacuc gaaaauuaca aacaagauca caaacuuggc uagaaagucg agaauauaca      420

aaacauuuaa uaagaguaga aaacuggaua uuccguaauc cuggguuugc ucuugcagca      480

gcagcaauag cguggcuacu aggaaguagc acaagucaaa aaguaauaua cuuagugaug      540

auauuauuaa uagcuccagc auauucaauu agauguaucg gugugaguaa uagagauuuc      600

guagaaggaa ugaguggagg gacuugggua gauguaguuu uagaacacgg aggauguguc      660

acagugaugg cucaagacaa accaacugua gacauagaac uuguaacuac cacaguauca      720

aacauggcag aaguuagaag uuauuguuau gaagcaucua uaucagauau ggcaucagau      780

ucacgauguc caacacaagg cgaagcguau cuagauaaac aaucggauac ucaauaugua      840

uguaaaagaa cuuuaguaga uagaggaugg ggaaauggau guggccuauu cggaaaagga      900

ucauuaguga caugcgcuaa auuugccugu agcaagaaaa ugaccgguaa aaguauucaa      960

ccagaaaauu uagaauauag aauaauguua ucaguucacg gaucacaaca uaguggaaug     1020

auaguucaag auacaggcca cgaaacugac gaaaauagag caaaaguaga aauaacacca     1080

aauucuccaa gagcagaagc aacacuaggc ggauuuggau cauuaggauu ggauugugaa     1140

ccucgaacag gauuagacuu uagugauuug uauuauuuaa caaugaacaa caaacauugg     1200

uuaguacaca aagaaugguu ucacgauaua ccacuaccau ggcaugcagg agcagauacg     1260

ggaacacccc auuggaauaa uaaagaagcg cuuguagaau uuaaagaugc ucaugcaaaa     1320

aggcaaacag uaguaguauu aggaagucaa gaaggagcag uacauacagc ucuagcugga     1380

gcccuggaag cugaaaugga uggggcuaaa ggaagacuua gcucaggaca ucuaaagugc     1440

cgauuaaaga uggacaaauu aagauuaaaa ggcguaaguu auucuuuaug uacagcagca     1500

uuuacauuua caaaaauacc agcagaaacg cugcauggua cuguaacagu agaaguacaa     1560

uaugcaggaa cugacggacc auguaaagua ccagcacaaa uggcgguuga uaugcaaaca     1620

uuaacaccug uaggaagacu cauuacagca aacccaguua uaacagaauc uacagaaaau     1680

uccaagauga ugcuggaguu agauccccca uuuggugaua guuauauagu uaucggugua     1740

ggugagaaga aaauaacaca ucacuggcac cgaaguggau caacaauagg uaaagcauuc     1800

gaagcaacag uaagaggagc aaaaaggaug gcaguauuag gugacacagc cugggauuuu     1860

ggaucuguag gaggagcacu uaauucauua ggaaaaggca uucaccaaau auuuggagca     1920

gcauuuaaau cauuauucgg uggaauguca ugguuuucac aaauuuugau agguacacua     1980

cuaauguggc uaggacuaaa caccaaaaau gggucaauaa gucuaaugug cuuagcguua     2040

ggaggaguac uaauauuccu aaguacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9108
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  9108
gggagaaagc uuaccaugac auccguaggu auaguagguu uacuauuaac uacugcgaug       60

gccgcagaag ucacaagaag agguucagcu uacuauaugu aucuagaucg uaaugacgca      120

ggagaagcaa uaucauuccc uacaacauua gguaugaaua aauguuauau ccaaauaaug      180

gauuuagggc auauguguga ugcaacaaug aguuaugaau gcccaaugcu agaugaagga      240

guagaaccag acgacguuga cugcuggugu aauacaacaa gcacaugggu aguauacgga      300

acaugucacc auaaaaaagg agaagcucga aggucacgac gagcgguuac auugccaucu      360

cauaguacaa gaaaacuaca gacaagauca cagacauggc uagaaaguag agaauacaca      420

aaacaucuaa uucgaguaga aaacuggaua uuuagaaauc caggcuuugc acucgcagca      480

gcugcaauag cauggcucuu gggaaguuca acaucacaga aaguaauaua ccuaguaaug      540

auauuacuua uagcaccagc cuauaguauu agguguauag gaguaucuaa uagagauuuu      600

guagaaggaa ugagcggagg gacaugggua gauauaguac uagaacaugg aggaugugua      660

acuguaaugg cacaagauaa accaacaguu gacauagaac uaguaacaac aacaguauca      720

aauauggcag aaguaagaag uuauuguuau gaagcgagua uaagugauau ggcaucagau      780

ucaagaugcc cuacacaagg agaagcauac cuagacaaac aaucggauac acaauaugua      840

uguaaaagga cauuaguuga uagagguugg gggaacggau guggguuauu cggaaaagga      900

aguuuaguaa ccugcgccaa auuugcaugu aguaagaaaa ugacagggaa aaguauccaa      960

ccugaaaauc uggaauacag aauuauguua agugugcaug gaagucaaca caguggaaug     1020

auaguaaaug auacgggaca cgaaacagau gaaaauaggg caaaagucga aauuacacca     1080

aacagcccca gagcagaagc aaccuuagga ggauuuggau cauuaggauu agauugcgaa     1140

ccaagaaccg gauuagacuu uagcgaucug uacuauuuga caaugaauaa uaaacauugg     1200

cuaguacaua aagaaugguu ccaugauaua ccauuaccau ggcacgcagg ugcugauacu     1260

ggcacaccac auuggaacaa caaagaagca cucguugaau ucaaagacgc ccaugcaaaa     1320

cgacaaacag uagucguuuu aggaucacaa gaaggagccg uacacacugc auuagcagga     1380

gcauuagaag cagaaaugga uggcgccaaa gggagacuau cgagugguca ucuuaagugc     1440

agacuaaaaa uggauaaauu aagguuaaaa ggaguaaguu acucucuaug cacagcagca     1500

uuuacauuua cuaaaauacc ugcugagaca uuacauggaa cuguaacugu agaaguacaa     1560

uacgcaggga cugauggacc auguaaagug ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccag uaggacgauu aauaacggca aauccaguaa ucacugaauc aacugaaaau     1680

aguaaaauga uguuagaguu agauccacca uucggcgaua guuauauagu aauaggagug     1740

ggcgaaaaaa aaaucacuca ucauuggcac agaagcggca guacauuagg aaaagcauuc     1800

agcacaacau uaaaaggagc ucaaagauua gcagcacuag gugauacagc augggauuuc     1860

ggaucaauug gaggaguauu caauucaaua gggagagcag ugcaucaagu auucgguggc     1920

gcauuuagaa cucuguucgg aggaaugagu uggauaacac aaggacuaau gggugccuua     1980

uuauuaugga ugggaguaaa ugcacgagac cgaucaauag cacuagcauu ucuagcaacu     2040

ggagguguau uaguauuucu ggcaacuaac guccacgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9109
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  9109
gggagaaagc uuaccaugac caguguagga auaguagguu uacuccuuac cacagcaaug       60

gcggcagaag uaacacggag aggaucugca uauuacaugu accuugacag aaaugacgcg      120

ggagaagcaa uaaguuuccc aacaacacua gggaugaaua aaugcuacau ucaaauuaug      180

gaucuaggac auacauguga ugcgacgaug agcuaugaau gcccaauguu agaugaaggu      240

guagaaccag augauguaga cugcuggugu aauacuacau cgaccugggu uguauaugga      300

acaugucauc auaagaaagg agaagcacga agaagcagga gagcaguaac auuacccagu      360

cauucaacaa gaaaauuaca aaccagauca caaacauggc uagaaucaag agaauauaca      420

aaacauuuaa uaagaguaga aaacuggauu uuuagaaauc caggauuugc auuagcugcu      480

gcagcuauag cauggcuucu uggcaguuca accagccaaa aaguuauaua uuuggucaug      540

auuuugcuaa uagcaccagc auauaguaua cgauguauag gaguaucaaa uagagacuuu      600

guugaaggaa ugucuggagg aacauggguc gauguaguac uagaacaugg aggaugugua      660

acaguaaugg cacaagauaa accaacaguu gauauagaau uaguaacaac aacaguaucg      720

aauauggccg aaguaagaag uuauuguuau gaagcaucaa uauccgauau ggcaucagau      780

agcagauguc caacucaagg agaagcauac cuagauaaac aaucagauac ucaauauguu      840

uguaaaagaa cauuaguuga cagaggcugg gguaauggau gcgguuuauu cggcaaaggc      900

ucauuaguaa caugugcaaa guuugcaugu ucaaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaauu uagaauaucg aauaauguua aguguacaug gaucccagca cucaggaaug     1020

auaguuaaug auacagguca cgaaaccgau gagaauagag caaaaguaga gauaacucca     1080

aauaguccaa gagcagaagc uacauuagga ggauuuggua gcuuagguuu agauugugaa     1140

ccacgaacag gauuagauuu cagugaucua uauuaucuaa cuaugaauaa uaaacacugg     1200

uuaguacaua aagaaugguu ucaugauauc ccacuuccau ggcaugcagg agcagauaca     1260

ggaacacccc auuggaauaa uaaagaagca uuggucgaau uuaaagaugc acaugcaaaa     1320

aggcaaacag uuguaguguu aggaucacaa gaaggagcug uacacacagc acuugcuggu     1380

gcacuugagg cagaaaugga uggagcaaaa ggaagacuga gcagcgggca uuuaaaaugu     1440

agauugaaaa uggauaaauu acgccugaaa ggaguaaguu auagucuaug uaccgcagca     1500

uuuacauuua caaaaauacc agcugaaacg cuacauggaa cggucacagu agaaguacaa     1560

uaugcaggua cagauggacc auguaaagua ccagcacaaa uggcaguaga caugcaaaca     1620

cuaacaccag uuggaagauu gaucacugca aauccaguua uaacagaauc aacugaaaau     1680

ucgaaaauga uguuagaguu agacccuccg uuuggugacu cauauauagu aaucggagua     1740

ggagaaaaga aaauaacaca ucacuggcau agaucaggaa guacacuagg aaaggcuuuu     1800

agcacaacau uaaaaggugc ucaaagauua gcagcauuag gcgauacagc uugggacuuu     1860

ggaucaauag gcggaguauu uaacaguaua ggaagggccg uacaucaagu auuuggagga     1920

gcauuuagaa cacucuuugg aggaaugagc uggauaacac aagguuuaau gggagcauua     1980

uuacuuugga ugggaguaaa ugcacgagau cgaaguauag cauuagcauu ucuagcaacu     2040

gguggagucc uaguauuucu agcaacaaac guacaugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9110
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 3 opt

<400>  9110
gggagaaagc uuaccaugac aucaauaggu aucauaggcc uauuacuaac uacagcaaug       60

gcagcagaaa uaacuagaag agguucagca uauuacaugu aucuagauag aagugaugca      120

gguaaagcaa uaaguuuugc aacaacauua ggaguaaaua aaugccaugu acaaauaaug      180

gaucuagguc auauguguga cgcaacaaug aguuaugaau gcccuaugcu agacgaagga      240

guugaaccug augacguaga uuguuggugc aacacaacaa guacuugggu gguauaugga      300

acuugucauc acaagaaagg agaagcaaga cgaucaagac gagccguaac ucuaccaucu      360

cacucaacaa gaaaacuaca aacacgcuca cagacauggu uagaaucaag agaauacacu      420

aaacauuuaa uuaaaguuga aaacuggauu uuucgaaauc cagguuuugc auuaguagcu      480

guagccauag cauggcuacu aggaaguucu acaucucaaa aaguaauaua ucuuguaaug      540

auacuacuaa uagcuccagc auauucaauu agauguauag gaguaagcaa uagagauuuc      600

guagaaggaa ugaguggagg aacaugggua gauguaguau uagaacaugg ugguugugua      660

acaguaaugg cacaagacaa accaacaguc gacauagaau uaguuacaac uacgguaagu      720

aacauggcag aaguuaggag uuauuguuau gaggcaucaa uaagcgauau ggcgucagau      780

ucaagaugcc caacacaagg agaagcauau uuagacaaac aaagugauac gcaauacgua      840

uguaaaagaa cucuaguaga cagggguugg ggaaauggau gcgguuuauu uggaaaaggc      900

agucuaguaa caugcgcaaa auuuacaugu ucaaagaaaa ugacaggaaa aucuauucaa      960

ccugagaauu uagaauaucg aauaauguua ucuguacaug gaucucaaca uucaggaaug     1020

auaggguaug aaacugacga agauagagca aaaguagaag uaacgcccaa uucuccaaga     1080

gcagaagcaa ccuuaggcgg auuuggaagu uuaggacuag auugugaacc aagaacaggu     1140

cuagacuuuu cagaucucua cuaucuaaca augaauaaua aacacuggcu ugugcauaag     1200

gaaugguuuc augauauccc auuaccaugg caugcaggag cagacacagg aacaccucau     1260

uggaauaaua aagaggccuu aguagaauuu aaagaugcac augcgaaaag acaaacagua     1320

guaguuuuag gaagucaaga gggagcaguu cauacagcau uagccggagc acuugaagcg     1380

gaaauggaug gagccaaagg acgucuauuu aguggacacu uaaaaugcag auuaaaaaug     1440

gauaaacuua gacuaaaagg ugucucauac ucucuaugca cagccgcguu uacauuuacc     1500

aagguaccag ccgaaacacu ucacggaacc guaacuguag aaguacaaua ugcaggaaca     1560

gauggaccau guaaaauucc uguacaaaug gcaguagaua ugcaaacauu aacacccgua     1620

gguagauuaa uaacagcaaa ucccguaauu acagaaucaa cagaaaauag uaaaaugaug     1680

uuagaacuag auccuccauu uggagauuca uauauaguaa uaggaguagg ugacaagaaa     1740

auaacacauc auuggcacag aagugguagc acauuaggaa aagcauuuag uacaacacuc     1800

aaaggagcac aaagauuagc agcacuagga gauacagcau gggacuuugg aagcauugga     1860

gguguauuua auaguaucgg uagagcaguu caucaagugu uuggaggagc auucagaaca     1920

cuauuuggag guaugucuug gauuacacaa gguuuaaugg gugcauuauu auuguggaug     1980

ggaguaaaug cacgagaucg uucaauagca uuggccuuuu uagcaaccgg uggaguccug     2040

guguuuuuag caacuaaugu ccaugcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9111
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  9111
gggagaaagc uuaccaugac aucuguaggu auaguaggac uccuuuuaac aacagcaaug       60

gcagcagaag uaacgagaag agguagcgca uauuauaugu auuuagauag aaacgacgcc      120

ggagaagcaa uuagcuuucc uacaacauua ggcaugaaua aauguuauau acaaauaaug      180

gaccuuggac auauguguga ugcaacuaug agcuaugaau gcccuaugcu agaugaggga      240

guagaaccag augacguaga uuguuggugu aacacaacaa gcacaugggu cguuuauggc      300

acaugccauc auaaaaaagg agaggcaaga cguagucgaa gagcaguaac acuaccaucg      360

cauucaacac gaaaauuaca aacuagaagc caaacauggc uagaaucacg agaguacaca      420

aaacauuuaa uaagaguaga aaacuggauc uuuaggaauc cugguuuugc acuagcagca      480

gcagcaaucg cauggcuacu cggaaguagu acgagucaaa aaguaaucua ucuaguaaug      540

auuuuacuaa uagcacccgc uuacucaaua agguguauag guguaucaaa uagagauuuu      600

guggaaggua ugaguggugg aacauggguu gauguaguac uagaacaugg aggauguguc      660

acaguuaugg cucaagauaa accaaccguu gauauagagu uaguuacuac uaccguuucc      720

aauauggcag aaguaagaag cuacugcuau gaagcaagca ucucagauau ggcaucagau      780

ucaagaugcc caacccaggg agaagcauau uuagacaaac aaucagauac acaauaugua      840

ugcaaaagga cauugguuga uagaggaugg gguaauggau gcggauuauu uggcaaagga      900

ucucuaguaa ccugugcuaa auucgcaugc ucuaaaaaaa ugacaggaaa aucgauucaa      960

ccggaaaauc uugaauaccg aauaaugcuu ucaguacaug gaagccaaca uucaggaaug     1020

aucguaaacg auacagguca cgaaacagau gagaauagag cuaaaguaga aauaacucca     1080

aauagcccac gugcugaagc uaccuuagga ggauuugggu cuuuaggauu agauugcgaa     1140

ccacgaacag gauuagauuu cucagaccuc uauuaucuca caaugaacaa uaaacauugg     1200

uuaguccaua aagaaugguu ccaugacauc ccacuaccau ggcacgcagg ugcggacacu     1260

ggaacaccgc acuggaacaa caaagaagca cuagucgaau ucaaggaugc ucacgcaaaa     1320

agacaaacag uaguaguacu gggaucacaa gaaggagcag uacacacagc uuuagcagga     1380

gcauuagaag cagaaaugga cggagcaaaa ggaagauuaa gcagcggaca ucuuaaaugu     1440

agauuaaaaa uggauaaauu gagauuaaaa ggagucucgu auucauuaug cacagccgca     1500

uuuacauuua caaaaauacc cgccgaaaca cuacauggaa caguuacugu cgaaguacaa     1560

uaugcaggga cagacggucc auguaaagua ccugcacaaa uggcaguuga caugcaaaca     1620

cuaacaccag uaggaagauu aaucacagca aacccaguaa uaacagaauc uacagaaaac     1680

aguaaaauga uguuagaauu agauccaccu uuuggcgauu ccuauauagu aauuggagua     1740

ggagaaaaaa aaauuacaca ucauuggcau agaagugguu caacacuagg caaagcuuuu     1800

ucaaccacac uaaaaggugc ucagcgacua gcagcacuug gagauacagc augggauuuc     1860

ggaaguaucg gaggagucuu uaacucaaua ggucgagcag uucaucaagu auucggcgga     1920

gcauuuagaa cacuauuugg uggaauguca uggauaacac aaggacuaau gggagccuua     1980

uuauuaugga ugggaguaaa ugcgagagau agaaguauug cauuagcauu uuuagcaacu     2040

gguggaguac uaguauuuuu agcaacuaac guacaugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9112
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  9112
gggagaaagc uuaccaugag aauuauaaac gcucgaaaag aaaaaaaaag acgaggagca       60

gauacaagug uugguauagu agguuuauug uuaacuacug caauggcagc agaaguaaca      120

agacgaggaa gcgcuuauua uauguaucua gacagaaaug augcaggaga agcgauaagu      180

uucccaacua cauuagguau gaauaaaugu uacauacaaa uaauggauuu agggcauaug      240

ugugaugcaa caaugaguua ugaaugccca auguuagaug agggaguaga acccgaugac      300

guagauugcu ggugcaacac uacaagcaca uggguaguau augguacaug ucaucauaaa      360

aaaggcgaag caagaagaag ccgaagagca guuacacuac caucacauuc aacaagaaaa      420

cuucaaacgc gaucacagac uuggcuagaa ucaagagaau auacaaaaca ucuaauuagg      480

guagaaaauu ggaucuucag aaacccagga uuugcgcuag cagcugcugc uauugcuugg      540

uuauuaggau caucaacaag ccaaaaagua auuuaucuug ugaugauucu acuaauugca      600

cccgcauauu cuauacgcug cauaggagug ucgaaccgag auuuugucga aggaaugucc      660

gggggaaccu ggguagauau cguauuagaa cauggagguu guguuacugu gauggcacaa      720

gauaaaccaa caguagauau agaauuaguu acaacaacag uaagcaauau ggcagaaguu      780

agauccuacu guuaugaagc auccauuagc gauauggcua gugauagcag auguccaaca      840

caaggagaag cuuacuuaga uaaacaaagu gacacacaau auguaugcaa aaggacauug      900

guagaccgag gauggggcaa uggaugcggu uuauucggua aaggcucauu aguaacuugu      960

gcaaaauuug cguguucaaa aaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa     1020

uacagaauaa uguuaucagu acacggaucc caacacucag gaaugauagu aaaugacaca     1080

ggacaugaaa cagaugaaaa cagagcaaaa guagagauaa caccaaauuc accaagagcu     1140

gaagccacau uaggaggauu cggaaguuua gguuuagacu gugaaccaag aacuggauua     1200

gauuuuagcg acuuauauua uuuaacaaug aacaauaaac auugguuagu acauaaagaa     1260

ugguuucaug auauaccauu acccuggcau gcaggagcug auacagggac uccccauugg     1320

aacaauaaag aagcacuugu agaauucaag gaugcacaug cgaaaagaca aacaguagua     1380

gucuuaggca gccaagaagg ggcaguucac acagcauuag caggagcuuu agaagcggag     1440

auggauggcg caaaaggaag auuaagcuca ggccacuuaa aaugcagguu aaagauggac     1500

aaauuaagau uaaaaggagu aaguuauagc cuaugcacag cagcauuuac auucacuaaa     1560

auaccagccg aaacauuaca cggaaccguu acuguugaag uacaauacgc aggaacagac     1620

ggacccugca aaguaccagc ucaaauggcc guagauaugc aaacauuaac ucccguuggg     1680

agacuaauaa cagccaaucc aguaauaaca gaaaguacug aaaauucaaa aaugauguua     1740

gaauuagauc caccuuuugg agacucauac auaguuauag guguagggga aaagaaaaua     1800

acacaccacu ggcacagauc aggcucaacu auugguaaag cauuugaagc uacagucaga     1860

ggcgcgaaaa gaauggcagu acuaggagau acagcauggg auuucggauc aguuggaggg     1920

gcauuaaaca guuuaggaaa aggaauacau caaaucuucg gagcagcuuu caaaucuuua     1980

uuuggaggua ugucuugguu uucucagaua cuaauaggua cauuauuaau gugguuagga     2040

cuaaauacaa aaaauggguc aaucucuuug auguguuuag cacuaggugg aguguugaua     2100

uuuuuaucaa cagcaguaag ugcagacgua gguuguucag uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9113
<211>  2362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9113
gggagaaagc uuaccaugau aauuaacgcu aggaaagaaa aaaaacgaag aggggcagau       60

acauccguag gcauaguagg uuuguuauua acaacagcca uggcagcaga aguaacacga      120

agaggcucag cauacuacau guauuuagau agaaaugaug caggagaagc aauaagcuuu      180

ccaacuacau uaggaaugaa uaaauguuau auacaaauca uggaucuagg acacaugugu      240

gaugcgacaa ugucauauga augcccaaug uuggaugaag gaguugaacc agaugauguc      300

gauugcuggu guaauacaac aaguacaugg guaguauaug gaacaugcca ucauaaaaaa      360

ggagaagcca gaagaagcag acgcgcagua acacucccau cucauaguac aagaaaacua      420

caaacaagau cucaaaccug gcuagagagc agagaauaca caaaacauuu aauaagagua      480

gagaauugga uuuucagaaa uccuggauuu gcccuggcag cagcagcuau ugcauggcua      540

cuugguucga gcacgagcca aaaaguaauc uaucucguua ugauacuacu aauagcucca      600

gcuuacagca uaagauguau uggaguauca aauagagauu uuguagaagg uaugucagga      660

ggaacauggg uagauaucgu uuuagaacau ggaggaugug uaacaguaau ggcgcaagac      720

aaaccaacgg uagacaucga auuaguaaca acgacaguaa guaauauggc agaaguaaga      780

aguuacugcu acgaagcaag uauuucagau auggcaucag acucacgaug uccaacacaa      840

ggagaagcau auuuggauaa acaaucagac acacaauaug uaugcaaaag aacacuagua      900

gacagaggau gggggaacgg auguggacuu uuuggaaaag guucuuuagu aacaugugca      960

aaauuugcgu gcucaaaaaa gaugacagga aaaaguauac aaccagaaaa ucuugaauau     1020

agaauaaugu uaucaguaca cgggagccag cauagcggca ugauaguuaa cgacacugga     1080

cacgaaacag acgaaaacag agcuaaagua gaaauaaccc caaauagucc cagggcagaa     1140

gcaacguuag gaggauuugg aaguuuagga cuagacugug aaccacgaac aggacuagau     1200

uuuagcgauc uauacuacuu aacaaugaac aacaaacacu ggcuagucca uaaagaaugg     1260

uuucaugaca uaccguuacc auggcaugca ggagccgaca ccggaacucc acauuggaau     1320

aacaaagagg cauuaguaga auuuaaagau gcacaugcaa aaagacagac uguaguagua     1380

cuaggaaguc aagaaggagc uguacacaca gcacuugcag gagcauuaga agcagaaaug     1440

gacggagcaa aagggcgguu gucauccgga caucuaaaau gucgauuaaa aauggacaaa     1500

uuaaggcuua aaggaguauc uuacagucua ugcacagcag cauuuacauu uacaaaaaua     1560

ccagcagaaa cauuacaugg uacaguaaca guugaaguac aauaugcagg aacagauggc     1620

ccauguaaag uuccagcaca gauggcagua gauaugcaaa cuuuaacccc aguaggaaga     1680

uugauaacag cuaaucccgu aauaacagaa aguacagaaa auucaaaaau gauguuagaa     1740

cuggauccac cauuuggaga uagcuauaua guaauaggag uaggagagaa aaagauaaca     1800

caucauuggc acagaagugg aagcacaauc ggaaaagcuu uugaagcaac gguaaggggc     1860

gcaaaaagaa uggcaguauu aggagacacc gcgugggauu uuggaagcgu aggaggagca     1920

uuaaauaguu uaggaaaagg aauacaucaa aucuucggag cugcauucaa aucauuauuu     1980

ggugguaugu caugguuuuc acaaauacua auagguaccc uucuaaugug guuaggauua     2040

aauaccaaaa acggcagcau aagcuuaaug uguuuagcau uggguggugu uuuaauuuuu     2100

uuaucuacag caguuucagc agaugucggu uguagcgugg auuuuagcaa gugaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2340

ucuuuucaga gccaccagaa uu                                              2362


<210>  9114
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9114
gggagaaagc uuaccaugau aaaugcaaga aaagaaaaaa aacgaagagg agcagacaca       60

agugucggaa uaguagguuu auuauuaacu accgcaaugg cggcagaagu aacgagaaga      120

ggaucugcau auuacaugua uuuagaucgu aaugaugcug gagaagcaau aucuuuccca      180

acaacacuag gaaugaauaa augcuauauu caaauaaugg accuaggaca uaugugcgau      240

gcaacaauga guuaugaaug uccaauguua gacgaaggag uagaaccaga cgauguggac      300

uguuggugua auacaacuag cacgugggua gucuacggaa caugccauca uaaaaaaggg      360

gaagcaagaa gaaguagacg agcaguaacg uuaccaucac auagcaccag aaaauuacaa      420

accaggucac aaacuuggcu agaaucacga gaauacacaa aacaucuaau uagaguagaa      480

aacuggauau uucgaaaccc gggauuugcc uuagcugcag cagcaauagc auggcuauua      540

ggaucaagua cuucacaaaa aguaauuuau cuaguaauga uacuauuaau agcgccagca      600

uauucaauaa gguguauugg aguuagcaau agagauuuug uagaaggaau gaguggagga      660

acuugggucg auaucguacu agaacaugga ggauguguaa caguaauggc acaagacaaa      720

ccaacaguag auauagaacu aguuacaaca acaguaagua auauggccga agugagaagc      780

uauuguuaug aagccucaau aucagauaug gcaagugacu caaggugucc aacucaagga      840

gaagcauauc uggauaagca aucagacaca caauaugucu guaaaagaac guuaguagau      900

agagguuggg gaaauggaug uggucucuuc ggaaaaggau cauuaguaac augugcaaaa      960

uuugccugca gcaaaaaaau gacaggaaaa agcauacaac cugaaaaccu ggaauauaga     1020

auaaugcucu caguacaugg uagccaacau ucagguauga uagugaauga uacaggacau     1080

gaaacugaug aaaaucgagc aaaaguagaa aucacgccua auaguccgag agcugaagcu     1140

acacuaggag gauuuggaag uuuaggauua gacugugaac caagaacagg auuagauuuu     1200

agugaccucu acuauuuaac aaugaauaau aaacauuggc ucguacauaa agagugguuu     1260

cacgacauac cauuaccaug gcacgcaggu gccgauacag gaacuccgca uuggaauaac     1320

aaagaagcau ugguugaauu uaaagaugca caugcaaaga gacaaacagu aguuguuuua     1380

ggaagccaag aaggagcagu ccacacagcu uuagcaggag cuuuagaggc agaaauggau     1440

ggagcaaaag gaagacuuag cucuggccac cuaaaaugca gauugaaaau ggauaaacug     1500

agacuaaaag gaguaucuua uagucuaugu acagccgcau ucacauuuac uaaaauucca     1560

gcagaaacuu uacaugguac aguaacugua gaaguucaau augcaggaac agauggcccu     1620

uguaaaguac cugcccaaau ggccguagau augcaaacau uaacaccagu aggaagauua     1680

auaacagcaa auccaguaau aacagaaagc accgaaaaua gcaaaaugau guuagaauua     1740

gauccaccau uuggugauag uuauauagua auuggaguag gcgaaaaaaa aauaacacau     1800

cacuggcaua gaucaggguc aacaauaggu aaagcauuug aagccacagu aagaggagca     1860

aaaagaaugg caguuuuagg agauaccgcc ugggacuuug guucaguagg aggagcucuu     1920

aauucuuuag gaaaaggaau acaccaaaua uuuggcgcag cauuuaagag uuuauucgga     1980

ggaaugucau gguuuucaca aauucuaaua ggaacguuau uaauguggcu agguuuaaau     2040

acaaaaaaug ggagcaucuc ccuaaugugu cuugcauuag gaggaguacu uauauuuuua     2100

agcacugcag uaagugcgga cguagguugu aguguagauu uuagcaaaug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  9115
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9115
gggagaaagc uuaccaugaa cgcuagaaaa gaaaaaaaac guagaggugc cgacacaagc       60

guaggaauug uaggguuauu auuaacaaca gcaauggcag cugaaguaac uagaagaggu      120

ucagcauacu auauguacuu agauagaaau gacgcuggag aagcaauaag uuuuccaaca      180

acauuaggua ugaauaaaug uuauauacaa auaauggauc uuggucauau gugcgaugcc      240

acaaugucgu acgaaugucc aaugcuagau gaaggagucg aaccagauga uguagauugu      300

ugguguaaca caacaagcac uuggguagug uaugguacau gucaucacaa aaaaggagag      360

gcaagaagaa guagacgggc uguuacacuu cccagccacu cuacgagaaa acuacaaaca      420

cgaagucaaa cauggcuaga aucaagagaa uacacaaaac accuaauaag aguagaaaau      480

uggauauuua gaaauccagg guucgcucuu gcagcagcag caauagcaug gcuauuagga      540

aguaguacua gucaaaaagu gauauaucua guaaugauac uuuuaauagc gccagccuac      600

agcauaagau guaucggagu aaguaauaga gauuuugucg aaggaauguc uggagguacu      660

uggguagaca uaguacuaga acacggugga ugcgugacug uaauggcaca ggacaaaccc      720

acaguagaca uugaauuagu aacaacaaca guaucaaaca uggcagaagu aagaucauau      780

uguuaugaag caaguauaag ugauauggca ucagauaguc gauguccaac acaaggagaa      840

gcuuauuuag auaaacaaag ugauacgcaa uacguaugua aaagaacgcu aguagacagg      900

gguuggggaa acggaugugg uuuauucggu aaggguucau uaguaacaug ugccaaauuu      960

gcauguagua aaaaaaugac uggaaaaucu auacaaccug aaaauuuaga auacagaauu     1020

auguuaucgg uucauggaag ccagcacagc ggaaugauag uuaaugauac aggacaugaa     1080

acggaugaaa auagagcaaa aguagaaaua acaccaaaca guccaagagc agaagcaaca     1140

uuaggaggau uuggaagcuu aggauuggau ugugagccga gaacaggauu agauuuuagc     1200

gaucuauacu auuuaacaau gaauaacaaa cauugguuag uacauaaaga augguuucac     1260

gauauuccac uaccguggca ugcaggagca gauacaggua caccgcauug gaauaacaaa     1320

gaggccuuag uugaguucaa agaugcucau gcuaaaagac aaacggucgu aguacuagga     1380

ucacaagaag gagcaguaca cacagcacua gcaggagcuc uugaagccga aauggauggu     1440

gcgaaaggac gauuaucaag uggacauuua aaaugcagac uaaaaaugga uaaauugaga     1500

uuaaaaggag uaaguuacag cuuauguaca gcugccuuua cuuucacaaa aaucccagcu     1560

gaaacauuac auggaacagu uacaguugaa guucaauaug caggaacaga ugguccaugu     1620

aaaguaccag cacaaauggc aguagauaug caaacauuaa cuccaguagg aagauuaaua     1680

acugccaauc cuguaaucac agaaaguacc gaaaauucaa aaaugauguu agaauuggau     1740

ccgccauuug gagauucuua uauagucaua gguguaggug aaaaaaaaau aacacaucau     1800

uggcauagaa gugguucaac aauagguaaa gcauucgaag caacaguaag gggagcaaaa     1860

agaauggcag uacuaggaga uacagcuugg gacuuuggua gcgucggagg ugcuuuaaau     1920

agcuuaggaa aagguauuca ucaaauauuu ggugccgcau uuaaaaguuu auucggaggg     1980

augaguuggu uuagucaaau acucauugga acucuuuuga uguggcuagg auuaaacaca     2040

aaaaauggaa guauuucacu aauguguuua gcauuaggag gaguauuaau auuccuaucu     2100

acagcagucu cagcugaugu aggaugcuca gucgacuuua guaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  9116
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9116
gggagaaagc uuaccauggc aagaaaagaa aagaaaagga gaggagcaga cacaagugua       60

ggaauaguag guuuauuacu gacuacggca auggcagcag aaguuacaag aaggggaagc      120

gcauauuaua uguauuuaga uagaaaugau gcaggagaag caauaucauu cccaaccaca      180

uuaggaauga acaaauguua cauacaaaua auggaccucg gacauaugug cgaugccaca      240

augucauaug aaugcccgau guuagacgaa ggaguagaac cagaugaugu agauuguugg      300

uguaauacua cuagcacaug gguaguauac ggaacaugcc aucauaagaa aggagaagcu      360

cgaagaucua gaagggcugu aacuuuaccc ucacauucaa cacguaaauu acagacaaga      420

agccaaacau ggcuagaauc aagggaauau acaaaacacu uaauuagagu agaaaauugg      480

auauucagaa auccaggauu cgcguuagca gccgcagcga uagcuugguu auuaggauca      540

aguacaucuc aaaaaguaau auaucuaguc augauacuac uaauagcgcc agcuuacagu      600

auaagaugca uaggaguaag uaacagagac uuuguagaag gaaugucagg aggcacaugg      660

guagacauag uacuagaaca ugguggaugu guaacaguga uggcacaaga caaaccaaca      720

guagauauag aauuaguaac aacgacagua agcaauaugg cagaaguaag gaguuauugu      780

uaugaagcau caauuucaga uauggcaagc gauucaagau gucccacuca aggugaagca      840

uaucuagaua aacaaagcga uacgcaauau guauguaaaa gaacuuuagu agaucgaggc      900

ugggguaacg gaugcgguuu auuuggaaaa gggagcuuag ucacaugugc aaaauucgca      960

uguucgaaga aaaugacugg aaaaaguaua caaccugaaa aucuagaaua cagaauuaug     1020

uuaucaguac auggaaguca acauagugga augauuguaa acgauacagg ucaugaaacg     1080

gacgaaaauc gcgcaaaagu agaaauaaca ccuaauaguc cacgagcaga agcuacuuua     1140

ggggguuucg ggucauuagg auuggacugu gaaccaagaa cagguuuaga cuuuagugac     1200

cuauauuacu uaacaaugaa caauaaacac ugguugguac acaaagaaug guuucaugac     1260

auaccauuac ccuggcaugc aggagcagac acuggaacuc cacauuggaa uaauaaagaa     1320

gcgcuaguag aauuuaaaga ugcacaugca aaaagacaaa cuguaguugu ccuaggauca     1380

caagaaggag caguacauac agcauuagcu ggagcauuag aggcagaaau ggauggagca     1440

aaaggaagau uauccagugg gcaucuaaaa ugcagguuaa aaauggauaa acuuaggcua     1500

aaaggaguau cuuacucacu auguaccgcu gcauucacau uuacaaaaau uccugcagaa     1560

acauuacaug gaacagugac aguagaagug caauaugcug gaacugacgg uccauguaaa     1620

guuccagcac aaauggcagu agauaugcaa acauuaacuc cuguagguag acuuauaaca     1680

gcaaacccag uaauaacaga aaguacagaa aauucaaaaa ugaugcuaga auuagaucca     1740

ccauucggag auagcuauau agugauugga guaggugaaa agaaaauuac acaccacugg     1800

cacaggagug guaguacaau aggaaaagcg uuugaagcaa cuguaagagg ugcaaaaaga     1860

auggcugucu uaggugacac ugcaugggau uuugguagug uuggaggagc uuuaaauuca     1920

cuaggaaaag guauacacca aauauucgga gcagcauuua aaagucuauu uggcgguaug     1980

uccugguuuu cacaaauauu aauaggaacg uuauuaaugu gguuaggacu aaacacgaaa     2040

aauggaagca uaucauugau guguuuagcu cuaggaggag uuuuaauauu ucuuucaaca     2100

gcagucucag cagauguugg auguucagua gacuuuagua aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  9117
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9117
gggagaaagc uuaccaugag aaaagaaaaa aaaagacgag gagcagacac aucagucgga       60

auuguaggac uacuuuuaac aacagcaaug gcagcagagg uuacuaggag agguucagcc      120

uauuauaugu auuuagauag aaacgaugcu ggcgaagcaa uuaguuuccc cacaacauua      180

ggaaugaaua aauguuauau acaaauuaug gauuuggguc auaugugcga cgcaacaaug      240

aguuaugaau gccccaugcu ggaugaaggg gucgaaccag acgauguaga uuguuggugu      300

aacacaacau caaccugggu aguauacggg acaugucauc acaaaaaagg agaagcgaga      360

cgaagcagaa gagcaguuac acuaccaagu cacucaacac gaaaacuaca aacacgauca      420

caaacauggu uagaaaguag agaguacaca aaacaucuua uucgaguaga gaauuggaua      480

uuuaggaauc caggauuugc auuagcagca gcagcaauag caugguuauu agguucauca      540

acaagucaaa aaguaauaua ccuuguaaug auacuacuua uagcaccugc uuauaguaua      600

agauguauug gaguaucaaa uagagacuuu gucgaaggaa ugaguggagg cacgugggua      660

gauauaguau uagaacacgg aggaugugua acagucaugg cacaagacaa accuacagua      720

gacaucgaac ucgucacuac cacaguauca aauauggcag aaguaagauc auacuguuau      780

gaagccagua uaagugauau ggcaagugac ucaagauguc caacacaagg ugaagcauau      840

cucgauaaac aauccgauac ucaauaugua uguaaaagaa cauuaguaga uagaggaugg      900

ggcaauggau guggacuuuu uggaaaaggg ucacuaguaa caugcgcaaa auuugcaugu      960

aguaaaaaaa ugacagguaa aucaauacaa ccagaaaacc uugaauaucg aauaauguua     1020

aguguucaug gaagccaaca cucgggcaug aucgugaacg acacagggca cgaaaccgau     1080

gagaauagag caaagguaga aauaacacca aauagcccaa gagcagaagc uaccuuagga     1140

ggauucggau cauuaggcuu agauugugaa ccaagaacag gguuagacuu uucagaucua     1200

uacuaucuaa ccaugaauaa uaaacauugg cuaguucaca aagaaugguu ucaugauaua     1260

ccauuaccau ggcacgcagg agcagacacg ggaacaccac auuggaacaa caaagaagca     1320

uuaguggaau uuaaagaugc acacgcaaaa agacaaacug uugugguacu aggaagucag     1380

gaaggagcag uacauacagc auuagcggga gcgcuagaag cagaaaugga uggagcaaaa     1440

ggaagacuau caucugguca cuuaaaaugu agauugaaaa uggauaaguu aagauuaaag     1500

gggguuagcu auucucuaug uacagcggcc uucacuuuua caaaaauucc ggcagaaaca     1560

cugcauggaa caguaacagu agaaguccag uaugcaggua cagacggacc auguaaagua     1620

cccgcccaaa uggcaguaga uaugcaaaca uugacuccag uaggaagacu cauaaccgcu     1680

aauccgguua uaacagagag uacagaaaac ucaaaaauga ugcuagaacu cgauccgcca     1740

uucggagauu cguauauagu uauagguguu ggagaaaaaa aaauaacaca ccauuggcau     1800

agaucaggcu ccacaauagg aaaagccuuu gaagcuaccg uaaggggagc aaaaagaaug     1860

gcaguauuag gagauacugc augggauuuc ggaucaguug gaggagcacu aaauagcuua     1920

ggaaaaggaa uacaucaaau auuuggagca gcauuuaaau cuuuguuugg aggaauguca     1980

ugguucaguc aaauauuaau aggaacguua uuaauguggu uaggucugaa uaccaaaaau     2040

ggaagcauua guuuaaugug uuuagcauua ggaggaguau uaauauuucu aucgacagcu     2100

guaucagcag auguggguug uucaguagau uuuuccaaau gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2220

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  9118
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9118
gggagaaagc uuaccaugaa agaaaaaaaa agaagaggag cagauacauc aguaggaauc       60

guaggauuac uauugacaac agcuauggca gcagaaguaa caagaagagg aucggcauau      120

uauauguauu uagaucgcaa cgaugcugga gaagcaauuu cauucccaac cacuuuggga      180

augaauaaau gcuacauaca aauaauggau uugggacaua ugugugacgc gacuauguca      240

uacgaguguc cgaugcuaga ugaaggagua gaaccugaug acguagauug uugguguaau      300

acuacaagca cuuggguagu cuaugguacg ugccaucaua aaaaagguga agcucgaaga      360

agcagaagag caguuacuuu accuucacac ucaacaagaa aauuacaaac gagaagucaa      420

acaugguuag aaucaagaga auauacaaaa cauuuaauaa gaguugaaaa uuggauauuu      480

agaaauccug guuuugcauu agcugcggca gcaauagccu gguuauuagg aucaucaacu      540

agucaaaaag uaauuuauuu aguaaugauu uuauuaauag caccugcgua uaguauaaga      600

uguauaggag uaaguaauag agauuuugua gaagguaugu caggaggaac auggguagac      660

auaguauugg aacauggggg gugcguaaca gucauggcuc aagacaaacc aacaguagac      720

auagaauuag uuacaacaac uguauccaau auggcagaag uaagaagcua uuguuacgaa      780

gcuucuauaa gcgacauggc uucagauuca agauguccua cacaagguga agcauauuua      840

gacaaacaaa gugauaccca auacguaugc aaaagaacau ugguggauag aggauggggu      900

aauggaugcg gauuauuugg aaagggauca cuaguaacau gugcaaaauu ugcauguagc      960

aaaaaaauga caggaaaaag uauacaaccg gaaaaucuag aauauagaau aauguuaucg     1020

guacacggua gucaacauuc aggaaugaua guaaaugaua caggacauga aacagaugag     1080

aacagagcua aaguugaaau uacaccgaau ucuccaagag cagaagcaac gcuaggagga     1140

uuuggaaguc uagguuuaga uugcgaacca agaacagguu uagacuucag ugacuuauac     1200

uacuuaacaa ugaacaauaa acauugguua guacacaaag aaugguuuca ugauauacca     1260

cuaccauggc augcaggagc ggauacugga acaccacacu ggaacaauaa agaagcauua     1320

guagaauuua aagaugcaca ugcaaaaaga caaacaguag uagugcucgg uucacaagaa     1380

ggagcagugc acacagcauu ggccggagca cuagaagcag aaauggaugg agcaaaagga     1440

agacuaucau caggacaucu aaaaugcagg cuaaaaaugg auaagcuuag auuaaaagga     1500

guaucauauu cacugugcac agcagcuuuc acauucacca aaauuccugc agaaaccuug     1560

cacgguaccg uaacaguaga aguacaguau gccgggacag auggaccaug uaaaguacca     1620

gcgcaaaugg cggucgauau gcagacucua acaccagugg gaagguuaau aacagcuaau     1680

cccgugauaa cagaaucaac agaaaauagu aaaaugaugu uagaacuaga uccuccauuu     1740

ggagauucau acauaguaau aggaguugga gaaaaaaaaa uaacacauca cuggcauagg     1800

agcggcucaa ccauuggaaa agcauucgaa gcuacaguaa gaggcgcaaa acguauggca     1860

guauuaggag auacagcaug ggauuuugga ucaguuggag gugcauuaaa cucacucgga     1920

aaaggaauac aucaaauauu cggagcugcu uucaaauccu uguucggagg uaugaguugg     1980

uuuucucaaa uauuaauagg aacacuauug augugguuag gauuaaauac aaaaaacgga     2040

agcauaucau uaauguguuu agcauuaggg ggugucuuaa uuuucuuauc aacagcagug     2100

ucagcagaug ucggguguag cguugauuuu aguaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9119
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9119
gggagaaagc uuaccaugga aaagaaaaga agaggagcag auacaucagu gggaauugua       60

gggcuauuau uaacaaccgc aauggcagca gaaguaacaa gaaggggaag cgcauacuau      120

auguauuuag accguaauga cgcuggagaa gcgauuuccu uucccacaac auuaggaaug      180

aauaaaugcu acauacaaau aauggacuua ggacauaugu gugaugccac aaugucauac      240

gaauguccaa ugcuugacga aggaguagaa ccagaugacg ucgacuguug gugcaauaca      300

acaucuacuu ggguuguuua ugggacuugu caucacaaga aaggagaggc cagacgaagu      360

cguagagcag uaacuuuacc aagccacuca acaagaaaac uacagacuag aagucaaacg      420

ugguuagaau caagagaaua uacaaaacau uuaauacgag uggaaaacug gauauucaga      480

aacccuggau ucgcauuggc agcagcugca auugcauggu uauuaggauc aaguacauca      540

caaaaaguaa uauauuuagu uaugauauua cuaauugcac ccgcuuauuc aauaagaugu      600

auaggaguau cuaacagaga uuuuguugaa ggcaugagcg gaggaacaug gguagauaua      660

guauuggaac augggggaug cguuacagua auggcacaag auaaaccaac aguggauaua      720

gaauuaguca caacaacagu uaguaacaug gcagagguac gaucuuauug uuacgaagca      780

aguauaagug auauggcauc ggauaguaga uguccuacac aaggagaggc uuauuuagac      840

aaacaaagug auacacaaua uguuuguaaa agaacacuug uugauagagg uugggguaac      900

gguugcggau uauuugguaa aggcucacug guuacgugug caaaauucgc auguagcaag      960

aaaaugacug gaaaaaguau acaaccagaa aaucuagaau auagaauaau guuaagugua     1020

cacggaagcc aacauucagg aaugauagua aaugacacag gacacgaaac agacgaaaau     1080

agagcaaaag uugaaaucac uccuaauuca ccaagagcag aagcaacacu aggagguuuu     1140

ggaucucuag gccucgacug ugaaccacgc acaggauuag acuuuucaga cuuauacuau     1200

cuuacaauga auaacaaaca uuggcuagua cauaaagaau gguuccacga cauuccacua     1260

ccauggcacg caggagcaga cacaggaaca ccacauugga acaacaaaga agcacuugua     1320

gaauuuaaag augcccaugc caaaagacaa acaguaguag uauuaggauc acaagaaggc     1380

gcuguacaua cugcccuagc cggcgcacua gaagccgaaa uggauggcgc aaaaggaaga     1440

cuaucaagug ggcauuuaaa auguagacuu aaaauggaua aacugcgacu aaaaggagua     1500

ucguacucau uauguacggc agcuuuuaca uuuaccaaaa uaccagcaga aacacuacac     1560

ggaacuguaa cgguggaagu ucaauacgcu ggcacagaug gaccuuguaa aguaccagca     1620

caaauggcag uagacaugca aacuuuaaca ccaguaggaa ggcuaauaac agccaacccg     1680

guaauaacug aaaguacaga aaauucaaaa augaugcuag aauuagaucc uccguucgga     1740

gauagcuaca uaguaauugg uguaggagaa aagaaaauaa cccaucauug gcaccgaagu     1800

ggaucuacaa ucggaaaagc auucgaagca acaguaagag gagccaaaag aauggcugua     1860

cuuggggaua cagcauggga uuuuggauca guagggggag cgcucaauag uuuaggaaaa     1920

ggaauacauc aaauauuugg agcagcuuuu aaaaguuuau uuggaggaau gaguugguuc     1980

ucacaaauau uaauagguac ccuacuaaug ugguuaggac uuaauacuaa aaauggaagu     2040

auaucauuga uguguuuagc auuaggugga guucuaauau uuuuaucaac agcaguaagu     2100

gcagauguag guugcucagu agacuucagc aaaugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  9120
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9120
gggagaaagc uuaccaugaa aaaaaggaga ggagcggaua caucaguagg aauuguagga       60

uuacuguuaa cuacugcaau ggcagcagaa guuacaagaa gaggaagugc cuauuauaug      120

uauuuggaca gaaacgacgc aggagaagca auaucauuuc caacaacauu aggaaugaau      180

aaauguuaua uacaaauaau ggauuuagga cauaugugug augccacaau gucuuaugaa      240

uguccuaugu uagacgaagg aguagaaccu gaugacguug auuguuggug caacacaaca      300

aguacuuggg uaguauaugg aacaugccau cacaaaaaag gggaagcaag gagaucaaga      360

agggcaguua cauuaccauc ucacucuacg agaaaauuac agacaagauc acaaacgugg      420

uuagaaagua gagaauauac aaaacacuug auacgagucg aaaauuggau cuuccggaau      480

ccagguuuug cgcuagcagc agcagcaaua gcauggcuau uaggaaguuc aacaagucaa      540

aaaguaauau acuuagugau gauuuuguua auugcaccug cguacaguau aagauguaua      600

ggagugucua auagagacuu uguagaagga augucaggcg gaacaugggu agauauugua      660

cuagaacacg gaggaugcgu gacaguaaug gcacaagaca aacccacagu agauauagaa      720

uuaguaacca caacaguaag caauauggca gaaguaagaa gcuauuguua ugaggcaagu      780

auaucagaua uggcaaguga uucaagaugu ccaacucagg gagaagcuua uuuagauaaa      840

caaagcgaca cacaauaugu auguaaaaga acacuuguag acagaggaug gggaaacggg      900

ugcgguuuau uuggaaaagg uucauuagua accugugcaa aauuugcaug cucuaaaaaa      960

augacuggaa aaaguauaca acccgaaaau uuagaauaua gaauaaugcu aaguguacac     1020

ggaucacaac auucagggau gauaguaaau gauacgggac augaaacaga ugaaaauaga     1080

gcaaaaguag aaauaacucc caacagucca agagcagaag cgacacuagg uggguuuggu     1140

aguuuagggc uggacugcga accaagaacg gguuuagacu uuucagauuu guauuaucua     1200

acuaugaaca auaaacauug guuaguacau aaagaguggu uucacgacau uccgcuucca     1260

uggcaugccg gugcagacac aggaacacca cauuggaaca auaaagaagc auuaguagaa     1320

uuuaaagaug cacaugcaaa aagacagacc guaguagucu uaggaaguca agaaggagcg     1380

guacacacug cacuagcagg agcacuagaa gcagaaaugg acggagcaaa aggaagacug     1440

ucaaguggac accuaaaaug uagguuaaaa auggauaaac uaagauuaaa agguguuuca     1500

uauucacuau guacagcagc auuuacauuu acgaaaauac cagcagaaac auuacacggu     1560

acaguaacag uagaaguaca guaugcagga acagauggac cuugcaaagu accugcacag     1620

auggcuguag auaugcaaac acuuacacca guaggcagac uaauaacagc aaauccagua     1680

auuaccgaaa guaccgaaaa uucaaaaaug auguuagaac uugauccacc uuuuggagac     1740

agcuauauag uaauaggggu gggagaaaaa aaaauaacac accauuggca uagaagugga     1800

aguaccauag ggaaagcauu ugaagcaaca guuaggggag caaaaagaau ggcgguauua     1860

ggggacacag cuugggacuu uggaaguguu gguggagcau uaaauucauu aggaaaagga     1920

auacaucaaa uauuuggagc agcauuuaaa ucauuauuug gagggaugag uugguuuagu     1980

caaauauuaa uaggaacacu auuaaugugg uuaggguuaa auacuaaaaa uggcaguaua     2040

agcuuaaugu guuuagcacu agggggagug cuaauauuuc uaaguacagc cguaagugca     2100

gacgucggau gcaguguuga uuuuuccaaa ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  9121
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9121
gggagaaagc uuaccaugaa aagaagaggu gcagacacga guguuggaau agugggauug       60

uuacuaacaa cagcaauggc ugcagagguu accagaagag gaucugcaua uuauauguau      120

uuagacagaa acgaugccgg ugaagcaaua aguuucccaa cgacucuagg aaugaauaaa      180

ugcuacauac aaauuaugga cuuaggacau augugcgaug caacaaugag cuaugaaugc      240

ccaauguuag augagggagu agaaccagac gauguagauu guugguguaa uacaacauca      300

acuuggguag uuuacgguac uugccaucau aaaaaaggag aagcaagaag aucaagaaga      360

gcaguaacau ugccuaguca uucaacacgc aaacuccaaa caagaucaca aacaugguug      420

gaaucaagag aauacacaaa acauuuaaua agaguagaaa auuggaucuu ucgaaaucca      480

ggauuugcac uagcagcagc agcaauagca ugguuauuag gaaguucuac aucacaaaaa      540

guaauauacc uagucaugau acucuugaua gccccugcau auucuauaag augcauagga      600

guuaguaaua gagauuucgu ugaaggaaug uccggaggaa cuuggguuga uauagucuua      660

gaacacggag gauguguaac uguaauggca caagauaaac caacuguaga uauagaauua      720

guuacaacaa caguaucuaa uauggcagag guacgaucau acuguuauga ggccaguaua      780

agugauaugg cauccgacuc acgaugucca acacaaggag aagcuuaccu ugauaaacaa      840

agugauacgc aauauguaug uaaaagaaca uuaguagauc gaggaugggg caauggcugc      900

ggauuauuug gcaaaggauc cuuaguaaca ugugcaaaau ucgcauguag caaaaaaaug      960

acaggaaaau caauacaacc agaaaauuua gaauauagaa uaaugcuauc aguacaugga     1020

ucacaacaua gcggaaugau aguuaacgau accggacaug aaaccgacga aaauagagca     1080

aaaguagaaa uaacaccaaa cagcccaagg gcagaagcaa cauuaggcgg auuuggaucc     1140

uuagguuuag acugugaacc acgcacuggu uuggauuuua gugauuuaua cuaccuuaca     1200

augaacaaua aacauugguu aguccacaaa gaaugguuuc augauauacc auuaccaugg     1260

caugcuggug cagauacugg gaccccacau uggaauaaua aagaagcauu aguagaguuu     1320

aaagaugcac augcaaaaag acaaacaguc gucguccuag ggagucaaga aggagcagua     1380

cacacagcac uagcaggagc ccuugaagca gaaauggacg gagcaaaagg aagauuaagu     1440

ucaggacauu uaaagugucg ccuuaaaaug gacaaauuaa gacuaaaagg agucaguuau     1500

ucgcuaugua ccgcagcguu cacguuuaca aaaauuccgg cagaaacucu acaugguacu     1560

guuacuguag agguccaaua ugcaggcaca gacggaccau guaaaguacc agcucaaaug     1620

gccgucgaca ugcaaacacu cacuccagua ggaagauuaa uaacagcaaa uccaguaauu     1680

acagaaucaa cagaaaauag uaaaaugaug uuagaauugg auccacccuu uggagacagu     1740

uauauuguaa uaggagucgg agaaaaaaaa auaacacauc auuggcaucg aucaggauca     1800

acgauaggaa aagcauuuga ggcaaccguc agaggagcua aaagaauggc aguucuagga     1860

gacaccgcau gggauuucgg aucagucgga ggggcacuaa auucucucgg uaaagggaua     1920

caucaaauau uuggagccgc auuuaaaucc uuauucggag guaugaguug guuuucacaa     1980

auccuaauag gaacauugcu aauguggcug ggacuaaaua cgaaaaaugg uaguauauca     2040

uugaugugcu uagcucuagg agguguauua aucuuucuua gcacagcagu uucggcagac     2100

gucggaugua guguagauuu caguaaauga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9122
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9122
gggagaaagc uuaccaugag aagaggagcc gacacuucag ugggaauugu aggauuauua       60

uuaacaaccg caauggcagc agaaguuaca agaaggggau cagcauauua uauguaucuu      120

gauagaaaug acgcagguga agccauuagu uuccccacaa ccuuagguau gaauaaaugc      180

uauauacaaa uaauggauuu aggacauaug ugugaugcaa ccaugaguua cgaaugccca      240

auguuagacg aagggguuga acccgaugau guagauugcu gguguaacac uacaaguaca      300

uggguaguau auggcacaug ucaucacaaa aaaggugaag caagaagauc aagacgagca      360

guuaccuuac cuagccauag uacacgcaaa uuacaaacaa gaucacagac uugguuagaa      420

aguagggaau auaccaaaca cuuaauaaga guugaaaauu ggauauuuag aaaucccgga      480

uuugcauuag cagcagcagc aauagcgugg uuacuaggca gcucaacuag ucaaaaagua      540

auauaucuag uaaugaucuu auuaauagcu ccagcauauu caauaagaug uauaggagug      600

agcaauagag auuuuguaga aggaaugagu ggagguacau ggguagacau aguacuagaa      660

cacggaggcu guguaacagu cauggcacaa gauaaaccaa caguugauau cgaauuggua      720

acaacaacag uaagcaacau ggcggaagua agaagcuacu gcuaugaagc uaguauaagc      780

gacauggcaa gugauucaag auguccuaca caaggagaag cguaucuaga uaaacaaagu      840

gauacacaau auguuuguaa aagaacauua guagauagag gauggggaaa uggaugugga      900

uuguuuggua aagggucauu ggucacuugc gcuaaauuug cauguaguaa aaaaaugaca      960

ggaaaaagca uucaaccaga aaaccuagaa uauagaauaa ugcuuagugu ccauggauca     1020

cagcauucag guaugauagu aaaugacaca ggacaugaaa cagacgaaaa uagagcaaaa     1080

guagaaauaa caccgaacag cccaagagca gaagcuacuu uaggaggauu uggaaguuua     1140

ggacuagacu gugaaccaag aacaggcuua gauuuuagug acuuauauua uuugacgaug     1200

aauaauaaac acugguuagu acacaaagaa ugguuccaug auauaccauu gccauggcau     1260

gcaggugcag auacaggaac accacacugg aauaacaaag aggcccuggu agaguucaaa     1320

gaugcacaug caaaaaggca aacaguugua guauuaggau cucaagaagg ugcaguucac     1380

acugcccuag ccggagcauu agaagccgaa auggauggag cuaaaggaag auugaguagc     1440

ggacaucuua aaugcagauu aaaaauggau aaauuaagac ucaaaggugu aagcuauagu     1500

uuauguacag cagccuuuac auuuacuaaa auacccgcag aaacacuaca uggaacaguu     1560

acaguugaag uacaauacgc aggaacggau gguccaugua aaguaccugc ucaaauggca     1620

guagacaugc agacacuaac gccgguaggu agauuaauaa cagcgaaucc cguuauaacu     1680

gaaucaacag aaaauucaaa aaugaugcua gaauuagauc caccuuuugg agacagcuau     1740

auaguaauag gaguaggaga aaaaaaaaua acacaucacu ggcauagauc aggcagcaca     1800

auugguaaag cuuuugaagc uacaguucga ggagcaaaaa gaauggcagu acuaggagau     1860

acagccuggg auuuuggaag cguaggugga gcuuuaaacu cuuugggaaa agguauacau     1920

caaauuuuug gagcugcuuu uaagaguuug uuugguggaa ugucgugguu uagucaaaua     1980

uugauaggca cauuacucau gugguuagga cuaaauacaa aaaacggauc aauaagucua     2040

auguguuuag cacuaggagg aguauuaaua uuuuuaucua cagcaguaag cgcagacguu     2100

ggauguucag uagauuucag uaaaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  9123
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9123
gggagaaagc uuaccaugag gggagcagau acaucaguag gaauaguagg auuauuauua       60

acaacagcaa uggcagcaga aguuacaagg aggggaagug ccuacuacau guacuuagau      120

agaaacgaug caggagaagc aauuaguuuu ccuacaacau uaggaaugaa uaaaugcuau      180

auacaaauaa uggacuuagg ucauaugugc gacgcaacua ugucuuacga augcccaaug      240

uuagaugaag gaguagaacc agacgaugua gacuguuggu guaauaccac aaguacgugg      300

guaguauaug gaacuuguca ucacaagaaa ggagaagcaa gaagauccag gagagcagua      360

acuuuaccau cacacucaac gagaaaauua cagacaagaa gccaaacaug guuagaauca      420

agagaauaua caaaacacuu aaucagagua gaaaauugga uauuucgaaa uccaggauuc      480

gcacuugcgg cagcugcuau agcauggcuc uuaggaaguu cuaccagcca aaaaguaauu      540

uauuuaguaa ugauacuuuu aauagcaccc gcauauucaa uaagguguau cggaguuucu      600

aauagagauu uuguggaagg aaugucaggc ggaacuuggg uggacaucgu uuuagaacac      660

ggaggaugug uuacaguaau ggcucaagau aaaccaacug uagauauaga auuaguaacu      720

acgacagugu caaauauggc agaaguaaga ucuuauuguu augaggcuuc aaucagugac      780

auggcaucag auaguagaug uccaacacaa ggagaagcau auuuagacaa acaaagugac      840

acacaauaug ucuguaaaag gacauuagua gacagagggu ggggaaaugg auguggacuc      900

uuuggaaaag gaucucuagu aacuugcgcu aaauuugcau guucaaagaa aaugacagga      960

aaaucaauac aaccagaaaa ucucgaauau agaauuaugu uaucaguaca uggcucacaa     1020

cauaguggaa ugauaguuaa ugauacagga caugaaacag augaaaacag agcaaaagua     1080

gaaauuacac cuaacucacc uagagccgaa gccacacuag gugguuuugg aaguuuagga     1140

cuagauugug aaccaaggac aggucuagau uuuagcgacu uauauuauuu aacaaugaau     1200

aauaaacauu gguuggucca uaaagaaugg uuccacgaua uuccauuacc uuggcaugca     1260

ggagcagaua caggaacacc acauuggaau aacaaagaag cauuaguuga auuuaaagau     1320

gcacaugcua aaagacaaac uguaguugua cuuggauccc aagaaggagc aguucauaca     1380

gcauuagcgg gugcacuuga agcagaaaug gauggagcaa aagguagacu aucaagcgga     1440

caucugaaau gucgacuaaa aauggauaaa cugagauuga aaggaguuag uuauaguuua     1500

ugcacagcag cauucacauu uacaaaaauc ccagcagaaa cauuacaugg aacaguaacu     1560

guagaaguac aauaugccgg aacugaugga cccuguaaag uucccgcaca gauggcggua     1620

gacaugcaga cauuaacucc aguagguaga uuaauaacgg caaauccugu cauaacggaa     1680

ucuacagaaa auucaaaaau gauguuggaa uuagacccac cauuuggaga uuccuacaua     1740

guuauaggag ucggugaaaa gaaaauaaca caucauuggc acaggagcgg aaguaccauu     1800

gguaaggcuu uugaagcaac aguaagagga gcaaaaagaa uggcaguuuu gggagauaca     1860

gcaugggauu ucgguagugu uggaggagcu cuuaauucuu uaggcaaagg aauacaucaa     1920

auauucggag cagcuuuuaa aagucuauuc ggagggauga guugguuuag ccaaauacua     1980

auuggaacgc uacugaugug guuaggacuc aacacuaaaa acgggagcau uaguuuaaug     2040

ugccuagcau uagguggagu acuaauauuu cuaucaacag caguuucagc cgaugucggc     2100

uguucaguag auuuuuccaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9124
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9124
gggagaaagc uuaccauggg ugcagauaca ucaguuggaa uaguaggacu gcuauuaaca       60

acagcaaugg cggcagaagu aacaagacgg ggaucagcuu auuacaugua ucuagauaga      120

aaugaugcag gagaagcaau aucauuccca acgacacuag gaaugaacaa auguuacauc      180

caaauaaugg accuaggaca uaugugcgac gccacuauga gcuaugaaug cccaaugcua      240

gaugaaggag uagaaccgga ugauguugac uguuggugua auacgacauc aacuugggua      300

guauacggaa cuugucauca uaagaaagga gaagcaagac gaaguagacg agcaguaaca      360

cuaccaucac acaguacaag aaaauuacaa acaagaaguc agacauggcu ugaaagcaga      420

gaauauacaa aacauuuaau aagaguagaa aauuggauuu uuagaaaucc agguuucgcc      480

uuagcugcag cugcgauagc auggcucuug ggaucuagca cuucacaaaa aguaauauac      540

uuaguaauga uuuuacuaau ugcaccagca uauucaauca gauguauugg agucagcaau      600

agggacuuug uagaaggcau gucaggugga acguggguag auaucguacu agaacacgga      660

gggugcguaa caguaauggc acaggauaaa ccaacgguag acauagaauu aguaacgaca      720

acgguaucaa auauggcaga aguaagaagu uacuguuacg aggcaucuau uagugacaug      780

gcuucagacu cacgaugucc aacacaagga gaagcauauu uagauaaaca aagugauacg      840

caauacguau gcaaaagaac auuaguagac agaggguggg guaauggaug cgguuuauuu      900

ggaaaaggau cauuaguaac augugcaaaa uuugcauguu cuaagaaaau gacaggaaaa      960

aguauccaac cagagaauuu agaauacagg auuauguuaa guguacacgg aucacaacau     1020

ucagggauga uaguaaauga cacaggacau gaaacugaug aaaaucgagc aaaaguagaa     1080

auaacuccca auucaccaag agcugaagcu acauuagggg gauuuggauc acuaggacua     1140

gauugugaac cgagaacagg auuagauuuu agugauuuau auuauuuaac aaugaacaau     1200

aaacacuggu uaguacauaa agaaugguuu caugacauac cauuaccgug gcacgcaggg     1260

gcagauacug gaacaccaca uuggaauaau aaagaggcac ugguugaguu caaagacgcu     1320

cacgcaaaaa gacaaacagu agucguguua ggcucucagg aaggagcagu acauaccgcg     1380

uuagcaggcg cacuagaagc agaaauggau ggcgcuaaag gcagacuuag cagcggacac     1440

uugaaaugca gacuaaaaau ggacaaauua cgguuaaaag gcgugaguua uucauuaugu     1500

acagccgcau ucacauucac aaaaauaccu gcugaaacuu uacaugggac agugacggua     1560

gaaguacagu augcggggac agacggaccu uguaaaguac cugcacaaau ggcaguagau     1620

augcaaacuu uaacaccagu aggaagauua auaacagcaa auccaguaau uacagaaagu     1680

acagaaaaua guaaaaugau gcuagaauua gacccaccuu uuggcgacuc auauauaguc     1740

auaggaguag gugaaaagaa aauaacacac cauuggcaca ggaguggauc uacaauagga     1800

aaagcauuug aagccacagu aagaggagcc aaaagaaugg caguacuagg agacacagcu     1860

ugggauuuug gaagcgucgg aggagcacua aauaguuuag gaaaaggaau acaucaaaua     1920

uucggagcag ccuucaaauc auuauuuggu gguauguccu gguuuaguca aauauuaaua     1980

ggaacauuau uaaugugguu aggacuaaau acuaaaaacg guaguaucuc acuuaugugu     2040

uuagcccugg guggaguauu aauauuuuua ucaaccgcug uaucagcuga uguuggaugu     2100

ucaguagauu ucucaaaaug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9125
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9125
gggagaaagc uuaccauggc agauacaucc guaggaauag uaggacuuuu auuaacaaca       60

gcaauggcag cggaaguaac aagaagaggu ucagccuauu acauguauuu ggauaggaau      120

gacgcaggag aagcaauuag cuucccuaca acacuuggga ugaacaaaug uuauauacaa      180

aucauggacc uaggacauau gugugaugca acuaugaguu acgaaugucc aaugcuugau      240

gagggaguag aaccagacga cgucgauugu uggugcaaua cuacaaguac uuggguagua      300

uaugguacau gucaucauaa aaaaggagaa gcaagacgcu caaggagagc aguaacauua      360

ccuagucacu caacaagaaa acugcaaaca cgaagucaaa cauggcucga gagccgagaa      420

uauacaaaac aucuaauuag aguagagaac uggauauuca gaaauccagg auucgccuua      480

gcggcagcag caauagcaug guuacuagga aguucaacau cacaaaaagu aauauaucua      540

gucaugauuu uauuaauagc uccugcauau ucuauacgau guauaggagu caguaauagg      600

gauuuuguag aaggaaugag uggagguacc uggguagaua uuguauuaga acacggaggc      660

uguguaacug uuauggcaca agauaaaccu acaguagaua uagaacuagu aacgacaaca      720

gugagcaaca uggcugaagu gagaucauau uguuaugaag cuagcauaag cgauauggcc      780

agugauucua gauguccaac ccaaggagaa gcauacuuag auaagcaauc ugauacccaa      840

uauguaugca aaagaacacu aguagaccga ggauggggca auggaugcgg ucuauucgga      900

aaaggaagcu uagugacaug ugcaaaauuu gcauguucca aaaaaaugac aggcaaaagc      960

auacaaccag aaaauuuaga auacaggaua augcuaucag uacauggauc ucaacauagc     1020

ggaaugauag uaaaugauac aggacacgaa acugacgaaa auagagccaa aguagagauc     1080

acacccaaca guccaagagc agaagcaacg cuaggaggau uuggaagccu cggauuagau     1140

ugugaaccaa gaacaggacu agauuucagc gaucuuuacu auuuaaccau gaacaacaaa     1200

cauugguuag uacauaaaga gugguuucau gauauaccgu uaccuuggca ugcaggagca     1260

gauacaggaa caccacacug gaauaauaaa gaagcccuug uagaauuuaa agacgcacau     1320

gcaaaaagac aaacgguagu uguauuaggu agucaagaag gagcaguaca uacagcacua     1380

gcuggugcau uagaagcaga aauggacgga gcaaaaggaa gauuaagcag cggacaucua     1440

aagugcagac uaaaaaugga uaaacuucgc cuaaaaggag uaucauacuc auuauguacu     1500

gccgcuuuua cauucaccaa aauaccugcu gaaacuuuac acggaacagu cacuguagaa     1560

guacaauaug cagguacaga ugguccaugu aaagugcccg cucaaauggc aguagauaug     1620

caaacacuaa caccuguagg aagauuaaua acagcaaacc cuguaauaac agaaucaaca     1680

gaaaacucga aaaugaugcu cgaacuagac ccaccguuug gagauaguua cauuguaauu     1740

gguguaggag aaaaaaaaau uacacaucau uggcaccguu cagggaguac uauaggaaaa     1800

gcauuugaag caacuguaag aggagcaaaa agaauggcag uauuagguga uacagccugg     1860

gacuucggua guguaggagg agcauugaac ucccuaggaa aaggcauuca ucaaauauuu     1920

ggagcagcau uuaaaaguuu auuuggagga augucauggu uuagucaaau auuaauaggc     1980

acgcuacuua ugugguuagg acuaaauaca aaaaacggaa guauuagccu aaugugcuua     2040

gcuuuagggg gcguguuaau uuuuuugagc acagcaguua gcgcagacgu aggauguagu     2100

guugauuuuu caaaaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9126
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9126
gggagaaagc uuaccaugga cacuagugua ggaauaguag gucuacuacu aacaacagca       60

auggcagcag aaguuacucg aagagguagc gcauauuaua uguauuuaga uagaaaugac      120

gcaggagaag cuauaaguuu cccaacaaca cuaggaauga acaaauguua cauucaaaua      180

auggaccugg gacauaugug cgaugcaacc augucuuacg aauguccuau gcuugacgaa      240

ggaguagaac cugaugaugu ugacuguugg uguaauacaa ccagcacuug gguaguauau      300

ggaacauguc aucauaaaaa aggggaagca agacgaucaa gaagggcagu gacauugcca      360

agucacucaa cuagaaaauu acaaacuaga ucacaaacau gguuggaauc aagagaauac      420

acaaaacauu uaauuagagu ggaaaauugg auauuccgaa acccaggauu cgcauuagcg      480

gcagcagcaa uagcuugguu auugggaagc ucaacgaguc aaaagguuau auaccuagug      540

augauacuau uaauagcucc agcauauucc auaagaugca uaggaguaag uaaccgagac      600

uuuguagaag gaaugagugg aggaacaugg guagauauag uauuggaaca uggaggaugu      660

guaacaguaa uggcacaaga uaaaccaaca guagauauag aacuaguuac aacaacaguu      720

ucaaauaugg ccgaaguaag aucguacugu uaugaggcua gcauaucaga cauggcaucu      780

gauucaagau guccaacuca aggcgaagca uacuuagaua aacagaguga cacgcaauau      840

guaugcaaaa gaacacucgu ggauagagga ugggguaacg gguguggccu guucggcaaa      900

gguagucuag uaacaugugc uaaauuugcu uguucaaaaa aaaugacagg aaaaucaaua      960

caaccggaaa accuagaaua cagaauaaug uuaagugucc acgggaguca acauucagga     1020

augauuguua augauacagg ucaugagacu gaugaaaaua gagcaaaagu agagaucaca     1080

ccaaauucac cacgagcaga agcaacacua ggugguuuug gaaguuuagg auuagauugu     1140

gaaccaagaa caggauuaga uuuuucagac uuguauuacu uaacaaugaa uaauaaacac     1200

ugguuagucc acaaagaaug guuucaugau auuccacugc cauggcaugc aggagcagau     1260

acaggcacac cacacuggaa uaacaaagaa gcauuaguug aauuuaaaga ugcacacgcu     1320

aaaagacaaa caguagucgu auuaggaagu caagaaggag ccguucacac agcacuagca     1380

ggugcauuag aagcugaaau ggacggagcu aaagguagau uaucaagugg acaccuaaaa     1440

ugcagacuga aaauggacaa auuaagacua aaaggaguaa guuauagcuu auguacagcg     1500

gcauuuacau uuacaaaaau accggcagaa acauuacaug guacaguaac uguagaagua     1560

caauacgccg gaacagaugg accaugcaaa guaccugcuc aaauggccgu cgauaugcaa     1620

acccuaacac cgguuggaag acucauaaca gcaaauccag uaauaacaga gucaacagaa     1680

aauaguaaaa ugauguuaga auuagaccca ccauuuggug acucguacau agugauagga     1740

guuggugaaa aaaaaauaac acaccauugg cacagaagug gaagcacuau uggaaaagca     1800

uuugaagcaa cuguuagagg cgcaaaaaga auggcaguuc uuggagauac agcaugggau     1860

uuuggaagug uuggaggagc auuaaacagu uuaggaaaag gcauacauca aauuuuugga     1920

gcagcuuuua aaucacuauu uggaggaaug ucaugguuuu cacaaauauu aauugguacu     1980

uuacuaaugu gguuaggacu aaauacuaaa aaugguagua uaaguuuaau gugucuagca     2040

uuaggcggag ucuuaaucuu cuuaucaaca gcaguaagcg cagacguagg guguagugua     2100

gacuucucaa aaugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  9127
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9127
gggagaaagc uuaccaugac cagcguugga auaguagguu uacuauuaac aaccgcaaug       60

gcagcagaag ugacaagaag aggaagugca uacuauaugu acuuagaucg uaacgaugca      120

ggugaagcua uaucuuuucc aacaacguua ggaaugaaua aauguuacau acaaauaaug      180

gacuuaggac auauguguga cgcaacuaug ucauaugaau guccaauguu agaugaagga      240

guugaaccag acgacguaga uuguuggugu aauacuacca guacaugggu aguauaugga      300

acaugccauc auaaaaaagg agaggcuaga agaaguagga gagcaguaac auuaccaucu      360

cauucaacaa gaaaacuuca aacacgauca caaaccuggc uagaaagccg agaauacaca      420

aaacaucuaa uacgagugga aaauuggaua uuuagaaauc caggauucgc acuagcugca      480

gccgcuaucg cauggcuacu agguaguuca acaucacaaa aaguaauuua cuuaguaaug      540

auccuacuca uugccccggc auauucaaua agaugcaucg ggguuagcaa cagagacuuu      600

guugaaggca ugucgggagg cacaugggua gauauuguac uugagcacgg agggugcgua      660

acuguuaugg cucaagauaa accaacaguu gacauagaac uaguaacaac aacaguauca      720

aacauggcgg aaguaagaag uuauugcuau gaagcaagca uaucugacau ggcaucagau      780

ucuagauguc caacacaagg agaagcuuau uuagauaaac aaucagacac acaauacgua      840

uguaaaagaa cauuaguaga cagaggcugg ggaaauggau guggauuguu uggaaaagga      900

ucauugguaa caugugccaa auucgcaugu aguaaaaaaa ugacuggaaa aaguauccaa      960

ccagaaaauu uagaauaucg aauuauguua agcguccaug guucccaaca uucagguaug     1020

auaguaaacg auacaggaca cgagacugau gaaaauagag caaaagugga aauaacucca     1080

aauaguccaa gagcagaagc aacuuuaggu gguuuuggau cccucggauu ggauugugaa     1140

ccaagaaccg gauuagacuu cagugaucua uacuacuuaa caaugaauaa uaaacacugg     1200

cuuguccaca aagaaugguu ccacgacauc ccauuaccau ggcacgcagg cgcagauaca     1260

ggcaccccac auuggaauaa uaaagaagca cuaguagaau ucaaagacgc acaugcaaaa     1320

agacaaacgg uaguaguauu aggaagucaa gaaggagcug uacauacagc ccuagcugga     1380

gcacuagaag cagaaaugga cggagcaaaa gguagauuaa guaguggaca ccugaaaugc     1440

agauuaaaaa uggauaaacu aagguuaaaa ggaguuagcu auucuuuaug uacugcagca     1500

uuuacauuca cuaaaauacc agcagaaacc uuacauggaa cagugacagu agaaguacaa     1560

uaugcaggaa cugauggacc auguaaagua ccagcacaaa uggcaguuga uaugcaaaca     1620

uuaacuccag uagggagacu uauaacagca aauccaguaa uaaccgaauc aacagaaaau     1680

agcaaaauga uguuagaauu agacccaccu uucggcgaca gcuacauagu cauaggaguu     1740

ggugaaaaaa aaauaaccca ucauuggcau agaaguggaa gcacaauagg caaagcauuu     1800

gaagcaacug ucagaggggc caaaagaaug gcaguucuag gagauaccgc augggacuuc     1860

ggaaguguag gaggagcacu aaacaguuua ggaaaaggaa uacaucagau auuuggggca     1920

gcauucaaaa gucuauuugg gggcaugagc ugguuuucac aaauuuuaau uggaacauug     1980

uuaauguggc uaggauuaaa uacaaaaaau gguagcauaa gcuuaaugug ucuagcucua     2040

ggaggaguac ugauuuuuuu aagcacagcu guuagugcug auguagggug uucuguagau     2100

uuuucaaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9128
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9128
gggagaaagc uuaccaugac aaguguuggu auaguggggu uauuauuaac gacugcaaug       60

gcagcagcag uaacaagaag aggaucagcu uauuauaugu auuuagacag aaaugaugca      120

ggggaagcaa ucucauuucc caccacucua gguaugaaua aauguuauau ucaaauaaug      180

gaucucgguc auauguguga ugcuacuaug aguuaugaau guccaauguu agaugaagga      240

guugaaccag augaugucga cuguuggugc aauacaacaa guacuugggu aguauaugga      300

accugucauc auaagaaagg agaagcuaga cguaguagga gagcaguaac acuuccaagu      360

cauucaacaa gaaaacuaca aacuagaagc caaacauggu uagaaaguag agaauauaca      420

aaacaccuaa uaagaguaga aaacuggauu uuuagaaacc caggauuugc ccuggcagcu      480

gcugcuauag ccugguuacu gggauccagu acaucacaaa aaguaauaua cuuaguaaug      540

auauuauuga uagcucccgc uuauagcaua agauguauug gcgucucuaa uagagauuuc      600

guagaaggga ugaguggugg gacaugggua gauauaguau uagaacacgg agguugcgua      660

acaguaaugg cacaagacaa accaacagua gauauagaau uagugacaac aacagugagc      720

aauauggcug aaguacgauc auauugcuau gaagcaucaa ucucagauau ggcuucagac      780

ucaagauguc caacgcaagg agaagcauau cuagacaaac aaagugauac acaauacgua      840

uguaaaagaa cauuaguaga uagagguugg gguaaugguu guggacuauu uggaaaaggc      900

uccuuaguua caugugcuaa auucgcuugc uccaagaaaa ugacaggaaa aucaauacaa      960

ccugaaaauc uugaauacag aauaaugcua aguguacaug gaagccaaca uagugggaug     1020

auaguaaaug auacgggaca ugaaacugau gaaaauagag caaaaguaga aauaacucca     1080

aauagcccaa gagcagaagc aacuuuaggc ggauucggca gccuuggauu agauugugaa     1140

ccaagaacag gcuuagauuu uagcgacuua uauuacuuaa caaugaauaa caaacauugg     1200

uuaguccaua aagaaugguu ucacgauaua ccauuaccau ggcaugcggg ugcugauacc     1260

gguacaccac auuggaauaa caaagaagca uugguagagu ucaaagacgc acacgcaaaa     1320

aggcaaacag ucguagucuu gggaagccaa gaaggagccg uacauaccgc auuagcagga     1380

gcacuagaag cagaaaugga cggagcaaaa ggaagauuaa gcaguggaca uuuaaaaugu     1440

cgauuaaaaa uggauaaacu uagauuaaaa ggagucucau auucacuuug uacagcagca     1500

uuuacuuuua caaaaauacc ugcagaaaca uuacauggga cagugacagu ggaaguacaa     1560

uaugcaggaa cagauggacc auguaaagua ccagcucaaa uggcuguuga uaugcaaaca     1620

cuaacaccag uaggacgacu aauaacugcu aauccaguaa uaacagaauc aacugaaaau     1680

agcaaaauga ugcuagaacu ugacccgccu uuuggugaca guuauauagu uauagguguc     1740

ggugaaaaga aaaucacaca ucacuggcau aggagugggu caacaauagg gaaagcguuu     1800

gaagcaacag ucaggggugc aaaaagaaug gcuguacuag gagauacagc augggauuuu     1860

ggaaguguag gaggggcauu gaauagucua ggaaaaggua uacaucaaau auuuggggca     1920

gcauuuaaaa gucuauuugg cggaauguca ugguuuucac aaauucuaau aggaacuuua     1980

cuuauguggc ucggauugaa uacgaaaaau ggaucaauca gcuuaaugug uuuagcuuua     2040

ggaggugugc uaauauuuuu aaguacagcc guaucagccg auguaggaug uucuguagau     2100

uuuaguaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9129
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9129
gggagaaagc uuaccaugag aggagcagau acaaguguug ggauaguagg acugcuucua       60

acuacugcaa uggccgcugc gguaacgaga cgaggaagug cauauuacau guauuuagac      120

agaaacgaug caggagaagc uauaaguuuu ccaacaacgc uagguaugaa caaaugcuac      180

auacagauaa uggaucuagg acacaugugu gacgcaacua ugucauacga augcccaaug      240

uuagaugaag guguagaacc agaugauguc gacuguuggu gcaacacaac uucgacaugg      300

guaguauaug gaacauguca ucauaaaaaa ggagaagcac gccgaucaag aagggcaguc      360

acauuaccau cacacaguac aaggaaauua caaacaagaa gucaaacaug gcuugaauca      420

agagaauaua caaaacacuu aauuagggua gaaaauugga uuuucagaaa uccaggauuu      480

gcauuagcag ccgcagcaau agcuugguua uuaggaagua guacaagcca aaaaguaauu      540

uauuuaguua ugauacuuuu aauagcucca gcauauucaa uacgauguau agguguauca      600

aacagagauu uuguugaagg aaugagugga ggaacauggg uagauauagu ucuagaacau      660

ggagguugug uaacaguaau ggcacaggac aaaccaacag uagacauaga auuaguaaca      720

acaacaguuu caaauauggc agaaguaaga ucuuauuguu acgaagcaag uauaagcgau      780

auggcuagcg auucacgaug ucccacacaa ggagaagcuu aucuagauaa acaaucagau      840

acacaauacg uauguaaacg aacauuggua gacagaggau ggggaaaugg auguggauua      900

uuuggaaaag guagccuagu aacaugcgca aaauucgccu guaguaaaaa aaugacagga      960

aaaucaauuc aaccagaaaa cuuagaauau agaauaaugc uauccguuca cggcucgcaa     1020

cauucaggga ugauuguaaa ugauacagga caugaaacag augaaaacag ggcuaaggua     1080

gaaauaacuc ccaauucacc aagagcugaa gcaacauuag gcggguuugg aucacucggu     1140

cuagacugug aaccacgaac uggauuagau uuuucagauc ucuacuaucu gacuaugaau     1200

aauaaacauu gguuagucca uaaagaaugg uuucacgaua uaccacuacc cuggcaugca     1260

ggugcagaua cuggaacacc ucacuggaau aauaaagaag cucuuguaga guuuaaagau     1320

gcacaugcaa aaagacaaac aguaguagua uuaggaaguc aagaaggagc aguacacaca     1380

gcauuagccg gugcucuaga agcagaaaug gacggagcaa aaggcagacu cucaagugga     1440

cauuuaaaau gucgauuaaa aauggacaaa uuaagguuaa aagguguaag uuacagucua     1500

uguacagcug cauuuacauu uacaaaaaua ccugcugaaa cgcuccaugg aacagucaca     1560

guugaagucc aauaugccgg aaccgauggc ccauguaaag ucccugcaca aauggcggua     1620

gacaugcaaa cauuaacacc aguugguaga uuaauaaccg caaauccggu uauaacagaa     1680

aguacagaaa auuccaaaau gauguuagaa uuagauccac cauuuggaga cuccuauaua     1740

guaauuggag uaggagaaaa aaaaauaaca caucauuggc acagaucagg uucaacaaua     1800

ggaaaagcgu ucgaagcgac agugagagga gcaaaaagaa uggcuguauu aggagauaca     1860

gcuugggacu uuggguccgu aggcggugca cuuaauucac uuggaaaagg cauacaucaa     1920

auuuucgggg cggcuuuuaa aucacuuuuc ggaggaaugu caugguuuuc acagauauua     1980

auaggaacau uauuaaugug guuaggacuu aauacaaaaa auggaagcau uaguuuaaug     2040

ugccuagcgc ugggaggugu acuuauauuu cuuucaaccg caguaagugc ugauguaggg     2100

uguucuguag acuuuuccaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9130
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9130
gggagaaagc uuaccaugag aggacuguca ucgagaaaaa gaagaagcca ugauguccua       60

acaguucaau uucuaauauu agggauguua cuaaugacgg gaggaguaac uagacgugga      120

ucagcauauu auauguaccu agauagaaac gaugcuggug aagcaauauc auuuccaaca      180

accuuaggaa ugaauaaaug uuacauucaa auuauggauu ugggucauau gugcgaugca      240

accauguccu augaaugucc aauguuagac gaaggagugg aaccagauga uguagauugu      300

uggugcaaua caacgucaac uuggguagua uauggaacuu gucaucauaa gaaaggagaa      360

gcaagaagau cuagacgagc aguaacauua ccaucacauu caacuagaaa acuacaaaca      420

agaagccaaa caugguuaga gaguagagaa uacaccaaac aucuaauaag gguugaaaau      480

uggauauuuc guaauccugg uuucgcauua gcagcagcag cuauagcaug guuauuagga      540

agcaguacau cacaaaaagu aauauacuua gucaugauau uacuaaucgc accagccuau      600

ucaauaagau guauaggagu uaguaauaga gauuuugugg aagguaugag ugguggaaca      660

uggguugaca ucguacuaga acauggaggg uguguuacug uaauggcaca agauaaaccg      720

acaguugaua ucgaauuagu gacaacaaca guaucuaaua uggcagaagu aagaagcuau      780

uguuaugaag cuagcauauc cgauauggca ucagacagca gaugucccac acagggagaa      840

gcauaucuug auaaacaguc agacacacag uauguaugca aaagaacauu aguagacaga      900

ggguggggaa acggaugugg uuuauuugga aaaggaucau uaguaaccug ugcuaaauuu      960

gcaugcagca agaaaaugac uggcaaaagu auacaaccgg aaaaucuaga auaccgaaua     1020

augcucagug uucacggauc acaacauuca gguaugauug uaaacgauac uggacaugaa     1080

acagaugaaa acagagcaaa agucgaaaua acuccaaaca guccacgagc agaagcuacu     1140

uuaggcgguu uuggaucccu uggauuagau ugugagccac gaacuggauu agauuucagu     1200

gauuuauauu auuuaacaau gaauaauaaa cacugguuag uacacaaaga augguuccau     1260

gauauaccau uaccuuggca ugcaggagcu gauacaggga caccacacug gaauaacaaa     1320

gaagcccuag uagaauuuaa agaugcacau gcgaaacgac aaacaguugu ugucuuagga     1380

ucucaagaag gugcaguaca uacagcauua gcaggagccc uagaagcaga aauggacggu     1440

gcuaaagggc gauuaucuag cggacauuua aaauguagau uaaaaaugga uaaauuaaga     1500

cuaaaaggug uuagcuauag uuuauguaca gccgcauuca cauucacuaa aauaccugca     1560

gagacucuac auggaacagu aacuguagaa guacaauaug cagguacuga cggaccuugc     1620

aaaguuccag cacaaauggc aguugacaug caaacacuaa cuccaguagg aagauuaaua     1680

acggcgaacc caguuauaac agaaucaacu gaaaauagca aaaugaugcu ugaacuagau     1740

ccaccauuug gagacucaua uauaguaaua ggaguaggcg aaaagaaaau aacacaucac     1800

uggcauagaa gugguagcac uauugguaaa gcauuugaag caacuguaag aggugcaaaa     1860

agaauggcag uauugggaga uacugcaugg gauuuuggau caguaggcgg ggcacuaaau     1920

ucacuuggga aaggaauaca ucaaauauuu ggagcagcau uuaaaucuuu auuuggaggc     1980

augucauggu ucucacaaau auugauugga acccuacuua uguggcuagg uuuaaacacu     2040

aaaaauggau caauuucauu gaugugcuua gcauugggag gaguauuaau auucuuaagc     2100

acagcaguaa gugcagacgu aggaugcagu guugacuuua guaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  9131
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9131
gggagaaagc uuaccaugag aggauuaagu agcagaaaaa gaagaagcca ugacguguua       60

acaguacaau uucugauacu gggaaugcua cuaaugacug gagcaguaac uagaagagga      120

agcgcauauu auauguacuu agauagaaau gaugcuggug aagcuauauc cuuuccaacc      180

acauugggaa ugaauaaaug uuauauacaa auuauggacu uaggacauau gugugaugcu      240

acaaugucau augagugucc uaugcuagau gaaggaguag aaccagauga cguagauugu      300

ugguguaaua caacgucaac auggguagua uauggaacuu gucaucauaa gaaaggagaa      360

gcaagaagau caaggagagc ugugacacua ccuucacaua gcacaagaaa acuucaaacc      420

agaagccaaa cauggcucga aucaagagaa uauacuaaac aucuaauacg uguugaaaau      480

uggauauuua gaaacccagg auucgcacuu gccgcagcag caauagcaug guuauugggu      540

ucgaguacau cucagaaagu aauauacuua guaaugauuc uauuaaucgc accagcauau      600

ucaauaagau gcauaggagu gagcaaccga gauuuuguug aaggcaugag uggaggaacg      660

uggguugaua uaguucugga acacggagga uguguuacag uaauggcaca agacaaacca      720

acaguagaca uagaauuggu caccacaaca guaaguaaua uggcagaagu aagaagcuac      780

uguuacgagg ccaguauuag ugauauggca ucagauucca gauguccuac acaaggagaa      840

gcauauuuag acaaacaauc agauacacaa uauguaugua aaaggacauu aguugacaga      900

ggauggggaa auggaugcgg auuauuuggu aaaggaucau uagugacaug ugcuaaauuu      960

gcaugcagua agaaaaugac aggaaaaagc auacaaccgg aaaaucuuga guauaggaua     1020

auguuaagug uacacggauc acaacauucu ggaaugauug uaaaugauac uggacaugaa     1080

acagaugaaa aucgagcaaa aguagaaaua acucccaauu caccaagagc ugaggcaaca     1140

cuaggaggau uugggagcuu aggauuagau ugugaaccua ggacuggacu ugauuuuagu     1200

gaucuauauu auuuaacgau gaauaauaaa cauuggcuag uacauaaaga augguuucau     1260

gacaucccau uaccauggca ugccggggca gauacaggaa caccacacug gaauaacaaa     1320

gaagcguugg uagaauuuaa agaugcacau gcaaaaagac aaacaguugu aguauuagga     1380

agccaagagg gagcaguaca uacugcacuu gcaggagcau uagaagcaga aauggauggu     1440

gcaaaaggaa gacuaucauc agggcauuua aaauguagac uuaaaaugga uaaauuaaga     1500

cuaaaaggcg uaagcuauuc auuauguaca gcagcauuua cuuucacaaa aauaccagca     1560

gaaacauuac auggaacagu aacaguggaa guacaauaug cgggaacaga uggaccaugu     1620

aaaguuccag cacaaauggc aguugauaug caaacauuaa caccaguugg aagacuaaua     1680

acagcaaauc caguaauaac ugaauccaca gaaaacucaa aaaugaugcu ugaacuagau     1740

ccaccauuug gagauaguua uauagugauu ggcguugggg agaagaaaau aacacaucau     1800

uggcauagau caggaucaac aauaggaaaa gcauuugaag caacuguaag aggagcaaaa     1860

agaauggcag uucuaggaga uacagcaugg gauuuuggaa gcguuggagg agcgcuaaau     1920

agcuugggga aagggauuca ccaaauauuu ggagcugcau uuaaaucauu auuuggagga     1980

augaguuggu uuagccaaau acuaauagga accuuauuga ugugguuagg auuaaauacu     2040

aaaaacggaa guauaaguuu aaugugucua gcacuagggg gaguuuuaau auuuuuaagu     2100

acagcaguau cagccgaugu aggcuguagu guagauuuuu caaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  9132
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9132
gggagaaagc uuaccaugag aauuauaaau gcaaggaaag agaaaaaaag aagaggugca       60

gauacaagcg uugguauagu aggacuacua uuaacaacug caauggcggc agaaguaaca      120

agacgugguu cagcuuauua cauguauuua gauagaaacg augcuggaga ggcaauauca      180

uuuccuacaa cuuuaggaau gaauaaaugc uauauacaaa uaauggauuu aggacauaug      240

ugugacgcaa caaugaguua ugaaugcccu augcuagaug aaggaguuga acccgacgau      300

guagauuguu ggugcaauac uacaaguaca uggguuguau auggaacaug ucaucacaaa      360

aaaggagagg cuagaagauc aagaagagca guaacauuac cuucacacag cacacgaaaa      420

cuacaaacaa gaucacaaac augguuagaa ucaagagaau acacaaaaca ucuaauuagg      480

guagaaaauu ggaucuuuag aaauccuggc uuugcacuug cagcagcagc aauagcuugg      540

uuauuaggua gcaguacauc ucaaaaagua auauauuuag uaaugauacu acuaaucgca      600

ccagcauaca gcauaaggug cauaggagua ucuaauaggg auuuuguaga aggaauguca      660

ggaggaacau ggguugacau uguacuagaa cauggugguu guguaacagu aauggcgcaa      720

gauaaaccaa cagucgauau agaacuagua acaacaaccg uaucaaauau ggcugaaguu      780

agaucauauu guuaugaagc aucaaucagc gauauggcau cagacagcag augcccaacu      840

caaggagaag cuuauuuaga uaagcaauca gauacucaau acguauguaa aagaacacua      900

gucgauagag gaugggguaa cggauguggu uuauucggca aaggaucauu aguuaccugu      960

gccaaauuug cauguucaaa aaaaaugacu ggaaaaagca uacaacccga gaauuuagaa     1020

uauaggauaa uguugucugu ccauggaucu caacacucag guaugauagu uaaugauaca     1080

ggacacgaaa cagaugaaaa uagggcuaaa guagaaauaa cacccaauag uccucgagcu     1140

gaagcaacac ucggaggauu uggaagucua ggacuagacu gugaaccaag aacaggacua     1200

gacuuuagug aucuauauua uuuaacaaug aacaacaaac auuggcuagu acauaaagaa     1260

ugguuucacg acauaccucu accuuggcac gcaggcgcug auacaggaac accacacugg     1320

aacaauaaag aagcacuagu ugaauuuaaa gaugcucacg caaaaagaca aacagucguc     1380

guacuaggaa gucaagaagg agcuguacau accgcgcuag ccggcgcuuu agaagccgag     1440

auggacggag caaaaggaag auuaucaucg ggacacuuaa aauguagauu aaaaauggac     1500

aagcuaagau uaaaaggagu cucauauuca cuaugcacag cagcauuuac auucacaaaa     1560

auuccugcgg aaacacuaca uggaacagua acuguugaag uacaauaugc aggaacagau     1620

ggacccugca agguuccugc acaaauggcu guagauaugc aaacacuaac accaguagga     1680

agacuaauaa cagcaaaccc aguaauaaca gaaucuacag aaaauucaaa aaugauguug     1740

gaauuagauc caccauuugg ggauaguuau auaguaauag guguaggaga aaaaaaaaua     1800

acacaucacu ggcauagaag uggcuccacg auaggaaaag cauucgaagc aacaguacga     1860

ggagcaaaaa gaauggcagu acuaggagac acagcauggg auuucggauc cguuggagga     1920

gcauugaauu cauuagguaa agguauccac caaauauuug gagcggcauu uaaaucguua     1980

uucggaggaa ugaguugguu uucucaaauc cuaauuggua cacuacucau gugguuaggu     2040

uuaaacacaa aaaaugguuc aaucucauua augugcuuag cgcuuggagg aguacuaaua     2100

uuucucagua cagcugugag ugcugacgua gguuguugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9133
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9133
gggagaaagc uuaccaugau uauuaaugca agaaaagaaa agaaaagaag aggugccgau       60

acaagcguag gaauaguagg acuacuacuu acaacagcaa uggcagccga aguaacaaga      120

cgcgguucug cuuauuauau guaucuagac agaaaugaug cuggagaagc cauuaguuuc      180

ccaacaacau uaggaaugaa uaaauguuac auacaaauaa uggaccuagg acacaugugu      240

gacgcaacaa ugucauauga augcccaaug uuagacgaag guguugaacc agacgaugua      300

gauuguuggu guaauacaac aucaacaugg guuguuuaug gaacaugcca ucauaagaaa      360

ggcgaggcua gaagaucaag acgagcaguc acauuaccua gucauaguac aagaaaauua      420

caaacaagau cucagacaug gcuugaauca agagaauaua caaaacacuu aauuagaguu      480

gaaaauugga ucuuuagaaa cccugguuuu gcacuagccg cagcugcaau agcuuggcua      540

uuaggaucau cuacaucaca aaaaguaaua uauuuaguca ugauucuacu aauagcacca      600

gcauauucaa ucagaugcau aggagugagc aauagagauu uuguggaagg caugucugga      660

gggacauggg uagauauugu auuagaacau ggagggugcg uaacuguuau ggcacaggau      720

aaaccaacag uagacauaga auuaguuaca acaacaguuu caaauauggc ggaaguuaga      780

aguuacuguu augaagcauc aauuucagau auggcaucag auaguagaug uccaacacaa      840

ggggaggcuu auuuagauaa acaaagugac acucaauaug uauguaaacg aacauuagua      900

gauagaggau ggggcaacgg uugugguuua uuuggcaaag gaaguuuagu aacaugugca      960

aaauuugcgu guaguaagaa aaugacaggc aaaucaauac aaccagaaaa cuuagaauau     1020

cgaaucaugc uuaguguaca cggcucacaa cauucaggaa ugauuguaaa ugacacugga     1080

caugagacag augaaaacag agcaaaagug gaaauuacac cuaacucacc aagagcagaa     1140

gcuacguuag gagguuucgg aucucuugga uuagacugug aaccaagaac aggcuuagac     1200

uucucugauc uauauuaucu uacaaugaau aacaaacauu gguuaguaca uaaagagugg     1260

uuucaugaua uaccauuacc auggcacgca ggagcagaua cagggacgcc acauuggaac     1320

aauaaagaag cucuaguaga auuuaaagau gcacacgcaa aaagacaaac aguaguuguu     1380

uuagguucuc aagaaggagc cguacacaca gcacuagcug gagccuuaga agcagaaaug     1440

gacggggcua aagguagauu aucaucugga cacuuaaaau guagauuaaa aauggauaaa     1500

uuaagauuaa aaggaguaag uuauagccug uguacagccg cauuuacuuu uaccaaaaua     1560

ccagcagaaa cauuacaugg uacaguaacc guagaagugc aguaugcagg aacagauggg     1620

ccuuguaaag ucccagcuca aauggcagua gauaugcaaa cacuuacccc aguaggcaga     1680

cuuauaacug caaauccagu gauaacagaa ucaaccgaga acuccaaaau gaugcuagaa     1740

cuagauccac cauucggaga uaguuauaua guuaucggag ucggagaaaa gaaaauaacu     1800

caucauuggc auagaucagg uagcacgaua ggaaaagcau uugaagcaac agucagaggu     1860

gccaaaagaa uggcaguauu gggagauaca gcaugggacu uuggaagugu cggaggagca     1920

cuaaauagcu uaggaaaagg aauacaucaa auuuucggag cagcauuuaa aucuuuauuu     1980

ggaggaauga gcugguucuc acaaauacuu auaggaacau ugcugaugug guuaggacuc     2040

aauaccaaaa acggaagcau aucauuaaug ugucuagcac ugggaggugu cuuaauauuc     2100

cuaucgacag caguaucagc agauguggga uguugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  9134
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9134
gggagaaagc uuaccaugau caacgcaaga aaagaaaaaa aaagacgagg agcagacaca       60

aguguaggaa uaguaggauu acuuuugaca acugcaaugg cagcggaagu aacaagaaga      120

ggaagugcau auuauaugua uuuagaccgc aaugaugccg gugaagcgau aucauuccca      180

acuacuuuag gaaugaauaa auguuauaua caaauaaugg auuuaggaca uaugugugau      240

gcaacgauga gcuaugaaug ccccauguua gaugaaggag uagaaccaga cgauguagac      300

uguuggugua auacaacaag uacaugggua guauauggaa cgugucauca caaaaaagga      360

gaagcaagga gaaguaggag agcaguaacc uuaccauccc auagcacucg aaaauuacaa      420

acaagaagcc aaacaugguu agaaagucga gaauauacaa aacauuuaau aagagucgaa      480

aauuggauau uuagaaaucc aggauucgca uuagcagcag cugcaauagc auggcuauua      540

gguucaucaa caagucaaaa aguaauauau uuaguaauga uuuuacuaau agcaccagca      600

uauucaauaa gauguauugg aguaagcaac cgcgauuuug uagaaggaau gucaggagga      660

acauggguag acauaguacu agaacaugga ggguguguca caguaauggc acaagauaaa      720

ccaacgguag auauagaauu aguaacgacc accguuucua acauggcuga aguacgauca      780

uauugcuacg aagcaucaau aagcgacaug gcaucagauu cuagaugucc aacucaagga      840

gaagcguauu uggauaaaca auccgauaca caauacguau gcaagaggac ucuaguagau      900

agggguuggg gaaacggaug uggguuauuu ggaaaaggaa guuuaguuac augugcuaag      960

uuugcgugua guaaaaaaau gacaggaaag ucaauucaac cagaaaauuu ggaauacaga     1020

auaauguuaa gcguacaugg cucacaacac agcggaauga uagugaauga cacaggacau     1080

gaaacagaug aaaauagagc aaaaguagaa auaacaccaa auaguccaag agcagaagcg     1140

acacuaggag guuuugguuc auuaggucua gauugcgaac cucgaacagg uuuagacuuu     1200

ucagaucugu auuauuuaac aaugaacaau aaacauuggu uaguccauaa agaaugguuu     1260

cacgauauac cuuuaccgug gcacgccgga gcagacacag gaacacccca uuggaauaau     1320

aaagaagcac uaguagaguu uaaagacgcu caugcaaaaa gacaaacugu aguaguuuug     1380

gguagccaag aaggagccgu acauacagca uuagcaggag cgcuagaagc agagauggac     1440

ggcgcuaaag gaagauuauc cuccggacau uuaaaauguc gauuaaaaau ggauaaauua     1500

agacuaaaag gaguaucaua uaguuuaugc accgcagcuu uuacauuuac aaaaauaccu     1560

gcagaaacuc uacauggaac cguaacagua gaaguucaau augcaggcac agauggacca     1620

uguaaaguuc cugcacaaau ggcuguagau augcaaacac ucacacccgu uggaagauua     1680

auaacagcga acccaguaau aacugaaagc acagaaaaua guaaaaugau guuagaauua     1740

gacccaccau ucggagauag uuauauagua auagguguag gagaaaaaaa aauaacucac     1800

cauuggcaca gaucaggguc aacuauugga aaagcauuug aggcaacagu aagaggagcg     1860

aaacguaugg caguauuagg agacaccgcg ugggacuuug guagcguagg aggagcauua     1920

aacucguuag gaaaaggaau ccaucagaua uucggagcag ccuuuaaaag ucucuucgga     1980

ggaaugagcu gguucucaca aauuuuaaua ggaacauuac uaauguggcu aggauuaaau     2040

acaaaaaaug gcaguauauc auuaaugugc uuagcucuag gaggaguacu aauauucuua     2100

aguacugcag ucucagcuga ugugggaugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  9135
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9135
gggagaaagc uuaccaugaa cgcaagaaaa gaaaaaaaac gucguggugc cgauacaagu       60

guaggaauag uaggauuauu guuaacaaca gcaauggcag cagaaguaac acgaagagga      120

ucagcauauu acauguauuu ggauagaaau gaugcgggag aagcaauauc auuuccaacu      180

acacuuggaa ugaauaaaug uuauauucaa auuauggauu uaggacauau gugcgacgcu      240

acaaugucau acgaaugccc gauguuagac gaaggggugg aaccagauga cgucgauugu      300

ugguguaaua caacgucaac guggguagua uauggaacau gccaucacaa aaaaggcgaa      360

gcuagaagaa gucguagagc aguaacauua ccaagccaua guacaagaaa auuacaaaca      420

cgaucccaaa cuugguuaga aucaagggaa uacacuaaac auuugauaag aguagaaaau      480

uggauauuua gaaauccggg uuucgcccuu gcggcagcag caauagcaug guuacuagga      540

ucuucaacaa gucaaaaagu aauauauuua guaaugauac uuuuaauagc accagcuuau      600

agcauaagau gcauuggugu aaguaauagg gacuuuguag aagguauguc agguggaaca      660

uggguagaua uaguacuaga acauggaggu ugcguaacag uuauggcaca agauaaacca      720

acggucgaca uagaacuagu uacaacaaca guuagcaaua uggcagaagu aagaucauau      780

uguuacgaag caucuauaag cgacauggca agugauucua gauguccaac acaaggagag      840

gcauaccuug auaaacaaag ugauacucaa uauguaugca aaagaacauu aguagaucga      900

ggauggggaa acgggugugg ucuauuuggu aaaggaucau uaguaacgug ugcaaaauuu      960

gcauguucga aaaaaaugac aggaaaauca auacaaccag aaaacuuaga auauagaaua     1020

augcucagcg uacaugguuc acaacauuca ggaaugauug uaaaugauac aggucaugaa     1080

acagaugaaa accgagcaaa aguagaaauu acuccaaaua guccaagagc ugaagcaaca     1140

cuuggagguu uuggaagucu aggacuagac ugcgaaccaa gaacuggauu agauuuuucu     1200

gaucuauacu aucuaacaau gaacaauaag cauugguuag uacauaaaga gugguuucau     1260

gauauuccac uaccuuggca ugcaggugcu gauacaggaa ccccacauug gaauaauaaa     1320

gaagcuuugg uagaauuuaa agaugcacau gcaaaaagac aaacuguagu aguauuagga     1380

ucacaagaag gagcaguaca caccgcacua gcaggagcuu uagaagcuga aauggaugga     1440

gccaaaggaa gauuauccag uggacauuua aaaugcagau uaaagaugga caaacuacga     1500

cuaaaaggag uaagcuauuc auuauguaca gcagcauuua cauuuaccaa aauaccugcu     1560

gaaacucuac acggaacagu aacaguagaa guacaauaug cggguacaga uggaccaugu     1620

aaaguaccag cucaaauggc uguagauaug cagacuuuaa caccgguagg aagauugaua     1680

acugcaaauc cuguaauaac ugaaucaaca gaaaauagua aaaugauguu agaauuagau     1740

ccaccauucg gagauucaua cauaguaauu ggugucgggg aaaaaaaaau aacccaucau     1800

uggcauagau caggaaguac aauaggaaaa gcuuuugaag caacaguaag aggagcaaaa     1860

agaauggcgg ugcucggaga uacagcuugg gauuuuggca guguaggugg ugcacuaaau     1920

ucauuaggua aaggaauaca ucaaauuuuu ggagcugcgu uuaaaucacu auucggagga     1980

augucuuggu uuucucaaau auuaauuggg acuuuguuaa ugugguuagg ucuaaauaca     2040

aaaaaugggu caauaucacu aauguguuua gcacugggag gaguauuaau uuuuuuaagu     2100

acagcaguaa gcgcagacgu aggauguuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9136
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9136
gggagaaagc uuaccauggc cagaaaagaa aaaaaaagaa gaggagcuga uacaucagua       60

ggaauaguag gacuauuguu gacaacagca auggcagcag agguaacaag acgagguagc      120

gcauauuaca uguauuuaga uagaaacgau gcaggagaag cuauaucauu cccaacuaca      180

uuaggaauga acaaaugcua uauacaaauu auggauuuag gacauaugug ugaugcaaca      240

augaguuaug aaugcccuau guuagaugaa ggaguagaac ccgacgaugu ugacugcugg      300

ugcaauacga caaguacaug gguuguauau gguacauguc aucauaaaaa aggagaagca      360

agacgaagua gacgcgcagu cacauuaccg agccauagca cgcguaaacu acaaacacga      420

ucacaaacuu ggcuagagag uagagaauau acuaaacauu uaaucagagu cgaaaacugg      480

auauucagga aucccggauu ugcauuagca gcagcagcaa ucgcauggcu auuaggcuca      540

aguacuaguc aaaaaguaau auauuuagua augauauuac uaauugcccc agcauacagu      600

auaagaugua uagguguaag caaccgagac uucguagaag gaaugucugg aggaacaugg      660

guugacauug uacuagaaca cggagguugu gucacaguga uggcgcaaga uaaaccuaca      720

guugauauug aacuaguuac aacaacagua aguaacaugg cugaaguaag aucauacugu      780

uaugaagcau cuauaaguga uauggcuagu gacagucgau guccaacaca aggcgaagca      840

uacuuagaua aacaaucaga uacucaauau guauguaaaa gaacacuagu cgacagaggg      900

uggggaaacg gauguggacu auuuggaaaa ggaucgcuag ugacaugugc aaaauuugca      960

ugcucaaaaa aaaugacagg caaaucaaua caaccugaaa auuuagaaua ccggaucaug     1020

uuaucagucc augguucaca acauagcgga augauaguca augauacagg acaugaaacg     1080

gaugaaaaca gggcaaaagu agaaauaacu ccaaacaguc caagagcaga agccacauug     1140

ggaggcuuug gaagcuuagg auuagacugc gaaccgcgua caggcuuaga uuucuccgau     1200

cuuuauuauc uaacuaugaa uaauaaacau ugguugguac auaaagaaug guuucacgac     1260

aucccauuac cuuggcaugc aggagcagau acaggaacac cacauuggaa caauaaggaa     1320

gcauuaguag aauucaaaga cgcacaugca aaaagacaaa caguaguagu acuaggauca     1380

caagaaggag caguccauac ugcucucgca ggagcguuag aagcagaaau ggacggagca     1440

aaagggcguu ugagcucagg acauuuaaaa ugcagauuaa aaauggauaa auuacgauua     1500

aaaggaguau cuuacucgcu auguaccgca gcuuucacau ucacaaaaau accagcagaa     1560

accuuacaug ggacaguaac gguugaagug caauaugcag ggacagaugg cccauguaaa     1620

guacccgcuc aaauggcagu ugacaugcaa acucuaacac caguaggaag auuaauuaca     1680

gcaaacccag uaauuacaga aaguacugaa aacaguaaaa ugauguugga acuagaucca     1740

ccauuuggag acagcuauau aguuaucggc guaggugaaa aaaaaauaac acaucauugg     1800

cauagaagug guaguacgau aggaaaagcu uucgaagcua cgguaagagg agcaaaaaga     1860

auggcagucu ugggagauac cgcaugggau uuugguagug uugguggugc auuaaauuca     1920

uuagguaaag gaauacacca aauauuugga gcagcauuca aaucuuuauu cggagguaug     1980

aguugguuuu cacaaauauu gauaggaaca uuacuaaugu ggcuaggcuu aaauacaaaa     2040

aaugguucca uaaguuuaau guguuuagca uuagggggug uguuaauauu uuuaucuacu     2100

gcaguuagug cugauguagg cuguugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  9137
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9137
gggagaaagc uuaccaugag aaaagaaaag aaaagaagag gugcagacac uucaguagga       60

auugugggau ugcuacuuac aacugcaaug gcagcagaag uaacacgacg uggaucugca      120

uacuauaugu acuuagacag gaaugacgcu ggugaagcaa uaaguuuucc aacaacauua      180

ggcaugaaca aaugcuacau acaaauaaug gaccuaggac auauguguga cgcaaccaug      240

aguuacgaau guccaauguu agaugaagga guagagcccg augauguaga uugcuggugu      300

aauacaacaa gcacaugggu aguguaugga acaugucacc auaagaaagg ugaagcaaga      360

agaucaagga gagcaguaac ucuuccauca cauucaacgc gaaaauuaca aacaagguca      420

caaacauggu uagaaaguag agaauauacg aaacaccuca uaagaguaga aaauuggaua      480

uucagaaauc ccggcuuugc acuagcagca gcagcaauag caugguuacu gggaaguagu      540

acaucacaga aaguaauaua uuugguaaug auauuacuua uagccccagc auauucaauu      600

agauguauag gaguuucgaa uagagauuuc guggaaggca ugaguggagg aacgugggua      660

gacauaguuu uagaacaugg aggauguguc acagugaugg cacaagauaa accgacagua      720

gauauagaau uagugacaac uacaguaagu aauauggcag aaguuagaag uuauuguuau      780

gaagcaucca uaagcgauau ggcuucagau ucaagauguc cgacucaagg agaagcauau      840

uuagauaaac aaagugacac acaauacgua uguaaaagga cucuaguaga uagaggaugg      900

gguaauggau guggguuauu ugguaaagga ucuuuaguca caugugcaaa auucgcaugc      960

aguaagaaaa ugacaggcaa aaguauccag ccagaaaauu uggaauauag gauaaugcua     1020

ucaguacacg guucucaaca uuccggaaug auugucaaug auacagguca ugaaacagau     1080

gaaaacaggg cgaaagugga gauuacacca aacucaccaa gagcggaagc aacacuaggu     1140

ggauuugguu cauugggauu agauugcgaa ccaagaacag gauuagauuu uucagaucug     1200

uacuauuuaa cuaugaacaa caaacauugg uuaguacaua aagaaugguu ucaugauaua     1260

ccauuaccau ggcaugcggg agcagauaca ggaacgccac acuggaacaa caaagaagca     1320

cucguagaau uuaaagacgc acaugcgaaa cgacaaacag uaguaguauu aggaagccag     1380

gaaggagcag uacauacagc acuagcaggc gcauuagaag cagaaaugga uggagcaaaa     1440

gguagauuaa guaguggaca uuuaaaaugu agguuaaaaa uggauaaacu aagauuaaaa     1500

ggaguaaguu auaguuuaug cacugccgca uucacauuua ccaaaauacc ugcagagaca     1560

uuacauggca caguaacagu ugaaguacaa uacgcaggca cagaugggcc augcaaagug     1620

cccgcucaaa uggcaguaga uaugcaaacc uuaacgccag uuggcagauu aauaacugca     1680

aacccaguua uaacugaauc gacagaaaac agcaaaauga uguuagaacu agauccgccu     1740

uuuggagauu cauauauagu aauuggagua ggagaaaaga aaauaacaca ucacuggcau     1800

agaaguggaa gcacaauagg aaaagcauuu gaagcgacag uaaggggagc gaaaagaaug     1860

gccguauuag gagauacagc augggacuuc gguaguguag gaggagcuuu aaauagucua     1920

ggaaaaggaa uacaucaaau auuuggagca gcuuuuaaau cacuauuugg ugguauguca     1980

ugguuuucac aaauauuaau aggaacauua cuaauguggc uaggccuaaa uaccaaaaau     2040

ggaaguauaa guuuaaugug uuuagcauua ggugguguau uaauauuucu aaguaccgca     2100

guaucagcag auguuggaug uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9138
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9138
gggagaaagc uuaccaugaa agaaaagaaa agaagaggug cagauacauc cguaggcaua       60

guagguuugu uauuaacaac agcaauggcu gccgaaguga cuagacgugg aagugcauac      120

uacauguauu uagauagaaa ugaugcugga gaagcuauuu cauuuccuac cacauuagga      180

augaauaaau guuacauaca aauaauggau cuaggucaca ugugcgaugc cacaaugagc      240

uacgaauguc caauguuaga ugagggagua gaaccagacg auguugauug uugguguaau      300

acaacaucaa cguggguagu uuaugguaca ugccaucaua agaaaggaga agcuagaaga      360

ucaagacgag caguaacauu accaagucac ucaacuagaa aacuacaaac aagaagccaa      420

acauggcuag aaagcagaga auauacuaaa caccuuauua gaguagaaaa uuggauauuu      480

aggaacccag gauuugcuuu agcggcugcu gcaauugcau ggcugcuugg aucaaguaca      540

ucucagaaag ucauauaucu gguaaugauc uuauuaauag ccccugcaua caguaucagg      600

ugcauaggag uaucgaaucg ugauuucgua gaaggaauga gcgggggaac auggguugac      660

auaguauuag agcauggagg auguguuaca guaauggcac aagauaaacc aacugucgau      720

auagaauuag uaaccaccac aguaagcaac auggcagaag uaagaaguua uuguuaugaa      780

gcaucaauaa gcgauauggc aucugacuca cgauguccaa cacaagggga agcauaucuc      840

gauaaacagu cagauacuca auauguuugu aaaagaacuc uaguagaucg aggcugggga      900

aacggaugcg gucucuucgg aaaaggauca uuaguaacau gugcaaaguu cgcauguagu      960

aagaaaauga caggaaaauc cauacaacca gaaaauuuag aauauagaau uauguuaagc     1020

guacaugguu cucaacacuc aggaaugaua guaaaugaua caggccauga aacagacgaa     1080

aacagagcca aaguagagau aacaccaaac aguccacgag cugaagcuac auuaggaggg     1140

uucggaucau uaggacugga uugugaaccc agaacugguc uagacuuuuc agaccuuuac     1200

uauuuaacaa ugaauaauaa acauuggcua guacacaaag aaugguuuca cgacauuccg     1260

uuaccauggc augcgggugc agauacaggc acaccacauu ggaauaauaa agaagcucua     1320

guagaguuua aagaugcaca ugcaaaacga caaacagucg uaguacuagg uagucaagaa     1380

ggagcaguac auacugcauu agcuggagca uuagaagcag aaauggaugg agcaaaagga     1440

aggcuaucau ccggucaucu aaaauguaga cuaaaaaugg auaaacugag auuaaaggga     1500

gucagcuauu cauuauguac agcagcauuc acauucacca aaaucccagc agagacuuua     1560

cauggaacag uaacagucga aguacaauau gcagguacag auggaccaug uaaaguucca     1620

gcacaaaugg cuguagauau gcaaacguua acacccguug guagacuaau aacagcuaac     1680

ccaguuauua cagaaagcac agaaaauuca aaaaugaugu uagagcuaga cccaccauuc     1740

ggugauagcu auauugucau aggagucgga gaaaagaaaa uaacacacca uuggcacaga     1800

ucaggaucaa caauaggaaa agccuuugaa gcuacaguaa gaggagcaaa acgaauggcc     1860

guauuaggug auacagcaug ggauuuugga ucaguuggag gagcacuuaa cucucuaggu     1920

aaaggaauac aucaaauauu uggagcagcu uucaaaucuc uauuuggugg uaugaguugg     1980

uuuagucaaa uauuaauugg uacauuacua augugguuag gucuaaauac uaaaaauggu     2040

ucaauaaguu uaauguguuu agcauuagga ggcguuuuaa uauuuuuaag uacagcagua     2100

agcgcugacg uuggcuguug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9139
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9139
gggagaaagc uuaccaugga aaagaaaaga agaggagcag auacaagugu aggaauuguu       60

ggacuauuau uaacuacagc cauggcagca gaaguaacga gaagggguag ugcuuauuac      120

auguaucuag acaggaauga ugcaggggaa gcaauaucuu ucccaacuac auuaggcaug      180

aauaaauguu auauucaaau aauggaucua ggacauaugu gugaugcaac aaugucauau      240

gaaugcccaa uguuagauga ggguguggaa cccgaugaug uagauugcug guguaauaca      300

acaagcacau ggguuguaua uggaaccugc caucauaaga aaggagaagc aagaagaucu      360

aggagagcag ugacguuacc aagucauucc acaagaaaau uacaaacaag aucgcagaca      420

ugguuagaaa gcagagaaua cacaaaacac uuaauaagag uagaaaauug gauuuucaga      480

aauccagguu uugcauuagc agccgcagca auagcauggc uccuaggauc aaguacaucu      540

caaaaaguua uauauuuagu caugauacua cuaauugcuc cugcauauag uauaagaugc      600

auagguguua guaauagaga uuuuguagaa gggaugucag gagguacaug gguagauaua      660

guauuagaac auggaggaug uguaacugua auggcacaag acaaaccuac aguagauaua      720

gaacucguaa cuacaaccgu aaguaauaug gcagagguaa gaucauauug uuacgaagca      780

ucaauaucag auauggcauc agauaguaga ugcccuacgc aaggagaagc auaucuagau      840

aaacaaagcg auacucaaua uguauguaaa cgaacuuuag uagauagagg guggggaaau      900

ggauguggac uauuugguaa aggaucuuua guaacuugug caaaauuugc uuguucaaag      960

aaaaugacug gaaaaucaau ucaaccagaa aauuuagaau aucgaauaau guuaucagua     1020

cauggaucgc aacacagcgg aaugauagua aacgauaccg gacacgaaac ugacgaaaac     1080

agagcaaaag uagagauaac acccaauagu ccaagagcag aagcuacacu aggaggauuu     1140

ggcagccuug gacucgacug ugagccaaga acgggauuag acuuuagcga ucuauauuac     1200

uuaacaauga auaacaaaca uugguuaguc cauaaagaau gguuucauga cauuccacua     1260

ccauggcacg cuggugcaga uacuggaaca ccgcacugga auaauaaaga agcccuaguu     1320

gaauuuaaag augcacaugc aaaaagacaa acagucguag uauuagguuc ucaggaagga     1380

gcaguucaua cagcauuagc cggugcauua gaagcagaaa uggauggagc aaaaggaaga     1440

uuaaguucug gacaucugaa augucgacua aaaauggaca aacuaagauu aaaaggagua     1500

ucauacucau uauguacagc agcauuuaca uucacuaaaa uaccagcaga aacauugcac     1560

gguacaguaa caguagaagu gcaauaugcu ggaacugaug gcccguguaa aguaccggca     1620

caaauggcug uagauaugca gacauuaaca ccaguaggaa gauuaauaac agcaaauccu     1680

guaauaacag aaagcacaga aaacaguaag augauguuag aauuagaccc acccuucggg     1740

gacagcuaua uaguuauagg aguuggagaa aagaaaauaa cacaucacug gcauagguca     1800

ggaucaacaa uagggaaagc auucgaagcg acuguuagag gagcaaaaag aauggcggua     1860

uugggagaua cugcauggga uuucggauca guaggaggag cauugaauag ucuaggcaaa     1920

gguauacacc aaauauucgg ggcagcauuu aaauccuuau uugguggaau gaguugguuu     1980

agucaaauuu uaauaggaac uuuacugaug ugguuaggac uaaauacuaa aaauggaucc     2040

aucucauuaa ugugucucgc gcuaggagga guacuaauuu ucuuaucuac agcaguauca     2100

gcggacguag gaugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9140
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9140
gggagaaagc uuaccaugaa aaaaagaagg ggagcugaua caagcgucgg aauaguagga       60

cuauuauuaa caacagcaau ggcagcagaa guaacacgaa gaggcagcgc uuauuauaug      120

uauuuagaua gaaaugaugc aggugaagcc auaucauuuc caacaacucu aggaaugaau      180

aaauguuaca uacaaauaau ggauuuagga cauaugugug augcaacaau gaguuaugaa      240

ugcccaaugc uggaugaagg uguugaaccu gaugauguag auuguuggug uaauacaaca      300

ucaacauggg uaguauacgg aacaugccau cacaagaaag gagaagcaag aagaucaaga      360

agagcaguaa cacuaccaag ucacucuaca agaaaauuac aaacaagaag ucaaacgugg      420

uuagaaucaa gagaauauac aaaacacuug auacgaguag aaaacuggau cuuuagaaau      480

ccaggauucg cccuugcagc cgcugcaauu gcaugguuac uagguaguag uacuagucaa      540

aaaguuauau accuugucau gauauuauua auagcuccag cauacucaau aagaugcaua      600

ggcguaagua auagagauuu ugucgaagga auguccggag gaacaugggu ugacauagua      660

uuagaacacg gaggaugugu aacaguaaug gcacaagaua aaccaacugu ugauauugag      720

uuaguuacaa cuacaguauc aaauauggca gagguacgaa guuauuguua cgaggcaagu      780

auaucugaua uggcaucuga uucuagaugu ccaacucaag gagaagcaua ucuugacaaa      840

caaucugaua cacaauaugu cuguaaaaga acauuaguag aucguggaug ggggaacgga      900

uguggauuau ucggaaaagg aucacuagua accugugcua aauuugcaug uuccaaaaaa      960

augacuggua aaucaaucca accagaaaau uuagaauaca ggauaauguu aucaguccac     1020

gguagucaac auagcgguau gauaguuaau gauacaggac augaaacaga ugaaaaucga     1080

gcaaaaguug aaauaacccc aaauucacca agagcagaag ccacacuagg gggcuuugga     1140

ucauugggau uagacugcga accucgaaca ggauuggacu ucucugauuu auauuaucua     1200

acgaugaaca auaaacacug guuaguacac aaagaauggu uucacgauau accucuacca     1260

uggcaugcag gugcagacac aggaacacca cauuggaaca auaaagaggc acuaguagaa     1320

uucaaagaug cgcacgccaa acgacaaaca gucguaguuu uagguucaca agaaggagcu     1380

guacauacag cuuuagcagg ggcguuagaa gcagaaaugg auggugcgaa aggacguuug     1440

ucaucugguc auuuaaaaug ucgauugaaa auggauaaac uacgacuaaa aggaguaagc     1500

uacucacuuu guacugcggc uuuuacauuu acaaaaauac cagcagaaac ccugcaugga     1560

acuguuacug uagaaguaca auaugcuggc acagacggac cuuguaaagu uccagcccaa     1620

auggcaguag auaugcaaac acuaacucca guaggaagac uuauaacagc aaauccagug     1680

auuacagaau caacagaaaa uaguaaaaug auguuagaac ucgauccacc auuuggagau     1740

agcuacauag ucauaggagu aggcgaaaaa aaaauaacac accauuggca uagaagcgga     1800

aguaccauag gaaaggccuu ugaagcgaca guaagaggag cuaaacgaau ggcaguacua     1860

ggggauacgg caugggacuu ugguagcgua ggaggagcau uaaacucucu cggaaaagga     1920

auacaccaaa ucuuuggagc ugcauuuaaa ucauuauuug guggcaugag uugguuuagc     1980

caaauccuaa uaggaacauu auuaaugugg uuagguuuaa acacaaaaaa uggauccauu     2040

ucauugaugu gucuggcacu uggaggagua uuaaucuuuc uuuccacugc aguaagcgca     2100

gauguaggau guugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  9141
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9141
gggagaaagc uuaccaugaa gcgaagagga gcagauacuu caguaggaau aguaggacua       60

uuauuaacaa cagcaauggc cgcagaaguc acaagaagag gaucagcaua cuauauguac      120

cuagauagaa acgaugcugg agaagcaaua ucguucccaa caacacuagg aaugaauaaa      180

uguuauauuc agauaaugga ucucggacau augugcgaug caacaauguc auaugaaugu      240

ccaauguuag augaaggagu cgaaccugau gauguagauu guugguguaa uacaacauca      300

acauggguag uauauggaac augucaucac aaaaagggcg aggcuagaag aucccguaga      360

gccgucacau uaccaucgca uaguacaaga aagcuacaaa cacguucuca gacauggcua      420

gaaaguagag aauauacaaa acauuuaauc cgagucgaaa acuggauauu ccgcaaucca      480

ggguuugcac uagcugccgc agcuauugca uggcugcuag guucaucaac gucacaaaaa      540

guuauauacc uagugaugau auuauuaauc gcaccagcau auaguauuag auguauaggu      600

guaaguaaua gagacuuugu agaagguaug agcggaggaa caugggucga uauaguauua      660

gaacauggcg gaugugugac cguaauggca caagauaaac ccacgguaga uauagaauug      720

gucacaacua cuguaucaaa uauggcagaa guaagaucau acugcuacga agcaucaauc      780

ucagauaugg ccucagauuc cagauguccg acccaaggag aagcauaucu agacaaacaa      840

ucggauacac aauauguaug uaaacgaacg cuaguugaua gaggaugggg aaauggaugu      900

ggauuauuug gaaaaggaag uuuagucaca ugugcaaaau ucgcaugcag caagaaaaug      960

accggaaaau ccauacaacc agaaaaucua gaauauagaa uaaugcuauc aguacacgga     1020

agccagcaua guggaaugau uguaaaugau accggacaug aaacugacga aaauagagca     1080

aaaguagaaa uaacaccaaa uaguccaaga gcagaagcaa cauuaggggg auucggauca     1140

cuagguuuag acugcgaacc acgaaccgga uuagauuuca gugacuuaua cuauuuaaca     1200

augaauaaua aacauugguu aguacacaaa gaaugguuuc augacauacc auuaccaugg     1260

caugcaggag cagacacagg aacaccacac uggaacaaua aagaagcauu aguagaauuu     1320

aaagaugcac augcaaaacg gcaaacagua guaguauuag gaagucaaga aggcgcugua     1380

cacacagcac uagcuggagc auuggaagca gaaauggacg gagcaaaagg aagguuauca     1440

aguggacacu uaaaauguag auuaaagaug gauaaacuaa gacuaaaagg aguaaguuau     1500

ucauuaugca cagcugcuuu uacauuuacu aaaaucccag cagaaacacu gcacgggaca     1560

guaacaguug aaguacaaua ugcagguacu gauggaccau guaaaguacc ugcucaaaug     1620

gcaguugaua ugcaaacauu aacaccagua gggagauuaa uuacugcaaa cccaguaaua     1680

acagaaucua cugaaaauuc aaaaaugaug uuagaauuag auccaccguu uggagauagu     1740

uauauaguaa uaggaguugg agagaaaaag auaacacauc auuggcacag aagcggauca     1800

acuauaggaa aagcauuuga agccacagua agaggagcaa aaagaauggc aguguuagga     1860

gauacagcau gggauuuugg aucaguggga ggugcacuua auucauuagg aaaagguauu     1920

caccaaauau ucggagcagc cuuuaaaagu cuuuucgggg guaugaguug guucagccaa     1980

auacuaauag gaacacuacu uaugugguua ggauuaaaua cuaaaaaugg uaguauaucu     2040

uuaauguguu uagcauuagg cggaguacug auauuuuuau caacagcggu uagcgcugac     2100

guggguugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9142
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9142
gggagaaagc uuaccaugag gcguggcgca gauacaucag uagggauugu aggauuauua       60

uuaacaacag caauggcagc agaaguuaca agaagaggaa gugcauacua cauguauuua      120

gauagaaacg augccggaga agcaauaucc uuuccaacaa cauuaggaau gaauaaaugu      180

uauauacaaa uaauggacuu aggacacaug ugugacgcaa cuaugucaua ugaaugccca      240

augcuagaug aaggagucga accugaugac guagauugcu gguguaauac aacaaguaca      300

uggguaguau auggaacaug ucaucauaag aaaggcgaag caagacgauc aaggagagca      360

guuacacugc ccucacacuc aacaagaaaa cuacaaacaa gaucacaaac uugguuagaa      420

ucuagagaau auacaaaaca ucuuauaaga gucgaaaauu ggauauuuag aaacccagga      480

uuugcauuag ccgcagcagc aauagcaugg uuauuaggga guucaacauc acagaaaguu      540

auauauuuag uaaugauauu auuaauugca ccagcauacu caauaagaug uauaggaguu      600

ucgaauaggg auuucguaga aggaaugucg ggagguaccu ggguagauau aguuuuagaa      660

cauggcgguu guguaacagu uauggcacaa gauaaaccua caguagauau agaacuugua      720

acaacaacag uuagcaacau ggcugaagua agaagcuauu guuacgaagc aucaauauca      780

gacauggcau ccgauucaag augcccgaca caaggagaag cauaucuaga uaaacaauca      840

gauacacaau auguaugcaa acgaacucua guagacagag gcuggggaaa cgguugcgga      900

cuauucggaa aagguagccu aguaaccugu gcuaaauucg cauguucaaa gaaaaugacu      960

ggaaaaucaa uucaaccuga aaacuuagaa uauagaauua uguuaagcgu gcauggaagc     1020

caacauucag guaugauugu caaugauacg ggacaugaaa cagaugaaaa ccgugcaaaa     1080

guagaaauaa caccaaauuc cccgagggca gaagcaacac uagggggauu uggaucacua     1140

ggcuuagauu gugaaccuag aacuggauua gauuuuagug aucuauauua ccuaacaaug     1200

aacaauaaac auugguuagu ucauaaagaa ugguuucaug auauuccauu accguggcac     1260

gccggugcag auacagguac gccacacugg aauaacaaag aagcauuagu agaauuuaaa     1320

gaugcacaug cuaaacguca aacugucgua guauuagguu cacaagaagg agcaguacau     1380

acagcauuag caggagcccu agaagcggaa auggauggag caaaaggacg ccuaucaagu     1440

ggacacuuaa aaugucgcuu aaaaauggac aagcuaagau uaaaaggagu caguuauucu     1500

cuaugcacag ccgcuuucac auucacgaaa aucccugcgg aaacacuuca cggaacagua     1560

acuguagaag uucaauacgc agguacagac ggcccgugua aaguaccagc ccaaauggcc     1620

guagauaugc aaacauuaac accaguagga agacuaauaa cugcaaaucc aguuauaaca     1680

gaaucgacag aaaacagcaa aaugauguua gaacuagacc caccauuugg agauucuuac     1740

aucguuaucg gaguaggaga aaagaaaauu acacaccacu ggcauagaag uggaucaaca     1800

auuggaaaag cauucgaagc aacgguaaga ggagccaaac gaauggcagu uuuaggagac     1860

acugcauggg acuucggaag cguaggaggu gcauuaaacu cguuagggaa aggcauucau     1920

cagauauuug gagcagcauu caaaucauua uucggaggua ugucaugguu uagucaaaua     1980

cuaauuggaa cuuuacuaau gugguugggu uuaaacacua aaaacgguag uauaucacuu     2040

auguguuuag ccuuaggcgg aguguuaaua uuuuuaucaa cagcaguaag cgcugaugua     2100

gguuguugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  9143
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9143
gggagaaagc uuaccaugag aggagcugau acaucaguag gaauuguagg uuuauuacua       60

acaacagcaa uggcagcaga aguuacaaga agaggaucag ccuauuauau guauuuagau      120

cgaaacgaug ccggagaagc aauaaguuuu ccaacaacuu uaggaaugaa uaaaugcuau      180

auacaaauca uggaucuugg acacaugugu gacgcaacaa uguccuacga auguccaaug      240

uuagacgaag gaguagaacc ugacgacguc gauuguuggu guaauaccac aucaacaugg      300

guaguuuacg guacaugcca ucauaagaaa ggagaagcaa ggcgaucaag gagagcagua      360

acauuacccu cacauaguac aagaaaauua cagacuagau cacaaacaug gcuugaaucg      420

agagaauaca cuaaacaccu aauaagagua gaaaacugga uuuuuagaaa uccaggauuu      480

gccuuagcag cagcagcgau agcauggcua cuugggucua guacaaguca aaaaguaauu      540

uacuuaguaa ugaucuuacu aauugcacca gcauacucaa uaagaugcau uggaguauca      600

aauagagacu uugucgaagg uaugagcggu ggaacuuggg uagacauugu cuuggagcac      660

gguggaugcg uaacuguaau ggcacaagac aaaccaacag uggacauaga auugguaaca      720

acaaccguaa guaauauggc agaaguuaga uccuauugcu acgaggcauc aauaagugac      780

auggcaagug auucaagaug uccaacacaa ggagaggcuu aucucgauaa acaaagugau      840

acccaauaug uauguaaaag aacauuaguu gacagagguu ggggaaaugg cuguggacua      900

uucgguaaag ggagcuuagu aacaugugca aaauuugcau guucaaagaa aaugacagga      960

aaaucuauac aaccagaaaa ucuagaauau aggauuaugu uaagcguuca uggaucgcaa     1020

cauaguggaa ugauugucaa ugacacagga caugaaacug acgaaaaccg agcuaaagug     1080

gaaauaacac caaauucacc acgagcagaa gcuacacucg gaggcuucgg aucacuagga     1140

uuggacugug aaccaagaac agguuuagau uucagcgauc uauauuauuu aacuaugaau     1200

aauaaacauu gguuagugca uaaagaaugg uuucaugaua uaccauuacc uuggcacgca     1260

ggagcagaua caggcacacc acacuggaau aauaaagaag cccuaguaga auuuaaagau     1320

gcacacgcua aaagacaaac aguaguugua cuaggaucac aagaaggugc uguacauaca     1380

gcacuugccg gggcauuaga agcagaaaug gauggagcua aaggaagauu aagcucaggu     1440

cacuuaaaau guagauuaaa aauggauaaa uuaaggcuaa aaggaguaag cuacucuuua     1500

uguaccgcag cuuucacauu uacaaaaaua ccagcagaaa cauuacaugg aacaguaacu     1560

guagaaguac aauaugcagg uacagauggu ccauguaaag uaccagcaca gauggcagua     1620

gauaugcaaa cauuaacacc aguagggaga uugauaacag cuaauccugu cauaacagaa     1680

ucaacugaaa auagcaaaau gaugcuagaa cuagauccac cauucgguga uucauauaua     1740

guaauaggag uuggagaaaa gaaaauaacg caucacuggc auagaagcgg uaguacaaua     1800

ggaaaagcau uugaggcaac aguaaggggc gcaaaaagaa uggcuguauu aggagauaca     1860

gcaugggauu ucggaagcgu cgguggagca cuaaauucac uaggaaaagg uauucaucaa     1920

auauucggag cagcuuuuaa aucuuuguuu ggaggaauga guugguuuuc ucaaauacua     1980

auuggaacau ugcugaugug guuaggacua aacacuaaaa acggaucaau uagcuuaaug     2040

ugucuagcuc ucggaggugu auugauauuu uuaucaacag cuguaucagc ggacguugga     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  9144
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9144
gggagaaagc uuaccauggg agcugauaca agugucggaa uagucggacu auuacuaacu       60

acagcuaugg cagcggaagu uaccagaaga ggcagcgcau auuauaugua uuuagauaga      120

aaugaugcag gagaagcaau auccuuuccu accacucuag gaaugaauaa auguuauaua      180

caaauaaugg accuaggaca caugugcgau gccacuauga guuacgaaug cccuaugcuu      240

gaugaaggag ucgaaccuga ugauguagau ugcuggugua auacaacaag uacaugggua      300

guauacggaa caugccauca uaagaaagga gaggccagaa gaucaaggag agcaguaaca      360

cuacccucuc auucaacaag aaaauuacaa acaagaagcc aaacaugguu ggaaaguaga      420

gaauauacaa aacaucuaau ucgaguagaa aauuggauau uuaggaaccc aggauuugca      480

cuagcagcag cagcaauugc uugguuacua ggaucaucaa caucacaaaa aguaauauac      540

cuaguaauga uacuauuaau agcaccagca uauaguauaa gauguauagg aguaucuaac      600

cgagauuuug uugaaggaau gagcggggga acauggguag auauaguguu agaacaugga      660

ggauguguua ccguaauggc acaagauaaa cccacagugg acauagagcu aguuacaacu      720

accgucucaa auauggcaga gguacgaagc uauugcuaug aagcgagcau aagugauaug      780

gcaagugaua gccgaugucc uacccaagga gaagcauauc uagauaaaca aucagacacu      840

caauauguau gcaaacgaac auuaguugac agaggauggg ggaauggaug uggacuauuu      900

gggaaaggau cucuaguaac augugcaaaa uuugcuugua gcaagaaaau gacagguaaa      960

ucaauacaac cagaaaauuu agaguaucga auuaugcuaa gugugcaugg aucacaacau     1020

ucggguauga uaguaaauga cacuggacau gaaacugacg aaaauagagc gaaagucgaa     1080

auaacaccaa auuccccaag agcggaagcu acucuaggag gcuucggaag ucuggguuua     1140

gauugugaac cuagaaccgg acuagauuuc ucugauuuau auuauuuaac aaugaauaau     1200

aaacauuggu uaguacauaa agagugguuu caugauauuc cacuaccaug gcaugcuggu     1260

gcggauacag gaacuccaca uuggaacaau aaagaagcau uaguugaauu uaaagacgca     1320

caugcuaaac gacaaacagu cguaguacua ggaagucaag aaggagcugu ucacacagcu     1380

cuagcaggag cauuagaagc agaaauggau ggagcaaaag gaagauuauc aaguggacau     1440

cuaaaaugua gauuaaaaau ggacaaacuu agauuaaaag gaguaucuua uucucuaugu     1500

acagcugccu uuacauuuac aaaaauaccu gcugagacau uacacggaac aguuacaguu     1560

gaaguucaau augcaggcac ugauggacca uguaaggucc cagcucaaau ggcaguagau     1620

augcaaacau uaacaccagu uggaagauua auaaccgcaa auccuguaau aacagaaagc     1680

acagaaaauu caaaaaugau guuagagcua gauccaccau uuggagacuc auacauagua     1740

auaggaguag gugaaaagaa aauuacacau cauuggcaua gaucaggaag uacaauaggu     1800

aaagcauuug aagcaacagu aagaggagca aaaagaaugg caguccuugg cgauacugca     1860

ugggauuuug gaucaguugg aggagcacua aacucucuag gcaagggaau acaucaaaua     1920

uuuggagcag cuuucaaaag uuuauuugga ggaaugucau gguuuucuca aauacuaaua     1980

ggaacauuac uuaugugguu aggacuaaau acuaaaaacg gcaguauauc ucuaaugugu     2040

uuagcauuag gagguguuuu aauauuucuu ucuacagccg uaucggcaga uguuggaugu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  9145
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9145
gggagaaagc uuaccauggc ugacacauca guuggaauag uaggacuauu auuaacaacc       60

gcaauggcag cagaaguaac cagaagagga ucagccuauu auauguaucu cgauaggaac      120

gacgcaggcg aagcaauauc auuuccuaca acacuaggua ugaauaaaug uuauauucaa      180

auaauggauc ugggacauau gugcgacgca accauguccu augaaugucc uauguuagau      240

gaagguguag agccagauga uguugacugu ugguguaaua caacuaguac uuggguagua      300

uacggaacau gucaucauaa aaaaggagag gcccgaagau caaggagagc cguaaccuua      360

ccaagucauu caacaagaaa auuacaaacu aggagucaga cuuggcuaga aagucgagaa      420

uauaccaaac auuuaauacg aguagaaaau uggauuuuuc gaaauccagg auuugcacuu      480

gcugcugcug cgauugccug guuacuugga agcucaacaa gucaaaaagu aauuuauuua      540

guuaugauau uauuaauagc accagcuuac agcauuagau guauaggagu gucaaauaga      600

gacuuuguag agggcaugag uggagguacg uggguagaua uaguauuaga acauggagga      660

ugcgugacag uaauggcaca agacaaacca acgguagaua uagaauuagu aacaacuacc      720

guaucgaaua uggcagaagu acgaucauau ugcuaugaag caucaauaag ugauauggcu      780

uccgacagca gaugcccuac ucaaggagaa gcauacuuag auaagcaaag ugauacccaa      840

uauguuugca aacgcacacu aguagauaga ggauggggua auggaugugg uuuauuuggc      900

aaaggaaguc ugguaacaug ugcuaaauuu gcaugcucaa aaaaaaugac aggaaaauca      960

auacaacccg aaaauuuaga auauagaaua auguuaagug ugcaugguuc acaacauagc     1020

ggaaugauug uaaaugauac uggacaugaa acagaugaaa auagagcaaa aguagaaaua     1080

acaccaaaca guccaagggc ggaagccaca cuagguggau uugguucauu gggacuagac     1140

ugugaaccgc gaacuggcuu agauuuuagc gauuuauauu auuuaacaau gaauaacaaa     1200

cauugguuag uacacaaaga augguuccau gacauuccac uuccauggca cgcuggcgca     1260

gauacuggaa caccucacug gaauaacaaa gaagcacuag uggaauucaa ggacgcacac     1320

gcaaaaagac aaacaguagu aguucuagga ucacaagaag gagcaguaca uacagcuuua     1380

gcgggagcau uagaagcaga aauggaugga gcaaaaggaa gacuaucaag cggacaucua     1440

aaauguaggu uaaaaaugga caaacuaaga uuaaaaggag ugucauauag uuuauguaca     1500

gcagcauuca cuuuuacaaa aauuccagcu gaaacauuac augguacagu aacaguugaa     1560

guucaauaug cuggaacaga uggaccaugu aaaguaccag cacaaauggc aguagacaug     1620

caaacacuaa cacccguugg ccggcuaauc acagcaaauc caguaauuac agaaucaacu     1680

gaaaauucaa aaaugauguu agaauuagau ccacccuuug gagauaguua cauaguaaua     1740

ggcguaggag aaaaaaaaau uacacaucau uggcauagau cugggucaac aauuggaaaa     1800

gcguuugaag caacaguaag gggcgcuaaa cguauggcag uucuaggaga uacggcuugg     1860

gauuucggcu caguaggagg agcacuaaau ucauuaggaa aaggaauaca ucaaauuuuu     1920

ggagcagcau ucaaaucauu auuuggagga augagcuggu ucucacaaau uuuaauaggc     1980

acauugcuaa uguggcuagg auuaaauacc aaaaaugguu caauauccuu aauguguuua     2040

gcauuaggag gaguuuuaau auuuuuaagu acagcaguau cagcagacgu uggauguuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  9146
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9146
gggagaaagc uuaccaugga uacaucaguu gguauaguag gacuauuguu aacaacagca       60

auggcggcag aaguaacaag aagaggauca gcguacuaca uguaucuaga uagaaacgau      120

gcaggugaag caauaucauu cccaacgacc cuaggcauga acaaauguua uauacaaauc      180

auggaucuag gucauaugug ugaugcaaca augucuuaug aaugucccau guuagaugaa      240

ggcguagagc cugaugacgu agacuguugg uguaauacaa caaguacaug gguaguuuau      300

ggaacauguc aucauaaaaa aggagaagca agaagaagca gacgagcugu gacacuaccu      360

ucacacagua caagaaaauu acaaacaaga ucacaaaccu gguuggaaag cagagaauau      420

acaaaacacu uaauacgagu agagaacugg auuuuuagaa auccagguuu cgcauuagcu      480

gcagcagcaa uagcauggcu auuagguagu ucaacaucuc aaaaaguaau auauuuggua      540

augauauuac uaauagcucc agcuuauagu aucagaugua ucggcguauc aaauagagac      600

uuuguugaag guaugucagg aggaacaugg gucgauauag uucuugaaca ugguggaugu      660

guaacaguaa uggcacaaga caaaccuaca guagauauag aauuaguaac cacaacagua      720

ucaaacaugg cugaaguaag aucuuauugu uaugaagcaa gcauaucaga uauggcaagc      780

gacagcagau gcccaacaca aggagaagcu uaccuagaca aacaaaguga uacucaguac      840

guauguaaac ggacacuugu ggauagaggu uggggaaacg gauguggauu auuuggaaaa      900

ggcaguuuag uaacaugcgc aaaauuugcc uguucaaaaa aaaugaccgg aaaaucaaua      960

cagccagaaa aucuagaaua ccguauaaug uuaaguguac augguaguca acauaguggg     1020

augaucguaa augauacagg acaugaaaca gacgaaaaca gagcgaaagu agaaauuaca     1080

ccuaauucac caagagcaga agcaacauua gguggauuug gcucacuugg auuagauugc     1140

gaaccaagaa cuggacuaga uuuuucagau uuauacuauu uaacaaugaa uaauaaacau     1200

ugguuaguuc acaaagaaug guuucaugau auaccucuac cuuggcacgc gggagcagac     1260

acaggaacac cucacuggaa uaauaaagaa gcuuuaguug aauuuaaaga ugcucacgca     1320

aaacgacaaa cuguaguugu cuuagguucu caagaaggag caguacauac ugcauuagca     1380

ggcgcucuag aggcagagau ggauggagca aaagguagau uaucgagugg acauuugaaa     1440

ugcagauuaa aaauggacaa auuaagguua aaaggaguaa guuacucauu auguacagca     1500

gcauuuacau uuacaaaaau accagcagaa acauuacacg gaacuguaac cguugaagua     1560

caauaugcag guacagaugg accaugcaaa guaccagcac aaauggccgu agacaugcag     1620

acauugacuc caguaggaag auuaauaaca gcaaauccag uaauaacuga aagcacggaa     1680

aauaguaaaa ugaugcucga auuagaucca ccauuuggag auaguuacau cguaaucgga     1740

guuggcgaaa aaaaaauuac acaccacugg cauagaucug guucaacaau aggaaaagca     1800

uucgaagcaa caguacgcgg agccaaacga auggcaguuu uaggagauac agccugggau     1860

uuuggcagug uaggaggcgc ucucaauagu cuuggaaaag gaauacauca aaucuuugga     1920

gcagccuuua agagucuguu cggaggaaug aguugguucu cacaaauacu aauagguacg     1980

uuauuaaugu ggcuaggauu aaacacaaag aaugguucaa uaucuuugau gugucuagcu     2040

uuaggaggcg uacucaucuu uuugucaaca gcaguaucag cugauguagg augcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  9147
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9147
gggagaaagc uuaccaugac aaguguagga auuguaggau uacuacuaac aacagcuaug       60

gcagcagaag uaacaagaag gggcucagca uauuauaugu auuuagauag aaacgaugcu      120

ggggaagcaa uaagcuuccc uacaacauua ggaaugaaca aauguuacau acaaauuaug      180

gacuuagggc auauguguga ugccacaaug ucuuaugaau gcccaauguu agaugaagga      240

guagagccug acgauguaga cuguuggugu aauacaacau caacuugggu gguauauggc      300

acaugucauc auaaaaaagg cgaagcacga agauccagga gagcuguaac acuaccauca      360

cauaguacaa gaaaauuaca aacaaggucu caaacauggu uagaaucucg cgaauauacc      420

aaacaccuua uaagaguaga aaauuggaua uucagaaauc caggcuucgc acuagcagcu      480

gcagcuauag caugguuauu aggaucgucc acaucacaaa aagucauaua uuuaguaaug      540

auacuauuaa ucgcaccagc auauucaaua agguguauag gagugucaaa cagagauuuu      600

guugaaggaa uguccggagg aacaugggug gacauaguuc uugaacacgg aggauguguu      660

acaguuaugg cucaagauaa accaaccguu gacauagaau uaguaacaac aacuguaagu      720

aauauggcag aaguaagaag uuacuguuau gaagcaagca uaucagauau ggcaucagac      780

ucaagaugcc caacacaagg ugaagcauau uuggauaaac aaucagauac acaauaugua      840

uguaaaagaa cacuaguaga uagagguugg gggaacggau guggauuauu uggaaaaggc      900

agcuuaguaa caugugcaaa auuugcaugc agcaaaaaaa ugacaggaaa aucuauacaa      960

ccagaaaauc uagaauacag aauaauguug ucaguacaug ggucacaaca uucaggaaug     1020

auaguaaacg auaccgggca cgaaacagac gagaaccgag cgaaaguuga aauaacacca     1080

aacagcccaa gagcugaagc aacauuaggg ggauucggcu cauuaggacu agauugcgag     1140

ccaagaacag gauuagacuu uucagaucug uauuauuuaa caaugaacaa uaaacauugg     1200

uuaguucaua aagaaugguu ucacgauauu ccauuaccau ggcaugcggg agcagauacc     1260

ggaacaccac auuggaauaa caaagaagcu uuaguagaau ucaaagacgc acaugcaaaa     1320

agacaaacag uuguaguauu agguagccag gaaggggccg uacauacagc acuugcagga     1380

gcauuagagg cagaaaugga uggugcuaaa ggcagacuaa guaguggaca ucuaaaaugu     1440

aggcuaaaaa uggauaaauu gagacucaaa ggagucucau auagucuaug uaccgcagca     1500

uucacauuua caaaaauacc ugcagaaaca cuucauggaa caguaacugu ugaaguacaa     1560

uacgcaggga cugauggacc auguaaaguc ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccug uugguagguu aauaacagca aacccuguga uaacugaauc uacagaaaac     1680

agcaaaauga uguuagaguu agacccacca uuuggagaua guuauauagu cauaggagua     1740

ggcgaaaaaa aaauaacuca ucauuggcac agaucaggca guacgauagg uaaagcauuu     1800

gaggcaacag uacgcggagc gaaacgaaug gcaguacuag gagauacagc augggauuuc     1860

ggcucaguug gaggagcauu aaauucacuu ggaaaaggca uacaccaaau auuuggugca     1920

gcauucaaaa gccuauuugg aggaauguca ugguuuaguc aaauauuaau aggaacauug     1980

cuaauguggc uuggauuaaa uacaaaaaau ggauccauau cacuaaugug ccuagcauua     2040

ggaggaguau uaauauuccu aucuacggca gugagcgccg auguaggaug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9148
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9148
gggagaaagc uuaccaugac uagcguagga auagucggac uucuauugac aaccgcuaug       60

gcagcagcag uuacaagaag aggcuccgca uauuacaugu accuagacag aaaugaugcc      120

ggagaagcca uaagcuuucc aacaacacua ggaaugaaua aauguuacau ucaaaucaug      180

gaucucggac auauguguga ugcaacaaug aguuaugaau guccaauguu agacgaagga      240

guagaaccug augaugucga uugcuggugc aauacaacau caacaugggu cguauauggc      300

acuugccacc auaaaaaggg agaagcuagg cguucacgaa gagcaguaac acuaccaucc      360

cauucaacua gaaaauuaca aacccgaagu caaacuuggu uagaauccag agaauauaca      420

aaacacuuga uaagaguaga aaauuggaua uuuagaaauc cagguuucgc cuuagcagcu      480

gcagcaauug caugguuacu aggaagcuca acaucacaaa aaguuauaua cuuaguaaug      540

auacuauuaa uagcuccagc uuacucaaua agauguauag gaguuucaaa uagggauuuc      600

guagaaggga ugucaggagg uacauggguc gacauaguau uagaacaugg aggaugcgua      660

acaguuaugg cucaagauaa accaacgguc gauaucgaac uuguuacaac uacaguaucu      720

aauauggcag aaguaagaag uuauuguuau gaggcaucua uaagugauau ggcuagugau      780

aguagauguc caacacaagg ugaagcauac uuagauaaac aaucugacac acaauauguu      840

uguaaaagaa cacuaguaga ccgaggaugg ggcaacgggu guggauuauu uggaaaagga      900

aguuuaguaa caugcgcaaa auuugcaugu ucaaaaaaaa ugacaggaaa aucgauucaa      960

ccugaaaauc uagaauauag aaucaugcuu ucaguucacg gaucacagca uaguggaaug     1020

auaguaaaug auacagguca cgaaacggac gaaaauagag ccaaaguaga aauaacuccg     1080

aauucaccaa gagcggaagc cacucuuggc ggauuuggau cauuaggauu agauugugaa     1140

ccaaggacag gacuagauuu cucagaucua uauuaccuua caaugaauaa caaacauugg     1200

uuaguacaca aagaaugguu ucaugauaua ccacuaccuu ggcacgcagg agcugauacu     1260

gguacaccac acuggaacaa caaagaagcg uuaguagaau uuaaagaugc acaugcaaaa     1320

agacaaaccg uaguaguacu cggaagccaa gaaggagcag uacauacagc ucuagcugga     1380

gcauuagaag cagaaaugga cggagcaaaa ggaagauuau cuaguggcca ccuuaaaugu     1440

agauuaaaaa uggauaaguu aagacucaaa gguguaucau acucauuaug uacugcugca     1500

uuuacauuca cgaaaauacc ugcagaaaca cuacauggaa cuguaacugu ugaaguccaa     1560

uaugcaggua cagauggacc auguaaagua ccagcacaaa uggcugucga uaugcaaacg     1620

uuaacuccug uaggacgauu aauaacagca aauccaguua uaacggaauc cacagaaaac     1680

ucaaaaauga uguuagaauu agauccacca uuuggagauu cauauauagu uaucgguguu     1740

ggagaaaaaa aaauaacuca ucauuggcau agaagugguu caaccauagg aaaagcauuu     1800

gaggcaacug uaagaggagc aaaaagaaug gcugugcugg gagauacagc uugggauuuu     1860

ggaagugugg gcggagcauu aaauagcuua gguaaaggua uacaucaaau auuuggagca     1920

gcauuuaaau cacuauuugg uggaauguca ugguuuaguc aaauacugau agguacucuu     1980

uugauguggu uaggauuaaa uacaaaaaau ggaagcauaa guuuaaugug uuuagcauua     2040

ggaggaguau uaauauuucu aagcacagca guaucagcug acguaggaug uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9149
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9149
gggagaaagc uuaccaugag aggagcagau accaguguag gaauaguagg ucuauuauua       60

acaacagcua uggcagcagc cguaaccaga agaggaagug cauauuauau guaccuugac      120

aggaaugaug cgggugaagc aauuaguuuu ccaacaacac uugggaugaa uaaauguuau      180

auucaaauua uggaucuugg ucauaugugu gaugcaacaa ugagcuacga auguccuaug      240

cucgaugaag gaguagaacc agaugaugua gauuguuggu guaauacaac gagcacuugg      300

guaguauaug gaaccugcca ucauaagaaa ggggaagcua gaagaaguag gagagcagua      360

acacugccau cacauucgac aagaaaacua cagacaagaa gucaaacaug guuggaaagu      420

agagaauaca caaaacauuu aauuagagua gaaaacugga uauuuagaaa uccuggauuc      480

gcauuagcag cagccgccau agcuugguug uuaggaagua gcacaaguca aaaagugaua      540

uauuuaguua ugauauuacu aauagcuccu gcauauagua uaagaugcau aggaguaagu      600

aauagagacu uuguugaagg uauguccggg ggaacauggg uagacauagu acuagaacau      660

ggaggaugug uuacaguuau ggcacaagau aaaccuacag uggauauuga acuuguaacu      720

acaacggucu caaauauggc agaaguacga ucauauuguu acgaagcaag uauaucugau      780

auggcaucug acucaagaug cccaacgcaa ggagaagcau accuugauaa acaaagugau      840

acacaauacg uaugcaaaag aacucuagua gacagaggau ggggaaaugg cuguggauua      900

uucggcaaag gaucucuugu uacaugcgca aaauuugccu gcucgaagaa aaugacagga      960

aaaaguauuc aaccagaaaa uuuagaauau agaauaaugu uaagugugca cgguucacaa     1020

cauuccggua ugaucguaaa cgauacagga caugaaacag augaaaauag agcuaaagua     1080

gagauuacac caaauagucc acgagccgaa gcaacacucg gcggauuugg caguuuaggu     1140

uuagauugcg aaccgagaac agguuuagau uuuucagauu uauauuauuu aaccaugaac     1200

aauaaacauu gguuagucca uaaagaaugg uuucacgaca uaccucuacc auggcaugca     1260

ggagcagaua cagguacccc ucauuggaau aauaaagagg cuuuaguaga auuuaaagau     1320

gcacacgcaa aaagacaaac agugguugua uuagguagcc aagaaggugc aguacauaca     1380

gcucuagcag gagcauuaga agcggaaaug gauggugcaa aaggaagauu aaguucaggg     1440

cacuuaaaau guagauuaaa gauggacaag uuacgauuaa aaggaguauc auauagccua     1500

uguacagcgg cauuuacauu uacuaaaaua ccagcagaaa cacuacacgg aacaguaaca     1560

guugaaguac aauaugcugg aacagauggc cccuguaaag uaccagcaca aauggcagua     1620

gacaugcaaa cacuaacccc aguaggaaga uuaauaacag caaauccggu aauuacugaa     1680

ucuacugaaa auagcaaaau gaugcuagaa uuagauccac cauucggcga cagcuauaua     1740

gucauagggg ugggagaaaa gaaaauuaca caucauuggc acagaucagg aaguacuauc     1800

gguaaagcau uugaagcaac aguaagagga gcuaaaagaa uggcaguacu aggggauaca     1860

gcaugggacu uuggaagugu cgguggagca cuaaauucuu ugggaaaagg gauacaccaa     1920

auauuuggug cagcauuuaa aaguuuauuc gguggaaugu cuugguucag ucagauacua     1980

auuggcacuc uuuuaaugug guuaggucua aauacgaaaa augggucaau aucucuaaug     2040

ugucuagcau uaggaggugu uuuaaucuuc uuaagcacag caguaucagc agacguagga     2100

uguugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  9150
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9150
gggagaaagc uuaccaugcg aggacuguca ucaagaaaaa gaagaucaca ugauguacuu       60

acaguacaau ucuuaauauu aggaauguua cuaaugacag gaggcguaac aagaagaggu      120

agcgccuacu auauguaucu agauagaaau gaugcaggag aagcaaucag uuucccuaca      180

acccuuggua ugaauaaaug cuauauacaa auaauggauu uggggcacau gugugacgcu      240

acaaugaguu acgaaugucc aauguuagau gaaggagucg aaccagauga uguagauugc      300

ugguguaaca ccacaucaac auggguggua uacgggacau gccaucauaa gaaaggagaa      360

gcaagacguu caagacgagc uguaacauua ccaagucaua gcacaagaaa auuacaaacu      420

agaucacaaa caugguuaga aucccgagag uacacaaaac aucuaauaag aguggaaaau      480

uggauuuuua gaaauccagg uuucgcauua gcugcagccg cuauagccug guuauuaggu      540

ucaucuacaa gucaaaaagu aauauauuua guaaugauau uacuaauagc accagcauau      600

ucuauaagau guauaggagu cucaaauaga gauuucguag aagguaugag cggaggaacu      660

uggguagaua uuguacuaga acaugguggc uguguaacag uaauggcuca agauaaaccu      720

acaguagaua uagaauuagu aacaacgaca guaucaaaua uggcagaagu uagaucuuau      780

uguuacgaag caagcaucuc agauauggca agugacagua gaugcccaac acaaggggaa      840

gcauaucuag acaaacaauc agacacucaa uauguaugua aaagaacuuu aguugaccga      900

ggauggggaa augguugugg acuauucggu aaaggaucau uaguuacuug ugcgaaauuc      960

gccugcagca agaaaaugac aggaaaauca auccaaccag aaaacuuaga auacagaaua     1020

augcuaagug uacauggaag ccagcacagu ggaaugauag uaaaugauac aggccaugaa     1080

acagaugaaa auagagcaaa aguagaaaua acaccaaauu caccgagagc ugaggcaaca     1140

cuaggaggau uugggagcuu aggauuagau ugugaaccaa gaacaggccu agacuuuagc     1200

gauuuauauu acuuaacaau gaauaauaaa cacuggcuag uacacaaaga augguuucau     1260

gacauaccuu uaccauggca ugcaggagca gacacuggua caccacauug gaacaauaaa     1320

gaagcauuag uagaauuuaa agacgcccau gcgaaaagac aaacgguagu aguacuagga     1380

agucaagaag gggcaguaca uacagcuuua gcuggugcau uagaagcuga aauggaugga     1440

gcaaaaggua gacuauccuc aggucacuua aaauguagac uaaaaaugga caaauuaagg     1500

uugaaaggag uaucauacag uuuauguaca gcagcauuua cauuuacuaa aauaccugca     1560

gagacacugc auggaaccgu uacaguggaa guacaauacg caggaacaga uggaccaugu     1620

aaaguaccag cacaaauggc gguagauaug caaacacuaa ccccgguagg uagauuaaua     1680

acagcaaacc cugugauaac agaaagcacu gaaaacucaa agaugauguu agaauuagac     1740

ccaccauucg gugauaguua uauaguaaua ggagucggag aaaagaaaau aacacaucac     1800

uggcauagaa gugguucaac aauagguaaa gcguucgaag caacaguucg aggagcaaaa     1860

agaauggccg uacuaggaga uacugcaugg gacuuuggau caguaggagg agcuuuaaac     1920

ucucuaggua aagguauuca ucaaauauuc ggggcugcuu uuaagagcuu auuuggcgga     1980

augucuuggu uuucacaaau auuaauagga acacuauuaa uguggcuagg acuaaauacg     2040

aaaaauggaa gcauaagucu gauguguuua gcacugggag gaguacucau cuuccuaagc     2100

acagcaguuu cagccgaugu aggauguuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9151
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9151
gggagaaagc uuaccaugcg aggacuauca ucaagaaaaa gaagaucaca ugacguccuu       60

acaguacaau uuuuaauauu aggaauguua uuaaugaccg gagcaguaac uagacgagga      120

ucagcauauu acauguaccu agaccgaaau gacgcaggag aagcuauauc auuuccuaca      180

acccuaggaa ugaacaaaug uuacauacaa auaauggauu uaggacauau gugugaugcc      240

acaaugucau augaaugucc aaugcucgau gaaggaguag aaccggauga cgucgauugu      300

uggugcaaca caacuucgac uuggguagua uacgguacuu gucaucauaa gaaaggagaa      360

gcgagacguu caaggagagc agugacacua ccgucucacu caacaagaaa auuacaaaca      420

agaucacaaa cgugguuaga aagcagagaa uauacaaaac acuuaaucag aguggaaaau      480

uggauauuua ggaacccagg cuucgcuuua gcagcggcag caauagcaug guuacuagga      540

uccagcacau cacaaaaagu aauauacuug guaaugauau uacuaauugc uccagcauac      600

ucaauuagau guauaggugu aaguaacaga gauuucguug aaggaaugag uggaggaaca      660

ugggucgaua uaguacuuga gcauggcgga ugcguaaccg uaauggcaca agauaagcca      720

acuguagaua uagaacuggu uacaacaaca gucucuaaua uggcagaagu aagaagcuau      780

uguuaugaag cgucaauauc agauauggcg ucagacucaa gaugccccac ccaaggagaa      840

gcauaucuag auaaacaaag ugacacacaa uauguaugca aaaggacucu aguagauaga      900

ggauggggaa augggugugg auuauucgga aaaggguccc uaguaacaug cgcaaaauuu      960

gccugcucaa agaaaaugac agggaaauca auucaaccag aaaaccuaga auacagaaua     1020

auguuaagcg uucauggaag ucaacauucg gguaugauug uaaacgauac aggacaugaa     1080

acugacgaaa auagagcgaa aguugaaaua acaccuaauu caccaagagc agaagcaacu     1140

cucggaggau ucggaagucu uggacuggau ugcgaaccac gaacaggguu agauuuuagc     1200

gaccuauauu aucuaacgau gaauaacaaa cauugguuag uacacaaaga augguuucau     1260

gacaucccac uaccauggca ugcaggagca gacacaggua cgccacauug gaauaacaaa     1320

gaagccuuag uagaauuuaa ggaugcacau gcaaaaaggc aaacuguagu ugucuuagga     1380

agccaagaag gcgcaguaca uacagcauua gcuggagcuu uagaggcuga aauggaugga     1440

gcaaaaggua gguuaucaag uggacauuua aaaugucgau uaaaaaugga uaaauuaagg     1500

uuaaaaggag uaucuuauuc auuaugcaca gcagcauuua cauuuacaaa aauaccagca     1560

gaaacccuac augguacagu aacaguagaa guccaauaug caggcacaga uggaccaugc     1620

aaaguuccag cucaaauggc cguagauaug caaacuuuaa cacccguugg aagacuaaua     1680

acagcaaauc caguaauaac agaaucaaca gaaaauagua aaaugaugcu agaacuagau     1740

cccccauuug gcgauagcua uauagucaua ggggucggag aaaagaaaau uacacaucac     1800

uggcaucgau caggcaguac aauagguaaa gcauuugaag caacaguacg aggagcuaaa     1860

agaauggcag uccuaggaga uacagcuugg gacuuugguu caguaggagg agcauuaaac     1920

ucauuaggaa aagguauaca ucaaauauuu ggagcagcuu ucaaaucauu auuuggaggc     1980

augucuuggu uuagucagau acuaauagga acucuauuaa ugugguuagg cuuaaauacu     2040

aaaaauggaa gcauaucuuu aaugugucua gcuuuaggug gaguauuaau auuuuuaagc     2100

acagcuguau cagcugaugu aggcuguuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9152
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9152
gggagaaagc uuaccaugag gauaauaaau gcccgcaaag aaaagaaacg gagaggagcc       60

gauacaucug uagguauugu aggauuauua uuaacaacag cgauggcagc agaaguaaca      120

agaagaggau cagcuuauua uauguauuua gacagaaacg augcaggaga agcaauuagc      180

uuuccaacua cauuagguau gaauaaaugu uauauacaaa uaauggauuu aggacauaug      240

ugugaugcga caauguccua ugaaugucca augcuagaug aagguguaga gccagaugau      300

guagauugcu gguguaauac aacaucaaca ugggucguau auggaacaug ccaucauaag      360

aaaggugaag caaggcguag uaggagagca guuacauuac caucucauuc uacuagaaaa      420

cuacaaacaa gaucacaaac augguuagaa agucgagaau auacuaaaca uuuaauaaga      480

guagaaaacu ggauuuuuag aaacccagga uuugcacuag cugcagcugc aauagcaugg      540

uuguugggaa gcaguacaag ucagaaagua auuuauuuag uaaugauauu auuaauugca      600

ccagcauauu caauuagaug uauaggugua ucuaacagag acuuuguaga gggcauguca      660

ggaggaacau ggguugauau uguuuuagaa caugguggau guguaacugu aauggcucaa      720

gauaaaccaa caguagacau cgaauuagug acuacaacag uaucaaacau ggcugagguu      780

agauccuauu guuaugaagc cucaaucagc gauauggcca gugauaguag auguccaaca      840

caaggugaag cauauuuaga uaaacaaagu gauacgcaau auguuuguaa aaggacguua      900

guagaucggg gcuggggaaa cggaugcggc uuauuuggaa aaggaaguuu aguaacaugu      960

gcaaaauuug caugcucaaa gaaaaugaca gguaaaagca uucagccuga aaauuuagaa     1020

uauagaauaa ugcuaagugu acauggcagu caacauucag gaaugauugu aaaugacacg     1080

ggacacgaaa cagacgaaaa cagagcaaaa gucgaaauaa cgccuaauag uccaagagca     1140

gaagcaacuu uagguggauu uggaagccuu ggacuagauu gugaaccacg aacugguuug     1200

gauuucagug auuuauacua uuuaacuaug aauaacaaac acugguuagu acauaaagaa     1260

ugguuucaug acaucccauu accauggcau gcaggagcgg auacugggac accacauugg     1320

aacaauaaag aggcguuagu cgaguucaaa gaugcacaug cgaaacgaca gacaguagua     1380

guauugggaa gucaagaagg agccguacac acagcauuag cuggagcauu agaagcugaa     1440

auggacggag caaaaggaag auuaucaucu ggacacuuga aauguagauu aaaaauggau     1500

aaacucagac ugaaaggagu aagcuacagu cuauguacag cagcauucac auuuacaaaa     1560

auuccugcag aaacacuaca uggaacaguu acaguagaag uacaauaugc aggaacagau     1620

ggaccaugua aaguaccggc acaaauggcu guagacaugc aaacacuuac gccuguagga     1680

agguuaauua cagcaaaccc ugugauaaca gaaaguacgg aaaauucaaa aaugauguua     1740

gaacuagacc cuccauucgg ugauaguuau auagucauag gaguagguga aaagaaaauu     1800

acacaccauu ggcaucgcuc aggcucaaca auaggaaaag cguuugaagc gacaguaagg     1860

ggagcaaaaa gaauggcagu guuaggggau acagcauggg auuucggauc gguaggaggu     1920

gcauugaaua gucuaggaaa agguauucac caaauauuug gagcagcguu caaaucauua     1980

uuugggggua ugaguugguu cucacaaaua uuaauaggga ccuuauuaau guggcuagga     2040

cuaaauacga aaaaugguuc aauuagucuc auguguuuag cauuaggugg aguauugaua     2100

uuccuaagua cagcaguauc cgcagauuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9153
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9153
gggagaaagc uuaccaugau aauuaacgca agaaaagaaa aaaagagaag gggggcagac       60

acaucuguag gaaucguagg guuauuauua acaacagcua uggcagccga aguaacgcga      120

agaggaucag cuuauuauau guauuuagac agaaacgaug caggagaagc aauaaguuuu      180

ccaacuacuc uaggaaugaa uaaauguuac auacaaauaa uggauuuagg acacaugugu      240

gacgcaacaa ugagcuauga augcccaaug uuggaugaag gcguagagcc cgaugaugug      300

gauuguuggu guaauacaac aagcacuugg gugguauaug gcacuugcca ucacaaaaaa      360

ggcgaagcaa gaaggucuag aagagcagua acauuaccau cccacucaac aagaaaauua      420

caaacaagau cccaaacaug gcuagaaucu agagaauaua caaaacauuu aauucgcgua      480

gaaaauugga uauucagaaa uccgggguuu gcacuagcag cagcugcaau agcauggcua      540

uuaggaucau cgacaagcca aaaaguaaua uauuuaguaa ugauacuacu cauagcaccu      600

gcauacucaa uaagauguau cgguguauca aauagagauu ucguggaagg caugaguggu      660

ggaacauggg uugacauagu uuuagaacau ggagguugug uuacuguaau ggcucaagau      720

aaaccaacag uagauauaga acucguaaca acaacaguuu caaauauggc ggaaguaaga      780

aguuauuguu augaagcuuc uauuucagau auggccagcg acagucguug cccaacacaa      840

ggugaagcau aucuagacaa gcaaagugau acucaauaug uaugcaaaag aacacuagua      900

gacagagguu gggguaaugg augugguuua uuuggaaaag gaagccuagu aacaugcgca      960

aaauuugcuu gcaguaaaaa aaugacaggg aaaaguauac agcccgaaaa ccucgaguau     1020

agaauaaugu uaucuguaca uggaucacaa cacagcggaa ugauaguaaa ugauacagga     1080

cacgaaacag augaaaacag agcaaaaguc gaaauaacac caaauagucc ucgagcugaa     1140

gcuacauuag gaggauucgg aucgcuaggg uuagauugcg aaccuagaac uggauuagac     1200

uuuucugauu uauauuaccu gacaaugaac aacaaacacu ggcuuguaca uaaagaaugg     1260

uuccaugaua uuccauuacc auggcaugcc ggagcagaua caggaacccc ccauuggaau     1320

aauaaagaag cauuaguaga auucaaagac gcgcacgcua aaagacaaac aguaguagua     1380

cuaggaucac aagaaggagc uguacauaca gcauuggcag gagccuuaga agccgaaaug     1440

gacggagcaa aaggucgucu uucaagcgga cauuugaaau guagacuaaa aauggauaaa     1500

cucagacuaa aagggguauc auauucacua uguacagcag cauuuacauu uacaaaaaua     1560

ccagcagaaa cauuacaugg uacagucaca guagaaguuc aauaugcagg aacagauggu     1620

ccguguaaag uaccugcaca aauggcagua gauaugcaga cauuaacacc gguugguagg     1680

uuaauuaccg caaauccagu aauaacagaa aguacagaaa auucaaaaau gauguuagaa     1740

uuagauccac cauuuggaga cagcuauaua guaauaggag uaggugaaaa aaaaauaaca     1800

caucauuggc auagaagugg aucaacaaua ggaaaagcau uugaagcaac ggucagagga     1860

gcaaaaagaa uggccguuuu aggggauaca gcaugggauu uuggaagugu agguggagca     1920

uuaaacucuu uagguaaagg cauacaucaa auauuuggag cggcuuuuaa aaguuuauuc     1980

ggugguaugu caugguuuag ucaaauacua auaggaacuu uauuaaugug guugggauua     2040

aauacaaaaa acgguucgau uaguuuaaug ugcuuagcau uaggcggagu auuaauauuc     2100

uuaaguacgg cgguaagugc ugauugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  9154
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9154
gggagaaagc uuaccaugau aaaugcaagg aaagaaaaaa aaagaagagg agcagauaca       60

uccguuggaa uaguuggacu gcuauuaaca acagcuaugg cagcagaggu uacacgaaga      120

ggaagcgcau auuauaugua ucuagauaga aaugaugcag gagaagcuau aaguuuuccg      180

acaacauuag gaaugaauaa auguuauaua caaauaaugg accuaggaca uaugugugau      240

gcaacaauga guuaugaaug uccaauguug gacgaaggag uagaacccga cgacguagau      300

uguuggugua auacaacaag caccugggua guauauggca caugucacca uaagaaagga      360

gaagcaagaa gaucacgaag agcgguaaca uuaccaucac auucuacacg aaaauuacaa      420

acaaggucac aaacaugguu agaaagcaga gaauauacaa aacauuuaau ucgaguggaa      480

aauuggauau uuagaaaucc aggauuugca cuagccgcag cagcaauagc augguuacua      540

ggaucaucaa caucacaaaa aguaauauau uuaguaauga uacuacuuau agcaccagcc      600

uacucaauua gaugcauugg aguaucuaau agagauuucg uagaaggaau gagcggagga      660

acuugggugg auauuguauu agaacacgga ggauguguaa caguaauggc ucaagauaag      720

ccaacaguag acauagaauu aguaacaacu acaguaagua auauggccga ggucagaagc      780

uacugcuaug aagcaucaau uucagauaug gcaagcgauu caagaugucc aacacaaggu      840

gaagcuuauu uggacaaaca aagugacaca caauauguau gcaaacgaac acugguagau      900

cgcggauggg gaaauggaug uggauuauuu ggaaaagggu cauuaguaac augugcaaaa      960

uucgccuguu caaaaaaaau gacuggaaaa uccauucaac ccgaaaaucu agaauauaga     1020

auaauguuaa guguacaugg aucacaacau ucugguauga uaguaaauga uacaggucau     1080

gaaacagaug aaaaccgcgc aaaaguagaa auaacaccua acucaccuag agcagaagca     1140

acacuaggag gauuuggaag cuuaggguug gacugugaac cuagaacagg acuggacuuu     1200

ucugacuuau auuaucuaac aaugaacaau aaacauuggu uaguacauaa agaaugguuc     1260

caugauauac cauuaccaug gcaugcagga gcugauacag gaacaccuca cuggaacaau     1320

aaagaagcac ucguagaauu uaaagaugcc caugcgaaaa gacaaacugu aguaguuuua     1380

ggaucccaag aaggcgcugu acacacugca cuagcugggg cauuagaagc cgaaauggac     1440

ggugcaaagg gacgauuauc auccggacau uugaaauguc guuuaaaaau ggacaaauua     1500

agacuaaaag gaguaucgua uucacuaugu acagcagccu uuacuuuuac aaaaauacca     1560

gcagaaacau uacauggaac aguuacaguu gaaguacaau augccggaac agacggacca     1620

ugcaaaguuc cagcacaaau ggcuguagau augcaaacau uaacuccagu aggcagauug     1680

auaacagcaa acccugucau aacagaauca acugaaaaca guaaaaugau gcuugaacua     1740

gaccccccau uuggagacuc auacauagua auagggguag gugaaaaaaa aauaacacac     1800

cauuggcaua gaaguggauc cacaauaggu aaagccuuug aagcaacugu acguggagca     1860

aaaagaaugg cagucuuagg ggauacagca ugggauuuug gaucaguagg aggagcuuua     1920

aacucuuuag gaaaaggaau acaucaaaua uuuggagcag cauucaaauc acuuuuugga     1980

ggaaugucau gguuuucuca aauucuaauc gggacacuac uuauguggcu aggauuaaau     2040

accaaaaacg gcaguauauc acugaugugu uuggcauuag guggaguuuu aauauuuuug     2100

agcacagcgg ugucagcaga uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9155
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9155
gggagaaagc uuaccaugaa ugcgaggaaa gaaaagaaaa gaagaggugc agauacauca       60

gucggaauag uaggacuguu auugacaaca gcgauggcag cagaaguaac aagaagagga      120

agcgcauauu auauguacuu agauagaaac gaugcuggug aagcaauauc auuuccuaca      180

acauuaggaa ugaacaaaug uuauauacaa auaauggauu ugggacauau gugugaugcu      240

acaaugaguu augaaugucc aauguuggau gaaggaguag agccagacga uguagauugu      300

ugguguaaca caacaucaac auggguagua uaugggacuu gucaccauaa gaaaggggaa      360

gcucgacgaa gccguagagc gguaacgcua ccaagccaua gcacaagaaa auuacaaacg      420

agaagucaaa cuugguuaga aagcagagaa uacacaaaac auuuaauaag aguagaaaau      480

uggauauuca gaaacccagg guuugcacua gcggcggcug caauagcaug gcuccuagga      540

ucaucaacau cacagaaagu aauauaccua guaaugauac uacuaauagc uccugcauau      600

aguaucagau guauaggagu aaguaaucga gauuuuguag aagguauguc cgguggaacg      660

uggguagaca uaguacuaga acauggagga uguguaacag ucauggcaca agacaaaccg      720

acaguagaca uagaauuagu uacgacaaca guaucaaaua uggcagaagu gagaucuuau      780

uguuacgaag caagcauaag cgacauggca agugacagcc guuguccaac acaaggagaa      840

gcauacuuag auaaacaaag ugauacacaa uauguaugua aaagaacgcu aguugacagg      900

ggcuggggaa acggaugcgg cuuauucgga aaaggaucuc uaguuacaug ugcaaaauuu      960

gcauguucaa agaaaaugac aggcaaauca auacaaccgg aaaauuuaga auauaggaua     1020

auguuaucag ugcaugguuc acaacauuca ggaaugauag uuaacgauac cggucaugag     1080

acagacgaaa auagagcaaa aguagaaaua acacccaacu caccaagagc agaagcaaca     1140

cuagguggau ucgguagcuu aggauuagac ugugaaccaa ggacaggauu ggauuuuagu     1200

gaccuauauu accuaacaau gaauaauaaa cacuggcuag uacauaaaga augguuucau     1260

gauauaccgu uaccuuggca ugcuggugcu gauacaggga cuccacauug gaauaauaaa     1320

gaagcauuag uagaauuuaa agaugcgcac gcgaaaagac aaacgguagu aguauuaggg     1380

ucacaggaag gagcaguaca uacugcacua gcaggagcau uagaagcaga aauggaugga     1440

gcuaaaggaa gacucagcuc agggcacuua aaaugcagau ugaaaaugga uaaauuaaga     1500

cuuaaaggug uuaguuauuc ucucuguacu gcagcauuua cuuucacuaa aauaccggca     1560

gaaacacuuc auggcacagu aacagucgaa guacaguacg caggaacaga ugggccaugu     1620

aaaguaccag cacaaauggc uguagauaug caaacguuaa caccuguagg acgauuaaua     1680

accgcaaacc ccguaauuac agaaucaaca gaaaacucaa aaaugaugcu ugaauuagac     1740

ccaccauucg gagauagcua uauaguaaua ggagugggag aaaagaaaau aacacaucau     1800

uggcauagaa gcgguucuac aauagguaaa gcauucgagg caacgguuag aggggcaaaa     1860

agaauggcgg uauuaggaga cacagcuugg gauuucggaa gcguaggugg agcauuaaau     1920

ucacuuggaa aagguauaca ucaaauauuu ggagcagcau ucaaaaguuu auucggaggu     1980

augucauggu uuagucaaau uuugauagga acauuacuga ugugguuagg guuaaauacu     2040

aaaaacggaa gcauuagcuu aaugugucuu gcucuaggag gcguauuaau auucuuaagc     2100

acagcaguua gugcagacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9156
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9156
gggagaaagc uuaccauggc uagaaaagaa aagaaacgaa gaggcgcaga uacaucagua       60

ggaauaguug gucuauuacu aacaacugca auggcagcag aaguaaccag aaggggaagu      120

gccuauuaua uguaucuuga uaggaaugau gcuggugagg caauaucauu uccuacaacu      180

cuuggaauga auaaaugcua cauacaaaua auggauuuag gucauaugug cgaugccaca      240

augucauacg aaugcccaau gcuagaugaa ggaguagaac cugaugaugu agacuguugg      300

ugcaacacaa ccaguacaug gguaguauau ggaacuuguc aucacaagaa aggagaagca      360

agaagaagua gacgagcagu aacgcuacca agccacucaa cacggaaauu acaaacaaga      420

agccaaacau gguuagaaag cagagaguau acgaaacacc ucaucagagu agaaaacugg      480

auauuuagaa acccgggauu ugcauuagca gcagcggcaa uagcauggcu auuaggaagu      540

uccacaaguc aaaaaguuau cuacuuagua augauacuuu ugauagcacc agcauacuca      600

auuagaugua ucgggguuuc uaauagagau uuuguagaag gaaugagugg agguacaugg      660

guagauauag uauuagaaca cggaggaugu gucacaguaa uggcacaaga uaaaccaaca      720

guugacauag aauuagugac aacaacagua agcaauaugg cagaaguacg auccuacugu      780

uacgaagcaa guauaagcga uauggcuagu gacucacgau guccuacuca aggugaggcu      840

uaccuagaua aacaaagcga uacgcaguau guaugcaaaa gaacauuagu agacagggga      900

ugggguaacg gaugcgggcu auuuggaaaa ggcucucuug uaacaugugc aaaauucgcu      960

uguaguaaga aaaugacggg uaaaucaaua caaccagaaa aucuagaaua uagaauaaug     1020

uuaaguguac auggaucuca acauaguggg augaucguaa augauacagg ucacgaaaca     1080

gacgaaaaca gagcuaaagu agaaauaaca ccaaauagcc cgagagcaga agcuaccuug     1140

ggaggauuug gaagccuagg ucuugauugu gaacccagaa caggauuaga uuucucagau     1200

uuauauuacc uaaccaugaa uaauaaacau uggcugguac auaaagaaug guuucaugau     1260

auaccuuuac cauggcacgc gggggcagac accgguacuc cacacuggaa uaauaaagag     1320

gcauuaguag aauuuaaaga cgcacaugca aaaaggcaaa caguaguggu acuagguagc     1380

caagaaggag caguacauac cgcauuagca ggagcacuag aggcugaaau ggauggagca     1440

aaaggaagac uaucaucagg acaucuuaaa ugucgauuaa aaauggacaa auuaagauua     1500

aaagggguaa gcuauaguuu augcacggca gcuuuuacau uuaccaaaau accagcagaa     1560

accuuacaug guaccguaac cguugaaguu caauaugcug gaacagacgg accauguaaa     1620

guacccgcac aaauggcagu agauaugcaa acacuaacac caguugggcg acugauaacu     1680

gcaaauccag uaauaacaga gucaacagaa aacucaaaga ugauguuaga auuagaucca     1740

ccauuuggug auucauauau aguaauagga guuggagaaa agaaaaucac acaucauugg     1800

cauagaagcg gaaguacuau aggaaaggcu uuugaagcaa caguaagagg agcaaaacga     1860

auggcagucu uaggagauac ugcuugggau uucggaagug uaggaggagc uuuaaacagc     1920

cucgguaaag ggauacauca aauauuugga gcagcauuua aaaguuuauu uggaggaaug     1980

aguugguuuu cacaaauauu aauuggcaca uuacuuaugu ggcuagguuu aaauacgaaa     2040

aacggaagca uauccuugau gugcuuagca cuuggagggg uacuaauauu cuugaguaca     2100

gcaguaagug cagauugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9157
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9157
gggagaaagc uuaccaugag aaaagagaaa aaacgaagag gcgcagauac uaguguaggc       60

aucguaggac uauuauuaac aacugcuaug gcagcagaag uuacaagaag aggcagcgca      120

uauuacaugu auuuagaccg aaaugacgca ggagaagcua uuucauuccc aacaacauua      180

ggaaugaaca aauguuauau acaaauuaug gacuuaggcc acauguguga ugcaacaaug      240

ucauaugaau guccaauguu agaugaagga guagaaccag augauguaga cuguuggugc      300

aauacaacaa guacaugggu aguauacggu acaugucauc auaaaaaagg ugaagcaaga      360

agaucuagga gagcaguaac cuuaccauca cauucaacaa gaaaauuaca gacaagaagu      420

caaacguggu uagaaaguag agaauauaca aaacaucuaa uuaggguaga aaauuggaua      480

uucagaaauc cgggauuugc auuagcagcc gcagcaauag cauggcuauu agggucuuca      540

acaagucaga aaguaauuua ccuaguaaug auauuacuaa uagcaccggc cuacaguaua      600

cgguguauag gaguaaguaa cagggauuuc guugaaggaa ugaguggagg uacaugggua      660

gacauaguau uagaacaugg uggaugugua acuguuaugg cacaagauaa accuacagua      720

gacauugaac uaguuacaac aacaguuagu aacauggcag aaguaaggag uuauuguuau      780

gaagcuucaa uaagugauau ggcaucagau ucacguuguc cuacacaagg agaagcuuau      840

uuagauaaac aaagugauac acaauacgua uguaaaagaa cauuaguaga ucgaggaugg      900

ggaaaugguu guggucuauu cggcaaaggu ucuuuaguaa ccugugcaaa auuugcuugc      960

agcaaaaaaa ugacuggaaa aaguauccaa ccagagaacu uagaauauag aauaauguua     1020

aguguacaug gaagccaaca cucaggaaug auaguaaaug auacaggaca ugaaaccgau     1080

gagaauagag cuaaaguaga aauaacacca aacucaccuc gagcugaagc aacauuagga     1140

ggauucggau cguugggauu agacugugaa ccgagaacug gacuagauuu uucugauuua     1200

uauuauuuaa caaugaauaa uaaacacugg uuaguucaua aagaaugguu ucaugauaua     1260

ccauuaccuu ggcaugcagg ugcugauacu ggaacaccac acuggaauaa uaaagaagca     1320

uuaguagaau uuaaggaugc acacgcgaaa agacaaacag uaguaguuuu aggaagccaa     1380

gagggagcag uacacacagc ucuagcagga gcguuagaag cagaaaugga uggugcaaaa     1440

ggacgauuau caucaggaca ucuuaaaugu cgacuaaaaa uggacaaacu gagacuaaaa     1500

ggaguaucau auaguuuaug uacagcagcg uuuacauuua cuaaaauacc ugcggaaaca     1560

cuucauggaa cuguuacggu agaaguacaa uaugcaggaa cugauggacc augcaaagua     1620

ccggcccaaa uggcaguaga caugcaaacu uuaacaccag ucggaagauu aauaacugca     1680

aaccccguaa uaacagaaag cacugaaaac aguaaaauga uguuagaacu ugauccccca     1740

uucggagaua gcuauauagu uauaggcgua ggagaaaaga aaauaacaca ucauuggcac     1800

agaucaggau caacaauagg aaaagcuuuu gaagcuacag ugagaggagc aaaaagaaug     1860

gcaguauugg gagauacagc uugggauuuc gguucuguug gcggagcgcu aaauagucua     1920

ggaaaaggaa uacaucaaau auucggugcu gcauuuaaau cauuguucgg aggcauguca     1980

ugguuuagcc aaauuuuaau aggaacacua uuaauguggu uaggauuaaa caccaaaaau     2040

ggcucuauuu cacuaaugug cuuagcauua ggagguguau uaauauucuu aaguacagcg     2100

guuagugcgg acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  9158
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9158
gggagaaagc uuaccaugaa agagaaaaaa agaagaggag cagauacguc aguaggaauu       60

guaggauuau uauugacaac agcaauggca gcugaaguga cucguagagg aucagcauac      120

uauauguauu uggaucguaa ugacgcaggu gaagcaauuu cuuuuccuac aacauuaggu      180

augaauaagu guuauauaca aauaauggac uuaggacaca ugugugaugc aacaauguca      240

uaugaaugcc cuauguuaga cgaaggggua gaaccagacg auguugauug uugguguaau      300

acuacaucaa cauggguagu auacggaaca ugucaccaua aaaaaggaga agcacgcaga      360

aguagacgag caguaacacu accuucacau ucaacaagaa aacuacagac gagaucacaa      420

acuuggcuag aaaguagaga auacacaaaa caucuaauaa gaguagaaaa uuggaucuuu      480

agaaauccag guuuugcguu agcagcagca gcaauagcuu gguuguuagg gucgucaacu      540

agccaaaaag uaauauaucu aguaaugaua uuauuaaucg caccggcuua uaguauaaga      600

ugcauaggag uuaguaaucg agauuuugua gagggaaugu caggaggaac uuggguagau      660

aucguucuag aacacggagg auguguaaca guaauggcac aggauaaacc aacaguugau      720

auugaauuag uaacaacaac aguaagcaac auggcagaag uaaggaguua uugcuacgaa      780

gcuaguauau cagauauggc aagugauuca agauguccua cacaagguga agcauauuua      840

gauaaacaaa gcgacacaca auaugucugu aaaagaacau ugguugauag agguugggga      900

aaugguugcg gauuauucgg caaagguucu cuaguaacuu gcgcaaaauu ugcauguuca      960

aaaaaaauga cagguaaaag cauacaacca gaaaacuuag aauauaggau uauguuaucg     1020

guucauggcu cgcaacauag cgguaugauu guaaaugaua caggacacga aacagacgaa     1080

aacagagcaa aaguagaaau aacuccaaau agcccaagag cagaagccac acuaggugga     1140

uuugguaguu uaggacuaga uugugaacca agaacaggau uagauuuuag ugaccuauau     1200

uauuuaacaa ugaauaauaa acacugguua guucauaaag aaugguuuca cgauauucca     1260

uuaccuuggc augcaggugc ggacacagga acgccacauu ggaacaauaa agaagcuuua     1320

guagaauuca aagaugcuca ugcaaaaaga caaacaguag uaguacuagg aagucaagaa     1380

ggagcgguac auacagcacu agcaggugca uuagaagcag aaauggaugg agcgaaaggc     1440

agacuaucau cuggacaucu aaaauguaga uuaaaaaugg acaaacuaag gcuaaaagga     1500

guaaguuacu cacuauguac agcagcauuc acauuuacaa aaauuccagc cgaaacguua     1560

cacggaacag uaacggucga agugcaauau gcaggaacug auggacccug uaaaguaccu     1620

gcucaaaugg caguagauau gcaaacacuu acaccaguag gaagauuaau aacagcuaau     1680

ccaguaauaa cagaaucuac agagaacuca aaaaugaugc ucgaauuaga cccaccauuc     1740

ggugauaguu auauaguaau aggaguagga gaaaagaaaa uaacacauca cuggcacaga     1800

ucagguucaa caauuggaaa ggcauuugaa gcuacuguua gaggagcaaa acgaauggca     1860

guauugggug acacggcaug ggauuuugga ucaguaggag gagcuuugaa uucucuaggc     1920

aaaggaauac accaaauauu cggagcagcc uucaaaucuc uauuuggagg uaugagcugg     1980

uucucacaaa uauuaauagg uacuuuauua augugguuag gauuaaacac uaaaaaugga     2040

ucaauauccc uaauguguuu agcguuagga gguguauuaa uauuccuaag uacagcuguc     2100

agcgccgauu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9159
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9159
gggagaaagc uuaccaugga aaaaaaaaga cgaggggcgg auacaucagu aggaauaguu       60

gguuuauuau uaacaaccgc aauggcagca gaaguaacua gaagaggauc agcauauuau      120

auguauuuag auagaaauga ugcaggagag gcaauaucau uuccuacaac acuuggaaug      180

aauaaauguu acauacagau uauggauuug ggacacaugu gugacgcaac aaugaguuac      240

gaaugcccaa ugcuagauga agggguagaa ccagacgacg uggauuguug guguaacaca      300

accucaacgu gggucguaua cggaacaugc caccauaaaa aaggagaagc ccgacggagu      360

cgaagagcag ugacauuacc aagucauuca acgagaaaau uacaaacuag aagccaaaca      420

ugguuagaaa gccgagaaua cacaaaacau cuuauucgag uagaaaauug gauauuuaga      480

aaucccgggu uugcacuagc agcagcagca auagcauggu uacuaggauc aucaacaucc      540

caaaaaguga ucuacuuagu aaugauauug cuuauagcgc cagcauauag uauaaggugu      600

aucggaguaa guaauagaga uuucguagaa gguaugagug gaggaacaug gguugacaua      660

guuuuggagc auggcgggug cguaacagua auggcacaag acaaacccac gguagacaua      720

gaauuaguga cuacuacugu aucaaauaug gcagaaguua gaagcuacug uuacgaagca      780

ucaauaucug acauggcaag ugauaguaga uguccaaccc aaggagaagc auaucuagau      840

aaacaaucag auacacaaua cguauguaaa agaacucuug uagaucgagg uuggggaaau      900

gguugcggcu uauuuggaaa aggaaguuua guaacaugug caaaauuugc auguucuaaa      960

aaaaugaccg guaaaucgau acagccggaa aaccuagaau accgaauuau guuaagugua     1020

caugguucgc agcauucagg aaugauagug aacgacacag gucaugaaac agaugaaaau     1080

cgugcuaagg uagaaauuac accaaacucg ccaagagcag aagcaacacu aggaggauuu     1140

gguucacugg gauuggauug ugaaccuaga acaggccuug auuucucaga uuuauauuau     1200

uuaacaauga auaauaaaca uuggcuugua cauaaagaau gguuucauga uauaccauua     1260

ccauggcacg cuggagcaga cacggguaca ccacauugga acaacaaaga agcacuagua     1320

gaauuuaagg acgcccaugc aaaaagacaa acugugguag uacuagguag ucaagaagga     1380

gcuguacaua cugcucuggc aggggcauua gaagcugaaa uggauggugc aaaaggaaga     1440

uuaucaagug gacacuuaaa augcagacua aaaauggaca aauuaagauu aaaaggaguu     1500

ucauauaguu uauguacagc agcauuuacu uuuacaaaaa uuccagcaga aacauuacau     1560

ggcacaguaa cuguagaagu ccaauaugca ggaacagacg gaccuuguaa aguuccagca     1620

caaauggcag uagacaugca aacguuaaca ccaguuggaa gacuuauaac ugcaaaucca     1680

guaauaacag aaaguacaga gaauaguaaa augaugcuag aauuagaucc accuuucgga     1740

gauucauaua uuguaauagg uguaggagaa aaaaaaauaa cacaccacug gcaucgaagu     1800

gguaguacua uaggaaaagc auuugaagcu acagucaggg gagcaaagag aauggccgua     1860

cuaggagaua cagcauggga cuucggaagc guuggaggug cccuaaauuc auuagggaaa     1920

gguauacacc aaauauucgg cgccgcauuu aaaagccuau uuggaggaau gaguugguuu     1980

ucacaaauac uaauaggcac auuauuaaug ugguuaggcu uaaacacaaa aaauggauca     2040

auaagucuaa uguguuuagc auuaggagga guauuaauuu uucuaucaac agcuguaucu     2100

gcugacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  9160
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9160
gggagaaagc uuaccaugaa aaaaagaaga ggagcagaca caaguguagg aauaguagga       60

cuauuauuaa caacugcaau ggcagcagaa guaacaagaa gaggaucagc guauuauaug      120

uaucucgaua gaaaugaugc aggagaggca auauccuucc caacaacucu uggaaugaac      180

aaaugcuaua uacaaauuau ggauuuagga cauaugugcg augcaaccau guccuacgaa      240

uguccaaugu uagaugaagg uguagaaccu gaugauguag auuguuggug uaauacaaca      300

aguacuuggg ugguauaugg aacaugccau cauaaaaaag gagaggcaag aagaagcagg      360

agagcgguaa cacuaccauc ccauagcaca cgaaaacuac aaacuagaag ucaaacaugg      420

cuagaaagca gagaauacac uaaacaucuu auaagaguag aaaauuggau auuuagaaac      480

ccaggguuug cgcuagcagc cgcagccauu gcuugguuau uaggcucauc aacaagccaa      540

aaaguaauau accuaguaau gauacuacua auagcgccug cauauucaau uagaugcauu      600

ggagucucca auagagauuu uguagaagga augaguggag ggacaugggu cgacauagua      660

cuagaacacg gagguugugu uacagugaug gcgcaagaua aaccaaccgu cgauauagaa      720

uuaguaacua caacaguauc aaauauggca gaaguaagau ccuauuguua ugaagcaucg      780

auaagcgaua uggccucaga uucuagaugu cccacacaag gagaagcuua cuuagauaaa      840

caaucugaua cacaauaugu cuguaaaaga acacucguag auagaggaug gggaaaugga      900

uguggccuau ucggaaaagg uaguuuagua acaugugcaa aauuugcaug uagcaaaaaa      960

augacaggaa aaagcauuca accagaaaau cuggaauaua gaauaauguu aucuguacau     1020

ggaagucaac auucagggau gauaguuaau gauacaggac acgaaacaga ugaaaaucga     1080

gcaaaaguug aaauaacacc uaauagucca agagcagaag caacauuagg aggauuugga     1140

agucuaggau uagacuguga accuagaacu ggauuggauu uuagugacuu auauuaucuu     1200

acuaugaaua auaaacacug gcuuguucau aaagaauggu uccacgauau accacuacca     1260

uggcaugcag gcgcugacac agguacaccc cauuggaaua acaaagaagc acuaguagaa     1320

uucaaagaug cucaugcaaa aagacaaaca gucguaguuc uaggaaguca agaaggagca     1380

guacacacag cuuuagcugg agcauuagaa gcagaaaugg auggugcaaa aggacgacua     1440

ucaucaggac acuuaaaaug cagacuaaaa auggauaaau uaagacuaaa aggaguauca     1500

uauaguuugu guacagccgc auuuaccuuu acaaaaauuc cagcugaaac auuacacgga     1560

acuguaacag uggaaguaca auaugcaggu acagacggac cguguaaggu accagcucag     1620

auggcaguag auaugcaaac auuaacgccc guugguagau uaauaacugc aaauccagug     1680

auaacagaau caacagagaa uagcaaaaug augcuagaau uagauccccc auuuggagau     1740

agcuauauag uaauuggagu aggagaaaaa aaaauaacac aucauuggca ucgaucagga     1800

uccacuauag guaaagcuuu ugaagcaaca guaagagggg cuaaaagaau ggcgguauua     1860

ggagauacag ccugggauuu uggaucaguc ggaggugcau uaaacaguuu agguaaagga     1920

auucaucaaa uauuuggagc agcauucaaa agcuuauuug gagguauguc augguuuucg     1980

caaauacuga uuggaacauu gcuaaugugg cuaggacuaa auacuaaaaa uggaaguauc     2040

ucauuaaugu gucuagcacu aggaggugua uuaauauuuc ucagcacagc agucucagca     2100

gauugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  9161
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9161
gggagaaagc uuaccaugaa aagaagaggu gcagauacau caguaggaau aguuggguua       60

uuacuaacaa cagcaauggc agcagaagua acgagaaggg guagugcgua cuauauguac      120

cuagaccgaa augaugcagg ugaagcuaua agcuuuccaa ccacacuagg caugaauaaa      180

uguuauauac aaauaaugga ucuaggacau augugugaug cuacaaugag uuacgaaugu      240

ccaaugcuag acgaaggagu agagccagac gauguagacu guugguguaa uacgacauca      300

acauggguag uauaugguac augucaucac aaaaaaggcg aagcaagacg aucaaggaga      360

gccgucacuc uaccaaguca cagcacaaga aaacuacaaa cuagaaguca aacaugguua      420

gaaucaagag aauauacaaa acaucuaaua aggguggaaa acuggauauu cagaaaucca      480

gguuuugcac uagcagcagc agcgauagcu ugguuauuag guaguucuac uucacaaaaa      540

guuauauacu ugguaaugau uuuauuaaua gcacccgcau acaguauaag gugcauugga      600

guaaguaaua gagauuuugu agagggaaug aguggaggaa cauggguaga uauaguauua      660

gaacauggag gcuguguaac ugugauggca caagacaaac cuacaguaga cauugaauua      720

guaacgacaa caguuucaaa uauggcagaa guaagaucuu auuguuauga agcaaguaua      780

agcgauaugg caagugacag cagaugucca acacaagggg aagcuuaucu ugacaagcaa      840

ucagacacgc aauacguuug uaaacggaca uuaguagaua ggggcugggg gaacggaugu      900

ggauuauuug gaaaaggguc cuuaguaaca ugcgcaaaau ucgccuguag uaaaaaaaug      960

acaggaaaau cuauacaacc agaaaaucuc gaauauagaa uaauguuauc aguacaugga     1020

agucaacauu cagggaugau aguaaaugau acuggccaug aaacagacga aaauagagca     1080

aaaguagaaa uaacaccuaa uagucccaga gcagaagcaa cauuaggagg auucggauca     1140

uuaggauuag acugugaacc aagaacaggc cuagauuucu cugacuuaua uuaccuaaca     1200

augaauaaua aacacuggcu aguucacaaa gagugguuuc augacauacc gcuaccuugg     1260

caugcaggug cagauacagg aacgccacau uggaauaaua aagaagcacu aguagaguuu     1320

aaagaugcuc augcaaaaag acaaacggua guaguauuag gaagccaaga aggagcagua     1380

cacacagcuc uagcaggagc ucuugaagca gaaauggaug gugcuaaagg ucgauuauca     1440

uccggacacu uaaaauguag auuaaaaaug gacaaacuaa gauuaaaagg aguaagcuac     1500

agccuaugua cagcagcauu uacauuuaca aaaauaccag cagaaacuuu acauggaaca     1560

guaacaguug aaguacaaua ugcaggaaca gaugggccau guaaaguucc ugcgcaaaug     1620

gcaguagaca ugcaaacauu aacaccagua ggaagauuga uaacagcaaa uccaguaaua     1680

acugaaucaa cugaaaacuc aaaaaugaug cuagaacuag acccaccauu cggagauagc     1740

uauauaguaa uuggaguugg cgaaaaaaaa auaacacauc auuggcacag aaguggaagu     1800

acgauuggaa aagcauuuga ggcaacagua agaggugcua aaagaauggc aguacuugga     1860

gacacagcuu gggauuuugg uagcguagga ggagcauuaa auucacuugg aaaaggaauu     1920

caucaaauau ucggagcagc cuucaaauca cuauuuggag gaaugaguug guuuucacag     1980

auauuaauug ggacacuucu aauguggcua ggauugaaua caaaaaaugg gaguauaucu     2040

uuaauguguc uagcauuagg ggguguauua auauucuugu caacagcugu gucugccgau     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  9162
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9162
gggagaaagc uuaccaugag aagaggcgca gauacaucag ucggaauugu uggucuuuug       60

uuaacuacag caauggcagc agaaguaaca agacguggaa gugcguacua cauguaccua      120

gacagaaaug augcaggaga agcaaucuca uuuccuacua cucuggguau gaacaaaugc      180

uauauacaaa uaauggaucu aggccauaug ugugaugcga caaugucaua ugagugucca      240

augcuagacg aagguguuga accugacgau guagauugcu gguguaacac aacaucaaca      300

uggguaguau auggaacaug ccaccauaag aaaggagaag cuagaagauc aagacgagca      360

guaacauuac ccagucauag uacaagaaaa uuacagacaa gaagccaaac auggcuagaa      420

ucgagagaau auacuaaaca ucuaauaaga guagaaaauu ggauauuccg aaacccagga      480

uuugccuugg cagcagcugc caucgcaugg cuuuuaggaa guucgacaag ccaaaaagua      540

auauaucuag uuaugauacu ucucauagca ccagcuuauu caauaagaug uaucgguguu      600

aguaacagag auuuuguaga agguaugucc ggagguacuu ggguugauau aguauuagaa      660

caugguggau guguuacugu aauggcacaa gauaaaccaa caguagauau agaauuagua      720

acaacuacag uaagcaacau ggcagaagua agaucuuauu guuaugaagc aucaauauca      780

gauauggcaa gugauaguag augcccaacc caaggagaag cauacuuaga uaaacaguca      840

gauacacaau auguauguaa acgaacuuua guugaucgag gguggggaaa cggauguggu      900

uuauuuggaa aaggaaguuu aguaacaugu gcuaaauuug caugcucaaa gaaaaugaca      960

ggaaaaucaa uacaaccaga aaauuuagaa uauaggauaa ugcuuagcgu acauggauca     1020

caacauuccg gaaugauugu aaaugacacg ggacaugaaa cugaugaaaa cagagcuaaa     1080

gucgaaauaa caccuaauag uccucgagca gaagcaacau uaggaggauu ugguagcuua     1140

ggacuugacu gugaaccuag aacaggacua gauuuuagug aucuauacua ccuuaccaug     1200

aauaauaaac auuggcuagu acacaaagaa ugguuucaug acauaccauu accauggcau     1260

gccggagcag acacagguac accucacugg aauaauaaag aagcccuagu agaauuuaaa     1320

gaugcacacg caaaacgaca aacaguggug gucuuaggaa gccaagaagg agcaguacau     1380

acagcauuag ccggugcucu agaagcagaa auggauggug caaaaggaag acuuagcuca     1440

ggacauuuaa aauguagguu aaaaauggau aaauuaaggu uaaaaggagu aucauauagu     1500

cuaugcacag cagcauuuac uuucacaaaa auacccgccg aaacgcuaca cggaacagua     1560

acaguagaag uacaauacgc aggaacugau gguccaugua aaguaccagc ucaaauggca     1620

guagacaugc aaacauuaac accaguaggu agguuaauaa ccgcgaaucc aguaauaacu     1680

gaaucaacag aaaauagcaa aaugauguua gaacuagacc cuccauucgg agauagcuau     1740

auaguaauag guguaggaga gaagaaaauc acacaucauu ggcauagaag uggaucaaca     1800

auaggaaaag cauuugaagc uacaguaaga ggagcaaaac guauggcagu auugggagau     1860

acugcauggg auuuuggaag uguaggggga gcucuaaauu cacuuggcaa aggaauacau     1920

caaauauuug gagcagcauu uaaauccuua uucggaggaa ugucuugguu uagccaaaua     1980

cuaauaggaa cuuuauuaau guggcuugga cuaaacacua aaaauggauc aauuucuuua     2040

augugucuag cucuaggagg aguacuuauc uuucuaagua cagcagugag ugcagacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  9163
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9163
gggagaaagc uuaccaugag aggagcagac acaaguguag guauaguugg auuauuauua       60

acaacagcaa uggcagccga aguaacaaga agagguucag cuuauuauau guauuuagac      120

agaaaugaug caggagaagc aauaucauuc ccaaccacgc uuggaaugaa uaaaugcuau      180

auacaaauaa uggauuuagg acacaugugu gaugcaacaa ugucauauga augccccaug      240

uuagaugaag guguagaacc agaugacgua gacuguuggu guaauacaac gagcacaugg      300

gucguauaug gaacuugcca ucauaagaaa ggagaagcua gacgaagcag acgagcagua      360

acgcuaccaa gucacucaac cagaaaauug caaacaagga gucaaacaug gcuagaauca      420

agagaauaua cuaaacaucu uaucagagua gaaaauugga uuuuucgaaa uccaggauuu      480

gcacuugcug cagcugcuau agcauggcua uuagguucaa gcaccucaca aaaaguaaua      540

uaucuaguua ugauacuauu aauugccccu gcauacucaa uuagauguau aggaguauca      600

aauagagacu ucguagaggg aaugucagga ggaacuuggg uagauauagu acuagaacau      660

gguggaugug uaacaguaau ggcacaagau aaaccaacug uugacauaga auuaguaaca      720

acaaccguaa guaauauggc agaaguaaga uccuacugcu augaggcaag uauaagcgau      780

auggcaucag auagucgaug uccaacacaa ggggaagcau auuuagauaa acaaucagau      840

acgcaauacg ucuguaaaag aacauuagug gacagaggau gggguaaugg uuguggacua      900

uucggaaaag guagcuuagu uacaugugca aaauuugcau guaguaagaa aaugacagga      960

aaaucaauuc aacccgaaaa cuuagaauau agaauaaugc uaucaguaca uggaagucaa     1020

cauucaggua ugauuguaaa cgauaccggc caugaaaccg augaaaauag agcuaaagua     1080

gaaauaacgc cuaauagccc aagagcggaa gcaacacugg gaggauuugg gagcuuagga     1140

uuggauugug aaccaagaac aggacuagau uucucugacu uauauuaccu aaccaugaau     1200

aauaaacauu gguuaguaca uaaagaaugg uuucaugaua uaccacuacc guggcaugca     1260

ggggcagaua ccggaacccc acacuggaau aauaaagaag ccuuaguaga auuuaaagac     1320

gcgcaugcaa aaagacaaac uguaguagua uuaggaucac aagaaggggc aguucacacc     1380

gccuuagcag gagcacuaga agcagaaaug gauggagcua aaggucgacu aucaucagga     1440

cauuuaaaau guagacuuaa aauggauaaa uuaagauuaa aaggaguuag uuacucauua     1500

ugcacugcag cauucacuuu caccaaaaua ccagcagaaa cauuacaugg cacuguaaca     1560

guagagguac aauaugccgg aacagaugga cccugcaaag uaccagcgca gauggcuguc     1620

gauaugcaaa cauuaacucc aguuggucga cucauaacug cuaaucccgu aauaacagaa     1680

ucaacagaaa auucaaaaau gaugcuugaa uuagauccuc ccuuuggaga uucauauaua     1740

guaauaggag uaggggaaaa gaaaauaacc caccauuggc auagaagugg aaguacaauu     1800

gguaaagcau uugaagcaac aguuagagga gcaaaaagaa uggcgguauu aggagauaca     1860

gcgugggacu ucggcagcgu aggaggcgcc uuaaauucuu uaggaaaagg gauacaucaa     1920

auauuuggag cagcauucaa aucauuauuc ggaggaaugu caugguuuuc acaaaucuua     1980

auaggaacuc uauuaaugug guuaggucua aauacuaaaa acggcucaau aaguuuaaug     2040

uguuuggcac uaggcggagu acuaauuuuc cuaucgacag caguauccgc agacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  9164
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9164
gggagaaagc uuaccauggg agcggauaca ucaguuggua uaguaggacu guuauuaaca       60

acagcuaugg cugcagaagu aaccagaaga ggaucagcau auuauaugua uuuagauaga      120

aaugaugcug gagaagcuau aucauuuccc accaccuuag gaaugaauaa auguuacauu      180

caaauaaugg auuugggaca caugugcgau gcaacaauga gcuaugagug ucccauguua      240

gacgaaggag uugaaccaga ugacguagau uguuggugua auacgacaag uacuugggua      300

guauacggca caugucauca caagaaagga gaagcacgcc gcucaagacg agcuguaacc      360

cuucccaguc auaguacaag gaaauugcaa acucguagcc aaacaugguu agaauccaga      420

gaauauacca aacaucucau aagaguagaa aacuggauau uuagaaaucc aggauucgca      480

uuagcugcag cagcaauagc auggcuccua ggaucaagca caucccaaaa aguuauauac      540

cuaguuauga uauuauuaau agcuccagca uauucaauua gauguauagg aguuucaaac      600

cgugacuuug uugaaggcau gucaggagga acauggguag auauaguacu agaacacgga      660

ggauguguaa cuguaauggc acaagacaaa cccacaguug auauagagcu aguuacuaca      720

acaguaagua auauggcaga aguaagaagu uauugcuacg aagcaucaau aagugauaug      780

gcaagugaca guagaugccc aacccaagga gaagcguacu uagacaaaca aagugauacc      840

caauauguuu guaaaagaac acuuguugau cgaggauggg gaaauggaug cggacucuuu      900

gguaaaggau cauuaguaac augugcaaaa uuugccugua gcaagaaaau gacaggaaaa      960

ucaauacaac cagaaaaccu agaauauaga auaauguuau cuguacaugg uucacaacau     1020

ucagguauga uugucaacga cacaggacau gaaacugaug aaaauagagc aaaagucgaa     1080

auaacuccaa auucaccacg agcagaagcc acuuuagggg guuuuggaag ucuaggauua     1140

gacugugaac caaggacagg auuagauuuu ucagaucuau auuauuuaac aaugaacaac     1200

aaacauuggc uaguacacaa agaaugguuu caugacauac cauuacccug gcacgcaggc     1260

gcugacacug gaacuccgca cuggaauaau aaagaagccc uaguugaguu caaagacgca     1320

caugcaaaaa gacaaacagu aguuguauua ggaucacaag aaggagcugu acauacagca     1380

uuggccggag cauuagaagc ugaaauggau ggagcaaaag guagacuaag cagcggccau     1440

cucaaaugca gauuaaaaau ggacaaacua agacuaaaag gcguuucaua cucacuaugu     1500

acagcagcuu uuacauuuac uaaaauaccu gcagaaacau uacaugguac aguaacaguu     1560

gaaguacaau augcaggaac agauggacca ugcaaaguac cagcacaaau ggcagucgau     1620

augcagacau uaacgccagu aggacgacua auaacagcaa auccuguaau aacagaaagc     1680

acagaaaauu caaaaaugau gcuagaacuc gacccucccu ucggggauuc auauauagua     1740

auaggaguag gcgaaaagaa aauaacacac cauuggcauc guucaggaag uacuauagga     1800

aaagcauucg aagccacagu aagaggugca aaaaggaugg caguguuagg agauacagca     1860

ugggauuuug gaucagucgg aggagcacua aauagcuuag gaaaaggaau acaucagaua     1920

uuuggagcag cauuuaaaag uuuauucggu ggaaugucuu gguucucuca aauuuuaaua     1980

ggaaccuuac uuaugugguu ggggcuaaau accaaaaaug gaucaauauc auuaaugugc     2040

uuagcucuag gagguguacu aauauuucua ucgacagcag uguccgcaga uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9165
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9165
gggagaaagc uuaccauggc ugauacauca guaggcauag uaggucuacu uuuaacaaca       60

gcaauggcag cugaaguaac aaggcgaggu agcgcuuauu acauguacuu agauagaaau      120

gaugcagggg aagcuauauc uuuuccgaca acauuaggaa ugaauaaaug cuauauucaa      180

auaauggacc ugggacauau gugugaugcg acaaugaguu augaaugccc aauguuagau      240

gaaggaguug aaccggauga cguagacugc ugguguaaua caacaaguac augggucgua      300

uaugguacau gucaucauaa aaaaggagaa gcaagaagau caagaagagc aguuacauua      360

ccuucacacu cuacaagaaa auugcaaaca cgaagccaaa cguggcuuga aucaagagag      420

uacacaaaac auuuaauccg aguagaaaau uggauuuuca gaaauccagg auucgcacua      480

gccgcagcag caauagcaug guuacucgga ucaaguacau cacaaaaagu aauuuacuua      540

guaaugauau uacuaauagc cccagcauau aguauaagau guauaggagu aaguaauaga      600

gauuuugugg aaggaaugag uggagguacg uggguagaua uuguauuaga acauggugga      660

uguguaacag uaauggcaca ggauaaacca acaguagaca uugaauuagu aacaacaacc      720

guaucaaaca uggcagaagu aagaucauac uguuaugaag caaguauauc ugauauggcg      780

agcgacucaa gauguccuac acaaggugaa gcguaucuag auaaacaauc cgauacucaa      840

uauguaugua aaagaacccu aguagacaga ggguggggca augguugugg uuuauuuggc      900

aaaggaucau ugguaacaug ugcaaaauuc gcaugcucaa aaaaaaugac uggaaaauca      960

auucagccgg aaaaccuaga auauagaaua auguuaucug uucacggaag ucaacacuca     1020

ggaaugauug uaaacgauac uggucacgaa acugaugaaa auagagcuaa aguagaaaua     1080

acaccaaaua guccaagagc agaagccacc cuugguggau ucggaagucu aggauuagau     1140

ugcgaaccaa ggaccggauu agacuucuca gauuuauauu aucuaacuau gaauaauaaa     1200

cauuggcuag uacacaaaga augguuucac gauauaccuu uaccauggca ugcaggagca     1260

gauaccggua caccacacug gaacaauaaa gaagcuuuag uagaauuuaa agaugcucac     1320

gcaaaacgac aaacgguugu aguacuagga ucacaagaag gagcagucca cacagcauua     1380

gcgggagccu uagaagcaga aauggaugga gcaaaaggaa gacuuaguuc aggacauuua     1440

aaaugccgau uaaaaaugga uaagcuuaga cuaaaaggag uaaguuacag ccugugcaca     1500

gcugcauuua cauuuacaaa aaucccagcg gaaacauuac auggaaccgu cacaguagaa     1560

guacaauacg ccggaacaga uggcccaugc aaaguaccag cucaaauggc uguagauaug     1620

caaacacuaa caccaguagg aagacuaaua acagccaacc caguaauaac cgagucuaca     1680

gaaaacagua aaaugauguu agaacucgau ccuccauuug gagauagcua uauaguaauu     1740

gguguaggag aaaaaaaaau aacacaucau uggcaucgau cagguucaac aauagguaaa     1800

gcauuugaag cuacaguacg aggagccaaa agaauggcag uauuggguga uacagcaugg     1860

gauuuuggau cugucggagg agcauuaaac ucauuaggaa aagguauaca ucaaauauuu     1920

ggugcugcau ucaaaagcuu auuuggagga augucauggu uuucccaaau ucuaauagga     1980

acauuauuaa ugugguuagg auuaaacacg aaaaaugggu caauaagucu aaugugccuu     2040

gcauuaggug gaguacuaau auuucuaagc acugcaguau cagcugauug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  9166
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9166
gggagaaagc uuaccaugga cacaagugua ggaauaguag gauuguuauu aacaacugca       60

auggcagcag aagucacucg ucgugguagc gcauacuaua uguauuuaga uagaaaugau      120

gcaggagaag caauaucuuu uccgacaaca uuaggaauga acaaauguua uauacaaaua      180

auggauuuag gucauaugug ugacgcuacg augucauaug aauguccaau guuggaugaa      240

ggaguagaac cagaugacgu cgauuguugg ugcaauacga cauccacaug gguaguuuau      300

gggacauguc aucauaagaa aggagaagcg aggagaagca ggagagcagu aaccuuacca      360

agucacagca caagaaaacu acaaacaaga agucaaacau gguuagaauc aagagaauau      420

accaaacacu uaauaagggu ggaaaauugg auauuucgaa auccaggauu ugcauuagca      480

gcagcugcaa ucgcgugguu auuaggauca ucaacaucac agaaaguaau auaccuaguu      540

augauauuau uaauagcacc agcauauagc auaagaugca uaggagugag uaauagagau      600

uuuguagaag gaaugagugg aggcaccugg guagauauag uacuagaaca uggcggaugu      660

gucaccguua uggcucaaga uaaaccaaca guagauauag aauuaguaac uacaacagua      720

ucaaauaugg cagaaguaag aaguuacugc uaugaggcuu caauaaguga cauggcuuca      780

gauucaagau guccgacaca aggagaagca uauuuagaua aacagucuga uacucaauau      840

guaugcaaaa gaacccuagu agacagagga uggggaaaug guugugguuu auucggaaaa      900

ggaagucugg uuacaugugc aaaauucgca ugcucuaaga aaaugacagg aaaaucaauc      960

caaccagaaa accuagaaua cagaauaaug uuaucaguac acggcucaca acauagugga     1020

augauaguga augauacugg acacgaaacu gaugaaaauc gagcaaaagu agaaauaaca     1080

cccaacaguc cgagagcaga agcuacauua ggaggauuug guucauuagg auuggauugc     1140

gaaccaagaa cagguuuaga cuuuagugau uuauauuauc uuaccaugaa uaacaaacau     1200

uggcuuguac auaaagaaug guuucaugac auuccucuac cauggcaugc cggagcagau     1260

acggguacuc cacacuggaa uaauaaagaa gcauugguag aauuuaaaga ugcacaugca     1320

aaaagacaaa cuguaguagu auuaggaagu caagaaggag caguucauac ugcguuagcu     1380

ggagcauuag aagccgaaau ggauggagca aagggaagau uaucaagugg acaccuuaaa     1440

ugccgacuga aaauggauaa acuaagacuu aaagguguuu cuuauucuuu auguacugcg     1500

gcuuucacau uuacgaaaau acccgcugaa acacuucaug guacuguaac aguagaagua     1560

caauaugcag gaacagacgg accauguaaa guaccagcac aaauggcagu cgauaugcaa     1620

acacuuacac caguagguag acuuaucaca gcgaauccag uuauaacuga aaguacagaa     1680

aauaguaaaa ugaugcugga auuagauccu ccuuucggag acaguuacau aguuauuggu     1740

guaggggaaa agaaaauaac acaucacugg cauagaagug gaucaacuau aggaaaagca     1800

uuugaagcaa caguccgugg agcaaaacga auggcaguac uaggcgauac ugcaugggau     1860

uuugguagcg uugguggagc acuaaauuca cuuggaaaag gaauacacca aauauuugga     1920

gcagcauuua aaucacuuuu ugguggcaug ucaugguuuu cacaaauuuu aauuggaaca     1980

uuauuaaugu ggcuaggauu aaauacuaaa aacgguagua uaucguuaau gugucuagca     2040

cuagguggag ucuuaauauu ucuuaguaca gcaguuagug cagauugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  9167
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9167
gggagaaagc uuaccaugac aagugucgga auaguaggau uauuguuaac aacggcaaug       60

gccgcagaag ugacgagaag ggggucugcu uauuauaugu aucuugauag aaaugaugca      120

ggagaagcua uaaguuuucc aacaacucua ggaaugaaua aauguuacau ucagauaaug      180

gacuuagggc auaugugcga ugcaacaaug ucauacgagu gcccaaugcu agaugaagga      240

guagaaccag augacguuga cugcuggugu aauacaacuu caacaugggu agucuacggu      300

acaugucauc acaaaaaagg agaagcaaga agaucuagaa gggcagugac auuaccuagu      360

cauagcacga ggaaacucca aacccgauca caaacauggc uagaaagcag agaauauaca      420

aagcaccuaa uaagaguaga aaauuggauu uuuagaaauc caggauucgc auuagcagcc      480

gcugcgauug caugguuacu uggaucaagu acgucgcaaa aaguuauaua cuuaguaaug      540

auauugcuaa ucgcaccagc auauucgauc agaugcauag gaguaucaaa cagagacuuc      600

guagaaggaa ugaguggagg aacaugggua gauauaguac uagaacaugg agguugugug      660

acaguaaugg cacaagauaa accaacugug gacauagaac uagugacaac aacaguauca      720

aacauggcag aaguacgauc uuacuguuau gaagcgucaa uaagcgauau ggcaagcgau      780

aguagaugcc caacacaagg agaagcauac uuagauaaac aaucagauac ucaauaugua      840

uguaaacgaa cacuaguaga uagaggaugg ggaaauggau guggauuauu uggcaaagga      900

agucugguaa cuugugcaaa auuugcuugc aguaaaaaaa ugacaggaaa aaguauacaa      960

ccagaaaacc uugaauauag gauuauguua aguguucacg ggagucaaca uagugggaug     1020

auugucaaug auacaggaca ugaaacagac gaaaauagag caaaagucga aauaacgcca     1080

aacaguccaa gagcugaagc gacauuagga ggauuuggau cauuaggacu agauugugaa     1140

ccaagaacug ggcuagacuu uucagacuua uacuaucuaa caaugaauaa uaagcacugg     1200

uuaguucaua aagaaugguu ucaugacaua ccacuaccuu ggcaugcagg agcagauacu     1260

ggaacuccgc auuggaauaa uaaagaagca uuaguagaau ucaaagaugc acaugcaaaa     1320

cgacagacag uagucguacu uggaagucaa gaaggagcgg ugcauacagc cuuagcagga     1380

gcauuagaag cagagaugga cggcgcuaaa gguagacuua gcagcggaca ucuaaaaugu     1440

agacuaaaaa uggauaaacu aagacuaaaa ggaguaucau auucauuaug uaccgcagcg     1500

uucacauuua caaaaauacc agcggaaaca uuacauggaa cuguaacagu agaaguacaa     1560

uaugcaggaa cugauggacc auguaaagua ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacacccg uaggaagacu aauaacagca aauccaguaa uaacagaaag uacagaaaau     1680

ucuaaaauga uguuggaauu agacccaccu uuuggagaca guuacauagu aauaggagua     1740

ggagaaaaaa aaauaacaca ccauuggcau aggaguggaa gcaccauagg aaaagcauuu     1800

gaagcuacug uaagaggagc gaaacgaaug gcuguucuag gagauacggc uugggacuuu     1860

ggaaguguag gaggagcguu aaauagucuc ggcaaaggaa uucaucaaau auucggagca     1920

gcauuuaaau cacuauuugg uggaauguca ugguuuaguc aaauauugau aggaacacua     1980

uuaauguggu uaggauuaaa uacuaaaaac ggcaguauau cacuaaugug cuuagcauua     2040

gguggaguuu uaauauuccu aaguacagca guaagugcag auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  9168
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9168
gggagaaagc uuaccaugac aucaguagga auuguugguu uacuauuaac aacagcaaug       60

gcagccgcag uaacacgccg aggcucggcu uacuauaugu auuuagauag aaacgaugca      120

ggagaagcaa uaucauuucc aaccacacug gguaugaaua aaugcuauau ccaaauaaug      180

gaucuaggac auaugugcga cgcaacaaug ucauaugaau guccaaugcu agaugaagga      240

gucgaaccag augauguaga uugcuggugu aauaccacaa guaccugggu aguauauggc      300

acaugucauc auaagaaagg cgaagcaaga agaaguagac gagcaguaac auuaccauca      360

cauucaacaa gaaaacuuca aacaagaagc caaacuuggc uagaaagcag agaauauaca      420

aaacauuuaa uaagagugga aaauuggaua uucagaaauc cuggcuuugc auuagcugca      480

gcggcuauag cuugguuacu uggaucuagu acuucacaga aaguaauaua ucuaguaaug      540

auacuauuaa uagcaccagc uuacaguauc cgaugcauag gaguaucgaa cagagauuuu      600

guagaaggga ugucaggugg aacaugggua gauauaguau uagaacacgg cggaugcgua      660

acaguaaugg cacaagacaa accaacagua gacauagaac uuguaacaac aacaguauca      720

aauauggcag aaguuagauc auacugcuau gaagcaucaa uaucagacau ggcuucagau      780

aguagauguc caacacaagg agaagccuac uuagauaaac aguccgauac ucaauaugua      840

uguaaaagaa cccuaguaga ccgcgguugg ggaaaugguu gcggacuauu cggaaaagga      900

agucuaguaa ccugugcaaa auuugcaugu aguaagaaaa ugaccggaaa aaguauucaa      960

ccagaaaauu uagaauauag aauaauguua aguguacaug gcucucaaca uucaggaaug     1020

auagucaaug auacagguca ugaaacagau gaaaauagag cuaaagucga aauaacacca     1080

aauucaccac gugcugaagc aacacuagga ggauuuggau cacucggauu agauugugaa     1140

ccaaggacag gcuuggauuu cucagauuua uauuaucuaa cuaugaacaa caaacauugg     1200

uuaguccaua aagaaugguu ucacgauaua ccucuaccau ggcaugcugg ugcagauaca     1260

ggaacacccc auuggaauaa uaaagaagcu cuagucgaau uuaaagaugc acacgcaaaa     1320

cgccagacag uaguuguacu aggaagccaa gaaggagcag uacacacagc ucuagcagga     1380

gcuuuagaag cagaaaugga uggugcaaaa ggaagacuaa gcucaggaca ucugaaaugc     1440

aggcuaaaaa uggauaaauu aagauuaaaa ggaguaagcu auucauuaug uacagcagca     1500

uuuacauuca caaagauacc agcggaaaca uuacacggga ccguaaccgu agaaguacaa     1560

uaugcaggua cagacggacc auguaaagua ccagcacaaa uggcaguaga caugcaaacc     1620

uuaacaccag uaggucgauu aauaacagcc aauccaguaa uaacagaauc aaccgaaaau     1680

uccaaaauga ugcuagaauu agauccaccu uucggagaua guuauauagu aauaggagua     1740

ggagaaaaga aaaucacaca ucacuggcau agaucuggcu ccacuauagg aaaagcauuu     1800

gaagcuaccg uuagaggagc aaaaagaaug gcugucuuag gagauacugc augggacuuu     1860

ggaaguguug gaggugcacu uaacagccua ggaaaaggaa uacaucaaau auucggugca     1920

gcuuuuaaaa gucucuucgg uggaaugucc ugguucucuc aaauuuuaau agguacacua     1980

cuuauguggc uuggucuaaa caccaaaaau ggaucaauau cauuaaugug ccuggcacua     2040

ggaggaguac uaauauucuu aucaacagcu guaagcgcag auugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  9169
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9169
gggagaaagc uuaccaugag aggagcagau acaaguguug guauaguagg cuuacuauua       60

acaacagcaa uggccgcugc uguaacacga agaggaagug cauauuacau guacuuagac      120

cgaaacgaug caggagaagc cauaucauuu ccaacaacgu uaggaaugaa caaaugcuac      180

auacagauaa uggaucuagg acauaugugu gacgcaacga ugucauacga auguccaaug      240

uuagaugaag gcguagaacc agaugaugua gauuguuggu gcaacacaac aaguacaugg      300

guaguauaug gcacauguca ccauaaaaaa ggggaagcca gaagaagcag aagagcggua      360

acacuaccuu cacauaguac uagaaaacuu caaacgcgcu cucaaacaug gcuugagagu      420

agagaguaua caaaacauuu aauuagagua gaaaauugga uauuucgaaa ucccgguuuu      480

gcauuagcag cagcagcaau agcauggcug cucggaucau caacuucaca aaaaguuaua      540

uauuuaguaa ugauauuacu aauagcacca gcauacagca uacgaugcau aggaguuuca      600

aacagagauu uuguagaagg aaugucugga ggaaccuggg uagacauugu gcuugagcau      660

ggaggaugcg uaacuguuau ggcacaggac aaaccaacgg uugauaucga auuaguuaca      720

acuacaguau caaauauggc ggagguuaga uccuauuguu augaggcauc uauaucagau      780

auggcaucag auucgagaug uccuacucaa ggugaagcuu aucuagacaa acaaagugac      840

acgcaauaug uuuguaaaag aacuuuaguu gacagaggau ggggaaaugg cugugguuua      900

uuuggaaaag ggaguuuagu aacaugugca aaauuugccu guucuaaaaa aaugacaggc      960

aaaucaauac aaccagaaaa uuuagaauau agaauaaugu uaucaguaca uggaagucaa     1020

cauagcggaa ugauaguuaa ugauacagga cacgaaaccg augaaaaccg ugcuaaaguu     1080

gaaauaacac caaauucacc aagagcagaa gcaacauuag gaggauucgg auccuuagga     1140

cuugacugug aaccaagaac aggacuagac uuuagugacu uauauuauuu gacaaugaau     1200

aauaaacauu gguuaguaca uaaagaaugg uuucacgaua uaccauuacc uuggcacgca     1260

ggagcagaua caggcacacc acauuggaau aacaaagaag cuuuaguaga auucaaagac     1320

gcacacgcaa aacgacaaac uguugucgua cuaggaucac aagagggugc aguacacaca     1380

gcgcuagcug gagcauuaga agcagaaaug gacggagcua aaggacgcuu aucaucaggg     1440

caccuaaaau gucgacuaaa aauggauaaa cuaagguuaa aaggaguauc cuauucccua     1500

uguacagcag cauucacauu cacaaaaaua ccagcagaaa cauuacacgg aacaguuacu     1560

guggaaguac aauaugcagg aacagaugga ccauguaaag uuccagcuca aauggccgua     1620

gacaugcaaa cguuaacacc aguaggaaga cuuauuacag cgaacccagu uauaacagaa     1680

aguacugaaa auaguaaaau gauguuggaa uuagauccuc cauuuggaga uucuuauaua     1740

guaaucggcg uaggggaaaa aaagauaaca caucauuggc acagaagcgg aucuacaauc     1800

ggaaaagcau uugaagcaac aguaagaggc gcaaaaagaa uggcaguacu aggagauacc     1860

gcaugggauu uugguagugu aggaggagca cuaaauagcc uaggaaaagg aauacaccaa     1920

auauucgggg cagcauucaa aagcuuauuu gguggaaugu caugguucag ccaaauacua     1980

auaggaacau uauuaaugug gcuuggacuc aacaccaaaa auggaucuau uagccuaaug     2040

ugcuuagcgu uaggaggagu acuaauauuc uuaucaacag caguaagugc agauugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  9170
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9170
gggagaaagc uuaccaugcg aggauuauca ucaaggaaac gaagaagcca ugacgucuua       60

acuguacaau ucuuaauacu aggaaugcua uuaaugacag gcgguguaac aagaagagga      120

agugcauacu acauguaucu ugacagaaac gaugcgggug aagccauaag cuuuccaaca      180

accuuaggaa ugaauaaaug uuacauacaa auuauggacu uagggcauau gugcgaugca      240

acaaugaguu augaaugucc uaugcuagau gagggaguag aaccugacga uguagacugu      300

uggugcaaca caacaucaac uugggucguu uaugggacuu gucaccauaa aaaaggugag      360

gcaagaagaa guagaagagc aguaacauua ccuucacauu ccacaagaaa auugcaaaca      420

cgaucacaaa caugguuaga aaguagagaa uacacaaaac accuuauaag agucgaaaau      480

uggauauuuc gaaauccagg cuuugccuug gcugcugcag caauugccug gcuauuaggu      540

aguucuacua gucaaaaagu gauauauuua gugaugauuu uauugauagc accggcauau      600

agcauaagau guauuggcgu aaguaauaga gauuuuguug aagguaugag uggugguacc      660

uggguagaua uaguccuaga acauggagga uguguaacag uaauggcaca agauaaacca      720

acaguagaua ucgaacuagu aacuacaacu gucucaaaca uggcagaggu uagaaguuau      780

uguuacgaag caaguauaag ugauauggca agcgauucuc gauguccaac acaaggugaa      840

gcuuacuuag auaaacaauc agauacacaa uauguaugua aaagaacacu aguugacaga      900

ggauggggua auggaugugg acuauuugga aaaggauccu uagucacuug cgcuaaauuu      960

gccuguagca aaaaaaugac ggguaaauca auacaacccg aaaauuuaga auaccgaauu     1020

auguugagug uucauggauc ucaacauuca gguaugauug uaaaugauac aggacaugaa     1080

acagaugaaa accgagcaaa aguagaaauc acuccaaaca gcccuagagc agaggcaacc     1140

cuuggaggau ucggaaguuu aggccuagau ugugaaccaa gaacaggauu agauuuuagu     1200

gacuuauacu accuaacaau gaauaauaaa cauugguuag uacauaaaga augguuucau     1260

gauauacccu uaccuuggca ugcuggagca gacacaggaa cgccacauug gaauaacaaa     1320

gaagcacuag uugaauuuaa agaugcacau gcaaaaagac aaacaguagu aguauuagga     1380

agucaagagg gagcagugca cacagcuuua gcaggcgcac uagaagcuga aauggacggu     1440

gcaaaaggua gacuaucaag uggacacuua aaauguagac uaaaaaugga caaauuaaga     1500

uuaaaagggg ugucauauuc uuuauguaca gcugcauuua cauucacuaa aauuccagca     1560

gaaacacuac auggaacagu aacaguggaa guacaauaug caggaacaga uggaccaugc     1620

aaagugccug cacaaauggc aguagauaug caaaccuuaa ccccaguagg aagauuaaua     1680

acggcaaacc cgguaauaac cgaaucaacu gaaaacagua aaaugauguu ggaacuagau     1740

ccaccauuug gagauucaua uauaguuaua ggagugggag aaaaaaaaau aacccaucau     1800

uggcauagaa guggaagcac aauaggaaag gcauucgaag cuacagucag aggagcaaaa     1860

agaauggcug uucuuggaga uacagcaugg gauuuuggua guguuggugg agcuuuaaau     1920

agccuuggaa aaggaauuca ucaaauauuu ggagcagcau ucaaaagcuu auuuggagga     1980

augucuuggu uuucacaaau auugauagga acacuuuuaa ugugguuagg auugaacaca     2040

aagaacggaa guauaucuuu aauguguuua gcauuaggug guguucuaau uuuucuauca     2100

accgcaguaa gugcagauug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9171
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9171
gggagaaagc uuaccaugag aggauugagu aguagaaaac gacgaaguca cgauguucua       60

acaguacaau uucuaauuuu agguaugcua uugaugacag gagcaguaac aagacgagga      120

ucagcauauu auauguauuu agauagaaau gaugcaggag aagcaauaag uuuuccaacu      180

acuuuaggaa ugaacaaaug uuauauucaa auaauggauu uaggccauau gugugaugcu      240

acaaugaguu augaaugccc aauguuagac gaaggaguag aaccagacga cguagauugu      300

ugguguaaua caacaucaac auggguagug uauggcacau gucaucacaa gaaaggagaa      360

gcgagaagaa guagacgagc aguuacacua ccaagucaua gcacgagaaa auuacaaaca      420

agaucacaga ccugguuaga aucaagagaa uacacuaaac accugauaag aguagaaaau      480

uggauuuuua gaaaucccgg auuugcacua gcagcugcag cuauagcuug gcuauuaggu      540

aguuccacua gccaaaaagu aauuuaccua guaaugauau uacuaauagc accagcauau      600

aguauaagau gcauaggagu gucaaauaga gauuuuguag aaggaaugag cggugguaca      660

uggguggaua uagucuuaga acauggagga uguguaaccg uaauggcaca agacaaacca      720

acaguugaua ucgaacuugu aacaacaacg guaucaaaua uggcugaagu aagaucauac      780

uguuaugaag caaguauauc agauauggca agugacucaa gaugcccaac acaaggagaa      840

gcauaucuag auaaacaauc agacacacaa uaugugugca aaagaacauu aguagacaga      900

ggauggggaa auggaugugg acuauucgga aaagggagcu uaguaacaug cgcuaaauuc      960

gcgugcagua agaaaaugac aggaaaaagu auacaaccag aaaauuuaga auauagaaua     1020

augcuaucag uacacggauc gcaacauuca gguaugauag uaaacgauac aggacaugaa     1080

acagaugaaa accgagcuaa aguggaaaua acgccaaauu cacccagagc cgaagcaaca     1140

uuaggggguu ucggaaguuu gggauuggau ugcgaaccua gaacgggguu agauuuuuca     1200

gauuuauauu accuaacaau gaauaauaaa cauuggcucg uacacaaaga augguuucau     1260

gauauaccuu uaccauggca ugcaggagcc gauacuggua cuccacauug gaauaauaaa     1320

gaagcccuag uagaauuuaa ggaugcucac gcaaaaagac aaacaguugu gguucuagga     1380

agucaagaag gagcgguaca uacagcauua gcaggcgcau uagaagcuga aauggaugga     1440

gcgaaagguc gacugaguuc aggacaucua aaauguagau uaaagaugga uaaacuaaga     1500

cuaaaaggag uaucauauuc auuauguacu gcagcuuuua cguuuaccaa gauuccagca     1560

gaaacacuac auggaacugu uacaguagaa guucaauaug cuggaacaga cggaccaugc     1620

aaaguacccg cacagauggc uguagacaug cagacauuaa ccccaguagg acgacuaaua     1680

acggcuaacc cagucaucac ugaaucaacu gaaaauucga aaaugaugcu agaacuagac     1740

ccaccuuuug gagacaguua uauaguuauu ggaguuggag aaaagaaaau aacacaucac     1800

uggcauagaa gcggaaguac aauagguaaa gcauuugaag caacaguacg cggugcaaaa     1860

agaauggcag uuuuaggaga uacugcaugg gauuucggau cuguaggugg agcacuaaac     1920

ucucuuggaa aagguauuca ucaaauauuu ggagccgcuu uuaaaaguuu guuuggagga     1980

augagcuggu uuucacagau auuaaucgga accuuacuaa uguggcuagg ucuaaauacc     2040

aaaaauggau caauaaguuu aauguguuua gcacuuggag gaguauuaau auuccugagc     2100

acagcaguaa gugcggacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9172
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9172
gggagaaagc uuaccaugcg aauaauaaac gccagaaaag aaaaaaagag aagaggggca       60

gauacaucug uggguaucgu aggauuauua cuuacaaccg cuauggcggc ggaaguaaca      120

agaagaggau cagccuacua uauguaccua gacagaaaug augcugguga agcaauaucu      180

uucccuacaa cuuuaggaau gaauaaaugu uacauccaaa uaauggacuu aggacauaug      240

ugcgaugcaa cuaugucgua ugaauguccu auguuagaug aaggaguaga accggaugac      300

guugauugcu gguguaacac aacaagcacc uggguaguau acgggacaug ccaucauaaa      360

aaaggggaag caagacgaag ccgaagagcu guuacuuuac caagucauuc aacgagaaaa      420

cuucaaacga gaucucaaac auggcuugaa uccagagaau auacaaaaca uuuaaucagg      480

guagaaaacu ggauuuuuag aaauccagga uucgcauuag cagccgcggc gauagcaugg      540

cugcuagggu caagcacauc ucaaaaagua auauaucuag uaaugauacu auuaauagcc      600

ccagcauauu caauaagaug uauaggagua aguaacagag auuuuguaga aggaaugucu      660

gguggaaccu ggguagauau aguauuagaa cauggaggau guguaacugu cauggcacaa      720

gauaaaccca ccguagacau agaacuggua acaacaacag uaucaaauau ggcugaagua      780

cguaguuauu gcuaugaagc aucaauauca gauauggccu cagauucacg auguccuacc      840

caaggagaag ccuacuuaga uaaacaaucc gacacacaau acguguguaa aagaacauua      900

gucgauagag gcuggggaaa uggcugugga uuauuuggaa aaggaucacu aguaacaugu      960

gcaaaauucg cgugcucaaa aaaaaugaca ggaaaaucca uucaaccuga aaacuuagaa     1020

uauagaauua uguuaagugu acacggguca caacauagcg gaaugauagu aaacgacaca     1080

ggacaugaaa cagacgagaa cagagccaaa guagaaauaa caccaaauag cccaagagcu     1140

gaagcaacac uagguggauu cggaaguuua ggauuagacu gugagccacg aacugguuua     1200

gacuucagcg aucuguacua uuuaacaaug aacaauaaac acuggcuagu acauaaagaa     1260

ugguuccaug auauaccauu gccauggcau gcaggagcag auacaggaac accacauugg     1320

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaaagaca gacgguagua     1380

guuuuaggau cacaagaagg agcuguccau acagcucucg cgggcgcacu ugaagcugaa     1440

auggacggag cgaaaggcag acuaucaucc ggucacuuaa aauguagacu aaaaauggau     1500

aaacuaagac uuaaaggagu aucauauuca uuauguacug cugcauuuac auuuacuaaa     1560

auaccagcag aaacacuuca uggaacggua acuguagaag uacaauaugc ugggacagau     1620

ggaccaugca aaguaccagc acaaauggca guugacaugc aaacauugac accaguugga     1680

agauuaauua cagcaaaucc uguaauaaca gaaagcacag agaauucaaa aaugauguua     1740

gaauuagauc caccguucgg agauagcuau auaguaauug gaguaggaga aaaaaaaaua     1800

acacaucauu ggcacagauc aggaucaacu auaggcaaag cuuuugaagc aacaguuagg     1860

ggcgcaaaaa ggauggcagu auuaggagau acagcauggg acuuuggaag cguaggaggu     1920

gccuuaaaua gucuuggaaa aggaauacau caaauuuuug gagcagcauu uaaaagucua     1980

uuugguggaa ugaguugguu uagucaaauu cuaauaggaa cucuauuaau guggcuagga     2040

cuaaauacaa aaaacggguc aauaucauug augugucuug cauuaggagg agugcuaaua     2100

uucuuaagua cagccguaag cgcuugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  9173
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9173
gggagaaagc uuaccaugau aaacgcaaga aaggaaaaaa aaagaagagg ugccgauaca       60

ucaguaggaa uuguugguuu guuguuaaca acagcaaugg cagcagaagu uacgagaaga      120

ggaucagcau acuacaugua ucuugaccga aacgaugcag gcgaagcaau aagcuuuccc      180

acaacauuag gaaugaauaa auguuauauu cagauaaugg auuuaggaca uaugugcgau      240

gcuacgauga guuaugaaug ucccaugcua gaugaaggag uugaaccaga ugauguggau      300

uguuggugua auacaacauc uacgugggua guauacggaa caugccauca uaaaaaaggc      360

gaagcaagaa gaucaagacg agcgguaaca uuaccuucac auucaacuag aaaacuccaa      420

acgagaagcc agacauggcu ggaaucaaga gaauauacaa aacaucuuau uagaguagaa      480

aacuggauau uucgaaaucc agguuucgcu uuagcagcag cagcaauagc auggcuacua      540

ggaucuucua caucacaaaa aguaauauau uuaguaauga uuuuguuaau agcaccagca      600

uacaguauaa gguguauagg uguaaguaau agagacuuug uagaaggaau gagcggagga      660

acguggguag acauaguauu agaacaugga ggauguguua caguaauggc acaggauaaa      720

ccuaccguag acauagaauu ggucacaacu acagucagca acauggcuga aguacgaagu      780

uauugcuaug aagcuucaau uagugauaug gcaucagaua gcaggugucc aacacaaggu      840

gaagcauauu uagauaaaca aagcgauaca caauauguau guaaaagaac auuaguagac      900

agaggauggg ggaaugguug uggacuauuu gguaaaggaa gcuuaguaac augcgcuaaa      960

uuugcaugcu caaaaaaaau gacuggcaaa agcauacaac cagaaaauuu agaguaucga     1020

auaauguuau cagugcacgg aagucaacac ucaggaauga ucgugaauga cacaggccau     1080

gaaacggaug aaaacagagc aaaaguagaa auaacuccaa acucaccaag agcagaagca     1140

acacuaggag gauuuggauc auuaggacua gacugcgaac cuagaacagg acuagauuuc     1200

ucagauuuau acuaucuuac aaugaauaac aaacauuggu uaguccauaa agaaugguuu     1260

caugauauuc cauuaccaug gcaugcagga gcugauacag gaacgcccca uuggaauaau     1320

aaggaagcuu uaguagaauu uaaagaugca cacgcaaaaa gacaaacagu aguuguauua     1380

gggagucaag agggugcagu acacacagca uuagcuggug cgcuagaagc agaaauggac     1440

ggggcaaaag gaagacuuag uucuggacau uuaaaaugca gacuaaaaau ggauaaauua     1500

agauuaaaag gcguaaguua uaguuuaugc acugcugcau ucacauuuac aaaaauacca     1560

gcagaaacau uacauggaac gguaacagua gaaguacaau augcaggaac ugauggacca     1620

ugcaaagugc ccgcacaaau ggcaguggau augcaaacgc ucacaccagu aggaagauua     1680

auaacagcua auccagucau aacagaauca acugaaaauu caaagaugau guuagaauua     1740

gacccaccuu uuggagauuc auacauugua auaggaguag gagaaaaaaa aauaacacau     1800

cauuggcaua gaaguggauc aacaauagga aaagcauuug aagcaacagu acgaggggcc     1860

aaaagaaugg caguacuagg agauacagca ugggacuuug gaucgguagg gggagcucua     1920

aauuccuuag gaaaaggcau acaucaaaua uuuggagcgg cguucaaauc uuuauuuggc     1980

gguaugucuu gguucucuca aauuuuaaua ggaacccuuc uaaugugguu agguuuaaau     2040

acuaaaaaug gaucuaucag cuuaaugugc uuagcccuag gagguguauu aauuuuuuua     2100

ucaacagcag uuucagcuug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9174
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9174
gggagaaagc uuaccaugau aauaaaugcu aggaaagaaa aaaaaagacg gggagcagau       60

acaaguguag gaauaguagg auuacuacua acaaccgcca uggcagcaga agucacaaga      120

agagguucag cauacuauau guacuuagac aggaacgaug caggagaagc cauuaguuuu      180

ccgacaacac uagguaugaa caaauguuau auacaaauaa uggauuuagg acauaugugc      240

gaugcaacaa ugucauauga augcccaaug uuagacgaag gaguagaacc agaugauguc      300

gauuguuggu gcaauacaac aucaacaugg guaguauacg guacguguca ucacaaaaaa      360

ggcgaagcac guagaagucg aagagcagua acucuaccaa gccauaguac aagaaaacua      420

caaacucgau cacaaacgug guuagaaagc agggaauaua cuaaacacuu aauaagagua      480

gaaaacugga uauuucgaaa uccagguuuu gcuuuagcug cagcagcaau agcauggcua      540

uuagggucau ccacgucaca aaaaguaaua uauuuaguaa ugauauuacu aauagcacca      600

gcguacucca uaagauguau aggaguuagc aauagagacu ucguugaagg aaugucuggu      660

ggaacauggg uugauauagu ccuugaacac ggaggaugug uaacggucau ggcacaagau      720

aaaccaacug uugauauaga acuaguaacc acaacaguaa gcaauauggc agaagucaga      780

ucauauuguu acgaagccag uauaagcgau auggcaucag auucaagaug uccaacucag      840

ggugaagcau auuuggauaa acaaucagau acacaauaug ucugcaaacg caccuuagua      900

gauagagggu gggguaaugg uuguggacua uuuggaaaag gaucacuagu aacaugcgca      960

aaauucgcau guucaaaaaa aaugacaggg aaauccauac aaccagaaaa uuuagaauac     1020

agaauaaugc uaucagucca uggaucacaa cauaguggua ugauuguuaa ugauacaggu     1080

cacgaaacag augaaaauag agcaaaagua gaaauaacac caaauagucc aagagcagaa     1140

gcaacauugg gaggauuugg aucucuaggu cuagauugug aaccuagaac aggacuggac     1200

uuuagugacc uuuauuacuu aacaaugaac aauaaacauu ggcuaguaca uaaagagugg     1260

uuccaugaua uaccacuacc auggcaugcc ggagcagaua caggaacucc ccacuggaau     1320

aauaaagaag cucuaguaga auuuaaagac gcacaugcca aaagacaaac aguagucgua     1380

uuaggauccc aagaaggagc cguacauaca gcacuagccg gagcauuaga agcagaaaug     1440

gauggagcaa aaggacgacu gagcucaggu caucuaaaau guagauuaaa aauggacaaa     1500

uuaagauuaa aagggguguc auacagucua uguacagccg cauuuacuuu cacaaaaaua     1560

ccagcugaaa cauuacacgg cacagugacc guugaaguac aauaugcagg aacagauggu     1620

ccaugcaaag uacccgcaca aauggcaguc gauaugcaaa cauuaacacc aguaggaaga     1680

uuaaucacag caaauccagu aauaacagaa aguaccgaaa auucaaaaau gauguuggaa     1740

uuagauccac cauuuggaga uaguuauaua guaauaggag uuggagaaaa aaaaaucacc     1800

caccacuggc acagaagugg caguacaaua gguaaagcgu ucgaagcaac ugucagagga     1860

gcaaaaagaa uggccguacu uggagacaca gcaugggauu uuggaucagu uggcggcgcu     1920

cugaacuccc uaggaaaagg aauccaucaa auauuuggag cagcauuuaa aaguuuauuu     1980

ggagguauga guugguucuc acaaauacua auuggaacac uucuaaugug gcuaggacua     2040

aauacaaaga acggcaguau aucauugaug uguuuagcgc uaggaggcgu acuaaucuuu     2100

cuaagcacag cgguuucagc uugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9175
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9175
gggagaaagc uuaccaugaa ugcaagaaaa gaaaagaaaa gaagaggugc cgauacaucc       60

guagguauag uuggcuuauu auuaacaaca gccauggcag cugaagugac uagaagaggu      120

ucagcauauu acauguaucu agauagaaau gacgcaggag aagcaauaag uuuucccacu      180

acauuaggaa ugaauaaaug uuauauacaa auaauggauc uuggacacau gugcgaugcu      240

acaaugaguu augaaugucc aauguuagau gaaggagugg aaccagauga uguagauugu      300

ugguguaaca cgacaucaac uuggguaguu uauggaacau gucaucauaa gaaaggagaa      360

gcaagacgau caagacgagc aguaacacuc ccaagccacu cuacaagaaa auuacagaca      420

agaucacaaa caugguuaga aucccgagaa uacacaaaac accuaauuag aguagagaac      480

uggauuuuua ggaauccagg auuugcauua gcggccgcug caauagcaug gcuacuagga      540

ucaaguacaa gucaaaaagu caucuauuua guaaugauau uauuaauagc cccugcauac      600

ucaauaagau guauuggcgu aaguaauaga gacuuuguag aagggaugag uggaggaacu      660

uggguagaua uaguauuaga gcauggagga uguguaacag uaauggcaca agacaaacca      720

acuguagaua uugaguuagu aacaacaacu gugaguaaca uggcagaagu aagauccuau      780

ugcuacgaag cauccauuuc agauauggcu ucagauagua gauguccaac ucaaggagaa      840

gccuaucuag acaaacaaag ugacacacaa uauguuugua aaagaacauu aguagauaga      900

ggauggggua auggaugugg cuuauucgga aaagguucau uaguuacaug cgcaaaauuu      960

gcuuguucaa agaaaaugac cggcaaauca auucaacccg aaaauuuaga auauagaaua     1020

augcucagug uacacggaag ccaacauuca gguaugauag uaaacgauac ugggcaugaa     1080

acagacgaaa auagagcuaa aguagaaauu acaccaaauu ccccaagagc agaagcuaca     1140

uugggaggau uugggagucu aggacuugau ugugaaccaa gaacaggucu agacuucagu     1200

gaucuguacu aucuuacaau gaauaacaaa cauuggcuug uccauaaaga augguuucac     1260

gacauaccac uaccauggca ugcaggagca gauacuggaa cgccacacug gaauaacaaa     1320

gaagcauuag uugaauuuaa agaugcacau gcgaaaagac aaacaguagu aguuuuaggu     1380

agccaagaag gagcuguaca uacagcauua gcaggagcau uagaagcaga aauggaugga     1440

gcgaaaggua gauuaucauc aggacaucua aaaugucgac uaaaaaugga uaaauuaaga     1500

cucaaaggag uuucauacuc auuauguaca gcagccuuua cuuucaccaa aauaccagca     1560

gaaacacugc auggaacugu aacaguugaa guacaauacg caggaacaga uggaccaugu     1620

aaaguaccag cgcagauggc aguagauaug caaacacuca caccuguagg uagacuaauc     1680

acagcaaauc cgguaauaac agaaaguaca gaaaauucua aaaugaugcu agaacuagau     1740

ccgccauucg gagacagcua cauaguaaua ggaguuggug agaagaaaau aacacaccau     1800

uggcacagau caggaaguac aauaggaaaa gcauuugaag caaccguaag aggagccaaa     1860

aggauggcag ucuuaggaga uacagcaugg gauuuuggaa guguaggggg ugccuuaaau     1920

uccuugggua aagguauaca ucaaauuuuu ggagcagcau uuaaaucauu auuuggcggc     1980

auguccuggu ucucacaaau acugauagga acucuauuaa uguggcuugg cuuaaauacg     2040

aaaaauggaa guauaucauu gaugugccua gcauuaggcg gaguucuaau auuuuuaagu     2100

acagcaguau cagcaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9176
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9176
gggagaaagc uuaccauggc aagaaaagaa aagaaaagaa gaggagcgga cacaagugug       60

ggaauaguag gacuauuauu aacaacagcu auggcagcag agguaacgag gagaggauca      120

gcauauuaua uguaucuaga ccgaaaugau gcaggagaag cuauuucauu cccaacgacu      180

cuaggaauga acaaaugcua cauacaaaua auggacuuag gacauaugug ugacgccaca      240

augaguuaug aaugcccaau gcuagaugaa ggcguagaac cagaugacgu agauuguugg      300

ugcaauacaa ccucgaccug gguagucuac ggaacauguc accauaagaa aggagaagca      360

agaaggucaa gacgagccgu cacuuuaccu agccauucaa cacgaaaacu acaaacaaga      420

ucacaaacau ggcuagaaag uagagaauau accaaacauu uaauaagagu agaaaauugg      480

auauucagaa acccaggauu cgcauuagcu gcagcagcua uagcuuggcu auuaggcagc      540

ucaacaaguc aaaaagugau auaucuggua augauacuac uuauagcucc ugcauacuca      600

auuagaugua uaggcguauc aaauagagau uuuguugaag gaaugagcgg aggaacaugg      660

guagacauag uauuagaaca cgguggaugu gugacaguua uggcacaaga uaaaccaaca      720

guagauauug agcuaguuac uacaacagua ucaaacaugg ccgaaguaag aaguuacugc      780

uacgaagcau caauaaguga uauggcauca gauagcagau guccaaccca aggugaagca      840

uauuuagaua aacaaucaga uacucaauau guuuguaaaa gaaccuuagu agauagagga      900

uggggaaaug gauguggguu auuuggaaaa ggaaguuuag uaacaugcgc uaaauuugcc      960

uguucgaaga aaaugacagg aaaaucuauu caaccagaaa aucuagaaua ccgaauaaug     1020

cuuagcgugc auggaaguca acacagcgga augauaguca acgauacugg acaugaaacg     1080

gaugaaaaca gagcaaaagu ugaaauaaca ccgaauaguc ccagagcaga ggcaacauua     1140

ggaggauuug guuccuuagg acuagacugc gagccaagaa caggccuaga uuucagcgau     1200

uuauauuauc uaacuaugaa caauaaacac ugguuggucc auaaagaaug guuucaugau     1260

auacccuuac cuuggcaugc uggugcagau acaggaacac cacauuggaa uaauaaagaa     1320

gcacuuguag aauuuaaaga ugcacaugca aaaagacaaa caguaguagu cuuaggaucu     1380

caagagggag caguacacac ggcacuugcu ggggcauuag aagcagaaau ggauggagcu     1440

aaaggucgau uaaguagugg acacuuaaaa ugccgauuaa aaauggacaa auuaagauua     1500

aaaggaguau cauauucacu augcacagcu gcuuucacau uuacaaaaau accagcugaa     1560

acacuacaug gaacaguaac aguagaagua caauacgcag gaacagaugg accguguaaa     1620

guaccagcac aaauggcagu ggauaugcaa acacuuacgc caguaggaag auuaauaaca     1680

gcuaaucccg uaauuacuga aaguacagaa aauucuaaaa ugaugcuaga acuagaccca     1740

ccuuucggug auaguuauau aguaauagga guaggagaaa agaaaaucac acaucauugg     1800

caucgaucag gaaguacaau aggaaaagca uuugaagcua caguaagagg cgcuaaacga     1860

auggcaguuc ugggagauac ugcaugggau uuuggaagug uaggaggagc acuaaacuca     1920

uuaggaaaag gcauccacca aauauuugga gcugcauuua aaagucuauu cggagguaug     1980

ucaugguuca gucaaauauu aauaggaacg uuauugaugu gguuaggcuu aaauacuaaa     2040

aacgguucaa uuucacuaau gugucuagca cuaggaggag uauuaauauu cuuaaguacg     2100

gcaguaagug caugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  9177
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9177
gggagaaagc uuaccaugcg gaaagaaaaa aaaagaagag gugcugauac aaguguagga       60

aucguaggac uauuauuaac aacagccaug gcagccgaag uaacaaggag aggcuccgca      120

uauuauaugu aucuagaucg aaacgaugcu ggugaagcaa uaucuuuucc aacaacacua      180

ggaaugaaua aauguuauau acaaauuaug gaucuagggc auaugugcga ugcaacuaug      240

aguuaugaau gcccaauguu agacgagggu guagaacccg augauguuga uuguuggugu      300

aauacaacaa gcacaugggu aguauaugga acaugccacc acaaaaaagg ugaggcaaga      360

agaagccgaa gagcuguaac auuaccauca cauagcacua gaaaauuaca aacgagaagc      420

caaacauggu uagaaucaag agaguacacu aagcaucuaa uuagaguaga aaacuggaua      480

uuuagaaauc caggauucgc acuugcagca gcggcaauag cauggcuauu aggaucauca      540

acaagucaaa aaguaauaua uuuaguuaug auacuuuuaa uagcacccgc auauagcauc      600

agaugcauug gaguaucaaa uagagacuuu gucgaaggua ugucuggagg uaccugggua      660

gauauaguau uagaacaugg uggaugugua acgguaaugg cacaagacaa accaacugug      720

gauauagaac uaguaacuac aacaguaagu aauauggcag agguaagauc uuacugcuau      780

gaagcaucaa uaucagacau ggcaagcgac ucaagaugcc cgacacaagg agaagccuau      840

uuagauaaac aaucggacac gcaauauguu uguaaacgca cuuuaguaga uagaggaugg      900

gguaauggau gugguuuauu uggaaaagga agcuuaguua caugugcuaa auucgcaugu      960

ucaaaaaaaa ugacaggaaa aucaauacaa cccgaaaacu uggaauaucg aauaauguua     1020

agcguacaug guucacaaca cucagguaug auugucaaug acacagguca ugaaacagac     1080

gaaaacagag caaaaguaga aauaacgcca aacucaccua gagcugaagc aacacuaggu     1140

gguuuuggau cucugggacu agauugcgaa ccaagaacag gacucgauuu cagugauuua     1200

uauuacuuaa cuaugaauaa uaaacacugg cuaguccaca aagaaugguu ucaugauaua     1260

ccacugccau ggcacgcagg ugcagauacc gguacacccc auuggaauaa caaagaagca     1320

cuaguggaau uuaaagaugc acaugccaaa cgucaaacag ugguaguuuu aggaagucaa     1380

gaaggagcag uccauacggc ucuagcaggu gcacuagagg cagaaaugga uggggcuaaa     1440

ggaagauuau caaguggaca uuuaaagugu agacuaaaaa uggauaaauu gagauuaaaa     1500

gggguaaguu acucauuaug cacagcugca uuuacauuca caaaaauucc agcagaaaca     1560

cuacauggca caguaacagu agaaguccaa uacgcaggaa ccgauggucc guguaaggua     1620

ccugcacaaa uggcaguaga caugcaaaca uuaacaccag ucggccgacu aauaaccgca     1680

aauccaguaa uuacagaaag uacagaaaau aguaaaauga uguuagaauu agauccacca     1740

uuuggagauu ccuacauugu uauaggagua ggugagaaaa aaauaacgca ucauuggcac     1800

agaaguggaa guacuauagg caaagccuuu gaagcuacag uaagaggggc gaaaagaaug     1860

gcaguauuag gagauacagc uugggauuuu ggaagugucg gcggcgcacu uaauaguuua     1920

ggcaaaggua uucaccaaau auuuggagcg gcauucaaau cacuauucgg aggaaugucu     1980

ugguuuucuc aaauauuaau aggaacauua cuaauguggu uaggacuaaa uaccaaaaau     2040

ggcuccauau cacucaugug uuuagcauua ggagggguac uaauauucuu aagcacagca     2100

guaucagcau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9178
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9178
gggagaaagc uuaccaugaa agaaaaaaaa agaagaggag cagauacaag uguagguaua       60

guaggauugu uacuaacaac agcuauggca gcagaaguaa caagaagagg aagugcauau      120

uauauguacc uagauagaaa ugaugcaggu gaagcaauaa guuuuccaac aacauuggga      180

augaauaaau guuauauaca aauaauggau uuaggacaua ugugugacgc uaccauguca      240

uacgaauguc caaugcuaga ugaaggagua gaaccagaug auguagacug uugguguaac      300

acuacuucaa cauggguagu auauggaaca ugccaucaua aaaaaggaga agcaagaaga      360

ucaagaagag caguaacacu accaucacau ucuacaagaa aauuacaaac acgaagccaa      420

acauggcuug aaucacgaga auauacaaaa caucuaauac guguagaaaa cuggauauuu      480

agaaacccgg gauucgcuuu agcagccgcu gcaauagcau ggcugcuagg uucaucaacc      540

ucacaaaaag ucauauaucu aguuaugaua cuacuaauag cucccgcaua cucaaucaga      600

uguauaggag uaagcaauag agauuuuguu gaaggaaugu caggagguac auggguagau      660

auaguguuag aacauggagg auguguaaca guaauggcgc aagauaaacc aacaguagau      720

auagaauuag uaacaacuac aguaaguaac auggcugagg uuagaaguua cuguuacgag      780

gcgucuauaa gcgauauggc aucagacagu agauguccaa cccaagguga agcauauuua      840

gauaagcaga gugacacaca auauguaugc aaaagaacgu uaguugaucg aggaugggga      900

aauggaugug gauuauuugg uaaaggaagu cuaguaacau gugccaaauu ugcauguagu      960

aaaaaaauga caggaaaaag cauacaaccu gaaaaucuag aauauaggau aauguuaagu     1020

guacauggaa gccaacacuc uggcaugauu guaaaugaca caggucauga aacggacgag     1080

aacagagcga aaguagaaau aaccccgaau agccccagag cugaagcaac auuaggggga     1140

uuugguaguc uagguuuaga uugugaacca agaacagggu uagauuuuag ugauuuauau     1200

uacuugacaa ugaacaacaa acauuggcua guacacaaag aaugguuuca cgauauacca     1260

cuacccuggc augcuggagc ggacacaggu acaccccacu ggaauaauaa agaagcucua     1320

gucgaauuua aagaugcaca ugcaaaaaga caaacaguag uaguuuuagg aucacaagaa     1380

ggagcaguac auacggcacu agcaggagcg uuagaagcag aaauggaugg ggcuaaaggg     1440

cgauuaucau caggacacuu aaaaugucga uuaaaaaugg auaaacuaag auuaaaagga     1500

guaucauaua guuuaugcac agcagcauuu acuuuuacaa aaauaccagc cgaaacacuu     1560

caugguacug uaacagugga aguccaguau gcuggaaccg auggaccaug uaaagugcca     1620

gcgcaaaugg caguagauau gcaaacauua accccgguag gcagacuuau aacugccaac     1680

ccuguaauaa cagaaucaac ggaaaacuca aaaaugaugc uugaauuaga uccgccauuu     1740

ggagacagcu acauuguaau agguguagga gaaaaaaaaa uaacacacca cuggcauagg     1800

ucaggaucga ccauugguaa agcguuugaa gcaacaguac gaggagcuaa aagaauggca     1860

guacuuggag acacagcuug ggauuuuggu ucuguaggag gugcauuaaa cagccuagga     1920

aaaggaauac aucaaauauu uggagcagcu uuuaaaaguc uauuuggugg aaugaguugg     1980

uuuagccaaa uauuaauugg aacguuauua augugguuag gguuaaauac aaagaacggu     2040

aguauuaguc uuaugugcuu agcuuuagga ggaguacuaa uauuuuuaag cacagccgua     2100

ucagcaugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  9179
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9179
gggagaaagc uuaccaugga aaagaaaaga agaggggcgg auacauccgu aggaauaguu       60

ggacuauuau uaacaacugc aauggccgca gaaguaacac guagaggauc agcuuauuau      120

auguaucuag auagaaacga ugcaggagaa gcuauaaguu uuccaacaac acuaggaaug      180

aauaaauguu auauacaaau aauggaccuc ggccacaugu gugacgcuac aaugucuuau      240

gaauguccca uguuagauga aggagucgaa ccagaugacg uagauugcug guguaauaca      300

acaucuacuu ggguaguaua ugguacaugu caucacaaga aaggugaagc aagaagauca      360

cgaagggcag uaacuuuacc aucucacagc acuagaaaau uacaaacuag aagccaaaca      420

ugguuagaau cuagagaaua uacaaaacau uuaauacgag uagaaaauug gauauuuaga      480

aacccugguu uugccuuggc agccgccgcu auagcauggu uacuaggauc aucaacaagc      540

caaaaaguaa uauaccuagu uaugauacua cuaauugcac cagcauauuc aauuaggugu      600

auaggaguua gcaauagaga uuuuguagaa gguaugucag guggaacaug gguagacauu      660

guauuagaac acggagguug ugugacugua auggcgcaag auaaaccaac aguagacaua      720

gaauuaguaa cuacaacugu aaguaacaug gcagagguaa gaucguauug cuaugaagca      780

aguauaagug acauggcuuc agauucaaga uguccaacac aaggugaggc auaucuagac      840

aaacaaucag acacacaaua cguauguaaa agaacuuuag uagaccgcgg cuggggaaac      900

ggaugcggac uauuugguaa aggaaguuua guaacaugcg caaaauucgc auguucuaag      960

aaaaugacag gaaaaucaau acaaccugaa aaucuagaau auagaauaau gcuaagugua     1020

cauggaaguc aacauucagg aaugauugug aacgacacug gacaugaaac cgaugaaaau     1080

agagcaaaag uagaaauaac accaaauucg ccaagagcag aagcaacucu uggggguuuu     1140

ggaucauugg gauuagauug cgagccuaga acaggauuag auuucagcga ucuuuauuac     1200

uugacaauga auaauaaaca uugguuagua cauaaagaau gguuccauga uauaccacua     1260

ccauggcaug cgggagcaga uacugguaca ccucauugga acaauaaaga agcacuggua     1320

gaguucaaag augcccaugc aaaacgacaa acaguaguag uauuaggguc acaagaagga     1380

gccguucaca cagcacucgc uggcgcauua gaagccgaaa uggauggcgc aaaaggaaga     1440

uugucuucag gacaucuaaa augccgauua aaaauggaca aauuaagacu aaaaggggua     1500

agcuauucau uauguacagc ggcauuuaca uuuacaaaaa uaccugcaga aacucuacau     1560

ggaacaguaa caguggaagu acaauaugca gguacugacg guccuuguaa aguaccagca     1620

caaauggcag uagacaugca aacuuuaaca ccaguaggga gauuaauuac ugcgaaucca     1680

guuauaacag aaaguacaga aaauucaaaa augauguuag aauuagaucc accauucgga     1740

gauagcuaua uaguaauagg uguaggagaa aagaaaauaa cucaucacug gcacagaagu     1800

ggauccacaa uaggaaaagc auucgaagca acaguaagag gcgcaaaaag aauggcagua     1860

uuaggcgaca cugcauggga uuuuggguca guaggaggag cauugaauag ucucgguaaa     1920

gguauccauc aaauauuugg agccgccuuu aaaaguuuau ucggaggaau gucuugguuu     1980

ucucaaaucu uaauagguac acuacuaaug uggcucggac uaaauacgaa aaauggcucg     2040

auaucacuaa uguguuuagc ucuaggaggu guacuaauau uuuuaucaac agcagugagc     2100

gccugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  9180
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9180
gggagaaagc uuaccaugaa gaaaagaagg ggagcagaua caaguguagg aauaguagga       60

cuauuacuga caacagcaau ggccgcggaa guaacaagaa gaggaucagc uuacuacaug      120

uacuuagacc gaaaugaugc aggagaagca auaucguuuc caaccacauu aggaaugaau      180

aaauguuaua uacaaauaau ggauuuagga cauaugugug acgcaacaau gagcuaugaa      240

ugcccaaugc uagaugaagg agucgaaccu gaugauguag auuguuggug caacacaacu      300

agcacuuggg uaguauacgg aacaugucac cauaagaaag gugaagcuag acgaaguagg      360

agagcuguaa cauuacccag ucauagcaca agaaaauuac agacaagaag ccaaacaugg      420

uuagaaucaa gagaauacac aaaacaccua auaagaguag aaaauuggau auuuagaaau      480

ccaggcuucg cucuggcagc agcagcgaua gccuggcuac uaggaaguuc gacaagucaa      540

aaaguaauau auuuaguuau gauuuuacug auugcuccag cauauaguau aagauguaua      600

ggagucagua accgagauuu uguagaaggc augucaggag gaacaugggu agauauugua      660

uuagaacaug gagguugugu cacaguaaug gcacaagaca aaccaacagu agauauagaa      720

uuaguaacaa cuacaguaag uaauauggca gagguaagau cauauuguua ugaagcaucc      780

auaagugaua uggcaucaga uucaagaugu ccuacucagg gagaagcaua uuuagauaaa      840

caaagugaua cacaauaugu auguaaaagg acauuaguag accguggaug gggaaaugga      900

uguggacuau uuggaaaagg aucauuaguu acaugcgcaa aauuugcaug uucuaagaaa      960

augacaggua aaagcauaca accagaaaau uuagaauauc gcauuaugcu aucaguucau     1020

ggaucccaac auucagguau gaucgugaac gauacagggc augaaacaga ugaaaauaga     1080

gcaaaaguag aaauaacacc aaacagccca agagcagaag caacuuuagg cggcuuugga     1140

aguuuaggau uagacuguga accaagaaca gguuuagauu uuucagaucu auacuauuua     1200

acaaugaaca auaaacauug guuaguccau aaagaauggu uucacgacau acccuuacca     1260

uggcacgcgg gugcagauac agguacucca cauuggaaca acaaagaagc auuaguagaa     1320

uuuaaggacg cucaugcaaa aaggcaaacu gugguaguau uaggaucaca agagggagcu     1380

guucacacag cauuagcagg agcucuagaa gcagagaugg auggagcaaa aggaagauua     1440

aguucgggac aucuaaaaug ucgacuaaaa auggacaagc uaagauuaaa aggaguaagu     1500

uauagucuau gcacagcagc uuuuacauuu accaaaauac cagcugaaac gcuacauggg     1560

acaguaacag uagaaguaca auaugcaggu acagaugggc cuuguaaagu accagcacaa     1620

auggcaguag auaugcaaac acuaacacca guaggacgau uaauaacagc aaauccagug     1680

auuacugaaa gcacagaaaa caguaaaaug augcuagaau uagacccacc cuuuggagac     1740

aguuauauag uaauaggcgu aggagaaaag aaaauaacuc aucacuggca uagaagugga     1800

agcacaauag gaaaagcauu ugaagcaaca guuagaggag caaaacgaau ggcugugcua     1860

ggagauacag cuugggauuu uggcucagua ggaggggcau uaaauucauu agguaaagga     1920

auacaucaaa uauucggugc agcauuuaaa agucuauuug guggaaugag uugguuuuca     1980

caaauauuaa uagguacucu acuaaugugg uugggauuaa acacgaaaaa cggaucaauc     2040

agucuaaugu gccuagcgcu ugggggcgug uuaauauuuu uaaguacagc aguaagugca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  9181
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9181
gggagaaagc uuaccaugaa aagacgugga gcugauacaa gugucggcau uguaggucug       60

uuauuaacaa cagcaauggc cgcagaagua acaagaagag gaucagcaua uuauauguau      120

uuagauagaa augaugccgg agaagcaaua ucuuuuccua caacauuagg gaugaauaaa      180

ugcuauauuc aaauaaugga uuuagggcac augugugaug caacgaugag uuacgaaugc      240

ccaauguuag augaaggagu agaaccggac gauguagauu guugguguaa cacuacauca      300

acauggguug uuuacggaac augucaucau aagaaaggag aagcaaggag aagucgcaga      360

gcagucacau uaccuaguca cucaacaaga aaauuacaaa caagaaguca aacaugguua      420

gaaaguagag aauauaccaa acaucuaaua agagucgaaa auuggauuuu uagaaacccg      480

ggauucgcac uggcagcagc ugccauugca uggcuccugg gaucaaguac gucacaaaaa      540

guaauauauu uaguaaugau cuuauuaauc gcaccggcau acucaauuag auguauagga      600

guaagcaaca gagauuuugu agaagggaug ucagguggaa cauggguaga uaucguauua      660

gaacauggcg ggugugucac aguaauggca caagauaaac cuacaguuga uauagaauua      720

gugacaacca caguuaguaa uauggcagaa guacgaucau auuguuauga ggcuuccaua      780

ucagacaugg caagugacuc acgaugccca acucaaggag aagccuauuu agacaaacaa      840

ucugauacac aauacguaug caaaaggacc uuagucgaca gagguugggg aaauggaugc      900

ggacuauuug ggaaagguag cuuaguaaca ugugcaaaau uugcaugcuc gaagaaaaug      960

acaggaaaaa gcauacaacc agaaaauuua gaauauagaa uaauguuaag cguucaugga     1020

ucacaacaca guggaaugau aguaaaugau acagggcaug aaacagacga aaauagagcu     1080

aaaguagaaa uaacuccaaa cagcccaaga gcggaagcaa cauuaggagg auuuggaagu     1140

uuaggacuag auugcgaacc cagaacagga cuggacuuua gcgaccuaua uuauuuaaca     1200

augaauaaua aacauuggcu gguucauaaa gaaugguuuc augauauucc uuuaccgugg     1260

caugccggug cagauacagg uacaccacau uggaauaaca aagaagcacu cguagaauuu     1320

aaagaugcac augcaaaaag acaaacagua guagucuuag gaucccaaga aggagcagua     1380

cacacugcuc uagcgggagc ccuagaagca gaaauggacg gcgcuaaagg aagauuaucu     1440

ucaggacauc uaaaauguag acuaaaaaug gauaagcuaa gauuaaaagg cguaucuuau     1500

agccuaugua cugcagcuuu uacauuuaca aaaauaccag cagaaacuuu acauggaaca     1560

guaacaguag aaguacaaua cgcaggaacu gacggaccau gcaaaguucc cgcacaaaug     1620

gcagucgaca ugcagacacu aacaccaguu ggaagauuaa uaacagcaaa uccaguaaua     1680

acagaaucaa cagaaaauuc uaaaaugaug cuugaauuag acccgcccuu cggagauuca     1740

uauauaguaa uugguguagg agaaaagaaa auaacccauc acuggcacag aaguggaucc     1800

acaauaggua aagcauuuga agcaacagua agaggugcca aaaggauggc aguccuagga     1860

gauacagccu gggauuuugg aaguguuggu ggggcacuua acaguuuagg aaaaggaaua     1920

caucaaauuu uuggagcagc auucaaaagu cuauucggag gaaugaguug guuuucacaa     1980

auacuaaucg gaacacuauu aaugugguua ggauuaaaua cuaaaaacgg aagcauauca     2040

uuaauguguc uugcauuagg cggagugcua aucuuuuuaa gcacagcagu aagcgcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  9182
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9182
gggagaaagc uuaccaugag aaggggugcg gauacaucug uaggaauagu aggauuauua       60

uuaacaacgg caauggcagc agaaguuaca agaagaggaa gugcuuacua cauguauuua      120

gauagaaacg augcugguga agccauaagu uucccaacua cauuaggaau gaacaaaugc      180

uacauccaaa ucauggaccu uggacauaug ugugaugcua caaugucaua cgaaugucca      240

augcuggaug aagguguaga acccgaugau guagacuguu gguguaacac aacaucuacu      300

uggguuguuu augguacgug ucaccauaag aaaggugaag caagacgauc aagacgagca      360

guaacauuac ccagucacuc aaccagaaaa cuacaaacaa gaucacaaac augguuagaa      420

ucaagagaau auacaaaaca ucuuauaaga guagaaaacu ggaucuuuag aaacccugga      480

uuugcauuag cagcagcagc uauagcaugg uuacuagguu cgaguacaag ucaaaaaguu      540

auuuaucucg uaaugauacu auuaauagca ccagcuuaua gcauaagaug uauaggugua      600

ucaaauagag auuuuguaga agggauguca ggagggacau ggguagauau cguauuagaa      660

cauggaggau guguaacagu cauggcgcaa gacaaaccaa caguugauau agaauuagua      720

acaacaacag uuuccaauau ggcagaaguu agaucauauu guuaugaagc uagcaucuca      780

gauauggcuu ccgacucaag auguccaaca caaggagaag cauauuuaga caaacaaucu      840

gauacacaau auguauguaa aagaacucua guagauagag guuggggaaa ugguugugga      900

uuauuuggca aaggaucuuu aguuacaugc gcaaaauuug cauguaguaa gaaaaugaca      960

ggaaaaagca uucaaccuga aaauuuagaa uauagaauaa ugcuaucagu acacggaagu     1020

caacauagcg guaugauagu aaacgauaca ggacaugaaa cagaugaaaa cagagcaaaa     1080

guggaaauaa caccaaacuc accuagagca gaagcaacau uaggcggauu ugguaguuua     1140

ggauuagacu gugaaccaag aacaggguua gauuuuagug auuuguauua uuuaacaaug     1200

aauaacaaac auuggcuagu acauaaagaa ugguuccacg auauaccauu accguggcau     1260

gcaggggcag auacuggaac uccccauugg aacaauaaag aagccuuggu agaauuuaaa     1320

gacgcacaug caaaacgaca aacagucgua guauuaggcu cacaagaagg cgcaguacau     1380

accgcuuuag caggcgcuuu agaagcagaa auggauggag ccaaaggaag gcuaucaucu     1440

ggucaucuua aauguagacu aaaaauggau aaacuaagac uaaaaggagu uucuuacagu     1500

cucuguacag cagcauuuac auucacgaaa auaccagcug aaacacuaca uggaacugua     1560

acaguagaag uacaauaugc aggaaccgau ggaccaugua aaguaccagc acaaauggca     1620

guugauaugc aaacauuaac cccaguaggg agacuaauaa ccgcaaaucc aguuauuaca     1680

gaaucaacag aaaacaguaa aaugaugcua gaauuagauc ccccuuuugg cgauucauac     1740

aucguaauag gugugggaga aaagaaaaua acgcaucacu ggcaucgauc aggaagcaca     1800

auaggaaaag cauuugaagc uacagucaga ggagcuaaaa gaauggccgu ucuaggagac     1860

acugcuuggg acuucggauc aguuggugga gcauuaaauu cucugggaaa aggaauacau     1920

caaauauuug gugcagccuu caaaucacua uuugguggca ugaguugguu cucacagaua     1980

cuaauaggua ccuuauuaau guggcuagga uuaaauacua aaaacggauc aauaucauug     2040

augugcuuag cccuaggagg aguuuuaaua uuucuaagca cagcugucag ugcaugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  9183
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9183
gggagaaagc uuaccaugcg aggagccgau acaagcguug gaauaguugg uuuauuauua       60

acaacagcua uggcagcaga aguaaccaga cguggaucgg cauauuauau guaccuagac      120

aggaaugaug caggagaagc aauaucuuuu cccacaacuu uaggaaugaa uaaaugcuau      180

auacaaauaa uggauuuagg acauaugugu gaugcaacua ugagcuauga augcccuaug      240

uuggaugaag ggguugaacc agaugaugua gauuguuggu gcaacacuac aucaaccugg      300

guuguauaug gaacauguca ucacaagaaa ggagaagcuc gcagaagcag acgagcagua      360

acacuaccuu cacauagcac aagaaaacua caaacacgau cucaaacaug guuagaaagc      420

agagaauaca caaaacaccu aauaagggua gaaaauugga uauuuagaaa cccaggguuu      480

gcauuagcag cagcggcuau agccuggcua uuaggaucua guacaucaca aaaaguaauc      540

uacuuaguga ugauauuauu aauugcacca gcauacucaa uaagauguau aggaguuagc      600

aauagagauu ucgucgaagg aaugucaggc ggaacauggg uagauauugu auuggaacau      660

ggaggaugcg uaacaguaau ggcacaagau aaaccuacug uagacauaga auuaguaaca      720

acaacuguaa gcaacauggc agaaguaagg ucauauuguu acgaagcaag uauaucagau      780

auggcaagug auucgagaug uccaacacaa ggcgaagcau auuuagacaa acaaucagau      840

acacaauaug uauguaaaag aacccuagua gauagaggau ggggaaaugg auguggucua      900

uuuggaaaag gaaguuuagu aacaugcgcu aaauucgcau gcagcaagaa aaugacagga      960

aaaucuauac aaccagaaaa uuuagaauac aggauaaugu uaucagucca cggaagucaa     1020

cauucaggaa ugauagucaa ugacacagga caugaaacug acgaaaauag agcaaaagua     1080

gaaauaacac caaacucacc aagagcagaa gcaacacuag guggcuucgg uucauuagga     1140

cuggacugug aaccaagaac aggauuagac uucagcgauu uauacuacuu aacuaugaau     1200

aacaaacauu ggcuaguaca uaaagaaugg uuccaugaca uaccauuacc uuggcaugcu     1260

ggugcagaua cuggaacacc acauuggaau aauaaagaag cgcuagugga guucaaagau     1320

gcacaugcua aacgacaaac aguaguagua uuaggcucac aagaaggagc cguccauaca     1380

gcacuugcag gagcacuuga agcagaaaug gauggagcaa aaggaagauu aucgaguggc     1440

cacuuaaaau gucgacuaaa aauggauaaa cuaagauuaa aaggaguuuc auauaguuua     1500

uguacagcag cauuuacauu caccaaaauu ccagcagaaa cauuacaugg aaccguaacu     1560

guggaaguac aauaugcagg aacagaugga cccuguaaag uaccagcaca aauggcaguc     1620

gauaugcaaa ccuuaacacc aguaggaaga uuaaucaccg caaacccugu gauaacagaa     1680

ucuacagaaa acaguaaaau gaugcuagaa uuggauccuc cauucggaga uuccuauaua     1740

guaauaggag uaggagaaaa gaaaauuacu caccacuggc acagaucagg uagcaccaua     1800

gguaaagcau uugaagcuac aguaagaggu gcaaaaagga uggcuguauu aggcgacaca     1860

gcaugggauu ucggaucagu uggaggcgcu uuaaacucac ucggaaaagg cauucaucaa     1920

auauuuggag cagcauuuaa aaguuuauuu ggaggaaugu caugguuuuc acaaauucua     1980

auaggaacau uacuaaugug gcugggucuc aauaccaaaa acggcaguau cagcuugaug     2040

ugucuagcau uaggaggcgu acuaauauuc cuaagcacag caguaucagc uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9184
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9184
gggagaaagc uuaccauggg cgcagauaca aguguaggaa uuguaggguu auuacuaaca       60

acagcaaugg cagcagaagu aacaagaaga gguagugcau auuauaugua ucuugauaga      120

aacgaugcag gagaagcuau uucauuucca acaacacuag gcaugaauaa auguuauaua      180

caaaucaugg auuuaggaca caugugcgau gcuacaauga guuaugaaug uccaaugcua      240

gacgaaggcg uugaaccaga ugauguagau ugcuggugua acacaacaag uacuugggua      300

guuuauggca ccugucauca caagaaagga gaagcgcgaa gaaguagacg agcaguaaca      360

uuaccaucac auagcacaag aaaauugcag acuagaaguc aaacauggcu ugaaucaaga      420

gaauauacaa aacauuuaau acgaguugaa aacuggauau uccgaaaucc agguuuugca      480

cuagcagcag cagcaauagc auggcuacug ggaucaagua cuagccaaaa aguaauauau      540

uuaguaauga uauuacuaau agcccccgcg uacaguauaa ggugcauagg uguaucgaau      600

agggauuuug uugaaggaau gagcggaggg acuugggugg auauaguacu agaacaugga      660

gguuguguua caguaauggc acaagauaaa ccaacaguug acauagaauu agugacaaca      720

acuguaagca auauggcuga aguaaggagc uauugcuaug aagcaaguau aucagauaug      780

gcaagugauu caagaugucc aacacaaggu gaagcauacu uagauaaaca auccgauacu      840

caauauguau guaaaagaac ucuaguagau cgaggauggg gaaacggaug uggauuauuu      900

ggaaaaggga guuuaguaac augcgcaaaa uuugcaugua guaagaaaau gacaggaaaa      960

ucaauacagc cagaaaaucu agaauaucgg auaaugcuau caguacaugg uagucaacau     1020

ucaggaauga uaguaaacga uacaggucac gaaacagacg aaaaucgagc aaaaguugaa     1080

auaacaccua acaguccacg agcugaagca acauuaggcg gauuuggauc ccuaggauua     1140

gacugugaac cgaggacagg acuagauuuu ucagaucuau auuauuuaac aaugaacaau     1200

aaacauuggc uaguucauaa agaaugguuu caugauauac cauuaccaug gcaugcuggu     1260

gcagacacag gaacaccuca uuggaauaau aaagaagcau uaguagaauu uaaagacgca     1320

cacgcaaaaa gacaaacagu uguaguauua ggaagccaag aaggagcugu acauacagca     1380

cuugcuggag cacuagaagc ugaaauggau ggugcuaaag gcagauuaag caguggucau     1440

uuaaaaugca gauugaaaau ggauaaauua cgauuaaaag gaguaucaua uuccuuaugc     1500

acagcagcau uuacauuuac aaaaauacca gcagaaacau uacauggaac aguaacagua     1560

gaaguccaau augcaggaac agacggacca ugcaaaguac cagcacaaau ggcaguugau     1620

augcagacac uaacaccagu aggaagauua auaacugcaa acccgguaau aaccgaaagu     1680

acagaaaauu caaaaaugau guuggagcug gauccaccau uuggggacuc auacauagua     1740

auuggcguag gagaaaagaa aauuacacac cacuggcauc gaaguggaag cacaauagga     1800

aaagcauucg aagccacagu aaggggagca aaaagaaugg caguauuagg ugacacagca     1860

ugggacuucg guucaguagg aggugcuuua aauaguuuag guaaaggaau acaccaaaua     1920

uuuggagcag cauuuaaauc auuauuugga ggaaugaguu gguuuucaca aauucugaua     1980

ggaacguuac uaauguggcu uggcuuaaau acgaaaaaug gaagcauuuc auuaaugugu     2040

uuagcgcuug guggaguuuu aauauuuuua ucuacugcag uuagcgcgug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  9185
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9185
gggagaaagc uuaccauggc agauacgucu gucggaauag uuggauuacu acuaacaacu       60

gcaauggccg cagaagugac uagacgaggu ucggcguacu acauguaucu agacagaaau      120

gaugcuggag aagccauauc auuuccaacu acauuaggaa ugaauaaaug cuacauacaa      180

auuauggacc uaggacauau gugugacgca acaaugucau augaaugucc aauguuagau      240

gaagggguag aaccagacga cguagauugc ugguguaaca caacaucuac augggugguu      300

uacgguacau gucaucauaa gaaaggugaa gcaagacgcu caagacgagc aguaacacug      360

ccaucacaua guacaagaaa acuacaaaca agaucacaaa cauggcucga aucgagagaa      420

uacacaaaac aucuuauaag aguagaaaau uggauauuca gaaaucccgg cuucgcacua      480

gcugcagcag caauagcaug guuacuugga aguucuacga gucaaaaagu aauauaucua      540

guaaugauac uucuaaucgc gccugcauau agcauaaggu guaucggggu cucaaauaga      600

gacuuuguag aaggaauguc aggaggaaca uggguagaua uuguguuaga acauggugga      660

ugugucacag uaauggcaca agacaaaccc acaguagaca uagaauuagu uacuaccaca      720

guaucaaaca uggcagaagu ucgaucguau uguuaugaag cauccauaag ugauauggca      780

ucagacagua gaugcccuac ccaaggagaa gcauauuuag auaaacaaag cgauacucaa      840

uacguaugua aaagaacccu aguagaucgu ggauggggua acggaugugg uuuauuuggu      900

aaaggcaguu uaguaacuug ugcaaaguuu gcauguucaa agaaaaugac agguaaaucu      960

auacaaccgg aaaaccuaga auaucgaaua augcuaagug uacauggauc ucagcauagu     1020

ggaaugauag uaaaugacac agggcaugaa acagaugaaa acagagcgaa aguagaaaua     1080

acuccaaaca gcccaagagc agaagcaaca uuaggaggau uuggcagucu aggacuagau     1140

ugugaaccaa gaacuggauu agauuuuuca gaucuuuacu acuuaacuau gaacaacaaa     1200

cauugguuag uacacaaaga augguuucau gacauaccgc uaccauggca cgcuggugca     1260

gauaccggca caccucauug gaauaauaaa gaagccuuag uagaauuuaa agacgcacau     1320

gcaaaaagac aaacaguagu aguacuagga ucacaggaag gagcaguaca uacagcccua     1380

gcuggagcau uagaagcgga aauggacgga gcuaaaggaa gauugucaag cggacacuua     1440

aaaugucgac uaaaaaugga uaaacuaaga cuuaaaggag uaucauauag cuuaugcaca     1500

gcagcauuua cauuuacaaa aauaccggca gaaaccuugc augggacugu aacaguagaa     1560

guacaauaug cagggacaga uggcccaugu aaaguaccag cacaaauggc aguagauaug     1620

caaacccuaa cuccaguagg aagauuaaua accgcaaauc caguaauaac ugaaagcacg     1680

gaaaacucaa aaaugaugcu agaacuagau ccaccauucg gagacaguua uauaguaaua     1740

ggcguaggag aaaagaaaau aacgcaccau uggcauagaa gcggaaguac aaucggaaaa     1800

gcauuugaag caacaguaag aggcgcaaaa agaauggcag uacuaggaga uacagcaugg     1860

gacuuuggau cgguaggagg ggcauuaaac aguuuaggga aaggaauaca ucaaauauuu     1920

ggagcagcau uuaaaucacu auucggagga augagcuggu ucucacaaau auuaauaggg     1980

acauuauuga uguggcuagg ccuuaacacu aaaaauggau caauaagucu aauguguuug     2040

gcauuaggcg gaguacuaau auuuuuauca acagcaguau cagcaugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  9186
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9186
gggagaaagc uuaccaugga uaccucaguu ggaauaguag gacugcuauu aacaacagcu       60

auggcugccg aaguaacaag aagaggaagc gcauacuaua uguacuuaga uagaaaugau      120

gcgggagaag caauaaguuu uccaaccaca cuaggaauga auaaauguua cauacaaaua      180

auggaucuag ggcauaugug ugaugcgaca augucauaug aauguccaau guuggaugaa      240

ggcguagaac ccgaugaugu agacuguugg uguaacacaa caagcacaug gguaguauau      300

ggaacauguc aucacaagaa aggagaagca cgaagaagua ggagagcagu uacauuacca      360

ucacauagua cuagaaaauu gcaaacuaga ucacaaacau gguuagaaag ccgcgaauau      420

acaaaacauu ugauaagagu agaaaauugg auuuuuagaa acccaggcuu cgcacuagcc      480

gcagcagcaa ucgcauggcu ucuaggaagc ucuaccucac aaaaaguaau auauuuggua      540

augauauuau uaauagcacc agcuuauagu auaagaugua ucggaguaag uaauagagau      600

uuuguagaag gaaugagugg aggaacaugg guagacauag ucuuagaaca uggaggaugu      660

guuacaguaa uggcgcaaga uaaaccaaca guagauauag aauuaguaac cacaacagua      720

aguaauaugg cagaaguaag aaguuauugu uaugaagcaa gcauuaguga uauggcauca      780

gauagucgau guccaacaca gggagaagca uacuuggaca aacaaaguga cacucaguau      840

guauguaaga gaacauuggu agauagagga ugggguaaug guuguggauu auucggaaaa      900

ggaucguugg ucaccugugc aaaauuugcu uguagcaaga aaaugaccgg aaaaucaauu      960

cagccagaaa acuuagaaua uagaauaaug uuaagcguuc auggaaguca acauagcggc     1020

augauaguaa augauacagg acacgaaaca gacgaaaaca gagcaaaagu agaaauuaca     1080

ccaaauaguc caagagcaga agcaacauua ggagguuuug gaucucuugg auuggauugu     1140

gaaccaagga ccggacugga uuuuucagac cuauauuacu uaacaaugaa uaauaaacau     1200

ugguuaguac acaaagaaug guuucacgac auuccauuac cuuggcacgc cggagcagau     1260

acugggacac cgcauuggaa uaauaaagaa gcauuaguag aauuuaaaga cgcacacgca     1320

aaacgacaaa caguaguugu ucuaggaagu caagaggggg caguucauac agccuuagca     1380

ggggcacuag aggcagaaau ggauggagcu aaaggaagac uaaguagugg acacuuaaaa     1440

uguagauuaa aaauggacaa auuacgucuc aagggagucu ccuauagcuu guguacagca     1500

gcauuuacuu ucacaaaaau accagcagaa acuuuacaug gaacaguaac cguagaagua     1560

caauaugcag gaacagaugg accaugcaaa guaccagcac aaauggcagu cgacaugcaa     1620

acacuaacac caguagggag acuaaucaca gcaaauccug uaauaacaga aucaacugaa     1680

aauagcaaga ugauguuaga auuagaucca ccauuuggag auucauacau aguaauugga     1740

guaggugaaa agaagauaac acaucacugg cacagaagug gaucaaccau aggaaaggcu     1800

uuugaagcaa ccguaagagg ugcaaaacga auggcuguuu uaggagacac agcaugggac     1860

uuugggucag uagguggagc auuaaauagu cuaggaaaag guauucauca aauauuugga     1920

gcagcuuuua aaaguuuauu cggaggaaug ucaugguuca gccaaauauu gauaggaacu     1980

uuauuaaugu ggcuaggauu gaauaccaaa aaugguagua uuagucuaau gugucuugcg     2040

uuaggaggag uauuaauauu uuuaagcaca gcaguaucag caugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  9187
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  9187
gggagaaagc uuaccaugac aagcguagga auaguuggau uauuacuaac aacagcaaug       60

gcagcugaag uaacuagaag aggaagcgca uauuauaugu accuagaucg aaacgacgcu      120

ggagaagcaa uaucuuuccc aacuacauua ggcaugaaua aaugcuauau acaaauaaug      180

gauuuaggac auaugugcga ugcaacaaug ucauacgaau gucccauguu agacgaagga      240

guagaaccag augauguaga uuguuggugc aauacaacau caacaugggu cgucuauggc      300

acaugucauc auaagaaagg ugaagcacgg agaagucgca gagcaguuac acuaccguca      360

cauucaacaa gaaaacuaca aacuagauca caaaccuggu uggaaaguag agaauauaca      420

aaacaucuaa uaaggguaga aaauuggaua uucagaaacc ccggauuugc uuuagcugca      480

gcagcaauag cauggcuacu aggaaguagc acaucacaaa aaguaauaua ucuaguaaug      540

auacuacuaa uagcgccagc guauaguaua agauguauag gaguaucuaa ccgugauuuu      600

guggaaggga ugucaggugg aacaugggua gauauuguau uagaacaugg aggaugugua      660

acagucaugg cgcaagacaa accaacugua gauauagagc uaguaaccac aacuguauca      720

aauauggcag aaguuagauc cuauugcuac gaagcaucga uuagcgauau ggcaagugau      780

uccagauguc caacccaagg agaggcuuac uuagauaaac aaagugauac acaauaugua      840

ugcaaaagaa cauuagucga uagaggaugg ggaaauggau gcggguuauu cggaaaagga      900

ucuuuaguga caugugcaaa auuugcuugu ucuaagaaaa ugacaggcaa aucaauacaa      960

ccagaaaacu uagaauauag aauuauguua ucaguacacg gaucacaaca uagcggaaug     1020

auugucaaug auacaggaca ugaaacagau gaaaauagag caaaaguaga aauaacaccu     1080

aauucaccua gagcagaagc aacacuagga ggauucggaa guuuagguuu ggauugcgaa     1140

ccacgaacug gucuagauuu uucagauuua uacuauuuga caaugaauaa uaaacauugg     1200

uugguacaua aagaaugguu ucaugacaua ccacuaccuu ggcaugcagg agcagacacu     1260

ggaacaccac acuggaacaa uaaagaagca cuugucgagu ucaaagaugc acaugcuaaa     1320

agacaaacag uuguuguauu aggaagccaa gaaggagcag uacauacagc auuagcaggg     1380

gcguuagaag cagaaaugga uggagcaaaa ggcagacuuu cuuccggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu acgccuaaaa ggugucucau acucauuaug uacagcagca     1500

uuuaccuuua ccaaaauucc agcggaaacc cuucauggaa ccguaacagu ugaaguucaa     1560

uaugcuggca cagacggacc augcaaagua ccugcacaaa uggcaguaga uaugcaaacu     1620

cuaacuccag uugggagacu aauaacagcc aauccaguua ucacagaaag cacagaaaau     1680

ucaaagauga ugcucgaauu agauccgcca uucggugacu cuuauauagu uauaggagua     1740

ggugaaaaga aaauaacaca ucauuggcac cguagcggaa gcacuauugg aaaagcauuu     1800

gaagcaacag uuagaggugc aaaaagaaug gcaguacuag gagauaccgc augggauuuc     1860

ggaagugucg gcggagcacu aaauucauua gguaaaggua uacaucaaau auuuggagca     1920

gcuuuuaaaa gccuauuugg uggaaugagu ugguucucgc aaauauuaau aggaacccuu     1980

uuaauguggc uaggacuaaa uacuaaaaau ggaaguauaa gccucaugug ucuagcacuu     2040

gggggaguau uaauauucuu auccacagcu guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9188
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9188
gggagaaagc uuaccaugac gucaguagga auugucggau uauuauuaac uacagcaaug       60

gcagcagcug uaaccagaag gggaucagcc uacuauaugu auuuggaccg aaaugaugcu      120

ggugaagcaa uaucauuucc aacuacauua gguaugaaua aaugcuauau acaaauaaug      180

gaucuuggac auauguguga ugcaaccaug aguuaugaau guccuauguu agaugaagga      240

guagaaccug augacguaga uugcuggugu aacacaacuu caacaugggu aguauaugga      300

acaugccauc acaagaaagg agaagcaaga cgaagccgga gagcaguaac auuaccaagu      360

cacucaacga gaaaauuaca aacaagauca caaacauggc uugaaaguag agaauacacu      420

aaacauuuaa uaagagucga aaacuggaua uuuagaaauc caggauuugc acuagcagcu      480

gcagccauug caugguuauu aggaucaagu acaucucaaa aagucauuua cuuaguaaug      540

auauuacuca uagcuccugc auauucaauc agaugcauag gcguuucaaa cagagauuuc      600

guagaaggaa ugucaggugg aacaugggua gauaucguac uagaacacgg aggaugcguu      660

acaguaaugg cacaggauaa accaaccgua gacauagaau uaguaacaac uacuguaagu      720

aauauggccg aaguaagauc auauuguuau gaagccagua uuagcgauau ggccagcgau      780

agcagauguc caacgcaagg agaagcauac uuagauaaac aaagugauac ucaauaugua      840

uguaaacgaa cauuaguaga ucgaggaugg gguaacggau gcgguuuauu uggaaaagga      900

ucauuaguaa caugcgcaaa auuugcaugc ucuaagaaaa ugacuggaaa aucaauacaa      960

ccagaaaauc uagaauacag aauaauguua ucgguacaug gaagccaaca uucaggaaug     1020

auaguaaacg acacuggaca ugaaaccgac gaaaauagag ccaaaguaga aauaacaccu     1080

aauuccccaa gagcagaagc aacauuagga ggauucggaa gccuaggacu agauugugaa     1140

ccaagaacug gucuugacuu cucagacuua uacuaccuaa ccaugaacaa caaacacugg     1200

uuaguacaua aagaaugguu ccacgacaua ccauuaccuu ggcacgcugg agcagacaca     1260

ggcacacccc acuggaauaa uaaagaggcu uuaguugagu ucaaagaugc ucacgcuaaa     1320

agacaaacag uaguaguacu agguucacaa gaaggugcag uacauacagc uuuagcagga     1380

gcacucgaag cagaaaugga uggagcaaaa ggaagauuau caaguggaca ucuaaagugc     1440

aggcuaaaaa uggauaaguu aaggcuaaaa ggaguaucau auucucuaug cacagcagca     1500

uuuacauuca caaaaauacc agcagaaacc uuacauggca ccguaacagu agaaguacaa     1560

uaugcaggua cagauggccc uuguaaaguc ccagcacaaa uggccguaga uaugcaaaca     1620

uuaacaccag ugggcagauu aauaacagca aaccccguaa uaacugaaag uacagaaaau     1680

aguaaaauga ugcuagaauu agauccucca uuuggcgaua guuauauagu aauaggagua     1740

ggagaaaaga aaauaacaca ucacuggcac cgcagcggaa guacuauugg aaaagcauuu     1800

gaagcaacug uaagaggagc aaaaagaaug gcuguucuag gagacacagc uugggacuuu     1860

gguucuguag gaggagcacu aaauaguuug ggaaaaggca uacaucaaau uuuuggcgcu     1920

gcauucaaau cacuauuugg aggaaugagu ugguuuucac aaauucucau aggaacauua     1980

uuaauguggc uaggguuaaa cacgaaaaau ggaaguauau cauuaaugug uuuagccuua     2040

gguggaguau uaaucuucuu aucuacagca guaucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9189
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9189
gggagaaagc uuaccaugcg uggagcugau accucaguug guauaguagg cuuacuauua       60

acaacagcaa uggcugccgc uguuacuaga agaggaucag cauauuauau guacuuagau      120

agaaaugaug caggagaagc aaucucauuu cccacuacuu uaggcaugaa uaaauguuac      180

auucaaauaa uggauuuagg acauaugugu gaugcaacaa ugucauacga augcccaaug      240

uuagaugaag gcguagagcc cgaugaugua gauuguuggu guaauacuac aucuacuugg      300

guaguguaug gaacauguca ucacaaaaaa ggagaagcaa gacgaagcag acgagcagua      360

acauugccau cucacucaac aagaaaauua caaacaagau cacaaacaug gcuagaaucu      420

agagaauaca caaaacauuu aauaagagua gaaaauugga uauuuagaaa uccaggauuc      480

gcguuggcgg ccgcagcgau agcaugguua uuaggaucaa guacaucaca aaaaguuauc      540

uauuuaguaa ugauccuacu aauagcacca gcguauagua uuagauguau aggcguauca      600

aauagagauu ucguagaggg gaugaguggu ggaacauggg uagauauagu uuuagaacau      660

ggugggugug uaacgguaau ggcucaagau aaaccaaccg uagauauaga guuagucaca      720

acuacaguca gcaauauggc cgaaguaaga ucauacuguu augaagcauc aaucucagau      780

auggccagug acaguagaug uccaacucaa ggagaagcuu aucucgacaa acaaagugau      840

acacaauaug uauguaaaag gacacuagug gauagagguu gggguaaugg uuguggacuc      900

uuuggcaaag guagucuagu cacgugcgcu aaauuugcuu gcucaaaaaa aaugacggga      960

aaaagcauac aaccugaaaa uuuagaauac cgaauaaugu uaucaguuca uggcagucaa     1020

cauagcggga ugauaguuaa cgauacagga caugaaacag augaaaauag agcaaaagug     1080

gaaauaacac cuaacucacc uagagcggaa gccacguuag gaggauuugg aaguuuagga     1140

uuagauugcg aaccaaggac aggauuagau uucucagauu uauauuaucu aacuaugaau     1200

aacaaacacu gguuaguaca uaaagagugg uuccacgaua uuccacuacc auggcaugcu     1260

ggggcagaca cuggaacacc ucauuggaau aauaaagaag cauuaguaga auucaaagac     1320

gcacaugcua aaagacaaac uguaguggua cuaggaaguc aagaaggagc aguacauacc     1380

gcacuugcug gagcacuaga agcagaaaug gauggugcaa aaggaagacu uucaagugga     1440

caucuaaaau guagauuaaa aauggacaaa uuaagauuaa aaggaguauc auacucauua     1500

uguacagcag ccuuuacauu cacaaaaaua ccugcagaaa cacuucaugg aacaguaaca     1560

guagaaguac aauaugcugg aacugauggc ccguguaaag uucccgcuca aauggcaguu     1620

gauaugcaaa cauuaacccc ugucgggaga uugauaacag cuaauccagu uauaacagaa     1680

ucuacugaaa acucaaaaau gauguuagaa uuagacccac cauucggaga uagcuauaua     1740

guaaucgggg uaggugaaaa aaaaauuaca caccauuggc acagaagugg aucuacaaua     1800

ggaaaagcau uugaagccac uguuagagga gcaaaacgaa uggcaguacu aggugacacc     1860

gcaugggauu uuggaucagu cggcggugca uuaaacucac uaggaaaagg aauccaucaa     1920

auauuuggag cugcauuuaa aagccuauuu gguggaaugu caugguuuag ccaaauacua     1980

auuggaacuu uauuaaugug gcuaggauua aauacaaaaa auggaucaau uagcuuaaug     2040

uguuuagccu uaggaggagu uuuaauauuc uuaagcacag cuguaagugc augaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9190
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9190
gggagaaagc uuaccaugag aggacuaucu agcagaaaaa gaagaucaca ugacguauua       60

accguucagu ucuuaauauu aggaauguua uuaaugacag gaggaguaac aagacguggc      120

uccgcguauu auauguaccu agauagaaau gaugcaggag aagcaauuuc auuuccuaca      180

acuuuaggaa ugaauaaaug cuauauacaa aucauggacc uaggacauau gugcgaugcc      240

acaaugagcu augaaugucc aauguuagau gaaggcguag aaccagauga cgucgauugc      300

ugguguaaca caacuucaac cuggguagua uauggaaccu gucaucauaa gaaaggagaa      360

gcaagacgau caaggcgagc aguaacauua ccaucucacu caacaagaaa auuacagaca      420

agaucacaaa cauggcuaga aaguagagaa uauacaaaac auuuaauaag agucgaaaau      480

uggaucuuuc guaauccugg uuuugcacua gcugcagcug cuauagcuug guuauuaggc      540

ucaagcacau cacaaaaagu uauuuacuua guaaugauau uacuaauagc accagcuuac      600

ucaauaagau guauaggugu uuccaacaga gauuuuguag aagguauguc uggaggaacu      660

uggguugaua ucgucuugga gcauggugga uguguaacgg uaauggcuca agauaaaccu      720

acaguagaua uagagcuagu uacgacaaca guuagcaaua uggcugaagu ccgaaguuau      780

ugcuacgaag caagcauaag ugauauggca agugauucaa gaugcccaac acaaggagaa      840

gcauaucuag auaaacaaag ugacacacaa uacguuugua aaagaacucu uguagauagg      900

gguuggggca auggaugugg uuuauucgga aaaggaaguu uaguaacaug ugcuaaauuu      960

gcuuguucua agaaaaugac uggcaaauca auucaaccag aaaauuuaga auacagaaua     1020

augcuaucag uucaugguuc acaacauucu ggaaugauag uaaaugauac aggacaugaa     1080

acagaugaaa aucgugcuaa aguagaaauu acaccaaacu caccaagagc agaagcaacu     1140

cuugguggau uuggcucauu gggguuggau ugcgaaccaa gaacuggauu agauuucagu     1200

gauuuguauu auuuaacuau gaauaauaaa cacuggcuag uucauaaaga gugguuccac     1260

gacauaccau uaccauggca cgcaggagca gauacaggaa caccacauug gaacaauaaa     1320

gaagcacucg uugaauuuaa agaugcacau gcaaaaagac aaaccguggu aguauuggga     1380

ucucaagaag gagcaguuca cacagcacua gcaggagcau uagaagccga aauggacgga     1440

gcaaaaggaa gguuaucuuc agggcaucua aaauguagau uaaaaaugga caaacuaaga     1500

uuaaaagggg uaaguuauuc acuauguaca gcagcuuuua cauucacaaa aauaccugca     1560

gaaacacugc acgggacagu aacaguagaa guacaauaug caggaacaga ugguccaugu     1620

aagguacccg cacaaauggc aguugacaug caaacucuaa cgccaguagg aagacuaauc     1680

acagcuaauc caguaauaac agaaucaacu gaaaacucaa aaaugaugcu agagcucgau     1740

ccaccuuucg gagauucaua cauugugaua gguguuggag aaaagaaaau uacacaucau     1800

uggcauagau caggaucaac uauaggaaaa gcauuugaag ccaccguaag aggagcaaaa     1860

agaauggcug ugcuaggaga uacugccugg gauuuuggaa guguaggagg agcauuaaau     1920

ucacuaggga aaggaauaca ucaaauauuu ggagcagcau uuaaaucacu auuugguggu     1980

augagcuggu uuucacaaau ucuaauagga acauuauuaa ugugguuagg ucuaaacacu     2040

aaaaacggaa gcauaucuuu aaugugccua gcacuaggug ggguacucau auuucuaagc     2100

acugcuguga gugcaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9191
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9191
gggagaaagc uuaccaugag aggguuauca agucgaaaaa ggagaucaca ugauguuuua       60

acaguacaau uuuuaauuuu gggcaugcua cuuaugacug gagcaguaac aaggagagga      120

agugccuauu auauguaucu ugauagaaau gaugcaggag aagcaauuuc auuuccaaca      180

acauuaggaa ugaauaaaug uuauauacaa auaauggauu uaggacacau gugugaugca      240

acaaugucau acgaaugucc aaugcuagau gaaggugugg aaccugauga uguagauugc      300

ugguguaaca caacaagcac uuggguagua uacggaacau gucaccacaa gaaaggggaa      360

gcaagaagaa guagaagggc aguuacccua ccaagccauu caacaagaaa auuacaaaca      420

agaucacaaa cauggcuaga aaguagagaa uacacaaaac acuuaauaag aguagaaaac      480

uggaucuuua gaaauccagg auucgcacua gcagcugcug cuauugcuug gcuauuagga      540

agcaguacua gccaaaaggu aauauaucuu guuaugauau uacuaauagc accagcauac      600

ucaauaaggu gcauaggagu aucaaauaga gauuuuguag aaggaauguc cggagguaca      660

uggguggaua uuguacuaga acauggcggc ugcgucacag ugauggcaca agacaaacca      720

acaguagaua uagaauuagu uacaacaaca gucucgaaua uggcagaagu aagaaguuau      780

uguuacgaag caagcauaag ugauauggca agcgacaguc ggugcccuac ucagggagaa      840

gcauauuuag auaaacaauc agauacccaa uacguaugua aacgaacucu aguagacaga      900

ggguggggaa auggaugugg acuauuuggu aaaggaucac ugguaacaug cgcaaaauuu      960

gcaugcagua agaaaaugac aggcaaaagu auucaaccug aaaauuugga auauagaaua     1020

augcuuagcg uacauggauc acaacacuca ggaaugauag ugaaugauac aggacacgaa     1080

acagaugaaa auagagcaaa aguagaaaua acaccuaaua guccuagagc ggaagcaaca     1140

cuaggaggau ucggaucauu gggucuugac ugugaaccac gaacuggguu agauuuuuca     1200

gaucuauauu auuuaacaau gaauaauaaa cauugguuag uacacaaaga gugguuccau     1260

gauauaccau ugccauggca ugcaggagcg gauacuggaa caccgcauug gaacaacaaa     1320

gaagcacuug uagaauuuaa agaugcacau gcaaaaaggc aaacaguagu aguacuugga     1380

ucucaagaag gagcaguaca uacugcuuua gcaggagcau uagaagcuga aauggaugga     1440

gcaaaaggua gacuaagcuc gggacacuua aaaugcagac uaaagaugga uaaauuaaga     1500

uuaaaaggug ugucguacag uuuauguaca gcugcauuua ccuuuacuaa aauaccagca     1560

gaaacauuac auggcacugu aacaguugaa guacaauaug caggaaccga ugguccaugc     1620

aaaguaccag cucaaauggc aguagauaug caaacgcuua cuccaguagg aagguuaaua     1680

acagcuaauc caguaauaac ugaauccacc gaaaauagca aaaugauguu agaauuagau     1740

ccaccauuug gggauucaua uauaguuaua ggaguaggag aaaagaaaau aacucaccau     1800

uggcacagau caggaaguac aauaggaaaa gcguucgaag caacaguaag gggagcaaaa     1860

cgaauggcag uauuaggaga cacagcaugg gacuuugguu caguaggcgg agcauugaau     1920

agcuuaggaa aaggaauaca ccagauauuu ggcgcagcuu ucaagagcuu auuuggagga     1980

augucguggu uuagccaaau acuaauagga acuuuauuga uguggcuagg acugaacacu     2040

aaaaacggaa guauuucauu aauguguuua gcauuaggag gaguauuaau cuuuuuauca     2100

acagcaguaa gugcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9192
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 3 opt

<400>  9192
gggagaaagc uuaccaugcg uauaauaaau gcaagaaaag aaaagaaaag aagaggcgca       60

gauacaagcg uaggcauagu uggguuacug uuaacaacag cuauggcugc agaaguaaca      120

agaagaggua gcgcuuauua cauguauuua gacaggaaug acgcuggaga agcaauuagc      180

uuuccaacaa cuuuaggaau gaauaaaugu uauauucaaa uaauggaucu aggacacaug      240

ugcgacgcaa ccaugaguua ugaaugucca augcuagacg aaggaguaga acccgaugau      300

guugauuguu gguguaauac aacgucaacc uggguuguau auggcacaug ucaucauaag      360

aaaggagaag caagaagaag uaggagagca guaacacuac cuucacauuc aacuagaaaa      420

cuacaaacuc guagccaaac auggcuagaa ucacgagaau auacaaaaca ucugauaaga      480

guagaaaauu ggauuuuuag aaauccugga uuugcucuag cugcagccgc aauagcaugg      540

cuacuaggau cuaguacuag ccaaaaagug auauacuuag uaaugauauu acuaauagcu      600

ccugccuaca guauacgcug cauaggagua agcaauaggg acuucguaga gggaauguca      660

ggaggcacau ggguagauau aguacuagaa cacggaggau guguaacagu aauggcccag      720

gauaaaccaa cuguagauau ugagcuagua acuacaacug uaucaaacau ggcugaaguc      780

agaaguuacu guuacgaagc aaguauaagu gauauggccu cagauucacg auguccaacu      840

caaggagaag ccuaucuaga uaaacaauca gauacucaau auguauguaa aagaacuuua      900

guagauagag gcuggggaaa uggcugugga uuauuuggaa aaggcagcuu agucacaugc      960

gcaaaauuug ccugcagcaa gaaaaugacg ggaaaaucga uacaaccgga aaauuuagaa     1020

uauagaauaa uguuaucagu acacgggagc caacauucag gaaugauagu aaaugauaca     1080

ggacacgaaa cggaugaaaa uagagccaaa guugaaauaa caccuaacag uccaagagca     1140

gaagcuacac uagguggauu cggcucauua ggauuggauu gugaaccacg aacuggcuua     1200

gacuucucug acuuauacua cuuaacuaug aauaauaaac auuggcuagu acauaaagaa     1260

ugguuccaug acaucccuuu accauggcau gcaggagcag auacaggaac accucauugg     1320

aauaauaaag aagcacuugu agaguucaaa gaugcacaug cuaaaagaca aacaguagua     1380

guauuaggau cacaagaagg ugccguacac acugcgcuag cuggugcauu agaagcggaa     1440

auggauggag ccaaaggaag acuaucuucc ggacaucuua aauguagauu aaaaauggau     1500

aaacuaagau uaaaaggagu aagcuauagu uuaugcacag cagcuuuuac auuuacaaaa     1560

auaccggcag aaacauuaca uggaacagua acaguugaag uucaauaugc gggaacggau     1620

ggaccuugca aagucccagc ccaaauggcu guggacaugc aaacauuaac gccaguagga     1680

agacucauua ccgcaaaucc aguaauaacu gaaucaacgg aaaacaguaa aaugaugcua     1740

gaacuagacc cuccauuugg agacagcuau auaguaauag gaguaggaga aaagaaaaua     1800

acacaucauu ggcaucgauc uggaaguacc aucggaaaag cauuugaagc gacaguacga     1860

ggagccaaaa gaauggccgu auuaggcgau acagcuuggg auuucgguuc ggucggaggc     1920

gcauuaaaua guuuaggaaa aggaauacau caaauuuucg gagcagcauu caaaagccua     1980

uuugggggca ugagcugguu uucacaaaua cucaucggua cuuuauuaau guggcuagga     2040

cucaauacga aaaaugguag uaucucauua auguguuuag cauuaggcgg aguauugauu     2100

uuucuuagua cagcaguaag ugccgacgua ggaugcucag uagauuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9193
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 3 opt

<400>  9193
gggagaaagc uuaccaugag aauuauaaac gcuagaaaag agaaaaagcg uagaggagcu       60

gauacaagcg uugggauagu agguuuguua cuaacaacag caauggcagc agaaguuaca      120

agaagaggga gugcauauua cauguaucua gacaggaacg augcugguga agcaauuuca      180

uucccaacaa cauuaggaau gaauaaaugu uauauacaaa uaauggacuu aggacacaca      240

ugcgaugcaa caaugucaua ugaguguccg auguuagaug agggaguaga accagaugac      300

guagauuguu gguguaauac aacaaguaca ugggucgugu auggaacaug ccaccauaag      360

aaaggagaag caagaagaag uagacgagca guaacauuac caagucacag uacaagaaaa      420

cuacaaacua gaagucaaac augguuagaa ucuagagagu auacaaaaca cuuaaucaga      480

guagaaaauu ggauauuuag aaauccagga uuugcauuag cagcugcggc aauagcuugg      540

cuauuaggaa gcucaacauc ucaaaaagua auauauuuag uaaugauacu auuaauagca      600

ccagcuuaua gcauaaggug uauuggagua ucaaauagag auuucguuga aggaauguca      660

ggaggaacau ggguagaugu uguucuagaa cauggagguu gcguuacagu cauggcacaa      720

gauaaaccaa caguagauau agaauuaguc acaacaacag uuaguaacau ggcagaagua      780

cgcucauauu guuaugaagc aagcauaagc gauauggcau cagauucaag auguccaaca      840

caaggagaag cauauuuaga uaaacaaucu gacacacaau auguauguaa acguacguua      900

guagaccgug gaugggggaa cggaugugga uuauuuggua aaggaucguu aguaacaugc      960

gcaaaauucg caugcaguaa gaaaaugacc ggaaaaagca uacaacccga gaacuuagaa     1020

uauagaauaa uguuaagcgu acauggcagc caacauagug gaaugaucgu caacgauaca     1080

ggacaugaaa cagaugaaaa uagagcuaaa guagaaauaa ccccaaacag cccaagagca     1140

gaagcaacau uagguggauu ugguucacua ggauuagauu gcgaaccuag aacgggccug     1200

gauuuuagug accuauacua uuuaacaaug aauaauaaac acugguuagu ucauaaagaa     1260

ugguuucaug auauuccucu accauggcau gcaggcgcag auacagguac accacacugg     1320

aacaauaaag aagcauuagu agaguucaaa gaugcacacg caaagagaca aacugucgug     1380

guacuaggua gucaagaagg ugcaguucau acagcacucg caggagcguu agaagcagaa     1440

auggauggag cgaaaggaag auugaguuca ggucaucuaa aaugccgacu aaaaauggau     1500

aaauuaaggu uaaaaggugu aucauauuca uuaugcacag ccgcuuucac auuuacaaaa     1560

aucccagcag aaacuuuaca uggaacagua acuguagaag ugcaauaugc cggaaccgau     1620

ggaccaugca aagucccugc ucaaauggcu guagacaugc aaacauuaac gccaguugga     1680

agacuaauaa cagcaaaccc cguaauaacg gaaagcaccg aaaacagcaa aaugaugcua     1740

gaauuagauc caccuuuugg agacaguuau aucguuauag gcguaggcga aaagaaaauu     1800

acccaucauu ggcauagaag uggaagcaca auuggcaaag cauucgaggc aacaguaaga     1860

ggcgccaaaa gaauggcugu cuuaggagac acagcauggg auuuuggaag ugucggagga     1920

gcauuaaaua gccuuggaaa aggaauacau caaauauuug gggcagcauu caaaucacua     1980

uuuggaggaa ugagcugguu uucacaaauu uuaauaggua cccuauuaau gugguuagga     2040

cuaaaugcua aaaauggaag uauaaguuua augugucuug cauuaggagg aguauuaaua     2100

uuucuaucaa cggcuguauc agcugaugua ggauguagug uagacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9194
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 3 opt

<400>  9194
gggagaaagc uuaccaugag aauaaucaac gcucgaaaag aacgaaaaag aagaggagca       60

gauacuagua uuggcauaau aggucuauua cuuacuaccg cuauggcagc ugaaauaaca      120

agaagaggaa gcgcauacua cauguauuua gauagaagug augcaggaaa agcaauuucu      180

uuugcaacga cauugggagu aaauaaaugc cacguucaaa uaauggaucu aggacauaug      240

ugugaugcua cuaugucaua cgaauguccg auguuagaug aaggaguaga accagacgac      300

gucgauuguu gguguaauac aaccagcaca uggguagugu acggaacaug ccaccauaaa      360

aaaggagagg caagacgaag caggcgagca guaacauugc cgagucacuc aacaagaaaa      420

cuacaaacua ggucacaaac augguuagaa aguagagaau auacaaaaca uuuaauaaaa      480

guggaaaauu ggauauuuag aaauccagga uucgcucuag uugcuguggc aauagcuugg      540

uuauuaggua guaguacuag ucaaaaaguc auauaucuag uuaugauucu acugauagca      600

ccagccuauu cuauaagaug uauaggagua ucuaacagag acuuuguaga agguaugucg      660

ggaggaacuu ggguagaugu aguacuagaa cauggagguu guguaacggu cauggcacaa      720

gauaaaccua cuguagacau agaauuagua acaaccaccg uaucgaacau ggcagaagua      780

agaucuuauu gcuaugaagc aaguauuagc gauauggcaa gcgacucaag augcccuacu      840

caaggagaag cauacuuaga caaacagucc gauacucaau auguguguaa aagaacuuua      900

guagauagag gauggggaaa cggcugugga cuauuuggca aaggaucauu agugacaugu      960

gcaaaauuua cauguagcaa aaagaugaca gguaaaagua uacaaccaga aaauuuagaa     1020

uacagaauaa uguuaagugu acauggcucu caacauagcg gaaugauagg auaugaaaca     1080

gaugaggaua gagcaaaagu agaaguaaca ccuaauaguc cucgugcaga agcaacucug     1140

gguggauucg gaagccuagg auuggauugu gaaccacgaa caggauuaga uuuuagugau     1200

cuauauuacu uaacaaugaa uaauaaacau uggcuagucc auaaagaaug guuucaugac     1260

auuccauuac cauggcacgc aggugcagau accggaacac cacacuggaa uaauaaagaa     1320

gcuuuaguag aauuuaaaga cgcgcaugca aagaggcaaa cggugguagu auugggauca     1380

caagagggag caguacauac agcucuagca ggagcacuug aagcagagau ggacggugca     1440

aaaggaagau uauuuucagg acacuuaaaa uguagacuca aaauggauaa auuaagguua     1500

aaaggaguca gcuauagcuu augcacagca gcauuuacau ucacaaaagu accagcagag     1560

acauuacaug ggacaguuac aguugaagua caauaugcgg gaacagaugg accauguaaa     1620

auacccgugc aaauggcagu ugacaugcaa acauuaacac cuguaggaag auuaauuaca     1680

gcgaacccag uaauaacaga aucuacagaa aauaguaaaa ugauguuaga acuagaccca     1740

ccauuuggag auaguuauau agugauagga gugggugaua aaaaaaucac acaucacugg     1800

cauaggagug gaucuacuau uggaaaagcu uuugaagcaa caguaagagg agcaaaaaga     1860

auggcaguuu uaggagauac agcaugggau uucggaagcg uaggaggggu auuuaauagc     1920

cuagggaaag guauucauca aauauuugga gccgcauuua aaagucuauu cgguggaaug     1980

aguugguucu cacagauauu aauaggaaca uuauuagucu gguuaggauu aaauacaaaa     2040

aaugggucaa uaucucugac augucuagca cuagguggag uaaugauauu ucuaaguaca     2100

gcaguaagcg cagaugucgg auguagcgua gacuuuucga aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  9195
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 3 opt

<400>  9195
gggagaaagc uuaccaugag aauaauaaau gcgagaaagg aaaaaaaacg acgaggagca       60

gauacaucag uuggaauagu aggcuuauua uuaacaacgg caauggccgc agaagugaca      120

agaagaggaa gugcauauua cauguauuua gauagaaacg augcuggaga agcuauaucu      180

uuuccaacca cauuaggaau gaauaaaugu uacauacaaa uaauggauuu aggucauaug      240

ugugaugcua caaugucaua ugagugucca auguuagaug aagguguaga accugaugac      300

guagauuguu ggugcaacac aacaaguaca uggguaguau auggaacaug ucaucauaaa      360

aaaggagaag ccagaagaag cagacgugcu guaacauuac ccucacauag cacaagaaaa      420

cuacaaaccc gaagucaaac gugguuagaa ucuagagaau acacaaaaca ucuuauaaga      480

guagagaauu ggauauuuag aaauccuggu uucgcauugg cagcagcagc aauugcgugg      540

cuacuaggau cgucuacaag ucaaaaagua auauacuuag uaaugauauu acuuauagcg      600

ccugcauaua guauaagaug uauaggagua agcaauagag auuuuguaga agguauguca      660

ggggguacgu ggguagaugu aguauuagaa caugguggau gcgucacagu aauggcacaa      720

gacaaaccua caguagauau ugaacuagua acaacaacag uaucaaauau ggccgaagua      780

agaucauauu guuaugaagc aucuauaagu gauauggcua gugacaguag auguccaaca      840

caaggugaag cauaucuaga uaaacaaagc gauacacaau auguguguaa aagaacauua      900

guagauagag gaugggguaa cggauguggu uuauuuggaa aaggauccuu aguaacgugu      960

gcaaaauuug cuuguagcaa aaaaaugaca ggaaaaucaa uccaaccaga aaauuuagaa     1020

uauagaauca uguuguccgu gcaugguagu caacacucug gaaugauugu aaaugauaca     1080

ggacaugaaa ccgaugaaaa uagagcaaaa guugaaauaa caccgaauag cccaagggca     1140

gaagccacau uaggaggauu uggcucguua ggucuagauu gugaaccaag aaccggauua     1200

gauuuuagug accuauauua ucuaacuaug aacaacaaac acugguuagu ucacaaagaa     1260

ugguuucacg auauacccuu acccuggcau gcuggggcag acacagguac accacauugg     1320

aauaauaaag aagcacuugu agaauucaaa gacgcucaug ccaagagaca aacuguaguu     1380

gugcucggua gucaggaagg ugcuguacac acagcacuug caggagcacu ugaagcagaa     1440

auggauggag caaaggguag auuaucuagu ggucauuuaa aauguagauu aaaaauggac     1500

aaauuaagau uaaaaggcgu aaguuauuca cuauguacug cagcauuuac auuuaccaaa     1560

auaccagcag aaacacuaca uggaacuguc acaguagaag uacaguaugc cggaacagau     1620

gguccaugua aagugccggc ucaaauggca guugauaugc aaacauuaac accaguagga     1680

agauuaauaa cagcaaaucc gguuauuaca gagucuacgg aaaauucgaa aaugaugcuu     1740

gaacuagacc caccauucgg ugacaguuac auugucauug guguagguga aaaaaaaaua     1800

acacaccauu ggcauagauc aggaaguaca auaggaaaag cauuugaagc uacagugaga     1860

ggagcgaaac gaauggcagu acucggugau acagcauggg auuuuggauc uguaggugga     1920

gcauuaaaua guuuaggaaa agguauacau caaauauuug gagcagcuuu uaaaucacua     1980

uuuggaggaa ugucaugguu uucucaaauu cucauaggua cacuauuaau guggcuaggc     2040

cuaaauacaa aaaacggaag cauaaguuua auguguuuag cuuuaggagg uguuuuaauu     2100

uuuuuaagua cagcgguauc agcagaugua ggauguucag uugauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9196
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 3 opt

<400>  9196
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag agcgaaaacg uagaggagca       60

gauacaagca uuggaaucau agguuuacua uugacaacag caauggcugc ugaaauaacu      120

agaagaggcu ccgccuauua uauguaccua gacaggucag acgcagguaa agccauaagc      180

uuugcaacaa cauuaggcgu aaauaaaugu cauguacaaa uaauggauuu aggacauaug      240

ugugacgcua caaugaguua ugaaugucca auguuagaug aaggagugga accagacgau      300

guugauuguu gguguaauac cacuaguaca uggguaguau augguacgug ucaccacaaa      360

aaaggcgaag caagaagauc cagaagagcg gucacauuac caucacauuc aacuagaaaa      420

cuacaaacaa gaucccaaac auggcuugaa agcagagagu acacuaaaca ccuaauaaaa      480

guagaaaauu ggauauucag aaacccugga uuugcauugg uagcaguagc aauagcaugg      540

uuauuaggaa guagcaccuc acaaaaagua auauaucuag uaaugauucu acuaauugca      600

ccagcauauu caauaagaug cauagguguu uccaauagag auuuuguaga aggaauguca      660

ggaggaacau ggguagacgu cguccuagaa cacggugggu gcguaacugu aauggcacag      720

gauaaaccaa cuguagauau agaauuagua acaacaacug uaucuaauau ggcagaagua      780

agaucauauu guuacgaagc aucaauaucu gauauggcaa gugauuccag augcccaaca      840

caaggagaag cauauuuaga uaaacaauca gauacacaau auguuuguaa aagaacccug      900

gucgaucgag guugggguaa uggauguggu cuuuuuggua aaggaagccu aguaacaugu      960

gcaaaauuua cauguagcaa aaaaaugaca ggaaaaucua uacaaccaga aaaucuugaa     1020

uauagaauaa uguuaagugu ucauggaagc caacauucag gcaugauagu gaaugauaca     1080

gguuaugaaa cagaugaaaa cagggcgaaa guagaaguaa caccaaacuc accacgagca     1140

gaagccacuu uaggaggauu cggaucauua ggacuagacu gugagccaag aacaggauug     1200

gauuuuagug aucuauacua uuuaacaaug aauaauaaac acugguuagu acacaaagaa     1260

ugguuccaug acauaccauu accuuggcac gccggagcag acacaggaac accccacugg     1320

aauaauaaag aagcgcuagu agaauuuaaa gaugcacacg ccaaaagaca aacugucgua     1380

guauuaggau cacaagaagg ugcaguacau acagcucucg caggagcauu agaagcagaa     1440

auggacggag ccaaagguaa acuuuuuuca ggacauuuaa aauguagauu aaaaauggau     1500

aaauugcgac uaaaaggugu aagcuauuca cuauguacag cagcauuuac auucacaaaa     1560

guacccgcag aaacacuaca uggaaccgug acaguagaag uucaauaugc aggaacagau     1620

ggcccaugua aaauaccugu acaaauggca guagauaugc aaacacuaac ucccgucggc     1680

agauuaauua cagcaaaucc aguaauaacu gaaaguacag aaaauucaaa aaugaugcua     1740

gaauuagauc cgccauucgg agauucauac auaguuauug gcgugggaga caaaaaaauu     1800

acacaucacu ggcauagaag cggaaguacc auaggaaaag cuuuugaagc aacaguucga     1860

ggagcaaaaa gaauggcagu auuaggugac acugccuggg auuucgguuc cguaggagga     1920

guuuucaauu cauuaggcaa agguauucac caaauauuug gugcggcauu caaaucauua     1980

uuuggaggua ugucaugguu uagucaaaua cuaauaggaa cauuacuagu augguuagga     2040

uuaaauacaa aaaacggguc cauaucuuua acauguuuag cucuaggugg cguuaugaua     2100

uuuuuaagua cugcaguaag ugcagauguu ggcugcucag ucgauuuuuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9197
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 3 opt

<400>  9197
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaagaaaaag aagaggggcu       60

gauacaagua uagguauagu aggacugcua uuaacaacgg caauggcugc agaaauaaca      120

aggaggggau cagcauauua cauguaucua gauagaucug augcgggaaa agcaauaucu      180

uuugcgacaa cuuuaggagu aaauaaaugc cauguacaga uaauggauuu aggacauaug      240

ugcgaugcga cuaugaguua ugaaugccca auguuagacg aagggguuga accagacgac      300

gucgauuguu gguguaauac aacgaguacu uggguaguau auggaacaug ccaucacaag      360

aaaggcgaag caagaagaag cagacgagcg guaacauuac caucacauuc aacaagaaaa      420

cuacaaacaa ggucacaaac auggcuagaa agcagagaau auacaaaaca uuuaaucaaa      480

guagaaaauu ggaucuucag aaauccaggu uuuacucuag uagccgucgc aauugcaugg      540

uuauuaggaa guaguacauc acaaaaagua auauauuuag uaaugauacu auuaauagcg      600

ccagcauaua guaucagaug uauaggagua agcaauagag auuuuguaga aggaauguca      660

ggagguacau ggguagacgu gguacuugaa cauggagguu guguuacugu aauggcacaa      720

gauaagccga cuguagauau agaauuaguu acaacaacug uaaguaauau ggcagaaguu      780

cgaaguuauu guuacgaagc aucgauaagu gauauggcau cagacucaag auguccuaca      840

caaggugaag cauaucuaga caaacaauca gauacacaau acguauguaa aagaacauua      900

guagaucgug gguggggaaa uggaugcgga uuguuuggaa aaggaagccu aguaacaugc      960

gcuaaauuua ccuguaguaa aaagaugacu ggaaaaucaa uucagccgga aaaccuagaa     1020

uacagaauaa ugcuaucagu acacggaagu cagcauucag guaugaucgu aaaugacgaa     1080

aauagagcua aaguagaagu gacgccuaac aguccaagag cagaagcuac gcuaggaggu     1140

uuugggaguc ucggauuaga uugugaaccu agaacaggau uggauuuuag cgauuuauac     1200

uacuuaacua ugaauaauaa acauuggcuu guacauaagg aaugguuuca ugacauacca     1260

uuaccauggc augcaggagc agauacaggu acaccccauu ggaauaauaa agaagcucua     1320

guugaguuca aggaugcaca ugcuaaaaga caaacagucg uaguauuggg gucacaagaa     1380

ggugcaguac auacagcucu ugcuggagca cuagaagcug aaauggaugg agcuaaagga     1440

agacuguuua guggucauuu aaaaugucga uuaaaaaugg acaaauuaag auugaaaggg     1500

guaagcuacu cuuuauguac ugcagcauuc acguucacua aaguaccggc ugaaacauua     1560

cauggaacag uaacaguaga aguacaauau gcggguacag auggaccaug uaaaguacca     1620

gcucaaaugg cgguagauau gcaaacacua acaccaguug ggagauugau aacagcgaac     1680

ccaguaauaa cggaaaguac agaaaauagu aaaaugaugu uggaacuuga cccaccauuc     1740

ggagauaguu auauaguuau cggaguagga gauaagaaaa uuacacauca uuggcauaga     1800

aguggaagua caauugggaa agcauuugaa gccacuguua gaggggcaaa aaggauggca     1860

guuuuaggag auacagcaug ggacuuugga agcguaggcg guguuuuuaa uagucuaggu     1920

aaagggauac aucaaauauu uggugcagca uucaaaaguu uauuuggagg gaugucaugg     1980

uucagucaaa uucuaauugg uacacuauua guaugguuag gucuaaauac gaaaaaugga     2040

aguauuucau ugacaugucu ugcauuaggu ggaguaauga uuuuccuaag uacagcuguu     2100

agugcagaug ugggauguag uguagauuuu ucaaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9198
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 3 opt

<400>  9198
gggagaaagc uuaccaugag aauaauaaac gcuagaaaag aaagaaaaag aagaggagca       60

gauacaucca uaggaauaau aggauuacuu cucacaacag cgauggcagc agaaaucaca      120

agacgcggaa gugcauacua uauguaucua gauagauccg augcaggaaa ggcgauauca      180

uuugcaacga cauuaggcgu uaauaaaugu cauguacaaa uuauggauuu gggccacaug      240

ugugaugcua ccaugaguua cgaauguccu augcuagaug aaggaguaga accugaugau      300

guggauuguu ggugcaacac aacaucaaca ugggugguuu augguacuug ccacagaaag      360

aaaggugaag caagaagaag cagacgagca guaacacuuc caucccauuc aacacggaaa      420

cuacaaacaa gaucacaaac augguuagaa ucuagagaau acacaaaaca ucuuauaaaa      480

guagaaaauu ggauuuuuag aaacccagga uuugcauuag uagcuguugc uauagcaugg      540

cuucuagguu caucaacauc acaaaaagua auuuauuuag uuaugauauu auuaauagcu      600

ccagcauaua guauuagaug uauuggagua ucgaauagag auuuuguuga agguaugagu      660

ggaggcacau ggguggacgu cguauuagaa cacgggggau guguaacagu aauggcacaa      720

gacaaaccaa caguugacau agagcuagua acuacaacag uaaguaacau ggcagaagua      780

agaagcuauu guuaugaagc caguaucagu gacauggcaa gugacaguag auguccuaca      840

caaggagagg cuuauuuaga uaaacaaagu gauacacagu auguauguaa aagaacauua      900

guagauagag guuggggaaa uggcugugga cucuuuggaa aaggaucgcu aguaacaugu      960

gcaaaauuua cauguucaaa gaaaaugacu ggaaaaucaa uucaaccaga aaacuuggaa     1020

uaccgaauaa ugcuaagcgu gcauggaucc caacauagcg ggaugauagu aaacgauaca     1080

ggauaugaaa cugacgaaaa cagagcaaaa guagaaguaa cuccaaauag uccaagagca     1140

gaagcaaccu uaggaggauu ugguucuuua ggacuagauu gcgaacccag aacaggauua     1200

gacuuuagug accuuuauua uuuaaccaug aauaacaaac auuggcuagu ucauaaagaa     1260

ugguuucaug auauaccauu accauggcau gcaggcgcag auacuggcac uccacauugg     1320

aacaauaaag aagcgcuagu agaauuuaaa gacgcucaug ccaagagaca aacagugguc     1380

guacuuggaa gccaagaagg cgcagugcau acagcucuag caggagcacu agaagcagaa     1440

auggauggug caaaaggaaa guuauuuagu ggacauuuaa aauguagacu aaaaauggau     1500

aaauuaagau uaaaaggagu cagcuacucg cucugcaccg cagcauuuac auuuacaaaa     1560

guaccagcag aaacauuaca uggaacaguu acaguugaag uacaauaugc agguacagau     1620

ggaccaugua aaauaccagu ucaaauggcu guagacaugc aaacauuaac gccuguaggg     1680

agacuaauaa cagcaaaucc agucauaacg gaaucaacag aaaacaguaa aaugaugcua     1740

gaacucgacc cuccauucgg agacucauac auaguaauug gaguuggaga caagaaaauu     1800

acucaccauu ggcacagauc agggucaaca auaggaaaag ccuucgaagc aacuguaaga     1860

ggagcuaaaa gaauggcugu auuaggcgac acagcauggg auuuuggauc cgucggagga     1920

guauuuaaua gcuuaggaaa aggaauacac caaauauuug gagcggcuuu uaaaucacuu     1980

uuuggcggaa ugucaugguu uucacaaaua cuuauaggaa cucuauuagu augguuaggc     2040

cuaaauacua aaaauggaag uauaucauua acaugucuag cauuaggagg agucaugaua     2100

uucuuaagca cagcaguaag ugccgacgua ggaugcucag uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9199
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 3 opt

<400>  9199
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaagaaaaag gagaggugcc       60

gauacgucua uagguauaau aggauuauua cuaacuacag caauggcggc agaaauaaca      120

cgaagaggaa gugcguacua cauguaucua gacagaagug acgcaggaaa agcaauuagc      180

uucgcaacaa cacuuggugu aaauaaaugu cauguccaaa uaauggauuu aggacauaug      240

ugugaugcca caaugucaua ugaaugucca augcuagaug aaggagugga gcccgaugac      300

guugacuguu gguguaauac aacaagcacu uggguaguau augguacuug ccaucauaag      360

aaaggugaag ccagaagaag cagacgagca guuacauuac caucacacag cacuagaaaa      420

uuacaaacaa ggagucagac uugguuagaa agucgagaau acacaaaaca ccuaauuaaa      480

guagaaaauu ggauauuuag aaacccuggc uucgcauuag uagcaguugc aauagcaugg      540

cuacuaggua guaguacaag ucagaaagua auuuauuuag ucaugauccu auugauagca      600

ccagcauauu caauaagaug uauugggguu ucaaauagag auuucguaga ggggaugagu      660

ggcggaacau ggguagacgu aguauuagaa cauggaggcu guguuacggu aauggcgcaa      720

gauaaaccaa cugucgauau agagcuagua acaacaacag uaucaaauau ggcagaggua      780

agaucuuacu guuaugaggc aaguauaagu gauauggcau cagacaguag auguccuaca      840

caaggagaag cauauuuaga uaaacaaucg gauacacaau auguauguaa aaggacgcua      900

guagaucgag gauggggaaa cggaugugga uuauucggaa aaggaaguuu aguuacaugc      960

gcaaaauuca cauguucaaa gaagaugaca ggaaaaucaa uacaaccaga aaaccuagaa     1020

uacagaauaa uguuaucugu acacggaagu cagcauucag gaaugacagu aaaugacaua     1080

ggauacgaga cugaugaaaa uagagcuaaa guagaaguaa cuccaaauuc accgagagca     1140

gaagcuacau uaggaggauu uggaagucua ggacuagacu gugaaccucg aacagguuua     1200

gauuuuagug auuuauauua cuuaacaaug aacaacaaac acuggcuagu ucauaaagaa     1260

ugguuucaug acauaccauu acccuggcac gcuggugccg auacaggaac cccacauugg     1320

aacaauaaag aagcauuagu agaauuuaaa gaugcacaug caaaaagaca aacaguagua     1380

gucuuggguu cacaagaagg cgccguacau acggcauuag caggggcuuu agaagcagag     1440

auggauggcg caaaagguaa auuauuuucg ggacaccuaa aaugcagauu aaaaauggau     1500

aaauuacgau uaaaaggagu aucauauuca cuauguacag cagcauuuac auuuacaaaa     1560

guaccagcug aaacucugca cggaacagug acgguagaag uucaauacgc cgguacagau     1620

ggaccaugca aaauaccugu acaaauggcu guagauaugc aaaccuuaac accaguagga     1680

agauuaauua cugcaaaucc aguaauuaca gagagcaccg agaauagcaa aaugaugcua     1740

gaauuagauc caccuuuugg ugauucauau auaguaaucg guguuggaga uaagaaaaua     1800

acacaccacu ggcauagauc aggaucaaca auagguaaag cauucgaagc aacaguaaga     1860

ggcgcaaaac gaauggcugu acuaggugau acagcuuggg auuuuggauc aguaggagga     1920

guauuuaaca gucuaggaaa aggaauacau cagauuuuug gggcagcauu caaaaguuua     1980

uuuggaggca ugagcugguu cagucaaaua uuaauuggaa cacuuuuagu auggcuagga     2040

uuaaauacga aaaaugguag uauaagucug acaugucuag cauuaggugg uguaaugaua     2100

uuucuuagca cagcggucag cgcagaugua ggauguucug uggacuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9200
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 3 opt

<400>  9200
gggagaaagc uuaccaugcg cauaauaaau gccagaaaag aaagaaaaag gagaggugca       60

gauacaucca uaggaauaau aggacuguug cuaacgaccg caauggcagc agaaauuaca      120

aggaggggcu cagcauauua uauguaucua gauaggucug augcaggaaa agcaauaagu      180

uuugcaacaa cacuaggggu aaauaaaugc cauguucaaa uaauggauuu aggacauaug      240

ugugaugcaa caaugaguua cgaaugcccu augcuagacg aaggagugga accggacgac      300

gucgauugcu gguguaauac aacuucaaca uggguuguau augguacuug ccaucauaaa      360

aaaggagaag caagaagaag ccgaagagca guaacacuac caagccauag cacucgaaaa      420

uugcaaacaa gaucucagac auggcuagaa ucaagagaau auacaaaaca uuuaaucaaa      480

guagaaaauu ggauauuuag aaauccagga uucgcccuag uagcaguggc aauagcaugg      540

uuauuaggau cuucaacaag ucaaaaaguc auauauuuag ugaugauuuu gcuaauagcu      600

ccagcauauu cuauaagaug uauaggagua ucaaacagag acuuuguuga aggaaugagu      660

ggcggaacau ggguagaugu aguauuagaa cauggaggau gugugacagu aauggcucaa      720

gauaaaccaa cuguugauau ugaacuugua acaacaacag uaucuaacau ggcugaagua      780

agaaguuauu guuaugaggc uucuauauca gauauggccu cagauaguag gugcccuaca      840

caaggagaag cauacuuaga uaaacaaagu gacacacaau acguauguaa acgaacuuua      900

guagauagag guuggggaaa uggcuguggu cuauuuggaa aaggaagucu aguaacaugu      960

gcaaaauuua cauguucaaa aaaaaugacu ggaaaaucaa uucaaccaga aaauuuagag     1020

uauagaauaa ugcuaucagu acauggaagu caacacagug ggaugauagg auacgaaaca     1080

gacgaaaaua gggccaaagu agaaguaaca ccgaacucuc caagagcgga agcgacauua     1140

gggggauucg gaucauuagg auuagauugu gaaccaagaa caggauuaga uuuuagcgau     1200

cuauauuacu uaacaaugaa uaacaaacau uggcuaguac auaaagaaug guuucaugau     1260

auaccucuac cauggcaugc aggagcagau acugguacgc cacauuggaa caauaaagaa     1320

gcauugguag aauuuaaaga ugcacaugcu aagaggcaaa cgguuguagu acucggauca     1380

caagaaggag caguacauac ugccuuagcu ggugcacuag aagcagaaau ggauggugca     1440

aaaggaaaac uauuuagugg acauuuaaaa ugucgacuaa aaauggacaa acuaagacua     1500

aaaggaguuu cauauucuuu augcacagcc gcauuuacau ucacaaaagu cccagcagaa     1560

acacuucacg gaacagugac aguugaagua caauaugcag gaacggaugg accguguaaa     1620

auaccuguac aaauggcagu agauaugcaa acacuaacac caguaggaag auugauaaca     1680

gcaaacccag uaauaacaga aucaacagaa aacucaaaaa ugauguuaga guuggauccg     1740

ccauucggag auaguuauau uguuauagga guaggcgaca agaaaauaac ucaucauugg     1800

cauagaucag gaagcacuau agguaaagcg uucgaggcaa cggugagagg ggcuaaaaga     1860

auggcaguau uaggagauac agcaugggau uuuggaagug uaggaggagu auucaacuca     1920

cuaggaaaag gaauacauca aauauuugga gcagcuuuua aaucacuauu cgguggcaug     1980

agcugguuuu cacaaauauu aauaggcaca cuauuaguau gguuaggacu aaacacaaaa     2040

aauggaagua uaagucuaac auguuuagca cuaggaggag uaaugauauu ucuaucaaca     2100

gcagugagug cugauguagg uugcaguguu gauuucucga aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  9201
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 3 opt

<400>  9201
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaagaaaag acguggagcu       60

gauacaucag uaggaauagu aggauuacua uuaacaacug cgauggccgc agagguaaca      120

cgaagaggau cagccuacua uauguaucug gacagaaaug augcaggaga agcaauaagu      180

uuuccaacua cauuagguau gaauaaaugu uauauacaaa uuauggacuu aggacauaug      240

ugcgaugcua caaugucaua cgaaugccca auguuagaug aagguguaga acccgacgau      300

guugauuguu gguguaacac aacaaguaca uggguaguuu acgggaccug ucaccauaag      360

aaaggagaag cgagaagauc aaggagagca gucacucugc caagccacuc uacaagaaaa      420

uugcaaacaa gaagucaaac auggcuagaa aguagggaau acacaaaaca uuuaaucaga      480

guagaaaacu ggauauuuag gaauccaggc uuugcacuag cagcagcagc aaucgcuugg      540

uugcuaggua gcaguacaag ucagaaagua auauauuuag uaaugauuuu auuaauagca      600

ccagccuauu caauucgaug uauagguguc agcaauagag auuuuguaga aggaaugucu      660

ggcggaacau ggguugaugu aguacuugaa cauggaggau gugugacagu aauggcucaa      720

gacaaaccaa cuguagacau agaauuagua accacuacug uaaguaauau ggcagaagua      780

agaaguuauu guuaugaggc uucaauauca gauauggcau cagacucaag auguccaaca      840

caaggagaag cauaucuuga caaacaauca gauacgcaau auguauguaa aagaacucua      900

gucgauagag gaugggguaa cggaugugga uuauucggaa aagguagcuu gguaacuugu      960

gcaaaauuug cauguaguaa gaaaaugaca gggaaaagua uacaacccga aaaucuagaa     1020

uaucgaauaa uguuaagugu gcauggaucg caacauagug gaaugauagu aaaugauaca     1080

ggacaugaaa cagaugaaaa uagagcuaaa guagaaauaa caccaaauuc accgagagca     1140

gaagcaaccu uaggaggguu uggaaguuug ggauuagauu gugaacccag aacaggcuua     1200

gacuuuucag aucuauauua ccuaacuaug aacaauaaac auugguuagu acauaaagaa     1260

ugguuucaug auauaccuuu accauggcau gcaggagcag auacugguac accgcauugg     1320

aauaauaaag aagcauuagu cgaauuuaag gaugcacacg caaaaagaca aacaguagua     1380

guacuaggau cucaggaagg cgcagugcau acagcacucg cuggugcauu agaagcagaa     1440

auggauggag caaaaggaag auuaagcucg ggacaucuaa aauguagauu aaaaauggac     1500

aaauugagau uaaaaggugu aagcuacagu uuaugcacag ccgccuucac auucacaaaa     1560

auaccugcag aaacauuaca uggaaccguu acgguagaag uacaauacgc aggaacagau     1620

ggaccaugua aagucccagc acaaauggca guagauaugc aaacauuaac accaguaggu     1680

agauugauaa cugcaaaucc aguaauuacg gaaucgacag aaaauaguaa aaugaugcua     1740

gaauuagauc caccauucgg cgauaguuau auaguaauag gaguuggaga gaaaaagaua     1800

acacaucacu ggcaucgaag ugguucaaca auaggcaaag cauucgaagc aacagucaga     1860

ggagcaaaaa gaauggcugu auuaggcgac acagcguggg acuucggaag uguaggugga     1920

gcacuaaauu cauuaggaag aggaauacau caaauauuug gagcagcauu uaaaucacua     1980

uuuggaggua ugagcugguu uagucaaaua uuaauuggaa cauuauuaau gugguuagga     2040

cuaaauacua aaaaugguuc uauaaguuua auguguuuag cacuaggagg aguacuaauu     2100

uuucucagua cagcuguauc agcagauguu gguuguucag uugauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9202
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 3 opt

<400>  9202
gggagaaagc uuaccaugag aauaauaaac gcacgaaaag agaaaaagag aagaggcgca       60

gaugccagcg uaggaauagu aggauuacua cuaacaacug caauggcagc cgaagucacu      120

cgaagaggaa gugcauauua uauguauuua gauaggaaug augcagggga agcaauauca      180

uuccccacaa cauuaggaau gaauaaaugc uacauacaaa uaauggauuu aggacauaug      240

ugugacgcaa cgaugucaua cgaauguccu auguuagacg agggaguuga accagaugac      300

guagauugcu ggugcaauac aacaucaaca uggguuguau auggcacuug ucaccauaag      360

aaaggagaag cgagaagauc uaggagagca guaacacuac caucacauag uacgagaaaa      420

cuacaaacaa gaucucaaac augguuagaa ucaagagaau auacaaaaca cuuaauaaga      480

guagaaaacu ggauauucag aaacccagga uucgcauuag cagcagcagc aauugcgugg      540

uuacuaggaa guaguacaag ccaaaaaguu auauaucuug ucaugauucu auuaauagca      600

ccagcauaua guauuaggug cauaggcguu agcaacagag auuucguaga agggauguca      660

ggaggaacau ggguagaugu uguauuagag caugggggau gugucaccgu aauggcacag      720

gauaaaccua caguagacau cgaauuaguu acaacaacag uaucaaauau ggcagaagua      780

agaucuuauu gcuaugaagc aucaauauca gacauggcua gugauagccg augcccuaca      840

caaggagaag cauacuuaga uaaacaauca gauacacaau auguauguaa aagaacccua      900

guagauagag gauggggaaa uggaugugga uuauucggaa aaggaucauu gguaacuugu      960

gcaaaauuug cauguucaaa gaaaaugacu ggaaaaagca uacagccuga aaaucuugaa     1020

uacagaauaa ugcuuagugu acacggaucu caacauucag ggaugauagu aaacgauaca     1080

gggcaugaaa cagaugaaaa uagagcgaaa guagaaauua caccuaauag ucccagagcu     1140

gaagcaacac uaggaggauu cggaagccua ggacuugauu gugaaccccg uacaggauua     1200

gauuuuucag acuuauauua uuugacaaug aacaauaaac auuggcuagu ccacaaagaa     1260

ugguuccacg acauaccgcu acccuggcac gcaggagcgg acacugguac cccacauugg     1320

aauaauaaag aagcauuagu agaguucaaa gaugcacaug cuaaacgaca aacaguagua     1380

guacuuggaa gucaagaagg agcaguacau acagcauuag cgggugcacu agaagcagaa     1440

auggauggag caaaaggaag auuaaguagu gggcaucuaa aaugcagacu caaaauggau     1500

aaacuaaggu uaaaaggagu aucauauuca uuauguacag cagcuuuuac auuuacuaaa     1560

auaccugcag aaacguuaca uggaacggua acugucgaag uacaauaugc uggaaccgac     1620

ggaccaugca aaguaccugc acaaauggca guagauaugc aaacauuaac uccuguaggc     1680

agauugauaa cugcaaaccc ugucauaacg gaaucaacag aaaauaguaa aaugaugcuu     1740

gaauuagacc caccauuugg agauaguuau auaguaaucg gaguagggga aaagaaaaua     1800

acacaucacu ggcacagguc ggggucaaca auaggaaaag cguuugaagc aacaguaaga     1860

ggagcaaaaa ggauggcagu auuaggugac acagcuuggg auuuuggaag cguagguggu     1920

gcauuaaaua guuuaggaaa aggcauucau caaaucuucg gagcggcauu uaaaagucua     1980

uucggaggaa ugucuugguu uucucaaaua cuuauuggaa cuuuauuaau guggcuagga     2040

uuaaauacua aaaacgguag uauaaguuua augugccucg cauugggagg aguauuaaua     2100

uuucucagua cagcgguaag cgcagacgua ggguguagcg uagauuucuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9203
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 3 opt

<400>  9203
gggagaaagc uuaccaugag aaucauaaau gcucggaagg aaaaaaaaag aagaggagcc       60

gauacaagcg uaggaauagu aggauuacuu cuaacgacag cgauggcggc agaggucaca      120

aggagaggau ccgcauauua cauguacuug gauagaaaug augcaggaga agcaauauca      180

uuuccaacaa cacuaggaau gaacaaaugu uauauacaaa uaauggaucu aggacauaug      240

ugcgaugcua caaugucaua ugagugccca auguuggaug aaggaguaga gccagaugau      300

guagacuguu gguguaacac aacaucaacu uggguuguau acgggacgug ucaucauaaa      360

aaaggggagg cgaggagguc gagaagagca guaacguuac caucacauag cacaagaaaa      420

uuacaaacac gaagucaaac cuggcuagaa aguagagaau auacaaaaca uuuaauuaga      480

guagaaaauu ggauauuuag aaacccagga uuagcguuag cugcagcagc aauagcaugg      540

cuuuuaggua gcucgacaag ucaaaaaguu auauaucucg uuaugauacu cuuaauagca      600

ccugcauaua guauaagaug uauuggagua aguaaucgag acuuuguaga agguauguca      660

ggaggaacau gggucgaugu gguacuggaa cauggaggau guguaacugu uauggcacaa      720

gauaaaccaa caguagacau agaguuagua acaacaaccg uaucaaacau ggcagaagug      780

cguaguuauu gcuaugaagc gucgauauca gacauggcaa gugauagccg auguccaacu      840

caaggagagg cauauuuaga uaaacaaagu gauacucaau auguaugcaa aagaacacua      900

gucgauagag guugggguaa cggaugcgga uuauucggga aaggcucauu aguaacuugu      960

gcuaaauuug cuuguucaaa aaaaaugacu ggaaaaagca uucaaccaga gaauuuggaa     1020

uaucguauaa uguuaagugu ucauggauca caacacucag guaugauagu caaugauaca     1080

ggacaugaaa cagacgaaaa uagggcaaaa guagaaauaa caccaaauag uccuagagca     1140

gaagcaacau uaggcggauu ugggaguuua ggcuuagacu gugagccaag aacuggauua     1200

gacuuuagcg acuuauauua cuuaacaaug aacaacaaac auugguuagu acacaaagag     1260

ugguuucaug auauaccguu accauggcau gcaggagcag acacuggaac accacauugg     1320

aauaauaaag aggcauuggu agaauuuaaa gaugcccaug cgaaacgaca aacaguggua     1380

guacuaggau cacaagaagg cgcaguacau acagcacuug cuggcgccuu ggaagcagaa     1440

auggacgggg cuaaagguag auuaaguuca ggucauuuaa aauguagauu aaaaauggac     1500

aaauuaagau uaaaaggugu gucauauuca uugugcacag cagcauucac auuuacuaaa     1560

auaccagcug aaacauuaca cgguacagua acaguagaag uucaauaugc agguacggac     1620

ggcccaugua aagucccagc ucaaauggcg guagauaugc agacauuaac uccaguuggu     1680

agacuaauaa cagcaaaucc uguaaucacc gaaaguacug aaaauucaaa aaugauguug     1740

gaacuugauc caccauuugg agauucauau auuguaauag guguagggga aaaaaaaaua     1800

acacaucauu ggcacagaag uggaaguacg auuggaaaag ccuuugaagc aacgguaagg     1860

ggugcaaaac gaauggcagu auuaggugau acagcauggg auuuuggcag uguaggaggu     1920

gcacuaaauu cauuaggaaa aggaauacau caaauauucg gagcagcauu uaaaagcuug     1980

uuugguggua ugucaugguu uucacaaaua cuaauaggaa cacuauuaau gugguuagga     2040

uuaaauacaa aaaaugggag uauaagucuc augugucugg cauuaggcgg aguacucaua     2100

uuuuuaagua cagcaguuag cgcagaugua ggauguagug uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9204
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 3 opt

<400>  9204
gggagaaagc uuaccaugcg aauaauaaau gcacgaaaag aaaagaaaag gagaggagcu       60

gacacuuccg uaggcauugu aggauuacuc uuaacaacug cuauggcagc ugaagucacc      120

agaagagggu cagcauauua cauguaucuu gauagaaaug acgcagggga agcaauuagc      180

uuuccaacaa cacuuggaau gaauaaaugu uacauacaaa uaauggaccu cggacauaug      240

ugcgacgcaa cuaugagcua ugaaugucca augcuagacg aaggaguaga accagaugac      300

guagacuguu ggugcaauac uacaaguacu uggguaguuu augggacaug ccaucauaag      360

aaaggugaag caagaagauc uaggagagca guuacauuac cuagucauag cacgagaaaa      420

cuacaaacaa gaucccaaac augguuagaa ucaagggaau acacaaaaca ucuuauaaga      480

guagaaaauu ggauauucag aaaucccggu uucgcucuag cagcagcugc uauagcuugg      540

cuauugggaa guagcacuag ccaaaaagua auauacuuag uaaugauacu auuaauagcu      600

ccugcauauu cuauaagaug uauuggagua aguaacagag acuucguuga aggaaugagu      660

ggaggcacau ggguagaugu cguacuugaa cacggaggau guguaacagu aauggcacaa      720

gauaaaccga caguagauau ugaauuggua acaacaacag ucaguaauau ggcagaaguu      780

agaaguuauu guuaugaagc uaguauaagu gacauggcau cagauucacg auguccuaca      840

caaggagagg cuuaucuaga caaacagucu gauacacaau acguguguaa aagaacuuua      900

guugaucgag gauggggaaa uggaugugga cuauuuggaa aaggaucauu aguaacaugu      960

gcaaaauucg cauguucaaa gaaaaugaca ggaaaaagca uacaaccaga aaauuuagaa     1020

uacagaauaa uguuaagugu gcauggaucc caacacagcg gcaugauagu aaaugauaca     1080

ggacacgaaa cagaugaaaa cagagcaaaa guugaaauua caccaaauag uccacgagcg     1140

gaagccacac uuggaggguu cggaucacua ggacuugacu gugaaccaag aacuggauua     1200

gauuucucag auuuauauua cuuaacaaug aauaacaaac auugguuggu acauaaagaa     1260

ugguuucacg auauuccgcu accauggcau gcuggagcag auacagggac accacauugg     1320

aauaacaaag aagcucuagu agaauuuaaa gaugcacaug caaagagaca aacaguagua     1380

guacuaggaa gucaggaagg agcaguacau acagcacuag caggagcucu agaagcugaa     1440

auggauggug caaaaggaag acuaaguagc ggacaucuaa aauguagacu aaaaauggac     1500

aaacuaagac uaaagggggu uucauauuca uuauguacag cagcuuuuac auucacaaaa     1560

auaccugcag aaacuuuaca ugguacagua acaguugaag uacaauaugc uggaacagac     1620

ggacccugua aaguaccagc acaaauggca guagauaugc aaacauuaac accaguugga     1680

cgauuaauaa cagcaaaucc cguuauaacc gaaaguacag aaaauucuaa aaugauguua     1740

gaauuagauc ccccauucgg agacagcuau auagugauug gaguaggaga aaagaaaauu     1800

acacaucacu gguaccgcuc aggaucaaca auaggaaaag ccuucgaagc uacuguaaga     1860

ggugcaaaac gaauggcagu acuaggggac acagcguggg auuucgguuc aguaggagga     1920

gcacuuaacu ccuuagguaa aggcauacau caaauauuug gagcagcuuu uaaaucucua     1980

uuugggggca ugucaugguu cucacaaauu uuaaucggca cacuacuaau gugguuagga     2040

cuaaauacua aaaacggauc aauaagucuc augugucuag cccuaggagg aguacuaaua     2100

uuucuaagua cugcuguauc agccgaugug ggaugcagug uagauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9205
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 3 opt

<400>  9205
gggagaaagc uuaccaugag aauaauuaau gcacgaaaag aaaaaaaaag aagaggagcu       60

gauacaagug uaggcauagu agguuuacug uuaacuaccg cuauggcagc agaaguaacg      120

agaagaggau cagcauauua uauguauuua gauagaaaug augcugggga agcaauauca      180

uuuccuacaa cauuaggaau gaauaaaugc uauauucaaa uaauggaucu gggacacaug      240

ugugaugcaa caaugucaua ugaaugcccc auguuagaug aagguguaga accagaugau      300

guagacuguu gguguaauac aacaucaacu uggguaguau auggaacaug ccaucauaaa      360

aaaggagaag cgagaagaag cagaagagcu guaacauuac caucacauag uacuagaaaa      420

cuacaaacaa gaagccaaac auggcuugaa aguagggaau auacuaaaca ucuuauaaga      480

guagaaaauu ggauauuuag aaauccagga uucgcauuag cagcugcagc uauagcaugg      540

cuauuaggau cauccacaag ucaaaaagua auauauuugg uaaugauauu acuaauugca      600

ccagcauacu caauaagaug uauaggaguc aguaauagag auuuugugga aggaaugagc      660

ggaggaacau ggguagacgu aguuuuagaa cacgguggau gcguuacagu uauggcacaa      720

gauaaaccaa cuguagacau agaauuaguu acaaccacag uaaguaauau ggcagagauu      780

agaucauauu guuaugaagc auccauaagc gauauggcau cggauaguag auguccaaca      840

cagggcgaag cauaccuaga uaaacaaagc gauacgcaau auguuuguaa aagaacgcua      900

guagauagag gaugggguaa ugguugugga cuuuucggaa aaggaagccu gguaaccugu      960

gcaaaauucg cauguucuaa aaaaaugaca ggaaaaucua uucaaccaga aaaucuggaa     1020

uauagaauaa uguuaagcgu ccauggaagu caacauuccg gaaugauugu aaaugauacg     1080

ggacaugaaa cagacgaaaa cagagcaaaa guugaaauaa caccaaauuc cccaagagca     1140

gaagcaaccc uaggaggauu uggaucauua ggauuagauu gcgaaccuag aacaggacua     1200

gacuucucag accuguauua ccuaacaaug aacaauaaac auugguuagu acauaaagaa     1260

ugguuccaug auauaccacu accguggcau gcaggagcug acacuggaac accccauugg     1320

aauaacaaag aagccuuagu agaguuuaaa gaugcacacg caaaacgaca aacaguaguc     1380

guccuaggua gucaagaagg ugcuguacau acagcauuag cuggugcucu agaagcagaa     1440

auggauggag cuaaaggaag acuaucaagu ggacaccuaa aaugccgauu aaaaauggau     1500

aaauuaagac uaaaaggagu aaguuacagu uuaugcacag cggcauuuac auuuacaaaa     1560

auacccgcag aaaccuuaca ugguacagua accguagaag uacaauaugc aggcacggau     1620

ggaccaugua aaguaccagc acaaauggcu guugauaugc aaacacuaac accgguaggc     1680

cgauuaauaa cugcuaaccc aguaaucaca gaaaguacug aaaauaguaa gaugauguua     1740

gaauuggacc caccauuugg agacucauau auaguaaucg gcguaggaga aaaaaaaauu     1800

acacaucacu ggcauagauc aggaaguaca auaggaaaag ccuucgaagc aacuguaaga     1860

ggagcaaaga gaauggcagu acuaggagau acagcauggg auuuuggauc gguuggagga     1920

gcucucaaua gcuugggaaa aggcauacau caaauauuug gagcagccuu uaaaaguuug     1980

uucggaggaa ugaguugguu uucucaaaua uuaaucggga cacuacuaau guggcuggga     2040

uuaaauacaa aaaauggcuc aauaagucuc augugcuuag cuuuaggggg cguuuuaaua     2100

uuuuuaagua cagcaguauc agcagacguu gguugcagcg uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9206
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 3 opt

<400>  9206
gggagaaagc uuaccaugag aauaauaaau gcccgaaaag aaaaaaaacg aagaggagcc       60

gacacaagug uaggaaucgu aggguuauua cuuacgacgg cuauggcggc agaaguaaca      120

cgaagaggaa gugccuacua cauguacuug gacagaaaug acgcaggaga agcaauauca      180

uuuccaacaa cacuagguau gaacaaaugu uauauucaaa ucauggaucu aggucauaug      240

ugugaugcca cuaugagcua cgaaugccca augcuugaug agggcguaga accugaugau      300

guggacuguu ggugcaacac aacuaguacu ugggucgugu acggcacaug ucaucauaaa      360

aaaggagaag caagaagaag caggagagca guaacguuac cgagccauuc aacaagaaaa      420

cuacaaacaa gaagccaaac augguuagag agucgagaau auacaaaaca cuugauaaga      480

guagaaaacu ggauauuuag aaauccaggu uucgcauuag cagcagcagc aauugcaugg      540

cuauugggua guuccacaag ucaaaaagua auuuauuuag uuaugauauu auuaauagcu      600

cccgcauacu caauaagaug cauaggagua agcaauagag auuuuguaga gggaaugucc      660

ggaggaacgu ggguagaugu agucuuagaa caugguggau guguaacugu aauggcacaa      720

gauaaaccaa cuguagauau cgaacuugua accacaaccg uaucaaauau ggcagaaguu      780

agaucguacu gcuaugaggc cucaauauca gauauggcau cagauucaag auguccaaca      840

caaggugaag cauaucuuga caaacaaucu gauacucaau auguauguaa aaggacacua      900

guagauagag guugggguaa uggcugugga cuauuuggua aaggaucucu aguaacaugu      960

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaagua uacaaccaga aaaucuagaa     1020

uacagaauaa uguuauccgu acaugggagc caacauagcg gaaugauagu aaaugauacu     1080

ggacacgaaa cagaugaaaa ucgagcaaaa guagaaauaa cucccaauuc accuagagcu     1140

gaagcaaccc uaggagguuu uggaaguuua ggauuagauu gugaaccacg aacaggucua     1200

gauuuuucag aucuauauua uuuaacaaug aauaacaaac acugguuagu acauaaagaa     1260

ugguuucaug auauaccauu accauggcac gcaggggcag acacaggaac uccccacugg     1320

aacaacaaag aagcuuuagu ggaauuuaaa gaugcacaug caaaaagaca aacaguagua     1380

guacuaggcu cucaagaagg agcugugcau acugcauuag caggagccuu agaagcagaa     1440

auggauggag caaaagggag auugucauca gggcauuuaa aaugucgacu aaaaauggau     1500

aaauuaagau uaaaaggggu cagcuauuca cuauguacgg cagcuuuuac auuuacaaaa     1560

auaccagcag aaacacucca ugguacagua acaguagaag uucaauaugc agguacagau     1620

ggaccaugua agguucuagc acaaauggca guagauaugc aaacguuaac uccuguagga     1680

agauuaauaa cagccaaucc uguaauaaca gaaucaaccg aaaauucaaa aaugauguua     1740

gaauuagauc cuccuuuugg agauucuuau auaguaauag guguugguga aaaaaaaaua     1800

acacaucauu ggcauagaag uggaagcaca auagguaaag cauuugaagc aacaguaaga     1860

ggagcaaaac gaauggcugu acuaggagac acagcuuggg auuucggaag uguaggcgga     1920

gcauuaaauu cauuaggaaa ggguauacau caaaucuuug gggcugcauu caaaaguuua     1980

uuugguggaa ugagcugguu cucacaaauu cuuaucggaa cacuauuaau guggcuaggu     2040

cuuaauacaa aaaauggguc aauaucuuua auguguuuag cauuaggggg aguacuuaua     2100

uuccucucaa cagccguauc agcagacguu ggauguucag uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9207
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 3 opt

<400>  9207
gggagaaagc uuaccaugag aauaauaaau gccagaaaag aaaaaaaaag acguggugcu       60

gacaccagug uaggaauagu aggauuauug uuaacaacug caauggcggc agaaguaaca      120

agaaggggaa gugcauauua uauguaucua gauagaaacg acgcaggcga agcaauaagu      180

uucccaacaa cauuaggaau gaacaaaugu uauauacaaa uaauggaccu cgggcacaug      240

ugcgaugcaa caaugaguua ugaaugccca acacuagacg aaggaguuga accggaugac      300

guagauuguu ggugcaacac aacaaguacg uggguuguau augguacgug ucaucauaaa      360

aaaggugaag ccaggagguc aagaagggca guaacauugc caucccauag uacacggaaa      420

uugcaaacaa gaucacagac uuggcuugaa agcagagaau auacaaaaca uuuaauaaga      480

guagaaaauu ggauauuucg gaauccagga uuugcauuag cagcugcugc aauugcaugg      540

cuucuagguu cgucaacauc acaaaaagua auauauuugg uaaugauauu guuaauugcu      600

ccagcauauu caauucguug uauaggugua agcaaucgug auuucguaga aggaaugagc      660

ggagggacgu ggguugaugu cguguuagaa cauggagggu guguaacagu aauggcacaa      720

gauaaaccaa caguagauau agaauuagua acaacuacag uaagcaacau ggcagaagua      780

agaucauauu guuaugaagc aaguauaucc gacauggccu ccgauaguag augucccaca      840

caaggugaag cguauuuaga uaagcaaucu gauacacaau acguaugcaa aagaacacua      900

guagauagag guuggggaaa uggcugcgga uuauuuggaa aaggcagucu aguuacaugc      960

gcaaaauuug cauguagcaa aaaaaugaca ggaaaaagua uucaaccaga aaacuuagag     1020

uauaggauaa uguuaucggu acacggaagu caacacucag gaaugauugu aaacgacacg     1080

ggccacgaaa cugacgaaaa uagagcaaaa guagaaauca cgccaaauuc accacgagcc     1140

gaagccacau uaggaggauu uggaucacug ggauuagauu gcgaaccuag aacgggauug     1200

gauuuuagug auuuauauua uuuaacuaug aauaauaaac auugguuagu acauaaagag     1260

ugguuucaug auauaccucu accuuggcau gccggggcag auacuggaac uccacauugg     1320

aauaauaaag aagcauuagu agaauuuaaa gaugcccacg caaaaagaca aacaguaguu     1380

guauuaggaa gucaggaagg agcuguacau acagccuuag caggagcacu agaagcagaa     1440

auggauggag caaaaggaag auuaucaagu ggacacuuaa aauguagauu aaaaauggac     1500

aaacuaagac uuaaaggugu gucauacucc uuauguacag ccgcuuuuac uuuuacuaaa     1560

auuccagcgg agacuuuaca ugggacugua acaguagaag uucaguaugc agguacagau     1620

gguccaugca aaguaccagc ccaaauggca guugauaugc aaacauuaac accaguagga     1680

agauuaauca cagccaaucc cguaaucaca gaauccacag aaaauaguaa gaugaugcua     1740

gaauuagacc caccguuugg agauaguuau auaguaauag gaguugggga aaagaaaaua     1800

acacaucauu ggcaccgaag cgguagcacc auaggaaaag cuuuugaagc aacaguuaga     1860

ggagcuaaaa gaauggcagu auugggugau acagcauggg auuucggauc aguaggugga     1920

gcauuaaaca gcuuggguaa aggaauacau caaauuuucg gagcagcauu uaaaagcuua     1980

uuuggaggaa ugaguugguu cucacaaaua uuaauaggaa cacuauuaau gugguuagga     2040

cuaaauacaa aaaauggaag uauaaguuua auguguuuag cauuaggggg aguacuaaua     2100

uucuuaucaa cagcaguauc ugcugacgua ggaugcucag uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9208
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 3 opt

<400>  9208
gggagaaagc uuaccaugag gauaauaaau gccagaaaag aaaagaaaag gagaggagca       60

gacacaagcg uaggaauagu aggcuuauua uuaacaacag caauggccgc agagguaacu      120

cgaagaggau cagcguacua uauguauuua gauagaaaug augcaggaga agcaauauca      180

uuuccaacaa cauuaggaau gaacaaaugc uacauacaaa uaauggauuu agggcauaug      240

ugugaugcca cuaugaguua ugaaugccca augcuagacg agggugugga accagaugac      300

guagauuguu gguguaacac aacaucuaca uggguuguau auggaacuug ucaucacaag      360

aaaggagaag cacgaagaag caggagggca guaacacuac caagccauuc aacuagaaaa      420

cuccaaacaa gaagucaaac cugguuagaa ucaagagaau auacgaaaca ucuaauaaga      480

guagaaaacu ggauauuuag aaaucccgga uuugcauuag cagcagcagc aaucgccugg      540

cuauuaggaa guucaacaag ucaaaaagug auauaucuag ucaugauacu acuaauugca      600

ccagcuuaua gcauaagaug uaucgguguc agcaauagag acuucguuga agguauguca      660

gguggaacgu ggguagacgu uguuuuagaa cacggcggau guguaacagu aauggcacaa      720

gauaaaccaa cgguagacau agaacuugug acaacuacug uuaguaacau ggcagaagua      780

cgaagcuauu gcuaugaagc aucuauauca gauauggcau cagauucacg auguccuaca      840

caaggagaag cauacuuaga uaaacaaagc gauacacaau auguuuguaa aaggacauua      900

guagauagag gcuggggaaa ugguugugga uuauuuggaa aagguucauu gguaacgugu      960

gcaaaauucg cuuguagcaa gaaaaugaca gguaaaucaa uacaaccuga aaacuuggaa     1020

uacagaauua ugcuaucagu acauggauca caacauuccg gaaugauagu aaaugauacu     1080

ggucaugaaa cagaugaaaa ccgagccaaa guagaaauaa caccuaauuc uccaagagca     1140

gaagcuacau uaggaggauu cggaucacuu ggucuagauu gcgaaccaag aacuggacuu     1200

gacuuuagug auuuauacua uuuaacaaug aauaauaaac auuggcuagu acauaaagaa     1260

ugguuucaug acauaccauu accauggcac gcaggagcag auaccggaac accacacugg     1320

aacaauaaag aagcucuagu agaguucaaa gaugcucaug caaaaagaca gaccguggua     1380

guauuaggau cacaagaagg agcuguacau acggcucuag caggagcacu ugaagcggaa     1440

auggauggug caaaaggacg auuaaguuca gggcaucuaa aauguagauu aaaaauggac     1500

aaauuaagau uaaaaggagu aaguuauagu uuaugcacag ccgcuuuuac auuuacuaaa     1560

auaccagcag aaacguuaca uggaaccguu accguagaag uacaauaugc aggaacagau     1620

gguccaugca agguaccagc acaaauggca guagauaugc aaacgcuaac accaguagga     1680

agguuaauca ccgcuaaucc agugauaaca gaaucgaccg aaaauuccaa aaugauguua     1740

gaauuggauc caccuuuugg agacuccuau auaguaauag ggguugggga aaagaaaaua     1800

acccaccauu ggcauagauc agggucaaca auaggaaagg cuuucgaggc aaccgugcga     1860

ggagcaaaaa gaauggcggu uuuaggggau acugcauggg auuuuggaag ugugggagga     1920

gcauuaaaca gccuggguaa agguauacau caaauauucg gagcagcauu caaaucauua     1980

uuuggaggaa ugagcugguu uagucagaua cuaauuggaa cauuacugau guggcuugga     2040

uuaaacacca aaaacggauc aauuagcuua augugcuuag cacuuggugg aguauuaaua     2100

uuccuaagua caguaguuuc agcagaugua gguugcucag uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9209
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 3 opt

<400>  9209
gggagaaagc uuaccaugag aauuauaaau gccagaaaag aaaaaaaaag aagaggcgcc       60

gauacaucag uagggauagu aggauugcua uuaacaacag ccauggcagc agaaguuaca      120

agacgaggau cagcauauua cauguacuua gauagaaaug augcaggaga agcuauaagu      180

uucccgacua cuuuaggaau gaacaagugu uauauucaaa uuauggacuu aggacacaug      240

ugugacgcaa caaugucaua ugaaugccca auguuagacg aaggaguaga accagaugau      300

guagauuguu gguguaacac uacaagcgcu ugggucgucu acgggacaug ccaccacaaa      360

aaaggagaag cuagacgaag uagaagagca guaacccuac caucucauag uacacgaaaa      420

cuacaaacua gaccucaaac cugguuagaa ucaagagaau acacaaaaca uuuaauacga      480

guagaaaauu ggauauucag aaacccagga uucgcguugg cagcggcggc aauugcaugg      540

uuauuaggau caaguacauc ucaaaaaguc auauauuugg uuaugauacu uuuaauagcu      600

ccagcauaua gcauacgaug uauaggagua agcaauagag acuuugugga aggaauguca      660

ggagggacau ggguugacgu agucuuagaa cauggaggau guguaacggu cauggcacaa      720

gauaaaccaa caguugauau agaauuagua acaacaacag uaucaaauau ggcagaaguu      780

agaucauauu guuaugaagc aucaaucuca gacauggcaa gugacucaag augucccacg      840

caaggugaag cauaucuaga caaacaaagu gauacacaau auguaugcaa aagaacacuu      900

guagacagag gauggggcaa cggaugcggu cucuuuggua agggcagcuu aguuacaugu      960

gcaaaauucg cguguaguaa aaaaaugaca gguaaaucaa uacagccuga aaaucuugaa     1020

uaucgaauua uguuaucugu ucacggauca caacauucug gcaugauugu aaaugacaca     1080

ggucacgaaa cagaugaaaa cagagcuaaa guagaaauaa caccgaacuc accaagagca     1140

gaagcuacuu uaggagguuu ugggaguuua ggacuagauu gcgaaccaag aacaggacuu     1200

gauuuuagug auuuauacua uuuaaccaug aauaauaaac auuggcuggu acauaaagaa     1260

ugguuucaug auauuccauu accauggcau gcaggagcug auacaggaac accccacugg     1320

aauaauaaag aagcgcuugu agaauuuaaa gacgcacaug ccaaaagaca aacaguagua     1380

guuuuaggua gucaagaagg ggcaguccau acagcacuag cgggagcauu agaagcagaa     1440

auggauggag caaaaggacg auuaagcuca ggacaucuaa aauguagacu gaaaauggau     1500

aaauuaaggc uaaaaggagu aagcuauagu cuuugcacag cagcauucac uuuuacaaaa     1560

auuccagcgg aaacacuaca uggaacugua acaguagaag ugcaauacgc aggaacugau     1620

ggaccgugua aaguuccagc acaaauggca guagauaugc aaacauuaac accaguagga     1680

cguuuaauaa cagccaaucc aguaauaaca gaaucaaccg aaaacucgaa aaugaugcua     1740

gaauuagauc cuccuuuugg agauucguac auaguuauag gugucggaga aaaaaagauc     1800

acacaucacu ggcauagaag cggcaguaca auaggaaaag cauuugaagc uaccguacgu     1860

ggagcuaaaa gaauggcagu ucuaggcgau acugcauggg auuuuggcuc aguuggaggu     1920

gcauuaaaca gcuuagggaa aggaauacau caaauuuuug gugcagcauu caaaagcuug     1980

uuuggaggaa ugucaugguu uagccaaaua uuaauuggaa cuuuacuuau gugguuagga     2040

cuaaauacaa aaaaugguag uauaucauua augugucuug cauuaggugg aguauugauu     2100

uuuuuaucaa cagcaguaag cgcagaugua ggauguucag uagacuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9210
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 3 opt

<400>  9210
gggagaaagc uuaccaugag aauaauaaau gcacgaaaag agaaaaagag acgaggcgca       60

gacacaagug uaggaauagu aggccuacuu uuaacuacag caauggcugc agaaguaacg      120

agacgagguu ccgcauauua uauguaccuu gacaggaaug acgcagggga agcaauaucc      180

uuuccaacca cauuaggaau gaauaaaugu uacauacaaa uaauggaucu aggacauaug      240

ugugaugcaa caaugaguua ugaaugucca augcuggaug aaggaguaga acccgaugac      300

guagauuguu ggugcaauac uacaucaaca uggguaguau augggaccug ucaucauaaa      360

aaaggagaag caagaaggag uagaagagca gucacauugc cgagucacuc aacaagaaaa      420

uuacaaacaa gaucccaaac auggcuugaa ucuagagaau acacaaaaca ucuuauaaga      480

guugaaaauu ggauuuucag aaaccccgga uucgcgcuag cagcagcagc aauagcaugg      540

cuccuuggau cauccacaag uaaaaaagua auauacuuag ugaugauauu acuaaucgca      600

ccagcauaca guauacguug cauaggagua ucaaaucgag auuucguuga agggaugucu      660

ggagguacau ggguagaugu uguacuagaa caugguggau guguuacagu aauggcgcaa      720

gacaaaccua cagucgauau agaacuagua acaacuacag uaagcaauau ggcagaaguc      780

cgaucauacu guuacgaagc aaguauaucu gacauggcaa gcgacagucg auguccaaca      840

caaggagaag cauaucuaga uaaacaaucu gacacacaau auguuugcaa aagaaccuua      900

guugauagag gaugggguaa uggguguggc uuauuuggca aggguagucu uguaaccugu      960

gcaaaauucg cauguaguaa aaaaaugaca ggaaaaagca uacaaccaga aaaucuagaa     1020

uauagaauaa uguuaucagu acaugguagu caacauucag gaaugauagu uaaugacacc     1080

ggacacgaaa cugaugaaaa ucgagcaaaa guagaaauaa caccaaauag uccacgagca     1140

gaagcuacau uaggcggauu uggaucguua ggauuagauu gugaaccaag aacuggauua     1200

gauuuuagug aucuauauua ccuaacaaug aauaauaaac acugguuagu acauaaagaa     1260

ugguuucacg acauaccacu acccuggcac gcuggagccg auacuggaac accacauugg     1320

aacaacaaag aagcacuagu agaguuuaaa gaugcacacg caaaaagaca aacaguagua     1380

guauuaggau cacaagaagg cgcaguccau acagcacuag caggagcauu agaagcggag     1440

auggacggag caaaaggaag acuaaguuca ggccauuuaa aaugcaggcu aaaaauggau     1500

aaauuaagau uaaaaggagu aucauauuca cuauguaccg ccgcauuuac auuuacaaaa     1560

auaccagcag aaacuuuaca uggcaccguu acugucgaag uccaauaugc uggaacagau     1620

ggaccaugua agguuccagc ucaaauggca guggauaugc aaacacuaac uccaguaggu     1680

agacuaauaa cagcaaaucc gguaauuacu gaaucaacag aaaacucaaa aaugaugcua     1740

gaauuagauc cuccguucgg agacucguac auaguuauag guguagguga aaaaaaaaua     1800

acacaucauu ggcauagauc aggaucaacc auagggaaag cuuuugaggc uacgguaaga     1860

ggagcaaaac gaauggccgu uuuaggagau acugcauggg auuuuggaag cguaggagga     1920

gcauuaaacu cauuaggaaa aggaauacau caaauauuug gagcagcauu uaaaagucua     1980

uuuggaggaa ugucaugguu uucacaaaua cuaauaggaa cucuguuaau gugguuagga     2040

cuaaauacaa aaaauggauc uauaagccuu augugccuag cucuaggagg aguuuuaaua     2100

uucuuaagua cagcuguauc agcagauguu ggauguucug uagacuucuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9211
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 3 opt

<400>  9211
gggagaaagc uuaccaugag aauuauaaau gcacgaaaag aaaagaaaag aagaggcgcu       60

gauacaagcg ugggaauugu gggauuauua uuaacaacag caauggcagc ggaaguuaca      120

agaagaggaa gugccuauua uauguaucua gauagaaaug acgcaggaga ggcaaucuca      180

uuuccaacua cacuuggaau gaauaaaugu uacauccaaa uaauggacuu aggacauaug      240

ugcgaugcaa cuaugucaua cgaauguccg auguuagaug aaggaguaga gccagacgau      300

guagauugcu gguguaauac aacuucaaca uggguaguau acggcacgug ucaucauaag      360

aaaggcgaag cuagacgauc caggagagca guaacauuac cuucacauag uacaagaaaa      420

uuacagacaa gaucacaaac augguuagaa ucuagagaau auacaaaaca ucuaauuaga      480

guugaaaacu ggauauuuag aaacccagga uucgcacuag cagcugcagc aauagcaugg      540

uuacuaggca gcucuacgag acaaaaagua auauaccuug uuaugauacu ccuaauagca      600

ccagccuauu caauuagaug cauaggagua aguaacagag auuuuguuga aggaaugagc      660

ggagguacau ggguagaugu aguauuagaa cauggaggau gugugacagu aauggcacag      720

gauaaaccaa cuguagacau agaauuagua acaacaacug uaagcaauau ggcagaagua      780

agaaguuacu guuaugaagc cucaaucagc gauauggcaa gugauucaag auguccaaca      840

caaggugagg cuuauuuaga uaaacaaagc gacacacaau auguaugcaa aaggacauua      900

guagacagag gauggggaaa cgguugcggc cuauucggaa aaggaagucu aguuacaugu      960

gcaaaauuug cuuguucuaa gaaaaugaca ggaaaaagca uacagcccga aaaccuagaa     1020

uaucgcauaa uguuaagcgu acauggauca caacauagug gaaugauagu uaaugauaca     1080

ggacacgaaa cagaugaaaa cagagcaaaa guggaaauaa cuccaaauuc acccagagca     1140

gaagcaacac uaggaggauu cggaucacua ggacuagauu gugaaccgag gaccggauug     1200

gauuuuagug auuuguacua uuugacuaug aauaacaaac auuggcuagu acacaaagaa     1260

ugguuucaug auauaccauu accauggcau gcaggagcag auacggguac accacauugg     1320

aauaacaaag aagcauuagu agaauuuaaa gacgcacaug caaaacgaca aacaguagua     1380

guacuuggga gucaggaagg agcaguacau acagcauuag caggagcauu agaagcagaa     1440

auggauggug caaaagguag acuuucaucc ggacaccuga aaugucgacu aaaaauggau     1500

aaauuaagau uaaaaggggu gagcuacagc uuguguacug cagcauuuac uuuuacuaaa     1560

auaccagcug aaacacuuca uggaacagua acuguagaag ugcaguaugc aggaacagau     1620

gguccaugua aaguaccagc acagauggcu guagauaugc aaacucuaac accuguuggc     1680

aggcuaauaa cugcaaaucc aguaauaaca gaaucaacag aaaacucaaa aaugauguug     1740

gaauuagauc caccauuugg agauaguuac auaguaauag guguugggga aaagaaaauu     1800

acucaucauu ggcacagauc aggaucuacg auaggaaaag cauuugaagc uacuguaaga     1860

ggcgcaaaaa ggauggcagu auuaggugau acggcauggg acuuuggauc uguaggugga     1920

gcguugaaua gucuagguaa aggaauacau caaauauuug gagcagcauu uaaaucauua     1980

uuugguggca ugaguugguu uagucagaua cuuauaggaa cucuauuaau gugguuagga     2040

uuaaacacca aaaauggauc aauaucacuu auguguuuag cauuaggggg aguccuaauc     2100

uuccuaucaa cagcaguaag cgcggaugua ggauguucag uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9212
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 3 opt

<400>  9212
gggagaaagc uuaccaugcg uauuauaaau gcaagaaaag aaaagaaaag aagaucagca       60

gauacaucag uugguauugu gggcuuacuu cucacaacag cgauggcagc cgaaguaacu      120

agaagaggau cagcuuacua cauguauuua gacaggaaug acgcugggga agcuauauca      180

uuuccuacaa cauuaggaau gaauaaaugc uauauacaaa uaauggaccu uggacacaug      240

ugugaugcaa ccaugagcua ugaaugucca auguuagacg aaggaguaga gccagaugau      300

guagacuguu ggugcaauac uacuuccacc ugggugguau auggaacaug ccaccauaag      360

aaaggugaag caagaagaag uagacgagca guaacacugc caucacacag uacaagaaaa      420

cuucaaacaa gaagccaaac auggcuagaa aguagagaau auacaaaaca uuuaauaaga      480

guagaaaacu ggauauuuag aaauccugga uuugcuuuag cugcugcagc aauagcaugg      540

cuauugggaa gcaguacaag ccaaaaaguc auauaccuag uaaugauacu ucuuauagca      600

ccagcauauu caauaagaug uauaggagua ucaaauagag auuuugucga aggcaugagu      660

ggaggaacau ggguagaugu uguuuuagaa cauggaggau guguaacagu aauggcacaa      720

gauaaaccaa caguagacau agaauuagua acaacaacag ucucaaacau ggcagaagua      780

agaucauauu guuacgaagc aucaauuuca gauauggcuu cagauucaag auguccuaca      840

caaggagaag ccuaccuaga uaaacaaagu gacacucaau acguaugcaa aagaacucua      900

guagauagag guuggggaaa cggauguggg uuauuuggaa aaggaagccu cguaacaugu      960

gcaaaauucg ccugcaguaa gaaaaugaca ggaaaaagca uacaaccaga aaacuuagaa     1020

uauagaauaa ugcuuagugu acacgguucu caacauucag gaaugauagu aaacgauaca     1080

ggccaugaaa cagaugaaaa cagagcgaaa guagaaauaa caccaaauuc acccagagca     1140

gaagccacac uuggaggauu uggcucauua ggauuagauu gugaaccacg aacaggacua     1200

gacuuuagug aucuauauua cuuaaccaug aauaacaaac acuggcuagu acauaaagag     1260

ugguuucacg auauuccacu cccauggcac gcaggagcag auacuggaac accgcauugg     1320

aauaauaaag aagcccucgu agaauuuaaa gaugcacaug cuaaaaggca aacgguggua     1380

guauuaggaa gucaagaagg agccguacac acagcauugg caggggcacu agaggcugaa     1440

auggacggag caaaaggucg auuaucaagu ggacauuuaa aauguagauu aaaaauggau     1500

aaauuaaggc uaaaaggagu aucguauuca uuaugcacag cagcauuuac auuuacaaaa     1560

auuccggcgg aaacacuaca uggaacagua acaguagaag uacaauaugc gggaacagac     1620

gguccaugua aaguaccugc acaaauggca guagauacac aaacuuugac accaguagga     1680

agacuaauua cagcaaaucc aguaauaacu gaaucaacag aaaauucaaa aaugauguua     1740

gaauuagauc caccuuucgg agauaguuac auaguaauug gaguggggga aaagaaaaua     1800

acccaccacu ggcaccgaag cgguucaacu auaggaaaag cauuugaggc aacaguuaga     1860

ggcgcaaaaa gaauggcggu auuaggagac acagcauggg auuucggauc uguaggaggg     1920

gccuuaaacu cacuaggaaa aggaauacau caaauauuug gagcagcguu uaaaucacua     1980

uuugguggga ugucuugguu uagccaaaua cuuauaggca cgcuacuaau guggcucggu     2040

uuaaauacca aaaauggauc uauaaguuua augugucuag cauuaggagg aguacuaaua     2100

uuuuugagua cagcaguguc agcugaugua ggcuguucag uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9213
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 3 opt

<400>  9213
gggagaaagc uuaccaugag aauuauaaau gcaagaaaag aaaaaaaaag acgaggagca       60

gacacuaaug uagguaucgu aggacuauua uuaacgacag ccauggcagc ugaaguaacc      120

cgaaggggau cggccuauua cauguauuua gauagaaaug augcaggaga agcaauaagu      180

uuuccaacaa cguuagguau gaauaaaugu uacauacaaa uaauggauuu aggucauaug      240

ugugacgcaa caaugaguua cgaaugccca auguuagaug aaggugugga acccgaugac      300

guugauugcu ggugcaauac aaccucaaca uggguugucu augggacaug ucaucauaaa      360

aaaggagaag caagaagaag uagaagggcc guuacucuuc caagccauag cacaagaaaa      420

cuccaaacua gaucacaaac auggcuagaa aguagagaau acaccaaaca cuuaauaaga      480

guagaaaauu ggauauuuag aaauccagga uuugcauuag cagcagcagc uauagcaugg      540

cuauuaggau cgucuacaag ucaaaaagua auauauuuag uuaugauacu acuaauagca      600

ccagcauaca gcauaaggug cauaggcgua ucaaacagag auuuuguuga aggaauguca      660

ggaggaaccu ggguagaugu aguguuagaa cauggagggu gcgugacagu aauggcccaa      720

gauaaaccaa caguugauau agaacuuguc acgacaacag ugucaaacau ggccgaagua      780

agauccuauu guuacgaagc aucaauaucg gauauggcau ccgauagcag auguccuaca      840

caaggagaag cauauuuaga caaacaaagc gauacacaau auguauguaa acgaacauua      900

guugacagag ggugggguaa ugggugugga cuauucggua aagguaguuu agucacuugc      960

gcaaaauuug cauguucuaa gaaaaugaca ggaaaaucaa uacaaccgga aaaccuagaa     1020

uacagaauaa ugcuaucagu gcauggauca caacauagcg gaaugauagu aaaugacacu     1080

ggucacgaga cagaugaaaa uagagcaaaa guagaaauaa caccaaauuc accaagagcg     1140

gaagcaacau uaggagguuu uggaucauug ggauuagauu gcgaaccaag aacaggauua     1200

gauuuuucag aucuuuauua ucugacaaug aauaauaaac auuggcuagu gcacaaagaa     1260

ugguuccaug auauaccccu accauggcau gcaggugccg auaccggaac accacauugg     1320

aauaauaaag aagcauuagu ugaauuuaaa gaugcucacg ccaaaagaca aacaguagua     1380

guauuaggcu cacaagaagg ggcaguacau accgcauugg cuggagcguu agaagccgaa     1440

auggacggug ccaaaggaag acucucaagu ggucauuuga aaugcagacu aaaaauggau     1500

aaauuaagau uaaaaggagu aucauauagu uuaugcacag cugcauuuac auuuacaaaa     1560

auaccagcag aaacucuaca uggaacaguu acaguugagg uucaauaugc aggaacagau     1620

ggaccaugca aagucccugc acaaauggca guagacaugc aaacacugac accuguagga     1680

agauuaauaa cugcaaaucc gguaauaaca gaaaguaccg aaaacucgaa aaugauguua     1740

gaacuugauc cuccguucgg agacagcuac auuguaauag gaguaggaga aaaaaaaaua     1800

acgcaucacu ggcauagauc aggaucgacc aucggaaaag cauuugaggc cacugugagg     1860

ggagcaagaa gaauggcagu auuaggcgau acagcuuggg auuuugguuc agucggagga     1920

gcauugaaua guuugggaaa aggaauacau caaauauuug gagcagcauu uaaaucauua     1980

uuugguggua ugagcugguu cucccagaua uuaauaggaa cacuauuaau gugguuaggu     2040

uuaaauacua aaaauggaag uauaucauua auguguuuag cuuuaggagg cguacuaaua     2100

uuuuuaucaa cggcuguuuc agcagaugug ggaugcucag uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9214
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 3 opt

<400>  9214
gggagaaagc uuaccaugag gauaauuaau gcuagaaaag aaaagaaaag aagaggagca       60

gaaacaagug uaggaaucgu ugguuuauua uuaacaacug cuauggcagc cgaaguaaca      120

aggagaggga gugccuauua cauguauuua gacagaaaug acgcaggaga agcaauuucc      180

uucccaacua cuuuagguau gaacaaaugu uauauacaaa uaauggauuu gggacauaug      240

ugcgaugcga caaugaguua ugaaugucca augcuagacg aaggagucga accugaugac      300

guagacuguu gguguaacac cacaaguaca uggguaguau auggaacgug ccaucauaag      360

aaaggagaag cgagaagaag uaggagagcc guaacauuac caagucacag cacaagaaaa      420

uuacaaacaa gaagucaaac augguuagaa ucaagagaau auacaaaaca ccuaauaagg      480

guagaaaacu ggauauuuag aaauccagga uuugcauugg cagcagcagc cauagcaugg      540

uuguuaggau caaguacaag ucaaaaagua auauauuuag uaaugauauu auuaauagca      600

ccagcauauu caauaagaug cauaggugua ucaaauagag acuuuguaga agguaugagu      660

ggaggaacuu ggguagaugu aguuuuagaa cacggaggau gcguaacagu aauggcucaa      720

gacaaaccaa caguagauau agaauuaguc acuacaacag uaucaaauau ggcagaaguc      780

agaagcuauu guuacgaagc aagcauuuca gacauggcau cugauucacg uuguccuacg      840

caaggagaag ccuauuuaga uaaacaaucc gacacacaau auguauguaa aagaacccua      900

guagacagag guugggguaa uggauguggc cuauucggga aaggaaguuu agucacaugu      960

gcuaaauuug caugcaguaa gaaaaugaca ggaaaaucaa uacaaccgga aaaucuagaa     1020

uacagaauaa ugcuaagugu acauggaagu caacauuccg gcaugauagu gaaugauacu     1080

gggcaugaaa ccgaugaaaa cagagcaaaa guagaaauaa caccaaauuc uccgcgugcu     1140

gaagcuacac uagggggauu uggaucauua ggucuugacu gugaaccaag gacugguuua     1200

gauuucucgg aucuauauua ucuaacuaug aauaacaaac auuggcuugu acauaaagaa     1260

ugguuucacg auauaccacu accauggcau gcaggagcag auacagggac uccucacugg     1320

aacaauaaag aagcauuagu ugaguuuaaa gaugcacaug caaaaagaca aacuguaguu     1380

guauuaggcu cacaagaagg ugcaguacac acagcucuag caggggcucu agaggcggaa     1440

auggauggug caaaaggaag acuuucaagu ggacacuuaa aaugcagacu aaaaauggau     1500

aaauugagau uaaaaggagu aaguuauucu uuauguacag cagcauucac auuuacaaaa     1560

auaccagcag aaacauuaca uggaacagua acaguugaag uacaauacgc gggaacagau     1620

gggccaugca aagucccagc gcaaauggcu guagacaugc aaacgcugac acccguaggg     1680

cgacuaauua cugcaaaucc aguaauaacu gaaaguacag aaaacaguaa aaugaugcug     1740

gaauuagauc ccccguuugg agacagcuau auaguaauag gaguagguga aaagaaaauc     1800

acacaucauu ggcauagauc agguucaaca auaggaaaag ccuuugaagc aacaguuaga     1860

ggagccaaac ggauggcagu gcuuggagau acggcuuggg acuucggauc aguaggaggc     1920

gcauuaaaca guuuagggaa aggaauacac caaauuuucg gugcagcuuu uaaaaguuua     1980

uuuggaggaa ugucaugguu uucucaaaua uuaauaggaa cuuugcuaau guggcuagga     2040

uuaaacacca aaaauggauc aauaucacug augugucuag cucuaggugg uguauuaaua     2100

uuucuaucaa cagcaguuuc agcagauguu ggaugcucag uugauuuuuc gaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9215
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 3 opt

<400>  9215
gggagaaagc uuaccaugag aauaauaaau gccagaaaag aaaagaaaag aagaggugca       60

gauacauccg uaggaauagu aggauuacuu uuaacaacag caauggcagc agaaguaaca      120

agaagggguu cagcauauua uauguaccua gauaggaaug acgcgggaga agcuauauca      180

uuucccacaa cccuaggaau gaauaaaugc uauauacaga ucauggaccu uggacauaug      240

ugugaugcaa caaugucaua cgaaugucca auguuagaug aaggaguaga accggaugac      300

gucgauugcu ggugcaacac uacgaguacc uggguagucu auggaacaug ucaucauaaa      360

aagggagaag caagacgaag uaggagagca gucacuuugc caagucacag uacuagaaaa      420

cugcagacaa gaagucaaac augguuagaa ucaagagaau auacuaagca ccuaauaaga      480

guugagaauu ggauauuuag aaacccaggu uuugcucuug cagcugcggc uauagcgugg      540

cuguuaggau caucaacaag ucaaaaaguu auauacuuag ugaugauauu auuaauugcu      600

ccugcauauu cacuaagaug uauuggagua aguaauagag auuuuguaga agguaugagu      660

ggaggcacgu ggguugaugu aguacuagaa cauggcggau guguuacagu aauggcacaa      720

gauaaaccua caguagauau agaauuagua acuacuacag uaaguaauau ggcagaaguu      780

agaaguuacu guuaugaagc aaguauauca gauauggcaa gugauucaag augucccacg      840

caaggagagg cuuaucuaga uaaacaauca gauacacaau acgucuguaa acgaaccuua      900

guagaucgag guuggggaaa ugggugcggu uuauucggaa aaggaucauu aguaacuugu      960

gcaaaauuug cauguucaaa gaaaaugaca ggaaaaagca uccaaccaga aaacuuagaa     1020

uauagaauaa uguuaagugu acaugguucu caacauucag gaaugauagu aaacgacaca     1080

ggccaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccaaauuc accaagagcu     1140

gaagcgacuu uaggaggauu ugguagcuua gguuuagauu gugaacccag aacaggauug     1200

gauuucagug auuuauauua ucuaacgaug aauaacaaac auugguuagu acauaaagaa     1260

ugguuccaug auauaccauu gccuuggcau gcuggagcag auacaggaac gccacacugg     1320

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg cgaaaagaca gacaguaguc     1380

guacuaggau cacaagaagg agcaguacau acagcauugg ccggagcuuu agaagccgaa     1440

auggauggag caaaaggcag auuaagcagu ggccacuuaa aaugucgauu aaaaauggau     1500

aaauuaagau uaaaaggagu caguuacagc uuauguacag cugcauucac auuuaccaaa     1560

aucccagcag aaacauuaca ugguacugug acaguagaag uacaauacgc uggaacagau     1620

ggcccuugua aaguaccagc acaaauggca guagauaugc aaacauuaac accgguagga     1680

agguuaauua cugcaaaucc aguaauaacu gaaucaacag aaaauaguaa aaugaugcua     1740

gaauuagacc cacccuuugg ggauaguuau auagugauag gaguaggaga aaagaaaaua     1800

acacaccacu ggcaucguag cggaaguaca auaggaaaag cauuugaagc uacgguaaga     1860

ggggcaaaac gaauggcugu gcuaggggac acagccuggg auuucggauc agugggaggu     1920

gcauuaaauu cauuaggaaa agggauacau caaauauucg gagcugcguu uaaaucauua     1980

uucggaggaa ugaguugguu uucucaaaua uuaauuggaa cgcuucuaau guggcuagga     2040

cuaaauacua aaaacgguuc aauaucucug auguguuuag cauugggagg aguacuaaua     2100

uuccuuagua cagcaguaag ugcggaugua ggcugcucag uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9216
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 3 opt

<400>  9216
gggagaaagc uuaccaugag aauuauaaac gcaagaaaag aaaaaaagag aagaggagcu       60

gacacuaaug uaggaauagu aggacuguua uuaacaacag cgauggcagc agaaguaacg      120

agaagaggau cugcauacua uauguauuua gauaggaaug augcaggaga agccauauca      180

uuuccgacaa cauugggaau gaauaaaugu uacauacaaa uaauggaucu aggacauaug      240

ugugacgcaa caaugaguua cgaguguccc auguuagaug aagggguuga gccagacgau      300

guggauuguu gguguaacac aacaaguacu uggguuguau auggaacuug ccaccauaaa      360

aaaggugaag cuagaagauc acguagggcg guaacacuac caucucacag uacccguaaa      420

cuacaaacga gaagccagac augguuagaa agcagagaau auacaaaaca uuuaauaaga      480

gucgaaaauu ggauguuuag aaauccaggc uuugcauuag cagcagccgc uauagcaugg      540

cuucuuggau caaguacauc acaaaaaguu auauauuuag uaaugauauu guuaauagcc      600

ccagcuuacu caauaagaug uauuggagua agcaacagag auuuuguaga aggaaugagu      660

ggaggaacau ggguugaugu aguacuagag cauggugggu gcguaacagu cauggcgcaa      720

gacaaaccua caguagauau agaauuagua acaacaacag uaaguaauau ggcagaagug      780

cgaucauauu guuacgaagc aagcauuucu gauauggcau cagauagucg uuguccaaca      840

caaggagaag cauaccuaga uaaacaauca gauacccaau acguauguaa aagaacuuua      900

guagaucgag guugggguaa uggaugugga cuauuuggua aaggaagccu aguuacuugu      960

gcuaaguuug caugcaguaa aaaaaugaca gguaaaucaa uacaaccaga aaaucuagag     1020

uauagaauaa uguuaagugu acauggaagu caacauagug guaugauugu aaaugauaca     1080

ggacacgaaa cagacgaaaa ccgagcuaaa guagaaauca cgccaaauag ucccagggca     1140

gaagcaacau uaggaggauu uggcucguua ggauuagacu gugaaccacg aacugggcua     1200

gauuuuagcg auuuauauua ucuaacaaug aacaacaaac acugguuagu acauaaagaa     1260

ugguuucaug auaucccacu accuuggcau gccggagcag acacaggaac accacacugg     1320

aauaauaaag aagcacuagu agaguuuaaa gacgcacaug cgaaaaggca aacaguagua     1380

guacuaggau cacaagaagg agcgguacau acggcacuag cuggagcccu agaagcagag     1440

auggacggcg cuaaaggaag auugagcagu ggacaccuaa aaugucguuu gaaaauggau     1500

aaauuaagac ucaaaggagu aaguuauucg uuaugcacag cugcguuuac auuuaccaaa     1560

auaccagcag aaacgcuaca uggaaccgua acgguggaag uucaauaugc aggaacagau     1620

ggaccaugca aaguaccagc ucagauggcg guagacaugc aaacauuaac accaguaggg     1680

agguuaauaa cagcaaaccc ugugauaacu gaaaguacag aaaauaguaa gaugaugcua     1740

gaacuagauc caccauucgg agauucauau auaguaauag gaguagggga aaaaaaaaua     1800

acucaucauu ggcauagaag ugguaguaca aucggaaaag cauuugaagc aacaguacgc     1860

ggggcaaaaa gaauggcagu acuuggagau acagcauggg auuuuggaag uguaggaggu     1920

gcguuaaaua guuuaggaaa aggaauacau caaauauucg gggcagcauu caaaaguuua     1980

uuuggaggaa ugaguugguu cagucaaaua uuaauaggaa cauuguuaau guggcuaggu     2040

cuuaauacaa aaaaugguuc aauaaguuua augugucuug cauuaggagg agucuuaaua     2100

uuuuuaagca cagcaguaag ugcggacguu ggguguucag uugacuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9217
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 3 opt

<400>  9217
gggagaaagc uuaccaugag aauaauaaau gcacgcaaag agaaaaaaag aagaggagca       60

gauacaagug uaggaauagu ugguuuauua cuaacaacag caauggcagc ugaagucaca      120

agaagaggau cagcauauua uauguaccua gacagaaacg augcuggaga agcgauauca      180

uuuccuacga cacuugguau gaauaaaugc uauauucaaa uaauggacuu gggacauaug      240

ugugaugcaa ccaugucaua cgaaugcccu auguuagaug aggguguaga accugacgau      300

guagauugcu gguguaacac aacuaguacu uggguaguuu auggaacaug ucaccacaaa      360

aagggagaag caagaagauc aagaagggcc guaacucucc caucacacag uacaagaaaa      420

uuacaaacaa gaucacaaac gugguuagaa ucaagagaau auacaaaaca cuuaauaaga      480

guugaaaauu ggauacuacg uaauccagga uucgcacucg cagcugcggc uauagcaugg      540

cucuuagguu cgaguaccuc ucagaaggua auauacuuag uaaugauauu auuaauagca      600

ccagcuuauu cuauaagaug uauaggaguc aguaauagag auuuuguuga aggaauguca      660

ggaggaacuu ggguagacgu gguacuggaa cacggaggau gcgugaccgu uauggcgcaa      720

gauaaaccaa cgguagauau agaauuagua acaaccacag uuaguaauau ggccgaggua      780

aggucuuauu guuaugaagc cucuauaucu gacauggcau cagacucaag augcccuaca      840

caaggagaag cauauuuaga uaaacaauca gauacacaau auguauguaa acgaacacua      900

guagauaggg gcugggguaa uggaugcgga cuauucggaa aaggaaguuu agucacaugu      960

gcaaaauucg cauguucuaa aaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa     1020

uaucgaauua uguuaucagu acauggauca caacauucug gaaugauagu aaacgacaca     1080

ggccacgaaa cagacgaaaa uagagcaaaa guugaaauaa caccaaauag uccucgagcc     1140

gaagcaacac uuggagguuu uggaagucua ggauuagauu gugaaccaag aacaggauua     1200

gacuucucgg aucuauauua ucugaccaug aauaauaaac auugguuagu gcacaaagaa     1260

ugguuucaug auauaccguu accauggcau gccggagcag auacaggcac accacauugg     1320

aauaauaaag aagcacuagu agaauucaaa gaugcucacg cuaaaagaca aacaguggua     1380

guauuaggua gccaagaagg agcaguacac acagcccuag caggugcucu ugaagcagaa     1440

auggauggag caaagggaag auuaaguucc ggacaccuga aaugcagacu aaaaauggau     1500

aaauuaagac uaaaaggagu cucauauuca uugugcacag cagcauuuac uuuuacaaaa     1560

auuccagcag aaacguugca cggaacagua acuguagaag uacaauacgc aggaacagau     1620

ggaccgugua aaguaccagc acaaauggca guugauaugc aaaccuuaac accaguugga     1680

agauuaauaa cugcaaaccc aguaauaaca gaaaguacgg aaaauucaaa aaugauguua     1740

gaauuagauc cuccauucgg agauucauac aucguaauag gaauaggaga aaaaaaaaua     1800

acacaucauu ggcacagauc gggaucaaca auagguaaag cauuugaagc uacaguaaga     1860

ggagcaaaac ggauggcagu auuaggagac acugccuggg auuuuggauc aguaggggga     1920

gcauuaaauu cacuaggaaa aggcauccau caaauauuug gugcugcuuu uaaaucccua     1980

uucggaggaa ugagcugguu cucacaaaua cuuauuggaa cucugcuaau guggcuagga     2040

uuaaauacaa aaaauggaag cauaagcuua augugucuag cucuaggagg cguucuaauu     2100

uuuuuaagua cagccguauc agcugacgua ggauguucag ucgauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9218
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 3 opt

<400>  9218
gggagaaagc uuaccaugag aauaauuaac gcuagaaaag aaaagaaaag aagaggagcu       60

gacacaagcg ugggaauagu aggacucuug cuaacaacag caauggcugc agaaguaaca      120

agaagaggaa gugcauauua uauguauuug gaucgaaaug acgcaggaga agcaauauca      180

uuuccaacaa cacucggaau gaauaaaugc uauauacaaa uaauggaucu cggacauaug      240

ugcgacgcca caaugucuua ugaauguccu augcuagaug aaggaguaga accagacgau      300

gucgauuguu gguguaauac aacaagcaca ugggugguau acggaacaug ucaucauaag      360

aaaggugaag caaggagaag uagacgggca guaacacuac caucacauag uacgcgaaaa      420

cuacaaacaa gaucacaaac uuggcuagaa ucaagagaau acacaaaaca ucuaauuaga      480

guagaaaacu ggauauuuag aaacccaggc uucgcacugg cagcagcagc aauagcaugg      540

uugcuagggu caagcacauc acaaaaaguu auauauuuag uaaugauauu auugauagcu      600

ccagcauaca guauaagaug uauaggagug ucaaaucgag auuuuguuga aggaauguca      660

ggaggcacuu ggguagaugu aguauuagaa cauggugguu gcguaacagu aauggcucaa      720

gauaaaccaa caguagauau cgaauuagua ucaacaacag uuucaaacau ggcagaagua      780

agaucuuauu guuacgaagc aaccauaagc gauauagcaa gugauucaag guguccuaca      840

caaggagaag cauaucuaga caaacaauca gauacacaau acguaugcaa aagaacauua      900

guugauagag gauggggaaa uggaugcggg uuauuuggaa aagggaguuu aguaacuugu      960

gcaaaauuug cguguucaaa gaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa     1020

uaucgaauua uguugucagu acauggauca caacauagcg ggaugauagu caaugauaca     1080

ggacaugaaa cugacgaaaa uagagcaaaa guagaaauaa caccaaauag cccuagagcc     1140

gaagcuacau uaggaggauu ugguucacuc ggacuagacu gugaaccaag gacuggucuu     1200

gauuuuucag auuuauauua cuuaacaaug aauaauaaac acugguuagu ccacaaagaa     1260

ugguuucaug acauaccauu gccuuggcau gcaggagcag auaccggaac accacacugg     1320

aauaauaaag aagcauuggu agaauuuaaa gacgcucaug caaaaaggca aacagcuguu     1380

guacucggau cucaagaagg agcaguucac acagcuuuag cuggagcacu ugaagcagaa     1440

auggauggag caaaaggaag acuaucuucg ggccauuuaa aaugcagacu aaaaauggau     1500

aaauugcgau uaaaaggggu uaguuacagu uuauguacag cggcuuuuac uuuuacaaaa     1560

auaccagcag aaacacuaca uggaacagua acaguagaag uacaauaugc ggguacagau     1620

ggcccaugca aaguaccagc acaaauggca guagauaugc aaacacuaac gccaguagga     1680

aggcugauua cagcaaaucc aguaauaaca gaaucuacug aaaacucaaa aaugaugcuc     1740

gaacuagauc caccauuugg ggauucuuau aucguuauag gcguaggcga aaagaaaauu     1800

acacaccauu ggcacagauc aggaucaaca auaggaaaag ccuuugaagc uacaguaaga     1860

ggugcaaaaa gaauggcugu ucuuggagac acagcauggg auuuugguag uguaggggga     1920

gcauugaacu cacuagggaa aggcauacau caaauuuuug gugcagcauu uaaaucauua     1980

uucggaggaa ugagcugguu uagccaaauu cuaauuggaa cuuuacugau guggcuaggc     2040

cuuaauacua aaaauggaag uauaucacuu augugcuuag cauuaggagg aguacugaua     2100

uuuuuaucaa cagccguuuc agcagacguu gguugcagug uugauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9219
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 3 opt

<400>  9219
gggagaaagc uuaccaugag aauaauaaac gcucguaaag aaaaaaagag aagaggagca       60

gacacaagug uaggcauagu aggauugcuu uugacuacag caauggcagc agagguaaca      120

aggagagggu cagcuuauua uauguacuua gacagaaacg acgcagguga agccauaucc      180

uuuccgacaa cacuagguau gaauaaaugc uacauacaaa ucauggauuu aggacauaug      240

ugcgaugcaa caaugagcua cgaaugucca auguuagaug aaggaguaga accagaugau      300

guugauuguu gguguaacac aacuagcaca uggguagucu auggcacaug ucaccacaaa      360

aaaggcgaag cuagaagauc cagacgagcc guaacauuac cuagucauag cacgagaaag      420

cuacaaacaa gaagccaaac augguuagaa aguagagaau auacaaaaca ucuaaucaga      480

guggaaaacu ggauauucag aaaccccggu uuugcauuag cugcagcagc cauagcaugg      540

cuauuaggaa guucaacgag ccaaaaagua auauauuuag uaaugauauu acugaucgcu      600

ccagcauauu caauacgaug uauaggggua agcaauagag acuucgucga aggaauguca      660

ggaggaacau ggguagaugu ugugcucgaa cacggugguu gugucacagu aauggcacaa      720

gauaaaccaa ccgucgauau agaacuagua acaacaacag uuucaaauau ggcagaagua      780

agaucuuacu guuaugaggc aaguauaagu gauauggcgu cagauucaag augcccaacu      840

caaggagaag cauauuuaga uaaacaaagu gauacacaau acguauguaa aagaacuuua      900

guagauagag gauggggaaa uggcuguggu cuauuuggca aaggaagucu aguaacuugu      960

gcaaaauucg caugcagcaa aaaaaugaca ggaaaaagca uacaacccga aaacuuagaa     1020

uaucgaauaa ugcugucagu ccauggaagu caacacagug guaugauagu uaaugauacu     1080

ggacaugaaa cagacgaaaa uagagcgaaa guagagauaa cuccaaauuc gcccagagca     1140

gaagcuaccc uaggagguuu uggaucacuc gguuuggauu gcgaaccucg aacagguuua     1200

gacuuuagcg acuuauauua ccuaacaaug aacaauaaac auuggcuagu acacaaagaa     1260

ugguuucaug auauaccuuu accauggcau gcuggcgcag auacaggaac accgcauugg     1320

aauaauaaag aagcacuagu agaauuuaaa gaugcacaug ccaaaagaca aacugucguu     1380

guauuaggua cacaagaagg agcuguacau acagcauuag ccggagcauu agaagcagag     1440

auggacggug caaaaggccg auuaaguuca gggcaucuaa aauguagauu gaaaauggac     1500

aaauuacgac uaaaaggagu uucauauuca uuauguaccg cagcauucac uuuuacaaaa     1560

auaccugcag agacauuaca ugguacagua acaguagaag uacaauaugc aggaacagac     1620

ggaccaugua aaguaccugc ucaaauggca guagauaugc agacucuaac accagucggc     1680

agauuaauaa cagcaaaucc cguaauaaca gaaucgacgg aaaauucuaa aaugauguua     1740

gaacucgauc cuccauuugg agauaguuac auaguaauug ggguaggaga aaaaaaaauc     1800

acucaucauu ggcauagaag uggaucgacg auaggaaaag cauucgaagc aacaguacga     1860

ggagcuaaaa gaauggcugu uuuaggugac acugcauggg acuuuggauc aguuggagga     1920

gcguuaaaua gcuuaggaaa gggaauacau cagauauuug gagcagcguu caaaagucuc     1980

uucgguggaa ugucaugguu cagucaaaua cugauaggua ccuuauuaau guggcuagga     2040

uuaaauacaa aaaacgguuc cauuagucuu auguguuuag cauuaggcgg aguauuaaua     2100

uucuuaucaa cagcaguauc ggcagacgua ggauguagug uagauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9220
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 3 opt

<400>  9220
gggagaaagc uuaccaugag aauaauaaau gcccguaaag aaaagaaaag aagaggugca       60

gauacaucag uggguaucgu agguuuauua uuaacaacag cgauggcagc ggaaguaaca      120

agaagaggcu cagcauauua uauguaccua gacagaaacg augcgggaga agcaauaucu      180

uuuccuacaa cacuuggaau gaauaaaugc uauauacaaa uuauggacuu aggacacaug      240

ugugaugcua caaugagcua cgaaugucca auguuagaug aaggaguaga accagaugac      300

gucgauugcu ggugcaacac uacgagcaca uggguuguuu acgguacaug ccaccauaag      360

aaaggagaag cacgaagauc aagaagggcu gucacguuac cgucacauuc aacaagaaaa      420

uugcaaacaa gaucacaaac augguuagag ucaagagaau auacaaaaca uuuaauuaga      480

guagaaaauu ggauuuuuag aaauccaggu uuugcacuug cagcagcugc uauagcaugg      540

uuauuaggau caagcacauc acaaaaaguc auauauuuag ugaugauuuu acuaauugca      600

cccgcauaca gcauaagaug uauaggagua ucaaauagag acuucguaga aggaaugucg      660

ggaggaacuu ggguagaugu aguauuagaa caugggggau guguaacagu aauggcucag      720

gauaaaccaa cuguugauau ugaauuggua acaacaacag ugaguaauau ggcugaagua      780

agaaguuauu guuacgaagc aaguauaagu gauauggcaa gugauucaag guguccuacu      840

caaggagagg cauacuuaga caaacaaucu gacacucaau auguauguaa acgaacauua      900

gucgaucgag guugggguaa uggauguggu cuauuuggaa aaggaucgcu aguaacaugu      960

gccaaauuug cauguaguaa gaaaaugaca ggaaaaagua uucaaccaga gaacuuagaa     1020

uaucgaauua uguuaucagu ucauggaagu caacauagug gaaugauagu aaaugacacc     1080

ggucaugaaa cggaugaaaa uagagcaaaa guugagauaa caccaaauag uccuagagca     1140

gaagcaacac uaggagguuu cggaagucua gguuuagauu gugaaccacg aacaggauua     1200

gacuuuagcg aucuauacua cuuaacuaug aacaauaaac acugguuggu acacaaagaa     1260

ugguuucaug acauaccacu accguggcac gcuggagcug auacuggaac accacauugg     1320

aauaauaaag aagcauuggu agaguuuaaa gaugcacacg caaaaagaca aacaguagua     1380

guauuaggaa gccaagaagg agcaguacau accgcauugg cuggugcauu agaggcagaa     1440

auggauggug cuaaagguag guuaaguuca ggccauuuaa aauguagauu aaaaauggau     1500

aaauuaagac uaaaaggugu cucauacagc uugugcacag cagcauucac auuuacaaaa     1560

aucccugcag aaacauugca uggaacggua accguagaau uacaauaugc aggcacagau     1620

ggaccuugua aaguaccagc ucaaauggca guagauaugc aaacuuuaac uccaguagga     1680

agauuaauaa cugcaaaucc agugauaacg gaaaguacug aaaacaguaa aaugauguua     1740

gaacuugauc cgccauuugg agacagcuac auagugauag gugugggaga aaagaaaaua     1800

acccaucauu ggcaccgauc aggaucaaca auaggaaagg cuuucgaagc aacaguuaga     1860

ggcgcaaaac gcauggcugu auugggagau acugcauggg auuuuggaag uguagguggg     1920

gcauuaaaca guuuaggaaa aggaauacau caaauuuucg gcgcagcauu uaaaucauua     1980

uucggaggaa ugagcugguu uucacaaaua cuuauaggaa cacuacuaau gugguuaggu     2040

uuaaauacua aaaacggaag uauuucauua auguguuuag cacucggagg uguauuaaua     2100

uuccuaucaa cagcaguauc agcagacgua ggaugcagcg uagacuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9221
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 3 opt

<400>  9221
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaagaaaag aagaggagca       60

gaaacaagug uaggaaucgu uggauuauua uuaacaacag caauggcagc agaaguaacg      120

agacggggau cagcuuauua cauguauuug gauagaaaug augcaggaga agcuauuagc      180

uuuccaacaa cacuggguau gaauaaaugu uauauacaaa uaauggacuu gggucauaug      240

ugcgacgcaa caaugucaua ugaauguccc auguuagaug aaggaguaga accagacgau      300

guagauuguu gguguaauac aacaaguaca uggguaguau augguacgug ccaucauaaa      360

aagggagaag caaggagauc aagaagggca guaacauuac cuagccauag cacucgaaaa      420

uuacaaacaa gaagucagac gugguuggaa ucaagagagu auacaaaaca cuuaauacgg      480

auagaaaauu ggauauucag aaacccaggu uucgcucugg cugccgcagc aauugccugg      540

cuauuagggu caagcaccuc acagaaagug auuuacuuag uaaugauacu auuaauagcu      600

ccugcauaca gcauuagaug uauaggcguu ucaaaccgag acuuugucga aggaaugucg      660

ggagguacau gggucgaugu aguauuagag cacggaggau gugugacagu aauggcacaa      720

gacaaaccaa cagucgacau agagcuagua acaacaacug uaaguaauau ggcagaaguu      780

agaucauauu guuaugaagc caguauaagu gauauggcua gugauuccag auguccaaca      840

caaggagaag cauaucuuga uaaacaaagc gauacacaau auguuuguaa aagaacuuua      900

guagauaggg gaugggguaa cgggugugga cuauuuggaa aaggaucuuu aguuacaugu      960

gcaaaauuug cauguaguaa gaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa     1020

uauagaauua uguugucagu acaugguuca caacauagug guaugauagu aaaugacacc     1080

gggcaugaaa cagacgaaaa uagagcaaaa guagaaauaa ccccaaauag cccgagagca     1140

gaggcaacau uggguggauu uggaagccuu ggacuagauu gugaaccaag aacagggcua     1200

gauuucagug accuguacua uuuaacaaug aacaacaaac auugguuagu ccauaaagag     1260

ugguuccaug auauccccuu accauggcac gcaggagcag acacgggaac accacacugg     1320

aacaauaaag aagcauuagu cgaauuuaaa gaugcacaug caaaaagaca aaccguagug     1380

guauuaggau cacaagaagg ugcaguucau acagcuuuag cuggagcacu ugaagcagaa     1440

auggauggcg caaaaggaag auuaucaagu ggacauuuaa aauguagauu aaaaauggau     1500

aaauugagac uaaaaggugu aucauacagu uuauguacag cagcauuuac uuuuacaaaa     1560

auaccugcag aaacauuaca cgguacagua acaguagaag uacaauaugc aggaacagac     1620

ggaccaugca aagucccggc acaaauggcu guagauaugc aaacacuaac accaguggga     1680

agauuaauua cagcaaaucc aguaauaaca gaaucaacug aaaauucgaa aaugaugcua     1740

gaauuggauc caccauuugg agacaguuau auaguaauug guguuggcga aaagaaaaua     1800

acacaucauu ggcauagauc aggaucaacu auaggaaaag cauuugaggc uacagugcga     1860

ggagcuaaac gaauggcagu guuaggugau acggcauggg auuuuggaag uguaggcggu     1920

gcccuaaauu cacuuggaaa aggcauacac caaaucuuug gagcagcauu caaaucgcua     1980

uuugguggaa ugaguugguu uagucaaaua uuaaucggga cuuuauuaau gugguuagga     2040

uuaaauacua aaaauggaag cauaucacua auguguuuag cguuaggagg aguauuaauu     2100

uucuuaagca cagccguauc agcagaugug ggaugcagug uagauuucuc gaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9222
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 3 opt

<400>  9222
gggagaaagc uuaccaugag aaucauaaau gcuagaaaag aaaaaaaaag acggggagca       60

gauacaucag uaggaauagu gggauuauua cuaacaacug caauggccgc agaaguuaca      120

cgaagaggga gugcauauua uauguaucua gaccgaaaug augcaggaga agcaauaagc      180

uuuccaacaa cccuuggaau gaauaaaugu uauauacaaa uaauggauuu aggacauaug      240

ugugacgcaa caaugucaua ugaaugcccg auguuagacg aagggguaga accagacgau      300

guagacugcu gguguaauac aacaucaaca uggguuguau acggaaccug ccaucauaaa      360

aaaggagaag caagaagaag uagaagggcg guaaccuuac cuucucauuc aacuagaaaa      420

cuucaaacua gaucacaaac uugguuagaa aguagggaau auacaaaaca uuuaauaaga      480

guagagaauu ggauuuuuag aaauccagga uuugcauuag cagcagcagc aauagcaugg      540

uuacucgguu caucaacaag ucaaaaagua auuuacuuag uaaugauuuu acuaauugca      600

cccgccuacu caauuagaug uauaggagua ucaaauagag auuucguaga aggaaugagu      660

ggaggcacuu ggguugaugu aguauuagaa caugguggcu guguaacagu cauggcacaa      720

gauaagccaa caguagauau agaacuagua acaaccaccg uaucaaacau ggccgaagua      780

cgaucguacu guuaugaagc aucaauuagu gacauggcau cagauaguag augucccaca      840

caaggagaag cauaucuaga uaaacaaagu gauacucaau auguauguaa aagaacauua      900

guagaucgag guugggguaa uggaugugga cuauuuggaa aaggaucucu agucaccugu      960

gcaaaauuug cuuguucaaa aaaaaugaca ggaaaaagca uucaaccaga aaauuuagaa     1020

uauagaauua uguuaucagu acauggaagc caacauucug gaaugauagu aaaugauacu     1080

ggacacgaaa cagaugagaa uagggcaaaa guugaaauaa caccaaauuc accuagagca     1140

gaagcaacau uaggcgguuu ugguagcuug gguuuggauu gugaaccuag aacaggacua     1200

gauuuuagug auuuauauua ucuaacaaug aauaauaaac auuggcuggu acauaaagaa     1260

ugguuucaug auauaccauu accuuggcau gcaggagccg auaccgguac gccucauugg     1320

aacaauaaag aagcacuugu agaauucaaa gaugcacacg caaaacguca aacaguagua     1380

guauuaggau cacaagaagg ggcaguacau acggcguuag caggcgcauu agaagcagaa     1440

auggauggag cuaaaggucg ucuuucaagu ggacauuuaa aauguagguu aaaaauggau     1500

aaacuacgau uaaaaggcgu aucuuauuca uuauguacag cagcauuuac uuuuaccaaa     1560

auaccagcag agacccuaca uggaacaguc acgguagaag uacaauaugc ugguacugau     1620

ggaccaugua aaguaccugc ucaaauggcc guagauaugc aaacauuaac accaguggga     1680

agacuaauaa cagcaaaucc gguuauuaca gaaagcauag aaaauucaaa aaugauguua     1740

gaacuagauc cacccuucgg ugauucauau auaguuauag gcguagggga gaaaaaaaua     1800

acgcaucacu ggcaucgauc aggaaguaca auaggcaaag cauuugaagc uacuguacgu     1860

ggagcaaaac gaauggccgu ucucggggac acugcuuggg acuucggcuc aguaggaggg     1920

gcgcuaaaua gucucggaaa aggcauacac caaauuuuug gagcagcuuu caaaucauua     1980

uucggaggaa ugucgugguu cagucaaaua uuaaucggaa cacuacuaau guggcuagga     2040

cucaauacca aaaaugggag uauaaguuua augugucuag cauuaggagg uguuuuaaua     2100

uuuuuaucaa cugcuguuuc agcagacgua ggaugcucag uugauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9223
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 3 opt

<400>  9223
gggagaaagc uuaccaugag aauaauaaac gcaaggaaag aaaagaaaag aagaggagcu       60

gauacaucug uaggaauugu aggguuacua uuaacaacag caauggcugc ggagguaaca      120

aggaggggcu cagcauacua uauguaucua gaccgaaaug augcaggaga agcaauaagu      180

uucccaacaa cauugggaau gaauaaaugu uacauacaaa uaauggauuu aggccauaug      240

ugcgaugcaa caaugaguua ugaaugcccg augcuagaug aaggaguuga acccgaugau      300

gucgauuguu gguguaauac aacaaguacu uggguaguau acggaacaug ucaucauaag      360

aaaggugaag cuagaagauc aaggagagcg guaacacuac cgucacauuc aaccagaaaa      420

uuacaaacaa gaucacaaac cugguuagaa aguagagaau auacaaaaca uuuaauaaga      480

guagaaaacu ggauauuuag aaauccaggu uuugcauugg cugcugcagc uauagcaugg      540

uuacuaggaa gcucaacaag ucagaaagua auauaucuag ucaugauuuu acuaaucgca      600

ccagccuaca guauaaggug uauaggagua ucaaauagag acuuuguaga agguaugagu      660

ggagguacau ggguagacgu aguacuagaa cacggagguu guguaaccgu aauggcccaa      720

gauaaaccga caguagacau agagcuagua acaacaacag uuucaaauau ggcugaaguu      780

agaagcuauu gcuaugaagc cucaauaagc gacauggcua gugauucaag augucccaca      840

cagggagaag cauauuuaga uaaacaguca gacacucaau auguauguaa gagaacauua      900

guagacaggg guuggggaaa uggaugugga uuauucggaa aaggaagucu uguaacaugc      960

gcaaaauuug cauguucgaa gaaaaugaca ggaaaaagua uacaaccaga aaacuuagaa     1020

uauagaauaa ugcuuagugu acauggaagu caacauagug gaaugauagu uaaugauacu     1080

ggucaugaaa cagaugaaaa uagagcaaaa guagaaauaa cuccaaacag cccuagagca     1140

gaagcuacau uaggaggauu ugguaguuua ggacuagauu gcgagccuag aaccgggcug     1200

gacuuuucug auuuauauua cuuaacaaug aacaauaaac auuggcuagu acauaaagaa     1260

ugguuccaug auauaccuuu gccauggcac gcaggggcag auacaggaac accucauugg     1320

aauaacaaag aagcucuagu agaauuuaaa gaugcacaug caaaaagaca aacaguuguu     1380

guauuaggaa gucaagaagg agcaguacac acagcacuag caggagcauu agaagcagaa     1440

auggauggag uaaaaggccg gcuuagcagc ggucaucuaa aauguagauu aaaaauggac     1500

aaacuaaggu uaaaaggugu aaguuauagu cuguguacug cggcauuuac auuuacaaaa     1560

auaccagcag aaacauuaca ugguaccgua acaguagaag uacaauacgc aggaacggau     1620

ggacccugua aaguuccugc acaaauggca guagacaugc aaacacuaac accugugggu     1680

aggcuaauaa cugcaaaucc gguaauaaca gaaucaacug aaaauaguaa aaugaugcuu     1740

gaauuggauc ccccuuuugg agacaguuau auaguaauag ggguaggaga aaagaaaaua     1800

acucaccacu ggcauagaag cggaucaacu auagggaaag cguucgaagc aacaguacga     1860

ggcgcaaaaa gaauggcagu auuaggagac accgcauggg auuuugguag uguaggaggu     1920

gcacuuaaua gccuaggcaa aggcauacac caaauauucg gagcugcauu uaaaucauua     1980

uuuggaggga ugagcugguu cucacaaaua uuaaucggaa cauuauuaau guggcuugga     2040

cuaaauacca aaaauggauc aauaucauuu augugucuag cacuuggcgg aguauuaauu     2100

uuucuaagua cagcaguuuc agcagacgua ggcuguagug uagauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9224
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 3 opt

<400>  9224
gggagaaagc uuaccaugcg uauaauaaau gcacgaaaag aaaaaaaaag aagaggagcu       60

gacacaucag uuggaauugu aggauuauua uuaacuacag caauggcagc agaaguaaca      120

agacgaggau cugcuuauua uauguauuua gauagaaacg augcgggaga agcaauauca      180

uuucccacaa cauugggaau gaauaaaugu uacauacaaa uuauggauuu aggacauaug      240

ugugaugcaa caaugagcua ugaaugucca augcuagacg aaggaguuga accugaugau      300

guagacuguu ggugcaauac uacaucaaca ugggucguau auggaacaug ccaucacaaa      360

aaaggugaag cuagaagaag uagaagagca guaaccuuac caucccacag uacacgaaaa      420

cuacaaacaa ggagucaaac augguuagaa ucaagagaau auaccaaaca ucugauacga      480

guagaaaauu ggauauucag aaacccagga uucgccuugg cagcugccgc gauagcaugg      540

cuacuaggaa guucaacuuc acaaaaagua auauacuuag ucaugauacu acuuauugcg      600

ccagcauaua gcauacgaug uauaggaguc agcaauagag auuuugucga aggaaugucu      660

ggaggaacau ggguagacgu aguauuagaa cauggaggau guguaacagu aauggcacaa      720

gauaaaccaa cuguagauau ugaauuaguu accacaacag uaucaaauau ggcagaagua      780

agaucauacu guuaugaagc aaguauaagc gauauggcaa gugacucaag auguccaacc      840

caaggagaag cauacuuaga uaaacaaagc gauacacaau auguauguaa acgaacguua      900

guagacagag gauggggaaa uggauguggu cuauucggaa aagguaguuu agugacaugu      960

gcaaaauuug cuuguaguaa aaaaaugaca ggaaaaucca uucaaccaga aaaucuagaa     1020

uauagaauaa ugcugagugu acauggauca caacacucug guaugauagu caacgacacu     1080

ggacaugaaa cugaugaaaa uagggcuaaa guagaaauaa caccuaauuc accaagagcc     1140

gaagcuacuu uaggugguuu uggcucauua ggcuuagauu gugaaccacg aacaggauua     1200

gauuuuucag accuauacua ccuaacaaug aacaacaaac auugguuagu ucauaaagaa     1260

ugguuucacg auauaccacu accuuggcau gcaggugcag auacuggaac uccacauugg     1320

aauaacaaag aagcacuagu cgaauuuaaa gacgcacaug caaaacgaca aacaguagua     1380

guuuugggcu cucaagaagg ugcaguucau acagcacuag cuggugcucu agaagcagaa     1440

auggauggag cuaaggguaa acuaaguagc ggacauuuaa aaugcagacu aaaaauggau     1500

aaauuacgac uaaaaggagu aaguuauuca uuaugcacag cggcauucac cuuuacaaaa     1560

auaccagcag aaacauuaca cggaacaguu acaguugaag uccaauaugc aggaacagau     1620

ggaccaugua agguaccugc ccaaauggcu guagauaugc aaacacuuac accuguugga     1680

agacuuauua cagcuaaccc cguaauaacc gagucaacag aaaacaguaa aaugaugcua     1740

gaacuagacc ccccauuugg ugauaguuau auagugauag ggguagguga aaaaaaaaua     1800

acacaucauu ggcauaggag cggaucaaca auagguaaag cauuugaagc cacaguaaga     1860

ggugccaaaa gaauggcagu guuaggagac acagcauggg acuucggauc agugggcggu     1920

gcacuaaaca guuuagguaa gggaauacau cagauauuug gugcugcauu uaaaucuuua     1980

uuuggaggaa ugaguugguu uagucaaauu uuaauuggaa cacuauuaau guggcuaggg     2040

cuaaacacga aaaacggcuc aauaucauua augugcuuag cauuaggugg aguuuuaaua     2100

uuucuaucaa cagcaguuag ugcugacguu ggaugcuccg uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9225
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 3 opt

<400>  9225
gggagaaagc uuaccaugag aauaauaaac gcuagaaaag aaaagaaaag aagaggagcu       60

gauacaagug ucgguaucgu uggacucuua uuaacaacug caauggccgc agaagugaca      120

cgaaggggua gugcauauua cauguauuua gauagaaaug augcaggaga agcgauauca      180

uuccccacaa cacuaggaau gaauaaaugc uauauacaaa uuauggauuu aggucacaug      240

ugugacgcaa caaugucaua ugaaugucca auguuagacg aaggaguaga accagaugau      300

guagauugcu gguguaauac aacaucaaca ugggucguau acggaacuug ucaccacaag      360

aaaggagaag cuaggagaag uagacgagca guaacucuac cuagucauuc uaccagaaaa      420

cuccaaacca gaucacaaac augguuggaa ucaagagaau auacuaaaca ccugauaagg      480

guagaaaauu ggauauuucg uaacccaggu uucgcauuag cagcagcagc aaucgcaugg      540

uuauuaggaa gcagcacauc acaaaaagua auauauuuag ugaugauauu acuaauagcg      600

ccugcauaua guauuagaug uauaggagua ucaaaucgag acuuuguuga aggaauguca      660

ggaggaacau ggguagacgu aguucuugaa cacggaggau guguaacagu aauggcacaa      720

gacaaaccaa cgguagauau agaauuagua acaacgacag uaucaaacau ggcagaagua      780

agaucuuacu guuacgaagc guccauauca gauauggcaa gcgauucaag augcccaacc      840

caaggugaag cauaucuaga uaaacaaagu gauacacaau auguauguaa aaggacucua      900

gucgacagag ggugggguaa uggaugugga cuauuuggaa aaggaucauu aguaacaugu      960

gcaaaauuug cauguucaaa gaaaaugacc ggaaaaagca uccaaccaga aaaucuugaa     1020

uaccgaauua ugcuaucagu acauggauca caacacucag gaaugauagu aaaugacaca     1080

ggucaugaaa cagaugaaaa cagggcaaaa guagaaauaa caccaaauuc accuagagca     1140

gaagcaaccc ucggagguuu uggaucauua ggauuagauu gugaaccuag aacaggacua     1200

gauuuuucag acuuauacua cuuaaccaug aacaauaaac auugguuagu acacaaagaa     1260

ugguuucaug acauaccuuu gccauggcac gcaggagccg auaccggaac accacacugg     1320

aacaauaaag aggcuuuagu agaauuuaaa gaagcacaug caaaacgaca aacaguagua     1380

guauuaggaa gucaagaagg ggccguacac acagcacuag caggagcauu agaagcagaa     1440

auggauggag cuaaaggaag acuaucaagu ggacauuuaa aaugcagacu aaaaauggau     1500

aaacuaagac uaaaaggggu cagcuacagu cuauguacag ccgcauuuac auuuaccaaa     1560

auaccagcag aaacacuaca cggaacagua acaguagaag uacaauacgc aggaacugac     1620

ggaccaugua aaguacccgc acaaauggca guagauaugc agacauuaac uccagucggc     1680

agauuaauaa cugcaaaccc ugucauaaca gaaaguacag aaaauucaaa gaugauguua     1740

gaacuagauc cacccuucgg cgacagcuau aucguuauag gagugggaga aaagaaaaua     1800

acacaucauu ggcaucgauc agguaguaca auaggaaagg cuuuugaagc aacaguaaga     1860

ggugccaaaa gaauggcugu acuuggagau acagcauggg auuuuggauc aguagguggu     1920

gcuuuaaaua guuuaggaaa aggaauacau caaauuuuug gagcagcauu uaaaucauua     1980

uuuggaggaa ugucaugguu cucacaaaua uuaauaggaa cucuauuaau guggcucgga     2040

uuaaauacca aaaauggguc aauaaguuua augugccuag cauuaggggg uguauuaaua     2100

uuuuuaucaa cagcaguaag ugcagauguu ggauguucag ucgauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9226
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 3 opt

<400>  9226
gggagaaagc uuaccaugcg aauaauaaau gcuagaaaag aaaagaaaag aagaggagca       60

gauacuucag uuggaauagu agguuuacuu cucaccacug caauggcagc agaaguaaca      120

cguagaggcu cagcauacua cauguauuua gaucgaaaug augcaggaga agcaauauca      180

uucccaacaa cacucggaau gaauaaaugu uauauacaaa ucauggauuu gggacauaug      240

ugugaugcca cuaugagcua cgaauguccu augcuagacg aaggaguaga accugaugau      300

guagauuguu gguguaacac uacaagcaca uggguaguuu auggcacaug ucaucauaag      360

aaaggugaag caagaagauc aagacgagcg guuacacugc cuucucacuc uacaagaaaa      420

cuacaaacaa gaucacaaac uugguuagaa aguagagaau acacgaaaca uuuaauaaga      480

guagaaaacu ggauuuucag aaaucccgga uuugcuuuag cugcagcagc uauagcuugg      540

uuauuaggaa gcaguacaag ccaaaaagua auauauuuag uaaugauucu acuuauagcu      600

ccagcauauu caauaaggug uauaggaguu ucaaauagag auuuuguaga aggaauguca      660

gguggaacau ggguagaugu cguucuagaa cauggcggau gcguaacagu cauggcacaa      720

gauaaaccaa caguagauau ugaauuagua acaacaacug uaaguaauau ggcagaagua      780

agaucguauu gcuacgaagc aucaauaagu gauauggcau cagacucaag augcccaaca      840

caaggagaag cauaucuaga uaaacaauca gacacgcaau auguuuguaa gcgaaccuua      900

guagauagag guugggguaa cgggugcgga uuauuuggaa aaggcagucu aguaacaugc      960

gcuaaauuug cguguaguaa aaagaugacu ggaaaaucaa uucaaccaga aaaucuagaa     1020

uauagaauaa ugcugucagu acaugguuca caacauagug gaaugauagu aaaugacacg     1080

ggucacgaaa cagaugaaaa uagagcgaaa guagaaauaa caccaaauag cccacgagcu     1140

gaagcuacau ugggagguuu cgguucuuua gguuuagacu gcgaaccgag aacaggauua     1200

gacuucagcg aucuauauua uuuaacaaug aauaauaaac acugguuagu acauaaagaa     1260

ugguuucaug acauuccauu accauggcau gcaggagcag auacagguac accacauugg     1320

aacaauaaag aagcauuagu agaauuuaaa gaugcacaug caaaaagaca aacaguagua     1380

guuuuaggua gucaagaagg agcaguucac acagcacuag cuggugcauu agaagcugaa     1440

auggauggug cgaaaggaag acugaguucc ggacaccuaa aaugcagacu aaaaauggac     1500

aaacuaagac uaaaaggugu aucauacagc uuauguacag cagcauucac auucacaaaa     1560

auucccgcag aaacauuaca uggaacagua acaguugaag uccaguacgc gggugcagau     1620

gguccuugca aaguacccgc ccaaauggca gucgacaugc aaacuuuaac accuguagga     1680

agauugauaa cagcaaaucc aguaauaaca gaaucaacag aaaacaguaa aaugauguug     1740

gaacuugauc caccauucgg ugacaguuac auaguaauag ggguuggaga aaagaaaaua     1800

acacaucauu ggcauagaag uggaagcaca auaggaaaag cauuugaagc aacaguaaga     1860

ggcgcaaaac gcauggcagu acuuggagau acagcuuggg acuuugggag cguuggagga     1920

gcccuaaaca gucuuggaaa aggaauacau cagauauucg gagcugcauu caaaagucua     1980

uuuggaggaa ugucaugguu uagucaaaua uuaaucggua cauuauuaau guggcuaggc     2040

uuaaauacga aaaauggaag uauaagucuu augugucuag cauuaggugg uguacuaaua     2100

uuuuuaagua cugcagugag ugcagacgua gguuguucag uugacuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9227
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 3 opt

<400>  9227
gggagaaagc uuaccaugag aauuauaaau gcaggcaaag aaaaaaaaag acgaggugca       60

gauacuaacg ugggaauagu aggauuauua cuaacaacag cgauggcagc cgaaguaacu      120

agacgaggaa gcgcauauua uauguaucua gaucgaaaug augcgggaga agcaauaagu      180

uucccaacaa cauuaggaau gaacaaaugc uauauacaaa ucauggaucu uggacacaug      240

ugugaugcaa caaugagcua ugaaugucca auguuagaug aaggaguuga accugaugac      300

guggauuguu ggugcaacac gacaucaaca uggguaguau auggaacaug ccaucacaaa      360

aaaggcgaag ccagaagaag cagaagagca guaacguuac cuagucauag cacaaggaaa      420

uuacaaacaa gaagucaaac uuggcuugaa ucaagagaau acacaaagca ucucaucaga      480

guugaaaacu ggaucuuucg aaauccagga uuugcuuuag cagccgcagc cauagcaugg      540

uuacuaggau caucaacaag ucaaaaagua auauacuugg ucaugauacu acuaauugca      600

ccagcuuaua guauacguug cauaggagua aguaacaggg auuuuguaga aggaaugucu      660

ggaggaacau ggguugaugu aguccuagaa cacggaggau guguuacggu aauggcacaa      720

gauaaaccaa caguagauau agaauuagua accaccacag uuucaaauau ggcagaagua      780

cgaucguauu guuacgaagc uaguauaagu gacauggcau cagauucaag augcccaaca      840

caaggagaag ccuauuuaga uaaacaaagu gacacacaau acguauguaa aagaacacua      900

guagauaggg gauggggaaa ugguugcggu uuauuuggga aggguaguuu aguaacgugu      960

gcaaaauuug cauguaguaa aaagaugacu ggcaaaagca uccaaccuga aaauuuagaa     1020

uauagaauaa uguuaucagu acauggaagu caacacucag gaaugaucgu aaacgauacu     1080

ggacacgaaa cugaugaaaa ucgagcaaaa guagaaauua caccaaacuc uccacgagca     1140

gaagcaacau uaggaggauu uggaucauua ggccuagauu gcgaaccccg aacaggacua     1200

gauuucagcg auuuauauua uuuaacaaug aauaauaaac auugguuagu acauaaagag     1260

ugguuucaug acauaccucu accguggcau gcaggagcag auacugguac accacauugg     1320

aauaauaaag aagcacuggu cgaauucaaa gaugcacaug caaaacgaca aacaguagua     1380

guauuaggca gucaagaagg agcgguacau acagcacuag caggagcccu agaagcagaa     1440

auggacggug caaaaggaag auuaaguagu ggacaucuga aauguagauu aaaaauggau     1500

aaacuaagau uaaaaggagu cucauauuca uuauguacag cagcauuuac auuuacaaaa     1560

auacccgccg aaacauuaca uggaacagua acaguggaag uacaauaugc uggaacagau     1620

ggaccuugua aaguuccagc ucaaauggca guagacaugc aaacauuaac acccguaggu     1680

agacuaauua cagcaaaccc uguaauaaca gaaagcacag aaaacucaaa aaugaugcua     1740

gaauuagauc ccccauuugg ugauaguuau auuguuauag gaguuggaga aaaaaaaaua     1800

acacaucauu ggcacagauc cgguucgaca auuggaaaag cauuugaagc uacuguaaga     1860

ggugcaaggc ggauggcggu auugggagac acugcuuggg acuucggauc aguuggagga     1920

gcacuaaacu cacuaggaaa agguauacau caaauuuuug gugcagccuu uaaaucauua     1980

uucggaggca uguccugguu cucacaaaua cuaauaggua cacuucuaau gugguuagga     2040

cuaaauacga aaaauggauc aauaagcuua auguguuuag cccuaggggg aguccuaaua     2100

uucuuaucaa cugcaguauc agcagaugua gguuguagug uagauuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9228
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 3 opt

<400>  9228
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaaaaaacg ccgaggagca       60

gaaacuagug uagggauagu agguuuacuu uuaacaacag caauggcagc agaaguuaca      120

agaagaggaa gugcuuauua uauguauuua gaccgcaaag augcaggaga agcaauaagu      180

uuucccacaa cguuggguau gaauaaaugu uacauacaaa uuauggacuu aggacacaug      240

ugcgaugcaa cuaugagcua cgaaugcccu augcuagaug aaggggucga accagaugac      300

guugauuguu gguguaacac aacaucaaca uggguagucu augguacaug ccaucauaaa      360

aaaggugaag cucgacgauc aagaagagca guaacacuac cuagucauag uacuagaaaa      420

cuacaaacaa gaagucaaac auggcuagaa aguagagagu auacaaaaca ccuaauaaga      480

gucgaaaauu ggauuuuuag aaacccagga uuugcauugg cggcagcugc uauugcaugg      540

uuacuaggaa guucaacuuc acaaaaaguu auauaucuag uaaugauacu auuaauagca      600

ccugcguauu caauuagaug uauaggaguu ucgaauagag auuuuguaga agggauguca      660

gguggaacau ggguagacgu aguacuagaa cacggaggau guguaacagu cauggcacaa      720

gauaaaccaa caguugacau agaauuagua acaacaacag uuaguaauau ggcagaaguc      780

agaucauauu gcuaugaagc cagcauaucu gauauggcaa gcgacucaag auguccaaca      840

caaggugaag cguaucuuga uaaacaauca gauacccaau auguauguaa gcgaacauua      900

guagauagag gguggggaaa uggaugcgga cuguuuggaa aaggaagucu agugacaugc      960

gcaaaauucg cauguucaaa aaaaaugaca ggaaaaagca uacaaccgga aaauuuagaa     1020

uauagaauaa uguuaagugu acaugguuca caacauagug gaaugauagu caaugauacu     1080

ggacacgaaa cagaugaaaa ucgagcaaaa guugaaauaa ccccuaauuc accaagagca     1140

gaagcuacau uaggagguuu uggaagucua ggauuagacu gugaaccaag aacagggcua     1200

gacuuuagcg auuuauauua uuuaacaaug aauaauaaac auugguuggu acauaaagaa     1260

ugguuucacg acaucccacu accguggcau gcuggagccg acacugguac accacauugg     1320

aauaauaagg aagcauuagu agaauuuaaa gaugcacacg caaaaaggca aacaguugua     1380

gucuugggca gucaggaggg agcaguucau accgcucuag cuggagcauu agaagcagaa     1440

auggauggag caaaagguag auuaucaucu ggacaccuaa aauguagacu uaaaauggac     1500

aaauugagac uuaaaggugu cucauauucc uuauguacag cugccuuuac auuuacaaaa     1560

aucccagcug aaacauuaca uggaacagug acaguggaag uacaauacgc aggaacagau     1620

ggaccuugua aaguuccagc acaaauggca guugauaugc aaacacuaac accaguuggc     1680

agauuaauua cggccaaccc agugauaaca gaaaguacag aaaauucaaa gaugauguua     1740

gaacuagauc cuccuuuugg agauucauau auugucauag gaguaggaga aaaaaaaaua     1800

acucaucauu ggcauagauc aggaucaaca auaggaaaag cauucgaagc aacaguuaga     1860

ggagcuaaaa ggauggccgu acuaggagac acagcauggg auuuuggauc agugggagga     1920

gcauuaaacu cauugggaaa agggauacac caaauuuuug gcgcagcauu uaaaucuuua     1980

uuugggggca ugucuugguu cagucaaaua uuaauuggaa cacugcucau guggcuugga     2040

cuuaauacaa aaaacggauc aauaagcuua augugucuug cauuaggagg aguucuaaua     2100

uuuuuaagua cugcaguauc ugcagaugua ggauguagug uggauuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9229
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 3 opt

<400>  9229
gggagaaagc uuaccaugag aauaauaaac gcaagaaagg aaaaaaaaag aagaggagca       60

gauacaagug uaggaauagu aggcuuacua cuuaccacag caauggcugc cgaaguaaca      120

agaagaggau cagcauauua cauguauuua gauagaaacg augcaggaga agcaaucucu      180

uuuccaacca cacucgggau gaacaaaugu uacauacaaa uaauggacuu aggacauaug      240

ugugaugcaa cuaugaguua ugaauguccu augcuagaug agggggucga accugaugau      300

guugauuguu gguguaauac cacuagcaca uggguagugu augguacaug ucaucacaaa      360

aaaggugaag caagaagauc aagaagagca guaacauuac caagucauag caccagaaaa      420

uuacaaacaa gaucgcaaac augguuagaa aguagagaau auacuaaaca ucuaauaagg      480

guagaaaacu ggauauuuag aaauccaggc uuugcauuag cagcggcugc aaucgcaugg      540

uuacuaggua guucaacauc acaaaaaguu auauaucuag uuaugauacu auuaauagca      600

ccagcauacu caauaagaug cauaggagua aguaauagag auuucguaga agguaugagu      660

gggggcacau ggguugacgu uguuuuagaa caugguggau guguuacugu aauggcacaa      720

gacaaaccaa cuguagauau agaacuagua acgacaacag uaucaaacau ggcugaagug      780

agaucauauu guuaugaagc caguauaagu gauaugguca gugauucaag auguccaaca      840

caaggugaag cauauuuaga uaaacaauca gacacucaau acguuuguaa aaggacucuc      900

guagacagag gauggggaaa cggaugugga uuauuuggua aagggucuuu aguaacaugc      960

gcaaaauuug cguguagcaa gaaaaugacc ggaaaaagua uucaaccuga aaauuuagag     1020

uauagaauua ugcuaagugu acacggaagu caacauucag guaugauagu uaacgauaca     1080

ggucaugaaa cagaugaaaa uagagcuaaa guagaaauaa caccaaauuc acccagggca     1140

gaagcaacau uagggggcuu cggaucauua ggacuagauu gugaaccucg aacugggcuc     1200

gauuuuagug aucuuuauua uuugacaaug aauaauaaac acuggcuugu gcauaaagaa     1260

ugguuucacg auauaccauu accauggcac gccggagcgg auacaggaac uccacauugg     1320

aacaacaaag aagcauuggu agaauuuaaa gacgcccaug cuaaaagaca aacagucgua     1380

guacuaggau cucaagaagg ugcaguacau acagcgcuag caggagcguu agaagcggaa     1440

auggauggug cuaaaggaag auuaagcagc ggacaucuga aaugcagauu aaaaauggac     1500

aaauuaagau uaaaaggagu cucauauuca uuauguacag cugcauuuac cuucacaaaa     1560

auaccagcag agacuuugca ugguacagua acaguagaag uacaauaugc uggaacagac     1620

ggucccugua aaguaccugc acaaauggcg guagauaugc aaacacuaac ucccguagga     1680

agacuaauaa cagcuaaucc gguuauaacc gaaaguacug aaaauagcaa aaugauguua     1740

gaauuggacc caccauuugg agauaguuau auuguuauag guguaggcga aaaaaaaaua     1800

acucaccauu ggcauagaag ugguaguaca auaggcaaag cauucgaagc aacuguaaga     1860

ggagcaaaaa gaauggcugu acuaggcgau acagcauggg auuucgguag cguaggagga     1920

gcacuaaacu cuuuagguaa agguauucac caaauauuug gugcagcauu uaagucccua     1980

uucggaggaa ugagcugguu uagucaaauc uuaauaggaa cucuacuaau gugguuagga     2040

cuaaauacaa aaaaugguag uauaaguuua augugcuuag cacuuggagg aguauuaauu     2100

uuucuaagca cagcaguauc agcagacguu ggaugcucug uagauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9230
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 3 opt

<400>  9230
gggagaaagc uuaccaugag aauuauaaau gcccgaaaag aaaagaaaag aagaggagca       60

gauacaaaug uagguauagu cggccuacua cuaacaacag caauggcagc agaaguuaca      120

cgccguggau cagcuuauua uauguaccua gauagaaaug augcaggaga agcgauuagu      180

uuuccgacaa cauuagguau gaauaaaugu uacauacaaa uaauggaccu gggacacaug      240

ugcgacgcua caaugagcua ugaaugccca augcuagacg aaggaguaga accagaugac      300

guagacugcu gguguaauau gacaucaaca ugggucguau auggaacaug ccaucauaag      360

aaaggagaag caagaagauc ucggagagcg guaacucucc caagucauag uacuagaaaa      420

cuacaaacaa gaucucaaac cuggcuagaa ucaagagaau auacuaaaca ucuaauaaga      480

guagaaaauu ggauauuuag aaacccuggu uucgcccuag cagcugcugc aauagccugg      540

cuacuaggaa guaguacaag ccaaaaagua auauauuuag uaaugauauu acucauugcu      600

ccggcguaua guauuagaug uauuggagua aguaauagag acuucguaga aggaaugucg      660

ggaggcacau ggguagaugu aguacuagaa cauggaggau guguaacggu uauggcucaa      720

gauaaaccaa caguagauau agaauuaguc acuacaacag uaucaaauau ggcagaagua      780

agaucuuacu guuaugaagc aucaauaagu gauauggcua gcgacucuag augcccaacc      840

caaggagaag cauacuuaga uaaacaauca gauacacaau auguauguaa aagaacauua      900

guagacagag gguggggaaa uggaugugga uuauucggaa aaggaucccu aguaacaugc      960

gcaaaauuug ccuguucaaa gaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa     1020

uauagaauaa uguuaagugu acacgguuca caacauucag gaaugauagu aaaugauacc     1080

ggucaugaaa cugaugaaaa uagagcaaaa guggaaauca ccccaaauuc accaagggca     1140

gaagccacuc uaggaggguu ugguucacua ggacuugauu gugaaccaag aacaggacua     1200

gauuuuucag auuuauauua uuuaaccaug aauaauaaac auugguuagu ucauaaagaa     1260

ugguuucaug auauuccccu uccauggcau gcaggagcag acacaggaac accucauugg     1320

aacaacaaag aagcucuagu agaauuuaaa gaugcacacg caaaaagaca aacuguaguc     1380

guucuaggau cacaagaagg agcaguacac acagcacuag caggagcacu cgaagcagaa     1440

auggauggag caaaaggacg acuaagcagu ggacauuuaa aaugucgacu uaaaauggau     1500

aaauuaagau uaaaaggagu aaguuacuca uuauguacag cagcauuuac auucacaaaa     1560

auacccgcag aaacccuaca uggaacagua acuguggaag uccaauacgc uggcacagau     1620

ggaccaugua aaguaccagc ucaaauggca guagacaugc aaacauuaac uccaguaggu     1680

agauuaauaa cugcaaaucc ugucauaaca gaaucaacgg aaaacuccaa aaugauguua     1740

gaacuagacc caccauuugg agacaguuau auaguuauag guguagguga aaagaaaauu     1800

acacaucauu ggcauagaag uggaucaacc auaggaaaag ccuuugaagc aacaguaaga     1860

ggagccaaaa ggauggcagu acuuggagac acagcauggg acuuuggaag cguaggagga     1920

gcauuaaaca gccugggaaa aggaauacau caaauauucg gagcagcauu uaaaagccuu     1980

uuuggaggua ugucgugguu cucccaaauc cuaauuggaa cucucuuaau guggcuagga     2040

cuuaauacca aaaacggauc aauaucauua augugucuag cauuaggagg uguacuaaua     2100

uuucuaagua cagcaguauc agcagacgua ggauguucag uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9231
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 3 opt

<400>  9231
gggagaaagc uuaccaugag aauaauaaac gcuaggaaag aaaaaaaaag aagaggggca       60

gacacaagug uagggauagu cggacuauua uuaacuacag caauggcagc agaaguuaca      120

aggagagguu cugcauacua uauguauuua gaucgaaaug acgcaggcga ggcuauaagu      180

uuucccacaa cucucggaau gaauaaaugu uauauucaaa uaauggaucu uggacacaug      240

ugugaugcaa ccaugucaua ugaaugucca auguuagaug aaggaguaga gccagacgau      300

guagacuguu gguguaacac uacaucaacu uggguaguuu acggaacaug ucaucauaaa      360

aaaggagaag cgagaaggag uagaagagca guaacauuac cuucacauuc aaccaggaaa      420

uuacaaacaa gaagccaaac cugguuagaa ucaagagaau auacaaagca uuuaauacgc      480

guggaaaacu ggauauuuag gaacccaggc uucgcuuuag cagcggcugc gauagcaugg      540

cuccucggau caucaacguc acaaaaagua auauauuuag uaaugauuuu auugauagca      600

ccagcauauu caauacgaug uauuggcgug ucaaaucgag auuuuguuga aggaaugucc      660

ggaggaacuu ggguagaugu aguauuagaa cauggaggau guguaacagu aauggcacaa      720

gacaaaccua caguugauau agaauuagua acaacaaccg uaucaaauau ggcagaggua      780

agaagcuauu guuaugaagc uucaauauca gauauggcuu cagauagcag auguccaaca      840

cagggagaag cuuaucuaga caaacaauca gacacacaau auguauguaa aaggacacua      900

guagauagag guuggggaaa ugguuguggu cuuuucggua aaggaagcuu aguaaccugc      960

gcaaaauuug cuuguaguaa aaaaaugacu ggaaaaagca uucaaccaga aaauuuagaa     1020

uauagaauaa uguuaagugu acacggcucu cagcauagcg gaaugauagu aaaugauacc     1080

ggccacgaaa cugacgaaaa uagagccaaa gucgaaauua cuccuaauuc uccaagggcu     1140

gaagcugcau uaggugguuu uggaucgcua ggccuugacu gcgaaccaag aacaggauua     1200

gacuucagug aucuauauua uuuaaccaug aauaauaaac acugguuagu ccauaaagaa     1260

ugguuccaug auauaccgcu accauggcac gcaggugcag auacagguac cccacacugg     1320

aauaauaagg aagcauuagu cgaauuuaaa gaugcacacg cgaaaagaca aacaguagua     1380

guauuaggau cacaagaagg ggcuguacac acagcacuag cuggagcacu agaagcagaa     1440

auggauggug ccaaaggcag auuaaguagu ggacaucuua aauguagacu uaaaauggau     1500

aaauuacgac uuaaaggugu aaguuauuca cuauguacag cagcauuuac uuuuacuaaa     1560

auuccagcag aaacacuuca uggcaccgua acuguugaag uacaauacgc aggaacugau     1620

gguccuugua aaguuccagc acaaauggca guggauaugc aaacauuaac cccaguaggc     1680

agauugauaa cugcaaaucc gguaauuaca gaaaguacag aaaacaguaa aaugaugcuu     1740

gagcuugauc caccauuugg ggacucuuac auaguaauag gagucgguga aaaaaaaaua     1800

acacaccacu ggcacagauc agguucaacu auaggaaaag ccuucgaagc uacaguaaga     1860

ggugcaaaaa gaauggcagu auuaggagau acagcauggg acuucggauc uguaggagga     1920

gcauuaaauu cauuaggaaa aggaauacau caaaucuucg gagccgcauu uaaaagucua     1980

uucgguggaa ugucuugguu uagccaaaca uuaauaggca cuuuauuaau guggcuaggu     2040

uuaaauacaa aaaaugguuc aauuaguuua auguguuuag cauuaggagg uguacuaaua     2100

uucuugagua ccgcaguauc agccgauguc gguuguucug uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9232
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 3 opt

<400>  9232
gggagaaagc uuaccaugag gauuauaaac gcacgaaaag aaaaaaaaag aagaggggca       60

gaaacaagcg uaggaauugu aggucuacua uuaacaacag cuauggcugc ugaaguaaca      120

cgaagaggau cagcauauua cauguauuua gauagaaaug augcgggaga agcaaucuca      180

uucccuacaa cguuagggau gaauaaaugu uauauccaaa uaauggaucu aggccacaug      240

ugcgaugcua caauguccua ugagugccca augcuagaug aaggaguaga accagaugau      300

guagauuguu gguguaauac aacaaguaca uggguaguau augguacaug ucaccacaaa      360

aaaggagaag cgaggcgguc aaggagagca guuacauuac caucacacag uacaagaaaa      420

cuacagacac gaucacaaac augguuggaa aguagggaau acacuaaaca ucuaauacgu      480

guugaaaauu ggaucuuuag aaacccaggg uucgcucuag cagcggcagc uauagcaugg      540

uuacuagguu cuucaacauc gcaaaaagua auauaccucg uaaugauucu acuaauagcc      600

uuagcuuacu caauaagaug cauaggcgua uccaauagag auuuuguaga aggaauguca      660

ggaggaacuu ggguagaugu aguuuuagaa cacggaggcu guguaacagu aauggcacag      720

gacaaaccua caguagauau agaauuagua acaacaacag uaucuaauau ggcagaaguu      780

cgaucauauu guuaugaagc aucgauuuca gauauggcaa gcgauaguag auguccuacg      840

caaggugaag cauauuuaga uaaacaaucu gauacucaau auguaugcaa aagaacauua      900

guagauaggg guuggggaaa uggaugugga cuauucggaa aaggaagcuu ggucacuugu      960

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa     1020

uacagaauaa ugcugagcgu acauggaagc caacauagcg gaaugauagu aaacgacaca     1080

ggacaugaaa cagaugaaaa cagagcaaaa guagaaauaa cucccaauuc accaagagca     1140

gaagcaacuc ucggaggauu cggaagcuua ggacuagacu gugaaccgag aacaggucua     1200

gacuucucug aucucuacua ucuaacuaug aauaacaaac auuggcuagu ucauaaagaa     1260

ugguuccaug acauaccauu accauggcau gcuggagccg auacaggaac accacauugg     1320

aacaauaaag aagcucuagu ugaauuuaaa gaugcacaug caaaaagaca aaccgucguu     1380

guauuaggau cacaagaggg agcuguccau acagcauuag caggagcauu agaagccgaa     1440

auggauggag caaaaggaag acuaucauca ggacauuuga aauguagauu aaaaauggau     1500

aaauuaagac uaaaaggggu aucauacuca cuauguaccg cagcauuuac auuuaccaaa     1560

auaccagcag aaacauuaca uggaacaguu acaguggaag uccaauaugc cggaacugau     1620

gguccaugca agguacccgc ccaaauggca guagacaugc aaacacuaac accaguggga     1680

agauuaauaa ccgccaaucc aguaauuaca gaaucaacag aaaauucaaa aaugaugcuc     1740

gaauuagauc cgccauucgg agauagcuac auuguuauug gaguaggaga aaaaaaaauu     1800

acacaccauu ggcaccgaag cggaagcacu auuggaaaag cauuugaagc uacuguaaga     1860

ggagcaaaac gaauggcagu acuuggagau acugcuuggg auuuuggaag uguagguggc     1920

gcacuaaaca gcuuagguaa aggaauacau caaauauucg gcgccgcauu caaaagcuug     1980

uuugggggaa ugucaugguu cucacaaaua uugauaggua cacuuuugau guggcuagga     2040

cuaaauacaa aaaacgguuc aauaagucuu augugucuag cccucggugg aguacuuaua     2100

uuuuuaagua ccgcaguaag cgcagauguu ggcuguagug uagacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9233
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 3 opt

<400>  9233
gggagaaagc uuaccaugag aauaauaaau gcgagaaaag aaaaaaaaag aagaggagcu       60

gauacaagug uuggaauugu gggucucuua uuaacaacag caauggcagc agaaguaaca      120

agaagagggu cagcauacua uauguaucua gauaggaaug augccggcga agcuauauca      180

uuuccuacaa cauuaggaau gaauaaaugc uauauacaaa uaauggaucu uggucauaug      240

ugugaugcaa ccaugaguua ugaauguccg auguuggacg aaggaguuga accagaugau      300

guagacugcu gguguaauac aacaaguaca ugggucguau auggcacaug ucaucauaaa      360

aaaggagaag cgagaagauc gcguagagcu guaacacuac caucacauag uaccagaaaa      420

cuacaaacga gaagucaaac auggcuagaa ucaagagaau auacaaaaca cuuaauacga      480

guagaaaauu ggauauuuag aaacccagga uucgcauuag cagcagcggc aauugcaugg      540

uuauuaggau caaguacgag ucaaaaagua auauauuuag uaaugauacu auuaauagca      600

ccugcauacu caauaagaug uauaggaaua aguaaucgag auuucgugga aggaauguca      660

ggagguacau ggguagaugu aguucuugaa cauggaggau guguaacugu aauggcccaa      720

gacaagccaa caguagauau cgaauuggua acaacgacag uuaguaauau ggcagaagua      780

cgaaguuauu guuacgaagc aucuauuucu gauauggcau cagacaguag auguccaaca      840

caaggagagg cuuaucugga caaacaaagu gacacacaau auguauguaa acguacgcua      900

guugaucgag gauggggaaa cggauguggg uuauucggaa aagggaguuu aguaacaugu      960

gcaaaauuug cauguucgaa aaagaugacg ggaaaaagca uacaaccaga gaauuuggaa     1020

uauagaauaa ugcuaagugu gcacggauca caacacucag guaugauagu caaugauacu     1080

ggacaugaaa ccgaugaaaa ucgugcaaaa guagaaauua caccaaauag uccaagagca     1140

gaagcaacac uaggcggauu cgggauauua ggauuagauu gugaaccaag aacaggacua     1200

gauuuuagcg aucuauacua cuuaaccaug aauaacaaac auugguuagu acauaaagag     1260

ugguuccaug auaucccucu uccauggcac gcuggugcag auacaggaac accacauugg     1320

aacaacaaag aagcacuagu agaauuuaaa gaugcacacg caaaaagaca aacaguagua     1380

guauuaggaa gccaagaggg agcaguacau acagcacuag caggugcauu agaagcagaa     1440

auggacggag cgaaagguag auugaguagu ggacauuuaa aaugcagauu aaaaauggac     1500

aaacuaagac uuaaaggagu aaguuacagu cuuuguacag cagcauuuac uuucacaaaa     1560

auaccagcug aaacacuuca uggaacaguu acuguagaag uucaauaugc agguacagau     1620

ggaccaugua aaguacccgc acaaauggca guagauaugc aaacccugac accaguaggc     1680

agacuaauaa cagcgaaccc aguaauaaca gaaagcaccg aaaacucaaa aaugaugcua     1740

gaauuagacc caccauuugg agacaguuau auaguuauag guguuggaga aaaaaaaaua     1800

acacaucacu ggcacagaag ugguaguaca auaggcaaag cauuugaagc uacugucaga     1860

ggcgcaaaaa gaauggccgu auuaggagau acagcauggg acuuugggag cguuggaggu     1920

gcauugaaua gccucgguaa agggauccau cagauauuug gagcagcauu uaaaagcuua     1980

uucggaggaa ugucaugguu cucacaaaua uuaauuggua cauuauuaau gugguuaggu     2040

uugaacacaa agaaugguuc aauuucauua augugcuuag ccuuaggagg aguauuaaua     2100

uuucuuagua cagcaguauc ggcugauguu ggauguuccg uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9234
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 3 opt

<400>  9234
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaagaaaag aagaggagca       60

gauacaagug uaggaauagu gggucuauua uuaacgacug caauggcagc cgaagugaca      120

agacgaggaa gcgcauauua uauguaucua gauaggaaug augcuggaga agcaauuuca      180

uuuccaacca cuuuaggaau gaauaaaugc uauauucaaa uaauggaucu aggacauaca      240

ugugaugcaa caaugaguua ugaaugucca auguuagaug aaggcguaga accagaugac      300

guagacuguu gguguaauac uacaaguacu uggguagucu auggaacaug ccaucauaag      360

aaaggagaag cucgacgguc acguagagcc guaaccuuac cuucucacuc aauaagaaaa      420

uuacaaacac gaagucaaac auggcuagaa agcagagaau auacaaaaca ucuaauacga      480

gucgaaaauu ggauauuucg aaauccggga uuugccuuag ccgcggcagc aauagcgugg      540

uuacucggua guucaacauc acaaaaagua auuuacuugg uaaugauacu gcuuauagcc      600

ccagccuacu caauaagaug uauaggagua aguaauagag auuuuguuga aggaauguca      660

ggcggaacuu gggucgaugu aguauuagaa cacggcgguu gugugacagu uauggcccaa      720

gacaaaccaa caguagauau agaauuagua acaacuacag uauccaauau ggcagaagua      780

agaaguuauu guuaugaagc aucaauaucg gauauggcau ccgauaguag augucccaca      840

caaggagaag cauaucuaga uaaacaauca gacacacaau auguauguaa acgcaccuua      900

guagacagag guuggggaaa cggaugugga uuauuuggaa aaggaagucu cgugacuugc      960

gcuaaauuug cauguagcaa gaaaaugaca ggcaaaagua uacagccaga aaauuuagaa     1020

uauagaauaa uguuaagugu acauggaagu caacauagug gaaugauugu aaaugauaca     1080

ggacaugaaa cagaugaaaa cagagcaaaa gucgaaauaa caccaaacuc accacgagca     1140

gaagccacac uaggaggauu uggaagccua ggcuuagauu gugaaccaag aacaggacua     1200

gacuuuucag auuuguacua uuuaacuaug aauaauaaac acuggcuagu ucauaaagaa     1260

ugguuccaug acauaccauu accauggcac gcuggugcag auacagggac accacacugg     1320

aauaauaaag aggcuuuggu agaauuuaaa gaugcucacg ccaaaagaca aacaguagua     1380

guauuaggau cacaagaagg agcuguacau acagcauuag cuggugcguu agaagcagaa     1440

auggauggag caaaaggucg auuaagcuca ggacacuuaa aauguagauu aaaaauggac     1500

aaauuaagac uaaaaggugu aucauauagu cuaugcacag cugcauucac auucacaaaa     1560

auacccgccg aaacacuuca ugguacaguc acagucgaag uccaauaugc aggaacugau     1620

ggaccaugca aaguaccugc gcaaauggcu guagacaugc aaacgcuaac uccaguagga     1680

agauuaauaa cagcaaaucc agucauuacu gaaucaacug aaaauaguaa aaugaugcua     1740

gaauuagauc caccauuugg ugauuccuau auaguaauag gcguagguga aaagaaaaua     1800

acucaucauu ggcacagaag ugggucaacu auaggaaagg cuuucgaagc cacaguaaga     1860

ggugcaaaaa gaauggcagu auuaggagac acagcauggg acuuuggaag uguaggagga     1920

gcauuaaaca gucuagguaa aggaauacau caaauauuug gagcagcauu caaaucauua     1980

uucggcggaa ugagcugguu uucacaaaua uuaauaggaa cccucuuaau gugguuagga     2040

cuaaaugcua aaaauggauc aauuucacuu augugucucg cacuaggagg aguccuaaua     2100

uuuuugucga cagcaguauc agcagaugua gguuguucug uggacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9235
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 3 opt

<400>  9235
gggagaaagc uuaccaugag aaucaucaau gcuagaaaag aaaaaaaaag acgcggggca       60

gauacaagug uuggaauugu aggauuguua cuaacaacag caauggcagc agaaguaaca      120

agacgaggga gugcauauua cauguaucua gauaggaaug augcagggga agcuauaagc      180

uuuccaacaa cucuagguau gaauaaaugc uauauacaaa uaauggaccu gggucauaug      240

ugugaugcaa caaugagcua ugaaugucca auguuagaug aaggaguaga accagaugac      300

guagacuguu ggugcaauac aacaaguaca uggguaguau auggaacaug ucaucauaaa      360

aaaggagaag cacaacgaag cagaagagcu guaacacuac caagccacuc uacuagaaaa      420

uuacaaacca gaucacaaac guggcuggaa ucacgugaau auacaaaaca ucuuauuaga      480

guagaaaauu ggauauucag aaauccgggg uuugcauuag cagcagcugc aauagcaugg      540

uuacuaggua guucuacuuc ccaaaaaguc auauacuuag uuaugauauu auuaauagca      600

ccagcuuaca gcauacgaug uauaggcguu aguaauaggg auuuuguaga agguauguca      660

ggaggcacau ggguagaugu aguuuuagaa cacggcggau gugucacugu aauggcgcaa      720

gacaagccaa caguagacau agagcuuguu acaacaacgg uaucaaauau ggcagaagug      780

aggucauauu guuacgaggc aucaauaagu gauauggcau cagauucaag auguccaaca      840

caaggagagg ccuauuuaga uaaacaaagc gauacacaau acguaugcaa aagaacacug      900

guugauagag gauggggaaa uggaugugga cuguuuggaa aaggaaguuu agugacaugc      960

gcgaaauuug ccuguuccaa aaaaaugaca ggaaaaagca uacaaccaga gaauuuagaa     1020

uauagaauaa uguuaagugu gcacggaagu caacacagug gaaugauugu aaaugacaca     1080

ggucacgaaa cagaugaaaa uagagcuaaa guggaaauaa cgccuaauuc accaagggca     1140

gaagcaacau uaggaggauu cggaaguuua gggcuagacu gugaaccaag gacaggguua     1200

gauuuuagcg aucuguauua ucugacgaug aacaacaaac auugguuagu acauaaagaa     1260

ugguuccacg auauaccauu accguggcau gccggagcag acaccggcac accacauugg     1320

aauaauaaag aagcauuagu agaauuuaaa gacgcacaug caaaacguca aacaguagua     1380

guucuaggaa gucaagaagg ugcaguacac acugcauuag ccggagcacu agaggcggaa     1440

auggacggag caaaaggucg acuuucaagu ggucaucuaa aauguagauu aaaaauggac     1500

aaacuucgac uaaaaggagu aucuuauagu uuaugcacag cagcauucac auuuacgaaa     1560

auuccagcag aaacauuaca uggaacagug acuguagagg uacaguacgc gggaacagau     1620

ggaccaugua aagugccagc acaaauggca guugacaugc aaacauugac accaguagga     1680

cgacucauca cagcaaaucc cguaauaacc gaaucaacgg aaaauucaaa aaugauguug     1740

gaauuagacc caccauuugg ggacaguuau auuguaauag gaguuggaga aaaaaaaauu     1800

acucaucacu ggcacaggag cggaucaaca auagguaaag cguucgaagc aacuguucga     1860

ggagccaaac gaauggcagu auuaggagau acagcauggg auuuugguuc aguaggagga     1920

gcauuaaaca guuuaggaaa aggaauacac caaauauuug gagcggcauu uaagucauug     1980

uuuggaggga ugucaugguu cucacaaaua cuaauaggaa cacuguuaau gugguuagga     2040

uuaaauacca aaaacggcag uauuaguuua augugcuuag cacuaggagg aguguuaaua     2100

uucuuaagua cugcuguaag ugccgaugua ggcuguagug ucgacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9236
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 3 opt

<400>  9236
gggagaaagc uuaccaugag aauaauaaac gcaagaaaag aaaaaaagag aagaggagca       60

gauaccagug uagguauugu aggucuguua uugacaacag cuauggcagc agaaguaaca      120

agaagaggaa gugcuuauua cauguauuua gauagaaaug augcaggaga agcaauauca      180

uucccaacaa cauuaggaau gaauaaaugc uauauacaga uaauggacuu aggacacaug      240

ugugaugcaa cuaugucgua ugaaugucca augcuagaug aaggaguaga accggaugau      300

guugauugcu gguguaauac aacgucaaca uggguaguuu auggaaccug ucaucauaag      360

aaaggggaag cuagacguuc aagaagagca guaacccuac caucacacag cacgcgaaaa      420

uuacaagcaa gaucacagac uugguuagaa ucacgagaau auacgaaaca cuuaauacga      480

guagagaauu ggauauuuag aaauccagga uuugcauuag cagcagccgc uauagcaugg      540

uuacuuggau caucaacauc ccaaaaagua aucuaccuag uaaugauacu auuaauagca      600

ccagcuuaua guauuagaug uauaggagua aguaauagag auuuuguuga aggaaugagu      660

gguggaacgu ggguagaugu aguauuagaa cauggcggau guguaaccgu uauggcacaa      720

gacaaaccaa caguggacau agaacuaguu acaacaacug uaagcaacau ggcggaagua      780

aggucauauu guuacgaagc uagcauaagu gacauggcau cagauaguag auguccaaca      840

caaggcgaag ccuaucuaga uaaacaauca gacacacaau auguauguaa gagaacacuu      900

guagaucgug gauggggaaa cgguugugga uuguuuggua aaggaucacu aguuacuugu      960

gcaaaauucg cauguaguaa aaaaaugacg ggaaaaagca uacaaccaga aaauuuagag     1020

uacagaauaa ugcugagugu ucaugguuca caacauucag gaaugauagu aaacgauaca     1080

ggucacgaaa cugaugaaaa cagagcgaaa guagaaauaa cuccaaauag uccuagagcc     1140

gaagcaacuu uaggcgguuu ugguucauua ggauuagacu gugaaccaag aacuggauua     1200

gauuucucug auuuauacua cuuaacuaug aauaauaaac auuggcuagu ccauaaagaa     1260

ugguuucaug acauaccauu acccuggcac gcaggugccg acacagguac accacauugg     1320

aauaauaaag aagcauuagu ugaauuuaaa gaugcacaug caaaaagaca aacaguagua     1380

guauuaggga gucaagaagg agcaguacau acagcuuuag caggggcauu ggaagcagaa     1440

auggauggcg cuaaagguag acuaucaucu ggacacuuaa aaugucgacu aaaaauggau     1500

aaauuaagac uuaaaggagu aaguuauagc cuaugcacgg cagcguuuac auuuacaaaa     1560

auaccugcug aaacuuuaca uggcacaguu acaguagaag uacaguaugc gggaacagau     1620

ggucccugua aaguaccagc ucaaauggcu guagauaugc aaacguuaac accaguaggc     1680

agauuaauaa cagcaaaccc aguuauaaca gaaaguaccg aaaauagcaa aaugauguug     1740

gaauuagauc caccuuuugg agauucauau auaguaauag gaguuggaga aaaaaaaaua     1800

acacaucauu ggcauagaag uggaucaacc auaggcaaag cuuuugaggc aacaguaagg     1860

ggagcaaaac gaauggcagu auuaggagau acagcauggg auuuugguuc aguaggagga     1920

gcacucaaua gucuuggcaa aggcauacau cagauauuug gagccgcuuu caaaucauua     1980

uuugguggga ugucaugguu cucacaaauu cugauaggaa cacuguuaau gugguuaggc     2040

cuuaauacga aaaauggauc aauuagcuua augugcuuag cauuaggagg aguacuaauu     2100

uuuuuaucaa ccgcaguaag cgcugaugua gggugcagug uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9237
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 3 opt

<400>  9237
gggagaaagc uuaccaugag aauaauaaau gcacgaaaag agaagaaaag acggggcgca       60

gacacaagug ucggaauugu aggauuacuu cuaacaacag caauggcagc agaaguaaca      120

agaagaggaa gugcauauua uauguaucug gauagaagcg augcaggaga agcaauuagc      180

uucccaacaa cguuagguau gaauaaaugc uacauacaaa uaauggauuu aggacauaug      240

ugcgacgcaa caaugaguua cgaaugucca augcuggaug aaggaguaga accagacgau      300

guggauuguu ggugcaacac uacaaguacu ugggugguau auggaacuug ccaucacaag      360

aaaggugaag caagaagauc aaggagagcu guuacguuac caucacacag uacaagaaaa      420

uuacaaacaa gaagucaaac auggcuggaa aguagagaau acacaaaaca cuuaauaaga      480

guugaaaauu ggaucuuuag gaacccagga uuugcacuag cugcagcagc aauagccugg      540

cuacuaggua guaguacaag ccaaaaagua auauauuuag ucaugauccu auuaauagca      600

ccagcauacu cuauaagaug uauaggagua agcaauagag auuucguaga aggaaugucu      660

ggaggaaccu ggguagaugu aguuuuagaa cacggaggcu gcguaacagu uauggcccaa      720

gauaaaccga caguugauau agaacuugua acaacaacgg uaaguaauau ggcagaagua      780

agauccuacu guuacgaagc aaguauaagu gacauggcuu cagauucaag auguccaaca      840

caaggggaag cguaccuuga uaaacaaucu gauacacaau auguuuguaa aagaacccua      900

guagauagag guuggggaaa uggaugugga cuuuuuggaa aagggaguuu aguuacaugu      960

gcaaaauucg cauguaguaa gaaaaugaca gguaaaagua uacaaccaga aaauuuggaa     1020

uacaggauaa uguuaagugu ccauggaagu caacauagcg gaaugauagu caaugauacg     1080

gggcaugaaa cagacgaaaa cagagcaaaa guagaaauaa caccuaauuc cccgcgagca     1140

gaagcaacau uagguggauu uggcucauua ggauuagauu gcgaaccaag gacuggauua     1200

gauuuuagug auuuguauua uuuaacaaug aacaauaaac auuggcuugu ucauaaagaa     1260

ugguuucaug auauuccacu accauggcac gcaggagcug auacaggcac cccacauugg     1320

aauaauaaag aagcauuggu cgaauuuaaa gaugcucaug caaaaagaca aacgguagua     1380

guauuaggau cacaagaagg agcaguacau acagcacuug cuggugcuuu agaagcagaa     1440

auggauggag caaaaggucg auuaaguagu ggacauuuaa aauguagauu aaaaauggac     1500

aaauugagac uaaaaggugu cuccuauagc uuaugcacag cagcauuuac auuuacaaaa     1560

aucccagcag aaacauuaca uggaacugua acuguugaag uacaauaugc agguacagau     1620

ggcccgugua aaguuccagc ucagauggcu guugauaugc aaacauuaac accugucgga     1680

agauuaauaa cggcaaaucc cguaaucacg gaaaguacug aaaauaguaa aaugaugcua     1740

gaauuagauc caccauuugg ggacagcuau auaguaauag gaguagguga aaagaaaauc     1800

acacaucauu ggcauaggag uggaagcaca auaggaaaag ccuuugaagc aacaguaaga     1860

ggagcaaaac gaauggccgu gcuaggugau acagcauggg auuucggauc agugggugga     1920

gcucucaauu ccuuaggaaa agggauacau caaauauuug gagcugcauu uaaaagucua     1980

uuugggggua ugucuugguu uagucaaaua uuaauuggaa cauuacuuau guggcuggga     2040

uuaaacacua aaaaugguuc gauaaguuua auguguuuag cauuaggugg gguuuuaauu     2100

uuucuaagua cagcaguauc cgccgaugug gguuguagug uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9238
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 3 opt

<400>  9238
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaagaaaag aagagguaca       60

gacaccagug uaggaauagu aggauuacua uugacaacag caauggcugc agaaguuaca      120

cgcagaggau cugcuuacua uauguaccua gauagaagug augcaggaga agccauuuca      180

uucccaacaa cuuuagguau gaauaaaugu uauauacaaa uaauggaucu aggacacaug      240

ugcgacgcaa cuaugaguua ugaaugcccu augcuagaug aaggaguaga acccgaugau      300

guugauuguu gguguaacac aacaaguaca uggguaguau acggaacaug ucaucauaag      360

aaaggcgaag caagaagaag cagacgagca guuacauuac cgagccauuc aacaagaaaa      420

cuacaaacaa gaucucaaac cugguuagaa agucgagaau auacaaaaca uuuaauuaga      480

guagaaaacu ggauauucag aaauccagga uuugcuuuag cagcugcagc uauagcaugg      540

cuacuaggau cauccacauc acaaaaaguu auauacuuag uaaugauucu auuaauagcc      600

ccagcauauu caauaagaug cauaggugua aguaauagag auuucgucga aggaaugagu      660

ggaggaacau ggguugacgu aguacuagaa cauggaggcu guguaacagu aauggcccaa      720

gauaaaccga cuguagauau agaauuagua acaacaacag uaaguaauau ggccgaaguc      780

agaucauacu guuaugaagc cagcauaagu gauauggcaa gcgauuccag auguccaacu      840

caaggugagg cauauuuaga uaaacaaucg gauacacaau augugugcaa aagaacucua      900

guagacagag gauggggaaa cggcugugga uuauuuggaa aaggaagucu aguaacaugu      960

gccaaauuug caugcucaaa gaaaaugacu ggaaaaucaa uacaaccaga aaaucuagaa     1020

uaucgaauaa uguuaagugu gcauggaagu caacacagcg gaaugauagu aaacgauacu     1080

ggucaugaga cagacgaaaa cagagcaaaa guagaaauaa caccuaauuc accacgagca     1140

gaagcaaccu uaggaggcuu cggaucccua ggauuagauu gugaaccacg aacaggcuua     1200

gacuuuagug auuuauauua uuuaacaaug aauaacaaac auuggcuggu acauaaagaa     1260

ugguuucacg auaucccauu accauggcac acaggugcag auacaggaac accacauugg     1320

aacaacaaag aggcgcuugu agaauuuaaa gaugcucacg caaaaagaca aacaguugua     1380

guauugggau cucaagaagg cgcaguacau acagcauuag cuggagcucu agaagcagaa     1440

auggacggug caaaaggccg acugucuucu ggucacuuaa aaugcagacu caaaauggau     1500

aaauuaagac uuaaaggcgu aucauacagu uuauguaccg cugcuuucac auuuacaaaa     1560

auaccagcug aaacuuuaca uggaaccgua acaguagaag ugcaauacgc ugguacugau     1620

ggaccaugua aagugcccgc ucaaauggca guagauaugc agacacuaac accaguggga     1680

cgauuaauua cugcaaaucc aguuauuaca gaaggaacag aaaacucaaa aaugauguua     1740

gaauuagauc caccauuugg ugauucauau auaguaauag gaguagguga gaaaaagauu     1800

acacaucauu ggcauagauc aggaucaaca auagggaaag cguucgaagc uacaguaaga     1860

ggagcuaaaa gaauggcagu auuaggagac acggcauggg auuuuggauc agugggugga     1920

guacucaauu cauugggaaa aggaauacac caaauauucg gagcagccuu caaaagccua     1980

uuuggaggaa ugaguugguu uagucaaaua cucauaggaa cauuguuaau guggcuaggc     2040

uuaaauacua aaaauggcag uauaucacua augugucuug cacugggagg aguacuuaua     2100

uuucuaucaa cagcuguauc cgcagaugua ggauguagcg uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9239
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 3 opt

<400>  9239
gggagaaagc uuaccaugag aauuauaaau gcaaggaaag aaaaaaaaag aaggggagcu       60

gauacaucag uaggaauagu uggucuucua cuaacuacag caauggcagc ggaaguaacg      120

cgaagaggau cagcauauua uauguaucua gauagaaaug augcaggaga ggcaaucucu      180

uuuccuacaa cauuaggaau gaacaaaugu uacauacaaa uaauggacuu gggacauaug      240

ugcgaugcaa ccaugucaua ugaauguccu auguuggaug aagguguaga accugaugac      300

guugauuguu ggugcaauac aacaucaaca uggguaguau acgggacaug ucaucacaaa      360

aaaggugaag cuagaagauc aagaagagcu guuacauuac cuucacacuc aacacgaaaa      420

uuacaaacaa gaucacaaac augguuagaa ucaagagaau auacaaaaca ccuaauaaga      480

guagaaaauu ggauauuuag aaauccaggu uuugcauuag cagcagcagc aauagcaugg      540

uuauuaggau ccucaacgag ucaaaaaguu auuuauuuag uaaugauuuu guuaauugcu      600

ccagcuuauu caauaagaug cauaggugua aguaaucgag acuuuguaga agggaugagu      660

ggugggacau ggguagaugu aguauuagaa cacggcggau gcguaacggc aauggcgcaa      720

gacaaaccaa caguagauau agaacuaguu acaacuacgg uuucuaacau ggcagaagua      780

agaucuuauu gcuacgaagc aucaauauca gacauggcau cugauaguag auguccaaca      840

caaggggaag cauaucuaga uaaacaauca gauacacaau auguauguaa acgaacucua      900

guagaccgug gcugggguaa cggaugcggg cucuuuggaa aaggaucauu aguaacaugu      960

gcaaaauuug cuuguuccaa gaaaaugaca ggaaaaagca uacaaccaga aaaccuugaa     1020

uauaggauaa uguuaucagu acauggcuca caacauagug guaugcuugu aaaugauaca     1080

ggacaugaaa cggacgaaaa ccgagcaaaa guugaaauaa cuccaaauag uccgcgagcu     1140

gaagcaacgu uaggcggauu uggaagcuua gggcuagauu gugaaccaag aacaggauug     1200

gauuucucag aucuuuauua uuuaacaaug aauaauaaac auugguuagc acacaaggaa     1260

ugguuccaug auauaccccu accauggcau gcaggugcag ccacgggaac uccucauugg     1320

aacaauaaag aagcauuagu agaauuuaaa gacgcacacg ccaaaagaca aaccgucguu     1380

guacuuggau cacaagaggg agcaguacau acagcauuag caggagcauu ggaagcagaa     1440

auggauggug cuaaaggaag auuaucuagc ggacaucuaa aaugcaggcu aaaaauggac     1500

aaacuacgau uaaaaggugu aagcuauagu cuaugcacag cagcuuuuac auuuacaaaa     1560

auacccgcag agacuguuga cgguacagua acaguagaag gucaauaugg agggacugau     1620

gguccuugca aaguuccagc acaaauggca gucgacaugc aaacacuaac gccaguuggu     1680

agacuaauaa cagcgaaucc cgucauuaca gaaucaacag aaaauucuaa aaugauguua     1740

gaauuagauc caccauuugg cgauagcuau auaguuaucg gaguaggcga aaaaaaaaua     1800

acucaucauu ggcaccggag uggaagcacu auaggaaaag cauucgaagc cacaguaagg     1860

ggugcaaaaa gaauggcagu gcuaggagau acugcauggg auuuuggaag uguagggggc     1920

gcauuaaaua guuuaggaaa aggaauacau caaauaauag gugcagccuu uaaaagucua     1980

uucggaggaa ugaguugguu uagccaaaua uuaauaggaa ccuuacugau gugguuagga     2040

cuuaacacga aaaauggaag cauaucacuu augugucucg ccuugggagg aguauuaaua     2100

uuucuaucga cggcaguaag cggcggggua gguuguucag uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9240
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 3 opt

<400>  9240
gggagaaagc uuaccaugag aauaauaaac gcaagaaaag aaaaaaaaag gagagguaca       60

gauacauccg ucggaauagu ggguuuauua cuuacaacug caauggcggc agaaguaaca      120

cgaagaggaa gcgcauauua uauguauuua gaucgaagug augcgggaga agcaauaagc      180

uucccaacaa cauuaggaau gaacaaaugu uauauacaaa uaauggauuu aggacacaug      240

ugugaugcaa cgaugucaua ugaaugucca auguuagaug aaggaguaga accggaugau      300

gucgauuguu gguguaauac aacaucaaca uggguaguau auggaaccug ccaucauaaa      360

aaaggugaag caagaagauc uaggagagca guaacgcuuc caagucauag uacaaggaaa      420

uuacaaacua gaagucaaac augguuagaa aguagagaau acacuaaaca uuuaauacga      480

guagaaaacu ggauauuuag aaauccaggc uucgcauuag ccgcagcugc cauagcaugg      540

uuauuagguu cgaguacguc acaaaaaguu auauauuuag uuaugauucu auuaauugcc      600

cccgcauaua guauaagaug cauaggagug aguaaccggg auuuuguuga aggaauguca      660

ggaggaacau gggucgaugu uguauuagaa caugguggau gcguaacagu uauggcacaa      720

gacaaaccaa caguggacau agaauuagua acaacaacgg uaaguaauau ggcagaagua      780

cgaaguuacu guuaugaagc aaguauauca gauauggcaa gcgauucuag auguccaacu      840

caaggcgaag cauaucuaga uaaacaaucc gauacacaau acgugugcaa gaggacguug      900

guagauagag gaugggguaa ugggugcggg uuauucggaa aagguucauu aguaaccugc      960

gcuaaauucg cguguucuaa aaaaaugaca ggaaaaucaa uacaaccgga aaauuuagag     1020

uauagaauaa uguugucagu ucauggaagu caacauucag gaaugauagu aaaugauacu     1080

ggacaugaaa cagacgagaa ccgagcaaaa guugaaauaa caccaaauag uccaagggca     1140

gaagcuacau uaggaggauu uggcucucua ggacuggauu gugaaccaag aacuggcuua     1200

gauuuuagcg accuauauua ccuaacgaug aauaauaagc acuggcuagu acacaaagaa     1260

ugguuccacg auauaccauu accauggcau gcaggugcag auacaggaac uccacauugg     1320

aauaauaaag aagcacuagu ggaguuuaaa gacgcacaug caaaaaggca aacaguugua     1380

guuuuaggca gucaagaagg cgcuguacau acagcauuag caggagcacu agaagcggaa     1440

auggauggag caaaaggaag auuaucaagc ggacaucuaa aauguagguu aaaaauggau     1500

aagcuuagau uaaaaggagu caguuacuca uuauguacgg cagcuuuuac cuuuagcaaa     1560

auaccagcag aaacacuaca cggcacaguu acugaagaag ugcaauaugc uggaacugau     1620

ggcccaugca aaguaccggc acaaauggca guagauaugc aaacacuaac accaguagga     1680

agauuaauaa cagcaaaccc aguaauaaca gaaucaacag aaaacaguaa aaugauguua     1740

gaauuagacc caccauuugg agauucauau auagucauag gaguaggaga aaaaaaaauu     1800

acccaucauu ggcauagaag ugguaguaca auaggcaaag cauucgaagc uacaguaaga     1860

ggagcaaaaa ggauggcagu cuuaggagac acagcauggg auuuuggauc aguaggagga     1920

gcuuuaaaua guuuaggaaa aggaauucau cagauauucg gagcagcauu uaaaagccua     1980

uucgguggaa ugagcugguu uucgcaaaua uugauaggaa cauuacuaau gugguuagga     2040

uuaaacacaa aaaacggauc uauaagucug augugccuag cguuaggggg cguucuaaua     2100

uuccucucaa cagcaguauc agcggacgua ggauguucag uagauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9241
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 3 opt

<400>  9241
gggagaaagc uuaccaugag aauaauaaau gcacgaaaag aaaaaaaaag aagaggaaca       60

gacacuucag uaggcauugu aggacuacua cuuacaacag caauggcagc agagguaaca      120

cgaaggggaa gugcauauua uauguauuua gacagaucag augcaggaga agcgaucuca      180

uuuccuacaa cauugggaau gaauaaaugu uacauccaaa uaauggaucu gggccauaug      240

ugugaugcaa caaugaguua cgaaugucca auguuagaug aaggggucga gccagaugau      300

guagauuguu gguguaauac cacuucaaca uggguuguuu auggaacaug ccaucauaaa      360

aaaggagaag caagaagauc cagaagggca guaacauuac caucacauuc uacacgaaaa      420

uuacaaaccc gaucgcaaac auggcuagaa aguagagaau acaccaaaca uuuaauaaga      480

gucgaaaauu ggauauucag aaacccaggc uuugcuuuag cagcagcugc gauagccugg      540

cuacuaggaa guagcacuuc acaaaaaguc aucuauuuag uaaugauuuu acuaaucgcu      600

ccagcauaca gcauuagaug uauuggaguu aguaauagag acuuuguaga aggaauguca      660

ggcggaacau ggguugaugu gguccuagaa cauggcggau guguaacagu aauggcccaa      720

gauaaaccca caguagauau agaauuagua acgacaacag ugagcaauau ggcagaagua      780

agaucauauu gcuacgaagc uucaauaucu gauauggcaa gcgauucgag augcccaaca      840

caaggagaag cauaucucga uaaacaaagc gacacucaau auguaugcaa aagaacacua      900

guagacagag gcugggguaa ugguuguggu uuguuuggaa aagggaguuu aguaacaugu      960

gcuaaauucg caugcucgaa gaaaaugaca ggcaaaucaa uacaaccuga aaauuuagaa     1020

uacagaauaa ugcuaagugu acaugguuca caacauucag guaugauugu aaaugauaca     1080

ggucacgaaa cagaugaaaa uagagcuaaa guagaaauaa cccccaauuc accaagagcu     1140

gaagccacac uaggaggcuu cgguaguuua ggguuagauu gugaaccuag aacagggcuu     1200

gauuuuucag accuuuauua ucucacaaug aauaauaaac acuggcucgu acauaaagaa     1260

ugguuccaug acauaccucu acccuggcac gcuggagcag auacaggaac gccacacugg     1320

aauaauaaag aagcuuuagu agaauucaaa gaugcccacg ccaaaagaca aacuguagua     1380

guacuaggaa gucaagaagg agcaguacac acagcuuuag caggagcauu agaagcggaa     1440

auggacggag cgaaaggaag auuaucaucu ggucauuuaa aauguagauu gaaaauggau     1500

aaacuaagau uaaaaggugu uucauauagc uuaugcacag cugcauuuac auuuacaaaa     1560

auaccagcag aaacuuuaca uggcacagua acaguagaag uccaguaugc agguacugau     1620

ggaccuugua agguacccgc acaaauggca guagauaugc aaacacucac accaguagga     1680

agacuaauua cagcuaaucc aguuauuaca gaaagcacag aaaauuccaa aaugaugcua     1740

gaguuagauc caccuuuugg ugacaguuau auagugauag gggucggaga aaaaaaaaua     1800

acacaucacu ggcauagaag cggaucaaca auaggaaaag cauuugaagc cacaguaaga     1860

ggagcuaaaa gaauggcugu acuaggcgau acagcauggg auuucggaag uguuggcgga     1920

gcucugaaca gccucgggaa agguauacau caaauauuug gugcggcauu uaaaaguuua     1980

uuuggaggaa ugucaugguu uucacaaaua uuaauaggaa cacuacuaau gugguuaggu     2040

cucaauacua aaaauggaag uauaagcuua auguguuuag cauugggagg agucuuaaua     2100

uuccuaucaa cagcaguaag ugcagaugua ggauguagug uugauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9242
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 3 opt

<400>  9242
gggagaaagc uuaccaugag aaucauuaau gcacguaaag aaaagaaaag aaggggaaca       60

gauacgucug uaggaauagu uggacuacua uuaacaacug caauggcugu agaaguaaca      120

agaagaggaa augccuauua uauguauuua gauaggucgg acgcaggaga agcaauuuca      180

uucccaacaa caaugggaau gaacaaaugu uauauccaaa ucauggaccu uggacauaug      240

ugugacgcaa caaugucaua ugaaugucca auguuagaug aagggguuga accagaugau      300

guagauuguu ggugcaacac aacaagcaca uggguagucu acggaacaug ucaucauaag      360

aaaggagaag cacgaagauc aagacgagca guuacauuac caagucacuc aacaagaaaa      420

uuacaaacaa gaucacaaac auggcucgaa aguagagaau acaccaaaca ucugauccga      480

guagaaaacu ggauauuuag aaaucccggu uuugcucuug cugccgccgc aaucgcaugg      540

uuacuaggau cgucuacuuc acaaaaaguu auauauuuag uaaugauauu auugauagcu      600

ccagcauauu ccauuagaug cauaggaguu uccaauagag auuuuguaga agggaugagc      660

ggagguaccu ggguagaugu aguauuagaa cacggaggau gcguuacagu aauggcacaa      720

gacaaaccaa caguagacau agaauuaguu acaacaacag uaucaaacau ggcagaaguu      780

cguuccuauu gcuacgaagc aaguauuuca gauauggcaa gugauaguag auguccgaca      840

caaggagaag cauauuuaga caaacaauca gacacucaau auguauguaa aagaacucua      900

guagauagag guuggggaaa uggcugugga uuauucggca aaggaucucu aguaacaugu      960

gcaaaauuug ccuguaguaa gaaaaugaca ggaaaaucca uccaaccaga aaauuuagaa     1020

uaucgaauaa uguuaucagu acauggauca caacauuccg gaaugaucgu caacgauacu     1080

ggucaugaga cugaugaaaa uagagcaaaa guagaaauaa caccaaauuc accucgagcu     1140

gaagcaacau ugggaggauu uggaucacua ggacuagacu gugaaccaag aacaggacuu     1200

gauuuuagcg acuuauauua ccuuacgaug aacaauaaac auugguuagu acauaaagaa     1260

ugguuucacg acauaccacu accuuggcau gcaggagcag auacgggaac uccccauugg     1320

aacaauaaag aggcuuuagu agaguucaaa gacgcacacg caaaaagaca aacaguagua     1380

guucuuggaa gucaagaagg agcuguacac acagccuuag cuggugcacu agaagcagaa     1440

auggauggag caaaaggaag auuaaguagu ggacacuuaa aauguagauu aaaaauggau     1500

aaauuacgau uaaaaggagu uucauauuca uuaugcacag cagcuuuuac guucacgaag     1560

auaccagcag aaacacugca cggaacagua acaguagaag uacaauaugc cgguacagau     1620

ggaccuugua agguaccagc acaaauggcu guugacaugc aaacauuaac ucccguuggu     1680

agacuaauaa cagcaaaucc aguaauaacu gaaagcacag aaaauucuaa aaugaugcua     1740

gaauuagauc caccuuuugg agauagcuac aucguaauag gaguuggcga aaagaaaaua     1800

acucaucacu ggcaccguuc agggucaacc auugguaaag cauuugaagc aacaguaaga     1860

ggagccaaaa gaauggcggu acuaggagau acggcauggg auuuuggaag uguaggaggu     1920

gcccuaaauu ccuuggguaa aggaauacac caaaucuuug gagccgcauu uaaaucauua     1980

uucgggggaa ugaguugguu cagccaaaua uuaauuggaa cacuauuagu augguuagga     2040

uugaauacua aaaauggauc uauaucacua augugccuag cauuaggagg agugcucaua     2100

uuccuaagua cugcuguuuc agcagaugua ggauguagug uagauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9243
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 3 opt

<400>  9243
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaaaaaacg aagaggaaca       60

gauacaagcg uggguauagu uggcuugcua uugacuacug caauggcagc ugaaguaacc      120

agaagaggaa gugcauacua cauguaucua gaucguucug augccgguga agcaaucuca      180

uuuccuacaa cauuagguau gaacaaaugu uauauacaaa uaauggaucu aggucauaug      240

ugugaugcaa caaugucuua ugaauguccu augcuggacg agggaguuga accagaugac      300

gucgauugcu ggugcaauac cacguccaca uggguaguuu auggaacuug ucaccacaaa      360

aaaggagaag caagaagaag uagaagagca guaacgcuac caagucauag uacaagaaaa      420

cuccaaacaa ggucacagac uuggcucgaa aguagggaau auacuaaaca ucuaauacga      480

guagaaaauu ggauauuuag aaauccaggg uuugcguuag cagcagcagc aauagcuugg      540

uuauuaggcu ccaguacuuc acaaaaagua aucuacuuag uaaugauauu auuaauagcc      600

ccagcauauu caauaagaug uauagguguu aguaauagag auuuuguaga aggaauguca      660

ggagguacuu ggguagaugu cguucuagaa cacggaggau gcguaacagu aauggcacaa      720

gauaaaccca cuguagauau agaacuaguu acaacaacug uauccaauau ggcagaagua      780

agaaguuauu guuacgaagc uagcauaagc gauauggcaa gugacucaag augucccaca      840

caaggugaag cauaccuuga uaaacaauca gauacucaau auguaugcaa acguacacug      900

guagaccgug gaugggggaa uggaugcgga cuauucggua aaggauccuu aguuacaugu      960

gcaaaauuug cuuguucaaa aaaaaugacu gggaaaucaa uacaaccaga aaacuuagaa     1020

uauagaauua uguuaucggu acacggauca cagcauucug gaaugauagu aaaugauaca     1080

ggacaugaaa cagaugaaaa ucgagcuaaa guagaaauaa cuccuaauag uccaagagcg     1140

gaagcaacuc uaggagguuu uggaagucua ggauuagauu gcgaaccgag aacuggguua     1200

gacuuuucag auuuauauua ucuaacuaug aacaacaagc acugguuagu acauaaagag     1260

ugguuucaug auauaccgcu accauggcau gcgggagcug auacaggaac uccacauugg     1320

aauaauaaag aagcacuagu ggaauuuaaa gaugcacaug caaaacgaca aaccguggua     1380

guacuaggau cacaagaagg agcaguacau acagccuuag cuggugcauu agaagcagaa     1440

auggauggag caaaaggaag auuaaguagu ggucaucuaa aauguagacu aaagauggac     1500

aaauuaagau uaaagggagu gucuuacagu cuauguacag cagccuuuac auuuacaaaa     1560

auaccugcug aaacauuaca cggaacagua acuguggaag uacaauaugc agguaccgau     1620

ggaccaugua aaguuccugc acaaauggcu guagauaugc aaacucucac accgguagga     1680

cgacuaauaa cagcaaaucc aguaaucaca gaaaguacug aaaauaguaa aaugaugcuc     1740

gaguuagauc cacccuuugg cgacagcuau auaguaaucg gaguagguga aaaaaaaauu     1800

acacaccacu ggcacaggag uggaagcaca auaggaaaag cuuucgaagc aacaguuaga     1860

ggugcaaaaa gaauggcugu acugggagau acagcauggg acuuuggaag uguaggagga     1920

gcacuaaaua gcuuaggaaa aggaauacac caaauauuug gagcagcauu uaaaucacua     1980

uuuggaggua ugaguugguu uucgcaaauu uuaauaggaa cauuauuaau gugguuaggg     2040

cuaaauacaa aaaauggaag uauaucauua guuugucuag cgcucggagg aguacuuaua     2100

uuuuuaagca cagcaguauc agcagacguu ggauguagug uggauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9244
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 3 opt

<400>  9244
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaaaaaaaag aagaggaaca       60

gacacaagug uuggaauugu ugguuuacua cuuacgacag cuauggcagu agagguuaca      120

aggagaggua gugcauauua uauguaccua gauagaucag augcaggaga agccauauca      180

uuuccgacca cuuuaggaau gaauaaaugu uauauacaaa uaauggaucu aggacauaug      240

ugugaugcaa caaugaguua cgaaugccca auguuagaug aaggaguaga accugaugau      300

guagauugcu gguguaacac uaccucaaca uggguuguau acggaacaug ucaucauaaa      360

aaaggugaag cuagacgaag cagacgagcu guaacguuac caagucauuc aacacgaaaa      420

cuacaaacua gaucacagac auggcuugaa ucaagagaau auacaaaaca cuuaauuaga      480

guugaaaauu ggauauuuag aaauccagga uuugcacucg cagcagcagc aauugcaugg      540

cuccuuggca guaguacauc acaaaaagua auauauuuag uuaugauauu auuaauagcu      600

ccagcauaca guauucgaug cauaggaguu ucaaacagag acuuuguaga aggaauguca      660

ggaggcacau ggguagacgu aguacuagaa cacggcggau guguuacagu uauggcccaa      720

gauaaaccag ccguagauau ugaauuaguu acuacgacag uaaguaauau ggcagaagug      780

agaucauauu guuaugaagc aaguauauca gauauggcgu cagauagccg uugccccacc      840

caaggcgaag cuuaucuaga caaacaaagu gacacacagu acguauguaa aagaacauua      900

guagacaggg gauggggaaa uggaugcgga cuauuuggca aaggaagcuu aguaacaugc      960

gcaaaauucg cuuguagcaa aaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa     1020

uauagaauua uguugucagu acacgggagu caacauuccg gaaugauagu aaaugacacu     1080

ggucacgaaa cagacgaaaa uagagcaaaa guagaaauaa cgccaaauuc uccuagagca     1140

gaagcaacac uaggaggauu ugggucuuua ggguuagacu gugagccaag aacaggauua     1200

gauuuuucug auuuauauua uuuaacaaug aauaacaaac auugguuagu acauaaagag     1260

ugguuccaug acauaccucu accauggcau gcaggagcag auacuggaac uccacacugg     1320

aauaacaaag aagcacuugu ugaauuuaaa gacgcucaug caaaaagaca aaccguaguu     1380

guauuaggau cacaagaagg ugcggugcac acagcuuuag caggggcccu agaagccgag     1440

auggauggug caaaagguag acuuagcucu ggucaccuua aaugccgacu aaaaauggau     1500

aaauuaagac uaaagggagu aaguuauagc cuauguaccg cagcguuuac auuuacaaaa     1560

auaccagcag aaacacuaca uggaaccgug acaguagaag uucaauacgc aggaacagau     1620

gguccaugua aaguaccagc acaaauggca guagauaugc aaacauuaac accaguggga     1680

agauuaauaa cagcaaaccc aguaauaaca gaaaguacag aaaauucaaa aaugauguua     1740

gaauuagacc ccccuuuugg agauagcuau auagucauag guguuggcga aaaaaaaaua     1800

acacaucauu ggcauagaag cgguucaaca auaggaaagg cauuugaagc aacgguacga     1860

ggugcgaaaa ggauggcugu auuaggagau acggcauggg auuuuggauc aguaggagga     1920

gcguuaaauu cauuaggcaa aggaauacau caaauauucg gagcugcguu uaaaucauua     1980

uuugguggua ugucaugguu cagucaaaua uuaauaggaa cacuuuuagu cuggcuaggu     2040

uuaaacacca aaaaugguag cauaucauua acaugcuuag cauuaggagg aguauuaaua     2100

uuccucagca cagcgguuuc agcagacgua ggauguagug uagauuuuag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9245
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 3 opt

<400>  9245
gggagaaagc uuaccaugag aauaauaaau gccagaaaag aaaaaaaaag aagaggagcg       60

gacacuucag uagguauagu aggacuauua uuaacaacag cgauggccgu agaaguaaca      120

agaagaggaa gugcauauua uauguacuua gauagaagcg acgcaggaga agcgaucagu      180

uucccaacaa cauuagguau gaauaaaugu uauauacaaa uaauggaucu uggacauaug      240

ugugacgcaa cuaugucaua cgaaugucca augcuagacg aaggaguaga accugaugac      300

gucgauuguu gguguaauac aacuagcaca uggguaguau auggaacaug ucaucauaaa      360

aagggugaag cuagaagaag uagaagagca guaacgcuuc caucacacag uacaagaaaa      420

uuacaaacua gaagucaaac auggcuagaa ucuagagaau auacuaaaca ucucauacga      480

guagaaaacu ggauauuucg uaauccggga uuugcauugg cugcggcugu aauagccugg      540

uuauuaggaa guaguacaag ucaaaaagug auauauuuag uaaugauacu acuuauugcu      600

ccagcuuaca guauaagaug caucggagua ucaaauagag auuucgucga aggaaugagu      660

ggagggacau ggguagacgu aguacuagaa cauggaggcu gcguaaccgu aauggcacaa      720

gauaaaccaa caguagauau agaauuagua acaacgacag uuuccaauau ggcugaagua      780

cgaucauacu guuaugaagc cagcauuagu gacauggcau cugauucuag gugucccacu      840

caaggugagg cauaucuaga caaacaauca gauacacaau auguauguaa aagaacauua      900

guagauagag gaugggggaa uggauguggg cuauuuggaa aaggaucguu agucacaugc      960

gcgaaauuug cuuguucaaa aaaaaugaca ggcaaaucaa uucaaccuga gaauuuagaa     1020

uauagaauca uguuaucagu ucauggauca caacacagcg gaaugauagu aaaugauacg     1080

ggacaugaaa cugacgaaaa uagagcaaaa guugaaauaa cuccaaauuc cccuagagca     1140

gaagcaacac uagguggauu uggaucauua ggacuagauu gcgaaccaag aacaggauua     1200

gauuuuagcg aucuauacua uuuaacaaug aauaacaaac auugguuagu acauaaagaa     1260

ugguuucaug auauaccauu accauggcau gcaggggcag acacugguac accgcauugg     1320

aauaauaaag aagccuuagu agaauuuaaa gacgcacaug caaaaagaca aacaguuguc     1380

guauuagguu cucaagaagg agcaguacau acggcuuuag caggagcucu cgaagcugag     1440

auggacggag caaaaggcag acuuucaagu ggacaucuca aauguagguu aaagauggau     1500

aaacuucgau uaaaaggagu aaguuacucc cuauguacug cagcauuuac uuuuacaaaa     1560

auaccagcug aaacuuugca uggaacagua acaguagaag uacaauacgc aggaacggac     1620

ggaccaugca aaguuccagc acaaauggca guagauaugc aaacacugac gccaguagga     1680

agguuaauua cagcgaaccc aguaauuaca gaaucuaccg aaaauucaaa aaugauguua     1740

gaauuagacc ccccuuuugg agacucuuac auaguuaucg gaguagguga aaaaaaaaua     1800

acacaccauu ggcaucguag ugguuccaca auaggaaaag cuuucgaagc aacuguaagg     1860

ggagcaaaaa gaauggcagu uuuaggagac acggcauggg acuucggaag uguaggaggg     1920

gcacugaauu cucuaggaaa aggcauacau caaauauuug gugccgcauu uaaaucauua     1980

uuuggaggca ugaguugguu uucucaaaua uuaauuggua cauuauuagu auggcuagga     2040

uugaauacca aaaacggauc cauaucacua acaugucuag cacuuggagg aguucuaauc     2100

uuucuaucaa cagcuguauc agcagaugua ggcuguucug uagauuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9246
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 3 opt

<400>  9246
gggagaaagc uuaccaugag aauuauuaau gcaagaaaag agaaaaaaag aagaggagca       60

gauacaagug uuggaauagu aggauuacua cuaacaacag caauggcagu ggaaguaaca      120

cgcagaggau cugcauauua uauguaucua gauagaagug augcuggaga agcaauaagu      180

uuuccgacaa cucuaggagu aaacaaaugu uauauucaaa uaauggaucu aggacacaug      240

ugcgaugcaa cuaugucaua cgaguguccu auguuagaug aaggugucga accugaugau      300

guagacuguu gguguaauac aacauccacg uggguagucu auggcacaug ucaucauaaa      360

aaaggagaag cuagacgauc aagaagagca guuacucuac caagccacuc gacaagaaaa      420

cuacaaacaa gaucacaaac auggcuugaa ucaagagaau auacaaaaca ucuaauaaga      480

gucgaaaauu ggauauucag aaauccagga uuugcauuag cagccgcagc aauagcaugg      540

uuauuaggcu caaguacaag ccaaaaagua auauaucuag uaaugauacu cuuaauagca      600

ccagcauacu cuauaagaug cauugguguu aguaacagag acuuugucga aggaaugucg      660

ggaggaacuu ggguagaugu aguacuagaa caugguggau guguaacagu aauggcacaa      720

gauaaaccaa caguagauau agaauuaguu acaacaacgg uaucaaauau ggcagaaguu      780

agaagcuacu guuaugaagc aaguauuuca gacauggcau cagacucaag auguccuaca      840

caaggagaag cauaucuuga uaaacaaagc gauacacagu acguauguaa aagaacacua      900

guugauagag gaugggguaa uggauguggu cuauuuggaa aaggaaguuu aguaacuugu      960

gcaaaauuug cauguucuaa aaaaaugaca ggaaaaucaa uucaaccuga aaacuuagaa     1020

uauagaauaa uguuaucugu acauggaucc caacauucag gaaugauugu aaaugauaca     1080

ggucaugaaa cagaugaaaa uagagcuaaa guagaaauca cuccgaacuc accacgagca     1140

gaagcuacuu uaggaggauu cgggucauua ggccuugauu gcgaaccaag gaccggacua     1200

gauuuuucag auuuauacua cuuaacuaug aauaauaaac acugguuagu acacaaggaa     1260

ugguuucaug acauacccuu accauggcau gcaggagccg acacaggaac uccacauugg     1320

aauaauaaag aagcacuagu agaauucaaa gaugcucaug ccaaaagaca aacaguagua     1380

guuuuaggaa gccaagaagg cgcuguccau acagccuuag caggagcacu cgaagcagaa     1440

auggauggag ccaaaggaag acuaucauca ggucauuuaa aaugucgucu uaaaauggau     1500

aaauuaagac uuaaaggugu uaguuauagc uuauguacag cggcauuuac cuuuacaaaa     1560

auaccugccg aaacacuuca cggaacaguu acaguugaag uacaauaugc agggacagac     1620

ggaccaugca aaguuccugc acaaauggca guugauaugc aaacauuaac accaguaggc     1680

agauuaauaa cagcaaaccc aguuauaacu gaaucaaccg aaaacaguaa aaugauguua     1740

gaauuagauc caccuuuugg cgacaguuau auaguaauag gaguagguga caaaaaaaua     1800

acucaucacu gguacagaag uggaucaacu auagggaaag cauuugaagc aacaguaaga     1860

ggggcaaaaa gaauggccgu auugggagau acggcauggg acuuuggcuc aguaggcggu     1920

gcuuugaaua guuuaggaaa agguauacau caaauauuug gagcagcauu uaaaucauug     1980

uuuggaggaa ugaguugguu uucucaaauu uugauuggua cauuauuagu gugguuagga     2040

cuaaauacca aaaacggaag uaucagccua acuugcuuag cccuaggggg gguacuaaua     2100

uuuuuaagua cagcagugag ugcggacgua ggaugcagug ucgacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9247
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 3 opt

<400>  9247
gggagaaagc uuaccaugag gauaaucaau gcaagaaaag aaaaaaaaag aagaggugcu       60

gacacauccg ucggaauagu aggacuguua cuaaccaccg cgauggcugu agaaguaaca      120

cgacgcggca gcgcauauua uauguacuua gauagaagug augcagguaa agcaaucucc      180

uuuccaacaa cauuaggagu aaauaaaugu uacauacaaa uaauggauuu aggacauaug      240

ugugaugcaa caaugucaua ugaauguccu augcuggaug aaggaguaga accugaugac      300

guugauuguu ggugcaauac cacuucaaca ugggucguau auggaacaug ucaucauaaa      360

aaaggagaag cuagaagaag cagaagagcg guuacucuuc caagucauag uacuagaaaa      420

cuacaaacaa gaucucaaac uuggcuugaa ucaagggaau acaccaaaca uuuaauaaga      480

guagaaaauu ggaucuucag aaauccagga uucgcacuag cagcagcggc aauagcaugg      540

cuacuugguu caucuacaag ccaaaaagua auauauuuag ucaugauacu auuaauagca      600

cccgcauauu cuaucagaug cauaggagua aguaauagag auuuuguaga gggaaugucc      660

ggaggaacau ggguagaugu aguccucgaa caugguggau guguuacagu gauggcacaa      720

gacaagccaa caguagauau agaauuaguu acgacaacug ucucaaauau ggcagaagua      780

agaagcuauu guuaugaagc aagcauaucu gauauggcaa gcgauucaag auguccuacu      840

caaggagaag cguauuuaga uaaacaguca gacacacagu acguauguaa acgaacacua      900

guagacagag gauggggaaa cggaugugga uuauucggaa aagguaguuu aguaaccugu      960

gcaaaauuug cguguucaaa aaaaaugacu ggaaaaagua uacaaccaga aaauuuagaa     1020

uauaggauaa ugcucagcgu gcauggauca caacauucag gaaugauagu aaacgauaua     1080

ggacaugaaa cagacgaaaa ucgagcaaaa gucgagauaa cacccaauuc accacgagca     1140

gaggcuaccc uuggagguuu cggaucauua ggauuggauu gcgaaccacg aacaggacuc     1200

gauuuuagug auuuauauua ucuuacaaug aacaacaaac auugguuagu acauaaagaa     1260

ugguuucaug acauuccacu accauggcau gcaggagcag auacugguac accacacugg     1320

aacaauaaag aagcacuagu agaauuuaaa gaugcucacg cuaaaagaca aacaguagug     1380

guauuaggaa gucaagaagg agcaguacau acagcacuag caggagcccu ggaagccgaa     1440

auggauggag caaaaggaag gcuaucauca ggccaucuaa aaugcagacu aaaaauggau     1500

aaauugagau uaaaaggagu aucguauagc uuaugcacag cagcguuuac uuuuacaaaa     1560

auaccagcug aaacacuaca cggaaccgua acuguagagg uacaauacgc aggaacagau     1620

gguccaugua aagucccagc acaaauggca guagauaugc aaacauuaac acccguagga     1680

aggcuuauaa cugcaaaucc aguuauaaca gaaaguacag aaaauucuaa aaugaugcug     1740

gaauuagauc cacccuucgg agauucauac auaguaauag gaguaggaga uaaaaaaauu     1800

acacaucauu ggcauagauc ugggaguaca auugguaaag ccuuugaagc aacagugcga     1860

ggcgcaaaaa gaauggcagu auuaggagau acagcuuggg auuuuggauc agugggagga     1920

gcauuaaaua guuuaggaaa aggaauacau caaauauucg gagcagcauu uaaaagucua     1980

uuuggaggca ugucaugguu uucacaaauu cuuauaggaa cgcuuuuagu augguuagga     2040

uuaaauacaa aaaauggauc aauaucacug acauguuuag cgcuaggugg cguuuuaaua     2100

uuuuuaagca cggcaguauc agcagacgua ggaugcucag uagauuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9248
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 3 opt

<400>  9248
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaagaaaaag aagaggagca       60

gauacaagca uagguauagu agggcuauua uuaacaacug cuauggcagc agaaaucacg      120

agaagaggaa gugcauauua cauguauuug gacagaucug augcaggaaa agcaauaucu      180

uucgcaacaa cuuuaggcgu aaacaaaugu cauguucaaa uaauggacuu aggccacaug      240

ugugaugcga caaugaguua ugaaugucca auguuagaug aaggaguaga gccagaugau      300

guugauuguu ggugcaauac aacaagcaca ugggucguuu acgggacuug ucaucauaag      360

aaaggagaag caagaagaag uagacgagcu guuacuuuac caagccauag uacaagaaaa      420

uugcagacaa gaagccaaac auggcucgaa agcagagaau auacaaaaca uuuaauaaaa      480

guagaaaauu ggauauuccg aaacccagga uuuacgcugg uagcaguagc cauagcuugg      540

uuguuaggau ccuccacguc acaaaaagug auauacuuag ugaugauauu acuaauugca      600

ccagcuuauu caauaaggug uauaggagua ucaaauagag auuuuguaga aggaaugagu      660

ggaggaacuu ggguagacgu aguacuagaa cauggagguu gugugacugu aauggcacaa      720

gauaaaccga cuguugauau agaacuugua acaacaacug uaucaaauau ggcugaagua      780

agaucauacu gcuaugaagc aucuauaagu gauauggccu ccgauucaag gugcccaaca      840

caaggggagg cuuacuuaga uaaacaaagu gauacacaau auguauguaa acgaacauua      900

guagauagag gaugggguaa ugguugcgga uuauuuggaa aaggaaguuu gguaacaugu      960

gcuaaauuua cuugcaguaa gaaaaugaca ggcaaaagca uacaaccgga aaaucuggaa     1020

uauagaauaa uguuaucagu acauggauca caacauagug gaaugauagu aaaugacacu     1080

gggcaugaaa cagaugaaaa uagagcaaaa guagaaguua caccgaacag cccaagagcg     1140

gaagcaacuu uagguggguu cggaucccua ggacuagacu gugaaccaag aacaggcuug     1200

gauuuuagug acuuauauua ccuaacaaug aacaauaaac auuggcuagu ucauaaagaa     1260

ugguuucacg auauaccauu accauggcac gcaggagcag auacaggaac accacauugg     1320

aauaauaaag aagcgcuagu cgaguucaaa gaugcacacg caaaacgaca aacaguaguc     1380

guacuugguu cacaagaagg agcaguucac acugcuuuag cgggugcauu agaagcagaa     1440

auggauggag caaaaggaag acuauuuuca ggacaucuua aaugccgucu uaaaauggau     1500

aaauuaagac uuaaaggagu aaguuauuca cuaugcacag cagcauucac auuuacuaaa     1560

guuccugcag aaacauuaca cggaacagug acaguugaag uccaauacgc uggaacagau     1620

gguccaugua aggucccagc acaaauggcg guagauaugc aaacauugac accaguugga     1680

agacuaauua cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugauguua     1740

gaauuggacc cacccuuugg agauaguuau auaguuauag gaguaggaga uaagaaaaua     1800

acacaucacu ggcauagauc uggaucaacg auaggaaaag cauuugaagc aacuguaaga     1860

ggagcuaaaa gaauggcagu acuuggagau acagcauggg acuucggcuc aguaggcggu     1920

gucuucaauu cauuaggaaa aggaauacau caaauauuug gcgcagcauu uaaaucacua     1980

uucggaggua ugaguugguu uucacaaaua cuuauaggaa cccuuuuagu uuggcuagga     2040

cuaaauacua aaaaugguuc aaucaguuua acauguuugg cauuaggagg aguuaugauc     2100

uuucuaagca cagcuguuag ugcugaugua ggguguagcg uagauuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9249
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 3 opt

<400>  9249
gggagaaagc uuaccaugag aauuauaaau gcaagaaaag agagaaagag acgaggagcc       60

gacacaucua uaggaauaau aggacuacua uuaacaaccg caauggcagc agaaauaacu      120

cgaagaggga gugcauacua cauguauuua gaucgaucag acgcugguaa agccauaagu      180

uuugcgacua auuuaggagu aaacaaaugc cauguucaaa uuauggaccu aggacauaug      240

ugcgaugcaa caaugaguua cgaaugucca augcuagaug aaggaguaga accagacgau      300

guagauugcu gguguaauac aacaucaaca uggguuguau acggaacaug ucaucauaaa      360

aaaggagaag caagacgauc aagaagagca guaacuuugc ccucucacag cacaagaaaa      420

uuacagaccc gaagccaaac cuggcuagaa ucaagagaau auacaaaaca ucuuauaaaa      480

guugaaaauu ggauauuuag aaaucccggu uuugcguuag cagcaguagc uauagccugg      540

uuacuuggaa gcaguacauc acaaaaaguu auauauuuag uuaugauauu auuaauugcg      600

ccagcuuauu caauuagaug uaucggagua agcaaccggg auuucguuga gggaauguca      660

ggggguacau ggguagaugu aguauuagaa caugggggau gcguaacagu aauggcgcaa      720

gauaaaccaa caguagacau agaauuagua acaacgaccg ugucaaacau ggcagaaguu      780

cgcucauauu guuaugaagc aaguauaucu gauauggcau cagacucaag auguccuaca      840

caaggugaag cauacuuaga caaacaaucu gauacucagu acgucuguaa acgaacucua      900

guagacagag gauggggaaa uggaugcggu cuauuuggua aaggaucacu agugacuugu      960

gcaaaauuua cauguagcaa aaaaaugaca gguaaaagua uucagcccga aaaccuugag     1020

uauagaauaa uguuaucagu ccauggaucu caacauagug guaugauagu caaugauaua     1080

ggccaugaaa cagaugaaaa uagagcaaaa guugaaguaa caccaaauuc accaagagca     1140

gaagcaaccc uagggggauu uggaucacuu ggauuagauu gugaaccuag aacuggacua     1200

gacuuuucag acuuauacua uuuaacaaug aacaauaaac auugguuggu acauaaagaa     1260

ugguuucacg auaucccuuu acccuggcau gccggagccg auacaggaac cccacauugg     1320

aauaauaaag aggcacuagu ugaauuuaag gaugcacaug cgaaaagaca aacaguagua     1380

guacuaggau cacaagaggg agccguacau acggccuuag cuggagcacu agaagcagaa     1440

auggacggag cuaaaggaag acuauuuagc gggcacuuga aaugccgauu aaaaauggau     1500

aaauuacgac uuaaaggagu uuccuacuca uuaugcacag cugcauuuac guuuacuaaa     1560

guaccugcag aaacauugca cggaacagua acaguagaag uacaauaugc ugguacagau     1620

ggacccugua aagugccagc gcaaauggcu guggauaugc aaacauuaac uccuguugga     1680

agacuaauaa cagcaaaucc aguaauaaca gaaucaacug aaaacaguaa aaugauguua     1740

gaauuagacc cuccauuugg ggauucguau auaguaauag guguuggaga uaaaaaaaua     1800

acacaucauu ggcauagaag ugggucgaca auaggaaaag cuuucgaagc aacaguaaga     1860

ggagcaaaaa gaauggcagu cuuaggagac acagcauggg auuuuggauc aguuggaggu     1920

gucuucaaua guuuagguaa aggaguacac caaaucuucg gagcagcuuu caaaagucua     1980

uuugggggaa ugucaugguu uucacaaaua cuuauuggaa cauuacuagu cugguuagga     2040

cucaauacaa aaaauggcag uauaagcuua acauguuugg cauuaggagg cgugaugauu     2100

uuccuaagca cagcaguauc agcagacgua gguuguagug uugauuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9250
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 3 opt

<400>  9250
gggagaaagc uuaccaugcg aauaauaaac gcaagaaaag aacgaaaaag aagaggagca       60

gacacaucaa uuggaauaau aggucuguua uuaacaacag ccauggccgc agaaauuaca      120

agaagaggca gugcauauua uauguauuua gaccgaagug augcuggaaa agcaauauca      180

uucgcuacua cauugggagu caauaaaugu cauguccaaa uuauggaccu uggucauaug      240

ugcgacgcaa cuaugucaua ugaauguccg auguuagaug aggguguuga accagacgac      300

guagauuguu ggugcaacac aacaaguacu uggguaguau auggaacaug ccaucauaaa      360

aaaggagagg cacgccgaag uagaagagca guaacacuac caucucacuc aacaagaaaa      420

uuacaaacua ggucacaaac augguuagag ucacgcgaau acacaaaaca uuuaauaaaa      480

guagaaaauu ggauauuuag aaauccagga uuugcacuug uagccguagc cauagcuugg      540

cuacuuggua gcucaacaag ucaaaaagua auauauuuag uaaugauauu acuaauagcc      600

ccagcauaca guauaaggug cauaggugua ucaaauagag auuuuguaga agguauguca      660

ggcggaacau ggguagaugu aguacuugaa cacggaggau gcguaacagu aauggcgcaa      720

gacaaaccca caguagacau ugaacuagua acaaccacag uaagcaauau ggcugaagua      780

agaucuuauu guuacgaagc aaguauaagc gauauggcaa gugauucaag auguccuaca      840

caaggcgaag cauauuugga uaagcaaagu gacacucaau auguuuguaa aagaacuuua      900

gucgacagag gauggggaaa uggaugcgga cuuuuuggaa aaggaagccu aguaacaugc      960

gccaaauuca cauguaguaa aaaaaugaca ggaaaaagua uacaaccuga aaaccuagag     1020

uacagaauaa ugcuuagugu acacggaagc caacacuccg gaaugauugu aaaugauauu     1080

ggucaugaaa cagacgaaaa uagagcaaaa guagaaguua ccccaaacuc accuagagca     1140

gaggcaacac uagguggauu uggcucauua ggauuagauu gugaacccag aacagggcua     1200

gacuuuagug acuuauauua ucuaacgaug aacaauaaac auugguuagu ccauaaagag     1260

ugguuccaug auauaccccu accauggcau gcgggagcag auacugguac accacauugg     1320

aacaacaaag aagcauuagu agaauuuaaa gacgcucaug cuaaaagaca aacuguagua     1380

guauuaggau cacaagaagg ugcagugcac acagcauuag caggugcucu agaagcagaa     1440

auggauggag caaaaggaag auuauucuca gggcaucuaa aaugccgauu aaaaauggau     1500

aaauuaagau uaaaaggggu aaguuauuca uuauguacag cagcauucac uuuuacuaaa     1560

guaccagcag aaacauuaca cgguacaguc acaguagaag uacaauaugc aggaacagau     1620

ggaccaugca aaguaccagc acagauggca guagauaugc aaacauuaac accaguugga     1680

cguuuaauaa cagcgaaucc aguaauaaca gaaucaacag aaaauaguaa aaugaugcua     1740

gaguuggauc cgccauuugg ggauucuuac auaguuauag gaguggguga caaaaaaaua     1800

acucaccauu ggcacagaag cgguucaaca auaggaaaag cauuugaagc aacaguaaga     1860

ggagccaaac gaauggcagu acucggagau acagcauggg auuuuggauc agucggaggu     1920

guauuuaacu cauuaggaaa agguauacau cagauauucg gggccgcuuu uaaaucauua     1980

uuuggcggaa ugucaugguu cagucaaaua uuaauuggca cuuuauuggu uuggcuagga     2040

cuuaauacaa aaaaugggag uauaagccuu accuguuuag cguuaggagg aguuaugaua     2100

uuuuugucaa ccgcaguaag ugcggaugua ggaugcagug uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9251
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 3 opt

<400>  9251
gggagaaagc uuaccaugag gauuauaaac gcaaggaaag agagaaaaag acguggagcc       60

gauacaagca ucggaauaau aggccuauua cuaacaacag ccauggcagc ugagauuaca      120

agaagaggaa gcgcauauua cauguauuua gauagaagug augcuggaaa agcaauauca      180

uuugcaacaa auuuaggagu aaauaaaugu cauguccaaa uaauggacuu agggcacaug      240

ugcgaugcaa caaugagcua ugaaugucca auguuagaug aaggagucga accagaugac      300

guagauuguu gguguaauac aacaucaaca uggguaguau acgguacuug ccaucauaag      360

aaaggagaag cgagaagauc aagacgagcc gugacucucc cgagucacag uacacguaaa      420

uuacaaacuc gaucacaaac augguuagaa ucucgagaau auacaaaaca uuuaaucaaa      480

guagaaaacu ggauauucag aaauccagga uucgcacuag ccgcaguagc aauagcaugg      540

uuauuaggga guaguacuuc ucaaaaagua aucuaucucg uaaugauccu auuaauugca      600

ccagcauaua gcauacguug cauaggagua agcaacaggg auuuugugga agguaugagu      660

ggaggaacau ggguagaugu aguauuagaa cacggagguu gcguaacugu aauggcccaa      720

gacaaaccaa caguagauau agaacucgua acaacaacag uaucaaauau ggcagaagua      780

agaaguuauu guuaugaagc caguauuuca gauauggcau cagacuccag augcccgaca      840

caaggggaag ccuauuuaga caagcaaagu gauacacaau auguauguaa acgaacauua      900

guugauaggg gaugggguaa uggaugugga uuauucggaa aaggaucauu aguaacaugu      960

gcaaaauuca cuuguuccaa gaaaaugacc ggaaaaucaa uacaacccga aaacuuagaa     1020

uacagaauaa ugcuaucugu acauggauca caacacagug gaaugauagu aaaugacgaa     1080

aauagggcaa aaguagaagu cacaccaaac agcccucgag cggaagcaac auuaggagga     1140

uuuggaaguc uaggauuaga cugugaacca aggacugguc uagauuuuuc ggaccuauau     1200

uauuuaacca ugaauaacaa acauugguua guacauaaag aaugguuuca ugauauucca     1260

uuaccguggc augcuggugc agauacagga acaccgcauu ggaacaauaa agaagcauua     1320

guagaauuua aagaugcaca ugcaaaaaga caaacaguag uaguuuuggg cagccaagaa     1380

ggugcaguuc auacugcacu ggcuggagcu cucgaagcag aaauggaugg agccaaagga     1440

aggcuauuuu caggacacuu gaaauguagg cuaaaaaugg auaaacuaag auuaaaaggc     1500

guaucauauu cucuauguac agcggccuuu accuuuacaa aaguuccagc agaaacucua     1560

cauggcacag uuaccguaga aguacaauau gcaggcacag auggaccaug caaaguacca     1620

gcacaaaugg caguagacau gcaaacauua acacccguag ggagauuaau aacagcaaau     1680

ccaguuauaa cugaaucaac ugaaaacagc aaaaugaugu uggaacuaga uccaccuuuc     1740

ggcgauaguu acauaguaau agguguagga gauaagaaaa uaacacauca cuggcaucgu     1800

aguggaagca caauaggaaa agcauuugaa gcaacaguaa gaggggcuaa aagaauggca     1860

guacuaggag auacagcuug ggacuucgga ucaguagggg guguauuuaa uagcuuagga     1920

aaaggcauac accaaauauu uggagcagca uuuaaaaguu uauuuggggg aaugaguugg     1980

uucucacaaa uauuaauagg aacuuuauug guuugguuag gucucaacac uaaaaacggu     2040

aguauaucau uaacaugucu agcacuagga ggaguaauga ucuuuuuauc aacagcagua     2100

ucagcagacg uagguuguag uguagauuuu ucaaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9252
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 3 opt

<400>  9252
gggagaaagc uuaccaugcg aauaauuaau gcaagaaaag aaagaaaacg uaggggagca       60

gacacaucaa uuggcauugu cggauuauua uuaacaacug cuauggcagc agaaaucaca      120

agaagaggua gugcuuauua uauguauuua gauaggagug augcaggaaa agcaauauca      180

uuugcaacca cacuaggcgu aaauaaaugu caugugcaga uaauggauuu aggacauaug      240

ugcgaugcua cuaugaguua ugaaugucca auguuagaug aaggaguaga acccgaugau      300

guagacuguu gguguaacac aacgaguaca ugggugguau auggaacaug ucaucauaag      360

aaaggagaag caagaaggag uagaagggcc guuacacuac cuagucauag uacaagaaaa      420

uuacaaacaa gaagccaaac augguuggaa agccgcgaau acacaaaaca uuuaauaaaa      480

guagaaaauu ggauuuucag aaauccgggc uuugcccuag uagcaguagc aauugcaugg      540

uuacuaggaa guuccacaag ucaaaaagua auuuauuuag uaaugauauu acuaauagcg      600

ccagcauacu cuauaaggug uauuggagua aguaauagag auuuuguaga aggcaugagu      660

ggaggaacuu ggguagaugu aguacuagaa cauggaggau gugucacagu gauggcccaa      720

gacaaaccca caguagauau agagcuagua acaacuaccg uaaguaacau ggcggaagua      780

agaucauacu guuaugaagc aaguauauca gacauggcuu ccgauaguag auguccgaca      840

caaggagaag cguaccugga uaaacaaagc gacacacaau auguauguaa aagaaccuua      900

guagauagag gauggggaaa ugguuguggu uuauuuggaa aggguagcuu aguaacaugu      960

gcaaaauuua cauguucaaa gaaaaugacg ggaaaaucaa uacaaccuga aaacuuggaa     1020

uauagaauaa ugcuaagugu acauggauca caacauagug gaaugauagu aaaugauaca     1080

ggacaugaaa cggaugaaaa ucgagcaaaa guggaaguaa cgccaaacuc accaagagcg     1140

gaagcgacau uaggagguuu cggaucauua ggguuggacu gugaaccacg aacaggacua     1200

gauuucagug auuuauacua uuuaacuaug aauaacaaac auugguuagu ucauaaagaa     1260

ugguuucaug auauaccauu accauggcac gcaggcgcag auacgggaac accacauugg     1320

aacaauaaag aagccuuagu cgaauuuaaa gaugcacacg cgaaacgcca aacuguagua     1380

guuuuaggau cacaagaagg agcaguacau acagcauuag cuggugcauu ggaagcggaa     1440

auggauggag caaaaggaag auuauuuagc ggucauuuaa aaugucgacu aaaaauggau     1500

aaauuaagau uaaagggagu aucauauuca cuauguacag cagcauuuac uuuuaccaaa     1560

guuccagcug aaacauuaca uggaacggua acuguagaag uacaauaugc aggaacugau     1620

ggaccuugua aaguaccugc ucagauggca guugauaugc aaacauuaac accuguagga     1680

cgauugauaa ccgcaaaucc agugauaaca gaaucuacag aaaauaguaa aaugauguug     1740

gaauuagauc caccuuuugg ugacaguuau auuguaauag gaguagguga uaagaaaauu     1800

acacaccacu ggcacaggag ugguaguacu auaggaaaag ccuuugaagc uacaguaaga     1860

ggagcaaaaa gaauggcugu guuaggagau acagcuuggg acuuugguuc aguuggcgga     1920

guuuuuaauu cguuagggaa aggcauucau caaauauucg gugcagcauu caaaagccua     1980

uucggaggaa ugagcugguu cagucaaaua cuuauaggaa cacuauuagu guggcuuggu     2040

uuaaauacga aaaauggauc aauaaguuua acauguuuag cauuaggugg uguaaugaua     2100

uucuuaagua cagcaguaag cgcagaugua ggauguucug uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9253
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 3 opt

<400>  9253
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag agcgaaaaag aagaggagca       60

gacacaucua uugggauagu aggacuauua uuaacaacag cgauggcggc ugaaauuaca      120

agaagaggaa gugcuuacua uauguacuua gaccgaagug augcuggcaa agcaauaucg      180

uucgcuacaa cacuuggggu aaacaaaugc cacguucaaa uaauggacuu aggacauaug      240

ugugaugcua cgaugagcua cgaaugucca auguuagaug aagguguaga accagaugac      300

guagacuguu gguguaacac aacaaguaca uggguaguuu auggaacaug ucaacacaag      360

aaaggggaag caagacguag cagacgagca gugacacuac cuagucauag uacuagaaaa      420

cuacaaacaa gaucacagac cugguuagaa aguagagaau auacaaaaca ucuaauaaaa      480

guagaaaauu ggauauuuag aaaucccgga uuugcccuag uugcaguagc aauagcaugg      540

uuauuaggau caagcacaag ucaaaaagua auauauuuag uaaugauauu acuuauagca      600

ccagcuuaca gcauuagaug uauaggugua ucaaauagag acuuugugga ggguaugagc      660

ggaggaacau ggguugaugu aguauuagaa cauggaggcu guguuacugu aauggcucaa      720

gacaaaccca caguagauau agaauuagua acgacaacag uaucgaauau ggcagaaguc      780

agaucuuauu guuacgaagc gagcauauca gacauggcaa gugauaguag augucccacu      840

caaggugaag cauauuuaga caaacaauca gauacacaau auguauguaa aaggacauua      900

guagaccggg guuggggaaa uggaugcgga cuauuuggua aaggcagcuu aguuacaugu      960

gccaaauuua cauguucuaa gaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa     1020

uauagaauua uguuaagugu acauggaagc caacauucag gaaugaucgu aaaugacauu     1080

ggacaugaga cugaugaaaa uagagcaaaa guagagguaa ccccaaauuc uccuagggca     1140

gaagcaacac ucggaggauu cggaaguuua ggacuagauu gugaaccaag aacaggacua     1200

gauuuuucag auuuauacua ucuaacaaug aauaauaaac auuggcuagu gcauaaagaa     1260

ugguuucacg acauuccgcu accauggcau gcaggagcag auaccggaac accacauugg     1320

aauaacaaag aagcucuggu agaauuuaaa gaugcacaug caaaaaggca aacgguagua     1380

guucuuggau cacaagaagg agcaguacac acggcauuag caggagcauu agaggcugaa     1440

auggauggug cuaaaggucg gcuauuuuca ggucauuuaa aaugcagguu aaaaauggau     1500

aaauugagau uaaaaggagu aagcuauagu cuauguacag cuguuugcac agcugcaaaa     1560

guaccagcag aaacauuaca uggaacugua acaguagaag uacaauacgc aggaacagau     1620

ggaccuugua aaguaccagc acaaauggcu guagauaugc aaacacuuac cccaguagga     1680

cgauuaauua cagcaaaccc cguaauaaca gagucaacgg aaaauaguaa aaugauguua     1740

gaauuagacc cucccuuugg ugauucauac auaguaauag gaguaggaga uaagaaaaua     1800

acacaccauu ggcacagauc aggcucaaca aucggaaaag ccuucgaagc uacaguacga     1860

ggagcaaaaa gaauggcagu auuaggagau acugcauggg auuuuggcag uguaggagga     1920

guauuuaaua guuuagguaa agguauucau caaauuuuug gagcagcuuu caaaucauua     1980

uucggaggaa ugaguugguu uucacaaaua cuuauaggaa cacuauuagu augguuagga     2040

cuuaauacga aaaacggguc aaucagcuua acauguuuag cacuaggugg cguaaugaua     2100

uuucuuucaa cagccguuag ugcagaugua ggcuguuccg uugauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9254
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 3 opt

<400>  9254
gggagaaagc uuaccaugag aauaauaaac gcuagaaaag aacggaagag aagaggagcu       60

gaugcgucaa uaggaauagu aaguuuauua uuaacaacag uaauggcugc agaaauuaca      120

aggagaggau cagcuuauua uauguauuua gacagaagug augcgggaaa agcaauaagu      180

uuuguaacaa cauuaggagu aaauaaaugu cauguccaaa uaauggacuu ggggcauaug      240

ugcgaugcaa caaugucuua ugagugccca auguuggaug aaggaguuga accagaugau      300

guagauuguu gguguaauac aacaucaacu uggguaguau acggaacaug ccaccacaag      360

aaaggggaag cuagaagauc aagaagggca guaacauugc caagucauag uacgagaaaa      420

cuacaaacac guagccagac auggcuugaa ucaagagaau auacaaaaca cuugauaaaa      480

guagagaauu ggauuuuccg aaauccaggu uucgccuuag uugcaguagc aauugcaugg      540

uuacuaggaa gcagcacuuc ucaaaaagua auauauuuag uaaugauauu acuaauagca      600

ccagcauauu caauaagaug uauaggagug aguaacagag auuucguaga agguaugagu      660

ggaggaacau ggguagacgu aguacucgaa cacgguggau gcguaacagu aauggcucaa      720

gacaaaccaa caguagauau ugaacucgua acaacaacag uaucaaauau ggccgaaguu      780

aggucuuauu gcuaugaggc aucaauauca gacauggcau cagauagcag auguccuaca      840

caaggagaag cauauuuaga uaaacaauca gacacacaau acguaugcaa aagaacauua      900

guagauagag gcuggggaaa cggcugugga cuguuuggga aaggaucucu cguaacaugc      960

gcuaaauuca ccuguaguaa gaaaaugaca ggaaaaagua uacaaccaga aaaccuagaa     1020

uauagaauua uguuaagcgu gcauggaagc caacacagcg ggaugauagu uaaugaugaa     1080

aauagggcaa aaguagaggu cacgccaaac agcccaagag cagaagcaac auuaggaggu     1140

uucggaagcc ucggauuaga uugugaacca aggacuggau uagauuucuc agauuuauau     1200

uaucuuacaa ugaauaacaa acacuggcua guacauaaag aaugguuuca cgauauucca     1260

uuaccauggc auucaggagc agacacugaa acaccacacu ggaauaauaa agaagcguug     1320

guagaauuua aagaugcaca cgcaaaaaga caaaccguag uagucuuagg uucgcaagaa     1380

ggagcaguac auacagcacu agcaggcgcg cuagaagcug aaauggaugg ugcaaaagga     1440

agacugucau caggucaucu aaaauguaga uuaaaaaugg auaaauuaag acuaaaaggu     1500

guaucauauu ccuuauguac cgcagcguuu acuuucacaa aaguuccagc cgaaacauua     1560

cauggaacag uaacuguaga aguacaauau gcagggcgag acggaccuug uaaaguucca     1620

gcacaaaugg caguugauau gcaaacauua acaccaguug ggagauuaau aaccgcaaac     1680

ccaguaauaa cugaaucaac cgaaaauuca aagaugaugu uagaauuaga ucccccauuu     1740

ggagacuccu auauagugau aggaguugga gauaagaaga uaacccauca uuggcauaga     1800

ucaggaagca uuauaggaaa agcauuugaa gcaacaguaa gaggcgcaaa aagaauggca     1860

guacuaggcg auacggcaug ggacuuugga ucagugggag gaguuuucaa cagucuagga     1920

aaagguauuc accaaauauu cggagccgca uucaaaagcc uauucggagg uaugucaugg     1980

uuuucacaga uucuaauagg aacgcuucua guaugguugg gauuaaauac uaaaaaugga     2040

aguauaucac uaacaugucu ugcuuuagga ggaguaauga uauucuuguc aacagcaguu     2100

ucugcagacg uuggaugcag uguggauuuu aguaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9255
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 3 opt

<400>  9255
gggagaaagc uuaccaugag aauaauaaau gcaagaaaag aaagaaaaag aagaggagcu       60

gauacaucua uuggaauagu aggcuuguua uuaacaacag cuauggcagc agaaauaaca      120

agaagaggaa gcgcuuacua uauguauuua gacaggagug augccggaaa agcaauauca      180

uuugcaacaa cauuaggggu caauaaaugu cauguccaaa uaauggacuu aggacauaug      240

ugcgacgcaa caaugaguua ugagugucca auguuggacg aaggaguaga accagaugau      300

guagauuguu gguguaauac gacuuccaca uggguaguuu acggaacuug ucaucauaag      360

aaaggagaag cuagaagaag caggagagca guaacacuac caucacauag uacuagaaaa      420

cuucaaacaa gaagccaaac auggcuagag ucaagagaau auacaaaaca cuugauaaaa      480

guagaaaauu ggauauuuag aaauccaggc uuugcauuag uggcaguagc uauagccugg      540

cuuuuaggaa gcaguacaag ucaaaaagua auauauuuag uaaugauauu auuaauagca      600

ccagcauauu caaucagaug caucggagug aguaauagag acuucguaga agguaugagc      660

ggaggaacau ggguagaugu gguacuagaa cacggaggcu guguaacagu aauggcacaa      720

gauaagccga ccguagauau ugaauuagua accaccaccg ugaguaauau ggcagaaguu      780

agaucauauu gcuaugaagc aaguaucagu gauauggcaa gcgauaguag auguccgaca      840

caaggagaag cauaccuaga uaaacaaagc gacacacaau acguauguaa aagaacacuc      900

guagauagag gauggggaaa cggaugugga uuauuuggga aaggaucccu aguaacaugu      960

gccaaauuua cuuguucgaa gaaaaugacu ggaaaaagua uacaaccuga aaacuuagaa     1020

uauagaauaa ugcuaucagu acaugguagc caacauagcg gaaugauagu aaaugaugaa     1080

aacagggcaa aaguagaagu aacgccuaau agcccaagag cagaagcuac acuaggugga     1140

uuuggaaguu uaggacuaga uugcgaacca cgaacggggu uagauuuuag ugacuuauau     1200

uauuuaacua ugaacaauaa acauugguua guacacaaag aaugguuuca cgacauacca     1260

uuacccuggc augcaggagc ggauacagga acaccacauu ggaauaacaa agaagcucua     1320

guggaauuua aagaugcaca ugcaaaaagg caaacaguug uaguacuggg aucacaagaa     1380

ggagcgguuc auacagcccu agcaggagca cuagaagccg aaauggaugg agcgaaagga     1440

agauuauucu caggacauuu aaaaugucga uuaaaaaugg acaaacuacg auuaaaagga     1500

guuaguuacu cauuauguac agcagccuuc acguuuacaa aaguaccagc agaaacacua     1560

cacggaacag uaacuguaga aguacaauac gcuggaacag auggaccuug uaaaguaccu     1620

gcccaaaugg cgguagauau gcaaacauua acaccugugg guagguuaau aacagcaaau     1680

ccaguaauaa cugaaucaac agaaaacagu aaaaugaugu uagaauuaga uccacccuuu     1740

ggugauagcu auauagucau aggaguaggu gacaagaaaa ucacacauca uuggcaccga     1800

aguggcucaa cgauagguaa agcauucgaa gcaacaguuc gaggagcuaa aagaauggca     1860

guacuaggag auacagccug ggauuuuggg aguguaggug gaguauucaa uagccuaggg     1920

aaaggaauac accaaauauu cggggcagca uucaaaaguc uauuuggugg uaugaguugg     1980

uuuucacaaa uauuaauagg cacuuuauua guauggcuag gguuaaauac uaaaaacgga     2040

agcauuagcu uaacaugccu agcgcuggga gguguaauga uauuucuaag uacagcagua     2100

agugcagaug uaggauguag cguugauuuu ucaaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9256
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 3 opt

<400>  9256
gggagaaagc uuaccaugag aauuauaaau gcaagaaaag aaagaaaaag aaggggagcu       60

gacacuucaa uaggaauaau aggauuacua cuuaccacag caauggcagc agaaauaaca      120

cgaagaggau cugcauauua cauguaucuc gauagaagcg acgcagguaa agccauaagu      180

uuugcaacca cauuaggugu aaauaagugu cauguacaaa ucauggacuu aggacauaug      240

ugugacgcaa ccaugagcua ugaaugcccu augcuagacg aaggaguaga acccgaugac      300

gucgacuguu ggugcaacac uacaagcaca ugggucguuu augguaccug ucaucauaag      360

aaaggagaaa cucgaagauc aagaagaagu guaucucuua gauaucauua cacaagaaaa      420

cuacagacaa gaucucaaac auggcuagaa ucaagagaau auaagaaaca ucuaauaaug      480

guugaaaauu ggauauucag gaacccagga uuugcaauag uuaguguugc aauaacaugg      540

cuuaugggaa guuuaacaag ccaaaaaguu auauacuuag uaaugauagu ucuaauugua      600

ccagcuuauu caauaagcug cauaggagug aguaacagag auuuagucga aggaauguca      660

gguggaacau ggguagaugu aguauuagaa caugggggau gcguuacaga aauggcccaa      720

gauaaaccga caguagauau cgaauuagua acaaugaccg uaucaaacau ggccgaagua      780

agaucauauu guuaugaagc aucacuauca gauauggcaa gugcaagcag augcccaacu      840

caaggggaac caucucuaga uaaacaauca gacacacaau caguaugcaa aagaacauug      900

ggugauagag gauggggcaa cggcuguggc auuuuuggaa aaggaagucu aguuaccugu      960

aguaaauuca cgugcuguaa gaaaaugcca ggaaaaagua uucaaccaga aaaccuugaa     1020

uacagaauua uguuaccagu acauggaucg caacauagug gcaugauagu gaaugauaua     1080

ggucacgaaa cagacgaaaa uagagcaaaa guugaaguga caccaaacag uccaagagca     1140

gaagcaacau uaggaggauu uggaucauua ggauuagauu gugaaccacg aaccgggcua     1200

gauuucagcg aucuuuauua cuuaacgaug aauaauaaac acuggcuagu acauaaagaa     1260

ugguuccaug acauaccauu accauggcac gcaggagcag auacuggaac uccacauugg     1320

aauaauaaag aagcacuugu ugaguucaaa gaugcacaug caaaaaggca aacaguagua     1380

guacuaggaa gccaagaagg agcuguucac acagcguuag caggcgcacu ugaagcugaa     1440

auggauggag caaaaggaag auuguuuuca ggacauuuaa agugcagacu uaaaauggac     1500

aaacuaagac uaaaaggcgu cucuuacuca uuauguacag cagcauuuac auucacuaaa     1560

gugccagcag aaacacuaca uggaacuguc acaguagaag uccaaucugc agguacagau     1620

ggcccaugua aaguacccgc ccaaauggca guugacaugc aaacgcugac accaguaggu     1680

agacuaauaa cagcaaaccc uguaaucaca gaaucaacag aaaacuccaa aaugauguua     1740

gaacuagauc caccuuucgg agauaguuau auuguaauug gaguaggaga uaagaaaaua     1800

acacaucauu ggcauagaag cggaaguacu auaggaaaag ccuucgaagc uacaguaaga     1860

ggcgcuaaaa ggauggcugu cuuaggugau acagcuuggg auuuuggauc aguagggggu     1920

guauucaaua gucuaggaaa aggaauacau caaauuuuug gagccgcguu caaaaguuua     1980

uucggaggua ugucgugguu uucacaaauc uuaauaggaa cauuacuggu auggcuagga     2040

cuaaauacua aaaauggcag uauuagcuua acauguuuag cuuuaggagg aguaaugaua     2100

uuucuuagca cagcaguuag ugcggauguc ggauguucug uagacuuuuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9257
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 3 opt

<400>  9257
gggagaaagc uuaccaugag aauuauuaac gcucguaaag aaagaaaaag acguggugca       60

gacacaagca uagggauaau agguuugcua uuaacaacug caauggcugc ugaaauaaca      120

agacgaggau cagcauauua uauguaccug gauaggucug augcaggaaa agcaauauca      180

uucgcaacua caaugggagu aaauaaaugu cauguacaaa uaauggauuu aggacacaug      240

ugugaugcua caaugaguua ugaauguccu auguuagacg aaggaguaga accugaugau      300

guugacuguu gguguaauac uacuagcaca uggguuguau augggaccug ccaucauaag      360

aaaggagaag caaggccauc aagacgagca gucacauuac cgagucauag uacucgaaaa      420

uuacaaacuc gaagucaaac gugguuagaa ucacgugaau acacaaaaca cuuaauaaaa      480

guagaaaauu ggauacuucg aaacccuggg uuuacgguag cagccuuagc aauggcaugg      540

cuucuaggaa auaguacaag ucaaagggua auauaccuag uaaugauuuu auuaauagca      600

cccgcauauu caauaagaug cauaggagua agcaauagag auuuuguaga agguauguca      660

ggaggaacau ggguagaugu uguacuagaa cacggcggau gcguaacagu uauggcucaa      720

gacaaaccaa caguagauau agaauuagua acuacaacag uaucaaauau ggcugaagua      780

aggaguuauu guuaugaggc aaguaucuca gauauggcaa gugacaguag gugcccaacc      840

caaggcgagg cuuaucuaga uaaacaaagu gauacucaau acguauguaa aaggacgcua      900

guagauagag gauggggaaa cgguugugga cuauuuggaa aaggaagccu aguaacaugu      960

gccaaauuua cauguucaaa gaaaaugaca ggaaaaagua uacaacccga aaaucuagaa     1020

uauagaauua ugcuaagugu acauggaucu caacacucag gaaugauagu aaaugacaua     1080

ggacaugaaa cagaugaaaa uagagcaaaa guggaaguua caccaaauag uccaagagca     1140

gaagcuacac uggguggauu cgggagcuua ggguuagauu gugaaccaag gacaggauua     1200

gauuucucag aucuauacua ucuaacaaug aacaacaaac auugguuagu ucauaaagaa     1260

ugguuucacg acauaccacu accauggcac gcaggagcag auacaggaac accacauugg     1320

aacaauaaag aagcauuagu agaguucaaa gacgcacaug cuaaaagaca gacaguaguu     1380

guucuaggaa gccaagaagg agcuguacau acagcacuug caggagcacu ugaagcagaa     1440

auggauggug caaaagggag acuuuucagc ggacacuuaa aaugccgaau aaaaauggau     1500

aaauuaagau uaaaaggugu cucguauucu uuauguacag cagcauuuac auuuaguaaa     1560

guaccagcag aaacauuaca ugguacggua acagucgaag uacaauaugc aggaagagau     1620

ggaccaugua aaguaccagc acagauggca guagauaugc aaacauuaac accuguaggg     1680

agauuaauaa cggccaaucc uauaauaaca gaaaguacag aaaauucacg caugaugcuu     1740

gaacucgauc caccauucgg agauaguuau auagugauag gaguagguga uaagaaaaua     1800

acccaucauu ggcauagaag uggaaguacu auagguaaag cauucgaagc aacagugaga     1860

ggagcuaaac gaauggcagu auuaggcgau acagcuuggg auuuugguag uguaggugga     1920

guauucaaua gucuaggaaa aggaguacac caaguauucg gagcggcauu uaagagcuug     1980

uucggaggaa ugagcugguu cucacaaaua cuuauaggua cauuauuagu auggcuagga     2040

uuaaauacua aaaacggaag uauuucauua gcaugucuug cucuaggugg ugcaaugauu     2100

uuucuaagca cugccguuuc ugcagacguu gguuguagcg uagauuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  9258
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 3 opt

<400>  9258
gggagaaagc uuaccaugac uagcguagga auaguaggau uauuauuaac gacagcaaug       60

gcugcagaag uaacucgacg cggaucagca uauuauaugu accucgacag aaaugacgca      120

ggagaagcaa uuaguuuccc uacaacauua gggaugaaua aauguuauau ccaaaucaug      180

gauuuaggac acauguguga ugcaacaaug agcuacgaau guccaauguu agacgaaggu      240

guagaaccag acgacguaga cuguuggugc aauacaacau caacaugggu aguauauggu      300

acaugccauc auaagaaagg cgaagcuaga agaaguagac gagcaguaac acuaccauca      360

cauagcacaa gaaaauuaca aacaagaucu caaaccuggc uagaaucaag agaauauacu      420

aaacauuuga uaagaguaga aaauuggaua uuuagaaauc caggauucgc uuuagcagca      480

gcugccauug ccuggcuuuu aggaucauca acaagccaaa aaguaauaua uuuaguaaug      540

auauugcuua uagcgccagc auacagcaua agauguauag gaguuucaaa uagagauuuu      600

gucgaaggaa ugagcggagg aacaugggua gauauaguac uugaacaugg aggaugcgug      660

acaguuaugg cccaagacaa accuaccgua gacauagaau uaguuacaac uacuguaucu      720

aauauggccg aaguaagaag uuauugcuac gaggcuucaa uuagugacau ggcaucagau      780

ucuagaugcc caacucaagg ugaagcauac cuagacaaac aaagugacac gcaauacguc      840

uguaaaagaa cauuaguaga cagaggaugg ggcaauggau gcggauuauu cggaaaaggc      900

agcuuaguaa cuugugcaaa auucgcaugu agcaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaacu uagaauacag aaucauguug ucaguucaug gcagccaaca uucaggaaug     1020

auaguaaacg auacuggaca ugaaaccgau gaaaauagag caaaaguaga aauaacucca     1080

aauaguccua gagccgaagc cacauuaggu gguuuuggau cauuaggacu agauugugaa     1140

ccacgaacag gacuagauuu uagugauuua uauuaucuaa cgaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ucaugauaua ccacuuccau ggcaugcagg agccgauacu     1260

ggaaccccac acuggaauaa uaaagaagca uuaguugagu ucaaagaugc ccacgcaaag     1320

agacaaacag uaguaguauu aggaucacaa gaaggggcag uacauaccgc cuuagcugga     1380

gccuuagaag cagaaaugga cggagcuaaa ggaagacuga guagcggaca cuugaaaugu     1440

cgacugaaaa uggauaaguu aagguuaaaa ggaguaaguu auaguuuaug uacagcugca     1500

uuuacauuua ccaaaauacc agccgaaacc cugcauggga cgguuacagu agaaguacaa     1560

uaugcaggaa cggauggacc auguaaagua ccagcgcaga uggcaguuga uaugcaaaca     1620

cuaacaccag uaggaagacu uauaaccgca aauccgguga uaacagaaag uacagaaaac     1680

uccaaaauga uguuagaauu agauccacca uuuggagacu cauauauagu gauaggagug     1740

ggagaaaaga aaauaacaca ccauuggcau agaaguggaa guacaauagg aaaagcguuc     1800

gaagcaaccg uaagaggagc aaaaagaaug gcaguucuag gagauacugc augggauuuc     1860

gguagcguag gaggagcauu aaauucacua ggaaaaggaa uccaucaaau auuuggagca     1920

gcauucaaaa guuuauucgg aggaaugagu ugguuuuccc aaauacuaau aggaacacua     1980

uuaauguggu uaggauugaa uacgaaaaau ggaucuauau cgcugaugug ucuagcauua     2040

ggaggagucu uaauuuucuu aucaacagca guaucagcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9259
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 3 opt

<400>  9259
gggagaaagc uuaccaugac aucaguugga auagucggac uucuuuugac aacagcaaug       60

gcugcugaag uuacgcgaag aggaagugcc uauuacaugu auuuggacag aaaugaugca      120

ggagaggcua uuucauuccc aacaacauua gggaugaaca aauguuauau acaaauaaug      180

gaucuugguc auacaugcga cgcgacgaug ucauacgaau guccaaugcu ugaugaagga      240

guagaaccug acgauguaga cuguuggugc aacacaacau caacuugggu aguauaugga      300

acuugucauc acaaaaaagg ggaggcaagg agaucacgua gagcaguuac acuaccuuca      360

cauaguacaa gaaaacuaca aacaagauca caaacauggc ucgaaucacg agaauauaca      420

aaacauuuaa uaaggguaga aaauuggauu uucagaaacc caggauuugc ccuugcagca      480

gcagcaauug cauggcuuuu aggaaguagc acaagucaga aagugauaua cuuagugaug      540

auuuuacuua uagcuccagc auauucuaua agaugcauag gaguaaguaa uagagacuuc      600

guagaaggaa ugaguggggg uacuugggua gauguuguac uugaacacgg cggaugcgua      660

acaguaaugg cccaagauaa accaacugua gauauagaau uaguaacgac aacaguuuca      720

aauauggcag aaguaagauc auauugcuac gaagcaucca uaucagacau ggcuucugau      780

ucuagauguc caacucaagg ugaagccuau uuagauaaac aaucagauac ucaauaugua      840

uguaaaagaa ccuuaguaga cagagguugg ggaaauggau gcggauuauu uggaaaagga      900

agucuaguaa cgugugcaaa auuugcaugc aguaaaaaaa ugacuggaaa aaguauucaa      960

ccagaaaacc uugaauauag aauaauguug aguguacaug guagccaaca uucaggaaug     1020

auaguaaaug auacaggcca cgaaacagac gaaaauagag caaaaguuga aauaacacca     1080

aauaguccca gagccgaagc aaccuuagga ggauucggau cucuaggauu agacugcgaa     1140

ccuagaacag gacuagacuu uucagaucuu uacuauuuaa caaugaauaa uaaacauugg     1200

cuagugcaua aggaaugguu ucacgauaua ccauuaccau ggcaugcagg agcugauaca     1260

gguacaccac auuggaauaa uaaagaagca cuuguagaau ucaaagacgc acacgcuaaa     1320

cgacaaacag uaguaguauu agguagucaa gaaggagcag uucauacagc auuagcuggg     1380

gcauuagaag cagaaaugga uggagcaaag ggaaggcuau caucaggaca uuuaaaaugc     1440

agauuaaaaa uggauaaauu gagacucaaa gguguaucau auucucuaug uacagcagcu     1500

uucacuuuua caaaaauacc agcagaaacu cuucauggga cuguuacagu ugaaguacaa     1560

uaugcaggaa cagauggucc augcaaagua ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccag uaggaagacu gauaacagca aacccaguaa uaacagaaag cacagaaaau     1680

ucaaaaauga uguuagaauu agauccucca uucggagauu cauauauagu cauaggagua     1740

ggagaaaaaa aaauaacaca ucauuggcau agaucaggau caacuauagg caaagcauuu     1800

gaagcaacag uaagaggcgc aaaaagaaug gccguacuag gagacacagc uugggacuuc     1860

ggaagcguag gaggagcacu aaauaguuua ggaaaaggaa uucaccaaau auuuggagca     1920

gcauuuaaaa guuuauucgg aggaaugagc ugguucucac aaauauuaau agguacuuua     1980

cuaauguggu uaggacuaaa ugccaaaaac ggaucaauaa gcuuaaugug cuuagcucuu     2040

ggaggugugc uaauauuccu uucaacagca guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9260
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 3 opt

<400>  9260
gggagaaagc uuaccaugac aucaauugga auaauugguc uuuuacuuac uacagcaaug       60

gcagcagaaa uaacaagaag aggaucagca uauuauaugu auuuagaccg uagcgaugcc      120

ggaaaagcaa uaucauucgc uacaacauua ggaguuaaua aaugucaugu ucaaauaaug      180

gacuuaggac auauguguga ugcaacuaug ucuuaugaau guccaaugcu agacgaagga      240

guagaaccag augauguaga uugcuggugu aauacuacua gcacaugggu uguauauggu      300

acaugucauc auaagaaagg cgaagcuaga agaaguagac gagcaguaac uuuaccaucu      360

cauucgacaa gaaaauugca gacgagauca caaacauggu uagaaaguag agaauauacu      420

aaacauuuaa uuaaaguaga aaacuggaua uuuagaaauc cggguuuugc auuaguggca      480

gucgcuauug caugguuauu aggcucauca acgagucaaa aaguaauaua ucuaguaaug      540

auacuauuga uagccccagc cuauucaaua agaugcauag gaguaaguaa uagagauuuu      600

guagaaggua ugagcggagg cacuugggua gaugucguuu uagaacacgg aggaugcgua      660

acaguaaugg cucaagacaa accaaccgua gauauagagu uaguuacaac aacaguaagu      720

aauauggcag aaguucgauc uuacuguuau gaagcaucaa uaucagauau ggcuagcgac      780

ucuagaugcc ccacacaggg agaagccuac uuagauaaac aaagugauac ccaauacgua      840

uguaaaagaa cccuaguaga uagaggaugg ggcaaugggu gcggacuauu ugguaaagga      900

ucucuaguga caugcgcaaa auucacaugc ucaaagaaaa ugacaggaaa aaguauccaa      960

ccagaaaauu uagaauauag aauaauguua ucuguacaug gcucacaaca uucagguaug     1020

auaggauaug aaacugauga agacagagca aaaguagaag ucacaccaaa cuccccaaga     1080

gcagaagcaa cacuuggcgg guuuggaagu uuaggauuag acugugaacc uagaacagga     1140

uuagacuuca gugauuuaua uuaccuaacg augaacaaua aacacugguu aguacauaaa     1200

gaaugguuuc acgacauacc auuaccgugg caugcaggag cagauacugg aacaccucau     1260

uggaacaaua aagaagcauu ggucgaguuc aaagaugcuc augcaaaaag acaaaccgua     1320

guaguauuag gaucacaaga aggagcugua cauacagcau uagcaggagc gcuagaagca     1380

gaaauggaug gagcaaaagg aaggcuauuc ucaggccauc uaaaauguag gcuuaaaaug     1440

gauaaacuac gacugaaagg aguaaguuac ucauuaugua cagcagccuu caccuucacg     1500

aaaguaccag ccgaaacucu acacggaaca guaacuguag aaguacaaua ugcagguaca     1560

gauggacccu guaaaauacc aguacaaaug gcaguagaua ugcaaacauu aacaccagua     1620

ggaagauuaa uuacugcaaa uccaguaaua acagaaagua cagaaaauuc aaaaaugaug     1680

cuugaacuug aucccccauu uggagauuca uauauaguaa uaggaguagg agauaagaaa     1740

auaacacauc auuggcauag aucaggaucg acaauuggaa aagcauucga agcaacagua     1800

agaggagcga aaagaauggc uguauuagga gacacagcgu gggauuuugg aucaguggga     1860

ggugucuuua auagcuuagg aaagggaaua caccaaauau ucggugccgc guuuaaauca     1920

cucuuuggag gcaugucaug guucagucaa auacuaauag gaacucuguu aguguggcua     1980

ggauuaaaua cgaaaaaugg aagcauuagc uuaacauguu uagcauuagg aggagugaug     2040

auauuccuaa guacagcagu aucagcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9261
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 3 opt

<400>  9261
gggagaaagc uuaccaugac aucaguagga auuguuggau uauuacuaac aacagcuaug       60

gcagcugagg ucacaagaag aggaucugca uauuauaugu auuuagauag gaaugaugca      120

ggagaagcua uaaguuuccc gacaacccua gguaugaaua aauguuauau ucaaauaaug      180

gaucuaggac acauguguga ugcuacaaug ucauacgaau gcccuauguu agaugaaggc      240

guagaaccag acgauguaga uugcuggugu aauacaacga gcacaugggu aguauaugga      300

acuugccauc auaagaaagg agaagcuaga agaaguagga gagcuguaac auuaccuagu      360

cauaguacaa gaaaauuaca aacaagaucg caaacauggu uggaaucaag agaauauacg      420

aaacauuuaa uaagaguaga aaauuggaua uuuagaaauc caggguucgc uuuagcagcg      480

gcugcuauug caugguuauu agggaguagc acuucacaaa aaguaauaua uuuagugaug      540

auauuauuaa uagcuccagc auauucaaua agaugcauag gagugucaaa uagagauuuc      600

guagaaggaa ugucgggugg aacaugggua gauguaguuu uggaacaugg cgguugcgua      660

acaguaaugg cucaagacaa accuacagua gauauagaau uaguaacaac aacaguaagu      720

aauauggcag aaguuagauc uuauugcuau gaagcaucaa uuucagauau ggcaagugac      780

ucacgaugcc ccacacaagg ggaagcauac cuagacaaac aaucagauac ucaauacgua      840

uguaaaagaa cauuaguaga ccgggguugg ggaaacggau gcggucuauu cggaaaagga      900

agucuaguaa caugugcuaa auuugcaugu aguaagaaaa ugacagggaa aaguauucag      960

ccugaaaacu uagaauauag aauaaugcug ucuguacaug gaagucaaca uagugguaug     1020

auaguaaaug auacaggaca ugaaacagac gaaaauaggg caaaagugga gaucacgcca     1080

aacaguccua gagcagaagc uacacuagga gguuuuggaa gucuagggcu agauugugaa     1140

cccagaacug gauuagauuu uucggauuua uacuacuuaa caaugaacaa caaacacugg     1200

uugguccaua aagaaugguu ucaugauaua ccauuaccau ggcaugcggg cgcagauaca     1260

ggaacaccac auuggaauaa caaagaagca uuagucgaau uuaaagaugc ccaugcaaaa     1320

agacaaaccg ugguagucuu aggaagucaa gaaggagcag uacauacagc acuagcuggc     1380

gcgcuugaag cagaaaugga uggagcuaaa ggaaggcuau cgucaggaca uuuaaaaugu     1440

agguugaaaa uggauaaacu aaggcuaaaa ggaguuaguu auagcuugug uacugcagca     1500

uucacguuua caaaaauacc agcagaaaca uuacacggua caguuacagu ugaaguacaa     1560

uaugcaggga ccgauggacc auguaaagua cccgcacaaa uggcuguaga uaugcagaca     1620

cuaacacccg uaggaagacu aauaacagca aauccuguca uaacagaaag uacagaaaac     1680

ucaaaaauga ugcuugaauu agauccacca uuuggagauu cuuacauagu aauaggagua     1740

ggagaaaaga aaauaacuca ucacuggcau cgaaguggaa guacaauagg aaaagcauuu     1800

gaagcaaccg uuagaggagc aaaaagaaug gcaguauuag gagacacagc uugggauuuu     1860

ggaagcguag gaggagcauu aaacucauua gggaaaggua uacaucaaau uuucggagca     1920

gcauuuaaaa gucuauuugg gggaauguca ugguuuaguc aaauauuaau agguacauug     1980

cuaauguggu uaggacuaaa uacuaaaaau ggaaguauaa guuuaaugug uuuagcacuu     2040

ggaggaguac uaauauuuuu aagcacagca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9262
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 3 opt

<400>  9262
gggagaaagc uuaccaugac cucaaucggu auuauaggac uacuauuaac aacagcaaug       60

gcggccgaaa uaacgagaag aggaucagca uauuacaugu aucuagaccg aagugaugca      120

ggaaaagcaa uaucuuuugc uacaacacua ggaguaaaca aaugucaugu acaaauuaug      180

gacuuaggac auauguguga cgcgacaaug ucauacgaau gucccauguu agaugaagga      240

guugaaccag augauguaga cuguuggugu aacacaacca guacuugggu aguauacggu      300

acaugccauc acaagaaagg agaagcaaga agaaguagac gagcagugac auuaccaagc      360

cacucaacga gaaaauuaca aacaagaagu caaacauggc uagaaaguag agaauacacu      420

aaacaucuaa uaaaaguaga aaauuggauc uucagaaauc caggauuugc auuaguagca      480

guugcaauug caugguuauu aggaucauca accagccaaa aaguuauuua uuuaguaaug      540

auccuauuaa uugcaccagc auacaguauu agaugcaucg gcguuucaaa uagagauuuu      600

guugaaggua ugucaggagg aacaugggua gauguaguau uagaacaugg uggaugugua      660

acuguuaugg cacaagauaa accuacagug gacauugaau uaguaacaac aacaguaucc      720

aacauggcag aaguuagauc auacugcuau gaagcaagca uaagugacau ggcuucagau      780

ucaagauguc cuacacaagg agaagcauau cuggacaaac aaucagauac ucaauauguc      840

ugcaaaagaa cauuaguuga uagaggcugg ggaaaugggu gcggcuuauu cggaaaaggu      900

agucuaguca caugcgcuaa auuuacaugu aguaagaaaa ugacaggaaa aucaauccag      960

ccagaaaacu uagaauacag aauuauguug ucaguacaug gaucucaaca uagugguaug     1020

auuguaaacg auacaggaua cgaaacagau gaaaauagag ccaaaguaga aguuaccccu     1080

aacucaccaa gagcagaagc aacucuagga ggauuuggau ccuuaggacu agauugugaa     1140

ccaagaacag gcuuagauuu uucagaucua uauuaccuua caaugaacaa uaaacacugg     1200

uuaguacaua aagaaugguu ccacgauaua ccacuaccau ggcacgcagg agcggacacc     1260

gguacaccac acuggaauaa uaaagaagca uuaguugagu ucaaagaugc ucaugccaaa     1320

agacaaacag uaguaguuuu aggaucucaa gaaggagcag uacauacagc uuuagcagga     1380

gcauuagaag cagaaaugga cggagcaaaa ggaaaauuau uuucaggaca uuuaaaaugc     1440

agauuaaaaa uggacaaauu gagauuaaaa ggcguaaguu auucacuaug uacggcagca     1500

uucacauuua cuaaaguccc agccgaaaca cugcauggaa caguuacagu agaaguucag     1560

uacgcaggca cagauggacc augcaaaaua ccuguacaaa uggcaguaga uaugcaaacc     1620

cuaacaccag ucggaagacu uauaacugcc aauccaguua uaacagaauc aacugaaaac     1680

uccaaaauga uguuagaauu agauccacca uuuggagaca guuauauagu aauuggagua     1740

ggagacaaga aaauuacaca ucacuggcau cgaaguggaa guacaauagg aaaagcauuu     1800

gaagcuacag uaagaggagc uaaaagaaug gcaguacuag gugauacagc augggauuuu     1860

gguucuguag gagguguauu uaauagucuu ggaaaaggaa uacaucaaau auuuggagcc     1920

gcauuuaaau cauuauuugg aggaauguca ugguucucac aaauacuaau uggcacauua     1980

uuaguauggc uaggacuaaa caccaaaaac ggaagcauaa guuuaacaug ucuagcacua     2040

ggaggaguaa ugauauuucu aaguacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9263
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 3 opt

<400>  9263
gggagaaagc uuaccaugac aucaauagga auuauugguc uacuucuaac aacagcaaug       60

gcugcagaaa uaacaagaag aggaagugca uauuacaugu auuuagacag aucagacgcu      120

ggaaaagcga ucaguuucgc aacaacuuua ggaguaaaua aaugucaugu acaaauaaug      180

gauuuagguc acauguguga cgcaacuaug uccuaugaau guccaauguu agacgaagga      240

guugaaccag augauguuga uuguuggugu aauacaacau cuacaugggu cguauaugga      300

acaugucaca ggaaaaaagg ggaagcaaga agaucuagaa gggcaguaac cuuaccaagu      360

cauucaacaa gaaagcuaca aacaagaagc caaacauggc uagaaaguag agaauacacu      420

aaacacuuaa uuaaaguaga aaauuggaua uuuagaaauc caggauucgc auuaguugca      480

guagcaauag caugguuauu aggcucguca acaagucaaa aaguuaucua uuuaguaaug      540

aucuuacuaa uagcuccagc cuauucaauu aggugcaucg gcguaucaaa uagagacuuu      600

guagaaggaa ugagcggagg uacaugggua gacguaguau uagaacaugg aggaugcgua      660

acaguuaugg cucaagauaa accaaccgua gauauagaac uaguaacaac aacaguuagu      720

aauauggcag aaguuagaag cuauugcuau gaagcgucua uaucagauau ggcaucagau      780

aguagguguc caacacaggg agaagcauac uuagacaaac aaucggauac ucaauaugua      840

uguaaaagaa cauuaguaga uagaggcugg gguaacggau gcggauuauu cggaaaagga      900

ucauuaguga ccugugcuaa auuuaccugc ucaaaaaaaa ugacgggcaa aucaauacaa      960

ccugaaaauc uagaauaccg cauaaugcuu aguguacaug gaucccagca uucaggaaug     1020

auaguaaacg auacaggcua cgaaacagau gaaaauagag caaagguaga aguuacaccu     1080

aauucaccac gagcagaagc aacauuagga ggauuuggaa gucuagguuu agauugugaa     1140

ccaagaacag gauuagauuu uucagaucua uauuauuuaa caaugaacaa uaaacauugg     1200

cuaguacaua aagaaugguu ucaugauaua ccauuaccuu ggcaugcagg ugcagauaca     1260

ggaacaccuc auuggaauaa uaaagaagcc cuugucgagu ucaaagaugc acacgccaaa     1320

agacaaacug uagucguacu aggaagucaa gaaggugcag uacacacagc uuuagcagga     1380

gcacucgaag cagaaaugga cggagcgaaa ggaaaacugu ucagcggaca uuuaaaaugc     1440

cgauuaaaaa uggacaaauu gagacuaaaa ggaguaucau acucuuuaug uacagcagca     1500

uuuacauuua caaaaguucc agcagaaaca uuacauggaa caguaaccgu agaaguacaa     1560

uaugcaggaa cugacggucc augcaaaaua ccaguucaaa uggcaguaga caugcaaaca     1620

uuaacuccag uggguaggcu aauuacagca aacccuguaa uaacagaaag cacggaaaac     1680

ucuaaaauga uguuagaacu agauccacca uuuggagauu cauacauagu aauuggagua     1740

ggagauaaaa aaauuacaca ucauuggcau agaaguggau caacaauagg aaaagcauuc     1800

gaagcaacug uacgaggggc aaaaagaaug gcaguacuag gcgacacagc augggauuuu     1860

ggaaguguug gggguguauu uaauucuuua ggaaaaggaa uacaucaaau auuuggagcg     1920

gcauucaaau cauuauuugg aggaaugagc ugguuuaguc aaauuuuaau agguacuuug     1980

cuaguauggu uaggauuaaa uacaaaaaac ggaucaauaa gccuaaccug uuuggcuuua     2040

ggaggaguaa ugauauucuu aagcacagca gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9264
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 3 opt

<400>  9264
gggagaaagc uuaccaugac cagcauagga auaaucggac uguuauuaac gacagcuaug       60

gcagcugaaa uaacaagaag gggauccgcg uacuacaugu acuuagauag aucugaugcu      120

ggaaaagcaa uaucauucgc uacaacacuc ggaguaaaca aaugucaugu acaaauaaug      180

gaucuaggac acauguguga ugccacaaug uccuacgaau guccuauguu agaugaagga      240

guugagccag augauguaga uugcuggugu aauacaacuu ccacaugggu aguauauggc      300

acaugccauc acaagaaagg agaagcuaga agaaguagga gagccguaac acuaccaagc      360

cauaguacaa ggaaauuaca aacaagaagu cagacuuggc uagaaaguag agaauauaca      420

aaacaucuga uaaaaguaga aaauuggaua uucagaaauc caggauuugc ucuaguagca      480

guagcaauag caugguuauu aggaagcuca acaagccaaa aaguuaucua uuuaguaaug      540

auauuauuaa uagcuccagc guauuccaua agaugcauag gaguaaguaa uagagacuuu      600

guagaaggaa ugaguggugg gacaugggua gauguugucu uagaacaugg gggaugugua      660

acagugaugg cucaagacaa accaacaguu gacauagagc uaguaacuac aacaguaagu      720

aacauggcag aaguaagauc auacuguuau gaagccucca uaucagauau ggcauccgac      780

agucgauguc caacacaagg ugaagcauau uuggacaaac aaagugauac acaguaugua      840

ugcaagagaa cauuaguuga uagaggaugg gguaacgggu guggauuauu uggaaaaggu      900

ucacugguga caugugcaaa auuuacaugc ucaaagaaaa ugacuggaaa aagcauacaa      960

ccagaaaauu uagaauauag aauuauguua ucaguucacg gaagccaaca uaguggaaug     1020

acuguaaaug acauaggaua ugaaacagac gaaaacagag caaaaguuga aguaacgcca     1080

aauucaccua gagcagaagc uacauuagga ggauuuggca guuugggauu agauugugaa     1140

cccagaacag gacuugacuu cagugauuua uauuaucuga caaugaacaa uaaacauugg     1200

uuaguacaua aagagugguu ucaugauaua ccauuaccgu ggcaugcagg agcagacaca     1260

ggaacuccac acuggaacaa uaaagaagcu cuaguagaau uuaaagacgc acacgcuaaa     1320

agacaaacag uaguagucuu agggucacaa gaaggcgcag uacacacagc uuuagcagga     1380

gcauuagaag cagaaaugga cggcgccaaa ggcaaacuau uuucuggcca uuuaaagugu     1440

aggcuaaaaa uggacaagcu aagauuaaaa gggguaucau auucauuaug cacagccgcc     1500

uuuacauuua caaagguucc agcagaaaca cugcauggaa cagugacagu agaaguccaa     1560

uaugcaggaa cagauggacc auguaaaaua ccaguacaaa uggccguaga caugcaaaca     1620

uuaacaccag uaggaagacu cauaacagca aacccuguca uuacagaaag cacagaaaau     1680

ucuaaaauga ugcuagaauu agauccaccu uuuggagauu cuuauaucgu gauaggaguu     1740

ggagauaaga aaauaacaca ucauuggcau agauccggaa guacaauagg aaaagcguuu     1800

gaagccacag uaaggggagc aaaaaggaug gcaguacucg gagauacagc gugggauuuu     1860

gggucaguug gaggaguuuu uaauagucua ggaaaaggua uacaccaaau auuuggggca     1920

gcauucaaau cuuuauucgg aggaaugagc ugguuuucac aaauauuaau aggcacacua     1980

cuuguauggu uaggauuaaa uaccaaaaau ggaucaauaa guuugacaug ccuagcgcua     2040

ggagggguaa ugauauuucu gucaacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9265
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 3 opt

<400>  9265
gggagaaagc uuaccaugac aaguauaggu auaguaggcc uucuacuaac gacagcaaug       60

gcagcugaaa uaacaagaag agguucagca uauuauaugu aucuagauag aagugacgca      120

ggaaaagcua uuagcuucgc aacuacuuua ggaguuaaua aaugucaugu acaaauaaug      180

gacuuaggac auaugugcga ugcaacaaug aguuaugaau gcccaauguu agaugaagga      240

guagaaccag acgauguaga uugcuggugc aauacuacau cgacaugggu aguguacggc      300

acaugucauc acaagaaagg agaagcgaga agaucaagaa gggcaguaac acuaccaagc      360

cauaguacga gaaaauuaca aacacgaucu caaacauggu uagaaucaag agaauauaca      420

aaacacuuaa uaaaagucga gaacuggaua uuucggaauc caggauucac ccucguugca      480

guagccauag cuuggcuacu aggauccuca acuagccaaa aaguaauuua uuuaguaaug      540

auauuauuaa uagcuccagc auauucaaua agauguauag guguaucuaa cagagauuuu      600

gucgaaggca ugucaggagg aacaugggua gauguaguuc uagaacaugg aggaugcgua      660

acaguuaugg cacaagacaa acccacugua gauauagaau uaguaacgac aacaguauca      720

aauauggcag aaguacgaag cuauugcuau gaagccucaa uaucagacau ggcuucagau      780

ucaagauguc caacacaggg ugaagcauau cucgacaaac aaucggacac acaauaugua      840

uguaaacgca cauuaguaga uagaggaugg gguaauggau gcggucuauu cggaaaagga      900

ucauuaguaa caugugcaaa auucacuugc ucuaagaaaa ugacgggaaa aucaauucag      960

ccagagaauu uagaauaccg uauaauguua ucuguucaug gaucacaaca uucugggaug     1020

auaguaaaug augaaaauag agcaaaagua gaagugacac caaauucacc aagagcagaa     1080

gcuacacuag gaggauuugg aucacuagga uuggauugcg aaccacgaac uggcuuagau     1140

uuuucagauc uauacuauuu aacaaugaau aacaaacauu ggcuaguaca uaaagaaugg     1200

uuccacgaua uaccauuacc auggcaugcu ggggcagaua cuggaacacc acauuggaau     1260

aauaaggaag cauuaguaga auuuaaagau gcucacgcaa aaagacaaac aguagucgug     1320

uuaggaaguc aagaaggagc aguacauacc gcauuagcug gugcacuaga agcagaaaug     1380

gauggagcaa aaggaagauu auuuucugga caucuaaaau gccgauuaaa gauggauaaa     1440

cuuagauuaa aagguguuag cuacagucua uguacagcag cauucacauu caccaaagua     1500

ccugcagaga cccuacacgg aacaguuacc guagaaguac aauaugcagg gacugaugga     1560

ccaugcaaag uaccugcaca aauggcagua gacaugcaaa cauuaacccc aguaggaaga     1620

uuaauaacag cuaauccagu aauaacagaa ucaacagaaa acagcaaaau gaugcuagaa     1680

uuggacccac cuuuuggaga uaguuacaua guaauaggag uaggagauaa gaaaauaaca     1740

caucacuggc auaggagugg uaguacaauu ggaaaagcau uugaagcaac aguuagagga     1800

gcuaaaagaa uggcagucuu aggagauaca gcaugggauu uuggaagugu aggagguguu     1860

uuuaauucgu uaggaaaagg aauacaucaa auauuuggag ccgcuuucaa aagucuuuuu     1920

ggaggaaugu caugguuuuc acaaauucuu auagggacau uauuaguuug gcuuggauua     1980

aacacuaaaa acggaaguau aucuuuaaca ugccuagcac uaggaggggu aaugauuuuu     2040

cuaaguacag caguaagugc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  9266
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 3 opt

<400>  9266
gggagaaagc uuaccaugac gucaauuggu auaauaggcc uauuacuaac aacagcuaug       60

gcggcagaaa uuacaagaag gggaucagca uauuauaugu accuagauag aucagaugca      120

gguaaagcua uaaguuuugc uacuacauug ggaguaaaua aaugucacgu acaaauaaug      180

gaucuggguc auaugugcga cgcuacaaug ucguaugaau guccaaugcu agacgaaggg      240

gucgaaccug augauguaga uuguuggugu aauacuacau cgacaugggu aguauauggc      300

accugucauc acaaaaaagg agaagcaaga agaucaagaa gggcaguaac acuaccaagu      360

cauagcacua gaaaauuaca aacaagaagc caaacauggu uagaaagccg agaauauaca      420

aaacauuuaa uaaaaguaga gaauuggaua uuuagaaauc caggauuugc cuuaguagca      480

guugcaauag cauggcuacu uggaucaucc acuagucaaa aaguaauaua ucuaguaaug      540

auauuauuaa uagcaccagc auauaguauu agauguauag gaguuucaaa uagagacuuu      600

guugaaggua ugucuggagg aacaugggua gacguagucu uagaacaugg aggaugugua      660

acaguaaugg cacaagauaa accuacagua gauauagaac uaguaacaac aaccguuagu      720

aauauggcag aaguaagguc auauuguuac gaagcaucaa uaucugacau ggcaagugau      780

agucgaugcc caacacaagg ugaagcguau uuggauaaac aaucagauac acaauaugua      840

uguaaaagaa cucuaguaga uagagguugg ggcaacggcu guggauuauu uggaaaggga      900

agccuaguaa caugugcaaa auucacuugc aguaaaaaaa ugacaggaaa aucaauacaa      960

cccgagaacc uagaauauag aauaauguua ucaguacaug ggucgcagca cucaggaaug     1020

auaggauaug aaacagauga aaauagggcu aaaguugagg uuacgccaaa uucuccacgc     1080

gcagaagcaa cauuaggugg auucggcuca cuaggacuag auugugagcc aagaacagga     1140

uuagacuuua gugaucuaua uuaucuaaca augaauaaua aacauugguu aguacauaaa     1200

gaaugguuuc augacauacc auuaccaugg cacgcaggag cugauacagg cacuccgcau     1260

uggaauaaua aagaagcacu cguagaguuu aaagacgcac acgccaaacg acaaacagug     1320

guuguacuag gaucacaaga aggagcaguu cacacagcac uagcaggagc acuagaagcc     1380

gaaauggaug gagcuaaagg uaaauuauuu aguggacauc ugaaaugcag acuaaaaaug     1440

gacaaacuua gauugaaagg uguaaguuau aguuuaugca ccgcagccuu uacuuuuaca     1500

aaaguaccag cagaaacacu ucauggcaca guaacaguug aaguacaaua ugcugguaca     1560

gauggaccuu guaaaauucc uguacaaaug gcagucgaca ugcaaacauu aacaccaguu     1620

ggacgccuaa uaacggcuaa uccaguaaua acugaaagca cagagaauag uaaaaugaug     1680

uuagaauuag aucccccauu cggagauagu uacauuguaa ucggaguugg agauaaaaaa     1740

auaacgcauc auuggcacag aagcgguuca acaauaggaa aagcauuuga agcaacaguc     1800

agaggugcga aaagaauggc aguccucggc gauacagcau gggauuucgg uucaguggga     1860

ggaguauuca auucucuagg gaaagguaua caucaaauau uuggugcagc cuuuaaauca     1920

uuauuuggag gaaugaguug guuuucccaa auauuaauug gaacauuauu agugugguua     1980

gggcucaaca caaaaaacgg aaguauauca cuaacuuguu uagcacuagg aggaguaaug     2040

auauuuuugu caacugcugu aagcgcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9267
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 3 opt

<400>  9267
gggagaaagc uuaccaugac aagcguagga auaguagguc uauuacuaac gacagcaaug       60

gcagcagaag uaacuagaag aggaucggca uauuauaugu auuuagacag aaaugaugcg      120

ggggaagcaa uaucuuuucc gacaacauua ggaaugaaua aauguuauau acaaaucaug      180

gaccuuggac auauguguga cgcgacaaug ucauaugaau guccaauguu ggaugaagga      240

guagaaccag augauguuga uuguuggugu aacacgacau cuacaugggu aguauauggc      300

acuugccauc auaaaaaagg agaagcgaga agaagcagaa gagcagugac acuaccuagu      360

cauucaacaa gaaaauuaca aacaagauca caaacauggu uagaaucaag agaauacaca      420

aaacacuuaa uucgaguuga aaacuggaua uucagaaacc caggauuugc cuuagcugcu      480

gcagcaauag caugguuauu aggcaguagu acaagccaaa aaguaauaua ucuaguaaug      540

auccuauuaa uagccccagc auauaguaua agauguauug guguaucuaa ucgagauuuu      600

guagaaggaa ugucaggagg aacaugggua gacguaguau uagaacacgg aggauguguc      660

acaguaaugg cacaagacaa accaacagua gauauugaau uagucacaac uacaguaagc      720

aauauggcag aaguaagaag uuauuguuau gaagccucca uaucagauau ggcaagugac      780

ucaagauguc cgacucaagg agaagcuuau cuugauaaac aaagcgauac ucaguacgua      840

ugcaaaagaa cacuuguuga uagaggcugg ggaaacggau gcggauuauu cggaaaagga      900

agccugguaa caugcgcuaa auuugcaugu aguaaaaaaa ugacaggaaa aagcauacaa      960

ccagaaaauc uagaauaucg aauaauguua ucaguacacg gcucacagca uucaggaaug     1020

auuguuaacg acacaggaca cgaaacugac gagaauagag caaaaguuga aauaacacca     1080

aacucaccga gagcagaagc aacauuagga ggcuucggca gccuaggacu agacugugaa     1140

ccacgaacag gcuuagacuu uagugaucua uauuaccuca cuaugaauaa caaacauugg     1200

uuaguucaca aagaaugguu ccaugauaua ccuuuaccau ggcaugcagg ugccgauacu     1260

gguacaccac acuggaacaa caaagaagca uuaguagaau uuaaagaugc acaugcaaaa     1320

agacaaacag uuguuguauu aggaucacaa gaaggagcag uacacacagc guuagcaggg     1380

gcacuagaag cagaaaugga cggggccaaa ggacggcuaa guucaggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu aagacuaaaa ggaguaucgu acucgcugug cacagcagcc     1500

uuuacauuca caaaaauucc ugccgaaaca cuacacggaa cuguaacagu agagguacaa     1560

uacgcaggua cugauggacc auguaaagua ccagcgcaaa uggcaguaga uaugcaaaca     1620

uugacaccag uaggaagauu aauaacagca aauccaguaa uaacagaauc uacagaaaac     1680

ucaaaaauga ugcuugaauu agauccaccu uuuggagaua guuacauagu aauaggggua     1740

ggagaaaaaa agauaaccca ccauuggcau agaucaggau caaccauagg gaaagcauuu     1800

gaagccacug ucagaggagc uaaaaggaug gcaguuuuag gagacacagc augggauuuc     1860

ggaagcgugg guggcgcacu caauagucuu ggacgaggaa uacaucaaau auuuggugcc     1920

gcauucaaaa gucuuuuugg aggaaugagu ugguuuucuc agauacuaau uggaacauua     1980

uuaauguggc uaggauuaaa uacaaaaaau ggaaguauuu cauugaugug uuuagcacua     2040

ggaggaguau uaauauuucu aaguacggca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9268
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 3 opt

<400>  9268
gggagaaagc uuaccaugac aagugucgga auagugggau uacuuuuaac aacagcaaug       60

gcugcugaag uaacuagaag agguucagca uacuacaugu acuuagacag aaaugaugca      120

ggagaagcaa uaaguuuucc gacaacauug ggaaugaaua aauguuauau acaaauaaug      180

gauuuaggac auauguguga ugcaacaaug ucauaugaau gucccauguu agacgaaggc      240

guagaaccag augaugugga uugcuggugc aauacaacau caacuugggu agucuacgga      300

acaugucauc acaagaaagg ugaagcaaga agaagcagac gagcaguaac auuaccuagu      360

cauagcacua gaaaguuaca aacacggagu caaacuuggu uagagaguag ggaauauaca      420

aagcauuuaa uaagaguaga aaacuggaua uucagaaauc caggucuagc ucuagcugca      480

gcugcaauug cauggcuauu aggaucaagu accucacaaa aagugauaua uuuaguaaug      540

auuuuacuaa ucgcaccagc auauucaaua agaugcauag gaguuagcaa ucgagauuuc      600

guagaaggaa ugaguggagg aacaugggua gauguuguau uagaacacgg uggauguguu      660

acagugaugg cgcaagauaa accuacagua gauauagaau uaguuacaac aacuguuagc      720

aauauggcgg aaguaagaag uuauuguuac gaagccagua uaagugauau ggcaucggac      780

aguagaugcc ccacacaagg ugaagcauau cuagauaaac aaucagauac acaauauguu      840

ugcaaaagaa cauuaguaga cagagguugg ggaaaugguu gcggucuuuu uggaaaaggu      900

agucuaguaa caugcgcaaa auucgccugu agcaagaaaa ugacagguaa aaguauacag      960

ccugaaaacu uagaguauag aauaaugcua ucaguacacg ggagccaaca cagcggaaug     1020

auuguuaacg acacgggaca ugaaacagau gaaaacagag caaaaguaga aauuacaccg     1080

aauagcccuc gagcagaagc cacacuaggu gguuuuggaa gccuaggauu ggacugugaa     1140

ccaaggacag gucuagauuu uagugaucua uauuacuuaa caaugaacaa uaaacauugg     1200

uuaguacaua aagaaugguu ucaugacauc ccuuuaccau ggcaugcagg agcagauacc     1260

ggaacuccac auuggaacaa uaaagaagca uuaguagaau uuaaagaugc acacgcaaag     1320

agacaaacag uaguaguauu aggaagccaa gaaggagccg uacauacagc cuuagcaggc     1380

gcguuagaag cagaaaugga uggagcaaag gguagauuaa guucgggaca uuugaaaugu     1440

aggcucaaaa uggauaaauu acgauugaaa ggaguaaguu acucuuugug uacagcagcu     1500

uuuacuuuua caaaaauacc agcagaaaca uuacauggua cagucacugu ggaaguacaa     1560

uaugcaggaa cagauggacc augcaaagua ccugcacaaa uggcaguaga caugcaaaca     1620

cuuacaccgg uagguagauu aauaacagcu aauccaguaa uaaccgaaag uacagaaaac     1680

agcaaaauga uguuagaauu agacccgcca uuuggagauu cauauauagu aauuggagua     1740

ggagaaaaga aaauaacaca ccauuggcau agaucaggaa guacuauagg uaaagcguuc     1800

gaagcuacag uaagaggagc aaaaagaaug gccguguugg gcgacacugc augggacuuu     1860

ggaucgguug gaggugcacu aaauucauua gguaaaggca uucaccaaau auuuggagca     1920

gcauuuaagu cauuauuugg aggaaugagc ugguuuucac aaauacuaau uggaacguua     1980

uuaauguggc uaggauuaaa uacuaaaaau gguagcauua guuuaaugug ucuagccuug     2040

ggaggagugu uaaucuuccu uaguacagca guuagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9269
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 3 opt

<400>  9269
gggagaaagc uuaccaugac aucaguaggu aucguaggau uacuauuaac aacagcaaug       60

gcagcagaag uuacgagaag agguucagcc uacuauaugu aucuugauag gaacgaugca      120

ggggaagcua ucaguuuucc uacaacauua ggaaugaaca aaugcuauau ucaaauaaug      180

gauuuaggac auauguguga cgcuacuaug agcuaugaau guccuaugcu agacgaaggc      240

guagaaccug augacguaga cuguuggugu aauacaacau cgaccugggu aguguaugga      300

acgugucauc auaagaaagg agaagcaaga agaagcagac gagcuguaac auuaccauca      360

cauaguacaa gaaaauuaca aacacgauca caaacauggc uagaaaguag agaauauaca      420

aaacauuuaa uuagaguuga aaauuggaua uuuaggaauc cagguuuugc auuggcagca      480

gcugcaauug cguggcugcu cggcaguuca acaucacaaa aaguaauaua ucuaguaaug      540

auauugcuaa uagcuccagc uuauucaaua agaugcauag gaguuucaaa uagagacuuu      600

guugaaggca ugaguggagg cacaugggua gacguaguau uagaacacgg aggaugcgug      660

acuguaaugg cacaagauaa accaacagua gauauugagu ugguaacaac aacaguguca      720

aauauggcag aaguaagaag uuauuguuac gaagcuagca uaagugauau ggcaucagac      780

agcagauguc cuacucaagg cgaagcauau cucgacaaac aaagugauac acaauacgua      840

uguaaaagga cacuggucga cagagggugg ggaaacggcu gugguuuguu uggaaaagga      900

agucuaguaa ccugugcaaa auuugcuugu aguaaaaaga ugacgggaaa gaguauccaa      960

ccagaaaacu uggaauauag aauaauguua uccgugcacg ggagucaaca uucaggaaug     1020

auaguaaacg auacaggaca ugaaacagac gaaaauagag caaaagugga aauuacucca     1080

aauaguccaa gggccgaagc uacucuuggc ggauuuggau cauuaggauu agacugcgaa     1140

ccgaggacag gacuagauuu cagugauuug uacuaucuaa caaugaacaa caaacauugg     1200

uuaguccaua aagaaugguu ccaugauauu ccauuaccgu ggcaugcugg ugcggauacu     1260

ggaacaccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc ucaugcaaaa     1320

agacaaaccg uaguaguuuu agguagucaa gaaggagcag uacacacagc auuggcggga     1380

gcccuagaag cagaaaugga uggagcaaaa gggagacuau cuucaggaca cuugaaaugu     1440

agauugaaaa uggauaaacu cagauuaaaa gggguaucau auucauuaug uacagcugca     1500

uucacauuua caaaaauacc agcugaaaca cuacauggua cuguuacggu agaaguacaa     1560

uaugcaggca cagacggacc uuguaaaguu ccagcacaaa uggcaguaga caugcaaaca     1620

uuaacaccug uaggaagauu aauaacagca aauccaguga uaacagaauc aacagaaaau     1680

aguaaaauga uguuagaauu agauccacca uuuggcgauu cauauauagu aauaggugua     1740

ggagaaaaga aaaucaccca ccauugguau agaagcggaa gcacaauagg uaaagcauuu     1800

gaagcaacag uaagaggagc aaaaaggaug gccguucuag gagacacugc augggauuuu     1860

ggaaguguag gaggagcacu aaacucuuua ggaaaaggua uucaucaaau auuuggagca     1920

gcauuuaaau cguuauucgg aggaaugagc ugguuuucac aaaucuuaau aggaacauua     1980

cuaauguggc uuggauugaa uaccaaaaau ggaucgauau cuuuaaugug ucuagcacua     2040

gggggaguuu uaauauuucu aucaacagca gugagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9270
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 3 opt

<400>  9270
gggagaaagc uuaccaugac gucuguugga auaguuggau uauuacuaac aacagcaaug       60

gcagcagaag uuacaagaag aggcucagcc uauuacaugu acuuagauag aaaugacgca      120

ggagaagcua uuagcuuucc gacuacucua ggaaugaaua aaugcuacau acaaauaaug      180

gacuuaggac acaugugcga ugcaacaaug aguuacgaau guccaaugcu agacgagggu      240

guugaaccgg augauguaga uuguuggugu aacaccacaa gcacuugggu gguauaugga      300

acgugucacc acaaaaaagg agaagcacgu agaucacgaa gggcaguaac acucccauca      360

cauaguacua gaaaauuaca aacaagaagu caaacguggu uagaaucaag agaauacacc      420

aaacacuuaa uuagaguaga aaauuggaua uuucgaaauc cagguuuugc ucuagcagca      480

gcagcaauag caugguuauu aggaagcucg acaagucaaa aaguaauaua uuuaguaaug      540

auacuauuaa uagcuccggc uuauucaaua agauguauag gaguaucaaa uagagacuuu      600

guagaaggaa ugucuggagg aacuuggguu gaugugguau uagaacaugg gggaugugua      660

acaguaaugg cccaagauaa accaacaguu gauauagaac uuguuacaac aaccguaagu      720

aacauggccg aaguaagauc cuauugcuau gaagcaucaa uaucggauau ggcaagugau      780

ucaagauguc cuacucaagg agaagccuac uuagacaaac aaagcgauac acaauaugua      840

ugcaaaagga cacuaguaga ucgugguugg ggaaacggau guggauuguu cggaaaaggu      900

agccucguaa caugugcaaa auuugcuugu aguaaaaaaa ugacaggaaa aucaauacaa      960

cccgaaaauu uagaauauag aauaaugcua aguguccacg ggagccaaca uagcgguaug     1020

auaguuaacg auacaggaca ugaaacugau gaaaauaggg caaaagucga aauaacacca     1080

aauaguccaa gagcagaagc aacauuaggu ggauuuggau cuuuaggacu agacugugaa     1140

ccaaggacug guuuagauuu uagcgacuua uauuaucuaa caaugaacaa uaaacauugg     1200

uuaguacaua aagaaugguu ccaugauaua ccauuaccau ggcacgcagg agcagauacc     1260

gguaccccgc acuggaauaa uaaagaagca cuaguugaau uuaaagacgc acaugcaaaa     1320

cgucagacag uaguaguauu aggaagucaa gaaggcgcag uacauacagc auuagcagga     1380

gcacuugaag cggaaaugga uggugcaaaa ggaagauuaa guucaggaca uuugaagugu     1440

cgguuaaaaa uggacaaacu aagauuaaaa ggaguaucau auucgcuaug uacagcugcu     1500

uuuacauuca cuaaaauccc agcugaaaca cuacauggaa caguaacagu agaaguucaa     1560

uaugcaggua cugacggacc augcaaaguc ccagcacaaa uggcagugga uacacagacc     1620

uuaacacccg ugggaagacu uauuacugca aauccaguaa uaacugaauc aacugaaaau     1680

ucaaaaauga uguuagaauu agauccacca uuuggagacu cguauaucgu aauuggugua     1740

ggagaaaaaa aaauaacuca ucacuggcau agaucaggau caacaauagg aaaagcauuc     1800

gaagcuacag uaagaggcgc aaaaagaaug gcaguccuag gagauacugc augggacuuc     1860

ggaagcguag gaggcgcacu uaauucucua gguaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaaa gcuuauuugg aggaaugucc ugguuuucac aaauacuaau aggaacuuug     1980

uuaauguggc uuggucuuaa uacaaaaaau gggucaauaa guuuaaugug ccuagcuuua     2040

ggaggggucu uaauauuucu uuccacugca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9271
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 3 opt

<400>  9271
gggagaaagc uuaccaugac aucaguuggc auuguaggau uacuauuaac aacagcuaug       60

gcagcagaag uaacacgaag gggaucagca uauuauaugu aucuagauag aaacgaugca      120

ggagaagcaa ucagcuuccc aacaacacua gggaugaaua aauguuauau acaaauuaug      180

gauuugggcc acauguguga cgcaacaaug aguuaugaau gcccaaugcu ugacgaagga      240

guugaacccg augauguaga cugcuggugu aauacaaccu caacuugggu aguuuaugga      300

accugucauc acaagaaagg agaagcucga agaucaagac gagcaguaac auuaccuagu      360

cauucaacga gaaaauuaca aacuagauca caaacuuggc uugaauccag agaauacaca      420

aaacaucuaa uaagaguuga aaacuggauc uuuagaaacc cugguuuugc cuuagcagcg      480

gcugcaauag caugguuacu aggaucauca acaagucaga aagucauaua uuuagucaug      540

auauuacuaa uagcuccugc auacucuaua agauguauag gugugaguaa uagagacuuu      600

guggaaggaa ugucuggugg aacaugggug gacguaguuu uagagcacgg gggaugcguu      660

acaguaaugg cccaagauaa accaacaguu gauauugagc uaguaacaac gaccguaagc      720

aauauggcag aaauaagauc auauugcuau gaagccucaa uuagugacau ggcuagcgau      780

agcagaugcc caacacaggg agaagcauac cuggacaaac aaucagacac acaauaugua      840

ugcaaaagaa cauuaguaga cagaggaugg ggaaaugguu guggacuauu uggaaaaggg      900

ucauuaguaa caugcgcaaa auuugcaugu ucaaagaaaa ugaccgguaa aucaauacaa      960

ccagaaaauu uagaauacag aauaaugcua ucaguacacg guagucaaca uagcgggaug     1020

auuguaaacg auacaggaca cgaaacagau gaaaaucgcg caaaaguaga aauaacgcca     1080

aauaguccua gagcugaagc aacauugggu ggauuuggga guuuaggacu agauugcgaa     1140

ccuagaacag gacuagauuu uagugacuua uauuaucuca caaugaauaa uaaacacugg     1200

uuaguucaca aagaaugguu ucaugauaua ccauuaccau ggcaugcagg agcagauaca     1260

gguacacccc auuggaacaa uaaagaagcu cuaguagagu uuaaagacgc acaugcaaaa     1320

cgacaaacag uaguaguuuu agguagucaa gaaggcgcag ugcauacagc acuagcagga     1380

gcauuagaag cagaaaugga cggugcgaaa ggucgauuaa guaguggaca uuuaaaaugc     1440

cgacuaaaaa uggacaaauu acgauuaaaa gguguaucau acucauuaug uaccgcugca     1500

uucacuuuua caaaaauacc ugcagaaaca uugcacggaa cuguaacagu agaaguacaa     1560

uaugcgggua cagauggacc augcaaaguu ccagcccaaa uggcugugga uaugcaaaca     1620

cuaacaccag uagguagacu uauaacggca aauccaguaa uaacagaaag uacagaaaau     1680

ucaaaaauga uguuagaauu agauccccca uuuggagaca gcuauauagu uauaggagua     1740

ggagagaaaa agauaacuca ucauuggcau cgaagcgguu caacaauugg aaaagcauuu     1800

gaagcaacug uaagaggagc aaaaagaaug gccgucuuag gggacacugc uugggauuuc     1860

ggaucaguag gcggugcauu aaauucacua ggaaaaggua uucaccaaau auuuggagca     1920

gcauucaaau cauuauucgg aggcaugagu ugguucaguc aaauacuaau aggaacauua     1980

cuaauguggc uuggacuaaa cacuaaaaau gguucaauaa guuuaaugug ucuugcauua     2040

ggcggcguac uuauauuuuu aucaacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9272
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 3 opt

<400>  9272
gggagaaagc uuaccaugac cucaguaggu auaguuggau uacuacuaac aaccgcaaug       60

gcugcagaag uaacaagaag aggaucagca uauuauaugu auuuagacag aaacgaugca      120

ggggaagcga uaucuuuucc aacaacuuua gggaugaaua aauguuacau acaaauaaug      180

gaccuagguc acauguguga ugcaacuaug ucauaugaau gcccaaugcu cgaugaagga      240

guagaaccag acgacguaga cuguuggugu aauacaacuu caacaugggu aguauauggc      300

acaugccauc auaagaaagg agaagcaaga agaucacgua gagcaguaac auugccauca      360

cauucuacca gaaaacuaca aacaagaagc caaacauggu uagaaucaag agaauacaca      420

aaacacuuaa uaagaguaga aaauuggauu uuuagaaacc cagguuucgc uuuagcugca      480

gcagcuauag cuuggcuauu agggaguagc acaucacaaa aagucauaua ucuaguuaug      540

auccuccuga uagcaccagc auauaguaua cgauguauag gaguaucaaa cagggacuuc      600

guugaaggaa ugaguggagg aacuugggua gauguuguuc uagagcaugg aggcugugua      660

acuguaaugg cacaagacaa accaacagua gacauagagc uaguaacaac uacuguaagc      720

aauauggcug aaguaagauc uuauugcuau gaagcaagca uaucagauau ggcaagugac      780

agucgauguc caacccaagg agaggcuuau uuagauaaac aauccgauac ucaauauguu      840

uguaaacgaa cccuagugga cagaggaugg gguaacggau guggacuauu ugggaaagga      900

agccucguga caugugcaaa auuugcuugu ucgaaaaaga ugacaggaaa aucaauacaa      960

ccugaaaauc uagaauauag aauaaugcua ucuguacacg gaagccaaca uaguggaaug     1020

auaguaaaug acacagguca ugaaacagac gagaacagag caaaaguuga aauuacgccc     1080

aauagcccuc gagcagaagc uacacuaggg ggauuugguu cucuaggacu agacugugaa     1140

ccaagaacag gccuagauuu uucagaccua uauuauuuaa caaugaauaa uaaacacugg     1200

cuaguccaua aagaaugguu ccacgauaua ccauuacccu ggcacgccgg agcagacaca     1260

ggaacaccuc acuggaauaa uaaagaagcc cuuguagaau uuaaagaugc acacgcaaaa     1320

cgucaaaccg uaguaguauu aggcagucaa gaaggagcag uucauacagc auuagcugga     1380

gcauuagaag cagaaaugga cggagcaaag ggcagacuau ccucaggaca ucuaaaaugc     1440

agauugaaaa uggauaaacu acgauuaaaa ggaguaaguu auucacugug uacagcagca     1500

uuuacguuua caaaaauacc agcagaaaca uuacacggaa cagucacagu agaaguucaa     1560

uaugcaggaa cagacggucc auguaaagua uuagcacaaa uggcuguaga uaugcaaaca     1620

cuaacaccug uaggaagauu aauuacugca aauccuguua ucacugaaag cacagaaaac     1680

ucaaaaauga ugcuugaauu agauccacca uuuggcgauu cauacaucgu cauuggagua     1740

ggagaaaaga aaauaacgca ucacuggcac cgaaguggau caacaauagg uaaagcauuu     1800

gaagcaacag uacguggagc uaaaagaaug gcaguuuuag gagauacagc uugggauuuu     1860

ggaagugucg guggagcacu aaacucacua ggaaaaggaa uacaucaaau auuuggagca     1920

gcauucaaau cauuauuugg aggcauguca ugguuuaguc aaauuuuaau aggaacucua     1980

cuaauguggu uaggauuaaa cacuaaaaac gguucaauau ccuuaaugug ucuagccuua     2040

ggaggaguuc uuauauuucu cagcacagcu guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9273
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 3 opt

<400>  9273
gggagaaagc uuaccaugac gaguguagga auaguaggau uguugcuaac aacagcuaug       60

gcugcagaag uaacuagaag aggcagugcc uacuacaugu acuuagacag aaaugacgcu      120

ggugaagcaa uuagcuuucc aacaacacua ggaaugaaca aauguuauau acaaauaaug      180

gacuuaggcc auauguguga ugcaaccaug ucauacgagu guccaacauu agacgagggc      240

guugaaccag acgacguaga uugcuggugu aauaccacuu caacaugggu aguauauggu      300

acuugucacc auaagaaagg ugaagcaaga agaucacgua gagcagugac cuuaccuuca      360

cauucuacaa gaaaauuaca aacaaggagu caaacauggc uugaaucaag agaguauaca      420

aaacaccuaa uaaggguuga aaauuggaua uuucgaaacc cgggguuugc acuagccgca      480

gcugcaauag cauggcuccu aggaaguuca acuagccaaa aaguaauaua ccuaguaaug      540

aucuugcuua uagccccagc auacaguaua agauguauug gagugaguaa uagagauuuc      600

guagaaggaa ugucaggagg gacuuggguu gauguaguau uagaacaugg aggaugcgua      660

accguaaugg cacaagauaa accgacgguu gauauagaau uaguaaccac aacaguaagu      720

aauauggcug agguaagaag uuacuguuau gaggcaucaa uaucagacau ggccagcgac      780

agucguuguc caacgcaagg agaagccuau uuagacaaac aaucagauac acaauaugua      840

uguaaaagaa cucuaguuga uagaggaugg ggaaacggcu gcggacuuuu uggaaaagga      900

ucauuaguga caugcgcaaa auuugccugu aguaagaaaa ugacagguaa aucuauacaa      960

ccugaaaauu uagaauauag aauuauguua ucaguacacg gaucacaaca uucggggaug     1020

auaguaaaug auaccggaca cgaaacagau gaaaauagag cuaaaguuga aaucacacca     1080

aacaguccaa gagcagaagc aacauuaggc ggauucggau cacuaggccu agauugugaa     1140

ccaagaacag gacuagauuu uagcgaucuu uauuacuuaa caaugaauaa uaaacauugg     1200

uuaguucaua aagaaugguu ucaugauaua ccccuuccau ggcaugccgg agcagauacu     1260

ggaacaccac acuggaauaa caaagaagca uuagucgaau uuaaggaugc acaugcaaaa     1320

agacaaacug uuguaguauu aggaucacaa gaaggagcag uacauacagc uuuagcugga     1380

gcacuagaag cugaaaugga cggagcaaaa ggacgauuaa gcucuggaca ccuaaaaugu     1440

agacuaaaaa uggauaaacu aagacucaaa ggaguuucuu auucguuaug uacugcagca     1500

uuuacuuuua caaaaauucc agcagaaacg cuacauggaa caguaacugu agaaguacaa     1560

uaugccggaa cugauggacc auguaaaguc ccagcacaaa uggcaguuga uaugcaaaca     1620

cuaacuccag uaggaagauu aauaaccgcu aauccaguca uaacagaauc aacagaaaau     1680

ucaaaaauga uguuagaauu ggauccuccc uuuggugaca guuacauagu aauaggagua     1740

ggagaaaaga aaauaacaca ucacuggcau agaaguggau caacuauagg uaaagcauuu     1800

gaagcuacag uacgaggagc aaaaagaaug gccguucuug gagacacagc uugggacuuu     1860

ggaaguguag gaggagcucu aaauagcuua ggaaagggua uacaucaaau auuuggagcc     1920

gcuuuuaaaa gucuauucgg aggaauguca ugguuuucac aaauauuaau aggaacacua     1980

cuaauguggu uaggacuaaa uacgaaaaau ggcagcauua gcuuaaugug uuuagcacua     2040

ggaggaguuc uaauauucuu aucaacagcc guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9274
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 3 opt

<400>  9274
gggagaaagc uuaccaugac aucaguagga auagugggau uacuuuuaac aacggcaaug       60

gcagcugaag uaacaagaag aggcagugcu uauuauaugu auuuagacag gaaugaugca      120

ggugaagcaa uuaguuuucc uacaacuuua ggcaugaaca aauguuacau acaaauaaug      180

gaucuaggac auaugugcga ugcaacaaug ucauaugagu gcccuauguu agaugaaggu      240

guugaaccag acgaugugga uugcuggugu aauaccacca gcacuugggu aguauacgga      300

acaugucauc auaaaaaagg cgaagcaaga agaaguagga gagcuguaac ucucccuagu      360

cauucuacga gaaaauuaca aacaagauca caaacuuggc uugaaucaag agaauauaca      420

aaacauuuaa uacgaguaga aaacuggaua uuuagaaauc cagguuucgc acuggcagca      480

gcagcaauag caugguuacu aggaucauca acaagccaaa aaguaauaua cuuaguaaug      540

auucuguuaa uagcaccagc guauagcauc cguuguauag guguaucaaa uagagauuuu      600

gucgaaggaa ugaguggagg aacauggguu gauguaguuc uagaacacgg aggcugcguc      660

acagucaugg cacaagauaa accaacggua gauauugaau uaguaacaac aaccgucagc      720

aacauggcag aaguaagauc auacuguuau gaagcaucaa uauccgauau ggcaucagac      780

ucuagauguc caacucaggg agaggcauau uuagauaaac aaucagauac gcaauaugua      840

uguaaaagaa cauuaguaga uagagggugg ggcaauggau guggauuauu uggaaaggga      900

ucuuuaguua cuugugcaaa auuugcaugc ucaaaaaaaa ugacaggaaa aaguauucaa      960

ccagaaaauu uagaauaccg aauaaugcuu aguguacacg gaagucaaca uucaggaaug     1020

aucgucaaug acacagggca cgaaacagac gaaaacagag caaaaguaga aauaacaccu     1080

aauucuccaa gagcagaagc aacuuuaggu ggauucggaa gcuugggccu ugauugugaa     1140

ccaagaaccg ggcuugauuu uagcgauuua uacuauuuaa caaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ccaugauaua ccucuaccau ggcacgcagg ugcagauaca     1260

ggaacaccac acuggaacaa uaaagaagcc uuaguugaau uuaaagaugc acaugcgaaa     1320

agacaaacag ucgucguacu aggaagucaa gaaggagcag uacauacugc ucuugcaggg     1380

gccuuagaag cagagaugga uggagcaaaa ggaagauuau cuucgggaca ucucaaaugu     1440

agauuaaaaa uggauaaauu aagacuaaaa ggugucuccu acagucuaug uacggcagca     1500

uucacauuua caaaaauacc ugcagaaacc uuacauggaa caguaacagu cgaaguacaa     1560

uaugcaggaa cagauggucc auguaaagua ccagcacaaa uggcaguuga uaugcagaca     1620

uuaacacccg uaggaagauu aauaacagca aauccaguca uaacugaauc aacagaaaac     1680

aguaaaauga uguuagaauu agauccgcca uucggagaua guuauauagu uauuggaguc     1740

ggugaaaaaa aaauaacuca ccauuggcac agaucaggau caacaauagg aaaagcauuu     1800

gaagcaacag uaagaggagc aaaaagaaug gcaguguuag gagauacugc augggauuuu     1860

ggaaguguag gaggagccuu gaauucauua gguaaaggua uacaccaaau auuuggggcc     1920

gcauuuaaaa gcuuauuugg aggaaugagu ugguuuucac aaauucuaau aggaacauua     1980

uuaauguggc uuggauuaaa uacuaaaaac gguucuauuu cacuaaugug uuuagcauua     2040

ggcgggguuc uaauauuucu uucuacgguc guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9275
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 3 opt

<400>  9275
gggagaaagc uuaccaugac aucaguuggu auaguuggac uacuauuaac aacagcgaug       60

gcagcugaag uaacaagaag aggaucagca uauuacaugu acuuagauag gaacgaugcu      120

ggugaagcaa uuucauuucc uacaacacua ggaaugaaua aauguuauau acaaauaaug      180

gaccuagguc auaugugcga ugcuacuaug ucuuaugaau gcccaaugcu agaugaagga      240

guagaaccag augauguaga cuguuggugu aacacaacca gugcaugggu aguauauggu      300

accugccauc acaagaaagg agaagcuaga agaucuagac gagcuguaac auuaccuagc      360

cauucaacga gaaaauuaca gacaagacca caaacuuggc uagaaucuag agaauacacc      420

aaacaucuaa uaaggguaga aaauuggaua uuuagaaauc cagguuuugc acuagcugcc      480

gcagcaauug cuugguuacu agguucauca acaucucaaa aaguaaucua ucuaguuaug      540

aucuuacuaa uagcuccugc auauaguaua agaugcauag gaguaagcaa cagagauuuu      600

guagaaggua ugucaggugg aacauggguu gauguaguac uugaacacgg uggaugugug      660

acuguaaugg cacaggauaa acccacagug gacauagaau uaguaacaac aacuguguca      720

aauauggcug aaguaagauc auacugcuau gaagcaucaa uuagugauau ggcauccgau      780

aguagauguc cuacacaagg agaagcauau cuagauaaac aaagugacac acaauauguc      840

uguaaaagaa cguuaguuga uagaggaugg ggaaauggau gcggauuauu uggaaaagga      900

ucacuuguaa ccugugcaaa auuugcaugu ucaaagaaaa ugacaggcaa aaguauacaa      960

ccagaaaauc uagaauauag aauaauguua ucgguccaug gaucacaaca uagcgguaug     1020

auuguaaaug auacaggaca cgaaacagac gaaaauaggg caaaaguuga aaucacccca     1080

aacucaccca gagcagaagc aacucuggga ggauuuggca gccugggauu agauugugaa     1140

ccaagaacug gacuugauuu uagugaucua uacuaucuua caaugaacaa uaaacauugg     1200

cucguacaua aagaaugguu ccaugauaua ccauuaccuu ggcaugcagg agccgauaca     1260

ggaacaccac acuggaacaa uaaagaagca uuaguagagu ucaaagacgc acaugcaaaa     1320

aggcaaacag uaguuguacu aggaagccaa gaaggagcag uucauacugc auuagcgggu     1380

gcauuagaag ccgaaaugga uggagcgaaa gguagauuau cuucuggaca cuugaaaugu     1440

cgcuuaaaaa uggauaaacu aagacuaaaa ggaguaaguu acucauuaug cacagcagca     1500

uucacauuua caaaaauucc ugcggaaaca cuacacggaa cuguaacagu agaaguacaa     1560

uaugcuggaa cugauggacc auguaaaguu cccgcacaaa uggcaguuga caugcaaaca     1620

uuaacaccgg uaggaagauu gauuacugcg aacccuguua uaacagaaag uacggaaaau     1680

aguaaaauga ugcuagaauu agauccaccu uuuggagaua guuauauagu aauaggagua     1740

ggugaaaaga aaauaacaca ucauuggcac agaagcggau caacaauagg aaaagcauuu     1800

gaagcuacag uuagaggagc uaaacgaaug gccguacuug gagacacagc cugggauuuu     1860

ggcuccguag gaggagcauu aaacagucua gguaaaggca uacaucaaau auuuggagca     1920

gccuuuaaaa guuuauuugg agguauguca ugguuuucac aaauacuaau aggaacauua     1980

uuaauguggc uaggacuaaa cacuaaaaau ggaucgauaa gccuuaugug cuuagcacua     2040

ggagguguuu uaauauuucu aagcacagca gugucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9276
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 3 opt

<400>  9276
gggagaaagc uuaccaugac aucuguggga auaguagguu uauuauuaac uaccgcaaug       60

gcugccgaag uaacaagaag aggaucagca uauuauaugu accuugauag aaaugaugca      120

ggagaagcaa uaucuuuucc aacaacauua gguaugaaua aaugcuacau acaaauuaug      180

gauuuaggac auauguguga ugcaacgaug ucauaugaau guccaaugcu ugaugaagga      240

guugaaccug acgauguaga uuguuggugu aauacaacaa gcaccugggu uguauaugga      300

acaugucauc auaaaaaagg agaagcucga agaaguagaa gagcaguaac ccuaccgagc      360

cacagcacua gaaaauuaca aacuagguca caaaccuggc uagaaaguag agaauacaca      420

aaacaucuua uaagaguaga aaauuggauc uuuagaaauc cagguuucgc acuagcagca      480

gcagcaauag cauggcuacu agguucgucc acaucaaaaa aaguaauaua uuuaguaaug      540

auauuacuaa uagcaccagc auauucaaua agauguauag guguaaguaa uagagauuuc      600

guugaaggaa ugaguggagg aacaugggua gauguagucu uagaacacgg aggaugugug      660

acuguuaugg cacaagacaa accaacagua gacauagaac uuguaacgac aacaguaagc      720

aauauggcag aaguacguag uuauugcuau gaagcaucaa uuucagauau ggcaucagau      780

uccagauguc caacacaagg agaagcuuau uuagauaaac agucagauac gcaauauguu      840

ugcaaacgaa cccuaguuga cagagggugg ggcaacggau gcggauuguu cggaaaagga      900

ucguuaguaa cuugugccaa auucgcaugu ucuaaaaaaa ugaccggaaa aucaauacaa      960

ccagaaaauu uagaauauag aaucaugcua agcgugcaug gcucacaaca cucuggcaug     1020

auuguaaaug auacaggaca ugaaacugac gagaauagag cuaaaguaga aauaacaccg     1080

aauucaccca gagcagaagc aacauuagga ggauucggau cacugggauu agauugcgaa     1140

ccacgaacag gauuagauuu cagugaccuu uauuauuuaa caaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ccacgacaua ccauuaccuu ggcaugcagg cgccgauaca     1260

ggaacaccac auuggaauaa uaaagaagcu cuaguagaau uuaaagaugc acaugcaaaa     1320

aggcaaacag uagucguucu aggaucacaa gaaggagcag uacauacagc auuagcagga     1380

gccuuagaag ccgaaaugga uggcgcaaaa ggaagacuaa gcagcggaca uuugaaaugu     1440

cgguuaaaga uggacaaguu aagguuaaaa ggcguuagcu acaguuuaug uacagcagca     1500

uuuaccuuca caaaaauccc ugccgaaaca uuacauggaa caguuacagu agaaguucaa     1560

uaugccggga cugacggacc auguaaagua ccagcucaaa uggcaguaga caugcaaaca     1620

uuaacaccug uuggaagacu aauaacagca aauccaguga uaacugagag uacagaaaac     1680

aguaaaauga uguuagaauu ggacccucca uucggugaca gcuauaucgu aauaggagua     1740

ggugaaaaaa aaauaacaca ucacuggcac agaucaggau caacaauagg uaaagcauuc     1800

gaagcaacag uacguggagc aaaacgaaug gcgguucuag gagauacugc gugggauuuc     1860

ggaagcgucg gaggggcauu aaacucauua gggaaaggaa uucaucaaau auuuggugcc     1920

gcauuuaaaa gccuauuugg agguaugagu ugguuuaguc aaauauuaau uggaacacuc     1980

cuuauguggc ucggauuaaa uacuaaaaac ggaucuauua guuuaaugug uuuggcauua     2040

ggugguguac uuauauuuuu aaguacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9277
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 3 opt

<400>  9277
gggagaaagc uuaccaugac gaguguaggu auaguaggau uauuacuaac cacagcuaug       60

gccgcagaag uaaccagaag aggaagugca uacuauaugu acuuagaucg gaaugaugca      120

ggggaagcca uaagcuuucc aacaacucua gguaugaaua aaugcuauau acaaauaaug      180

gacuuaggac auauguguga ugcgacaaug agcuaugaau guccaaugcu agacgaaggc      240

guagaaccag augauguuga cugcuggugu aacacaacaa guacaugggu aguauacggg      300

acaugucacc auaaaaaagg cgaagcgcga agaucaagaa gagcaguuac auuaccaagu      360

cauuccacua gaaaauuaca aacccguuca caaacauggc uugaaucacg agaauacaca      420

aaacaucuua uucguguaga aaacuggaua uuccgaaauc cgggauuugc acuagcagca      480

gcagcaaucg caugguuauu aggaaguucu acaagacaaa aaguuauaua ccucguaaug      540

auacuuuuaa uagcaccagc cuauagcaua agauguauag gaguauccaa cagagauuuc      600

guugaaggaa ugaguggagg aacaugggua gacguagucu uagaacaugg aggaugugua      660

acaguaaugg cucaagauaa accuacaguu gauauagaac ucguaacaac uacaguaagu      720

aauauggccg aaguccguuc auauuguuau gaagcaucaa uaucagauau ggcgagcgac      780

aguagauguc cuacccaagg agaagcauau uuagacaaac aaagugauac acaauauguc      840

ugcaaaagaa cauuagugga cagagguugg gguaauggau gcggacuauu cggaaaagga      900

aguuuaguaa caugugccaa auuugccugu ucaaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaacu uagaauauag aauuauguua ucaguccaug gcucacaaca cagugggaug     1020

auagucaacg acacaggaca ugaaacagau gaaaauagag cuaaaguaga aauuacacca     1080

aauucacccc gagcggaagc aacacuagga ggauuuggau cacuaggauu agauugugaa     1140

ccuagaacag guuuagacuu uagugauuua uacuaucuua caaugaacaa uaaacacugg     1200

uuaguacaca aagagugguu ccaugacaua ccacuuccau ggcaugcagg agcagacacg     1260

gggacgccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc acaugcgaaa     1320

agacaaacgg uaguaguacu agguagccaa gaaggagcag uacacacagc cuuagcaggu     1380

gcauuagaag cagaaaugga cggagcuaaa ggacgguuau caagugggca cuuaaaaugc     1440

agacuaaaaa uggacaaauu aagauuaaaa ggaguaagcu acucauuaug cacagcagca     1500

uuuacauuca cuaaaauccc cgcagagacu uuacauggca cagugaccgu agaaguacaa     1560

uacgcaggca cagauggacc auguaaaguu ccugcacaaa uggcuguaga uaugcaaacg     1620

uuaacaccag uugguagauu aauaacugca aauccuguaa uuaccgaguc cacggaaaau     1680

ucaaaaauga ugcuagaauu agacccacca uuuggagauu cuuauauagu aauaggaguc     1740

ggagagaaga aaauaacuca ucauuggcau agaagcggaa guacaauagg aaaagcauuu     1800

gaagcaacag ucagaggagc caaaagaaug gcaguucuag gagacaccgc uugggauuuu     1860

ggaucaguag gaggugccuu aaauucucua ggaaaaggaa uacaucagau auuuggugca     1920

gcauuuaaaa guuuauucgg uggaaugucg ugguuuucac agaucuuaau aggaacgcug     1980

cuaauguggc uaggacuaaa uacaaaaaau ggaucaauau cauuaaugug ucuagcauua     2040

gguggcguac uaauauuuuu aucaacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9278
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 3 opt

<400>  9278
gggagaaagc uuaccaugac aagugucggc auaguagguc ucuuacugac aacagcgaug       60

gcagcagaag uaacaagaag aggcagugca uacuacaugu auuuagauag gaaugaugcc      120

ggggaagcaa uaucauuccc aacaacuuua gguaugaaua aauguuauau acaaauaaug      180

gauuuggggc auauguguga cgcuaccaug aguuacgaau guccaaugcu ugaugaaggu      240

gucgaaccag augauguaga uuguuggugu aauacaacua guacaugggu aguuuaugga      300

acuugucauc acaaaaaagg agaagcgaga agaagcagaa gagcugugac acuccccucc      360

cauaguacua gaaaacuaca aacacgaagc caaacauggu uagaaucaag agaauauaca      420

aaacauuuaa uaagaguaga aaauuggauu uuuagaaauc caggauuugc auuagcugca      480

gcugcaauag cauggcuccu ggguucauca acuagucaga aagugauuua uuuaguuaug      540

auacuacuaa ucgcacccgc guauucaaua agguguauag guguauccaa cagagauuuc      600

guagaaggaa ugucuggagg aacauggguu gauguaguuu uagaacacgg uggguguguu      660

acaguaaugg cucaagacaa accaacugua gauauugaac uaguaacaac uacuguaagc      720

aauauggcag aagucagaag uuauuguuac gaagccucaa uaagcgacau ggcuucagau      780

ucuagauguc caacacaagg ggaagcauau uuagauaaac aaucagauac acaauauguc      840

ugcaaaagaa cacuagucga cagaggaugg gggaacgguu gcggguuauu cggcaaagga      900

ucauuaguua ccugcgcaaa auucgcaugu agcaaaaaaa ugacggguaa aaguauacaa      960

ccagaaaauc uugaauauag aauaaugcua ucuguucacg guucacaaca uaguggaaug     1020

auagucaaug auacaggaca ugaaaccgau gaaaacagag caaaaguuga aaucacacca     1080

aauucaccuc gagcagaggc uacauuagga ggcuuuggua gucuuggauu agacugugaa     1140

ccgagaacag gauuagauuu uagugauuua uauuauuuaa caaugaauaa uaaacauugg     1200

cugguacaca aagaaugguu ucaugauauu ccacuuccau ggcacgcagg ugcagauaca     1260

gguacaccgc acuggaauaa caaagaagca uuagucgaau uuaaagacgc acaugcaaaa     1320

agacaaacag uaguuguuuu aggcacucaa gaaggugcag uccauacggc acuagcgggg     1380

gcacuagaag cagaaaugga uggugcuaaa ggaagacuaa guucaggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu aagacuaaaa ggaguaucau auucauuaug uacagcagca     1500

uuuacauuua cgaaaauucc agccgaaacc cuacacggua cuguuacugu agaaguccaa     1560

uaugcaggua cagauggacc auguaaagua ccugcgcaaa uggcaguaga uaugcaaaca     1620

cuuacaccag uaggaagauu aauaacugcg aauccaguaa uaacggaauc cacagaaaau     1680

ucaaagauga uguuagaauu agauccaccu uuuggagauu cauacauagu aaucggaguu     1740

ggugaaaaaa aaauaacaca ucauuggcau agaucaggcu caacaauagg aaaagccuuu     1800

gaggcaacag uaagaggcgc caaaagaaug gcaguccuag gugacacagc augggauuuu     1860

ggaucuguag gaggagcauu aaauucccua ggaaaaggaa uacaccaaau uuuuggagcc     1920

gcuuuuaaau ccuuauuugg aggaauguca ugguuuucac aaauucuaau uggaacacua     1980

uugauguggu uaggcuuaaa cacaaaaaac ggaagcauca guuuaaugug ucuggcacuc     2040

ggaggaguac uaauauuccu aucaacagca guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9279
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 3 opt

<400>  9279
gggagaaagc uuaccaugac uucuguagga auaguaggcc uauuguuaac aacugccaug       60

gccgcagaag ucacaagaag gggcagcgca uacuauaugu auuuagauag aaacgaugca      120

ggagaggcaa uuucauuucc aacaacacua ggaaugaaua aauguuauau acaaauaaug      180

gaucuugguc auauguguga cgcaacaaug ucguaugaau guccuaugcu ugacgaaggu      240

guugaaccag augauguaga uuguuggugc aauacaacau caacuugggu aguauacgga      300

accugucauc auaaaaaagg agaggcaaga agaucaagaa gagcaguuac gcuaccuagu      360

cauucgacaa ggaaauuaca aacaagaagu caaacauggc uagaaagcag agaauacacu      420

aaacaccuaa uaagaguaga aaacuggaua uuuagaaauc cuggauuugc acuagcagcu      480

gcggcaauag cauggcuauu gggcucauca acaagccaaa aaguaauaua ucuagucaug      540

auacucuuaa uagcaccagc auacagcuua agauguauag gagucagcaa ucgagauuuu      600

guagagggua ugaguggagg uacaugggug gauguaguuc uugaacaugg agggugugua      660

acaguaaugg cucaagauaa accaacagua gauauagagu ugguaacgac aacagucagc      720

aauauggcag aaguaagaag cuacuguuac gaagcaucaa uaucagauau ggcaagugac      780

ucaagauguc caacacaagg agaagcauau uuagauaaac aauccgauac ucaauaugua      840

uguaaacgaa cguuaguaga cagagguugg ggaaauggau guggccuuuu uggaaaaggu      900

ucacuaguaa cuugugccaa auuugcaugu ucaaaaaaaa ugacaggaaa aucaauacaa      960

ccagaaaauu uagaauauag aaucaugcuu ucaguccaug gaagucagca cucaggaaug     1020

aucgucaaug auacaggaca ugaaacugau gaaaauagag cuaaaguuga aauuacaccg     1080

aauuccccaa gggcugaagc aacucuuggc ggauuuggua gucugggucu agacugcgaa     1140

ccaagaacag guuuagacuu uucagauuua uacuaccuaa caaugaauaa uaaacauugg     1200

uuagugcaua aagaaugguu ucacgauauu ccauuaccau ggcacgcagg ugcagauaca     1260

gguacaccac auuggaauaa uaaagaggca cuaguagaau uuaaagaugc acacgcaaaa     1320

agacaaacag uaguaguauu agguucacaa gaaggagcag uacauacagc uuuagcagga     1380

gcauuagaag ccgaaaugga cggcgccaaa ggaagacuuu caagcgggca uuuaaaaugc     1440

agauuaaaaa uggauaaacu uaggcuaaaa ggaguaaguu auaguuuaug uacagccgcu     1500

uucacauuua cuaaaauacc agcagaaaca cuacacggua caguuacugu ugaaguucaa     1560

uacgcaggua cagauggucc cuguaaagua ccagcacaaa uggcuguaga caugcaaacg     1620

cuuaccccag uaggaagauu aauuacugca aacccaguaa uaacagaauc aacagagaac     1680

agcaaaauga uguuagaauu agauccaccu uucggugauu cauauauagu aauaggagua     1740

ggagaaaaaa aaauaacaca ucauuggcau agaaguggga guacaauugg uaaagcguuc     1800

gaagcuacug uuagaggagc aaaacgaaug gcgguauuag gagauacagc cugggauuuc     1860

ggaaguguug guggagcauu aaauaguuua ggcaaaggaa uacaucaaau auucggagcu     1920

gcauuuaaga gucuauucgg ugguaugagu ugguucucac aaauacuaau uggaacacua     1980

uuaauguggu uaggauuaaa uacaaaaaac ggaaguauau cacuaaugug uuuagcauua     2040

gguggaguuu uaauuuuucu aagcacagca guaucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9280
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 3 opt

<400>  9280
gggagaaagc uuaccaugac gaguguuggc auuguagggu uauuauuaac aacggcuaug       60

gcagcagaag uaacaagaag aggaucagca uauuauaugu auuuagauag aaacgacgca      120

ggagaagcaa uaucuuuccc aacaacacua gguaugaaua aauguuauau acaaauaaug      180

gaucuagguc auauguguga ugcuacaaug agcuaugaau gcccaaugcu agaugaagga      240

guagaaccgg augacguaga cuguuggugu aauacaacau cuacaugggu aguauacgga      300

acaugucacc auaagaaagg agaagcaaga agaucgcgua gagcagucac acuccccuca      360

cauucaacca gaaaauuaca aacuagaagu caaacuuggu uagaaucaag agaauauacu      420

aaacacuuaa uaagaauaga aaacuggaua uucagaaauc caggauuugc auuagcugcg      480

gcagcuauag cauggcucuu aggaucuucu accagucaaa aaguaauaua ccuaguaaug      540

auacucuuaa uagcaccagc auauucuaua cgaugcauug gagugaguaa cagagauuuu      600

guugaaggaa ugucaggagg aacaugggua gacguuguuc ucgaacacgg aggaugugua      660

accgugaugg cucaggauaa accaacagua gauauugaau uaguaacaac aacaguuuca      720

aauauggcag aaguccgaag uuacuguuac gaagcaucca uaucagacau ggcaucggau      780

ucaagauguc caacccaagg agaagccuau uuagauaaac agucagacac ucaauauguu      840

uguaaacgaa ccuuaguaga ucgaggcugg gguaauggau guggacuuuu cgguaaaggc      900

agucuuguaa ccugcgcaaa auucgcaugu aguaagaaaa ugacaggaaa aaguauccaa      960

ccagaaaacc uggaauauag aauuaugcua ucaguccacg gaucacaaca uucaggaaug     1020

auaguaaaug auacugguca ugaaacagau gaaaauaggg caaaaguaga aauaacucca     1080

aauucaccac gagccgaggc cacccuugga ggauuuggau cauuaggccu agauugcgaa     1140

ccuagaacag gauuagauuu uagugauuua uacuauuuaa ccaugaacaa caaacauugg     1200

uuaguucaua aagaaugguu ucaugauaua ccauuaccau ggcaugcugg agcagauaca     1260

gguacaccac auuggaacaa uaaagaagca uuaguugaau uuaaagaugc acacgcuaaa     1320

cgucaaacug uuguuguuuu aggaucacag gaaggagcug uacauacagc auuagcgggu     1380

gccuuagaag cagaaaugga cggagcaaaa ggucgacuaa guagugguca cuuaaaaugu     1440

agauuaaaaa uggacaaauu aagauuaaaa gguguuuccu auuccuuaug uacagcagcg     1500

uuuaccuuca ccaaaauacc agcagaaacc cuacacggaa caguaacagu agaaguacaa     1560

uaugcaggua cagauggacc cuguaaaguu ccugcacaaa uggcaguaga caugcaaacu     1620

uuaaccccag uaggaagauu aauaacagcc aauccaguga uuacagaauc cacagaaaac     1680

ucgaaaauga uguuagaauu ggauccacca uucggugaua guuacauagu uauaggagua     1740

ggagaaaaga aaauuacaca ccauuggcac agaagcggaa gcacaauagg aaaagcauuc     1800

gaagcaacag uaagaggagc aaaaagaaug gcugucuuag gagauacagc augggauuuc     1860

gguucuguug gcggagcuuu gaacaguuug ggcaaaggaa uacaucaaau auuuggggca     1920

gcauuuaaga gcuuauuugg agguauguca ugguucucac aaauauuaau aggaacauua     1980

cuaauguggc uaggauuaaa uacgaaaaau ggcagcauuu cacuaaugug ucuagcauua     2040

gguggagucu uaauauuucu aucaacagca guaucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9281
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 3 opt

<400>  9281
gggagaaagc uuaccaugac aucaguggga auugugggau uauuauuaac aaccgcuaug       60

gcagcagaag ucacuagaag aggaucugca uauuacaugu aucuagauag aaacgaugca      120

ggagaagcaa uaaguuuccc aacaacauua gguaugaaua aauguuacau acagauaaug      180

gaucuagguc auaugugcga ugcaacuaug aguuaugaau gcccaauguu agaugaagga      240

guagaaccag augauguaga uuguuggugu aacacaacau caacaugggu agucuacgga      300

acaugucauc auaagaaagg ugaagcuaga agaagccgca gggcaguaac uuuaccgucg      360

cacucaacaa gaaaacuuca gacaagaagu caaacuuggu uagaaaguag agaauauaca      420

aaacauuuaa uccguguaga aaauuggaua uuaaggaacc caggauucgc auuagcagca      480

gcggcaauag ccugguuacu agguucaagc acgucacaaa aaguaauaua cuuagucaug      540

auacuguuaa uugcaccagc auauagcauu cguuguauug gcguaucuaa cagagauuuc      600

guugaaggaa ugucgggagg aacauggguu gauguaguuc uagaacaugg cgguugugua      660

acaguaaugg cccaagauaa accaacagua gauaucgaac ucguaacuac aacgguaucc      720

aauauggcag aaguucgaag uuauuguuac gaagcaagua uaagugacau ggccuccgau      780

ucaagauguc cuacgcaggg ugaggcuuau cuagacaaac aaucggacac acaauaugua      840

uguaaaagaa cauuaguaga uagaggaugg ggaaauggau guggacuauu uggaaaagga      900

aguuuaguaa ccugugcaaa auuugcuugc aguaagaaaa ugacagguaa aagcauacaa      960

ccagaaaauc uugaauauag aauaaugcug agcguacacg gcagccaaca cucuggaaug     1020

auuguaaacg auacugggca ugaaacagau gaaaaucgag caaaaguaga aauaacacca     1080

aacagcccaa gggcugaagc aacccugggu ggauuuggaa guuugggucu agauugcgaa     1140

ccaagaaccg gucuagauuu cucagauuua uauuauuuaa cgaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ccaugauaua ccauuaccau ggcaugcagg agcugauaca     1260

ggaacaccac auuggaauaa caaagaagca uuaguagagu ucaaagaugc acacgcaaaa     1320

agacaaacug uaguaguacu aggaagucaa gaaggagcag uccacacagc auuagcagga     1380

gcucuagaag cagaaaugga uggugcaaaa ggccgacuau cuucuggaca uuuaaaaugu     1440

agauuaaaaa uggacaaacu aagauuaaaa ggagugucau acucauuaug cacagcagca     1500

uuuacauuua caaaaauucc agcagaaaca cuacauggaa caguaacugu agaaguccaa     1560

uaugccggaa cagacggacc uuguaaagua ccagcacaaa uggccguaga uaugcaaaca     1620

uuaacaccug uagguagacu aauuacagcc aauccaguaa uaacugaauc cacugaaaau     1680

agcaaaauga ugcuagaacu ggauccucca uuuggagauu cauauauagu cauagggaua     1740

ggagagaaaa agauaacaca ccacuggcau agaucuggaa guacuauagg uaaagcauuc     1800

gaagcaacag uaagaggugc aaaaaggaug gcaguauuag gagacacagc cugggauuuc     1860

ggaucaguag gaggugcauu aaauucauua ggaaaaggua uccaccaaau uuucggagca     1920

gcuuuuaaau cuuuauucgg uggaaugagu ugguuuucac aaauauuaau uggaacuuua     1980

cuaauguggc uaggauuaaa uaccaaaaau ggaucaauaa gucuuaugug ucuagcauua     2040

gguggaguac uuauauuucu aaguacagca guaucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9282
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 3 opt

<400>  9282
gggagaaagc uuaccaugac uucaguagga aucgucggac uuuuguuaac aacggccaug       60

gcugcagagg uaacaagaag aggaucagcu uauuacaugu auuuagauag gaaugaugcu      120

ggagaagcca uaaguuuccc aacuacacua ggaaugaaua aauguuauau ucaaauaaug      180

gauuuagguc auauguguga cgccacuaug aguuacgagu guccaauguu ggaugaaggc      240

guagaaccag acgauguuga cuguuggugc aacaccacau caacaugggu aguauauggu      300

acaugucauc auaagaaagg agaagcaaga agaucaagaa gggcaguaac acucccuuca      360

cauucaacaa gaaaauuaca aacgagaagc cagacauggc uagagucaag agaauauacu      420

aaacacuuaa uaagaguaga aaauuggaua uucagaaauc caggguuugc auuagcagca      480

gcagcaauag cuugguuacu aggaaguagu acgucacaaa aaguaauaua ucuuguaaug      540

auuuuauuaa uugcaccagc uuauagcauu agauguauag gcguuuccaa ccgugauuuu      600

guugaaggaa ugucuggagg aacauggguu gauguagugc uagaacacgg aggaugugua      660

acaguuaugg cacaagauaa accaacagug gacauagagc uaguaucaac uacugucagc      720

aauauggcgg aaguaagauc auacuguuac gaagccacaa uaagugauau agcaucagac      780

ucaagaugcc caacacaagg agaagcauau cuagacaaac aaucagacac ucaauaugua      840

uguaaaagaa cauugguaga uagaggaugg ggaaauggau gcggauuauu uggaaaaggc      900

aguuuaguua caugugcaaa auuugccugc aguaagaaaa ugacuggaaa aucaauacaa      960

ccugaaaauu uagaauauag aauaauguua ucaguacaug ggucucagca cucaggaaug     1020

auagucaaug acacaggaca cgaaacugau gaaaauagag cuaaaguuga aauaacacca     1080

aauagcccac gagcagaagc aacauuagga ggcuuuggaa guuuaggacu agauugugaa     1140

ccaagaacag gccuagacuu uucagaucua uauuauuuaa caaugaacaa caaacauugg     1200

cuaguacaca aagaaugguu ccacgauaua ccauugccau ggcacgcagg agcagacaca     1260

ggaacuccac auuggaauaa uaaagaagcu cuuguagaau uuaaagaugc acaugcaaaa     1320

agacaaacag ccguaguacu agguucacaa gaaggagcug uacacacagc acuagcugga     1380

gcauuagagg cagaaaugga uggagcuaaa ggaagauugu cgagugggca ucuaaaaugc     1440

agauuaaaaa uggacaaauu acgauuaaaa ggaguaucuu auucacuaug caccgccgca     1500

uuuacauuua caaaaauacc ggcagaaacc uuacauggaa ccguaacagu ugaaguacaa     1560

uaugcaggaa cagauggucc auguaaagua ccagcucaaa uggcaguuga uaugcaaaca     1620

uuaacaccag ucggaagacu uauaacagca aauccaguaa uaacagaauc cacagaaaau     1680

agcaaaauga ugcuggaacu agauccucca uuuggggacu cauacaucgu aauaggagua     1740

ggagaaaaga aaauaacgca ccauuggcau agaaguggua guacaauagg aaaagcauuc     1800

gaagcuacag uaagaggagc gaaaagaaug gcaguauuag gugacacugc uugggauuuu     1860

ggaucagucg gcggggcauu aaauucauua gguaaaggaa uacaucagau uuuuggagca     1920

gccuuuaaau cauuauuugg ugggaugagu ugguuuucac aaauauuaau aggaaccuua     1980

cuuauguggu uaggcuuaaa uacuaaaaau gguagcauau cacuuaugug cuuagcauua     2040

gguggagucu uaauauuuuu aucuacagca gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9283
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 3 opt

<400>  9283
gggagaaagc uuaccaugac aagcguaggu auaguugguu uacuuuuaac aacagcaaug       60

gcagcagaag uaacuagaag aggaagugca uauuauaugu accuagaucg uaacgacgcg      120

ggugaagcaa ucagcuuucc caccacuuua ggaaugaaua aauguuacau acaaauaaug      180

gacuuaggac auauguguga ugcaacaaug aguuaugaau guccaaugcu agaugaggga      240

guugaaccag augaugucga cuguuggugu aauacaacaa guacuugggu uguauauggg      300

acuugucauc auaagaaagg ugaagcuagg agaucaagac gcgcagugac acuaccuagu      360

cauagcacua gaaaacucca aacacgauca caaacauggu uagaaucacg cgaauacaca      420

aaacacuuga uacguguaga aaacuggaua uuucguaacc cagguuucgc acuggcagca      480

gcagcaauag cauggcuauu aggaaguagc acuucacaaa aagucauaua ucuagucaug      540

aucuuauuaa uagcaccagc auauaguaua agaugcauag ggguaucaaa cagagauuuc      600

guagaaggaa ugaguggagg aacaugggua gacguaguac uggaacacgg aggaugcgua      660

acuguaaugg cacaagacaa accuacagua gauauagagu uaguaacgac uacaguuagu      720

aacauggcag aaguuagaag uuauuguuau gaagcaagua uaagugauau ggcaagcgau      780

aguagaugcc caacccaagg agaagcauau uuagauaaac agagcgacac ucaauauguu      840

uguaaaagaa cuuuaguaga caggggaugg gggaacggau guggacuauu uggaaaagga      900

uccuugguua caugugcaaa auuugcaugu ucaaagaaaa ugacaggaaa aucaauccag      960

ccugaaaacc uugaauacag aaucaugcua agcguacaug ggucacaaca cucaggaaug     1020

auaguaaacg auacgggaca ugaaacagau gaaaacagag caaaagugga aauaacccca     1080

aauagcccac gagcagaagc aacucuugga gguuuuggau cucuaggauu agauugugaa     1140

ccccgaacug gucuagauuu uagugacuua uauuaucuca cuaugaauaa caaacacugg     1200

uuaguacaua aagaaugguu ccaugauaua ccacuacccu ggcaugcugg agcagacacg     1260

ggaacaccac auuggaauaa uaaagaagcu cuaguagaau uuaaagaugc acacgcaaaa     1320

agacagacag uagugguauu aggaucacaa gaaggugcag uacauacagc acuugcagga     1380

gcccuagaag cagaaaugga cggagcaaaa ggccgacuaa guaguggaca ucuaaaaugu     1440

agauuaaaaa uggacaaauu aagacuaaaa ggaguaucuu auucauuaug uacagcggca     1500

uuuacauuua cuaaaauacc cgcugaaaca cuacacggaa ccguaacagu agaacuacaa     1560

uaugcaggua cagauggucc auguaaagua ccugcccaaa uggcuguaga uaugcaaacg     1620

uuaacaccag uuggaagguu aauaaccgca aacccaguaa uaacagaauc aacggaaaac     1680

agcaaaauga uguuagaauu agacccacca uuuggugacu cauauauagu aaucggagua     1740

ggagaaaaga aaauaacaca ucacuggcau agaaguggaa guacuauagg aaaagcauuu     1800

gaagcaacag uaagaggagc aaaaagaaug gccguauuag gagauacugc uugggauuuu     1860

gguucaguag guggagcacu aaacagucua ggaaaaggaa uacaccaaau auuuggugca     1920

gcauuuaaaa gucuauucgg cggaaugucg ugguuuucac aaauacuaau agguacacua     1980

uuaauguggc uuggacuuaa uacuaaaaau ggaagcauca gcuuaaugug uuuagcauua     2040

ggaggcgucc uaauauucuu aaguacagca guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9284
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 3 opt

<400>  9284
gggagaaagc uuaccaugac aagcguaggc auaguaggau uacuauuaac aacagcaaug       60

gcugcagaag uaacaaggcg aggaagugca uauuauaugu auuuagauag aaaugacgca      120

ggcgaagcaa uaaguuuucc aacaacauua ggaaugaaca aauguuacau acaaauaaug      180

gauuuaggac auauguguga cgcaacaaug ucauaugaau guccaauguu agaugaagga      240

guggaaccag augauguaga cuguuggugc aauacuacaa guacgugggu aguauacgga      300

acgugccauc auaaaaaagg agaagcccga agaagccgaa gagcuguaac ucuacccagc      360

cauucuaccc gcaaauuaca aaccagauca caaaccuggu uagaaucaag agaauauaca      420

aaacacuuaa uuagaguuga gaauuggauc uuuagaaauc ccgguuuugc auuagcagca      480

gcggcaaucg cauggcuauu aggaucuuca acaucacaaa aaguaauaua cuuaguaaug      540

auauuauuaa uagcuccagc auacaguaua agauguauag gaguaucaaa uagagauuuc      600

guugaaggaa ugucuggagg aacaugggua gauguaguac uagaacacgg cggauguguu      660

acaguaaugg cacaagauaa accuacagua gauauagagc ucgucacaac uaccguuuca      720

aauauggcag aaguaagguc auauugcuac gaagcaagca ucagugauau ggcaagcgau      780

ucaagauguc caacucaagg agaagcauau cuagacaaac aaucagacac acaauaugua      840

ugcaaaagaa cacuuguaga caggggaugg gguaaugggu gcggccuauu cgguaaaggu      900

ucgcuaguaa caugcgcgaa auuugcaugc aguaaaaaaa ugacagguaa aaguauucaa      960

ccagaaaacu uagaauauag gauaaugcua ucuguucaug gaucacaaca cagcggaaug     1020

auaguaaaug auacuggaca cgaaacagau gaaaaccgag caaaaguaga aauaacacca     1080

aauagcccaa gagcugaagc aacucuaggu ggauuugguu cacuagguuu ggacugugaa     1140

ccaagaacag gacuggauuu uucagaucua uauuauuuga ccaugaacaa caaacauugg     1200

cuuguacaua aagaaugguu ccaugauauc ccauuaccau ggcaugcagg cgcggauaca     1260

ggaacaccac auuggaauaa caaagaagca cuaguggagu ucaaagacgc acacgcaaaa     1320

agacagacag uaguaguacu aggaucacaa gaaggagcag uucauacagc uuuagccggc     1380

gcuuuggaag cagaaaugga uggagcaaaa gguagacuua guuccggcca ucuuaaaugu     1440

cgacugaaaa uggauaaacu acgacuaaaa ggagugucau auucacucug uacagcagca     1500

uuuacuuuca cuaaaauacc agcagaaaca uuacauggaa cuguaacagu agagguacaa     1560

uaugcuggca cagacggccc auguaaagua ccagcacaaa uggcugucga uaugcagacc     1620

uuaacuccug uugguagacu aauaaccgca aauccaguaa uuacagaaag uauagaaaau     1680

agcaaaauga ugcuugaacu agauccacca uuuggugauu cguauauagu uauagggguu     1740

ggagaaaaaa aaauaacuca ucauuggcau agaucaggau caacuauagg aaaagcauuu     1800

gaagcuacag uaagaggagc aaaaaggaug gcaguuuuag gugacacagc augggauuuu     1860

gguucuguag gaggagcauu aaacucccua gguaaaggaa uacaucaaau auuuggugca     1920

gccuuuaaaa gccuauuugg agguaugucu ugguuuucac aaauauuaau aggaacacuu     1980

cuaauguggc uaggacuaaa cacuaagaau ggaaguauca gcuuaaugug uuuagcacuc     2040

ggaggaguac uaauauuuuu aucaacagca guguccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9285
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 3 opt

<400>  9285
gggagaaagc uuaccaugac aucaguugga auaguagguc uauuacuaac aacagcaaug       60

gcugcagaag uaacucggcg uggaagcgcu uauuacaugu acuuagacag aaaugaugca      120

ggcgaagcaa uaucauuucc aacaacacua ggcaugaaua aaugcuacau acaaauaaug      180

gauuuagggc auauguguga ugcaacuaug ucuuacgaau guccaauguu agaugaaggc      240

guagaaccag augauguaga uuguuggugc aauacaacuu caacaugggu aguauacggu      300

acaugucauc acaagaaagg agaagcuaga agaucaagga gagcgguaac acuaccuagu      360

cauaguacaa gaaaacuaca aacucgauca caaaccuggu uagaaucacg ugaauacaca      420

aaacauuuaa uaaggguaga aaauuggaua uucagaaauc cagguuuugc acuggcagca      480

gcugcaauag cauggcuuuu aggaucaagc acaagucaaa aagucauaua ccuggugaug      540

auauuauuaa uagcuccagc uuauaguaua cgauguauag gaguaaguaa uagagauuuc      600

guagaaggaa ugucaggagg uacauggguc gaugucguuc uagaacaugg aggauguguc      660

acgguaaugg cacaagacaa accaacagua gauauugaau ugguaacuac aacuguaagc      720

aacauggcag aaguaagauc auauuguuac gaagcaucua uaagugacau ggcaucagau      780

ucacgaugcc caacacaagg ugaagcauau uuagauaaac aaucugacac ucaauaugua      840

uguaaaagaa cucuaguaga ucgaggaugg ggaaaugguu gugggcuauu uggaaaagga      900

agccuaguaa caugcgcaaa auuugccugu ucaaagaaaa ugacaggaaa aucaauccaa      960

ccagaaaacc ucgaauauag aauaaugcua ucaguacacg gaucacaaca cagcggaaug     1020

auaguaaaug acacaggaca ugaaacagac gaaaacagag caaaaguaga aauaacaccc     1080

aauagcccca gagcugaagc aacauuagga ggauucggaa guuuaggacu ugacugugaa     1140

ccaagaacag gauuagacuu cagcgaccua uauuauuuaa caaugaauaa caaacauugg     1200

cuaguacaca aagaaugguu ucacgauaua ccuuuaccau ggcacgcagg cgcugacacu     1260

gguacuccac acuggaauaa caaagaagca cuuguagaau uuaaagaugc gcaugcaaaa     1320

agacaaacag uagugguacu aggcucucaa gaaggugcag uacauacagc acuagcaggc     1380

gcauuagaag ccgaaaugga uggcguaaaa ggaagacuau caucggggca uuuaaaaugu     1440

cgucuaaaaa uggacaaauu aagacuaaaa ggaguaucgu auucauuaug caccgcagca     1500

uucacauuua caaaaauacc agcagaaaca uuacauggaa caguaacagu agaaguacaa     1560

uaugcaggua cagacggacc auguaaaguu ccagcacaaa uggcagugga caugcaaaca     1620

uuaacaccgg uaggaagauu aauaacugca aauccaguua ucacggaaag uacagaaaau     1680

uccaaaauga uguuagaguu agacccucca uuuggagaua guuauauagu uauugguguu     1740

ggggaaaaga aaauaacuca ucacuggcau agaucaggca guacgauagg aaaagcauuu     1800

gaagcuacag uacgaggcgc caaaagaaug gcaguauuag gagauacagc augggacuuu     1860

gguagcguag guggagcccu aaauucauug ggcaaaggua uucaucaaau auuuggagcu     1920

gcauuuaaaa gccucuucgg uggaaugagu ugguuuucuc aaauacuaau aggaacauua     1980

uuaauguggu uaggacuaaa uacuaaaaau gguucuauua gcuuuaugug uuuggcacua     2040

ggaggagucu ugauauuucu aaguaccgca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9286
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 3 opt

<400>  9286
gggagaaagc uuaccaugac aucuguuggu aucguaggau uauuacucac aacagcaaug       60

gcggccgaag uaacuagaag gggaucagca uauuauaugu aucuagauag aaacgaugca      120

ggagaagcca uaaguuuucc gacaacauua ggcaugaaca aaugcuauau acaaauaaug      180

gauuuagggc auauguguga ugcaaccaug agcuaugaau gcccaauguu agacgaagga      240

guagaaccag acgacguaga uuguuggugu aacacgacaa guacaugggu cguauacgga      300

acuugucauc auaaaaaagg agaagcuagg cguucaagaa gagcaguaac auuaccauca      360

cauucaacac gaaaacuuca aacaagauca caaacauggc uagaaucaag agaauauacu      420

aaacauuuaa uaaggguuga aaacuggaua uuuagaaacc caggauuugc ccuagcugca      480

gcagcaaucg cauggcuacu aggaucaagu acaagccaaa aaguaauaua ucuaguaaug      540

auacuauuaa uagcuccagc auacucgauu agaugcauag guguaucaaa uagagacuuc      600

guagaaggaa uguccggagg aacaugggua gacguagugu uagaacaugg aggauguguu      660

acaguaaugg cacaggacaa accaaccguu gacauagaau uaguuacaac aacaguuagu      720

aacauggcug agguacguuc auauuguuau gaagcaucaa uaucagacau ggcaucagau      780

ucacgauguc ccacucaagg ugaagcauac uuagauaaac aaagcgacac acaauacguu      840

uguaaacgaa cacuaguaga uagaggaugg gggaauggau guggccuauu uggaaaagga      900

agccuaguaa caugugcuaa auuugcaugu aguaaaaaaa ugacaggaaa aagcauucaa      960

ccggaaaacu uagaauauag aauuauguua ucaguacaug guucacaaca uucagggaug     1020

auaguaaaug auaccggaca ugaaacugac gaaaacagag caaaaguaga aauaacuccu     1080

aauaguccaa gagcagaagc cacauuaggg ggauuuggga gucuaggacu agauugcgaa     1140

ccuagaacag gauuagauuu uucagaucua uauuauuuaa caaugaacaa uaaacacugg     1200

cuuguucaua aagaaugguu ucacgacaua ccacuaccau ggcaugcagg agcagauaca     1260

ggaacaccac auuggaacaa uaaagaagca uuaguggaau uuaaagaugc acaugcaaaa     1320

aggcaaacug ucguaguucu agguucccaa gaaggugcug uacauacugc acuagcaggg     1380

gcacucgaag cagaaaugga cggagcuaaa ggaaaacuaa guucagggca ccuaaaaugc     1440

agauuaaaaa uggauaaauu acguuuaaaa ggaguaucau auucauugug cacagcagca     1500

uuuacauuua caaaaauucc ggccgaaacg uuacauggca ccguuacggu agaaguacaa     1560

uacgcaggaa cagaugggcc auguaaagua ccugcccaaa uggcaguuga uaugcaaaca     1620

cugacaccgg uugggagacu aauuacugcu aacccaguaa uaacugaauc aacagaaaau     1680

ucaaaaauga uguuagaauu agauccacca uucggcgaua gcuauauugu aauaggagua     1740

ggugaaaaaa aaauaacaca ccauuggcac aggagcggua guacaaucgg caaagcauuu     1800

gaagcaacug uuagaggagc uaaaagaaug gcaguguugg gcgauacagc augggauuuu     1860

gggaguguag gaggggcacu caacucauua ggaaaaggua uacaucaaau auucggagca     1920

gcuuuuaaau cauuauucgg aggaaugagc ugguuuaguc aaauacuuau aggaacacua     1980

uuaauguggu uaggauuaaa uacaaagaac ggaagcauau cauuaaugug uuuagcacuu     2040

ggaggaguuu uaauauuucu aaguacagcu gucagugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9287
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 3 opt

<400>  9287
gggagaaagc uuaccaugac aagcguagga auuguaggac uacuguuaac aacagcaaug       60

gccgccgaag uaacaagaag aggcagcgca uauuauaugu acuuagauag aaaagaugcu      120

ggagaagcaa ucuccuuucc aacaacauua gguaugaaua aauguuauau acaaauaaug      180

gaucuaggac auauguguga cgcaacaaug ucuuaugaau guccaauguu agaugaagga      240

guagaacccg augauguaga cugcuggugu aauacgacau caacuugggu gguauacgga      300

acaugccacc auaaaaaagg agaagcaagg agaucacgua gagcaguuac acuuccuucu      360

cauucaacua gaaaauuaca aacacgaagc caaacuuggu uagaaagcag agaauauaca      420

aaacauuuaa uaagaguaga aaacuggaua uucagaaauc caggauuugc acucgcagca      480

gcagcaauag cauggcuauu ggggucaagc acaucacaaa agguaauaua cuuaguuaug      540

auauuauuaa uugcaccagc cuauucuaua agauguauag gaguaucuaa cagagauuuu      600

guugaaggua ugucaggcgg aacaugggua gaugugguuc uugaacaugg uggaugugua      660

acaguaaugg cacaagauaa accaacagua gauauagaau uaguaacaac aacaguaagu      720

aacauggcag agguaagaag uuauuguuau gaagcaagua ucucagauau ggcaagcgac      780

ucuagaugcc caacacaagg agaagcauau cuagauaaac aaagugacac acaauaugua      840

uguaaaagaa cacuaguaga uagaggaugg ggaaauggau guggacuuuu uggaaaaggu      900

ucguuaguua cuugcgcaaa auuugcaugu ucaaaaaaaa ugacuggaaa aaguauacaa      960

ccagaaaacu uagaguauag aauaauguua ucaguacacg ggucucaaca uaguggaaug     1020

auuguuaaug acacuggaca ugagacagau gaaaauagag caaaaguaga aauaacucca     1080

aacucacccc gugcagaagc gacauuagga ggauuugguu cauuaggauu agauugugaa     1140

cccaggacgg gacuagauuu uagugaucua uauuaucuaa cuaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ucacgacauc ccauuaccau ggcacgcagg agcagauaca     1260

ggaacaccuc auuggaauaa uaaagaagca uuaguugaau uuaaagacgc ucaugcaaaa     1320

cgucaaaccg uaguuguacu agguagccaa gaaggagcag uacauacugc auuagcagga     1380

gcauuagaag cagaaaugga uggcgcgaaa gguagauuaa gcaguggcca uuuaaaaugu     1440

agacucaaaa uggauaaacu cagauuaaaa ggaguuucau auagucuaug uacagcagca     1500

uuuacauuua caaaaauacc agccgaaaca cuccauggaa ccguaacagu agaagugcaa     1560

uacgcaggaa cagauggacc gugcaaagua ccagcccaaa uggcaguaga uaugcaaaca     1620

uuaacaccug uaggaagauu aauaacagca aauccaguaa uuacagaguc aacagaaaac     1680

agcaaaauga uguuagaacu agauccccca uucggagaua guuauauugu aauaggugua     1740

ggugaaaaaa agaucacaca ucauuggcac agaucaggau caacaauugg gaaagcuuuc     1800

gaagcaacag ucagaggugc aaaacgaaug gcaguacuag gagauacagc gugggacuuc     1860

ggaaguguag guggagcacu aaacucacug gggaaaggaa uacaccaaau auucggagca     1920

gccuuuaaau cacuauuugg gggcaugucu ugguucucuc aaauacuaau uggaaccuua     1980

uuaauguggc uaggacuaaa cacuaaaaau ggaagcauca guuuaaugug ucuagcacuu     2040

ggagguguuu uaauauucuu aucgacagcc guuuccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9288
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 3 opt

<400>  9288
gggagaaagc uuaccaugac aaguguagga auuguaggac uauuauuaac aacagcuaug       60

gcugcagaag uaacaagaag aggcagugca uacuauaugu auuuagauag aaaugacgcu      120

ggagaagcaa uuagcuuccc aacaacauug ggaaugaaua aaugcuacau acaaauaaug      180

gauuuaggcc auauguguga ugcaacaaug aguuaugaau gccccaugcu agacgaagga      240

guugaaccag augauguaga uugcuggugu aauacaacuu caacaugggu uguuuauggu      300

acaugucacc auaaaaaagg agaagcaagg cguucaagac gagccguaac acucccuucg      360

cacucaacuc gaaaauuaca aacccgaagc caaacauggc uagaaucaag agaauauaca      420

aaacaucuga uaagagucga aaacuggaua uuuagaaauc caggauuugc acuagcagca      480

gcagcaaucg cauggcugcu ugggagcuca accucucaaa aagugaucua ucuuguaaug      540

auacuguuga uagccccagc auacucaaua agauguauag guguaagcaa uagagauuuc      600

guagaaggca ugucaggagg aacauggguu gauguaguau uagaacacgg agguugcguu      660

acagugaugg cacaagacaa accaacagua gacauagaac uaguaacaac cacuguaagu      720

aauauggcag aaguacgauc uuauuguuau gaagcuagua uuucagauau ggcaucggau      780

ucaagguguc cuacucaagg ugaggcauac cuagacaagc aaucagacac ucaauacgua      840

ugcaaaagaa cacuaguuga uagaggaugg ggaaauggau guggauuguu cggaaaaggu      900

ucauugguaa caugugcaaa auuugcgugc ucuaaaaaaa ugacagguaa aucuauacaa      960

ccggaaaacu uagaguauag aauaaugcua aguguacaug gcucucaaca uucuggaaug     1020

auuguaaaug auacaggaca ugaaacagau gaaaauagag caaaaguaga aauaacgcca     1080

aauaguccac gagcggaagc aacacuagga ggauuuggua guuuaggauu agacugugaa     1140

ccgagaacag gauuagauuu cagcgaccua uauuauuuaa cgaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ucaugacauu ccccucccau ggcacgcagg ggcagacaca     1260

gggacaccac auuggaacaa uaaagaagca cuaguagaau uuaaagaagc acacgcaaaa     1320

agacaaacag uuguaguacu aggaagucaa gaaggagcag uacauacagc acuagcagga     1380

gcuuuagaag cagaaaugga uggagcaaag ggaagacuau cuucuggaca cuuaaaaugu     1440

agauuaaaaa uggauaaacu gagacuaaaa gguguaucau auucguuaug uacagcagcc     1500

uucacauuua cuaaaauacc agcagaaacu cuacauggaa cgguuacagu agaaguacaa     1560

uaugccggaa ccgauggccc gugcaaagua ccagcacaaa uggcagugga uaugcaaaca     1620

uuaaccccag uaggaagauu aauaacagca aauccaguaa uaacagaauc aacagaaaau     1680

agcaaaauga uguuagaauu agauccaccu uuuggagaua gcuacauagu aauaggaguc     1740

ggagaaaaaa aaauaacuca ccauuggcac agaagcggau caacaauagg aaaagcauuu     1800

gaggccacag uuagaggagc aaaaagaaug gcaguacuag gagacacugc cugggauuuu     1860

ggaagcguug gaggggcauu aaauagucuu gguaaaggga uucaucaaau auucggggca     1920

gcauuuaaau cacuauuugg aggaaugagu ugguucucac aaauauugau aggaacauug     1980

cuaauguggu uaggacuaaa uacaaaaaau ggcagcauaa gccucaugug ucuugcucua     2040

ggaggaguuc uaauauuuuu aaguacugca guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9289
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 3 opt

<400>  9289
gggagaaagc uuaccaugac aaguguugga auaguaggau uacuacuaac uacugcaaug       60

gcagcagaag uaacuagaag aggcucagcu uauuacaugu auuuagacag gaacgaugcc      120

ggcgaagcga uaaguuuucc uaccacacua ggaaugaaca aauguuacau acaaauaaug      180

gauuugggac auauguguga ugcuacgaug agcuaugagu guccaaugcu agacgaagga      240

guugaaccug augacguaga uuguuggugu aacacaacaa gcacaugggu uguauaugga      300

acaugucauc acaaaaaagg agaagcuaga agaucaagaa gagcagucac auuaccaagu      360

cauagcacaa gaaaacuaca aacaagauca caaacauggu uagaaucaag agaauauaca      420

aaacaccuua uaagaguuga aaauuggauu uuuagaaauc caggauuugc uuuagcagcu      480

gcagcaauag caugguuacu cgguucuuca acaagccaaa aaguaaucua ucuaguaaug      540

auauugcuaa uugccccagc guacucuauu cgauguauug gcguaucaaa cagagauuuc      600

gucgaaggaa ugucaggagg cacaugggua gacguuguac uagaacacgg agggugcgua      660

acaguaaugg cacaggacaa acccacaguu gauauagaau uaguaaccac aacgguauca      720

aacauggcgg aaguuagauc uuauuguuau gaggcaagua uaagugauau ggcaucagau      780

aguagauguc caacccaagg ugaagcauac uuagauaaac aaucagacac acaauacgua      840

uguaaaagga cacugguaga cagaggaugg ggaaauggau guggauuauu ugguaaagga      900

aguuuaguaa caugugcaaa auuugcaugu ucaaaaaaaa ugacgggaaa aaguauacaa      960

ccagaaaauu uagaauauag aauuauguua aguguucaug gaagccaaca uagcgguaug     1020

auagugaacg acacagguca cgaaacugau gaaaacaggg cgaaaguaga aauaacacca     1080

aauaguccgc gcgcagaagc agcuuuagga ggauucggau cacuggguuu agauugcgaa     1140

ccaagaacag guuuagauuu cagugacuug uacuaccuaa caaugaauaa uaaacauugg     1200

uuagugcaca aagagugguu ucaugauaua ccauugccau ggcaugccgg agcagauaca     1260

ggcacgccac auuggaauaa uaaagaggcg uuaguugagu ucaaagaugc acaugccaaa     1320

cgacaaacag uaguaguauu aggaucacaa gaaggggcag uucacacagc auuagcagga     1380

gcauuagaag cagaaaugga cggagcuaaa ggaagauuaa gcucaggaca ccuaaaaugu     1440

agauuaaaaa uggauaaguu gcgauuaaaa gguguaucuu acucacuuug uacagcagcu     1500

uucacauuua caaaaauacc cgccgaaaca cuacacggua caguaacagu agagguacaa     1560

uaugccggaa cagauggacc auguaaaguu ccagcacaga uggcaguaga uaugcaaaca     1620

uuaacaccug ucggaagauu aauaaccgca aauccaguga uaaccgaauc uacugaaaau     1680

ucaaaaauga uguuagaauu agacccucca uuuggagauu cauacauagu uauaggagua     1740

ggagagaaaa aaauuacaca ucauuggcau agauccgguu caacuauagg aaaagcauuu     1800

gaagcuacag uaagaggcgc aaaacguaug gcaguauuag gagacacggc augggauuuc     1860

ggaucaguag gaggagcauu aaacucauua ggaaaaggaa uacaccaaau uuucggugcg     1920

gccuuuaaau cauuauuugg uggaaugagc ugguuuucgc aaacauuaau aggcacuuua     1980

cucauguggu ugggacuuaa uacaaaaaau ggaagcauau cacucaugug uuuagcacug     2040

ggaggaguac uuauauuuuu auccacugca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9290
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 3 opt

<400>  9290
gggagaaagc uuaccaugac aaguguaggu auuguaggac uuuuauuaac uacagcaaug       60

gcagcagagg uuacuagaag aggaucugcg uauuauaugu aucuagacag aaaugacgca      120

ggagaagcaa uaucuuuucc uacgacauua ggaaugaaua aaugcuauau acaaauaaug      180

gauuuaggcc acaugugcga ugcaaccaug ucauaugaau guccaauguu agaugaagga      240

guugaaccag augauguaga uugcuggugu aauacaacau caaccugggu aguauaugga      300

accugucacc acaaaaaagg cgaagcaaga agaaguagaa gagcaguaac auuaccauca      360

cauagcacac gaaaacuuca aacacgaagc caaacauggc uugaaaguag agaauauacu      420

aaacaucuaa uaagaguaga aaacuggaua uucagaaauc caggcuuugc auuagcagca      480

gcugccauug cauggcuauu aggcaguuca acaucacaaa aaguaauuua uuuaguaaug      540

auucuauuaa uugcaccugc auauaguaua aggugcauag gaguuuccaa uagagauuuu      600

guagaaggaa ugucaggagg uacaugggua gauguaguuc uagaacaugg ugguugugua      660

acaguaaugg cucaggauaa accaacagua gauauagaac uaguaacgac aacaguaagc      720

aacauggcag aaguuagaag cuauugcuac gaagcaagua uaucagauau ggcaagcgau      780

ucacgauguc caacacaagg agaagcuuac cuugacaaac aaucugauac ucaauaugua      840

ugcaaaagga cguuaguaga ucgugguugg ggaaaugguu guggcuuauu cggaaaagga      900

agucuaguua caugcgccaa auuugcaugu aguaaaaaga ugacagguaa aucaauacaa      960

ccagaaaauu uagaauaucg aaucauguua ucaguacaug guucacaaca uucugguaug     1020

auuguaaaug acacaggaca ugaaacagac gaaaauagag caaaaguaga aaucacacca     1080

aacucuccaa gagcugaagc aacuuuagga ggguucggcu cguuaggauu agauugugaa     1140

ccaagaacgg gacuagacuu uucagaucua uauuauuuga caaugaauaa uaaacacugg     1200

cuaguucaua aagaaugguu ucaugauaua ccacuaccau ggcaugccgg agcagacacu     1260

ggaacccccc auuggaauaa caaagaagca cuaguagaau uuaaagaugc ucacgcgaaa     1320

cgacaaacug uaguaguacu uggaucacaa gaaggagcag uacacacagc auuagcagga     1380

gcuuuagaag cugagaugga cggcgcuaaa ggacgacuaa guucaggcca uuuaaaaugc     1440

agauuaaaaa uggauaaacu aagauuaaaa ggaguuucau auucacuaug uacagcagcu     1500

uuuacauuua ccaaaauacc ugcagaaaca uugcauggaa caguuacagu agaaguacaa     1560

uacgcuggag cugauggacc uuguaaagua cccgcucaaa uggcaguaga uaugcagaca     1620

uuaacaccag ucggaagauu aauuacagcc aauccaguaa uuacagaauc aacggaaaau     1680

ucaaaaauga uguuagagcu agacccgcca uuuggagauu cauauauugu gaucggugua     1740

ggcgaaaaaa aaauuacaca ccauuggcau agaucagguu cuacaauagg caaagcguuu     1800

gaagcaacug ugaggggcgc aaaaagaaug gcaguauuag gagauacugc augggauuuc     1860

ggaaguguag gaggagcacu aaacucccug ggaaaaggua uccaucaaau auuuggagcg     1920

gcguucaaau cauuauucgg uggaauguca ugguucucac aaauccucau aggaacuuua     1980

uuaauguggu uaggacuuaa uacaaaaaau ggcaguauau cauuaaugug ccuagcacua     2040

ggaggagucu uaauauuuuu aaguacugca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9291
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 3 opt

<400>  9291
gggagaaagc uuaccaugac aucaguggga auaguagguu uacuacuaac aacagcaaug       60

gcagcagaag uaacaagaag aggaucugca uauuauaugu auuuggauag aaacgaugca      120

ggggaagcaa ucaguuuucc uacaacguug ggaaugaaua aauguuauau acaaauaaug      180

gaccuaggac auauguguga ugcuacaaug ucguaugaau gcccgaugcu ggaugaaggg      240

guagaaccag augauguaga cuguuggugu aauacaacca guacaugggu gguauaugga      300

acaugucauc auaaaaaagg ugaggcucga cgaaguagga gagccguaac auuaccuuca      360

cauucaacaa gaaaauuaca aacaagguca caaacauggu uagaaaguag agaauauaca      420

aaacaucuaa uuagaguuga aaacuggaua uuuagaaacc caggguucgc uuuggcagca      480

gcagcaauag caugguuauu aggaagcuca acaucucaaa aaguuauaua ucuagucaug      540

auauuacuua uagcauuagc uuauuccaua cgauguaucg gaguaucaaa uagagauuuc      600

guagaaggaa ugucaggagg aacauggguu gacguagugu uagaacaugg uggcugugua      660

acaguaaugg cacaagauaa gccaacagua gacauugaau uaguaacuac aacaguaagu      720

aauauggcag aaguaagauc uuauuguuac gaagcaagua ucagcgauau ggccuccgau      780

ucaagaugcc cgacacaagg ugaagcauau uuagauaaac aaagcgauac gcaauacgua      840

uguaaacgua ccuugguaga cagagguugg gguaauggau gcggauuauu uggaaaaggc      900

ucacuuguua cgugugcaaa auucgcaugc ucaaaaaaaa ugacuggaaa auccauacaa      960

ccagaaaauc uagaauauag aaucaugcua uccguacaug gaucccaaca uucagguaug     1020

aucguaaaug auacagggca ugaaacagau gaaaauagag cgaaaguaga aauaacccca     1080

aauaguccaa gagcugaggc aacuuuagga ggauuuggau cacuaggcuu agacugcgaa     1140

ccgaggacag guuuagauuu uagugaucua uauuaucuua cuaugaauaa uaaacauugg     1200

uugguacaua aagaaugguu ucacgauaua ccauuaccau ggcaugcagg agcugauaca     1260

ggaacaccac auuggaauaa uaaagaagcc uugguugaau uuaaagacgc acaugcaaaa     1320

cgacaaacag uagugguauu aggaagucaa gaaggggccg ugcacacagc auuagcugga     1380

gcucuagaag cagaaaugga uggagcaaaa ggaagacuau caucaggaca ccuaaaaugc     1440

cgguuaaaaa uggacaaacu aagacuaaaa ggggugaguu acucauugug uacugcagca     1500

uuuacauuca caaagauacc agcagaaacc uuacauggua cugucacagu agaaguacaa     1560

uaugcaggaa cagauggacc auguaaagua ccugcacaaa uggcaguaga caugcagaca     1620

uuaacaccag uagguagacu aauaacugca aaucccguua uaaccgaauc aaccgaaaau     1680

aguaaaauga uguuagaauu agauccaccu uuuggggauu cauauauagu cauaggagua     1740

ggagaaaaaa aaaucacuca ucauuggcac agaagcggau caacaaucgg gaaagcauuc     1800

gaagccacag uuagaggagc aaaaagaaug gcagucuuag gagauacggc cugggauuuu     1860

ggaaguguag ggggggcauu aaauucauug ggaaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaaa guuuguucgg uggaauguca ugguuuucuc aaauacuaau aggaacucua     1980

uuaauguggc uaggucuuaa uacaaaaaau gggaguauau cauuaaugug ccuagcauua     2040

gguggagucu ugauauuccu aagcacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9292
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 3 opt

<400>  9292
gggagaaagc uuaccaugac aaguguagga auaguuggau uacucuuaac aacagcaaug       60

gcagcagaag uaacaagaag ggguucagca uauuauaugu aucuagaucg aaaugaugca      120

ggagaagcaa uaaguuuucc cacaacuuug ggaaugaaca aauguuauau acaaauaaug      180

gacuugggac auauguguga ugcuacuaug uccuaugaau guccaauguu agacgaagga      240

guagaacccg acgauguuga uugcuggugc aacacaacaa guacuugggu uguauacgga      300

acaugccauc acaagaaagg agaagcaaga agaucaagac gagcaguaac uuuaccaagu      360

cauaguacaa gaaaauuaca aacacgauca cagacauggc ucgaaucaag ggaauauaca      420

aaacaucuaa uuagaguaga aaauuggaua uucagaaauc caggauuugc auuagcagcu      480

gcagcaaucg cauggcuuuu agguucaucg acuucacaaa aaguuauaua uuuaguaaug      540

auacuucuaa uagcccccgc auauucaauu cggugcauug gagucucgaa ucgagacuuu      600

guggaaggaa ugucaggugg aacaugggua gauguagucu uagaacaugg aggaugcguu      660

acuguaaugg cucaagauaa accuacagua gauauagagc uaguaacaac uacaguaagu      720

aauauggcag aaguaagaag uuauuguuau gaggcaagua uaucugauau gguaucagau      780

ucaagauguc caacacaggg agaagcauac cuagauaaac agucagauac ucaauauguu      840

uguaaaagaa cauuaguaga ucgaggaugg gguaauggcu guggauuauu uggaaaagga      900

ucacuuguaa caugugcgaa auuugcaugu ucaaagaaaa ugacaggcaa aucaauucaa      960

ccagaaaauu uagaauacag aauaaugcua aguguacacg guucacaaca uuccggcaug     1020

auaguaaacg acacuggaca ugaaacugac gaaaauagag caaaaguuga aauaacacca     1080

aauaguccua gagcagaagc aacauuaggu ggauuuggau cauugggauu agauugugaa     1140

cccagaacag gucuggauuu uucagauuua uauuaccuua ccaugaacaa uaaacauugg     1200

cuagugcaua aagaaugguu ccacgacaua ccacuaccuu ggcaugcagg agcagauaca     1260

ggaacaccac auuggaauaa uaaagaggcu uuaguugaau uuaaagacgc acaugcuaaa     1320

agacaaacag uaguaguauu aggaagucaa gaaggagcag uacauacagc acuagcuggu     1380

gcauuagaag cagaaaugga cggugcaaaa gguagauuau caucaggaca cuuaaaaugu     1440

agauuaaaaa uggauaaacu acgccuaaaa gguguaucau auucucuaug uacagcagca     1500

uuuacauuca caaaaauucc ugcagaaaca uuacauggua caguaacagu agaaguacag     1560

uaugcaggaa cggauggacc cuguaaagua ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccag ucggaagauu aauuacugcc aacccaguaa uuacagaaag uacagaaaac     1680

agcaaaauga uguuagaacu agacccaccc uuuggagacu cuuauauagu aauaggugua     1740

ggagaaaaga aaauaacaca ucauuggcau cguaguggua gcacaauagg aaaagcguuc     1800

gaagcaacag uaagaggagc aaaaagaaug gcuguauuag gugauacagc uugggauuuc     1860

ggauccguag gaggagcacu caacaguuua ggaaaaggua uucaccaaau cuuuggagca     1920

gcauuuaaaa guuuauuugg aggaaugagc ugguuuaguc aaauauuaau ugguacucua     1980

uuaauguggu uaggauuaaa uacuaaaaau ggaucaauaa gucuaaugug ccuagcauua     2040

ggcgguguac uaauauuucu gucuacagcg guuucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9293
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 3 opt

<400>  9293
gggagaaagc uuaccaugac aucaguuggg auuguggguc uucuuuuaac aacagcuaug       60

gcagcagaag uaacaagaag aggaucagca uacuacaugu auuuagaucg aaaugaugcu      120

ggagaagcaa uaaguuuucc aacuacuuua ggaaugaaua aauguuauau acaaauuaug      180

gauuuaggac auauguguga ugcuacuaug agcuaugaau guccaaugcu agaugaagga      240

guagaacccg acgauguaga uugcuggugu aacacuacaa gcacgugggu uguauaugga      300

acuugccacc auaagaaagg agaagcuaga agaaguagac gagcaguaac acuaccuagu      360

cauucaacga gaaaacuaca aaccagauca caaacauggc uagaaucaag agaauauaca      420

aaacaccuca uaagaguuga aaacuggaua uuuaggaacc caggauuugc ucuagcagca      480

gcugcaaucg caugguuauu aggauccuca acaucacaaa aaguuauaua cuuaguaaug      540

auuuuauuaa uagcaccagc uuauuccauu agauguauag guauaagcaa cagggacuuu      600

guagaaggaa ugaguggagg aacuuggguc gauguagucu uagaacacgg cggaugcgua      660

acuguaaugg cacaggauaa accaacugua gauauagaau uaguaaccac aacuguaucu      720

aauauggcug aaguaagauc auacugcuau gaagcaagua uaucugauau ggcaucagac      780

ucaagaugcc caacacaagg agaagccuau uuagacaaac aaagugauac acaauacgua      840

uguaaaagga cauuaguaga uagaggaugg ggaaauggcu gcggauuauu ugguaaagga      900

agucuaguaa cuugugccaa auucgccugu ucaaagaaaa ugacaggaaa aucaauucaa      960

cccgaaaauc ucgaauaucg uauaauguug ucaguacacg gaucacaaca uaguggaaug     1020

auaguuaacg acacuggcca cgaaacggac gaaaaucgag caaaaguaga aauaacacca     1080

aauaguccua gagcagaagc gacauuagga ggauuuggca uauuaggauu agauugugaa     1140

ccaagaacug gacucgauuu uucagauuua uacuauuuga caaugaauaa uaaacacugg     1200

uuaguacaca aagaaugguu ucaugacaua ccuuuaccgu ggcaugcagg agcagacaca     1260

ggaacgccuc acuggaacaa uaaagaagca uuaguggagu ucaaagaugc acacgcaaaa     1320

agacaaacag uaguaguauu agguagucaa gaaggagcag uacauaccgc acuagcuggc     1380

gcuuuagaag cagaaaugga uggcgcaaaa ggaagauuau caagcggaca uuuaaaaugu     1440

aggcuaaaaa uggauaaacu aagauuaaaa ggaguaagcu auagcuuaug cacagcagca     1500

uucacauuua caaaaauacc agcagagaca uuacauggua caguuacugu agaaguccaa     1560

uaugcaggua cugauggacc auguaaagua ccggcacaaa uggcaguaga caugcaaaca     1620

uuaacaccug uugguagacu aaucacagca aauccuguaa uaacagaauc aacagaaaac     1680

ucgaaaauga uguuagaauu ggacccucca uuuggagacu cauauauugu cauaggagua     1740

ggagaaaaga aaauaacuca ucauuggcau agaucaggau caacaauagg aaaagcauuu     1800

gaagcaacag ugagaggagc caaaagaaug gcgguucuug gagacacagc uugggauuuu     1860

ggaucuguag gaggagcauu aaauaguuua ggaaaaggaa uacaucaaau auuuggcgcc     1920

gcuuuuaaaa gccuuuuugg aggaauguca ugguuuaguc aaauacugau agguacgcua     1980

uuaauguggc uugguuugaa uacgaaaaau ggaaguauaa guuuaaugug ucuggcacuu     2040

ggagggguau uaauauucuu aucaacagca gugucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9294
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 3 opt

<400>  9294
gggagaaagc uuaccaugac caguguaggg auaguaggau uacuauuaac uaccgcaaug       60

gccgccgaag uaacaagaag aggcagugca uauuacaugu acuuagacag gaaugacgcc      120

ggugaagcua uaaguuuucc aacaacuuua ggaaugaaua agugcuauau ucaaauaaug      180

gacuuaggac auacguguga ugcaacaaug ucauaugaau guccgauguu ggacgaagga      240

guagaaccag augauguaga uuguuggugu aacacaacga guacaugggu uguauaugga      300

acuugucauc acaagaaagg agaagcaaga agaagcagac gagcaguaac ccuucccucu      360

cacaguauaa gaaaacuaca aacaaggagc caaacauggc uagaaaguag agaauauaca      420

aaacaucuga uuagaguuga aaauuggaua uucagaaauc cuggauuugc guuagcagcg      480

gcagcaauag caugguuauu agguaguuca acaagucaaa aaguaauuua ccuaguaaug      540

auauuacuaa uugcaccagc auacucuaua aggugcaucg gaguaucaaa uagagauuuc      600

guagaaggua ugucaggagg aacaugggua gacguagugu uagaacaugg uggaugugua      660

acuguaaugg cgcaagauaa accaacagug gauauagaac ugguaacaac uacuguaagc      720

aauauggcag aaguacgauc guauuguuau gaggccagua uaucugauau ggccagugac      780

aguagaugcc caacacaagg ugaagcauac cuagacaaac aaucagacac ucaguauguu      840

uguaaacgaa cauuaguaga ucgagguugg gguaauggau guggguuauu cggaaaagga      900

aguuuaguaa cuugugcaaa auuugcaugu ucaaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaauc uagaauauag aauaauguua ucuguacaug guagccaaca cagcggaaug     1020

auuguuaacg acacaggaca ugaaacggau gaaaauagag cgaaaguuga aauaacccca     1080

aauagcccaa gagcagaagc aacuuuaggu ggauuuggau cauuagguuu agauugugaa     1140

ccaagaaccg gacuagacuu uagugaccua uauuauuuaa caaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ucacgauaua ccacucccau ggcacgccgg agcagauaca     1260

gguacaccac acuggaauaa uaaagaagca uuaguagagu ucaaagaugc gcacgcaaaa     1320

agacaaacug uugugguucu aggaagucaa gaaggcgcag uucauacugc auuagcggga     1380

gcacuagagg ccgaaaugga cggagcaaaa ggaagacuua gcucagguca ucuaaaaugc     1440

agauuaaaaa uggauaaacu ccgauuaaaa ggaguaucau acaguuuaug uacagcagca     1500

uuuacauuua caaaaauacc agcagaaacu cuacauggaa cugugacagu agaaguacaa     1560

uacgcgggua cugacggacc uuguaaagua ccagcucaaa uggccgugga uaugcaaaca     1620

uuaacaccag uuggaagauu aauuacagcu aacccaguaa uaacugaauc aacagaaaac     1680

agcaaaauga uguuagaauu agacccgcca uuuggagaca guuauauagu aauuggagua     1740

ggcgaaaaga aaauaacuca ucacuggcac cgaucaggau caacaauagg uaaagcauuu     1800

gaagcaacug uucgaggagc aaaaaggaug gcaguacuag gcgauacagc gugggauuuu     1860

ggaucuguag gaggugccuu aaauucuuua ggaaaaggua uacaccaaau uuuuggagca     1920

gcauuuaaau cacuauuugg uggaaugagc ugguucucac aaauauuaau aggaacacua     1980

uuaauguggc uuggucuaaa ugcuaaaaau gguaguauaa guuuaaugug uuuagcauua     2040

ggaggaguac uaaucuuccu aagcaccgca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9295
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 3 opt

<400>  9295
gggagaaagc uuaccaugac auccguaggu auaguaggau uacuacuaac aacagccaug       60

gccgcagaag uaacccgucg aggaucugca uacuauaugu auuuagaccg aaacgacgca      120

ggagaagcua uauccuuccc aacaacucua ggaaugaaua aauguuauau ucaaauaaug      180

gauuuaggac auaugugcga ugcaaccaug ucauaugaau guccaauguu agaugaaggg      240

guagaaccag augauguaga cugcuggugu aauacaacuu ccacaugggu uguauacgga      300

acgugucauc auaagaaagg agaagcgcaa agaaguagac gagcaguuac acuaccaagu      360

cauaguacaa gaaaauuaca aaccagaucu caaacauggc uagaaucaag ggaauacacu      420

aaacaucuaa uaagaguaga aaauuggauu uuuagaaauc cuggauuugc acuagcggca      480

gccgcaauag cauggcuauu aggaaguucu acaucacaaa aaguaauaua cuuaguaaug      540

auacuauuaa uagcaccagc uuacaguauc agaugcauag gaguuucaaa cagagauuuc      600

guagaaggca ugaguggagg aacauggguu gauguaguac uagaacaugg uggaugcgua      660

acaguaaugg cacaagacaa accaacagua gauauugaau uaguaacaac aaccguauca      720

aauauggcug aagucagaag uuacuguuau gaagcuagca uaagugauau ggcaagugau      780

ucaaggugcc caacacaagg ugaggcuuau cuagacaaac aaagugacac acaauaugua      840

uguaaaagaa cuuuaguaga ccgaggaugg ggaaauggau guggauuauu cggaaaagga      900

uccuuaguaa cuugcgcuaa auuugcgugu ucaaagaaaa ugacagguaa aaguauacaa      960

ccagaaaauu uagaauauag aauaaugcua ucaguacacg gaagccaaca uucaggcaug     1020

auaguaaacg auaccggaca ugaaaccgac gaaaacagag caaaaguuga aauuacaccc     1080

aacagccccc gagcagaagc aacccuugga gguuuuggca gcuuaggauu agauugugaa     1140

ccaagaaccg gauuagauuu uagugaccua uauuaucuaa caaugaauaa uaaacauugg     1200

uuaguacaca aagaaugguu ucacgacaua ccauuaccau ggcaugcagg agcagacacu     1260

ggaacaccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc ucaugcaaaa     1320

cgacaaacag uaguaguauu aggcagucaa gaaggggccg uacauacagc cuuagcaggg     1380

gcacucgaag cagaaaugga cggagcuaaa ggaagacuau caagugguca uuuaaaaugu     1440

cgcuuaaaga uggauaaauu gagauuaaaa ggaguaaguu auucacuaug uacagcugca     1500

uuuacauuca caaaaauacc agcagaaaca cuucauggaa ccguaacagu agaaguacaa     1560

uaugcaggga cagauggacc auguaaagua ccugcacaaa uggcaguuga uaugcaaacc     1620

uuaacaccag uaggaagacu aauaacagca aauccaguaa uaacagaauc gacagaaaau     1680

aguaaaauga uguuagaauu agacccaccc uuuggagacu cauauauagu gauaggugua     1740

ggugaaaaga aaauaacuca ccauuggcau agauccggaa guacaauagg aaaagcauuu     1800

gaagcaacag uaagaggagc gaaacguaug gcuguacuag gagauacagc augggauuuu     1860

ggaucaguag gaggagcacu aaauagccuu ggaaaaggaa uacaccagau auuuggagca     1920

gcauuuaaau cuuuauuugg aggaauguca ugguucaguc aaauauuaau agguacauua     1980

cuaauguggu uagguuuaaa cacuaaaaau ggaucuauau cuuuaaugug uuuagcauua     2040

gggggaguuu uaauauuucu aucaacagcu guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9296
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 3 opt

<400>  9296
gggagaaagc uuaccaugac uuccguaggu auaguagguu uacuauuaac aacugccaug       60

gccgcagaag uaacuagaag agguagcgca uauuauaugu acuuagaucg aaacgaugcc      120

ggagaagcaa uaucauuccc aacaacauua ggcaugaaua aauguuauau acaaauaaug      180

gauuuaggac auauguguga ugcaacaaug ucauacgaau guccgaugcu agacgaaggu      240

guagaaccag acgauguaga cuguuggugu aacacaacuu ccacaugggu uguuuaugga      300

acuugucauc auaaaaaagg agaagcaaga cguucacgua gagcuguaac ccuaccauca      360

cauucaacaa gaaaacuuca agcaagauca caaacauggc uagagucuag agaauacaca      420

aaacauuuaa uaagaguaga aaacuggaua uuuagaaauc cgggauucgc uuuagcugca      480

gcagcaaucg ccuggcuauu agguucaagc acaagucaaa aaguaauaua cuuagucaug      540

auauuauuga uagccccagc uuauucaaua agauguauag gaguuucaaa cagggauuuu      600

gucgaaggaa ugucaggagg aacaugggua gaugucguau uagaacacgg aggaugugua      660

acaguaaugg cacaagauaa accaacaguu gacaucgaau uaguuacaac gacaguaagc      720

aauauggcug aaguaagauc uuauuguuau gaagcaagca uuagugacau ggcaagugau      780

ucaaggugcc caacacaagg agaagcuuau uuagacaaac aauccgauac ucaauacgua      840

uguaaaagaa cauuagugga uagaggaugg ggcaauggau guggccuauu ugguaaagga      900

aguuuaguaa cuugugcaaa auuugcaugu aguaaaaaaa ugacagguaa gagcauacaa      960

ccagaaaauc uagaauaucg aauaauguua ucuguucaug gcagucaaca cucaggaaug     1020

auaguaaaug acacaggaca ugaaacugau gagaauagag caaagguaga aauaacacca     1080

aacagcccaa gagcugaagc aacauuagga ggguuuggau cauuaggacu cgauugugaa     1140

ccaaggacug gcuuagacuu uucagauuua uauuauuuaa caaugaauaa uaaacauugg     1200

cuaguacaca aagaaugguu ucaugauauu ccgcuaccau ggcacgcagg agcagauacg     1260

ggaacucccc auuggaauaa uaaagaagcc uuaguagagu ucaaagacgc acacgcaaaa     1320

agacaaacag ucguaguacu uggaagucaa gaaggagcag uacauacagc uuuagcaggg     1380

gcucuugaag cagagaugga cggagcaaaa ggaagacugu caagcggaca ucuaaaaugc     1440

agauuaaaaa uggauaaacu aagauuaaaa ggaguaucuu acagucuuug caccgcugcu     1500

uuuaccuuua caaaaauacc agcagaaacu uuacauggaa cuguuaccgu agaaguccag     1560

uacgcuggaa cagauggccc augcaaagua cccgcacaga uggcuguaga uaugcaaaca     1620

uuaacaccag uagguaggcu gauaacagca aaucccguaa uaacagaauc aacagaaaau     1680

ucaaaaauga ugcuagaacu ugacccaccu uuuggcgaua guuauauagu uauaggagua     1740

ggagaaaaaa aaauaacaca ucauuggcac agaucugguu ccacgaucgg aaaagccuuu     1800

gaagcaacag uaaggggagc gaaaagaaug gcuguacuag gugacacagc cugggauuuc     1860

ggaucaguug gaggugcacu aaauucauua ggaaaaggaa uacaccaaau uuuuggagca     1920

gcauuuaaau cauuauucgg cggaaugagc ugguuuucac agauuuuaau aggaacacua     1980

uuaauguggc uaggacuaaa uacaaaaaau ggaucgauaa gucuaaugug ucuagcacua     2040

ggaggaguau ugauauuucu aucaacggca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9297
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 3 opt

<400>  9297
gggagaaagc uuaccaugac aucaguaggc auaguaggac uauuacuaac aacagccaug       60

gcagcggaag uaacaaggag aggaucugca uauuacaugu acuuagaucg auccgaugca      120

ggagaagcaa uaaguuuucc aacaacuuua gguaugaaua aauguuauau acaaauaaug      180

gauuuaggac acauguguga ugcuacuaug ucuuaugaau gcccaauguu agacgaagga      240

guggaaccug augauguuga uuguuggugu aauacaacaa guacaugggu aguauauggu      300

acaugucauc auaagaaagg agaagcgagg agaucaagga gagcuguaac auuaccaagu      360

cauagcacaa gaaaauuaca aacaagaagc caaacuuggu uagaaaguag agaauacaca      420

aagcauuuaa uaagaguaga aaauuggaua uuuagaaacc caggauuugc acuagcagca      480

gcagcaauag cauggcuauu aggcaguagu accucacaaa aagucauaua uuuaguuaug      540

auacuuuuaa uagccccugc auauagcauu agauguauag gcguuagcaa ucgagauuuu      600

guagaaggua ugucaggagg uacuugggua gauguaguau uagaacacgg gggauguguc      660

accguaaugg cccaagacaa accaacugua gauauagagu uaguuacuac aacaguaagc      720

aauauggcag aagucagaag uuauugcuac gaagcaagca uaagcgauau ggcuagugau      780

agccgauguc cuacccaagg agaggcguau uuagauaaac aaucugacac gcaauaugua      840

uguaaaagaa cucuaguaga cagaggaugg ggcaacggau gcggauuauu uggaaaaggu      900

agcuuaguaa caugugcaaa auuugcaugu ucaaagaaaa ugacuggaaa aaguauucaa      960

ccagaaaacu uagaauaucg aauaaugcua ucaguacaug gaagucaaca uucaggaaug     1020

auaguaaacg acacuggaca cgagacagau gaaaaucgag cgaaaguaga aauaacacca     1080

aauucaccua gagcugaagc aacauuaggu ggauuuggua gcuuaggauu agauugugaa     1140

ccaagaaccg gucuagauuu cagcgaccua uauuauuuaa caaugaauaa uaaacacugg     1200

cuaguacaua aagaaugguu ucaugauauu ccucuaccuu ggcacgcugg agccgacacu     1260

ggaacaccuc auuggaacaa uaaagaagcg uuagucgagu ucaaagaugc ccacgcaaaa     1320

agacaaacug uaguaguauu aggaucacaa gaaggagcag uacauacagc acuagcagga     1380

gcacuagaag cagaaaugga uggagcaaag ggaagauuaa gcaguggaca ccuaaaaugu     1440

agacuaaaaa uggacaaacu aagauuaaaa ggaguuucuu auagucuaug cacggcagcu     1500

uucacauuua guaaaauucc agcugaaaca uuacauggca caguaacaga agaaguccaa     1560

uaugcaggua cugauggacc auguaaagua ccagcacaaa uggcaguaga uaugcagaca     1620

cuaacacccg uagguaggcu uauaacagcg aauccaguaa uaacugaaag uacugaaaau     1680

aguaaaauga uguuagaauu agauccccca uuuggugauu cauacauagu aauaggagua     1740

ggagaaaaga aaauuacaca ucauuggcac cguucaggau caacaaucgg aaaagccuuu     1800

gaagcaacug uuagaggagc aaaacgaaug gcaguuuuag gagauacugc augggauuuc     1860

ggaaguguag gaggggcauu gaauagcuug ggaaaaggua uucaucaaau auucggagca     1920

gcauuuaaaa gucuauuugg ugguaugagu ugguuuaguc aaauacuaau uggaacauua     1980

cuaauguggc uagguuuaaa uacgaaaaac ggaucaauca guuuaaugug ccuagcucua     2040

ggcggcgucc uaauauuuuu gaguacagca guuuccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9298
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 3 opt

<400>  9298
gggagaaagc uuaccaugac aucuguagga auaguaggac uacuacuaac uacagccaug       60

gcugcagagg uaacaagaag aggaucagca uauuauaugu auuuggauag gucagaugcc      120

ggugaagcaa uaaguuuucc uacuacauua ggaaugaaca aauguuauau acaaauaaug      180

gauuuaggac auauguguga ugcaacaaug aguuaugaau gcccgaugcu agaugaagga      240

gucgaaccag augaugucga uuguuggugc aacacaacau caacgugggu ugucuaugga      300

acaugucacc acaagaaagg ugaagcuaga agaucaagga gagcaguuac auugcccagu      360

cauaguacaa gaaaauuaca aacaagaagu caaacauggu uagaaaguag agaauauacu      420

aaacaucuaa uacgugugga aaauuggaua uuuagaaauc caggauuugc gcuagcagca      480

gcagcaauag caugguuacu agguucuagu acaagucaaa aaguaauaua ucuagugaug      540

auauuauuaa uugcacccgc auauaguaua aggugcauag gugucagcaa uagagauuuu      600

guggaaggaa ugucuggagg aaccuggguu gauguaguuu uagaacaugg gggaugcguu      660

acaguaaugg cacaggauaa acccacaguu gauaucgaau ugguuacgac aacaguuagu      720

aacauggcag aaguaagauc auacuguuau gaagcaagua uaucagauau ggcaucagau      780

ucaagauguc caacccaagg agaagcauau uuagacaaac aaagugauac ccaauacgua      840

uguaaacgaa cucuuguaga uagaggaugg ggaaacggau guggauuauu uggaaaaggu      900

agucuaguaa cuugcgcaaa auuugcuugu ucaaagaaaa ugaccggaaa aucaauacaa      960

ccagaaaacu uagaauauag aauuaugcua aguguacaug gaucucagca cagcgggaug     1020

auaguuaacg acacagguca ugaaacagac gaaaauagag caaaaguaga aauaacacca     1080

aacaguccaa gagcagaagc aacccuuggu ggauuuggga guuuaggucu ggauugcgaa     1140

ccgagaacag gauuagauuu uagugauuua uauuaucuaa caaugaauaa uaaacauugg     1200

uuaguacaua aagaaugguu ccaugauaua ccacuuccau ggcauacggg agcagacacc     1260

ggaaccccac auuggaacaa uaaagaggca uuaguugaau uuaaagacgc acacgcaaaa     1320

agacaaacag uaguaguacu gggcucccaa gaaggagcag uacauacagc acuagcgggu     1380

gcacucgaag cagaaaugga uggagcgaaa ggaagauuaa gcaguggcca ucuaaaaugu     1440

agguugaaaa uggacaaacu aagguuaaaa ggaguaaguu acagccucug uacagcugcu     1500

uucaccuuua caaaaauacc ggcagaaaca uuacacggaa caguaacagu agagguucaa     1560

uaugcaggaa cagauggacc auguaagguu ccagcacaaa uggcagucga uaugcaaaca     1620

uuaacuccag uuggaagauu aauaacagca aauccuguua uaacagaagg aacagaaaac     1680

ucaaaaauga uguuagaacu agauccacca uuuggcgaua guuauaucgu aauaggagua     1740

ggggaaaaga aaauaacuca ucacuggcau agaagcggau caaccauagg uaaagcauuu     1800

gaagcaacgg uaaggggugc aaaaagaaug gcaguauugg gagacacagc uugggacuuu     1860

ggaaguguag gagguguauu aaacagucua ggaaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaaa guuuauuugg aggaaugagc ugguuuucac aaauauuaau aggaacauua     1980

cuuauguggu ugggacuaaa uacuaaaaau ggaucgauaa gccugaugug uuuagcacua     2040

gguggaguuu uaaucuuccu aaguacagca guaagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9299
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 3 opt

<400>  9299
gggagaaagc uuaccaugac aucaguagga auaguaggau uacuacuaac cacagcaaug       60

gcagcagagg uaacaaggag aggaucagca uauuauaugu auuuagaucg aucagacgca      120

ggagaagcaa uaaguuuucc aaccacgcua ggcaugaaua aauguuacau ucaaauaaug      180

gacuuagguc auauguguga ugcaacaaug uccuaugaau guccuauguu agaugaagga      240

guugaaccug augauguuga uuguuggugu aacacaacau caacuugggu aguauauggu      300

acaugucacc auaaaaaagg agaagcuaga cgaaguagaa gagcuguaac acuaccaagc      360

cacucgacua gaaaacuaca aacaagauca caaacauggu uagaaaguag agaauacaca      420

aaacaucuua uacgaguaga gaauuggaua uuucgcaauc caggauuugc acuagcagca      480

gcagcaauag cgugguuauu agguucaucu acaagccaaa aaguaauaua uuuaguaaug      540

auauuauuaa uagcaccagc guauucaaua agauguauag gagucaguaa uagggauuuc      600

guagaaggaa ugucuggcgg aacaugggua gauguaguac uagaacaugg aggaugcgua      660

acaguaaugg cucaagauaa accaacuguu gauauagaau uaguaacaac aacaguaagc      720

aauauggcag aaguaagauc auacuguuau gaggcaagua uaagugauau ggcaucagau      780

agcagauguc ccacacaagg cgaagcauau cucgacaaac aaagcgacac gcaauaugua      840

uguaaacgaa cucuaguaga uagagguugg gguaacggcu guggacuauu uggaaaagga      900

aguuuaguaa ccugcgcuaa auucgcaugu ucaaaaaaaa ugacgggaaa aucaauacaa      960

ccagaaaacu uagaauauag aauaauguua agcguacaug gcagucaaca cucaggaaug     1020

auaguaaaug auacagguca ugagacugac gaaaaccgag ccaaaguaga aauaacucca     1080

aauucaccaa gagcugaagc aacacuugga gguuuuggca guuuagguuu agacugugaa     1140

ccaagaacag gacuagacuu uuccgaucua uauuaccuaa caaugaacaa uaaacauugg     1200

uuaguacaua aagaaugguu ucaugacaua ccauuaccuu ggcaugcagg agcagauacc     1260

ggaacaccuc acuggaauaa uaaagaagca cuaguugaau ucaaagaugc acaugcuaaa     1320

agacaaacug ucguuguacu agguagucaa gaaggagcag uucauaccgc acuagcggga     1380

gcacuugaag cagaaaugga cggggcaaaa ggaagauuaa gcucaggaca uuuaaaaugc     1440

agacuaaaaa uggauaaacu aagacuaaaa ggagucucau auaguuuaug uacagcagcg     1500

uuuacauuua caaagauucc agcagaaaca uuacauggaa caguaacugu agaaguacag     1560

uaugcaggaa cugacggacc auguaaaguu ccagcacaaa uggcuguuga uaugcaaacu     1620

uuaacuccag uaggaagauu gauuacagca aaucccguga uaacagaauc cacagaaaau     1680

ucuaaaauga uguuagaacu cgaucccccg uuuggagaua gcuauauagu aauaggagua     1740

ggagaaaaaa aaauaacaca ccauuggcac aggucagguu ccacaauagg uaaagcuuuu     1800

gaagcuacag uaagaggagc uaaaagaaug gcuguacuag gagauacagc augggauuuu     1860

ggaagcguag gcggagcacu uaauucauua ggaaaaggua uccaccaaau auucggagcc     1920

gcuuucaaau cuuuauuugg aggaaugagu ugguuuagcc aaauauuaau aggcacucua     1980

cuaauguggu uaggacuaaa cacuaaaaau ggaucuauau cauuaaugug uuuagcauua     2040

ggcggaguuc uaauuuuucu cucaacagca guaucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9300
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 3 opt

<400>  9300
gggagaaagc uuaccaugac aucaguagga auaguuggau uauuauuaac aacagcaaug       60

gcaguagaag uaacuagaag aggaaaugca uauuacaugu acuuagaucg aucagaugcc      120

ggugaagcga uuagcuuucc aacaaccaug ggaaugaaca aaugcuacau acaaauaaug      180

gaccuaggcc auaugugcga cgcuacaaug ucauacgaau guccuauguu agaugaggga      240

guagaaccug augauguuga cuguuggugu aauacaacaa guacuugggu aguauaugga      300

acaugccauc auaagaaagg agaagcaagg agaucaagac gagcaguaac acuuccaagc      360

cauucaacua gaaaacuuca aacaagaucu caaacguggc uagaaucaag agaauauaca      420

aaacacuuaa uaagaguaga aaauuggauu uuccgaaauc caggcuuugc auuagcagca      480

gcugcaauag cuugguugcu aggaaguuca acaucacaaa aaguaauuua ccucguaaug      540

auauuauuaa uagcacccgc auacucaauu cgaugcauag gaguaaguaa cagagacuuu      600

gucgaaggua ugucaggagg gacuugggua gauguaguac uagaacaugg agguugcgua      660

acaguaaugg cucaagauaa accaacagua gacauagaau uagucacuac aacgguauca      720

aacauggccg aaguacgauc auauuguuau gaagcaucaa uaucagacau ggccagugac      780

uccagauguc caacacaagg ugaagccuau cuagauaaac aaagugauac gcaauaugua      840

uguaaacgaa cacuuguaga cagagguugg ggaaacggcu gcgguuuauu ugguaaagga      900

aguuuaguaa caugugccaa auuugcaugu uccaagaaaa ugacagguaa aagcauacaa      960

cccgaaaauu uggaauaucg aauuauguua ucaguacacg gaucacaaca cucagguaug     1020

auagucaaug auacugguca cgaaacugau gaaaauagag caaaagucga aauuacaccc     1080

aauucgccua gagcugaagc aacguuagga ggauuuggcu cauuaggauu agauugcgaa     1140

ccaagaacag gucucgauuu cucagacuua uauuacuuaa ccaugaauaa uaaacauugg     1200

cucguucaca aagaaugguu ucaugauaua ccucuacccu ggcaugcagg agcagauacc     1260

gguacaccac auuggaauaa caaagaagcg cuaguagaau uuaaagacgc acaugcaaaa     1320

cgacaaacug uaguaguuuu aggaucacaa gaaggagcag uacauacugc auuagcugga     1380

gcauuagaag cagaaaugga uggagcaaaa ggaagguuaa guagugguca uuuaaaaugu     1440

agauuaaaaa uggauaaguu aagacuaaaa ggagugaguu auucacuaug cacagccgcc     1500

uuuacauuua cuaaaauucc agcagaaaca uugcauggaa cagucacagu ggaaguacaa     1560

uacgccggaa cagacggacc augcaaaguc ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaaccccag uaggaagacu aauaacagca aauccuguaa uaacugaauc aacggaaaau     1680

ucaaaaauga uguuggaauu agauccacca uuuggcgauu cauauauagu uauaggcguc     1740

ggagaaaaga aaauaaccca ucauuggcac agaucagguu caacaauagg aaaagcauuu     1800

gaggccacgg uaagaggcgc aaaaagaaug gcaguauuag gagacacagc augggauuuc     1860

ggaagcguug gaggagcacu caauagcuua gguaaaggaa uccaucaaau uuuuggagca     1920

gcguucaaau cauuauuugg uggaauguca ugguuuucac aaauauuaau aggaacuuua     1980

uuaguauggu uaggacuaaa uacuaaaaac ggaucuauaa gccuaaugug uuuagcacuc     2040

ggaggaguau uaauuuuccu aaguacagca guuagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9301
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 3 opt

<400>  9301
gggagaaagc uuaccaugac aucaguagga auaguugguc uauuauugac cacagcaaug       60

gcugccgaag ugacaagaag agguucagca uauuauaugu aucuugaucg gucugaugca      120

ggagaagcga uaaguuuucc aacaacucua ggaaugaaca aauguuauau acaaauaaug      180

gaccuagggc auauguguga cgcaacaaug aguuaugaau guccaaugcu agaugaagga      240

guagaaccag acgauguuga cuguuggugu aauaccacca guacaugggu aguauauggc      300

acaugucauc auaaaaaagg agaagcgcga cguaguagac gcgccguaac acucccauca      360

cauucaacaa gaaaacuuca aacaagaagc caaaccuggu uagaaaguag agaauacaca      420

aaacaccuua uaagaguaga aaacuggaua uuuagaaauc caggcuucgc auuagcagcu      480

gcagcaaucg caugguuacu cggaucauca acaucccaaa aaguaauaua ucuagugaug      540

auacuauuaa ucgcaccagc auacucaaua agauguauag gaguaaguaa cagagauuuu      600

guugaaggua ugaguggggg gacaugggua gauguaguac uagaacaugg cggaugcgua      660

acagucaugg cucaagauaa acccacagua gauauagaac ucguaacaac aacaguauca      720

aacauggcag aagucagauc auauuguuau gaggcaagua uaagugacau ggcaucagac      780

aguagguguc caacccaagg agaagcuuau cuagacaaac aaagugauac ccaauaugua      840

uguaaaagaa cuuuaguaga cagaggaugg ggaaauggau gcggauuauu uggaaaaggu      900

ucguuaguaa cgugcgcaaa auucgcaugu agcaaaaaaa ugacugguaa aucaauacaa      960

ccagaaaauc uagaauacag aauaaugcuc ucugugcacg guagucaaca uucuggaaug     1020

auaguaaaug auacaggaca ugaaacggau gagaacagag caaaaguaga aauaacacca     1080

aacaguccaa gagcagaagc aacacuaggg gguuucgguu cuuuaggauu agauugugaa     1140

ccaagaacag gacuagauuu uagcgaucua uauuauuuaa caaugaacaa caaacauugg     1200

uuaguacaua aagaaugguu ucaugauaua ccacucccau ggcaugcagg cgccgauaca     1260

ggcacaccac auuggaauaa caaagaagca cuaguggaau uuaaagaugc acacgccaaa     1320

agacaaacug uaguaguacu aggaucacaa gaaggagcug uacauacagc auuagcaggu     1380

gcacuagagg cagaaaugga uggagcaaaa ggaagauuau cuagcggcca ucuaaaaugc     1440

aggcuaaaaa uggauaaauu aagacuaaaa ggagugucau acucucucug uacugcagca     1500

uucacuuuua caaaaauacc cgcagaaacg uuacacggaa cuguaacagu cgaaguucaa     1560

uaugcaggaa cagauggacc auguaaagua ccagcacaaa uggcaguaga uaugcaaacu     1620

uuaacuccag uuggccgacu aauuacagca aauccaguua uuacagaguc aacagaaaau     1680

agcaaaauga ugcuagaauu agacccacca uucggagauu cauauauagu cauagggguc     1740

ggagaaaaaa aaauuacgca ucacuggcac agaagcggau caacaauagg aaaagcuuuc     1800

gaagcgacag uaagaggagc aaaaagaaug gcaguuuuag gggauacagc augggacuuu     1860

ggauccgucg guggagcacu aaauagucua ggaaaaggaa uacaucaaau auucggagcg     1920

gccuuuaaau cgcuauuugg aggcauguca ugguuuaguc aaauucuuau agguacuuua     1980

cuaauguggu uaggguuaaa uacgaaaaac gggucaauaa guuuaguaug uuuagcucua     2040

ggaggagugc ucauauuccu aucaacagcg guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9302
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 3 opt

<400>  9302
gggagaaagc uuaccaugac aaguguagga auuguaggau uauuacugac aacugcuaug       60

gcagcagagg uuacaagaag aggaagcgca uauuauaugu aucuagauag aucagaugcu      120

ggugaagcaa uuucuuuucc aacaacauua ggaaugaaua aauguuacau acaaauuaug      180

gauuuaggac auauguguga cgcuaccaug aguuaugaau guccaauguu agaugaagga      240

guugaaccag augacguaga uuguuggugu aauacaacau caacaugggu aguauauggg      300

acaugccauc auaagaaagg agaagcaaga cgaaguagac gagcuguaac auugccauca      360

cauucaacaa gaaaauugca aacuagauca caaacuuggc uagaaaguag agaauauaca      420

aaacauuuaa uccgaguaga gaauuggaua uuccgaaauc cuggauuugc auuagcagca      480

gcagcaauag cguggcuucu cgguaguuca acaucacaaa aaguuauaua cuuggucaug      540

auauuauuaa uagcuccggc auauagcauc agauguaucg gaguaaauaa ucgagauuuc      600

guagaaggaa ugucaggugg aacgugggua gauguaguau uagaacacgg uggaugugua      660

acuguaaugg cacaagauaa accaacuguu gauauagaac ucguaacaac gacaguaagu      720

aauauggcgg aaguacgaag uuauuguuau gaagcaucca uaucugauau ggcaucagac      780

agcagauguc caacccaagg agaagcauac cuggauaaac aaucagauac gcaauaugua      840

uguaaaagaa cuuuaguuga cagaggaugg ggaaauggau gcggcuuauu cggcaaagga      900

aguuuaguaa caugcgcaaa auuugcgugu aguaagaaaa ugacaggaaa aagcauacag      960

ccagaaaacc uagaauauag gauaauguug aguguacaug gcagucaaca uaguggaaug     1020

auagucaaug auacuggcca cgaaacagau gaaaauaggg caaaaguaga aaucacacca     1080

aauagcccuc gagcagaagc aacauuagga ggauuuggau cuuuaggucu agauugugaa     1140

ccaagaacag gcuuagauuu uucagacuua uacuaucuua caaugaauaa caaacauugg     1200

cuaguacaua aagaaugguu ucacgauaua ccacuaccuu ggcaugcagg agcagacacu     1260

ggaacaccac auuggaauaa caaagaagca cucguagaau uuaaagacgc acaugcaaaa     1320

agacagacag ugguaguuuu aggaucacaa gagggagcag uacauaccgc auuagcagga     1380

gcucucgaag cugaaaugga uggagcaaaa ggaagauuau caagcggaca uuuaaaaugc     1440

agacuaaaaa uggacaaauu aagauuaaaa gguguaaguu acagucuaug uacugcagca     1500

uuuacauuca cuaaaauacc agcggaaacc uuacauggaa cgguaacagu agaagugcag     1560

uacgcuggua cggauggacc cuguaaaguc ccugcacaaa uggccguaga uaugcaaacu     1620

cuaacaccug uaggacgacu aauuacagcu aauccaguaa uaaccgaaag cacagaaaac     1680

ucuaaaauga uguuagaauu agauccuccu uuuggagauu cauacauugu uauaggagua     1740

ggcgaaaaga aaaucacaca ccauuggcau agauccggaa guacaauagg aaaagcauuu     1800

gaggccacag uaagaggcgc aaaaagaaug gcaguacuag gagacacagc cugggauuuu     1860

gguucuguag gaggagcauu aaacaguuua ggcaaaggaa uacaucagau uuucggagca     1920

gcauuuaaau cauuauucgg gggaaugagu ugguuuucac aaauacuuau aggaacacua     1980

cuaauguggc uggguuuaaa uacuaaaaau ggaagcauau cccuaguuug ccuagcacua     2040

ggaggaguuc ugauauuucu aucaacagcu guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9303
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 3 opt

<400>  9303
gggagaaagc uuaccaugac gucaguagga auaguagggu uacuuuuaac gacagcaaug       60

gcaguagaag uaacacgaag aggauccgca uauuacaugu aucuugaccg aucagaugca      120

ggagaagcaa uaaguuuccc aacaacacua ggaaugaaua aaugcuauau acaaauaaug      180

gauuuagguc acaugugcga ugcaacaaug uccuaugaau guccgauguu agaugaaggu      240

guggaaccug augauguaga uuguuggugu aauacuacau cuacaugggu ugucuacggc      300

acaugucauc auaagaaagg agaagcgcga aggaguagga gagcaguaac acuaccguca      360

cacagcacaa gaaaauuaca aacuagauca caaaccuggu uggaauccag agaauauaca      420

aaacauuuaa ucagaguaga aaauuggaua uuuagaaauc caggguuugc uuuagcagca      480

gcagcaauag caugguuauu aggaaguuca acuagccaaa aaguuaucua ccuaguaaug      540

auucuauuaa uagcaccagc guauucaaua agauguauag ggguaaguaa uagagacuuc      600

guagaaggaa ugaguggagg aacuugggua gacgugguau uagaacaugg aggaugcgua      660

acagugaugg cacaagacaa accagcagua gacauagaau uaguaacaac aacaguaagc      720

aauauggcag aaguaagauc cuauuguuau gaagcaucaa uaucagauau ggcaagcgac      780

ucgagauguc cgacacaagg agaggcuuau cuagacaaac aaagcgauac ccaauaugua      840

ugcaaaagaa cauuaguaga uagaggaugg gguaaugguu gcggauuauu cggaaaagga      900

ucucuaguca caugugcuaa auucgcaugu ucaaagaaaa ugacaggcaa aaguauacaa      960

ccagaaaauu uagaauacag aauaauguua agcguucaug gaagucaaca uaguggaaug     1020

auaguaaacg auacgggaca cgaaacggau gaaaauagag cgaaagugga aauaacaccu     1080

aauucaccaa gagcagaagc uacacuaggu ggauuuggau cucuaggacu ggacugugaa     1140

cccaggacag gucuagauuu uagcgaccua uauuacuuaa cgaugaauaa uaaacauugg     1200

cuaguucaua aagaaugguu ucaugacaua ccauuaccau ggcaugcugg agccgacaca     1260

gguacaccac auuggaauaa uaaagaagca uuaguagagu ucaaagaugc ucaugccaaa     1320

aggcagacag uuguuguacu agggucacaa gaaggagcag uacauacagc ucuagcggga     1380

gcacuagaag cagaaaugga uggagcaaaa gggcgauuaa gcucuggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu aagauuaaaa ggaguaaguu auucauuaug cacagcggcc     1500

uuuacauuca caaaaauacc agcagaaaca cuacauggga cagugacagu agaaguucaa     1560

uaugcaggaa cagacggacc uugcaaaguu ccggcacaaa uggcaguuga uaugcaaaca     1620

cuaacaccag ugggaagauu aauaacagca aacccaguua uaacagaaag uacagaaaau     1680

agcaaaauga ugcuggaauu agauccacca uuuggugaca guuacauagu uauaggagug     1740

ggagaaaaga aaauaacaca ucauuggcau agaucuggga gcacaauugg aaaagcguuc     1800

gaagccacag uaaggggugc aaaaagaaug gcaguauuag gagauacugc augggauuuu     1860

ggaucaguag ggggugcuuu aaauucauua ggaaaaggaa uacaucaaau uuucggugca     1920

gcauuuaaaa guuuauuugg aggaauguca ugguucaguc aaauccucau uggaaccuua     1980

uuagucuggc ugggauuaaa uacuaaaaac gguucuauaa guuuaaccug ucuagcacua     2040

ggaggcguau uaauuuucuu aagcacagca guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9304
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 3 opt

<400>  9304
gggagaaagc uuaccaugac gucaguaggg auagucggau uauuauuaac aacugcuaug       60

gcaguugaag uuacaagaag aggaagugca uacuauaugu aucuugauag aucagaugcg      120

ggugaagcaa uaucauuucc uacuacacuu ggaaugaaca aauguuauau acaaauaaug      180

gaucuaggac auauguguga ugcaacaaug agcuacgaau guccgauguu agacgaagga      240

guagagccag acgauguaga uuguuggugu aacacaacau caacaugggu cguuuaugga      300

acgugucacc acaaaaaagg agaagcaaga agaucgcgaa gagcaguuac acuaccaagc      360

cauaguacca gaaaacuaca aaccagauca caaacauggc uagaaucacg ugaauauaca      420

aaacaucuaa uacgaguuga aaauuggaua uuuagaaauc cagguuuugc acuagcagca      480

gcaguaauag cauggcuacu aggaucguca acaagucaaa aagucauaua cuuagucaug      540

auccuacuaa uagcaccagc uuacuccaua agauguauag gaguaagcaa cagggauuuu      600

guugaaggua ugaguggcgg aacauggguu gauguuguac uagaacacgg ggguugugua      660

accguuaugg cacaagacaa accaacagua gacauagaac ugguaacaac aacaguauca      720

aauauggcag aaguucgaag uuauuguuau gaagcaagua uuagcgacau ggcuagugau      780

ucaagauguc caacacaagg ugaagcauau uuagauaaac aaucugauac acaauacguc      840

uguaaaagaa cacuugucga cagaggaugg ggaaauggau gugguuuauu uggaaaagga      900

agccucguaa cuugugcuaa auuugcaugu aguaaaaaaa ugacgggaaa aaguauacaa      960

ccagaaaauu uagaauauag aauaauguua agugugcacg gaucacagca uucagguaug     1020

auuguaaacg acacaggaca ugagacugac gaaaauagag caaaaguaga aauaacaccg     1080

aacagcccua gagcagaagc aacacuugga gguuuuggau cauuaggucu ugauugugaa     1140

ccuagaacag guuuagauuu cagcgacuua uauuaucuaa caaugaacaa uaaacauugg     1200

uuagugcaca aagaaugguu ucacgacaua ccgcugccau ggcaugcagg agcagacaca     1260

ggaacaccuc auuggaauaa uaaagaagcg cuaguugaau uuaaagaugc acacgcaaaa     1320

agacaaacag uaguuguacu aggaagccaa gaaggcgcag uucauacagc guuagcagga     1380

gcucuagagg cagagaugga uggcgcaaaa gggagauuaa guaguggaca uuugaaaugu     1440

agacuaaaaa uggauaaauu aagauuaaaa ggaguaucau auagccuaug cacagcagcc     1500

uuuacauuua caaaaauacc agcagaaaca uuacauggaa cuguaacugu ggaaguacaa     1560

uaugcaggaa cagauggccc auguaaagua ccagcacaaa uggcugucga caugcaaaca     1620

cuaacaccag uuggaagguu gauaacugca aauccaguga uuacagaauc aacagaaaau     1680

ucaaaaauga uguuagaguu agacccacca uuuggagaua gcuacauagu aauuggcgua     1740

ggagaaaaaa aaauuacgca ucauuggcac agaagcggau ccacaauagg aaaagcauuu     1800

gaagcaacag uaagaggagc aaaaaggaug gcaguguuag gagauacagc augggauuuu     1860

ggaagugucg gaggugcucu aaacucauua gguaaaggua uacaucaaau cuuuggggca     1920

gcauuuaagu cgcuauuugg aggaaugagc ugguuuagcc aaaucuuaau ugguacacua     1980

cuagucuggc uaggauuaaa cacaaaaaau gguucaauaa gcuuaaccug ucuagcauua     2040

gggggcguac uaaucuucuu aucaacagca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9305
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 3 opt

<400>  9305
gggagaaagc uuaccaugac aucagugggu aucguaggau uauuacuuac aacagcaaug       60

gcaguugaag uaacaaggag gggauccgca uauuauaugu acuuagacag aagugacgcc      120

ggugaagcaa uaucauuucc aacaacacua ggaguuaaua aauguuauau acaaauuaug      180

gaccuaggac acauguguga ugcaacaaug uccuacgaau guccaaugcu agacgaagga      240

guggaaccag augauguaga uuguuggugu aauacaacaa guacaugggu aguuuauggg      300

acaugucauc auaagaaagg agaagcacga agaucaagga gagcugucac auuaccauca      360

cacaguacga gaaaacuaca aacaagaagu caaacauggc uagaaucaag ggaauauacu      420

aaacaucuua uaaggguuga aaauuggaua uucagaaauc caggauucgc acuagcagcu      480

gcagcuauag cguggcuuuu ggguaguagu acuagccaaa aagucauaua uuugguuaug      540

auucuacuaa uugcaccggc auauuccaua cguuguauag gaguaucaaa uagagacuuu      600

guugaaggaa ugucaggagg aaccugggua gauguaguac uagaacacgg aggcuguguu      660

acggucaugg cacaagauaa accaacagua gauauagaac ugguuacaac aacaguaagc      720

aauauggcug aaguacgaag cuacugcuac gaagcaagua uaucagauau ggcaucagac      780

ucacgauguc caacacaagg agaagcauau uuagauaaac aaagugauac ucaauaugua      840

uguaaaagaa cucuaguaga uagaggaugg gggaaugggu gcgguuuauu cggaaaaggu      900

agucuaguua ccugugccaa auuugcaugc ucaaagaaaa ugacuggaaa aaguauacag      960

ccugaaaauu uagaauauag aauaaugcua ucaguacaug gaagucaaca uuccggaaug     1020

auagucaaug auacaggaca ugaaacagau gaaaauagag caaaaguaga aauuacacca     1080

aacaguccaa gagcagaagc aacauuagga gguuuugguu cauuaggauu agauugcgaa     1140

cccagaacag gacuagacuu uagugacuua uauuaccuaa caaugaauaa uaaacauugg     1200

uuagugcaua aagaaugguu ucaugauaua ccauuaccau ggcacgcagg agcugauacg     1260

ggaacaccac acuggaauaa uaaagaggcu cugguagaau uuaaagaugc acacgcaaaa     1320

agacaaacag uaguuguuuu ggguagccaa gaaggugcag uucacacagc guuggccgga     1380

gcauuagagg ccgaaaugga uggugcaaaa ggaagacuau caaguggaca ucuaaaaugu     1440

agacuaaaaa uggacaaauu aagauuaaaa ggaguaaguu auagccuaug cacagcugca     1500

uuuacauuca caaaaauacc agccgaaaca uuacauggaa caguaacagu agaaguucaa     1560

uaugcaggaa cagacggacc augcaaagua ccugcacaaa uggcugucga uaugcaaaca     1620

uuaacuccag uuggaagauu gauaacagca aauccaguaa uaacagaaag uaccgaaaac     1680

agcaaaauga uguuagaacu ugauccaccu uuuggugaua guuauauagu gauuggcgua     1740

ggagauaaga aaauuacaca ccauugguau agaucaggau caacaauagg aaaagcauuc     1800

gaagcaacug uaagaggagc aaaacgaaug gcaguauuag gagacacugc augggauuuu     1860

ggaagcguag gaggagcguu aaauucacua ggcaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaaa guuuguuugg cggaaugagc ugguuuaguc aaauauuaau aggaacauua     1980

uuaguauggu uaggacuaaa cacgaaaaau ggaucaauaa gucuaacaug uuuagcucua     2040

gguggaguau uaauauuuuu aaguacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9306
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 3 opt

<400>  9306
gggagaaagc uuaccaugac aucaguugga auaguuggac uauuacucac uacagcaaug       60

gcaguagagg uaacacgaag aggcucagca uauuauaugu accuugauag gucagaugcc      120

ggaaaagcaa uuucauuucc aacaacauua gguguaaaca aaugcuauau acaaauaaug      180

gaucuaggcc auauguguga ugcaacuaug ucauaugaau gcccaauguu agaugaagga      240

guagaaccag augauguaga cuguuggugu aauacuacaa guacuugggu aguauaugga      300

acaugucacc auaaaaaagg agaagcaaga agaagcagga gagcagucac auugcccuca      360

cauucuacaa gaaaauugca aacgagaagc caaacuuggu uggaaucaag agaauacaca      420

aaacacuuaa uaagaguaga aaauuggaua uuuagaaacc caggauucgc auuggccgca      480

gcagcuauag caugguuauu aggaucuucu acgucgcaaa agguaauaua ucuaguaaug      540

auauuauuaa uagcccccgc guauucaauu agauguauug gaguaucaaa uagagacuuc      600

guagaaggga ugucaggagg aacauggguc gauguuguuu uagaacacgg uggaugcguc      660

accguuaugg cacaagauaa accuacaguc gauauugaac uaguaacuac uacugucuca      720

aacauggcag aaguucgaag uuauuguuau gaagcaucaa uuucagauau ggcaucagau      780

aguagauguc caacccaagg agaagcuuau uuagacaaac aaagcgacac acaauauguu      840

uguaaaagaa cauuaguuga uaggggcugg ggaaaugguu guggauuauu cggaaaagga      900

aguuuaguua caugugcaaa auucgcuugu ucuaaaaaaa ugacgggaaa aaguauccaa      960

ccugagaauu uagaauauag aauaaugcua ucaguacacg gaagucaaca cagcgggaug     1020

auaguaaaug acauaggaca ugaaacggau gaaaauaggg cuaaaguaga aauaacaccg     1080

aacucaccaa gagccgaagc aacacuuggu gguuucggau cauuaggacu ggauugcgaa     1140

ccaagaacug gauuagacuu cagcgaucua uauuacuuaa caaugaauaa uaaacacugg     1200

uuaguacaua aagaaugguu ccaugauaua ccauuacccu ggcaugcagg agcagauacg     1260

ggaacaccac auuggaauaa caaagaagcc cuaguagaau ucaaagaugc acaugccaaa     1320

agacaaacag uaguaguucu agggagucaa gaaggagcag uacauacugc acuagcagga     1380

gcauuggaag cagagaugga uggagcaaaa ggaagauuau caagugguca uuuaaaaugc     1440

agauugaaaa uggauaaauu acgauuaaaa ggaguaucau acucucuuug cacagcagca     1500

uuuacuuuca cuaaaauucc agcugaaacu cuucauggaa caguaacagu agaaguccag     1560

uacgcgggaa cagauggacc augcaaagua ccugcacaaa uggcaguaga caugcaaaca     1620

uuaaccccag uaggaagauu aauaacagca aacccuguaa uaacagaaag uacugaaaac     1680

ucuaaaauga uguuagaauu agauccaccu uuuggagauu cauauauagu cauaggaguu     1740

ggagauaaaa aaauaacaca ccacuggcau aggucaggaa gcacaauugg aaaagcauuu     1800

gaagcuaccg uaagaggcgc aaaaagaaug gcaguauuag gagacacagc augggauuuc     1860

gguucuguag gaggugcguu aaacucauug gguaaaggga uacaccaaau cuuuggagcc     1920

gccuuuaaga gccuauuugg aggaaugagu ugguuuaguc aaauacuaau aggaacacua     1980

uuaguauggu ugggacuaaa uacaaaaaau ggaagcauau cacuuacaug uuuagcauua     2040

ggagguguau uaauauuucu gucaacugca guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9307
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 3 opt

<400>  9307
gggagaaagc uuaccaugac aaguauagga auuguagguc uacuucucac uacagcaaug       60

gcagcugaga uaacaagacg ggguagugca uacuauaugu accuagacag aagugacgca      120

gguaaagcaa uuucauuugc aacuacauua ggcguaaaca aaugucaugu acaaauaaug      180

gauuuaggac auaugugcga cgcaacaaug ucauaugaau gcccuaugcu ggaugaggga      240

guugaaccug acgauguaga uuguuggugu aauacaacau caacaugggu agucuacgga      300

acaugccauc auaagaaagg agaagcaaga agaaguagga gagcagugac acuaccuucu      360

cauaguacaa gaaaauuaca aacuagaagu caaacauggc uagaaucaag agaauauaca      420

aaacauuuaa uaaaaguuga aaauuggaua uuuagaaauc caggauuuac auuaguagcu      480

guugcaauag cauggcuauu gggaucaagu accagucaaa aaguuauuua ucuaguaaug      540

aucuuauuaa uagccccugc auauagcaua cgauguauag gaguuucaaa cagagauuuu      600

guagaaggaa ugaguggagg uaccugggua gauguaguau uagaacaugg aggcugcgua      660

acggugaugg cucaagauaa gccaacaguu gauauagaau uaguaacaac aacaguaagc      720

aacauggcag aaguuagaag cuacuguuac gaagcaagua uaagugauau ggcaucagau      780

aguagaugcc caacgcaagg agaagccuac uuagauaaac aaagugauac acaauaugua      840

ugcaaaagaa cauuaguuga ucgaggaugg ggaaacgguu gcgguuuauu uggaaaagga      900

ucuuuaguaa cuugugcaaa guuuacaugu aguaagaaaa ugacugguaa aagcauacaa      960

ccagaaaauc uagaauauag aauaauguua ucaguacaug gaagccaaca cagcggaaug     1020

auaguuaaug acaccggaca cgaaacagac gaaaauagag ccaaaguaga aguuacacca     1080

aacaguccuc gagcggaagc aacacuaggc ggauucggaa guuuaggauu agacugugaa     1140

ccuagaacag gccuagauuu uagugacuua uacuacuuaa caaugaauaa caaacauugg     1200

uuaguacaca aagaaugguu ccaugacauc ccacuaccau ggcacgcugg ugcagauacu     1260

ggaacaccac auuggaauaa uaaagaagca uuaguagaau uuaaagaugc acaugcuaaa     1320

agacaaacag uuguuguuuu aggaucucaa gaaggagcag uacauacagc acuagcuggc     1380

gcucuggagg cugaaaugga uggagcgaaa ggaagauuau ucucaggaca cuuaaaaugu     1440

agauuaaaaa uggauaaauu gagacuaaag ggaguaaguu auagucuaug uacagcagca     1500

uuuacguuua caaaagugcc ugcagaaaca cuacauggaa cugucacggu agaaguucaa     1560

uaugcaggaa cugauggacc guguaaagua ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaaccccug uaggaagacu gauaacagca aacccgguaa uaacagaaag uacagaaaac     1680

aguaaaauga uguuagaauu ggacccgcca uucggagaua gcuauauagu aauuggcgua     1740

ggagauaaga aaauaacuca ccauuggcac agaaguggau caacaauagg aaaagcauuc     1800

gaagcaacag uaagaggagc aaaacgaaug gcaguauugg gagauacagc augggauuuu     1860

ggaucaguag gaggcguauu uaacucgcua ggaaaaggga uacaucaaau cuuuggagca     1920

gccuucaaaa guuuauuugg aggaaugagc ugguuuaguc aaauacuuau aggaacacua     1980

uuaguauggu uaggauuaaa cacuaaaaac ggaaguaucu cuuuaacaug uuuagcauua     2040

ggaggaguga ugauauucuu aucgacagcu gugagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9308
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 3 opt

<400>  9308
gggagaaagc uuaccaugac aaguauagga auaauagguc ucuuguuaac gacagcuaug       60

gcagcagaaa uaacaagacg agggagugca uauuauaugu accuagaccg uucugacgcc      120

ggaaaagcua uaucauuugc cacaaaccuc ggaguaaaca agugucaugu acaaauaaug      180

gaucucgguc auauguguga cgcgacuaug aguuaugaau guccaaugcu agaugaagga      240

guagaaccag acgacguuga uugcuggugu aauacaacaa gcacuugggu aguauaugga      300

acaugucauc acaagaaagg ggaggccaga agaucaagac gagcaguaac auuaccaucc      360

cacagcacaa gaaaauuaca aacaagaagu caaacauggu uagaaaguag agaauauacu      420

aaacauuuaa uaaaaguuga aaauuggauu uucagaaauc caggauucgc auuagcagca      480

guagcaauag ccuggcuauu aggaucauca acuucacaaa aaguaauuua ucuggucaug      540

auauuacuaa uagcaccagc auauagcaua agaugcauag gaguaaguaa cagagauuuu      600

guagaaggaa ugaguggagg aacauggguu gaugucguau uagaacaugg agguugugua      660

acuguuaugg cgcaagauaa accaacagua gacaucgaau uaguuacaac aacaguaagc      720

aauauggcug aaguaaggag uuauuguuau gaagcaucca uuucggauau ggccucagau      780

aguagaugcc cuacacaagg agaagcauau uuagauaaac aaucagacac acaauauguc      840

ugcaaacgaa cucuagucga uagaggaugg gguaauggcu guggccuauu cgguaaagga      900

ucccuaguaa caugcgcaaa auucacaugc ucuaagaaaa ugacagggaa aucaauccaa      960

ccugaaaauu uggaauaucg aauaauguua aguguacacg ggagucaaca cucaggaaug     1020

auaguaaaug auauagggca ugaaacagau gaaaacagag caaaaguaga aguaacgccu     1080

aauucaccaa gagcugaagc aacacuaggu ggauuuggga guuuaggauu agacugugaa     1140

ccaagaacag gauuagauuu cucagaccuc uacuacuuaa caaugaauaa uaaacacugg     1200

cuaguacaua aagaaugguu ccaugauauu ccuuuaccau ggcacgcagg agcagauaca     1260

ggcacaccuc auuggaacaa uaaagaagcu cuaguagaau uuaaagaugc acaugcaaaa     1320

agacaaacug ugguuguauu agguucacaa gaaggagcag uacauacagc acuagcuggc     1380

gcauuggaag cagaaaugga cggagcaaaa gguaggcucu uuaguggaca ucuaaaaugu     1440

agguuaaaaa uggacaaacu uagguuaaaa ggaguaucau acucacuaug cacugcugcu     1500

uucacauuca caaaaguacc cgcagaaaca uuacacggaa cagugacagu ugaaguacaa     1560

uaugcuggaa cugauggucc cuguaaggua ccagcacaaa uggccguaga uaugcaaaca     1620

uuaacuccag uagguagacu aauaaccgca aauccaguaa uuacagaauc cacagaaaau     1680

aguaagauga ugcuagagcu agauccacca uuuggagaua guuauauagu cauaggagua     1740

ggugacaaga aaaucacaca ucacuggcau agaaguggca gcacaauugg aaaagcauuu     1800

gaagcaacag uaagaggagc aaaaagaaug gcaguauuag gugauacagc augggauuuu     1860

ggaaguguag guggaguuuu uaauucacua ggaaaaggag uacaucaaau auuuggagcc     1920

gcuuucaaau cacuauuugg aggaaugagu ugguuuucuc aaauauuaau aggaacauua     1980

uuaguauggu uaggucuaaa cacuaaaaau ggaucaaucu cauuaacaug uuuagcacuc     2040

ggaggaguua ugauuuuccu aagcacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9309
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 3 opt

<400>  9309
gggagaaagc uuaccaugac uucaauaggu auaauagguc uauuacuaac aacagcaaug       60

gcugcagaaa uaacacgaag aggaucagcu uacuacaugu aucuagacag aagugaugcu      120

ggaaaagcaa uuagcuucgc aacaacacuc ggaguuaaua aaugccacgu ucaaauaaug      180

gacuuaggac auauguguga ugcaacuaug aguuacgaau guccaaugcu ugacgaaggu      240

guugaaccag augauguaga cuguuggugu aauacgacua gcacgugggu aguauacgga      300

acaugucauc auaaaaaagg cgaagcuaga agaagucgac gagcaguuac acuaccgagu      360

cacaguacaa gaaaacuaca aacacgauca caaacauggu uagaaaguag agaauauaca      420

aaacaccuaa uaaagguuga gaauuggauc uucagaaauc cgggguucgc ucuuguagca      480

guagccauag caugguuauu aggaucuagc acaucacaaa aaguaaucua ucuaguuaug      540

auacuuuuga uagcaccagc uuauaguaua agauguauug gaguaucaaa uagagacuuc      600

guagaaggaa ugucuggagg aaccugggua gauguaguau uagaacacgg cggaugcguu      660

acaguaaugg cccaagauaa accaacagua gacauagaau uaguaaccac gacaguaagu      720

aauauggcgg aagugagaag uuauugcuau gaagcaagua uaucagauau ggcaucagau      780

ucacgaugcc caacacaagg agaagccuau uuagauaaac aaagcgauac acaauacguu      840

ugcaaaagaa cucuaguaga cagaggaugg ggaaauggau guggauuauu cggaaaagga      900

ucuuuaguaa ccugugcaaa auuuacaugc aguaaaaaaa ugacagguaa aaguauacaa      960

ccagaaaauu uagaauauag aaucaugcuu ucaguacacg gaucacaaca uagcgguaug     1020

auaguaaacg acauaggaca ugaaaccgau gaaaauaggg caaaaguaga gguaacacca     1080

aacucaccaa gagcagaagc uacuuuagga ggauuuggau cauuaggguu ggauugugaa     1140

ccacgaaccg gacuagauuu uagugaucua uacuaucuaa caaugaauaa caaacauugg     1200

uugguacaua aagaaugguu ucacgacauu ccucuaccau ggcaugcugg ugcagauacu     1260

ggaacaccac auuggaauaa uaaagaagca cuaguagagu uuaaagaugc ccaugccaaa     1320

cgacaaacag uaguaguacu aggaagucaa gaaggagcag uacauacagc auuagcugga     1380

gcauuagaag cggaaaugga uggagcaaaa ggaagacuau ucagugguca ccuaaaaugu     1440

agauuaaaaa uggauaaauu aagauuaaaa ggagugucau auucccuaug cacagcggca     1500

uucacauuua cgaaaguucc agcagaaaca cuccauggaa cagugacagu agagguacaa     1560

uacgcuggaa cugauggucc augcaaagua ccggcacaaa uggcgguuga uaugcagaca     1620

uuaacaccag uagggagguu aauaacagca aauccaguaa uuacagaaag cacagaaaac     1680

ucuaaaauga ugcuagaguu agauccaccc uuuggagaca guuacaucgu aauaggagua     1740

ggagauaaaa aaauaacgca ucauuggcau agaucagguu cuacuauugg caaagcuuuu     1800

gaggcaacag uaagaggagc aaaaaggaug gcagucuuag gugauacggc augggauuuu     1860

ggcucuguag gaggaguuuu uaacaguuua gguaaaggaa uucaccaaau auuuggagcg     1920

gcauuuaaaa gcuuauuugg aggaaugagu ugguuuucac aaauacuuau agguacguua     1980

uuagucuggc uuggauuaaa cacaaaaaau ggaaguauau cacuaacuug cuuagcauua     2040

ggaggcguaa ugauauuuuu aaguacugca guaagugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9310
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 3 opt

<400>  9310
gggagaaagc uuaccaugac aagcauugga auuauaggac uauuauuaac aaccgcaaug       60

gccgcagaaa uaacgagacg uggaagcgca uacuauaugu auuuagaccg aucugaugca      120

ggaaaagcua uuucuuuugc gacaaauuua ggaguuaaua aaugccaugu ucaaauaaug      180

gaucuaggac auauguguga ugcaacaaug ucauaugaau guccaauguu agaugaagga      240

guagaaccag acgacguaga uuguuggugc aacacaacau caacaugggu aguauacgga      300

acgugucacc auaagaaagg ugaagcaaga agaucuagga gagcaguaac auuaccaucg      360

cauagcacaa gaaaacuaca aacaagaagu caaaccuggc uagaaucaag agaauacaca      420

aaacaccuca uaaaagugga aaauuggaua uucagaaacc caggauucgc uuuagcagca      480

guagcaauag cauggcuuuu aggaagcagu acaucccaaa aaguaauaua uuuaguuaug      540

auauuauuaa uagcuccagc auacucaauc aggugcauag gaguaaguaa uagggauuuc      600

guugaaggaa ugucaggagg aacaugggua gacguaguau uagagcacgg agguugcgua      660

acgguaaugg cacaagacaa accaacagug gauauagaau uaguaacaac aacuguuagu      720

aauauggcgg aaguacgauc auauugcuau gaggccucua uaucagacau ggcaucagac      780

agcagaugcc caacccaagg agaggcuuac uuagauaaac aaagugacac acaauacgua      840

uguaaaagaa cacucguuga ucgaggcugg ggaaauggau gcggauuauu uggaaaagga      900

ucuuuaguua caugcgcaaa auuuacaugu aguaagaaaa ugacagggaa aagcauacaa      960

ccagaaaauu uagaauauag aauaaugcua ucuguacaug gaucacaaca uucagggaug     1020

auaguaaacg augaaaaccg agcaaaaguc gaaguaacuc caaauucacc ucgagcggaa     1080

gcaacguuag gugguuucgg uaguuuaggu uuagauugcg aaccuagaac aggacuggau     1140

uucagcgauu uauauuaccu aacuaugaac aauaaacauu gguuagugca uaaagaaugg     1200

uuucacgaua uaccacuccc auggcacgca ggagcugaua caggaacacc acauuggaau     1260

aauaaagaag cucuaguaga auuuaaagau gcacacgcaa aaagacaaac aguaguggua     1320

cuaggaaguc aagaaggagc cguacauaca gccuuagcag gagcauuaga agcugaaaug     1380

gauggugcaa aaggaagguu auuuucagga cauuuaaaau guagacucaa aauggauaaa     1440

uuaagauuaa aagguguaag uuauucacua ugcacagcag cuuuuacauu uacaaaagua     1500

ccagcagaaa cacuacacgg gacagugaca guagaaguac aguacgcagg aacagauggg     1560

ccauguaaag uaccagcaca aauggcugua gacaugcaga cauugacacc gguuggaaga     1620

uuaauaacag caaauccagu aauaacugaa aguacugaaa auucaaaaau gaugcuagaa     1680

cuagacccac cauuuggcga cuccuauaua guuauaggag ugggugauaa gaaaaucaca     1740

caucauuggc auagaucagg aaguacgaua ggaaaagcgu uugaagccac gguuagagga     1800

gcuaaaagaa uggcagugcu aggagauacg gcuugggauu uuggaagugu aggaggcgug     1860

uuuaauagcc uagggaaagg aauacaucaa auauuugggg cagcuuucaa aagucuauuu     1920

ggagguauga guugguuuag ucaaauauua auagguacac uauuaguaug guuaggauug     1980

aacacuaaaa augggagcau uaguuugacu uguuuagcac uagguggugu aaugauauuu     2040

uuaucaacag caguaagugc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  9311
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 3 opt

<400>  9311
gggagaaagc uuaccaugac cucaauuggu auugucggac uacuccuaac aacagcaaug       60

gcagcagaaa ucacucgaag gggaucggcg uacuauaugu aucuugauag gagcgacgca      120

ggaaaagcaa ucucguuugc uacaacauua ggaguaaaca aaugccaugu acaaauaaug      180

gauuugggac auaugugcga ugcaacaaug uccuaugagu guccuauguu ggacgaagga      240

guugaacccg acgacguaga cuguuggugu aauacgacaa gcacaugggu uguauauggu      300

acaugucauc auaaaaaagg ugaagcuaga agaucucggc gagcagugac auuaccauca      360

cauucaacaa ggaaacuaca aacuagauca caaacauggu uagaaucacg agaauauacg      420

aaacaucuaa ucaaaguaga aaacuggauu uuucguaacc caggauuugc ccuuguagca      480

guagcaauag cauggcuucu aggaucgagu acaucccaaa aaguaauuua uuuaguaaug      540

auacuauuaa uagcaccagc auauagcaua agauguauag gaguuaguaa cagagacuuc      600

gucgaaggaa ugucaggcgg aacauggguu gauguagucc uagaacacgg cggaugugua      660

acaguaaugg cucaagauaa accaacagua gauauagaac ucgucacgac aacaguauca      720

aauauggccg aaguaagaag uuauuguuau gaagcgagua uuagugacau ggccucagau      780

ucaagauguc caacccaggg cgaagcauau cuugauaaac aaucugacac acaauaugua      840

ugcaaaagaa cacuuguaga cagaggaugg ggaaauggau gcggacuauu uggaaaagga      900

agccuaguaa caugugcaaa auucacuugu ucaaaaaaga ugacaggaaa aagcauucaa      960

ccugaaaacu uagaauaucg aauaauguua ucuguucaug gaucacaaca uucuggaaug     1020

auaguaaaug acacuggaca ugaaacugac gaaaacagag caaaaguaga agucacaccc     1080

aauucaccaa gagcagaagc uacacuagga ggauuuggua gucuaggucu agauugugaa     1140

ccaagaacug gacuagauuu cucagacuua uacuaucuga caaugaauaa caaacacugg     1200

uuaguacaua aggaaugguu ucaugacaua ccucucccuu ggcacgcagg agcagauaca     1260

gguaccccac auuggaacaa uaaagaagca cuaguagaau uuaaagacgc ccaugcaaaa     1320

agacaaacag uaguagucuu aggaagccaa gaaggagcag uacauacagc auuagcagga     1380

gcucuagaag ccgaaaugga cggagcaaaa gguagacuau uuucugguca ucuaaaaugc     1440

cgacuaaaaa uggacaaauu aagacuaaaa gguguaucau acagcuuaug cacagccgca     1500

uuuacauuca cuaaaguccc agcagaaaca uuacacggga caguaacagu agaaguacaa     1560

uaugcaggaa cagacggacc augcaaaguc ccagcacaaa uggcaguaga caugcaaaca     1620

uuaacuccag uaggaagacu aauaacagcu aacccaguua uuacagaauc gacagaaaau     1680

ucaaaaauga ugcuagaacu ugauccacca uuuggugauu cauauauagu uauaggagua     1740

ggagauaaaa aaauaacaca ucauuggcau agaucaggau caacaauagg aaaagcauuu     1800

gaagcaacag uaaggggcgc caaaagaaug gcaguauuag gagacacagc augggauuuu     1860

gguucuguug gagguguauu caauucauua ggaaaaggaa uccaccaaau auuuggagcc     1920

gcauuuaaau cucuauuugg aggaauguca ugguucucac aaauauuaau aggaacauug     1980

uuaguauggc uaggauuaaa cacgaaaaau gggucaauua gucuaaccug ucuagcauug     2040

ggaggaguaa ugauauuucu aucaacagca guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9312
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 3 opt

<400>  9312
gggagaaagc uuaccaugac aucuaucggu auaguaggau uacuauuaac aacggcaaug       60

gccgcagaga uaacucgaag aggaucagcg uacuauaugu acuuagauag gucugacgcc      120

ggaaaagcaa uaaguuuugc uacuacauua ggaguaaaua aaugucacgu acaaauaaug      180

gaucuaggac auauguguga cgcuacuaug ucauaugaau gcccaauguu agaugaaggc      240

guugaaccgg augauguaga uuguuggugc aauacuacau caacaugggu aguauaugga      300

acaugucaac auaagaaagg ugaagcccga agaagucgga gagcugucac auuaccauca      360

cacucaacaa gaaaauuaca aacaagauca caaacauggu uagaauccag agaauacaca      420

aaacaucuaa uaaaaguuga gaacuggauu uuuagaaacc caggcuuugc uuuaguagca      480

guggcaauag cgugguuauu gggaaguagu acaagucaaa aagugauaua uuuaguaaug      540

auucuauuaa uagcuccagc auauaguauc agauguauag guguaucaaa uagagauuuu      600

guagaaggaa ugagcggagg aaccugggua gaugucguau uagaacaugg aggaugugua      660

acuguuaugg cacaagacaa accuaccgua gauauagaau uaguaacaac uacuguuagu      720

aauauggcug aagucagaag cuauugcuac gaagcaagua uaagugauau ggcaagugau      780

ucgagauguc caacucaagg cgaagcauau cuugauaaac aaagcgauac acaauaugua      840

uguaaaagga cguugguaga uagagguugg ggaaacggau guggcuuauu cggaaaagga      900

agucuaguua cuugcgcgaa auuuacaugu agcaagaaaa ugacagguaa aaguauacaa      960

cccgaaaauc uagaauauag aaucauguua ucaguacacg gaucacaaca uaguggaaug     1020

auaguaaaug auauaggaca cgaaaccgau gaaaauagag ccaaaguuga aguaacgcca     1080

aauucgccaa gagcugaagc aacauuagga ggauucggau cucuuggauu agauugcgag     1140

ccaagaacag gauuagacuu uagugauuua uacuaucuaa cuaugaauaa caaacauugg     1200

uuaguacaua aagaaugguu ucaugauauc ccauuaccuu ggcaugcagg agcagauaca     1260

gggacaccuc auuggaauaa uaaagaagca uuagucgagu ucaaagacgc ucaugcaaaa     1320

agacaaaccg uaguaguacu aggaucacaa gaaggagccg uacauacggc gcuugcagga     1380

gcauuagaag cagaaaugga uggagcaaaa ggaagauuau uuucuggaca uuuaaaaugu     1440

agauuaaaaa uggauaaacu gagauuaaaa ggaguuucuu auucuuuaug uacagcaguu     1500

uguacagcag cuaaaguccc agcagaaaca cuacauggaa caguaacagu agaaguacaa     1560

uaugcaggaa ccgauggucc auguaaagua ccagcucaaa uggcagucga uaugcaaaca     1620

uuaacuccgg uaggaagacu aauuacagcu aacccaguaa uaacagaguc aaccgaaaau     1680

agcaaaauga uguuagaauu agauccacca uuuggagaua gcuacauugu aauaggaguu     1740

ggagauaaga aaauaacaca ucauuggcau agaucgggaa gcaccauugg gaaagcauuc     1800

gaagcgacag ugaggggagc aaaaagaaug gcaguauuag gagauacagc gugggauuuu     1860

ggaucuguag gaggaguauu uaauaguuug ggaaaaggaa uacaucaaau auuuggagca     1920

gcauuuaaau cauuauuugg aggaaugagc ugguuuucac aaauauuaau aggcacacua     1980

uuggucuggc ucggacugaa uacuaaaaac ggaaguauua guuuaacgug ccuagcauua     2040

ggaggcguaa ugauauuccu uaguacagca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9313
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 3 opt

<400>  9313
gggagaaagc uuaccaugac cucaauagga auaguaggcu uguuguuaac aacagcaaug       60

gcagccgaaa uuacaagaag agguuccgca uauuauaugu accuagauag aucagaugca      120

gggaaagcaa uaaguuuugc aacaacuuua ggagucaaua aaugucaugu acaaauaaug      180

gauuugggac auauguguga ugcaacaaug agcuacgaau guccaauguu agaugaagga      240

guugaaccug acgauguaga uuguuggugu aauacaacaa guacaugggu aguauacgga      300

accugucauc auaaaaaagg agaagcucga agaagcagaa gggcuguaac acuaccgucu      360

cauagcacua gaaaauuaca aacacgaucc caaacauggc uagaaaguag agaauacaca      420

aaacauuuaa uaaaaguaga aaauuggaua uuuagaaauc ccggauucgc uuuaguagcc      480

guagcaauug caugguuauu aggaucaucu acaucacaaa aaguaauaua ucuuguaaug      540

auacuauuaa uagcgccggc auauaguauu agaugcauag gaguaaguaa uagagauuuu      600

guagaaggaa ugaguggugg uacgugggua gaugucguuc uagaacacgg aggaugcgua      660

acaguaaugg cacaagacaa accgaccgua gacauagaau uaguuacaac uaccguuuca      720

aacauggccg aagugagauc uuacuguuau gaagcuucaa uaagugauau ggcaucagau      780

ucaagaugcc cuacacaagg agaagcguac cuugacaaac aaucggauac acaauaugua      840

ugcaaaagaa cacuaguaga ucgaggaugg ggaaacgguu guggacuguu uggaaaagga      900

aguuugguaa cuugugcaaa auuuacaugu ucaaaaaaaa ugacagguaa gucaauacaa      960

ccagaaaacc uugaguauag aauaaugcua ucaguacaug gaagucagca cucaggaaug     1020

auaguaaaug acgaaaacag agccaaagua gaaguuacuc ccaauagucc acgagcggaa     1080

gcaacauugg gaggguuugg aucucuaggu cuagauugcg aaccgagaac cggauuagau     1140

uucagugauu uauauuauuu aacaaugaau aauaaacacu gguuaguuca caaagaaugg     1200

uuccacgaua ucccauuacc auggcaugcu ggagcggaua cgggcacacc acauuggaau     1260

aacaaagaag cacuaguuga auuuaaagau gcacaugcaa aacgucaaac aguaguagua     1320

uuaggauccc aagaaggcgc uguacauaca gcacuagcag gugcauuaga agcugaaaug     1380

gauggugcua aaggacgcuu auuuucagga caucuaaaau gucgucuaaa aauggacaaa     1440

uuaagacuua aaggaguaag uuauagcuua uguacugcag cauuuacuuu uacaaaagua     1500

ccagcugaaa cauuacacgg aacagugaca guugaaguac aauaugcugg gacagacgga     1560

ccauguaaag uaccagccca aauggcagua gauaugcaaa cauuaacgcc uguaggucgu     1620

uuaauaacug cuaacccugu cauaacggaa ucaacagaaa auucaaaaau gauguuagaa     1680

uuagauccac cuuucggaga cucauacaua guaauaggag uuggagacaa gaaaauaacu     1740

caccacuggc auagaucagg aaguacgauu gguaaagcau uugaagcaac ggugagagga     1800

gcaaaaagaa uggcaguauu aggggauaca gcgugggauu uuggaagugu aggaggaguu     1860

uuuaacucau uaggaaaagg uauacaccaa auauuuggug cagcauuuaa aucucuauuu     1920

ggagguaugu caugguuuuc acaaauauua auagguacac uauuaguaug guugggacua     1980

aauacaaaaa auggaucaau aucucuaaca ugcuuagcuu uaggaggagu aaugauauuu     2040

uuaaguacag cagugucagc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  9314
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 3 opt

<400>  9314
gggagaaagc uuaccaugac uucaauagga auaaucgggu uacuauuaac aaccgcaaug       60

gcagcugaaa uaacccgaag aggaucagca uacuauaugu accuagauag aucagacgca      120

ggaaaagcaa uaagcuuugc aacgacgcuc ggaguaaaca aaugucaugu ccaaauaaug      180

gauuugggac acauguguga cgcaacuaug ucauacgaau guccaaugcu ugaugaagga      240

guagaaccag augauguaga uuguuggugu aacacaacuu caacaugggu aguauacgga      300

acuugucauc auaagaaagg agaaaccaga cgaagccgaa ggucaguuag ucuaagauau      360

cacuacacaa gaaaacuaca aaccagauca caaacauggc uagaaucaag agaauauaaa      420

aaacacuuaa uuaugguaga gaauuggaua uuucguaauc cagguuuugc aauagugagu      480

guagcaauua cuuggcuuau gggaaguuua acuagucaaa aaguuauaua uuuaguaaug      540

auaguacuaa ucguaccagc auacagcaua ucaugcauag gaguuaguaa cagagaucua      600

guugaaggaa ugucaggagg aacuugggua gacguuguau uagaacacgg aggaugugua      660

acagaaaugg cgcaagacaa accgacaguc gauauagaau ugguaacaau gacaguaagc      720

aauauggcag aaguaagauc auauuguuau gaagcaucac uaucugacau ggcaucggca      780

ucacgaugcc caacacaagg ugaaccaagu uuagauaaac aaagugauac acaaagcguu      840

uguaaaagaa cauuagguga uagagguugg ggaaacgguu guggaauauu uggaaaagga      900

ucacucguaa cauguucuaa auuuaccugu uguaaaaaaa ugccaggaaa auccauucaa      960

cccgaaaauu uggaauauag aauaaugcug cccguacaug gaagucaaca uaguggaaug     1020

auaguaaaug auauaggaca ugaaacagau gaaaaucgug caaaaguuga aguaacacca     1080

aauucaccua gagcagaagc aacguuagga ggauuuggaa gucuaggcuu agauugugaa     1140

ccaagaacag gauuagauuu uuccgauuua uauuaucuaa caaugaauaa caaacacugg     1200

uuaguacaua aagaaugguu ucaugauaua ccauuaccau ggcaugcggg agcugacacu     1260

ggaacaccac auuggaacaa uaaggaagcg cuuguagaau ucaaagaugc acacgcaaaa     1320

agacaaacag uaguaguacu aggaucacaa gaaggagcag uacauacagc auuagccgga     1380

gcacuagaag cagaaaugga cggagcaaaa gguagacuau ucagugguca uuuaaaaugc     1440

agguuaaaaa uggauaaacu ccguuuaaaa ggaguuucuu auaguuuaug uacagcagca     1500

uuuacauuua cuaaaguacc ugcagaaaca uuacauggaa caguaacugu agaaguacaa     1560

ucagccggaa cagauggacc uugcaaagua ccugcacaaa uggcaguaga uaugcaaaca     1620

cuaacaccgg uaggaagacu aauaacagca aaccccguaa uaacugaauc aacagaaaac     1680

ucuaaaauga ugcuagaacu agauccuccu uuuggugacu cauauaucgu gauaggcgua     1740

ggagauaaaa aaaucacaca ucacuggcac agaucaggau cuaccauagg aaaagccuuc     1800

gaagcaacag uaagaggagc aaaaaggaug gcaguguuag gcgauacagc uugggauuuu     1860

ggcagcguag gcggaguauu uaacucacuu ggaaaaggaa uacaucaaau auuuggcgca     1920

gcauucaaau cccuauuugg aggaaugagu ugguuuucac aaauauuaau agguacauua     1980

cuuguauggu uagguuuaaa uacaaaaaac ggcucaauca guuuaacuug ccuagcuuua     2040

ggaggaguaa ugauauuucu aagcacagca gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9315
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 3 opt

<400>  9315
gggagaaagc uuaccaugag uguuggaaua guaggauuau uacuuacaac agcuauggca       60

gcagagguca caagacgagg aucagcuuac uauauguauu uagacaggaa ugaugcuggu      120

gaagcaauau cauucccgac cacccugggu augaacaaau guuacauaca aauuauggau      180

cuuggacaca ugugugacgc aacuauguca uaugaauguc caaugcuaga ugaaggagua      240

gaaccagacg auguagacug cuggugcaac acaacaagca cuuggguagu auauggcacc      300

ugucaucaua aaaaaggaga agcuagaaga aguagaagag caguaacauu accaagccau      360

uccacuagaa aauuacaaac aagaucacaa acaugguuag aauccaggga auauacaaag      420

caccucauaa gagucgaaaa uuggauauuu agaaacccag gauucgcccu ugcagccgca      480

gcaauagccu gguuacuugg aucgagcaca agucaaaaag uuaucuaucu cguaaugaua      540

uuauuaauag caccagcaua uagcauacga uguauuggcg uaucgaauag agauuuugua      600

gaaggaauga gcggaggaac auggguagac gucguucuag aacauggugg augcguaaca      660

guaauggcac aagauaaacc uacaguugau auagaauuag uaacaacaac aguaaguaau      720

auggcagaag uaaggagcua uugcuacgaa gcaucaauca gugauauggc auccgauuca      780

agauguccaa cacaaggaga agcauacuua gauaaacaau cagauacaca auauguaugu      840

aaacguacac uuguagacag aggaugggga aacggaugug gucuauucgg aaaggguagc      900

cuaguaacau gcgcaaaauu cgcaugcagu aaaaaaauga cugguaaaag cauacaacca      960

gaaaaucuug aauauagaau aauguuauca guacauggaa gucaacauuc ugggaugaua     1020

guuaaugaua ccggacauga aacugaugaa aacagagcaa aaguugaaau uacuccaaau     1080

ucaccaagag cagaagcaac auuagguggc uuuggaaguu ugggacuuga uugcgaaccu     1140

agaacaggac uagacuuuag ugauuuguau uacuuaacca ugaacaauaa acauugguua     1200

guacauaaag aaugguucca ugacauucca uuacccuggc augcuggagc ggauaccggg     1260

acaccacauu ggaacaacaa agaagcauua guagaauuua aagacgcaca cgccaaaaga     1320

caaacuguag uaguacuggg aagccaagaa ggagcaguac auacagcgcu agcaggagca     1380

uuagaagcag aaauggaugg ugcaaaagga agauuaagcu caggucauuu aaaaugcaga     1440

cuaaaaaugg acaaacuuag auuaaaaggu guaaguuauu cacuauguac ugcagcuuuc     1500

acauuuacua aaauaccagc agaaacacua cauggcacag uuacuguaga aguccaauac     1560

gcaggaacag auggaccuug uaaaguuccc gcacagaugg ccguagauau gcaaacacuu     1620

acuccaguag gccgacuuau aacagcaaac ccuguaauaa cagaaucaac agaaaauucc     1680

aaaaugaugu uggaauuaga ccccccguuu ggugauaguu acauaguaau aggagucggg     1740

gaaaaaaaaa uaacccauca cuggcaucga uccggcagca caauaggaaa agcauuugaa     1800

gccacuguaa gaggagcaaa aagaauggcg guauuaggag auacagcaug ggauuuugga     1860

agcguaggug gcgcacuaaa uuccuuagga aaagguauac aucaaauauu uggagcugca     1920

uuuaaaucuc uuuuuggggg aaugucaugg uuuagucaaa uuuuaauagg aacucuauua     1980

augugguuag gucucaauac aaaaaacgga ucaauaaguu uaauguguuu agcuuuggga     2040

ggaguccuaa uauuuuuaag cacggcugua agcgcaugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  9316
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 3 opt

<400>  9316
gggagaaagc uuaccaugac aucaguaggu auaguaggac uacuguuaac aacagcaaug       60

gcagcagaag uaacuagaag aggaucggca uauuauaugu aucuugaccg gaaugaugcg      120

ggagaagcua uaagcuuucc aacuacuuua ggcaugaaua aauguuauau ccaaauaaug      180

gaucuaggac auauguguga cgcaacaaug aguuacgaau guccaauguu agacgaaggg      240

guagagccug augacguaga uugcuggugc aacacgacaa guacaugggu cgucuaugga      300

acaugucauc auaaaaaagg agaagcacga agaaguaggc gagcaguaac ucuucccuca      360

cacaguacac gaaaauuaca aacaagaagu caaacauggu uagaaucccg agaguauaca      420

aaacacuuga uaagaguaga aaauuggauu uuuagaaauc caggauuugc acuagcagcc      480

gcagcaaucg cauggcuacu uggaagcagu acaagucaaa aagucauuua uuuagucaug      540

auauuauuaa uugcaccugc auauucaaua agaugcaucg gagugucgaa uagagauuuu      600

guagaaggaa ugaguggagg aacaugggua gauguaguau uagaacaugg aggcugugua      660

acugucaugg cccaagauaa acccacagua gauauagaau uaguaacgac aacaguauca      720

aauauggcag aaguaagauc auacuguuau gaggcuagua uuucagacau ggcaagugau      780

ucuagauguc ccacgcaagg ugaagcauau cuagacaagc aaucagauac acaauaugua      840

uguaaaagga cauuagugga ucgaggcugg ggaaaugguu guggacuuuu ugggaaaggu      900

ucauuaguca caugugcaaa auuugcgugu ucaaaaaaaa ugacaggaaa aaguauccaa      960

ccagaaaacu uagaauacag aauaaugcua ucuguacacg gaucucaaca uaguggcaug     1020

auaguaaaug auacgggaca ugaaacugac gaaaacagag caaaaguaga aauaacacca     1080

aacucaccua gagccgaagc aacauuaggc ggauuuggau cucuaggauu agauugcgaa     1140

ccaagaacug gauuagauuu cucagauuua uauuaucuaa caaugaauaa caaacauugg     1200

uugguacaua aagaaugguu ucacgacauc ccauuaccau ggcaugcagg agccgauacu     1260

ggaacaccac auuggaacaa uaaagaagca cuuguugaau uuaaagaugc acacgcaaaa     1320

cgacaaacug uuguaguucu aggaucgcaa gaaggugccg uacauacagc gcuugcagga     1380

gcacucgaag cagaaaugga uggcgcaaaa ggaagauuaa guucagggca ucuuaaaugc     1440

agacuaaaaa uggauaaauu aagguuaaaa ggaguuucau auaguuuaug cacagcagca     1500

uuuacauuua cuaaaauacc agcggaaaca uugcacggaa cagugaccgu agaagugcaa     1560

uaugccggaa cagacggucc cuguaaagua ccagcucaaa uggcuguaga caugcaaacg     1620

uuaacuccag ucggaagauu aaucacagcg aacccaguaa uaacagaaag uacagaaaau     1680

ucaaaaauga uguuagaauu agacccacca uuuggugaua gcuauauagu aauaggagua     1740

ggugaaaaaa aaauaaccca ucauuggcau aggucuggaa guacaauagg uaaagcauuc     1800

gaagcaacag uaagaggagc uagaagaaug gcuguauugg gagauacugc gugggacuuu     1860

ggaucaguag ggggagcccu uaauucacua ggaaaaggaa uacaucaaau uuuuggagca     1920

gcauucaaau cacuauuugg agguauguca ugguuuagcc aaauucuaau aggaacauua     1980

uuaauguggc uuggauuaaa cacaaaaaau ggaaguauuu cacuaaugug ccuagcacuc     2040

gguggagugu uaaucuuuuu aucaacagca guaucugcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9317
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 3 opt

<400>  9317
gggagaaagc uuaccaugac uucaguaggu auaguaggau uacuuuuaac uaccgcgaug       60

gcagcagaag uaacaagaag agggucagcu uauuacaugu acuuagacag aaaugaugca      120

ggagaagcaa ucaguuuccc aacuacauua gguaugaaca aauguuacau ccaaauuaug      180

gauuuaggac auauguguga cgcaacaaug uccuacgaau guccaauguu agaugaagga      240

guugaacccg augacgucga cuguuggugc aauacaacaa guacuugggu agucuauggg      300

acaugccauc auaagaaagg agaagcgaga agaagucgac gagcaguaac acuaccaucu      360

cauaguacaa gaaaauuaca aacacgaagu caaacauggc uagaaucuag agaauauaca      420

aaacaucuaa ucagaguaga aaauuggaug uuucgaaauc cuggauuugc acuagccgca      480

gcagcaauag cauggcuauu aggaucauca acaucacaaa aaguaauuua cuuaguaaug      540

auuuuguuaa uagcuccugc auauaguaua agaugcauag gaguaucaaa cagagauuuu      600

guagaaggaa ugucaggagg uacaugggua gauguaguuu uagaacaugg cggaugugua      660

acaguuaugg cacaagauaa accaacugua gauauagaau uaguaacaac uacaguaucu      720

aacauggcug aaguuagauc guacuguuac gaagcaagca uuucugacau ggcaucagau      780

ucuagauguc cuacacaagg agaagcauau cuugauaaac agucagauac acaauacgua      840

uguaaaagaa cauuaguaga uaggggaugg ggaaauggau gcggucuauu ugggaaagga      900

ucguuaguua caugugcaaa auucgcaugu aguaagaaaa ugacaggcaa aucaauacaa      960

cccgagaacc uugaauauag aauaauguua agcguucaug gaucccaaca cagcggaaug     1020

auagucaacg auaccgguca cgaaacugau gaaaaccgug caaaaguaga gauaacucca     1080

aacucaccac gugcagaagc aacauuagga gguuuuggcu cucuaggacu cgacugugaa     1140

cccagaacug gucuagacuu uucagaucua uauuauuuga caaugaauaa caaacauugg     1200

cugguucaua aagaaugguu ccaugauaua ccauuaccau ggcaugccgg agcagauaca     1260

ggaacuccgc acuggaacaa uaaagaggcu uuaguagaau uuaaagaugc ccaugcaaaa     1320

cgacaaacag uaguuguauu aggaagucaa gaaggugcag uucacacagc ucuggcagga     1380

gcauuagagg cagaaaugga cggcgcuaaa gguagauuau cauccggaca uuuaaaaugu     1440

agauuaaaaa uggauaaauu aagauuaaaa gguguaagcu auagccuaug cacugcugca     1500

uuuacauuca caaaaauacc agcagaaaca uuacauggaa caguuacggu ugaaguucaa     1560

uaugcaggua cugacggacc auguaaggua ccugcacaaa uggcagugga uaugcaaaca     1620

uugacucccg uugguagguu aauaacagca aauccaguca uaacugaauc aacagaaaau     1680

ucaaaaauga ugcuagaauu agauccucca uuuggagaca guuacauagu aauaggagua     1740

ggugaaaaga aaauaacaca ucauuggcac agaagcggaa guacuaucgg aaaagccuuu     1800

gaagccacgg uuagaggugc aaaaagaaug gcgguauugg gagauacagc augggacuuc     1860

ggcaguguag gaggagcuuu aaauucauua ggaaaaggaa uacaccaaau auucggagcu     1920

gcauuuaaaa gccucuucgg agguauguca ugguuuuccc aaauucuaau aggaacacua     1980

cuaauguggu uaggacuaaa uacuaaaaau ggaucaauau cacuaaugug cuuagcacuc     2040

ggaggaguac uaaucuuccu aucaacagca guaagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9318
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 3 opt

<400>  9318
gggagaaagc uuaccaugac aaguguaggu auaguaggac uauuauuaac aacagcuaug       60

gcagcagaag uaacgcggag agggucagcg uauuauaugu aucucgauag aaacgaugcc      120

ggagaagcaa uaagcuuucc aacaacauug ggaaugaaua aauguuauau acaaauaaug      180

gauuuaggac auauguguga ugcaaccaug ucauacgagu gcccaauguu agaugaaggu      240

guagaaccag augauguuga uuguuggugc aacaugacaa guacaugggu agucuaugga      300

acaugucauc acaaaaaagg agaagcaaga agaucaagaa gagcaguaac acuacccucc      360

cauucaacga gaaaacuuca aacaaggagu caaacauggu uagaaucccg agaauacaca      420

aaacaucuaa uaagaguaga aaauuggaua uuucgaaauc cagguuuugc ucuagcugca      480

gcagcgauag caugguuacu uggaagcucu accucacaaa aaguaauaua uuuaguuaug      540

auucuacuaa uagcaccagc auauucaaua agaugcauag gaguaucaaa ucgagauuuu      600

gucgaaggaa ugucaggagg aacaugggua gauguugucc uagaacaugg aggguguguu      660

acuguaaugg cacaagauaa accuacagua gacauagaac uuguaacaac aacaguauca      720

aauauggcug aaguucguuc auauuguuau gaagcuagua uaagcgacau ggccagcgau      780

ucaagauguc caacacaagg agaagcauac uuagauaaac aaagugauac acaauaugua      840

ugcaaacgaa cucuuguuga uagaggaugg gguaaugguu gcggauuauu uggaaaagga      900

ucacuuguaa caugugcaaa auuugcaugu aguaagaaaa ugacgggcaa aucaauacaa      960

cccgaaaauu uggaauauag aauaauguua aguguucaug gaagccaaca cucaggaaug     1020

auaguaaaug auacaggaca ugaaacagau gaaaaucgag caaaaguaga aauaaccccu     1080

aacagcccac gagcagaagc aacguuagga ggauuuggau cacuaggccu agauugugaa     1140

ccacgaacug gacuugacuu uagcgauuua uacuaccuga cuaugaacaa caaacacugg     1200

uuaguacaua aagaaugguu ucaugacaua ccacuuccuu ggcaugcagg ugcagauaca     1260

ggaacaccac auuggaauaa uaaagaagcu uuaguagaau uuaaagaugc gcaugcaaaa     1320

cgccaaacag uuguaguauu aggcucccaa gaaggagcgg uacauaccgc ccucgcagga     1380

gcauuagaag cagaaaugga uggagccaaa gguagauuau caucuggcca uuuaaaaugu     1440

agguuaaaaa uggacaaauu acgauuaaaa gguguaaguu auucauuaug cacagcugca     1500

uucacauuca caaaaauucc agcagaaaca uuacacggaa caguaacagu ugaaguucaa     1560

uacgcgggaa ccgacggacc auguaaggua ccagcccaaa uggccguaga caugcaaaca     1620

uuaacaccag uaggaagauu aauaacagcu aauccaguaa uaaccgaaag cacagaaaau     1680

ucaaaaauga uguuagaauu agauccucca uuuggagauu cguauaucgu gauuggggua     1740

ggagaaaaaa aaauuacaca ccacuggcau agaucaggcu caaccauagg aaaagcauuu     1800

gaagcuacug uaagaggugc uaaaagaaug gcaguucucg gugacaccgc augggacuuu     1860

ggaaguguag guggugcauu aaauucauua ggaaaaggaa uccaccaaau auuuggagca     1920

gcauuuaaau cguuauucgg aggcauguca ugguuuaguc aaauauuaau agguacuuua     1980

uuaauguggu uaggacuaaa uacaaaaaau gguaguauaa guuuaaugug uuuagcucua     2040

ggaggaguau ugauauucuu aucaacugca guuagcgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9319
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 3 opt

<400>  9319
gggagaaagc uuaccaugac cagcguagga auaguugguc uauuacuaac aacagcuaug       60

gcagcagaag uaacaagaag gggaucagca uauuauaugu auuuagaucg aaaugaugcu      120

ggagaagcca uaucuuuucc cacaacacua ggaaugaaca aaugcuauau acaaauuaug      180

gauuuagguc auauguguga ugcaacaaug ucauacgaau guccuauguu agaugaagga      240

guagaaccgg augauguaga uuguuggugu aauacaaccu caacaugggu agucuacggu      300

acuugucauc auaaaaaagg ugaagcaaga aggaguagga gagcgguaac uuuaccaucg      360

cauucaacaa ggaaacuuca aacaagaagu caaacauggc uagaaaguag agaauauaca      420

aaacaucuca uaagaguuga aaacuggaua uucagaaauc cuggcuuugc uuuggcugca      480

gcagcaauag cgugguuacu agggucauca acaucgcaaa aaguuauaua ccugguuaug      540

auauuauuaa uagcaccggc auauaguauu agauguauag gaguaucaaa uagagauuuu      600

guugaaggaa ugagcggcgg aacaugggua gacguuguuc uagaacaugg aggaugcgua      660

acagcuaugg cucaagauaa accgacagua gacauagaau uaguaacaac uacaguuagc      720

aauauggcug aagucagguc auacuguuac gaagccagca uaagugauau ggcuagcgac      780

aguagauguc ccacucaagg agaagcauau cuagauaaac aaagcgauac acaauaugug      840

uguaaacgua cauuaguaga cagaggaugg ggcaauggau gcggauuauu uggaaaaggu      900

agucuaguaa caugugcaaa auuugccugc agcaaaaaaa ugacaggaaa aaguauacaa      960

ccagaaaacu uagaauaccg aauaauguua ucaguacaug guagucaaca caguggaaug     1020

uuaguaaacg auacagguca ugaaacagau gaaaaucgug caaaaguaga aauaacacca     1080

aauucgccaa gggcagaagc aacucuagga ggauuuggau cacuagguuu ggauugugaa     1140

ccaaggacag gacuagauuu uucagauuua uauuaucuaa cuaugaauaa uaaacauugg     1200

uuagcacaua aagaaugguu ucacgauaua ccauuaccau ggcacgcggg agcagcaacu     1260

ggcacaccac auuggaauaa uaaagaagca uuaguagaau ucaaagaugc acacgcaaaa     1320

aggcaaacug uaguaguacu aggcucacaa gaaggagccg uacauaccgc cuuagcagga     1380

gcauuagaag cugaaaugga uggagcaaaa ggaagauuau cuucuggaca ucuaaaaugu     1440

cgauuaaaaa uggauaaacu aagauugaaa ggaguaagcu acuccuugug uacagcugca     1500

uuuacauuua caaagauccc agcugaaaca guugauggaa cuguaacugu agaaggacag     1560

uauggaggaa cagaugggcc uuguaaaguu ccagcacaaa uggccguaga uaugcaaacg     1620

uuaacuccug uuggaagacu aauaacagca aauccgguaa ucacagaaag cacagaaaau     1680

agcaaaauga uguuagaauu agacccaccu uuuggagauu cauauauagu aauuggugua     1740

ggagaaaaaa aaauaacaca ccacuggcau agaucuggaa guacaauagg aaaagcauuu     1800

gaagcaaccg uaaggggagc gaaaagaaug gcaguucuag gcgauacagc cugggacuuu     1860

ggaagcgucg gaggcgcauu aaauaguuua ggaaaaggaa uacaucaaau uauaggagcu     1920

gccuucaaau cauuauucgg aggcaugucu ugguuuaguc agauauuaau cgguacauua     1980

cuaauguggu uaggauuaaa uacaaaaaac gguagcauau cauuaaugug ucuagcguua     2040

ggaggaguuu uaauuuuucu aaguacagca guaucagggu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9320
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 3 opt

<400>  9320
gggagaaagc uuaccauggg ggcagacgcu ucuauaggaa uaguaagccu auuauuaacu       60

acuguuaugg cagcagaaau aacaagaagg gguagugccu auuauaugua uuuagacaga      120

agugaugcag gaaaagcaau aaguuuuguc acaacuuuag gaguaaauaa augucacguu      180

caaauaaugg auuuaggaca uaugugcgau gcgacaauga guuaugaaug uccuauguua      240

gaugaaggcg uagaaccaga ugacguagau uguuggugua auaccacauc aacuuggguu      300

guauauggaa cuugucauca caaaaaagga gaagcgagaa gaucaaggag agcaguaaca      360

uuaccaaguc auucaacaag aaaacuucaa acaagaagcc aaacaugguu agaaaguaga      420

gaguacacua aacaucuaau aaaaguagaa aauuggauau uuagaaaucc agguuuugca      480

cuaguugcag ucgcuauagc cuggcuacua ggaucaucua caagccaaaa aguaaucuau      540

cuaguaauga ucuuacuaau agcaccagca uauaguauaa gauguauugg aguaagcaau      600

agagauuuug uagaaggaau gagcggagga acuuggguug acguaguauu agaacauggc      660

gguugcguua cagucauggc acaagauaaa ccaacaguag auauugaacu agugacaaca      720

acagugagca auauggcaga aguaagaucg uauuguuaug aagcaucaau aucugauaug      780

gcuucugaua gcagaugccc cacacaaggu gaagcauauu uagacaaaca aagugacaca      840

caauauguau guaaaagaac auuaguagac agaggauggg gaaacggaug uggauuguuu      900

ggaaaaggua guuuaguuac augugcaaaa uucacuuguu caaaaaaaau gacaggaaaa      960

ucuauccaac cagaaaauuu agaauacaga auuaugcuaa guguucaugg aagucaacac     1020

ucgggaauga uagucaauga cgaaaacagg gccaaaguug aaguuacgcc aaacucaccu     1080

agagcagaag ccacacuagg aggauuugga ucacuaggac uugacuguga acccagaacg     1140

ggacuagauu uuagugauuu auauuaucuu acaaugaaua acaaacauug gcuaguacac     1200

aaagaauggu uucaugacau accacuaccc uggcacagug gugcagauac cgaaacacca     1260

cauuggaaca auaaagaagc acuaguagaa uucaaagaug cacaugcaaa gagacagacc     1320

gugguuguac uagggagcca ggaaggagca guacacacgg cauuagcggg agcauuagaa     1380

gcagaaaugg acggagcgaa aggaagauua ucaagcggac aucuaaaaug uagauuaaaa     1440

auggauaaau ugagauuaaa aggaguuuca uacaguuuau guaccgcugc auuuacuuuu     1500

acuaaaguuc ccgcugaaac gcuacacgga acaguaacag uagaaguuca auaugccgga     1560

agagacgguc ccuguaaagu acccgcacaa auggccguag auaugcaaac acuaacaccg     1620

gugggaagac uaauaaccgc caacccaguu auuacagaau caacagagaa uucaaaaaug     1680

auguuagaau uagauccccc auuuggugau ucuuauauag uaauaggagu aggugauaaa     1740

aaaauaaccc accacuggca uaggagcgga ucaauaauag gcaaagcauu cgaagcaaca     1800

guccgaggag caaaaagaau ggcaguacuu ggugauaccg caugggacuu uggaagugua     1860

ggaggaguau uuaauagucu agguaaagga auucaucaaa uuuuuggagc ggcuuuuaag     1920

ucauuauucg gaggaauguc cugguucagu caaauccuaa uaggaaccuu gcuaguaugg     1980

uuaggauuaa auacaaaaaa uggcucaauu ucauuaacau gucuagcacu uggcggagua     2040

augauauuuu uauccacugc aguaucggca ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  9321
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 3 opt

<400>  9321
gggagaaagc uuaccauggg agccgacacu aguauaggaa uaauaggauu auuauuaacu       60

acagcaaugg cggcugaaau aacucgaaga ggaucagcau auuauaugua uuuagauaga      120

ucagaugcug gaaaagcaau aaguuuugca acaacaaugg gaguaaacaa augccaugua      180

cagauuaugg aucuagguca caugugugau gcuacaauga guuaugaaug cccaauguua      240

gaugagggag uagaaccaga ugauguggau uguuggugua auacaacguc aacauggguu      300

guauauggaa caugucacca caaaaaagga gaagcaaggc cgucaagacg agcaguaaca      360

uuaccuaguc auaguaccag aaaacuacaa acuagaagcc aaacaugguu agaaaguaga      420

gaauauacaa aacacuuaau aaaaguggaa aauuggauac uaaggaaucc aggcuuuacu      480

guagcggcau uagcuauggc auggcuacuu gggaauucaa caucacaacg aguaauauau      540

uuaguaauga uauuacuaau agcgccugca uauuccauaa gaugcauugg aguaagcaac      600

cgcgacuuug uagagggaau gucaggcgga acuuggguug auguaguauu agaacacggu      660

ggauguguaa cuguaauggc acaagacaaa ccuacaguag acauugaauu aguuacgaca      720

acaguaagca auauggcaga aguacguucc uauuguuaug aagcuaguau aucagauaug      780

gcaagcgauu cuagaugucc uacacaggga gaagcauauu uagacaaaca aagugauaca      840

caauacguau gcaaacgcac ccuaguagau cgaggauggg gaaacgggug cggauuauuu      900

gguaaaggau cauugguuac uugcgcgaaa uuuacaugua guaaaaaaau gacaggaaaa      960

ucaauucaac cugaaaauuu agaauaucga auaaugcugu caguacaugg aucacaacau     1020

aguggcauga uagucaauga uauaggacau gaaacagaug aaaacagggc caaaguagaa     1080

gucaccccca auucccccag agcagaagcg acauuaggcg gcuuuggaag uuuaggauua     1140

gauugcgaac ccagaacagg auuagauuuu ucugauuuau auuaucuaac aaugaacaac     1200

aaacacuggu uaguacacaa agaaugguuu cacgacauac cccuaccgug gcaugcagga     1260

gcagauaccg gaacaccaca uuggaauaau aaagaagcau uaguagaauu uaaagaugcu     1320

cacgcaaaaa gacaaacagu uguaguauua ggaucucaag aaggugcugu acauacagca     1380

cuugcaggag cauuagaagc agaaauggau ggagcaaaag gaagauuauu uuccggacac     1440

cuuaaaugca gaauaaaaau ggacaagcua agauuaaagg gaguaaguua uucacuaugu     1500

acugcagcuu uuacauuuag uaaaguacca gcagaaacuu uacaugguac cguaacuguu     1560

gaaguucaau augcaggaag ggaugggcca ugcaaaguac cugcccaaau ggcaguagau     1620

augcaaacuu uaacgccugu agguagauua auuaccgcaa auccuauuau uacagaauca     1680

acugaaaaua gccgcaugau guuagaacua gauccgccau uuggugauag cuauauaguu     1740

auaggaguag gagauaaaaa aauaacacau cauuggcaua gaaguggauc aaccauaggc     1800

aaagcauuug aagcaacagu aagaggagcg aaaagaaugg caguacuagg cgauacugca     1860

ugggacuuug gaucaguagg uggaguauuc aauucauuag gaaaaggagu acaucaaguc     1920

uuuggagcug cuuucaaaag uuuauuugga gggaugucau gguuuagcca aauauuaauu     1980

gggacuuuau uaguuuggcu gggauuaaau acaaaaaaug gaaguauauc auuagcuugu     2040

cuugcacuag gaggagcaau gauauuccua agcacagcug uaucagcaug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  9322
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 3 opt

<400>  9322
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauacu auuaauagca       60

ccagcuuaua gcauaagaug uauaggaguu ucaaacaggg auuucgucga aggaaugagu      120

gguggaacau ggguagauau aguucuagaa cauggagguu guguaacagu gauggcacaa      180

gauaaaccaa cuguagauau agaauuaguu acuacaaccg uaucaaauau ggcagaaguu      240

agaucauauu guuaugaagc aucaauaagu gauauggccu cagauucaag auguccaaca      300

cagggggaag ccuacuuaga uaaacaauca gauacucaau auguauguaa aagaacauua      360

guagauagag guuggggaaa uggaugugga uuauuuggaa aagguaguuu aguaacgugu      420

gcaaaauucg cauguucuaa aaaaaugaca ggaaaaagua uacaaccuga aaaccuagaa      480

uauagaauaa ugcuaucagu acaugguagu caacauucgg gaaugauagu aaaugauaca      540

ggacaugaaa cagacgaaaa uagagcaaaa guugaaauaa ccccaaauag uccaagagca      600

gaagcaacau uaggaggauu uggaucauua ggacuagauu gcgaaccaag aacaggauua      660

gauuuuagug auuuauauua ucuaacaaug aauaauaaac acugguuagu acauaaagaa      720

ugguuccaug auauaccacu accauggcac gcaggugcag auacaggaac accacauugg      780

aauaauaaag aagcauuagu ugaauucaaa gacgcccaug caaaaagaca aacaguagua      840

guacuuggua gucaagaagg ugcuguucac acagcauugg caggagcauu agaagcagaa      900

auggauggag cuaaaggaag auuaucauca ggacauuuaa aaugcagauu aaaaauggau      960

aaacuaagac uaaaaggagu aaguuauagu cuaugcacgg cggcauuuac auucacuaaa     1020

auaccagcgg aaacacuaca ugggacagua acaguagaag uacaauaugc aggaacagau     1080

ggaccuugua aaguuccagc ucaaauggca guagauaugc aaacauuaac accgguaggu     1140

agauuaauaa cagcuaaccc aguaauaaca gaaaguacug aaaauucaaa aaugaugcua     1200

gaacuagauc caccauuugg agacucauau auaguaauag gaguaggaga aaaaaaaaua     1260

acucaucauu ggcacagauc aggaaguaca auaggaaagg cauucgaagc aacaguaaga     1320

ggagcuaaaa gaauggcagu uuuaggagau acagcauggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9323
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 3 opt

<400>  9323
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauacu acucauagca       60

ccagcuuaua guauaagaug cauuggagua ucaaaucgag auuuuguaga agguauguca      120

ggaggaacau ggguagaugu aguacuagaa caugguggau guguaacugu aauggcacaa      180

gacaaaccaa caguagacau agaauuggua acaacaacag uaaguaauau ggcagaagua      240

agaucauauu guuaugaagc aagcauauca gauauggcaa gugacagcag guguccaacu      300

caaggugaag cauaccuaga uaaacaauca gauacacaau auguuuguaa aagaacauua      360

gucgacagag guuggggaaa ugguugcgga cuauucggaa aaggaagcuu aguaacaugu      420

gcaaaauuug cauguagcaa aaaaaugaca gguaaaucaa uucaaccaga aaauuuagaa      480

uacagaauaa ugcuuagugu acacgguucc caacauagug gaaugaucgu aaaugacacc      540

ggacaugaaa cugaugaaaa ucgugcaaaa guagaaauaa caccaaauuc cccaagagcu      600

gaagcaacac uaggaggauu cgguucauua ggauuagauu gugaaccuag aacaggauua      660

gauuuuucag aucuauauua ucuaacgaug aauaauaaac auugguuagu acauaaagaa      720

ugguuucaug auauaccauu acccuggcau gcuggagcag auacagggac accacauugg      780

aauaauaaag aagcguuagu ugaauuuaaa gacgcacaug caaaaagaca aacaguagua      840

guacuuggau cacaagaagg cgcaguacau acagcacuag caggagcacu ggaagcugaa      900

auggauggug caaaggguag acuaucaagc ggacaucuaa aauguagacu aaaaauggau      960

aaacuaagac uaaaaggagu uuccuauagu uuguguacag cagcauuuac uuuuacaaaa     1020

auaccagcag aaacauuaca uggaacagua acaguagaag uucaauaugc aggaacggau     1080

ggaccaugua aaguaccagc acaaauggca guagacaugc aaacauuaac accaguagga     1140

agauuaauaa ccgcuaaucc aguaauaaca gaaucaacag aaaauucaaa aaugaugcuu     1200

gaacuugauc caccauuugg agauucauac auaguaauag gaguaggaga aaaaaaaaua     1260

acacaucacu ggcacagaag uggaucaaca auaggaaaag cauuugaagc aacgguaaga     1320

ggagcaaaaa gaauggcagu auuaggagau acugccuggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9324
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 3 opt

<400>  9324
gggagaaagc uuaccauguc ucaaaaagua auauauuuag uuaugauauu auuaauagca       60

ccagcauauu caauaagaug uauaggagua ucaaauagag acuuuguaga aggaaugagu      120

ggaggaacau ggguagaugu aguacuagaa cauggaggau guguaacagu uauggcacaa      180

gauaaaccaa cgguagacau agaauuagua acaacuacag uaucaaauau ggcagaagua      240

agaagcuacu guuaugaagc aucaauuagu gacauggcaa gugacagcag auguccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacacaau acguaugcaa aagaacauua      360

guagacagag gauggggaaa uggaugugga uuguuuggca aaggaaguuu aguaacaugu      420

gcgaaauuua cuuguaguaa gaaaaugaca gggaaaucaa uacaaccaga aaauuuagaa      480

uauagaauaa ugcuaagugu acaugguuca caacacucag ggaugauugg uuacgaaacg      540

gaugaagaua gagcaaaagu agaaguuaca ccaaauagcc ccagagcaga agcaacguua      600

gguggcuuug gcaguuuagg auuagauugu gaaccaagaa caggauuaga cuuuagugau      660

cuauauuauu uaacaaugaa uaauaaacau ugguuaguac acaaagaaug guuccaugau      720

auaccucuac cauggcaugc aggagcagau acaggcacac cgcacuggaa uaauaaagaa      780

gcauuaguag aauuuaaaga ugcacaugca aaacgacaaa ccguagucgu auuaggaagu      840

caagaagggg caguucacac agcauuagca ggagcucuug aagcagaaau ggauggagca      900

aaaggaagau uauucucagg acacuuaaaa uguagacuaa aaauggauaa auugagacuc      960

aaagguguau cauauagucu guguacagca gcauucacau uuacaaaagu accugcagaa     1020

acauuacaug ggacaguaac aguugaagua caauaugcag guacugaugg accauguaaa     1080

aucccuguac aaauggcagu ggauaugcaa acucuaacac caguaggaag acuaauaaca     1140

gcuaauccag uaauaacaga aagcacagaa aauagcaaaa ugauguuaga auuagaucca     1200

cccuucggag auaguuacau aguaauagga guuggagaua agaaaauaac acaccacugg     1260

cauagaucag gcaguacaau aggaaaagca uuugaagcua cagugagagg ugcuaaacga     1320

auggcuguau uaggagauac agcuugggau uuuggaaguu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  9325
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 3 opt

<400>  9325
gggagaaagc uuaccaugag ccaaaaaguu auauaucuag uuaugauacu auuaauagcc       60

ccagcauaua guauaaggug uauaggggug aguaacagag auuucguaga aggaauguca      120

ggaggaacau ggguagaugu aguacuagaa cacggaggau guguuacagu aauggcacaa      180

gauaaaccaa caguagauau agagcuagua acgacaacag uaaguaauau ggcagaaguu      240

cgaucuuauu guuaugaagc aaguauaagu gauauggcaa gugauucacg uuguccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacucaau auguuugcaa aagaacauua      360

guagauagag gauggggaaa cggguguggu uuauuuggua aaggaaguuu aguaacaugc      420

gcaaaauuug cauguagcaa gaaaaugacu ggaaaaagua uacaaccaga aaauuuagaa      480

uauagaauaa ugcuaagcgu acacgguagu caacauagug guaugauugu caaugauacu      540

ggacaugaaa cagacgaaaa uagagcuaaa guagaaauaa caccaaauag cccacgagca      600

gaagcuacac ucggaggauu ugguucuuua ggauuggauu gugaaccucg aaccggacua      660

gauuuuagcg aucucuacua cuuaacaaug aauaauaaac auuggcuagu acauaaagaa      720

ugguuucaug auaucccauu accauggcau gcaggagcag auacaggaac accacauugg      780

aacaauaaag aagcauuggu agaauuuaaa gacgcacaug caaaaagaca aacuguagua      840

guacuaggau cacaagaggg ugcuguacau acagcgcuag caggcgcacu agaagcagaa      900

auggauggug caaaagggcg auuaaguuca ggacaucuaa aauguagauu aaaaauggau      960

aaauuaagac uaaaaggagu aucauauuca uuauguacug cugcauuuac auuuacaaaa     1020

auaccggcag aaacauuaca uggaacagua acaguagaag uacaauaugc aggaacagac     1080

ggaccaugua aagucccagc acaaauggca guugauaugc aaacauuaac accuguagga     1140

agauuaauaa cagcaaaucc aguaauaaca gaaucaacag aaaacucaaa aaugauguua     1200

gaauuagauc cuccauuugg ugacagcuau auaguaauag guguaggaga aaagaaaauu     1260

acacaccauu ggcauagaag cggaucaacc auaggaaaag cauuugaggc aacaguaaga     1320

ggagcaaaaa gaauggcagu auugggagac acagcauggg auuucggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9326
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 3 opt

<400>  9326
gggagaaagc uuaccaugag ccaaaaagua auauauuuag uaaugauauu acuuauagcu       60

ccagcuuaua guauaagaug uauagggguc ucaaauagag acuuuguuga aggaaugagu      120

ggaggaacau ggguagacgu aguuuuagaa cauggaggau guguaacugu aauggcacaa      180

gauaaaccga caguagauau agaauuagua acaacaaccg uaaguaauau ggcagaagua      240

agaagcuauu gcuaugaagc aagcaucagu gauauggcaa gugauaguag auguccaaca      300

caaggagaag cauaccuaga caaacaaagu gacacacaau auguauguaa aagaacacua      360

guagauagag gauggggaaa cggaugcgga uuauuuggua aagguucauu aguaacaugu      420

gcaaaauuua cauguagcaa aaaaaugaca ggaaaaagca uucaaccaga aaaucuagaa      480

uacagaauca ugcuaucugu acauggauca caacacagug guaugauagu uaaugauacu      540

ggauaugaaa cagaugaaaa uagggcaaaa guggaaguaa cuccaaauag cccaagagca      600

gaagcuacau uaggaggauu cggaaguuua ggacuagauu gcgaaccaag aacaggacua      660

gacuuuucag aucuauauua uuuaacuaug aauaauaaac auugguuagu acacaaagaa      720

ugguuucaug auauaccauu accauggcac gcaggagcag auacuggaac accccauugg      780

aauaauaaag aagcacuagu agaauucaaa gaugcucaug caaaaagaca aacuguagua      840

guauuaggcu cacaagaagg agccgugcau accgcauuag cuggagcucu agaagcugaa      900

auggauggag caaaagggaa auuauucagu ggacacuuaa aaugucgacu aaaaauggau      960

aaguugaggc uaaaaggugu aaguuauuca uuauguacag cagcauucac auuuacuaaa     1020

guaccagcag aaacauugca uggaacggua acaguggaag uccaauaugc aggaacggau     1080

ggaccaugca aaauaccagu acagauggca guagauaugc agacauuaac accaguagga     1140

agacuaauaa cagcgaaucc aguaauaaca gaaaguacag aaaauaguaa aaugauguug     1200

gaauuagauc cccccuuugg agauucauac auaguaauag gaguaggaga uaaaaaaaua     1260

acucaccauu ggcauaggag uggaucaaca aucggaaaag cauuugaagc aacuguaaga     1320

ggagcaaaaa ggauggcagu acuaggagau acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9327
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 3 opt

<400>  9327
gggagaaagc uuaccaugag ucaaaaaguc auauauuuag uaaugauauu auuaauugca       60

ccugcauaua guauaagaug uauaggagua aguaauagag acuuuguaga aggaaugagu      120

gguggaacau ggguagacgu agucuuagaa cauggaggau guguaacugu aauggcacaa      180

gacaaaccaa caguugauau agaauuaguu accacaacag uaaguaauau ggcagaagua      240

aggaguuauu guuaugaagc aaguaucagu gacauggcaa gugauagucg auguccaaca      300

caaggagaag cauaucuaga uaaacaauca gauacacaau auguauguaa aagaacauua      360

guagauagag gaugggguaa cgguugugga cuauuuggua aaggaagcuu aguaacuugu      420

gcaaaauuca cauguucaaa aaaaaugaca ggaaaaucaa uacaaccaga aaaucuggaa      480

uacagaauaa uguuaucagu acauggaagu caacauagug gaaugacagu aaacgauaua      540

ggauaugaaa cagaugaaaa uagagcaaaa guagaaguaa cuccaaauag cccaagggca      600

gaagcaacac uaggagguuu uggaucacug ggauuagauu gcgaaccaag aacuggacuc      660

gacuucagcg auuuauacua uuuaacuaug aauaauaaac auugguuagu ucauaaagaa      720

ugguuucacg acaucccauu accauggcac gcaggagcgg auacagguac accacacugg      780

aacaauaaag aggccuuagu agaauuuaaa gaugcacaug caaaaaggca aacuguagua      840

guauugggaa gucaagaggg agcaguacau acagcacuag caggugcauu agaagcagaa      900

auggacggag caaaagguaa acuauuuuca ggacaucuaa aaugcagauu aaaaauggac      960

aaauuacguu uaaaaggagu aaguuacuca cuuuguacag cggcuuuuac auucacaaaa     1020

guaccagcag aaacauuaca uggcacaguu acaguagaag uacaauaugc aggaacagac     1080

ggaccaugua aaauaccagu acaaauggca guagacaugc aaacacuaac cccgguagga     1140

agacuuauaa cagcaaaucc aguaauaaca gaaaguacug aaaauucaaa aaugaugcua     1200

gaauuagauc cuccauuugg agauucauac auaguaauag guguagggga uaaaaaaauu     1260

acacaccauu ggcauagauc cggaagcaca auaggaaaag cauuugaagc aacgguaaga     1320

ggagcuaaaa gaauggcugu acuaggagau acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9328
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 3 opt

<400>  9328
gggagaaagc uuaccauguc gcaaaaaguu auauaucuag uaaugauauu auuaauagca       60

ccagcuuaua guauaagaug uauaggagua ucaaacagag auuuuguaga aggaaugagc      120

ggagggacau ggguagacgu aguauuagaa cauggagguu guguaacagu aauggcacaa      180

gauaaaccaa cggucgauau agaauuggua acgaccacgg uaucaaauau ggcagaagua      240

agaaguuauu guuaugaagc auccauaagc gauauggcaa gcgauaguag auguccaaca      300

caaggagaag cauaucuuga uaaacaauca gauacccaau auguauguaa aagaacauua      360

guagauagag gauggggaaa ugguugugga uuguucggaa aaggauccuu aguaacaugc      420

gcaaaauuua cauguagcaa aaaaaugacu ggaaaaagua uacaaccuga aaaucuagaa      480

uauagaauaa uguuaucugu acauggaagu caacauucag gaaugauugu aaaugacgaa      540

aauagagcca aaguagaagu gacaccaaau agcccaagag cagaagcaac auuaggaggu      600

uuugguagcu uaggauuaga cugugaacca cgaacagggc uggacuuuuc agacuuauau      660

uaucuaacua ugaauaauaa acauuggcuc guacauaaag aaugguuuca cgauauacca      720

cuaccauggc augcaggagc agauacaggc acaccacauu ggaauaauaa agaagcauua      780

guagaauuca aagacgcaca cgcaaaaaga caaacaguag uaguacuagg aucacaagaa      840

ggagcaguac auacagcuuu agcaggagcu cuagaagcag aaauggaugg agcaaaaggu      900

agacuauuca gcggacauuu aaaauguaga uuaaaaaugg auaaacuaag guuaaaagga      960

guaaguuaua guuuauguac agcagcauuu acauuuacga aaguaccagc agaaacauug     1020

cacggaacag ugacaguuga aguacaauau gcgggaacag acggaccaug caaaguacca     1080

gcacagaugg ccguagauau gcaaacauua acaccaguag gucgacuaau aacugcaaau     1140

ccaguuauua cagaaucuac agaaaauagc aaaaugaugc uagaguuaga uccuccauuc     1200

ggagacaguu auauaguaau aggaguugga gauaaaaaaa uaacacauca uuggcauaga     1260

aguggaagua caauaggaaa agcauuugaa gcaacaguaa gaggagcuaa aagaauggcu     1320

guuuuaggag auacagcaug ggacuuugga aguugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  9329
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 3 opt

<400>  9329
gggagaaagc uuaccauguc acaaaaagua auauacuuag uuaugauacu auuaauagca       60

ccugcuuaca gcauacgaug cauuggugua agcaaucgug acuuuguaga gggaaugagc      120

gguggaacau ggguagacgu aguauuagaa cacggaggau guguaacagu aauggcgcaa      180

gauaaaccaa ccguagauau agaauuaguu acaacaacag uaucaaauau ggcagaagua      240

agaucauauu guuaugaagc aagcauauca gauauggcaa gugacagucg augcccaaca      300

caaggagaag cauauuuaga uaaacaaagc gauacacaau acguauguaa aagaacauua      360

guagauaggg gauggggcaa cgguugcgga uuauucggca aaggaagccu aguuacaugu      420

gccaaauuua cauguaguaa aaaaaugacu ggaaaaucaa uacaaccuga aaauuuagaa      480

uauagaauua ugcuaagugu acacggaagc caacacucag gaaugauugg auacgaaaca      540

gaugaaaaca gggcuaaagu agaagucaca ccaaauucac caagagcaga agcaacacuu      600

ggaggcuucg guucgcuagg acucgauugu gaaccaagaa caggacuaga cuuuucugac      660

uuauauuauc uaacaaugaa uaauaaacau uggcuaguac auaaagaaug guuccacgau      720

auaccauugc cauggcacgc aggugcagau acaggaacac cacauuggaa uaacaaagaa      780

gcauuaguag aauuuaaaga cgcccaugcu aaaagacaaa caguaguagu acuaggaagu      840

caagagggug caguccauac agcauuagca ggagcacuag aagcagaaau ggauggggcu      900

aaagguaaau uauuuagugg acauuuaaaa uguagauuaa aaauggacaa auuaagauua      960

aaaggaguaa guuacagcuu auguacagca gccuucacau uuacaaaagu accagcagaa     1020

acauuacaug gaacaguuac aguagaagua caauaugcag gaacagaugg accauguaaa     1080

auaccagucc aaauggcagu agauaugcaa acauuaacac caguaggaag auuaauaaca     1140

gcaaauccug uaauaacaga aaguacagaa aauagcaaaa ugauguuaga acuagaucca     1200

ccauuuggug acaguuauau agugauagga guuggagaca aaaaaauaac acaucauugg     1260

caucgaucag gaaguacaau uggaaaagca uucgaagcaa cuguaagagg agcaaaaaga     1320

auggcugucu uaggggacac agcaugggau uucggcaguu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  9330
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 3 opt

<400>  9330
gggagaaagc uuaccauguc ucaaaaagua auauauuuag uuaugauacu auugauagcu       60

cccgcauaua gucuaagaug uauaggagua agcaauagag auuuuguaga aggaaugagc      120

gguggaacau ggguagaugu aguauuagaa cauggaggau guguaacagu uauggcacaa      180

gauaaaccaa caguagauau agaauuagua acaacaacag uaucaaauau ggcagaagua      240

agaucauauu guuaugaagc aaguauaagc gauauggcuu cagauaguag augcccaacc      300

caaggagaag cauaccuaga uaaacaaagu gauacacaau acguauguaa aagaacguua      360

guagauagag gauggggaaa uggaugcgga uuauuuggaa aaggaagucu aguaacaugu      420

gcuaaauuug cauguucaaa gaaaaugaca ggaaaaucaa uucaaccaga aaauuuagaa      480

uauagaauaa uguuaagcgu acaugguuca caacauagug gaaugaucgu aaaugauaca      540

ggacaugaaa cagaugaaaa ucgagcuaaa guagaaauaa caccuaacag uccaagagca      600

gaagcaacau uaggaggauu uggaaguuua ggauuggauu gcgaaccaag aacagguuua      660

gauuucucag auuuauauua ucuaacaaug aauaacaaac auugguuggu acauaaagaa      720

ugguuucaug auauaccucu accuuggcau gcaggagcag acacgggaac accacacugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcucaug caaaaagaca aacuguagua      840

guauuaggaa gucaagaagg agcaguucau acagccuuag caggagcauu agaagcagaa      900

auggauggag cuaaaggaag auuaucaagu ggacauuuga aaugcagauu gaaaauggau      960

aaauuaagau uaaaaggagu aucauauagu cuauguacag cagcauuuac auuuacaaaa     1020

auaccagcgg aaacacuaca uggcacaguu acugucgaag uacaauaugc aggaacagac     1080

gguccaugua aaguaccagc acaaauggca gucgauaugc aaacauuaac accaguggga     1140

agauuaauaa cagcaaaucc aguaauaacu gaaucaacug aaaauucaaa aaugaugcua     1200

gaauuagauc cacccuucgg ugauucguac auaguaauag gaguugggga aaagaaaaua     1260

acacaucauu ggcauagauc aggaaguaca auuggaaaag cauucgaagc uacuguuaga     1320

ggcgcuaaaa gaauggcagu auugggagau acagcauggg auuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9331
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 3 opt

<400>  9331
gggagaaagc uuaccaugag caaaaaagua auauaccuag uaaugauauu acuaauagca       60

ccagcauaca guauacgaug uauaggagua aguaacagag auuuuguuga aggaauguca      120

ggaggaacau ggguugaugu aguauuagaa cacggcggau guguaacagu aauggcacaa      180

gauaaaccaa caguugauau agaauuagua accacaacag uuagcaauau ggcugaagua      240

agaucauauu guuaugaagc aucaauauca gauauggcaa gugauucaag auguccaaca      300

caaggugaag cauaucuaga caaacaauca gauacacaau acguauguaa aagaacacua      360

gucgauagag gauggggaaa uggaugcggu uuauucggaa aagggucacu aguuacaugu      420

gccaaauuug caugcagcaa aaaaaugacu ggaaaaucaa uucaaccaga aaaucuugaa      480

uauagaauaa uguuaucagu acaugguuca caacauagcg gaaugauugu uaacgacacc      540

ggacaugaaa cagaugaaaa ucgagcaaaa guagaaauaa cuccaaacuc accaagagcu      600

gaagcaacau ugggaggauu cggaucauua ggacuagacu gugaaccaag aaccggauua      660

gauuuuucag acuuauauua uuuaacaaug aauaacaaac auugguuagu acacaaagaa      720

ugguuucacg acauaccacu accauggcac gcaggagcug auacagguac accacauugg      780

aauaacaaag aagcucucgu agaauuuaaa gaugcacaug caaaacgaca aacuguaguc      840

guauuaggau cucaagaagg agcuguacau acagcacuag caggagcauu agaagcagaa      900

auggauggag cuaaaggaag auuaagcuca ggccaucuca aaugccgauu aaaaauggau      960

aaauuaagau uaaaaggagu aucauauuca uuauguacag cagcauucac auuuacaaaa     1020

auuccagcug aaacauuaca ugguacugua acaguagaag uacaauaugc cgguacagau     1080

gguccaugua agguaccugc acaaauggca guagauaugc aaacauuaac accaguagga     1140

agacuaauaa cagcaaaucc uguaauaaca gaaaguacag aaaauucaaa aaugaugcua     1200

gaauuagacc caccguucgg cgauaguuau auuguaauag gaguaggaga aaaaaaaaua     1260

acucaccauu ggcauagauc aggcucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcuaaaa gaauggcugu ucuaggagac acagcauggg auuuugguuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9332
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 3 opt

<400>  9332
gggagaaagc uuaccaugag ccaaaaagua aucuauuuag ucaugauauu auugauagcc       60

ccagcauacu cuauaagaug uauaggagua aguaauagag acuucgucga aggaaugagu      120

ggaggaacau ggguagaugu aguucuagaa cauggaggau guguaacagu aauggcucaa      180

gacaaaccca caguagauau agaauuagua acaacaacag uaagcaauau ggcagaagua      240

cgaaguuauu gcuaugaagc aucaauauca gauauggcau cagauucaag auguccaacc      300

caaggagaag cuuaucucga uaaacaauca gauacacaau auguauguaa aagaacucua      360

guagauaggg gauggggaaa ugguugcggu cuauuuggaa aaggaaguuu agucacaugu      420

gcaaaauuug caugcaguaa aaaaaugaca ggaaaaucua uacaaccaga gaacuuagaa      480

uauagaauaa uguugagugu acacgguagu caacauagug guaugauagu caaugauaca      540

ggacaugaaa cagaugaaaa uagagcaaag guagaaauaa cgccaaauuc accaagagca      600

gaagcaacau uaggaggauu uggaagcuua ggacuagacu gugaaccacg aacgggauua      660

gauuuuagug accuauauua uuuaacaaug aauaacaaac auuggcuggu ucauaaagaa      720

ugguuucacg auauaccacu accauggcau gcuggagcag auacagguac accacacugg      780

aacaauaaag aagcauuagu agaauuuaaa gacgcucaug caaaaagaca aacgguagua      840

gucuuaggau cacaagaagg agcaguacac acagcauuag cgggagcacu agaagcagaa      900

auggauggug caaaaggaag auuaaguagu ggacaucuaa aauguagauu aaaaauggau      960

aaauuaaggu uaaaaggagu aaguuauuca uuauguacgg cggcauuuac auuuacaaaa     1020

auaccugcag aaacguuaca uggaacaguu acagucgaag uucaauaugc uggaacagau     1080

ggacccugua aaguuccagc acaaauggcg guagauaugc aaacauuaac accaguugga     1140

agauuaauca cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugaugcuu     1200

gaauuagauc caccauucgg agauagcuau auaguaauug gaguagguga aaaaaaaaua     1260

acgcaucauu gguauagauc aggaucaaca auaggaaaag cauuugaagc aacuguaagg     1320

ggagcaaaac guauggcagu auugggagau acagccuggg acuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9333
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 3 opt

<400>  9333
gggagaaagc uuaccauguc acaaaaagua auauaucuag uaaugauauu acuuauagcu       60

ccugcauaua guauacgaug cauaggggug ucaaauagag auuuuguaga aggaaugagu      120

gggggaacau ggguagaugu aguacuagaa cacggagguu guguaacagu cauggcacaa      180

gauaaaccga cuguggauau agaacuugua acaacuacag uaucaaauau ggcagaagua      240

agaaguuauu gcuacgaagc aaguauaagc gacauggcaa gcgauuccag guguccaaca      300

caaggagaag cauaccuaga uaaacaaagc gauacucaau auguauguaa aagaacgcuc      360

guagauagag gcuggggaaa uggaugugga uuauuuggua aagggaguuu aguuacaugu      420

gcuaaauuug caugcaguaa gaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa      480

uaucgaauaa uguugucagu ucauggaagu caacauucag gaaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa uagagcgaaa guugaaauaa caccaaauuc accaagagca      600

gaagcaacac uagggggauu ugggucacua ggacuagauu gugaaccaag aacaggauua      660

gauuuuucag aucucuacua ccuaacaaug aacaauaaac acugguuagu acauaaagaa      720

ugguuucacg acaucccauu accauggcau gcaggagcag auacaggaac gccacauugg      780

aauaacaaag aagcauuagu agaauuuaaa gaugcccaug cuaaaagaca aacaguugua      840

guacuugguu cacaagaagg ugcaguacac acagcauuag caggagcauu agaagcagaa      900

auggauggag caaaaggaaa acuaagcucu ggacaucuaa aaugcagacu aaaaauggau      960

aaauuaagau uaaaaggagu aagcuauagu cuaugcacag cagcauucac uuucacaaaa     1020

auaccagcag aaacauuaca cggaacagua acgguagaag uacaauaugc aggaacugac     1080

ggaccaugua aagugccagc acaaauggca guagauaugc agacauuaac accaguaggu     1140

agauuaauaa cagcaaaccc ggugauuacu gaaagcacug aaaauaguaa aaugauguua     1200

gaacuagauc caccuuuugg agauaguuau auaguuauag gaguaggaga aaagaaaaua     1260

acacaccacu ggcacagauc aggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcugu acuaggagau acagcauggg acuuugguag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9334
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 3 opt

<400>  9334
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauacu ccucaucgca       60

ccagcauacu caauaagaug uauaggagua ucaaacagag acuuuguaga aggaaugucc      120

ggaggaacuu ggguugaugu uguauuagaa cauggaggau guguaaccgu aauggcacaa      180

gacaaaccaa caguagauau ugaacuagua acaacaacag uaucaaauau ggcagaaguu      240

agaaguuauu guuacgaagc aucaauauca gauauggcau cagauucaag gugcccaaca      300

caaggagaag cauauuuaga caaacaauca gacacacaau auguaugcaa aagaacacug      360

gucgauagag gauggggaaa cggaugugga uuguucggaa aaggaucauu aguuaccugu      420

gcaaaauucg caugcagcaa aaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa      480

uacagaauaa uguuaagugu ucacggauca caacauagug gaaugauugu uaacgacaca      540

ggacacgaaa cagaugaaaa uagagcaaaa guagaaauaa cgccaaauuc gccaagagca      600

gaagcaacau uagguggauu uggaagcuua gguuuagauu gugaaccaag aacaggacuu      660

gauuuuagcg auuuauacua ccuaacaaug aauaauaaac auugguuagu ucacaaagaa      720

ugguuucaug auauaccauu accauggcac gcaggagcag auacaggaac accacacugg      780

aauaacaaag aagcacuugu cgaauuuaaa gaugcgcacg caaaaagaca aacaguaguu      840

guucuaggau cacaagaagg agcaguacau acagcucuag cuggagcacu agaagcagaa      900

auggacggag caaaaggcag auuauccagu ggacauuuaa aauguagacu aaaaauggac      960

aaauuaagac uuaaaggagu uaguuauuca cuauguacag ccgcauucac auucacaaaa     1020

auuccagcag aaacacuaca uggaacagua acagucgaag uacaauaugc aggaacagau     1080

ggaccaugua aaguaccggc acaaauggcu guagacacuc agacauuaac accaguagga     1140

agguuaauua cagcaaaucc aguaauaacu gaaucaacag aaaauagcaa aaugauguua     1200

gaauuagauc caccauuugg ugauaguuau auuguuauag gaguaggaga aaaaaaaaua     1260

acacaucauu ggcaucgauc aggaagcaca auaggaaaag cauuugaagc cacuguaaga     1320

ggagcaaaaa gaauggcagu acucggugau acagcguggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9335
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 3 opt

<400>  9335
gggagaaagc uuaccauguc acaaaaagua auauaccuag uaaugauauu auuaauagcu       60

ccagcauaua guauaagaug cauaggagua aguaaucgag auuuuguaga gggaaugagc      120

ggggguacau ggguagacgu aguauuggaa cacggaggau guguaacagu aauggcacaa      180

gacaaaccaa caguagacau ugaacugguu acgaccacug uaucaaauau ggcagaaaua      240

agaucauacu gcuaugaagc gaguauaagu gauauggcau cagauucuag auguccaaca      300

caaggagaag cauaucuaga caaacaaagu gauacacagu auguauguaa aagaacgcua      360

guagauagag gauggggaaa uggauguggu cuauuuggga aagguagcuu aguaacaugu      420

gcaaaauuug caugcaguaa aaaaaugaca ggaaaaucaa uucaaccaga aaauuuagaa      480

uauagaauaa uguuaucugu gcauggauca caacauucag gaaugauagu gaacgauaca      540

ggacacgaaa cugaugaaaa uagagcaaaa gucgaaauaa ccccaaauuc acccagagcc      600

gaagcaacau uaggaggauu uggaucacua ggccuugauu gugaaccaag aacaggauua      660

gacuuuucag acuuguauua ucuaacaaug aauaacaaac auugguuagu acauaaagaa      720

ugguuucaug auauaccauu accuuggcau gcaggagcag auacggguac accacauugg      780

aauaauaaag aagcccucgu agaauuuaaa gaugcacaug cuaaaaggca aacuguugua      840

guacuaggua gucaagaagg agcuguacau acagcauuag caggagcauu agaagcagaa      900

auggacgggg caaaagguag gcucaguuca ggacaccuaa aaugcagauu aaaaauggau      960

aaauuaagac uaaaaggcgu aaguuauagu uuauguacag cagcauuuac auucacaaaa     1020

auaccagcag aaacguuaca uggaacagua acaguagaag uacaauaugc aggaacagau     1080

ggaccuugca aaguuccagc acaaauggca guagauaugc aaacacuaac accaguagga     1140

agacuaauaa ccgcaaaucc aguaauaaca gagagcacag aaaauagcaa aaugaugcua     1200

gaauuagacc caccauucgg agauuccuau auaguaauag gaguagguga aaaaaaaauu     1260

acacaucacu ggcaccgauc aggaucaaca auagguaagg cuuucgaagc aacaguaaga     1320

ggagcuaaac guauggcagu auuaggagau acugcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9336
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 3 opt

<400>  9336
gggagaaagc uuaccauguc acaaaaagua auauacuuag uuaugauuuu guuaauagca       60

ccagcauauu caauaagaug cauuggagua ucaaauagag auuucguaga aggaaugagc      120

ggaggcacau ggguagaugu aguauuagaa caugguggau guguuacugu aauggcacaa      180

gauaaaccaa cuguagacau agaacuagua acaacaacag uaucaaauau ggcagaagua      240

agaaguuauu guuaugaagc aagcauaucc gauauggccu cagacaguag auguccaaca      300

caaggagaag cuuacuuaga uaaacaauca gauacacaau auguauguaa aagaacacua      360

guagacagag gauggggaaa uggaugcggu uuauucggaa aaggaagucu aguaacaugc      420

gcaaaauuug cauguaguaa aaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa      480

uacagaauaa ugcuaagugu ccauggaagu caacauagug gaaugauugu aaaugacaca      540

ggacaugaaa cagaugaaaa uagagcgaaa guagaaauaa caccaaacuc accaagagca      600

gaagcaacau uaggaggauu uggaucauua ggucuugauu gugaaccaag aacuggacuu      660

gauuuuucag auuuauauua ccuaacaaug aauaauaaac acuggcuggu acauaaagaa      720

ugguuccaug acauaccucu acccuggcac gcaggagcag auacagguac cccacauugg      780

aauaauaaag aagcauuagu agaguucaaa gaugcacaug caaaaagaca aacaguaguu      840

guauuaggau cacaagaagg agcaguacau acagcacuag caggagcauu agaagcagaa      900

auggacggcg caaaaggcag acuuagcagu ggacaccuaa aaugccgcuu aaaaauggau      960

aaacuaagau uaaaaggagu aaguuauuca uugugcacag cagcauucac auucacaaaa     1020

auacccgcag aaacacuaca cgguacaguu acaguugaag uacaauaugc aggaacagau     1080

ggaccaugua aaguuccagc acaaauggca guagauaugc aaacauuaac accaguaggu     1140

cgauuaauaa cagcaaaccc aguaauuaca gaaucaacag aaaauaguaa aaugauguua     1200

gaacucgacc ccccauucgg agacucauac auaguuauag gaguagguga aaaaaaaauu     1260

acacaucacu ggcacagauc aggaucaaca auaggaaaag cuuuugaagc aacaguuaga     1320

ggagcacgaa gaauggcugu auuaggagau accgcguggg acuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9337
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 3 opt

<400>  9337
gggagaaagc uuaccauguc ucaaaaaguu auauauuuag uaaugauuuu auuaauagca       60

ccagcauauu caauuagaug uauaggagua ucaaauagag auuuuguaga gggaaugucu      120

ggaggaacau ggguagaugu aguucuagaa cauggaggau guguaaccgu aauggcgcaa      180

gauaaaccaa cugucgauau agaauuagua acaacaaccg uuucaaauau ggcagaagua      240

agaucauauu guuaugaagc aucaauaagu gauaugguaa gcgacucaag auguccaaca      300

caaggagaag cauaucugga uaaacaaucg gacacgcaau auguauguaa aaggacucua      360

guugauagag gauggggaaa uggaugcggu uuauuuggua aaggaaguuu aguaacaugc      420

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaucca uacaaccuga aaauuuagaa      480

uacagaauaa uguuaucagu acauggaagu caacauucag gaaugauagu aaacgacaca      540

ggacaugaaa cagaugaaaa cagagcuaaa guggaaauaa caccgaauag cccaagagca      600

gaggcaacau uaggaggauu uggaucacug ggauuagauu gugaaccaag aacaggcuua      660

gauuuuagcg acuuauacua uuuaacaaug aauaauaaac auuggcuagu gcauaaagaa      720

ugguuccacg auauaccauu accguggcau gcaggagcag auacaggaac accacauugg      780

aauaacaaag aagcuuuagu agaauuuaaa gacgcccaug caaaaagaca aacgguagua      840

guauugggau cacaagaagg ugcaguacau acagcacuag caggagcauu agaagcagaa      900

auggauggcg caaaaggaag acuaucguca ggacacuuaa aauguagauu aaagauggac      960

aaacuuagau uaaaaggcgu aaguuauuca uugugcacag cagcauuuac auuuacaaaa     1020

auaccugcag aaacauuaca uggaacagua acuguagaag uacaauaugc aggaacagac     1080

gguccaugca aaguaccagc acaaauggca guagauaugc aaacacuaac accaguagga     1140

agacuaauaa ccgcaaaucc agucauaaca gaaucaacag aaaauagcaa aaugaugcua     1200

gaauuagauc cuccauucgg agauagcuau auagucauag ggguugggga aaaaaaaauu     1260

acucaccacu ggcauagaag uggaagcacc auaggaaaag cauuugaagc aacaguaaga     1320

ggagccaaaa gaauggcagu guuaggagau acggcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9338
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 3 opt

<400>  9338
gggagaaagc uuaccaugag ucaaaaagua auauaccuag uaaugauacu auuaauagca       60

ccugcauaua guauaagaug uauaggagua ucaaauagag auuucguaga aggaauguca      120

ggaggcacau ggguagacgu aguuuuagaa cauggaggau guguaacugu aauggcacaa      180

gauaaaccua cuguagauau agaauuagua acaacaacag uaucaaacau ggcagaagua      240

agaaguuauu guuaugaagc aagcauaucu gauauggcau cagacucaag augcccuaca      300

caaggagaag cguacuuaga caaacaaagc gauacacaau auguauguaa aagaacacuc      360

guagauagag gaugggggaa cggauguggu uuauucggga aaggaucccu aguaacaugu      420

gcaaaauuug cauguaguaa aaaaaugaca gggaaaucaa uacaaccuga aaaccuagaa      480

uauagaauaa uguuaagcgu acauggauca caacauucag gaaugauugu aaacgacaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauua caccaaauuc accaagggcu      600

gaagcaacau uaggagguuu uggaaguuua ggauuagauu gugaaccaag aacaggacua      660

gauuucagug auuuguacua cuuaacaaug aauaauaaac auuggcuugu ucauaaagaa      720

ugguuccaug auauaccacu accauggcac gcaggagcag auacuggaac accucacugg      780

aacaauaaag aagcacuagu ugaauucaaa gacgcccaug caaaacgaca aacugucgua      840

guauuaggau cacaagaagg agcaguacau acagcauuag caggagcauu agaagccgaa      900

auggauggag cuaaaggaag auuaucauca ggacaucuaa aguguaggcu aaaaauggac      960

aaacuaagau uaaaaggagu gaguuacagu uuauguacag ccgcauuuac auuuacaaaa     1020

aucccagcag aaacacugca uggaacugua acaguugaag uacaauaugc aggaacagau     1080

gggccaugca aaguaccagc acaaauggca guagauaugc aaacacuaac accaguagga     1140

agguuaauaa cagcuaaucc aguaauaaca gaaaguauag aaaauaguaa aaugauguua     1200

gaacuagacc caccuuucgg ugauucuuau auaguaauag gaguagguga aaaaaaaaua     1260

acacaucauu ggcauagauc uggaaguacc auaggaaaag cauuugaagc uacaguaaga     1320

ggagcuaaaa gaauggcagu auuaggagau acagcauggg auuucgguag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9339
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 3 opt

<400>  9339
gggagaaagc uuaccauguc acaaaaagua auauaccuag uaaugauauu acuaauagca       60

ccagcuuacu caauaagaug uauaggggua ucaaauagag auuuugucga aggaaugucu      120

ggaggaacau ggguagaugu cguacuugaa cacggaggau guguaacagu aauggcacaa      180

gauaaaccaa caguagauau agaauuaguu acaacaacag uaucaaacau ggcugaagua      240

agaucauacu guuacgaagc cucaauuuca gauauggcaa gugauucuag guguccaacg      300

caaggagaag ccuauuuaga uaaacaaucc gauacacaau auguuuguaa aagaacuuua      360

guagauagag gaugggguaa cggaugugga uuauucggaa aagggucucu aguaacaugu      420

gcaaaauuug cauguaguaa aaaaaugaca ggaaaaucaa uacaaccuga aaaucuagaa      480

uacagaauaa uguuaagugu acauggaagc caacauucag gaaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa ccgugccaaa guagaaauaa caccaaacuc accacgagca      600

gaagcaacuu uaggaggauu ugggucacua ggacuagacu gugaaccaag aacuggacuu      660

gacuuuucug aucuauauua uuuaaccaug aauaacaaac acuggcuugu acacaaagaa      720

ugguuccaug auaucccauu accauggcac gcaggagcag auacaggaac accccauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaacgaca aacagucguu      840

guauuaggau cacaagaagg agcaguacau acagcacuag caggagcacu agaagcagaa      900

auggauggag caaaaggaag auuaucaagc ggacaccuaa aauguagacu aaaaauggau      960

aaauuaagau uaaaaggagu aaguuauagu uuauguacag cagcauucac auucacaaaa     1020

auaccagcag aaacacuaca uggaacaguc acaguagaag uacaauaugc uggaacagau     1080

ggaccuugua aaguaccagc acaaauggca guagauaugc aaacauuaac uccaguagga     1140

agauuaauua cagcaaaucc uguaauaaca gaaucaaccg aaaacucaaa aaugauguua     1200

gaauuagacc caccauuugg agacucauau auagucauag gaauaggcga aaaaaaaaua     1260

acacaucacu ggcacagaag uggcucaaca auaggaaaag cauuugaagc gacaguaagg     1320

ggagcaaaaa gaauggccgu auuaggagac accgcauggg acuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9340
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 3 opt

<400>  9340
gggagaaagc uuaccauguc gcaaaaagua auauaucugg uuaugauuuu guuaauagca       60

uuagcauacu caauaagaug cauaggagua aguaauaggg auuuuguaga aggaaugagu      120

ggagguacau ggguagaugu aguauuagaa caugguggau gcguaacagu aauggcacaa      180

gauaaaccua caguagacau agaauuagua accaccacag uuucaaacau ggcagaaguc      240

agaucauacu guuacgaagc aagcauaucc gauauggcau cagacucuag auguccaacc      300

caaggugaag cauauuuaga caaacaauca gacacacaau auguauguaa acgaacuuua      360

guagauagag gauggggaaa ugguugcgga uuauuuggaa aaggaucauu aguaacaugu      420

gcaaaauucg caugcaguaa aaaaaugacu ggaaaaucaa uacaacccga aaauuuagaa      480

uauagaauaa uguuaagugu ucauggcagu caacauagug guaugauagu aaaugacaca      540

ggacaugaaa cagaugaaaa cagagcaaaa guugaaauaa caccaaacuc uccaagagca      600

gaagcaacau uaggagguuu ugguaguuua ggacuagauu gugaaccaag aacuggauua      660

gauuuuucag aucuauauua uuugacaaug aauaauaaac auugguuagu ucacaaagaa      720

ugguuccaug auauaccacu accauggcau gcaggagcag auacaggaac accacauugg      780

aauaauaaag aagcauuagu ggaauuuaaa gaugcacaug caaaacgaca aacaguaguu      840

guacuaggau cacaagaagg agcaguacac acggcauuag caggagcauu agaagcagaa      900

auggaugggg caaaaggaag guuaagcucc gggcauuuaa aaugccgauu aaaaauggac      960

aaauuaagau uaaaaggugu gucuuauagu cuauguacag cagcauuuac auuuacaaaa     1020

auaccggcag aaacacuuca ugguacagua acaguagaag uacaauaugc aggaacagau     1080

ggcccaugua aagugccagc acaaauggca guagacaugc aaacacuaac accagucgga     1140

agauuaauaa cugcaaaucc aguaauaaca gaaucaacag aaaacaguaa aaugauguua     1200

gaauuagacc cuccguucgg agacuccuau auaguaauag gaguagguga aaaaaaaaua     1260

acacaucauu ggcauagauc aggaucaaca auaggaaaag ccuuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu acuaggagac acagcauggg acuuuggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9341
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 3 opt

<400>  9341
gggagaaagc uuaccauguc acaaaaagua auuuacuuag uuaugauacu acuuauagca       60

ccagcauauu caauaagaug uauaggagua ucaaauaggg acuuuguaga aggaaugagu      120

ggugguacuu ggguagacgu aguauuagaa cacggaggau guguaacugu aauggcgcaa      180

gauaaaccaa caguagacau agaauuaguu acaaccaccg uaaguaauau ggcagaagua      240

agaaguuauu gcuacgaagc aaguauauca gacauggcaa gugauucaag auguccaacu      300

caaggugaag cauauuuaga uaaacaauca gauacacaau auguauguaa aagaacucua      360

guagacagag gaugggguaa uggaugcggc uuauucggaa aaggauccuu aguuacaugu      420

gcuaaauuug cuuguaguaa gaaaaugaca ggaaaaagua uucaaccaga aaacuuagaa      480

uacagaauaa uguugagcgu acaugguagu cagcauucag gaaugauagu caaugauaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccaaauag uccaagagca      600

gaagcgacau uaggaggauu uggaagccua ggacuagacu gugaaccaag aaccgguuua      660

gauuucuccg auuuauacua uuuaacaaug aacaacaaac acuggcuagu ccauaaagaa      720

ugguuucaug auauuccacu accauggcau gcaggagcag acacggguac accacacugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacaug caaagagaca gacaguaguu      840

guauuaggaa cacaagaagg ugcaguacac acagcacuag cuggugcacu agaagcagaa      900

auggauggag caaaaggccg acuaaguagu ggacaccuua aauguagauu aaaaauggau      960

aaauuaagau uaaaaggagu uucuuauagu uuauguacag cagcauucac auuuacaaaa     1020

auaccagcug aaacauuaca cggaacagua acaguugaag uucaauaugc aggaacagau     1080

ggaccaugua aaguaccugc gcaaauggcu guagauaugc aaacguuaac accaguagga     1140

agauuaauaa cugcaaaccc aguuauaaca gaaucuacag aaaauaguaa aaugauguua     1200

gaauuagacc cuccauuugg cgauaguuau auaguaauag gaguaggaga aaagaaaaua     1260

acacaccacu ggcauagauc aggaaguaca auaggaaaag cauuugaagc uacaguacga     1320

ggcgcuaaaa ggauggcugu auuaggugau acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9342
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 3 opt

<400>  9342
gggagaaagc uuaccaugag ccaaaaaguu auauauuuag uuaugauacu auuaauagca       60

ccagcuuaca guauaagaug uauaggagua aguaauagag auuuuguaga ggguauguca      120

ggaggaacau ggguagacgu aguauuagaa cauggaggau guguaacagu aauggcacaa      180

gauaaaccaa caguagauau agaacuagua acgacaacag uaucaaauau ggcugaaguu      240

aggucauauu guuaugaagc aaguauuagu gacauggcau cagauagcag auguccaaca      300

caaggagaag cauaucuaga caaacaaagu gauacacaau auguaugcaa aagaaccuua      360

guagauagag gauggggaaa cggcuguggu cuauuuggaa aaggaucauu aguaacaugc      420

gcaaaauuug caugcaguaa aaaaaugaca ggaaaaucaa uacaacccga aaaucuagaa      480

uauagaauaa uguuaagugu acauggauca caacacucgg gcaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa uagagcgaaa guagaaauaa caccaaauag uccuagagcc      600

gaagcaacgu uaggaggauu uggcucacuu ggacuggauu gugaaccaag aaccggacua      660

gauuuuucag auuuauauua uuuaacaaug aacaauaaac auugguuagu acacaaagaa      720

ugguuucaug auauccccuu accauggcac gcaggugcag auacaggaac accacauugg      780

aauaauaaag aggcauuagu agaauuuaaa gacgcacaug caaaaagaca aacaguagua      840

guauuaggaa gucaagaagg agcaguacau acagcauuag cuggagcccu agaagcggaa      900

auggacggag caaaaggaag guuaucaagu ggacauuuaa aauguagacu aaaaauggau      960

aaauuaagac uaaaaggagu aucauacagc uuguguacag cagcauuuac auuuacaaaa     1020

auaccagcag aaacauuaca cgguacaguu acaguagaag uacaauaugc aggaacagac     1080

ggaccuugca aaguauuagc acaaauggcc guagauaugc aaacacuaac accaguagga     1140

agauuaauaa cagcaaaucc aguaauaaca gaaagcacag aaaacaguaa aaugauguua     1200

gaacucgauc cacccuucgg agauuccuac auagucauag gugugggaga aaaaaaaauu     1260

acacaucauu ggcauagaag cggaagcaca auaggcaaag cauuugaagc aacagucaga     1320

ggagcaaagc gaauggcagu ucuaggagac acagccuggg auuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9343
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 3 opt

<400>  9343
gggagaaagc uuaccaugag ccaaaaagua auauauuuag uaaugauauu acuaauagca       60

ccagcauaua guauacgcug uauuggagua ucgaaucgag acuuuguaga aggaaugagc      120

ggaggaacau ggguagaugu aguacuagaa cacggaggau guguuaccgu uauggcacag      180

gauaaaccaa caguagauau agaauuagua acaacaacag uuaguaauau ggcagaagua      240

agaagcuauu guuacgaagc aucaauaagc gacauggcaa gcgauaguag augcccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacucaau auguaugcaa acgaacauua      360

guagacagag gauggggaaa uggaugugga uuauuuggaa aaggaagucu aguuacaugc      420

gcaaaauuug cuuguagcaa gaaaaugaca ggaaaaucaa uacaaccaga aaacuuggaa      480

uauagaauaa uguuaucugu acauggauca caacauucag gaaugauagu aaacgauacu      540

ggacacgaaa cagaugaaaa uagagcaaaa guugaaauua cacccaauag cccaagagca      600

gaagcaacau uagggggauu cggaucauua ggauuagacu gugaaccaag aacaggauua      660

gacuucucag auuuguauua uuuaacaaug aauaauaaac acuggcuagu acacaaagaa      720

ugguuucaug auauaccucu cccauggcac gcaggagcug auacaggaac accacacugg      780

aauaacaaag aagcauuagu agaauuuaaa gaagcacaug caaaaagaca aacuguugua      840

guacuaggaa gucaagaagg agcaguacac acugcauuag caggagcuuu agaagcggaa      900

auggauggag caaaaggaag auuaucaagu ggacaucuaa aauguagacu aaaaauggau      960

aaacuaagau uaaaaggcgu aaguuauagu cuauguacgg cugcguucac auucacaaaa     1020

auaccagcag aaacauuaca cggaacagua acaguagaag uacaauaugc aggcacagau     1080

ggacccugca aaguaccagc acaaauggca guagauaugc aaacauuaac accaguagga     1140

agacuaauaa cagcaaaucc uguaauaaca gaaaguaccg aaaauaguaa aaugauguua     1200

gaacuagauc caccauuugg agauaguuau auaguaaucg gaguuggaga aaagaaaaua     1260

acucaucacu ggcacagaag cgguaguacc auagguaaag cauuugaagc uacagugcga     1320

ggagcaaaaa gaauggcagu uuuaggagau acagcauggg auuuuggaag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9344
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 3 opt

<400>  9344
gggagaaagc uuaccauguc acaaaaagua auauacuuag uuaugauauu acucaucgcu       60

ccggcauaua guauaagaug uauuggagua ucaaauagag auuuuguaga aggaauguca      120

ggaggaacau ggguagaugu gguauuagaa cauggcggau gcguaacagu aauggcacaa      180

gauaaaccaa caguagacau agaauuaguc accacaacag ucagcaauau ggcagaagua      240

agaagcuauu gcuaugaagc aucaauaagu gauauggcaa gugauagcag auguccaaca      300

caaggagaag cuuaucuaga uaaacaaagu gauacacaau acguauguaa aagaacauua      360

guugacagag gauggggaaa cggcugugga cuauuuggaa aaggaaguuu aguaacaugc      420

gcaaaauucg cauguagcaa aaaaaugaca ggaaaaagua uucaaccaga aaaccuagaa      480

uauagaauaa uguuaagcgu acauggaagu caacauucag gaaugauagu uaacgauaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa ccccaaauag uccuagagca      600

gaagcagcau uaggaggauu uggaucacua ggauuagauu gcgagccaag aacaggguua      660

gauuuuucag auuuauauua ucuaacaaug aauaacaaac auugguuagu acacaaagaa      720

ugguuccacg auauaccauu accauggcac gcaggcgcgg acacaggaac accccauugg      780

aacaauaaag aagcauuagu agaauuuaaa gaugcgcaug caaaacgaca aacuguagua      840

guacuuggaa gucaagaagg agcugugcau acagcucugg caggagcuuu agaagcagaa      900

auggauggag caaaaggaag auuaucaagu ggucacuuaa aauguagauu aaaaauggau      960

aaacuaaggu uaaaaggugu gagcuacagu uuaugcacag cugcauuuac auuuaccaaa     1020

auaccugcag aaacacuaca cggcacaguu acuguagaag uccaauaugc aggaacagac     1080

ggaccaugua aagugccagc acaaauggca guagacaugc aaacauuaac accagugggg     1140

agacuaauaa cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugauguua     1200

gaauuagauc caccauucgg agauucauau auagucauag guguggguga aaaaaaaauu     1260

acacaucauu ggcauagauc aggaucaacg aucgguaaag cauuugaagc aacaguacgu     1320

ggggcgaaac gaauggcagu auuaggagau acggcauggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9345
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 3 opt

<400>  9345
gggagaaagc uuaccauguc acaaaaagua auauaucuag uaaugauauu auuaauagca       60

ccggcauaua gcauaagaug uaucggagua aguaauagag acuuuguaga aggaaugagu      120

ggaggaacau ggguugaugu aguacuagaa cauggaggau gcgugacagu aauggcucaa      180

gauaaaccaa caguagauau agaacuagua agcacaacag uaaguaauau ggcagaaguu      240

agaagcuauu guuacgaagc aacaauaagu gauauugcaa gugacaguag auguccaaca      300

caaggagaag cauaucuaga uaaacaaagu gauacacaau auguauguaa aagaacgcuu      360

guagauagag gauggggaaa uggcugugga cuauuuggaa aaggaagccu aguaacaugu      420

gcaaaauucg caugcaguaa aaaaaugaca ggaaaaagua uacagccaga aaauuuagaa      480

uacagaauaa ugcuaagugu acauggauca caacacagug gaaugauagu aaacgauaca      540

ggacaugaaa cagaugaaaa cagagcaaaa guagaaauaa caccgaauag cccgagggcu      600

gaagcaacau uaggaggauu uggaagcuua ggauuagauu gugaaccaag aacaggauua      660

gauuuuagug acuuauacua uuuaacaaug aauaauaaac acugguuagu acauaaagaa      720

ugguuccaug acauaccccu accuuggcau gcaggugcag auacaggaac accacauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcucaug cuaaaagaca aacagcaguu      840

guacuaggau cacaagaagg agcaguacau acagcacucg caggggcauu agaagcagaa      900

auggacggag caaaaggcag auuaucaagc gggcauuuaa aauguagacu aaaaauggau      960

aaauuaagac uaaaaggugu gucauauuca cuauguacag cugcauucac auuuacaaaa     1020

auaccagcag aaacauuaca cggaacgguc acagucgaag uccaauacgc aggaacagau     1080

ggaccaugua aaguacccgc acagauggca guagauaugc aaacacuuac accaguaggu     1140

agauuaauaa cagcaaaucc aguaauaaca gaaaguacag aaaacaguaa aaugaugcua     1200

gaauuagauc caccauuugg agauucauau auaguaauug gagugggcga aaaaaaaauu     1260

acacaucacu ggcaucgaag uggaucaaca auaggaaaag cauuugaagc aaccguaagg     1320

ggagcuaaaa ggauggcagu cuuaggagac acagcuuggg auuucgguuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9346
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 3 opt

<400>  9346
gggagaaagc uuaccaugag ucaaaaagua auauaucuag ugaugauauu auuaauagca       60

ccagcauaua gcauaagaug uauaggagug ucaaacagag auuucguaga aggaauguca      120

ggaggaacau ggguagaugu gguacuagaa cauggagguu gcguuaccgu aauggcacaa      180

gauaaaccaa caguagacau agaauuagua acaaccaccg uaaguaauau ggcagaaguu      240

agaaguuauu guuaugaagc aagcauaagc gacauggcaa gcgacagcag auguccaaca      300

caaggagaag cauauuuaga uaaacaaagc gacacacaau auguaugcaa aagaacuuua      360

guagauaggg guuggggaaa uggcugugga cuauuuggaa aagguaguuu aguaacaugu      420

gcaaaauuug cauguucaaa gaaaaugacu ggaaaaucaa uacaaccaga aaauuuagaa      480

uaucguauaa ugcuaagugu acauggauca caacacagug gaaugauagu aaaugauaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa caccaaacuc acccagagcu      600

gaagcaacgu uaggaggauu uggaucauua ggauuagauu gugaaccaag gacaggauua      660

gauuuuagug aucuauauua ucuaacaaug aauaauaaac acugguuagu ucauaaagaa      720

ugguuucaug auauaccauu accauggcau gcaggagcag acacaggaac accacauugg      780

aacaauaaag aagcgcuagu ggaauuuaaa gaugcacacg caaaaagaca aacuguaguu      840

guacuaggaa gucaagaagg ggcaguacau acggcucuag cuggagcacu ggaagcagaa      900

auggacgggg cuaaagguag auuaucgagc ggacauuuaa aauguagguu gaaaauggac      960

aaacuuagau uaaaaggugu aucauauagu uuauguacag cagcguuuac guuuaguaaa     1020

auaccugcag aaacauuaca cggaacagua acagaagaag uacaauacgc aggaacggau     1080

ggucccugca aaguuccagc acaaauggcu guggacaugc aaacauuaac accuguagga     1140

agauuaauaa cagcaaaccc aguaauaaca gaaagcacag aaaauaguaa aaugauguua     1200

gaauuagauc caccauuugg agauaguuau aucguaauag gaguaggaga aaagaaaauc     1260

acucaucacu ggcauagaag cgguaguaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu acuaggagau acagcguggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9347
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 3 opt

<400>  9347
gggagaaagc uuaccauguc acaaaaagua auuuauuuag uuaugauauu acuaauagca       60

ccagcauauu caauaagaug uauaggagug aguaaucgag auuuuguaga aggcaugagu      120

ggaggaacuu ggguagaugu aguuuuagaa cacggcggau gcguaacagu aauggcacaa      180

gauaaaccga cgguagacau agaauuagua acaacaacag uuaguaacau ggcagaagua      240

agaucauauu guuaugaagc aaguauaagu gauauggcua gugauucaag auguccaaca      300

caaggcgaag cauacuuaga uaaacaaagc gauacacaau auguauguaa gagaacauua      360

guagauagag guuggggaaa uggaugugga uuauuuggaa aaggaucacu aguaaccugc      420

gcaaaauuug cuuguucaaa aaaaaugaca ggaaaaucaa uccaaccaga aaaucuagaa      480

uauagaauaa uguuaucagu acaugggagc caacacucgg gaaugauagu aaaugauacu      540

ggccacgaaa cagacgaaaa ucgagcaaaa guagaaauaa caccaaauuc accaagagca      600

gaagcaacac uaggaggauu uggaucauua ggauuagacu gugaaccaag aacuggacua      660

gauuuuucag accuauacua ucuaacaaug aauaauaaac acugguuagu acauaaagaa      720

ugguuucaug acauaccacu gccauggcau gcaggcgcag auacgggaac gccacauugg      780

aacaauaaag aagcauuagu ugaauuuaaa gaugcacaug cgaaaagaca aacuguagua      840

guauuaggaa gccaagaagg agcaguucac accgcauuag cuggagcguu agaagcagaa      900

auggacggag caaaagggag auuaucaagc ggucaucuaa aaugcagacu aaaaauggau      960

aaauuaagau uaaaaggagu aagcuauucg cuauguacag cagcauuuac auuuacaaaa     1020

auaccagcag aaacauuaca uggaacagua acaguagaau uacaauaugc uggcacagau     1080

ggaccaugua aaguaccagc acaaauggca guagauaugc aaacucuuac accaguagga     1140

cgauuaauua cagcaaaucc aguaauaacu gaaucuacug aaaacaguaa aaugauguua     1200

gaauuagauc caccauuugg agauaguuau auaguaauag gaguaggaga aaaaaaaaua     1260

acacaucauu ggcauagauc agguaguaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu uuuaggugau acagccuggg auuuugguuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9348
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 3 opt

<400>  9348
gggagaaagc uuaccaugag ucaaaaagug aucuauuuag uaaugauucu acuaauagca       60

ccagcauauu caauaagaug uauaggcaua ucuaacaggg auuuuguuga aggaaugagu      120

ggaggaacuu ggguagacgu aguauuggaa cauggaggau guguaacagu aauggcacaa      180

gauaaaccua caguagacau agaacuggua acaacaacag uaaguaauau ggcugaagua      240

aggucauacu guuacgaagc aaguauaagc gauauggcaa gugauucaag augcccaaca      300

caaggagaag cauaucucga uaaacaaagc gauacacaau auguauguaa aagaacauua      360

guagauagag gauggggaaa cgguugcgga uuauuuggga aaggaucuuu aguaacaugu      420

gcaaaauucg cauguaguaa aaaaaugaca ggaaaaucca uacaaccaga aaacuuagaa      480

uauagaauaa uguuaagugu acacgguagu caacauucag gaaugauugu aaaugauaca      540

ggacacgaaa ccgacgaaaa uagagcaaaa guagaaauaa caccaaacuc accaagagca      600

gaagcaacac uaggaggauu uggaauacuu ggauuagauu gugaaccacg aacuggacua      660

gauuuuucag accuauauua ucuaacgaug aauaauaaac auuggcuagu acauaaagaa      720

ugguuucaug auauacccuu accauggcau gcaggcgcag auacgggaac gccacauugg      780

aacaacaaag aagcauuagu agaauuuaaa gaugcacaug caaaacgaca aacaguagua      840

guauuagggu cacaagaagg agcaguacau acugcacuug caggagcuuu agaagcagaa      900

auggacggug caaaaggaag auuaucauca ggacacuuaa aauguagauu aaaaauggac      960

aaauuacgau uaaaaggagu aucauauagc uuaugcaccg cagcauuuac auuuacuaaa     1020

auaccugcug aaacauuaca uggaaccgua acaguagaag uacaauaugc aggaacagau     1080

ggaccaugua aaguaccagc acaaauggca guagauaugc aaacacuaac accaguagga     1140

agacuuauca cagcaaaucc aguaauaaca gaaagcacgg agaauucaaa aaugauguua     1200

gaacuagauc caccauuugg agacucauac auaguaauag gaguaggaga aaaaaaaaua     1260

acacaucauu ggcacagauc aggaaguacu auaggaaaag cauuugaagc aacgguaaga     1320

ggagcaaaga gaauggcagu auuaggagau acagcauggg auuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9349
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 3 opt

<400>  9349
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauauu auuaauagca       60

ccagcauaua guauaagaug uauaggagua aguaauagag auuucguaga aggaaugagu      120

gggggaacau ggguagaugu aguauuagaa cauggagggu guguaacagu aauggcacaa      180

gacaaaccaa ccguggauau ugaauuagua acuacuacag ucaguaauau ggcagaaguc      240

agaucauauu guuacgaagc uaguauauca gacauggcga gugauagcag auguccaaca      300

caaggagaag cauauuuaga uaaacaaagu gauacucaau auguauguaa aagaacauua      360

guugauagag gguggggaaa uggaugugga uuauuuggaa aaggaagcuu aguaacaugu      420

gcaaaauucg cauguaguaa aaaaaugaca ggaaaaagua uacaaccaga aaacuuagaa      480

uauagaauaa ugcugagugu acauggauca caacauucag gaaugauagu aaaugauaca      540

ggacaugaga cagaugaaaa cagagcaaag guagaaauaa cuccaaacag uccacgagca      600

gaagcaacac uagguggauu uggaucacua ggacuagauu gugaaccuag aacaggauua      660

gauuuuucag auuuauauua ucuuacaaug aacaacaaac auuggcuagu acauaaagaa      720

ugguuccacg auauaccacu accauggcau gcuggagccg auaccggcac uccacauugg      780

aacaauaaag aagcauuagu agaauucaaa gaugcacacg cuaaaagaca gacaguggua      840

guauuaggaa gucaagaagg agcaguacac acagcacuag caggagccuu agaagcagaa      900

auggaugggg uaaaagguag guuaagcagu ggacacuuaa aauguagauu aaaaauggau      960

aaacuaagac uuaaaggugu aaguuacuca uuauguacag cagcuuuuac guuuacuaaa     1020

aucccugcag aaacacuaca uggaacagua accguggaag uacaauacgc aggaacagau     1080

ggaccaugua aaguaccagc acaaauggcu guagauaugc aaacauuaac cccaguagga     1140

agauugauaa cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugauguua     1200

gaauuagauc cucccuuugg ugauagcuau auaguuauag guguaggaga aaaaaaaaua     1260

acacaucauu ggcacagauc aggcagcaca auagguaaag cuuuugaagc aacaguaaga     1320

ggagcaaaac gaauggcagu guugggagau acagccuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9350
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 3 opt

<400>  9350
gggagaaagc uuaccaugag ccaaaaagua auuuauuuag uaaugauacu auuaauagca       60

ccagcauaua guauaagaug uauaggggua uccaacagag auuucguuga aggaaugagc      120

ggaggaacau ggguagaugu aguuuuagaa caugguggau guguaacagu aauggcacaa      180

gauaaaccua caguagauau agaauuagua acaacaacag uaucaaacau ggcagaagua      240

agaaguuauu gcuaugaagc aagcauuagu gauauggcaa gugauagucg auguccaaca      300

caaggagaag cauaucuaga caaacaaagu gacacacaau acguauguaa aagaacauua      360

guagauagag gaugggguaa uggcugcgga cuauucggga aaggauccuu aguaacuugu      420

gcaaaauuug ccuguaguaa aaaaaugaca ggaaaaucaa uacaacccga aaaucuagaa      480

uaccgaauaa ugcuaagugu acacggaagu caacauucag gaaugaucgu gaaugacaca      540

gggcaugaaa cugaugaaaa cagagcaaaa guagaaauua caccaaauuc accaagggcu      600

gaagcaacau uaggaggauu cggaaguuua ggacuggauu gugaacccag aacuggauua      660

gacuuuucag auuuauacua uuuaacaaug aauaacaaac acuggcuagu acauaaagaa      720

ugguuucacg auauaccauu accauggcau gcaggagcag auacaggaac accacauugg      780

aauaauaaag aagcacuagu agaauuuaaa gaugcacacg cuaaaagaca aaccguugua      840

guauuaggau cacaagaagg agcgguucac acagcacuag ccggagcauu agaagcggaa      900

auggauggag caaaaggaag auuaucauca ggucacuuaa aauguagguu aaaaauggau      960

aaacuaagac uaaaaggagu aagcuauagu cuuuguacag cagcauucac auucacaaaa     1020

auaccagcag aaacauuaca uggaacagua acaguugaag uacaauaugc aggagcagac     1080

ggaccaugca aaguaccugc acaaauggca guggauaugc aaacauuaac uccaguaggu     1140

agguuaauaa cagcaaaccc aguaauaaca gaaaguacgg aaaauucaaa aaugaugcua     1200

gaauuagauc caccauuugg agauaguuau auaguaauag gaguaggaga aaaaaaaauu     1260

acacaccauu ggcauagauc agguaguaca auaggaaaag cauuugaagc aaccguacgu     1320

ggagcuaaaa gaauggcugu auuaggagau acagcauggg acuuugguag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9351
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 3 opt

<400>  9351
gggagaaagc uuaccauguc acaaaaagua auauaucuag uuaugauacu auuaauagca       60

ccagccuaca guauacgaug uauuggagua aguaauagag auuuuguuga aggaaugagu      120

gggggaacau ggguagaugu gguacuugaa cauggaggau gugucacagu aauggcacaa      180

gauaaaccaa caguggauau agaauuagua acaacuacag uaaguaacau ggcagaagua      240

agaaguuauu gcuacgaagc uucaauaagu gacauggcaa gugauagcag auguccgaca      300

caaggcgaag cauaucuaga uaaacaaagc gauacucaau auguauguaa aagaacauua      360

guagacagag guuggggcaa uggaugugga uuauuuggua aaggcucucu aguaacaugu      420

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaucaa uucaaccaga aaaucuagaa      480

uauagaauaa uguuaucagu ccauggaagu caacauucag guaugauagu aaacgacaca      540

ggacaugaaa cagaugaaaa cagagcaaaa guagaaauca caccaaauag uccaagagcc      600

gaagcaacuu uaggaggauu cggaucauua ggauuagauu gcgaaccaag aaccggguua      660

gauuuuagug auuuauauua ucuuacuaug aauaauaaac auuggcuagu acauaaagaa      720

ugguuucaug auauaccauu accuuggcac acuggagcag auacaggaac accacauugg      780

aacaauaaag aagcuuuagu agaauuuaaa gaugcacaug caaaaagaca aacaguagua      840

guauuaggaa gucaagaagg agcuguacau acugcauuag cuggugcauu agaagcagaa      900

auggauggag caaaaggaag gcuaagcagu ggacaucuaa aauguagauu aaaaauggau      960

aaacuaagau uaaaaggagu aucauauagu cuauguacag cagcauucac auuuacaaag     1020

auaccagcag aaacacuaca cgguacagua acgguagaag uccaauaugc aggaacagau     1080

ggcccuugua aagugccagc acaaauggca guagauaugc aaacacuaac uccaguagga     1140

agacuuauaa cagcaaaucc aguuauaaca gaaggcacag aaaacaguaa aaugauguua     1200

gaacucgauc caccauuugg cgauucauau auaguaauag gaguaggaga aaaaaaaaua     1260

acacaucauu ggcauagguc uggaucaacu auaggaaaag cauuugaggc aacuguaaga     1320

ggagcaaaaa gaauggcagu uuuaggugau acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9352
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 3 opt

<400>  9352
gggagaaagc uuaccauguc acaaaaagua auauauuuag uaaugauauu guuaauagca       60

ccagcguaca guauuagaug uauaggaguu agcaauagag auuuuguaga aggaaugagu      120

ggaggaaccu ggguagaugu aguauuagaa caugggggau guguaacagc aauggcgcaa      180

gauaaaccaa caguagacau agaauuagua acaacuaccg uuaguaauau ggcagaagua      240

cgaagcuauu guuacgaagc uucaauaagu gauauggcaa gugacagcag auguccaaca      300

caaggagaag cguaccuaga uaaacaaagu gauacacaau acguaugcaa aaggacuuua      360

guagauagag gauggggaaa uggauguggu uuauuuggga aaggaagccu uguaacaugu      420

gcaaaauuug cauguagcaa aaaaaugaca gguaaaucaa uacaaccuga aaaucuagaa      480

uauagaauaa uguuaagcgu acauggauca caacauagcg gaauguuagu aaacgauaca      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaauaa cgccaaacuc accucgagcc      600

gaagcaacau uagguggauu uggaucauua ggauuagauu gcgaaccaag aacaggcuua      660

gacuuuagcg auuuauauua cuuaacgaug aacaauaaac auugguuagc ucauaaagaa      720

ugguuucaug auauaccacu accauggcac gcgggagcag caacaggaac gccucauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacaug cuaaaagaca aacaguagua      840

guacuaggaa gccaagaagg agcaguacac acagcauugg caggagcauu ggaagcagaa      900

auggauggag caaaaggacg auuaaguucu ggacauuuaa aaugucgauu aaaaauggau      960

aaacuaagac ucaaaggggu aucauacuca uuauguacag cagcguuuac auucacaaaa     1020

auaccagcag aaacaguaga ugguacagua acaguagaag gacaauaugg uggaacagac     1080

ggaccaugca aagugccagc acaaauggca guagauaugc aaacacuuac accaguagga     1140

agauuaauaa cagcaaaucc agugauaaca gaaucgacag aaaacaguaa aaugauguua     1200

gaacuagauc caccuuuugg agauucauau auuguaauag gaguagggga aaaaaaaaua     1260

acacaucauu ggcauagauc uggaagcacg auaggaaaag cuuuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu uuuaggggau acagcauggg auuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9353
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 3 opt

<400>  9353
gggagaaagc uuaccaugag ccaaaaaguu auauaccuag uaaugauauu auuaauagca       60

ccagccuaua guauacgaug cauuggagua ucaaauagag auuucguaga aggcauguca      120

gguggaacau ggguagaugu aguauuagaa cauggaggau guguaacagu aauggcacaa      180

gacaaaccag caguagauau agagcuagua acaacuacag uaucaaauau ggcagaagua      240

agaaguuacu guuaugaagc uagcauaagc gauauggcau cugauucuag guguccaaca      300

caaggcgaag cauauuuaga uaaacaaucu gauacacaau acgucuguaa aagaacucua      360

guagauagag gauggggaaa cggauguggu uuauucggaa aaggcagcuu aguuacaugu      420

gcaaaauuug caugcucaaa gaaaaugaca ggaaaaucaa uacaaccuga aaaccuagaa      480

uacagaauaa uguuaucagu gcacggauca caacauagcg gaaugauagu caaugauaca      540

ggacaugaaa cagaugaaaa cagagcuaaa gucgaaauaa caccaaacag uccaagagcu      600

gaagcaacau uaggaggauu cgguagcuua ggacuugauu gcgaaccaag aacaggacua      660

gauuuuucag aucuuuauua cuuaaccaug aacaacaaac auugguuggu acacaaagaa      720

ugguuucaug auauaccacu accuuggcau gcaggagcag auacuggaac accacauugg      780

aacaauaaag aagcauuagu agaauuuaaa gacgcucaug caaaaagaca aacagucguu      840

guauuaggau cacaagaagg agcaguacau accgcucuag cuggagcauu agaagcagaa      900

auggauggag caaaaggaag auuaucauca ggacacuuaa aaugcagacu aaaaauggac      960

aaauuaagac ucaaaggagu aucuuauuca uuaugcaccg cagcauuuac auuuacaaaa     1020

auaccagcag aaacacuaca uggaacugua acagucgaag uccaauaugc aggaacagau     1080

ggaccgugca aagucccggc acaaauggca guagacaugc aaacuuuaac accaguagga     1140

cgauuaauaa cugcaaaucc uguaauaaca gaaagcacag aaaauucaaa aaugaugcua     1200

gaauuagauc cacccuucgg agauucauau aucguaauag gaguaggaga aaagaaaaua     1260

acacaucauu ggcauagauc aggaagcacu auagguaaag cauuugaagc aacaguaaga     1320

ggugcaaaaa gaauggccgu uuuaggugau acagcauggg auuuuggcag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9354
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 3 opt

<400>  9354
gggagaaagc uuaccaugag ucaaaaagua auauauuuag ugaugauacu auuaauagca       60

ccagcauaua guauaagaug uauaggugug aauaauagag auuuuguuga aggaauguca      120

gggggaacau ggguagaugu cgucuuagaa cacggagguu guguaacagu gauggcacaa      180

gauaaaccaa caguagauau agaauuagua acaaccacag uaaguaauau ggcagaagua      240

agaucauauu guuaugaggc aaguauaagu gauauggcaa gugauaguag auguccgaca      300

cagggagaag cauauuuaga uaaacaaagc gauacucaau auguauguaa aagaacauua      360

guagauagag gauggggaaa uggaugcgga uuguuuggaa aaggaagucu agugacaugu      420

gcaaaauuug caugcuccaa aaaaaugaca ggaaaaucaa uacaaccaga gaacuuagaa      480

uauagaauaa uguuaagugu acauggaagu caacacagcg gaaugauagu uaaugauaca      540

ggacaugaaa cagaugaaaa ucgagcgaaa guugaaauaa cuccaaacuc accuagagca      600

gaagcaacau uaggaggguu cggaucauua ggacuagauu gugaaccaag aacaggacua      660

gauuucagug acuuauauua ucuaacaaug aauaauaaac auuggcuagu acauaaagaa      720

ugguuucaug auauacccuu accauggcau gcgggugcag auacagguac accacauugg      780

aauaauaaag aagcccuugu ggaauuuaaa gaugcacacg caaaaagaca aacaguaguc      840

guacuagguu cacaagaagg agccgugcac acagccuuag caggagcacu ggaagcagaa      900

auggaugggg caaaaggaag auuaucgagu ggacaucuaa aaugcagacu aaaaauggau      960

aaauuaagau uaaaaggagu aaguuauuca uuauguacag cagcauuuac auuuacaaaa     1020

auaccagcag aaacauuaca uggaacagua acaguugaag uacaauaugc gggaacagac     1080

ggaccaugua aaguaccagc acaaauggca guagacaugc aaaccuuaac cccagucgga     1140

agacuaauaa cagcaaaccc gguaauaacc gaaaguacag aaaacucuaa aaugauguua     1200

gaacuagauc caccauuugg agauaguuac auaguaauag gagugggaga aaagaaaaua     1260

acacaucacu ggcauagaag cggaagcaca auuggcaaag cauucgaagc aaccguaaga     1320

ggagcaaaaa gaauggcagu auuaggagau acugcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9355
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 3 opt

<400>  9355
gggagaaagc uuaccauguc acaaaaagua auauauuuag ucaugauauu gcuaauagca       60

ccugcauaua guauaagaug uauaggagua aguaauagag acuuuguaga aggaaugagc      120

ggcggaacau ggguagacgu aguacuagaa cauggaggau guguaacagu aauggcacaa      180

gauaaaccaa caguagauau agaauuagua acaacaacag uaucuaacau ggcugaagua      240

agaaguuauu gcuacgaagc aaguauaagc gauauggcau cagauuccag auguccgaca      300

caaggagaag cauauuuaga uaaacaaagc gauacacaau augugugcaa aagaacauua      360

guagauagag gauggggaaa uggaugcgga cuauuuggaa aagguagcuu aguaacaugu      420

gcaaaauuug cauguucuaa aaaaaugaca ggaaaaagca uacaaccaga aaauuuagaa      480

uaucgaauaa uguuaagcgu acauggauca cagcauucag gaaugauagu aaacgauaca      540

ggucaugaaa cagacgaaaa uagagcaaaa guagaaauaa caccuaacuc cccaagagca      600

gaagcaacau uaggaggauu uggaucacua ggauuagauu gugaaccaag aacuggacuu      660

gacuucagug acuuauauua uuuaacaaug aauaauaaac auugguuagu acauaaagaa      720

ugguuucacg acauaccauu accauggcau gcaggagcag auacaggaac uccccauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaaagaca aacaguagua      840

guacuuggaa gucaagaagg agcaguacau acagcuuuag caggagcacu ggaagcagaa      900

auggacggug cuaaaggaag acuuucaagu ggacaucuaa aaugucgguu aaaaauggau      960

aaauuaagau uaaaaggcgu aaguuauagc uuaugugcag cagcauuuac uuuuacaaaa     1020

auaccugcag aaacacuaca cgguacaguu acgguagaag uccaauaugc uggaacggau     1080

ggaccaugua aaguaccugc acaaauggca guagauaugc agacauuaac uccaguggga     1140

agauuaauca cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugaugcua     1200

gaauuagauc caccauucgg ggauaguuau auaguaauag gaguagguga aaaaaaaaua     1260

acacaucacu ggcauagauc cggaucgaca auaggaaaag cauuugaagc aacaguaaga     1320

ggggcuaaaa gaauggcagu auuaggagau acagcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9356
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 3 opt

<400>  9356
gggagaaagc uuaccauguc acaaaaagua auauaccuag uaaugauauu auuaauagca       60

ccagcauaua gcauaagaug uauaggagua aguaauagag acuuugucga aggaaugagu      120

ggaggaacau ggguagacgu aguauuagaa cauggaggau guguaacagu uauggcacaa      180

gauaaaccaa caguagacau agaacuagua acaacuacag uuucaaauau ggcugaagua      240

agaaguuauu guuacgaagc aaguauuagc gauauggcga gugauucaag augcccaacu      300

caaggagaag cauaucuaga uaaacaaagu gauacacaau auguauguaa aagaacacua      360

guagacagag gauggggaaa uggaugugga uuauuuggaa aaggaucauu aguuacaugu      420

gcaaaauuug cauguucaaa aaaaaugaca ggaaaaagua uacaaccuga aaauuuggaa      480

uauagaauaa ugcugagugu acauggauca caacacagug gaaugauagu caaugauauu      540

ggacaugaaa cagaugaaaa ucgagcaaaa guagaaauaa caccgaauag cccaagagca      600

gaagcaacuu uaggaggauu uggaucauua gggcuagauu gugaaccuag aacuggacua      660

gauuuuagug acuuguauua cuuaacaaug aauaacaaac acuggcuggu acauaaagaa      720

ugguuucaug auauaccauu accauggcac gcaggugcag acacaggaac accacauugg      780

aauaauaaag aagcauuagu agaauucaaa gaugcacaug caaaaagaca aacaguggua      840

guauuaggaa gucaagaagg agcaguacau acugcauuag caggugcuuu agaagcagaa      900

auggauggag caaaaggaag auuaucuuca ggacauuuaa aaugcagauu aaaaauggac      960

aaauuaaggu uaaaaggagu aaguuacagc cuuuguacag cagcauuuac uuuuacgaaa     1020

auccccgcag aaacuuuaca uggaacagua acaguagaag uacaauaugc aggaacagac     1080

ggaccaugca aaguaccagc acaaauggca gucgauaugc aaacauuaac accuguagga     1140

agacucauaa cagcgaaucc gguuauuacu gaaucaacag aaaauaguaa aaugaugcua     1200

gaauuagacc caccauuugg agauucguau auaguaauag gaguaggcga uaaaaaaaua     1260

acacaucauu ggcauagauc aggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu auuaggagau acagcauggg acuuuggauc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9357
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 3 opt

<400>  9357
gggagaaagc uuaccauguc gcaaaaagua auauauuuag uaaugauucu auuaauagcu       60

ccugcauauu caauaagaug uauaggugua agcaauagag auuuuguaga aggaaugagu      120

ggaggaacgu ggguagaugu aguauuagaa cacggaggau guguaacugu aauggcacaa      180

gauaaaccaa cuguagauau agaauuagua acaacuacag uaucaaauau ggccgaagua      240

agaagcuauu gcuaugaagc uaguauaagu gacauggcau cagacucaag auguccaaca      300

caaggcgaag cauaccuaga uaaacaaagu gauacacaau augucugcaa aaggacgcua      360

guagauagag gauggggaaa uggauguggu cuauuuggaa aagggucauu agucacaugu      420

gcaaaauuug cauguucaaa gaaaaugaca ggaaaaucaa uacagccaga aaauuuagaa      480

uauagaauua uguuaagugu acacggaagc caacacucag gaaugauugu aaacgacaca      540

ggacaugaaa ccgaugaaaa cagagcaaaa guagaaauaa caccuaauuc accaagagca      600

gaagcaacgu uaggaggauu ugguucuuua ggacuagauu gugaaccaag gacaggacua      660

gauuuuagug acuuauauua cuuaacaaug aacaauaaac auuggcuagu ucauaaagaa      720

ugguuucaug auauaccucu accauggcac gccggagcag auacuggaac cccacauugg      780

aauaauaaag aagcauuagu agaauuuaaa gaugcacacg caaaaagaca aacuguagua      840

guauuagguu cucaagaagg ugcaguacac acagcuuuag caggagcauu agaagcagaa      900

auggauggug caaaaggaag acuaaguagu ggacacuuaa aauguagauu aaaaauggau      960

aaacuaagau uaaaaggagu aucauauuca uuauguacug cagcauucac auucacaaaa     1020

auuccagcag aaacuuuaca uggaacaguu acaguugaag uacaauaugc aggaacagau     1080

ggaccaugca aaguaccagc gcaaauggca guagauaugc aaacauuaac uccaguagga     1140

cgauuaauaa ccgcaaaucc aguaauaaca gaaaguacag aaaacaguaa aaugaugcuu     1200

gaauuagacc caccauuugg agauucauau auuguaauag guguaggaga caagaaaaua     1260

acacaccacu gguacagauc gggaucaacg auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaaa gaauggcagu auuaggagac acagcauggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9358
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 3 opt

<400>  9358
gggagaaagc uuaccauguc acaaaaagug auauauuuag uaaugauauu acuaauagca       60

ccagcauaca guauaagaug uauaggagua ucuaauagag auuuuguaga aggaauguca      120

ggaggaacau ggguagaugu aguacuagaa cacggaggau guguaacagu aauggcacaa      180

gacaaaccaa caguagauau agaauuagua acaacaacug uaagcaacau ggcagaaguu      240

agaaguuauu guuaugaagc aaguauaagu gauauggcaa gugauucaag auguccuaca      300

caaggagaag cauacuuaga caaacaaagc gauacacaau auguuugcaa aagaacguua      360

gucgaccgag gaugggggaa cggaugugga cuauuuggaa aaggaagucu aguaacaugu      420

gcuaaauuua cauguagcaa aaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa      480

uacagaauaa uguuaagugu gcauggauca caacauagcg gaaugauagu uaaugauacu      540

gggcacgaaa cugaugaaaa uagagcgaaa guagaaguca caccaaacag uccaagagcg      600

gaagcaacuu uaggaggauu cgguucauua ggacuagauu gcgaaccuag gacuggauua      660

gauuuuucag aucuauauua ucuuacaaug aauaauaaac auugguuagu acauaaagaa      720

ugguuucaug auauaccacu accguggcau gcaggagcag auacaggaac accucauugg      780

aauaacaaag aagcauuagu agaauucaaa gaugcacaug caaaacgaca aacgguagua      840

guacuuggaa gucaagaagg agcaguacac acagcauuag caggagcuuu agaagccgag      900

auggacgggg caaaaggcag acuauuuagc ggacacuuaa aaugcagauu aaaaauggau      960

aaauuaagau uaaagggagu aaguuauuca uuauguacag cugcauuuac uuuuacaaaa     1020

guuccagcag agacauuaca uggaacagua acaguggaag uacaauaugc aggaacagau     1080

gggccaugua aaguuccagc acaaauggca guagauaugc aaacauuaac uccaguagga     1140

agacuuauua cagcaaaucc aguaauaaca gaaaguacag aaaauaguaa aaugaugcuu     1200

gaauuagacc caccauuugg ggauucuuau auagucauag gaguuggaga uaaaaaaaua     1260

acucaucauu ggcauagauc uggcucaacu auaggaaaag cguuugaagc aacgguaaga     1320

ggagcaaaaa gaauggcugu acuaggcgac acagcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9359
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 3 opt

<400>  9359
gggagaaagc uuaccauguc acaaaaaguu auauauuuag uaaugauauu guuaauagca       60

ccggcauaua guauaagaug uauaggagua ucaaauagag auuuugucga aggaaugagu      120

ggaggaacau ggguugaugu aguauuagaa cauggcggau gcguaacugu aauggcacaa      180

gauaaaccaa cuguggauau agaauuagua acaacaacag uaagcaauau ggcagaagua      240

agaaguuauu guuaugaagc aucaauaagu gauauggcaa gugauucucg augcccgaca      300

caaggagaag cauacuuaga uaaacaaagu gauacacaau auguaugcaa gcgaacacuu      360

guagacagag gauggggaaa cggauguggu uuauucggua aaggaaguuu aguaacuugu      420

gcaaaauuua cauguagcaa aaaaaugaca ggaaaaucaa uacaaccuga gaauuuagaa      480

uauagaauua ugcuaagcgu acacggguca caacauagcg gaaugauagu aaaugauaua      540

ggacaugaaa cagaugaaaa uagagcaaaa guagaaguaa cuccaaauag uccaagagca      600

gaagcaacau uagggggcuu cgguucauua gguuuagacu gcgaaccacg gacuggacua      660

gacuucucag auuuauauua uuuaacaaug aauaacaaac acuggcuggu acauaaagaa      720

ugguuucacg auauaccuuu accauggcau gcgggcgcag auacaggaac accucauugg      780

aauaacaaag aagcacuagu agaauuuaaa gaugcacaug caaaacgaca aacaguagua      840

guauuaggaa gucaagaagg ggcaguacau acagcacuag cgggagcauu agaggcagaa      900

auggauggag caaaaggaag guuauucagc ggacauuuaa aauguagacu uaaaauggau      960

aaauuaagau uaaaaggagu aagcuauagu uuauguacag cagcauuuac auuuacaaaa     1020

guaccagcag aaacauuaca uggcacagua accguagaag uacaauaugc aggaacagau     1080

ggaccaugca aaguaccagc ucaaauggcu guagauaugc aaacacuaac accuguagga     1140

agacugauua cagcuaaucc agucauaaca gaaaguacgg aaaauagcaa aaugauguua     1200

gaacuagauc caccauuugg ggauagcuac auaguaauag gaguaggaga uaaaaaaaua     1260

acacaucacu ggcauagauc aggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggagcaaaac gcauggcagu auuaggggac acagcuuggg auuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9360
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 3 opt

<400>  9360
gggagaaagc uuaccaugag ucaaaaaguu auauauuuag uuaugauauu acuaauagca       60

ccagcauauu caauaagaug uauaggagua agcaacagag auuuuguuga aggaaugagc      120

gguggaacau ggguagacgu aguauuagaa cauggaggau guguaacagu aauggcacaa      180

gauaaaccaa caguagacau agaacuagua acaacaacag uuucaaauau ggcagaagua      240

agaaguuauu gcuaugaagc aaguauaagu gacauggcaa gugauaguag auguccaacu      300

caaggugaag cauacuuaga uaaacagagu gacacacaau acguauguaa acgaacucua      360

guagacagag gcuggggaaa uggauguggg cuuuucggaa aaggaucauu aguuacgugu      420

gcaaaauuua caugcaguaa aaaaaugaca ggaaaaucaa uacaaccaga aaaucuggaa      480

uauagaauaa uguuaagcgu acauggaagu caacauucag gaaugauagu aaacgauauc      540

ggacaugaaa cagaugaaaa cagagcaaaa guagaaguaa cuccaaauag cccaagagca      600

gaagcaacau uaggaggauu uggaucauua ggucuagauu gugaaccaag aacaggacua      660

gauuuuagug aucuauacua ucuaacaaug aauaauaaac auugguuagu acacaaagaa      720

ugguuccaug auauaccauu acccuggcau gcaggagcag acacaggaac accacauugg      780

aacaauaaag aagcauuagu agaauuuaaa gacgcacaug caaaaagaca aacuguagua      840

gugcuaggaa gucaagaagg agcuguacac acagcacuag caggugcauu agaggcagag      900

auggauggag caaaaggaag auuauuuagu ggacaucuaa aguguagacu aaaaauggac      960

aaauuaagau uaaaaggagu aaguuauagu cugugcacag cuguaugcac agcagcaaaa     1020

guaccagcag aaacuuuaca uggaacagua acuguagaag uacaauaugc agggacagau     1080

gguccuugua aaguaccagc ucaaauggca guagacaugc aaacgcuaac accaguagga     1140

agacuaauca cagcaaaucc aguuauaacc gaaaguacag aaaauagcaa aaugauguua     1200

gaacucgauc cuccauuugg agauagcuau aucguaauag gaguaggaga caaaaaaaua     1260

acucaucauu ggcauagaag uggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggugcaaaaa gaauggcagu acuaggagac acagcauggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9361
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 3 opt

<400>  9361
gggagaaagc uuaccauguc acaaaaagua auauacuuag uuaugauauu acuaauagca       60

ccggcuuaua guauaagaug cauaggagua agcaauagag auuuuguaga aggaaugagc      120

ggaggaacau ggguagaugu aguauuagaa cacggaggcu gcguaacagu aauggcucaa      180

gauaaaccaa cuguagauau agaacuagua acaacuacag uaucaaauau ggcagaggua      240

agaaguuauu guuaugaagc aucaauaagc gauauggcau cagacaguag auguccaaca      300

caaggugaag cauauuuaga uaaacaaagu gauacacaau auguauguaa aagaacccua      360

guagacaggg gauggggaaa uggcugugga uuauuuggaa aaggaucacu aguuacaugu      420

gcaaaauuca cauguagcaa aaaaaugaca ggaaaaucaa uacaaccaga aaacuuagaa      480

uacagaauaa uguuaagugu acacggaagc caacauucag guaugauagu aaaugaugaa      540

aacagagcaa aaguagaagu uacaccaaau ucaccaagag cagaagcaac acuaggagga      600

uuugguucuc uuggauuaga uugugagcca agaacagguc uggauuuuag ugacuuauau      660

uacuuaacaa ugaauaauaa acauugguua guucacaaag aaugguucca ugauauuccc      720

uuaccauggc auucaggagc agauacagaa acaccacauu ggaauaauaa agaagcauua      780

guagaauuua aagaugcaca ugcaaaaaga caaacaguug uuguacuggg aagccaagaa      840

ggggcaguac auacagcauu agcuggagca cuagaagccg aaauggaugg agcgaaaggc      900

agacuaucaa guggacauuu aaaauguaga uuaaaaaugg auaaauuaag auuaaaagga      960

guaagcuacu cauuauguac agcagcauuc acauuuacaa aaguaccagc agaaacauua     1020

caugguacag uaacaguaga aguccaauau gcuggaagag auggaccaug uaaaguacca     1080

gcacaaaugg caguagauau gcaaacauua acaccaguug guagauuaau aacagcuaau     1140

ccagugauaa cugaaucaac agaaaauagu aagaugaugu uagaacuaga uccaccauuc     1200

ggagacaguu auauaguaau aggagucgga gauaaaaaaa uaacgcauca uuggcauaga     1260

agcggaagua uuauaggaaa agcauucgaa gcaacaguaa gaggagcaaa aagaauggcg     1320

guacuaggag auacagcuug ggauuuuggu aguugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  9362
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 3 opt

<400>  9362
gggagaaagc uuaccauguc gcaaaaagua auauaucucg uuaugauagu ucuaauagua       60

ccagcuuaca guauaagcug uauaggagua ucaaauagag acuuagugga aggcaugagc      120

ggagguacau ggguagacgu aguauuagaa cacggaggau gcguaacaga aauggcccaa      180

gauaaaccaa cuguggauau agaauuagua acgaugacag uaagcaauau ggcugaggua      240

agaagcuauu guuacgaagc aaguuuauca gauauggcua gcgcaucaag guguccaaca      300

caaggcgaac caucauuaga uaaacaaagu gauacucaaa guguauguaa aagaacucua      360

ggagauaggg gauggggaaa uggaugcgga auauucggua aaggaagucu aguaacaugc      420

aguaaauuca cauguuguaa aaaaaugccu ggaaaaucaa uucaaccaga aaauuuagaa      480

uacagaauaa ugcuaccagu acauggaagu caacauagcg gaaugauagu uaaugauaua      540

ggacaugaaa cugaugaaaa cagagcaaaa guagaaguaa cuccaaacag cccuagagca      600

gaagcaacac ucggaggauu cggaucacuc ggauuagauu gugaaccaag aacaggacuu      660

gacuucagug aucuuuacua uuuaacgaug aauaacaaac auugguuagu acauaaagaa      720

ugguuccaug auauaccauu accauggcau gcaggagcag auacaggaac accacauugg      780

aacaauaaag aagcguuagu agaauuuaaa gaugcacaug caaaaagaca aacaguagua      840

guauuaggaa gccaagaagg agcuguacac acggcacuag caggggcauu agaagcagaa      900

auggauggag ccaaagguag auuauuuagu ggacauuuaa aauguagauu aaaaauggau      960

aaacuaagac uaaaaggagu aaguuacagc uuauguacag cagcauuuac auucacaaag     1020

guaccagccg aaaccuuaca cggaacaguu acaguagaag uccaaagugc aggcacagau     1080

gguccaugua aaguaccggc acaaauggca guagauaugc aaacuuuaac accuguagga     1140

agguuaauaa cagcaaaucc aguaauaaca gaaagcacag aaaauaguaa aaugaugcua     1200

gaauuagacc caccauuugg agauucauau auaguaauag gagucggaga uaaaaaaaua     1260

acacaucauu ggcacagaag uggaucaaca auaggaaaag cauuugaagc aacaguaaga     1320

ggcgccaaaa gaauggcagu auuaggagau acagcauggg auuuuggauc uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9363
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 3 opt

<400>  9363
gggagaaagc uuaccaugag acaaaaagua auauauuuag uaaugauauu acuaauugca       60

ccugcauauu caauaagaug cauagguguu aguaauagag auuuuguuga aggaauguca      120

ggaggaacuu ggguagacgu aguacuagaa caugguggcu guguaacagu aauggcacaa      180

gauaaaccaa caguagauau agagcuaguu acgacaacag uuagcaacau ggcagaggua      240

agaaguuauu guuaugaagc aaguauauca gacauggcaa gcgauucaag guguccaaca      300

caaggugaag cauacuuaga uaaacaaagu gauacacaau acguauguaa aagaaccuua      360

guugauagag guuggggaaa ugguugugga cuauuuggaa aaggaaguuu aguuacaugc      420

gcaaaauuug cauguaguaa aaaaaugaca ggaaaaucaa uacaaccaga gaauuuagaa      480

uauagaauaa ugcuuagugu acauggaagu caacauagcg gaaugauagu aaaugacaca      540

ggacaugaaa cugaugaaaa uagagcaaaa guagaaauaa caccaaauuc acccagagca      600

gaagcuacau uaggaggauu uggaucauua ggauuagacu gugaaccuag aaccggauua      660

gauuuuucag aucuauauua cuuaacaaug aauaauaaac auugguuagu acauaaagaa      720

ugguuucaug auauaccguu accauggcau gcaggcgcag auacaggaac cccacacugg      780

aauaauaaag aagcacuagu agaauuuaaa gaugcccaug caaaacguca aacuguagua      840

guacuaggau cacaagaagg agcaguacac acugcauuag ccggagcuuu agaagcagaa      900

auggauggag cgaaaggaag acuaucaucu ggacaccuaa aauguagauu aaaaauggau      960

aaauuaagau uaaaaggagu aaguuauagu uuaugcacag cagcauucac auuuacgaaa     1020

auuccagcag aaacauuaca cggaacagua acaguagaag uucaauaugc gggaacagac     1080

ggaccaugua aaguaccagc acaaauggca guugauaugc agacauugac ucccguugga     1140

agacuaauaa cagcaaaucc aguaauaacu gaaucaacag aaaacucaaa aaugaugcua     1200

gaauuagauc caccauuugg agauucauau auaguaauag gaguagguga aaaaaaaaua     1260

acucaucauu ggcacagaag ugggaguaca auaggaaaag cauuugaagc aacuguaaga     1320

ggggcaaaac gaauggcagu acuaggagau acugcauggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9364
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 3 opt

<400>  9364
gggagaaagc uuaccaugag ucaaagagua auauacuuag uaaugauauu auugauagca       60

ccagcauauu caauuagaug uauaggagua aguaauagag auuuuguaga aggaaugagu      120

ggaggaacau ggguagaugu aguauuagaa cauggaggau guguaacugu uauggcacaa      180

gauaaaccaa caguagauau agaauuagua acaacaacag uaucaaauau ggcagaagua      240

agaagcuacu guuacgaagc aucaaucucu gauauggcaa gugauaguag auguccgaca      300

caaggagaag cuuauuuaga uaaacaaagc gauacacaau auguaugcaa aagaacauua      360

guugauagag gauggggaaa uggaugugga uuauuuggaa aagguagucu aguaacaugu      420

gcuaaauuua cauguagcaa aaaaaugaca ggaaaaagua uacaaccaga aaaucuagaa      480

uauaggauaa ugcuaucagu acacggaagu caacacagcg gaaugauugu aaaugacaua      540

ggacacgaaa cagaugaaaa ucgagcaaaa guagaaguaa cuccaaauuc accuagagca      600

gaagcaaccu uaggagguuu ugggucauua ggacuagacu gugaaccaag aacaggauua      660

gauuuuagug acuuauauua cuuaacaaug aauaauaaac auuggcuagu acacaaagaa      720

ugguuucacg auauaccacu accauggcac gcaggagcag auacuggaac accacauugg      780

aauaauaaag aagcauuggu ugaauuuaaa gaugcacaug cuaaaagaca aaccguagua      840

guguuaggca gccaagaagg agcaguacau acagcuuuag caggagcauu agaagcagaa      900

auggauggag caaaagguag auuauuuuca ggacaucuga aauguagaau aaaaauggau      960

aaauuaagau uaaaaggagu auccuauagu uuauguacag cagcauucac guuuaguaaa     1020

guaccagcag aaacguuaca ugguacagua acaguagaag uacaauaugc agguagggac     1080

ggaccgugua aaguaccagc acaaauggcc guagauaugc aaacauuaac accaguagga     1140

cgauuaauaa cagcaaaccc aauaauaaca gaaucaacug aaaauaguag aaugauguua     1200

gaauuagauc caccauuugg agauucauau auaguuauag guguaggaga uaaaaaaaua     1260

acccaucauu ggcauagaag uggcucaaca auagguaagg cauuugaagc gacaguaaga     1320

ggagcgaaaa gaauggcugu gcuaggagau acugcauggg auuuuggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9365
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  9365
gggagaaagc uuaccaugcg uauaaucaac gcacgaaaag agaagaaaag gcgcggcgcc       60

gacacuucag ucgguaucgu cggccuccuc cucacaaccg ccauggccgc agaagucacc      120

cgccgaggaa gugcauacua cauguaccua gaccgcaaug acgccggaga agcaaucagc      180

uuccccacca cacucgguau gaacaaaugc uacauacaaa uaauggaccu cggucacaug      240

ugcgacgcca cgaugagcua cgaaugcccc augcucgacg aaggggucga accagacgac      300

guugacugcu ggugcaacac aaccucuacu ugggucgucu acgguaccug ccaccacaag      360

aaaggcgaag cgcgacgauc ccgcagggcc gucacccugc cgagccauuc aacccgaaaa      420

cuacaaaccc gaagccaaac cuggcucgaa ucucgcgaau acaccaaaca cuugauccgc      480

gucgaaaacu ggauuuucag aaacccaggc uucgcacucg cggcagccgc aauagccugg      540

cuccucggcu caucaacauc ccagaaaguc aucuaccucg uaaugauucu acucaucgcc      600

ccagcauacu caauacggug cauaggagug uccaaccgcg acuucgucga gggaauguca      660

ggcggcacau ggguagacau aguacucgaa cacggaggau gcgucaccgu aauggcccaa      720

gacaaaccaa ccguugacau agaacucgua accacaaccg uaagcaacau ggcagaaguc      780

cgcucauacu gcuacgaggc auccaucagc gacauggccu ccgacagccg augccccacc      840

caaggagagg ccuaccucga uaaacaaagc gacacccaau acgucugcaa aagaacccuc      900

guagauagag guuggggcaa cggcugcggu cuauucggca aaggaagccu aguaacaugc      960

gccaaauucg ccugcuccaa gaaaaugacc ggcaaaucca uccaaccgga gaaccuugaa     1020

uauagaauca ugcuguccgu acacggaucc caacacuccg gaaugauagu caacgacaca     1080

ggccacgaga ccgacgagaa ccgagcaaaa guagagauca cacccaacuc gccaagagcc     1140

gaagccaccc uagguggcuu cggaucacuu ggccucgacu gcgaaccccg gacagggcuc     1200

gacuucucag accucuacua ccucaccaug aacaacaaac acuggcuugu acacaaagaa     1260

ugguuucacg acaucccacu cccauggcau gcaggcgcag acaccggcac accacacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg caaaacgcca aacaguggua     1380

guccuggguu cccaagaagg cgccguccac acagcccucg ccggcgcccu cgaagcagaa     1440

auggacggcg caaaaggacg ccuuagcucc ggccaccuaa aaugcagacu caaaauggac     1500

aaacuccgcc uuaaaggcgu aucauacagc cucugcacag cugcauucac cuucaccaaa     1560

auacccgccg aaacccucca cggaacaguc accgucgaag uacaauacgc aggaacggau     1620

ggcccuugca aaguaccagc ccagauggcc gucgacaugc agacccuaac accagucgga     1680

agacucauaa ccgccaaucc agucauaaca gaguccaccg aaaacuccaa aaugaugcuc     1740

gaacuagacc cuccauuugg cgacuccuac auagucauag gcguaggaga aaagaaaaua     1800

acacaccacu ggcaccgcuc cgguuccacc auuggaaaag cauucgaagc gacaguacgc     1860

ggggcaaaac guauggcggu gcucggcgac accgccuggg acuuuggguc cgucggcggc     1920

gcccucaauu cacucggcaa aggaauacau caaaucuucg gagcggccuu caaaucccua     1980

uucggcggca ugagcugguu cucccaaaua cugaucggca cacuacucau guggcuaggc     2040

cucaacacca aaaacggcuc aaucucccuc augugccugg cacucggcgg cguacucauc     2100

uuccuaucca cagcagugag ugccgacgug ggaugcuccg uagacuucuc gaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9366
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(93-804) mRNA Design 3 opt

<400>  9366
gggagaaagc uuaccaugcg caucaucaac gcccgaaaag aaaagaaacg acgaggagcg       60

gacacgucag ucggcaucgu aggauuacug cucaccacag caauggccgc cgaagucacc      120

cgcaggggca gcgcauauua cauguaccuu gaccgaaacg acgcggggga agcaauaucg      180

uuccccacca cgcuaggcau gaacaaaugc uacauccaaa uaauggaccu aggccauacc      240

ugcgacgcca caaugucaua cgaaugccca augcuagacg aaggcguaga acccgacgac      300

guagacugcu ggugcaacac aacaagcaca ugggucgucu acgggacaug ccaccacaag      360

aaaggcgaag cucgcagaag cagacgcgcc gucacacugc caagccacuc cacccgaaaa      420

cuacaaaccc gcagccaaac cuggcucgaa agccgcgaau acacaaaaca ccucauacga      480

gucgaaaacu ggaucuuccg aaaccccggc uucgcccugg cagcagcagc cauagccugg      540

cuccuaggcu caagcacaag ccaaaaagua auauaccuag ucaugauccu acuaaucgcg      600

ccagcguacu caauccgaug caucgguguc agcaaccgag acuucgucga aggcauguca      660

gguggcaccu gggucgaugu cguccuggaa cacggcgguu gcgucacggu cauggcccag      720

gauaaaccua ccguagacau agaacugguc accacgaccg uaagcaacau ggcagaagug      780

aggagcuauu gcuacgaagc aucaauaucg gacauggccu cugauucgcg augcccgacc      840

caaggcgagg cauacuugga uaaacaauca gauacccaau acguauguaa acgaacgcua      900

guagaccgag ggugggggaa cgguugcgga cucuuuggaa aaggcucccu aguaaccugc      960

gccaaauucg cguguucaaa gaaaaugaca ggcaaaucaa uacagccaga aaaccucgaa     1020

uaccggauaa ugcucuccgu ccacgggucc caacacagcg gcaugauagu caacgacaca     1080

ggccacgaaa cagacgaaaa uagggccaaa gucgaaauaa caccaaacuc accacgagcg     1140

gaagccacgc ucggcggauu cggaucccua ggccucgacu gugaaccccg gaccggccug     1200

gacuuuagcg accuauacua ccucacaaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuucacg acaucccccu gccauggcac gcgggcgcag acacuggcac accacacugg     1320

aacaacaaag aagcucuugu cgaguucaaa gacgcacacg ccaaaagaca aacagucgug     1380

guacucggau cccaagaagg agccguacac accgcccucg ccggcgcccu cgaagccgag     1440

auggacggug ccaaaggacg ccucagcagc ggacaccuaa aaugcagacu aaaaauggac     1500

aagcucagac ucaagggcgu auccuacucc cucugcaccg ccgccuucac cuucacaaaa     1560

auccccgccg aaacccuaca cgggaccguc accgucgaag uacaguacgc cggcacagau     1620

ggccccugua aaguaccagc ccaaauggcc gucgacaugc aaacccugac acccgucgga     1680

cgccuaauaa cggccaaccc cgucaucacc gaaucaaccg aaaacuccaa aaugaugcuc     1740

gaacuagacc cacccuucgg cgacagcuac aucgucaucg gggucggaga aaagaaaaua     1800

acccaccacu ggcaccgauc cgggucaacc aucggcaaag cauucgaagc caccguccgc     1860

ggcgcaaaac gcauggcggu ccugggagac acagcuuggg acuucggauc cgucggcggc     1920

gcccuaaacu cacucggaaa aggcauucac caaaucuucg gcgccgccuu uaaaagccuc     1980

uucggcggaa ugucgugguu cucccagauc cuuauaggaa cccuacuaau guggcucggc     2040

uugaacgcca aaaacggaag cauaagucug augugccucg cacucggagg cguccuaauc     2100

uuccuaagca ccgcaguaag cgccgacguu ggauguagug uagacuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9367
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(93-800) mRNA Design 3 opt

<400>  9367
gggagaaagc uuaccaugag aauaaucaac gcccgaaaag aacgcaaacg cagaggagcc       60

gacacaagca uaggcauuau cggccuacuu cucaccaccg caauggcugc agaaaucacc      120

agacgaggaa gcgccuauua cauguaucuc gacagaucag augcaggaaa ggcaaucucc      180

uucgccacca cacucggagu aaacaaaugc cacgugcaaa ucauggaccu cggacacaug      240

ugcgacgcga caaugucaua cgagugcccc augcuagacg agggagucga gccagacgac      300

guagacugcu ggugcaacac aaccucaacc ugggucgucu acggaacaug ccaccacaaa      360

aagggggaag cacgccgaag ccgcagagcg gucacacuac caagucacuc cacacgcaag      420

cuccaaaccc ggagccagac cuggcuagaa ucccgggaau acaccaaaca ccuaauaaaa      480

guagagaacu ggauauuucg gaacccagga uucgcgcucg ucgccguagc caucgcaugg      540

cuacuaggcu ccagcacauc acaaaaaguc auauaccuug uaaugauccu ccucaucgcc      600

cccgcauacu ccauccgcug cauaggaguc agcaaucgcg acuucgucga aggcauguca      660

ggaggaaccu ggguagaugu aguacucgag cauggcggcu gcgucacagu cauggcacaa      720

gacaaaccaa ccguagacau cgaacucguc acaaccaccg ugagcaacau ggcugaaguc      780

cggucauacu gcuacgaggc aucaauaucc gacauggccu ccgacagccg cugcccaacc      840

caaggcgaag ccuaccucga caaacagagc gacacacaau acgucugcaa acgaacccuc      900

guagacagag gcugggggaa cggaugcgga cuauucggca aaggaucccu cguaaccugc      960

gccaaguuca cgugcagcaa gaaaaugacc ggcaaaucca uucaacccga aaaccuagag     1020

uaccgcauca ugcuaucagu ccacggcagu caacacucag ggaugaucgg cuacgaaacc     1080

gacgaagacc gcgccaaagu ggaagucaca ccaaacucac cacgcgccga agcaacacua     1140

ggcggguucg gaucccuggg acucgacugc gagccccgca cgggccuaga cuuuagcgac     1200

cuuuacuauc uaaccaugaa caacaaacac uggcuaguac acaaagagug guuccacgac     1260

aucccacuac cguggcacgc aggggcagau accggcacac cgcacuggaa caauaaagaa     1320

gcccuaguag aguucaaaga cgcacacgcc aaacgacaaa ccguaguggu cuuaggaucc     1380

caggaaggag ccguacacac cgcccucgcc ggagcccuag aagccgaaau ggacggcgcc     1440

aaaggccgcc uauuuucggg ccaccucaaa ugccgccuga aaauggacaa acuacgccua     1500

aaagggguaa guuacucccu cugcaccgcc gcauucacau ucacuaaagu cccagccgaa     1560

acccuccacg gcacaguaac aguagaaguc caauacgccg gaaccgacgg acccugcaaa     1620

auccccguac aaauggcagu agauaugcaa acccuaaccc ccgucggccg ccuaauaaca     1680

gccaaucccg uaauuaccga auccacagag aacagcaaga ugaugcucga acuugaccca     1740

cccuucggcg acucguacau agucaucgga gucggcgaca agaaaauuac acaccacugg     1800

caccggucag gaucgacaau cggcaaagcg uucgaggcaa ccguacgagg agccaaaaga     1860

auggccguac uaggugacac cgcgugggac uucggcuccg uagggggcgu auucaacucc     1920

cuaggaaaag gaauccacca gaucuucggc gccgcauuca agucacuauu cggggguaug     1980

agcugguuca gccaaauccu aaucggcacc cuacuggugu ggcucggucu caacaccaaa     2040

aacggaucaa ucucccucac augccuagca uuagggggag ucaugaucuu ccucagcaca     2100

gcagucucag ccgacguggg augcagcgua gacuucucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  9368
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(93-804) mRNA Design 3 opt

<400>  9368
gggagaaagc uuaccaugag aaucaucaac gccaggaaag aaaagaaaag acgaggagca       60

gauaccagug uaggcauagu aggcuuacuc cucacuacag caauggcagc cgaaguaaca      120

agacggggcu ccgcguacua cauguaccuc gaccgaaacg acgcaggaga ggcgaucagc      180

uuccccacga cgcuagggau gaacaaaugc uacauacaaa ucauggaccu cggccacaug      240

ugcgacgcaa caaugagcua cgaaugcccc augcuggaug aaggagugga gcccgacgac      300

guagacugcu ggugcaauac cacaaguacc uggguggugu acggaacaug ccaccacaag      360

aaaggcgaag cccgacgauc gcgccgagcc guaacucugc cgucccauuc gaccagaaaa      420

cuccaaacaa gaucacagac auggcuagag ucacgggaau auacaaaaca ccucauccgc      480

guagaaaacu ggauauuccg aaacccgggc uucgcccucg cagcggcagc aaucgccugg      540

cugcugggga guucgaccag ccaaaaaguc auauaccuag uaaugauccu acucaucgca      600

ccggcuuaca gcauacgcug cauaggaguc ucaaaccgag acuucgucga aggaaugagc      660

ggcggcaccu ggguagacgu aguacucgag cacggcgggu gcguaaccgu cauggcacaa      720

gacaaaccaa caguagacau ugagcuaguc acgacaacag uauccaacau ggcugaagua      780

agaaguuacu guuacgaagc cagcaucagc gacauggcaa gcgacucucg cugccccacc      840

caaggagaag ccuaccucga caaacaguca gacacccagu acguauguaa acgaacgcua      900

guagacagag gcuggggcaa cggauguggc cuuuuuggaa aaggauccuu agucacaugc      960

gccaaauucg ccugcagcaa gaaaaugaca gggaaaucaa uccagccaga aaaccuagaa     1020

uaccggauca ugcucagcgu ccacggcucc cagcacucgg gaaugaucgu aaacgacaca     1080

ggccacgaaa cagacgaaaa ucgcgccaaa guagaaauaa ccccgaacag cccccgcgca     1140

gaagccacuc uaggagguuu cggaagccuc ggucuagacu gcgagccccg caccgggcua     1200

gacuucagug accuauacua ccugacaaug aacaacaaac acuggcuagu ccacaaagaa     1260

ugguuccaug acaucccacu accguggcac gcaggcgcag auaccggcac cccacacugg     1320

aauaacaaag aagcccucgu cgaguucaaa gacgcgcacg ccaaacgcca aacugugguc     1380

guacucggga gucaggaagg cgcaguacac accgcacucg caggcgcccu agaagcagaa     1440

auggacggcg caaaaggacg ccuauccagc ggacaccuca aguguagacu aaaaauggac     1500

aaacuccgac ucaaaggugu aagcuacucc cuaugcacgg cagcauucac cuucacgaaa     1560

auaccagcgg aaacacucca cggaacagua acaguagaag uccaauacgc agggacugac     1620

gggccaugca aaguacccgc gcaaauggca guagacaugc aaacacucac accggucgga     1680

cgacuaauaa cagcaaaccc gguaaucacc gaaagcaccg aaaacagcaa aaugaugcuc     1740

gaacuagacc cacccuucgg agacucauac aucgucaucg gcguaggaga aaagaaaauc     1800

acgcaccacu ggcacagauc aggaucgacu auaggcaaag ccuucgaagc aacagucaga     1860

ggggccaaac gcauggcagu acugggggac acagccuggg acuucggguc cgucggcggg     1920

gcucuaaaca gccuaggcaa agggauacac caaaucuucg gcgcagcauu caaaucacuc     1980

uucggcggca ugagcugguu cagccaaauc cuaaucggaa cacuacucau guggcuaggg     2040

uuaaacacca aaaacggcag caucagccuc augugcuuag cccuaggcgg gguacucaua     2100

uuucuaagca cagcaguaag cgccgacgua ggaugcagcg uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9369
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  9369
gggagaaagc uuaccaugac guccguaggc aucguggggc uccugcuaac gacggccaug       60

gcagcagagg uaacccgccg aggcucagca uacuacaugu accuagaccg aaacgacgcg      120

ggagaagcca ucagcuuccc caccacccuc ggaaugaaca aaugcuacau ccaaauaaug      180

gaccucggcc acaugugcga cgcgacaaug ucauacgaau gcccaauguu agacgaaggg      240

gucgaacccg acgacgucga cugcuggugc aacaccacaa gcacaugggu aguauacggu      300

accugccacc acaagaaagg cgaagccaga cgcagccgca gagccguaac ccucccgucc      360

cacagcacac ggaaacucca gacacggagu caaacauggc uggaaucgcg cgaauacacc      420

aaacaccuua uaagaguaga aaacuggauc uuccgaaacc cgggcuucgc gcucgccgca      480

gccgccaucg cauggcuccu gggguccagc accagccaaa aagucauaua ccucgucaug      540

auccuccuca uagcccccgc cuacuccauc cgcugcaucg guguguccaa ccgagacuuc      600

guagaaggaa ugucgggagg aacaugggua gacauagucc ucgaacacgg cgggugcguc      660

accgucaugg cgcaagacaa accaacagua gacauagagc uaguaacaac aacagucagc      720

aacauggcug aaguccgauc cuacugcuac gaagcaucaa ucagcgacau ggcaucugac      780

agucgaugcc ccacacaagg ugaagccuac cucgacaaac agucagacac acaauacgua      840

ugcaaacgca cacucguaga cagaggcugg ggcaacggau gcggacuguu cggcaaaggc      900

ucacugguaa caugcgccaa auucgcaugc agcaagaaaa ugacaggcaa aagcauccaa      960

ccagaaaacc uagaauaccg caucaugcua agcguccacg gaagccaaca cagcggcaug     1020

auagucaacg acaccgggca cgaaacagac gaaaaccgag ccaaagucga aaucacgcca     1080

aacagccccc gcgccgaagc aacacuaggc ggcuucggaa gccucggccu agacugcgaa     1140

ccacgcacag gacuagacuu cagcgaccua uacuaccuca cuaugaacaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacaua ccccucccuu ggcacgccgg cgccgacacc     1260

gguacacccc acuggaacaa caaagaagcc cucguagagu ucaaggacgc ucacgccaaa     1320

cgacaaaccg ucgucguucu cggaucccaa gaaggcgcag uacacacagc acuagccggc     1380

gcccuagaag cugaaaugga cggagccaaa ggacgccuau caucaggaca uuuaaagugc     1440

cgccucaaaa uggacaaacu ccgacucaaa ggagucuccu acagccugug cacagcagcc     1500

uucacauuca caaaaauacc cgcagagacc cugcacggaa ccguaaccgu agagguccaa     1560

uaugccggaa ccgacggccc cugcaaaguc ccagcccaaa uggcagucga caugcaaaca     1620

cucacccccg uaggaagacu aaucaccgca aacccaguaa ucaccgaaag uaccgaaaac     1680

agcaaaauga ugcuagaacu cgauccccca uucggcgaca gcuacauagu caucgggguc     1740

ggcgaaaaga aaaucaccca ccacuggcac cgcucaggau caacaauagg aaaagcauuc     1800

gaggcaaccg uacgcggcgc caaacgcaug gccguccuag gagacacagc augggacuuu     1860

ggcuccguag gaggcgcgcu caacagccuc ggaaaaggaa uacaccaaau cuucggggcc     1920

gccuucaaau cccuauuugg aggaaugucg ugguucucgc aaauccucau aggcacacuc     1980

cugauguggc ucgggcuaaa caccaaaaac ggcuccauca gucuaaugug ucucgcccuc     2040

ggaggagucc uaaucuuccu aagcacagcu guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9370
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794) mRNA Design 3 opt

<400>  9370
gggagaaagc uuaccaugac cagcgucgga auuguaggac ugcuccuaac gacagcaaug       60

gcagccgaag uaaccagaag aggcuccgca uauuauaugu accucgaccg gaacgacgcc      120

ggagaagcca ucuccuuccc aaccaccuua gguaugaaca aaugcuacau ccaaaucaug      180

gaccucggac acacgugcga cgcaaccaug uccuacgaau gcccaaugcu agacgaaggc      240

guagaaccag acgacguaga cugcuggugc aacaccaccu cgacuugggu aguauacgga      300

accugccacc acaaaaaggg cgaagcacgc cgaucacgca gagccgucac ccuccccuca      360

cacagcaccc gaaaacugca aacacggucc caaaccuggc ucgaaagccg agaauauacu      420

aaacaccuca uaagaguaga gaacuggauu uucagaaacc cgggcuucgc ccucgccgcg      480

gcggcaauag cauggcuccu aggcuccucc acaucgcaaa aagugauaua ucucgucaug      540

auacuacuca ucgccccagc auacuccaua cgaugcaucg gcguauccaa uagagacuuc      600

gucgaaggaa ugagcggagg cacgugggug gacguagucc ucgaacacgg cggcugcgua      660

accgucaugg cacaagauaa accaacggua gacaucgaac ucgucacaac caccguauca      720

aacauggcug aaguccgauc cuacugcuac gaagccucca ucagcgacau ggcauccgac      780

ucacgcugcc ccacccaagg cgaggccuac cuggacaaac aaucagacac ccaauacguc      840

uguaagcgca cccuggugga ccggggcugg ggcaacgggu gcggacucuu cggcaaaggc      900

agccucguaa caugcgcaaa auucgccugc agcaagaaaa ugacaggaaa aagcauccaa      960

cccgaaaacc ucgaauaccg aauaaugcua agcguacacg gaucccaaca cuccgguaug     1020

auaguaaacg acacaggcca cgagaccgac gaaaaccgcg ccaaagucga aauuaccccc     1080

aauucgcccc gagcagaagc uacccuagga ggguuuggcu cccuaggacu ggacugcgaa     1140

ccacgcaccg gacuagacuu cuccgaccua uacuaccuca ccaugaacaa caaacauugg     1200

cuaguacaua aggaaugguu ccacgacauc ccacuacccu ggcacgccgg agccgacaca     1260

ggcacacccc acuggaacaa caaagaggca cucguagagu ucaaagacgc ccacgccaaa     1320

agacaaaccg ucgucguucu cggcucacaa gaaggcgccg uacacacggc ccucgcuggu     1380

gcccuggaag ccgaaaugga cggagccaaa ggacgccucu ccagcggcca ccuaaaaugc     1440

cgccuaaaaa uggacaaacu ccgccucaaa ggcguauccu acucacucug caccgccgcc     1500

uucaccuuca ccaaaauacc cgcagaaacg cuccacggca caguaaccgu cgaaguacag     1560

uacgcuggaa cggauggucc cugcaaagua cccgcccaaa uggcaguaga caugcaaacc     1620

cucacccccg uaggccgccu caucaccgcc aacccaguaa ucacagaauc aaccgaaaac     1680

ucgaaaauga uguuagaacu cgacccaccc uucggagacu cauacaucgu caucggcguc     1740

ggcgagaaaa agaucacgca ccacuggcac cgcagcggcu caacaaucgg aaaggccuuc     1800

gaagcaaccg uaagaggagc caaacgcaug gccguacucg gcgacaccgc augggacuuc     1860

ggcuccgucg gcggagcacu aaacucccuc ggcaaaggaa uccaccaaau cuuuggcgca     1920

gcuuuuaaau cccucuucgg cggcauguca ugguucucuc aaauccucau cggcacacuc     1980

cuaauguggc ucggguuaaa cgcuaaaaac ggcuccauca gccucaugug ccuagcacuc     2040

ggcggaguac uaauauuccu aucaaccgca guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9371
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790) mRNA Design 3 opt

<400>  9371
gggagaaagc uuaccaugac auccauaggc aucauaggau uacuacuaac caccgccaug       60

gccgccgaaa uaacacgccg gggauccgcu uacuauaugu accucgaccg cagugacgcc      120

ggcaaagcua ucagcuucgc aaccacucua ggaguaaaua aaugccacgu ucaaaucaug      180

gaccuaggcc acaugugcga cgccacaaug uccuacgaau gccccaugcu agacgaaggu      240

guagagccgg acgacgucga cugcuggugc aauaccacca gcaccugggu cgucuacggc      300

acgugccacc acaagaaagg agaagcacgu cguucccgac gcgccguuac ucugccaagc      360

cacuccaccc guaaacucca gaccagauca caaacauggc uagaguccag agaauacacc      420

aaacaccuca ucaaagucga gaacuggaua uuccgaaacc ccggcuucgc ccucgucgcc      480

gucgcaaucg ccuggcuacu cggauccagc acuucacaga aagucaucua ccucgucaug      540

auccuacuca ucgccccggc uuacagcauc cgaugcaucg gagugagcaa ccgugacuuc      600

gucgaaggca ugucuggcgg uaccugggua gacgucgucc uagaacacgg aggcugcguc      660

accguaaugg cccaagacaa acccacuguc gacauagaac ucgucacaac aaccgucagc      720

aauauggcag aaguccgcuc cuacugcuac gaggccucca uaagcgacau ggcaucagac      780

ucccgcugcc caacccaagg agaagccuac cuggacaaac aauccgacac gcaauacguc      840

ugcaaaagaa ccuuagucga ccgcggaugg ggaaacgggu gcggccuauu cggcaaaggc      900

ucgcucguaa ccugcgccaa guucacaugc agcaaaaaga ugacgggcaa aagcauacaa      960

ccggaaaacc ucgaauacag aauaaugcuc ucgguccacg gcucccaaca cagcggaaug     1020

auuggauacg aaacugacga ggacagagcc aaagucgagg uaaccccaaa cuccccgcgc     1080

gcugaagcca cccuaggagg cuucggaagc cugggccuug acugcgaacc ccgaacagga     1140

cuggacuucu cagaccugua cuacuugacc augaacaaca aacacuggcu gguccauaaa     1200

gaaugguucc acgacauccc ccuuccaugg caugcaggcg cagacaccgg cacaccccac     1260

uggaacaaca aagaagcccu cgucgaguuc aaggacgcac acgcaaaacg ucaaaccguu     1320

gucguccuag gcucacaaga aggagcaguu cacaccgccc ucgcgggcgc ccuagaggca     1380

gaaauggacg gagccaaagg gcgccucuuc agcggccacc uaaaaugccg ccuaaaaaug     1440

gacaaacucc gccugaaagg aguaucguac ucccucugca ccgcagcauu caccuucacc     1500

aaaguacccg cggagacccu acacggaaca guaaccguug agguccaaua ugccggaacc     1560

gacggcccau gcaagauccc cguacagaug gccguagaca ugcaaacacu gacaccaguc     1620

ggacgccuaa ucaccgcaaa ccccgugauc acagagucca ccgaaaacag caaaaugaug     1680

cucgagcucg acccaccuuu cggcgacagc uacaucguca uaggagucgg agacaagaaa     1740

auaacccacc acuggcaccg aagcgggucc acgaucggca aagccuucga agcgacaguu     1800

agaggagcca aacguauggc cguacuuggc gacaccgccu gggacuucgg aagugucggc     1860

ggcgucuuca acucacucgg caaaggaauc caccaaaucu ucggagccgc guucaaauca     1920

cuauucggag gaauguccug guucucccaa auccuuauag gaacacuccu gguauggcua     1980

ggucuaaaca ccaaaaacgg cagcauaucc cuaaccugcc ucgcgcucgg cggugucaug     2040

auauuccucu cuaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9372
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794) mRNA Design 3 opt

<400>  9372
gggagaaagc uuaccaugac caguguaggc aucgucgggc uccuacucac cacggccaug       60

gcggcagagg ucacccgacg uggauccgca uauuacaugu accucgaccg aaacgacgcc      120

ggggaagcga uauccuuccc gaccacccuc ggaaugaaca aaugcuacau acaaaucaug      180

gaccuaggac acaugugcga cgcaacaaug agcuacgaau gcccaaugcu cgacgaaggc      240

guggaaccag acgacgucga cugcuggugc aacaccaccu ccacaugggu aguauacggc      300

acaugccauc acaagaaagg cgaagcccga cgguccagac gcgcugugac acucccaagc      360

cacagcacaa gaaaacucca aacacgcucc cagaccuggc ucgaaagccg ggaauauaca      420

aaacaccuca uacgcgucga aaacuggaua uuccgcaacc cgggguucgc acucgcggcc      480

gccgccaucg cauggcuccu uggcagcagc acuucacaaa aagugauaua ccucguaaug      540

auccuccuga ucgcgcccgc cuacuccauc cgcugcauag gcgucagcaa ccgcgacuuc      600

guggaaggaa uguccggcgg cacguggguc gacgucguac ucgaacacgg aggcugcguc      660

acaguaaugg cccaagacaa accaacgguc gacaucgaac uuguaaccac cacaguaagc      720

aacauggccg aaguccgcuc auacugcuac gaggcaagca uauccgacau ggcaucagau      780

ucacgcugcc ccacccaagg agaagccuac cuggacaaac aaucagacac acaauacgua      840

ugcaaacgga cccucguaga ccgcggcugg ggcaacggcu gcggacucuu uggcaaaggu      900

ucgcucguaa ccugcgccaa auucgccugc uccaaaaaga ugaccggaaa gucaauccaa      960

cccgagaacc ucgaauaccg aaucaugcuc ucgguccacg ggucccaaca caguggcaug     1020

aucgucaacg acacgggaca cgaaacagac gagaacagag ccaaaguaga aaucaccccc     1080

aacuccccaa gagcggaagc aacacucggc ggcuucggca gccuuggccu agacugcgaa     1140

ccacgaaccg gacuggacuu cagugaccuc uauuaccuaa caaugaacaa uaaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccgcuacccu ggcacgcggg ugccgacacc     1260

ggaaccccac acuggaauaa caaggaagcc cuagucgaau uuaaagacgc acacgccaag     1320

agacaaacag ucguaguacu aggcucacaa gaaggcgcag uacacacagc ccuagcagga     1380

gcgcucgaag ccgaaaugga cggcgcgaaa ggccgacuga gcagcggcca ccuaaaaugc     1440

cgacuaaaaa uggacaaacu acgccuuaaa ggagugucau acagccucug cacagcggca     1500

uucaccuuca ccaagauacc ugcagaaaca cuucacggca ccguaaccgu cgaaguacaa     1560

uaugccggaa cagacggccc augcaaggua cccgcgcaaa uggccgucga caugcaaacc     1620

cuaacccccg uaggacgacu aaucacagca aaccccguca ucaccgaauc gacagaaaac     1680

ucaaaaauga ugcuagaacu agauccccca uucggcgacu cauacauagu cauaggcguc     1740

ggcgaaaaga aaaucacaca ccauuggcac cgauccggaa gcacaauagg aaaagcauuc     1800

gaggcaacag uaagaggcgc caaacgcaug gcaguccucg gcgacacagc augggacuuc     1860

ggcagcguag gaggagcacu aaacucccug ggaaaaggaa uacaccaaau auucggcgcc     1920

gcauucaaaa gccucuucgg agggaugagc ugguucuccc aaauccuaau cgggacccuc     1980

cugauguggc uaggccuaaa caccaaaaac gggagcauca gccucaugug ccuagcacua     2040

ggcggcgucc uaauauuccu aucaaccgca guuagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9373
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(273-723) mRNA Design 3 opt

<400>  9373
gggagaaagc uuaccauguc acaaaaaguc auauaccugg ucaugauccu acucaucgcc       60

ccagccuaca gcauacgcug cauaggagua ucgaaccgag acuuugucga gggcaugagc      120

ggaggcacau gggucgacau cguccucgaa cacggcgggu gcgugacagu aauggcacaa      180

gacaaaccga caguggacau agagcuaguc acaaccacgg ucucgaacau ggcagaagua      240

aggagcuacu gcuacgaagc cagcauaagc gacauggcau cagacucaag gugcccaaca      300

caaggcgagg cuuaccucga caaacaaagc gacacccaau acgugugcaa acgcacccua      360

guagaccgcg guuggggcaa cggaugcggc cuguucggca aagggagccu agucacaugc      420

gcgaaauucg ccugcagcaa gaaaaugacu ggaaaaagca uccagccgga aaaucucgaa      480

uaccgcauaa ugcucagcgu gcacgggagc caacacucag gaaugaucgu gaacgacacc      540

ggacacgaaa cagacgaaaa cagagcgaaa guagaaauaa ccccaaacuc accaagagcc      600

gaagccacac uaggaggauu cggcucgcua ggccuugacu gcgaaccccg aaccgggcua      660

gacuucucgg accucuauua ccucaccaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccgcu acccuggcac gcaggcgcag acacaggcac accccacugg      780

aacaacaaag aggcccucgu agaauuuaaa gacgcccacg caaaacgcca aacagugguc      840

guacuaggga gccaagaagg ggcaguucac acggcccuag ccggagccuu agaggcggaa      900

auggacggag cgaaaggacg ccucucgagc ggccaucuaa aaugucgccu gaaaauggac      960

aaacucaggc ucaaaggcgu auccuacagc cuauguaccg ccgcguucac auucacaaaa     1020

auacccgcag aaacgcuaca cggcacagua acagucgagg uacaauacgc aggcacagac     1080

gggcccugca aaguacccgc gcaaauggcg guagacaugc aaacccuaac acccgucggc     1140

agacuaauaa ccgcaaaccc aguaaucaca gagagcacag agaacagcaa aaugaugcuc     1200

gagcucgacc caccguucgg agacucauac auagucauag gcgugggcga aaagaaaaua     1260

acgcaccacu ggcaccgcuc cggaucaacc auaggcaaag ccuucgaagc caccguacga     1320

ggcgccaaaa gaauggccgu gcucggagac acagccuggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9374
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(273-723) mRNA Design 3 opt

<400>  9374
gggagaaagc uuaccauguc ccaaaaaguc auauaccuag uaaugauacu acucauagcc       60

ccagcauaca gcauccgcug caucggcgua ucaaacaggg acuucguaga aggcaugagc      120

ggaggaacau ggguagacgu aguccuagaa cacggcggau gcgugaccgu aauggcacaa      180

gacaaaccga ccguagacau agagcuaguc accacaaccg ucuccaacau ggcagaaguc      240

agaagcuacu gcuacgaagc aagcaucagc gacauggcca gcgacagccg gugcccgacc      300

caaggggaag ccuaccuaga caaacagagc gacacacaau acgugugcaa acgcacacuc      360

guagaccgcg gauggggcaa cggcugcgga cuguucggca aaggaagucu agucacgugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggcaaaucga uccaacccga aaaccucgaa      480

uaccgcauca ugcuaagcgu gcacggcagu cagcacucgg guaugaucgu gaacgacaca      540

ggacacgaaa cagacgaaaa ucgcgcaaaa guggaaauaa ccccaaacuc ccccagagcc      600

gaggccaccc ucggaggcuu cggcucgcuc ggacucgacu gcgaaccccg gaccggccuc      660

gacuucuccg accuguacua ucuaacgaug aacaauaaac acuggcucgu acacaaagaa      720

ugguuccacg acauacccuu acccuggcac gccggcgcag acacaggaac gccacacugg      780

aacaacaaag aagcccucgu ggaguucaaa gacgcacacg ccaagcgcca aaccgucgua      840

guacuaggau cccaagaagg cgccguacac acggcccuag ccggagcccu ggaagcggaa      900

auggacggag caaaaggcag acucagcagc ggacaccuga aaugccgacu gaagauggac      960

aaacuaagac ucaaaggcgu gagcuacagc cucugcacag ccgcauucac guucacaaag     1020

auacccgcag aaacacuaca cggcacagua acagucgaag uccaauacgc cggcacggac     1080

gggcccugca aaguacccgc acaaauggca guggacaugc aaacgcucac acccguggga     1140

agacuaauaa cagcaaaccc agucauaaca gaaagcacag aaaacagcaa aaugaugcua     1200

gaacucgacc cacccuucgg cgacucguac auagugauag gcguaggaga aaagaaaauu     1260

acacaucacu ggcaccgcuc aggcagcaca auaggcaaag ccuucgaagc aacgguccgc     1320

ggagcaaaac gcauggccgu gcucggagac accgccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9375
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(273-719) mRNA Design 3 opt

<400>  9375
gggagaaagc uuaccauguc gcaaaaagua aucuaccuag ucaugauccu acuaaucgcc       60

cccgccuacu ccauacgaug caucggagua agcaacagag acuucgucga aggaaugagc      120

ggcggaaccu gggucgacgu aguccuggaa cauggcggcu gcgucaccgu cauggcccaa      180

gacaaaccca ccguagacau agaacucguc acaacaacag uauccaacau ggcugaaguc      240

cgaagcuacu gcuacgaagc cucaauaagc gacauggccu cagacagcag augccccacc      300

caaggagaag cauaccuaga caaacaaagc gauacacaau acguauguaa acgcacacug      360

gucgaccgcg gauggggaaa cggcugcggc cucuucggca aaggcagucu cguaaccugc      420

gccaaauuca caugcagcaa gaaaaugacc ggaaaaucca uacaaccuga aaaccucgaa      480

uauagaauca ugcuaucggu ccacggaagc caacauucgg gcaugaucgg cuacgaaacc      540

gacgaagacc gcgccaaagu agaagucaca ccgaacaguc cccgagcaga agccacccua      600

ggcggcuucg gaucccucgg ccuagacugc gaacccagaa ccggauuaga cuucagcgac      660

uuauacuacc ucaccaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac      720

auaccccuac cauggcacgc cggcgcagac accggcaccc cgcacuggaa caacaaagaa      780

gcccuagucg aguucaaaga cgcacacgcc aaacgccaaa ccguagucgu acucggaagc      840

caagaaggcg caguacacac cgcccuggcc ggagcccucg aagccgaaau ggacggcgcc      900

aaagggagac ucuucagcgg acaccuaaaa ugcagacuca aaauggacaa acuccgccua      960

aaaggcgugu ccuacucccu augcacagca gccuucacgu ucacaaaagu gccagccgaa     1020

acacuacacg gcacaguaac cgucgaaguc caauacgcgg gaacagacgg cccaugcaaa     1080

aucccagucc aaauggcagu cgacaugcaa acacucaccc ccgucggacg acuaaucacc     1140

gccaacccag ucaucaccga auccacagaa aacucaaaaa ugaugcucga acucgaccca     1200

cccuucggag acuccuacau cgucauagga gucggcgaca aaaagaucac ccaccacugg     1260

cacagaagcg gcagcaccau aggcaaagcc uucgaagcaa caguacgcgg agcaaaacgc     1320

auggccgucc uaggcgacac cgccugggac uucggguccu gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  9376
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(273-723) mRNA Design 3 opt

<400>  9376
gggagaaagc uuaccaugag ccaaaaaguc auauaccucg uaaugauccu acucauagcc       60

ccggccuaca gcauccgcug caucggagua ucaaacagag acuucguaga aggcaugagc      120

gggggaacau ggguagacgu aguccucgaa cacggaggau gcguaacagu aauggcacaa      180

gacaaaccga ccguagacau agaauuaguc acaaccacag ucucaaacau ggccgaaguc      240

cgcagcuacu gcuacgaagc gagcauaagc gacauggcaa gcgauucaag augccccacc      300

cagggggaag ccuaccuaga caaacaaagc gacacccagu acguaugcaa acgaacccuc      360

guggaccgcg gauggggaaa cgguugcggc cuauucggga aagggagccu agucacaugc      420

gcaaaauucg caugcagcaa gaaaaugacc gggaaaagca uacaaccaga aaaccuagaa      480

uaccgcauca ugcugagcgu acacgggagc caacacucag gaaugaucgu caacgacacc      540

ggacacgaaa cagacgaaaa ccgagccaaa guagaaauaa ccccuaacuc accacgagcc      600

gaagcgacac ugggcggauu cggcucgcuc ggacuugacu gcgaaccccg gaccggacua      660

gacuucucgg aucuauacua ccucacgaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acauaccccu acccuggcac gcgggcgccg acacaggaac accccacugg      780

aacaacaaag aagcacucgu agaguucaaa gacgcacacg ccaaacgcca aaccguaguc      840

guacuaggaa gccaagaagg cgccguccac acggcacuag ccggagccuu agaagccgaa      900

auggacggcg ccaaaggccg gcuaucaagu gggcaccuga aaugcagacu gaaaauggac      960

aaacucaggc ucaaaggagu gagcuacagc cugugcacgg cagcauucac auucacaaaa     1020

auaccagcgg aaacacucca cgggaccguc acggucgaag uccaauacgc aggcacagac     1080

ggacccugca aaguacccgc gcaaauggcc gucgacaugc aaacgcucac accaguaggc     1140

aggcucauaa cagcaaaccc aguaauuacc gaaagcacag aaaacagcaa aaugaugcua     1200

gaacucgacc caccauucgg agacucauac auagucaucg gcguaggaga gaagaaaaua     1260

acacaccacu ggcaccgauc aggcuccaca aucggcaaag ccuucgaggc aaccgucaga     1320

ggagccaaac gcauggcagu gcucggagac acggccuggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9377
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(F398S) mRNA Design
       3 opt

<400>  9377
gggagaaagc uuaccaugac aagcguaggc auagucggcu uacugcucac gaccgccaug       60

gcagcggaag uaaccagacg aggaagcgca uacuauaugu aucuggaccg aaacgacgcc      120

ggcgaagcaa ucagcuuccc cacaacgcuc ggcaugaaca aaugcuacau acagaucaug      180

gaccucgggc acaugugcga cgccacgaug aguuacgagu gccccaugcu cgacgaggga      240

gucgaaccag acgacgucga cugcuggugc aacaccacca gcaccugggu gguauacggc      300

acaugccacc acaagaaagg agaagcacgc cgcucucgac gagccguaac acuccccucc      360

cacagcacca gaaaacucca gacaagauca caaacauggc uagaaucacg cgaauacaca      420

aaacaccuca uaagggucga aaacuggaua uucagaaauc ccggauucgc ccucgccgcc      480

gccgcgaucg caugguuacu cggcuccucc accagccaaa aagucauaua ccuagugaug      540

auacuccuca ucgcuccagc auacuccauc cggugcauag gaguauccaa ccgagauuuu      600

guggagggaa ugucgggagg caccuggguc gacaucgucc ucgagcacgg aggcugcgua      660

acagucaugg cacaagacaa acccacaguc gacauagagc uaguuaccac aaccguaucc      720

aacauggccg aaguaagauc guacuguuac gaagcaagca ucagcgacau ggccagcgac      780

agccgcugcc cuacccaagg cgaagccuac cucgacaaac aguccgacac acaauacgua      840

uguaaacgca cccuagucga cagagguugg gguaacggcu gcggacucag cggcaaaggc      900

agccucguga ccugcgcgaa auucgccugc ucaaagaaaa ugaccggcaa auccauacaa      960

cccgaaaacc uagaauaccg caucaugcuc ucgguccacg gaucacagca cucaggcaug     1020

auagucaaug acacaggaca cgaaaccgac gaaaaccgag cuaaagucga aaucacccca     1080

aacagcccac gcgccgaagc cacgcucgga ggcuucggau cacuagggcu agacugcgaa     1140

ccccgcacag gacuagacuu cagcgaccuc uacuaccuga caaugaacaa caaacacugg     1200

cucguucaca aagaaugguu ccacgauauc ccccuacccu ggcacgcagg agccgacaca     1260

ggaaccccac acuggaacaa caaggaagcc cucguagagu ucaaagacgc acacgccaag     1320

cgacaaaccg ucguaguauu aggcucgcaa gaaggcgcag uacacacagc ccucgccggc     1380

gcacucgaag cagaaaugga cggcgccaaa gggcgacuaa guagcggcca ucucaaaugc     1440

cgacucaaaa uggacaaacu ccgacuaaaa ggcgucucau acucccuaug caccgcagca     1500

uucaccuuca ccaaaauccc ggccgaaaca cuccacggaa ccgucaccgu cgaaguacaa     1560

uacgcaggaa cggauggccc guguaaaguu cccgcgcaaa uggccgucga caugcaaacg     1620

cuuacaccag ucggcagacu cauaacagcc aacccaguaa uaaccgaaag cacagaaaau     1680

agcaagauga ugcucgaacu agacccaccc uucggagacu ccuacauagu aaucggcgua     1740

ggagaaaaga aaaucaccca ccacuggcac cgaagcggaa gcacgaucgg caaagcauuu     1800

gaagccaccg ugcgcggcgc caagagaaug gccguacucg gcgacacagc cugggacuuc     1860

gguuccgucg gaggcgcacu aaacucacug ggcaaaggca uacaccaaau cuuuggagca     1920

gccuucaaaa gccucuucgg aggcauguca ugguucuccc agauacucau cggcacccua     1980

cucauguggc uaggccugaa caccaagaac gggucaauau cacuaaugug ccuagcccuc     2040

ggcggaguac uaauauuccu gagcaccgcc guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9378
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(F398S) mRNA Design 3
       opt

<400>  9378
gggagaaagc uuaccaugac gagcgucggc aucgucggac ugcuucucac aaccgcgaug       60

gcagccgaag uaacccgcag aggauccgca uacuacaugu accuagaccg aaacgacgcc      120

ggagaagcca ucagcuuccc aaccacccug ggaaugaaua aaugcuacau acagaucaug      180

gaccugggcc auaccugcga cgcgaccaug uccuaugagu gcccaaugcu agacgaagga      240

guagagcccg augacgucga cugcuggugc aacacaacuu cgaccugggu cguguacgga      300

accugccacc acaagaaagg cgaagcacgg cgaucaagac gagccgucac acuaccaagc      360

cacagcaccc gcaagcuaca aacacgaagc cagacauggc uagaaucccg agaguacacc      420

aaacaccuaa uccgaguaga gaacuggauc uuccgcaacc caggcuucgc acuugccgcg      480

gccgcaaucg ccuggcugcu cggcagcagc acaagucaaa aggucaucua ccuggucaug      540

auacuccuaa uagcgccggc cuacagcauc cguugcaucg gcgucuccaa ccgagacuuc      600

guugaaggca ugucaggagg cacaugggua gacguaguac ucgagcacgg aggcugcgua      660

accgugaugg cgcaggacaa accaacagua gacaucgagc uagucaccac aaccgugucg      720

aacauggcug aagucaggag cuacugcuau gaagcaucaa uaucagacau ggcaagcgac      780

uccagaugcc ccacacaagg cgaagccuac cucgacaaac aaucugacac ccaauacgug      840

ugcaaaagga cccuaguaga ccggggcugg ggcaacgggu gcggccuaag cggcaaaggc      900

agccuaguaa ccugcgccaa auucgcaugc uccaagaaaa ugacaggcaa auccauccaa      960

ccagaaaacc uagaauacag aaucauguua ucaguccacg gaagccaaca cucaggcaug     1020

aucgucaacg acaccggaca cgaaacagac gaaaaccgcg cgaaaguaga aauaacccca     1080

aacucaccca gagcagaagc cacacugggc ggcuucggcu cccucggacu ggacugcgaa     1140

ccccgaaccg ggcuagacuu cucagaccua uacuaccuca caaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacauc ccacuccccu ggcacgcagg cgccgacacc     1260

ggcacaccac acuggaauaa caaagaagcc uuaguggagu ucaaagacgc acaugccaaa     1320

agacaaaccg ucgucguacu aggcagucaa gaaggcgcag uacacacagc gcucgcgggc     1380

gcgcuggaag ccgaaaugga cggagcaaaa ggcagguuau caagcggcca ccucaaaugc     1440

cgacuaaaaa uggauaaacu acgccucaaa ggcguaagcu acagccucug cacagcagca     1500

uucaccuuca ccaaaauacc cgccgaaaca cugcacggca caguaacagu cgaaguccaa     1560

uacgcaggca ccgacggacc augcaaaguc cccgcccaga uggccgucga caugcaaacc     1620

cucacucccg uagggcgcuu aaucaccgcc aacccaguca ucacagaauc cacagaaaac     1680

aguaaaauga ugcucgaacu cgacccgccu uucggagacu ccuacaucgu aaucggcgua     1740

ggcgaaaaga agaucaccca ucacuggcac agaucaggua gcaccaucgg caaagcguuc     1800

gaagcaaccg ugcgcggcgc gaaacgcaug gcaguucucg gcgacacugc augggacuuu     1860

ggcucaguag gaggcgcacu caacagccua ggaaaaggaa uacaccaaau auucggcgcc     1920

gcauucaaaa gccuauucgg cggaauguca ugguucagcc aaauacucau uggcacccuc     1980

cucauguggc ugggccucaa cgccaaaaac ggcuccauau cgcucaugug ccucgcacua     2040

ggcggcguau uaauauuccu cucaacagcu gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9379
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-790)(F398S) mRNA Design 3 
       opt

<400>  9379
gggagaaagc uuaccaugac cuccauaggc auaaucggcc uacugcuuac gacagcaaug       60

gccgcagaaa uaacacgacg gggcuccgcc uacuacaugu accucgaccg cucggacgcc      120

ggaaaagcaa ucaguuucgc uacaacccuc ggcguaaaca aaugccacgu ccaaaucaug      180

gacuuaggcc acaugugcga cgcgacuaug uccuacgaau gccccauguu agacgagggc      240

gucgaacccg acgacgucga cugcuggugc aacaccaccu cgacaugggu agucuacggc      300

acuugccauc acaagaaagg agaagcacgc cguucaagac gagcagucac ccuacccagc      360

cacagcacac gaaaacuaca aacccgcucc caaaccuggc uagaaagccg cgaauacacc      420

aaacaccuca uaaaagucga aaacuggaua uuccgaaacc ccggcuucgc acucgucgcc      480

guagccaucg cauggcuccu aggauccagc accagccaga aagucauaua ccuagucaug      540

auucuacuca ucgcaccggc auacuccaua cgcugcaucg gaguaagcaa cagagacuuc      600

gucgaaggua uguccggcgg cacuuggguc gacgucgucc ucgaacacgg aggaugcgug      660

acagucaugg cacaagacaa accaacagug gacaucgaac ucguaaccac caccgucagc      720

aacauggccg aaguccgaag cuacugcuac gaagccagca ucagcgacau ggcuucagac      780

agccgcugcc caacgcaagg ggaagccuac cucgacaaac aaucagacac ccaauacgua      840

ugcaaacgca cccuggucga ucgcggaugg ggcaacgggu gcggccuuuc uggcaagggg      900

ucccuaguaa ccugcgcaaa auucaccugu ucaaagaaaa ugaccggcaa auccauccaa      960

ccugaaaacc ucgaauaccg uauaaugcuc aguguccacg gaucccaaca cagcggaaug     1020

aucggcuacg aaacagacga agaccgcgcc aaaguagaag uaacccccaa cucgccacga     1080

gccgaggcca cacuaggcgg uuucggcucc cuaggacucg acugcgaacc ucgcacaggg     1140

cucgacuucu ccgaccuaua cuaccugaca augaacaaca aacacugguu aguccauaag     1200

gaaugguucc augacauacc ccuccccugg cacgcuggcg ccgacacagg cacaccgcac     1260

uggaacaaca aagaagcucu aguagaauuu aaagacgccc acgccaaacg acaaacaguc     1320

guuguccuag gcucccaaga gggagcagua cacacagccc uagcgggcgc ccuagaagcc     1380

gaaauggacg gcgccaaagg ccgccucuuc uccggccacc ucaaaugucg ccucaagaug     1440

gacaaacucc gccuaaaagg cguaucuuau agccuaugca cagcagcauu cacauucaca     1500

aaaguaccag ccgaaacgcu ccacggaacg gucacagucg aaguccaaua cgccggcacc     1560

gacggaccgu gcaagauccc aguacaaaug gcaguagaca ugcagacccu aacaccaguc     1620

ggccgccuga uaacugccaa cccagucaua accgaaucga ccgaaaacuc aaagaugaug     1680

cuugagcuug acccacccuu cggcgacucc uacauuguaa ucggcgucgg ggacaagaaa     1740

aucacccacc acuggcacag aagcggaucc accauaggaa aagccuucga agcaaccgua     1800

agaggagcca aaagaauggc aguccucggc gauacagcuu gggacuucgg aagcgucgga     1860

ggcgucuuca acucccucgg uaaaggcauc caccaaauau ucggcgcagc auucaagucc     1920

cuguucggcg gaaugucaug guucucacag auacuaaucg gaacacugcu aguauggcua     1980

ggccucaaca ccaaaaacgg cagcaucagc cuuacgugcc uagcacuggg cggagucaug     2040

aucuuucucu ccaccgccgu auccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9380
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(F399S) mRNA Design 3 
       opt

<400>  9380
gggagaaagc uuaccaugac aucaguaggg auaguaggac uccuccugac aacagccaug       60

gccgccgaag ucacaagacg uggcagcgcg uacuacaugu accuggauag aaacgacgca      120

ggcgaagcaa ucaguuuccc cacaacacug ggcaugaaca agugcuacau ccaaaucaug      180

gaccugggcc acaugugcga ugccaccaug agcuacgaau gccccaugcu agacgaaggc      240

gucgaacccg acgacgucga cugcuggugc aacacaacaa guacgugggu cguguacggc      300

acaugucacc acaaaaaggg agaagcucgg cggucccgcc gagccgucac gcuccccagc      360

cacagcacaa gaaaacucca aacacgaagc caaacauggc uagaaucaag agaauacacc      420

aaacaccuca uaagagugga aaacuggauc uucagaaacc caggcuucgc acuagccgca      480

gccgcaaucg cgugguuacu uggcuccagc acuucucaga aagucauaua ccugguaaug      540

auacuacuaa uagccccggc auacucgaua cgaugcaucg ggguaaguaa cagggacuuc      600

gucgaaggaa uguccggagg cacaugggua gacguagucc uagaacacgg cggaugcguu      660

acgguaaugg cccaagacaa accaaccgua gacaucgaac uggucacaac aaccguaagc      720

aacauggcag aggucagaag cuacugcuac gaagccucaa uaagcgacau ggcaagcgau      780

ucccggugcc caacucaagg ggaagcauac cuagacaaac aauccgacac acaauacgua      840

ugcaaacgca cacuuguaga cagaggcugg gggaacggcu gcggacuauc cggaaaaggc      900

ucguuaguga caugugcaaa auucgcaugc agcaagaaaa ugacagggaa aucaauacaa      960

cccgaaaacc uagaauaucg gaucaugcuc uccguacacg gcucacaaca cagcggaaug     1020

aucguaaacg acaccggaca cgaaacagau gaaaaccgag cuaaaguaga aaucaccccc     1080

aacagcccaa gagcagaagc aacacucgga ggauucgguu cacuuggacu cgacugcgag     1140

cccagaaccg gccucgacuu cucagaccua uacuaccuaa cgaugaauaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccccugccgu ggcacgcagg ggcggacaca     1260

ggaaccccac acuggaacaa caaagaagcg cuggucgagu ucaaagacgc ccacgcuaaa     1320

cgccaaaccg uaguaguacu cggaagccag gaaggagccg uacacaccgc ccuggccggg     1380

gcacucgaag cagaaaugga cggcgccaaa ggccgccuaa gcagcggaca ccuaaaaugc     1440

cgucugaaaa uggacaaauu gcgacucaag ggaguauccu acucccuuug caccgcagca     1500

uucacauuca caaaaauacc cgccgaaaca cuacauggca caguaacagu agagguccaa     1560

uacgcaggca cggacggucc augcaaagua cccgcgcaaa uggccgucga caugcaaaca     1620

cugacuccag ucggcagacu cauaaccgca aaccccguaa uaaccgaaag uaccgaaaac     1680

agcaaaauga uguuagagcu agacccaccc uucggcgaca gcuacauagu aauaggaguc     1740

ggcgagaaaa agauaaccca ccacuggcac cgcagcggaa gcaccaucgg caaagccuuc     1800

gaagccacag uccgcggggc caaacgaaug gcaguccugg gcgacacagc augggacuuc     1860

gggucagucg gcggcgcacu aaacagccuc gggaaaggca uacaccaaau cuucggugca     1920

gcuuucaaaa gccuauucgg ugggauguca ugguucagcc aaauacucau aggcacccuc     1980

cucauguggc ugggccucaa caccaaaaac ggcucaauaa gccuaaugug ccuagcacua     2040

ggcggcgucc ugauauuccu guccacagcc guauccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9381
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794)(N444Q) mRNA Design
       3 opt

<400>  9381
gggagaaagc uuaccaugac cagcgucgga auagucggac uccuccucac aacagcaaug       60

gccgcggaag ucacccgacg cggcagcgcu uacuacaugu accucgaccg aaacgacgcc      120

ggcgaagcca ucagcuuccc caccacccug ggcaugaaca aaugcuacau acaaauaaug      180

gaccuuggcc acaugugcga cgcgaccaug agcuacgaau gccccaugcu cgacgaagga      240

guggagccag acgauguaga cuguuggugc aacacaacau caaccugggu cgucuacggc      300

acaugccacc acaagaaggg agaagccaga cgcagccgca gagcaguaac ccuaccgucc      360

cacucuacca gaaaacucca aacacgaagc cagacauggu uagaaagccg cgaauacaca      420

aaacaccuca ucagagucga gaacuggaua uucagaaacc caggcuucgc auuagccgca      480

gccgcaaucg ccuggcuccu agggagcuca accucacaaa aaguaauaua ccuagucaug      540

auacuacuca uagccccggc cuacagcaua cgaugcauag gugucucaaa cagggacuuc      600

gucgaaggca ugagcggagg cacgugggug gacauaguac ucgaacacgg cggcugcgug      660

accgucaugg cacaagacaa accgacagua gacauagaau uggugacaac cacagucucc      720

aacauggccg aagugcguuc auacugcuac gaagcaagca ucagcgacau ggccucagac      780

agccgaugcc caacccaagg cgaagccuac cuagacaagc aaagcgacac acaauacguc      840

ugcaagcgga cgcuagugga ccgaggcugg ggcaacggcu gcggacuauu cggcaaagga      900

agccucguaa caugcgcgaa auucgcgugc agcaagaaaa ugaccggcaa auccauccaa      960

cccgaaaacc ucgaauacag aauuaugcuc uccguacacg gcagccaaca cuccggcaug     1020

auuguacaag auacaggcca cgagacagac gaaaacagag cgaaagucga aauaaccccc     1080

aacucgcccc gggccgaagc aacucuagga ggauucggau cgcuagggcu ugacugcgaa     1140

ccccgaacag gacuagacuu cuccgaccug uacuaccuaa caaugaacaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacauc cccuuacccu ggcacgcagg cgccgacaca     1260

ggaaccccac acuggaacaa caaagaagcc uuaguggagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg uaguaguacu aggaagccaa gaaggagcag uacacacagc cuuggccgga     1380

gcguuagaag ccgaaaugga cggggcaaaa ggcaggcuua gcucgggaca cuuaaaaugc     1440

agacuaaaaa uggacaagcu ccgccugaaa ggaguauccu acucccuaug cacagccgcg     1500

uucacauuca cgaaaauacc cgccgaaacu uuacacggca cggucacagu cgagguccaa     1560

uacgcaggca ccgacgggcc augcaaagua cccgcccaaa uggccgucga uaugcaaacc     1620

cuaaccccag uaggaaggcu uauuaccgcc aacccaguca uaacagaaag cacagaaaac     1680

ucaaaaauga ugcuagaacu agauccaccc uucggagacu ccuacaucgu cauaggcguu     1740

ggagaaaaga aaaucacaca ccacuggcac agaucaggaa gcaccauagg caaagccuuu     1800

gaagcgacag uacgcggcgc aaagagaaug gccguacuug gcgacacagc cugggacuuc     1860

ggcucagucg ggggcgcgcu gaacagccua ggcaaaggga uacaccaaau auucggagca     1920

gcauucaaau cccucuucgg cggaauguca ugguucucac aaauccucau cggaacccua     1980

cugauguggc uaggccuaaa caccaaaaac ggcagcaucu cacucaugug ccuggcccua     2040

ggcggagucc ucaucuuccu cuccaccgcc guaucggccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9382
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) Polyprotein(108-794)(N444Q) mRNA Design 3
       opt

<400>  9382
gggagaaagc uuaccaugac cagcguagga aucgucgggc uacuacucac aacagcaaug       60

gcggccgagg ucacccgcag aggauccgcc uacuacaugu accuagaccg aaacgacgca      120

ggcgaagcga ucagcuuccc aacuacacua ggaaugaaca aaugcuacau acaaauaaug      180

gaccuuggac acacaugcga cgccaccaug agcuacgaau gcccaaugcu ggacgaggga      240

guugagcccg acgaugugga cugcuggugc aacaccacga gcacgugggu ugucuauggc      300

acaugccacc acaagaaagg cgaagccaga cgcagccgac gcgcaguaac acucccauca      360

cacucaacaa gaaaacuuca aacaagaagc caaaccuggc uggaaagccg agaauacaca      420

aaacaccuga uacgagucga aaacuggauc uuccggaacc ccggauucgc acucgcggcu      480

gcagccaucg ccuggcuacu cggcuccagc acaucccaaa aagucaucua ccucgugaug      540

auauuauuga uugcacccgc auacucaauc agaugcaucg gcguaagcaa ucgugauuuc      600

guggagggga ugucaggcgg caccugggug gacgucguac uagaacacgg gggaugcguc      660

acaguuaugg cccaagacaa accaacaguc gacaucgaac ugguaaccac aaccguaagc      720

aauauggccg aggugcgauc auacuguuac gaggccagca uaagcgacau ggcuucagac      780

ucccgcugcc ccacccaagg agaagcguac cuagacaaac aaucagacac acaauacgua      840

ugcaaaagga cccuagucga ccgaggaugg ggcaacggau gcgggcuauu cggcaagggc      900

agccuaguaa cuugcgccaa auucgcaugc uccaagaaaa ugacaggcaa aagcauacaa      960

ccagaaaacc uagaauacag aaucaugcua ucgguacacg gaagccaaca cagcgggaug     1020

auaguacaag acacgggaca cgaaacagac gaaaacaggg ccaaaguaga gauaacgccc     1080

aacagucccc gggcagaagc aacacuaggc ggcuucggau cacuaggccu agacugcgaa     1140

ccacgcacag gccuagacuu cagcgaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cugguacaua aagaaugguu ccacgacaua ccguuaccgu ggcacgcagg cgcagacacc     1260

ggcacacccc acuggaacaa caaagaggcc cucguagagu uuaaagacgc ccacgcaaaa     1320

cggcaaacag ucguaguccu aggaucgcaa gagggcgcag uccacacagc acuagcaggc     1380

gcccuagaag ccgaaaugga cggagccaaa ggacgacucu ccuccggcca ccuaaaaugc     1440

agacucaaga uggauaaacu ccgacuaaaa ggcgucuccu acucgcucug cacagcggca     1500

uuuacauuca ccaaaauacc agccgaaacc uuacacggaa ccguuaccgu cgaaguacag     1560

uacgcaggaa cagacgggcc augcaaaguc ccggcacaga uggccgucga caugcaaacc     1620

cucaccccag ucggccgacu caucacggcc aaccccguga ucacagaauc gaccgaaaau     1680

ucaaaaauga ugcuagaacu ugacccaccc uucggagaca gcuacaucgu cauaggcguc     1740

ggcgagaaga aaaucaccca ucacuggcac cgcagcggaa gcaccauagg aaaggccuuc     1800

gaggcaaccg ugagaggagc gaaacgaaug gccguacucg gcgacacugc augggacuuc     1860

ggcuccgucg gcggagcucu aaacucccug ggaaaaggaa uccaccaaau auucggagcc     1920

gccuucaaaa gccuauucgg aggaaugucc ugguucucac aaauacuaau aggcacccuc     1980

cugauguggc uaggacuaaa cgcaaagaac ggguccauaa gccugaugug ccuggcgcuc     2040

gggggcgucc uaaucuuccu auccacagca guauccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9383
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) Polyprotein(108-794)(N444Q) mRNA Design 3 
       opt

<400>  9383
gggagaaagc uuaccaugac aagcguagga aucgucgggc uccugcuaac gacagcaaug       60

gcagcagaag ucacucgacg aggcuccgca uacuacaugu accuggaccg aaacgacgcc      120

ggagaagcca uauccuuccc aaccacacuu ggcaugaaca aaugcuauau ccaaauaaug      180

gaccucggac acaugugcga ugccacaaug ucauacgaau gcccaaugcu cgacgaaggc      240

guggaaccag augacgucga cugcuggugc aacaccacaa gcaccugggu cguauacggu      300

acaugccacc acaagaaagg cgaagcacgg aggagccgac gagcaguaac ccuaccaagc      360

cacuccacuc gcaaacucca aacccgaagc caaaccuggc uagaaagccg agaauacacc      420

aaacaccuaa uacggguaga aaacuggauc uuccgaaauc ccggcuucgc acuagccgcc      480

gcggcaaucg cauggcucuu aggcucaagu acaagccaaa aaguaaucua ccucgucaug      540

auucuacuaa ucgcccccgc cuacuccaua cgcugcaucg gaguaagcaa cagagacuuc      600

gucgaaggga ugucaggcgg uaccugggug gacgucguac uagaacacgg cggcugcgua      660

accgucaugg cccaagacaa accaacggua gacaucgaac ucgucaccac cacagucagc      720

aacauggccg aagugcgcuc cuauugcuau gaagcaucaa uaucagacau ggcuucagac      780

ucacgaugcc caacacaagg agaagccuac cuagacaaac agucagacac ccaauacgua      840

ugcaaacgaa ccuuagucga ucgcggaugg ggaaacggcu gcggccucuu cgggaaaggc      900

ucgcuaguga caugcgccaa auucgcaugc uccaagaaaa ugaccggcaa aucaauccaa      960

ccagagaacc uagaguauag gauaaugcuc ucaguccacg gaucgcaaca uagcggaaug     1020

auaguccaag acacgggaca cgagacggac gagaaccgcg caaaagucga aauaacacca     1080

aacagccccc gagcggaagc gacacuaggc ggguucggaa gccuaggacu agacugcgag     1140

ccacgaacgg gccuggacuu cucagaccua uacuaccuca ccaugaacaa caaacauugg     1200

cuaguccaca aagaaugguu ccacgacauc ccccucccau ggcacgcggg ugcagacaca     1260

ggcacucccc acuggaacaa caaagaggcc cuggucgaau uuaaagacgc ucacgcgaaa     1320

aggcaaaccg ucguaguacu gggcucacaa gaaggcgcgg uccauacagc ccucgcaggc     1380

gcauuagaag ccgaaaugga cggagcaaaa ggacgccuau ccucaggcca ccucaaaugc     1440

cgacucaaaa uggacaaacu acggcugaaa ggaguauccu acagccucug cacagccgca     1500

uucaccuuca ccaaaauccc cgccgaaacc cuacacggga ccguaacagu agaaguacaa     1560

uacgcaggga cagauggccc augcaaaguc ccggcccaga uggccgucga caugcaaaca     1620

cuaacaccgg uuggacgccu caucacagcc aaccccguca uuacagaauc aacagagaac     1680

uccaaaauga ugcucgaacu agacccaccc uucggagauu cauacaucgu cauaggcguc     1740

ggagaaaaga aaauaacuca ccacuggcac cgcagcggca gcaccaucgg caaagcauuc     1800

gaagccacag uccgaggagc aaaaaggaug gcaguacucg gagacaccgc augggacuuu     1860

gguagcgucg gcggcgcccu aaacucacua ggaaaaggaa uacaucaaau cuucggagca     1920

gcauuuaaaa gccucuucgg uggaaugagc ugguucuccc aaauacuaau cggaacccuc     1980

cugauguggc uaggacugaa caccaaaaac ggaagcauca gccugaugug ccucgcgcuc     2040

gggggcguac uaaucuucuu aagcacagca guaucggccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9384
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  9384
gggagaaagc uuaccaugac aagcguugga aucgucgggc ugcuacucac gaccgccaug       60

gccgcugaag ucacgcgcag gggcucagca uacuacaugu accucgacag aaacgacgcg      120

ggcgaagcaa ucagcuuccc aacaacacuc ggcaugaaca aaugcuacau acaaauaaug      180

gaccucggac acaugugcga cgcaacaaug ucguacgaau gccccaugcu cgacgaagga      240

guugaacccg acgacguaga cugcuggugc aacacaacaa guaccugggu cguguacgga      300

accugccacc acaagaaagg agaagcuagg cggucacgac gcgcaguaac ccugccaagc      360

cacuccacga gaaaacuaca aacccgcagc caaacauggc uggaaucacg cgaauacacc      420

aaacaccuca uccgcgugga aaacuggauu uucagaaacc caggcuucgc gcuagccgcc      480

gccgccauag cauggcuacu cgggucaucc acaagccaaa aagucauaua ccucgucaug      540

auccuguuaa ucgccccagc guacagcaua cggugcaucg gcgucagcaa ucgagacuuc      600

guggaaggca uguccggcgg aaccuggguc gacaucgucc ucgagcacgg agggugcgua      660

accgucaugg cacaagacaa accaacagua gacauagaac ucgugacaac aacaguaucu      720

aauauggccg aaguccgcuc cuacugcuac gaagccagca ucucagacau ggccuccgac      780

ucacgcugcc caacgcaagg ggaagcauac cucgacaaac aauccgacac ccaauacgua      840

ugcaaaagaa cacucguaga ccgagggugg ggaaacggau gcggccuauu cggaaaaggc      900

agccucguaa ccugcgccaa auucgcgugc uccaagaaaa ugacgggcaa aagcauccaa      960

cccgaaaacc ucgaauacag aaucaugcug uccguccacg gcucccaaca cuccgggaug     1020

aucguaaacg acacgggaca cgaaaccgac gaaaaccgag ccaaaguaga aaucacccca     1080

aacaguccca gagccgaagc caccuugggu gguuucggaa gccucggccu cgacugcgaa     1140

ccgagaacag gccuggacuu cagcgaccuc uacuaccuca caaugaacaa caaacacugg     1200

uuaguccaua aagaaugguu ccacgacaua ccucuccccu ggcacgccgg cgcagacacc     1260

ggaaccccac acuggaacaa caaagaggca cuuguagagu ucaaagacgc ccacgccaaa     1320

cgacaaaccg ucguaguccu cggaucacaa gaaggcgcgg uacacaccgc acucgcgggg     1380

gcccuggaag cagaaaugga cggcgccaag ggcagacuca gcuccgggca ccucaaaugc     1440

agacugaaaa uggacaagcu ccgacucaag ggagucucuu acucacuaug caccgccgcg     1500

uucacauuca cuaaaauccc agccgaaaca cuccacggua ccgucaccgu agaaguccag     1560

uaugcaggca cugacggccc gugcaaagug cccgcccaaa uggccguaga caugcaaaca     1620

cucaccccag uaggaaggcu aaucacugca aaccccguga uaacagaauc aaccgaaaac     1680

ucaaaaauga ugcuagaacu agacccaccc uucggagacu ccuacaucgu cauaggagug     1740

ggggaaaaga aaaucacaca ccacuggcac agguccggcu ccacacucgg caaagccuuc     1800

uccaccaccc uaaaaggcgc acaacgccua gcagcccuag gagacacggc gugggacuuc     1860

ggguccauag gcggcguauu caacuccauc ggacgcgcag uccaccaagu cuucggcgga     1920

gccuuccgaa cccuauucgg cggaaugagc uggauaaccc agggacugau gggagcacua     1980

cugcuaugga ugggcgucaa cgcacgcgac agaagcauag cacuagccuu ccucgccacu     2040

ggcggaguac ucguauuccu ggcaacaaac guccacgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9385
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  9385
gggagaaagc uuaccaugac cagcgucgga aucgucggac uccugcuaac aacagcaaug       60

gcagccgaag ucacgcgaag gggaucggca uacuacaugu aucucgaccg caacgacgcg      120

ggcgaagcca ucagcuuccc aacaacgcug ggcaugaaca aaugcuacau acaaauuaug      180

gaccuaggac acacaugcga cgcaacaaug agcuacgaau gccccaugcu cgacgagggc      240

guugaacccg acgacgucga cugcuggugu aauacgacau ccacaugggu aguauacggg      300

accugccacc acaagaaagg ggaagcgcgc cgguccagga gggccgucac ucucccgagc      360

cacaguacca ggaaacuaca aacccgcagc caaaccuggc ucgaaagccg ugaauauaca      420

aaacaccuaa uucgcguaga gaacuggauc uuccgcaacc ccggauucgc ccuggcagca      480

gcagcaauag ccuggcuguu gggcucaagc acaagccaaa aggucauaua ccugguaaug      540

auccuacuaa uagcaccagc auacuccauc agaugcauag gcgugagcaa ccgagacuuc      600

guugaaggaa ugucgggcgg gacauggguc gacguagucc uagaacacgg aggcugcgua      660

acggucaugg cucaagacaa gcccacgguc gacauagagc uaguuacuac aacaguaagc      720

aacauggccg aagucaggag cuauugcuac gaagccucca ucucagacau ggcauccgac      780

ucccggugcc ccacccaagg agaagcauac cucgacaaac aaagcgacac gcaauacguc      840

ugcaaaagga cccucgucga ccgcggaugg ggcaacggau gcgggcuauu cggaaaagga      900

ucccucguga ccugcgccaa auucgccugc uccaagaaaa ugacaggcaa aagcauacaa      960

cccgagaauc uggaauacag aauuaugcuc agcguacacg gcucacagca cagcggcaug     1020

aucguaaacg acaccggaca cgaaacggac gaaaaccgag caaaagucga aauaacacca     1080

aacagcccuc gcgcggaagc gacccuggga ggauucggga gccuaggacu ggacugcgaa     1140

ccccgcacgg gccuagacuu cagcgaucuc uauuaccuaa ccaugaacaa caaacacugg     1200

cuagugcaca aagagugguu ccacgacauc ccacucccgu ggcacgccgg agccgacaca     1260

ggaaccccgc acuggaacaa caaagaggcc cuuguagagu ucaaagacgc acaugcgaaa     1320

agacaaacag ucguaguccu gggcucacaa gaaggagccg uacacacagc acuagcaggc     1380

gcucucgaag ccgaaaugga cggagccaaa gggcgccuaa gcuccggcca ccugaaaugc     1440

cgccuaaaaa uggacaaacu acgccucaag ggaguaucau auucccuaug caccgcagcc     1500

uucacauuca caaaaauccc agccgaaaca cuacacggca caguaacagu cgaaguacaa     1560

uacgcaggaa cagacggccc augcaaaguc cccgcccaaa uggcgguuga caugcaaacc     1620

cuaaccccag ucggacgccu gaucacagca aacccaguca ucaccgaauc cacagagaac     1680

ucaaaaauga ugcucgaacu agacccgccc uucggcgaua gcuacaucgu aauaggggua     1740

ggagaaaaga aaaucacaca ucacuggcac cgcagcggaa gcacccuagg caaagcauuc     1800

ucgaccacac ugaaaggagc acaacggcuc gcagcccucg gagacaccgc cugggacuuc     1860

ggauccauag ggggagucuu caacucgauc ggccgcgcag uacaucaagu auucggcggc     1920

gcguucagga cacuauucgg aggaaugagc uggaucacac aaggccuaau gggagcccua     1980

cuacucugga ugggcgucaa cgcacgagac cggagcauag cccuagcauu ccucgccacc     2040

ggaggcguac ucguauuccu agcaacgaac guacacgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9386
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) Polyprotein(108-690)-Cterm-JAEV1-E(400-500) 
       mRNA Design 3 opt

<400>  9386
gggagaaagc uuaccaugac cagcaucgga auaaucggac uccuccuaac gaccgcaaug       60

gcagcagaaa uaacacgacg aggcucagcc uacuacaugu accuggaccg cagugacgca      120

ggaaaagcga ucucguucgc aacuacccuc ggcgucaaca aaugccacgu ccaaaucaug      180

gaccucgguc acaugugcga cgcaaccaug uccuacgaau gcccaauguu agaugaaggu      240

guagaaccag acgacguaga cugcuggugc aacacgacgu caaccugggu cgucuacggu      300

accugccauc acaagaaagg cgaagcccgc cgcagcagac gcgccguaac ccuaccaagc      360

cacucaacac gaaaacucca gaccagauca cagacguggc uggaaucccg agaauauacc      420

aaacaccuaa uaaaagucga aaacuggaua uucagaaauc ccggauucgc ccucgucgca      480

guagcaaucg cuuggcuacu cggauccagc acaagccaaa aaguaaucua ucucguaaug      540

auacuccuaa uagcacccgc auacuccaua agaugcaucg gcguaagcaa ccgagacuuc      600

gucgaaggaa ugucgggcgg cacaugggua gacgugguac uagagcacgg aggaugugua      660

acggucaugg cacaagauaa accgaccguc gacauagaac ucguaacaac caccguaucc      720

aacauggccg aagucagaag cuacuguuac gaagccucca ucucagacau ggccucagac      780

ucccgcugcc ccacacaggg cgaagcauac cuagacaagc aauccgacac acaauauguc      840

ugcaaacgaa cgcuagucga ccgaggaugg gggaacggau gcggacucuu ugguaagggc      900

ucacucguaa ccugcgccaa auuuaccugc agcaagaaaa ugaccggaaa aagcauccaa      960

ccggaaaacc uagaauacag aauuaugcuc ucgguccacg gcucacaaca cagcgggaug     1020

auaggcuaug aaaccgacga agacagagcc aaaguagagg ucacaccaaa cagccccagg     1080

gcagaagcaa cccucggcgg cuucggaucc cucggccucg acugugaacc ccgaaccggc     1140

cuggacuucu ccgaccucua cuaccugacc augaacaaca agcacugguu aguucacaaa     1200

gaaugguucc augacauccc ccuacccugg cacgccggcg cagacaccgg caccccacac     1260

uggaacaaca aagaagcccu agucgaguuc aaagacgcac acgccaaacg gcaaacaguc     1320

guaguccuug gcucccaaga aggagccgua cacaccgcac ucgccggagc acuagaagca     1380

gaaauggacg gugccaaggg ccgacucuuc agcggacacc uaaaaugccg ccucaagaug     1440

gacaagcuaa gacucaaagg ggucuccuac ucucucugca cugcagccuu caccuucacg     1500

aaaguccccg cagaaacccu ccacgggacu gucaccguag aaguacaaua cgccgguacc     1560

gacggcccau gcaaaauccc aguacaaaug gcaguagaca ugcagacacu uacacccgua     1620

ggacgccuaa uaacagcaaa ccccgucaua accgaaagca cagaaaacuc caaaaugaug     1680

cuagaacuag acccucccuu cggagacagc uacauaguga ucggcgucgg cgacaagaaa     1740

aucacacacc acuggcacag aagcggcagc acccuaggca aagcauucuc uacaacccuc     1800

aaaggcgccc aacgacuagc agcacucgga gacacagccu gggacuucgg aagcaucgga     1860

ggcgucuuca acagcauagg acgcgccgua caccaagucu ucggaggcgc uuuccgcacc     1920

cucuucggcg gaaugagcug gaucacccaa gggcugaugg gcgcacuacu acucuggaug     1980

ggaguaaacg cacgggacag aagcaucgcc cuggcuuuuc ucgccaccgg cggcguccuc     2040

gucuuccuag caacgaacgu acaugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9387
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) 
       Polyprotein(108-694)-Cterm-JAEV1-E(400-500) mRNA Design 3 opt

<400>  9387
gggagaaagc uuaccaugac cuccgucggc auaguaggcc uacuacucac caccgccaug       60

gccgccgaag ucacgcgcag aggaagcgca uacuacaugu accuggaccg aaacgaugcc      120

ggcgaagcga ucuccuuccc aaccacucuc gggaugaaca aaugcuauau ccaaaucaug      180

gaccuaggac acaugugcga ugcaaccaug uccuacgagu gcccaaugcu agaugaagga      240

gucgaaccag acgacgugga cugcuggugc aacaccaccu cgacaugggu agucuacggc      300

acaugccacc auaagaaggg ggaagccaga cgcuccagac gagcaguaac gcuccccuca      360

cacucaacga gaaaacucca aaccagaagc cagacauggc ucgaaucacg cgaguacacc      420

aaacaccuca uccgcgucga aaacuggauc uuccgaaauc cuggcuucgc ccuagcagca      480

gccgccauag ccuggcuacu cggcagcagc accagccaaa aaguaaucua ucuaguaaug      540

auccuacuaa ucgcacccgc cuacagcaua cgcuguaucg guguaucaaa ccgcgacuuu      600

gucgaaggaa ugucaggcgg cacauggguc gacguagucc uagaacacgg cggcugcguc      660

acaguaaugg cacaagacaa accaacgguc gacauagagc uaguaacaac aacgguaucc      720

aacauggccg aaguccgauc auacugcuac gaagccucca ucucagacau ggcaucggac      780

ucccgcuguc cgacacaagg agaagcguac cuagacaaac aaucggacac ccaauacgua      840

ugcaaacgaa cucuaguaga ccgcggcugg ggaaacggau guggccucuu cgguaagggc      900

ucacucguga ccugcgcaaa auucgcaugc agcaagaaaa ugaccggcaa auccauccaa      960

ccugaaaacc uagaauaccg aauaaugcua ucaguccacg guucacaaca cuccggaaug     1020

aucguaaacg acaccggcca cgaaacagac gaaaaccgag ccaaaguaga aauaaccccc     1080

aacagcccac gagcggaagc aacccuaggg ggauucggcu cacuggggcu ggacugugaa     1140

cccagaacgg gccuagacuu cagcgaccuc uacuaccuaa caaugaacaa uaaacacugg     1200

cuaguacaca aagaaugguu ccacgacauc ccccuccccu ggcacgcagg cgccgauacc     1260

ggcacucccc acuggaacaa caaagaagcu cuggucgagu ucaaagaugc acacgcuaaa     1320

agacaaaccg uagucguccu aggcucccag gaaggugcgg uccacaccgc ccucgcagga     1380

gcccucgagg cagaaaugga cggcgcuaaa ggacgccuaa gcuccggcca ccucaaaugc     1440

cgccuaaaaa uggacaaguu acgccucaaa ggagugaguu acagccucug cacagcggcc     1500

uucaccuuca caaaaauccc agcagaaaca cuacacggaa ccgucaccgu cgagguucaa     1560

uacgcaggca cggacgggcc augcaaaguc ccagcccaaa uggccgucga caugcaaacu     1620

cuaaccccag uaggccgccu cauuacagcc aacccaguaa ucacugaauc caccgagaac     1680

agcaaaauga ugcucgaacu cgacccacca uucggggacu ccuacaucgu caucggcgua     1740

ggcgagaaga aaauaacaca ccacuggcac cgcucgggga gcacucucgg caaggccuuc     1800

ucaaccacac ucaaaggcgc ccaacgacuc gccgcccuag gagacaccgc augggacuuc     1860

ggcucaaucg gcggagucuu caacagcaua ggccgcgccg uacaccaagu auucggcgga     1920

gcauuccgca cacuguucgg gggaaugucc uggauaacac aaggccucau gggcgcccua     1980

uuacuuugga uggggguaaa cgcccgagac cgaagcauag cccuagccuu ccuagccacc     2040

ggagggguac ucguauuuuu agccaccaac guccacgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9388
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(93-804) mRNA Design 3 opt

<400>  9388
gggagaaagc uuaccaugcg caucauaaac gcccgcaagg aaaagaaaag acgcggggcc       60

gacacauccg ucggaauagu aggccugcua cuaacaaccg ccauggccgc cgagguaaca      120

agacgcggca gcgccuauua cauguaccuc gaccgcaaug acgcaggaga agcaauauca      180

uuccccacca ccuugggaau gaacaaaugu uacauccaaa ucauggaccu aggacacaug      240

ugcgacgcaa caaugaguua cgagugccca augcuggacg aaggagugga accagacgac      300

gucgacuguu ggugcaacac caccagcacc uggguaguau acggcacaug ccaccacaag      360

aaaggcgaag cacgacgaag ccggagagcg guaacccucc ccagccacuc cacacgcaaa      420

cugcaaacca gaucccaaac cuggcuagaa agccgcgaau acacaaaaca ucucauacgc      480

guggaaaacu ggauauuuag aaacccaggc uucgcccuag ccgccgccgc aaucgcaugg      540

cuccucggaa gcuccaccag ccaaaaaguu auuuaccugg uaaugauacu guugauagca      600

cccgccuaca gcauucggug uaucggcgua agcaacagag acuucgucga aggaaugucg      660

ggaggcaccu gggucgacau cguccuagag cacggcggcu gcguaaccgu cauggcacaa      720

gacaaaccaa cagucgacau cgagcucguc accaccaccg uaucaaacau ggccgaagug      780

cgcagcuacu gcuacgaagc aagcauauca gacauggcuu cugacagcag augccccaca      840

caaggagagg ccuaccuaga caagcaaucc gacacacagu acguaugcaa acgaacccua      900

gucgaccgag gauggggaaa cggaugcgga uuauucggca agggaucccu agucacgugc      960

gcaaaauucg ccugcagcaa gaaaaugacc ggaaaaucca uacaaccaga aaaccucgaa     1020

uacaggauaa ugcucagcgu acacggcuca caacacagug gcaugaucgu caacgacacc     1080

ggacaugaaa ccgacgaaaa ccgcgccaaa guggaaauca ccccaaacag cccacgggca     1140

gaggcaacac ucggcggcuu cggcucccua ggacuggacu gcgaacccag aaccggacuc     1200

gacuucuccg accucuacua ccuaacaaug aacaacaaac auuggcuggu gcacaaagaa     1260

ugguuccacg acauaccgcu ucccuggcac gccggcgccg acacgggaac accccacugg     1320

aacaacaaag aagcccucgu agaguucaaa gacgcacacg caaaacgaca aacggucguc     1380

guccuagggu cacaagaagg cgccguccac accgcacuag ccggcgcacu cgaagcagaa     1440

auggacggcg ccaaaggacg acucucauca ggccaccuaa aaugccgccu aaaaauggac     1500

aaacuacgcc ugaaaggcgu cagcuacagc cuauguaccg ccgcauuuac guuuaccaaa     1560

auacccgcag aaacacucca cggcacaguc acagucgaag uccaauacgc cgggaccgac     1620

ggccccugca aaguaccggc ccagauggca guggacaugc aaacgcucac cccaguugga     1680

agacugauaa ccgcaaaccc cgucaucacc gagagcaccg aaaacucaaa aaugaugcua     1740

gaacucgacc cacccuucgg cgacuccuac aucgucaucg gcgucgguga aaagaaaaua     1800

acacaucauu ggcacagauc cggaagcacc aucggaaaag cauucgaagc caccguccga     1860

ggagcaaaaa gaauggcggu ccucggcgac accgcguggg acuucggaag cgugggcggc     1920

gcccucaaca gccuagggaa agggauacac caaauuuucg gugcagccuu caaaucacua     1980

uucggcggaa ugagcugguu cucccaaaua cuaaucggua cacuacucau guggcuaggc     2040

cuaaacacca aaaacggcag cauaagccua augugucucg cucuaggcgg cguacucauc     2100

uuccucucua cagcagucag cgccgacgua ggcugcagcg ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9389
<211>  2362
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9389
gggagaaagc uuaccaugau caucaaugcu cgaaaagaga agaaacgacg uggcgcagac       60

acaucggucg gcauaguagg acucuuacuc acaaccgcaa uggcagcgga agucacccga      120

cgcgggagcg ccuacuacau guaccucgac cgcaacgaug ccggugaggc aaucucauuc      180

cccacgacac uaggaaugaa caaaugcuac auccaaauca uggaccucgg gcacaugugc      240

gaugcgacua uguccuauga gugcccaaug cuggaugaag gagucgaacc cgacgacgua      300

gacugcuggu gcaacacgac caguacaugg gucguauacg gaacgugcca ccacaagaaa      360

ggcgaagccc gccgcagccg ucgagcagug acccuaccca gccacucaac acgcaaacuc      420

caaacacguu cacaaaccug gcucgaaagc cgagaauaca cgaaacaccu aauaagaguc      480

gaaaacugga ucuuuaggaa cccgggcuuc gcacuagcag cggccgcgau agccuggcuc      540

cuggggagcu cgaccucaca aaaagucaua uaccugguaa ugauccuacu gaucgccccg      600

gcauacucua uacgcuguau aggcgucucg aaccgcgacu ucguagaagg aaugagcgga      660

gggacauggg ucgacauagu ccuagaacac gggggcugcg uaacagucau ggcccaagac      720

aaaccaaccg ucgacaucga auuagucaca acuacagucu cgaacauggc cgaaguacgg      780

agcuacugcu acgaagccag caucagcgac auggccucag acagccgaug cccaacacaa      840

ggcgaagcau accucgacaa acaaagcgac acacaguacg uaugcaaacg cacccuagua      900

gacagaggau ggggaaacgg uugcggacuc uucgggaaag gaagccuagu cacaugcgca      960

aaauucgccu gcagcaagaa aaugacuggc aaaaguauac aaccagaaaa ccucgaauac     1020

cgaaucaugc ucagcgugca cggaucacaa cacucuggga ugauaguaaa cgacaccgga     1080

caugaaaccg augaaaaccg cgccaaagua gagaucacac caaacagccc cagggccgaa     1140

gccacccucg gaggcuucgg cagccuggga cuagacugcg aaccccgcac aggccucgac     1200

uucucagauc uauacuaccu cacaaugaac aacaaacacu ggcuagucca caaagaaugg     1260

uuccacgaca ucccucuacc guggcacgcc ggcgcagaca ccggcacccc acacuggaac     1320

aacaaagaag cccuagugga guucaaagac gcacacgcaa agcgccagac aguaguagua     1380

cuagggagcc aagaaggagc aguccacaca gcacucgccg gggcccuaga agccgaaaug     1440

gacggcgcaa agggacgccu guccucggga caccuaaaau gccgacugaa aauggacaag     1500

cuccgacuaa aaggcguaag cuacagucua ugcacagccg cguucacauu caccaaaauc     1560

ccugcagaga cgcugcacgg gaccguaaca guagaaguac aauacgccgg cacggacggg     1620

cccugcaaag uaccagcaca aauggccguc gacaugcaga cacucacacc cguaggcaga     1680

cuaaucacag caaaccccgu aaucaccgag ucgacagaaa acaguaagau gaugcuggaa     1740

cucgacccac ccuucggaga cuccuacaua guaauaggcg ugggugaaaa gaaaaucaca     1800

caccacuggc accgaagcgg aagcacaaua ggcaaagccu ucgaagcaac aguaagaggg     1860

gcaaaacgca uggccguccu cggcgacaca gcgugggacu ucggaagcgu cggaggcgca     1920

cucaacuccc ucggaaaagg aauccaccaa auauucggag cagccuucaa aucccuauuc     1980

ggcgggauga guugguucag ccaaauacug auaggcaccc ugcuaaugug gcuaggacuc     2040

aacaccaaaa acgggucaau aucacucaug ugccucgcgc uaggaggagu cuuaaucuuc     2100

cuaucaacag cugucagcgc cgauguagga ugcuccguug acuuuucaaa augaggacua     2160

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2220

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2340

ucuuuucaga gccaccagaa uu                                              2362


<210>  9390
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9390
gggagaaagc uuaccaugau caacgcaagg aaggagaaga aacgccgagg ggccgacacg       60

agcgugggca uagucggccu ccuacucaca accgcgaugg cggcagaggu aacaagaaga      120

ggcagcgccu acuacaugua ccucgaccgc aacgacgcag gagaagcaau aucauuccca      180

accacccuag gcaugaacaa auguuacaua caaaucaugg accuaggcca caugugcgac      240

gccacaaugu ccuacgaaug cccaaugcua gacgagggcg uagagcccga cgacgucgac      300

ugcuggugca acaccacuag caccugggug guauauggca cuugccauca caagaaagga      360

gaagcccgcc gcuccaggag agcaguaacc cuccccagcc acagcacaag aaaacuccaa      420

acaagauccc aaacuuggcu ggaaucgaga gaauacacca aacaucuaau aagagucgag      480

aacuggaucu uccgaaaccc aggauucgca cuagccgcag cagcaauagc cuggcuucua      540

ggcucaagca caucccagaa aguaaucuac cuagucauga uacucuugau agcaccagca      600

uacucaaucc gcugcauagg aguaagcaac cgcgacuucg ucgaaggaau gagcggaggc      660

acaugggucg auaucguacu cgagcacgga ggcugcguua cggucauggc acaagacaaa      720

cccaccguag acaucgaacu gguaaccaca acagugagca acauggcaga aguacgcagc      780

uacuguuacg aagcaucaau cucagacaug gcaucagaca gccgaugccc aacacaaggc      840

gaagcguacc ucgacaaaca aagcgacaca caauacguau guaaacggac ccuggucgac      900

cgaggauggg gcaacggaug cggacuguuc ggcaaaggau cgcucguaac augcgcaaaa      960

uucgcaugca gcaagaaaau gaccggaaaa agcauacaac ccgagaaccu cgaauaccgg     1020

aucaugcucu cgguacacgg gucccaacac agcggcauga uaguaaacga cacgggacac     1080

gaaacagacg agaacagagc gaaagucgaa auaacaccaa acagcccccg agcagaagcg     1140

acccugggcg gcuucggguc ccugggacua gacugcgaac caagaacugg acuagacuuu     1200

uccgaccuau acuaccuaac caugaacaac aaacacuggc ugguacacaa agaaugguuc     1260

caugacaucc cgcucccaug gcacgccggg gccgauaccg gcacacccca cuggaacaac     1320

aaagaagccc uagucgaauu uaaagacgcc caugccaaaa gacaaacagu cgucguacug     1380

ggcagccaag agggcgccgu acacaccgcg cucgcaggcg cacuagaagc cgaaauggac     1440

ggagcaaaag gaagacuaag cucaggccac cuaaaaugca gacucaaaau ggacaaacug     1500

cgccugaaag gagugucgua cucccuaugc acagcagccu ucaccuucac aaaaauccca     1560

gcagagacgc uacacgggac agucaccgua gaggugcaau augcagggac agacggccca     1620

ugcaaaguac ccgcccaaau ggccguagac augcaaaccc uaacaccagu cggacgccuc     1680

auaacagcaa acccaguaau aacagaaucu acggagaacu cgaaaaugau gcuggaacua     1740

gauccgcccu ucggcgacag cuacaucgua auaggagucg gugaaaagaa aauaacacac     1800

cacuggcaca gauccggcag cacgauagga aaagccuucg aggcaacagu aaggggcgcc     1860

aaacgaaugg caguacuagg cgacaccgcc ugggacuucg ggucagucgg aggggcacua     1920

aacagucucg ggaaaggcau acaccaaauu uucggagccg ccuucaaaag ccuauucgga     1980

ggaaugucgu gguucucaca aauccuuaua ggcacgcuac uaauguggcu ugggcuaaau     2040

accaaaaacg gcagcaucag ccugaugugc cuagcacucg gaggcgugcu cauauuccuc     2100

agcaccgccg ucagcgcgga cgucggaugc agcguggacu ucagcaagug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  9391
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9391
gggagaaagc uuaccaugaa cgcgcgaaaa gaaaagaaaa ggcgaggggc cgacacaagc       60

guuggaaucg ucggccuacu cuugacaacg gcaauggcag cagaaguaac acggcgcgga      120

ucagcguacu acauguaccu cgacagaaac gacgccggcg aagccaucag cuuccccacg      180

acccucggca ugaacaaaug cuauauacaa aucauggacc ucggccacau gugcgacgca      240

acaaugagcu acgaaugucc aaugcucgac gaaggggucg aaccagacga cguagacugu      300

uggugcaaca caacgagcac auggguagua uacggaacau gccaccacaa gaaaggcgaa      360

gccagacgca gccgaagggc aguaacacuc cccagccacu caacacgcaa acuccaaacc      420

cgaucacaaa cguggcuaga aucccgugaa uacaccaaac accuuauacg cgucgaaaac      480

uggaucuucc gcaauccagg guucgcacuc gccgcagcag ccaucgcgug gcugcuagga      540

uccagcacau cgcaaaaagu aaucuaccua guaaugaucc uccuaauugc cccggcguac      600

ucaauccgau gcauaggagu cucaaaccgg gacuucguag aaggcaugag cggaggcaca      660

uggguagaca uuguccuaga acacggcggg ugugucaccg ucauggccca agacaagcca      720

accguggaca ucgaacucgu cacaacaacc guaucuaaca uggcagaggu aagaucauac      780

ugcuacgaag ccaguauauc cgacauggca agcgauucca gaugcccaac ccaaggcgaa      840

gccuaccuag auaaacaauc agacacccaa uacguaugca aacgcaccuu agucgaccga      900

ggcuggggca auggaugcgg ccucuucggg aaaggaagcc uagucacuug ugcgaaauuc      960

gccugcagca agaagaugac aggcaaaucc auccagcccg aaaaccucga auaccgaauc     1020

augcuaagcg uacacggcag ccaacacucu ggaaugauag ucaacgacac ugggcacgaa     1080

acagacgaaa accgcgcgaa aguggaaauc accccaaacu cacccagagc cgaagccaca     1140

cuaggaggcu ucgguucacu cggacucgac ugcgaaccaa gaaccggccu ugacuucuca     1200

gaccuauauu aucuaacaau gaacaacaag cacuggcugg ugcacaaaga augguuccac     1260

gacauaccuc ucccguggca cgcaggcgca gacacaggaa caccacacug gaacaacaaa     1320

gaagcccuag ucgaguucaa agacgcacac gccaaacgac agacuguagu cgugcuagga     1380

agccaagaag gagccgugca cacugcccuc gcaggggcac uagaagcaga aauggacgga     1440

gccaaaggaa gacuaagcag cggacaccug aaaugcagac uaaaaaugga caaacuaagg     1500

cucaaaggag ucaguuacuc acuaugcaca gccgcguuca cauucacaaa aauacccgca     1560

gaaacgcucc acggaacagu cacaguugaa guccaauacg caggcacaga cggccccugc     1620

aaagugcccg cccaaauggc aguagacaug caaacacuca cgcccguagg gagacuaaua     1680

acggcaaacc cggucauaac cgaaagcacc gaaaacagca aaaugaugcu cgaacucgau     1740

ccgccguucg gcgauagcua caucgucaua ggagucggcg aaaagaaaau cacacaccac     1800

uggcacagau ccggauccac aaucggaaaa gccuucgaag ccacagugcg aggagccaaa     1860

cguauggcgg uacucggcga cacggccugg gacuucggca gcgucggggg agcacugaac     1920

agccuaggaa agggaaucca ccaaaucuuc ggagccgccu uuaaaagucu cuucgggggc     1980

augucauggu ucagucaaau acucaucgga acacuacuca uguggcuagg ccuaaacacc     2040

aaaaacggau ccauaucacu aaugugccuu gcccuaggcg gaguccuaau auuccucuca     2100

acagcaguca gcgccgacgu cgggugcucg gucgacuucu caaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  9392
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9392
gggagaaagc uuaccauggc cagaaaagag aaaaagagac gaggagcgga cacguccguc       60

ggcaucgucg gucuccuucu gacaaccgcc auggccgcgg agguaacccg ccgcggaagc      120

gccuacuaua uguaccucga cagaaacgac gccggagaag caaucucauu ccccacaacc      180

cuaggaauga acaaaugcua cauccaaauc auggaccucg gccacaugug cgacgccaca      240

augagcuacg agugccccau gcuagacgaa ggaguagagc ccgacgacgu cgacugcugg      300

ugcaacacca ccucaaccug gguagucuac ggaacaugcc accacaagaa aggcgaggca      360

agacgcuccc gccgagccgu uacgcuuccg ucacacagca ccagaaaacu ccaaaccaga      420

ucccagaccu ggcucgaauc cagagaauac acuaaacacc uuauacgagu cgagaacugg      480

aucuucagga acccaggcuu cgcguuagca gcagccgcga ucgccuggcu acucggcagu      540

uccaccagcc aaaaaguaau uuaccucgug augauccuac uaaucgcacc ggcauauucu      600

auaagaugca uaggcgucag caacagggac uucguggaag gaaugucagg aggcaccugg      660

gucgauaucg uacuagaaca cggaggcugc gucaccguca uggcccaaga caaaccaacg      720

gucgacaucg aauuagucac cacaaccgua agcaacaugg cagaagucag aagcuacugc      780

uacgaagcau ccaucuccga cauggcaagc gacucacgcu gccccaccca aggcgaagca      840

uaccucgaua aacaaagcga cacacaauac guaugcaaaa ggacacucgu ggacagagga      900

ugggggaacg gcugcggccu auucggcaaa ggaagccucg ugaccugcgc caaauucgca      960

ugcuccaaga aaaugaccgg aaaaucaauc caaccagaaa aucucgaaua cagaaucaug     1020

cucuccgucc acggguccca acacagcggc augauaguua acgacacagg ccaugagacc     1080

gacgaaaacc gggcuaaagu agagauaacc cccaacucac cccgggccga agccacgcua     1140

gggggcuucg gaagccuagg acucgacugu gaaccgcgua cgggacucga cuucuccgac     1200

cuguauuauc ucacaaugaa caacaaacac uggcuaguac acaaagagug guuccacgac     1260

auaccgcuac cauggcacgc cggcgccgac accggaacac cacacuggaa uaacaaagaa     1320

gcccucguag aguucaaaga cgcgcacgca aaaagacaaa ccgugguagu cuuaggcuca     1380

caggaagggg caguacacac agcccuggca ggagcacuag aagccgaaau ggauggagca     1440

aaagggcgac uaagcagcgg gcaccuuaaa ugccgacuca aaauggacaa acuacgccuc     1500

aaaggagucu ccuacucgcu auguaccgcc gcauuuacau ucaccaaaau ccccgcagaa     1560

acccuccacg gaacaguaac aguagaaguc caauaugccg gcacagaugg cccaugcaaa     1620

guaccugcgc aaauggcagu cgacaugcaa acacuaacgc cugucggccg acuaaucaca     1680

gccaacccag uaaucacaga aucgaccgaa aacagcaaga ugaugcuaga acuagaccca     1740

cccuucggcg acuccuacau agucauaggg guaggagaaa agaaaaucac ucaccacugg     1800

cacaggagcg gcuccaccau aggaaaagcg uucgaagcua ccgucagagg cgcaaaacgc     1860

auggcgguac ugggagacac cgcaugggac uucggaagcg ucggaggggc ccuaaacucc     1920

cuaggaaaag gaauccacca aauauucgga gccgccuuca aaagccuauu cggaggcaug     1980

agcugguuuu cccaaauacu aaucggcacc cuccucaugu gguuaggacu aaacaccaaa     2040

aacggcagca uaucacucau gugccucgcg cuaggcggcg uccucauauu ccucagcaca     2100

gcagugucgg ccgacgucgg cugcagcgua gacuuuucaa aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  9393
<211>  2350
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9393
gggagaaagc uuaccaugag aaaagagaag aaacgaagag gagcagacac cagcguagga       60

auagucggac uccuccuaac uaccgcaaug gcagcagaag ucacgcgaag aggcagcgcg      120

uacuacaugu accuagaccg gaacgaugcc ggcgaggcca ucucauuccc aacaacacuc      180

gggaugaaca aaugcuacau acagaucaug gaccucgggc acaugugcga cgccacuaug      240

uccuacgagu gcccgaugcu ggacgaaggc gucgagccag acgacgucga cuguuggugu      300

aacacuacca gcaccugggu aguauacgga acaugccacc acaagaaagg agaagcccgc      360

aggagcagac gcgcggucac gcugccaucc cacagcacgc gcaaacucca aacuagguca      420

caaaccuggc uagagagccg agaauacacc aagcaccuaa ucagagucga aaacuggaua      480

uuccgcaacc caggauuugc acuagcggcc gccgcaauag cguggcuacu aggcucgagc      540

acaucccaaa aagugaucua ccuagucaug auacuccuaa ucgcacccgc auacagcauc      600

cgaugcauag gagucucuaa cagagauuuc gucgaaggaa ugucgggcgg aacguggguc      660

gacauaguac uagaacacgg aggcugcgua accgugaugg cccaagacaa accaaccguc      720

gacaucgagc uagucacgac uaccguaagc aacauggcag aaguacgauc cuacugcuac      780

gaagcaagca ucucagacau ggccagcgac agccgaugcc ccacccaggg cgaagcauac      840

cuagacaaac aaucagacac acaauacguc ugcaaaagaa cacucguaga ucgcggaugg      900

ggcaacggau gcggacucuu cggaaaaggc agucuaguca caugcgccaa auucgcaugc      960

ucaaagaaaa ugaccgguaa auccauacaa ccagaaaacc ucgaauaccg gauaaugcuc     1020

ucaguacaug ggagccaaca cagcggcaug auagucaacg acacagggca cgaaacugac     1080

gaaaaccgcg ccaaaguaga aauaacacca aacagccccc gcgccgaagc cacacuggga     1140

ggcuuuggaa gccucggcuu agauugcgag ccacgaacag gccuagacuu cucggaccua     1200

uacuaccuca cgaugaacaa caaacacugg cuaguacaua aagaaugguu ccaugacauc     1260

ccccucccau ggcacgcugg cgcggacacg gggacaccac acuggaacaa caaggaagcc     1320

cugguagagu ucaaggacgc ccacgcgaaa aggcaaacag ucgugguauu aggcagccag     1380

gaaggcgccg uccacaccgc auuagccggc gcccuggaag ccgaaaugga cggagcaaaa     1440

ggcagauuaa gcuccggaca ccucaaaugc cggcuaaaga uggacaaacu cagauugaaa     1500

ggaguauccu acagccuuug caccgcagcc uucaccuuca cgaaaauacc agccgaaaca     1560

cuacacggaa caguaacggu cgaagugcaa uacgcaggaa cagacggccc cugcaaagua     1620

ccagcgcaaa uggcagucga caugcaaacc cugacgccgg ucggcagacu aaucaccgca     1680

aacccaguca ucacagaauc caccgaaaac ucaaaaauga ugcuagaacu ggacccaccc     1740

uucggagacu ccuacauugu aauaggaguc ggcgaaaaga aaauaaccca ccacuggcac     1800

cgaucaggca gcacaauagg caaagcauuc gaagccaccg uaagaggcgc aaaacgcaug     1860

gcaguacugg gagacaccgc augggacuuc ggcagcguug gcggagcccu caacuccuua     1920

ggcaaaggaa uccaccaaau auucggagca gcauucaaaa gccuguuugg cgguaugagc     1980

ugguucuccc aaauacucau cgggacacug cugauguggc ucggacucaa cacgaaaaac     2040

gguagcauaa gucugaugug ccucgcccua ggcggcguac ucaucuuccu aaguacagcg     2100

guaucagcag acguaggcug cagcgucgac uucagcaaau gaggacuagu uauaagacug     2160

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2220

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2340

caccagaauu                                                            2350


<210>  9394
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9394
gggagaaagc uuaccaugaa agaaaagaaa cgccgaggcg cagacaccag uguaggcaua       60

guaggacuuc uccuaacaac agccauggcg gcggaaguaa cucgcagagg cuccgccuac      120

uacauguauc ucgaccgaaa cgacgcgggu gaagcaauca gcuucccaac cacucucggc      180

augaacaaau gcuacaucca aaucauggac cucggccaca ugugcgacgc cacaaugagc      240

uaugaaugcc ccaugcuaga cgaaggcgua gaacccgacg acguagacug cugguguaac      300

accaccucaa ccugggucgu cuacggaacg ugccaccaua agaaaggaga agcacgccgc      360

agcagacgcg cggucacauu accaagccau agcacgcgca aacuacaaac ccggucacag      420

acauggcucg agagccgaga guacaccaaa caccuaauac gcgucgaaaa cuggauauuc      480

cgaaaccccg gauucgcacu ugcagcggcc gccaucgcau ggcuccuggg cucaagcacc      540

agccaaaaag uaaucuaccu agucaugauc cugcuaaucg ccccagccua cagcaucagg      600

ugcauaggcg ugagcaaccg agacuucguc gaaggaaugu cgggcggaac cuggguagac      660

aucguacugg aacauggcgg cugcgucacc guaauggccc aagacaagcc caccgucgac      720

auagaacucg ucacaacuac cgucuccaac auggccgagg uacgcuccua cugcuacgaa      780

gccucaaucu ccgacauggc cagcgacuca cgaugcccua cgcaaggcga agccuaccug      840

gacaaacaau cggacacaca auacgugugc aaacgaacau uagucgaccg aggauggggg      900

aacggcugcg gccuguucgg caaaggcuca uuagugaccu gcgcaaaguu ugccugcagc      960

aaaaagauga ccggaaaauc cauacaacca gaaaaccuag aauacagaau caugcuguca     1020

guacauggcu cacaacacag ugguaugauc gucaacgaca cgggccacga aacagacgaa     1080

aaccgagcga aaguagaaau caccccaaac agcccgcgcg cugaagccac acucggaggu     1140

uucggcucac uaggccuaga cugcgagcca cgaacuggcc uugacuucuc cgaccuguac     1200

uaccucacca ugaauaacaa acacuggcua guacacaaag aaugguucca cgacauccca     1260

cuacccuggc acgccggcgc cgacaccggg accccacacu ggaacaacaa agaagcacug     1320

guagaguuca aagacgccca cgccaaaaga caaaccgucg uaguacuagg cagccaagaa     1380

ggugcggucc acaccgcccu agccggcgcc cuagaagccg aaauggacgg ugccaaaggc     1440

cgccuuagcu ccggucaccu aaaaugccga cuaaaaaugg acaaacugag acuaaaaggc     1500

gucucauacu cauuaugcac agcagccuuu acuuucacaa agaucccagc cgagacgcua     1560

cacggaacag uaacaguaga aguacaguac gccgggaccg acggccccug caaagucccc     1620

gcccaaaugg ccguagacau gcagacacua accccagucg ggagacugau caccgccaac     1680

ccgguaauaa cagaaagcac cgaaaacucc aaaaugaugc uugagcuaga cccaccuuuc     1740

ggggauagcu acaucgucau cgggguaggc gagaagaaaa uaacacauca cuggcauaga     1800

agcggcagca cgauaggaaa agcauucgaa gcaacagucc gcggcgcaaa acgcauggcc     1860

guuuugggcg acacagcaug ggacuucggc uccgugggcg gcgcacucaa cuccuuaggc     1920

aaaggcaucc accagaucuu cggggccgcc uucaaaucac uauucggcgg caugucaugg     1980

uucagccaga uccucauagg cacacuccua auguggcugg gacuaaauac caaaaacggc     2040

uccaucucac ucaugugccu ugcccucgga ggcguccuaa ucuuccuuag uacagccgua     2100

agcgccgacg ucggaugcuc cguagacuuc uccaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9395
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9395
gggagaaagc uuaccaugga aaagaaaaga cgcggcgccg acacgagcgu cgguauagua       60

ggacuucuac ucacgaccgc cauggcggcc gaaguaacca gacggggcag cgccuacuac      120

auguaucucg accgcaacga cgccggcgaa gccaucucgu uccccaccac acuagggaug      180

aacaaauguu acauccaaau aauggaccuc gggcacaugu gcgacgcgac aaugaguuac      240

gaaugcccaa uguuagacga aggcguagaa ccggacgacg ucgauugcug guguaacacc      300

acaucaaccu ggguagucua cgggaccugc caccacaaaa agggcgaagc ccgaagauca      360

cggagagccg uuacccuacc gagccacucc acaagaaaau uacaaacacg aagccagacc      420

ugguuagaau caagagagua cacaaaacac cugauaagag uagagaacug gaucuuccga      480

aacccgggcu ucgcacuagc cgccgccgca aucgccuggc uccuaggauc cucaaccagc      540

caaaaaguca uuuaccucgu aaugauccuc cucauagcac cagcauacuc cauccgaugc      600

aucgguguuu ccaacagaga cuucguagaa ggcaugucgg gcggcaccug ggucgacauc      660

guccuagagc acggaggcug cgugacuguc auggcacaag acaagcccac cgucgacauc      720

gagcuaguaa ccacaacagu auccaacaug gcugaagucc gaagcuacug cuacgaagca      780

ucaaucucug acauggccag cgacagccga ugccccacac aaggagaagc cuaccuagac      840

aaacaaagcg acacccaaua cgucugcaag cgcacccuag ucgaccgcgg auggggaaac      900

gggugcggac ucuucgggaa gggaagccua guaacaugcg ccaaauucgc gugcucaaag      960

aaaaugacgg gcaagaguau ccagcccgaa aacuuagaau acagaaucau gcucucgguu     1020

cacggauccc aacacagcgg caugauagua aacgacaccg gccacgaaac agacgaaaac     1080

agggcaaaag uagaaaucac ccccaacuca cccagagccg aagccacgcu cggcggcuuc     1140

gggucacucg ggcucgacug cgagccccga accggacuug acuucagcga ccuuuacuac     1200

cuaacaauga acaacaagca cugguuagug cacaaagagu gguuccacga uaucccacuc     1260

cccuggcacg caggcgcaga caccgguaca ccccacugga acaacaaaga agcacucgua     1320

gaguucaaag augcccacgc aaaaaggcaa accgucguag uauuaggcag ccaagaaggc     1380

gcaguacaca ccgcccucgc cggagcccua gaagcagaaa uggacggcgc aaaaggaaga     1440

cucuccucag gccaccucaa augccgauug aagauggaca aacuucgacu caagggaguc     1500

aguuacucau uaugcacugc agccuucacc uucaccaaaa ucccggcaga aacgcuacac     1560

ggaaccguaa ccgucgaagu ccaauaugca ggcaccgacg gcccgugcaa aguacccgcc     1620

cagauggcgg ucgacaugca aacgcugaca ccgguagguc gccucauaac cgccaacccc     1680

guuauaacgg aauccaccga aaacuccaaa augaugcucg aacuagaccc gcccuucgga     1740

gacagcuaca ucgugaucgg ugucggagaa aagaaaauaa cccaccacug gcacagaagu     1800

gggagcacaa ucggaaaagc cuucgaagcc accgucagag gcgccaaacg cauggccguc     1860

cucggcgaca cagcauggga cuucgggagc gucggcggug cccuaaacag ccucggcaaa     1920

ggcauacacc aaauauucgg agcagcuuuc aagagcuuau ucgggggcau gagcugguuc     1980

ucccaaaucc uaaucgggac ccuacucaug uggcucggac uaaacaccaa aaacggaagc     2040

auuagccuca ugugccuggc ccucgggggc guacucaucu uccugucgac cgccguaagc     2100

gcugaugucg gaugcuccgu agacuucuca aaaugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  9396
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9396
gggagaaagc uuaccaugaa gaaacgacgg ggcgcagaca cauccgucgg caucguagga       60

cuccugcuaa ccacagcaau ggcagccgaa gucacacgca ggggcucugc auacuauaug      120

uaccuagacc ggaacgacgc aggagaagcc auaucguucc caaccacacu gggaaugaac      180

aaaugcuaua uacagauuau ggaccucggc cacaugugcg acgccaccau gagcuacgaa      240

ugcccuaugc ucgacgaggg cguggaacca gacgacgucg acugcuggug caacacgaca      300

agcaccuggg uggucuacgg aaccugccac cacaagaaag gcgaggcgag acgcucccga      360

cgcgcgguca cccucccaag ccacucaacg cgcaaacuac agacaagauc ccaaacaugg      420

cucgaaagcc gcgaauacac caaacaccua auccgagucg aaaacuggau cuuccgcaac      480

cccggauucg cacuagccgc cgccgcaaua gcauggcucu uaggcucaag caccagccaa      540

aaaguaaucu accucguaau gauccuucuc aucgcaccag ccuauucaau aagaugcaua      600

ggaguuagca acagagacuu cguagaagga augucgggag gaaccugggu cgacaucguc      660

cucgagcacg ggggcugcgu caccgucaug gcucaggaca aaccaacagu cgauaucgag      720

cuagucacca cgacaguaag caacauggca gaaguacggu ccuacugcua cgaagcaucg      780

aucuccgaca uggcaucaga cucacgaugc ccaacccaag gcgaagcaua ccuagacaag      840

caaucagaca cgcaguacgu augcaagcga acacugguag acagaggcug gggcaacgga      900

ugcggccuau ucggaaaggg cucacuggua accugugcaa aauucgcaug cucaaagaaa      960

augacaggaa aaagcauaca acccgaaaac cucgaguacc guauaaugcu cucaguccac     1020

ggaagccaac auuccggcau gaucguaaac gacacaggac acgagaccga cgaaaacaga     1080

gccaaagucg aaaucacgcc caauagccca cgcgccgaag ccacccuagg aggcuucgga     1140

ucacuaggau uggacugcga accacguacc ggccuagacu ucucagaccu auacuaccua     1200

accaugaaua acaaacacug guuagugcac aaagaguggu uccacgacau accacuccca     1260

uggcacgcgg gcgcggacac aggcacaccc cacuggaaca acaaagaagc ccuaguagag     1320

uucaaagacg cacacgcaaa acggcaaacc guaguagucc ugggcagcca agaaggcgcc     1380

guacacaccg cgcuggccgg agcccucgaa gcagaaaugg acggcgcgaa aggaagacua     1440

uccuccggcc accuaaaaug ccgacucaaa auggacaagc uaaggcugaa aggcgucagc     1500

uacucccucu guaccgcagc guuuaccuuc accaaaaucc cagcagaaac ccuccacgga     1560

acagugacag uagaagucca auacgccggc acagauggcc cuugcaaggu accagcgcaa     1620

auggcggucg acaugcaaac acuaacaccg gucggccgcc ucauaacagc caaucccgua     1680

aucacagaaa gcaccgaaaa cagcaaaaug augcucgaac uagacccgcc auucggcgac     1740

agcuacaucg ugauaggagu cggcgaaaag aaaauaacac aucauuggca cagaagcggc     1800

uccacaaucg gcaaagcauu cgaagcaaca guaagaggcg ccaaacgaau ggcaguccug     1860

ggugacacag cgugggauuu cgggucaguc ggaggugccc uuaacagccu aggaaaaggg     1920

auccaccaaa uauucggggc cgcauucaaa ucacuuuucg ggggaauguc cugguucucc     1980

caaauccuca ucggaacacu ccuaaugugg cucggacuca acaccaaaaa cggcagcauc     2040

agccucaugu gccucgcacu cggaggcgua cuaauauucc ugucaacggc aguuagcgca     2100

gacguagggu gcucagucga cuucagcaaa ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  9397
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9397
gggagaaagc uuaccaugaa gaggagaggg gcggacacca gcgucggcau agucggccua       60

cuacucacga ccgcaauggc cgccgaagua acacguagag gaucggccua cuacauguac      120

cucgacagga acgacgccgg agaagccauc ucauucccca caacacuggg aaugaacaaa      180

uguuacaucc aaaucaugga cuuaggacac augugcgacg ccaccauguc auacgaaugc      240

cccaugcucg acgaaggcgu ggaacccgac gacgucgacu gcugguguaa cacaacauca      300

accuggguag ucuacggcac augccaccac aagaaaggcg aagcccgucg cagcagaagg      360

gcaguaaccc ucccaagcca cucaacgcgg aaacugcaaa cccgaaguca aaccuggcug      420

gaaucgaggg aauacacaaa acaccuaauc cgaguagaaa acuggaucuu ccgcaaucca      480

ggcuucgcac ucgccgcagc agccauagcc uggcuacucg gcagcuccac aagccaaaaa      540

guaauuuacc ucgucaugau acuacugauc gcccccgccu acuccaucag augcaucggc      600

gucucaaacc gcgauuucgu cgaaggcaug ucagguggca ccuggguaga caucguccuc      660

gaacacggag gcugcgucac cgucauggca caagacaaac ccacagucga caucgaauug      720

gucacaacca ccgucagcaa cauggccgaa guacgaagcu acugcuacga agcgucaaua      780

uccgacaugg cgagcgacuc ccgaugucca acucaaggag aagcguaucu cgacaagcaa      840

ucggacaccc aauacguaug uaaacgaacc cuaguagacc gcggaugggg caacggaugc      900

ggccuauucg gaaaaggcuc ccucgugaca ugugcaaagu uugccugcag caagaaaaug      960

acaggcaaaa gcauccaacc agaaaaccua gaauaccgaa ucauguuguc aguacacggc     1020

ucccaacaca guggaaugau agugaacgac acaggccacg aaacagacga gaaucgcgcc     1080

aaagucgaga uaaccccuaa cucaccacgc gcagaagcga cacugggagg auucgggucc     1140

cuaggccucg acugcgaacc acgcaccggc uuagacuuca gcgaucucua cuaccuaacc     1200

augaacaaca aacacuggcu cguacacaaa gaaugguucc acgauauccc acuccccugg     1260

cacgccggcg cugacacagg cacgccccac uggaacaaca aagaagcgcu uguggaguuc     1320

aaggacgccc acgcaaaaag gcaaaccguc guaguacuag gcucacaaga gggcgccgua     1380

cacaccgccc uggcaggcgc acucgaagcc gaaauggacg gcgccaaagg acggcucucc     1440

agcggacacc ucaagugccg ccuaaaaaug gacaaacuga gacuaaaagg cguaucauac     1500

agccugugca ccgccgcauu caccuucacc aaaauacccg cggaaacccu acacggcaca     1560

guaaccguag aaguacaaua cgcaggcacc gacggccccu gcaaaguacc ggcccagaug     1620

gccguagaca ugcaaacccu cacucccguc ggacgccuca ucacagcaaa ccccgucaua     1680

acagaaucca ccgaaaacag caaaaugaug cucgaacucg acccacccuu cggagacucc     1740

uacauaguaa ucggagucgg cgagaagaaa aucacccacc acuggcaucg aucaggauca     1800

acgaucggca aagcauucga agcaaccgug cggggagcaa aaaggauggc cguacucggc     1860

gacaccgccu gggacuucgg gucagucggg ggcgcccuaa acucccuggg aaaaggcauu     1920

caccaaaucu ucggugccgc cuucaaaagc cuguucgggg gcaugagcug guucagccaa     1980

auacucauag gcacacuccu gauguggcua ggccucaaca ccaagaacgg cagcaucagu     2040

uuaaugugcc uagcccuggg aggaguccuc aucuuccuca gcacagccgu aucagcagac     2100

guaggcugua gcgucgacuu cagcaaauga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9398
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9398
gggagaaagc uuaccaugcg ccggggcgca gacacaucag ugggcaucgu aggccuccug       60

cucaccaccg cgauggccgc agaggucacc cgacgcggga gcgccuacua cauguaccua      120

gaccgaaacg augccggcga agccaucucg uucccgacaa cccugggcau gaauaaaugc      180

uacauccaaa uaauggaccu aggucacaug ugcgacgcaa cuaugucuua cgaaugcccg      240

augcucgacg agggugugga accugaugac guagacugcu ggugcaacac cacaagcaca      300

ugggugguau acggaacaug ccaccacaag aaaggcgaag cacgacggag ccggcgcgca      360

gucacccuac cgagccacuc aacccgaaaa cuccaaaccc gcagccaaac augguuggaa      420

agccgcgaau acacaaaaca ccuaauccgc gucgaaaacu ggaucuuccg caacccgggc      480

uucgcccucg cagccgcagc caucgccugg cuuuuaggcu cauccaccuc acaaaaagua      540

aucuaccuag ucaugauccu acucauagcc ccugcauacu ccaucaggug cauaggcgua      600

uccaacaggg acuucgucga aggcaugagc ggcggcaccu gggucgacau cguacucgaa      660

cacgggggcu gcguaacagu cauggcacaa gacaaaccca cagucgacau cgaacucgug      720

acaaccacag ucagcaacau ggcagaagua agaucauacu gcuacgaagc auccauaagc      780

gacauggcau cugacagcag augcccaaca cagggagaag cauaccucga caaacaaagc      840

gacacacaau acgucuguaa acgaacccua guagaccgcg gauggggaaa cggcugcgga      900

cucuucggaa aaggcagccu cguaacaugc gccaaauucg ccugcagcaa gaaaaugacc      960

ggcaaaucca uacaacccga aaaccucgag uacagaauca ugcuuucagu ccacggaagc     1020

caacauuccg gcaugauagu caacgacacc ggacacgaaa ccgacgaaaa cagagcgaaa     1080

gucgaaauca cacccaacuc acccagggcg gaagcaacac ugggaggauu cgggucacuc     1140

ggucuugauu gugaaccccg aacuggacuc gacuucuccg accucuacua ccucaccaug     1200

aacaacaaac acuggcuagu ccacaaagag ugguuccacg acaucccacu acccuggcac     1260

gcaggagccg acacaggcac cccacacugg aacaacaaag aagcacuggu cgaguucaaa     1320

gacgcacacg ccaaacgcca aacugucguc guacuaggca gccaggaagg agcaguccac     1380

acagcacugg ccggagcucu agaagcagag auggacggcg cgaaaggacg ccuaagcuca     1440

ggccacuuaa aaugccgacu caaaauggac aaacuccguc uuaaaggagu cucguacuca     1500

cugugcaccg cugcauucac cuuuaccaag auccccgcgg aaacccuaca cggaaccguc     1560

acaguagaag uccaauacgc cggaacugac ggcccaugca aaguccccgc ccaaauggca     1620

guggauaugc aaacccuaac ccccguagga cgccuaauaa cagccaaccc cguuaucacc     1680

gaauccaccg aaaacucuaa aaugaugcug gaacuagauc cacccuucgg agacuccuau     1740

auaguaaucg gcgucggaga aaagaaaauc acgcaccacu ggcaccggag cggcucaacc     1800

aucggaaaag ccuuugaggc gaccguccgc ggagccaaac gcauggcagu acucggagac     1860

acagccuggg acuucgggag cgucggaggc gcccuaaacu cacucggcaa aggcauccac     1920

caaaucuucg gagccgcauu caaaucccuc uucggcggca ugagcugguu cucacagaua     1980

cuaauaggaa cccuccuaau guggcucggc cucaacacca aaaacgguag caucagccua     2040

augugccuag cacuaggcgg cguacuaaua uuccucucca ccgccgucag cgccgacgug     2100

ggcuguuccg ucgacuucuc caaaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  9399
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9399
gggagaaagc uuaccaugag aggcgccgau acuagcguag gaaucguggg acuccuacuc       60

acaacugcaa uggccgcaga agucacaaga cgaggaagcg cauacuacau guaucuagac      120

cggaacgacg ccggagaagc aaucagcuuc ccgacaacac uaggaaugaa uaaaugcuau      180

auccaaauaa uggaccuagg ccacaugugc gacgcgacca ugagcuacga gugccccaug      240

cucgaugaag gcgucgaacc agacgacguc gacugcuggu gcaacacuac aagcacaugg      300

guaguauacg ggacauguca ccacaagaaa ggagaagcca gacgcucgcg ccgcgcaguc      360

acacuccccu cacacucaac acggaaacua caaacgcgcu cgcaaaccug guuggaaucc      420

cgagaauaca ccaaacaccu aauccgcguc gaaaacugga ucuuccgcaa cccggguuuc      480

gcacucgcag ccgcagccau cgccuggcua cucggcagcu caacaagcca aaaagucaua      540

uaccuaguaa ugauacuacu gaucgcacca gccuacagca ucagaugcau cggcgucuca      600

aaccgcgacu ucgucgaagg aauguccggc ggaaccuggg uagacaucgu acuagaacac      660

ggcggaugcg ucaccgucau ggcacaagac aaacccacag uagacauuga gcucguaaca      720

acaacagucu ccaacauggc cgagguccga ucauacugcu acgaagcauc caucagcgac      780

auggccagcg acuccaggug cccgacccaa ggcgaagccu aucucgacaa acagagcgac      840

acgcaguacg ucugcaaacg aacauuaguc gaccgaggcu ggggaaacgg augcggccua      900

uucggaaaag gaagccuagu cacgugcgca aaauucgcau gcucaaagaa aaugaccgga      960

aaaucgaucc aacccgaaaa ccuggaauac cgaauaaugc ucuccguaca cgggagccag     1020

cacucaggaa ugauagucaa ugacaccggc cacgaaacag acgagaaucg agcuaaagua     1080

gagauaacac ccaacagccc cagagcagaa gcgacccucg gcggcuucgg cucccucggc     1140

uuagacugcg aaccacgcac cggccucgac uucagcgacc uauacuauuu aacaaugaac     1200

aacaaacacu ggcuagugca caaagaaugg uuccacgaca uaccacuacc auggcacgca     1260

ggggcagaca cggggacccc acacuggaac aacaaagaag cccuaguaga guucaaagac     1320

gcacacgcaa aacgacaaac aguagucgua cuugggagcc aggaaggcgc aguccacacc     1380

gcacuagccg gggcccucga agcagaaaug gacggggcaa aaggcagacu aagcagcgga     1440

caucugaaau gcagacucaa aauggacaaa cuccgccuaa aaggcguguc cuacucacua     1500

ugcaccgcag ccuucaccuu cacgaaaauc ccagcggaaa ccuuacacgg caccgucaca     1560

guggaaguac aauacgccgg caccgacgga ccaugcaaag uaccagcaca gauggccguc     1620

gauaugcaaa cacugacgcc cgucggacga cugaucacug caaacccggu aaucaccgaa     1680

agcacagaaa acucaaaaau gaugcucgag cucgacccuc ccuucggaga cagcuacauc     1740

gucaucggag ucggagaaaa gaaaauaacu caucacuggc accgcagcgg aucaaccauc     1800

ggcaaagccu ucgaagcuac agucagaggc gcaaagagaa uggcgguacu aggcgacaca     1860

gcaugggacu ucggaucagu gggaggcgca cugaacagcc ucggaaaggg gauacaccaa     1920

aucuucggcg ccgcauucaa aagccucuuc ggcggcauga gcugguuuuc ccagauacua     1980

aucggaacgc uccuaaugug gcuagggcua aacaccaaaa acggcagcau aagccugaug     2040

ugccucgcgc ucggaggcgu ccucaucuuc cuaucaaccg cagucucggc agauguaggc     2100

ugcucagucg acuucagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9400
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9400
gggagaaagc uuaccauggg ggccgacacu uccguaggca ucgucggacu acuccugacc       60

acggccaugg ccgccgaagu aacccgccgc ggcagcgcau acuacaugua ccucgaccgc      120

aacgacgccg gggaagcuau aucguuccca acaacccucg gaaugaacaa augcuacaua      180

caaaucaugg accucggcca caugugcgac gcaaccauga gcuacgaaug uccaaugcug      240

gacgaagggg uagaaccaga cgacguggac ugcuggugca acaccacaag caccuggguc      300

gucuacggaa ccugccacca caagaaagga gaggcccgac gaagucgccg cgcagucacc      360

cucccaagcc acucuacacg aaaacuucaa acccggucac aaacauggcu cgaaucaaga      420

gaauacacca aacaccucau ccgagucgaa aacuggaucu ucagaaaccc cggauucgcc      480

cucgcagcgg ccgccauagc guggcuacua ggcucaagca ccucacaaaa agucaucuac      540

cuggucauga uccuccuaau agcacccgcc uacagcaucc gcugcaucgg cgucucaaac      600

agagacuucg uagaaggaau gagcggagga acauggguag acauagugcu cgaacaugga      660

ggcugcguaa ccgucauggc ccaagacaaa ccaaccgucg acauagaacu ggucacuaca      720

accguaucua acauggccga gguaagauca uacugcuacg aagccuccau cuccgacaug      780

gccagugaca gccgaugccc aacgcaaggc gaagccuauc ucgacaaaca auccgacacc      840

caauaugucu gcaagcgcac acucguagac cgcggcuggg gaaacggcug cggccucuuc      900

ggcaaaggca gccuaguaac cugcgcaaaa uucgcaugcu ccaagaaaau gacaggaaaa      960

uccauccaac ccgaaaaccu cgaauaccgc aucaugcucu ccgugcacgg cagccaacac     1020

uccggaauga ucguaaacga caccggacac gaaacagacg aaaaccgcgc caaagucgaa     1080

auaacaccaa acuccccacg cgcggaagcc acacuaggcg gcuucggcuc ccuaggccuc     1140

gacugcgaac ccagaacagg acucgacuuc agcgaccucu auuaccuaac caugaacaac     1200

aaacauuggu uaguacacaa ggaaugguuu cacgacaucc cccuccccug gcacgcaggc     1260

gcagauacag gaacaccaca cuggaacaac aaagaagccc ucguagaauu uaaagacgcc     1320

cacgcuaaac ggcaaacagu aguaguccua ggcagccaag agggggccgu acacaccgca     1380

cucgcaggcg cccucgaagc ugaaauggau ggcgccaaag gcaggcucuc aucgggucac     1440

cucaaaugcc gacuaaaaau ggacaaacua cgucuaaaag gggucucuua cucacucugu     1500

acggcggccu ucaccuucac caaaauacca gcagagacac uacacggaac cgucacaguc     1560

gagguccaau acgccggcac agacggacca ugcaaagucc cugcacaaau ggccgucgac     1620

augcaaaccc ucacaccagu aggacgccuc aucacggcca accccgucau cacagaaagc     1680

accgaaaacu ccaaaaugau gcucgaacua gaucccccgu ucggagacag cuacaucgua     1740

auaggcgucg gagagaagaa aauaacacac cacuggcacc gauccggauc cacaauaggc     1800

aaagccuucg aagcaaccgu cagaggcgca aaacgaaugg ccguacuggg agacacagcc     1860

ugggacuucg gaagcguggg cggagcacuc aacagccuug gcaaaggaau ccaccaaauc     1920

uucggcgcag cauucaaauc acucuucgga ggcauguccu gguucucaca aauacugaua     1980

gggacacucc ugaugugguu aggucucaac accaaaaacg gcagcauuuc ucucaugugc     2040

cucgcccucg gaggaguacu caucuuccuc uccacagcgg ucagcgcaga cgucggaugc     2100

uccgucgacu uuucgaaaug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9401
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9401
gggagaaagc uuaccauggc cgacaccagc gucggaaucg ucgggcuccu auugaccacc       60

gccauggccg ccgaagucac ccgccgcgga agcgccuacu acauguaccu agaccgaaac      120

gacgcaggcg aagccaucag cuuccccaca acacuaggca ugaacaaaug cuacauucaa      180

aucauggacc uaggccacau gugcgacgca accaugucau acgagugucc caugcuagac      240

gaaggaguag aaccagacga uguagacugc uggugcaaca ccaccuccac augggucguc      300

uacggaaccu gccaccacaa aaagggcgag gcacgacgau cacgccgcgc cgucacucua      360

ccaucacacu caacucgaaa acuacaaacc cgcucccaaa caugguuaga aagccgcgag      420

uauacgaaac accuaauccg aguagaaaau uggauauucc ggaacccagg cuucgcacug      480

gcagccgccg caaucgcaug gcuucuggga uccucaacgu cacaaaaagu caucuaccuc      540

gucaugauac ugcucauagc accagcauau uccauccgcu guaucggggu auccaacagg      600

gacuucguug aaggcauguc aggagggaca ugggucgaca ucguccucga acacggaggc      660

ugcgucacag ucauggcaca agacaaaccc accguagaca ucgagcucgu caccacaaca      720

gucagcaaca uggccgaagu ccgcagcuac ugcuacgaag caucaaucag cgauauggcc      780

agcgacucaa ggugcccuac ucaaggcgag gcuuaccuag auaaacaaag cgacacccaa      840

uacgucugca aacgcacccu aguagaccga ggauggggaa acggaugcgg ccucuucggc      900

aaaggcuccc ucgugacaug cgcaaaauuc gcaugcagca agaaaaugac aggcaaaagc      960

auacaaccag aaaaccucga auacagaauc augcugagcg uccacggguc acaacacuca     1020

ggcaugauag ugaacgacac cggccacgaa accgacgaaa accgcgcaaa aguagaaaua     1080

accccaaaca gcccacgagc cgaagccacc cuaggcggcu uuggaagccu cggccuagac     1140

ugcgagcccc gcaccggucu agacuucagc gaccucuacu accugaccau gaacaacaaa     1200

cauuggcucg ugcacaaaga gugguuccac gacauacccc uaccuuggca cgcaggagca     1260

gacaccggca caccccacug gaacaacaag gaggcacugg uagaguucaa ggacgcccac     1320

gcaaaacgac aaacaguagu cgugcuagga ucucaagaag gcgccgucca cacagccuua     1380

gccggggcac ucgaagcaga gauggacgga gccaaaggac gacuauccuc cggccaucuc     1440

aaaugccgcc ucaaaaugga caagcuccga cuaaaaggcg uaagcuacag ccuuugcacg     1500

gcagcauuca cguucacgaa aauucccgcc gaaacacucc acggcacagu aaccgucgaa     1560

guacaauacg ccggcacaga ugggcccugc aagguccccg cccaaauggc aguugacaug     1620

caaacccuca cccccguagg ccggcugauc accgccaacc cagucaucac cgaauccacc     1680

gagaacagca aaaugaugcu cgaacucgau cccccuuucg gagacuccua cauagucauc     1740

ggaguaggag aaaagaaaau cacccaccac uggcaccgca gcggcuccac cauaggaaaa     1800

gcauucgaag cuaccguccg cggggccaaa cgcauggccg uacucggcga cacggcgugg     1860

gacuucggcu cgguuggagg agcccuaaau agccucggca aaggaaucca ccaaauauuc     1920

ggagcggcuu ucaaaucccu uuucgguggc augucauggu uuagccaaau ucucaucgga     1980

acacugcuca uguggcuggg cuuaaacacc aaaaauggau caaucucgcu caugugccua     2040

gcacuaggag ggguccuaau auuccuguca accgcaguga gcgccgacgu aggcugcagc     2100

gucgacuucu caaaaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9402
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9402
gggagaaagc uuaccaugga cacaucaguu ggcaucgucg ggcugcuacu caccacggcc       60

auggccgcag aagugacaag aagaggaagc gcauacuaca uguaucugga ucgcaacgac      120

gccggcgaag cgaucagcuu cccaacgacc cucggaauga acaaaugcua cauacagauc      180

auggaccuag gacacaugug cgacgcgacc auguccuacg agugcccaau gcuagacgaa      240

ggcgucgagc cagacgacgu cgacugcugg uguaauacga ccucaaccug gguagucuac      300

gguacaugcc accacaagaa aggcgaagca cgaagaucaa gacgagccgu cacccuaccc      360

ucacacagca cuagaaaacu ccaaacccga agucaaacau ggcuagaaag ucgagaauac      420

acgaaacacc ugauccgcgu agaaaacugg auauuccgaa acccaggcuu cgcgcucgcc      480

gcagccgcca uagccuggcu ccucggcagc agcaccaguc agaaggucau auaucucgug      540

augauccucc ucaucgcccc ugccuacuca auaagaugca ucggcguaag caaccgggac      600

uucguggaag gaaugucugg aggcaccugg guagacauag uccucgaaca cggaggcugc      660

guaacuguaa uggcccaaga caaaccuaca gucgacauug aacuggucac caccaccgua      720

uccaacaugg ccgaagugcg cagcuacugc uacgaagcca gcaucucaga cauggcgagu      780

gacagccgau gccccacuca aggcgaagcc uaccuagaca aacaaagcga cacacaauac      840

gucugcaaac gaacacucgu cgaucgugga ugggguaacg gaugcggccu cuucggcaaa      900

gguucccuag ucacuugcgc aaaauucgcc ugcagcaaaa agaugacagg aaagagcaua      960

caacccgaaa accuagaaua ccgcaucaug cugagcgucc acggcagcca gcacagcgga     1020

augauaguaa acgacacagg acacgaaacc gacgaaaaua gggcgaaagu cgaaaucacc     1080

cccaacagcc ccagagcgga agcaacacua gguggcuucg ggagccucgg auuagacugc     1140

gagccccgaa caggccucga cuucagcgac cuauacuacc uaacaaugaa caacaaacac     1200

ugguuagucc acaaagagug guuccaugac aucccccucc cauggcacgc cggcgccgac     1260

accggcacac cccacuggaa caacaaggag gccuuagucg aguucaaaga cgcccacgca     1320

aaacgacaaa cagugguagu ccuaggaagc caggaaggag ccguccacac ggcccucgca     1380

ggagcacucg aggccgaaau ggacggcgca aaaggccgcc ugagcucagg acaccucaaa     1440

ugccgccuaa aaauggacaa acuaagacuc aaaggaguaa gcuacucccu uugcacagca     1500

gccuucacau ucacgaagau cccagcagaa acacugcaug gcacagugac aguagaagua     1560

caauacgccg gcaccgacgg accaugcaaa gugcccgccc agauggccgu agauaugcag     1620

acucuaacuc ccguaggcag acuaaucacc gcaaaccccg ucaucaccga gagcacagaa     1680

aacagcaaaa ugaugcuaga gcucgauccc ccauucggag acaguuacau aguaaucgga     1740

guaggcgaga agaaaaucac ccaccacugg caccgcagug gauccacuau aggcaaagcc     1800

uuugaagcaa caguccgcgg cgcaaaaagg auggcagucc uaggagacac cgcaugggau     1860

uucggcagcg uaggcggagc acuaaacagc cucggcaaag gcauacacca aauauucgga     1920

gccgccuuca agagccuauu cggcggcaug uccugguuca gccaaauacu aaucggcacc     1980

cuccucaugu ggcucggacu gaacaccaaa aacgggucaa ucagccucau gugccuggca     2040

cugggagggg uacucauauu ccuaagcacc gcaguaucag ccgacguagg uugcagcgua     2100

gacuuuagca aaugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  9403
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9403
gggagaaagc uuaccaugac cucagucggc auaguaggac ugcuccugac aacagcgaug       60

gccgccgagg uaacucgaag aggaagcgcg uacuacaugu accucgaccg aaacgacgcg      120

ggcgaagcaa ucagcuuccc gaccacccua ggcaugaaca aaugcuacau ccaaauaaug      180

gaucugggac acaugugcga cgcaaccaug ucauacgaau gucccaugcu cgacgaagga      240

guagaacccg acgacgugga uugcuggugc aacacaacca gcaccugggu agucuaugga      300

acaugccacc acaagaaagg cgaagcccga cgauccagac gugccgucac gcuacccagc      360

cacagcacac gcaaacucca aaccagaagc caaacauggc ucgaaagccg agaauacacc      420

aaacaccuca uaagagucga aaacuggaua uuccgcaacc ccggguucgc acuagcugca      480

gcagccaucg cauggcuccu aggcuccagc acaagccaaa aagucauaua ccuugucaug      540

auccuacuca uagcaccggc cuacuccaua cgcugcauag gcgucuccaa ccgagacuuc      600

gucgaaggaa ugagcggagg caccugggua gauaucgucc uggagcacgg cggaugcgua      660

accgucaugg cgcaagacaa gccaaccgua gacaucgagc ucguaaccac cacgguuagc      720

aacauggccg aaguccgcuc cuacugcuac gaagcaagca uaagcgacau ggcaagugac      780

agcagaugcc ccacucaagg cgaagcguac cucgacaaac aaagcgacac acaauacgua      840

ugcaaacgaa cccucguaga cagaggcugg ggcaacggcu gcggccuauu cggaaagggc      900

ucccucguaa ccugcgcgaa guucgccugc ucaaagaaaa ugacaggcaa aucgauacaa      960

ccggaaaacc ucgaauacag aauaaugcug uccguccacg gaucacaaca cagcggaaug     1020

auagugaaug acacaggaca ugaaaccgac gagaaccgag caaaagucga aauaacacca     1080

aacuccccac gggccgaagc cacccucgga gguuuuggca gcuuaggccu cgacugcgag     1140

ccacgaacgg gccucgacuu cucagaccuc uacuaccuaa cgaugaacaa caaacacugg     1200

cuuguccaua aagaaugguu ccacgacaua ccccuccccu ggcacgccgg agccgacacc     1260

ggcacacccc acuggaacaa caaagaggcg cuaguagagu ucaaagacgc ccacgccaag     1320

agacaaaccg ugguaguacu agggagccaa gaaggcgcgg uacacacagc acuagccgga     1380

gcccucgaag ccgaaaugga uggagcaaaa gggcgacuca gcuccggcca ccucaaaugc     1440

cgccuaaaaa uggacaaacu aagacugaag ggcguaagcu acagccuaug cacagccgca     1500

uucaccuuca ccaaaauacc cgccgaaacc cuacacggaa cagucaccgu cgaaguccaa     1560

uacgccggua ccgacggacc gugcaaggua ccggcacaaa uggcaguaga caugcaaacu     1620

cuaacccccg uaggcagacu caucacggcc aaccccguaa uaaccgaauc uacagaaaac     1680

aguaagauga ugcucgaacu cgacccaccc uuuggcgacu ccuacaucgu cauaggaguc     1740

ggagaaaaga aaaucaccca ccacuggcac aggaguggca gcacaaucgg uaaagccuuc     1800

gaagccacag uacgcggggc aaaacgcaug gcgguccucg gcgacacagc augggacuuc     1860

gggagcguag gcggcgcgcu aaacagccuc ggcaaaggca uccaccagau cuucggcgcg     1920

gcauucaaau cacuauuugg agggaugagc ugguucucuc aaauccucau aggaaccuug     1980

cugauguggc ugggccuaaa cacgaaaaac gggagcauca gccucaugug ccuugcccua     2040

gguggcgucc uaaucuuccu uagcacagcc gucucagccg acgucggaug cagcguggac     2100

uucaguaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9404
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9404
gggagaaagc uuaccaugac cagcgucggu auaguaggcc uacuacuaac aacggccaug       60

gcagccgccg ugacacgcag aggaucugcc uacuacaugu accuagaccg caacgacgcc      120

ggcgaagcga ucuccuuccc uacuacccug ggcaugaaca aaugcuacau ccaaaucaug      180

gaccuaggac acaugugcga ugccacaaug ucauacgaau gcccuaugcu ggacgaggga      240

gucgaacccg acgacguaga uuguuggugc aacaccacaa gcacaugggu aguauaugga      300

acaugccacc acaaaaaggg cgaagcgcgc agaagccgcc gcgccgucac ccuacccagc      360

cacuccacaa gaaaacugca aacgagaagc caaaccuggc ucgaaucccg cgaauacacc      420

aaacaccuca uccgcgucga aaacuggauc uuccgaaacc ccggcuucgc gcuagccgcg      480

gccgcuaucg cguggcuccu gggaucaagu accagccaaa aaguaaucua ccuaguaaug      540

auccuccuca ucgcaccagc cuacuccauc cgaugcauug gcguaagcaa cagagacuuc      600

gucgagggca ugaguggcgg caccugggua gacaucgugc uagaacacgg aggcugcguc      660

accguaaugg cccaagacaa acccacaguc gacauagaac ucguaacaac aacgguuuca      720

aacauggcag aggugagauc guauugcuac gaagccagca ucagcgauau ggccagcgac      780

agccgcugcc cgacacaagg agaagcguac cuggacaaac aaagcgacac ccaauacguc      840

ugcaagcgaa cacucguaga ccgcgggugg ggcaacggcu gcggacuauu cggaaaagga      900

agccuuguaa ccugcgcaaa guucgccugc agcaagaaaa ugacaggcaa aagcauccaa      960

ccagaaaacc ucgaauaccg gaucaugcuc agcguccaug gcucacaaca cagcggcaug     1020

auagucaacg acacgggcca cgaaacagac gaaaaccgag caaaaguaga aauaaccccc     1080

aacuccccaa gagcggaagc cacccuaggc ggauucggau cccucggacu cgacugcgaa     1140

ccacgaaccg gacucgacuu cucagaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacaua ccacucccau ggcacgccgg cgccgacaca     1260

ggcacaccac acuggaacaa caaagaggcc cuugucgagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg ucguaguccu cggaucccaa gaaggagcgg uccacaccgc ccucgcaggc     1380

gcccuagaag ccgaaaugga cggagcgaaa ggacgccuau caucggggca ccucaagugc     1440

agacucaaaa uggacaaacu ccgacuaaaa ggcgucagcu acucacucug caccgccgca     1500

uucacguuca ccaaaauccc cgccgaaacc cuacacggaa cagugacagu agaagugcag     1560

uacgcaggaa cagacggacc augcaaaguc cccgcccaga uggcaguaga caugcaaaca     1620

cucacaccag ugggacgccu aaucaccgcc aaccccguaa ucacagaaag caccgaaaac     1680

agcaaaauga ugcuagaacu cgacccaccc uucggggacu ccuacaucgu aauaggaguc     1740

ggggaaaaga aaaucacgca ccacuggcac cgaagcgguu ccacaaucgg caaagccuuc     1800

gaagcaacag uacgcggcgc aaaacggaug gcgguccucg gagacaccgc augggacuuc     1860

ggaagcgucg gaggcgcccu aaacagucua ggcaaaggaa uacaccaaau auucggugcg     1920

gccuucaaaa gucuauucgg cggcaugagc ugguucagcc aaauccucau aggaacacuc     1980

uuaauguggc ucggccuaaa uacuaaaaac ggcucaauua gccuaaugug cuuagcacua     2040

ggaggcgugc ucaucuuccu gucaaccgcc gucuccgccg acguaggcug cagcguagac     2100

uuuagcaaau gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9405
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9405
gggagaaagc uuaccaugcg aggcgcagac acgagcguag gcaucgucgg ccugcuuuua       60

accacagcaa uggcugcagc cguaacccga cgaggaagcg cauauuacau guaccuagac      120

agaaaugacg caggagaagc aauaucguuc cccacaacac uaggaaugaa uaagugcuac      180

auccaaauca uggaccucgg acacaugugc gacgccacga ugucguacga augccccaug      240

cuagacgaag gagucgaacc agacgacgug gauugcuggu guaacacaac cucaaccugg      300

gugguauacg gcacgugcca ccacaagaaa ggcgaagcaa gacgaucaag acgagcagua      360

acucuccccu cccacagcac cagaaaacuc cagacaagau cccaaacaug gcuagaauca      420

cgagaauaca caaaacaccu gauaagaguc gaaaacugga uauucagaaa ccccggauuc      480

gcacucgccg cagcagccau agcauggcuc uuaggcagcu caacaagcca aaaaguaauc      540

uaccuaguca ugauccuacu aaucgcgccc gcguacucga uccgcugcau cggcguaucg      600

aacagagacu ucgucgaagg cauguccgga gggacguggg ucgacauagu gcuagaacac      660

ggcggaugcg ucacagucau ggcacaagac aaaccgacgg ucgacauaga gcuaguaacc      720

accacaguaa gcaacauggc agaagucaga aguuacugcu acgaagcauc cauaucagau      780

auggcaucag acuccagaug cccgacccag ggagaggccu accuagacaa acaaucagau      840

acccaauacg ucugcaaacg aacccuggua gaccgcggcu ggggaaacgg cugcggccug      900

uucggcaagg ggagcuuagu aaccugcgca aaauucgcau gcagcaagaa aaugaccgga      960

aaaagcaucc aacccgaaaa ccucgaauau cgcauaaugc ucagcgucca cggcagccaa     1020

cacagcggaa ugauagucaa cgacacaggc cacgaaacag acgaaaacag ggcaaaaguc     1080

gaaaucacac caaauagccc aagggccgaa gcaacacucg ggggcuucgg gucacuuggc     1140

cuagacugcg aacccagaac gggacuagac uucagcgacc uguacuaccu caccaugaac     1200

aacaaacacu ggcuaguaca caaagaaugg uuccacgaca uaccccuccc auggcacgca     1260

ggagccgaca ccgggacgcc acacuggaac aacaaggaag cacucgucga guucaaagac     1320

gcccacgcaa agcgccaaac aguagucgua cugggcuccc aagaaggggc aguacacacg     1380

gcacucgcag gcgcacucga agcagaaaug gacggcgcca aaggccgccu cucaagcgga     1440

caccucaaau gccgccucaa gauggacaag cuccgacuca aaggcguaag cuacagccuu     1500

ugcacagcag cauucacauu cacaaaaaua cccgcagaaa cacuccacgg cacagucaca     1560

gucgaagugc aauacgcagg aaccgacggc ccaugcaagg uaccagcaca aauggcaguc     1620

gacaugcaaa cgcuaacccc agucggccgc cucauaaccg caaacccagu aauaacugag     1680

agcacagaaa acagcaagau gaugcucgag cucgacccac ccuucggcga uagcuacaua     1740

gugauaggag ucggcgagaa aaagaucacg caccauuggc acagaagcgg aagcacaaua     1800

ggaaaagcgu ucgaagcaac agucagaggc gcaaaacgca uggccguccu cggcgacaca     1860

gcaugggacu ucggcucagu aggaggggca cucaauagcc uaggcaaagg aauacaccag     1920

auauucggag cggcauucaa aagccuguuc ggcgguauga gcugguucag ccagauacua     1980

auuggaaccc uacuaaugug gcuaggccua aacaccaaaa acggaagcau cagccuaaug     2040

ugccuagcac uuggcggagu acucauauuc cucagcacag cagucuccgc cgacguagga     2100

ugcagcgucg acuucagcaa augaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9406
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9406
gggagaaagc uuaccaugcg agggcucuca ucacgcaaac gccgcaguca cgacguccua       60

accguccaau uucuaauccu aggaaugcuc cuaaugaccg gaggggucac ccgcagaggc      120

ucggccuacu acauguaccu cgacaggaac gacgccggcg aagcaauuag cuuccccaca      180

acccuuggua ugaacaaaug cuacauacaa auaauggacc uaggacauau gugcgacgca      240

acgauguccu acgagugucc cauguuggac gaaggcguag aaccagacga cguggacugc      300

uggugcaaca ccacaagcac auggguugua uacggcacau gccaccacaa gaaaggagaa      360

gcccgccgau cacgacgugc cgucacccug cccagccaca gcacgcgcaa acuacaaacu      420

cgcucgcaaa ccuggcugga aagccgcgaa uacacaaaac accucauccg agucgagaac      480

uggauauucc gcaaccccgg cuucgcacuc gcggcagccg caauagcaug gcuucuaggg      540

ucaagcacca gccaaaaagu caucuaccuc guaaugaucc uccugaucgc ccccgccuac      600

uccaucagau gcaucggagu aucaaacaga gauuucguag aaggcauguc agguggaacc      660

uggguagaca ucguacuaga gcacggaggc ugcgucaccg ucauggcaca ggauaaaccc      720

acagucgaca ucgaacuggu cacgaccacu gucucaaaca uggccgaagu gcguagcuac      780

uguuacgaag ccagcaucag cgacauggcg agcgacaguc gaugccccac ccaaggggaa      840

gcauaccucg acaaacaaag cgacacacaa uacguaugca agcgaacacu cguagaccgc      900

ggcuggggga acggaugcgg acucuucgga aaaggcagcc uagugaccug cgccaaauuc      960

gcuuguucaa agaaaaugac cggcaaaagc auacaaccgg aaaaccucga auacagaaua     1020

augcucuccg ugcacggauc ucagcacagc ggcaugauag ucaacgacac cggccacgag     1080

accgacgaaa acagggcgaa agucgaaaua acaccgaaca gcccccgagc cgaggcgaca     1140

cucggcggcu ucgggucccu cggccuagau ugcgaacccc gcacaggccu cgacuucagc     1200

gaccucuauu aucuaacaau gaacaacaaa cacuggcuag uccacaaaga augguuccac     1260

gacauuccac uccccuggca cgcgggagcg gacacgggaa caccacacug gaacaacaaa     1320

gaagcacucg uagaguucaa agacgcacac gccaaacgac aaaccguagu aguacuagga     1380

agccaagaag gcgccgugca cacagcacua gcaggggccc uagaagccga aauggacggc     1440

gccaaaggcc gacuaagcag uggacaccua aaaugccgcc uaaaaaugga caaacuacgc     1500

cuaaaaggag ucagcuauag ccuaugcaca gccgcauuca cguucacaaa aauucccgcg     1560

gaaacgcuac acggaacugu aaccgucgaa guacaauacg ccggcaccga cggaccgugc     1620

aaggucccug cacaaauggc uguagacaug caaacacuca caccggucgg ccgucuaaua     1680

acagccaacc ccguaaucac cgaauccacc gaaaacucaa aaaugaugcu ggaacucgac     1740

ccacccuucg gagacaguua cauagucauc ggagucggag agaaaaagau cacucaccac     1800

uggcacagau caggcucaac gaucggaaaa gccuucgaag caacaguccg cggggcaaaa     1860

agaauggccg uccuaggaga cacagccugg gauuucggua gcguaggagg agcccucaac     1920

ucccuaggaa agggcauaca ccaaauauuc ggagccgccu ucaaaagccu cuucggaggc     1980

augucguggu ucucgcaaau acuaaucggc acccuccuga uguggcuagg gcuaaacacc     2040

aaaaacggau cgaucucucu caugugccua gcccucggug gcgugcuaau auuccucucc     2100

accgccguca gcgcggaugu aggaugcagc guagacuuca gcaagugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  9407
<211>  2356
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9407
gggagaaagc uuaccaugcg cggacucagc ucccgcaaac gacgcucaca cgacguacua       60

accgugcaau uccucauccu ugggaugcua cucaugacag gcgcggugac ccgacgcggc      120

agcgcauacu acauguaccu cgaccgaaac gacgcgggcg aagcaaucag cuuccccaca      180

acacucggca ugaacaaaug cuacauacaa auaauggacc ucggacacau gugcgacgcc      240

accaugucgu acgagugccc aaugcuagac gaaggcguag aacccgacga cgucgacugc      300

uggugcaaua ccaccucaac auggguaguc uacggcacau gccaccacaa gaaaggagaa      360

gcccgccgcu cuagacgcgc cgucacucuc cccagucacu caacccgaaa acuccaaacg      420

cgcucccaaa cauggcucga gucccgcgaa uacacaaaac accucauccg aguagaaaac      480

uggaucuucc gaaaccccgg auucgcacuc gccgcagcag ccauagcaug gcuccuggga      540

ucaagcacau cccagaaagu aaucuaccuc gucaugaucc uacuaaucgc ucccgcguac      600

agcauacgau gcauaggcgu cagcaacaga gacuucguag aaggaauguc gggcggcaca      660

uggguagaca ucgugcucga gcacggcgga ugcguaaccg uaauggccca agacaaaccc      720

acaguagaca ucgagcucgu aacuaccaca gucaguaaca uggccgaagu ccgcagcuac      780

uguuacgagg ccucaauuuc agacauggcc uccgacaguc gcugcccaac acaaggcgaa      840

gcguaccugg acaaacaauc cgacacccaa uacguaugca aacgcacccu cguugacagg      900

ggguggggaa auggaugcgg auuauucggu aaaggguccc uaguaaccug cgccaaauuc      960

gcaugcucaa agaaaaugac cggcaaauca auacaaccag agaaccuaga auaccgcaua     1020

augcucagcg uccacgggag ccaacauucc ggaaugaucg uaaacgacac cggacacgaa     1080

accgaugaga aucgcgcaaa aguagaaauc acaccaaacu ccccacgggc ugaggcgaca     1140

cuaggcggcu ucggcagccu aggccuagac ugcgaacccc gcaccgggcu cgacuucucg     1200

gaccuguacu accucacgau gaacaacaaa cacuggcucg uacacaaaga augguuccac     1260

gauauccccc ucccauggca cgccggcgcc gacaccggaa caccccacug gaacaacaaa     1320

gaagcccucg uagaguucaa agacgcccac gccaaaagac aaacaguggu uguacuaggg     1380

ucacaggaag gcgccguaca cacagcacuc gccggagccc ucgaagccga aauggacggg     1440

gcaaaaggua gacuaaguag cggccaccuc aagugccgac ucaaaaugga caagcugcga     1500

cugaaagggg ucaguuacuc ccuaugcacc gcugcauuca ccuucaccaa gaucccggcc     1560

gaaacccuac acggaacagu caccgucgaa gugcaguacg ccggcacaga cggccccugc     1620

aaaguacccg cgcaaauggc cgucgacaug caaacacuca cgcccgucgg acggcucauc     1680

acagccaacc ccguaaucac ggaaagcaca gagaacagca aaaugaugcu agaacuagac     1740

ccacccuucg gagacuccua cauaguaauc ggcguaggcg agaagaaaau uacacaccac     1800

uggcacaggu ccggcucaac gauugguaaa gccuucgaag caacaguccg cggugccaag     1860

cgcauggcag uccuaggcga cacagcaugg gacuucggcu cagucggcgg cgcacucaac     1920

agucugggaa agggcauaca ucagauauuc ggcgcagccu ucaagucgcu auucggaggg     1980

augagcuggu ucagccagau ccucaucgga acacugcuca uguggcuagg acuaaacacc     2040

aaaaacggua gcaucagccu caugugccua gcacuaggcg gaguccuaau auuccuuagc     2100

acggcaguca gcgccgacgu gggcugcagu gucgacuucu cuaaaugagg acuaguuaua     2160

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2220

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2340

cagagccacc agaauu                                                     2356


<210>  9408
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9408
gggagaaagc uuaccaugcg caucaucaac gcgcgcaaag aaaagaaacg acgcggagcc       60

gacaccucgg ucggaauagu cggccuccua cugacaaccg ccauggcagc cgaggucacc      120

cgacgaggcu ccgccuacua cauguaccuc gaccgcaacg acgccggaga ggccauuucc      180

uuccccacca cccucggcau gaacaaaugc uacauccaaa uuauggaccu aggacacaug      240

ugcgacgcaa caaugagcua cgaaugccca augcuagacg aagguguaga acccgacgac      300

guagacugcu ggugcaacac aacauccaca ugggucgugu acgguacaug ccaucacaag      360

aaaggcgaag cacgccgcag ccgacgcgcc guaacccucc caucccacag cacaaggaaa      420

cuucaaacac gcagccagac guggcucgaa agcagagaau acacaaaaca ccucauacga      480

guagaaaacu ggauauuucg caacccgggc uuugcccucg ccgccgcggc cauagcaugg      540

cuauuaggca gcaguacguc ccaaaaagua auuuaccuag ucaugauacu acuaauagca      600

ccagccuacu ccauaaggug caucggagug ucaaaccgcg acuucgucga aggaaugagc      660

ggaggaacau gggucgacau cguccucgaa cacggcgguu gcgucacagu cauggcccaa      720

gacaaaccca caguagacau cgaacucguc acgacaacag uauccaauau ggccgaagua      780

cgauccuacu gcuacgaggc aagcauauca gacauggccu ccgacucaag augcccaacc      840

caaggagaag ccuaccucga caaacagucc gacacucaau acgugugcaa acggacacuu      900

gucgaccgcg gguggggcaa cggauguggc cuauucggca aaggcagucu gguaaccugc      960

gcaaaauuug caugcuccaa gaaaaugaca gggaaaucca uacaacccga aaaccucgaa     1020

uacagaauca ugcucucagu ccacggguca cagcacagcg gcaugauagu aaacgacacc     1080

ggccacgaga cagacgaaaa ccgcgccaaa guagaaauaa ccccaaacag cccccgggcc     1140

gaggccaccc ugggcggguu cggcagccuc ggucucgacu gcgaaccccg aacaggucua     1200

gauuucucag accuauacua ccuaaccaug aacaacaaac auuggcucgu acauaaagag     1260

ugguuccacg acaucccacu cccauggcac gcaggcgcgg acaccgguac cccacacugg     1320

aacaacaaag aagcgcuagu cgaguucaaa gacgcacacg caaaaagaca aacagucgua     1380

guccuaggcu cccaggaagg agccguccac acggcacucg ccggagcacu cgaggcagag     1440

auggacggcg ccaaaggccg ccucagcuca ggacaccuaa aaugccgccu caaaauggac     1500

aaacucagac ucaaaggggu aucauacagc cuaugcacag ccgcauuuac guucaccaaa     1560

auaccagccg agacacucca cggcaccgua acagucgagg uccaauacgc aggaacagac     1620

ggccccugca aagucccagc ccaaauggcc gucgacaugc aaacacucac acccgugggc     1680

cgucuaauaa ccgcaaaccc cguuauaaca gaauccacag aaaacuccaa aaugaugcug     1740

gaacucgauc ccccauucgg ggacagcuac aucguaaucg gcguaggcga aaagaaaauc     1800

acccaccacu ggcaccgcuc cggaagcaca auaggcaaag cauucgaagc aaccguccgc     1860

ggagcgaaaa gaauggcugu acucggagac accgcguggg acuucggcag cgugggcggc     1920

gcacucaacu cccuaggaaa aggcauccac cagauauucg gcgcagccuu caaaucccuu     1980

uucggcggca uguccugguu cucccaaauc cuuauaggaa cccuucuaau guggcucggc     2040

cucaacacca aaaacggcuc cauaucccuc augugccucg cccucggcgg cguacuaauc     2100

uuccuaagca cagccgucag cgccgacguc ggaugcugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9409
<211>  2344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9409
gggagaaagc uuaccaugau caucaacgcc cgaaaagaaa agaaacgacg aggcgccgac       60

acaagcguag gcaucguagg acuccugcuc acuacagcca uggcagcaga agugacccgc      120

cgcggauccg ccuacuacau guaccucgac cgcaacgacg ccggagaggc aauaucauuc      180

cccaccacgc uaggaaugaa caaaugcuac auacaaauaa uggaucucgg acacaugugc      240

gacgccacca ugagcuacga auguccaaug cuagacgagg gaguugaacc cgaugacgua      300

gacugcuggu gcaacaccac aucgaccugg gucgucuacg guaccugcca ccacaagaaa      360

ggcgaagccc gacgcucacg ccgagccguc acucuaccca gccacuccac aagaaaacuc      420

caaacacgca gccaaacaug gcuagaaucc cgcgaauaca caaaacaccu aauacgagua      480

gagaacugga ucuucagaaa ccccggcuuc gcgcucgcag cggccgccau cgcauggcuc      540

uuaggcucca gcaccagcca gaaaguuauc uaccucguaa ugauccuccu caucgcccca      600

gccuacucca uccgaugcau uggaguaagc aacagggacu ucguagaagg aaugagcggc      660

ggaacauggg ucgacauagu acucgaacac ggaggcugcg uaaccguaau ggcacaagac      720

aaacccaccg uggacauaga acucgucaca accaccguuu ccaacauggc cgaagugaga      780

agcuacuguu acgaagccag caucucagac auggcuuccg auagcagaug ccccacccaa      840

ggagaggcau accucgauaa acaaucagac acccaauacg ucugcaaaag aacacucgua      900

gaccgcgguu ggggcaacgg cugcggacuc uuuggcaaag gaucccuagu uaccugcgca      960

aaauucgccu gcagcaaaaa gaugacgggc aagucaauac agcccgaaaa ucuggaauac     1020

cggaucaugc uaucggucca cggcagccaa cacagcggca ugaucgugaa cgacaccggc     1080

cacgaaaccg acgaaaacag agcaaaaguc gagauaaccc caaacucacc ccgagcagaa     1140

gccacccuag gcggauucgg cagccugggc cuagacugcg aacccagaac cggccuagac     1200

uucuccgacc uguacuaccu cacaaugaac aacaaacauu gguuaguaca caaagaaugg     1260

uuccacgaca uaccucuacc auggcacgcc ggcgcagaca ccgggacccc gcacuggaac     1320

aacaaggaag cacugguaga guucaaggac gcacacgcaa aacgccaaac ggucgucgug     1380

cucggcagcc aagaaggagc cguccacacc gcgcuagcag gagcucucga ggcggaaaug     1440

gacggcgcca aaggcaggcu cuccucaggg caccucaaau gccgccucaa aauggacaaa     1500

cuccgacuca aagguguguc cuacucucuc ugcacagcag ccuucacauu caccaagauc     1560

ccugccgaaa cacuccacgg gacagucacg guagaagucc aauacgccgg uacagacgga     1620

ccgugcaaag ucccagccca aauggccgua gacaugcaga cacuuacccc agucggccgc     1680

cucaucaccg ccaaccccgu caucacagaa agcaccgaaa acucaaaaau gaugcuggaa     1740

cucgacccac ccuucgguga cagcuacauc guaaucggag uaggagagaa gaaaauaaca     1800

caccacuggc aucgcagcgg cuccaccauc ggcaaagcau ucgaggcuac aguaagagga     1860

gccaaacgaa uggccguccu aggagacacc gcaugggacu ucgggagcgu cggcggcgca     1920

cuaaacagcc ucggaaaagg gauacaucaa aucuucggcg ccgccuuuaa aucauuauuc     1980

ggcggcauga gcugguucag ccaaauauua aucggaacgc uacucaugug gcuagggcuc     2040

aacaccaaaa acggauccau cucacuaaug ugccuggcuc ucggaggcgu ccucaucuuc     2100

cuuucaacag caguaagcgc cgacguaggc ugcugaggac uaguuauaag acugacuagc     2160

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2340

aauu                                                                  2344


<210>  9410
<211>  2341
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9410
gggagaaagc uuaccaugau caacgcccgc aaagagaaga aacgcagagg cgccgacacg       60

agcguaggaa ucgucggccu acuacucacu acagccaugg cggccgaggu cacacgcaga      120

gggucagccu acuacaugua ucucgacagg aacgacgccg gagaagcgau cucauucccc      180

accacccucg gaaugaacaa augcuacauc caaauaaugg accucgggca caugugcgac      240

gcuacaaugu ccuaugagug cccuaugcua gacgaaggcg uggaaccuga cgacguagau      300

ugcuggugca acacaacuuc aaccugggua guauacggca caugccacca caagaaagga      360

gaggcacgca ggagccggcg cgccgucacg cucccaucac acuccacgcg aaaacuucaa      420

acccggagcc aaacuuggcu agaaagcaga gaauacacca aacaccucau aagaguagaa      480

aacuggaucu uccgaaaccc cggauucgcg cucgcagccg cagccaucgc cuggcuacuc      540

ggcucgagca caagccaaaa agugaucuac cuaguaauga uacuccuaau cgcccccgcc      600

uacucaaucc gaugcaucgg aguaucaaac cgagacuucg uagaaggaau gucaggagga      660

acuugggucg auauaguacu agaacacgga ggcugcguca cggucauggc ccaagacaaa      720

ccaacuguug acaucgaauu aguaaccacg accgucagca auauggcaga ggugcgaucc      780

uacugcuacg aagccuccau cucggacaug gcaagcgacu cacgaugccc aacccaggga      840

gaggcguacc ucgacaaaca aagcgacaca caguacguau gcaaaaggac gcuagucgac      900

cgcggauggg ggaacggaug cgggcucuuu ggaaaaggcu cgcucguaac augcgccaaa      960

uucgcgugca guaaaaagau gacaggcaag agcauacaac cagaaaaccu agaauaccgg     1020

aucaugcucu ccguccacgg cucccaacac agcggaauga uagucaacga caccggacac     1080

gaaaccgacg agaacagagc aaaagucgaa auaacaccca acagcccccg cgccgaagca     1140

acccuaggag gguucgggag ccucggccug gacugcgaac cacgcaccgg acuagacuuc     1200

agcgaccuau acuaccuaac aaugaacaau aaacacuggc uaguccacaa agagugguuu     1260

cacgacauac cccugccaug gcacgcagga gcugacaccg gaaccccaca cuggaacaac     1320

aaagaagccc uggucgaguu caaggacgcc cacgccaaaa gacaaacagu agucguacuc     1380

ggcucacaag aaggcgcggu acacacagca cucgccggcg cgcucgaagc cgagauggac     1440

ggcgccaagg gcagauuaag cagcggccac cucaagugca ggcuaaaaau ggacaaacua     1500

cgacugaagg gcguaucaua cucacucugc acagcugcau ucacauucac caagauacca     1560

gcggaaacac uacacgggac aguaaccgua gaaguacaau acgccggcac cgacggcccc     1620

uguaaggucc cggcccaaau ggcagucgac augcagaccc uaaccccugu cggaagacug     1680

aucaccgcca acccaguaau cacagaauca acagagaacu caaaaaugau gcuggaacug     1740

gacccgcccu ucggagauag cuacaucgug aucggcgucg gugaaaagaa aaucacgcac     1800

cacuggcaca gaagcgggag caccauaggu aaagccuucg aggcgacagu uaggggcgcc     1860

aaacgaaugg caguccucgg cgacacagca ugggacuucg gcagcguggg cggagcacuc     1920

aacucacuag ggaaaggaau ccaccaaaua uucggcgcag cauuuaaaag ccuauucgga     1980

ggaaugagcu gguucucaca gauccuaauu ggcacacuac uaauguggcu agggcucaac     2040

accaaaaacg gaagcaucag ccuuaugugc cucgcacucg guggaguccu aauuuuucug     2100

agcaccgccg ucagugcaga cgugggaugc ugaggacuag uuauaagacu gacuagcccg     2160

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2280

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2340

u                                                                     2341


<210>  9411
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9411
gggagaaagc uuaccaugaa cgcacgcaaa gaaaagaagc gccgcggagc cgacacaucc       60

guaggaaucg uaggacuacu ccucacaacg gccauggcag ccgaaguaac cagacgcggc      120

uccgccuacu acauguaccu cgaccgaaac gacgcaggag aggcuaucuc auuccccaca      180

acccucggaa ugaacaaaug cuauauacag auaauggacc ugggccacau gugcgacgca      240

acaaugagcu acgaaugccc aauguuagau gaaggcguag aacccgacga cgucgacugc      300

ugguguaaca ccacaucaac cuggguagua uacggcacau gccaccacaa gaaaggcgaa      360

gcgcgcagaa gccgucgagc agucacacua ccaucacacu caacacgaaa acuucagacc      420

cgaucacaaa ccuggcuaga aucacgagaa uauacgaaac accucauccg aguagaaaac      480

uggaucuuca gaaaccccgg cuucgcccuu gcagcggcgg ccaucgccug gcuccuaggg      540

aguucaacca gccagaaggu aaucuaccuc gucaugaucc uccucaucgc accagccuac      600

agcauccgau gcaucggcgu cagcaacaga gacuucguag aaggaaugag cggaggcaca      660

uggguagaca uaguacucga acacggcggg ugcguaaccg ugauggcaca agauaaaccc      720

acagucgaca ucgaacuugu gaccaccacc guaagcaaca uggcagaagu aagguccuau      780

ugcuacgaag caucaauuuc ugacauggca uccgacagcc gaugcccaac gcagggagaa      840

gccuaccuag acaaacaaag cgacacccaa uacguaugca aacgaacgcu gguagaccgc      900

ggcuggggca acgguugcgg acuauucggg aaaggaagcc uagucacaug cgccaaauuc      960

gccugcagca aaaagaugac aggcaaauca auccaacccg aaaacuugga auacagaauc     1020

augcuauccg uccacggaag ccaacacucc ggcaugauag ucaacgacac gggacacgaa     1080

acagacgaaa accgugcaaa aguagaaauc accccgaaua gcccaagagc ggaagccaca     1140

cuagggggcu uuggcagccu cggccuagau ugcgaaccac gaacagggcu agauuucucg     1200

gaccuauacu auuuaaccau gaacaacaaa cacuggcuag uucacaaaga augguuccac     1260

gacauaccgc ucccguggca cgccggcgca gauacgggca ccccacacug gaacaacaag     1320

gaagcgcugg uagaguucaa agaugcucac gccaaacgcc aaaccgucgu aguacuaggc     1380

ucacaggaag gugcaguaca caccgcacua gccggagcac ucgaagccga aauggacgga     1440

gcaaaaggac gacucagcag cggacaucua aaaugucgac ucaaaaugga caaacucagg     1500

cucaagggag ucagcuacag ccuaugcacg gccgcauuca cauucaccaa aauaccggca     1560

gaaacacucc acgguaccgu aacaguagag guccaauacg ccggcaccga cggccccugc     1620

aaaguaccag cacaaauggc aguagacaug caaacccuca cccccguagg acgacuaaua     1680

acggccaacc caguaauaac ugaaagcacc gaaaacagua agaugaugcu agaacuggac     1740

ccaccauucg gugacucaua caucgucauc ggcguaggcg aaaagaaaau aacccaccac     1800

uggcaccgaa gcgggucaac cauaggaaaa gccuucgaag cgacagugcg cggagccaaa     1860

cgcauggccg uacugggcga cacagcaugg gacuucggau caguaggcgg cgcccucaac     1920

agccucggca aagggaucca ccaaaucuuc ggagccgcgu uuaaaucgcu auucggcggc     1980

auguccuggu ucucccaaau ccucaucggc acccuacuca uguggcuugg ccuaaacacc     2040

aaaaauggcu caaucagccu caugugccua gcacucggag gcguccugau cuuccuguca     2100

acagcggucu cggcagacgu cggcuguuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9412
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9412
gggagaaagc uuaccauggc acgcaaggaa aagaagcgcc gcggagcaga caccucaguc       60

ggaauagucg gccugcuacu caccaccgcc auggcggcgg aaguaacccg ccggggguca      120

gccuauuaca uguaccucga ccgcaacgac gcaggcgaag ccaucucauu ccccaccacg      180

cuaggcauga acaaaugcua cauccaaaua auggaccucg gccacaugug cgacgccacc      240

augagcuacg aaugcccaau gcuagacgaa ggcguagaac ccgacgacgu cgauugcugg      300

ugcaacacaa caucaacaug gguugucuac ggaaccugcc accauaagaa aggcgaagca      360

cgacgcagcc gcagagcagu uacacuaccc ucccacucga caagaaaauu acaaacacga      420

ucacaaaccu ggcucgaauc acgagaauac accaaacacc uaauccgcgu cgaaaacugg      480

auauuccgaa aucccggauu cgcucuagca gcagccgcga ucgccuggcu gcucggcucc      540

uccacauccc aaaaagucau uuaccucguc augauccuau uaaucgcacc ggccuacuca      600

auacgcugca uaggaguauc caacagagac uucgucgaag ggaugucagg cggcaccugg      660

guagacaucg uacuagaaca cggcggcugu gucaccguaa uggcccaaga caaaccaacc      720

gucgacaucg aacucgucac gaccaccgua agcaacaugg ccgaaguacg cuccuauugc      780

uacgaagcaa gcauaucaga uauggcaagc gacucccgcu gcccaacaca gggcgaagca      840

uaccuggaca aacaaaguga cacccaauac gucuguaagc ggacccucgu cgaccgcgga      900

uggggcaacg gcugcggccu cuucggaaaa ggaucccuag uaacaugcgc caaauucgca      960

ugcuccaaga aaaugaccgg caaguccaua caaccagaaa accuagaaua ccggaucaug     1020

cuaaguguac acggcagcca acauuccgga augauaguca acgacacagg gcacgaaacc     1080

gacgaaaacc gagcaaaagu agaaauaacc ccaaacuccc ccagagcaga agccacacua     1140

ggcggcuuug gcucucuagg ccucgacugc gaaccaagaa caggucuaga cuucucagac     1200

cuuuacuacc uaacaaugaa caacaaacac uggcucguac acaaagaaug guuccacgac     1260

aucccauugc cauggcacgc aggagcagac acaggaacac cccacuggaa caacaaagaa     1320

gcgcuagucg aguucaaaga cgcacacgcc aagagacaaa ccguaguagu ccucggcuca     1380

caagaaggug ccguccacac cgcacuagca ggcgcacuag aagccgaaau ggacggagca     1440

aaaggacgac uauccucagg acaccuaaaa ugccgucuca aaauggauaa acuaagacug     1500

aaaggcguau cauacucacu cugcacagca gccuucacgu ucaccaaaau ccccgccgaa     1560

acacuacacg gcacagucac aguagaagua caauacgccg gcaccgacgg ucccugcaag     1620

guaccagcuc aaauggcggu cgacaugcaa acccuaaccc ccguagguag acucaucacu     1680

gcaaaccccg ugaucaccga aucaaccgaa aacucaaaaa ugaugcugga acuagaccca     1740

ccguucggcg acagcuacau agucaucgga guaggggaga aaaagauaac ccaccacugg     1800

cacagaagug gaucgaccau cggcaaagcc uucgaagcaa caguucgagg cgccaaaaga     1860

auggcagucc ucggagacac cgcgugggac uucggcuccg uaggcggagc ccucaacucc     1920

cucggcaagg gaauacauca gaucuucggc gccgccuuca agagccuuuu cggaggaaug     1980

agcugguucu cacaaauccu aauaggcaca cuacucaugu ggcucggccu caacaccaaa     2040

aacgggucca ucucccuuau gugccuggca cugggcggag uacucauuuu ccucagcacu     2100

gccgucagcg ccgacgucgg cuguugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  9413
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9413
gggagaaagc uuaccaugag aaaagaaaaa aaacgccgag gagcagacac aagcgucggc       60

aucguaggau uacuacugac aaccgcgaug gcagccgaag ucacaagacg aggcucagca      120

uacuacaugu accucgaccg caacgacgca ggugaagcaa uaagcuuccc gacgacucua      180

ggaaugaaca aaugcuauau ucaaauaaug gaccuaggac acaugugcga cgcaaccaug      240

agcuacgaau gccccaugcu agacgagggu gucgaacccg acgacguaga cugcuggugc      300

aacaccacaa gcaccugggu cgucuacggc acaugccacc acaaaaaagg agaggcacgc      360

agaagcagaa gagcagucac gcuccccucc cacagcacaa gaaaacuaca aaccagaucc      420

caaaccuggc uggagucgag agaauacaca aaacaccuca uaaggguaga aaacuggauc      480

uucagaaacc cgggauucgc ccuagcggca gccgccaucg cauggcuccu gggcucaagu      540

acuagccaga aagugauaua ccuaguaaug auacugcuaa uagcacccgc cuacucaauc      600

cgcugcaucg gcgucucaaa ccgagacuuc gucgaaggua ugagcggcgg uacaugggua      660

gacaucguac uagaacaugg cggaugcguc accgucaugg cacaggacaa acccacagug      720

gacaucgaac uggucaccac aaccgucucu aacauggcag agguacgcuc cuacugcuac      780

gaagccucaa ucucagacau ggcgagcgac ucccgcuguc ccacccaggg cgaagccuac      840

cuggacaaac aauccgauac acaauacgug ugcaaacgca cacucguuga ccgagggugg      900

gggaacgguu gcggccucuu cggcaaaggc agccugguga ccugcgcaaa auucgcgugc      960

ucgaaaaaaa ugacaggcaa gagcauacaa ccagaaaacc uagaguaccg gaucaugcua     1020

ucaguccacg gaucgcaaca cucaggaaug auagucaacg auacaggaca ugaaaccgac     1080

gaaaaccgag cgaaaguaga aaucacaccc aacagcccgc gcgccgaagc aacacuaggg     1140

ggcuucggaa gccucggacu agacugcgaa ccacgcaccg gccuagacuu cagcgaccuu     1200

uacuaccuaa ccaugaacaa caaacacugg cuaguccaca aagaaugguu ccaugacauc     1260

ccgcuaccgu ggcacgcugg cgcagacacg gggaccccac auuggaacaa uaaagaagca     1320

cuagucgaau ucaaagacgc gcacgcaaaa cggcaaacag ucgucguacu aggcagccaa     1380

gagggagcug uccauaccgc gcuagccgga gcccuagaag cagaaaugga cggagcaaaa     1440

ggccgccuua gcuccggcca ccucaaaugu cggcuuaaga uggacaaacu cagacucaag     1500

ggcgucagcu acagccucug caccgcagcc uucacauuca ccaaaauccc ggccgaaacc     1560

cuacacggaa caguaacagu agaggugcaa uacgcgggaa cagacggucc augcaaaguc     1620

ccagcccaaa uggcuguaga caugcaaacc cuuaccccgg uagggagacu aauaacagcc     1680

aaccccguaa uuacugaauc aacagaaaac agcaaaauga ugcucgaacu cgacccaccc     1740

uucggcgaua gcuacauagu aaucgggguc ggagaaaaaa aaauaacaca ccacuggcac     1800

agaucaggau ccacaauagg aaaagcauuc gaagccaccg uuaggggagc aaaacgaaug     1860

gcaguacucg gcgacaccgc cugggacuuc ggcagcguag gcggcgcccu aaacagccuc     1920

ggaaaaggaa uccaccaaau auucggugca gcauucaaaa gccucuucgg cggcaugagc     1980

ugguucagcc aaauacucau cggcacgcuc cuaauguggc uaggacuaaa uacaaaaaac     2040

ggcaguauca gccuaaugug ccucgcgcua gggggcgucc uaaucuuccu cucaacagca     2100

gucagcgccg acgucggaug cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9414
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9414
gggagaaagc uuaccaugaa ggaaaaaaaa cgacgcggag cagacacauc agucggaauc       60

guaggccucc uccugacaac agcaauggcu gccgaaguaa cgaggcgggg aagcgcguac      120

uacauguacc ucgacagaaa cgacgcaggc gaagccaucu ccuucccaac cacauuagga      180

augaauaaau gcuacaucca aauaauggac cucggacaca ugugcgacgc cacaauguca      240

uacgaaugcc cgaugcucga ugaaggcguc gaacccgaug acguagacug cuggugcaac      300

acaacgagca caugggucgu cuacggaaca ugccaccaca aaaaaggcga ggcccgacga      360

ucacgccgag cagugacacu ccccucccac agcacccgca aauuacaaac gagaagccaa      420

acauggcucg aaagccgcga auauaccaaa caccugauca gaguggaaaa cuggauauuc      480

aggaaccccg gcuucgcccu ggcagcagca gcaauagccu ggcuacuugg caguagcaca      540

ucccagaaag ucaucuaccu cgucaugaua cuccucaucg caccugcaua cagcauccga      600

ugcaucggcg uguccaacag agacuucgua gaaggcauga gcggcggaac augggucgac      660

auaguacucg aacacggcgg cugcguaacc gugauggcuc aagacaaacc cacagucgac      720

aucgagcuag ucaccacgac cguaagcaac auggcagaag uacgcagcua cugcuaugaa      780

gccucgauau ccgacauggc aucagacucc cggugcccca cccagggaga agcauaccuc      840

gacaaacaau ccgacaccca guacguuugu aaacgcacac uagucgacag aggcugggga      900

aacggcugcg gccuauuugg caaaggcagc cuggucacau gcgcaaaauu cgccugcagc      960

aaaaaaauga ccggaaaaag cauacaacca gaaaaccugg aauaccgcau aaugcugucc     1020

guccacggaa gccaacacuc aggcaugauc guaaacgaca ccggccacga gaccgacgaa     1080

aacagagcaa aagucgaaau cacaccgaac agcccacgag ccgaagcaac ccucggaggg     1140

uucggcucgu uaggacucga cugcgaacca agaaccggac ucgacuucuc agaccucuac     1200

uaccuaacaa ugaacaacaa acacuggcuc guccacaaag aaugguucca cgacauacca     1260

cucccauggc acgccggagc cgacaccggc accccacacu ggaacaacaa agaagcccuc     1320

gucgaauuca aagacgccca cgccaaacga caaaccguag uaguccucgg aagccaagaa     1380

ggcgcuguac acacggcccu cgcaggggcg cuggaagccg aaauggaugg cgcaaaagga     1440

agacuaagcu ccggccaccu aaaaugccgu cucaagaugg acaaacugcg ccucaaaggc     1500

gucucauaua gccuaugcac cgccgcuuuc acauucacaa aaauacccgc cgaaacacuc     1560

cacggcacag uaacagucga aguacaauau gccggcacag acggcccaug caaaguccca     1620

gcccaaaugg ccgucgacau gcaaacccuc acaccagucg gccgccuaau cacagcaaac     1680

cccguaauaa cagaaagcac agaaaacuca aaaaugaugc uagaacugga cccccccuuc     1740

ggcgauagcu acauagucau cggaguuggc gaaaaaaaaa uaacacacca cuggcaccgc     1800

uccggcucca cgaucggcaa agcauucgaa gcaacgguac gaggagcaaa aagaauggca     1860

guacuaggag auacugcaug ggacuucggc uccguggggg gggcacuaaa cucccuaggc     1920

aaaggcaucc accaaaucuu cggcgcagcc uucaaaucac ucuucggcgg gauguccugg     1980

uucagccaaa uccucaucgg cacccuacua auguggcuug ggcuaaauac gaagaacgga     2040

agcauauccc ucaugugccu agcacuaggc ggcguccuca ucuuccuuag caccgcagua     2100

uccgcagacg uaggcugcug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9415
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9415
gggagaaagc uuaccaugga aaagaaaaga cgaggagcag acaccagcgu cggcaucguc       60

ggucuacuac ucacaacagc cauggccgca gaagugacca gacggggauc ggcauacuac      120

auguaccucg accguaacga cgcaggagaa gcaaucucau ucccaaccac acucggcaug      180

aacaaaugcu acauccaaau aauggacuua ggacacaugu gcgacgccac gauguccuac      240

gaaugcccga ugcuagauga aggaguggaa cccgacgacg ucgacugcug gugcaacacc      300

acgagcacgu ggguaguaua cggaacaugu caccacaaaa agggugaagc cagacgcucc      360

cgacgggcgg ucacccuucc cucacacagc acaagaaaac ugcaaacucg cagccaaaca      420

uggcuggaau cacgcgaaua cacaaaacac cuuauacggg ucgaaaacug gaucuuccga      480

aacccaggau ucgcacuagc agcggccgcc auagccuggc uacuaggcag caguacuuca      540

caaaaaguaa uauaccuagu caugauacuc cugauagccc ccgcuuacuc cauccgaugc      600

aucggaguaa gcaauagaga uuucguugaa ggaaugucag gaggaacaug gguagacaua      660

guccucgagc acggcggcug cguaaccguc auggcacagg acaaaccaac cguggacaua      720

gaacugguca caacaacugu cagcaacaug gccgagguac gcaguuacug cuacgaggca      780

uccaucuccg acauggcauc ggacagccga ugcccgaccc aaggagaggc auaccucgac      840

aaacaaagcg acacgcaaua uguauguaaa cgaaccuuag uagaccgcgg auggggcaac      900

gguugcggac ucuucggaaa aggcucccug gucacaugcg caaaguucgc cugcuccaag      960

aaaaugacgg gaaaaagcau ccaaccggaa aaccuagagu accgaaucau gcugucuguc     1020

cacggauccc agcauuccgg caugaucguc aacgacacug gacacgaaac agacgaaaac     1080

cgagccaaag uggagauaac cccaaacagc ccaagagcag aggcaacccu aggaggauuc     1140

ggaucccuag gacuggacug cgaaccgcgc acuggacucg acuucucaga ccuauacuac     1200

cuuacaauga acaacaaaca cuggcucguc cacaaagagu gguuccacga uaucccccua     1260

cccuggcacg ccggcgcaga cacaggaaca ccacacugga acaacaaaga agcccuaguc     1320

gaguucaaag acgcgcacgc caaacggcaa acaguagucg uccucggaag ccaagaaggg     1380

gcuguacaua ccgcacucgc cggcgcccua gaagccgaaa uggacggcgc gaaaggacga     1440

cuaagcucag gacaccucaa augccggcuc aaaauggaca aacucagacu caaaggaguu     1500

uccuacuccc uaugcacagc agccuuuacc uucaccaaaa uacccgcgga aacccuccac     1560

ggaaccguca cagucgaagu ccaauaugcg ggaaccgacg ggcccugcaa agugcccgcc     1620

caaauggccg ucgauaugca aacccucacc cccguaggcc gacuaaucac agccaacccc     1680

guaauaaccg agucaacaga aaacuccaaa augaugcuag aacuagaucc cccauucgga     1740

gacagcuaca uaguaaucgg cguaggcgaa aagaaaauca cccaccacug gcaccgaucc     1800

ggaagcacaa ucggaaaagc cuucgaagca accguccgug gcgccaaaag aauggccgua     1860

cuuggagaca cagcauggga cuucgggagc gucggaggag cacugaacag ccucggaaaa     1920

ggaauccacc aaaucuucgg cgcagcguuc aagagccuau ucggcgggau gagcugguuc     1980

ucacaaauac uaaucggaac acugcucaug uggcuaggcc uaaacaccaa aaacggauca     2040

aucagccuca ugugucucgc gcugggagga guacugaucu uccuaucaac agccguaagu     2100

gccgacgucg gcugcugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9416
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9416
gggagaaagc uuaccaugaa aaaaagacgg ggugccgaca ccucgguagg aauaguagga       60

cuccuacuca ccaccgccau ggcagcagaa guaacacgcc gaggcagcgc cuacuauaug      120

uaccucgaca gaaacgacgc cggcgaagcc aucuccuucc ccaccacacu cggcaugaac      180

aaauguuaca uccaaauaau ggaucucggc cacaugugcg acgcaaccau gucauacgag      240

ugcccaaugc ucgacgaagg agucgaaccc gaugacguag acugcuggug caauaccacc      300

uccacauggg ucguauacgg aacuugccac cauaaaaaag gagaagcaag acgaucacgg      360

agagccguca ccuuacccag ccauuccacc cggaaacuac aaaccagaag ccaaacgugg      420

cucgagagcc gggaauauac aaaacaccuc auaagagucg aaaauuggau auucagaaac      480

cccggauucg cccucgccgc cgcagccaua gccuggcuac ucggauccag cacaagccag      540

aaagucauau accucgucau gauacugcua auugccccag cuuauuccau ccggugcaua      600

ggcgucagua accgggacuu cguagaagga augucaggag ggaccugggu agacaucgug      660

cuagaacacg gaggcugcgu aaccgucaug gcacaagaca aaccaacagu cgacauagaa      720

cugguaacca cuacagucag caacauggcc gaaguacgau cauacugcua ugaagcauca      780

aucagcgaca uggcaagcga cagucgaugc cccacgcagg gcgaagccua ccuagacaaa      840

caaucagaca cacaauacgu gugcaaacgg acacuugucg acagagggug gggaaacggc      900

ugcggccuau ucgggaaagg cagccuagua accugcgcga aauucgcgug cucaaaaaaa      960

augaccggca aaagcaucca gccagaaaac cuagaauaca ggaucaugcu cagcguccac     1020

ggcagccagc acagcgguau gauagucaac gacacuggcc acgaaacaga cgagaaccgc     1080

gcaaaagugg aaaucacacc aaacucacca cgcgccgaag caacccucgg uggguucggc     1140

ucccuuggac uagacuguga accaagaacc ggccucgacu ucagcgaccu auacuaccuc     1200

acaaugaaca acaaacacug gcucguccac aaagaauggu uccacgacau accccuacca     1260

uggcacgcag gcgcagacac agggacucca cacuggaaca auaaggaagc gcuagucgaa     1320

uucaaagacg cccacgcaaa aagacaaacc guggucgucc ugggauccca agaaggagca     1380

gugcacaccg cccucgcagg cgcucucgaa gccgagaugg auggagcaaa aggaagacua     1440

agcagcgggc accucaaaug ccgacuaaaa auggacaaac uaagacuaaa aggcgucagc     1500

uacagcuuau guacggccgc cuucaccuuc acgaaaaucc cugccgaaac ccuacacggc     1560

accguaaccg ucgaagucca auacgccgga acagacgggc cguguaaagu ccccgcccag     1620

auggcaguag auaugcaaac acucaccccc guaggacgcc uaaucacggc aaaccccguc     1680

auaaccgaaa gcacagaaaa cucaaaaaug augcucgaac uagauccccc cuucggagac     1740

agcuacauag uaauaggagu aggagaaaaa aaaaucaccc accacuggca cagaucagga     1800

aguaccaucg gcaaggccuu cgaagccacc gucagaggag ccaaacgaau ggcgguccuc     1860

ggagacaccg ccugggacuu cggaagcguc ggcggcgcac ucaacucacu aggcaaaggc     1920

auacaccaaa ucuucggagc cgccuucaaa agccucuucg gcggaaugag cugguucucc     1980

caaauacuaa ucggcacccu acucaugugg cucggucuca acaccaaaaa cggcuccaua     2040

ucccucaugu gccuagcacu cggaggcguc cucaucuucc ugagcaccgc cgucagugcc     2100

gacguaggcu guugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  9417
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9417
gggagaaagc uuaccaugaa gaggagaggc gcagauacga guguagguau aguagggcuc       60

uugcucacga ccgcaauggc agccgaaguc acaagaaggg gcucggcaua cuacauguac      120

cuggacagga acgacgccgg cgaagcaaua ucguucccaa caacgcuagg gaugaauaaa      180

ugcuacauac agaucaugga ucuagggcac augugugacg ccacaauguc auacgaaugc      240

ccgaugcugg augaaggcgu agagcccgac gacguggacu gcuggugcaa cacgacaagc      300

acauggguag ucuacggcac augccaucac aagaaaggcg aagcgagaag aaguaggaga      360

gcaguaacac ugccaagcca cucaacccgc aagcuacaaa cccgaaguca gaccuggcua      420

gaaucaagag aguacacaaa acaccugaua agaguagaga acuggauauu ccgaaaccca      480

ggauucgccu uggcagccgc ggcaauagcc uggcuauuag gcucguccac cagccaaaag      540

gucaucuauc ucgugaugau acuacuaauc gcccccgcau acucaaucag gugcauagga      600

gucagcaacc gcgacuuugu agaggggaug ucgggcggga cauggguaga caucguacua      660

gaacacgggg gcugugucac agugauggcc caagacaaac ccacgguaga uaucgaacuc      720

gugaccacga caguuagcaa cauggcagaa guuagaucgu acugcuacga agccagcaua      780

agcgacaugg ccucagacuc gcgaugccca acgcaagggg aggcauaccu ggacaaacaa      840

ucagacaccc aauacgucug caaaagaacc cuagucgacc gaggcugggg caacgggugc      900

gggcuauucg gcaaaggcag ccugguaaca ugugcuaaau ucgcaugcag caaaaagaug      960

acaggcaaaa guauacagcc agagaaccug gaguacagga ucaugcuaag cguccacggc     1020

agccaacaca gcggcaugau cgucaacgac acaggccacg aaacugacga aaaccgagcc     1080

aaaguagaga uaacacccaa cagcccgcgc gcagaggcaa cacugggcgg guucgggagc     1140

cugggacuag acugcgaacc gagaacgggc cuggacuuca gcgaccucua cuacuuaacc     1200

augaacaaca aacacuggcu aguccauaaa gagugguucc acgacauacc acuaccaugg     1260

cacgcaggcg cagacaccgg gacaccacac uggaacaaca aagaagcccu ggucgaguuc     1320

aaggacgcac acgccaaaag acagacaguc gugguccugg gcucgcaaga aggagccguc     1380

cacaccgccc ucgcgggagc acuagaagcc gagauggacg gggccaaagg gcgacugagc     1440

ucgggacauc ucaaaugccg ccuaaagaug gauaaacucc gccucaaagg cguaucauac     1500

agcuuaugca ccgcggcauu cacauucaca aagauccccg ccgagaccuu gcacgggacc     1560

gugacaguag aagugcaaua cgccggaacg gauggaccau gcaaaguccc agcacaaaug     1620

gcagucgaca ugcaaacccu aaccccagua ggacgccuaa uaacggcaaa ccccgucauc     1680

acagaaucaa ccgaaaacuc caaaaugaug cuggaacuag acccaccguu uggcgauagc     1740

uacaucguaa ucggaguagg agaaaagaaa aucacacauc acuggcaccg aagcggcagc     1800

acaaucggca aagcauuuga agcaacagua cggggcgcaa aacgaauggc aguacuugga     1860

gacaccgcau gggauuucgg gagcguagga ggggcccuga acucgcuagg gaaaggaauc     1920

caccaaauau ucggggccgc guucaagagc cuuuucggag gaaugagcug guucagccaa     1980

auucucauag ggacgcuccu gauguggcuc gggcuaaaca ccaaaaacgg cucaaucagc     2040

uuaaugugcc uagcacuagg aggcguccua auauuccuaa gcacagccgu aagcgcagac     2100

guaggcugcu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9418
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9418
gggagaaagc uuaccaugcg ccguggugca gacaccucag uaggaaucgu aggccuucuc       60

cuaaccacag cuauggccgc agaagucaca cgacggggau cggccuacua cauguaccuc      120

gaccgcaacg acgccggcga agcaaucagc uucccaacca cccugggcau gaacaagugc      180

uacauacaaa uaauggaccu cggccacaug ugcgacgcaa cgaugucaua ugaaugcccc      240

augcucgacg aaggaguaga acccgacgac guagacugcu ggugcaacac caccagcacc      300

ugggucgucu auggaaccug ccaccacaag aaaggcgagg cccgcagguc ccgacgcgcc      360

guaacgcuac ccagccacuc aacucgcaaa cuacaaacca gaagccaaac cuggcuagaa      420

ucccgagaau acacgaaaca ccucauaaga gucgaaaacu ggauauuccg caacccaggc      480

uucgcccugg cagcagcagc aaucgccugg cuccuaggca gcagcaccuc ccaaaaagua      540

auauaccuug uaaugauacu gcucauagca cccgcauaca gcauacgaug caucggaguc      600

agcaacagag acuucgucga aggcaugagc ggaggcacau ggguagacau cguccuagaa      660

cacggagggu gcgucacggu aauggcacag gacaaaccaa cagucgacau cgaacucguu      720

accacaaccg uaagcaacau ggcagagguc agauccuacu gcuacgaagc auccauaagc      780

gacauggcau ccgacagccg augcccaacc caaggcgagg cuuaccuaga caaacaaucc      840

gacacccagu acguaugcaa gcgaacucua gucgauaggg gauggggcaa cggaugcgga      900

cucuucggca aaggcuccuu agugacaugu gcaaaauucg ccuguagcaa gaaaaugacc      960

ggcaaaagca uccaaccaga aaaccuggag uaccguauaa ugcucagcgu acacggaagc     1020

caacacagcg gcaugauagu caacgacaca ggacacgaaa cagacgaaaa ccgugccaaa     1080

guagaaauca ccccaaacag cccacgagcu gaagccaccc uaggaggcuu cggaagccug     1140

ggacuagacu gcgaaccccg caccggccuc gacuucuccg acuuauacua ccugacaaug     1200

aacaacaaac acuggcuagu acacaaagag ugguuccaug acaucccacu acccuggcac     1260

gcaggagccg acaccggcac accgcacugg aauaauaaag aagcgcucgu agaguucaag     1320

gacgcccacg ccaagcgaca aaccguggua guccugggca gccaagaagg agcaguccac     1380

accgcccuag caggagcacu agaagccgaa auggacggag caaaagggcg acucagcuca     1440

ggacaucuca aaugccgccu gaaaauggac aaacuccggc ucaagggagu cagcuacagc     1500

cugugcaccg cagccuucac cuucaccaaa auacccgcag aaacccucca cggcaccgua     1560

acaguagaag uccaauacgc aggaaccgac ggacccugca aaguaccagc ccaaauggca     1620

guagacaugc aaacacucac acccgucggu agauuaauaa cagccaaccc agucauaacc     1680

gaaucgacug aaaacaguaa aaugaugcuc gagcuagauc ccccauucgg ggacagcuac     1740

auaguaaucg gagucggcga gaaaaagauc acacaucacu ggcaccgcag cggcagcacc     1800

aucggcaagg cuuucgaagc aacaguacgc ggggcaaaac ggauggccgu ccuaggcgac     1860

acagccuggg acuucggcuc cguaggcggg gcacucaaca gccuagggaa aggcauccac     1920

caaaucuucg gagccgccuu caaaucgcug uucggaggaa ugagcugguu uagucaaaua     1980

cuuauaggca cccuacucau guggcucgga cuaaauacca aaaacgguuc caucagccuc     2040

augugccuag cccuaggcgg gguacugauc uuccucucaa ccgccgucuc cgccgacgua     2100

ggcuguugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  9419
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9419
gggagaaagc uuaccaugag aggagcugac acgagcgucg gcauagucgg acuccuccua       60

accacagcca uggcagccga aguaacacga cguggauccg ccuacuacau guaccuggac      120

agaaacgaug caggcgaagc uaucagcuuc cccaccaccc ucggcaugaa caagugcuac      180

auacagauca uggaccuagg ccacaugugc gacgcgacga ugagcuacga augcccgaug      240

cucgacgaag gcguggaacc cgacgacguc gacugcuggu gcaacacaac cagcaccugg      300

guugucuacg gcacgugcca ccacaagaaa ggagaagcac gccgaucccg cagagccgua      360

acccuacccu cacauuccac aagaaaacua caaacccgaa gccaaacaug gcuagaaagc      420

cgcgaguaca caaaacaccu aauaagagua gaaaauugga uauuccgaaa ccccgguuuc      480

gcccucgcag ccgccgcgau cgccuggcua cuaggauccu ccacuagcca aaaaguaauc      540

uaccucguca ugauacuacu cauagcccca gccuacucca uccgcugcau cggaguguca      600

aacagggacu ucguagaagg caugagcgga ggcacguggg ucgacaucgu acuagaacac      660

ggcgggugcg ucacagugau ggcccaggac aaaccaaccg uagacaucga gcuagucacg      720

accacaguaa gcaacauggc ugaagucaga ucguacugcu acgaagccuc caucuccgac      780

auggcauccg acucccgcug cccaacccaa ggcgaagcau accucgacaa acaaucggac      840

acccaauaug uaugcaaacg cacacucgua gacagaggau ggggcaacgg cugcggacua      900

uucggaaaag ggagccuagu cacuugugcc aaauucgccu gcagcaagaa aaugaccgga      960

aaaaguaucc aacccgaaaa ccuagaauac cgcaucaugc ucuccgucca uggaucccaa     1020

cacagcggca ugaucguaaa cgacaccggc cacgaaacag acgaaaaccg cgccaaaguc     1080

gaaaucaccc ccaacucccc aagagcagag gccacccugg gaggauucgg aucacuaggc     1140

cucgauugcg aaccgcggac cggccuagac uucucagacc uguauuaccu uaccaugaac     1200

aacaagcacu ggcucguaca caaggaaugg uuccacgaua uaccacuacc cuggcacgcc     1260

ggcgccgaca caggcacacc ccacuggaac aacaaagagg ccuuagucga guucaaagac     1320

gcccacgcaa agcgucaaac cguagucgua cuuggaucgc aagaaggcgc cguccacacc     1380

gcccucgcag gggcccucga agcagaaaug gacggcgcga aagggcgccu auccagcgga     1440

caccucaaau gcagacuaaa aauggauaaa cuccgccuga aaggcgucuc auacucccuc     1500

ugcaccgccg ccuucaccuu cacaaaaauc cccgcagaaa cccuccacgg gacaguaacc     1560

gucgaagucc aguacgccgg aacagacgga cccugcaaag uaccugcgca aauggcagua     1620

gacaugcaaa cccuaacccc cgugggacga cuaauaacgg caaacccagu cauaaccgaa     1680

uccacagaaa acucaaaaau gaugcucgag cucgacccac ccuucggcga cagcuacauc     1740

gucaucggag ucggagagaa aaagauaaca caccacuggc acagauccgg cagcacgauc     1800

ggcaaagcau ucgaagccac cguacgugga gcuaaacgca uggcaguccu cggagauaca     1860

gccugggacu ucggcuccgu cggaggcgcc cucaacagcc uaggcaaagg aauacaccaa     1920

auauucggug cagcauucaa gucccucuuc ggcggcauga gcugguucuc acagauccuc     1980

auugggaccc uacucaugug gcuaggucua aacaccaaaa acggcuccau cucccucaug     2040

ugccuggcac uagguggcgu acucaucuuc cucucgaccg cgguuucagc cgacgucggg     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  9420
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9420
gggagaaagc uuaccauggg cgcggacaca agcgucggga ucguaggccu ucugcucacg       60

accgccaugg cagccgaagu cacgcgacgg ggaucggccu acuacaugua ccuugacaga      120

aacgacgccg gugaagcaau auccuucccu accacccucg gaaugaacaa auguuauaua      180

cagaucaugg accucggcca caugugcgac gcaaccaugu cauacgaaug ccccaugcua      240

gacgaaggag uggagccaga cgacguggac uguuggugca acacaacgag cacaugggua      300

guuuacggaa caugccauca caagaaagga gaagcuagaa gauccagacg cgcagucaca      360

cuaccauccc acagcacgcg caaacuccag acacgcagcc aaacauggcu ggagucaagg      420

gaauacacaa aacaccucau acgagucgag aacuggaucu uccgcaaccc cggauucgca      480

cucgcagccg cagcaauagc auggcuucuc gggagcucca ccagccaaaa aguaaucuac      540

cucguaauga uacuccuaau cgcaccagcg uacuccauaa gaugcauagg cguaagcaac      600

cgagacuucg ucgaaggaau gagcggugga acguggguug acaucguacu ggaacacggg      660

gguuguguca cggucauggc acaagacaaa ccaacggugg acaucgagcu aguaacaacg      720

accgucagca acauggcaga aguccgaucc uacugcuacg aagcaucaau cagcgacaug      780

gccucagacu cccgaugccc cacccaagga gaagccuacc uagacaagca auccgacacu      840

caauacgucu gcaaaagaac gcuagucgac cguggauggg gaaacggcug cgggcuauuu      900

ggaaaaggau cucuagucac uugcgcaaaa uucgcgugua gcaagaaaau gacaggaaaa      960

agcauacaac cggaaaauuu agaauacagg auaaugcucu ccguccacgg cucccaacau     1020

agcggcauga ucgucaacga cacaggucac gaaacagacg aaaacagggc caaaguagaa     1080

aucaccccca acucaccgcg cgcagaagca acacugggcg gcuucggcuc acuaggccuc     1140

gacugcgaac cacgcacugg cuuagacuuc agcgaccucu acuaccugac aaugaacaau     1200

aaacacuggc uaguccacaa agaaugguuc cacgacauac cgcuaccaug gcaugcagga     1260

gcggacacag gcacaccaca cuggaacaac aaagaagccc ucgucgaguu caaagacgcc     1320

cacgcaaaaa gacaaacggu cguaguccua ggcucccaag agggggcagu acacacagca     1380

cucgcaggag cacucgaagc cgagauggac ggagcgaaag gccgacuauc aucgggacac     1440

cucaaaugca ggcucaaaau ggacaaacug cggcuaaaag gagucuccua cucacucugc     1500

acagcggccu ucacuuucac caaaauccca gccgaaaccc uacacggcac cgucaccgug     1560

gaaguacaau acgccggcac cgacggcccg ugcaagguac cagcccaaau ggccguagac     1620

augcagaccu ugaccccagu aggaagacua aucacagcca acccggucau aacagaaagc     1680

accgagaacu ccaaaaugau gcuggaacug gacccacccu ucggagacag cuauaucguc     1740

auaggaguug gagagaaaaa gaucacccac cacuggcacc gaagcggauc aaccaucggc     1800

aaagccuucg aagccacagu ccgaggcgca aaaagaaugg ccguccuagg cgacacggcc     1860

ugggacuucg ggaguguagg uggagcacuc aacucacucg gaaaaggcau ccaccaaaua     1920

uucggggccg ccuucaagag ccuauucgga ggcaugagcu gguucucgca aauacuaauc     1980

ggcacacugc ucaugugguu aggccuuaac acuaaaaacg gcaguaucag ccuaaugugc     2040

cucgcacucg gaggcgugcu cauauuccug agcacagcag ugagcgcgga cgugggaugc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  9421
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9421
gggagaaagc uuaccauggc agacaccagc gucggcauag uagggcuacu acucacaaca       60

gcaauggcag ccgagguaac acggcgcggc ucagccuauu acauguaccu cgacagaaac      120

gacgcaggag aagcaaucag cuuccccacc acgcuaggca ugaacaaaug cuacauacaa      180

auaauggacc uaggccacau gugcgacgcu acaaugaguu acgaaugccc aaugcuggac      240

gaaggcguag aaccagacga cgucgacugc uggugcaaca ccacuagcac cugggucguc      300

uacggaaccu gccaccauaa gaaaggggaa gcgcgccgca gccggcgagc gguaacacuc      360

cccagccaca gcacgcgcaa acuccagacc cgcagccaga cauggcuaga auccagagaa      420

uacaccaaac accuaauaag aguagagaac uggaucuucc gcaacccagg auucgcccuc      480

gccgccgcag ccauagccug guuauuaggc uccuccacaa gccagaaagu aaucuaccuc      540

guaaugauac uccuaaucgc acccgcauau uccauaagau gcauaggcgu aagcaaccgc      600

gacuucguug aagggauguc gggcggaacc ugggucgaca ucgugcucga acacggagga      660

ugcgucacgg ucauggccca agacaaaccc accgucgaca ucgaguuagu cacuacaaca      720

guguccaaca uggccgaggu acgcuccuac ugcuacgaag caagcauauc cgacauggcc      780

uccgacaguc gcugcccaac acagggagaa gcauaccucg acaaacagag cgacacacaa      840

uacguaugca aacgaacccu aguagaccga ggauggggca acggaugcgg acuauucggc      900

aaaggcuccc uagugaccug cgcaaaauuc gcaugcucaa agaaaaugac gggaaaaagc      960

auacaaccag aaaaccucga guaccgaaua augcuaucgg uccacggcag ccaacacagc     1020

ggaaugauag uaaacgauac cggacacgaa acagacgaaa acagagccaa aguagagaua     1080

acucccaaca gcccacgagc ugaagcgacu cugggaggau ucggcagccu gggccucgac     1140

ugugaaccgc gcaccggccu agacuucuca gaucuuuauu accucacuau gaauaacaaa     1200

cauuggcucg uacacaaaga augguuccau gacauaccac uaccauggca cgccggagca     1260

gacaccggca ccccacacug gaacaacaaa gaggcccucg uagaguucaa agacgcccac     1320

gcgaaacgac aaacagucgu gguucuaggc ucacaagaag gagcggucca caccgcgcua     1380

gcuggcgcac ucgaagcaga aauggacgga gcaaaaggac ggcucagcuc cggccaccuc     1440

aaaugccgac uaaaaaugga caagcuccgg cugaaaggag ucagcuacuc ccucuguacc     1500

gccgccuuca ccuucacaaa aaucccagca gaaacacuac acggcacagu aacaguagag     1560

guccaauacg cggggaccga cgguccaugc aagguaccag cccaaauggc aguagacaug     1620

cagacccuaa cgcccguagg acgccugaua accgcaaacc caguaauaac cgaaagcacc     1680

gaaaacagca aaaugaugcu ggaauuagac ccgccauucg gcgacagcua uaucgucauu     1740

ggcgucggcg aaaagaaaau cacccaccac uggcaccgca guggauccac aauaggaaaa     1800

gcauucgagg caaccguacg aggcgcaaaa cgaauggccg uccucggaga uacagcgugg     1860

gacuucggcu ccgucggcgg agcacucaac agccuaggga aagguauaca ccaaauauuc     1920

ggcgcagcuu ucaaaagccu auucggagga augagcuggu ucagccaaau acuaaucggc     1980

acacuccuca uguggcucgg acucaacacc aaaaacggga gcauaagccu caugugccug     2040

gcgcucggag gcguacucau cuuccucuca acggcaguaa gcgcagacgu gggaugcuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  9422
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9422
gggagaaagc uuaccaugga caccuccguc ggcauagugg gacuccuccu uacuaccgcc       60

auggcagcgg aaguaacccg acgcggcuca gcauacuaca uguaccugga ccgcaacgac      120

gcaggcgagg ccaucagcuu ucccaccacc cugggaauga acaaguguua cauacaaauc      180

auggaccucg gccacaugug cgacgcaacc augagcuacg aaugcccaau gcucgacgaa      240

ggaguagaac ccgacgacgu agacugcugg ugcaacacaa ccucgacaug ggucguauac      300

ggaaccugcc accacaagaa aggcgaggcc cgccguucac gccgagccgu cacacuacca      360

ucccacucca caagaaaauu acaaacgcgc ucacaaacau ggcucgaguc cagagaauac      420

accaaacacc uaauccgagu agaaaacugg aucuuccgaa accccggcuu cgcccucgcc      480

gcagccgcaa uagcguggcu ccucgguucc agcaccuccc aaaaagucau cuaccuagua      540

augauacuac uaaucgcucc agcuuacucc auaagaugca uaggcguauc caaccgggac      600

uucgucgaag gcaugagugg cggcacaugg gucgacaucg uccuagagca cgggggcugc      660

guaacaguca uggcccaaga caaaccaacg gucgacauag aacucgucac caccacaguc      720

ucaaacaugg cagaaguccg cuccuacugc uacgaagcga gcaucaguga cauggcauca      780

gacagccgcu gcccaaccca aggagaagcc uaccuagaca aacaauccga uacucaauac      840

gucugcaaaa gaacauuagu ggacagaggc uggggaaacg gcugcggccu cuuuggaaaa      900

ggcagccuag uaacaugcgc caaauucgcu ugcucuaaga aaaugaccgg caaaucaauc      960

caacccgaaa accuggagua ucgcaucaug cuaagcguac acggcagcca acacagcgga     1020

augauaguua augacaccgg ccacgaaaca gacgaaaacc gcgcaaaagu cgaaaucacc     1080

cccaacucac cccgcgcaga agccacccua ggcgguuucg gcucacucgg cuuagacugc     1140

gaaccacgua caggacuaga cuucucagac cuguacuacc ucaccaugaa uaacaagcac     1200

uggcucgucc acaaagaaug guuccacgac auaccccugc cauggcacgc gggagcggac     1260

accggcacac cccacuggaa caacaaagaa gcccuagugg aguucaaaga cgcccacgca     1320

aaacgccaaa ccguagucgu ucucggaagu caggaagggg ccguccacac agcccuagcc     1380

ggagcccucg aagcagaaau ggacggugcg aaagggcgac ucuccucugg acaccucaaa     1440

ugccgcuuaa aaauggacaa acuacggcuc aaaggagucu ccuacagccu cugcacggcc     1500

gccuucaccu ucacaaaaau ccccgccgaa acacuccacg gaacagucac aguagaaguc     1560

caauacgccg gaaccgacgg ccccugcaaa gucccagcac aaauggcagu cgacaugcaa     1620

acacucacac ccguaggcag acucaucacc gccaacccag ucauaacaga aucaacagaa     1680

aauuccaaga ugaugcugga acucgauccc ccauucggcg acucauauau cgucauagga     1740

gugggagaaa agaaaaucac acaccacugg caucgcucag gaagcaccau cggcaaagca     1800

uucgaagcua ccguccgagg agcgaaacgg auggccguac uaggcgacac ggccugggac     1860

uucggcuccg uaggcggagc ccucaauucc cuagggaaag gcauccacca aaucuucggc     1920

gccgccuuca aaucccuauu cggcggcaug uccugguuca gccaaauacu aaucggaaca     1980

cuacucaugu ggcuaggacu caacaccaaa aacggcucca ucagccucau gugccuagcu     2040

cugggcggag uccucaucuu ccucagcacc gcuguauccg ccgacgucgg cugcugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  9423
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9423
gggagaaagc uuaccaugac cagcgucggu aucguaggcc uacuacuaac gaccgcaaug       60

gcugccgaag uaacaagacg cggcagcgcg uacuacaugu accucgaccg aaacgacgca      120

ggcgaagcaa ucagcuuccc gaccacacua ggcaugaaca aaugcuacau acaaauaaug      180

gaccucgguc auaugugcga cgcaacaaug aguuacgaau gcccaaugcu cgacgaagga      240

guagagcccg acgacgucga cugcuggugc aacaccacca gcacaugggu cgucuacgga      300

acgugccacc acaagaaagg cgaagcccgc agaucccgcc gcgcagucac ccuccccagc      360

cacucaacga gaaaacucca gacccgguca caaacguggc ucgaaucccg agaguacaca      420

aaacaccuca uacgcgugga aaauuggauc uuccgcaacc ccggcuucgc acucgccgca      480

gccgccauag ccuggcuacu cggauccuca acaucccaaa aagucauaua cuuaguaaug      540

auccuacuca uagcaccagc uuacagcauc cgcugcauag gcguauccaa cagagacuuc      600

gucgaaggaa ugagcggcgg aaccugggua gacauagucc uggaacacgg gggaugcgua      660

accgugaugg cacaggacaa acccacaguc gacauagaac ucgugacaac aacgguaagu      720

aacauggcgg aaguucgcag cuacugcuac gaagccucaa ucuccgacau ggcaagcgac      780

ucacggugcc caacgcaggg cgaagccuac cuagacaaac agucagauac acaauacguc      840

ugcaaacgaa cccucguaga ccggggcugg ggcaacggau gcggccucuu cggaaaagga      900

ucacucguca caugcgcaaa guucgcaugc aguaagaaga ugacgggcaa aagcauccag      960

ccagagaacc ucgaauaccg cauuaugcuc ucaguccacg gaucccaaca cucaggcaug     1020

aucgugaacg acacaggaca cgaaacagac gaaaaccgag cgaaaguaga aauaacccca     1080

aacagcccca gagcagaagc aacccucggc ggcuucggca gucucggacu agacugcgaa     1140

ccaagaacag gccucgacuu cagcgaucug uacuaccuaa cgaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccacucccgu ggcacgcagg agcagacacg     1260

ggcacacccc auuggaacaa caaagaggcc cuagucgagu ucaaggacgc ccacgccaaa     1320

cggcagaccg ucgugguacu aggcucacaa gaaggagcag ugcacacagc acuagcagga     1380

gcacuugagg cagaaaugga cggcgccaaa ggucgacugu caagcgggca ccuaaaaugu     1440

cgccuaaaaa uggacaaacu acgccucaaa ggcguaagcu auagccucug caccgcagcg     1500

uucacguuca cgaaaauacc ggcagagacc cuacacggca caguaacggu ugaaguccaa     1560

uacgccggca ccgacgggcc augcaaaguc ccggcccaaa uggccgucga caugcaaacg     1620

cuaacaccgg ucggacgccu caucacagcc aacccgguca ucacagaaag cacagagaac     1680

aguaaaauga ugcuagaacu agacccaccc uucggcgacu cauacaucgu aauaggcgua     1740

ggagagaaga aaauaaccca ccacuggcac agaagcggca guaccauagg caaagcauuc     1800

gaagcaaccg uaagaggcgc caaacgcaug gcaguacuag gcgacaccgc cugggacuuc     1860

ggcagcguag gaggagcccu aaacucccuc ggcaaaggca uacaccaaau auucggcgca     1920

gccuucaaaa gccucuucgg cgggaugagc ugguucuccc agauacuaau aggcacccua     1980

cuuauguggc uaggacuaaa caccaaaaac ggaagcauca gccucaugug ccucgcacuc     2040

ggcggaguac uaaucuuccu cuccacagcg gucucagcag acgucggcug uugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9424
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9424
gggagaaagc uuaccaugac cucaguugga aucgucggcc uacuucugac aaccgccaug       60

gcagccgccg ucacacgcag gggcucagca uauuacaugu accuggaccg aaacgacgcc      120

ggggaggcca ucuccuuccc caccacccuc gggaugaaca aaugcuacau ccaaauaaug      180

gaccugggcc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgaggga      240

guagaacccg acgaugucga cugcuggugc aacaccacga gcaccugggu ggucuacgga      300

acgugccacc acaagaaagg cgaagcccga cgcucccgca gggcggugac ccuacccagc      360

cacagcacac ggaaacuaca aaccaggucc cagacauggc ucgaguccag agaauacacc      420

aaacaccuaa uucgcgucga aaacuggauc uucagaaacc caggauucgc ccuagccgca      480

gccgccaucg cauggcuccu cggcucaagc acaagccaaa aagucaucua ccuagucaug      540

auacugcuaa ucgcccccgc cuacucaauc cgcugcaucg gaguaucaaa ucgcgauuuc      600

guugagggaa ugagcggagg caccuggguc gacaucgucc ucgaacacgg cggcugcgua      660

acagucaugg cucaagacaa accaacagua gacaucgagc uaguaacaac caccguaagc      720

aacauggcag aaguacgguc auacugcuac gaagcaucua uaucagacau ggccucagac      780

ucacgaugcc caacucaagg ggaagcauau cucgacaaac aaagcgacac ccaauaugua      840

ugcaaacgaa cacucguuga cagaggcugg ggaaacggcu guggccucuu cggcaaaggc      900

ucccuuguca caugcgcaaa auucgccugc ucgaagaaaa ugaccggcaa aagcauacaa      960

ccagaaaacc uggaauacag aauaaugcug agcguacacg gcucgcaaca uagcggcaug     1020

auaguaaaug acaccggaca cgaaaccgac gaaaaccgag ccaaaguaga aaucaccccc     1080

aauucucccc gcgccgaagc cacucuggga ggcuucggau cccuaggccu cgacugcgaa     1140

ccaagaaccg gacuagacuu cucagaccua uacuaccuaa caaugaacaa caaacacugg     1200

cuuguccaca aagaaugguu ccacgacauc ccccuacccu ggcacgcagg cgccgacaca     1260

ggaacgcccc acuggaacaa caaagaagca cucguagagu ucaaagacgc ccacgcgaaa     1320

cgccaaaccg uagucguccu gggcucacaa gaaggagcag uccacaccgc gcucgccggu     1380

gcacucgaag cagaaaugga cggcgccaaa ggccgacuaa gcuccggcca ucuaaaaugc     1440

cgucuaaaaa uggacaaacu ccgccuaaaa ggagucagcu acucacugug uacagcagcc     1500

uucaccuuca ccaaaauccc ggccgagacg cuacacggca ccgucaccgu cgaaguucag     1560

uacgcaggga ccgacggacc cugcaaaguc cccgcccaaa uggcgguaga caugcaaacc     1620

cugaccccgg ucggaagacu uauaaccgca aaccccguaa ucaccgaauc aaccgagaac     1680

agcaaaauga ugcuggagcu agacccaccc uucggcgacu ccuacaucgu aaucggcguc     1740

ggcgaaaaga aaauaaccca ccacuggcac cgaucgggau caaccauagg aaaagcguuc     1800

gaagcaaccg uccgcggagc aaaacggaug gccgugcuag gagacacagc augggacuuc     1860

ggaagcguag gaggcgccuu aaacucacua ggcaaaggca uacaccaaau cuucggcgcc     1920

gccuucaaga gcuuauucgg aggcaugagc ugguucuccc aaauccuaau cggcacacuc     1980

cucauguggc ugggacucaa caccaaaaac gggagcauau cgcucaugug ccucgcccug     2040

gguggcgucc uaaucuuccu aucgacagcc gucuccgccg acgucggcug cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9425
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9425
gggagaaagc uuaccaugcg cggcgccgac accagcguag gcaucgucgg acuacuccuc       60

accacagcca uggcagccgc aguaacacga cgaggaucag ccuacuacau guaccuggac      120

agaaaugacg ccggcgaagc caucagcuuu ccaacaacuc ucgguaugaa caagugcuac      180

auacaaauaa uggaccucgg ccacaugugc gacgcaacaa ugagcuacga augccccaug      240

cuagacgaag gggucgagcc cgacgacgua gacugcuggu gcaacaccac cucaaccugg      300

gugguauacg gaacaugcca ccacaagaaa ggcgaagcuc gacgcagcag acgagccgua      360

acacucccaa gccacagcac aagaaaacuc caaaccagaa gccaaacaug gcucgaaucc      420

agagaauaca caaaacaccu aauuagagua gaaaacugga uauucagaaa ccccgguuuc      480

gcacucgccg ccgccgcaau agcauggcua cucggaagcu ccaccucgca aaaagugauc      540

uaccucguca ugauccuacu aaucgcacca gccuacucca ucagaugcau cggagucucc      600

aaccgagacu ucgucgaagg aaugagcggc ggaaccuggg uagacaucgu gcuagaacac      660

ggcggaugcg ucacuguaau ggcacaagac aaaccaacag ucgacaucga acucguaacc      720

acaacggucu ccaacauggc cgaaguccga uccuacuguu acgaggccag cauaucagau      780

auggcauccg acagccgaug ccccacccaa ggcgaagccu accuagacaa acaaagcgac      840

acccaauacg uaugcaaacg cacccucguu gacagaggcu ggggaaacgg augcgggcua      900

uucggcaagg gcucacucgu cacaugcgcc aaauucgccu gcagcaaaaa gaugaccggg      960

aaaagcauac aacccgaaaa ccuagaauac cgaaucaugc uaucagugca cgggucacag     1020

cauagcggca ugaucgucaa cgacaccgga cacgagacgg acgaaaacag ggccaaaguc     1080

gagaucaccc ccaacucacc gcgcgcagaa gccacgcucg gcggauucgg cucauuagga     1140

cuggauugcg aaccacguac cggccucgac uucuccgacc ucuacuaccu cacaaugaau     1200

aacaaacacu ggcugguaca caaagaaugg uuccacgaca uaccccuacc cuggcacgcc     1260

ggagccgaca caggcacacc gcacuggaac aacaaggaag cccucgucga guucaaagac     1320

gcccacgcua aaagacaaac cgucgucgua cuaggaagcc aagaaggcgc agugcacacc     1380

gcgcuggcgg gcgcacuuga agccgaaaug gauggagcaa aaggaagacu aagcuccgga     1440

caccugaaau gucgccuaaa gauggacaaa cuaaggcuaa aaggcguauc guacucacuc     1500

ugcacagcag cauucacuuu cacaaaaaua cccgccgaaa cgcuccacgg caccguaaca     1560

gucgaagucc aauacgcggg aaccgacggc ccaugcaaag uccccgccca aauggccgug     1620

gacaugcaga cccuaacccc ggugggccgc cuaauaacag caaaccccgu aaucacugaa     1680

agcaccgaaa auucaaaaau gaugcucgaa cucgacccac ccuucggaga cagcuacaua     1740

gugauaggag uaggagagaa aaagauuacc caccauuggc acagaucagg cagcacaauc     1800

ggaaaggcuu ucgaagcaac cguacgcgga gcaaaacgca uggcgguacu cggcgacaca     1860

gccugggacu ucggcagcgu cggcggcgca cuaaacuccu uaggaaaggg uauccaccaa     1920

aucuuuggcg cagccuucaa aagccucuuc ggcggcauga gcugguucuc acaaauccuc     1980

auaggcacac uccucaugug gcucggcuua aacaccaaaa acggauccau cagccuaaug     2040

ugccuggcac ucggcggcgu ccuaaucuuu cuaucaacug ccgucagugc agacguagga     2100

ugcugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  9426
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9426
gggagaaagc uuaccaugcg aggccucagc uccagaaaac gacgaucaca cgacguccuc       60

acgguacaau uccucauacu agguaugcua cucaugaccg gaggagugac acgcagaggc      120

agcgccuacu acauguaccu cgaccgcaac gacgcaggag aggccaucag cuuccccaca      180

acccuaggca ugaauaaaug cuacauccaa aucauggacc uaggccacau gugcgacgcc      240

acaaugucau acgaaugccc caugcuugac gaaggaguag aacccgacga cguagacugc      300

uggugcaaca caaccagcac cugggucgua uacggaacgu gccaccacaa gaaaggugaa      360

gcacgaagaa gccgaagggc agucacccuu cccagccacu caaccagaaa acuccaaaca      420

cgaucccaaa ccuggcuaga gagccgcgag uauacgaaac accucauccg cgucgaaaac      480

uggaucuuca gaaacccagg cuucgcgcuc gcugcagcag caauagcaug gcuccuggga      540

agcagcaccu cgcaaaaggu uauauaccua gucaugauac uacugauagc acccgccuac      600

agcauacgcu guaucggagu auccaacaga gacuucgucg aaggaauguc cggaggaaca      660

uggguagaua uagugcucga acacgguggg ugcguaaccg ucauggccca ggacaaaccc      720

accgucgaca uagaacucgu cacaaccacg guaucaaaca uggccgaagu acgaucauac      780

ugcuacgaag caagcauuag cgacauggcg uccgacucaa ggugcccaac ccaaggagaa      840

gcauaccuag acaaacaaag cgacacgcaa uacgugugca agcgaacccu uguagacaga      900

ggauggggga acggaugcgg ccucuucgga aaaggcagcc uaguaacaug cgcaaaauuc      960

gcaugcagca agaaaaugac cggaaaaucg auacaaccag agaaccucga auacagaaua     1020

augcucagcg uccacgguuc acagcacagc ggcaugauag ugaacgacac agggcacgag     1080

accgacgaaa aucgcgcuaa agucgagaua accccaaaca gcccacgagc agaagccacc     1140

cucgggggcu ucggaucacu uggacuagac ugcgaaccac gaaccggacu agauuucagc     1200

gaccucuacu accuaacaau gaacaacaaa cacuggcucg uacacaaaga augguuccac     1260

gauaucccgu uaccauggca cgcaggagcc gacacaggaa cuccccacug gaacaacaaa     1320

gaagcacucg uggaguucaa ggacgcucac gcaaaacgcc aaaccguggu gguccuagga     1380

agccaggaag gagccguuca cacagccuua gccggagcac uagaagcgga gauggacggg     1440

gccaagggcc gccugagcag uggacaccuc aagugccgac ugaaaaugga caaacuaaga     1500

cuaaaaggag ucucguacag ccuaugcaca gcagccuuca ccuucacaaa gauaccggcc     1560

gaaacacuac acggcacagu gacaguagaa guacaauacg caggaaccga cggccccugc     1620

aaaguacccg cccaaauggc aguagacaug caaacccuca cacccguagg caggcuaauc     1680

acggcgaacc caguuauaac ggaaagcacg gagaacagca aaaugaugcu agagcuggac     1740

ccaccauuug gagacucgua cauaguaauc ggagucggag aaaagaaaau cacacaccac     1800

uggcaccguu ccggcagcac gauaggcaag gcguucgaag caacagucag aggcgccaag     1860

aggauggcgg uacucggaga caccgccugg gacuucggcu cagugggcgg cgcgcuaaac     1920

ucacucggaa aaggcauaca ccaaauauuc ggagccgcau ucaaaagucu uuucggaggc     1980

augagcuggu ucagccaaau ccugauaggc accuuacuaa uguggcucgg cuuaaacacc     2040

aaaaacggaa guaucucacu caugugcuua gcccugggcg gaguucucau auuccuuagc     2100

accgcaguca gcgccgacgu ugggugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9427
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9427
gggagaaagc uuaccaugcg cggccucucu agccgcaaac gcagaagcca cgacgucuua       60

acuguacaau ucuuaauccu cggcaugcug cuaaugacag gggcaguaac acgccgagga      120

uccgcuuacu auauguaccu ggaccggaac gacgcaggcg aagccauauc cuucccaaca      180

acccugggca ugaacaaaug cuacauacaa auaauggacc uaggucacau gugcgacgcg      240

accaugagcu acgagugccc caugcucgau gaaggagucg aacccgacga cgucgacugc      300

uggugcaaca ccacgucuac cuggguaguc uacggaaccu gccaccacaa aaagggagaa      360

gcacggcgau cuaggcgagc aguaacgcua ccaucacaua gcacgcggaa acuccagaca      420

cgaucccaaa ccugguuaga aagccgagag uacaccaaac accugauaag aguagaaaac      480

uggaucuuca gaaacccagg auucgcucua gcggcagcag ccaucgccug gcuccuugga      540

ucaucaacau cucaaaaagu aaucuaccuc guaaugaucc uccucaucgc accggcauac      600

agcauacgcu gcaucggcgu cucaaacaga gacuucguag aagggaugag cggcggcaca      660

uggguagaca uaguacucga acacggcggc ugcguaaccg uaauggcgca ggacaaacca      720

accgucgaca ucgagcuagu cacgaccacc gucucaaaca uggcugaagu ccgaucauac      780

ugcuacgaag caagcaucag cgauauggcc uccgacagua gaugccccac ccaaggagaa      840

gccuaccuug acaaacaauc cgacacccaa uacguaugca agcgaacccu cgucgaccgc      900

ggcuggggca acggcugcgg ccuauucggc aaaggcagcc ucgucacaug cgccaaauuc      960

gccugcagca agaaaaugac cggaaaauca auacaaccag aaaaccucga auaccgaauc     1020

augcucagcg uccacggcag ccaacacucc ggcaugauag ucaacgacac aggccacgaa     1080

acagacgaga accgugccaa aguagagaua acacccaaca guccccgugc cgaagcaacc     1140

cucggcggcu ucggcucccu aggccuggac ugcgaaccac gaaccggacu agacuucagc     1200

gaccuauacu accuaacaau gaacaacaag cacuggcugg uacacaaaga augguuccac     1260

gacauccccc uccccuggca cgcgggcgcc gacacgggaa ccccacacug gaacaacaaa     1320

gaagcccucg ucgaguucaa agacgcgcac gcaaaaagac aaacagucgu cguauuaggc     1380

ucacaagaag gcgcaguaca cacagcccua gcuggagccc ucgaagcaga aauggacggg     1440

gcaaaaggcc gacucagcag cggacaccuc aaaugccgcc uuaaaaugga caaacuacga     1500

cuaaagggag ucagcuacuc acucugcacc gccgcauuca cguucaccaa aauccccgcc     1560

gaaacccucc acggaaccgu aacaguagaa guacaauacg caggcaccga cggucccugc     1620

aaagucccag cccaaauggc aguagacaug cagacacuaa cgccuguagg gagacuaauu     1680

acagcaaacc cugucaucac ggaaucaacc gagaacagca aaaugaugcu agaacuagau     1740

cccccauucg gagacaguua caucguaauu ggagucggag aaaagaagau aacgcaccac     1800

uggcacagaa gcggcucaac aauaggaaaa gcauucgaag ccacaguccg gggagccaag     1860

agaauggccg uacuggggga caccgcaugg gacuucggaa gcgugggcgg agcacuaaac     1920

agccuaggca aagggauaca ccaaauauuc ggcgccgccu ucaaaucacu cuucggaggc     1980

augagcuggu ucagccagau ucuuaucggc acacuacuaa uguggcucgg ccugaacacc     2040

aaaaacggua gcauaucacu caugugccuu gcccucggag guguacuaau cuuccugagc     2100

accgccguca gcgcagacgu aggaugcuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9428
<211>  2338
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9428
gggagaaagc uuaccaugcg aauaauaaac gcacgaaaag agaagaaaag aagaggcgcc       60

gacacaagcg ugggaauagu cggccuacug cuaacaacag ccauggccgc cgaagucacg      120

cgucgaggau ccgccuacua cauguaccuc gaccgaaacg acgccggcga agccauaucc      180

uucccaacaa cccucggcau gaacaaaugc uacauccaaa uaauggaccu gggacacaug      240

ugcgacgcca ccaugucaua cgaaugccca augcuagacg aaggcgucga accagacgau      300

gucgacugcu ggugcaacac aacuagcaca uggguaguau acggcacaug ccaccacaag      360

aaaggggaag cccgccgcag ccggagggcc gucaccuuac caucccacuc aacgcgcaaa      420

cuccaaaccc gaagccaaac auggcucgaa ucgcgagaau acaccaaaca ccucauccgg      480

gucgagaacu ggaucuuucg aaaccccggg uucgcccuag cggcggcggc aauagcaugg      540

cuacucggcu caucaacguc ucaaaaaguc auuuaucuag ucaugauacu gcuaaucgcc      600

ccagcguacu caauacggug caucggagua ucaaaccgcg auuucgugga gggcauguca      660

ggcggaaccu ggguagacau agugcuagag cacggaggcu guguuacugu cauggcccag      720

gacaaaccca ccguggacau cgagcuagua acaacaaccg uauccaacau ggcagaaguc      780

cguucauacu gcuaugaagc cuccaucucc gacauggcau cagauagcag augcccaaca      840

cagggcgaag cauacuuaga caaacaguca gacacgcaau acguuuguaa acgcaccuua      900

gucgacagag gcugggggaa cggaugcggg cuauucggca agggcucgcu aguaaccugc      960

gccaaauucg caugcagcaa gaagaugacc ggcaaaucca uacaaccaga aaaccucgaa     1020

uacagaauaa ugcuaucugu ccacggcagc caacacagcg gaaugauugu caacgacaca     1080

ggccacgaaa cggacgaaaa ccgagccaaa guagaaauca cacccaacuc accgcgagcc     1140

gaagcaaccc ucgggggcuu cggcucccua ggacucgacu gcgaaccccg gaccggccuc     1200

gacuucagcg accucuacua ccuaaccaug aacaacaaac auuggcuagu ccacaaagaa     1260

ugguuccacg acaucccccu uccauggcau gcaggagcag acacaggcac accccacugg     1320

aacaacaaag aagcgcuagu agaguucaaa gacgcacacg caaaacgaca aacagucguc     1380

gugcugggau cccaagaagg cgcuguccau acagcacucg caggcgcacu cgaagccgag     1440

auggauggcg caaaaggacg acuaagcucc ggacaccuca aaugcagacu gaaaauggac     1500

aaacucagac uaaaaggagu cucauacucc cuaugcaccg ccgcguucac cuucaccaaa     1560

auaccagcag aaacacucca cggaacgguc accgucgaag uacaauaugc aggcacagac     1620

ggaccgugca aagucccggc ccaaauggca gucgauaugc aaacacuaac cccagucggc     1680

cgccugauca cugcaaaccc cguaaucaca gaaagcacag aaaacuccaa aaugaugcuc     1740

gaauuggacc cacccuucgg ggauagcuac aucguaaucg gcgucggcga aaagaaaaua     1800

acacaccacu ggcaccgcag ugggagcacu auaggcaaag cauucgaagc caccguucgc     1860

ggagccaaac gcauggccgu acucggcgac acagccuggg auuucggcuc aguaggcggc     1920

gcccuaaacu cccuagggaa aggcauccau caaauauucg gagcugcguu caaaagccua     1980

uucggaggaa ugagcugguu cucccaaaua cucaucggca cacuacugau guggcucggc     2040

cuaaacacca aaaacggauc cauaagccua augugccucg cacucggcgg cgugcucauc     2100

uuccucagca cagccguaag cgcagacuga ggacuaguua uaagacugac uagcccgaug     2160

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2280

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2338


<210>  9429
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9429
gggagaaagc uuaccaugau cauaaacgca cgcaaggaaa agaaacgaag aggcgcugac       60

acaagcgucg ggauaguagg ccuccugcua accaccgcaa uggcagcaga agugacucgc      120

cguggcagcg cauacuacau guaccucgac cguaacgacg caggggaagc aauaaguuuc      180

cccaccacac ucggcaugaa caaaugcuac auccaaauca uggaccucgg ccacaugugc      240

gacgccacca ugagcuacga gugccccaug cucgacgagg gagucgaacc agacgacgua      300

gacugcuggu guaacacgac cucaaccugg gugguauacg gaaccugcca ccauaagaaa      360

ggagaagccc gccgaucccg acgcgcagua acccuccccu cacacuccac ccgaaaacua      420

caaacacgcu cacaaacaug gcuggaauca cgcgaauaca ccaaacaccu caucagagua      480

gagaauugga ucuuccgcaa ccccggcuuc gccuuagcag ccgccgccau agcauggcua      540

cucgggagcu cuaccagcca aaaggucaua uaccuaguaa ugauccugcu aaucgcccca      600

gcauacagca uacgcugcau cggcguaucc aacagagacu ucgucgaagg uauguccggc      660

ggcacauggg ucgacaucgu ccucgaacac ggaggcugcg ugaccgucau ggcccaagau      720

aaaccaaccg ucgacauaga acucgucacc accacaguau caaacauggc cgagguacgc      780

ucauacugcu acgaagcauc cauauccgac auggcaagcg acucgaggug cccuacacag      840

ggcgaagccu accuagacaa acaaucagac acacaauacg ucuguaaacg cacccuaguc      900

gacagaggau ggggaaacgg gugcggccua uucgggaagg gcagccuggu aaccugcgca      960

aaauucgccu gcagcaagaa aaugacugga aaaucgaucc aaccugaaaa ccuggaauac     1020

cgaaucaugc uaagcgucca cggaagccag cacucgggca ugaucgucaa ugacacagga     1080

cacgaaacag acgaaaaucg cgcaaaagua gaaauaaccc ccaacucgcc cagggccgaa     1140

gccacgcucg gcggcuucgg aucgcuaggc cuugacugcg agcccagaac cggacuagac     1200

uucucagauc uguacuaccu aaccaugaac aacaaacacu ggcucgugca caaagaaugg     1260

uuucacgaca uaccccuccc auggcacgcc ggcgcagaca caggcacacc ccacuggaac     1320

aacaaagaag cacucguaga guucaaagac gcccacgcaa aacgccaaac agucgucgug     1380

cuaggcuccc aagaaggagc cguccacaca gcccucgccg gcgcccuaga agccgaaaug     1440

gacggagcca aaggacgacu cucaagcggg caucucaaau gcaggcuaaa aauggauaag     1500

cucagacuaa aaggagucag cuacucacua ugcaccgccg ccuucaccuu caccaaaaua     1560

cccgcggaaa cacuacacgg aacaguaacc gucgaagucc aauacgccgg caccgauggc     1620

ccgugcaaag uccccgcuca aauggccguc gacaugcaaa cauuaacgcc cguaggccgc     1680

cucaucacag caaauccagu cauaacggaa agcacagaaa acagcaaaau gaugcucgaa     1740

cucgacccgc ccuucggaga caguuacauc gucauuggcg uaggcgaaaa gaaaauaaca     1800

caccacuggc acagaagcgg aucgaccaua ggcaaagcau ucgaagcaac cguccgaggc     1860

gccaaaagaa uggccguacu cggagauaca gcaugggacu ucggaucagu cggcggagcc     1920

cuaaacuccc ucggcaaagg uauacaccaa aucuucggag ccgccuucaa aagccucuuc     1980

ggaggcaugu cgugguucag ucaaauacuc auaggaacac uacucaugug gcuaggccua     2040

aacaccaaaa acggcucuau cagccucaug ugccuagcac uaggaggcgu ccuaaucuuc     2100

cucagcacag cgguauccgc cgacugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  9430
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9430
gggagaaagc uuaccaugau caacgcccgg aaagaaaaga aaaggagagg cgcagacaca       60

agcgucggca uagucggacu ccuacucaca accgccaugg cggccgaagu cacacggaga      120

ggaucggccu acuacaugua ccuagaccga aacgacgccg gagaagccau cagcuucccc      180

acaacacuag gaaugaacaa augcuacaua caaauaaugg accuuggcca uaugugcgac      240

gccacaauga gcuaugaaug ccccaugcuc gacgaaggag ucgagccgga cgacgucgau      300

ugcuggugca acaccacauc cacauggguc gucuacggca caugccacca caagaaagga      360

gaagccagac gcagcagacg agccguaaca cuacccagcc acucaacacg caaacuccaa      420

acccgaagcc aaaccuggcu cgagagcaga gaauauacca aacaccuaau cagggucgag      480

aacuggauau ucaggaaccc aggauucgca cuagcugccg cagcaauagc auggcuacua      540

ggcagcagca ccagccagaa agugaucuac cucguaauga uacuucucau agcacccgcc      600

uacucaauac gaugcauagg cgucagcaac cgcgacuucg ucgaaggcau gucaggugga      660

acauggguag acaucgugcu cgaacacggc ggcugcguca ccgucauggc ccaagacaaa      720

cccaccguag acauagagcu agugaccaca acaguaagca auauggccga agugagaagc      780

uacuguuacg aagccagcau cagcgacaug gcguccgacu cucgcugccc aacccaagga      840

gaagcguacc uagacaaaca aagcgacaca caauacguau gcaaaaggac ccucgucgac      900

cgaggcuggg gcaacggaug cggacuguuc ggcaaaggga gccuaguaac augcgcaaaa      960

uucgcgugcu ccaagaaaau gacaggaaaa agcauacaac cagagaaccu agaauaccgc     1020

aucaugcuaa gcguccacgg aagccaacac ucaggcauga uaguuaacga cacaggccac     1080

gagaccgaug agaaccgcgc caaaguggag aucacaccaa acuccccaag agcagaagcg     1140

acccuaggag gauucggauc gcucgggcuc gacugcgagc caagaacagg ccuagacuuc     1200

uccgaccucu acuaccuaac caugaacaau aaacauuggc uagugcacaa agaaugguuc     1260

cacgacauac cgcucccaug gcacgccggc gcugacacgg gaacgcccca cuggaacaau     1320

aaagaagcac uggucgaguu caaggacgca cacgccaaaa gacaaaccgu cguaguacuc     1380

ggaagccaag aaggcgccgu acacaccgca cuggccggag cccuagaagc agaaauggac     1440

ggcgcgaaag gaagacucag cucaggacac cugaagugcc ggcucaaaau ggacaaacuc     1500

cgacucaaag gaguaagcua cagccucugc acagccgccu ucaccuucac caagaucccg     1560

gcagaaaccc uucacggcac cgucacagua gaaguacaau acgcagguac agacggaccc     1620

ugcaaggucc ccgcacaaau ggcaguagac augcaaaccc ugacaccagu ugggagacug     1680

aucaccgcaa acccagugau caccgaauca accgaaaaca gcaaaaugau gcuagaacuc     1740

gacccaccau ucggggacag cuacauagua aucggcgugg gagaaaagaa aauaacacac     1800

cacuggcacc gcucaggcag uacuauagga aaagccuucg aggccacagu ccguggggcc     1860

aaaagaaugg cgguacuagg cgacaccgcc ugggacuucg gcagcguagg gggcgcacua     1920

aacucacucg gaaaagggau ucaccagauu uucggcgcag ccuucaaaag ccucuucgga     1980

ggaaugagcu gguucagcca aauacucaua ggaacgcuac ugauguggcu gggccucaac     2040

accaaaaacg ggucaauaag ccucaugugc cuagcccuag gcggaguccu aauauuccua     2100

uccacagcag ucagcgcaga cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9431
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9431
gggagaaagc uuaccaugaa ugcacgaaaa gaaaagaaac gacgaggagc agacacuuca       60

gucggcaucg ugggccugcu acucacaaca gcgauggccg ccgaagucac acgccgaggc      120

ucugcuuacu acauguaccu cgaucgaaac gacgccgggg aagcaaucuc guuccccacc      180

acgcugggaa ugaacaaaug uuacauacaa aucauggacc ucggccacau gugcgacgcc      240

accauguccu acgaaugccc aaugcucgac gaaggaguag agccagacga cgucgacugc      300

uggugcaaca ccaccagcac guggguaguc uacgggacgu gccaccauaa gaaaggagaa      360

gcacgcagaa guagacgagc agucacccua ccaagccacu caacccgcaa acuacaaacc      420

agaucucaaa cguggcucga aagucgcgag uacacuaaac accucauacg cgucgaaaac      480

uggaucuucc gaaaucccgg auucgcgcug gcagcagcag ccauagccug guuacugggc      540

agcuccacca gccaaaaagu aaucuaccuu gugaugaucc uacuaaucgc uccagccuac      600

agcauccgau gcauaggcgu cagcaaccga gacuucgucg agggcauguc cggcggaacc      660

uggguagaca ucguacuaga acacggcggg ugcgucacag ucauggcaca agacaaaccc      720

accgucgaca uagagcuagu aaccacgaca guaagcaaca uggcagaagu cagaagcuac      780

uguuacgaag caucaauauc cgacauggca agcgacuccc gaugccccac ccaaggcgaa      840

gcauaccuag acaaacaaag cgacacccag uacgucugca aaaggacccu agucgaccga      900

ggcuggggaa acgggugcgg acuguucggg aaaggcagcc ucguaaccug cgccaaauuc      960

gccugcucaa agaaaaugac gggcaaaagc auacaacccg aaaaccucga auacaggauc     1020

augcuaucug uccauggcag ccaacacucc ggcaugauag ucaaugauac uggacacgaa     1080

acagacgaaa accgcgccaa aguagaaauc acacccaaca gcccccgcgc agaagcaacc     1140

cucggaggcu ucggaucccu aggccucgac ugcgaaccac gcaccggccu cgacuucucc     1200

gaucucuacu accucaccau gaacaauaaa cacuggcugg uccacaaaga augguuccac     1260

gacauacccc ugccguggca cgccggcgcc gacacaggaa ccccacacug gaacaacaaa     1320

gaggcgcuag ucgaguucaa agacgcccac gcuaaacgac aaacggucgu cguccuaggc     1380

ucccaagaag gggcggucca cacagcccuc gcaggagccc uggaagccga aauggacggc     1440

gccaaaggca gacucagcuc cggccaccua aagugccgcc ucaaaaugga caaacuaaga     1500

cugaaaggcg ucagcuacag ccuaugcaca gcagcauuca cauucaccaa aauacccgcc     1560

gaaacccugc acggaacagu gacaguagaa guccaauacg ccggcaccga cgggcccugc     1620

aaaguaccag cccaaauggc cgucgacaug caaacgcuca ccccagucgg ccgacugauc     1680

accgccaacc cagucaucac cgaauccaca gagaacucaa aaaugaugcu cgaacuagau     1740

cccccauucg gcgacuccua cauaguuauc ggcguuggag aaaagaaaau cacgcaccac     1800

uggcacagga gcgguucaac aauaggaaaa gccuucgaag cgacaguccg cggagccaaa     1860

cgaauggcgg uacugggcga cacugcaugg gacuucggca gcguaggcgg cgcucucaac     1920

ucccugggaa aaggaauaca ucaaauauuc ggagccgcau uuaaaucacu guucggaggc     1980

augucauggu ucagccagau ccuaauaggc acccuccuca uguggcucgg acuaaacacg     2040

aaaaauggaa gcauaagccu uaugugccua gcacucggcg gaguccuaau auuccucagc     2100

acggccguga gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9432
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9432
gggagaaagc uuaccauggc ccgaaaagaa aaaaaacgac gaggcgccga cacauccgua       60

ggaauagucg gccuccuacu aaccacggcu auggcagccg aaguaacgcg ccgaggauca      120

gccuacuaca uguacuuaga cagaaacgac gcaggagaag caaucagcuu cccaaccacg      180

cucggaauga auaaaugcua cauccaaaua auggaccuag gccacaugug ugacgccacu      240

auguccuacg aaugccccau gcuagacgag ggcgucgagc cagacgacgu cgauugcugg      300

ugcaacacca ccagcaccug ggucguguac gggacaugcc accacaaaaa aggcgaagca      360

cgacgauccc gccgagcagu cacccugcca ucccauagca caagaaaacu ccaaacccgc      420

agccagacau ggcuagaaag ccgagaauac acaaaacacc ucauccgagu ggaaaacugg      480

aucuuucgca acccaggcuu cgcacucgca gcagccgcca ucgccuggcu ccuaggcucg      540

agcaccagcc aaaaagucau auaccucguc augauacuac ucauagcccc ggcauacucc      600

auccgaugca uuggagucag caaccgggac uucgucgaag gaaugagcgg cggcaccugg      660

guagacaucg ugcucgaaca cggaggcugc guaaccguca uggcgcagga caaacccaca      720

guagauaucg aacuagucac cacuacagua agcaacaugg cggaaguacg aagcuacugu      780

uacgaggcca gcauauccga cauggcaucg gacagcagau gccccaccca aggggaggca      840

uaccucgaca aacaaucaga cacgcaauac guaugcaaac ggacccuggu cgaucgcgga      900

uggggcaacg ggugcggccu cuucggcaaa ggcucacucg ucaccugcgc caaauucgca      960

ugcuccaaga aaaugaccgg caaaagcaua caaccagaga accuagaaua ccgaaucaug     1020

cugucagucc acgguagcca acacagcggc augaucguca acgacaccgg acacgaaacc     1080

gacgaaaaua gagccaaagu agaaaucacg cccaacagcc caagagcaga agccacccuc     1140

ggcggguucg guagccuagg ccuugacugc gagccgcgca caggacugga cuucagcgac     1200

cucuacuacc ugaccaugaa caauaaacac uggcucguac acaaagagug guuccacgac     1260

aucccccuac ccuggcacgc aggagccgac accggcacgc cacauuggaa caacaaagaa     1320

gcccucgucg aauucaaaga cgcgcaugcg aaaagacaga cagucguagu ccuaggauca     1380

caagaaggag cgguacauac agcacuagca ggagcgcucg aagcagaaau ggacggcgca     1440

aaaggacgac uaucgucagg ccaccucaaa ugccgacuca aaauggacaa acuaagacuc     1500

aaagggguca gcuacuccuu augcaccgcc gcauucacau ucaccaaaau ccccgcagaa     1560

accuuacacg gaaccgugac cgucgaagua caauacgcag gaaccgaugg acccugcaaa     1620

gucccagcac agauggccgu agauaugcaa acccugacac cggucggccg acuaauaaca     1680

gcaaaccccg uaaucacuga aucaaccgaa aacucaaaaa ugaugcuaga acuugaccca     1740

cccuucggcg acagcuacau cguaaucggc guaggcgaga aaaaaauuac acaccacugg     1800

caccgauccg gaagcacgau cggcaaggca uucgaagcga cgguccgagg agccaaacga     1860

auggcagucc ucggcgacac agcgugggac uucggcagcg ucggcggcgc ucucaacagc     1920

cuaggcaaag gcauccacca gaucuucggc gccgccuuca aaucccuauu cggcggcaug     1980

ucaugguuca gccaaauccu aauaggcaca cuccucaugu ggcucggccu caacacaaaa     2040

aacggcucaa uaagccuaau gugccucgcc cucggcggcg uucucauuuu ccucagcacc     2100

gcagucagcg cggacugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9433
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9433
gggagaaagc uuaccaugcg gaaagaaaag aaacgccgag gcgcagacac gagcguagga       60

auagucggcc uacuccugac gacagcaaug gcagcagaag uaacacgccg uggaagcgcg      120

uacuacaugu accucgaccg gaacgacgca ggagaagcca ucagcuuccc aaccacccuc      180

ggaaugaaca aaugcuacau acaaauaaug gaccuaggac acaugugcga cgccaccaug      240

uccuacgagu gccccaugcu agacgaagga guagaaccag acgacguaga cugcuggugc      300

aacacaacau ccacgugggu aguauacgga acaugccacc acaagaaagg cgaagcacgc      360

cggagccgac gagccgucac gcugccaucc cacagcacac gaaaacuaca aacccggucc      420

cagaccuggc uagaaucaag agaauacaca aaacaccuca uacggguaga gaacuggaua      480

uucagaaacc ccggguucgc acucgcagcc gccgcaauag ccuggcuccu gggcucaagc      540

accucccaaa aagugauaua ccuagucaug auacugcuga ucgccccggc cuacuccauc      600

agaugcaucg gggucagcaa cagagacuuc gucgaaggua ugucuggagg aaccugggua      660

gacauaguac uagaacacgg aggaugcguc acggucaugg cucaggacaa acccaccgua      720

gauauagaac uggucaccac aacgguaagc aauauggccg aaguaagauc cuacugcuac      780

gaggccagca uaucagacau ggcauccgac ucacgcugcc caacccaagg agaagcauac      840

cucgacaaac aaagcgauac gcaauacgug ugcaaaagaa cccuaguaga ccgaggaugg      900

ggaaacggcu gcggccuguu cggcaaaggg ucacugguaa ccugugcgaa auucgcgugc      960

ucaaagaaaa ugacagggaa auccauccaa cccgaaaacc ucgaguaccg aauaaugcua     1020

agcguccacg ggucacaaca cuccggaaug aucgucaacg acacaggaca cgaaacagac     1080

gaaaacagag caaaaguaga aaucacaccg aacagccccc gugcugaggc aacacucggg     1140

ggauucgggu cccuaggacu cgacugcgaa ccacgaacgg gacuagacuu cagcgaccua     1200

uacuaucuca cgaugaacaa caaacacugg cuagugcaca aagaaugguu ccacgacauc     1260

ccccuacccu ggcacgcagg agccgacacu ggcacacccc acuggaacaa caaagaagca     1320

cugguagagu ucaaagacgc acacgccaag agacagaccg uagucguacu cggaagccaa     1380

gagggggcgg ugcacacagc acucgcagga gcgcucgaag cagagaugga cggggccaaa     1440

ggccgacuau ccagcggaca ccucaaaugc cgacuaaaaa uggacaaacu cagacuaaaa     1500

ggagucuccu acagccuaug cacagcagcc uucaccuuca ccaagauacc cgcagaaaca     1560

cuccacggca ccgucaccgu cgaaguacaa uacgccggga ccgauggacc augcaaaguc     1620

ccagcgcaaa uggcagugga caugcaaacc cuaaccccag ucggacgucu gaucaccgca     1680

aaucccguca ucacagaauc caccgaaaau agcaaaauga ugcuggaacu ggacccaccc     1740

uucggcgaca guuacauagu caucggaguc ggagaaaaga aaauaacaca ccauuggcac     1800

agguccggga gcaccauagg caaagcauuc gaagcaacag uacgaggagc caaaagaaug     1860

gccgucuugg gagacacugc augggacuuc ggcuccguag gaggagcacu caacagccuc     1920

ggaaaaggaa uccaccagau auucggcgca gcauucaaau cccucuucgg aggcaugagc     1980

ugguucuccc aaauacuuau aggcacgcuc cuaauguggc uaggccucaa uaccaaaaac     2040

ggaagcaucu cucuaaugug ccucgcgcua ggaggcgucc uaauauuccu aucaacagca     2100

guuucagcag acugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  9434
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9434
gggagaaagc uuaccaugaa agaaaagaaa cgccgagggg cggacacaag cguggguauc       60

gucggccuac ucuuaaccac cgcgauggcc gcggagguca cccgacgagg cuccgcguac      120

uacauguacu uagaccgaaa ugacgccggc gaagccauau cauuccccac cacgcucggc      180

augaacaaau gcuacaucca aauaauggac cucggccaca ugugcgacgc caccaugagu      240

uaugaaugcc ccaugcucga cgaaggggua gagccagacg acguagacug uuggugcaac      300

acaaccagca caugggucgu guacggaacc ugccaccaca agaaaggcga agcccggcgc      360

agccgccggg cagucacacu ccccagccau agcacccgca aacuacagac ccgcucgcaa      420

accuggcucg aaucaagaga auacaccaaa caccucaucc gcgucgaaaa cuggauauuu      480

cgaaaccccg gauucgcccu ggcagcagca gcaauagccu ggcuucucgg cuccagcaca      540

agccagaaag ucaucuaccu cguaaugauc cuauuaauag ccccagcuua cuccauccgg      600

uguauagggg ucuccaacag agacuucguc gaaggcaugu ccgguggaac cuggguagac      660

aucguacucg aacacggagg augcgucacc gucauggccc aagacaaacc caccguggac      720

aucgaacucg ucacaaccac agucucgaac auggccgaag ucagguccua cugcuacgaa      780

gcgucaauau cagacauggc auccgacagc cgaugcccaa cccagggaga agcauaccuu      840

gacaaacaau cggacaccca auacgucugc aagcgcacac ucguggacag aggauggggc      900

aacggaugcg gacuauuugg caaagggagc cuaguaaccu gcgccaaauu cgcgugcagc      960

aagaaaauga cuggcaaaag uauccaacca gagaacuuag aauaucgaau caugcucagc     1020

guccacggaa gccaacacuc cggcaugauc gucaacgaca caggccacga gacagacgaa     1080

aaccgugcca aaguagaaau aacaccaaau agcccccgag cagaggcaac ccucggaggc     1140

uucgggagcc uaggacucga cugcgagccg aggacgggac uagauuucag cgaccuauac     1200

uaccuaacaa ugaacaacaa acauuggcuu guccacaaag aaugguucca cgacauccca     1260

cuccccuggc acgccggagc cgacacuggc acaccucacu ggaacaacaa agaagcacuc     1320

guagaguuca aggacgccca cgcuaaacgc caaacaguug uaguacucgg cagccaagaa     1380

ggagccgucc acacagcacu cgcaggcgcc cucgaagccg aaauggaugg agccaagggc     1440

cgacuaucgu caggacaccu caagugccgg cucaagaugg acaaacucag acugaaagga     1500

guaagcuaca gccuaugcac cgccgccuuc accuucacca agauccccgc agaaacacuc     1560

cacggaacag ucaccgucga aguacaguac gccggaaccg acggccccug caaagucccc     1620

gcacaaaugg ccguggacau gcagacccua acaccaguag gacgccucau aacagcaaau     1680

ccaguaauaa ccgagucaac cgaaaacagc aaaaugaugc uagaacuaga uccacccuuc     1740

ggcgacuccu acauaguaau cggcguaggc gagaagaaaa uaacacauca cuggcacaga     1800

ucagguucca caaucgggaa agcauucgaa gccacagucc gcggagccaa aagaauggcg     1860

guacuaggcg acacagccug ggacuucgga aguguaggug gcgcgcucaa cucccuaggg     1920

aagggaauac accaaaucuu cggcgccgca uucaaaagcc uauucggcgg aaugagcugg     1980

uucagccaaa uucucauagg cacccuccua auguggcuug gacucaauac aaagaacggg     2040

agcauauccc uaaugugccu cgcccuaggc ggcguccuaa uauuucugag cacagcaguc     2100

agcgcagacu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9435
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9435
gggagaaagc uuaccaugga aaagaagagg cgaggagccg acaccagcgu agggauagua       60

ggccuacucc ucacaacagc uauggcagca gaagucacac gacgcggcag ugcauacuac      120

auguaccucg accgaaacga ugccggcgag gccaucagcu uccccaccac acuaggcaug      180

aacaaaugcu acauacaaau cauggaccuc ggacacaugu gcgacgcaac aauguccuac      240

gaaugcccaa ugcucgacga ggggguagaa cccgacgacg uagacugcug gugcaacacg      300

accuccacau gggugguuua cggcacaugc caccacaaga aaggcgaagc ccgacgaagc      360

cgacgugcag ugacacuccc aucgcacucc acacgaaaac uacaaacacg aucucaaaca      420

uggcuagaau cccgcgaaua cacaaaacac cuaauacggg uggaaaacug gauauucaga      480

aaccccggcu ucgcccuagc agcagccgca auagcauggc ucuuagggag cuccaccuca      540

caaaaaguca ucuaccucgu aaugaucuua cuaauagcgc cggccuacag cauacgcugc      600

auagggguaa gcaaccgaga cuucgucgaa ggaaugagcg gaggcaccug gguagauauc      660

guacuagagc acggcggcug cgucacugua auggcacaag acaaaccuac aguagacauc      720

gaauugguaa caacaacagu cagcaacaug gccgaaguca gaucauacug cuacgaagcg      780

agcauaagcg acauggcauc ugacagccgc ugccccaccc agggcgaagc cuaccucgac      840

aaacagucag acacccaaua cguaugcaaa agaacacucg uagaccgagg auggggcaau      900

ggaugcggac ucuucgggaa aggaagccua gucacaugcg ccaaauuugc augcucaaag      960

aaaaugacag gaaaaucgau ccaaccagaa aacuuagaau aucgaaucau gcugagcgua     1020

cacggcagcc aacacagcgg caugauaguc aacgacacug gacacgaaac cgacgaaaac     1080

cgggcaaaag ucgaaaucac cccaaacuca ccaagggccg aagcaacacu cggcggcuuu     1140

ggaagccuag gccucgacug cgaaccccgc accggacuag acuucucaga ccuauacuac     1200

cuaaccauga acaacaaaca cuggcuaguu cacaaggaau gguuccacga cauaccccuc     1260

ccauggcacg ccggcgcaga cacaggaacg ccccacugga acaacaaaga agcacucgua     1320

gaguucaagg acgcccacgc caagcggcag acaguagucg ugcucggauc acaagaagga     1380

gccguccaca cagcccucgc cggagcccuu gaagcggaaa uggacggcgc aaaaggacgg     1440

cugucuagug gacaccuaaa augucggcug aaaauggaca aacugcgucu aaaaggugua     1500

agcuacagcc uaugcacagc agcguucacc uucaccaaaa ucccggcaga aacgcuccac     1560

ggaaccguca caguagaagu acaauacgca gggacagacg gcccaugcaa aguacccgcc     1620

caaauggccg uagacaugca aacacuaaca ccagucggaa gacucauaac agccaacccc     1680

guaaucacag aaagcaccga gaacuccaaa augaugcugg aacucgaucc ucccuucggc     1740

gacagcuaua uagucauagg ugucggugag aagaaaauaa cacaccacug gcaccgcagc     1800

ggcagcacaa ucggaaaagc cuucgaagca accguucggg gagcgaaacg aauggccguc     1860

uuaggcgaca cagcauggga cuucggcucu guaggcggag cacugaacag ccuaggcaaa     1920

ggaauccacc agauauucgg cgcagccuuc aaaucacucu ucggcgggau gucuugguuc     1980

ucacaaauac ucauaggaac acuccucaug uggcuaggcu uaaacaccaa aaacggaucg     2040

aucucgcuga ugugccuagc acucggcgga gugcugauau uccuaucgac cgcaguaucg     2100

gccgacugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  9436
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9436
gggagaaagc uuaccaugaa gaaacgccgc ggcgccgaca ccagcguagg aaucgucgga       60

cuacuguuga caaccgcaau ggccgcggaa gugacacgaa ggggaagcgc cuacuacaug      120

uaccucgaca gaaacgacgc cggagaagcc auauccuucc cgaccacacu aggcaugaac      180

aaaugcuaca uacaaauaau ggacuuaggc cauaugugcg acgcaacgau gucauacgaa      240

ugcccuaugc uagacgaagg aguggaaccc gacgacgucg acugcuggug caacacgaca      300

ucgaccuggg uagucuacgg cacuugccac cacaagaaag gagaagcacg acgaagccga      360

cgggcaguca cgcuacccag ccacuccacc cgaaaacucc aaacucgcag ccaaaccugg      420

cucgaaagcc gcgaauacac caagcaccua auacgagucg aaaacuggau auuccggaac      480

ccaggcuucg cccucgcagc agcagcaaua gccuggcucc uuggaagcuc cacaucccaa      540

aaaguaauau accuagucau gauacuccua aucgcccccg cauacagcau ccgauguauc      600

ggaguaucca accgcgacuu cgucgagggc augagcggcg gcaccugggu agacauaguc      660

cucgaacacg gaggcugcgu cacaguaaug gcccaagaca aacccacagu agacaucgag      720

cuggucacca caacgguaag caacauggcc gaaguccgau ccuacugcua cgaagcaagc      780

auaucagaca uggcaucaga cagcagaugc cccacacaag gcgaagccua ccuagacaaa      840

caaagcgaca cacaauacgu augcaaacgg acguuggucg accggggcug gggcaacgga      900

ugcggucucu ucggaaaagg cagccucgua accugcgcca aauucgcgug cucaaagaaa      960

augacgggca aaucaaucca accggaaaau cuagaguacc ggauaaugcu aagcguccac     1020

ggaucacagc acuccggcau gaucgucaac gacaccggac acgaaacaga cgaaaaccgu     1080

gcaaaagugg agaucacacc aaacagcccc cgcgccgaag ccacacuagg cggauucgga     1140

ucccugggac uagacugcga accaaggaca ggacuagacu ucuccgaccu cuacuaccuc     1200

acaaugaaua acaaacacug gcuaguacac aaagaauggu uccacgacau cccacucccc     1260

uggcacgccg gagcagacac aggcacacca cacuggaaca acaaagaagc acucgucgag     1320

uucaaagacg cacaugccaa acgccaaacc gucguagugc uaggauccca agaaggagca     1380

guccacaccg cgcucgccgg agcccuggaa gccgaaaugg acggagcaaa agggcgccug     1440

aguuccggcc accuaaagug ccgccuaaag auggacaaac uaagacucaa aggcgucucc     1500

uacagccucu gcaccgcagc guucaccuuc acuaaaaucc cggccgaaac acugcacgga     1560

acaguaaccg uggaggugca guacgcaggc acagacggac ccugcaaagu gccggcccaa     1620

auggcagucg auaugcaaac acuaacgccc guaggacgac ucaucacagc caaccccgua     1680

aucacagagu ccacagaaaa cucaaaaaug augcuagaac uagauccccc auucggagac     1740

agcuacauag ucaucggcgu cggugaaaag aaaauaacac accauuggca cagaagcggc     1800

ucaaccaucg gaaaagccuu cgaagccacg guccgagggg caaaacgcau ggccguccua     1860

ggggacacgg caugggacuu cgggagcgua gggggagccc ucaacucccu gggcaaggga     1920

auacaccaaa uauucggcgc cgcuuucaaa agccucuucg gcgggaugag cugguucagc     1980

caaauccuca ucggaacacu ucucaugugg cucggucuaa acaccaaaaa cggaagcaua     2040

ucgcugaugu gccucgcgcu uggcggcguc cuuauauucc uaucaaccgc cguuuccgcc     2100

gacugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  9437
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9437
gggagaaagc uuaccaugaa gcgccgcgga gccgacacaa gcguaggaau ugucggccuc       60

cuucuuacaa cagcgauggc ggccgaagua acccgaagag gcuccgccua cuauauguac      120

cucgacagga acgacgccgg cgaagcaauc ucauucccca caacccuggg aaugaacaaa      180

ugcuacaucc agauuaugga ccuaggccac augugcgaug ccaccauguc guacgaaugc      240

cccaugcuag acgaaggagu agaaccagac gacguagacu gcuggugcaa cacaaccagc      300

acaugggucg uauacggaac augccaccac aaaaagggcg aagcgcgaag aagccgccgc      360

gcagugaccc ucccaagcca cagcacacga aaacuccaaa cacgcagcca aacauggcuc      420

gaaagccggg aauacaccaa acaccucauc agggucgaaa acuggaucuu ccgaaacccc      480

ggcuucgcac ucgcugcagc agcaauagcc uggcuucucg gauccagcac cucccaaaaa      540

gucauauacc uagucaugau acuccuaaua gcccccgcau acucuauacg cugcauagga      600

gugaguaacc gagacuucgu ggaagggaug uccggcggaa caugggucga caucguccua      660

gaacacggug gcugcguaac aguaauggca caagacaaac ccacagugga caucgagcuu      720

guaacaacca caguauccaa cauggcagaa gucagauccu acugcuacga agccuccaua      780

ucugacaugg ccucagacuc acgaugccca acccaaggag aagcauaccu agacaaacaa      840

agcgacacac aauauguaug caaaagaaca uuagucgacc gcggaugggg aaacggaugc      900

ggacuuuucg gaaaagguuc ccuagugacu ugcgccaaau ucgccuguuc gaagaaaaug      960

accggcaagu ccauccaacc ggaaaaccug gaauaccgaa ucaugcuauc uguccacggc     1020

ucgcaacacu caggcaugau cguaaacgac acgggccacg aaacagacga aaacagagcc     1080

aaagucgaga uaacccccaa cuccccucgc gccgaagcca cgcucggcgg auucggaagc     1140

cugggccucg acugugaacc gcgaacagga cuagacuuca gcgaccucua cuaccuaacc     1200

augaacaaca agcacuggcu aguccacaaa gaaugguucc acgacauccc acucccaugg     1260

cacgcaggag ccgacaccgg aacaccacac uggaacaaca aggaagcccu cgucgaguuc     1320

aaagacgcgc acgccaaacg ccaaacagug gucguccuag gaucccaaga aggcgccguc     1380

cacaccgcac uagcgggagc ccucgaagcc gaaauggacg gcgcaaaagg aagacucagc     1440

ucgggccacc uaaaaugcag acuaaaaaug gauaagcucc gacugaaagg aguaucuuac     1500

ucccuaugca cagcagcauu caccuuuacu aaaauccccg cagaaacacu gcacggaaca     1560

guaaccgucg agguacaaua cgcaggcacc gacggacccu gcaaaguacc ggcccaaaug     1620

gccgucgaca ugcaaacccu cacaccaguc ggaagacuca ucacggcaaa ccccguaauc     1680

accgaaucca ccgaaaacuc uaaaaugaug cuggagcuag acccacccuu cggcgacagc     1740

uacaucguca ucggaguggg agagaagaag aucacccacc acuggcaccg aagcggcagc     1800

accaucggca aagcauucga ggcgacuguc cgcggagcga aaagaauggc cguucucggg     1860

gacaccgcau gggacuucgg guccgucggc ggcgccuuaa acagucucgg aaaaggcaua     1920

caccaaauau ucggcgccgc guucaaaagc cuguucggag gcauguccug guucucgcaa     1980

auacuaaucg gcacccuccu uauguggcuc gggcuaaaca ccaaaaacgg aagcauaagc     2040

cuaaugugcc uagcccuagg cggcgugcuc aucuuccuua gcacagccgu aagcgcggac     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  9438
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9438
gggagaaagc uuaccaugag acggggcgca gacacaagcg ucggaaucgu cggacuccua       60

cuuacgaccg caauggcggc ugaagucaca cgccgaggau ccgcauacua cauguaccuc      120

gaccguaacg acgcaggcga agccauauca uucccuacaa cgcugggaau gaacaaaugc      180

uacauccaga uaauggaccu aggccacaug ugcgacgcaa ccauguccua cgaaugccca      240

augcucgacg aaggggucga acccgacgac guagacugcu ggugcaacac aacgagcaca      300

uggguagugu acggaacaug ccaccacaaa aagggggaag cccgacgauc caggagagca      360

gugacacucc caagccacag cacaagaaaa cuacagacaa gaucacaaac cuggcuugaa      420

agcagagaau acacaaagca ccuaauacga guugaaaacu ggauauucag aaaccccggc      480

uucgcacucg cagcagccgc cauagccugg cuccugggca guucaacgag ccaaaaaguc      540

auauaccuag uaaugauacu gcuaaucgcc ccagcauacu caauacgcug caucggcgua      600

uccaaccgag acuucguaga aggaauguca ggaggaaccu ggguagacau uguacucgaa      660

cacggaggau gcguaacagu cauggcccag gacaaaccca cggucgacau cgaacucgua      720

acaacaaccg uaucaaacau ggcagaagua cgauccuacu gcuacgaagc aagcaucucu      780

gacauggccu cagacucacg augccccacc cagggcgaag ccuaccuaga caaacagucg      840

gacacacaau acgucugcaa acgaacacug gucgaccgcg guuggggcaa cggcugcggc      900

cuguucggaa aagggagccu agugaccugc gccaaauucg ccugcagcaa gaaaaugacc      960

ggcaaaucga uacaacccga aaaccuugag uacagaauca ugcuauccgu ccacggcagc     1020

caacauuccg gcaugauagu caacgacacc ggccacgaaa cagacgaaaa cagagcaaaa     1080

gucgaaauca cgcccaacuc cccacgggcg gaagccacac ugggaggauu cggcagccuc     1140

ggccuagauu gcgaaccacg cacaggguua gacuucuccg accucuacua ccuuacaaug     1200

aacaacaaac auuggcucgu gcacaaagaa ugguuccaug acaucccacu ccccuggcau     1260

gcaggcgccg acacggggac accacacugg aauaacaagg aggcgcuugu ggaguucaag     1320

gaugcgcacg ccaaacgaca aacaguaguu guccuaggcu cacaagaagg cgcgguccac     1380

acagcccucg ccggggcacu agaagccgaa auggacggcg ccaaagggcg acucagcagc     1440

ggccaccuca aaugccgacu aaaaauggac aaacugcgcc uaaaaggggu cagcuacagc     1500

cucugcaccg ccgcauucac cuucacaaaa auaccagccg aaacccuaca cggcacaguc     1560

acaguagaag uacaauacgc cggcacagac ggacccugca aagucccagc ccaaauggcg     1620

gucgacaugc aaacccucac gccaguaggc cgacuaauca cagcaaaccc agucaucacc     1680

gagagcacag aaaacagcaa aaugaugcua gaacuggacc cgccauuugg agauaguuac     1740

aucguaauug gcguggggga aaagaaaaua acacaccacu ggcacagaag cggcuccaca     1800

aucggaaaag cauucgaagc uaccguccga ggagcgaaac gaauggccgu acucggcgac     1860

acagccuggg acuucggcuc aguaggcgga gcccugaacu cccucggcaa aggaauacac     1920

caaaucuucg gggccgcguu caaaucccug uucggaggaa ugagcugguu cucacagaua     1980

cucauaggca cacuacuaau guggcuaggc cuaaacacaa agaacggcag cauuagcuug     2040

augugccuag ccuugggagg cguacuaaua uuccuaagca cagccguauc agccgacuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  9439
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9439
gggagaaagc uuaccaugcg aggagccgau acuucggucg gcauagucgg ccuacuccua       60

accacggcca uggcugcaga agucacaaga cggggcucag ccuacuacau guaccucgac      120

agaaacgaug caggagaagc aaucucguuc cccacaacac ugggcaugaa caaaugcuau      180

auacaaauca uggaucuugg ccacaugugc gacgcaacca ugagcuacga gugcccaaug      240

cucgacgaag gcgucgaacc cgaugacgug gacugcuggu gcaacacaac cagcacaugg      300

gucguauacg gaaccugcca ccacaagaaa ggcgaagccc gccgaucacg gcgcgcagug      360

acccuccccu cacacuccac ccgaaaacua caaacaagaa gccaaaccug gcucgaauca      420

cgcgaguaca ccaaacaccu aaucagagua gaaaacugga uauuccgcaa cccaggcuuc      480

gcacuggccg ccgcggcaau agcauggcuc cuuggcucaa gcacaucaca aaaaguaauu      540

uaccucguaa ugauacuacu caucgccccc gccuauagca uccgcugcau cggcgucuca      600

aaucgcgacu ucguagaagg caugagcggc ggaacauggg uagacaucgu ccucgaacac      660

ggagguugcg ucacggucau ggcccaagac aaacccacag uagacaucga gcuagucacc      720

acgacagucu cuaacauggc ugaaguacga agcuauugcu acgaagcgag uaucuccgac      780

auggcaucag acucacguug ccccacccaa ggagaggccu accucgacaa acaauccgac      840

acacaauacg ucugcaaacg cacccucgua gacagaggau gggggaacgg cugcggacug      900

uucggcaaag gaagccucgu aacaugcgca aaauucgcau gcucaaagaa aaugaccgga      960

aaaucaauac aaccagaaaa ccucgaauac cgcauaaugc uauccguaca cggcucccaa     1020

cacuccggaa ugauaguaaa cgacaccggc cacgaaaccg acgagaaccg agcaaaaguc     1080

gagaucaccc cuaacucccc acgcgcagaa gcaacccuag gcggcuucgg cucccucgga     1140

cuggacugcg aacccagaac cggacuggac uucucagacc ucuacuaccu cacaaugaac     1200

aacaaacacu ggcucguuca caaggagugg uuccacgaca ucccccuccc cuggcacgca     1260

ggcgcggaca cagguacgcc acacuggaac aauaaggagg cacucgucga guucaaagac     1320

gcacacgcaa aacgccaaac ugucgucgua cugggcuccc aagaaggagc cguacacaca     1380

gcccucgccg gggcacucga agccgaaaug gacggcgcca agggacgacu aagcuccggc     1440

caccucaaau gccgacuaaa aauggacaag cuacggcuca aaggcgucuc guacagccuc     1500

ugcacagcgg ccuucaccuu caccaaaauc cccgccgaaa cucuacacgg cacagucacc     1560

guagaagucc aguacgcagg aaccgacggc cccugcaagg ugccagcaca aauggccgua     1620

gauaugcaaa cccuuacccc cguaggccgc cucauuaccg ccaaccccgu gauaaccgag     1680

agcaccgaaa acagcaaaau gaugcuagaa cucgaucccc cauucggcga caguuacauc     1740

gucaucggcg ucggagagaa gaaaaucaca caccacuggc acagauccgg uucaacaaua     1800

ggaaaagccu ucgaagcaac cguccgcgga gccaaacgca uggcaguacu cggcgacacc     1860

gcaugggacu ucggauccgu cggaggagcc cuaaacuccc ucggcaaggg aauacaccaa     1920

aucuucggcg ccgccuucaa aagccucuuc gguggaaugu caugguucag ccaaauccug     1980

aucgggacac uucuaaugug gcuuggccua aacaccaaaa acggcagcau cagucucaug     2040

ugucuagccc ucggcggagu acucauauuc cuaucaacag caguauccgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  9440
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9440
gggagaaagc uuaccauggg ggcggauacc uccguaggaa uagucggacu acuacucacu       60

accgcaaugg ccgcagaggu aacacgacgc ggcagcgccu acuacaugua ccuagaccga      120

aacgacgcag gagaagcaau cagcuucccc acgacacugg gaaugaacaa gugcuacaua      180

cagaucaugg accucggcca caugugcgac gcaaccauga gcuacgaaug cccaaugcua      240

gacgaaggag uagaacccga cgacguagac uguuggugua acaccacaag caccuggguc      300

gucuacggaa caugccacca caagaaggga gaggcccgcc gcagcagacg agccguaaca      360

cuacccucgc acagcaccag aaaacuacag accagaagcc aaacauggcu agaaagcaga      420

gaauacacaa aacaccuaau ccggguagaa aacuggauau uuaggaaccc gggauucgca      480

cuagcagccg cagcaauagc auggcuacua gggagcucaa ccucccaaaa aguaauauac      540

cuagucauga uccuacucau cgcgccagca uacuccaucc ggugcauagg aguaucaaac      600

agagauuucg uagaaggcau gucagggggc accuggguag acauaguccu cgaacacgga      660

gggugcguaa cggugauggc acaagauaaa ccaaccguag acaucgagcu aguaacaaca      720

acagucagca acauggccga aguaaggucg uacugcuacg aagccaguau cucagacaug      780

gccagcgacu cacgaugccc aacccaagga gaagccuacc uagacaaaca guccgacaca      840

caguacguau gcaaacgcac gcucgucgac cgaggcuggg gaaacggcug cggccuguuc      900

ggaaaaggga gccuggucac augcgcaaaa uucgccugcu ccaagaaaau gaccggaaaa      960

uccauacaac ccgaaaaccu agaauaccgg aucaugcuca guguccacgg cagucagcau     1020

agcggcauga ucguaaacga caccggacac gaaacagaug aaaaccgagc aaaagucgaa     1080

auaaccccaa acucgccccg agcagaagcc acauuaggcg gcuucggauc ccuaggacua     1140

gacugcgaac cccgaaccgg acucgacuuc ucagaccugu auuaucucac gaugaacaac     1200

aaacacuggc ugguacacaa ggaaugguuc cacgacauac cacuaccaug gcacgcugga     1260

gcugacaccg gaacaccaca cuggaacaac aaagaggccc uaguagaguu caaagaugca     1320

caugccaaac gccaaaccgu gguaguccua gggucccaag aaggagccgu gcacaccgca     1380

cucgccggcg cacuggaagc agaaauggac ggagccaaag ggcgacuaag cuccggccac     1440

cuaaaaugca gacucaaaau ggacaaauua cgacuuaaag gcguaagcua uucacugugc     1500

acggcggcau ucacauucac caaaauaccc gccgaaaccc uacacggaac agucaccgua     1560

gaaguccaau acgccgggac agacggaccg ugcaaagucc cagcccaaau ggccguagac     1620

augcaaacac ucacccccgu aggaagacuc auaaccgcaa acccggucau caccgaauca     1680

accgaaaaca gcaaaaugau gcucgaacua gacccgcccu ucggggacag cuauauagug     1740

auaggcguag gagaaaagaa aauaacgcac cacuggcacc gaagcggcag cacaauaggg     1800

aaagccuucg aagccacagu acgaggagcg aaaagaaugg caguccuggg agacaccgcg     1860

ugggacuucg gaucgguagg aggcgcacua aacagccugg gaaaaggaau acaccaaauc     1920

uucggcgccg ccuucaaauc acucuucggc ggaaugucau gguucuccca gauacuaauc     1980

gggacccucc ugauguggcu aggucuaaac accaaaaaug gcucaaucuc acuaaugugc     2040

cuagcgcuag gaggcguccu caucuuccug uccacagccg uaucggccga cugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9441
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9441
gggagaaagc uuaccauggc agacaccagu guaggcaucg uaggacuccu acuaaccaca       60

gcaauggccg cugaagucac acgacgcgga agcgcauacu acauguaccu agaccguaac      120

gacgccggcg aagcaaucuc guuccccacg acccucggaa ugaacaaaug cuacauucaa      180

auuauggacc uagggcacau gugcgacgcg acaaugagcu acgaaugccc caugcucgac      240

gaaggcgugg aaccagacga cgucgacugc uggugcaaca caacaagcac augggugguc      300

uauggaaccu gccaucacaa gaaaggcgaa gcacgaagaa gccgccgggc aguaacacuu      360

ccgagucaca gcacccgaaa acuccaaaca cgcagccaaa cauggcuaga aagccgcgaa      420

uauacgaaac accuaaucag aguagaaaac uggauauucc gaaacccugg cuuugcacuc      480

gccgcagcag ccauagcaug gcuccugggc agcagcacaa gccaaaaagu caucuaccua      540

gucaugauac uccuuaucgc ccccgcauac ucaauccgcu gcaucggcgu cucgaacagg      600

gacuucgugg agggcaugag ugggggaacc uggguagaca ucguccuaga acacggcggc      660

ugcgucacgg uaauggcaca ggacaaacca acaguagaua uagagcuagu aaccacuaca      720

guauccaaca uggcagaagu ccgcagcuac ugcuacgaag caucgauauc ggacauggcc      780

agcgauucca gaugcccaac ccaaggggag gccuaccucg acaaacaaag cgacacccag      840

uacguaugca aaagaacacu cguggacaga ggguggggca acggcugcgg acuauucggc      900

aagggaucac ugguaaccug cgccaaauuc gccugcucca agaaaaugac cggcaaaagc      960

auacagccag aaaaccuaga auacagaauc augcucuccg uacacggcuc gcaacacagc     1020

ggaaugaucg uaaacgacac aggacacgaa acagacgaaa accgcgcaaa agucgaaauc     1080

accccgaacu ccccgcgcgc agaagcaaca cuaggcggcu ucggaagccu cggacuagac     1140

ugcgaaccuc gaaccggccu cgacuucagu gaccucuacu accucacaau gaacaacaaa     1200

cacuggcuag uacacaaaga augguuccac gacauccccc uacccuggca cgcgggcgca     1260

gacacaggca caccacacug gaacaacaaa gaagcccugg uggaguucaa ggacgcccac     1320

gcaaaacgac aaaccgucgu cguacuuggg agucaagaag gcgcaguaca cacagcacuc     1380

gccggagcac ucgaagccga aauggacggu gccaaaggac gccugagcag cggccaccuc     1440

aagugcagac uaaagaugga caaacuaaga cucaaaggag ugagcuacag ccuuugcacc     1500

gcagccuuca ccuucacaaa aauaccggcc gaaacccugc acggaacggu caccguagaa     1560

guccaguacg ccggaacuga cggacccugc aaggucccag cacaaauggc gguagacaug     1620

caaacccuaa caccaguagg gagauuaaua accgccaacc ccguaaucac agaaagcacg     1680

gaaaacagca agaugaugcu agaacucgac ccgcccuucg gggacagcua cauaguaaua     1740

ggagucggag aaaagaagau cacccaccac uggcaccgaa gcgggagcac caucgguaaa     1800

gcguucgaag caacaguacg cggcgccaaa cgcauggccg uacucgguga caccgccugg     1860

gacuucggcu cggucggcgg ggcccucaau agccuuggca aaggcauuca ccaaaucuuc     1920

ggcgccgcau ucaaaagccu auucggcgga augagcuggu ucagccagau acucauaggu     1980

acacuucuaa uguggcucgg gcucaacacc aaaaacggcu caaucagccu aaugugccug     2040

gcccucggag gagugcuaau cuuccuaagc acagccguca gcgccgacug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  9442
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9442
gggagaaagc uuaccaugga cacaagcguc ggcaucguag gacuacuacu cacaacagcc       60

auggcagcug aagucaccag acgcggcucc gcauacuaca uguacuuaga ccgcaacgac      120

gccggcgaag ccaucucauu cccaacaaca cugggaauga acaaaugcua cauccaaauc      180

auggaccucg gacacaugug cgacgcaacg augucauaug agugcccuau gcuggacgaa      240

ggagucgaac cagacgacgu agacugcugg ugcaacacca cgagcacgug ggucguauac      300

ggcacuugcc accacaagaa aggggaagcc cgccgcucac gccgagccgu aacccuacca      360

ucccacucaa cccggaaacu acaaacacgc ucccaaacau ggcuagaaag cagagaguac      420

acaaaacacc ugauacgcgu cgaaaacugg auauuccgaa accccggauu cgcccucgcc      480

gcugccgcca uagccuggcu ccugggcagc uccaccucac agaaagucau cuaucucgua      540

augauccuac ucaucgcccc cgcauacagc auacgaugca uaggcgucuc aaaccgcgac      600

uucgucgaag gaauguccgg aggcaccugg guugauaucg uccuagaaca cgguggaugc      660

guaaccguca uggcacaaga uaaacccacc gucgacaucg aacucguaac aaccacaguc      720

ucgaacaugg ccgaaguacg auccuacugc uacgaagcca gcaucucgga cauggccucc      780

gacagccgcu gccccaccca aggcgaagca uaccucgaca aacaaagcga cacucaauac      840

guaugcaagc ggacacuugu agaccgaggc ugggggaacg gcugcggacu cuucggcaaa      900

ggcagccuug uaacgugcgc aaaguucgcc ugcucaaaga aaaugaccgg aaaauccaua      960

caacccgaaa accuugaaua ccgaauaaug cuaagcgucc acggaucgca acauuccgga     1020

augaucguca acgauaccgg ccacgaaacc gacgaaaacc gagcaaaagu cgaaaucaca     1080

cccaacagcc cccgcgcaga agccacccua gggggcuucg gaagccucgg acuagacugc     1140

gaaccgcgca caggccucga cuucagcgac cuguacuacc ucaccaugaa caauaaacac     1200

uggcuagucc auaaagagug guuccacgau auccccuuac cauggcacgc aggagcagac     1260

accggcaccc cacacuggaa caacaaagag gcacucgucg aguucaaaga cgcacacgcc     1320

aaacgccaaa ccgucgucgu acucggcuca caagaaggcg ccguccauac agcccuggcg     1380

ggagcccuag aagcagaaau ggacggagcc aaaggcagac uaagcucggg ccaccucaaa     1440

ugccgccuaa aaauggacaa acucagacuc aaaggaguaa gcuacucuuu augcaccgcc     1500

gcguucacau ucacaaaaau ucccgcagaa acccuccacg gaacaguaac cguagaagua     1560

caauacgccg gcaccgacgg ccccugcaaa gucccggccc agauggccgu ugacaugcaa     1620

acccuaacac ccgucggcag acuaauaacc gccaacccag uaaucaccga auccacggaa     1680

aacucaaaaa ugauguuaga gcuagaucca ccguucggag acagcuacau cgugaucggc     1740

gucggcgaaa agaaaauaac ccaccacugg caccgcagcg gaagcaccau aggcaaagcc     1800

uucgaagcca ccguccgagg ggcgaaacgc auggcggucc uaggagacac agcuugggac     1860

uucggcagcg uaggcggagc gcuaaacuca cucggcaaag gcauccacca aaucuucggc     1920

gcggcauuca aaucguuguu cggaggaaug ucaugguucu cacagauacu caucggcaca     1980

cuccugaugu ggcuaggccu caacaccaaa aauggcucaa uaagccuaau gugccuagca     2040

cucggcggcg uccuaaucuu ccugagcaca gccgucagcg cugacugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  9443
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9443
gggagaaagc uuaccaugac aagcguagga auugucggac uccuacuaac aaccgcgaug       60

gcagcagaag uaacgcgacg cggcucagcc uacuacaugu accuagaccg aaacgacgcc      120

ggagaagcca ucucauuccc gaccacacuc gggaugaaua aauguuacau acaaauuaug      180

gaccuaggcc acaugugcga cgcaaccaug ucguacgaau gcccgaugcu cgacgaggga      240

gucgaaccgg acgacguaga cugcuggugc aacaccacgu ccacaugggu cgucuacggg      300

accugccacc acaagaaagg agaagcacga cgaagccgac gggcgguuac ccuaccaagc      360

cacagcacac gcaaacucca aacacgcagu caaaccuggc ucgaaucccg agaauacacc      420

aaacaccuaa uacgcgucga gaacuggauc uucaggaauc caggauucgc ccucgcagca      480

gcagccaucg cauggcuccu gggcucgagc acuagccaaa aaguaauaua cuuaguaaug      540

auccuacuga ucgcccccgc auacucaaua cgaugcaucg gcguaagcaa ccgcgacuuc      600

gucgaaggca ugucaggagg aacaugggua gacauagucc ucgaacacgg gggcugcguc      660

acgguaaugg cacaggacaa accaacaguc gacauagagc uagugaccac cacagucagu      720

aacauggcgg aaguccgauc guacugcuac gaagccagca uaucagacau ggccucagac      780

ucacgcugcc ccacacaggg agaggccuac cucgacaagc aaagcgacac acaauauguc      840

ugcaaaagaa cauuagucga ucgcgggugg ggcaacgggu gcggccucuu cggcaaaggc      900

agucucguaa caugcgcaaa auuugcaugc uccaagaaaa ugacaggaaa auccauacaa      960

cccgaaaauc ucgaguacag aaucaugcua uccguccacg gcucccaaca uagcgggaug     1020

auagugaacg acacgggcca cgaaacagac gaaaaccgag ccaaagucga aauaacaccc     1080

aacagucccc gcgcagaagc aacacuaggc ggguucggca gccuaggcuu agacugcgaa     1140

ccacgcacag gcuuagacuu cagcgaccua uacuaccuca ccaugaacaa caaacacugg     1200

cugguccaca aagagugguu ccacgacauc ccacuccccu ggcaugcagg agcugacacg     1260

ggcaccccac acuggaacaa caaagaggcu cucguagagu ucaaagacgc acacgcaaaa     1320

cgacaaacag ucgugguccu aggcucacaa gaaggagcgg uacauaccgc acuagccgga     1380

gcccucgagg ccgaaaugga cggagcgaag gggcgccuaa gcucaggaca ccuaaagugc     1440

cgacuuaaaa uggacaaacu acgauugaaa ggagucuccu acucccucug cacagcagcc     1500

uuuacauuua ccaaaauacc ggccgagacc cugcacggga cuguaacagu ugagguacaa     1560

uacgccggga ccgacggccc augcaaagua ccagcccaaa uggcaguaga caugcagacc     1620

cuaacccccg uagggcgccu gauaaccgcc aauccaguca ucacagaauc aacggaaaac     1680

ucaaaaauga ugcuagaacu agauccccca uucggcgaca gcuacauagu cauaggaguc     1740

ggagaaaaga aaauaaccca ccacuggcac aggucaggcu caacaaucgg aaaagcauuc     1800

gaagccacgg uacgaggcgc gaaaagaaug gcgguccucg gcgacacugc augggacuuc     1860

ggauccguag gcggcgcccu caacucacuc ggaaaaggaa uccaccaaau cuucggcgcc     1920

gcauucaaau cacuguucgg cgggaugucc ugguucagcc aaauacucau cggaacgcua     1980

cuaauguggc uaggccuaaa caccaagaac gggagcaucu cccugaugug ccucgcccua     2040

ggaggaguac ugauauuccu gagcacagcc guaagcgccg acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  9444
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9444
gggagaaagc uuaccaugac cuccgucggc aucguaggac uccuccuaac aaccgccaug       60

gcagccgcag uuacacgacg aggaagcgcc uacuacaugu accuagacag aaacgacgcc      120

ggagaagcca uauccuuccc gacgaccuua ggcaugaaca aaugcuacau acaaauaaug      180

gaccuaggac acaugugcga cgcaacgaug ucauaugaau gccccaugcu cgacgaagga      240

guggaaccug acgacguaga cugcuggugc aacacgacau caaccugggu aguauacgga      300

accugccacc acaagaaagg cgaagcccga cguucccggc gagccguaac ccuccccagc      360

cacuccacac gcaaacuaca gacacgauca caaaccuggc uagagucacg agaauacaca      420

aaacaccuga uacgcguaga gaacuggauc uuccgcaacc ccggauucgc acuagccgcc      480

gcugccaucg cauggcuacu aggcagcucg accucacaaa aaguaauaua ccucguaaug      540

auccuccuaa ucgccccagc cuacuccaua cgcugcaucg gggucucaaa ccgcgacuuu      600

gucgaaggca uguccggagg aaccuggguc gacauaguac ucgaacacgg cggcugugua      660

accguaaugg cacaagacaa gccuacagua gacaucgagc uagucacaac aacaguauca      720

aacauggccg aaguacgcuc cuacugcuac gaagcaagca uaagcgacau ggcaagcgac      780

agccggugcc ccacacaagg cgaagccuau cuugacaaac agagcgacac acaauacguc      840

ugcaaaagga cccucguaga cagaggcugg ggcaacggcu gcggacuauu cggaaaaggg      900

ucacucguca caugcgcaaa auucgccugc aguaagaaga ugaccgggaa aagcauccaa      960

cccgaaaacc uagaauaccg uaucaugcuc agcguacacg gcucacaaca cuccggcaug     1020

auugucaaug acaccgguca cgaaacagac gagaaccgcg caaaagugga aauaaccccc     1080

aacucgccca gggccgaagc gacacucgga ggauucggau cccuuggccu agacugcgaa     1140

cccaggacag gacucgacuu cucagaccuc uacuaccuua ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ucacgacauc ccccuaccau ggcacgcggg agccgacaca     1260

ggcacaccac acuggaacaa caaagaagcc cuagucgaau uuaaggacgc acacgcaaaa     1320

agacaaaccg uaguuguccu aggaucacaa gaaggcgcag uacacacggc ccucgccgga     1380

gcccucgaag ccgaaaugga cggcgccaaa ggccgacuaa gcagcggcca ccucaaaugc     1440

cgccugaaaa uggacaaacu ccgacuaaaa ggugucaguu auucccuaug cacagccgcc     1500

uuuaccuuca caaaaauacc cgcagaaacc cuccacggca ccgucaccgu cgagguccaa     1560

uacgccggca cggacggccc cugcaaggua cccgcacaaa uggccgugga caugcaaacc     1620

cugacccccg ugggcagacu cauaacagca aacccaguca uaacagaaag cacagaaaac     1680

uccaaaauga ugcucgaacu agacccgccc uucggggacu cauacauagu aauaggcgua     1740

ggagagaaga aaaucaccca ccacuggcac cgaucgggau cuaccaucgg caaagcauuc     1800

gaagccaccg ugcgaggcgc caaacgaaug gcaguccucg gcgacaccgc augggacuuu     1860

ggcucugucg gaggagcccu aaacagccuc ggcaaaggca uccaccaaau cuucggugca     1920

gcguucaaaa gccucuucgg cggcaugucc ugguucagcc aaauccugau cgggacccua     1980

cucauguggc uaggccucaa uaccaaaaac ggauccauca gccuaaugug ccuggcccua     2040

ggcggagucc ugaucuuccu cucaacagca gucagcgcag acugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  9445
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9445
gggagaaagc uuaccaugcg aggcgcggac acgucagucg gcauagucgg ucuacuacuu       60

acaaccgcca uggcagccgc cguuacacgc cggggauccg ccuacuacau guaccucgac      120

cgcaacgacg ccggcgaagc aaucucguuc ccaaccacac uaggcaugaa caaaugcuac      180

auccaaauua uggaccuagg acacaugugc gacgccacca ugagcuacga gugcccaaug      240

cuagacgaag gaguggagcc agacgacgug gacugcuggu gcaacacuac aucgaccugg      300

gucgucuacg ggaccugcca ccacaagaaa ggagaagcac gaaggucaag acgagcugua      360

acacuaccaa gccacuccac aaggaaauua cagacccgaa gccaaacaug gcucgaaucc      420

agggaauaca ccaaacaucu cauucgcgua gaaaacugga uauuccgcaa cccaggcuuc      480

gcacuagcgg cggccgcgau agcuuggcuc cucgggagcu ccaccucgca aaaaguaauc      540

uaccuaguca ugauccuacu cauagcuccc gcguacagca ucagaugcau cggcguaagc      600

aaccgcgacu ucgucgaagg caugagcggc ggcaccuggg uagacauagu acucgaacac      660

gggggcugug ucaccguaau ggcacaagac aaaccaacgg uggauaucga gcuagucaca      720

acgaccgucu ccaacauggc cgaaguaaga agcuauugcu acgaggcauc cauaagcgac      780

auggcaagcg acucgcgcug cccaacgcaa ggugaagcau accuagacaa acaaagcgac      840

acacaauacg uaugcaaacg cacccucguc gacagagggu ggggcaaugg augcggacuc      900

uucggcaaag gcucccucgu cacaugcgca aaauucgccu gcuccaaaaa gaugacaggc      960

aaaucaaucc aacccgaaaa ccuagaguac cgaauaaugc ucagcgucca cggaagccaa     1020

cacagcggaa ugauagucaa cgacacagga cacgaaaccg acgaaaaccg cgcaaaaguc     1080

gaaaucacac caaacagucc aagagcggaa gcaacacuag gcggauucgg aucacuagga     1140

cuagauugcg agccacgcac aggacuagac uucagcgacc uauacuaccu gacgaugaac     1200

aacaaacacu ggcucguaca caaagagugg uuccacgaca ucccccuccc auggcacgcc     1260

ggcgccgaca ccggcacccc gcauuggaac aacaaagaag cccuaguaga guucaaagac     1320

gcacacgcaa agcgccaaac cgucgucgua cuaggaagcc aagaaggagc gguccacaca     1380

gcccuagccg gcgcccuaga agcugaaaug gacggagcaa aaggaagacu aagcagcggu     1440

caccucaaau gcagacucaa aauggacaaa cuacgccuca aaggcguauc guacucccuc     1500

ugcaccgccg ccuucacauu caccaaaaua ccagcagaga cacuacacgg aaccgugaca     1560

guggaagucc aauacgccgg caccgaugga ccaugcaaag uacccgcaca aauggcagua     1620

gacaugcaaa cccugacacc cgucggcaga cuaaucacag ccaacccagu cauaaccgaa     1680

agcaccgaga acagcaagau gaugcucgag cucgacccgc cauucgggga cagcuacauc     1740

gucauaggag ugggagaaaa gaaaaucacc caccacuggc accgaagcgg cucaacaaua     1800

ggaaaagcgu ucgaagcgac cguccgagga gccaaacgca uggcaguacu cggugacaca     1860

gccugggacu ucggcucagu aggcggcgcc cuaaacucuc uaggaaaagg cauacaccaa     1920

aucuuuggcg cagccuucaa aucacucuuc ggcggcauga gcugguucuc ccagauccua     1980

auaggcacac uacuaaugug gcucggacuc aacaccaaaa auggcucgau cagccugaug     2040

ugccucgcac uaggcggcgu acugaucuuc cuauccacag cgguaagcgc cgacugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  9446
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9446
gggagaaagc uuaccaugcg cggccuaucg ucacggaaaa gacggagcca cgacgugcua       60

acagugcaau uccucauacu cggcaugcua cuaaugaccg gaggcgucac ccgccgaggc      120

ucagccuacu acauguaccu agacaggaac gacgccgggg aagccauaag uuuccccacg      180

acacugggca ugaacaaaug cuauauacaa aucauggauc uaggccacau gugcgacgcc      240

accaugagcu acgaaugccc gaugcuagac gaaggagucg agccagacga cguggacugu      300

uggugcaaca caacauccac auggguaguc uacggaacau gccaccauaa gaaaggagaa      360

gcccgacgcu caaggagagc cgugacccuc ccaagccacu cgaccagaaa acuccaaaca      420

agaagccaaa cguggcucga aucgagggaa uacacgaagc accuaauaag agucgaaaac      480

uggauauucc gaaacccagg auucgcacug gccgcggccg ccaucgccug gcuacucgga      540

agcagcacga gccaaaaagu aauuuaucuu guaaugaucu ugcugauagc cccggccuac      600

agcauccgau gcaucggcgu uucgaauaga gacuucguag aaggcaugag cggcgguacc      660

uggguagaua ucguccucga acacggggga ugcgugaccg uaauggccca ggacaaaccg      720

accgucgaca uagagcuagu aaccacaaca gucagcaaca uggcagaagu caggaguuac      780

ugcuaugaag caucaauauc agacauggca agcgacagca gaugcccuac acaaggggag      840

gcauaccucg acaaacaaag ugacacccaa uacgucugua aaagaacccu agucgaccgc      900

ggauggggga auggcugcgg acuauucgga aaagggagcc uagugacaug cgccaaauuc      960

gcaugcucaa agaaaaugac aggaaaaagc auccaaccag aaaaccucga guacagaaua     1020

augcucagcg uccacggguc acagcacagc ggcaugauag uaaacgacac cggacacgaa     1080

accgacgaaa accgagccaa aguagaaaua accccuaaua gccccagagc ugaggcaacc     1140

cuaggaggcu ucggcagcuu gggacucgac ugcgaacccc gaacgggacu cgacuucucc     1200

gaccuauacu accucaccau gaacaacaaa cacuggcucg ugcacaaaga augguuccac     1260

gacauccccc ugcccuggca cgccggcgcc gacacaggaa cgccucacug gaacaacaag     1320

gaagcccuug uagaguucaa agacgcgcac gcaaaacgcc aaacgguggu cgugcucgga     1380

agucaagaag gagccgugca cacggcacua gccggcgcgc ucgaagccga aauggacgga     1440

gcaaaggggc gacugagcag cggacaccug aaaugccggc ugaaaaugga caaacugaga     1500

cuaaaaggag ucagcuacag ccuaugcacc gccgcauuca cauucacaaa aauaccggcc     1560

gaaacacuac acgguacagu cacgguagaa guccaauacg cggggacaga cggacccugc     1620

aaagucccag cgcaaauggc gguagauaug cagacacuca cacccguggg uagauuaaua     1680

acagcaaacc ccgucaucac agaaagcacg gaaaacagca aaaugaugcu agaacuagac     1740

ccacccuucg gagauucaua cauagucaua ggcguaggcg aaaagaaaau aacccaccac     1800

uggcaccgca gcggcagcac aaucggaaaa gcauucgaag caacggugag gggagccaaa     1860

cgaauggcgg uccucggaga uacagccugg gacuucggaa gugucggggg ugcacugaac     1920

agccuaggca aaggcauaca ccaaaucuuc ggagcagcgu ucaaaagccu cuucggggga     1980

augagcuggu ucucccaaau auugauagga acucuacuca uguggcucgg gcuaaacacu     2040

aaaaauggca gcaucagccu caugugccug gcccucggag gcguccucau auuccuaagc     2100

acagccguca gcgccgacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9447
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9447
gggagaaagc uuaccaugcg aggccuuagc ucccgcaaac gacgcagcca cgacguacua       60

accguccaau uccuaauacu aggcaugcuc cucaugacag gcgccguaac aagacggggu      120

uccgcauacu acauguaccu cgacaggaac gacgcgggag aagccauuuc cuucccaacc      180

acacucggca ugaacaaaug cuacauacaa auaauggauc ucggacacau gugcgacgcc      240

accaugucau acgaaugccc aaugcuagac gaaggagugg aacccgacga cguagacugc      300

uggugcaaca ccacauccac cuggguaguc uacggaacgu gccaccacaa gaaaggagaa      360

gcacggcgaa gccgacgagc cguaacccuc cccucccaca gcacucgcaa acuccaaaca      420

cgaucacaaa cauggcuaga aucccgcgaa uacaccaaac accuaauacg cgucgaaaac      480

uggaucuuuc gaaacccagg cuucgcccuc gcagcagccg cgauagcaug gcuucuugga      540

agcagcacaa gucaaaaagu aaucuacuua guaaugaucc ugcuuauagc gccugcauau      600

ucgauacggu gcaucggggu aagcaaccgc gacuucgucg aaggaauguc aggaggcacc      660

uggguagaca uuguacucga acacggagga ugcguaaccg uaauggccca agacaaaccu      720

acagucgaca uagagcucgu aacgaccaca guaagcaaca uggcagaggu ccgaucauac      780

ugcuacgaag ccucaauaag ugacauggcc ucagacucac gcugcccaac ccaaggcgag      840

gccuaccuag acaagcaauc cgacacacag uacgucugca aacgcacacu cgucgacaga      900

ggguggggca acgguugcgg ccuuuucggc aaaggaucac ucguaacaug cgcaaaauuc      960

gccugcucca agaaaaugac uggaaaaagc auacaaccag aaaaccuaga guacagaaua     1020

augcuauccg uacacgggag ccagcacuca ggaaugaucg ucaacgacac gggacacgaa     1080

accgacgaaa accgcgcaaa aguagaaaua acacccaacu caccccgagc agaagcgacc     1140

cuaggaggau ucggaucccu cggacuagac ugugaaccca gaacaggccu cgacuucagc     1200

gaccucuacu accucaccau gaacaacaaa cacuggcuug uccacaaaga gugguuccac     1260

gacauccccc uacccuggca cgccggagca gacaccggca ccccacacug gaacaacaaa     1320

gaagcccucg ucgaguucaa agacgcgcac gcaaaacgac aaaccgucgu cguucuugga     1380

ucccaggaag gagccgucca uacagcacua gcaggcgccc uggaagccga aauggacggc     1440

gccaaggggc ggcuaucauc aggccaccua aaaugucgcc uaaagaugga caaacuccga     1500

cuaaaagggg ucucauacuc ucuaugcacc gcggcauuca ccuucaccaa gauccccgca     1560

gaaacccuac acggaaccgu cacaguagaa guacaauacg ccggcacaga cggccccugc     1620

aaggucccag cccaaauggc cguagacaug caaacacuga ccccaguagg acgacuaaua     1680

accgcgaacc ccguaauaac cgaaucgacc gaaaacucaa aaaugaugcu cgaacuagac     1740

ccaccguuug gcgacagcua caucgucauc ggcgucggag agaaaaagau aacccaccac     1800

uggcacaggu ccggaagcac caucgguaag gcuuucgaag caaccguccg cggagccaaa     1860

cgcauggccg uccuaggaga cacagcaugg gauuucggca gcgucggcgg agcccugaac     1920

agccucggaa aaggaauaca ccaaauauuc ggagccgcgu ucaaaagccu auucgggggc     1980

auguccuggu ucucacaaau ccuaaucggc acacuacuga uguggcucgg ccucaacacc     2040

aaaaacggga gcauuagccu caugugucua gcucuaggcg gcguacuaau cuuccuaucc     2100

acagcaguga gcgcggacug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9448
<211>  2335
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9448
gggagaaagc uuaccaugcg caucauaaac gccagaaaag aaaagaaacg aaggggagcc       60

gacacaucag uaggcaucgu cggacuacuc uugaccaccg caauggccgc ggaagucacc      120

agaaggggcu cagcauacua cauguaccua gaccguaacg acgccggaga agcaaucucc      180

uuccccacca cccuuggaau gaacaaaugc uacauacaaa uaauggaucu aggccauaug      240

ugcgacgcca ccauguccua cgaaugcccc augcucgacg aaggaguaga gcccgacgac      300

guggacuguu gguguaauac caccagcaca ugggucgucu augggaccug ccaccacaag      360

aaaggcgaag cacgaagaag ucgacgagca guaacacuac cgagccacag cacccgaaaa      420

cuacaaacaa gaagucaaac auggcuagaa agccgagaau acacgaaaca ccuaauaaga      480

guagagaacu ggauauucag aaacccaggg uucgcccucg ccgccgcggc caucgcaugg      540

cugcuaggcu ccagcacauc gcaaaaagua auauaccuag ucaugauucu acuaaucgca      600

cccgcauaca gcauccgcug cauaggcguc ucgaaccgcg acuucgucga gggcauguca      660

ggaggcacau ggguggacau cgucuuagaa cacgguggau gcgucaccgu cauggcccaa      720

gacaaaccca ccguagacau cgagcuggua accaccaccg uauccaacau ggccgaagug      780

cgguccuacu gcuacgaagc cucaauaagc gacauggccu ccgacagcag augcccaacc      840

caaggggagg cguaucucga caaacaaagc gacacccaau acgucugcaa acgaacacuc      900

guagaccgcg gguggggaaa cggcugcgga cucuucggaa aaggcagccu cguaaccugu      960

gccaaauucg caugcucgaa gaaaaugacc ggaaaaucca uacaaccaga gaacuuagaa     1020

uaccgcauca ugcuaagcgu acauggcagc caacacagcg gcaugauagu aaacgacaca     1080

ggccaugaaa cagacgaaaa ccgagcaaaa gucgaaauca cccccaacag cccgagagca     1140

gaagccacac uaggaggauu cggcagccua ggccuggacu gcgaaccaag gacaggccuc     1200

gacuucagcg accucuacua ccucaccaug aacaacaagc acuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu ccccuggcac gcaggcgccg acacagggac accccauugg     1320

aauaacaaag aagcacuggu agaguucaaa gacgcccacg ccaagagaca aaccguagua     1380

guacuaggcu cccaggaagg ggcaguccac acagcacuag cgggagcacu ggaagcagaa     1440

auggacggcg ccaaagggcg acuaagcagc ggccaccuga aaugcagacu uaaaauggau     1500

aaacuacgac uaaagggcgu cagcuacagc cucugcaccg cugccuucac auucacgaaa     1560

auccccgccg aaaccuuaca cggcacgguc acagucgaag uacaauacgc cggaaccgac     1620

gguccaugca aaguacccgc acaaauggcc gucgacaugc agacacuaac acccgucgga     1680

cggcuaauaa cagccaaccc cguuaucaca gaaucgaccg aaaauucaaa aaugaugcuc     1740

gaacuagacc caccguuugg agacuccuac aucguaaucg gcgucggaga aaagaaaauc     1800

acacaccacu ggcaccgcag cggcuccacg auagggaaag cauucgaggc caccguccga     1860

ggagcaaaaa gaauggccgu ccucggcgac acagcauggg acuucggcag cgucggaggc     1920

gcgcucaaca gucucggaaa aggcauccac caaauauucg gcgcagcauu caaaagccuu     1980

uucggcggca ugagcugguu uucacaaauc cuaauaggaa cccuccucau guggcucggc     2040

cuaaacacca aaaacggguc aauaagccua augugccugg cccucggcgg gguccuaaua     2100

uuccucucca cggcagucag cgcaugagga cuaguuauaa gacugacuag cccgaugggc     2160

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uagaucuaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2280

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2335


<210>  9449
<211>  2329
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9449
gggagaaagc uuaccaugau aaacgcccga aaagagaaaa agcgccgagg agccgacaca       60

uccguaggua uagucggccu cuuauuaaca acagcaaugg cugccgaagu aacaagacgc      120

ggcagcgcau auuacaugua ccuugaccga aacgaugcag gggaagcaau aucauuucca      180

acaacacuug gcaugaacaa augcuauauc caaaucaugg acuugggaca caugugcgac      240

gccacaaugu cauacgaaug cccgaugcua gacgaagggg uggaaccgga cgacguugac      300

ugcuggugca acacaacuuc aaccugggua gucuacggaa ccugccacca caagaaaggc      360

gaagcaagaa gauccagacg ggccgucaca cuccccagcc acagcacuag aaaacuacaa      420

acccgaagcc aaacauggcu cgaaagccgc gaauacacaa agcaucugau ccgaguggaa      480

aacuggaucu uccgaaaccc cggcuucgcc cucgccgccg cagccauagc auggcuccug      540

ggauccucca cgucacaaaa agugauauac cuaguaauga uccugcucau agccccggcc      600

uacuccauac gcugcauagg cguaagcaac cgagacuucg uagagggaau gucagggggc      660

accugggucg acaucguccu agaacacggg ggcugcguca cagucauggc gcaagacaag      720

cccacagucg acaucgagcu cgucacaaca accguaucaa acauggcaga gguaaggucc      780

uacugcuacg aagcgucaau cucagacaug gccagcgacu cccguugccc cacacaaggc      840

gaggccuacc uagacaaaca auccgacaca caauacguau gcaaacgaac acucguagac      900

cggggcuggg gaaacggcug cggacucuuc ggcaaaggca gccucgucac augcgcgaaa      960

uucgccugcu caaagaaaau gaccggaaaa agcauccaac ccgagaaccu agaauaccga     1020

auaaugcuaa gcguacacgg gucccaacac uccggaauga ucgucaacga caccggacac     1080

gaaaccgacg aaaacagagc uaaaguagaa aucacgccaa acucgccacg cgcagaagcc     1140

acgcucggag gauucggauc acuagggcua gacugcgagc cacgcaccgg gcuagacuuc     1200

uccgaccuau acuaccuaac caugaacaac aaacacuggc uuguucacaa agaaugguuc     1260

caugacaucc cccuccccug gcacgcagga gccgacacag gaaccccaca cuggaacaac     1320

aaagaagccc uaguagaguu caaagacgca caugcaaaac gccagaccgu cgucguacua     1380

ggaucccaag aaggagcugu ccauaccgca cucgccggcg cccucgaagc cgaaauggac     1440

ggagccaaag ggcgccuaag cuccggacac cucaaaugcc gacuaaaaau ggacaaacua     1500

agacugaaag gcgucagcua cagccuaugc acagccgcau ucacauucac caaaauucca     1560

gcagaaacgc uccacggaac agucaccgua gaaguacaau acgccggcac agacggcccc     1620

ugcaaagugc cagcccaaau ggcgguagac augcaaacac ucacaccagu cggccgccua     1680

auaacggcua acccaguaau caccgaaagc accgaaaaua gcaaaaugau gcuugaacuc     1740

gacccgcccu ucggagacag cuacaucguc aucggcgucg gggaaaagaa aauaacacac     1800

cacuggcacc gaagcggguc cacaaucggc aaagccuucg aagcaaccgu aaggggcgca     1860

aaacgcaugg ccguacucgg agacacggcc ugggacuucg guuccgucgg aggcgcacua     1920

aacagccugg gcaaaggcau ccaucaaauc uucggagcag cauucaaauc acuuuuuggg     1980

ggaaugagcu gguucuccca aauccugauc ggcaccuuac uaauguggcu gggccuuaac     2040

accaaaaaug gcagcaucuc gcucaugugc cucgcacucg gaggaguccu aaucuuccug     2100

agcacagccg uauccgccug aggacuaguu auaagacuga cuagcccgau gggccuccca     2160

acgggcccuc cuccccuccu ugcaccgaga uuaauagauc uaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2280

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2329


<210>  9450
<211>  2332
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9450
gggagaaagc uuaccaugau aaucaacgca agaaaggaaa agaaacgccg cggugccgac       60

acaucaguag gcauagucgg ccuccuccuc acgacagcua uggccgcaga aguaacacga      120

cgcggaagcg cguacuacau guaccucgac cgcaacgacg ccggagaagc gaucuccuuc      180

ccgaccacac ucggcaugaa caaaugcuac auccaaauca uggaucucgg acacaugugc      240

gacgccacca uguccuacga gugcccaaug cuagacgaag gagucgaacc cgacgacgua      300

gauugcuggu gcaacacgac cuccacaugg gucguuuacg gaaccuguca ccacaagaag      360

ggcgaagccc gacgaagccg cagagcaguc acccuaccca gccacuccac ucgcaaacuc      420

cagacacgaa gccaaacaug gcucgagagc cgagaguaca caaaacaccu cauaagggua      480

gaaaacugga ucuuccgaaa uccaggcuuc gcccuagccg cagccgccau cgcguggcuu      540

cuaggcucaa gcacgucaca aaaggucauc uaccucguca ugauccuacu aaucgcucca      600

gccuauucaa uccggugcau agguguaagc aaccgagacu ucguagaagg caugucagga      660

ggaacauggg uagacaucgu ccuggaacau ggcggaugcg uaaccgucau ggcccaagac      720

aaacccacag uagacaucga acucgugacc accaccguaa gcaacauggc agaaguccga      780

agcuacugcu acgaggcuuc aaucagcgac auggcaucag acagcagaug ccccacacaa      840

ggcgaagccu accuagacaa gcagucagac acccaauaug uaugcaaacg cacccuaguc      900

gauaggggau ggggaaacgg augcggacuc uucgguaaag gcagucucgu gaccugcgcg      960

aaauucgcgu gcuccaagaa aaugacgggc aaaagcauuc aacccgaaaa ccuagaauac     1020

agaaucaugc uaucagucca cggcucccaa cacuccggca ugauugucaa cgacaccggc     1080

cacgaaaccg acgaaaaccg agcaaaaguc gaaaucacac ccaacagccc gcgcgccgaa     1140

gccacacucg gcggcuucgg cagccugggc cucgacugug aaccccgcac aggacuagac     1200

uucagcgacc ucuacuaccu gacaaugaac aacaaacacu ggcucgucca caaagaaugg     1260

uuccacgaca uuccccuccc cuggcacgcc ggcgcagaua ccggaacccc acacuggaac     1320

aauaaagaag cacucguaga guucaaggac gcgcacgcaa aacggcaaac agucgugguc     1380

cugggcucgc aagaaggcgc aguccacaca gcccucgccg gcgcccuaga agccgaaaug     1440

gacggagcaa aagguagacu cagcucagga caccuaaaau gcagacucaa gauggacaaa     1500

uuacgccuca aaggcguauc uuacucccua ugcaccgccg cauucaccuu uaccaaaauc     1560

ccggcagaaa cgcuccaugg aacagucacc gucgaggucc aauacgccgg aaccgaugga     1620

ccuugcaaag uccccgccca aauggcgguu gacaugcaga cccugacccc cgucggccga     1680

cucaucaccg ccaacccagu caucacugaa agcacagaaa acuccaaaau gaugcucgaa     1740

cucgacccac ccuucggcga caguuacauu gucaucggcg ucggggaaaa gaagaucaca     1800

caccauuggc accgguccgg aagcacgauu ggaaaggccu ucgaagcaac cgucagagga     1860

gccaaaagaa uggcgguacu cggcgacacc gcaugggacu uuggcuccgu cggcggagca     1920

cuuaacucac ucggaaaagg cauacaccaa auauucggug cugccuucaa aucacucuuc     1980

ggcggcauga gcugguucuc acagauacua aucggaacac uacuaaugug gcuaggccuc     2040

aacaccaaaa acggaagcau aucacuuaug ugccuagccc ucggcggagu acuaaucuuc     2100

uuguccacag cagucuccgc cugaggacua guuauaagac ugacuagccc gaugggccuc     2160

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2280

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2332


<210>  9451
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9451
gggagaaagc uuaccaugaa cgccagaaaa gaaaagaaac gaagaggcgc cgacaccagc       60

guuggaaucg ucggacuacu acuaaccacc gcgauggcag ccgaagucac gagaagggga      120

agcgccuacu acauguaccu agacagaaac gacgcaggcg aggcaauaag uuuccccaca      180

acgcuaggaa ugaauaaaug uuacauacaa auaauggauc ucggacacau gugcgacgca      240

acaaugagcu acgaaugccc aaugcuagac gaaggagucg aaccagacga uguggacugc      300

uggugcaaca caacuagcac augggucguc uacggcacau gccaccacaa gaaaggagaa      360

gcacgaagga gucgcagagc cgucacgcua cccagccacu caacgagaaa acuccagacc      420

aggagccaaa cguggcuaga gagcagagaa uacacaaaac accuaauaag agucgaaaac      480

uggaucuucc gaaacccagg cuucgcccuc gccgccgcag ccauagccug gcuccuaggu      540

ucuaguacca gccaaaaagu aauauaccua gugaugaucc uacugauagc acccgcauac      600

uccauccguu gcauaggagu cagcaaccgc gacuucguag aaggaaugag cggaggaacc      660

ugggucgaua ucguacuaga acacggcggc ugcgucacag ucauggccca agacaaacca      720

accgucgaca uagaacucgu gacaacaaca guuagcaaca uggcggaagu gcgaagcuac      780

ugcuaugaag ccuccauauc agacauggca ucggacagcc gauguccaac acaaggagaa      840

gccuaccuag acaagcaauc cgacacacaa uacguaugca aaagaacccu agucgaucga      900

ggcuggggca acgggugcgg ccuuuucgga aaaggaagcc uggucacaug cgccaaauuc      960

gcgugcagca agaaaaugac aggcaaauca auccaaccag aaaaccucga auaucgaauc     1020

augcucucgg uacacggcag ccaacacucc ggaaugaucg uaaacgacac aggucacgag     1080

acagacgaaa acagagcaaa agucgaaauc acgcccaaca gcccaagagc cgaagcaaca     1140

cucggcgggu ucgggagccu aggacuagac ugcgaacccc gaacaggccu cgacuucucg     1200

gaccucuacu aucuuaccau gaacaacaaa cacuggcuag uccacaaaga augguuccac     1260

gacauccccc uccccuggca cgcaggggca gacaccggaa cgccgcauug gaacaauaaa     1320

gaggcucuag ucgaguucaa ggacgcacac gcgaaaagac aaaccgucgu cguauuaggc     1380

ucacaagagg gcgccgucca cacagcgcuc gcaggggcac uagaagccga gauggacggc     1440

gcgaaaggaa ggcuaucauc aggucaccuc aaaugccggc uuaagaugga caaacugaga     1500

cuaaaagggg uauccuauuc acucugcaca gcagcauuca cauucacaaa aauccccgca     1560

gaaacacugc acggaacagu aaccguagaa guccaauacg caggcaccga cggcccaugc     1620

aaaguaccug cccaaauggc cguagacaug cagacccuaa cgcccgucgg ccggcucauc     1680

acggccaacc cgguaauaac ugaaucaaca gaaaacucua aaaugaugcu ggaacuggac     1740

ccacccuucg gcgacaguua cauagucauc ggugucggug aaaagaaaau cacccaccac     1800

uggcacagaa gcggaagcac uauaggcaaa gcguucgaag ccacaguaag aggcgcaaaa     1860

aggauggcag uacuaggaga caccgccugg gacuucggcu caguaggagg agcacuaaac     1920

ucgcucggca agggaaucca ccaaauauuc ggagccgcau ucaagagccu auucggcgga     1980

augagcuggu ucucacaaau ccuaaucgga acgcugcuaa uguggcucgg ccuaaacaca     2040

aagaacggca gcaucagccu aaugugccuc gcccucgggg gcguccuuau auucuugagc     2100

acagcaguaa gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9452
<211>  2323
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9452
gggagaaagc uuaccauggc caggaaagag aaaaagagaa gaggcgcgga cacaucagua       60

ggaauagucg gacuacugcu aacaaccgcc auggcagcag aaguaacccg aagaggcuca      120

gccuacuaca uguaccucga ccgaaacgac gccggagaag ccauaucauu ccccaccacc      180

cucggaauga acaaaugcua cauccagauc auggaccucg gccacaugug cgaugcaacc      240

augagcuacg aaugcccaau gcucgacgaa ggcguagaac cagacgacgu agacugcugg      300

ugcaacacaa ccaguacgug gguagucuau ggcacaugcc accacaagaa aggcgaagca      360

cgcagaagcc gccgcgcagu cacucuccca ucucacagca ccagaaaacu ccagacaagg      420

agccagaccu ggcuggaauc ccgagaauac accaaacacc uaauaagggu cgaaaacugg      480

auuuuccgca acccaggcuu cgcacucgca gccgcagcca uagccuggcu acugggcagc      540

agcacaagcc aaaaagugau cuaucucguc augauacucc ugauugcccc agcuuacucc      600

aucagaugca ucggcguaag caaccgagac uucgucgagg ggaugucggg aggaaccugg      660

guugacaucg uacucgaaca cggcggaugc guaaccguaa uggcucaaga caaacccacc      720

guugacauag aacuggugac aaccacagua uccaacaugg cagaggugag aucauacugc      780

uacgaggcuu caaucucuga cauggccucg gacagccgcu gccccacaca gggggaagcg      840

uaccucgaca aacaaucaga cacacaguac gucugcaaac gaacccucgu agaccgcgga      900

uggggcaacg ggugcgggcu auucggcaaa ggaucacucg ucacaugcgc aaaauucgcu      960

ugcucgaaga aaaugacagg caaaagcaua caaccagaga accucgaaua cagaauaaug     1020

cugucaguac acggcucaca acacagcgga augauuguca acgacacagg acacgagacc     1080

gacgaaaaca gggccaaagu agaaauaacc cccaacagcc cccgagccga ggcaacacuu     1140

ggaggauucg gcucccuagg auuagacugc gaaccgcgca ccggacugga cuuuagcgac     1200

cuauacuacc ucacaaugaa caacaaacac uggcuagucc acaaagagug guuccacgac     1260

auaccccucc cauggcaugc cggagcagac acaggaaccc cacacuggaa caacaaagaa     1320

gcgcugguag aguucaaaga cgcccacgca aaacgccaaa cagucguggu ccucggcagc     1380

caagaaggcg ccguccacac cgcacucgcc ggcgcacuag aagcagaaau ggacggcgcg     1440

aaaggccggc ucagcuccgg gcaccuaaaa uguagacuaa aaauggauaa gcuacgccua     1500

aaagggguau cauacagccu augcaccgcc gccuucacau ucacaaaaau accggcagaa     1560

acacugcacg gaacaguaac agucgaagua caauacgcag gaaccgaugg cccaugcaaa     1620

guuccagccc aaauggcagu cgacaugcaa acacugaccc cggucggaag acuuaucaca     1680

gcaaaccccg ucaucacaga aucaaccgaa aacagcaaaa ugaugcucga acuagaccca     1740

ccauucggcg acaguuacau agugauaggc guaggagaga agaaaaucac ccaucacugg     1800

cacagauccg ggagcacuau agggaaagcc uucgaggcca caguaagggg agccaaacgc     1860

auggccgucc ucggugacac agcaugggac uucggcucag uaggcggcgc ccucaacucc     1920

cucggaaaag gaauccacca aauauucggg gccgcauuca agagccuguu cgggggcaug     1980

agcugguucu cccaaauccu aauaggcacc cuccucaugu ggcuaggccu caauaccaaa     2040

aacggaagua ucucacucau gugccuagca cuaggcggcg uccucauauu ccucagcaca     2100

gccguuagcg cgugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     2160

ccuccucccc uccuugcacc gagauuaaua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2280

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2323


<210>  9453
<211>  2320
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9453
gggagaaagc uuaccaugcg aaaagagaag aaaagacgcg gagcagacac cagcgucgga       60

aucgucggcc uucuccucac uacagccaug gccgccgaag ucacgagacg cggauccgca      120

uacuacaugu aucuagaccg aaacgacgcc ggcgaggcaa ucucauuccc caccacccuc      180

ggcaugaaua aaugcuauau ccagauaaug gaccucggcc acaugugcga cgcaacaaug      240

agcuacgaau gccccaugcu cgacgaggga guagaacccg augacguuga cugcuggugc      300

aacacaaccu cgaccugggu cguguacggc acaugccacc acaagaaagg agaagcgaga      360

cgcucccguc gagcaguaac ccuuccaucc cacagcaccc ggaaacucca aacacgcucc      420

caaaccuggc ucgaaucccg cgaauauaca aaacaucuaa uccgaguaga aaacuggauc      480

uuccgcaacc cgggauucgc ccuggcagcg gccgccaucg ccuggcuacu cgguagcucc      540

accagccaaa aaguaaucua ccucguuaug auacuacuaa ucgccccagc auacucaaua      600

cgcugcaucg gcguaagcaa ccgagacuuc gucgaaggaa ugucaggcgg caccuggguc      660

gacaucgucc uagaacacgg cggaugcguc accgucaugg cgcaagacaa accaaccgua      720

gacauagagc uaguaacaac caccguauca aacauggccg aaguccgaag cuacugcuac      780

gaagcaucga uauccgacau ggccuccgac ucacgcugcc ccacccaagg agaagccuac      840

cuagacaaac agucggauac gcaauacguc ugcaaacgaa cccuagucga ccgaggcugg      900

ggaaacggcu gcggccucuu cggaaaaggg ucccuaguaa ccugcgcaaa auucgcaugc      960

agcaagaaaa ugacaggcaa guccauccag cccgaaaacc ucgaauaucg aauaaugcuc     1020

uccguccacg gcucacaaca cagcggaaug aucgucaacg acaccggaca cgaaaccgac     1080

gaaaaccgag caaaagucga gauaacccca aacucaccuc gcgcugaggc aacacucgga     1140

ggcuucggcu cccucggacu cgacugcgaa ccacgaaccg ggcuagacuu cagcgaucua     1200

uauuaccuca ccaugaacaa caaacacugg cuaguccaca aagaaugguu ccacgacaua     1260

ccacuccccu ggcacgccgg cgcagacacc ggcacgcccc acuggaacaa caaagaagcc     1320

cuggucgagu ucaaagacgc ccacgccaaa cgucaaaccg ucgugguacu cgggucccaa     1380

gaaggagcag uccacacagc acuugcgggc gcccuggaag ccgaaaugga cggcgccaaa     1440

ggacgucucu ccagcggaca ccuaaagugc cgccugaaaa uggacaaacu acgacuaaag     1500

ggagucucau acagcuuaug caccgccgcc uucacauuua ccaaaauacc cgcagaaacg     1560

cuacacggca ccguaaccgu cgaaguacaa uacgcgggaa cagacggacc cugcaaagua     1620

ccugcucaaa uggccguaga caugcaaaca cucacaccag ucggaagacu aaucacagca     1680

aaccccguaa ucaccgaaag caccgaaaau uccaaaauga ugcucgaacu agacccaccg     1740

uuuggagacu cuuacaucgu aauaggagug ggcgaaaaga aaaucacaca ucacuggcac     1800

cgaucgggau cgaccauagg uaaagccuuc gaagcaacag ugcgaggcgc caaacgaaug     1860

gcuguacuug gagacaccgc augggauuuu ggcuccguug gaggagcccu uaacagccuc     1920

ggcaaaggca uacaccagau uuucggcgca gccuucaaau cccucuucgg cggcaugucc     1980

ugguucuccc aaauccucau cggaacccua cucauguggc uaggccucaa cacgaaaaac     2040

ggaucaauau cccucaugug ccuagcacug ggcgguguac uaauauuccu cuccaccgcc     2100

gucucagcgu gaggacuagu uauaagacug acuagcccga ugggccuccc aacgggcccu     2160

ccuccccucc uugcaccgag auuaauagau cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     2280

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2320


<210>  9454
<211>  2317
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9454
gggagaaagc uuaccaugaa agagaaaaag aggagaggcg cggacacguc cgucgguaua       60

guaggccuac uucuaacaac cgccauggcc gcagaaguaa cccgucgagg aagcgccuac      120

uacauguacc ucgaccgaaa cgacgccggc gaagcgauca gcuuccccac cacccucggc      180

augaacaaau guuacaucca gauaauggac cucggccaca ugugcgacgc cacgauguca      240

uacgaaugcc ccaugcuuga cgaaggcguu gaaccagacg acgucgacug cuggugcaac      300

acuacaagca ccuggguggu cuacggcacu ugccaccaca aaaagggaga agcacgccgc      360

agccgucgag ccguaacccu ucccucacac agcacccgaa agcuccaaac ccgcucccaa      420

accuggcuag aaucucgaga auacaccaaa caccucauua gagucgaaaa cuggauauuc      480

cgcaauccag gcuucgcccu agcagcagcc gcaaucgccu ggcuucucgg auccucaaca      540

ucacagaaag ucauauaccu cguuaugaua cuccuaaucg ccccagccua cagcaucaga      600

ugcaucggcg ucagcaaccg agacuucgua gaaggcauga gcggaggaac auggguagac      660

aucguccucg aacacggagg augcgucacc guaauggccc aagacaaacc cacaguugac      720

auugagcuag ucacaaccac cgugagcaac auggccgaag ugcgcagcua cugcuacgaa      780

gccuccauau cagacauggc aucagacagc cgcugcccua cacagggaga agcauaccug      840

gacaaacagu ccgacacaca auacgucugc aaacgaaccc ucguagaccg aggcugggga      900

aacggaugcg gacuauucgg caaaggcucc cucgucacau gcgcaaaguu cgccugcagc      960

aagaaaauga ccggcaaauc uauccagccc gagaaccucg aauacagaau aaugcucucc     1020

guacacggau cccaacacag cggcaugauc gucaacgaca caggacacga aacugacgaa     1080

aaucgcgcga aagucgaaau cacacccaac uccccucgag cggaagcuac ccugggcggc     1140

uucggcagcc uaggccucga uugcgaacca cgaacaggcu uagacuucuc cgaccucuac     1200

uaccuaacga ugaacaauaa acacuggcua guccacaaag aaugguucca cgacauaccg     1260

uuaccguggc acgccggggc cgauaccgga acaccacauu ggaacaacaa agaagcacug     1320

guagaguuca aagacgccca cgcaaaacgc caaaccgucg ucguacucgg gucccaagaa     1380

ggcgcagucc acacagcccu cgcaggagcc cucgaagccg aaauggacgg cgcaaaaggc     1440

cgccucagca guggucaccu aaaaugcaga cuaaaaaugg acaaacuccg acuaaaaggc     1500

gucucguacu cccuguguac cgcagcuuuc acuuucacca agauccccgc agaaacccuc     1560

cacggcaccg ucaccguaga agugcaauac gcagggaccg auggccccug uaaaguaccg     1620

gcccaaaugg cagucgacau gcagacccug acacccgugg gccggcucau cacagcaaac     1680

cccguaauaa cagaauccac cgaaaacucc aagaugaugc uagagcuaga ucccccauuc     1740

ggcgacagcu acauagucau cggaguagga gaaaagaaaa ucacacacca cuggcaccgg     1800

uccggcucca ccauaggcaa agcauuugaa gcuacagucc gcggcgccaa acgcauggca     1860

guacuuggcg acaccgccug ggacuucggc uccguaggag gggcgcucaa cagccuaggg     1920

aaaggaaucc accaaaucuu uggagccgca uucaaauccc uauucggcgg cauguccugg     1980

uucucucaaa uccugaucgg cacccuacuc auguggcuag gccucaacac caaaaacggc     2040

uccaucuccc uaaugugccu ggcacucgga ggaguacuca ucuuccuauc cacagccguc     2100

uccgcaugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     2160

ccccuccuug caccgagauu aauagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2280

ccccccccca aaggcucuuu ucagagccac cagaauu                              2317


<210>  9455
<211>  2314
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9455
gggagaaagc uuaccaugga gaagaaaaga agaggagcag acaccagcgu aggcaucgua       60

ggccuccuac ucacaaccgc aauggccgcc gaaguaaccc gccggggcuc cgcauacuac      120

auguaccuag accgcaauga ugccggcgaa gcaauaucgu uccccaccac ccucggcaug      180

aacaaaugcu acauacaaau cauggaccua ggacacaugu gcgacgccac cauguccuac      240

gaaugcccca ugcuagacga agggguugaa cccgaugacg ucgacugcug gugcaauaca      300

acgagcaccu gggugguuua cggaacgugc caccacaaga aaggggaggc ccgccgcucc      360

cgcagagccg uaacccuccc cucacacagc accagaaaac uacagacacg aagccagaca      420

uggcucgaau cacgagaaua caccaagcac cucauccgcg uagaaaacug gauauuccgc      480

aaccccggcu ucgcccuagc cgcagccgcc aucgccuggc uccuuggcuc cucaacaagc      540

caaaaaguca uauaccucgu caugauucua cucaucgcgc ccgccuacuc cauccguugc      600

aucggagucu ccaacagaga cuucgucgaa ggcaugucag gaggcaccug gguugacauc      660

guccuugaac acggggguug cguaacggug auggcccagg acaaacccac aguagacaua      720

gaacuuguga ccacaacugu auccaacaug gcggaagucc gcucauacug cuacgaagcc      780

agcaucucgg acauggccuc cgacucccga ugcccaacac aaggcgaagc auaccucgac      840

aaacaauccg acacccaaua cgucugcaaa cggacacucg ucgaccgcgg auggggaaac      900

ggcugugguc uauucggcaa aggaucccua gugaccugcg caaaauuugc uugcucaaag      960

aaaaugacgg gaaaaucaau ccagcccgaa aaccucgaau acagaaucau gcuuuccguc     1020

cacggcagcc aacacagcgg aaugauaguc aacgacaccg gccaugaaac cgacgaaaac     1080

cgggccaaag uagaaaucac cccaaacuca ccccgcgcag aagccacccu cggaggguuc     1140

ggaagccucg gacucgacug cgaaccacgc acuggacuag acuucucaga ccuguacuau     1200

cucacgauga acaauaaaca uuggcuagua cacaaagagu gguuccacga uauaccacua     1260

ccauggcacg ccggagcaga cacaggcaca ccccacugga acaacaaaga agcccucgua     1320

gaguucaaag acgcacacgc caaacgucaa accgucguag uacucgguuc acaagagggc     1380

gcuguacaca ccgcacuagc cggcgcccuc gaagccgaaa uggauggagc caaagggcgc     1440

cuauccucag gccaccuaaa augccgacuc aaaauggaca aacuucgccu caaaggggua     1500

ucauacuccc uaugcacagc cgccuucacc uucacaaaga uacccgccga aacauuacac     1560

ggcaccguca cagucgaggu ccaauacgcc ggcaccgacg gacccugcaa agucccggca     1620

cagauggcag uagacaugca aacccucaca cccgucggcc gccuaauaac agcaaacccc     1680

gucaucaccg aaucaaccga aaacagcaaa augaugcugg aacucgaccc acccuucggc     1740

gacuccuaca ucguaaucgg cguaggcgaa aagaaaauaa cccaucacug gcacaggucc     1800

gguagcacca ucggaaaagc auucgaagca accguccgag gcgccaaacg gauggcugua     1860

cuaggagaca cugccuggga cuucggcucc guuggcggcg cccuaaacuc gcuaggcaaa     1920

ggaauccacc aaaucuucgg cgccgccuuu aaaagccuau ucggaggaau gagcugguuc     1980

ucacaaaucc ucaucggaac acuacuaaug uggcugggac ugaacaccaa aaacggaagc     2040

auaucacuua ugugccucgc acuagguggc guucuuaucu uccucagcac agcgguaucu     2100

gcaugaggac uaguuauaag acugacuagc ccgaugggcc ucccaacggg cccuccuccc     2160

cuccuugcac cgagauuaau agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2280

ccccccaaag gcucuuuuca gagccaccag aauu                                 2314


<210>  9456
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9456
gggagaaagc uuaccaugaa gaaacggcga ggcgccgaca caagcgucgg caucguaggc       60

cuacuccuaa ccaccgcuau ggcagccgaa guaacacgcc gaggaagcgc auacuauaug      120

uaccuagauc gcaacgacgc aggcgaggcc aucagcuucc ccaccacauu aggcaugaau      180

aaaugcuaca uccaaauaau ggaucuaggc cacaugugcg augccacaau gaguuacgaa      240

ugcccaaugc ucgacgaggg agucgaaccc gacgacguag acugcuggug caacaccacu      300

ucaacauggg ucguauacgg aaccugccac cacaagaaag gcgaagcaag gagaucaagg      360

agagcuguca cgcuccccuc acacagcacc cgcaaacuac aaacacgguc ccaaaccugg      420

cuggaaagcc gagaguauac aaaacaccua auccgcgucg aaaauuggau auucagaaac      480

cccggcuucg cgcucgcagc agccgcaaua gcauggcuac ucggcucaag cacuucacaa      540

aaagucaucu aucuagucau gauccuucuc aucgcaccag cauacaguau acgcugcaua      600

gggguaagca accgggacuu cguugaagga auguccgggg gcacgugggu agacauagua      660

cuagaacacg gaggaugcgu aacgguaaug gcccaagaca aacccacagu ggacaucgag      720

cuaguaacga caacagucag caacauggcc gagguacggu cauacugcua cgaggcaagc      780

auaucagaca uggcauccga cucaaggugc cccacacagg gcgaggccua ccucgacaag      840

caaagcgaca cacaauacgu cugcaaacgg acccucguag auagaggcug gggcaacgga      900

ugcggccucu ucggaaaagg gagccuagua acaugcgcaa aauucgccug cagcaagaaa      960

augaccggaa aaagcauaca accagaaaac cuugaauacc gcauaaugcu aagcguacac     1020

ggcagccaac acagcgguau gaucguaaac gacacaggac acgaaaccga cgaaaauaga     1080

gcaaaaguag aaauaacacc gaacuccccu cgggcagaag ccacacucgg gggauucgga     1140

agccuaggcc uagacugcga gcccagaacc ggacuggacu ucagcgaccu cuacuaccua     1200

acaaugaaca acaagcacug gcuaguacac aaagaauggu uccacgacau accucuccca     1260

uggcacgcag gggcagacac aggcacaccc cacuggaaca auaaagaagc ccuaguggag     1320

uucaaagacg cccacgcaaa aagacaaacc guaguagucc ucggaagcca agaaggcgcc     1380

guccacaccg cgcuagccgg ggcgcuggaa gcggaaaugg acggcgccaa aggacgacua     1440

agcagcggcc accuaaaaug ccgacuaaaa auggacaaac uaaggcucaa aggcguaucc     1500

uacucacucu gcacagccgc cuucaccuuc acaaaaauac cagcagagac gcuccacggc     1560

acagugacgg uggaagucca auaugccggc acagacggac caugcaaggu gccagcacaa     1620

auggcggucg acaugcaaac acuaacaccc guaggccgcc uaauaaccgc caaccccgua     1680

aucaccgaaa gcacggaaaa uucgaaaaug augcuagagc ucgacccacc guucggagac     1740

ucauacaucg ucauaggagu gggcgagaaa aagauaacac accacuggca ccgaagcggc     1800

uccacaaucg gaaaagcauu cgaagccaca guaagagggg ccaagagaau ggccguacuc     1860

ggagauacag caugggauuu cgggucagua ggcggagccc uaaacagccu aggcaaaggc     1920

auccaccaga ucuucggagc agcauucaaa agucucuuug gaggaauguc cugguucucc     1980

caaauacuga ucggcacacu acugaugugg cugggacuaa acaccaaaaa cgggagcauc     2040

ucacucaugu gucuggcacu gggcggagua cucaucuucc uaagcacagc cguaucagca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  9457
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9457
gggagaaagc uuaccaugaa aagacgcggc gcggacaccu ccgucggcau agucggucuu       60

cugcucacaa cagcaauggc cgcagagguc acacgaagag gcagcgccua cuacauguac      120

cucgaccgaa acgacgcagg cgaagcaauc ucauucccca caacccuagg caugaacaaa      180

ugcuacaucc agauaaugga ccuaggccac augugcgacg ccacaauguc cuacgaaugc      240

ccuauguuag acgaaggagu ugaaccagac gacguagacu gcuggugcaa cacaacauca      300

acgugggugg uguauggcac augccaucac aagaaaggag aagccagacg cagcagacga      360

gcagucaccc uacccagcca cucaacacga aaacuccaaa cacggucaca gacauggcuc      420

gaaagccgcg aauacacgaa acaccuaauc cgggucgaga auuggaucuu ccgcaaucca      480

ggauucgccc ucgcagccgc agccaucgcc uggcuacuag gaagcuccac cucacaaaaa      540

gucaucuacc ucgucaugau acuccuaaua gcaccagccu acuccaucag augcauaggc      600

gucagcaacc gcgacuucgu agaaggaaug aguggcggca ccugggucga caucguccua      660

gaacacggcg gaugcguaac cgugauggcg caggacaaac ccaccgugga cauagagcua      720

gucaccacca ccgucucgaa cauggcagaa guacgauccu acugcuacga agccagcaua      780

agcgacaugg caagcgacag ucgcugcccc acccaaggag aagccuaccu agauaaacaa      840

agcgacaccc aauacgucug uaaacgaacc cuagucgacc gcggaugggg caacggcugu      900

ggacucuucg ggaaaggcuc acuggugaca ugcgccaaau ucgcgugcuc uaagaaaaug      960

acagggaaau cuauccaacc cgaaaaccua gaauaccgca ucaugcuaag cguacacggc     1020

ucacaacacu ccggcaugau aguaaacgac acaggacacg aaaccgacga gaacagagcc     1080

aaaguagaaa ucacgcccaa cucccccagg gccgaagcaa cccucggcgg cuucggaucc     1140

cuugggcuag acugcgaacc cagaaccgga cucgacuuca gcgaccucua cuaccuaaca     1200

augaacaaca aacacugguu agugcacaag gaaugguucc acgacauccc acuaccaugg     1260

cacgcgggag cagacacugg cacaccccac uggaacaaca aagaagcucu cgucgaguuc     1320

aaggacgccc acgccaaaag gcaaacaguc gucguccuag ggagccagga aggagccgug     1380

cacaccgcac ucgcuggcgc ccuggaagcc gaaauggacg gagccaaagg cagacuauca     1440

agcggacacu uaaaaugcag acuaaaaaug gauaaacucc gucuaaaagg cguaagcuac     1500

agccuaugca ccgccgcguu cacauucacc aaaauaccag cagaaacucu ccacggcacc     1560

gugacaguag aaguccagua cgccggcacg gauggaccau guaaaguacc ggcccaaaug     1620

gcaguagaca ugcaaacacu cacacccgua ggacgccuaa ucaccgcaaa ccccgugaua     1680

accgaaucaa ccgaaaacag caaaaugaug cucgaacucg acccaccauu uggagauagc     1740

uacaucguga ucgggguagg agagaaaaag aucacgcacc auuggcaccg cucaggcuca     1800

accauaggaa aggccuucga agcaaccgua cgaggagcaa aacgcauggc aguacuagga     1860

gacacugccu gggacuucgg cucaguaggc ggagcacuca acucccuagg caaaggaaua     1920

caccaaaucu ucggcgccgc cuucaaaagc cucuucggcg guauguccug guucagccaa     1980

auccugaucg gcacacuucu aaugugguua ggacucaaca ccaaaaacgg cuccaucucc     2040

cuaaugugcc uagcacucgg cggcguacuc aucuuccuaa gcacagcagu guccgcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  9458
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9458
gggagaaagc uuaccaugag acgaggcgcc gacacaucag uuggaauagu aggacuacua       60

cuaaccacag ccauggccgc agaaguaaca agacgcggau ccgccuauua cauguaccua      120

gaccgaaaug acgcaggcga ggcgaucagc uucccaacaa cgcugggaau gaacaaaugc      180

uacauacaaa uaauggaccu uggccacaug ugugacgcaa caaugagcua cgaaugcccc      240

augcucgacg agggcgucga accagacgac guagacugcu ggugcaauac aacaagcacc      300

uggguagucu acggaacaug ccaccacaaa aagggagaag cacgacgcag ccgccgagcg      360

guaacccucc ccagccauuc gacccggaaa cuacagaccc gaagccaaac cuggcuagaa      420

ucacgcgaau acaccaaaca cuuaaucaga guggagaacu ggaucuuccg caaccccgga      480

uucgcacuag cagcagccgc aauagcaugg cuccuaggca gcagcacaag ccaaaagguc      540

auauaccucg ugaugaucuu acucauagcc ccagccuaca gcauacgaug cauaggcgug      600

uccaacagag acuucgucga aggcaugagc ggaggcacau ggguagacau aguacucgaa      660

cacggcggau gcguaacggu gauggcccaa gacaaaccaa caguagauau cgaacugguc      720

accaccacag ucagcaauau ggcagaagua agauccuacu gcuacgaagc auccauaagc      780

gacauggcaa gcgauagcag augccccaca cagggcgaag ccuaccucga caaacaauca      840

gauacacaau acgucugcaa gcgcacccug gucgacagag gcuggggaaa uggaugcggc      900

cuauuuggaa aagggucccu ggucacgugc gcaaaguucg cgugcagcaa gaaaaugacc      960

ggcaaaagca uccaacccga gaaccucgaa uacagaauaa ugcucagcgu gcacgggagc     1020

caacacagcg gcaugauagu aaacgacacu ggacacgaaa ccgaugagaa ccgagccaaa     1080

gucgaaauaa ccccuaacuc accacgagca gaagcgacac uagggggcuu cggcagucuc     1140

gggcuagacu gcgagcccag aaccggccuc gacuuuuccg accucuacua ccuaaccaug     1200

aacaacaaac acuggcucgu gcacaaagaa ugguuccacg acaucccacu cccauggcac     1260

gcaggagcag acacaggaac accccacugg aacaacaaag aggcacucgu cgaguucaag     1320

gacgcccacg caaaaagaca aacaguagua guucucggaa gccaagaagg agcaguacac     1380

accgcccuug caggggcccu cgaagccgag auggacggag cgaaaggccg gcuaucguca     1440

ggccaccuca aauguagacu aaagauggac aaacucagac uaaaaggagu gucauacagc     1500

cugugcacag ccgcguucac auucacaaaa auaccggcgg aaacacucca cgggacgguc     1560

accguagagg ugcaauacgc cggcaccgac ggccccugca aagucccggc acagauggcc     1620

gucgacaugc aaacccucac accaguaggc cgacuaauca cagccaaccc gguaauaacg     1680

gaauccacag aaaacucaaa aaugaugcua gaacucgacc caccauuugg cgacagcuac     1740

auagugauag ggguaggcga aaagaaaauc acacaccacu ggcaccgaag cggcagcacc     1800

auagggaaag cauucgaagc aaccguacgg ggagcaaaac gcauggcagu acucggggac     1860

acagccuggg acuucggcag cguaggcggu gcacuaaacu cacuaggcaa gggcauacac     1920

caaauauucg gggcggccuu caaaucucua uucgggggca ugagcugguu cucacaaauc     1980

cuaaucggga cccuccucau guggcuagga cucaauacaa agaacggcag cauaucgcuc     2040

augugccugg cauuaggagg aguccuaauc uuccucagca cagcaguaag cgcaugagga     2100

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2160

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  9459
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9459
gggagaaagc uuaccaugcg cggagcagac accagcgucg gcaucguggg ccuccuucug       60

acaacagcca uggccgcgga gguaacacga agaggcagcg cguacuacau guaccuagac      120

agaaaugacg caggagaagc caucucguuc ccaacaaccc uaggaaugaa caaaugcuac      180

auccaaauca uggaccuagg ccacaugugc gacgccacca ugagcuacga augcccaaug      240

cucgacgaag gaguagagcc cgacgacgua gacugcuggu gcaacaccac cagcacaugg      300

guaguauacg gaaccugcca ccacaaaaag ggggaagcuc gccgaagccg ccgcgcuguc      360

acacucccca gccacagcac cagaaaacuc caaacccgcu cacaaaccug gcuagaaagc      420

cgagaauaca ccaaacacuu aauccgagug gaaaacugga uauucaggaa ucccggcuuc      480

gcccuugccg ccgccgcaau agccuggcuc cucggcagcu caaccucaca aaaagucaua      540

uaccuaguca ugauacuccu aauagccccc gccuacucca uaagaugcau aggcguaagc      600

aacagagacu ucguagaagg gaugucuggc ggcaccuggg uagacauugu acucgaacac      660

ggcggcugug ugacagugau ggcccaagac aaaccaaccg uagacaucga gcuagucaca      720

accaccgucu ccaacauggc cgaagucaga uccuacugcu acgaagcgag cauauccgac      780

auggcguccg acagccgaug ccccacacag ggcgaagccu accucgacaa acaaagcgac      840

acccaguacg uauguaaacg caccuuagug gacagaggau ggggcaacgg cugcggacuc      900

uucggcaagg gaucccucgu cacaugcgcc aaauucgcau gcucgaagaa aaugaccggc      960

aaaagcaucc aaccagaaaa ccucgaauac cgaauaaugc ucucgguaca cggcagccaa     1020

cacagcggaa ugaucguuaa cgacaccggg cacgagacag acgaaaaccg ugccaaaguc     1080

gaaauaaccc ccaacagccc aagagcagag gccacccucg gcggauucgg uagccuagga     1140

cuagacugcg agcccagaac cggccuggac uucucagacc ucuacuaccu aacuaugaac     1200

aacaaacacu ggcuagucca caaagaaugg uuccacgaca uaccccuacc cuggcacgcc     1260

ggagcagaca caggcacccc acacuggaac aacaaagaag cccuaguaga guucaaggac     1320

gcacacgcaa aacgccaaac aguuguaguc cuuggcagcc aagaaggagc cguccacacc     1380

gcccucgcag gagcccuaga agcagaaaug gauggcgcga aaggccgccu aucauccggc     1440

caccuaaaau gcagacucaa gauggacaaa cuaagacuca agggagucuc cuacucccua     1500

uguaccgcag ccuucacguu caccaaaauc ccagcagaaa cacuccacgg caccguaacc     1560

gucgaaguac aauaugccgg uacagacgga cccugcaagg uaccagccca aauggccgug     1620

gacaugcaaa cccuaacacc cguaggccgc cugaucacag cgaaccccgu uaucaccgaa     1680

uccacagaaa acucaaagau gaugcucgaa cuagacccac ccuucggaga cuccuacaua     1740

gucaucggcg ucggggaaaa gaaaauaaca caccacuggc accgcuccgg cagcaccaua     1800

gguaaagcau ucgaggccac cguacgagga gccaaacgaa uggccguacu aggcgacacc     1860

gccugggacu uuggauccgu gggcggcgca cuaaacucac uaggcaaagg aauacaccaa     1920

aucuucgggg cagccuucaa aagccuauuc ggcggaaugu cuugguucuc acaaauccuc     1980

auaggaacac uccugaugug gcucggacug aacaccaaaa acggauccau aagucuaaug     2040

ugccuagcac ugggcggagu ccuaaucuuc cugagcaccg caguauccgc gugaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9460
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9460
gggagaaagc uuaccauggg cgccgacaca ucggucggaa uagucggacu acuccucaca       60

acagccaugg cagcggaagu cacgcgaaga ggcuccgccu acuacaugua ccucgaccgc      120

aacgacgcag gggaagcgau cagcuucccc accacccucg ggaugaacaa augcuacaua      180

caaauaaugg aucuaggaca caugugcgac gcgacgauga gcuacgaaug ccccaugcua      240

gacgaaggag uagaaccaga cgacgucgac uguuggugca acacaacauc cacuugggug      300

guauauggaa ccugccauca caagaaagga gaagccagga ggucccgcag agccgucaca      360

cuccccagcc acuccacucg caagcuacaa acaagaucac aaaccuggcu agaaagcagg      420

gaauauacaa aacaccuaau aagaguggaa aacuggaucu ucagaaaccc cggcuucgcc      480

cuagccgcag cggccauagc cuggcuacuc ggcagcucca caagccagaa agucaucuac      540

cucguaauga uccuucugau cgccccagcg uacuccauac gguguauagg aguauccaac      600

cgcgacuucg ucgaaggcau gucaggcgga acaugggucg acauaguccu cgaacacggu      660

ggaugcguca cgguaauggc acaggacaaa cccacggucg auaucgagcu aguaacaacc      720

acggucucca acauggccga aguccgcuca uacugcuacg aagccuccau auccgacaug      780

gcguccgacu cccgcugccc aacccaaggc gaagccuacc ucgacaaaca auccgacacc      840

caauacguau gcaaacgaac cuuagucgac cgcggauggg ggaacggaug uggccuauuc      900

ggcaagggcu cgcucguuac augcgccaaa uuugccugcu cgaagaaaau gacgggaaaa      960

agcauccaac ccgaaaaccu agaauacaga auaaugcuau cgguccaugg aucacaacac     1020

ucagggauga uaguaaacga cacgggacac gagaccgacg aaaaccgcgc gaaagucgaa     1080

auuaccccaa acaguccucg cgcagaagca acccuaggcg gguucggaag ccucggccuc     1140

gacugcgaac cacggacagg cuuagacuuu agcgaccugu acuaccucac caugaacaac     1200

aaacacuggu uaguccacaa agaaugguuc cacgacauac cccuaccaug gcacgccgga     1260

gccgauacag guacacccca cuggaacaac aaggaagcgc uaguggaguu caaagacgcc     1320

cacgcaaaac gccaaaccgu cgucgucuua ggcagccaag aaggagccgu ccacaccgcg     1380

cucgccggag cccuagaagc agagauggac ggagcaaaag gacgccuaag uucagggcac     1440

cuaaagugcc gacugaaaau ggacaagcug cgucucaaag gcguguccua cucacuaugc     1500

acggcggcau uuacguucac caaaaucccc gcagaaaccc uacacggaac cgucaccgua     1560

gaaguccaau augccggaac ggacggaccc ugcaaggucc cagcccaaau ggccgucgac     1620

augcagaccc ugacccccgu aggccggcua auaaccgcaa acccgguaau aacagaauca     1680

acagagaacu caaaaaugau gcucgaacug gacccacccu ucggcgacuc cuacauaguc     1740

aucggaguag gagaaaagaa aauaacccac cacuggcacc gcagcggaag caccauaggc     1800

aaagcguucg aggcgaccgu ccgcggcgcc aaaaggaugg caguccuagg agacaccgca     1860

ugggacuucg gaagcguagg aggcgcccuc aacucacuag gaaaaggaau ccaccaaauc     1920

uucggagcgg cguucaaaag ccucuucggc ggaaugagcu gguucucaca aauccucauc     1980

ggaacccugc uaauguggcu cggacuaaac accaaaaacg ggagcaucuc ccuaaugugu     2040

cucgcacuag guggcguccu caucuuccuc agcacagcag uaucagcgug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  9461
<211>  2296
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9461
gggagaaagc uuaccauggc cgacacaagc guaggaauag uaggacugcu ccugaccaca       60

gcaauggcag cagaaguaac aagacgaggc uccgcguauu acauguacuu agaccgaaac      120

gacgccggag aagccauauc uuuccccacc acccuaggga ugaacaagug cuacauucaa      180

auaauggacu ugggccacau gugcgaugcg accaugagcu acgagugccc caugcuagac      240

gagggcgucg aaccagacga cguagacugc uggugcaaca cgaccucaac auggguagua      300

uacgggacau gccaccacaa gaaaggagag gcacgaagaa gccgacgagc cguaacgcua      360

cccucccacu cgacacgcaa acuccaaaca agaucccaaa cauggcugga aagcagggaa      420

uacacaaaac accucauuag aguagaaaac uggauauuuc gaaacccagg cuucgcccua      480

gcagcagcag cgaucgccug gcuccucgga agcagcaccu cgcaaaaagu aauuuaccuc      540

guaaugauac uacuaaucgc gccagcauac agcaucaggu guaucggagu aagcaacaga      600

gacuucguag aaggcauguc aggaggaacc uggguagaca ucguacuaga acacggagga      660

ugcguaaccg ugauggcuca agacaaacca accguagaca ucgagcuagu aaccacuaca      720

gucuccaaua uggcugaagu cagauccuac ugcuacgaag ccucaauuag cgacauggcg      780

agcgacagcc gaugcccgac ccaaggagaa gccuaccucg acaaacaauc ggacacccaa      840

uacguaugca aaaggacacu ggucgaccga ggcuggggga acggcugcgg ccuguucggg      900

aaaggcagcc ucguaacaug cgcgaaauuc gccugcucca agaaaaugac uggaaaauca      960

auccaaccag aaaaccugga auacagaauc augcucuccg uacacgggag ccaacacagc     1020

ggaaugaucg uaaacgacac cggccacgaa accgacgaaa accgcgcgaa agucgaaaua     1080

acgcccaacu cacccagagc cgaggccaca cuaggagguu ucggaucacu agggcucgac     1140

ugcgaaccca ggaccggacu agacuucagc gaccucuacu accucacaau gaacaacaaa     1200

cacuggcuag ugcacaaaga augguuucac gacauacccc uacccuggca cgcaggcgca     1260

gacaccggca cgccccacug gaacaacaaa gaagcacucg ucgaguucaa agacgcccac     1320

gccaaacgcc agaccguagu cguccuagga agccaagaag gagcagucca caccgcccua     1380

gcaggggcuc uagaagccga aauggacggc gcaaagggac gacucagcag cggccaccua     1440

aaaugccgcc ugaaaaugga caaacucaga cucaaaggag uaucauacuc acuaugcaca     1500

gcagcauuca cauucaccaa aauccccgca gaaacccucc auggaacagu cacagucgaa     1560

guucaauaug ccggcacaga cggaccaugc aaaguacccg cacaaauggc cguagacaug     1620

cagacgcuaa cccccgucgg aaggcucaua acagcuaacc ccgucauaac cgaaagcaca     1680

gaaaacucca aaaugaugcu cgaacucgau cccccguucg gcgacucgua cauugucaua     1740

ggcgucggag aaaagaaaau cacacaccac uggcaccgcu caggcagcac aaucggcaaa     1800

gccuucgaag cgacaguacg gggagccaaa cgaauggccg uccucggaga cacagccugg     1860

gacuucggaa gcgucggagg agcccuuaac agucugggaa aaggaaucca ccaaaucuuc     1920

ggggcagcuu ucaaaagccu cuucggcggc augagcuggu ucagccaaau ccuaaucgga     1980

accuuacuaa uguggcucgg gcugaacacg aaaaacgggu ccauaagccu caugugccuc     2040

gcccugggcg gaguccucau auuccuaagc acagccgucu ccgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2280

cagagccacc agaauu                                                     2296


<210>  9462
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9462
gggagaaagc uuaccaugga cacaagcguc ggaauagugg gucuccuacu cacgaccgca       60

auggccgcag agguaacccg aaggggcucu gcauacuaca uguaccugga ccgcaacgac      120

gcaggcgaag ccauaucauu ccccacgaca cucggaauga acaaaugcua uauacagauc      180

auggaccucg gacauaugug cgacgccacc auguccuacg aaugccccau gcuagacgaa      240

ggcguggaac cagacgacgu cgacugcugg uguaacacca cgagcaccug gguagucuac      300

ggcacaugcc accacaagaa aggggaagcc agaagaucac gccgagcggu aacgcucccg      360

ucacacucca caagaaaacu acagacacga agccaaaccu ggcucgaaag ccgcgaauac      420

accaagcacc uaauaagagu agagaacugg auuuuccgaa acccuggguu cgcgcuagca      480

gcugcagcaa uagccuggcu ccuagggagc ucaacgucac agaaagucau cuaccucguc      540

augauacuac ugaucgcacc cgccuacucc auccgaugca uaggcgucuc aaaccgcgac      600

uucguagaag gcauguccgg agggaccugg gucgauaucg uucucgaaca cggaggaugc      660

guaacaguca uggcccagga uaaacccaca guagacauag aacucguaac aacgacaguc      720

ucaaacaugg ccgaaguaag guccuacugc uacgaagcau caaucucgga cauggccucc      780

gacucacgau gccccaccca aggcgaagcc uaccucgaca aacaaucaga cacccaauau      840

gucuguaaac ggacccucgu agaccgcggc uggggcaacg gaugcggccu auucggcaaa      900

ggcucccuag ugaccugcgc caaauucgca ugcucaaaga aaaugacagg caaaagcaua      960

caacccgaaa acuuagaaua ccguaucaug cuuuccguac acggcagcca acacuccggc     1020

augaucguca augacaccgg ccacgaaacu gacgaaaaua gagcaaaagu agaaaucaca     1080

ccaaacagcc cacgagcgga agcgacgcuc ggcggcuuug gcucacucgg ccucgacugu     1140

gagccaagaa caggacuaga cuucuccgau cuauacuacc ucacgaugaa caacaaacau     1200

uggcuaguac acaaagaaug guuucaugau aucccccuac cuuggcacgc cggagccgac     1260

acaggaacac cucacuggaa caauaaagag gcacucguag aguucaaaga cgcacacgcc     1320

aaaagacaaa ccguagucgu ccuuggcuca caagaaggcg ccguacacac cgcccucgcc     1380

ggagcacucg aagccgagau ggacggcgcc aaaggccgac uaucuagcgg ccaccucaag     1440

ugcaggcuca agauggauaa acuacgacuc aagggaguau cauacucccu augcaccgca     1500

gcauucacgu ucacaaaaau acccgcagaa acccuacacg gcacagucac aguggaggua     1560

caguacgccg gcacagacgg accauguaaa gucccagcac agauggccgu ugacaugcag     1620

acccuaaccc ccgucgggag acucaucaca gcaaacccag uaaucacaga aagcacagaa     1680

aacucaaaga ugaugcucga acuugaccca cccuucggcg acuccuacau cgucauagga     1740

guaggagaga agaaaauaac ccaccacugg caccgcuccg gaaguacaau aggcaaagca     1800

uucgaagcca ccguccgagg cgcaaaacgc auggcggucc uaggcgacac cgcgugggac     1860

uucggaagcg ucggcggcgc acucaacucu cugggaaaag gcauacacca gauuuucggc     1920

gcagccuuca aaucccuauu cggcggcaug agcugguuca gccaaauacu caucggcacu     1980

cuccuaaugu ggcuaggccu caacacgaaa aacggcagca uaagccuaau gugccucgcc     2040

cuaggaggag uccuaauauu ccuaucaaca gcagugagcg cuugaggacu aguuauaaga     2100

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2160

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  9463
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) Polyprotein(108-794) mRNA Design 3 opt

<400>  9463
gggagaaagc uuaccaugac cagcgucggg aucgucggcc uccuacuaac cacagcaaug       60

gcagcagaag uaaccaggcg cgguuccgcc uacuauaugu accucgacag aaacgacgcg      120

ggcgaagcca ucuccuuucc aaccacacuc ggcaugaaca aaugcuacau ccaaauaaug      180

gaccucggac acaugugcga cgccacgaug uccuacgaau gucccaugcu agacgaaggu      240

gucgaaccag acgacguaga cugcuggugc aacaccacca gcaccugggu gguauacggc      300

acaugccacc acaagaaagg cgaagccaga cgaucgcgcc gagccgucac gcuccccucc      360

cacucgacca gaaaacuaca aacccgcucu cagaccuggc ucgaaucgcg agaauauacc      420

aaacaccuua uacgagucga aaacuggaua uuccgcaacc caggauuugc auuagccgcc      480

gcagcaaucg ccuggcuacu cgggagcuca acaucacaaa aaguaauuua ccucgucaug      540

auccugcuca uugccccagc uuacucaaua cgauguaucg ggguaagcaa ccgagacuuu      600

gucgaaggca ugagcggagg gaccuggguc gacaucgucc ucgaacacgg cggcugcgua      660

acagucaugg cccaggauaa acccaccguc gacauugagc uagucacaac cacagucucg      720

aacauggccg aaguacgcag cuacugcuac gaagcgucca uaagugacau ggccagcgac      780

ucccgcugcc ccacucaagg cgaagccuac cucgauaaac aaagcgacac ccaauacgua      840

ugcaaaagaa cccucgucga ccgaggcugg ggcaacgguu gcggccuauu cggaaaaggc      900

ucccuaguua ccugcgcaaa guucgcaugc ucaaagaaaa ugaccggcaa auccauccaa      960

ccagaaaacc uggaauaccg cauaaugcug agcguccacg gcucccaaca cuccggaaug     1020

aucgucaacg acacagggca cgaaacggac gaaaaccgcg caaaaguuga aauaaccccc     1080

aauagccccc gcgcagaagc aacacucggc ggcuucggaa gccuagggcu cgacugcgaa     1140

ccccgcacag gccucgacuu cuccgaccug uacuaccuaa ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacauc ccacuaccau ggcacgcggg ugcagacaca     1260

ggcacacccc acuggaauaa caaagaagcc cucguagagu ucaaagaugc ccacgcaaaa     1320

agacagaccg ucguaguccu cggcagucaa gaaggcgccg uccacacggc ucucgccgga     1380

gcccuagaag ccgaaaugga cggagcaaaa ggaaggcuau ccucaggcca ccucaaaugc     1440

aggcucaaga uggacaaacu aagacucaaa ggcguaagcu acagccucug cacagccgca     1500

uucaccuuca cgaaaauucc cgcggaaacg cuacacggca cagugacagu cgaaguccag     1560

uacgcaggca cagacgggcc augcaaagua cccgcacaaa uggcggucga caugcagaca     1620

cucacccccg uaggaagacu uaucacagca aaccccguaa ucacagaauc cacagaaaac     1680

agcaaaauga ugcuagaacu agauccaccc uucggagacu ccuacaucgu aauaggcguc     1740

ggagaaaaga aaaucacuca ccauuggcac agaagcggcu ccacaauagg gaaagccuuc     1800

gaggcgacag uacgcggagc uaaacgcaug gccguacucg gggacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcacu aaacagucuc ggcaaaggaa uacaccaaau cuucggcgcc     1920

gccuucaaau cccuauucgg aggcaugagc ugguucagcc aaauccugau aggcacauug     1980

cucauguggu uaggccucaa caccaaaaac ggcagcauca gccucaugug ccucgcacuc     2040

gggggaguac uaauauuccu auccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9464
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9464
gggagaaagc uuaccaugac cuccguaggc aucgugggac uccuccucac aaccgcaaug       60

gccgcagccg uaaccagacg cggcuccgca uauuacaugu accucgacag aaacgacgcc      120

ggcgaagcga ucucguuucc caccacccuc gggaugaaca aauguuacau ccaaaucaug      180

gaccucggcc acaugugcga cgccaccaug agcuacgaau gccccaugcu agaugaagga      240

guagaaccag acgacguaga cugcuggugc aacaccacau caacaugggu aguguacggc      300

acaugucacc acaagaaagg ugaagcacgg cgcagcagac gcgcgguaac gcuccccuca      360

cacuccacac gcaaacucca aaccagaagc caaaccuggc uagaaagccg agaauacacc      420

aaacaccuca uacgagucga aaacuggaua uucagaaacc ccggauucgc ccucgcggcc      480

gcggcaauag cuuggcuacu cggauccucg accucacaaa aagucaucua ccuggucaug      540

aucuuacuca uagcaccagc auacucgauc cgguguaucg gcgugucuaa ccgcgacuuu      600

gucgagggca uguccggcgg caccugggug gacaucgucc uagaacacgg gggcugcguc      660

accguuaugg cccaagacaa acccacggua gacauagagc ucgucaccac cacaguaucc      720

aacauggccg aaguccgauc auacugcuac gaagccucca uaagcgacau ggccucagac      780

ucccgcugcc ccacacaagg ugaagcauac cuagacaaac aaucagacac gcaauacgug      840

ugcaaacgca cucuggucga ccgaggcugg ggcaacggcu gcggccucuu cggcaaggga      900

agccuaguaa ccugcgccaa auucgccugc ucaaagaaaa ugaccggcaa aucaauccaa      960

cccgaaaacc ucgaauaccg aaucaugcua ucaguccacg gaucacagca cucuggcaug     1020

auagucaacg acacagguca cgaaacagac gaaaaccgcg ccaaaguuga aaucaccccc     1080

aacucaccuc gagcagaagc cacccucgga ggauucggcu cccuaggccu agacugcgaa     1140

ccacgcacag gacuagacuu cagcgaccuc uauuaccuca ccaugaacaa caaacauugg     1200

cuaguucaca aagaaugguu ccacgacaua ccccucccau ggcaugccgg ugcagacacc     1260

ggaacaccuc acuggaacaa caaggaagcc cucguagagu ucaaggacgc acacgcaaaa     1320

cgacaaacag ucguaguccu cggaagccag gaaggagccg uucacaccgc acuugcagga     1380

gcccuggaag ccgaaaugga cggugccaaa ggccgccuuu ccucagggca ccucaaaugc     1440

cgccucaaaa uggacaaacu acgccucaaa ggcgucucuu acucccucug uacugccgcc     1500

uuuaccuuca ccaagauacc agcagaaacu cugcacggca ccgucacagu cgaaguacaa     1560

uacgcuggca ccgacggacc cugcaaaguc cccgcccaaa uggccgucga caugcagacc     1620

cuaacccccg uaggaagacu aaucacagcc aauccaguca ucaccgaauc cacagaaaac     1680

ucaaaaauga ugcucgaacu cgauccaccc uucggagaca gcuacauugu caucggcguc     1740

ggugaaaaga aaauaacaca ccacuggcau cgaucaggcu caacgaucgg aaaagcguuc     1800

gaagcuacag uacgaggcgc caaacggaug gccguccucg gcgacaccgc uugggacuuc     1860

ggcuccgucg gaggugcacu caacucacuc ggcaaaggua uccaccaaau cuucggcgca     1920

gcguucaaga gccucuucgg cggcauguca ugguuuucac aaauacucau aggaacacua     1980

cucauguggc ucgggcucaa cacaaagaac ggcagcauaa gccucaugug ccucgcacuu     2040

ggcggcgucc uaaucuuccu aucgacagca guguccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9465
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9465
gggagaaagc uuaccaugcg cggcgcggac acaucagucg gcaucgucgg gcuacuacuc       60

acgacggcca uggcagccgc ugucacaaga cgaggaagug ccuauuacau guaccucgac      120

cgcaacgacg ccggggaagc caucuccuuc cccacaacac ucggcaugaa caaaugcuac      180

auccaaauca uggaccucgg ccauaugugc gacgccacca ugucauacga gugcccaaug      240

cuagacgaag gagucgagcc agacgacguc gacugcuggu guaacacaac cuccacaugg      300

guaguuuaug gcacguguca ccacaaaaag ggcgaagccc gccgaagccg cagagccguc      360

acccuaccuu cacacagcac ccgcaagcua caaacacgca gccaaaccug gcuggaauca      420

cgagaauaca caaagcaccu aauacgcguc gagaacugga uauuccgcaa ccccggcuuc      480

gcacuagcgg ccgcagcgau cgcauggcuc cucggcucca gcacaucgca aaaggucauc      540

uaccucguaa ugauacuccu aaucgcaccc gcguauagca uacgguguau aggcgucagc      600

aaccgagacu ucguagaagg caugagcggu gguacauggg ucgacaucgu ccucgaacac      660

ggaggcugcg ucacagucau ggcacaagac aagccaacag uagacaucga acuuguaacc      720

accaccguua gcaacauggc ugaaguuaga ucauacugcu acgaagccag cauaagcgac      780

auggccucag acagccgaug cccaacgcaa ggagaagccu accuagacaa acaaucggac      840

acccaauacg ucugcaaacg aacccuaguc gaccgcggcu ggggaaaugg augcggccua      900

uucggaaaag gaucccucgu cacaugcgcc aaauucgcgu gcucaaagaa aaugaccggc      960

aaaagcauac aaccagaaaa ccuagaauac agaauaaugc ucucagucca cggcagccaa     1020

cacagcggca ugaucgucaa cgacacaggc cacgaaaccg acgaaaacag agcaaaaguc     1080

gagauuacac cuaacagucc acgcgcugaa gccacccuag gcggauucgg aucgcuaggc     1140

cucgacugcg aaccacguac cggacuagac uucuccgacc uauacuaccu cacaaugaac     1200

aacaaacacu ggcucguaca caaagaaugg uuccacgaca uaccccuacc cuggcacgcc     1260

ggagcagaca ccgggacacc acacuggaac aacaaagaag cccucgucga guucaaagac     1320

gcccacgcaa aacgccaaac agucgucgua uugggcuccc aagaaggggc aguccacaca     1380

gcccucgcgg gagcccuaga agcagaaaug gacggcgcca aaggacgacu aagcuccggc     1440

caccuuaagu gcagacucaa aauggacaaa cugcgacuua aaggagucuc auacucacuc     1500

ugcacagcag cuuucacauu cacaaaaaua ccagcagaaa cccuucacgg cacagugacg     1560

guagaagucc aguacgccgg aaccgacggc cccugcaaag uacccgccca aauggccgua     1620

gacaugcaaa cgcuaacccc aguuggacgu cucauaacag cuaacccagu aaucaccgaa     1680

ucgacugaaa acucgaagau gaugcuagaa cucgacccac cauucggcga cagcuacauc     1740

guaaucggcg ucggcgagaa aaagauaacu caucacuggc accgcagcgg uagcacaauc     1800

ggcaaagccu ucgaagccac gguaagggga gcaaaacgca uggcaguccu gggggacacc     1860

gcaugggauu ucggaucggu gggcggggcc cuaaacucac ugggcaaagg aauccaccag     1920

aucuucggcg ccgcuuucaa aucacucuuc ggcggcaugu ccugguucuc acaaauacuc     1980

aucggaacac uacucaugug gcucggacuc aacaccaaaa acggaagcau aagccucaug     2040

ugccucgcac uaggaggagu ccucauuuuc cucucaaccg ccguuuccgc augaggacua     2100

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     2160

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  9466
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9466
gggagaaagc uuaccaugcg cggccugagc agcagaaaac gacgaucaca cgacguacuc       60

acaguccaau uccucauacu cggaaugcua cucaugaccg gcggggucac gagaagaggc      120

agcgcauacu acauguauuu agaccgaaac gacgccggcg aagccaucuc cuuccccacc      180

acacugggca ugaacaaaug cuacauccaa aucauggacc uaggacacau gugcgacgcg      240

accaugucau acgaaugccc gaugcucgac gaaggcgucg aaccagacga cgucgauugc      300

uggugcaaca ccacauccac cuggguagua uacggcacau gccaccacaa gaaaggagaa      360

gcccgacgca gccggcgcgc ugucacccuc ccgucacacu cuacccgaaa gcugcaaacc      420

cgaucacaga ccuggcugga aucacgcgaa uacacaaaac accucauccg cgucgaaaac      480

uggauauucc gcaaccccgg cuucgcccua gcagcugcag ccaucgccug gcuacucggc      540

uccagcacca gccaaaaggu caucuaccuc guaaugaucc uccucauagc cccagcauac      600

ucaauccgau gcaucggagu gagcaaccgu gacuucgucg aaggaauguc cggcgggaca      660

ugggucgaua uugugcucga acacggaggc ugcgucacag uaauggccca agacaaacca      720

accguagaca uagagcucgu uaccaccacc guauccaaca uggccgaagu gcgcuccuac      780

ugcuacgaag caagcaucag cgacauggcc uccgauucaa gaugccccac gcaaggcgaa      840

gcauaccuag acaaacaauc agacacacaa uacgucugca aacgaacccu cgucgacagg      900

ggauggggga acggcugcgg ccuuuuuggg aaaggauccc uagucaccug ugccaaauuu      960

gcaugcagca aaaagaugac cggaaaauca auucaaccag aaaaccucga auaccgaaua     1020

augcucucag uccacggcag ccagcacagc ggaaugaucg uaaacgauac aggccacgaa     1080

acagacgaaa accgcgccaa aguagaaaua acacccaacu caccccgcgc agaagccaca     1140

cuagguggau ucgggagccu aggacuagac ugcgaacccc gcacaggucu agacuucucc     1200

gaccucuacu accucacaau gaacaacaag cacuggcuag uccacaaaga augguuccac     1260

gacaucccgc uaccauggca cgccggcgcc gacaccggaa caccccacug gaacaacaaa     1320

gaagcccuag ucgaguucaa agacgcacac gcaaagagac aaacugucgu aguccucggc     1380

ucccaagaag gcgccgucca uaccgcacuc gcaggcgcau uagaagccga gauggacggc     1440

gcaaaaggac gacuaagcag cggacaccuc aaaugccgcc uaaaaaugga caaacugaga     1500

cucaaaggag ucucuuacuc acucugcacc gccgcauuca ccuucacgaa aauccccgcc     1560

gaaacccucc acgguaccgu uaccgucgaa guccaauaug cagggacaga cggcccaugc     1620

aaaguccccg cccaaauggc agucgacaug cagacccuca cgccagucgg cagacuaauc     1680

acagcaaacc ccgucauaac cgaaagcacg gaaaacucca aaaugaugcu cgaacuagau     1740

cccccauucg gcgacucaua caucgucauc ggaguaggug agaaaaagau cacccaccac     1800

uggcacagaa gcggaagcac gaucgguaaa gccuucgaag ccacuguccg aggugccaaa     1860

cgcauggcug ugcucggaga caccgccugg gacuucggga gcgugggcgg agcccuaaac     1920

agccuaggga aaggcauaca ccagaucuuc ggcgcagcau ucaagagcuu auucggcggg     1980

augagcuggu ucucacaaau ccucaucgga acacuccuca uguggcuagg acucaacacc     2040

aaaaacggaa gcauaagucu gaugugccuc gcccucggag gaguccuuau auuccuaucc     2100

acagccguca gcgcgugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9467
<211>  2326
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV prME variant mRNA Design 3 opt

<400>  9467
gggagaaagc uuaccaugag aggccuauca agccgcaaaa gacggucaca cgacguauua       60

acaguacaau ucuuaauccu aggcaugcug cuaaugacag gggcaguuac ccgccgaggc      120

ucagcguacu acauguaccu ggaccguaac gacgccggcg aagcaauuag cuucccaacc      180

acacucggga ugaacaaaug cuacauccaa auaauggacc uaggacacau gugcgacgcc      240

acaaugucgu acgaaugucc aaugcuagac gaaggcgucg agccagacga cgucgacugc      300

uggugcaaua caaccagcac augggucgua uacggaaccu gccaccacaa gaaaggcgaa      360

gcccgccgau cccgccgcgc aguaacccuc cccagccaca gcacccgaaa acuccaaacc      420

cggagccaaa cauggcuaga gagcagggag uacacaaaac accugaucag gguagagaac      480

uggauauucc gaaaccccgg auucgcacuc gccgccgcag cuaucgccug gcuacucgga      540

uccuccaccu cucaaaaggu gaucuaucuu guaaugaucc uccucauagc acccgccuac      600

ucaauacgcu gcauaggagu uagcaacaga gacuucgucg aaggcaugag cggcggaaca      660

ugggucgaca ucguccucga gcacggcgga ugcgucacag ucauggccca ggacaaacca      720

accguagaca uagagcuagu aacaacaaca guauccaaua uggccgaagu ccgcuccuac      780

ugcuacgagg caucaaucuc agauauggcc ucagacucgc gauguccaac ccaaggggaa      840

gccuaccucg acaagcagag cgacacacag uacguaugua aaagaacccu uguugaccga      900

ggcuggggaa acggaugcgg ccuauuuggu aaaggaucac ucguaaccug ugccaaauuc      960

gcaugcucca agaaaaugac cggcaaaagc auacaacccg agaaccucga auaccgcaua     1020

augcucagcg uacacggcag ccaacacagc gggaugauag uaaacgacac cggacacgaa     1080

accgacgaaa aucgagcgaa aguagaaaua acaccaaaca gcccaagagc cgaagccacc     1140

cucggcggau ucggaagccu cggacuagac ugcgagccgc gcacagggcu cgacuucucc     1200

gaccucuacu accucacaau gaacaacaaa cacuggcucg uccacaaaga augguuccac     1260

gacauaccac uccccuggca cgccggcgcc gauaccggca ccccacacug gaacaacaaa     1320

gaggcccugg ucgaguucaa agacgcgcac gccaagcgcc aaacaguggu cguacucgga     1380

ucacaagaag gcgcaguaca cacagcccuc gccggagccc ucgaagccga aauggacggc     1440

gccaaaggac gccuaagcuc cggccaccuc aagugccgcc ucaaaaugga caaauugcga     1500

cucaagggag ucagcuacuc acugugcaca gccgccuuca cauucacaaa aauccccgcc     1560

gaaacacugc acggaacagu cacaguagaa guacaauacg caggcacuga cggaccaugu     1620

aaaguaccgg cgcaaauggc agucgacaug caaacccuca cgccagucgg acgacuaauc     1680

accgccaauc cagucaucac agaaagcaca gaaaacucaa aaaugaugcu agaacuagau     1740

cccccauucg gagacagcua caucgugaua ggcgucggcg aaaagaaaau cacccaccac     1800

uggcaccgaa gcggcucaac cauaggcaaa gcguucgaag ccaccgucag aggcgcuaaa     1860

cggauggcag uacucggcga cacggcaugg gacuucgguu cagucggggg cgcgcuaaac     1920

agccucggca aaggaaucca ccaaauuuuc ggggccgcau ucaaaagccu cuucggcgga     1980

augaguuggu ucucccaaau acuaaucggc acccuccuga uguggcuagg ccuaaacacc     2040

aaaaacgguu cgaucagccu caugugccua gcguugggag gcgugcuaau auuccuaagc     2100

acagccguaa gugccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     2160

ggcccuccuc cccuccuugc accgagauua auagaucuaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaugcauccc cccccccccc     2280

cccccccccc ccccccccaa aggcucuuuu cagagccacc agaauu                    2326


<210>  9468
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-long mRNA Design 3 opt

<400>  9468
gggagaaagc uuaccaugcg aauaauaaac gcaagaaagg aaaagaaacg gcgcggcgca       60

gacacauccg ucggaauagu gggacuccua cucacaaccg caauggccgc ggaaguaaca      120

agaagaggcu cagcguacua cauguaccua gaccgcaacg acgcaggaga agcaauaucc      180

uuccccacaa cccucggcau gaacaaaugc uacauacaga ucauggaccu cggacacaug      240

ugcgacgcaa ccaugagcua cgaaugccca augcuagaug aaggcgucga acccgacgac      300

gucgacugcu ggugcaacac caccuccacg uggguagucu auggaaccug ccaccacaag      360

aaaggcgaag cccgaagauc aaggagagca gucacacuac caagccacag cacacgaaaa      420

cuacaaacac guucacaaac cuggcucgaa uccagagaau acacaaaaca ccucauacga      480

gucgaaaacu ggauauucag aaaccccggc uucgcccucg cggcagccgc gauagcaugg      540

cugcugggaa gcaguaccuc acaaaaaguc auauaccuag uaaugauccu ccucaucgcg      600

cccgccuaca gcauucgcug cauaggugua agcaaccggg acuucguaga agggaugagc      660

ggcgguacau gggucgacau cguacuagaa cacgggggcu gcguuacagu aauggcacaa      720

gauaagccca ccguagacau agaacucguc acaacaaccg uaucaaacau ggcugagguc      780

agaagcuacu gcuacgaagc auccaucucc gacauggcaa gugacagucg cugccccacc      840

caaggagaag cguaccucga caaacaaucc gacacccaau acgucugcaa acgaacacuc      900

guagaccgcg gauggggaaa uggcugugga cuuuucggaa aaggcagccu aguaacaugc      960

gccaaguucg ccugcagcaa gaaaaugaca ggcaaaucaa uccaaccaga aaaucucgag     1020

uaccgcauua uguuaucagu acacggcagc caacacagcg gcaugaucgu caacgauacc     1080

gggcacgaaa ccgacgaaaa cagagccaaa guagaaauca caccaaacag cccaagagca     1140

gaagccacac uugggggcuu uggcagccua ggccucgacu gcgaaccacg aacaggccuc     1200

gacuucucag aucuauacua ccucaccaug aacaacaaac acuggcucgu acacaaagaa     1260

ugguuccacg acaucccccu ccccuggcau gccggggcag acacgggaac accccauugg     1320

aacaauaaag aagcccuagu agaauuuaaa gacgcccacg ccaaaagaca aaccgucguc     1380

guccucggca gccaagaagg ggccguccac accgcucuag ccggggcccu cgaagcagag     1440

auggacggag ccaagggacg gcuaagcucc ggccaccuaa aaugcagacu caagauggac     1500

aaacuaaggc ucaagggggu cucauacagc uuaugcacag ccgcauucac auucaccaaa     1560

aucccggcag agacauuaca cggcacaguc accgucgaag uacaauacgc aggcacagac     1620

ggcccaugca aagucccagc ccaaauggcg guagacaugc aaacacucac ccccgucggc     1680

cgccuuauca cagccaaccc agucaucacc gaaagcacgg aaaacagcaa gaugaugcuc     1740

gaacucgacc cacccuucgg cgacucguac auaguaaucg gagucggaga gaaaaagaua     1800

acccaucacu ggcaccgcag cggcuccacc auaggcaaag ccuucgaagc gaccguacgc     1860

ggcgcaaaac ggauggccgu acucggcgac acugccuggg acuucggauc aguaggcggc     1920

gcccucaacu cacuaggcaa gggaauccac caaaucuuug gcgcagccuu caaaagccua     1980

uucggcggaa ugucgugguu cagccaaauc cucauaggca cguuacucau guggcucggc     2040

cuaaacacca aaaacggcuc caucucccua augugccucg ccuuaggcgg aguccugauc     2100

uuccucagca cagcaguauc ggccgacgua ggaugcuccg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9469
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-long mRNA Design 3 opt

<400>  9469
gggagaaagc uuaccaugag aauaauaaac gcgcgcaaag aaaagaaacg acgcggcgca       60

gacacaagug ucggaaucgu cggccuccug cugacgacag ccauggcugc agaagucaca      120

cgcagaggaa gcgccuacua cauguaccua gaccgaaacg acgcaggcga agccauauca      180

uucccaacaa cgcucggcau gaacaaaugc uacauucaaa uaauggaccu agggcacaca      240

ugcgacgcaa cgaugagcua cgaaugcccc augcucgacg aaggagucga accagacgac      300

guagacugcu ggugcaacac gaccagcacg ugggugguau acggaaccug ccaccacaag      360

aaaggggaag cacgaagauc acgacgcgcc gugacccucc ccagucacag cacacgaaaa      420

cuccaaacac gcucccaaac auggcuagaa ucacgcgagu acacgaaaca ccucauccga      480

gucgaaaacu ggaucuuuag aaacccaggc uuugcacucg cagcggccgc cauugcaugg      540

cuacugggca guagcacauc ccagaaaguu auauaccucg uaaugauacu ucuaauagcg      600

cccgcauacu ccauccgcug cauaggagug uccaauagag acuucguaga aggaaugagc      660

ggaggcaccu ggguagacgu agucuuagaa cacggaggcu gcgucacagu cauggcccaa      720

gacaaaccga ccguagauau agaacucgua accaccacag ucagcaacau ggcagaagug      780

cgaucauacu gcuacgaagc cucaaucucc gacauggcca gcgacagccg gugcccaaca      840

caaggagaag ccuaccucga caaacaauca gacacccaau augucuguaa acgcacacuc      900

gucgacagag gguggggaaa cggcugcggc cucuucggca aagggucacu cgucacgugc      960

gcaaaauucg caugcagcaa gaaaaugacg ggcaaaucca uccaacccga aaaccucgaa     1020

uaccgaauaa ugcucagcgu ccacggguca caacacagcg gaaugauagu caaugacacc     1080

ggacacgaaa cagacgaaaa cagagcaaaa guagaaauca caccaaacuc accgcgagcc     1140

gaagccacac ugggaggauu cgggagucuc ggccucgacu gcgaaccccg gacuggccua     1200

gacuucuccg accuauacua ccuaacgaug aacaacaaac auuggcucgu ccacaaagaa     1260

ugguuccacg acaucccacu cccguggcac gccggcgccg acaccggcac uccccacugg     1320

aacaacaaag aagcccuugu cgaguucaaa gacgcccacg caaaacggca aacaguagua     1380

gucuuagggu cacaggaggg cgccguucac accgcccucg cuggggcgcu ugaggccgaa     1440

auggacggcg ccaaaggccg ccucuccagc ggacaccuga aaugccgacu uaaaauggac     1500

aaacuacgac uaaaaggagu cuccuacagc cucugcacag cagccuucac cuucacgaag     1560

aucccagccg aaacacuaca cggaacaguc acaguagaag uacaauacgc agggaccgac     1620

ggcccaugca aaguccccgc ccaaauggcc guagacaugc agacacuaac gcccguagga     1680

agacucauca cagcaaaccc gguaaucacc gaaagcaccg aaaacuccaa aaugaugcug     1740

gaacucgauc ccccauucgg agacagcuac auagucaucg gaguaggaga aaagaaaaua     1800

acacaccacu ggcaccgcag cggaucaacc auaggaaaag ccuuugaagc caccguaaga     1860

ggcgcaaaaa ggauggcggu ucugggggac acagcauggg acuucggcuc cguaggcggc     1920

gcacuaaacu cccucggcaa aggaauacac caaaucuucg gagcagccuu caaaucucuc     1980

uucggcggaa ugagcugguu cagccaaauc cuaauaggca cgcuacucau guggcuagga     2040

cuaaacgcca agaacggauc caucagccua augugccugg cucucggcgg cguguuaaua     2100

uuccuuagca cagcagucuc cgccgacgua ggcugcagcg uagacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9470
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-long mRNA Design 3 opt

<400>  9470
gggagaaagc uuaccaugcg caucauaaac gcgcgcaaag aacgcaaacg aagaggcgca       60

gauacaagca uaggaauaau cggacuccug cucacaacag cgauggcagc agagaucaca      120

cgaagggggu cagccuacua cauguaccuc gacagguccg acgcaggaaa agcaauauca      180

uucgcaacca cacuaggagu caacaaaugc cacguacaaa ucauggaccu aggacacaug      240

ugcgacgcca caaugucgua cgaaugcccg augcuggacg aaggagugga accggacgac      300

guagacugcu ggugcaacac aacaaguacg uggguggucu acggaacgug ccaccacaag      360

aaaggagaag cccgacgaag cagacgagca guaacacugc ccagccauuc aacaagaaaa      420

uugcaaacca gaucccagac auggcuggaa agcagggaau acaccaaaca ccucauaaag      480

guagaaaacu ggauauuccg aaaucccgga uucgcacucg uagccguagc aauagccugg      540

uuacugggcu caagcacaag ccaaaaagua auauaccuag ugaugauacu ccugaucgcc      600

cccgcguacu cgauccgaug cauaggaguc agcaaccggg acuuuguuga agggauguca      660

ggaggaacau ggguagacgu cgugcucgaa cacggaggcu gugugacagu aauggcgcaa      720

gacaaaccca cggucgacau agagcuagua acaacaacug uaagcaacau ggcagaagug      780

agaagcuacu gcuacgaagc cucgaucuca gacauggcaa gcgauagccg augcccuacg      840

caaggagaag ccuaccugga caaacaguca gacacccagu acgucugcaa acggacacug      900

gucgaccgcg gcuggggcaa cggcugcggc cucuucggga agggaucacu cgugaccugc      960

gcaaaguuca cgugcagcaa gaaaaugacc ggcaaaagca uacaaccaga aaaccuagaa     1020

uaccgaauca ugcucucagu acacggcucg caacacucag gaaugaucgg auacgaaaca     1080

gacgaagacc gagcaaaagu agaagucacu ccaaacagcc cgcgcgcaga agcaacacua     1140

gggggcuucg ggagccucgg gcuagacugu gaaccacgca cgggacugga cuucagcgac     1200

cucuauuacc uaacgaugaa caacaaacac uggcuggucc acaaagaaug guuccacgac     1260

aucccacuuc cguggcacgc cggagccgac accggcacac cccacuggaa caacaaagaa     1320

gcccuagucg aguucaagga cgcccacgcu aaaagacaaa ccgucgucgu ccucggcuca     1380

caagaaggag cgguccacac cgcgcucgcg ggcgcucucg aagcggagau ggacggugcc     1440

aaaggacgcu uauucuccgg gcaccuaaag ugcaggcuaa aaauggacaa auuaagacuc     1500

aaaggaguau ccuacucgcu cuguaccgca gccuuuaccu ucacaaaggu cccagccgaa     1560

acccuacacg ggacagugac aguugaaguc caguacgccg gcacagacgg accaugcaaa     1620

aucccgguac agauggcggu agacaugcaa acauugacac cagugggacg ccucauaacu     1680

gcuaacccag uaauaacaga aucaaccgaa aacaguaaaa ugaugcugga gcuggacccg     1740

ccauucggag acagcuacau cgugauagga guaggagaca agaaaauaac acaccacugg     1800

cacaggagcg gcagcaccau cggaaaagca uuugaagcga cagucagggg cgcgaaaaga     1860

auggcaguac uaggggacac cgcaugggac uuugggagcg ugggaggagu uuuuaacagc     1920

uuaggaaaag gcauccacca gaucuucgga gccgcauuca agagcuuguu cggaggcaug     1980

agcugguuca gccaaauacu aauagggacc cuccuggucu ggcuugggcu gaacaccaaa     2040

aacggaagca ucagccugac augccucgca cuaggaggcg uaaugauauu ccuaagcaca     2100

gccgugagcg cggacguggg gugcagcgug gacuucagca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  9471
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-long mRNA Design 3 opt

<400>  9471
gggagaaagc uuaccaugcg aaucauaaac gcacggaaag aaaagaagag aagaggagca       60

gacaccagcg uagggaucgu gggccugcuc cucacaacgg ccauggccgc cgaagucacc      120

cgcagaggau cagcuuacua cauguaccua gaccgcaaug acgcgggcga agcaaucagc      180

uuccccacga cccuaggcau gaacaaaugc uacauacaaa uaauggacuu aggccacaug      240

ugcgacgcaa ccaugaguua cgaaugcccg augcuggacg aaggggucga accagacgac      300

guagacuguu gguguaacac cacaucaaca uggguggugu acgggaccug ccaccacaag      360

aaaggcgaag ccaggcgauc gagacgagcu guaacccuac caagccacag caccagaaaa      420

cuacaaacga gaucacaaac guggcuggag ucacgcgaau acaccaaaca ccucauccga      480

guugagaacu ggauauuccg aaacccgggu uucgcauugg cagcggccgc caucgccugg      540

uuacucggaa gcucuacgag ccaaaagguc aucuaccuag uaaugauccu ccucauagca      600

ccagcauaca gcauccgcug uauuggcguc agcaacaggg acuucguaga aggcaugagc      660

gggggcacau gggucgacgu aguacuugaa cacggcggcu gcgucaccgu aauggcacaa      720

gacaaaccca cagucgacau cgaacucgua acaacgaccg uaagcaacau ggcggaagug      780

cgaagcuacu gcuacgaagc aucaaucagc gacauggcga gcgacagcag augcccuacc      840

cagggcgaag cauaccucga caaacaaagc gacacacaau acguaugcaa gagaacucua      900

gucgaccgag gguggggcaa cggaugcgga cuauucggaa aaggcagccu cgucacaugu      960

gcaaaauucg ccugcucaaa gaaaaugaca gguaaaagca uacaacccga aaaccuggag     1020

uaccgcauaa ugcuaagcgu acacgggucc caacacagcg gcaugauagu caacgacacc     1080

ggacacgaga cagacgaaaa ccgagccaaa guggaaauaa ccccaaacag cccaagggca     1140

gaggccaccc uaggaggguu cggcagccuc ggccucgacu gcgaaccacg caccggccua     1200

gacuucucag accuauacua ccucacaaug aacaacaagc acuggcucgu acacaaagaa     1260

ugguuccacg acaucccccu uccauggcac gcgggagccg acaccggaac gccccacugg     1320

aacaacaagg aagcgcucgu ggaguucaaa gacgcccacg caaaacgaca aaccguggua     1380

guccuaggaa gccaagaagg ggcaguacac accgcacucg ccggagcacu agaagcggaa     1440

auggacggag cgaaagggag acucagcagc ggacaccuga aaugucgacu caaaauggac     1500

aagcuacggc ugaagggagu gagcuauucc cucugcacag cagccuucac auucacaaaa     1560

aucccggccg aaacacuaca cggaaccguc acuguggagg uacaauacgc cggcacagac     1620

ggccccugca agguaccagc ccaaauggca guagacaugc aaacacucac accagugggc     1680

cgccuaauaa cggcaaaucc aguuauaacc gagagcacgg aaaacuccaa gaugaugcuc     1740

gaacucgauc ccccguucgg agacucauac auaguaauag gcguagggga gaaaaagauc     1800

acccaccacu ggcacagauc cggcucgaca auaggaaaag ccuucgaagc caccguacgc     1860

ggagcaaaaa gaauggcggu acuaggagac acagcauggg acuucggauc cgugggcgga     1920

gcccuaaacu cccucggaaa gggaauacac cagauauucg gagcggcauu caaaucccua     1980

uucggaggaa ugagcugguu cagccagauc cucauaggaa cacuacuaau guggcuaggu     2040

cucaacacca aaaacggaag cauuagccuc augugccucg cacuaggcgg uguacuaauu     2100

uuccuaagca cagccguauc agcggacgua ggaugcagcg uagacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9472
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU720415.1 prME-long mRNA Design 3 opt

<400>  9472
gggagaaagc uuaccaugcg gaucauaaac gccaggaaag aacgaaaacg gagaggagca       60

gacacgagca uuggcaucau cgggcuacua cuaacgaccg cgauggcggc ggagaucaca      120

cgaagaggaa gcgcauacua cauguaccug gacagguccg acgccggaaa agccauuuca      180

uuugccacaa cacuaggggu aaauaagugc cacguacaaa uaauggaucu uggacacaug      240

ugcgacgcaa caauguccua cgaaugccca augcuggaug agggcguaga accagacgac      300

gucgacugcu ggugcaacac gacaucgacg ugggucguau acggaacaug ccaccacaag      360

aagggggaag cacgaagauc gcggagagca guaacacucc cgagucacuc gacacggaag      420

cuacaaacaa ggagccaaac auggcuggag uccagagaau acacaaagca ccuaauaaaa      480

gucgaaaacu ggaucuuccg caaccccggu uuugcccuag ucgcagucgc aauagccugg      540

cuacuaggcu caagcaccag ccagaaagua auauaucuag ugaugauacu ccugauagcg      600

ccagcguaca gcauuaggug cauaggagua agcaaccgcg acuucguaga aggcaugucg      660

ggcgggaccu gggucgacgu cguauuagaa cacggaggcu gcgucaccgu aauggcacag      720

gacaaaccaa caguagacau cgaacucgua accaccacag ucagcaacau ggcggagguc      780

agaagcuacu guuacgaggc aucuauaagu gacauggcca gugacagccg cuguccaaca      840

caaggagaag cauaccuaga caaacaauca gacacacaau acgucugcaa gagaacccua      900

guagaccggg gaugggggaa cgggugcgga cuuuucggca agggcucgcu cguaacaugc      960

gcaaaauuca cgugcucgaa gaaaaugacg ggcaaaagca uccaaccaga aaaccucgaa     1020

uacaggauaa ugcuaucggu gcacggcuca caacacucag gaaugauagu aaacgacacc     1080

ggauaugaaa cagacgaaaa ccgggcaaag guagagguaa caccgaacag cccccgggca     1140

gaggcgacac uaggcggcuu cggaagccua ggcuuagacu gcgaaccaag aacaggccuc     1200

gacuucucag accucuacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagag     1260

ugguuccacg acauaccgcu cccauggcac gcgggagccg acacaggaac accgcacugg     1320

aacaacaaag aggcccuagu agaguucaaa gacgcacacg caaaaagaca aacaguagua     1380

gugcuaggca gccaagaagg cgccgugcac acagcccucg ccggagcgcu ggaagccgaa     1440

auggacggcg cgaaaggcaa acuguucucc ggacaccuaa agugccggcu caaaauggac     1500

aagcugcgac ucaagggcgu aagcuacucc cuguguacag cagccuucac auucacaaaa     1560

gugccggccg agacccuaca cgggaccgug acaguagaag ugcaauacgc cggaacagac     1620

ggccccugca agaucccggu acaaauggca gucgauaugc aaacacuaac acccgucggg     1680

agauugauaa ccgcaaaccc cguaauaaca gaaaguacag agaacaguaa aaugaugcua     1740

gaacuggacc cgcccuucgg cgacagcuac auagucaucg gcgucggaga uaagaaaauc     1800

acacaccacu ggcaccgaag cgggagcaca auagggaaag cguucgaggc aacagucagg     1860

ggagcgaaac ggauggccgu acuaggcgau accgcguggg acuucggaag ugucggcggc     1920

guauucaacu cacuagggaa aggaauccac caaaucuucg gagcagccuu caagagccua     1980

uucggcggca ugaguugguu cucacaaaua cugauaggca cacugcuggu auggcuaggg     2040

cuaaauacca aaaacgggag cauaagccua accugccucg cacucggugg cgugaugauc     2100

uuccugagca cagccguaag cgcggacgua gggugcagcg uggacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9473
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-long mRNA Design 3 opt

<400>  9473
gggagaaagc uuaccaugag aaucaucaac gccaggaaag agcgaaaacg ucguggagca       60

gacacaucga uaggcauagu cggccuacug cucacaaccg caauggccgc agagauaaca      120

cgcagaggcu cagcauacua cauguaccuc gaccgaagug acgccggaaa agcaaucuca      180

uucgcaacaa cacuaggcgu aaacaaaugc cacguacaaa uuauggaccu aggucacaug      240

ugcgacgcca ccaugucaua cgagugcccg augcuagacg aaggcgugga accagacgac      300

guagacugcu ggugcaacac aacgagcacc ugggucguau acgggacgug ccaccacaag      360

aaaggcgaag caagacgaag ccggcgcgcc gugacacucc caagccacag cacccgaaaa      420

cuacagacca gaagccagac uuggcuagaa agccgagaau acaccaaaca ccucaucaaa      480

guagaaaacu ggaucuucag aaacccaggg uucacgcucg uagccguagc cauagccugg      540

cucuuaggcu caagcacguc gcaaaaaguc aucuaccuag ugaugauacu ccugauagcg      600

cccgccuaca gcauacgaug caucggaguc agcaaccgcg acuucgugga gggcauguca      660

ggagggaccu gggucgacgu aguccuggaa cacggaggcu guguaacagu aauggcccag      720

gacaaaccaa cagucgacau cgaacucgua accaccaccg uguccaacau ggcagaaguc      780

agaucuuacu gcuacgaagc cagcaucuca gacauggccu cagacucacg cugucccaca      840

caaggagaag cguacuuaga caaacaauca gacacccaau acguaugcaa acgcacgcuc      900

gucgaucgag guuggggaaa cggcugcgga cuauucggca aaggcucgcu cguaaccugu      960

gcaaaauuca caugcagcaa gaaaaugaca ggcaaaagca uccaaccgga aaaccuagaa     1020

uaccggauca ugcucucggu acacggcucc caacacagcg gaaugauagu caacgacgag     1080

aaccgcgcaa aaguagaagu caccccaaac ucuccccgcg cagaagcaac ccuaggaggg     1140

uucggauccc uaggcuugga cugcgaaccc agaacaggac ucgacuucag cgaccucuac     1200

uaccuaacaa ugaacaacaa gcacugguug guucacaaag aaugguucca cgacaucccu     1260

cucccauggc augcgggggc agacacaggg acgccucacu ggaacaacaa agaagcccua     1320

gucgaguuca aagacgcgca cgcaaaacgc caaacggucg uaguacucgg cagccaagaa     1380

ggcgcggucc acaccgcgcu agccggcgcu cucgaagcgg aaauggacgg agcaaaagga     1440

cgccuauucu ccggccaccu caagugccga cuaaagaugg acaaacuacg auugaaaggg     1500

guauccuauu cccucugcac agcagcauuc accuucacca agguccccgc cgaaacccua     1560

cacggcaccg ucaccgucga agugcaauac gcaggaaccg acggcccgug caaagucccc     1620

gcacaaaugg ccgucgauau gcagacacua acaccgguag gacgacugau cacugcgaac     1680

cccgugauca ccgaaagcac cgaaaacuca aaaaugaugc ucgaacuaga cccuccguuc     1740

ggcgacagcu acaucgucau cggggucggc gacaagaaaa uaacccacca cuggcaccgc     1800

agcggaucaa cuaucggcaa agcguucgag gccaccguaa gaggcgccaa acggauggca     1860

guacuaggcg auacagcaug ggauuucggg uccguaggag gcgucuucaa cagccucgga     1920

aaaggcaucc accaaauauu cggcgccgcg uucaagagcc uauuuggcgg aaugagcugg     1980

uucucacaaa uccugaucgg aacucuccua gucuggcuag gacuaaacac gaaaaaugga     2040

ucaauaagcc uaacaugccu ggcacuagga ggcgucauga uauuccuaag uacagcaguc     2100

agcgccgacg ucggaugcag cguagacuuc agcaaaugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9474
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-long mRNA Design 3 opt

<400>  9474
gggagaaagc uuaccaugcg caucauaaac gcgagaaaag aacgaaaacg ccgcggggcg       60

gacacgagca ucggcauaau cggacuguug cuaacgaccg cgauggccgc ggagaucaca      120

agacgaggca gcgcguacua uauguaccuc gaccgguccg acgccggaaa agccaucucg      180

uucgccacca cccuaggagu caacaaaugc cacguccaaa uaauggaccu cggccacaug      240

ugcgaugcca caaugucaua cgagugcccc augcuagacg aagguguaga accagacgac      300

guagacugcu ggugcaauac cacgagcacg uggguaguuu acggcacgug ucacagaaag      360

aaaggagaag ccaggagauc aagacgcgcg gucacccucc caagccacuc aacacgcaaa      420

uuacagacca gaagccagac cuggcucgag uccagagaau acaccaaaca ccugauaaaa      480

gucgaaaacu ggaucuuucg caaccccgga uucgcacucg uggcaguagc gauagcaugg      540

cuccuaggca gcagcacauc ccaaaaagug auuuaucucg ucaugauccu acugauagca      600

ccggcguaca gcaucaggug cauaggagua aguaacaggg auuuuguaga aggcaugucu      660

ggcggaacgu ggguagacgu cguacucgag cacggaggcu gugucacagu cauggcccaa      720

gacaaaccca cagucgacau cgagcucguc accacgacag ucagcaacau ggcagaagug      780

cgaucauacu gcuacgaagc gucgaucagc gacauggcau ccgacucgcg cugccccacg      840

caaggugaag cauaccuaga caagcaauca gacacacaau augucugcaa acgcacccuc      900

gucgaccgag gauggggcaa cggaugcgga cucuuuggaa aaggaucccu aguaaccugc      960

gccaaauuua ccugcagcaa aaagaugacg ggcaagagca uccaacccga aaaccucgag     1020

uacagaauca ugcuguccgu ccacggcucg caacacucag gaaugauagu caacgacaca     1080

ggcuacgaaa cagaugaaaa cagggcaaaa gucgaaguca cccccaacuc cccacgcgcg     1140

gaagccaccc ugggcggauu cggcucccua ggccuagacu gcgaaccacg cacaggacuc     1200

gacuucagcg accuguacua ccuaacaaug aacaacaaac acuggcuagu ccacaaggag     1260

ugguuccacg acauaccacu ccccuggcac gcaggcgccg acaccggaac cccgcacugg     1320

aacaacaaag aagcacucgu cgaguucaaa gacgcacacg caaagagaca aacgguagua     1380

guccugggau cccaagaagg cgcaguacac acagcacugg ccggcgcccu agaagccgaa     1440

auggacggcg cgaaaggaaa acuguucagc ggccaccuca aaugccgccu aaaaauggac     1500

aaacuacgac ucaaaggagu aucuuacucc cuauguaccg cagccuucac cuucaccaaa     1560

guaccggccg aaacgcucca cggaacugug acagucgagg uacaauacgc cggcaccgac     1620

ggacccugca aaauaccagu acaaauggcc guagacaugc aaacgcuaac gcccguagga     1680

cggcuaauca cagcgaaccc cgugauaaca gaaucaaccg agaacucaaa aaugaugcuc     1740

gaacuagacc cgcccuucgg cgacagcuau auagugauag gcguaggcga caagaaaaua     1800

acccaccacu ggcacagaag cggauccacc aucggcaaag cguucgaagc aaccguaagg     1860

ggcgccaaaa gaauggcagu ccuaggagau accgccuggg acuucggaag cgucggagga     1920

gucuucaaca gccuuggaaa agggauucac caaaucuucg gagccgccuu caaaagccua     1980

uucgggggca uguccugguu cagccaaaua cuaaucggca cccuauuagu cuggcucggc     2040

cucaauacca aaaauggcag cauuagccua accugccuag cacucggagg cgucaugauc     2100

uuccugagca cagccguaag cgccgacgua ggcugcuccg uagacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9475
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-long mRNA Design 3 opt

<400>  9475
gggagaaagc uuaccaugcg cauaaucaac gccagaaaag aacggaaacg acgaggcgcg       60

gacacaucaa uaggaaucau aggacugcug cugacaacag cgauggcggc cgagauaaca      120

agacgaggcu cggccuacua cauguaccuc gaccggagcg acgcagggaa agcgauauca      180

uucgcaacga cacucggagu aaacaagugc cacguacaaa ucauggaccu cggacacaug      240

ugcgacgcca cgaugucaua cgagugcccc augcuggacg agggcgucga accagacgac      300

guagacugcu ggugcaacac cacaagcaca uggguaguau acggaaccug ccaccacaag      360

aaaggcgaag cgcgaagguc aagacgagca gucacccuac caagccacag cacaagaaaa      420

cuccaaaccc gcucacaaac cuggcuagaa agccgagaau auaccaaaca ccucaucaaa      480

guagaaaacu ggauauuccg caacccaggc uucgcacucg uggcaguagc cauagcaugg      540

uuauuaggca guagcacuag ccaaaaggua aucuaccuag ucaugauacu ccuaaucgcg      600

cccgcguacu caaucagaug caucggagua agcaacagag acuucguaga agggauguca      660

gggggaacau ggguagacgu cguacuagaa cacggaggau gcgucacggu aauggcccag      720

gacaagccaa ccgucgacau agagcucgug accacuaccg uaagcaacau ggcggaagua      780

cgaucauauu gcuacgaagc cucaauauca gacauggccu cugacagccg cugcccaaca      840

caaggagaag cguaccuaga caagcaaagc gacacgcaau acgucugcaa acggacacuc      900

guagaccgcg gauggggaaa cggcugcggu cuauucggaa agggcucgcu cguaaccugc      960

gcaaaauuua ccugcucaaa gaaaaugaca ggcaaaucaa uucagccgga aaaccucgag     1020

uacaggauca ugcucuccgu acacggcuca caacauagcg gcaugacagu caacgacaua     1080

ggauacgaaa cugacgaaaa cagggccaaa gucgaaguaa cgcccaacag cccaagagca     1140

gaagccacac ugggaggauu cggcagcuua ggccuggacu gcgaaccgcg aaccggauug     1200

gacuucucag accucuacua ccuaaccaug aacaacaaac acuggcuagu ccacaaagaa     1260

ugguuccacg acauaccacu accguggcac gcaggcgcag acaccgggac cccgcacugg     1320

aacaacaaag aagcgcuggu cgaguucaaa gacgcgcaug ccaaacgaca aaccgucgug     1380

gucuuaggau cccaagaagg cgcgguccac acggcacugg caggcgcucu cgaagccgaa     1440

auggacggag caaaaggaaa gcuauucagc ggccaccuca aaugcagacu caaaauggau     1500

aaacugaggc ucaaaggggu aucguacucc cugugcacag cagccuucac cuucacaaaa     1560

guccccgcag agacccucca cggcacagug acaguggaag uccaauacgc cgggacagac     1620

ggaccaugca aaaucccagu ccagauggca guagauaugc aaacacugac acccguagga     1680

agacuaauca cggcaaaccc cgugauaaca gaaucgacag aaaacuccaa aaugaugcug     1740

gaauuagacc cacccuucgg cgauagcuac aucguaauag gagucgggga caagaaaaua     1800

acacaccacu ggcaccgcag cggaagcacc aucggcaaag cauucgaggc gacggucaga     1860

ggcgcaaaac gaauggcggu cuuaggagac acagcauggg acuucggguc cgugggaggg     1920

guguucaacu cacuaggaaa aggaauccau caaaucuucg gagccgcauu caaaagccua     1980

uucggaggga uguccugguu cucacaaaua cuaauaggca cacugcuggu auggcuagga     2040

cucaacacca aaaacgggag cauaagccua accugccucg cacuaggggg aguuaugauc     2100

uucuugagca cggccgucag cgcagacguc ggcugcucag uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9476
<211>  2353
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-long mRNA Design 3 opt

<400>  9476
gggagaaagc uuaccaugcg cauaaucaac gcccgcaaag aacgcaaacg aagaggcgca       60

gacacaagca uaggcaucau cggccuccuc cucacaaccg ccauggcagc agaaauaacc      120

cgcagaggaa gcgccuacua cauguaccua gaccgcuccg acgcaggcaa agccauauca      180

uucgcaacaa cccugggagu aaacaaaugc cacguccaaa uaauggaccu gggccacaug      240

ugcgacgcca cgauguccua cgaaugccca auguuagacg aaggaguaga acccgacgac      300

gucgacugcu gguguaacac aacuagcaca uggguagucu auggaaccug ccaccacaag      360

aaaggagaag caagacgauc ccgccgcgcg gugacccugc caagccacuc aacacgaaaa      420

cuacaaacca gaucccaaac cuggcucgaa ucacgugaau acaccaaaca ccucauaaag      480

gucgagaauu ggauauuccg aaacccaggc uucgcccuag uagcaguagc aauagcaugg      540

cugcucggga guagcaccag ccaaaagguc aucuaccuag ucaugauacu acucauagcc      600

ccagcauaca gcauccgaug cauaggagua uccaaccgcg acuucguaga agggaugagc      660

ggaggcaccu ggguagacgu aguccucgaa cauggaggau gcguaacggu aauggcacag      720

gacaagccca cggucgacau agaauuagug acgaccacag uauccaacau ggccgaggua      780

cgaaguuacu gcuacgaagc gucaauaucc gacauggcga gugacucgcg cugccccaca      840

caaggggagg cuuaccucga caaacagagc gacacacaau acgucugcaa acgaacccua      900

gucgaccgcg gaugggguaa cggcugcgga cuauuuggca aaggcagccu agugaccugc      960

gccaaauuca cauguuccaa aaagaugacu ggaaaaucca uacaaccaga aaaccucgaa     1020

uaccgcauca ugcuauccgu acacggcuca caacauucag gcaugauagg guacgaaaca     1080

gacgaaaacc gcgccaaagu agaagucaca ccaaacagcc caagagcaga ggccacacua     1140

ggaggcuucg gaucccucgg acucgacugc gagccgcgaa ccggccucga cuucucggac     1200

cuauacuacc ucacaaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac     1260

aucccccugc cauggcacgc aggcgcagac accggaacac cgcacuggaa caacaaagaa     1320

gcacugguag aguucaaaga cgcccaugcc aaacgccaaa caguaguagu ucugggaucc     1380

caagaaggcg caguccacac ugcccucgcg ggcgcccucg aagcagaaau ggacggcgcu     1440

aaaggcaaac uauucuccgg gcaccucaaa ugucgacuaa aaauggacaa acuccgacua     1500

aaaggcguaa gcuacucucu cugcaccgcc gcguucacgu ucacaaaagu ccccgccgaa     1560

acacuccacg gcacagucac cgucgaaguc caauacgccg gaaccgacgg acccugcaaa     1620

auacccgugc agauggcagu agacaugcaa accuuaacac cagucggacg ccucauaacc     1680

gcaaacccag ucaucacaga aagcaccgaa aacuccaaaa ugaugcuaga acucgacccg     1740

cccuucggcg acuccuacau cgugauaggc gugggggaca agaaaaucac acaccacugg     1800

caccgaagcg gcagcacaau cggaaaagcc uucgaggcca cagucagagg cgcaaaaaga     1860

auggccgugc uaggagacac cgcaugggac uucgguuccg uaggcggcgu auucaacagc     1920

cucggcaagg gaauccacca aaucuucggc gcggcuuuca aaagccucuu cggcggcaug     1980

uccugguucu cacagauccu gaucggcaca cuccucgucu ggcuaggccu aaacaccaaa     2040

aacggcucga uaaguuuaac augccucgca cugggcggag ucaugaucuu ccucucaacc     2100

gcaguuagcg ccgacguagg augcagcgua gauuucucca aaugaggacu aguuauaaga     2160

cugacuagcc cgaugggccu cccaacgggc ccuccucccc uccuugcacc gagauuaaua     2220

gaucuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2340

agccaccaga auu                                                        2353


<210>  9477
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-long mRNA Design 3 opt

<400>  9477
gggagaaagc uuaccaugag aaucaucaac gcacgaaagg aaaagaaacg cagaggagca       60

gacacaagcg ucggcauagu aggacuccua cuaacaacag ccauggccgc agaagucaca      120

agaaggggaa gcgcauauua cauguaccuc gaccgcaacg acgcuggcga agccaucagc      180

uucccgacca cccuaggcau gaacaaaugc uauauacaaa uaauggaccu cggccauaug      240

ugcgacgcaa caaugagcua cgaaugcccc augcucgacg aaggcgucga accagacgac      300

gucgacugcu ggugcaacac aaccucaaca ugggucgucu acggcacgug ccaccacaaa      360

aagggagaag ccagacgcuc ccgacgagcc guuacccucc cuagccacag cacgagaaaa      420

cuccaaacac gaucacaaac cuggcuugaa agccgcgaau acaccaaaca ccucauccgc      480

guggaaaacu ggauauuccg aaaucccggc uucgcccucg cagcagccgc gaucgcaugg      540

cuccuuggcu ccuccacguc ccagaaaguc aucuaucucg ucaugauccu acuuaucgcg      600

ccugcauacu ccauaagaug caucggcgua agcaacagag acuucgucga aggcaugucc      660

ggcggcaccu gggucgacgu cguccuagaa cacggggguu gcguaaccgu cauggcucag      720

gacaaaccaa cagucgacau cgaacuggua acaacgaccg ucagcaacau ggccgaagua      780

cgcagcuacu gcuaugaggc uucaauaagc gacauggcca gcgacagccg gugcccaaca      840

caaggagaag ccuaccuaga caaacaauca gacacccaau acgucuguaa gagaacccug      900

guggaccgcg gauggggaaa cggcugcgga uuguucggca agggcucccu aguaacaugc      960

gcaaaauucg ccugcuccaa gaaaaugacc ggcaaaucga uccaacccga aaaccucgaa     1020

uaccgcauaa ugcuauccgu ccacggcuca caacacucag gcaugauagu aaacgacacc     1080

ggacacgaaa cagacgaaaa uagagcgaaa guggagauca cucccaacag cccaagagcg     1140

gaagccacac uaggaggcuu cggcucccua ggacucgacu gugaaccacg aaccggacuc     1200

gauuucucgg accucuacua ccuaacaaug aacaacaaac acuggcucgu gcacaaagaa     1260

ugguuccacg acauaccccu cccuuggcac gcaggagcag acaccggcac cccacacugg     1320

aacaacaaag aagcccucgu agaguucaaa gacgcccacg ccaaaagaca aacaguugua     1380

guacugggca gccaagaagg agccgugcac accgcccuag ccggagcccu agaagccgaa     1440

auggacgggg cuaaaggccg uuuauccagc ggccaccuaa aaugcagacu aaaaauggac     1500

aaacuaaggu uaaaaggcgu aagcuacucc cuaugcacgg cggcauucac cuucacaaaa     1560

auccccgcag aaacccucca cggaacaguc acaguggaag uucaauacgc cggcacagac     1620

ggacccugua agguaccagc acaaauggcu gucgacaugc agacucugac accaguuggg     1680

cgacucauca ccgcaaaccc cgucaucacu gaaagcaccg aaaacuccaa gaugaugcua     1740

gaacuugacc caccauucgg agacagcuac auaguaauug gcgucggcga aaagaaaauc     1800

acacaccacu ggcacagaag cggcagcacg auagguaaag cauucgaagc aaccguaagg     1860

ggcgccaaac gaauggcagu gcucggagac accgccuggg acuucggaag cguaggaggc     1920

gcccugaacu cacuaggccg aggcauacau caaauauucg gggcagcauu caaaagccuc     1980

uucggcggca ugagcugguu cagucaaaua cuaauaggga cacugcuaau guggcuagga     2040

cugaacacca aaaacggcuc aauaucacua augugccuag cacucggagg cgugcuaaua     2100

uuccuaagca cagcagucuc ggcagacgua ggaugcagcg uagacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9478
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-long mRNA Design 3 opt

<400>  9478
gggagaaagc uuaccaugcg caucaucaac gcaaggaagg agaaaaagcg acgaggcgca       60

gacgcgagcg ucggcauagu aggccuccug cucacaaccg cgauggccgc cgaggucaca      120

cgcagaggcu cggccuauua cauguaccuc gacagaaaug acgcaggcga agccaucuca      180

uucccgacca cacucggaau gaacaaaugu uacauacaaa ucauggaccu agggcacaug      240

ugcgacgcaa cuauguccua cgaaugcccg augcucgacg aaggaguaga accagacgac      300

guagauugcu ggugcaacac aacaagcaca uggguagucu auggcacgug ccaccacaaa      360

aagggggagg cgaggaggag cagaagggca guuacacucc caagccacag cacgcgcaaa      420

cuacaaacaa ggagccagac cuggcuugaa agcagagaau acacaaagca ccugaucagg      480

gucgaaaacu ggauauuccg caacccgggu uucgcacuag cagcagcagc aaucgcaugg      540

cuccuaggaa gcagcacguc ccaaaaagua auauaccucg uaaugauacu gcucaucgcc      600

ccagcuuauu caaucaggug caucggcgug agcaacagag acuuugugga aggaauguca      660

ggcggcacgu gggucgacgu cguucuagaa cacggaggau gcgucacagu aauggcucag      720

gacaaaccaa cagucgacau agagcuagua acaacaaccg uaagcaacau ggcagaaguu      780

cgcaguuacu gcuaugaagc cucgaucuca gacauggccu cagacucgag augccccaca      840

caaggagaag cguaccuaga caaacaauca gacacccaau acguaugcaa aaggacccua      900

guagaccgcg gaugggggaa cgggugcgga cuauucggaa agggaucgcu cguaaccugc      960

gcaaaauucg ccugcagcaa aaagaugacu ggcaaaagca uacaacccga aaaccuggaa     1020

uacagaauca ugcuaucagu ccacgggagc caacacucag gcaugauagu aaacgacacg     1080

ggccacgaaa cagacgaaaa cagagcuaaa guagagauaa cacccaacag cccacgagcg     1140

gaagcgacac uaggagguuu cggaagccua ggccuggacu gcgaaccgcg aacaggccuc     1200

gauuucagcg aucucuacua ccugaccaug aauaacaaac acuggcucgu acauaaagag     1260

ugguuccacg acauaccgcu accauggcau gccggagcag acaccggaac accccacugg     1320

aacaacaaag aggcccuagu cgaguucaaa gacgcgcacg ccaaaagaca aacaguggug     1380

guacugggau cgcaggaggg cgcgguccac acugcacucg ccggcgcgcu agaagccgag     1440

auggacggag caaaaggacg ccuaagcagc ggacaccuca agugccgacu aaagauggac     1500

aagcuccgcc uaaaaggggu cucguacagu cucugcacgg cagcauucac cuucacgaag     1560

aucccggcgg aaacacugca cggaaccgua acaguagaag ugcaauacgc cgggaccgac     1620

ggcccgugca aagucccggc ccaaauggca guagacaugc aaacgcuaac accaguagga     1680

agacugauca cugccaaccc aguaaucaca gaaucgacag agaacucaaa aaugaugcua     1740

gaauuggacc cgcccuuugg cgacagcuac aucgugauag gaguagggga aaagaaaaua     1800

acacaccacu ggcaccgaag cggaagcaca aucgggaaag ccuucgaggc aacaguccga     1860

ggcgcaaaga gaauggcagu ccuuggagac acggcauggg acuucggaag cgucgggggc     1920

gccuugaacu cacuaggcaa agggauccac caaauauucg gagccgcguu caagagccuc     1980

uucggcggca uguccugguu uucacaaaua cucaucggaa cauuacugau guggcucggg     2040

cuuaacacca agaacggcuc aauaagccug augugccucg cccuaggggg cguacucaua     2100

uuccugucca cggccgucag cgccgacgua ggaugcagcg uagauuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9479
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-long mRNA Design 3 opt

<400>  9479
gggagaaagc uuaccaugcg aaucaucaau gcacggaaag agaaaaagcg ccgaggggcc       60

gacacgagcg ucgggaucgu aggccuacug cugaccaccg caauggccgc agaagucaca      120

cgacgaggca gcgcauacua cauguaccuc gacagaaacg acgccggaga agccaucuca      180

uuccccacaa cacucgggau gaacaaaugc uacauccaaa uaauggaccu aggacacaug      240

ugcgaugcaa ccaugagcua cgaaugccca augcucgaug aaggcguuga accagacgac      300

guggacugcu ggugcaacac aacuagcacc ugggucgucu acggcacaug ccaccacaag      360

aaaggggagg cacgcagaag ucgacgggcc guaaccuuac ccagccacuc cacaagaaaa      420

cuacaaacac gcucacaaac cuggcuggaa agucgagaau acaccaaaca ccucauccga      480

guggaaaacu ggaucuucag aaacccggga cuagcacuag ccgccgcagc aaucgcaugg      540

cuacuaggaa gcuccacaag ccaaaaaguc auauaccucg ucaugauccu acugaucgcc      600

ccagcauaca guauacgcug cauaggagug agcaacagag acuucguaga aggcauguca      660

ggaggaacgu gggucgacgu aguccucgag cacggcggcu gcguaaccgu aauggcacaa      720

gacaaaccca ccguagacau agaacucguc accacaaccg uuagcaacau ggcagagguc      780

aggucuuacu gcuacgaagc aagcauaagc gacauggcau ccgacucaag augccccacc      840

caaggcgaag cauaccuaga caaacaaucc gacacacaau acguaugcaa acgaacccua      900

guagaccggg gcuggggcaa cggcugcgga cuauucggca aagggagccu agucacaugc      960

gcaaaauucg cauguuccaa gaaaaugacc ggcaaaucca uccaaccaga gaaccucgag     1020

uaccgcauaa ugcugucagu acacggcucc caacacuccg gaaugauagu gaacgacacc     1080

ggccacgaaa ccgacgagaa cagagccaaa gucgaaauaa cacccaacuc accccgggca     1140

gaggcaaccc ucggaggcuu cggcagucuc ggacucgacu gcgaaccccg uaccggucuc     1200

gacuuuucag accuauacua ccuuacuaug aacaacaaac acuggcuagu gcacaaagag     1260

ugguuccacg auaucccccu accauggcac gccggggccg acaccggcac accacacugg     1320

aacaacaaag aagcgcucgu cgaguucaaa gacgcgcacg caaagcgcca aaccguggua     1380

guccuaggca gccaagaagg ggcaguccac acagcacuag ccggagcgcu ggaagcagaa     1440

auggacggag ccaaaggccg ccuauccagc ggacaucuaa aaugcagacu aaaaauggac     1500

aaacuccguc ucaaaggagu auccuacagc cucugcacag cagcuuucac cuucacgaag     1560

auccccgccg aaacccucca cggcacaguc acagucgaag uccaguaugc cggaacagac     1620

ggaccaugca aagugccggc ccaaauggcc guggacaugc aaacacuuac cccaguaggc     1680

cggcuaauca cagcaaaccc agugaucacc gaaagcaccg aaaacuccaa aaugauguua     1740

gaacuggacc cacccuucgg cgauuccuac aucgucaucg gcgugggcga aaagaaaauc     1800

acacaccauu ggcaccgaag cggcuccacc aucggaaagg cauucgaagc caccguaaga     1860

ggcgccaagc gcauggcagu acuaggagac acagcguggg acuucggcag cgucggagga     1920

gcucuaaaca gccugggaaa ggguauacac caaaucuucg gagcggccuu caaaagccuc     1980

uucgggggca ugucaugguu cagccagauc cuaaucggaa cccuacuaau guggcucggc     2040

cuaaacacca agaacggaag cauaucgcug augugcuuag cacucggcgg aguacuuauc     2100

uuccucucaa cagccguauc cgccgacguc ggcugcagcg ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9480
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-long mRNA Design 3 opt

<400>  9480
gggagaaagc uuaccaugcg gaucaucaac gcacgcaaag agaagaaacg acggggagcc       60

gacaccucag uaggcaucgu gggacuacuu cucacaacgg caauggcagc cgaaguaaca      120

cgcagaggaa gcgccuacua cauguaccua gaccgcaacg acgccggcga agcaaucagc      180

uuccccacaa cccuaggaau gaacaaaugc uacauacaaa uaauggaccu cggccacaug      240

ugcgacgcca caaugucaua cgaaugcccc augcuagacg aaggaguaga acccgacgac      300

gucgacugcu ggugcaacac gacaagcacc uggguuguau acggaaccug ccaccacaag      360

aaaggcgaag cacgccgcuc acggagagcc guaacccucc ccucacacag cacaagaaaa      420

cugcaaacac ggucgcaaac cuggcuagaa ucacgcgaau acaccaaaca cuuaauccga      480

gucgaaaacu ggauauuccg caauccaggc uucgcacucg ccgcagccgc caucgcaugg      540

cuacugggaa gcuccacaag ccaaaaaguu aucuaccucg uaaugauccu acuaaucgcc      600

cccgcuuacu cgauacggug cauaggagua ucgaacaggg acuucguaga gggcaugucc      660

ggcggcacau gggucgacgu cgucuuagag cacgggggcu gcguaaccgu cauggcucaa      720

gacaaaccga ccguggacau cgaacucgua accacaaccg ugagcaacau ggccgaagua      780

agaagcuauu guuacgaagc aagcaucuca gacauggcaa gcgacagcag augcccaacc      840

cagggagaag cauaccuaga caaacaauca gauacacaau acgugugcaa aaggacacuc      900

guagacagag gaugggggaa cggaugcggg cuuuucggaa aaggaucacu cgucacaugc      960

gcaaaauucg caugcagcaa gaaaaugaca ggcaaaucaa uccaacccga gaaccuggaa     1020

uacagaauaa ugcuauccgu ccacgggagc caacacuccg gaaugauagu aaacgacaca     1080

ggacacgaaa cugacgagaa ccgagccaaa gucgagauca caccaaacag cccacgcgcg     1140

gaagccacac ucggcggauu cggaucccuc ggccucgacu gcgagccacg aaccggccua     1200

gacuucucgg accuauacua ccuaaccaug aacaacaaac acuggcuggu gcacaaagaa     1260

ugguuccaug acauaccgcu uccauggcac gcaggagcag acaccggcac accccacugg     1320

aacaacaaag aagcgcuugu ugaguucaaa gacgcccacg ccaaaagaca aaccguagua     1380

guacucggca gccaagaagg ugccguacac acagcgcuug ccggcgcccu cgaagcagaa     1440

auggacggcg caaaaggacg ccuuagcagc ggccaccuaa aauguaggcu caaaauggac     1500

aaacuaagac uaaaaggggu cuccuauucc cuaugcacgg cagcauucac cuucacgaaa     1560

auccccgcag aaacccucca cggaaccgua acagucgaag uacaguacgc aggaaccgac     1620

ggaccgugca aagucccagc ccagauggca guggacaugc aaacccugac gccagucggc     1680

cgacucauca ccgccaaccc ugugaucacg gagagcacag aaaacagcaa aaugaugcuc     1740

gaacucgacc cacccuucgg cgacucguac auagucauag gcguaggcga aaagaaaaua     1800

acacaccacu gguaccgcuc cggcagcaca aucgggaaag cauucgaagc cacagucaga     1860

ggggcgaaac gcauggccgu acuaggagac acagccuggg acuucggcag cguaggaggc     1920

gcacuaaaca gccuagggaa aggcauacac caaaucuucg gagccgccuu caaaagccua     1980

uucgguggaa uguccugguu cucccaaaua cuaauaggca cacuccuaau guggcucgga     2040

cuaaacacca aaaacggauc aaucucccua augugucucg cccuaggcgg aguacucaua     2100

uuucuaucca cagcagucag cgcagacgua ggguguucag ucgacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9481
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-long mRNA Design 3 opt

<400>  9481
gggagaaagc uuaccaugcg aaucauaaac gcccggaaag aaaagaaacg ccgcggagcc       60

gacaccuccg ucggaauagu aggccugcug cucaccacgg ccauggcugc cgaagugacc      120

cgccgaggaa gugccuacua cauguaccuc gacagaaacg acgcaggcga agcaaucagc      180

uuccccacaa cguuaggcau gaacaaaugc uauauacaga uaauggaccu gggccauaug      240

ugugaugcaa ccaugucaua cgaaugcccc augcucgacg aaggagucga accagacgac      300

guagauugcu ggugcaacac aaccucgaca uggguagucu auggaacaug ccaccacaag      360

aaaggcgaag cccgccgcuc aagacgagcg guaacgcuac cuucgcauuc cacacgaaaa      420

cuacaaaccc gcucccaaac auggcuagaa agccgagaau acacaaaaca ccucaucagg      480

guagagaacu ggaucuuccg caacccaggc uucgcacucg ccgcagcagc caucgccugg      540

cuacuaggaa gcagcacauc gcaaaaaguu aucuaccucg uaaugauccu ccuuaucgcc      600

ccggccuaca gcauacgcug caucgggguc uccaacagag acuucgucga aggcaugagu      660

gggggcacgu ggguagacgu cguacuagaa cacggcggau gcgugaccgu cauggcacaa      720

gacaaaccca ccguggacau agagcuagua acaaccacug uaucgaacau ggccgagauc      780

agaagcuacu gcuacgaagc cuccauaucc gacauggcca gcgacagcag augcccgaca      840

caaggagaag ccuaccucga caaacaaagc gacacacaau augucugcaa acgcacccua      900

gucgacagag gcuggggcaa cgggugcggc cucuucggca aaggaagccu cgucacaugc      960

gcgaaauucg caugcuccaa aaagaugacc ggaaaaucga uacaacccga gaaccucgaa     1020

uaccgaauca ugcuaagugu ccacggcagc caacacucag gaaugaucgu caaugacacc     1080

ggacacgaga cagacgaaaa ccgagccaaa guagaaauaa ccccaaacuc accacgggca     1140

gaggcgacgc uaggcggauu cggcucccug ggccucgacu gcgaaccacg gaccggacuc     1200

gacuucagcg accuauacua ccucacgaug aacaacaaac acuggcuagu acacaaagaa     1260

ugguuccacg acaucccacu gccauggcac gcaggcgcag auacaggcac accccacugg     1320

aacaacaaag aggcccuagu agaguucaaa gacgcacacg ccaaaagaca aaccguagug     1380

guacucggca gccaagaagg cgcaguacac acagcacucg ccggcgcacu ggaagcagaa     1440

auggacggag ccaaaggccg gcuauccagc ggccaccuga aaugccgccu caaaauggac     1500

aagcucaggc ucaagggagu aagcuacagc cuaugcaccg cagcauuuac cuucacaaaa     1560

auccccgcgg aaacucugca cggcaccguc acagucgagg uucaauacgc aggcaccgac     1620

ggcccaugca agguccccgc acaaauggcu guggacaugc aaacacucac cccugucggc     1680

agacucauaa ccgccaaccc agucauaacc gagagcacag aaaauuccaa aaugaugcua     1740

gaacuagacc cucccuucgg ggacagcuac aucgucaucg gcguagggga gaaaaagauc     1800

acccaccacu ggcaccgguc aggcucaacc auaggcaaag ccuucgaagc aacaguaaga     1860

ggagccaaac gcauggccgu acugggagau acagccuggg auuucggauc ugucggaggc     1920

gcacuuaaca gccuuggcaa aggcauccac caaaucuucg gcgccgccuu caagucccuc     1980

uucggcggaa ugagcugguu cagccaaauc cuaaucggca cccuccuaau guggcucgga     2040

cucaacacca aaaacgguuc caucucauua augugccuag cccucggagg uguccucaua     2100

uuccuaucca ccgcagucuc cgccgacgua ggcuguagcg ucgacuucuc uaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9482
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-long mRNA Design 3 opt

<400>  9482
gggagaaagc uuaccaugcg caucaucaac gcaagaaaag aaaagaaacg caggggagca       60

gacaccucgg ucggcauagu cggacuccua cucaccaccg ccauggcugc ugaaguuaca      120

cgcagaggau cggccuacua cauguaccug gaccgcaacg acgcagggga ggccauaucg      180

uuccccacca cacuuggaau gaacaaaugc uacauacaaa ucauggaccu cgggcacaug      240

ugcgacgcaa ccauguccua cgaaugcccc augcucgacg aaggaguaga accagacgau      300

gucgacugcu ggugcaacac cacgagcaca ugggucguuu acggcacaug ccaccacaag      360

aaaggugaag cccgccgcag ucgacgagcc gucacccuuc caagccacuc cacacgaaaa      420

cuccaaacca ggagccaaac cuggcucgaa agccgagaau acaccaaaca ccucauaaga      480

guagagaacu ggauauuccg caacccaggc uucgcccucg cagccgccgc caucgcgugg      540

cuccuaggca guagcaccag ccaaaaagug auauaccuag ucaugauacu acugaucgca      600

ccagcauacu caauccggug cauaggagua ucuaaccgag acuucguaga aggcauguca      660

ggagggacau ggguggacgu aguccucgaa cacggagggu gcguaacagu aauggcccaa      720

gacaaaccca ccgucgacau cgagcuaguc accacgaccg ucagcaacau ggcagaaguc      780

cgguccuacu gcuacgaagc aagcaucucg gacauggcca gugacucccg cugccccacg      840

cagggcgaag cauaccucga caaacaaucc gacacccaau acgucuguaa acgcacacuc      900

guagaccgcg gcuggggaaa cggcugcggg cuauucggaa agggcagcuu agucacaugc      960

gccaaguuug caugcucaaa gaaaaugacc ggcaaaagca uacaacccga aaaccucgaa     1020

uaccguauca ugcuguccgu acacggauca caacacagcg gaaugaucgu caacgacacc     1080

ggccacgaaa cagacgaaaa cagagcaaaa gucgaaauaa cucccaauag cccacgcgca     1140

gaggccacac ugggcgguuu cggcagccug ggccuagacu gcgaaccccg cacaggccuc     1200

gacuuuuccg accucuacua ccucaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccaug acaucccccu cccauggcac gccggcgccg acaccggaac cccacacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcccacg cgaaacgaca aaccguagua     1380

guacucggca gccaagaagg cgcaguucau acggcccucg cgggagcacu cgaagcugaa     1440

auggacggcg caaaaggacg acucucauca ggacaucuga aaugccgacu caaaauggac     1500

aaauuaagac ucaaaggcgu auccuacucg cucugcacgg cagcauucac guuuacaaaa     1560

auaccagcag aaacccugca cggcaccguu accguagaag uccaauacgc cgguaccgac     1620

ggcccgugca agguccuagc acaaauggca gucgacaugc aaacauugac acccgucggc     1680

cgacugauca cagcaaaccc cgugaucacc gaaucaaccg aaaacuccaa aaugaugcuc     1740

gaacuggacc caccauucgg cgauagcuau aucgucaucg gagucggcga aaagaaaauc     1800

acccaccacu ggcaccgauc cggcuccaca aucggaaaag cauucgaagc aacaguacgg     1860

ggcgcaaaac ggauggcagu ccucggcgac acagccuggg acuucggcag cgucggcggc     1920

gcccucaaca gccuaggcaa aggcauccac caaauauucg gugcagccuu caagagccuc     1980

uucggaggaa ugagcugguu cagccaaaua cucaucggca cacuccuaau guggcugggc     2040

cucaacacca aaaacggcag cauaagccuc augugucugg cccuaggagg aguacuaaua     2100

uuccuaucca cugccgucag cgcagacguu ggcugcagcg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9483
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-long mRNA Design 3 opt

<400>  9483
gggagaaagc uuaccaugcg caucaucaac gcacgaaagg agaaaaaacg ccgaggggcc       60

gauaccuccg uuggcaucgu gggacuccuc cuaacuacag cuauggccgc ggaagugacc      120

cgccgcggaa gcgcuuacua cauguaccua gaucgcaacg acgccggcga agccaucuca      180

uucccaacga cacugggcau gaacaaaugc uacauccaaa ucauggaccu aggccacaug      240

ugcgacgcca ccaugucuua cgaauguccc acacuugacg aaggcguaga gcccgacgac      300

guagacugcu ggugcaacac gacgucaacc uggguuguau acggcacaug ccaccacaaa      360

aagggagaag ccagacgcag cagaagagcc gucacacuac ccagccacuc gaccagaaaa      420

cuacagaccc gcagccaaac auggcuggaa ucccgcgaau acacaaaaca ccuaauacgc      480

guagaaaacu ggauauucag gaacccgggc uucgcccuag ccgcagcagc aaucgccugg      540

cuccuaggcu ccuccacauc ccaaaaaguc auauaucucg ucaugaucuu acucaucgcc      600

ccagccuacu cuauacgcug caucggcguc uccaaccgcg acuucgucga aggcaugucc      660

ggaggaacau ggguagacgu aguacuggag cacgggggau gcgucacagu aauggcccaa      720

gacaaaccca ccguagacau agaacuaguc accaccacag ucucaaacau ggccgaaguc      780

cgcucauacu guuacgaagc aagcauaucc gacauggccu ccgacucgcg gugcccaacc      840

caaggcgagg ccuaccuaga caaacaaagc gacacccaau acgucugcaa gcguacgcug      900

guggaccgug gauggggaaa cggaugcgga cuauucggca aaggcagccu cguuaccugc      960

gcaaaauucg ccugcuccaa aaaaaugaca ggcaaaucga uucaacccga gaaccucgaa     1020

uaccgaauaa ugcuaagcgu ccacggauca cagcacuccg gcaugaucgu caacgacacc     1080

ggacacgaga cagacgagaa ccgcgcaaaa guggaaauca ccccgaacuc cccccgugca     1140

gaggcaaccc uaggcggcuu cggcucacuc ggacucgacu gugaacccag aaccggccuc     1200

gacuucuccg acuuguacua ccucacaaug aacaacaaac acuggcuagu ccacaaagaa     1260

ugguuccacg acaucccccu ccccuggcac gcaggagcgg acaccggaac cccacacugg     1320

aacaacaaag aagcccucgu cgaguucaaa gaugcccacg caaaacgcca gacagucguc     1380

guccuaggaa gccaagaagg agcaguacac acagcacucg caggagcccu agaagccgaa     1440

auggacggug caaaaggcag acucagcagc ggacaucuca aaugcagacu uaaaauggac     1500

aaacuccggc ucaaaggcgu cagcuacagc cucugcacag cagccuucac auucacaaaa     1560

auccccgcgg aaacccucca cggcacugua acggucgaag uccaauacgc aggaacggau     1620

ggcccaugca aagucccagc ccaaauggca gucgacaugc aaacccucac ccccgucggc     1680

cgucucauaa ccgcaaaccc ggucaucaca gaaagcaccg aaaacagcaa aaugaugcug     1740

gaacuagacc cacccuucgg agauuccuac aucgucaucg gcguaggcga aaaaaaaaua     1800

acccaccacu ggcacagauc agguucgaca aucggaaaag cguucgaagc caccguaaga     1860

ggcgccaaac gcauggccgu ccucggagac accgcauggg acuucggauc uguaggugga     1920

gcccuuaacu cccuaggcaa agguauccac caaaucuuug gagcugcauu caaaucacua     1980

uucggcggaa ugucaugguu cagucaaaua cucaucggga cccuacucau guggcuaggc     2040

cuaaauacca aaaacggguc gauaucacua augugccucg cacucggcgg cguacucaua     2100

uuucucucaa ccgcgguaag cgcagacgug ggaugcucag ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9484
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-long mRNA Design 3 opt

<400>  9484
gggagaaagc uuaccaugcg aauaauaaau gcacgaaagg aaaagaaacg aagaggagca       60

gacacauccg uaggaauagu gggacuacua cuaaccacgg ccauggcagc agaaguaacc      120

aggaggggau cggccuacua cauguaccuc gacagaaacg acgcaggaga agcaaucucc      180

uuccccacaa cacucggaau gaacaaaugc uacauccaga uaauggaucu uggacacaug      240

ugcgacgcca caaugagcua cgagugcccc auguuagacg aaggcguuga accagacgac      300

gucgauugcu ggugcaacac aaccucaacu uggguagucu acgggacaug ccaucacaag      360

aaaggggagg cccgcagguc cagacgagcc guaacacucc ccagccauuc aacacgcaaa      420

cuccagacgc gcagccagac cuggcucgaa agccgagaau acacaaaaca ccuaauccgc      480

guagaaaacu ggauuuuuag aaaccccgga uucgcacucg cagcagcggc uauagccugg      540

cuccucggau ccagcaccag ccaaaaagua aucuaccucg uaaugauccu gcucaucgcc      600

ccagcauaca gcauccgaug caucggagug agcaacagag acuucguaga aggcauguca      660

ggcggcaccu ggguagaugu aguacucgaa cacggaggau gcguaacggu aauggcccaa      720

gacaaaccaa caguggacau cgaacucgug accaccaccg ucuccaacau ggcagaagua      780

cggucauacu gcuacgaagc cucaaucagc gacauggcaa gcgacuccag guguccgacc      840

caaggcgaag cauaccucga caaacaaagc gacacccaau acgucugcaa acggacccua      900

guagaccgag gcugggguaa cggcugcggc cucuuuggaa aaggaagccu cguaacaugc      960

gccaaauuug ccugcagcaa aaagaugaca gggaaaagca uacaaccaga aaaccuagaa     1020

uacagaauaa ugcucuccgu ccacggcagc caacacucag gcaugauagu aaacgacacc     1080

ggacacgaaa cggacgagaa cagagccaaa gucgaaauaa ccccaaacag cccacgcgcc     1140

gaagccacgc ucgggggcuu cggaucauug ggauuagacu gugaaccccg cacaggucuc     1200

gauuucagcg accucuauua ccucaccaug aacaacaagc acuggcuggu acacaaagaa     1260

ugguuccacg acauaccacu ccccuggcac gccggugcag acaccggcac accccacugg     1320

aauaauaaag aagcguuggu cgaguucaaa gaugcccacg cgaagagaca aaccguagua     1380

gugcucggca gccaagaagg agccguacac accgcccucg ccggcgcucu cgaagccgaa     1440

auggacggag ccaaaggucg ccugagcucc ggccaccuaa aaugccgacu aaaaauggac     1500

aaacuuagac uaaaaggagu aucauacuca cucugcacgg cagccuuuac cuucaccaaa     1560

aucccggcag aaaccuuaca cggaacagua acagucgaag ugcaauacgc aggcacagac     1620

ggccccugca aaguccccgc acagauggcc guagacaugc agacccucac cccggucgga     1680

cgccucauca cagcuaaccc ggugaucacc gaauccaccg aaaacagcaa aaugaugcuc     1740

gaacuugacc cacccuucgg cgacagcuac aucgucaucg gagugggaga aaagaaaauc     1800

acgcaucacu ggcaccguag cggaucaacg auaggaaagg ccuucgaggc cacgguaaga     1860

ggcgcgaaaa gaauggcagu ccuaggagac acagccuggg auuucggguc ggucgguggc     1920

gcacucaacu cacuaggcaa aggcauccau caaaucuucg gcgccgccuu caaaagccua     1980

uucggaggaa ugagcugguu uagccaaaua cuuauaggca cacuccuaau guggcucgga     2040

cucaauacaa agaacggaag caucucacua augugucuag cccuaggcgg gguccucaua     2100

uuccuaucca cagucgucag cgccgacguc ggcugcagcg uugacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9485
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-long mRNA Design 3 opt

<400>  9485
gggagaaagc uuaccaugag aaucaucaau gccagaaaag aaaagaaacg gcgcggagcc       60

gacacauccg ucgggauagu aggacuccug cucacaaccg caauggcagc ggaggucacu      120

cgacggggcu cagcguacua cauguaccuc gacagaaacg acgcaggaga agcaauauca      180

uuccccacaa cacuaggaau gaacaaaugc uauauccaga uaauggaccu aggccacaug      240

ugcgacgcga caaugagcua cgagugucca augcucgacg aaggaguaga acccgacgac      300

guagacugcu ggugcaacac caccuccgca uggguagugu acggaacaug ccaccacaag      360

aaaggcgaag ccagacgcuc gcgcagagcc gucacccuac ccagccacuc cacccgaaaa      420

cuucaaaccc gcccacaaac auggcuagaa ucacgggaau acaccaaaca ccuaauacgc      480

guugaaaauu ggauauucag aaaccccgga uucgcacuag cugccgcugc cauagccugg      540

cuacuaggga guucaacauc acaaaaaguc aucuaccuag ucaugaucuu acucaucgca      600

cccgccuaca gcauccgcug uauaggaguc agcaaucgag acuuuguaga gggaaugagc      660

gggggcaccu ggguagacgu cguccuagaa cacggcggau gcguaaccgu cauggcgcaa      720

gacaaaccaa ccgucgacau cgaacucguc acaacaaccg ucucaaacau ggccgaaguu      780

cgaucauacu gcuacgaagc gaguauauca gacauggcca gcgacucccg gugccccacc      840

caaggugaag cauaccugga caaacaaagc gacacccagu acgucugcaa aaggacccug      900

guggaccgcg gauggggcaa cggcugcgga cucuucggca aaggcagccu cguuacgugc      960

gcuaaauucg ccugcagcaa gaaaaugaca ggcaaaucca uccaacccga aaaccuagaa     1020

uaccgaauca ugcuaagcgu acacggcucg caacacucag gcaugauagu caacgacacc     1080

gggcacgaaa ccgacgaaaa ccgagccaaa guagaaauca cgccaaacuc accccgagcc     1140

gaggccacac ucggcggcuu cggaucccuc ggccuagacu gcgaacccag aacaggucuc     1200

gacuucuccg accuauacua ccugacaaug aauaauaaac acuggcucgu ccacaaagag     1260

ugguuccacg auaucccacu gcccuggcau gccggcgcgg acaccggcac accccacugg     1320

aacaacaaag aagcccuagu agaguucaaa gacgcacacg ccaaaaggca aaccgucguc     1380

guccuaggau cccaagaagg cgccguccac accgcccucg ccggcgcccu agaagcagaa     1440

auggacggag ccaaaggccg ccucucauca ggccaccuca agugccgccu aaaaauggac     1500

aagcuacgac uaaaaggcgu gagcuacucc cucugcacag cggccuucac cuucacgaag     1560

auaccagccg aaacacucca cgggaccguc accguagagg uacaauacgc aggaacagac     1620

gggcccugca aaguacccgc ccaaauggcc guagacaugc aaacccucac cccaguagga     1680

cgacucauaa ccgccaaccc agucauaacc gagagcaccg aaaacagcaa aaugaugcua     1740

gaacuagacc cacccuucgg cgauaguuac auuguaauag gcguaggcga aaagaaaauc     1800

acacaccauu ggcacagauc cggaucaacc auuggcaaag cauucgaagc aaccguacgc     1860

ggcgcuaaac gcauggccgu acucggcgau accgcauggg acuuuggauc cgucggggga     1920

gcauuaaacu cacuaggaaa aggcauccac cagauauucg gcgccgcauu caaaucacuc     1980

uucggaggaa ugucaugguu uagccaaaua cucauaggca cacuccucau guggcuaggc     2040

cucaacacca aaaacggaag cauaucccuu augugccucg cccucggcgg uguucucauc     2100

uuucuaucca cagccgucag cgccgacguc ggcugcucag ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9486
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-long mRNA Design 3 opt

<400>  9486
gggagaaagc uuaccaugcg caucaucaac gcccgcaaag aaaagaaacg acgcggcgcu       60

gacaccuccg uaggcauagu aggacuccua cucaccaccg caauggcagc agaagucacc      120

cgccgaggca gcgcauacua cauguaccuc gacagaaacg acgccgguga agccauauca      180

uuccccacca cccucggaau gaacaagugc uacauccaaa uaauggaccu uggccacaug      240

ugcgacgcca ccaugagcua cgaaugccca augcucgacg aaggaguaga gccagacgac      300

gucgacugcu ggugcaacac gaccuccacu ugggucgucu auggcacgug ccaccacaag      360

aaaggagaag ccagaagauc acgccgugca guaacgcuac cgagccacuc aacccgaaaa      420

cuucaaaccc guagccagac cuggcuagag ucacgcgaau acacuaaaca ccugauccgc      480

guagaaaacu ggauauuucg caaccccggc uucgcgcucg cagccgcggc aauagccugg      540

cugcuaggau cguccaccuc caaaaagguu auauaccucg uaaugauccu ccucauagcu      600

cccgcauacu caauaaggug cauaggcguc agcaaccgcg acuucgucga aggcaugucc      660

ggaggcaccu ggguagacgu gguccucgaa cacggaggau gcgucacagu gauggcacaa      720

gacaaaccca ccgucgacau agaacucguc accacuacag ucagcaacau ggccgaaguc      780

agaucauacu gcuacgaagc aagcaucagc gacauggcau ccgacucgcg cugccccacc      840

caaggcgaag cauaccuaga caaacaaagc gacacccagu acguaugcaa acgcacacuc      900

gucgaccggg gcugggggaa cggcugcggu cucuucggaa aaggcucccu cguaaccugc      960

gccaaauucg caugcuccaa gaaaaugacc ggcaaaagca uccaacccga aaaccucgaa     1020

uaccgaauca ugcuaucggu ccacgguucc caacacagcg gaaugauagu caacgacacc     1080

ggacacgaaa ccgacgaaaa ccgagccaaa gucgaaauaa cacccaacag cccgcgagca     1140

gaagccacac uaggcggauu cggaagccuc ggccuagacu gcgagccccg cacaggauua     1200

gacuuuuccg acuuauacua ccuaacgaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccacu accauggcac gcaggggccg acaccggaac accacacugg     1320

aacaauaaag aggcacucgu agaauuuaaa gacgcacacg caaaacgaca gaccguuguc     1380

guacucggau cccaggaggg agccguccac accgcacuag ccggugcccu agaagcagaa     1440

auggacggag caaaaggaag acuaaguagu gggcaccuca aaugccgucu aaaaauggac     1500

aagcucaggc uaaaaggcgu gagcuacucc cucuguacag ccgccuucac cuucaccaaa     1560

auccccgcug aaacacuaca cggaaccguc accgucgaag uccaauacgc cggcaccgac     1620

ggccccugca aaguucccgc ccagauggcc gucgacaugc aaacccucac acccgucggu     1680

cgacucauca cagcgaaccc cgugauaacc gaaagcacug aaaauagcaa aaugaugcuc     1740

gagcuggacc cacccuuugg ggacagcuac auaguaaucg gcgucggcga aaagaaaauc     1800

acccaccacu ggcacagaag cgggagcacc auaggaaaag cauucgaagc aacaguccgc     1860

ggugcaaaac guauggccgu acucggagac acagccuggg acuucggcag cguaggcggu     1920

gcacuaaaca gccuaggaaa aggaauacac caaauauucg gagccgccuu caaaagccuc     1980

uucggaggca ugucgugguu cucgcagauu cucauaggca cacuucuaau guggcuaggc     2040

cuaaacacca agaacggcuc aaucagccua augugccugg cacucggagg cguccuaauc     2100

uuccuaucaa cagcagucag cgcagacguc ggaugcagcg uagacuuuuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9487
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-long mRNA Design 3 opt

<400>  9487
gggagaaagc uuaccaugag aaucaucaac gcacgaaaag aaaagaaacg acgcggagcc       60

gacacauccg uaggcaucgu uggacuacua uuaaccacag caauggccgc cgaaguaaca      120

agaagaggua gcgcauacua cauguaucuu gaccggaacg acgccggcga agccaucagc      180

uucccgacca cacuagggau gaacaaaugc uacauucaaa uaauggaccu gggccauaug      240

ugcgacgcaa caaugucaua cgaaugcccg augcucgacg agggcguaga accggacgac      300

gucgauugcu ggugcaacac aacuucaacu ugggucgucu acgguaccug ccaccacaag      360

aaaggcgaag cccgccgcuc gcggcgcgca guaacgcuac ccagccacuc cacgcgcaaa      420

cuacaaaccc gcucccagac cuggcuagaa ucgcgagagu acacaaaaca ccuaauccgc      480

guggaaaacu ggauuuuccg aaacccaggc uucgcccucg cagccgcagc gaucgcaugg      540

cuccuaggca guagcacacg acaaaaagua auauaucuug uaaugauacu ucucaucgcc      600

cccgccuaca gcauccgaug cauuggcguc agcaacaggg acuucguaga aggcaugucc      660

ggaggaaccu ggguagacgu cguucucgaa cacggcggcu gcgucaccgu aauggcacaa      720

gauaaaccga ccguagacau cgaacuugug accaccacgg uguccaacau ggccgaagua      780

agaagcuacu gcuaugaagc aagcaucagc gacauggcca gcgacagcag augccccaca      840

caaggcgagg cuuaccucga uaaacaauca gacacccagu acguaugcaa gcgaacccua      900

gucgacagag gcuggggaaa cggcugcggc cucuucggua aagggucacu cguuaccugc      960

gccaaauucg ccugcagcaa gaaaaugacc ggcaagagca uccaacccga aaaccucgaa     1020

uaccgaauca ugcugucagu ccacgguagc caacacucag guaugauagu caacgacacc     1080

ggacacgaaa cagacgaaaa cagagcaaaa gucgaaauca cacccaacag cccuagagcg     1140

gaagccaccc ucggaggcuu cggcagccua ggccuagacu gugaaccacg cacaggccuc     1200

gacuucagcg acuuauauua ucucacaaug aacaacaagc acuggcuagu ccacaaagaa     1260

ugguuccacg acauaccacu acccuggcac gcaggagcag acaccggcac accccacugg     1320

aacaacaaag aggcacucgu ugaauuuaaa gacgcacacg ccaaacgaca aaccgucgua     1380

guacuaggaa gccaagaagg cgcaguacac acagcccuag ccggggcgcu agaagccgaa     1440

auggacggcg ccaagggacg ccucuccucc ggacaccuca aaugucggcu aaaaauggac     1500

aaacuaagac uaaaaggggu auccuacagc cucugcacgg cagccuucac cuuuaccaaa     1560

aucccagccg aaacccuaca cgggacaguc acaguagaag uucaauacgc cgggaccgac     1620

ggaccaugca agguccccgc gcaaauggcc guggacaugc aaacccuaac accuguaggc     1680

cgacucauaa ccgccaaccc cguaauaacc gaaagcaccg aaaacuccaa aaugaugcuc     1740

gaauuagauc ccccauucgg cgacagcuac auaguaauag gcguaggcga gaaaaagauc     1800

acgcaccacu ggcacagauc cggcagcacc aucggaaaag ccuucgaggc aaccgugcga     1860

ggcgccaaac gcauggccgu ccucggagac acggcauggg acuucggauc aguaggcgga     1920

gcccuaaaca gccuaggaaa aggaauacac caaauauucg gcgcagccuu caaauccuua     1980

uucggcggca ugagcugguu cucacaaaua cuaaucggga cccugcucau guggcucgga     2040

cucaacacca aaaacggaag cauaucccuc augugccucg cacucggggg cguacuaauc     2100

uuccugagca cagcaguauc cgccgacgua gguugcucgg ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9488
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-long mRNA Design 3 opt

<400>  9488
gggagaaagc uuaccaugcg cauaaucaac gcgcgaaaag aaaagaagcg aagaucggca       60

gacaccagcg ucggcauagu cggccuacuc cucaccaccg cgauggccgc agaagucaca      120

cgcaggggcu ccgcauacua cauguaccuc gaccgaaacg acgccggcga agccaucagu      180

uuccccacaa cacuaggaau gaacaaaugc uacauacaaa ucauggaccu ggggcacaug      240

ugcgacgcca caauguccua cgaaugcccc augcuggacg aaggcgugga accggacgac      300

guagacuguu ggugcaauac caccagcacc uggguagugu auggcacaug ccaccacaag      360

aaaggagaag ccagacgcuc acggcgcgca gugacacucc caagccacag cacucguaaa      420

cuacaaaccc gaagccaaac cuggcuagaa ucccgagaau auacaaaaca ccucauaaga      480

guagaaaacu ggauauuccg aaaccccgga uucgcgcucg cagccgcagc aauagcaugg      540

cuacuuggcu caagcaccag ccaaaaaguc auauaccuag ucaugauacu gcucaucgcc      600

ccagcauacu caaucagaug cauaggcgua agcaaccgag acuucguuga agggaugucu      660

ggagggacau gggucgacgu cguacuagaa cacggaggcu gcgucacggu cauggcccag      720

gacaaaccaa cgguagauau agagcucgua acgacaaccg uaagcaacau ggcagaagua      780

cgcaguuacu gcuacgaagc cagcauauca gauauggccu ccgacucccg cugccccaca      840

cagggagagg ccuaccucga caaacaauca gacacacaau acgucugcaa acggacgcua      900

guagaccgcg gcuggggaaa uggaugcggu cucuucggca aaggaucgcu agugacaugu      960

gccaaauucg caugcuccaa gaaaaugaca ggcaaaucca uccaaccaga aaaccuagag     1020

uacagaauaa ugcucagcgu acacggcagc caacacagcg gcaugauagu caaugacaca     1080

ggacacgaaa ccgacgaaaa ccgggccaag guagagauaa cccccaacag cccaagagca     1140

gaggcgaccc ugggcgguuu cggcagccuc ggccuagacu gcgagccaag aacaggacuc     1200

gacuucagcg accucuacua ccuaacaaug aacaacaaac acuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu accauggcac gccggcgcag acaccgggac accacacugg     1320

aacaacaagg aagcccuagu cgaguucaag gacgcacacg caaaacgaca gaccgucgua     1380

guacuaggau cacaagaagg ggccguacac acagcccuag ccggugcacu agaagcagaa     1440

auggacggag caaaaggacg ccuaucaagc ggccaccuaa aaugccgccu caaaauggau     1500

aaacuucguc uaaaaggagu cagcuacagc uuaugcacug cagcauucac auucacaaaa     1560

aucccagcag aaacacuaca cggaaccguc acaguggaag uccaauacgc cggaacagac     1620

ggcccaugca aaguaccagc ccaaauggcg gucgauaccc agacacucac cccagucggg     1680

cgacucauaa cagcaaaccc agucaucacc gaaucgacag aaaacucaaa aaugaugcua     1740

gaacucgacc cacccuucgg cgacagcuau aucguaauag ggguaggaga gaaaaagaua     1800

acacaccacu ggcaccggag cggcagcacc auaggcaaag cguucgaagc cacaguccgu     1860

ggagcaaaaa gaauggcagu auugggggau acggccuggg acuucggcag cguaggagga     1920

gcccucaaca gccuaggaaa agggauccac caaauauucg gcgccgcguu caaaagccug     1980

uucggaggaa ugagcugguu cucccaaaua cuaaucggga cccuccucau guggcucgga     2040

cuuaacacca aaaacggaag caucucacua augugccuag cacuaggagg agugcuaauu     2100

uuccugagua cagccguauc cgcggacguc ggcugcuccg uugacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9489
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-long mRNA Design 3 opt

<400>  9489
gggagaaagc uuaccaugag aaucaucaac gcaagaaaag aaaagaaaag acgaggagcc       60

gacacaaacg uaggaaucgu cggacuacuc cucacaacug caauggcagc agaagucacg      120

cgacgaggau ccgccuacua cauguaccua gaccgcaacg acgcgggcga agcaauauca      180

uucccaacca cccuggggau gaacaaaugc uacauacaga uaauggaccu cggacacaug      240

ugcgacgcga ccaugagcua cgagugccca augcucgacg aaggcgugga accagacgau      300

gucgacugcu ggugcaacac cacgagcaca ugggucguau acgggacaug ccaccacaag      360

aaaggagaag cgaggcgauc ccggagagcc gucacccucc cgagccacuc aacacgaaaa      420

cuacaaacaa gaucgcaaac cuggcuagaa ucgagagaau auaccaaaca ccuaauaagg      480

guagaaaacu ggaucuuccg caacccgggc uucgcgcuag ccgcagccgc aauagccugg      540

cugcuaggga gcucaacaag ccagaaagua aucuaucuag uaaugauccu ccuaauagcc      600

ccagcauaca gcauccgcug cauaggcgua agcaacaggg auuucguaga aggaaugagc      660

ggaggcacau ggguagacgu agugcucgag cauggaggau gcgucacagu cauggcacaa      720

gacaaaccaa cagucgacau agaacuggua acaaccacag uguccaacau ggcagaaguc      780

cgcagcuacu gcuacgaagc cagcauaagc gacauggcaa gcgacagccg gugcccaaca      840

caaggagaag ccuaccuaga caaacaaucc gacacacaau acguuugcaa acgcacguua      900

guagaccgcg gauggggaaa cgguugcgga cucuucggca aagguagcuu ggugacaugc      960

gccaaauucg ccugcaguaa gaaaaugaca ggcaaaagca uccaaccaga gaaccuagag     1020

uacagaauca ugcucagcgu ccacggcucc caacacagug gaaugaucgu aaacgacaca     1080

ggacacgaga cggacgaaaa cagagcaaaa guagaaauca cacccaacag cccacgggca     1140

gaagccaccu ugggaggauu cggaucccua ggucucgacu gcgaacccag aaccggauua     1200

gauuucuccg accucuacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acaucccauu accauggcac gccggugccg acacaggaac cccgcacugg     1320

aacaacaaag aagcccuagu ggaguucaaa gacgcccacg caaaaagaca aacaguagug     1380

guucuaggau cccaagaagg ugcaguccac acugcacugg cgggugcacu cgaagccgag     1440

auggacggcg caaaaggccg gcuaucgagc ggccaccuaa aaugcaggcu caagauggac     1500

aagcuaagac ucaaaggcgu gagcuacuca cucugcaccg cagcguucac auucaccaaa     1560

aucccagccg aaacccuaca cgggacaguc acaguggagg uccaguaugc aggcaccgau     1620

ggcccaugca aaguaccggc ccaaauggca guagacaugc agacacuaac accggucggc     1680

cgacuaauaa cagcaaaccc aguaaucaca gaaagcaccg agaacucgaa aaugaugcua     1740

gaacucgauc ccccauucgg cgacagcuac aucguaaucg ggguagggga gaagaaaauc     1800

acccaccauu ggcaccggag cggcagcacg aucgggaaag cauucgaagc cacgguacga     1860

ggagcgagac gaauggcagu ccucggggac accgcauggg acuucggcag uguuggcgga     1920

gcauugaacu cgcucgggaa agggauacac cagauuuucg gggcagcauu caaaucacua     1980

uucggaggga ugagcugguu cagccagauc cucaucggaa cccuacugau guggcuugga     2040

cuaaacacca aaaacggauc cauaagccua augugccuag cccugggagg aguccuaaua     2100

uuccuaagua cagccguaag ugccgacgua ggaugcagcg ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9490
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317938.1 prME-long mRNA Design 3 opt

<400>  9490
gggagaaagc uuaccaugcg aaucauaaac gcccgaaaag aaaagaaacg acgcggagca       60

gaaaccucgg uaggcauagu aggacugcua cuaaccacag cgauggcagc cgaaguaaca      120

agaagagguu cagccuacua cauguaccuc gaccgcaacg acgcaggcga agcgaucagc      180

uucccaacga cgcuaggaau gaacaaaugc uacauacaaa uaauggaccu gggccauaug      240

ugcgacgcca cgaugaguua cgaaugcccc auguuggacg agggagucga gccagacgac      300

gucgacugcu gguguaacac aacgagcacg ugggucgucu acggaaccug ccaccacaag      360

aaaggcgaag cccgacgauc aagacgcgca guaacacuac cgagccacuc cacacgcaaa      420

cuccaaacaa gaagccaaac cuggcucgaa agccgagaau acacaaaaca ccuaauccgc      480

guagaaaacu ggaucuuccg caacccaggc uucgcacuag cagccgccgc aauagcaugg      540

cuccucggua gcagcaccag ccaaaaaguc aucuaucuag uaaugauacu ccuuauagcg      600

ccagcauacu ccauucgaug caucggcguu aguaaccgcg acuucguaga aggaaugucg      660

gguggaaccu ggguagacgu gguacucgag cacgguggcu gcguaacggu gauggcgcaa      720

gacaaaccca cagucgacau cgaacucgug acaacaacag uaagcaacau ggccgaaguc      780

cggucauacu gcuacgaggc aucaaucagc gauauggccu cagacagccg cugccccacg      840

cagggcgaag ccuaccucga caaacagucc gacacacaau acgucugcaa acgaacgcua      900

gucgacaggg gaugggggaa cggcugcgga cucuucggca aaggaucccu aguaaccugc      960

gcaaaauucg ccugcucaaa gaaaaugaca ggaaaaagca uucaaccaga aaaucuagaa     1020

uacagaauca ugcugucagu ucacggcuca caacacagcg gcaugauagu aaacgacaca     1080

ggccacgaaa cagacgagaa ccgagccaaa guagaaauca cucccaauag cccaagagca     1140

gaagcaaccc uuggaggguu cgggucacuu ggacuagacu gcgaaccacg aaccggccua     1200

gacuucagcg accuuuacua ccucaccaug aacaauaaac acuggcucgu acauaaagaa     1260

ugguuccacg acaucccacu gccguggcac gcaggcgccg acacagguac accacacugg     1320

aacaacaaag aagcacuggu agaguucaaa gacgcccaug cgaaacgcca gaccgucguu     1380

gugcugggcu cacaagaagg agccguacac acagcacugg ccggagccuu agaagccgaa     1440

auggacggcg cgaaaggacg cuuguccucc ggccaccuca aaugccggcu aaaaauggac     1500

aaacuacgcc ugaaaggagu uucguauuca cugugcacag cagcguucac cuucaccaaa     1560

auaccagccg aaacccuaca cggcacagua accgucgaag uacaauacgc gggaaccgac     1620

gggcccugca aaguuccagc acaaauggcg guagacaugc aaaccuuaac accgguaggc     1680

agacuaauca cagccaaccc agucaucacc gaaaguacgg aaaauucgaa aaugaugcua     1740

gaacuagacc cgccauucgg agacagcuac auaguaaucg gggucggcga aaagaaaaua     1800

acacaccacu ggcacagaag cggcagcacg auaggcaaag ccuucgaagc caccgucaga     1860

ggcgcgaaac ggauggcagu acuaggcgac accgccuggg acuucggcag cgucggcgga     1920

gcccucaaca gcuuaggcaa gggaauccac caaaucuucg gcgccgcauu caagagccuc     1980

uucggcggca ugucgugguu cucccaaauc cucauaggca cucuacuaau guggcuagga     2040

cucaacacga aaaacggcag cauaagccug augugccucg cguuaggcgg aguccucaua     2100

uuccugagca cagccgucuc agcggacguc gggugcagcg uggacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9491
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-long mRNA Design 3 opt

<400>  9491
gggagaaagc uuaccaugag aauaaucaac gcccgcaaag agaaaaagcg cagaggcgca       60

gacacauccg uaggcauagu aggccuacuc cugacaacag caauggccgc cgaaguaacc      120

cgucgaggau cagccuacua cauguaucua gaccgaaacg acgccggaga agcuaucagc      180

uuccccacca cacucggcau gaauaagugu uacauccaaa ucauggaccu cggccacaug      240

ugcgacgcaa ccaugagcua cgaaugccca augcuggacg aaggcguaga acccgacgac      300

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggaaccug ccaccauaag      360

aaaggggaag ccagacggag ccgacgugcg guaacgcucc ccagccacuc aacucgcaaa      420

cuccaaaccc gcagccaaac guggcucgaa agccgggaau acaccaaaca ccuaaucaga      480

guggaaaacu ggauauuccg aaaccccgga uucgcgcucg cagcagccgc gauagcuugg      540

cugcucggaa gcuccaccuc ccagaaagua auauaucuag ucaugauccu ccucauagca      600

cccgccuaca gccuccgcug cauaggcgua uccaaccgag acuucgugga aggcaugagc      660

ggcggcaccu ggguagaugu cguacucgaa cacggaggcu gcgucaccgu aauggcccaa      720

gacaaaccca caguagacau agaacuuguc acaaccaccg uauccaacau ggccgaagug      780

cgguccuacu gcuacgaagc cagcauauca gacauggccu cggacucacg augcccaacc      840

caaggcgaag ccuaccucga caaacaaagc gacacccaau acguaugcaa acgcacacuc      900

guagacagag gauggggcaa cgggugcggu cuuuucggaa aaggaucacu cgucacaugc      960

gcaaaauucg ccugcuccaa aaagaugacc ggaaaaucaa uccaaccaga aaaccuagaa     1020

uaccgcauca ugcucagcgu acauggcucc caacacuccg gcaugauagu caacgacacc     1080

ggacacgaaa ccgacgaaaa ccgcgcaaaa guagaaauca cacccaacag cccacgagcc     1140

gaagccaccc ugggaggguu cgggagccua ggacucgacu gcgaaccgcg aacgggacua     1200

gacuucuccg accucuacua ccuuaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acaucccauu accauggcac gccggcgcug auaccggcac cccacacugg     1320

aacaacaaag aagcccucgu cgaguucaaa gacgcucacg cgaaacgcca aaccguaguc     1380

guacucggcu cacaagaagg cgccgugcau accgcacuag caggggcacu agaagcagaa     1440

auggacggcg caaaaggccg ccucagcucu ggccaccuua aaugcagacu aaaaauggac     1500

aagcuacgcc uaaagggcgu gagcuacuca cugugcaccg cagccuuuac cuucaccaaa     1560

auccccgccg aaacucucca uggaaccgua acagucgaag uccaguacgc cggcacggac     1620

ggaccaugca aaguccccgc acaaauggcc guugauaugc aaacacugac acccguggga     1680

agacucauca ccgccaaccc agucaucacc gaaagcacag aaaacucaaa aaugaugcug     1740

gaauuagacc caccauucgg cgacucauac aucgugaucg gggucggaga aaagaaaaua     1800

acacaccacu ggcaccgcag cggaucaaca auaggcaaag cauucgaagc cacagucaga     1860

ggggcaaaaa gaauggcggu acuaggagac acagcauggg acuucggcuc agugggcgga     1920

gcccucaacu cccuuggaaa aggcauucac caaaucuucg gggcagcauu caaaucccua     1980

uucggaggaa uguccugguu cucgcaaaua cucaucggca cgcuccuaau guggcuagga     2040

cuaaacacca aaaacggaag uauaucccua augugccucg cgcucggagg cguacuaauu     2100

uuccugagca ccgcagucag cgcagaugua ggcugcuccg ucgacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9492
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-long mRNA Design 3 opt

<400>  9492
gggagaaagc uuaccaugcg cauaaucaau gcacgaaagg agaaaaagcg caggggagcc       60

gacaccaacg uaggcauagu cggccuacuu cuaacaacag caauggcggc cgaaguaaca      120

agacgcggau cagccuacua cauguaccuc gaccgaaacg acgccggcga agccaucagc      180

uuccccacca cccucggcau gaacaaaugc uacauacaga uaauggaccu cggucacaug      240

ugcgacgcca caaugucaua cgaaugcccc augcuagacg agggagugga accggacgac      300

gucgacugcu ggugcaacac caccagcacc uggguagucu acggaaccug ccaucacaag      360

aaaggcgaag ccagaagauc ccgacgcgcu guaacccucc ccagccacag cacucgcaaa      420

cuccaaacca ggucacaaac cuggcuagaa ucucgagagu acacaaaaca cuuaauacga      480

guagaaaauu ggauguuccg aaaccccgga uucgcccucg cggcagcagc aauagccugg      540

cuccucggau ccuccacauc gcaaaaaguc auauaccuug uuaugauccu acuaaucgca      600

ccggcauaca gcauccgcug caucggaguc ucaaacagag acuucgucga aggaaugucg      660

ggcggcacau gggucgacgu cguucuugag cacggaggau gcgucacggu cauggcucaa      720

gacaaaccua caguagacau agagcugguu acaacaaccg uguccaacau ggccgagguc      780

cgguccuacu gcuacgaggc auccaucagc gacauggccu ccgacucacg augcccaacc      840

caaggggagg cuuaccucga caaacaaagc gacacacaau acgucugcaa acgaacacug      900

guugauaggg gauggggcaa cggcugcggc cuuuuuggca aaggaagccu ggucacaugc      960

gccaaguucg ccugcagcaa gaaaaugacg ggcaaaagua uacaaccaga aaaccuggaa     1020

uaccgaauca ugcucagcgu ccacgguucc caacacuccg gcaugauagu aaacgacaca     1080

ggccacgaaa cagacgaaaa cagagcaaaa gucgaaauca cccccaacag cccacgggcc     1140

gaagccacac ugggcgguuu cggcagccuc ggacucgacu gcgaaccgcg cacgggacuc     1200

gacuucucag accucuacua cuuaacaaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauuccccu accauggcau gccggagcgg acaccggcac accccacugg     1320

aacaacaaag aagcacuggu cgaguucaaa gacgcacacg ccaaacggca aacagucguc     1380

guccuaggau cacaagaagg agcaguccac acggcacuag cgggcgcccu agaagccgaa     1440

auggauggcg caaaaggccg gcuaucaagc ggacaccuaa aaugcagacu aaaaauggac     1500

aaacuuagac ucaaaggcgu aagcuacagc uuaugcacag ccgccuucac cuucacgaaa     1560

auccccgcag aaacacucca ugguacaguc acgguagaag ugcaauacgc cggcacagac     1620

gggccaugca aagucccugc acaaauggca gucgacaugc agacccuaac cccagucggc     1680

cgacucauaa cggccaaucc agucaucaca gagucaaccg agaacagcaa aaugaugcuc     1740

gagcucgacc cgccauucgg cgacagcuau aucgucaucg gugucggcga aaagaaaauc     1800

acacaccauu ggcacaggag cgguagcaca auaggcaagg cauucgaagc aaccguccgc     1860

ggcgccaaac gcauggccgu ccuaggagac accgccuggg acuucggauc aguagggggc     1920

gcccucaaua gccugggcaa aggcauccau caaaucuucg gggcagccuu caaaagccuu     1980

uucgguggaa uguccugguu cagccaaauc cucauaggca cccuccuaau guggcucggc     2040

cucaacacga aaaacggguc cauaucccuc augugucuag cacuaggcgg aguccucauc     2100

uuccucucca ccgcggucuc ggccgacguc ggaugcucgg uagauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9493
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-long mRNA Design 3 opt

<400>  9493
gggagaaagc uuaccaugag aauaaucaac gcccgaaaag agaaaaagcg ccgaggcgcc       60

gacacaagcg ucggcaucgu cggacuucuc cuaacaacag ccauggccgc ggaaguaaca      120

agaaggggca gugcauacua cauguaccuc gaccgcaacg acgcaggaga agccaucagc      180

uuccccacaa cauuaggcau gaauaaaugc uacauacaga ucauggacuu gggccacaug      240

ugcgacgcca caaugagcua cgaaugcccc augcucgacg aaggcguaga accagacgac      300

gucgacugcu ggugcaacac aacaagcacg ugggugguau acggaaccug ccaccacaag      360

aaaggagaag caagacgaag ccguagagca gugacacucc ccagccacuc gaccagaaaa      420

cugcaaacac gcagccaaac cuggcucgaa ucacgagaau acaccaaaca ccucauccga      480

gucgaaaacu ggauacuacg gaaccccgga uuugcacucg cagcagccgc caucgcaugg      540

cuacucggcu caucgaccuc ccagaaaguc auauaccuug uaaugauacu ccuaaucgcg      600

cccgccuaca gcauccgaug caucggcgug ucuaaucgag acuucguaga aggcaugagc      660

ggaggcacau gggucgacgu cguacuggag cacggaggcu gcgugacggu aauggcacag      720

gacaaaccca cagucgacau agagcuaguc acaacgaccg ucuccaacau ggcagaagua      780

cgcagcuacu guuacgaagc cagcauaagc gacauggcca gugacagccg gugcccaacc      840

caaggggaag ccuaccuaga caaacaaagc gacacacaau acguaugcaa aagaacccuc      900

gucgaucgag gcuggggaaa cggaugcggc cucuucggga aaggaucccu cgugaccugc      960

gccaaauucg caugcagcaa gaaaaugacc ggaaaaagua uacaaccaga aaaccuagag     1020

uaucgcauaa ugcuaucagu ccacggcucc caacacuccg ggaugauagu caacgacacc     1080

ggccacgaaa cagacgaaaa ucgcgcaaaa gucgaaauca ccccaaacag cccaagagcu     1140

gaagccaccc uaggaggauu cggaucccuc ggccucgacu gcgaaccacg aacaggccuc     1200

gacuuuuccg acuuauauua ccucacaaug aacaacaaac acuggcuagu ccacaaggaa     1260

ugguuccacg acaucccacu acccuggcac gcaggcgcag acacaggcac cccacauugg     1320

aacaacaaag aagcacucgu ggaguucaaa gacgcucacg caaaacgcca aacaguagua     1380

guccuaggcu cacaagaagg ugccguccac acagcucucg ccggagcccu agaagcagaa     1440

auggauggag caaaaggccg ccugucgagc ggccaccuca aaugucgacu aaaaauggac     1500

aaacuacgcc uaaaaggcgu cagcuacagc uuaugcacag cagccuucac cuucacaaaa     1560

auaccggcgg aaacacucca cggcacgguc acgguugaag uccaauacgc cggcacagac     1620

ggucccugca aagucccagc acaaauggca gucgacaugc aaacccuaac cccaguaggg     1680

cgucuuauca cagccaaccc agucauuacc gagucaacag aaaacaguaa aaugaugcua     1740

gaacucgacc cacccuuugg ggacucguac aucgucaucg gaauaggaga aaagaaaaua     1800

acccaccacu ggcaccgaag cggcuccacc auaggcaaag ccuucgaagc aacaguaaga     1860

ggagcaaaac gcauggcagu acuuggagac accgcguggg acuucggaag cgucggaggc     1920

gcgcucaaca gccucggcaa aggcauccac cagaucuucg gcgccgcguu caaaagccug     1980

uucggaggaa ugucaugguu cucccagaua cuaaucggaa cccuccuaau guggcuaggg     2040

cugaacacga aaaacggauc gauaagccua augugucucg cccuaggagg cguacuaaua     2100

uuccucucca cagcaguaag cgcagacguc ggcugcuccg uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9494
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-long mRNA Design 3 opt

<400>  9494
gggagaaagc uuaccaugag gauaauaaac gcacgaaaag agaaaaagcg aagaggagcc       60

gacacauccg ucggaaucgu cggacugcua uuaacaacag ccauggccgc agaagucacc      120

cgcagaggcu ccgccuacua cauguaccua gaccgaaacg acgcggguga agcaaucucc      180

uuccccacca cacucgguau gaacaaaugc uacauacaga ucauggaccu cggccacaug      240

ugcgacgcaa cgauguccua cgaaugccca augcuagacg aaggcguaga acccgacgac      300

gucgacugcu ggugcaacac aaccaguaca ugggucguau acgggaccug ccaccacaag      360

aaaggagaag cccgaagauc ccggcgggca guaacccuac cgucacacuc cacacgcaaa      420

cuucaaacgc gaagccaaac cuggcuagag aguagggaau auacaaaaca ccuaauaaga      480

guagagaacu ggauauuccg caaccccgga uucgcgcucg cggccgccgc aauagcaugg      540

cuacucggca gcagcaccag ccagaaagua auauaccuag ucaugauccu acucauagcc      600

ccggcuuacu caauccgcug cauagggguc agcaaccgcg acuucguaga aggcaugagc      660

ggaggaacau ggguggaugu aguacuagag cacggcgggu gcguaaccgu aauggcacaa      720

gacaaaccua ccgucgacau cgaacucgua agcacaacug uauccaacau ggccgaagua      780

aggucuuacu gcuacgaagc caccauaucc gacaucgccu ccgacuccag augcccaacg      840

caaggggaag ccuaccuaga caaacaaucc gacacacaau auguaugcaa acgaacccua      900

gucgacagag gauggggaaa cggcugcgga cuauucggca aaggaagucu gguaaccugc      960

gcaaaauucg caugcuccaa gaaaaugaca ggaaaaucca uacaacccga aaaccucgaa     1020

uaccgcauca ugcuaagcgu ccacggaagc caacacagcg gaaugaucgu caacgacacc     1080

ggacacgaaa ccgacgaaaa ccgagccaaa guagaaauca caccaaacag cccccgcgcc     1140

gaagccaccc ucggcggcuu cggcucccua ggacuggacu gcgaaccacg aaccggccua     1200

gacuucagcg accuauacua ccuaaccaug aacaacaaac acuggcuagu ccacaaagag     1260

ugguuccacg acaucccauu gcccuggcac gccggagccg acaccgguac accccacugg     1320

aacaacaaag aagcccuagu agaauuuaaa gacgcccacg cuaaaagaca aaccgcaguc     1380

guccucggau cccaagaagg cgccguacac acagcacuag caggcgcgcu cgaagccgaa     1440

auggauggcg cgaaaggaag acucagcagc gggcaccuca aaugccgguu aaaaauggac     1500

aaacuccgcc ucaaaggcgu cucauacagc cucugcaccg cagcuuucac cuucacuaaa     1560

auccccgcgg aaacucuaca cgggacagug accgucgaag uccaauacgc cggaacagac     1620

ggcccaugca agguaccagc acaaauggca gucgacaugc aaacccuaac cccgguagga     1680

agauuaauaa cggccaaccc uguaauaacc gaaagcaccg aaaacagcaa gaugaugcuc     1740

gaacucgauc ccccuuuugg cgacagcuac aucguaaucg gcgucggcga aaagaaaauc     1800

acccaccacu ggcaccgauc cggauccaca aucggcaaag ccuucgaagc aacgguccgg     1860

ggagccaaac gaauggcggu ccugggagac acggcauggg acuucggauc cguagguggg     1920

gcccuaaacu cguuaggcaa aggcauccac cagaucuucg gcgcagccuu caaaucacuc     1980

uucgggggaa ugagcugguu cucgcaaauc uuaaucggaa cacuucuaau guggcucggc     2040

cuuaacacca aaaacggaag caucucgcug augugccuag cacuaggcgg aguccucaua     2100

uuccuaagca cagccguauc cgcagacgug ggcugcagcg uugacuuuag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9495
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-long mRNA Design 3 opt

<400>  9495
gggagaaagc uuaccaugag aaucauaaac gcacgcaagg aaaagaaacg gcgcggcgcc       60

gacacauccg uaggcaucgu cggcuuacuc cuaacaacag caauggcagc cgaaguaaca      120

aggcgaggaa gugccuacua cauguaccug gacaggaacg acgcaggaga agccaucagc      180

uucccaacca cucucggcau gaacaaaugc uauauacaaa ucauggaccu aggacauaug      240

ugcgacgcaa ccaugagcua cgaaugcccc augcuagacg aaggagugga accagacgau      300

guagacugcu ggugcaacac aaccucaaca ugggucguau acggaaccug ccaccacaag      360

aaaggugaag cccgacgcuc aagacgagcg guaacacucc cuagccacuc uacccgaaaa      420

cuccaaaccc gcagccaaac auggcucgaa ucccgagagu acacaaaaca ccuaauacga      480

guagaaaacu ggauauuccg aaacccggga uucgcccuag ccgcagccgc aaucgccugg      540

cuccuaggca gcagcaccuc gcaaaaaguc aucuaccuag ucaugauacu acucauagcc      600

cccgccuaca gcauccgcug cauaggagua ucgaaccggg acuucguaga gggcaugagc      660

ggcggcaccu gggucgacgu aguacuagaa cacggcgggu gcguuacggu cauggcacaa      720

gacaaaccca cggucgacau cgagcuaguc acgaccaccg ucucaaacau ggcagaaguc      780

cgcuccuacu guuacgaagc aucaaucagc gacauggcgu ccgacuccag gugcccgacc      840

caaggagaag ccuaucucga caagcaaagc gacacacagu auguuugcaa gcgcacccuu      900

gucgacaggg gcugggguaa cggcugcggc cucuucggga agggcucccu agucacaugu      960

gcaaaauucg caugcagcaa gaaaaugacc ggcaaaucca uccaaccaga aaaccucgaa     1020

uaccgcauaa uguuaagcgu acacggcucc caacauuccg gcaugaucgu uaacgacacc     1080

ggacacgaaa cagacgaaaa ccgagcaaaa gucgaaauaa caccaaacag cccccgagca     1140

gaagccaccc uaggaggauu cggaucacuc ggacuagacu gcgaaccgcg aaccggacuc     1200

gacuucucag accuuuacua ccuaacaaug aacaacaaac acuggcucgu acacaaagaa     1260

ugguuccacg acauaccacu gcccuggcac gccggcgcag acacaggaac gccacacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg ccaagcgaca aacaguggua     1380

guacucggaa cccaagaagg ggccguccac accgcccugg caggagcacu cgaagccgaa     1440

auggacgggg cgaagggacg ccucuccuca ggacacuuaa aaugcagacu uaagauggac     1500

aaacuccgac uaaaaggagu cagcuacagc cuaugcaccg cagcauucac guucaccaaa     1560

auaccagcug aaacacucca cggcacgguc acagucgaag uccaauacgc cggcacagac     1620

ggaccaugca aaguacccgc ccagauggcc guggacaugc aaacgcucac ccccgucgga     1680

cgccucauaa ccgccaaccc cgucauaacc gaauccaccg aaaacagcaa aaugaugcua     1740

gaacucgacc cucccuucgg agacucauac aucgucauag gcgucggcga aaagaaaauc     1800

acgcaccauu ggcacagauc cggaucaaca auaggcaaag cauucgaagc aacagugaga     1860

ggggccaagc gaauggcagu ccuuggagac acagcauggg acuucggaag cguagguggu     1920

gcgcuaaacu cccuaggcaa aggaauccac caaaucuucg gagcagcauu caaaagccuu     1980

uuuggcggaa ugucgugguu cagccaaauc cuaaucggga cgcugcuaau guggcuagga     2040

cuaaacacga aaaacggauc caucucgcug augugccuag cacuaggcgg aguccugauc     2100

uuccuuucaa ccgccgucuc cgcagacgua ggcugcucag ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9496
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-long mRNA Design 3 opt

<400>  9496
gggagaaagc uuaccaugcg aauaaucaac gcccgaaaag aaaagaaaag acgaggggca       60

gacacauccg ucggcauagu agggcuccuc cucacaacag ccauggccgc agaaguaacc      120

cgacgaggca gcgcauacua uauguaccuc gacaggaacg acgccgguga ggcaauaucg      180

uuccccacga cccucgggau gaacaagugc uauauacaga ucauggaccu cggccacaug      240

ugcgacgcaa caaugucaua cgaaugucca augcucgacg agggaguaga acccgaugac      300

guugacugcu gguguaacac caccagcacg uggguugucu acggaaccug ccaucacaag      360

aaaggcgaag cccgacgcuc ccgcagagcc gucacacugc ccucgcauuc aacaagaaaa      420

cuccaaacac gcucccaaac auggcucgaa ucccgcgaau auacaaaaca ccucauccgc      480

guagaaaacu ggauauucag gaaucccggg uucgcccuag ccgccgcagc aaucgccugg      540

uugcucggcu caagcaccag ccaaaaagua auauacuuag ucaugauccu acucaucgcc      600

cccgcguaca gcauccgcug caucggaguc aguaaccggg acuucgucga aggcaugucc      660

ggaggaacau ggguggacgu cguacuagag cacggaggcu gcgugaccgu aauggcacaa      720

gacaaaccca caguagauau agagcuagua acgacaaccg uauccaacau ggcagaaguu      780

cgaagcuacu gcuaugaagc aagcaucagc gacauggcaa gcgacucccg augccccacc      840

cagggagaag ccuaccuaga caagcaaucc gacacacaau acguaugcaa acgaacccuc      900

gucgaccgcg gauggggaaa cggcugcggc cuauucggga aaggaucccu aguaacaugc      960

gcaaaauucg caugcagcaa gaaaaugacc gguaagagca uccaacccga aaaccucgaa     1020

uaccgaauaa ugcucagugu acacggcucc caacacagcg gcaugaucgu aaacgacaca     1080

ggacacgaaa ccgacgaaaa ccgugcgaaa guggaaauaa ccccgaacuc ccccagagcc     1140

gaagcaaccc uaggcggcuu cggaucacug ggccucgacu gcgaaccaag aaccggacua     1200

gacuucuccg accuauacua ccucacaaug aacaacaaac acuggcucgu acacaaggaa     1260

ugguuccacg acauaccccu ccccuggcac gccggagcag acacagggac accgcacugg     1320

aacaauaaag aagcccuggu cgaguucaaa gacgcucacg caaaacgcca aacugucguc     1380

guccuuggca gccaagaagg cgccguccac acagcacucg ccggugcccu cgaagccgaa     1440

auggacggag ccaaagggcg gcuaagcagc gggcaccuga aaugccggcu uaaaauggac     1500

aaacuccgac ugaaaggugu aucauacagc cuaugcaccg cagccuucac auucacaaaa     1560

auacccgcag aaacacucca cggaaccguc acagucgaac uccaauacgc cggaaccgac     1620

ggacccugca aagugccagc ccaaauggca guagacaugc aaacgcucac ucccguaggc     1680

cgccuuauaa cugcgaaccc cgucauaacc gaaagcacag aaaacagcaa aaugaugcuc     1740

gaacucgauc ccccguuugg ggacucauac aucgucauug gcgugggcga aaagaaaauc     1800

acgcaccacu ggcaccgcuc aggcagcacu aucggaaaag cauucgaagc aaccguccgc     1860

ggcgccaaga gaauggcugu acucggcgac accgcauggg acuucggauc ggucggaggg     1920

gcccucaaca gccucggcaa aggaauacac caaaucuucg gcgccgccuu caaaucucua     1980

uucggaggga ugucgugguu uucccagauc cugauaggca cccuccucau guggcuaggc     2040

cucaacacga aaaacggcuc cauuagccuu augugccucg cccuaggcgg cguauuaauc     2100

uuccucucaa cagcagucag cgccgacgua gggugcagcg ucgacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9497
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-long mRNA Design 3 opt

<400>  9497
gggagaaagc uuaccaugcg gaucaucaac gcccgcaaag aaaagaagcg cagaggagca       60

gaaaccuccg ucggcauagu cggccugcua cuaaccaccg ccauggccgc cgaagucacc      120

agacgaggcu ccgccuacua cauguaccuc gacagaaacg acgccggcga agccaucucc      180

uucccuacaa cccucggcau gaacaaaugc uacauacaaa uaauggaccu aggccacaug      240

ugcgacgcca cuaugagcua cgaaugcccc augcucgacg aaggagucga acccgacgac      300

guggauugcu ggugcaacac cacaucgaca ugggucgugu acgggacgug ccaccacaaa      360

aagggagaag ccagacgauc acgcagagcc gucacacucc ccagccacag cacgagaaaa      420

cuacaaacac guagccaaac guggcucgaa agccgcgaau acaccaagca ccucauccgc      480

aucgagaacu ggauauuccg caaccccggc uucgcacuag ccgcggccgc caucgcaugg      540

cuacucggau cgagcaccuc acaaaaaguc auauaccuag ucaugauccu acuaauagcc      600

cccgcauacu ccauacgcug cauuggcguc agcaaccggg acuucguaga aggcaugagc      660

gguggcacau gggucgacgu cguccucgaa cacggaggcu gcguaaccgu cauggcucaa      720

gacaaaccua cgguagacau agaacugguu accacaacag ucagcaacau ggccgaaguc      780

cgcagcuauu gcuacgaagc aagcauauca gacauggcca gcgacucccg uugcccaaca      840

cagggagagg cauaccuaga caaacaaucc gauacacagu acguaugcaa acguacccuc      900

gucgaccgcg guuggggcaa uggcugcggc cuauucggaa aaggaagccu cgucacaugu      960

gcaaaauucg ccugcaguaa gaagaugaca ggcaaaucaa uccaacccga aaaccuagaa     1020

uacagaauaa ugcucucggu ccacggaagc caacacuccg gaaugaucgu aaacgacacc     1080

ggacacgaaa ccgacgagaa ccgcgccaag gucgaaauca cccccaacuc accacgcgcg     1140

gaagcaacac ugggaggcuu cggaucacuc ggacucgacu gcgaaccccg aaccggauua     1200

gacuuuuccg accucuacua ccucacaaug aacaauaaac acuggcucgu ccacaaggaa     1260

ugguuccaug acauaccacu acccuggcau gccggcgcgg auacgggaac gccccacugg     1320

aacaauaagg aggcccucgu ggaguucaaa gacgcccacg caaaacgcca aaccguagua     1380

gugcucggca gccaagaagg agcuguccac accgcccugg ccggagcccu cgaagccgaa     1440

auggacgggg cgaaaggacg ccucuccucg ggccaccuga aaugccgccu aaaaauggac     1500

aaacuccgau uaaaaggggu caguuacagc cuaugcaccg cggcauucac cuucacaaaa     1560

auaccagcag aaacacuaca uggaaccguc acagucgaag uccaguacgc cggcacagac     1620

gguccaugua aaguccccgc acaaauggcc gucgacaugc aaacacugac uccagucgga     1680

agacucauca ccgcaaaccc aguaauaacc gaaucaaccg aaaacuccaa aaugaugcuc     1740

gaacucgacc caccauucgg cgacuccuac auaguaauag gcgucggaga aaagaaaaua     1800

acacaccacu ggcaccgaag cggauccacc auuggcaagg cuuuugaagc aaccguacga     1860

ggagccaaac gcauggccgu gcuaggagac accgccuggg acuucggcuc cgucggcggc     1920

gcccuaaacu cccugggcaa aggcauacau caaauauucg gcgccgcauu caaaucacuc     1980

uucggaggca ugagcugguu cagccagauc cucauaggca cccuccuuau gugguugggc     2040

cucaacacca agaacggaag caucucccuc augugccuag cacucggcgg cguacucaua     2100

uuccucucaa cagccgucag cgccgacgua ggcugcagcg ucgacuucuc uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9498
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-long mRNA Design 3 opt

<400>  9498
gggagaaagc uuaccaugag aauaauaaac gcacgaaagg aaaagaaacg ccgaggcgcg       60

gacacaagcg ugggaauagu cggacuccua cucacaacgg caauggcagc cgaagucaca      120

cgaaggggcu cagcuuacua cauguaccug gacagaaacg acgccgggga agcaaucagc      180

uuuccgacca cacuaggaau gaauaaaugc uacauacaaa uaauggaccu cggacacaug      240

ugcgaugcaa ccaugucgua cgaaugccca augcuugacg agggcguaga gcccgacgac      300

gucgauugcu gguguaacac cacgagcaca uggguaguuu auggaacaug ccaccacaag      360

aaaggagaag cacgcagaag cagacgcgcu gucacgcucc caagucacag cacgcgcaag      420

cuccagaccc ggagccagac cugguuagaa agccgcgaau acaccaaaca ccucaucaga      480

gucgaaaacu ggaucuucag aaacccaggg uucgcacuag cagccgccgc cauagccugg      540

cuacucggau ccuccacgag ccagaaagua auauaccucg uaaugauacu ccugaucgca      600

ccagcauacu cgauccgcug cauaggcgua agcaaccggg acuucgugga agggauguca      660

ggaggaaccu gggucgaugu aguucuugag cacggaggau gugugacggu aauggcucag      720

gacaaaccca cgguagacau ugaacucguc accaccaccg ugagcaacau ggcagaagug      780

cgaucauacu gcuacgaggc cuccauaucc gacauggcca gugacucaag augcccaacg      840

caaggagaag cguaccuaga caaacaauca gacacgcagu acgucugcaa acgaacgcua      900

gucgaccgcg gaugggggaa cgggugcgga cuauuuggca aaggcucacu ggugacaugc      960

gccaaauuug caugcucaaa gaaaaugaca ggcaaaucca uacaacccga gaaccucgaa     1020

uaucggauca ugcuauccgu acauggcuca caacauagcg gcaugauagu caacgacaca     1080

ggacacgaaa cagacgagaa cagagccaaa gucgaaauaa ccccaaauag cccccgcgcg     1140

gaagcaaccc ucggaggauu cggaagccua ggacuggacu gugaaccaag aacaggcuug     1200

gacuuuagcg accucuacua ccugacaaug aacaacaaac acuggcuagu ccacaaagaa     1260

ugguuccacg acauaccgcu accguggcac gcaggggccg acaccggcac cccgcauugg     1320

aacaacaaag aggccuuagu cgaguucaaa gacgcacacg caaaaagaca aacgguagua     1380

guacucggcu cgcaagaagg cgccguacac accgcgcucg caggagcccu cgaagcggaa     1440

auggacggag caaaaggucg ccugagcucc ggccaucuca aaugcagacu aaaaauggac     1500

aaacugagac ucaaaggggu aucuuacagc cuaugcacgg cagccuucac auucacaaaa     1560

aucccugccg agacacuaca cgggacgguc acagucgagg uacaauacgc cggaaccgac     1620

ggaccaugca agguacccgc ccaaauggcc gucgacaugc aaacacuaac accagucggg     1680

cgccuaauca cagcuaaucc cgucaucacu gaaucaaucg aaaacagcaa aaugaugcua     1740

gaacuagauc cgcccuucgg cgacagcuac aucguaaucg gcgucggaga aaagaaaauc     1800

acacaccacu ggcauagaag cgggagcaca auaggcaaag cauucgaagc cacaguacgc     1860

ggagcgaaac gaauggcagu ccucggcgac accgcguggg auuucgggag cgucggcggc     1920

gcacucaacu cgcucggaaa aggaauccac caaaucuucg gagccgcauu caaaagccua     1980

uuuggcggca ugagcugguu uagccaaaua cucaucggaa cacuccugau guggcucgga     2040

cucaacacga aaaacggcag cauaaguuug augugccucg cccucggagg gguacucaua     2100

uuucugagca cagccgucag cgcagacgua gggugcagcg uggacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9499
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-long mRNA Design 3 opt

<400>  9499
gggagaaagc uuaccaugcg caucauaaac gcccgcaaag aaaagaaacg cagaggcgca       60

gacacaucag ucggcaucgu aggacuucua cuaacuaccg cgauggcagc agagguaacc      120

cgccgcggau ccgcauauua cauguaccuc gaccgcaacg acgcgggcga agcaaucuca      180

uucccaacaa cccugggaau gaacaaaugu uacauccaaa ucauggaccu cggacacaug      240

ugcgacgcaa ccauguccua cgaaugcccc augcuagacg aaggcgucga accagacgac      300

gucgacugcu ggugcaacac caccagcaca uggguuguau acggcacgug ucaucacaag      360

aaaggagaag cgcgccgaag ccgacgcgca gugacgcugc ccucccacuc cacccgcaaa      420

cugcaaacgc gcucccagac auggcuagag agccgagagu acaccaaaca ccuaauccga      480

guagaaaacu ggaucuuccg caacccaggc uucgcacucg ccgccgccgc aaucgccugg      540

cuacucggau cuagcaccag ccaaaaagua aucuaccucg ucaugauccu ccugaucgcu      600

cccgccuaca gcauccggug caucgggguc agcaacagag acuucguaga aggaaugucc      660

gggggaacau ggguagacgu cguccuagaa cacgggggcu gcguaacugu cauggcucag      720

gacaaaccca cagucgacau agaacuuguc accaccaccg ucagcaauau ggcggaagua      780

cgcagcuacu guuacgaagc aagcauauca gacauggccu cugacagccg uugcccgacc      840

caaggagaag cauaucucga caaacagucc gacacccaau acgucugcaa acgaacccuc      900

guagaccgag gaugggggaa cggaugugga cucuucggca aaggaagccu cguaaccugc      960

gcaaaauucg cauguuccaa gaaaaugacc ggcaaaucaa uccaaccaga aaaccucgaa     1020

uaccgaauca ugcucagcgu ccacggaucc cagcacuccg gcaugaucgu aaacgauacu     1080

ggccacgaaa ccgacgaaaa ccgagccaaa gucgaaauaa cccccaauag cccacgcgcc     1140

gaagccaccc uaggcggauu cggcucccuc ggccucgacu gcgaaccacg caccggccua     1200

gacuucagcg accucuauua ccuaaccaug aacaacaaac acuggcuggu ccacaaagaa     1260

ugguuccacg acaucccccu cccuuggcac gccggggcgg acacaggaac cccacacugg     1320

aacaauaaag aagcacucgu cgaguucaaa gacgcacacg cuaaaaggca aaccgucgua     1380

guacuaggca gccaagaagg agcaguucac accgcauuag ccggcgcacu agaagccgaa     1440

auggacggag ucaaaggacg ccucuccucc ggacaccuaa aaugccgccu caaaauggac     1500

aaacuacgcc uaaaaggagu aaguuauuca cucugcacag cagccuucac auucaccaaa     1560

aucccagcag aaacccuaca cggcacagug acaguggaag uccaauacgc aggcaccgac     1620

ggcccaugca aagucccugc acagauggcc gucgacaugc aaacccucac accagucggc     1680

aggcucauca ccgcuaaccc aguaaucacc gaaucaaccg aaaacucaaa gaugaugcuc     1740

gaacucgacc cgcccuuugg ggacuccuac aucgugaucg ggguugggga aaagaaaauc     1800

acccaccacu ggcaccguuc aggaucaaca aucggcaaag cauucgaagc aaccguacga     1860

ggcgcaaaaa gaauggccgu acuaggcgac acagcauggg acuucggcuc agucggcggg     1920

gcccuaaacu cccucggcaa aggcauacac caaaucuucg gagcagcauu caagucccuc     1980

uucggcggca ugagcugguu cagccaaauc cucaucggca cacugcuaau guggcuggga     2040

cucaacacca aaaacggauc gaucucauuc augugccugg cacucggugg cgugcucaua     2100

uuccucagca cagccgucuc cgcagacguc ggcugcagcg uagauuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9500
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-long mRNA Design 3 opt

<400>  9500
gggagaaagc uuaccaugag gauaauaaac gcccgaaagg aaaagaaacg acgaggagcc       60

gacacauccg ucggcaucgu aggacuccuc cuuaccaccg ccauggcggc ggaggucaca      120

cgcagagggu cagccuacua cauguaccua gaccgcaacg acgccggcga agcgaucagu      180

uuucccacca cgcugggaau gaacaaaugc uacauccaaa ucauggaccu aggacacaug      240

ugcgacgcca caaugucaua cgaaugcccc augcucgacg aaggcgucga accagacgac      300

gucgacugcu ggugcaacac gaccuccaca ugggucgugu acggaaccug ucaccacaag      360

aaaggcgaag cccgacgauc acgcagagcc gucacccuac ccucccacag cacccgcaaa      420

cuucaaacca gaucacaaac cuggcucgaa ucccgagagu auacaaaaca ccucauacga      480

gucgaaaacu ggaucuuccg caacccagga uuugcccugg cggccgcugc cauagccugg      540

cuacucggcu ccucaaccuc acaaaagguc auauaccucg ucaugauccu ccuaaucgcc      600

cccgccuaca gcauccgcug uauaggaguc ucaaacagag acuuuguaga aggaauguca      660

ggcgggaccu ggguugacgu gguacuggaa cacggcggcu gcguaacugu cauggcgcaa      720

gacaaaccca caguggacau agagcuagua accaccaccg uaagcaacau ggcagaagua      780

cgcuccuacu gcuacgaagc aucgaucucc gacauggccu cagacucccg cugccccacc      840

caaggcgaag cauaccucga caaacaauca gacacccaau acgucugcaa gcgcacccuc      900

guagaccgcg gauggggcaa cggcugcggc cuguucggga aaggcucccu aguaaccugc      960

gccaaauucg caugcagcaa aaagaugacu ggcaaaucca uccaacccga gaaccucgaa     1020

uacagaauca ugcucucagu ccacggaucc caacacuccg gcaugauagu caaugacacg     1080

ggacacgaaa cagacgaaaa ccgcgcgaaa gucgagauca ccccaaacuc accccgagca     1140

gaagcaaccc uuggaggauu cggcagccua ggacucgacu gcgaacccag aaccggccua     1200

gacuucuccg accucuacua ucucaccaug aacaacaaac acugguuagu ccauaaggaa     1260

ugguuccacg acaucccccu ccccuggcac gcaggcgcug acaccggcac accccacugg     1320

aacaacaaag aggcacucgu cgaguucaag gacgcccacg caaaacgaca aacaguaguc     1380

guacucggcu cccaggaagg cgcaguacac acagcccuag caggcgcacu cgaagcggaa     1440

auggacggag caaaagguaa acuuucaagc ggccaccuca aaugccgacu gaaaauggac     1500

aagcugcgcc uaaaaggagu cagcuacagu cucugcacgg ccgcauucac auuuacaaaa     1560

auaccagccg aaacgcuaca cggcaccguc accgucgaag uccaauacgc cggcaccgac     1620

ggccccugca aaguacccgc acaaauggcc guagacaugc aaacccucac accgguagga     1680

cgccucauca ccgcaaaccc cgucauuaca gaauccaccg aaaacucaaa aaugaugcuc     1740

gaacuagauc ccccguuugg ugacuccuac aucgucaucg gaguaggaga aaagaaaaua     1800

acacaccacu ggcaccgcag cggaucgaca aucggaaaag ccuucgaggc aaccgugcgc     1860

ggcgcaaaac gaauggccgu ccucggagac accgccuggg acuucggcuc cgucggcggg     1920

gcacuaaaca gccuaggaaa aggaauccau cagauauucg gagcagccuu caaaucacug     1980

uucggaggca uguccugguu cucccaaaua cugauaggca cacuacucau gugguuaggc     2040

cucaacacca aaaacggaag cauaagccuc augugccucg cccucggcgg cguccucaua     2100

uuccucucca cagcggucuc cgcagacgug ggaugcagcg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9501
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-long mRNA Design 3 opt

<400>  9501
gggagaaagc uuaccaugcg aaucaucaac gcacgcaaag aaaaaaaacg gcgaggcgcc       60

gacaccagcg uaggcauagu aggacuccua cuaacaacag ccauggccgc cgaaguaacc      120

cgacgcggcu ccgccuacua cauguaucuc gaccggaacg acgccggcga agcaauaucc      180

uuccccacaa cccucggcau gaacaaaugc uacauacaaa ucauggaccu cggccauaug      240

ugcgacgcaa caaugucaua cgaaugcccc augcucgacg aaggcguuga gccagacgac      300

gucgacuguu ggugcaacac cacaagcacc uggguagucu acggaacaug ccaccauaaa      360

aaaggcgaag ccagacgauc caggagagcg guaacucugc caucacacuc cacacgaaaa      420

cuacaaacca gaucccaaac auggcucgaa agccgcgaau acaccaagca ccucauccgc      480

guagagaacu ggauuuuccg aaaccccgga uucgcccuag ccgccgccgc caucgccugg      540

cuucugggau ccucgaccag ccaaaaagua aucuaccucg ucaugauccu cuuaauagcc      600

cccgcguaca gcauacgaug caucggagua ucgaaccgcg acuucguaga gggaaugagc      660

ggcggcacau ggguagacgu aguacucgag cacggagguu gcguaacagu cauggcacaa      720

gacaaaccua caguggacau cgaacucgua acgaccaccg ucagcaacau ggcggaagug      780

cgcucauacu gcuacgaagc aucgauaagc gacauggccu ccgacucccg cugcccaacg      840

caaggcgaag ccuaccucga uaaacagucc gacacccagu acgucugcaa acgcaccuua      900

gucgacaggg gauggggaaa cggcugcggc cuauucggca aaggcagccu ugucaccugc      960

gccaaauucg cgugcagcaa aaaaaugaca ggaaaaucca uccaacccga aaacuuagag     1020

uaccgcauaa uguuaucagu ccacggaucc caacacucag gaaugaucgu caacgauaca     1080

ggccacgaaa cagacgaaaa ccgcgccaaa gucgaaauca cacccaacuc cccccgcgca     1140

gaagccaccc uaggcggguu cggaagccuc ggacuagacu gcgagccccg cacaggacuu     1200

gacuucagug accuauacua ccuaacaaug aacaacaagc acuggcucgu ccacaaagaa     1260

ugguuccacg acaucccccu acccuggcac gcaggagccg auaccggcac accucacugg     1320

aauaacaaag aagcgcuagu cgaauucaaa gaagcacacg caaaacgaca aacaguagua     1380

guguuaggcu cacaagaagg cgcaguccac accgcgcuag ccggagcccu agaagcggaa     1440

auggacggcg caaaaggccg ccuaucaagc ggccaucuaa agugucgacu aaaaauggac     1500

aaacuccgac ugaaaggcgu auccuauagc cugugcaccg ccgcauucac cuucacaaaa     1560

auccccgcag agacccuuca cggaacgguc acuguagaag uccaguacgc aggcaccgac     1620

ggaccaugca aaguccccgc acaaauggcc guugacaugc aaacccuaac accagucgga     1680

agacucauaa ccgccaaccc agucaucaca gaaaguaccg aaaacagcaa aaugaugcuc     1740

gaacucgacc ccccguucgg cgacagcuac aucgugaucg gcguaggcga aaaaaaaaua     1800

acgcaccacu ggcauagauc aggcagcacc auaggaaaag ccuucgaagc aacaguacgc     1860

ggcgcaaaac gaauggccgu acucggcgac acggcauggg acuucggcuc cgugggcgga     1920

gcgcugaaua gucugggcaa agguauccac caaaucuuug gggccgcauu uaaaucccuc     1980

uucggcggca ugucaugguu cucacaaauc cuaauaggaa cacuucugau guggcuggga     2040

cucaacacga aaaacggcuc caucucccug augugccuag cacucggcgg cguacugaua     2100

uuccuaucca cagcagucuc agcagacgua ggcugcucgg ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9502
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-long mRNA Design 3 opt

<400>  9502
gggagaaagc uuaccaugcg aaucaucaac gcaaggaaag aaaagaaaag acggggcgcg       60

gacacaagcg ucgggaucgu cggucuacua cucacaaccg caauggccgc ugaagucacc      120

cgcagaggca gcgcauauua cauguaccug gacagaaaug acgcaggaga ggcaaucagc      180

uuccccacaa cgcuaggcau gaacaaaugc uacauacaaa ucauggaccu aggacacaug      240

ugcgaugcga caaugagcua ugagugcccc augcucgacg aaggagucga accagacgau      300

gucgacugcu ggugcaacac cacaagcacg ugggucguau acggcacaug ccaccacaaa      360

aagggcgaag cacgacgaag cagacgagcc gucacacugc caucacacuc cacacgcaaa      420

cuccaaacac ggagccaaac auggcuggaa ucccgggaau acaccaaaca ccucauccgg      480

gucgaaaacu ggauauucag aaacccagga uucgcccucg cagcugccgc cauagcaugg      540

cuccuaggcu caagcacauc acaaaaagug auauaccuug uaaugauacu acucaucgcg      600

cccgccuacu caauacgaug cauaggggua agcaaucgcg auuucgugga agguauguca      660

gguggcaccu ggguagacgu cgugcucgag cacggaggau guguaacggu aauggcacag      720

gacaaaccca cggucgacau cgagcucguc accacgacgg uaagcaacau ggccgaagua      780

cgcucauacu gcuacgaggc guccauauca gacauggcau cagacagccg cugccccacc      840

cagggagagg cauaccugga caaacaaagu gauacacaau acguaugcaa acggacgcuc      900

gucgacagag gcuggggcaa cggaugugga cuauucggca aaggaagccu aguaacgugc      960

gccaaauucg ccugcagcaa gaaaaugaca ggaaaaucca uacaaccaga aaaccuagaa     1020

uaccgaauaa ugcucucagu acacggcuca caacacagug gcaugauagu aaacgacacc     1080

ggccaugaaa cugacgaaaa ccgcgccaaa gucgaaauca cccccaacag cccgcgagca     1140

gaagcaacac ucggaggauu cggauccuua ggacucgacu gcgaaccacg cacaggacuc     1200

gauuucagcg accucuacua ccucacgaug aacaauaaac auuggcuagu ccacaaagaa     1260

ugguuccacg acaucccccu acccuggcac gcaggcgccg acacaggaac accccacugg     1320

aacaacaaag aagcacuggu agaguucaaa gacgcacacg ccaaacgcca gacagucgug     1380

guccuaggau cacaggaggg ggcaguccac accgcacucg ccggagcccu agaagccgag     1440

auggacggag ccaaaggaag acucagcucg ggccaccuca aaugcagacu aaaaauggau     1500

aaacuaagac ucaaaggugu cuccuacucc cucuguacag ccgccuucac auucaccaaa     1560

auaccagccg agacgcuaca cggcaccgua acagucgagg ugcaguacgc aggcgcggac     1620

ggaccaugca aggugccagc ccaaauggcc guagacaugc agacccucac accgguaggc     1680

cgacuaauaa ccgccaaucc cguaauaacc gaauccaccg aaaacucaaa aaugauguua     1740

gaacuggacc cgcccuucgg ggacagcuac auagucaucg gagugggaga aaagaaaauc     1800

acacaccacu ggcacagaag uggaagcaca auagggaaag cguuugaggc caccguacgc     1860

ggcgccaaac gcauggcagu ccuaggagac accgcauggg acuucggcag cgucggcgga     1920

gcacucaacu cacugggcaa gggaauccac caaauuuucg gcgcagcauu caaaagccua     1980

uucggaggaa ugagcugguu cucacaaaua cugaucggca cacuacuaau guggcuggga     2040

cugaauacca aaaacggaag caucaguuua augugccuug cacuaggagg cguacugaua     2100

uuccuaagca cagccgucuc cgcagacgug gggugcagcg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9503
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761560.1 prME-long mRNA Design 3 opt

<400>  9503
gggagaaagc uuaccaugag aauaauaaac gcagggaaag agaagaaacg aaggggagcc       60

gacacaaacg ucggcaucgu cggacuauug cuaacaacgg cgauggcagc cgaaguaaca      120

cgccgcggau cagcguacua cauguaccug gaccgaaacg acgccggaga agcaaucucc      180

uucccaacca cgcucggcau gaacaaaugc uacauccaaa uaauggaccu aggacacaug      240

ugcgacgcca ccauguccua cgaaugcccg augcucgacg aaggcgucga accagacgau      300

gucgauugcu gguguaauac cacgaguaca ugggucgucu acggcacaug ccaucacaag      360

aaaggagaag cacgcagaag cagacgcgcc guaacacuac caagccauuc cacaagaaaa      420

cuacagacaa ggagccaaac auggcuggaa agccgcgaau acacaaaaca ccuaaucaga      480

guggaaaacu ggaucuucag gaauccagga uucgcacugg ccgcggcggc aauagcgugg      540

cuccuaggaa gcaguacauc acaaaaagua aucuaccucg uaaugauauu gcugaucgcg      600

ccagccuacu cuauaaggug cauaggaguc agcaaccgug acuucguaga aggaaugucc      660

ggcggcaccu gggucgacgu cgugcucgaa cacggagggu gugucaccgu aauggcccaa      720

gacaaaccca ccguagacau agaacugguc acaacgaccg uaagcaacau ggcagaagug      780

cgcagcuacu gcuacgaggc cucaaucagc gacauggcaa gcgacucccg cugcccaaca      840

caaggcgaag cauaccugga caaacagagc gacacccagu augucugcaa aaggacccuc      900

gucgaccgag gauggggaaa cggaugcggc cuauucggca aaggaucccu agugacgugc      960

gccaaauucg caugcaguaa gaaaaugacc ggcaaaagca uacaaccaga aaacuuagaa     1020

uacaggauca ugcucucagu gcacggauca caacacagcg gaaugauagu caacgacacc     1080

ggccacgaaa cggacgaaaa cagagccaaa gucgaaauua cacccaacag uccacgcgca     1140

gaagcgaccc ucggaggguu cgggagcuua ggacucgacu gcgaaccacg aacgggacuc     1200

gacuucagcg accuguauua ccuaacaaug aacaacaaac auuggcuagu gcacaaagag     1260

ugguuccacg acaucccccu accauggcac gccggggccg acacaggcac accccauugg     1320

aacaacaaag aagcucuagu cgaguucaaa gacgcacacg caaaaaggca aaccgucgug     1380

guacuuggca gccaagaagg cgcaguccac acugcacuag caggagcgcu agaagccgaa     1440

auggacggag caaaaggccg ccuauccucg gggcaccuca aaugcagauu gaaaauggac     1500

aaacuaagac ugaaaggagu aucauacucc cucugcaccg cugccuucac cuucaccaaa     1560

auacccgcag aaacccucca cggaacggua acagucgaag uacaguaugc cggaaccgac     1620

ggccccugca aggucccagc ccaaauggcc gucgacaugc aaacacuaac cccaguuggc     1680

cgccuaauca cggccaaccc aguaaucaca gaaucaaccg agaacuccaa aaugaugcuc     1740

gaacucgacc cacccuucgg agacagcuac aucguaaucg gcgucggcga aaagaaaaua     1800

acccaccacu ggcaccgcag cggaagcaca auuggcaaag cguucgaagc aacggucaga     1860

ggcgcacgca gaauggcagu ucucggcgau acagcauggg auuucggcag cguaggaggg     1920

gcgcucaacu cccucgggaa aggcauacau caaaucuuug gggcagcguu caaaagccug     1980

uucggcggca ugucaugguu cagucaaauc cuaauaggaa cacuccuaau guggcucggg     2040

cuaaacacga aaaacggguc caucagcuua augugccucg cacucggagg aguacucauc     2100

uuccuaucca cagcuguaag cgcagacguc ggaugcucag uggacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9504
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-long mRNA Design 3 opt

<400>  9504
gggagaaagc uuaccaugcg cauaaucaac gccagaaaag agaagaaacg acgaggagca       60

gaaaccagcg uggggauagu gggccugcuc cuaaccacag caauggcugc cgaagugaca      120

cgaagaggaa gcgccuauua cauguaccug gacagaaaag acgcaggaga agccaucagc      180

uuuccaacca cacucgggau gaacaaaugc uacauccaga uaauggaccu aggccacaug      240

ugcgacgcaa ccaugucaua cgaaugcccc auguuagacg aaggcgugga gccggacgac      300

guagacugcu ggugcaacac caccagcacu uggguggugu acggaacaug ccaccacaag      360

aaaggcgaag ccagacgcag cagacgagcc gucacgcucc ccagccacuc cacacggaaa      420

cuacagacac gaagccaaac auggcuagaa agccgcgaau acaccaaaca ccuaaucaga      480

gucgagaacu ggaucuuccg caaucccggc uucgcauuag ccgcagccgc aauagccugg      540

cuacuaggca gcagcacauc ccaaaaaguu aucuaucuug ugaugauacu gcuaauagcc      600

cccgcguacu caauccggug caucggcguc ucaaaccggg acuucguaga aggcauguca      660

ggcggcaccu gggucgacgu cguacuagaa cauggaggau gcgucaccgu aauggcccaa      720

gacaaaccca ccguagacau cgaacucguc acgacuaccg ucaguaacau ggcagaagug      780

cgauccuacu gcuacgaagc cagcaucucc gacauggccu ccgacagccg cugcccaaca      840

caaggcgaag cauaccucga caaacaaagc gacacacaau acgugugcaa acgaacccua      900

gucgaccgcg gaugggguaa cggaugcggc cucuucggaa agggcagccu agugaccugc      960

gcaaaguucg caugcagcaa gaaaaugaca ggcaaaagua uccagccaga aaaucuggag     1020

uacagaauaa ugcuuucagu acacggauca caacacucag gaaugaucgu caacgacaca     1080

ggccaugaaa cagacgagaa ccgcgcgaaa guagaaauaa cccccaacag ccccagagca     1140

gaagcaacac ucggaggguu cggcagccua ggacucgacu gcgaaccacg aaccgggcua     1200

gacuucagcg accuauacua cuuaacaaug aacaauaaac acuggcucgu ucacaaagaa     1260

ugguuccacg acaucccccu accauggcau gcaggagccg acaccggaac gccccacugg     1320

aacaacaagg aggccuuagu agaguucaag gacgcacacg cgaagcgaca aaccguaguc     1380

guccuaggau cccaagaagg agccguacac acagcacugg ccggcgcccu cgaagcagaa     1440

auggacgggg caaaaggccg acuaagcagc ggccaccuaa agugcagacu gaagauggac     1500

aaacugagac ucaaaggcgu cuccuacagc cucugcaccg ccgcauucac auucaccaag     1560

auaccagcgg aaacacuaca cggaaccguc accguagaag ugcaauacgc aggcacugac     1620

ggccccugca aagucccagc acaaauggca guagacaugc aaacacucac accaguagga     1680

cgacucauca ccgccaaccc aguuaucacc gagucaacag aaaacucaaa aaugaugcuc     1740

gaacucgacc cgcccuucgg cgacagcuac aucguaauag gagugggaga aaagaaaaua     1800

acgcaccacu ggcaccggag cggcagcaca auaggaaaag cauucgaagc aacagucaga     1860

ggcgccaaaa gaauggccgu ccucggcgac acggcauggg acuucgggag cgucggaggc     1920

gcauuaaacu cccuggggaa aggaauccac caaauuuucg gagccgccuu caaaagccua     1980

uucggcggga ugucaugguu cagccaaauc cucauuggga cccuacuaau guggcuaggc     2040

cugaacacga agaacggaag cauaucacua augugccuag cacuaggggg aguccuaaua     2100

uuccuaucca cggcgguaag cgcagacgua ggaugcuccg uagacuucag caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9505
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-long mRNA Design 3 opt

<400>  9505
gggagaaagc uuaccaugcg aaucaucaac gcgagaaaag aaaagaaaag acguggagcg       60

gacacguccg ucggaaucgu cggacuauug cuaacgacgg cuauggccgc ggaagucaca      120

cgcagaggca gcgccuacua cauguaccuc gaccgcaacg acgcuggcga agcgaucagc      180

uuccccacca cccuuggaau gaacaagugu uacauccaga uaauggacuu aggacacaug      240

ugcgacgcca ccauguccua cgaaugcccu augcuugacg aaggcgucga gccagacgac      300

guggauugcu ggugcaacac aaccagcaca uggguaguau acggaaccug ccaucacaag      360

aaaggcgaag cccgaaggag uagacgagcc guaaccuuac caucgcacuc aacacgaaaa      420

cuacaaaccc gcucccaaac auggcuagaa ucccgcgaau acacaaaaca ccucauccgc      480

gucgaaaacu ggauauuccg caaccccgga uucgcacuag ccgccgccgc aaucgccugg      540

cuacucggaa gcucaaccuc gcaaaaaguc auauaccucg uaaugauacu gcuaauagca      600

ccggcauacu ccauacgaug uaucggcgug uccaacaggg acuucgucga aggaaugucc      660

ggaggaaccu gggucgacgu gguacuagag cacggaggcu gcgucacagu gauggcccag      720

gacaaaccaa cagucgacau agagcuaguc acaacaaccg uguccaacau ggccgaagua      780

agguccuacu gcuacgaagc cagcauaagc gacaugguau cagauucgcg augccccacc      840

caaggcgaag cauaccucga caaacaaagc gacacccagu acguaugcaa acgcacgcuc      900

gucgacagag gauggggcaa cggaugcggu uuauucggca aaggaagccu aguaaccugc      960

gcaaaauucg ccugcaguaa gaaaaugacc ggaaaaucca uacaaccaga aaaccuagaa     1020

uaccgcauca ugcucuccgu ccacggauca caacacagcg gaaugauagu caacgacacg     1080

ggacacgaaa ccgacgaaaa cagggccaaa guagaaauaa ccccaaacag uccccgugca     1140

gaagcaacac ucggcggguu cggcucacuc ggccucgacu gcgaaccaag aaccggccua     1200

gacuucuccg accucuacua ccuaacaaug aacaacaaac acuggcucgu acacaaagaa     1260

ugguuccacg acauaccccu accauggcac gccggcgccg acaccggcac uccacacugg     1320

aacaacaagg aagcccucgu agaguucaaa gacgcacacg cuaaacgcca aaccgucgua     1380

guccucggaa gccaggaagg agccguccac accgcccugg caggggcccu ggaagcugaa     1440

auggacggag caaaaggcag acucucaagc ggccaccuaa agugccgacu caaaauggac     1500

aaacucagac uaaagggagu aagcuacagc cucugcacag ccgcauucac auucaccaaa     1560

aucccagccg aaacucuaca cggcacaguc accguagaag uacaauacgc cggaacagac     1620

ggaccgugua aaguaccagc ucaaauggcc guggacaugc aaacacugac acccgucgga     1680

agacucauaa ccgccaaccc agucauaacc gaaucgaccg aaaacagcaa gaugaugcua     1740

gaacuagacc cgcccuucgg agacagcuac aucguaaucg gcguagggga aaagaaaaua     1800

acccaccacu ggcacagauc aggcuccaca auaggaaaag ccuucgaagc cacaguacga     1860

ggcgccaaac gaauggcugu auuaggugac acggcauggg acuuuggauc aguaggcggc     1920

gcccuaaacu cacuaggaaa aggcauccac caaaucuucg gggcagccuu caagucgcuc     1980

uuugggggca uguccugguu cucgcaaauc cucaucggga cccuccuaau guggcuagga     2040

cucaacacca agaacgguuc gauaucccug augugccucg cucucggagg aguccucauc     2100

uuccucucca cugccgucag cgccgacguu ggcugcucgg ucgauuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9506
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-long mRNA Design 3 opt

<400>  9506
gggagaaagc uuaccaugag aaucauaaac gcaagaaaag aaaagaaaag aagaggcgcg       60

gauaccaaug ucggaaucgu gggacuccua cuaacaacgg caauggcagc ggaaguaacc      120

cgacgaggca gcgcauacua cauguaccuc gacagaaacg acgcaggaga agcaaucagc      180

uuccccacca cucuaggcau gaacaaaugc uacauccaaa uaauggaccu cgggcacaug      240

ugcgacgcga caauguccua cgaaugcccc augcuggacg aaggcguaga acccgacgac      300

gucgacugcu ggugcaacau gacgagcaca uggguaguau acgggacgug ccaccacaag      360

aaaggcgaag cacgacgaag ccgccgggca guaacccucc caagccacag cacgcgcaaa      420

cuacagacuc gcucacaaac cuggcucgag agccgagaau acacaaagca ccuaauccgc      480

guagaaaacu ggaucuuccg aaaccccggc uucgcacuag cagcagcagc gauagcgugg      540

cuacugggcu caagcaccag ucagaaaguc aucuaccugg uaaugauacu acuaauagca      600

ccagccuaca gcaucaggug cauaggcgua agcaaccgag acuucguaga aggaaugagc      660

ggaggcaccu ggguggacgu cguucucgaa cacggagggu gcgucacagu aauggcccaa      720

gacaaaccca caguggacau cgagcuagua acaaccacag ucagcaacau ggcagaaguc      780

cgcucauacu gcuacgaagc cuccaucucc gacauggcca gugacucacg cugcccaaca      840

caaggagaag ccuaccucga caaacaauca gacacacagu acgucugcaa acgcacacug      900

gucgaccgcg gauggggaaa ugggugcgga cuauucggaa aaggaucccu cguaaccugc      960

gccaaauucg ccugcaguaa gaaaaugaca ggcaaaucaa uccagccgga aaaccuagaa     1020

uaccgcauca ugcucagcgu ccacggcagc caacacagcg guaugauagu aaacgacaca     1080

ggacacgaaa cagaugaaaa ccgcgcaaaa gucgagauca cacccaacag cccccgcgcc     1140

gaagcaaccc uagguggguu uggcagccug ggacuagacu gcgaaccacg aaccggacuc     1200

gacuucuccg accuauacua ccuaacuaug aacaauaaac acuggcucgu acauaaggaa     1260

ugguuccacg acaucccacu accauggcac gcuggagccg acaccggaac cccgcacugg     1320

aacaacaaag aagcgcucgu agaguucaaa gacgcacacg ccaaacgaca aacagucguu     1380

guacucggua gccaggaagg agcaguacac acagcacucg ccggagcacu agaagcagaa     1440

auggacggag caaaaggacg acugagcagc ggccaccuga agugcagacu caaaauggac     1500

aagcuccgcc ucaaaggagu cagcuacagc cucugcaccg ccgcguucac cuucacaaaa     1560

auaccagcgg aaacacuuca cggaacggua acaguggaag uacaauacgc cggaacagac     1620

ggacccugca aagucccagc ccagauggcc gucgacaugc aaaccuuaac cccagucggc     1680

cgccuaauca ccgcaaaccc cgucaucacc gaauccaccg aaaacuccaa aaugaugcug     1740

gaacuagacc cacccuuugg agacuccuac aucguuauag gaguaggcga gaaaaagauc     1800

acccaccacu ggcaccgcuc cggaagcacg aucggcaaag ccuucgaagc caccguccgg     1860

ggagccaaac gcauggcagu acuaggagac acagcauggg acuucggaag cgugggcggg     1920

gcccugaaca gccuaggcaa aggaauccac cagauauucg gcgccgccuu caaaagccua     1980

uucggcggca uguccugguu cucgcaaaua cuuaucggca cacuauuaau guggcucgga     2040

cucaacacaa agaacggcuc aaucagccua augugucucg cucugggagg aguccuaaua     2100

uuccucagca cggcagucag cgcggacguc ggcugcucag ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9507
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-long mRNA Design 3 opt

<400>  9507
gggagaaagc uuaccaugcg cauaaucaac gcccgaaaag agaaaaagcg acgcggggcc       60

gacacgagcg ucgguauagu aggccuacuu cugaccacag ccauggcggc cgaagucacg      120

cgccggggca gcgccuacua cauguaccua gaccgaaacg acgcaggaga agcaaucagc      180

uucccaacca cgcuaggcau gaacaaaugc uacauacaaa ucauggaccu aggucacaug      240

ugcgacgcaa ccaugagcua cgaaugcccu augcuagacg aaggagucga acccgacgac      300

guggacugcu ggugcaacac cacaagcaca ugggucgucu acggaaccug ccaucacaaa      360

aagggcgaag cacgcagaag ccgacgcgcc gucaccuugc caagccacag cacccggaaa      420

cugcaaacaa gaagccaaac guggcuagaa agucgcgaau auaccaaaca ucucauacgc      480

gucgaaaacu ggaucuuccg aaaccccggc uucgcacucg cagcggccgc aauagcaugg      540

cuccuagggu ccagcacaag ccaaaaaguu aucuaccucg uaaugauccu acucaucgcu      600

ccagcauacu ccauacgaug caucggcguc agcaaccgag auuucgucga aggaaugucc      660

ggaggcaccu ggguagacgu cguacucgag cacggcggcu gcgucaccgu cauggcccaa      720

gacaaaccaa cugucgacau agaacucguc accaccaccg ucucaaacau ggccgaagug      780

cgcagcuacu gcuacgaagc gagcaucagc gacauggcca gcgacuccag augucccacu      840

caaggagaag ccuaccucga caaacaaucc gacacccaau acgugugcaa acgcacgcua      900

gucgaccgag gauggggaaa uggaugcgga cucuuuggaa aagguucguu aguaaccugc      960

gccaaauucg caugcuccaa aaagaugaca ggcaaaucaa uacagccgga aaaccucgaa     1020

uacagaauca ugcucagcgu ccacggcagc caacauagcg gaaugaucgu aaacgacacg     1080

ggacacgaaa cagaugaaaa uagggcaaaa gucgagauca ccccuaacag uccccgagcc     1140

gaggcggcac uaggcggauu cggaucacua ggacucgacu gcgagccccg cacuggccug     1200

gacuucuccg accucuacua ccucaccaug aacaacaaac acuggcuagu gcacaaggaa     1260

ugguuccacg acaucccccu acccuggcac gccggcgccg acaccggaac uccccauugg     1320

aacaacaaag aagcacuggu cgaauuuaaa gacgcacacg cgaaacgaca aacagucgua     1380

guacucggau cccaagaagg ugccguccac acagcacuag ccggagcgcu ggaggcagag     1440

auggauggcg ccaagggccg gcucuccucc ggccaccuaa agugucgucu aaaaauggac     1500

aaacuccgcc uaaaaggcgu gucguacagc cuaugcaccg cagccuucac cuucaccaaa     1560

auccccgccg agacacuaca cggaaccguc acagucgaag uccaauacgc cggcacagac     1620

ggcccgugca aagugccggc ccaaauggca guggacaugc agacccuaac accagucgga     1680

cggcuaauca cagccaaucc cguaaucaca gaaagcacag aaaacagcaa aaugauguua     1740

gaacuagauc ccccauucgg cgacagcuac auagucauag gcgucggaga gaagaaaaua     1800

acacaccacu ggcacagaag cggcagcaca aucggaaaag cauuugaagc aaccguccgc     1860

ggagcaaaaa gaauggcagu acugggcgac accgcauggg acuucggauc aguaggcggc     1920

gcacuaaacu cauuaggcaa agggauacac caaauauucg gagcugcauu caaaagccuc     1980

uucgggggca ugagcugguu cucacaaaca cucaucggca cccuacugau guggcuggga     2040

cuaaauacca aaaacggaag caucucgcuc augugccucg ccuuaggagg aguccuaauc     2100

uuccucagca ccgcuguaag cgccgacguc ggcugcagcg ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9508
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-long mRNA Design 3 opt

<400>  9508
gggagaaagc uuaccaugcg cauaaucaau gccaggaaag aaaagaaaag gcgcggcgca       60

gaaaccucag ugggaaucgu cggacuacua cugaccacag caauggccgc cgaggugaca      120

cgacgcggau cugccuacua cauguaccua gaccgaaacg acgcuggcga agcgaucucc      180

uuccccacaa cccucggaau gaauaaaugc uacauacaaa uaauggaccu aggccacaug      240

ugugacgcca caauguccua cgaaugccca augcucgacg aaggugugga acccgacgac      300

guggacugcu ggugcaacac aacgucaacg uggguaguau acggaaccug ccaccacaag      360

aaaggagaag caagaagaag ccgacgagcu guaacacucc cgagccacag cacgcgaaaa      420

cuccaaacca gaagccaaac guggcuagaa agcagagaau acaccaaaca ccuaauacgc      480

gucgagaacu ggauauuucg aaaccccgga uucgcacucg cagccgccgc aauagccugg      540

cuacuaggca guucaaccag ucaaaaaguc aucuaccuag uuaugauacu acucaucgca      600

cuagcauaua gcauccgaug uauagggguc ucaaaccgcg acuucguaga gggaaugagc      660

ggcggcaccu ggguagacgu cguccucgaa cacggcggau gcguuaccgu aauggcgcag      720

gacaaaccca ccguggacau agagcuaguu accacgacag uaagcaacau ggcggaaguc      780

agaucauacu gcuacgaagc aagcaucagc gacauggcaa gcgacagcag augcccaacg      840

caaggagaag ccuaccucga caaacagagc gacacacaau acgucugcaa acgcacgcuu      900

guagaccgag gauggggcaa cggaugcgga cuguucggaa aaggcucauu agucacaugc      960

gccaaauucg cgugcagcaa gaaaaugacc ggaaaaagua uccaacccga aaaccucgaa     1020

uaccgaauca ugcucuccgu ccacggauca cagcacagcg gaaugauagu aaacgacaca     1080

ggacacgaaa ccgaugaaaa ccgggcgaaa guagaaauca cuccaaacuc cccuagagcg     1140

gaagccacac uaggcggcuu cggaagccua ggccucgacu gcgaaccacg cacgggccuc     1200

gacuucagcg accucuacua ccuuaccaug aacaacaaac acuggcuggu acacaaagaa     1260

ugguuccacg acaucccccu accauggcac gcaggagcag acacaggcac accccacugg     1320

aacaauaaag aggcacucgu cgaguucaaa gacgcccacg caaaacgaca aaccgucguc     1380

guccuaggaa gucaagaagg cgcgguccac acggcacucg ccggugcgcu agaagcagag     1440

auggacggcg cgaaaggacg acuaagcucc ggacaucuca aaugcaggcu caaaauggac     1500

aaacuccggc ugaaaggagu aagcuacucg cucugcaccg cugccuucac auucaccaaa     1560

auccccgccg aaacacucca cggcaccguc accgucgagg uacaauacgc aggcacagau     1620

ggaccgugca aagucccagc acaaauggcc guagacaugc aaacacugac acccgucgga     1680

cgacucauca ccgcuaaccc cgucaucacc gaaaguaccg agaacagcaa aaugaugcua     1740

gaacucgacc cgcccuucgg agacucauac aucguaaucg gagucgggga aaagaaaaua     1800

acacaccauu ggcaccgauc cggcuccacu auaggaaaag cguucgaagc cacgguacgc     1860

ggugccaaac gaauggcagu ccucggcgac accgccuggg acuucggaag ugucggaggc     1920

gcauuaaacu cccucggcaa aggaauacac caaaucuucg gcgccgcauu caaaagccua     1980

uucggaggaa uguccugguu cucccaaaua cugauaggga cacuccucau guggcucggu     2040

cuaaacacca aaaacggaag caucucccuc augugccuug cccuuggagg aguacucauc     2100

uucuugucaa cagccguaag ugcugacguc ggcugcagcg ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9509
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-long mRNA Design 3 opt

<400>  9509
gggagaaagc uuaccaugcg aaucauaaac gcuaggaaag agaagaaacg gcgaggagcg       60

gacacauccg ugggaauagu uggccugcuc cuaacaaccg caauggcggc agaaguaacg      120

cgccgaggcu ccgccuacua cauguaccua gaccguaacg augcugggga ggcaauaucc      180

uucccuacca cccucggcau gaacaaaugc uacauccaaa uaauggaccu cggacacaug      240

ugcgacgcca caauguccua cgagugcccg augcuagacg aaggcgucga accagacgac      300

gucgacugcu ggugcaauac aaccagcacc ugggucguau acggcacuug ccaccacaag      360

aaaggagaag ccagacgcag cagacgagcc gucacgcucc caagccacuc cacccgcaaa      420

cuacagaccc gaucccaaac auggcucgaa agccgagaau acacaaaaca cuuaauccgc      480

gucgagaacu ggaucuuccg caaccccggc uucgcacucg cagcggccgc cauagccugg      540

cuccucggga gcagcaccuc ccagaaggua aucuaccugg ugaugauacu ccuaauugca      600

cccgccuacu ccauacggug cauaggcaua uccaaccgag acuucgucga aggcauguca      660

ggaggaaccu gggucgacgu cguucuagaa cacggaggcu gcguaaccgu cauggcccaa      720

gacaaaccaa cagucgacau agagcuaguc accacaaccg ucuccaacau ggccgaaguc      780

agaaguuacu gcuacgaggc aagcaucagc gacauggcca gcgacagccg cugccccaca      840

caaggagaag cauaccucga uaaacaauca gacacccaau acgucugcaa acgcacacug      900

guagacagag gauggggcaa cggcugcggu cucuucggga aaggcucacu gguaaccugc      960

gccaaauucg ccugcuccaa gaaaaugaca gggaaaucca uacaaccaga aaaccuagaa     1020

uaccgcauaa ugcuaucugu ccacggauca caacacuccg gcaugauagu aaacgacacc     1080

gggcacgaaa ccgacgaaaa cagagccaaa guagaaauca cccccaacag cccccgcgcg     1140

gaagcaacac ucggaggauu cggcauacua ggacuggacu gcgaacccag aaccggacug     1200

gauuucagcg accuuuauua ccucacaaug aacaauaaac acuggcuggu ccauaaagaa     1260

ugguuccacg acauacccuu acccuggcau gcgggagcag acacaggcac accgcacugg     1320

aacaauaaag aagcccucgu cgaguucaaa gacgcccacg caaaaaggca aaccgucgua     1380

guccugggcu cccaagaagg agccguacac accgcacucg ccggcgcccu agaagcagag     1440

auggacgggg ccaaaggccg acuaagcucc ggccaccuca aaugccgucu aaaaauggac     1500

aaacuacgcu uaaagggcgu gucauacuca cucugcacag cagccuucac auucaccaaa     1560

auaccggcag aaacacugca cggcacaguc acaguggaag ugcaauaugc cggcacagac     1620

ggcccaugua aagucccagc ccaaauggca guagacaugc aaacgcuaac accagugggc     1680

cgccuaauaa cagccaaccc cgucaucaca gaaaguacag aaaacucaaa aaugaugcua     1740

gagcuagauc ccccauuugg cgacucauac auaguaauag gcgucggcga aaagaaaauc     1800

acccaucacu ggcaccgauc aggaagcaca auuggaaaag ccuucgaagc gacaguaaga     1860

ggagccaaac gaauggcagu acugggagac acagccuggg acuucggaag cgugggcgga     1920

gcacugaacu cgcuaggaaa aggcauccac cagaucuucg gcgcagccuu caagucacuc     1980

uucggcggca ugagcugguu cucccagaua cucaucggca cgcuccucau guggcuagga     2040

cuaaacacga aaaacgguag caucucacug augugccucg cccucggagg aguccucaua     2100

uuccuaucga cagccguaag ugccgacgug ggaugcuccg ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9510
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-long mRNA Design 3 opt

<400>  9510
gggagaaagc uuaccaugcg caucauaaac gccagaaaag aaaagaaaag gcguggcgcc       60

gacacauccg uaggaauagu cggucuacua cucaccacag ccauggcagc cgaagucaca      120

aggagaggcu cagcauacua cauguaccuc gaccgcaacg augccggaga ggccauuuca      180

uucccaacaa cccuaggaau gaacaaaugc uacauccaaa uaauggaccu cggccauaca      240

ugcgacgcca caauguccua cgaaugcccu augcucgacg aaggaguaga accggacgac      300

gucgacugcu ggugcaacac caccucaacc ugggucgugu acggaacaug ccaccacaag      360

aaaggagaag ccagacgcag ccgccgggca gugacccucc ccucccacuc gauccgcaaa      420

cuccaaacac gcagccaaac cugguuagaa ucacgagaau acaccaaaca ucuaaucagg      480

gucgaaaacu ggaucuuccg aaacccaggc uucgcacucg ccgcagccgc aauagcgugg      540

uuacucggaa gcuccacaag ccaaaaaguc aucuaccucg uaaugauacu ucuaauagca      600

ccagccuaca gcauccgcug caucggaguc agcaaccgag acuuugucga gggcaugagc      660

ggcggcaccu ggguggacgu cguacucgaa cacggcggcu guguuacggu cauggcccag      720

gacaagccca cagucgauau agaacucguc acuacaaccg ucagcaacau ggcugaagua      780

cgaagcuacu gcuacgaggc cuccauaagc gacauggcaa gcgacucccg cugccccacc      840

caaggcgaag cauaccuuga uaaacaaucc gacacacaau auguaugcaa acgcacacuc      900

guagacagag gguggggaaa cgggugcgga cucuucggca aaggaucccu cguaaccugc      960

gccaaauucg ccugcuccaa aaagaugacc ggaaagagca uccaacccga aaaccucgaa     1020

uaucggauca ugcucuccgu ccacggaucc caacauagcg gaaugaucgu aaacgacaca     1080

gggcacgaaa ccgacgaaaa ccgcgcaaaa guagaaauca ccccgaacag cccccgcgcg     1140

gaagccaccc ucgggggcuu cggaagccug ggccucgacu gcgaaccccg aacaggacua     1200

gacuucuccg accucuacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccccu accauggcac gcaggcgccg acacgggcac accgcacugg     1320

aacaacaaag aggcauuagu cgaauuuaag gacgcgcacg caaaacgcca gacaguaguc     1380

gugcugggcu cacaagaagg cgccguacac accgcccuug ccggcgcacu agaagcagag     1440

auggacggcg caaaaggcag acuauccucc ggccaccuaa aaugcagacu aaagauggac     1500

aaacuccggc ugaaaggagu guccuacagc cuuugcaccg cugccuucac guucacaaaa     1560

auccccgccg agacccucca cggaacggua acaguagagg uccaguacgc aggcaccgac     1620

ggaccgugca aagucccagc ccaaauggcc gucgacaugc aaacgcucac ccccgucggc     1680

cgacugauaa ccgcgaaccc agucaucacc gaguccaccg aaaacuccaa aaugaugcua     1740

gaacuagacc caccauucgg ggacucauac aucgugauag gaguaggcga aaagaaaauc     1800

acccaccacu ggcaucguuc cggcuccaca aucggcaaag cauucgaagc cacagucaga     1860

ggcgccaaac gaauggccgu gcucggagac accgccuggg acuucggaag cguaggcggc     1920

gcccucaacu cccucggaaa aggaauccac caaaucuucg gggccgccuu caagucccuc     1980

uucggcggaa ugucaugguu cucucagaua cucaucggaa cucugcucau guggcuaggg     2040

cuuaacgcca agaauggcuc gaucucacuc augugccuag cauuaggagg aguccucaua     2100

uuccuaagca ccgcgguauc cgcagacgua gggugcagcg ucgacuucag uaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9511
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-long mRNA Design 3 opt

<400>  9511
gggagaaagc uuaccaugcg aauaauaaac gccagaaaag aaaagaaacg acgaggagcc       60

gacaccucag uaggcaucgu aggacuacua cucacgacag caauggcagc agagguaacc      120

cgccgcggga gugcauacua cauguaccuu gacagaaacg acgcgggaga agcgauaucc      180

uucccaacaa cgcucgggau gaacaaaugc uacauccaaa uaauggaccu cgggcauaug      240

ugcgacgcaa caaugucgua cgaaugccca augcuagacg aaggcguaga accagacgac      300

gucgacugcu ggugcaacac aaccagcacc ugggucguau acggcacgug ucaccacaag      360

aaaggagaag cccaacgcuc caggcgagcc gucacgcucc ccagccacag cacccgaaaa      420

cuccaaaccc gcagccagac auggcucgaa ucaagagaau acaccaaaca ccuaauacga      480

gucgaaaacu ggaucuuccg caaccccgga uucgcccucg cagccgcagc gauagcgugg      540

cuccugggca gcaguaccag ccagaaagua auauaccuug ucaugauacu ccucauagcc      600

cccgccuaca guauacgcug cauaggcgua agcaaccgcg acuucguaga aggaaugagc      660

ggcggaacau gggucgacgu ggucuuagaa cacggcggcu gugugacagu aauggcacaa      720

gacaagccca ccguagacau agaacugguc acaacaacag uaagcaacau ggcagaaguc      780

cgcucguacu gcuacgaggc uucaaucucc gacauggcau cagacucaag gugcccaacu      840

caaggagaag cauaccucga caaacaaucc gacacccaau acgucuguaa acgaacacuu      900

gucgaccgcg gaugggggaa cggaugcggc cucuucggga aaggaucccu cguaaccugc      960

gccaaauucg cgugcucgaa gaaaaugacg ggaaaaagca uacagcccga aaaccuugaa     1020

uacagaauaa ugcucagcgu ccacggauca caacacagcg gcaugauagu caacgacaca     1080

ggccacgaaa ccgaugaaaa ccgggccaaa guagaaauca cgccaaacuc cccacgagcc     1140

gaagcgacac ucggaggcuu cggaucccug gggcuagacu gcgaaccucg aacaggcuug     1200

gacuucagcg accuguacua ccuaacaaug aacaacaaac acuggcuggu acacaaagaa     1260

ugguuccacg acaucccacu ccccuggcac gccggagcag acaccggaac cccucacugg     1320

aacaacaaag aagcacucgu cgaguucaaa gacgcacacg ccaaaagaca aacaguagug     1380

guccucggga gccaagaagg agcaguacac acagcccucg caggcgcccu cgaagcagag     1440

auggacggag caaaaggacg ccugagcuca ggacaccuaa aaugcagacu aaaaauggac     1500

aaacuccgac uaaaaggcgu cucuuacuca cuauguacag cagcguuuac cuucacaaaa     1560

aucccagcgg aaacauuaca cggaaccguc accgucgaag uacaauaugc cggaaccgac     1620

ggcccaugca aaguccccgc acaaauggca gucgacaugc aaacacucac ccccgucgga     1680

cgccugauca cagcgaaccc aguaaucacc gagucaacgg agaacagcaa aaugaugcua     1740

gagcuggacc cgccauuugg agauuccuac auagucaucg gcgucggaga gaagaaaauc     1800

acacaccacu ggcaucgcag cggaagcaca auagguaaag cauucgaagc gacaguccgu     1860

ggagcaaaaa ggauggcggu ccucggcgau accgcauggg acuucggcag cguaggcgga     1920

gcccuaaaca gccucggaaa aggcauacac caaaucuucg gcgccgccuu caaaagccua     1980

uucggcggaa ugagcugguu cagccaaauc cuaauaggca cacuccucau guggcucggc     2040

cucaacacca aaaacggaag caucagccuc augugccuag cccucggcgg cguccuaauc     2100

uuccugucaa cagccgugag cgcagacguc ggcugcagcg uggacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9512
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-long mRNA Design 3 opt

<400>  9512
gggagaaagc uuaccaugcg cauuaucaac gcgcgaaaag agaaaaagag acgaggcgca       60

gacaccuccg uaggcaucgu cggccuacug cucaccaccg ccauggcagc agaagucacc      120

agaagagguu cagcguauua cauguaccuc gaccgaaacg acgcagggga agcaaucagc      180

uucccaacca cccuaggaau gaacaagugc uacauacaaa ucauggaccu gggacacaug      240

ugcgacgcca ccaugucaua cgaaugcccg augcucgacg aaggaguaga acccgaugac      300

gucgacugcu ggugcaacac caccagcacc uggguagucu acggaaccug ccaccacaag      360

aaaggcgaag cacgacgcuc gcgcagagca gucacccucc caucacacuc aacaagaaaa      420

cuacaggcca gaucacaaac auggcucgaa ucccgagagu auaccaaaca ccugauccgc      480

gucgaaaacu ggauauuccg aaaucccggc uucgcacugg ccgccgccgc cauagccugg      540

cugcucggca gcagcaccag ccaaaaagug aucuaccucg ucaugauacu gcucauagcc      600

ccagccuacu caauacgcug cauaggagua ucaaacagag acuucgucga ggggauguca      660

ggaggcaccu ggguagacgu cguccucgaa cacggcggau gcgugacagu cauggcccag      720

gacaaaccca caguagacau cgagcuaguc acaacaacag ucucaaacau ggccgaaguu      780

cgcucauacu guuacgaagc cagcaucagc gacauggcga gcgacucacg cugcccgacc      840

caaggagaag ccuaccugga caaacagucc gacacgcagu acgucugcaa gagaacccua      900

guagacaggg gcuggggcaa cggcugcggg cuauucggga aagguagccu uguaaccugc      960

gccaaauucg ccugcuccaa gaaaaugacc ggcaaaucga uccagcccga aaaucuagaa     1020

uaccgcauaa ugcucuccgu gcacggcagc caacacucag gcaugauugu aaacgacacu     1080

ggccacgaga cggacgaaaa ccgcgcaaaa gucgaaauca caccaaacuc cccacgcgcg     1140

gaggcaaccc ucggaggcuu cggaagccua ggccucgacu gugaaccccg caccggccua     1200

gacuucucag accuuuauua ccucacaaug aacaacaaac acuggcucgu ucacaaagaa     1260

ugguuucacg acaucccgcu ccccuggcac gcaggcgccg acacaggaac accccacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg caaaacgcca aaccgucguc     1380

guacucggcu cgcaagaagg agcaguacac accgccuugg caggcgcccu cgaagccgaa     1440

auggacggcg ccaagggccg gcucagcucc ggccaccuca aaugccgccu aaaaauggac     1500

aaacuacgac uaaaaggagu aagcuacagc cuaugcaccg cugcauucac uuucacaaaa     1560

auacccgcag aaacccuaca cggcaccguc acggucgaag uucaauacgc cggcaccgac     1620

ggacccugca aagucccagc ccaaauggca guagacaugc agacacucac cccagucgga     1680

agacuaauca ccgcaaaccc aguaauaaca gaauccaccg aaaacagcaa aaugaugcuc     1740

gaacucgacc cgccguucgg agacagcuac aucguaaucg gcguaggcga aaagaaaaua     1800

acacaccacu ggcaccgauc cggcuccacc aucggcaagg ccuucgaagc aaccguacga     1860

ggagccaaac gaauggcagu acucggagau acggcuuggg auuucggcag cgucggggga     1920

gcccuaaacu cacuaggaaa aggcauccau caaaucuucg gagcggcuuu uaaaagucua     1980

uucggaggca ugaguugguu cagccagaua cuaaucggca cacuacuaau guggcucggc     2040

cucaacacca aaaacggauc caucucacua augugucucg cccuaggcgg aguacuaaua     2100

uuccucagca ccgccgucag cgccgacgua ggaugcagug uggacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9513
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-long mRNA Design 3 opt

<400>  9513
gggagaaagc uuaccaugcg caucaucaac gcgcgcaagg agaagaaacg ccgaggcgca       60

gacacauccg ucggaaucgu agggcuacuc cucacaaccg caauggccgc agaagugaca      120

cgacgcggau cugcauacua uauguaccua gacagaagcg acgcaggaga agcaauuagc      180

uucccaacga cccucgggau gaacaaaugc uacauacaaa uaauggaccu cggacacaug      240

ugcgacgcaa cgaugucuua cgaaugcccc augcuagacg aaggggugga acccgacgac      300

gucgacugcu ggugcaacac cacaagcacg uggguagucu acggaaccug ccaccacaag      360

aaaggcgaag cccgcaggag caggagagcc guaacacucc ccucccacag cacccgaaaa      420

cugcaaacga gaucacaaac guggcucgaa uccagagaau auacaaaaca ccuaaucaga      480

gucgagaacu ggauauucag aaacccaggu uucgcccuag cagcagcagc aauagccugg      540

uuacuaggaa gcagcaccag ccaaaaagua auauaccuag uaaugauacu ccucauagcc      600

ccagcauaca gcauacggug uaucggagua agcaaccgag acuucgucga aggaaugucg      660

ggaggaacau ggguagacgu aguacucgaa cacggcggcu gcguaacagu cauggcccaa      720

gacaaaccaa caguagacau ugagcuagug acuacaacag ucagcaacau ggcggaaguc      780

cgcucguacu gcuacgaagc aucgaucagc gacauggcaa gcgacaguag augccccaca      840

cagggcgaag ccuacuuaga caaacaauca gacacccaau acguauguaa aaggacccua      900

gucgaucgcg gauggggcaa cggaugcggg cuauuuggca aagguucacu cguaaccugc      960

gcgaaauucg cgugcaguaa gaaaaugaca ggcaaaagua uacaaccaga aaaccucgag     1020

uaccgcauaa ugcuaucggu ccacggcucc caacacagcg gcaugaucgu caacgacacg     1080

ggacacgaaa ccgacgaaaa cagagcaaaa gucgagauca caccaaacuc accacgagca     1140

gaggcaaccc uuggcggauu cggcagucuc ggacucgacu gcgaaccgcg gacaggccuc     1200

gacuucucag accuauauua ccuaaccaug aacaacaaac acuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu accauggcac gccggcgccg acaccggcac accacacugg     1320

aacaacaaag aagcacucgu agaguucaaa gacgcgcacg cgaaacgaca aacaguagua     1380

guacuaggcu cacaagaagg agcaguccac acagcacugg ccggcgcacu agaagccgaa     1440

auggacggcg caaaaggacg ucuaaguagc ggucaccuaa aaugccgacu aaaaauggau     1500

aaacuacgcc uaaaaggggu cucauacucg cucugcaccg cagcauucac cuuuacgaaa     1560

aucccagccg aaacccugca cgggacagua accguagaag uccaauacgc cggcaccgac     1620

ggcccaugca aaguaccagc ccaaauggcc gucgacaugc aaacccugac cccgguggga     1680

agacugauca cagccaaccc agucaucaca gaaagcacgg agaacagcaa aaugaugcug     1740

gaacuugacc cacccuucgg cgacagcuac aucguaauag gggugggaga aaagaaaaua     1800

acacaucacu ggcaccgcuc agguagcaca aucggcaaag cauucgaggc aacagucaga     1860

ggagcaaaac ggauggcagu ccuaggagac acagccuggg auuucggaag cguaggaggg     1920

gcgcucaauu cacuaggcaa gggcauccac caaauauucg gagccgccuu caagucacug     1980

uucggcggaa ugagcugguu cagccaaauc cucaucggca cacuauuaau gugguuagga     2040

cugaacacua aaaacggauc caucagccuc augugccucg cgcugggcgg cguacugaua     2100

uuccugagca cagccguaag cgccgacguc gggugcucag uagacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9514
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-long mRNA Design 3 opt

<400>  9514
gggagaaagc uuaccaugcg cauaauaaac gccagaaaag aaaagaagag aaggggaaca       60

gacacauccg ugggcaucgu cggccuccug cucacaacag ccauggcagc cgaagucacc      120

cgaagaggca gcgcauacua cauguaccua gacaggucag acgcaggcga agcaauaucc      180

uucccaacaa cacuaggaau gaacaaaugc uacauccaga uaauggacuu aggacacaug      240

ugcgacgcaa cgaugucaua cgaaugcccc auguuagacg aaggaguuga accagacgac      300

guggacugcu ggugcaacac uacaucaacg uggguggugu acggaacaug ccaccacaag      360

aaaggagaag cgcgcagauc cagacgagca gugacccuac caagccauag cacccgaaaa      420

cuccaaacaa gaagccaaac cuggcuggag agcagggaau auaccaaaca ccucauaagg      480

gucgagaacu ggauauucag aaacccaggc uucgcacucg ccgcagcagc cauagccugg      540

cuccuaggcu caagcacaag ccaaaaagua auauaucucg ugaugauccu acuaaucgca      600

ccagcauacu caauacgcug caucggcguc aguaacaggg acuucguaga agggaugucg      660

ggaggcacau ggguggacgu uguacucgag cacggaggau gcgucaccgu cauggcacaa      720

gauaaaccca caguggacau cgaacucgua acuaccacag ugucuaacau ggcagaagua      780

cgaucauacu gcuacgaagc gucgauauca gacauggcca gugacucccg gugcccgaca      840

caaggagaag cauaccucga caaacagagc gacacacaau acguauguaa aagaacgcug      900

guagaccgcg gauggggcaa cggcugcgga cucuucggca aaggaucccu cguaaccugc      960

gcaaaauucg cgugcucaaa gaaaaugacc ggaaagagca uacaaccaga aaaccucgag     1020

uaccgcauaa ugcucucagu ccacggaagc cagcacagcg ggaugauagu caacgacaca     1080

ggccacgaaa cagacgaaaa ucgagcaaag guagaaauca caccgaacag cccccgcgcc     1140

gaagccacgc uaggaggauu cggcucccua ggacucgacu gugaaccgcg cacaggacua     1200

gacuucuccg accuauacua ccucacaaug aacaacaaac acuggcuagu gcacaaggaa     1260

ugguuccaug acauaccccu ccccuggcac accggagccg acaccggaac accacacugg     1320

aacaacaaag aagcccuggu agaguucaaa gacgcacacg caaaaagaca aacaguagug     1380

guccuaggca gucaagaagg cgcuguacac acagcacuag caggagcacu agaagccgaa     1440

auggacggcg caaaaggaag acugucguca ggacaccuca agugccgauu aaaaauggac     1500

aaacuaagac ugaaaggagu cucguacagc cucuguaccg cggccuucac auucaccaag     1560

auaccagccg aaacacugca cggaacagua acaguagaag ugcaguacgc cgggacggac     1620

ggaccaugca aagugccagc acaaauggcc guagacaugc aaacacucac gccagucggc     1680

cgccugauca ccgccaaccc cgucauaaca gaagggaccg agaacucgaa aaugaugcug     1740

gaguuggacc cacccuucgg agacagcuac aucguaaucg gagugggcga gaaaaagaua     1800

acgcaccacu ggcaccgcag uggaagcacc aucggcaaag ccuucgaagc caccguccgc     1860

ggcgcaaaga gaauggcagu ccuaggcgac acagcauggg acuucggaag cguaggaggc     1920

guucugaaca gccuaggcaa aggaauccac caaaucuucg gcgcagccuu caaaucacuc     1980

uucgggggca ugucaugguu cagccaaaua cuaauaggca cccuacucau guggcucggg     2040

cuaaacacua aaaacggaag caucagccua augugccucg cccugggagg cguacuaauc     2100

uuccuaagca cagcaguuuc agccgacgug ggaugcagcg ucgacuucuc aaagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9515
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-long mRNA Design 3 opt

<400>  9515
gggagaaagc uuaccaugcg aauaauuaac gcuagaaaag aaaagaaaag acgaggagcc       60

gauacauccg uagggauugu cggacuacuc cuaacaacag cgauggcggc agaagugaca      120

cggcguggca gcgccuacua cauguaccug gaccgaaacg acgcaggcga agccaucuca      180

uucccaacaa cacuuggcau gaauaaaugc uacauccaaa uaauggaucu aggccacaug      240

ugcgacgcga caaugagcua cgaaugccca augcuagacg aaggcgucga acccgacgac      300

guggacugcu ggugcaacac caccuccacc ugggucgugu acgggaccug ccaccacaag      360

aaaggagaag cccgacgcuc aagacgcgcg guaacacuac caucacacuc aacccgaaaa      420

cugcaaaccc gcagucagac cuggcucgag ucgcgagaau acacaaaaca ccugauacga      480

guagaaaauu ggaucuuccg aaacccggga uucgcccucg ccgccgccgc aaucgccugg      540

cuccuaggau ccuccacaag ccaaaaaguc aucuaccucg uaaugauccu ccuaaucgca      600

ccagccuaca gcauaagaug cauaggagua agcaauagag acuucgucga aggaauguca      660

ggcggcacau ggguagacgu cguacuagag cacggaggau gcgucaccgc aauggcccaa      720

gacaaaccaa caguagacau cgaauuaguc acaacaacag uguccaacau ggcagaagua      780

cgcucauacu gcuacgaagc cagcaucagc gacauggcga gcgacucccg gugccccacg      840

caaggcgaag ccuaucucga caaacaguca gacacccaau acgucugcaa acgaacuuua      900

guugaccgcg gcuggggcaa cggaugcggc cucuucggca aaggaucacu cgucacgugc      960

gcgaaauucg ccugcagcaa gaaaaugaca gggaaaucaa uucagccuga aaaccuagaa     1020

uaccgcauca uguuaagcgu ccacggauca cagcacuccg gcaugcucgu gaaugauaca     1080

ggccacgaaa ccgacgaaaa uagagcgaaa gucgaaauca ccccgaacuc uccacgggca     1140

gaagcaacac uaggcggcuu cggcucccuc ggacucgauu gcgagccccg caccggacuc     1200

gacuuuagug accucuacua ccucacaaug aacaacaaac acuggcucgc acacaaagaa     1260

ugguuccacg acaucccgcu acccuggcac gccggagcag ccaccggcac accccauugg     1320

aauaacaaag aagcacucgu cgaguucaaa gacgcacacg cgaaacgcca aacagucgua     1380

gucuuaggaa gucaggaagg cgccguccac accgcucucg ccggcgcccu cgaagccgaa     1440

auggacggcg ccaaaggccg acucuccagc gggcaccuaa aaugccgacu caaaauggac     1500

aaacucagac ucaaaggcgu aucauacagc cucugcacag cagcauucac cuucacaaaa     1560

auccccgcug aaaccguuga cggcacagua accguagaag gccaauacgg gggcaccgac     1620

ggccccugca aaguccccgc gcaaauggcg guagacaugc aaacccucac gcccguaggc     1680

cgccucauaa cagccaaccc cgugaucaca gaaucaacgg aaaacagcaa aaugaugcua     1740

gaacuggauc ccccauucgg agacucauac auagugaucg gugucggaga aaagaaaauc     1800

acccaccacu ggcaccgcuc aggcucaaca auuggaaaag ccuucgaagc aacagucaga     1860

ggcgcaaaac gcauggccgu ccugggagac acagccuggg acuucggcuc agugggcggc     1920

gcccucaacu cccucgggaa aggcauccac cagaucaucg gagcggccuu caaaagccua     1980

uucggcggaa ugucaugguu cagccaaaua cuaauaggaa cacuacuaau guggcuagga     2040

cucaacacca aaaacggcag uaucucacuc augugccuag cccucggcgg agucuuaauc     2100

uuccucucaa cagcuguauc aggcggggua ggcugcuccg uagacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9516
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-long mRNA Design 3 opt

<400>  9516
gggagaaagc uuaccaugcg aaucauaaac gcacgcaaag aaaagaaacg acgagggacg       60

gacaccuccg ucggaaucgu aggccuccuu cugaccaccg caauggccgc cgaagucacc      120

cgccgaggcu cugcauacua cauguaccuc gaccgcagcg augccggaga agcgaucuca      180

uuccccacaa cacucggcau gaacaaaugc uacauucaaa ucauggaccu cggacacaug      240

ugcgacgcca caauguccua cgaaugcccc augcuagaug aaggcgucga accagacgau      300

gucgacugcu ggugcaauac aaccagcacc ugggucgugu acggcacaug ccaccacaag      360

aaaggggaag ccagacgcag ccgacgagca gucacacuac cgucacacag cacacguaaa      420

cuccaaaccc gcagccaaac auggcuggag uccagagagu acaccaaaca ccugauaagg      480

guagaaaacu ggauauuccg caacccuggc uucgcccucg ccgccgcagc aaucgccugg      540

cuacucggcu caucgaccuc ccaaaaagua aucuaccucg ucaugauacu ucucaucgcc      600

cccgcauacu ccauccgaug cauaggagug uccaaccgcg acuucgugga aggcaugucc      660

ggcggaaccu gggucgacgu aguacuagaa cacggaggau gcguaacggu cauggcacaa      720

gacaaaccca caguugauau agagcucgua accaccaccg ucagcaacau ggccgaagua      780

cgcucauacu gcuacgaagc cuccaucucg gacauggcua gcgauucccg cugccccaca      840

caaggagaag cguaccugga caaacaaucg gacacacaau acguaugcaa acggacccua      900

gucgaccgcg guuggggcaa cggcugcgga cuuuucggaa aaggaagccu cguaacaugc      960

gccaaauucg caugcucgaa aaagaugacc ggcaaaagca uacaaccaga gaaccucgag     1020

uacagaauaa ugcucucagu ccacggcucc cagcacuccg gcaugauugu gaaugacacg     1080

ggccacgaaa ccgacgagaa ucgcgcuaag gucgaaauca cacccaauuc accacgcgcg     1140

gaggccacac ucggcggguu uggaagccuc ggccucgacu gcgaacccag aacaggacua     1200

gacuucagcg accuguacua ccucaccaug aacaauaaac acuggcuagu ccacaaagaa     1260

ugguuccacg acauccccuu accauggcau gcaggcgccg acaccggcac accccacugg     1320

aacaacaaag aagcccucgu agaguucaaa gacgcacacg ccaaacgaca aacaguagug     1380

guacugggau cucaggaagg agccguacac acagcccuag caggcgcauu agaagccgaa     1440

auggacggag ccaaaggucg ccucucuagc ggacaucuca agugccgccu gaaaauggac     1500

aaacucagac uaaagggagu cucuuacuca uuaugcaccg cagccuucac auuuuccaag     1560

aucccagccg aaacacugca cggcaccguc accgaggaag uacaguacgc cggaaccgac     1620

ggcccaugca agguaccagc ccaaauggcg gucgacaugc aaacacuaac ccccgucggc     1680

cgacucauua cagcuaaccc cguuaucaca gaaucaaccg aaaacucgaa aaugaugcua     1740

gaacuagacc cacccuucgg agacuccuac aucgucaucg gcguaggcga aaagaaaauu     1800

acacaccacu ggcaccgcag uggcagcacg auaggaaaag cauucgaagc aacuguccgc     1860

ggagccaaac gcauggcagu ccucggggac accgcauggg acuucggaag cgucggaggc     1920

gcccucaacu cgcucggaaa aggaauccac caaauuuucg gagcagcguu caagucccug     1980

uucgggggca ugagcugguu cagccaaaua cucauaggaa cacuacucau gugguuaggc     2040

uuaaacacua agaauggcag caucagccuc augugccucg cccuaggcgg aguacucauc     2100

uuccucagca cagccgugag cgccgacgua ggcugcagcg ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9517
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694532.2 prME-long mRNA Design 3 opt

<400>  9517
gggagaaagc uuaccaugcg cauaaucaac gcccggaaag aaaagaaacg acgaggcacu       60

gacaccuccg ucggaauagu cggacuacua cuaacuacag ccauggccgc agaagucaca      120

aggcgcggau cagcauacua cauguaccua gaccgcucag acgccggcga agccaucagc      180

uucccaacca cacuaggaau gaacaaaugc uacauacaga uaauggaccu gggacacaug      240

ugcgacgcca caaugagcua cgaaugccca augcuagacg aaggcguuga acccgacgac      300

guagacugcu gguguaacac gaccagcacc ugggucguau acggaacaug ccaccacaag      360

aaaggcgaag cuaggcgcuc ccgacgcgcc gucacccuac cgagccauuc caccagaaaa      420

cuccaaacga ggucccagac cuggcuggaa ucccgagaau acacaaaaca ccuuauacga      480

guagaaaacu ggauauuccg aaacccgggg uucgcccugg ccgccgcugc cauagccugg      540

cuccucggcu ccuccacaag ccaaaaagua auauaccugg uaaugauccu acuaauagcc      600

cccgccuaca gcaucaggug caucggcguc ucaaacagag acuucgucga aggcaugucc      660

ggcggaaccu ggguggacgu aguccuggag cauggcggau gcgugacagu aauggcccaa      720

gacaagccaa ccguagacau cgaacuggua acuacaaccg ucagcaacau ggccgaaguc      780

cgaagcuacu gcuacgaagc cagcauaagc gacauggccu ccgacucccg gugcccaaca      840

caaggcgaag ccuaccuaga caaacaaucc gacacgcaau acguaugcaa acgaacccua      900

gucgacagag gauggggcaa cggcugcggc cucuucggca aaggaucacu cgucacgugc      960

gccaaguucg ccugcaguaa gaaaaugaca ggcaaaucga uacaacccga aaaccuagaa     1020

uaccgcauca ugcuaagcgu acacggcagc caacacucag gaaugaucgu gaacgacaca     1080

ggccacgaaa cagacgaaaa ucgagcaaaa guagaaauca ccccuaacag cccacgugcc     1140

gaagcaacac uaggaggauu cggcagccua ggccuugacu gugagccacg aaccggacuc     1200

gacuucuccg accuguacua ccucacgaug aauaacaaac auugguuagu ccacaaagaa     1260

ugguuccacg acauaccauu gccauggcac gcaggcgcgg auaccggaac gccacacugg     1320

aacaacaaag aagcacucgu cgaguucaaa gaugcacacg caaaaagaca gaccguaguc     1380

guacuuggca gucaagaagg cgccguccac accgcgcuug ccggagcccu agaagccgaa     1440

auggacggcg ccaaaggccg acucuccucc ggacaccuca aaugccgacu gaaaauggac     1500

aaacuccgac ucaaaggcgu aagcuacagc uugugcaccg ccgccuucac auucacaaaa     1560

auaccagcag aaacucucca cggcaccgua accguagaag uacaguacgc cgggacagac     1620

ggccccugca agguaccagc acaaauggcc gucgacaugc aaacccuaac acccguuggc     1680

agacuaauca cagcgaaccc aguaaucacc gaaagcaccg aaaauagcaa gaugaugcuc     1740

gaacucgauc ccccauucgg cgacagcuac auagucauag gagucggcga gaagaaaauc     1800

acccaccacu ggcaccgaag cggcagcacc auaggaaaag ccuucgaagc cacagugaga     1860

ggcgcgaaaa gaauggcagu ccucggcgac acagcguggg acuucggcag cgucggcggc     1920

gcacucaacu cacucggcaa aggcauccac caaaucuucg gcgcagccuu caaaagccuc     1980

uucggcggca uguccugguu cagccaaaua cuaaucggga cccuacugau guggcuagga     2040

cuaaacacca aaaacggauc gaucucccuc augugcuuag cacucggcgg cguccuaaua     2100

uucuuaagca ccgccguauc cgcagacgug ggcugcagug ucgacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9518
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-long mRNA Design 3 opt

<400>  9518
gggagaaagc uuaccaugcg aauaaucaac gcacgcaaag agaaaaagag aagaggaaca       60

gacacaucag ucggcaucgu cggauuacug cucacgacgg caauggcggu agaggugacc      120

cgcaggggaa acgccuacua cauguaccua gacagaagcg acgcaggaga ggcuaucucc      180

uucccgacca caaugggcau gaacaaaugc uacauccaaa ucauggaccu agggcauaug      240

ugcgacgcaa ccaugagcua cgaaugccca auguuagacg agggagucga accagacgac      300

guugacuguu ggugcaauac cacaagcacc ugggucguau acggcacaug ccaccacaag      360

aaaggggaag cgaggcgcag ucgacgcgcc gucacccuac ccagccauuc cacacgcaag      420

uuacaaacgc gcagccaaac guggcuagaa agccgagaau acaccaaaca cuuaaucaga      480

gucgaaaacu ggaucuucag aaacccggga uucgcacuag cagccgccgc gaucgccugg      540

cuacuagguu cgagcacaag ccaaaaaguc aucuaccuag ugaugauacu ccuaauagcc      600

cccgcauaca gcauacggug uauaggcgua ucaaacagag auuucgucga gggaaugucc      660

ggcggaacau ggguagacgu aguacuggaa cacggcggau gcguaacagu cauggcacag      720

gacaaaccca cagucgacau agaacucguc accaccaccg ucagcaacau ggccgaagua      780

cgcagcuacu gcuacgaggc aucaauaucc gacauggcau ccgacagcag augcccaacg      840

cagggagagg ccuaccuaga caaacaaucc gacacacagu acgugugcaa aagaacccua      900

guagaccgag guuggggcaa cggcugcgga cuauucggga aaggcucgcu aguaaccugc      960

gccaaauucg ccugcagcaa gaaaaugacc ggcaaaucaa uucaaccaga aaaccucgaa     1020

uaccgaauca ugcucagcgu ccacggaagc caacacagcg gaaugauagu aaacgacacc     1080

ggccacgaaa cagacgagaa cagagcgaaa gucgaaauaa cacccaacag uccccgcgca     1140

gaagccaccc ugggaggauu cggcagccuc ggccuagacu gcgaaccacg aacaggacua     1200

gacuucagcg accuguacua ccugacaaug aacaacaaac auuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu cccuuggcac gcuggcgccg acacaggaac accccacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gaugcacacg caaaacgcca aaccguagug     1380

guccugggcu cacaggaagg agccgugcac acagccuuag cgggcgcgcu cgaagccgaa     1440

auggacggag ccaaaggcag auuaagcagc ggacaccuca aaugccggcu gaaaauggac     1500

aaacugcgac ucaaaggagu auccuacagc cucuguacag cggccuuuac auucacaaaa     1560

auaccagcgg aaacgcuuca cgggacagua acaguugaag uacaauacgc uggaacagac     1620

ggacccugca aaguaccggc acagauggcc gucgacaugc aaacccuaac cccaguagga     1680

cgccugauaa cggccaaccc agugaucacc gaaagcaccg aaaacucaaa aaugaugcuc     1740

gaacucgacc cgccauucgg cgacagcuac auagucauag gagugggaga aaagaaaaua     1800

acgcaccacu ggcaccgcag cggcaguaca aucggaaaag ccuucgaagc aaccgugcgc     1860

ggcgcaaaaa gaauggccgu acuaggagac accgcuuggg acuucggcag cguaggcgga     1920

gcccucaaca gccucgggaa ggggauccac caaaucuucg gagcagccuu caagagccuu     1980

uucggaggca uguccugguu cagccagaua cuaauaggga cccuacuggu auggcuaggu     2040

cucaacacca aaaacggcag cauaagucuu augugccucg cacuaggcgg aguacuaaua     2100

uuccuaagca cagcagugag ugccgacgua ggcugcagcg uagacuucuc caagugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9519
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-long mRNA Design 3 opt

<400>  9519
gggagaaagc uuaccaugcg aaucauaaac gcccgcaaag aaaagaaaag gagaggcaca       60

gacacaagcg uaggaaucgu ggggcuccug cuaacaacag ccauggcggc cgaggucacc      120

cguagaggca gcgcguacua cauguaccua gaccgcagcg acgccggaga ggccaucagc      180

uucccgacaa cccuaggcau gaacaaaugc uacauacaaa uaauggaccu cggccacaug      240

ugcgacgcga ccaugucaua cgaaugccca augcuggacg aaggagucga acccgacgac      300

guagacugcu ggugcaacac aacgagcacg uggguagucu acggaaccug ccaccacaag      360

aaaggcgaag cacgacgauc ccgacgagcg gucacgcucc caagccacuc cacccgaaaa      420

cuccagacca gaagccaaac cuggcuagaa uccagggaau acaccaaaca ccucauccgc      480

guagaaaacu ggauauuccg aaaccccggc uucgcacucg cagcagcagc cauagcaugg      540

cuacucggca gcagcacaag ccaaaaagua auauaccugg ugaugauacu acugaucgcc      600

cccgccuaca gcauccgcug cauuggaguc aguaacagag acuucguaga gggcaugagu      660

ggaggaacau ggguggacgu cguccuggaa cacggaggau gcgucacagu aauggcacaa      720

gacaaaccca cagucgacau cgaacucguu acaacgaccg ucucaaacau ggccgaagua      780

cgcagcuacu gcuacgaggc aaguauaagc gacauggcaa gcgacagccg augcccgacc      840

caaggagaag cauaccugga uaaacaaagc gacacgcaau acgucugcaa aagaacccua      900

gucgaccgcg gaugggggaa cggaugcgga cuauucggaa aagggagccu cguaacaugc      960

gcaaaauucg cgugcucaaa gaaaaugaca ggcaaaagca uccaaccaga aaaccuagaa     1020

uaccgaauca ugcuaagcgu acacggaucc caacacagcg gaaugauagu caaugacacg     1080

ggacaugaaa cagacgaaaa cagagccaaa gucgaaauaa caccaaacag uccccgcgcg     1140

gaagccaccc ucgggggauu cggaagccuc ggcuuagacu gcgagccgcg cacaggacua     1200

gacuucagcg accuauacua cuuaaccaug aauaacaaac auuggcucgu ccacaaagag     1260

ugguuccacg acaucccacu uccguggcac gccggcgcgg acacaggcac accgcacugg     1320

aacaacaaag aagcccucgu ggaguucaaa gacgcccacg ccaaacgaca aacagucgua     1380

guucuagggu cccaagaagg cgcaguacac accgcacucg caggcgcccu cgaggccgaa     1440

auggacggcg ccaaaggccg acuguccagc ggacaccuca agugcagacu aaaaauggac     1500

aagcuccgcc uaaaaggcgu aucauacucg cuauguacag cggcauucac auucaccaaa     1560

aucccagccg aaacccucca cggaaccgua acaguagaag uacaauacgc aggaacagac     1620

ggcccgugca aaguaccggc ccaaauggca guagacaugc aaacacucac acccgucgga     1680

aggcuaauca cagcaaaccc agucauaacg gaaagcacag aaaacagcaa aaugaugcuc     1740

gaacuagacc caccguucgg cgacagcuac auaguuauag ggguaggaga aaagaaaaua     1800

acacaccauu ggcauagauc cggcagcaca aucgggaaag cauucgaagc gacagucaga     1860

ggagcuaaac gaauggccgu acucggcgac acagccuggg acuucggauc cgugggcggg     1920

gcccugaaca gucucggcaa aggaauccac caaaucuucg gagccgcauu caaaucgcua     1980

uucggcggca uguccugguu cucccagauc cuaaucggaa cccuauuaau guggcucggg     2040

cuaaacacca aaaacggauc cauuucccug guaugccucg cccucggagg aguacucaua     2100

uuccugagca cagccgucag cgccgaugua ggaugcuccg uggacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9520
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-long mRNA Design 3 opt

<400>  9520
gggagaaagc uuaccaugcg cauaaucaac gcaagaaaag aaaagaaacg ccgaggcaca       60

gacaccagcg ugggcauagu cggacuacuc cuaacaaccg ccauggccgu ugaagucaca      120

cgacgaggaa gcgcauacua cauguaccua gaccguagcg acgccggaga agcaauaucc      180

uucccaacca cccuaggaau gaacaaaugc uacauccaaa uaauggaccu aggccauaug      240

ugcgacgcua cgaugaguua cgaaugcccc augcuggacg aaggcguaga gccggacgac      300

gucgacugcu ggugcaacac caccagcacc uggguagucu acggaaccug ccaccacaag      360

aaaggcgagg cacgcagaag ccgccgagca gucacccuac caucccacuc aacacgcaaa      420

cuacaaacaa gaucccaaac cuggcuggaa agccgcgaau acacaaaaca ccugauacga      480

gucgagaacu ggaucuuccg aaacccaggc uucgcccucg ccgcagccgc caucgcgugg      540

cuacucggca gcuccacuag ccaaaaagug auauacuuag uaaugauacu ccucaucgcc      600

ccagccuacu cgauccgcug caucggcguc agcaauaggg auuuuguaga aggcaugagc      660

ggcgguacuu gggucgacgu cgugcuagag cacggcggcu gcgucacagu aauggcacaa      720

gacaaacccg ccgucgacau cgaacucgua acgacgacag uaucaaacau ggccgaaguc      780

agaucguauu gcuacgaagc cuccaucagc gacauggcca gcgacucccg cugcccaaca      840

cagggagaag cauacuuaga caaacaaagc gacacacaau acguaugcaa acgaacccua      900

gucgaccgcg gaugggggaa cgggugcggu cuauucggaa aaggaucacu cguaaccugc      960

gccaaauucg cgugcagcaa gaaaaugacc ggcaaaucga uacaaccaga aaaccucgaa     1020

uacagaauca ugcuauccgu ccacggcucc caacacucug gcaugaucgu aaacgauacc     1080

ggccacgaaa ccgacgaaaa ccgagccaaa guggaaauca ccccgaacag cccucgggca     1140

gaagccacgc ugggcggguu cgggagccua ggacucgacu gugaaccacg caccggacua     1200

gauuucagcg accuauacua ccugaccaug aacaacaaac acugguuagu ccacaaagaa     1260

ugguuccacg acauaccacu cccauggcac gcaggcgccg acacaggcac cccacacugg     1320

aacaacaaag aagcacuggu agaguucaaa gacgcccacg ccaaacgcca aacggucgua     1380

gugcuagggu cacaggaagg cgcgguccac accgcccucg ccggcgcccu ggaagcagaa     1440

auggacggug ccaaaggacg ccucagcuca ggacaccuaa aaugccgacu caaaauggac     1500

aaacugcgcc ucaaaggggu cucauacuca cuaugcaccg ccgccuucac cuucaccaaa     1560

aucccagccg aaacacucca cggaacagua accguggaag uccaguacgc cggcaccgac     1620

ggacccugca aggugccagc acaaauggcc guagacaugc aaacccucac cccagucgga     1680

cgccuaauaa cugccaaccc cgucaucacc gaaagcacag aaaacucaaa aaugaugcuu     1740

gaacuggauc ccccauuugg cgacagcuac aucgucaucg ggguuggcga aaagaaaauc     1800

acacaccacu ggcaucgcuc aggcuccacc aucggcaaag cguucgaagc caccguccgc     1860

ggcgcaaaac gcauggccgu acugggagac accgcauggg acuuuggaag ugucggaggc     1920

gcccugaacu cacuagggaa aggaauacac caaaucuucg gagcagcguu caaaagccuc     1980

uucggcggaa ugaguugguu cagccaaauc cuuauaggaa cccuccucgu auggcucggg     2040

uuaaacacca aaaacgggag cauuagccuu acaugccuag cccucggcgg cguacucaua     2100

uuccucagca ccgcugucuc ggccgacguc ggcuguuccg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9521
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-long mRNA Design 3 opt

<400>  9521
gggagaaagc uuaccaugcg gaucaucaac gccagaaaag aaaagaaacg gcgcggagcg       60

gacacaucag ucgggaucgu aggccuacuu cucaccacgg caauggcagu cgaaguaaca      120

agacgcggaa gcgccuacua uauguaccuc gaccgcuccg acgccggaga agcaaucagc      180

uuccccacua cccuaggcau gaacaaaugc uacauccaaa ucauggaccu cggacacaug      240

ugugacgcca cgaugucaua cgaaugcccc augcucgacg aaggagugga acccgacgac      300

guagacugcu ggugcaacac cacaucaaca uggguugucu acggaaccug ccaccacaag      360

aaaggagaag cccgucgaag ccggcgagcc guaacacucc cuucgcacuc aacccgaaaa      420

cugcaaacaa gaucccaaac auggcuagaa agucgugaau acacaaaaca ucuaauacgu      480

guggagaacu ggaucuuccg aaaccccgga uucgcauuag cagccgccgu cauagccugg      540

cugcuaggaa gcagcaccag ccaaaaagua auauaccugg ucaugauccu acucauagcg      600

ccagccuacu ccaucagaug uauaggcguc ucaaaccggg acuucgucga gggaaugucc      660

ggaggcacuu gggucgacgu gguacuggaa cacggcggau gcgucacagu cauggcacaa      720

gacaaaccaa cuguagacau agaacucguc acgaccacgg ucagcaacau ggccgagguc      780

agaagcuacu gcuacgaagc cagcauuuca gacauggcaa gcgacagccg gugcccgacc      840

caaggagaag cguaccuaga caagcaaagc gacacccaau acgucugcaa aagaacccuc      900

gucgaccggg gcuggggcaa cggcugcgga cucuucggca aaggaagucu aguaacuugc      960

gcaaaauuug cgugcucgaa gaaaaugacc ggcaaaucaa uccaacccga gaaccuagag     1020

uacagaauaa ugcuaucagu ucacggcagu cagcacuccg gaaugaucgu aaacgacacc     1080

ggccacgaaa ccgacgaaaa ccgcgcaaaa gucgaaauaa cacccaacag cccccgagcc     1140

gaagccacac ucggaggguu cggcucccua ggacuagacu gcgagccacg caccggacuc     1200

gacuucucag accuauacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccgcu ccccuggcac gccggagcgg acaccggcac accccacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacaug caaaacgcca aaccguaguc     1380

guacucggca gccaggaagg ggccgugcac acagcccuag caggagcccu cgaagccgaa     1440

auggacggag ccaaaggacg acucucaagc ggccaccuca aaugccgacu aaaaauggac     1500

aaacuacgac uaaaaggcgu cagcuauagc cugugcaccg cagccuuuac cuuuacuaag     1560

auucccgcgg aaacccuaca uggcaccguc accguagagg uacaauacgc cggcaccgac     1620

ggcccaugca aaguccccgc ccaaauggcc gucgacaugc aaacgcucac accagucgga     1680

agacuaauaa cagccaaccc cgucaucacc gaaaguaccg aaaacaguaa aaugaugcua     1740

gaacucgauc cacccuucgg agacucauac aucgucaucg gagucggcga aaagaaaaua     1800

acacaccauu ggcauagguc gggcuccaca auaggaaaag cauucgaagc caccguccgc     1860

ggugcaaaac gaauggcagu ccuaggggac acagcauggg acuucggcag cgucggaggu     1920

gcacuaaacu cccucggcaa aggcauccac caaaucuucg gcgcagccuu caaaucccug     1980

uucggaggca uguccugguu cagccaaauc cucaucggca cccuauuagu auggcuaggc     2040

cucaacacga aaaauggcuc gauaucacua acaugucucg cccuaggcgg aguccucauc     2100

uuccuaagca ccgccguauc cgcagacguc ggaugcuccg uagacuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9522
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-long mRNA Design 3 opt

<400>  9522
gggagaaagc uuaccaugcg aauuauaaac gcccgcaaag agaagaaaag acgcggagcc       60

gacacauccg uggguaucgu cggacuccuc cuaaccaccg ccauggccgu cgaaguaacc      120

cgacgcggau ccgcguacua cauguaccuu gaccgcucag acgcggggga agccauaucu      180

uuucccacca cacuaggagu caacaaaugc uacauccaaa ucauggaccu aggccacaug      240

ugcgacgcca caaugagcua cgagugcccc augcucgacg aagggguuga accagacgac      300

guugacugcu ggugcaacac cacguccacc uggguugucu auggaaccug ccaccacaag      360

aaaggcgaag cccgccgguc ccgccgagcc gucacccugc ccagccacag cacacgcaaa      420

cuacaaaccc gaucucaaac auggcuagag ucacgagaau auacaaaaca ccucauccga      480

gucgaaaauu ggaucuuccg caacccaggc uucgcgcucg ccgcagccgc caucgcaugg      540

cuccucggau ccucaaccuc acaaaaaguu auauaccugg uaaugauauu acucauagca      600

ccagccuacu caauacgaug cauaggaguc uccaaccgcg acuucgucga gggcaugagc      660

ggaggcaccu ggguagacgu cguccuagaa cacggaggcu guguuaccgu cauggcgcaa      720

gacaaaccca cagucgacau cgaacucgua acaaccaccg ucuccaauau ggcggaggua      780

cgaucauauu guuacgaagc aucaauauca gacauggcga gcgacagucg cugcccgacc      840

caaggcgaag ccuaucuuga caaacaaagc gacacacagu acguaugcaa acgaacacuc      900

guagaccgag gcuggggcaa cggcuguggc cuguuuggca aagguagccu gguaacaugc      960

gcaaaauucg ccugcuccaa gaaaaugacc ggcaaaucaa uacaacccga aaaccuagaa     1020

uaccgcauca ugcuguccgu ccacggcagc caacacagcg gcaugaucgu caacgacacc     1080

ggacacgaaa ccgacgaaaa ccgcgccaaa guagagauaa caccuaacuc accccgagcc     1140

gaagccacac ucggaggcuu uggcagccua ggacucgacu gcgaaccccg aacaggacuc     1200

gacuucuccg accuauauua ccuaacaaug aacaacaaac acuggcuagu acacaaagaa     1260

ugguuccacg acauaccacu acccuggcac gccggagccg acaccggaac accccacugg     1320

aacaacaaag aagcacucgu cgaguucaag gacgcacacg ccaaacggca gaccgucguu     1380

guccuaggcu cacaagaagg cgcgguccac accgcucucg caggcgcccu cgaagcagaa     1440

auggacggcg ccaaggggag gcucuccucc ggacaccuca agugccgccu aaaaauggac     1500

aaacuacggc ucaaaggagu aagcuacagc cucugcacag cagcauucac auucaccaaa     1560

auaccagcag aaacgcuaca uggaacaguc acggucgaag uacaauacgc gggcacagac     1620

ggacccugca aagugcccgc acaaauggcg guugacaugc aaacccuuac gccagucggc     1680

cgacucauca ccgccaaccc cgucauuaca gaauccacag aaaacuccaa aaugaugcuu     1740

gaacuggacc cacccuucgg cgacucguac auaguaauag gcgucggcga caagaaaauc     1800

acccaccacu gguaccgcuc cggcucaaca aucggaaaag cauucgaagc aaccguccga     1860

ggugccaaac guauggcagu acuaggcgac accgccuggg acuucggcuc cguaggagga     1920

gcacucaacu cccucggaaa aggaauccau caaauauucg gagccgccuu caagucccua     1980

uucggcggca ugucaugguu uagccagauc cucauaggaa cccugcuagu auggcuaggc     2040

cucaacacca aaaacggguc aaucucuuua accugccuag cauuaggggg aguucucaua     2100

uuccucagca cagcaguaag cgccgacgua ggcuguuccg ucgauuucuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9523
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-long mRNA Design 3 opt

<400>  9523
gggagaaagc uuaccaugcg cauaaucaac gcacgcaaag aaaagaaaag acgcggagca       60

gacacaagcg ugggaauugu aggccugcua uuaaccacug ccauggcggu agaaguaaca      120

cgacgcggau cagcauacua cauguaccug gacagaagcg acgcuggcaa agccaucucc      180

uucccaacaa cgcugggagu aaacaaaugc uacauccaaa uaauggaccu agggcacaug      240

ugcgacgcca caaugagcua cgaaugcccg augcucgacg agggaguaga accagacgac      300

gucgacugcu ggugcaacac aacuagcaca uggguaguau acggcaccug ucaccacaag      360

aaaggagaag cacgccggag ccgacgggca gucacgcuac ccucacacag cacacgaaaa      420

cuacaaacuc gcucccaaac cuggcucgaa agccgagaau acaccaaaca ccuaauccgc      480

gucgagaacu ggaucuuccg caacccagga uucgcacuag cagcugcagc aaucgcgugg      540

cugcucggaa gcagcacaag ccaaaaagua auauaccuag uaaugauacu ccucaucgcc      600

cccgccuaua guauacggug caucggcguc agcaaccgag acuucgucga aggcaugagc      660

ggcggcacgu gggucgacgu cguccucgaa cauggcggau gcgucacagu aauggcccag      720

gacaaaccaa ccgucgacau cgaacuuguc acaacgaccg ugagcaacau ggccgaaguc      780

cgcuccuacu gcuaugaagc cuccaucuca gauauggccu cagacagccg augcccaacc      840

cagggcgagg cauacuugga caaacaaagc gacacccaau acgucugcaa gcgaacacug      900

gucgaccgag ggugggguaa uggaugcggc cuauucggca agggaagccu agucaccugu      960

gcaaaguucg ccugcucaaa gaaaaugaca gggaaaucca uccaacccga aaaccucgag     1020

uaccgcauaa uguugagcgu ccacggcucc caacacagcg gcaugauagu aaacgacauc     1080

ggccaugaaa ccgacgaaaa ucgagcaaaa guagaaauaa caccuaacag uccgagagcg     1140

gaagcaacac uaggaggcuu cggcucgcua ggccuagacu gcgaaccccg aaccggacuc     1200

gacuucagcg accuauacua ucucacuaug aacaacaaac acuggcucgu gcacaaagag     1260

ugguuccacg acaucccgcu ccccuggcac gcaggagccg acacaggcac cccacacugg     1320

aacaacaaag aagcgcuagu cgaguucaaa gacgcacacg cuaaaagaca aaccgugguc     1380

guccuaggcu cacaagaagg cgcaguacac acagcacuag ccggcgcucu cgaagcagaa     1440

auggacggag ccaaaggucg acuauccucc ggccaccuaa aaugccgccu caaaauggau     1500

aaacuccgcc ucaaaggcgu aucauacagu cuaugcaccg ccgcauucac auucacaaaa     1560

auaccagccg aaacccucca cggcaccgua accguagaag uacaguacgc aggaaccgac     1620

ggaccaugca aagugccagc ccaaauggca gucgacaugc aaacacucac uccagucgga     1680

cgacuaauca ccgcuaaccc cgucauaacc gagagcacgg aaaauuccaa gaugaugcua     1740

gaacuggauc ccccguucgg cgacagcuac auagucaucg gagucggaga caagaaaauc     1800

acacaccacu ggcaccgaag cggcagcacc aucggaaaag cguucgaagc aaccgucaga     1860

ggcgccaaac gaauggccgu acucggagac acggccuggg acuucggcuc cgucggcgga     1920

gcacucaauu cccucggaaa agguauccac caaauuuucg gcgccgcauu caaaucacua     1980

uuugggggca uguccugguu cucgcagaua cuuaucggca cccuccuagu auggcuagga     2040

cuaaacacca aaaacggcag cauaagccuc acaugccuag cccucggagg gguccuaaua     2100

uuccugagca cagccguaag cgcugacguc ggcugcagcg ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9524
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-long mRNA Design 3 opt

<400>  9524
gggagaaagc uuaccaugag aaucaucaac gcaagaaaag aaagaaaaag aagaggcgcc       60

gacacaucaa ucggcaucgu cggccugcua cuaacaacag cuauggcagc agaaaucacc      120

cgacgaggcu cagcguacua cauguaccuc gacagaagcg acgccggaaa agcuaucagc      180

uucgccacga cacugggagu caacaagugc cacgugcaga uaauggaucu cggacacaug      240

ugcgacgcaa ccaugaguua ugagugcccc augcuagacg aaggcguaga gccagacgac      300

guagacugcu ggugcaacac aacaagcacc uggguaguau acggcacaug ccaccacaag      360

aaaggagaag caagacgaag caggcgcgca guaacacuac ccucacacag cacacgaaaa      420

cuccaaacca gaagccagac auggcucgag agccgugaau acacaaaaca ccuaauaaaa      480

guggaaaacu ggauauuccg caacccaggg uucacccuag uagccguagc aaucgcgugg      540

cuucucggga gcagcaccuc ccagaaaguc auauaccucg uaaugauccu ucucauagcu      600

ccggccuaca gcauacgaug cauaggcgua agcaaccgcg acuucgucga aggaauguca      660

gggggaacau ggguggacgu aguacuagaa cacggaggcu gcgucacggu aauggcacaa      720

gacaaaccaa ccgucgauau cgagcuaguc accaccacag ucagcaacau ggcggaagua      780

cgaagcuacu gcuacgaagc cucaaucagc gacauggcga gcgacucucg cugcccaaca      840

caaggcgaag cauaccucga uaaacaaagc gacacgcagu acgucugcaa acgcacccuc      900

gucgacagag gguggggcaa cggguguggc cuauuuggaa agggaucauu agucacaugc      960

gcaaaauuca caugcagcaa gaaaaugaca gggaaaagca uccaaccuga gaaccucgaa     1020

uaccgcauaa ugcucagcgu ccauggauca caacacuccg gcaugaucgu caacgacacg     1080

ggacacgaaa cagaugaaaa ucgagcgaaa guagagguca caccgaauag ccccagagcc     1140

gaagccacac uaggaggauu cggaagccuc ggccuggacu gcgaaccgcg aaccggccuc     1200

gacuucagcg accuauacua ccucaccaug aacaacaaac auuggcuagu ccacaaagaa     1260

ugguuccacg acaucccccu ccccuggcac gcaggcgccg acacgggaac cccucacugg     1320

aacaacaaag aagcacuggu ugaguucaaa gacgcacacg ccaaaagaca aacaguagua     1380

guauuaggga gccaggaagg agccguacac acagcccucg caggcgcccu agaagccgaa     1440

auggacggag ccaaaggcag acuguucagu gggcaccuca aaugcaggcu aaaaauggac     1500

aaacuacgcc uaaaaggcgu aagcuacagc cuaugcacag ccgcauucac cuucacaaag     1560

guccccgcgg aaacacugca ugggacggua acaguagaag ugcaauacgc aggcacagau     1620

ggacccugca aaguaccagc acaaauggcc guagacaugc aaacacuaac acccgucgga     1680

agacucauca ccgcaaaccc agucaucacg gaaucaacag aaaacagcaa aaugauguua     1740

gaacuggacc cacccuucgg cgacucauac auaguaauag gcgucggaga caagaaaauc     1800

acgcaucacu ggcaccgcag cggcagcaca auuggcaagg cauucgaagc aacagucaga     1860

ggagcgaaac gaauggcagu acucggcgau acagccuggg acuuuggauc cgugggaggc     1920

guguucaaca gccucggaaa agguauccac caaaucuucg gcgcagcguu caaaucacua     1980

uucggcggca ugagcugguu cagccaaaua cucaucggaa cacuccuagu auggcuagga     2040

cucaacacca aaaacggcag caucagccug acaugccuag cgcucggagg agucaugaua     2100

uuccucucaa ccgcaguguc ggccgacgua gggugcagug ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9525
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-long mRNA Design 3 opt

<400>  9525
gggagaaagc uuaccaugag aauaaucaac gcccgcaaag agcgcaaaag aaggggagca       60

gacacgagca uaggcaucau cggacuccug cucaccaccg caauggcagc agaaaucacc      120

agaagaggau cagccuacua cauguaccug gacagaagcg acgcgggaaa agcaauaucc      180

uucgcaacga aucuaggcgu caacaaaugc cacguacaga ucauggaccu aggucauaug      240

ugcgacgcca ccaugucaua cgaaugccca augcuagacg aaggcgucga accagacgac      300

gucgacugcu ggugcaacac cacguccacc ugggugguau acggcacaug ccaccacaag      360

aaaggcgaag cccgaaggag cagacgcgcu gucacccuac caagccacag uacgcgcaag      420

cuccaaacaa gaagccagac cuggcuagaa agccgcgaau acacaaagca ccuaaucaaa      480

gucgaaaacu ggaucuuccg aaacccagga uucgcccucg cagccgucgc aaucgcaugg      540

cugcuaggaa gcuccaccag ccaaaaggua aucuaccugg ucaugauccu acugaucgca      600

cccgccuacu cgauucgaug caucggcgua ucgaaccgcg acuucgucga agguaugagc      660

ggcggaaccu gggucgacgu cguacuagaa cauggcggcu gcguuacagu aauggcacaa      720

gauaagccca ccguagacau cgaacucguc accacgaccg uaagcaacau ggcagaaguc      780

cgcucuuacu gcuacgaagc aagcaucagc gacauggcaa gcgacucacg auguccaacc      840

caaggggaag cauaccucga caagcaaagc gauacacaau acguaugcaa aaggacccuc      900

gucgaccgag gcuggggaaa cggaugcgga cucuucggca aaggaagccu aguaacaugc      960

gccaaauuua caugcagcaa gaaaaugaca ggcaaaagua uccagccaga aaaccucgaa     1020

uaccgcauca ugcuuagcgu acacggcagc caacacucag gaaugaucgu caacgacaua     1080

ggacacgaaa ccgacgaaaa cagggcgaaa guagaaguca cacccaacuc cccacgagca     1140

gaagcuaccc ugggcggauu cgggagccug ggccucgacu gugaaccgag aacaggacua     1200

gacuucagcg accucuacua ccucacaaug aacaacaaac acuggcuggu gcacaaagaa     1260

ugguuccaug acauaccgcu cccauggcac gcaggagccg acacaggaac accgcacugg     1320

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg cgaaaaggca gacagucguc     1380

guccugggau cacaggaagg cgcaguacac acagcacucg ccggcgcguu agaagcagag     1440

auggacggug ccaaaggacg acuguuuuca gggcaccuca aaugccggcu aaaaauggac     1500

aaacugcgcc ucaaaggagu aucauacagc uuaugcaccg ccgccuuuac cuucaccaaa     1560

guccccgcag aaacccucca cggcaccgua acaguggaag uacaauacgc agggaccgac     1620

gggccaugca agguaccugc acaaauggcc gucgacaugc aaacacucac acccgucgga     1680

agacuaauca ccgccaaccc aguaauaaca gaaagcacag aaaacagcaa aaugaugcua     1740

gaacuagacc caccauuugg agacagcuac aucgugaucg gagugggcga caagaaaaua     1800

acccaucacu ggcaccgcuc aggcagcaca auaggcaaag ccuucgaagc aacagucaga     1860

ggagcgaaaa gaauggccgu acugggagac acugccuggg acuucggcuc cgucggcggg     1920

guauucaaca gccuuggcaa aggcgugcac cagauauucg gggccgcguu caaaaguuua     1980

uucgggggaa ugaguugguu cucccaaaua cuaauaggca cacuucuagu auggcucgga     2040

cuaaauacga aaaacggaag cauuagccuc acaugcuuag cccucggcgg aguuaugaua     2100

uuccucagua cagcaguguc agccgacgug ggaugcuccg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9526
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-long mRNA Design 3 opt

<400>  9526
gggagaaagc uuaccaugcg caucauaaac gcacgcaaag aaaggaaacg cagaggagcg       60

gacaccucca uaggaauaau aggccuacua cucacaaccg ccauggcggc cgaaaucacg      120

cgacgaggau cugcguacua cauguaccug gaccgcagcg augccggaaa agccaucucc      180

uucgcaacga cgcucggagu caacaagugc cauguacaaa uaauggaccu cggucacaug      240

ugcgacgcca cgaugucaua cgaaugcccg augcucgacg aaggcguaga accagacgac      300

gucgauugcu ggugcaacac aacgagcaca ugggucguau acggcacuug ccaccacaaa      360

aagggagaag cacgccgcag ccgccgagca gucacacugc caucccacag cacgcgcaaa      420

cuacaaacca ggagccaaac guggcuagaa agcagagaau acacgaaaca ccugaucaaa      480

gucgaaaacu ggauauuccg aaaccccgga uucgcccugg uagcaguagc aaucgcaugg      540

cuacuaggcu ccagcacauc ccaaaagguc auauaccuag uaaugauacu acucaucgca      600

ccagccuaca gcauaagaug caucggcgua agcaaccgcg acuucgucga aggaaugucc      660

ggcgggaccu ggguugacgu aguacucgaa cacggcggau gcgucaccgu gauggcacaa      720

gacaaaccca cgguagacau cgaacucguc acaacgacag ucagcaacau ggcggaagug      780

cguucguacu gcuacgaagc cagcauauca gacauggcau ccgacucaag augcccaaca      840

caaggcgaag ccuacuuaga caaacaauca gacacccaau acgucugcaa acggacacuu      900

gucgaccgcg gauggggaaa cggaugcgga cuauucggaa aaggcucgcu agugacaugc      960

gcaaaguuca caugcucaaa gaaaaugaca ggcaaaucca uacaaccaga aaaccuugaa     1020

uaucgaauua ugcucagcgu ccacggcucc caacacucag gaaugauagu caacgacaua     1080

ggacacgaaa cagacgaaaa cagagccaaa guggagguca caccaaacuc ccccagagca     1140

gaagcaacac uaggaggauu cggaucccug ggacuagacu gcgaacccag gacaggccuu     1200

gacuucuccg accucuacua ccucaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccccu gcccuggcau gccggggcag acacagggac accccacugg     1320

aacaacaaag aggcucuggu agaguucaaa gacgcgcacg cgaaaagaca aacagucguc     1380

guccucggcu cccaggaagg cgccguacau acagcacucg cuggcgcgcu agaagccgaa     1440

auggacggcg caaaaggcag acucuucucc ggacaccuaa aaugccgacu caaaauggac     1500

aaacuacgac ugaaaggcgu auccuacagc cuauguacag cagcauucac cuucacuaaa     1560

guaccagccg aaacccuaca cggcacagua accguagaag uccaguacgc aggaacagac     1620

ggccccugca aaguaccagc acagauggca gucgacaugc aaacgcugac cccgguaggg     1680

aggcucauaa ccgccaaccc aguuaucacg gaaucaaccg aaaacagcaa aaugaugcuc     1740

gaacuggacc cacccuucgg cgauucauac auaguaauag gaguaggcga caagaaaaua     1800

acccaccacu ggcacagauc cggcagcacc auaggcaaag ccuucgaggc cacaguacgc     1860

ggcgccaaaa gaauggccgu acucggagac accgcauggg acuucggauc cgucggaggc     1920

guauucaaca gccucggcaa aggaauccac caaauauucg gagccgccuu caaaagccua     1980

uucggaggca ugagcugguu cagccaaauc cuaaucggga cgcuccuagu auggcuaggc     2040

cucaacacca aaaacggaag caucagccua accugucuag cccugggcgg cguaaugauc     2100

uuccuaagca ccgcggucag cgccgacgua ggcugcagcg uugacuuuuc aaaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9527
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-long mRNA Design 3 opt

<400>  9527
gggagaaagc uuaccaugag aauaaucaac gcaaggaagg aaaggaaaag gaggggcgca       60

gacacgagca uagggauaau cggccuacua cugacaacag cgauggccgc cgaaauaacc      120

aggaggggca gugcauacua cauguaccug gacagaagcg acgccgggaa agcgaucucg      180

uucgcgacua accucggcgu caacaagugc cacgugcaaa uaauggaccu cggccacaug      240

ugcgacgcaa ccaugucgua cgaaugcccg augcuagacg aaggagugga acccgacgac      300

guagacugcu ggugcaacac aacgagcacc uggguaguau acggaaccug ccaccacaag      360

aaaggcgaag cgcgccggag ccggcgagcg gucacacucc caagccauag cacacgaaaa      420

cuccagaccc gcagccagac auggcuagaa agcagagaau acacaaagca ccugaucaaa      480

gucgaaaacu ggauauucag aaacccaggg uucgcacuag cagcgguagc caucgccugg      540

cugcuaggca gcuccacuag ccagaagguc auauaccucg uaaugaucuu acucaucgcg      600

ccagcauacu caauaaggug caucggcgug agcaaccgcg acuucgucga aggcaugucg      660

ggaggaaccu gggucgacgu cguccuagaa cacggcggcu gcgucacagu uauggcacag      720

gacaagccca caguagacau agaacucguc acaacaaccg ugagcaacau ggccgaagua      780

cgaagcuacu gcuaugaagc gucaaucuca gacauggcau cagacagccg augccccaca      840

cagggggaag cauaccucga caagcaaagc gacacccaau acguauguaa acgaacccuc      900

gucgacagag gaugggggaa cggauguggc cuauucggaa aaggcagccu cgugaccugc      960

gcaaaauuua ccugcucaaa gaaaaugacg ggaaagagca uccaacccga aaacuuagaa     1020

uacagaauca ugcuauccgu ccauggaucc caacacagcg gcaugauagu caacgacgaa     1080

aacagagcca aaguggaggu cacgccaaac agcccaagag ccgaagccac ccucggcgga     1140

uucggcagcu ugggccuaga uugcgaaccc aggaccggac uagacuucuc cgaucuuuau     1200

uaccugacca ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauccca     1260

cuaccauggc acgcaggagc agacacaggg accccgcacu ggaauaauaa agaagccuug     1320

gucgaguuca aagacgcaca cgcgaaacgc caaaccguag uaguacuagg cucccaagaa     1380

ggugcggucc acaccgcucu agccggagcc cucgaagcag aaauggacgg cgccaaaggc     1440

cgacuguucu caggccaccu aaaaugucga cuaaaaaugg acaaauuacg ccucaaaggc     1500

guaaguuacu cgcucugcac agcggcauuc accuucacca aagugccagc cgaaacuuua     1560

cacggaaccg uaaccgucga aguacaauau gcgggaacag acggacccug caaaguucca     1620

gcucaaaugg cggucgacau gcagacgcuc acccccgucg gccgacucau aaccgccaac     1680

cccgucauaa cugaaagcac agaaaacuca aagaugaugu uagaauuaga cccacccuuc     1740

ggcgacucau acaucguaau aggcguagga gacaagaaaa uaacccacca cuggcaccga     1800

ucagguagca cgauagguaa agcauucgaa gccacaguca gaggcgcaaa acgcauggca     1860

gugcugggcg acacagcgug ggacuucgga uccgucggcg gaguauucaa cucacucggg     1920

aaagggaucc accaaauauu cggagccgca uucaaauccc uauucggcgg aaugagcugg     1980

uucucccaaa uacuaauagg caccuuauug guauggcuag ggcucaacac caaaaacgga     2040

agcauaagcc ugaccugccu agcacucgga ggagucauga uauuccucag caccgcaguc     2100

agcgcagacg ucggaugcag cguggacuuu agcaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9528
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-long mRNA Design 3 opt

<400>  9528
gggagaaagc uuaccaugag aauuaucaac gcccgaaaag aacgcaagcg gcgaggcgca       60

gacaccucaa uaggaaucgu cggccuacua cuuaccacag cgauggcggc agaaaucaca      120

cgacgcggca gugcauacua uauguaccua gaccgcucag acgccggcaa agcaaucagu      180

uucgcaacca cccucggcgu caacaagugc cacguccaaa uuauggaccu aggccacaug      240

ugcgacgcca ccauguccua cgaaugcccc augcuugacg aaggagucga acccgacgac      300

gucgacuguu ggugcaacac cacaagcacc ugggucgucu acggcacaug ucaccacaag      360

aaaggcgaag cgcgaaggag cagacgagcg guaacccuac caucccauuc cacccguaaa      420

cuucaaacac gcagccaaac cuggcucgaa agccgagaau acacaaaaca ccuaaucaaa      480

gucgaaaauu ggauauuccg caacccgggg uucgcucuag ucgccguggc cauagccugg      540

cuccuaggcu ccucaacguc acaaaaggua auauaccucg ucaugauacu ccuaaucgca      600

ccagcguaca gcauccgaug cauaggcguc agcaaccgcg acuucgugga ggggaugagc      660

ggaggcaccu ggguagaugu cguacucgaa cauggaggcu gcgucacagu cauggcacaa      720

gacaagccaa caguggacau agaacucguc acaacgacgg uuagcaacau ggccgaggua      780

cgaagcuacu gcuacgaggc cagcauauca gacauggcca gcgacucaag gugccccacc      840

caaggggaag cauacuugga caaacaaucc gacacccaau acgucugcaa acggacccug      900

guggaccgag gguggggcaa cggcugcggc cucuucggaa aagguucccu cguuacaugc      960

gccaaauuua cgugcagcaa gaaaaugacc ggaaaaucaa uacaaccuga aaaccucgaa     1020

uacagaauaa ugcucagcgu ccacggcucg caacacagcg gaaugaucgu aaacgacacc     1080

ggacacgaaa ccgacgaaaa ccgcgccaaa gucgaaguca caccaaacuc cccacgcgcc     1140

gaagccacac ucggcggauu cgguagccua ggacuggacu gcgaaccacg cacaggacua     1200

gacuucuccg accucuacua ccuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1260

ugguuccacg acauaccccu cccauggcau gcgggcgccg acaccggcac accccacugg     1320

aacaacaaag aagcucuagu agaguucaaa gacgcacacg cuaaacgcca aaccgucgua     1380

guccuuggcu cccaagaagg cgcuguacau accgcccuag cuggugcccu cgaagcagaa     1440

auggacggag ccaaaggacg ccuguucucc ggccaccuca aaugccgccu caaaauggac     1500

aaacuccgcc ucaaaggagu aucauacucc cuaugcaccg cagcuuucac guucacaaaa     1560

gugccagccg aaacacuaca cggaacagug accgucgaag uccaauacgc cggcaccgac     1620

ggacccugca aaguacccgc ccagauggcc guagacaugc agacccuuac ccccgucgga     1680

agacuaauca ccgcaaaccc agucaucacc gaaucaacag aaaauagcaa aaugaugcuc     1740

gaauuagacc cacccuucgg cgacucauac aucgucauug gcguaggcga caagaaaaua     1800

acccaccacu ggcacagguc agguuccacc aucggcaaag cauucgaagc caccguacga     1860

ggcgccaaac gcauggcagu ccuaggagac accgcguggg acuucggcag cgugggagga     1920

guuuucaacu cccucggaaa aggaauccac caaaucuuug gcgcggcauu uaaaagucua     1980

uuuggaggaa ugagcugguu cucacaaauu cugauaggca cccuccucgu cuggcuaggu     2040

cuaaacacca aaaacggcag uaucucacua accugccucg cacuaggagg gguaaugauc     2100

uuccucagua ccgcagucuc cgcagacguu gggugcagcg ucgacuucuc caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9529
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-long mRNA Design 3 opt

<400>  9529
gggagaaagc uuaccaugcg aauaaucaac gcgaggaaag aacgaaagag acggggcgcg       60

gacacaucca uaggcauagu agggcugcug cuaacaacgg cgauggcagc cgaaauaaca      120

agaaggggaa gcgccuacua cauguaucuc gaccgaucgg acgccgggaa agcaaucagc      180

uucgcaacca cccucggagu uaacaaaugc cacguacaaa ucauggaccu aggccacaug      240

ugcgacgcca caaugagcua cgaaugccca augcuggacg aaggcgugga acccgacgac      300

gucgacugcu ggugcaacac gacaagcacc uggguggucu acggcacaug ccagcacaag      360

aagggggaag cccgacgauc cagacgcgcg gucacacugc caagccacuc cacccgcaaa      420

cuacagacaa ggagccaaac cuggcucgaa agccgagaau acaccaaaca ucucauaaaa      480

guagagaacu ggauauuccg aaacccuggg uucgcccucg ucgcgguggc aaucgcaugg      540

cuacuaggau ccagcacgag ccaaaaaguc auauaccuag uaaugauacu cuuaaucgcg      600

ccagcauacu caauccgaug cauaggcguc uccaacagag auuucgucga aggaaugucc      660

ggcggcaccu ggguagacgu cgugcuagaa cauggaggcu gcguaacagu aauggcacaa      720

gacaaaccaa cagucgacau cgagcucgug acaacaaccg uaaguaacau ggcggaagug      780

cgcucguacu guuacgaagc cagcaucucu gacauggcaa gcgauagcag augcccaacg      840

caaggcgaag cguaccucga caagcaaucc gacacacagu auguaugcaa gaggacauug      900

guagacagag guugggguaa cggaugcgga cuguucggaa aaggcucgcu aguaaccugc      960

gccaaauuca caugcaguaa gaaaaugaca ggcaaaagca uacagcccga gaaccucgaa     1020

uacaggauaa ugcucucagu gcacggaagc caacacagcg gaaugauagu aaacgacauu     1080

ggccacgaaa cugacgagaa cagagcaaaa guagaaguga cacccaacag cccaagggcg     1140

gaggcaacac uaggcggauu cggcagccua ggccuagacu gugaaccgcg aacaggccuc     1200

gacuucagcg accuauacua cuuaaccaug aacaacaaac acugguuggu gcauaaagag     1260

ugguuccacg acauaccacu uccauggcac gccggagcag acacagggac cccacacugg     1320

aacaacaaag aagcacuggu cgaguucaaa gacgcgcacg ccaaacgaca gacaguaguc     1380

guccugggaa gucaagaagg cgcgguacac accgcacucg caggcgcccu agaagccgaa     1440

auggacggcg ccaaaggaag gcuguucagu ggccaccuaa aaugcaggcu caaaauggac     1500

aaacuucgac ugaaaggggu aagcuacucg uugugcaccg ccguaugcac agccgccaaa     1560

guacccgcgg aaacacuaca cggcacggug acaguggaag uccaguaugc cgggaccgac     1620

ggacccugca aggucccagc ccaaauggca guagauaugc aaacccucac gcccguaggg     1680

cgacuaauaa ccgcaaaccc aguaauaaca gaaucgacag aaaacagcaa aaugaugcug     1740

gaacucgacc cgcccuucgg agacagcuau auaguaauag gggucggaga caagaaaaua     1800

acacaccacu ggcaccggag cggaagcaca auugggaaag cauucgaagc gacagugcgg     1860

ggagcuaaaa ggauggcggu acuaggagac accgcauggg auuucgggag cgucggcggg     1920

guauucaaca gccuagggaa aggcauacau caaauauuug gcgcagcuuu caagagccua     1980

uucgggggca ugagcugguu cucacaaaua cuaauaggaa cccuccuagu cuggcucggg     2040

cucaacacaa agaauggauc aauaagccua acaugccuug cucucggagg cgugaugaua     2100

uuccuaucaa cagcggugag cgcggacgug ggaugcuccg uagacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9530
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-long mRNA Design 3 opt

<400>  9530
gggagaaagc uuaccaugcg aauaauaaac gcccgcaagg aaagaaaacg caggggcgcc       60

gacgccucaa uaggaaucgu cucacuccua cucacaacag ugauggccgc agagaucacg      120

cgcagaggau ccgcauacua cauguaccua gacagaagcg acgcaggaaa ggcaauaucc      180

uucgucacaa cccucggcgu gaacaaaugc cacguccaaa uaauggaccu aggccacaug      240

ugcgacgcca caaugucaua cgagugccca augcuggacg aaggcgucga accggacgac      300

gucgacugcu ggugcaacac aacguccaca uggguagucu acggaaccug ccaccacaag      360

aaaggcgaag cacggaggag ccggcgcgca guaacauuac caucacacag uacccgaaaa      420

cuccaaacca gaucccaaac cuggcuagag agccgcgaau acaccaaaca ccuaaucaaa      480

guggaaaacu ggauauucag aaaucccgga uucgcacucg ucgccguagc caucgcaugg      540

cuacugggau ccagcacaag ccaaaaaguu aucuaccucg uaaugauacu ccugaucgca      600

cccgcauacu caauaagaug cauaggcgua agcaaccgcg acuuuguaga aggaaugagc      660

ggagggacgu gggucgacgu cguacuagag cacggaggau gcgucaccgu gauggcccag      720

gacaaaccca ccgucgacau ugaacucguc acgaccacag uaagcaacau ggccgaagua      780

cggucauacu gcuacgaagc cucaauaagc gacauggcau cagacucacg cugcccaaca      840

caaggggaag ccuaccuaga uaagcaaucc gacacccaau acguaugcaa acgcacccua      900

gucgaccgcg gaugggggaa cggaugcgga cuauucggca aaggaucgcu ggugaccugc      960

gccaaauuca caugcucaaa gaaaaugacc gggaaaagca uccaaccaga aaaccucgaa     1020

uaccguauaa ugcuaucagu ccacggcucu caacauucag gcaugaucgu caacgacgag     1080

aaccgcgcaa aaguagaggu gacccccaac uccccaagag cagaggcgac ccucggagga     1140

uucggcucac ugggccuaga cugcgaaccc cgcaccggcu uagacuucag cgaccucuac     1200

uaccuaacca ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauacca     1260

cuucccuggc auucaggggc cgauaccgaa accccgcacu ggaacaauaa agaagcccuc     1320

guagaguuca aggacgcgca cgccaagaga cagacagucg uaguccuugg aagccaggaa     1380

ggcgccguac acacugcccu cgccggagca uuagaagccg aaauggacgg cgcgaaaggc     1440

cgacuaucau cuggccaccu gaaaugccgg cucaagaugg acaagcugcg ucucaaggga     1500

gugucauacu cacucugcac agccgcauuc acauucacca agguccccgc ugaaacccuc     1560

cacggaaccg ugaccgucga aguccaauac gccggacgag acggaccuug caagguuccc     1620

gcccagaugg ccgucgacau gcaaacccua acacccgucg gcagacuaau caccgccaac     1680

cccgucauca cagaaucaac agaaaacuca aagaugaugc uagaacuaga cccaccauuc     1740

ggggacagcu acauaguaau aggcgucgga gacaagaaaa ucacgcacca cuggcaccgc     1800

agugggucaa uaauaggcaa agcguucgaa gccacggucc gcggagccaa acgcauggca     1860

guacuaggcg acaccgccug ggacuucgga agcgucggag gcguguucaa cucacucggc     1920

aaaggaauac aucaaauauu cggcgcugcc uucaaaucuc uauucggagg uauguccugg     1980

uucucacaaa uacuaaucgg aacccuccuc gucuggcucg gacuuaacac caaaaacggg     2040

agcaucucac ugaccugccu cgcccucggc ggcguaauga ucuuccuaag cacagcagua     2100

uccgcagacg ucggcugcag cguagacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9531
<211>  2347
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-long mRNA Design 3 opt

<400>  9531
gggagaaagc uuaccaugcg caucauaaac gcccgcaaag aaagaaaaag aagaggagcc       60

gacaccagua ucggaauagu uggacuccuc cuaacaaccg ccauggccgc cgaaauaacc      120

cgccgcggca gcgcuuacua cauguaccuc gacaggagcg augcaggcaa agccaucuca      180

uucgccacaa cuuugggcgu aaacaaaugc cacgugcaaa ucauggaccu cggccacaug      240

ugcgacgcca cuauguccua cgaaugcccc augcucgacg aaggcguaga accugacgac      300

guagacugcu ggugcaauac aaccagcacc uggguagucu auggaacaug ccaccacaag      360

aaaggugaag cacgccgauc acggagagcc guaacccuac ccucucacag cacccgcaaa      420

cuacaaaccc gcucccaaac auggcuagag ucacgcgagu acaccaaaca cuuaaucaaa      480

guggaaaacu ggaucuuccg uaaccccggc uucgcccucg ucgcagucgc cauagccugg      540

cuccucggcu cauccacauc ccaaaaaguc auauaccuag uaaugauacu ccuuauugca      600

ccagccuaca gcaucagaug caucggcguc ucaaaccgag auuucgucga gggcaugucu      660

ggaggaaccu gggucgacgu aguccucgaa cacggaggcu gcgucaccgu aauggcccaa      720

gacaaaccaa caguugacau agagcuagug accaccacag ugagcaauau ggccgaaguu      780

cgauccuacu gcuacgaagc cagcaucuca gacauggcca gugacucucg cugccccacc      840

caaggagagg ccuacuuaga caaacaaagc gacacacaau acgucugcaa gcgcacccuc      900

gucgaccgcg gauggggaaa cgguugcgga uuauucggca aaggcucucu cguuaccugc      960

gcgaaauuca ccuguuccaa gaaaaugacc ggcaaaagca uccaacccga aaaccuagaa     1020

uacaggauaa ugcucagcgu ccacggcucg caacacuccg gaaugaucgu caacgaugaa     1080

aaccgggcca aagucgaagu cacgccgaac ucaccaagag cugaagcuac acuaggcggc     1140

uucggaagcc ucggccugga cugcgaaccc cgcacaggcc ucgacuucuc agaccucuac     1200

uaccucacca ugaacaacaa acacuggcuc guacacaaag aaugguucca cgacauaccc     1260

cucccauggc acgcaggcgc agacacaggc acaccccacu ggaacaacaa agaagcacuc     1320

guggaguuca aagacgccca cgcaaaacgg caaacagucg ucguacuagg cagccaagaa     1380

ggcgcugucc acaccgcacu ugcaggcgcc cucgaagccg aaauggacgg agcuaaaggc     1440

agacucuuca gcggccaccu aaaaugccgc cucaagaugg acaaacugcg acuuaaaggc     1500

gucucguacu cccuaugcac agcagccuuc accuucacaa aggucccggc ggaaacacua     1560

cacgggaccg uaaccgucga aguacaauac gcaggaaccg acggccccug caaaguaccc     1620

gcacaaaugg cggucgacau gcaaacacug acccccgucg gacgccugau aaccgccaac     1680

cccgucauaa ccgagucaac agaaaacagc aaaaugaugc ucgaacuaga cccucccuuc     1740

ggcgauucuu acaucgucau aggagucggc gacaagaaaa ucacacacca cuggcacaga     1800

uccgguucaa caaucggcaa agcauucgag gccaccgucc gcggcgccaa acgcauggca     1860

gucuuaggcg acaccgccug ggacuucggc agcguaggcg gaguauucaa cagucucggc     1920

aaaggcaucc accaaauauu uggcgccgcg uucaaaagcc uguucggggg cauguccugg     1980

uucucccaaa uccucauagg aacccuccua guauggcuug gacuaaacac caaaaacggc     2040

uccauauccc uaaccugccu cgcucucggc ggggugauga uauuccucag cacagcagug     2100

uccgccgacg ucggcugcuc cguugacuuc uccaagugag gacuaguuau aagacugacu     2160

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2340

cagaauu                                                               2347


<210>  9532
<211>  2365
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-long mRNA Design 3 opt

<400>  9532
gggagaaagc uuaccaugcg aaucauaaac gcaagaaaag aaagaaaaag gagaggcgcc       60

gacaccucaa ucgguauaau cggccuacuc cucacaacgg ccauggccgc agaaaucaca      120

agacgaggcu ccgcauacua cauguaccuc gaccguucag acgcgggcaa agcaauauca      180

uucgccacaa cccucggagu aaacaaaugc cacguccaaa uaauggaccu aggacacaug      240

ugcgacgcca cuaugagcua cgaaugccca augcuagacg aaggagucga acccgacgac      300

gucgacugcu ggugcaacac caccucgacc uggguagugu acgggaccug ccaccacaaa      360

aagggggaaa cccgaagauc ccggaggagu gugagccuaa gguaccacua cacacgaaaa      420

cuccagacca gaagccaaac cuggcuggaa uccagagaau acaagaaaca ccucauaaug      480

guagaaaacu ggauauucag aaacccagga uucgcgauag ucagcguggc aaucacaugg      540

cuaaugggaa gccucaccuc ccaaaaagua auauacuuag ugaugaucgu ccucauagua      600

ccagccuaca gcaucagcug caucggaguc agcaaccgcg acuuagucga ggggaugucg      660

ggcgggacau gggucgacgu aguacucgaa cauggaggcu gcguaacaga aauggcacaa      720

gacaaaccca ccguagacau cgagcucgua accaugacag ucucaaacau ggccgagguc      780

cggucguacu gcuacgaggc gucccuguca gauauggcaa gcgccuccag augcccuacc      840

caaggcgaac caagucucga caaacaauca gacacccaaa gcguaugcaa aagaacacuc      900

ggagacaggg guuggggaaa uggcugugga auauucggaa agggaagccu aguaacaugc      960

aguaaguuua ccugcugcaa gaagaugcca ggaaaaucaa uacaaccaga aaaccuggaa     1020

uaccgaauaa ugcuccccgu acacgguuca cagcacagcg gcaugaucgu aaacgacauu     1080

ggacacgaaa cagaugaaaa cagggccaaa guggaaguga caccgaacag cccacgcgca     1140

gaagccaccc ucggaggcuu cggcucacuc ggacuggacu gcgaaccacg uacggggcuu     1200

gacuucagcg accucuacua ccugacgaug aacaacaaac acuggcuagu acacaaagag     1260

ugguuccaug acauaccccu acccuggcac gccggcgcag auacaggcac gccucacugg     1320

aacaacaagg aggcacucgu cgaguucaaa gacgcccacg caaaaaggca aaccguaguc     1380

gugcucggcu cccaggaagg agcaguacau acagcacugg ccggagcccu agaagcagaa     1440

auggacggag ccaaaggacg ccucuucuca ggccaccuga aaugcaggcu caaaauggac     1500

aaacugagac ucaagggcgu aagcuacagc cucugcacag cagccuucac auucaccaaa     1560

gugcccgcag aaacacucca cggaacgguc accgucgaag uacaauccgc cggaacagac     1620

ggccccugca aaguacccgc acaaauggcu gucgacaugc aaacauuaac acccgucgga     1680

cgacugauca ccgcaaaucc gguaaucacc gaauccacag aaaauagcaa aaugaugcuc     1740

gaacucgacc cacccuucgg agacagcuac auagucaucg gcgucgguga caaaaagauc     1800

acccaccacu ggcacaggag cggcagcaca auaggaaaag ccuucgaagc aaccgugcga     1860

ggagcgaaaa gaauggcagu guuaggcgac accgccuggg acuucggcuc aguaggagga     1920

guguucaaca gccuaggaaa aggaauacac caaaucuucg gcgcggcauu caaaagucuu     1980

uucggaggaa ugagcugguu cagccagaua uuaauaggca cccuccucgu auggcuaggu     2040

cuaaacacca aaaacggcuc caucucacua accugccucg cgcuaggcgg agucaugauc     2100

uuccucagca cagccgucuc cgcagacgua gggugcucag ucgacuucag caaaugagga     2160

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     2220

ccgagauuaa uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2340

ggcucuuuuc agagccacca gaauu                                           2365


<210>  9533
<211>  2359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-long mRNA Design 3 opt

<400>  9533
gggagaaagc uuaccaugcg cauaauaaac gcccgaaaag aacgcaaacg aagaggcgcc       60

gacacaucaa uaggaauaau aggccuccuc uuaacgacag cgauggcagc cgaaauaacc      120

aggagggguu cagccuauua cauguaccug gaccgcagcg acgcaggaaa agccaucucg      180

uucgcaacaa ccaugggagu caacaagugc cacguacaaa ucauggaccu aggacacaug      240

ugcgacgcca caauguccua cgaaugcccu augcuagacg aaggcguaga acccgacgac      300

gucgacugcu ggugcaacac caccagcaca ugggugguau acggaaccug ccaccacaag      360

aaaggagaag cgagaccguc cagacgagcg guaacacugc cgagccacuc cacccgcaaa      420

cuucaaacca ggucacaaac auggcucgaa agcagagaau auacaaagca ccuaaucaaa      480

gucgaaaacu ggauccuccg caacccagga uucacagugg cagcacuagc aauggccugg      540

cuacugggaa acagcaccuc acaaagaguc aucuaccuag ucaugauccu acuaaucgcc      600

cccgccuacu cgaucaggug caucggcguu agcaaccggg acuucguaga aggcaugagc      660

ggcggaacau gggucgacgu agugcucgaa cacggcgggu gcgucacagu aauggcacaa      720

gacaaaccca cagucgauau cgagcuaguc accacaacag uaagcaacau ggccgaaguc      780

aggucguacu gcuacgaagc aagcauaagc gacauggcaa gugacagcag augccccacc      840

caaggggaag ccuaccuaga caaacaaucc gacacccaau acgucugcaa gcgcacccua      900

gucgacagag gguggggaaa cggcugcggc cuauucggca aaggaucccu aguaacaugc      960

gcaaaauuua ccugcagcaa aaagaugaca ggcaaaucaa uccaacccga aaaccucgaa     1020

uacagaauca ugcucucagu ccacgguuca caacacucgg ggaugaucgu caacgauauc     1080

ggacacgaaa ccgaugaaaa cagagcaaag gucgaaguca cuccaaacuc cccuagagcc     1140

gaagcaaccc ucggaggauu cggcucccuc ggccuagacu gcgaaccacg caccgggcuc     1200

gacuucagcg accuauacua ccuaacaaug aacaacaaac acuggcuggu acacaaagaa     1260

ugguuccacg acauaccacu accuuggcac gcaggggccg acaccggcac accccacugg     1320

aacaacaaag aagcgcuagu agaguucaag gacgcacacg caaaaagaca aaccgucgua     1380

guacuaggaa gccaggaagg cgcgguccac accgcgcugg ccggcgcacu agaagccgaa     1440

auggauggag caaaagggcg ccucuucucc ggccaccuca aaugccggau caaaauggac     1500

aaguuaagac uaaaaggcgu cucauacagc cuuugcacag cagccuucac auucucgaaa     1560

gugccggcag agacacugca cggcaccgua accgucgaag uacaauaugc aggcagagac     1620

ggcccaugca agguacccgc acaaauggca gucgacaugc agacacuaac cccagucggc     1680

cgccuaauaa cagcaaaccc caucaucaca gaaagcacag aaaacucccg caugaugcuc     1740

gaacuagacc cacccuucgg cgacagcuac auaguaaucg gggucggcga caagaaaaua     1800

acacaccacu ggcaccgaag ugggagcaca aucggaaaag cauucgaagc cacagucaga     1860

ggagccaaac ggauggcagu ccucggcgac accgcauggg acuucggaag cgucggaggc     1920

gucuucaacu cauugggcaa aggaguccac caaguuuuug gggccgcauu caaaagccug     1980

uucggaggga ugagcugguu cucgcaaaua cuaaucggca cccugcucgu cuggcucggc     2040

cucaacacaa agaacggauc uaucucacuu gcaugccucg cacucggcgg cgccaugauc     2100

uuccuaucca cggcagucag cgccgacgua ggcuguagcg uagacuucug aggacuaguu     2160

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2220

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2340

uuucagagcc accagaauu                                                  2359


<210>  9534
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 prME-short mRNA Design 3 opt

<400>  9534
gggagaaagc uuaccaugac aucaguagga auaguaggcc ugcuacucac caccgcaaug       60

gccgcggaag ucaccagaag agggagcgca uacuacaugu accuagacag gaacgacgcg      120

ggagaggcca uaucauuccc aacuacgcua gguaugaaca aaugcuauau ccaaauaaug      180

gaccuagggc auaugugcga cgccacgaug aguuacgagu gccccaugcu agacgaaggg      240

gucgaaccgg acgacguaga cugcuggugc aacacaaccu caaccugggu agucuacggg      300

accugccacc acaagaaagg cgaagcacgc cgaucaagac gagcgguaac ccucccaagc      360

cacucgacaa gaaaacucca aacccgaagu caaaccuggc ucgaaucucg cgaauacacc      420

aaacaccuca uucgaguaga aaacuggauc uuccggaacc caggcuucgc guuagccgcc      480

gccgccauag cauggcuacu uggaagcucc accucccaaa aagucauaua ccuaguaaug      540

auccuccuca uagcgccagc cuacuccauu agaugcauag gcguaagcaa uagagacuuc      600

gucgaaggca ugagcggggg cacguggguu gacaucgucc uggagcacgg cggcugcgua      660

acaguaaugg cccaagacaa accaaccguu gacaucgagc uagugacgac aaccguauca      720

aacauggcag aaguaagguc cuacugcuac gaagcaagca ucuccgacau ggcaucagac      780

agcagaugcc cgacacaagg agaagcauac cuagacaagc aaagcgacac ccaguacgua      840

ugcaaaagaa cgcuggucga ccgcggaugg ggaaacggau gcggccuauu uggcaaagga      900

agccuaguaa ccugcgccaa auucgcaugc uccaaaaaga ugacaggcaa auccauccaa      960

ccagaaaacc uagaauaucg aaucauguua agcguacacg gaagucaaca cucaggcaug     1020

aucgucaacg acacaggaca cgagacagac gaaaacagag caaaagucga aaucacacca     1080

aauucacccc gagcagaagc cacccuaggg ggcuucggau cgcucggccu agacugugaa     1140

ccacgcaccg gacuggauuu cagcgaccua uacuaccuaa ccaugaauaa caagcacugg     1200

cucguacaca aagaaugguu ccacgacaua ccacucccau ggcacgccgg cgccgacaca     1260

gggacgccac auuggaacaa caaagaagca cucguagaau uuaaagacgc ccacgccaaa     1320

cgacaaacag ucguggucuu ggggucccaa gaaggcgcgg uccacacagc acucgccggc     1380

gcccuggaag cggaaaugga cggcgccaag ggcagacugu caucgggcca ccuaaaaugc     1440

cgacuaaaga uggacaaacu ccgccucaaa ggcguaucau acucccucug cacggcagca     1500

uucacauuca ccaaaauacc cgcggaaaca cuacacggca ccgucacagu agaaguccaa     1560

uacgccggaa cggacgggcc guguaagguc cccgcccaaa uggccgucga uaugcaaacc     1620

cugacuccag ucggccgacu aaucacagca aaccccguca uaacugaauc cacagagaac     1680

aguaagauga ugcucgagcu cgacccaccc uucggcgaca gcuacauagu aaucggaguc     1740

ggcgagaaga aaauaacaca ccacuggcac cgcagcggau ccaccauagg caaagccuuc     1800

gaagccaccg uccgaggugc caaacgcaug gcaguacugg gcgacacagc cugggacuuc     1860

ggcagcgucg gaggagcccu aaacagccug ggaaaaggca uacaccaaau cuucggagcc     1920

gccuucaaau cccuuuucgg aggcaugucc ugguucagcc aaauccugau cggcacccug     1980

cuuauguggc uaggccuaaa caccaaaaac ggauccauca gccuaaugug ccuggcccua     2040

ggcggugugc uaaucuuccu aagcacugcc guaucagcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9535
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 prME-short mRNA Design 3 opt

<400>  9535
gggagaaagc uuaccaugac uucagucggc aucguaggcc uacuccucac caccgccaug       60

gcagccgaag uaacccgccg cggcuccgca uacuacaugu accucgacag gaacgacgcu      120

ggcgaagcca ucuccuuccc cacaacccuc ggaaugaaua aaugcuacau ccaaaucaug      180

gaccucgggc acacaugcga cgcaacuaug uccuaugaau gccccaugcu agacgaaggc      240

gucgaacccg augacgucga uugcuggugc aacacaaccu caaccugggu agucuacgga      300

accugccacc auaagaaagg cgaagcccgc agaucccgac gcgccguaac acuaccgucc      360

cacuccacgc gcaaacucca gacaaggagc cagacauggc uagaaagccg cgaauacacc      420

aaacaccuaa uaagaguaga gaacuggauc uuccgaaacc caggauucgc acucgcagca      480

gcagccaucg cauggcuacu cggauccagc accucccaaa aaguaauuua ccuggucaug      540

auccugcuaa uagcgcccgc auacagcauc aggugcauag gggucuccaa uagagacuuc      600

guagaaggca ugagcggcgg aacgugggua gacgucguac ucgaacacgg cggcugcguc      660

acuguaaugg cccaagacaa accuacgguu gacaucgaac ucguaacaac aaccgucuca      720

aacauggccg aaguacgauc cuacugcuac gaagccucaa uauccgacau ggccuccgac      780

agccggugcc ccacgcaagg cgaagcauac uuagacaaac aaagcgacac acaauacguc      840

ugcaaacgaa cacuggucga ccgcggcugg ggaaacggcu guggacuauu cggaaaaggc      900

ucacucguaa caugcgcaaa auucgccugu agcaagaaaa ugacaggaaa aagcauccaa      960

cccgaaaacc ucgaauaccg aaucaugcug ucaguccacg gaagccaaca uucaggaaug     1020

aucgucaacg acacaggaca cgaaacugac gaaaacagag ccaaggucga gauaacacca     1080

aacagcccac gugcagaagc cacccuagga ggguucgggu cacucggccu ugacugcgaa     1140

ccgcggacgg gacuagacuu cagcgaccuu uacuaccuca caaugaacaa caaacacugg     1200

cuaguucaua aagaaugguu ccacgauauc ccacugcccu ggcacgcagg cgccgacaca     1260

ggcacaccac acuggaacaa caaggaagcc cucgucgagu ucaaagacgc ccacgcgaaa     1320

agacaaaccg ucgucguacu aggcucacaa gagggcgccg uccacaccgc ccuagccggc     1380

gcccuagagg cagaaaugga cggcgcaaag ggccgccucu cauccgggca ccucaaaugc     1440

cgccucaaaa uggacaaacu ccgucuuaaa ggcgucucgu auucccuaug caccgccgcc     1500

uucacguuca ccaaaauacc ggcagaaacg cuccacggaa ccgucaccgu agaaguacag     1560

uaugccggca cggacggccc cugcaaaguc ccagcccaaa uggccgucga caugcagaca     1620

cucaccccag ucggcaggcu aaucacggcg aaucccguca ucacagaauc cacagaaaac     1680

ucaaaaauga ugcuagaacu agacccgcca uucggcgaca guuacaucgu caucggaguc     1740

ggcgaaaaga agaucacaca ucacuggcac agaagcggca gcacaauagg gaaggccuuu     1800

gaagcaaccg ucagaggugc caaaagaaug gcaguccucg gcgacaccgc cugggacuuc     1860

ggaucgguag gcggugcccu caacucccua ggaaagggca uccaccaaau cuucggugcc     1920

gcauucaaau cccuauucgg gggcauguca ugguucucac agauacuaau aggaacccuc     1980

cucauguggc uaggccuaaa cgccaaaaac ggcagcauaa gccugaugug ccuagcccuc     2040

ggcggcguac uaaucuuccu cagcacagcc gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9536
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 prME-short mRNA Design 3 opt

<400>  9536
gggagaaagc uuaccaugac gagcauaggc auaauaggac ucuugcucac aaccgcaaug       60

gccgccgaaa ucacgcgacg aggcucagcc uacuacaugu accuagacag aagcgacgca      120

ggcaaagcca ucucguucgc aaccacacuc ggaguaaaca aaugccacgu acaaauaaug      180

gaucucggac acaugugcga ugcaacaaug uccuacgagu gccccauguu agacgaagga      240

guagaaccgg acgacgucga cugcuggugc aauaccacaa gcacgugggu aguauauggc      300

acgugccacc acaagaaagg cgaagcacgc agaagccggc gcgccgucac gcuacccagc      360

cauagcacca gaaagcucca gacacggucc caaaccuggc ucgagagccg cgaauauaca      420

aaacaccuga ucaaggucga aaacuggauc uuccggaauc cuggauucgc acucgucgcc      480

guugcaaucg cuuggcuccu aggcucgagc acgagccaaa aaguaaucua ccucguaaug      540

auacuacuaa ucgcgcccgc auauucuauc cgaugcauag gcgugagcaa ccgugacuuc      600

gucgaaggaa ugucgggcgg gacauggguc gauguaguac uagagcacgg gggaugcguc      660

acggucaugg cacaagacaa gcccaccguc gauaucgagc ucgucacgac cacagugagc      720

aacauggcug aagugcgcuc auacugcuac gaagccucca uaucagacau ggcauccgac      780

agcagaugcc cuacacaagg ugaagcauac cuggacaaac aaucagacac acaauacguc      840

ugcaaaagga cccuagucga ucgcggaugg gggaacgggu gcggacuauu cggcaaagga      900

agccuaguga ccugcgcuaa auuuaccugc ucgaagaaaa ugacaggaaa gagcauccaa      960

cccgaaaacc ucgaauacag aaucaugcuc ucaguccacg gcucucaaca uagcgggaug     1020

auaggcuacg aaacugacga ggacagagcc aaagucgagg ucacgcccaa cagcccgcgc     1080

gcagaagcua cacugggagg auucggcagc cuaggacuag acugcgaacc acgcacagga     1140

cucgacuuca gcgaccuaua cuaccugaca augaacaaca aacacuggcu aguccacaaa     1200

gaaugguucc acgauauacc gcucccgugg cacgccggcg ccgacaccgg uaccccacac     1260

uggaacaaca aagaagcacu cgucgaguuc aaagacgcac acgcaaaaag acaaacgguc     1320

guaguauuag gcucgcaaga aggcgcaguc cacacagccc uagcaggcgc gcuagaagca     1380

gaaauggacg gcgcgaaagg cagacuauuc ucaggccauc ucaagugccg ccuaaaaaug     1440

gacaaacucc gccuaaaagg gguauccuac ucacucugca cagcagcauu cacauucacc     1500

aaaguccccg cggaaacccu acauggcaca guaaccguag aagugcaaua ugccggaacg     1560

gauggccccu gcaagauccc gguacaaaug gcgguugaca ugcagacucu gaccccagua     1620

gggcgccuaa ucacagcaaa cccuguaauc acugaaucca cagaaaacuc aaaaaugaug     1680

cucgaacugg acccaccauu cggggacagc uacauaguaa uaggagucgg cgacaagaaa     1740

aucacccacc acuggcacag aagcgggagu acaaucggaa aagcguucga ggccacaguc     1800

aggggcgcaa aaagaauggc aguacuagga gacaccgcuu gggacuucgg cagcgugggc     1860

gggguauuca acucacucgg aaaaggcauc caccaaaucu ucggugcagc cuucaaaagc     1920

cuguucggag gaauguccug guucucccaa auucucaucg gaacgcuccu agucuggcuc     1980

ggacuaaaua ccaaaaacgg aucuaucagc uuaaccugcc ucgcgcuagg aggagugaug     2040

aucuuccugu ccacagcagu guccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9537
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 prME-short mRNA Design 3 opt

<400>  9537
gggagaaagc uuaccaugac cuccguaggc aucguaggcc uacuccugac gaccgccaug       60

gccgccgaag ucacccgccg cggcuccgca uacuacaugu accucgaccg aaacgacgcc      120

ggagaagcca ucagcuuucc cacuacccuc ggcaugaaca aaugcuacau ccaaaucaug      180

gaccucgguc acaugugcga cgcuacuaug agcuacgaau gucccaugcu agacgaggga      240

guugaacccg acgacgucga cugcuggugc aacacaacau caacaugggu cguauacggc      300

accugucacc acaagaaagg cgaagcaaga cguucuagac gagccguaac acuaccaucc      360

cauucgaccc gcaaauuaca aacccgcucc caaaccuggc uagaaucacg agaauacacc      420

aaacaccuca uccgcgucga gaacuggauc uucaggaacc cgggauucgc acuugcugcc      480

gcagcaauag cauggcuccu cggauccucc accucccaaa aaguuauaua ccucgucaug      540

auccuccuua ucgcccccgc auacucgauc cgcugcauag gugucucaaa ccgagacuuc      600

guggaaggca uguccggggg cacgugggua gacguggucu uagaacacgg cggcugcgua      660

accguaaugg cacaagacaa accaacagug gacaucgaac uggucaccac aacaguaucc      720

aacauggccg aaguccgcag cuauugcuac gaagcaagca uaucugauau ggcguccgac      780

ucacgcugcc ccacucaggg cgaagccuac cuugacaagc aauccgacac ccaauacguc      840

ugcaaacgca cacucgucga ccgaggcugg ggcaacggau gcggacuauu cggaaagggc      900

ucccuaguca caugcgccaa auucgccugc uccaagaaaa ugaccggcaa aucgauacaa      960

cccgaaaacc ucgaauaccg uauaaugcua uccguccacg gcucacaaca uucagggaug     1020

aucgucaacg acacaggcca cgaaaccgac gaaaaccgag ccaaggucga aauaaccccg     1080

aacagcccca gagccgaagc cacccuagga ggcuucggcu cccuaggccu agacugcgaa     1140

ccccgcaccg gccucgacuu cuccgacuug uacuaccuca cgaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacaua ccccucccau ggcacgccgg cgcagauacc     1260

ggaacucccc acuggaacaa caaagaagcc cuagucgagu ucaaagacgc ccacgccaaa     1320

cgccaaacag ucguaguccu aggaucccaa gaaggcgccg uucacacggc acucgccgga     1380

gcacuagaag ccgaaaugga cggcgcaaaa ggccgucuau ccucaggcca ccuuaaaugc     1440

cgccugaaga uggauaaacu ccggcucaaa ggagucuccu acucacugug caccgccgca     1500

uucacauuca ccaaaauccc agccgaaacc cuccacggca caguaaccgu ugaaguccaa     1560

uacgccggca ccgauggccc augcaaagua ccggcccaaa uggcaguaga caugcaaaca     1620

cucacacccg uaggacgccu cauaacggcc aacccaguaa uaaccgaauc cacagaaaac     1680

uccaaaauga ugcuagaacu agacccaccc uucggggacu ccuacaucgu caucggcgua     1740

ggcgaaaaga aaaucacaca ucacuggcac cgcucaggau caacaauagg aaaagccuuc     1800

gaagccacag uaagaggugc aaaacgcaug gcaguacuag gagacacagc augggacuuu     1860

gggucagucg gcggagcccu gaacucacuc ggaaaaggga uccaccaaau cuucggcgcc     1920

gcauucaaau cccuauucgg aggaaugagc ugguucagcc aaauccucau cgggacccua     1980

cuaauguggc ucggccucaa caccaaaaac ggcuccaucu cccuuaugug ucucgcucua     2040

ggcggcguac ucauauuccu cagcaccgcc gucucugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9538
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 prME-short mRNA Design 3 opt

<400>  9538
gggagaaagc uuaccaugac gagcaucggc aucauagguc uccuccuaac cacggccaug       60

gccgccgaaa ucacccgccg cggcuccgca uacuacaugu accuugaccg cucagaugcc      120

ggaaaagcaa ucucauucgc gacaacccuc ggaguaaaca aaugccacgu acaaaucaug      180

gaccugggcc auaugugcga cgccacgaug uccuacgaau gccccaugcu agacgaaggg      240

gucgaacccg augacguaga uugcuggugc aacacaacaa gcaccugggu cgucuacggc      300

acaugccacc acaagaaagg agaagcacgc cgaucacgcc gagccgucac cuuacccucu      360

cacucaaccc gaaaacuaca gacaagaucc cagaccuggc uugaaagccg cgaauacaca      420

aaacaccuca ucaaagucga aaacuggaua uuccggaacc ccggauucgc ccuaguagca      480

guagcaauag cauggcugcu cggauccuca acaucacaaa aaguaaucua ccucgucaug      540

auccugcuua ucgcacccgc cuacucaauc agaugcauag gagucagcaa ccgcgacuuc      600

gucgaaggga ugucaggagg cacguggguc gacguagucc uagaacacgg cggcugcguc      660

acaguaaugg cccaagacaa accuaccgua gacaucgagc ucguaaccac cacuguaucc      720

aauauggccg aaguccguuc auauugcuac gaagccucaa ucuccgacau ggcaagcgac      780

ucccgaugcc ccacccaagg agaagcguac cucgacaaac aauccgacac ccaguacguc      840

ugcaaacgaa cuuugguaga ccggggcugg ggcaacggau gcggucucuu cgguaaagga      900

uccuuaguaa ccugcgcaaa auuuaccugc agcaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaacc ucgaauaccg caucaugcuc ucaguccacg ggucccaaca cuccggaaug     1020

auagucaaug acacaggcua cgaaaccgac gaaaaccgcg ccaaaguaga agucacaccc     1080

aacagccccc gcgcagaggc cacccucggc ggcuucggcu cccuaggccu agacugugaa     1140

ccgcgaacag gccuggacuu cuccgaccuc uacuaccuua cuaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacauc ccucuaccau ggcacgccgg agcagacacc     1260

ggcacacccc auuggaacaa caaagaagcc cuuguugagu ucaaggacgc ccacgcgaaa     1320

cgacaaaccg ugguaguacu ugguucacaa gaaggcgcag uccacacagc ccuagccgga     1380

gcccucgaag cagagaugga cggagccaaa ggcaaacucu ucuccggcca ccuaaagugc     1440

agacuaaaaa uggacaaacu aagacuaaaa gggguaucau acagccuaug caccgccgcu     1500

uucaccuuca cgaaaguccc agccgaaacc cuacacggca ccguaaccgu cgaaguacaa     1560

uacgcaggaa ccgacggccc cugcaaaauc cccguccaga uggccguaga uaugcaaaca     1620

cuaacccccg ucggacgccu aauaaccgcc aacccaguca ucaccgaaag cacugaaaac     1680

uccaaaauga ugcuagaacu cgacccgcca uucggcgaca gcuacaucgu aaucggaguc     1740

ggggauaaaa agaucaccca ccacuggcac cgcucaggca gcacgauagg caaagccuuc     1800

gaagccacag uccgcggggc caaacguaug gcaguccuag gagacacggc cugggacuuc     1860

ggcuccgucg gcggagucuu caacucccua ggcaaaggca uccaccaaau auucggcgcg     1920

gcauucaaaa gccuauucgg cggcaugucc ugguucucac aaauccuaau aggcacccuc     1980

cuaguauggc uaggacuaaa caccaaaaac ggauccauaa gccuaaccug ccuagcacua     2040

ggcggcguaa ugauuuuccu aagcacggcc gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9539
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX377335.1 prME-short mRNA Design 3 opt

<400>  9539
gggagaaagc uuaccaugac gaguaucggu aucauaggcc uacugcugac aaccgcgaug       60

gcugcagaga ucacacguag aggaagcgca uacuacaugu accuggaccg cagcgacgcc      120

ggaaaagcaa uaucauucgc cacaacgcuc ggaguaaaca aaugccacgu ccagauaaug      180

gaccucgguc acaugugcga cgcaaccaug ucguacgaau gccccaugcu agacgaggga      240

gucgaaccag acgacgucga uugcuggugc aacacaacca gcacgugggu cguguacgga      300

accugccaca gaaagaaagg ggaagcgaga agaagcagac gagcggucac acucccuagc      360

cacagcacac gcaaacucca aacccgaagc caaacguggc ucgaaagccg agaauacaca      420

aaacaccuca ucaaagucga aaacuggauc uuccgcaacc ccggguucgc acucguggca      480

gucgccauag cauggcuccu gggcagcagc acgagccaaa aaguaaucua ccuagugaug      540

auacuccuga ucgccccagc cuacucgaua cgaugcauag gcgucagcaa ccgcgauuuc      600

guggaaggaa uguccggcgg gacgugggua gacguagucc uggaacacgg aggcugcguc      660

accguaaugg cccaggacaa acccacaguc gauauagagc uggugacgac aacaguaagc      720

aacauggcag aaguacgcuc guacugcuau gaagcaucga ucucggacau ggcaagcgac      780

agccgcuguc caacccaagg agaggccuac cuagacaaac agagcgacac acaguauguc      840

ugcaaaagaa cccucguaga cagaggcugg ggaaacggau gcggccucuu cggcaaggga      900

ucgcuaguaa caugcgccaa auucacaugc uccaagaaaa ugacaggcaa aagcauccag      960

cccgaaaacc uagaguaccg aauaaugcug ucaguccacg gcucacaaca cuccggcaug     1020

auaguaaacg acaccggcua cgaaacggau gaaaacagag ccaaagucga gguaaccccg     1080

aacuccccac gagcagaagc aacucucgga ggguucggga gccucggguu agacugcgaa     1140

ccccgcaccg gauuggacuu cagcgaccuc uauuaccuga caaugaacaa caaacacugg     1200

cucguccaca aagagugguu ucacgacauc ccccuccccu ggcacgcagg cgccgacaca     1260

ggaacgccac acuggaacaa caaagaggcc uuggucgagu ucaaagacgc acacgccaaa     1320

agacaaaccg uggucguacu cggcagccaa gaaggagcag uacacacagc gcuagcaggc     1380

gcacuagaag ccgaaaugga cggagcaaaa gggaaauugu ucucuggcca ccucaaaugc     1440

agacugaaaa uggacaaacu aagacugaaa ggagugucau acucacugug cacagcagca     1500

uucaccuuca caaaaguacc agcggaaacc cuucacggga caguaaccgu agaaguccaa     1560

uacgcaggaa ccgacgggcc gugcaagauc ccagugcaga uggccgucga caugcagacc     1620

cuaaccccag ucgggcgacu caucaccgcc aacccaguaa uaacagaaag uaccgaaaac     1680

agcaaaauga ugcuugaacu agacccaccc uucggagaca gcuacauagu aauaggcgug     1740

ggagauaaga aaauaaccca ccacuggcac agaagcggga gcacaauagg aaaagcauuc     1800

gaggccaccg ucaggggagc aaaaagaaug gcaguacucg gagacaccgc cugggacuuc     1860

gggucugucg gaggaguauu caacucacua gggaaaggaa uccaccaaau auucggcgcc     1920

gccuucaaga gcuuauucgg gggcaugucc ugguucagcc aaauccuaau uggaacccuc     1980

cuaguguggc uaggccuaaa caccaaaaac ggaagcauaa gucuaacaug ucuagcccua     2040

ggcggcguga ugauauuccu aagcaccgcg guaagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9540
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 prME-short mRNA Design 3 opt

<400>  9540
gggagaaagc uuaccaugac aagcaucggc auaaucggac uauuacucac aaccgcaaug       60

gcagccgaaa uaacccgccg aggaucugcc uacuacaugu accucgacag gagcgacgcc      120

ggaaaagcaa uaucauucgc aacaacccua gggguaaaca agugccacgu acaaauaaug      180

gaccucggcc acaugugcga cgccacaaug agcuacgaau gccccaugcu ggacgagggc      240

guggaaccgg acgacgucga cugcuggugc aacaccacuu ccaccugggu cguauacgga      300

accugccacc acaagaaagg ggaagcacga agauccagac gagccgucac auuaccaagc      360

cacucaacca gaaaacuaca gacaagaagc caaacauggc ucgaaagcag ggaguacaca      420

aaacaccuga uaaaagucga aaacuggaua uuccgcaacc ccggguucgc ccucguggcc      480

guagcaauag ccuggcuacu aggcuccagc acaagccaaa aagucaucua ccuagucaug      540

auccuccuaa ucgcgccagc auacuccauc cgguguauag gcgucucaaa cagagacuuc      600

gucgaaggga ugucaggcgg aaccugggua gacgugguac ucgagcacgg aggcugcgua      660

acagucaugg cccaagacaa acccaccguc gacaucgaac ugguaacaac caccguaagc      720

aacauggcug aaguccgauc cuacugcuau gaagccagca ucucagacau ggcaagugac      780

ucacgaugcc caacccaagg cgaagccuac cuggacaagc aaagcgacac ccaguacguc      840

ugcaaacgca cccuagucga ccgagggugg ggaaacggcu gcggccucuu cggaaaagga      900

ucgcuaguca cgugugcaaa guuuaccugc uccaagaaaa ugacaggcaa aaguauacag      960

ccugaaaacc ucgaguaucg aaucaugcua ucugugcacg gcucacaaca cucaggaaug     1020

acggucaacg acauaggaua cgagaccgac gaaaaccgcg caaaagucga ggucacccca     1080

aacagcccac gggcagaagc cacccucgga ggguucggca gccuaggccu cgauugcgaa     1140

ccaagaacag gccuugacuu cuccgaccua uacuaucuca ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacaua ccacuccccu ggcacgcagg cgcggacaca     1260

gggacgccac acuggaacaa caaagaagcc cuaguagagu ucaaagacgc acacgccaaa     1320

cgacaaaccg ucgucguucu aggcucccaa gaaggcgcag uccacacggc ccuagcagga     1380

gcgcuagaag ccgagaugga cggcgccaag ggcaaacucu ucagcggaca ccuaaaaugc     1440

aggcuaaaaa uggacaaacu acggcucaag ggaguaucau acucgcugug caccgcggcg     1500

uucaccuuca ccaaaguacc cgccgagacc cuccacggaa ccguaaccgu cgaaguccaa     1560

uacgcaggga cugacggccc gugcaaaauc ccaguccaaa uggcagucga caugcaaaca     1620

cuaacaccag ucgggcgacu caucacagca aaccccguaa ucacagaauc caccgaaaac     1680

uccaagauga ugcucgagcu agacccgcca uucggagaca gcuacaucgu aauaggaguc     1740

ggagacaaaa agaucaccca ccacuggcac cgcucgggaa gcacaaucgg caaggccuuc     1800

gaagcaacug uaagaggcgc aaaacgcaug gccguacuag gagacaccgc uugggacuuc     1860

ggcagcgucg gcgggguguu caacucccua ggaaaaggca uacaccaaau auucggagca     1920

gcuuuuaaau cccuguucgg cggcaugagc ugguucagcc aaauacuaau aggcacccuc     1980

uuagucuggc uaggacucaa cacaaagaac ggcucaaucu cacuaaccug ccucgcacua     2040

ggaggaguca ugauauuccu gagcacagcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9541
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 prME-short mRNA Design 3 opt

<400>  9541
gggagaaagc uuaccaugac aagcauaggc auagucggcc ugcuacucac cacugccaug       60

gccgccgaaa uaacacgcag aggcuccgcc uacuacaugu accucgaccg cuccgacgca      120

ggaaaagcca ucuccuucgc aaccacgcuc ggcgucaaca aaugucacgu acaaauaaug      180

gaccuaggcc acaugugcga cgccaccaug agcuacgaau gccccaugcu cgacgagggc      240

gucgagccgg acgacguaga cugcuggugc aacacaaccu cuacaugggu agucuaugga      300

accugccauc acaagaaagg cgaggcccga cgaagccgac gagcaguuac cuuacccagc      360

cacagcacac gcaaacucca aacaagaagc caaacauggc uagagagccg agaauacaca      420

aaacaccuca ucaaagucga aaacuggaua uuccggaauc caggcuucac acucgucgca      480

guagcaauag ccuggcuccu cggcagcagc accagccaaa aaguaaucua ccuagucaug      540

auccuccuaa uagcgccagc cuacuccaua aggugcauag gaguaagcaa ucgcgacuuc      600

guagaaggaa ugagcggagg caccuggguc gacguagucc ucgaacacgg gggaugcgua      660

acaguaaugg cccaagacaa gccaacagua gacauagagc ucgucacaac gaccgucagu      720

aacauggcag aagucagguc uuacugcuac gaagcaagca ucagcgacau ggcaucagac      780

agccgaugcc ccacccaagg agaagcguac cuagacaagc aaucagacac ccaauacguc      840

ugcaaaagga cccuuguaga uagaggaugg ggaaacggau gcggccucuu cggcaagggc      900

agccuaguaa ccugcgcaaa auucacaugc ucaaagaaaa ugaccggcaa aucaauccaa      960

ccagaaaacc uugaauaccg aaucaugcuc uccguccacg gaagccaaca cagcggaaug     1020

auagucaacg acgaaaacag agcaaaaguc gaagucacuc ccaacucacc ccgagccgaa     1080

gccacccugg gcggcuucgg aucccuggga cuugacugcg aaccucgcac cggccuagac     1140

uucagcgacc uauacuaccu cacaaugaac aacaaacacu ggcuagucca caaagaaugg     1200

uuucacgaca uaccccuccc auggcacgcc ggcgcagaca ccggaacacc ccauuggaac     1260

aacaaagaag cccuagucga guucaaagac gcacacgcaa aacggcagac aguagucgua     1320

cuaggcuccc aggagggcgc aguacacacc gcacuggccg gcgcccugga agccgaaaug     1380

gauggcgcaa aaggaaggcu guucucggga caccucaaau gcaggcugaa aauggacaaa     1440

cuaagacuca aagggguauc auacuccuua ugcacagccg ccuucaccuu caccaaagua     1500

ccagcagaga cgcuccacgg caccgugacc gucgagguac aauacgccgg caccgacgga     1560

ccaugcaaag uccccgccca aauggcagua gauaugcaga cacugacccc agucggcaga     1620

cuaauaaccg ccaacccggu uauaaccgaa agcaccgaaa acucaaaaau gauguuggaa     1680

cuggacccac ccuucggcga uagcuacaua gucauaggug uaggagacaa gaaaaucacc     1740

caccacuggc acagaucagg uagcacaauc ggcaaagcgu ucgaagccac cguccgcggc     1800

gcaaaaagga uggccguacu cggcgacacc gccugggacu ucggcagcgu gggaggaguc     1860

uucaacagcc uaggaaaagg cauccaccaa aucuucggag ccgccuucaa aagccucuuc     1920

ggaggaauga gcugguucuc ccaaauccuc aucggaaccc uacuggucug gcucggccuc     1980

aacaccaaaa acggcagcau cucucuaacc ugccucgcac uaggcggcgu caugauauuc     2040

cucagcaccg cggucucagc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  9542
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 prME-short mRNA Design 3 opt

<400>  9542
gggagaaagc uuaccaugac cucaaucgga aucaucggac uauugcucac caccgcgaug       60

gcagcagaaa ucacacgcag aggauccgca uauuacaugu accuugaccg cagugacgcc      120

ggcaaagcaa ucucuuucgc cacaacacuc ggagucaaca aaugccacgu ccaaaucaug      180

gaccucggcc acaugugcga cgccacaaug uccuacgaau gccccaugcu cgacgaaggc      240

gucgaacccg acgacgucga cugcuggugc aacaccacau ccacuugggu cgucuacgga      300

accugccacc acaagaaagg cgaggcgaga cgcuccagac gcgccgucac acuacccucg      360

cacuccacaa ggaaacucca gacccgaagc cagaccuggc uagagucccg ggaauacacc      420

aaacacuuaa ucaaaguaga gaacuggaua uuuagaaacc ccggauucgc ucucgucgcc      480

gucgcaauug cuuggcuccu aggcucaagc accagucaaa aaguuauuua ccuagucaug      540

auacuacuaa ucgcacccgc cuacuccauc cgcugcaucg gagucaguaa ccgcgacuuc      600

guggaaggca uguccggagg aacguggguc gacguagucc ucgagcacgg aggaugcguc      660

acagucaugg cacaggacaa accaaccguc gacauagagc uagucaccac gacagucuca      720

aacauggccg aaguaagguc uuacugcuac gaagccagca ucuccgauau ggccucagac      780

ucccgaugcc ccacccaagg agaggccuac cucgacaaac aaucagacac ccaauacgua      840

ugcaaaagaa cccuaguuga ccgcggcugg ggaaacggcu gcggccucuu cggcaaaggg      900

agccuaguaa ccugcgcaaa auucacaugc uccaagaaaa ugaccggcaa auccauccaa      960

ccagaaaacc ucgaguaccg cauaaugcuc ucaguacacg gcucacaaca cagcggaaug     1020

auaggcuacg aaaccgacga aaaccgcgcc aaagucgaag ucacccccaa cagcccacgc     1080

gccgaagcca cacucggcgg auucggcucc cuaggccuag acugcgaacc ccgcaccggu     1140

cuugauuuuu cagaccuuua uuaccucaca augaacaaca aacacuggcu cgugcauaaa     1200

gaaugguuuc acgauauacc acuaccgugg caugcgggag cagacaccgg cacgccccac     1260

uggaacaaca aagaagcacu cgucgaguuc aaagacgccc augccaaacg ccaaaccgua     1320

guaguucuag gaucacaaga aggagcaguc cacaccgccc ucgccggagc cuuagaagca     1380

gaaauggacg gagcgaaagg caaacuguuc agcggccacc uaaagugcag acucaaaaug     1440

gacaagcucc gccucaaagg cgucucauac ucacuaugca ccgcagccuu caccuucacc     1500

aaggucccag cagaaacccu ccacgggacc gucacugucg aaguccagua ugcaggcacg     1560

gacggaccau gcaagauccc gguccagaug gcaguagaca ugcaaacccu cacacccguc     1620

ggccgacuca ucacagcaaa ccccgugauc acagaaucca cugaaaacuc aaaaaugaug     1680

cucgaacucg acccgcccuu cggcgacagc uacaucguca ucggcgucgg cgacaaaaag     1740

auaacccacc acuggcaccg cagcggguca acuaucggca aagcauucga agccaccguc     1800

cgcggcgcca aacgaauggc cguacucgga gacacggccu gggauuucgg cuccguaggc     1860

ggaguauuca acagccuagg aaagggcauc caccaaaucu ucggagcagc auucaaaagc     1920

cuauucggcg gcaugucaug guuuucccaa auacugaucg gcacacucuu agucuggcuc     1980

ggacucaaca ccaagaacgg cuccaucagc cuaaccugcc ucgcacuagg cggagucaug     2040

aucuuccuaa gcaccgccgu aagcgcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  9543
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694534.2 prME-short mRNA Design 3 opt

<400>  9543
gggagaaagc uuaccaugac cuccgucgga auagucggcc ucuuacuaac cacugccaug       60

gccgcagaag uaacccgccg agggagcgca uacuacaugu accucgaccg caacgacgca      120

ggagaagcca ucagcuuccc cacgacgcua ggcaugaaca agugcuacau acagaucaug      180

gaccuaggcc auaugugcga cgccacaaug ucauacgaau gcccaaugcu agacgaaggc      240

gucgaacccg acgacgucga cugcuggugc aauacaacaa gcacuugggu agucuacgga      300

accugccacc acaagaaagg ggaagcacga cgaagccgcc gagcagucac ucuccccuca      360

cacagcaccc ggaaacucca aacacgcagc caaaccuggc uagaaagccg agaauauaca      420

aaacaccuca uacgaguaga aaacuggauc uuccgaaacc caggcuucgc auuagccgcc      480

gcggccaucg ccuggcuacu gggcucguca acaucacaga aaguuaucua ccuaguaaug      540

auacuucuaa ucgcccccgc cuacagcaua agaugcaucg gcguaagcaa cagagacuuc      600

guagagggga ugagcggcgg gaccugggua gacgucgucc uggagcacgg cgguugcguc      660

acgguaaugg cccaagacaa accaaccguu gacaucgaac ucguaacgac gaccgugucc      720

aacauggcag aaguccgauc auacugcuac gaagccagca ucagcgacau ggcauccgac      780

ucuagaugcc cgacccaggg agaagccuac cuugacaaac aaagugacac acaauacguc      840

ugcaaacgaa cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cgggaaaggc      900

agccuaguaa ccugugcgaa auucgcaugc agcaagaaaa ugacagggaa auccauacag      960

ccagaaaacc uagaauaccg cauaaugcug aguguccacg gaucucaaca cagcggcaug     1020

aucguaaacg acacaggaca cgaaacagac gaaaaccgag caaaaguaga gaucaccccg     1080

aacagccccc gagcggaagc aacacuaggc ggauucggca gccugggccu cgacugugaa     1140

cccagaacag gcuuagacuu cagcgaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cucguccaua aagaaugguu ccacgacauc ccgcucccau ggcacgccgg cgcagacaca     1260

ggaacgccac acuggaacaa caaagaggcg cuaguggagu ucaaagacgc acacgccaaa     1320

cgccagaccg uagucguacu aggaucacaa gaaggcgccg uucacacagc ccuagccgga     1380

gcacucgaag ccgaaaugga cggcgcaaag ggcagacuau caucaggcca ccuaaaaugc     1440

cgccuaaaga uggacaaacu gcgccuaaaa ggcgugucau acucacuaug cacagcagca     1500

uucacguuua ccaagauacc agcagaaaca cuacacggua cagucaccgu cgaaguccaa     1560

uacgcaggga ccgacggacc augcaaagua cccgcccaaa uggcagugga caugcaaaca     1620

cucacaccgg uaggacgacu uaucaccgcc aacccgguaa uaacagaauc caccgaaaac     1680

agcaaaauga ugcucgaacu cgacccaccc uucggagacu ccuacauagu aauaggcguc     1740

ggagaaaaga aaaucacaca ccacuggcac cgcuccggcu ccaccaucgg gaaagcauuc     1800

gaagccacag ucagaggagc caaacgaaug gcgguccugg gcgacacggc augggacuuc     1860

ggaagcgucg ggggcgcacu caacucacuc ggccguggaa uacaccaaau cuucggggcc     1920

gcguucaaaa gccucuucgg cggcaugagc ugguucucgc aaauccucau aggcacccua     1980

cucauguggc ucggacuaaa caccaaaaac ggaucaauca gccucaugug cuuagcccua     2040

ggcggcgucc ucauuuuucu cagcaccgcg guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9544
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX369547.1 prME-short mRNA Design 3 opt

<400>  9544
gggagaaagc uuaccaugac cuccguagga aucguuggcu uacuccucac caccgcaaug       60

gcagcagaag uaacucggcg cggcucagcc uacuacaugu accucgaccg caacgacgca      120

ggagaagcca uaagcuuccc caccacccuu ggcaugaaca aaugcuacau ccaaauaaug      180

gaccuugguc acaugugcga cgcgaccaug agcuacgaau gccccaugcu agacgaagga      240

gucgaaccag acgacguaga cugcuggugc aacaccacaa gcaccugggu cguauacggc      300

accugccacc acaaaaaagg cgaagccaga cgcucacgac gagccgucac ucuucccucc      360

cacuccaccc gcaaacuaca aacgcgaucc caaaccuggu uagaaagccg cgaauacacc      420

aaacaccuua uaagggucga gaacuggaua uuccgcaauc ccggacuagc ucuugcagcc      480

gcagcaauag ccuggcuacu aggaucgucc accucacaaa aagucauaua ccuagucaug      540

auccuccuaa uagcuccagc cuacagcaua cguugcaucg gcguuuccaa ccgggacuuc      600

guagaaggca ugagcggcgg caccuggguc gacguuguac uggaacacgg aggaugcguc      660

accgucaugg cccaagacaa acccacuguc gacaucgaac uaguaaccac aaccgucagc      720

aacauggccg agguacgcag cuacugcuac gaggcgucca ucagcgacau ggcguccgac      780

ucacggugcc ccacccaagg cgaagcauac cuagacaaac aaucugacac ccaauacgua      840

ugcaaacgaa cccucgugga ccgaggaugg ggcaacggcu gcggccuguu cggcaaaggc      900

ucacuaguca ccugugcaaa auucgccugc uccaaaaaaa ugacagggaa auccauacaa      960

cccgaaaacc uagaauauag aaucaugcua agcgugcacg guucccagca uuccggcaug     1020

auaguaaacg acaccggcca cgaaaccgac gaaaaccgug caaaagucga aauaacucca     1080

aacucacccc gcgccgaagc aaccuuaggc ggcuucggcu cacucggccu agacugcgag     1140

ccccgcaccg gguuagacuu cuccgaccug uacuaccuua ccaugaacaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacaua ccccuaccau ggcacgcggg ggccgacaca     1260

ggaacccccc acuggaacaa uaaagaagcc cucgucgaau ucaaagacgc ccacgcuaag     1320

cgccaaaccg uagucguccu agggucacag gagggagccg uccacacugc ccuagccggc     1380

gcucucgaag cagaaaugga uggcgcaaaa gggcgacuua gcuccggaca ccuaaaaugu     1440

cgccucaaaa uggacaaauu gcgacuuaaa ggagucucuu acucucucug caccgccgcc     1500

uucacauuca caaaaauccc agccgaaacc cuccacggca ccgucaccgu agaaguccaa     1560

uaugcaggca ccgacggacc cugcaaaguc ccugcacaaa uggccgucga caugcaaaca     1620

cucacaccag uaggccgacu cauaacugcc aacccgguaa ucaccgaauc cacugaaaac     1680

ucgaaaauga ugcucgaacu cgacccacca uucggagacu cauacauagu cauagguguc     1740

ggagaaaaaa aaauaaccca ucacuggcac agaucaggcu caaccaucgg caaggcauuc     1800

gaagccaccg uaaggggcgc aaaacgcaug gccguccuag gagacaccgc augggacuuc     1860

ggaucgguag gcggcgcacu caacagccua ggcaaaggca uacaccagau auucggcgca     1920

gccuuuaaaa gccucuucgg cggaauguca ugguucagcc agauccucau aggcacacuc     1980

cuuauguggc uaggccuaaa cacaaaaaac ggauccaucu cccucaugug ccucgcccuc     2040

ggaggaguac ugaucuuccu aucgaccgca gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9545
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 prME-short mRNA Design 3 opt

<400>  9545
gggagaaagc uuaccaugac aagcguaggu auagucggcc uccuccucac caccgcaaug       60

gcagccgagg ucacacgcag aggcagcgca uacuacaugu accuagaccg gaacgacgca      120

ggagaagcaa ucucguuccc aaccacccuu gggaugaaca aaugcuacau acagauaaug      180

gaccugggac auaugugcga cgccacaaug uccuacgaau gcccaaugcu cgacgagggg      240

guagaacccg augacguaga cugcuggugc aacaccacaa gcacaugggu cgucuacggc      300

acaugccacc acaagaaagg ggaagcccgg aggagcagac gagcagucac acuccccagc      360

cacagcacaa gaaaacuaca gacccgcuca caaaccuggc uagaaagccg agaguacaca      420

aaacaccuca uacgaguuga aaacuggauc uuccgcaacc ccggauucgc gcuagccgcg      480

gcagcaauag cauggcuccu gggaucuagc accagucaaa aaguuaucua ccuugucaug      540

auacuccuaa uagcccccgc auacagcaua cgaugcauag gcguaagcaa ccgcgacuuc      600

gucgaaggga ugucaggagg gaccuggguc gacguaguac uagaacacgg cggaugcgug      660

acagucaugg cccaagacaa acccacagua gacauagagc ucgucacaac cacaguaucc      720

aacauggccg aaguccgcuc guacugcuac gaagcaucaa ucagcgacau ggccucagac      780

ucccgcugcc ccacgcaggg agaagcauac cucgacaaac aaucagacac acaguacguc      840

ugcaaaagga cccuaguaga ccgaggaugg gggaacgggu gcggacucuu uggaaaggga      900

ucgcucguaa caugcgcaaa auucgccugc agcaaaaaga ugacaggcaa aucaauacag      960

ccggaaaacc ucgaauaccg gaucaugcuc uccguacacg gcucacaaca cucgggcaug     1020

auagucaacg acacaggcca cgaaacagac gaaaacaggg ccaaagucga aaucaccccg     1080

aacucaccgc gcgcagaggc uacccuagga ggguucggau cccucggccu agacugcgaa     1140

ccgcgaacag gacuggacuu cagugaccuc uacuaccuaa ccaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacaua ccccuccccu ggcacgcagg agccgauacc     1260

ggcacgccgc acuggaacaa caaagaggcc cuagucgagu ucaaagacgc acacgcaaaa     1320

aggcaaacgg ucgucguacu cggcucgcaa gaaggagcag uacauacagc acuagcaggc     1380

gcacucgagg ccgaaaugga cggagccaaa gggcgccuuu ccagcggcca ccucaaaugc     1440

cggcuuaaaa uggacaaacu uaggcuaaaa ggggucuccu acagccucug caccgccgcc     1500

uucacauuca cuaaaauccc agcagagacc cuacacggaa cagugaccgu agaagugcaa     1560

uacgccggga cagacggacc cugcaaagua cccgcacaaa uggccguuga caugcagaca     1620

cuaacccccg ucggacgacu aaucaccgcc aauccgguga uaaccgaauc gacugaaaac     1680

agcaaaauga ugcucgaacu cgacccaccc uucggcgaca gcuacaucgu aauaggaguc     1740

ggcgaaaaga aaauaaccca ccacugguac cgcuccggaa gcaccaucgg caaagcguuc     1800

gaagccacgg ucagaggugc aaaacgcaug gccguccuag gcgauacggc cugggacuuc     1860

ggaagcguug ggggagcacu aaacucacua ggcaaaggga uacaccaaau auucggugcc     1920

gcauucaaau cccuguucgg aggaauguca ugguucucac aaauacuaau aggcacacua     1980

cuaauguggc ugggccucaa caccaagaac ggaagcauuu cccuaaugug ccucgcccuc     2040

ggcggcguac uaaucuuccu auccacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9546
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 prME-short mRNA Design 3 opt

<400>  9546
gggagaaagc uuaccaugac gucuguaggc auaguuggcc uacuccucac aacagccaug       60

gcagccgaag uaacccgccg cggcucagcc uauuacaugu accuggaccg aaacgacgcu      120

ggagaagcca ucucauuccc caccacccuc ggaaugaaca aaugcuacau acaaaucaug      180

gaccucggac acaugugcga cgcgaccaug aguuacgaau gcccaauguu ggacgaagga      240

gucgagcccg acgacguuga cugcuggugu aacaccacca guacuugggu cgucuacgga      300

accugccacc acaagaaagg ggaagcccga cgaucccgcc gggcgguaac ccuaccuucc      360

cacagcaccc gcaaacucca aacacgauca caaaccuggc ucgaaagcag agaauacacg      420

aaacaucuca uacgaguaga gaacuggaua uucagaaauc cgggauucgc acuagccgcc      480

gccgccaucg ccuggcuccu gggaucauca accagccaaa aaguaauaua ccucgugaug      540

auccuccuaa uagcccccgc guacucaaua cgcugcauag gcgucagcaa ccgggacuuc      600

guagaaggca uguccggggg caccuggguc gacguaguac ucgagcacgg cggcugcgug      660

accguaaugg cccaagacaa acccaccgua gacaucgaac ugguuacaac cacaguguca      720

aacauggcag aagucagaag cuacugcuac gaagccucga uaagcgacau ggccuccgac      780

agccgaugcc ccacccaagg agaagccuac cuagacaaac aaucagacac acaauacguc      840

ugcaaacgaa cccuaguaga ccgcggcugg ggcaacggau gcggccuuuu cggaaaaggc      900

ucacuuguaa ccugcgccaa auucgcaugc agcaagaaaa ugaccggaaa aucaauccaa      960

ccagaaaacc uagaguaucg caucaugcuc uccguacacg gaucacaaca cucaggcaug     1020

auagucaaug auacgggcca cgaaaccgac gaaaauagag caaaagucga gaucaccccc     1080

aacagccccc gcgccgaagc aacacucgga ggauucggga gccucggacu cgacugcgaa     1140

ccccgaacag gccuagacuu cucagaccua uacuaccuca ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ucacgacaua ccacuacccu ggcacgcugg ggccgacacc     1260

gggacucccc acuggaauaa caaagaagcc cuaguagagu ucaaagacgc ccacgccaaa     1320

cgccaaacgg ucguaguacu cggcagccaa gaaggggccg uccacaccgc gcucgccgga     1380

gcccucgaag ccgaaaugga cggagcaaag ggacggcuau ccucagggca ccucaaaugc     1440

agacuaaaga uggacaaacu ccgucugaag ggcguuuccu auagccucug caccgcagca     1500

uucaccuuca caaagauccc ggccgaaaca cuccacggga ccgucaccgu agaagugcaa     1560

uacgccggca cagauggccc cugcaaaguc cccgcccaga uggccgucga cacccaaacg     1620

cugacacccg ucggccgccu aaucaccgcg aaucccguca uaacugaauc caccgaaaac     1680

ucgaaaauga uguuagaacu cgauccccca uucggggacu cauacaucgu aauaggcguc     1740

ggagaaaaga aaauaaccca ccacuggcac cgaucaggca gcaccauagg caaagccuuc     1800

gaagccacug uaaggggcgc aaaacgcaug gcaguacuag gagacaccgc augggacuuu     1860

ggcucagucg gcggagcccu caacucccua ggcaaaggaa uacaccagau uuucggagca     1920

gcuuucaaaa gccucuucgg cggcaugagc ugguucuccc agauucuaau aggcacccug     1980

cucauguggc ucggacugaa caccaaaaac ggauccaucu cccucaugug ccuagcacug     2040

ggcggcgucc uaauauuccu gucuaccgcc guauccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9547
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 prME-short mRNA Design 3 opt

<400>  9547
gggagaaagc uuaccaugac auccgugggc auagucgguc uccuacuuac cacagcaaug       60

gcagccgaag uaacacgccg aggcucagca uacuacaugu accucgaccg gaacgacgca      120

ggcgaagcaa uauccuuccc aaccacacuc ggcaugaaca aaugcuacau ccaaaucaug      180

gaccucggcc acaugugcga cgccacaaug uccuacgaau gccccaugcu cgacgaaggg      240

guggaaccag acgacgucga cuguuggugc aacaccaccu ccaccugggu cgucuacggg      300

accugccacc acaagaaagg cgaagcccgc cgaagcagac gcgcagucac acucccuucc      360

cacucaaccc gcaaacugca aacccgcuca caaaccuggc ucgaaagccg cgaauacacc      420

aaacaccuua uaagaguaga aaacuggauc uuccgaaacc caggcuucgc acucgccgca      480

gccgccauag ccuggcuacu ugggagcucc acgucccaaa aaguaauaua ccucguaaug      540

auucuccuaa uagcgccagc cuacuccauc cggugcauag gagucucaaa cagagacuuc      600

gucgaaggua ugucgggagg aaccuggguc gacguaguac uagaacacgg gggcugcguc      660

acugucaugg cacaagacaa accgaccguc gacauagaac ucguaacaac caccgugucu      720

aacauggccg aaauccgaag cuacugcuac gaagcaagca uaagcgacau ggccuccgac      780

ucgcgaugcc caacccaagg agaagccuac cuugacaaac aaagugauac acaauacguc      840

ugcaagagaa cauuaguaga ccgaggaugg gggaacggau gcggccuauu cggcaaaggc      900

ucacucguca cgugcgccaa guucgcuugc uccaagaaaa ugaccggcaa aagcauacaa      960

ccugaaaacc ucgaauacag aauaaugcua uccguccacg gcucccaaca cagcggaaug     1020

aucgucaacg acacaggaca cgaaacagac gaaaaccgcg ccaaaguaga aaucaccccc     1080

aacucacccc gagcagaagc cacucucggc ggcuucggcu cccuaggacu agacugcgaa     1140

ccccgaaccg gccuggacuu uuccgaccuc uacuaccuga ccaugaauaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacaua cccuuacccu ggcacgccgg cgccgacacc     1260

ggcacacccc acuggaacaa caaagaagcc cuggucgagu ucaaggacgc acacgcaaaa     1320

cgucaaaccg uaguaguccu cggcucccaa gaaggcgccg uccacaccgc ccuagcaggg     1380

gcacuagaag cagaaaugga cggggccaaa ggaagacuau ccagcggcca ccucaaaugc     1440

cgacucaaaa uggacaaacu cagacucaaa ggaguaucau acagccuaug uaccgcagcg     1500

uucaccuuca ccaaaauccc agccgaaaca cuacacggga caguaaccgu agaaguacaa     1560

uacgccggaa cugacggacc cugcaaagua cccgcacaaa uggcugugga caugcaaacc     1620

uuaaccccag ugggcagacu gaucacagcc aaccccguaa uaacggaauc gacggaaaac     1680

uccaaaauga ugcucgaacu agacccuccc uucggcgacu cauacaucgu caucggcguc     1740

ggcgagaaaa agaucaccca ccauuggcac cgaagcggca gcacgauagg caaagcauuc     1800

gaagcaacag uaagaggcgc caaacgaaug gccguccuag gagacaccgc augggacuuc     1860

ggcagcguag gaggcgcacu aaacucacuc ggaaaaggaa uccaccaaau cuucggagca     1920

gccuucaaau cccuauucgg gggcaugagu ugguucuccc aaauacucau cggcacccua     1980

cuuauguggc ucggccuuaa caccaagaac ggaagcauaa gccugaugug ccucgcccug     2040

ggcggcgucc ucaucuuccu uucuaccgca guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9548
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 prME-short mRNA Design 3 opt

<400>  9548
gggagaaagc uuaccaugac cagcguggga aucgucggac uacuccuaac aaccgcgaug       60

gcugccgaag uaacccgacg cgggagcgcc uacuacaugu acuuagacag aaacgacgca      120

ggggaagcaa ucagcuuccc uacaacgcuc ggaaugaaca aaugcuacau acaaauaaug      180

gaccucggac acaugugcga cgcaacaaug aguuacgaau gcccaaugcu agacgaaggc      240

guagaaccag acgacguaga cugcuggugc aacaccacga gcacgugggu agucuacggu      300

acgugucacc acaagaaagg cgaagcacga cgcuccagga gagccguaac gcucccgagc      360

cacucgacac gaaaacucca aacacgcagc caaaccuggc uagaaucacg agaauacacg      420

aagcaccuaa uccgaguaga aaacuggaua uuccgaaacc ccggauucgc gcucgccgcc      480

gcggccaucg cauggcuccu ugggagcagc acaagccaaa aagugauaua ccuaguaaug      540

auccuacuca ucgccccagc auacaguaua cgaugcaucg gagugagcaa ccgcgacuuc      600

guggaaggaa uguccggggg caccuggguc gacgucgucc uggaacacgg cggaugcguc      660

acggucaugg cacaagacaa accaacagug gacauagaac ucgucaccac cacagucagc      720

aacauggcag aaguccgcag cuacugcuac gaagccagca ucucagacau ggccuccgac      780

agcagaugcc ccacccaagg cgaagcguac cuagacaaac aguccgacac gcaauacguc      840

ugcaaacgga cacucguaga cagaggaugg ggaaacggcu gcggccuguu cggcaaaggc      900

ucccuaguca ccugugcaaa guucgccugc agcaagaaaa ugacaggcaa aucaauccag      960

ccggaaaacc ucgaauacag aauaaugcuc agcguccacg gcucccaaca uagcggcaug     1020

auaguaaacg acacgggcca cgaaacagac gaaaaccgag ccaaagucga aauaacaccc     1080

aacagccccc gcgcggaagc cacacucgga ggcuucgggu cgcuaggacu cgacugugaa     1140

ccaagaacag gccuggacuu cagcgaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cuugugcaua aagaaugguu ccacgacaua ccacucccgu ggcaugccgg agcagacacc     1260

ggaacaccac acuggaacaa caaagaagcc cuaguggagu ucaaagacgc acacgcaaaa     1320

cgacaaacag uagucguacu aggaucccaa gaaggcgcug uacacaccgc acuagccggc     1380

gcacuagaag ccgaaaugga cggagcaaaa ggacgccuau ccucaggcca ccucaagugc     1440

aggcuaaaga uggacaaacu ccgauuaaag ggagucuccu acagccugug caccgccgcc     1500

uucaccuuca ccaaaauccc agccgaaaca cugcacggga ccgugacagu agaaguacaa     1560

uacgccggca cagauggacc cugcaaagua cuagcacaaa uggccgucga caugcaaaca     1620

cuaaccccag ucggccgccu cauaacggca aaccccguga ucacagaauc cacagaaaac     1680

ucgaaaauga ugcuagaacu cgacccaccc uucggcgacu cauacauagu aaucggagua     1740

ggagaaaaga aaauaaccca ccacuggcac agaagcggaa gcacaauagg caaagccuuc     1800

gaagcaaccg uccgaggagc caaaagaaug gcagucuuag gcgacaccgc augggacuuc     1860

gggagcgugg gcggagcacu aaacagcuug ggaaaaggaa uccaccaaau auucggagca     1920

gcguucaaaa gccuguucgg aggcaugagu ugguucaguc agauccucau cggcacacuc     1980

cucauguggc uaggacuaaa caccaaaaac ggcagcaucu cacugaugug ccucgcgcuc     2040

ggaggcguac ugauauuccu gagcaccgcc guauccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9549
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX446950.2 prME-short mRNA Design 3 opt

<400>  9549
gggagaaagc uuaccaugac cucagucggg auugucggac uccuacucac cacagccaug       60

gcagccgagg ugacccgacg aggaucagcc uacuacaugu aucucgaucg caacgacgca      120

ggcgaagcca uaucguuccc cacaacccuc ggcaugaaca aaugcuacau ccaaaucaug      180

gacuuaggcc auaugugcga cgccaccaug ucauacgaau gccccacacu agacgaaggc      240

gucgaacccg acgacgucga cugcuggugc aauaccaccu ccacaugggu cguauacggc      300

acaugccacc acaagaaagg cgaagcccgc cgaucccgca gagccguuac cuuaccaagc      360

cacuccaccc gaaaacucca aacgcggagc caaaccuggc uagaaagccg cgaauacaca      420

aaacaccuca uccgcgucga aaacuggaua uuccgcaacc caggauucgc ccucgccgcc      480

gccgcaauag cauggcugcu cggcuccagc acaucccaaa aagucaucua ccucguaaug      540

auccuucuca ucgcccccgc cuacuccauc cgcugcaucg gcgucucaaa ccgagacuuc      600

gucgaaggca ugucaggcgg caccuggguc gacgucgucc uggaacacgg aggcugcgua      660

accgucaugg cccaagacaa accgacagua gacaucgagc uagugaccac aacagucagc      720

aauauggccg aagucagauc uuacugcuau gaagcgucca ucucugacau ggccuccgac      780

ucacgcuguc ccacgcaagg ggaagccuac cuggacaaac aaagcgacac gcaguacguc      840

uguaaacgca cccuagucga ccgcggcugg ggcaacggau gcggacuauu uggaaaaggc      900

ucacucguca cuugcgccaa auucgccugc ucaaagaaga ugaccggaaa gucuauccaa      960

ccggaaaacc uagaauaccg cauaaugcua uccguccacg ggucacaaca cucaggaaug     1020

auagucaaug acaccggaca cgaaaccgac gaaaaccgug cuaaaguaga aaucaccccc     1080

aacuccccua gagccgaagc aacccuaggg ggauucggaa gucuaggccu ggacugcgaa     1140

ccacgcaccg gccuggacuu cuccgaccuc uacuaccuca caaugaacaa caagcacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccacuaccuu ggcacgccgg cgccgacaca     1260

ggcacacccc acuggaacaa caaagaagcc cucguagagu ucaaagacgc ucacgccaaa     1320

cgucaaaccg ucgucguccu agggagccaa gaaggcgcag uccacaccgc guuggcaggc     1380

gcccuagaag cagaaaugga cggagcaaaa ggacgacucu ccucgggcca ccuaaaaugc     1440

cggcuaaaaa uggacaaacu acgccucaaa ggcgucuccu acagccucug caccgcagcc     1500

uucaccuuca ccaagauccc agccgaaacc cuucacggca ccguaaccgu agaaguccaa     1560

uacgcaggca cagacggccc augcaaagua ccagcccaaa uggcaguaga uaugcaaaca     1620

cuaaccccug uuggccgccu caucacagcc aaccccguaa ucaccgaaag caccgagaac     1680

ucaaaaauga ugcuagagcu cgacccaccc uuuggcgauu ccuacaucgu caucggggua     1740

ggcgaaaaga aaaucacgca ccacuggcac agguccggaa gcacaaucgg caaagcguuc     1800

gaggcaacug uccgaggcgc caaacgaaug gcaguccucg gugacaccgc cugggauuuc     1860

ggaagcgucg gcggugcccu caauagccua ggcaagggca uccaccaaau cuucggcgcc     1920

gccuucaaau cccuguucgg aggcauguca ugguucucac agauccuaau aggcacccug     1980

cuaauguggc uaggccucaa caccaaaaac ggaucgaucu ccuuaaugug ccuagcacuc     2040

ggcggggucc uaaucuuccu auccacagcc guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9550
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003157.1 prME-short mRNA Design 3 opt

<400>  9550
gggagaaagc uuaccaugac cucgguaggg aucgucggcc uacuccuaac aacagccaug       60

gccgccgaag ucacccgccg aggaagugcc uacuacaugu acuuagaccg aaacgacgcc      120

ggggaggcca uaucguuccc cacaacacua ggaaugaaca aaugcuacau ucagauaaug      180

gaccuaggac acauguguga cgcaaccaug aguuacgaau gucccaugcu ggacgaagga      240

guagagcccg acgacguaga cugcuggugc aacacaacca gcacuugggu agucuacgga      300

acgugucauc acaagaaagg cgaagcacga cgaucccgaa gggcuguaac gcugccauca      360

cacucgaccc gaaaacuuca aacacgaucg caaacauggc uagagucccg cgaauacaca      420

aaacaccuca uacgcgucga aaacuggaua uuccgcaacc ccggcuucgc ccuggccgca      480

gccgccaucg cauggcuccu cggcagcagu accucccaaa aagucaucua ccucguaaug      540

auccuacuaa ucgccccagc auacagcaua cgaugcaucg gaguaucaaa ccgcgacuuc      600

gucgaaggca ugagcggcgg aacaugggua gacguaguau uagaacacgg aggcugcgua      660

acuguaaugg cgcaagacaa accgacagua gacaucgagc uagucaccac caccgucagu      720

aacauggcag aaguccgguc auacugcuac gaagccagca uaucagacau ggcguccgac      780

agccggugcc ccacccaggg cgaagccuac cuagacaaac aauccgacac ccaauacgug      840

ugcaaaagaa cccuaguaga cagaggaugg ggcaacggau gcggccuauu cgggaaaggg      900

ucgcucguaa caugcgcaaa auucgccugc ucaaagaaaa ugacagggaa aucaauccaa      960

ccagaaaacc uagaauacag aaucaugcua ucuguccacg gcucccagca cagcggcaug     1020

auagucaacg acacaggcca cgaaaccgac gaaaacaggg ccaaaguaga aaucacacca     1080

aacuccccac gcgccgaagc aacauuaggc ggcuucggca gccuaggccu cgacugcgag     1140

ccccgaacgg gccuugacuu cuccgaccua uacuaccuca ccaugaacaa caaacacugg     1200

cuuguacaca aagagugguu ccacgacaua ccccuacccu ggcacgcagg cgccgacaca     1260

ggcacacccc acuggaacaa caaagaagcc cuggucgagu ucaaagacgc acacgcaaaa     1320

cgccaaacag uaguuguacu cggaagccag gaaggcgcug uacacacagc ccucgcagga     1380

gcucuggaag ccgaaaugga cggugcaaaa ggccgacucu caagcggcca ccugaaaugc     1440

agacuaaaaa uggacaaacu cagacucaaa ggggugagcu acagccucug cacagccgca     1500

uucacauuca caaaaauacc agccgaaacc cuucacggua cagucacagu ggagguccaa     1560

uacgcaggaa cggacggacc augcaaagug cccgcccaaa uggcagucga caugcaaacc     1620

cucacgcccg uagggaggcu cauaacagcu aaccccguaa uaacagagag cacggaaaac     1680

agcaaaauga ugcucgagcu cgacccgccc uuuggggaca gcuacauagu caucggcgua     1740

ggagaaaaga aaaucacaca ucacuggcac cgaagcggcu caacaauagg caaagcguuc     1800

gaagcaacag uacgaggcgc caaaagaaug gcugugcucg gagacaccgc cugggacuuc     1860

gguuccguag gcggagcacu caacucucug ggcaaaggca uacaccaaau cuucggagca     1920

gccuucaaau cccucuucgg aggaauguca ugguucagcc aaauccucau aggaacacug     1980

cucauguggc ucggacuaaa caccaaaaac ggcagcauau cccuaaugug ccuggcacua     2040

ggaggcgucc uaauauuccu cucuaccgug guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9551
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX811222.1 prME-short mRNA Design 3 opt

<400>  9551
gggagaaagc uuaccaugac cagcguaggc aucgucgggc uacuacuaac aaccgcgaug       60

gccgccgaag ucacacgcag aggguccgcc uauuacaugu accuagacag gaacgacgcc      120

ggcgaagcaa uaucauuccc gaccacgcug gggaugaaca aaugcuacau acagauaaug      180

gaccucgggc acaugugcga cgcaacaaug aguuacgaau gccccaugcu ggacgaaggc      240

guagaaccgg acgacgucga cugcuggugc aacacaacga gugcaugggu ggucuacgga      300

accugccacc acaagaaagg cgaagcgcgc agaagccgcc gagcagucac acuaccaagc      360

cacaguacac guaagcucca aacaagacca caaacauggc ucgagucccg agaguacaca      420

aaacacuuaa uacgaguaga gaacuggauc uucagaaacc caggauucgc acucgcagcc      480

gcagcaaucg cauggcuacu ggguuccagc accagccaaa aagucaucua ccucguaaug      540

auacuccuaa ucgccccggc cuacuccauc aggugcaucg gaguaagcaa ccgcgauuuc      600

gucgaaggga ugucgggagg aaccugggua gacgucguac ucgaacacgg cggcuguguc      660

acgguaaugg cccaagacaa accaacugua gacaucgagc uaguaaccac gaccguauca      720

aacauggccg aagucagaag cuacugcuac gaagcaucaa ucagcgacau ggccuccgac      780

agcagaugcc ccacccaagg cgaagccuac cuggacaaac aaagcgacac acaauacgua      840

ugcaagagaa cacuggucga ccgaggaugg ggcaacggcu gcggccucuu cgguaaaggg      900

ucgcuaguua caugcgccaa auucgcgugc agcaagaaaa ugaccggaaa aagcauccaa      960

ccagaaaacc ucgaauacag aaucaugcua uccguacacg gcagccagca cagcggcaug     1020

auaguaaacg acaccgggca cgaaacagac gaaaacaggg caaaagucga gauaacaccc     1080

aacucuccac gagccgaagc aacccucgga ggauucggga gccucggacu agacugugaa     1140

ccgaggaccg gccuagacuu cagcgaccug uacuacuuaa caaugaauaa caaacacugg     1200

cuuguacaca aagagugguu ccacgacauc ccccuaccau ggcacgccgg cgcagacacc     1260

ggaacgccgc acuggaacaa uaaagaggcc cuaguagagu ucaaagacgc ccacgcaaaa     1320

cgacaaacug uagucguccu aggcucacaa gagggagcag uccauaccgc ccuagccgga     1380

gcgcucgaag cagagaugga cggagcgaaa gggcgccuau caagcggaca ccuaaagugc     1440

aggcuaaaaa uggauaaacu gcgccucaag ggcgucagcu acucacucug cacagcagca     1500

uucacauuca caaaaauccc ggccgaaacc cuacacggaa ccguaacagu agaaguacag     1560

uacgcaggaa ccgacggacc cugcaaaguc ccggcccaaa uggcgguuga caugcaaacc     1620

cuaacgcccg uaggacgccu gaucacagcc aaccccguaa ucacagaauc aaccgaaaau     1680

ucaaagauga ugcuggaacu ggacccgcca uucggagaca gcuacaucgu aauagggguc     1740

ggcgaaaaga aaauaacaca ccacuggcac cgauccggaa gcaccauugg aaaagcauuc     1800

gaggccacag ucaggggagc gaaacgaaug gcaguccucg gggauacagc augggacuuc     1860

ggauccguag gcggagcccu gaacucacug ggcaaaggaa uccaccaaau auucggagca     1920

gcguucaaau cacuauucgg aggcaugagc ugguucucac aaauacuaau aggcacacua     1980

cuaauguggc ucggccuaaa cacgaaaaac ggcagcaucu cccuaaugug ccuggcccua     2040

ggaggcguac uaauauuccu aucaacagcc guuuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9552
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 prME-short mRNA Design 3 opt

<400>  9552
gggagaaagc uuaccaugac aucagucgga aucgucggcc ucuugcuuac aacagccaug       60

gccgcggagg uaacacgccg gggcagcgcc uacuacaugu accuagacag aaacgacgcc      120

ggcgaagcaa ucagcuuccc caccacacuc gguaugaaca aaugcuacau ccaaaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgaau gccccaugcu agacgaaggc      240

gucgaacccg acgacguaga cugcuggugc aacaccacca gcacaugggu ggucuacgga      300

accugccacc acaagaaagg agaagcccga aggagccgcc gcgcagucac ccuacccagc      360

cacucaacaa gaaaacugca gacacgaagc caaacauggc uagaaucccg cgaauacaca      420

aaacaccuaa ucagagucga aaacuggauc uucagaaauc caggcuucgc acucgccgcu      480

gcagcaauag cguggcuacu cggcagcuca acaucaaaga aaguaaucua ccuaguaaug      540

auccuacuga uagcaccagc cuacagcauc agaugcaucg ggguauccaa cagagacuuc      600

gucgaaggca ugagcggagg gacgugggua gacgucgucc ucgaacacgg cggaugcguc      660

accguaaugg cacaagacaa accaacaguc gacauagaac ucguaaccac aaccgucucg      720

aacauggccg aaguccgaag cuacugcuac gaagcgagca ucagcgacau ggcaucagac      780

ucacggugcc caacccaagg agaagcauac cuagacaaac agagcgacac ccaauacguc      840

uguaaaagaa cccucgucga ccgcggaugg ggcaacggcu gcggccuauu cggaaaagga      900

ucacucguaa ccugcgccaa auucgccugc uccaagaaaa ugacaggaaa aaguauucag      960

cccgaaaacc uugaguaccg caucaugcug ucgguccacg gaagucaaca cagcggcaug     1020

aucguaaacg acacuggaca cgagaccgac gaaaaucgag caaaagucga aauaacaccg     1080

aacucacccc gcgccgaagc cacccuuggc ggauucggau cccucggccu agacugcgaa     1140

cccagaaccg gccuagacuu cucggaccuc uauuaucuca cgaugaacaa caaacacugg     1200

cuuguccaca aagaaugguu ccacgacauu ccacuacccu ggcacgcagg agcagacacc     1260

ggcaccccac acuggaacaa caaagaagcc cuaguggagu ucaaagacgc ccacgccaaa     1320

cgccagacag uaguaguccu aggcucccaa gaaggagccg uccacacagc ccuggccggc     1380

gcacuagaag ccgaaaugga cggggcgaaa gggcgccuua guagcgggca ucuuaaaugc     1440

cgccuaaaaa uggacaaacu cagacucaaa ggagucagcu auucccucug cacugcagca     1500

uucaccuuca cuaaaauccc agccgaaacc cuacacggca ccguaaccgu ggaaguacaa     1560

uacgcgggca cagacggccc augcaaagua cccgcccaaa uggccguaga caugcaaacc     1620

cucacaccag ugggcaggcu cauaacagcc aaccccguca ucacggaauc gaccgagaac     1680

aguaaaauga ugcuggaauu agacccgccc uucggagaua gcuacaucgu aaucggagua     1740

ggagagaaga aaaucacaca ccacuggcac cgaucaggca gcacaaucgg caaggcuuuc     1800

gaggccaccg uaagaggcgc gaaacgcaug gcaguacuag gcgacacagc cugggacuuc     1860

ggcucaguug gaggcgcccu aaacagccua ggcaaaggca uccaccaaau cuucggcgcc     1920

gcauuuaaaa gccuauucgg cggaaugucc ugguucagcc agauccugau cggaacucuc     1980

cuaauguggc ugggguuaaa caccaaaaac gggucgaucu cacuaaugug ccucgcacua     2040

ggcggaguac uaaucuuccu gagcacagca gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9553
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 prME-short mRNA Design 3 opt

<400>  9553
gggagaaagc uuaccaugac auccgucggg aucgucggcc uccuacugac aaccgcuaug       60

gccgccgaag ucacacgcag aggauccgcu uauuacaugu accucgaccg aaacgacgca      120

ggcgaagcga ucaguuuccc cacaacccuc ggaaugaaca aaugcuacau acaaaucaug      180

gaccucggac acaugugcga ugcgacgaug uccuacgaau gccccauguu agacgaaggc      240

gucgaacccg acgauguaga cugcuggugc aacacaacca gcaccugggu aguauacggc      300

acaugccacc acaagaaagg agaggcccgc agaucacgca gagcagucac acuacccagu      360

cacagcacga gaaaacucca aacaaggagc cagacauggc ucgagagccg ggaauacaca      420

aaacaccuaa uacgaguaga aaacuggauc uuccgaaacc caggauucgc gcucgccgcc      480

gccgccaucg ccuggcuccu cggcagcagc accagacaga aaguaauaua ccucgucaug      540

auccuccuaa uagcaccagc auauucaaua cgaugcaucg ggguaagcaa ccgcgacuuc      600

guugaaggga uguccggcgg aacaugggua gaugucgucc uugaacacgg cggcugcgua      660

accguaaugg cccaagacaa acccacggua gacauagagc ucguuacaac aaccguaucc      720

aacauggccg aagucaggag cuacugcuac gaggccagca uauccgacau ggccagcgac      780

agcagauguc ccacacaagg agaagcguac cucgacaaac aaagcgacac acaauacgua      840

ugcaaacgaa cccuagucga cagaggcugg ggaaacggau gcggccucuu cggcaagggc      900

ucccucguaa ccugugcaaa guucgccugc uccaagaaga ugaccggcaa aagcauacaa      960

cccgaaaacc uagaauaucg aaucaugcua agcguacacg ggagccaaca cucaggcaug     1020

aucgucaaug acacaggcca cgaaacagac gaaaacaggg ccaagguaga aaucaccccc     1080

aacagccccc gcgccgaagc cacccugggc ggauucggca gccuaggccu agacugcgaa     1140

cccagaacag gacuagacuu cuccgaccuc uacuaccuca caaugaacaa uaaacauugg     1200

cugguacaca aggaaugguu ccacgacaua ccccucccuu ggcacgccgg agcagacacc     1260

ggaacuccac acuggaacaa caaagaagcc cuaguggagu ucaaggacgc acacgcaaag     1320

agacaaacgg ucguaguacu gggaucacaa gaaggcgcag uccacaccgc ccucgcggga     1380

gcacuagaag ccgaaaugga cggcgcaaaa ggccgacuau ccagcgggca ccuaaaaugc     1440

agacuaaaaa uggacaaacu ccgacuaaaa ggagucaguu acagccuaug caccgccgca     1500

uucacguuca caaaaauccc ggccgagacg cugcacggca ccgucacggu ugaagugcaa     1560

uacgccggca cagacggccc cugcaagguu cccgcccaaa uggccguaga caugcaaacg     1620

cuaaccccag ucggccgccu uauaacggcc aaccccguaa ucaccgaauc aacagaaaac     1680

uccaaaauga ugcucgaacu agacccacca uucggcgaca gcuacaucgu aaucggcguc     1740

ggcgaaaaga aaauaacgca ccacuggcau cgcagcggaa gcacaaucgg caaagcguuc     1800

gaagccaccg uacgcggagc caaacgcaug gccgugcucg gcgacacagc cugggacuuc     1860

ggaucagucg gcggagcccu aaacagccuc ggcaaaggca uacaccaaau auuuggagcg     1920

gccuucaaau cccuauucgg agggaugagc ugguucuccc aaauccugau cggaacccuc     1980

cucauguggc ucggacucaa cacgaaaaac ggcagcauaa gccuaaugug ccuggccuug     2040

ggcgguguac ucauauuucu aagcaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9554
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 prME-short mRNA Design 3 opt

<400>  9554
gggagaaagc uuaccaugac caguguaggg auaguaggcc uacuccucac aacggccaug       60

gcagcagaag ucacccguag aggaagcgcc uauuacaugu accuagaccg aaacgaugcg      120

ggcgaggcca ucagcuuccc aaccacucua ggcaugaaua aaugcuacau acaaaucaug      180

gaccucggac auaugugcga cgcgacaaug aguuacgaau gccccaugcu agacgagggc      240

guagaaccag acgacgugga cugcuggugc aauacaaccu ccacaugggu cgucuacgga      300

acaugccacc acaagaaagg cgaggcccga cgcucgcgcc gcgcuguaac auuacccucg      360

cacuccaccc gaaaacucca aacacggagc caaacguggc ucgaaagcag agaauacaca      420

aaacacuuaa uaagaguaga aaacuggaua uuucgaaacc ccggcuucgc ccuagccgcg      480

gcagcgaucg cauggcuccu uggaagcagc acaagccaga aagucauaua ccuagugaug      540

auccuccuca ucgcgcccgc auacucaauc cggugcauag gcgucagcaa cagagacuuc      600

guagaaggaa ugagcggcgg caccuggguc gacguagugc ucgagcacgg cggaugcguc      660

accguaaugg cacaggacaa accaaccguc gacaucgaac ucgucacaac cacggucuca      720

aacauggccg aagucagauc guacugcuac gaggccucca ucagcgacau ggccagugac      780

uccagaugcc ccacgcaagg ggaagccuac cucgacaaac aaucagacac acaauacgua      840

ugcaaacgaa cacucgucga caggggaugg ggaaacggcu gcggccucuu cgguaaagga      900

ucgcugguaa ccugcgccaa auucgccugc agcaagaaaa ugaccgggaa aagcauccaa      960

ccugaaaacc uagaauaccg cauaaugcuc ucaguccacg ggucccagca cagcggcaug     1020

auagucaacg acacaggcca cgaaaccgac gaaaacaggg ccaaaguaga aaucaccccc     1080

aacagcccac gcgccgaagc aacgcuaggc ggcuuuggau cccucggacu agacugugaa     1140

ccacgaaccg gccucgacuu cuccgaccua uacuaucuca ccaugaauaa caagcacugg     1200

cucgugcaca aagaaugguu ccacgacauc ccacucccau ggcacgccgg cgccgacacc     1260

ggaacgccac auuggaacaa caaagaagcc cuaguggagu ucaaagacgc acacgcaaaa     1320

aggcaaacag ucgucguacu cggaacucaa gaaggagcag uccacacagc gcucgcagga     1380

gcacucgaag cagaaaugga cggggcaaag ggcagacuau ccagcggaca ccucaagugc     1440

cggcucaaga uggacaaacu acgccuaaaa ggcguaagcu acagccuaug caccgcagcc     1500

uucaccuuca ccaagauacc cgcagaaacc cuacacggca cagucacagu ggaaguccaa     1560

uacgccggca ccgacggacc cugcaaaguc cccgcccaaa uggcagucga caugcagacg     1620

cucacacccg uaggacguuu aaucacagcg aaccccguaa uaaccgaguc aaccgaaaac     1680

agcaaaauga ugcuagaacu cgacccacca uucggcgaca guuacauagu caucggcgua     1740

ggcgagaaaa agauaaccca ccacuggcac cgaagcggga gcacaauagg caaagccuuc     1800

gaagcaaccg uccgaggagc caaaagaaug gccguccuag gagacaccgc cugggauuuc     1860

gggagcgucg gcggggcacu caacagccuc ggaaaaggca uacaccaaau auucggggca     1920

gcauucaaga gcuuguuugg agggauguca ugguucuccc agauccucau cggcacccua     1980

cucauguggc uaggacuaaa caccaaaaac gggucgaucu cacucaugug ccuagcccuc     2040

gggggcguac uuaucuuccu aucaacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9555
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 prME-short mRNA Design 3 opt

<400>  9555
gggagaaagc uuaccaugac gagcgucggc aucguaggac uacuccucac uaccgccaug       60

gcagccgaag ucacaagacg aggauccgcc uacuacaugu accuagaccg caacgacgca      120

ggcgaagcaa uauccuuccc aaccacgcug gguaugaaca aaugcuacau acaaauaaug      180

gaccucggac acaugugcga cgcaacaaug ucguacgaau gcccgaugcu agacgaagga      240

guagagccag acgacgucga cugcuggugc aacacaaccu ccacgugggu cguguacggc      300

acaugccacc acaaaaaggg agaggcccga agaagccgac gagccguaac ccuccccuca      360

cacucgaccc gcaaacuuca gacacgcagc caaacauggc uagaaagcag agaauacacc      420

aagcaccuga ucaggguaga aaauuggaua uuccgcaacc ccggauuugc ccuagcagcc      480

gcagccaucg cauggcuccu gggauccagc acaucacaga aaguaauaua ccuuguaaug      540

auccuacuaa ucgcaccagc cuacucccuc cggugcauag gcgucagcaa ccgcgacuuc      600

gucgaaggca ugucaggcgg gaccugggua gacguaguac ucgaacaugg gggaugcguc      660

accguaaugg cacaggacaa accaaccguc gacaucgagc ugguaaccac aacaguaagc      720

aacauggcag aagugcgcuc auacugcuac gaagcaucca ucagcgacau ggccagcgac      780

ucccgcugcc caacacaagg cgaagccuac cuagacaaac aaagcgacac ccaauacguc      840

ugcaagagaa cccucguaga ccgaggcugg gguaacggcu gcggacucuu cggcaaaggc      900

ucacucguga cuugcgccaa guucgcaugc agcaagaaaa ugacaggcaa aagcauacaa      960

ccagaaaacc uagaauacag aaucaugcuc agcguucacg gaucccaaca cuccgggaug     1020

aucgucaacg acacaggcca cgaaacagac gaaaaccgcg caaaagucga aaucacacca     1080

aacagcccac gcgcagaagc aacacuuggc ggauucggca gccucgggcu agacugcgaa     1140

ccccgaacgg gccuggacuu cagcgaccua uacuaccuaa ccaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccccucccau ggcacgccgg agccgacaca     1260

ggaacacccc acuggaacaa uaaagaagcc cuaguagagu ucaaagacgc ccacgccaaa     1320

cgccaaacag uaguaguucu agggagccaa gaaggagcag uccacacagc gcucgcaggc     1380

gcccuggagg cagaaaugga cggagccaaa ggacggcucu ccagcggaca ccuuaagugc     1440

cggcuaaaaa uggacaaacu cagacucaaa ggcguaucuu acaguuuaug uaccgccgcc     1500

uuuacauuca ccaagauccc cgcugaaaca cuucacggga ccgucacagu cgaaguccaa     1560

uacgcgggua ccgacggacc augcaagguc cccgcgcaaa uggcaguaga caugcagaca     1620

uuaacacccg uaggccggcu uaucacagca aacccaguaa ucacagaauc aacagaaaac     1680

ucaaaaauga ugcucgagcu agacccgccc uucggggacu cauacaucgu caucggaguc     1740

ggagagaaaa agaucacaca ccacuggcac cgcagcggca gcacgaucgg aaaagcguuc     1800

gaagccacug ugcgcggagc gaaaagaaug gcaguacugg gagacacugc gugggacuuc     1860

ggcucagucg gaggggcacu aaacagccug ggcaaaggaa uacaccaaau auucggcgcg     1920

gcauucaaau cccucuucgg aggaaugagu ugguucucac aaauccugau cggcacccua     1980

cucauguggc ugggucucaa caccaaaaac gggucaauaa gccucaugug ccucgcacua     2040

ggcggaguac uaaucuuccu caguacagca guaagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9556
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075932.1 prME-short mRNA Design 3 opt

<400>  9556
gggagaaagc uuaccaugac cuccgucggg auaguaggcc uacuacucac caccgcuaug       60

gcagcagaag uaacccgaag gggcagcgca uauuacaugu accuagacag aaacgacgcc      120

ggcgaagcga ucaguuuccc gacaacgcug ggaaugaaca aaugcuacau acaaaucaug      180

gaccucggac acauguguga cgccaccaug ucauacgaau gcccgaugcu cgacgaggga      240

gucgaacccg augauguaga cugcuggugc aacacaacaa gcacuugggu cgucuacggg      300

accugccacc acaagaaagg cgaagcacgu cgcagccgca gagcuguaac ccucccaucg      360

cacagcacac gcaaacucca aacccgcucc caaacguggc uggaaucccg cgaguacacc      420

aaacacuuaa ucagaaucga aaauuggauc uuccgcaacc ccggcuucgc acucgcggca      480

gccgccauag cguggcuccu aggcucaucc acgagucaaa aaguaauaua ccucgucaug      540

auccuacuaa uagcccccgc cuacuccaua cgcugcauag gcgucagcaa ccgagacuuc      600

guagaaggaa ugucuggagg caccuggguc gacguaguac uggaacacgg cggcugcguc      660

acggugaugg cacaagacaa accgaccgua gacaucgaac ucgucaccac cacaguaagc      720

aacauggcug agguccgcuc guacugcuac gaggcaagca uaagcgacau ggcaucagac      780

uccaggugcc ccacccaagg agaagccuac cucgacaaac aaagcgacac ccaauaugua      840

ugcaaaagga cccuagucga ccgcgggugg ggaaacggcu gcggacuauu cggcaaagga      900

agccucguua ccugcgcaaa auucgcaugc uccaagaaaa ugaccggcaa aucuauacaa      960

ccagaaaacc ucgaauacag aauaaugcua ucaguccacg gcucacaaca cagcggcaug     1020

aucgucaaug acaccggcca cgaaaccgac gaaaacagag caaaaguaga aaucaccccc     1080

aacagcccac gagccgaggc cacccucggc ggcuucggau cgcuggggcu agacugcgaa     1140

cccagaacag gccucgacuu cucugaccua uacuaccuaa cgaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacauc ccacuaccau ggcacgcagg cgcagacaca     1260

ggaacacccc acuggaauaa uaaagaggca cuugucgagu ucaaagacgc acacgccaaa     1320

agacaaaccg ucguaguccu cggcagccaa gaaggugcug uacacaccgc acucgccggc     1380

gcccucgaag cagaaaugga cggcgcaaaa ggccgccuau caucaggcca ccuaaaaugc     1440

cgccucaaaa uggacaaacu ccgacuaaaa ggcgucagcu acucgcucug caccgcggcc     1500

uucaccuuca ccaaaauccc cgcagaaacc cuacacggaa ccgugaccgu cgaaguccaa     1560

uacgcaggaa ccgacggacc augcaaaguc ccagcacaaa uggccgucga caugcagaca     1620

cuaacaccag uaggcagacu cauaacugcc aacccaguaa ucacagaauc caccgaaaac     1680

aguaaaauga ugcucgaacu agacccgcca uucggcgaca gcuacaucgu caucggcguc     1740

ggggaaaaga aaaucacaca ucacuggcac cgcucaggau ccacaaucgg caaagccuuc     1800

gaagccacag uccgaggggc uaaaagaaug gcaguccucg gcgacaccgc augggacuuc     1860

ggcagcguag gcggcgcccu aaacucacuc ggaaaaggca uucaccaaau auucggcgca     1920

gcauucaaau cacuuuucgg aggaaugagc ugguucucac aaauacuaau cggaacacua     1980

cuaauguggc ucggacucaa caccaaaaac ggguccauaa gccugaugug ucuagcccua     2040

ggaggaguac ucaucuuccu aagcaccgca gucuccgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9557
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 prME-short mRNA Design 3 opt

<400>  9557
gggagaaagc uuaccaugac aucaguggga auaguaggac uccuacuaac gaccgcaaug       60

gccgcagaag uuacccgaag aggcagcgca uacuauaugu accucgaccg caacgacgcc      120

ggcgaagcca ucucauuccc aaccacacuc gggaugaaca aaugcuacau ccaaauaaug      180

gaccuaggac acaugugcga cgcgaccaug ucauacgagu gucccaugcu ugacgaaggc      240

guagaaccgg acgacguaga cugcuggugc aacaccacga gcaccugggu gguauacggc      300

accugccacc acaaaaaagg cgaggcgaga aggagcagac gagccgucac ccuucccagc      360

cauucaaccc gaaaacuaca aacaagaagu caaacguggc uagaaucacg cgaauacaca      420

aaacaccuga uccgcguaga aaacuggauc cuaaggaacc caggauucgc acuggccgcc      480

gcggccaucg ccuggcuccu agggagcagc acaucccaaa aaguaaucua ccuagucaug      540

auccuccuga uagcgcccgc guacagcauc agauguaucg gcgucuccaa ccgagacuuc      600

guagaaggaa ugucuggggg uacauggguc gacgucgucc uagaacacgg cggcugcguc      660

acaguaaugg cacaagacaa accaacggua gacauagaac ucgugaccac caccgucuca      720

aacauggcag aaguacgauc guacugcuac gaggcgucca uaucagacau ggccagcgac      780

agccgcugcc caacccaggg agaggcauac cucgacaaac aauccgacac ccaauacgug      840

ugcaaaagga cacuagucga ccgggggugg gggaacggcu gcggccucuu uggaaaaggc      900

agccucguca caugcgccaa auucgccugc uccaaaaaaa ugacagggaa aagcauccaa      960

ccagaaaacc uagaauaccg aaucaugcuc uccgugcacg gcucacaaca cuccgggaug     1020

auaguaaacg acacaggaca cgagaccgac gaaaaccgag ccaaaguuga aauaaccccc     1080

aacagcccca gagcugaagc cacacuugga ggauucggau cccuaggccu cgacugcgaa     1140

ccacgcacgg gccucgacuu uagcgaccua uacuaccuaa ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacauc ccgcugccau ggcacgccgg cgccgacacg     1260

ggaacccccc acuggaacaa caaagaggca cucgucgaau uuaaagacgc acacgccaaa     1320

cgacaaaccg ucguuguacu aggcucacaa gagggcgcgg uccauacagc acuagcaggc     1380

gcccuggagg ccgaaaugga cggagccaaa ggccgacucu ccagcggaca ucuaaaaugc     1440

cgacugaaga uggauaaacu acgacucaaa ggagucucau acucacuaug uaccgcagca     1500

uuuacauuca caaaaauacc cgcagaaaca cuacacggaa ccguaaccgu cgaaguccag     1560

uacgccggca cggacggccc cugcaaaguc cccgcccaaa uggccgucga caugcaaaca     1620

cugaccccag ucggccgccu cauaaccgca aacccaguca ucacugaauc caccgagaac     1680

agcaaaauga ugcucgagcu cgaccccccc uucggcgaca gcuacaucgu caucggcauc     1740

ggggaaaaga aaauaacaca ccacuggcac cgauccggca gcaccaucgg caaagccuuc     1800

gaggcaacag uccgcggagc aaaacgcaug gcaguccucg gagacaccgc cugggacuuc     1860

ggcagcgucg gaggagcccu caacucucug ggcaaaggga uacaccaaau cuucggagca     1920

gcauucaaaa gccuauucgg cggaaugagc ugguucuccc aaauacucau aggaacccuc     1980

cucauguggc ucggacuaaa caccaaaaac ggauccauaa gccuaaugug ccuagcucuc     2040

ggaggcgugc uaauauucuu aaguaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9558
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 prME-short mRNA Design 3 opt

<400>  9558
gggagaaagc uuaccaugac cuccguagga aucgucggcc uccugcuaac caccgccaug       60

gcagccgaag ucacacgccg cggcucagca uacuacaugu aucucgacag aaaugacgcc      120

ggugaagcca uaucauuccc caccacacug ggcaugaaua agugcuacau ccaaaucaug      180

gaccugggcc acaugugcga cgccacaaug uccuacgaau gucccaugcu cgacgaaggc      240

gucgagcccg acgacguaga cugcuggugc aacaccacua gcacgugggu cgucuacgga      300

accugccacc acaagaaagg cgaagcccgc cgaucaagaa gggcagucac ccuucccuca      360

cacagcaccc gaaaauuaca aacaagaagc caaacauggc ucgaaagccg cgaauacaca      420

aaacaccuua uccgcguaga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuacu uggcucaucc accagccaaa aagucaucua ccuagucaug      540

auccuacuca ucgcacccgc cuacuccauc cgaugcaucg gcguauccaa ccgcgacuuc      600

gucgaaggaa ugaguggcgg caccugggua gacguugucc ucgaacacgg cggaugcgua      660

accgucaugg cccaagacaa accgaccguc gacauagaac ucgucucaac caccguaagc      720

aacauggcag aaguaagauc auacugcuac gaagcaacca uaagcgacau ugcgucggac      780

ucccgcugcc caacucaggg cgaagccuac cuagacaaac aaucagacac ccaauacgug      840

ugcaaacgaa cccucgugga uagaggcugg ggcaaugggu gcgggcuuuu cgggaaaggc      900

ucccugguca caugcgccaa auucgccugu agcaagaaaa ugacaggcaa auccauccaa      960

ccggaaaacc ucgaauaccg caucaugcua ucaguacacg gcagccaaca cagcggcaug     1020

auuguaaacg acacaggaca cgaaaccgau gaaaaccgag ccaaagucga aauuaccccc     1080

aacucacccc gcgccgaggc aacccuugga ggcuucggcu cccucggacu ggacugcgaa     1140

ccaagaacag gccucgacuu uuccgaccuc uacuaccuaa ccaugaacaa caaacacugg     1200

cucguacaca aggaaugguu ccacgacauc ccccuucccu ggcacgcggg agcggacacc     1260

ggcaccccac acuggaacaa caaagaagcg cuggucgagu ucaaagacgc ccaugccaaa     1320

cgccaaacag ccgucgugcu cgggagccaa gaaggagcag uucacaccgc ccuagccggc     1380

gcccuggaag cagaaaugga cggcgccaaa gggcgacucu ccuccggcca ccucaaaugc     1440

cgacuaaaga uggacaaauu acgccuaaaa ggagucuccu auagccuaug cacagccgca     1500

uucacauuca caaagauacc cgcagaaacc cuccacggca ccguaaccgu cgaaguacaa     1560

uacgcaggaa cggacggacc augcaaagua cccgcccaaa uggcagucga caugcaaacc     1620

cuaacccccg ucggacgacu cauaaccgcc aacccaguca ucacagaaag caccgaaaac     1680

ucaaaaauga ugcucgaacu cgacccaccc uucggcgacu cauacaucgu caucggcguc     1740

ggagaaaaga aaauaaccca ccacuggcac cgcuccggca gcacgaucgg gaaagcauuc     1800

gaagccacag uccgaggagc caagcgaaug gcaguccuag gcgauacagc cugggacuuc     1860

ggcucaguag guggcgcccu aaacucacuc ggaaagggaa uccaccaaau auuuggcgcc     1920

gcauucaaau cccuauucgg cggcaugucc ugguucagcc agauauuaau aggaacacua     1980

cucauguggc uagggcucaa uaccaaaaac ggcuccaucu cgcuaaugug ccuagcccuc     2040

gguggcguac ucaucuuccu cagcaccgca gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9559
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 prME-short mRNA Design 3 opt

<400>  9559
gggagaaagc uuaccaugac aagcguugga aucguaggac uacuacugac caccgccaug       60

gcugccgaag ucacacgccg aggcucagcc uacuacaugu accucgaccg aaacgacgcc      120

ggugaagcaa uaucauuccc aaccacacuc ggcaugaaca aaugcuacau ccaaaucaug      180

gaccucggac acaugugcga cgccaccaug agcuacgaau gucccaugcu agaugagggc      240

gucgaaccag acgaugucga cugcuggugc aacaccaccu ccaccugggu cguguacggu      300

accugccacc acaagaaagg cgaagcccga cgaucacgac gcgcagucac acucccaagc      360

cacagcacca ggaaacucca aacacgcagc cagacauggc ucgaaagccg cgaauauaca      420

aaacaccuaa uaagaguaga aaacuggauc uuccgaaacc caggcuucgc ccucgcagcc      480

gccgcgaucg cauggcuacu cgggagcuca accucccaaa aagucaucua ccuaguuaug      540

auacuacuaa uagcacccgc uuacuccauc cgaugcauag gcgucucaaa ccgcgacuuc      600

gucgaaggaa ugagcggagg caccugggua gacguagucc ucgagcacgg agguugugua      660

accgucaugg cucaagacaa accaacgguc gacauagagc uaguaacaac aaccgucuca      720

aacauggcag aaguccggag cuacugcuac gaagccagca ucuccgacau ggccucugau      780

ucccgcugcc caacacaagg cgaggcuuac cuagacaaac agucagacac ccaguauguu      840

ugcaaacgga cacucgucga ccgagggugg ggcaacggau gcggccucuu cggcaaaggc      900

ucccucguaa caugcgcaaa auucgcuugu ucaaagaaaa ugacaggcaa aucaauccaa      960

ccggagaacc ucgaauaccg caucaugcuc agcguccacg ggagccaaca cuccggaaug     1020

auagucaacg acaccggaca cgaaaccgac gaaaaccgag caaaagugga aaucacgccu     1080

aacagccccc gggccgaagc aacccuagga ggcuucgggu cccuggggcu ugacugcgaa     1140

ccucguaccg gccuagacuu cagcgaccuc uacuaccuaa ccaugaacaa caaacauugg     1200

cuaguacaca aagagugguu ccacgacauc cccuuacccu ggcacgccgg agcagacacc     1260

ggcacacccc acuggaacaa caaagaagcc cucguugagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg uagugguucu aggaucccaa gaaggagccg uccacaccgc ccucgccgga     1380

gcacucgaag ccgaaaugga cggcgcaaaa ggcaggcuca gcagcggaca ccucaaaugc     1440

cgacuaaaaa uggacaaacu ccgacucaaa ggcguguccu acucccugug cacagccgcc     1500

uucaccuuca ccaaaauccc cgccgaaaca cuccacggca cgguaacagu agaacuacaa     1560

uacgccggga cagauggacc augcaaaguc cccgcacaaa uggcaguaga uaugcaaaca     1620

cuaacccccg uaggccgccu uaucaccgca aaccccguca ucaccgaauc cacagaaaac     1680

uccaaaauga ugcuugaacu cgacccacca uucggcgauu cauacaucgu cauaggcguc     1740

ggagaaaaga aaaucacgca ccacuggcac aggagcggcu ccaccauagg caaagcguuu     1800

gaagccaccg ugcgcggcgc caaacgaaug gcaguccucg gagacacagc cugggacuuc     1860

ggcuccguug gcggggcacu aaacucacuc ggcaaaggca uccaccaaau cuuuggagcc     1920

gcguucaaau cacucuucgg uggcaugucu ugguucucuc agauccuaau cggcacucuu     1980

cucauguggc uaggacucaa uaccaaaaac ggcucgauca gccucaugug ccucgcacuc     2040

ggcggcguac ucauuuuccu cagcacagcc gucucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9560
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 prME-short mRNA Design 3 opt

<400>  9560
gggagaaagc uuaccaugac auccguagga aucgucggac uccugcucac aaccgccaug       60

gccgccgaag ucacaagacg aggcuccgca uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcaa ucagcuuccc aaccacccuc ggcaugaaca aaugcuacau acaaauaaug      180

gaucuaggac acaugugcga cgccacgaug agcuacgaau gccccaugcu ggacgaagga      240

guggagccag acgacgucga cugcuggugc aacaccacaa gcacaugggu uguauacgga      300

accugccacc acaagaaagg cgaggcccgc cgcagcagac gagcaguaac ucuaccaucc      360

cacuccacac gcaaacucca aacucgaucc cagaccuggc ucgaaagccg agaauacaca      420

aaacaucuca uacgcgucga aaacuggaua uucagaaacc caggguucgc acucgccgcc      480

gccgccaucg ccuggcuccu aggcagcagc acaucccaaa aagucaucua ccuaguaaug      540

aucuuauuaa uagcccccgc guacuccaua cggugcaucg gaguaucaaa ccgagacuuc      600

guagaaggaa ugagcggggg cacguggguc gacguugucc ucgaacacgg cggcugcgua      660

accguaaugg cccaagacaa accaacagua gacauagaac ucgucaccac aaccgucagc      720

aacauggccg aagugcgcuc auacugcuac gaagccucca uuuccgacau ggccucugac      780

aguaggugcc caacgcaggg agaagcauac cuagacaaac aaagcgacac ccaauacguc      840

ugcaagcgca cucuagucga ccgaggaugg ggaaacgggu gcggucuauu cggaaaaggc      900

agucuuguaa ccugcgcaaa auucgcuugc uccaagaaaa ugacaggcaa aagcauacaa      960

cccgaaaacc ucgaauaccg caucaugcuc uccguccacg gcucacaaca cucagggaug     1020

aucgucaaug acacaggcca ugaaaccgac gaaaaccggg ccaaagucga aaucaccccc     1080

aacagcccca gagccgaagc aacccuagga ggcuucggca gccuaggccu cgacugcgaa     1140

ccucgcaccg gucuggacuu cuccgaucuc uacuaccuca ccaugaacaa caaacauugg     1200

cucguccaca aagaaugguu ccacgacauc ccccucccau ggcacgccgg agccgacaca     1260

ggaacaccac auuggaacaa caaagaagca cuggucgagu ucaaagaugc ccaugccaag     1320

agacagaccg ucgugguccu cggcucacaa gaaggcgccg uacacaccgc acucgccgga     1380

gcgcucgaag ccgaaaugga cggagccaaa gguaggcuaa gcuccggaca ccugaaaugc     1440

agacuuaaaa uggacaaguu acgccucaaa ggcgucucau acucccuuug cacagccgca     1500

uucacuuuca caaaaauccc cgccgaaacc uuacacggga caguaacugu ggaagugcaa     1560

uacgccggca ccgacggacc gugcaagguc cccgcgcaaa uggcagucga caugcaaaca     1620

cuaacacccg uaggccgccu gaucacagca aacccuguua ucacagaauc aauagaaaac     1680

uccaaaauga ugcuugaacu cgacccaccg uucggagaca gcuacaucgu aauaggcguc     1740

ggcgaaaaga aaauuaccca ccacuggcac cgcucaggca gcacaaucgg aaaagccuuc     1800

gaagccacag uccggggcgc caaacgaaug gccguccucg gcgacaccgc augggacuuc     1860

ggaucaguag gaggcgcacu caacagucuu gggaaaggaa uccaccaaau auucggagcc     1920

gccuucaaga gccucuucgg aggcaugucc ugguuuagcc aaauacuaau aggcacacuc     1980

cuaauguggc uaggccucaa caccaagaac ggcagcauca gccucaugug ccucgcccuc     2040

ggaggaguac uaauauuccu auccaccgcc gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9561
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 prME-short mRNA Design 3 opt

<400>  9561
gggagaaagc uuaccaugac aaguguagga aucguggguc uccuccugac aaccgcaaug       60

gccgcagaag uaacacggcg gggguccgca uacuacaugu accucgaccg gaacgacgca      120

ggcgaggcca ucucuuuccc caccacccua ggcaugaaca aaugcuacau ccaaauaaug      180

gaccuaggac acaugugcga cgcaaccaug uccuacgaau gccccaugcu agaugaagga      240

gucgaaccag acgacgugga cugcuggugc aacaccacca guaccugggu cgucuacgga      300

acaugccacc acaagaaagg ggaggcucgc agaagccgcc gagcugucac acucccaagc      360

cacagcacca gaaaacucca aacacgaucc caaacauggc uagaaucgcg cgaguacacc      420

aaacaccuca uccgcgugga aaacuggauc uuccgaaacc caggcuucgc ccucgcagcg      480

gcagcaauag cauggcuguu aggauccucc acaagccaaa aaguaauaua ccugguaaug      540

auccuacuca ucgcacccgc auacuccauc cgaugcauag gaguauccaa ccgggacuuc      600

gucgaaggaa ugagcggagg aaccuggguc gacguggucc uagaacacgg cggaugcguc      660

accgucaugg cacaagacaa acccacagug gacaucgagc uaguaaccac caccguguca      720

aacauggcug aaguuagauc cuacugcuac gaagcaagca ucagcgacau ggcgagugac      780

agcagaugcc ccacacaagg cgaagcauac cucgacaaac aauccgauac ccaauacgua      840

ugcaaacgca cacucguaga ccgcgguugg ggcaacggcu gcggauuauu cgggaaaggg      900

agccucguua ccugcgcaaa auucgccugc agcaaaaaga ugacaggcaa aagcauccaa      960

cccgagaauc uagaauacag aauaaugcuc uccguacacg gaucccaaca cucagggaug     1020

auaguuaaug acacaggcca cgaaacagac gaaaaccgag ccaaaguuga aauaaccccg     1080

aacucaccaa gagccgaagc cacccuagga ggcuucggau cucucggacu cgacugcgaa     1140

ccccgcacag gacuagacuu cuccgaccua uacuaucuca cuaugaacaa caagcacugg     1200

cucguacaua aagaaugguu ccacgacauc ccacuacccu ggcacgcggg cgcagacaca     1260

gggacgcccc acuggaacaa uaaagaagcg cucguagagu ucaaggaugc ucacgccaag     1320

cgccaaacag ucguaguacu aggcucccaa gaaggcgccg uccacacagc ccuagcagga     1380

gcccuggaag ccgaaaugga cggaguaaag ggcagacucu cuagcgggca ccuuaaaugc     1440

cgacugaaaa uggacaaacu cagguuaaag ggcguauccu auucacugug caccgcggca     1500

uucacauuca ccaaaauccc ggccgaaaca cuccacggca caguaacggu cgaaguccaa     1560

uacgccggca cggacggacc cugcaaagua cccgcccaaa uggcagucga caugcaaacc     1620

cucacacccg ucggaaggcu aauaacagcc aaccccguca ucaccgaaag caccgaaaac     1680

agcaaaauga ugcucgaacu agacccaccc uucggcgacu cauacauagu gaucggcguc     1740

ggcgaaaaga aaaucacgca ccacuggcac agaucaggau cgacaauagg aaaagccuuc     1800

gaagcgacag uccgcggagc aaaacgaaug gccguauuag gggacacagc augggacuuc     1860

ggcuccgucg gcggcgcacu caacucccuc gguaaaggaa uacaccaaau auucggugca     1920

gcuuucaaau cacuauucgg cggcaugucc ugguucagcc aaauucucau cggaacacuc     1980

cucauguggc ucggccuuaa cacuaaaaau ggcucaaucu cguucaugug ccucgcccua     2040

ggcggagucc ugaucuuccu cuccaccgcg guauccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9562
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 prME-short mRNA Design 3 opt

<400>  9562
gggagaaagc uuaccaugac aagcguagga auagucggac uacuacucac aacggcaaug       60

gcagccgaag ucacccgaag agggagcgcc uauuacaugu accuagacag aaacgacgca      120

ggagaagcaa ucucauuccc cacgacacuc gggaugaaca aaugcuacau acaaauaaug      180

gaccuaggac auaugugcga cgcgacaaug ucauacgaau gcccaaugcu cgacgaagga      240

guggaacccg acgacguaga cugcuggugc aacaccacau ccaccugggu gguauauggc      300

acaugccacc acaagaaagg cgaagcacgc cgaagccgcc gagcagucac acucccaagc      360

cacagcaccc gcaaacucca gacaagaagc caaacauggc uagaaucccg agaauacacu      420

aaacaccuaa uacggguaga aaacuggaua uucagaaacc caggauucgc acuugcagcc      480

gcagccaucg cuuggcuccu aggaagcucc acgucccaaa aagugaucua ucucgucaug      540

auacuacuga ucgcccccgc cuacagcaua cgcugcauag gcguaagcaa ccgagauuuc      600

guggaaggaa ugagcggcgg caccugggua gacgucguuc uagaacacgg aggcugcgua      660

accguaaugg cccaagacaa acccaccgug gacaucgaac ugguuacaac caccguaucc      720

aacauggccg agguuagaag cuacugcuac gaagcgucca ucucagacau ggccucagac      780

ucacgcugcc ccacacaagg agaagcauac cucgacaaac aaucagauac gcaguacguc      840

ugcaaaagaa cacuggugga ccgcggcugg ggaaacggau gcggccuguu cgguaaaggc      900

agccucguca caugcgcaaa auucgcgugc ucgaaaaaga ugacaggcaa aagcauacaa      960

cccgaaaacc uagaauaccg aaucaugcua uccguccacg gcucacaaca cucuggaaug     1020

aucgucaacg acacgggcca ugaaacggac gaaaacagag ccaaagucga gauaacaccc     1080

aacagcccca gggccgaagc gacacucgga ggcuucggua gccugggacu agacugcgaa     1140

ccgcgcacag gccuggacuu cagcgaccuu uacuaccuga ccaugaauaa caaacacugg     1200

cucguccaca aggaaugguu ccacgacauc ccccugccau ggcaugccgg cgcagacacc     1260

ggcaccccac acuggaacaa caaagaagcc cuaguagagu ucaaagacgc acacgcaaaa     1320

cgccagacag uaguaguccu gggcucacag gaaggcgcag ugcacaccgc acuggcaggc     1380

gcgcuggaag ccgagaugga cggcgccaaa gguaaacuau cuucgggcca ccucaaaugc     1440

cgucuaaaaa uggacaaacu aagacuaaaa ggggucuccu acagccucug caccgcagcc     1500

uucaccuuua caaaaauacc agcagaaaca cuccacggga caguaaccgu cgaagugcag     1560

uacgccggca cagacggccc cugcaagguc cccgcacaga uggcagucga caugcagaca     1620

cucaccccag uaggacgccu cauaaccgca aaccccguca ucaccgaaag caccgagaac     1680

aguaaaauga ugcucgaauu ggacccacca uucggagaca gcuacaucgu aauaggaguu     1740

ggcgaaaaga aaaucacaca ccauuggcac agaagcggga gcaccauagg caaagcauuc     1800

gaagccacgg uccgaggcgc aaaacggaug gcagugcuag gcgacaccgc augggacuuu     1860

ggaagcgugg gcggagcacu gaacagucua ggaaaaggca uacaccagau auucggagca     1920

gcguucaaaa gccuguuugg cggaauguca ugguucucgc aaauccucau aggcacccua     1980

cuuauguggc uagggcucaa caccaaaaau ggaagcauca gccuaaugug ccuagcacua     2040

gggggcgucc uaauauuccu cagcaccgcc guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9563
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY317939.1 prME-short mRNA Design 3 opt

<400>  9563
gggagaaagc uuaccaugac gagcgucgga aucgucggac uccuccuaac caccgcaaug       60

gcggccgaag ucacccgccg aggcagcgca uacuacaugu accuagaccg aaaagacgcc      120

ggagaagcga ucagcuuccc caccacacua ggaaugaaca aauguuacau acaaaucaug      180

gaucugggac acaugugcga cgccaccaug agcuacgaau gccccaugcu cgacgaaggc      240

guagaaccgg acgacguaga cugcuggugc aacaccacca gcaccugggu cguauacgga      300

accugccacc acaagaaagg agaagcacgg cgaagccgac gcgccgucac acuccccuca      360

cacagcaccc gcaaacucca aacccgaucc caaaccuggc ucgagagcag agaauacacg      420

aaacacuuaa uacgaguaga gaacuggaua uuccgcaacc ccggauucgc acuagcagca      480

gccgccaucg ccuggcuacu cggcucaucc acaucacaaa aagucaucua ccuggucaug      540

auacuacuaa uagcacccgc cuacagcaua cgcugcauag gaguaagcaa ccgcgacuuc      600

gucgaaggaa uguccggcgg caccugggua gacguagucc uggagcacgg cggcuguguc      660

accguaaugg cacaagacaa acccaccgua gacaucgagc uaguaacaac caccgucagu      720

aacauggcag aaguacgauc cuacugcuac gaagcaagca ucuccgacau ggcgucagac      780

ucccgguguc ccacgcaagg agaagcauac cucgacaagc aaagcgacac acaauacguc      840

ugcaaacgaa cccucgucga ccgaggaugg ggcaacgguu gcggccuguu cggaaaagga      900

ucccucguga ccugcgcaaa auucgcaugc agcaagaaaa ugaccggcaa gagcauccaa      960

cccgaaaacc uagaauaccg caucaugcug ucaguccacg gcagccaaca cagcgggaug     1020

auagucaacg acacuggcca cgaaaccgac gaaaacagag caaaagugga gauaacccca     1080

aacagccccc gagccgaggc aacacucgga ggauucggga gccucggccu cgacugcgaa     1140

cccaggaccg gacucgacuu cagcgaccuc uacuaccuga ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacaua ccucugccuu ggcacgcagg cgccgacacg     1260

ggaacuccac acuggaacaa caaagaagcc cucguagagu ucaaggacgc ccacgcgaaa     1320

aggcaaacgg uaguuguacu aggcagucaa gaaggcgcag uacacaccgc ccuagccggc     1380

gcccuagaag ccgaaaugga cggcgccaaa ggccgucuau ccagcggaca cuuaaagugc     1440

cgacuaaaaa uggacaaacu ucgccucaaa ggggucucau acagucucug caccgcugcg     1500

uuuaccuuca ccaaaauccc cgcagaaaca cuccacggaa cugucacagu cgaagugcaa     1560

uacgccggga cagacggccc cugcaaagua ccagcacaga uggccgucga caugcagacc     1620

uuaacaccug ucggaagacu caucacagcc aaccccguca uaaccgaauc cacagaaaac     1680

agcaaaauga uguuagaacu cgacccuccc uucggagacu cauacauagu aaucggcgug     1740

ggcgaaaaga aaaucaccca ccacuggcac agauccggau ccaccaucgg aaaagccuuc     1800

gaagccaccg uccgaggcgc caaacgcaug gccguauugg gagacacagc augggacuuc     1860

ggguccguag gaggcgccuu aaacucgcua gggaaaggca uccaccaaau cuucggagcc     1920

gccuucaaaa gccucuucgg uggcaugagc ugguucuccc aaauccucau cggcacgcuc     1980

cuaauguggc uaggucugaa caccaagaac ggcucaauau cccuaaugug ccucgcacua     2040

ggcggagucc uaauauuccu aucaaccgcc gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9564
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 prME-short mRNA Design 3 opt

<400>  9564
gggagaaagc uuaccaugac cuccguggga aucguaggac ugcuacucac aacagccaug       60

gcagcagaag ucacccgaag aggcucugcg uacuacaugu accuagaccg caacgacgcc      120

ggggaagcga uaucauuccc aacaacacuc ggcaugaaca aaugcuacau ccaaauaaug      180

gaccuaggcc acaugugcga cgccacaaug agcuacgaau gcccaaugcu cgacgaagga      240

guggagccag augaugugga cugcuggugc aacacgaccu caaccugggu aguauacggc      300

acaugccacc acaaaaaggg cgaggcccgc agaagccgca gagcagucac ccuacccuca      360

cacucuacaa gaaaacuaca aacacgcucc caaacauggc uagaaagcag agaauacaca      420

aaacaccuaa uacgaguaga gaacuggauc uuccgaaacc caggcuucgc auuggcagcc      480

gccgcaaucg cuuggcuacu aggguccagc accagccaaa aagucaucua ccuaguuaug      540

auacuccuca ucgccccagc cuacagcauc cgaugcauug guguaucaaa cagggacuuc      600

guagaaggaa ugagcggcgg aacaugggua gacguggucc ucgaacacgg cggcugcgua      660

acgguaaugg cccaagacaa acccacagua gauauagagc uagucacaac gacaguaagc      720

aacauggccg aaguaagaag cuacugcuac gaagcaagca uaagcgacau ggccucggac      780

uccaggugcc ccacgcaggg cgaagcauac cuagacaagc agagugacac ccaauacguc      840

ugcaagcgca cgcuaguaga cagaggaugg ggaaacgguu gcggccuauu cggaaaaggg      900

ucacucguga ccugcgcaaa guucgccugc aguaaaaaga ugacaggcaa aagcauacaa      960

cccgagaacc uggaauaccg uaucaugcuc agcguacacg gcucacaaca uagcgggaug     1020

auagucaacg acacaggcca cgaaacggac gagaacagag caaaagucga aauaacccca     1080

aacucacccc gagcagaagc aacccuaggc ggcuucggau cccugggacu agacugcgaa     1140

cccagaacag gccuugacuu uuccgaucug uauuaccuga caaugaacaa caagcacugg     1200

cuaguacaca aagaaugguu ccacgacaua ccccuacccu ggcacgcagg cgccgacacc     1260

ggcacacccc acuggaacaa caaagaagcc cucguagagu ucaaagaagc ccacgcaaag     1320

cgccaaacgg uagucguacu ggggagccaa gagggagccg uacacacagc gcuagcggga     1380

gcacucgaag cagaaaugga cggggccaaa gggagacuua gcagcggcca ccuaaaaugc     1440

cgacuaaaaa uggacaaacu ccgccuaaag ggaguaucau acucccugug cacagcagca     1500

uucaccuuca ccaagauccc cgcagaaaca cuccauggca cagucacagu agaaguacag     1560

uacgccggca ccgacggacc cugcaaagua ccagcacaaa uggccguaga caugcaaaca     1620

cuaaccccag uaggcagacu caucacagcu aauccaguaa uaacagaaag caccgagaac     1680

aguaaaauga ugcuggagcu cgacccgccc uucggcgacu ccuacaucgu aauaggagug     1740

ggcgagaaaa agauaacaca ccauuggcac agaagcggca gcaccauagg caaagcauuc     1800

gaagcgacgg ugcgcggggc uaaacgcaug gcagugcuag gcgacaccgc gugggacuuc     1860

ggcucaguag gaggggcacu aaacucacuc ggaaaaggca uacaccagau cuucggagca     1920

gccuuuaaga gccucuucgg aggcaugagc ugguucucgc aaauacucau cggcacacuu     1980

cucauguggc ucgggcuaaa caccaaaaac ggaucaauca gccucaugug ccuagcacua     2040

ggaggggucc ucaucuuccu aagcacggcg gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9565
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 prME-short mRNA Design 3 opt

<400>  9565
gggagaaagc uuaccaugac cagcguugga auagucggcc uacuacuaac cacagcaaug       60

gccgccgaag ucacacgcag aggguccgcu uacuacaugu accuagaccg aaacgacgcg      120

ggcgaagcaa ucagcuuccc caccacccuc ggaaugaaca aaugcuacau ccaaaucaug      180

gaccuaggac acaugugcga cgcgacuaug aguuacgaau gccccaugcu cgacgaagga      240

gucgaaccgg acgacguaga cugcuggugc aacaccacgu ccaccugggu aguauacgga      300

accugccacc acaagaaagg cgaagcacgc cgaagccgcc gcgccgucac gcugccaucu      360

cacucaacac gaaaacuaca aacacgaagu cagacauggc uagagagccg cgaguacacc      420

aaacaccuga uaagaguuga aaauuggaua uucagaaacc caggcuucgc gcuggcggca      480

gcagccaucg ccuggcuccu cggaucaucc accagccaga aagucauaua ccuaguaaug      540

auccuccuca ucgcaccagc guacagcauc cgcugcaucg gcguaagcaa ccgcgauuuc      600

guagaaggca ugagcggagg caccugggua gacguaguac ucgaacacgg aggaugcgua      660

accgucaugg cccaagacaa acccacaguc gacaucgaac ucgucaccac cacaguaagc      720

aacauggccg agguccgcag cuacugcuac gaggcaagca ucagcgacau ggcaucggac      780

ucccgaugcc caacccaagg ggaagcguac cuagauaaac aaagcgacac acaauacguc      840

ugcaaacgca cacuuguaga ccgaggaugg gguaacggau gcggacucuu uggcaaaggc      900

agccucguca caugcgccaa auucgcaugc ucaaagaaaa ugacaggaaa auccauccag      960

cccgagaacc uggaguaccg caucaugcuc aguguccacg ggagccaaca cucaggcaug     1020

aucgucaacg acacgggcca cgaaaccgac gaaaaccgag ccaaagucga aaucaccccc     1080

aacucgccaa gagcagaagc cgcccucggc ggcuucggau cacucggacu ggacugcgaa     1140

cccagaacug gacucgacuu cucagaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacaua ccccuacccu ggcacgccgg ugcugacaca     1260

ggcacuccac acuggaacaa caaagaagcc cuaguagagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg uagucguacu agggagccaa gaaggcgccg uacacaccgc acuagcaggc     1380

gcacuagaag cagaaaugga cggcgcaaaa ggcagacugu cuagcggcca cuuaaaaugc     1440

cgucucaaaa uggacaaacu gagacucaaa ggaguaagcu acucccuaug cacagcagca     1500

uucacauuca caaaaauacc cgcagaaaca cuacacggca caguaaccgu cgagguacaa     1560

uacgcaggga cagacgggcc cugcaaaguc ccagcacaaa uggccgugga uaugcaaacc     1620

cucacacccg uaggacgacu aauaaccgca aacccaguca uuacagaauc caccgaaaac     1680

agcaaaauga ugcucgaacu agacccacca uucggcgacu cauacaucgu cauaggaguc     1740

ggggaaaaga aaaucacaca ccacuggcac cgcagcgggu cgaccaucgg caaagccuuc     1800

gaagcgacag uuagaggggc aaaaaggaug gccguccuag gcgacaccgc gugggacuuc     1860

ggcuccguag gcggagcccu aaacagucuc ggcaaaggaa uacaccaaau cuucggagca     1920

gcguucaaau cacuauuugg cggcaugucu ugguucucac aaacccuaau cgggacccuc     1980

cucauguggc ucggccuaaa caccaaaaac ggcuccauca gccucaugug cuuagcccuc     2040

ggcggggucc uaauauuccu uuccacagca gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9566
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 prME-short mRNA Design 3 opt

<400>  9566
gggagaaagc uuaccaugac cagcguagga aucgucggcc uccuacuuac caccgcaaug       60

gccgcagaag ucacccgucg aggcucagcc uacuacaugu accucgacag aaacgacgcc      120

ggcgaagcca ucagcuuucc gacaacgcuu ggcaugaaca aaugcuacau acaaauaaug      180

gaccucggcc acaugugcga cgcaacgaug ucauaugaau gcccaaugcu agacgaaggc      240

guagaaccag acgacgucga cugcuggugu aacacaacau ccaccugggu ggucuacgga      300

accugccacc acaagaaagg agaagcucga cgaagccgac gcgccguaac acugcccagc      360

cauuccacgc gcaaacucca aacacgcucc caaaccuggc uugagagccg agaguacaca      420

aaacaccuaa uacgcgugga aaacuggaua uuccgaaacc ccggcuucgc acucgccgca      480

gccgcaauag cguggcuucu aggaagcucc accucacaga aagugauaua ucugguaaug      540

auacugcuca uagcacccgc cuacagcaua agaugcaucg gcgucucaaa cagggacuuc      600

gucgaaggaa ugagcggagg gacguggguc gacgucgucc uagaacacgg gggcugcgua      660

acagucaugg cgcaagacaa gcccacggua gacaucgaac uggucaccac cacagucuca      720

aacauggccg aaguacgcuc cuauugcuau gaagccucaa ucucagacau ggcguccgac      780

agccgcugcc ccacccaagg cgaagccuac cucgacaaac aaucggacac ccaauacguc      840

ugcaaaagaa cacuuguaga caggggaugg ggcaacggau gcggacucuu cggcaagggc      900

agccuaguaa caugcgccaa auucgccugc ucaaagaaaa ugaccggaaa guccauccaa      960

cccgaaaacc uagaauacag aaucaugcua uccguacacg gcagccaaca cuccggcaug     1020

aucgucaacg acaccggcca cgaaacagac gaaaaccgag ccaaggucga aauaaccccc     1080

aacucaccca gggcggaagc aacacuuggc ggauucggca gccucggccu agacugcgaa     1140

ccacgcaccg gccuagacuu cagcgaccuc uacuaccuaa caaugaacaa caaacacugg     1200

cucguacaua aggagugguu ccaugacaua ccgcuacccu ggcacgcagg cgccgacacc     1260

ggaaccccac acuggaacaa caaagaagcg cucgucgagu ucaaagacgc ccacgccaaa     1320

cgccagaccg uagucguccu aggaucacaa gaaggcgcug uacacacagc ccucgccgga     1380

gcccucgaag ccgaaaugga cggagcaaaa ggccgccuca gcagcgggca ccuaaaaugc     1440

cgacuaaaaa uggacaaacu ccgucucaaa ggaguaagcu acucccucug cacagccgcu     1500

uucaccuuca caaaaauacc agccgaaacc cuccacggca ccgucacagu agaaguccaa     1560

uacgcagggg cugacggacc cugcaaaguc cccgcccaaa uggccguaga caugcaaacg     1620

cucaccccag ucggcagacu uauaaccgcg aaccccguaa ucaccgaaag caccgaaaac     1680

ucaaaaauga ugcucgagcu agacccaccc uucggcgaca gcuacaucgu caucggcgug     1740

ggagaaaaga agaucacaca ccacuggcac agaagcggcu cgacaauagg caaagcauuc     1800

gaagccaccg uacguggcgc gaaacgcaug gccgugcucg gagacaccgc uugggacuuc     1860

gggagcguag ggggagcccu caacucccuc ggcaagggaa uacaccaaau auucggcgcu     1920

gccuucaaga gccucuucgg cggaaugagu ugguucuccc aaauacucau cggaacucuc     1980

cuaauguggc ucggacucaa caccaaaaac ggcucgauuu cccucaugug ccuugcacuu     2040

ggcggugucc uuauauuucu cuccaccgcc guaucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9567
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 prME-short mRNA Design 3 opt

<400>  9567
gggagaaagc uuaccaugac cagcgucggc auuguaggcc uauuacuuac aacagcuaug       60

gccgccgaag ucacaagacg gggauccgcc uacuacaugu accuagaccg caacgacgcc      120

ggagaagcca uaucauuccc cacaacccua ggcaugaaca agugcuacau acaaauaaug      180

gaccuugguc acaugugcga cgccacaaug agcuacgaau gucccaugcu cgacgaaggc      240

guagagcccg acgacgucga cugcuggugc aacacaacau ccacgugggu aguauacggg      300

accugccacc acaagaaagg cgaggccaga cggucacgac gagccguaac ccuacccucc      360

cacuccaccc gaaaacucca aacacgcagc caaacuuggc uagaaucacg agaauacacc      420

aaacaccuaa uccgcguaga gaacuggaua uucagaaacc caggauucgc acuagccgcc      480

gccgcaauag ccuggcuccu aggcucaucg accucacaga aaguaaucua ccucguaaug      540

auacuucuca ucgcacucgc cuacagcauc cgcugcaucg gcguaagcaa ucgcgacuuc      600

gucgagggaa ugucaggagg aacauggguc gacgucgugc uagagcacgg cgggugcgua      660

acggucaugg cacaagacaa acccaccguc gacaucgaac ucguaacgac cacagucucu      720

aacauggccg aaguacgaag uuacugcuac gaagccucca uaucggacau ggcaagcgac      780

ucccgcugcc cgacccaagg agaagccuac cuggacaaac agagcgacac acaauacgug      840

ugcaaacgaa cucuagucga ccgcggaugg ggcaacggau gcggccucuu cggaaaagga      900

agccuaguaa caugcgcgaa auucgcuugc uccaagaaaa ugaccgggaa auccauccaa      960

ccugagaacc uggaauaucg aauaaugcua uccguacacg gcucccaaca cuccggcaug     1020

aucgucaacg acacuggaca ugaaacagac gaaaaccgcg ccaaagugga aaucacaccc     1080

aacuccccac gcgcggaagc aacgcucgga ggauucggcu cacuaggccu agacugcgaa     1140

cccagaaccg gacuagacuu cagcgaccuc uacuaccuca caaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccccuaccau ggcacgcagg ggcggacacg     1260

ggaacacccc acuggaacaa caaagaagcu cucguagagu ucaaagacgc gcaugcgaaa     1320

cgacaaaccg uaguaguccu cggaucgcaa gaaggagcag uacacaccgc ccuggccggc     1380

gcccuagaag ccgaaaugga cggagcaaaa ggccgacuca gcagcgggca ccucaaaugc     1440

agacuaaaaa uggacaaacu aagacucaaa ggcguaagcu acagccuuug cacagcagcc     1500

uuuacauuca caaaaauucc cgcugaaaca cuccacggca ccguaaccgu agaaguccaa     1560

uacgcaggca ccgacggacc augcaaaguc ccagcacaga uggcugucga caugcaaaca     1620

cucacacccg uaggacgacu aauaacugcc aaccccguga ucaccgaaag cacagaaaac     1680

agcaagauga ugcucgaacu ggacccaccc uucggcgacu ccuauaucgu cauuggaguc     1740

ggagaaaaga aaaucaccca ccacuggcac cgcagcggcu ccacaaucgg caaggccuuc     1800

gaagcaaccg uaagaggcgc caaaagaaug gccguacuag gagacacggc augggacuuc     1860

ggcagcguag gcggggcacu caacagucua ggaaagggca uccaccaaau auucggcgca     1920

gccuucaaau cacuauucgg cggcaugagc ugguucaguc aaauacucau aggcacgcug     1980

cuaauguggc ucggccucaa uaccaaaaac ggaucgaucu cccucaugug ccucgcccuu     2040

ggaggcgugc uaaucuuccu auccaccgca gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9568
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 prME-short mRNA Design 3 opt

<400>  9568
gggagaaagc uuaccaugac cuccguagga auagucggcc uccuccugac cacagccaug       60

gccgccgaag ugaccagacg cggcucagca uacuacaugu accucgacag gaacgacgcc      120

ggcgaagcca ucucauuccc gaccacauua ggcaugaaca aaugcuacau ccaaaucaug      180

gaucuaggcc acaugugcga ugccaccaug ucauacgaau gccccaugcu cgaugaaggc      240

gucgaaccgg acgacguaga cugcuggugc aacaccaccu ccaccugggu cguauacgga      300

accugccacc acaagaaagg ugaagcacgc agaagccgac gcgccgugac acuucccuca      360

cacuccaccc gaaaacuaca aacacgcuca caaaccuggc uagaaucccg cgaauacacc      420

aaacaccuca uacgcgucga aaacuggauc uuucgaaacc caggauucgc ucucgccgca      480

gccgcaaucg cauggcuucu cggaucuucc acaucccaaa aagucaucua cuuaguuaug      540

auccuccuca ucgccccagc cuacuccaua cgaugcaucg gcguaagcaa ccgcgacuuc      600

gucgaaggca ugucgggagg cacgugggua gacgucguuc uagaacacgg uggcugcgua      660

acaguaaugg cccaagacaa gcccaccguc gacauugaac ucgucaccac cacuguauca      720

aacauggcug aaguccgcag cuacugcuac gaagcaagca ucuccgacau ggucuccgac      780

agccgcugcc caacccaagg cgaagcguac cuagacaaac agucggacac ccaauacguc      840

ugcaaacgca cccuagucga cagagguugg ggcaacggau gcggacucuu cggcaaggga      900

ucccuaguaa caugugccaa auucgcgugc ucgaagaaaa ugaccggaaa aagcauccag      960

cccgaaaacc ucgaauaccg cauaaugcuc uccguccacg gaagccaaca cuccggcaug     1020

aucgugaacg acaccggaca cgaaaccgac gaaaaccgcg cuaaaguaga aaucacacca     1080

aacucacccc gcgcagaagc aacccuagga ggcuucggau cccucggccu agacugugaa     1140

ccccggaccg gccuagauuu cuccgaccuc uacuaccuaa ccaugaacaa caaacacugg     1200

cucguacaua aagagugguu ucacgacauc ccccuccccu ggcacgcggg cgcagacaca     1260

gguacacccc auuggaacaa caaagaagcc cuugucgagu ucaaagacgc acacgccaaa     1320

agacaaaccg ucgucgugcu uggcucccag gaaggagccg ugcacacagc ccuggccgga     1380

gcgcuagaag cagaaaugga cggcgccaaa ggaagacuaa gcuccggaca ccuaaaaugc     1440

cgacuaaaaa uggacaaacu ucgacuuaaa ggagugucau acucacucug cacggccgca     1500

uuuaccuuca ccaaaauccc cgcagaaaca cuacacggca ccgucaccgu cgaaguacaa     1560

uacgccggaa ccgacggccc augcaaggua ccagcacaaa uggccgucga caugcagaca     1620

cuuaccccgg uaggccgacu gauaaccgcu aacccaguca ucaccgaauc aaccgaaaau     1680

ucgaaaauga ugcuggaacu cgacccaccc uucggagacu cauauaucgu cauaggcgua     1740

ggagaaaaga aaaucaccca ccacuggcac cgcucuggca gcacaauagg aaaagccuuc     1800

gaagccacag ucagaggcgc caaacgcaug gccgugcucg gcgacaccgc cugggacuuc     1860

ggaagcgucg gaggcgcccu caacagccug ggcaaaggaa uccaccaaau auucggagca     1920

gccuuuaaau cccuuuucgg aggaauguca ugguucuccc aaauucuaau uggcacacuc     1980

cuaauguggc ucggacuaaa caccaaaaau ggcucaauaa gccugaugug ucuagcacua     2040

ggaggcguac ucaucuuccu uagcaccgcc gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9569
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 prME-short mRNA Design 3 opt

<400>  9569
gggagaaagc uuaccaugac cuccguaggc auagucggcc uacuccugac aaccgcaaug       60

gcagccgaag ucacacgccg cggaagcgcc uacuacaugu accucgaccg aaaugacgcc      120

ggcgaagcca ucagcuuccc caccacgcuc ggcaugaaca aaugcuacau acaaaucaug      180

gaccucggcc acaugugcga cgccaccaug ucguacgaau gccccaugcu agacgaaggc      240

gucgaaccag acgacguaga cugcuggugc aacaccacau cgacaugggu aguguacgga      300

acaugccacc auaagaaagg agaagcaaga cgcagcaggc gagcggucac ccuacccagc      360

cacuccacga gaaaacucca gacacgcucc caaacauggc ucgaaagcag agaauacacu      420

aaacaccuca uccgaguaga aaauuggaua uuuagaaacc caggcuucgc gcuagcggca      480

gccgcaaucg ccuggcuacu cgguaguucc acaucacaaa aaguaaucua ccucguaaug      540

auccuccuaa uugcaccagc auacuccaua agaugcaucg gcaucuccaa cagagacuuc      600

guggaaggca ugucaggggg aacaugggua gacguuguac ucgagcacgg cggcugcgua      660

accguaaugg cccaagacaa accaacagug gacaucgaac ucguuaccac aaccgucagu      720

aacauggccg aaguccguuc cuacugcuac gaggccagua uauccgacau ggccagcgac      780

agccgcugcc caacccaagg ggaagccuac cucgacaaac aaucagacac ccaauauguu      840

ugcaaaagaa cccuagucga ccgaggcugg ggaaacggau gcggacuuuu cggcaaaggc      900

ucgcucguca caugcgccaa auucgcaugc uccaagaaaa ugaccggcaa aagcauacaa      960

ccagaaaacc ucgaauaccg caucaugcuc uccguacacg guagccaaca cagcgggaug     1020

aucgucaacg acacaggcca cgaaaccgac gaaaaccgcg cgaaagucga aauaaccccu     1080

aacucacccc gugccgaagc aacacuuggc ggguucggua uccugggccu cgacugcgaa     1140

cccagaaccg gccucgacuu cuccgaccuc uacuaccuua ccaugaauaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacaua ccgcuccccu ggcacgccgg agccgacaca     1260

ggcacacccc acuggaacaa caaagaagcc cugguagagu ucaaggacgc gcacgcaaaa     1320

cgacagaccg uagucgugcu cggcagucaa gaaggugcug ugcacacggc acucgccggu     1380

gcacuggaag cagaaaugga cggagcaaaa ggccgccuau ccagcggcca ucucaaaugc     1440

cgacucaaaa uggacaaacu acgucuaaaa ggagugucgu acagccuaug cacagccgcc     1500

uucacauuca cgaaaauccc agccgaaaca cuacacggaa cgguaacagu agaaguacaa     1560

uaugcaggca ccgacggacc augcaaagua cccgcccaaa uggccguuga caugcaaacu     1620

cuaacuccag uaggacgccu gauuaccgcc aacccaguca ucaccgaaag caccgaaaac     1680

ucuaaaauga ugcucgaacu ggacccacca uucggugaca gcuacaucgu cauaggcgua     1740

ggagaaaaga aaaucaccca ccacuggcac cgcagcggcu ccaccauagg caaagcauuc     1800

gaagcaaccg uccggggagc caaacgcaug gcaguccucg gcgacaccgc cugggauuuc     1860

ggguccgucg gcggggcccu caauucccug gggaaaggaa uacaccaaau cuucggggcc     1920

gccuucaaaa gccuuuucgg cggaauguca ugguucucuc aaauacucau cggaacccua     1980

cuaauguggc uuggccugaa caccaaaaac ggaagcaucu cacucaugug ccucgcacuu     2040

ggaggcgugc ucaucuuccu auccaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9570
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312313.1 prME-short mRNA Design 3 opt

<400>  9570
gggagaaagc uuaccaugac aagcguaggc aucguaggac uccuccuaac aaccgccaug       60

gcagcagaag uuacacgccg gggaucagca uacuacaugu accuagaccg caacgacgcc      120

ggugaagcca ucucguuccc cacaacacug ggcaugaaca aaugcuacau ccaaaucaug      180

gaccucggac auaccugcga cgcaacaaug ucauaugaau gccccaugcu agacgaaggc      240

gucgaacccg acgacguaga cugcuggugc aacaccacca gcacgugggu agucuauggc      300

accugucacc acaagaaagg cgaagcacgc cguucucgca gagcagucac ccuacccuca      360

cacagcaucc gcaaacuuca aacccgcucc caaacauggc ucgagucccg ggaauauacg      420

aaacaccuca uacgaguaga aaacuggauc uuccgcaacc ccggcuuugc ccuagccgca      480

gcagcaauag ccuggcuauu aggcuccagc accagccaaa aaguaaucua ccuggucaug      540

auccuccuca uugccccugc auacucaaua cgcugcaucg gagucucaaa cagggauuuc      600

gucgagggaa ugucgggcgg aacguggguc gacgucgucc ucgaacacgg uggcugcgug      660

acagucaugg cccaagacaa accgacaguu gacauagaac uugucaccac aacaguauca      720

aacauggcgg aaguccgcag cuacugcuac gaagccucaa uauccgacau ggcaagcgac      780

agccgaugcc caacccaagg cgaagcauac cucgacaaac aauccgacac gcaauaugua      840

ugcaaacgca cccuaguaga ccggggcugg ggaaacggcu gcggacucuu cggcaaaggc      900

agccuaguca caugcgcuaa auucgccugu agcaagaaaa ugacaggcaa aagcauccaa      960

cccgaaaauc ucgaguaccg aaucaugcua agcguacacg gcucacaaca cucaggaaug     1020

auagucaacg acacaggaca cgagacagac gaaaaccgcg caaaagucga gauaacgccc     1080

aacuccccac gcgcagaagc aacacucggc ggcuucggaa gucucggucu agacugcgaa     1140

ccccgaacag gacuagacuu cucagaccua uacuaccuga cuaugaacaa caagcacugg     1200

cucguccaca aagaaugguu ucacgacauc cccuuaccau ggcacgcagg cgcugacacc     1260

ggcacaccac acuggaacaa caaagaagca cucgucgagu ucaaagacgc ucacgccaaa     1320

cggcaaaccg ucguuguccu gggcucccaa gaaggcgcgg uacacaccgc ccucgccggc     1380

gcucucgagg cagaaaugga cggagcaaaa ggacgccucu ccaguggaca ccucaaaugc     1440

agacuaaaaa uggacaaacu acgccucaaa ggugucucau acagccucug uacagcagca     1500

uucaccuuca ccaaaauccc ggccgagaca cuccacggaa ccguaaccgu agaaguccaa     1560

uacgcaggga cagacggacc cugcaaaguc ccagcacaaa uggcaguaga caugcaaaca     1620

cuaacacccg ugggaagacu caucaccgca aaccccguca ucaccgaaag caccgaaaac     1680

agcaaaauga ugcuagaacu agacccaccc uucggcgacu cauacauagu aauaggcgug     1740

ggagaaaaga aaaucaccca ccacuggcac cgcagcggau caaccauagg caaagcauuc     1800

gaagccacag uccgcggugc aaaacgcaug gccguccucg gcgacacagc cugggacuuc     1860

ggcagcguag gcggcgcccu aaacucgcua ggaaaaggca uacaccagau cuucggggca     1920

gccuucaaga gucucuucgg cgguaugucc ugguucagcc aaauccucau cgggacccua     1980

cucauguggc ucggccucaa cgccaaaaac ggcucgaucu cacucaugug ccuagcacuc     2040

ggcggcgucc ucauauuccu gucaacagcc guaagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9571
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075935.1 prME-short mRNA Design 3 opt

<400>  9571
gggagaaagc uuaccaugac aucagucggg auagucggcc uacuacuaac cacggccaug       60

gcagccgaag uaacccgacg cggaucggca uacuacaugu accuagacag aaacgacgca      120

ggagaagcca uaucuuuccc caccacauua ggcaugaaca aaugcuacau ccaaaucaug      180

gaccuaggac acaugugcga cgcaacgaug ucguaugagu gccccaugcu cgacgaaggg      240

guggaacccg acgacguaga cugcuggugc aacacaaccu caaccugggu cguauacgga      300

accugccauc auaagaaagg ggaagcccaa cgcucccgca gagccguaac acuacccagc      360

cacagcacca gaaaacuaca aacccgcuca caaaccuggc uggaaucacg cgaguacacc      420

aaacaccuaa uaaggguaga aaacuggaua uuccggaacc caggcuucgc ccucgccgca      480

gcagcaauag cauggcuacu cggauccuca acaucccaga aagugaucua ccucguuaug      540

auacuccuua ucgcccccgc cuacagcauc cgaugcauag gcgucagcaa ccgagacuuc      600

guagaaggaa ugagcggcgg caccugggua gacgucguac ucgagcacgg cgguuguguc      660

accgucaugg cccaggacaa accaacagua gacauugaac ucgugacaac aacggucucc      720

aacauggcag aaguacgcag cuacugcuac gaagccagca ucagcgacau ggccuccgac      780

agccgcugcc ccacacaagg agaagcguac cuggacaaac aaucagacac ccaauacguc      840

ugcaaacgca cccuagucga ccgaggaugg ggcaacggau gcggccucuu cggcaaagga      900

ucgcucguaa caugcgccaa auucgccugc agcaagaaaa ugaccggaaa aucaauccaa      960

cccgaaaacu uagaauaccg cauaaugcua ucaguccacg gcucacaaca cagcggcaug     1020

aucguaaacg acaccggcca cgaaaccgau gagaaccgcg caaaggucga aauaaccccc     1080

aacagccccc gcgccgaagc aacccuagga ggauuuggaa gccucggccu agacugcgaa     1140

ccacgcaccg gccucgacuu cuccgaccua uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aagaaugguu ccacgacauc ccccuccccu ggcacgccgg agccgacacg     1260

ggaacacccc acuggaauaa caaagaggcc cuaguagagu ucaaagacgc acacgcgaaa     1320

cggcaaacag uaguuguacu cggaucacaa gaaggggccg uacacacagc ccuagcagga     1380

gcacucgaag cggaaaugga cggagccaaa ggcagacuca gcagcgggca ucuaaaaugc     1440

cgacucaaaa uggacaaacu ccgauugaaa ggcgucagcu acagccucug uaccgcagca     1500

uuuacauuca ccaagauacc cgccgaaaca cuacacggaa caguaaccgu agaaguccaa     1560

uacgccggga cagacgggcc cugcaaggua cccgcacaga uggcagugga caugcaaacc     1620

uuaacaccgg uaggacgacu gaucacagcg aacccaguca ucacggaauc aaccgaaaac     1680

uccaaaauga ugcuggaacu agacccgccu uucggcgaua gcuacaucgu aauuggagug     1740

ggcgaaaaga aaaucacaca ccacuggcac cgcuccggcu caacaaucgg aaaagccuuc     1800

gaagccaccg uccgcggcgc caaaagaaug gcaguccuag gagacacagc augggacuuc     1860

gguagcguag gcggagccuu aaacucacug ggaaaaggca uccaccaaau cuucggagcc     1920

gcauucaaau cacuauucgg aggaaugucc ugguucaguc aaauacucau cggcacgcua     1980

cucauguggc uaggacucaa caccaaaaac ggaagcaucu cgcugaugug ccuagcccua     2040

ggaggcguac ucauauuccu auccacagcg gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9572
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014320.1 prME-short mRNA Design 3 opt

<400>  9572
gggagaaagc uuaccaugac aagcgucgga auagugggac uccuacucac aacagcaaug       60

gcggccgagg ucacacgacg aggaagcgcc uacuacaugu accuagaccg caacgacgca      120

ggagaggcca ucucguuccc cacgacccua ggcaugaaca aaugcuacau ccagaucaug      180

gaccucgguc acaugugcga cgcaacaaug ucguacgaau gccccaugcu cgacgaaggg      240

gucgaaccag acgacguaga cugcuggugc aacacaacaa gcaccugggu gguauacggc      300

accugucacc acaagaaagg agaagcccga agaagccgca gagccguaac acucccaagc      360

cacuccaccc ggaagcuaca ggcacgcagc caaaccuggc uagaaagcag agaauacacc      420

aaacaccuca uacgcgucga aaacuggaua uucagaaacc ccggauucgc ccuagccgcc      480

gccgccaucg cguggcuuuu agguuccucc acuagccaaa aaguaauaua ccuaguaaug      540

auacuccuaa uagcgccagc cuacagcauc agaugcauag gcguauccaa cagagacuuc      600

gucgaaggca uguccggagg aacauggguc gaugucguac uagaacacgg cggcugcgua      660

accgucaugg cccaagacaa accaacagua gauauagaac ucgucacuac cacgguaagc      720

aacauggccg aggugcgcuc auacugcuac gaggccagca ucucagacau ggcaucagac      780

ucacgcugcc caacacaagg agaagcauac uuagacaaac aaagcgacac acaauacgua      840

ugcaaacgca cccuaguaga uagaggcugg ggcaacgggu gcggacucuu cggcaaagga      900

ucccuaguca caugcgcgaa auucgccugc ucaaagaaaa ugacaggaaa aucaauacaa      960

ccagaaaacc ucgaauaccg caucaugcua agcgugcacg gaucacaaca cuccggcaug     1020

aucguaaacg acacaggcca cgaaacagac gaaaaccgag caaaagugga aauaacaccu     1080

aacucaccac gcgccgaagc cacccuaggg ggcuucggcu cacucggccu cgacugcgaa     1140

cccaggacag gccuggacuu uucggaccug uacuaccuua caaugaacaa caaacacugg     1200

cuaguacaua aagaaugguu ccacgacauu ccacuucccu ggcacgcagg agccgacacc     1260

ggcaccccac acuggaacaa uaaggaagca cucguagagu ucaaagacgc ccacgccaag     1320

agacagacag uagucguacu cggaucccag gagggcgcag uacacacggc ccuagcgggc     1380

gcacuagaag ccgagaugga cggcgccaaa ggccggcuca gcagcggcca ccucaaaugc     1440

cggcuuaaaa uggacaagcu cagacugaaa ggcguaucau acagccuaug caccgccgcc     1500

uucacguuca caaaaauccc agcggaaaca cuacacggaa ccgucacagu cgaagugcaa     1560

uacgcgggca cagacggccc cugcaaagug cccgcccaaa uggcggucga caugcaaaca     1620

cuaaccccag uaggaagacu caucaccgca aacccgguca uaacagaaag cacagaaaac     1680

agcaagauga ugcuagaacu agacccacca uucggcgacu cauacauagu caucggcgua     1740

ggagaaaaga aaaucacaca ccacuggcac cgaagcggcu cuacaaucgg aaaagccuuc     1800

gaagccaccg uacgcggcgc gaaacgcaug gccguccucg gagacacagc augggacuuu     1860

ggcagcguag gaggcgcccu caacucccua ggcaaaggca uccaccaaau auucggggcc     1920

gccuucaaau cccucuucgg aggaauguca ugguucagcc agaucuuaau aggaacccug     1980

cuaauguggc uaggccucaa cacgaaaaac ggguccauau cgcuaaugug ccuagcacug     2040

ggcggagucc ucauauuccu aucaacagcc guauccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9573
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 prME-short mRNA Design 3 opt

<400>  9573
gggagaaagc uuaccaugac aagcgucggc aucguaggcc uccuccugac cacagccaug       60

gcagccgaag uuacacgccg aggaucagca uacuacaugu accucgaccg cuccgacgcg      120

ggagaagcca ucagcuuccc uaccacccuc ggcaugaaca aaugcuacau acaaauaaug      180

gaccugggac acaugugcga cgcaacaaug ucguacgaau gcccaaugcu agacgaaggc      240

gucgaacccg augacguaga cugcuggugc aacaccacau ccaccugggu ggucuacgga      300

accugccacc acaaaaaggg cgaggccaga cggucgagac gcgcggucac ccuuccuagc      360

cacagcacca gaaaacuaca aacacggucg cagacuuggc ucgaaaguag agaguauacc      420

aaacaccuca uccgggucga aaauuggauc uuccgcaacc ccggauucgc gcucgccgcc      480

gccgcaauag cguggcuacu cggcagcucc acaagccaaa aagucauaua ccucguuaug      540

auacuccuca ucgcccccgc cuacuccauu cgaugcauag gcguaagcaa cagagacuuc      600

guagaaggca ugaguggagg gaccuggguc gacguagucc ucgaacacgg cggcugcguc      660

accguaaugg cgcaagacaa acccacagua gacaucgagc uagucaccac caccguaagu      720

aacauggcag aaguccgauc cuacugcuac gaagcaucaa ucuccgacau ggccuccgau      780

agccggugcc ccacacaagg cgaagcauac cucgacaagc aaagcgacac ucaguacguc      840

ugcaaaagaa cacucgucga ccgaggcugg ggaaauggau gcggccuauu cgguaaaggc      900

ucccucguaa ccugcgccaa auucgcaugu ucgaagaaaa ugaccggaaa aagcauacaa      960

cccgagaacc uagaauaccg uauaaugcua agcguccacg gaagccaaca cagcggaaug     1020

aucgucaacg acacaggcca ugaaacagac gaaaaccgcg ccaaaguaga aauaaccccu     1080

aacucaccaa gggcagaagc aacccuaggc ggauucggaa gccuaggacu cgacugugaa     1140

ccaaggacag gacucgacuu cucagaccua uacuaccuca ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacauu ccgcuaccgu ggcaugcugg cgccgauaca     1260

ggcacgcccc auuggaacaa caaagaagca cucguagagu ucaaagacgc ccacgcaaaa     1320

cgccaaaccg ucgugguccu agggagccag gaaggagccg uccacaccgc ccucgccgga     1380

gcccucgaag ccgaaaugga cggcgcaaaa ggccgcuuaa gcuccggcca ccuaaaaugc     1440

cgccugaaaa uggacaaacu caggcucaaa ggcguaagcu acucgcuaug uaccgcagca     1500

uucacauuca gcaaaauccc agcagaaaca cuacacggca cagucacaga agaaguacag     1560

uacgccggca ccgacggccc cugcaaaguc cccgcgcaaa uggccgucga uaugcaaaca     1620

cuaacacccg uaggccgacu aauaacagca aaccccguaa ucaccgaaag caccgaaaac     1680

agcaaaauga ugcuggaacu cgacccgccc uucggagacu cauacaucgu aauaggcguc     1740

ggagaaaaga aaaucacuca ccacuggcac cgcuccggca gcaccaucgg caaagccuuc     1800

gaagccaccg uccgcggcgc caagcgaaug gcgguacuag gcgacacagc cugggacuuc     1860

ggaucaguug gaggagcccu aaacucacua ggcaagggca uacaccaaau cuucggagca     1920

gccuucaaau cccuauucgg gggcaugagc ugguucagcc aaauccuaau cggaacccuc     1980

cucauguggc ucggccuaaa caccaaaaac ggcuccauua gucuaaugug ccuagcccuc     2040

ggcggcgugc ucaucuuccu auccacagcg guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9574
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 prME-short mRNA Design 3 opt

<400>  9574
gggagaaagc uuaccaugac cucagucgga auaguaggac uacuccucac gacagcaaug       60

gccgcagaag uaaccagacg cgguuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggagaagcca ucagcuuccc cacaacccua ggcaugaaca aaugcuacau acaaaucaug      180

gaucucggac acaugugcga cgccaccaug uccuacgaau gccccaugcu cgacgaagga      240

guagagccgg acgacguuga cugcuggugc aacacaacau ccacgugggu agucuacgga      300

accugccauc acaagaaagg cgaagcccga agaucccgcc gcgccgucac acuacccagc      360

cacucaacgc ggaaacucca aacaagaucg caaaccuggc uagaaagcag agaauauacc      420

aaacaccuaa ucagaguaga gaacuggaua uucaggaacc caggcuucgc acuagccgcc      480

gcagccauag ccuggcuccu cgguuccucc acaagccaaa aaguaauaua ccucgucaug      540

auacuccuca ucgcaccagc auauucgaua cguugcauag gagugagcaa uagagacuuc      600

guagagggaa ugucaggagg caccugggua gacgucguac ucgagcacgg cggaugugua      660

acaguuaugg cccaagacaa acccaccguc gacauagaac ucgucacaac cacagucuca      720

aacauggccg aaguaagauc cuacugcuac gaggcuucca ucucagacau ggcauccgac      780

ucacgcugcc caacccaagg cgaagcguau cucgacaaac agucugacac ccaguacguc      840

ugcaaacgca cacucgugga cagaggaugg ggcaacggau gcggccucuu cggcaaaggc      900

ucccuaguaa caugcgccaa auucgcaugc uccaagaaaa ugaccggaaa aagcauacag      960

cccgaaaacc ucgaauaccg cauaaugcua ucaguccacg gaucccaaca cuccggcaug     1020

aucguaaacg acaccggcca cgaaaccgac gaaaaccgcg cgaaagucga aaucaccccc     1080

aacagcccac gagccgaagc cacacuaggu ggcuucggaa gccucggacu ggacugugaa     1140

ccccgcacag gccuagacuu cagcgaccuu uacuaccuca ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccacuaccgu ggcacacagg agcagacacc     1260

ggaacaccac acuggaacaa caaagaagcc uuaguagagu ucaaagacgc ccacgcaaaa     1320

cgacaaacag ucguaguacu cggcucucaa gaaggagccg uccacaccgc acuagcaggc     1380

gcccucgaag ccgaaaugga cggagcuaaa ggcaggcuga gcucgggcca ccuuaaaugc     1440

agacucaaaa uggacaaacu uagacucaaa ggaguaucau auucccucug cacagcagca     1500

uucaccuuua ccaaaauacc agccgaaacc cuacacggaa ccgugaccgu agaaguucaa     1560

uacgcaggaa ccgacggccc augcaaagua cccgcacaaa uggcagugga caugcagacc     1620

cugacaccgg ucggacgccu cauaacagcc aaccccguca ucacugaagg cacagaaaac     1680

ucgaaaauga ugcucgaacu agacccaccc uucggcgacu ccuacauagu caucggcguc     1740

ggugaaaaga aaaucacaca ccacuggcac agaagcggcu caacaaucgg aaaagccuuc     1800

gaagccaccg uccgcggugc gaaaagaaug gcaguccucg gcgacacagc augggauuuc     1860

ggaagcguag gcggaguacu aaacucgcuc ggcaaaggca uacaccaaau cuuuggcgca     1920

gccuucaaaa gccuauucgg aggcaugucc ugguucagcc agauccuaau cggcacacuc     1980

cuaauguggc ugggacucaa uaccaaaaac gggagcauau cccucaugug ccuugcacua     2040

ggcggcguuc ucaucuuccu cuccacagcc gucagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9575
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX827309.1 prME-short mRNA Design 3 opt

<400>  9575
gggagaaagc uuaccaugac cagcgucggu auagucggcu uacuccucac aacagcaaug       60

gcagccgagg ucacccgaag aggaucagcc uacuacaugu aucuggaccg aucagacgca      120

ggggaagcaa ucuccuuccc aacaacccua ggcaugaaua agugcuacau acaaauaaug      180

gaccucggac acaugugcga cgccacgaug uccuacgaau gcccaaugcu ugacgagggc      240

guagaaccag acgacgucga cuguuggugc aacaccacgu ccacgugggu cgucuacgga      300

accugccacc auaagaaagg cgaagcgcga cguagcagac gagcagucac ucuaccaagc      360

cacagcacca gaaaacuaca aacaagaagc caaaccuggc uagaaagccg agaguacacc      420

aaacaccuaa uccgcguaga aaacuggauc uucaggaacc cuggcuucgc acucgccgca      480

gcagcaaucg ccuggcugcu cggaucaagc acaagccaaa aaguaauaua ccuugugaug      540

auccugcuaa uagcccccgc cuacuccaua cgcugcauag gagucucaaa ucgcgacuuc      600

guugaaggca uguccggcgg cacauggguc gacguagugc ucgaacacgg uggcugcguc      660

acagucaugg cccaggacaa accaacagua gacauagaac uuguaaccac uaccgucagu      720

aacauggccg agguacguuc auacuguuau gaagcaucca uaucggacau ggccucagac      780

ucccgaugcc caacccaagg cgaagcguac cucgacaaac aaucagacac acaauauguc      840

ugcaaacgca cccuagucga cagaggcugg ggaaacggau gcggacucuu cggaaaaggc      900

ucccucguaa ccugcgcaaa auucgcgugc ucaaagaaaa ugacgggcaa aagcauacaa      960

cccgaaaacc ucgaguaccg caucaugcua ucaguccacg guucacagca uagcggcaug     1020

auaguaaacg acacaggaca cgagacagac gagaaccgcg caaaagucga gauuacaccu     1080

aauagucccc gcgcagaagc cacauuaggc ggauucggaa gccugggacu agacugcgaa     1140

ccucgaaccg gacucgacuu cuccgaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacauc cccuuaccau ggcacgccgg cgcggacaca     1260

ggcaccccac acuggaacaa caaagaagcc cuagucgagu ucaaagacgc ccacgccaaa     1320

agacaaacug uagucguccu aggaagccaa gaaggagcag ugcacaccgc acuggcaggc     1380

gcccuagaag cagaaaugga cggcgccaaa ggcagacuga gcucaggcca ccuaaaaugc     1440

cgacucaaaa uggauaaacu ccgauuaaaa ggggucucau acucccucug caccgccgcc     1500

uucacuuuca caaaaauccc cgccgaaacc cuccacggua ccgucaccgu cgaaguacaa     1560

uacgccggca ccgacggccc cugcaaaguc cccgcccaaa uggccguaga caugcaaacc     1620

uuaaccccag ucggacgccu caucacagca aaucccguca ucaccgaauc caccgaaaac     1680

ucgaaaauga ugcucgaacu agacccacca uucggagaca gcuacaucgu caucggagua     1740

ggugaaaaga aaauaaccca ccacuggcac cgcagcggaa gcaccauagg aaaagccuuc     1800

gaggccaccg uccgaggagc aaaaagaaug gcgguucucg gugacaccgc augggacuuc     1860

ggcagcguag gcggagcucu gaacagccua ggaaaaggaa uccaccaaau uuucggggcc     1920

gcauucaaaa gccucuucgg aggaaugucc ugguucucgc aaauacucau uggcacacuc     1980

cucauguggc ucggacucaa cacaaagaac ggcagcauca gccucaugug ccucgcccuu     2040

gguggcgucc ucauauuccu cagcacagcc gucucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9576
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955593.1 prME-short mRNA Design 3 opt

<400>  9576
gggagaaagc uuaccaugac cagcgugggc auagucggac uccuacucac caccgccaug       60

gccgucgaag ucacgcgccg gggcaacgcc uacuacaugu accuagaccg uagcgacgca      120

ggagaggcaa uaucauuccc cacaacaaug ggaaugaaca aaugcuacau ccagaucaug      180

gaccuaggcc acaugugcga cgcaacaaug ucauacgaau gcccaaugcu cgacgaagga      240

guggaacccg acgacguaga cugcuggugc aacacaacaa gcaccugggu aguauacgga      300

acgugccacc acaagaaagg cgaagcccga cggucccggc gagccgucac ccucccaagc      360

cacuccacac gaaaauuaca aacaaggagc caaaccuggc ucgaaagccg agaguacacc      420

aaacaccuca ucagagugga aaacuggauc uuccgaaacc cuggauucgc gcucgccgca      480

gcagccauag cauggcuucu cggcuccagu accagccaga aaguuaucua ccucguaaug      540

auccuacuca ucgcuccagc auacuccauc cgaugcaucg gcgucagcaa ccgcgauuuc      600

gucgaaggua ugagcggcgg cacaugggua gacgucguac uagaacacgg cggcugugug      660

acagucaugg cucaggacaa accaacaguc gauaucgaac ucguaaccac aaccgucucc      720

aauauggcag aagucagauc auauugcuac gaagcaagca ucuccgacau ggccagcgac      780

ucacguugcc caacccaagg cgaagccuac cuggacaaac aguccgacac ccaauacgua      840

ugcaaacgaa cccucguuga ccgaggcugg ggcaacggcu gcggccucuu cggcaaagga      900

ucacucguaa caugcgccaa auucgcuugc ucaaagaaaa ugacgggaaa aagcauacaa      960

cccgaaaacc uagaguaccg uauaaugcuc uccguccaug gaucccaaca cucaggcaug     1020

aucgucaacg acacgggcca cgaaacagac gaaaaccgcg caaaagucga aauaacaccc     1080

aacagccccc gagccgaagc aacacucgga ggcuucggua gccucggccu agacugugaa     1140

ccgcgcacag gacuagacuu uagcgaccuc uacuacuuaa ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ccacgacaua ccauugcccu ggcacgcagg ggccgacaca     1260

ggcaccccac acuggaacaa caaagaagcc cucguagagu ucaaagaugc acacgcuaaa     1320

agacaaaccg ucgucguacu cggcagccaa gaaggcgccg uacacacagc ccuugcgggc     1380

gcacuagaag cagaaaugga cggagccaaa ggccgacugu cauccggcca ccucaagugc     1440

agacucaaaa uggacaaacu ccgccuaaaa ggcguuuccu acagccucug caccgccgcc     1500

uucacauuca ccaagauucc cgcggaaacu cuccauggga ccgucacagu agaaguacaa     1560

uacgcaggaa cagacggccc cugcaaaguc cccgcccaga uggccgucga caugcaaacc     1620

cugacccccg ucgggcgucu aaucacagcc aacccgguaa ucaccgaauc gaccgaaaac     1680

ucaaaaauga ugcuagaacu ugacccgccc uucggcgacu ccuacaucgu cauaggcgua     1740

ggcgaaaaga aaauaaccca ucacuggcac cgauccggua gcaccauagg gaaagccuuc     1800

gaagccaccg uacgcggagc aaaaagaaug gccguccucg gagacacagc augggacuuc     1860

ggaucgguag gcggagcacu caauucacua ggcaaaggca uacaccagau cuucggcgca     1920

gcauucaaaa gccucuucgg cggaaugagc ugguucagcc aaauccuaau aggaacccua     1980

cugguuuggc uaggccucaa caccaaaaac ggcucaauca gccucaugug ccuugcccua     2040

ggaggcgucc ucauauuucu aucaacugca gucucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9577
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY272987.1 prME-short mRNA Design 3 opt

<400>  9577
gggagaaagc uuaccaugac cuccguaggc auagucggac uccucuugac cacagccaug       60

gcagccgaag ucacccgacg cggcagcgca uacuacaugu accuagaccg uuccgacgca      120

ggcgaagcaa uuagcuuccc caccacccua ggcaugaaca aaugcuacau acaaauaaug      180

gaucucgggc acaugugcga ugccacaaug ucguacgaau gccccaugcu cgacgaagga      240

guagaacccg acgacgucga cugcuggugc aacacaacca guacaugggu agucuacgga      300

accugccacc acaagaaagg ugaagcucgg agaucccgac gcgcggucac ccuccccucc      360

cacuccacaa gaaaacugca aacaagguca caaacauggc ucgaaagcag agaauacacc      420

aagcaccuaa uaagagugga aaacuggaua uuucggaacc ccggcuucgc guuagccgca      480

gccgccaucg cguggcuccu agggagcuca acuucacaaa aaguaauaua ccucgucaug      540

auccuccuga uagccccagc cuacuccaua cguugcauag gcguaucuaa ccgcgacuuc      600

gucgaaggaa uguccggagg cacauggguc gacgugguac ucgaacaugg cggcugcgua      660

accguaaugg cacaagacaa accgacggua gacauagaac ugguuacaac caccgucagc      720

aacauggccg aaguaagauc auacuguuac gaagcaucaa ucagcgacau ggccagcgac      780

ucccgcugcc ccacccaagg agaagcguau cucgacaagc aauccgacac ccaauaugua      840

ugcaaacgca cccucgugga ccgaggaugg ggcaacggau gcggccuauu cggcaaagga      900

ucccuaguca caugcgcgaa guucgcaugc ucaaagaaaa ugaccggaaa auccauacaa      960

cccgagaauc ucgaauacag aaucaugcuc uccguacacg ggucucaaca uuccggcaug     1020

aucgugaacg acacuggcca cgaaaccgau gaaaaccgag caaaagucga gauaacacca     1080

aauucacccc gcgccgaagc cacccuagga ggcuucggaa gccuaggccu cgacugcgaa     1140

ccccgaaccg gacuggacuu cuccgaccug uacuaccuca caaugaacaa caaacacugg     1200

cuaguccaca aagagugguu ccacgacaua ccacuucccu ggcacgccgg cgccgauaca     1260

gggacacccc acuggaacaa caaagaagcc cuuguagagu ucaaagacgc acacgcaaaa     1320

cgccaaacag ucgugguccu aggcagccaa gagggagccg uacacaccgc ccuagccgga     1380

gcccuagaag ccgaaaugga cggcgccaaa ggcagauuaa gcuccggcca ccucaagugc     1440

cgacuaaaaa uggauaaacu gcgccuuaaa ggcguaagcu acucccucug uacagcagca     1500

uucacauuca caaaaauacc cgccgagaca cuccacggaa caguaacagu cgaaguccaa     1560

uacgcaggaa cagauggacc cugcaaaguc ccggcacaaa uggccguaga caugcaaacc     1620

cuaacccccg ucggcagacu cauaaccgca aaccccguca uaacagaguc aaccgaaaac     1680

ucaaaaauga ugcuggaacu cgacccaccc uucggcgaca gcuacauagu aauaggcgua     1740

ggcgaaaaga aaauaacaca ccacuggcac cgcagcggau caaccaucgg gaaagccuuc     1800

gaagccacgg uccgaggcgc aaaaagaaug gccgugcucg gcgacacagc cugggacuuc     1860

ggcagcgucg gcggagcccu caacucccua ggcaagggca uucaccaaau cuucggagcc     1920

gccuucaaaa gccucuucgg aggcaugucg ugguucuccc aaauacuaau cggcacccuc     1980

cuuauguggc ugggccucaa caccaaaaac ggcuccaucu cgcuagucug ccuagcccuc     2040

gggggagucc ucauauuccu uucgacagca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9578
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 prME-short mRNA Design 3 opt

<400>  9578
gggagaaagc uuaccaugac cucaguugga auaguggggc uacuacuaac cacagccaug       60

gcggcagaag uaacccgaag aggcagcgcc uacuacaugu accucgaccg cagcgacgcc      120

ggcgaagcaa ucagcuuccc aaccacccua ggaaugaaca aaugcuacau ccaaaucaug      180

gaccuagguc acaugugcga cgccaccaug agcuacgaau gccccaugcu ugacgaagga      240

guugaaccag acgacgucga cugcuggugc aacaccacaa gcaccugggu cgucuacggg      300

accugccacc acaagaaagg cgaagcgcgc cgaagccgca gagcugucac ccuccccagc      360

cacuccaccc gaaaacuaca aaccaggagc caaacauggc ucgaaagccg cgaauauacc      420

aaacacuuaa ucagagugga gaacuggaua uuccgcaacc ccggauucgc acucgcagcg      480

gcagcgaucg cauggcuccu aggcagcucc accagccaaa aagucaucua cuuagucaug      540

auccuccuca uagcccccgc cuacucgauc agaugcaucg gcguuaacaa ccgagacuuc      600

gucgaaggca ugucuggcgg caccuggguu gacgucgucc uagagcacgg cggcugcgua      660

accguaaugg cucaagacaa acccaccguc gacaucgagc uagucacaac caccguaagc      720

aacauggccg aaguucgaag cuacugcuac gaagcaucca uuagcgacau ggcaucagac      780

ucaaggugcc caacacaagg agaggcuuac uuagacaaac aaagcgacac gcaauacgug      840

ugcaaacgca cccuagucga ccgcggaugg ggcaacggcu gcggccucuu cgguaagggc      900

agccugguaa ccugcgcaaa auucgccugc ucaaagaaaa ugacaggaaa aagcauacag      960

cccgaaaauc ucgaauaccg uauaaugcua agcguccacg gaucacaaca cagcggaaug     1020

aucgucaacg acaccggaca cgaaacagau gaaaaccgag ccaaagucga aaucacccca     1080

aacagccccc gcgcugaagc cacccugggc ggguucggau cacuaggccu agacugcgag     1140

ccgcgcacag gccuggacuu cagcgaccuc uacuaccuaa ccaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacauc ccacucccau ggcacgccgg cgcagacacc     1260

ggaaccccgc acuggaacaa caaagaagca cucgucgagu ucaaagacgc ccacgccaaa     1320

cgccagacag ucgucguccu gggaucccaa gaaggcgccg uccacacagc acucgccggc     1380

gcccuagagg ccgaaaugga cggagcaaaa ggacgccuau caaguggcca ucucaaaugc     1440

cggcuuaaaa uggacaaacu uagauugaaa ggcgucuccu acucucucug uaccgcggcc     1500

uucacauuca caaaaauacc cgcggaaaca cuccacggca cagucaccgu cgagguccag     1560

uacgcaggga ccgaugggcc gugcaaagua ccagcccaaa uggccguaga caugcaaaca     1620

cuaacccccg ucggacgucu aauaacagcc aaccccguca uaacggaauc aacagagaac     1680

uccaaaauga uguuagaauu agacccucca uucggcgacu cguacaucgu cauaggggua     1740

ggagaaaaga aaaucacaca ccacuggcac cgcagcggaa gcaccauagg caaagcguuc     1800

gaagccaccg uacgaggcgc caaacgcaug gccguccucg gagauaccgc cugggacuuc     1860

ggaagcgucg gcggagcccu caacucucua ggaaaaggca uccaccaaau auucggagcc     1920

gcauucaaaa gccuauucgg aggcauguca ugguucuccc aaauacucau cggaacgcuc     1980

uuaauguggc uaggccucaa uaccaaaaac ggcuccauau cccuagucug cuuagcccuc     2040

ggcggcgucc uaauauuccu aagcaccgca guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9579
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 prME-short mRNA Design 3 opt

<400>  9579
gggagaaagc uuaccaugac aucaguaggg aucgucgguc ugcuccugac cacagcaaug       60

gcaguggaag ucacacgacg cggcagcgcg uacuacaugu accucgaccg aucagacgca      120

ggcgaagcaa ucuccuuccc caccacacuc ggcaugaaca aaugcuacau ccaaaucaug      180

gaccuaggac acaugugcga cgcaacaaug ucauacgaau gcccaaugcu ggacgaagga      240

guagaacccg acgacgucga cugcuggugc aacacuacca gcacaugggu cguauacggg      300

acuugccauc acaagaaagg ggaagcaaga cgcucacgac gagcggucac gcucccgagc      360

cacucaacac ggaaacucca aacucggucg caaaccuggc uagaaucaag agaguacaca      420

aaacaccuaa uacgaguaga aaacuggauc uucagaaacc caggcuucgc ccucgccgcg      480

gccgccaucg ccuggcuccu aggcagcagc accucccaaa aagucauaua ccugguaaug      540

auccuacuga uagcaccagc cuacucaauc aggugcauag gaguaagcaa ccgagauuuc      600

guagaaggaa uguccggagg cacaugggua gacguggucc uggaacacgg cggaugcgug      660

acagucaugg cccaagacaa accagccguc gauauagaac ucgucaccac aaccguaagc      720

aacauggcag aaguaagauc cuacugcuac gaagccagca uaagugacau ggcaagcgac      780

agccgcugcc ccacccaagg cgaagccuac cucgacaaac aaucagacac ccaguacgua      840

ugcaaacgca cauuaguaga cagaggcugg ggcaacggcu gcggacuauu cgggaaaggc      900

ucgcugguaa ccugcgccaa auucgcgugc uccaagaaaa ugaccggcaa aagcauacag      960

cccgaaaacc ucgaauaccg gauaauguug uccgugcacg gcagccaaca cuccggcaug     1020

aucgucaacg acaccggaca ugagacagac gaaaaccgcg ccaaaguaga aauaaccccu     1080

aacagcccgc gagccgaggc gacccucgga gguuucggcu cguuaggccu cgauugcgaa     1140

ccccguaccg gacuagacuu cuccgacuua uacuaccuca cgaugaacaa caaacacugg     1200

cuuguacaca aagaaugguu ccacgacaua ccacugcccu ggcacgccgg cgcagacaca     1260

ggcaccccac acuggaacaa caaagaggcc cuaguagaau uuaaagacgc ccaugcaaaa     1320

cgccaaacag uaguaguccu cggcagccaa gaaggcgcag ugcacacagc acuagccgga     1380

gcccuagagg ccgaaaugga cggcgcaaaa gggcgacuaa guagcggaca ccuaaaaugc     1440

cggcucaaaa uggacaaacu ccgccucaaa ggcgucuccu acucccuaug caccgcagca     1500

uuuacauuca caaaaauccc cgcagaaacc cuacauggaa ccgugacagu agaaguccaa     1560

uacgcaggaa cggacgggcc augcaaagug cccgcacaaa uggcaguaga caugcaaaca     1620

cucacaccag ucggcagacu aaucaccgcg aaucccguga uaaccgaauc aaccgaaaac     1680

agcaaaauga ugcuagaacu agacccaccc uucggcgaca guuacauagu cauaggcgua     1740

ggagaaaaga aaaucacaca ccacuggcac cgauccggau caacaauagg aaaagcguuc     1800

gaagcgaccg uacgcggagc caaacgaaug gcaguucucg gagacacagc cugggacuuc     1860

ggaagcguag gcggcgcgcu aaacagccuc ggcaaaggca uccaucagau cuucggagca     1920

gcguucaaau cacuauucgg agggaugagc ugguucaguc agauacuaau aggcacauug     1980

uuaguguggc ucggccuaaa cacgaaaaac ggcagcauca gcuuaaccug ccucgcacuc     2040

ggcggcguac ucauuuuucu aagcaccgca guaucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9580
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681082.3 prME-short mRNA Design 3 opt

<400>  9580
gggagaaagc uuaccaugac aucagucggc aucguaggcc uccuucuaac aaccgcaaug       60

gccguagagg uaacgcgucg aggcucagcg uacuacaugu accuggaccg aucagacgcg      120

ggggaggcga uaucguuccc aaccacgcuc ggaaugaaca aaugcuacau acaaauaaug      180

gacuuaggac acaugugcga cgccaccaug uccuacgaau gccccaugcu cgacgaagga      240

guggaaccag acgacgucga cugcuggugc aacacaacca guaccugggu agucuacggc      300

acgugccacc acaagaaagg cgaagcccgc cgcucccgcc gagcugucac acucccaagc      360

cauagcacac gcaaacugca gaccagaagc caaaccuggc ucgaaucuag agaauauaca      420

aaacaccuga uccgcguaga gaacuggaua uucagaaacc caggauucgc ccuagccgca      480

gcagucaucg ccuggcuacu cggauccagc acaucacaga agguaauaua ccucguaaug      540

auauuacuca ucgcaccagc guauucgauc cggugcaucg gcguaagcaa ccgagacuuc      600

guugaaggaa ugucaggcgg gaccuggguc gacgucgucc uggaacacgg aggaugcguu      660

acggucaugg cacaagacaa acccacaguc gacaucgaac ucgucacaac gaccguaagu      720

aacauggcag aaguacgguc cuacugcuac gaagcuagca uaucagacau ggcuucagac      780

uccagaugcc ccacacaggg cgaagccuac cuagacaaac aaucagacac gcaauacguc      840

ugcaaacgaa cccucguaga ccgaggcugg ggaaacggcu gcggccucuu cggcaaagga      900

ucgcucguga ccugcgccaa guuugcaugc uccaagaaaa ugacaggcaa auccauacaa      960

cccgagaacc ucgaauaccg caucaugcuc agcguccacg gaucacaaca cucgggaaug     1020

auagucaacg acaccgggca cgagaccgac gaaaaccgcg ccaaggugga aauaacaccu     1080

aacaguccca gagcagaagc cacacucggc ggauucgggu cacucggccu cgacugcgaa     1140

ccacgaaccg gacuagacuu cagcgaccuc uacuaccuga caaugaacaa caaacacugg     1200

cuuguacaca aagaaugguu ccacgauaua ccacucccau ggcacgccgg ggccgauacc     1260

gguaccccac acuggaacaa caaagaggca cucguagagu ucaaagacgc acacgcaaaa     1320

cgccaaaccg ucgucguacu cgggucacaa gaaggcgccg ugcauacagc ccucgcuggc     1380

gcccuagaag ccgagaugga cggcgcuaag ggucgacuuu ccucaggcca ccuaaaaugc     1440

cgccugaaaa uggacaaacu ccggcucaaa ggggucuccu acucacucug caccgcagcc     1500

uucacuuuca ccaagauacc cgccgaaacc cuccacggca ccgucaccgu cgaaguccaa     1560

uacgcaggaa ccgacggacc cugcaaagua cccgcucaaa uggcgguaga caugcaaacc     1620

cugacgcccg ucggccgccu aauuaccgcc aauccuguca uaaccgaauc gacagaaaac     1680

agcaaaauga ugcucgaacu ggacccaccc uucggcgaca gcuacauagu caucggcguc     1740

ggcgaaaaga aaaucaccca ccacuggcac cgauccggua gcaccauagg aaaagcauuc     1800

gaagccaccg uacggggagc aaaaagaaug gcgguacugg gcgacaccgc augggacuuc     1860

gggagugucg gaggagcccu uaacucguua ggcaaaggca uacaccagau auucggcgcc     1920

gccuucaaaa gccuguucgg cggcaugagc ugguucagcc aaauccuaau cgggacacuc     1980

cuggucuggc uaggccucaa caccaagaac ggauccauua gccuaaccug ccuggcacuc     2040

ggaggaguuc uaauauuccu cagcaccgcc gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9581
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 prME-short mRNA Design 3 opt

<400>  9581
gggagaaagc uuaccaugac gagcguaggc auaguaggcc uccuacuaac caccgcaaug       60

gccguagagg uaacccgccg aggcucagcc uacuacaugu accuagaccg aagcgacgcc      120

ggcgaagcca ucagcuuucc cacgacccua ggagucaaca aaugcuacau acaaauaaug      180

gaccucgggc acaugugcga cgcaaccaug ucauacgagu gcccaaugcu agacgaagga      240

gucgaacccg augacgucga uugcuggugu aacaccaccu caacgugggu cgucuacgga      300

acaugccacc acaagaaagg cgaagcacgg cggagcagac gagcagucac gcuaccaucc      360

cacucaacac gcaaacucca aacccgauca caaacauggc ucgaaucccg cgaauacacc      420

aaacaccuaa ucagaguaga aaacuggauc uuucgcaacc ccgguuucgc acucgcagca      480

gccgcuaucg cauggcuacu aggcucaucc accucacaga aagucauuua ccucgugaug      540

auccuacuaa ucgcacccgc auacucgauc cgcugcauag gaguuagcaa ccgcgacuuc      600

guagaaggaa ugagcggcgg cacaugggua gacguagugc uagaacacgg cggcugcguc      660

accgucaugg cccaggacaa acccacggua gacaucgaau uagucacuac aacggucagc      720

aacauggcag aaguccgcuc auacugcuac gaggcaagca ucuccgacau ggcuuccgac      780

agccgcuguc ccacgcaggg cgaagccuac cuagacaaac aauccgacac acaauacguc      840

ugcaaaagga cccuaguaga cagaggaugg ggaaacgggu gcggacucuu cggaaaaggc      900

agccucguga ccugcgccaa auucgccugc ucaaagaaaa ugaccggaaa aagcauccaa      960

cccgaaaauc ucgaguacag aaucaugcuc agcguccacg gaucacaaca cagcggcaug     1020

auagucaacg acacggggca cgaaacggau gaaaaccgcg ccaaagucga aaucaccccc     1080

aacagccccc gagccgaagc cacccucggc ggguucggau cgcuaggacu cgacugcgaa     1140

ccacgaacag gccuggacuu cagcgaccuc uacuaccuaa cgaugaacaa uaaacacugg     1200

cuaguacaca aagaaugguu ucacgacauc ccacuccccu ggcacgccgg agccgacacc     1260

ggaacacccc acuggaacaa caaagaagca cugguugagu ucaaggacgc acacgcaaaa     1320

cgccagaccg ugguugugcu aggcucacaa gaaggcgcag uacacaccgc ccuggcagga     1380

gcucuagaag ccgagaugga cggagccaaa gggcgccuca gcuccggaca ccucaaaugc     1440

cgccuaaaaa uggacaaacu ccgacuaaaa ggagucucuu acucacucug caccgccgca     1500

uucaccuuca ccaaaauacc cgcagagacc cuccacggca cagugaccgu cgaaguacaa     1560

uacgccggaa cagacggccc augcaaagua ccggcccaaa uggccguaga caugcaaaca     1620

cucaccccag ucggcagacu aaucacagca aaccccguca uaacagaauc cacagagaac     1680

agcaaaauga ugcucgaacu agauccgccc uucggcgaua gcuacauagu aaucgguguc     1740

ggagacaaga agaucaccca ccacugguac cgauccggua gcaccaucgg caaagcauuc     1800

gaagccaccg uccgaggcgc caaaagaaug gcaguccucg gcgacaccgc gugggacuuc     1860

ggcagcguag gaggagcccu caacucccuc ggaaaaggca uccaccaaau cuucggcgcc     1920

gccuucaaaa gccuauucgg uggaaugagc ugguucuccc agauccugau uggcacgcuu     1980

cuaguauggc uaggccuaaa cacgaagaac ggcagcauau cccucacgug ccuugcgcua     2040

ggcggcguac uaauauucuu aagcaccgcc guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9582
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 prME-short mRNA Design 3 opt

<400>  9582
gggagaaagc uuaccaugac cagcgucggc aucgugggcc uccuccuaac uaccgccaug       60

gccgucgaag ucacacgccg cggcagcgcg uauuacaugu accuggacag aagcgaugcc      120

ggaaaagcca uaucauuccc caccacccua ggaguuaaua aaugcuacau acaaaucaug      180

gaccucggcc acaugugcga cgcaaccaug uccuacgaau gcccaaugcu agacgaaggc      240

guagaaccug acgacgugga cugcuggugc aacaccacau caaccugggu cguuuacggc      300

acaugccacc acaagaaagg ggaagcccga cgcuccaggc gcgcggucac ccucccauca      360

cacucaaccc gcaaacuaca aacacgaucc cagacuuggc uagaaucgcg cgaauacacc      420

aaacaccuca ucagagucga gaacuggauc uuccgaaacc ccggcuucgc acuagcagcc      480

gcugccauag caugguuauu aggcucgagc accucacaga aagugauaua ucucgucaug      540

auacugcuca ucgcgccagc guacuccauu cggugcauag gcguaagcaa cagagacuuc      600

gucgaaggca uguccggcgg aaccugggua gacgucgucc ucgaacacgg uggcugcgug      660

accguaaugg cccaagacaa acccacagua gacaucgaac ugguuaccac aaccguaucc      720

aacauggccg aagugcgcuc auauugcuau gaagccagca ucucagacau ggcaucggac      780

ucccgcugcc caacacaagg agaagcguac cucgacaaac aaagcgacac ccaguacguc      840

ugcaaaagga cacucgucga cagaggcugg ggcaacggau gcggccucuu cggcaaaggc      900

agccucguaa ccugcgccaa auuugccugu agcaagaaaa ugaccggcaa aagcauacaa      960

cccgaaaacc ucgaauaccg gauuaugcuc agcguccacg gcucacaaca cagcggcaug     1020

aucgucaaug acauaggcca cgagaccgac gaaaaccgcg ccaaagucga gaucaccccc     1080

aacuccccac gcgcagaggc aacucucggg ggauucggcu cccucggcuu agacugcgaa     1140

ccccgaaccg gacucgacuu cucagaccug uacuaccuca ccaugaauaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacaua ccccuccccu ggcacgcagg cgcggacacc     1260

ggaacaccgc acuggaacaa caaagaggcc cucgucgagu ucaaagacgc acacgccaaa     1320

cgucaaacag uagucguacu cggcucccaa gaaggcgccg uacauaccgc acucgccggc     1380

gcccuagaag cagaaaugga cggcgccaaa ggacgucuaa gcuccggcca ccucaaaugc     1440

agacugaaaa uggauaaacu acgccucaaa ggcgucagcu acucccucug cacagccgcu     1500

uucacauuua ccaaaauccc agccgaaaca uuacacggaa cuguuaccgu agaaguccag     1560

uacgccggca ccgacggccc augcaaaguc ccagcccaaa uggcagucga uaugcaaaca     1620

cuuacaccag ucggacgccu caucacagca aacccgguaa uaaccgaauc aaccgaaaac     1680

uccaaaauga ugcuagagcu cgaucccccu uuuggcgaca gcuacaucgu caucggaguc     1740

ggggacaaga aaaucaccca ccacuggcac cgcucaggcu ccaccaucgg gaaagcauuc     1800

gaagccacag uacguggugc caaacgcaug gccguacucg gcgacacagc gugggacuuc     1860

ggcagcgucg gaggcgcacu caacucacuc gggaaaggca uccaccaaau cuucggcgcc     1920

gcauucaaau cccuauucgg cggcauguca ugguucuccc aaauacuaau cggcacccua     1980

cucgucuggc uaggacucaa caccaaaaac ggaagcauca gccucacgug ccuagcccuc     2040

ggaggcgucc ucauauuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9583
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 prME-short mRNA Design 3 opt

<400>  9583
gggagaaagc uuaccaugac aagcaucggc aucgucgggc uccuacucac caccgcaaug       60

gccgcagaaa ucacccgaag aggaagcgcc uacuacaugu accuggaccg gagcgacgca      120

ggaaaagcca uuagcuucgc caccacacua ggaguaaaca aaugucacgu gcagaucaug      180

gaccuaggcc acauguguga cgcgacaaug uccuacgaau gcccaaugcu ggaugaagga      240

guagaacccg acgacgugga cugcuggugc aacacaacua gcacaugggu cgucuacggc      300

acaugccacc acaagaaagg agaggcccga agaucccggc gcgcagucac ccuaccgucc      360

cacaguaccc gcaaacucca aaccagaagc caaacauggc uagaaagcag agaauacacg      420

aaacaccuca ucaaaguuga aaacuggauc uuccgaaacc ccggauucac gcugguagca      480

gucgcaaucg cauggcuccu agggucuagc acuucacaaa aaguaauaua ccucgucaug      540

auccuucuga uagcgccggc cuacagcaua cgcugcaucg gaguauccaa uagagacuuc      600

guagagggga uguccggcgg cacauggguc gacguagucc ucgaacaugg aggcuguguc      660

accguaaugg cccaggacaa gccaaccgua gacaucgagc ucguaacaac caccguaucc      720

aacauggcgg aaguaagaag cuacugcuac gaagccagca ucagcgacau ggccuccgac      780

ucgcggugcc caacccaagg cgaagccuau cucgacaagc aauccgacac acaguacgua      840

ugcaaaagaa cccucgucga ucgcggcugg ggcaacgggu gcggccucuu cggaaaaggc      900

agccucguaa ccugcgcaaa auuuaccugc uccaaaaaga ugaccggaaa aucaauccag      960

ccagaaaacc ucgaauaccg caucaugcuc agcguccacg gaagccaaca uagcggcaug     1020

aucguaaacg acacaggaca cgaaaccgac gaaaaccgcg caaagguaga aguaaccccc     1080

aacagcccac gcgcggaagc gacgcuagga ggcuucggaa gccugggacu cgacugcgaa     1140

ccccgcacgg gccuggacuu cucagaccuc uacuaccuca caaugaacaa caagcacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccacucccgu ggcacgcggg cgcagacacc     1260

ggaacacccc acuggaacaa caaagaggcc cuaguggagu ucaaagacgc acacgccaaa     1320

cgacaaaccg uagucguccu aggcagccaa gaaggcgccg uccacaccgc ccucgcaggc     1380

gcccuagaag ccgaaaugga cggagcaaaa ggacgacuau ucucgggaca ccucaaaugc     1440

agacucaaaa uggacaaacu uagacuaaaa ggggucucau acagccucug uacagcagca     1500

uucaccuuca cgaaaguacc ggccgaaaca cuacacggaa ccguaacagu agagguacaa     1560

uacgcaggga cggacggccc gugcaaaguc ccagcccaaa uggcuguaga caugcaaacc     1620

cuaacaccag ucggccgccu cauaaccgca aacccaguua ucacagaauc aacagaaaac     1680

ucaaaaauga ugcuagaacu agacccaccc uucggagaca gcuacauagu cauaggaguu     1740

ggagacaaga aaauaacaca ccacuggcac cggucaggga gcacaaucgg aaaagccuuc     1800

gaagcaaccg uaagaggcgc aaaaagaaug gcgguccuug gcgacaccgc augggacuuc     1860

ggaucaguug gaggcguguu caacucacuc gggaaaggca uacaccaaau auucggagcg     1920

gcauucaaau cacuauucgg gggaaugucc ugguucaguc aaauacucau aggcacccug     1980

cuaguauggc uugggcuaaa cacaaagaac ggauccauaa gccuaaccug ccucgcacug     2040

ggaggcguca ugaucuuccu aucaaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9584
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 prME-short mRNA Design 3 opt

<400>  9584
gggagaaagc uuaccaugac aucaauaggg aucauaggcc uccuccugac aaccgcaaug       60

gcggcugaga uaacgcgccg agggagcgcc uacuacaugu accuagacag aucggacgca      120

gggaaagcaa ucagcuucgc caccaacuug ggagucaaca aaugccacgu ccaaaucaug      180

gaccucggac acaugugcga cgccacaaug ucuuacgagu gccccaugcu agacgaaggg      240

guagaacccg acgacgugga cuguuggugc aacaccacaa gcaccugggu agucuacgga      300

accugccacc acaagaaagg cgaagcccgg agaucccgcc gagccguaac ccuccccucc      360

cacucgaccc gaaaacucca gacaagaagu caaaccuggc uugaauccag agaauacacg      420

aaacaccuaa uaaaaguuga aaacuggauc uuccgcaacc ccggcuucgc ccucgccgcg      480

gucgccauag ccuggcuccu aggcagcucg accagucaaa aaguaaucua ccuagucaug      540

auccuacuca ucgccccggc auacagcaua cgauguaucg gagugagcaa ccgagacuuc      600

gucgaaggaa ugagcggcgg aacuugggua gacguugucu uagagcacgg cggcugcgua      660

accgucaugg cccaggacaa accaaccguc gacaucgagc uagugaccac cacggucuca      720

aacauggccg aagugagauc guacugcuac gaagccucca uauccgacau ggcaucagac      780

ucgagaugcc caacacaagg cgaagccuac cucgacaaac aaagcgacac acaauacgug      840

ugcaaacgca cccuaguaga cagaggaugg ggcaacggau gcggacucuu cggaaaaggc      900

agccuaguca cuugcgcgaa auucacaugc agcaagaaaa ugaccggcaa aagcauacaa      960

ccggaaaacc uagaauaccg caucaugcuc agcguacacg guucacaaca cuccggcaug     1020

aucgucaacg acauaggaca cgaaaccgac gaaaaccgcg caaaagugga agucacccca     1080

aacucaccga gagcagaagc aacacucggc ggauucggau cacuaggucu cgacugcgaa     1140

ccgcgcacug gccuagacuu cuccgaccuc uacuaccuua caaugaacaa caagcacugg     1200

cuaguccaca aagaaugguu ccaugacauu ccguugccau ggcacgcagg agccgacaca     1260

ggaacaccac acuggaacaa caaagaagcg cuaguagagu ucaaagacgc ccacgccaag     1320

cgucaaaccg ugguaguccu aggaagccaa gaaggcgccg ugcacacagc acuagccgga     1380

gcacucgaag ccgaaaugga cggugccaaa gggcgacuau ucagcggcca ccucaaaugc     1440

aggcucaaaa uggacaaacu gcgacuaaaa ggaguaucgu acucacuaug caccgccgcc     1500

uucacauuca caaaaguacc ggccgaaacc cuacacggca caguaaccgu ggaaguccag     1560

uacgcaggaa ccgacggacc uugcaaaguc cccgcccaaa uggccgucga caugcaaaca     1620

cucacacccg ucggcagacu gauaaccgca aacccaguaa ucaccgaaag cacugagaac     1680

agcaaaauga uguuagaacu agauccccca uucggcgacu cauacaucgu caucggaguc     1740

ggcgacaaga aaauaaccca ccacuggcac cgcaguggcu caaccaucgg caaagcauuc     1800

gaagccaccg uacgcggcgc caaacgcaug gcaguccucg gugacacagc augggauuuc     1860

ggaagcgucg gcggcgucuu caacucccuc ggcaaaggug ugcaccaaau cuucggcgcc     1920

gccuucaaga gccucuucgg aggcaugucg ugguucucac aaauccuuau cgggacacuc     1980

cuagucuggc uaggacuaaa caccaagaac ggcaguauca gccuaaccug ccucgcccua     2040

ggcgggguaa ugauauuccu aagcacggcc guaagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9585
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383121.1 prME-short mRNA Design 3 opt

<400>  9585
gggagaaagc uuaccaugac cuccaucggg aucauaggcc uauuacuaac caccgcaaug       60

gcagccgaga ucacccggcg cgguagcgcc uacuacaugu accucgauag aucagaugca      120

ggcaaagcaa uauccuucgc aaccacacuc ggaguaaaca agugccacgu ccaaaucaug      180

gaccuaggcc acaugugcga cgcaacaaug ucauacgaau gcccaaugcu agacgagggc      240

gucgaacccg acgacguaga cugcuggugc aacaccacaa gcacaugggu agucuacgga      300

acaugucacc acaagaaagg agaggccagg agaagccgac gcgcagucac ccucccaagc      360

cacucgacac gaaaacucca aacacgcagc caaacauggc uggaaagccg cgaauacacc      420

aaacaccuca ucaaaguaga aaacuggauc uuccgcaacc caggcuucgc acucguagcc      480

guagccauag ccuggcuccu gggaagcagc accagccaaa aagucauaua ccucguaaug      540

auccuccuga ucgcaccagc cuacucgauc aggugcauug gcgucagcaa ccgagacuuc      600

gucgaaggca uguccggagg cacaugggua gacgucgugc uagagcacgg cggcugcguc      660

acggucaugg cccaagacaa acccacggug gacauugaac ucguaaccac cacagucagc      720

aacauggcug aagucagaag cuacugcuac gaggcaucga ucagcgacau ggccagugac      780

ucaagaugcc caacacaagg ggaagcauac cucgacaaac agucagacac ccaauacguc      840

ugcaagcgaa cacucguaga ccgcggcugg ggcaacggcu gcggacucuu cggcaagggc      900

ucucugguca caugcgccaa auucacgugu agcaagaaaa ugacuggcaa aucaauccaa      960

ccagaaaauc ucgaauaccg aaucaugcua uccguacacg gcucacaaca cagcggcaug     1020

auaguaaaug acauagguca ugaaacugac gaaaacagag caaaagucga gguaaccccc     1080

aacagcccgc gcgcugaagc aacgcucggc ggcuucggga gccuaggacu cgacugcgaa     1140

ccccgaacgg gccuagacuu uagcgaucug uacuaccuca caaugaacaa caaacacugg     1200

cucgugcaca aagagugguu ccacgacauc ccccuccccu ggcacgccgg agccgacacc     1260

ggcacacccc auuggaacaa caaagaagcc cucguggagu ucaaggacgc acacgcaaaa     1320

cggcaaaccg uagucguccu aggaucacag gaaggcgccg uccacaccgc cuuagcggga     1380

gcacucgaag cagaaaugga cggcgccaaa gggcgccuau ucuccggaca ccugaagugu     1440

cgucucaaaa uggacaaacu ccgacuaaaa ggaguguccu acagccucug caccgcagca     1500

uucacauuca ccaaaguccc cgccgaaacc cuccacggga caguaacagu cgaaguccaa     1560

uacgccggua cagacggacc augcaaaguc cccgcccaaa uggcaguaga caugcaaaca     1620

cuaacccccg ucgggcgacu caucaccgcc aaccccguua uaacagaaag cacggaaaac     1680

agcaaaauga ugcuggaacu ugacccuccc uucggggacu cauacauagu aauaggcguc     1740

ggagacaaga aaaucacaca ccauuggcac cgaagcggau ccacaauagg aaaagcauuc     1800

gaagccaccg uacgaggggc aaaacggaug gcaguacuag gcgacaccgc cugggacuuu     1860

ggcucaguag gcggcgucuu caacagccuc gggaaaggca uccaccaaau auucggugcc     1920

gccuucaaau cacucuucgg cggaaugagc ugguucagcc aaauccugau uggaacccua     1980

cucgucuggc ugggcuuaaa caccaaaaac ggcagcaucu cccugacaug ucucgcccua     2040

gggggaguaa ugauauuccu aucaaccgcc gucagcgcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9586
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268949.1 prME-short mRNA Design 3 opt

<400>  9586
gggagaaagc uuaccaugac aagcauaggu aucauagggc uucuacucac aaccgccaug       60

gcagccgaaa ucacccgacg gggcuccgcc uacuacaugu acuuagacag aagcgacgca      120

ggaaaagcca ucucauucgc caccaaccua ggagucaaca agugccacgu acaaauaaug      180

gaccugggac acaugugcga cgccacuaug uccuacgaau gccccaugcu cgacgagggc      240

gucgaaccag acgacguuga cugcuggugc aacaccacaa gcacaugggu cgucuacggg      300

accugccacc acaagaaagg cgaagcacgc cggagccggc gcgccgucac gcuuccgagc      360

cacagcaccc gcaaacuaca aacaagauca cagacguggc ucgagagcag agaauacacc      420

aagcaccuca ucaaaguaga aaacuggauu uuccgcaacc ccggauucgc ucuagccgca      480

gucgcaaucg ccuggcuccu aggcucgagc acaagccaaa aagucaucua ccuaguaaug      540

auccuccuaa ucgcacccgc auacucaaua cgcugcauag gcgucagcaa ccgcgacuuu      600

guagaaggaa ugucaggagg gacaugggua gacguagucc ucgaacacgg aggcugcguc      660

accgucaugg cacaggacaa acccaccgua gacauagagc ucguaaccac aacaguaagc      720

aauauggcgg aaguccguuc cuacuguuac gaggcuucaa ucucagacau ggcgucagac      780

ucacgauguc ccacccaagg cgaagcauac cuggacaaac aaucagacac ccaauacgua      840

uguaaaagga cccucguuga ccgcggcugg gggaacggau gcgggcuauu cggaaaagga      900

ucccucguca ccugugccaa auucacaugc ucaaagaaaa ugaccggaaa aucaauccag      960

ccggaaaacc uagaauacag gauaaugcuc ucaguccacg gaagccaaca cagcggaaug     1020

auagucaacg acgaaaacag ggccaaaguc gagguuacac ccaacagccc acgcgccgaa     1080

gcaacacuag gaggauucgg gagccuaggu cuugacugcg aaccgcgaac cggccuggac     1140

uucucagacc ucuacuaccu aaccaugaac aacaaacacu ggcucguaca caaagaaugg     1200

uuccacgaca ucccgcuccc guggcacgcc ggagcagaca caggcacccc acacuggaac     1260

aacaaagagg cccucguaga guucaaagac gcccacgcga aacgccaaac aguaguagua     1320

cuggguuccc aagaaggcgc aguccacacc gcgcuagcag gcgcacuaga agccgaaaug     1380

gacggagcca aaggacgucu auucuccgga caccucaaau gccggcuaaa aauggacaaa     1440

cuaagacuca aaggaguauc guacucccuc ugcacagccg ccuuuacauu cacgaagguc     1500

ccagccgaaa cccuacacgg aaccgugacc gucgaaguac aauaugcggg aaccgacggc     1560

ccaugcaagg uaccagccca aauggcaguc gacaugcaaa cccucacgcc cgucggacgc     1620

cucaucacag caaauccagu aaucacagaa agcaccgaaa auucaaaaau gaugcuggaa     1680

cuagacccac ccuucggaga cagcuacauc guaaucggcg ucggagacaa gaaaaucaca     1740

caucacuggc auaggagcgg gagcacaauu ggaaaagccu ucgaagcaac cguaagaggc     1800

gccaaacgaa uggcgguccu aggugacaca gccugggacu ucggcagcgu cgggggcguc     1860

uucaacagcc uaggcaaagg cauccaccaa auauucggag cagccuuuaa aagccuauuc     1920

gguggcaugu caugguucag ccagauccuc auaggaaccc ugcuagucug gcuaggacua     1980

aacacgaaaa acgggagcau aagccugaca ugccucgcuc ugggaggcgu caugauauuc     2040

uuaagcacgg ccguaagcgc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  9587
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU955595.1 prME-short mRNA Design 3 opt

<400>  9587
gggagaaagc uuaccaugac cagcauagga auaguaggcc uccuacucac aaccgccaug       60

gcggcggaga ucaccagacg aggcagcgca uacuacaugu accucgacag gucagacgcc      120

gggaaagcaa ucagcuucgc aaccacacug ggagucaaca aaugccacgu acagauaaug      180

gaccugggac acauguguga cgcaaccaug ucauacgagu gccccaugcu agaugaagga      240

guagagcccg acgacguaga cugcuggugc aauacaacca gcacaugggu cguguacgga      300

accugccacc acaagaaagg cgaagcuaga agaagccgca gagcgguaac ccuaccaagc      360

cacagcacgc gaaaacucca aacccgaagc caaaccuggc uugagagccg ggaauacacg      420

aaacaccuaa ucaagguaga aaacuggaua uuccgcaauc ccgguuucgc gcucgucgca      480

gucgcaauag cauggcuccu aggaagcagc acaagccaga aagugauaua ccuaguaaug      540

auccuacuga uagccccagc guacagcauc cgcugcaucg gcguaagcaa ccgcgacuuc      600

guagaaggaa uguccggagg cacaugggua gacguagucc ucgagcacgg cggcugugua      660

acaguaaugg cacaagacaa accaacgguc gacauagagc uagucaccac aacaguaagc      720

aacauggcag aagugcgaag cuacugcuac gaagcaucca ucucagacau ggccagcgac      780

agucgaugcc ccacacaagg cgaagcauac cuagacaaac aauccgacac ccaauacguc      840

ugcaaaagaa cgcuagucga uagagguugg ggcaacggcu gcggauuauu cggcaaaggc      900

ucgcuaguga caugcgcgaa auucacaugc agcaagaaaa ugacgggcaa aagcauccag      960

cccgaaaacc ucgaguacag aauaaugcua uccguccacg gcagccaaca cagcggcaug     1020

auagucaacg acacugggca cgaaacagac gaaaacaggg ccaaaguaga agucacccca     1080

aacagcccac gcgcggaagc gacccucgga ggguucgggu cacucggacu agacugcgaa     1140

ccacgaacag ggcucgacuu cuccgaccuc uacuaccuaa cgaugaacaa caaacacugg     1200

cugguacaca aagagugguu ccacgacaua cccuugccau ggcacgcagg ggcggacaca     1260

ggaaccccac acuggaacaa uaaagaggcg cuagucgagu ucaaagacgc acacgcaaaa     1320

cgacaaacag uaguaguacu aggaagccaa gaaggcgcag uacacacagc acuagccggc     1380

gcgcuggaag ccgaaaugga cggcgcaaaa ggacgacuau ucagcggcca ccugaaaugc     1440

aggcucaaaa uggacaaacu gagacugaaa ggagucuccu acucacuaug cacagcagca     1500

uuuaccuuca cgaaaguacc agccgaaacc cuacacggaa caguaacggu agaaguccag     1560

uacgcaggaa cagacgggcc augcaaagua ccagcacaga uggccgucga caugcagacc     1620

cucacaccag ucgggcgccu aauuacagcc aacccgguaa ucacagaaag caccgaaaac     1680

uccaagauga ugcuagaacu agacccaccc uucggcgaca gcuacaucgu gaucggugua     1740

ggugacaaga aaaucacaca ccacuggcac cggagcggca gcacaauagg gaaagccuuc     1800

gaagcgacag uacgcggcgc gaaacgcaug gccguacucg gcgacaccgc augggacuuc     1860

ggaagcgugg gcggcgucuu caacagccuc ggaaaaggua uccaccaaau cuucggggca     1920

gccuucaaaa gccucuucgg gggaaugagc ugguucucac aaauacuuau agggacacug     1980

cuaguauggc ucggccucaa caccaaaaac ggaagcauaa gccuaaccug ccuagcacug     2040

ggaggcguga ugauauucuu aagcacagcc gucucggcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9588
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 prME-short mRNA Design 3 opt

<400>  9588
gggagaaagc uuaccaugac gagcauagga aucgucggac uacuacuaac caccgcaaug       60

gccgcagaaa ucacacgcag aggaagcgca uacuacaugu accuagauag gagcgaugcc      120

ggcaaagcca ucagcuucgc gacaacacua ggugucaaca aaugccacgu acaaauaaug      180

gaccuaggcc auaugugcga cgccaccaug ucauacgaau gccccaugcu agacgaaggc      240

gucgaaccag acgacgugga cugcuggugu aauaccacau ccaccugggu agucuacggc      300

acaugccagc acaagaaagg agaggcacga cgcucaagac gagcgguaac acuacccagc      360

cacucaaccc gcaaacugca aacgcgcuca caaacauggc uagaaucaag ggaauacaca      420

aaacaccuaa uaaaagucga aaacuggaua uuccgcaacc ccggcuucgc acuggucgca      480

guagcaauag cguggcuccu aggcucgagu accucccaaa aagucauaua ccucgucaug      540

auccuccuca uagccccagc auacuccauc cgcugcaucg gcgucagcaa cagagacuuc      600

guagaaggca ugucuggagg cacaugggug gacguggugc uagaacacgg aggcugcguc      660

accguaaugg cccaagacaa acccacaguc gacaucgaac ucgucacaac cacgguuagc      720

aacauggcag agguacgcuc cuacugcuau gaagccagca uaucagacau ggcaagcgac      780

ucgcgcugcc ccacccaagg cgaagccuac cucgacaaac aaagugacac gcaauacgua      840

ugcaaaagaa cccuagucga ccgaggcugg ggaaacgguu gcggccucuu cggaaaaggc      900

agccuaguaa caugcgccaa auucacaugc uccaagaaaa ugacagggaa aagcauacaa      960

cccgaaaacc uggaauacag aaucaugcuu agcguacacg gcagccaaca uucagguaug     1020

auaguaaacg acaucggcca cgaaaccgau gagaaccgcg ccaaaguaga agucaccccc     1080

aauuccccgc gcgccgaagc aacccuaggc ggauucggau cacucggccu ugacugcgaa     1140

ccccgcacag gacucgacuu cagcgaccuc uacuaccuaa caaugaacaa caaacacugg     1200

cucguacaca aagagugguu ucacgacauc ccccuacccu ggcacgccgg agccgacacc     1260

ggaacacccc acuggaacaa caaagaagcc cuaguggagu ucaaagacgc acacgccaaa     1320

cgccaaaccg uagucguccu aggcagccaa gaaggcgccg uccacacugc guuagcagga     1380

gcauuggaag ccgaaaugga cggggccaaa ggucggcuau ucagcggcca ccugaaaugc     1440

cgccucaaaa uggacaaacu ucgccuaaaa ggcgucuccu auucacucug caccgccgua     1500

ugcacagcag ccaaaguacc ggcggaaacc cuacacggca ccguaacggu cgaaguacaa     1560

uacgcaggaa cagacggucc augcaaagua cccgcucaaa uggccguaga caugcaaacc     1620

cucacccccg uaggcagacu aaucaccgcc aacccaguaa uaaccgaaag cacggaaaac     1680

uccaaaauga ugcuagaacu agacccgccc uucggcgaca gcuacaucgu cauuggagua     1740

ggggacaaga aaaucacaca ccacuggcac agauccggau ccacaauagg caaagcguuc     1800

gaagcaaccg uccgcggcgc aaaacgcaug gccguccucg gcgacacggc gugggacuuc     1860

gguucaguag gcggagucuu caacucccua ggcaaaggaa uacaccaaau cuucggcgcc     1920

gcauucaaau cgcuguucgg cggcaugucg ugguucucac aaauacucau cgggacccuc     1980

cuaguauggc ugggacucaa uaccaaaaac ggaucaaucu cacucacaug ccuggcgcug     2040

ggcggcguaa ugauauuccu aucaacagca gucucggccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9589
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601166.1 prME-short mRNA Design 3 opt

<400>  9589
gggagaaagc uuaccaugac uagcaucggc aucgucggcc uccuacugac cacagcuaug       60

gcagcagaaa ucacccgacg gggguccgca uacuacaugu accuagaccg cucagacgcc      120

ggaaaagcga ucuccuucgc aacaacgcua ggagucaaca aaugccacgu ccaaaucaug      180

gaccuaggcc acaugugcga cgcuacaaug agcuacgaau gccccaugcu agaugagggc      240

guagaacccg acgacgucga cugcuggugc aacaccacua gcacgugggu aguauauggc      300

acaugccacc acaaaaaggg agaagcacgg cggagccgac gcgcaguaac acucccaagc      360

cacagcacac gaaaacucca aacccguagc caaacguggc ucgaauccag agaauacacc      420

aaacaccuaa ucaaaguaga aaacuggauc uuccgaaacc caggcuucgc ccucguagca      480

gucgcaauag cauggcuccu aggauccagu acaagccaga aaguaaucua ccuaguaaug      540

auccuccuaa uagcacccgc cuacuccauc agaugcauag gcguaagcaa ccgagacuuu      600

guggaaggaa uguccggcgg aacaugggua gacgucgugc uggaacacgg aggcugcgua      660

acgguaaugg cgcaagacaa acccaccgua gacaucgaac uggucaccac caccguauca      720

aacauggcag aaguacgcuc cuacugcuac gaagcgucaa uaucagacau ggccucggac      780

ucccggugcc caacccaagg cgaggcauac cuagacaaac aauccgacac ccaauacguc      840

ugcaaacgaa cccugguuga ucgaggaugg gguaacggcu gcggacucuu cgguaaaggc      900

agucuaguca ccugugcaaa auucacaugc ucaaagaaaa ugaccggaaa aucaauucaa      960

ccagaaaacc ucgaauaucg aauaaugcuc ucaguacacg gaagccaaca cucaggcaug     1020

auagucaaug acgaaaaccg cgccaaaguu gaagucaccc caaacagucc cagagcagaa     1080

gccacgcucg gcggguucgg gagccuagga cuggacugug aaccccggac gggccuggac     1140

uucagcgacc uauacuaccu caccaugaac aacaaacacu ggcuaguaca caaagaaugg     1200

uuucacgaua uaccccuccc guggcacgcc ggcgccgaua ccggcacucc ccacuggaac     1260

aacaaagaag cacuggucga guucaaagac gcacacgcaa aacgccaaac ugucguaguc     1320

cucggguccc aagaaggcgc cguacacaca gcacucgccg gcgcgcucga ggcagaaaug     1380

gacggcgcca aagguagacu cuucucagga caccuaaagu gccgccuuaa aauggacaaa     1440

cuccgccuca aaggaguguc guacuccuua ugcaccgccg cauucaccuu cacaaaagua     1500

ccagccgaga cacuccacgg caccguaacc guagaaguac aauacgccgg gaccgacgga     1560

ccaugcaagg uacccgcaca aauggccgua gacaugcaaa cacucacgcc agucggacgc     1620

cucaucaccg caaaucccgu caucacagag uccaccgaga acuccaaaau gaugcucgaa     1680

cucgaucccc cauucggcga cuccuacaua gucaucggcg ucggagacaa gaaaaucacc     1740

caccacuggc accgauccgg cucgaccauc ggcaaagccu ucgaagccac cguaagaggc     1800

gccaaacgga uggccguccu gggcgacacg gccugggacu ucggauccgu cgguggcguu     1860

uucaacucac ucggaaaagg aauccaccaa aucuucggag ccgccuucaa aucacuauuc     1920

ggaggcaugu ccugguucag ccaaauccuc aucggaaccc uacucgucug gcuuggucuc     1980

aacaccaaaa acggcagcau cagcuuaaca ugccucgcac ucggaggcgu aaugaucuuu     2040

cucuccaccg ccgucagcgc augaggacua guuauaagac ugacuagccc gaugggccuc     2100

ccaacgggcc cuccuccccu ccuugcaccg agauuaauag aucuaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  9590
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 prME-short mRNA Design 3 opt

<400>  9590
gggagaaagc uuaccaugac aagcauuggc auuauaggcc uacuucucac cacugccaug       60

gccgccgaaa uaacacgacg aggaagcgcc uacuacaugu accuagacag aucggacgca      120

ggcaaagcaa ucagcuucgc aacaacccuc ggagucaaca aaugccacgu acaaaucaug      180

gaccuaggac auaugugcga cgcaacaaug ucauacgaau gccccaugcu ggacgaaggc      240

gucgaacccg acgacguaga cugcuggugc aacaccacaa guaccugggu agucuacgga      300

accugccacc acaaaaaggg agaaacccga cggucccgca gaucaguauc ccuccgcuac      360

cacuacacaa gaaaacucca aacccgaagc caaaccuggc ucgaaucccg cgaauacaaa      420

aaacaccuaa ucaugguaga aaacuggaua uuccggaacc caggauucgc caucgugagu      480

guagccauca cauggcucau gggcagucug accagccaaa aagucaucua ccucguaaug      540

aucgugcuaa uaguacccgc cuacagcauc ucaugcaucg gcgucucaaa ccgcgaccuc      600

guagaaggaa uguccggagg caccuggguc gacgugguuc ucgaacaugg aggaugcgug      660

acggaaaugg cccaagacaa accaaccguc gacaucgaac ucguaacgau gaccgucagc      720

aacauggcag aagucagauc cuacugcuau gaagcguccc ucagcgacau ggcaagcgcc      780

agccgaugcc ccacacaggg cgagcccuca cucgacaaac aaucagacac acaaagcguc      840

uguaaaagaa cccucgggga ccgaggcugg ggcaacgggu gcggcauauu cggaaaaggu      900

ucgcuaguaa caugcucaaa auucaccugc ugcaaaaaaa ugcccggcaa aucgauacaa      960

ccagagaacc ucgaauacag aauaaugcua cccguacacg ggagccaaca cagcggcaug     1020

auaguaaacg acauaggaca cgagacagac gagaacagag caaaaguaga agucacgcca     1080

aauuccccgc gagccgaagc aacacucgga ggcuucggaa gccuagggcu cgacugcgaa     1140

ccacgcaccg gccucgacuu cucagaccua uacuaccuga ccaugaacaa caaacacugg     1200

cuuguccaca aagaaugguu ccacgauauc ccucuacccu ggcacgccgg agcagacacc     1260

ggcacacccc acuggaacaa caaagaggcc cugguagagu ucaaagacgc acacgcaaaa     1320

cgucaaaccg ucguggucuu aggcagccaa gaaggcgccg uacacaccgc acucgccggc     1380

gcccucgaag cggagaugga cggggcuaaa ggacgccugu ucucaggcca ucucaaaugc     1440

cgucuaaaaa uggacaaacu aagacuaaaa ggaguaucgu acagccucug caccgccgcc     1500

uucacauuca ccaaaguacc ggcagaaaca cugcacggaa cagugacagu ggaaguccaa     1560

agcgccggaa cagauggacc cugcaaaguc cccgcccaga uggccguaga caugcaaaca     1620

cucacacccg uaggccggcu aaucacagca aacccgguaa ucaccgaguc aacagaaaac     1680

uccaaaauga ugcuggaacu ggauccgcca uucggagacu cauacauagu cauaggugua     1740

ggagacaaga aaauaaccca ccacuggcac cgaagcggca gcaccauagg aaaagccuuc     1800

gaagccacgg uacgcggagc caaaagaaug gccgugcucg gagacaccgc cugggacuuc     1860

ggcucaguag ggggcguauu caacagccua ggaaaaggca uacaccaaau cuucggagca     1920

gcauuuaaaa gccuauucgg aggaaugagc ugguucucac aaauacuaau cggaacccua     1980

cuaguguggc uaggacucaa caccaaaaac ggcagcaucu cacucacgug ccuagcccug     2040

ggaggaguca ugauauuccu cuccacagcc guauccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9591
<211>  2287
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447520.1 prME-short mRNA Design 3 opt

<400>  9591
gggagaaagc uuaccaugag cguaggcaua gucggccuac uccugacgac cgcaauggcc       60

gcagaaguga cacggcgggg cagcgccuac uacauguauc uggacagaaa cgacgcgggg      120

gaagccaucu cauucccaac gacccuaggc augaacaaau gcuacauaca aauaauggac      180

cuagggcaca ugugcgacgc aacgaugucc uacgagugcc cgaugcucga cgaaggugua      240

gaaccagacg acgucgauug cuggugcaac accacaucca cauggguagu auacggcacu      300

ugccaccaua agaaagggga agcacgccgc agccgacgcg ccguaacacu cccuagccac      360

uccacgcgca aacuacaaac aagaagccaa accuggcuag aaucuagaga auacacgaaa      420

caccugauaa gaguagaaaa cuggaucuuc cgaaacccgg gauucgcccu cgccgcagcc      480

gccauagcau ggcuccucgg aagcaguacc agucaaaaag uaaucuaccu gguaaugauc      540

cuccuaaucg cacccgcgua cagcaucagg ugcaucggcg ugagcaaccg cgacuucgua      600

gaagggauga gcggcggaac augggucgac gucguccuag agcacggagg augcgucaca      660

guaauggcac aagacaaacc uacgguagac aucgagcucg ucaccaccac cguaaguaac      720

auggccgaag uacggucaua uugcuacgaa gcauccauaa gcgacauggc cagcgacucc      780

cgauguccaa cucaaggaga agcauaccua gacaaacaau cggacacaca auacguaugc      840

aaaagaaccc ucguagacag aggauggggc aacggaugcg gacuauucgg aaaaggcucg      900

cuaguaaccu gcgccaaauu cgcgugcucc aagaaaauga caggaaaaag cauucaacca      960

gaaaaccuag aauacagaau aaugcugucc guacacggaa gccaacauag cggaaugauc     1020

gucaacgaca ccggacacga aacggacgag aacagagcaa aaguagaaau caccccaaac     1080

aguccccgcg cagaggcgac gcuaggaggu uucggaagcc uaggacugga cugcgaaccc     1140

cgcacgggcc ucgacuucag cgaccuguac uaccucacca ugaacaacaa acacuggcua     1200

gugcacaaag aaugguuuca cgacaucccc cuaccguggc acgcaggcgc agacacagga     1260

acaccgcacu ggaacaacaa agaagcacug gucgaguuca aagacgccca cgcaaaaaga     1320

cagacagugg ugguccuggg cagccaagag ggagccguac acacagcgcu cgcgggagca     1380

cuggaagccg agauggacgg cgcaaaagga cgccuguccu caggacaccu caaaugccga     1440

cuaaaaaugg acaaacucag acucaaagga gucagcuacu cacuuuguac agccgccuuc     1500

acauucacaa aaauaccagc cgaaacacua cacggaacag uaaccgucga aguacaguac     1560

gcaggaacag acggaccgug caaagucccc gcgcaaaugg caguagacau gcaaacccug     1620

accccagucg gacgacuaau aaccgcaaac cccguaauca cagaauccac cgaaaauucc     1680

aaaaugaugc ucgagcuaga cccacccuuc ggcgacagcu acauagugau cggcgucggg     1740

gaaaagaaaa uaacccacca cuggcaccga agugggagua caauaggcaa agcauucgag     1800

gcaaccguca gaggggccaa acgaauggca guacugggag acacagcaug ggacuucgga     1860

ucagugggcg gagcccuaaa cucacucgga aaaggaaucc aucaaaucuu cggggccgcg     1920

uucaaaucac ucuucggugg caugagcugg uucagccaaa uacucauagg cacacuccua     1980

auguggcucg gacugaacac gaaaaacgga agcauaagcc ugaugugccu ggcacuaggc     2040

ggcguccuca uauuuuuaag uacagccgua agcgccugag gacuaguuau aagacugacu     2100

agcccgaugg gccucccaac gggcccuccu ccccuccuug caccgagauu aauagaucua     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2280

cagaauu                                                               2287


<210>  9592
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 prME-short mRNA Design 3 opt

<400>  9592
gggagaaagc uuaccaugac cuccgugggg auagucggac uacuccugac aaccgccaug       60

gccgccgaag uuacccgacg aggcagcgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcaa ucuccuuccc caccacgcua ggcaugaaca aaugcuacau acaaaucaug      180

gaccucggac acaugugcga cgcaacaaug agcuacgaau gccccaugcu cgacgaggga      240

gucgaacccg acgaugucga cugcuggugc aacacaacca gcaccugggu cguguacgga      300

accugccacc acaaaaaggg cgaagcgcgc cgcucccggc gcgccgucac ccuccccagc      360

cacucaacac gaaaacuaca aacacgaagc caaacauggc uggaaucacg agaauacacc      420

aaacaccuaa uacgagucga aaacuggaua uuccgaaacc ccggcuucgc acuagccgcc      480

gcggccauag ccuggcuccu cggcucauca accucccaaa aaguaaucua ccucguaaug      540

auacuccuaa uagcccccgc auacucgaua agaugcauag gcgucagcaa ccgcgacuuc      600

guagaaggaa ugagcggcgg caccuggguc gacguugucc uagaacacgg cggaugcguc      660

acaguaaugg cccaagacaa accuaccguc gacauagaac ucgucacaac aaccguuucc      720

aacauggccg aaguacgauc auacugcuac gaggcuucaa uauccgacau ggccucggac      780

ucccgcugcc ccacccaagg cgaagccuac cucgacaaac agagcgacac ccaauacguc      840

ugcaaacgca cgcuagucga ccgcggaugg ggcaacggcu gcggacuauu cgggaaagga      900

ucccuaguaa caugugcaaa auucgccugc ucaaagaaaa ugacggguaa aucaauccaa      960

ccggaaaacc ucgaguaucg aaucaugcuc uccguccaug gcucccaaca cuccggcaug     1020

auaguaaaug acacaggcca cgaaaccgau gaaaacagag cuaaaguuga aauaaccccc     1080

aacucaccac gcgccgaagc cacccugggc ggcuuuggau cacugggccu cgacugcgaa     1140

ccccgaaccg gucucgacuu cagcgaccug uacuaccuaa caaugaacaa caaacacugg     1200

cuaguccaca aggagugguu ccacgacauc ccacucccau ggcacgccgg agcagacaca     1260

ggcaccccac acuggaacaa caaggaagcc cuagucgagu ucaaagacgc acacgcaaaa     1320

cgacaaacgg ucgucguccu agggagccaa gaaggagcag uacacacagc acucgcaggg     1380

gcgcuagaag cagaaaugga cggggcaaaa ggacgccuuu ccagugggca cuuaaagugc     1440

agacucaaaa uggacaaacu uagauuaaaa ggcguaucau acucacuaug caccgccgcc     1500

uucaccuuca ccaaaauacc cgccgaaacg cugcacggaa cagucacggu ugaaguccaa     1560

uacgccggca cagacgggcc cugcaaagua cccgcgcaaa uggcggucga caugcagaca     1620

cuuacccccg uuggucggcu aauaacggcc aacccaguca uaacagaaag caccgaaaac     1680

uccaaaauga ugcuagaacu cgacccaccc uucggagaca gcuacaucgu caucggcguu     1740

ggcgaaaaga aaaucacaca ucacuggcac agaucaggua gcaccauagg caaagccuuu     1800

gaagccacag ucagaggcgc caggcguaug gcaguccucg gagacaccgc cugggacuuu     1860

ggauccgugg gaggcgcacu aaacucccua ggcaaaggca uccaucaaau auucggggcc     1920

gccuucaaau cgcuguucgg cggaauguca ugguucagcc aaauccuaau cggaacgcuc     1980

cuuauguggc ucggccuaaa caccaaaaac ggauccauau cccucaugug ccuggcccuc     2040

ggaggcgucc uaaucuuccu guccacagcg gucuccgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9593
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX806557.2 prME-short mRNA Design 3 opt

<400>  9593
gggagaaagc uuaccaugac cuccguuggc aucgugggcc uccuccuaac aaccgcaaug       60

gccgccgaag uaacccgacg uggaagugca uacuacaugu accuagaccg caacgacgca      120

ggagaagcaa ucucauuccc aaccacccua ggcaugaaca aaugcuacau ccaaaucaug      180

gaccuaggcc acaugugcga cgcaaccaug ucauacgaau gccccaugcu agacgaaggu      240

guugaaccag acgacguaga cugcuggugc aacacaacau ccaccugggu agucuacggc      300

acaugccacc acaaaaaggg agaagcacgg cgcucccgcc gagccgucac ccugcccagu      360

cacuccaccc gcaaacucca aacccgaucc caaaccuggc uagaaagccg cgaauacaca      420

aaacaccuaa uaagaguaga aaacuggaug uucagaaacc ccggauucgc ccucgccgca      480

gcagccauag cauggcuccu cggcucaucc accucacaaa aaguuauaua ccucgucaug      540

auccuacuaa uagccccagc cuauagcauc agaugcaucg gcgucuccaa ccgagacuuc      600

gucgaaggaa ugucaggagg aaccugggug gacgucgugc ucgaacacgg gggcugcgug      660

accguaaugg cacaagacaa accaacggua gacaucgagc uagucacaac aacugugucc      720

aacauggccg aaguacgaag cuauugcuac gaagcaucca ucucggacau ggccagcgac      780

ucucgaugcc ccacccaagg cgaagcauac cuggacaaac aauccgacac ccaauacgua      840

ugcaaacgua cucuaguaga ccgcggcugg gggaacgguu gcggacuguu cggaaaaggg      900

ucacugguaa ccugcgcaaa guucgcgugc ucuaagaaaa ugaccggaaa auccauccaa      960

ccagaaaacc uagaauaccg caucaugcua uccguccacg gaucccagca caguggaaug     1020

auugucaacg acaccggaca ugaaacagac gaaaaccgcg ccaaaguaga aauaacacca     1080

aacucacccc gcgccgaagc aacacucggc ggauucggcu cacucggacu cgacugcgaa     1140

ccccgcaccg gccucgacuu cucagaccug uacuaccuca caaugaacaa caaacacugg     1200

cucguacaca aagaaugguu ccacgacaua ccacucccuu ggcacgcggg cgccgacaca     1260

gguaccccac acuggaacaa caaagaagcc cuagucgagu ucaaagacgc acacgccaaa     1320

agacagacgg uagucgugcu cggaagccag gaaggagccg uacacaccgc ccucgccggg     1380

gcacuggaag cagaaaugga cggcgcuaaa ggccgccucu cauccggcca ccuaaaaugc     1440

cgguuaaaaa uggacaaacu gagacucaaa ggaguaagcu acuccuuaug caccgccgcc     1500

uucaccuuca ccaaaauacc ggccgaaacc cuccacggua ccguaacagu agaaguccaa     1560

uacgccggaa cagacggccc cugcaaaguc ccugcccaga uggcugucga caugcaaacc     1620

cucacccccg ucggaagacu aaucacugcc aaccccguca uaaccgaauc caccgaaaac     1680

agcaaaauga uguuggaacu cgauccccca uuuggcgacu cauacaucgu gauaggcgua     1740

ggcgagaaaa agauaacaca ccacuggcac cguuccggaa guaccaucgg caaagcauuc     1800

gaagcgaccg uccgaggcgc aaaacgaaug gccguacucg gggacacggc cugggacuuc     1860

gggagcgugg gcggcgcacu aaacucccuc ggcaaaggaa uccaccaaau cuucggcgcc     1920

gccuucaagu cccucuucgg aggcaugucg ugguucuccc agaucuuaau cggaacucua     1980

cucauguggc uaggguugaa caccaaaaac ggaucaauau cccuaaugug cuuagcccuu     2040

ggaggaguac uaaucuuccu aagcaccgca gugucagccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9594
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC191864.1 prME-short mRNA Design 3 opt

<400>  9594
gggagaaagc uuaccaugac caguguaggc auagugggac uacuacucac uaccgccaug       60

gcagccgaag uaacaaggcg aggauccgcg uacuacaugu acuuagaccg caacgacgca      120

ggagaagcaa ucuccuuucc caccacacuc ggaaugaaca aaugcuacau ccaaauaaug      180

gaccuaggcc acaugugcga cgccacaaug uccuacgaau gcccgaugcu cgacgaagga      240

gucgagccag augacguaga cugcuggugc aacaugacca gcaccugggu cgucuacggc      300

accugccacc acaaaaaagg agaggcgcga agauccagac gggccgucac acuaccaagu      360

cacuccacgc guaaacucca gaccagaagc caaaccuggc ucgaaucgcg agaauacaca      420

aaacaccuaa uccgcgucga gaacuggaua uuccguaacc ccggauucgc ccuagcagcc      480

gccgcaaucg ccuggcuacu aggcagcuca accagccaaa aaguuaucua ccuugucaug      540

auacuccuca uugcgccagc auacucgauc agauguauug gugucaguaa ccgcgacuuc      600

guagaaggca uguccggcgg aacauggguc gacgucguuc ucgaacacgg gggaugcguc      660

accgucaugg cacaagacaa accaaccguc gacauagaac ucguaacuac aaccgucucc      720

aacauggccg aaguccgcag cuacugcuac gaagccagca ucucagacau ggcgagcgau      780

ucccgcugcc ccacgcaagg cgaagccuac cuagacaaac aaagcgacac ccaauacgua      840

uguaaacgga cccucgucga cagaggaugg ggcaacggcu gcggccuuuu cggcaaaggc      900

agccuaguaa ccugcgccaa auucgccugc agcaaaaaaa ugaccggaaa aagcauucaa      960

ccugaaaacc uagaguaccg caucaugcuc uccguccacg gaucgcaaca cuccggcaug     1020

auagucaaug acaccggaca cgaaaccgac gaaaaccgug cgaaagugga aaucaccccu     1080

aauagcccca gggccgaagc cacacucggc ggauucggca gccuagggcu agacugcgaa     1140

cccagaacag gccuagacuu cagcgaccua uacuaccuca caaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccacuaccau ggcacgcugg agccgacaca     1260

gggacucccc acuggaacaa caaagaagcg cucguagagu ucaaagaugc acacgcaaaa     1320

cggcaaacag ucguaguccu aggcucacag gaaggcgcgg uccauacagc ccucgcagga     1380

gcacucgaag cagaaaugga cggagccaaa ggccgacuaa gcagcgggca ccucaaaugc     1440

agacucaaaa uggacaaacu cagacugaaa ggcguaagcu acagccuuug cacagcagcc     1500

uucaccuuua ccaaaauacc cgccgaaacc cuacacggca ccguaacggu agaaguucaa     1560

uacgcaggca ccgacggacc augcaagguc cccgcacaaa uggccgucga uaugcagacc     1620

cuaacccccg ucggacgccu caucaccgcc aacccaguca uaaccgaaag cacagaaaac     1680

ucgaaaauga ugcucgaacu agacccaccc uucggagacu cauacaucgu aaucggaguc     1740

ggagaaaaaa aaauaacaca ucacuggcac cgauccggcu caacaauagg caaagccuuc     1800

gaagcaacag uacgaggagc gaaacggaug gccguacucg gagacacagc cugggacuuc     1860

ggcagcgucg gaggagcccu caacagcuua ggaaaaggca uccaccaaau auucggagcc     1920

gcauucaagu cccuguucgg cggcaugucc ugguucagcc aaauacuaau aggcacgcua     1980

cugauguggc ucggacucaa caccaaaaac ggcagcauca gccucaugug ccucgcgcuc     2040

ggaggcgucc ucauauuccu uucaaccgca gucucagcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9595
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 prME-short mRNA Design 3 opt

<400>  9595
gggagaaagc uuaccaugac aagcgucggg aucgugggcc uccuacuaac gaccgcaaug       60

gcagcagagg ucacccgcag aggaagcgca uacuacaugu accuagaccg aaacgacgcc      120

ggagaagcaa ucagcuuccc cacaacccuc ggcaugaaca aaugcuacau acagaucaug      180

gaccuaggac acaugugcga cgccaccaug agcuacgaau gcccgaugcu agacgaaggc      240

gucgaaccag acgacgucga cugcuggugc aacacaacaa gcacaugggu agucuacgga      300

accugccacc auaagaaagg cgaagcgcgc agaagccggc gagcaguaac ccuaccaagc      360

cacuccacaa gaaaacuaca aaccagauca cagaccuggc ucgaaagccg ggaauacacc      420

aaacaccuca uccgcgucga aaacuggauc uucagaaauc ccggcuucgc ccuagcggca      480

gccgccaucg ccuggcuauu aggcagcagu accucccaga aaguaauaua cuuagucaug      540

auacuacuaa uagcgccagc guacucgaua cgaugcauag gagucagcaa ccgagacuuc      600

gucgaaggca ugagcggagg gacgugggug gacguaguac ucgaacacgg aggcugcguc      660

acggcgaugg cucaagacaa acccacaguc gacaucgagc uagucaccac aaccguaagc      720

aacauggccg agguaagauc auacugcuac gaggcaucca uaagcgacau ggcaagugac      780

ucaagaugcc ccacacaagg agaagcauac cucgacaaac aguccgacac ccaauacguc      840

uguaaaagaa cccuaguaga cagaggcugg ggcaacgggu gcggacuauu cggcaaaggc      900

agccucguga caugcgcgaa auucgcuugc uccaagaaaa ugacaggcaa auccauccaa      960

cccgaaaacc ucgaauaccg cauaaugcua agcguacacg ggucccaaca cuccggcaug     1020

cuggugaacg auaccggaca cgagaccgac gaaaacagag cgaaaguaga aauaacccca     1080

aauagcccgc gagcagaagc aacucuaggc ggauucggca gccuuggacu agacugcgaa     1140

ccaagaacag gccucgacuu cucagaccua uacuaccuca ccaugaacaa caaacacugg     1200

cucgcgcaca aagaaugguu ccacgauauc ccccuccccu ggcacgcagg cgcagcaacg     1260

ggaacaccac acuggaacaa caaagaagcc cucgucgagu ucaaagacgc gcacgccaaa     1320

aggcaaaccg ucguaguacu aggaucccaa gaaggagccg uccacaccgc acucgccgga     1380

gcacuagaag ccgagaugga cggcgcaaaa ggacgacucu ccagcggcca ccucaaaugc     1440

cgacucaaaa uggacaaacu gcgccuuaaa ggcgucucgu acuccuuaug cacggccgcc     1500

uucaccuuua caaaaauacc cgcagagacc guagacggca ccgucaccgu agaagggcag     1560

uacgggggaa ccgauggacc uugcaaagua ccagcccaaa uggcaguaga caugcagacc     1620

cuuacacccg uaggacgacu aauaacagcc aaccccguca uaaccgaauc caccgaaaac     1680

agcaaaauga ugcuagaacu agauccccca uucggcgacu cauacaucgu gaucgggguc     1740

ggagaaaaga aaauaaccca ccacuggcac aggagcggga gcacaaucgg caaagccuuc     1800

gaagccaccg uacggggcgc caaacgaaug gccguucucg gcgacacagc augggacuuc     1860

ggcucggucg gcggagcacu aaacagccuc ggaaaaggaa uacaccaaau aauaggcgcg     1920

gccuucaaau cacuauucgg gggcaugagc ugguucagcc aaauacugau agggacgcug     1980

cugauguggc ucggacugaa uacgaaaaac ggcuccauca gucuaaugug ccuagcccua     2040

ggaggcguac ugauauuccu cagcacagca guauccggcu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  9596
<211>  2281
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 prME-short mRNA Design 3 opt

<400>  9596
gggagaaagc uuaccauggg cgccgacgcc agcaucggca ucgucucccu acuccuaaca       60

accguaaugg cagcagagau aacgcgacga ggauccgcgu acuacaugua ccuagacaga      120

agcgacgccg gcaaggccau cucauucguc acaacgcuag gcgucaacaa augccacguu      180

caaauaaugg aucucggcca caugugugac gcuaccauga gcuacgaaug cccaaugcuc      240

gacgagggcg ucgaacccga cgacguggac ugcuggugca acaccaccuc aacauggguc      300

guauauggca cgugccacca caagaaaggc gaagcccgcc gaucaaggag agccgucacc      360

cucccaagcc acucaaccag aaaacuccaa accagaagcc agaccuggcu cgagagccga      420

gaauacacaa aacaccugau aaaaguagaa aacuggaucu uccgaaaccc aggcuucgcg      480

cuagucgcag uagccauagc cuggcuccuc ggcagcucca caucacagaa gguuauauac      540

cuagucauga uacuccucau cgccccagca uacagcaucc gcugcauagg cguaagcaac      600

cgcgacuucg ucgaggggau guccggagga acgugggucg acguagugcu agaacacggu      660

ggcugcguaa ccgucauggc gcaagacaaa ccgaccgucg acaucgagcu agucacaacg      720

acaguaucaa acauggccga aguacgaagc uacugcuacg aagccagcau cagcgacaug      780

gccucagaca gcagaugucc aacccaagga gaagcauacc ucgacaaaca aagcgacacg      840

caauacgucu gcaaacgcac ccuaguagac cguggauggg gcaacggcug cggccuguuu      900

ggaaaaggcu cacuggucac augugccaaa uuuacaugca gcaagaaaau gacgggcaaa      960

agcauacaac cagaaaaccu agaauaccgc aucaugcuaa gcguccacgg aagccaacac     1020

uccggaauga uagucaacga cgaaaaccga gcaaaagucg aaguaacacc caacucacca     1080

agagcagaag caacacucgg cggcuucgga agccugggcc uagacugcga acccagaacc     1140

ggacuagacu ucagcgaccu guacuaccuc accaugaaca acaaacacug gcucguccac     1200

aaggaauggu uccacgacau accacuacca uggcacucag gagccgacac cgaaacccca     1260

cacuggaaca acaaagaagc acugguggaa uuuaaagacg cacacgccaa aagacagacc     1320

gucguagucc ucgggagcca agaaggcgcc guccauacag cacuggccgg agcccuagag     1380

gccgaaaugg acggagcaaa aggacgccua ucauccggcc accucaaaug ccgccucaaa     1440

auggacaaac uccgacuaaa aggcguaucc uacucccugu gcaccgccgc auucacauuc     1500

acaaaaguac cagccgaaac acugcacggc acggugaccg uagaagucca auacgcagga     1560

cgcgacggcc caugcaaggu ccccgcacaa auggcaguag acaugcagac auuaacgcca     1620

gucggaagac ucauaaccgc caaccccguc auaacugaaa gcaccgaaaa cucaaaaaug     1680

augcuugaac uagacccacc cuucggagac uccuauaucg ucauaggagu aggcgacaag     1740

aaaauaacac accacuggca cagaucaggc agcauaaucg ggaaagccuu cgaggccaca     1800

guccgcggcg ccaaacgcau ggcaguccua ggggacacag caugggacuu cggcuccgug     1860

ggcggaguuu ucaacucccu aggaaaggga auccaccaaa ucuucggcgc cgccuucaaa     1920

ucacucuucg gaggaauguc cugguucucu caaauccuaa ucggaacccu acucgucugg     1980

cucggccuaa acaccaagaa cggcagcaua agccuaaccu gccucgcgcu cggaggcgua     2040

augauauucc ucucaacugc ggucagcgca ugaggacuag uuauaagacu gacuagcccg     2100

augggccucc caacgggccc uccuccccuc cuugcaccga gauuaauaga ucuaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaugc     2220

aucccccccc cccccccccc cccccccccc cccaaaggcu cuuuucagag ccaccagaau     2280

u                                                                     2281


<210>  9597
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 prME-short mRNA Design 3 opt

<400>  9597
gggagaaagc uuaccauggg agcagacacc agcaucggaa ucauaggacu acuccuaaca       60

acagcuaugg cggccgaaau cacgagaaga ggaagcgcau acuauaugua ccucgaccgu      120

uccgacgcag gcaaagcgau aucauucgca accacaaugg gcgugaacaa augccacguc      180

caaaucaugg accuagguca caugugugac gccaccaugu cuuacgaaug ccccaugcuc      240

gacgaaggag ucgaacccga cgacguagac ugcuggugca acaccaccuc cacauggguu      300

guguacggaa caugccauca caagaaagga gaagcccggc cgucccgccg cgcaguaacg      360

cucccgagcc acuccaccag aaaacuacaa acccgcucac aaacauggcu cgaaagucgc      420

gaguacacaa aacaucucau aaaagucgaa aacuggauac uacgcaaccc aggcuucaca      480

guggccgcgc ucgccauggc cuggcuacug ggaaacagua cgagccagcg cguaaucuac      540

cucguaauga uucuccugau cgcacccgca uacuccauac gcugcaucgg cguuagcaac      600

cgcgacuucg uagaaggaau guccggagga accuggguag acgugguccu agaacacgga      660

ggaugcguca ccgucauggc ccaagacaaa ccaacagucg acauagagcu aguaacaacc      720

acggucucca acauggccga agucagaagc uacugcuacg aagcauccau cagcgacaug      780

gcaagcgacu cccgcugucc cacacaaggg gaagccuacc ucgacaaaca guccgacaca      840

caauacgucu gcaaacgaac ccucguggau cgcggauggg gcaacggaug cggacucuuc      900

ggaaaaggaa gccuagugac cugugcaaag uucacgugcu ccaagaaaau gacaggaaaa      960

uccauacaac ccgaaaaccu cgaauaccgg aucaugcuca gcguccacgg aucccagcac     1020

agcggaauga uagugaacga cauaggccac gaaaccgacg aaaaccgagc aaaaguggaa     1080

gucaccccua auucaccccg cgcagaagca acacugggcg gcuucggcag ccucggccua     1140

gacugcgaac cgcgcaccgg acuagacuuu ucagaccugu acuaucuaac aaugaacaac     1200

aaacacuggc uuguccauaa agaaugguuc cacgacaucc cccucccaug gcaugcaggc     1260

gcagacacag gcacacccca cuggaacaac aaagaggcac ucguggaguu caaagacgca     1320

cacgccaaaa gacaaaccgu cguaguacuc ggcucccaag aaggagcagu ccacaccgcu     1380

cuggcaggag cauuggaggc agaaauggac ggcgcgaagg gccgacucuu cucaggacac     1440

cucaaaugcc ggauaaaaau ggacaaacua aggcuaaaag gcgucagcua cucacucugc     1500

accgccgcau ucacauucag caaagucccg gcagaaacgc uccacggaac agucacggug     1560

gaggugcaau acgccggccg agacggaccc ugcaaagucc ccgcccaaau ggcaguagac     1620

augcaaacgc ucacccccgu aggaagacuu aucacggcca acccaauaau aacagaaucc     1680

accgaaaaca gcagaaugau gcucgagcuc gacccucccu ucggcgacuc auacauaguu     1740

aucggaguag gcgacaagaa aaucacgcac cacuggcaca ggucaggcag cacaaucgga     1800

aaagccuucg aagccaccgu ccgcggcgca aaaagaaugg ccguacucgg cgacacagcc     1860

ugggacuuug gaagcgucgg cggagucuuc aacucacuag ggaaaggagu ucaccaagua     1920

uucggagccg ccuucaaauc gcuauucggc gggauguccu gguucucgca aauccucauc     1980

ggaacccuuc uagucuggcu cggacugaac accaaaaacg gaagcaucuc ccuagcaugc     2040

cuagcccucg gcggugcaau gauauuccua agcacggccg uaagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  9598
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU321639.1 solE mRNA Design 3 opt

<400>  9598
gggagaaagc uuaccauguc gcaaaagguc auauaccugg ucaugaucuu acucauagcc       60

ccggcauaca gcauaagaug uauaggaguc ucgaacagag acuucgucga aggaaugagc      120

ggaggcacgu ggguggacau aguccuggaa cacggaggau gcgugaccgu gauggcgcaa      180

gacaaaccga ccguggacau agaacuggua acaaccacgg ucucgaacau ggccgagguc      240

aggagcuacu gcuacgaagc aagcauaagc gacauggcaa gcgauaguag augcccgaca      300

caaggcgagg cauaucuaga caaacaaagc gauacccaau acguauguaa gcgcacauua      360

guggaccgag gauggggaaa cggcugcgga cuguucggga aaggaagccu agucacgugu      420

gcgaaguucg cgugcagcaa gaaaaugacc ggcaaaucga uacaacccga gaaccuagaa      480

uacagaauaa ugcuaagcgu acacgggagc cagcacucgg gcaugaucgu aaacgacacc      540

ggacacgaga ccgacgaaaa ccgagcgaaa guggaaauaa cacccaacuc gccccgagca      600

gaggccaccc uaggaggauu cggcucgcuc gggcuagacu gcgagcccag gacuggucua      660

gacuucucag acuuguacua ccucacgaug aacaacaagc acuggcuagu acacaaggaa      720

ugguuccacg acauaccacu gcccuggcac gcgggagccg acacagguac accccacugg      780

aauaacaagg aggcccucgu ggaguucaaa gacgcccacg caaaacgcca aaccguggua      840

guacucggca gccaggaagg ggccguccac acggcccugg caggggcacu ggaagcggaa      900

auggacgggg caaagggcag acuaucgagc ggacaccuaa aaugucgccu aaaaauggac      960

aagcuaaggc ucaagggcgu aaguuacagc cuaugcacgg ccgcauucac guucacgaaa     1020

auacccgcag aaacgcuaca cggcacgguc acggucgagg uccaauacgc gggcacagac     1080

ggacccugca aagugcccgc gcaaauggcc guggacaugc aaacgcucac gcccgucgga     1140

cggcucauaa ccgcaaaccc gguaauaacc gaaaguacgg aaaacagcaa aaugaugcuc     1200

gaguuagacc caccauucgg ggacucauac auaguaauag gcgugggcga gaaaaagaua     1260

acgcaccacu ggcaccgcuc gggaucuacc aucggcaagg cuuuugaagc aacaguacgc     1320

ggggcaaaac gcauggccgu gcucggggac acggccuggg acuuuggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9599
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312312.1 solE mRNA Design 3 opt

<400>  9599
gggagaaagc uuaccauguc gcaaaaaguc aucuaccuag ucaugauccu acucauagcc       60

cccgccuaca gcauccgcug caucggaguc ucaaaccgcg acuucguuga aggcauguca      120

ggaggcacau gggucgacgu gguacuagaa cacggcgggu gcgucacagu cauggcccaa      180

gacaaaccca caguggacau cgaacugguc accaccacag uaucaaacau ggcagaaguu      240

agaagcuacu gcuacgaggc aagcauaucc gacauggcca gcgacucaag augcccaaca      300

caaggcgaag ccuaccugga caaacaaagc gacacucaau acguaugcaa acgaacgcua      360

guggacagag gauggggaaa cggauguggc cucuucggca aaggcagccu cgucacaugc      420

gcaaaauucg ccugcucaaa gaaaaugacc ggaaaaucca uucaacccga gaaccuagaa      480

uacagaauca ugcuauccgu gcacggcagc caacacuccg gaaugauagu caacgacacc      540

ggccacgaaa ccgacgaaaa ccgcgcaaaa guggaaauaa ccccaaacuc acccagagcc      600

gaagcaacac uaggcggcuu cggcucccuc ggacuagacu gcgaacccag aaccggccuc      660

gacuucucag accuauacua ucuaaccaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauaccccu acccuggcac gcaggggccg auacaggcac accccacugg      780

aauaacaaag aagcccucgu agaguucaaa gaugcacaug caaaacgaca gaccguaguc      840

gugcucggca gucaggaagg cgccguccac acggcccugg ccggagcccu cgaagcagaa      900

auggacggcg caaaaggccg acucuccagc ggacaccuaa aaugccgccu aaaaauggac      960

aaacuacgcc ucaaaggcgu cagcuacagc cucugcacag cagcauucac guucacaaaa     1020

auaccagcag aaacacucca cggcacagua acagucgaag uccaguacgc cggcacagac     1080

ggacccugca aaguaccagc acaaauggca guagacaugc aaacgcucac gcccguagga     1140

cgccuaauca ccgcaaaucc agucaucacc gaaagcaccg aaaacagcaa aaugaugcuc     1200

gaacucgauc ccccauucgg cgacucguac auagucaucg gcgugggaga aaagaaaauc     1260

acacaccacu ggcaccgcuc cggaagcacc auaggcaaag ccuucgaagc caccguacgc     1320

ggagccaaac gcauggcagu acucggagac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9600
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AY632535.2 solE mRNA Design 3 opt

<400>  9600
gggagaaagc uuaccauguc gcaaaaagua auauaccuag ucaugauccu acucaucgcc       60

ccagcuuaca gcauacgcug caucgggguc ucaaacaggg acuucgucga aggcaugagc      120

ggaggaacau ggguggacgu gguacuagaa cacggcggau gcguaaccgu aauggcgcaa      180

gacaagccua ccguagacau cgaacuagua accaccacag ucuccaacau ggccgaaguc      240

aggaguuacu gcuacgaagc aagcauaagc gacauggcga gcgacagccg gugcccgaca      300

caaggugaag cauaccuaga caaacaaagu gacacccaau acgucugcaa aaggacccua      360

guagacaggg gcuggggcaa cggcugcggu cuauucggaa aaggcagccu agucacaugc      420

gccaaauuca ccugcagcaa aaaaaugacc ggcaaaucaa uccaacccga aaaccucgaa      480

uaccgcauaa ugcugagcgu gcacggguca caacacucgg gaaugaucgg auacgaaaca      540

gacgaagacc gcgcaaaagu agaaguaaca ccgaacucgc caagagcaga agccacccuc      600

ggaggauucg gcucucucgg acucgacugu gaaccaagaa ccgggcuaga cuucucagac      660

cucuacuacc uaacgaugaa caacaaacac uggcucgugc acaaagaaug guuccacgac      720

auaccccugc ccuggcacgc aggcgcagac acgggcaccc cacacuggaa caacaaggaa      780

gcacuaguag aauucaaaga cgcccacgcc aagcgucaaa caguagucgu gcucgggagc      840

caagaagggg ccguacacac ggcccuagca ggggcccuag aagcagaaau ggacggggca      900

aaaggaagac uauucuccgg acaccugaaa ugccgccuaa aaauggacaa acucagacua      960

aagggcguaa gcuacagccu augcacagcc gcauucacau ucacaaaagu ccccgcagaa     1020

acacuacacg gcaccgucac cgucgaaguc caauacgcgg gcacagacgg cccauguaaa     1080

auaccaguac aaauggcagu agacaugcaa acacucacgc cggugggcag acucauaaca     1140

gcaaacccag ucauaaccga aagcacagaa aacaguaaaa ugaugcuuga acucgaccca     1200

cccuucggug acucguacau cgucauagga guaggcgaca aaaaaaucac gcaccacugg     1260

caccgaucag ggucaaccau uggcaaagca uucgaagcaa caguacgcgg ggccaaacgc     1320

auggccgucc uaggggacac cgccugggac uucggaucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  9601
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU527068.1 solE mRNA Design 3 opt

<400>  9601
gggagaaagc uuaccauguc ccaaaaagua auauaccuag uaaugauccu ccucaucgca       60

ccggccuaua guauccguug cauaggaguc agcaacagag acuuuguaga aggcaugagc      120

ggcggcaccu gggucgacgu cguccuggaa cacggaggau gcgugaccgu aauggcacaa      180

gacaaaccga ccguggacau cgaacugguc acaacaacag ucucaaacau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcauaagc gacauggcga gcgacagccg augcccaacc      300

cagggagaag cauaccucga caaacagagc gacacacaau acguaugcaa acgaacgcuc      360

guagacaggg gcuggggcaa cggaugcggc cuauucggaa aagggagccu agucacgugc      420

gcgaaauucg ccugcagcaa gaaaaugaca ggaaaaucaa uccagccaga aaaccuagag      480

uaccgaauca ugcugagcgu acacgggagc caacacucag gcaugaucgu aaacgacacc      540

ggacacgaaa ccgacgaaaa ccgcgcaaaa guagaaauaa ccccaaacuc accaagggcc      600

gaagcaacgc uaggcggauu cggcucacuc ggacucgacu gcgagccaag aaccggacuc      660

gacuucagug accuguacua ccucacaaug aacaacaaac acuggcuagu acacaaagaa      720

ugguuccacg acauaccacu ccccuggcac gcgggcgcag acacgggcac accacacugg      780

aacaacaaag aagcccucgu agaguucaaa gacgcacacg ccaaacgaca gaccguggua      840

guacucggaa gccaggaagg ggccgugcac accgcccugg ccggcgcccu ggaagccgaa      900

auggacggag caaaaggccg ccucucaagc gggcacuuaa aaugucgccu aaaaauggac      960

aaacucaggu uaaaaggcgu aagcuacagu cuaugcacgg ccgcauucac auucaccaaa     1020

auaccagcgg aaacgcuaca cggcacaguc acggucgaag uccaauacgc gggcacagac     1080

gggcccugua aaguaccagc acaaauggcc guagacaugc aaacacuaac accggucggc     1140

cgacucauaa ccgcgaaccc agucauaacc gaaagcacag aaaacaguaa gaugaugcua     1200

gaacuagauc ccccauucgg agacagcuac auagucauag gcguagguga gaaaaagaua     1260

acacaccacu ggcacagaag cggaucaacc auaggaaagg ccuucgaagc aaccgugaga     1320

ggggcaaaaa gaauggccgu acucggcgac accgccuggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9602
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX421193.1 solE mRNA Design 3 opt

<400>  9602
gggagaaagc uuaccauguc gcaaaaagua aucuaccuag ucaugaucuu gcuaauagcc       60

ccggccuaca gcauacgcug caucggagua ucaaacaggg acuucgucga aggaaugagc      120

ggaggcacgu ggguagacgu gguacuagaa cacggcggau gcgucaccgu aauggcgcaa      180

gacaaaccaa ccguggacau cgaacucgua acgaccacgg uaucaaacau ggccgaagua      240

agaagcuacu gcuacgaagc aagcaucagc gacauggcaa gcgauagccg gugcccgacc      300

caaggagaag cauaccuaga caaacagagc gacacccaau acguaugcaa acgaacccua      360

guagacaggg gcuggggcaa cggcugcgga cuauucggaa aagggagccu agucacaugc      420

gccaaauuca caugcagcaa gaaaaugacc gggaaaucaa uccagcccga aaaccuagaa      480

uaccgcauaa ugcuaagcgu gcacgggagu cagcacucug gcaugaucgu gaacgacacg      540

ggauacgaaa cagacgaaaa cagagcaaaa guagaaguaa caccaaacuc cccaagagcc      600

gaagccacgc ucgggggauu cggaucgcuc ggacuagacu gcgaaccccg uaccgggcua      660

gacuucucag accuauacua ccuaacaaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acauaccacu gcccuggcac gcgggcgccg acacuggaac accacacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacaug caaagagaca gaccgugguc      840

guacugggaa gccaagaagg agcaguacac acagcacuag caggggcacu agaagcggaa      900

auggacgggg ccaaaggaaa acuauucagu ggacacuuaa agugcagacu aaaaauggac      960

aaacuaagac ucaagggcgu aagcuacagc cuaugcacag cagcguucac guucacgaaa     1020

gugccagcgg aaacgcuaca cggaaccguc acugucgaag uccaauacgc gggcacagac     1080

gggcccugca aaauccccgu ccaaauggcc guagacaugc aaacgcuaac acccguggga     1140

agacuaauaa ccgcaaaccc agucauaaca gaaagcacgg aaaauagcaa aaugaugcuc     1200

gaacuagacc caccguucgg agacagcuac auaguaauag gcguaggaga caagaaaauc     1260

acccaccacu ggcaccgcuc gggcucaacc auaggaaaag ccuucgaagc gaccguacgc     1320

ggagccaaac gaauggccgu acuaggagac acggcauggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9603
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LC002520.1 solE mRNA Design 3 opt

<400>  9603
gggagaaagc uuaccauguc acagaaagua auauaccuag uaaugauacu ccucauagcc       60

ccggccuacu ccauacgcug caucggaguc agcaacagag acuucguaga gggaaugagc      120

ggcggaacau ggguagacgu gguccuagaa cacggcggau gcguaacagu aauggcacaa      180

gacaaaccaa ccguggacau agaauugguc acaaccacag uaucgaacau ggccgaaguc      240

agaaguuacu gcuacgaagc aagcauaagc gacauggcaa gcgacagccg cugccccacc      300

caaggagagg cauaccucga caaacaaagc gacacacaau acguaugcaa acgaacacuc      360

guagaccggg gcugggguaa cggcugcggc cuauucggga aagggagccu agucacaugc      420

gcgaaguuca caugcagcaa gaaaaugacc ggcaaaagca uccagcccga gaaccuagaa      480

uacagaauaa ugcucagcgu gcacgggagc caacacucgg gcaugaccgu aaacgacauc      540

ggcuacgaaa cagacgaaaa ccgcgccaaa guagaaguaa ccccgaacuc gccaagagcc      600

gaagccaccc uagguggauu cggaucccuc ggacucgacu gcgaaccaag aaccggacuc      660

gacuucucgg accuauacua ccucacgaug aacaacaaac acuggcuagu acacaaagaa      720

ugguuccacg acauaccccu acccuggcac gccggagcag acacaggcac gccacacugg      780

aacaacaaag aagcgcuagu agaguucaaa gacgcccacg ccaaacgaca aacaguggua      840

guguuaggaa gccaagaagg ggcaguccac acagcccugg caggagcacu cgaagcagaa      900

auggacggag ccaaaggcaa acucuuuagc ggacaccuca aaugccgccu aaaaauggac      960

aaacuccgcc uaaagggcgu aaguuacagc cugugcacgg cagcguucac cuucacaaaa     1020

gugcccgcgg aaacacucca cggcaccguc acggucgaag uccaauacgc aggaacggac     1080

ggacccugca agauacccgu acaaauggca guagauaugc agacucucac ccccgucgga     1140

agacucauaa ccgccaaccc gguaauaaca gaaagcacag aaaacagcaa aaugaugcuc     1200

gaacucgacc caccauucgg ggacucauac auagugaucg gcgugggcga caagaaaauc     1260

acccaccacu ggcaucgcuc gggcagcacc auaggcaaag ccuucgaagc aacaguccgc     1320

ggggcaaaac gaauggccgu auuaggggac accgcauggg acuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9604
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  DQ859059.1 solE mRNA Design 3 opt

<400>  9604
gggagaaagc uuaccauguc ccaaaagguc auauaccuug uaaugauccu acucauagcu       60

ccagcauaca gcauacgcug cauaggcgua ucaaacagag acuucguaga aggcaugagc      120

ggaggaacau gggucgacgu aguacucgaa cacggaggcu gcgucacagu aauggcacag      180

gacaaaccga ccguggacau agaacucguc acaaccacag ucuccaacau ggccgaagua      240

agaagcuacu gcuacgaagc aagcaucagc gacauggcgu cagacagccg augcccaaca      300

caaggcgaag ccuaccugga caaacagagu gacacccagu acgugugcaa acggacacuu      360

guagacagag gcuggggcaa cggcuguggc cuguucggaa aagggagccu agugaccugc      420

gcaaaauuca caugcagcaa gaaaaugaca ggcaaaucaa uccaaccaga aaaccucgaa      480

uacagaauaa uguuauccgu ccacggaagc caacacuccg gcaugauagu aaacgacgaa      540

aaccgcgcca aaguagaagu aacaccaaac ucacccagag ccgaagccac gcucggcggc      600

uucggaucgc uaggccuaga cugcgaaccc cgaacagggc ucgacuucag cgaccucuac      660

uaccucacga ugaacaacaa acacuggcua guacacaaag aaugguucca cgacaucccc      720

cuacccuggc acgcaggcgc agauacaggc acaccgcacu ggaacaacaa ggaagcgcuc      780

guggaguuca aagacgcaca cgccaagaga caaaccguag ucguacuagg aagucaggaa      840

ggcgccguac acaccgcccu cgccggagca cucgaagcag aaauggacgg agcaaaagga      900

agacucuuca gcggacaccu caaaugccgc cucaaaaugg acaagcuaag acucaaaggc      960

guaagcuaca gccuaugcac agccgccuuc acauucacaa aaguacccgc cgaaacacuc     1020

cacggcacag ucaccgucga aguccaauac gccggcacag acggacccug caaaguaccc     1080

gcacaaaugg ccgucgacau gcaaacgcuc acacccgucg gcaggcucau aaccgcaaac     1140

ccggucauca ccgaauccac ggaaaacagu aaaaugaugc ucgaacuaga ucccccauuc     1200

ggcgacucgu acauaguaau aggcgucggc gacaagaaaa uuacacacca cuggcaccga     1260

uccggaagca ccauaggcaa agccuucgaa gcaacagucc gcggcgccaa acgcauggcc     1320

guacuagggg acacggcaug ggacuuuggc ucgugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  9605
<211>  1570
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963573.2 solE mRNA Design 3 opt

<400>  9605
gggagaaagc uuaccauguc acaaaaaguc auauaccuag uaaugauacu gcuaauagca       60

ccagcauaua gcauacgcug caucggaguc uccaacagag acuucgucga gggcaugagc      120

gggggaacgu ggguggacgu aguacucgaa cacggcggau gcgucaccgu cauggcacaa      180

gacaaaccaa caguagacau agaacugguc acaaccaccg ucucaaauau ggccgaaguc      240

agauccuacu gcuacgaagc aagcaucucc gacauggcaa gcgacagccg augcccaacc      300

caaggcgaag ccuaccucga caaacaauca gacacgcaau acguuugcaa acgaacccuc      360

gucgaccgag gcuggggcaa cggcugcggc cucuuuggga aaggaagccu aguaacaugc      420

gcgaaauuca caugcuccaa gaaaaugacc ggcaaaucaa uccaaccaga gaaccuagaa      480

uaccgaauca ugcugagcgu acacggcagc caacacucag gaaugauagg guacgaaacc      540

gacgaaaauc gagccaaagu cgaagucacc ccaaacagcc cacgagccga agccacccuc      600

ggaggcuucg gaucacucgg acucgacugc gaaccccgga ccgggcucga cuucucagac      660

cuguacuacc ucacaaugaa caauaaacac uggcucguac auaaagaaug guuucacgac      720

auaccccucc ccuggcacgc cggcgcagac accggcacac cacacuggaa caacaaagaa      780

gcacucgugg aguucaaaga cgcccacgcc aaacgccaga ccgucgucgu acuaggcuca      840

caagaaggag caguccacac agcccuagca ggggccuuag aagcggaaau ggacggcgca      900

aaagggaaac ucuucagcgg acaccugaaa ugccgccuaa aaauggacaa acuacgacuc      960

aaaggcguaa gcuacagucu augcacggcc gcauucaccu ucaccaaagu cccagcagaa     1020

acacuccacg gcacagucac aguagagguu caauacgcag gcacagacgg ccccugcaaa     1080

auacccgucc aaauggccgu agacaugcaa acccuaaccc ccgucggcag acuaaucaca     1140

gccaacccgg ucaucacaga auccacagaa aacagcaaaa ugaugcucga acucgaccca     1200

ccauucggag acucuuacau agucauagga guaggcgaca agaaaaucac gcaccacugg     1260

caccgauccg gcuccacaau aggcaaagcc uucgaagcaa ccgugcgcgg cgcaaaaaga     1320

auggcagucc ucggagacac cgccugggac uucggcucau gaggacuagu uauaagacug     1380

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     1440

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1560

caccagaauu                                                            1570


<210>  9606
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075939.1 solE mRNA Design 3 opt

<400>  9606
gggagaaagc uuaccauguc gcagaaaguc auauaccugg ucaugauccu gcucauagcc       60

ccagccuaca gucuacgaug caucggagua agcaacagag acuucgucga aggaaugagu      120

ggaggcacau ggguggacgu cguccuggaa cacggcgggu gcgucacagu aauggcacaa      180

gacaaaccca ccguagacau cgagcuaguc acaaccacag ucuccaacau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcauauca gacauggcaa gcgacagcag augcccgacc      300

cagggggagg ccuaccucga caaacaaagc gacacccaau acgucugcaa acgcacccua      360

guggacaggg gcuggggcaa uggcugcggc cuguucggaa aaggcagccu ggucacaugc      420

gcaaaauucg ccugcucaaa gaaaaugaca ggaaaaagca uccaacccga aaaccuggaa      480

uaucgcauaa ugcuaucagu ccacggcagc cagcacucag gcaugauagu gaacgacacc      540

ggacacgaaa cagacgaaaa ccgcgcaaaa guagaaauaa ccccaaacuc accccgagcc      600

gaagcaacgc uuggcggcuu cggaucacuu ggacucgacu gcgaaccaag aaccggacua      660

gacuucagug accucuacua ccucaccaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauaccauu acccuggcac gcgggcgcag acacuggaac accccacugg      780

aacaacaaag aagcgcucgu ggaguucaaa gaugcccacg ccaaacgcca gaccgugguc      840

guacucggga gccaggaagg agcaguacac acggcccuag caggggcccu agaagcagaa      900

auggacggag caaaaggcag acucucaagc ggacacuuaa aaugccgccu gaaaauggac      960

aaacuacggc uaaaaggagu aaguuacagc cucugcacag ccgcauucac guucacgaag     1020

auaccggccg aaacacucca cggaacaguc acgguagaag uccaauacgc gggcacggac     1080

gggccaugca aaguacccgc gcaaauggca guagacaugc aaacgcuaac acccgugggc     1140

cgacucauaa cagcaaaccc cgucauaacc gaaagcaccg aaaacagcaa aaugaugcuc     1200

gaacucgauc ccccguucgg agacucauac auaguaauag gcguaggaga aaagaaaauc     1260

acgcaccacu ggcaccgcuc aggaucaacc auaggcaaag ccuucgaagc cacaguaaga     1320

ggggccaaac gcauggcagu gcuaggcgac acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9607
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447516.1 solE mRNA Design 3 opt

<400>  9607
gggagaaagc uuaccaugag caaaaaaguc auauaccucg ucaugauccu ccuuauagcc       60

cccgccuacu caauccgcug caucggugua agcaaccgcg acuucguuga aggaaugucc      120

ggcggaaccu gggucgacgu cguccucgaa cacggcggcu gcgugacugu cauggcacaa      180

gacaaaccca ccguagacau cgaacucguc acaaccaccg ucucgaacau ggccgaggua      240

cggagcuacu gcuacgaagc auccauauca gacauggccu ccgacuccag augcccaacu      300

caaggagaag ccuaccucga caaacaaucc gacacccaau acguaugcaa acgaacccua      360

guggaccgag gauggggcaa cggcugcgga cucuucggca aaggcucccu cgugacaugc      420

gccaaauucg ccugcucaaa aaaaaugacc ggaaaaucca uccaacccga aaaccucgaa      480

uacaggauca ugcuaagcgu ccacggauca caacacuccg gaaugaucgu caacgacaca      540

ggacacgaaa cagacgaaaa ccgagcaaaa guagaaauaa caccaaacuc ccccagagcc      600

gaagccaccc ucggcggcuu cggcucccua ggccuagacu gcgaaccaag aacgggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg auaucccccu ccccuggcac gccggcgccg acaccgggac cccccacugg      780

aacaacaaag aagccuuagu cgaauucaaa gacgcacacg caaaaagaca aaccgucguc      840

guccucggaa gccaagaagg cgcaguccac acagcccucg cgggagcccu agaagcagaa      900

auggacggag cgaaaggccg ccucuccagc ggccaccuca aaugccgccu caaaauggac      960

aaacuaagac ugaaaggcgu cuccuacucc cucuguaccg cagcauucac cuucacaaaa     1020

auccccgcag aaacccucca cggcaccguc accguagaag uccaauacgc aggcacggac     1080

ggccccugca aaguaccagc ccaaauggcc guagacaugc aaacccuaac ccccgucggc     1140

cgacucauaa ccgcaaaucc agucaucaca gaaucgaccg aaaacagcaa aaugaugcuc     1200

gaacuugacc ccccauucgg ggacuccuac auaguaaucg gcgucggcga aaaaaagaua     1260

acccaccacu ggcaccgcuc uggaucuacc aucggcaaag ccuucgaagc caccguacgc     1320

ggagcaaaac gcauggccgu ccucggcgac accgcauggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9608
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447515.1 solE mRNA Design 3 opt

<400>  9608
gggagaaagc uuaccauguc acaaaaagua aucuaccugg ucaugauccu acucauagcc       60

cccgcauaca gcauacgcug cauaggaguc ucaaaccgcg acuucgucga aggaaugagc      120

gggggaacgu ggguagacgu aguccuagaa cacggcggau gcguaaccgu aauggcgcaa      180

gacaaaccaa ccguagacau agagcuaguc acaacaacag ucucgaacau ggcagaaguc      240

aggagcuacu gcuacgaagc aaguauaagc gacauggcca gcgauucccg augcccgaca      300

caaggagaag cauaccuaga caaacaaagc gacacacaau acguaugcaa acgcacccua      360

guagaccgug gcuggggcaa cgguugcgga cuauucggga aaggaagucu ggucacaugc      420

gcaaaauucg caugcagcaa gaaaaugacc ggaaaaucga uacagcccga aaaccuagaa      480

uaccgaauaa ugcuaagcgu acacgggagc caacacucgg gaaugauagu aaacgacacc      540

ggacacgaga ccgacgaaaa cagagcaaaa guagaaauaa caccaaacuc gccucgagcc      600

gaagcaacac ucggaggcuu cggaucacuc ggacucgacu gcgagcccag aaccggccuc      660

gacuucucgg accuauacua ccucacaaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acauaccccu gcccuggcac gcaggcgccg acacgggaac gccccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacacg ccaaacgcca aaccgugguc      840

gugcucggga gccaagaagg agcagugcac acagcacugg ccggagcccu ggaagccgaa      900

auggacggag caaaaggaag gcucucaagc ggacaccuaa aaugccgccu gaaaauggac      960

aaguuaaggc uaaaaggagu aucauacagc cucugcacgg ccgcguucac cuucacaaaa     1020

auacccgcag aaacgcuaca cggcaccguc acagucgaag uacaguacgc gggaaccgac     1080

ggcccaugca aaguacccgc acaaauggcc guggacaugc aaacacuaac gccagugggc     1140

aggcuaauaa ccgcaaaccc agucauaacg gaaagcacgg aaaauagcaa aaugaugcuc     1200

gaacucgacc caccguucgg agacucguac auaguaauag gcguaggcga aaagaaaaua     1260

acacaccacu gguaccgcuc gggcucaacc auaggcaaag cauucgaagc aaccgugcga     1320

ggcgccaaac gcauggcugu acucggagac acagcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9609
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758874.1 solE mRNA Design 3 opt

<400>  9609
gggagaaagc uuaccaugag ccaaaaaguc auauaccuag ucaugauccu acuaaucgcc       60

ccagcauacu caauacgcug caucggcguc agcaaucgag acuucgucga aggcaugagc      120

ggcggcacau gggucgacgu aguccucgaa cacggcggcu gcgucaccgu cauggcccaa      180

gacaaaccaa ccguagacau agaacucguc acaacaacag ucucaaacau ggccgaaguc      240

agaagcuacu gcuacgaagc cuccauaagc gacauggcau ccgacucacg cugcccaaca      300

caaggcgaag cauaccucga caaacagucc gacacgcaau acguaugcaa acgcacccua      360

guagacaggg gcugggggaa cggaugcggc uuauucggca aaggcagccu cgucacaugc      420

gcgaaauucg ccugcuccaa gaaaaugacc ggcaaaucca uacagccaga gaaccuagaa      480

uaccgaauaa ugcucuccgu ccacggauca caacacagcg gaaugaucgu aaacgacacc      540

ggccacgaga ccgacgaaaa ccgcgccaaa gucgaaauca cccccaacag cccacgagcc      600

gaggccacac ucggaggcuu cggcucgcua ggacucgacu gugaaccuag aaccggccuc      660

gacuucucag accucuacua ccuaacgaug aacaacaaac acuggcuagu gcacaaagaa      720

ugguuccacg acauaccacu gcccuggcac gcaggcgcag acaccggcac gccacacugg      780

aacaacaagg aagcccuagu agaguucaaa gacgcccacg ccaagcgaca aaccgucgua      840

gugcucggcu cccaagaagg agccguccac acagcucuag ccggagcacu agaagcagaa      900

auggacggag ccaaaggaaa acucucaagc ggccaccuaa aaugccgacu caaaauggac      960

aaauuacgac uaaaaggcgu auccuacucc cuaugcacag ccgcauucac auucacaaaa     1020

auucccgccg aaacgcuaca cggcacaguc acaguggaag uacaauacgc cggcacagac     1080

ggaccaugca aaguaccagc ccaaauggcc gucgacaugc aaacgcugac acccgucggc     1140

agacucauaa cagccaaccc cgucauaacc gaaaguaccg aaaacucaaa aaugaugcuc     1200

gaacucgacc caccauucgg agacagcuac auagucaucg gcgucggaga aaagaaaaua     1260

acccaccacu ggcaccgaag cggcuccaca aucggaaaag ccuucgaagc cacaguacgg     1320

ggagccaaac guauggccgu acucggcgau accgcauggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9610
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729218.1 solE mRNA Design 3 opt

<400>  9610
gggagaaagc uuaccauguc acaaaaaguc auauaucuag ucaugauccu gcuaauagcc       60

ccagccuaca gcauccgcug caucggagua ucgaaccgcg acuucgucga aggcaugagc      120

ggaggcaccu ggguagacgu aguccuagaa cacggcggau gcguuaccgu aauggcacaa      180

gacaagccaa ccguagacau cgagcuaguc accaccaccg uuuccaacau ggcagaagua      240

cgaagcuacu gcuacgaagc aaguauaagc gacauggcgu ccgacagccg gugccccaca      300

caaggcgaag ccuaccucga caaacaaagc gacacacagu acguuugcaa gcggacgcug      360

guggaccgcg gcuggggcaa cggaugcgga uuguucggaa aaggaagccu agucacaugc      420

gcgaaauucg caugcagcaa gaaaaugaca ggcaaaucaa uacagccaga aaaccuagaa      480

uacagaauaa ugcuaagcgu acacggaagu caacacagcg gcaugaucgu caacgacaca      540

ggccacgaga ccgacgaaaa cagagcaaaa guagaaauaa ccccaaacuc gccaagagcc      600

gaagccacac ucggaggauu cggcucgcuc ggacucgacu gcgagcccag aaccggacuc      660

gacuucuccg accuauacua ccucacaaug aacaauaaac acugguuagu acacaaagaa      720

ugguuccacg acaucccacu gcccuggcac gcaggagccg acacaggcac accccacugg      780

aacaacaagg aagcucuagu agaguucaaa gacgcccacg cgaaacguca aaccguagua      840

guacuaggca gccaagaagg cgcagugcac acagcccucg ccggcgcacu agaagcggaa      900

auggacggcg caaaaggcag auuaagcagc ggccaccuaa aaugccgccu aaagauggac      960

aaacuaagac ucaaaggagu aagcuacagc cuaugcacag cagcauucac cuucaccaaa     1020

auacccgccg aaacacuaca cgggacagua acagucgaag uacaguaugc aggcacagac     1080

ggccccugca aaguacccgc acaaauggcc guggacaccc aaacacuaac ccccguaggc     1140

aggcuaauaa ccgccaaccc aguaaucacc gaaagcacag aaaacagcaa aaugaugcuc     1200

gaacuagauc ccccauucgg agacuccuac aucgucauag gagucggcga aaagaaaauc     1260

acucaucacu ggcaccgauc aggcuccacc aucggcaaag cauucgaagc aaccguacgc     1320

ggcgccaaac guauggccgu gcuaggcgac acagccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9611
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU501217.1 solE mRNA Design 3 opt

<400>  9611
gggagaaagc uuaccauguc acaaaaaguc auauacuuag ucaugauacu acucauugcc       60

cccgcauacu ccauacgaug caucggcguc uccaacagag acuucguaga aggcaugagc      120

ggcggcacau gggucgacgu aguccuggaa cacggcggcu gcguaaccgu gauggcccaa      180

gacaaaccaa cuguagacau agaacucguc acaaccaccg uaagcaacau ggccgaaauc      240

cgaagcuacu gcuacgaagc cagcauaagc gacauggcgu ccgacagccg cugcccaacc      300

caaggcgaag ccuaccuaga caaacagagc gacacacaau acgucugcaa acgcacacua      360

guagacagag gcuggggaaa cggcugcgga cuauucggaa aaggaagccu aguaacgugc      420

gcgaaauucg ccugcagcaa aaaaaugaca ggaaaaucaa uccagcccga gaaccuagaa      480

uacagaauua ugcuaagcgu acacgggucc caacacucag gaaugauagu aaacgacacu      540

ggccacgaaa ccgacgaaaa cagagccaaa guagaaauaa cacccaacuc accccgagcc      600

gaagccacac uaggaggcuu cggcucacuc ggacucgacu gcgaaccaag aaccggacua      660

gacuucucgg accucuacua ccucacgaug aauaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccccu cccuuggcac gcuggcgcag acaccggaac accacacugg      780

aacaacaagg aagcacucgu cgaauucaaa gacgcccacg ccaaacgcca aaccguaguc      840

guacucggau cccaagaagg ggcaguacac acagcccugg ccggagcacu cgaagcagaa      900

auggacggcg ccaaaggacg acuaucuagu ggccaccuga aaugucgccu aaaaauggac      960

aaacuccgac ucaaaggcgu aagcuacagc cucugcaccg cagccuucac cuucaccaaa     1020

auaccagcag aaacacuaca cggcacggua accgucgaag uccaguacgc aggaacugac     1080

gggcccugca aagucccagc ccaaauggca gucgacaugc aaacccuuac ccccguagga     1140

cgccucauaa cagccaaccc agucaucacc gaaagcacag aaaacagcaa aaugaugcuc     1200

gaacucgacc cacccuucgg agacucauac auagucauug gcgugggcga aaaaaaaaua     1260

acucaccacu ggcaccgauc gggcucaacu auaggcaaag ccuucgaagc gaccguccgc     1320

ggcgccaaac gcauggccgu acucggcgac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9612
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU761561.1 solE mRNA Design 3 opt

<400>  9612
gggagaaagc uuaccaugag ccaaaaagua auauaccucg ucaugauccu gcucauagcc       60

cccgcuuacu ccauacgcug caucggcguc agcaacaggg acuucguaga aggcauguca      120

ggcggcacau ggguagacgu aguccuagaa cacgggggau gcgucaccgu aauggcccaa      180

gacaaaccaa caguagacau cgaacucguc acgacgaccg ucuccaacau ggccgaagua      240

cgcagcuacu gcuacgaagc aagcauauca gacauggcau ccgacucccg cugccccacc      300

caaggcgaag ccuaccucga caaacaaucc gacacccaau acguaugcaa acgaacccua      360

guagaccgag gcuggggaaa cggcugcgga cuauucggaa aaggcucacu cguaacaugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggaaaaucca uccaacccga aaaccucgaa      480

uaccgcauaa ugcucuccgu ccacggaagc caacacucgg gcaugaucgu aaacgacacc      540

ggccacgaaa ccgacgaaaa ccgcgcaaaa guagaaauaa cacccaacag ccccagagca      600

gaagcaaccc ucggcggcuu uggaucccuc ggacuagacu gcgaaccccg aaccggccuc      660

gacuucagcg aucuauacua ccucacaaug aacaacaaac acuggcuagu ccacaaagaa      720

ugguuccacg acauaccucu gcccuggcac gccggugcag acacaggcac cccgcacugg      780

aacaacaaag aagcccuagu cgaguucaaa gacgcccacg ccaaacgcca aacagucguc      840

guccuaggca gccaagaagg cgcaguccac accgcccuag caggagcccu agaagcagaa      900

auggacggag caaaaggaag auuaucaucc ggccaccuga aaugucgccu aaaaauggac      960

aaacuccgac ucaaaggggu cagcuacagc cucugcacgg ccgcauucac auucacaaaa     1020

aucccagcag aaacacucca cggaaccguc acaguagaag uccaauacgc aggcaccgac     1080

ggccccugca aaguaccagc acaaauggcc guagacaugc aaacccuaac ccccgucgga     1140

cgccuuauca ccgcaaaccc aguaaucacg gaaucaaccg aaaacagcaa aaugaugcua     1200

gaacucgacc cgcccuucgg cgacagcuac auaguuauag gcguaggaga aaagaaaaua     1260

acccaccacu ggcaucgcag cgguucaacc aucggaaaag ccuucgaggc caccguacgc     1320

ggcgcacgcc gcauggccgu acucggcgac acggccuggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9613
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY075937.1 solE mRNA Design 3 opt

<400>  9613
gggagaaagc uuaccaugag ccaaaaagua auauaccuag ucaugauacu acucaucgca       60

cccgccuacu caauccgcug cauaggcguc ucaaaccgag acuucgucga aggcaugagc      120

ggaggaacgu gggucgacgu aguacucgaa cacggcggau gcgugaccgu uauggcacaa      180

gacaaaccaa ccgucgacau agaacucguc acaacaacgg ucucaaacau ggccgaaguc      240

cgcucauacu gcuacgaagc auccauaagc gacaugguau cugacucacg cugcccaacc      300

cagggagaag cguaccuaga caaacaaagc gacacccaau acgucugcaa aagaacccua      360

guggaccgcg gauggggaaa cggcugcggc cuauucggca aaggaagccu aguaacaugc      420

gccaaauucg ccugcagcaa gaaaaugacc ggcaaaucaa uacagcccga aaaccuagaa      480

uacagaauaa ugcuaagcgu ccacggcuca caacacuccg gaaugaucgu aaacgacacc      540

ggacaugaaa ccgacgaaaa ccgagcaaaa gucgaaauca ccccaaacuc acccagagcc      600

gaagccaccc uaggaggauu cggcagccua ggccuagacu gcgaaccacg aaccggacuc      660

gacuucuccg accucuacua ccuaacaaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acaucccccu ccccuggcac gcaggagcag acaccggcac accccacugg      780

aacaacaaag aagcgcucgu cgaguucaaa gacgcacacg ccaaacgcca aaccgucguc      840

guacuaggaa gucaagaagg cgccguacac accgcacucg caggcgcacu agaagcagaa      900

auggacggag ccaaaggccg acucagcagc ggacaccuca aaugccgacu aaaaauggac      960

aagcuacgcc ucaaaggagu cuccuacagc cuaugcacag cagcguuuac auucacgaaa     1020

aucccagcag aaacccucca cggcacaguc acggucgaag uacaauacgc aggcacagac     1080

ggacccugca aaguccccgc gcaaauggcc guagacaugc aaacccugac cccaguaggc     1140

cgccuuauca ccgccaaccc agucaucacc gaauccacag aaaacuccaa aaugaugcuc     1200

gaacuagauc ccccguucgg agacuccuac auaguaauag gcguaggaga aaagaaaauc     1260

acccaccacu ggcacagauc gggcuccaca aucggcaaag cauucgaagc aaccguccga     1320

ggcgccaaac gcauggccgu acucggagac acggcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9614
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014317.1 solE mRNA Design 3 opt

<400>  9614
gggagaaagc uuaccaugag ccaaaaagua auauaccucg ucaugauccu ucucaucgca       60

ccagcauauu ccauacgcug cauuggagua ucaaacagag acuucgucga aggaaugagc      120

ggaggcacgu ggguggacgu gguccuagaa cacgggggcu gcguaaccgu aauggcucaa      180

gacaaaccga caguagacau cgagcuaguc accaccaccg ucucaaacau ggcagaaguc      240

agaaguuacu gcuacgaagc cagcauaagc gacauggcaa gcgacagcag augccccacc      300

caaggcgagg cauaccucga caaacaaagc gacacccaau acguaugcaa acgcacacuc      360

guagaccgcg gcuggggcaa cggcugcgga cuauucggca aaggaagccu cgucacaugc      420

gccaaauucg caugcucaaa gaaaaugacc ggcaaaagca uacagcccga gaaccuggaa      480

uaccgaauaa ugcucagcgu acacggauca caacacucag gcaugaucgu gaacgacaca      540

gggcaugaaa ccgacgaaaa ccgcgccaaa guagaaauca ccccgaacuc cccaagagcc      600

gaagccacac ucggcggcuu cggcucacua ggacucgacu gcgagccaag aaccggccua      660

gacuucucag accuuuacua ccucacaaug aauaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccccu ccccuggcac gcaggcgcag auacaggaac gccccacugg      780

aacaacaagg aagcacuugu agaguucaaa gacgcacacg ccaaacgaca aaccguaguc      840

guacucggaa gccaagaagg agccgugcac accgcccuag caggagcccu cgaagccgaa      900

auggacggag ccaaaggcag acuaucaagc ggacaccuaa aaugccgccu aaaaauggac      960

aaacuacgac ucaagggcgu auccuacagc cuaugcaccg cugcauuuac cuuuacaaaa     1020

auacccgccg aaacacuaca cggcacagua acagucgaag uccaguacgc aggcacagac     1080

gggcccugca aaguaccagc acaaauggca guagacaugc aaacgcuaac gcccguagga     1140

cgccucauca cagccaaccc agucaucacc gaaagcauag aaaacagcaa aaugaugcua     1200

gaacuagauc ccccauucgg agacucauac auagucaucg gcguaggaga aaagaaaauc     1260

acgcaccacu ggcaccgcuc aggaucaacc aucggcaaag ccuuugaagc aacagugcga     1320

ggcgccaaac gcauggccgu acucggagac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9615
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU870645.1 solE mRNA Design 3 opt

<400>  9615
gggagaaagc uuaccauguc gcaaaaagua auauaccuag ucaugauacu gcucauagca       60

ccagccuaca guauacgaug caucggcguc agcaacaggg acuucgucga aggcaugagc      120

ggaggcacau ggguugacgu gguacuagaa cacggcggau gcguaaccgu gauggcacaa      180

gauaaaccaa cgguagacau cgaacuggua acaacaacag uaagcaacau ggccgaagua      240

aggagcuacu gcuacgaagc aagcauaagc gacauggcaa gcgacuccag augcccaaca      300

caaggcgaag ccuaccucga caaacaaagc gacacccaau acgucugcaa acgcacacuc      360

guagauagag gcuggggaaa uggcugcggc cuauucggaa aaggaagccu cgucacgugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggcaaaucga uccaaccaga aaacuuagaa      480

uaucgaauca ugcugagcgu acacgggagc caacacuccg gaaugauagu gaacgacaca      540

ggacacgaaa ccgacgagaa cagggcaaaa guagaaauca caccaaacuc accccgggcc      600

gaagccacac ucggaggauu cggaagccuc ggacuagacu gcgaaccaag aacaggacuc      660

gacuucagug accuauacua ccucacaaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acaucccacu gccauggcac gcgggcgcag acacaggcac accccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacacg ccaaacgcca aaccguaguc      840

guacuaggaa gccaagaagg ggccguacac acagcccugg ccggggcacu cgaagcagaa      900

auggacggag caaaaggcag gcucucguca ggacaucuaa aaugccgccu aaaaauggac      960

aaacucaggc ucaaaggcgu gagcuacagc cuguguacag cagcauucac auucacaaaa     1020

auacccgcag aaacacucca cggcacaguc acgguagaag uacaauacgc aggcacagac     1080

ggcccaugca aaguacccgc gcagauggcc gucgacaugc aaacacuaac acccguagga     1140

cgacuaauaa ccgcaaaccc ggucauaacc gaaagcacgg aaaauagcaa aaugaugcuc     1200

gaacuagacc caccauucgg cgauucauac aucgucaucg gcauaggcga aaagaaaauc     1260

acacaccacu ggcaccgcuc aggcucaaca auaggcaaag ccuuugaagc aaccguacgc     1320

ggggcaaaac gcauggcagu gcuaggggac acagcauggg acuucggcag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9616
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU922923.1 solE mRNA Design 3 opt

<400>  9616
gggagaaagc uuaccauguc acagaaaguc aucuaccuag ucaugauccu ccuaaucgca       60

cucgccuacu caauacgcug cauaggaguc uccaaccgag acuucguaga aggaaugucc      120

ggcggaaccu ggguagacgu aguccucgaa cacgggggcu gcguaacagu cauggcccaa      180

gacaaaccca ccgucgacau agaacucgua accaccaccg ucucaaacau ggccgaaguc      240

cgaucguacu gcuacgaagc cagcaucucc gacauggccu ccgacucccg augcccaacc      300

cagggagaag ccuaccuaga caaacaaagc gacacacaau acguaugcaa acgaacacuc      360

gucgaccgcg gcuggggaaa cgguugcgga cuauucggca aaggaagccu agucacaugc      420

gcaaaauucg caugcucaaa gaaaaugacc ggcaaaucca uccaaccaga aaaccucgaa      480

uaccgaauaa ugcucagcgu ccacggcuca caacacagcg gaaugauagu aaacgacacc      540

ggacacgaaa ccgacgaaaa ccgcgcaaaa gucgaaauca cccccaacuc cccacgcgcc      600

gaagccaccc uaggaggauu cggaucccuc ggacuagacu gcgaacccag aaccggccuc      660

gacuucuccg accucuacua ccucaccaug aacaacaaac acugguuagu ccacaaagaa      720

ugguuccacg acaucccccu ccccuggcac gccggcgccg acacaggaac cccgcacugg      780

aacaacaaag aagcccucgu cgaguucaaa gacgcgcacg ccaaacgaca aaccgucguc      840

guacuagggu cccaagaagg agccguacac acagcacuag ccggcgcccu ugaagcagaa      900

auggacggcg cuaaaggccg acucagcucc ggacaccuca aaugcagacu uaaaauggac      960

aaacuccgcc ucaaaggcgu cagcuacagc cucugcaccg ccgcauucac auucacaaaa     1020

auacccgccg aaacccucca cggcaccguc acaguagaag uccaauacgc cggaaccgac     1080

ggccccugca aaguaccagc ccaaauggca gucgacaugc aaacacuaac cccagucgga     1140

cgacuuauca cggcaaaccc cgucaucaca gaaucaaccg aaaacuccaa aaugaugcuc     1200

gaacucgacc caccuuucgg cgacuccuac aucguaaucg gaguaggcga aaagaaaauc     1260

acacaccacu ggcaccgauc cggauccacg aucggcaaag ccuucgaagc aacaguccgc     1320

ggagcaaaaa gaauggccgu acucggagau accgccuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9617
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU497555.1 solE mRNA Design 3 opt

<400>  9617
gggagaaagc uuaccaugag ccaaaaaguc auauaccuag ucaugauccu ccucauagcc       60

ccagcauacu ccauacgcug caucggagua agcaaccgag acuucgucga aggaauguca      120

ggcggaaccu gggucgacgu cguacuagaa cacggcggau gcguaacagu aauggcccaa      180

gacaaaccaa caguagacau cgaacucgua acaaccacag ucagcaacau ggcagaagua      240

agauccuacu gcuacgaagc cucaauaagc gacauggcau ccgacuccag gugcccaaca      300

caaggcgaag ccuaccucga caaacaaagc gacacacaau acgucugcaa aagaacacuu      360

guagacagag gauggggcaa cggaugcggc cucuucggaa aaggaagccu cguaaccugc      420

gccaaauucg ccugcagcaa aaagaugaca ggaaaaucga uccaacccga aaaccuagaa      480

uaccgcauca ugcucuccgu ccacggcagc cagcacucag gcaugauagu caacgacaca      540

ggacacgaaa cagacgaaaa ccgagccaag guagaaauaa ccccaaacag cccccgagcc      600

gaagcaaccc ucggaggauu cggaucacua ggacuagacu gcgaaccccg aaccggccuc      660

gacuucuccg accuauacua ccuaaccaug aacaacaaac acuggcucgu ccauaaggaa      720

ugguuccacg acauaccccu gcccuggcac gcgggcgcag acacgggcac accccacugg      780

aacaacaaag aagcccucgu cgaguucaaa gacgcccacg caaaacgcca gaccguaguc      840

guacuaggca cccaagaagg agccguccac accgcccugg ccggagcccu cgaagccgaa      900

auggacggag caaaagggcg acucagcagc ggccaccuaa aaugccgccu aaaaauggac      960

aaacuaagac uaaaaggcgu aucauacagc cuaugcacag cagcauucac cuucaccaaa     1020

aucccugcag aaacacucca cggcaccguc acggucgaag uccaguacgc cggcacagac     1080

ggacccugca aagucccagc ccaaauggcc gucgacaugc aaacccuaac acccgucgga     1140

agacuaauaa ccgccaaccc cguaaucacc gaaagcacag aaaauucaaa aaugaugcuc     1200

gaacuagauc ccccguucgg agacuccuac auaguuaucg gcguaggcga gaaaaagauc     1260

acacaccacu ggcacagauc cggcagcaca auaggaaagg ccuucgaagc aacaguccgc     1320

ggagcaaaac gaauggcagu ccucggagac acagccuggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9618
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU312314.1 solE mRNA Design 3 opt

<400>  9618
gggagaaagc uuaccauguc gcaaaaaguc aucuaccuag ucaugauccu ccuaauagcc       60

ccggccuaca guauacgaug caucggcguc agcaacaggg acuucgucga aggcaugagc      120

gggggcacgu ggguagacgu aguuuuagaa cacggcggau gcguaaccgu gauggcacaa      180

gacaagccaa ccguagacau agagcuaguc acaaccacag uuucaaacau ggcagaaguc      240

aggagcuacu gcuacgaagc gagcauaagc gacauggcga gcgacagcag augcccaacu      300

caaggcgaag ccuaccuuga caaacaaagc gacacccagu acguaugcaa aagaacccua      360

gucgaccgag gaugggggaa cgguugcgga cuauucggaa aagggagccu agucacaugc      420

gcgaaauucg ccugcagcaa aaagaugaca ggaaaaagca uccaacccga gaaccuagaa      480

uaucgaauaa ugcucagcgu acacgggagc caacacucag gaaugauagu caacgacaca      540

gggcacgaaa ccgacgaaaa ccgagcgaaa guggagauaa cuccaaacuc acccagagcu      600

gaggcaacac uaggaggcuu cgggucacuu ggacuagacu gcgaacccag aacaggccuc      660

gacuucucgg accuauacua ccuaacaaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauaccacu accuuggcac gcaggagcag acacagguac accacacugg      780

aacaacaagg aagcacucgu agaguucaaa gacgcacaug ccaaacgaca gaccguaguc      840

guacucggga gccaagaagg ggccguacac acggcccugg caggagcacu agaagcagaa      900

auggacggag ccaagggccg acucagcagc ggccaccuca agugcagauu gaaaauggac      960

aagcuacgac ucaaaggcgu aagcuacagu cuaugcaccg ccgcauucac auucacaaaa     1020

aucccagcag aaacgcuaca cggcacaguc accgucgaag uucaguacgc cggaacagac     1080

ggaccaugca aagugcucgc ccagauggca gucgacaugc aaacgcuaac gcccguaggu     1140

aggcucauaa ccgcgaaccc gguaaucaca gaaagcacag aaaacagcaa aaugaugcuc     1200

gagcucgacc cacccuucgg agacagcuac aucgucauag gggugggaga aaagaaaauc     1260

acacaccacu ggcaccgcag cgguucaacc aucggcaaag ccuucgaagc gaccguacga     1320

ggagcuaaaa gaauggccgu acucggagac acagccuggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9619
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY014314.1 solE mRNA Design 3 opt

<400>  9619
gggagaaagc uuaccaugag ccagaaaguc aucuaccuag ucaugauccu gcucauagca       60

ccagcauaca gcauaagaug uaucggaguc uccaacagag acuucgucga aggaaugagc      120

ggaggaaccu ggguagacgu agucuuagaa cacgguggcu gcgucaccgu gauggcacaa      180

gacaaaccga caguagacau agaacucguc acaacaacag uaagcaacau ggcagaagua      240

cgaagcuacu gcuacgaagc guccauaagc gacauggcca gcgacagcag augcccaaca      300

caaggcgaag cauaccuaga caaacaaucc gacacacagu acgucugcaa acgcacccua      360

guagacagag gguggggcaa cggaugcgga cuauucggga aagggagccu agucacaugc      420

gccaaauucg ccugcucaaa gaaaaugaca ggaaaaagca uacaaccaga aaaccuagaa      480

uaccgaauaa ugcuaagcgu acacggaagc caacacagcg gcaugaucgu aaacgacacc      540

ggacacgaaa cagacgaaaa ccgagcaaaa gucgaaauaa cacccaacag cccccgagcc      600

gaggccacac uaggcgguuu cggaagccuc ggacucgacu gcgaaccacg cacagggcua      660

gacuucuccg accucuauua ccuaacaaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccccu acccuggcac gcaggcgcgg acacaggaac cccgcacugg      780

aacaacaaag aagcccuagu cgaguucaaa gaagcccacg ccaaacgaca gacaguaguc      840

guccuaggaa gccaagaagg agccguccac acagcccuag caggagcacu cgaagcagaa      900

auggacgggg ccaaaggacg ccuaagcucc ggacaucuaa aaugccgccu aaaaauggac      960

aaacucagac uaaaaggagu cagcuacagc cuaugcacag ccgccuucac cuucaccaaa     1020

auaccggcag aaacacucca cggcacaguc accgucgaag uccaauacgc aggcaccgac     1080

ggaccaugca aaguacccgc acaaauggcc guagacaugc aaacacuaac cccaguggga     1140

cgacucauaa cugccaaccc agucaucacc gaaucaaccg aaaacagcaa aaugaugcua     1200

gagcucgacc caccauucgg agacagcuac auagucaucg gcgucggcga gaagaaaauu     1260

acgcaucacu ggcacagaag cggcucgacc auaggcaaag ccuucgaagc aacaguacgc     1320

ggagcaaaac gaauggccgu ccucggagac acagccuggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9620
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX447517.1 solE mRNA Design 3 opt

<400>  9620
gggagaaagc uuaccauguc gcaaaaaguc aucuaccuag uaaugauccu gcuaauagca       60

ccagccuaca guaucagaug cauaggaguc ucgaacaggg acuucguaga gggaauguca      120

gggggcacgu ggguagacgu gguacuagaa cacggcggau gcgugaccgu gauggcgcaa      180

gacaaaccga ccguagacau agaacucguc acgacaacgg ucucaaacau ggccgagguc      240

aggagcuacu gcuacgaagc gaguauaagc gacauggcaa gcgacagcag augcccgaca      300

cagggcgagg ccuaccucga caaacagagc gacacccaau acgugugcaa acgcacccua      360

guggaccgag gcuggggcaa cggcuguggc cuguucggga aggggagccu cgucacaugc      420

gcaaaauucg ccugcagcaa gaaaaugaca ggaaaaagca uccagcccga aaaccuggaa      480

uacagaauaa uguuaagcgu gcacggaagc cagcacucag gcaugaucgu aaacgacaca      540

ggccacgaga ccgacgaaaa ccgcgcaaaa guggaaauaa ccccgaacuc gccgagggca      600

gaagccgccc uagggggauu cggcucgcua gggcuagacu gcgagccaag aacaggccug      660

gacuucagcg accuauacua ccuaacgaug aacaacaaac acuggcuagu gcacaaggag      720

ugguuccacg acauacccuu gcccuggcac gcgggagccg acacaggaac gccucacugg      780

aauaacaagg aagcccuagu cgaguucaaa gacgcacacg caaaacgaca gaccguagua      840

gugcuaggga gccaggaagg ggcagugcac acggcucugg ccggagcacu ggaagcggaa      900

auggacgggg ccaaaggcag gcucucaucc gggcaccuga aaugccgccu gaagauggac      960

aaacuaagac ucaaaggcgu cagcuauagc cuaugcacgg ccgcguucac guucacgaaa     1020

auaccagccg aaacgcuaca cggaacgguc acgguagaag uccaguacgc gggcacggac     1080

gggccaugca aagugccggc gcaaauggcc guagacaugc aaacgcucac gccaguggga     1140

aggcuaauaa ccgcgaaccc aguaauaaca gaaagcacag aaaauagcaa gaugaugcuc     1200

gagcucgauc ccccguucgg ggacucauac auagucauag gcgugggcga gaaaaagaua     1260

acgcaccacu ggcacagauc gggaucgacc auaggcaaag ccuuugaagc gaccguacgc     1320

ggggcaaaac gcauggcagu acucggggac acggcauggg acuuugguuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9621
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU729217.2 solE mRNA Design 3 opt

<400>  9621
gggagaaagc uuaccauguc ccagaaaguc auauaccucg uaaugauccu acuaaucgca       60

ccagcauaca gcauaagaug cauaggcguu uccaaccgag acuucguaga aggcaugagc      120

ggcggcacau ggguagacgu cguccucgaa cacggaggau gcguaaccgu cauggcccaa      180

gacaaaccca cagucgacau cgaacugguc ucuacaacag ucuccaacau ggccgaaguc      240

cgaagcuacu gcuacgaagc aaccauaucg gacaucgcau ccgacagccg cugcccgaca      300

caaggcgaag ccuaccucga caagcaaagc gacacacaau acgucugcaa acgaacgcuc      360

gucgaccgag gcugggguaa cggcugcggc cucuucggca aagggucccu cguaacaugc      420

gccaaauucg ccugcucaaa gaaaaugaca ggcaaaucca uccaaccaga aaaccucgaa      480

uacagaauca ugcuauccgu acacggcagc caacacuccg gcaugauugu aaacgacaca      540

ggccacgaaa ccgacgaaaa ccgagcaaaa guagaaauca ccccaaacag cccccgagca      600

gaagccaccc ucggcggauu cggaagccuc ggccucgacu gcgaacccag aaccggacuc      660

gacuucagcg accucuacua ccucaccaug aauaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acaucccccu acccuggcac gcaggcgccg acaccggaac accccacugg      780

aacaacaaag aagcacucgu cgaguucaaa gacgcccacg ccaaacgcca gaccgcaguc      840

guccucggau cacaggaagg cgccguccau acagcccuag ccggcgcccu agaagcagaa      900

auggacggcg cgaaaggccg ccucagcagc ggccaccuaa aaugccgccu caaaauggac      960

aaacuacgcc uuaaaggcgu cagcuauagc cucugcacag cugcauucac cuucacaaaa     1020

auccccgcag aaacccuaca cggcacaguc accgucgaag uacaauacgc cggcacagau     1080

ggcccaugca aagugcccgc ccaaauggcc guggacaugc aaacccucac cccagucggc     1140

agacucauaa ccgccaaccc aguaaucacc gaaucaaccg aaaacuccaa aaugaugcuc     1200

gaacucgauc ccccauuugg agacuccuac auagucaucg gcguaggcga aaagaaaauc     1260

acccaccacu ggcaccgcag cggauccacc auaggcaaag ccuucgaagc cacaguccga     1320

ggagcaaaaa gaauggccgu gcucggagac acagcauggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9622
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY328290.1 solE mRNA Design 3 opt

<400>  9622
gggagaaagc uuaccauguc acaaaaaguc auauaccuag ucaugauccu acuaauagca       60

ccagcguaua gcauacgcug caucggagua agcaacaggg acuucgucga gggcaugagc      120

ggaggcacau ggguggacgu cguacuggaa cacggaggau gcguaacagu gauggcacaa      180

gacaaaccaa ccguagacau agagcuagua acaacaacag ucucgaacau ggccgaaguc      240

agaucauacu gcuacgaagc cagcauaagc gacauggcau ccgacuccag gugcccaacc      300

cagggcgaag cauaccucga caaacaaagc gacacacagu acguaugcaa aagaacccua      360

guggacagag gauggggcaa cggaugcgga cuauucggaa aagggagccu cgucacaugc      420

gcaaaauucg ccugcagcaa gaaaaugaca gggaaaucaa uacagccaga aaaccuagaa      480

uaccgaauaa ugcucagcgu gcacggaagc caacacucag gcaugaucgu aaacgacacc      540

gggcacgaaa cagacgaaaa ccgcgcaaaa gucgaaauaa caccaaacuc acccagagcc      600

gaggccacac uugggggauu cggcagccuc gguuuagacu gcgaaccaag gaccgggcuc      660

gacuucucag accuguacua ccucacaaug aauaacaaac acuggcucgu acacaaagaa      720

ugguuccaug acaucccccu acccuggcac gccggcgcag acacaggcac gccacacugg      780

aacaacaaag aagcgcucgu ggaguucaaa gacgcccacg ccaaacgcca aaccguagug      840

gugcuaggga gccaggaagg agcagugcac acagccuuag ccggcgcccu ggaagcagaa      900

auggacggag caaaaggccg gcucagcagc ggacaccuca aaugccgacu gaaaauggac      960

aaacuacgcc uaaaaggagu gagcuacagc cucugcacag ccgcguucac auucagcaag     1020

auacccgcgg aaacacuaca cggaacagua acagaagaag uccaauacgc aggcaccgac     1080

ggacccugca aggugcccgc acaaauggcc guagacaugc aaacacucac accgguuggc     1140

agacucauca ccgcaaaccc cgugauaacc gaaagcacag aaaacucaaa aaugaugcuc     1200

gaacucgacc cgccauucgg agacucguac auaguaauag gaguaggcga aaagaaaauc     1260

acgcaccacu ggcaccgcag cggaucaaca aucggcaaag ccuucgaagc gaccgucaga     1320

ggagcaaaac gcauggcggu acugggagac acagcauggg acuucggauc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9623
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX087101.3 solE mRNA Design 3 opt

<400>  9623
gggagaaagc uuaccauguc ccaaaaagua auauaccuag uaaugauccu ccuaauagca       60

cccgcauaca gcauccgcug cauagggguc ucgaacaggg acuucguaga aggcaugagc      120

ggaggaacau ggguagacgu agucuuagaa cacggcggcu gcgucaccgu aauggcccaa      180

gacaaaccaa ccguagacau agaacugguc accaccacgg uaucgaacau ggcagaaguc      240

cgaagcuacu gcuacgaagc cagcaucagc gacauggccu cagacagccg augccccacc      300

caaggcgaag ccuaccucga caaacaauca gacacccaau acguaugcaa acgcacccua      360

guagaccgag gcuggggcaa cggcugcggc cuguucggaa aagggagccu ggucacaugc      420

gccaaauucg ccugcuccaa gaaaaugaca ggaaaaucaa uccaacccga aaaccuagaa      480

uaucgcauca ugcuauccgu acacggaucc caacacuccg gaaugauagu aaacgacaca      540

ggacacgaaa ccgacgaaaa ccgcgccaaa guagaaauaa ccccaaacuc ucccagagcc      600

gaagccacac ucggaggauu cggcucccua ggccuagacu gcgaaccccg gaccgggcuc      660

gacuucuccg accucuacua ccucaccaug aacaacaaac acuggcucgu ccauaaagaa      720

ugguuucacg acauaccccu cccauggcac gcaggcgcag acaccgguac accacacugg      780

aacaacaagg aagcgcuagu agaguucaaa gacgcccacg ccaaacgcca aaccguaguc      840

gugcuaggca gccaggaagg agccguacac acagcacugg ccggagcucu cgaagcagaa      900

auggacggag ccaaaggccg gcuaucaagc ggacaccuaa aaugccgccu aaaaauggac      960

aagcuaagac ucaaaggcgu gagcuacagc cuaugcacag ccgcauucac guucaccaaa     1020

auaccugcag aaacccuaca cggaacagua acagucgaac uccaauacgc aggcacggac     1080

ggacccugca aagugcccgc ccaaauggcc guggacaugc aaacgcucac acccguagga     1140

aggcucauua cagcaaaccc agucaucacc gaaagcacag aaaacucaaa aaugaugcua     1200

gaacuugauc ccccauucgg agacucguac auagucaucg gcguagggga gaaaaagauc     1260

acacaccacu ggcaccgauc aggcucaaca aucggcaagg ccuucgaagc caccguccga     1320

ggagcaaaac gaauggccgu acuaggggac acagccuggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9624
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003156.1 solE mRNA Design 3 opt

<400>  9624
gggagaaagc uuaccaugag ccaaaaaguc auauaccugg ucaugauccu ccuaauugca       60

ccagccuaca gcauacguug caucggcauc uccaacagag acuuugucga aggcaugagc      120

ggagggacgu ggguagacgu gguacuagaa cacggaggcu guguaaccgu aauggcccaa      180

gacaaaccaa ccgucgacau cgagcuaguc acuacaacag ucagcaacau ggcagaaguc      240

cgaagcuacu gcuacgaagc cagcauaagc gauauggcga gcgacagcag augcccaacc      300

caaggugaag cauaccuaga caaacaaagc gacacccagu acgugugcaa acgcacacug      360

guagaccggg gcuggggcaa cggcugcggc cuguucggca aaggaucccu agucacaugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggaaaaucua uccaaccaga aaaccuagaa      480

uaucgcauaa ugcuaagcgu acacggaagc cagcacuccg gaaugaucgu aaacgacacc      540

ggacacgaaa ccgacgaaaa ccgagccaaa guagaaauca cccccaacuc gcccagagca      600

gaagcaaccc uuggaggcuu cggaauccua ggacucgacu gcgaacccag gaccggacua      660

gacuucagug accuguacua ccuaacaaug aacaacaaac acuggcucgu ccacaaggaa      720

ugguuccacg acauaccccu cccuuggcac gcaggcgcag acacaggaac gccacacugg      780

aacaacaagg aagcacucgu cgaguucaaa gacgcccacg ccaaacguca aaccgucgua      840

guccucggcu cacaagaagg agccguacac acagcccugg caggagcccu cgaagcagaa      900

auggacggag caaaaggacg ccuaagcagc gggcaccuaa aaugccgccu aaaaauggac      960

aaacuccgac ucaaaggcgu aagcuacagc cuaugcaccg ccgcauucac cuucacaaaa     1020

auacccgcag aaacccucca cggcaccguc accgucgaag uccaauacgc aggaaccgac     1080

ggaccaugca aaguccccgc acaaauggca guagacaugc aaacccucac accaguagga     1140

agacucauca ccgcaaaccc agucaucacc gagagcaccg agaacucaaa aaugaugcuc     1200

gaacucgacc cacccuucgg cgacucuuau auagucaucg ggguaggaga aaagaaaauc     1260

acacaccacu ggcaccgauc gggcuccacc auaggaaaag ccuucgaagc aaccguccgc     1320

ggagccaaac gcauggcugu gcucggagac acggcuuggg acuucggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9625
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU758876.1 solE mRNA Design 3 opt

<400>  9625
gggagaaagc uuaccaugag ccaaaaagua auauaccucg ucaugauacu acuaauagcc       60

ccggcauaca guaucagaug caucgggguc agcaacagag acuucgucga aggcaugagu      120

ggaggcacgu ggguagacgu aguacuagaa cacggaggau gcgucacagu cauggcacaa      180

gacaaaccga ccguagacau agagcuagua acaaccaccg ucagcaacau ggccgaaguc      240

agaagcuacu gcuacgaagc caguauaucc gacauggcca gcgacucgag gugccccacc      300

caaggcgaag ccuaccuuga caaacaaagc gacacacaau acguaugcaa aagaacccua      360

gucgaccgag gcuggggaaa cggaugcggc cucuucggaa agggaagccu agucacaugc      420

gccaaauuug caugcagcaa gaaaaugaca ggaaaaagca uacaacccga aaaccuagaa      480

uaccgcauca ugcuaagcgu ccacggaagc cagcauagcg gcaugauagu aaacgacaca      540

gggcacgaaa ccgacgaaaa ccgagccaaa guagaaauca caccaaacag ccccagagcc      600

gaagccacgc ucggaggcuu cggaagccua ggacucgacu gcgaacccag aaccggacuc      660

gacuucagug accucuacua ccuaacaaug aauaacaaac acuggcuggu ccacaaagaa      720

ugguuccacg acaucccacu acccuggcac gcaggagcgg acacgggcac accacacugg      780

aacaacaaag aagcucucgu agaauuuaaa gacgcccacg caaaacgcca gaccguaguc      840

guccuaggaa gccaagaagg agccguacac accgcccugg caggagcgcu ggaagcggaa      900

auggacggag uaaaaggaag gcuaagcagc ggccaccuca aaugccgccu aaaaauggac      960

aaacuacgcc uaaagggcgu aagcuacagc cuaugcacag ccgcguucac auucacaaag     1020

auaccagccg aaacgcucca cgggacaguc acaguagaag uccaauacgc aggaacagac     1080

ggaccaugca aagugccagc acaaauggcc gucgacaugc aaacccuaac ccccguaggc     1140

cggcuaauaa cagcaaaccc ugucauaacc gaaucgacag aaaacuccaa gaugaugcua     1200

gaacucgauc ccccauucgg cgacaguuac auugucauag gagugggcga aaagaaaaua     1260

acacaccacu ggcaucgaag cggaucaacc aucggcaaag ccuucgaagc caccguacga     1320

ggagccaaac gcauggcagu gcuaggcgac acagcauggg acuucggcag uugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9626
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU926310.1 solE mRNA Design 3 opt

<400>  9626
gggagaaagc uuaccaugag ccaaaaagua auauaccuag ucaugauccu acucauagcc       60

ccagcauaca gcauacgcug cauaggagug uccaaccgag acuucgucga aggcauguca      120

ggcggcacgu ggguagacgu aguacuagaa cacggcgggu gcguaacagu gauggcccaa      180

gacaaaccca cagucgacau cgagcucguc acaaccacag ucuccaacau ggccgaaguc      240

agaucauauu gcuacgaagc aagcaucucc gacauggcaa gcgacagcag augcccaacc      300

caaggagaag ccuaccuuga caaacaauca gacacccaau acgucugcaa acgcacccua      360

gucgaccggg gauggggcaa cggaugcggg cuauucggaa aaggaucccu aguuacaugu      420

gccaaauucg caugcucaaa gaaaaugacc gggaaaucaa uacaacccga aaaccuagaa      480

uaccgcauca ugcugagcgu acacggcagc caacauucag gcaugaucgu aaacgacacc      540

ggacacgaaa cagaugaaaa ccgcgccaaa guggaaauaa cuccaaacuc ccccagagcc      600

gaagccacac ucggaggcuu cggcucgcuc ggacucgacu gcgaacccag aacaggccuu      660

gacuucucag accuauauua ccuuacaaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg auauaccccu acccuggcac gcaggcgcag acaccggcac accccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacacg caaaacgcca aaccgucgua      840

guccucggau cacaagaagg cgccguacac acggcccuug ccggcgcacu agaagcagag      900

auggacggcg ccaaaggccg acuaucaagc gggcaccuga aaugcagacu caaaauggac      960

aaacuccgac uaaagggcgu guccuacuca cuaugcacag cagcauucac auucacaaag     1020

auacccgcag aaacccuaca cggaacggua acagucgaag uacaauacgc cggcgcagau     1080

ggaccaugca aaguacccgc gcaaauggcc guagacaugc aaacacucac gcccgucgga     1140

cgacucauaa cugcgaaccc cgucauaaca gaaagcacag aaaacuccaa aaugaugcua     1200

gaacucgauc ccccauucgg cgacuccuau auagucaucg gcgucggaga gaagaaaaua     1260

acacaccacu ggcaccgcuc cggcuccacu aucggcaaag ccuucgaagc caccguacga     1320

ggagccaaac gaauggccgu ccucggagac accgcauggg acuuuggauc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9627
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU681081.3 solE mRNA Design 3 opt

<400>  9627
gggagaaagc uuaccauguc ccagaaaguc aucuaccugg uaaugauccu acuaauagcc       60

cccgcauaca gcauaagaug caucggagua uccaacagag acuucgucga aggaaugucc      120

ggcggcacau ggguagacgu agucuuggaa cacggaggcu gcguaaccgu cauggcacaa      180

gacaaaccca ccgucgacau cgaacucguc acgaccacag ucucgaauau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcauaagc gacauggcca gcgacagcag augcccaacc      300

caaggcgaag ccuacuuaga caaacagucc gacacccaau acguaugcaa acgcacccuc      360

guagaccgag gcuggggaaa cggcugcggc cuguucggaa aaggaagccu cguaaccugc      420

gcgaaauucg ccugcuccaa gaaaaugacc ggaaaaucaa uccagccuga aaaccucgaa      480

uaccgcauaa ugcucagcgu ccacggaagc cagcauagcg gcaugaucgu aaaugacaca      540

ggacacgaaa ccgacgaaaa ccgcgcaaaa guagaaauaa caccgaacuc ccccagggcc      600

gaggccacac ucggcggcuu cggcucgcuc ggccucgacu gcgaaccaag gaccggacua      660

gacuucagcg accuauacua ccucacaaug aacaacaaac acuggcuagu acacaaagaa      720

ugguuccacg acauaccccu cccauggcac acaggcgccg acaccggcac accccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcccaug ccaaacgcca aaccgugguc      840

guccucggau cacaggaagg cgccguucac accgcucuag caggagcccu agaagccgaa      900

auggacggag ccaagggacg acucucaagc ggacaccuca aaugcagacu caaaauggac      960

aagcuacgac uaaaaggagu aagcuauagc cugugcaccg cagcauucac cuucaccaaa     1020

auacccgcag aaacacucca cggcacggua accgucgaag uacaauacgc aggcaccgac     1080

ggcccaugca aaguacccgc ccaaauggcc gucgacaugc aaacacuaac ccccguaggc     1140

cgacucauaa cagcaaaccc agucauaacc gaaggcaccg aaaauagcaa gaugaugcuc     1200

gaacuggacc cgcccuucgg cgacucguac auagucauag gagugggcga gaaaaagauc     1260

acacaccacu ggcaccgcuc cggcucaacc auaggcaaag ccuucgaagc caccguccga     1320

ggggccaaac gcauggccgu gcucggagac acagccuggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9628
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU744693.1 solE mRNA Design 3 opt

<400>  9628
gggagaaagc uuaccauguc gcaaaaagua auauaccucg ucaugauccu ccucauagcc       60

cccgccuacu ccauacgcug cauaggcguc uccaaccggg acuucgucga aggaaugagu      120

gggggcaccu gggucgacgu aguccuagaa cacggcggau gcguaaccgc aauggcccag      180

gacaagccca ccgucgacau agaacucgua acgacaacag ucuccaacau ggccgaaguc      240

agauccuacu gcuacgaagc aagcaucuca gacauggcaa gcgacagccg augcccaacc      300

caaggcgaag ccuaccuaga caaacaaagc gacacccaau acguaugcaa acgaacccua      360

gucgaccgcg gcuggggcaa cgggugcggc cuauuuggga agggaagccu cgucacaugc      420

gcaaaauuug caugcagcaa aaagaugacc gggaaaagca uccaaccaga aaaucucgaa      480

uaccgcauaa ugcuauccgu ccacggcagc caacacagcg gcaugcuagu aaacgacacc      540

ggacacgaaa ccgacgaaaa ccgcgcaaaa guagaaauaa caccaaacuc cccacgagca      600

gaagcaacac ucggcggcuu cggaagccua ggccuagacu gcgaacccag aaccggacuc      660

gacuucagcg accucuacua ccucacaaug aacaacaaac acuggcuagc ccacaaggaa      720

ugguuccacg acaucccacu acccuggcac gcaggcgcag caacgggaac accacacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcacaug cuaaacgaca aaccgucgua      840

guacuaggaa gccaagaagg cgcaguccac accgcccucg ccggagcacu agaagccgaa      900

auggacggag ccaaggggag acuuucaagc ggccaccuaa aaugucgccu aaaaauggac      960

aaacuacgcc ucaaaggcgu cagcuacagc cucugcacag ccgcauucac auucaccaaa     1020

auaccugcug aaacagucga cggaacagua acagucgaag gacaauacgg aggaacugac     1080

ggccccugca agguaccagc acaaauggcc gucgacaugc agacgcuaac gccaguagga     1140

cgacucauaa ccgccaaccc agucauaacc gaauccacag aaaacagcaa aaugaugcuc     1200

gaacuagacc cacccuucgg ggacuccuac auagucauag gagucggcga aaagaaaauc     1260

acccaccacu ggcaccgcag cggcucaacc auaggcaaag cauucgaagc gacaguacgc     1320

ggagcaaaac gcauggccgu acuaggagac acagccuggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9629
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EU545988.1 solE mRNA Design 3 opt

<400>  9629
gggagaaagc uuaccauguc ccaaaaggug auauaccuag ucaugauccu ccuaauagcc       60

ccagccuaca gcauacggug caucggaguc uccaacagag acuucgucga aggcaugagc      120

ggaggaacau ggguagacgu aguccuagaa cacggcggau gcguaacagu gauggcgcaa      180

gacaaaccag ccguagacau agaacugguc acaacaacgg ucagcaacau ggcagaaguc      240

agaagcuacu gcuacgaagc aagcauaagc gacauggcga gcgacagcag augcccaacc      300

cagggcgaag cauaucucga caaacaauca gacacacagu acguaugcaa gagaacgcuc      360

guagaccgag gcuggggcaa cggaugcggc cuguucggaa aggggagccu cgucacgugc      420

gcgaaauucg cgugcagcaa gaaaaugacc gggaaaucaa uacagccaga gaaccuggaa      480

uaccgaauaa ugcucagcgu ccacggcagc caacacagcg gcaugaucgu caacgacaca      540

ggacaugaaa ccgacgaaaa ucgcgcaaaa gucgaaauca caccgaacuc gcccagagcc      600

gaagccacac ucgggggauu cgguagccua ggacucgacu gcgagcccag gacaggacuc      660

gacuucucgg accuguacua ccuaacaaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauaccccu acccuggcac gcaggcgcag acacaggcac accccacugg      780

aacaacaaag aagcccuagu ggaguucaaa gacgcacaug caaaacgaca aaccguagua      840

gugcuaggaa gccaggaagg agccguacac acagcccugg ccggagcauu ggaagccgaa      900

auggacggag caaaaggcag gcucuccagc gggcaccuga aaugccgccu gaaaauggac      960

aaacuacgac ucaaaggcgu guccuacagu cucugcaccg cagcguucac auucacaaaa     1020

auacccgcag aaacgcucca cggcacgguc acggucgaag uccaauacgc uggaaccgac     1080

ggacccugca aaguaccagc gcaaauggcc guggacaugc agacgcucac accaguggga     1140

cggcucauaa cagcgaaccc agucauaaca gaaagcacag aaaacaguaa aaugaugcuc     1200

gaacuagacc cgcccuucgg ggacuccuac auaguaaucg gcguaggcga aaagaaaaua     1260

acgcaccacu ggcaccgcuc cggcaguacc auaggaaaag cauucgaagc gacaguacga     1320

ggagccaagc gcauggcggu acugggagac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9630
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY007221.1 solE mRNA Design 3 opt

<400>  9630
gggagaaagc uuaccaugag ccaaaaaguc aucuaccuug uaaugauccu ccuaauagcc       60

ccagcguaca gcauccgcug caucggcgua aacaacaggg acuucguaga aggcaugucc      120

ggaggcaccu ggguagacgu cguacucgaa cacgguggau gcguaaccgu aauggcccaa      180

gacaaaccaa ccguagacau cgagcuaguc acgaccacag uaagcaacau ggcagaaguc      240

aggagcuacu gcuacgaagc aagcaucagc gacauggcaa gcgacagccg cugcccaacc      300

caaggagaag cguaccucga caaacaaagc gacacacaau acgucugcaa aagaacccuc      360

guggaccgag gcuggggcaa cggcuguggc cuauucggca aagggagccu agucacaugc      420

gccaaauucg caugcagcaa gaaaaugacc ggaaaaagca uccaacccga aaaccuagaa      480

uaccgaauaa ugcugagcgu ccacggaagc caacacucag gcaugaucgu caacgacacc      540

ggccacgaaa cagacgaaaa cagagcaaaa guagaaauca ccccaaacag ccccagagcc      600

gaagcaacac uaggcggcuu cggaagccug ggacuggacu gcgagccccg aaccggacua      660

gacuucucag accuauacua ccucacaaug aauaacaaac acuggcuagu acacaaagaa      720

ugguuccacg acauaccacu gccauggcac gccggcgcgg acaccggaac accccacugg      780

aacaacaaag aagcacucgu agaguucaaa gacgcccacg ccaaacgcca aaccgucgua      840

guacuaggca gccaagaagg agccguacac acagcccugg ccggagcacu cgaagccgaa      900

auggacgggg ccaaaggccg acucuccagc gggcaccuca aaugccgccu caaaauggac      960

aaacuacgac ucaaaggcgu aagcuacagc cucugcacag ccgcauucac auucaccaaa     1020

auacccgccg aaacccuaca cgguacagug acagucgaag uacaauacgc aggcacggac     1080

gggcccugca aaguacccgc acagauggcc guagacaugc aaacacuaac accagucggc     1140

agacucauca cagccaaccc cguuaucacc gaaagcacag aaaacagcaa aaugaugcuc     1200

gaacuagacc caccauuugg cgacagcuac auagucauug gagucggaga aaagaaaauc     1260

acacaccacu ggcacagauc cggcagcaca aucggaaaag ccuucgaagc aaccguccga     1320

ggcgcgaaac gaauggcagu acuaggagac acagcauggg acuucgguag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9631
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY003152.1 solE mRNA Design 3 opt

<400>  9631
gggagaaagc uuaccauguc ccaaaaagua auauaccuag uaaugauauu acucauagca       60

ccggccuaca gcauccgcug uauaggggua uccaacagag acuucgucga gggcaugagc      120

ggcggcacau gggucgacgu cguacuagag cacggcggcu gcguaacagu cauggcacaa      180

gacaaaccaa ccguggacau cgagcuaguc accaccacag ucucaaacau ggccgaaguc      240

agaagcuacu gcuacgaagc gagcaucagc gacauggcaa gcgacagcag augcccaacc      300

caaggcgagg cuuaccucga caagcagagc gacacccaau acgugugcaa acgcacgcua      360

guagacagag gcuggggaaa uggaugcgga cuuuucggaa aaggaagccu aguuacgugc      420

gcaaaauucg ccugcagcaa gaaaaugacc gggaaaucaa uccaacccga aaaccuagaa      480

uaccgaauaa ugcuaagcgu acacggcagc caacacucgg gaaugauagu aaacgacaca      540

ggacacgaga cagacgaaaa cagagccaaa guagaaauaa caccgaacuc acccagggcc      600

gaggcaacac ucggaggcuu cggcucgcua gggcucgacu gcgagcccag aaccggccua      660

gacuucuccg accuauacua ccucacaaug aauaacaaac acuggcuagu acacaaggaa      720

ugguuccacg acauaccacu gcccuggcac gcaggcgcag acacaggaac accccacugg      780

aacaacaagg aagcccuagu cgaguucaaa gacgcacacg caaagcgaca aaccguaguc      840

guccuuggaa gccaagaagg agccguacac acagcccucg caggggcccu ggaagcagaa      900

auggacggag ccaaaggcag acuauccagc ggacaccuga agugccgacu caaaauggac      960

aagcucaggc ucaagggagu cuccuacagc cuaugcgcag ccgccuucac auucacaaaa     1020

auacccgcgg aaacgcucca cggcacagua acggucgaag uccaguacgc aggcacagac     1080

ggacccugua aaguacccgc gcaaauggca gucgacaugc aaacacuaac accagucggc     1140

aggcucauaa ccgccaaccc ggucauaacc gagaguacag aaaacagcaa aaugaugcuc     1200

gaacucgauc ccccauucgg agacagcuac auaguaauag gaguaggcga aaagaaaauc     1260

acccaccacu ggcacagauc aggcucaaca auaggcaaag cauucgaagc aaccguacgc     1320

ggagcaaaac gaauggccgu ccuaggggac accgccuggg acuuugguuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9632
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX694533.2 solE mRNA Design 3 opt

<400>  9632
gggagaaagc uuaccauguc acaaaaagua aucuaccucg uaaugauccu acuaaucgca       60

cccgccuaca gcauacgaug caucggagua ucaaaccgcg acuucgucga agguaugucc      120

ggcggaacau ggguagacgu cgucuuagaa cacggagggu gcguaacagu cauggcccaa      180

gacaaaccaa caguagacau agagcucgua acaaccaccg uaagcaacau ggcagaaguc      240

cgcagcuacu gcuacgaggc cagcauauca gacauggccu ccgacucacg augcccaaca      300

caaggagaag cauaccuaga caaacaaagc gacacccaau acgucugcaa aagaacacug      360

gucgacagag gauggggcaa cggaugcggc cuauucggaa aaggcagccu aguaaccugc      420

gcaaaauucg ccugcagcaa gaaaaugacc ggcaaaucca uacaaccaga aaaucuagaa      480

uaccgaauaa ugcuauccgu ccacggaagc caacacuccg gcaugaucgu caacgacauc      540

ggccacgaaa ccgacgaaaa ccgagccaaa gucgaaauca cccccaacuc accccgagca      600

gaagccacac ucggcggcuu cggaucccuc ggacuagacu gcgaacccag aacaggccuc      660

gacuucagcg accucuacua ccuaacaaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acauaccacu gcccuggcac gcaggcgccg acacaggcac gccccacugg      780

aacaacaaag aggcacucgu ggaguucaaa gacgcccacg ccaaaagaca aacaguagua      840

guacucggca gccaagaagg agccguccac acagcccucg caggagcccu cgaagccgaa      900

auggacggag ccaaaggcag acucuccagc gggcaccuga aaugccgccu caaaauggac      960

aaacuacgcc ucaaaggcgu cagcuacagc cucugcacag cagccuucac cuucaccaaa     1020

auccccgccg aaacacucca cggaaccgua acaguagaag uccaauacgc cggcacagac     1080

ggacccugca aaguacccgc acaaauggcc gucgacaugc agacccucac gcccguagga     1140

agacucauca ccgcaaaccc cgucauaacc gaaagcacag aaaacagcaa gaugaugcua     1200

gaacucgacc cacccuucgg agacagcuac aucguaauag gagugggaga caagaaaauc     1260

acacaucacu ggcaccgcag cggcucaaca auaggcaaag cauuugaagc cacaguacgc     1320

ggggccaaaa gaauggccgu ccuaggggac accgccuggg acuucggauc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9633
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX601167.1 solE mRNA Design 3 opt

<400>  9633
gggagaaagc uuaccaugag ucaaaaaguc auauaccucg ucaugauccu gcuaaucgcc       60

cccgccuaca gcauacgcug uaucggagug agcaacaggg acuucguaga aggaaugagu      120

ggaggcaccu gggucgacgu aguacuagaa cacggaggau gcguaaccgu aauggcacaa      180

gacaaaccaa ccguagacau cgagcuaguc acaaccacag ucucaaacau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcaucagc gacauggcaa gcgacagccg augcccaaca      300

caaggagaag cauaccuaga caaacaaagc gacacccaau acgugugcaa acgcacccua      360

gucgaccgag gauggggcaa uggaugcggc cucuucggaa aaggaucccu agucacaugc      420

gcgaaauucg caugcagcaa gaaaaugacu ggaaaaucaa uccagccaga aaaccuagaa      480

uaccgaauca ugcucucggu acacggcagu caacacagcg guaugauagu gaacgacacc      540

ggacacgaaa ccgaugaaaa cagagccaaa gucgaaauaa ccccaaacuc ccccagagcc      600

gaagccacac uaggaggcuu cggaucgcua ggacuugacu gcgaaccaag aaccggacua      660

gacuucuccg accuauacua ucuaacaaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg auauaccacu gccauggcac gcaggagcgg acacaggaac accccacugg      780

aacaacaaag aagcccuagu agaguucaaa gacgcccacg ccaaacgaca aaccgucguc      840

guacuaggca gccaggaagg agccguacac acagcccucg cgggcgcccu agaagccgaa      900

auggacggag caaaaggcag acuaucguca ggccaccuca aaugccgccu gaaaauggac      960

aaacuaaggc ucaaaggagu gucauacagc cucugcacag ccgcguucac auucacaaaa     1020

auaccagcag aaacacucca cggaacagua acaguagaag uccaauacgc aggaacagac     1080

ggacccugca aaguccccgc ccaaauggcc guagacaugc aaacccucac gccagucggc     1140

agacugauaa ccgcaaaccc aguaaucaca gaaagcacag aaaacucaaa aaugaugcuc     1200

gaacuagacc caccauucgg cgacagcuau auaguaauag gcgucggaga caagaaaauc     1260

acacaccacu gguaccgcuc aggcuccacc aucggaaaag ccuucgaagc caccguacga     1320

ggagcaaaac gcauggcagu gcuaggggac acggccuggg acuucggaag cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9634
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KY288905.1 solE mRNA Design 3 opt

<400>  9634
gggagaaagc uuaccaugag ccaaaaagua auauaccuag uaaugauccu acuaauagcc       60

cccgcauaca gcauccgcug cauaggggua uccaacaggg acuucgucga aggcaugagc      120

ggaggcacau gggucgacgu gguccuagag cacggaggcu gcguaaccgu aauggcacaa      180

gacaaaccaa ccgucgacau cgaacugguc accaccacag uuagcaacau ggccgaaguc      240

agaagcuacu gcuacgaagc aagcaucuca gacauggcaa gcgacucccg gugccccaca      300

caaggagaag cauaccuaga caaacagagc gacacccaau acguaugcaa acgcacacuc      360

gucgaccgag gcuggggcaa cggcugcgga cuauucggaa aaggaagccu cgucacaugc      420

gcaaaauuca caugcagcaa aaaaaugaca ggaaagagca uccaaccaga aaaccuagaa      480

uaccgcauaa ugcuaagcgu acacgguagc caacacucag gcaugauagu caacgacacc      540

ggccacgaaa ccgacgaaaa ccgagccaaa guagagguca cccccaacag cccacgagcc      600

gaagcaaccc uaggcggcuu cggcagcuua ggccucgacu gcgaaccccg caccgggcua      660

gacuucagcg accuauacua ccuaaccaug aacaacaaac acuggcucgu gcacaaagaa      720

ugguuccacg acauacccuu accauggcac gccggcgcgg acacaggcac accccacugg      780

aacaacaaag aagcccucgu cgaauucaaa gacgcucacg ccaaacgcca aacaguagua      840

guacuaggca gccaggaagg cgcgguacac accgcccuag ccggagcccu agaagccgaa      900

auggacgggg ccaaaggccg acucuucagc ggacaccuga aaugccggcu gaaaauggac      960

aaacucagac uaaaaggcgu cagcuacagc cuaugcacag cagccuucac auuuacaaaa     1020

guacccgcag aaacacucca cggcaccguc acagucgaag uacaauacgc aggcacagac     1080

ggcccaugca aaguaccagc acaaauggcc guagacaugc aaacacucac accagugggc     1140

agacucauca ccgccaaccc aguaauaacc gaaagcaccg aaaacuccaa aaugaugcua     1200

gaacucgacc caccauucgg cgacuccuac auaguaauag gcgucggcga caaaaaaauc     1260

acacaccacu ggcaucgaag cggcuccaca aucggcaaag cauucgaagc aaccguaaga     1320

ggagccaaac gaauggcugu acucggcgac accgcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9635
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF268950.1 solE mRNA Design 3 opt

<400>  9635
gggagaaagc uuaccauguc gcaaaaaguc aucuaccuag uaaugauccu ccucauagcc       60

ccagcuuaca gcaucagaug uaucggagua uccaacagag acuucgucga aggcaugagc      120

gggggcacau gggucgacgu aguccuagag cacggcgggu gugucacugu aauggcgcaa      180

gacaaaccaa caguagacau agagcuagua acaaccaccg ucucaaacau ggccgaaguc      240

cgaagcuacu gcuaugaagc cagcauaagc gacauggcaa gcgacagcag augcccgaca      300

caaggcgaag ccuaccuaga caaacaaagc gacacccaau acguaugcaa acgcacccua      360

guagaccggg gauggggaaa cggcugcggc cuauucggga aaggaagccu aguaacaugc      420

gccaaauuca caugcagcaa gaaaaugacc gggaaaucaa uccaacccga aaaccuagaa      480

uaccgcauaa ugcugagcgu gcacggaagc caacacucag gcaugauagu aaacgacauc      540

ggccacgaaa cagacgaaaa ccgagcgaaa guagaaguga caccgaacuc acccagggca      600

gaagccacgc ucggaggcuu cgguucguua ggacuagacu gcgagcccag aacaggacuc      660

gacuuuucgg accuguacua ccuaaccaug aacaacaaac acuggcuggu acacaaagaa      720

ugguuccacg acauaccccu gccauggcac gcaggagcag acacuggcac accccacugg      780

aacaacaaag aagcacucgu ggaguucaaa gacgcccacg ccaaacgaca aaccgucguc      840

gugcucggaa gucaagaagg agcagugcac accgcccucg ccggagcacu agaagcggaa      900

auggacgggg ccaaaggccg ccucuucagc ggacaccuca aaugcaggcu gaaaauggac      960

aaacuaagac ucaagggagu aagcuacagc cucugcaccg cugcauucac cuucacgaaa     1020

guacccgcgg aaacgcuaca cggaacaguu acggucgaag uccaguacgc aggcacggac     1080

gggcccugca aagugcccgc acagauggcc guugacaugc aaacacucac cccagucggc     1140

agacucauaa cugcaaaccc ggucaucacc gaaaguacgg aaaacagcaa gaugaugcua     1200

gagcuagauc ccccauucgg ggacuccuac auagucaucg gcgugggcga caagaaaauc     1260

acgcaccacu ggcaccgauc gggcucgacc aucggcaaag ccuuugaagc aaccgugcga     1320

ggagccaaac gcauggccgu ccucggcgac acagccuggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9636
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383116.1 solE mRNA Design 3 opt

<400>  9636
gggagaaagc uuaccauguc ccaaaaagua auauaccucg ucaugauacu ccucauagcc       60

cccgcauaca gcauacgcug caucgggguc uccaacagag acuucgucga aggaaugagc      120

ggaggcacau ggguggacgu aguccuggaa cacggaggau gcguaaccgu aauggcccaa      180

gacaaaccaa ccgucgacau agagcuaguu acaaccaccg ucuccaacau ggccgaaguc      240

cgcuccuacu gcuacgaagc cagcauaagc gacauggcca gcgacagcag augcccgacc      300

caaggcgaag ccuaccuaga caaacaaagu gacacccaau acgucugcaa acgaacacuc      360

guagaucgcg gcuggggcaa cggcugcggu cuguucggaa aaggaagccu aguaaccugc      420

gccaaauuca caugcagcaa gaaaaugacc ggaaaaucaa uccagcccga aaaccucgaa      480

uaccgcauaa ugcuauccgu acacgguagc caacacuccg gcaugaucgu caacgacauc      540

ggacacgaga cagacgaaaa ccgcgccaaa guagaaguaa ccccaaacuc ccccagggcc      600

gaagcaacac ucggaggcuu cggaucccua ggacuagacu gcgaacccag aaccggccuc      660

gauuucagcg accuauacua ccucacgaug aacaacaaac acuggcucgu acacaaagaa      720

ugguuccacg acauaccccu acccuggcac gccggcgcag acaccggcac accccacugg      780

aacaacaaag aagcacucgu agaguucaaa gacgcacacg caaaacgcca aaccguagua      840

guccuaggca gccaagaagg cgccguacac accgcccugg ccggagcgcu agaagcagaa      900

auggacggag ccaaaggcag acuauucagc ggccaccuaa aaugccgacu aaaaauggac      960

aaacucagac ucaaaggagu aagcuacagc cuaugcaccg ccguaugcac cgccgccaaa     1020

guccccgcag aaacacuaca cggcacaguc acaguagaag uccaauacgc aggcacggac     1080

ggccccugca aagugccagc ccaaauggcc guagacaugc aaacucugac accagucggc     1140

agacucauaa ccgccaaccc aguaauaaca gaaagcacgg aaaacucaaa aaugaugcuu     1200

gaacucgacc cgcccuucgg agacucauac auagucauag gaguaggcga caagaaaauc     1260

acacaccacu ggcaccggag cggcuccacu auaggcaaag cauucgaagc aaccguaaga     1320

ggagccaaac gaauggccgu acuaggggac acagcauggg acuucggcuc cugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9637
<211>  1564
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KU963574.2 solE mRNA Design 3 opt

<400>  9637
gggagaaagc uuaccauguc gcaaaaaguc auauaccugg ucaugauccu gcuuaucgca       60

cccgcuuacu ccauccgcug caucggaguc ucgaacaggg acuucgucga aggcaugagc      120

ggaggcacau ggguagacgu aguccuagaa cacggcggcu gcgugaccgu cauggcacaa      180

gacaaaccga caguagacau agaacucguc acaaccacag ucucgaacau ggcagaaguu      240

agaaguuacu gcuacgaagc cagcauaagc gacauggcaa gcgacagcag augcccaacc      300

caaggggaag cauaccugga caaacaaagc gacacccaau acgucugcaa acgcacgcua      360

guagacaggg gauggggcaa cggaugcgga cuguucggga aaggaagccu agucacaugc      420

gcgaaguuca caugcagcaa gaaaaugaca ggaaaaucca uacaacccga aaaccuagaa      480

uaccgaauaa ugcugagcgu gcacggaagc caacacagcg gcaugauagu aaacgacgaa      540

aaccgcgcga aaguagaagu aaccccaaac agcccaaggg ccgaggcaac gcuaggagga      600

uucggaagcc uagggcucga cugcgagccc agaaccggac ucgacuucuc ggaccuauac      660

uaccuaacga ugaacaacaa acacuggcua guacacaaag aaugguucca cgacauaccg      720

cuccccuggc acucgggagc agacaccgaa acgccacacu ggaacaacaa agaagcgcua      780

guagaguuca aagacgccca cgccaagcga cagacaguag ucguacucgg gagccaggaa      840

ggagccguac acacagcccu cgccggagcc cucgaagcag agauggacgg agccaaaggc      900

cgacuaucgu ccggccaccu aaaaugccgc cugaaaaugg acaaacucag gcuaaaaggc      960

gugagcuaca gccugugcac cgccgcauuc acauucacaa aagugcccgc agaaacacuc     1020

cauggaacag ucacagucga aguccaauac gcaggaaggg acgggcccug caaaguaccc     1080

gcgcagaugg caguagacau gcaaacgcua acccccgucg gcagacucau aacagcgaac     1140

ccggucauaa ccgaaagcac agaaaacagc aaaaugaugc uagaauuaga ucccccauuc     1200

ggggacucau acaucgucau aggcguaggc gacaagaaaa uaacacacca cuggcacaga     1260

ucaggcucca uaauaggcaa agccuucgaa gcaacagugc gaggagccaa acgcauggca     1320

guacuaggag acaccgccug ggacuucggc ucaugaggac uaguuauaag acugacuagc     1380

ccgaugggcc ucccaacggg cccuccuccc cuccuugcac cgagauuaau agaucuaaaa     1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     1560

aauu                                                                  1564


<210>  9638
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383118.1 solE mRNA Design 3 opt

<400>  9638
gggagaaagc uuaccauguc acaaaaggua auauaccuag ucaugaucgu acucauaguc       60

ccggcauaca gcauaagcug uaucggagua ucaaaccgag accugguaga gggcaugagc      120

gggggcacau ggguggacgu gguacuggaa cauggcggau gcguaaccga aauggcgcaa      180

gacaaaccga ccguggacau agagcuagua acaaugaccg ucucgaacau ggcagaaguc      240

agaagcuacu gcuacgaagc aagccugagc gacauggcaa gcgcaaguag gugcccgacc      300

caaggagaac caucacuaga caaacaaagc gacacccaga gcguaugcaa gagaacgcua      360

ggggacagag gcuggggaaa cggcugcgga aucuucggaa aaggaucccu agucacgugc      420

uccaaauuua caugcugcaa gaaaaugcca gggaaaagca uccagccaga gaaccucgaa      480

uauagaauaa ugcuacccgu gcacggcagc cagcacucag gaaugaucgu gaacgacauc      540

ggacacgaga ccgacgaaaa ccgcgcaaaa guggaaguaa ccccaaacuc acccagagca      600

gaagccacgc ucggaggauu cggaagccuc gggcucgacu gcgaaccccg aaccgggcuc      660

gacuucucag acuuauacua ccuaacaaug aacaacaagc acuggcucgu acacaaagag      720

ugguuccacg acauaccccu gccauggcac gcgggagcag acacaggcac gccgcauugg      780

aacaacaagg aggcacucgu agaguucaaa gacgcgcacg caaagcgaca aacagucgua      840

gugcucggaa gccaagaagg ggccguccac acagccuuag ccggggcccu cgaagcagaa      900

auggacgggg caaagggccg gcucuuuagc ggccaccuaa aaugccgccu aaaaauggac      960

aagcuaagac uaaagggcgu aagcuacagc cugugcacag ccgcauucac auucacaaaa     1020

guacccgcag aaacgcucca cggcacaguc acagucgagg uccagagcgc gggaacggac     1080

ggacccugca aaguaccagc gcaaauggcc guagacaugc aaacacucac ccccguggga     1140

aggcuaauaa cagcaaaccc gguaauaacc gaaagcacag aaaacagcaa gaugaugcuc     1200

gaguuagacc caccauucgg ggacucauac auaguuauag gcguaggcga caagaaaauc     1260

acgcaccauu ggcacagauc cggaaguacc aucggcaaag ccuucgaagc aaccgugcgc     1320

ggggccaaga gaauggcagu gcucggagau accgcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9639
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KX520666.1 solE mRNA Design 3 opt

<400>  9639
gggagaaagc uuaccaugcg acaaaaaguc auuuaccuag uaaugauacu ccuaauagcc       60

ccagccuaca gcauuagaug caucggggua ucaaacaggg acuucguaga aggaaugagc      120

ggcggaaccu ggguagacgu cguccucgaa cacggcggcu gcguaaccgu aauggcacaa      180

gacaaaccga ccguagacau agaacucguc accaccacag ucucaaacau ggcagaaguc      240

cggagcuacu gcuacgaggc uucuauaagc gacauggcaa gcgacagcag augcccaacc      300

caaggcgaag cauaccuaga caaacaaagc gacacccagu acguaugcaa aagaacccuc      360

guagaccgag gauggggcaa cggcugcggc cuguucggaa aaggaagccu gguaacaugc      420

gcaaaauuug ccugcagcaa aaagaugaca gggaaaucca uccaacccga aaaccuagaa      480

uacagaauaa ugcucagcgu acauggaagc caacauagcg gcaugaucgu caacgacaca      540

ggacacgaga cagacgaaaa ccgcgcaaaa guagaaauca ccccaaacuc acccagggcc      600

gaggcaacac ucggcggauu cggcagccuc ggccucgacu gcgaacccag aaccggccuc      660

gacuucucag accuguacua ucuaaccaug aacaacaaac acuggcucgu ccacaaagaa      720

ugguuccacg acaucccacu gcccuggcac gccggcgccg acacaggaac accacacugg      780

aacaacaaag aagcccuagu cgaguucaaa gacgcacacg caaaacgaca aacagucguc      840

guccucggca gccaagaagg agcaguccac acggcccugg caggagcccu cgaagcagaa      900

auggacggag ccaaaggcag gcucucauca ggacaccuaa aaugccgccu uaaaauggac      960

aaacuccgac ugaaaggagu auccuacagc cucugcacag ccgcauucac cuucacaaaa     1020

auaccagcgg aaacacugca cggcacaguc accgucgaag uccaguacgc aggcaccgac     1080

ggccccugca aaguaccagc acaaauggcg guggacaugc aaacccuaac cccaguaggc     1140

cgacuaauaa ccgcgaaccc agucauaacc gagagcaccg aaaacaguaa aaugaugcuc     1200

gagcucgacc caccauucgg agacagcuac auaguaauag gcgucgguga aaagaaaauc     1260

acacaccacu ggcaccgcuc gggcucaacc aucggcaaag ccuucgaagc aacuguacgc     1320

ggagccaaac gcauggcagu acuaggagac acagcauggg acuucggcuc augaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9640
<211>  1582
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  KF383120.1 solE mRNA Design 3 opt

<400>  9640
gggagaaagc uuaccaugag ccaaagaguc aucuaccugg uaaugauccu acuaauagcc       60

ccagccuaca gcauccgaug caucgggguc ucgaacagag acuucgucga aggcaugagc      120

ggaggcacau ggguagacgu agugcuggaa cacggcggau gcguaaccgu aauggcacaa      180

gacaaaccaa ccguagacau agagcuaguc acaacaacag ucucaaacau ggccgaaguc      240

aggaguuacu gcuacgaagc aagcauaagc gacauggcca gcgacagccg augcccaacc      300

caaggcgaag cauaccuaga caaacaaagc gacacccaau acgugugcaa acgcacgcua      360

gucgacagag gauggggcaa cgggugcggc cucuucggga aaggaagccu agucacgugc      420

gcaaaauuca caugcagcaa gaagaugacc ggaaaaucaa uccaacccga aaaccuagaa      480

uaccgcauaa ugcugagcgu ccacggaagc caacacucag gcaugaucgu caacgacauc      540

ggacacgaga ccgacgaaaa ccgagcgaaa guggaaguaa ccccaaacuc accaagggcc      600

gaggccaccc ucggaggcuu cggaucgcuc gggcuagacu gcgaacccag aaccggacuc      660

gacuucucag accucuacua ccucacgaug aacaacaaac auuggcucgu gcacaaagaa      720

ugguuccacg auaucccacu gcccuggcac gcaggcgcgg acacaggaac accccacugg      780

aacaauaagg aagcacuugu agaguucaaa gacgcccacg ccaaacgcca gaccguaguc      840

guacuaggaa gccaagaagg agccgugcac accgcccugg caggagcacu ggaagcagaa      900

auggacggag ccaaaggccg acucuucagc ggacacuuga aaugccgcau aaaaauggac      960

aaacucaggc ucaaaggcgu gagcuacagc cuaugcacug ccgcauucac auucuccaag     1020

guaccagcag aaacacucca cggcaccguc acgguagaag uccaauacgc aggccgagac     1080

gggccaugca aagugcccgc acaaauggcc guagacaugc aaacgcucac acccguaggc     1140

aggcucauaa cagcgaaccc aaucauaaca gaaaguacag aaaacagcag aaugaugcua     1200

gaacucgacc caccguucgg ggacuccuac auagucaucg gcgugggcga caagaaaaua     1260

acacaccacu ggcauagauc gggcucaacc auaggcaaag ccuucgaagc gacagugcga     1320

ggagcaaaaa gaauggccgu acuaggagac acagcauggg auuucggcuc gugaggacua     1380

guuauaagac ugacuagccc gaugggccuc ccaacgggcc cuccuccccu ccuugcaccg     1440

agauuaauag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1500

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     1560

ucuuuucaga gccaccagaa uu                                              1582


<210>  9641
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant

<400>  9641

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
        595                 600                 605             


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe 
625                 630                 635                 640 


Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln 
                645                 650                 655     


Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn 
            660                 665                 670         


Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe 
        675                 680                 685             


Leu Ser Thr Ala Val Ser Ala 
    690                 695 


<210>  9642
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant

<400>  9642

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Thr Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
        595                 600                 605             


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe 
625                 630                 635                 640 


Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln 
                645                 650                 655     


Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys Asn 
            660                 665                 670         


Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe 
        675                 680                 685             


Leu Ser Thr Ala Val Ser Ala 
    690                 695 


<210>  9643
<211>  691
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant

<400>  9643

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Ser Asp Ala Gly Lys Ala Ile Ser Phe 
        35                  40                  45              


Ala Thr Thr Leu Gly Val Asn Lys Cys His Val Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Lys Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Val Ala Val Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala 
            340                 345                 350         


Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile 
        515                 520                 525             


Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
        595                 600                 605             


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val 
    610                 615                 620                 


Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
625                 630                 635                 640 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
                645                 650                 655     


Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
            660                 665                 670         


Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala 
        675                 680                 685             


Val Ser Ala 
    690     


<210>  9644
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant

<400>  9644

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
        595                 600                 605             


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe 
625                 630                 635                 640 


Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln 
                645                 650                 655     


Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn 
            660                 665                 670         


Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe 
        675                 680                 685             


Leu Ser Thr Ala Val Ser Ala 
    690                 695 


<210>  9645
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant

<400>  9645

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
    610                 615                 620                 


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
                645                 650                 655     


Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
            660                 665                 670         


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685             


<210>  9646
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant

<400>  9646

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
    610                 615                 620                 


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
                645                 650                 655     


Met Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys 
            660                 665                 670         


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685             


<210>  9647
<211>  684
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant

<400>  9647

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn 
        35                  40                  45              


Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn 
            340                 345                 350         


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
        355                 360                 365             


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
    370                 375                 380                 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
385                 390                 395                 400 


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
                405                 410                 415     


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
            420                 425                 430         


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
        435                 440                 445             


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
    450                 455                 460                 


Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
465                 470                 475                 480 


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
                485                 490                 495     


Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
            500                 505                 510         


Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp 
        515                 520                 525             


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
    530                 535                 540                 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
545                 550                 555                 560 


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile 
                565                 570                 575     


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
            580                 585                 590         


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
        595                 600                 605             


Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly 
    610                 615                 620                 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
625                 630                 635                 640 


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly 
                645                 650                 655     


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly 
            660                 665                 670         


Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 


<210>  9648
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant

<400>  9648

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
    610                 615                 620                 


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
                645                 650                 655     


Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
            660                 665                 670         


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685             


<210>  9649
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant

<400>  9649

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        595                 600                 605             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    610                 615                 620                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
625                 630                 635                 640 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                645                 650                 655     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly 
            660                 665                 670         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        675                 680                 685             


Ser Thr Ala Val Ser Ala 
    690                 


<210>  9650
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant

<400>  9650

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Thr Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        595                 600                 605             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    610                 615                 620                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
625                 630                 635                 640 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                645                 650                 655     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys Asn Gly 
            660                 665                 670         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        675                 680                 685             


Ser Thr Ala Val Ser Ala 
    690                 


<210>  9651
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant

<400>  9651

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Ile Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala 
        35                  40                  45              


Thr Thr Leu Gly Val Asn Lys Cys His Val Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Lys Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Val 
145                 150                 155                 160 


Ala Val Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys 
            340                 345                 350         


Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro 
        515                 520                 525             


Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 
        595                 600                 605             


Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe 
    610                 615                 620                 


Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 
625                 630                 635                 640 


Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 
                645                 650                 655     


Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu 
            660                 665                 670         


Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val 
        675                 680                 685             


Ser Ala 
    690 


<210>  9652
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant

<400>  9652

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala 
        595                 600                 605             


Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val 
    610                 615                 620                 


Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly 
625                 630                 635                 640 


Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile 
                645                 650                 655     


Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly 
            660                 665                 670         


Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu 
        675                 680                 685             


Ser Thr Ala Val Ser Ala 
    690                 


<210>  9653
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant

<400>  9653

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr Thr Leu Lys Gly 
        595                 600                 605             


Ala Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Ile Gly Gly Val Phe Asn Ser Ile Gly Arg Ala Val His Gln Val Phe 
625                 630                 635                 640 


Gly Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser Trp Ile Thr Gln 
                645                 650                 655     


Gly Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val Asn Ala Arg Asp 
            660                 665                 670         


Arg Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly Val Leu Val Phe 
        675                 680                 685             


Leu Ala Thr Asn Val His Ala 
    690                 695 


<210>  9654
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant

<400>  9654

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Thr Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr Thr Leu Lys Gly 
        595                 600                 605             


Ala Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Ile Gly Gly Val Phe Asn Ser Ile Gly Arg Ala Val His Gln Val Phe 
625                 630                 635                 640 


Gly Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser Trp Ile Thr Gln 
                645                 650                 655     


Gly Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val Asn Ala Arg Asp 
            660                 665                 670         


Arg Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly Val Leu Val Phe 
        675                 680                 685             


Leu Ala Thr Asn Val His Ala 
    690                 695 


<210>  9655
<211>  691
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant

<400>  9655

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Ile Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Ser Asp Ala Gly Lys Ala Ile Ser Phe 
        35                  40                  45              


Ala Thr Thr Leu Gly Val Asn Lys Cys His Val Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Lys Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Val Ala Val Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala 
            340                 345                 350         


Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
        355                 360                 365             


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
    370                 375                 380                 


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
385                 390                 395                 400 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
                405                 410                 415     


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
            420                 425                 430         


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
        435                 440                 445             


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
    450                 455                 460                 


Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu 
465                 470                 475                 480 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
                485                 490                 495     


Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr 
            500                 505                 510         


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile 
        515                 520                 525             


Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
    530                 535                 540                 


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
545                 550                 555                 560 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
                565                 570                 575     


Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
            580                 585                 590         


Thr Leu Gly Lys Ala Phe Ser Thr Thr Leu Lys Gly Ala Gln Arg Leu 
        595                 600                 605             


Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Ile Gly Gly Val 
    610                 615                 620                 


Phe Asn Ser Ile Gly Arg Ala Val His Gln Val Phe Gly Gly Ala Phe 
625                 630                 635                 640 


Arg Thr Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu Met Gly 
                645                 650                 655     


Ala Leu Leu Leu Trp Met Gly Val Asn Ala Arg Asp Arg Ser Ile Ala 
            660                 665                 670         


Leu Ala Phe Leu Ala Thr Gly Gly Val Leu Val Phe Leu Ala Thr Asn 
        675                 680                 685             


Val His Ala 
    690     


<210>  9656
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant

<400>  9656

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Val Thr Arg Arg Gly Ser Ala 
            20                  25                  30          


Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe 
        35                  40                  45              


Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu 
    50                  55                  60                  


Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp 
65                  70                  75                  80  


Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser 
                85                  90                  95      


Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg 
            100                 105                 110         


Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu 
        115                 120                 125             


Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His 
    130                 135                 140                 


Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu 
145                 150                 155                 160 


Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys 
                165                 170                 175     


Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile 
            180                 185                 190         


Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly 
        195                 200                 205             


Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val 
    210                 215                 220                 


Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr 
225                 230                 235                 240 


Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile 
                245                 250                 255     


Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr 
            260                 265                 270         


Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val 
        275                 280                 285             


Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu 
    290                 295                 300                 


Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser 
305                 310                 315                 320 


Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly 
                325                 330                 335     


Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp 
            340                 345                 350         


Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu 
        355                 360                 365             


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
    370                 375                 380                 


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
385                 390                 395                 400 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
                405                 410                 415     


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
            420                 425                 430         


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
        435                 440                 445             


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
    450                 455                 460                 


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu 
465                 470                 475                 480 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
                485                 490                 495     


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
            500                 505                 510         


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
        515                 520                 525             


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
    530                 535                 540                 


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
545                 550                 555                 560 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
                565                 570                 575     


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
            580                 585                 590         


Arg Ser Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr Thr Leu Lys Gly 
        595                 600                 605             


Ala Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
    610                 615                 620                 


Ile Gly Gly Val Phe Asn Ser Ile Gly Arg Ala Val His Gln Val Phe 
625                 630                 635                 640 


Gly Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser Trp Ile Thr Gln 
                645                 650                 655     


Gly Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val Asn Ala Arg Asp 
            660                 665                 670         


Arg Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly Val Leu Val Phe 
        675                 680                 685             


Leu Ala Thr Asn Val His Ala 
    690                 695 


<210>  9657
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant

<400>  9657

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Leu Gly 
            580                 585                 590         


Lys Ala Phe Ser Thr Thr Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Ile Gly Gly Val Phe Asn Ser 
    610                 615                 620                 


Ile Gly Arg Ala Val His Gln Val Phe Gly Gly Ala Phe Arg Thr Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu Met Gly Ala Leu Leu 
                645                 650                 655     


Leu Trp Met Gly Val Asn Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe 
            660                 665                 670         


Leu Ala Thr Gly Gly Val Leu Val Phe Leu Ala Thr Asn Val His Ala 
        675                 680                 685             


<210>  9658
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant

<400>  9658

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Thr Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Leu Gly 
            580                 585                 590         


Lys Ala Phe Ser Thr Thr Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Ile Gly Gly Val Phe Asn Ser 
    610                 615                 620                 


Ile Gly Arg Ala Val His Gln Val Phe Gly Gly Ala Phe Arg Thr Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu Met Gly Ala Leu Leu 
                645                 650                 655     


Leu Trp Met Gly Val Asn Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe 
            660                 665                 670         


Leu Ala Thr Gly Gly Val Leu Val Phe Leu Ala Thr Asn Val His Ala 
        675                 680                 685             


<210>  9659
<211>  684
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant

<400>  9659

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ile Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala Thr Thr Leu Gly Val Asn 
        35                  40                  45              


Lys Cys His Val Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn 
            340                 345                 350         


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
        355                 360                 365             


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
    370                 375                 380                 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
385                 390                 395                 400 


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
                405                 410                 415     


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
            420                 425                 430         


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
        435                 440                 445             


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
    450                 455                 460                 


Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
465                 470                 475                 480 


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
                485                 490                 495     


Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
            500                 505                 510         


Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp 
        515                 520                 525             


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
    530                 535                 540                 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
545                 550                 555                 560 


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile 
                565                 570                 575     


Thr His His Trp His Arg Ser Gly Ser Thr Leu Gly Lys Ala Phe Ser 
            580                 585                 590         


Thr Thr Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala 
        595                 600                 605             


Trp Asp Phe Gly Ser Ile Gly Gly Val Phe Asn Ser Ile Gly Arg Ala 
    610                 615                 620                 


Val His Gln Val Phe Gly Gly Ala Phe Arg Thr Leu Phe Gly Gly Met 
625                 630                 635                 640 


Ser Trp Ile Thr Gln Gly Leu Met Gly Ala Leu Leu Leu Trp Met Gly 
                645                 650                 655     


Val Asn Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly 
            660                 665                 670         


Gly Val Leu Val Phe Leu Ala Thr Asn Val His Ala 
        675                 680                 


<210>  9660
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant

<400>  9660

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
        195                 200                 205             


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Leu Gly 
            580                 585                 590         


Lys Ala Phe Ser Thr Thr Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu 
        595                 600                 605             


Gly Asp Thr Ala Trp Asp Phe Gly Ser Ile Gly Gly Val Phe Asn Ser 
    610                 615                 620                 


Ile Gly Arg Ala Val His Gln Val Phe Gly Gly Ala Phe Arg Thr Leu 
625                 630                 635                 640 


Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu Met Gly Ala Leu Leu 
                645                 650                 655     


Leu Trp Met Gly Val Asn Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe 
            660                 665                 670         


Leu Ala Thr Gly Gly Val Leu Val Phe Leu Ala Thr Asn Val His Ala 
        675                 680                 685             


<210>  9661
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant

<400>  9661

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr Thr Leu Lys Gly Ala 
        595                 600                 605             


Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Ile 
    610                 615                 620                 


Gly Gly Val Phe Asn Ser Ile Gly Arg Ala Val His Gln Val Phe Gly 
625                 630                 635                 640 


Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly 
                645                 650                 655     


Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val Asn Ala Arg Asp Arg 
            660                 665                 670         


Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly Val Leu Val Phe Leu 
        675                 680                 685             


Ala Thr Asn Val His Ala 
    690                 


<210>  9662
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant

<400>  9662

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Thr Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr Thr Leu Lys Gly Ala 
        595                 600                 605             


Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Ile 
    610                 615                 620                 


Gly Gly Val Phe Asn Ser Ile Gly Arg Ala Val His Gln Val Phe Gly 
625                 630                 635                 640 


Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly 
                645                 650                 655     


Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val Asn Ala Arg Asp Arg 
            660                 665                 670         


Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly Val Leu Val Phe Leu 
        675                 680                 685             


Ala Thr Asn Val His Ala 
    690                 


<210>  9663
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant

<400>  9663

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Ile Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Ser Asp Ala Gly Lys Ala Ile Ser Phe Ala 
        35                  40                  45              


Thr Thr Leu Gly Val Asn Lys Cys His Val Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Lys Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Val 
145                 150                 155                 160 


Ala Val Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys 
            340                 345                 350         


Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 
        355                 360                 365             


Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 
    370                 375                 380                 


Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 
385                 390                 395                 400 


Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 
                405                 410                 415     


Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys 
            420                 425                 430         


Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 
        435                 440                 445             


Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 
    450                 455                 460                 


Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys 
465                 470                 475                 480 


Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 
                485                 490                 495     


Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val 
            500                 505                 510         


Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro 
        515                 520                 525             


Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 
    530                 535                 540                 


Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 
545                 550                 555                 560 


Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 
                565                 570                 575     


Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr 
            580                 585                 590         


Leu Gly Lys Ala Phe Ser Thr Thr Leu Lys Gly Ala Gln Arg Leu Ala 
        595                 600                 605             


Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Ile Gly Gly Val Phe 
    610                 615                 620                 


Asn Ser Ile Gly Arg Ala Val His Gln Val Phe Gly Gly Ala Phe Arg 
625                 630                 635                 640 


Thr Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu Met Gly Ala 
                645                 650                 655     


Leu Leu Leu Trp Met Gly Val Asn Ala Arg Asp Arg Ser Ile Ala Leu 
            660                 665                 670         


Ala Phe Leu Ala Thr Gly Gly Val Leu Val Phe Leu Ala Thr Asn Val 
        675                 680                 685             


His Ala 
    690 


<210>  9664
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant

<400>  9664

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Val Thr Arg Arg Gly Ser Ala Tyr 
            20                  25                  30          


Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro 
        35                  40                  45              


Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly 
    50                  55                  60                  


His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu 
65                  70                  75                  80  


Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr 
                85                  90                  95      


Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg 
            100                 105                 110         


Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln 
        115                 120                 125             


Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu 
    130                 135                 140                 


Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala 
145                 150                 155                 160 


Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val 
                165                 170                 175     


Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg 
            180                 185                 190         


Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly 
        195                 200                 205             


Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met 
    210                 215                 220                 


Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val 
225                 230                 235                 240 


Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser 
                245                 250                 255     


Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu 
            260                 265                 270         


Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp 
        275                 280                 285             


Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val 
    290                 295                 300                 


Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile 
305                 310                 315                 320 


Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser 
                325                 330                 335     


Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu 
            340                 345                 350         


Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala 
        355                 360                 365             


Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr 
    370                 375                 380                 


Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His 
385                 390                 395                 400 


Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His 
                405                 410                 415     


Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu 
            420                 425                 430         


Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu 
        435                 440                 445             


Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu 
    450                 455                 460                 


Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys 
465                 470                 475                 480 


Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser 
                485                 490                 495     


Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu 
            500                 505                 510         


His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro 
        515                 520                 525             


Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro 
    530                 535                 540                 


Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu 
545                 550                 555                 560 


Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr 
                565                 570                 575     


Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg 
            580                 585                 590         


Ser Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr Thr Leu Lys Gly Ala 
        595                 600                 605             


Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Ile 
    610                 615                 620                 


Gly Gly Val Phe Asn Ser Ile Gly Arg Ala Val His Gln Val Phe Gly 
625                 630                 635                 640 


Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly 
                645                 650                 655     


Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val Asn Ala Arg Asp Arg 
            660                 665                 670         


Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly Val Leu Val Phe Leu 
        675                 680                 685             


Ala Thr Asn Val His Ala 
    690                 


<210>  9665
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant

<400>  9665

Met Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
1               5                   10                  15      


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
            20                  25                  30          


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
        35                  40                  45              


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
    50                  55                  60                  


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
65                  70                  75                  80  


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
                85                  90                  95      


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
            100                 105                 110         


Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
        115                 120                 125             


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
    130                 135                 140                 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
145                 150                 155                 160 


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
                165                 170                 175     


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
            180                 185                 190         


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
        195                 200                 205             


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
    210                 215                 220                 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
225                 230                 235                 240 


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
                245                 250                 255     


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
            260                 265                 270         


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
        275                 280                 285             


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
    290                 295                 300                 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
305                 310                 315                 320 


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
                325                 330                 335     


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
            340                 345                 350         


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
        355                 360                 365             


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
    370                 375                 380                 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
385                 390                 395                 400 


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
                405                 410                 415     


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
            420                 425                 430         


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
        435                 440                 445             


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
    450                 455                 460                 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
465                 470                 475                 480 


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
                485                 490                 495     


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
            500                 505                 510         


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
        515                 520                 525             


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
    530                 535                 540                 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
545                 550                 555                 560 


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
                565                 570                 575     


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
            580                 585                 590         


Val Ser Ala 
        595 


<210>  9666
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant

<400>  9666

Met Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
1               5                   10                  15      


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
            20                  25                  30          


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
        35                  40                  45              


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
    50                  55                  60                  


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
65                  70                  75                  80  


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
                85                  90                  95      


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
            100                 105                 110         


Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
        115                 120                 125             


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
    130                 135                 140                 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
145                 150                 155                 160 


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
                165                 170                 175     


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
            180                 185                 190         


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
        195                 200                 205             


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
    210                 215                 220                 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
225                 230                 235                 240 


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
                245                 250                 255     


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
            260                 265                 270         


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
        275                 280                 285             


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
    290                 295                 300                 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
305                 310                 315                 320 


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
                325                 330                 335     


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
            340                 345                 350         


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
        355                 360                 365             


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
    370                 375                 380                 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
385                 390                 395                 400 


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
                405                 410                 415     


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
            420                 425                 430         


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
        435                 440                 445             


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
    450                 455                 460                 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
465                 470                 475                 480 


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
                485                 490                 495     


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
            500                 505                 510         


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
        515                 520                 525             


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
    530                 535                 540                 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
545                 550                 555                 560 


Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser Ile Ser 
                565                 570                 575     


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
            580                 585                 590         


Val Ser Ala 
        595 


<210>  9667
<211>  591
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant

<400>  9667

Met Thr Ser Ile Gly Ile Ile Gly Leu Leu Leu Thr Thr Ala Met Ala 
1               5                   10                  15      


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
            20                  25                  30          


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Lys Val 
        35                  40                  45              


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala Val Ala 
    50                  55                  60                  


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
65                  70                  75                  80  


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
                85                  90                  95      


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
            100                 105                 110         


Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
        115                 120                 125             


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
    130                 135                 140                 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
145                 150                 155                 160 


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
                165                 170                 175     


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
            180                 185                 190         


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
        195                 200                 205             


Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
    210                 215                 220                 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
225                 230                 235                 240 


Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val Glu Val 
                245                 250                 255     


Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser 
            260                 265                 270         


Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu 
        275                 280                 285             


Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp 
    290                 295                 300                 


Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr 
305                 310                 315                 320 


Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His 
                325                 330                 335     


Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val 
            340                 345                 350         


His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys 
        355                 360                 365             


Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys 
    370                 375                 380                 


Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr 
385                 390                 395                 400 


Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu 
                405                 410                 415     


Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met 
            420                 425                 430         


Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala 
        435                 440                 445             


Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu 
    450                 455                 460                 


Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp 
465                 470                 475                 480 


Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys 
                485                 490                 495     


Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly 
            500                 505                 510         


Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu 
        515                 520                 525             


Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe 
    530                 535                 540                 


Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val 
545                 550                 555                 560 


Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu 
                565                 570                 575     


Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser Ala 
            580                 585                 590     


<210>  9668
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant

<400>  9668

Met Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala Met Ala 
1               5                   10                  15      


Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
            20                  25                  30          


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
        35                  40                  45              


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
    50                  55                  60                  


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
65                  70                  75                  80  


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly 
                85                  90                  95      


Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val 
            100                 105                 110         


Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp 
        115                 120                 125             


Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met 
    130                 135                 140                 


Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala 
145                 150                 155                 160 


Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln 
                165                 170                 175     


Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp 
            180                 185                 190         


Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala 
        195                 200                 205             


Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu 
    210                 215                 220                 


Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser 
225                 230                 235                 240 


Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala 
                245                 250                 255     


Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly 
            260                 265                 270         


Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp 
        275                 280                 285             


Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val 
    290                 295                 300                 


His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala 
305                 310                 315                 320 


Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe 
                325                 330                 335     


Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln 
            340                 345                 350         


Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met 
        355                 360                 365             


Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu 
    370                 375                 380                 


Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr 
385                 390                 395                 400 


Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr 
                405                 410                 415     


Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val 
            420                 425                 430         


Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg 
        435                 440                 445             


Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys 
    450                 455                 460                 


Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
465                 470                 475                 480 


Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser 
                485                 490                 495     


Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met 
            500                 505                 510         


Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala 
        515                 520                 525             


Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe 
    530                 535                 540                 


Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly 
545                 550                 555                 560 


Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser 
                565                 570                 575     


Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala 
            580                 585                 590         


Val Ser Ala 
        595 


<210>  9669
<211>  604
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant

<400>  9669

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Ala Val Thr Leu Pro Ser His 
            20                  25                  30          


Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg 
        35                  40                  45              


Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn 
    50                  55                  60                  


Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser 
65                  70                  75                  80  


Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala 
                85                  90                  95      


Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val 
            100                 105                 110         


Glu Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly 
        115                 120                 125             


Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu 
    130                 135                 140                 


Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys 
145                 150                 155                 160 


Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr 
                165                 170                 175     


Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys 
            180                 185                 190         


Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe 
        195                 200                 205             


Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys 
    210                 215                 220                 


Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met 
225                 230                 235                 240 


Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr 
                245                 250                 255     


Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn 
            260                 265                 270         


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
        275                 280                 285             


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
    290                 295                 300                 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
305                 310                 315                 320 


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
                325                 330                 335     


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
            340                 345                 350         


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
        355                 360                 365             


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
    370                 375                 380                 


Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
385                 390                 395                 400 


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
                405                 410                 415     


Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
            420                 425                 430         


Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp 
        435                 440                 445             


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
    450                 455                 460                 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
465                 470                 475                 480 


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile 
                485                 490                 495     


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
            500                 505                 510         


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
        515                 520                 525             


Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly 
    530                 535                 540                 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
545                 550                 555                 560 


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly 
                565                 570                 575     


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly 
            580                 585                 590         


Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        595                 600                 


<210>  9670
<211>  604
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant

<400>  9670

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Ala Val Thr Leu Pro Ser His 
            20                  25                  30          


Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg 
        35                  40                  45              


Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn 
    50                  55                  60                  


Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser 
65                  70                  75                  80  


Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala 
                85                  90                  95      


Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val 
            100                 105                 110         


Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly 
        115                 120                 125             


Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu 
    130                 135                 140                 


Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys 
145                 150                 155                 160 


Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr 
                165                 170                 175     


Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys 
            180                 185                 190         


Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe 
        195                 200                 205             


Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys 
    210                 215                 220                 


Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met 
225                 230                 235                 240 


Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr 
                245                 250                 255     


Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn 
            260                 265                 270         


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
        275                 280                 285             


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
    290                 295                 300                 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
305                 310                 315                 320 


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
                325                 330                 335     


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
            340                 345                 350         


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
        355                 360                 365             


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
    370                 375                 380                 


Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
385                 390                 395                 400 


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
                405                 410                 415     


Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
            420                 425                 430         


Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp 
        435                 440                 445             


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
    450                 455                 460                 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
465                 470                 475                 480 


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile 
                485                 490                 495     


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
            500                 505                 510         


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
        515                 520                 525             


Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly 
    530                 535                 540                 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
545                 550                 555                 560 


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly 
                565                 570                 575     


Leu Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly 
            580                 585                 590         


Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        595                 600                 


<210>  9671
<211>  600
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant

<400>  9671

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Ala Val Thr Leu Pro Ser His 
            20                  25                  30          


Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg 
        35                  40                  45              


Glu Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe Arg Asn 
    50                  55                  60                  


Pro Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu Gly Ser 
65                  70                  75                  80  


Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala 
                85                  90                  95      


Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val 
            100                 105                 110         


Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly 
        115                 120                 125             


Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu 
    130                 135                 140                 


Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys 
145                 150                 155                 160 


Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr 
                165                 170                 175     


Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys 
            180                 185                 190         


Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe 
        195                 200                 205             


Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys 
    210                 215                 220                 


Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met 
225                 230                 235                 240 


Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr 
                245                 250                 255     


Asp Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 
            260                 265                 270         


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
        275                 280                 285             


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
    290                 295                 300                 


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
305                 310                 315                 320 


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
                325                 330                 335     


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
            340                 345                 350         


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
        355                 360                 365             


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 
    370                 375                 380                 


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
385                 390                 395                 400 


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu 
                405                 410                 415     


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
            420                 425                 430         


Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu 
        435                 440                 445             


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
    450                 455                 460                 


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
465                 470                 475                 480 


Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp 
                485                 490                 495     


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
            500                 505                 510         


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
        515                 520                 525             


Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
    530                 535                 540                 


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
545                 550                 555                 560 


Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys 
                565                 570                 575     


Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 
            580                 585                 590         


Phe Leu Ser Thr Ala Val Ser Ala 
        595                 600 


<210>  9672
<211>  604
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant

<400>  9672

Met Ala Ile Ser Gly Val Pro Val Leu Gly Phe Phe Ile Ile Ala Val 
1               5                   10                  15      


Leu Met Ser Ala Gln Glu Ser Trp Ala Ala Val Thr Leu Pro Ser His 
            20                  25                  30          


Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg 
        35                  40                  45              


Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn 
    50                  55                  60                  


Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser 
65                  70                  75                  80  


Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala 
                85                  90                  95      


Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val 
            100                 105                 110         


Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly 
        115                 120                 125             


Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu 
    130                 135                 140                 


Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys 
145                 150                 155                 160 


Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr 
                165                 170                 175     


Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys 
            180                 185                 190         


Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe 
        195                 200                 205             


Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys 
    210                 215                 220                 


Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met 
225                 230                 235                 240 


Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr 
                245                 250                 255     


Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn 
            260                 265                 270         


Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu 
        275                 280                 285             


Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu 
    290                 295                 300                 


Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp 
305                 310                 315                 320 


Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp 
                325                 330                 335     


Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg 
            340                 345                 350         


Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala 
        355                 360                 365             


Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu 
    370                 375                 380                 


Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu 
385                 390                 395                 400 


Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys 
                405                 410                 415     


Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr 
            420                 425                 430         


Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp 
        435                 440                 445             


Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val 
    450                 455                 460                 


Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro 
465                 470                 475                 480 


Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile 
                485                 490                 495     


Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu 
            500                 505                 510         


Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala 
        515                 520                 525             


Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly 
    530                 535                 540                 


Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met 
545                 550                 555                 560 


Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly 
                565                 570                 575     


Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly 
            580                 585                 590         


Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        595                 600                 


<210>  9673
<211>  597
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant

<400>  9673

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr 
            20                  25                  30          


Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile 
        35                  40                  45              


Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala 
    50                  55                  60                  


Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile 
65                  70                  75                  80  


Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys 
                85                  90                  95      


Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr 
            100                 105                 110         


Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala 
        115                 120                 125             


Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser 
    130                 135                 140                 


Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp 
145                 150                 155                 160 


Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp 
                165                 170                 175     


Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg 
            180                 185                 190         


Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr 
        195                 200                 205             


Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln 
    210                 215                 220                 


Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln 
225                 230                 235                 240 


His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn 
                245                 250                 255     


Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr 
            260                 265                 270         


Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly 
        275                 280                 285             


Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp 
    290                 295                 300                 


Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala 
305                 310                 315                 320 


Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val 
                325                 330                 335     


Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly 
            340                 345                 350         


Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala 
        355                 360                 365             


Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys 
    370                 375                 380                 


Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu 
385                 390                 395                 400 


Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His 
                405                 410                 415     


Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys 
            420                 425                 430         


Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val 
        435                 440                 445             


Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn 
    450                 455                 460                 


Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile 
465                 470                 475                 480 


Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser 
                485                 490                 495     


Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys 
            500                 505                 510         


Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 
        515                 520                 525             


Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala 
    530                 535                 540                 


Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu 
545                 550                 555                 560 


Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser 
                565                 570                 575     


Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser 
            580                 585                 590         


Thr Ala Val Ser Ala 
        595         


<210>  9674
<211>  597
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant

<400>  9674

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr 
            20                  25                  30          


Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile 
        35                  40                  45              


Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala 
    50                  55                  60                  


Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile 
65                  70                  75                  80  


Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys 
                85                  90                  95      


Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr 
            100                 105                 110         


Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala 
        115                 120                 125             


Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser 
    130                 135                 140                 


Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp 
145                 150                 155                 160 


Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp 
                165                 170                 175     


Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg 
            180                 185                 190         


Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr 
        195                 200                 205             


Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln 
    210                 215                 220                 


Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln 
225                 230                 235                 240 


His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn 
                245                 250                 255     


Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr 
            260                 265                 270         


Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly 
        275                 280                 285             


Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp 
    290                 295                 300                 


Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala 
305                 310                 315                 320 


Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val 
                325                 330                 335     


Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly 
            340                 345                 350         


Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala 
        355                 360                 365             


Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys 
    370                 375                 380                 


Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu 
385                 390                 395                 400 


Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His 
                405                 410                 415     


Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys 
            420                 425                 430         


Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val 
        435                 440                 445             


Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn 
    450                 455                 460                 


Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile 
465                 470                 475                 480 


Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser 
                485                 490                 495     


Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys 
            500                 505                 510         


Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 
        515                 520                 525             


Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala 
    530                 535                 540                 


Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu 
545                 550                 555                 560 


Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys Asn Gly Ser 
                565                 570                 575     


Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser 
            580                 585                 590         


Thr Ala Val Ser Ala 
        595         


<210>  9675
<211>  593
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant

<400>  9675

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr 
            20                  25                  30          


Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile 
        35                  40                  45              


Lys Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Val Ala 
    50                  55                  60                  


Val Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile 
65                  70                  75                  80  


Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys 
                85                  90                  95      


Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr 
            100                 105                 110         


Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala 
        115                 120                 125             


Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser 
    130                 135                 140                 


Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp 
145                 150                 155                 160 


Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp 
                165                 170                 175     


Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg 
            180                 185                 190         


Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr 
        195                 200                 205             


Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln 
    210                 215                 220                 


Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln 
225                 230                 235                 240 


His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg Ala Lys Val 
                245                 250                 255     


Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe 
            260                 265                 270         


Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser 
        275                 280                 285             


Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys 
    290                 295                 300                 


Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr 
305                 310                 315                 320 


Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp 
                325                 330                 335     


Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly 
            340                 345                 350         


Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly 
        355                 360                 365             


Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys Met 
    370                 375                 380                 


Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala 
385                 390                 395                 400 


Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val Thr 
                405                 410                 415     


Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Ile Pro Val 
            420                 425                 430         


Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile 
        435                 440                 445             


Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met 
    450                 455                 460                 


Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val 
465                 470                 475                 480 


Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile 
                485                 490                 495     


Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val 
            500                 505                 510         


Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe Asn 
        515                 520                 525             


Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser 
    530                 535                 540                 


Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu 
545                 550                 555                 560 


Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Thr 
                565                 570                 575     


Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val Ser 
            580                 585                 590         


Ala 
    


<210>  9676
<211>  597
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant

<400>  9676

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr 
            20                  25                  30          


Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile 
        35                  40                  45              


Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala 
    50                  55                  60                  


Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile 
65                  70                  75                  80  


Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys 
                85                  90                  95      


Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr 
            100                 105                 110         


Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr Val Met Ala 
        115                 120                 125             


Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser 
    130                 135                 140                 


Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp 
145                 150                 155                 160 


Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp 
                165                 170                 175     


Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg 
            180                 185                 190         


Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr 
        195                 200                 205             


Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln 
    210                 215                 220                 


Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln 
225                 230                 235                 240 


His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn 
                245                 250                 255     


Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr 
            260                 265                 270         


Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly 
        275                 280                 285             


Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp 
    290                 295                 300                 


Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala 
305                 310                 315                 320 


Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val 
                325                 330                 335     


Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly 
            340                 345                 350         


Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala 
        355                 360                 365             


Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys 
    370                 375                 380                 


Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu 
385                 390                 395                 400 


Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His 
                405                 410                 415     


Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys 
            420                 425                 430         


Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val 
        435                 440                 445             


Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn 
    450                 455                 460                 


Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile 
465                 470                 475                 480 


Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His Arg Ser 
                485                 490                 495     


Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys 
            500                 505                 510         


Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 
        515                 520                 525             


Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala 
    530                 535                 540                 


Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu 
545                 550                 555                 560 


Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser 
                565                 570                 575     


Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser 
            580                 585                 590         


Thr Ala Val Ser Ala 
        595         


<210>  9677
<211>  603
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant

<400>  9677

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Ala Val Thr Leu Pro Ser His Ser 
            20                  25                  30          


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
        35                  40                  45              


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
    50                  55                  60                  


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
65                  70                  75                  80  


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
                85                  90                  95      


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
            100                 105                 110         


Gly Met Ser Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly 
        115                 120                 125             


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
    130                 135                 140                 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
145                 150                 155                 160 


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
                165                 170                 175     


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
            180                 185                 190         


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
        195                 200                 205             


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
    210                 215                 220                 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
225                 230                 235                 240 


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
                245                 250                 255     


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
            260                 265                 270         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        275                 280                 285             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    290                 295                 300                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
305                 310                 315                 320 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                325                 330                 335     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            340                 345                 350         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        355                 360                 365             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
    370                 375                 380                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
385                 390                 395                 400 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
                405                 410                 415     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            420                 425                 430         


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
        435                 440                 445             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    450                 455                 460                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
465                 470                 475                 480 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
                485                 490                 495     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            500                 505                 510         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        515                 520                 525             


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
    530                 535                 540                 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
545                 550                 555                 560 


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
                565                 570                 575     


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
            580                 585                 590         


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        595                 600             


<210>  9678
<211>  603
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant

<400>  9678

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Ala Val Thr Leu Pro Ser His Ser 
            20                  25                  30          


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
        35                  40                  45              


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
    50                  55                  60                  


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
65                  70                  75                  80  


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
                85                  90                  95      


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
            100                 105                 110         


Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly 
        115                 120                 125             


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
    130                 135                 140                 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
145                 150                 155                 160 


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
                165                 170                 175     


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
            180                 185                 190         


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
        195                 200                 205             


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
    210                 215                 220                 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
225                 230                 235                 240 


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
                245                 250                 255     


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
            260                 265                 270         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        275                 280                 285             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    290                 295                 300                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
305                 310                 315                 320 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                325                 330                 335     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            340                 345                 350         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        355                 360                 365             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
    370                 375                 380                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
385                 390                 395                 400 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
                405                 410                 415     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            420                 425                 430         


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
        435                 440                 445             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    450                 455                 460                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
465                 470                 475                 480 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
                485                 490                 495     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            500                 505                 510         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        515                 520                 525             


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
    530                 535                 540                 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
545                 550                 555                 560 


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
                565                 570                 575     


Asn Ala Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
            580                 585                 590         


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        595                 600             


<210>  9679
<211>  599
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant

<400>  9679

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Ala Val Thr Leu Pro Ser His Ser 
            20                  25                  30          


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
        35                  40                  45              


Tyr Thr Lys His Leu Ile Lys Val Glu Asn Trp Ile Phe Arg Asn Pro 
    50                  55                  60                  


Gly Phe Ala Leu Val Ala Val Ala Ile Ala Trp Leu Leu Gly Ser Ser 
65                  70                  75                  80  


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
                85                  90                  95      


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
            100                 105                 110         


Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly 
        115                 120                 125             


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
    130                 135                 140                 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
145                 150                 155                 160 


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
                165                 170                 175     


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
            180                 185                 190         


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
        195                 200                 205             


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met 
    210                 215                 220                 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
225                 230                 235                 240 


Ser Val His Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp 
                245                 250                 255     


Glu Asp Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu 
            260                 265                 270         


Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg 
        275                 280                 285             


Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys 
    290                 295                 300                 


His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp 
305                 310                 315                 320 


His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala 
                325                 330                 335     


Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val 
            340                 345                 350         


Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu 
        355                 360                 365             


Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu 
    370                 375                 380                 


Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr 
385                 390                 395                 400 


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr 
                405                 410                 415     


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
            420                 425                 430         


Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr 
        435                 440                 445             


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
    450                 455                 460                 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
465                 470                 475                 480 


Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His 
                485                 490                 495     


Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly 
            500                 505                 510         


Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 
        515                 520                 525             


Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe 
    530                 535                 540                 


Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln 
545                 550                 555                 560 


Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn 
                565                 570                 575     


Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe 
            580                 585                 590         


Leu Ser Thr Ala Val Ser Ala 
        595                 


<210>  9680
<211>  603
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant

<400>  9680

Met Gly Lys Arg Ser Ala Gly Ser Ile Met Trp Leu Ala Ser Leu Ala 
1               5                   10                  15      


Val Val Ile Ala Cys Ala Gly Ala Ala Val Thr Leu Pro Ser His Ser 
            20                  25                  30          


Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu 
        35                  40                  45              


Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro 
    50                  55                  60                  


Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser 
65                  70                  75                  80  


Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro 
                85                  90                  95      


Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu 
            100                 105                 110         


Gly Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly 
        115                 120                 125             


Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu 
    130                 135                 140                 


Val Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr 
145                 150                 155                 160 


Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln 
                165                 170                 175     


Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys 
            180                 185                 190         


Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly 
        195                 200                 205             


Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met 
    210                 215                 220                 


Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu 
225                 230                 235                 240 


Ser Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly 
                245                 250                 255     


His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser 
            260                 265                 270         


Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp 
        275                 280                 285             


Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr 
    290                 295                 300                 


Met Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile 
305                 310                 315                 320 


Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn 
                325                 330                 335     


Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln 
            340                 345                 350         


Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu 
        355                 360                 365             


Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser 
    370                 375                 380                 


Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys 
385                 390                 395                 400 


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
                405                 410                 415     


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            420                 425                 430         


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
        435                 440                 445             


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    450                 455                 460                 


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
465                 470                 475                 480 


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
                485                 490                 495     


His His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala 
            500                 505                 510         


Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp 
        515                 520                 525             


Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile 
    530                 535                 540                 


His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser 
545                 550                 555                 560 


Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu 
                565                 570                 575     


Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly 
            580                 585                 590         


Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        595                 600             


<210>  9681
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS wild type

<400>  9681
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcu                     2085


<210>  9682
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS wild type

<400>  9682
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacac gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu      660

ugugucacug uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggagcc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa aguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggggu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacuggcugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacu     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaaucuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1980

acguugcuga ugugguuggg ucugaacgca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcu                     2085


<210>  9683
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS wild type

<400>  9683
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcuaucac uagacgcggg agugcauacu acauguacuu ggauaggagc      120

gaugccggga aggccauuuc guuugcuacc acauugggag ugaacaagug ccacguacag      180

aucauggacc ucgggcacau gugugacgcc accaugaguu augagugccc uaugcuggau      240

gagggagugg aaccagauga ugucgauugc uggugcaaca cgacaucaac uuggguugug      300

uacggaaccu gucaucacaa aaaaggugag gcacggcgau cuagaagagc cgugacgcuc      360

ccuucucacu cuacaaggaa guugcaaacg cggucgcaga ccugguuaga aucaagagaa      420

uacacgaagc acuugaucaa gguugaaaac uggauauuca ggaaccccgg guuugcgcua      480

guggccguug ccauugccug gcuuuuggga agcucgacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac aguaucaggu gcauuggagu cagcaauaga      600

gacuucgugg agggcauguc aggugggacc uggguugaug uugucuugga acauggaggc      660

ugcguuaccg ugauggcaca ggacaagcca acagucgaca uagaguuggu cacgacgacg      720

guuaguaaca uggccgaggu aagauccuau ugcuacgagg caucgauauc ggacauggcu      780

ucggacaguc guugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacauu aguggacaga gguuggggaa acgguugugg acuuuuuggc      900

aaagggagcu uggugacaug ugccaaguuu acguguucua agaagaugac cgggaagagc      960

auucaaccgg aaaaucugga guaucggaua augcuaucag ugcauggcuc ccagcauagc     1020

gggaugauug gauaugaaac ugacgaagau agagcgaaag ucgagguuac gccuaauuca     1080

ccaagagcgg aagcaaccuu gggaggcuuu ggaaguuuag gacuugacug ugaaccaagg     1140

acaggccuug acuuuucaga ucuguauuac cugaccauga acaauaagca uugguuggug     1200

cacaaagagu gguuucauga caucccauug ccuuggcaug cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga ggcauuggua gaauuuaagg augcccacgc caagaggcaa     1320

accgucgucg uucuggggag ccaggaagga gccguucaca cggcucucgc uggagcucua     1380

gaggcugaga uggauggugc aaagggaagg cuguucucug gccauuugaa augccgccua     1440

aaaauggaca agcucagauu gaagggcgug ucauauuccu ugugcacugc ggcauucaca     1500

uucaccaagg ucccagcuga aacacugcau ggaacaguca caguggaggu gcaguaugca     1560

gggacagaug gacccugcaa gaucccaguc cagauggcgg uggacaugca gacccugacc     1620

ccaguuggaa ggcugauaac cgccaacccc gugauuacug aaagcacuga gaacucaaag     1680

augauguugg agcuugaccc accauuuggg gauucuuaca uugucauagg aguuggggac     1740

aagaaaauca cccaccacug gcauaggagu gguagcacca ucggaaaggc auuugaggcc     1800

acugugagag gcgccaagag aauggcaguc cugggggaua cagccuggga cuucggauca     1860

gucgggggug uguucaacuc acuggguaag ggcauucacc agauuuuugg agcagccuuc     1920

aaaucacugu uuggaggaau guccugguuc ucacagaucc ucauaggcac gcugcuagug     1980

ugguuagguu ugaacacaaa gaauggaucu aucucccuca caugcuuggc ccugggggga     2040

gugaugaucu uccucuccac ggcuguuucu gcu                                  2073


<210>  9684
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS wild type

<400>  9684
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggagcc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uccacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1800

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1860

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1920

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ccucauugga     1980

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     2040

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcu                     2085


<210>  9685
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS wild type

<400>  9685
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1800

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1860

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1920

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     1980

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2040

uucuuaucca cagccgucuc ugcu                                            2064


<210>  9686
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS wild type

<400>  9686
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1800

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1860

gcucucaacu cauugggcaa gggcauccau caaaucuuug gagcagcuuu caaaucauug     1920

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     1980

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2040

uucuuaucca cagccgucuc ugcu                                            2064


<210>  9687
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS wild type

<400>  9687
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcaucacu       60

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      120

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      180

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      240

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      300

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      360

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      420

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      480

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      600

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      660

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      720

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      840

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      900

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      960

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1020

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1080

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1140

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1200

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1260

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1320

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1380

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug     1440

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1500

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1560

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1620

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1680

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1740

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1800

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1860

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1920

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     1980

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     2040

gcuguuucug cu                                                         2052


<210>  9688
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS wild type

<400>  9688
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1800

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1860

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1920

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     1980

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     2040

uucuuaucca cagccgucuc ugcu                                            2064


<210>  9689
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS wild type

<400>  9689
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cu                        2082


<210>  9690
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS wild type

<400>  9690
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacacgug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauguug ucuuggaaca uggagguugu      660

gucacuguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagccugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaagu ugagauaacg     1080

cccaauucac caagagccga agccacccug gggggguuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

uggcugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguacugca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aaucuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauucu cauuggaacg     1980

uugcugaugu gguugggucu gaacgcaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cu                        2082


<210>  9691
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS wild type

<400>  9691
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccaucacuag acgcgggagu gcauacuaca uguacuugga uaggagcgau      120

gccgggaagg ccauuucguu ugcuaccaca uugggaguga acaagugcca cguacagauc      180

auggaccucg ggcacaugug ugacgccacc augaguuaug agugcccuau gcuggaugag      240

ggaguggaac cagaugaugu cgauugcugg ugcaacacga caucaacuug gguuguguac      300

ggaaccuguc aucacaaaaa aggugaggca cggcgaucua gaagagccgu gacgcucccu      360

ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu gguuagaauc aagagaauac      420

acgaagcacu ugaucaaggu ugaaaacugg auauucagga accccggguu ugcgcuagug      480

gccguugcca uugccuggcu uuugggaagc ucgacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagu aucaggugca uuggagucag caauagagac      600

uucguggagg gcaugucagg ugggaccugg guugauguug ucuuggaaca uggaggcugc      660

guuaccguga uggcacagga caagccaaca gucgacauag aguuggucac gacgacgguu      720

aguaacaugg ccgagguaag auccuauugc uacgaggcau cgauaucgga cauggcuucg      780

gacagucguu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacauuagu ggacagaggu uggggaaacg guuguggacu uuuuggcaaa      900

gggagcuugg ugacaugugc caaguuuacg uguucuaaga agaugaccgg gaagagcauu      960

caaccggaaa aucuggagua ucggauaaug cuaucagugc auggcuccca gcauagcggg     1020

augauuggau augaaacuga cgaagauaga gcgaaagucg agguuacgcc uaauucacca     1080

agagcggaag caaccuuggg aggcuuugga aguuuaggac uugacuguga accaaggaca     1140

ggccuugacu uuucagaucu guauuaccug accaugaaca auaagcauug guuggugcac     1200

aaagaguggu uucaugacau cccauugccu uggcaugcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaggc auugguagaa uuuaaggaug cccacgccaa gaggcaaacc     1320

gucgucguuc uggggagcca ggaaggagcc guucacacgg cucucgcugg agcucuagag     1380

gcugagaugg auggugcaaa gggaaggcug uucucuggcc auuugaaaug ccgccuaaaa     1440

auggacaagc ucagauugaa gggcguguca uauuccuugu gcacugcggc auucacauuc     1500

accaaggucc cagcugaaac acugcaugga acagucacag uggaggugca guaugcaggg     1560

acagauggac ccugcaagau cccaguccag auggcggugg acaugcagac ccugacccca     1620

guuggaaggc ugauaaccgc caaccccgug auuacugaaa gcacugagaa cucaaagaug     1680

auguuggagc uugacccacc auuuggggau ucuuacauug ucauaggagu uggggacaag     1740

aaaaucaccc accacuggca uaggaguggu agcaccaucg gaaaggcauu ugaggccacu     1800

gugagaggcg ccaagagaau ggcaguccug ggggauacag ccugggacuu cggaucaguc     1860

gggggugugu ucaacucacu ggguaagggc auucaccaga uuuuuggagc agccuucaaa     1920

ucacuguuug gaggaauguc cugguucuca cagauccuca uaggcacgcu gcuagugugg     1980

uuagguuuga acacaaagaa uggaucuauc ucccucacau gcuuggcccu ggggggagug     2040

augaucuucc ucuccacggc uguuucugcu                                      2070


<210>  9692
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS wild type

<400>  9692
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauguug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagccugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguuggucc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcaccau uggaaaagca     1800

uuugaagcca cugugagagg ugccaagaga auggcagucu ugggagacac agccugggac     1860

uuuggaucag uuggaggcgc ucucaacuca uugggcaagg gcauccauca aauuuuugga     1920

gcagcuuuca aaucauuguu uggaggaaug uccugguucu cacaaauccu cauuggaacg     1980

uugcugaugu gguugggucu gaacacaaag aauggaucua uuucccuuau gugcuuggcc     2040

uuagggggag uguugaucuu cuuauccaca gccgucucug cu                        2082


<210>  9693
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS wild type

<400>  9693
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac gcugggcaag     1800

gccuuuucaa caacuuugaa gggagcucaa agacuggcag cguugggcga cacagccugg     1860

gacuuuggcu cuauuggagg ggucuucaac uccauaggaa gagccguuca ccaaguguuu     1920

gguggugccu ucagaacacu cuuuggggga augucuugga ucacacaagg gcuaaugggu     1980

gcccuacugc ucuggauggg cgucaacgca cgagaccgau caauugcuuu ggccuucuua     2040

gccacaggag gugugcucgu guucuuagcg accaaugugc acgcc                     2085


<210>  9694
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS wild type

<400>  9694
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacac gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu      660

ugugucacug uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggagcc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa aguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggggu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacuggcugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacu     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac gcugggcaag     1800

gccuuuucaa caacuuugaa gggagcucaa agacuggcag cguugggcga cacagccugg     1860

gacuuuggcu cuauuggagg ggucuucaac uccauaggaa gagccguuca ccaaguguuu     1920

gguggugccu ucagaacacu cuuuggggga augucuugga ucacacaagg gcuaaugggu     1980

gcccuacugc ucuggauggg cgucaacgca cgagaccgau caauugcuuu ggccuucuua     2040

gccacaggag gugugcucgu guucuuagcg accaaugugc acgcc                     2085


<210>  9695
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS wild type

<400>  9695
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcuaucac uagacgcggg agugcauacu acauguacuu ggauaggagc      120

gaugccggga aggccauuuc guuugcuacc acauugggag ugaacaagug ccacguacag      180

aucauggacc ucgggcacau gugugacgcc accaugaguu augagugccc uaugcuggau      240

gagggagugg aaccagauga ugucgauugc uggugcaaca cgacaucaac uuggguugug      300

uacggaaccu gucaucacaa aaaaggugag gcacggcgau cuagaagagc cgugacgcuc      360

ccuucucacu cuacaaggaa guugcaaacg cggucgcaga ccugguuaga aucaagagaa      420

uacacgaagc acuugaucaa gguugaaaac uggauauuca ggaaccccgg guuugcgcua      480

guggccguug ccauugccug gcuuuuggga agcucgacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac aguaucaggu gcauuggagu cagcaauaga      600

gacuucgugg agggcauguc aggugggacc uggguugaug uugucuugga acauggaggc      660

ugcguuaccg ugauggcaca ggacaagcca acagucgaca uagaguuggu cacgacgacg      720

guuaguaaca uggccgaggu aagauccuau ugcuacgagg caucgauauc ggacauggcu      780

ucggacaguc guugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacauu aguggacaga gguuggggaa acgguugugg acuuuuuggc      900

aaagggagcu uggugacaug ugccaaguuu acguguucua agaagaugac cgggaagagc      960

auucaaccgg aaaaucugga guaucggaua augcuaucag ugcauggcuc ccagcauagc     1020

gggaugauug gauaugaaac ugacgaagau agagcgaaag ucgagguuac gccuaauuca     1080

ccaagagcgg aagcaaccuu gggaggcuuu ggaaguuuag gacuugacug ugaaccaagg     1140

acaggccuug acuuuucaga ucuguauuac cugaccauga acaauaagca uugguuggug     1200

cacaaagagu gguuucauga caucccauug ccuuggcaug cuggggcaga caccggaacu     1260

ccacacugga acaacaaaga ggcauuggua gaauuuaagg augcccacgc caagaggcaa     1320

accgucgucg uucuggggag ccaggaagga gccguucaca cggcucucgc uggagcucua     1380

gaggcugaga uggauggugc aaagggaagg cuguucucug gccauuugaa augccgccua     1440

aaaauggaca agcucagauu gaagggcgug ucauauuccu ugugcacugc ggcauucaca     1500

uucaccaagg ucccagcuga aacacugcau ggaacaguca caguggaggu gcaguaugca     1560

gggacagaug gacccugcaa gaucccaguc cagauggcgg uggacaugca gacccugacc     1620

ccaguuggaa ggcugauaac cgccaacccc gugauuacug aaagcacuga gaacucaaag     1680

augauguugg agcuugaccc accauuuggg gauucuuaca uugucauagg aguuggggac     1740

aagaaaauca cccaccacug gcauaggagu ggcagcacgc ugggcaaggc cuuuucaaca     1800

acuuugaagg gagcucaaag acuggcagcg uugggcgaca cagccuggga cuuuggcucu     1860

auuggagggg ucuucaacuc cauaggaaga gccguucacc aaguguuugg uggugccuuc     1920

agaacacucu uugggggaau gucuuggauc acacaagggc uaaugggugc ccuacugcuc     1980

uggaugggcg ucaacgcacg agaccgauca auugcuuugg ccuucuuagc cacaggaggu     2040

gugcucgugu ucuuagcgac caaugugcac gcc                                  2073


<210>  9696
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS wild type

<400>  9696
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugucac uagacguggg agugcauacu auauguacuu ggacagaaac      120

gaugcugggg aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag      180

aucauggauc uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau      240

gagggggugg aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug      300

uacggaaccu gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc      360

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      420

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      480

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      540

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      600

gacuuugugg aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu      660

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      720

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      780

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      840

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      900

aaagggagcc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      960

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu     1020

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua     1080

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau     1140

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag     1200

cacugguugg uccacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1320

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1380

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1440

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1500

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1560

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1620

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1680

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1740

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac gcugggcaag     1800

gccuuuucaa caacuuugaa gggagcucaa agacuggcag cguugggcga cacagccugg     1860

gacuuuggcu cuauuggagg ggucuucaac uccauaggaa gagccguuca ccaaguguuu     1920

gguggugccu ucagaacacu cuuuggggga augucuugga ucacacaagg gcuaaugggu     1980

gcccuacugc ucuggauggg cgucaacgca cgagaccgau caauugcuuu ggccuucuua     2040

gccacaggag gugugcucgu guucuuagcg accaaugugc acgcc                     2085


<210>  9697
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS wild type

<400>  9697
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacg cugggcaagg ccuuuucaac aacuuugaag     1800

ggagcucaaa gacuggcagc guugggcgac acagccuggg acuuuggcuc uauuggaggg     1860

gucuucaacu ccauaggaag agccguucac caaguguuug guggugccuu cagaacacuc     1920

uuugggggaa ugucuuggau cacacaaggg cuaaugggug cccuacugcu cuggaugggc     1980

gucaacgcac gagaccgauc aauugcuuug gccuucuuag ccacaggagg ugugcucgug     2040

uucuuagcga ccaaugugca cgcc                                            2064


<210>  9698
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS wild type

<400>  9698
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacacg      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacg cugggcaagg ccuuuucaac aacuuugaag     1800

ggagcucaaa gacuggcagc guugggcgac acagccuggg acuuuggcuc uauuggaggg     1860

gucuucaacu ccauaggaag agccguucac caaguguuug guggugccuu cagaacacuc     1920

uuugggggaa ugucuuggau cacacaaggg cuaaugggug cccuacugcu cuggaugggc     1980

gucaacgcac gagaccgauc aauugcuuug gccuucuuag ccacaggagg ugugcucgug     2040

uucuuagcga ccaaugugca cgcc                                            2064


<210>  9699
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS wild type

<400>  9699
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcaucacu       60

agacgcggga gugcauacua cauguacuug gauaggagcg augccgggaa ggccauuucg      120

uuugcuacca cauugggagu gaacaagugc cacguacaga ucauggaccu cgggcacaug      180

ugugacgcca ccaugaguua ugagugcccu augcuggaug agggagugga accagaugau      240

gucgauugcu ggugcaacac gacaucaacu uggguugugu acggaaccug ucaucacaaa      300

aaaggugagg cacggcgauc uagaagagcc gugacgcucc cuucucacuc uacaaggaag      360

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      420

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      480

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      600

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      660

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      720

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      840

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      900

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      960

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu     1020

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug     1080

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau     1140

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac     1200

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1260

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1320

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1380

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug     1440

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1500

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1560

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1620

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1680

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1740

cauaggagug gcagcacgcu gggcaaggcc uuuucaacaa cuuugaaggg agcucaaaga     1800

cuggcagcgu ugggcgacac agccugggac uuuggcucua uuggaggggu cuucaacucc     1860

auaggaagag ccguucacca aguguuuggu ggugccuuca gaacacucuu ugggggaaug     1920

ucuuggauca cacaagggcu aaugggugcc cuacugcucu ggaugggcgu caacgcacga     1980

gaccgaucaa uugcuuuggc cuucuuagcc acaggaggug ugcucguguu cuuagcgacc     2040

aaugugcacg cc                                                         2052


<210>  9700
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS wild type

<400>  9700
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacu       60

agacguggga gugcauacua uauguacuug gacagaaacg augcugggga ggccauaucu      120

uuuccaacca cauuggggau gaauaagugu uauauacaga ucauggaucu uggacacaug      180

ugugaugcca ccaugagcua ugaaugcccu augcuggaug agggggugga accagaugac      240

gucgauuguu ggugcaacac gacgucaacu uggguugugu acggaaccug ccaucacaaa      300

aaaggugaag cacggagauc uagaagagcu gugacgcucc ccucccauuc cacuaggaag      360

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      420

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      480

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      540

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      600

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      660

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      720

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      780

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      840

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      900

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      960

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca     1020

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc     1080

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu     1140

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag     1200

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1260

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1320

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1380

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1440

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1500

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1560

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1620

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1680

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1740

acccaccacu ggcacaggag uggcagcacg cugggcaagg ccuuuucaac aacuuugaag     1800

ggagcucaaa gacuggcagc guugggcgac acagccuggg acuuuggcuc uauuggaggg     1860

gucuucaacu ccauaggaag agccguucac caaguguuug guggugccuu cagaacacuc     1920

uuugggggaa ugucuuggau cacacaaggg cuaaugggug cccuacugcu cuggaugggc     1980

gucaacgcac gagaccgauc aauugcuuug gccuucuuag ccacaggagg ugugcucgug     2040

uucuuagcga ccaaugugca cgcc                                            2064


<210>  9701
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS wild type

<400>  9701
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauauug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagucugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcacgcu gggcaaggcc     1800

uuuucaacaa cuuugaaggg agcucaaaga cuggcagcgu ugggcgacac agccugggac     1860

uuuggcucua uuggaggggu cuucaacucc auaggaagag ccguucacca aguguuuggu     1920

ggugccuuca gaacacucuu ugggggaaug ucuuggauca cacaagggcu aaugggugcc     1980

cuacugcucu ggaugggcgu caacgcacga gaccgaucaa uugcuuuggc cuucuuagcc     2040

acaggaggug ugcucguguu cuuagcgacc aaugugcacg cc                        2082


<210>  9702
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS wild type

<400>  9702
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacacgug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauguug ucuuggaaca uggagguugu      660

gucacuguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagccugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaagu ugagauaacg     1080

cccaauucac caagagccga agccacccug gggggguuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

uggcugguuc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguacugca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcacgcu gggcaaggcc     1800

uuuucaacaa cuuugaaggg agcucaaaga cuggcagcgu ugggcgacac agccugggac     1860

uuuggcucua uuggaggggu cuucaacucc auaggaagag ccguucacca aguguuuggu     1920

ggugccuuca gaacacucuu ugggggaaug ucuuggauca cacaagggcu aaugggugcc     1980

cuacugcucu ggaugggcgu caacgcacga gaccgaucaa uugcuuuggc cuucuuagcc     2040

acaggaggug ugcucguguu cuuagcgacc aaugugcacg cc                        2082


<210>  9703
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS wild type

<400>  9703
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccaucacuag acgcgggagu gcauacuaca uguacuugga uaggagcgau      120

gccgggaagg ccauuucguu ugcuaccaca uugggaguga acaagugcca cguacagauc      180

auggaccucg ggcacaugug ugacgccacc augaguuaug agugcccuau gcuggaugag      240

ggaguggaac cagaugaugu cgauugcugg ugcaacacga caucaacuug gguuguguac      300

ggaaccuguc aucacaaaaa aggugaggca cggcgaucua gaagagccgu gacgcucccu      360

ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu gguuagaauc aagagaauac      420

acgaagcacu ugaucaaggu ugaaaacugg auauucagga accccggguu ugcgcuagug      480

gccguugcca uugccuggcu uuugggaagc ucgacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagu aucaggugca uuggagucag caauagagac      600

uucguggagg gcaugucagg ugggaccugg guugauguug ucuuggaaca uggaggcugc      660

guuaccguga uggcacagga caagccaaca gucgacauag aguuggucac gacgacgguu      720

aguaacaugg ccgagguaag auccuauugc uacgaggcau cgauaucgga cauggcuucg      780

gacagucguu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacauuagu ggacagaggu uggggaaacg guuguggacu uuuuggcaaa      900

gggagcuugg ugacaugugc caaguuuacg uguucuaaga agaugaccgg gaagagcauu      960

caaccggaaa aucuggagua ucggauaaug cuaucagugc auggcuccca gcauagcggg     1020

augauuggau augaaacuga cgaagauaga gcgaaagucg agguuacgcc uaauucacca     1080

agagcggaag caaccuuggg aggcuuugga aguuuaggac uugacuguga accaaggaca     1140

ggccuugacu uuucagaucu guauuaccug accaugaaca auaagcauug guuggugcac     1200

aaagaguggu uucaugacau cccauugccu uggcaugcug gggcagacac cggaacucca     1260

cacuggaaca acaaagaggc auugguagaa uuuaaggaug cccacgccaa gaggcaaacc     1320

gucgucguuc uggggagcca ggaaggagcc guucacacgg cucucgcugg agcucuagag     1380

gcugagaugg auggugcaaa gggaaggcug uucucuggcc auuugaaaug ccgccuaaaa     1440

auggacaagc ucagauugaa gggcguguca uauuccuugu gcacugcggc auucacauuc     1500

accaaggucc cagcugaaac acugcaugga acagucacag uggaggugca guaugcaggg     1560

acagauggac ccugcaagau cccaguccag auggcggugg acaugcagac ccugacccca     1620

guuggaaggc ugauaaccgc caaccccgug auuacugaaa gcacugagaa cucaaagaug     1680

auguuggagc uugacccacc auuuggggau ucuuacauug ucauaggagu uggggacaag     1740

aaaaucaccc accacuggca uaggaguggc agcacgcugg gcaaggccuu uucaacaacu     1800

uugaagggag cucaaagacu ggcagcguug ggcgacacag ccugggacuu uggcucuauu     1860

ggaggggucu ucaacuccau aggaagagcc guucaccaag uguuuggugg ugccuucaga     1920

acacucuuug ggggaauguc uuggaucaca caagggcuaa ugggugcccu acugcucugg     1980

augggcguca acgcacgaga ccgaucaauu gcuuuggccu ucuuagccac aggaggugug     2040

cucguguucu uagcgaccaa ugugcacgcc                                      2070


<210>  9704
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS wild type

<400>  9704
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgucacuag acgugggagu gcauacuaua uguacuugga cagaaacgau      120

gcuggggagg ccauaucuuu uccaaccaca uuggggauga auaaguguua uauacagauc      180

auggaucuug gacacaugug ugaugccacc augagcuaug aaugcccuau gcuggaugag      240

gggguggaac cagaugacgu cgauuguugg ugcaacacga cgucaacuug gguuguguac      300

ggaaccugcc aucacaaaaa aggugaagca cggagaucua gaagagcugu gacgcucccc      360

ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu gguuggaauc aagagaauac      420

acaaagcacu ugauuagagu cgaaaauugg auauucagga acccuggcuu cgcguuagca      480

gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc aaaaagucau auacuugguc      540

augauacugc ugauugcccc ggcauacagc aucaggugca uaggagucag caauagggac      600

uuuguggaag guaugucagg ugggacuugg guugauguug ucuuggaaca uggagguugu      660

gucaccguaa uggcacagga caaaccgacu gucgacauag agcugguuac aacaacaguc      720

agcaacaugg cggagguaag auccuacugc uaugaggcau caauaucaga cauggcuucg      780

gacagccgcu gcccaacaca aggugaagcc uaccuugaca agcaaucaga cacucaauau      840

gucugcaaaa gaacguuagu ggacagaggc uggggaaaug gauguggacu uuuuggcaaa      900

gggagccugg ugacaugcgc uaaguuugca ugcuccaaga aaaugaccgg gaagagcauc      960

cagccagaga aucuggagua ccggauaaug cugucaguuc auggcuccca gcacaguggg     1020

augaucguua augacacagg acaugaaacu gaugagaaua gagcgaaggu ugagauaacg     1080

cccaauucac caagagccga agccacccug ggggguuuug gaagccuagg acuugauugu     1140

gaaccgagga caggccuuga cuuuucagau uuguauuacu ugacuaugaa uaacaagcac     1200

ugguuggucc acaaggagug guuccacgac auuccauuac cuuggcacgc uggggcagac     1260

accggaacuc cacacuggaa caacaaagaa gcacugguag aguucaagga cgcacaugcc     1320

aaaaggcaaa cugucguggu ucuagggagu caagaaggag caguucacac ggcccuugcu     1380

ggagcucugg aggcugagau ggauggugca aagggaaggc uguccucugg ccacuugaaa     1440

ugucgccuga aaauggauaa acuuagauug aagggcgugu cauacuccuu guguaccgca     1500

gcguucacau ucaccaagau cccggcugaa acacugcacg ggacagucac aguggaggua     1560

caguacgcag ggacagaugg accuugcaag guuccagcuc agauggcggu ggacaugcaa     1620

acucugaccc caguugggag guugauaacc gcuaaccccg uaaucacuga aagcacugag     1680

aacucuaaga ugaugcugga acuugaucca ccauuugggg acucuuacau ugucauagga     1740

gucggggaga agaagaucac ccaccacugg cacaggagug gcagcacgcu gggcaaggcc     1800

uuuucaacaa cuuugaaggg agcucaaaga cuggcagcgu ugggcgacac agccugggac     1860

uuuggcucua uuggaggggu cuucaacucc auaggaagag ccguucacca aguguuuggu     1920

ggugccuuca gaacacucuu ugggggaaug ucuuggauca cacaagggcu aaugggugcc     1980

cuacugcucu ggaugggcgu caacgcacga gaccgaucaa uugcuuuggc cuucuuagcc     2040

acaggaggug ugcucguguu cuuagcgacc aaugugcacg cc                        2082


<210>  9705
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS wild type

<400>  9705
augaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ugugacgcuc       60

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      120

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      180

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      240

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      300

gacuuugugg aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu      360

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      420

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      480

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      540

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      600

aaagggaguc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      660

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu      720

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua      780

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau      840

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag      900

cacugguugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca      960

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1020

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1080

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1140

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1200

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1260

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1320

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1380

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1440

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1500

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1560

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1620

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1680

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     1740

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcu                     1785


<210>  9706
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS wild type

<400>  9706
augacgagug ucggaauugu uggccuccug cugaccacag cuauggcagc ugugacgcuc       60

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      120

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      180

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      240

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      300

gacuuugugg aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu      360

ugugucacug uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      420

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      480

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      540

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      600

aaagggagcc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      660

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu      720

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa aguugagaua      780

acgcccaauu caccaagagc cgaagccacc cugggggggu uuggaagccu aggacuugau      840

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag      900

cacuggcugg uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca      960

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1020

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1080

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1140

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacu     1200

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1260

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1320

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1380

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1440

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1500

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1560

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaaucuuu     1620

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga     1680

acguugcuga ugugguuggg ucugaacgca aagaauggau cuauuucccu uaugugcuug     1740

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcu                     1785


<210>  9707
<211>  1773
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS wild type

<400>  9707
augaccagca ucggaaucau uggccuccug cugacuacag ccauggcagc cgugacgcuc       60

ccuucucacu cuacaaggaa guugcaaacg cggucgcaga ccugguuaga aucaagagaa      120

uacacgaagc acuugaucaa gguugaaaac uggauauuca ggaaccccgg guuugcgcua      180

guggccguug ccauugccug gcuuuuggga agcucgacga gccaaaaagu cauauacuug      240

gucaugauac ugcugauugc cccggcauac aguaucaggu gcauuggagu cagcaauaga      300

gacuucgugg agggcauguc aggugggacc uggguugaug uugucuugga acauggaggc      360

ugcguuaccg ugauggcaca ggacaagcca acagucgaca uagaguuggu cacgacgacg      420

guuaguaaca uggccgaggu aagauccuau ugcuacgagg caucgauauc ggacauggcu      480

ucggacaguc guugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      540

uaugucugca aaagaacauu aguggacaga gguuggggaa acgguugugg acuuuuuggc      600

aaagggagcu uggugacaug ugccaaguuu acguguucua agaagaugac cgggaagagc      660

auucaaccgg aaaaucugga guaucggaua augcuaucag ugcauggcuc ccagcauagc      720

gggaugauug gauaugaaac ugacgaagau agagcgaaag ucgagguuac gccuaauuca      780

ccaagagcgg aagcaaccuu gggaggcuuu ggaaguuuag gacuugacug ugaaccaagg      840

acaggccuug acuuuucaga ucuguauuac cugaccauga acaauaagca uugguuggug      900

cacaaagagu gguuucauga caucccauug ccuuggcaug cuggggcaga caccggaacu      960

ccacacugga acaacaaaga ggcauuggua gaauuuaagg augcccacgc caagaggcaa     1020

accgucgucg uucuggggag ccaggaagga gccguucaca cggcucucgc uggagcucua     1080

gaggcugaga uggauggugc aaagggaagg cuguucucug gccauuugaa augccgccua     1140

aaaauggaca agcucagauu gaagggcgug ucauauuccu ugugcacugc ggcauucaca     1200

uucaccaagg ucccagcuga aacacugcau ggaacaguca caguggaggu gcaguaugca     1260

gggacagaug gacccugcaa gaucccaguc cagauggcgg uggacaugca gacccugacc     1320

ccaguuggaa ggcugauaac cgccaacccc gugauuacug aaagcacuga gaacucaaag     1380

augauguugg agcuugaccc accauuuggg gauucuuaca uugucauagg aguuggggac     1440

aagaaaauca cccaccacug gcauaggagu gguagcacca ucggaaaggc auuugaggcc     1500

acugugagag gcgccaagag aauggcaguc cugggggaua cagccuggga cuucggauca     1560

gucgggggug uguucaacuc acuggguaag ggcauucacc agauuuuugg agcagccuuc     1620

aaaucacugu uuggaggaau guccugguuc ucacagaucc ucauaggcac gcugcuagug     1680

ugguuagguu ugaacacaaa gaauggaucu aucucccuca caugcuuggc ccugggggga     1740

gugaugaucu uccucuccac ggcuguuucu gcu                                  1773


<210>  9708
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS wild type

<400>  9708
augaccagug ucggaauugu uggccuccug cugaccacag cuauggcagc ugugacgcuc       60

cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa      120

uacacaaagc acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua      180

gcagcagcug ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug      240

gucaugauac ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg      300

gacuuugugg aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu      360

ugugucaccg uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca      420

gucagcaaca uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu      480

ucggacagcc gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa      540

uaugucugca aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc      600

aaagggagcc uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc      660

auccagccag agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu      720

gggaugaucg uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua      780

acgcccaauu caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau      840

ugugaaccga ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag      900

cacugguugg uccacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca      960

gacaccggaa cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau     1020

gccaaaaggc aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu     1080

gcuggagcuc uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug     1140

aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc     1200

gcagcguuca cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag     1260

guacaguacg cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug     1320

caaacucuga ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu     1380

gagaacucua agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua     1440

ggagucgggg agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa     1500

gcauuugaag ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg     1560

gacuuuggau caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu     1620

ggagcagcuu ucaaaucauu guuuggagga auguccuggu ucucacaaau ccucauugga     1680

acguugcuga ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug     1740

gccuuagggg gaguguugau cuucuuaucc acagccgucu cugcu                     1785


<210>  9709
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS wild type

<400>  9709
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      120

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      180

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      240

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      300

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      360

guugauauug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      420

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      480

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      540

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      600

uggggaaaug gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca      660

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug      720

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu      780

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug      840

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau      900

uuguauuacu ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac      960

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1020

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1080

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1140

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1200

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1260

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1320

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1380

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1440

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1500

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1560

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1620

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1680

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag     1740

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     1800

gccgucucug cu                                                         1812


<210>  9710
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS wild type

<400>  9710
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      120

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      180

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      240

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      300

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      360

guugauguug ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu      420

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      480

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      540

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      600

uggggaaaug gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca      660

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug      720

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu      780

gaugagaaua gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug      840

gggggguuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau      900

uuguauuacu ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac      960

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1020

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1080

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1140

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1200

aagggcgugu cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa     1260

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1320

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1380

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1440

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1500

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1560

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1620

uugggcaagg gcauccauca aaucuuugga gcagcuuuca aaucauuguu uggaggaaug     1680

uccugguucu cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacgcaaag     1740

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     1800

gccgucucug cu                                                         1812


<210>  9711
<211>  1800
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS wild type

<400>  9711
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg      120

ucgcagaccu gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg      180

auauucagga accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc      240

ucgacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu      300

aucaggugca uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg      360

guugauguug ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca      420

gucgacauag aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc      480

uacgaggcau cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc      540

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu      600

uggggaaacg guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg      660

uguucuaaga agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug      720

cuaucagugc auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga      780

gcgaaagucg agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga      840

aguuuaggac uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug      900

accaugaaca auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu      960

uggcaugcug gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa     1020

uuuaaggaug cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc     1080

guucacacgg cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug     1140

uucucuggcc auuugaaaug ccgccuaaaa auggacaagc ucagauugaa gggcguguca     1200

uauuccuugu gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga     1260

acagucacag uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag     1320

auggcggugg acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug     1380

auuacugaaa gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau     1440

ucuuacauug ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu     1500

agcaccaucg gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug     1560

ggggauacag ccugggacuu cggaucaguc gggggugugu ucaacucacu ggguaagggc     1620

auucaccaga uuuuuggagc agccuucaaa ucacuguuug gaggaauguc cugguucuca     1680

cagauccuca uaggcacgcu gcuagugugg uuagguuuga acacaaagaa uggaucuauc     1740

ucccucacau gcuuggcccu ggggggagug augaucuucc ucuccacggc uguuucugcu     1800


<210>  9712
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS wild type

<400>  9712
auggccauaa guggaguccc ugugcuagga uuuuucauca uagcugugcu gaugagcgcu       60

caggaaucau gggcugcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg      120

ucgcaaaccu gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg      180

auauucagga acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc      240

ucaacgagcc aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc      300

aucaggugca uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg      360

guugauguug ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu      420

gucgacauag agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc      480

uaugaggcau caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc      540

uaccuugaca agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc      600

uggggaaaug gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca      660

ugcuccaaga aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug      720

cugucaguuc auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu      780

gaugagaaua gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug      840

ggggguuuug gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau      900

uuguauuacu ugacuaugaa uaacaagcac ugguuggucc acaaggagug guuccacgac      960

auuccauuac cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa     1020

gcacugguag aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu     1080

caagaaggag caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca     1140

aagggaaggc uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug     1200

aagggcgugu cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa     1260

acacugcacg ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag     1320

guuccagcuc agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc     1380

gcuaaccccg uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca     1440

ccauuugggg acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg     1500

cacaggagug gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga     1560

auggcagucu ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca     1620

uugggcaagg gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug     1680

uccugguucu cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag     1740

aauggaucua uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca     1800

gccgucucug cu                                                         1812


<210>  9713
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS wild type

<400>  9713
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgcugug       60

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      120

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      180

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      240

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      300

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauauugu cuuggaacau      360

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      420

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      480

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      540

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      600

uuuggcaaag ggagucuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      660

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag      720

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu      780

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga      840

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau      900

aacaagcacu gguugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu      960

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1020

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1080

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1140

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1200

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1260

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1320

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1380

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1440

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1500

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1560

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1620

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1680

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     1740

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc u              1791


<210>  9714
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS wild type

<400>  9714
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgcugug       60

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      120

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      180

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      240

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      300

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauguugu cuuggaacau      360

ggagguugug ucacuguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      420

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      480

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      540

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      600

uuuggcaaag ggagccuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      660

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag      720

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaaaguu      780

gagauaacgc ccaauucacc aagagccgaa gccacccugg ggggguuugg aagccuagga      840

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau      900

aacaagcacu ggcugguuca caaggagugg uuccacgaca uuccauuacc uuggcacgcu      960

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1020

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1080

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1140

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1200

uguacugcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1260

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1320

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1380

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1440

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1500

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1560

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1620

aucuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauucuc     1680

auuggaacgu ugcugaugug guugggucug aacgcaaaga auggaucuau uucccuuaug     1740

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc u              1791


<210>  9715
<211>  1779
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS wild type

<400>  9715
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgccgug       60

acgcucccuu cucacucuac aaggaaguug caaacgcggu cgcagaccug guuagaauca      120

agagaauaca cgaagcacuu gaucaagguu gaaaacugga uauucaggaa ccccggguuu      180

gcgcuagugg ccguugccau ugccuggcuu uugggaagcu cgacgagcca aaaagucaua      240

uacuugguca ugauacugcu gauugccccg gcauacagua ucaggugcau uggagucagc      300

aauagagacu ucguggaggg caugucaggu gggaccuggg uugauguugu cuuggaacau      360

ggaggcugcg uuaccgugau ggcacaggac aagccaacag ucgacauaga guuggucacg      420

acgacgguua guaacauggc cgagguaaga uccuauugcu acgaggcauc gauaucggac      480

auggcuucgg acagucguug cccaacacaa ggugaagccu accuugacaa gcaaucagac      540

acucaauaug ucugcaaaag aacauuagug gacagagguu ggggaaacgg uuguggacuu      600

uuuggcaaag ggagcuuggu gacaugugcc aaguuuacgu guucuaagaa gaugaccggg      660

aagagcauuc aaccggaaaa ucuggaguau cggauaaugc uaucagugca uggcucccag      720

cauagcggga ugauuggaua ugaaacugac gaagauagag cgaaagucga gguuacgccu      780

aauucaccaa gagcggaagc aaccuuggga ggcuuuggaa guuuaggacu ugacugugaa      840

ccaaggacag gccuugacuu uucagaucug uauuaccuga ccaugaacaa uaagcauugg      900

uuggugcaca aagagugguu ucaugacauc ccauugccuu ggcaugcugg ggcagacacc      960

ggaacuccac acuggaacaa caaagaggca uugguagaau uuaaggaugc ccacgccaag     1020

aggcaaaccg ucgucguucu ggggagccag gaaggagccg uucacacggc ucucgcugga     1080

gcucuagagg cugagaugga uggugcaaag ggaaggcugu ucucuggcca uuugaaaugc     1140

cgccuaaaaa uggacaagcu cagauugaag ggcgugucau auuccuugug cacugcggca     1200

uucacauuca ccaagguccc agcugaaaca cugcauggaa cagucacagu ggaggugcag     1260

uaugcaggga cagauggacc cugcaagauc ccaguccaga uggcggugga caugcagacc     1320

cugaccccag uuggaaggcu gauaaccgcc aaccccguga uuacugaaag cacugagaac     1380

ucaaagauga uguuggagcu ugacccacca uuuggggauu cuuacauugu cauaggaguu     1440

ggggacaaga aaaucaccca ccacuggcau aggaguggua gcaccaucgg aaaggcauuu     1500

gaggccacug ugagaggcgc caagagaaug gcaguccugg gggauacagc cugggacuuc     1560

ggaucagucg gggguguguu caacucacug gguaagggca uucaccagau uuuuggagca     1620

gccuucaaau cacuguuugg aggaaugucc ugguucucac agauccucau aggcacgcug     1680

cuaguguggu uagguuugaa cacaaagaau ggaucuaucu cccucacaug cuuggcccug     1740

gggggaguga ugaucuuccu cuccacggcu guuucugcu                            1779


<210>  9716
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS wild type

<400>  9716
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgcugug       60

acgcuccccu cccauuccac uaggaagcug caaacgcggu cgcaaaccug guuggaauca      120

agagaauaca caaagcacuu gauuagaguc gaaaauugga uauucaggaa cccuggcuuc      180

gcguuagcag cagcugccau cgcuuggcuu uugggaagcu caacgagcca aaaagucaua      240

uacuugguca ugauacugcu gauugccccg gcauacagca ucaggugcau aggagucagc      300

aauagggacu uuguggaagg uaugucaggu gggacuuggg uugauguugu cuuggaacau      360

ggagguugug ucaccguaau ggcacaggac aaaccgacug ucgacauaga gcugguuaca      420

acaacaguca gcaacauggc ggagguaaga uccuacugcu augaggcauc aauaucagac      480

auggcuucgg acagccgcug cccaacacaa ggugaagccu accuugacaa gcaaucagac      540

acucaauaug ucugcaaaag aacguuagug gacagaggcu ggggaaaugg auguggacuu      600

uuuggcaaag ggagccuggu gacaugcgcu aaguuugcau gcuccaagaa aaugaccggg      660

aagagcaucc agccagagaa ucuggaguac cggauaaugc ugucaguuca uggcucccag      720

cacaguggga ugaucguuaa ugacacagga caugaaacug augagaauag agcgaagguu      780

gagauaacgc ccaauucacc aagagccgaa gccacccugg gggguuuugg aagccuagga      840

cuugauugug aaccgaggac aggccuugac uuuucagauu uguauuacuu gacuaugaau      900

aacaagcacu gguuggucca caaggagugg uuccacgaca uuccauuacc uuggcacgcu      960

ggggcagaca ccggaacucc acacuggaac aacaaagaag cacugguaga guucaaggac     1020

gcacaugcca aaaggcaaac ugucgugguu cuagggaguc aagaaggagc aguucacacg     1080

gcccuugcug gagcucugga ggcugagaug gauggugcaa agggaaggcu guccucuggc     1140

cacuugaaau gucgccugaa aauggauaaa cuuagauuga agggcguguc auacuccuug     1200

uguaccgcag cguucacauu caccaagauc ccggcugaaa cacugcacgg gacagucaca     1260

guggagguac aguacgcagg gacagaugga ccuugcaagg uuccagcuca gauggcggug     1320

gacaugcaaa cucugacccc aguugggagg uugauaaccg cuaaccccgu aaucacugaa     1380

agcacugaga acucuaagau gaugcuggaa cuugauccac cauuugggga cucuuacauu     1440

gucauaggag ucggggagaa gaagaucacc caccacuggc acaggagugg cagcaccauu     1500

ggaaaagcau uugaagccac ugugagaggu gccaagagaa uggcagucuu gggagacaca     1560

gccugggacu uuggaucagu uggaggcgcu cucaacucau ugggcaaggg cauccaucaa     1620

auuuuuggag cagcuuucaa aucauuguuu ggaggaaugu ccugguucuc acaaauccuc     1680

auuggaacgu ugcugaugug guugggucug aacacaaaga auggaucuau uucccuuaug     1740

ugcuuggccu uagggggagu guugaucuuc uuauccacag ccgucucugc u              1791


<210>  9717
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS wild type

<400>  9717
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      120

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      180

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      240

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      300

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      360

gauauugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      420

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      480

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      540

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      600

ggaaauggau guggacuuuu uggcaaaggg agucugguga caugcgcuaa guuugcaugc      660

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug      720

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau      780

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg      840

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug      900

uauuacuuga cuaugaauaa caagcacugg uugguucaca aggagugguu ccacgacauu      960

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1020

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1080

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1140

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1200

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1260

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1320

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1380

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1440

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1500

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1560

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1620

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1680

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cacaaagaau     1740

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     1800

gucucugcu                                                             1809


<210>  9718
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS wild type

<400>  9718
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      120

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      180

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      240

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      300

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      360

gauguugucu uggaacaugg agguuguguc acuguaaugg cacaggacaa accgacuguc      420

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      480

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      540

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      600

ggaaauggau guggacuuuu uggcaaaggg agccugguga caugcgcuaa guuugcaugc      660

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug      720

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau      780

gagaauagag cgaaaguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg      840

ggguuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug      900

uauuacuuga cuaugaauaa caagcacugg cugguucaca aggagugguu ccacgacauu      960

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1020

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1080

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1140

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1200

ggcgugucau acuccuugug uacugcagcg uucacauuca ccaagauccc ggcugaaaca     1260

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1320

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1380

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1440

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1500

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1560

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1620

ggcaagggca uccaucaaau cuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1680

ugguucucac aaauucucau uggaacguug cugauguggu ugggucugaa cgcaaagaau     1740

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     1800

gucucugcu                                                             1809


<210>  9719
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS wild type

<400>  9719
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgccgugac gcucccuucu cacucuacaa ggaaguugca aacgcggucg      120

cagaccuggu uagaaucaag agaauacacg aagcacuuga ucaagguuga aaacuggaua      180

uucaggaacc ccggguuugc gcuaguggcc guugccauug ccuggcuuuu gggaagcucg      240

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacaguauc      300

aggugcauug gagucagcaa uagagacuuc guggagggca ugucaggugg gaccuggguu      360

gauguugucu uggaacaugg aggcugcguu accgugaugg cacaggacaa gccaacaguc      420

gacauagagu uggucacgac gacgguuagu aacauggccg agguaagauc cuauugcuac      480

gaggcaucga uaucggacau ggcuucggac agucguugcc caacacaagg ugaagccuac      540

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cauuagugga cagagguugg      600

ggaaacgguu guggacuuuu uggcaaaggg agcuugguga caugugccaa guuuacgugu      660

ucuaagaaga ugaccgggaa gagcauucaa ccggaaaauc uggaguaucg gauaaugcua      720

ucagugcaug gcucccagca uagcgggaug auuggauaug aaacugacga agauagagcg      780

aaagucgagg uuacgccuaa uucaccaaga gcggaagcaa ccuugggagg cuuuggaagu      840

uuaggacuug acugugaacc aaggacaggc cuugacuuuu cagaucugua uuaccugacc      900

augaacaaua agcauugguu ggugcacaaa gagugguuuc augacauccc auugccuugg      960

caugcugggg cagacaccgg aacuccacac uggaacaaca aagaggcauu gguagaauuu     1020

aaggaugccc acgccaagag gcaaaccguc gucguucugg ggagccagga aggagccguu     1080

cacacggcuc ucgcuggagc ucuagaggcu gagauggaug gugcaaaggg aaggcuguuc     1140

ucuggccauu ugaaaugccg ccuaaaaaug gacaagcuca gauugaaggg cgugucauau     1200

uccuugugca cugcggcauu cacauucacc aaggucccag cugaaacacu gcauggaaca     1260

gucacagugg aggugcagua ugcagggaca gauggacccu gcaagauccc aguccagaug     1320

gcgguggaca ugcagacccu gaccccaguu ggaaggcuga uaaccgccaa ccccgugauu     1380

acugaaagca cugagaacuc aaagaugaug uuggagcuug acccaccauu uggggauucu     1440

uacauuguca uaggaguugg ggacaagaaa aucacccacc acuggcauag gagugguagc     1500

accaucggaa aggcauuuga ggccacugug agaggcgcca agagaauggc aguccugggg     1560

gauacagccu gggacuucgg aucagucggg gguguguuca acucacuggg uaagggcauu     1620

caccagauuu uuggagcagc cuucaaauca cuguuuggag gaauguccug guucucacag     1680

auccucauag gcacgcugcu agugugguua gguuugaaca caaagaaugg aucuaucucc     1740

cucacaugcu uggcccuggg gggagugaug aucuuccucu ccacggcugu uucugcu        1797


<210>  9720
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS wild type

<400>  9720
auggguaaac ggagcgcugg cucaaucaug uggcucgcga gcuuggcagu ugucauagcu       60

ugugcaggag ccgcugugac gcuccccucc cauuccacua ggaagcugca aacgcggucg      120

caaaccuggu uggaaucaag agaauacaca aagcacuuga uuagagucga aaauuggaua      180

uucaggaacc cuggcuucgc guuagcagca gcugccaucg cuuggcuuuu gggaagcuca      240

acgagccaaa aagucauaua cuuggucaug auacugcuga uugccccggc auacagcauc      300

aggugcauag gagucagcaa uagggacuuu guggaaggua ugucaggugg gacuuggguu      360

gauguugucu uggaacaugg agguuguguc accguaaugg cacaggacaa accgacuguc      420

gacauagagc ugguuacaac aacagucagc aacauggcgg agguaagauc cuacugcuau      480

gaggcaucaa uaucagacau ggcuucggac agccgcugcc caacacaagg ugaagccuac      540

cuugacaagc aaucagacac ucaauauguc ugcaaaagaa cguuagugga cagaggcugg      600

ggaaauggau guggacuuuu uggcaaaggg agccugguga caugcgcuaa guuugcaugc      660

uccaagaaaa ugaccgggaa gagcauccag ccagagaauc uggaguaccg gauaaugcug      720

ucaguucaug gcucccagca cagugggaug aucguuaaug acacaggaca ugaaacugau      780

gagaauagag cgaagguuga gauaacgccc aauucaccaa gagccgaagc cacccugggg      840

gguuuuggaa gccuaggacu ugauugugaa ccgaggacag gccuugacuu uucagauuug      900

uauuacuuga cuaugaauaa caagcacugg uugguccaca aggagugguu ccacgacauu      960

ccauuaccuu ggcacgcugg ggcagacacc ggaacuccac acuggaacaa caaagaagca     1020

cugguagagu ucaaggacgc acaugccaaa aggcaaacug ucgugguucu agggagucaa     1080

gaaggagcag uucacacggc ccuugcugga gcucuggagg cugagaugga uggugcaaag     1140

ggaaggcugu ccucuggcca cuugaaaugu cgccugaaaa uggauaaacu uagauugaag     1200

ggcgugucau acuccuugug uaccgcagcg uucacauuca ccaagauccc ggcugaaaca     1260

cugcacggga cagucacagu ggagguacag uacgcaggga cagauggacc uugcaagguu     1320

ccagcucaga uggcggugga caugcaaacu cugaccccag uugggagguu gauaaccgcu     1380

aaccccguaa ucacugaaag cacugagaac ucuaagauga ugcuggaacu ugauccacca     1440

uuuggggacu cuuacauugu cauaggaguc ggggagaaga agaucaccca ccacuggcac     1500

aggaguggca gcaccauugg aaaagcauuu gaagccacug ugagaggugc caagagaaug     1560

gcagucuugg gagacacagc cugggacuuu ggaucaguug gaggcgcucu caacucauug     1620

ggcaagggca uccaucaaau uuuuggagca gcuuucaaau cauuguuugg aggaaugucc     1680

ugguucucac aaauccucau uggaacguug cugauguggu ugggucugaa cacaaagaau     1740

ggaucuauuu cccuuaugug cuuggccuua gggggagugu ugaucuucuu auccacagcc     1800

gucucugcu                                                             1809


<210>  9721
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9721
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgcc                     2085


<210>  9722
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9722
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacac gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccacgagcac cugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacgcgcaa gcugcagacc cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacga gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacc uggguggacg ucgugcugga gcacgggggc      660

ugcgugaccg ucauggccca ggacaagccc accguggaca ucgagcucgu gacgaccacc      720

gucagcaaca uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacgcag      840

uacgucugca agcgcacccu gguggaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      960

auccagccgg agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc     1020

gggaugaucg ugaacgacac gggccacgag accgacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggacgggccu ggacuucagc gaccuguacu accucaccau gaacaacaag     1200

cacuggcugg uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac     1320

gccaagcgcc agacgguggu cgugcugggc ucccaggagg gggccgugca caccgcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacgcucc acggcaccgu gaccguggag     1560

guccaguacg ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug     1620

cagacgcuga cccccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacg     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcgucgggg agaagaagau cacccaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccgugcg cggcgccaag cggauggccg ugcuggggga cacggcgugg     1860

gacuucggca gcgucggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acccugcuga uguggcucgg gcugaacgcc aagaacggcu ccaucagccu gaugugccuc     2040

gcgcugggcg gcgugcugau cuuccucucc accgccguga gcgcc                     2085


<210>  9723
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9723
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccaucac ccgccggggg uccgccuacu acauguaccu ggaccgcagc      120

gacgccggca aggccaucuc cuucgcgacc acgcugggcg ugaacaagug ccacgugcag      180

aucauggacc ucgggcacau gugcgacgcc accaugagcu acgagugccc gaugcuggac      240

gagggcgucg agcccgacga cguggacugc uggugcaaca ccaccuccac gugggugguc      300

uacgggaccu gccaccacaa gaagggcgag gcccggcgca gccggcgcgc cgugacccug      360

cccucccaca gcacccggaa gcuccagacg cgcucccaga ccuggcugga gagccgcgag      420

uacaccaagc accugaucaa gguggagaac uggaucuucc ggaaccccgg cuucgcccuc      480

gucgcggugg ccaucgccug gcugcugggg uccagcaccu cccagaaggu gaucuaccuc      540

gucaugaucc ugcugaucgc cccggccuac agcauccgcu gcaucggcgu guccaaccgg      600

gacuucgugg aggggaugag cggcgggacg ugggucgacg uggugcucga gcacggcggc      660

ugcgucaccg ugauggcgca ggacaagccc accguggaca ucgagcuggu caccacgacc      720

guguccaaca uggccgaggu ccgcagcuac ugcuacgagg ccagcaucuc cgacauggcc      780

agcgacuccc ggugccccac ccagggggag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agcgcacgcu cguggaccgg ggcuggggga acggcugcgg gcuguucggc      900

aagggcuccc uggucacgug cgcgaaguuc acgugcagca agaagaugac cgggaagucc      960

auccagcccg agaaccucga guaccgcauc augcugagcg ugcacggcuc ccagcacagc     1020

gggaugaucg gcuacgagac ggacgaggac cgggccaagg uggaggucac cccgaacucc     1080

ccgcgcgccg aggccacccu gggcggguuc ggcagccucg ggcuggacug cgagccccgg     1140

accggccugg acuucuccga ccucuacuac cugacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccuc ccguggcacg cgggggccga caccggcacc     1260

ccgcacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagcgccag     1320

accgucgugg ugcugggcag ccaggagggg gcgguccaca cggcccuggc cggcgcccuc     1380

gaggccgaga uggacggggc gaagggccgg cuguucuccg ggcaccugaa gugccgccuc     1440

aagauggaca agcugcggcu gaagggcgug agcuacuccc ucugcaccgc cgccuucacc     1500

uucaccaagg ugcccgccga gacccugcac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gccccugcaa gaucccgguc cagauggcgg uggacaugca gacccugacg     1620

cccguggggc gccucaucac cgccaacccc gucaucaccg agagcaccga gaacuccaag     1680

augaugcugg agcuggaccc gccguucggc gacagcuaca ucgugaucgg ggugggcgac     1740

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1800

accguccgcg gcgcgaagcg gauggccgug cucggggaca ccgccuggga cuucggcagc     1860

gugggcggcg ucuucaacuc ccuggggaag gggauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucgggac gcugcuggug     1980

uggcucggcc ugaacaccaa gaacggcucc aucagccuga ccugccucgc gcugggcggc     2040

gugaugaucu uccuguccac cgccgucagc gcc                                  2073


<210>  9724
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9724
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggacg ucgugcugga gcacgggggc      660

ugcgugaccg ucauggccca ggacaagccc accguggaca ucgagcucgu gaccacgacc      720

gucagcaaca uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgucugca agcgcacgcu gguggaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      960

auccagccgg agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc     1020

gggaugaucg ugaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu cgugcugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu gaccguggag     1560

guccaguacg ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cgcccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcgucgggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccgugcg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1860

gacuucggca gcgucggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1980

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     2040

gcccugggcg gcgugcugau cuuccucucc accgcgguga gcgcc                     2085


<210>  9725
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9725
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacc       60

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      360

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      660

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      840

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1020

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1140

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1320

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1560

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1620

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1740

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1800

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1860

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1920

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     1980

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     2040

uuccucucca ccgcggugag cgcc                                            2064


<210>  9726
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9726
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacc       60

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacacg      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac cacgagcacc ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacgcgcaag      360

cugcagaccc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccacgag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      660

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      840

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg     1020

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug     1140

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc     1320

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1560

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1620

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1740

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1800

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cgucggcggg     1860

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1920

uucgggggca uguccugguu cagccagauc cucaucggca cccugcugau guggcucggg     1980

cugaacgcca agaacggcuc caucagccug augugccucg cgcugggcgg cgugcugauc     2040

uuccucucca ccgccgugag cgcc                                            2064


<210>  9727
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9727
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcaucacc       60

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      120

uucgcgacca cgcugggcgu gaacaagugc cacgugcaga ucauggaccu cgggcacaug      180

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      240

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      300

aagggcgagg cccggcgcag ccggcgcgcc gugacccugc ccucccacag cacccggaag      360

cuccagacgc gcucccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      420

guggagaacu ggaucuuccg gaaccccggc uucgcccucg ucgcgguggc caucgccugg      480

cugcuggggu ccagcaccuc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc      540

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      600

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      660

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      720

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      780

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      840

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      900

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg     1020

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug     1080

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac     1140

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1200

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag     1260

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc     1320

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg     1380

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug     1440

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1500

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1560

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1620

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1680

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1740

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1800

auggccgugc ucggggacac cgccugggac uucggcagcg ugggcggcgu cuucaacucc     1860

cuggggaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1920

uccugguuca gccagauccu caucgggacg cugcuggugu ggcucggccu gaacaccaag     1980

aacggcucca ucagccugac cugccucgcg cugggcggcg ugaugaucuu ccuguccacc     2040

gccgucagcg cc                                                         2052


<210>  9728
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9728
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacc       60

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      360

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      660

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      840

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1020

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1140

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc     1320

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1560

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1620

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1740

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1800

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgucggcggg     1860

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1920

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     1980

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cgugcugauc     2040

uuccucucca ccgcggugag cgcc                                            2064


<210>  9729
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9729
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ugggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg uccugaucuu ccucuccacc gcggugagcg cc                        2082


<210>  9730
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9730
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacacgug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca cgagcaccug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cgcgcaagcu gcagacccgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccacgagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcaccugg guggacgucg ugcuggagca cgggggcugc      660

gugaccguca uggcccagga caagcccacc guggacaucg agcucgugac gaccaccguc      720

agcaacaugg ccgaggugcg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacgcaguac      840

gucugcaagc gcacccuggu ggaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc      960

cagccggaga accuggagua ccgcaucaug cucuccgucc acggcagcca gcacuccggg     1020

augaucguga acgacacggg ccacgagacc gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga cgggccugga cuucagcgac cuguacuacc ucaccaugaa caacaagcac     1200

uggcuggucc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgugg aguucaagga cgcgcacgcc     1320

aagcgccaga cgguggucgu gcugggcucc caggaggggg ccgugcacac cgcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguca gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acgcuccacg gcaccgugac cguggagguc     1560

caguacgccg ggaccgacgg cccgugcaag gugcccgccc agauggccgu ggacaugcag     1620

acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg ugaucaccga gagcacggag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gucggggaga agaagaucac ccaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc ugggggacac ggcgugggac     1860

uucggcagcg ucggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacc     1980

cugcugaugu ggcucgggcu gaacgccaag aacggcucca ucagccugau gugccucgcg     2040

cugggcggcg ugcugaucuu ccucuccacc gccgugagcg cc                        2082


<210>  9731
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9731
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccaucacccg ccgggggucc gccuacuaca uguaccugga ccgcagcgac      120

gccggcaagg ccaucuccuu cgcgaccacg cugggcguga acaagugcca cgugcagauc      180

auggaccucg ggcacaugug cgacgccacc augagcuacg agugcccgau gcuggacgag      240

ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca ccuccacgug gguggucuac      300

gggaccugcc accacaagaa gggcgaggcc cggcgcagcc ggcgcgccgu gacccugccc      360

ucccacagca cccggaagcu ccagacgcgc ucccagaccu ggcuggagag ccgcgaguac      420

accaagcacc ugaucaaggu ggagaacugg aucuuccgga accccggcuu cgcccucguc      480

gcgguggcca ucgccuggcu gcuggggucc agcaccuccc agaaggugau cuaccucguc      540

augauccugc ugaucgcccc ggccuacagc auccgcugca ucggcguguc caaccgggac      600

uucguggagg ggaugagcgg cgggacgugg gucgacgugg ugcucgagca cggcggcugc      660

gucaccguga uggcgcagga caagcccacc guggacaucg agcuggucac cacgaccgug      720

uccaacaugg ccgagguccg cagcuacugc uacgaggcca gcaucuccga cauggccagc      780

gacucccggu gccccaccca gggggaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaagc gcacgcucgu ggaccggggc ugggggaacg gcugcgggcu guucggcaag      900

ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga agaugaccgg gaaguccauc      960

cagcccgaga accucgagua ccgcaucaug cugagcgugc acggcuccca gcacagcggg     1020

augaucggcu acgagacgga cgaggaccgg gccaaggugg aggucacccc gaacuccccg     1080

cgcgccgagg ccacccuggg cggguucggc agccucgggc uggacugcga gccccggacc     1140

ggccuggacu ucuccgaccu cuacuaccug acgaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccucccg uggcacgcgg gggccgacac cggcaccccg     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1320

gucguggugc ugggcagcca ggagggggcg guccacacgg cccuggccgg cgcccucgag     1380

gccgagaugg acggggcgaa gggccggcug uucuccgggc accugaagug ccgccucaag     1440

auggacaagc ugcggcugaa gggcgugagc uacucccucu gcaccgccgc cuucaccuuc     1500

accaaggugc ccgccgagac ccugcacggc acggucaccg uggaggugca guacgccggg     1560

accgacggcc ccugcaagau cccgguccag auggcggugg acaugcagac ccugacgccc     1620

guggggcgcc ucaucaccgc caaccccguc aucaccgaga gcaccgagaa cuccaagaug     1680

augcuggagc uggacccgcc guucggcgac agcuacaucg ugaucggggu gggcgacaag     1740

aagaucacgc accacuggca ccggagcggc uccaccaucg ggaaggccuu cgaggccacc     1800

guccgcggcg cgaagcggau ggccgugcuc ggggacaccg ccugggacuu cggcagcgug     1860

ggcggcgucu ucaacucccu ggggaagggg auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg ggggcauguc cugguucagc cagauccuca ucgggacgcu gcuggugugg     1980

cucggccuga acaccaagaa cggcuccauc agccugaccu gccucgcgcu gggcggcgug     2040

augaucuucc uguccaccgc cgucagcgcc                                      2070


<210>  9732
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9732
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacgucg ugcuggagca cgggggcugc      660

gugaccguca uggcccagga caagcccacc guggacaucg agcucgugac cacgaccguc      720

agcaacaugg ccgaggugcg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gucugcaagc gcacgcuggu ggaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc      960

cagccggaga accuggagua ccgcaucaug cucuccgucc acggcagcca gcacuccggg     1020

augaucguga acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggucc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgugg aguucaagga cgcgcacgcc     1320

aagcgccaga ccguggucgu gcugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguca gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggugac cguggagguc     1560

caguacgccg ggaccgacgg cccgugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugacgc ccgucgggcg ccugaucacc gcgaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gucggggaga agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc     1800

uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc ugggggacac cgcgugggac     1860

uucggcagcg ucggcggggc ccucaacucc cugggcaagg ggauccacca gaucuucggc     1920

gccgccuuca agagccuguu cgggggcaug uccugguuca gccagauccu caucggcacg     1980

cugcugaugu ggcucgggcu gaacaccaag aacggcucca ucagccugau gugccucgcc     2040

cugggcggcg ugcugaucuu ccucuccacc gcggugagcg cc                        2082


<210>  9733
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9733
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      660

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      720

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      960

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc     1020

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1620

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcagcac ccuggggaag     1800

gccuucucca ccacgcucaa gggcgcccag cgccuggcgg cccuggggga caccgccugg     1860

gacuucggca gcaucggggg cguguucaac uccaucggcc gggccgucca ccagguguuc     1920

gggggcgccu uccgcacccu cuucgggggc augagcugga ucacccaggg gcugaugggc     1980

gcgcugcucc uguggauggg ggugaacgcc cgggaccgcu ccaucgcccu ggccuuccuc     2040

gcgacgggcg gcguccuggu guuccuggcc accaacgugc acgcc                     2085


<210>  9734
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9734
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacac gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccacgagcac cugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacgcgcaa gcugcagacc cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacga gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacc uggguggacg ucgugcugga gcacgggggc      660

ugcgugaccg ucauggccca ggacaagccc accguggaca ucgagcucgu gacgaccacc      720

gucagcaaca uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacgcag      840

uacgucugca agcgcacccu gguggaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      960

auccagccgg agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc     1020

gggaugaucg ugaacgacac gggccacgag accgacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggacgggccu ggacuucagc gaccuguacu accucaccau gaacaacaag     1200

cacuggcugg uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac     1320

gccaagcgcc agacgguggu cgugcugggc ucccaggagg gggccgugca caccgcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacgcucc acggcaccgu gaccguggag     1560

guccaguacg ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug     1620

cagacgcuga cccccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacg     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcgucgggg agaagaagau cacccaccac uggcaccgga gcggcagcac ccuggggaag     1800

gccuucucca ccacgcucaa gggcgcccag cgccuggcgg cccuggggga caccgccugg     1860

gacuucggca gcaucggggg cguguucaac uccaucggcc gggccgucca ccagguguuc     1920

gggggcgccu uccgcacccu cuucgggggc augagcugga ucacccaggg gcugaugggc     1980

gcgcugcucc uguggauggg ggugaacgcc cgggaccgcu ccaucgcccu ggccuuccuc     2040

gcgacgggcg gcguccuggu guuccuggcc accaacgugc acgcc                     2085


<210>  9735
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9735
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccaucac ccgccggggg uccgccuacu acauguaccu ggaccgcagc      120

gacgccggca aggccaucuc cuucgcgacc acgcugggcg ugaacaagug ccacgugcag      180

aucauggacc ucgggcacau gugcgacgcc accaugagcu acgagugccc gaugcuggac      240

gagggcgucg agcccgacga cguggacugc uggugcaaca ccaccuccac gugggugguc      300

uacgggaccu gccaccacaa gaagggcgag gcccggcgca gccggcgcgc cgugacccug      360

cccucccaca gcacccggaa gcuccagacg cgcucccaga ccuggcugga gagccgcgag      420

uacaccaagc accugaucaa gguggagaac uggaucuucc ggaaccccgg cuucgcccuc      480

gucgcggugg ccaucgccug gcugcugggg uccagcaccu cccagaaggu gaucuaccuc      540

gucaugaucc ugcugaucgc cccggccuac agcauccgcu gcaucggcgu guccaaccgg      600

gacuucgugg aggggaugag cggcgggacg ugggucgacg uggugcucga gcacggcggc      660

ugcgucaccg ugauggcgca ggacaagccc accguggaca ucgagcuggu caccacgacc      720

guguccaaca uggccgaggu ccgcagcuac ugcuacgagg ccagcaucuc cgacauggcc      780

agcgacuccc ggugccccac ccagggggag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agcgcacgcu cguggaccgg ggcuggggga acggcugcgg gcuguucggc      900

aagggcuccc uggucacgug cgcgaaguuc acgugcagca agaagaugac cgggaagucc      960

auccagcccg agaaccucga guaccgcauc augcugagcg ugcacggcuc ccagcacagc     1020

gggaugaucg gcuacgagac ggacgaggac cgggccaagg uggaggucac cccgaacucc     1080

ccgcgcgccg aggccacccu gggcggguuc ggcagccucg ggcuggacug cgagccccgg     1140

accggccugg acuucuccga ccucuacuac cugacgauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccuc ccguggcacg cgggggccga caccggcacc     1260

ccgcacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagcgccag     1320

accgucgugg ugcugggcag ccaggagggg gcgguccaca cggcccuggc cggcgcccuc     1380

gaggccgaga uggacggggc gaagggccgg cuguucuccg ggcaccugaa gugccgccuc     1440

aagauggaca agcugcggcu gaagggcgug agcuacuccc ucugcaccgc cgccuucacc     1500

uucaccaagg ugcccgccga gacccugcac ggcacgguca ccguggaggu gcaguacgcc     1560

gggaccgacg gccccugcaa gaucccgguc cagauggcgg uggacaugca gacccugacg     1620

cccguggggc gccucaucac cgccaacccc gucaucaccg agagcaccga gaacuccaag     1680

augaugcugg agcuggaccc gccguucggc gacagcuaca ucgugaucgg ggugggcgac     1740

aagaagauca cgcaccacug gcaccggagc ggcagcaccc uggggaaggc cuucuccacc     1800

acgcucaagg gcgcccagcg ccuggcggcc cugggggaca ccgccuggga cuucggcagc     1860

aucgggggcg uguucaacuc caucggccgg gccguccacc agguguucgg gggcgccuuc     1920

cgcacccucu ucgggggcau gagcuggauc acccaggggc ugaugggcgc gcugcuccug     1980

uggauggggg ugaacgcccg ggaccgcucc aucgcccugg ccuuccucgc gacgggcggc     2040

guccuggugu uccuggccac caacgugcac gcc                                  2073


<210>  9736
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9736
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac      120

gacgccgggg aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag      180

aucauggacc uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug      300

uacggcacgu gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug      360

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      420

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      480

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      540

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      600

gacuucgugg aggggauguc cggcggcacg uggguggacg ucgugcugga gcacgggggc      660

ugcgugaccg ucauggccca ggacaagccc accguggaca ucgagcucgu gaccacgacc      720

gucagcaaca uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc      780

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      840

uacgucugca agcgcacgcu gguggaccgg ggcuggggga acggcugcgg ccucuucggg      900

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      960

auccagccgg agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc     1020

gggaugaucg ugaacgacac cggccacgag acggacgaga accgggccaa gguggagauc     1080

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac     1140

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag     1200

cacuggcugg uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc     1260

gacaccggga ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac     1320

gccaagcgcc agaccguggu cgugcugggc ucccaggagg gggccgugca cacggcccug     1380

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1440

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu gaccguggag     1560

guccaguacg ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cgcccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1740

ggcgucgggg agaagaagau cacgcaccac uggcaccgga gcggcagcac ccuggggaag     1800

gccuucucca ccacgcucaa gggcgcccag cgccuggcgg cccuggggga caccgccugg     1860

gacuucggca gcaucggggg cguguucaac uccaucggcc gggccgucca ccagguguuc     1920

gggggcgccu uccgcacccu cuucgggggc augagcugga ucacccaggg gcugaugggc     1980

gcgcugcucc uguggauggg ggugaacgcc cgggaccgcu ccaucgcccu ggccuuccuc     2040

gcgacgggcg gcguccuggu guuccuggcc accaacgugc acgcc                     2085


<210>  9737
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9737
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacc       60

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      360

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      660

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      840

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc     1020

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1140

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1320

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1560

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1620

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1740

acgcaccacu ggcaccggag cggcagcacc cuggggaagg ccuucuccac cacgcucaag     1800

ggcgcccagc gccuggcggc ccugggggac accgccuggg acuucggcag caucgggggc     1860

guguucaacu ccaucggccg ggccguccac cagguguucg ggggcgccuu ccgcacccuc     1920

uucgggggca ugagcuggau cacccagggg cugaugggcg cgcugcuccu guggaugggg     1980

gugaacgccc gggaccgcuc caucgcccug gccuuccucg cgacgggcgg cguccuggug     2040

uuccuggcca ccaacgugca cgcc                                            2064


<210>  9738
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9738
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacc       60

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacacg      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac cacgagcacc ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacgcgcaag      360

cugcagaccc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccacgag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      660

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      840

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg     1020

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug     1140

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc     1320

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1560

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1620

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1740

acccaccacu ggcaccggag cggcagcacc cuggggaagg ccuucuccac cacgcucaag     1800

ggcgcccagc gccuggcggc ccugggggac accgccuggg acuucggcag caucgggggc     1860

guguucaacu ccaucggccg ggccguccac cagguguucg ggggcgccuu ccgcacccuc     1920

uucgggggca ugagcuggau cacccagggg cugaugggcg cgcugcuccu guggaugggg     1980

gugaacgccc gggaccgcuc caucgcccug gccuuccucg cgacgggcgg cguccuggug     2040

uuccuggcca ccaacgugca cgcc                                            2064


<210>  9739
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9739
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcaucacc       60

cgccgggggu ccgccuacua cauguaccug gaccgcagcg acgccggcaa ggccaucucc      120

uucgcgacca cgcugggcgu gaacaagugc cacgugcaga ucauggaccu cgggcacaug      180

ugcgacgcca ccaugagcua cgagugcccg augcuggacg agggcgucga gcccgacgac      240

guggacugcu ggugcaacac caccuccacg uggguggucu acgggaccug ccaccacaag      300

aagggcgagg cccggcgcag ccggcgcgcc gugacccugc ccucccacag cacccggaag      360

cuccagacgc gcucccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      420

guggagaacu ggaucuuccg gaaccccggc uucgcccucg ucgcgguggc caucgccugg      480

cugcuggggu ccagcaccuc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc      540

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      600

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      660

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      720

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      780

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      840

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      900

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg     1020

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug     1080

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac     1140

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1200

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag     1260

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc     1320

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg     1380

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug     1440

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1500

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1560

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1620

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1680

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1740

caccggagcg gcagcacccu ggggaaggcc uucuccacca cgcucaaggg cgcccagcgc     1800

cuggcggccc ugggggacac cgccugggac uucggcagca ucgggggcgu guucaacucc     1860

aucggccggg ccguccacca gguguucggg ggcgccuucc gcacccucuu cgggggcaug     1920

agcuggauca cccaggggcu gaugggcgcg cugcuccugu ggaugggggu gaacgcccgg     1980

gaccgcucca ucgcccuggc cuuccucgcg acgggcggcg uccugguguu ccuggccacc     2040

aacgugcacg cc                                                         2052


<210>  9740
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9740
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgucacc       60

cggcgcggcu ccgccuacua cauguaccuc gaccggaacg acgccgggga ggccaucagc      120

uucccgacca cgcugggcau gaacaagugc uacauccaga ucauggaccu ggggcacaug      180

ugcgacgcga ccauguccua cgagugcccc augcucgacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacg ugggucgugu acggcacgug ccaccacaag      300

aagggggagg cccgccgguc ccgccgggcc gugacccugc ccagccacuc cacccgcaag      360

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      420

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      480

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      540

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      600

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      660

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      720

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      780

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      840

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      900

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      960

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc     1020

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg     1080

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug     1140

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag     1200

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1260

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc     1320

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1380

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1440

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1560

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1620

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1680

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1740

acgcaccacu ggcaccggag cggcagcacc cuggggaagg ccuucuccac cacgcucaag     1800

ggcgcccagc gccuggcggc ccugggggac accgccuggg acuucggcag caucgggggc     1860

guguucaacu ccaucggccg ggccguccac cagguguucg ggggcgccuu ccgcacccuc     1920

uucgggggca ugagcuggau cacccagggg cugaugggcg cgcugcuccu guggaugggg     1980

gugaacgccc gggaccgcuc caucgcccug gccuuccucg cgacgggcgg cguccuggug     2040

uuccuggcca ccaacgugca cgcc                                            2064


<210>  9741
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9741
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacaucg uccuggagca cgggggcugc      660

gugaccguga uggcccagga caagcccacc gucgacaucg agcucgugac cacgaccgug      720

agcaacaugg ccgagguccg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gugugcaagc gcacgcuggu cgaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc      960

cagccggaga accuggagua ccgcaucaug cucuccgugc acggcagcca gcacuccggg     1020

augaucguca acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgucg aguucaagga cgcgcacgcc     1320

aagcgccaga ccgugguggu ccugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguga gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggucac cguggaggug     1560

caguacgccg ggaccgacgg cccgugcaag guccccgccc agauggccgu ggacaugcag     1620

acccugacgc ccguggggcg ccugaucacc gcgaaccccg ucaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gugggggaga agaagaucac gcaccacugg caccggagcg gcagcacccu ggggaaggcc     1800

uucuccacca cgcucaaggg cgcccagcgc cuggcggccc ugggggacac cgccugggac     1860

uucggcagca ucgggggcgu guucaacucc aucggccggg ccguccacca gguguucggg     1920

ggcgccuucc gcacccucuu cgggggcaug agcuggauca cccaggggcu gaugggcgcg     1980

cugcuccugu ggaugggggu gaacgcccgg gaccgcucca ucgcccuggc cuuccucgcg     2040

acgggcggcg uccugguguu ccuggccacc aacgugcacg cc                        2082


<210>  9742
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9742
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacacgug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca cgagcaccug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cgcgcaagcu gcagacccgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccacgagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcaccugg guggacgucg ugcuggagca cgggggcugc      660

gugaccguca uggcccagga caagcccacc guggacaucg agcucgugac gaccaccguc      720

agcaacaugg ccgaggugcg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacgcaguac      840

gucugcaagc gcacccuggu ggaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc      960

cagccggaga accuggagua ccgcaucaug cucuccgucc acggcagcca gcacuccggg     1020

augaucguga acgacacggg ccacgagacc gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga cgggccugga cuucagcgac cuguacuacc ucaccaugaa caacaagcac     1200

uggcuggucc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgugg aguucaagga cgcgcacgcc     1320

aagcgccaga cgguggucgu gcugggcucc caggaggggg ccgugcacac cgcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguca gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acgcuccacg gcaccgugac cguggagguc     1560

caguacgccg ggaccgacgg cccgugcaag gugcccgccc agauggccgu ggacaugcag     1620

acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg ugaucaccga gagcacggag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gucggggaga agaagaucac ccaccacugg caccggagcg gcagcacccu ggggaaggcc     1800

uucuccacca cgcucaaggg cgcccagcgc cuggcggccc ugggggacac cgccugggac     1860

uucggcagca ucgggggcgu guucaacucc aucggccggg ccguccacca gguguucggg     1920

ggcgccuucc gcacccucuu cgggggcaug agcuggauca cccaggggcu gaugggcgcg     1980

cugcuccugu ggaugggggu gaacgcccgg gaccgcucca ucgcccuggc cuuccucgcg     2040

acgggcggcg uccugguguu ccuggccacc aacgugcacg cc                        2082


<210>  9743
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9743
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccaucacccg ccgggggucc gccuacuaca uguaccugga ccgcagcgac      120

gccggcaagg ccaucuccuu cgcgaccacg cugggcguga acaagugcca cgugcagauc      180

auggaccucg ggcacaugug cgacgccacc augagcuacg agugcccgau gcuggacgag      240

ggcgucgagc ccgacgacgu ggacugcugg ugcaacacca ccuccacgug gguggucuac      300

gggaccugcc accacaagaa gggcgaggcc cggcgcagcc ggcgcgccgu gacccugccc      360

ucccacagca cccggaagcu ccagacgcgc ucccagaccu ggcuggagag ccgcgaguac      420

accaagcacc ugaucaaggu ggagaacugg aucuuccgga accccggcuu cgcccucguc      480

gcgguggcca ucgccuggcu gcuggggucc agcaccuccc agaaggugau cuaccucguc      540

augauccugc ugaucgcccc ggccuacagc auccgcugca ucggcguguc caaccgggac      600

uucguggagg ggaugagcgg cgggacgugg gucgacgugg ugcucgagca cggcggcugc      660

gucaccguga uggcgcagga caagcccacc guggacaucg agcuggucac cacgaccgug      720

uccaacaugg ccgagguccg cagcuacugc uacgaggcca gcaucuccga cauggccagc      780

gacucccggu gccccaccca gggggaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaagc gcacgcucgu ggaccggggc ugggggaacg gcugcgggcu guucggcaag      900

ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga agaugaccgg gaaguccauc      960

cagcccgaga accucgagua ccgcaucaug cugagcgugc acggcuccca gcacagcggg     1020

augaucggcu acgagacgga cgaggaccgg gccaaggugg aggucacccc gaacuccccg     1080

cgcgccgagg ccacccuggg cggguucggc agccucgggc uggacugcga gccccggacc     1140

ggccuggacu ucuccgaccu cuacuaccug acgaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccucccg uggcacgcgg gggccgacac cggcaccccg     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gcgccagacc     1320

gucguggugc ugggcagcca ggagggggcg guccacacgg cccuggccgg cgcccucgag     1380

gccgagaugg acggggcgaa gggccggcug uucuccgggc accugaagug ccgccucaag     1440

auggacaagc ugcggcugaa gggcgugagc uacucccucu gcaccgccgc cuucaccuuc     1500

accaaggugc ccgccgagac ccugcacggc acggucaccg uggaggugca guacgccggg     1560

accgacggcc ccugcaagau cccgguccag auggcggugg acaugcagac ccugacgccc     1620

guggggcgcc ucaucaccgc caaccccguc aucaccgaga gcaccgagaa cuccaagaug     1680

augcuggagc uggacccgcc guucggcgac agcuacaucg ugaucggggu gggcgacaag     1740

aagaucacgc accacuggca ccggagcggc agcacccugg ggaaggccuu cuccaccacg     1800

cucaagggcg cccagcgccu ggcggcccug ggggacaccg ccugggacuu cggcagcauc     1860

gggggcgugu ucaacuccau cggccgggcc guccaccagg uguucggggg cgccuuccgc     1920

acccucuucg ggggcaugag cuggaucacc caggggcuga ugggcgcgcu gcuccugugg     1980

auggggguga acgcccggga ccgcuccauc gcccuggccu uccucgcgac gggcggcguc     2040

cugguguucc uggccaccaa cgugcacgcc                                      2070


<210>  9744
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9744
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgucacccg gcgcggcucc gccuacuaca uguaccucga ccggaacgac      120

gccggggagg ccaucagcuu cccgaccacg cugggcauga acaagugcua cauccagauc      180

auggaccugg ggcacaugug cgacgcgacc auguccuacg agugccccau gcucgacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcacgug ggucguguac      300

ggcacgugcc accacaagaa gggggaggcc cgccgguccc gccgggccgu gacccugccc      360

agccacucca cccgcaagcu gcagacgcgg agccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ugauccgggu cgagaacugg aucuuccgca acccgggcuu cgcccuggcc      480

gcggccgcca ucgccuggcu ccuggggagc uccaccagcc agaaggugau cuaccuggug      540

augauccucc ugaucgcccc cgcguacucc auccgcugca ucggcgucag caaccgggac      600

uucguggagg ggauguccgg cggcacgugg guggacgucg ugcuggagca cgggggcugc      660

gugaccguca uggcccagga caagcccacc guggacaucg agcucgugac cacgaccguc      720

agcaacaugg ccgaggugcg cagcuacugc uacgaggccu ccaucagcga cauggccucc      780

gacagccggu gccccaccca gggggaggcg uaccuggaca agcaguccga cacccaguac      840

gucugcaagc gcacgcuggu ggaccggggc ugggggaacg gcugcggccu cuucgggaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga agaugaccgg gaagagcauc      960

cagccggaga accuggagua ccgcaucaug cucuccgucc acggcagcca gcacuccggg     1020

augaucguga acgacaccgg ccacgagacg gacgagaacc gggccaaggu ggagaucacc     1080

cccaacagcc cccgcgcgga ggccacccug ggcggguucg gcucccuggg gcucgacugc     1140

gagccccgga ccggccugga cuucagcgac cuguacuacc ucacgaugaa caacaagcac     1200

uggcuggucc acaaggagug guuccacgac aucccgcugc ccuggcacgc cggcgccgac     1260

accgggaccc cgcacuggaa caacaaggag gcccucgugg aguucaagga cgcgcacgcc     1320

aagcgccaga ccguggucgu gcugggcucc caggaggggg ccgugcacac ggcccuggcc     1380

ggcgcgcugg aggccgagau ggacggggcc aagggccggc ucagcuccgg ccaccugaag     1440

ugccgccuga agauggacaa gcuccggcug aaggggguca gcuacucccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgcggag acccuccacg gcacggugac cguggagguc     1560

caguacgccg ggaccgacgg cccgugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugacgc ccgucgggcg ccugaucacc gcgaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcucga gcuggacccg cccuucggcg acuccuacau cgugaucggc     1740

gucggggaga agaagaucac gcaccacugg caccggagcg gcagcacccu ggggaaggcc     1800

uucuccacca cgcucaaggg cgcccagcgc cuggcggccc ugggggacac cgccugggac     1860

uucggcagca ucgggggcgu guucaacucc aucggccggg ccguccacca gguguucggg     1920

ggcgccuucc gcacccucuu cgggggcaug agcuggauca cccaggggcu gaugggcgcg     1980

cugcuccugu ggaugggggu gaacgcccgg gaccgcucca ucgcccuggc cuuccucgcg     2040

acgggcggcg uccugguguu ccuggccacc aacgugcacg cc                        2082


<210>  9745
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9745
augaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgugacccug       60

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      120

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      180

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      240

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      300

gacuucgugg aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc      360

ugcgugaccg ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc      420

gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc      480

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      540

uacgugugca agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg      600

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      660

auccagccgg agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc      720

gggaugaucg ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc      780

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac      840

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag      900

cacuggcugg ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc      960

gacaccggga ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac     1020

gccaagcgcc agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug     1080

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1140

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc     1200

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag     1260

gugcaguacg ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug     1320

cagacccuga cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc     1380

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1440

ggcguggggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1500

gccuucgagg ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1560

gacuucggca gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1620

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1680

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     1740

gcccugggcg gcguccugau cuuccucucc accgcgguga gcgcc                     1785


<210>  9746
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9746
augaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgugacccug       60

cccagccacu ccacgcgcaa gcugcagacc cggagccaga ccuggcucga gucccgcgag      120

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      180

gccgcggccg ccaucgccug gcuccugggg agcuccacga gccagaaggu gaucuaccug      240

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      300

gacuucgugg aggggauguc cggcggcacc uggguggacg ucgugcugga gcacgggggc      360

ugcgugaccg ucauggccca ggacaagccc accguggaca ucgagcucgu gacgaccacc      420

gucagcaaca uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc      480

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacgcag      540

uacgucugca agcgcacccu gguggaccgg ggcuggggga acggcugcgg ccucuucggg      600

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      660

auccagccgg agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc      720

gggaugaucg ugaacgacac gggccacgag accgacgaga accgggccaa gguggagauc      780

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac      840

ugcgagcccc ggacgggccu ggacuucagc gaccuguacu accucaccau gaacaacaag      900

cacuggcugg uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc      960

gacaccggga ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac     1020

gccaagcgcc agacgguggu cgugcugggc ucccaggagg gggccgugca caccgcccug     1080

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1140

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc     1200

gccgccuuca ccuucaccaa gauccccgcg gagacgcucc acggcaccgu gaccguggag     1260

guccaguacg ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug     1320

cagacgcuga cccccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacg     1380

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1440

ggcgucgggg agaagaagau cacccaccac uggcaccgga gcggcuccac caucgggaag     1500

gccuucgagg ccaccgugcg cggcgccaag cggauggccg ugcuggggga cacggcgugg     1560

gacuucggca gcgucggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1620

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1680

acccugcuga uguggcucgg gcugaacgcc aagaacggcu ccaucagccu gaugugccuc     1740

gcgcugggcg gcgugcugau cuuccucucc accgccguga gcgcc                     1785


<210>  9747
<211>  1773
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9747
augaccagca ucgggaucau cggccugcug cucacgaccg ccauggcggc cgugacccug       60

cccucccaca gcacccggaa gcuccagacg cgcucccaga ccuggcugga gagccgcgag      120

uacaccaagc accugaucaa gguggagaac uggaucuucc ggaaccccgg cuucgcccuc      180

gucgcggugg ccaucgccug gcugcugggg uccagcaccu cccagaaggu gaucuaccuc      240

gucaugaucc ugcugaucgc cccggccuac agcauccgcu gcaucggcgu guccaaccgg      300

gacuucgugg aggggaugag cggcgggacg ugggucgacg uggugcucga gcacggcggc      360

ugcgucaccg ugauggcgca ggacaagccc accguggaca ucgagcuggu caccacgacc      420

guguccaaca uggccgaggu ccgcagcuac ugcuacgagg ccagcaucuc cgacauggcc      480

agcgacuccc ggugccccac ccagggggag gccuaccugg acaagcagag cgacacccag      540

uacgugugca agcgcacgcu cguggaccgg ggcuggggga acggcugcgg gcuguucggc      600

aagggcuccc uggucacgug cgcgaaguuc acgugcagca agaagaugac cgggaagucc      660

auccagcccg agaaccucga guaccgcauc augcugagcg ugcacggcuc ccagcacagc      720

gggaugaucg gcuacgagac ggacgaggac cgggccaagg uggaggucac cccgaacucc      780

ccgcgcgccg aggccacccu gggcggguuc ggcagccucg ggcuggacug cgagccccgg      840

accggccugg acuucuccga ccucuacuac cugacgauga acaacaagca cuggcuggug      900

cacaaggagu gguuccacga caucccccuc ccguggcacg cgggggccga caccggcacc      960

ccgcacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagcgccag     1020

accgucgugg ugcugggcag ccaggagggg gcgguccaca cggcccuggc cggcgcccuc     1080

gaggccgaga uggacggggc gaagggccgg cuguucuccg ggcaccugaa gugccgccuc     1140

aagauggaca agcugcggcu gaagggcgug agcuacuccc ucugcaccgc cgccuucacc     1200

uucaccaagg ugcccgccga gacccugcac ggcacgguca ccguggaggu gcaguacgcc     1260

gggaccgacg gccccugcaa gaucccgguc cagauggcgg uggacaugca gacccugacg     1320

cccguggggc gccucaucac cgccaacccc gucaucaccg agagcaccga gaacuccaag     1380

augaugcugg agcuggaccc gccguucggc gacagcuaca ucgugaucgg ggugggcgac     1440

aagaagauca cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc     1500

accguccgcg gcgcgaagcg gauggccgug cucggggaca ccgccuggga cuucggcagc     1560

gugggcggcg ucuucaacuc ccuggggaag gggauccacc agaucuucgg cgccgccuuc     1620

aagagccugu ucgggggcau guccugguuc agccagaucc ucaucgggac gcugcuggug     1680

uggcucggcc ugaacaccaa gaacggcucc aucagccuga ccugccucgc gcugggcggc     1740

gugaugaucu uccuguccac cgccgucagc gcc                                  1773


<210>  9748
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9748
augaccagcg uggggaucgu gggccuccug cugacgaccg ccauggcggc cgugacccug       60

cccagccacu ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag      120

uacaccaagc accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug      180

gccgcggccg ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug      240

gugaugaucc uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg      300

gacuucgugg aggggauguc cggcggcacg uggguggacg ucgugcugga gcacgggggc      360

ugcgugaccg ucauggccca ggacaagccc accguggaca ucgagcucgu gaccacgacc      420

gucagcaaca uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc      480

uccgacagcc ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag      540

uacgucugca agcgcacgcu gguggaccgg ggcuggggga acggcugcgg ccucuucggg      600

aagggcagcc uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc      660

auccagccgg agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc      720

gggaugaucg ugaacgacac cggccacgag acggacgaga accgggccaa gguggagauc      780

acccccaaca gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac      840

ugcgagcccc ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag      900

cacuggcugg uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc      960

gacaccggga ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac     1020

gccaagcgcc agaccguggu cgugcugggc ucccaggagg gggccgugca cacggcccug     1080

gccggcgcgc uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug     1140

aagugccgcc ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc     1200

gccgccuuca ccuucaccaa gauccccgcg gagacccucc acggcacggu gaccguggag     1260

guccaguacg ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug     1320

cagacccuga cgcccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacc     1380

gagaacagca agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc     1440

ggcgucgggg agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1500

gccuucgagg ccaccgugcg cggcgccaag cggauggccg ugcuggggga caccgcgugg     1560

gacuucggca gcgucggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc     1620

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc     1680

acgcugcuga uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc     1740

gcccugggcg gcgugcugau cuuccucucc accgcgguga gcgcc                     1785


<210>  9749
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9749
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      120

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      180

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      240

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      300

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      360

guggacaucg uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc      420

gucgacaucg agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc      480

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      540

uaccuggaca agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc      600

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      660

ugcuccaaga agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug      720

cucuccgugc acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg      780

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug      840

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac      900

cuguacuacc ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      960

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1020

gcccucgucg aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc     1080

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1140

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1200

aaggggguga gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1260

acccuccacg gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag     1320

guccccgccc agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc     1380

gcgaaccccg ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1440

cccuucggcg acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg     1500

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg     1560

auggccgugc ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc     1620

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1680

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     1740

aacggcucca ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc     1800

gcggugagcg cc                                                         1812


<210>  9750
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9750
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgccgu gacccugccc agccacucca cgcgcaagcu gcagacccgg      120

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      180

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      240

uccacgagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      300

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg      360

guggacgucg ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc      420

guggacaucg agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc      480

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      540

uaccuggaca agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc      600

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      660

ugcuccaaga agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug      720

cucuccgucc acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc      780

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug      840

ggcggguucg gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac      900

cuguacuacc ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac      960

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1020

gcccucgugg aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc     1080

caggaggggg ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1140

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1200

aaggggguca gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1260

acgcuccacg gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag     1320

gugcccgccc agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc     1380

gcgaaccccg ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg     1440

cccuucggcg acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg     1500

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg     1560

auggccgugc ugggggacac ggcgugggac uucggcagcg ucggcggggc ccucaacucc     1620

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1680

uccugguuca gccagauccu caucggcacc cugcugaugu ggcucgggcu gaacgccaag     1740

aacggcucca ucagccugau gugccucgcg cugggcggcg ugcugaucuu ccucuccacc     1800

gccgugagcg cc                                                         1812


<210>  9751
<211>  1800
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9751
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgccgu gacccugccc ucccacagca cccggaagcu ccagacgcgc      120

ucccagaccu ggcuggagag ccgcgaguac accaagcacc ugaucaaggu ggagaacugg      180

aucuuccgga accccggcuu cgcccucguc gcgguggcca ucgccuggcu gcuggggucc      240

agcaccuccc agaaggugau cuaccucguc augauccugc ugaucgcccc ggccuacagc      300

auccgcugca ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg      360

gucgacgugg ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc      420

guggacaucg agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc      480

uacgaggcca gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc      540

uaccuggaca agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc      600

ugggggaacg gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg      660

ugcagcaaga agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug      720

cugagcgugc acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg      780

gccaaggugg aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc      840

agccucgggc uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug      900

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg      960

uggcacgcgg gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag     1020

uucaaggacg cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg     1080

guccacacgg cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug     1140

uucuccgggc accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc     1200

uacucccucu gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc     1260

acggucaccg uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag     1320

auggcggugg acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc     1380

aucaccgaga gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac     1440

agcuacaucg ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc     1500

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc     1560

ggggacaccg ccugggacuu cggcagcgug ggcggcgucu ucaacucccu ggggaagggg     1620

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1680

cagauccuca ucgggacgcu gcuggugugg cucggccuga acaccaagaa cggcuccauc     1740

agccugaccu gccucgcgcu gggcggcgug augaucuucc uguccaccgc cgucagcgcc     1800


<210>  9752
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9752
auggccauca gcggcgugcc cguccugggg uucuucauca ucgccgugcu cauguccgcg       60

caggagagcu gggccgccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg      120

agccagaccu ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg      180

aucuuccgca acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc      240

uccaccagcc agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc      300

auccgcugca ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg      360

guggacgucg ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc      420

guggacaucg agcucgugac cacgaccguc agcaacaugg ccgaggugcg cagcuacugc      480

uacgaggccu ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg      540

uaccuggaca agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc      600

ugggggaacg gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc      660

ugcuccaaga agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug      720

cucuccgucc acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacg      780

gacgagaacc gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug      840

ggcggguucg gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac      900

cuguacuacc ucacgaugaa caacaagcac uggcuggucc acaaggagug guuccacgac      960

aucccgcugc ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag     1020

gcccucgugg aguucaagga cgcgcacgcc aagcgccaga ccguggucgu gcugggcucc     1080

caggaggggg ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc     1140

aagggccggc ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug     1200

aaggggguca gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag     1260

acccuccacg gcacggugac cguggagguc caguacgccg ggaccgacgg cccgugcaag     1320

gugcccgccc agauggccgu ggacaugcag acccugacgc ccgucgggcg ccugaucacc     1380

gcgaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg     1440

cccuucggcg acuccuacau cgugaucggc gucggggaga agaagaucac gcaccacugg     1500

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg     1560

auggccgugc ugggggacac cgcgugggac uucggcagcg ucggcggggc ccucaacucc     1620

cugggcaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1680

uccugguuca gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag     1740

aacggcucca ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc     1800

gcggugagcg cc                                                         1812


<210>  9753
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9753
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgccgug       60

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      120

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      180

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      240

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      300

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacaucgu ccuggagcac      360

gggggcugcg ugaccgugau ggcccaggac aagcccaccg ucgacaucga gcucgugacc      420

acgaccguga gcaacauggc cgagguccgc agcuacugcu acgaggccuc caucagcgac      480

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      540

acccaguacg ugugcaagcg cacgcugguc gaccggggcu gggggaacgg cugcggccuc      600

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      660

aagagcaucc agccggagaa ccuggaguac cgcaucaugc ucuccgugca cggcagccag      720

cacuccggga ugaucgucaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug      780

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg      840

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac      900

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc      960

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgucga guucaaggac     1020

gcgcacgcca agcgccagac cguggugguc cugggcuccc aggagggggc cgugcacacg     1080

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1140

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggugag cuacucccug     1200

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggucacc     1260

guggaggugc aguacgccgg gaccgacggc ccgugcaagg uccccgccca gauggccgug     1320

gacaugcaga cccugacgcc cguggggcgc cugaucaccg cgaaccccgu caucaccgag     1380

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1440

gugaucggcg ugggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1500

gggaaggccu ucgaggccac cguccgcggc gccaagcgga uggccgugcu gggggacacc     1560

gcgugggacu ucggcagcgu gggcggggcc cucaacuccc ugggcaaggg gauccaccag     1620

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1680

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     1740

ugccucgccc ugggcggcgu ccugaucuuc cucuccaccg cggugagcgc c              1791


<210>  9754
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9754
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgccgug       60

acccugccca gccacuccac gcgcaagcug cagacccgga gccagaccug gcucgagucc      120

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      180

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccacgagcca gaaggugauc      240

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      300

aaccgggacu ucguggaggg gauguccggc ggcaccuggg uggacgucgu gcuggagcac      360

gggggcugcg ugaccgucau ggcccaggac aagcccaccg uggacaucga gcucgugacg      420

accaccguca gcaacauggc cgaggugcgc agcuacugcu acgaggccuc caucagcgac      480

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      540

acgcaguacg ucugcaagcg cacccuggug gaccggggcu gggggaacgg cugcggccuc      600

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      660

aagagcaucc agccggagaa ccuggaguac cgcaucaugc ucuccgucca cggcagccag      720

cacuccggga ugaucgugaa cgacacgggc cacgagaccg acgagaaccg ggccaaggug      780

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg      840

cucgacugcg agccccggac gggccuggac uucagcgacc uguacuaccu caccaugaac      900

aacaagcacu ggcuggucca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc      960

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgugga guucaaggac     1020

gcgcacgcca agcgccagac gguggucgug cugggcuccc aggagggggc cgugcacacc     1080

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1140

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggucag cuacucccug     1200

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cgcuccacgg caccgugacc     1260

guggaggucc aguacgccgg gaccgacggc ccgugcaagg ugcccgccca gauggccgug     1320

gacaugcaga cgcugacccc cgucgggcgc cugaucaccg cgaaccccgu gaucaccgag     1380

agcacggaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1440

gugaucggcg ucggggagaa gaagaucacc caccacuggc accggagcgg cuccaccauc     1500

gggaaggccu ucgaggccac cgugcgcggc gccaagcgga uggccgugcu gggggacacg     1560

gcgugggacu ucggcagcgu cggcggggcc cucaacuccc ugggcaaggg gauccaccag     1620

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1680

aucggcaccc ugcugaugug gcucgggcug aacgccaaga acggcuccau cagccugaug     1740

ugccucgcgc ugggcggcgu gcugaucuuc cucuccaccg ccgugagcgc c              1791


<210>  9755
<211>  1779
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9755
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgccgug       60

acccugcccu cccacagcac ccggaagcuc cagacgcgcu cccagaccug gcuggagagc      120

cgcgaguaca ccaagcaccu gaucaaggug gagaacugga ucuuccggaa ccccggcuuc      180

gcccucgucg cgguggccau cgccuggcug cuggggucca gcaccuccca gaaggugauc      240

uaccucguca ugauccugcu gaucgccccg gccuacagca uccgcugcau cggcgugucc      300

aaccgggacu ucguggaggg gaugagcggc gggacguggg ucgacguggu gcucgagcac      360

ggcggcugcg ucaccgugau ggcgcaggac aagcccaccg uggacaucga gcuggucacc      420

acgaccgugu ccaacauggc cgagguccgc agcuacugcu acgaggccag caucuccgac      480

auggccagcg acucccggug ccccacccag ggggaggccu accuggacaa gcagagcgac      540

acccaguacg ugugcaagcg cacgcucgug gaccggggcu gggggaacgg cugcgggcug      600

uucggcaagg gcucccuggu cacgugcgcg aaguucacgu gcagcaagaa gaugaccggg      660

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ugagcgugca cggcucccag      720

cacagcggga ugaucggcua cgagacggac gaggaccggg ccaaggugga ggucaccccg      780

aacuccccgc gcgccgaggc cacccugggc ggguucggca gccucgggcu ggacugcgag      840

ccccggaccg gccuggacuu cuccgaccuc uacuaccuga cgaugaacaa caagcacugg      900

cuggugcaca aggagugguu ccacgacauc ccccucccgu ggcacgcggg ggccgacacc      960

ggcaccccgc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1020

cgccagaccg ucguggugcu gggcagccag gagggggcgg uccacacggc ccuggccggc     1080

gcccucgagg ccgagaugga cggggcgaag ggccggcugu ucuccgggca ccugaagugc     1140

cgccucaaga uggacaagcu gcggcugaag ggcgugagcu acucccucug caccgccgcc     1200

uucaccuuca ccaaggugcc cgccgagacc cugcacggca cggucaccgu ggaggugcag     1260

uacgccggga ccgacggccc cugcaagauc ccgguccaga uggcggugga caugcagacc     1320

cugacgcccg uggggcgccu caucaccgcc aaccccguca ucaccgagag caccgagaac     1380

uccaagauga ugcuggagcu ggacccgccg uucggcgaca gcuacaucgu gaucggggug     1440

ggcgacaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1500

gaggccaccg uccgcggcgc gaagcggaug gccgugcucg gggacaccgc cugggacuuc     1560

ggcagcgugg gcggcgucuu caacucccug gggaagggga uccaccagau cuucggcgcc     1620

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cgggacgcug     1680

cugguguggc ucggccugaa caccaagaac ggcuccauca gccugaccug ccucgcgcug     1740

ggcggcguga ugaucuuccu guccaccgcc gucagcgcc                            1779


<210>  9756
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9756
auggacugga ccuggauccu guuccucgug gccgccgcga cccgcgucca cagcgccgug       60

acccugccca gccacuccac ccgcaagcug cagacgcgga gccagaccug gcucgagucc      120

cgcgaguaca ccaagcaccu gauccggguc gagaacugga ucuuccgcaa cccgggcuuc      180

gcccuggccg cggccgccau cgccuggcuc cuggggagcu ccaccagcca gaaggugauc      240

uaccugguga ugauccuccu gaucgccccc gcguacucca uccgcugcau cggcgucagc      300

aaccgggacu ucguggaggg gauguccggc ggcacguggg uggacgucgu gcuggagcac      360

gggggcugcg ugaccgucau ggcccaggac aagcccaccg uggacaucga gcucgugacc      420

acgaccguca gcaacauggc cgaggugcgc agcuacugcu acgaggccuc caucagcgac      480

auggccuccg acagccggug ccccacccag ggggaggcgu accuggacaa gcaguccgac      540

acccaguacg ucugcaagcg cacgcuggug gaccggggcu gggggaacgg cugcggccuc      600

uucgggaagg gcagccuggu gaccugcgcc aaguucgccu gcuccaagaa gaugaccggg      660

aagagcaucc agccggagaa ccuggaguac cgcaucaugc ucuccgucca cggcagccag      720

cacuccggga ugaucgugaa cgacaccggc cacgagacgg acgagaaccg ggccaaggug      780

gagaucaccc ccaacagccc ccgcgcggag gccacccugg gcggguucgg cucccugggg      840

cucgacugcg agccccggac cggccuggac uucagcgacc uguacuaccu cacgaugaac      900

aacaagcacu ggcuggucca caaggagugg uuccacgaca ucccgcugcc cuggcacgcc      960

ggcgccgaca ccgggacccc gcacuggaac aacaaggagg cccucgugga guucaaggac     1020

gcgcacgcca agcgccagac cguggucgug cugggcuccc aggagggggc cgugcacacg     1080

gcccuggccg gcgcgcugga ggccgagaug gacggggcca agggccggcu cagcuccggc     1140

caccugaagu gccgccugaa gauggacaag cuccggcuga agggggucag cuacucccug     1200

ugcaccgccg ccuucaccuu caccaagauc cccgcggaga cccuccacgg cacggugacc     1260

guggaggucc aguacgccgg gaccgacggc ccgugcaagg ugcccgccca gauggccgug     1320

gacaugcaga cccugacgcc cgucgggcgc cugaucaccg cgaaccccgu gaucaccgag     1380

agcaccgaga acagcaagau gaugcucgag cuggacccgc ccuucggcga cuccuacauc     1440

gugaucggcg ucggggagaa gaagaucacg caccacuggc accggagcgg cuccaccauc     1500

gggaaggccu ucgaggccac cgugcgcggc gccaagcgga uggccgugcu gggggacacc     1560

gcgugggacu ucggcagcgu cggcggggcc cucaacuccc ugggcaaggg gauccaccag     1620

aucuucggcg ccgccuucaa gagccuguuc gggggcaugu ccugguucag ccagauccuc     1680

aucggcacgc ugcugaugug gcucgggcug aacaccaaga acggcuccau cagccugaug     1740

ugccucgccc ugggcggcgu gcugaucuuc cucuccaccg cggugagcgc c              1791


<210>  9757
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9757
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      120

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      180

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      240

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      300

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      360

gacaucgucc uggagcacgg gggcugcgug accgugaugg cccaggacaa gcccaccguc      420

gacaucgagc ucgugaccac gaccgugagc aacauggccg agguccgcag cuacugcuac      480

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      540

cuggacaagc aguccgacac ccaguacgug ugcaagcgca cgcuggucga ccggggcugg      600

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      660

uccaagaaga ugaccgggaa gagcauccag ccggagaacc uggaguaccg caucaugcuc      720

uccgugcacg gcagccagca cuccgggaug aucgucaacg acaccggcca cgagacggac      780

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc      840

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug      900

uacuaccuca cgaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc      960

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1020

cucgucgagu ucaaggacgc gcacgccaag cgccagaccg uggugguccu gggcucccag     1080

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1140

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1200

ggggugagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1260

cuccacggca cggucaccgu ggaggugcag uacgccggga ccgacggccc gugcaagguc     1320

cccgcccaga uggccgugga caugcagacc cugacgcccg uggggcgccu gaucaccgcg     1380

aaccccguca ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1440

uucggcgacu ccuacaucgu gaucggcgug ggggagaaga agaucacgca ccacuggcac     1500

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg uccgcggcgc caagcggaug     1560

gccgugcugg gggacaccgc gugggacuuc ggcagcgugg gcggggcccu caacucccug     1620

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1680

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     1740

ggcuccauca gccugaugug ccucgcccug ggcggcgucc ugaucuuccu cuccaccgcg     1800

gugagcgcc                                                             1809


<210>  9758
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9758
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgccgugac ccugcccagc cacuccacgc gcaagcugca gacccggagc      120

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      180

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      240

acgagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      300

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg caccugggug      360

gacgucgugc uggagcacgg gggcugcgug accgucaugg cccaggacaa gcccaccgug      420

gacaucgagc ucgugacgac caccgucagc aacauggccg aggugcgcag cuacugcuac      480

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      540

cuggacaagc aguccgacac gcaguacguc ugcaagcgca cccuggugga ccggggcugg      600

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      660

uccaagaaga ugaccgggaa gagcauccag ccggagaacc uggaguaccg caucaugcuc      720

uccguccacg gcagccagca cuccgggaug aucgugaacg acacgggcca cgagaccgac      780

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc      840

ggguucggcu cccuggggcu cgacugcgag ccccggacgg gccuggacuu cagcgaccug      900

uacuaccuca ccaugaacaa caagcacugg cugguccaca aggagugguu ccacgacauc      960

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1020

cucguggagu ucaaggacgc gcacgccaag cgccagacgg uggucgugcu gggcucccag     1080

gagggggccg ugcacaccgc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1140

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1200

ggggucagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacg     1260

cuccacggca ccgugaccgu ggagguccag uacgccggga ccgacggccc gugcaaggug     1320

cccgcccaga uggccgugga caugcagacg cugacccccg ucgggcgccu gaucaccgcg     1380

aaccccguga ucaccgagag cacggagaac agcaagauga ugcucgagcu ggacccgccc     1440

uucggcgacu ccuacaucgu gaucggcguc ggggagaaga agaucaccca ccacuggcac     1500

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg ugcgcggcgc caagcggaug     1560

gccgugcugg gggacacggc gugggacuuc ggcagcgucg gcggggcccu caacucccug     1620

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1680

ugguucagcc agauccucau cggcacccug cugauguggc ucgggcugaa cgccaagaac     1740

ggcuccauca gccugaugug ccucgcgcug ggcggcgugc ugaucuuccu cuccaccgcc     1800

gugagcgcc                                                             1809


<210>  9759
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9759
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgccgugac ccugcccucc cacagcaccc ggaagcucca gacgcgcucc      120

cagaccuggc uggagagccg cgaguacacc aagcaccuga ucaaggugga gaacuggauc      180

uuccggaacc ccggcuucgc ccucgucgcg guggccaucg ccuggcugcu gggguccagc      240

accucccaga aggugaucua ccucgucaug auccugcuga ucgccccggc cuacagcauc      300

cgcugcaucg gcguguccaa ccgggacuuc guggagggga ugagcggcgg gacguggguc      360

gacguggugc ucgagcacgg cggcugcguc accgugaugg cgcaggacaa gcccaccgug      420

gacaucgagc uggucaccac gaccgugucc aacauggccg agguccgcag cuacugcuac      480

gaggccagca ucuccgacau ggccagcgac ucccggugcc ccacccaggg ggaggccuac      540

cuggacaagc agagcgacac ccaguacgug ugcaagcgca cgcucgugga ccggggcugg      600

gggaacggcu gcgggcuguu cggcaagggc ucccugguca cgugcgcgaa guucacgugc      660

agcaagaaga ugaccgggaa guccauccag cccgagaacc ucgaguaccg caucaugcug      720

agcgugcacg gcucccagca cagcgggaug aucggcuacg agacggacga ggaccgggcc      780

aagguggagg ucaccccgaa cuccccgcgc gccgaggcca cccugggcgg guucggcagc      840

cucgggcugg acugcgagcc ccggaccggc cuggacuucu ccgaccucua cuaccugacg      900

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccucccgugg      960

cacgcggggg ccgacaccgg caccccgcac uggaacaaca aggaggcccu gguggaguuc     1020

aaggacgccc acgccaagcg ccagaccguc guggugcugg gcagccagga gggggcgguc     1080

cacacggccc uggccggcgc ccucgaggcc gagauggacg gggcgaaggg ccggcuguuc     1140

uccgggcacc ugaagugccg ccucaagaug gacaagcugc ggcugaaggg cgugagcuac     1200

ucccucugca ccgccgccuu caccuucacc aaggugcccg ccgagacccu gcacggcacg     1260

gucaccgugg aggugcagua cgccgggacc gacggccccu gcaagauccc gguccagaug     1320

gcgguggaca ugcagacccu gacgcccgug gggcgccuca ucaccgccaa ccccgucauc     1380

accgagagca ccgagaacuc caagaugaug cuggagcugg acccgccguu cggcgacagc     1440

uacaucguga ucgggguggg cgacaagaag aucacgcacc acuggcaccg gagcggcucc     1500

accaucggga aggccuucga ggccaccguc cgcggcgcga agcggauggc cgugcucggg     1560

gacaccgccu gggacuucgg cagcgugggc ggcgucuuca acucccuggg gaaggggauc     1620

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1680

auccucaucg ggacgcugcu gguguggcuc ggccugaaca ccaagaacgg cuccaucagc     1740

cugaccugcc ucgcgcuggg cggcgugaug aucuuccugu ccaccgccgu cagcgcc        1797


<210>  9760
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9760
augggcaagc gcagcgccgg guccaucaug uggcuggcca gccucgcggu ggucaucgcc       60

ugcgccggcg ccgccgugac ccugcccagc cacuccaccc gcaagcugca gacgcggagc      120

cagaccuggc ucgagucccg cgaguacacc aagcaccuga uccgggucga gaacuggauc      180

uuccgcaacc cgggcuucgc ccuggccgcg gccgccaucg ccuggcuccu ggggagcucc      240

accagccaga aggugaucua ccuggugaug auccuccuga ucgcccccgc guacuccauc      300

cgcugcaucg gcgucagcaa ccgggacuuc guggagggga uguccggcgg cacgugggug      360

gacgucgugc uggagcacgg gggcugcgug accgucaugg cccaggacaa gcccaccgug      420

gacaucgagc ucgugaccac gaccgucagc aacauggccg aggugcgcag cuacugcuac      480

gaggccucca ucagcgacau ggccuccgac agccggugcc ccacccaggg ggaggcguac      540

cuggacaagc aguccgacac ccaguacguc ugcaagcgca cgcuggugga ccggggcugg      600

gggaacggcu gcggccucuu cgggaagggc agccugguga ccugcgccaa guucgccugc      660

uccaagaaga ugaccgggaa gagcauccag ccggagaacc uggaguaccg caucaugcuc      720

uccguccacg gcagccagca cuccgggaug aucgugaacg acaccggcca cgagacggac      780

gagaaccggg ccaaggugga gaucaccccc aacagccccc gcgcggaggc cacccugggc      840

ggguucggcu cccuggggcu cgacugcgag ccccggaccg gccuggacuu cagcgaccug      900

uacuaccuca cgaugaacaa caagcacugg cugguccaca aggagugguu ccacgacauc      960

ccgcugcccu ggcacgccgg cgccgacacc gggaccccgc acuggaacaa caaggaggcc     1020

cucguggagu ucaaggacgc gcacgccaag cgccagaccg uggucgugcu gggcucccag     1080

gagggggccg ugcacacggc ccuggccggc gcgcuggagg ccgagaugga cggggccaag     1140

ggccggcuca gcuccggcca ccugaagugc cgccugaaga uggacaagcu ccggcugaag     1200

ggggucagcu acucccugug caccgccgcc uucaccuuca ccaagauccc cgcggagacc     1260

cuccacggca cggugaccgu ggagguccag uacgccggga ccgacggccc gugcaaggug     1320

cccgcccaga uggccgugga caugcagacc cugacgcccg ucgggcgccu gaucaccgcg     1380

aaccccguga ucaccgagag caccgagaac agcaagauga ugcucgagcu ggacccgccc     1440

uucggcgacu ccuacaucgu gaucggcguc ggggagaaga agaucacgca ccacuggcac     1500

cggagcggcu ccaccaucgg gaaggccuuc gaggccaccg ugcgcggcgc caagcggaug     1560

gccgugcugg gggacaccgc gugggacuuc ggcagcgucg gcggggcccu caacucccug     1620

ggcaagggga uccaccagau cuucggcgcc gccuucaaga gccuguucgg gggcaugucc     1680

ugguucagcc agauccucau cggcacgcug cugauguggc ucgggcugaa caccaagaac     1740

ggcuccauca gccugaugug ccucgcccug ggcggcgugc ugaucuuccu cuccaccgcg     1800

gugagcgcc                                                             1809


<210>  9761
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9761
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgcc                     2085


<210>  9762
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9762
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacac cugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacgcc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgcc                     2085


<210>  9763
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9763
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccaucac ccgccgcggc uccgccuacu acauguaccu cgaccgcucc      120

gacgccggca aggccaucuc cuucgccacc acccucggcg ucaacaagug ccacguccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucaucaa ggucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gucgccgucg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc accugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg gcuacgagac cgacgaggac cgcgccaagg ucgaggucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuucuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaagg uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gauccccguc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgac     1740

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1800

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1860

gucggcggcg ucuucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucguc     1980

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ccugccucgc ccucggcggc     2040

gucaugaucu uccucuccac cgccgucucc gcc                                  2073


<210>  9764
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9764
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     2040

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgcc                     2085


<210>  9765
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9765
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1800

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1860

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1920

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1980

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2040

uuccucucca ccgccgucuc cgcc                                            2064


<210>  9766
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9766
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacacc      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1800

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1860

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1920

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1980

cucaacgcca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2040

uuccucucca ccgccgucuc cgcc                                            2064


<210>  9767
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9767
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccaucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      120

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc     1020

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1080

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1140

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1200

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1260

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1320

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1380

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1440

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1500

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1560

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1620

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1680

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1740

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1800

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1860

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1920

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     1980

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     2040

gccgucuccg cc                                                         2052


<210>  9768
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9768
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1800

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1860

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1920

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1980

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     2040

uuccucucca ccgccgucuc cgcc                                            2064


<210>  9769
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9769
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg cc                        2082


<210>  9770
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9770
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaccug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacgucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacgccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg cc                        2082


<210>  9771
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9771
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccaucacccg ccgcggcucc gccuacuaca uguaccucga ccgcuccgac      120

gccggcaagg ccaucuccuu cgccaccacc cucggcguca acaagugcca cguccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucaucaaggu cgagaacugg aucuuccgca accccggcuu cgcccucguc      480

gccgucgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacgucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucacc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucggcu acgagaccga cgaggaccgc gccaaggucg aggucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uucuccggcc accucaagug ccgccucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

accaaggucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

accgacggcc ccugcaagau ccccguccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgacaag     1740

aagaucaccc accacuggca ccgcuccggc uccaccaucg gcaaggccuu cgaggccacc     1800

guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg ccugggacuu cggcuccguc     1860

ggcggcgucu ucaacucccu cggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

ucccucuucg gcggcauguc cugguucucc cagauccuca ucggcacccu ccucgucugg     1980

cucggccuca acaccaagaa cggcuccauc ucccucaccu gccucgcccu cggcggcguc     2040

augaucuucc ucuccaccgc cgucuccgcc                                      2070


<210>  9772
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9772
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacgucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc     1800

uucgaggcca ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac     1860

uucggcuccg ucggcggcgc ccucaacucc cucggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc     1980

cuccucaugu ggcucggccu caacaccaag aacggcucca ucucccucau gugccucgcc     2040

cucggcggcg uccucaucuu ccucuccacc gccgucuccg cc                        2082


<210>  9773
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9773
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac ccucggcaag     1800

gccuucucca ccacccucaa gggcgcccag cgccucgccg cccucggcga caccgccugg     1860

gacuucggcu ccaucggcgg cgucuucaac uccaucggcc gcgccgucca ccaggucuuc     1920

ggcggcgccu uccgcacccu cuucggcggc auguccugga ucacccaggg ccucaugggc     1980

gcccuccucc ucuggauggg cgucaacgcc cgcgaccgcu ccaucgcccu cgccuuccuc     2040

gccaccggcg gcguccucgu cuuccucgcc accaacgucc acgcc                     2085


<210>  9774
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9774
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacac cugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac ccucggcaag     1800

gccuucucca ccacccucaa gggcgcccag cgccucgccg cccucggcga caccgccugg     1860

gacuucggcu ccaucggcgg cgucuucaac uccaucggcc gcgccgucca ccaggucuuc     1920

ggcggcgccu uccgcacccu cuucggcggc auguccugga ucacccaggg ccucaugggc     1980

gcccuccucc ucuggauggg cgucaacgcc cgcgaccgcu ccaucgcccu cgccuuccuc     2040

gccaccggcg gcguccucgu cuuccucgcc accaacgucc acgcc                     2085


<210>  9775
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9775
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccaucac ccgccgcggc uccgccuacu acauguaccu cgaccgcucc      120

gacgccggca aggccaucuc cuucgccacc acccucggcg ucaacaagug ccacguccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucaucaa ggucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gucgccgucg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc accugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg gcuacgagac cgacgaggac cgcgccaagg ucgaggucac ccccaacucc     1080

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc     1140

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc     1200

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1320

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1380

gaggccgaga uggacggcgc caagggccgc cucuucuccg gccaccucaa gugccgccuc     1440

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1500

uucaccaagg uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1560

ggcaccgacg gccccugcaa gauccccguc cagauggccg ucgacaugca gacccucacc     1620

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1680

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgac     1740

aagaagauca cccaccacug gcaccgcucc ggcuccaccc ucggcaaggc cuucuccacc     1800

acccucaagg gcgcccagcg ccucgccgcc cucggcgaca ccgccuggga cuucggcucc     1860

aucggcggcg ucuucaacuc caucggccgc gccguccacc aggucuucgg cggcgccuuc     1920

cgcacccucu ucggcggcau guccuggauc acccagggcc ucaugggcgc ccuccuccuc     1980

uggaugggcg ucaacgcccg cgaccgcucc aucgcccucg ccuuccucgc caccggcggc     2040

guccucgucu uccucgccac caacguccac gcc                                  2073


<210>  9776
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9776
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac      120

gacgccggcg aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag      180

aucauggacc ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac      240

gagggcgucg agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc      300

uacggcaccu gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc      360

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      420

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      480

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      540

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      600

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      660

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      720

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      780

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      840

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      900

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      960

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc     1020

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac ccucggcaag     1800

gccuucucca ccacccucaa gggcgcccag cgccucgccg cccucggcga caccgccugg     1860

gacuucggcu ccaucggcgg cgucuucaac uccaucggcc gcgccgucca ccaggucuuc     1920

ggcggcgccu uccgcacccu cuucggcggc auguccugga ucacccaggg ccucaugggc     1980

gcccuccucc ucuggauggg cgucaacgcc cgcgaccgcu ccaucgcccu cgccuuccuc     2040

gccaccggcg gcguccucgu cuuccucgcc accaacgucc acgcc                     2085


<210>  9777
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9777
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc cucggcaagg ccuucuccac cacccucaag     1800

ggcgcccagc gccucgccgc ccucggcgac accgccuggg acuucggcuc caucggcggc     1860

gucuucaacu ccaucggccg cgccguccac caggucuucg gcggcgccuu ccgcacccuc     1920

uucggcggca uguccuggau cacccagggc cucaugggcg cccuccuccu cuggaugggc     1980

gucaacgccc gcgaccgcuc caucgcccuc gccuuccucg ccaccggcgg cguccucguc     2040

uuccucgcca ccaacgucca cgcc                                            2064


<210>  9778
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9778
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacacc      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc cucggcaagg ccuucuccac cacccucaag     1800

ggcgcccagc gccucgccgc ccucggcgac accgccuggg acuucggcuc caucggcggc     1860

gucuucaacu ccaucggccg cgccguccac caggucuucg gcggcgccuu ccgcacccuc     1920

uucggcggca uguccuggau cacccagggc cucaugggcg cccuccuccu cuggaugggc     1980

gucaacgccc gcgaccgcuc caucgcccuc gccuuccucg ccaccggcgg cguccucguc     2040

uuccucgcca ccaacgucca cgcc                                            2064


<210>  9779
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9779
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccaucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcuccg acgccggcaa ggccaucucc      120

uucgccacca cccucggcgu caacaagugc cacguccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc     1020

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc     1080

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac     1140

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac     1200

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1260

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1320

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1380

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1440

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1500

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1560

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1620

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1680

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1740

caccgcuccg gcuccacccu cggcaaggcc uucuccacca cccucaaggg cgcccagcgc     1800

cucgccgccc ucggcgacac cgccugggac uucggcucca ucggcggcgu cuucaacucc     1860

aucggccgcg ccguccacca ggucuucggc ggcgccuucc gcacccucuu cggcggcaug     1920

uccuggauca cccagggccu caugggcgcc cuccuccucu ggaugggcgu caacgcccgc     1980

gaccgcucca ucgcccucgc cuuccucgcc accggcggcg uccucgucuu ccucgccacc     2040

aacguccacg cc                                                         2052


<210>  9780
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9780
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgucacc       60

cgccgcggcu ccgccuacua cauguaccuc gaccgcaacg acgccggcga ggccaucucc      120

uuccccacca cccucggcau gaacaagugc uacauccaga ucauggaccu cggccacaug      180

ugcgacgcca ccauguccua cgagugcccc augcucgacg agggcgucga gcccgacgac      240

gucgacugcu ggugcaacac caccuccacc ugggucgucu acggcaccug ccaccacaag      300

aagggcgagg cccgccgcuc ccgccgcgcc gucacccucc ccucccacuc cacccgcaag      360

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      420

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      480

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      540

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      600

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      660

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      720

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      780

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      840

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      900

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      960

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc     1020

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc     1080

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc     1140

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag     1200

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1320

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1380

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1440

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1560

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1620

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1680

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1740

acccaccacu ggcaccgcuc cggcuccacc cucggcaagg ccuucuccac cacccucaag     1800

ggcgcccagc gccucgccgc ccucggcgac accgccuggg acuucggcuc caucggcggc     1860

gucuucaacu ccaucggccg cgccguccac caggucuucg gcggcgccuu ccgcacccuc     1920

uucggcggca uguccuggau cacccagggc cucaugggcg cccuccuccu cuggaugggc     1980

gucaacgccc gcgaccgcuc caucgcccuc gccuuccucg ccaccggcgg cguccucguc     2040

uuccucgcca ccaacgucca cgcc                                            2064


<210>  9781
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9781
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacaucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccacccu cggcaaggcc     1800

uucuccacca cccucaaggg cgcccagcgc cucgccgccc ucggcgacac cgccugggac     1860

uucggcucca ucggcggcgu cuucaacucc aucggccgcg ccguccacca ggucuucggc     1920

ggcgccuucc gcacccucuu cggcggcaug uccuggauca cccagggccu caugggcgcc     1980

cuccuccucu ggaugggcgu caacgcccgc gaccgcucca ucgcccucgc cuuccucgcc     2040

accggcggcg uccucgucuu ccucgccacc aacguccacg cc                        2082


<210>  9782
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9782
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaccug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacgucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccacccu cggcaaggcc     1800

uucuccacca cccucaaggg cgcccagcgc cucgccgccc ucggcgacac cgccugggac     1860

uucggcucca ucggcggcgu cuucaacucc aucggccgcg ccguccacca ggucuucggc     1920

ggcgccuucc gcacccucuu cggcggcaug uccuggauca cccagggccu caugggcgcc     1980

cuccuccucu ggaugggcgu caacgcccgc gaccgcucca ucgcccucgc cuuccucgcc     2040

accggcggcg uccucgucuu ccucgccacc aacguccacg cc                        2082


<210>  9783
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9783
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccaucacccg ccgcggcucc gccuacuaca uguaccucga ccgcuccgac      120

gccggcaagg ccaucuccuu cgccaccacc cucggcguca acaagugcca cguccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucaucaaggu cgagaacugg aucuuccgca accccggcuu cgcccucguc      480

gccgucgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacgucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucacc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucggcu acgagaccga cgaggaccgc gccaaggucg aggucacccc caacuccccc     1080

cgcgccgagg ccacccucgg cggcuucggc ucccucggcc ucgacugcga gccccgcacc     1140

ggccucgacu ucuccgaccu cuacuaccuc accaugaaca acaagcacug gcucguccac     1200

aaggaguggu uccacgacau cccccucccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccucgucgag uucaaggacg cccacgccaa gcgccagacc     1320

gucgucgucc ucggcuccca ggagggcgcc guccacaccg cccucgccgg cgcccucgag     1380

gccgagaugg acggcgccaa gggccgccuc uucuccggcc accucaagug ccgccucaag     1440

auggacaagc uccgccucaa gggcgucucc uacucccucu gcaccgccgc cuucaccuuc     1500

accaaggucc ccgccgagac ccuccacggc accgucaccg ucgaggucca guacgccggc     1560

accgacggcc ccugcaagau ccccguccag auggccgucg acaugcagac ccucaccccc     1620

gucggccgcc ucaucaccgc caaccccguc aucaccgagu ccaccgagaa cuccaagaug     1680

augcucgagc ucgacccccc cuucggcgac uccuacaucg ucaucggcgu cggcgacaag     1740

aagaucaccc accacuggca ccgcuccggc uccacccucg gcaaggccuu cuccaccacc     1800

cucaagggcg cccagcgccu cgccgcccuc ggcgacaccg ccugggacuu cggcuccauc     1860

ggcggcgucu ucaacuccau cggccgcgcc guccaccagg ucuucggcgg cgccuuccgc     1920

acccucuucg gcggcauguc cuggaucacc cagggccuca ugggcgcccu ccuccucugg     1980

augggcguca acgcccgcga ccgcuccauc gcccucgccu uccucgccac cggcggcguc     2040

cucgucuucc ucgccaccaa cguccacgcc                                      2070


<210>  9784
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9784
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgucacccg ccgcggcucc gccuacuaca uguaccucga ccgcaacgac      120

gccggcgagg ccaucuccuu ccccaccacc cucggcauga acaagugcua cauccagauc      180

auggaccucg gccacaugug cgacgccacc auguccuacg agugccccau gcucgacgag      240

ggcgucgagc ccgacgacgu cgacugcugg ugcaacacca ccuccaccug ggucgucuac      300

ggcaccugcc accacaagaa gggcgaggcc cgccgcuccc gccgcgccgu cacccucccc      360

ucccacucca cccgcaagcu ccagacccgc ucccagaccu ggcucgaguc ccgcgaguac      420

accaagcacc ucauccgcgu cgagaacugg aucuuccgca accccggcuu cgcccucgcc      480

gccgccgcca ucgccuggcu ccucggcucc uccaccuccc agaaggucau cuaccucguc      540

augauccucc ucaucgcccc cgccuacucc auccgcugca ucggcgucuc caaccgcgac      600

uucgucgagg gcauguccgg cggcaccugg gucgacgucg uccucgagca cggcggcugc      660

gucaccguca uggcccagga caagcccacc gucgacaucg agcucgucac caccaccguc      720

uccaacaugg ccgagguccg cuccuacugc uacgaggccu ccaucuccga cauggccucc      780

gacucccgcu gccccaccca gggcgaggcc uaccucgaca agcaguccga cacccaguac      840

gucugcaagc gcacccucgu cgaccgcggc uggggcaacg gcugcggccu cuucggcaag      900

ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga agaugaccgg caaguccauc      960

cagcccgaga accucgagua ccgcaucaug cucuccgucc acggcuccca gcacuccggc     1020

augaucguca acgacaccgg ccacgagacc gacgagaacc gcgccaaggu cgagaucacc     1080

cccaacuccc cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc     1140

gagccccgca ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac     1200

uggcucgucc acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc     1320

aagcgccaga ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc     1380

ggcgcccucg aggccgagau ggacggcgcc aagggccgcc ucuccuccgg ccaccucaag     1440

ugccgccuca agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccuccacg gcaccgucac cgucgagguc     1560

caguacgccg gcaccgacgg ccccugcaag guccccgccc agauggccgu cgacaugcag     1620

acccucaccc ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag     1680

aacuccaaga ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc     1740

gucggcgaga agaagaucac ccaccacugg caccgcuccg gcuccacccu cggcaaggcc     1800

uucuccacca cccucaaggg cgcccagcgc cucgccgccc ucggcgacac cgccugggac     1860

uucggcucca ucggcggcgu cuucaacucc aucggccgcg ccguccacca ggucuucggc     1920

ggcgccuucc gcacccucuu cggcggcaug uccuggauca cccagggccu caugggcgcc     1980

cuccuccucu ggaugggcgu caacgcccgc gaccgcucca ucgcccucgc cuuccucgcc     2040

accggcggcg uccucgucuu ccucgccacc aacguccacg cc                        2082


<210>  9785
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9785
augaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgucacccuc       60

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      120

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      180

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      240

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      300

gacuucgucg agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc      360

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      420

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      480

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      540

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      600

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      660

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc      720

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc      780

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac      840

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag      900

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc      960

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1020

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1080

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1140

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1200

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1260

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1320

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1380

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1440

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1500

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1560

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1620

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1680

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     1740

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgcc                     1785


<210>  9786
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9786
augaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgucacccuc       60

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      120

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      180

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      240

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      300

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      360

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      420

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      480

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      540

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      600

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      660

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc      720

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc      780

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac      840

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag      900

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc      960

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1020

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1080

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1140

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1200

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1260

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1320

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1380

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1440

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1500

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1560

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1620

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1680

acccuccuca uguggcucgg ccucaacgcc aagaacggcu ccaucucccu caugugccuc     1740

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgcc                     1785


<210>  9787
<211>  1773
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9787
augaccucca ucggcaucau cggccuccuc cucaccaccg ccauggccgc cgucacccuc       60

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      120

uacaccaagc accucaucaa ggucgagaac uggaucuucc gcaaccccgg cuucgcccuc      180

gucgccgucg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      240

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      300

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      360

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      420

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      480

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      540

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      600

aagggcuccc ucgucaccug cgccaaguuc accugcucca agaagaugac cggcaagucc      660

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc      720

ggcaugaucg gcuacgagac cgacgaggac cgcgccaagg ucgaggucac ccccaacucc      780

ccccgcgccg aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc      840

accggccucg acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc      900

cacaaggagu gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc      960

ccccacugga acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag     1020

accgucgucg uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc     1080

gaggccgaga uggacggcgc caagggccgc cucuucuccg gccaccucaa gugccgccuc     1140

aagauggaca agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc     1200

uucaccaagg uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc     1260

ggcaccgacg gccccugcaa gauccccguc cagauggccg ucgacaugca gacccucacc     1320

cccgucggcc gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag     1380

augaugcucg agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgac     1440

aagaagauca cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc     1500

accguccgcg gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc     1560

gucggcggcg ucuucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc     1620

aagucccucu ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucguc     1680

uggcucggcc ucaacaccaa gaacggcucc aucucccuca ccugccucgc ccucggcggc     1740

gucaugaucu uccucuccac cgccgucucc gcc                                  1773


<210>  9788
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9788
augaccuccg ucggcaucgu cggccuccuc cucaccaccg ccauggccgc cgucacccuc       60

cccucccacu ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag      120

uacaccaagc accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc      180

gccgccgccg ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc      240

gucaugaucc uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc      300

gacuucgucg agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc      360

ugcgucaccg ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc      420

gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc      480

uccgacuccc gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag      540

uacgucugca agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc      600

aagggcuccc ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc      660

auccagcccg agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc      720

ggcaugaucg ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc      780

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac      840

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag      900

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc      960

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1020

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1080

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc     1140

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1200

gccgccuuca ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag     1260

guccaguacg ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug     1320

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1380

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1440

ggcgucggcg agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1500

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1560

gacuucggcu ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc     1620

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1680

acccuccuca uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc     1740

gcccucggcg gcguccucau cuuccucucc accgccgucu ccgcc                     1785


<210>  9789
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9789
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      120

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      180

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      240

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      300

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      360

gucgacaucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      420

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      480

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      540

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      600

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      660

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug      720

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc      780

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc      840

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      900

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      960

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1020

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1080

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1140

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1200

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1260

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1320

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1380

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1440

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1500

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1560

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1620

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1680

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     1740

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     1800

gccgucuccg cc                                                         1812


<210>  9790
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9790
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      120

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      180

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      240

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      300

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      360

gucgacgucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      420

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      480

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      540

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      600

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      660

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug      720

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc      780

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc      840

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      900

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      960

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1020

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1080

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1140

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1200

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1260

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1320

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1380

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1440

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1500

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1560

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1620

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1680

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacgccaag     1740

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     1800

gccgucuccg cc                                                         1812


<210>  9791
<211>  1800
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9791
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      120

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg      180

aucuuccgca accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc      240

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      300

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      360

gucgacgucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      420

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      480

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      540

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      600

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc      660

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug      720

cucuccgucc acggcuccca gcacuccggc augaucggcu acgagaccga cgaggaccgc      780

gccaaggucg aggucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc      840

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc      900

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc      960

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1020

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1080

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1140

uucuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1200

uacucccucu gcaccgccgc cuucaccuuc accaaggucc ccgccgagac ccuccacggc     1260

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaagau ccccguccag     1320

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1380

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1440

uccuacaucg ucaucggcgu cggcgacaag aagaucaccc accacuggca ccgcuccggc     1500

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1560

ggcgacaccg ccugggacuu cggcuccguc ggcggcgucu ucaacucccu cggcaagggc     1620

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1680

cagauccuca ucggcacccu ccucgucugg cucggccuca acaccaagaa cggcuccauc     1740

ucccucaccu gccucgcccu cggcggcguc augaucuucc ucuccaccgc cgucuccgcc     1800


<210>  9792
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9792
auggccaucu ccggcguccc cguccucggc uucuucauca ucgccguccu cauguccgcc       60

caggaguccu gggccgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc      120

ucccagaccu ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg      180

aucuuccgca accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc      240

uccaccuccc agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc      300

auccgcugca ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg      360

gucgacgucg uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc      420

gucgacaucg agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc      480

uacgaggccu ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc      540

uaccucgaca agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc      600

uggggcaacg gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc      660

ugcuccaaga agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug      720

cucuccgucc acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc      780

gacgagaacc gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc      840

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      900

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      960

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1020

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1080

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1140

aagggccgcc ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1200

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1260

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1320

guccccgccc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1380

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1440

cccuucggcg acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg     1500

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1560

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc     1620

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1680

uccugguucu cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag     1740

aacggcucca ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc     1800

gccgucuccg cc                                                         1812


<210>  9793
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9793
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgccguc       60

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      120

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      180

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      240

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      300

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacaucgu ccucgagcac      360

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      420

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      480

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      540

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      600

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      660

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag      720

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc      780

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc      840

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac      900

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc      960

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1020

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1080

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1140

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1200

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1260

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1320

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1380

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1440

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1500

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1560

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1620

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1680

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     1740

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc c              1791


<210>  9794
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9794
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgccguc       60

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      120

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      180

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      240

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      300

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacgucgu ccucgagcac      360

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      420

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      480

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      540

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      600

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      660

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag      720

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc      780

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc      840

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac      900

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc      960

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1020

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1080

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1140

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1200

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1260

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1320

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1380

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1440

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1500

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1560

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1620

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1680

aucggcaccc uccucaugug gcucggccuc aacgccaaga acggcuccau cucccucaug     1740

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc c              1791


<210>  9795
<211>  1779
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9795
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgccguc       60

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      120

cgcgaguaca ccaagcaccu caucaagguc gagaacugga ucuuccgcaa ccccggcuuc      180

gcccucgucg ccgucgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      240

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      300

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacgucgu ccucgagcac      360

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      420

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      480

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      540

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      600

uucggcaagg gcucccucgu caccugcgcc aaguucaccu gcuccaagaa gaugaccggc      660

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag      720

cacuccggca ugaucggcua cgagaccgac gaggaccgcg ccaaggucga ggucaccccc      780

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag      840

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg      900

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc      960

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1020

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1080

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ucuccggcca ccucaagugc     1140

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1200

uucaccuuca ccaagguccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1260

uacgccggca ccgacggccc cugcaagauc cccguccaga uggccgucga caugcagacc     1320

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1380

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1440

ggcgacaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1500

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1560

ggcuccgucg gcggcgucuu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1620

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1680

cucgucuggc ucggccucaa caccaagaac ggcuccaucu cccucaccug ccucgcccuc     1740

ggcggcguca ugaucuuccu cuccaccgcc gucuccgcc                            1779


<210>  9796
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9796
auggacugga ccuggauccu cuuccucguc gccgccgcca cccgcgucca cuccgccguc       60

acccuccccu cccacuccac ccgcaagcuc cagacccgcu cccagaccug gcucgagucc      120

cgcgaguaca ccaagcaccu cauccgcguc gagaacugga ucuuccgcaa ccccggcuuc      180

gcccucgccg ccgccgccau cgccuggcuc cucggcuccu ccaccuccca gaaggucauc      240

uaccucguca ugauccuccu caucgccccc gccuacucca uccgcugcau cggcgucucc      300

aaccgcgacu ucgucgaggg cauguccggc ggcaccuggg ucgacgucgu ccucgagcac      360

ggcggcugcg ucaccgucau ggcccaggac aagcccaccg ucgacaucga gcucgucacc      420

accaccgucu ccaacauggc cgagguccgc uccuacugcu acgaggccuc caucuccgac      480

auggccuccg acucccgcug ccccacccag ggcgaggccu accucgacaa gcaguccgac      540

acccaguacg ucugcaagcg cacccucguc gaccgcggcu ggggcaacgg cugcggccuc      600

uucggcaagg gcucccucgu caccugcgcc aaguucgccu gcuccaagaa gaugaccggc      660

aaguccaucc agcccgagaa ccucgaguac cgcaucaugc ucuccgucca cggcucccag      720

cacuccggca ugaucgucaa cgacaccggc cacgagaccg acgagaaccg cgccaagguc      780

gagaucaccc ccaacucccc ccgcgccgag gccacccucg gcggcuucgg cucccucggc      840

cucgacugcg agccccgcac cggccucgac uucuccgacc ucuacuaccu caccaugaac      900

aacaagcacu ggcucgucca caaggagugg uuccacgaca ucccccuccc cuggcacgcc      960

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccucgucga guucaaggac     1020

gcccacgcca agcgccagac cgucgucguc cucggcuccc aggagggcgc cguccacacc     1080

gcccucgccg gcgcccucga ggccgagaug gacggcgcca agggccgccu cuccuccggc     1140

caccucaagu gccgccucaa gauggacaag cuccgccuca agggcgucuc cuacucccuc     1200

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccuccacgg caccgucacc     1260

gucgaggucc aguacgccgg caccgacggc cccugcaagg uccccgccca gauggccguc     1320

gacaugcaga cccucacccc cgucggccgc cucaucaccg ccaaccccgu caucaccgag     1380

uccaccgaga acuccaagau gaugcucgag cucgaccccc ccuucggcga cuccuacauc     1440

gucaucggcg ucggcgagaa gaagaucacc caccacuggc accgcuccgg cuccaccauc     1500

ggcaaggccu ucgaggccac cguccgcggc gccaagcgca uggccguccu cggcgacacc     1560

gccugggacu ucggcuccgu cggcggcgcc cucaacuccc ucggcaaggg cauccaccag     1620

aucuucggcg ccgccuucaa gucccucuuc ggcggcaugu ccugguucuc ccagauccuc     1680

aucggcaccc uccucaugug gcucggccuc aacaccaaga acggcuccau cucccucaug     1740

ugccucgccc ucggcggcgu ccucaucuuc cucuccaccg ccgucuccgc c              1791


<210>  9797
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9797
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      120

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      180

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      240

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      300

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      360

gacaucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      420

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      480

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      540

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      600

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      660

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc      720

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac      780

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc      840

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc      900

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc      960

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1020

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1080

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1140

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1200

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1260

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1320

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1380

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1440

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1500

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1560

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1620

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1680

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     1740

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     1800

gucuccgcc                                                             1809


<210>  9798
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9798
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      120

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      180

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      240

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      300

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      360

gacgucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      420

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      480

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      540

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      600

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      660

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc      720

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac      780

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc      840

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc      900

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc      960

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1020

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1080

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1140

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1200

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1260

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1320

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1380

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1440

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1500

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1560

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1620

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1680

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa cgccaagaac     1740

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     1800

gucuccgcc                                                             1809


<210>  9799
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9799
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      120

cagaccuggc ucgagucccg cgaguacacc aagcaccuca ucaaggucga gaacuggauc      180

uuccgcaacc ccggcuucgc ccucgucgcc gucgccaucg ccuggcuccu cggcuccucc      240

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      300

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      360

gacgucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      420

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      480

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      540

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      600

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucaccugc      660

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc      720

uccguccacg gcucccagca cuccggcaug aucggcuacg agaccgacga ggaccgcgcc      780

aaggucgagg ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc      840

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc      900

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg      960

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1020

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1080

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucuuc     1140

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1200

ucccucugca ccgccgccuu caccuucacc aagguccccg ccgagacccu ccacggcacc     1260

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagauccc cguccagaug     1320

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1380

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1440

uacaucguca ucggcgucgg cgacaagaag aucacccacc acuggcaccg cuccggcucc     1500

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1560

gacaccgccu gggacuucgg cuccgucggc ggcgucuuca acucccucgg caagggcauc     1620

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1680

auccucaucg gcacccuccu cgucuggcuc ggccucaaca ccaagaacgg cuccaucucc     1740

cucaccugcc ucgcccucgg cggcgucaug aucuuccucu ccaccgccgu cuccgcc        1797


<210>  9800
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9800
augggcaagc gcuccgccgg cuccaucaug uggcucgccu cccucgccgu cgucaucgcc       60

ugcgccggcg ccgccgucac ccuccccucc cacuccaccc gcaagcucca gacccgcucc      120

cagaccuggc ucgagucccg cgaguacacc aagcaccuca uccgcgucga gaacuggauc      180

uuccgcaacc ccggcuucgc ccucgccgcc gccgccaucg ccuggcuccu cggcuccucc      240

accucccaga aggucaucua ccucgucaug auccuccuca ucgcccccgc cuacuccauc      300

cgcugcaucg gcgucuccaa ccgcgacuuc gucgagggca uguccggcgg caccuggguc      360

gacgucgucc ucgagcacgg cggcugcguc accgucaugg cccaggacaa gcccaccguc      420

gacaucgagc ucgucaccac caccgucucc aacauggccg agguccgcuc cuacugcuac      480

gaggccucca ucuccgacau ggccuccgac ucccgcugcc ccacccaggg cgaggccuac      540

cucgacaagc aguccgacac ccaguacguc ugcaagcgca cccucgucga ccgcggcugg      600

ggcaacggcu gcggccucuu cggcaagggc ucccucguca ccugcgccaa guucgccugc      660

uccaagaaga ugaccggcaa guccauccag cccgagaacc ucgaguaccg caucaugcuc      720

uccguccacg gcucccagca cuccggcaug aucgucaacg acaccggcca cgagaccgac      780

gagaaccgcg ccaaggucga gaucaccccc aacucccccc gcgccgaggc cacccucggc      840

ggcuucggcu cccucggccu cgacugcgag ccccgcaccg gccucgacuu cuccgaccuc      900

uacuaccuca ccaugaacaa caagcacugg cucguccaca aggagugguu ccacgacauc      960

ccccuccccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1020

cucgucgagu ucaaggacgc ccacgccaag cgccagaccg ucgucguccu cggcucccag     1080

gagggcgccg uccacaccgc ccucgccggc gcccucgagg ccgagaugga cggcgccaag     1140

ggccgccucu ccuccggcca ccucaagugc cgccucaaga uggacaagcu ccgccucaag     1200

ggcgucuccu acucccucug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1260

cuccacggca ccgucaccgu cgagguccag uacgccggca ccgacggccc cugcaagguc     1320

cccgcccaga uggccgucga caugcagacc cucacccccg ucggccgccu caucaccgcc     1380

aaccccguca ucaccgaguc caccgagaac uccaagauga ugcucgagcu cgaccccccc     1440

uucggcgacu ccuacaucgu caucggcguc ggcgagaaga agaucaccca ccacuggcac     1500

cgcuccggcu ccaccaucgg caaggccuuc gaggccaccg uccgcggcgc caagcgcaug     1560

gccguccucg gcgacaccgc cugggacuuc ggcuccgucg gcggcgcccu caacucccuc     1620

ggcaagggca uccaccagau cuucggcgcc gccuucaagu cccucuucgg cggcaugucc     1680

ugguucuccc agauccucau cggcacccuc cucauguggc ucggccucaa caccaagaac     1740

ggcuccaucu cccucaugug ccucgcccuc ggcggcgucc ucaucuuccu cuccaccgcc     1800

gucuccgcc                                                             1809


<210>  9801
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9801
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgugac cagaagggga ucagcguacu auauguaccu ugaccggaac      120

gaugcuggug aagccaucag uuucccuaca acucugggca ugaauaagug cuauauucaa      180

aucauggacu uggggcacau gugugaugca acgaugagcu acgagugccc aauguuagac      240

gaaggaguag agccggauga cguggauugu uggugcaaca ccacauccac uugggucgug      300

uauggcaccu gucaucacaa aaagggugaa gcccgccgau cucguagagc uguuacacug      360

cccucgcauu caaccaggaa acuacagacu cggagccaga cauggcucga gagucgcgag      420

uacaccaagc accugauuag aguggaaaau uggaucuuua ggaacccugg guucgcacuu      480

gccgcugccg cgauagcaug gcuguuggga uccucuacga gccagaaagu caucuaucuc      540

gugaugauuc ugcugaucgc cccagcuuac uccauucggu gcaucggcgu aucaaaucgc      600

gacuuuguug agggcaugag ugggggaacu uggguggaua uaguccucga acaugguggc      660

ugugugaccg ugauggccca agacaagccc acagucgaua uugaguuagu uacuaccaca      720

gugagcaaca uggcagaggu acgaucuuac ugcuaugagg cuuccaucuc agacauggcc      780

agcgauagua gauguccuac ccagggggaa gcguaccugg acaagcaguc ugacacgcag      840

uaugugugca aaaggacucu ugucgaucgg ggauggggca augguugugg guuguucgga      900

aagggcuccc uggugacaug cgccaaauuu gcaugcagca agaaaaugac cggcaagucg      960

auucaaccag aaaaccuaga guaccguauc augcucucug uucacggguc ccagcacuca     1020

ggaaugaucg ugaaugacac uggucaugaa acagaugaga accgcgcuaa ggucgagauu     1080

acccccaaua guccuagagc cgaagcuacg cugggcgggu ucggaagccu uggccuggac     1140

ugugagccaa ggaccgguuu ggauuuuucc gaccucuauu accugacaau gaacaauaaa     1200

cacugguuag ugcauaagga augguuccac gauauaccgc ugcccuggca cgccggggca     1260

gacacuggca ccccucauug gaacaauaaa gaggcccucg uagaauuuaa ggaugcucac     1320

gcgaaacggc agacaguugu gguccuugga ucucaggagg gcgcagugca uacugcccug     1380

gcuggggccu uggaggcaga aauggacgga gccaaggguc gacuaagcuc aggccaccug     1440

aaaugccgcc ucaagaugga uaagcugaga cuuaaagggg ucaguuauuc ccuguguacc     1500

gcugccuuca cauuuaccaa gaucccagca gagacuuugc auggaacggu gacaguugaa     1560

gugcaauacg cgggcaccga cggucccugc aaaguaccug cucagauggc cgucgacaug     1620

cagacucuca ccccaguggg gaggcugauu acagcaaacc ccguuaucac cgagagcacu     1680

gaaaacucua agaugauguu agagcuggau ccgccuuucg gcgacucaua cauagugauu     1740

ggagucggcg agaaaaagau cacacaccac uggcaucgga gugggagcac gaucggaaag     1800

gccuucgaag cuaccgugcg cggugccaaa agaauggcag uacuaggcga uacugcuugg     1860

gacuuugggu ccgugggagg cgcccucaau ucucugggua aggggauuca ccaaaucuuc     1920

ggcgcggccu uuaaaucgcu uuucggaggc augagcuggu uuucccagau uuugauaggg     1980

acacugcuca uguggcuggg acuuaacacc aagaauggcu caaucagucu gauguguuug     2040

gcucucggug ggguucugau uuucuuaucu acagcaguca gcgcc                     2085


<210>  9802
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9802
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgugac cagaagggga ucagcguacu auauguaccu ugaccggaac      120

gaugcuggug aagccaucag uuucccuaca acucugggca ugaauaagug cuauauucaa      180

aucauggacu uggggcacac gugugaugca accaugagcu acgagugccc aauguuagac      240

gaaggaguag agccggauga cguggauugu uggugcaaca caacuuccac cugggucgug      300

uauggcacau gucaucacaa aaagggugaa gcccgccgau cucguagagc uguuacccug      360

cccucgcauu caacuaggaa acuacagaca cggagccaga ccuggcucga gagucgcgag      420

uacacgaagc accugauuag aguggaaaau uggaucuuua ggaacccugg guucgcacuu      480

gccgcugccg cgauagcaug gcuguuggga uccucuacua gccagaaagu caucuaucuc      540

gugaugauuc ugcugaucgc cccagcuuac uccauucggu gcaucggcgu aucaaaucgc      600

gacuuuguug agggcaugag ugggggaacc uggguggaug ucgugcucga acaugguggc      660

ugugugacag ucauggccca agacaagccc acuguugaua uagaguuagu gaccacaacc      720

guaagcaaca uggcagaggu gcgaucuuac ugcuaugagg cuuccauuuc agacauggcc      780

agcgauagua gauguccuac gcagggggaa gcguaccugg acaagcaguc ugacacucag      840

uaugucugca aaaggacacu uguggaucgg ggauggggca augguugugg guuguucgga      900

aagggcuccc ugguuaccug cgccaaauuu gcaugcagca agaaaaugac uggcaagucg      960

auccaaccag aaaaccuaga guaccguauu augcucucug ugcacggguc ccagcacuca     1020

ggaaugaucg ucaaugacac aggucaugaa accgaugaga accgcgcuaa gguggagauc     1080

acgcccaaua guccuagagc cgaagcuacc cugggcgggu ucggaagccu uggccuggac     1140

ugugagccaa ggacagguuu ggauuuuucc gaccucuauu accugacuau gaacaauaaa     1200

cacugguuag uacauaagga augguuccac gauauuccgc ugcccuggca cgccggggca     1260

gacaccggca caccucauug gaacaauaaa gaggcccucg uugaauuuaa ggaugcucac     1320

gcgaaacggc agacuguggu cgugcuugga ucucaggagg gcgcagucca uaccgcccug     1380

gcuggggccu uggaggcaga aauggacgga gccaaggguc gacuaagcuc aggccaccug     1440

aaaugccgcc ucaagaugga uaagcugaga cuuaaagggg ugaguuauuc ccuguguaca     1500

gcugccuuca ccuuuacuaa gauaccagca gagacguugc auggaacagu uaccguggaa     1560

guacaauacg cgggcacuga cggucccugc aaagucccug cucagauggc cguggacaug     1620

cagacccuca caccaguugg gaggcugauc accgcaaacc ccgugauuac ugagagcaca     1680

gaaaacucua agaugauguu agagcuggau ccgccuuucg gcgacucaua caucgucaua     1740

ggagugggcg agaaaaagau uacgcaccac uggcaucgga gugggagcac caucggaaag     1800

gccuucgaag cuacuguacg cggugccaaa agaauggcag ugcuaggcga uacagcuugg     1860

gacuuugggu ccguuggagg cgcccucaau ucucugggua aggggaucca ccaaauuuuc     1920

ggcgcggccu uuaaaucgcu uuucggaggc augagcuggu uuucccagau cuugauuggg     1980

acccugcuca uguggcuggg acuuaacgcu aagaauggcu caauaagucu gauguguuug     2040

gcacucggug ggguccugau cuucuuaucu acagccguga gcgcu                     2085


<210>  9803
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9803
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccaucac cagaagggga ucagcguacu auauguaccu ugaccggagu      120

gaugcuggua aggccauuag cuucgcaaca acucugggcg ugaacaaaug ccacguacaa      180

aucauggacu uggggcauau gugugaugcc acgauguccu augaaugccc uauguuagac      240

gagggagugg aaccagauga cgucgauugu uggugcaaua ccacaucuac uugggugguu      300

uacggcaccu gucaccauaa gaaaggugag gcucgccgau cgcguagagc agugacacug      360

ccgucacaca gcaccaggaa gcuacagacu cggagucaga cauggcucga aucccgcgag      420

uauaccaaac aucugauuaa ggucgagaac uggaucuuua gaaaucccgg guucgcccuu      480

guggcuguag ccauagcgug gcuguuggga ucuagcacgu cccagaaggu uaucuaccuc      540

gugaugauuc ugcugaucgc acccgccuau ucaauuaggu gcaucggcgu caguaaccgg      600

gacuuugugg aaggcaugag cgggggaacu uggguggaug ucguucucga gcacgguggc      660

ugugugaccg uaauggcuca agacaaacca acaguggaua uagaauuagu cacuaccaca      720

gugucuaaua uggccgaggu ucgcuccuac ugcuacgagg caucaauuag cgacauggcu      780

agugauucuc gaugucccac ccagggggaa gccuaucugg acaagcaguc cgacacgcag      840

uacgugugca aaagaacucu ugucgauagg ggauggggca acgguugugg guuguucgga      900

aagggcagcc uggugacaug cgcgaaauuu accugcucga agaagaugac uggcaaaucu      960

auccaaccug agaaucuaga auaucggauu augcucuccg uacacggguc acagcauagu     1020

ggaaugaucg guuacgagac agacgaagau cgugccaagg uugaggugac cccaaacagc     1080

ccccgcgcag aggcuacgcu gggcggguuc ggaucccuug gccuggacug ugaaccuaga     1140

accgguuugg auuuuucuga ccucuauuac cugacaauga auaacaaaca cugguuaguc     1200

cauaaggagu gguuccacga uaucccacug ccguggcacg ccggggcuga cacuggcacc     1260

ccucauugga auaacaaaga agcccucgug gaguuuaagg augcacacgc caaaaggcag     1320

acagucgugg uucuuggaag ccaggaaggc gcugugcaua cugcgcuggc aggggccuug     1380

gaggcugaga uggacggagc caagggucgg cuauucucag gccaccugaa gugccgacuc     1440

aaaauggaua agcugcgccu uaaaggggua aguuauuccc uguguaccgc agccuuuaca     1500

uucaccaagg ucccugcuga aacuuugcau ggaacgguga caguugaggu gcaauacgcc     1560

ggcaccgacg guccaugcaa aauucccguc cagauggcag uggacaugca gacucucacc     1620

ccuguaggga gacugauaac agcgaaucca gugaucaccg aaagcacuga gaacucuaag     1680

augauguuag aacuggaucc cccguucggc gacucauaca uuguuaucgg agucggcgau     1740

aagaaaauaa cacaccacug gcauaggagu gggagcacga uuggaaaggc uuuugaggcc     1800

accgugcggg gugcaaaacg cauggccgug cuaggcgaca cugcuuggga uuucgggucc     1860

gucggaggcg uuuuuaauuc ucucgguaag gggauccacc aaaucuucgg cgccgcauuu     1920

aaaucgcugu ucggaggcau gagcugguuu ucccagauuc uuaucgggac auugcuggug     1980

uggcucggac ugaacaccaa gaacggcuca auuagucuua caugucuggc uuuggguggg     2040

gugaugauau uccucucuac ugccguaagc gcg                                  2073


<210>  9804
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9804
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgugac cagaagggga ucagcguacu auauguaccu ugaccggaac      120

gaugcuggug aagccaucag uuucccuaca acucugggca ugaauaagug cuauauucaa      180

aucauggacu uggggcacau gugugaugca acgaugagcu acgagugccc aauguuagac      240

gaaggaguag agccggauga cguggauugu uggugcaaca ccacauccac uugggucgug      300

uauggcaccu gucaucacaa aaagggugaa gcccgccgau cucguagagc uguuacacug      360

cccucgcauu caaccaggaa acuacagacu cggagccaga cauggcucga gagucgcgag      420

uacaccaagc accugauuag aguggaaaau uggaucuuua ggaacccugg guucgcacuu      480

gccgcugccg cgauagcaug gcuguuggga uccucuacga gccagaaagu caucuaucuc      540

gugaugauuc ugcugaucgc cccagcuuac uccauucggu gcaucggcgu aucaaaucgc      600

gacuuuguug agggcaugag ugggggaacu uggguggaug ucgugcucga acaugguggc      660

ugugugaccg ucauggccca agacaagccc acaguugaua uagaguuagu gacuaccaca      720

guaagcaaca uggcagaggu gcgaucuuac ugcuaugagg cuuccauuuc agacauggcc      780

agcgauagua gauguccuac ccagggggaa gcguaccugg acaagcaguc ugacacgcag      840

uaugucugca aaaggacucu uguggaucgg ggauggggca augguugugg guuguucgga      900

aagggcuccc ugguuacaug cgccaaauuu gcaugcagca agaaaaugac cggcaagucg      960

auccaaccag aaaaccuaga guaccguauu augcucucug ugcacggguc ccagcacuca     1020

ggaaugaucg ucaaugacac uggucaugaa acagaugaga accgcgcuaa gguggagauc     1080

acccccaaua guccuagagc cgaagcuacg cugggcgggu ucggaagccu uggccuggac     1140

ugugagccaa ggaccgguuu ggauuuuucc gaccucuauu accugacaau gaacaauaaa     1200

cacugguuag uacauaagga augguuccac gauauuccgc ugcccuggca cgccggggca     1260

gacacuggca ccccucauug gaacaauaaa gaggcccucg uugaauuuaa ggaugcucac     1320

gcgaaacggc agacaguggu cgugcuugga ucucaggagg gcgcagucca uacugcccug     1380

gcuggggccu uggaggcaga aauggacgga gccaaggguc gacuaagcuc aggccaccug     1440

aaaugccgcc ucaagaugga uaagcugaga cuuaaagggg ugaguuauuc ccuguguacc     1500

gcugccuuca cauuuaccaa gauaccagca gagacuuugc auggaacggu uacaguggaa     1560

guacaauacg cgggcaccga cggucccugc aaagucccug cucagauggc cguggacaug     1620

cagacucuca ccccaguugg gaggcugauc acagcaaacc ccgugauuac cgagagcacu     1680

gaaaacucua agaugauguu agagcuggau ccgccuuucg gcgacucaua caucgucaua     1740

ggagugggcg agaaaaagau uacacaccac uggcaucgga gugggagcac gaucggaaag     1800

gccuucgaag cuaccguacg cggugccaaa agaauggcag ugcuaggcga uacugcuugg     1860

gacuuugggu ccguuggagg cgcccucaau ucucugggua aggggaucca ccaaauuuuc     1920

ggcgcggccu uuaaaucgcu uuucggaggc augagcuggu uuucccagau cuugauuggg     1980

acacugcuca uguggcuggg acuuaacacc aagaauggcu caauaagucu gauguguuug     2040

gcucucggug ggguccugau cuucuuaucu acagcaguga gcgcc                     2085


<210>  9805
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9805
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcguuacu       60

aggcggggcu ccgccuacua uauguaccuu gaucgcaacg acgcggggga ggcuauuucu      120

uuucccacga cccugggaau gaauaagugc uauauacaga ucauggauuu gggucauaug      180

ugugacgcca caaugucaua cgaaugcccu auguuagaug agggcgugga accagacgau      240

guagacuguu ggugcaacac uaccaguaca uggguggucu augggaccug ucaccauaaa      300

aagggagagg cacgacguag cagaagggcc gugacucugc cgucccacuc uacacggaaa      360

cuacaaaccc gcucgcagac guggcucgaa ucaagagagu acacuaagca ucugauuagg      420

guugagaauu ggaucuuccg gaaccccggc uuugcucuug cagccgcugc cauugcgugg      480

cuguugggua gcaguaccuc ccagaaagug aucuaucucg ucaugauacu gcugaucgca      540

cccgccuacu cuauucgcug caucggggug agcaaucgag auuucguaga aggaaugucc      600

ggcggcacau ggguugacau ugugcucgag cacgggggau gugucacugu gauggcucag      660

gauaagccaa ccguggacau cgaauuaguc acaaccacgg uuucaaacau ggccgaggug      720

agaaguuacu gcuaugaggc aagcauaucu gauauggcuu ccgacucaag gugucccacu      780

caaggugaag ccuaccugga caagcagagc gauacacagu auguaugcaa acggacccuu      840

guggaccgug gcugggggaa uggauguggc uuguuuggua aggggagucu ggucacuugc      900

gcgaaauucg ccugcucuaa gaaaaugaca ggaaagucca uucagccuga gaaccuagaa      960

uaccgcauca ugcucagcgu gcacggcucg caacauucug gcaugauugu uaaugauacc     1020

gggcacgaga cggacgaaaa cagagcaaag guggagauca ccccaaauuc ccccagggcu     1080

gaggccacac ugggagguuu uggcucacuu gggcuggauu gugaaccucg gacuggauug     1140

gacuucagug aucucuauua ccugaccaug aacaauaaac auugguuagu ccacaaggag     1200

ugguuucacg acaucccacu gccguggcau gcuggcgccg auacagguac uccccacugg     1260

aacaauaaag aagcacucgu ggaguucaag gacgcccaug cuaaacgaca gaccguaguu     1320

gugcuuggga gccaggaagg cgcgguccac acagcacugg ccggagcuuu ggaggccgag     1380

auggauggcg caaaggggcg ccuauccucu ggacaucuga aaugcagacu caagauggac     1440

aagcugaggc uuaaaggugu gagcuauuca cuguguaccg ccgcuuuuac uuucacgaag     1500

auuccugccg aaacauugca cggcaccguc acuguggagg uucaguacgc agggaccgac     1560

ggaccaugca aagugcccgc gcaaauggcu guagauaugc agacacucac cccugucggc     1620

cggcugauaa cugccaaccc agugaucaca gaaaguacgg agaacuccaa gaugauguua     1680

gaacuggacc cuccguuugg ugauagcuac auuguuaucg gggugggcga gaaaaagaua     1740

acccaccauu ggcaccgcuc uggaucaacu auuggcaagg cauucgaggc cacagucaga     1800

ggggcuaaaa ggauggccgu gcuaggagac accgcauggg auuucgguag uguaggcggg     1860

gcucucaaua gccugggaaa gggcauccau cagaucuuug gugccgcguu caaaucccuu     1920

uuugggggca ugucuugguu cucgcaaauu uugaucggaa cacugcucau guggcugggc     1980

cuuaacacua agaaugggag cauuucccug auguguuugg cccucggagg cgugcugaua     2040

uuuuuaucaa ccgcuguuag ugca                                            2064


<210>  9806
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9806
auggacugga ccuggauucu guuccucgug gccgcugcaa caagagucca cagcguuacu       60

aggcggggcu ccgccuacua uauguaccuu gaucgcaacg acgcggggga ggcuauuucu      120

uuucccacga cccugggaau gaauaagugc uauauacaga ucauggauuu gggucauaca      180

ugugacgcca cuaugucaua cgaaugcccu auguuagaug agggcgugga accagacgau      240

guagacuguu ggugcaacac cacaaguacc uggguggucu augggacuug ucaccauaaa      300

aagggagagg cacgacguag cagaagggcc gugacacugc cgucccacuc uacccggaaa      360

cuacaaacgc gcucgcagac uuggcucgaa ucaagagagu acaccaagca ucugauuagg      420

guugagaauu ggaucuuccg gaaccccggc uuugcucuug cagccgcugc cauugcgugg      480

cuguugggua gcaguacauc ccagaaagug aucuaucucg ucaugauacu gcugaucgca      540

cccgccuacu cuauucgcug caucggggug agcaaucgag auuucguaga aggaaugucc      600

ggcggcacuu ggguugacgu gguccucgag cacgggggau gugugaccgu gauggcucag      660

gauaagccaa cagucgacau ugaauuaguu accacgacug ugucaaacau ggccgaggua      720

agaaguuacu gcuaugaggc aagcaucucu gauauggcuu ccgacucaag gugucccaca      780

caaggugaag ccuaccugga caagcagagc gauacccagu augugugcaa acggacucuu      840

gucgaccgug gcugggggaa uggauguggc uuguuuggua aggggagucu ggugacaugc      900

gcgaaauucg ccugcucuaa gaaaaugacc ggaaagucca uacagccuga gaaccuagaa      960

uaccgcauua ugcucagcgu ucacggcucg caacauucug gcaugaucgu gaaugauacg     1020

gggcacgaga ccgacgaaaa cagagcaaag gucgagauua caccaaauuc ccccagggcu     1080

gaggccacuc ugggagguuu uggcucacuu gggcuggauu gugaaccucg gaccggauug     1140

gacuucagug aucucuauua ccugacaaug aacaauaaac auugguuagu gcacaaggag     1200

ugguuucacg acaucccacu gccguggcau gcuggcgccg auacugguac cccucacugg     1260

aacaauaaag aagcacucgu agaguucaag gacgcccaug cuaaacgaca gacaguugug     1320

guccuuggga gccaggaagg cgcggugcac accgcacugg ccggagcuuu ggaggccgag     1380

auggauggcg caaaggggcg ccuauccucu ggacaucuga aaugcagacu caagauggac     1440

aagcugaggc uuaaaggugu cagcuauuca cuguguacug ccgcuuuuac guucacaaag     1500

aucccugccg aaaccuugca cggcacugug accguugagg ugcaguacgc agggacagac     1560

ggaccaugca aaguacccgc gcaaauggcu gucgauaugc agacccucac uccugugggc     1620

cggcugauua cagccaaccc aguuauaacg gaaaguaccg agaacuccaa gaugauguua     1680

gaacuggauc ccccguuugg ugacagcuac aucgugauug gggucggcga gaaaaagauc     1740

acucaccauu ggcaccgcuc uggaucaaca auaggcaagg cauucgaggc caccgugaga     1800

ggggcuaaaa ggauggccgu acuaggagac acagcauggg auuucgguag ugugggcggg     1860

gcucucaaua gccugggaaa gggcauucau cagaucuuug gugccgcguu caaaucccuu     1920

uuugggggca ugucuugguu cucgcaaauc uugauuggaa cucugcucau guggcugggc     1980

cuuaacgcca agaaugggag caucucccug auguguuugg cucucggagg cguucugauc     2040

uuuuuaucaa ccgcagucag ugcc                                            2064


<210>  9807
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9807
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcauuacu       60

aggcggggcu ccgccuacua uauguaccuu gaucgcucug acgcggggaa ggcuauauca      120

uuugccacga cccugggagu uaacaaaugc caugugcaga ucauggauuu gggucacaug      180

ugugacgcaa caaugaguua ugagugcccc auguuagaug aaggcguaga gccugacgau      240

guggacuguu ggugcaauac uaccagcaca ugggucgugu acgggaccug ucaucacaag      300

aaaggagaag cccgacguuc cagaagggcu guuacucugc caucucauuc gacacggaag      360

cuacaaaccc gcucacagac guggcucgag agcagagaau auacuaaaca ccugauuaag      420

guggagaacu ggaucuucag gaauccgggc uuugcacuug ucgccguggc uauugccugg      480

cuguugggua guuccaccuc ucagaaggua aucuaccucg uuaugauacu gcugaucgcg      540

cccgcauaua gcauucggug caucggggug uccaaccgcg auuucgucga gggaauguca      600

ggcggcacau ggguggacgu gguccucgaa cacgggggau guguuacugu gauggcccag      660

gauaaaccua ccguagacau ugaguuagug acaaccacgg ucaguaauau ggcugaagug      720

cgaagcuacu gcuacgaggc cucuaucucc gauauggcau cagacagcag auguccaacu      780

caaggugagg cuuaucugga caagcagagu gauacacagu acguuugcaa aaggacccuu      840

guggaccggg gcugggggaa cggauguggc uuguuuggua aggggucucu ggucacuugc      900

gccaaauuca caugcuccaa gaagaugacc ggaaaaagca uacagcccga aaaucuagag      960

uaucguauua ugcucucggu gcauggcucu caacacuccg gcaugaucgg guacgaaacg     1020

gaugaggacc gcgcgaaggu agaaguuacc ccuaacucac caagagccga ggcaacacug     1080

ggagguuuug gcagucuugg gcuggauugu gagcccagga cuggauugga cuucagcgau     1140

cucuauuacc ugaccaugaa uaacaaacau ugguuagugc acaaggaaug guuucacgac     1200

auuccucugc cauggcaugc uggcgccgau acagguacuc cgcacuggaa uaacaaagag     1260

gcucucgucg aguucaagga cgcccaugca aaacggcaga ccguggucgu gcuugggucc     1320

caggaaggcg ccguucacac agcucuggcg ggagcauugg aagccgagau ggauggcgcu     1380

aaggggcgac uauuuucugg acaucugaag ugccgccuca aaauggacaa gcugagacuu     1440

aaagguguga gcuauucacu guguaccgcc gcauucacuu uuacgaaggu acccgccgag     1500

acauugcacg gcaccgucac uguggaaguu caguacgcug ggaccgacgg accuugcaaa     1560

aucccagugc aaauggccgu cgauaugcag acacucaccc ccgugggcag gcugaucacu     1620

gcaaauccug uaauuacaga gaguacggaa aacuccaaga ugauguuaga gcuggaccca     1680

cccuucggug auagcuacau agugaucggg guuggcgaca agaaaauuac ccaccauugg     1740

caccggucug gaucaacuau cggcaaggcg uucgaagcua caguccgcgg ggccaaaaga     1800

auggcagugc uaggagauac cgccugggac uuugguagug ugggcggggu cuucaauagc     1860

cucggaaagg gcauacauca gauuuuuggu gcugccuuca aaucccuguu ugggggcaug     1920

ucuugguucu cgcaaauccu uaucggaaca uugcugguuu ggcucggccu gaacacuaag     1980

aacgggagca uuucccuuac cugucuggca uugggaggcg ugaugaucuu ucucucaacg     2040

gcugugagug cc                                                         2052


<210>  9808
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9808
auggacugga ccuggauucu guuccucgug gccgcugcaa caagagucca cagcguuacu       60

aggcggggcu ccgccuacua uauguaccuu gaucgcaacg acgcggggga ggcuauuucu      120

uuucccacga cccugggaau gaauaagugc uauauacaga ucauggauuu gggucauaug      180

ugugacgcca caaugucaua cgaaugcccu auguuagaug agggcgugga accagacgau      240

guagacuguu ggugcaacac uaccaguaca uggguggucu augggaccug ucaccauaaa      300

aagggagagg cacgacguag cagaagggcc gugacucugc cgucccacuc uacacggaaa      360

cuacaaaccc gcucgcagac guggcucgaa ucaagagagu acacuaagca ucugauuagg      420

guugagaauu ggaucuuccg gaaccccggc uuugcucuug cagccgcugc cauugcgugg      480

cuguugggua gcaguaccuc ccagaaagug aucuaucucg ucaugauacu gcugaucgca      540

cccgccuacu cuauucgcug caucggggug agcaaucgag auuucguaga aggaaugucc      600

ggcggcacau ggguugacgu gguccucgag cacgggggau gugugacugu gauggcucag      660

gauaagccaa ccgucgacau ugaauuaguu acaaccacgg ugucaaacau ggccgaggua      720

agaaguuacu gcuaugaggc aagcaucucu gauauggcuu ccgacucaag gugucccacu      780

caaggugaag ccuaccugga caagcagagc gauacacagu augugugcaa acggacccuu      840

gucgaccgug gcugggggaa uggauguggc uuguuuggua aggggagucu ggugacuugc      900

gcgaaauucg ccugcucuaa gaaaaugaca ggaaagucca uacagccuga gaaccuagaa      960

uaccgcauua ugcucagcgu ucacggcucg caacauucug gcaugaucgu gaaugauacc     1020

gggcacgaga cggacgaaaa cagagcaaag gucgagauua ccccaaauuc ccccagggcu     1080

gaggccacac ugggagguuu uggcucacuu gggcuggauu gugaaccucg gacuggauug     1140

gacuucagug aucucuauua ccugaccaug aacaauaaac auugguuagu gcacaaggag     1200

ugguuucacg acaucccacu gccguggcau gcuggcgccg auacagguac uccccacugg     1260

aacaauaaag aagcacucgu agaguucaag gacgcccaug cuaaacgaca gaccguugug     1320

guccuuggga gccaggaagg cgcggugcac acagcacugg ccggagcuuu ggaggccgag     1380

auggauggcg caaaggggcg ccuauccucu ggacaucuga aaugcagacu caagauggac     1440

aagcugaggc uuaaaggugu cagcuauuca cuguguaccg ccgcuuuuac uuucacgaag     1500

aucccugccg aaacauugca cggcaccgug acuguugagg ugcaguacgc agggaccgac     1560

ggaccaugca aaguacccgc gcaaauggcu gucgauaugc agacacucac cccugugggc     1620

cggcugauua cugccaaccc aguuauaaca gaaaguacgg agaacuccaa gaugauguua     1680

gaacuggacc cuccguuugg ugauagcuac aucgugauug gggucggcga gaaaaagauc     1740

acccaccauu ggcaccgcuc uggaucaacu auaggcaagg cauucgaggc cacagugaga     1800

ggggcuaaaa ggauggccgu acuaggagac accgcauggg auuucgguag ugugggcggg     1860

gcucucaaua gccugggaaa gggcauucau cagaucuuug gugccgcguu caaaucccuu     1920

uuugggggca ugucuugguu cucgcaaauc uugauuggaa cacugcucau guggcugggc     1980

cuuaacacua agaaugggag caucucccug auguguuugg cccucggagg cguucugauc     2040

uuuuuaucaa ccgcugucag ugca                                            2064


<210>  9809
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9809
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cuguuaccag gcgggguuca gccuacuaua uguaccuuga ccgcaacgau      120

gcaggcgagg ccauaaguuu ccccacaacu cuggggauga auaaauguua uauccagauu      180

auggacuugg gacacaugug cgaugcuacg augagcuacg aauguccuau guuagacgag      240

ggcguggaac cagaugacgu agauugcugg uguaacacca cauccacuug gguggucuau      300

gguaccugcc aucacaagaa aggggaggca cgacguucua gaagggccgu gacacugccg      360

ucgcauucaa cccggaagcu acaaacucgc agccagacau ggcucgaaag uagagaguac      420

accaaacacc ugaucagggu ugagaauugg auuuuucgga accccggauu cgcucuugcc      480

gcggcagcca ucgcuuggcu guugggcucc ucuacgagcc agaaggugau auaucucguc      540

augauccugc ugauugcccc ugcauacucc auccgcugua uuggcguguc aaaucgagac      600

uuuguagaag ggaugagugg agguacuugg guugauaucg ugcucgagca uggcgggugc      660

gucaccguga uggcucagga caagccaaca guggauauag aauuagucac uaccacaguu      720

agcaacaugg ccgaggugag aucuuacugu uaugaggcgu ccauuucaga cauggccagc      780

gauaguaggu gccccaccca aggagaagca uaccuggaca aacagucuga cacgcaguau      840

guauguaagc ggacucuugu ggaucguggc ugggguaaug ggugcggauu guucggcaaa      900

ggcucccugg ucacaugcgc uaaguuugcc uguagcaaaa agaugaccgg gaagucgauc      960

cagccugaga accuagaaua ccgcauuaug cucucugugc acggauccca acacucaggu     1020

augaucguua augacacugg ccaugagaca gaugaaaaca gagcuaaagu ggagaucacc     1080

ccaaauaguc ccagggccga ggcaacgcuu gggggauucg gcagccuggg ucuggacugc     1140

gaaccucgga ccggguugga uuuuuccgac cucuauuacc ugacaaugaa caauaagcac     1200

ugguuagucc auaaagagug guuccacgau auuccacugc cguggcacgc cggcgcugac     1260

acuggaacac cccauuggaa caauaaggaa gcgcucgugg aguuuaaaga ugcacacgcc     1320

aagcgacaga ccguaguugu gcuuggcucu caggaagggg cuguccauac ugcccuggca     1380

ggagccuugg aggcugagau ggacggugcc aaaggccgcc uaagcucagg gcaccugaag     1440

uguagacuca agauggauaa acugaggcuu aagggaguga guuauucccu gugcaccgca     1500

gcguucacau uuaccaaaau accugcugaa acuuugcaug gcacggucac aguggagguu     1560

caguacgccg guaccgacgg gccauguaag gugcccgcac aaauggccgu agacaugcag     1620

acucucaccc cugucggccg gcugaucaca gcuaacccag ugauuaccga aagcacugag     1680

aacucuaaaa ugauguuaga acuggauccc ccguucggag acucauacau cguuauaggc     1740

gugggggaga agaagauuac acaccacugg caucgcagug gaagcacgau cgguaaagcc     1800

uuugaggcaa ccgucagagg cgcuaagagg auggccgugc uaggggauac ugcgugggac     1860

uucggauccg uaggcggugc ccucaauucu cuggggaaag gcauccacca gauuuucgga     1920

gcugcauuua agucgcuuuu cggcgggaug agcugguuuu cccaaaucuu gauuggaaca     1980

cugcucaugu ggcugggccu uaacaccaaa aaugguucaa uaagucugau gugcuuggcc     2040

cucgggggag ugcugaucuu cuuaucuaca gcuguuagcg cc                        2082


<210>  9810
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9810
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cuguuaccag gcgggguuca gccuacuaua uguaccuuga ccgcaacgau      120

gcaggcgagg ccauaaguuu ccccacaacu cuggggauga auaaauguua uauccagauu      180

auggacuugg gacacacgug cgaugcuacc augagcuacg aauguccuau guuagacgag      240

ggcguggaac cagaugacgu agauugcugg uguaacacaa cuuccaccug gguggucuau      300

gguacaugcc aucacaagaa aggggaggca cgacguucua gaagggccgu gacccugccg      360

ucgcauucaa cucggaagcu acaaacacgc agccagaccu ggcucgaaag uagagaguac      420

acgaaacacc ugaucagggu ugagaauugg auuuuucgga accccggauu cgcucuugcc      480

gcggcagcca ucgcuuggcu guugggcucc ucuacuagcc agaaggugau auaucucguc      540

augauccugc ugauugcccc ugcauacucc auccgcugua uuggcguguc aaaucgagac      600

uuuguagaag ggaugagugg agguaccugg guugaugugg uccucgagca uggcgggugc      660

gugacaguga uggcucagga caagccaacu gucgauaucg aauuaguuac cacaaccgug      720

agcaacaugg ccgagguaag aucuuacugu uaugaggcgu ccauaucaga cauggccagc      780

gauaguaggu gccccacgca aggagaagca uaccuggaca aacagucuga cacucaguau      840

guguguaagc ggacacuugu cgaucguggc ugggguaaug ggugcggauu guucggcaaa      900

ggcucccugg ugaccugcgc uaaguuugcc uguagcaaaa agaugacugg gaagucgauu      960

cagccugaga accuagaaua ccgcaucaug cucucuguuc acggauccca acacucaggu     1020

augauuguga augacacagg ccaugagacc gaugaaaaca gagcuaaagu cgagaucacg     1080

ccaaauaguc ccagggccga ggcaacccuu gggggauucg gcagccuggg ucuggacugc     1140

gaaccucgga caggguugga uuuuuccgac cucuauuacc ugacuaugaa caauaagcac     1200

ugguuagugc auaaagagug guuccacgau aucccacugc cguggcacgc cggcgcugac     1260

accggaacac cccauuggaa caauaaggaa gcgcucguag aguuuaaaga ugcacacgcc     1320

aagcgacaga cuguuguggu ccuuggcucu caggaagggg cugugcauac cgcccuggca     1380

ggagccuugg aggcugagau ggacggugcc aaaggccgcc uaagcucagg gcaccugaag     1440

uguagacuca agauggauaa acugaggcuu aagggaguca guuauucccu gugcacagca     1500

gcguucaccu uuacuaaaau uccugcugaa acguugcaug gcacagugac cguugaggug     1560

caguacgccg guacugacgg gccauguaag guacccgcac aaauggccgu cgacaugcag     1620

acccucacac cugugggccg gcugauaacc gcuaacccag uuaucacuga aagcacagag     1680

aacucuaaaa ugauguuaga acuggauccc ccguucggag acucauacau ugugaucggc     1740

gucggggaga agaagauaac gcaccacugg caucgcagug gaagcaccau ugguaaagcc     1800

uuugaggcaa cugugagagg cgcuaagagg auggccguac uaggggauac agcgugggac     1860

uucggauccg ugggcggugc ccucaauucu cuggggaaag gcauccacca gaucuucgga     1920

gcugcauuua agucgcuuuu cggcgggaug agcugguuuu cccaaauuuu gaucggaacc     1980

cugcucaugu ggcugggccu uaacgccaaa aaugguucaa uuagucugau gugcuuggcu     2040

cucgggggag uucugauauu cuuaucuaca gccgucagcg ca                        2082


<210>  9811
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9811
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cuauaaccag gcgggguuca gccuacuaua uguaccuuga ccgcagugau      120

gcaggcaaag ccaucagcuu cgcuacaacu cuggggguua acaaguguca cgugcagauu      180

auggacuugg gacauaugug cgaugcaacg auguccuaug agugucccau guuagacgaa      240

ggcguagagc cugaugacgu ggauugcugg uguaauacca caucuacuug ggucguguac      300

gguaccugcc accauaaaaa gggggaagcc cgacguucga gaagggcugu uacacugcca      360

ucacacagca cccggaaacu acaaacucgc agucagacau ggcucgaguc cagagaauau      420

accaagcauc ugaucaaggu ggagaacugg auuuuuagga auccgggauu cgcccuuguc      480

gcgguggcaa ucgccuggcu guugggcucu agcacguccc agaaaguaau auaccucguu      540

augauccugc ugauugcucc cgccuauuca auccggugua uuggcgugag uaaccgcgac      600

uuugucgagg ggaugagcgg agguacuugg guggaugugg uccucgaaca cggcgggugc      660

guuaccguga uggcacagga caagccuaca guagauaucg aguuagugac uaccacaguc      720

ucuaauaugg cugaagugcg auccuacugu uacgaggccu caauaagcga cauggcgagu      780

gauucuagau gcccaaccca aggagaggcc uaucuggaca aacaguccga cacgcaguac      840

guuuguaaga ggacucuugu ggaucggggc ugggguaacg ggugcggauu guucggcaaa      900

ggcagccugg ucacaugcgc aaaguuuacc uguucgaaga aaaugacugg gaagucuauu      960

cagcccgaaa aucuagagua ucguaucaug cucuccgugc acggaucaca acauaguggu     1020

augauuggcu acgaaacaga cgaggaucgc gcuaaaguag aaguuacccc uaacagccca     1080

agagccgagg cuacgcuggg aggguucggc ucccuugguc uggacugcga gcccaggacc     1140

ggguuggauu uuucugaccu cuauuaccug acaaugaaua acaagcacug guuagugcau     1200

aaagaauggu uccacgauau cccucugcca uggcacgccg gcgcagacac uggaaccccg     1260

cauuggaaua acaaggaggc ccucgucgaa uuuaaagaug cucacgcgaa gcggcagaca     1320

guggucgugc uuggcagcca ggagggggca guucauacug cccuggcugg agccuuggaa     1380

gcagagaugg acggugccaa gggccgacua uucucagggc accugaaaug ucgccucaag     1440

auggauaaac ugagacuuaa gggagugagu uauucccugu gcaccgcugc cuuuacauuc     1500

accaaaguac ccgcagagac uuugcauggc acggucacag uggaaguuca guacgcgggu     1560

accgacgggc cuuguaagau cccagugcaa auggcugucg acaugcagac ucucaccccc     1620

gugggcaggc ugauuacagc caauccugua auaaccgaga gcacugaaaa cucuaagaug     1680

auguuagagc uggauccacc cuuuggagac ucauacaucg ugauuggcgu uggggauaaa     1740

aagaucacac accacuggca ucggagugga agcacgauag guaaagcauu cgaagccacc     1800

guccgcggcg cuaagagaau ggccgugcua ggggacacug caugggauuu cggauccgug     1860

ggcggugucu uuaauucucu cgggaaaggc auucaccaga ucuucggagc ugccuuuaag     1920

ucgcuguucg gcgggaugag cugguuuucc caaauccuua uuggaacauu gcugguuugg     1980

cucggccuga acaccaagaa cgguucaauc agucuuacau gccuggcgcu cgggggagug     2040

augauuuucu ugucuacugc cgugagcgcu                                      2070


<210>  9812
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9812
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cuguuaccag gcgggguuca gccuacuaua uguaccuuga ccgcaacgau      120

gcaggcgagg ccauaaguuu ccccacaacu cuggggauga auaaauguua uauccagauu      180

auggacuugg gacacaugug cgaugcuacg augagcuacg aauguccuau guuagacgag      240

ggcguggaac cagaugacgu agauugcugg uguaacacca cauccacuug gguggucuau      300

gguaccugcc aucacaagaa aggggaggca cgacguucua gaagggccgu gacacugccg      360

ucgcauucaa cccggaagcu acaaacucgc agccagacau ggcucgaaag uagagaguac      420

accaaacacc ugaucagggu ugagaauugg auuuuucgga accccggauu cgcucuugcc      480

gcggcagcca ucgcuuggcu guugggcucc ucuacgagcc agaaggugau auaucucguc      540

augauccugc ugauugcccc ugcauacucc auccgcugua uuggcguguc aaaucgagac      600

uuuguagaag ggaugagugg agguacuugg guugaugugg uccucgagca uggcgggugc      660

gugaccguga uggcucagga caagccaaca gucgauaucg aauuaguuac uaccacagug      720

agcaacaugg ccgagguaag aucuuacugu uaugaggcgu ccauaucaga cauggccagc      780

gauaguaggu gccccaccca aggagaagca uaccuggaca aacagucuga cacgcaguau      840

guguguaagc ggacucuugu cgaucguggc ugggguaaug ggugcggauu guucggcaaa      900

ggcucccugg ugacaugcgc uaaguuugcc uguagcaaaa agaugaccgg gaagucgauu      960

cagccugaga accuagaaua ccgcaucaug cucucuguuc acggauccca acacucaggu     1020

augauuguga augacacugg ccaugagaca gaugaaaaca gagcuaaagu cgagaucacc     1080

ccaaauaguc ccagggccga ggcaacgcuu gggggauucg gcagccuggg ucuggacugc     1140

gaaccucgga ccggguugga uuuuuccgac cucuauuacc ugacaaugaa caauaagcac     1200

ugguuagugc auaaagagug guuccacgau aucccacugc cguggcacgc cggcgcugac     1260

acuggaacac cccauuggaa caauaaggaa gcgcucguag aguuuaaaga ugcacacgcc     1320

aagcgacaga ccguuguggu ccuuggcucu caggaagggg cugugcauac ugcccuggca     1380

ggagccuugg aggcugagau ggacggugcc aaaggccgcc uaagcucagg gcaccugaag     1440

uguagacuca agauggauaa acugaggcuu aagggaguca guuauucccu gugcaccgca     1500

gcguucacau uuaccaaaau uccugcugaa acuuugcaug gcacggugac aguugaggug     1560

caguacgccg guaccgacgg gccauguaag guacccgcac aaauggccgu cgacaugcag     1620

acucucaccc cugugggccg gcugauaaca gcuaacccag uuaucaccga aagcacugag     1680

aacucuaaaa ugauguuaga acuggauccc ccguucggag acucauacau ugugaucggc     1740

gucggggaga agaagauaac acaccacugg caucgcagug gaagcacgau ugguaaagcc     1800

uuugaggcaa ccgugagagg cgcuaagagg auggccguac uaggggauac ugcgugggac     1860

uucggauccg ugggcggugc ccucaauucu cuggggaaag gcauccacca gaucuucgga     1920

gcugcauuua agucgcuuuu cggcgggaug agcugguuuu cccaaauuuu gaucggaaca     1980

cugcucaugu ggcugggccu uaacaccaaa aaugguucaa uuagucugau gugcuuggcc     2040

cucgggggag uucugauauu cuuaucuaca gcugucagcg cc                        2082


<210>  9813
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9813
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgugac cagaagggga ucagcguacu auauguaccu ugaccggaac      120

gaugcuggug aagccaucag uuucccuaca acucugggca ugaauaagug cuauauucaa      180

aucauggacu uggggcacau gugugaugca acgaugagcu acgagugccc aauguuagac      240

gaaggaguag agccggauga cguggauugu uggugcaaca ccacauccac uugggucgug      300

uauggcaccu gucaucacaa aaagggugaa gcccgccgau cucguagagc uguuacacug      360

cccucgcauu caaccaggaa acuacagacu cggagccaga cauggcucga gagucgcgag      420

uacaccaagc accugauuag aguggaaaau uggaucuuua ggaacccugg guucgcacuu      480

gccgcugccg cgauagcaug gcuguuggga uccucuacga gccagaaagu caucuaucuc      540

gugaugauuc ugcugaucgc cccagcuuac uccauucggu gcaucggcgu aucaaaucgc      600

gacuuuguug agggcaugag ugggggaacu uggguggaua uaguccucga acaugguggc      660

ugugugaccg ugauggccca agacaagccc acagucgaua uugaguuagu uacuaccaca      720

gugagcaaca uggcagaggu acgaucuuac ugcuaugagg cuuccaucuc agacauggcc      780

agcgauagua gauguccuac ccagggggaa gcguaccugg acaagcaguc ugacacgcag      840

uaugugugca aaaggacucu ugucgaucgg ggauggggca augguugugg guuguucgga      900

aagggcuccc uggugacaug cgccaaauuu gcaugcagca agaaaaugac cggcaagucg      960

auucaaccag aaaaccuaga guaccguauc augcucucug uucacggguc ccagcacuca     1020

ggaaugaucg ugaaugacac uggucaugaa acagaugaga accgcgcuaa ggucgagauu     1080

acccccaaua guccuagagc cgaagcuacg cugggcgggu ucggaagccu uggccuggac     1140

ugugagccaa ggaccgguuu ggauuuuucc gaccucuauu accugacaau gaacaauaaa     1200

cacugguuag ugcauaagga augguuccac gauauaccgc ugcccuggca cgccggggca     1260

gacacuggca ccccucauug gaacaauaaa gaggcccucg uagaauuuaa ggaugcucac     1320

gcgaaacggc agacaguugu gguccuugga ucucaggagg gcgcagugca uacugcccug     1380

gcuggggccu uggaggcaga aauggacgga gccaaggguc gacuaagcuc aggccaccug     1440

aaaugccgcc ucaagaugga uaagcugaga cuuaaagggg ucaguuauuc ccuguguacc     1500

gcugccuuca cauuuaccaa gaucccagca gagacuuugc auggaacggu gacaguugaa     1560

gugcaauacg cgggcaccga cggucccugc aaaguaccug cucagauggc cgucgacaug     1620

cagacucuca ccccaguggg gaggcugauu acagcaaacc ccguuaucac cgagagcacu     1680

gaaaacucua agaugauguu agagcuggau ccgccuuucg gcgacucaua cauagugauu     1740

ggagucggcg agaaaaagau cacacaccac uggcaucgga gugggagcac gcuaggaaag     1800

gccuucucca ccacucucaa aggugcucaa cgccuggccg cacuuggcga uacagcuugg     1860

gacuuugggu cuaucggagg cguguucaau ucgauuggua gagccguaca ccagguguuu     1920

gggggcgcgu ucaggaccuu guuuggaggc augagcugga ucacacaggg gcugauggga     1980

gcccuccugc uuuggauggg cguuaacgcu cgugaucggu ccauugcacu ggccuucuug     2040

gcuacuggug ggguccucgu guuucuggcc accaaugugc augca                     2085


<210>  9814
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9814
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgugac cagaagggga ucagcguacu auauguaccu ugaccggaac      120

gaugcuggug aagccaucag uuucccuaca acucugggca ugaauaagug cuauauucaa      180

aucauggacu uggggcacac gugugaugca accaugagcu acgagugccc aauguuagac      240

gaaggaguag agccggauga cguggauugu uggugcaaca caacuuccac cugggucgug      300

uauggcacau gucaucacaa aaagggugaa gcccgccgau cucguagagc uguuacccug      360

cccucgcauu caacuaggaa acuacagaca cggagccaga ccuggcucga gagucgcgag      420

uacacgaagc accugauuag aguggaaaau uggaucuuua ggaacccugg guucgcacuu      480

gccgcugccg cgauagcaug gcuguuggga uccucuacua gccagaaagu caucuaucuc      540

gugaugauuc ugcugaucgc cccagcuuac uccauucggu gcaucggcgu aucaaaucgc      600

gacuuuguug agggcaugag ugggggaacc uggguggaug ucgugcucga acaugguggc      660

ugugugacag ucauggccca agacaagccc acuguugaua uagaguuagu gaccacaacc      720

guaagcaaca uggcagaggu gcgaucuuac ugcuaugagg cuuccauuuc agacauggcc      780

agcgauagua gauguccuac gcagggggaa gcguaccugg acaagcaguc ugacacucag      840

uaugucugca aaaggacacu uguggaucgg ggauggggca augguugugg guuguucgga      900

aagggcuccc ugguuaccug cgccaaauuu gcaugcagca agaaaaugac uggcaagucg      960

auccaaccag aaaaccuaga guaccguauu augcucucug ugcacggguc ccagcacuca     1020

ggaaugaucg ucaaugacac aggucaugaa accgaugaga accgcgcuaa gguggagauc     1080

acgcccaaua guccuagagc cgaagcuacc cugggcgggu ucggaagccu uggccuggac     1140

ugugagccaa ggacagguuu ggauuuuucc gaccucuauu accugacuau gaacaauaaa     1200

cacugguuag uacauaagga augguuccac gauauuccgc ugcccuggca cgccggggca     1260

gacaccggca caccucauug gaacaauaaa gaggcccucg uugaauuuaa ggaugcucac     1320

gcgaaacggc agacuguggu cgugcuugga ucucaggagg gcgcagucca uaccgcccug     1380

gcuggggccu uggaggcaga aauggacgga gccaaggguc gacuaagcuc aggccaccug     1440

aaaugccgcc ucaagaugga uaagcugaga cuuaaagggg ugaguuauuc ccuguguaca     1500

gcugccuuca ccuuuacuaa gauaccagca gagacguugc auggaacagu uaccguggaa     1560

guacaauacg cgggcacuga cggucccugc aaagucccug cucagauggc cguggacaug     1620

cagacccuca caccaguugg gaggcugauc accgcaaacc ccgugauuac ugagagcaca     1680

gaaaacucua agaugauguu agagcuggau ccgccuuucg gcgacucaua caucgucaua     1740

ggagugggcg agaaaaagau uacgcaccac uggcaucgga gugggagcac ccuaggaaag     1800

gccuucucca cuacacucaa aggugcucaa cgccuggccg cacuuggcga uaccgcuugg     1860

gacuuugggu cuaucggagg cguauucaau ucgaucggua gagccgugca ccagguuuuu     1920

gggggcgcgu ucaggacauu guuuggaggc augagcugga uuacucaggg gcugauggga     1980

gcccuccugc uuuggauggg cgucaacgcu cgugaucggu ccaucgcacu ggccuucuug     2040

gcuaccggug gggugcucgu guuucuggcc acgaaugucc augca                     2085


<210>  9815
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9815
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccaucac cagaagggga ucagcguacu auauguaccu ugaccggagu      120

gaugcuggua aggccauuag cuucgcaaca acucugggcg ugaacaaaug ccacguacaa      180

aucauggacu uggggcauau gugugaugcc acgauguccu augaaugccc uauguuagac      240

gagggagugg aaccagauga cgucgauugu uggugcaaua ccacaucuac uugggugguu      300

uacggcaccu gucaccauaa gaaaggugag gcucgccgau cgcguagagc agugacacug      360

ccgucacaca gcaccaggaa gcuacagacu cggagucaga cauggcucga aucccgcgag      420

uauaccaaac aucugauuaa ggucgagaac uggaucuuua gaaaucccgg guucgcccuu      480

guggcuguag ccauagcgug gcuguuggga ucuagcacgu cccagaaggu uaucuaccuc      540

gugaugauuc ugcugaucgc acccgccuau ucaauuaggu gcaucggcgu caguaaccgg      600

gacuuugugg aaggcaugag cgggggaacu uggguggaug ucguucucga gcacgguggc      660

ugugugaccg uaauggcuca agacaaacca acaguggaua uagaauuagu cacuaccaca      720

gugucuaaua uggccgaggu ucgcuccuac ugcuacgagg caucaauuag cgacauggcu      780

agugauucuc gaugucccac ccagggggaa gccuaucugg acaagcaguc cgacacgcag      840

uacgugugca aaagaacucu ugucgauagg ggauggggca acgguugugg guuguucgga      900

aagggcagcc uggugacaug cgcgaaauuu accugcucga agaagaugac uggcaaaucu      960

auccaaccug agaaucuaga auaucggauu augcucuccg uacacggguc acagcauagu     1020

ggaaugaucg guuacgagac agacgaagau cgugccaagg uugaggugac cccaaacagc     1080

ccccgcgcag aggcuacgcu gggcggguuc ggaucccuug gccuggacug ugaaccuaga     1140

accgguuugg auuuuucuga ccucuauuac cugacaauga auaacaaaca cugguuaguc     1200

cauaaggagu gguuccacga uaucccacug ccguggcacg ccggggcuga cacuggcacc     1260

ccucauugga auaacaaaga agcccucgug gaguuuaagg augcacacgc caaaaggcag     1320

acagucgugg uucuuggaag ccaggaaggc gcugugcaua cugcgcuggc aggggccuug     1380

gaggcugaga uggacggagc caagggucgg cuauucucag gccaccugaa gugccgacuc     1440

aaaauggaua agcugcgccu uaaaggggua aguuauuccc uguguaccgc agccuuuaca     1500

uucaccaagg ucccugcuga aacuuugcau ggaacgguga caguugaggu gcaauacgcc     1560

ggcaccgacg guccaugcaa aauucccguc cagauggcag uggacaugca gacucucacc     1620

ccuguaggga gacugauaac agcgaaucca gugaucaccg aaagcacuga gaacucuaag     1680

augauguuag aacuggaucc cccguucggc gacucauaca uuguuaucgg agucggcgau     1740

aagaaaauaa cacaccacug gcauaggagu gggagcacgc uaggaaaggc uuuuuccacc     1800

acucucaaag gugcccaacg gcuggcagcc cuuggcgaca cagcuuggga uuucgggucu     1860

auuggaggcg uguuuaauuc gaucggucgc gccgugcacc aggucuucgg gggcgcauuu     1920

agaaccuugu ucggaggcau gagcuggauc acacaggggc ugaugggagc ucuccugcuu     1980

uggaugggcg uuaacgccag ggaccguucc auugcgcugg ccuuuuuggc uacugguggg     2040

gugcucgugu uccuggcaac caacguacau gcc                                  2073


<210>  9816
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9816
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgugac cagaagggga ucagcguacu auauguaccu ugaccggaac      120

gaugcuggug aagccaucag uuucccuaca acucugggca ugaauaagug cuauauucaa      180

aucauggacu uggggcacau gugugaugca acgaugagcu acgagugccc aauguuagac      240

gaaggaguag agccggauga cguggauugu uggugcaaca ccacauccac uugggucgug      300

uauggcaccu gucaucacaa aaagggugaa gcccgccgau cucguagagc uguuacacug      360

cccucgcauu caaccaggaa acuacagacu cggagccaga cauggcucga gagucgcgag      420

uacaccaagc accugauuag aguggaaaau uggaucuuua ggaacccugg guucgcacuu      480

gccgcugccg cgauagcaug gcuguuggga uccucuacga gccagaaagu caucuaucuc      540

gugaugauuc ugcugaucgc cccagcuuac uccauucggu gcaucggcgu aucaaaucgc      600

gacuuuguug agggcaugag ugggggaacu uggguggaug ucgugcucga acaugguggc      660

ugugugaccg ucauggccca agacaagccc acaguugaua uagaguuagu gacuaccaca      720

guaagcaaca uggcagaggu gcgaucuuac ugcuaugagg cuuccauuuc agacauggcc      780

agcgauagua gauguccuac ccagggggaa gcguaccugg acaagcaguc ugacacgcag      840

uaugucugca aaaggacucu uguggaucgg ggauggggca augguugugg guuguucgga      900

aagggcuccc ugguuacaug cgccaaauuu gcaugcagca agaaaaugac cggcaagucg      960

auccaaccag aaaaccuaga guaccguauu augcucucug ugcacggguc ccagcacuca     1020

ggaaugaucg ucaaugacac uggucaugaa acagaugaga accgcgcuaa gguggagauc     1080

acccccaaua guccuagagc cgaagcuacg cugggcgggu ucggaagccu uggccuggac     1140

ugugagccaa ggaccgguuu ggauuuuucc gaccucuauu accugacaau gaacaauaaa     1200

cacugguuag uacauaagga augguuccac gauauuccgc ugcccuggca cgccggggca     1260

gacacuggca ccccucauug gaacaauaaa gaggcccucg uugaauuuaa ggaugcucac     1320

gcgaaacggc agacaguggu cgugcuugga ucucaggagg gcgcagucca uacugcccug     1380

gcuggggccu uggaggcaga aauggacgga gccaaggguc gacuaagcuc aggccaccug     1440

aaaugccgcc ucaagaugga uaagcugaga cuuaaagggg ugaguuauuc ccuguguacc     1500

gcugccuuca cauuuaccaa gauaccagca gagacuuugc auggaacggu uacaguggaa     1560

guacaauacg cgggcaccga cggucccugc aaagucccug cucagauggc cguggacaug     1620

cagacucuca ccccaguugg gaggcugauc acagcaaacc ccgugauuac cgagagcacu     1680

gaaaacucua agaugauguu agagcuggau ccgccuuucg gcgacucaua caucgucaua     1740

ggagugggcg agaaaaagau uacacaccac uggcaucgga gugggagcac gcuaggaaag     1800

gccuucucca ccacucucaa aggugcucaa cgccuggccg cacuuggcga uacagcuugg     1860

gacuuugggu cuaucggagg cguauucaau ucgaucggua gagccgugca ccagguuuuu     1920

gggggcgcgu ucaggaccuu guuuggaggc augagcugga uuacacaggg gcugauggga     1980

gcccuccugc uuuggauggg cgucaacgcu cgugaucggu ccaucgcacu ggccuucuug     2040

gcuacuggug gggugcucgu guuucuggcc accaaugucc augca                     2085


<210>  9817
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9817
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcguuacu       60

aggcggggcu ccgccuacua uauguaccuu gaucgcaacg acgcggggga ggcuauuucu      120

uuucccacga cccugggaau gaauaagugc uauauacaga ucauggauuu gggucauaug      180

ugugacgcca caaugucaua cgaaugcccu auguuagaug agggcgugga accagacgau      240

guagacuguu ggugcaacac uaccaguaca uggguggucu augggaccug ucaccauaaa      300

aagggagagg cacgacguag cagaagggcc gugacucugc cgucccacuc uacacggaaa      360

cuacaaaccc gcucgcagac guggcucgaa ucaagagagu acacuaagca ucugauuagg      420

guugagaauu ggaucuuccg gaaccccggc uuugcucuug cagccgcugc cauugcgugg      480

cuguugggua gcaguaccuc ccagaaagug aucuaucucg ucaugauacu gcugaucgca      540

cccgccuacu cuauucgcug caucggggug agcaaucgag auuucguaga aggaaugucc      600

ggcggcacau ggguugacau ugugcucgag cacgggggau gugucacugu gauggcucag      660

gauaagccaa ccguggacau cgaauuaguc acaaccacgg uuucaaacau ggccgaggug      720

agaaguuacu gcuaugaggc aagcauaucu gauauggcuu ccgacucaag gugucccacu      780

caaggugaag ccuaccugga caagcagagc gauacacagu auguaugcaa acggacccuu      840

guggaccgug gcugggggaa uggauguggc uuguuuggua aggggagucu ggucacuugc      900

gcgaaauucg ccugcucuaa gaaaaugaca ggaaagucca uucagccuga gaaccuagaa      960

uaccgcauca ugcucagcgu gcacggcucg caacauucug gcaugauugu uaaugauacc     1020

gggcacgaga cggacgaaaa cagagcaaag guggagauca ccccaaauuc ccccagggcu     1080

gaggccacac ugggagguuu uggcucacuu gggcuggauu gugaaccucg gacuggauug     1140

gacuucagug aucucuauua ccugaccaug aacaauaaac auugguuagu ccacaaggag     1200

ugguuucacg acaucccacu gccguggcau gcuggcgccg auacagguac uccccacugg     1260

aacaauaaag aagcacucgu ggaguucaag gacgcccaug cuaaacgaca gaccguaguu     1320

gugcuuggga gccaggaagg cgcgguccac acagcacugg ccggagcuuu ggaggccgag     1380

auggauggcg caaaggggcg ccuauccucu ggacaucuga aaugcagacu caagauggac     1440

aagcugaggc uuaaaggugu gagcuauuca cuguguaccg ccgcuuuuac uuucacgaag     1500

auuccugccg aaacauugca cggcaccguc acuguggagg uucaguacgc agggaccgac     1560

ggaccaugca aagugcccgc gcaaauggcu guagauaugc agacacucac cccugucggc     1620

cggcugauaa cugccaaccc agugaucaca gaaaguacgg agaacuccaa gaugauguua     1680

gaacuggacc cuccguuugg ugauagcuac auuguuaucg gggugggcga gaaaaagaua     1740

acccaccauu ggcaccgcuc uggaucaacu cuaggcaagg cauucaguac aacccucaaa     1800

ggggcccaga gacuggcugc ccuuggagac acagcauggg auuucgguag cauuggcggg     1860

gucuuuaauu ccaucggaag ggcugugcau caaguauucg gcggugccuu ucguacuuug     1920

uucgggggca ugucuuggau cacccaggga cugaugggcg cgcuccugcu uuggaugggg     1980

gugaacgccc gggaccgauc gauugcucug gcauuuuugg ccacgggagg cguucucguc     2040

uuccuggcua ccaaugugca cgcc                                            2064


<210>  9818
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9818
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcguuacu       60

aggcggggcu ccgccuacua uauguaccuu gaucgcaacg acgcggggga ggcuauuucu      120

uuucccacga cccugggaau gaauaagugc uauauacaga ucauggauuu gggucauaca      180

ugugacgcca cuaugucaua cgaaugcccu auguuagaug agggcgugga accagacgau      240

guagacuguu ggugcaacac cacaaguacc uggguggucu augggacuug ucaccauaaa      300

aagggagagg cacgacguag cagaagggcc gugacacugc cgucccacuc uacccggaaa      360

cuacaaacgc gcucgcagac uuggcucgaa ucaagagagu acaccaagca ucugauuagg      420

guugagaauu ggaucuuccg gaaccccggc uuugcucuug cagccgcugc cauugcgugg      480

cuguugggua gcaguacauc ccagaaagug aucuaucucg ucaugauacu gcugaucgca      540

cccgccuacu cuauucgcug caucggggug agcaaucgag auuucguaga aggaaugucc      600

ggcggcacuu ggguugacgu gguccucgag cacgggggau gugugaccgu gauggcucag      660

gauaagccaa cagucgacau ugaauuaguu accacgacug ugucaaacau ggccgaggua      720

agaaguuacu gcuaugaggc aagcaucucu gauauggcuu ccgacucaag gugucccaca      780

caaggugaag ccuaccugga caagcagagc gauacccagu augugugcaa acggacucuu      840

gucgaccgug gcugggggaa uggauguggc uuguuuggua aggggagucu ggugacaugc      900

gcgaaauucg ccugcucuaa gaaaaugacc ggaaagucca uacagccuga gaaccuagaa      960

uaccgcauua ugcucagcgu ucacggcucg caacauucug gcaugaucgu gaaugauacg     1020

gggcacgaga ccgacgaaaa cagagcaaag gucgagauua caccaaauuc ccccagggcu     1080

gaggccacuc ugggagguuu uggcucacuu gggcuggauu gugaaccucg gaccggauug     1140

gacuucagug aucucuauua ccugacaaug aacaauaaac auugguuagu gcacaaggag     1200

ugguuucacg acaucccacu gccguggcau gcuggcgccg auacugguac cccucacugg     1260

aacaauaaag aagcacucgu agaguucaag gacgcccaug cuaaacgaca gacaguugug     1320

guccuuggga gccaggaagg cgcggugcac accgcacugg ccggagcuuu ggaggccgag     1380

auggauggcg caaaggggcg ccuauccucu ggacaucuga aaugcagacu caagauggac     1440

aagcugaggc uuaaaggugu cagcuauuca cuguguacug ccgcuuuuac guucacaaag     1500

aucccugccg aaaccuugca cggcacugug accguugagg ugcaguacgc agggacagac     1560

ggaccaugca aaguacccgc gcaaauggcu gucgauaugc agacccucac uccugugggc     1620

cggcugauua cagccaaccc aguuauaacg gaaaguaccg agaacuccaa gaugauguua     1680

gaacuggauc ccccguuugg ugacagcuac aucgugauug gggucggcga gaaaaagauc     1740

acucaccauu ggcaccgcuc uggaucaaca cuaggcaagg cauucaguac cacacucaaa     1800

ggggcccaga gacuggcugc ccuuggagac acugcauggg auuucgguag cauaggcggg     1860

guguuuaauu ccauuggaag ggcuguacau caaguguucg gcggugccuu ucguaccuug     1920

uucgggggca ugucuuggau cacgcaggga cugaugggcg cgcuccugcu uuggaugggg     1980

guuaacgccc gggaccgauc gaucgcucug gcauuuuugg ccaccggagg cguccucgug     2040

uuccuggcua caaaugugca cgcc                                            2064


<210>  9819
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9819
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcauuacu       60

aggcggggcu ccgccuacua uauguaccuu gaucgcucug acgcggggaa ggcuauauca      120

uuugccacga cccugggagu uaacaaaugc caugugcaga ucauggauuu gggucacaug      180

ugugacgcaa caaugaguua ugagugcccc auguuagaug aaggcguaga gccugacgau      240

guggacuguu ggugcaauac uaccagcaca ugggucgugu acgggaccug ucaucacaag      300

aaaggagaag cccgacguuc cagaagggcu guuacucugc caucucauuc gacacggaag      360

cuacaaaccc gcucacagac guggcucgag agcagagaau auacuaaaca ccugauuaag      420

guggagaacu ggaucuucag gaauccgggc uuugcacuug ucgccguggc uauugccugg      480

cuguugggua guuccaccuc ucagaaggua aucuaccucg uuaugauacu gcugaucgcg      540

cccgcauaua gcauucggug caucggggug uccaaccgcg auuucgucga gggaauguca      600

ggcggcacau ggguggacgu gguccucgaa cacgggggau guguuacugu gauggcccag      660

gauaaaccua ccguagacau ugaguuagug acaaccacgg ucaguaauau ggcugaagug      720

cgaagcuacu gcuacgaggc cucuaucucc gauauggcau cagacagcag auguccaacu      780

caaggugagg cuuaucugga caagcagagu gauacacagu acguuugcaa aaggacccuu      840

guggaccggg gcugggggaa cggauguggc uuguuuggua aggggucucu ggucacuugc      900

gccaaauuca caugcuccaa gaagaugacc ggaaaaagca uacagcccga aaaucuagag      960

uaucguauua ugcucucggu gcauggcucu caacacuccg gcaugaucgg guacgaaacg     1020

gaugaggacc gcgcgaaggu agaaguuacc ccuaacucac caagagccga ggcaacacug     1080

ggagguuuug gcagucuugg gcuggauugu gagcccagga cuggauugga cuucagcgau     1140

cucuauuacc ugaccaugaa uaacaaacau ugguuagugc acaaggaaug guuucacgac     1200

auuccucugc cauggcaugc uggcgccgau acagguacuc cgcacuggaa uaacaaagag     1260

gcucucgucg aguucaagga cgcccaugca aaacggcaga ccguggucgu gcuugggucc     1320

caggaaggcg ccguucacac agcucuggcg ggagcauugg aagccgagau ggauggcgcu     1380

aaggggcgac uauuuucugg acaucugaag ugccgccuca aaauggacaa gcugagacuu     1440

aaagguguga gcuauucacu guguaccgcc gcauucacuu uuacgaaggu acccgccgag     1500

acauugcacg gcaccgucac uguggaaguu caguacgcug ggaccgacgg accuugcaaa     1560

aucccagugc aaauggccgu cgauaugcag acacucaccc ccgugggcag gcugaucacu     1620

gcaaauccug uaauuacaga gaguacggaa aacuccaaga ugauguuaga gcuggaccca     1680

cccuucggug auagcuacau agugaucggg guuggcgaca agaaaauuac ccaccauugg     1740

caccggucug gaucaacucu aggcaaggcg uucaguacaa cccucaaagg ggcucagcgc     1800

cuggccgcac uuggagauac agccugggac uuugguagca ucggcggggu cuucaauucc     1860

auaggcagag cugugcauca aguguuugga ggugccuuca ggacuuuguu ugggggcaug     1920

ucuuggauua cccagggacu gaugggcgca cuccugcuuu ggaugggggu caacgcucgu     1980

gaucggucga ucgcccuggc guucuuggcc acgggaggcg uucucguguu ucuggcuacc     2040

aacgugcacg ca                                                         2052


<210>  9820
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9820
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcguuacu       60

aggcggggcu ccgccuacua uauguaccuu gaucgcaacg acgcggggga ggcuauuucu      120

uuucccacga cccugggaau gaauaagugc uauauacaga ucauggauuu gggucauaug      180

ugugacgcca caaugucaua cgaaugcccu auguuagaug agggcgugga accagacgau      240

guagacuguu ggugcaacac uaccaguaca uggguggucu augggaccug ucaccauaaa      300

aagggagagg cacgacguag cagaagggcc gugacucugc cgucccacuc uacacggaaa      360

cuacaaaccc gcucgcagac guggcucgaa ucaagagagu acacuaagca ucugauuagg      420

guugagaauu ggaucuuccg gaaccccggc uuugcucuug cagccgcugc cauugcgugg      480

cuguugggua gcaguaccuc ccagaaagug aucuaucucg ucaugauacu gcugaucgca      540

cccgccuacu cuauucgcug caucggggug agcaaucgag auuucguaga aggaaugucc      600

ggcggcacau ggguugacgu gguccucgag cacgggggau gugugacugu gauggcucag      660

gauaagccaa ccgucgacau ugaauuaguu acaaccacgg ugucaaacau ggccgaggua      720

agaaguuacu gcuaugaggc aagcaucucu gauauggcuu ccgacucaag gugucccacu      780

caaggugaag ccuaccugga caagcagagc gauacacagu augugugcaa acggacccuu      840

gucgaccgug gcugggggaa uggauguggc uuguuuggua aggggagucu ggugacuugc      900

gcgaaauucg ccugcucuaa gaaaaugaca ggaaagucca uacagccuga gaaccuagaa      960

uaccgcauua ugcucagcgu ucacggcucg caacauucug gcaugaucgu gaaugauacc     1020

gggcacgaga cggacgaaaa cagagcaaag gucgagauua ccccaaauuc ccccagggcu     1080

gaggccacac ugggagguuu uggcucacuu gggcuggauu gugaaccucg gacuggauug     1140

gacuucagug aucucuauua ccugaccaug aacaauaaac auugguuagu gcacaaggag     1200

ugguuucacg acaucccacu gccguggcau gcuggcgccg auacagguac uccccacugg     1260

aacaauaaag aagcacucgu agaguucaag gacgcccaug cuaaacgaca gaccguugug     1320

guccuuggga gccaggaagg cgcggugcac acagcacugg ccggagcuuu ggaggccgag     1380

auggauggcg caaaggggcg ccuauccucu ggacaucuga aaugcagacu caagauggac     1440

aagcugaggc uuaaaggugu cagcuauuca cuguguaccg ccgcuuuuac uuucacgaag     1500

aucccugccg aaacauugca cggcaccgug acuguugagg ugcaguacgc agggaccgac     1560

ggaccaugca aaguacccgc gcaaauggcu gucgauaugc agacacucac cccugugggc     1620

cggcugauua cugccaaccc aguuauaaca gaaaguacgg agaacuccaa gaugauguua     1680

gaacuggacc cuccguuugg ugauagcuac aucgugauug gggucggcga gaaaaagauc     1740

acccaccauu ggcaccgcuc uggaucaacu cuaggcaagg cauucaguac aacccucaaa     1800

ggggcccaga gacuggcugc ccuuggagac acagcauggg auuucgguag cauaggcggg     1860

guguuuaauu ccauuggaag ggcuguacau caaguguucg gcggugccuu ucguacuuug     1920

uucgggggca ugucuuggau cacccaggga cugaugggcg cgcuccugcu uuggaugggg     1980

guuaacgccc gggaccgauc gaucgcucug gcauuuuugg ccacgggagg cguccucgug     2040

uuccuggcua ccaaugugca cgcc                                            2064


<210>  9821
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9821
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cuguuaccag gcgggguuca gccuacuaua uguaccuuga ccgcaacgau      120

gcaggcgagg ccauaaguuu ccccacaacu cuggggauga auaaauguua uauccagauu      180

auggacuugg gacacaugug cgaugcuacg augagcuacg aauguccuau guuagacgag      240

ggcguggaac cagaugacgu agauugcugg uguaacacca cauccacuug gguggucuau      300

gguaccugcc aucacaagaa aggggaggca cgacguucua gaagggccgu gacacugccg      360

ucgcauucaa cccggaagcu acaaacucgc agccagacau ggcucgaaag uagagaguac      420

accaaacacc ugaucagggu ugagaauugg auuuuucgga accccggauu cgcucuugcc      480

gcggcagcca ucgcuuggcu guugggcucc ucuacgagcc agaaggugau auaucucguc      540

augauccugc ugauugcccc ugcauacucc auccgcugua uuggcguguc aaaucgagac      600

uuuguagaag ggaugagugg agguacuugg guugauaucg ugcucgagca uggcgggugc      660

gucaccguga uggcucagga caagccaaca guggauauag aauuagucac uaccacaguu      720

agcaacaugg ccgaggugag aucuuacugu uaugaggcgu ccauuucaga cauggccagc      780

gauaguaggu gccccaccca aggagaagca uaccuggaca aacagucuga cacgcaguau      840

guauguaagc ggacucuugu ggaucguggc ugggguaaug ggugcggauu guucggcaaa      900

ggcucccugg ucacaugcgc uaaguuugcc uguagcaaaa agaugaccgg gaagucgauc      960

cagccugaga accuagaaua ccgcauuaug cucucugugc acggauccca acacucaggu     1020

augaucguua augacacugg ccaugagaca gaugaaaaca gagcuaaagu ggagaucacc     1080

ccaaauaguc ccagggccga ggcaacgcuu gggggauucg gcagccuggg ucuggacugc     1140

gaaccucgga ccggguugga uuuuuccgac cucuauuacc ugacaaugaa caauaagcac     1200

ugguuagucc auaaagagug guuccacgau auuccacugc cguggcacgc cggcgcugac     1260

acuggaacac cccauuggaa caauaaggaa gcgcucgugg aguuuaaaga ugcacacgcc     1320

aagcgacaga ccguaguugu gcuuggcucu caggaagggg cuguccauac ugcccuggca     1380

ggagccuugg aggcugagau ggacggugcc aaaggccgcc uaagcucagg gcaccugaag     1440

uguagacuca agauggauaa acugaggcuu aagggaguga guuauucccu gugcaccgca     1500

gcguucacau uuaccaaaau accugcugaa acuuugcaug gcacggucac aguggagguu     1560

caguacgccg guaccgacgg gccauguaag gugcccgcac aaauggccgu agacaugcag     1620

acucucaccc cugucggccg gcugaucaca gcuaacccag ugauuaccga aagcacugag     1680

aacucuaaaa ugauguuaga acuggauccc ccguucggag acucauacau cguuauaggc     1740

gugggggaga agaagauuac acaccacugg caucgcagug gaagcacgcu agguaaagcc     1800

uuuuccacca cucucaaggg cgcacagaga cuggcugccc uuggggauac agcgugggac     1860

uucggaucua ucggcggugu cuucaauucg aucgggaggg ccgugcacca aguauuuggc     1920

ggagcuuucc guaccuuguu uggcgggaug agcuggauua cacagggacu gaugggcgca     1980

cuccugcuuu ggaugggugu gaacgcccgg gaucgaucca ucgcucuggc cuucuuggca     2040

acugggggag uucucgucuu ucuggcgacc aaugugcaug cc                        2082


<210>  9822
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9822
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cuguuaccag gcgggguuca gccuacuaua uguaccuuga ccgcaacgau      120

gcaggcgagg ccauaaguuu ccccacaacu cuggggauga auaaauguua uauccagauu      180

auggacuugg gacacacgug cgaugcuacc augagcuacg aauguccuau guuagacgag      240

ggcguggaac cagaugacgu agauugcugg uguaacacaa cuuccaccug gguggucuau      300

gguacaugcc aucacaagaa aggggaggca cgacguucua gaagggccgu gacccugccg      360

ucgcauucaa cucggaagcu acaaacacgc agccagaccu ggcucgaaag uagagaguac      420

acgaaacacc ugaucagggu ugagaauugg auuuuucgga accccggauu cgcucuugcc      480

gcggcagcca ucgcuuggcu guugggcucc ucuacuagcc agaaggugau auaucucguc      540

augauccugc ugauugcccc ugcauacucc auccgcugua uuggcguguc aaaucgagac      600

uuuguagaag ggaugagugg agguaccugg guugaugugg uccucgagca uggcgggugc      660

gugacaguga uggcucagga caagccaacu gucgauaucg aauuaguuac cacaaccgug      720

agcaacaugg ccgagguaag aucuuacugu uaugaggcgu ccauaucaga cauggccagc      780

gauaguaggu gccccacgca aggagaagca uaccuggaca aacagucuga cacucaguau      840

guguguaagc ggacacuugu cgaucguggc ugggguaaug ggugcggauu guucggcaaa      900

ggcucccugg ugaccugcgc uaaguuugcc uguagcaaaa agaugacugg gaagucgauu      960

cagccugaga accuagaaua ccgcaucaug cucucuguuc acggauccca acacucaggu     1020

augauuguga augacacagg ccaugagacc gaugaaaaca gagcuaaagu cgagaucacg     1080

ccaaauaguc ccagggccga ggcaacccuu gggggauucg gcagccuggg ucuggacugc     1140

gaaccucgga caggguugga uuuuuccgac cucuauuacc ugacuaugaa caauaagcac     1200

ugguuagugc auaaagagug guuccacgau aucccacugc cguggcacgc cggcgcugac     1260

accggaacac cccauuggaa caauaaggaa gcgcucguag aguuuaaaga ugcacacgcc     1320

aagcgacaga cuguuguggu ccuuggcucu caggaagggg cugugcauac cgcccuggca     1380

ggagccuugg aggcugagau ggacggugcc aaaggccgcc uaagcucagg gcaccugaag     1440

uguagacuca agauggauaa acugaggcuu aagggaguca guuauucccu gugcacagca     1500

gcguucaccu uuacuaaaau uccugcugaa acguugcaug gcacagugac cguugaggug     1560

caguacgccg guacugacgg gccauguaag guacccgcac aaauggccgu cgacaugcag     1620

acccucacac cugugggccg gcugauaacc gcuaacccag uuaucacuga aagcacagag     1680

aacucuaaaa ugauguuaga acuggauccc ccguucggag acucauacau ugugaucggc     1740

gucggggaga agaagauaac gcaccacugg caucgcagug gaagcacccu agguaaagcc     1800

uuuuccacua cacucaaggg cgcacagaga cuggcugccc uuggggauac cgcgugggac     1860

uucggaucua uuggcggugu guucaauucg aucgggaggg ccguacacca aguguuuggc     1920

ggagcuuucc guacauuguu uggcgggaug agcuggauca cucagggacu gaugggcgca     1980

cuccugcuuu ggaugggugu uaacgcccgg gaucgaucca uugcucuggc cuucuuggca     2040

accgggggag uccucguguu ucuggcgacg aaugugcaug cc                        2082


<210>  9823
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9823
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cuauaaccag gcgggguuca gccuacuaua uguaccuuga ccgcagugau      120

gcaggcaaag ccaucagcuu cgcuacaacu cuggggguua acaaguguca cgugcagauu      180

auggacuugg gacauaugug cgaugcaacg auguccuaug agugucccau guuagacgaa      240

ggcguagagc cugaugacgu ggauugcugg uguaauacca caucuacuug ggucguguac      300

gguaccugcc accauaaaaa gggggaagcc cgacguucga gaagggcugu uacacugcca      360

ucacacagca cccggaaacu acaaacucgc agucagacau ggcucgaguc cagagaauau      420

accaagcauc ugaucaaggu ggagaacugg auuuuuagga auccgggauu cgcccuuguc      480

gcgguggcaa ucgccuggcu guugggcucu agcacguccc agaaaguaau auaccucguu      540

augauccugc ugauugcucc cgccuauuca auccggugua uuggcgugag uaaccgcgac      600

uuugucgagg ggaugagcgg agguacuugg guggaugugg uccucgaaca cggcgggugc      660

guuaccguga uggcacagga caagccuaca guagauaucg aguuagugac uaccacaguc      720

ucuaauaugg cugaagugcg auccuacugu uacgaggccu caauaagcga cauggcgagu      780

gauucuagau gcccaaccca aggagaggcc uaucuggaca aacaguccga cacgcaguac      840

guuuguaaga ggacucuugu ggaucggggc ugggguaacg ggugcggauu guucggcaaa      900

ggcagccugg ucacaugcgc aaaguuuacc uguucgaaga aaaugacugg gaagucuauu      960

cagcccgaaa aucuagagua ucguaucaug cucuccgugc acggaucaca acauaguggu     1020

augauuggcu acgaaacaga cgaggaucgc gcuaaaguag aaguuacccc uaacagccca     1080

agagccgagg cuacgcuugg gggauucggc ucccuggguc uggacugcga gcccaggacc     1140

ggguuggauu uuucugaccu cuauuaccug acaaugaaua acaagcacug guuagugcau     1200

aaagaauggu uccacgauau cccucugcca uggcacgccg gcgcagacac uggaaccccg     1260

cauuggaaua acaaggaggc ccucgucgaa uuuaaagaug cucacgcgaa gcggcagaca     1320

guggucgugc uuggcagcca ggagggggca guucauacug cccuggcugg agccuuggaa     1380

gcagagaugg acggugccaa gggccgacua uucucagggc accugaaaug ucgccucaag     1440

auggauaaac ugagacuuaa gggagugagu uauucccugu gcaccgcugc cuuuacauuc     1500

accaaaguac ccgcagagac uuugcauggc acggucacag uggaaguuca guacgcgggu     1560

accgacgggc cuuguaagau cccagugcaa auggcugucg acaugcagac ucucaccccc     1620

gugggcaggc ugauuacagc caauccugua auaaccgaga gcacugaaaa cucuaagaug     1680

auguuagagc uggauccacc cuuuggagac ucauacaucg ugauuggcgu uggggauaaa     1740

aagaucacac accacuggca ucggagugga agcacgcuag guaaagcauu cuccaccacu     1800

cucaagggcg cccagcgccu ggcugcccuu ggggacacag caugggauuu cggaucuaua     1860

ggcggugucu uuaauucgau ugggagagcu gugcaccaag uguucggcgg agccuuuagg     1920

accuuguucg gcgggaugag cuggaucaca cagggacuga ugggcgcgcu ccugcuuugg     1980

auggguguca acgcccguga ccgguccauc gcucuggcau uuuuggccac ugggggaguu     2040

cucguguucc uggcuaccaa cgugcaugcc                                      2070


<210>  9824
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9824
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cuguuaccag gcgggguuca gccuacuaua uguaccuuga ccgcaacgau      120

gcaggcgagg ccauaaguuu ccccacaacu cuggggauga auaaauguua uauccagauu      180

auggacuugg gacacaugug cgaugcuacg augagcuacg aauguccuau guuagacgag      240

ggcguggaac cagaugacgu agauugcugg uguaacacca cauccacuug gguggucuau      300

gguaccugcc aucacaagaa aggggaggca cgacguucua gaagggccgu gacacugccg      360

ucgcauucaa cccggaagcu acaaacucgc agccagacau ggcucgaaag uagagaguac      420

accaaacacc ugaucagggu ugagaauugg auuuuucgga accccggauu cgcucuugcc      480

gcggcagcca ucgcuuggcu guugggcucc ucuacgagcc agaaggugau auaucucguc      540

augauccugc ugauugcccc ugcauacucc auccgcugua uuggcguguc aaaucgagac      600

uuuguagaag ggaugagugg agguacuugg guugaugugg uccucgagca uggcgggugc      660

gugaccguga uggcucagga caagccaaca gucgauaucg aauuaguuac uaccacagug      720

agcaacaugg ccgagguaag aucuuacugu uaugaggcgu ccauaucaga cauggccagc      780

gauaguaggu gccccaccca aggagaagca uaccuggaca aacagucuga cacgcaguau      840

guguguaagc ggacucuugu cgaucguggc ugggguaaug ggugcggauu guucggcaaa      900

ggcucccugg ugacaugcgc uaaguuugcc uguagcaaaa agaugaccgg gaagucgauu      960

cagccugaga accuagaaua ccgcaucaug cucucuguuc acggauccca acacucaggu     1020

augauuguga augacacugg ccaugagaca gaugaaaaca gagcuaaagu cgagaucacc     1080

ccaaauaguc ccagggccga ggcaacgcuu gggggauucg gcagccuggg ucuggacugc     1140

gaaccucgga ccggguugga uuuuuccgac cucuauuacc ugacaaugaa caauaagcac     1200

ugguuagugc auaaagagug guuccacgau aucccacugc cguggcacgc cggcgcugac     1260

acuggaacac cccauuggaa caauaaggaa gcgcucguag aguuuaaaga ugcacacgcc     1320

aagcgacaga ccguuguggu ccuuggcucu caggaagggg cugugcauac ugcccuggca     1380

ggagccuugg aggcugagau ggacggugcc aaaggccgcc uaagcucagg gcaccugaag     1440

uguagacuca agauggauaa acugaggcuu aagggaguca guuauucccu gugcaccgca     1500

gcguucacau uuaccaaaau uccugcugaa acuuugcaug gcacggugac aguugaggug     1560

caguacgccg guaccgacgg gccauguaag guacccgcac aaauggccgu cgacaugcag     1620

acucucaccc cugugggccg gcugauaaca gcuaacccag uuaucaccga aagcacugag     1680

aacucuaaaa ugauguuaga acuggauccc ccguucggag acucauacau ugugaucggc     1740

gucggggaga agaagauaac acaccacugg caucgcagug gaagcacgcu agguaaagcc     1800

uuuuccacca cucucaaggg cgcacagaga cuggcugccc uuggggauac agcgugggac     1860

uucggaucua uuggcggugu guucaauucg aucgggaggg ccguacacca aguguuuggc     1920

ggagcuuucc guaccuuguu uggcgggaug agcuggauca cacagggacu gaugggcgca     1980

cuccugcuuu ggaugggugu uaacgcccgg gaucgaucca uugcucuggc cuucuuggca     2040

acugggggag uccucguguu ucuggcgacc aaugugcaug cc                        2082


<210>  9825
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9825
augaccagcg ugggcauugu cgggcugcuc cuuacaacug ccauggcugc aguuacgcug       60

cccucccacu cuaccagaaa guugcagaca aggucacaaa cuugguuaga gagucgggaa      120

uacaccaaac aucugauucg cguggagaac uggauauucc gaaauccugg auuugcccua      180

gcggcugccg caaucgccug gcuccugggu agcuccacau cucagaaggu aauuuaucuu      240

gugaugaucc uguugauugc uccagcauac ucgauccguu gcauaggcgu cucaaacaga      300

gacuucgugg aagggaugag cggaggcacc uggguugaua ucgugcucga gcacgguggg      360

ugugucacug ugauggccca ggacaaaccg acaguagaua uugaacuggu uaccacgacu      420

gugaguaaua uggcugaggu cagguccuau ugcuacgagg ccucuaucag cgacauggcg      480

uccgauucac ggugucccac ccagggagaa gcauaucugg acaagcaaag ugauacacag      540

uacgugugca aacgcacucu cguggacaga ggcuggggca acgggugugg auuauuuggu      600

aagggcagcc uggucacaug cgccaaguuc gcuuguucua aaaagaugac agggaaaucc      660

auucagccug agaaucuuga auauaggauc auguugucag uucauggaag ccagcacagu      720

ggcaugauag ugaacgauac cggucaugag acggacgaga aucgggccaa gguagaaauu      780

acuccaaacu cuccccgcgc agaggcuaca cuagggggau uuggcucccu gggccucgau      840

ugcgaaccuc gaaccgggcu ggacuucagc gaucuuuacu accugacuau gaauaacaaa      900

cacugguugg ugcacaagga gugguuucau gacaucccac uccccuggca cgccggagcg      960

gacacaggua ccccucauug gaauaacaag gaagcccugg ucgaguucaa agaugcacac     1020

gcuaagagac aaacgguggu uguguuaggc ucgcaggagg gggccgucca caccgcucug     1080

gccggagcac ucgaagccga gauggacggc gcuaaaggua ggcuuucuuc cgggcaucug     1140

aagugucggu ugaaaaugga uaagcuacgu cugaaaggcg ugucauauag ucucugcaca     1200

gcggcauuua cuuucaccaa gauuccagcc gaaacacugc acggaacugu aaccguugag     1260

gugcaguacg cuggcacaga cgggccgugu aagguccccg cccagauggc aguggauaug     1320

caaacccuua cuccugucgg acgccugauc acggccaauc cagugaucac agaaagcacc     1380

gagaacucca aaaugauguu ggagcucgau cccccuuuug gugacucuua uauuguuaua     1440

ggcguggggg aaaagaaaau cacucaucac uggcauagaa gcggaucaac cauuggcaag     1500

gcuuucgagg ccacaguaag gggugcaaaa cggauggcgg uccuggggga caccgcuugg     1560

gauuuuggca gugugggagg cgccuuaaau ucccugggga agggaaucca ccagauauuc     1620

ggugcagccu ucaagagccu auuuggcggg augucuuggu ucucacagau ucucaucgga     1680

acucugcuua ugugguuggg ccugaacacc aaaaacggua guaucagccu caugugccug     1740

gcucuugggg gcguucugau cuuuuugucc acggccgugu cugca                     1785


<210>  9826
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9826
augaccagcg ugggcaucgu cgggcugcuc cuuacaacug ccauggcugc aguuacgcug       60

cccucccacu cuaccagaaa guugcagaca aggucacaaa cuugguuaga gagucgggaa      120

uacaccaaac aucugauucg cguggagaac uggauauucc gaaauccugg auuugcccua      180

gcggcugccg caaucgccug gcuccugggu agcuccacau cucagaaggu aauuuaucuu      240

gugaugaucc uguugauugc uccagcauac ucgauccguu gcauaggcgu cucaaacaga      300

gacuucgugg aagggaugag cggaggcacc uggguugaug ugguccucga gcacgguggg      360

ugugugacug uaauggccca ggacaaaccg acaguugaua ucgaacuggu gaccacgacu      420

gucaguaaua uggcugaggu gagguccuau ugcuacgagg ccucuauuag cgacauggcg      480

uccgauucac ggugucccac ccagggagaa gcauaucugg acaagcaaag ugauacacag      540

uacgugugca aacgcacucu cgucgacaga ggcuggggca acgggugugg auuauuuggu      600

aagggcagcc ugguuaccug cgccaaguuc gcuuguucua aaaagaugac agggaaaucc      660

auccagccug agaaucuuga auauaggauu auguugucag ugcauggaag ccagcacagu      720

ggcaugaucg uaaacgauac cggucaugag acggacgaga aucgggccaa gguggaaaua      780

acuccaaacu cuccccgcgc agaggcuaca cugggaggau uuggcucccu aggccucgau      840

ugcgaaccuc gaaccgggcu ggacuucagc gaucuuuacu accugacuau gaauaacaaa      900

cacugguugg uccacaagga gugguuucau gacauuccac uccccuggca cgccggagcg      960

gacacaggua ccccucauug gaauaacaag gaagcccugg uggaguucaa agaugcacac     1020

gcuaagagac aaacgguugu ggucuuaggc ucgcaggagg gggccgugca caccgcucug     1080

gccggagcac ucgaagccga gauggacggc gcuaaaggua ggcuuucuuc cgggcaucug     1140

aagugucggu ugaaaaugga uaagcuacgu cugaaaggcg uaucauauag ucucugcaca     1200

gcggcauuua cuuucaccaa gaucccagcc gaaacacugc acggaacugu uaccguggag     1260

guccaguacg cuggcacaga cgggccgugu aaggugcccg cccagauggc agucgauaug     1320

caaacccuua cuccuguggg acgccugauu acggccaauc caguuaucac agaaagcacc     1380

gagaacucca aaaugauguu ggagcucgau cccccuuuug gugacucuua uaucgugauu     1440

ggcguagggg aaaagaaaau aacucaucac uggcauagaa gcggaucaac caucggcaag     1500

gcuuucgagg ccacagucag gggugcaaaa cggauggcgg ugcuggggga caccgcuugg     1560

gauuuuggca guguuggagg cgccuuaaau ucccugggga aggggauuca ccagaucuuc     1620

ggugcagccu ucaagagccu auuuggcggg augucuuggu ucucacagau acucauugga     1680

acucugcuua ugugguuggg ccugaacgcu aaaaacggua guaucagccu caugugccug     1740

gcccuugggg gcgugcugau cuuuuugucc acagcagucu cugcu                     1785


<210>  9827
<211>  1773
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9827
augaccagca ucggcauuau agggcugcuc cuuacaacug ccauggcugc agugacgcug       60

cccucccacu cuaccagaaa guugcagaca aggucacaaa cuugguuaga gagucgggaa      120

uacaccaaac aucugaucaa ggucgagaac uggauuuucc gcaauccugg auuugcccua      180

guugcggugg cuaucgccug gcuccugggu agcuccacau cucagaaagu aauuuaucuu      240

gugaugaucc uguugauagc accagccuac ucgauccgau gcauuggcgu cucaaaccgu      300

gacuucgugg aagggaugag cggaggcacc uggguugaug ugguccucga gcacgguggg      360

ugugugacug uaauggcuca ggacaagccg acaguugaua ucgaacuggu gaccacgacu      420

gucaguaaua uggcagaggu gagauccuau ugcuacgagg ccucuauuag cgacauggcu      480

uccgauucaa ggugucccac ccagggagaa gccuaucugg acaaacaaag ugauacacag      540

uacgugugca agcggacucu cgucgaccgc ggcuggggca acgggugugg auuauuuggu      600

aagggcagcc ugguuaccug cgcgaaauuc acauguucua agaaaaugac cgggaagucc      660

auccagccug agaaucuuga auauagaaua auguugucag ugcauggaag ccagcacagu      720

ggcaugauug guuacgagac ggaugaggac agggcaaaag uagaagugac uccaaacucu      780

ccccgggccg aggcuacacu ugggggauuu ggcucccuag gccucgauug cgaaccucgc      840

accgggcugg acuucagcga ucuuuacuau cugacuauga auaacaagca uugguugguc      900

cacaaggagu gguuucacga caucccacuc cccuggcaug ccggagcaga cacagguacc      960

ccucacugga auaacaaaga agcucuggug gaguucaagg augcccaugc gaaacgacaa     1020

acgguugugg ucuuaggcuc gcaggagggg gccgugcaca ccgcacuggc uggagcccuc     1080

gaagcugaga uggacggcgc caaggguaga cuguuuucug ggcaccugaa auguagguug     1140

aagauggaua aacuacggcu gaagggcgua uccuacucac ucugcacagc agccuucacu     1200

uuuaccaagg uuccagcuga aacacugcau ggaacuguga ccgucgaggu gcaguaugcg     1260

ggcacagacg ggccguguaa aauucccguc cagauggcag uggauaugca aacccuuacu     1320

ccuguuggac gucugaucac ggccaaucca gugaucacag aaaguaccga gaacagcaag     1380

augauguugg agcucgaucc cccuuucggu gacuccuaca uuguaauagg cgucggggac     1440

aaaaagauca cucaccauug gcaccgcucu ggaagcacca uuggcaaggc uuuugaagcc     1500

acagugagag gugcaaaaag gauggccguu cugggggaua ccgcuuggga cuucggcuca     1560

gugggaggcg ucuucaauag uuuagggaag ggaauccauc agauauuugg ugccgcauuc     1620

aaaucccugu uuggcgggau gagcugguuc ucucagauuc uaaucggaac ucuccuggug     1680

uggcuuggcu ugaacacaaa gaaugguuca aucagucuga cgugccucgc gcuugggggc     1740

guaaugauuu uucugagcac cgcugugucc gcc                                  1773


<210>  9828
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9828
augaccagcg ugggcaucgu cgggcugcuc cuuacaacug ccauggcugc aguuacgcug       60

cccucccacu cuaccagaaa guugcagaca aggucacaaa cuugguuaga gagucgggaa      120

uacaccaaac aucugauucg cguggagaac uggauauucc gaaauccugg auuugcccua      180

gcggcugccg caaucgccug gcuccugggu agcuccacau cucagaaggu aauuuaucuu      240

gugaugaucc uguugauugc uccagcauac ucgauccguu gcauaggcgu cucaaacaga      300

gacuucgugg aagggaugag cggaggcacc uggguugaug ugguccucga gcacgguggg      360

ugugugacug uaauggccca ggacaaaccg acaguugaua ucgaacuggu gaccacgacu      420

gucaguaaua uggcugaggu gagguccuau ugcuacgagg ccucuauuag cgacauggcg      480

uccgauucac ggugucccac ccagggagaa gcauaucugg acaagcaaag ugauacacag      540

uacgugugca aacgcacucu cgucgacaga ggcuggggca acgggugugg auuauuuggu      600

aagggcagcc ugguuaccug cgccaaguuc gcuuguucua aaaagaugac agggaaaucc      660

auccagccug agaaucuuga auauaggauu auguugucag ugcauggaag ccagcacagu      720

ggcaugaucg uaaacgauac cggucaugag acggacgaga aucgggccaa gguggaaaua      780

acuccaaacu cuccccgcgc agaggcuaca cugggaggau uuggcucccu aggccucgau      840

ugcgaaccuc gaaccgggcu ggacuucagc gaucuuuacu accugacuau gaauaacaaa      900

cacugguugg uccacaagga gugguuucau gacauuccac uccccuggca cgccggagcg      960

gacacaggua ccccucauug gaauaacaag gaagcccugg uggaguucaa agaugcacac     1020

gcuaagagac aaacgguugu ggucuuaggc ucgcaggagg gggccgugca caccgcucug     1080

gccggagcac ucgaagccga gauggacggc gcuaaaggua ggcuuucuuc cgggcaucug     1140

aagugucggu ugaaaaugga uaagcuacgu cugaaaggcg uaucauauag ucucugcaca     1200

gcggcauuua cuuucaccaa gaucccagcc gaaacacugc acggaacugu uaccguggag     1260

guccaguacg cuggcacaga cgggccgugu aaggugcccg cccagauggc agucgauaug     1320

caaacccuua cuccuguggg acgccugauu acggccaauc caguuaucac agaaagcacc     1380

gagaacucca aaaugauguu ggagcucgau cccccuuuug gugacucuua uaucgugauu     1440

ggcguagggg aaaagaaaau aacucaucac uggcauagaa gcggaucaac caucggcaag     1500

gcuuucgagg ccacagucag gggugcaaaa cggauggcgg ugcuggggga caccgcuugg     1560

gauuuuggca guguuggagg cgccuuaaau ucccugggga aggggauuca ccagaucuuc     1620

ggugcagccu ucaagagccu auuuggcggg augucuuggu ucucacagau acucauugga     1680

acucugcuua ugugguuggg ccugaacacg aaaaacggua guaucagccu caugugccug     1740

gcucuugggg gcgugcugau cuuuuugucc acagccgucu cugca                     1785


<210>  9829
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9829
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgcggu gacccuuccu ucacacagua caagaaagcu gcaaacuagg      120

agccagacgu gguuggaauc ccgggaguac accaaacauu uaauccgcgu agaaaacugg      180

auuuuccgaa auccaggauu ugcucuggcc gcagccgcua ucgcauggcu acucgguucu      240

ucgacaucac agaaggugau uuaucugguc augauccuuc ugauagcccc ggcuuacagc      300

auccguugca uuggcgugag uaacagagac uucguugagg ggauguccgg aggcacuugg      360

guggauaucg ucuuggaaca cggugggugu gugaccguaa uggcccagga caaacccaca      420

guugauauug agcucgugac cacuacaguc ucuaauaugg cggaggugag gagcuauugc      480

uacgaagcau ccaucucaga cauggccagu gauagccggu guccuaccca aggagaggcu      540

uaucuggaca agcagucuga uacgcaguac gugugcaaac gcacucuggu cgacagaggc      600

uggggcaacg gguguggacu cuuugguaag ggcuccuuag uuaccugcgc caaguucgca      660

uguagcaaaa agaugacagg gaaaucaaua cagccagaaa aucuggagua uaggauuaug      720

cuuagugugc auggaucuca acacuccggc augaucguaa acgauacugg ucaugagacc      780

gacgaaaauc gggcuaaggu ggagauuaca cccaacagcc cucgcgccga agcgaccuug      840

ggaggauuug gcucgcuggg ccuagauugc gagccacgaa cggggcucga cuucucugau      900

cuguacuacc uuacuaugaa uaacaaacac uggcuggucc acaaggaaug guuucaugac      960

auccccuugc cuuggcacgc cggagcagac acagguaccc cacauuggaa uaacaaggag     1020

gcucucgugg aguucaaaga ugcccacgcu aagagacaga cuguuguggu ccugggcucc     1080

caggaagggg ccgugcacac agcauuagcc ggagcucugg aggcggaaau ggacggcgca     1140

aaagguaggc ucucaagugg gcaucuuaag ugucggcuga aaauggauaa guugcgucua     1200

aaaggcguaa gcuauucccu gugcaccgcc gcuuuuacgu ucaccaagau cccggccgag     1260

acacuccacg gaacuguuac cguggaaguc caguacgcag gcacagacgg gcccuguaag     1320

gugccugccc aaauggcugu cgauaugcag acucugaccc cagugggacg ccuuauuaca     1380

gccaaucccg uuauaaccga gucuacugag aacagcaaaa ugaugcugga auuggacccu     1440

ccauuuggug auucauauau cgugauuggc guaggggaga agaaaaucac gcaucacugg     1500

cauagaagug gauccacaau aggcaaggca uucgaagcga ccgucagggg ugcuaaacgg     1560

auggccgugc ucggggacac ugcaugggau uucggcagcg uuggaggcgc cuuaaauucu     1620

cuggggaagg ggauucacca gaucuuuggu gcugccuuca agucacuguu uggcgggaug     1680

aguugguuca gccaaauccu aauuggaacc cuccugaugu ggcuuggcuu gaacaccaaa     1740

aacgguucca ucucucugau gugccucgca cuugggggcg ugcugauuuu ucugucgaca     1800

gcugucagcg cc                                                         1812


<210>  9830
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9830
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgcggu gacccuuccu ucacacagua caagaaagcu gcaaacuagg      120

agccagacgu gguuggaauc ccgggaguac accaaacauu uaauccgcgu agaaaacugg      180

auuuuccgaa auccaggauu ugcucuggcc gcagccgcua ucgcauggcu acucgguucu      240

ucgacaucac agaaggugau uuaucugguc augauccuuc ugauagcccc ggcuuacagc      300

auccguugca uuggcgugag uaacagagac uucguugagg ggauguccgg aggcacuugg      360

guggaugucg uguuggaaca cggugggugu guaaccguua uggcccagga caaacccaca      420

guggauaucg agcucgucac cacuacagug ucuaauaugg cggaggugag gagcuauugc      480

uacgaagcau ccauuucaga cauggccagu gauagccggu guccuaccca aggagaggcu      540

uaucuggaca agcagucuga uacgcaguac gucugcaaac gcacucuggu ugacagaggc      600

uggggcaacg gguguggacu cuuugguaag ggcuccuuag ugaccugcgc caaguucgca      660

uguagcaaaa agaugacagg gaaaucaauc cagccagaaa aucuggagua uaggauaaug      720

cuuaguguac auggaucuca acacuccggc augauuguga acgauacugg ucaugagacc      780

gacgaaaauc gggcuaaggu cgagaucaca cccaacagcc cucgcgccga agcgaccuug      840

ggaggguuug gcucgcuggg ccuagauugc gagccacgaa cggggcucga cuucucugau      900

cuguacuacc uuacuaugaa uaacaaacac uggcuggugc acaaggaaug guuucaugac      960

auucccuugc cuuggcacgc cggagcagac acagguaccc cacauuggaa uaacaaggag     1020

gcucucguug aguucaaaga ugcccacgcu aagagacaga cuguggucgu gcugggcucc     1080

caggaagggg ccguacacac agcauuagcc ggagcucugg aggcggaaau ggacggcgca     1140

aaagguaggc ucucaagugg gcaucuuaag ugucggcuga aaauggauaa guugcgucua     1200

aaaggcguua gcuauucccu gugcaccgcc gcuuuuacgu ucaccaagau cccggccgag     1260

acacuccacg gaacugugac cgucgaagug caguacgcag gcacagacgg gcccuguaag     1320

gucccugccc aaauggcugu ggauaugcag acucugaccc caguuggacg ccuuaucaca     1380

gccaaucccg ugauuaccga gucuacugag aacagcaaaa ugaugcugga auuggacccu     1440

ccauuuggug auucauauau aguaaucggc gucggggaga agaaaauuac gcaucacugg     1500

cauagaagug gauccacaau cggcaaggca uucgaagcga ccgugagggg ugcuaaacgg     1560

auggccguuc ucggggacac ugcaugggau uucggcagcg ugggaggcgc cuuaaauucu     1620

cuggggaagg gaauacacca gauuuuuggu gcugccuuca agucacuguu uggcgggaug     1680

aguugguuca gccaaauccu aaucggaacc cuccugaugu ggcuuggcuu gaacgcuaaa     1740

aacgguucca uuucucugau gugccucgca cuugggggcg uccugaucuu ucugucgaca     1800

gccgugagcg cg                                                         1812


<210>  9831
<211>  1800
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9831
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgcggu gacccuuccu ucacacagua caagaaagcu gcaaacuagg      120

agccagacgu gguuggaauc ccgggaguac accaaacauu uaaucaaggu agaaaacugg      180

auuuuccgca auccaggauu ugcucuggug gccgucgcaa ucgccuggcu acucgguucu      240

ucgacaucac agaaagugau uuaucugguu augauccuuc ugauagcucc ggcauacagc      300

auccgaugca uuggcgugag uaaccgugac uucgucgagg ggauguccgg aggcacuugg      360

guggauguag uuuuggaaca cggugggugu gugaccguca uggcccagga caagcccaca      420

guggauaucg agcucgugac cacuacaguc ucuaauaugg cugagguuag aagcuauugc      480

uacgaagccu ccauuucaga cauggcgagu gauucuaggu guccuaccca aggagaggca      540

uaucuggaca aacagagcga uacgcaguac gugugcaagc ggacucuggu agaccgcggc      600

uggggcaacg gguguggacu cuuugguaag ggcuccuuag ugaccugcgc caaauucaca      660

uguucaaaga aaaugacugg aaagagcauc cagccagaaa aucuggagua uagaauaaug      720

cuuagugucc augggucuca acacuccggc augauugguu acgagaccga ugaagacagg      780

gcuaaagugg agguuacacc caacagcccu cgggccgaag caaccuuggg aggguuuggc      840

ucgcugggcc uagauugcga gccacgcacg gggcucgacu ucucugaucu guacuaucuu      900

acuaugaaua acaagcauug gcuggugcac aaggaauggu uucacgacau ccccuugccu      960

uggcaugcug gagccgacac agguacccca cacuggaaua acaaagaggc gcucgucgag     1020

uucaaggaug cccaugcaaa acgacagacu gugguaguuc ugggcuccca ggaaggggcu     1080

gugcacacag ccuuagcugg agcccuggag gcagaaaugg acggcgccaa ggguagacuc     1140

uuuucagggc accuuaaaug uaggcugaag auggauaaau ugcggcuaaa gggcgucagu     1200

uacagccugu gcaccgcugc guucacguuu accaaggugc cggcagagac acuccaugga     1260

acugucaccg uggaaguuca guaugccggc acagacgggc ccuguaaaau uccugugcaa     1320

auggcuguag auaugcagac ucugacccca gucggacguc uuaucacagc caaucccgug     1380

aucaccgagu ccacugagaa cucuaagaug augcuggaau uggacccucc auucggugau     1440

agcuacauug uuauaggcgu gggggacaaa aagaucacgc accauuggca ccgcucagga     1500

aguacaauug gcaaagcauu cgaggccacc gucagaggug cuaagaggau ggccgugcuc     1560

ggggauacug caugggacuu uggcuccgua ggaggcgugu ucaauagccu ggggaaggga     1620

auccaucaga uauuuggugc ggcuuucaaa ucuuuauuug gcgggauguc augguucagu     1680

caaauucuga ucggaacccu acucguuugg cugggccuua acacaaagaa ugguagcauc     1740

uccuugaccu gccuggcccu cgggggcguc augauuuuuc ugucuacugc agugucggcc     1800


<210>  9832
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9832
auggccauca gcggcgugcc cguccugggg uucuuuauua uagcuguucu cauguccgca       60

caggagucuu gggccgcggu gacccuuccu ucacacagua caagaaagcu gcaaacuagg      120

agccagacgu gguuggaauc ccgggaguac accaaacauu uaauccgcgu agaaaacugg      180

auuuuccgaa auccaggauu ugcucuggcc gcagccgcua ucgcauggcu acucgguucu      240

ucgacaucac agaaggugau uuaucugguc augauccuuc ugauagcccc ggcuuacagc      300

auccguugca uuggcgugag uaacagagac uucguugagg ggauguccgg aggcacuugg      360

guggaugucg uguuggaaca cggugggugu guaaccguua uggcccagga caaacccaca      420

guggauaucg agcucgucac cacuacagug ucuaauaugg cggaggugag gagcuauugc      480

uacgaagcau ccauuucaga cauggccagu gauagccggu guccuaccca aggagaggcu      540

uaucuggaca agcagucuga uacgcaguac gucugcaaac gcacucuggu ugacagaggc      600

uggggcaacg gguguggacu cuuugguaag ggcuccuuag ugaccugcgc caaguucgca      660

uguagcaaaa agaugacagg gaaaucaauc cagccagaaa aucuggagua uaggauaaug      720

cuuaguguac auggaucuca acacuccggc augauuguga acgauacugg ucaugagacc      780

gacgaaaauc gggcuaaggu cgagaucaca cccaacagcc cucgcgccga agcgaccuug      840

ggaggguuug gcucgcuggg ccuagauugc gagccacgaa cggggcucga cuucucugau      900

cuguacuacc uuacuaugaa uaacaaacac uggcuggugc acaaggaaug guuucaugac      960

auucccuugc cuuggcacgc cggagcagac acagguaccc cacauuggaa uaacaaggag     1020

gcucucguug aguucaaaga ugcccacgcu aagagacaga cuguggucgu gcugggcucc     1080

caggaagggg ccguacacac agcauuagcc ggagcucugg aggcggaaau ggacggcgca     1140

aaagguaggc ucucaagugg gcaucuuaag ugucggcuga aaauggauaa guugcgucua     1200

aaaggcguua gcuauucccu gugcaccgcc gcuuuuacgu ucaccaagau cccggccgag     1260

acacuccacg gaacugugac cgucgaagug caguacgcag gcacagacgg gcccuguaag     1320

gucccugccc aaauggcugu ggauaugcag acucugaccc caguuggacg ccuuaucaca     1380

gccaaucccg ugauuaccga gucuacugag aacagcaaaa ugaugcugga auuggacccu     1440

ccauuuggug auucauauau aguaaucggc gucggggaga agaaaauuac gcaucacugg     1500

cauagaagug gauccacaau cggcaaggca uucgaagcga ccgugagggg ugcuaaacgg     1560

auggccguuc ucggggacac ugcaugggau uucggcagcg ugggaggcgc cuuaaauucu     1620

cuggggaagg gaauacacca gauuuuuggu gcugccuuca agucacuguu uggcgggaug     1680

aguugguuca gccaaauccu aaucggaacc cuccugaugu ggcuuggcuu gaacaccaaa     1740

aacgguucca uuucucugau gugccucgca cuugggggcg uccugaucuu ucugucgaca     1800

gcugugagcg cc                                                         1812


<210>  9833
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9833
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcgccguu       60

acucuucccu cccauucuac gaggaagcug cagacccggu cacaaacaug guuggagagu      120

cgcgaauaca cuaaacacuu aauucgagug gagaacugga uauuucguaa uccuggcuuc      180

gcgcuggcug ccgcagccau cgcuuggcua cucgggagcu ccaccucuca gaagguaauu      240

uaucugguga ugauccuucu gauugcacca gccuacucga ucagaugcau aggagucuca      300

aacagggauu uuguggaagg uaugagcggc gggacauggg uugacaucgu guuggagcau      360

ggaggcugug ucaccgugau ggcucaggau aaaccgacug uagacauuga acucguuaca      420

accacgguga guaauauggc cgagguccgg uccuauugcu acgaggcguc uaucagcgau      480

auggcauccg acucacgcug ucccacucag ggugaagccu aucuggauaa gcaaagugac      540

acccaguacg ugugcaaaag aacacuggug gauagggggu ggggaaacgg cuguggccuc      600

uucgggaagg ggagcuuagu cacuugcgcu aaguuugccu guucuaaaaa gaugaccggu      660

aaauccauuc agccugagaa ucuggaauau cggaucaugc uuucaguuca cggcagccag      720

cauaguggga ugauagugaa cgacacagga cacgagaccg augagaaucg cgcaaaggua      780

gaaauuacgc caaacucucc ccgagcugag gccacuuugg gcgguuucgg gucccuggga      840

cuagacugcg aaccuagaac aggccucgau uuuagcgacc uguacuaccu uaccaugaau      900

aacaaacacu ggcuggugca uaaggagugg uuccacgaca ucccauugcc cuggcaugcg      960

ggcgccgaua cugggacacc ucacuggaau aacaaggaag cacucgucga guuuaaagac     1020

gcucacgcca agaggcaaac cgugguugug cugggaucgc aggagggugc uguccauacg     1080

gccuuagcag gcgcccugga agcugagaug gauggggcga aaggacggcu cucuuccggc     1140

caccuuaagu gucgucugaa aauggacaag uugcgccuaa aagguguguc auauagucug     1200

ugcaccgcag ccuucacauu uacuaagauu ccagcugaaa cccuccaugg gacaguaacu     1260

guugaggugc aguacgccgg caccgaugga ccguguaagg uccccgcaca gauggccgug     1320

gacaugcaaa cacugacccc ugucggcaga cuuaucacug cuaauccagu gaucacggaa     1380

agcacagaga acuccaaaau gaugcuggag uuggaucccc cuuucgggga cucuuauauu     1440

guuauaggag ugggugaaaa gaaaaucacc caccauuggc acaggagcgg cucaacuauu     1500

gggaaggccu uugaggcaac cguacgggga gcgaaacgaa uggcuguccu cggcgauaca     1560

gccugggacu ucgguagugu gggaggcgca uuaaauuccc ugggaaaggg cauccaccag     1620

auauucgggg ccgcuuuuaa gagccuguuc ggagguaugu cuugguuuuc acagauucua     1680

aucggcaccc uccugaugug gcuuggguug aacacuaaaa acggaaguau cagccugaug     1740

ugccucgccc ugggcggugu ucuuauuuuc cuguccacag cagugucugc u              1791


<210>  9834
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9834
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcgccguu       60

acucuucccu cccauucuac gaggaagcug cagacccggu cacaaacaug guuggagagu      120

cgcgaauaca cuaaacacuu aauucgagug gagaacugga uauuucguaa uccuggcuuc      180

gcgcuggcug ccgcagccau cgcuuggcua cucgggagcu ccaccucuca gaagguaauu      240

uaucugguga ugauccuucu gauugcacca gccuacucga ucagaugcau aggagucuca      300

aacagggauu uuguggaagg uaugagcggc gggacauggg uugacguggu cuuggagcau      360

ggaggcugug ugaccguaau ggcucaggau aaaccgacug uugacaucga acucgugaca      420

accacgguca guaauauggc cgaggugcgg uccuauugcu acgaggcguc uauuagcgau      480

auggcauccg acucacgcug ucccacucag ggugaagccu aucuggauaa gcaaagugac      540

acccaguacg ugugcaaaag aacacugguc gauagggggu ggggaaacgg cuguggccuc      600

uucgggaagg gaagccuggu uacuugcgcu aaguuugccu guucuaaaaa gaugaccggu      660

aaauccaucc agccugagaa ucuggaauau cggauuaugc uuucagugca cggcagccag      720

cauaguggga ugaucguaaa cgacacagga cacgagaccg augagaaucg cgcaaaggug      780

gaaauaacgc caaacucucc ccgagcugag gccacuuugg gcgguuucgg gucccuggga      840

cuagacugcg aaccuagaac aggccucgau uuuagcgacc uguacuaccu uaccaugaau      900

aacaaacacu ggcuggucca uaaggagugg uuccacgaca uuccauugcc cuggcaugcg      960

ggcgccgaua cugggacacc ucacuggaau aacaaggaag cacucgugga guuuaaagac     1020

gcucacgcca agaggcaaac cguugugguc cugggaucgc aggagggugc ugugcauacg     1080

gccuuagcag gcgcccugga agcugagaug gauggggcga aaggacggcu cucuuccggc     1140

caccuuaagu gucgucugaa aauggacaag uugcgccuaa aagguguauc auauagucug     1200

ugcaccgcag ccuucacauu uacuaagauc ccagcugaaa cccuccaugg gacaguuacu     1260

guggaggucc aguacgccgg caccgaugga ccguguaagg ugcccgcaca gauggccguc     1320

gacaugcaaa cacugacccc ugugggcaga cuuauuacug cuaauccagu uaucacggaa     1380

agcacagaga acuccaaaau gaugcuggag uuggaucccc cuuucgggga cucuuauauc     1440

gugauuggag uaggugaaaa gaaaauaacc caccauuggc acaggagcgg cucaacuauc     1500

gggaaggccu uugaggcaac cguccgggga gcgaaacgaa uggcugugcu cggcgauaca     1560

gccugggacu ucgguagugu ugggggcgca uuaaauuccu uaggaaaggg cauucaccag     1620

aucuucgggg ccgcuuuuaa gagccuguuc ggagguaugu cuugguuuuc acagauacua     1680

auuggcaccc uccugaugug gcuuggguug aacgccaaaa acggaaguau cagccugaug     1740

ugccucgcac ugggcggugu gcuuaucuuc cuguccacug cugucucugc c              1791


<210>  9835
<211>  1779
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9835
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcgccguu       60

acucuucccu cccauucuac gaggaagcug cagacccggu cacaaacaug guuggagagu      120

cgcgaauaca cuaaacacuu aauuaaggug gagaacugga uauuucgaaa uccuggcuuc      180

gcgcugguag cuguggccau cgcauggcua cucgggagcu ccaccucuca gaaagucauu      240

uaucugguga ugauccuucu gauugcccca gcuuacucga uccguugcau aggaguuuca      300

aacagagauu uuguggaagg uaugagcggc gggacauggg ucgacguggu auuggagcau      360

ggaggcugug uuaccgugau ggcacaggau aagccgacug ucgacaucga acucgugaca      420

accacgguga guaauauggc cgaggucagg uccuauugcu acgaggcuuc uauuagcgau      480

auggccuccg acucacggug ucccacucag ggugaagcgu aucuggauaa acaaagugac      540

acccaguacg uuugcaagcg cacacuggug gauagagggu ggggaaacgg cuguggccuc      600

uucgggaagg ggagcuuagu aacuugcgca aaauuuaccu guucuaagaa aaugacaggu      660

aaguccaucc agccugagaa ucuggaauau aggauuaugc uuucagugca cggcagccag      720

cauaguggga ugaucggaua cgagaccgac gaggaucggg ccaaagucga agugacgcca      780

aacucucccc gcgcugaggc cacuuugggc gguuucgggu cccugggacu agacugcgaa      840

ccucgaacag gccucgauuu uagcgaccug uacuaucuua ccaugaauaa caagcacugg      900

cugguucaca aggagugguu ccaugacaua ccauugcccu ggcacgcagg cgcugauacu      960

gggacaccuc auuggaauaa caaagaagcc cucguggagu uuaaggacgc gcacgccaaa     1020

agacaaaccg ucgugguacu gggaucgcag gagggugcag uucacacggc uuuagccggc     1080

gcucuggaag ccgagaugga uggggcaaag ggaaggcuuu ucucuggcca ucucaaaugu     1140

cggcugaaga uggacaaauu gcgucuaaag gguguguccu acucacugug caccgccgcu     1200

uuuacauuca cuaagguccc agcggaaacc cuccacggga cagugacugu cgaggugcag     1260

uaugcaggca ccgauggacc guguaaaauu cccguucaga uggccgugga caugcaaaca     1320

cugaccccug uaggccgccu uaucacugcu aauccaguca uuacggaaag uacagagaac     1380

agcaagauga ugcuggaguu ggaucccccu uuuggggacu ccuacaucgu gaucggaguu     1440

ggugauaaaa agauuaccca ucacuggcau agaucuggca gcacuauagg gaaagccuuc     1500

gaagcaaccg ugaggggagc caagcggaug gcuguccucg gcgacacagc cugggauuuc     1560

gguucagugg gaggcguauu uaauagucug ggaaagggca uccaccagau uuucggggca     1620

gcguuuaaau ccuuauucgg agguaugagc ugguuuucuc agauccugau aggcacccua     1680

cucguguggc uggggcuuaa cacuaagaau ggaucaauua guuugacaug ccuggcucuc     1740

ggcgguguua ugaucuuccu gagcacggcc gucuccgca                            1779


<210>  9836
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9836
auggacugga ccuggauccu guuccucgug gccgcugcaa caagagucca cagcgccguu       60

acucuucccu cccauucuac gaggaagcug cagacccggu cacaaacaug guuggagagu      120

cgcgaauaca cuaaacacuu aauucgagug gagaacugga uauuucguaa uccuggcuuc      180

gcgcuggcug ccgcagccau cgcuuggcua cucgggagcu ccaccucuca gaagguaauu      240

uaucugguga ugauccuucu gauugcacca gccuacucga ucagaugcau aggagucuca      300

aacagggauu uuguggaagg uaugagcggc gggacauggg uugacguggu cuuggagcau      360

ggaggcugug ugaccguaau ggcucaggau aaaccgacug uugacaucga acucgugaca      420

accacgguca guaauauggc cgaggugcgg uccuauugcu acgaggcguc uauuagcgau      480

auggcauccg acucacgcug ucccacucag ggugaagccu aucuggauaa gcaaagugac      540

acccaguacg ugugcaaaag aacacugguc gauagggggu ggggaaacgg cuguggccuc      600

uucgggaagg gaagccuggu uacuugcgcu aaguuugccu guucuaaaaa gaugaccggu      660

aaauccaucc agccugagaa ucuggaauau cggauuaugc uuucagugca cggcagccag      720

cauaguggga ugaucguaaa cgacacagga cacgagaccg augagaaucg cgcaaaggug      780

gaaauaacgc caaacucucc ccgagcugag gccacuuugg gcgguuucgg gucccuggga      840

cuagacugcg aaccuagaac aggccucgau uuuagcgacc uguacuaccu uaccaugaau      900

aacaaacacu ggcuggucca uaaggagugg uuccacgaca uuccauugcc cuggcaugcg      960

ggcgccgaua cugggacacc ucacuggaau aacaaggaag cacucgugga guuuaaagac     1020

gcucacgcca agaggcaaac cguugugguc cugggaucgc aggagggugc ugugcauacg     1080

gccuuagcag gcgcccugga agcugagaug gauggggcga aaggacggcu cucuuccggc     1140

caccuuaagu gucgucugaa aauggacaag uugcgccuaa aagguguauc auauagucug     1200

ugcaccgcag ccuucacauu uacuaagauc ccagcugaaa cccuccaugg gacaguuacu     1260

guggaggucc aguacgccgg caccgaugga ccguguaagg ugcccgcaca gauggccguc     1320

gacaugcaaa cacugacccc ugugggcaga cuuauuacug cuaauccagu uaucacggaa     1380

agcacagaga acuccaaaau gaugcuggag uuggaucccc cuuucgggga cucuuauauc     1440

gugauuggag uaggugaaaa gaaaauaacc caccauuggc acaggagcgg cucaacuauc     1500

gggaaggccu uugaggcaac cguccgggga gcgaaacgaa uggcugugcu cggcgauaca     1560

gccugggacu ucgguagugu ugggggcgca uuaaauuccu uaggaaaggg cauucaccag     1620

aucuucgggg ccgcuuuuaa gagccuguuc ggagguaugu cuugguuuuc acagauacua     1680

auuggcaccc uccugaugug gcuuggguug aacacuaaaa acggaaguau cagccugaug     1740

ugccucgccc ugggcggugu gcuuaucuuc cuguccacag cagucucugc u              1791


<210>  9837
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9837
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cugccguuac ccuucccuca cacaguacaa ggaaacugca gacucggagc      120

caaacguggu uggagucccg cgaauacacc aagcauuuaa uacgagugga gaacuggauc      180

uuccguaauc cugguuuugc acuggccgcu gcagccauug cuuggcuacu cgggucuucg      240

acaucacaga aaguaaucua ucuggugaug auucuucuga ucgccccagc guacagcaua      300

agauguaucg gggucaguaa cagggacuuc guggaaggaa uguccggcgg uacuuggguu      360

gauauugugu uggagcacgg gggaugcguc accgugaugg cacaggacaa gccgacagua      420

gauaucgaac ucguuaccac uacagugucu aauauggccg agguccggag cuauuguuac      480

gaggcuucca uuucagacau ggccagugau agccgcugcc ccacccaggg cgaagcauau      540

cuggacaaac aaucugauac gcaguacgug uguaagagaa cucuggugga caggggcugg      600

gggaacggau gcggucucuu uggcaagggg uccuuaguca ccugugcuaa auucgccugc      660

ucaaagaaaa ugacaggaaa gagcauccag ccugagaauc uggaauaucg gauaaugcuu      720

aguguucaug gcucucagca cuccgguaug auugugaacg auacugggca ugagaccgac      780

gagaaucgcg cgaaaguaga aaucacacca aacagccccc gagccgaggc aaccuuggga      840

ggcuuuggcu cgcuggggcu agauugugaa ccuagaacgg gacucgacuu cucugaucug      900

uacuaccuua cuaugaauaa caagcacugg cuggugcaca aggagugguu ucaugacauu      960

ccauugcccu ggcacgcugg ugccgacaca ggcaccccuc auuggaauaa caaagaagcu     1020

cucgucgagu ucaaggaugc ccacgcaaaa aggcaaacug ugguugugcu ggggucccag     1080

gagggagccg uccacacagc uuuagcgggc gcacuggaag ccgagaugga cggugcuaag     1140

gggcggcucu caaguggcca ucuuaaaugc cgucugaaga uggauaaauu gcgccuaaag     1200

ggagugagcu auucccugug uaccgccgca uuuacguuca ccaagauccc agccgaaaca     1260

cuccacggca cuguaaccgu ugaggugcag uacgcuggga cagacggacc gugcaaaguc     1320

cccgcccaga uggcagugga uaugcaaacu cugaccccug ucgguagacu uaucacagcg     1380

aauccaguga uuaccgaauc uacugagaac agcaagauga ugcuggaguu ggaucccccu     1440

uuuggcgacu cauauauagu uaucggggug ggagagaaaa agauuacgca ucacuggcau     1500

aggaguggcu ccacaaucgg uaaggcuuuc gaagccaccg uacggggcgc aaaacgaaug     1560

gccguccucg gcgacacugc uugggauuuu ggaagcgugg gcggggccuu aaauucucug     1620

ggaaagggua uacaccagau uuucggcgca gcuuucaaau cacuguuugg gggaaugagu     1680

ugguucagcc agauccuaau cggcacccuc cugauguggc uugguuugaa cacaaagaac     1740

ggguccauuu cucugaugug ccucgcccug ggcggaguuc uuaucuuucu gucgaccgcg     1800

gugagcgcc                                                             1809


<210>  9838
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9838
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cugccguuac ccuucccuca cacaguacaa ggaaacugca gacucggagc      120

caaacguggu uggagucccg cgaauacacc aagcauuuaa uacgagugga gaacuggauc      180

uuccguaauc cugguuuugc acuggccgcu gcagccauug cuuggcuacu cgggucuucg      240

acaucacaga aaguaaucua ucuggugaug auucuucuga ucgccccagc guacagcaua      300

agauguaucg gggucaguaa cagggacuuc guggaaggaa uguccggcgg uacuuggguu      360

gauguggucu uggagcacgg gggaugcgug accguaaugg cacaggacaa gccgacaguu      420

gauauugaac ucgugaccac uacagucucu aauauggccg aggugcggag cuauuguuac      480

gaggcuucca ucucagacau ggccagugau agccgcugcc ccacccaggg cgaagcauau      540

cuggacaaac aaucugauac gcaguacgug uguaagagaa cucuggucga caggggcugg      600

gggaacggau gcggucucuu uggcaagggg uccuuaguua ccugugcuaa auucgccugc      660

ucaaagaaaa ugacaggaaa gagcauucag ccugagaauc uggaauaucg gaucaugcuu      720

agugugcaug gcucucagca cuccgguaug auaguaaacg auacugggca ugagaccgac      780

gagaaucgcg cgaaagugga aauuacacca aacagccccc gagccgaggc aaccuuggga      840

ggcuuuggcu cgcuggggcu agauugugaa ccuagaacgg gacucgacuu cucugaucug      900

uacuaccuua cuaugaauaa caagcacugg cugguccaca aggagugguu ucaugacauc      960

ccauugcccu ggcacgcugg ugccgacaca ggcaccccuc auuggaauaa caaagaagcu     1020

cucguggagu ucaaggaugc ccacgcaaaa aggcaaacug uugugguccu ggggucccag     1080

gagggagccg ugcacacagc uuuagcgggc gcacuggaag ccgagaugga cggugcuaag     1140

gggcggcucu caaguggcca ucuuaaaugc cgucugaaga uggauaaauu gcgccuaaag     1200

ggaguaagcu auucccugug uaccgccgca uuuacguuca ccaagauucc agccgaaaca     1260

cuccacggca cuguuaccgu ggagguccag uacgcuggga cagacggacc gugcaaagug     1320

cccgcccaga uggcagucga uaugcaaacu cugaccccug uggguagacu uaucacagcg     1380

aauccaguua ucaccgaauc uacugagaac agcaagauga ugcuggaguu ggaucccccu     1440

uuuggcgacu cauauauugu gauaggggua ggagagaaaa agaucacgca ucacuggcau     1500

aggaguggcu ccacaauugg uaaggcuuuc gaagccaccg uccggggcgc aaaacgaaug     1560

gccgugcucg gcgacacugc uugggauuuu ggaagcguug gcggggccuu aaauucucug     1620

ggaaagggua uccaccagau auucggcgca gcuuucaaau cacuguuugg gggaaugagu     1680

ugguucagcc agauucuaau cggcacccuc cugauguggc uugguuugaa cgccaagaac     1740

ggguccaucu cucugaugug ccucgcgcug ggcggagugc uuauuuuucu gucgacagcc     1800

gucagcgcu                                                             1809


<210>  9839
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9839
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cugccguuac ccuucccuca cacaguacaa ggaaacugca gacucggagc      120

caaacguggu uggagucccg cgaauacacc aagcauuuaa uaaaagugga gaacuggauc      180

uuccgaaauc cugguuuugc acugguagcc guggcuauug cauggcuacu cgggucuucg      240

acaucacaga aggucaucua ucuggugaug auucuucuga ucgccccagc uuacagcaua      300

cguuguaucg ggguuaguaa cagagacuuc guggaaggaa uguccggcgg uacuuggguc      360

gaugugguau uggagcacgg gggaugcguu accgugaugg cccaggacaa accgacaguc      420

gauauugaac ucgugaccac uacagugucu aauauggcgg aggucaggag cuauuguuac      480

gaggcaucca ucucagacau ggccagugau agccggugcc ccacccaggg cgaggcuuau      540

cuggacaagc aaucugauac gcaguacguu uguaagcgca cucuggugga cagaggcugg      600

gggaacggau gcggucucuu uggcaaaggg uccuuaguaa ccugugccaa guucacaugc      660

agcaaaaaga ugacuggaaa aucaauucag ccugaaaauc uggaauauag gaucaugcuu      720

agugugcaug gcucucagca cuccgguaug auaggguacg agaccgauga ggaccgggca      780

aaggucgaag ugacaccaaa cagcccccgc gcugaggcca ccuugggagg cuuuggcucg      840

cuggggcuag auugugaacc ucgaacggga cucgacuucu cugaucugua cuaucuuacu      900

augaauaaca agcauuggcu gguucacaaa gagugguuuc acgacauucc auugcccugg      960

caugcgggug ccgacacagg caccccucac uggaauaaca aggaagcacu cguggaguuc     1020

aaagaugcuc augccaagag acaaacuguc gugguacugg ggucccagga gggagcuguu     1080

cacacagccu uagcaggcgc ccuggaagcu gagauggacg gugcgaaagg gaggcucuuu     1140

ucaggccacc uuaagugccg gcugaaaaug gauaaguugc gucuaaaggg agugaguuac     1200

agccugugua ccgcagccuu cacguuuacc aaagucccag cugaaacacu ccauggcacu     1260

gugaccgucg aggugcagua ugccgggaca gacggaccgu gcaagauccc cguucagaug     1320

gcaguggaua ugcaaacucu gaccccugua ggucgccuua uuacagccaa uccagucauc     1380

accgaaucca cugagaacuc uaaaaugaug cuggaguugg aucccccuuu cggcgacagc     1440

uacaucguga uugggguugg agacaagaaa auaacgcacc auuggcacag aucaggcagu     1500

acaaucggua aggcuuuuga agccaccgug aggggcgcaa agcggauggc gguccucggc     1560

gauacugcuu gggacuucgg auccgugggc gggguauuca auagccuggg aaaagguauu     1620

caucagaucu uuggcgccgc auucaagucu uuauuugggg gaaugucaug guucagucag     1680

auacugauug gcacccuacu cguguggcug ggucuuaaca ccaaaaaugg gagcaucucc     1740

uugacaugcc uggcccucgg cggaguuaug aucuuucugu cuacugcugu cucggcc        1797


<210>  9840
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9840
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgcagu ggucauugcc       60

ugcgcgggag cugccguuac ccuucccuca cacaguacaa ggaaacugca gacucggagc      120

caaacguggu uggagucccg cgaauacacc aagcauuuaa uacgagugga gaacuggauc      180

uuccguaauc cugguuuugc acuggccgcu gcagccauug cuuggcuacu cgggucuucg      240

acaucacaga aaguaaucua ucuggugaug auucuucuga ucgccccagc guacagcaua      300

agauguaucg gggucaguaa cagggacuuc guggaaggaa uguccggcgg uacuuggguu      360

gauguggucu uggagcacgg gggaugcgug accguaaugg cacaggacaa gccgacaguu      420

gauauugaac ucgugaccac uacagucucu aauauggccg aggugcggag cuauuguuac      480

gaggcuucca ucucagacau ggccagugau agccgcugcc ccacccaggg cgaagcauau      540

cuggacaaac aaucugauac gcaguacgug uguaagagaa cucuggucga caggggcugg      600

gggaacggau gcggucucuu uggcaagggg uccuuaguua ccugugcuaa auucgccugc      660

ucaaagaaaa ugacaggaaa gagcauucag ccugagaauc uggaauaucg gaucaugcuu      720

agugugcaug gcucucagca cuccgguaug auaguaaacg auacugggca ugagaccgac      780

gagaaucgcg cgaaagugga aauuacacca aacagccccc gagccgaggc aaccuuggga      840

ggcuuuggcu cgcuggggcu agauugugaa ccuagaacgg gacucgacuu cucugaucug      900

uacuaccuua cuaugaauaa caagcacugg cugguccaca aggagugguu ucaugacauc      960

ccauugcccu ggcacgcugg ugccgacaca ggcaccccuc auuggaauaa caaagaagcu     1020

cucguggagu ucaaggaugc ccacgcaaaa aggcaaacug uugugguccu ggggucccag     1080

gagggagccg ugcacacagc uuuagcgggc gcacuggaag ccgagaugga cggugcuaag     1140

gggcggcucu caaguggcca ucuuaaaugc cgucugaaga uggauaaauu gcgccuaaag     1200

ggaguaagcu auucccugug uaccgccgca uuuacguuca ccaagauucc agccgaaaca     1260

cuccacggca cuguuaccgu ggagguccag uacgcuggga cagacggacc gugcaaagug     1320

cccgcccaga uggcagucga uaugcaaacu cugaccccug uggguagacu uaucacagcg     1380

aauccaguua ucaccgaauc uacugagaac agcaagauga ugcuggaguu ggaucccccu     1440

uuuggcgacu cauauauugu gauaggggua ggagagaaaa agaucacgca ucacuggcau     1500

aggaguggcu ccacaauugg uaaggcuuuc gaagccaccg uccggggcgc aaaacgaaug     1560

gccgugcucg gcgacacugc uugggauuuu ggaagcguug gcggggccuu aaauucucug     1620

ggaaagggua uccaccagau auucggcgca gcuuucaaau cacuguuugg gggaaugagu     1680

ugguucagcc agauucuaau cggcacccuc cugauguggc uugguuugaa cacaaagaac     1740

ggguccaucu cucugaugug ccucgcccug ggcggagugc uuauuuuucu gucgaccgcg     1800

gucagcgcc                                                             1809


<210>  9841
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9841
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgcc                     2085


<210>  9842
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9842
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacac cugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacgcc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgcc                     2085


<210>  9843
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9843
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccaucac cagaagaggc agcgccuacu acauguaccu ggacagaagc      120

gacgccggca aggccaucag cuucgccacc acccugggcg ugaacaagug ccacgugcag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucaa gguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

guggccgugg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc accugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg gcuacgagac cgacgaggac agagccaagg uggaggugac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cuguucagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaagg ugcccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa gauccccgug cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgac     1740

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1800

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1860

gugggcggcg uguucaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1920

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcuggug     1980

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ccugccuggc ccugggcggc     2040

gugaugaucu uccugagcac cgccgugagc gcc                                  2073


<210>  9844
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9844
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     2040

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgcc                     2085


<210>  9845
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9845
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1800

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1860

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1920

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1980

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2040

uuccugagca ccgccgugag cgcc                                            2064


<210>  9846
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9846
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1800

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1860

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1920

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1980

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2040

uuccugagca ccgccgugag cgcc                                            2064


<210>  9847
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9847
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcaucacc       60

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      120

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1020

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1080

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1140

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1200

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1260

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1320

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1380

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1440

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1500

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1560

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1620

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1680

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1740

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1800

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1860

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1920

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     1980

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     2040

gccgugagcg cc                                                         2052


<210>  9848
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9848
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1800

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1860

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1920

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1980

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     2040

uuccugagca ccgccgugag cgcc                                            2064


<210>  9849
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9849
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg cc                        2082


<210>  9850
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9850
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaccug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacgccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg cc                        2082


<210>  9851
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9851
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccaucaccag aagaggcagc gccuacuaca uguaccugga cagaagcgac      120

gccggcaagg ccaucagcuu cgccaccacc cugggcguga acaagugcca cgugcagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucaaggu ggagaacugg aucuucagaa accccggcuu cgcccuggug      480

gccguggcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucacc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucggcu acgagaccga cgaggacaga gccaaggugg aggugacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug uucagcggcc accugaagug cagacugaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaagau ccccgugcag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     1740

aagaucaccc accacuggca cagaagcggc agcaccaucg gcaaggccuu cgaggccacc     1800

gugagaggcg ccaagagaau ggccgugcug ggcgacaccg ccugggacuu cggcagcgug     1860

ggcggcgugu ucaacagccu gggcaagggc auccaccaga ucuucggcgc cgccuucaag     1920

agccuguucg gcggcaugag cugguucagc cagauccuga ucggcacccu gcuggugugg     1980

cugggccuga acaccaagaa cggcagcauc agccugaccu gccuggcccu gggcggcgug     2040

augaucuucc ugagcaccgc cgugagcgcc                                      2070


<210>  9852
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9852
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc     1800

uucgaggcca ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac     1860

uucggcagcg ugggcggcgc ccugaacagc cugggcaagg gcauccacca gaucuucggc     1920

gccgccuuca agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc     1980

cugcugaugu ggcugggccu gaacaccaag aacggcagca ucagccugau gugccuggcc     2040

cugggcggcg ugcugaucuu ccugagcacc gccgugagcg cc                        2082


<210>  9853
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9853
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac ccugggcaag     1800

gccuucagca ccacccugaa gggcgcccag agacuggccg cccugggcga caccgccugg     1860

gacuucggca gcaucggcgg cguguucaac agcaucggca gagccgugca ccagguguuc     1920

ggcggcgccu ucagaacccu guucggcggc augagcugga ucacccaggg ccugaugggc     1980

gcccugcugc uguggauggg cgugaacgcc agagacagaa gcaucgcccu ggccuuccug     2040

gccaccggcg gcgugcuggu guuccuggcc accaacgugc acgcc                     2085


<210>  9854
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9854
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacac cugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac ccugggcaag     1800

gccuucagca ccacccugaa gggcgcccag agacuggccg cccugggcga caccgccugg     1860

gacuucggca gcaucggcgg cguguucaac agcaucggca gagccgugca ccagguguuc     1920

ggcggcgccu ucagaacccu guucggcggc augagcugga ucacccaggg ccugaugggc     1980

gcccugcugc uguggauggg cgugaacgcc agagacagaa gcaucgcccu ggccuuccug     2040

gccaccggcg gcgugcuggu guuccuggcc accaacgugc acgcc                     2085


<210>  9855
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9855
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccaucac cagaagaggc agcgccuacu acauguaccu ggacagaagc      120

gacgccggca aggccaucag cuucgccacc acccugggcg ugaacaagug ccacgugcag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucaa gguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

guggccgugg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc accugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg gcuacgagac cgacgaggac agagccaagg uggaggugac ccccaacagc     1080

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga     1140

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug     1200

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc     1260

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1320

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1380

gaggccgaga uggacggcgc caagggcaga cuguucagcg gccaccugaa gugcagacug     1440

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1500

uucaccaagg ugcccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1560

ggcaccgacg gccccugcaa gauccccgug cagauggccg uggacaugca gacccugacc     1620

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1680

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgac     1740

aagaagauca cccaccacug gcacagaagc ggcagcaccc ugggcaaggc cuucagcacc     1800

acccugaagg gcgcccagag acuggccgcc cugggcgaca ccgccuggga cuucggcagc     1860

aucggcggcg uguucaacag caucggcaga gccgugcacc agguguucgg cggcgccuuc     1920

agaacccugu ucggcggcau gagcuggauc acccagggcc ugaugggcgc ccugcugcug     1980

uggaugggcg ugaacgccag agacagaagc aucgcccugg ccuuccuggc caccggcggc     2040

gugcuggugu uccuggccac caacgugcac gcc                                  2073


<210>  9856
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9856
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac      120

gacgccggcg aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag      180

aucauggacc ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac      240

gagggcgugg agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug      300

uacggcaccu gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug      360

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      420

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      480

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      540

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      600

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      660

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      720

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      780

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      840

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      900

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      960

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc     1020

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac ccugggcaag     1800

gccuucagca ccacccugaa gggcgcccag agacuggccg cccugggcga caccgccugg     1860

gacuucggca gcaucggcgg cguguucaac agcaucggca gagccgugca ccagguguuc     1920

ggcggcgccu ucagaacccu guucggcggc augagcugga ucacccaggg ccugaugggc     1980

gcccugcugc uguggauggg cgugaacgcc agagacagaa gcaucgcccu ggccuuccug     2040

gccaccggcg gcgugcuggu guuccuggcc accaacgugc acgcc                     2085


<210>  9857
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9857
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc cugggcaagg ccuucagcac cacccugaag     1800

ggcgcccaga gacuggccgc ccugggcgac accgccuggg acuucggcag caucggcggc     1860

guguucaaca gcaucggcag agccgugcac cagguguucg gcggcgccuu cagaacccug     1920

uucggcggca ugagcuggau cacccagggc cugaugggcg cccugcugcu guggaugggc     1980

gugaacgcca gagacagaag caucgcccug gccuuccugg ccaccggcgg cgugcuggug     2040

uuccuggcca ccaacgugca cgcc                                            2064


<210>  9858
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9858
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacacc      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc cugggcaagg ccuucagcac cacccugaag     1800

ggcgcccaga gacuggccgc ccugggcgac accgccuggg acuucggcag caucggcggc     1860

guguucaaca gcaucggcag agccgugcac cagguguucg gcggcgccuu cagaacccug     1920

uucggcggca ugagcuggau cacccagggc cugaugggcg cccugcugcu guggaugggc     1980

gugaacgcca gagacagaag caucgcccug gccuuccugg ccaccggcgg cgugcuggug     2040

uuccuggcca ccaacgugca cgcc                                            2064


<210>  9859
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9859
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcaucacc       60

agaagaggca gcgccuacua cauguaccug gacagaagcg acgccggcaa ggccaucagc      120

uucgccacca cccugggcgu gaacaagugc cacgugcaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc     1020

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug     1080

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac     1140

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac     1200

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1260

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1320

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1380

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1440

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1500

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1560

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1620

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1680

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1740

cacagaagcg gcagcacccu gggcaaggcc uucagcacca cccugaaggg cgcccagaga     1800

cuggccgccc ugggcgacac cgccugggac uucggcagca ucggcggcgu guucaacagc     1860

aucggcagag ccgugcacca gguguucggc ggcgccuuca gaacccuguu cggcggcaug     1920

agcuggauca cccagggccu gaugggcgcc cugcugcugu ggaugggcgu gaacgccaga     1980

gacagaagca ucgcccuggc cuuccuggcc accggcggcg ugcugguguu ccuggccacc     2040

aacgugcacg cc                                                         2052


<210>  9860
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9860
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgugacc       60

agaagaggca gcgccuacua cauguaccug gacagaaacg acgccggcga ggccaucagc      120

uuccccacca cccugggcau gaacaagugc uacauccaga ucauggaccu gggccacaug      180

ugcgacgcca ccaugagcua cgagugcccc augcuggacg agggcgugga gcccgacgac      240

guggacugcu ggugcaacac caccagcacc uggguggugu acggcaccug ccaccacaag      300

aagggcgagg ccagaagaag cagaagagcc gugacccugc ccagccacag caccagaaag      360

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      420

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      480

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      540

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      600

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      660

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      720

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      780

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      840

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      900

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      960

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc     1020

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc     1080

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug     1140

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag     1200

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1260

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1320

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1380

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1440

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1500

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1560

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1620

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1680

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1740

acccaccacu ggcacagaag cggcagcacc cugggcaagg ccuucagcac cacccugaag     1800

ggcgcccaga gacuggccgc ccugggcgac accgccuggg acuucggcag caucggcggc     1860

guguucaaca gcaucggcag agccgugcac cagguguucg gcggcgccuu cagaacccug     1920

uucggcggca ugagcuggau cacccagggc cugaugggcg cccugcugcu guggaugggc     1980

gugaacgcca gagacagaag caucgcccug gccuuccugg ccaccggcgg cgugcuggug     2040

uuccuggcca ccaacgugca cgcc                                            2064


<210>  9861
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9861
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacaucg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcacccu gggcaaggcc     1800

uucagcacca cccugaaggg cgcccagaga cuggccgccc ugggcgacac cgccugggac     1860

uucggcagca ucggcggcgu guucaacagc aucggcagag ccgugcacca gguguucggc     1920

ggcgccuuca gaacccuguu cggcggcaug agcuggauca cccagggccu gaugggcgcc     1980

cugcugcugu ggaugggcgu gaacgccaga gacagaagca ucgcccuggc cuuccuggcc     2040

accggcggcg ugcugguguu ccuggccacc aacgugcacg cc                        2082


<210>  9862
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9862
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaccug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcacccu gggcaaggcc     1800

uucagcacca cccugaaggg cgcccagaga cuggccgccc ugggcgacac cgccugggac     1860

uucggcagca ucggcggcgu guucaacagc aucggcagag ccgugcacca gguguucggc     1920

ggcgccuuca gaacccuguu cggcggcaug agcuggauca cccagggccu gaugggcgcc     1980

cugcugcugu ggaugggcgu gaacgccaga gacagaagca ucgcccuggc cuuccuggcc     2040

accggcggcg ugcugguguu ccuggccacc aacgugcacg cc                        2082


<210>  9863
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9863
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccaucaccag aagaggcagc gccuacuaca uguaccugga cagaagcgac      120

gccggcaagg ccaucagcuu cgccaccacc cugggcguga acaagugcca cgugcagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucaaggu ggagaacugg aucuucagaa accccggcuu cgcccuggug      480

gccguggcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucacc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucggcu acgagaccga cgaggacaga gccaaggugg aggugacccc caacagcccc     1080

agagccgagg ccacccuggg cggcuucggc agccugggcc uggacugcga gcccagaacc     1140

ggccuggacu ucagcgaccu guacuaccug accaugaaca acaagcacug gcuggugcac     1200

aaggaguggu uccacgacau cccccugccc uggcacgccg gcgccgacac cggcaccccc     1260

cacuggaaca acaaggaggc ccugguggag uucaaggacg cccacgccaa gagacagacc     1320

gugguggugc ugggcagcca ggagggcgcc gugcacaccg cccuggccgg cgcccuggag     1380

gccgagaugg acggcgccaa gggcagacug uucagcggcc accugaagug cagacugaag     1440

auggacaagc ugagacugaa gggcgugagc uacagccugu gcaccgccgc cuucaccuuc     1500

accaaggugc ccgccgagac ccugcacggc accgugaccg uggaggugca guacgccggc     1560

accgacggcc ccugcaagau ccccgugcag auggccgugg acaugcagac ccugaccccc     1620

gugggcagac ugaucaccgc caaccccgug aucaccgaga gcaccgagaa cagcaagaug     1680

augcuggagc uggacccccc cuucggcgac agcuacaucg ugaucggcgu gggcgacaag     1740

aagaucaccc accacuggca cagaagcggc agcacccugg gcaaggccuu cagcaccacc     1800

cugaagggcg cccagagacu ggccgcccug ggcgacaccg ccugggacuu cggcagcauc     1860

ggcggcgugu ucaacagcau cggcagagcc gugcaccagg uguucggcgg cgccuucaga     1920

acccuguucg gcggcaugag cuggaucacc cagggccuga ugggcgcccu gcugcugugg     1980

augggcguga acgccagaga cagaagcauc gcccuggccu uccuggccac cggcggcgug     2040

cugguguucc uggccaccaa cgugcacgcc                                      2070


<210>  9864
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9864
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgugaccag aagaggcagc gccuacuaca uguaccugga cagaaacgac      120

gccggcgagg ccaucagcuu ccccaccacc cugggcauga acaagugcua cauccagauc      180

auggaccugg gccacaugug cgacgccacc augagcuacg agugccccau gcuggacgag      240

ggcguggagc ccgacgacgu ggacugcugg ugcaacacca ccagcaccug ggugguguac      300

ggcaccugcc accacaagaa gggcgaggcc agaagaagca gaagagccgu gacccugccc      360

agccacagca ccagaaagcu gcagaccaga agccagaccu ggcuggagag cagagaguac      420

accaagcacc ugaucagagu ggagaacugg aucuucagaa accccggcuu cgcccuggcc      480

gccgccgcca ucgccuggcu gcugggcagc agcaccagcc agaaggugau cuaccuggug      540

augauccugc ugaucgcccc cgccuacagc aucagaugca ucggcgugag caacagagac      600

uucguggagg gcaugagcgg cggcaccugg guggacgugg ugcuggagca cggcggcugc      660

gugaccguga uggcccagga caagcccacc guggacaucg agcuggugac caccaccgug      720

agcaacaugg ccgaggugag aagcuacugc uacgaggcca gcaucagcga cauggccagc      780

gacagcagau gccccaccca gggcgaggcc uaccuggaca agcagagcga cacccaguac      840

gugugcaaga gaacccuggu ggacagaggc uggggcaacg gcugcggccu guucggcaag      900

ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga agaugaccgg caagagcauc      960

cagcccgaga accuggagua cagaaucaug cugagcgugc acggcagcca gcacagcggc     1020

augaucguga acgacaccgg ccacgagacc gacgagaaca gagccaaggu ggagaucacc     1080

cccaacagcc ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc     1140

gagcccagaa ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac     1200

uggcuggugc acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac     1260

accggcaccc cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc     1320

aagagacaga ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc     1380

ggcgcccugg aggccgagau ggacggcgcc aagggcagac ugagcagcgg ccaccugaag     1440

ugcagacuga agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc     1500

gccuucaccu ucaccaagau ccccgccgag acccugcacg gcaccgugac cguggaggug     1560

caguacgccg gcaccgacgg ccccugcaag gugcccgccc agauggccgu ggacaugcag     1620

acccugaccc ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag     1680

aacagcaaga ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc     1740

gugggcgaga agaagaucac ccaccacugg cacagaagcg gcagcacccu gggcaaggcc     1800

uucagcacca cccugaaggg cgcccagaga cuggccgccc ugggcgacac cgccugggac     1860

uucggcagca ucggcggcgu guucaacagc aucggcagag ccgugcacca gguguucggc     1920

ggcgccuuca gaacccuguu cggcggcaug agcuggauca cccagggccu gaugggcgcc     1980

cugcugcugu ggaugggcgu gaacgccaga gacagaagca ucgcccuggc cuuccuggcc     2040

accggcggcg ugcugguguu ccuggccacc aacgugcacg cc                        2082


<210>  9865
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9865
augaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgugacccug       60

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      120

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      180

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      240

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      300

gacuucgugg agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc      360

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      420

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      480

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      540

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      600

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      660

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc      720

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc      780

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac      840

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag      900

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc      960

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1020

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1080

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1140

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1200

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1260

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1320

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1380

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1440

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1500

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1560

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1620

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1680

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     1740

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgcc                     1785


<210>  9866
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9866
augaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgugacccug       60

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      120

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      180

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      240

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      300

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      360

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      420

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      480

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      540

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      600

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      660

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc      720

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc      780

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac      840

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag      900

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc      960

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1020

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1080

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1140

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1200

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1260

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1320

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1380

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1440

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1500

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1560

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1620

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1680

acccugcuga uguggcuggg ccugaacgcc aagaacggca gcaucagccu gaugugccug     1740

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgcc                     1785


<210>  9867
<211>  1773
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9867
augaccagca ucggcaucau cggccugcug cugaccaccg ccauggccgc cgugacccug       60

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      120

uacaccaagc accugaucaa gguggagaac uggaucuuca gaaaccccgg cuucgcccug      180

guggccgugg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      240

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      300

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      360

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      420

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      480

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      540

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      600

aagggcagcc uggugaccug cgccaaguuc accugcagca agaagaugac cggcaagagc      660

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc      720

ggcaugaucg gcuacgagac cgacgaggac agagccaagg uggaggugac ccccaacagc      780

cccagagccg aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga      840

accggccugg acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug      900

cacaaggagu gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc      960

ccccacugga acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag     1020

accguggugg ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug     1080

gaggccgaga uggacggcgc caagggcaga cuguucagcg gccaccugaa gugcagacug     1140

aagauggaca agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc     1200

uucaccaagg ugcccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc     1260

ggcaccgacg gccccugcaa gauccccgug cagauggccg uggacaugca gacccugacc     1320

cccgugggca gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag     1380

augaugcugg agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgac     1440

aagaagauca cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc     1500

accgugagag gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc     1560

gugggcggcg uguucaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc     1620

aagagccugu ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcuggug     1680

uggcugggcc ugaacaccaa gaacggcagc aucagccuga ccugccuggc ccugggcggc     1740

gugaugaucu uccugagcac cgccgugagc gcc                                  1773


<210>  9868
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9868
augaccagcg ugggcaucgu gggccugcug cugaccaccg ccauggccgc cgugacccug       60

cccagccaca gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag      120

uacaccaagc accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug      180

gccgccgccg ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug      240

gugaugaucc ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga      300

gacuucgugg agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc      360

ugcgugaccg ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc      420

gugagcaaca uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc      480

agcgacagca gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag      540

uacgugugca agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc      600

aagggcagcc uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc      660

auccagcccg agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc      720

ggcaugaucg ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc      780

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac      840

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag      900

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc      960

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1020

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1080

gccggcgccc uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug     1140

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1200

gccgccuuca ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag     1260

gugcaguacg ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug     1320

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1380

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1440

ggcgugggcg agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1500

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1560

gacuucggca gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc     1620

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1680

acccugcuga uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug     1740

gcccugggcg gcgugcugau cuuccugagc accgccguga gcgcc                     1785


<210>  9869
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9869
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      120

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      180

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      240

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      300

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      360

guggacaucg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      420

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      480

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      540

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      600

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      660

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug      720

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc      780

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug      840

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      900

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      960

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1020

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1080

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1140

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1200

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1260

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1320

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1380

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1440

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1500

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1560

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1620

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1680

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     1740

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     1800

gccgugagcg cc                                                         1812


<210>  9870
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9870
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      120

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      180

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      240

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      300

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      360

guggacgugg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      420

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      480

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      540

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      600

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      660

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug      720

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc      780

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug      840

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      900

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      960

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1020

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1080

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1140

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1200

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1260

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1320

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1380

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1440

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1500

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1560

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1620

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1680

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag     1740

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     1800

gccgugagcg cc                                                         1812


<210>  9871
<211>  1800
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9871
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      120

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg      180

aucuucagaa accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc      240

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      300

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      360

guggacgugg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      420

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      480

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      540

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      600

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc      660

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug      720

cugagcgugc acggcagcca gcacagcggc augaucggcu acgagaccga cgaggacaga      780

gccaaggugg aggugacccc caacagcccc agagccgagg ccacccuggg cggcuucggc      840

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug      900

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc      960

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1020

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1080

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1140

uucagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1200

uacagccugu gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc     1260

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag     1320

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1380

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1440

agcuacaucg ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc     1500

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1560

ggcgacaccg ccugggacuu cggcagcgug ggcggcgugu ucaacagccu gggcaagggc     1620

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1680

cagauccuga ucggcacccu gcuggugugg cugggccuga acaccaagaa cggcagcauc     1740

agccugaccu gccuggcccu gggcggcgug augaucuucc ugagcaccgc cgugagcgcc     1800


<210>  9872
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9872
auggccauca gcggcgugcc cgugcugggc uucuucauca ucgccgugcu gaugagcgcc       60

caggagagcu gggccgccgu gacccugccc agccacagca ccagaaagcu gcagaccaga      120

agccagaccu ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg      180

aucuucagaa accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc      240

agcaccagcc agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc      300

aucagaugca ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg      360

guggacgugg ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc      420

guggacaucg agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc      480

uacgaggcca gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc      540

uaccuggaca agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc      600

uggggcaacg gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc      660

ugcagcaaga agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug      720

cugagcgugc acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc      780

gacgagaaca gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug      840

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      900

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      960

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1020

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1080

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1140

aagggcagac ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1200

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag     1260

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1320

gugcccgccc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1380

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1440

cccuucggcg acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg     1500

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1560

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc     1620

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1680

agcugguuca gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag     1740

aacggcagca ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc     1800

gccgugagcg cc                                                         1812


<210>  9873
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9873
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgccgug       60

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      120

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      180

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      240

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      300

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacaucgu gcuggagcac      360

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      420

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      480

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      540

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      600

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      660

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag      720

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug      780

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc      840

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac      900

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc      960

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1020

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1080

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1140

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1200

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1260

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1320

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1380

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1440

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1500

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1560

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1620

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1680

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     1740

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc c              1791


<210>  9874
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9874
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgccgug       60

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      120

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      180

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      240

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      300

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacguggu gcuggagcac      360

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      420

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      480

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      540

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      600

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      660

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag      720

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug      780

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc      840

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac      900

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc      960

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1020

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1080

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1140

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1200

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1260

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1320

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1380

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1440

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1500

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1560

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1620

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1680

aucggcaccc ugcugaugug gcugggccug aacgccaaga acggcagcau cagccugaug     1740

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc c              1791


<210>  9875
<211>  1779
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9875
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgccgug       60

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      120

agagaguaca ccaagcaccu gaucaaggug gagaacugga ucuucagaaa ccccggcuuc      180

gcccuggugg ccguggccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      240

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      300

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacguggu gcuggagcac      360

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      420

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      480

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      540

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      600

uucggcaagg gcagccuggu gaccugcgcc aaguucaccu gcagcaagaa gaugaccggc      660

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag      720

cacagcggca ugaucggcua cgagaccgac gaggacagag ccaaggugga ggugaccccc      780

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag      840

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg      900

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc      960

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1020

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1080

gcccuggagg ccgagaugga cggcgccaag ggcagacugu ucagcggcca ccugaagugc     1140

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1200

uucaccuuca ccaaggugcc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1260

uacgccggca ccgacggccc cugcaagauc cccgugcaga uggccgugga caugcagacc     1320

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1380

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1440

ggcgacaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1500

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1560

ggcagcgugg gcggcguguu caacagccug ggcaagggca uccaccagau cuucggcgcc     1620

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1680

cugguguggc ugggccugaa caccaagaac ggcagcauca gccugaccug ccuggcccug     1740

ggcggcguga ugaucuuccu gagcaccgcc gugagcgcc                            1779


<210>  9876
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9876
auggacugga ccuggauccu guuccuggug gccgccgcca ccagagugca cagcgccgug       60

acccugccca gccacagcac cagaaagcug cagaccagaa gccagaccug gcuggagagc      120

agagaguaca ccaagcaccu gaucagagug gagaacugga ucuucagaaa ccccggcuuc      180

gcccuggccg ccgccgccau cgccuggcug cugggcagca gcaccagcca gaaggugauc      240

uaccugguga ugauccugcu gaucgccccc gccuacagca ucagaugcau cggcgugagc      300

aacagagacu ucguggaggg caugagcggc ggcaccuggg uggacguggu gcuggagcac      360

ggcggcugcg ugaccgugau ggcccaggac aagcccaccg uggacaucga gcuggugacc      420

accaccguga gcaacauggc cgaggugaga agcuacugcu acgaggccag caucagcgac      480

auggccagcg acagcagaug ccccacccag ggcgaggccu accuggacaa gcagagcgac      540

acccaguacg ugugcaagag aacccuggug gacagaggcu ggggcaacgg cugcggccug      600

uucggcaagg gcagccuggu gaccugcgcc aaguucgccu gcagcaagaa gaugaccggc      660

aagagcaucc agcccgagaa ccuggaguac agaaucaugc ugagcgugca cggcagccag      720

cacagcggca ugaucgugaa cgacaccggc cacgagaccg acgagaacag agccaaggug      780

gagaucaccc ccaacagccc cagagccgag gccacccugg gcggcuucgg cagccugggc      840

cuggacugcg agcccagaac cggccuggac uucagcgacc uguacuaccu gaccaugaac      900

aacaagcacu ggcuggugca caaggagugg uuccacgaca ucccccugcc cuggcacgcc      960

ggcgccgaca ccggcacccc ccacuggaac aacaaggagg cccuggugga guucaaggac     1020

gcccacgcca agagacagac cgugguggug cugggcagcc aggagggcgc cgugcacacc     1080

gcccuggccg gcgcccugga ggccgagaug gacggcgcca agggcagacu gagcagcggc     1140

caccugaagu gcagacugaa gauggacaag cugagacuga agggcgugag cuacagccug     1200

ugcaccgccg ccuucaccuu caccaagauc cccgccgaga cccugcacgg caccgugacc     1260

guggaggugc aguacgccgg caccgacggc cccugcaagg ugcccgccca gauggccgug     1320

gacaugcaga cccugacccc cgugggcaga cugaucaccg ccaaccccgu gaucaccgag     1380

agcaccgaga acagcaagau gaugcuggag cuggaccccc ccuucggcga cagcuacauc     1440

gugaucggcg ugggcgagaa gaagaucacc caccacuggc acagaagcgg cagcaccauc     1500

ggcaaggccu ucgaggccac cgugagaggc gccaagagaa uggccgugcu gggcgacacc     1560

gccugggacu ucggcagcgu gggcggcgcc cugaacagcc ugggcaaggg cauccaccag     1620

aucuucggcg ccgccuucaa gagccuguuc ggcggcauga gcugguucag ccagauccug     1680

aucggcaccc ugcugaugug gcugggccug aacaccaaga acggcagcau cagccugaug     1740

ugccuggccc ugggcggcgu gcugaucuuc cugagcaccg ccgugagcgc c              1791


<210>  9877
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9877
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      120

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      180

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      240

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      300

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      360

gacaucgugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      420

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      480

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      540

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      600

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      660

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug      720

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac      780

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc      840

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug      900

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc      960

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1020

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1080

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1140

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1200

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1260

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1320

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1380

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1440

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1500

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1560

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1620

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1680

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     1740

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     1800

gugagcgcc                                                             1809


<210>  9878
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9878
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      120

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      180

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      240

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      300

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      360

gacguggugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      420

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      480

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      540

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      600

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      660

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug      720

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac      780

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc      840

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug      900

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc      960

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1020

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1080

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1140

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1200

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1260

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1320

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1380

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1440

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1500

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1560

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1620

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1680

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa cgccaagaac     1740

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     1800

gugagcgcc                                                             1809


<210>  9879
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9879
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      120

cagaccuggc uggagagcag agaguacacc aagcaccuga ucaaggugga gaacuggauc      180

uucagaaacc ccggcuucgc ccugguggcc guggccaucg ccuggcugcu gggcagcagc      240

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      300

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      360

gacguggugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      420

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      480

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      540

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      600

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucaccugc      660

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug      720

agcgugcacg gcagccagca cagcggcaug aucggcuacg agaccgacga ggacagagcc      780

aagguggagg ugacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc      840

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc      900

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg      960

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1020

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1080

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacuguuc     1140

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1200

agccugugca ccgccgccuu caccuucacc aaggugcccg ccgagacccu gcacggcacc     1260

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaagauccc cgugcagaug     1320

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1380

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1440

uacaucguga ucggcguggg cgacaagaag aucacccacc acuggcacag aagcggcagc     1500

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1560

gacaccgccu gggacuucgg cagcgugggc ggcguguuca acagccuggg caagggcauc     1620

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1680

auccugaucg gcacccugcu gguguggcug ggccugaaca ccaagaacgg cagcaucagc     1740

cugaccugcc uggcccuggg cggcgugaug aucuuccuga gcaccgccgu gagcgcc        1797


<210>  9880
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9880
augggcaaga gaagcgccgg cagcaucaug uggcuggcca gccuggccgu ggugaucgcc       60

ugcgccggcg ccgccgugac ccugcccagc cacagcacca gaaagcugca gaccagaagc      120

cagaccuggc uggagagcag agaguacacc aagcaccuga ucagagugga gaacuggauc      180

uucagaaacc ccggcuucgc ccuggccgcc gccgccaucg ccuggcugcu gggcagcagc      240

accagccaga aggugaucua ccuggugaug auccugcuga ucgcccccgc cuacagcauc      300

agaugcaucg gcgugagcaa cagagacuuc guggagggca ugagcggcgg caccugggug      360

gacguggugc uggagcacgg cggcugcgug accgugaugg cccaggacaa gcccaccgug      420

gacaucgagc uggugaccac caccgugagc aacauggccg aggugagaag cuacugcuac      480

gaggccagca ucagcgacau ggccagcgac agcagaugcc ccacccaggg cgaggccuac      540

cuggacaagc agagcgacac ccaguacgug ugcaagagaa cccuggugga cagaggcugg      600

ggcaacggcu gcggccuguu cggcaagggc agccugguga ccugcgccaa guucgccugc      660

agcaagaaga ugaccggcaa gagcauccag cccgagaacc uggaguacag aaucaugcug      720

agcgugcacg gcagccagca cagcggcaug aucgugaacg acaccggcca cgagaccgac      780

gagaacagag ccaaggugga gaucaccccc aacagcccca gagccgaggc cacccugggc      840

ggcuucggca gccugggccu ggacugcgag cccagaaccg gccuggacuu cagcgaccug      900

uacuaccuga ccaugaacaa caagcacugg cuggugcaca aggagugguu ccacgacauc      960

ccccugcccu ggcacgccgg cgccgacacc ggcacccccc acuggaacaa caaggaggcc     1020

cugguggagu ucaaggacgc ccacgccaag agacagaccg ugguggugcu gggcagccag     1080

gagggcgccg ugcacaccgc ccuggccggc gcccuggagg ccgagaugga cggcgccaag     1140

ggcagacuga gcagcggcca ccugaagugc agacugaaga uggacaagcu gagacugaag     1200

ggcgugagcu acagccugug caccgccgcc uucaccuuca ccaagauccc cgccgagacc     1260

cugcacggca ccgugaccgu ggaggugcag uacgccggca ccgacggccc cugcaaggug     1320

cccgcccaga uggccgugga caugcagacc cugacccccg ugggcagacu gaucaccgcc     1380

aaccccguga ucaccgagag caccgagaac agcaagauga ugcuggagcu ggaccccccc     1440

uucggcgaca gcuacaucgu gaucggcgug ggcgagaaga agaucaccca ccacuggcac     1500

agaagcggca gcaccaucgg caaggccuuc gaggccaccg ugagaggcgc caagagaaug     1560

gccgugcugg gcgacaccgc cugggacuuc ggcagcgugg gcggcgcccu gaacagccug     1620

ggcaagggca uccaccagau cuucggcgcc gccuucaaga gccuguucgg cggcaugagc     1680

ugguucagcc agauccugau cggcacccug cugauguggc ugggccugaa caccaagaac     1740

ggcagcauca gccugaugug ccuggcccug ggcggcgugc ugaucuuccu gagcaccgcc     1800

gugagcgcc                                                             1809


<210>  9881
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9881
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggugac cagacgggga agcgcuuacu auauguaccu ggaccgcaac      120

gaugccggcg aagccauuuc cuucccuaca acgcuuggga ugaauaagug cuauauccaa      180

auuauggacc ugggacacau gugugaugcu accaugucuu acgagugccc gaugcucgac      240

gaaggcgugg agcccgauga cgucgauugu uggugcaaca caaccagcac augggugguu      300

uaugggaccu gucaucacaa aaagggagaa gccagacggu cccgcagagc ugugacacug      360

ccuucucaua gcacccggaa acuucagacg cgcucccaga ccuggcugga gagcagagag      420

uacacaaagc accucauccg ggucgaaaau uggaucuuuc gcaaccccgg cuucgcccug      480

gcggccgcug ccauugcuug gcugcucggg ucuuccacca gccagaaagu gaucuaucug      540

guuaugauuc uucugaucgc cccugcguac ucuauuagau gcaucggggu guccaaucgg      600

gacuuugucg agggaaugag cggcgggaca uggguggaua uugugcucga acauggaggc      660

ugugucaccg uuauggccca agacaagccc acgguggaua ucgagcuggu gacaaccaca      720

gucucuaaca uggcugaggu gcgcuccuac ugcuaugaag ccagcaucuc cgacauggcu      780

ucugauagca gauguccuac ccagggggag gccuaccuug acaagcaguc cgacacccag      840

uauguuugca aacggacacu gguggaucgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggucacgug cgccaaauuu gcuugcucua agaaaaugac cgggaagucc      960

auucaaccgg aaaaccugga guacagaauc augcucagcg ugcacggauc ucagcacagc     1020

ggcaugauug uuaaugacac agggcaugaa accgaugaga accgggcgaa gguggagauc     1080

acacccaauu ccccuagagc cgaagcuacc cugggaggcu ucggcucucu ugggcuggac     1140

ugugagcccc gcacgggacu cgauuuuagc gaccuguauu accuuacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gauauuccuc uccccuggca cgccggcgcc     1260

gacaccggga ccccgcauug gaacaauaaa gaggcucugg uggaauuuaa ggaugcccac     1320

gcgaaacggc agacagucgu gguucuggga ucccaggagg gcgcugugca uaccgcccuc     1380

gccggggcuc uggaggccga aauggacggc gcuaagggaa gacuuagcuc uggccaccug     1440

aaaugccgcc ucaagaugga uaagcugcgg cuuaaagggg ucuccuauag ccuguguaca     1500

gccgcguuca cguuuaccaa gaucccugcc gagacacucc auggaaccgu gacaguugaa     1560

gugcaauacg cuggcaccga cgggcccugc aaagucccug cccagauggc uguggacaug     1620

cagacgcuga cccccguggg aagacugauc acagccaacc ccguuauuac cgagucuaca     1680

gaaaacucca agaugaugcu cgagcuggau ccuccguucg gcgacagcua caucgucauu     1740

ggggugggcg agaaaaagau cacccaccac uggcaucgcu ccggaucuac gauuggcaag     1800

gcguucgaag ccacagugcg gggcgcuaaa agaauggccg uccuuggaga uaccgcuugg     1860

gacuuuggca gcguuggggg agcccucaau ucccucggca aggggaucca ccaaaucuuc     1920

ggcgccgcuu uuaaaucucu guucggaggc augagcuggu uuucccagau ucugaucggg     1980

acacuucuca uguggcuggg acugaacacc aagaauggca gcauuucucu caugugucug     2040

gcgcuugggg gagugcugau cuuccucucc accgccguga gcgcu                     2085


<210>  9882
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9882
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggugac cagacgggga agcgcuuacu auauguaccu ggaccgcaac      120

gaugccggcg aagccauuuc cuucccuaca acgcuuggga ugaauaagug cuauauccaa      180

auuauggacc ugggacacac cugugaugcu acaaugucuu acgagugccc gaugcucgac      240

gaaggcgugg agcccgauga cgucgauugu uggugcaaca ccacaagcac cugggugguu      300

uaugggacau gucaucacaa aaagggagaa gccagacggu cccgcagagc ugugacccug      360

ccuucucaua gcacgcggaa acuucagacc cgcucccaga cauggcugga gagcagagag      420

uacaccaagc accucauccg ggucgaaaau uggaucuuuc gcaaccccgg cuucgcccug      480

gcggccgcug ccauugcuug gcugcucggg ucuuccacaa gccagaaagu gaucuaucug      540

guuaugauuc uucugaucgc cccugcguac ucuauuagau gcaucggggu guccaaucgg      600

gacuuugucg agggaaugag cggcgggacc uggguggaug ugguccucga acauggaggc      660

uguguuacgg ugauggccca agacaagccc acaguggaua uugagcuggu caccacaacc      720

gugucuaaca uggcugaggu ucgcuccuac ugcuaugaag ccagcaucuc cgacauggcu      780

ucugauagca gauguccuac ccagggggag gccuaccuug acaagcaguc cgacacacag      840

uaugugugca aacggacgcu ggucgaucgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucua agaaaaugac agggaagucc      960

auccaaccgg aaaaccugga guacagaauu augcucagcg uucacggauc ucagcacagc     1020

ggcaugaucg ugaaugacac cgggcaugaa acagaugaga accgggcgaa ggucgagauu     1080

acccccaauu ccccuagagc cgaagcuacg cugggaggcu ucggcucucu ugggcuggac     1140

ugugagcccc gcacaggacu cgauuuuagc gaccuguauu accuuaccau gaacaauaaa     1200

cacuggcugg ugcauaagga augguuccac gauaucccuc uccccuggca cgccggcgcc     1260

gacaccggga caccgcauug gaacaauaaa gaggcucugg ucgaauuuaa ggaugcccac     1320

gcgaaacggc agaccguggu ugugcuggga ucccaggagg gcgcugucca uacagcccuc     1380

gccggggcuc uggaggccga aauggacggc gcuaagggaa gacuuagcuc uggccaccug     1440

aaaugccgcc ucaagaugga uaagcugcgg cuuaaagggg uguccuauag ccuguguacg     1500

gccgcguuca ccuuuacaaa gauuccugcc gagacccucc auggaacagu uaccguggaa     1560

guccaauacg cuggcacgga cgggcccugc aaagugccug cccagauggc uguggacaug     1620

cagacccuga cacccguugg aagacugauc accgccaacc ccgucaucac agagucuacc     1680

gaaaacucca agaugaugcu cgagcuggau ccuccguucg gcgacagcua cauugugauc     1740

ggggugggcg agaaaaagau uacgcaccac uggcaucgcu ccggaucuac aaucggcaag     1800

gcguucgaag ccaccguccg gggcgcuaaa agaauggccg ugcuuggaga uacagcuugg     1860

gacuuuggca gcguuggggg agcccucaau ucccucggca aggggauuca ccaaaucuuc     1920

ggcgccgcuu uuaaaucucu guucggaggc augagcuggu uuucccagau ccugauuggg     1980

acccuucuca uguggcuggg acugaacgcg aagaauggca gcaucucucu caugugucug     2040

gcccuugggg gagugcugau uuuccucucc accgcuguca gcgcc                     2085


<210>  9883
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9883
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcgauuac cagacgggga agcgcuuacu auauguaccu ggaccgcucc      120

gaugccggca aggccaucuc uuucgcuaca acgcuugggg ugaacaaaug ccacgugcaa      180

auuauggacc ugggacauau gugugaugcc accaugagcu augaaugccc uaugcucgac      240

gagggcgucg aaccggauga cguggauugu uggugcaaua caaccuccac auggguugug      300

uacgggaccu gucaccauaa gaaaggagag gcuagacggu cucgcagagc cgucacacug      360

cccagccacu ccacccggaa gcugcagacg cgcagccaga ccuggcugga aucuagagag      420

uauacaaaac aucucaucaa gguggagaac uggaucuuuc ggaauccugg cuucgcgcug      480

guugccgugg cuauugccug gcugcucggc uccagcaccu cucagaaggu caucuaccug      540

gugaugauuc uucugaucgc ucccgccuau uccauucgcu gcaucggggu gagcaacaga      600

gacuuugucg aaggaauguc uggcgggaca uggguugaug uggugcucga gcacggaggc      660

ugugucaccg ugauggcgca agacaaaccu acgguugaua uugaacuggu gacaaccaca      720

gucuccaaua uggccgaggu gcggagcuac ugcuacgagg cuuccaucuc ugacauggcc      780

agcgauuccc gcugucccac ccagggggag gcuuaucuug acaagcagag cgacacccag      840

uacguuugca aaagaacacu gguggaucgg ggauggggca acggcugugg acucuucggg      900

aagggcucuc uggucacgug cgccaaauuu accugcucca agaagaugac agggaaaagc      960

auccaaccug aaaaucugga auaucgcauu augcucucug ugcacggaag ccagcauucc     1020

ggcaugaucg gguacgagac cgacgaagau agagccaagg ucgaggugac accgaacucu     1080

ccccgggcug aggcgacccu gggaggcuuc ggcagccuug ggcuggacug ugaaccuaga     1140

acgggacucg auuuuuccga ccuguauuac cuuacaauga auaacaaaca cuggcugguu     1200

cauaaggagu gguuccacga uauuccccuc ccuuggcacg ccggcgcuga caccgggaca     1260

ccccauugga auaacaaaga agcccuggug gaguuuaagg augcccacgc uaaacgccag     1320

accgucgugg uucugggaag ccaggaaggc gccgugcaua ccgcgcucgc uggggcccug     1380

gaggccgaga uggacggcgc uaagggacgg cuuuucucug gccaccugaa gugcagacuc     1440

aaaauggaua agcugcgccu uaaagggguc uccuauagcc uguguacagc cgcuuuuacg     1500

uucaccaagg ugccggccga aacacuccau ggaaccguga caguugaggu ccaauacgcg     1560

ggcaccgacg ggccuugcaa aauccccgug cagauggccg uggacaugca gacgcugacc     1620

ccugucggac ggcugauuac agcuaauccc gugaucaccg aaucuacaga gaacuccaag     1680

augaugcucg aacuggaucc cccuuucggc gacagcuaca ucguuauugg ggugggcgau     1740

aagaaaauca cccaccacug gcauagaucc ggaucuacga uuggcaaggc cuuugaggcu     1800

acaguccgcg gggccaaacg gauggcggug cuuggagaca ccgccuggga uuucggcagc     1860

guugggggag uguuuaauuc ccugggcaag gggauccacc aaauuuucgg cgcugccuuu     1920

aaaucucucu ucggaggcau gagcugguuu ucccagaucc ugaucgggac acugcuuguc     1980

uggcucggac ugaacaccaa gaacggcagc auuucucuga ccugucucgc ucuuggggga     2040

gugaugaucu uccuuuccac agccguuagc gcc                                  2073


<210>  9884
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9884
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggugac cagacgggga agcgcuuacu auauguaccu ggaccgcaac      120

gaugccggcg aagccauuuc cuucccuaca acgcuuggga ugaauaagug cuauauccaa      180

auuauggacc ugggacacau gugugaugcu accaugucuu acgagugccc gaugcucgac      240

gaaggcgugg agcccgauga cgucgauugu uggugcaaca caaccagcac augggugguu      300

uaugggaccu gucaucacaa aaagggagaa gccagacggu cccgcagagc ugugacacug      360

ccuucucaua gcacccggaa acuucagacg cgcucccaga ccuggcugga gagcagagag      420

uacacaaagc accucauccg ggucgaaaau uggaucuuuc gcaaccccgg cuucgcccug      480

gcggccgcug ccauugcuug gcugcucggg ucuuccacca gccagaaagu gaucuaucug      540

guuaugauuc uucugaucgc cccugcguac ucuauuagau gcaucggggu guccaaucgg      600

gacuuugucg agggaaugag cggcgggaca uggguggaug ugguccucga acauggaggc      660

uguguuaccg ugauggccca agacaagccc acgguggaua uugagcuggu cacaaccaca      720

gugucuaaca uggcugaggu ucgcuccuac ugcuaugaag ccagcaucuc cgacauggcu      780

ucugauagca gauguccuac ccagggggag gccuaccuug acaagcaguc cgacacccag      840

uaugugugca aacggacacu ggucgaucgc ggauggggca auggcugugg gcuguucgga      900

aagggcagcc uggugacgug cgccaaauuu gcuugcucua agaaaaugac cgggaagucc      960

auccaaccgg aaaaccugga guacagaauu augcucagcg uucacggauc ucagcacagc     1020

ggcaugaucg ugaaugacac agggcaugaa accgaugaga accgggcgaa ggucgagauu     1080

acacccaauu ccccuagagc cgaagcuacc cugggaggcu ucggcucucu ugggcuggac     1140

ugugagcccc gcacgggacu cgauuuuagc gaccuguauu accuuacaau gaacaauaaa     1200

cacuggcugg ugcauaagga augguuccac gauaucccuc uccccuggca cgccggcgcc     1260

gacaccggga ccccgcauug gaacaauaaa gaggcucugg ucgaauuuaa ggaugcccac     1320

gcgaaacggc agacaguggu ugugcuggga ucccaggagg gcgcugucca uaccgcccuc     1380

gccggggcuc uggaggccga aauggacggc gcuaagggaa gacuuagcuc uggccaccug     1440

aaaugccgcc ucaagaugga uaagcugcgg cuuaaagggg uguccuauag ccuguguaca     1500

gccgcguuca cguuuaccaa gauuccugcc gagacacucc auggaaccgu uacaguggaa     1560

guccaauacg cuggcaccga cgggcccugc aaagugccug cccagauggc uguggacaug     1620

cagacgcuga cccccguugg aagacugauc acagccaacc ccgucaucac cgagucuaca     1680

gaaaacucca agaugaugcu cgagcuggau ccuccguucg gcgacagcua cauugugauc     1740

ggggugggcg agaaaaagau uacccaccac uggcaucgcu ccggaucuac gaucggcaag     1800

gcguucgaag ccacaguccg gggcgcuaaa agaauggccg ugcuuggaga uaccgcuugg     1860

gacuuuggca gcguuggggg agcccucaau ucccucggca aggggauuca ccaaaucuuc     1920

ggcgccgcuu uuaaaucucu guucggaggc augagcuggu uuucccagau ccugauuggg     1980

acacuucuca uguggcuggg acugaacacc aagaauggca gcaucucucu caugugucug     2040

gcgcuugggg gagugcugau uuuccucucc accgccguca gcgcu                     2085


<210>  9885
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9885
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcguuacg       60

cggcgcggcu ccgcguacua uauguaccug gauagaaacg acgcugggga ggccauuucu      120

uuucccacca cacuuggaau gaauaagugc uauauccaga uuauggaucu gggccauaug      180

ugugacgcca ccaugagcua cgaaugcccu augcucgaug agggggugga accggacgau      240

guggacuguu ggugcaacac aaccuccaca ugggucgugu auggaaccug ucaccauaaa      300

aagggcgagg cucggcgcuc uagacgggcc guuacgcugc ccagccacuc cacccgcaaa      360

cuucaaacaa gaucucagac cuggcuggaa agccgggagu acacaaagca ucucauccgc      420

guggagaauu ggauuuucag aaacccuggg uuugcucugg ccgcggccgc uaucgccugg      480

cugcucggau ccagcaccuc ucagaaaguc aucuaucugg ugaugauucu ucugaucgcu      540

cccgccuacu ccauucggug caucggcguu agcaaucgcg auuucgugga aggcaugucu      600

gggggaacgu gggucgacau ugugcucgag cacggcgggu gugugacagu cauggcgcag      660

gauaagccua ccguugacau cgaacuggug acaaccaccg uguccaacau ggccgagguc      720

agaagcuacu gcuaugaggc uucuauuucc gauauggcca gcgacucccg gugucccaca      780

caaggagagg cuuaccuuga caagcagucu gauacgcagu augugugcaa acgcacccug      840

guugacagag gcugggggaa uggauguggc cucuuuggca aggggagccu ggugacaugc      900

gccaaauucg ccugcuccaa gaaaaugacc ggaaagagca uccagccuga aaaccuggaa      960

uaccggauca ugcucucugu ccacggcucc caacauagcg ggaugauugu gaaugauaca     1020

ggacacgaga ccgacgaaaa cagagcuaag guugagauca cgcccaauuc uccccgcgcg     1080

gaggccacac ugggcggguu uggaagccuu ggccuggauu gugaaccucg gaccggccuc     1140

gacuucuccg aucuguauua ccuuaccaug aacaauaaac auuggcuggu gcacaaggag     1200

ugguuucacg acauuccccu cccuuggcau gcuggggccg auacaggaac cccgcacugg     1260

aacaauaaag aagcccuggu cgaguucaag gacgcucaug ccaaaagaca gacagugguc     1320

gugcugggcu cucaggaagg ggcgguucac acggcucucg ccggagcccu ggaggcugag     1380

auggauggcg ccaaggggcg ccuuagcucc ggccaucuga aaugccggcu caagauggac     1440

aagcugagac uuaaaggagu gagcuauucu cuguguaccg cugccuuuac auucaccaag     1500

aucccggcgg aaacacucca cggcaccguc acgguggagg uucaguacgc cgggaccgac     1560

ggaccuugca aagugcccgc ucaaauggcc gucgauaugc agacacugac cccugugggc     1620

cgccugauua cagcuaaccc cgugaucacc gaauccacgg agaacagcaa gaugaugcuc     1680

gaacuggauc ccccuuuugg ggacucuuac aucguuauug gagucggcga gaaaaagauc     1740

acacaccauu ggcaccgguc cgggagcacc auuggcaagg ccuucgaggc gacagugaga     1800

ggagccaaac gcauggcugu gcuuggcgac accgccuggg auuucggguc cgucggaggc     1860

gcucucaauu cucuggggaa gggaauccau cagauuuuug gcgccgccuu caaaagccug     1920

uuugggggca uguccugguu cucucaaauc cugaucggaa cccuucucau guggcugggc     1980

cugaacacaa agaaugggag cauuucccuc augugucugg cucuuggagg cgugcugauc     2040

uuucucagca cggcgguuuc ugcc                                            2064


<210>  9886
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9886
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcguuacg       60

cggcgcggcu ccgcguacua uauguaccug gauagaaacg acgcugggga ggccauuucu      120

uuucccacca cacuuggaau gaauaagugc uauauccaga uuauggaucu gggccauacc      180

ugugacgcca caaugagcua cgaaugcccu augcucgaug agggggugga accggacgau      240

guggacuguu ggugcaacac cacauccacc ugggucgugu auggaacgug ucaccauaaa      300

aagggcgagg cucggcgcuc uagacgggcc guuacccugc ccagccacuc cacacgcaaa      360

cuucaaacca gaucucagac auggcuggaa agccgggagu acaccaagca ucucauccgc      420

guggagaauu ggauuuucag aaacccuggg uuugcucugg ccgcggccgc uaucgccugg      480

cugcucggau ccagcacguc ucagaaaguc aucuaucugg ugaugauucu ucugaucgcu      540

cccgccuacu ccauucggug caucggcguu agcaaucgcg auuucgugga aggcaugucu      600

gggggaacau gggucgacgu ggugcucgag cacggcgggu gugucaccgu uauggcgcag      660

gauaagccua caguggacau ugaacuggug accaccacag ucuccaacau ggccgaggug      720

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      780

caaggagagg cuuaccuuga caagcagucu gauacccagu auguuugcaa acgcacacug      840

guggacagag gcugggggaa uggauguggc cucuuuggca aggggagccu ggugaccugc      900

gccaaauucg ccugcuccaa gaaaaugaca ggaaagagca uucagccuga aaaccuggaa      960

uaccggauca ugcucucugu ccacggcucc caacauagcg ggaugaucgu uaaugauacc     1020

ggacacgaga cggacgaaaa cagagcuaag guggagauua cacccaauuc uccccgcgcg     1080

gaggccaccc ugggcggguu uggaagccuu ggccuggauu gugaaccucg gaccggccuc     1140

gacuucuccg aucuguauua ccuuacaaug aacaauaaac auuggcuggu ccacaaggag     1200

ugguuucacg acaucccccu cccuuggcau gcuggggccg auaccggaac accccacugg     1260

aacaauaaag aagcccuggu ggaguucaag gacgcucaug ccaaaagaca gacggucgug     1320

guucugggcu cucaggaagg ggcggugcac accgcucucg ccggagcccu ggaggcugag     1380

auggauggcg ccaaggggcg ccuuagcucc ggccaucuga aaugccggcu caagauggac     1440

aagcugagac uuaaaggagu cagcuauucu cuguguacag cugccuuuac cuucacaaag     1500

auuccggcgg aaacccucca cggcacggug accguugagg ugcaguacgc cgggacagac     1560

ggaccuugca aaguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1620

cgccugauca ccgcuaaccc cguuauuacg gaauccacag agaacagcaa gaugaugcuc     1680

gaacuggacc cgccuuuugg ggauucuuac aucgucaucg gagugggcga gaaaaagauu     1740

acccaccauu ggcaccgguc cgggagcaca aucggcaagg ccuucgaggc gaccgugaga     1800

ggagccaaac gcauggcugu ccuuggcgac accgccuggg auuucggguc cgugggaggc     1860

gcucucaauu cucuggggaa gggcauucau cagaucuuug gagccgccuu caaaagccug     1920

uuugggggca uguccugguu cucucaaauu cugaucggaa cacuucucau guggcugggc     1980

cugaacgcua agaaugggag caucucccuc augugucugg cgcuuggagg cguucugauu     2040

uuucucagca cggccguguc ugcu                                            2064


<210>  9887
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9887
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcauuacg       60

cggcgcggcu ccgcguacua uauguaccug gauagaucug acgcugggaa ggccaucagc      120

uuugccacca cacuuggagu uaacaaaugc caugugcaga uuauggaucu gggccacaug      180

ugugacgcua ccauguccua ugagugcccc augcucgaug aaggggugga gcccgacgau      240

gucgacuguu ggugcaauac aaccucuaca ugggugguuu acggaaccug ucaucacaag      300

aaaggcgaag cccggcgcag cagacgggcu gugacgcugc cgucccauuc uacccgcaaa      360

cuucaaacaa gaagccagac cuggcuggag ucccgggaau auacaaagca ccucaucaag      420

gucgagaacu ggauuuuccg caaucccggg uuugcccugg uggcgguugc caucgcuugg      480

cugcucggaa gcucuaccuc ccagaaggug aucuaccugg ucaugauucu ucugaucgcc      540

ccugcuuaua gcauuagaug caucggcgug ucuaaccggg auuucgugga gggcaugucc      600

gggggaacgu gggucgacgu ugugcucgaa cacggcgggu gugugacagu cauggcccag      660

gauaaaccca ccguggacau ugagcugguu acaaccaccg ugagcaauau ggcggaaguc      720

cgcucuuacu gcuacgaggc cuccaucagc gauauggcuu ccgacucuag auguccuaca      780

caaggagagg ccuaucuuga caagcagagc gauacgcagu acgugugcaa acggacccug      840

guugaccgcg gcugggggaa cggauguggc cucuuuggca aggggucccu ggugacaugc      900

gcuaaauuua ccugcagcaa gaagaugaca ggaaaaucua uucagcccga aaaucuggag      960

uauagaauca ugcucuccgu ccauggcagc caacacucug ggaugaucgg auacgaaacc     1020

gaugaggacc gggccaaggu ggaagucacg ccuaacagcc cgagagccga ggcuacacug     1080

ggcggguuug gaucccuugg ccuggauugu gagccccgca ccggccucga cuucucugau     1140

cuguauuacc uuaccaugaa uaacaaacau uggcuggugc acaaggaaug guuucacgac     1200

auuccucucc ccuggcaugc gggggccgau acaggaaccc cucacuggaa uaacaaagag     1260

gcucugguug aguucaagga cgcccaugcc aaacggcaga caguggucgu gcugggcagc     1320

caggaagggg cuguucacac ggcccucgcg ggagcucugg aagccgagau ggauggcgcc     1380

aaggggagac uuuucuccgg ccaucugaag ugccgccuca aaauggacaa gcugcggcuu     1440

aaaggaguga gcuauucucu guguaccgcu gccuucacau uuaccaaggu ccccgcugag     1500

acacuccacg gcaccgugac gguggaaguu caguacgccg ggaccgacgg accgugcaaa     1560

aucccugucc aaauggcggu ggauaugcag acacugaccc ccgugggcag acugauuaca     1620

gccaauccug ucaucaccga guccacggaa aacagcaaga ugaugcucga gcuggacccu     1680

cccuucgggg auucuuacau ugugaucgga guuggcgaca agaaaaucac acaccauugg     1740

caccgcuccg ggagcaccau uggcaaggcu uucgaagcca cagugcgggg agcuaaaaga     1800

auggccgucc uuggcgauac cgcgugggac uuuggguccg ugggaggcgu uuucaauucu     1860

cuggggaagg gaauccauca gauuuuuggc gccgcuuuca aaagccucuu ugggggcaug     1920

uccugguucu cucaaauccu gauuggaacc cugcuugugu ggcucggccu gaacacaaag     1980

aacgggagca ucucccugac gugucucgcc cugggaggcg ucaugaucuu ucuuagcacc     2040

gcugugucug cc                                                         2052


<210>  9888
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9888
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcguuacg       60

cggcgcggcu ccgcguacua uauguaccug gauagaaacg acgcugggga ggccauuucu      120

uuucccacca cacuuggaau gaauaagugc uauauccaga uuauggaucu gggccauaug      180

ugugacgcca ccaugagcua cgaaugcccu augcucgaug agggggugga accggacgau      240

guggacuguu ggugcaacac aaccuccaca ugggucgugu auggaaccug ucaccauaaa      300

aagggcgagg cucggcgcuc uagacgggcc guuacgcugc ccagccacuc cacccgcaaa      360

cuucaaacaa gaucucagac cuggcuggaa agccgggagu acacaaagca ucucauccgc      420

guggagaauu ggauuuucag aaacccuggg uuugcucugg ccgcggccgc uaucgccugg      480

cugcucggau ccagcaccuc ucagaaaguc aucuaucugg ugaugauucu ucugaucgcu      540

cccgccuacu ccauucggug caucggcguu agcaaucgcg auuucgugga aggcaugucu      600

gggggaacgu gggucgacgu ggugcucgag cacggcgggu gugucacagu uauggcgcag      660

gauaagccua ccguggacau ugaacuggug acaaccaccg ucuccaacau ggccgaggug      720

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccaca      780

caaggagagg cuuaccuuga caagcagucu gauacgcagu auguuugcaa acgcacccug      840

guggacagag gcugggggaa uggauguggc cucuuuggca aggggagccu ggucacaugc      900

gccaaauucg ccugcuccaa gaaaaugacc ggaaagagca uucagccuga aaaccuggaa      960

uaccggauca ugcucucugu gcacggcucc caacauagcg ggaugaucgu uaaugauaca     1020

ggacacgaga ccgacgaaaa cagagcuaag guggagauua cgcccaauuc uccccgcgcg     1080

gaggccacac ugggcggguu uggaagccuu ggccuggauu gugaaccucg gaccggccuc     1140

gacuucuccg aucuguauua ccuuaccaug aacaauaaac auuggcuggu ccacaaggag     1200

ugguuucacg acaucccccu cccuuggcau gcuggggccg auacaggaac cccgcacugg     1260

aacaauaaag aagcccuggu ggaguucaag gacgcucaug ccaaaagaca gacagucgug     1320

guucugggcu cucaggaagg ggcggugcac acggcucucg ccggagcccu ggaggcugag     1380

auggauggcg ccaaggggcg ccuuagcucc ggccaucuga aaugccggcu caagauggac     1440

aagcugagac uuaaaggagu cagcuauucu cuguguaccg cugccuuuac auucaccaag     1500

auuccggcgg aaacacucca cggcaccgug acgguugagg ugcaguacgc cgggaccgac     1560

ggaccuugca aaguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1620

cgccugauca cagcuaaccc cguuauuacc gaauccacgg agaacagcaa gaugaugcuc     1680

gaacuggauc ccccuuuugg ggacucuuac aucgucaucg gagugggcga gaaaaagauu     1740

acacaccauu ggcaccgguc cgggagcacc aucggcaagg ccuucgaggc gacagugaga     1800

ggagccaaac gcauggcugu ccuuggcgac accgccuggg auuucggguc cgugggaggc     1860

gcucucaauu cucuggggaa gggcauucau cagaucuuug gagccgccuu caaaagccug     1920

uuugggggca uguccugguu cucucaaauu cugaucggaa cccuucucau guggcugggc     1980

cugaacacaa agaaugggag caucucccuc augugucugg cucuuggagg cguucugauu     2040

uuucucagca cggcgguguc ugcc                                            2064


<210>  9889
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9889
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccguuacccg gcgcggcagc gccuacuaua uguaccugga cagaaacgau      120

gcuggggagg ccaucuccuu ccccacaacg cuuggaauga auaaauguua uauucagauc      180

auggaccugg gccacaugug cgaugcuacc augucuuacg aauguccuau gcucgacgag      240

gggguggaac cggaugacgu ggauugcugg uguaacacaa ccagcacaug ggucguguau      300

ggaaccugcc aucacaagaa aggcgaggcc cggcgcucca gacgggcggu uacacugccc      360

ucucauagca cccgcaaacu ucaaacgaga ucccagaccu ggcuggaaag ccgggaguac      420

acaaaacacc ucauucgcgu ggagaauugg aucuuuagaa acccuggcuu cgcccuggcu      480

gccgcugcca ucgcguggcu gcucgggucu uccaccagcc agaaggucau uuaucuggug      540

augauccuuc ugauugcccc cgcuuacucu auccggugua uuggaguuuc caaucgcgac      600

uuuguggaag gcaugagcgg gggaacaugg gucgauaucg ugcucgagca uggcgggugc      660

gugaccguca uggcccagga caagccuacg guugauauug aacuggugac aaccacagug      720

ucuaacaugg cugaggucag auccuacugu uaugaggcca gcaucuccga cauggccucu      780

gauagccggu gccccaccca aggagaggcu uaccuugaca aacaguccga cacccaguau      840

guguguaagc gcacacuggu ugauagaggc uggggcaaug ggugcggacu cuucggcaaa      900

gggagccugg ugacgugcgc gaaguuugcc uguucuaaaa agaugaccgg aaaguccauc      960

cagccugaaa accuggaaua ccggauuaug cucagcgucc acggcucuca acacagcggg     1020

augaucguga augacacagg acaugagacc gaugaaaaca gagcuaaagu ugagauuaca     1080

cccaauuccc cgcgcgccga ggccacccug ggcggcuucg ggucucuugg acuggacugc     1140

gaaccucgga cgggccucga uuuuagcgac cuguauuacc uuacaaugaa caauaagcac     1200

uggcuggugc auaaagagug guuccacgau aucccccucc cuuggcacgc uggggccgac     1260

accggaacac cccauuggaa caauaaggaa gcgcuggucg aguuuaaaga ugcucacgcc     1320

aagagacaga ccguggucgu gcugggcucc caggaagggg ccguucauac cgcucucgcc     1380

ggcgcucugg aggccgagau ggacggagcg aaaggccgcc uuagcucugg gcaccugaag     1440

ugucggcuca agauggauaa acugagacuu aagggagugu ccuauagccu gugcacagcc     1500

gcuuucacgu uuaccaaaau uccggccgaa acacuccaug gcaccgucac aguggagguu     1560

caguacgcug ggaccgacgg accuuguaag gugcccgccc aaauggcggu cgacaugcag     1620

acgcugaccc cugugggccg ccugaucaca gccaaccccg ugaucaccga aucuacagag     1680

aacuccaaaa ugaugcucga acuggauccc ccuuucgggg acagcuacau uguuaucggc     1740

gucggagaga agaagauuac ccaccacugg caucgguccg gcucuacgau cgggaaggcu     1800

uuugaggcca cagugagagg agcuaagcgc auggccgugc uuggcgauac cgccugggac     1860

uucgggagcg ucggaggcgc ucucaauucc cuggggaaag gcauucacca gaucuucgga     1920

gcggccuuua agucucuguu cggcgggaug agcugguuuu cccaaauccu gauuggaaca     1980

cuucucaugu ggcugggccu gaacaccaaa aaugggagca ucucucucau gugccuggcu     2040

cuuggaggcg ugcugauuuu ccucuccacc gccguuagcg cc                        2082


<210>  9890
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9890
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccguuacccg gcgcggcagc gccuacuaua uguaccugga cagaaacgau      120

gcuggggagg ccaucuccuu ccccacaacg cuuggaauga auaaauguua uauucagauc      180

auggaccugg gccauaccug cgaugcuaca augucuuacg aauguccuau gcucgacgag      240

gggguggaac cggaugacgu ggauugcugg uguaacacca caagcaccug ggucguguau      300

ggaacaugcc accacaagaa aggcgaggcc cggcgcucca gacgggcggu uacccugccc      360

ucucauagca cgcgcaaacu ucaaaccaga ucccagacau ggcuggaaag ccgggaguac      420

accaaacacc ucauucgcgu ggagaauugg aucuuuagaa acccuggcuu cgcccuggcu      480

gccgcugcca ucgcguggcu gcucgggucu uccacaagcc agaaggucau uuaucuggug      540

augauccuuc ugauugcccc cgcuuacucu auccggugua uuggaguuuc caaucgcgac      600

uuuguggaag gcaugagcgg gggaaccugg gucgaugugg ugcucgagca uggcgggugc      660

gucacgguua uggcccagga caagccuaca guggauaucg aacuggugac cacaaccguc      720

ucuaacaugg cugaggugag auccuacugu uaugaggcca gcauuuccga cauggccucu      780

gauagccggu gccccaccca aggagaggcu uaccuugaca aacaguccga cacacaguau      840

guuuguaagc gcacgcuggu ggauagaggc uggggcaaug ggugcggacu cuucggcaaa      900

gggagccugg ugaccugcgc gaaguuugcc uguucuaaaa agaugacagg aaaguccauc      960

cagccugaaa accuggaaua ccggaucaug cucagcgucc acggcucuca acacagcggg     1020

augauuguua augacaccgg acaugagaca gaugaaaaca gagcuaaagu ggagaucacc     1080

cccaauuccc cgcgcgccga ggccacgcug ggcggcuucg ggucucuugg acuggacugc     1140

gaaccucgga caggccucga uuuuagcgac cuguauuacc uuaccaugaa caauaagcac     1200

uggcuggucc auaaagagug guuccacgau auuccccucc cuuggcacgc uggggccgac     1260

accggaacac cccauuggaa caauaaggaa gcgcuggugg aguuuaaaga ugcucacgcc     1320

aagagacaga ccgucguggu ucugggcucc caggaagggg ccgugcauac agcucucgcc     1380

ggcgcucugg aggccgagau ggacggagcg aaaggccgcc uuagcucugg gcaccugaag     1440

ugucggcuca agauggauaa acugagacuu aagggagucu ccuauagccu gugcacggcc     1500

gcuuucaccu uuacaaaaau cccggccgaa acccuccaug gcacagugac cguugaggug     1560

caguacgcug ggacggacgg accuuguaag guccccgccc aaauggcggu ggacaugcag     1620

acccugacac cugugggccg ccugauuacc gccaaccccg uuaucacaga aucuaccgag     1680

aacuccaaaa ugaugcucga acuggauccc ccuuucgggg acagcuacau cgucauuggc     1740

gugggagaga agaagaucac gcaccacugg caucgguccg gcucuacaau ugggaaggcu     1800

uuugaggcca ccgugagagg agcuaagcgc auggccgucc uuggcgauac agccugggac     1860

uucgggagcg ugggaggcgc ucucaauucc cuggggaaag gcauccacca gauuuucgga     1920

gcggccuuua agucucuguu cggcgggaug agcugguuuu cccaaauccu gaucggaacc     1980

cuucucaugu ggcugggccu gaacgcuaaa aaugggagca uuucucucau gugccuggcc     2040

cuuggaggcg uucugaucuu ccucuccacc gccgugagcg cu                        2082


<210>  9891
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9891
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccaucacccg gcgcggcagc gccuacuaua uguaccugga cagauccgau      120

gcugggaaag ccauuucuuu cgcuacaacg cuuggaguua acaaguguca cgugcagauc      180

auggaccugg gccauaugug cgaugccacc augagcuaug agugucccau gcucgacgaa      240

gggguggagc ccgaugacgu cgauugcugg uguaauacaa ccuccacaug ggugguuuac      300

ggaaccugcc accauaaaaa gggcgaagcg cggcgcucua gacgggccgu gacacugccg      360

agccacucca cccgcaaacu ucaaacgaga agccagaccu ggcuggaguc ucgggaauau      420

acaaagcauc ucauuaaggu cgagaacugg aucuuucgca aucccggcuu cgcucuggug      480

gccguugcua ucgccuggcu gcucgggucc agcaccucuc agaaagugau uuaccugguc      540

augauccuuc ugauugcgcc ugccuauucc aucagaugua uuggagugag caaccgggac      600

uuuguggagg gcaugucugg gggaacaugg gucgauguug ugcucgaaca cggcgggugc      660

gugaccguca uggcucagga caagcccacg guggauaucg agcugguuac aaccacagug      720

uccaauaugg ccgaaguccg cagcuacugu uacgaggcuu ccauuucuga cauggccagc      780

gauuccagau gcccuaccca aggagaggcc uaucuugaca aacagagcga cacccaguac      840

guguguaagc ggacacuggu ugaucgcggc uggggcaacg ggugcggacu cuucggcaaa      900

gggucucugg ugacgugcgc uaaguuuacc uguuccaaga aaaugacagg aaagagcauc      960

cagcccgaaa aucuggagua uagaaucaug cucucugucc acggcagcca acauuccggg     1020

augauuggau acgaaaccga cgaggaucgg gcgaaagugg aagucacacc uaacucuccg     1080

agagccgagg cuacccuggg cggcuucggg agccuuggac uggacugcga gccccgcacg     1140

ggccucgauu uuuccgaccu guauuaccuu acaaugaaua acaagcacug gcuggugcau     1200

aaagaauggu uccacgauau cccucucccc uggcacgccg gggccgacac cggaaccccu     1260

cauuggaaua acaaggaggc ucugguugaa uuuaaagaug cccacgcgaa gcggcagaca     1320

guggucgugc ugggcagcca ggagggggcu guucauaccg cccucgccgg cgcucuggaa     1380

gccgagaugg acggagcuaa gggcagacuu uucucugggc accugaaaug ucgccucaag     1440

auggauaaac ugcggcuuaa gggagugucc uauagccugu gcacagccgc guuuacguuc     1500

accaaagucc ccgccgagac acuccauggc accgugacag uggaaguuca guacgcuggg     1560

accgacggac cguguaagau uccuguccaa auggccgugg acaugcagac gcugaccccc     1620

gugggcagac ugaucacagc uaauccuguc auuaccgagu cuacagaaaa cuccaagaug     1680

augcucgagc uggauccucc cuuuggggac agcuacaucg ugaucggcgu uggagauaaa     1740

aagauuaccc accacuggca ucgcuccggc ucuacgaucg ggaaagccuu cgaagcgaca     1800

gugcggggag ccaagagaau ggcuguccuu ggcgacaccg ccugggauuu cgggagcgug     1860

ggaggcguuu uuaauucccu ggggaaaggc auucaccaga ucuucggagc ugccuuuaag     1920

ucucucuucg gcgggaugag cugguuuucc caaauucuga ucggaacacu gcuugugugg     1980

cucggccuga acaccaagaa cgggagcauc ucucugaccu gccucgcccu gggaggcguc     2040

augauuuucc uuuccacagc ugugagcgcg                                      2070


<210>  9892
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9892
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccguuacccg gcgcggcagc gccuacuaua uguaccugga cagaaacgau      120

gcuggggagg ccaucuccuu ccccacaacg cuuggaauga auaaauguua uauucagauc      180

auggaccugg gccacaugug cgaugcuacc augucuuacg aauguccuau gcucgacgag      240

gggguggaac cggaugacgu ggauugcugg uguaacacaa ccagcacaug ggucguguau      300

ggaaccugcc aucacaagaa aggcgaggcc cggcgcucca gacgggcggu uacacugccc      360

ucucauagca cccgcaaacu ucaaacgaga ucccagaccu ggcuggaaag ccgggaguac      420

acaaaacacc ucauucgcgu ggagaauugg aucuuuagaa acccuggcuu cgcccuggcu      480

gccgcugcca ucgcguggcu gcucgggucu uccaccagcc agaaggucau uuaucuggug      540

augauccuuc ugauugcccc cgcuuacucu auccggugua uuggaguuuc caaucgcgac      600

uuuguggaag gcaugagcgg gggaacaugg gucgaugugg ugcucgagca uggcgggugc      660

gucaccguua uggcccagga caagccuacg guggauaucg aacuggugac aaccacaguc      720

ucuaacaugg cugaggugag auccuacugu uaugaggcca gcauuuccga cauggccucu      780

gauagccggu gccccaccca aggagaggcu uaccuugaca aacaguccga cacccaguau      840

guuuguaagc gcacacuggu ggauagaggc uggggcaaug ggugcggacu cuucggcaaa      900

gggagccugg ucacgugcgc gaaguuugcc uguucuaaaa agaugaccgg aaaguccauc      960

cagccugaaa accuggaaua ccggaucaug cucagcgugc acggcucuca acacagcggg     1020

augauuguua augacacagg acaugagacc gaugaaaaca gagcuaaagu ggagaucaca     1080

cccaauuccc cgcgcgccga ggccacccug ggcggcuucg ggucucuugg acuggacugc     1140

gaaccucgga cgggccucga uuuuagcgac cuguauuacc uuacaaugaa caauaagcac     1200

uggcuggucc auaaagagug guuccacgau auuccccucc cuuggcacgc uggggccgac     1260

accggaacac cccauuggaa caauaaggaa gcgcuggugg aguuuaaaga ugcucacgcc     1320

aagagacaga ccgucguggu ucugggcucc caggaagggg ccgugcauac cgcucucgcc     1380

ggcgcucugg aggccgagau ggacggagcg aaaggccgcc uuagcucugg gcaccugaag     1440

ugucggcuca agauggauaa acugagacuu aagggagucu ccuauagccu gugcacagcc     1500

gcuuucacgu uuaccaaaau cccggccgaa acacuccaug gcaccgugac aguugaggug     1560

caguacgcug ggaccgacgg accuuguaag guccccgccc aaauggcggu ggacaugcag     1620

acgcugaccc cugugggccg ccugauuaca gccaaccccg uuaucaccga aucuacagag     1680

aacuccaaaa ugaugcucga acuggauccc ccuuucgggg acagcuacau cgucauuggc     1740

gugggagaga agaagaucac ccaccacugg caucgguccg gcucuacgau ugggaaggcu     1800

uuugaggcca cagugagagg agcuaagcgc auggccgucc uuggcgauac cgccugggac     1860

uucgggagcg ugggaggcgc ucucaauucc cuggggaaag gcauccacca gauuuucgga     1920

gcggccuuua agucucuguu cggcgggaug agcugguuuu cccaaauccu gaucggaaca     1980

cuucucaugu ggcugggccu gaacaccaaa aaugggagca uuucucucau gugccuggcu     2040

cuuggaggcg uucugaucuu ccucuccacc gccgugagcg cc                        2082


<210>  9893
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9893
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggugac cagacgggga agcgcuuacu auauguaccu ggaccgcaac      120

gaugccggcg aagccauuuc cuucccuaca acgcuuggga ugaauaagug cuauauccaa      180

auuauggacc ugggacacau gugugaugcu accaugucuu acgagugccc gaugcucgac      240

gaaggcgugg agcccgauga cgucgauugu uggugcaaca caaccagcac augggugguu      300

uaugggaccu gucaucacaa aaagggagaa gccagacggu cccgcagagc ugugacacug      360

ccuucucaua gcacccggaa acuucagacg cgcucccaga ccuggcugga gagcagagag      420

uacacaaagc accucauccg ggucgaaaau uggaucuuuc gcaaccccgg cuucgcccug      480

gcggccgcug ccauugcuug gcugcucggg ucuuccacca gccagaaagu gaucuaucug      540

guuaugauuc uucugaucgc cccugcguac ucuauuagau gcaucggggu guccaaucgg      600

gacuuugucg agggaaugag cggcgggaca uggguggaua uugugcucga acauggaggc      660

ugugucaccg uuauggccca agacaagccc acgguggaua ucgagcuggu gacaaccaca      720

gucucuaaca uggcugaggu gcgcuccuac ugcuaugaag ccagcaucuc cgacauggcu      780

ucugauagca gauguccuac ccagggggag gccuaccuug acaagcaguc cgacacccag      840

uauguuugca aacggacacu gguggaucgc ggauggggca auggcugugg ccucuucgga      900

aagggcagcc uggucacgug cgccaaauuu gcuugcucua agaaaaugac cgggaagucc      960

auucaaccgg aaaaccugga guacagaauc augcucagcg ugcacggauc ucagcacagc     1020

ggcaugauug uuaaugacac agggcaugaa accgaugaga accgggcgaa gguggagauc     1080

acacccaauu ccccuagagc cgaagcuacc cugggaggcu ucggcucucu ugggcuggac     1140

ugugagcccc gcacgggacu cgauuuuagc gaccuguauu accuuacaau gaacaauaaa     1200

cacuggcugg uccauaagga augguuccac gauauuccuc uccccuggca cgccggcgcc     1260

gacaccggga ccccgcauug gaacaauaaa gaggcucugg uggaauuuaa ggaugcccac     1320

gcgaaacggc agacagucgu gguucuggga ucccaggagg gcgcugugca uaccgcccuc     1380

gccggggcuc uggaggccga aauggacggc gcuaagggaa gacuuagcuc uggccaccug     1440

aaaugccgcc ucaagaugga uaagcugcgg cuuaaagggg ucuccuauag ccuguguaca     1500

gccgcguuca cguuuaccaa gaucccugcc gagacacucc auggaaccgu gacaguugaa     1560

gugcaauacg cuggcaccga cgggcccugc aaagucccug cccagauggc uguggacaug     1620

cagacgcuga cccccguggg aagacugauc acagccaacc ccguuauuac cgagucuaca     1680

gaaaacucca agaugaugcu cgagcuggau ccuccguucg gcgacagcua caucgucauu     1740

ggggugggcg agaaaaagau cacccaccac uggcaucgcu ccggaucuac gcuuggcaag     1800

gcguucagca caacccugaa aggggcccaa cggcucgcug cccugggaga uacagcuugg     1860

gacuuuggcu ccauuggggg aguguucaau ucuaucggca gagccgucca ccagguguuu     1920

gggggcgccu uccgcacccu guuuggaggc augagcugga ucacccaggg gcuuauggga     1980

gcucuccugc uguggauggg cguuaacgcg cgggauagau ccauugcccu cgcuuuccug     2040

gccacagggg gagugcuugu cuuucuggcc acgaaugugc augcu                     2085


<210>  9894
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9894
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggugac cagacgggga agcgcuuacu auauguaccu ggaccgcaac      120

gaugccggcg aagccauuuc cuucccuaca acgcuuggga ugaauaagug cuauauccaa      180

auuauggacc ugggacacac cugugaugcu acaaugucuu acgagugccc gaugcucgac      240

gaaggcgugg agcccgauga cgucgauugu uggugcaaca ccacaagcac cugggugguu      300

uaugggacau gucaucacaa aaagggagaa gccagacggu cccgcagagc ugugacccug      360

ccuucucaua gcacgcggaa acuucagacc cgcucccaga cauggcugga gagcagagag      420

uacaccaagc accucauccg ggucgaaaau uggaucuuuc gcaaccccgg cuucgcccug      480

gcggccgcug ccauugcuug gcugcucggg ucuuccacaa gccagaaagu gaucuaucug      540

guuaugauuc uucugaucgc cccugcguac ucuauuagau gcaucggggu guccaaucgg      600

gacuuugucg agggaaugag cggcgggacc uggguggaug ugguccucga acauggaggc      660

uguguuacgg ugauggccca agacaagccc acaguggaua uugagcuggu caccacaacc      720

gugucuaaca uggcugaggu ucgcuccuac ugcuaugaag ccagcaucuc cgacauggcu      780

ucugauagca gauguccuac ccagggggag gccuaccuug acaagcaguc cgacacacag      840

uaugugugca aacggacgcu ggucgaucgc ggauggggca auggcugugg ccucuucgga      900

aagggcagcc uggugaccug cgccaaauuu gcuugcucua agaaaaugac agggaagucc      960

auccaaccgg aaaaccugga guacagaauu augcucagcg uucacggauc ucagcacagc     1020

ggcaugaucg ugaaugacac cgggcaugaa acagaugaga accgggcgaa ggucgagauu     1080

acccccaauu ccccuagagc cgaagcuacg cugggaggcu ucggcucucu ugggcuggac     1140

ugugagcccc gcacaggacu cgauuuuagc gaccuguauu accuuaccau gaacaauaaa     1200

cacuggcugg ugcauaagga augguuccac gauaucccuc uccccuggca cgccggcgcc     1260

gacaccggga caccgcauug gaacaauaaa gaggcucugg ucgaauuuaa ggaugcccac     1320

gcgaaacggc agaccguggu ugugcuggga ucccaggagg gcgcugucca uacagcccuc     1380

gccggggcuc uggaggccga aauggacggc gcuaagggaa gacuuagcuc uggccaccug     1440

aaaugccgcc ucaagaugga uaagcugcgg cuuaaagggg uguccuauag ccuguguacg     1500

gccgcguuca ccuuuacaaa gauuccugcc gagacccucc auggaacagu uaccguggaa     1560

guccaauacg cuggcacgga cgggcccugc aaagugccug cccagauggc uguggacaug     1620

cagacccuga cacccguugg aagacugauc accgccaacc ccgucaucac agagucuacc     1680

gaaaacucca agaugaugcu cgagcuggau ccuccguucg gcgacagcua cauugugauc     1740

ggggugggcg agaaaaagau uacgcaccac uggcaucgcu ccggaucuac acuuggcaag     1800

gcguucagca ccacacugaa aggggcccaa cggcucgcug cccugggaga uaccgcuugg     1860

gacuuuggcu ccaucggggg agucuucaau ucuauuggca gagccgugca ccagguuuuu     1920

gggggcgccu uccgcacccu guuuggaggc augagcugga ucacacaggg gcuuauggga     1980

gcucuccugc uguggauggg cgugaacgcg cgggauagau ccaucgcccu cgcuuuccug     2040

gccaccgggg gaguccuugu guuucuggcc acgaauguuc augcu                     2085


<210>  9895
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9895
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcgauuac cagacgggga agcgcuuacu auauguaccu ggaccgcucc      120

gaugccggca aggccaucuc uuucgcuaca acgcuugggg ugaacaaaug ccacgugcaa      180

auuauggacc ugggacauau gugugaugcc accaugagcu augaaugccc uaugcucgac      240

gagggcgucg aaccggauga cguggauugu uggugcaaua caaccuccac auggguugug      300

uacgggaccu gucaccauaa gaaaggagag gcuagacggu cucgcagagc cgucacacug      360

cccagccacu ccacccggaa gcuccagacg cgcagccaga ccuggcugga aucuagagag      420

uauacaaaac aucucaucaa gguggagaac uggaucuuuc ggaauccugg cuucgcgcug      480

guugccgugg cuauugccug gcugcucggc uccagcaccu cucagaaggu caucuaccug      540

gugaugauuc uucugaucgc ucccgccuau uccauucgcu gcaucggggu gagcaacaga      600

gacuuugucg aaggaauguc uggcgggaca uggguugaug uggugcucga gcacggaggc      660

ugugucaccg ugauggcgca agacaaaccu acgguugaua uugaacuggu gacaaccaca      720

gucuccaaua uggccgaggu gcggagcuac ugcuacgagg cuuccaucuc ugacauggcc      780

agcgauuccc gcugucccac ccagggggag gcuuaucuug acaagcagag cgacacccag      840

uacguuugca aaagaacacu gguggaucgg ggauggggca acggcugugg gcuuuucgga      900

aagggcucuc uggucacgug cgccaaauuu accugcucca agaagaugac agggaaaagc      960

auccaaccug aaaaucugga auaucgcauu augcucucug ugcacggaag ccagcauucc     1020

ggcaugaucg gguacgagac cgacgaagau agagccaagg ucgaggugac accgaacucu     1080

ccccgggcug aggcgacccu gggaggcuuc ggcagccuug ggcuggacug ugaaccuaga     1140

acgggacucg auuuuuccga ccuguauuac cuuacaauga auaacaaaca cuggcugguu     1200

cauaaggagu gguuccacga uauuccccuc ccuuggcacg ccggcgcuga caccgggaca     1260

ccccauugga auaacaaaga agcccuggug gaguuuaagg augcccacgc uaaacgccag     1320

accgucgugg uucugggaag ccaggaaggc gccgugcaua ccgcgcucgc uggggcccug     1380

gaggccgaga uggacggcgc uaagggacgg cuuuucucug gccaccugaa gugcagacuc     1440

aaaauggaua agcugcgccu uaaagggguc uccuauagcc uguguacagc cgcuuuuacg     1500

uucaccaagg ugccggccga aacacuccau ggaaccguga caguugaggu ccaauacgcg     1560

ggcaccgacg ggccuugcaa aauccccgug cagauggccg uggacaugca gacgcugacc     1620

ccugucggac ggcugauuac agcuaauccc gugaucaccg aaucuacaga gaacuccaag     1680

augaugcucg aacuggaucc cccuuucggc gacagcuaca ucguuauugg ggugggcgau     1740

aagaaaauca cccaccacug gcauagaucc ggaucuacgc uuggcaaggc cuuuagcaca     1800

acccugaaag gggcucaacg ccucgccgcg cugggagaca cagccuggga uuucggcucc     1860

auugggggag ucuuuaauuc uaucggccgg gcugugcacc agguuuucgg gggcgccuuu     1920

agaacccugu ucggaggcau gagcuggauu acccaggggc uuaugggagc ucuccugcug     1980

uggaugggcg ugaacgcccg cgaccggucc aucgcccucg cuuuucuggc gacaggggga     2040

guccuugugu uccuggccac gaacguucau gcu                                  2073


<210>  9896
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9896
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggugac cagacgggga agcgcuuacu auauguaccu ggaccgcaac      120

gaugccggcg aagccauuuc cuucccuaca acgcuuggga ugaauaagug cuauauccaa      180

auuauggacc ugggacacau gugugaugcu accaugucuu acgagugccc gaugcucgac      240

gaaggcgugg agcccgauga cgucgauugu uggugcaaca caaccagcac augggugguu      300

uaugggaccu gucaucacaa aaagggagaa gccagacggu cccgcagagc ugugacacug      360

ccuucucaua gcacccggaa acuucagacg cgcucccaga ccuggcugga gagcagagag      420

uacacaaagc accucauccg ggucgaaaau uggaucuuuc gcaaccccgg cuucgcccug      480

gcggccgcug ccauugcuug gcugcucggg ucuuccacca gccagaaagu gaucuaucug      540

guuaugauuc uucugaucgc cccugcguac ucuauuagau gcaucggggu guccaaucgg      600

gacuuugucg agggaaugag cggcgggaca uggguggaug ugguccucga acauggaggc      660

uguguuaccg ugauggccca agacaagccc acgguggaua uugagcuggu cacaaccaca      720

gugucuaaca uggcugaggu ucgcuccuac ugcuaugaag ccagcaucuc cgacauggcu      780

ucugauagca gauguccuac ccagggggag gccuaccuug acaagcaguc cgacacccag      840

uaugugugca aacggacacu ggucgaucgc ggauggggca auggcugugg ccucuucgga      900

aagggcagcc uggugacgug cgccaaauuu gcuugcucua agaaaaugac cgggaagucc      960

auccaaccgg aaaaccugga guacagaauu augcucagcg uucacggauc ucagcacagc     1020

ggcaugaucg ugaaugacac agggcaugaa accgaugaga accgggcgaa ggucgagauu     1080

acacccaauu ccccuagagc cgaagcuacc cugggaggcu ucggcucucu ugggcuggac     1140

ugugagcccc gcacgggacu cgauuuuagc gaccuguauu accuuacaau gaacaauaaa     1200

cacuggcugg ugcauaagga augguuccac gauaucccuc uccccuggca cgccggcgcc     1260

gacaccggga ccccgcauug gaacaauaaa gaggcucugg ucgaauuuaa ggaugcccac     1320

gcgaaacggc agacaguggu ugugcuggga ucccaggagg gcgcugucca uaccgcccuc     1380

gccggggcuc uggaggccga aauggacggc gcuaagggaa gacuuagcuc uggccaccug     1440

aaaugccgcc ucaagaugga uaagcugcgg cuuaaagggg uguccuauag ccuguguaca     1500

gccgcguuca cguuuaccaa gauuccugcc gagacacucc auggaaccgu uacaguggaa     1560

guccaauacg cuggcaccga cgggcccugc aaagugccug cccagauggc uguggacaug     1620

cagacgcuga cccccguugg aagacugauc acagccaacc ccgucaucac cgagucuaca     1680

gaaaacucca agaugaugcu cgagcuggau ccuccguucg gcgacagcua cauugugauc     1740

ggggugggcg agaaaaagau uacccaccac uggcaucgcu ccggaucuac gcuuggcaag     1800

gcguucagca caacccugaa aggggcccaa cggcucgcug cccugggaga uacagcuugg     1860

gacuuuggcu ccaucggggg agucuucaau ucuauuggca gagccgugca ccagguuuuu     1920

gggggcgccu uccgcacccu guuuggaggc augagcugga ucacccaggg gcuuauggga     1980

gcucuccugc uguggauggg cgugaacgcg cgggauagau ccaucgcccu cgcuuuccug     2040

gccacagggg gaguccuugu guuucuggcc acgaauguuc augcu                     2085


<210>  9897
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9897
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcguuacg       60

cggcgcggcu ccgcguacua uauguaccug gauagaaacg acgcugggga ggccauuucu      120

uuucccacca cacuuggaau gaauaagugc uauauccaga uuauggaucu gggccauaug      180

ugugacgcca ccaugagcua cgaaugcccu augcucgaug agggggugga accggacgau      240

guggacuguu ggugcaacac aaccuccaca ugggucgugu auggaaccug ucaccauaaa      300

aagggcgagg cucggcgcuc uagacgggcc guuacgcugc ccagccacuc cacccgcaaa      360

cuucaaacaa gaucucagac cuggcuggaa agccgggagu acacaaagca ucucauccgc      420

guggagaauu ggauuuucag aaacccuggg uuugcucugg ccgcggccgc uaucgccugg      480

cugcucggau ccagcaccuc ucagaaaguc aucuaucugg ugaugauucu ucugaucgcu      540

cccgccuacu ccauucggug caucggcguu agcaaucgcg auuucgugga aggcaugucu      600

gggggaacgu gggucgacau ugugcucgag cacggcgggu gugugacagu cauggcgcag      660

gauaagccua ccguugacau cgaacuggug acaaccaccg uguccaacau ggccgagguc      720

agaagcuacu gcuaugaggc uucuauuucc gauauggcca gcgacucccg gugucccaca      780

caaggagagg cuuaccuuga caagcagucu gauacgcagu augugugcaa acgcacccug      840

guugacagag gcugggggaa uggauguggc cucuuuggca aggggagccu ggugacaugc      900

gccaaauucg ccugcuccaa gaaaaugacc ggaaagagca uccagccuga aaaccuggaa      960

uaccggauca ugcucucugu ccacggcucc caacauagcg ggaugauugu gaaugauaca     1020

ggacacgaga ccgacgaaaa cagagcuaag guugagauca cgcccaauuc uccccgcgcg     1080

gaggccacac ugggcggguu uggaagccuu ggccuggauu gugaaccucg gaccggccuc     1140

gacuucuccg aucuguauua ccuuaccaug aacaauaaac auuggcuggu gcacaaggag     1200

ugguuucacg acauuccccu cccuuggcau gcuggggccg auacaggaac cccgcacugg     1260

aacaauaaag aagcccuggu cgaguucaag gacgcucaug ccaaaagaca gacagugguc     1320

gugcugggcu cucaggaagg ggcgguucac acggcucucg ccggagcccu ggaggcugag     1380

auggauggcg ccaaggggcg ccuuagcucc ggccaucuga aaugccggcu caagauggac     1440

aagcugagac uuaaaggagu gagcuauucu cuguguaccg cugccuuuac auucaccaag     1500

aucccggcgg aaacacucca cggcaccguc acgguggagg uucaguacgc cgggaccgac     1560

ggaccuugca aagugcccgc ucaaauggcc gucgauaugc agacacugac cccugugggc     1620

cgccugauua cagcuaaccc cgugaucacc gaauccacgg agaacagcaa gaugaugcuc     1680

gaacuggauc ccccuuuugg ggacucuuac aucguuauug gagucggcga gaaaaagauc     1740

acacaccauu ggcaccgguc cgggagcacc cuuggcaagg ccuucuccac aacccugaaa     1800

ggagcgcaga gacucgccgc ucugggcgac accgccuggg auuucggguc uauuggaggc     1860

guguuuaaua gcaucgggcg cgcugugcau caagucuucg gaggcgccuu ucggacacug     1920

uucgggggca uguccuggau uacgcaggga cuuaugggcg cccuccugcu guggaugggg     1980

gugaacgcua gagaccgcuc uaucgcgcuc gccuuucugg cuaccggagg cguucuugug     2040

uuccuggcca caaaugucca cgcc                                            2064


<210>  9898
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9898
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcguuacg       60

cggcgcggcu ccgcguacua uauguaccug gauagaaacg acgcugggga ggccauuucu      120

uuucccacca cacuuggaau gaauaagugc uauauccaga uuauggaucu gggccauacc      180

ugugacgcca caaugagcua cgaaugcccu augcucgaug agggggugga accggacgau      240

guggacuguu ggugcaacac cacauccacc ugggucgugu auggaacgug ucaccauaaa      300

aagggcgagg cucggcgcuc uagacgggcc guuacccugc ccagccacuc cacacgcaaa      360

cuucaaacca gaucucagac auggcuggaa agccgggagu acaccaagca ucucauccgc      420

guggagaauu ggauuuucag aaacccuggg uuugcucugg ccgcggccgc uaucgccugg      480

cugcucggau ccagcacguc ucagaaaguc aucuaucugg ugaugauucu ucugaucgcu      540

cccgccuacu ccauucggug caucggcguu agcaaucgcg auuucgugga aggcaugucu      600

gggggaacau gggucgacgu ggugcucgag cacggcgggu gugucaccgu uauggcgcag      660

gauaagccua caguggacau ugaacuggug accaccacag ucuccaacau ggccgaggug      720

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccacg      780

caaggagagg cuuaccuuga caagcagucu gauacccagu auguuugcaa acgcacacug      840

guggacagag gcugggggaa uggauguggc cucuuuggca aggggagccu ggugaccugc      900

gccaaauucg ccugcuccaa gaaaaugaca ggaaagagca uucagccuga aaaccuggaa      960

uaccggauca ugcucucugu ccacggcucc caacauagcg ggaugaucgu uaaugauacc     1020

ggacacgaga cggacgaaaa cagagcuaag guggagauua cacccaauuc uccccgcgcg     1080

gaggccaccc ugggcggguu uggaagccuu ggccuggauu gugaaccucg gaccggccuc     1140

gacuucuccg aucuguauua ccuuacaaug aacaauaaac auuggcuggu ccacaaggag     1200

ugguuucacg acaucccccu cccuuggcau gcuggggccg auaccggaac accccacugg     1260

aacaauaaag aagcccuggu ggaguucaag gacgcucaug ccaaaagaca gacggucgug     1320

guucugggcu cucaggaagg ggcggugcac accgcucucg ccggagcccu ggaggcugag     1380

auggauggcg ccaaggggcg ccuuagcucc ggccaucuga aaugccggcu caagauggac     1440

aagcugagac uuaaaggagu cagcuauucu cuguguacag cugccuuuac cuucacaaag     1500

auuccggcgg aaacccucca cggcacggug accguugagg ugcaguacgc cgggacagac     1560

ggaccuugca aaguccccgc ucaaauggcc guggauaugc agacccugac accugugggc     1620

cgccugauca ccgcuaaccc cguuauuacg gaauccacag agaacagcaa gaugaugcuc     1680

gaacuggacc cgccuuuugg ggauucuuac aucgucaucg gagugggcga gaaaaagauu     1740

acccaccauu ggcaccgguc cgggagcaca cuuggcaagg ccuucuccac cacccugaaa     1800

ggagcgcaga gacucgccgc ucugggcgac acagccuggg auuucggguc uaucggaggc     1860

guguuuaaua gcauugggcg cgcuguccau caaguguucg gaggcgccuu ucggacgcug     1920

uucgggggca uguccuggau cacccaggga cuuaugggcg cccuccugcu guggaugggg     1980

guuaacgcua gagaccgcuc uauugcgcuc gccuuucugg cuacaggagg cgugcuuguc     2040

uuccuggcca ccaaugugca cgcc                                            2064


<210>  9899
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9899
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcauuacg       60

cggcgcggcu ccgcguacua uauguaccug gauagaucug acgcugggaa ggccaucagc      120

uuugccacca cacuuggagu uaacaaaugc caugugcaga uuauggaucu gggccacaug      180

ugugacgcua ccauguccua ugagugcccc augcucgaug aaggggugga gcccgacgau      240

gucgacuguu ggugcaauac aaccucuaca ugggugguuu acggaaccug ucaucacaag      300

aaaggcgaag cccggcgcag cagacgggcu gugacgcugc cgucccauuc uacccgcaaa      360

cuucaaacaa gaagccagac cuggcuggag ucccgggaau auacaaagca ccucaucaag      420

gucgagaacu ggauuuuccg caaucccggg uuugcccugg uggcgguugc caucgcuugg      480

cugcucggaa gcucuaccuc ccagaaggug aucuaccugg ucaugauucu ucugaucgcc      540

ccugcuuaua gcauuagaug caucggcgug ucuaaccggg auuucgugga gggcaugucc      600

gggggaacgu gggucgacgu ugugcucgaa cacggcgggu gugugacagu cauggcccag      660

gauaaaccca ccguggacau ugagcugguu acaaccaccg ugagcaauau ggcggaaguc      720

cgcucuuacu gcuacgaggc cuccaucagc gauauggcuu ccgacucuag auguccuaca      780

caaggagagg ccuaucuuga caagcagagc gauacgcagu acgugugcaa acggacccug      840

guugaccgcg gcugggggaa cggauguggc cucuuuggca aggggucccu ggugacaugc      900

gcuaaauuua ccugcagcaa gaagaugaca ggaaaaucua uucagcccga aaaucuggag      960

uauagaauca ugcucuccgu ccauggcagc caacacucug ggaugaucgg auacgaaacc     1020

gaugaggacc gggccaaggu ggaagucacg ccuaacagcc cgagagccga ggcuacacug     1080

ggcggguuug gaucccuugg ccuggauugu gagccccgca ccggccucga cuucucugau     1140

cuguauuacc uuaccaugaa uaacaaacau uggcuggugc acaaggaaug guuucacgac     1200

auuccucucc ccuggcaugc gggggccgau acaggaaccc cucacuggaa uaacaaagag     1260

gcucugguug aguucaagga cgcccaugcc aaacggcaga caguggucgu gcugggcagc     1320

caggaagggg cuguucacac ggcccucgcg ggagcucugg aagccgagau ggauggcgcc     1380

aaggggagac uuuucuccgg ccaucugaag ugccgccuca aaauggacaa gcugcggcuu     1440

aaaggaguga gcuauucucu guguaccgcu gccuucacau uuaccaaggu ccccgcugag     1500

acacuccacg gcaccgugac gguggaaguu caguacgccg ggaccgacgg accgugcaaa     1560

aucccugucc aaauggcggu ggauaugcag acacugaccc ccgugggcag acugauuaca     1620

gccaauccug ucaucaccga guccacggaa aacagcaaga ugaugcucga gcuggacccu     1680

cccuucgggg auucuuacau ugugaucgga guuggcgaca agaaaaucac acaccauugg     1740

caccgcuccg ggagcacccu uggcaaggcu uucuccacaa cccugaaagg agcccagcgg     1800

cucgcugccc ugggcgauac cgcgugggac uuugggucua uuggaggcgu guucaauagc     1860

aucgggagag ccguccauca aguguuugga ggcgcuuucc gcacacuguu ugggggcaug     1920

uccuggauua cgcagggacu uaugggcgcc cuccugcugu ggaugggggu uaacgcucgg     1980

gauagaucua ucgcccucgc cuuccuggcu accggaggcg ugcuugucuu ucuggcgaca     2040

aacgugcacg cc                                                         2052


<210>  9900
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9900
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcguuacg       60

cggcgcggcu ccgcguacua uauguaccug gauagaaacg acgcugggga ggccauuucu      120

uuucccacca cacuuggaau gaauaagugc uauauccaga uuauggaucu gggccauaug      180

ugugacgcca ccaugagcua cgaaugcccu augcucgaug agggggugga accggacgau      240

guggacuguu ggugcaacac aaccuccaca ugggucgugu auggaaccug ucaccauaaa      300

aagggcgagg cucggcgcuc uagacgggcc guuacgcugc ccagccacuc cacccgcaaa      360

cuucaaacaa gaucucagac cuggcuggaa agccgggagu acacaaagca ucucauccgc      420

guggagaauu ggauuuucag aaacccuggg uuugcucugg ccgcggccgc uaucgccugg      480

cugcucggau ccagcaccuc ucagaaaguc aucuaucugg ugaugauucu ucugaucgcu      540

cccgccuacu ccauucggug caucggcguu agcaaucgcg auuucgugga aggcaugucu      600

gggggaacgu gggucgacgu ggugcucgag cacggcgggu gugucacagu uauggcgcag      660

gauaagccua ccguggacau ugaacuggug acaaccaccg ucuccaacau ggccgaggug      720

agaagcuacu gcuaugaggc uucuaucucc gauauggcca gcgacucccg gugucccaca      780

caaggagagg cuuaccuuga caagcagucu gauacgcagu auguuugcaa acgcacccug      840

guggacagag gcugggggaa uggauguggc cucuuuggca aggggagccu ggucacaugc      900

gccaaauucg ccugcuccaa gaaaaugacc ggaaagagca uucagccuga aaaccuggaa      960

uaccggauca ugcucucugu gcacggcucc caacauagcg ggaugaucgu uaaugauaca     1020

ggacacgaga ccgacgaaaa cagagcuaag guggagauua cgcccaauuc uccccgcgcg     1080

gaggccacac ugggcggguu uggaagccuu ggccuggauu gugaaccucg gaccggccuc     1140

gacuucuccg aucuguauua ccuuaccaug aacaauaaac auuggcuggu ccacaaggag     1200

ugguuucacg acaucccccu cccuuggcau gcuggggccg auacaggaac cccgcacugg     1260

aacaauaaag aagcccuggu ggaguucaag gacgcucaug ccaaaagaca gacagucgug     1320

guucugggcu cucaggaagg ggcggugcac acggcucucg ccggagcccu ggaggcugag     1380

auggauggcg ccaaggggcg ccuuagcucc ggccaucuga aaugccggcu caagauggac     1440

aagcugagac uuaaaggagu cagcuauucu cuguguaccg cugccuuuac auucaccaag     1500

auuccggcgg aaacacucca cggcaccgug acgguugagg ugcaguacgc cgggaccgac     1560

ggaccuugca aaguccccgc ucaaauggcc guggauaugc agacacugac cccugugggc     1620

cgccugauca cagcuaaccc cguuauuacc gaauccacgg agaacagcaa gaugaugcuc     1680

gaacuggauc ccccuuuugg ggacucuuac aucgucaucg gagugggcga gaaaaagauu     1740

acacaccauu ggcaccgguc cgggagcacc cuuggcaagg ccuucuccac aacccugaaa     1800

ggagcgcaga gacucgccgc ucugggcgac accgccuggg auuucggguc uaucggaggc     1860

guguuuaaua gcauugggcg cgcuguccau caaguguucg gaggcgccuu ucggacacug     1920

uucgggggca uguccuggau cacgcaggga cuuaugggcg cccuccugcu guggaugggg     1980

guuaacgcua gagaccgcuc uauugcgcuc gccuuucugg cuaccggagg cgugcuuguc     2040

uuccuggcca caaaugugca cgcc                                            2064


<210>  9901
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9901
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccguuacccg gcgcggcagc gccuacuaua uguaccugga cagaaacgau      120

gcuggggagg ccaucuccuu ccccacaacg cuuggaauga auaaauguua uauucagauc      180

auggaccugg gccacaugug cgaugcuacc augucuuacg aauguccuau gcucgacgag      240

gggguggaac cggaugacgu ggauugcugg uguaacacaa ccagcacaug ggucguguau      300

ggaaccugcc aucacaagaa aggcgaggcc cggcgcucca gacgggcggu uacacugccc      360

ucucauagca cccgcaaacu ucaaacgaga ucccagaccu ggcuggaaag ccgggaguac      420

acaaaacacc ucauucgcgu ggagaauugg aucuuuagaa acccuggcuu cgcccuggcu      480

gccgcugcca ucgcguggcu gcucgggucu uccaccagcc agaaggucau uuaucuggug      540

augauccuuc ugauugcccc cgcuuacucu auccggugua uuggaguuuc caaucgcgac      600

uuuguggaag gcaugagcgg gggaacaugg gucgauaucg ugcucgagca uggcgggugc      660

gugaccguca uggcccagga caagccuacg guugauauug aacuggugac aaccacagug      720

ucuaacaugg cugaggucag auccuacugu uaugaggcca gcaucuccga cauggccucu      780

gauagccggu gccccaccca aggagaggcu uaccuugaca aacaguccga cacccaguau      840

guguguaagc gcacacuggu ugauagaggc uggggcaaug ggugcggacu cuucggcaaa      900

gggagccugg ugacgugcgc gaaguuugcc uguucuaaaa agaugaccgg aaaguccauc      960

cagccugaaa accuggaaua ccggauuaug cucagcgucc acggcucuca acacagcggg     1020

augaucguga augacacagg acaugagacc gaugaaaaca gagcuaaagu ugagauuaca     1080

cccaauuccc cgcgcgccga ggccacccug ggcggcuucg ggucucuugg acuggacugc     1140

gaaccucgga cgggccucga uuuuagcgac cuguauuacc uuacaaugaa caauaagcac     1200

uggcuggugc auaaagagug guuccacgau aucccccucc cuuggcacgc uggggccgac     1260

accggaacac cccauuggaa caauaaggaa gcgcuggucg aguuuaaaga ugcucacgcc     1320

aagagacaga ccguggucgu gcugggcucc caggaagggg ccguucauac cgcucucgcc     1380

ggcgcucugg aggccgagau ggacggagcg aaaggccgcc uuagcucugg gcaccugaag     1440

ugucggcuca agauggauaa acugagacuu aagggagugu ccuauagccu gugcacagcc     1500

gcuuucacgu uuaccaaaau uccggccgaa acacuccaug gcaccgucac aguggagguu     1560

caguacgcug ggaccgacgg accuuguaag gugcccgccc aaauggcggu cgacaugcag     1620

acgcugaccc cugugggccg ccugaucaca gccaaccccg ugaucaccga aucuacagag     1680

aacuccaaaa ugaugcucga acuggauccc ccuuucgggg acagcuacau uguuaucggc     1740

gucggagaga agaagauuac ccaccacugg caucgguccg gcucuacgcu ugggaaggcu     1800

uuuagcacaa cccugaaggg agcccagaga cucgcugccc ugggcgauac agccugggac     1860

uucgggucca ucggaggcgu guucaauucu auugggcgcg cugugcacca agucuuuggc     1920

ggagcguucc ggacccuguu uggcgggaug agcuggauca cccagggacu uaugggcgcc     1980

cuccugcugu ggaugggggu gaacgcuaga gaucgcucca ucgcccucgc cuuccuggcu     2040

acaggaggcg uucuuguguu ucuggccacg aauguccaug cg                        2082


<210>  9902
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9902
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccguuacccg gcgcggcagc gccuacuaua uguaccugga cagaaacgau      120

gcuggggagg ccaucuccuu ccccacaacg cuuggaauga auaaauguua uauucagauc      180

auggaccugg gccauaccug cgaugcuaca augucuuacg aauguccuau gcucgacgag      240

gggguggaac cggaugacgu ggauugcugg uguaacacca caagcaccug ggucguguau      300

ggaacaugcc accacaagaa aggcgaggcc cggcgcucca gacgggcggu uacccugccc      360

ucucauagca cgcgcaaacu ucaaaccaga ucccagacau ggcuggaaag ccgggaguac      420

accaaacacc ucauucgcgu ggagaauugg aucuuuagaa acccuggcuu cgcccuggcu      480

gccgcugcca ucgcguggcu gcucgggucu uccacaagcc agaaggucau uuaucuggug      540

augauccuuc ugauugcccc cgcuuacucu auccggugua uuggaguuuc caaucgcgac      600

uuuguggaag gcaugagcgg gggaaccugg gucgaugugg ugcucgagca uggcgggugc      660

gucacgguua uggcccagga caagccuaca guggauaucg aacuggugac cacaaccguc      720

ucuaacaugg cugaggugag auccuacugu uaugaggcca gcauuuccga cauggccucu      780

gauagccggu gccccaccca aggagaggcu uaccuugaca aacaguccga cacacaguau      840

guuuguaagc gcacgcuggu ggauagaggc uggggcaaug ggugcggacu cuucggcaaa      900

gggagccugg ugaccugcgc gaaguuugcc uguucuaaaa agaugacagg aaaguccauc      960

cagccugaaa accuggaaua ccggaucaug cucagcgucc acggcucuca acacagcggg     1020

augauuguua augacaccgg acaugagaca gaugaaaaca gagcuaaagu ggagaucacc     1080

cccaauuccc cgcgcgccga ggccacgcug ggcggcuucg ggucucuugg acuggacugc     1140

gaaccucgga caggccucga uuuuagcgac cuguauuacc uuaccaugaa caauaagcac     1200

uggcuggucc auaaagagug guuccacgau auuccccucc cuuggcacgc uggggccgac     1260

accggaacac cccauuggaa caauaaggaa gcgcuggugg aguuuaaaga ugcucacgcc     1320

aagagacaga ccgucguggu ucugggcucc caggaagggg ccgugcauac agcucucgcc     1380

ggcgcucugg aggccgagau ggacggagcg aaaggccgcc uuagcucugg gcaccugaag     1440

ugucggcuca agauggauaa acugagacuu aagggagucu ccuauagccu gugcacggcc     1500

gcuuucaccu uuacaaaaau cccggccgaa acccuccaug gcacagugac cguugaggug     1560

caguacgcug ggacggacgg accuuguaag guccccgccc aaauggcggu ggacaugcag     1620

acccugacac cugugggccg ccugauuacc gccaaccccg uuaucacaga aucuaccgag     1680

aacuccaaaa ugaugcucga acuggauccc ccuuucgggg acagcuacau cgucauuggc     1740

gugggagaga agaagaucac gcaccacugg caucgguccg gcucuacacu ugggaaggcu     1800

uuuagcacca cacugaaggg agcccagaga cucgcugccc ugggcgauac cgccugggac     1860

uucgggucca uuggaggcgu guucaauucu aucgggcgcg cuguccacca aguguuuggc     1920

ggagcguucc ggacccuguu uggcgggaug agcuggauua cacagggacu uaugggcgcc     1980

cuccugcugu ggaugggggu uaacgcuaga gaucgcucca ucgcccucgc cuuccuggcu     2040

acgggaggcg ugcuugucuu ucuggccacc aaugugcaug cg                        2082


<210>  9903
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9903
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccaucacccg gcgcggcagc gccuacuaua uguaccugga cagauccgau      120

gcugggaaag ccauuucuuu cgcuacaacg cuuggaguua acaaguguca cgugcagauc      180

auggaccugg gccauaugug cgaugccacc augagcuaug agugucccau gcucgacgaa      240

gggguggagc ccgaugacgu cgauugcugg uguaauacaa ccuccacaug ggugguuuac      300

ggaaccugcc accauaaaaa gggcgaagcg cggcgcucua gacgggccgu gacacugccg      360

agccacucca cccgcaaacu ucaaacgaga agccagaccu ggcuggaguc ucgggaauau      420

acaaagcauc ucauuaaggu cgagaacugg aucuuucgca aucccggcuu cgcucuggug      480

gccguugcua ucgccuggcu gcucgggucc agcaccucuc agaaagugau uuaccugguc      540

augauccuuc ugauugcgcc ugccuauucc aucagaugua uuggagugag caaccgggac      600

uuuguggagg gcaugucugg gggaacaugg gucgauguug ugcucgaaca cggcgggugc      660

gugaccguca uggcucagga caagcccacg guggauaucg agcugguuac aaccacagug      720

uccaauaugg ccgaaguccg cagcuacugu uacgaggcuu ccauuucuga cauggccagc      780

gauuccagau gcccuaccca aggagaggcc uaucuugaca aacagagcga cacccaguac      840

guguguaagc ggacacuggu ugaucgcggc uggggcaacg ggugcggacu cuucggcaaa      900

gggucucugg ugacgugcgc uaaguuuacc uguuccaaga aaaugacagg aaagagcauc      960

cagcccgaaa aucuggagua uagaaucaug cucucugucc acggcagcca acauuccggg     1020

augauuggau acgaaaccga cgaggaucgg gcgaaagugg aagucacacc uaacucuccg     1080

agagccgagg cuacccuggg cggcuucggg agccuuggac uggacugcga gccccgcacg     1140

ggccucgauu uuuccgaccu guauuaccuu acaaugaaua acaagcacug gcuggugcau     1200

aaagaauggu uccacgauau cccucucccc uggcacgccg gggccgacac cggaaccccu     1260

cauuggaaua acaaggaggc ucugguugaa uuuaaagaug cccacgcgaa gcggcagaca     1320

guggucgugc ugggcagcca ggagggggcu guucauaccg cccucgccgg cgcucuggaa     1380

gccgagaugg acggagcuaa gggcagacuu uucucugggc accugaaaug ucgccucaag     1440

auggauaaac ugcggcuuaa gggagugucc uauagccugu gcacagccgc guuuacguuc     1500

accaaagucc ccgccgagac acuccauggc accgugacag uggaaguuca guacgcuggg     1560

accgacggac cguguaagau uccuguccaa auggccgugg acaugcagac gcugaccccc     1620

gugggcagac ugaucacagc uaauccuguc auuaccgagu cuacagaaaa cuccaagaug     1680

augcucgagc uggauccucc cuuuggggac agcuacaucg ugaucggcgu uggagauaaa     1740

aagauuaccc accacuggca ucgcuccggc ucuacgcuug ggaaagccuu cagcacaacc     1800

cugaagggag cgcagcggcu cgccgcucug ggcgacacag ccugggauuu cggguccauc     1860

ggaggcgugu uuaauucuau ugggagagcu guccaccaag uguucggcgg agccuuucgc     1920

acccuguucg gcgggaugag cuggaucacc cagggacuua ugggcgcccu ccugcugugg     1980

auggggguua acgcucggga cagauccauu gcgcucgccu uucuggcuac aggaggcgug     2040

cuugucuucc uggccacgaa cgugcaugcc                                      2070


<210>  9904
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9904
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccguuacccg gcgcggcagc gccuacuaua uguaccugga cagaaacgau      120

gcuggggagg ccaucuccuu ccccacaacg cuuggaauga auaaauguua uauucagauc      180

auggaccugg gccacaugug cgaugcuacc augucuuacg aauguccuau gcucgacgag      240

gggguggaac cggaugacgu ggauugcugg uguaacacaa ccagcacaug ggucguguau      300

ggaaccugcc aucacaagaa aggcgaggcc cggcgcucca gacgggcggu uacacugccc      360

ucucauagca cccgcaaacu ucaaacgaga ucccagaccu ggcuggaaag ccgggaguac      420

acaaaacacc ucauucgcgu ggagaauugg aucuuuagaa acccuggcuu cgcccuggcu      480

gccgcugcca ucgcguggcu gcucgggucu uccaccagcc agaaggucau uuaucuggug      540

augauccuuc ugauugcccc cgcuuacucu auccggugua uuggaguuuc caaucgcgac      600

uuuguggaag gcaugagcgg gggaacaugg gucgaugugg ugcucgagca uggcgggugc      660

gucaccguua uggcccagga caagccuacg guggauaucg aacuggugac aaccacaguc      720

ucuaacaugg cugaggugag auccuacugu uaugaggcca gcauuuccga cauggccucu      780

gauagccggu gccccaccca aggagaggcu uaccuugaca aacaguccga cacccaguau      840

guuuguaagc gcacacuggu ggauagaggc uggggcaaug ggugcggacu cuucggcaaa      900

gggagccugg ucacgugcgc gaaguuugcc uguucuaaaa agaugaccgg aaaguccauc      960

cagccugaaa accuggaaua ccggaucaug cucagcgugc acggcucuca acacagcggg     1020

augauuguua augacacagg acaugagacc gaugaaaaca gagcuaaagu ggagaucaca     1080

cccaauuccc cgcgcgccga ggccacccug ggcggcuucg ggucucuugg acuggacugc     1140

gaaccucgga cgggccucga uuuuagcgac cuguauuacc uuacaaugaa caauaagcac     1200

uggcuggucc auaaagagug guuccacgau auuccccucc cuuggcacgc uggggccgac     1260

accggaacac cccauuggaa caauaaggaa gcgcuggugg aguuuaaaga ugcucacgcc     1320

aagagacaga ccgucguggu ucugggcucc caggaagggg ccgugcauac cgcucucgcc     1380

ggcgcucugg aggccgagau ggacggagcg aaaggccgcc uuagcucugg gcaccugaag     1440

ugucggcuca agauggauaa acugagacuu aagggagucu ccuauagccu gugcacagcc     1500

gcuuucacgu uuaccaaaau cccggccgaa acacuccaug gcaccgugac aguugaggug     1560

caguacgcug ggaccgacgg accuuguaag guccccgccc aaauggcggu ggacaugcag     1620

acgcugaccc cugugggccg ccugauuaca gccaaccccg uuaucaccga aucuacagag     1680

aacuccaaaa ugaugcucga acuggauccc ccuuucgggg acagcuacau cgucauuggc     1740

gugggagaga agaagaucac ccaccacugg caucgguccg gcucuacgcu ugggaaggcu     1800

uuuagcacaa cccugaaggg agcccagaga cucgcugccc ugggcgauac agccugggac     1860

uucgggucca uuggaggcgu guucaauucu aucgggcgcg cuguccacca aguguuuggc     1920

ggagcguucc ggacccuguu uggcgggaug agcuggauua cccagggacu uaugggcgcc     1980

cuccugcugu ggaugggggu uaacgcuaga gaucgcucca ucgcccucgc cuuccuggcu     2040

acaggaggcg ugcuugucuu ucuggccacg aaugugcaug cg                        2082


<210>  9905
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9905
augaccagcg ugggcaucgu cgggcugcuc cugacaacgg ccauggcugc cguuacccuu       60

cccucccacu cuacaagaaa gcugcagacc cggagccaaa cauggcucga gucccgcgaa      120

uacaccaaac aucugauuag aguggagaac uggaucuucc ggaauccugg auuugcgcuu      180

gcugccgccg cuauugccug gcugcucggc ucuagcacau cccagaaggu gaucuaucug      240

gucaugauuc ugcucaucgc uccggccuac ucuauccgcu gcauuggggu gagcaacaga      300

gacuucguug aaggaauguc cggcgggacc uggguggaua ucguccugga gcacggaggc      360

ugugugacgg uuauggcgca ggacaaaccc accguggaua uugaacuugu cacaaccaca      420

gugagcaaua uggccgaggu gcggucuuau ugcuacgagg cuuccaucag cgacauggcc      480

ucugauuccc gcuguccuac ccagggcgag gcuuaucugg acaagcaaag cgauacgcag      540

uacgucugca aaagaacacu cguugaccgg ggguggggaa acggcugugg gcuguuugga      600

aagggcucuc uugugaccug cgccaaguuc gcguguucca aaaagaugac agggaaaagc      660

auucagcccg aaaaucugga auaucgcauc augcucuccg ugcauggauc ucagcacagc      720

ggcaugauug ucaacgauac cggccaugag accgacgaga auagagccaa gguggaaauc      780

acaccuaaca gcccccgggc ugaggccacg cuugggggau uuggcucccu ggggcucgau      840

ugcgaaccuc gcaccggacu ggacuucucu gaucuuuacu accugacaau gaauaacaaa      900

cacuggcucg uucacaagga gugguuucau gacaucccgc ugcccuggca cgcuggcgcc      960

gacaccggga caccucauug gaauaacaag gaagcccuug uggaguucaa agaugcucac     1020

gcgaagagac aaaccgucgu gguucuggga ucccaggagg gcgccgugca cacggcucuc     1080

gccggcgccc uggaagcuga gauggacggg gccaaaggac ggcugagcuc uggccaucuc     1140

aagugucgcc ugaaaaugga uaagcugaga cugaaagggg ucagcuauuc ccucugcaca     1200

gcggcuuuua ccuucaccaa gauucccgcc gaaacacugc acggaaccgu gacagucgag     1260

gugcaguacg ccggcacgga cgggccuugu aagguucccg cucagauggc cguggauaug     1320

caaacccuua caccggucgg ccggcugauc accgcuaauc cugugauuac agaaucuacc     1380

gagaacagca aaaugaugcu cgagcuggau cccccuuuug gagacuccua uaucguuauu     1440

ggcguggggg aaaagaaaau cacgcaucac uggcauagaa gcggaucuac caucggcaag     1500

gccuucgagg cgacaguccg cggggccaaa cggauggcug ugcugggaga caccgccugg     1560

gauuuuggcu ccguuggggg cgcucucaau agccugggaa agggcauuca ccagaucuuc     1620

ggggccgcgu ucaagucucu uuuuggaggc auguccuggu ucagccagau ucugaucggg     1680

acacuccuga uguggcuggg acuuaacacc aaaaacggcu ccauuucucu caugugccug     1740

gcccucgggg gcgugcugau cuuucugagc acggcugucu ccgcc                     1785


<210>  9906
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9906
augaccagcg ugggcaucgu cgggcugcuc cugacaacgg ccauggcugc cguuacccuu       60

cccucccacu cuacaagaaa gcugcagacc cggagccaaa cauggcucga gucccgcgaa      120

uacaccaaac aucugauuag aguggagaac uggaucuucc ggaauccugg auuugcgcuu      180

gcugccgccg cuauugccug gcugcucggc ucuagcacau cccagaaggu gaucuaucug      240

gucaugauuc ugcucaucgc uccggccuac ucuauccgcu gcauuggggu gagcaacaga      300

gacuucguug aaggaauguc cggcgggacc uggguggaug ucgugcugga gcacggaggc      360

uguguuacgg ugauggcgca ggacaaaccc accgucgaua ucgaacuugu gacaaccaca      420

gugagcaaua uggccgaggu ccggucuuau ugcuacgagg cuuccauuag cgacauggcc      480

ucugauuccc gcuguccuac ccagggcgag gcuuaucugg acaagcaaag cgauacgcag      540

uacguuugca aaagaacacu cguggaccgg ggguggggaa acggcugugg gcuguuugga      600

aagggcucuc uugugaccug cgccaaguuc gcguguucca aaaagaugac agggaaaagc      660

auccagcccg aaaaucugga auaucgcauu augcucuccg uccauggauc ucagcacagc      720

ggcaugaucg ugaacgauac cggccaugag accgacgaga auagagccaa gguugaaauu      780

acaccuaaca gcccccgggc ugaggccacg cuugggggau uuggcucccu ggggcucgau      840

ugcgaaccuc gcaccggacu ggacuucucu gaucuuuacu accugacaau gaauaacaaa      900

cacuggcucg ugcacaagga gugguuucau gacaucccgc ugcccuggca cgcuggcgcc      960

gacaccggga caccucauug gaauaacaag gaagcccuug ucgaguucaa agaugcucac     1020

gcgaagagac aaaccguggu ugugcuggga ucccaggagg gcgccgucca cacggcucuc     1080

gccggcgccc uggaagcuga gauggacggg gccaaaggac ggcugagcuc uggccaucuc     1140

aagugucgcc ugaaaaugga uaagcugaga cugaaagggg ugagcuauuc ccucugcaca     1200

gcggcuuuua ccuucaccaa gauccccgcc gaaacacugc acggaaccgu cacaguggag     1260

guucaguacg ccggcacgga cgggccuugu aaggugcccg cucagauggc cgucgauaug     1320

caaacccuua caccgguggg ccggcugauu accgcuaauc cuguuaucac agaaucuacc     1380

gagaacagca aaaugaugcu cgagcuggau cccccuuuug gagacuccua uauugugauc     1440

ggcgucgggg aaaagaaaau uacgcaucac uggcauagaa gcggaucuac caucggcaag     1500

gccuucgagg cgacagugcg cggggccaaa cggauggcug ugcugggaga caccgccugg     1560

gauuuuggcu ccguuggggg cgcucucaau agccugggaa agggcaucca ccagauuuuc     1620

ggggccgcgu ucaagucucu uuuuggaggc auguccuggu ucagccagau ccugauuggg     1680

acacuccuga uguggcuggg acuuaacgcc aaaaacggcu ccaucucucu caugugccug     1740

gcucucgggg gcguccugau uuuucugagc accgccgugu ccgcu                     1785


<210>  9907
<211>  1773
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9907
augaccagca ucggcauuau cgggcugcug cugacaacgg ccauggcugc cgugacccuu       60

cccucccacu cuacaagaaa gcugcagacc cggagccaaa cauggcucga gucccgcgaa      120

uacaccaaac aucugauuaa ggucgagaac uggaucuuca gaaauccugg auuugcgcuu      180

guugcugugg ccauugccug gcugcucggc ucuagcacau cccagaaagu gaucuaucug      240

gucaugaucc ugcucauugc uccggccuac ucuauccggu gcauuggggu gagcaaccgc      300

gacuucguug aaggaauguc cggcgggacc uggguggaug ucgugcugga gcacggaggc      360

uguguuacgg ugauggcuca ggacaagccc accgucgaua ucgaacuugu gacaaccaca      420

gugagcaaua uggccgaggu cagaucuuau ugcuacgagg cguccauuag cgacauggcc      480

ucugauuccc gguguccuac ccagggcgag gcuuaucugg acaaacaaag cgauacgcag      540

uacguuugca agcgcacacu cguggacaga ggguggggaa acggcugugg gcuguuugga      600

aagggcucuc uugugaccug cgccaaauuc acauguucca agaaaaugac cggaaagagc      660

auccagcccg aaaaucugga auaucggauu augcucuccg uccauggguc ucagcacagc      720

ggcaugaucg gcuacgagac cgaugaggac cgcgcuaaag uggaaguuac accuaacucc      780

cccagagccg aggcgacgcu cgggggauuu ggcagccugg ggcuggauug cgaaccucgg      840

accggacugg acuucucuga ucuuuacuau cugacaauga auaacaagca uuggcucgug      900

cacaaggagu gguuucacga caucccgcug cccuggcaug ccggcgcuga caccgggaca      960

ccucacugga auaacaaaga agcccuuguc gaguucaagg augcucaugc caaacgccaa     1020

accgugguug ugcugggauc ccaggagggc gccguccaca cggcucucgc gggcgcccug     1080

gaagcugaga uggacggggc caagggaaga cuguuuagcg gccaccucaa augucggcug     1140

aagauggaua aacuucgccu gaagggggug ucuuacagcc ucugcacagc cgcuuucacc     1200

uuuaccaagg uccccgccga aacacugcau ggaaccguga caguugaggu gcaguaugcg     1260

ggcacggacg ggccuuguaa aauucccguc cagauggcug uggauaugca aacccuuaca     1320

ccgguuggca gacugaucac cgccaauccu gugauuacag aauccaccga gaacucuaag     1380

augaugcucg agcuggaucc cccuuucgga gacagcuaca ucgucauugg cgugggggac     1440

aaaaagauca cgcaccauug gcaccggucc ggaagcacca ucggcaaagc cuuugaagcu     1500

acagugagag gggccaagcg cauggcuguu cugggagaua ccgccuggga cuucggcucu     1560

gucgggggcg uguucaauuc ccucggaaag ggcauucauc agaucuuugg ggcggccuuc     1620

aaaagccugu uuggaggcau gucuugguuc ucccagauuc uuaucgggac acugcucgug     1680

uggcugggac ugaacaccaa gaauggcagc auuucccuua cgugccucgc ucucgggggc     1740

gucaugaucu uucugucuac agccgugagc gcu                                  1773


<210>  9908
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9908
augaccagcg ugggcaucgu cgggcugcuc cugacaacgg ccauggcugc cguuacccuu       60

cccucccacu cuacaagaaa gcugcagacc cggagccaaa cauggcucga gucccgcgaa      120

uacaccaaac aucugauuag aguggagaac uggaucuucc ggaauccugg auuugcgcuu      180

gcugccgccg cuauugccug gcugcucggc ucuagcacau cccagaaggu gaucuaucug      240

gucaugauuc ugcucaucgc uccggccuac ucuauccgcu gcauuggggu gagcaacaga      300

gacuucguug aaggaauguc cggcgggacc uggguggaug ucgugcugga gcacggaggc      360

uguguuacgg ugauggcgca ggacaaaccc accgucgaua ucgaacuugu gacaaccaca      420

gugagcaaua uggccgaggu ccggucuuau ugcuacgagg cuuccauuag cgacauggcc      480

ucugauuccc gcuguccuac ccagggcgag gcuuaucugg acaagcaaag cgauacgcag      540

uacguuugca aaagaacacu cguggaccgg ggguggggaa acggcugugg gcuguuugga      600

aagggcucuc uugugaccug cgccaaguuc gcguguucca aaaagaugac agggaaaagc      660

auccagcccg aaaaucugga auaucgcauu augcucuccg uccauggauc ucagcacagc      720

ggcaugaucg ugaacgauac cggccaugag accgacgaga auagagccaa gguugaaauu      780

acaccuaaca gcccccgggc ugaggccacg cuugggggau uuggcucccu ggggcucgau      840

ugcgaaccuc gcaccggacu ggacuucucu gaucuuuacu accugacaau gaauaacaaa      900

cacuggcucg ugcacaagga gugguuucau gacaucccgc ugcccuggca cgcuggcgcc      960

gacaccggga caccucauug gaauaacaag gaagcccuug ucgaguucaa agaugcucac     1020

gcgaagagac aaaccguggu ugugcuggga ucccaggagg gcgccgucca cacggcucuc     1080

gccggcgccc uggaagcuga gauggacggg gccaaaggac ggcugagcuc uggccaucuc     1140

aagugucgcc ugaaaaugga uaagcugaga cugaaagggg ugagcuauuc ccucugcaca     1200

gcggcuuuua ccuucaccaa gauccccgcc gaaacacugc acggaaccgu cacaguggag     1260

guucaguacg ccggcacgga cgggccuugu aaggugcccg cucagauggc cgucgauaug     1320

caaacccuua caccgguggg ccggcugauu accgcuaauc cuguuaucac agaaucuacc     1380

gagaacagca aaaugaugcu cgagcuggau cccccuuuug gagacuccua uauugugauc     1440

ggcgucgggg aaaagaaaau uacgcaucac uggcauagaa gcggaucuac caucggcaag     1500

gccuucgagg cgacagugcg cggggccaaa cggauggcug ugcugggaga caccgccugg     1560

gauuuuggcu ccguuggggg cgcucucaau agccugggaa agggcaucca ccagauuuuc     1620

ggggccgcgu ucaagucucu uuuuggaggc auguccuggu ucagccagau ccugauuggg     1680

acacuccuga uguggcuggg acuuaacacc aaaaacggcu ccaucucucu caugugccug     1740

gcccucgggg gcguccugau uuuucugagc acggcugugu ccgcc                     1785


<210>  9909
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9909
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggcugu gacccugccu agccacucca caagaaagcu gcaaacgcgg      120

ucucagaccu ggcuggaaag ccgcgaguac acaaaacauc ucauuagagu ggaaaacugg      180

aucuuccgga auccgggauu ugcccuggcc gcugccgcua uugccuggcu ucugggcucc      240

ucuaccagcc agaaggucau cuaucucgug augauccugc ugauugcgcc cgccuacucc      300

auccgcugca uugggguuag caacagagac uucguggagg gaaugucugg cgggacaugg      360

gucgauaucg ugcucgaaca cggaggcugu guuaccguga uggcucagga caaaccuaca      420

gucgauauug agcuggugac cacgaccgug uccaauaugg ccgagguccg gagcuauugc      480

uacgaggcuu cuaucuccga cauggccagc gauucucgcu gucccacaca aggcgaagcg      540

uaucuugaca agcaguccga uacccaguac guuugcaaaa gaacacuggu ggaccggggg      600

uggggaaacg gcugugggcu cuuuggaaag ggcagccugg ugaccugcgc caaguucgcu      660

uguuccaaaa agaugacggg gaaaucuauu cagccugaaa aucuugagua ucgcaucaug      720

cugagcgucc auggauccca acacagcggc augaucguga acgauacagg ccaugagacc      780

gacgaaaaua gagccaaggu ugagauuaca cccaacucuc cucgggcuga agccacccuc      840

gggggauuug gcucccuggg gcuggauugc gagccgcgca ccggacucga cuucagcgau      900

cuguacuacc uuacaaugaa uaacaaacac uggcuggugc acaaggaaug guuucaugac      960

aucccccucc cuuggcacgc cggcgcugac acggggacac cccauuggaa uaacaaggag     1020

gcgcuggucg aguucaaaga ugcccacgcu aagagacaga cagugguugu gcuuggaucu     1080

caggaaggcg ccguccacac cgcccuggcu ggcgcccucg aggcggaaau ggacggggcu     1140

aaaggacggc ugagcuccgg ccaucugaag ugucgccuca aaauggauaa gcugagacuu     1200

aaaggggugu cuuauagccu gugcacagcc gccuuuaccu ucacgaagau uccugcugag     1260

acacuccacg gaaccgucac cguggaaguu caguacgccg gcacagacgg gcccuguaag     1320

gugccggcuc aaauggccgu cgauaugcag acccugacac cugugggccg gcuuaucacg     1380

gcgaaucccg uuauuaccga guccacagag aacagcaaaa ugaugcugga acucgacccu     1440

cccuuuggag auucuuauau cgugaucggc gucggggaga agaaaauuac ccaucacugg     1500

cauagauccg gaagcacaau cggcaaggcc uucgaagcua ccgugcgcgg ggccaaacgg     1560

auggcugugc ugggagacac ggccugggau uucggcucug uugggggcgc gcuuaauucc     1620

cucggaaagg gcauucacca gaucuuuggg gccgcuuuca agagccuguu uggaggcaug     1680

uccugguucu cucaaauucu uaucgggacc cugcucaugu ggcugggacu gaacaccaaa     1740

aacggcagca ucucccuuau gugccucgcc cucgggggcg uccugauuuu ucugucuacc     1800

gcugugagcg cc                                                         1812


<210>  9910
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9910
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggcugu gacccugccu agccacucca caagaaagcu gcaaacgcgg      120

ucucagaccu ggcuggaaag ccgcgaguac acaaaacauc ucauuagagu ggaaaacugg      180

aucuuccgga auccgggauu ugcccuggcc gcugccgcua uugccuggcu ucugggcucc      240

ucuaccagcc agaaggucau cuaucucgug augauccugc ugauugcgcc cgccuacucc      300

auccgcugca uugggguuag caacagagac uucguggagg gaaugucugg cgggacaugg      360

gucgaugugg uucucgaaca cggaggcugu gugaccguca uggcucagga caaaccuaca      420

guggauaucg agcuggugac cacgaccguc uccaauaugg ccgagguucg gagcuauugc      480

uacgaggcuu cuauuuccga cauggccagc gauucucgcu gucccacaca aggcgaagcg      540

uaucuugaca agcaguccga uacccaguac gugugcaaaa gaacacuggu ggaccggggg      600

uggggaaacg gcugugggcu cuuuggaaag ggcagccugg ugaccugcgc caaguucgcu      660

uguuccaaaa agaugacggg gaaaucuauc cagccugaaa aucuugagua ucgcauuaug      720

cugagcgucc auggauccca acacagcggc augaucguua acgauacagg ccaugagacc      780

gacgaaaaua gagccaaggu ggagaucaca cccaacucuc cucgggcuga agccacccuc      840

gggggauuug gcucccuggg gcuggauugc gagccgcgca ccggacucga cuucagcgau      900

cuguacuacc uuacaaugaa uaacaaacac uggcuggucc acaaggaaug guuucaugac      960

auuccccucc cuuggcacgc cggcgcugac acggggacac cccauuggaa uaacaaggag     1020

gcgcuggugg aguucaaaga ugcccacgcu aagagacaga ccguuguggu ccuuggaucu     1080

caggaaggcg ccgugcacac cgcccuggcu ggcgcccucg aggcggaaau ggacggggcu     1140

aaaggacggc ugagcuccgg ccaucugaag ugucgccuca aaauggauaa gcugagacuu     1200

aaaggggucu cuuauagccu gugcacagcc gccuuuaccu ucacgaagau cccugcugag     1260

acacuccacg gaaccgugac cguugaagug caguacgccg gcacagacgg gcccuguaag     1320

gucccggcuc aaauggccgu ggauaugcag acccugacac cuguuggccg gcuuauuacg     1380

gcgaaucccg ugaucaccga guccacagag aacagcaaaa ugaugcugga acucgacccu     1440

cccuuuggag auucuuauau ugucaucggc gugggggaga agaaaaucac ccaucacugg     1500

cauagauccg gaagcacaau uggcaaggcc uucgaagcua ccgugcgcgg ggccaaacgg     1560

auggcuguuc ugggagacac ggccugggau uucggcucug ucgggggcgc gcuuaauucc     1620

cucggaaagg gcauccacca gauuuuuggg gccgcuuuca agagccuguu uggaggcaug     1680

uccugguucu cucaaauccu uauugggacc cugcucaugu ggcugggacu gaacgccaaa     1740

aacggcagca ucucccuuau gugccucgcu cucgggggcg ugcugaucuu ucugucuaca     1800

gccgugagcg cc                                                         1812


<210>  9911
<211>  1800
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9911
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggcugu gacccugccu agccacucca caagaaaacu ucaaacgcgg      120

ucucagaccu ggcuggaaag ccgcgaguac acaaagcauc ucauuaaggu ggaaaacugg      180

aucuucagaa auccgggauu ugcccugguc gccguggcua uugccuggcu ucugggcucc      240

ucuaccagcc agaaaguuau cuaucucgug augauccugc ugauugcucc cgccuacucc      300

auccggugca uuggggucag caaccgcgac uucguggagg gaaugucugg cgggacaugg      360

guugaugugg uccucgaaca cggaggcugu gugaccguga uggcgcagga caagccuaca      420

gucgauaucg agcugguuac cacgaccgug uccaauaugg ccgaggugag aagcuauugc      480

uacgaagccu cuauuuccga cauggcuagc gauucucggu gucccacaca aggcgaggcu      540

uaucuugaca aacaguccga uacccaguac gucugcaagc gcacacuggu ggacagaggg      600

uggggaaacg gcugugggcu cuuuggaaag ggcagccugg uuaccugcgc caaauucacg      660

uguuccaaga aaaugacagg gaagucuauc cagccugaaa aucuugagua ucggauuaug      720

cugagcgugc auggauccca acacagcggc augaucggcu acgagaccga ugaagaccgc      780

gcgaaagucg aggugacacc caacucuccu agagccgaag cuacccucgg gggauuuggc      840

ucccuggggc uggauugcga gccgcggacc ggacucgacu ucagcgaucu guacuaucuu      900

acaaugaaua acaagcauug gcugguucac aaggaauggu uucacgacau cccccucccu      960

uggcaugccg gcgcugacac ggggacaccc cacuggaaua acaaagaggc ccugguggag     1020

uucaaggaug cccaugcuaa acgccagacc gucguggucc uuggaucuca ggaaggcgcg     1080

gugcacaccg cccuggcugg cgcccucgag gccgaaaugg acggggcuaa gggaagacug     1140

uuuagcggcc accugaaaug ucggcucaag auggauaaac ugcgccuuaa ggggguuucc     1200

uacucucugu gcacagccgc guucaccuuu acgaaggugc cugcugagac acuccaugga     1260

accgucaccg uggaaguuca guaugccggc acagacgggc ccuguaaaau uccggugcaa     1320

auggccgucg auaugcagac ccugacaccu gugggcagac uuaucacggc uaaucccgug     1380

auuaccgaga gcacagagaa cuccaagaug augcuggaac ucgacccucc cuucggagau     1440

agcuacaucg uuauuggcgu cggggacaaa aagaucaccc accauuggca ccggucugga     1500

uccacaaucg gcaaagccuu cgaggcuacc gugagagggg ccaagcgcau ggcggugcug     1560

ggagauacgg ccugggacuu uggcagcguc gggggcgugu ucaauucucu gggaaagggc     1620

auucaucaga ucuuuggggc ugccuucaaa ucccucuuug gaggcaugag cugguucucc     1680

caaauucuga ucgggacccu ucugguuugg cucggacuga acacaaagaa uggcucuauu     1740

agccugaccu gccuugcucu cgggggcgug augaucuuuc uguccacagc cgucucugcg     1800


<210>  9912
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9912
auggccauca gcggcgugcc cguccugggg uucuuuauua ucgcuguucu cauguccgcc       60

caggagucuu gggcggcugu gacccugccu agccacucca caagaaagcu gcaaacgcgg      120

ucucagaccu ggcuggaaag ccgcgaguac acaaaacauc ucauuagagu ggaaaacugg      180

aucuuccgga auccgggauu ugcccuggcc gcugccgcua uugccuggcu ucugggcucc      240

ucuaccagcc agaaggucau cuaucucgug augauccugc ugauugcgcc cgccuacucc      300

auccgcugca uugggguuag caacagagac uucguggagg gaaugucugg cgggacaugg      360

gucgaugugg uucucgaaca cggaggcugu gugaccguca uggcucagga caaaccuaca      420

guggauaucg agcuggugac cacgaccguc uccaauaugg ccgagguucg gagcuauugc      480

uacgaggcuu cuauuuccga cauggccagc gauucucgcu gucccacaca aggcgaagcg      540

uaucuugaca agcaguccga uacccaguac gugugcaaaa gaacacuggu ggaccggggg      600

uggggaaacg gcugugggcu cuuuggaaag ggcagccugg ugaccugcgc caaguucgcu      660

uguuccaaaa agaugacggg gaaaucuauc cagccugaaa aucuugagua ucgcauuaug      720

cugagcgucc auggauccca acacagcggc augaucguua acgauacagg ccaugagacc      780

gacgaaaaua gagccaaggu ggagaucaca cccaacucuc cucgggcuga agccacccuc      840

gggggauuug gcucccuggg gcuggauugc gagccgcgca ccggacucga cuucagcgau      900

cuguacuacc uuacaaugaa uaacaaacac uggcuggucc acaaggaaug guuucaugac      960

auuccccucc cuuggcacgc cggcgcugac acggggacac cccauuggaa uaacaaggag     1020

gcgcuggugg aguucaaaga ugcccacgcu aagagacaga caguuguggu ccuuggaucu     1080

caggaaggcg ccgugcacac cgcccuggcu ggcgcccucg aggcggaaau ggacggggcu     1140

aaaggacggc ugagcuccgg ccaucugaag ugucgccuca aaauggauaa gcugagacuu     1200

aaaggggucu cuuauagccu gugcacagcc gccuuuaccu ucacgaagau cccugcugag     1260

acacuccacg gaaccgugac cguugaagug caguacgccg gcacagacgg gcccuguaag     1320

gucccggcuc aaauggccgu ggauaugcag acccugacac cuguuggccg gcuuauuacg     1380

gcgaaucccg ugaucaccga guccacagag aacagcaaaa ugaugcugga acucgacccu     1440

cccuuuggag auucuuauau ugucaucggc gugggggaga agaaaaucac ccaucacugg     1500

cauagauccg gaagcacaau uggcaaggcc uucgaagcua ccgugcgcgg ggccaaacgg     1560

auggcuguuc ugggagacac ggccugggau uucggcucug ucgggggcgc gcuuaauucc     1620

cucggaaagg gcauccacca gauuuuuggg gccgcuuuca agagccuguu uggaggcaug     1680

uccugguucu cucaaauccu uauugggacc cugcucaugu ggcugggacu gaacaccaaa     1740

aacggcagca ucucccuuau gugccucgcc cucgggggcg ugcugaucuu ucugucuacc     1800

gcugugagcg cc                                                         1812


<210>  9913
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9913
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcgcgguu       60

acgcugcccu cccauucuac ccggaaacuu cagacacgca gccaaaccug gcuggagucc      120

agagaauaca caaaacaccu cauucgggug gagaacugga ucuuucgcaa uccuggcuuc      180

gcucuggccg ccgcugccau ugcuuggcuu cuggggucua gcaccuccca gaaggugauc      240

uaucucguca ugauucugcu gaucgccccg gcguacucua ucagaugcau uggagugagc      300

aaccgggauu uuguugaagg cauguccggg ggaacauggg uggacaucgu ccucgagcau      360

ggcgggugug ugaccguuau ggcccaggau aaacccacgg uggacauuga acuggucacc      420

acaaccguga gcaauauggc ugaggugcgc ucuuauugcu acgaggccuc caucagcgau      480

auggcuucug acuccagaug uccuacacag ggagaagccu aucuugauaa gcaaagcgac      540

acccaguacg ucugcaaacg gacgcugguu gaucgcggcu ggggcaacgg guguggacuc      600

uucggcaagg ggucucuggu gacaugcgcg aaguuugccu guuccaaaaa gaugaccgga      660

aaaagcauuc agcccgagaa ucuugaauau agaaucaugc uguccgugca cggcucucag      720

cauagcggga ugauugucaa cgacacagga cacgagaccg augagaaucg ggcuaaggug      780

gaaaucaccc cuaacagccc ccgcgccgag gcuacacucg gcggcuucgg gucccuggga      840

cuggacugcg aaccuagaac gggccucgau uuuucugacc uguacuaccu uaccaugaau      900

aacaaacacu ggcugguuca uaaggagugg uuccacgaca ucccgcuccc cuggcaugcc      960

ggggccgaua caggaacccc ucacuggaau aacaaggaag cucuggugga guuuaaagac     1020

gcgcacgcca agcggcaaac agucgugguu cuuggcuccc aggagggggc ugugcauacc     1080

gcccuggccg gagcucucga agccgagaug gauggcgcga aaggccgccu gagcucuggg     1140

caccugaagu guagacucaa aauggacaag cugcggcuua aaggagucag cuauucccug     1200

ugcacggcug ccuucacauu uaccaagauu cccgccgaaa cccuccaugg cacagugacc     1260

gucgaggugc aguacgcugg gacagaugga ccuuguaagg uucccgccca gauggcugug     1320

gacaugcaaa cgcugacccc ggucggcaga cuuaucacag ccaauccugu gauuaccgaa     1380

ucuacagaga acagcaaaau gaugcuggag cucgaucccc cuuucgggga cuccuauauc     1440

guuauuggcg ugggagaaaa gaaaaucacc caccauuggc accgcagcgg cucuacgauc     1500

gggaaggcgu uugaggccac cguccgggga gcuaaaagaa uggccgugcu gggcgauaca     1560

gcuugggacu ucggguccgu gggaggcgcc cugaauagcc ucgggaaggg cauucaccag     1620

aucuucggag cggccuuuaa gucucuguuc ggcgggaugu ccugguuuag ccagauucuu     1680

aucggaaccc ugcucaugug gcugggccug aacacaaaga acggguccau uucucuuaug     1740

ugccucgcuc ugggaggcgu ucugaucuuc cucagcaccg ccgucuccgc u              1791


<210>  9914
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9914
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcgcgguu       60

acgcugcccu cccauucuac ccggaaacuu cagacacgca gccaaaccug gcuggagucc      120

agagaauaca caaagcaccu cauucgggug gagaacugga ucuuucgcaa uccuggcuuc      180

gcucuggccg ccgcugccau ugcuuggcuu cuggggucua gcaccuccca gaaggugauc      240

uaucucguca ugauucugcu gaucgccccg gcguacucua ucagaugcau uggagugagc      300

aaccgggauu uuguugaagg cauguccggg ggaacauggg uggacgucgu gcucgagcau      360

ggcgggugug uuaccgugau ggcccaggau aaacccacgg ucgacaucga acuggugacc      420

acaaccguga gcaauauggc ugagguccgc ucuuauugcu acgaggccuc cauuagcgau      480

auggcuucug acuccagaug uccuacacag ggagaagccu aucuugauaa gcaaagcgac      540

acccaguacg uuugcaaacg gacgcuggug gaucgcggcu ggggcaacgg guguggacuc      600

uucggcaagg ggucucuggu gacaugcgcg aaguuugccu guuccaaaaa gaugaccgga      660

aaaagcaucc agcccgagaa ucuugaauau agaauuaugc uguccgucca cggcucucag      720

cauagcggga ugaucgugaa cgacacagga cacgagaccg augagaaucg ggcuaagguu      780

gaaauuaccc cuaacagccc ccgcgccgag gcuacacucg gcggcuucgg gucccuggga      840

cuggacugcg aaccuagaac gggccucgau uuuucugacc uguacuaccu uaccaugaau      900

aacaaacacu ggcuggugca uaaggagugg uuccacgaca ucccgcuccc cuggcaugcc      960

ggggccgaua caggaacccc ucacuggaau aacaaggaag cucuggucga guuuaaagac     1020

gcgcacgcca agcggcaaac agugguugug cuuggcuccc aggagggggc uguccauacc     1080

gcccuggccg gagcucucga agccgagaug gauggcgcga aaggccgccu gagcucuggg     1140

caccugaagu guagacucaa aauggacaag cugcggcuua aaggagugag cuauucccug     1200

ugcacggcug ccuucacauu uaccaagauc cccgccgaaa cccuccaugg cacagucacc     1260

guggagguuc aguacgcugg gacagaugga ccuuguaagg ugcccgccca gauggcuguc     1320

gacaugcaaa cgcugacccc ggugggcaga cuuauuacag ccaauccugu uaucaccgaa     1380

ucuacagaga acagcaaaau gaugcuggag cucgaucccc cuuucgggga cuccuauauu     1440

gugaucggcg ucggagaaaa gaaaauuacc caccauuggc accgcagcgg cucuacgauc     1500

gggaaggcgu uugaggccac cgugcgggga gcuaaaagaa uggccgugcu gggcgauaca     1560

gcuugggacu ucggguccgu uggaggcgcc cugaauagcc ucgggaaggg cauccaccag     1620

auuuucggag cggccuuuaa gucucuguuc ggcgggaugu ccugguuuag ccagauccuu     1680

auuggaaccc ugcucaugug gcugggccug aacgcuaaaa acggguccau cucucuuaug     1740

ugccucgccc ugggaggcgu ccugauuuuc cucagcacag cuguguccgc c              1791


<210>  9915
<211>  1779
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9915
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcgcgguu       60

acgcugcccu cccauucuac ccggaaacuu cagacacgca gccaaaccug gcuggagucc      120

agagaauaca caaaacaccu cauuaaggug gagaacugga ucuuucggaa uccuggcuuc      180

gcucuggugg ccgucgccau ugcuuggcuu cuggggucua gcaccuccca gaaagugauc      240

uaucucguua ugauucugcu gaucgccccg gcuuacucua uccgcugcau uggagugagc      300

aacagagauu uugucgaagg cauguccggg ggaacauggg uggacguugu gcucgagcau      360

ggcgggugug ucaccgugau ggcccaggau aagcccacgg uggacaucga acuggucacc      420

acaaccguua gcaauauggc ggaggugcgg ucuuauugcu acgaggccuc cauuagcgau      480

auggcuucug acucccgcug uccuacacag ggagaagccu aucuugauaa acaaagcgac      540

acccaguacg ugugcaagag aacgcugguc gaucggggcu ggggcaacgg guguggacuc      600

uucggcaagg ggucucuggu gacaugcgcu aaauuuaccu guuccaagaa aaugacagga      660

aagagcaucc agcccgagaa ucuugaauau cgcauuaugc uguccguuca cggcucucag      720

cauagcggga ugaucggaua cgagaccgac gaggauagag ccaaagugga agucaccccu      780

aacagccccc gggcggaggc cacacucggc ggcuucgggu cccugggacu ggacugcgaa      840

ccucgcacgg gccucgauuu uucugaccug uacuaucuua ccaugaauaa caagcacugg      900

cuggugcaca aggagugguu ccaugacauu ccgcuccccu ggcacgcugg ggccgauaca      960

ggaaccccuc auuggaauaa caaagaagcu cugguugagu uuaaggacgc ccacgccaaa     1020

agacaaacag uggucgugcu uggcucccag gagggggcug uccacaccgc gcuggccgga     1080

gcucucgaag ccgagaugga uggcgccaag ggccggcugu ucagcgggca ucugaaaugu     1140

cgccucaaga uggacaaacu gagacuuaag ggagugucuu acagccugug cacggcugcc     1200

uuuacauuca ccaagguucc cgcggaaacc cuccacggca cagugaccgu cgaggugcag     1260

uaugcuggga cagauggacc uuguaaaauc cccguucaga uggccgugga caugcaaacg     1320

cugaccccgg ucggccggcu uaucacagcc aauccuguga uuaccgaauc cacagagaac     1380

ucuaagauga ugcuggagcu cgaucccccu uuuggggaca gcuacaucgu gauuggcguu     1440

ggagauaaaa agaucaccca ucacuggcau agauccggca gcacgauugg gaaggcuuuc     1500

gaagccaccg uccgcggagc uaagcggaug gccgugcugg gcgacacagc gugggauuuc     1560

gggucugugg gaggcgucuu uaauucccug gggaagggca uccaccagau cuucggagcc     1620

gcuuuuaaaa gccucuucgg cgggaugucu ugguuuuccc agauucugau cggaacccuu     1680

cugguguggc ucggccugaa cacaaagaau gggagcauuu cccugaccug ccuugcccuc     1740

ggaggcguua ugaucuuccu gucuacggcu gugagcgcc                            1779


<210>  9916
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9916
auggacugga ccuggauccu guuccucgug gccgcugcca caagagucca cagcgcgguu       60

acgcugcccu cccauucuac ccggaaacuu cagacacgca gccaaaccug gcuggagucc      120

agagaauaca caaaacaccu cauucgggug gagaacugga ucuuucgcaa uccuggcuuc      180

gcucuggccg ccgcugccau ugcuuggcuu cuggggucua gcaccuccca gaaggugauc      240

uaucucguca ugauucugcu gaucgccccg gcguacucua ucagaugcau uggagugagc      300

aaccgggauu uuguugaagg cauguccggg ggaacauggg uggacgucgu gcucgagcau      360

ggcgggugug uuaccgugau ggcccaggau aaacccacgg ucgacaucga acuggugacc      420

acaaccguga gcaauauggc ugagguccgc ucuuauugcu acgaggccuc cauuagcgau      480

auggcuucug acuccagaug uccuacacag ggagaagccu aucuugauaa gcaaagcgac      540

acccaguacg uuugcaaacg gacgcuggug gaucgcggcu ggggcaacgg guguggacuc      600

uucggcaagg ggucucuggu gacaugcgcg aaguuugccu guuccaaaaa gaugaccgga      660

aaaagcaucc agcccgagaa ucuugaauau agaauuaugc uguccgucca cggcucucag      720

cauagcggga ugaucgugaa cgacacagga cacgagaccg augagaaucg ggcuaagguu      780

gaaauuaccc cuaacagccc ccgcgccgag gcuacacucg gcggcuucgg gucccuggga      840

cuggacugcg aaccuagaac gggccucgau uuuucugacc uguacuaccu uaccaugaau      900

aacaaacacu ggcuggugca uaaggagugg uuccacgaca ucccgcuccc cuggcaugcc      960

ggggccgaua caggaacccc ucacuggaau aacaaggaag cucuggucga guuuaaagac     1020

gcgcacgcca agcggcaaac agugguugug cuuggcuccc aggagggggc uguccauacc     1080

gcccuggccg gagcucucga agccgagaug gauggcgcga aaggccgccu gagcucuggg     1140

caccugaagu guagacucaa aauggacaag cugcggcuua aaggagugag cuauucccug     1200

ugcacggcug ccuucacauu uaccaagauc cccgccgaaa cccuccaugg cacagucacc     1260

guggagguuc aguacgcugg gacagaugga ccuuguaagg ugcccgccca gauggcuguc     1320

gacaugcaaa cgcugacccc ggugggcaga cuuauuacag ccaauccugu uaucaccgaa     1380

ucuacagaga acagcaaaau gaugcuggag cucgaucccc cuuucgggga cuccuauauu     1440

gugaucggcg ucggagaaaa gaaaauuacc caccauuggc accgcagcgg cucuacgauc     1500

gggaaggcgu uugaggccac cgugcgggga gcuaaaagaa uggccgugcu gggcgauaca     1560

gcuugggacu ucggguccgu uggaggcgcc cugaauagcc ucgggaaggg cauccaccag     1620

auuuucggag cggccuuuaa gucucuguuc ggcgggaugu ccugguuuag ccagauccuu     1680

auuggaaccc ugcucaugug gcugggccug aacacaaaga acggguccau cucucuuaug     1740

ugccucgcuc ugggaggcgu ccugauuuuc cucagcaccg ccguguccgc u              1791


<210>  9917
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9917
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccgccguuac ccugcccagc cacuccacac ggaaacuuca gacgcgcucu      120

caaaccuggc uggagagcag agaauacaca aagcaucuca uccgggugga gaacuggauu      180

uuccgcaauc cuggcuuugc ucuggccgcu gccgcgaucg ccuggcuucu gggguccucu      240

accagccaga aagugauuua ucucgucaug auccugcuga ucgcuccggc cuacuccauu      300

agauguaucg gagugagcaa ccgggacuuc guugaaggca ugucuggggg aacaugggug      360

gauauugucc ucgagcacgg cggcugcgug accguuaugg cucaggacaa gcccacagug      420

gauaucgaac uggucaccac gaccgugucc aauauggccg aggugcgcag cuauuguuac      480

gaggcgucua uuuccgacau ggccagcgau ucuagaugcc cuacacaggg ggaggcuuau      540

cuugacaaac aauccgauac ccaguacguc uguaagcgga cacugguuga ccgcggaugg      600

ggcaacgggu gcggacucuu uggcaagggg agccugguga ccugugccaa auucgcuugc      660

uccaagaaaa ugacgggaaa gucuauccag cccgaaaauc uugaauauag aauuaugcug      720

agcgugcaug gcucccagca cagcggcaug aucgucaacg auacagggca ugagaccgac      780

gagaaucggg ccaaagugga aaucacaccu aacucucccc gcgccgaggc uacccucgga      840

ggcuuugggu cccugggacu ggauugugaa ccuagaaccg gccucgacuu cagcgaucug      900

uacuaccuua caaugaauaa caagcacugg cugguucaca aggagugguu ucaugacauu      960

ccgcuccccu ggcacgcggg ggccgacacg ggaaccccuc auuggaauaa caaagaagcu     1020

cugguggagu ucaaggaugc ccacgccaaa cggcaaacag ucgugguucu uggcucucag     1080

gagggcgcug ugcacaccgc ccuggcgggg gcucucgaag ccgagaugga cggagccaag     1140

ggccgccuga gcuccgggca ucugaaaugc agacucaaga uggauaaacu gcggcuuaag     1200

ggagucucuu auagccugug uacagcugcc uuuaccuuca cgaagauccc cgcugaaaca     1260

cuccacggca ccgugaccgu cgaggugcag uacgccggga cagacggccc uugcaaaguu     1320

cccgcgcaga uggccgugga uaugcaaacc cugacaccgg ucggaagacu uauuacggcu     1380

aauccuguga ucaccgaauc cacagagaac agcaagauga ugcuggagcu cgaucccccu     1440

uuuggcgacu cuuauauugu uaucggggug ggagagaaaa agaucaccca ucacuggcau     1500

cgcuccggca gcacaauugg gaaagccuuc gaagcuaccg uccggggagc caagagaaug     1560

gcggugcugg gcgacacggc cugggauuuu gggucugugg gcggagcucu caauucccug     1620

ggcaagggga uccaccagau uuucggagcc gcuuucaaaa gccuguuugg cgggaugucc     1680

ugguucucuc agauccuuau uggaacccug cucauguggc ugggccugaa cacaaagaac     1740

gggagcaucu cccuuaugug ccucgcccug ggcggaguuc ugaucuuucu cucuaccgcc     1800

gucagcgcu                                                             1809


<210>  9918
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9918
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccgccguuac ccugcccagc cacuccacac ggaaacuuca gacgcgcucu      120

caaaccuggc uggagagcag agaauacaca aagcaucuca uccgggugga gaacuggauu      180

uuccgcaauc cuggcuuugc ucuggccgcu gccgcgaucg ccuggcuucu gggguccucu      240

accagccaga aagugauuua ucucgucaug auccugcuga ucgcuccggc cuacuccauu      300

agauguaucg gagugagcaa ccgggacuuc guugaaggca ugucuggggg aacaugggug      360

gaugucgugc ucgagcacgg cggcugcguu accgugaugg cucaggacaa gcccacaguc      420

gauauugaac uggugaccac gaccgugucc aauauggccg agguccgcag cuauuguuac      480

gaggcgucua ucuccgacau ggccagcgau ucuagaugcc cuacacaggg ggaggcuuau      540

cuugacaaac aauccgauac ccaguacguu uguaagcgga cacuggugga ccgcggaugg      600

ggcaacgggu gcggacucuu uggcaagggg agccugguga ccugugccaa auucgcuugc      660

uccaagaaaa ugacgggaaa gucuauucag cccgaaaauc uugaauauag aaucaugcug      720

agcguccaug gcucccagca cagcggcaug auugugaacg auacagggca ugagaccgac      780

gagaaucggg ccaaaguuga aaucacaccu aacucucccc gcgccgaggc uacccucgga      840

ggcuuugggu cccugggacu ggauugugaa ccuagaaccg gccucgacuu cagcgaucug      900

uacuaccuua caaugaauaa caagcacugg cuggugcaca aggagugguu ucaugacauc      960

ccgcuccccu ggcacgcggg ggccgacacg ggaaccccuc auuggaauaa caaagaagcu     1020

cuggucgagu ucaaggaugc ccacgccaaa cggcaaacag ugguugugcu uggcucucag     1080

gagggcgcug uccacaccgc ccuggcgggg gcucucgaag ccgagaugga cggagccaag     1140

ggccgccuga gcuccgggca ucugaaaugc agacucaaga uggauaaacu gcggcuuaag     1200

ggagugucuu auagccugug uacagcugcc uuuaccuuca cgaagauucc cgcugaaaca     1260

cuccacggca ccgucaccgu ggagguucag uacgccggga cagacggccc uugcaaagug     1320

cccgcgcaga uggccgucga uaugcaaacc cugacaccgg ugggaagacu uaucacggcu     1380

aauccuguua uuaccgaauc cacagagaac agcaagauga ugcuggagcu cgaucccccu     1440

uuuggcgacu cuuauaucgu gauugggguc ggagagaaaa agaucaccca ucacuggcau     1500

cgcuccggca gcacaaucgg gaaagccuuc gaagcuaccg ugcggggagc caagagaaug     1560

gcggugcugg gcgacacggc cugggauuuu gggucuguug gcggagcucu caauucccug     1620

ggcaagggga uucaccagau cuucggagcc gcuuucaaaa gccuguuugg cgggaugucc     1680

ugguucucuc agauucuuau cggaacccug cucauguggc ugggccugaa cgccaagaac     1740

gggagcauuu cccuuaugug ccucgcccug ggcggagucc ugaucuuucu cucuacagcu     1800

gugagcgcg                                                             1809


<210>  9919
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9919
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccgccguuac ccugcccagc cacuccacac ggaaacuuca gacgcgcucu      120

caaaccuggc uggagagcag agaauacaca aagcaucuca ucaaagugga gaacuggauu      180

uuccggaauc cuggcuuugc ucugguggcc gucgcuaucg ccuggcuucu gggguccucu      240

accagccaga aggugauuua ucucguuaug auccugcuga ucgcgccggc cuacuccauu      300

cgcuguaucg gagugagcaa cagagacuuc gucgaaggca ugucuggggg aacaugggug      360

gauguugugc ucgagcacgg cggcugcguc accgugaugg cucaggacaa acccacagug      420

gauauugaac uggucaccac gaccguuucc aauauggccg aggugcggag cuauuguuac      480

gaggcuucua ucuccgacau ggccagcgau ucucgcugcc cuacacaggg ggaagcguau      540

cuugacaagc aauccgauac ccaguacgug uguaagagaa cacuggucga ccggggaugg      600

ggcaacgggu gcggacucuu uggcaaaggg agccugguga ccugugccaa guucacgugc      660

uccaaaaaga ugacaggaaa aucuauucag cccgagaauc uugaauaucg caucaugcug      720

agcguucaug gcucccagca cagcggcaug auuggguacg agaccgauga ggacagagcu      780

aagguggaag ucacaccuaa cucuccccgg gccgaggcua cccucggagg cuuugggucc      840

cugggacugg auugugaacc ucgcaccggc cucgacuuca gcgaucugua cuaucuuaca      900

augaauaaca agcauuggcu ggugcacaaa gagugguuuc acgacauccc gcuccccugg      960

caugccgggg ccgacacggg aaccccucac uggaauaaca aggaagcucu gguugaguuc     1020

aaagaugcgc augccaagag acaaacagug gucgugcuug gcucucagga gggcgcuguc     1080

cacaccgccc uggccggggc ucucgaagcc gagauggacg gagcgaaagg ccggcuguuu     1140

agcgggcacc ugaagugccg ccucaaaaug gauaagcuga gacuuaaggg aguguccuac     1200

ucucugugua cagcugccuu caccuuuacg aaaguucccg ccgaaacacu ccauggcacc     1260

gugaccgucg aggugcagua ugcugggaca gacggcccuu gcaagauccc cguucagaug     1320

gccguggaua ugcaaacccu gacaccgguc ggacggcuua uuacggcuaa uccugugauc     1380

accgaaagca cagagaacuc caaaaugaug cuggagcucg aucccccuuu cggcgacagc     1440

uacauuguga ucgggguugg agacaagaaa auuacccacc auuggcacag aucuggcucc     1500

acaaucggga aggccuuuga agcgaccguc cgcggagcca agcggauggc ugugcugggc     1560

gauacggccu gggacuucgg gagcgugggc ggagucuuca auucucuggg caaagggauc     1620

caucagauuu uuggagcugc cuucaagucc cucuuuggcg ggaugagcug guucucccag     1680

auccugauug gaacccuucu gguguggcuc ggccugaaca ccaaaaaugg gucuaucagc     1740

cugacaugcc uugcgcucgg cggaguuaug auuuuucugu ccacagccgu gucugcu        1797


<210>  9920
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9920
augggcaaga gaagcgccgg guccaucaug uggcuggcuu cucucgccgu ggucauugcg       60

ugcgcuggag ccgccguuac ccugcccagc cacuccacac ggaaacuuca gacgcgcucu      120

caaaccuggc uggagagcag agaauacaca aagcaucuca uccgggugga gaacuggauu      180

uuccgcaauc cuggcuuugc ucuggccgcu gccgcgaucg ccuggcuucu gggguccucu      240

accagccaga aagugauuua ucucgucaug auccugcuga ucgcuccggc cuacuccauu      300

agauguaucg gagugagcaa ccgggacuuc guugaaggca ugucuggggg aacaugggug      360

gaugucgugc ucgagcacgg cggcugcguu accgugaugg cucaggacaa gcccacaguc      420

gauauugaac uggugaccac gaccgugucc aauauggccg agguccgcag cuauuguuac      480

gaggcgucua ucuccgacau ggccagcgau ucuagaugcc cuacacaggg ggaggcuuau      540

cuugacaaac aauccgauac ccaguacguu uguaagcgga cacuggugga ccgcggaugg      600

ggcaacgggu gcggacucuu uggcaagggg agccugguga ccugugccaa auucgcuugc      660

uccaagaaaa ugacgggaaa gucuauucag cccgaaaauc uugaauauag aaucaugcug      720

agcguccaug gcucccagca cagcggcaug auugugaacg auacagggca ugagaccgac      780

gagaaucggg ccaaaguuga aaucacaccu aacucucccc gcgccgaggc uacccucgga      840

ggcuuugggu cccugggacu ggauugugaa ccuagaaccg gccucgacuu cagcgaucug      900

uacuaccuua caaugaauaa caagcacugg cuggugcaca aggagugguu ucaugacauc      960

ccgcuccccu ggcacgcggg ggccgacacg ggaaccccuc auuggaauaa caaagaagcu     1020

cuggucgagu ucaaggaugc ccacgccaaa cggcaaacag ugguugugcu uggcucucag     1080

gagggcgcug uccacaccgc ccuggcgggg gcucucgaag ccgagaugga cggagccaag     1140

ggccgccuga gcuccgggca ucugaaaugc agacucaaga uggauaaacu gcggcuuaag     1200

ggagugucuu auagccugug uacagcugcc uuuaccuuca cgaagauucc cgcugaaaca     1260

cuccacggca ccgucaccgu ggagguucag uacgccggga cagacggccc uugcaaagug     1320

cccgcgcaga uggccgucga uaugcaaacc cugacaccgg ugggaagacu uaucacggcu     1380

aauccuguua uuaccgaauc cacagagaac agcaagauga ugcuggagcu cgaucccccu     1440

uuuggcgacu cuuauaucgu gauugggguc ggagagaaaa agaucaccca ucacuggcau     1500

cgcuccggca gcacaaucgg gaaagccuuc gaagcuaccg ugcggggagc caagagaaug     1560

gcggugcugg gcgacacggc cugggauuuu gggucuguug gcggagcucu caauucccug     1620

ggcaagggga uucaccagau cuucggagcc gcuuucaaaa gccuguuugg cgggaugucc     1680

ugguucucuc agauucuuau cggaacccug cucauguggc ugggccugaa cacaaagaac     1740

gggagcauuu cccuuaugug ccucgcccug ggcggagucc ugaucuuucu cucuaccgcc     1800

gugagcgcu                                                             1809


<210>  9921
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9921
auggcaauaa gcggaguacc aguuuuaggu uucuuuauaa uagcugucuu aaugucagca       60

caggaaucau gggcaguaac uagaagaggg ucagccuauu auauguaccu agauagaaau      120

gacgcuggug aagcgauaag uuuuccaacc acuuuaggca ugaauaaaug cuauauacaa      180

auaauggacc uuggacauau gugcgaugcu acaaugucau acgaaugccc aauguuagau      240

gaagguguag aaccagauga cguagauugc ugguguaaua caacgaguac auggguagua      300

uauggaacuu gccaucauaa gaaaggagaa gcaagaagau caaggagagc aguuacauua      360

ccaucucauu cuacuagaaa acuacaaacc agaucucaaa cuugguuaga aaguagagaa      420

uacacaaaac aucuaauaag aguagaaaau uggaucuuua gaaauccagg auuugcauua      480

gcagcagcag caauagcaug gcuauuagga agcucaacaa gucaaaaagu aauauacuua      540

guaaugauac uucuaauagc accagcauau ucaauaagau guauaggugu aucaaauaga      600

gauuuugucg aaggaauguc aggugguaca uggguagaua uagucuuaga acacggagga      660

ugugucacag uuauggcuca agauaaacca acaguagaua uugagcuagu aacaacgaca      720

guaucaaaca uggccgaagu cagaucauac uguuaugaag caucaauaag cgacauggca      780

agugauagua gauguccgac acaaggagaa gcauaucuag acaaacaauc agauacacag      840

uauguaugca aaagaacauu aguagauaga ggcuggggaa acgguugcgg auuauuuggu      900

aaaggcagcc ucguaacaug cgcaaaauuu gcaugcucga agaaaaugac agguaaaagu      960

auacaaccag aaaaccuaga auacagaauc auguuaagug uucaugguag ucaacauagu     1020

ggaaugauag uuaacgauac aggacacgaa acugaugaaa auagagcaaa aguagaaaua     1080

acaccuaauu cuccaagagc agaagcaaca cuagggggau uugguucauu aggauuagac     1140

ugugaaccaa gaacagguuu agauuuuuca gauuuauauu accucacaau gaauaacaaa     1200

cauugguuag uacauaaaga augguuucau gauauuccau ugccuuggca ugccggugca     1260

gacacaggca caccacauug gaauaauaag gaagcacuug uggaauuuaa agaugcacau     1320

gcaaaaagac aaacaguugu aguacucggu ucacaagaag gagcaguaca caccgcauua     1380

gcaggcgcac ucgaggcaga aauggauggg gcaaaaggga gacuaaguag uggacaucug     1440

aaaugcagau uaaaaaugga caaacuacga cuaaaaggag uaucauauag ucuuugcaca     1500

gccgcguuca cguucacuaa aauaccugcu gaaacauuac acggaacggu aacuguagaa     1560

guacaauaug caggaacaga uggaccaugc aaaguuccug cgcaaauggc agucgacaug     1620

caaacauuaa caccaguagg gagguuaauu acagcuaauc caguuauaac agaaaguaca     1680

gagaauagua aaaugaugcu agaauuagau ccaccuuuug gggacucuua cauaguaaua     1740

ggaguaggag aaaagaaaau aacccaucau uggcaucgaa guggaaguac aauaggaaaa     1800

gcauuugagg caacaguaag aggagcaaaa agaauggcag uacuuggaga uacagccugg     1860

gacuucggca gugucggagg agcacuaaau aguuuaggaa aagguauaca ucaaauuuuu     1920

ggagccgccu uuaaaucauu auucggaggg auguccuggu uuagucaaau auuaauuggc     1980

acauuauuaa uguggcuagg auuaaauacc aaaaauggcu caauaucauu aauguguuua     2040

gcguuaggug gaguacuaau cuuuuugagc acggcaguca gugcc                     2085


<210>  9922
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9922
auggcuauaa gcggaguacc aguccuaggu uucuuuauaa ucgcugucuu aaugucagca       60

caagaaucgu gggccguaac cagaagagga agugcauauu auauguauuu agaccgaaau      120

gaugcaggcg aagcgauauc auuuccaacg acauuaggaa ugaauaaaug uuauauacaa      180

auaauggauu uaggacacac augcgaugca accaugucuu acgaaugucc aauguuagac      240

gaaggaguag aaccagauga cguagacugu ugguguaaua caaccaguac cuggguaguu      300

uacggaacau gucaucacaa gaaaggagaa gcaagaagaa guagacgagc aguaaccuug      360

ccaucacauu caacaagaaa auuacaaaca agaucacaaa caugguuaga aagucgagaa      420

uauacaaaac aucuaauaag aguagaaaac uggauauuua ggaacccugg auuugcacuc      480

gcagcagcug caauugcaug guugcuagga agcucuacuu cccaaaaagu uauuuauuua      540

guaaugauau uauuaauagc accagcauau ucaauuagau guaucggugu aucuaauaga      600

gacuuuguag aaggaaugag cggagguacu uggguagaug ucguauuaga acauggagga      660

uguguaacag uaauggccca agauaaacca acgguagaca uagaauuagu cacuacaacu      720

guuucaaaua uggcugaagu uagaucauac uguuaugaag caaguauauc agauauggcc      780

agugacucca gauguccaac ccaaggagaa gcauauuuag acaaacaaag ugacacacaa      840

uauguaugca aaagaacucu aguagauaga ggcuggggaa auggaugcgg auuauuugga      900

aaggggaguu uaguaacaug cgcaaaauuu gcaugcucaa agaaaaugac aggaaaaagu      960

auccaaccag aaaauuuaga auauagaaua auguuaucag uacauggauc gcagcacagc     1020

ggaaugauug uuaacgacac aggacaugaa acagaugaaa acagagcaaa aguugaaauu     1080

acaccaaacu caccaagagc agaagcgaca uuaggaggau uuggaucacu agguuuagau     1140

ugugaaccca gaacuggacu ugauuucagu gacuuauauu aucuuacgau gaauaauaaa     1200

cacuggcuag uucacaaaga augguuucau gauauaccac uuccauggca ugcaggagca     1260

gauaccggca caccucacug gaacaauaaa gaggcccuag uagaauuuaa agacgcucau     1320

gcaaaaagac agacuguugu gguauuaggc ucacaagaag gagcaguuca cacugcgcua     1380

gcaggggccu uagaggcaga aauggacggc gcaaaaggua gacucucaag cggacauuua     1440

aaaugcagac ugaaaaugga uaaauuacga cuaaaaggag uuaguuauag ccuauguacu     1500

gccgcauuua cuuucacaaa aauaccagca gaaacacugc auggaacugu aacgguagaa     1560

guacaauacg ccgguacaga cggaccuugu aaaguaccag cacaaauggc cguagauaug     1620

caaacacuaa cuccuguugg uagacuaaua acugcaaauc cuguaauaac agaaagcaca     1680

gaaaauucaa aaaugauguu agaauuagau ccaccuuuug gagauucaua uauaguaauc     1740

gggguuggag aaaagaaaau uacacaccau uggcaucgau caggaucaac aaucgguaaa     1800

gccuuugaag cgacaguaag aggugcaaaa agaauggcag uauuaggaga uacagcaugg     1860

gauuuugguu cgguaggagg agcauuaaau ucacuaggaa aagguauuca ccaaauauuu     1920

ggagcagccu uuaaaaguuu auuuggagga augaguuggu uuagucaaau uuugauagga     1980

accuuauuaa uguggcuugg acucaacgca aagaacggau caauuagcuu aaugugucua     2040

gcguuaggag gaguuuugau auuccuauca acagcaguaa gugcu                     2085


<210>  9923
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9923
auggccauau caggaguacc aguauuaggu uuuuucauaa uagcaguacu caugucagca       60

caagaaucau gggcgauaac aagaagagga ucagcauauu acauguauuu agauagauca      120

gaugcgggaa aagcaauuuc cuuugcaaca acauuaggug ugaacaaaug ccauguacaa      180

auuauggauc uuggucacau gugcgacgca acaaugucgu acgaaugccc uaugcuugac      240

gaaggaguug aaccagauga cgucgauugu ugguguaaca caaccucaac auggguuguc      300

uacgguacuu gucaucauaa aaaaggggaa gcaaggagau caagaagagc uguaacacua      360

ccaagucauu caacuagaaa auuacaaaca agaagccaaa cauggcuaga aucucgagaa      420

uauaccaaac acuuaauaaa aguagaaaac uggauauuua ggaauccagg auucgcauua      480

gucgccguag ccauugccug guuacuggga ucuaguacaa gccaaaaagu cauuuaccua      540

guaaugauac uuuuaauagc accagcauac ucaauucggu guauaggugu caguaacagg      600

gauuuuguug aagguaugag uggaggaaca uggguagaug uuguacucga acacggcgga      660

ugugucacag uaauggcaca agauaaacca acuguagaca uagaacuagu aacaacaacu      720

guaucaaaca uggcggaagu uagaucauac uguuaugaag caaguauauc agauauggca      780

ucagauucac gcugcccaac acaaggcgaa gcauauuuag auaaacaaag cgauacacaa      840

uauguaugca aacgaacacu aguagacaga ggcuggggca auggaugcgg auuauucggc      900

aaaggaaguc uuguaaccug ugcaaaauuu acaugcucaa aaaaaaugac agguaaaagu      960

auacaaccag aaaaucuaga auaucgaaua auguuaagcg uacauggauc acaacauucu     1020

ggaaugauug gauaugaaac ggaugaagau agagcaaaag uagaaguaac accaaauucc     1080

ccaagagcag aagccacacu agguggcuuu ggaucucuag gcuuagauug ugaaccaaga     1140

acuggauuag auuuuucaga uuuauauuau uugacaauga auaauaaaca uugguuggua     1200

cauaaagaau gguuucacga uauaccuuua ccauggcaug cuggagcaga cacaggaacc     1260

ccacauugga acaauaaaga agcauuaguc gaguucaaag augcccaugc aaaaagacaa     1320

acgguaguag uucuaggauc ccaagaagga gcgguacaua cagcacucgc aggagcacua     1380

gaagccgaaa uggauggugc uaaaggaaga uuauuuucag gacaucuuaa auguagauua     1440

aaaauggaua aauuaagacu uaaaggagua ucuuauaguc ucuguacugc ggcuuuuaca     1500

uuuacaaaag uaccagcaga aacguuacau gguacaguaa caguagaagu acaauacgca     1560

ggaacagacg guccauguaa aauuccugua caaauggcag uagauaugca aacucugaca     1620

ccaguaggua gauuaauaac agcuaauccu guaauaacag agucaaccga gaacucaaaa     1680

augaugcuag aauuagaucc accuuuugga gauucauaua uagucauagg cguaggagac     1740

aaaaaaauaa cacaucacug gcacagauca ggaaguacua uagggaaggc auuugaagca     1800

acgguaagag gcgcaaaaag aauggcagua uuaggagaua ccgcauggga uuuuggaagu     1860

guugguggug uguuuaacuc uuuagggaaa gggauacauc aaauauuugg agcagcauuu     1920

aaaagcuugu uuggagguau gucaugguuu ucacaaauac uaauaggaac auugcuagua     1980

ugguuaggau uaaauacuaa aaauggaagc auaagcuuaa caugcuuagc auuaggugga     2040

guaaugauau uuuuaucaac agcaguauca gca                                  2073


<210>  9924
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9924
auggcuauuu cugggguucc aguauuagga uucuuuauaa uagcaguauu aaugucagca       60

caagaaaguu gggcaguaac aagaagagga agugcauacu auauguauuu ggacagaaau      120

gacgcuggag aagccauaag uuuuccaaca acauugggca ugaacaaaug uuauauacaa      180

auaauggauu uaggacauau gugugaugca acaaugucau acgaaugucc uaugcuagau      240

gaaggagucg aaccagacga uguagauugu uggugcaaua caacuucaac auggguaguu      300

uauggaaccu gucaucauaa gaaaggggaa gcaagaagga guagaagggc aguaacuuua      360

cccucucaua gcacaagaaa auuacaaaca agaucacaaa cuuggcucga aagcagagaa      420

uauacuaaac auuuaauaag aguagaaaau uggauuuuca gaaauccagg auuugcucua      480

gcagcagcag caauagcaug guuacuuggu uccaguacaa gucaaaaagu aaucuaccuu      540

guaaugauac uucuuauugc uccagcguau ucaauaaggu guauuggagu aaguaacaga      600

gacuucguag aagguauguc uggaggcacg uggguagacg uaguauuaga acacgguggg      660

uguguaacug uaauggcuca agauaaacca acuguugaua ucgaauuagu gacaacaaca      720

guuucaaaca uggcugaagu acgaagcuac ugcuacgaag caucaauaag ugauauggca      780

agugauaguc gaugcccaac acaaggagaa gcauaucuag acaaacaaag ugauacacaa      840

uauguaugua aaagaacacu uguagauaga ggauggggaa augguugugg gcuauuuggc      900

aaagggagcu uagucaccug ugcaaaauuu gcuuguagca agaaaaugac aggaaaaagc      960

auacaaccag aaaaucuaga auauagaaua auguuaagug uccauggauc acaacauagu     1020

ggaaugauag uaaaugacac cggacacgaa acugacgaaa aucgagcaaa aguggaaaua     1080

acaccaaaua gucccagggc cgaagcuaca uuaggagguu uuggaucacu aggacuggau     1140

ugugaaccca ggacaggguu agauuucagc gauuuauauu auuuaacaau gaacaauaaa     1200

cauugguugg uacauaaaga augguuucau gauauaccau uacccuggca ugcuggugcu     1260

gauacaggca caccucauug gaacaauaaa gaagcauuag uagaauuuaa agaugcgcau     1320

gcaaaaagac aaacaguugu cguuuuagga agucaagaag gcgcaguaca uacugcucua     1380

gcaggagcau uagaagcuga aauggaugga gcaaaaggca gacugucaag uggacauuua     1440

aaaugucgac uaaaaaugga uaaauugcga uuaaaaggag uaucauauuc cuuaugcacu     1500

gcagcuuuua cauuuacaaa aauaccugca gaaacguuac auggaacagu uacaguagaa     1560

guucaauacg ccggaacaga uggaccaugu aaaguaccag cacaaauggc aguagacaug     1620

caaacauuaa caccugucgg acgacuaauc acagcaaauc caguuauuac agaaucaaca     1680

gaaaauagua aaaugaugcu agaauuagau ccaccauucg gagauucaua cauaguaaua     1740

ggaguuggag aaaagaaaau aacacaccau uggcaucgaa gcggaaguac aauaggaaaa     1800

gcguucgaag cuacaguccg uggagcaaaa agaauggcag uauuaggaga uaccgcaugg     1860

gauuuuggaa guguaggcgg agcauuaaau aguuuaggaa aagguauaca ccaaaucuuu     1920

ggagcagcau ucaaaucacu auuuggagga augagcuggu uuucgcagau acugauaggu     1980

acuuuauuaa uguggcuagg auuaaauacg aaaaacggua gcauaucauu gauguguuua     2040

gcacucggug gaguacuaau auuuuuaucu acagcuguau ccgcg                     2085


<210>  9925
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9925
auggauugga cauggauuuu auuucuaguc gcagcagcua caagaguaca cuccguaaca       60

agaagaggau cggcauacua uauguauuua gacagaaacg augcagggga agcaauauca      120

uuuccaacaa cucuugguau gaauaaaugu uacauacaga uaauggauuu aggacacaug      180

ugcgaugcca cuaugucgua cgagugccca auguuagacg aaggggugga gccggaugau      240

guagauugcu gguguaacac uaccucuaca uggguagucu auggaacaug ucaccacaag      300

aaaggagaag caagaagaag uagacgagca guaaccuuac caagucauuc aacaagaaaa      360

uuacaaacaa gaucacaaac augguuagaa ucaagggaau auacaaaaca uuuaauuaga      420

guagaaaacu ggauauuuag aaauccuggu uuugcuuugg cagcagccgc uaucgcuugg      480

uuauuaggaa gcagcacauc acaaaaagua auauauuugg uaaugauuuu acucauagcc      540

cccgcguaua guauucgaug uauaggugua ucuaauagag acuucguaga aggaauguca      600

ggaggaacuu ggguagacau cguauuagaa cacggaggau guguaacagu aauggcucaa      660

gacaaaccga caguagauau agaauuggua acaacaacug uuucuaauau ggcagaggua      720

cgaucauacu guuaugaagc aagcaucagu gauauggcuu cagacagccg auguccaaca      780

caaggugaag cauacuuaga uaaacaaagc gacacacaau auguaugcaa aagaacguua      840

guagauagag gcuggggaaa ugguugcggg uuauucggaa aaggaucauu gguaacaugc      900

gcaaaauuug cauguucaaa gaaaaugacu ggaaaaucua uacaaccuga aaaucuagaa      960

uauaggauaa uguuaagugu ucauggaucu caacacucag gaaugauagu aaaugacaca     1020

ggacacgaaa cggaugaaaa uagagccaaa guagaaauaa caccaaauuc accgagagca     1080

gaagcgacau ugggaggauu ugggucacua ggauuagauu gugaaccgag aaccggguua     1140

gauuuuucug aucucuauua ucuaacaaug aauaauaaac auugguuagu acacaaagaa     1200

ugguuccaug auauuccauu accuuggcac gcaggagccg auacagguac uccacacugg     1260

aacaauaaag aagcauuagu agaauuuaaa gacgcacaug caaaaagaca aacaguagua     1320

guauuaggaa gucaagaagg cgcaguacac accgcucuag caggagccuu agaagcagaa     1380

auggauggag caaaggguag auugucuuca ggccaucuaa aauguagacu aaaaauggau     1440

aaacuaagau uaaaaggagu aagcuacucu uuaugcacag cagcauuuac guuuacaaaa     1500

auaccagcag aaaccuuaca uggaacuguu acuguagaag uacaauaugc aggaacagau     1560

ggaccaugua aaguuccagc acaaauggca guagauaugc aaacauuaac accaguagga     1620

agauuaauua cagcaaaucc aguaauuaca gaaucaaccg aaaacucaaa aaugauguua     1680

gaauuagacc caccuuucgg agacucauau auaguaauag gaguaggaga aaagaaaauu     1740

acacaccauu ggcaccgauc cggaucaaca auaggaaaag cauucgaagc uacaguacga     1800

ggcgcaaaaa ggauggcagu cuuaggagau acagcauggg auuuuggaag cguaggagga     1860

gcacuuaacu ccuuagguaa aggcauacau caaauauuug gagcagcguu uaaaucauua     1920

uuugggggua ugucuugguu uagccaaaua cucauaggua ccuuauuaau guggcuagga     1980

cuuaauacua aaaacgguuc aauuucauua auguguuuag ccuuaggagg uguuuuaaua     2040

uuucuuagua cagcaguuuc agca                                            2064


<210>  9926
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9926
auggauugga cauggauauu auuuuuagug gcagcagcaa caagagugca cucagugacc       60

aggagaggaa gugcauauua uauguauuua gauagaaaug acgcgggaga agcaauauca      120

uucccaacua cguuaggaau gaauaaaugu uauauacaaa uaauggauuu aggucauaca      180

ugugaugcaa caaugucuua cgaauguccg auguuagacg aaggaguaga accagaugac      240

guagacugcu ggugcaauac aacaaguacu uggguaguau acggaacaug ccaucacaaa      300

aaaggagaag cuagaagauc ucgaagggca guaacuuuac caagucauuc uacacgaaaa      360

uugcaaacca ggucacaaac augguuagaa ucaagggaau acacaaaaca ucugauaaga      420

guagaaaacu ggauauuuag aaaucccggg uuugcauuag cugcagcagc aauagcaugg      480

cuuuuagguu caucaacauc ucaaaaagua auauaucuug ugaugauacu cuuaauugca      540

ccugcuuaca guauuaggug uauagguguc ucaaauagag acuuugucga agggaugagu      600

ggugguacau ggguugaugu ugucuuggaa caugguggau guguaacagu aauggcacaa      660

gauaaaccaa ccguagauau agaauuaguc acaacgacag uaucuaacau ggcagaaguu      720

agaucuuauu gcuaugaagc gucaauaucu gauauggccu cggacucaag augcccaaca      780

caaggcgaag cauaucuaga uaaacaauca gauacccagu auguauguaa aagaacauua      840

guugauagag guuggggaaa ugguugcgga cuauuuggca aaggaucauu aguaacaugu      900

gcaaaauucg cauguagcaa aaaaaugaca ggaaaaucaa uucaaccaga aaauuuggag      960

uauagaauaa ugcuaagugu gcacggaagc cagcauagug gaaugauugu aaaugauaca     1020

ggacaugaaa cugacgagaa uagagcgaaa guagaaauca caccaaauuc accaagagcu     1080

gaagcuacac uaggaggauu ugguaguuua ggccucgauu gugaaccaag aacaggauua     1140

gacuucuccg aucuauauua uuuaacaaug aauaauaaac acuggcuagu ucauaaagaa     1200

ugguuucaug auauaccuuu accuuggcau gcaggagcag auacaggaac uccucauugg     1260

aacaauaaag aagcacuagu agaauuuaaa gaugcacacg caaaaagaca aacaguaguc     1320

guacuaggaa gucaagaagg agcaguucau acagcuuuag caggagcauu agaagcagaa     1380

auggauggag caaaagggag auuaucauca ggacaccuaa aauguagauu aaaaauggau     1440

aaauuaagau uaaaaggagu aucauacuca uuguguacag cagcauuuac auuuacaaaa     1500

auaccagcag aaacauuaca uggaacaguc acgguugaag uacaauacgc cggaaccgac     1560

ggaccaugca aaguaccugc acaaauggca guagauaugc aaacacuaac accaguagga     1620

cgacuaauaa ccgcgaaucc aguaauaaca gaaaguacag aaaauagcaa aaugauguua     1680

gaacuagauc cuccauuugg agauucauac auaguaauug gagucgggga aaaaaagauu     1740

acucaccacu ggcaucgauc gggcaguaca auaggaaaag cauuugaagc aacaguacgc     1800

ggagcaaaaa gaauggcagu acuaggagac accgcauggg auuucgguuc aguaggugga     1860

gcauuaaauu cauuagguaa aggaauacac caaauauucg gagccgccuu uaaaaguuug     1920

uuuggcggaa ugaguugguu uagccaaaua uugaucggaa ccuuacuaau guggcugggu     1980

uuaaaugcaa aaaaugguag cauaagucua augugcuuag cacugggugg aguacuaaua     2040

uuuuuaucaa cggcaguauc agcg                                            2064


<210>  9927
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9927
auggauugga ccuggauauu auuuuuagua gcagcagcua cuagggugca uucaauaaca       60

agaagaggau cagcuuacua uauguauuua gacagaagcg acgcaggaaa agcaaucagc      120

uuugcuacaa cacuaggugu aaauaaaugu cauguacaaa uaauggauuu aggacauaug      180

ugugaugcaa caaugaguua cgaauguccu auguuagaug aaggugugga accugacgau      240

guagauuguu ggugcaauac uacuagcacu uggguagucu auggcacaug ucaucauaag      300

aaaggcgaag caaggagaag uagacgugca guaacauugc caucucacag uacacgaaaa      360

uuacaaacua gaucacagac auggcuugaa ucaagagaau auacaaaaca cuuaauaaaa      420

guagaaaauu ggauauucag aaauccuggg uucgcacuug uggcugucgc uauagcuugg      480

uuacuaggaa guaguacgag ucaaaaaguu auauaucuug uuaugauauu acuaauagca      540

ccagcauaca guauaagaug uauagggguu ucuaacagag acuuuguuga aggaauguca      600

gguggaacau ggguagacgu aguauuagaa cacggaggau guguaacugu aauggcacaa      660

gauaaaccaa cuguugauau cgaacucgua acaacaacag ugagcaauau ggcggaagug      720

agaucauacu guuaugaggc aucaaucuca gacauggcau cagauucaag auguccaacu      780

caaggugaag cguaucugga caaacaauca gacacacaau auguauguaa aaggacauua      840

guugauagag gaugggguaa uggaugcgga cuauucggaa aagggagcuu aguaaccugu      900

gcuaaauuua cauguaguaa aaagaugaca ggaaaaucaa uacaaccaga aaauuuagaa      960

uacagaauaa ugcuaucagu ccauggcagu caacacagcg gaaugauugg uuaugaaacg     1020

gacgaggaua gagcuaaagu ugaaguaacg ccuaauaguc cuagagcaga agcuacacua     1080

ggaggauuug gaucacuagg auuagacugc gaaccacgaa caggauuaga uuuuagugau     1140

uuauauuacc uaacgaugaa uaacaaacac ugguuaguac acaaagaaug guuucacgau     1200

auaccauuac cauggcaugc aggcgcugau acaggaacac cacauuggaa caauaaagag     1260

gcuuugguag aauuuaaaga cgcacaugcu aaaaggcaga caguaguagu auuaggauca     1320

caagaaggag cugugcauac agcacuugca ggagcacuag aagcagaaau ggacggggca     1380

aaaggacgau uguucucagg acauuuaaag uguagacuaa aaauggauaa acuaagacua     1440

aaagguguau cauauagccu auguacugcc gcauuuacgu uuacuaaagu cccagcagaa     1500

acucuacaug gaaccguaac ugucgaagug caauaugcag guacagaugg cccuuguaaa     1560

auaccaguuc aaauggcagu ggacaugcaa acauuaacac cgguaggaag auuaauaaca     1620

gcaaauccgg uuauaacgga aucaacagaa aauaguaaaa ugaugcuaga auuggaucca     1680

ccauuuggug auucauauau aguaauaggc guaggagaca agaaaauaac acaucacugg     1740

cauagaagcg gaucaacaau agguaaagca uuugaagcua cuguaagagg agcaaaaaga     1800

auggcagucc uaggugauac agcuugggac uuugguagcg uaggaggagu guuuaauuca     1860

cucggaaaag guauucacca aaucuuugga gccgcuuuua aaucauuauu cggagguaug     1920

agcugguucu cacaaauccu aauaggaaca cuauuagugu gguugggacu aaauacuaaa     1980

aaugguagca uaucacuaac auguuuagca uuaggagggg uuaugauauu ucuaaguaca     2040

gcuguaagcg ca                                                         2052


<210>  9928
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9928
auggauugga cauggauauu auuuuuagua gcagcagcaa cgagaguaca uaguguaaca       60

agacgagguu ccgcauacua cauguauuua gauagaaaug augcgggaga agccauauca      120

uucccgacga cauuaggaau gaauaaaugu uauauccaaa uaauggauuu aggacacaug      180

ugugaugcaa caaugucaua ugaaugucca auguuagacg aaggaguuga accugaugau      240

guagauuguu gguguaacac aacuagcacg uggguugucu auggaacaug ucaucauaag      300

aaaggagaag caagacggag uaggagagca guaacauugc caucacacag uacaagaaaa      360

cuacaaacac gaagucaaac augguuagag agccgagaau auacaaaaca ucuaauaaga      420

guagaaaauu ggauuuucag aaauccaggu uuugcauuag cagcagcagc aauagccugg      480

uuauuaggau caucaacaag ucaaaaagua auauauuuag ucaugaucuu auuaauagca      540

ccagcguauu caauacgaug uauuggagua ucuaacaggg auuucguaga agguauguca      600

ggaggaacau ggguagaugu aguacuugag cauggaggau gugugacagu aauggcacaa      660

gauaaaccaa cgguugauau agaauuagua acaacaacag uaucaaacau ggcagaagua      720

agaaguuauu gcuaugaagc aagcauauca gauauggcuu cugacagucg auguccaaca      780

caaggagaag cauauuuaga caaacaaucu gauacucaau augucugcaa aagaacauug      840

guagauaggg gguggggaaa uggaugugga uuguuuggaa aaggaagucu aguaacgugu      900

gcaaaauuug cuugcucgaa aaaaaugacu ggaaaaagua uacaaccaga aaaucuugaa      960

uauagaauaa uguuaagugu ucauggaagu caacauucag gaaugauugu aaaugauacu     1020

ggacaugaaa cggacgaaaa uagggccaaa guagaaauaa caccuaauag uccuagagcu     1080

gaagccacau uaggaggauu cggaucauua ggacucgacu gugaaccaag gacaggacua     1140

gauuucucug acuuguauua cuuaacaaug aauaauaaac auugguuagu acacaaagaa     1200

ugguuucacg auauaccacu accauggcau gcgggagcug auacagguac uccacacugg     1260

aauaauaaag aagcacucgu agaauuuaaa gacgcacacg caaaaagaca aacaguugua     1320

guauuagguu cacaagaggg cgcaguacau acagcauuag caggugcuuu agaagcugaa     1380

auggacggag caaaaggaag auuaucaagu ggacaccuaa aaugcagauu gaaaauggau     1440

aaauuacgau uaaaaggagu aucuuauagc cuuuguacug ccgcauuuac cuuuacaaaa     1500

auaccagcag aaacauuaca uggaacugua acaguagaag uacaauacgc uggcacugac     1560

ggaccaugua aaguaccggc ucaaauggcg guagauaugc aaacauuaac accaguaggu     1620

cgccuaauaa cagcaaaucc aguaauaacu gaaagcacug agaauuccaa aaugauguua     1680

gaauuggauc caccuuuugg agauucauac auaguaauug gaguagguga aaaaaaaaua     1740

acacaucacu ggcauagauc cggaucaaca auaggaaaag cauuugaagc aacaguucgu     1800

ggggcuaaaa gaauggcagu acuaggagau acagccuggg auuucggauc gguaggaggu     1860

gcauuaaacu cauuaggaaa agguauacau caaauauuug gagcagccuu caaaagucuc     1920

uuuggaggca ugaguugguu cagucaaaua uugauaggaa cucuauuaau guggcucggc     1980

uuaaauacaa aaaaugguuc aauuucauua auguguuuag cauuaggagg aguauuaauu     2040

uucuuaucaa ccgcaguauc cgcu                                            2064


<210>  9929
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9929
augggaaaaa gaucagcugg aaguauaaug ugguuagcau cauuagcugu aguaauagca       60

ugcgcaggag cuguaacaag aagaggaagc gcauauuaua uguaccuaga uagaaacgac      120

gcaggagaag cuaucucuuu uccaacaaca uuaggaauga auaaauguua uauacaaauu      180

auggauuuag gacauaugug cgacgcaaca augucauaug aauguccuau guuagacgaa      240

ggaguagaac cagacgaugu agauuguugg uguaauacaa caaguacuug gguaguuuau      300

ggcacaugcc aucauaagaa aggggaagcu cgaagaagca gacgagcagu aacauuacca      360

agucauagca caagaaaacu acaaacaaga agccaaacau gguuagaauc uagagaauau      420

acuaaacauu uaauacgcgu agaaaacugg auauucagaa auccaggauu ugcacuagca      480

gcagcagcaa uagcaugguu auuaggauca aguacaucac aaaaaguaau auacuuagua      540

augauacuau uaauagcacc agcuuacuca auaagaugca uaggaguauc aaaucgugac      600

uuuguagaag gcaugagcgg gggcacaugg guagauauug uacuagaaca uggugguugu      660

guuacaguua uggcucaaga uaaaccuacc guugauauag aguuaguaac aacaacagua      720

agcaauaugg cagaaguaag aucauacugu uacgaagcau caauaagcga uauggcauca      780

gauaguagau gcccaacuca aggagaagca uacuuagaua aacaaucaga uacacaauau      840

guaugcaagc gaacccuagu ggaccgagga uggggaaaug gauguggacu auuuggaaaa      900

ggcucucuug uuacaugugc aaaauuugca uguucuaaga aaaugacagg aaaaucaaua      960

caaccugaaa acuuagaaua cagaauaaug uuaagcguuc auggaaguca acacucaggu     1020

augauaguaa augauacagg acacgagacc gaugaaaaca gagcaaaagu agaaauaaca     1080

cccaacucac caagagcaga agcaacccuu ggcgguuuug gaaguuuagg auuagauugu     1140

gagccuagaa cugguuuaga uuucucagau uuauauuauu uaacuaugaa uaauaaacau     1200

uggcuagucc auaaagaaug guuucaugau auaccuuugc cauggcaugc gggugcagau     1260

accggaacac cacacuggaa caacaaagaa gcacuuguag aauuuaaaga ugcccacgca     1320

aaaagacaaa cuguaguagu acuuggaagu caagaaggag caguucauac ugcauuagca     1380

ggagcauuag aagcagaaau ggauggagcc aaaggaagau uaaguucugg acaucuuaaa     1440

uguagguuaa aaauggauaa auuaagacua aaaggcguuu cauacucauu auguacagca     1500

gcauucaccu ucacaaaaau accagcugaa acauuacacg gaacaguaac aguugaaguu     1560

caauaugcag gaacugaugg gccauguaaa guaccugcac aaauggcagu agauaugcaa     1620

acacuaacac caguaggaag acuaauaaca gcaaacccug uaauuacgga aucuacagaa     1680

aacagcaaaa ugauguuaga guuggaucca cccuuuggag auagcuauau aguuauagga     1740

gugggugaaa agaaaauaac gcaucauugg cacagaagug guaguacaau aggaaaagca     1800

uuugaggcaa ccguaagagg ggcaaaaagg auggcagucc uaggagacac agcuugggac     1860

uuuggaagcg uaggcggggc ccuuaauucc uuaggaaagg gaauacacca aauauuugga     1920

gcagcauuua aaucauuauu cgguggcaug ucuugguuuu cucaaauacu aauaggaaca     1980

uuauuaaugu ggcuuggacu aaauaccaaa aauggcucaa ucucauuaau gugucuugcu     2040

uuaggaggug uacuaauauu uuuaaguaca gcaguaagug ca                        2082


<210>  9930
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9930
auggguaaaa gaucagcugg aucaauaaug ugguuagccu cacuagcagu uguuauagca       60

ugugcuggag cgguaacuag aagagguagu gccuauuaca uguaccuaga uagaaacgau      120

gcaggagaag ccauaucauu uccaacaaca uuagguauga auaaaugcua uauucaaaua      180

auggauuuag gacacacaug ugaugcaaca augucuuaug aauguccaau guuagaugaa      240

ggaguugaac cagaugaugu agauuguugg uguaauacaa caaguacaug gguuguauac      300

ggaacguguc aucauaaaaa aggagaagca cgaagaagua gacgagcugu cacauuacca      360

agucauagca cucguaaauu acaaacaaga agucaaacuu ggcuagaaag cagagaauau      420

acgaagcauc uaauaagggu ugaaaacugg auauucagaa acccaggauu ugcuuuagca      480

gcugcugcaa uagcauggcu auuaggaagc ucaacaaguc aaaaagucau auauuuagua      540

augauacuuc uaauagcacc agcauacagu auuagaugca uaggaguaag uaauagggau      600

uuuguagaag gaaugagugg agguacaugg guugacguag uauuagaaca uggaggaugu      660

guaacaguaa uggcacaaga caaaccuaca guagacauag aauuaguaac aacuacaguu      720

ucuaauaugg ccgaaguacg aagcuauugu uaugaggcau caauaucaga uauggcauca      780

gacucaagau guccaacuca aggggaagcg uaucuagaua aacaauccga cacccaauac      840

guauguaaac gaacacuugu agauagagga uggggaaacg guuguggauu auuugguaaa      900

gguaguuuag uaacuugcgc aaaauuugca uguucaaaaa agaugacagg caaaucaaua      960

caaccagaaa accuggaaua uagaauaaug uuaucaguac auggcucaca acauaguggu     1020

augaucguua acgauacugg acaugaaaca gaugaaaauc gagcgaaagu agaaauaacg     1080

ccaaauucac cuagagcuga agcaacauua ggaggauuug ggagcuuagg acuugacugu     1140

gaaccuagaa caggauuaga uuucagcgau cuauacuauc uuacgaugaa uaauaaacau     1200

uggcucguac acaaagagug guuucaugau auuccucuuc cauggcacgc aggagcugau     1260

acugggacac cacauuggaa uaauaaagaa gcuuuaguag aauucaaaga ugcacacgca     1320

aaaagacaaa caguaguugu guuaggaucu caagaaggag cgguacauac agcauuagca     1380

ggagcccuag aagcagagau ggauggagca aaaggacggu uaucaucagg acaucuaaaa     1440

uguagacuua aaauggacaa auuaagacua aaaggaguau cuuauucauu augcacagca     1500

gcauuuaccu uuacaaaaau accggcagaa acacuucaug gaacaguuac aguagaagua     1560

caauacgcag gaacagaugg cccaugcaaa gucccagcac aaauggcugu agauaugcaa     1620

acauuaacuc caguagguag acuuauaaca gcaaauccgg uaauaacaga gucaacggaa     1680

aauucaaaaa ugauguuaga acuagaucca ccauuuggag acaguuacau aguuauuggc     1740

gucggagaaa aaaaaauaac acaucacugg cauaggagcg gaaguacaau uggaaaagca     1800

uucgaagcua caguuagagg agccaaaaga auggcuguau uaggagacac ugcgugggac     1860

uuuggaagug uaggaggcgc auuaaauagc uuagguaaag gaauacauca aauauuuggu     1920

gcggcauuca aaucacuguu cggaggaaug aguugguuuu cacaaauacu aaucggaacu     1980

uuacuaaugu gguuagguuu aaaugcaaaa aauggaucaa uaaguuuaau gugccuggca     2040

uuaggaggag uauugauauu uuuaagcaca gcagugucag ca                        2082


<210>  9931
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9931
augggaaaaa gaucagcagg gaguauaaug ugguuagcaa gucuagcagu ugucauagcg       60

ugcgcaggug caaucacuag aagaggaagc gcauauuaca uguauuuaga uagaagugau      120

gcaggaaaag caauuucauu ugcuacaaca uuaggcguca auaaauguca uguacaaauc      180

auggauuuag gucauaugug ugaugcaacc augagcuacg aauguccaau guuagaugaa      240

ggaguagaac cugaugacgu agacugcugg uguaauacaa caaguacaug gguaguauau      300

ggaacgugcc aucacaaaaa aggagaagca agaagaagcc gaagagcggu cacccuacca      360

agucauagca cuagaaaauu acaaacaagg agucaaacau ggcuagaaag cagagaauau      420

acaaaacacu uaauaaaagu agaaaauugg auauuucgua auccaggcuu cgcacuagua      480

gcaguagcca uagcaugguu gcuuggauca ucgacaaguc aaaaaguuau cuauuuagua      540

augauauuau uaauagcgcc ugcauacuca auacgaugua uaggaguauc uaacagagau      600

uucguagaag gaaugucugg agguacuugg guagauguag uucuggaaca ugguggaugu      660

guuacuguaa uggcccaaga caaaccaacc guagauauug aacuaguaac uacuacugua      720

agcaacaugg cggaaguacg aucauauugu uacgaagcaa guauaaguga uauggcauca      780

gauucuagau gcccgaccca gggagaagca uauuuagaua agcaaaguga cacccaauac      840

guauguaaaa gaacauuggu agauagagga uggggaaacg gauguggauu auucggcaaa      900

ggaaguuuag uaacaugugc uaaauuuacc ugcaguaaaa aaaugacagg caaaaguaua      960

caaccagaaa auuuagaaua cagaauaaug cuaagcguac auggaucuca acauucagga     1020

augauaggau augaaacaga ugaagauagg gcuaaaguag aagucacacc uaauagccca     1080

agagcggaag cgacccuggg uggguuugga ucauuaggau uagauugcga accgagaacc     1140

ggauuagauu uuagugauuu auauuauuua acaaugaaca auaaacauug guuagugcau     1200

aaagaguggu uccaugacau accacuacca uggcaugcag gugcagacac uggaacacca     1260

cauuggaaua auaaagaagc auuagucgaa uucaaagaug cucaugcgaa aagacaaaca     1320

guagugguuc uaggaucuca agaaggagca guacacacag cauuagcagg agcgcuagaa     1380

gcagaaaugg auggcgcaaa aggaagauua uucagcgguc auuuaaaaug uagauuaaaa     1440

auggauaaau uaagacuuaa aggaguauca uauucucuau gcacagcagc auuuacuuuu     1500

acuaaaguac cagccgaaac auuacauggc acgguaacug ucgagguaca auaugcaggu     1560

acagacggac cuuguaaaau accaguacaa auggcagugg auaugcaaac auuaacucca     1620

guaggcaggc uaauaacagc uaauccagua auaacagaau caacugaaaa cagcaaaaug     1680

auguuggaau uagauccacc auuuggcgau uccuauauag uaauaggggu aggagauaaa     1740

aaaauaacac accauuggca caggagugga aguacaauag ggaaagcauu ugaagcgaca     1800

guaagaggag caaaaaggau ggcaguacua ggagauacag caugggauuu ugguagcgug     1860

ggagguguau uuaacucacu aggcaagggg auacaucaaa uauuuggcgc agcauuuaaa     1920

agcuuauuug gaggaaugag uugguuuuca caaauucuga uaggaacucu uuugguaugg     1980

uuaggauuaa auacaaaaaa cgguucuaua aguuuaacau gucuagcauu agggggugua     2040

augauauuuc uaaguacugc aguaagcgca                                      2070


<210>  9932
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9932
augggaaaaa gaucagcagg uucaauaaug ugguuagcau cauuagcggu aguaauagca       60

ugugcaggag ccguaacgcg uagagguuca gcuuauuaua uguaucuuga uagaaacgau      120

gcuggugaag caauaucauu uccaaccacc uuaggaauga auaaauguua uauacaaaua      180

auggaccuag gacauaugug ugacgcgaca augucauacg agugcccuau guuagacgaa      240

ggaguagaac cagaugacgu agauuguugg uguaauacaa cgucaacgug gguaguauau      300

ggcacaugcc accauaagaa aggugaagcu cgcagaagca ggagagcagu uacauugcca      360

agccauucga cuagaaaauu gcaaaccaga agucaaacau gguuagaaag uagagaauau      420

acuaaacauc ugauacgagu agagaacugg auauuuagaa auccaggauu cgcguuagca      480

gcggcagcaa uagccugguu auuaggaagu ucaacaaguc agaaaguaau auaucuagua      540

augauacuau uaauugcucc ugcauauuca auaagaugua uaggaguauc aaauagagau      600

uuuguagaag gaaugucugg cggaacuugg guagauguag uacuagaaca cggggguugu      660

gucacuguaa uggcacaaga uaaaccaaca guggauauag aauuaguaac cacaacagua      720

aguaauaugg cagaaguaag aucauauugu uaugaagcaa guauaaguga cauggcauca      780

gauagcagau guccaacaca aggcgaagca uacuuagaua aacaauccga cacacaauac      840

guauguaaaa gaacauuagu cgauagagga uggggaaaug gauguggauu auuuggaaaa      900

gggucauugg uaacaugugc aaaauucgca uguagcaaga aaaugacugg aaaaucaaua      960

caaccagaaa auuuggaaua ucgaauaaug uuaaguguac auggcucuca acauucaggg     1020

augauuguaa acgacacugg acaugaaaca gaugaaaaca gagcaaaagu agaaauaacc     1080

ccgaauaguc cuagagcaga agcaacacua gguggauuug ggucauuagg uuuggauugu     1140

gaaccaagaa caggcuuaga uuuuagugac cuauauuacc ugacuaugaa uaauaaacau     1200

uggcuaguac auaaagaaug guuucaugau auaccauuac cauggcacgc aggagcugau     1260

accggaacuc cacauuggaa uaacaaagaa gcauugguug aauuuaaaga ugcucacgca     1320

aaaagacaaa ccguaguagu acuaggcuca caagaaggag cgguccauac agcacuagca     1380

ggagcacuag aagcagaaau ggacggggca aaaggaaggu uaucaagugg acauuuaaag     1440

uguagacuaa aaauggacaa auuacgauua aaaggaguau cauauucauu auguacagca     1500

gcauuuacuu uuacuaagau cccagcagaa acauugcacg gaacaguaac uguagaaguu     1560

caauaugcag gaacugaugg accaugcaaa guaccugcuc aaauggcugu ugacaugcaa     1620

accuuaaccc ccguaggacg auuaauaaca gcaaauccgg uuauaacaga aucaacugaa     1680

aauaguaaaa ugauguuaga acuagaucca ccauuuggug auaguuacau aguuauagga     1740

guaggagaaa agaaaauaac acaucauugg cauaggagcg gaucaacaau agggaaagca     1800

uuugaagcaa caguaagagg agccaaaaga auggcaguau uaggagauac ggcgugggau     1860

uuuggaagcg uuggaggcgc auuaaauucc cuaggaaaag gaauacauca aauauucgga     1920

gcagcauuua aaucauuauu ugguggaaug ucuugguuua gucaaauacu aauaggaaca     1980

uuauuaaugu gguuaggauu aaauacuaaa aacggaucaa uaagccugau gugcuuagca     2040

cuagguggag uacuaaucuu uuugaguaca gcagugucag cc                        2082


<210>  9933
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9933
auggcaauaa gcggaguacc aguauuagga uuuuuuauaa uagcaguuuu aauguccgcu       60

caagagagcu gggccguuac uagaagagga agugcauauu auauguaucu cgauagaaau      120

gacgcaggag aagcgauauc auuuccaaca acauuaggaa ugaauaaaug cuauauacaa      180

auuauggauc uaggacauau gugugaugca acaaugaguu acgaaugucc aauguuagau      240

gaaggcguag aaccugauga uguagacugu uggugcaaca caacuucaac auggguagua      300

uacggaacgu gucaucacaa aaaaggggaa gcacgaagau caagaagggc aguaaccuua      360

ccaucacacu caacuagaaa auuacaaacu agaagucaaa cauggcuaga aucaagagaa      420

uauacaaaac auuuaauucg aguagaaaac uggauauuca gaaauccagg auuugcacua      480

gcagcagcgg caauagcaug guuacuagga ucaucaacuu cacaaaaggu aaucuaucua      540

guuaugauac uucucauagc accagcuuac aguauuagau gcauuggugu aucuaaucga      600

gauuuuguug aaggaauguc cggaggaacg uggguagaca uaguuuugga acauggagga      660

uguguaacgg ugauggcaca agacaaacca acaguagaua uugaauuagu gacaacaaca      720

guuucaaaua uggcugaagu cagaagcuau uguuaugaag caaguauaag ugauauggcc      780

agugauucaa gaugcccuac acagggggaa gcauaucuug auaaacaauc ugauacucaa      840

uaugucugua aaagaacuuu aguugauaga ggauggggca auggaugugg cuuauucgga      900

aaaggaaguu uaguaacaug ugcuaaauuc gcauguucaa aaaaaaugac aggaaaaagc      960

auacaaccug aaaaucuaga auaucguaua auguuaagug uacacggauc acaacacuca     1020

ggaaugauag uuaaugauac uggacaugaa acagaugaaa acagagcaaa aguagagauu     1080

acaccaaaua guccgcgcgc ugaagcaaca uugggaggau uuggaagccu agguuuagac     1140

ugugaaccaa gaaccggucu agauuuuuca gaccuauacu auuugacaau gaauaacaaa     1200

cauuggcuag uucauaaaga augguuucau gauauaccac uacccuggca cgcaggagca     1260

gauacaggaa caccucauug gaauaauaaa gaagccuuag uagaauucaa agaugcacau     1320

gccaaaagac aaacaguagu aguauuagga ucacaagaag gagcuguuca uacagcacuc     1380

gcaggcgcau uagaagcuga aauggaugga gcuaaaggac gacuaucuuc uggacacuua     1440

aaaugucggu uaaaaaugga uaaauuacga uuaaaaggag uaucauauag ccuauguacu     1500

gcggcauuua ccuucacaaa aauaccagca gaaacacuac auggaacagu cacgguagaa     1560

guacaguaug caggaacaga uggaccaugu aaaguaccag cucaaauggc aguugacaug     1620

caaacacuaa caccaguagg aagacuaaua acugcaaauc cuguaaucac cgaaaguacu     1680

gaaaacagua aaaugaugcu ugaauuagau ccaccguuug gagauagcua uauaguaaua     1740

ggaguuggag aaaaaaaaau aacccaccau uggcacagga guggaucaac auuaggaaaa     1800

gcuuuuucua caacauugaa aggagcgcaa cgucuagcug cauuaggaga cacagcuugg     1860

gauuuuggau caauaggagg aguauuuaau ucaauaggaa gagccgugca ccaaguauuc     1920

ggaggagccu uuagaaccuu auucggcgga augaguugga uaacacaagg acuaauggga     1980

gcucuauuac uauggauggg aguuaaugcc agagauagau ccauugcacu ggccuuuuua     2040

gcgacaggag ggguauuagu auuuuuagca acaaauguac augca                     2085


<210>  9934
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9934
auggcaauaa gcggaguacc aguauuagga uucuuuauaa uagcaguacu aaugucagcu       60

caagaaaguu gggccguaac aagaagagga uccgcauauu auauguaucu agauagaaau      120

gaugcuggug aagcaauauc uuucccuacc acauuaggaa ugaauaaaug uuauauacaa      180

auuauggauc ugggacacac augugaugca acgaugucuu acgagugucc aauguuagac      240

gaagguguug aaccagacga uguugauugu uggugcaaca ccacaucaac uuggguagua      300

uaugguacau gucaucacaa aaaaggcgaa gcgagaagau caaggagagc cguaacauua      360

ccaagucacu caacuaggaa auuacaaaca agaucacaaa ccugguuaga aucaagagaa      420

uauacaaaac auuuaauaag aguagaaaau uggaucuuuc gaaacccagg auuugcuuua      480

gcugcagcag caauagcuug guuauuagga ucaagcacau cacaaaaagu aauauauuua      540

guaaugauau uacuaauagc uccagcauac uccaucagau guauuggugu cuccaauaga      600

gauuucguug aaggaaugag cggaggaacu uggguagaug uaguuuuaga acauggcgga      660

uguguaacug uaauggcaca agauaaacca acaguagaca uagaguuagu uacaacaacc      720

guauccaaua uggcugaagu aagaagcuau ugcuaugaag cuucaauauc ugauauggca      780

agugacucaa gaugcccaac acaaggugaa gcauaucuag acaaacaauc agacacacaa      840

uauguaugua aaaggacacu aguagacaga ggauggggaa augguugugg cuuauuuggg      900

aaagggaguu uagucacaug cgcaaaauuc gcauguucaa aaaaaaugac aggaaaauca      960

auccaaccag aaaaucuaga auaucguaua augcuaucug uucauggauc ucaacauagc     1020

ggaaugauug uaaaugacac aggacacgaa accgaugaaa acagagcaaa aguagaaaua     1080

acaccaaauu ccccaagagc cgaagcaaca cuaggagguu uuggaucuuu aggacucgau     1140

ugugaaccaa gaacaggauu ggauuucagc gaccuuuauu acuuaacaau gaauaauaaa     1200

cacugguuag uacauaaaga gugguuccau gauauuccau uaccuuggca cgcaggagcu     1260

gauacaggaa cuccacacug gaauaauaaa gaagcauuag uagaauucaa agaugcacac     1320

gcaaaacgac aaacuguagu aguauuggga ucacaagaag gagcagugca uaccgcauua     1380

gcuggagcac uagaggcaga aauggaugga gcaaaaggcc gauuaucauc aggacaucuu     1440

aaaugucguc uaaaaaugga uaaauugaga cuaaaaggcg uaucauauuc acuuuguacc     1500

gcugcuuuua cuuuuaccaa aauaccagcc gaaacucuac augguaccgu aacagucgaa     1560

guucaauaug cagggacaga uggaccaugu aaaguuccag cacaaauggc agucgacaug     1620

caaacauuaa cgccgguagg aagguugauu acagcaaauc caguaauuac agaaagcaca     1680

gaaaauucaa aaaugauguu agaauuagau ccacccuuug gagauaguua uauaguaaua     1740

ggaguaggag aaaaaaaaau uacacaccac uggcaccggu caggaucuac ucuaggaaaa     1800

gcuuuuagua cuacauuaaa aggagcacaa agauuagcag cacuaggaga uacagccugg     1860

gauuuuggau cuauaggugg aguguuuaac uccauaggaa gagcuguaca ccaaguuuuu     1920

ggcggugcuu uuagaacucu uuuugguggu augucaugga uaacacaggg acuuaugggg     1980

gcuuuacuac uguggauggg aguaaacgcc agagauagau cuauagcacu agccuuucua     2040

gcaacaggag gaguauuagu cuucuuagca acuaauguac acgca                     2085


<210>  9935
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9935
auggccauaa guggaguacc uguauuggga uucuuuauaa uagcgguauu aaugucagca       60

caagaaucau gggcgauaac aagaagggga agcgccuauu auauguacuu agauagaagc      120

gaugcaggaa aagcaauaag uuuugcaacu acucuaggag uaaauaaaug ccauguucaa      180

auaauggauu uaggacacau gugugaugcu acaaugucuu augaaugucc gauguuagac      240

gaaggagucg aaccugacga uguggauugc ugguguaaca caacuucaac auggguagua      300

uacggaacuu gucaucauaa aaaaggugaa gcgcgaagau cccgaagagc aguaacucuc      360

ccuagucacu caacacgaaa guuacaaaca agaagucaaa cuugguuaga gaguagagaa      420

uauacaaagc acuuaauaaa aguagaaaau uggauuuuua ggaauccagg uuuugcgcuu      480

guagcaguag caaucgcuug guuauuagga ucaucaacau cacaaaaagu aauauacuua      540

guaaugauau uacucauagc accagcauac aguauaagau guauuggggu aaguaaucga      600

gauuuugugg aaggaaugag ugguggaaca uggguggaug uaguacuuga gcacggaggc      660

ugcguaacug uaauggcuca agauaaacca acaguagaua ucgaauuagu aacuacuaca      720

guuucaaaca uggcugaagu aagaucguau uguuaugaag cgagcauauc agauauggcc      780

agugacucaa gaugccccac gcaaggagaa gcauaucuug auaaacaaag ugauacucaa      840

uauguaugua aaagaacauu aguagaccga ggauggggaa auggcugugg auuauucggu      900

aaaggaaguu uaguaacaug cgcaaaauuu acauguucaa aaaaaaugac aggaaaaagc      960

auucaaccgg aaaaccuaga auaucgaauu auguugucgg uucacggaag ucagcauagc     1020

ggaaugauag gauaugaaac agaugaagau agagcaaaag uagaaguaac accaaauagu     1080

ccacgugcag aagcaacucu cggcggguuu ggaagucuag gacuagauug ugaaccaaga     1140

acaggacuag auuuuaguga ucuauacuau uuaacaauga auaauaaaca uugguuaguc     1200

cauaaagaau gguuucauga uauaccacuu ccauggcaug cuggagcaga uacaggcaca     1260

ccacauugga auaauaaaga agcucuagua gaguuuaaag acgcccaugc aaaaagacag     1320

acagugguug uacuagguag ccaagaagga gcuguucaua cagcauuagc aggagcccuu     1380

gaagcagaaa uggauggagc uaaaggaaga uuauuuagug gacauuuaaa augcagacuc     1440

aaaauggaua aacuuagacu aaaaggugua ucuuacaguc uaugcacagc cgcauuuaca     1500

uuuacaaaag ucccagcaga gacacuacac ggaacaguga caguagaagu acaauaugca     1560

ggcacugaug gaccaugcaa aauacccgua caaauggcag uagauaugca aacguuaacc     1620

ccaguaggaa gacuaaucac ugcaaaccca guaauuacag aaaguacuga gaauucaaaa     1680

augaugcuag aauuagaucc accauucgga gacaguuaca uagugauagg aguaggagau     1740

aaaaaaauua cacaucauug gcauagauca gguaguacau uaggaaaagc auuuagcaca     1800

acauuaaaag gagcacaaag auuagcagca uuaggcgaua cagcauggga uuuugggagc     1860

auagguggcg uauucaacuc uauaggcagg gccgugcauc aaguauuugg uggagcauuc     1920

agaacucuau uuggaggcau gucauggauu acacaagguu uaaugggagc auuacuauua     1980

uggaugggag uaaaugcaag agauagaagu auagcauuag cguuuuuagc aacaggagga     2040

guuuuaguau ucuuagcaac uaauguccac gca                                  2073


<210>  9936
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9936
auggcaauuu caggaguacc aguauuagga uucuuuauua uagccguacu aaugucugca       60

caagaaagcu gggcggugac aagaagggga agcgcauacu auauguaucu agacagaaau      120

gaugcaggag aagcaauauc uuuuccuaca acauuaggaa ugaauaaaug uuauauacaa      180

auaauggacu uaggacauau gugugaugca acgaugagcu augaaugccc gaugcucgac      240

gaagguguag aaccagauga uguagauugu ugguguaaua ccacuagcac auggguaguc      300

uauggaacgu gccaucauaa aaagggcgaa gcccgaagau cuagacgagc aguaacguug      360

ccaucucaua guacaagaaa gcugcaaaca agaagucaaa cauggcuaga aucgagagaa      420

uauacaaaac acuuaauuag aguagaaaau uggauauuua gaaauccagg auuugcuuua      480

gcagcugcag caauagccug gcuauuagga ucaucaacaa gucaaaaagu gauauauuua      540

guaaugauac ucuugauagc accagccuau ucaauaagau guauaggugu aucaaacagg      600

gauuuugugg aaggaaugag uggaggaacu uggguagacg ucgucuuaga acauggagga      660

uguguaacag uuauggcaca agauaaacca acaguagaua uagaauuagu cacaacuacu      720

guaucuaaua uggcagaagu aaggucauac ugcuaugaag caucaauuag cgauauggcu      780

agugauagua gaugcccaac ucaaggagaa gcauaucuag acaaacaguc agauacacaa      840

uauguaugca aaagaacacu uguagauaga ggguggggaa acggaugugg acuuuuuggg      900

aaaggcagcu uaguaacaug ugcaaaauuu gcaugcagua agaaaaugac uggaaaaagu      960

auacagccag aaaaccuaga auacagaaua auguuaagug uacacggauc ucaacauagu     1020

ggaaugauag uaaaugauac cggacacgaa acugaugaaa aucgagcuaa aguagaaaua     1080

accccaaaca guccgagagc agaagcaacu uuaggaggau uuggaaguuu aggacuagac     1140

ugugaaccaa gaacaggacu ugacuuuucu gaucucuauu acuuaacaau gaauaauaaa     1200

cacuggcuag uacauaaaga augguuucau gauauaccac uaccauggca ugccggugca     1260

gauacuggca caccucauug gaauaacaaa gaggcuuuag uagaauuuaa agaugcacau     1320

gcaaaaagac aaacaguagu agugcuagga ucacaagaag gugcaguaca caccgcacua     1380

gccggagcau uagaagcuga aauggauggu gcaaaaggaa ggcuaucauc aggacauuua     1440

aaauguaggu uaaaaaugga uaaacuaaga uuaaaaggag uaucauauuc auuauguacu     1500

gcagcauuca cauuuacaaa aauaccggcc gaaacgcuac auggaacugu aacaguugaa     1560

guucaauaug cgggaacaga uggaccgugc aaaguaccag cacaaauggc aguagauaug     1620

caaacucuaa caccaguugg aagauugaua acagcuaauc caguuauaac agaaaguaca     1680

gaaaacagua aaaugaugcu agaauuagau ccaccguuug gagauucuua cauuguuaua     1740

ggaguaggug agaagaaaau uacucaucac uggcauagaa guggcucaac acuaggaaaa     1800

gcauucagca cuacacuuaa aggagcacaa agauuagcag cucuagggga uacagccugg     1860

gacuucggcu caaucggagg agucuucaau aguauaggga gagcaguaca ccaaguauuu     1920

ggaggagcau uuagaacauu auuuggaggu augagcugga uaacucaagg uuuaauggga     1980

gcacuucucc uauggauggg ugucaacgca agagaccgua guauagcuuu agcuuuucua     2040

gcaacaggag gaguauuagu auucuuagca acaaacgucc augcg                     2085


<210>  9937
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9937
auggauugga cauggauauu auuuuuagua gcagcugcaa caagaguaca uaguguaaca       60

aggcgcggaa gcgcuuacua uauguaucug gauagaaacg augcaggaga agcuauauca      120

uuuccuacca cacuuggcau gaauaaaugu uauauccaaa uaauggaucu uggucauaug      180

ugcgacgcaa ccaugaguua cgaaugccca augcuagaug aagguguaga accugaugau      240

gucgauuguu ggugcaacac gacuucgacu uggguaguau augguacaug ucaucacaaa      300

aaaggagaag cuagaagaag uagaagagca gucacauuac caucacauuc aacaaggaaa      360

uuacagacaa gaagucaaac auggcuagaa ucaagagaau auaccaaaca uuuaauaaga      420

guugaaaauu ggauauucag aaauccagga uuugcauuag cagcugccgc cauagcaugg      480

uuauuaggga guucaacauc acaaaaagua auuuacuuag uuaugauucu uuuaauagca      540

ccugcauaua gcauuagaug uauuggagua aguaauagag auuuugugga aggaaugucu      600

gguggaacau ggguggauau cguacuggaa cauggaggau guguaacugu aauggcgcaa      660

gauaaaccaa cuguagacau agaauuaguu acaacuacag uaucaaauau ggccgaaguu      720

agaaguuauu guuaugaagc aucaauaagu gauauggcau cagauaguag guguccaaca      780

caaggagaag cuuauuugga uaaacaaucc gacacacaau acguaugcaa aaggacucua      840

guugauagag gauggggaaa uggaugcgga uuauucggua aaggaucauu aguuaccugu      900

gcuaaauucg cguguucuaa aaaaaugaca gggaaaagua uucaaccaga aaaccuagaa      960

uacagaauua ugcuuagugu gcauggaagc caacauucag gaaugauugu caacgacaca     1020

ggucaugaaa cagaugaaaa ucgagcaaaa guagaaauaa caccuaauuc accacgugca     1080

gaagcaacau uaggaggauu uggaucauua ggauuagauu gugaaccaag gacaggauua     1140

gauuucagcg acuuauacua ucugaccaug aauaauaaac acugguuagu acacaaagaa     1200

ugguuccaug auauaccauu accauggcac gcaggagcag acacaggaac accacacugg     1260

aauaauaaag aggcccuugu ggaauucaaa gaugcacaug caaaacgaca aacaguagua     1320

guacuuggau cacaagaggg agcaguucau acagcauuag caggagcacu ugaggcagaa     1380

auggacggug caaaaggaag auuaaguagu ggacauuuaa aauguagacu aaaaauggac     1440

aaacuaagau uaaaaggagu uagcuauagu uuauguacag cagcauucac auucacaaaa     1500

auuccagcag aaacauuaca cggaacaguc acaguagaag uacaauaugc aggaacagac     1560

ggaccaugua aaguuccggc acaaauggca guagauaugc aaacauuaac accaguugga     1620

cguuuaauaa cagcuaaucc aguaauaacu gaaagcacgg aaaauucuaa aaugaugcua     1680

gaacucgauc caccuuuugg agauucuuac aucguaauag gaguggggga aaaaaaaauu     1740

acucaccacu ggcauagaag uggaucaaca uuagguaaag cauucaguac aaccuuaaaa     1800

ggagcacaac gacuagcagc auuaggcgac acagcauggg auuuugguuc gauaggagga     1860

guguuuaauu caauaggacg cgcaguucau caaguuuuug gaggagcauu uagaacauua     1920

uuuggaggaa ugucauggau aacacaggga cuuaugggag cccuauuauu auggauggga     1980

gugaaugcua gagauagauc aauagcucua gcauucuuag caacaggugg uguauuagug     2040

uuucuagcaa caaauguaca cgcu                                            2064


<210>  9938
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9938
auggacugga cuuggauacu cuuuuuagua gcugcagcaa caagaguaca cucaguaaca       60

cgaaggggau ccgcauauua uauguauuua gauagaaacg augcaggcga agcaauuuca      120

uuuccaacaa cauuaggcau gaauaaaugc uauauucaaa uaauggaccu agggcauaca      180

ugcgaugcca cuaugagcua ugaaugucca auguuagaug aaggagugga accagaugau      240

guagacugcu gguguaauac aacaaguaca uggguuguau auggcacaug ccaucauaaa      300

aaaggggaag caagaagauc cagaagagcu guaacauuac cuagccauag uacaagaaaa      360

uuacaaacaa gaagccaaac auggcuagaa ucaagagagu auacaaaaca ccuaauaaga      420

guagaaaauu ggauauuuag aaacccaggu uucgcauugg cagcagcagc aauugcgugg      480

uuacuaggau caucaacauc acaaaaagua auauaucuag uaaugauauu auuaauagca      540

ccagcuuauu caauaagaug uauaggcgua aguaauagag auuuuguaga aggaaugagc      600

ggaggaacau ggguggaugu aguauuagag cauggaggau guguaaccgu aauggcacaa      660

gauaaaccaa caguugauau agaguugguu acuacaacug uuucaaauau ggcagaggua      720

cguucuuacu gcuaugaagc aaguaucagc gauauggcaa gugacucacg uugcccaacu      780

cagggggagg ccuauuuaga caaacaaucc gauacacaau auguuuguaa aagaacacua      840

guagacagag gauggggaaa cggauguggu uuauuuggaa aaggcaguuu agucacuugu      900

gcaaaguucg cauguaguaa aaaaaugaca gguaaaagca uccaaccaga gaaccuagaa      960

uacagaauaa uguuaagugu ucacggaucu caacauagcg gaaugauugu gaacgauacu     1020

ggucaugaaa cggaugaaaa uagagcuaaa guagaaauua caccaaauag ucccagagca     1080

gaagcaaccc uaggcggauu cggaucauug ggacuugacu gcgaaccuag aaccggacuu     1140

gauuuuucug acuuguacua cuuaacaaug aauaauaaac acugguuagu ucauaaagaa     1200

ugguuccaug auauaccacu gccauggcac gcaggagcag auacugguac accucauugg     1260

aauaauaaag aagcauuagu ugaauucaaa gacgcacaug caaaaagaca aacaguagua     1320

guauuaggaa gccaagaagg agcaguacac acagcacuag caggagcuuu ggaagcagaa     1380

auggacggug cuaaaggaag auugagcagu ggacaucuaa aaugucgacu uaaaauggau     1440

aaauuaagau uaaaaggugu aagcuauuca cuauguacug cagcauucac auuuacgaaa     1500

auaccagcag aaacucuaca cgguacaguu acaguagaag uccaauaugc aggaacagau     1560

ggaccaugua aaguucccgc acaaauggca guggacaugc aaacauuaac uccgguugga     1620

agauuaauaa cugcaaaucc agugauuacg gaaucgacag aaaauucaaa aaugaugcuu     1680

gaauuagacc cuccuuuugg agauucauac auaguaaucg guguagguga aaaaaaaauu     1740

acacaucauu ggcaucgguc agguucaacu uuagggaaag cauuuucgac aacacuaaaa     1800

ggcgcacaaa gauuagcagc acuaggcgac acagcauggg auuucggaag uauaggagga     1860

guuuuuaaua gcauaggaag agccguacau caaguauuug gaggagcuuu ucggacucua     1920

uuuggaggca ugucauggau aacacaaggu uuaauggggg cauuauuauu auggauggga     1980

guuaaugcac gugauagaag cauagcguua gcauuuuuag caacuggagg aguauuagua     2040

uucuuagcaa caaacguaca ugca                                            2064


<210>  9939
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9939
auggacugga cauggauacu auuucuagua gccgcagcga cuagaguaca uucaauuaca       60

agaagaggaa gcgcauauua uauguauuua gauaggucag augcugggaa agcaauauca      120

uucgcaacua cuuugggugu aaacaaaugc cacguacaaa uaauggaucu aggccauaug      180

ugugaugcaa caaugucaua ugaaugucca auguuagacg aagguguaga accagaugau      240

guugauugcu gguguaacac aaccucaacc ugggugguuu augguacuug ucaccacaag      300

aaaggugaag cuagaaggag uaggagggcc guaacacuuc caucacauag cacaagaaaa      360

cuucaaacua gaucacaaac augguuagaa ucaagggaau acacaaaaca ucuaaucaaa      420

gucgaaaauu ggauauuuag aaacccagga uuugcucuag uugcaguagc cauagccugg      480

cucuuaggau caagcacuuc acagaaaguu auauauuuag uuaugauacu auuaauagca      540

cccgcuuauu ccauaagaug uauaggagug aguaaucgag acuuuguaga aggaauguca      600

ggugguacgu ggguggaugu aguauuagaa cauggaggau guguaacagu uauggcacaa      660

gacaaaccaa caguagauau agaauuaguc acaacaacag uaucaaauau ggcagaaguu      720

agaucauauu gcuacgaagc gucaauaagu gauauggccu cagauaguag auguccuaca      780

caaggagaag cguauuuaga caaacaauca gacacacaau auguuuguaa aaggacucua      840

guugauagag gaugggguaa uggaugugga uuauucggaa aaggaucccu aguaacaugu      900

gccaaauuua cauguaguaa gaaaaugacc ggaaaaucua uacagccaga aaauuuagaa      960

uaucguauaa uguuaagcgu acaugguagu caacacagcg guaugauagg guaugaaacc     1020

gacgaagaca gagcaaaagu agaaguaaca ccaaacucac caagagcuga agccaccuua     1080

ggaggauucg gaagucuugg auuagauugu gaaccaagaa cuggauuaga uuucucagac     1140

cuuuauuauu uaacuaugaa uaauaaacau ugguuaguac auaaagaaug guuucacgac     1200

auaccuuuac cauggcaugc aggagcagau acaggaacgc cacacuggaa caauaaagaa     1260

gcauuaguag aauuuaaaga ugcucacgcu aaaagacaaa caguuguagu auuaggguca     1320

caagaaggug cuguacauac ugcauuagca ggagcacuag aagccgaaau ggacggagca     1380

aaaggcagau uauuuagugg acaucuuaaa ugcagauuaa aaauggauaa acuaagacua     1440

aaaggaguaa gcuacagccu auguacugcc gcauuuacau uuacaaaagu uccagcugaa     1500

acacuacaug ggacaguaac aguagaaguc caauacgcag gaacugaugg accauguaaa     1560

auaccaguac aaauggcagu agacaugcaa acacuuacac caguugggag guuaauaaca     1620

gcuaaucccg uaaucacuga aucaacagaa aauaguaaaa ugauguuaga acuugaccca     1680

ccauuuggag auucauacau uguaauugga guaggugaua agaaaauaac acaucauugg     1740

cauagaucug gaucaacacu uggaaaagca uucagcacaa cauugaaagg agcacaaaga     1800

uuagcggcau ugggggauac agcaugggau uucggaagua uaggaggagu auuuaauuca     1860

auuggucgag caguccacca aguauuugga ggagccuuua gaacauuauu cggaggaaug     1920

ucauggauua cacaaggauu aaugggagcc cuauugcuuu ggaugggagu uaaugcacga     1980

gaccguucga uagcucucgc auuucucgcu acuggcggag uucuaguauu uuuagcaacg     2040

aauguucaug cu                                                         2052


<210>  9940
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9940
auggauugga cauggauucu auuucuugua gcagccgcaa caaggguaca uagugucaca       60

agaaggggaa gugcauauua uauguaucua gacagaaaug augcugguga agccauaagu      120

uuuccaacaa cgcuaggaau gaauaaaugu uacauccaaa uaauggaucu cggccauaug      180

ugugaugcga caaugaguua ugaaugccca augcucgaug aagggguaga accagaugau      240

guagauuguu gguguaauac aacaucaacu uggguaguuu auggaacaug ucaucauaaa      300

aaaggugaag cuagaagauc gaggagagcu guaacuuuac cuucucauag cacaagaaaa      360

uuacaaacaa gaagccaaac uugguuagaa agcagagaau acacaaaaca uuuaauuaga      420

guagaaaauu ggauauuucg aaauccagga uucgcauuag cugcagcagc aauagcaugg      480

cuucuaggcu ccaguacauc ucaaaaagug aucuauuuag uaaugauacu ucuaaucgca      540

ccagcauaca guaucagaug caucggugua ucaaauagag auuuuguuga aggaaugucg      600

ggcggaacau ggguagacgu cguacucgag cauggcggcu guguaacagu aauggcacaa      660

gacaaaccaa caguugauau agaauuaguu acaacuacgg uaaguaauau ggcagaaguu      720

aggagcuacu guuaugaagc aucaauauca gauauggcau cagauucaag gugcccaacg      780

caaggagaag cauauuuaga uaaacaaagu gacacucaau acguauguaa aagaacauua      840

guugauagag gauggggaaa cggauguggu uuauuuggaa aaggaagcuu aguaacaugu      900

gcaaaauuug ccuguucaaa aaaaaugaca ggaaaaagua uacaaccaga aaaucuagaa      960

uaccguauaa ugcuaucagu acauggaagu caacacagcg ggaugauagu aaacgacaca     1020

ggacacgaaa cagaugaaaa ucgugcaaag gucgaaauua ccccaaacag cccaagagca     1080

gaagcaacuc uuggagguuu cggaucauua ggacuagauu gcgagccaag aaccgguuua     1140

gauuuuagcg auuuauacua uuuaacaaug aauaauaaac auugguuagu ucauaaagaa     1200

ugguuucaug auauaccacu accauggcau gcaggugcgg auacuggaac accucauugg     1260

aacaauaaag aagcacuagu agaauucaaa gacgcacaug cuaaaagaca aacaguuguc     1320

guauuaggaa gucaagaagg agccguacau acugcacuag caggugcguu agaagccgag     1380

auggauggag caaaaggccg acuuaguagc ggucauuuaa aaugcagauu aaaaauggau     1440

aaauugagac ugaaaggugu gucauauuca cuauguacug cagcauuuac auucacaaaa     1500

auaccugcag aaacacuaca cggaacuguc acaguagaag uacaauacgc agguacagau     1560

gguccaugua aaguaccagc ucaaauggcu guagacaugc aaacauuaac accaguugga     1620

agacuaauaa cagcaaaucc cguaauaacu gaaagcacag aaaauucaaa aaugauguug     1680

gaacuugauc caccauuugg cgauaguuac auaguaauag gaguuggaga aaaaaaaaua     1740

acacaucauu ggcacagaag uggaagcacc uuaggaaaag cuuuuucaac aacauuaaaa     1800

ggagcacaaa gauuagcagc auuaggugac acagcauggg auuuuggcuc aauugguggu     1860

guauucaaua guauagggag agcaguacau caaguuuucg gcggagcauu uagaaccuua     1920

uuuggcggaa ugaguuggau cacucaagga cuaaugggag cacuauuacu cuggaugggu     1980

guaaaugcaa gagauagaag cauagccuua gcauuucuug cuacaggagg aguauuagua     2040

uuccuagcua ccaauguaca ugcu                                            2064


<210>  9941
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9941
augggcaaac guagugcagg aucgauaaug uggcuagccu cauuagcagu aguaauagca       60

ugugccggag caguaacgag gcgcggauca gcuuauuaua uguaccuaga cagaaaugau      120

gcaggagaag caauaucauu cccuacaaca cuuggaauga auaaauguua uauacaaaua      180

auggauuuag gacauaugug cgacgcaacu augaguuacg aaugcccaau gcuugaugaa      240

ggcguagaac cagaugaugu ugauugcugg ugcaacacaa cgucaaccug gguaguguau      300

gggacauguc aucacaagaa aggagaagcu agaagaagua gacgagcagu aacguuaccg      360

agucauagua cgagaaaauu acaaacacga ucacaaacau gguuagaaag cagagaguac      420

acaaaacauu uaaucagagu cgaaaauugg auuuucagga auccuggguu ugcauuagca      480

gcagcggcaa uagcaugguu auuaggauca aguacaucac aaaaaguaau cuaucuagua      540

augauucugu uaaucgcucc cgcauauucc auuagaugua uaggaguauc aaacagagau      600

uuuguggaag gaauguccgg aggaacaugg guagauauag uuuuggaaca uggagggugu      660

guaacaguca uggcacagga uaaaccaacg guggauauag agcuaguaac aacaacagua      720

ucaaauaugg cagaaguaag aucuuauugu uaugaagcau cuauaaguga uauggccagu      780

gacaguagau guccaacaca aggggaagca uaucuagaca aacaaaguga uacacaauac      840

guauguaaaa gaacacuugu agauagagga uggggaaaug gaugcggauu auucggaaaa      900

ggaucuuuag uaacaugcgc aaaauucgca ugcucaaaga aaaugacagg uaaaaguauu      960

caaccagaaa aucucgaaua uagaauuaug uuaaguguuc auggaucaca acacaguggu     1020

augauaguaa acgauacagg gcaugaaacc gaugaaaaca gagcaaaagu agaaauaaca     1080

ccuaauucac cuagagcaga agcuacucug ggaggauucg ggagucuugg acuagauugc     1140

gaaccaagaa caggucuuga uuuuucugau cuauauuauc ucacaaugaa uaauaaacau     1200

ugguuaguuc acaaagaaug guuucacgac auaccguuac cauggcaugc cggagcagau     1260

acgggaacuc cacauuggaa uaauaaagaa gcucuuguag aauuuaaaga ugcucaugca     1320

aaaaggcaaa caguaguagu uuuaggcagc caagaaggag caguacacac agcuuuagcu     1380

ggagcauuag aagcagaaau ggauggagcg aaaggaagac uaucgucagg ucauuugaaa     1440

ugcagacuua aaauggacaa auuaagacua aaaggaguuu cauauucauu auguacugca     1500

gcauucacuu uuacaaaaau accagcagaa acacuacaug gcacaguaac uguagaagua     1560

caauaugcug gaacagacgg accaugcaaa gucccugccc aaauggcagu ggauaugcaa     1620

acacuaacac cagugggaag acuaauaaca gcaaauccag uaauaacaga aucaacagaa     1680

aacucaaaaa ugauguugga auuagaucca ccuuuuggag acaguuauau uguaauaggc     1740

guaggagaaa agaaaauuac acaccauugg cauagaagug gaagcacacu uggaaaagcc     1800

uuuagcacaa cauuaaaagg agcucaaaga uuagcugcac uaggagauac agcgugggau     1860

uuuggaagua uaggaggugu cuuuaauagu aucggcagag caguacauca aguauucgga     1920

ggagcauuca gaacacuauu uggcggcaug ucauggauua cacaagguuu aaugggagcu     1980

cuauuauuau ggaugggugu aaacgcuagg gaucggagua uugccuuagc uuuucuagca     2040

acaggaggug uguuaguauu uuuagcaacg aacgugcacg ca                        2082


<210>  9942
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9942
augggaaaac gaucagcagg aucuauaaug uggcucgcau cauuagccgu aguuauagca       60

ugugcuggag caguaacaag acgagguagc gcauacuaua uguaucugga uagaaaugau      120

gcgggagaag cuauaucguu uccaacaaca cuagguauga auaaauguua uauacaaaua      180

auggauuuag gacauacaug ugaugcaaca augucauaug aauguccaau gcuagacgaa      240

ggaguugaac cagacgacgu agacugcugg uguaauacaa caaguacuug gguagucuau      300

ggaacgugcc aucauaaaaa aggugaagca agaagguccc gaagggcagu uaccuuaccc      360

agucauucaa cuagaaaacu ucagacaaga ucacaaaccu ggcuagaauc acgagaguau      420

acaaaacauu uaauaagagu cgaaaacugg auauuuagaa auccuggauu cgcacuagcc      480

gcagcugcaa uagcauggcu ccuaggaucc uccacaaguc aaaaaguaau auauuuagua      540

augauccuac ucauugcacc agcauauucu auaaggugua ucggaguaag caaucgagac      600

uuuguugagg gaaugagugg cgguacaugg guagauguug uacuagaaca cggcggcugc      660

guaacaguaa uggcucaaga uaaaccuaca guagauauag aauuaguaac uacuacagua      720

ucuaacaugg cagaaguaag aucuuacugu uaugaagccu caauaagcga uauggccuca      780

gauucaagau guccaacaca aggagaagca uacuuagaua aacaaucuga uacacaauac      840

guaugcaaga gaacacuagu agauagagga ugggguaaug guuguggauu auucggaaaa      900

ggaagccuag uaacuugugc aaaauuugca uguaguaaaa aaaugacggg aaaaaguauc      960

caaccugaaa auuuagaaua uagaauaaug cuaucaguuc auggcaguca acauagcgga     1020

augauuguaa augauacagg ucaugaaacc gacgaaaaca gagcaaaagu agaaauuacc     1080

ccaaauaguc cuagagcaga agccacuuua ggaggcuuug gaucccuagg ucuugauugu     1140

gaaccaagaa caggauuaga uuuuucggac uuauauuacc uuacaaugaa uaauaaacac     1200

uggcuaguuc auaaagaaug guuccaugac auaccuuuac cuuggcaugc aggagcagac     1260

acuggcacac cacacuggaa caauaaagaa gcgcucguag aauucaaaga cgcccaugca     1320

aaaagacaaa cuguuguagu auugggauca caagaaggcg cuguacauac agcauuagca     1380

ggagcucuug aagcagaaau ggauggagcg aaaggaagac uaucaucggg gcacuuaaaa     1440

ugcagauuaa aaauggauaa guuacguuua aaagggguua gcuacucucu uugcacagca     1500

gcauuuaccu uuacaaaaau accagccgaa acacuacacg guaccguaac aguagaagug     1560

caauaugcug gaacugaugg uccauguaaa guaccugcac agauggcugu agauaugcaa     1620

acacuaacgc caguaggaag auuaauaaca gcaaauccag uaauaacuga aucaacagaa     1680

aacucaaaaa ugauguugga acuagaucca ccauuuggag auaguuauau aguaauagga     1740

guaggagaaa aaaaaauaac ucaccacugg cauagaucag guaguacguu agguaaagca     1800

uuuucgacaa cauuaaaagg agcacagaga uuagcugcac uuggagauac ggcuugggau     1860

uucggaucaa uaggaggagu auuuaauagu auuggaagag cgguacauca aguuuuuggu     1920

ggagcauuua gaacccuauu uggaggaaug ucuuggauua cacaaggacu uaugggagca     1980

uuauuauuau ggaugggagu aaacgcucga gacagaucaa ucgcauuagc auuuuuagcg     2040

acaggaggag uacuaguauu ucucgcaaca aacguacacg cu                        2082


<210>  9943
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9943
augggaaaaa gaagugcagg aucaauaaug ugguuagcau cacuggcggu aguuauagca       60

ugcgcaggag cuauaacaag gaggggauca gcauacuaua uguauuuaga caggagugau      120

gcaggaaaag caaucucauu ugcaaccaca cuagguguaa auaaauguca uguccaaaua      180

auggaccuag gucauaugug ugaugcuaca augucguaug aaugcccaau gcuugacgaa      240

gguguggaac cagaugacgu agauugcugg ugcaacacaa caagcacaug gguaguuuau      300

gggacauguc accauaagaa aggugaagca cgaagaucac ggcgagcagu aacuuuacca      360

ucacauucaa caaggaaacu acaaacaaga ucacaaacau gguuagaauc aagagaguau      420

acaaaacauc uaauuaaagu cgaaaauugg auauuuagaa auccuggauu ugcccuaguu      480

gcaguugcaa uagcuuggcu ucuuggaagc ucaacauccc aaaaaguaau uuauuuagua      540

augauacuac ugaucgcucc ugcauacagu auaagaugua uaggaguaag uaauagagac      600

uucguggaag gaauguccgg agguacuugg guugauguag uauuagaaca cgguggaugc      660

guaacaguaa uggcacaaga uaaaccuaca guagacauag agcuaguaac aacaacugua      720

ucgaauaugg cugaaguuag auccuauugu uaugaagcaa guauaucgga uauggcauca      780

gacaguagau guccaacaca aggagaagca uacuuagaua aacaaagcga uacacaauau      840

guauguaaaa gaacauuggu ugauagagga uggggaaaug gauguggauu auucggaaaa      900

ggaagucuag uaacuugugc aaaauucacg uguucaaaga aaaugacagg aaaaucaaua      960

cagccggaaa aucuagaaua uagaauaaug cuaucagucc auggaagcca gcauucagga     1020

augauaggau acgaaacaga ugaagauaga gcaaaaguag aaguaacucc aaauucacca     1080

agagcagaag caacuuuagg cgguuuuggu uccuuagguu uagacugcga accaagaaca     1140

ggauuagauu uuucagauuu guacuaccua acuaugaaca auaaacauug gcuaguucau     1200

aaagaauggu uucaugacau uccauuaccc uggcaugcug gagcagacac aggaacucca     1260

cauuggaaua auaaagaagc cuugguagag uuuaaagaug cacacgcaaa aaggcagacu     1320

guaguaguau uaggcucuca ggaaggggcg guacacacag cauuagcagg agcauuagaa     1380

gcagaaaugg auggagcgaa ggguagauua uuuaguggcc auuuaaagug uagauuaaaa     1440

auggauaaau uaagauuaaa aggaguauca uauagucuuu guacagcagc uuucacauuu     1500

acuaaagugc cagcagaaac auuacaugga acaguaacag uugaaguaca auaugcugga     1560

acagauggac cauguaagau accaguccag auggcaguag acaugcaaac ucuaacacca     1620

gucgggagau uaauuacugc aaacccggua auaacagaaa gcacagaaaa cagcaaaaug     1680

auguuagaau uagacccacc auuuggugac aguuauauag uaauaggugu aggagacaag     1740

aaaauuacuc aucacuggca ucgaagugga agcacucuag guaaagcauu cucuacaaca     1800

cuaaaaggag cacaaaggcu agcagcacua ggugacacag caugggacuu uggauccaua     1860

ggaggaguuu uuaacucaau agguagagcu gugcaucaag uauucggagg agcauuucgu     1920

acuuuauucg gaggaaugag uuggaucaca caaggauuaa ugggagcacu auuauuaugg     1980

augggaguca augcaagaga uagaucaaua gcacuagcau uuuuagcaac uggaggagug     2040

uuaguauuuc uagcuacuaa uguacacgcc                                      2070


<210>  9944
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9944
augggaaaaa gaucagcagg aaguauaaug ugguuagcaa gucuagcagu aguaauagca       60

ugcgcaggag cuguaacgcg aaggggauca gcauauuaua uguauuuaga uagaaaugac      120

gcaggagaag caauaucauu ucccacuaca uuaggaauga acaaauguua uauacaaaua      180

auggacuuag gacauaugug ugaugcaaca augucauacg aauguccaau guuagacgaa      240

ggcguagaac cagaugaugu agauugcugg ugcaauacua caaguacaug gguagucuac      300

ggaacauguc aucauaagaa aggagaagcc agaagaagca gacgagcagu uacacuacca      360

ucacauagua cgagaaaauu acaaacaaga ucacaaaccu ggcuagaaag uagagaauau      420

acaaaacauu uaauaagagu cgaaaauugg auauuuagaa auccaggauu ugcauuagca      480

gcggccgcaa uagcauggcu acuugguucu ucaacaucac aaaaaguaau auauuuagua      540

augauuuuac uaaucgcacc agcauacuca auaagaugua uaggcguaag caacagagau      600

uuuguagaag ggaugagugg aggaacuugg guagauguug uauuagaaca cggcgguugc      660

gucacaguaa uggcacaaga caaaccaaca guagauauag aauugguaac aacaaccgua      720

aguaauaugg cagaaguaag aucauauugu uacgaagcau caauaaguga uauggcuuca      780

gauucaagau guccuacuca aggagaggcu uacuuagaca aacaaucaga cacucaauac      840

gucuguaaaa gaacacucgu cgauagagga ugggguaaug gaugcggacu auuuggaaaa      900

ggaucauuag uaacaugugc aaaauucgca ugcaguaaga aaaugacagg aaaaagcaua      960

caaccggaaa aucuugaaua cagaauaaug cuuucaguuc auggaucaca acauagugga     1020

augauaguca augauacagg ucaugaaacc gaugaaaaua gagcaaaggu agaaauaaca     1080

ccgaacaguc caagagcaga agcaacacuc ggaggauuug gaucauuagg uuuagauugu     1140

gaaccaagaa cgggacuaga uuuuagcgau uuauauuacc uaacuaugaa uaauaaacau     1200

ugguuaguac auaaagaaug guuucaugau auaccuuuac ccuggcaugc aggagcagau     1260

acuggaacuc cucauuggaa uaauaaagaa gcauuaguag aauuuaaaga cgcacacgcu     1320

aaaaggcaaa caguuguggu uuuaggaagu caggaaggag caguacacac agcauuagcg     1380

ggagcauuag aagccgaaau ggacggagca aaaggccgau uaucaucagg acaucuuaaa     1440

uguagacuaa aaauggauaa acuaagauua aaaggaguuu cuuauucauu guguacagca     1500

gcauuuaccu uuacaaaaau accggcugaa acuuugcacg gaacggugac aguagaagua     1560

caauaugcag guacagacgg uccuuguaaa guaccagccc agauggcagu agacaugcaa     1620

acacuaacgc caguagguag auuaaucaca gcaaacccag uaauaacgga aagcacagaa     1680

aauucuaaaa ugauguuaga auuagaucca ccuuucggag acucauauau agucauagga     1740

gugggugaga aaaagauaac acaccauugg cacagaagcg gaaguacauu aggaaaagca     1800

uucucaacaa cacuaaaagg agcacaacgu cuagcugcau ugggugauac ugcaugggau     1860

uuuggcagca uagguggagu auuuaauagc auugguagag cugugcauca aguuuucgga     1920

ggagcauucc gaacauuauu uggcggaaug ucauggauaa cacaaggauu aaugggagca     1980

cuauuauuau ggaugggagu uaaugcaaga gauaggagua ucgcacuagc auucuuagca     2040

acaggaggag uauuaguguu uuuagcaacu aauguacacg cu                        2082


<210>  9945
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9945
augacaucag uuggaauagu aggccuacuu uuaacaacag caauggcagc aguuacauua       60

ccaagucaua guacaagaaa acuacagaca agaagccaaa caugguuaga auccagagag      120

uacacuaaac auuuaaucag gguagaaaau uggauauuua gaaauccagg auuugcacua      180

gcagcagccg caauagcaug gcuauuagga ucuucuacau cacaaaaagu aauuuauuua      240

guaaugauac uauuaauagc accugcuuau aguauaaggu guauuggagu uucaaaucga      300

gacuuuguug aaggaauguc cgguggaaca uggguagaca uaguauuaga acauggcggu      360

uguguaacug uaauggcgca agauaaacca acuguagaua uagagcuagu aaccaccaca      420

guaaguaaua uggcagaagu uagaaguuau uguuaugaag cgucaauaag ugacauggca      480

agugauagcc gauguccaac ucaaggcgaa gcauauuuag acaaacaauc agauacucaa      540

uauguuugca aaagaacauu aguagauaga gguuggggaa auggcugugg auuauuuggu      600

aaaggaucau uaguaacuug cgcaaaauuu gcauguucaa aaaaaaugac aggaaaaucu      660

auacaaccag aaaaucuaga auacagaaua augcuaagug uacacggauc ccaacauuca      720

ggaaugauug uaaaugauac uggacaugaa acagaugaaa auagagcaaa aguagaaauu      780

acaccaaacu caccucgagc agaagcaaca uuaggcgguu uuggaucuuu aggauuagau      840

ugcgaaccac guacagguuu agauuucuca gaucucuauu aucucaccau gaauaauaaa      900

cauuggcuag uacauaaaga augguuucau gauauuccau uaccauggca cgcgggggca      960

gauacaggca cuccacauug gaacaauaaa gaagcauuag uagaauuuaa agacgcccau     1020

gcuaaaagac aaacaguugu aguacuagga agccaagaag gagccguaca uacagcauua     1080

gcuggggcac uagaagcaga aauggaugga gcaaaaggaa gauuaaguuc uggucaucua     1140

aaauguagau uaaaaaugga caaacuacga uuaaaaggug uuagcuauag cuuauguaca     1200

gcagcauuua cauuuacaaa aauaccugca gaaacuuuac auggaacagu aacagucgaa     1260

guacaauacg caggaacaga uggcccaugu aaaguuccag cacaaauggc cguagacaug     1320

cagacauuaa cacccguagg aagauuaauu acugcaaauc caguaauaac ggaauccaca     1380

gaaaacucaa aaaugauguu agagcuugac ccaccguuug gagauaguua uauagugaua     1440

ggaguaggag aaaaaaaaau aacucaccau uggcacagaa gugguucuac aauaggaaaa     1500

gcauucgaag caacggucag aggugcaaaa agaauggcug uauuaggaga cacagcaugg     1560

gacuuuggca gcguaggagg agcacuaaac ucauuaggaa aaggaauaca ccaaauuuuc     1620

ggagcggcau ucaaaagucu uuuuggagga augucuuggu uuucacaaau auuaauagga     1680

acacuacuaa ugugguuagg acuaaauaca aaaaauggau caauaucccu gaugugccua     1740

gccuuaggag gcguacuaau auuuuuaucc acugcaguau cagcu                     1785


<210>  9946
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9946
augacaagcg uaggaauagu aggauuauua uuaacaacag caauggcugc ugucacccua       60

ccaagucaua guacaagaaa acuacaaacu agaucacaaa cauggcuaga aagucgagaa      120

uauaccaaac auuuaauaag aguggaaaau uggaucuuua gaaauccagg auuugcucug      180

gcugcagcgg caauagcaug guuacuugga ucaaguacau cacaaaaagu aauauauuua      240

guuaugauau uacuuauagc accagccuau aguauacguu guauaggcgu uagcaauaga      300

gauuuugucg aaggaaugag cggaggaaca uggguagacg uaguacuaga acauggagga      360

uguguaaccg uaauggcaca agacaaaccu acgguggaua uagaauuagu aacgacaaca      420

guaucuaaua uggcagaggu acgaagcuac uguuaugaag caaguauauc agauauggca      480

agugauagca gauguccaac acaaggagag gcauaccuag auaaacaaag ugauacacaa      540

uaugugugca aaagaacccu aguagaucga ggcuggggaa auggcugugg uuuauuuggu      600

aagggaucau uaguaacaug ugcaaaauuu gcaugcucua agaaaaugac aggaaaauca      660

auacaaccug aaaaucuuga auauagaaua auguugagug uacauggauc ucaacauagc      720

ggaaugauug ugaaugacac aggacacgag acagaugaaa auagagcuaa aguagaaaua      780

acaccaaauu caccaagagc agaagcaaca uuaggaggau ucggaucguu agguuuagau      840

ugugaaccca gaaccggauu agauuuuagc gauuuauauu accuaacaau gaacaauaaa      900

cauugguuag uacauaaaga augguuucau gauauucccu uaccuuggca ugcuggagca      960

gauacaggaa caccacacug gaacaauaaa gaagcauuag uagaauuuaa agaugcacac     1020

gcaaaaagac aaacuguggu aguacuagga agucaagaag gugcaguaca cacagcauua     1080

gcaggagcau uagaagcaga aauggacgga gcuaaaggaa gauuaucuuc aggacaccua     1140

aaguguagau uaaaaaugga caaacugaga cuaaaaggug uaaguuauag uuuauguacc     1200

gcagcuuuua cauuuacaaa aauaccagca gaaacacuac acggaacagu aacggucgaa     1260

gugcaauaug caggaacaga uggaccaugc aaagugccag cacaaauggc gguagacaug     1320

caaacauuaa caccaguagg aagauuaauu acugcaaauc caguuauaac cgaaagcaca     1380

gaaaacucaa aaaugauguu agaacuagau ccaccuuuug gagauaguua uauaguaaua     1440

ggaguaggug aaaagaaaau cacacaccau uggcauagaa guggcucaac aauaggaaaa     1500

gcauucgaag caacaguaag aggggcaaaa agaauggcug uacuaggaga uacagcaugg     1560

gauuuuggau cagucggagg agcauuaaac agcuugggaa aaggaauaca ucaaauauuu     1620

ggagcugcau uuaagaguuu auucggcgga augaguuggu uuagucaaau auuaauagga     1680

acuuuacuca ugugguuagg guuaaaugcc aaaaauggcu cgauuagucu aauguguuua     1740

gcuuuaggag gaguauuaau auuucuaagc acagcggucu cagcu                     1785


<210>  9947
<211>  1773
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9947
augacaagua uaggaauaau aggacuacua uuaacaacag ccauggcagc aguaacacua       60

ccuucacacu cgaccaggaa acuacaaacc cgaagucaaa ccugguuaga aaguagagaa      120

uacacuaaac aucuaauaaa aguugaaaau uggauauuua gaaauccagg auuugcuuug      180

guugcaguug cuauagcaug gcuauuagga agcagcacau cucaaaaagu uauuuaucua      240

guuaugauac ucuuaauagc uccugcauau aguauacgcu guauaggagu aucaaauaga      300

gauuuuguag aaggaauguc aggaggcaca uggguagaug uuguacuaga acacggugga      360

uguguaacag uaauggcaca agauaaacca acgguagaca uagaauuagu aacaacaacc      420

guaaguaaua uggcagaagu acgaucauac ugcuaugaag caucaauaag cgacauggca      480

agugauucua gaugcccaac acaaggugaa gcauaucuag auaaacaauc ugacacgcag      540

uauguaugua aacgcacgcu aguagauaga ggauggggua acgguugugg ucuauuuggu      600

aaaggaucuc uuguaacaug cgcaaaauuc acauguucua agaaaaugac aggaaaaagc      660

auucagccag aaaaucuaga auacagaaua augcuaucug uucauggauc ucaacauucc      720

gguaugauag gauaugaaac ugaugaagau agagcuaaag uagaaguaac accaaauagu      780

ccaagagcug aagcaacacu aggaggcuuc gguaguuuag gguuagauug ugaaccgaga      840

acaggacuug acuuuaguga ccuauauuau cuuacaauga acaauaaaca uugguuaguc      900

cauaaagaau gguuccacga uauaccacua ccauggcaug cuggugcaga uacaggaaca      960

ccacacugga auaauaaaga agcucuagua gaguucaaag augcacaugc aaaaagacaa     1020

acaguaguag uacuaggaag ucaagaaggg gcaguacaua cagcauuagc aggagcauua     1080

gaagcagaaa uggacggagc aaaaggacgu cucuucuccg gacaccuaaa augcaggcua     1140

aaaauggaca aauuaagguu aaaaggagua ucuuauucac uuuguacagc cgcauucaca     1200

uuuacaaaag uaccagcaga aacacuacau ggaacuguaa caguugaagu ucaauaugcu     1260

ggaacagaug gaccauguaa aauaccugua caaauggcag uagauaugca aacuuuaaca     1320

ccaguaggua gauuaauaac agcaaacccu guuauaacag aaaguacaga aaacagcaaa     1380

augaugcuag aacuagaccc uccauucgga gacucuuaca uaguaauugg aguaggagac     1440

aagaaaauaa cacaucauug gcaccgaucu ggcucaacaa uaggaaaagc auuugaagca     1500

acaguaagag gagcaaaaag aauggcagua uuaggagaua cugccuggga uuuuggauca     1560

guaggaggag uauucaauag cuuaggcaaa ggcauacauc aaauauuugg ugcugcauuc     1620

aaaaguuuau uugggggaau gucaugguuu ucacaaauuc uaauaggaac acuauuagua     1680

uggcuaggau uaaacacgaa aaauggaagc auuagucuaa caugucuagc ucuaggugga     1740

guaaugauau uccuaucaac agcaguauca gca                                  1773


<210>  9948
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9948
augacaucag uaggaauagu cggauuauua uuaacaacag caauggccgc aguaacauua       60

ccaagccaua guacuagaaa auuacaaaca cgaagccaaa caugguugga aucaagagaa      120

uacacaaaac aucuuauaag aguggaaaau uggauauuca gaaauccagg auuugcacua      180

gcagcagcag cuauagcaug gcuucuaggc aguaguacau cacaaaaagu cauuuauuua      240

guaaugauau uacuuauagc uccagcauau ucuauucguu guauaggagu aucaaacaga      300

gacuuuguug aaggaaugag uggaggaacu uggguagaug uaguuuuaga acauggugga      360

uguguaacag uaauggcaca agauaaaccu acaguagaua uagagcuagu uacaacaacg      420

guaucaaaua uggccgaagu aaggaguuau uguuaugaag caucaauauc agauauggca      480

ucagauagua gauguccaac acagggcgaa gccuaucuag auaaacaauc agacacacaa      540

uauguaugua aaaggacacu gguggauaga ggauggggca acggaugugg cuuauuugga      600

aaagguaguu uagucacaug ugcaaaauuu gcauguucua agaaaaugac agguaaaucu      660

auccaaccag aaaaucuaga auauagaaua augcuuagcg uucauggaag ucaacacagu      720

gguaugauug ugaaugauac uggacaugaa acagacgaaa auagagcaaa aguagaaaua      780

acaccaaacu cacccagagc ugaagcaaca cuaggaggau ucggaucacu uggacuagau      840

ugugaaccua gaacuggacu ugauuucagu gauuuauacu acuuaacuau gaauaauaaa      900

cauugguuag uacauaaaga augguuccac gacauuccac uaccuuggca ugcuggugca      960

gauacaggaa cgccacauug gaauaauaaa gaagcacucg uagaguucaa agaugcacau     1020

gcaaaacgac aaacgguagu uguauuagga agucaagaag gcgcagucca uacagcauua     1080

gcuggagcac uagaagcuga aauggaugga gcaaaaggua gauuaaguuc cggacaccua     1140

aaauguagac uaaaaaugga uaaauuaaga cugaaaggug uaagcuauag ucugugcacc     1200

gcugcauuua cauucacaaa aauaccugcu gaaacauuac auggaacagu aacaguugaa     1260

guacaauaug caggaacaga cggaccuugc aaaguacccg cacagauggc uguagacaug     1320

caaacauuaa ccccaguggg aagacuaaua acagcaaauc caguaauaac agaaagcacu     1380

gaaaauucaa aaaugauguu agaacucgau ccaccauuug gggauagcua uauaguaaua     1440

ggaguaggcg aaaagaaaau aacacaccac uggcauagaa gugguaguac cauuggaaaa     1500

gcauuugaag cuacagucag aggagcaaaa agaauggcag uacuuggcga uacagcuugg     1560

gauuuugguu cuguaggcgg agcuuuaaac aguuuaggaa aaggaauaca ucaaaucuuu     1620

ggagcagccu uuaaaaguuu auuuggagga augaguuggu uuucccaaau uuuaauagga     1680

accuuauuaa uguggcuagg uuuaaauacg aaaaauggga gcaucagcuu aaugugucua     1740

gcacuuggug ggguauuaau auuucuaagc acagcgguau cugca                     1785


<210>  9949
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9949
auggccauaa gcggaguacc aguauuagga uucuucauaa ucgcaguacu aaugagugca       60

caagaaaguu gggcagcagu gacauuacca agucacagua caagaaaauu acaaacacgu      120

agucaaacau gguuagaaag ucgagaauau acaaaacauc uaauacgugu agaaaacugg      180

auauuuagaa auccaggauu ugcuuuggca gcagcugcca uagcauggcu ucuaggaagc      240

ucaacaucac aaaaaguuau auaucuaguc augauucuuc uaaucgcccc ugcauauuca      300

auaagaugua uaggagucuc aaacagagau uucguagaag gaaugucagg aggaacaugg      360

guagacauag uccuugaaca cggaggaugu guaacuguua uggcccaaga uaaaccuacu      420

guagauauag aauuggucac aacuacagua agcaacaugg cagaaguaag aucauauugu      480

uaugaagcua gcauaucaga uauggcauca gauucaagau gcccaacaca aggugaagca      540

uaucuagaua aacaaucaga uacacaauac guauguaaac gaacauuagu agauagagga      600

uggggaaaug gauguggacu uuuuggaaaa ggaucauuag uaacaugcgc caaauuugcg      660

uguucaaaga aaaugacugg aaaaucaaua caaccggaaa accuagaaua uagaauaaug      720

uuaaguguuc auggaucaca acauucaggu augauaguua augauacagg acaugaaaca      780

gaugaaaaca gagcaaaagu agaaauaaca ccaaauucac caagagcaga agcaacauua      840

ggcgguuucg gaaguuuagg acuugauugu gaaccuagaa cagguuuaga uuuuucagac      900

cuauauuauu ugacaaugaa uaacaaacac ugguuaguac acaaagaaug guuccaugau      960

auaccacuac cauggcaugc uggugcggau acaggaacuc cccauuggaa uaauaaagaa     1020

gcguuaguag aauuuaaaga cgcacacgca aaaagacaaa cuguaguagu auugggaucu     1080

caagaaggag caguacacac ugcauuagca ggagcacuag aagcagaaau ggauggagcu     1140

aaaggcagac uuagcagugg acauuuaaaa ugucguuuaa aaauggacaa auuaagauua     1200

aaagggguau ccuacucccu augcacagcu gcuuuuacuu ucacaaaaau uccugcagaa     1260

acacuucaug gaaccguaac aguagaagua caauacgcgg gaacagaugg gcccugcaaa     1320

guacccgcac aaauggcagu agauaugcaa acauuaacac cuguaggcag auugauaacu     1380

gcuaauccag uaauaacaga aucaacagaa aauaguaaaa ugaugcuaga auuagaccca     1440

ccauuuggcg auaguuauau agucauugga guuggggaga agaaaauaac ucaucacugg     1500

cacagaucag gaaguacuau aggaaaagca uucgaagcaa cagugagagg agcaaaaaga     1560

auggcaguau uaggugauac agcuugggau uuuggaucag uaggaggagc acuaaacucu     1620

cuagguaaag gaauccauca aauuuuuggu gcagccuuua aaucauuauu uggaggaaug     1680

ucaugguuca gucaaauauu aauagguaca uuauuaaugu ggcugggacu aaauacuaaa     1740

aacgguagua uaagccuaau gugucuagca cucgguggug uacuaauauu cuuaaguaca     1800

gcagucucag ca                                                         1812


<210>  9950
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9950
auggcgauaa guggaguacc aguguuagga uuuuuuauaa uagcaguacu aaugucugcu       60

caagaaucau gggcagcagu cacauuacca ucacacucaa cuaggaaauu acagacccga      120

ucgcaaaccu ggcuggaauc aagagaauau acaaaacacu uaauaagagu agaaaauugg      180

auauuuagaa aucccggauu cgcacuagca gcggcugcaa uugcuugguu acuuggaucc      240

aguacauccc aaaaaguaau auauuuagua augauacuuc uuaucgcacc agccuauagu      300

auuagaugua uuggaguauc aaauagagau uuuguagaag gaaugucagg agguacuugg      360

guugaugugg uacuagaaca cggcggaugu gucacaguaa uggcacaaga uaaacccaca      420

guagauauag aauuaguaac gacaacagug ucaaauaugg cagaaguaag aucauauugc      480

uacgaagcaa guauaaguga cauggcauca gauucacgau guccaacaca aggagaagca      540

uauuuagaua aacaaucaga cacacaauau guauguaaaa gaacauuagu cgauaggggu      600

uggggaaacg gaugcggcuu auuuggaaaa ggauccuuag uaaccugugc aaaauucgca      660

uguucgaaaa agaugacagg aaaaucuaua caaccagaaa auuuagaaua uagaauaaug      720

cuaucaguac acggaucuca acauucaggu augauaguaa augauacugg acaugaaaca      780

gacgagaaca gagcaaaagu agaaauaacu ccaaauagcc cacgugcgga ggccacacua      840

ggaggauuug gaagccuggg gcuagacugu gaaccuagaa caggacuaga uuucucagac      900

cuauauuauu uaacaaugaa uaacaaacau ugguuagucc acaaagaaug guuucaugau      960

aucccacuac cauggcacgc aggugcagau acggguacac cacauuggaa uaacaaagaa     1020

gcauuagucg aauuuaaaga ugcccaugca aaaagacaaa caguaguagu acuagguuca     1080

caagaaggag cugugcauac agcacuagcu ggugcauuag aagcagaaau ggauggagca     1140

aaaggaagau uaucaagcgg acauuugaaa ugucgacuaa aaauggauaa acuuaggcuu     1200

aaagguguaa gcuacucuuu auguaccgca gcauucacgu ucacaaaaau accagccgaa     1260

acauuacacg guacuguaac aguagaagua caauacgcag guacagaugg accauguaaa     1320

guaccagcuc aaauggcagu agacaugcaa acuuuaacac caguagggag auuaauaaca     1380

gcgaauccag uaauuacaga auccacagaa aacaguaaaa ugaugcuaga auuagaucca     1440

cccuuuggag acucauauau cguaauaggu guaggugaaa aaaaaauaac acaucacugg     1500

caucgaucug gaucaaccau aggaaaagca uuugaagcua caguaagagg agcgaaaagg     1560

auggcaguuu uaggagacac ugcaugggau uuugguagug uaggaggagc auuaaauuca     1620

uuggguaaag gcauacauca aauauucgga gcagcauuua aaagcuuauu uggaggaaug     1680

aguugguuuu cacaaauacu aauaggaacu uuacuuaugu gguuaggucu aaaugcuaaa     1740

aauggcucaa uaucacuaau guguuuagca cuagguggag uacuaauauu uuuaaguaca     1800

gcuguaucag ca                                                         1812


<210>  9951
<211>  1800
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9951
auggcaauau cagguguacc agucuuaggu uucuuuauaa uagcaguauu aaugucagca       60

caagaaagcu gggccgcagu aacucuaccc agucacucga caaggaaacu acaaaccaga      120

ucacaaacau gguuagaaag uagagaauau acaaaacacc uuauuaaagu agaaaauugg      180

auauuuagaa auccuggguu cgcguuaguc gcaguagcaa uagcauggcu auuaggaagc      240

agcacaaguc aaaaaguaau auaccuagua augauauuac uaaucgcgcc agcauauagu      300

auacguugua uaggaguuuc aaacagagac uuuguagaag gaaugucagg agguacaugg      360

guagaugucg uauuagaaca uggaggaugu gucacaguua uggcucaaga uaaaccaaca      420

guagauaucg aauuaguaac aacuacuguc aguaauaugg ccgagguuag aucauacugu      480

uacgaagcau caauaucaga uauggcaagu gauaguagau gcccaacaca aggagaagca      540

uauuuagaca aacaaucaga uacucaauac guauguaaac gaacacuagu agacagaggu      600

uggggaaacg gauguggauu auuuggaaaa ggaaguuuag uaacaugugc aaaauuuacu      660

ugcucaaaaa aaaugacugg aaaaucaauu caaccggaaa aucuagaaua ucgaauaaug      720

cuaaguguac auggaagcca acauucagga augauuggcu augaaacuga ugaagacaga      780

gcuaaaguug aagugacacc aaacucaccu agagcagaag caacauuagg agguuucggu      840

ucauuaggau uagauugcga accacgaaca gguuuagauu ucagugaucu auauuauuua      900

acaaugaaca auaaacauug gcuaguacau aaagaauggu uccaugauau accacuccca      960

uggcaugcgg gugcagacac aggcacucca cacuggaaca auaaagaagc uuuaguagaa     1020

uuuaaagaug cacacgcaaa aagacagaca guaguuguac uaggaucaca agaaggagca     1080

guacacaccg cacuugcagg cgcauuagaa gcggaaaugg acggagccaa agguagauua     1140

uuuaguggac auuuaaaaug uagauuaaaa auggauaaau uaagacuaaa aggaguaagc     1200

uacucauuau gcacagcagc cuuuacauuu acaaaaguac ccgcugaaac auugcacgga     1260

acaguuacag uugaaguuca auacgcagga acagauggac cauguaaaau accaguacaa     1320

auggcagucg acaugcaaac auuaacacca guaggucgac uaauaacagc aaaccccguu     1380

auaaccgaau cuacugaaaa cucgaaaaug augcuagaac uagacccacc guuuggagau     1440

ucauauauag uaauaggcgu aggagauaaa aaaauaacac aucauuggca cagauccgga     1500

agcacaauag gaaaagcauu ugaagcuaca guaagaggag cuaaaagaau ggcaguauua     1560

ggagauacag caugggacuu uggaagugua ggagguguau uuaacaguuu aggaaaagga     1620

auacaucaaa uauuuggcgc agccuuuaaa agccuauucg gaggaaugag cugguuuuca     1680

caaauucuaa ucggaacucu acuagugugg cuaggacuua auacaaaaaa ugguagcaua     1740

aguuuaacau gucuagcauu aggagguguu augauauuuc uaucaacagc gguaagugca     1800


<210>  9952
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9952
auggcaauuu cuggaguucc aguauuaggu uuuuuuauua uagcgguacu aaugagugca       60

caagaaagcu gggcagcagu aacauuaccu agucauagua cuagaaaacu acaaacaaga      120

ucacaaacau gguuagaauc gcgagaauau accaaacauu uaauaagagu agagaacugg      180

auuuucagaa auccaggcuu cgcccuagca gcagcagcaa uagcaugguu auuaggaagu      240

ucaacaucac aaaaaguuau auaucuaguc augauauuau ugauagcgcc cgcauauuca      300

auacgaugua ucggaguauc aaauagagau uuuguagaag gaaugucugg aggaacuugg      360

guagacgucg uacuagaaca cgggggaugu guaacaguaa uggcucaaga uaaaccuaca      420

guagauauag aauuaguaac gacaacagua agcaacaugg cagaagucag auccuauugu      480

uaugaagcca guauuagcga cauggcgagc gauagcagau guccaacaca aggggaagcu      540

uauuuagaua aacaaucaga cacacaauac guauguaaaa gaacauuagu agauagaggu      600

uggggaaaug guuguggacu uuuuggaaaa ggaaguuuag uaacaugugc aaaauuugca      660

ugcucuaaaa aaaugacagg caaaucaaua caaccagaga accucgaaua uagaauaaug      720

uuaagcguac auggaaguca acauagcggu augauaguaa augacaccgg acacgaaaca      780

gacgaaaaua gagcaaaagu agaaauaacg ccaaauaguc cgcgcgcaga agcaacauua      840

ggaggauucg gaucacuagg auuagacugu gaaccaagaa caggacuuga uuuuucugau      900

cuauacuauc uuacaaugaa uaauaaacau ugguuaguuc auaaagaaug guuccaugau      960

auuccuuuac cauggcacgc aggugcagau acaggaacac cacauuggaa uaauaaagaa     1020

gcauuaguag aauucaaaga ugcucaugcu aaacgucaaa cuguaguugu auuaggaagc     1080

caagaaggag caguucauac ugcauuagca ggagcauuag aagcugaaau ggauggugca     1140

aaagguagau ugaguagugg acauuuaaaa uguagacuaa aaauggacaa auuacgacua     1200

aaaggaguuu cauauagucu auguacugcu gcauuuacau ucacaaaaau accagcugaa     1260

acauuacacg gaaccguuac aguugaagua caauacgcag gcacagaugg accaugcaaa     1320

guuccagcac aaauggcagu agacaugcaa acauuaacac caguaggaag acuaauuaca     1380

gcaaauccug uaauaacaga aucaacugaa aauagcaaaa ugauguuaga auuagaucca     1440

ccauuuggag auucauauau ugugauagga guaggagaaa aaaaaauuac acaccacugg     1500

cauagaucug ggucaacaau agguaaagca uucgaagcua cuguaagagg agcaaaaaga     1560

auggcaguac uuggagauac cgccugggau uucgguagug uaggaggagc acuaaacuca     1620

cuuggaaaag gcauacauca aauauuugga gcagcauuua aaucacuauu ugguggaaug     1680

ucaugguuuu cacagaucuu aauagguaca cuacuaaugu ggcuaggacu uaauacaaaa     1740

aauggaucaa uaucauuaau guguuuagca uuaggaggag uuuuaauuuu uuuaagcaca     1800

gcaguaagug ca                                                         1812


<210>  9953
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9953
auggauugga cauggauauu auucuuaguu gcagcugcaa caagaguaca uagugcagua       60

acauugccgu cacacucaac aaggaaauua caaacaagaa gccaaacaug guuagaaagc      120

cgagaauaca caaaacauuu aauuagagua gaaaacugga uauuuagaaa uccaggauuu      180

gcucuagcgg cagcagcaau agcaugguua cuuggaucau caacaagcca gaaaguaaua      240

uacuuaguaa ugauacuucu uauagcucca gcauacucaa uacgauguau uggaguaagc      300

aauagagauu uugucgaagg aaugucagga ggaacauggg ucgacaucgu auuagaacau      360

gguggaugug uuacaguaau ggcacaagau aaaccuacug uagauauaga acucguaacu      420

acaacuguau caaacauggc agaaguaaga ucauacugcu augaagcauc aauaagugau      480

auggcaucgg auaguagaug uccuacacaa ggagaagcau auuuagauaa acaaucagau      540

acacaauacg uauguaaacg uacuuuagua gauagagguu ggggaaaugg augcggauua      600

uuuggaaaag guaguuuagu aacaugugca aaauuugcuu guucaaagaa aaugacaggu      660

aaaaguauac aaccagaaaa uuuggaauau agaauaaugu uaucuguaca uggaagccaa      720

cauaguggaa ugauuguaaa ugauacagga caugagaccg augaaaauag agcaaaagua      780

gaaauaacuc caaauucacc acgagcagaa gcgacauuag gaggauucgg uucauuaggu      840

uuagauugug aaccaagaac agggcuagau uuuucugauc uauauuaucu aacuaugaac      900

aauaaacauu ggcuaguaca uaaagagugg uuucaugaca uaccauuacc auggcaugcu      960

ggagcagaua caggaacacc acauuggaac aauaaagagg cuuugguuga auuuaaagac     1020

gcccacgcaa aaagacagac aguagucguu uuaggcaguc aagaaggagc aguucacaca     1080

gcauuagcug gagcauuaga agccgaaaug gacggagcaa aaggucgauu aucaagugga     1140

caccuaaaau guagauuaaa aauggacaaa cuacgacuua aagggguuuc auauucacua     1200

uguacagcag ccuuuaccuu uacaaaaaua ccagcagaaa cuuuacaugg uacaguaaca     1260

gucgaagucc aauaugcagg uacagauggc ccauguaaag ucccggcaca aauggcagua     1320

gauaugcaga cacuaacccc aguaggaaga cuuauaacag caaauccagu uauaacagaa     1380

ucuacugaaa auucaaaaau gauguuagag uuagauccuc cauuuggaga uagcuauauc     1440

guaauaggag ucggagaaaa gaaaauaaca caucauuggc auagaagugg aaguaccaua     1500

ggaaaagcau uugaagcaac aguaagagga gccaaaagaa uggcaguacu uggagauacg     1560

gcaugggauu uuggcagcgu aggaggugca uugaauaguu uaggaaaagg aauacaucaa     1620

aucuuuggug ccgcauucaa auccuuauuu ggaggaauga gcugguuuuc acaaauacua     1680

aucggaacau uacuaaugug guuaggauua aauacuaaaa auggaagcau aaguuuaaug     1740

ugccuggcac uaggaggagu acuaauauuu uuaaguacag cagugagcgc u              1791


<210>  9954
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9954
auggauugga cauggauauu auucuuagua gcugcagcaa cccgaguaca cucagcaguc       60

acauuaccca gucauagcac aaggaaauua caaaccagau cacaaacuug guuagaauca      120

agagaauaca caaaacaucu cauaagaguu gaaaacugga uauuuagaaa ccccggcuuu      180

gcucuagcag cagcagcaau ugcauggcua cuaggaagcu cuacaucaca aaagguaaua      240

uauuuaguaa ugauacuauu aauagcacca gcuuacagua uuagauguau aggaguuucg      300

aauagagacu uuguagaagg aaugucagga gguacauggg uagauguagu acuagaacau      360

ggcggaugug ugacaguuau ggcacaagau aaaccaaccg uagauauaga auuagugacg      420

acaacaguau caaauauggc cgaaguaaga ucuuauuguu augaagcauc cauuucugac      480

auggccucag auaguagaug ccccacucaa ggagaagcau acuuagauaa acagucagac      540

acucaauacg uuuguaaaag aacucuagua gauagagguu gggguaaugg auguggauua      600

uuuggaaaag gaagccuagu aacaugcgca aaauuugcau guaguaaaaa gaugacugga      660

aaaucaauac aaccagaaaa ccuugaauau cgaauuaugu ugucagucca cggaagucaa      720

cauaguggaa ugauaguaaa ugacacagga cacgaaacag acgaaaauag agcuaaagua      780

gaaauaacac caaauucucc gagagcugaa gcaacacuug gaggauuugg uagcuuaggu      840

uuagacugug aaccaaggac uggauuggac uuuucagauc uauauuauuu aacaaugaau      900

aauaaacacu ggcuaguaca caaagaaugg uuucacgaua uuccauuacc auggcaugca      960

ggagcugaca cagguacacc acauuggaau aauaaagaag cauuaguaga guucaaagau     1020

gcacacgcga aaagacaaac aguaguagug cuagguucac aagaaggagc aguacauacc     1080

gcauuagcag gagcauuaga agcagaaaug gauggagcaa aagggagacu aucaagugga     1140

cauuuaaaau guagauugaa gauggauaaa uuaagauuga aagggguauc uuacaguuua     1200

uguacugcag cuuucacauu uacaaaaaua ccagcagaaa cacuacaugg aacaguaaca     1260

guugaaguac aauacgcagg aacagaugga cccugcaaag uuccagcuca aauggcagua     1320

gauaugcaaa cucuaacgcc aguaggacgg uuaauaacag ccaauccagu gauuacagaa     1380

ucgacagaaa acucaaaaau gaugcucgaa uuagauccac ccuuuggaga uucauauauu     1440

guuauaggag uaggagaaaa gaaaauaaca caucauuggc auaggagcgg cucaacuaua     1500

ggaaaagcau ucgaagcaac aguaagagga gcuaaacgaa uggcaguauu aggagauaca     1560

gcuugggacu ucggaagcgu uggaggagcc cuaaauaguu uaggaaaagg aauacaucaa     1620

auauucggag cagcauuuaa aucauuauuu ggaggaauga guugguucuc acaaauucuu     1680

auuggaaccc uauuaaugug gcuaggcuug aaugcuaaaa auggaaguau aagucucaug     1740

ugcuuagcac uaggaggggu auuaauauuu uuauccaccg caguuucagc a              1791


<210>  9955
<211>  1779
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9955
auggauugga cauggauauu auuuuuaguc gcagcagcaa cuagaguaca uucugcaguu       60

acuuuaccaa gucauucaac aaggaaacua caaacaagau cacaaacaug gcuagaauca      120

agagaauaca cuaaacaucu aauaaaagua gaaaauugga uauuuagaaa ucccggcuuu      180

gcacucgugg caguagcaau agcaugguug uuagggagcu cuaccaguca aaaaguuauu      240

uaucuuguaa ugauacuacu aauagcacca gcauauagca uaagauguau aggaguuagc      300

aacagagacu uuguggaagg aaugucagga ggaacauggg uagaugucgu acuagaacau      360

ggcggaugug uaacaguaau ggcacaagau aaaccaacag uagauauaga acuaguaaca      420

acaacuguau cuaauauggc agaaguaaga ucauacuguu augaagcaag cauuucugau      480

auggcaucag auagcagaug cccaacacaa ggagaagcau aucuagauaa acaaagugau      540

acacaauacg uaugcaaaag aacauuaguu gacagaggau gggggaaugg uugcggucug      600

uuuggaaaag gaaguuuagu uacaugugcu aaauuuacau gcaguaaaaa aaugacugga      660

aaaagcauuc aaccagaaaa uuuagaauac cgaauaaugu uaagcguaca uggaagccaa      720

cacucaggaa ugauaggaua ugaaacagau gaagauagag cuaaaguaga agucacaccg      780

aauucaccaa gagcugaagc aacucuagga gguuuugguu cacuugguuu agauugugaa      840

ccaagaacag gacuagacuu cucagauuug uauuaccuaa caaugaauaa uaaacauugg      900

cuaguucaua aagaaugguu ucacgacaua ccauugccuu ggcacgcagg agccgauacu      960

ggaacaccac auuggaauaa uaaagaagca cucguagaau uuaaagaugc acaugccaaa     1020

agacaaacug uagucguacu aggaagucaa gaaggggccg uacauacugc auuagcaggc     1080

gcacuagaag cugaaaugga uggcgcaaaa gguagacuau ucagcggaca ccuaaaaugu     1140

cgauuaaaaa uggauaaauu aagauuaaag ggaguaagcu auucuuuaug uacagccgcu     1200

uuuacauuca cuaaaguacc agcagaaaca uuacacggua caguaaccgu agaaguccaa     1260

uacgcaggaa cagauggacc augcaaaauu ccaguacaaa uggcaguaga uaugcaaacg     1320

uuaacaccag uaggucgauu aauuacagca aauccaguaa ucacagaauc aacagaaaau     1380

ucgaaaauga ugcucgaacu agacccucca uucggagaua guuauauagu aaucggagua     1440

ggagauaaaa aaauaacaca ccacuggcau agaaguggcu caacaauagg gaaagcauuu     1500

gaagcaacag uaagaggagc aaaaagaaug gcaguuuuag gugacacugc augggacuuu     1560

gguaguguag gagguguauu uaauagcuua ggaaaaggaa uccaucaaau auuuggagca     1620

gcauucaaau cauuauucgg ugguauguca ugguucucac aaauucuaau agguacacuu     1680

cuuguuuggu uaggucuaaa uacaaaaaau ggaucaauuu cucuaacaug uuuagcuuua     1740

ggagggguca ugaucuucuu aucuacggca guaucagcu                            1779


<210>  9956
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9956
auggacugga cauggauucu auuucucgua gcagcagcaa caagaguuca uucagcagua       60

acauuaccau cacauucaac aagaaaacua caaacaagaa gucaaacaug gcuagaaucg      120

agagaauaua caaagcauuu aaucagagua gaaaacugga uauuuagaaa cccuggauuu      180

gcauuggccg cugcugcaau agcaugguua cuuggaagca gcacaaguca aaaaguaaua      240

uaucuaguaa ugauacuauu aauagcccca gcauauucaa uuagauguau aggagucuca      300

aauagggauu ucguagaagg aaugagugga ggaacauggg uagacguagu acuagaacac      360

ggugggugcg uaacaguaau ggcacaagac aaacccacag uagauauaga auuagugaca      420

acuacaguua guaacauggc agaaguuaga aguuauuguu acgaagcaag cauaagugau      480

auggcaagcg auucucgaug uccgacucag ggagaagcau aucuagacaa acaaucugau      540

acacaauaug uguguaaacg aacucuaguc gauagagggu ggggaaaugg auguggacuc      600

uuuggaaaag gaagucuagu aacgugcgcu aaauuugccu guaguaagaa aaugacggga      660

aaauccauac aacccgaaaa cuuagaauau agaauaaugu uaagugugca uggaagucaa      720

cauaguggaa ugauaguaaa cgauacagga cacgaaacag augaaaauag agcaaaagua      780

gaaauaacac ccaauagccc cagagcagaa gcaacacuag gagguuuugg aucauuagga      840

cucgauugug aaccuagaac uggguuagau uuuucagauu uauauuauuu aacaaugaau      900

aauaaacauu gguuaguuca uaaagaaugg uuucaugaua uaccuuuacc auggcaugcu      960

ggggcagaca caggaacacc acauuggaau aacaaagaag cauuaguaga auuuaaagac     1020

gcacaugcua aaagacaaac ugugguagug cuagggaguc aagaaggggc aguacauaca     1080

gcauuagccg gagcuuuaga agcagaaaug gauggagcaa aaggaagauu auccagcgga     1140

caucuaaaau guagauuaaa aauggauaaa uuacguuuaa aaggugugag cuacucacuu     1200

uguacagcug cauuuacauu cacaaaaauu ccagcagaaa cccuacacgg aacagucaca     1260

gucgaaguac aauacgcagg aacagacgga ccuugcaaag uacccgcuca aauggcaguc     1320

gauaugcaaa cauuaacacc uguagguaga cuaauuacag caaauccagu aauaacagaa     1380

ucaacagaaa auaguaaaau gaugcucgaa uuagauccac cauuugguga uucauauaua     1440

guuauaggag uaggagagaa aaagauuaca caucauuggc auagaagugg aaguacaaua     1500

ggaaaagcau uugaagcaac aguaagagga gcaaaacgaa uggcaguauu aggagauaca     1560

gcuugggacu uuggaagugu uggaggugca cuaaauaguu uaggaaaagg aauacaucaa     1620

auauuuggag cagcauuuaa aucauuauuu ggugguaugu cgugguuuuc ccaaaucuua     1680

auaggaacuu uauuaaugug gcuaggauua aauacgaaaa auggaucaau aucacucaug     1740

ugucuagcau uaggaggagu uuuaaucuuu uuaucaaccg caguuucagc a              1791


<210>  9957
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9957
augggaaaaa gguccgcagg aaguauaaug ugguuagcua gcuuagcagu agugauagca       60

ugugcaggag cugcaguaac auuaccauca cauuccacaa gaaaacuaca aacaagaucu      120

caaacauggu uagaaucaag agaauacaca aaacaccuaa uacgaguaga aaacuggaua      180

uuuagaaauc cuggauucgc acuagccgca gcagcaauug ccugguuauu aggaaguagu      240

acaagccaaa aaguaauaua uuuaguaaug auauuauuaa uugcaccagc auauaguaua      300

agauguauag gaguaaguaa uagagauuuu guagaaggaa ugaguggagg uacaugggua      360

gauauaguau uagaacacgg aggaugugua acaguuaugg cucaagauaa accuacaguu      420

gauauugaau uaguaaccac uacaguauca aauauggcug aaguaagaag uuauuguuau      480

gaagcaagua uuagcgauau ggcgagugau agcagauguc ccacacaagg ugaagcauac      540

uuagauaaac aaagugacac acaauaugua ugcaaaagaa cucuaguaga uagaggaugg      600

ggaaacgguu gcggauuauu uggcaaaggu agcuuaguca caugugcaaa auuugccugc      660

aguaagaaaa ugacaggaaa aaguauacaa ccagaaaauu uagaauauag aauaaugcuu      720

agcgugcacg gaucacaaca uucaggaaug auagugaaug auacaggaca ugaaacagac      780

gaaaauaggg caaaaguuga aauuacgcca aauaguccca gggcagaagc aaccuuggga      840

ggauuuggca guuuaggacu agauugugaa ccaagaaccg gauuagauuu cucugaucua      900

uauuauuuaa cgaugaauaa uaaacacugg uuaguacaca aagaaugguu ucaugauaua      960

ccauuaccgu ggcacgcagg agcagauaca ggaacaccuc auuggaauaa uaaagaagcu     1020

cuaguagaau uuaaagacgc ccacgcgaaa agacaaacag ucguaguauu agguagccag     1080

gaaggagccg uucacacagc auuagccggu gcuuuagaag cagaaaugga uggagcaaaa     1140

gguagacuaa gcaguggaca cuuaaaaugu agguuaaaaa uggacaaauu aagacuaaaa     1200

gguguaucau auaguuuaug uacagcggca uuuacguuca caaaaauucc cgcagaaaca     1260

uuacacggaa caguaacagu agaaguacaa uaugcaggaa ccgauggacc auguaaagua     1320

ccagcacaaa uggcaguaga uaugcaaaca uuaacaccug uaggaagacu uaucacagca     1380

aauccuguua uaacagaaag cacagaaaau ucaaaaauga uguuagaauu agacccacca     1440

uucggugaua guuacauagu aauaggagua ggcgagaaga aaauuacaca ucauuggcau     1500

agaucaggau ccacaauagg uaaagcguuc gaagcaacag ugagaggagc aaaaagaaug     1560

gcaguauuag gggauacugc cugggauuuu ggaucaguag ggggagcauu aaauagucua     1620

ggaaaaggua uacaccaaau uuuuggagcg gcuuuuaaau cucuauuugg ugggauguca     1680

ugguuuucac aaauacuaau aggcacacua cucauguggc uaggauuaaa cacgaaaaau     1740

ggaagcauau cauuaaugug ucuagcacua ggaggugucc uaauauuccu gucaacagca     1800

guaagugca                                                             1809


<210>  9958
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9958
augggaaaaa ggagcgcagg aucaaucaug ugguuagcau cauuagcagu aguaauugcc       60

ugcgcuggug cagcaguaac auugccaagu cauucuacaa gaaaacuaca gacaagaagc      120

caaacauggc uagaaucuag agaauacaca aaacauuuaa uaagaguuga aaacuggaua      180

uuuagaaauc caggauuugc acuagcagca gcagcaauag ccugguuauu aggaaguuca      240

acaagucaaa aaguaauuua cuuaguaaug auacuauuaa uagcuccagc auauaguaua      300

agauguauag gaguaucaaa uagagauuuu guugaaggua ugaguggagg aacaugggua      360

gauguuguac uagaacaugg aggaugugua acuguaaugg cacaagauaa accaacaguc      420

gacauugaac uaguaacaac uacagugucu aacauggcug aaguaagauc auauugcuau      480

gaagcaucua uaagugauau ggcaucagau agcagauguc cuacucaagg agaagcauau      540

cuugauaaac aaucagauac acaauaugua uguaaaagaa cauuaguaga uagaggaugg      600

ggaaaugguu guggcuuauu uggaaaagga agucuaguaa caugugcaaa auuugcaugc      660

aguaaaaaaa ugacgggaaa aaguauacag ccagaaaacc uagaauacag aauuauguua      720

agcguucaug guagucaaca uaguggaaug auaguaaacg auacugggca ugaaacugac      780

gagaauagag ccaaaguaga aauaacacca aauucaccga gagcagaagc uacacuuggu      840

ggauucggau cacuuggucu agauugcgaa ccaagaacag gacuugacuu cucagaccua      900

uauuaucuaa caaugaauaa uaaacauugg uuaguucaua aagaaugguu ucaugauaua      960

ccauuaccau ggcaugcagg agcagauacg ggaacaccac auuggaauaa uaaagaagca     1020

cuagucgaau uuaaagaugc acaugcaaaa agacaaacgg uaguuguacu aggaucacaa     1080

gaaggagcag uacauacagc auuagcuggg gcuuuggaag cagaaaugga uggcgcgaaa     1140

ggaagacuau caaguggaca uuuaaaaugc cgauugaaaa uggauaaauu aagauuaaaa     1200

ggaguaaguu acucauuaug uaccgcugcg uuuacauuua cgaaaauucc agcagaaacg     1260

uuacauggaa caguaacagu cgaaguucaa uaugcaggaa cagacggacc auguaaagug     1320

ccagcacaaa uggcaguaga caugcaaaca uuaacaccag ugggaagauu aauaacagca     1380

aacccaguaa uaacagaauc uacggaaaau agcaaaauga uguuagaacu agacccaccc     1440

uucggagauu cauauauugu aauaggaguu ggagaaaaaa aaauuacgca ucacuggcau     1500

agaaguggua guacaauugg aaaagcauuu gaagcuacag uaaggggagc aaaaagaaug     1560

gcaguacuag gagauacugc augggauuuc gguaguguug gaggugcacu aaauagccuu     1620

ggaaaaggaa uucaucagau auuuggagca gcauuuaaau cauuauuugg aggcaugagu     1680

ugguucaguc aaauauuaau uggaacucua uuaauguggu uagguuuaaa cgcaaagaau     1740

ggaucaauaa guuuaaugug uuuagcacua ggaggaguau uaauauuccu uucaacagcc     1800

guuucggcu                                                             1809


<210>  9959
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9959
augggaaaaa gaucagcagg aaguauaaug ugguuagcau cacuagcagu aguaauagca       60

ugugcuggag cagcaguaac auuaccauca cauucaacaa gaaaauuaca gacaagaagc      120

caaaccuggu uagaaucaag agaauacaca aaacauuuaa uaaaagucga aaacuggaua      180

uuuagaaauc caggauucgc ucuaguagca guagcaauag cuugguuacu aggaucaagc      240

acaucacaaa aagugaucua ucuaguuaug auacuauuaa uagccccugc auauucuaua      300

aggugcauag gaguaagcaa cagagauuuu gucgaaggaa ugucaggagg aacaugggua      360

gauguugucu uagaacaugg agguugcguu acaguaaugg cacaagauaa accaaccgug      420

gauauugagc uaguaacgac aacaguaagu aacauggcag aaguaagaag uuauuguuac      480

gaagcaagua uaucagacau ggcaucagau ucaagauguc cgacucaagg agaagcauau      540

cuagauaagc aaucagauac acaauacguu ugcaaaagaa cauuaguaga uagaggaugg      600

ggaaauggau gcggauuauu uggaaaagga aguuuaguaa caugcgcaaa auuuacuugu      660

aguaagaaaa ugacaggaaa aaguauucaa ccugaaaauc ucgaauauag aaucaugcua      720

aguguucaug gaagccaaca uucuggcaug auaggauaug aaacagauga agauagggca      780

aaaguagaag uuacaccuaa uucaccaaga gcagaagcaa cauuaggagg uuuuggaagu      840

uugggacuag acugugaacc ucgaacuggu uuagacuuua gugauuuaua cuauuuaacg      900

augaauaaua aacauuggcu uguacauaaa gaaugguuuc augacauacc auuaccaugg      960

cacgcaggag cagauacagg aacaccacac uggaauaaua aagaagcauu aguagaguuu     1020

aaagaugcuc augcaaaacg acaaacagug guaguauuag gaucucaaga aggagcugua     1080

cauacagcuc uggcgggugc auuggaagca gaaauggaug gagcaaaagg acgacuguuc     1140

agcggacauu uaaaaugcag gcuuaaaaug gauaaauuaa gauuaaaagg aguaucauau     1200

ucacuaugua cagcugcuuu uacauucaca aaagucccag cagagacucu acauggaaca     1260

guaacuguug aaguacaaua ugccggaaca gauggaccau guaaaauucc uguacaaaug     1320

gcaguagaua ugcaaacacu uacuccagua ggaagauuaa uaacggcaaa ccccguuaua     1380

accgaaucaa cagaaaauag uaaaaugaug cuagaacuag auccuccauu uggugauuca     1440

uauauaguaa uaggaguagg agacaagaaa auaacacacc auuggcauag aaguggaagu     1500

acuauaggaa aagcauuuga agcaacagug agaggagcca aaagaauggc uguacuagga     1560

gauacagcau gggauuucgg uucaguaggc ggaguuuuua acucauuagg aaaagggaua     1620

caucaaauau uuggagcagc cuucaaauca cuguucggcg gaaugagcug guuuucucaa     1680

auauugauug ggacccuauu gguauggcuc ggacuaaaua cuaaaaaugg uagcauauca     1740

uuaacaugcc uagccuuagg aggaguaaug auuuuccuua gcacagcagu uagcgca        1797


<210>  9960
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9960
augggcaaaa gaucagcagg aagcaucaug ugguuagcua guuuagcagu cguaauagcc       60

ugugcaggag cagcaguaac auuaccgagc cacucaacaa ggaaauugca aacccggagc      120

caaacauggc uagaaaguag agaauauaca aaacaucuaa ucagagucga aaacuggaua      180

uucagaaauc cuggauuugc auuagcagca gcagcaauag cauggcuauu aggaucaagu      240

acaagucaaa aaguaauaua ucuaguuaug auauuacuaa uagcaccagc cuacucaaua      300

agauguauag gaguaagcaa uagagacuuu gucgaaggaa ugagcggagg aacuugggua      360

gauguaguau uagaacacgg agguugcgua acggucaugg cacaagacaa accaacagua      420

gacauugaau uaguaacaac gacaguuuca aauauggcag aaguccgaag uuacuguuau      480

gaagcgucaa uaucugacau ggcaagugac ucuagauguc cuacucaagg agaggcuuau      540

uuagauaaac aaagugauac acaauaugua uguaaacgaa cguuagugga uagaggaugg      600

gguaaugguu gcggucuguu cgguaaagga ucucuaguaa caugugcaaa auuugcuugu      660

uccaagaaaa ugacaggaaa aucaauacaa ccagaaaauu uagaauaucg aauaauguua      720

aguguacaug gaagucaaca uaguggaaug auuguaaaug auacaggaca ugaaacagau      780

gaaaauagag caaaagugga aauaacucca aauucaccaa gagcagaagc uacauuagga      840

ggcuuuggua gcuuaggauu agacugcgaa ccaaggacgg gucuagacuu uagugaccuu      900

uauuaucuaa caaugaauaa uaaacauugg uuaguacaua aagaaugguu ccaugauauu      960

ccauuaccau ggcacgcagg agcagauacu ggaacaccac auuggaacaa uaaagaagca     1020

uugguugaau uuaaagaugc acaugccaaa agacaaacag uaguaguacu uggaucccaa     1080

gaaggagcag uacauacagc auuagccggu gcauuagaag cagaaaugga cggagcaaaa     1140

gguagacuua gcagcggaca ucuuaaaugu agacuaaaaa uggauaaauu aagauuaaaa     1200

ggaguaucuu auagucuaug cacagcagca uucacuuuua cuaaaauacc cgcagaaacg     1260

uuacauggaa caguaacagu agaaguacaa uaugcaggaa cagauggccc auguaaagua     1320

ccagcacaaa uggcaguuga uaugcagaca uuaacaccug uaggaagacu aauaacagca     1380

aauccaguaa uaacagaauc aacagaaaau aguaaaauga uguuggaauu agauccacca     1440

uucggagauu cuuacauagu aauaggagua ggagaaaaga aaauaaccca ucauuggcau     1500

agaucaggaa guacaauugg aaaagcauuu gaagcaacag uaagaggagc aaaacgaaug     1560

gcaguacuug gagacacagc augggauuuu gguucgguag gaggagcauu aaauucacua     1620

gguaaaggaa uacaucaaau auuuggagca gcguuuaaau cacucuucgg uggaauguca     1680

ugguucaguc aaauucuaau aggaacauua uuaauguggc ucggucuaaa uacgaaaaau     1740

ggaucaauuu cacucaugug uuuagccuua gguggagucc ucauauucuu aucaacagca     1800

gucagcgca                                                             1809


<210>  9961
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9961
auggccauaa gcggcguacc cguccucgga uucuucauaa ucgcaguccu caugagcgcc       60

caagaaaguu gggccgucac ccgcagagga agugcauacu acauguaccu cgaccgcaac      120

gacgcgggag aagccaucuc cuucccaacc acccuaggca ugaacaaaug cuacauccaa      180

aucauggacc uaggacauau gugcgacgcc acaaugagcu acgaaugccc caugcucgac      240

gaagguguag aacccgacga cguagacugc uggugcaaca caacaucaac cuggguagua      300

uacggaacau gccaucacaa aaagggagaa gccagacgca gccgcagagc cguaacacua      360

cccucacacu cgacacgaaa acuucaaaca cggucacaga ccuggcuaga aucccgugaa      420

uacaccaaac accuuauaag aguagagaac uggaucuucc gaaaccccgg guucgcccuc      480

gccgccgccg ccaucgcaug gcuacucggc uccagcacau cacaaaaagu uauauaccua      540

guuaugaucc uccugauagc cccugccuau agcauaagau guauaggcgu aucaaaccgc      600

gacuucguag aaggcauguc aggcggaaca uggguggaca uaguccuaga acacggcggc      660

ugcgucaccg uaauggccca agacaaacca accguagaca uagaacuugu aaccaccacg      720

gugucaaaca uggccgaagu acggagcuac ugcuacgaag ccucaauuuc cgacauggcg      780

agcgacucca ggugcccgac acagggagaa gcauaccucg acaagcaauc agacacccag      840

uacgucugca agagaacacu cguagacaga ggcuggggaa acgguugcgg acuguucggc      900

aaaggcagcc uggucacaug cgcgaaauuc gccuguagca aaaagaugac cggaaagucg      960

auccaacccg aaaaccuaga auacaggauc augcugagcg uccauggcag ccaacacagc     1020

ggcaugaucg uaaacgacac cggacacgaa accgacgaaa accgggcuaa aguagaaauc     1080

accccaaacu cccccagagc cgaagccacg cuagggggau ucggcucacu ugggcucgac     1140

ugcgaaccca gaaccggacu agauuucagc gaucuauacu acuuaaccau gaacaacaaa     1200

cacuggcucg uacacaaaga gugguuccac gacauacccc ugccauggca cgccggcgcc     1260

gacaccggca caccacacug gaacaacaaa gaagcgcuug uagaguucaa ggacgcacac     1320

gcuaaacgcc aaaccguagu agugcugggg agccaagaag gcgcgguaca cacggcccuc     1380

gccggggcac uagaagcaga aauggacgga gccaaggggc ggcugucaag cggccaccug     1440

aaaugccgcc ucaaaaugga caaacuuagg cuuaaaggag ucuccuauag ccuaugcacg     1500

gccgccuuca ccuucacaaa aauaccagcc gaaacccucc acggcacugu aacagucgag     1560

guccaauacg ccggcacaga cggacccugu aaaguaccag cgcagauggc cguagacaug     1620

caaacgcuua cacccguagg acgccuaauc acagcaaacc caguaaucac cgaguccaca     1680

gaaaacagca aaaugaugcu ugaacucgau cccccauucg gggacuccua cauagucauc     1740

ggcgucggcg aaaagaaaau aacccaccac uggcaccgcu ccggcucaac gauaggaaag     1800

gcauucgaag caacaguaag aggagcaaaa agaauggcag uccucgggga cacagccugg     1860

gacuucggau cgguaggcgg cgcccucaac agccucggaa agggcauaca ccaaaucuuc     1920

ggagcagccu ucaaaucacu auucggaggc augagcuggu ucagucaaau ccugaucggc     1980

acccuccuca uguggcucgg ccucaacacg aaaaacggau ccaucucccu caugugccua     2040

gcacucggcg gaguccucau auuccuuucc accgcgguga gcgcg                     2085


<210>  9962
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9962
auggcaauau cgggaguacc cguacuggga uucuucauaa uagcaguccu cauguccgca       60

caagaauccu gggcagucac aagacgcgga uccgccuacu acauguaccu agaccgaaac      120

gacgccggcg aagccaucuc auucccaaca acacuaggaa ugaacaaaug cuacauccaa      180

aucauggacc ucggacauac gugcgacgca acaaugagcu acgaaugccc caugcuggac      240

gaaggaguag aaccagacga cgucgacugc uggugcaaca caaccagcac uuggguagua      300

uacggcacau gccaccacaa gaaaggcgaa gccagacgaa gcagaagggc aguaacccua      360

ccuucgcacu cgacccgaaa acugcagaca agaagccaga cauggcugga aucacgcgaa      420

uacacaaaac accucauacg agucgagaac uggaucuuca ggaaccccgg auuugcccug      480

gcggccgccg caaucgcaug gcuccucggc agcucaacgu cacaaaaagu aauauaccug      540

gucaugaucc uacucaucgc acccgccuac agcaucagau gcauaggagu cagcaaccgg      600

gacuucguag aaggcaugag cggcggcacc uggguagaug ugguucuaga acacggcggu      660

ugcgucacag ugauggcaca agacaaaccc accguagaua uagagcuagu caccaccacc      720

gucagcaaua uggccgaagu ccggagcuac ugcuacgaag ccagcaucag cgacauggca      780

uccgacagcc gcugcccaac ccaaggagag gcauaccuag acaaacaaag ugacacacaa      840

uacgucugca aacgaacacu cguagacaga ggcuggggca acggaugugg ccucuucggu      900

aaaggcucac ucgucacaug cgcaaaauuc gcaugcagca agaaaaugac cggcaaaagc      960

auccaaccag aaaaccuuga guauagaauc augcuaucgg uacacggauc ccaacauuca     1020

ggcaugaucg uaaacgacac gggccaugaa acagacgaaa aucgagccaa aguagaaaua     1080

accccgaaca gcccgagggc agaggcaaca cucgguggau uuggcagccu gggccucgac     1140

ugcgaacccc gaaccggucu ugacuucucc gaccuguacu accuaacaau gaacaacaaa     1200

cacuggcucg ugcauaaaga gugguuccac gacaucccac ugcccuggca cgccggcgca     1260

gacaccggua cgccacacug gaacaacaaa gaagcacucg ucgaguucaa agacgcacac     1320

gcaaaacgcc aaacgguagu aguccucggc ucacaagaag gagccgugca caccgcccua     1380

gccggcgcac ucgaagcaga aauggacggc gcaaaaggcc gacucuccuc aggccaccuc     1440

aaaugccgcc uaaaaaugga caaacugcgc cucaaaggag ucagcuacuc cuuaugcaca     1500

gcagccuuca ccuucacaaa aauaccugca gaaacccuuc acggcaccgu caccgucgaa     1560

guacaauacg cagguaccga cggccccugc aaaguaccag cccaaauggc cguagacaug     1620

caaacccuga cccccguggg cagacuuaua accgccaacc ccguaauaac agaaagcacc     1680

gaaaacucaa aaaugaugcu cgaacucgau cccccguucg gcgacagcua uauagucauc     1740

ggagucggcg aaaagaaaau cacacaucac uggcacagau caggaagcac caucggaaaa     1800

gccuucgagg cgacaguacg cggagccaaa cggauggccg uccucggaga caccgccugg     1860

gacuucggau caguaggagg ggcacuuaac ucccuaggaa aaggcaucca ccaaaucuuc     1920

ggcgcagccu ucaaaucacu auucgggggu auguccuggu ucucccaaau ccuaaucgga     1980

acucuccuca uguggcucgg acucaacgcg aaaaacggca guaucagccu caugugccuc     2040

gcacuaggag gagugcuaau auuccuauca acagcaguca gcgcc                     2085


<210>  9963
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9963
auggccaucu caggcguccc agugcucgga uuuuucauca uagcaguauu aaugagcgcg       60

caagaaaguu gggcaauaac cagacgcggc uccgccuacu acauguaccu ggaccgcucc      120

gacgcaggca aagccaucag cuucgcaacc acccucggag uaaauaagug ccacgugcaa      180

auaauggacc uaggccacau gugcgacgcc acaaugucau acgaaugccc gaugcucgac      240

gaaggaguag aacccgacga cguggacugc uggugcaaca caacguccac auggguagua      300

uacggaaccu gccaccacaa gaaaggagag gcaaggcgaa gccgcagagc aguaacccua      360

cccagccaca guacacgaaa acuacaaacg cggagccaaa cuuggcugga aucacgcgaa      420

uacacaaaac accuaauaaa aguggaaaac uggauauucc ggaaccccgg auucgcacug      480

guggcaguag cgauagccug gcugcucggc agcucaaccu cccaaaaagu caucuaccuc      540

guaaugauac uccugauagc accagcguac uccauaaggu gcauaggcgu cagcaaccgu      600

gauuucgucg agggaauguc aggaggaacc uggguagacg uaguccuaga acacgggggc      660

ugcgugaccg ucauggccca ggacaagccu acagucgaca uagaacucgu cacaaccacg      720

guaagcaaua uggcagaagu aagaagcuac uguuacgaag caucaaucag cgacauggcc      780

agcgacucaa gaugcccaac ccaaggagaa gccuaccuag acaaacagag cgacacccaa      840

uacgucugca agcggacgcu cguagacaga ggcuggggaa auggaugcgg ccucuucgga      900

aagggaagcc uagugaccug cgccaaauuc acaugcagca agaaaaugac aggaaaaucc      960

auccaacccg agaaccucga auaccgcaua auguuaagcg uccacggguc acaacauagc     1020

gguaugaucg gcuacgaaac cgacgaagac agggcaaaag uagaaguaac ccccaacagc     1080

ccaagagcug aagccacacu cggaggauuc ggcagccuag gacuagacug cgaaccacgc     1140

acuggacuug acuucuccga ccuauacuac cucacgauga acaacaagca cuggcuaguc     1200

cacaaagaau gguuccacga cauaccccua ccuuggcacg ccggcgcgga caccggaaca     1260

ccacacugga acaacaaaga ggcacucguc gaguucaagg augcacaugc caaacgccaa     1320

acagucguag uacugggaag ccaggaagga gccguacaca cagcccucgc aggagcacuc     1380

gaagcugaaa uggacggcgc aaaaggccgg cuuuucagcg gccaccucaa augccggcua     1440

aaaauggaca aacuccggcu gaaaggggua agcuacagcc ucugcaccgc agcguuuacg     1500

uucaccaaag uacccgcaga aacacuacac ggcacaguaa cagucgaggu gcaauacgca     1560

ggcaccgacg gccccugcaa gauaccagua caaauggcag ucgacaugca aacacugaca     1620

ccugucgggc gacuaauaac cgcaaaccca gucaucaccg aaagcaccga gaacagcaaa     1680

augaugcucg agcuagaccc accauucggc gacagcuaca ucgucauagg cgugggcgac     1740

aagaaaauca cacaccacug gcacaggucc ggcagcacca ucggcaaagc cuucgaagcg     1800

acaguacgcg gagcaaaacg aauggcaguc uugggcgaca cagccuggga cuucggcagc     1860

gucggcggag ucuucaacuc acuagggaaa ggaauacacc agauauucgg agccgccuuc     1920

aagagccuau ucggaggaau gagcugguuu agccagauac ucauaggcac ccuacucguc     1980

uggcuagguc uaaacacaaa gaacggcucc aucucauuaa caugccuggc auugggcgga     2040

gugaugauau uucucagcac agccguaagc gcg                                  2073


<210>  9964
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9964
auggccauau ccggcguacc aguccucggg uucuucauua uagcaguccu caugagcgca       60

caagaaucgu gggccguaac cagacgagga ucugcauauu acauguaccu agaccggaac      120

gacgcgggag aagccaucuc auuucccaca acacucggaa ugaauaaaug cuacauccaa      180

aucauggacc ucggccacau gugcgacgca acgaugagcu acgaaugccc caugcuagac      240

gagggggucg aacccgacga cgucgacugc uggugcaaca caacaucaac cuggguagua      300

uacggcacau gccaccacaa aaagggcgaa gcccgacgau ccaggcgagc aguaacauua      360

cccucccauu ccacccgcaa acuccaaacg cgcucgcaaa ccuggcuaga aucacgagaa      420

uacacaaaac accucauccg aguagaaaac uggaucuuca gaaauccggg auucgcccug      480

gccgcugccg ccaucgccug gcuacucggc uccagcaccu cacagaaagu caucuaccuc      540

guuaugauac uccucauagc gccagccuac ucuauccgcu gcaucggagu aagcaaccga      600

gauuucguag aaggcauguc aggcggcaca ugggucgacg ucguccuaga acacggaggc      660

ugcguaacag ucauggccca agacaaaccg accgucgaca ucgagcuagu aaccacaacc      720

gucuccaaua uggccgaagu ccgaagcuac ugcuacgaag cgucaaucuc cgacauggca      780

agcgacuccc gaugcccaac ccaaggcgaa gccuaccuag acaaacaguc cgacacccaa      840

uacguuugca aacgcacacu cgucgaccga ggauggggca acggcugcgg ccucuucggg      900

aaaggaagcc ucguaaccug cgcaaaauuc gcaugcagca agaaaaugac cggaaaaucc      960

auccaacccg aaaaccugga auaccgcaua augcucucag uacacggauc ccagcacagc     1020

ggaaugauag ugaacgauac aggacacgaa acugacgaaa accgagcaaa aguagaaauc     1080

acccccaacu caccacgagc agaagccacc cuaggcggau ucggcucauu aggccucgac     1140

ugcgaaccua ggacgggccu cgacuucucc gaccuauacu accugacaau gaacaacaaa     1200

cacuggcuag uacacaaaga augguuucac gauauccccc uacccuggca cgccggcgcc     1260

gacacaggca caccgcacug gaacaacaag gaagccuuag uagaguucaa agacgcccac     1320

gccaagcgcc aaaccgucgu gguccuggga ucccaagaag gcgccguaca caccgcccua     1380

gcaggcgccc uugaggccga aauggacggg gcaaaaggcc gccuaucauc cggacaucuc     1440

aagugccggc ucaaaaugga caaacuacga cuaaaaggcg uaagcuacuc ccucugcaca     1500

gccgcuuuca cauucaccaa aauaccagcg gaaacucucc acggcaccgu caccgucgaa     1560

gugcaauacg ccggaacaga cggaccaugc aaagugcccg cccaaauggc cgucgacaug     1620

cagacucuaa ccccaguagg gcgacucaua accgcaaauc ccgucaucac cgaaagcacc     1680

gagaacagca aaaugaugcu cgaacuggac ccucccuucg gcgacagcua caucgucauc     1740

ggaguaggcg aaaagaaaau cacccaccac uggcaccgau cgggaagcac caucggcaaa     1800

gccuucgaag cgacagucag aggcgcaaaa cgcauggccg uccucggaga caccgcaugg     1860

gacuucggca gcgucggagg cgcacucaac ucucugggca aagggaucca ccaaauauuc     1920

ggcgccgccu ucaaaucccu cuucggggga augucguggu ucucacagau acuuauagga     1980

acacuacuca uguggcucgg acuaaacacc aaaaacggcu ccauaucccu caugugccua     2040

gcccuaggag gaguccuaau cuuccucucg acagcggucu ccgcc                     2085


<210>  9965
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9965
auggacugga cauggauacu cuuccuagua gccgccgcca cccgcguaca cucagugacg       60

cgccgcggcu cagcauacua cauguaccuc gaccggaacg acgccggaga ggcgauauca      120

uuccccacca cacuaggaau gaacaaaugc uacauacaga ucauggaccu cggccacaug      180

ugcgacgcca caauguccua cgagugccca augcuggacg aagggguaga accagacgac      240

gucgacugcu ggugcaacac cacguccaca uggguggucu acggcacaug ccaccacaag      300

aaaggagaag cgcgccgauc acgcagagcc gucacccucc caagccacuc cacaagaaaa      360

cuccaaacca gaagccaaac uuggcucgaa ucccgggaau acaccaagca ccucauacgc      420

guagagaacu ggauauuccg caacccaggc uucgcgcuag cagccgccgc cauagccugg      480

cuacuaggcu cauccacauc acaaaaagug aucuaucuag uaaugauacu acuaauagca      540

cccgccuaca gcaucagaug cauaggcguc ucaaacagag acuucguaga aggcauguca      600

ggaggaaccu ggguagauau cguacucgaa cacggcggau guguaaccgu cauggcccaa      660

gacaaaccca cuguagauau cgagcuagua acaacaacag uauccaacau ggccgaagua      720

agauccuacu gcuacgaagc aucaaucuca gacauggccu cagacucgag gugcccuacu      780

cagggagaag ccuaccuaga caaacaaagc gacacgcaau acgucugcaa aagaacccua      840

gucgauagag gcuggggaaa cgggugcggg cuauucggca agggaucacu cguaaccugc      900

gcaaaguucg caugcuccaa gaaaaugacc gguaaaagca uccaacccga aaaccucgaa      960

uacagaauca ugcuaucggu acacggaucc cagcacuccg gaaugauugu uaacgacacg     1020

ggucacgaaa cagacgaaaa ccgggccaag gucgaaauaa caccaaacag cccacgagca     1080

gaagccaccc uaggaggcuu cggcucccuc ggccucgacu gcgaaccccg uaccggcuug     1140

gacuucagcg accuauacua ccucaccaug aacaacaaac acuggcuggu ccacaaagaa     1200

ugguuccacg acauuccccu ccccuggcac gcaggggccg acacaggaac cccacacugg     1260

aacaacaaag aggcccuugu agaguucaaa gacgcacacg caaaacgcca aacagucguc     1320

guccucggcu cgcaagaagg agccguacac acagcacuag ccggcgcacu agaagcagaa     1380

auggacggcg caaaaggccg ccuaagcuca ggccaccuca agugccggcu caagauggac     1440

aaacuacgcc uaaaaggagu cucauacucc cuaugcaccg cggccuucac cuucaccaaa     1500

aucccggcag agacccuaca cggaaccgua accguugaag uucaauaugc cggaaccgac     1560

ggacccugca aaguacccgc ccagauggca gucgacaugc aaacacuuac cccaguuggc     1620

cgccucauca cggcaaaucc cgucaucacc gaaaguacag aaaacucaaa aaugaugcua     1680

gaacuagacc cgcccuuugg cgacucauac auagucauag ggguaggaga gaaaaagaua     1740

acccaccacu ggcaccgcag cggguccacg auagguaaag ccuucgaagc aaccguaaga     1800

ggcgcgaaac gaauggcagu acuaggugac accgcauggg acuucgggag cgucggcggg     1860

gcacuaaauu cacuugggaa gggaauccac caaaucuucg gggccgcauu caagagucuc     1920

uucggcggca ugagcugguu cucacaaauc cucauaggaa cccuccuaau guggcuaggc     1980

cucaacacca aaaacggaag cauaucccua augugccuag cacucggagg cguacucauc     2040

uuccucagca cagcgguaag cgcg                                            2064


<210>  9966
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9966
auggacugga cguggauacu auuccucgua gccgccgcaa cccgcgucca cagcguaacc       60

cgaagaggcu ccgcuuacua cauguaccuc gaccgaaacg acgccggcga agccauauca      120

uuccccacca cccucggcau gaacaaaugc uacauccaaa uuauggaccu agggcacacu      180

ugcgacgcca caaugagcua cgagugcccc augcucgacg aaggcgucga accagacgac      240

guggacugcu ggugcaacac caccagcaca uggguaguau acggaaccug ccaccacaag      300

aaaggcgaag cccgaagauc acgccgggcc guuacccucc ccucacacuc cacacgaaag      360

cuccaaaccc gcucccaaac cuggcucgaa ucucgagaau acacaaagca ccuuauaaga      420

guugagaacu ggaucuucag aaaccccggc uucgcccuag ccgcagccgc aaucgccugg      480

cuccucggcu ccucaacauc ccaaaaaguc auauaccuug ucaugauccu ccucauagca      540

ccagccuacu cgauccgaug caucggcguu uccaaccgcg acuuugucga aggcaugagu      600

ggaggcaccu gggucgacgu aguacuagaa cacggaggcu gcgucacagu aauggcacaa      660

gacaaaccga caguagacau cgagcuaguu acaacaaccg uaucuaauau ggccgaaguc      720

cgcucauacu gcuacgaagc cuccaucucc gacauggcau ccgacuccag augcccuacc      780

caaggcgaag ccuaccucga caaacaaucc gacacccaau acgucugcaa acgcacccuc      840

guagaccgag gguggggcaa cgguugcggc cuguucggaa agggaucccu agucacaugc      900

gcaaaguuug ccugcuccaa gaaaaugacg ggaaaaucaa uacaacccga aaaccuagaa      960

uaccgcauaa ugcucagcgu ccacggguca caacacagcg guaugauagu gaacgacacu     1020

ggacacgaaa cagacgagaa cagagcgaaa gucgaaauca caccgaacuc cccucgcgca     1080

gaagccacgc uaggaggguu cggaucacuu ggacucgacu gcgaaccccg caccggccuc     1140

gacuucagcg acuuauacua ccuaacaaug aacaacaaac auuggcuagu ccacaaagaa     1200

ugguuccacg acauaccccu acccuggcac gccggcgcag acacaggaac accccacugg     1260

aacaauaaag aagcccucgu cgaguucaaa gacgcccacg cgaaaagaca aaccgucguu     1320

guacugggca gccaagaagg cgccguccac accgcacuag cgggggcucu agaagcagaa     1380

auggauggcg ccaaggggcg acuaucauca ggccaccuca aaugcagacu aaaaauggac     1440

aaacuccgcc ucaaaggagu aucauacuca cuaugcaccg ccgcguucac auucaccaaa     1500

auacccgcug aaacucucca cggaacaguc accgucgaag uccaauacgc aggaaccgac     1560

ggccccugca agguaccagc ccaaauggcc guagacaugc aaacacuaac ccccgucgga     1620

agacuaauca ccgcgaaccc cguaauaaca gaauccacag agaacucaaa aaugaugcuc     1680

gaacuagacc caccguucgg agacucuuac auagucauag gagucggaga gaaaaagauc     1740

acacaccacu ggcaccgcuc aggcucaaca auuggcaaag ccuucgaagc cacgguacgg     1800

ggagccaaaa gaauggccgu acuaggggac acagccuggg acuucggauc agucggcggu     1860

gcacuaaauu cacuaggaaa aggcauccac cagaucuucg gcgcagccuu caaaagccua     1920

uuugguggaa ugucaugguu cagccaaauc cucauaggga cccuccucau guggcucggc     1980

cucaacgcua aaaacggaag caucucgcua augugccuag cccucggggg cguccuaaua     2040

uuccucagca cggcagucag cgcc                                            2064


<210>  9967
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9967
auggacugga ccuggauacu guucuuagua gccgccgcca cgcgagucca cagcauaaca       60

cgcaggggau cagcauacua cauguaccuc gaccgauccg acgccggaaa agcaauauca      120

uucgcaacca cgcuaggagu caacaaaugc cacgugcaaa ucauggaccu aggucacaug      180

ugcgacgcca ccauguccua cgagugcccu augcucgacg aaggaguaga accagaugac      240

gucgacugcu gguguaacac aaccuccaca ugggucgucu acggcacaug ccaccacaag      300

aaaggagaag ccagacgaag ccgucgcgcc gucacacuac caagccacag cacaagaaaa      360

cuccaaaccc ggucacaaac auggcuugaa agccgagaau auacaaaaca ccucauaaaa      420

guagaaaacu ggaucuuccg gaacccaggg uucgcgcucg uagccguggc aauagccugg      480

cuccucggau ccagcacauc ccaaaaaguc aucuaccuag uaaugauccu cuugauagca      540

cccgccuacu caauacgaug cauaggaguc agcaaccgcg auuucguaga aggaaugucc      600

ggcggaacau gggucgacgu cguccucgaa cacgguggau gcgucaccgu cauggcacaa      660

gacaaaccca cagucgacau agagcuaguc accacaacug uaagcaacau ggccgaagua      720

cgcuccuacu gcuaugaagc auccauaucc gacauggcau cggacagccg cugcccaacg      780

caaggcgaag ccuaccuaga caaacaauca gauacccagu acgucugcaa aaggacccua      840

guagaccgag gauggggcaa cggaugcgga cucuucggaa aaggcagccu aguaaccugc      900

gcaaaauuca caugcuccaa gaaaaugaca ggaaaaagca uacaaccgga aaaccuagag      960

uacaggauca ugcuaagcgu gcacggcucc caacacucag gaaugauagg cuacgaaacc     1020

gacgaagacc gagcaaaagu cgaaguaaca cccaacucac cccgggcaga agcaacccua     1080

gggggauucg gaucccuagg acucgacugc gaaccccgca caggacugga cuucuccgac     1140

cuauacuacc uaacgaugaa caacaaacac ugguuagucc acaaagagug guuccacgau     1200

aucccccuac cauggcacgc cggagccgac acaggaacgc cacacuggaa caacaaagaa     1260

gcccuagucg aguucaaaga cgcacacgcc aaaaggcaga cagucguagu gcuaggcucg     1320

caagaagggg caguccacac cgcacucgcc ggagcacuag aagccgaaau ggacggagcc     1380

aaggggcggc uauucucagg ccaccugaaa uguagacuua agauggauaa acugcgacua     1440

aaaggaguga gcuacucccu cugcacagcc gcauucacgu ucacaaaagu accggccgaa     1500

acacugcacg ggacagucac cguugaaguc caauacgcag ggacugacgg ccccugcaaa     1560

auccccgucc aaauggccgu cgacaugcaa acccuaaccc ccguaggacg acuaaucacg     1620

gcaaacccug uaauaacaga aucaacagaa aacagcaaaa ugaugcuuga acuagaccca     1680

ccauucggag acuccuacau cguaaucgga guaggugaca agaagauaac acaccacugg     1740

cacagaagcg gcuccacaau cgggaaagcg uucgaagcca caguccgcgg cgccaaaaga     1800

auggcuguac uaggagacac cgcaugggac uucggcuccg uaggcggagu cuucaacucg     1860

cuagggaaag gaauacacca aauauucgga gcagccuuca aaucccuauu cggaggaaug     1920

uccugguucu cgcaaauccu aaucggcaca cuccuggucu ggcuaggacu aaacacgaaa     1980

aauggaagca uuucccuaac augccucgcc cucggaggag uaaugauauu ccucagcacc     2040

gccguaagcg cc                                                         2052


<210>  9968
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9968
auggacugga ccuggauacu auuccucgua gccgccgcaa cacgagucca cagcguaacc       60

aggcgcggau cagccuacua cauguaccug gaccgaaacg augccggcga ggcgauauca      120

uuccccacaa cccucggcau gaacaaaugc uacauccaga ucauggaccu cgggcauaug      180

ugcgacgcca ccauguccua ugaaugcccg augcucgaug aaggagucga accagacgac      240

gucgacugcu ggugcaacac caccagcacc ugggucguau acggaaccug ccaccacaag      300

aaaggcgaag cccgccgaag cagacgcgca gucacacuac caagccacuc aacacgaaaa      360

cuacaaaccc gaucacaaac cuggcucgaa ucccgagaau acacaaaaca ccuaauccga      420

gucgaaaacu ggauauuccg aaacccagga uucgcccucg cggccgccgc cauagccugg      480

cuacugggau ccagcacaag ccaaaaaguc aucuaccuug ucaugauccu acuaaucgcc      540

ccagccuacu ccauacgaug caucggaguc agcaacagag acuucgucga aggcauguca      600

ggaggaacau ggguagacgu aguacucgaa cacggcggcu gcguaaccgu aauggcccaa      660

gacaaaccca caguagacau agaacuugua accaccacag ucuccaacau ggcagaaguc      720

cgcucauacu gcuacgaagc auccaucagc gacauggcaa gcgacucacg cugcccaacc      780

caaggcgaag cauaccugga caaacaaagc gacacgcaau acgucugcaa aagaacacuc      840

guggaccgcg gauggggcaa uggaugcggc cucuucggca aggggagccu agugaccugc      900

gcaaaauucg ccuguucaaa gaaaaugacc ggcaaaagca uacaaccuga gaaccucgaa      960

uaccgcauua ugcugagcgu ccacgggagc caacacagcg gcaugaucgu aaacgacacc     1020

ggucacgaaa ccgacgaaaa ccgcgccaaa gucgagauca ccccaaacuc accccgcgcc     1080

gaggcuacac ugggaggauu cggcucacua gggcuugacu gcgaaccgag aacaggcuua     1140

gacuuuucag accuuuacua cuuaaccaug aacaacaaac acuggcucgu ccacaaagaa     1200

ugguuucacg acaucccccu gccauggcac gccggcgcug acaccgguac accacacugg     1260

aacaacaaag aggcccuagu agaguucaaa gacgcccacg caaaacgcca aaccguaguc     1320

guacuaggcu cccaagaagg agcaguacac accgcccugg ccggagcacu agaagcagag     1380

auggacggag ccaaggggcg ccucagcucc gggcaccuca aaugucgccu gaaaauggac     1440

aagcuaaggc uaaagggcgu auccuacucc cuaugcacag ccgccuucac cuucaccaaa     1500

aucccagcag aaacccucca cggaacggug acuguggaag uccaauacgc aggaaccgau     1560

ggacccugca aaguacccgc acagauggcg guggacaugc aaacccuaac gcccgucgga     1620

cgccucauaa ccgccaaccc agucauaaca gaaucaacag aaaacagcaa aaugaugcuc     1680

gagcucgacc cacccuucgg agacagcuac aucgucaucg ggguaggcga aaagaaaaua     1740

acccaccacu ggcacagauc aggaucuaca aucggaaaag ccuucgaagc caccguccgc     1800

ggugcgaaac gaauggccgu ccuaggcgac accgccuggg acuucggcag cgucgggggc     1860

gcacucaacu cccuaggaaa aggcauccac caaaucuucg gcgccgccuu caaaagccuc     1920

uucggaggca ugucaugguu cagccagauc cucauaggca cacuccuaau guggcuaggc     1980

cucaacacca aaaacggguc aauuagccua augugccucg cacugggagg cgugcuaauc     2040

uuccuaagca ccgcagucuc ggca                                            2064


<210>  9969
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9969
augggaaaaa gaagcgcagg aucaauaaug uggcuagcuu cccuggccgu aguaaucgcc       60

ugcgcaggcg caguaacacg ucgagggucc gcauacuaca uguaccucga uaggaacgac      120

gcaggagagg caaucucuuu ccccaccaca cuaggcauga acaaaugcua cauccaaauc      180

auggaccuag gacacaugug cgacgccacu auguccuacg aaugccccau gcuagacgaa      240

ggaguagaac cggacgacgu ugacugcugg ugcaacacca caucaaccug ggucgucuac      300

ggaacaugcc accacaagaa aggagaagcc agacgaagca gacgagccgu uacacuacca      360

ucccacagca caagaaaacu acaaaccaga ucacagaccu ggcuagaaag cagagaauac      420

accaaacauc uaauccgagu cgaaaacugg aucuucagaa accccggcuu cgcccuggca      480

gcagccgcaa uagccuggcu ccucggcagc aguacauccc aaaaagugau auaucucguc      540

augauacuau ugaucgcacc cgcauacagc aucagaugua uaggcgucag caaccgugac      600

uucguagagg gaaugagcgg cgggaccugg gucgacaucg uccuggaaca cggcggaugc      660

guaaccguua uggcacagga caaacccacc guagacaucg agcuaguaac aaccacaguc      720

uccaacaugg ccgaaguacg cuccuacugc uacgaagcgu ccauaagcga cauggcaagc      780

gacagccgcu gcccaaccca aggggaggca uaccuagaca aacaaagcga cacucaguac      840

gucuguaaaa gaacacucgu agacagagga uggggcaaug gaugcggccu auucggaaaa      900

ggcagccucg uaaccugcgc caaauucgca ugcaguaaga aaaugaccgg aaaaagcaua      960

caacccgaga aucuagagua ccggaucaug cucagcguac acggcuccca acacuccgga     1020

augauaguaa acgacacagg acacgaaaca gacgaaaacc gggccaaagu cgagaucaca     1080

ccaaacuccc caagggccga agccacccug ggcgguuucg gcagccuagg acuagacugc     1140

gaaccccgca ccggccucga cuucagcgac cuauacuacc uaaccaugaa caacaaacac     1200

ugguuagucc acaaagaaug guuccacgac auaccccucc cguggcacgc aggcgcagac     1260

accggcaccc cucacuggaa caacaaagaa gcacucgucg aguucaaaga cgcccacgcc     1320

aaaaggcaaa ccgucgucgu acucggguca caagaaggag ccguacacac cgcacuagcc     1380

ggagcccuag aagcagaaau ggacggcgca aaagggagac uauccuccgg ccaccucaaa     1440

ugccgacuga aaauggauaa acuacgacua aaaggcguca gcuacagccu gugcaccgca     1500

gcauucaccu ucaccaagau acccgccgaa acccuccacg gaacagucac cgucgaaguc     1560

caauaugcag gaacagacgg ccccugcaaa guccccgcac aaauggccgu ggacaugcaa     1620

acccuuacac caguaggaag acucaucaca gcaaacccgg ucaucaccga aagcacggaa     1680

aacucgaaga ugaugcucga gcuagacccg cccuucggag auagcuauau aguaauagga     1740

guuggagaaa agaaaauaac ccaccacugg cacagaagcg gguccacaau cggaaaagca     1800

uucgaagcga caguacgagg ggccaaacga auggcagucc ucggagacac agccugggac     1860

uucggauccg uaggaggagc ccuaaacucg cucggcaaag gcauacacca aaucuucggc     1920

gccgcguuca aaucccuauu cggaggcaug agcugguuca gccaaauacu aauagguacc     1980

cuacucaugu ggcucggacu gaacaccaaa aacggaagca ucagucuuau gugccuagca     2040

cucggaggcg uacuaauauu ccuaucaaca gccguaagcg cc                        2082


<210>  9970
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9970
augggcaaaa gguccgccgg aagcauaaug uggcuggcca gccuagcagu cgugaucgca       60

ugcgccggcg cgguaacacg ccggggcucg gcauacuaca uguaccucga ccgaaacgac      120

gccggggaag ccaucagcuu cccgaccacu cucgggauga acaagugcua cauacagaua      180

auggaccuug gacauaccug cgacgcaaca augagcuacg aaugcccaau guuagacgag      240

ggaguagaac cagacgacgu ggacugcugg ugcaacacaa cguccaccug gguagucuac      300

ggaaccugcc accacaagaa aggcgaggcg cgaagaagcc gccgagccgu cacacuaccc      360

agucacucaa cacggaaacu acaaacaaga ucacaaacau ggcuagagag cagggaauac      420

accaaacacc ucauccgagu cgagaacugg auauucagaa acccaggcuu cgcccucgcc      480

gccgcggcga uagccuggcu acucgggagc uccacaagcc agaaaguaau auaccuagug      540

augauacucc uaauagcgcc ugcauacucg auccgaugca uuggaguaag caaccgcgac      600

uucguggaag gcaugucagg cggaaccugg guagacguag uccuagagca cggaggcugc      660

guaaccguaa uggcccagga caaacccaca gucgacaucg agcuagucac gacaaccguc      720

agcaacaugg cagaaguacg uagcuacugc uacgaagcau caaucucaga cauggcauca      780

gacagucgau gccccacaca aggagaagcc uaccuagaca aacagucaga cacacaauac      840

gucugcaaaa ggacccucgu cgaccgaggc ugggggaacg ggugcggauu guucggcaaa      900

ggaucccucg uuacaugcgc aaaauuugca uguucgaaga aaaugacugg gaaaucaaua      960

caaccggaaa aucuggaaua cagaaucaug cuauccguac acggcagcca gcacagcggu     1020

augaucguca augacaccgg ccacgaaacu gacgaaaaca gagcaaaagu cgaaaucacg     1080

cccaacagcc cacgggcgga ggccacacuc ggaggcuucg gaucacuagg ccuagacugc     1140

gaaccacgca ccggccuaga cuucagugac cuauauuacc ugaccaugaa caacaaacac     1200

uggcucguac acaaagaaug guuccacgac auaccccuac cguggcacgc gggggccgac     1260

accgggacac cacacuggaa caauaaagaa gcccuaguag aguucaaaga cgcgcacgcc     1320

aaacgccaaa cagucguagu acucggcuca caagaaggag ccguccacac cgcacucgca     1380

ggagcucucg aagcagaaau ggacggagcc aaaggacgac uuagcagcgg acaccuaaaa     1440

ugcagacuca aaauggacaa acucagauua aaaggaguca gcuacucgcu augcacagca     1500

gcauucaccu ucacaaagau ccccgcggaa acccuacacg ggacggucac aguagaaguc     1560

caauacgccg gcacggacgg gccaugcaag guucccgcac aaauggcggu cgacaugcag     1620

acacugacac cagucggcag acuaauaacc gcgaaccccg uaauaacaga aagcacagag     1680

aacucgaaaa ugaugcugga acuagaccca ccguucggag acucauauau ugugaucgga     1740

guaggagaaa agaaaauaac acaccacugg caucgcagcg ggagcacaau cggaaaagca     1800

uucgaagcua caguccgcgg cgccaaaaga auggcagugc ucggcgacac ggccugggac     1860

uucggaucag uaggcggagc ccuaaacuca cuuggaaaag gaauacacca aauauucggc     1920

gcagcguuca aaagucucuu cggcgggaug ucaugguuca gccaaauccu caucggcacc     1980

cuacuaaugu ggcucggccu aaaugcaaag aacggcagca uaagccucau gugccuagcc     2040

cuaggaggag uacuaaucuu ccuaaguacc gccguaagcg ca                        2082


<210>  9971
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9971
augggcaagc gaucagccgg auccaucaug uggcucgcca gccuagcagu agucaucgca       60

ugcgcagggg ccaucacaag acgaggcucc gcguauuaca uguaccuaga ccguagcgac      120

gccggaaagg caaucagcuu cgcaaccacc cugggaguca acaaauguca cguacaaauc      180

auggaccuag gacauaugug cgacgcaacc augagcuacg aaugccccau gcuagacgaa      240

ggcgucgaac ccgacgacgu agacugcugg ugcaacacaa cuucaacaug ggucgucuac      300

ggaacaugcc accacaaaaa gggcgaagca agacgcuccc guagagccgu gacccugcca      360

agccacucaa ccagaaaacu gcaaacaaga ucacaaacau ggcuagaaag ccgcgaguac      420

acgaaacacc ucauaaaagu agaaaacugg aucuuccgca accccggguu cgcacucgua      480

gccgucgcaa uagccuggcu gcucggcucc agcacauccc agaagguaau auaccuaguc      540

augauccugc ucaucgcgcc agcauacucc auacgaugca ucggcgucag caacagagau      600

uucguagagg gaaugagcgg cggcaccugg guagaugucg uccuagaaca cggaggcugc      660

gucaccguaa uggcacagga caagcccaca guagacauag agcuagucac gacaacaguc      720

agcaacaugg cagaaguaag aagcuacugc uaugaagcau cuaucagcga cauggcgagc      780

gacagccgau gccccacgca aggcgaggcc uaccucgaca agcaguccga cacucaauac      840

guaugcaaac gaacccucgu agaccggggu uggggaaacg gaugcgggcu auucggaaaa      900

ggcucccuag uaaccugcgc caaauuuacc ugcuccaaga aaaugaccgg aaaaucgauc      960

caaccagaaa accuggaaua uagaauaaug cucagcgucc acggcagcca acauucuggc     1020

augauaggcu acgaaaccga cgaggacagg gcaaaagucg agguaacgcc aaacagccca     1080

agggcagaag cgacccucgg aggauucggc agccucggac ucgauugcga accccgcaca     1140

ggccucgacu ucucagaccu cuacuaccuc acaaugaaca acaaacacug gcucguacac     1200

aaggaauggu uccacgacau accacugccc uggcaugccg gcgcugauac aggaacacca     1260

cacuggaaca acaaagaagc ccucguggag uucaaagacg cccacgcaaa acgccaaacc     1320

guaguagucc uaggaagcca agaaggcgcg guacacacag cccucgccgg agcccuagaa     1380

gcagaaaugg acggagccaa agguagacua uucucuggac accucaaaug ccgauuaaaa     1440

auggacaaac uccgacuaaa ggguguauca uauagccuau gcacagcagc cuucacauuc     1500

acaaaggugc cagcagaaac gcuucacggg accgucaccg ucgaaguaca guacgccggc     1560

acagacggac cgugcaaaau accaguccag auggcaguag acaugcaaac acucacaccg     1620

gucggcaggc uaauaacagc caacccagua auaacagaaa gcacagagaa cagcaagaug     1680

augcuagagu uagacccgcc cuucggggac agcuacauag uaauaggcgu cggagacaag     1740

aaaauaacac accacuggca ccgaucaggc agcacgaucg gaaaagccuu cgaagcaacc     1800

guaaggggcg ccaaaagaau ggcaguacuu ggagacacag caugggacuu cggaagcguc     1860

ggcggaguau ucaauaguuu aggcaaaggc auacaccaga uauucggagc cgccuucaaa     1920

ucacuauucg gagggauguc augguucagc caaauccuca uaggcacccu acucguaugg     1980

cuaggccuca acaccaaaaa uggcuccaua agucugacau gccucgccuu aggcggcgua     2040

augauauucc uaucaaccgc cguaagcgcc                                      2070


<210>  9972
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9972
augggaaaac gcucagcggg aucaauaaug uggcucgccu cccuagcagu cgucaucgcc       60

ugcgccggag ccgucacucg ccgcggcucc gcauacuaca uguaccucga ccgaaacgac      120

gccggagaag ccauaucauu ccccacaacc cucggaauga acaaaugcua cauccaaauc      180

auggaccuag gccauaugug cgacgcaacg augucauacg aaugccccau gcucgacgaa      240

ggaguagaac ccgacgacgu agacugcugg ugcaacacaa cgagcaccug ggucgucuac      300

gguaccugcc accacaagaa aggcgaagcc cgcagaucca gacgagcagu aacucuccca      360

ucccacagca cccgcaaacu acagacaagg ucgcaaaccu ggcucgaaag cagagaauac      420

acaaaacacc ucauccgcgu agagaacugg aucuuccgaa aucccgguuu cgcccuagca      480

gccgcagcaa uagcauggcu ccucggcuca agcaccaguc aaaaaguaau auaccuaguc      540

augauacucc uaauagcacc cgcauacagc auaagaugca ucggcguauc gaaccgcgac      600

uucgucgagg gaauguccgg aggaacgugg guugacguag uacuagagca cggcgggugc      660

guaacuguaa uggcccaaga caaacccaca guugacauag agcucgucac cacaacgguc      720

ucaaacaugg ccgaaguccg gaguuacugc uaugaagcga gcaucagcga cauggcaucc      780

gacucccgau gccccacaca aggcgaagcc uaccuagaca aacagucaga cacacaauac      840

gucugcaaac ggacacucgu cgacagagga uggggcaacg gcugcggccu cuucggcaaa      900

ggcagccuug ucaccugugc gaaauuugcc ugcagcaaga aaaugaccgg caaauccaua      960

caacccgaaa accuggaaua ccgaaucaug cugucagucc acggauccca acacagcgga     1020

augaucguua acgacacagg ucacgaaacc gacgaaaaua gagccaaagu agaaauaaca     1080

ccgaacagcc cccgagccga agccacacua ggagguuuug gcucccucgg ccucgacugc     1140

gagccacgca ccggacucga uuucucagac cucuacuacu uaacgaugaa caacaaacac     1200

uggcucgucc acaaagaaug guuccacgac aucccccucc cauggcacgc aggagcagac     1260

acaggcacac cacacuggaa caacaaggaa gcccuagucg aguucaaaga cgcccaugcc     1320

aaacgccaaa ccguagucgu gcucgguuca caagaaggcg ccguccacac ggcacucgcc     1380

ggagcacucg aagccgaaau ggacggcgca aagggacgac uauccucagg ccaccucaaa     1440

ugcagacuca aaauggacaa acuaaggcua aaaggcguaa gcuacucccu auguacagca     1500

gcauucacau ucaccaaaau cccagcagaa acacuucacg gcacagucac aguagaaguc     1560

caauacgccg ggacagacgg gccaugcaaa gugccggcac agauggccgu agacaugcaa     1620

acccuaaccc ccguaggccg ucucaucacc gccaacccag uaaucaccga aagcaccgaa     1680

aacuccaaaa ugaugcuaga acucgaccca cccuucgggg acaguuacau cguaaucgga     1740

guaggggaaa agaaaaucac ccaccacugg caccgauccg gcuccaccau cgggaaggcu     1800

uucgaagcaa cgguacgcgg cgcaaaacgg auggcgguac ucggagacac agccugggac     1860

uucggcagcg ucggcggagc ccugaacuca cuagggaaag gaauacacca aaucuucggc     1920

gccgccuuca aaucgcuauu cgggggcaug agcugguucu cacaaaucuu aaucggaacc     1980

cuacucaugu gguugggccu gaacaccaag aacggaucca ucucacugau gugucuagcu     2040

cucggcgggg uacucaucuu ccugagcacc gcagucagcg cc                        2082


<210>  9973
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9973
auggccaucu caggcguacc aguccuaggg uucuucauca ucgccguccu caugagcgcc       60

caagaauccu gggccguaac ccgccgagga uccgccuacu acauguaccu agacagaaac      120

gacgcaggag aagccaucuc cuucccgaca acacucggaa ugaacaaaug cuacauacaa      180

aucauggacc ucggccacau gugcgacgca acaaugagcu acgaaugucc caugcuagac      240

gaaggagucg aacccgacga cgucgacugc uggugcaaca cuaccagcac cugggugguc      300

uacggaaccu gccaccacaa aaagggcgaa gcacgacgaa guagacgcgc cgucacccuc      360

cccagccacu caacacguaa acuacaaacc cgaagccaga ccuggcucga aagccgagaa      420

uacaccaaac accucaucag aguagaaaau uggaucuuca gaaaccccgg guucgcacug      480

gcggcagccg ccaucgccug gcuacucgga ucuagcacgu cccaaaaggu cauauaccug      540

gucaugaucc uacuuaucgc cccagcauac uccauccgcu guauaggcgu auccaaccgu      600

gacuuugucg aaggcauguc cggcggaacc ugggucgaca uaguccucga acacggcgga      660

ugugucaccg uaauggcaca ggacaaacca acagucgaca ucgagcuagu aaccaccacc      720

gucuccaaua uggcagaagu aagauccuac ugcuacgaag ccucaaucag cgacauggcc      780

ucagacagcc gaugccccac acaaggcgaa gcauaccuag auaagcaguc agacacccag      840

uacgucugca agcgcacccu agucgaccgc ggauggggaa acggcugcgg gcuauucgga      900

aaaggaagcc uaguaaccug cgccaaguuc gcuuguucaa agaaaaugac cggaaaaucc      960

auacaaccag aaaaccucga auaccgcauc augcuauccg uacacggcuc acaacacagc     1020

ggcaugauag uaaacgacac cggacacgaa accgacgaaa accgggccaa agucgaaaua     1080

acaccgaaua gcccccgcgc cgaagcaacc cucggcggau ucggaucccu cggguuagac     1140

ugcgagcccc gcacuggccu cgacuucucc gaccuauacu accucaccau gaacaacaaa     1200

cacuggcugg uccacaaaga gugguuccac gacauccccc ugccauggca cgcaggagcc     1260

gacaccggca caccccacug gaacaauaaa gaagcucuag uagaguucaa ggacgcacau     1320

gcaaaacgac aaaccgucgu cguccuaggc ucccaagaag gagcaguaca caccgcccuc     1380

gcaggagccc ucgaagccga aauggacgga gccaaagggc gacucuccuc aggacaccua     1440

aaaugucgcc uaaaaaugga caagcuaagg cucaaaggag uaagcuacuc ccucugcacc     1500

gcggcauuca ccuucacaaa aauccccgcu gaaacacucc acggcaccgu cacaguggaa     1560

guacaauacg ccggaacaga cgggccgugc aaaguaccag cccaaauggc ggucgacaug     1620

cagacccuca ccccagucgg gcgccucauc acggccaacc caguaauuac ggaaucaacc     1680

gagaacucca aaaugaugcu cgaacuggau cccccauucg gcgacagcua caucgucauc     1740

ggcguuggcg aaaagaaaau aacacaccac uggcacagau ccggcagcac acucggcaaa     1800

gcguucucua caacacucaa aggugcacaa cgacuagcug cacucggcga cacagcaugg     1860

gacuucggau ccaucggcgg agucuucaac uccaucggga gagcagucca ccagguuuuc     1920

gggggcgccu uccgaacacu auucggaggg augagcugga ucacgcaagg acuaauggga     1980

gcccuccuac uauggauggg cguaaaugca agagaccgga gcauagcacu cgccuuccua     2040

gcaaccggag gcguccucgu cuuccuagcc acaaacgucc acgcg                     2085


<210>  9974
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9974
auggccauca gcggcguccc cguccuaggc uuuuucauca uugccguacu caugucagcc       60

caagaaagcu gggccguaac ccgacgcggc agcgccuacu acauguaccu agaccgaaac      120

gaugcagggg aagcaaucuc cuuccccacc acgcuaggaa ugaacaaaug cuacauucaa      180

auaauggacc ucggccauac cugcgacgcc accaugucau acgagugccc cauguuagac      240

gaaggaguag aacccgacga uguggauugc uggugcaaca caacaagcac cugggucgug      300

uacgggaccu gccaccacaa gaaaggagaa gcgcgcagau cacgcagggc agucacgcua      360

cccagccacu caacacggaa acugcaaacc cgcucccaaa cauggcuaga gucacgggaa      420

uacaccaaac accugauccg agucgaaaac uggaucuuua gaaacccagg auucgcccuc      480

gccgcagcug cuaucgccug gcuccucggg ucaucaacau cccagaaagu uaucuaucuc      540

guaaugaucc uccuaaucgc ccccgccuac uccauccgcu gcauaggagu gaguaacaga      600

gacuucgugg aaggcauguc cggcgggaca uggguagacg ucguacucga acacggggga      660

ugcguaacag ugauggcuca agauaaacca acaguagaca ucgaacucgu uacgacaacc      720

gucagcaaca uggcagaagu ccgaagcuac ugcuacgagg caagcauaag cgacauggcc      780

ucagacagcc gaugcccuac ccaaggcgaa gcauaccuag acaaacaaag cgacacccaa      840

uacgucugca aaagaacgcu cguggaccgu ggcuggggaa acggcugcgg ccuauucgga      900

aaaggaaguc ugguaaccug cgccaaauuc gcgugcucca agaaaaugac cggcaaaucc      960

auccaaccug aaaaccucga auaccggauc augcuauccg uccacggcag ccaacacuca     1020

ggcaugaucg ucaacgacac aggacacgaa accgacgaaa aucgagccaa aguagaaaua     1080

acacccaaca gcccaagagc agaagcaaca cuaggcggau ucgggagccu cggacucgac     1140

ugcgagccac gcacagggcu agacuucuca gaccucuacu accuaaccau gaacaacaaa     1200

cacuggcucg uccacaaaga augguuccac gacauaccac uccccuggca cgcaggggcc     1260

gacaccggca cgccacacug gaacaacaaa gaggcacuug uagaguucaa agacgcccac     1320

gcaaagcgac aaaccgucgu aguucucgga ucccaagaag gcgccgucca cacggcccuc     1380

gccggagcac uagaagcaga aauggacggc gccaaaggaa ggcuaaguuc cggccaccuc     1440

aaauguagac ucaaaaugga caaacuucga cuaaaaggag ucagcuacag cuuaugcacc     1500

gccgcauuca cauucacaaa aaucccagca gaaacacuac acggaaccgu cacagucgaa     1560

guacaauacg cgggcacaga cggcccaugu aaggugcccg cccaaauggc aguggacaug     1620

caaacccuaa cccccguagg gcgacucauc acggcaaacc cagucaucac cgaaagcaca     1680

gaaaacagca aaaugaugcu cgaacucgac ccaccuuucg gggacuccua cauagucauc     1740

ggcgucggcg aaaagaaaau aacccaccac uggcacagau caggcaguac ccuuggaaaa     1800

gccuucucaa ccacuuuaaa aggugcacaa agacuagcug cacucggcga caccgcaugg     1860

gacuucggcu cgauaggcgg gguauucaac agcauaggac gagcggucca ccaagucuuc     1920

ggcggcgcau uccggacacu guucggagga auguccugga uaacccaggg acucaugggg     1980

gcccuacucc ucuggauggg aguaaacgcg agagaccgcu ccaucgcccu agcauuccua     2040

gccacaggag gcguccuagu uuuccucgca acaaacguac augcc                     2085


<210>  9975
<211>  2073
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9975
auggccaucu cagggguacc aguacuaggc uucuucauca uagccguccu caugucagcc       60

caagaaucau gggcgauaac acgccgaggc agcgcuuacu auauguaucu cgaucgaagc      120

gacgccggaa aagccaucag uuucgcuacc acucuaggag uuaacaaaug ccacguacaa      180

aucauggauc ugggccacau gugcgacgcc accaugagcu acgagugccc aaugcucgac      240

gaaggaguag aaccggacga cgucgacugc uggugcaaca ccaccaguac auggguuguc      300

uacggcacuu gccaccacaa gaaaggagaa gcccgacgau cccgccgcgc ggucacucuc      360

cccucccacu ccacacgaaa acuccaaacc cgcucacaaa cguggcuaga gucgcgagaa      420

uacaccaaac accucauaaa aguagaaaac uggaucuucc gcaacccagg cuucgcacuc      480

guagcgguug cgaucgcgug gcuccuaggc uccuccacuu cccaaaaagu caucuaccua      540

guuaugaucc uccuaaucgc cccagccuac agcauccggu gcaucggagu cucaaacaga      600

gacuucgucg aagguaugag cggcggcaca uggguagacg ugguccucga acacggcgga      660

ugcgucacag uuauggcgca agacaaaccc accguagaca ucgaacucgu cacgaccacc      720

gugucaaaca uggccgaagu ccgaagcuac ugcuacgaag caucaauauc agacauggcc      780

uccgacuccc guugcccaac gcaaggagaa gccuaccucg acaaacaaag cgacacccaa      840

uacguuugca aacgcacccu agucgaccgc ggcuggggca acggaugcgg ccucuucgga      900

aaaggcuccc ucgugacaug cgcaaaauuc acaugcagca agaaaaugac aggaaaauca      960

auccaacccg aaaaccucga auacagaauc auguugucag uacacggcag ccaacacuca     1020

ggaaugauag gauacgaaac ggacgaagac cgcgcaaaag ucgaagucac cccaaacagc     1080

cccagggccg aggcgacccu uggaggauuc ggcagccucg gacucgacug cgagccccgc     1140

acaggccucg auuucagcga ucuauacuac cuuaccauga auaacaaaca cugguuaguc     1200

cacaaagaau gguuccacga cauaccccuc cccuggcacg cgggagcgga caccgguacc     1260

ccacacugga acaacaaaga agcacucguc gaguucaaag acgcccacgc caaaaggcaa     1320

acaguaguag uccucggaag ucaagaaggc gccguccaca ccgcacuggc cggcgcccuc     1380

gaagccgaaa uggauggagc caaaggccga cucuucucag gacaccucaa augccgacuc     1440

aaaauggaca aacucagacu aaaaggagua uccuacagcc uuugcacagc cgccuucacg     1500

uucacaaaag uccccgccga aacacuucac ggaacgguca cagucgaagu acaauacgcc     1560

ggcaccgacg gccccugcaa aauccccguu caaauggcgg uggacaugca aacucucaca     1620

ccuguaggac gacuaauuac cgccaacccc gucaucacug aauccacaga aaacagcaaa     1680

augaugcuag aacucgaucc cccauucggc gacagcuaca uaguaaucgg ugucggcgac     1740

aagaaaauca cacaccacug gcaccgcagc ggcuccacac uuggaaaagc auuuucaacc     1800

acccucaaag gcgcacaaag acuagccgcg cucggagaca cagcauggga cuucggcucg     1860

aucggcggag ucuucaacuc cauaggacga gccguccacc agguauucgg cggagccuuc     1920

cgcacacugu ucggaggcau gagcuggauc acucaaggac ucaugggagc gcuccuacuc     1980

uggaugggcg ucaacgcccg cgaccgaagc auagcuuuag ccuuccucgc cacaggaggc     2040

guccucgucu uccucgcgac caacguacac gcc                                  2073


<210>  9976
<211>  2085
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9976
auggcgaucu ccggcguacc aguccuagga uucuucauca ucgcaguccu caugucugca       60

caagaaucau gggcaguaac aagacgcggg uccgcauacu acauguaccu cgaucgaaac      120

gaugccggcg aagccauauc cuuccccaca acacuaggca ugaacaaaug cuacauccaa      180

aucauggacc uaggacacau gugcgacgca acaauguccu acgaaugccc aaugcuggac      240

gagggggugg aaccagacga cguagauugu uggugcaaca caaccuccac cuggguggua      300

uacggaacgu gccaccacaa aaagggagaa gcccgacgca gccgacgagc cgugacccua      360

cccucccaca gcacacgaaa acuacaaaca cgcagccaaa cauggcuaga aagcagagaa      420

uacaccaaac accuaauacg cguagaaaac uggaucuucc gcaacccagg cuucgcacug      480

gccgcagccg ccaucgcaug gcuccucggc agcucaaccu cccaaaaggu aaucuaccuu      540

guuaugaucc uccuaaucgc accggcguac uccauaagau gcaucggcgu cucaaauagg      600

gacuucguag aaggcaugag cgggggaacg uggguagacg uaguccucga acacggaggc      660

ugcguaaccg ugauggcaca agacaaacca acgguagaca ucgagcuagu aacaacaacc      720

gucuccaaca uggcugaagu cagauccuac uguuacgaag ccagcauaag ugacauggcc      780

ucagacucac gcugccccac acaaggcgaa gcauaccuag acaaacaauc cgacacccag      840

uacgucugca agcgaacccu cgucgacaga ggcuggggca acggaugugg ccuauuugga      900

aagggaagcc ucguaaccug cgcuaaauuc gccugcucaa agaaaaugac aggcaaaagc      960

auccaacccg aaaaccugga auaccgcauc augcuaagcg uccacggcag ccaacacuca     1020

gggaugauag uaaaugauac cggacacgaa acagacgaaa accgagcaaa aguggaaaua     1080

acaccaaacu cgccacgggc cgaagccacg cuagggggcu ucggcucacu agggcucgau     1140

ugcgagccaa gaaccggacu agauuucagc gaccuauacu aucuaacgau gaacaacaaa     1200

cauuggcuag uacacaaaga augguuccac gacauccccc uaccauggca cgccggcgcc     1260

gacaccggca caccacacug gaacaacaaa gaagcacucg uggaguucaa agaugcccac     1320

gccaaaagac aaacagucgu gguacuaggc agucaagaag gcgccgugca cacagcucuu     1380

gcgggagccc uagaagccga aauggacggc gccaaaggaa gacuaagcuc cgggcaccuc     1440

aaaugccgac uaaaaaugga caaacucaga cugaaaggcg ucagcuacuc acucugcaca     1500

gcagccuuca cauucaccaa aauucccgca gaaacacuac acggcacagu aaccgucgaa     1560

guccaauacg caggcacaga cggccccugc aaaguccccg cacaaauggc cguagacaug     1620

caaacacuaa cacccguagg ucgacuaaua accgcgaacc cagucaucac agaauccacu     1680

gaaaacagca aaaugaugcu agaacucgac ccgccauucg gagacucaua caucguaauc     1740

ggagucggag agaagaaaau aacacaccau uggcaccgcu cgggcuccac acuaggcaaa     1800

gccuucagca caacccucaa aggagcacaa agacuggccg cacucggcga caccgcgugg     1860

gacuucggca gcaucggagg cguguucaac ucaauaggac gugccguaca ccaagucuuc     1920

ggcggagccu uccgaacacu auucggcggg augucaugga uaacccaagg ccuaaugggc     1980

gcccuacucc uauggauggg aguaaacgca cgggaccgcu cgauagcacu agcuuuccuc     2040

gcaacgggcg gcguccuagu guuucuagca acaaacguuc acgcc                     2085


<210>  9977
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9977
auggacugga ccuggauacu auuccucgua gccgccgcca cccgggucca uucagucacc       60

cgccgaggcu cugccuacua cauguaucug gacagaaacg acgccggcga agcaauuagc      120

uucccaacaa cccucggaau gaacaaaugc uacauacaaa uaauggaccu cggccacaug      180

ugcgacgcaa ccaugucaua cgagugccca augcuggacg agggagucga acccgacgac      240

guagacugcu ggugcaacac caccagcacc ugggucguau acggcacaug ucaccacaag      300

aaaggggaag cccgacguag ccgcagagca guaacccuac caagucauuc aacacgcaaa      360

cuccaaacaa ggagccaaac cuggcuagaa ucacgggagu acacaaaaca ccucauacga      420

guagaaaacu ggaucuucag aaacccgggg uucgcccucg cagccgccgc cauagcaugg      480

cuucucgggu caagcaccuc ccaaaaagua aucuaccucg ucaugauacu acuaaucgca      540

ccagcauaca gcauacgaug caucggcgua ucaaacagag acuucguaga aggcaugagu      600

ggagggaccu ggguugacau aguacuggaa cacggcggau gcgucacagu cauggcacag      660

gacaaaccaa ccgucgacau agagcuuguu acaaccacgg ucagcaacau ggccgaaguc      720

cgaagcuacu gcuacgaggc aagcauauca gacauggccu cagacagccg augcccaacg      780

cagggagaag cauaccucga caaacaaagc gacacgcaau acgucugcaa aaggacgcuu      840

guagaccgcg gcuggggaaa cggaugcggc cucuuuggaa aaggaucucu aguaaccugc      900

gcaaaguucg cgugcucaaa gaaaaugacu ggaaaaucaa uccaacccga gaaccucgaa      960

uaccgcauca ugcuauccgu ccacggguca caacacagcg gaaugaucgu aaacgacaca     1020

ggacacgaaa cagacgaaaa cagggccaaa guagagauca ccccgaacag cccaagagcc     1080

gaagcaacac uaggcggcuu cggcagucuu ggacucgacu gcgaaccgcg gacuggacug     1140

gacuuuagcg accuguacua ucucaccaug aacaacaaac acuggcuagu acacaaagaa     1200

ugguuccacg acaucccgcu cccauggcac gcaggcgccg auaccggaac cccucacugg     1260

aacaacaaag aagcccucgu agaguucaaa gacgcccacg ccaaacgaca aacaguaguc     1320

gugcucggaa gccaagaagg agcgguacac acggcacucg cgggagcccu ggaagcggaa     1380

auggacggcg caaaaggacg acuaucaagc ggucaccuca aaugccgacu aaaaauggac     1440

aagcuacgcc ucaaaggcgu cagcuauagc cuaugcacag ccgcauucac auucacaaaa     1500

auaccggccg aaacacuaca cggcaccguc accgucgagg uacaauacgc agguaccgac     1560

ggacccugca aagucccagc ccagauggca gucgacaugc aaacccucac cccugucggg     1620

aggcuaauca ccgcaaaccc cguaauuaca gaaucaacag agaacagcaa aaugaugcug     1680

gaacuggacc cacccuucgg cgacuccuac aucgucaucg gaguagguga aaagaaaauc     1740

acccaccacu ggcaccgaag cggcucaacc cuaggcaaag ccuucagcac aacccuaaaa     1800

ggagcccaac gccucgccgc ccucggcgau acggcguggg acuucggcuc cauaggaggc     1860

guauucaauu caaucggccg cgccguacac caagucuucg gcggagcauu ccggacacuc     1920

uucggaggaa ugagcuggau aacgcaagga cucaugggcg cauuacucuu auggaugggg     1980

guaaacgccc gagaccguag caucgccuua gccuuccucg ccacaggcgg agugcuagua     2040

uuccucgcaa caaacgucca cgcc                                            2064


<210>  9978
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9978
auggacugga ccuggauacu cuuccuggua gccgcagcua cccgcguaca cucagucaca       60

cgccgaggau cggccuacua cauguacuua gaccgcaacg acgcuggcga agcaauauca      120

uuccccacaa ccuuaggcau gaacaaaugc uacauacaaa ucauggaccu gggccacaca      180

ugugacgcga ccauguccua cgagugccca augcuggacg aaggaguaga gccggacgac      240

guagacugcu ggugcaacac caccagcaca uggguagucu acggaaccug ccaccacaag      300

aaaggcgagg cccgccgaag ccgaagggca gucacccucc caagccacuc cacccgaaaa      360

cuacagaccc gcucccaaac cuggcuagaa ucucgugaau acacaaaaca ccucaucagg      420

guagaaaacu ggaucuuccg caaccccggc uucgcacucg cagcagccgc aaucgcuugg      480

cuacugggcu ccuccaccuc acaaaaagua auauaccugg ucaugauccu acugaucgcc      540

ccagccuaca gcauccgcug caucggcgua agcaaccggg acuucguaga aggcaugucu      600

ggcgggaccu ggguagacgu cgugcuagag cacggcggcu gcgucacagu aauggcacaa      660

gacaagccca cagucgacau cgaacucguc accaccaccg uaagcaacau ggcagagguc      720

cgcuccuauu gcuacgaggc cagcauauca gacauggcaa gcgacagccg gugcccaacc      780

caaggcgaag cauaccucga uaaacaaucc gacacgcaau acguaugcaa acgaacacuc      840

gucgacagag gcuggggaaa cggaugcggc cuauucggaa aaggaucccu agucaccugu      900

gccaaauucg ccugcaguaa gaaaaugaca ggcaaaagca uacaaccaga aaaccuagaa      960

uaccgaauca ugcucuccgu acacggaagc caacacucag gaaugaucgu caacgacacu     1020

gggcacgaaa ccgaugaaaa ccgugcaaaa guagaaauaa cgcccaacuc accacgcgca     1080

gaagccaccc ugggaggcuu cggcagccua ggacucgacu gcgaaccaag aacuggccuc     1140

gacuucucag accucuacua ccucaccaug aacaacaaac acuggcucgu acacaaagag     1200

ugguuccacg acaucccccu accauggcac gcuggggcgg acacaggcac accccacugg     1260

aacaauaaag aagcccuagu agaguucaaa gacgcacacg caaaacgaca aaccguaguc     1320

guacuuggca gccaggaagg ugcgguccau accgcacuag ccggcgcccu cgaagccgaa     1380

auggacggcg cuaaaggucg ccucucaagc ggccaucuaa agugccgacu aaaaauggac     1440

aaacuccguc ucaaaggagu cuccuacagc cugugcaccg cagcauucac cuucacaaaa     1500

aucccagcgg aaacccucca uggaaccgug accgucgaag uccaauacgc aggaacagau     1560

ggacccugca agguccccgc ccaaauggca guagacaugc aaacacugac ccccgucggc     1620

cgacucauaa cggccaaccc agucauaacu gaauccacag aaaacucaaa aaugaugcug     1680

gagcucgacc cgcccuucgg cgauagcuac auagucaucg gcgugggcga aaagaaaaua     1740

acucaccacu ggcaccgcag cggcaguaca cuaggcaaag ccuucaguac cacccugaaa     1800

ggagcccaac gacucgcagc ccucggagac acagcauggg acuucgguag caucggcggc     1860

gucuucaacu ccaucgggag agccgugcac caaguguucg gaggcgccuu ccguacccuc     1920

uucggaggaa ugucauggau aacucaaggg cucaugggcg cccuccuacu cuggauggga     1980

guaaacgcca gagacagaag caucgcccua gccuuccuag ccaccggcgg aguacucgua     2040

uuccuggcaa ccaacgucca cgcu                                            2064


<210>  9979
<211>  2052
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9979
auggauugga ccuggauccu guuccucgua gccgccgcca cacgcguaca uucaauaaca       60

cgccgaggau cggcauacua cauguacuug gaccguagcg acgcaggcaa agcgaucucc      120

uucgccacaa cccugggcgu caauaaaugc cacguacaaa ucauggaccu uggacacaug      180

ugcgacgcca caaugucaua cgaaugcccc augcuggacg agggcguaga accagacgac      240

gucgacugcu ggugcaauac cacaagcaca ugggucguuu acggcacaug ccaccacaag      300

aaaggggaag cgcgacgguc ccgacgagcg gugacacuac caucccacag cacacgcaaa      360

cuacaaacaa ggagccaaac cuggcucgaa agccgagagu acacaaaaca ccuaauuaaa      420

guagaaaacu ggaucuuccg caauccagga uucgcgcuag uagcagucgc cauagccugg      480

cuacuaggaa gcagcaccuc ccagaaaguc aucuaucuag ucaugauccu cuuaauagca      540

cccgcguaca guauacgaug cauaggcgua agcaacaggg auuucguaga aggaaugucg      600

ggagggaccu ggguagacgu cgugcucgag cacggcgggu gcguaaccgu aauggcccaa      660

gacaaaccca cagucgacau cgaacucguu acaaccacag uuagcaauau ggccgaagua      720

aggucauacu gcuacgaagc aucgaucagu gacauggcau cagacucacg gugccccacg      780

caaggcgaag cauaccuaga caaacaaagc gacacccaau acgucugcaa acgaacacuc      840

gucgaccgag gcuggggaaa cgggugcgga cucuucggca aaggcucgcu gguaaccugc      900

gcaaaauuua cauguucaaa gaaaaugaca ggcaaaagca uucaacccga aaaccucgag      960

uaccgaauca ugcuaucagu ccacggaagc caacacagcg gaaugaucgg guaugaaacc     1020

gacgaggaca gagccaaagu agaaguaacg ccaaacagcc cccgggccga agccacacug     1080

ggcggauucg gaagccucgg ccuggacugc gaacccagaa caggccugga cuucagcgac     1140

cuauacuacc uaaccaugaa caacaaacac uggcucguac acaaagagug guuccacgac     1200

aucccccucc cauggcacgc aggagccgac accggcacac cccacuggaa caacaaagaa     1260

gcccuggucg aguucaaaga cgcccacgcg aaacgacaga ccguagucgu gcuaggaagu     1320

caagaggggg caguacacac agcccucgcc ggagcacucg aggccgagau ggacggcgca     1380

aaaggacgcc ucuucucggg acaccuaaaa ugccgccuca aaauggauaa auuaagacua     1440

aaaggcgucu cauacucguu augcaccgcc gcauucaccu ucaccaaagu ccccgccgaa     1500

acccuacaug gaacaguaac cguggaagua caguacgcgg ggaccgacgg acccugcaaa     1560

aucccagucc agauggcggu cgacaugcaa acccugacgc ccgucggacg ccuaauaacg     1620

gcuaacccgg ugauaaccga auccacagaa aacucaaaaa ugaugcucga acuagaccca     1680

ccauucggag acagcuacau cguaauagga gucggcgaca agaaaaucac ccaccacugg     1740

caccgaagug gauccacacu aggcaaagca uucucaacca cacucaaagg ggcccagcgg     1800

cucgccgccc uaggagacac agcaugggac uucggaucaa ucggaggugu auucaacucg     1860

auaggcagag ccguccacca aguauucgga ggggcauucc gaacacuauu cgggggaaug     1920

agcuggauca cacaaggacu caugggagcc cugcugcuuu ggaugggcgu aaacgccaga     1980

gaccgaucaa uagcccuagc auuccuugca acaggaggcg uccucgucuu ccuagcaaca     2040

aacguacacg ca                                                         2052


<210>  9980
<211>  2064
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9980
auggacugga cauggauccu cuuucucgua gcggccgcaa cccgagucca cagcgugacc       60

cgccgcggcu ccgccuacua cauguaccuc gacagaaacg acgccggcga ggcaaucucc      120

uucccgacca cccucggaau gaauaaaugu uacauacaaa ucauggaccu cgggcacaug      180

ugcgacgcaa ccaugagcua cgaaugcccc augcuggacg aaggaguaga accagacgac      240

gucgauugcu ggugcaacac cacaagcacc uggguaguau auggcacaug ucaccacaaa      300

aagggggaag cccgccgcuc acgccgcgcc guaacacucc caucacacag cacccgcaaa      360

cuccaaacca gaucccaaac cuggcucgaa ucaagagagu acacaaagca ccuaauacgc      420

guagaaaacu ggauuuucag aaacccaggc uucgcucuug cagccgccgc gaucgccugg      480

cuccucggcu ccaguacaag ccagaagguc aucuaccucg uaaugauccu ccuaaucgca      540

ccagccuacu ccauacgcug cauaggugua ucaaaccgag acuucguaga aggaaugagc      600

ggcggaacau gggucgacgu aguacuagaa cacggcggcu gcgucacagu cauggcacag      660

gauaaaccaa cggucgacau cgaacucgua accacaacag uaagcaacau ggcagagguc      720

cgaagcuacu gcuacgaggc cuccauaucc gacauggcau ccgacuccag gugccccacc      780

caaggagaag ccuauuuaga caaacaaucc gacacccaau acgucugcaa aagaacacug      840

guggaucgag gaugggggaa cggcugcgga uuauucggaa aaggaucacu cguaaccugc      900

gcaaaauucg caugcagcaa gaaaaugaca ggcaaaagca uccagccgga aaaccucgaa      960

uaccgcauca ugcuaucagu acacggaucc caacacuccg gcaugaucgu gaacgacaca     1020

ggacacgaaa cggacgaaaa ccgggccaag gucgaaauaa caccuaacuc accccgagcc     1080

gaagcgacuc ucggaggguu cgggagccuc ggccucgacu gcgagccacg caccggccua     1140

gacuuuucag accucuauua ccucaccaug aacaacaaac acuggcuagu ccacaaagaa     1200

ugguuccacg acaucccacu accuuggcac gccggcgccg acacaggcac accccacugg     1260

aauaacaaag aagcacuggu cgaauuuaaa gacgcacacg ccaaacgaca aacagucguc     1320

guacuaggau cacaagaagg agcaguucac accgcccuag ccggagcacu ugaagcagaa     1380

auggacggag caaaaggacg ccuuagcuca ggacaccuaa agugcaggcu gaagauggac     1440

aaacugagac ucaaaggagu cuccuacuca cugugcaccg cagccuucac auucacaaag     1500

auccccgcag agacucucca cggaaccguc accgucgaag uacaauacgc aggaacagac     1560

ggcccuugca agguccccgc ccaaauggca gucgacaugc agacacucac cccagucgga     1620

agacucauca cagccaaccc cgucauaacc gaauccaccg aaaacucaaa aaugaugcuc     1680

gaacuggacc cacccuucgg ugacagcuac aucgucauag ggguaggaga aaagaaaauc     1740

acacaccacu ggcaccgcuc aggauccacc cucggaaaag ccuucucaac cacacucaaa     1800

ggcgcacaaa gacucgcggc acucggagac accgcguggg acuucggauc caucggcggc     1860

guauucaaca gcauaggacg cgcaguccac caaguauucg gaggagccuu ucgaacacuc     1920

uucggcggaa ugucauggau cacccaaggc cuaaugggcg cccuacuccu cuggauggga     1980

gugaacgccc gcgaccggag caucgcacua gcuuucuugg caacaggcgg cguccuagua     2040

uuccuggcca ccaacguaca cgcc                                            2064


<210>  9981
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant CDS opt

<400>  9981
augggcaaaa ggucagcggg cagcaucaug uggcucgccu cccucgcugu agugaucgcc       60

ugcgcaggag cgguaacgcg ccgaggcuca gcauacuaca uguaucugga ccgaaacgau      120

gccggagaag caauauccuu cccaacaaca cuaggcauga acaaaugcua cauacaaaua      180

auggaccuag gccacaugug cgacgcaacg augucauacg aaugcccaau gcucgacgaa      240

ggcguugaac cagacgacgu cgacugcugg ugcaacacaa cgagcacgug ggucguauac      300

ggcacaugcc accacaagaa aggagaagca cgccggagcc gucgggcagu cacccugcca      360

ucccacucca caagaaaacu ccaaacacga agccagacau ggcucgagag cagagaauac      420

accaaacacc uaaucagagu cgaaaacugg auauuccgaa accccggguu cgcgcucgca      480

gccgccgcaa ucgccuggcu acuaggauca ucaaccagcc aaaaaguaau cuaucuagua      540

augauacuac uaauagcccc ggcguacucc auaagaugca uaggcgucag uaaccgagau      600

uucguagaag gaaugucagg agggacaugg gucgacaucg uacuagagca cggcggcugc      660

gugaccguaa uggcacagga caaaccaacg guagacaucg aacucgucac cacaaccgua      720

aguaacaugg cagaagugag aucauauugc uacgaagccu ccauaagcga cauggccagc      780

gacucuagau guccaacgca aggagaagcg uaccucgaca aacaguccga cacgcaauac      840

gucugcaaac gcacacucgu ggaccgcggc uggggaaacg gaugcggacu auucggaaaa      900

gggucccuag ucacaugcgc aaaauuugcg ugcucaaaga aaaugacagg caaauccauc      960

cagccagaaa accuagaaua cagaaucaug cucuccguac acggcuccca acacagcgga     1020

augaucguaa acgacacagg acaugaaacg gacgagaaca gagcaaaagu cgaaaucacg     1080

cccaauagcc cacgcgccga agcaacccuu ggaggcuucg gcagcuuggg ccuugacugc     1140

gaaccacgca cggggcuaga cuucucagac cuauacuacc uaacaaugaa caacaaacac     1200

uggcucgucc acaaagagug guuccacgac aucccccucc cguggcacgc cggcgcagau     1260

accggcacac cccacuggaa caacaaagaa gcccuagucg aguucaaaga cgcacacgcc     1320

aaaagacaaa cagucguggu acuaggaucc caagaaggag caguccacac agcgcuagca     1380

ggcgcccuag aggccgagau ggauggagcg aaaggacguc uauccucagg acaccuaaaa     1440

ugcaggcuaa aaauggacaa acuacgacua aaaggcgucu cuuacucacu augcacagcc     1500

gcauucacau uuaccaaaau cccagcggaa acacugcacg gcacaguaac cguagaagua     1560

caauacgcag gcaccgacgg acccuguaag gucccagccc aaauggcagu cgacaugcaa     1620

acacugaccc cggucggacg ccugauaaca gccaacccag uaaucacaga aucgacagaa     1680

aauuccaaaa ugaugcuaga acuagauccc ccauucggcg acagcuauau cgugaucgga     1740

guaggggaaa agaaaaucac ccaccacugg caccgauccg ggagcacgcu cggaaaagcg     1800

uucucaacca cacugaaagg ggcacagcgg cucgcagcac ucggcgacac cgcaugggac     1860

uucgggucaa ucggaggcgu cuucaacucc aucggacgag caguacacca agucuucgga     1920

ggcgcguuca gaacauuauu ugggggcaug agcuggauca cacagggccu caugggcgcc     1980

cuacuccucu ggaugggggu gaacgccaga gacagaucca uugcacuagc guuccuagca     2040

acgggcggcg uacucguauu ccuagccaca aacguccaug cc                        2082


<210>  9982
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant CDS opt

<400>  9982
auggggaaaa gaucagcagg cuccaucaug uggcucgccu cacucgcagu agucauagca       60

ugcgcaggcg cggucaccag acgaggcuca gcguacuaca uguaccuuga caggaacgac      120

gcaggagaag ccaucagcuu cccgaccaca cucggcauga acaaauguua cauccaaauc      180

auggaccuag gacauaccug cgacgccaca augucauacg aaugcccaau gcuagacgaa      240

ggcguagaac ccgacgacgu cgacugcugg ugcaacacca cgagcaccug ggucgucuac      300

ggcacaugcc accacaagaa aggagaagcc cgccgauccc gccgagcagu cacccucccc      360

agccacucua ccagaaaacu acaaaccaga agccagaccu ggcucgaauc aagggaauac      420

acaaaacacu uaaucagggu cgaaaacugg auauuccgua acccaggguu cgcccucgcg      480

gcagccgcca uagcauggcu acugggcucg agcacuagcc agaaaguaau cuaccucguc      540

augauauuac ugaucgcacc agccuacuca aucagaugca uaggcguaag caaccgcgac      600

uucguagaag ggaugucagg uggaacaugg gucgacgucg uacucgaaca cggcgguugc      660

gucacaguaa uggcucagga caagcccacg guagacauag aacuuguaac aacaaccgua      720

agcaacaugg ccgaaguacg cuccuacugc uacgaagccu caaucucaga cauggcaucc      780

gacuccaggu guccaacgca aggggaagcg uaccuagaca aacaauccga cacacaauac      840

gucugcaaaa gaacccuggu cgaccgaggc uggggcaacg gaugcgggcu auuuggcaaa      900

ggcucccuag uaaccugugc caaguucgca ugcuccaaga aaaugaccgg caaauccaua      960

caacccgaaa acuuagaaua cagaauaaug cuaucuguuc acggauccca acacagcggg     1020

augauaguca augacacagg acacgaaaca gacgagaaca gggccaaagu agaaauaacc     1080

cccaacagcc cacgcgcaga agcuacacug ggcggauucg gaucucuagg ccuagacugc     1140

gaaccacgca ccggccuaga cuucucagac cucuacuacu ugaccaugaa uaacaaacac     1200

ugguuagucc acaaagaaug guuccacgac auaccacucc ccuggcacgc cggcgcagac     1260

acaggcaccc cacacuggaa caacaaagaa gcccucgucg aguucaaaga cgcacacgcg     1320

aaacggcaga ccgucguagu ccucggaagc caagaaggag cgguccacac agcccuagca     1380

ggcgcacuag aagcagaaau ggacggcgcg aaagggcgac uguccucagg gcaccucaaa     1440

ugucggcuca aaauggacaa acuacgccuu aaaggcguau cguacucacu augcaccgcg     1500

gccuucacau ucaccaaaau ccccgccgaa acacuacaug gaaccguaac cgucgaagua     1560

caauacgcag gcaccgaugg gcccugcaaa gucccugcac aaauggcagu cgacaugcaa     1620

acacucaccc cagucggccg acuaaucaca gcuaaccccg ugauaacgga guccacagaa     1680

aacucgaaaa ugaugcucga acuagacccg cccuucggcg acagcuacau aguuaucgga     1740

gucggugaaa agaaaauaac ccaccacugg caccgaagcg gaagcacccu cggaaaagcc     1800

uucuccacca cccucaaagg cgcccaaaga cuagcagccu uaggcgacac cgcgugggac     1860

uucggaucua uaggcggagu auucaacucc auaggacgcg caguccacca aguauucggg     1920

ggagcauuca gaacacucuu cggcgguaug agcuggauca cacaaggccu caugggagca     1980

cuccuacucu ggaugggcgu caacgcaagg gacagaucca ucgcccucgc guuucuagcg     2040

accgggggag ugcucguauu ccucgccacg aacguccacg ca                        2082


<210>  9983
<211>  2070
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant CDS opt

<400>  9983
augggaaaac gcagcgccgg auccaucaug uggcuagcau cgcucgcagu aguaaucgcg       60

ugcgccggug caaucacacg acgcggcagc gcauacuaua uguaccucga ccgaagugau      120

gccgggaagg cgaucucauu ugcaaccacg cuaggcguaa auaaaugcca cguccagauc      180

auggaccucg gccacaugug cgacgccacc augagcuacg aaugcccgau gcuggacgaa      240

ggcguggaac ccgaugacgu cgacugcugg ugcaacacaa caagcaccug gguugucuac      300

ggcacgugcc accacaagaa aggcgaagca agaaggucac gacgagcagu aacgcuacca      360

agccacagca cacgcaaacu acaaaccaga ucacaaacau ggcucgagag ccgcgaauac      420

accaaacacc uuauaaaagu agaaaacugg auauucagaa acccgggauu cgcccuagua      480

gcaguagcaa uagcauggcu acugggcucg uccacgaguc aaaaaguaau auaccuagua      540

augauacuac ugauagcacc cgccuacuca auccggugca ucggaguauc caacagggac      600

uucguagaag ggaugucagg cgggacaugg guagacgucg uccuagagca cggcggaugc      660

gucacgguaa uggcccaaga caaacccaca guagauaucg agcuaguaac cacgaccgua      720

agcaacaugg ccgaagucag aucguacugc uacgaagcca gcaucagcga cauggccucc      780

gacagccgcu gcccaaccca aggggaagcc uaccuagaca aacaauccga cacacaauac      840

guaugcaaaa ggacccuagu agauagaggg uggggaaacg gauguggucu cuucggcaaa      900

ggcucccucg uaacaugcgc caaauucaca ugcagcaaaa agaugacagg caagucaaua      960

cagccagaaa accucgagua cagaaucaug cucucagucc acgguucaca acacagcgga     1020

augaucggcu acgaaacgga ugaagacaga gcaaaagugg aaguaacacc uaacagccca     1080

cgcgcagaag cgacacuagg aggauucggg agucuaggau uggacugcga accaagaaca     1140

ggccuagacu ucagcgaccu cuacuaccuc acaaugaaca acaaacacug gcuaguccac     1200

aaggaauggu uccacgacau accacuacca uggcacgcgg gcgccgacac cggaacaccg     1260

cacuggaaca acaaagaagc ccucgucgag uucaaagacg cgcacgcaaa acgacaaacg     1320

guaguagucc ugggcucaca ggaaggcgca guacacacag cgcuagccgg agcccuagag     1380

gcagaaaugg acggcgcaaa aggccgccug uucagcggac accuaaaaug cagacucaaa     1440

auggacaaac uacgccuaaa aggcguguca uacagccucu gcacggccgc cuuuacguuc     1500

acaaaagugc cagcagaaac acugcacggc acaguuacag uagaaguaca guaugccgga     1560

accgacggcc caugcaaaau cccugugcag auggccgugg acaugcaaac acugacaccg     1620

guaggacgac uaauaaccgc aaauccagug auaacggaau caacagaaaa cuccaaaaug     1680

augcuggaac ucgacccacc auucggcgac agcuacaucg ugauaggcgu aggagacaag     1740

aaaauaacac accacuggca ccggagugga agcacgcugg gaaaagcauu cagcacaacg     1800

cucaaaggcg cccagagacu cgcggcacuc ggcgacaccg caugggacuu cggcucaauc     1860

ggcggggucu uuaacagcau cggacgcgca gugcaccaag ucuucggagg cgcguucaga     1920

acccucuucg gaggcauguc cuggauaacc caaggccuca ugggcgcacu gcuacuaugg     1980

auggggguca acgcgagaga cagaagcaua gccuuagccu uccuggccac cggaggagua     2040

cugguauucc uagcaacaaa cgugcacgcg                                      2070


<210>  9984
<211>  2082
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant CDS opt

<400>  9984
augggaaaac ggagcgcagg cuccauaaug uggcuagcaa gccuagccgu cgugaucgcg       60

ugugcaggcg ccgucacccg gagaggcucg gcguacuaca uguaccucga ccgaaacgac      120

gcaggagagg caaucucauu ccccacaaca cucggaauga acaaauguua cauccaaaua      180

auggaccuag gccacaugug cgacgcaaca augagcuacg agugcccaau gcucgacgag      240

ggaguagaac cagacgacgu cgauugcugg ugcaacacaa ccagcaccug ggucgucuac      300

ggaaccugcc accacaagaa aggcgaagca cgccgaagca ggcgcgcagu cacucuaccg      360

agucauagca cgcgcaaacu ccaaacacgc agccaaaccu ggcucgagag cagagaauac      420

acaaagcacc ucauccgggu agagaacugg aucuucagga acccaggguu cgcccuagcc      480

gcagcagcaa uagcauggcu ccuuggaagc agcaccucac agaaaguaau auaccuagua      540

augauucucc ucaucgcccc agcguacagc auaagaugca ucggagucuc caauagagac      600

uucguagaag gcaugucagg aggcacaugg guagacguag uccuagaaca cggcggaugc      660

gugaccguaa uggcacaaga caaaccaacg guggauauag agcuggucac aaccacagua      720

agcaacaugg cggaaguccg gagcuacugc uacgaggcau caaucagcga cauggcaagc      780

gacagucgau gccccaccca aggcgaagcc uaccucgaca aacaaagcga cacgcaauac      840

guaugcaaac gcacacucgu cgacaggggg uggggaaacg gcugcggacu cuucggcaaa      900

ggcucacucg uuaccugcgc aaaauucgcg ugcuccaaga aaaugaccgg caaguccauc      960

caaccugaaa accuggaaua ccgaaucaug cuaucggucc acggcagcca acacagcgga     1020

augauaguga acgauacagg ucacgaaaca gacgagaaca gagcaaaagu agaaauaacu     1080

ccaaacucac cgcgagcaga agccacccug ggcggcuucg gaagccuagg ccucgacugc     1140

gaaccccgca ccgggcuaga cuuuagcgau uuguacuacc ucaccaugaa caacaaacac     1200

uggcucgucc acaaagaaug guuccacgac auaccccucc cauggcacgc gggcgcagac     1260

accggaaccc cacacuggaa caacaaagaa gccuuaguag aguucaaaga cgcacacgca     1320

aagcgacaaa ccguagucgu gcucggcucc caagaaggag ccguacacac cgcacucgcc     1380

ggggcacugg aagcagaaau ggacggcgca aaaggaagac ucucaucagg acaccuuaaa     1440

ugccgacuca aaauggacaa acuaagauua aagggaguca gcuacagucu cugcacggcg     1500

gccuuuaccu ucacaaaaau ccccgccgaa acacuacacg gaacagucac cguggaagua     1560

caauacgcgg gaacagaugg accaugcaaa gugccagccc aaauggcagu cgauaugcaa     1620

acccuaaccc ccguaggacg auugauaaca gccaacccag ugauaacaga aagcaccgaa     1680

aacagcaaaa ugaugcuaga acuagauccc ccauucggag acagcuacau agucaucggg     1740

guaggagaaa agaaaauaac ccaccacugg caccgaagcg gcagcacacu gggcaaagcc     1800

uuuucuacga cgcucaaagg agcacaacga cucgcagcuc ucggagacac agccugggac     1860

uucggcagua uaggaggcgu auucaacucg auagggagag caguccacca aguauucgga     1920

ggagcauuca gaacacuguu cggaggcaug agcuggauaa cacaaggacu uaugggcgca     1980

cuccuacugu ggaugggagu caacgcuaga gacagaucaa uagcccuagc cuucuuggcc     2040

accggcggag uacucguauu ccuugcaaca aacgugcacg cg                        2082


<210>  9985
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9985
augacauccg ugggaauagu cggacuauua cucaccaccg caauggccgc agucacccuc       60

ccaagucacu cuacccgcaa acuccaaacc cgaucgcaga ccuggcucga aagccgcgaa      120

uacacaaaac accucauccg agucgaaaac uggaucuucc gaaacccagg auucgcccuc      180

gccgccgccg caaucgccug gcuccucggg agcucuacau cacaaaaagu cauauaccuc      240

guaaugauac uacucauagc ccccgccuau uccauccgcu guauaggagu aucaaaccga      300

gacuucguag aaggcaugag cggcggcacc uggguagaca ucguacucga acacggaggc      360

ugcguaaccg ucauggcaca agacaaaccc accguagaca uagaacucgu cacaacaacc      420

guaucaaaca uggcggaagu ccgcuccuac ugcuacgaag ccucaaucag cgacauggcc      480

uccgacucac gcugcccaac acagggcgag gccuaccuag acaaacaaag cgacacccaa      540

uacguaugca aacgcacacu cgucgaccga ggcuggggaa acggcugcgg ccucuucgga      600

aaaggaagcc ucguaaccug cgccaaauuc gccugcucca agaaaaugac uggaaaaucc      660

auacaaccgg aaaaccucga auacagaauc augcucagcg uccacggcuc acaacacagc      720

ggcaugaucg ugaacgacac cggacacgaa accgacgaaa accgcgcaaa aguagaaaua      780

acuccaaacu caccacgcgc ugaggccaca cuaggcggcu ucggcucccu agggcucgac      840

ugcgaaccaa gaaccggucu cgacuucuca gaccucuacu accucacaau gaacaacaaa      900

cacuggcuug uccacaaaga augguuccac gacauacccc uccccuggca cgccggcgcc      960

gacacaggca caccccauug gaacaacaaa gaagcccucg uggaguucaa agacgcgcac     1020

gcaaaacggc aaaccguagu agugcuaggg ucccaagaag gagccgucca cacggcccuc     1080

gcuggagccc ucgaagcaga aauggacggc gcuaaaggcc gacuguccuc aggacaccuc     1140

aaaugccgcc ucaaaaugga caaacuacgc cucaaaggug uuuccuacuc acuaugcacc     1200

gccgccuuca cguucaccaa aaucccagcc gaaacccucc acggaacagu caccgucgaa     1260

gugcaauacg ccggcaccga cggccccugc aaagucccag cccaaauggc aguggacaug     1320

caaacccuaa ccccagucgg ccgccuaauu accgccaacc ccgucaucac cgaauccaca     1380

gaaaacagca aaaugaugcu cgaacucgac ccacccuucg gcgacucgua caucgugaua     1440

ggcgucggag aaaagaaaau cacacaccac uggcaccgca gcggcucaac aauaggcaaa     1500

gcguucgaag ccaccguccg cggcgccaaa cgcauggccg ugcucggcga cacagccugg     1560

gacuucggau ccgucggugg ugcccuaaau agcuugggca agggaaucca ccaaaucuuc     1620

ggagccgccu ucaaaagccu uuucggaggg auguccuggu ucucccagau ccucauagga     1680

acccuacuaa uguggcuagg ccuaaacacg aagaacggcu caauaagccu caugugccuc     1740

gcacuaggcg gaguacuaau cuuccugucg acugccguau ccgca                     1785


<210>  9986
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9986
augaccagcg ucggaauagu cggccuccuc cucacgacag cgauggcagc cgugacacuc       60

cccucccacu cgacaagaaa acuacaaaca cgcucccaaa cguggcucga auccagagag      120

uacacuaaac accugauccg ugucgaaaac uggauauucc gcaacccagg cuucgcccug      180

gcagccgccg caaucgccug gcuacucggc agcagcaccu cacaaaaagu caucuaucua      240

guaaugauac uccugaucgc gccagcuuac uccauccgcu gcauaggagu auccaacaga      300

gacuucgucg aaggcaugag cggaggcaca ugggucgacg ucguccuaga acacggagga      360

ugcgucaccg uaauggcaca agauaaaccc acggucgaca ucgagcuagu uaccacaaca      420

gucagcaaca uggccgaagu ccgcaguuac ugcuacgaag ccagcauaag cgacauggcc      480

ucagacagca gaugcccaac ccaaggcgaa gccuaccucg acaaacaguc agacacgcaa      540

uacguaugca aaagaacccu cguagaucga ggcuggggca acggaugugg acucuucggg      600

aaaggcagcc ucguaaccug cgccaaauuc gcgugcagua agaaaaugac aggcaaaucc      660

auccaaccag aaaaccucga auaccguauu augcucuccg uccacggcag ccagcacucc      720

ggcaugaucg uaaacgacac cggacacgag accgacgaaa accgagccaa agucgaaauc      780

acccccaacu caccccgcgc agaagccaca cucggcggcu ucggcucccu cggacuggac      840

ugcgaaccaa ggacaggccu agacuucuca gaccuauacu accucacaau gaacaacaaa      900

cacuggcucg ugcacaaaga augguuccac gacauccccc uccccuggca cgccggagca      960

gacaccggaa caccccacug gaacaacaaa gaggcccuug uagaguucaa agaugcacac     1020

gccaagcgcc aaaccguagu uguacuggga agccaagaag gcgcaguaca caccgcacua     1080

gcaggagcac uagaagccga aauggacggc gccaaaggcc gccuauccag cggccaccua     1140

aaaugccgcc ucaaaaugga caagcucagg cugaaaggcg ucagcuacuc ccucugcacc     1200

gcagcauuca ccuucaccaa aauaccugca gaaacccucc acggcaccgu caccgucgaa     1260

guacaauacg caggcacgga cggcccaugc aaaguacccg cccaaauggc cguggacaug     1320

caaacccuca caccaguagg ccgacucauc acagccaauc caguaaucac ggaaagcaca     1380

gaaaacagua aaaugaugcu agaacucgac ccacccuucg gagacucuua caucgucaua     1440

ggaguaggcg aaaagaaaau cacccaccac uggcaucgcu ccggcucaac caucggcaaa     1500

gcauucgaag ccaccguacg cggcgccaaa cguauggcug uccuaggaga caccgcaugg     1560

gacuucggau cggucggcgg cgcccucaac agccuaggaa aaggcaucca ccaaaucuuu     1620

ggagcggcgu ucaaaucccu cuucggagga augucguggu ucucccaaau acuaaucggu     1680

acgcuacuaa uguggcucgg ccucaacgcc aaaaacggau caauuagccu caugugccua     1740

gcacuaggcg gaguccuaau cuuccuaagc acagcgguaa gcgcc                     1785


<210>  9987
<211>  1773
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9987
augaccucca ucggaauaau uggccuacua cucacaaccg caauggcggc cguaacccuu       60

cccucacacu ccacucguaa acugcaaacc cgcucccaga cauggcucga aucgagagaa      120

uacacaaaac accuaaucaa agucgaaaac uggaucuucc gcaaccccgg auucgcccua      180

gucgccgucg caaucgcaug gcuccucggc uccucaacca gccaaaaagu uaucuaccuc      240

guaaugaucc uccucaucgc acccgcauac ucaauacgcu gcaucggcgu cucuaaccgc      300

gacuuuguag aaggaauguc aggcggcacc ugggucgacg ucguacucga acacggggga      360

ugcgucaccg ucauggccca agacaaaccu acagucgaca ucgagcuagu cacgaccaca      420

gucucaaaca uggcagaggu aagaagcuac ugcuacgaag ccuccaucuc cgauauggcc      480

uccgacuccc gaugccccac acagggcgaa gcauaccuag acaaacaauc cgacacccaa      540

uacgucugca aacgcacacu cguagaccgu ggcuggggga acggcugcgg acuauucgga      600

aagggcuccc ucgucacaug cgcgaaauuu accugcucca agaaaaugac cggaaaaagc      660

auacagcccg aaaaccucga auaccgcauc augcucuccg ugcacggcag ucaacacuca      720

ggcaugauag gcuacgaaac agaugaagac cgcgcaaagg ucgaagucac cccaaacucc      780

cccagagccg aagccacccu cgggggcuuc ggcagccucg gacuagacug cgaaccuaga      840

accggccuag acuucagcga ccucuacuac cuaacgauga acaacaaaca cuggcuugua      900

cauaaagaau gguuccacga caucccccug cccuggcacg ccggagccga cacaggaaca      960

ccgcacugga acaacaaaga agcacucguu gaguucaaag acgcccacgc aaaacgccaa     1020

acagucgucg uacuaggaag ccaagaaggc gccguccaca ccgcacuagc aggagcauua     1080

gaagccgaaa uggacggcgc aaaaggccgc cucuucuccg gccaccucaa augccgacua     1140

aaaauggaua aacuccgccu caaaggcguc agcuacucac ucugcaccgc cgccuucaca     1200

uucaccaaag uccccgccga aacacuacac ggcaccguaa cugucgaggu acaauacgcc     1260

ggaacggacg gcccuugcaa gauacccgua caaauggcag uggacaugca aacacucacc     1320

cccgucggca gacuaauaac agccaacccg guaauaaccg aaagcaccga gaacucuaaa     1380

augaugcuag aacucgaccc acccuucggc gacagcuaca ucgucaucgg cgucggcgac     1440

aagaagauca cacaccacug gcacagauca gguuccacaa ucggcaaggc auucgaagcc     1500

acgguccgcg gcgcaaaacg aauggccguc cuaggcgaua ccgccuggga cuucggcucc     1560

gucggaggag ucuucaacuc ccucggaaaa ggaauccacc aaaucuuugg agccgccuuc     1620

aaaucgcuau ucggcggcau gagcugguuc agccagaucc uaauagguac acugcucgua     1680

uggcucggcu uaaauaccaa aaacggaagc aucucccuaa cgugccucgc ccucggcggc     1740

gucaugaucu uccucuccac agccgugagc gca                                  1773


<210>  9988
<211>  1785
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9988
augacaagcg uaggcauagu cgggcuccua cuuacaacag ccauggcagc cguaacauua       60

ccaucacacu ccacccgcaa acuacagacc agaucccaaa ccuggcucga aagccgcgaa      120

uacacaaaac accucauacg cgucgaaaac uggaucuuca gaaaccccgg guuugcccua      180

gcagccgcag caauagccug gcugcuggga agcuccacau cacaaaaggu caucuaccua      240

guaaugaucc ugcucauagc cccagccuac uccauccgcu gcauaggcgu cagcaaccgc      300

gacuucguag aagggauguc aggcgguaca uggguagacg ucguacucga acacggagga      360

ugcgucaccg uaauggcgca ggacaaacca acaguagaca ucgaacucgu cacaaccacc      420

gucucgaaca uggccgaagu gcgaagcuac ugcuaugaag cauccaucuc agacauggcg      480

uccgacagcc gaugccccac ccaaggagag gcauaucucg acaaacaaag cgacacccaa      540

uaugugugca aacgcacccu cguggaccgc ggauggggaa acggaugcgg ccuguucgga      600

aaaggcucac ucguaacaug cgcaaaauuc gcaugcagca agaaaaugac cggaaaaagc      660

auacagccug aaaaccuaga auacagaaua augcugagcg uccacggcuc acaacacucc      720

ggcaugauag uaaacgacac aggccacgaa accgacgaaa acagagccaa aguagaaauc      780

acaccaaauu ccccacgagc agaagccaca cucggcggcu ucggcagccu aggccuagac      840

ugcgaaccac ggacaggacu agacuucagc gaccucuacu accucacaau gaacaacaaa      900

cacuggcugg uccacaaaga augguuccac gauauacccc uccccuggca cgccggagca      960

gacaccggca ccccacacug gaacaacaaa gaagcacugg ucgaguucaa agacgcucac     1020

gcgaaacgcc agacagucgu cguacugggc agccaagaag gcgccgucca caccgcucuc     1080

gccggcgcac uagaagcgga aauggacggg gcaaaaggcc ggcuaucaag cggccaccug     1140

aaaugccgac ucaaaaugga caaacuacga cugaagggcg ucagcuacag ucuaugcaca     1200

gccgcauuca ccuucacaaa aauccccgca gaaacccuac acggcacagu caccgucgaa     1260

gugcaauacg ccggcacaga cggacccugc aaaguccccg cucagauggc cgucgacaug     1320

caaacauuaa cccccgucgg acgacuaaua accgcaaacc caguaaucac cgaauccaca     1380

gagaacucca aaaugaugcu cgaacucgau cccccauucg gcgacagcua cauaguaauc     1440

ggcguaggag aaaagaaaau aacgcaccac uggcaccgaa gcggcuccac cauaggcaaa     1500

gcauucgaag cgaccguccg cggagccaaa cgcauggcag ucuuaggcga caccgcaugg     1560

gacuucggcu ccgucggcgg cgcccuaaac agccucggaa aaggaaucca ccaaaucuuc     1620

ggcgccgcau ucaaaagccu auucgggggc augucauggu ucucccaaau ccugauagga     1680

acccuacuca uguggcuagg gcugaacacc aaaaacggca gcauaagccu aaugugccua     1740

gcacuaggcg gaguccucau auuucucuca acagccgucu cggcc                     1785


<210>  9989
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9989
auggccauau caggcguccc uguccucggc uucuucauca ucgccguacu aauguccgcc       60

caagagucau gggccgcagu gacccuccca agccacagca cgcgcaaacu acaaacccgc      120

agccagaccu ggcucgaaag ccgagaauac accaaacacc uaauaagagu cgagaacugg      180

aucuuccgca accccggauu cgcgcucgcg gccgccgcca ucgcauggcu ccuaggcagc      240

uccaccucac aaaaagucau auaccucgug augauccucc uuaucgcccc cgccuacucc      300

auccgaugca ucggagucag caaccgagac uucgucgaag gaaugucagg cggcacaugg      360

gucgacauag uccucgaaca cggcggaugc gucaccguaa uggcccaaga caaacccaca      420

gucgacaucg agcuagucac aaccacagua ucaaacaugg cagaaguaag aagcuacugc      480

uacgaagccu ccaucucaga cauggccucg gacucacgau gccccacgca gggagaagcc      540

uaccuggaca aacagucuga cacacaauac gucugcaaaa gaacccucgu agaccgcgga      600

uggggcaacg gcugcggacu auucggaaag ggcucccuag uaaccugcgc aaaauucgca      660

ugcucgaaga aaaugaccgg caaaucaauu caacccgaga acuuagaaua ccgcauaaug      720

cucucaguac acggcucaca acauagcggg augaucguua acgacaccgg ccacgaaaca      780

gacgaaaacc gagcgaaagu cgaaauaaca ccaaacagcc cacgcgcgga agccacccug      840

ggcggcuucg ggucccucgg cuuagacugc gaaccgcgaa caggacuaga cuuuagugac      900

cucuacuacc ucaccaugaa caacaaacac ugguuaguac auaaagaaug guuccacgac      960

aucccacugc ccuggcacgc cggcgccgac accggcacac cccacuggaa caauaaagaa     1020

gcccucguag aguucaaaga cgcccacgcg aaacgacaaa cagucgucgu acuaggcagc     1080

caagaaggcg cuguccacac cgcucucgcc ggagcacucg aagccgaaau ggauggagcg     1140

aaaggacgac uaagcuccgg ccaccucaag ugccgacuca aaauggacaa gcuccgccug     1200

aaaggcguca guuacucccu uugcacugca gcauuuaccu uuacgaaaau acccgccgaa     1260

acucuacacg gaaccgucac aguagaaguc caauacgccg gcacagacgg accaugcaag     1320

gucccagccc aaauggccgu cgacaugcaa acacuuaccc caguaggaag acuaauaaca     1380

gccaaucccg uaaucacaga aucuaccgag aacucaaaaa ugaugcucga gcucgauccc     1440

ccauuuggcg acagcuacau cgucauaggg gucggcgaaa agaaaaucac acaccacugg     1500

caccgcagcg gcagcacaau aggcaaagca uucgaagcca cagucagagg cgcaaaacgu     1560

auggcgguac ugggcgacac agccugggac uucggaagug uaggcggagc acuaaacucg     1620

uuaggcaaag gcauacacca aauauucgga gcagccuuca aaagccuguu cggcggaaug     1680

ucaugguucu cgcaaauccu aaucggcacu cuccucaugu gguuaggccu caacaccaaa     1740

aacgggagca ucagccuaau gugccucgca cucggaggag ugcucaucuu ccuaagcacg     1800

gcagucuccg cc                                                         1812


<210>  9990
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9990
auggccauca gcggcguccc cguacucgga uucuucauca ucgccguacu aauguccgcc       60

caagaaagcu gggcagccgu gaccuuacca ucacacucaa cuagaaaacu ccaaacacgc      120

ucccaaaccu ggcucgaauc uagagaguac accaaacacc ucauccgagu agaaaacugg      180

aucuuccgaa acccaggauu cgcacuugcc gcagccgcga ucgcauggcu gcucggaagc      240

ucuaccucac aaaaaguaau auaccuaguc augauucucc uuaucgcccc cgcauacagc      300

auacgcugca ucggagucuc caacagagac uucgucgaag gcaugagcgg cggcacaugg      360

guagacguag uccucgaaca cggaggaugc gucaccguaa uggcccaaga caaacccacc      420

guagacauag aacuggucac aacaaccguc ucaaacaugg ccgagguaag gagcuacugc      480

uacgaagccu ccaucuccga cauggcaagc gacagucgcu gcccaaccca aggagaagcc      540

uaccuagaca agcaaaguga cacccaauac guaugcaaac gaacccucgu cgaccgagga      600

uggggaaacg ggugcggccu auucggaaaa ggaagccucg ucacgugcgc aaaauucgca      660

ugcagcaaga aaaugacagg caaaagcauc caacccgaaa accucgagua cagaauaaug      720

cuaagcgugc acggcucaca acacuccggc augaucguaa acgacacagg acacgaaacc      780

gacgagaacc gagccaaagu cgaaaucacc cccaacucac cacgcgccga agcaacccuc      840

ggcggauuug guucccucgg acuagacugc gaaccaagaa ccggacucga cuucuccgac      900

uuauacuacc uaaccaugaa caacaaacac uggcuaguac auaaagaaug guuccacgac      960

aucccacuac ccuggcacgc gggcgcggac accggcaccc cacacuggaa caacaaagaa     1020

gcacucguag aauuuaaaga cgcacacgcc aaacgccaaa cagugguagu gcuaggaucc     1080

caggaaggag ccguccacac cgcacucgca ggggcccuug aagccgaaau ggacggagcg     1140

aaaggccgcc uuucgagcgg acaccucaag ugccgccuaa agauggacaa acuccgacuc     1200

aagggaguaa gcuacucccu cugcacagca gcauucacau ucacaaaaau acccgccgag     1260

acacuucacg gcaccgucac cgucgaaguc caguaugccg gaaccgacgg acccugcaaa     1320

guaccagcuc aaauggccgu agacaugcaa acccuaacgc ccgucggacg acuaauaaca     1380

gcgaacccgg ucaucaccga aucaaccgaa aacagcaaaa ugaugcucga acuagauccc     1440

ccauucggag acagcuacau aguaaucggc guaggagaaa agaaaaucac gcaccacugg     1500

caccgcucag gaucaaccau aggaaaagcg uucgaagcaa ccgugcgcgg cgcuaaacgc     1560

auggcagucc ucggcgacac cgccugggac uuuggaucag ucggaggagc ccucaacagc     1620

cucggcaaag gcauacacca aaucuucgga gcagccuuua aaucccucuu cggaggcaug     1680

ucgugguuca gccaaauacu aaucgggacc cuacuaaugu ggcuaggacu caacgccaaa     1740

aacggcagca uaucccucau gugccuagcc cucggcggag uccucauauu ucuaucaaca     1800

gccgucucag cu                                                         1812


<210>  9991
<211>  1800
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9991
auggcgauau ccggaguccc aguccucggc uucuucauca ucgcaguccu gaugucagcg       60

caagaaagcu gggcagcggu cacacuaccc agccacucca cacgcaaacu ccaaacacgc      120

agccagacau ggcuagaauc ccgcgaauac acaaaacacc ucaucaaggu agaaaacugg      180

aucuuccgaa accccggauu cgcgcucguc gcuguagcaa ucgcauggcu acucggcagc      240

ucgacaucgc aaaaagucau cuaccuaguc augauccuac ucaucgcacc cgccuacucc      300

auaagaugca ucggcgucuc caaccgagac uucguagaag gaaugucagg cggcaccugg      360

gucgacgucg uccuagagca cggaggcugc guaaccguca uggcacagga caaaccaaca      420

gucgacauag aacucguaac aaccaccgua ucaaacaugg ccgaaguccg uucauacugu      480

uacgaagccu caauaaguga cauggccuca gacagccgau gccccaccca gggggaggca      540

uaccuggaca agcaaucaga cacccaauac gucugcaaaa gaacgcucgu agaccgcggc      600

ugggguaacg gcugcgggcu auucggcaaa ggcucccucg ucacgugcgc caaauucacg      660

ugcagcaaga aaaugaccgg aaaaagcauc caaccugaaa accucgaaua ccgaauaaug      720

cuguccgucc acggcagcca acacagcggc augaucggcu acgaaaccga cgaagaccgc      780

gcaaaagucg aagucacacc caacucacca cgcgcggaag ccacacuggg aggauucgga      840

agccuaggac uagacugcga accacgaaca ggacucgacu ucagcgaccu uuacuaccuc      900

accaugaaca acaaacacug guuaguccac aaagaauggu uccacgacau cccccucccc      960

uggcacgccg gagccgacac aggaacgccc cacuggaaca acaaagaagc ccucgucgag     1020

uucaaagacg cgcacgcaaa aaggcaaacc gucgucguuc uaggaucaca agaaggcgcg     1080

guacacaccg cgcuagccgg cgcccuagaa gccgaaaugg acggugccaa aggucgccuc     1140

uucucaggac accucaaaug ccggcugaaa auggacaaac uccgccuaaa aggcguaucc     1200

uacagccucu gcaccgcagc cuucaccuuc acaaaagucc cugccgaaac ccugcacggc     1260

accgucaccg ucgaagucca auacgccgga acggauggcc ccuguaaaau ccccguacaa     1320

auggccguag acaugcaaac ccucacgccc guaggccgac ucaucaccgc caacccaguc     1380

auaacagaau ccaccgaaaa uagcaagaug augcuagaac ucgauccccc auucggagac     1440

ucauacauag ucaucggagu cggcgacaaa aagauaacac accacuggca ccgcucaggc     1500

ucaaccaucg gcaaagccuu cgaagccacc guccgcggcg caaaaagaau ggcaguccuc     1560

ggagacaccg caugggacuu cggcuccgua ggaggcguau ucaauagccu aggcaagggc     1620

auucaccaaa uauucggggc agccuucaag agcuuauucg gcgggauguc augguucucc     1680

caaauacuca ucggaacacu ccuagugugg cucggccuca acacaaagaa cggcucaauc     1740

agccuuaccu gccucgcacu cggaggcgug augauauucc uauccacagc cguauccgcc     1800


<210>  9992
<211>  1812
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9992
auggcaauau ccggcgugcc cguacucgga uucuucauca ucgccguccu caugucagcg       60

caagaauccu gggcagccgu aacacucccc ucccacagca cccgcaaacu acaaacaaga      120

agccaaaccu ggcuagaauc acgagaauac accaagcacc uuauaagagu cgaaaacugg      180

auauuccgaa acccaggauu cgcacuggcg gccgcagcaa uagcauggcu ccuaggcucg      240

aguacaagcc aaaaaguaau cuaccucgua augauccuac uaaucgcacc cgcguacuca      300

aucagaugca ucggcgucag caacagagac uucguagaag gaauguccgg agggacuugg      360

gucgacguag uacuagaaca cggcggcugc gucacaguaa uggcacaaga caaaccaacc      420

gucgacauag agcuagugac cacaaccguc agcaacaugg cggaaguacg cagcuacugc      480

uacgaagcaa gcauaucgga cauggcaagc gacagccgcu gcccaacaca gggcgaagca      540

uaccuagaca aacaauccga cacccaauac guuugcaagc gaacacucgu cgaccgcggc      600

uggggcaacg gcugcggccu auucggcaaa ggcucccuag uaacaugcgc aaaauucgca      660

ugcucuaaga agaugacagg caagagcaua caaccagaaa accucgaaua caggaucaug      720

cuaucggucc acgguucaca acauucaggc augaucguca augacacggg acacgaaacc      780

gacgaaaacc gcgccaaggu ggaaauaacg cccaacaguc cccgcgccga ggcaacccug      840

ggcgguuucg gcagccucgg ccucgacugc gaaccaagaa ccggacucga cuucagcgac      900

cuauacuacc uaacuaugaa caacaagcac uggcuugucc acaaagagug guuccacgac      960

auaccccucc ccuggcacgc aggagcagac accggaaccc cacacuggaa caacaaagaa     1020

gcacucguag aguucaaaga cgcccacgcg aaaagacaaa cagucguggu acuggggagc     1080

caagaaggug ccguccacac agcccuagcc ggagcacucg aagcagaaau ggacggcgca     1140

aaagggcgcc ucucaucagg ccaccuaaag ugccgacuaa aaauggacaa acuccgccua     1200

aaaggcguau cuuacucccu augcaccgcc gcauucaccu uuacaaagau accggccgaa     1260

acccuccacg gcaccguaac aguagaaguc caauacgccg gcaccgacgg ccccugcaaa     1320

guccccgccc aaauggcagu agacaugcaa acacuaacac ccgucggccg acucauaacc     1380

gccaaccccg ucauaacaga aaguacugaa aacuccaaaa ugaugcucga acucgaccca     1440

cccuucggag acagcuacau agucauaggc gucggcgaga aaaagauaac ccaccacugg     1500

caccgcuccg gcuccacaau aggcaaggcc uucgaagcca ccguaagagg cgcaaaacgc     1560

auggcagucc ugggagacac agcaugggac uucggcuccg uuggaggggc acuaaacuca     1620

cuagggaaag gcauacauca aauuuucggc gcagccuuca aaagccuauu cggcggaaug     1680

agcugguuca gccaaauacu aaucggcaca cuacuuaugu ggcucggccu caacacgaaa     1740

aacggaagca ucucccuaau gugccuagca cucggcggcg ugcuaauauu ccuaagcaca     1800

gcagucagcg ca                                                         1812


<210>  9993
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9993
auggacugga cauggauacu auuccucgua gcagcagcga caagagugca cagugcagua       60

acccuccccu cacacagcac gagaaaacua cagacgcgca gccaaaccug gcucgaauca      120

cgagaauaca ccaaacaccu aauacgaguc gagaacugga uauucagaaa ccccggcuuc      180

gcccuagcag cagcagcaau cgccuggcuc cuaggcagca gcacaucaca aaaagugaua      240

uaccugguaa ugauccuucu uauagccccc gccuauagua uaagaugcau cggcgucagc      300

aaucgcgacu ucgucgaagg aaugucggga ggaaccuggg ucgacauagu ucuagagcac      360

ggaggcugcg ucaccguaau ggcacaagac aaaccaacag uggacaucga auuaguuacc      420

acaacgguaa gcaacauggc cgaagucaga agcuauugcu acgaagcaag cauaucagac      480

auggcaagcg acucccgaug ccccacccaa ggcgaggcuu accuagacaa acagagugac      540

acccaguacg ugugcaaaag aacccuaguc gaccguggcu ggggcaacgg augcggacuc      600

uucggcaaag ggagccucgu aacaugcgca aaauucgcuu gcucaaagaa aaugaccggc      660

aaaagcaucc aaccagaaaa ccuagaauac cgaaucaugc ucuccguaca cggaucacaa      720

cacagcggaa ugauagugaa cgacaccggg cacgagacag acgagaaccg cgccaaagua      780

gaaauaaccc ccaacagucc cagagccgaa gcgacacuag gaggauucgg aucauuaggg      840

cuggacugcg agccacgaac cggccuagac uucucagacc uauacuaccu cacgaugaac      900

aacaaacacu ggcucguaca caaggaaugg uuccacgaca uaccccuacc auggcacgcc      960

ggcgcagaca caggcacacc ccacuggaac aauaaagaag cccucgucga auuuaaagac     1020

gcccacgcca aacgccaaac agugguggua cucggcucac aagaaggugc cguucacacg     1080

gcccuagcag gcgcccugga agccgaaaug gacggagcca aaggucgacu aagcagcgga     1140

caucuaaaau gcagacugaa aauggacaaa cuccggcuca aaggaguauc auacagccua     1200

ugcaccgccg ccuucacguu cacaaaaaua ccagcagaga cccuccacgg cacagucacg     1260

guagaagucc aauacgccgg caccgacggc cccugcaaag ugccagcaca aauggcaguc     1320

gacaugcaaa cccugacacc cgucggcagg cuaaucaccg caaaccccgu caucacagag     1380

aguacagaga acagcaaaau gaugcucgaa cucgacccac ccuucggaga cucguacauc     1440

gucauaggcg ucggcgaaaa gaaaaucacc caucacuggc auagaagcgg aagcaccaua     1500

ggcaaagccu ucgaagccac cguacgcggg gccaaacgca uggccguccu cggagacaca     1560

gcgugggacu ucggcucggu aggcggcgca cucaacucac ucggcaaagg cauacaccaa     1620

aucuucggcg cagccuucaa aucccuauuc ggcgggauga gcugguucuc gcaaauacua     1680

auaggcaccc ugcuaaugug gcucggacua aacaccaaaa acggcucgau cucauuaaug     1740

ugccuagcgc ucggcggagu acuaaucuuc cuaucaacag caguaucagc c              1791


<210>  9994
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9994
auggacugga ccuggauccu cuuccucgua gccgccgcca cacgcguaca cagcgcaguc       60

acacucccga gccacagcac cagaaaguua caaacacgcu cucaaacgug gcuagagucc      120

cgagaauaca caaaacaccu aauacgcgua gaaaacugga ucuucagaaa cccagguuuc      180

gcccuggcgg cagccgccau cgcguggcua cucggcucaa gcaccagcca aaaagucauc      240

uaccucguca ugauccuacu cauagcccca gccuacucca uccgaugcau cggagucucc      300

aauagagacu ucguggaggg aaugagcggc gguaccuggg ucgacguugu ccuagaacac      360

ggcggcugcg ucaccguuau ggcccaagac aaacccaccg ucgacauaga auuagucacc      420

acaacagucu caaacauggc cgaaguccga agcuacuguu acgaagccuc caucuccgac      480

auggccagcg acucacggug cccgacccaa ggcgaggcuu accucgacaa acaaucagac      540

acacaauacg ucugcaaacg cacacucguc gacagaggcu ggggcaacgg cugcggccug      600

uucggcaaag gcucccuagu uaccugcgcu aaauucgcau gcagcaagaa aaugaccggc      660

aaaagcaucc agcccgaaaa ccuagaauac agaaucaugc ucucagucca cggaagccaa      720

cacagcggca ugauagucaa cgacaccggc cacgaaaccg acgaaaacag agccaaaguc      780

gaaaucaccc ccaacucacc ccgcgcggag gccacacuug gcggauucgg cucccuagga      840

cuggacugcg aaccccgcac cgggcuagac uucuccgacc uauacuaccu cacaaugaac      900

aacaaacacu ggcuagucca caaagaaugg uuccacgaca uaccauuacc cuggcaugcc      960

ggcgcagaca caggaacacc ccacuggaac aacaaagaag cacucgucga guucaaagac     1020

gcccacgcca aacgccaaac cgucgucgug cuaggauccc aagaaggugc cguacacacc     1080

gcccuugcag gggcucucga agccgaaaug gacggcgcca agggacgccu uuccuccggc     1140

caccugaaau gccggcucaa aauggacaaa uuacgacuca aggguguauc auacucccuc     1200

ugcaccgcag cauucaccuu caccaaaauc cccgccgaaa cgcuccaugg caccgucaca     1260

gucgaaguuc aauacgccgg caccgacgga cccugcaagg ugccggcgca aauggccguc     1320

gacaugcaaa cccuaacgcc cguagggagg cucaucacug ccaacccagu aauaacagaa     1380

agcacagaaa acuccaaaau gaugcucgaa cucgacccgc ccuucgggga cucauacaua     1440

guaauuggcg uaggcgaaaa gaaaauaaca caccacuggc acagaagcgg aucaacaaua     1500

ggaaaagccu ucgaagcaac gguaagaggg gccaaaagaa uggccguacu cggagacacc     1560

gccugggacu ucggcucagu ugggggugca cuaaacuccc ucggcaaagg uauccaccaa     1620

auauucggcg cugcauucaa aucacucuuc ggaggaaugu caugguucuc ccagauacuc     1680

auaggcaccc ugcucaugug gcucggccuc aacgcgaaaa acgguagcau uucacuaaug     1740

ugccucgcau uaggcggagu gcucauauuc cuaucaaccg cagucagcgc c              1791


<210>  9995
<211>  1779
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9995
auggauugga ccuggauacu cuuccuaguc gcagcagcaa ccagaguaca cucagccguu       60

acccucccca gccacucaac aagaaagcug caaacacgca gccaaacgug gcuagaaucc      120

cgcgaauaca cgaaacaccu cauaaagguc gaaaacugga ucuucagaaa cccaggcuuc      180

gcgcucgucg cggucgccau cgcauggcua cucgggucca gcacuuccca aaaagucaua      240

uaccuaguca ugauccuacu caucgcaccc gcuuacagca uccgcugcau cggaguaucc      300

aaucgagacu ucguagaagg caugagcgga ggcacauggg uagacguagu ccucgaacac      360

ggcggaugcg ucaccguaau ggcgcaagac aaacccaccg uagacaucga acucguaaca      420

acaaccgucu cgaacauggc agaagucaga agcuacugcu acgaagcaag cauaagcgac      480

auggcgagcg acucccgcug ccccacccaa ggggaagccu accuggacaa acaaagcgac      540

acccaauacg uaugcaaacg aacacucgua gauagaggcu ggggcaacgg augcggccua      600

uucggaaagg gaucccuagu cacaugcgca aaauucacau gcucaaagaa aaugacaggc      660

aaaucaauac aacccgaaaa ccucgaauac cgcaucaugc ucagcgucca cggaucacaa      720

cacucgggaa ugauaggaua cgaaacggac gaagaccggg caaaaguaga ggucacacca      780

aacuccccaa gggcggaagc cacccucggc gguuucggcu cacuggggcu agacugcgaa      840

ccccgcaccg gacucgauuu cucagaccua uacuaccuca caaugaacaa caaacacugg      900

cucgugcaca aggaaugguu ccacgacaua ccccucccgu ggcacgccgg cgcggacaca      960

ggaacacccc acuggaacaa uaaagaagca cucgucgagu ucaaagacgc acacgccaaa     1020

cgccagaccg uaguaguacu cggaucacaa gaaggggccg uacacacggc gcuggccgga     1080

gcacuagaag cagaaaugga uggcgcaaaa ggccggcuau ucaguggaca ccuaaaaugu     1140

cgccuaaaaa uggacaaacu cagacucaaa ggagucuccu acucccuaug cacggccgcg     1200

uucacauuca caaaaguccc cgcagaaaca cuccacggca cgguuacagu cgaaguccaa     1260

uacgcaggca ccgacggccc cuguaaaaua ccuguacaaa uggccgucga caugcaaaca     1320

cucacacccg ucggaagacu gaucacugca aacccaguca uaacagaaag uacagaaaac     1380

agcaaaauga ugcuagaacu ugacccaccc uucggcgauu cguacaucgu caucggcgua     1440

ggagacaaga aaaucaccca ccacuggcac cgaagcggca gcaccauagg aaaagcauuc     1500

gaagcaacag uacgcggagc caaaagaaug gccguccuug gagacacagc cugggacuuc     1560

ggguccgucg gaggcguauu caacagccug ggaaaaggca uccaccagau cuucggcgca     1620

gcauucaaau cgcuauucgg uggaaugucc ugguucagcc aaauccuaau cgggacccuc     1680

uuaguguggc uaggacuaaa cacgaaaaac ggcagcauca gccuaaccug ccuagcccug     1740

ggagggguca ugaucuuccu cucaacugca gucucagca                            1779


<210>  9996
<211>  1791
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  9996
auggacugga cguggauacu cuuccucgua gcagcggcca caagagucca cagcgcagua       60

acacugcccu cacacucuac ccgcaaacug caaacacgca gccaaacaug gcuagaauca      120

cgcgaguaca caaaacaccu gauccgaguc gaaaacugga uauucagaaa cccaggauuc      180

gcccuggcag cggccgccau agccuggcua cucggaagcu cuaccucaca aaagguaaua      240

uaccuaguaa ugauccuccu caucgccccc gccuacucaa uacgcugcau cggaguaagc      300

aaccgcgacu ucgucgaagg aaugagcgga ggaaccuggg uggacgucgu cuuagagcac      360

ggaggaugug ucacgguaau ggcccaagac aaacccaccg ucgacauaga guuggucacc      420

acaacagucu ccaacauggc cgaggucaga agcuacuguu acgaagcaag uaucucagac      480

auggccagcg acagccgcug cccaacacaa ggagaagcau accucgacaa gcaaagugac      540

acacaauacg ugugcaagcg cacgcuagua gaccgaggcu ggggaaacgg cugcggccuc      600

uucggaaaag gcagccucgu aacaugcgcc aaauucgccu gcuccaaaaa gaugaccggc      660

aagucgaucc aacccgaaaa ccucgaauac agaaucaugc uauccguaca cggaagccaa      720

cacucaggca ugauagucaa cgacacugga cacgaaaccg acgaaaaccg cgccaaagua      780

gaaaucacac caaacucacc acgagcagaa gcaaccuuag gaggauucgg cucccuagga      840

cucgauugcg agcccagaac agggcuggac uucucagacc uauacuaccu aacaaugaac      900

aacaaacacu ggcuaguaca caaagaaugg uuucacgaca uaccacuacc auggcacgcu      960

ggugcagaca cgggaacgcc ccacuggaac aacaaagaag cacucguaga guucaaagac     1020

gcacacgcaa agagacaaac aguugucguc cuaggaagcc aagaaggcgc cguacacacg     1080

gcccucgccg gagcucucga agccgaaaug gacggagcua agggaagacu cagcagcggc     1140

caccuaaaau gccgacuaaa aauggacaaa cuccgacuca agggaguaag cuacucacua     1200

ugcacggccg ccuucacauu cacaaaaaua cccgccgaga cccuccacgg aaccgucacc     1260

gucgagguac aguacgcagg aaccgacgga cccugcaaag uacccgcgca gauggcagua     1320

gacaugcaaa cacucacccc cgugggacgc cucauaaccg cgaacccagu cauaaccgag     1380

uccacggaaa acagcaaaau gaugcuagaa cuugacccac cauucggaga cuccuacauc     1440

guaauaggcg ucggcgaaaa gaaaaucaca caccacuggc accgaucagg cucgaccauc     1500

ggaaaagccu ucgaagcaac aguacgcggc gccaaacgca uggcaguccu cggagacacg     1560

gccugggacu ucggcucagu agggggcgca cucaacaguc uaggaaaagg cauacaccag     1620

aucuuugggg cagccuucaa aagccuauuc ggcggaauga gcugguucag ccaaaucuua     1680

aucggaacac uacuaaugug gcucggccua aacaccaaaa acggauccau cagccuuaug     1740

ugccuugccu uaggaggcgu gcuaauauuc cugagcacag cuguaagcgc a              1791


<210>  9997
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant CDS opt

<400>  9997
auggguaaaa gauccgccgg cagcauaaug uggcuggccu cccucgccgu cgugauagca       60

ugcgcaggcg ccgcagucac acuccccagc cacagcacca gaaaacuaca aacccgcucc      120

caaaccuggc uagaaucaag agaauauaca aaacaccuca uaagagucga aaacuggaua      180

uuccgaaacc ccggcuucgc acucgccgcc gcagcaauag ccuggcuccu cggcucuagc      240

acgucacaaa aggucauaua ucuagucaug aucuugcuaa ucgccccagc auacuccauc      300

cggugcauag gcguuuccaa ucgggacuuc guagaaggca uguccggcgg aaccuggguc      360

gauauagucc uagaacaugg cggaugcguc acagucaugg cacaagacaa acccaccgua      420

gacauagagc uagucaccac gaccgugagc aacauggccg aagucagauc auacugcuac      480

gaagcaucaa ucucggacau ggcauccgac agccgcuguc ccacacaggg agaagccuac      540

cuagacaaac aauccgacac acaauacguc ugcaaacgaa cccuaguaga ccgcgguugg      600

ggaaacggau gcggccucuu cggcaaagga agccugguca caugcgcaaa auucgccugc      660

agcaagaaga ugaccggcaa aagcauccaa ccugaaaacc ucgaauaccg caucaugcuu      720

uccguacacg gcagucaaca cuccggaaug aucgucaacg acacagggca cgaaaccgac      780

gaaaaccgcg ccaaagucga aaucacaccc aacagccccc gagccgaagc cacccucggc      840

ggcuucggcu cccucggacu agacugcgag cccaggacgg gccucgacuu cuccgaccua      900

uacuaccuca caaugaacaa caaacacugg cuaguccaca aagaaugguu ccacgacauc      960

ccccuccccu ggcacgcagg cgccgacaca ggcacaccac acuggaacaa caaggaagca     1020

cucguagagu ucaaagacgc ccacgcaaaa cgccaaacag uaguaguacu gggaucccaa     1080

gaaggagccg ugcacaccgc acuagcaggc gcacucgaag ccgaaaugga cggagcgaag     1140

ggccgacuau cguccggaca ccuuaaaugc aggcugaaga uggacaaacu cagacuaaaa     1200

ggaguauccu acucccuaug cacagccgca uucacauuca ccaaaauacc cgcagaaacc     1260

cuacacggaa ccgucacagu cgaaguccaa uacgccggaa ccgacggccc cugcaaaguc     1320

cccgcgcaaa uggccguaga caugcaaacu cucacccccg uuggaagacu caucaccgcc     1380

aacccgguca ucacggaaag uaccgaaaac uccaaaauga ugcucgagcu agacccaccc     1440

uucggagauu ccuacauagu caucggcgua ggagaaaaga aaauaacaca ucacuggcac     1500

agauccggcu ccaccauagg caaagccuuc gaagccaccg uacgcggggc uaaacgcaug     1560

gccguccucg gcgacacagc augggacuuc ggauccguag gcggagcccu caacucacug     1620

ggcaaaggga uucaccaaau cuucggcgcc gccuucaaaa gccuauucgg cggaaugagc     1680

ugguucuccc aaauccuaau uggcacccua cuaauguggc ucggccucaa cacgaaaaac     1740

ggcagcauca gccuuaugug cuuagcccuc ggaggcgucc ucaucuuccu cuccacagcc     1800

gucuccgca                                                             1809


<210>  9998
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant CDS opt

<400>  9998
auggggaaac gaagcgcagg cuccaucaug uggcucgccu cacuagcagu cgucauagca       60

ugcgcaggcg cagccguaac ccucccuucc cacucaacaa gaaaacuaca aaccagauca      120

caaacguggc uagaaucgcg agaauacacg aaacaccuca ucagagucga aaacuggaua      180

uuuaggaacc ccggcuucgc ccuagcagca gccgcaaucg cguggcugcu gggcagcuca      240

acaucgcaaa aagugauaua ccuaguaaug auccuccuaa uagccccggc auacagcaua      300

cgcugcaucg gaguaucaaa ccgagacuuu gucgagggua uguccggcgg aacaugggua      360

gacgugguuc ucgaacacgg cggcugcgua accguaaugg cacaagacaa accaaccgug      420

gacaucgaac ucgucacaac aaccguaagc aacauggccg agguacgauc cuacugcuac      480

gaagccagca uuucagacau ggcaagcgac uccagaugcc caacccaggg cgaagccuac      540

cucgacaaac aaagcgacac acaauacgua ugcaaacgaa cacucgugga ccgaggcugg      600

ggaaacggcu gcggacuguu cggaaaagga agccuaguga ccugcgcgaa auucgccugc      660

uccaagaaaa ugacaggaaa aucaauccag ccugaaaacc ucgaguaccg aauaaugcuc      720

agcguccaug gaagccagca cuccggcaug auaguaaacg acacaggaca cgaaacggac      780

gaaaacagag caaaaguaga aaucacccca aacucaccca gagccgaagc aacacuugga      840

ggcuucggcu cacucggacu agacugcgag ccacgcaccg gacucgacuu cagcgaccuc      900

uacuaccuaa ccaugaacaa caaacacugg cuuguacaca aagaaugguu ccacgacaua      960

ccccucccuu ggcacgcagg agcagacacc ggaacacccc acuggaacaa caaagaagca     1020

cugguagagu ucaaagacgc acacgcaaaa cgacaaaccg uagucgugcu gggcagucaa     1080

gaaggcgccg ugcacaccgc ccucgcugga gcccuugaag cagaaaugga cggggccaaa     1140

ggcagacucu cauccggcca ccucaaaugc cgacucaaaa uggacaaacu uagacuaaaa     1200

ggcguaagcu acagccugug cacggcggca uucacauuca cgaaaauacc agccgagacc     1260

cuacacggca cugucaccgu agaaguacag uacgcaggca cagacgggcc cugcaaagua     1320

cccgcacaaa uggccguuga caugcaaacc cuaaccccag ucggccgccu caucaccgcc     1380

aaccccguaa uaacagaaag cacagaaaac agcaaaauga ugcucgaacu cgacccaccg     1440

uucggagaca gcuacaucgu aaucggcgug ggcgaaaaga aaauaacgca ucacuggcac     1500

cgaucaggau caaccaucgg caaagccuuc gaagcaaccg uucgcggcgc aaaaagaaug     1560

gccguacuag gcgacacggc augggauuuc gguagcguag ggggcgcccu aaacucccua     1620

ggcaaaggca uccaccaaau cuucggcgcc gcauucaaau cacuauucgg cggaaugagc     1680

ugguucagcc aaauccucau cggcacccuc cucauguggu uaggacugaa ugccaaaaac     1740

ggaagcaucu cacucaugug ccuagcccug ggaggaguau uaaucuuccu aucuacagca     1800

guaucggcc                                                             1809


<210>  9999
<211>  1797
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant CDS opt

<400>  9999
augggcaaaa gaucagccgg aucaaucaug uggcuugcaa gccucgcugu aguaaucgcc       60

ugcgcuggag cagcagucac ccuccccuca cacuccacca gaaaacuaca aacacgcucc      120

caaacauggc ucgaaucacg agaauacacc aaacaccuca ucaaggucga aaacuggauc      180

uuccgaaacc ccggauucgc acucguagca guagccaucg cauggcuacu cggauccuca      240

accagccaga aaguaauaua ccuagucaug auccuccuga uagcacccgc cuacagcauc      300

cgaugcaucg gcgucagcaa ccgagacuuc gucgaaggca ugucaggggg aaccuggguc      360

gacgucgucc uagagcacgg cggcugcgua acagucaugg cacaagacaa gcccaccguc      420

gacauagaac uggucacaac uaccguaucc aacauggccg agguaagauc cuacugcuac      480

gaagccagca uaagcgacau ggccagcgac aguaggugcc cuacacaagg cgaagccuac      540

cucgauaaac aaucagacac ccaauauguc uguaaacgca cucuaguaga cagaggaugg      600

ggcaacggcu gcggccuauu uggcaaaggc agccuaguca caugcgcaaa guuuaccugc      660

agcaagaaaa ugaccggaaa aucaauccaa ccagaaaacc ucgaauaccg aaucaugcuc      720

ucaguacacg gaucgcaaca cucaggcaug aucggcuaug aaaccgacga agaccgugcc      780

aaaguagaag uaaccccaaa cagcccacga gcugaagcaa cccuaggagg cuucggaucc      840

cucgggcucg acugcgaacc aagaaccggc cuagacuucu ccgaccugua cuaccucacg      900

augaacaaca aacacuggcu cguacacaag gaaugguucc acgacauacc gcuacccugg      960

cacgcaggcg cagacacagg cacgccacac uggaacaaca aagaagcccu aguggaguuc     1020

aaagacgcac acgcaaaacg ccagaccgug gucguccuag ggagccaaga aggagccgua     1080

cacacagccc uggccggcgc ccuugaagcc gaaauggacg gcgcaaaagg ccgacucuuc     1140

agcggacacc ucaaaugccg ucucaaaaug gacaaacuac ggcucaaagg cguauccuau     1200

ucacucugca ccgccgccuu cacauucaca aaggucccug cggaaacucu acacggaaca     1260

gucacagucg aagugcaaua cgcaggaacc gacggcccuu gcaagauacc aguccagaug     1320

gccgucgaca ugcaaacccu cacgccaguc ggccgacuca ucaccgcaaa ccccguaauc     1380

acagaaucaa cggaaaacuc aaagaugaug cucgagcucg acccucccuu cggagacuca     1440

uacauaguca ucggggucgg cgacaagaaa auaacccacc acuggcacag aagcggcucc     1500

accaucggaa aagccuucga agcgacaguc cgaggcgcaa aacgcauggc cguacucgga     1560

gacaccgcgu gggacuuugg cucaguaggc ggcguguuua acagccucgg aaaaggaauc     1620

caccaaauau ucggagcagc guucaaauca cuguucggcg ggauguccug guucucccaa     1680

auccucaucg gaacacuucu cguauggcua ggccuaaaca ccaaaaacgg aucaaucuca     1740

cuaacaugcc ucgcccucgg aggagucaug auauuccugu cgacugcggu cucggcc        1797


<210>  10000
<211>  1809
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant CDS opt

<400>  10000
augggaaagc gauccgcagg auccauaaug uggcucgccu cccuagccgu cguaauagcg       60

ugcgccggcg cagccguaac ccuacccucc cacagcaccc gcaagcuaca aacacgcuca      120

caaaccuggc uugaaucacg cgaguacacu aaacaccuaa uacgcguaga aaacuggauc      180

uuccguaacc ccggguucgc acucgccgca gccgccaucg ccuggcuucu aggaagcucc      240

acaucacaaa aagucaucua ccuagucaug auccuccuca uugccccagc cuacaguauc      300

agaugcaucg gugucuccaa ccgggacuuc gucgaaggga uguccggagg aacaugggua      360

gacgucgucc ucgaacacgg cggcugcgua accguaaugg cgcaggacaa accgacuguc      420

gacauagagc uaguaaccac uaccgucucc aacauggcag aaguacgauc cuacugcuac      480

gaagccagca uuagcgacau ggcauccgac agccgcugcc ccacccaagg cgaagccuac      540

cucgacaaac aaucagacac ccaauacguc ugcaaacgca cacucgucga ccguggcugg      600

ggcaacggcu gcggacucuu cggaaagggc ucccuaguua ccugcgcaaa guucgccugu      660

uccaagaaaa ugaccggcaa aucgauacaa ccagaaaacc ucgaauaucg aauaaugcuc      720

uccguccacg gcagccaaca cagcggcaug auaguaaacg acaccggaca cgaaacagac      780

gaaaaccgcg cuaaagucga aauaacaccc aacucacccc gggccgaagc cacccucggc      840

ggcuucggau cacucggccu cgacugcgag cccagaaccg gccuagacuu cucggaccuc      900

uacuaucuaa caaugaacaa caaacacugg cucguccaca aagaaugguu ccacgacauc      960

ccacuccccu ggcacgccgg agccgacacc ggaacaccuc acuggaacaa caaagaagcc     1020

cucgucgagu ucaaagacgc ccacgccaaa cgccagacag ugguaguacu aggcagccaa     1080

gaaggagccg uccacacggc ccuagcagga gcccuggaag ccgaaaugga cggagcaaaa     1140

ggcagacucu cuagcggcca ccucaagugc cgccuaaaaa uggacaaacu acgacuaaaa     1200

ggcguuuccu acagccugug caccgccgcc uucacguuca cuaaaauacc cgccgaaacc     1260

cuccacggca ccguaacagu cgaaguccaa uacgcaggaa ccgacggacc gugcaaagua     1320

ccugcacaaa uggccguaga caugcagacc cugaccccag uaggcagacu aaucacagcc     1380

aaccccguaa uaacggaauc aaccgaaaac ucaaaaauga ugcuugaacu agacccgccc     1440

uucggggaca guuacaucgu aaucggcguc ggagaaaaga aaauaaccca ccacuggcac     1500

agauccggau caaccaucgg aaaggccuuc gaagcaaccg uccggggagc caaacgcaug     1560

gccguccuag gagacacagc cugggacuuc ggcagugucg gcggcgcccu caacagucug     1620

ggaaaaggca uacaccaaau cuucggcgca gcauucaaga gccuauucgg gggaaugagc     1680

ugguucuccc aaauacuaau aggaacccuc cuaauguggc ugggacucaa caccaaaaac     1740

ggcagcaucu cacucaugug ccucgcccuc ggcggaguac ugauauuccu cucuacagca     1800

gucuccgcc                                                             1809


<210>  10001
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 wild 
       type

<400>  10001
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10002
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 wild 
       type

<400>  10002
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacacgugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauguuguc      660

uuggaacaug gagguugugu cacuguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagccuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaaaguug agauaacgcc caauucacca agagccgaag ccacccuggg gggguuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug gcugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guacugcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa ucuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acgcaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10003
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 wild type

<400>  10003
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu aucacuagac gcgggagugc auacuacaug      120

uacuuggaua ggagcgaugc cgggaaggcc auuucguuug cuaccacauu gggagugaac      180

aagugccacg uacagaucau ggaccucggg cacaugugug acgccaccau gaguuaugag      240

ugcccuaugc uggaugaggg aguggaacca gaugaugucg auugcuggug caacacgaca      300

ucaacuuggg uuguguacgg aaccugucau cacaaaaaag gugaggcacg gcgaucuaga      360

agagccguga cgcucccuuc ucacucuaca aggaaguugc aaacgcgguc gcagaccugg      420

uuagaaucaa gagaauacac gaagcacuug aucaagguug aaaacuggau auucaggaac      480

cccggguuug cgcuaguggc cguugccauu gccuggcuuu ugggaagcuc gacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacaguau caggugcauu      600

ggagucagca auagagacuu cguggagggc augucaggug ggaccugggu ugauguuguc      660

uuggaacaug gaggcugcgu uaccgugaug gcacaggaca agccaacagu cgacauagag      720

uuggucacga cgacgguuag uaacauggcc gagguaagau ccuauugcua cgaggcaucg      780

auaucggaca uggcuucgga cagucguugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acauuagugg acagagguug gggaaacggu      900

uguggacuuu uuggcaaagg gagcuuggug acaugugcca aguuuacgug uucuaagaag      960

augaccggga agagcauuca accggaaaau cuggaguauc ggauaaugcu aucagugcau     1020

ggcucccagc auagcgggau gauuggauau gaaacugacg aagauagagc gaaagucgag     1080

guuacgccua auucaccaag agcggaagca accuugggag gcuuuggaag uuuaggacuu     1140

gacugugaac caaggacagg ccuugacuuu ucagaucugu auuaccugac caugaacaau     1200

aagcauuggu uggugcacaa agagugguuu caugacaucc cauugccuug gcaugcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaggcau ugguagaauu uaaggaugcc     1320

cacgccaaga ggcaaaccgu cgucguucug gggagccagg aaggagccgu ucacacggcu     1380

cucgcuggag cucuagaggc ugagauggau ggugcaaagg gaaggcuguu cucuggccau     1440

uugaaaugcc gccuaaaaau ggacaagcuc agauugaagg gcgugucaua uuccuugugc     1500

acugcggcau ucacauucac caagguccca gcugaaacac ugcauggaac agucacagug     1560

gaggugcagu augcagggac agauggaccc ugcaagaucc caguccagau ggcgguggac     1620

augcagaccc ugaccccagu uggaaggcug auaaccgcca accccgugau uacugaaagc     1680

acugagaacu caaagaugau guuggagcuu gacccaccau uuggggauuc uuacauuguc     1740

auaggaguug gggacaagaa aaucacccac cacuggcaua ggagugguag caccaucgga     1800

aaggcauuug aggccacugu gagaggcgcc aagagaaugg caguccuggg ggauacagcc     1860

ugggacuucg gaucagucgg ggguguguuc aacucacugg guaagggcau ucaccagauu     1920

uuuggagcag ccuucaaauc acuguuugga ggaauguccu gguucucaca gauccucaua     1980

ggcacgcugc uagugugguu agguuugaac acaaagaaug gaucuaucuc ccucacaugc     2040

uuggcccugg ggggagugau gaucuuccuc uccacggcug uuucugcuug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10004
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 wild type

<400>  10004
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauguuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagccuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguccac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauccuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10005
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 wild 
       type

<400>  10005
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10006
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 wild 
       type

<400>  10006
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10007
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 wild type

<400>  10007
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagca ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10008
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 wild type

<400>  10008
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10009
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 wild 
       type

<400>  10009
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10010
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 wild 
       type

<400>  10010
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac acgugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uguugucuug      660

gaacauggag guugugucac uguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aaaguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg guuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacuggcu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua cugcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaaucu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaacg caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10011
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 wild type

<400>  10011
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccauc acuagacgcg ggagugcaua cuacauguac      120

uuggauagga gcgaugccgg gaaggccauu ucguuugcua ccacauuggg agugaacaag      180

ugccacguac agaucaugga ccucgggcac augugugacg ccaccaugag uuaugagugc      240

ccuaugcugg augagggagu ggaaccagau gaugucgauu gcuggugcaa cacgacauca      300

acuuggguug uguacggaac cugucaucac aaaaaaggug aggcacggcg aucuagaaga      360

gccgugacgc ucccuucuca cucuacaagg aaguugcaaa cgcggucgca gaccugguua      420

gaaucaagag aauacacgaa gcacuugauc aagguugaaa acuggauauu caggaacccc      480

ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg gaagcucgac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acaguaucag gugcauugga      600

gucagcaaua gagacuucgu ggagggcaug ucagguggga ccuggguuga uguugucuug      660

gaacauggag gcugcguuac cgugauggca caggacaagc caacagucga cauagaguug      720

gucacgacga cgguuaguaa cauggccgag guaagauccu auugcuacga ggcaucgaua      780

ucggacaugg cuucggacag ucguugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaaca uuaguggaca gagguugggg aaacgguugu      900

ggacuuuuug gcaaagggag cuuggugaca ugugccaagu uuacguguuc uaagaagaug      960

accgggaaga gcauucaacc ggaaaaucug gaguaucgga uaaugcuauc agugcauggc     1020

ucccagcaua gcgggaugau uggauaugaa acugacgaag auagagcgaa agucgagguu     1080

acgccuaauu caccaagagc ggaagcaacc uugggaggcu uuggaaguuu aggacuugac     1140

ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu accugaccau gaacaauaag     1200

cauugguugg ugcacaaaga gugguuucau gacaucccau ugccuuggca ugcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaggcauugg uagaauuuaa ggaugcccac     1320

gccaagaggc aaaccgucgu cguucugggg agccaggaag gagccguuca cacggcucuc     1380

gcuggagcuc uagaggcuga gauggauggu gcaaagggaa ggcuguucuc uggccauuug     1440

aaaugccgcc uaaaaaugga caagcucaga uugaagggcg ugucauauuc cuugugcacu     1500

gcggcauuca cauucaccaa ggucccagcu gaaacacugc auggaacagu cacaguggag     1560

gugcaguaug cagggacaga uggacccugc aagaucccag uccagauggc gguggacaug     1620

cagacccuga ccccaguugg aaggcugaua accgccaacc ccgugauuac ugaaagcacu     1680

gagaacucaa agaugauguu ggagcuugac ccaccauuug gggauucuua cauugucaua     1740

ggaguugggg acaagaaaau cacccaccac uggcauagga gugguagcac caucggaaag     1800

gcauuugagg ccacugugag aggcgccaag agaauggcag uccuggggga uacagccugg     1860

gacuucggau cagucggggg uguguucaac ucacugggua agggcauuca ccagauuuuu     1920

ggagcagccu ucaaaucacu guuuggagga auguccuggu ucucacagau ccucauaggc     1980

acgcugcuag ugugguuagg uuugaacaca aagaauggau cuaucucccu cacaugcuug     2040

gcccuggggg gagugaugau cuuccucucc acggcuguuu cugcuugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10012
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 wild type

<400>  10012
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uguugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguccacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auccucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10013
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 wild 
       type

<400>  10013
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcacgcugg gcaaggccuu uucaacaacu uugaagggag cucaaagacu ggcagcguug     1860

ggcgacacag ccugggacuu uggcucuauu ggaggggucu ucaacuccau aggaagagcc     1920

guucaccaag uguuuggugg ugccuucaga acacucuuug ggggaauguc uuggaucaca     1980

caagggcuaa ugggugcccu acugcucugg augggcguca acgcacgaga ccgaucaauu     2040

gcuuuggccu ucuuagccac aggaggugug cucguguucu uagcgaccaa ugugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10014
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 wild 
       type

<400>  10014
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacacgugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauguuguc      660

uuggaacaug gagguugugu cacuguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagccuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaaaguug agauaacgcc caauucacca agagccgaag ccacccuggg gggguuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug gcugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guacugcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcacgcugg gcaaggccuu uucaacaacu uugaagggag cucaaagacu ggcagcguug     1860

ggcgacacag ccugggacuu uggcucuauu ggaggggucu ucaacuccau aggaagagcc     1920

guucaccaag uguuuggugg ugccuucaga acacucuuug ggggaauguc uuggaucaca     1980

caagggcuaa ugggugcccu acugcucugg augggcguca acgcacgaga ccgaucaauu     2040

gcuuuggccu ucuuagccac aggaggugug cucguguucu uagcgaccaa ugugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10015
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 wild type

<400>  10015
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu aucacuagac gcgggagugc auacuacaug      120

uacuuggaua ggagcgaugc cgggaaggcc auuucguuug cuaccacauu gggagugaac      180

aagugccacg uacagaucau ggaccucggg cacaugugug acgccaccau gaguuaugag      240

ugcccuaugc uggaugaggg aguggaacca gaugaugucg auugcuggug caacacgaca      300

ucaacuuggg uuguguacgg aaccugucau cacaaaaaag gugaggcacg gcgaucuaga      360

agagccguga cgcucccuuc ucacucuaca aggaaguugc aaacgcgguc gcagaccugg      420

uuagaaucaa gagaauacac gaagcacuug aucaagguug aaaacuggau auucaggaac      480

cccggguuug cgcuaguggc cguugccauu gccuggcuuu ugggaagcuc gacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacaguau caggugcauu      600

ggagucagca auagagacuu cguggagggc augucaggug ggaccugggu ugauguuguc      660

uuggaacaug gaggcugcgu uaccgugaug gcacaggaca agccaacagu cgacauagag      720

uuggucacga cgacgguuag uaacauggcc gagguaagau ccuauugcua cgaggcaucg      780

auaucggaca uggcuucgga cagucguugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acauuagugg acagagguug gggaaacggu      900

uguggacuuu uuggcaaagg gagcuuggug acaugugcca aguuuacgug uucuaagaag      960

augaccggga agagcauuca accggaaaau cuggaguauc ggauaaugcu aucagugcau     1020

ggcucccagc auagcgggau gauuggauau gaaacugacg aagauagagc gaaagucgag     1080

guuacgccua auucaccaag agcggaagca accuugggag gcuuuggaag uuuaggacuu     1140

gacugugaac caaggacagg ccuugacuuu ucagaucugu auuaccugac caugaacaau     1200

aagcauuggu uggugcacaa agagugguuu caugacaucc cauugccuug gcaugcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaggcau ugguagaauu uaaggaugcc     1320

cacgccaaga ggcaaaccgu cgucguucug gggagccagg aaggagccgu ucacacggcu     1380

cucgcuggag cucuagaggc ugagauggau ggugcaaagg gaaggcuguu cucuggccau     1440

uugaaaugcc gccuaaaaau ggacaagcuc agauugaagg gcgugucaua uuccuugugc     1500

acugcggcau ucacauucac caagguccca gcugaaacac ugcauggaac agucacagug     1560

gaggugcagu augcagggac agauggaccc ugcaagaucc caguccagau ggcgguggac     1620

augcagaccc ugaccccagu uggaaggcug auaaccgcca accccgugau uacugaaagc     1680

acugagaacu caaagaugau guuggagcuu gacccaccau uuggggauuc uuacauuguc     1740

auaggaguug gggacaagaa aaucacccac cacuggcaua ggaguggcag cacgcugggc     1800

aaggccuuuu caacaacuuu gaagggagcu caaagacugg cagcguuggg cgacacagcc     1860

ugggacuuug gcucuauugg aggggucuuc aacuccauag gaagagccgu ucaccaagug     1920

uuugguggug ccuucagaac acucuuuggg ggaaugucuu ggaucacaca agggcuaaug     1980

ggugcccuac ugcucuggau gggcgucaac gcacgagacc gaucaauugc uuuggccuuc     2040

uuagccacag gaggugugcu cguguucuua gcgaccaaug ugcacgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10016
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 wild type

<400>  10016
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauguuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagccuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguccac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcacgcugg gcaaggccuu uucaacaacu uugaagggag cucaaagacu ggcagcguug     1860

ggcgacacag ccugggacuu uggcucuauu ggaggggucu ucaacuccau aggaagagcc     1920

guucaccaag uguuuggugg ugccuucaga acacucuuug ggggaauguc uuggaucaca     1980

caagggcuaa ugggugcccu acugcucugg augggcguca acgcacgaga ccgaucaauu     2040

gcuuuggccu ucuuagccac aggaggugug cucguguucu uagcgaccaa ugugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10017
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 wild 
       type

<400>  10017
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10018
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 wild 
       type

<400>  10018
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10019
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 wild type

<400>  10019
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagca ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gaguggcagc acgcugggca aggccuuuuc aacaacuuug     1800

aagggagcuc aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga     1860

ggggucuuca acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca     1920

cucuuugggg gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug     1980

ggcgucaacg cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc     2040

guguucuuag cgaccaaugu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10020
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 wild type

<400>  10020
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcacgcuggg caaggccuuu     1800

ucaacaacuu ugaagggagc ucaaagacug gcagcguugg gcgacacagc cugggacuuu     1860

ggcucuauug gaggggucuu caacuccaua ggaagagccg uucaccaagu guuugguggu     1920

gccuucagaa cacucuuugg gggaaugucu uggaucacac aagggcuaau gggugcccua     1980

cugcucugga ugggcgucaa cgcacgagac cgaucaauug cuuuggccuu cuuagccaca     2040

ggaggugugc ucguguucuu agcgaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10021
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 wild 
       type

<400>  10021
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

acgcugggca aggccuuuuc aacaacuuug aagggagcuc aaagacuggc agcguugggc     1860

gacacagccu gggacuuugg cucuauugga ggggucuuca acuccauagg aagagccguu     1920

caccaagugu uugguggugc cuucagaaca cucuuugggg gaaugucuug gaucacacaa     1980

gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg cacgagaccg aucaauugcu     2040

uuggccuucu uagccacagg aggugugcuc guguucuuag cgaccaaugu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10022
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 wild 
       type

<400>  10022
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac acgugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uguugucuug      660

gaacauggag guugugucac uguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aaaguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg guuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacuggcu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua cugcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

acgcugggca aggccuuuuc aacaacuuug aagggagcuc aaagacuggc agcguugggc     1860

gacacagccu gggacuuugg cucuauugga ggggucuuca acuccauagg aagagccguu     1920

caccaagugu uugguggugc cuucagaaca cucuuugggg gaaugucuug gaucacacaa     1980

gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg cacgagaccg aucaauugcu     2040

uuggccuucu uagccacagg aggugugcuc guguucuuag cgaccaaugu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10023
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 wild type

<400>  10023
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccauc acuagacgcg ggagugcaua cuacauguac      120

uuggauagga gcgaugccgg gaaggccauu ucguuugcua ccacauuggg agugaacaag      180

ugccacguac agaucaugga ccucgggcac augugugacg ccaccaugag uuaugagugc      240

ccuaugcugg augagggagu ggaaccagau gaugucgauu gcuggugcaa cacgacauca      300

acuuggguug uguacggaac cugucaucac aaaaaaggug aggcacggcg aucuagaaga      360

gccgugacgc ucccuucuca cucuacaagg aaguugcaaa cgcggucgca gaccugguua      420

gaaucaagag aauacacgaa gcacuugauc aagguugaaa acuggauauu caggaacccc      480

ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg gaagcucgac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acaguaucag gugcauugga      600

gucagcaaua gagacuucgu ggagggcaug ucagguggga ccuggguuga uguugucuug      660

gaacauggag gcugcguuac cgugauggca caggacaagc caacagucga cauagaguug      720

gucacgacga cgguuaguaa cauggccgag guaagauccu auugcuacga ggcaucgaua      780

ucggacaugg cuucggacag ucguugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaaca uuaguggaca gagguugggg aaacgguugu      900

ggacuuuuug gcaaagggag cuuggugaca ugugccaagu uuacguguuc uaagaagaug      960

accgggaaga gcauucaacc ggaaaaucug gaguaucgga uaaugcuauc agugcauggc     1020

ucccagcaua gcgggaugau uggauaugaa acugacgaag auagagcgaa agucgagguu     1080

acgccuaauu caccaagagc ggaagcaacc uugggaggcu uuggaaguuu aggacuugac     1140

ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu accugaccau gaacaauaag     1200

cauugguugg ugcacaaaga gugguuucau gacaucccau ugccuuggca ugcuggggca     1260

gacaccggaa cuccacacug gaacaacaaa gaggcauugg uagaauuuaa ggaugcccac     1320

gccaagaggc aaaccgucgu cguucugggg agccaggaag gagccguuca cacggcucuc     1380

gcuggagcuc uagaggcuga gauggauggu gcaaagggaa ggcuguucuc uggccauuug     1440

aaaugccgcc uaaaaaugga caagcucaga uugaagggcg ugucauauuc cuugugcacu     1500

gcggcauuca cauucaccaa ggucccagcu gaaacacugc auggaacagu cacaguggag     1560

gugcaguaug cagggacaga uggacccugc aagaucccag uccagauggc gguggacaug     1620

cagacccuga ccccaguugg aaggcugaua accgccaacc ccgugauuac ugaaagcacu     1680

gagaacucaa agaugauguu ggagcuugac ccaccauuug gggauucuua cauugucaua     1740

ggaguugggg acaagaaaau cacccaccac uggcauagga guggcagcac gcugggcaag     1800

gccuuuucaa caacuuugaa gggagcucaa agacuggcag cguugggcga cacagccugg     1860

gacuuuggcu cuauuggagg ggucuucaac uccauaggaa gagccguuca ccaaguguuu     1920

gguggugccu ucagaacacu cuuuggggga augucuugga ucacacaagg gcuaaugggu     1980

gcccuacugc ucuggauggg cgucaacgca cgagaccgau caauugcuuu ggccuucuua     2040

gccacaggag gugugcucgu guucuuagcg accaaugugc acgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10024
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 wild type

<400>  10024
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uguugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguccacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

acgcugggca aggccuuuuc aacaacuuug aagggagcuc aaagacuggc agcguugggc     1860

gacacagccu gggacuuugg cucuauugga ggggucuuca acuccauagg aagagccguu     1920

caccaagugu uugguggugc cuucagaaca cucuuugggg gaaugucuug gaucacacaa     1980

gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg cacgagaccg aucaauugcu     2040

uuggccuucu uagccacagg aggugugcuc guguucuuag cgaccaaugu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10025
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 wild type

<400>  10025
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      120

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      180

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      240

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      300

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      360

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      420

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      480

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      540

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      600

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      660

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau      720

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga      780

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga      840

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug      900

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu      960

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1020

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1080

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1140

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1200

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1260

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1320

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1380

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1440

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1500

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1560

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1620

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1680

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     1740

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10026
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 wild type

<400>  10026
gggagaaagc uuaccaugac gagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      120

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      180

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      240

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      300

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauguuguc      360

uuggaacaug gagguugugu cacuguaaug gcacaggaca aaccgacugu cgacauagag      420

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      480

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      540

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      600

uguggacuuu uuggcaaagg gagccuggug acaugcgcua aguuugcaug cuccaagaaa      660

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau      720

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga      780

gcgaaaguug agauaacgcc caauucacca agagccgaag ccacccuggg gggguuugga      840

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug      900

acuaugaaua acaagcacug gcugguucac aaggaguggu uccacgacau uccauuaccu      960

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1020

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1080

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1140

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1200

uacuccuugu guacugcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1260

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1320

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1380

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1440

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1500

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1560

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1620

auccaucaaa ucuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1680

caaauucuca uuggaacguu gcugaugugg uugggucuga acgcaaagaa uggaucuauu     1740

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10027
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 wild type

<400>  10027
gggagaaagc uuaccaugac cagcaucgga aucauuggcc uccugcugac uacagccaug       60

gcagccguga cgcucccuuc ucacucuaca aggaaguugc aaacgcgguc gcagaccugg      120

uuagaaucaa gagaauacac gaagcacuug aucaagguug aaaacuggau auucaggaac      180

cccggguuug cgcuaguggc cguugccauu gccuggcuuu ugggaagcuc gacgagccaa      240

aaagucauau acuuggucau gauacugcug auugccccgg cauacaguau caggugcauu      300

ggagucagca auagagacuu cguggagggc augucaggug ggaccugggu ugauguuguc      360

uuggaacaug gaggcugcgu uaccgugaug gcacaggaca agccaacagu cgacauagag      420

uuggucacga cgacgguuag uaacauggcc gagguaagau ccuauugcua cgaggcaucg      480

auaucggaca uggcuucgga cagucguugc ccaacacaag gugaagccua ccuugacaag      540

caaucagaca cucaauaugu cugcaaaaga acauuagugg acagagguug gggaaacggu      600

uguggacuuu uuggcaaagg gagcuuggug acaugugcca aguuuacgug uucuaagaag      660

augaccggga agagcauuca accggaaaau cuggaguauc ggauaaugcu aucagugcau      720

ggcucccagc auagcgggau gauuggauau gaaacugacg aagauagagc gaaagucgag      780

guuacgccua auucaccaag agcggaagca accuugggag gcuuuggaag uuuaggacuu      840

gacugugaac caaggacagg ccuugacuuu ucagaucugu auuaccugac caugaacaau      900

aagcauuggu uggugcacaa agagugguuu caugacaucc cauugccuug gcaugcuggg      960

gcagacaccg gaacuccaca cuggaacaac aaagaggcau ugguagaauu uaaggaugcc     1020

cacgccaaga ggcaaaccgu cgucguucug gggagccagg aaggagccgu ucacacggcu     1080

cucgcuggag cucuagaggc ugagauggau ggugcaaagg gaaggcuguu cucuggccau     1140

uugaaaugcc gccuaaaaau ggacaagcuc agauugaagg gcgugucaua uuccuugugc     1200

acugcggcau ucacauucac caagguccca gcugaaacac ugcauggaac agucacagug     1260

gaggugcagu augcagggac agauggaccc ugcaagaucc caguccagau ggcgguggac     1320

augcagaccc ugaccccagu uggaaggcug auaaccgcca accccgugau uacugaaagc     1380

acugagaacu caaagaugau guuggagcuu gacccaccau uuggggauuc uuacauuguc     1440

auaggaguug gggacaagaa aaucacccac cacuggcaua ggagugguag caccaucgga     1500

aaggcauuug aggccacugu gagaggcgcc aagagaaugg caguccuggg ggauacagcc     1560

ugggacuucg gaucagucgg ggguguguuc aacucacugg guaagggcau ucaccagauu     1620

uuuggagcag ccuucaaauc acuguuugga ggaauguccu gguucucaca gauccucaua     1680

ggcacgcugc uagugugguu agguuugaac acaaagaaug gaucuaucuc ccucacaugc     1740

uuggcccugg ggggagugau gaucuuccuc uccacggcug uuucugcuug aggacuaguu     1800

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     1860

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  10028
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 wild type

<400>  10028
gggagaaagc uuaccaugac cagugucgga auuguuggcc uccugcugac cacagcuaug       60

gcagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      120

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      180

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      240

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      300

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauguuguc      360

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      420

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      480

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      540

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      600

uguggacuuu uuggcaaagg gagccuggug acaugcgcua aguuugcaug cuccaagaaa      660

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau      720

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga      780

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga      840

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug      900

acuaugaaua acaagcacug guugguccac aaggaguggu uccacgacau uccauuaccu      960

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1020

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1080

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1140

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1200

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1260

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1320

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1380

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1440

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1500

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1560

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1620

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1680

caaauccuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     1740

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10029
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 wild type

<400>  10029
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gcugugacgc uccccuccca uuccacuagg      120

aagcugcaaa cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu      180

agagucgaaa auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu      240

uggcuuuugg gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu      300

gccccggcau acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug      360

ucagguggga cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca      420

caggacaaac cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag      480

guaagauccu acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca      540

acacaaggug aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg      600

uuaguggaca gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca      660

ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug      720

gaguaccgga uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac      780

acaggacaug aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga      840

gccgaagcca cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc      900

cuugacuuuu cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag      960

gagugguucc acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac     1020

uggaacaaca aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc     1080

gugguucuag ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu     1140

gagauggaug gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug     1200

gauaaacuua gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc     1260

aagaucccgg cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca     1320

gauggaccuu gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu     1380

gggagguuga uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug     1440

cuggaacuug auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag     1500

aucacccacc acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug     1560

agaggugcca agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga     1620

ggcgcucuca acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca     1680

uuguuuggag gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug     1740

ggucugaaca caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug     1800

aucuucuuau ccacagccgu cucugcuuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10030
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 wild type

<400>  10030
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gcugugacgc uccccuccca uuccacuagg      120

aagcugcaaa cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu      180

agagucgaaa auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu      240

uggcuuuugg gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu      300

gccccggcau acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug      360

ucagguggga cuuggguuga uguugucuug gaacauggag guugugucac uguaauggca      420

caggacaaac cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag      480

guaagauccu acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca      540

acacaaggug aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg      600

uuaguggaca gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca      660

ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug      720

gaguaccgga uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac      780

acaggacaug aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga      840

gccgaagcca cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc      900

cuugacuuuu cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag      960

gagugguucc acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac     1020

uggaacaaca aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc     1080

gugguucuag ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu     1140

gagauggaug gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug     1200

gauaaacuua gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc     1260

aagaucccgg cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca     1320

gauggaccuu gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu     1380

gggagguuga uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug     1440

cuggaacuug auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag     1500

aucacccacc acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug     1560

agaggugcca agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga     1620

ggcgcucuca acucauuggg caagggcauc caucaaaucu uuggagcagc uuucaaauca     1680

uuguuuggag gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug     1740

ggucugaacg caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug     1800

aucuucuuau ccacagccgu cucugcuuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10031
<211>  2020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 wild type

<400>  10031
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gccgugacgc ucccuucuca cucuacaagg      120

aaguugcaaa cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc      180

aagguugaaa acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc      240

uggcuuuugg gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu      300

gccccggcau acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug      360

ucagguggga ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca      420

caggacaagc caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag      480

guaagauccu auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca      540

acacaaggug aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca      600

uuaguggaca gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca      660

ugugccaagu uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug      720

gaguaucgga uaaugcuauc agugcauggc ucccagcaua gcgggaugau uggauaugaa      780

acugacgaag auagagcgaa agucgagguu acgccuaauu caccaagagc ggaagcaacc      840

uugggaggcu uuggaaguuu aggacuugac ugugaaccaa ggacaggccu ugacuuuuca      900

gaucuguauu accugaccau gaacaauaag cauugguugg ugcacaaaga gugguuucau      960

gacaucccau ugccuuggca ugcuggggca gacaccggaa cuccacacug gaacaacaaa     1020

gaggcauugg uagaauuuaa ggaugcccac gccaagaggc aaaccgucgu cguucugggg     1080

agccaggaag gagccguuca cacggcucuc gcuggagcuc uagaggcuga gauggauggu     1140

gcaaagggaa ggcuguucuc uggccauuug aaaugccgcc uaaaaaugga caagcucaga     1200

uugaagggcg ugucauauuc cuugugcacu gcggcauuca cauucaccaa ggucccagcu     1260

gaaacacugc auggaacagu cacaguggag gugcaguaug cagggacaga uggacccugc     1320

aagaucccag uccagauggc gguggacaug cagacccuga ccccaguugg aaggcugaua     1380

accgccaacc ccgugauuac ugaaagcacu gagaacucaa agaugauguu ggagcuugac     1440

ccaccauuug gggauucuua cauugucaua ggaguugggg acaagaaaau cacccaccac     1500

uggcauagga gugguagcac caucggaaag gcauuugagg ccacugugag aggcgccaag     1560

agaauggcag uccuggggga uacagccugg gacuucggau cagucggggg uguguucaac     1620

ucacugggua agggcauuca ccagauuuuu ggagcagccu ucaaaucacu guuuggagga     1680

auguccuggu ucucacagau ccucauaggc acgcugcuag ugugguuagg uuugaacaca     1740

aagaauggau cuaucucccu cacaugcuug gcccuggggg gagugaugau cuuccucucc     1800

acggcuguuu cugcuugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     1860

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1980

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2020


<210>  10032
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 wild type

<400>  10032
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gcugugacgc uccccuccca uuccacuagg      120

aagcugcaaa cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu      180

agagucgaaa auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu      240

uggcuuuugg gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu      300

gccccggcau acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug      360

ucagguggga cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca      420

caggacaaac cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag      480

guaagauccu acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca      540

acacaaggug aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg      600

uuaguggaca gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca      660

ugcgcuaagu uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug      720

gaguaccgga uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac      780

acaggacaug aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga      840

gccgaagcca cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc      900

cuugacuuuu cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag      960

gagugguucc acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac     1020

uggaacaaca aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc     1080

gugguucuag ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu     1140

gagauggaug gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug     1200

gauaaacuua gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc     1260

aagaucccgg cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca     1320

gauggaccuu gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu     1380

gggagguuga uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug     1440

cuggaacuug auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag     1500

aucacccacc acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug     1560

agaggugcca agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga     1620

ggcgcucuca acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca     1680

uuguuuggag gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug     1740

ggucugaaca caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug     1800

aucuucuuau ccacagccgu cucugcuuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10033
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 wild type

<400>  10033
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      120

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      180

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      240

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      300

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      360

auugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      420

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      480

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      540

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      600

aauggaugug gacuuuuugg caaagggagu cuggugacau gcgcuaaguu ugcaugcucc      660

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      720

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      780

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      840

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      900

uacuugacua ugaauaacaa gcacugguug guucacaagg agugguucca cgacauucca      960

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1020

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1080

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1140

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1200

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1260

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1320

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1380

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1440

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1500

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1560

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1620

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1680

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacac aaagaaugga     1740

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     1800

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10034
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 wild type

<400>  10034
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      120

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      180

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      240

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      300

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      360

guugucuugg aacauggagg uugugucacu guaauggcac aggacaaacc gacugucgac      420

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      480

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      540

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      600

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      660

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      720

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      780

aauagagcga aaguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggg      840

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      900

uacuugacua ugaauaacaa gcacuggcug guucacaagg agugguucca cgacauucca      960

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1020

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1080

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1140

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1200

gugucauacu ccuuguguac ugcagcguuc acauucacca agaucccggc ugaaacacug     1260

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1320

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1380

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1440

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1500

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1560

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1620

aagggcaucc aucaaaucuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1680

uucucacaaa uucucauugg aacguugcug augugguugg gucugaacgc aaagaaugga     1740

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     1800

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10035
<211>  1999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 wild type

<400>  10035
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ccgugacgcu cccuucucac ucuacaagga aguugcaaac gcggucgcag      120

accugguuag aaucaagaga auacacgaag cacuugauca agguugaaaa cuggauauuc      180

aggaaccccg gguuugcgcu aguggccguu gccauugccu ggcuuuuggg aagcucgacg      240

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua caguaucagg      300

ugcauuggag ucagcaauag agacuucgug gagggcaugu caggugggac cuggguugau      360

guugucuugg aacauggagg cugcguuacc gugauggcac aggacaagcc aacagucgac      420

auagaguugg ucacgacgac gguuaguaac auggccgagg uaagauccua uugcuacgag      480

gcaucgauau cggacauggc uucggacagu cguugcccaa cacaagguga agccuaccuu      540

gacaagcaau cagacacuca auaugucugc aaaagaacau uaguggacag agguugggga      600

aacgguugug gacuuuuugg caaagggagc uuggugacau gugccaaguu uacguguucu      660

aagaagauga ccgggaagag cauucaaccg gaaaaucugg aguaucggau aaugcuauca      720

gugcauggcu cccagcauag cgggaugauu ggauaugaaa cugacgaaga uagagcgaaa      780

gucgagguua cgccuaauuc accaagagcg gaagcaaccu ugggaggcuu uggaaguuua      840

ggacuugacu gugaaccaag gacaggccuu gacuuuucag aucuguauua ccugaccaug      900

aacaauaagc auugguuggu gcacaaagag ugguuucaug acaucccauu gccuuggcau      960

gcuggggcag acaccggaac uccacacugg aacaacaaag aggcauuggu agaauuuaag     1020

gaugcccacg ccaagaggca aaccgucguc guucugggga gccaggaagg agccguucac     1080

acggcucucg cuggagcucu agaggcugag auggauggug caaagggaag gcuguucucu     1140

ggccauuuga aaugccgccu aaaaauggac aagcucagau ugaagggcgu gucauauucc     1200

uugugcacug cggcauucac auucaccaag gucccagcug aaacacugca uggaacaguc     1260

acaguggagg ugcaguaugc agggacagau ggacccugca agaucccagu ccagauggcg     1320

guggacaugc agacccugac cccaguugga aggcugauaa ccgccaaccc cgugauuacu     1380

gaaagcacug agaacucaaa gaugauguug gagcuugacc caccauuugg ggauucuuac     1440

auugucauag gaguugggga caagaaaauc acccaccacu ggcauaggag ugguagcacc     1500

aucggaaagg cauuugaggc cacugugaga ggcgccaaga gaauggcagu ccugggggau     1560

acagccuggg acuucggauc agucgggggu guguucaacu cacuggguaa gggcauucac     1620

cagauuuuug gagcagccuu caaaucacug uuuggaggaa uguccugguu cucacagauc     1680

cucauaggca cgcugcuagu gugguuaggu uugaacacaa agaauggauc uaucucccuc     1740

acaugcuugg cccugggggg agugaugauc uuccucucca cggcuguuuc ugcuugagga     1800

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     1860

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     1980

uuucagagcc accagaauu                                                  1999


<210>  10036
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 wild type

<400>  10036
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg cugugacgcu ccccucccau uccacuagga agcugcaaac gcggucgcaa      120

accugguugg aaucaagaga auacacaaag cacuugauua gagucgaaaa uuggauauuc      180

aggaacccug gcuucgcguu agcagcagcu gccaucgcuu ggcuuuuggg aagcucaacg      240

agccaaaaag ucauauacuu ggucaugaua cugcugauug ccccggcaua cagcaucagg      300

ugcauaggag ucagcaauag ggacuuugug gaagguaugu caggugggac uuggguugau      360

guugucuugg aacauggagg uugugucacc guaauggcac aggacaaacc gacugucgac      420

auagagcugg uuacaacaac agucagcaac auggcggagg uaagauccua cugcuaugag      480

gcaucaauau cagacauggc uucggacagc cgcugcccaa cacaagguga agccuaccuu      540

gacaagcaau cagacacuca auaugucugc aaaagaacgu uaguggacag aggcugggga      600

aauggaugug gacuuuuugg caaagggagc cuggugacau gcgcuaaguu ugcaugcucc      660

aagaaaauga ccgggaagag cauccagcca gagaaucugg aguaccggau aaugcuguca      720

guucauggcu cccagcacag ugggaugauc guuaaugaca caggacauga aacugaugag      780

aauagagcga agguugagau aacgcccaau ucaccaagag ccgaagccac ccuggggggu      840

uuuggaagcc uaggacuuga uugugaaccg aggacaggcc uugacuuuuc agauuuguau      900

uacuugacua ugaauaacaa gcacugguug guccacaagg agugguucca cgacauucca      960

uuaccuuggc acgcuggggc agacaccgga acuccacacu ggaacaacaa agaagcacug     1020

guagaguuca aggacgcaca ugccaaaagg caaacugucg ugguucuagg gagucaagaa     1080

ggagcaguuc acacggcccu ugcuggagcu cuggaggcug agauggaugg ugcaaaggga     1140

aggcuguccu cuggccacuu gaaaugucgc cugaaaaugg auaaacuuag auugaagggc     1200

gugucauacu ccuuguguac cgcagcguuc acauucacca agaucccggc ugaaacacug     1260

cacgggacag ucacagugga gguacaguac gcagggacag auggaccuug caagguucca     1320

gcucagaugg cgguggacau gcaaacucug accccaguug ggagguugau aaccgcuaac     1380

cccguaauca cugaaagcac ugagaacucu aagaugaugc uggaacuuga uccaccauuu     1440

ggggacucuu acauugucau aggagucggg gagaagaaga ucacccacca cuggcacagg     1500

aguggcagca ccauuggaaa agcauuugaa gccacuguga gaggugccaa gagaauggca     1560

gucuugggag acacagccug ggacuuugga ucaguuggag gcgcucucaa cucauugggc     1620

aagggcaucc aucaaauuuu uggagcagcu uucaaaucau uguuuggagg aauguccugg     1680

uucucacaaa uccucauugg aacguugcug augugguugg gucugaacac aaagaaugga     1740

ucuauuuccc uuaugugcuu ggccuuaggg ggaguguuga ucuucuuauc cacagccguc     1800

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10037
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 wild type

<400>  10037
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccgcu gugacgcucc ccucccauuc cacuaggaag      120

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      180

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      240

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      300

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      360

ggugggacuu ggguugauau ugucuuggaa cauggagguu gugucaccgu aauggcacag      420

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      480

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      540

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      600

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagucu ggugacaugc      660

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      720

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      780

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      840

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      900

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ucacaaggag      960

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1020

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1080

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1140

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1200

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1260

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1320

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1380

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1440

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1500

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1560

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1620

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1680

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     1740

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     1800

uucuuaucca cagccgucuc ugcuugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10038
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 wild type

<400>  10038
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccgcu gugacgcucc ccucccauuc cacuaggaag      120

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      180

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      240

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      300

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      360

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucacugu aauggcacag      420

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      480

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      540

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      600

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      660

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      720

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      780

ggacaugaaa cugaugagaa uagagcgaaa guugagauaa cgcccaauuc accaagagcc      840

gaagccaccc uggggggguu uggaagccua ggacuugauu gugaaccgag gacaggccuu      900

gacuuuucag auuuguauua cuugacuaug aauaacaagc acuggcuggu ucacaaggag      960

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1020

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1080

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1140

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1200

aaacuuagau ugaagggcgu gucauacucc uuguguacug cagcguucac auucaccaag     1260

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1320

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1380

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1440

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1500

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1560

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1620

gcucucaacu cauugggcaa gggcauccau caaaucuuug gagcagcuuu caaaucauug     1680

uuuggaggaa uguccugguu cucacaaauu cucauuggaa cguugcugau gugguugggu     1740

cugaacgcaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     1800

uucuuaucca cagccgucuc ugcuugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10039
<211>  2017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 wild type

<400>  10039
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccgcc gugacgcucc cuucucacuc uacaaggaag      120

uugcaaacgc ggucgcagac cugguuagaa ucaagagaau acacgaagca cuugaucaag      180

guugaaaacu ggauauucag gaaccccggg uuugcgcuag uggccguugc cauugccugg      240

cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      300

ccggcauaca guaucaggug cauuggaguc agcaauagag acuucgugga gggcauguca      360

ggugggaccu ggguugaugu ugucuuggaa cauggaggcu gcguuaccgu gauggcacag      420

gacaagccaa cagucgacau agaguugguc acgacgacgg uuaguaacau ggccgaggua      480

agauccuauu gcuacgaggc aucgauaucg gacauggcuu cggacagucg uugcccaaca      540

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacauua      600

guggacagag guuggggaaa cgguugugga cuuuuuggca aagggagcuu ggugacaugu      660

gccaaguuua cguguucuaa gaagaugacc gggaagagca uucaaccgga aaaucuggag      720

uaucggauaa ugcuaucagu gcauggcucc cagcauagcg ggaugauugg auaugaaacu      780

gacgaagaua gagcgaaagu cgagguuacg ccuaauucac caagagcgga agcaaccuug      840

ggaggcuuug gaaguuuagg acuugacugu gaaccaagga caggccuuga cuuuucagau      900

cuguauuacc ugaccaugaa caauaagcau ugguuggugc acaaagagug guuucaugac      960

aucccauugc cuuggcaugc uggggcagac accggaacuc cacacuggaa caacaaagag     1020

gcauugguag aauuuaagga ugcccacgcc aagaggcaaa ccgucgucgu ucuggggagc     1080

caggaaggag ccguucacac ggcucucgcu ggagcucuag aggcugagau ggauggugca     1140

aagggaaggc uguucucugg ccauuugaaa ugccgccuaa aaauggacaa gcucagauug     1200

aagggcgugu cauauuccuu gugcacugcg gcauucacau ucaccaaggu cccagcugaa     1260

acacugcaug gaacagucac aguggaggug caguaugcag ggacagaugg acccugcaag     1320

aucccagucc agauggcggu ggacaugcag acccugaccc caguuggaag gcugauaacc     1380

gccaaccccg ugauuacuga aagcacugag aacucaaaga ugauguugga gcuugaccca     1440

ccauuugggg auucuuacau ugucauagga guuggggaca agaaaaucac ccaccacugg     1500

cauaggagug guagcaccau cggaaaggca uuugaggcca cugugagagg cgccaagaga     1560

auggcagucc ugggggauac agccugggac uucggaucag ucgggggugu guucaacuca     1620

cuggguaagg gcauucacca gauuuuugga gcagccuuca aaucacuguu uggaggaaug     1680

uccugguucu cacagauccu cauaggcacg cugcuagugu gguuagguuu gaacacaaag     1740

aauggaucua ucucccucac augcuuggcc cuggggggag ugaugaucuu ccucuccacg     1800

gcuguuucug cuugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     1860

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1980

ccccccccca aaggcucuuu ucagagccac cagaauu                              2017


<210>  10040
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 wild type

<400>  10040
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccgcu gugacgcucc ccucccauuc cacuaggaag      120

cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau acacaaagca cuugauuaga      180

gucgaaaauu ggauauucag gaacccuggc uucgcguuag cagcagcugc caucgcuugg      240

cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg ucaugauacu gcugauugcc      300

ccggcauaca gcaucaggug cauaggaguc agcaauaggg acuuugugga agguauguca      360

ggugggacuu ggguugaugu ugucuuggaa cauggagguu gugucaccgu aauggcacag      420

gacaaaccga cugucgacau agagcugguu acaacaacag ucagcaacau ggcggaggua      480

agauccuacu gcuaugaggc aucaauauca gacauggcuu cggacagccg cugcccaaca      540

caaggugaag ccuaccuuga caagcaauca gacacucaau augucugcaa aagaacguua      600

guggacagag gcuggggaaa uggaugugga cuuuuuggca aagggagccu ggugacaugc      660

gcuaaguuug caugcuccaa gaaaaugacc gggaagagca uccagccaga gaaucuggag      720

uaccggauaa ugcugucagu ucauggcucc cagcacagug ggaugaucgu uaaugacaca      780

ggacaugaaa cugaugagaa uagagcgaag guugagauaa cgcccaauuc accaagagcc      840

gaagccaccc ugggggguuu uggaagccua ggacuugauu gugaaccgag gacaggccuu      900

gacuuuucag auuuguauua cuugacuaug aauaacaagc acugguuggu ccacaaggag      960

ugguuccacg acauuccauu accuuggcac gcuggggcag acaccggaac uccacacugg     1020

aacaacaaag aagcacuggu agaguucaag gacgcacaug ccaaaaggca aacugucgug     1080

guucuaggga gucaagaagg agcaguucac acggcccuug cuggagcucu ggaggcugag     1140

auggauggug caaagggaag gcuguccucu ggccacuuga aaugucgccu gaaaauggau     1200

aaacuuagau ugaagggcgu gucauacucc uuguguaccg cagcguucac auucaccaag     1260

aucccggcug aaacacugca cgggacaguc acaguggagg uacaguacgc agggacagau     1320

ggaccuugca agguuccagc ucagauggcg guggacaugc aaacucugac cccaguuggg     1380

agguugauaa ccgcuaaccc cguaaucacu gaaagcacug agaacucuaa gaugaugcug     1440

gaacuugauc caccauuugg ggacucuuac auugucauag gagucgggga gaagaagauc     1500

acccaccacu ggcacaggag uggcagcacc auuggaaaag cauuugaagc cacugugaga     1560

ggugccaaga gaauggcagu cuugggagac acagccuggg acuuuggauc aguuggaggc     1620

gcucucaacu cauugggcaa gggcauccau caaauuuuug gagcagcuuu caaaucauug     1680

uuuggaggaa uguccugguu cucacaaauc cucauuggaa cguugcugau gugguugggu     1740

cugaacacaa agaauggauc uauuucccuu augugcuugg ccuuaggggg aguguugauc     1800

uucuuaucca cagccgucuc ugcuugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10041
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10041
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gucacccggc gcggcuccgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10042
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10042
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gucacccggc gcggcuccgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacacgugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacg      300

agcaccuggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacg cgcaagcugc agacccggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc cacgagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcaccugggu ggacgucgug      660

cuggagcacg ggggcugcgu gaccgucaug gcccaggaca agcccaccgu ggacaucgag      720

cucgugacga ccaccgucag caacauggcc gaggugcgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cgcaguacgu cugcaagcgc acccuggugg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccguccac     1020

ggcagccagc acuccgggau gaucgugaac gacacgggcc acgagaccga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacg ggccuggacu ucagcgaccu guacuaccuc     1200

accaugaaca acaagcacug gcugguccac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucguggag     1320

uucaaggacg cgcacgccaa gcgccagacg guggucgugc ugggcuccca ggagggggcc     1380

gugcacaccg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggucagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac gcuccacggc     1560

accgugaccg uggaggucca guacgccggg accgacggcc cgugcaaggu gcccgcccag     1620

auggccgugg acaugcagac gcugaccccc gucgggcgcc ugaucaccgc gaaccccgug     1680

aucaccgaga gcacggagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu cggggagaag aagaucaccc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc gugcgcggcg ccaagcggau ggccgugcug     1860

ggggacacgg cgugggacuu cggcagcguc ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacccu gcugaugugg cucgggcuga acgccaagaa cggcuccauc     2040

agccugaugu gccucgcgcu gggcggcgug cugaucuucc ucuccaccgc cgugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10043
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10043
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc aucacccgcc ggggguccgc cuacuacaug      120

uaccuggacc gcagcgacgc cggcaaggcc aucuccuucg cgaccacgcu gggcgugaac      180

aagugccacg ugcagaucau ggaccucggg cacaugugcg acgccaccau gagcuacgag      240

ugcccgaugc uggacgaggg cgucgagccc gacgacgugg acugcuggug caacaccacc      300

uccacguggg uggucuacgg gaccugccac cacaagaagg gcgaggcccg gcgcagccgg      360

cgcgccguga cccugcccuc ccacagcacc cggaagcucc agacgcgcuc ccagaccugg      420

cuggagagcc gcgaguacac caagcaccug aucaaggugg agaacuggau cuuccggaac      480

cccggcuucg cccucgucgc gguggccauc gccuggcugc ugggguccag caccucccag      540

aaggugaucu accucgucau gauccugcug aucgccccgg ccuacagcau ccgcugcauc      600

ggcgugucca accgggacuu cguggagggg augagcggcg ggacgugggu cgacguggug      660

cucgagcacg gcggcugcgu caccgugaug gcgcaggaca agcccaccgu ggacaucgag      720

cuggucacca cgaccguguc caacauggcc gagguccgca gcuacugcua cgaggccagc      780

aucuccgaca uggccagcga cucccggugc cccacccagg gggaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagcgc acgcucgugg accggggcug ggggaacggc      900

ugcgggcugu ucggcaaggg cucccugguc acgugcgcga aguucacgug cagcaagaag      960

augaccggga aguccaucca gcccgagaac cucgaguacc gcaucaugcu gagcgugcac     1020

ggcucccagc acagcgggau gaucggcuac gagacggacg aggaccgggc caagguggag     1080

gucaccccga acuccccgcg cgccgaggcc acccugggcg gguucggcag ccucgggcug     1140

gacugcgagc cccggaccgg ccuggacuuc uccgaccucu acuaccugac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccucccgug gcacgcgggg     1260

gccgacaccg gcaccccgca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaagc gccagaccgu cguggugcug ggcagccagg agggggcggu ccacacggcc     1380

cuggccggcg cccucgaggc cgagauggac ggggcgaagg gccggcuguu cuccgggcac     1440

cugaagugcc gccucaagau ggacaagcug cggcugaagg gcgugagcua cucccucugc     1500

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccc ugcaagaucc cgguccagau ggcgguggac     1620

augcagaccc ugacgcccgu ggggcgccuc aucaccgcca accccgucau caccgagagc     1680

accgagaacu ccaagaugau gcuggagcug gacccgccgu ucggcgacag cuacaucgug     1740

aucggggugg gcgacaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1800

aaggccuucg aggccaccgu ccgcggcgcg aagcggaugg ccgugcucgg ggacaccgcc     1860

ugggacuucg gcagcguggg cggcgucuuc aacucccugg ggaaggggau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1980

gggacgcugc ugguguggcu cggccugaac accaagaacg gcuccaucag ccugaccugc     2040

cucgcgcugg gcggcgugau gaucuuccug uccaccgccg ucagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10044
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10044
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gucacccggc gcggcuccgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacgucgug      660

cuggagcacg ggggcugcgu gaccgucaug gcccaggaca agcccaccgu ggacaucgag      720

cucgugacca cgaccgucag caacauggcc gaggugcgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu cugcaagcgc acgcuggugg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccguccac     1020

ggcagccagc acuccgggau gaucgugaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcugguccac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucguggag     1320

uucaaggacg cgcacgccaa gcgccagacc guggucgugc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggucagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggugaccg uggaggucca guacgccggg accgacggcc cgugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugacgccc gucgggcgcc ugaucaccgc gaaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu cggggagaag aagaucacgc accacuggca ccggagcggc     1800

uccaccaucg ggaaggccuu cgaggccacc gugcgcggcg ccaagcggau ggccgugcug     1860

ggggacaccg cgugggacuu cggcagcguc ggcggggccc ucaacucccu gggcaagggg     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1980

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     2040

agccugaugu gccucgcccu gggcggcgug cugaucuucc ucuccaccgc ggugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10045
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10045
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg uccgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgugg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgucc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10046
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10046
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacacggc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacccug     1980

cugauguggc ucgggcugaa cgccaagaac ggcuccauca gccugaugug ccucgcgcug     2040

ggcggcgugc ugaucuuccu cuccaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10047
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10047
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagca ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguu cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1800

cgcggcgcga agcggauggc cgugcucggg gacaccgccu gggacuucgg cagcgugggc     1860

ggcgucuuca acucccuggg gaaggggauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucgggg gcauguccug guucagccag auccucaucg ggacgcugcu gguguggcuc     1980

ggccugaaca ccaagaacgg cuccaucagc cugaccugcc ucgcgcuggg cggcgugaug     2040

aucuuccugu ccaccgccgu cagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10048
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10048
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggcu ccaccaucgg gaaggccuuc     1800

gaggccaccg ugcgcggcgc caagcggaug gccgugcugg gggacaccgc gugggacuuc     1860

ggcagcgucg gcggggcccu caacucccug ggcaagggga uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg gggcaugucc ugguucagcc agauccucau cggcacgcug     1980

cugauguggc ucgggcugaa caccaagaac ggcuccauca gccugaugug ccucgcccug     2040

ggcggcgugc ugaucuuccu cuccaccgcg gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10049
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10049
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccguc acccggcgcg gcuccgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaaga gcauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccguc cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgugggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcguccug aucuuccucu ccaccgcggu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10050
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10050
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccguc acccggcgcg gcuccgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac acgugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccacgagc      300

accugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacgcgc aagcugcaga cccggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac gagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca ccugggugga cgucgugcug      660

gagcacgggg gcugcgugac cgucauggcc caggacaagc ccaccgugga caucgagcuc      720

gugacgacca ccgucagcaa cauggccgag gugcgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacacgc aguacgucug caagcgcacc cugguggacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaaga gcauccagcc ggagaaccug gaguaccgca ucaugcucuc cguccacggc     1020

agccagcacu ccgggaugau cgugaacgac acgggccacg agaccgacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggacgggc cuggacuuca gcgaccugua cuaccucacc     1200

augaacaaca agcacuggcu gguccacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cguggaguuc     1320

aaggacgcgc acgccaagcg ccagacggug gucgugcugg gcucccagga gggggccgug     1380

cacaccgccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggucagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacgcu ccacggcacc     1560

gugaccgugg agguccagua cgccgggacc gacggcccgu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacgcu gacccccguc gggcgccuga ucaccgcgaa ccccgugauc     1680

accgagagca cggagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcgucgg ggagaagaag aucacccacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccgug cgcggcgcca agcggauggc cgugcugggg     1860

gacacggcgu gggacuucgg cagcgucggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacccugcu gauguggcuc gggcugaacg ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcgcuggg cggcgugcug aucuuccucu ccaccgccgu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10051
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10051
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccauc acccgccggg gguccgccua cuacauguac      120

cuggaccgca gcgacgccgg caaggccauc uccuucgcga ccacgcuggg cgugaacaag      180

ugccacgugc agaucaugga ccucgggcac augugcgacg ccaccaugag cuacgagugc      240

ccgaugcugg acgagggcgu cgagcccgac gacguggacu gcuggugcaa caccaccucc      300

acgugggugg ucuacgggac cugccaccac aagaagggcg aggcccggcg cagccggcgc      360

gccgugaccc ugcccuccca cagcacccgg aagcuccaga cgcgcuccca gaccuggcug      420

gagagccgcg aguacaccaa gcaccugauc aagguggaga acuggaucuu ccggaacccc      480

ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg gguccagcac cucccagaag      540

gugaucuacc ucgucaugau ccugcugauc gccccggccu acagcauccg cugcaucggc      600

guguccaacc gggacuucgu ggaggggaug agcggcggga cgugggucga cguggugcuc      660

gagcacggcg gcugcgucac cgugauggcg caggacaagc ccaccgugga caucgagcug      720

gucaccacga ccguguccaa cauggccgag guccgcagcu acugcuacga ggccagcauc      780

uccgacaugg ccagcgacuc ccggugcccc acccaggggg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagcgcacg cucguggacc ggggcugggg gaacggcugc      900

gggcuguucg gcaagggcuc ccuggucacg ugcgcgaagu ucacgugcag caagaagaug      960

accgggaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcugag cgugcacggc     1020

ucccagcaca gcgggaugau cggcuacgag acggacgagg accgggccaa gguggagguc     1080

accccgaacu ccccgcgcgc cgaggccacc cugggcgggu ucggcagccu cgggcuggac     1140

ugcgagcccc ggaccggccu ggacuucucc gaccucuacu accugacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ucccguggca cgcgggggcc     1260

gacaccggca ccccgcacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu ggugcugggc agccaggagg gggcggucca cacggcccug     1380

gccggcgccc ucgaggccga gauggacggg gcgaagggcc ggcuguucuc cgggcaccug     1440

aagugccgcc ucaagaugga caagcugcgg cugaagggcg ugagcuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa ggugcccgcc gagacccugc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggccccugc aagaucccgg uccagauggc gguggacaug     1620

cagacccuga cgcccguggg gcgccucauc accgccaacc ccgucaucac cgagagcacc     1680

gagaacucca agaugaugcu ggagcuggac ccgccguucg gcgacagcua caucgugauc     1740

ggggugggcg acaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag     1800

gccuucgagg ccaccguccg cggcgcgaag cggauggccg ugcucgggga caccgccugg     1860

gacuucggca gcgugggcgg cgucuucaac ucccugggga aggggaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggg     1980

acgcugcugg uguggcucgg ccugaacacc aagaacggcu ccaucagccu gaccugccuc     2040

gcgcugggcg gcgugaugau cuuccugucc accgccguca gcgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10052
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10052
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccguc acccggcgcg gcuccgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga cgucgugcug      660

gagcacgggg gcugcgugac cgucauggcc caggacaagc ccaccgugga caucgagcuc      720

gugaccacga ccgucagcaa cauggccgag gugcgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgucug caagcgcacg cugguggacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaaga gcauccagcc ggagaaccug gaguaccgca ucaugcucuc cguccacggc     1020

agccagcacu ccgggaugau cgugaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu gguccacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cguggaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gucgugcugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggucagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gugaccgugg agguccagua cgccgggacc gacggcccgu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccguc gggcgccuga ucaccgcgaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcgucgg ggagaagaag aucacgcacc acuggcaccg gagcggcucc     1800

accaucggga aggccuucga ggccaccgug cgcggcgcca agcggauggc cgugcugggg     1860

gacaccgcgu gggacuucgg cagcgucggc ggggcccuca acucccuggg caaggggauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucgggg gcauguccug guucagccag     1980

auccucaucg gcacgcugcu gauguggcuc gggcugaaca ccaagaacgg cuccaucagc     2040

cugaugugcc ucgcccuggg cggcgugcug aucuuccucu ccaccgcggu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10053
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10053
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gucacccggc gcggcuccgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      660

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      720

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac     1020

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1620

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1800

agcacccugg ggaaggccuu cuccaccacg cucaagggcg cccagcgccu ggcggcccug     1860

ggggacaccg ccugggacuu cggcagcauc gggggcgugu ucaacuccau cggccgggcc     1920

guccaccagg uguucggggg cgccuuccgc acccucuucg ggggcaugag cuggaucacc     1980

caggggcuga ugggcgcgcu gcuccugugg auggggguga acgcccggga ccgcuccauc     2040

gcccuggccu uccucgcgac gggcggcguc cugguguucc uggccaccaa cgugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10054
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10054
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gucacccggc gcggcuccgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacacgugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacg      300

agcaccuggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacg cgcaagcugc agacccggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc cacgagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcaccugggu ggacgucgug      660

cuggagcacg ggggcugcgu gaccgucaug gcccaggaca agcccaccgu ggacaucgag      720

cucgugacga ccaccgucag caacauggcc gaggugcgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cgcaguacgu cugcaagcgc acccuggugg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccguccac     1020

ggcagccagc acuccgggau gaucgugaac gacacgggcc acgagaccga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacg ggccuggacu ucagcgaccu guacuaccuc     1200

accaugaaca acaagcacug gcugguccac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucguggag     1320

uucaaggacg cgcacgccaa gcgccagacg guggucgugc ugggcuccca ggagggggcc     1380

gugcacaccg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggucagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac gcuccacggc     1560

accgugaccg uggaggucca guacgccggg accgacggcc cgugcaaggu gcccgcccag     1620

auggccgugg acaugcagac gcugaccccc gucgggcgcc ugaucaccgc gaaccccgug     1680

aucaccgaga gcacggagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu cggggagaag aagaucaccc accacuggca ccggagcggc     1800

agcacccugg ggaaggccuu cuccaccacg cucaagggcg cccagcgccu ggcggcccug     1860

ggggacaccg ccugggacuu cggcagcauc gggggcgugu ucaacuccau cggccgggcc     1920

guccaccagg uguucggggg cgccuuccgc acccucuucg ggggcaugag cuggaucacc     1980

caggggcuga ugggcgcgcu gcuccugugg auggggguga acgcccggga ccgcuccauc     2040

gcccuggccu uccucgcgac gggcggcguc cugguguucc uggccaccaa cgugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10055
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10055
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc aucacccgcc ggggguccgc cuacuacaug      120

uaccuggacc gcagcgacgc cggcaaggcc aucuccuucg cgaccacgcu gggcgugaac      180

aagugccacg ugcagaucau ggaccucggg cacaugugcg acgccaccau gagcuacgag      240

ugcccgaugc uggacgaggg cgucgagccc gacgacgugg acugcuggug caacaccacc      300

uccacguggg uggucuacgg gaccugccac cacaagaagg gcgaggcccg gcgcagccgg      360

cgcgccguga cccugcccuc ccacagcacc cggaagcucc agacgcgcuc ccagaccugg      420

cuggagagcc gcgaguacac caagcaccug aucaaggugg agaacuggau cuuccggaac      480

cccggcuucg cccucgucgc gguggccauc gccuggcugc ugggguccag caccucccag      540

aaggugaucu accucgucau gauccugcug aucgccccgg ccuacagcau ccgcugcauc      600

ggcgugucca accgggacuu cguggagggg augagcggcg ggacgugggu cgacguggug      660

cucgagcacg gcggcugcgu caccgugaug gcgcaggaca agcccaccgu ggacaucgag      720

cuggucacca cgaccguguc caacauggcc gagguccgca gcuacugcua cgaggccagc      780

aucuccgaca uggccagcga cucccggugc cccacccagg gggaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagcgc acgcucgugg accggggcug ggggaacggc      900

ugcgggcugu ucggcaaggg cucccugguc acgugcgcga aguucacgug cagcaagaag      960

augaccggga aguccaucca gcccgagaac cucgaguacc gcaucaugcu gagcgugcac     1020

ggcucccagc acagcgggau gaucggcuac gagacggacg aggaccgggc caagguggag     1080

gucaccccga acuccccgcg cgccgaggcc acccugggcg gguucggcag ccucgggcug     1140

gacugcgagc cccggaccgg ccuggacuuc uccgaccucu acuaccugac gaugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccucccgug gcacgcgggg     1260

gccgacaccg gcaccccgca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaagc gccagaccgu cguggugcug ggcagccagg agggggcggu ccacacggcc     1380

cuggccggcg cccucgaggc cgagauggac ggggcgaagg gccggcuguu cuccgggcac     1440

cugaagugcc gccucaagau ggacaagcug cggcugaagg gcgugagcua cucccucugc     1500

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac ggucaccgug     1560

gaggugcagu acgccgggac cgacggcccc ugcaagaucc cgguccagau ggcgguggac     1620

augcagaccc ugacgcccgu ggggcgccuc aucaccgcca accccgucau caccgagagc     1680

accgagaacu ccaagaugau gcuggagcug gacccgccgu ucggcgacag cuacaucgug     1740

aucggggugg gcgacaagaa gaucacgcac cacuggcacc ggagcggcag cacccugggg     1800

aaggccuucu ccaccacgcu caagggcgcc cagcgccugg cggcccuggg ggacaccgcc     1860

ugggacuucg gcagcaucgg gggcguguuc aacuccaucg gccgggccgu ccaccaggug     1920

uucgggggcg ccuuccgcac ccucuucggg ggcaugagcu ggaucaccca ggggcugaug     1980

ggcgcgcugc uccuguggau gggggugaac gcccgggacc gcuccaucgc ccuggccuuc     2040

cucgcgacgg gcggcguccu gguguuccug gccaccaacg ugcacgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10056
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10056
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gucacccggc gcggcuccgc cuacuacaug      120

uaccucgacc ggaacgacgc cggggaggcc aucagcuucc cgaccacgcu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggg cacaugugcg acgcgaccau guccuacgag      240

ugccccaugc ucgacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcacguggg ucguguacgg cacgugccac cacaagaagg gggaggcccg ccggucccgc      360

cgggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      420

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      480

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      540

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      600

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacgucgug      660

cuggagcacg ggggcugcgu gaccgucaug gcccaggaca agcccaccgu ggacaucgag      720

cucgugacca cgaccgucag caacauggcc gaggugcgca gcuacugcua cgaggccucc      780

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      840

caguccgaca cccaguacgu cugcaagcgc acgcuggugg accggggcug ggggaacggc      900

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      960

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccguccac     1020

ggcagccagc acuccgggau gaucgugaac gacaccggcc acgagacgga cgagaaccgg     1080

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc     1140

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc     1200

acgaugaaca acaagcacug gcugguccac aaggaguggu uccacgacau cccgcugccc     1260

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucguggag     1320

uucaaggacg cgcacgccaa gcgccagacc guggucgugc ugggcuccca ggagggggcc     1380

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1440

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggucagc     1500

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1560

acggugaccg uggaggucca guacgccggg accgacggcc cgugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugacgccc gucgggcgcc ugaucaccgc gaaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1740

uccuacaucg ugaucggcgu cggggagaag aagaucacgc accacuggca ccggagcggc     1800

agcacccugg ggaaggccuu cuccaccacg cucaagggcg cccagcgccu ggcggcccug     1860

ggggacaccg ccugggacuu cggcagcauc gggggcgugu ucaacuccau cggccgggcc     1920

guccaccagg uguucggggg cgccuuccgc acccucuucg ggggcaugag cuggaucacc     1980

caggggcuga ugggcgcgcu gcuccugugg auggggguga acgcccggga ccgcuccauc     2040

gcccuggccu uccucgcgac gggcggcguc cugguguucc uggccaccaa cgugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10057
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10057
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacaucgucc uggagcacgg gggcugcgug      660

accgugaugg cccaggacaa gcccaccguc gacaucgagc ucgugaccac gaccgugagc      720

aacauggccg agguccgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacgug      840

ugcaagcgca cgcuggucga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccgugcacg gcagccagca cuccgggaug     1020

aucgucaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggugguccu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggugagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggucaccgu ggaggugcag     1560

uacgccggga ccgacggccc gugcaagguc cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg uggggcgccu gaucaccgcg aaccccguca ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcgug     1740

ggggagaaga agaucacgca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10058
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10058
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acacgugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacga gcaccugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccacgc gcaagcugca gacccggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc acgagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg caccugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugacgac caccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac gcaguacguc      840

ugcaagcgca cccuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acacgggcca cgagaccgac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggacgg gccuggacuu cagcgaccug uacuaccuca ccaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagacgg uggucgugcu gggcucccag gagggggccg ugcacaccgc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacg cuccacggca ccgugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacg     1620

cugacccccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag cacggagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucaccca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10059
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10059
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagca ucacccgccg gggguccgcc uacuacaugu accuggaccg cagcgacgcc      120

ggcaaggcca ucuccuucgc gaccacgcug ggcgugaaca agugccacgu gcagaucaug      180

gaccucgggc acaugugcga cgccaccaug agcuacgagu gcccgaugcu ggacgagggc      240

gucgagcccg acgacgugga cugcuggugc aacaccaccu ccacgugggu ggucuacggg      300

accugccacc acaagaaggg cgaggcccgg cgcagccggc gcgccgugac ccugcccucc      360

cacagcaccc ggaagcucca gacgcgcucc cagaccuggc uggagagccg cgaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uuccggaacc ccggcuucgc ccucgucgcg      480

guggccaucg ccuggcugcu gggguccagc accucccaga aggugaucua ccucgucaug      540

auccugcuga ucgccccggc cuacagcauc cgcugcaucg gcguguccaa ccgggacuuc      600

guggagggga ugagcggcgg gacguggguc gacguggugc ucgagcacgg cggcugcguc      660

accgugaugg cgcaggacaa gcccaccgug gacaucgagc uggucaccac gaccgugucc      720

aacauggccg agguccgcag cuacugcuac gaggccagca ucuccgacau ggccagcgac      780

ucccggugcc ccacccaggg ggaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagcgca cgcucgugga ccggggcugg gggaacggcu gcgggcuguu cggcaagggc      900

ucccugguca cgugcgcgaa guucacgugc agcaagaaga ugaccgggaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcug agcgugcacg gcucccagca cagcgggaug     1020

aucggcuacg agacggacga ggaccgggcc aagguggagg ucaccccgaa cuccccgcgc     1080

gccgaggcca cccugggcgg guucggcagc cucgggcugg acugcgagcc ccggaccggc     1140

cuggacuucu ccgaccucua cuaccugacg augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccucccgugg cacgcggggg ccgacaccgg caccccgcac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagcg ccagaccguc     1320

guggugcugg gcagccagga gggggcgguc cacacggccc uggccggcgc ccucgaggcc     1380

gagauggacg gggcgaaggg ccggcuguuc uccgggcacc ugaagugccg ccucaagaug     1440

gacaagcugc ggcugaaggg cgugagcuac ucccucugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacg gucaccgugg aggugcagua cgccgggacc     1560

gacggccccu gcaagauccc gguccagaug gcgguggaca ugcagacccu gacgcccgug     1620

gggcgccuca ucaccgccaa ccccgucauc accgagagca ccgagaacuc caagaugaug     1680

cuggagcugg acccgccguu cggcgacagc uacaucguga ucgggguggg cgacaagaag     1740

aucacgcacc acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc     1800

aagggcgccc agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg     1860

ggcguguuca acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc     1920

cucuucgggg gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug     1980

ggggugaacg cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug     2040

guguuccugg ccaccaacgu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10060
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10060
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacccggcg cggcuccgcc uacuacaugu accucgaccg gaacgacgcc      120

ggggaggcca ucagcuuccc gaccacgcug ggcaugaaca agugcuacau ccagaucaug      180

gaccuggggc acaugugcga cgcgaccaug uccuacgagu gccccaugcu cgacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcacgugggu cguguacggc      300

acgugccacc acaagaaggg ggaggcccgc cggucccgcc gggccgugac ccugcccagc      360

cacuccaccc gcaagcugca gacgcggagc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuga uccgggucga gaacuggauc uuccgcaacc cgggcuucgc ccuggccgcg      480

gccgccaucg ccuggcuccu ggggagcucc accagccaga aggugaucua ccuggugaug      540

auccuccuga ucgcccccgc guacuccauc cgcugcaucg gcgucagcaa ccgggacuuc      600

guggagggga uguccggcgg cacgugggug gacgucgugc uggagcacgg gggcugcgug      660

accgucaugg cccaggacaa gcccaccgug gacaucgagc ucgugaccac gaccgucagc      720

aacauggccg aggugcgcag cuacugcuac gaggccucca ucagcgacau ggccuccgac      780

agccggugcc ccacccaggg ggaggcguac cuggacaagc aguccgacac ccaguacguc      840

ugcaagcgca cgcuggugga ccggggcugg gggaacggcu gcggccucuu cgggaagggc      900

agccugguga ccugcgccaa guucgccugc uccaagaaga ugaccgggaa gagcauccag      960

ccggagaacc uggaguaccg caucaugcuc uccguccacg gcagccagca cuccgggaug     1020

aucgugaacg acaccggcca cgagacggac gagaaccggg ccaaggugga gaucaccccc     1080

aacagccccc gcgcggaggc cacccugggc ggguucggcu cccuggggcu cgacugcgag     1140

ccccggaccg gccuggacuu cagcgaccug uacuaccuca cgaugaacaa caagcacugg     1200

cugguccaca aggagugguu ccacgacauc ccgcugcccu ggcacgccgg cgccgacacc     1260

gggaccccgc acuggaacaa caaggaggcc cucguggagu ucaaggacgc gcacgccaag     1320

cgccagaccg uggucgugcu gggcucccag gagggggccg ugcacacggc ccuggccggc     1380

gcgcuggagg ccgagaugga cggggccaag ggccggcuca gcuccggcca ccugaagugc     1440

cgccugaaga uggacaagcu ccggcugaag ggggucagcu acucccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgcggagacc cuccacggca cggugaccgu ggagguccag     1560

uacgccggga ccgacggccc gugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacgcccg ucgggcgccu gaucaccgcg aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcucgagcu ggacccgccc uucggcgacu ccuacaucgu gaucggcguc     1740

ggggagaaga agaucacgca ccacuggcac cggagcggca gcacccuggg gaaggccuuc     1800

uccaccacgc ucaagggcgc ccagcgccug gcggcccugg gggacaccgc cugggacuuc     1860

ggcagcaucg ggggcguguu caacuccauc ggccgggccg uccaccaggu guucgggggc     1920

gccuuccgca cccucuucgg gggcaugagc uggaucaccc aggggcugau gggcgcgcug     1980

cuccugugga ugggggugaa cgcccgggac cgcuccaucg cccuggccuu ccucgcgacg     2040

ggcggcgucc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10061
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10061
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccguc acccggcgcg gcuccgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga caucguccug      660

gagcacgggg gcugcgugac cgugauggcc caggacaagc ccaccgucga caucgagcuc      720

gugaccacga ccgugagcaa cauggccgag guccgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgugug caagcgcacg cuggucgacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaaga gcauccagcc ggagaaccug gaguaccgca ucaugcucuc cgugcacggc     1020

agccagcacu ccgggaugau cgucaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gugguccugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggugagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gucaccgugg aggugcagua cgccgggacc gacggcccgu gcaagguccc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccgug gggcgccuga ucaccgcgaa ccccgucauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcguggg ggagaagaag aucacgcacc acuggcaccg gagcggcagc     1800

acccugggga aggccuucuc caccacgcuc aagggcgccc agcgccuggc ggcccugggg     1860

gacaccgccu gggacuucgg cagcaucggg ggcguguuca acuccaucgg ccgggccguc     1920

caccaggugu ucgggggcgc cuuccgcacc cucuucgggg gcaugagcug gaucacccag     1980

gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg cccgggaccg cuccaucgcc     2040

cuggccuucc ucgcgacggg cggcguccug guguuccugg ccaccaacgu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10062
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10062
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccguc acccggcgcg gcuccgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac acgugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccacgagc      300

accugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacgcgc aagcugcaga cccggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac gagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca ccugggugga cgucgugcug      660

gagcacgggg gcugcgugac cgucauggcc caggacaagc ccaccgugga caucgagcuc      720

gugacgacca ccgucagcaa cauggccgag gugcgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacacgc aguacgucug caagcgcacc cugguggacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaaga gcauccagcc ggagaaccug gaguaccgca ucaugcucuc cguccacggc     1020

agccagcacu ccgggaugau cgugaacgac acgggccacg agaccgacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggacgggc cuggacuuca gcgaccugua cuaccucacc     1200

augaacaaca agcacuggcu gguccacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cguggaguuc     1320

aaggacgcgc acgccaagcg ccagacggug gucgugcugg gcucccagga gggggccgug     1380

cacaccgccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggucagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacgcu ccacggcacc     1560

gugaccgugg agguccagua cgccgggacc gacggcccgu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacgcu gacccccguc gggcgccuga ucaccgcgaa ccccgugauc     1680

accgagagca cggagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcgucgg ggagaagaag aucacccacc acuggcaccg gagcggcagc     1800

acccugggga aggccuucuc caccacgcuc aagggcgccc agcgccuggc ggcccugggg     1860

gacaccgccu gggacuucgg cagcaucggg ggcguguuca acuccaucgg ccgggccguc     1920

caccaggugu ucgggggcgc cuuccgcacc cucuucgggg gcaugagcug gaucacccag     1980

gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg cccgggaccg cuccaucgcc     2040

cuggccuucc ucgcgacggg cggcguccug guguuccugg ccaccaacgu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10063
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10063
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccauc acccgccggg gguccgccua cuacauguac      120

cuggaccgca gcgacgccgg caaggccauc uccuucgcga ccacgcuggg cgugaacaag      180

ugccacgugc agaucaugga ccucgggcac augugcgacg ccaccaugag cuacgagugc      240

ccgaugcugg acgagggcgu cgagcccgac gacguggacu gcuggugcaa caccaccucc      300

acgugggugg ucuacgggac cugccaccac aagaagggcg aggcccggcg cagccggcgc      360

gccgugaccc ugcccuccca cagcacccgg aagcuccaga cgcgcuccca gaccuggcug      420

gagagccgcg aguacaccaa gcaccugauc aagguggaga acuggaucuu ccggaacccc      480

ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg gguccagcac cucccagaag      540

gugaucuacc ucgucaugau ccugcugauc gccccggccu acagcauccg cugcaucggc      600

guguccaacc gggacuucgu ggaggggaug agcggcggga cgugggucga cguggugcuc      660

gagcacggcg gcugcgucac cgugauggcg caggacaagc ccaccgugga caucgagcug      720

gucaccacga ccguguccaa cauggccgag guccgcagcu acugcuacga ggccagcauc      780

uccgacaugg ccagcgacuc ccggugcccc acccaggggg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagcgcacg cucguggacc ggggcugggg gaacggcugc      900

gggcuguucg gcaagggcuc ccuggucacg ugcgcgaagu ucacgugcag caagaagaug      960

accgggaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcugag cgugcacggc     1020

ucccagcaca gcgggaugau cggcuacgag acggacgagg accgggccaa gguggagguc     1080

accccgaacu ccccgcgcgc cgaggccacc cugggcgggu ucggcagccu cgggcuggac     1140

ugcgagcccc ggaccggccu ggacuucucc gaccucuacu accugacgau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ucccguggca cgcgggggcc     1260

gacaccggca ccccgcacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu ggugcugggc agccaggagg gggcggucca cacggcccug     1380

gccggcgccc ucgaggccga gauggacggg gcgaagggcc ggcuguucuc cgggcaccug     1440

aagugccgcc ucaagaugga caagcugcgg cugaagggcg ugagcuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa ggugcccgcc gagacccugc acggcacggu caccguggag     1560

gugcaguacg ccgggaccga cggccccugc aagaucccgg uccagauggc gguggacaug     1620

cagacccuga cgcccguggg gcgccucauc accgccaacc ccgucaucac cgagagcacc     1680

gagaacucca agaugaugcu ggagcuggac ccgccguucg gcgacagcua caucgugauc     1740

ggggugggcg acaagaagau cacgcaccac uggcaccgga gcggcagcac ccuggggaag     1800

gccuucucca ccacgcucaa gggcgcccag cgccuggcgg cccuggggga caccgccugg     1860

gacuucggca gcaucggggg cguguucaac uccaucggcc gggccgucca ccagguguuc     1920

gggggcgccu uccgcacccu cuucgggggc augagcugga ucacccaggg gcugaugggc     1980

gcgcugcucc uguggauggg ggugaacgcc cgggaccgcu ccaucgcccu ggccuuccuc     2040

gcgacgggcg gcguccuggu guuccuggcc accaacgugc acgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10064
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10064
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccguc acccggcgcg gcuccgccua cuacauguac      120

cucgaccgga acgacgccgg ggaggccauc agcuucccga ccacgcuggg caugaacaag      180

ugcuacaucc agaucaugga ccuggggcac augugcgacg cgaccauguc cuacgagugc      240

cccaugcucg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

acgugggucg uguacggcac gugccaccac aagaaggggg aggcccgccg gucccgccgg      360

gccgugaccc ugcccagcca cuccacccgc aagcugcaga cgcggagcca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccugauc cgggucgaga acuggaucuu ccgcaacccg      480

ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg ggagcuccac cagccagaag      540

gugaucuacc uggugaugau ccuccugauc gcccccgcgu acuccauccg cugcaucggc      600

gucagcaacc gggacuucgu ggaggggaug uccggcggca cgugggugga cgucgugcug      660

gagcacgggg gcugcgugac cgucauggcc caggacaagc ccaccgugga caucgagcuc      720

gugaccacga ccgucagcaa cauggccgag gugcgcagcu acugcuacga ggccuccauc      780

agcgacaugg ccuccgacag ccggugcccc acccaggggg aggcguaccu ggacaagcag      840

uccgacaccc aguacgucug caagcgcacg cugguggacc ggggcugggg gaacggcugc      900

ggccucuucg ggaagggcag ccuggugacc ugcgccaagu ucgccugcuc caagaagaug      960

accgggaaga gcauccagcc ggagaaccug gaguaccgca ucaugcucuc cguccacggc     1020

agccagcacu ccgggaugau cgugaacgac accggccacg agacggacga gaaccgggcc     1080

aagguggaga ucacccccaa cagcccccgc gcggaggcca cccugggcgg guucggcucc     1140

cuggggcucg acugcgagcc ccggaccggc cuggacuuca gcgaccugua cuaccucacg     1200

augaacaaca agcacuggcu gguccacaag gagugguucc acgacauccc gcugcccugg     1260

cacgccggcg ccgacaccgg gaccccgcac uggaacaaca aggaggcccu cguggaguuc     1320

aaggacgcgc acgccaagcg ccagaccgug gucgugcugg gcucccagga gggggccgug     1380

cacacggccc uggccggcgc gcuggaggcc gagauggacg gggccaaggg ccggcucagc     1440

uccggccacc ugaagugccg ccugaagaug gacaagcucc ggcugaaggg ggucagcuac     1500

ucccugugca ccgccgccuu caccuucacc aagauccccg cggagacccu ccacggcacg     1560

gugaccgugg agguccagua cgccgggacc gacggcccgu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacgcccguc gggcgccuga ucaccgcgaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cucgagcugg acccgcccuu cggcgacucc     1740

uacaucguga ucggcgucgg ggagaagaag aucacgcacc acuggcaccg gagcggcagc     1800

acccugggga aggccuucuc caccacgcuc aagggcgccc agcgccuggc ggcccugggg     1860

gacaccgccu gggacuucgg cagcaucggg ggcguguuca acuccaucgg ccgggccguc     1920

caccaggugu ucgggggcgc cuuccgcacc cucuucgggg gcaugagcug gaucacccag     1980

gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg cccgggaccg cuccaucgcc     2040

cuggccuucc ucgcgacggg cggcguccug guguuccugg ccaccaacgu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10065
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10065
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      120

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      180

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      240

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      300

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacaucguc      360

cuggagcacg ggggcugcgu gaccgugaug gcccaggaca agcccaccgu cgacaucgag      420

cucgugacca cgaccgugag caacauggcc gagguccgca gcuacugcua cgaggccucc      480

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      540

caguccgaca cccaguacgu gugcaagcgc acgcuggucg accggggcug ggggaacggc      600

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      660

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccgugcac      720

ggcagccagc acuccgggau gaucgucaac gacaccggcc acgagacgga cgagaaccgg      780

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc      840

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc      900

acgaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccgcugccc      960

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucgucgag     1020

uucaaggacg cgcacgccaa gcgccagacc gugguggucc ugggcuccca ggagggggcc     1080

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1140

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggugagc     1200

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1260

acggucaccg uggaggugca guacgccggg accgacggcc cgugcaaggu ccccgcccag     1320

auggccgugg acaugcagac ccugacgccc guggggcgcc ugaucaccgc gaaccccguc     1380

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1440

uccuacaucg ugaucggcgu gggggagaag aagaucacgc accacuggca ccggagcggc     1500

uccaccaucg ggaaggccuu cgaggccacc guccgcggcg ccaagcggau ggccgugcug     1560

ggggacaccg cgugggacuu cggcagcgug ggcggggccc ucaacucccu gggcaagggg     1620

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1680

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     1740

agccugaugu gccucgcccu gggcggcguc cugaucuucc ucuccaccgc ggugagcgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10066
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10066
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccguga cccugcccag ccacuccacg cgcaagcugc agacccggag ccagaccugg      120

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      180

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc cacgagccag      240

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      300

ggcgucagca accgggacuu cguggagggg auguccggcg gcaccugggu ggacgucgug      360

cuggagcacg ggggcugcgu gaccgucaug gcccaggaca agcccaccgu ggacaucgag      420

cucgugacga ccaccgucag caacauggcc gaggugcgca gcuacugcua cgaggccucc      480

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      540

caguccgaca cgcaguacgu cugcaagcgc acccuggugg accggggcug ggggaacggc      600

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      660

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccguccac      720

ggcagccagc acuccgggau gaucgugaac gacacgggcc acgagaccga cgagaaccgg      780

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc      840

ucccuggggc ucgacugcga gccccggacg ggccuggacu ucagcgaccu guacuaccuc      900

accaugaaca acaagcacug gcugguccac aaggaguggu uccacgacau cccgcugccc      960

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucguggag     1020

uucaaggacg cgcacgccaa gcgccagacg guggucgugc ugggcuccca ggagggggcc     1080

gugcacaccg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1140

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggucagc     1200

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac gcuccacggc     1260

accgugaccg uggaggucca guacgccggg accgacggcc cgugcaaggu gcccgcccag     1320

auggccgugg acaugcagac gcugaccccc gucgggcgcc ugaucaccgc gaaccccgug     1380

aucaccgaga gcacggagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1440

uccuacaucg ugaucggcgu cggggagaag aagaucaccc accacuggca ccggagcggc     1500

uccaccaucg ggaaggccuu cgaggccacc gugcgcggcg ccaagcggau ggccgugcug     1560

ggggacacgg cgugggacuu cggcagcguc ggcggggccc ucaacucccu gggcaagggg     1620

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1680

cagauccuca ucggcacccu gcugaugugg cucgggcuga acgccaagaa cggcuccauc     1740

agccugaugu gccucgcgcu gggcggcgug cugaucuucc ucuccaccgc cgugagcgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10067
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10067
gggagaaagc uuaccaugac cagcaucggg aucaucggcc ugcugcucac gaccgccaug       60

gcggccguga cccugcccuc ccacagcacc cggaagcucc agacgcgcuc ccagaccugg      120

cuggagagcc gcgaguacac caagcaccug aucaaggugg agaacuggau cuuccggaac      180

cccggcuucg cccucgucgc gguggccauc gccuggcugc ugggguccag caccucccag      240

aaggugaucu accucgucau gauccugcug aucgccccgg ccuacagcau ccgcugcauc      300

ggcgugucca accgggacuu cguggagggg augagcggcg ggacgugggu cgacguggug      360

cucgagcacg gcggcugcgu caccgugaug gcgcaggaca agcccaccgu ggacaucgag      420

cuggucacca cgaccguguc caacauggcc gagguccgca gcuacugcua cgaggccagc      480

aucuccgaca uggccagcga cucccggugc cccacccagg gggaggccua ccuggacaag      540

cagagcgaca cccaguacgu gugcaagcgc acgcucgugg accggggcug ggggaacggc      600

ugcgggcugu ucggcaaggg cucccugguc acgugcgcga aguucacgug cagcaagaag      660

augaccggga aguccaucca gcccgagaac cucgaguacc gcaucaugcu gagcgugcac      720

ggcucccagc acagcgggau gaucggcuac gagacggacg aggaccgggc caagguggag      780

gucaccccga acuccccgcg cgccgaggcc acccugggcg gguucggcag ccucgggcug      840

gacugcgagc cccggaccgg ccuggacuuc uccgaccucu acuaccugac gaugaacaac      900

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccucccgug gcacgcgggg      960

gccgacaccg gcaccccgca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1020

cacgccaagc gccagaccgu cguggugcug ggcagccagg agggggcggu ccacacggcc     1080

cuggccggcg cccucgaggc cgagauggac ggggcgaagg gccggcuguu cuccgggcac     1140

cugaagugcc gccucaagau ggacaagcug cggcugaagg gcgugagcua cucccucugc     1200

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac ggucaccgug     1260

gaggugcagu acgccgggac cgacggcccc ugcaagaucc cgguccagau ggcgguggac     1320

augcagaccc ugacgcccgu ggggcgccuc aucaccgcca accccgucau caccgagagc     1380

accgagaacu ccaagaugau gcuggagcug gacccgccgu ucggcgacag cuacaucgug     1440

aucggggugg gcgacaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg     1500

aaggccuucg aggccaccgu ccgcggcgcg aagcggaugg ccgugcucgg ggacaccgcc     1560

ugggacuucg gcagcguggg cggcgucuuc aacucccugg ggaaggggau ccaccagauc     1620

uucggcgccg ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc     1680

gggacgcugc ugguguggcu cggccugaac accaagaacg gcuccaucag ccugaccugc     1740

cucgcgcugg gcggcgugau gaucuuccug uccaccgccg ucagcgccug aggacuaguu     1800

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     1860

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  10068
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10068
gggagaaagc uuaccaugac cagcgugggg aucgugggcc uccugcugac gaccgccaug       60

gcggccguga cccugcccag ccacuccacc cgcaagcugc agacgcggag ccagaccugg      120

cucgaguccc gcgaguacac caagcaccug auccgggucg agaacuggau cuuccgcaac      180

ccgggcuucg cccuggccgc ggccgccauc gccuggcucc uggggagcuc caccagccag      240

aaggugaucu accuggugau gauccuccug aucgcccccg cguacuccau ccgcugcauc      300

ggcgucagca accgggacuu cguggagggg auguccggcg gcacgugggu ggacgucgug      360

cuggagcacg ggggcugcgu gaccgucaug gcccaggaca agcccaccgu ggacaucgag      420

cucgugacca cgaccgucag caacauggcc gaggugcgca gcuacugcua cgaggccucc      480

aucagcgaca uggccuccga cagccggugc cccacccagg gggaggcgua ccuggacaag      540

caguccgaca cccaguacgu cugcaagcgc acgcuggugg accggggcug ggggaacggc      600

ugcggccucu ucgggaaggg cagccuggug accugcgcca aguucgccug cuccaagaag      660

augaccggga agagcaucca gccggagaac cuggaguacc gcaucaugcu cuccguccac      720

ggcagccagc acuccgggau gaucgugaac gacaccggcc acgagacgga cgagaaccgg      780

gccaaggugg agaucacccc caacagcccc cgcgcggagg ccacccuggg cggguucggc      840

ucccuggggc ucgacugcga gccccggacc ggccuggacu ucagcgaccu guacuaccuc      900

acgaugaaca acaagcacug gcugguccac aaggaguggu uccacgacau cccgcugccc      960

uggcacgccg gcgccgacac cgggaccccg cacuggaaca acaaggaggc ccucguggag     1020

uucaaggacg cgcacgccaa gcgccagacc guggucgugc ugggcuccca ggagggggcc     1080

gugcacacgg cccuggccgg cgcgcuggag gccgagaugg acggggccaa gggccggcuc     1140

agcuccggcc accugaagug ccgccugaag auggacaagc uccggcugaa gggggucagc     1200

uacucccugu gcaccgccgc cuucaccuuc accaagaucc ccgcggagac ccuccacggc     1260

acggugaccg uggaggucca guacgccggg accgacggcc cgugcaaggu gcccgcccag     1320

auggccgugg acaugcagac ccugacgccc gucgggcgcc ugaucaccgc gaaccccgug     1380

aucaccgaga gcaccgagaa cagcaagaug augcucgagc uggacccgcc cuucggcgac     1440

uccuacaucg ugaucggcgu cggggagaag aagaucacgc accacuggca ccggagcggc     1500

uccaccaucg ggaaggccuu cgaggccacc gugcgcggcg ccaagcggau ggccgugcug     1560

ggggacaccg cgugggacuu cggcagcguc ggcggggccc ucaacucccu gggcaagggg     1620

auccaccaga ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc     1680

cagauccuca ucggcacgcu gcugaugugg cucgggcuga acaccaagaa cggcuccauc     1740

agccugaugu gccucgcccu gggcggcgug cugaucuucc ucuccaccgc ggugagcgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10069
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10069
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gccgugaccc ugcccagcca cuccacccgc      120

aagcugcaga cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc      180

cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc      240

uggcuccugg ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc      300

gcccccgcgu acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug      360

uccggcggca cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc      420

caggacaagc ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag      480

guccgcagcu acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc      540

acccaggggg aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg      600

cuggucgacc ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc      660

ugcgccaagu ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug      720

gaguaccgca ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac      780

accggccacg agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc      840

gcggaggcca cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc      900

cuggacuuca gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag      960

gagugguucc acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac     1020

uggaacaaca aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug     1080

gugguccugg gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc     1140

gagauggacg gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug     1200

gacaagcucc ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc     1260

aagauccccg cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc     1320

gacggcccgu gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug     1380

gggcgccuga ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug     1440

cucgagcugg acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag     1500

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc     1560

cgcggcgcca agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc     1620

ggggcccuca acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1680

cuguucgggg gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc     1740

gggcugaaca ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug     1800

aucuuccucu ccaccgcggu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10070
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10070
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gccgugaccc ugcccagcca cuccacgcgc      120

aagcugcaga cccggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc      180

cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc      240

uggcuccugg ggagcuccac gagccagaag gugaucuacc uggugaugau ccuccugauc      300

gcccccgcgu acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug      360

uccggcggca ccugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc      420

caggacaagc ccaccgugga caucgagcuc gugacgacca ccgucagcaa cauggccgag      480

gugcgcagcu acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc      540

acccaggggg aggcguaccu ggacaagcag uccgacacgc aguacgucug caagcgcacc      600

cugguggacc ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc      660

ugcgccaagu ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug      720

gaguaccgca ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac      780

acgggccacg agaccgacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc      840

gcggaggcca cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggacgggc      900

cuggacuuca gcgaccugua cuaccucacc augaacaaca agcacuggcu gguccacaag      960

gagugguucc acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac     1020

uggaacaaca aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagacggug     1080

gucgugcugg gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc     1140

gagauggacg gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug     1200

gacaagcucc ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc     1260

aagauccccg cggagacgcu ccacggcacc gugaccgugg agguccagua cgccgggacc     1320

gacggcccgu gcaaggugcc cgcccagaug gccguggaca ugcagacgcu gacccccguc     1380

gggcgccuga ucaccgcgaa ccccgugauc accgagagca cggagaacag caagaugaug     1440

cucgagcugg acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag     1500

aucacccacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug     1560

cgcggcgcca agcggauggc cgugcugggg gacacggcgu gggacuucgg cagcgucggc     1620

ggggcccuca acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1680

cuguucgggg gcauguccug guucagccag auccucaucg gcacccugcu gauguggcuc     1740

gggcugaacg ccaagaacgg cuccaucagc cugaugugcc ucgcgcuggg cggcgugcug     1800

aucuuccucu ccaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10071
<211>  2020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10071
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gccgugaccc ugcccuccca cagcacccgg      120

aagcuccaga cgcgcuccca gaccuggcug gagagccgcg aguacaccaa gcaccugauc      180

aagguggaga acuggaucuu ccggaacccc ggcuucgccc ucgucgcggu ggccaucgcc      240

uggcugcugg gguccagcac cucccagaag gugaucuacc ucgucaugau ccugcugauc      300

gccccggccu acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug      360

agcggcggga cgugggucga cguggugcuc gagcacggcg gcugcgucac cgugauggcg      420

caggacaagc ccaccgugga caucgagcug gucaccacga ccguguccaa cauggccgag      480

guccgcagcu acugcuacga ggccagcauc uccgacaugg ccagcgacuc ccggugcccc      540

acccaggggg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagcgcacg      600

cucguggacc ggggcugggg gaacggcugc gggcuguucg gcaagggcuc ccuggucacg      660

ugcgcgaagu ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccuc      720

gaguaccgca ucaugcugag cgugcacggc ucccagcaca gcgggaugau cggcuacgag      780

acggacgagg accgggccaa gguggagguc accccgaacu ccccgcgcgc cgaggccacc      840

cugggcgggu ucggcagccu cgggcuggac ugcgagcccc ggaccggccu ggacuucucc      900

gaccucuacu accugacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac      960

gacauccccc ucccguggca cgcgggggcc gacaccggca ccccgcacug gaacaacaag     1020

gaggcccugg uggaguucaa ggacgcccac gccaagcgcc agaccgucgu ggugcugggc     1080

agccaggagg gggcggucca cacggcccug gccggcgccc ucgaggccga gauggacggg     1140

gcgaagggcc ggcuguucuc cgggcaccug aagugccgcc ucaagaugga caagcugcgg     1200

cugaagggcg ugagcuacuc ccucugcacc gccgccuuca ccuucaccaa ggugcccgcc     1260

gagacccugc acggcacggu caccguggag gugcaguacg ccgggaccga cggccccugc     1320

aagaucccgg uccagauggc gguggacaug cagacccuga cgcccguggg gcgccucauc     1380

accgccaacc ccgucaucac cgagagcacc gagaacucca agaugaugcu ggagcuggac     1440

ccgccguucg gcgacagcua caucgugauc ggggugggcg acaagaagau cacgcaccac     1500

uggcaccgga gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgcgaag     1560

cggauggccg ugcucgggga caccgccugg gacuucggca gcgugggcgg cgucuucaac     1620

ucccugggga aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc     1680

auguccuggu ucagccagau ccucaucggg acgcugcugg uguggcucgg ccugaacacc     1740

aagaacggcu ccaucagccu gaccugccuc gcgcugggcg gcgugaugau cuuccugucc     1800

accgccguca gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     1860

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1980

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2020


<210>  10072
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10072
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu caucaucgcc       60

gugcucaugu ccgcgcagga gagcugggcc gccgugaccc ugcccagcca cuccacccgc      120

aagcugcaga cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc      180

cgggucgaga acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc      240

uggcuccugg ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc      300

gcccccgcgu acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug      360

uccggcggca cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc      420

caggacaagc ccaccgugga caucgagcuc gugaccacga ccgucagcaa cauggccgag      480

gugcgcagcu acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc      540

acccaggggg aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacg      600

cugguggacc ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc      660

ugcgccaagu ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug      720

gaguaccgca ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac      780

accggccacg agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc      840

gcggaggcca cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc      900

cuggacuuca gcgaccugua cuaccucacg augaacaaca agcacuggcu gguccacaag      960

gagugguucc acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac     1020

uggaacaaca aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagaccgug     1080

gucgugcugg gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc     1140

gagauggacg gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug     1200

gacaagcucc ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc     1260

aagauccccg cggagacccu ccacggcacg gugaccgugg agguccagua cgccgggacc     1320

gacggcccgu gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacgcccguc     1380

gggcgccuga ucaccgcgaa ccccgugauc accgagagca ccgagaacag caagaugaug     1440

cucgagcugg acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag     1500

aucacgcacc acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug     1560

cgcggcgcca agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgucggc     1620

ggggcccuca acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc     1680

cuguucgggg gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc     1740

gggcugaaca ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcgugcug     1800

aucuuccucu ccaccgcggu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10073
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10073
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      120

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      180

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      240

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      300

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      360

aucguccugg agcacggggg cugcgugacc gugauggccc aggacaagcc caccgucgac      420

aucgagcucg ugaccacgac cgugagcaac auggccgagg uccgcagcua cugcuacgag      480

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      540

gacaagcagu ccgacaccca guacgugugc aagcgcacgc uggucgaccg gggcuggggg      600

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      660

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      720

gugcacggca gccagcacuc cgggaugauc gucaacgaca ccggccacga gacggacgag      780

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      840

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      900

uaccucacga ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccg      960

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1020

gucgaguuca aggacgcgca cgccaagcgc cagaccgugg ugguccuggg cucccaggag     1080

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1140

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1200

gugagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1260

cacggcacgg ucaccgugga ggugcaguac gccgggaccg acggcccgug caaggucccc     1320

gcccagaugg ccguggacau gcagacccug acgcccgugg ggcgccugau caccgcgaac     1380

cccgucauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1440

ggcgacuccu acaucgugau cggcgugggg gagaagaaga ucacgcacca cuggcaccgg     1500

agcggcucca ccaucgggaa ggccuucgag gccaccgucc gcggcgccaa gcggauggcc     1560

gugcuggggg acaccgcgug ggacuucggc agcgugggcg gggcccucaa cucccugggc     1620

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1680

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     1740

uccaucagcc ugaugugccu cgcccugggc ggcguccuga ucuuccucuc caccgcggug     1800

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10074
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10074
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ccgugacccu gcccagccac uccacgcgca agcugcagac ccggagccag      120

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      180

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacg      240

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      300

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac cuggguggac      360

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      420

aucgagcucg ugacgaccac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      480

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      540

gacaagcagu ccgacacgca guacgucugc aagcgcaccc ugguggaccg gggcuggggg      600

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      660

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      720

guccacggca gccagcacuc cgggaugauc gugaacgaca cgggccacga gaccgacgag      780

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      840

uucggcuccc uggggcucga cugcgagccc cggacgggcc uggacuucag cgaccuguac      900

uaccucacca ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg      960

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1020

guggaguuca aggacgcgca cgccaagcgc cagacggugg ucgugcuggg cucccaggag     1080

ggggccgugc acaccgcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1140

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1200

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacgcuc     1260

cacggcaccg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1320

gcccagaugg ccguggacau gcagacgcug acccccgucg ggcgccugau caccgcgaac     1380

cccgugauca ccgagagcac ggagaacagc aagaugaugc ucgagcugga cccgcccuuc     1440

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacccacca cuggcaccgg     1500

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1560

gugcuggggg acacggcgug ggacuucggc agcgucggcg gggcccucaa cucccugggc     1620

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1680

uucagccaga uccucaucgg cacccugcug auguggcucg ggcugaacgc caagaacggc     1740

uccaucagcc ugaugugccu cgcgcugggc ggcgugcuga ucuuccucuc caccgccgug     1800

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10075
<211>  1999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10075
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ccgugacccu gcccucccac agcacccgga agcuccagac gcgcucccag      120

accuggcugg agagccgcga guacaccaag caccugauca agguggagaa cuggaucuuc      180

cggaaccccg gcuucgcccu cgucgcggug gccaucgccu ggcugcuggg guccagcacc      240

ucccagaagg ugaucuaccu cgucaugauc cugcugaucg ccccggccua cagcauccgc      300

ugcaucggcg uguccaaccg ggacuucgug gaggggauga gcggcgggac gugggucgac      360

guggugcucg agcacggcgg cugcgucacc gugauggcgc aggacaagcc caccguggac      420

aucgagcugg ucaccacgac cguguccaac auggccgagg uccgcagcua cugcuacgag      480

gccagcaucu ccgacauggc cagcgacucc cggugcccca cccaggggga ggccuaccug      540

gacaagcaga gcgacaccca guacgugugc aagcgcacgc ucguggaccg gggcuggggg      600

aacggcugcg ggcuguucgg caagggcucc cuggucacgu gcgcgaaguu cacgugcagc      660

aagaagauga ccgggaaguc cauccagccc gagaaccucg aguaccgcau caugcugagc      720

gugcacggcu cccagcacag cgggaugauc ggcuacgaga cggacgagga ccgggccaag      780

guggagguca ccccgaacuc cccgcgcgcc gaggccaccc ugggcggguu cggcagccuc      840

gggcuggacu gcgagccccg gaccggccug gacuucuccg accucuacua ccugacgaug      900

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu cccguggcac      960

gcgggggccg acaccggcac cccgcacugg aacaacaagg aggcccuggu ggaguucaag     1020

gacgcccacg ccaagcgcca gaccgucgug gugcugggca gccaggaggg ggcgguccac     1080

acggcccugg ccggcgcccu cgaggccgag auggacgggg cgaagggccg gcuguucucc     1140

gggcaccuga agugccgccu caagauggac aagcugcggc ugaagggcgu gagcuacucc     1200

cucugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcacgguc     1260

accguggagg ugcaguacgc cgggaccgac ggccccugca agaucccggu ccagauggcg     1320

guggacaugc agacccugac gcccgugggg cgccucauca ccgccaaccc cgucaucacc     1380

gagagcaccg agaacuccaa gaugaugcug gagcuggacc cgccguucgg cgacagcuac     1440

aucgugaucg gggugggcga caagaagauc acgcaccacu ggcaccggag cggcuccacc     1500

aucgggaagg ccuucgaggc caccguccgc ggcgcgaagc ggauggccgu gcucggggac     1560

accgccuggg acuucggcag cgugggcggc gucuucaacu cccuggggaa ggggauccac     1620

cagaucuucg gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc     1680

cucaucggga cgcugcuggu guggcucggc cugaacacca agaacggcuc caucagccug     1740

accugccucg cgcugggcgg cgugaugauc uuccugucca ccgccgucag cgccugagga     1800

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     1860

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     1980

uuucagagcc accagaauu                                                  1999


<210>  10076
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10076
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ccgugacccu gcccagccac uccacccgca agcugcagac gcggagccag      120

accuggcucg agucccgcga guacaccaag caccugaucc gggucgagaa cuggaucuuc      180

cgcaacccgg gcuucgcccu ggccgcggcc gccaucgccu ggcuccuggg gagcuccacc      240

agccagaagg ugaucuaccu ggugaugauc cuccugaucg cccccgcgua cuccauccgc      300

ugcaucggcg ucagcaaccg ggacuucgug gaggggaugu ccggcggcac guggguggac      360

gucgugcugg agcacggggg cugcgugacc gucauggccc aggacaagcc caccguggac      420

aucgagcucg ugaccacgac cgucagcaac auggccgagg ugcgcagcua cugcuacgag      480

gccuccauca gcgacauggc cuccgacagc cggugcccca cccaggggga ggcguaccug      540

gacaagcagu ccgacaccca guacgucugc aagcgcacgc ugguggaccg gggcuggggg      600

aacggcugcg gccucuucgg gaagggcagc cuggugaccu gcgccaaguu cgccugcucc      660

aagaagauga ccgggaagag cauccagccg gagaaccugg aguaccgcau caugcucucc      720

guccacggca gccagcacuc cgggaugauc gugaacgaca ccggccacga gacggacgag      780

aaccgggcca agguggagau cacccccaac agcccccgcg cggaggccac ccugggcggg      840

uucggcuccc uggggcucga cugcgagccc cggaccggcc uggacuucag cgaccuguac      900

uaccucacga ugaacaacaa gcacuggcug guccacaagg agugguucca cgacaucccg      960

cugcccuggc acgccggcgc cgacaccggg accccgcacu ggaacaacaa ggaggcccuc     1020

guggaguuca aggacgcgca cgccaagcgc cagaccgugg ucgugcuggg cucccaggag     1080

ggggccgugc acacggcccu ggccggcgcg cuggaggccg agauggacgg ggccaagggc     1140

cggcucagcu ccggccaccu gaagugccgc cugaagaugg acaagcuccg gcugaagggg     1200

gucagcuacu cccugugcac cgccgccuuc accuucacca agauccccgc ggagacccuc     1260

cacggcacgg ugaccgugga gguccaguac gccgggaccg acggcccgug caaggugccc     1320

gcccagaugg ccguggacau gcagacccug acgcccgucg ggcgccugau caccgcgaac     1380

cccgugauca ccgagagcac cgagaacagc aagaugaugc ucgagcugga cccgcccuuc     1440

ggcgacuccu acaucgugau cggcgucggg gagaagaaga ucacgcacca cuggcaccgg     1500

agcggcucca ccaucgggaa ggccuucgag gccaccgugc gcggcgccaa gcggauggcc     1560

gugcuggggg acaccgcgug ggacuucggc agcgucggcg gggcccucaa cucccugggc     1620

aaggggaucc accagaucuu cggcgccgcc uucaagagcc uguucggggg cauguccugg     1680

uucagccaga uccucaucgg cacgcugcug auguggcucg ggcugaacac caagaacggc     1740

uccaucagcc ugaugugccu cgcccugggc ggcgugcuga ucuuccucuc caccgcggug     1800

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10077
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10077
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccgcc gugacccugc ccagccacuc cacccgcaag      120

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      180

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      240

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      300

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      360

ggcggcacgu ggguggacau cguccuggag cacgggggcu gcgugaccgu gauggcccag      420

gacaagccca ccgucgacau cgagcucgug accacgaccg ugagcaacau ggccgagguc      480

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      540

cagggggagg cguaccugga caagcagucc gacacccagu acgugugcaa gcgcacgcug      600

gucgaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      660

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      720

uaccgcauca ugcucuccgu gcacggcagc cagcacuccg ggaugaucgu caacgacacc      780

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      840

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      900

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu gcacaaggag      960

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1020

aacaacaagg aggcccucgu cgaguucaag gacgcgcacg ccaagcgcca gaccguggug     1080

guccugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1140

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1200

aagcuccggc ugaagggggu gagcuacucc cugugcaccg ccgccuucac cuucaccaag     1260

auccccgcgg agacccucca cggcacgguc accguggagg ugcaguacgc cgggaccgac     1320

ggcccgugca agguccccgc ccagauggcc guggacaugc agacccugac gcccgugggg     1380

cgccugauca ccgcgaaccc cgucaucacc gagagcaccg agaacagcaa gaugaugcuc     1440

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcguggggga gaagaagauc     1500

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccguccgc     1560

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgugggcggg     1620

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1680

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     1740

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cguccugauc     1800

uuccucucca ccgcggugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10078
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10078
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccgcc gugacccugc ccagccacuc cacgcgcaag      120

cugcagaccc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      180

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      240

cuccugggga gcuccacgag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      300

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      360

ggcggcaccu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      420

gacaagccca ccguggacau cgagcucgug acgaccaccg ucagcaacau ggccgaggug      480

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      540

cagggggagg cguaccugga caagcagucc gacacgcagu acgucugcaa gcgcacccug      600

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      660

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      720

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacg      780

ggccacgaga ccgacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      840

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gacgggccug      900

gacuucagcg accuguacua ccucaccaug aacaacaagc acuggcuggu ccacaaggag      960

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1020

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gacggugguc     1080

gugcugggcu cccaggaggg ggccgugcac accgcccugg ccggcgcgcu ggaggccgag     1140

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1200

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1260

auccccgcgg agacgcucca cggcaccgug accguggagg uccaguacgc cgggaccgac     1320

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacgcugac ccccgucggg     1380

cgccugauca ccgcgaaccc cgugaucacc gagagcacgg agaacagcaa gaugaugcuc     1440

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1500

acccaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1560

ggcgccaagc ggauggccgu gcugggggac acggcguggg acuucggcag cgucggcggg     1620

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1680

uucgggggca uguccugguu cagccagauc cucaucggca cccugcugau guggcucggg     1740

cugaacgcca agaacggcuc caucagccug augugccucg cgcugggcgg cgugcugauc     1800

uuccucucca ccgccgugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10079
<211>  2017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10079
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccgcc gugacccugc ccucccacag cacccggaag      120

cuccagacgc gcucccagac cuggcuggag agccgcgagu acaccaagca ccugaucaag      180

guggagaacu ggaucuuccg gaaccccggc uucgcccucg ucgcgguggc caucgccugg      240

cugcuggggu ccagcaccuc ccagaaggug aucuaccucg ucaugauccu gcugaucgcc      300

ccggccuaca gcauccgcug caucggcgug uccaaccggg acuucgugga ggggaugagc      360

ggcgggacgu gggucgacgu ggugcucgag cacggcggcu gcgucaccgu gauggcgcag      420

gacaagccca ccguggacau cgagcugguc accacgaccg uguccaacau ggccgagguc      480

cgcagcuacu gcuacgaggc cagcaucucc gacauggcca gcgacucccg gugccccacc      540

cagggggagg ccuaccugga caagcagagc gacacccagu acgugugcaa gcgcacgcuc      600

guggaccggg gcugggggaa cggcugcggg cuguucggca agggcucccu ggucacgugc      660

gcgaaguuca cgugcagcaa gaagaugacc gggaagucca uccagcccga gaaccucgag      720

uaccgcauca ugcugagcgu gcacggcucc cagcacagcg ggaugaucgg cuacgagacg      780

gacgaggacc gggccaaggu ggaggucacc ccgaacuccc cgcgcgccga ggccacccug      840

ggcggguucg gcagccucgg gcuggacugc gagccccgga ccggccugga cuucuccgac      900

cucuacuacc ugacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      960

aucccccucc cguggcacgc gggggccgac accggcaccc cgcacuggaa caacaaggag     1020

gcccuggugg aguucaagga cgcccacgcc aagcgccaga ccgucguggu gcugggcagc     1080

caggaggggg cgguccacac ggcccuggcc ggcgcccucg aggccgagau ggacggggcg     1140

aagggccggc uguucuccgg gcaccugaag ugccgccuca agauggacaa gcugcggcug     1200

aagggcguga gcuacucccu cugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1260

acccugcacg gcacggucac cguggaggug caguacgccg ggaccgacgg ccccugcaag     1320

aucccggucc agauggcggu ggacaugcag acccugacgc ccguggggcg ccucaucacc     1380

gccaaccccg ucaucaccga gagcaccgag aacuccaaga ugaugcugga gcuggacccg     1440

ccguucggcg acagcuacau cgugaucggg gugggcgaca agaagaucac gcaccacugg     1500

caccggagcg gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgcgaagcgg     1560

auggccgugc ucggggacac cgccugggac uucggcagcg ugggcggcgu cuucaacucc     1620

cuggggaagg ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug     1680

uccugguuca gccagauccu caucgggacg cugcuggugu ggcucggccu gaacaccaag     1740

aacggcucca ucagccugac cugccucgcg cugggcggcg ugaugaucuu ccuguccacc     1800

gccgucagcg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     1860

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1980

ccccccccca aaggcucuuu ucagagccac cagaauu                              2017


<210>  10080
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10080
gggagaaagc uuaccauggg caagcgcagc gccgggucca ucauguggcu ggccagccuc       60

gcggugguca ucgccugcgc cggcgccgcc gugacccugc ccagccacuc cacccgcaag      120

cugcagacgc ggagccagac cuggcucgag ucccgcgagu acaccaagca ccugauccgg      180

gucgagaacu ggaucuuccg caacccgggc uucgcccugg ccgcggccgc caucgccugg      240

cuccugggga gcuccaccag ccagaaggug aucuaccugg ugaugauccu ccugaucgcc      300

cccgcguacu ccauccgcug caucggcguc agcaaccggg acuucgugga ggggaugucc      360

ggcggcacgu ggguggacgu cgugcuggag cacgggggcu gcgugaccgu cauggcccag      420

gacaagccca ccguggacau cgagcucgug accacgaccg ucagcaacau ggccgaggug      480

cgcagcuacu gcuacgaggc cuccaucagc gacauggccu ccgacagccg gugccccacc      540

cagggggagg cguaccugga caagcagucc gacacccagu acgucugcaa gcgcacgcug      600

guggaccggg gcugggggaa cggcugcggc cucuucggga agggcagccu ggugaccugc      660

gccaaguucg ccugcuccaa gaagaugacc gggaagagca uccagccgga gaaccuggag      720

uaccgcauca ugcucuccgu ccacggcagc cagcacuccg ggaugaucgu gaacgacacc      780

ggccacgaga cggacgagaa ccgggccaag guggagauca cccccaacag cccccgcgcg      840

gaggccaccc ugggcggguu cggcucccug gggcucgacu gcgagccccg gaccggccug      900

gacuucagcg accuguacua ccucacgaug aacaacaagc acuggcuggu ccacaaggag      960

ugguuccacg acaucccgcu gcccuggcac gccggcgccg acaccgggac cccgcacugg     1020

aacaacaagg aggcccucgu ggaguucaag gacgcgcacg ccaagcgcca gaccgugguc     1080

gugcugggcu cccaggaggg ggccgugcac acggcccugg ccggcgcgcu ggaggccgag     1140

auggacgggg ccaagggccg gcucagcucc ggccaccuga agugccgccu gaagauggac     1200

aagcuccggc ugaagggggu cagcuacucc cugugcaccg ccgccuucac cuucaccaag     1260

auccccgcgg agacccucca cggcacggug accguggagg uccaguacgc cgggaccgac     1320

ggcccgugca aggugcccgc ccagauggcc guggacaugc agacccugac gcccgucggg     1380

cgccugauca ccgcgaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcuc     1440

gagcuggacc cgcccuucgg cgacuccuac aucgugaucg gcgucgggga gaagaagauc     1500

acgcaccacu ggcaccggag cggcuccacc aucgggaagg ccuucgaggc caccgugcgc     1560

ggcgccaagc ggauggccgu gcugggggac accgcguggg acuucggcag cgucggcggg     1620

gcccucaacu cccugggcaa ggggauccac cagaucuucg gcgccgccuu caagagccug     1680

uucgggggca uguccugguu cagccagauc cucaucggca cgcugcugau guggcucggg     1740

cugaacacca agaacggcuc caucagccug augugccucg cccugggcgg cgugcugauc     1800

uuccucucca ccgcggugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10081
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10081
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10082
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10082
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaccugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acgccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10083
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10083
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc aucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcuccgacgc cggcaaggcc aucuccuucg ccaccacccu cggcgucaac      180

aagugccacg uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc aucaaggucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgucgc cgucgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucaccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucggcuac gagaccgacg aggaccgcgc caaggucgag     1080

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaagaucc ccguccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1800

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1860

ugggacuucg gcuccgucgg cggcgucuuc aacucccucg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1980

ggcacccucc ucgucuggcu cggccucaac accaagaacg gcuccaucuc ccucaccugc     2040

cucgcccucg gcggcgucau gaucuuccuc uccaccgccg ucuccgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10084
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10084
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1860

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1980

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     2040

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10085
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10085
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10086
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10086
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa cgccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10087
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10087
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacucca ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1800

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1860

ggcgucuuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1920

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cgucuggcuc     1980

ggccucaaca ccaagaacgg cuccaucucc cucaccugcc ucgcccucgg cggcgucaug     2040

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10088
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10088
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccaccaucgg caaggccuuc     1800

gaggccaccg uccgcggcgc caagcgcaug gccguccucg gcgacaccgc cugggacuuc     1860

ggcuccgucg gcggcgcccu caacucccuc ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaagu cccucuucgg cggcaugucc ugguucuccc agauccucau cggcacccuc     1980

cucauguggc ucggccucaa caccaagaac ggcuccaucu cccucaugug ccucgcccuc     2040

ggcggcgucc ucaucuuccu cuccaccgcc gucuccgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10089
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10089
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10090
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10090
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac accugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga cgucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaacg ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10091
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10091
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccauc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgcu ccgacgccgg caaggccauc uccuucgcca ccacccucgg cgucaacaag      180

ugccacgucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc aaggucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga cgucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucaccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cggcuacgag accgacgagg accgcgccaa ggucgagguc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuucuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gguccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagauccccg uccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg acaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag     1800

gccuucgagg ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg     1860

gacuucggcu ccgucggcgg cgucuucaac ucccucggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc     1980

acccuccucg ucuggcucgg ccucaacacc aagaacggcu ccaucucccu caccugccuc     2040

gcccucggcg gcgucaugau cuuccucucc accgccgucu ccgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10092
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10092
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga cgucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

accaucggca aggccuucga ggccaccguc cgcggcgcca agcgcauggc cguccucggc     1860

gacaccgccu gggacuucgg cuccgucggc ggcgcccuca acucccucgg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagucc cucuucggcg gcauguccug guucucccag     1980

auccucaucg gcacccuccu cauguggcuc ggccucaaca ccaagaacgg cuccaucucc     2040

cucaugugcc ucgcccucgg cggcguccuc aucuuccucu ccaccgccgu cuccgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10093
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10093
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccacccucg gcaaggccuu cuccaccacc cucaagggcg cccagcgccu cgccgcccuc     1860

ggcgacaccg ccugggacuu cggcuccauc ggcggcgucu ucaacuccau cggccgcgcc     1920

guccaccagg ucuucggcgg cgccuuccgc acccucuucg gcggcauguc cuggaucacc     1980

cagggccuca ugggcgcccu ccuccucugg augggcguca acgcccgcga ccgcuccauc     2040

gcccucgccu uccucgccac cggcggcguc cucgucuucc ucgccaccaa cguccacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10094
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10094
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaccugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccacccucg gcaaggccuu cuccaccacc cucaagggcg cccagcgccu cgccgcccuc     1860

ggcgacaccg ccugggacuu cggcuccauc ggcggcgucu ucaacuccau cggccgcgcc     1920

guccaccagg ucuucggcgg cgccuuccgc acccucuucg gcggcauguc cuggaucacc     1980

cagggccuca ugggcgcccu ccuccucugg augggcguca acgcccgcga ccgcuccauc     2040

gcccucgccu uccucgccac cggcggcguc cucgucuucc ucgccaccaa cguccacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10095
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10095
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc aucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcuccgacgc cggcaaggcc aucuccuucg ccaccacccu cggcgucaac      180

aagugccacg uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc aucaaggucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgucgc cgucgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucaccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucggcuac gagaccgacg aggaccgcgc caaggucgag     1080

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc     1140

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac     1200

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1320

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1380

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac     1440

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1500

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1560

gagguccagu acgccggcac cgacggcccc ugcaagaucc ccguccagau ggccgucgac     1620

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1680

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1740

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc cacccucggc     1800

aaggccuucu ccaccacccu caagggcgcc cagcgccucg ccgcccucgg cgacaccgcc     1860

ugggacuucg gcuccaucgg cggcgucuuc aacuccaucg gccgcgccgu ccaccagguc     1920

uucggcggcg ccuuccgcac ccucuucggc ggcauguccu ggaucaccca gggccucaug     1980

ggcgcccucc uccucuggau gggcgucaac gcccgcgacc gcuccaucgc ccucgccuuc     2040

cucgccaccg gcggcguccu cgucuuccuc gccaccaacg uccacgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10096
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10096
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gucacccgcc gcggcuccgc cuacuacaug      120

uaccucgacc gcaacgacgc cggcgaggcc aucuccuucc ccaccacccu cggcaugaac      180

aagugcuaca uccagaucau ggaccucggc cacaugugcg acgccaccau guccuacgag      240

ugccccaugc ucgacgaggg cgucgagccc gacgacgucg acugcuggug caacaccacc      300

uccaccuggg ucgucuacgg caccugccac cacaagaagg gcgaggcccg ccgcucccgc      360

cgcgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      420

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      480

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      540

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      600

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      660

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      720

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      780

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      840

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      900

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      960

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac     1020

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc     1080

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc     1140

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc     1200

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1320

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1380

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1440

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1500

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1560

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1620

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1680

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1740

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1800

uccacccucg gcaaggccuu cuccaccacc cucaagggcg cccagcgccu cgccgcccuc     1860

ggcgacaccg ccugggacuu cggcuccauc ggcggcgucu ucaacuccau cggccgcgcc     1920

guccaccagg ucuucggcgg cgccuuccgc acccucuucg gcggcauguc cuggaucacc     1980

cagggccuca ugggcgcccu ccuccucugg augggcguca acgcccgcga ccgcuccauc     2040

gcccucgccu uccucgccac cggcggcguc cucgucuucc ucgccaccaa cguccacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10097
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10097
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacaucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10098
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10098
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaccugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10099
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10099
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacucca ucacccgccg cggcuccgcc uacuacaugu accucgaccg cuccgacgcc      120

ggcaaggcca ucuccuucgc caccacccuc ggcgucaaca agugccacgu ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca ucaaggucga gaacuggauc uuccgcaacc ccggcuucgc ccucgucgcc      480

gucgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucaccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucggcuacg agaccgacga ggaccgcgcc aaggucgagg ucacccccaa cuccccccgc     1080

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc     1140

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag     1200

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1320

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1380

gagauggacg gcgccaaggg ccgccucuuc uccggccacc ucaagugccg ccucaagaug     1440

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1500

aagguccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1560

gacggccccu gcaagauccc cguccagaug gccgucgaca ugcagacccu cacccccguc     1620

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1680

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgacaagaag     1740

aucacccacc acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc     1800

aagggcgccc agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc     1860

ggcgucuuca acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc     1920

cucuucggcg gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug     1980

ggcgucaacg cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc     2040

gucuuccucg ccaccaacgu ccacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10100
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10100
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ucacccgccg cggcuccgcc uacuacaugu accucgaccg caacgacgcc      120

ggcgaggcca ucuccuuccc caccacccuc ggcaugaaca agugcuacau ccagaucaug      180

gaccucggcc acaugugcga cgccaccaug uccuacgagu gccccaugcu cgacgagggc      240

gucgagcccg acgacgucga cugcuggugc aacaccaccu ccaccugggu cgucuacggc      300

accugccacc acaagaaggg cgaggcccgc cgcucccgcc gcgccgucac ccuccccucc      360

cacuccaccc gcaagcucca gacccgcucc cagaccuggc ucgagucccg cgaguacacc      420

aagcaccuca uccgcgucga gaacuggauc uuccgcaacc ccggcuucgc ccucgccgcc      480

gccgccaucg ccuggcuccu cggcuccucc accucccaga aggucaucua ccucgucaug      540

auccuccuca ucgcccccgc cuacuccauc cgcugcaucg gcgucuccaa ccgcgacuuc      600

gucgagggca uguccggcgg caccuggguc gacgucgucc ucgagcacgg cggcugcguc      660

accgucaugg cccaggacaa gcccaccguc gacaucgagc ucgucaccac caccgucucc      720

aacauggccg agguccgcuc cuacugcuac gaggccucca ucuccgacau ggccuccgac      780

ucccgcugcc ccacccaggg cgaggccuac cucgacaagc aguccgacac ccaguacguc      840

ugcaagcgca cccucgucga ccgcggcugg ggcaacggcu gcggccucuu cggcaagggc      900

ucccucguca ccugcgccaa guucgccugc uccaagaaga ugaccggcaa guccauccag      960

cccgagaacc ucgaguaccg caucaugcuc uccguccacg gcucccagca cuccggcaug     1020

aucgucaacg acaccggcca cgagaccgac gagaaccgcg ccaaggucga gaucaccccc     1080

aacucccccc gcgccgaggc cacccucggc ggcuucggcu cccucggccu cgacugcgag     1140

ccccgcaccg gccucgacuu cuccgaccuc uacuaccuca ccaugaacaa caagcacugg     1200

cucguccaca aggagugguu ccacgacauc ccccuccccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cucgucgagu ucaaggacgc ccacgccaag     1320

cgccagaccg ucgucguccu cggcucccag gagggcgccg uccacaccgc ccucgccggc     1380

gcccucgagg ccgagaugga cggcgccaag ggccgccucu ccuccggcca ccucaagugc     1440

cgccucaaga uggacaagcu ccgccucaag ggcgucuccu acucccucug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cuccacggca ccgucaccgu cgagguccag     1560

uacgccggca ccgacggccc cugcaagguc cccgcccaga uggccgucga caugcagacc     1620

cucacccccg ucggccgccu caucaccgcc aaccccguca ucaccgaguc caccgagaac     1680

uccaagauga ugcucgagcu cgaccccccc uucggcgacu ccuacaucgu caucggcguc     1740

ggcgagaaga agaucaccca ccacuggcac cgcuccggcu ccacccucgg caaggccuuc     1800

uccaccaccc ucaagggcgc ccagcgccuc gccgcccucg gcgacaccgc cugggacuuc     1860

ggcuccaucg gcggcgucuu caacuccauc ggccgcgccg uccaccaggu cuucggcggc     1920

gccuuccgca cccucuucgg cggcaugucc uggaucaccc agggccucau gggcgcccuc     1980

cuccucugga ugggcgucaa cgcccgcgac cgcuccaucg cccucgccuu ccucgccacc     2040

ggcggcgucc ucgucuuccu cgccaccaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10101
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10101
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga caucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

acccucggca aggccuucuc caccacccuc aagggcgccc agcgccucgc cgcccucggc     1860

gacaccgccu gggacuucgg cuccaucggc ggcgucuuca acuccaucgg ccgcgccguc     1920

caccaggucu ucggcggcgc cuuccgcacc cucuucggcg gcauguccug gaucacccag     1980

ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg cccgcgaccg cuccaucgcc     2040

cucgccuucc ucgccaccgg cggcguccuc gucuuccucg ccaccaacgu ccacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10102
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10102
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac accugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga cgucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

acccucggca aggccuucuc caccacccuc aagggcgccc agcgccucgc cgcccucggc     1860

gacaccgccu gggacuucgg cuccaucggc ggcgucuuca acuccaucgg ccgcgccguc     1920

caccaggucu ucggcggcgc cuuccgcacc cucuucggcg gcauguccug gaucacccag     1980

ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg cccgcgaccg cuccaucgcc     2040

cucgccuucc ucgccaccgg cggcguccuc gucuuccucg ccaccaacgu ccacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10103
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10103
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccauc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgcu ccgacgccgg caaggccauc uccuucgcca ccacccucgg cgucaacaag      180

ugccacgucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc aaggucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga cgucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucaccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cggcuacgag accgacgagg accgcgccaa ggucgagguc     1080

acccccaacu ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac     1140

ugcgagcccc gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag     1200

cacuggcucg uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac     1320

gccaagcgcc agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc     1380

gccggcgccc ucgaggccga gauggacggc gccaagggcc gccucuucuc cggccaccuc     1440

aagugccgcc ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc     1500

gccgccuuca ccuucaccaa gguccccgcc gagacccucc acggcaccgu caccgucgag     1560

guccaguacg ccggcaccga cggccccugc aagauccccg uccagauggc cgucgacaug     1620

cagacccuca cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc     1680

gagaacucca agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc     1740

ggcgucggcg acaagaagau cacccaccac uggcaccgcu ccggcuccac ccucggcaag     1800

gccuucucca ccacccucaa gggcgcccag cgccucgccg cccucggcga caccgccugg     1860

gacuucggcu ccaucggcgg cgucuucaac uccaucggcc gcgccgucca ccaggucuuc     1920

ggcggcgccu uccgcacccu cuucggcggc auguccugga ucacccaggg ccucaugggc     1980

gcccuccucc ucuggauggg cgucaacgcc cgcgaccgcu ccaucgcccu cgccuuccuc     2040

gccaccggcg gcguccucgu cuuccucgcc accaacgucc acgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10104
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10104
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccguc acccgccgcg gcuccgccua cuacauguac      120

cucgaccgca acgacgccgg cgaggccauc uccuucccca ccacccucgg caugaacaag      180

ugcuacaucc agaucaugga ccucggccac augugcgacg ccaccauguc cuacgagugc      240

cccaugcucg acgagggcgu cgagcccgac gacgucgacu gcuggugcaa caccaccucc      300

accugggucg ucuacggcac cugccaccac aagaagggcg aggcccgccg cucccgccgc      360

gccgucaccc uccccuccca cuccacccgc aagcuccaga cccgcuccca gaccuggcuc      420

gagucccgcg aguacaccaa gcaccucauc cgcgucgaga acuggaucuu ccgcaacccc      480

ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg gcuccuccac cucccagaag      540

gucaucuacc ucgucaugau ccuccucauc gcccccgccu acuccauccg cugcaucggc      600

gucuccaacc gcgacuucgu cgagggcaug uccggcggca ccugggucga cgucguccuc      660

gagcacggcg gcugcgucac cgucauggcc caggacaagc ccaccgucga caucgagcuc      720

gucaccacca ccgucuccaa cauggccgag guccgcuccu acugcuacga ggccuccauc      780

uccgacaugg ccuccgacuc ccgcugcccc acccagggcg aggccuaccu cgacaagcag      840

uccgacaccc aguacgucug caagcgcacc cucgucgacc gcggcugggg caacggcugc      900

ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu ucgccugcuc caagaagaug      960

accggcaagu ccauccagcc cgagaaccuc gaguaccgca ucaugcucuc cguccacggc     1020

ucccagcacu ccggcaugau cgucaacgac accggccacg agaccgacga gaaccgcgcc     1080

aaggucgaga ucacccccaa cuccccccgc gccgaggcca cccucggcgg cuucggcucc     1140

cucggccucg acugcgagcc ccgcaccggc cucgacuucu ccgaccucua cuaccucacc     1200

augaacaaca agcacuggcu cguccacaag gagugguucc acgacauccc ccuccccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu cgucgaguuc     1320

aaggacgccc acgccaagcg ccagaccguc gucguccucg gcucccagga gggcgccguc     1380

cacaccgccc ucgccggcgc ccucgaggcc gagauggacg gcgccaaggg ccgccucucc     1440

uccggccacc ucaagugccg ccucaagaug gacaagcucc gccucaaggg cgucuccuac     1500

ucccucugca ccgccgccuu caccuucacc aagauccccg ccgagacccu ccacggcacc     1560

gucaccgucg agguccagua cgccggcacc gacggccccu gcaagguccc cgcccagaug     1620

gccgucgaca ugcagacccu cacccccguc ggccgccuca ucaccgccaa ccccgucauc     1680

accgagucca ccgagaacuc caagaugaug cucgagcucg acccccccuu cggcgacucc     1740

uacaucguca ucggcgucgg cgagaagaag aucacccacc acuggcaccg cuccggcucc     1800

acccucggca aggccuucuc caccacccuc aagggcgccc agcgccucgc cgcccucggc     1860

gacaccgccu gggacuucgg cuccaucggc ggcgucuuca acuccaucgg ccgcgccguc     1920

caccaggucu ucggcggcgc cuuccgcacc cucuucggcg gcauguccug gaucacccag     1980

ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg cccgcgaccg cuccaucgcc     2040

cucgccuucc ucgccaccgg cggcguccuc gucuuccucg ccaccaacgu ccacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10105
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10105
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      120

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      180

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      240

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      300

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacaucguc      360

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      420

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      480

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      540

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      600

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      660

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac      720

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc      780

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc      840

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc      900

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc      960

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1020

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1080

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1140

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1200

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1260

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1320

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1380

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1440

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1500

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1560

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1620

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1680

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     1740

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10106
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10106
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      120

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      180

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      240

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      300

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      360

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      420

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      480

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      540

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      600

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      660

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac      720

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc      780

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc      840

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc      900

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc      960

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1020

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1080

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1140

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1200

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1260

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1320

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1380

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1440

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1500

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1560

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1620

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1680

cagauccuca ucggcacccu ccucaugugg cucggccuca acgccaagaa cggcuccauc     1740

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10107
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10107
gggagaaagc uuaccaugac cuccaucggc aucaucggcc uccuccucac caccgccaug       60

gccgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      120

cucgaguccc gcgaguacac caagcaccuc aucaaggucg agaacuggau cuuccgcaac      180

cccggcuucg cccucgucgc cgucgccauc gccuggcucc ucggcuccuc caccucccag      240

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      300

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      360

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      420

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      480

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      540

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      600

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucaccug cuccaagaag      660

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac      720

ggcucccagc acuccggcau gaucggcuac gagaccgacg aggaccgcgc caaggucgag      780

gucaccccca acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc      840

gacugcgagc cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac      900

aagcacuggc ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc      960

gccgacaccg gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc     1020

cacgccaagc gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc     1080

cucgccggcg cccucgaggc cgagauggac ggcgccaagg gccgccucuu cuccggccac     1140

cucaagugcc gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc     1200

accgccgccu ucaccuucac caaggucccc gccgagaccc uccacggcac cgucaccguc     1260

gagguccagu acgccggcac cgacggcccc ugcaagaucc ccguccagau ggccgucgac     1320

augcagaccc ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc     1380

accgagaacu ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc     1440

aucggcgucg gcgacaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc     1500

aaggccuucg aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc     1560

ugggacuucg gcuccgucgg cggcgucuuc aacucccucg gcaagggcau ccaccagauc     1620

uucggcgccg ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc     1680

ggcacccucc ucgucuggcu cggccucaac accaagaacg gcuccaucuc ccucaccugc     1740

cucgcccucg gcggcgucau gaucuuccuc uccaccgccg ucuccgccug aggacuaguu     1800

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     1860

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  10108
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10108
gggagaaagc uuaccaugac cuccgucggc aucgucggcc uccuccucac caccgccaug       60

gccgccguca cccuccccuc ccacuccacc cgcaagcucc agacccgcuc ccagaccugg      120

cucgaguccc gcgaguacac caagcaccuc auccgcgucg agaacuggau cuuccgcaac      180

cccggcuucg cccucgccgc cgccgccauc gccuggcucc ucggcuccuc caccucccag      240

aaggucaucu accucgucau gauccuccuc aucgcccccg ccuacuccau ccgcugcauc      300

ggcgucucca accgcgacuu cgucgagggc auguccggcg gcaccugggu cgacgucguc      360

cucgagcacg gcggcugcgu caccgucaug gcccaggaca agcccaccgu cgacaucgag      420

cucgucacca ccaccgucuc caacauggcc gagguccgcu ccuacugcua cgaggccucc      480

aucuccgaca uggccuccga cucccgcugc cccacccagg gcgaggccua ccucgacaag      540

caguccgaca cccaguacgu cugcaagcgc acccucgucg accgcggcug gggcaacggc      600

ugcggccucu ucggcaaggg cucccucguc accugcgcca aguucgccug cuccaagaag      660

augaccggca aguccaucca gcccgagaac cucgaguacc gcaucaugcu cuccguccac      720

ggcucccagc acuccggcau gaucgucaac gacaccggcc acgagaccga cgagaaccgc      780

gccaaggucg agaucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc      840

ucccucggcc ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc      900

accaugaaca acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc      960

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag     1020

uucaaggacg cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc     1080

guccacaccg cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc     1140

uccuccggcc accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc     1200

uacucccucu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccuccacggc     1260

accgucaccg ucgaggucca guacgccggc accgacggcc ccugcaaggu ccccgcccag     1320

auggccgucg acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc     1380

aucaccgagu ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac     1440

uccuacaucg ucaucggcgu cggcgagaag aagaucaccc accacuggca ccgcuccggc     1500

uccaccaucg gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc     1560

ggcgacaccg ccugggacuu cggcuccguc ggcggcgccc ucaacucccu cggcaagggc     1620

auccaccaga ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc     1680

cagauccuca ucggcacccu ccucaugugg cucggccuca acaccaagaa cggcuccauc     1740

ucccucaugu gccucgcccu cggcggcguc cucaucuucc ucuccaccgc cgucuccgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10109
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10109
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gccgucaccc uccccuccca cuccacccgc      120

aagcuccaga cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc      180

cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc      240

uggcuccucg gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc      300

gcccccgccu acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug      360

uccggcggca ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc      420

caggacaagc ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag      480

guccgcuccu acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc      540

acccagggcg aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc      600

cucgucgacc gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc      660

ugcgccaagu ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc      720

gaguaccgca ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac      780

accggccacg agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc      840

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc      900

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag      960

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1020

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1080

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1140

gagauggacg gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug     1200

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1260

aagauccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1320

gacggccccu gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc     1380

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1440

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag     1500

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1560

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1620

ggcgcccuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1680

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc     1740

ggccucaaca ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc     1800

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10110
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10110
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gccgucaccc uccccuccca cuccacccgc      120

aagcuccaga cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc      180

cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc      240

uggcuccucg gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc      300

gcccccgccu acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug      360

uccggcggca ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc      420

caggacaagc ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag      480

guccgcuccu acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc      540

acccagggcg aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc      600

cucgucgacc gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc      660

ugcgccaagu ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc      720

gaguaccgca ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac      780

accggccacg agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc      840

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc      900

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag      960

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1020

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1080

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1140

gagauggacg gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug     1200

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1260

aagauccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1320

gacggccccu gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc     1380

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1440

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag     1500

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1560

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1620

ggcgcccuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1680

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc     1740

ggccucaacg ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc     1800

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10111
<211>  2020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10111
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gccgucaccc uccccuccca cuccacccgc      120

aagcuccaga cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc      180

aaggucgaga acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc      240

uggcuccucg gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc      300

gcccccgccu acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug      360

uccggcggca ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc      420

caggacaagc ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag      480

guccgcuccu acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc      540

acccagggcg aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc      600

cucgucgacc gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc      660

ugcgccaagu ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc      720

gaguaccgca ucaugcucuc cguccacggc ucccagcacu ccggcaugau cggcuacgag      780

accgacgagg accgcgccaa ggucgagguc acccccaacu ccccccgcgc cgaggccacc      840

cucggcggcu ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc      900

gaccucuacu accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac      960

gacauccccc uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1020

gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc     1080

ucccaggagg gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc     1140

gccaagggcc gccucuucuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc     1200

cucaagggcg ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gguccccgcc     1260

gagacccucc acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc     1320

aagauccccg uccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc     1380

accgccaacc ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac     1440

ccccccuucg gcgacuccua caucgucauc ggcgucggcg acaagaagau cacccaccac     1500

uggcaccgcu ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag     1560

cgcauggccg uccucggcga caccgccugg gacuucggcu ccgucggcgg cgucuucaac     1620

ucccucggca agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc     1680

auguccuggu ucucccagau ccucaucggc acccuccucg ucuggcucgg ccucaacacc     1740

aagaacggcu ccaucucccu caccugccuc gcccucggcg gcgucaugau cuuccucucc     1800

accgccgucu ccgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     1860

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1980

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2020


<210>  10112
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10112
gggagaaagc uuaccauggc caucuccggc guccccgucc ucggcuucuu caucaucgcc       60

guccucaugu ccgcccagga guccugggcc gccgucaccc uccccuccca cuccacccgc      120

aagcuccaga cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc      180

cgcgucgaga acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc      240

uggcuccucg gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc      300

gcccccgccu acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug      360

uccggcggca ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc      420

caggacaagc ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag      480

guccgcuccu acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc      540

acccagggcg aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc      600

cucgucgacc gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc      660

ugcgccaagu ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc      720

gaguaccgca ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac      780

accggccacg agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc      840

gccgaggcca cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc      900

cucgacuucu ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag      960

gagugguucc acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac     1020

uggaacaaca aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc     1080

gucguccucg gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc     1140

gagauggacg gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug     1200

gacaagcucc gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc     1260

aagauccccg ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc     1320

gacggccccu gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc     1380

ggccgccuca ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug     1440

cucgagcucg acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag     1500

aucacccacc acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc     1560

cgcggcgcca agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc     1620

ggcgcccuca acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc     1680

cucuucggcg gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc     1740

ggccucaaca ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc     1800

aucuuccucu ccaccgccgu cuccgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10113
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10113
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      120

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      180

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      240

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      300

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      360

aucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      420

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      480

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      540

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      600

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      660

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      720

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      780

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      840

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      900

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      960

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1020

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1080

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1140

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1200

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1260

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1320

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1380

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1440

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1500

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1560

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1620

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1680

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     1740

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     1800

uccgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10114
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10114
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      120

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      180

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      240

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      300

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      360

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      420

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      480

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      540

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      600

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      660

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      720

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      780

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      840

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      900

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      960

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1020

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1080

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1140

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1200

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1260

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1320

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1380

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1440

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1500

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1560

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1620

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1680

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacgc caagaacggc     1740

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     1800

uccgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10115
<211>  1999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10115
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      120

accuggcucg agucccgcga guacaccaag caccucauca aggucgagaa cuggaucuuc      180

cgcaaccccg gcuucgcccu cgucgccguc gccaucgccu ggcuccucgg cuccuccacc      240

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      300

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      360

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      420

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      480

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      540

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      600

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu caccugcucc      660

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      720

guccacggcu cccagcacuc cggcaugauc ggcuacgaga ccgacgagga ccgcgccaag      780

gucgagguca cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc      840

ggccucgacu gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug      900

aacaacaagc acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac      960

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag     1020

gacgcccacg ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac     1080

accgcccucg ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuucucc     1140

ggccaccuca agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc     1200

cucugcaccg ccgccuucac cuucaccaag guccccgccg agacccucca cggcaccguc     1260

accgucgagg uccaguacgc cggcaccgac ggccccugca agauccccgu ccagauggcc     1320

gucgacaugc agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc     1380

gaguccaccg agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac     1440

aucgucaucg gcgucggcga caagaagauc acccaccacu ggcaccgcuc cggcuccacc     1500

aucggcaagg ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac     1560

accgccuggg acuucggcuc cgucggcggc gucuucaacu cccucggcaa gggcauccac     1620

cagaucuucg gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc     1680

cucaucggca cccuccucgu cuggcucggc cucaacacca agaacggcuc caucucccuc     1740

accugccucg cccucggcgg cgucaugauc uuccucucca ccgccgucuc cgccugagga     1800

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     1860

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     1980

uuucagagcc accagaauu                                                  1999


<210>  10116
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10116
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucgucgccgc cgccacccgc       60

guccacuccg ccgucacccu ccccucccac uccacccgca agcuccagac ccgcucccag      120

accuggcucg agucccgcga guacaccaag caccucaucc gcgucgagaa cuggaucuuc      180

cgcaaccccg gcuucgcccu cgccgccgcc gccaucgccu ggcuccucgg cuccuccacc      240

ucccagaagg ucaucuaccu cgucaugauc cuccucaucg cccccgccua cuccauccgc      300

ugcaucggcg ucuccaaccg cgacuucguc gagggcaugu ccggcggcac cugggucgac      360

gucguccucg agcacggcgg cugcgucacc gucauggccc aggacaagcc caccgucgac      420

aucgagcucg ucaccaccac cgucuccaac auggccgagg uccgcuccua cugcuacgag      480

gccuccaucu ccgacauggc cuccgacucc cgcugcccca cccagggcga ggccuaccuc      540

gacaagcagu ccgacaccca guacgucugc aagcgcaccc ucgucgaccg cggcuggggc      600

aacggcugcg gccucuucgg caagggcucc cucgucaccu gcgccaaguu cgccugcucc      660

aagaagauga ccggcaaguc cauccagccc gagaaccucg aguaccgcau caugcucucc      720

guccacggcu cccagcacuc cggcaugauc gucaacgaca ccggccacga gaccgacgag      780

aaccgcgcca aggucgagau cacccccaac uccccccgcg ccgaggccac ccucggcggc      840

uucggcuccc ucggccucga cugcgagccc cgcaccggcc ucgacuucuc cgaccucuac      900

uaccucacca ugaacaacaa gcacuggcuc guccacaagg agugguucca cgacaucccc      960

cuccccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccuc     1020

gucgaguuca aggacgccca cgccaagcgc cagaccgucg ucguccucgg cucccaggag     1080

ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg agauggacgg cgccaagggc     1140

cgccucuccu ccggccaccu caagugccgc cucaagaugg acaagcuccg ccucaagggc     1200

gucuccuacu cccucugcac cgccgccuuc accuucacca agauccccgc cgagacccuc     1260

cacggcaccg ucaccgucga gguccaguac gccggcaccg acggccccug caaggucccc     1320

gcccagaugg ccgucgacau gcagacccuc acccccgucg gccgccucau caccgccaac     1380

cccgucauca ccgaguccac cgagaacucc aagaugaugc ucgagcucga cccccccuuc     1440

ggcgacuccu acaucgucau cggcgucggc gagaagaaga ucacccacca cuggcaccgc     1500

uccggcucca ccaucggcaa ggccuucgag gccaccgucc gcggcgccaa gcgcauggcc     1560

guccucggcg acaccgccug ggacuucggc uccgucggcg gcgcccucaa cucccucggc     1620

aagggcaucc accagaucuu cggcgccgcc uucaaguccc ucuucggcgg cauguccugg     1680

uucucccaga uccucaucgg cacccuccuc auguggcucg gccucaacac caagaacggc     1740

uccaucuccc ucaugugccu cgcccucggc ggcguccuca ucuuccucuc caccgccguc     1800

uccgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10117
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10117
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccgcc gucacccucc ccucccacuc cacccgcaag      120

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      180

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      240

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      300

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      360

ggcggcaccu gggucgacau cguccucgag cacggcggcu gcgucaccgu cauggcccag      420

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      480

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      540

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      600

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      660

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      720

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      780

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      840

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      900

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      960

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1020

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1080

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1140

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1200

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1260

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1320

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1380

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1440

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1500

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1560

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1620

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1680

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1740

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     1800

uuccucucca ccgccgucuc cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10118
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10118
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccgcc gucacccucc ccucccacuc cacccgcaag      120

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      180

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      240

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      300

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      360

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      420

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      480

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      540

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      600

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      660

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      720

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      780

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      840

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      900

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      960

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1020

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1080

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1140

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1200

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1260

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1320

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1380

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1440

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1500

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1560

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1620

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1680

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1740

cucaacgcca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     1800

uuccucucca ccgccgucuc cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10119
<211>  2017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10119
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccgcc gucacccucc ccucccacuc cacccgcaag      120

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucaucaag      180

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ucgccgucgc caucgccugg      240

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      300

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      360

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      420

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      480

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      540

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      600

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      660

gccaaguuca ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      720

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgg cuacgagacc      780

gacgaggacc gcgccaaggu cgaggucacc cccaacuccc cccgcgccga ggccacccuc      840

ggcggcuucg gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac      900

cucuacuacc ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac      960

aucccccucc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1020

gcccucgucg aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc     1080

caggagggcg ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc     1140

aagggccgcc ucuucuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc     1200

aagggcgucu ccuacucccu cugcaccgcc gccuucaccu ucaccaaggu ccccgccgag     1260

acccuccacg gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag     1320

auccccgucc agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc     1380

gccaaccccg ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc     1440

cccuucggcg acuccuacau cgucaucggc gucggcgaca agaagaucac ccaccacugg     1500

caccgcuccg gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc     1560

auggccgucc ucggcgacac cgccugggac uucggcuccg ucggcggcgu cuucaacucc     1620

cucggcaagg gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug     1680

uccugguucu cccagauccu caucggcacc cuccucgucu ggcucggccu caacaccaag     1740

aacggcucca ucucccucac cugccucgcc cucggcggcg ucaugaucuu ccucuccacc     1800

gccgucuccg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     1860

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1980

ccccccccca aaggcucuuu ucagagccac cagaauu                              2017


<210>  10120
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10120
gggagaaagc uuaccauggg caagcgcucc gccggcucca ucauguggcu cgccucccuc       60

gccgucguca ucgccugcgc cggcgccgcc gucacccucc ccucccacuc cacccgcaag      120

cuccagaccc gcucccagac cuggcucgag ucccgcgagu acaccaagca ccucauccgc      180

gucgagaacu ggaucuuccg caaccccggc uucgcccucg ccgccgccgc caucgccugg      240

cuccucggcu ccuccaccuc ccagaagguc aucuaccucg ucaugauccu ccucaucgcc      300

cccgccuacu ccauccgcug caucggcguc uccaaccgcg acuucgucga gggcaugucc      360

ggcggcaccu gggucgacgu cguccucgag cacggcggcu gcgucaccgu cauggcccag      420

gacaagccca ccgucgacau cgagcucguc accaccaccg ucuccaacau ggccgagguc      480

cgcuccuacu gcuacgaggc cuccaucucc gacauggccu ccgacucccg cugccccacc      540

cagggcgagg ccuaccucga caagcagucc gacacccagu acgucugcaa gcgcacccuc      600

gucgaccgcg gcuggggcaa cggcugcggc cucuucggca agggcucccu cgucaccugc      660

gccaaguucg ccugcuccaa gaagaugacc ggcaagucca uccagcccga gaaccucgag      720

uaccgcauca ugcucuccgu ccacggcucc cagcacuccg gcaugaucgu caacgacacc      780

ggccacgaga ccgacgagaa ccgcgccaag gucgagauca cccccaacuc cccccgcgcc      840

gaggccaccc ucggcggcuu cggcucccuc ggccucgacu gcgagccccg caccggccuc      900

gacuucuccg accucuacua ccucaccaug aacaacaagc acuggcucgu ccacaaggag      960

ugguuccacg acaucccccu ccccuggcac gccggcgccg acaccggcac cccccacugg     1020

aacaacaagg aggcccucgu cgaguucaag gacgcccacg ccaagcgcca gaccgucguc     1080

guccucggcu cccaggaggg cgccguccac accgcccucg ccggcgcccu cgaggccgag     1140

auggacggcg ccaagggccg ccucuccucc ggccaccuca agugccgccu caagauggac     1200

aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg ccgccuucac cuucaccaag     1260

auccccgccg agacccucca cggcaccguc accgucgagg uccaguacgc cggcaccgac     1320

ggccccugca agguccccgc ccagauggcc gucgacaugc agacccucac ccccgucggc     1380

cgccucauca ccgccaaccc cgucaucacc gaguccaccg agaacuccaa gaugaugcuc     1440

gagcucgacc cccccuucgg cgacuccuac aucgucaucg gcgucggcga gaagaagauc     1500

acccaccacu ggcaccgcuc cggcuccacc aucggcaagg ccuucgaggc caccguccgc     1560

ggcgccaagc gcauggccgu ccucggcgac accgccuggg acuucggcuc cgucggcggc     1620

gcccucaacu cccucggcaa gggcauccac cagaucuucg gcgccgccuu caagucccuc     1680

uucggcggca uguccugguu cucccagauc cucaucggca cccuccucau guggcucggc     1740

cucaacacca agaacggcuc caucucccuc augugccucg cccucggcgg cguccucauc     1800

uuccucucca ccgccgucuc cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10121
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10121
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gugaccagaa ggggaucagc guacuauaug      120

uaccuugacc ggaacgaugc uggugaagcc aucaguuucc cuacaacucu gggcaugaau      180

aagugcuaua uucaaaucau ggacuugggg cacaugugug augcaacgau gagcuacgag      240

ugcccaaugu uagacgaagg aguagagccg gaugacgugg auuguuggug caacaccaca      300

uccacuuggg ucguguaugg caccugucau cacaaaaagg gugaagcccg ccgaucucgu      360

agagcuguua cacugcccuc gcauucaacc aggaaacuac agacucggag ccagacaugg      420

cucgagaguc gcgaguacac caagcaccug auuagagugg aaaauuggau cuuuaggaac      480

ccuggguucg cacuugccgc ugccgcgaua gcauggcugu ugggauccuc uacgagccag      540

aaagucaucu aucucgugau gauucugcug aucgccccag cuuacuccau ucggugcauc      600

ggcguaucaa aucgcgacuu uguugagggc augagugggg gaacuugggu ggauauaguc      660

cucgaacaug guggcugugu gaccgugaug gcccaagaca agcccacagu cgauauugag      720

uuaguuacua ccacagugag caacauggca gagguacgau cuuacugcua ugaggcuucc      780

aucucagaca uggccagcga uaguagaugu ccuacccagg gggaagcgua ccuggacaag      840

cagucugaca cgcaguaugu gugcaaaagg acucuugucg aucggggaug gggcaauggu      900

uguggguugu ucggaaaggg cucccuggug acaugcgcca aauuugcaug cagcaagaaa      960

augaccggca agucgauuca accagaaaac cuagaguacc guaucaugcu cucuguucac     1020

gggucccagc acucaggaau gaucgugaau gacacugguc augaaacaga ugagaaccgc     1080

gcuaaggucg agauuacccc caauaguccu agagccgaag cuacgcuggg cggguucgga     1140

agccuuggcc uggacuguga gccaaggacc gguuuggauu uuuccgaccu cuauuaccug     1200

acaaugaaca auaaacacug guuagugcau aaggaauggu uccacgauau accgcugccc     1260

uggcacgccg gggcagacac uggcaccccu cauuggaaca auaaagaggc ccucguagaa     1320

uuuaaggaug cucacgcgaa acggcagaca guuguggucc uuggaucuca ggagggcgca     1380

gugcauacug cccuggcugg ggccuuggag gcagaaaugg acggagccaa gggucgacua     1440

agcucaggcc accugaaaug ccgccucaag auggauaagc ugagacuuaa aggggucagu     1500

uauucccugu guaccgcugc cuucacauuu accaagaucc cagcagagac uuugcaugga     1560

acggugacag uugaagugca auacgcgggc accgacgguc ccugcaaagu accugcucag     1620

auggccgucg acaugcagac ucucacccca guggggaggc ugauuacagc aaaccccguu     1680

aucaccgaga gcacugaaaa cucuaagaug auguuagagc uggauccgcc uuucggcgac     1740

ucauacauag ugauuggagu cggcgagaaa aagaucacac accacuggca ucggaguggg     1800

agcacgaucg gaaaggccuu cgaagcuacc gugcgcggug ccaaaagaau ggcaguacua     1860

ggcgauacug cuugggacuu uggguccgug ggaggcgccc ucaauucucu ggguaagggg     1920

auucaccaaa ucuucggcgc ggccuuuaaa ucgcuuuucg gaggcaugag cugguuuucc     1980

cagauuuuga uagggacacu gcucaugugg cugggacuua acaccaagaa uggcucaauc     2040

agucugaugu guuuggcucu cggugggguu cugauuuucu uaucuacagc agucagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10122
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10122
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gugaccagaa ggggaucagc guacuauaug      120

uaccuugacc ggaacgaugc uggugaagcc aucaguuucc cuacaacucu gggcaugaau      180

aagugcuaua uucaaaucau ggacuugggg cacacgugug augcaaccau gagcuacgag      240

ugcccaaugu uagacgaagg aguagagccg gaugacgugg auuguuggug caacacaacu      300

uccaccuggg ucguguaugg cacaugucau cacaaaaagg gugaagcccg ccgaucucgu      360

agagcuguua cccugcccuc gcauucaacu aggaaacuac agacacggag ccagaccugg      420

cucgagaguc gcgaguacac gaagcaccug auuagagugg aaaauuggau cuuuaggaac      480

ccuggguucg cacuugccgc ugccgcgaua gcauggcugu ugggauccuc uacuagccag      540

aaagucaucu aucucgugau gauucugcug aucgccccag cuuacuccau ucggugcauc      600

ggcguaucaa aucgcgacuu uguugagggc augagugggg gaaccugggu ggaugucgug      660

cucgaacaug guggcugugu gacagucaug gcccaagaca agcccacugu ugauauagag      720

uuagugacca caaccguaag caacauggca gaggugcgau cuuacugcua ugaggcuucc      780

auuucagaca uggccagcga uaguagaugu ccuacgcagg gggaagcgua ccuggacaag      840

cagucugaca cucaguaugu cugcaaaagg acacuugugg aucggggaug gggcaauggu      900

uguggguugu ucggaaaggg cucccugguu accugcgcca aauuugcaug cagcaagaaa      960

augacuggca agucgaucca accagaaaac cuagaguacc guauuaugcu cucugugcac     1020

gggucccagc acucaggaau gaucgucaau gacacagguc augaaaccga ugagaaccgc     1080

gcuaaggugg agaucacgcc caauaguccu agagccgaag cuacccuggg cggguucgga     1140

agccuuggcc uggacuguga gccaaggaca gguuuggauu uuuccgaccu cuauuaccug     1200

acuaugaaca auaaacacug guuaguacau aaggaauggu uccacgauau uccgcugccc     1260

uggcacgccg gggcagacac cggcacaccu cauuggaaca auaaagaggc ccucguugaa     1320

uuuaaggaug cucacgcgaa acggcagacu guggucgugc uuggaucuca ggagggcgca     1380

guccauaccg cccuggcugg ggccuuggag gcagaaaugg acggagccaa gggucgacua     1440

agcucaggcc accugaaaug ccgccucaag auggauaagc ugagacuuaa aggggugagu     1500

uauucccugu guacagcugc cuucaccuuu acuaagauac cagcagagac guugcaugga     1560

acaguuaccg uggaaguaca auacgcgggc acugacgguc ccugcaaagu cccugcucag     1620

auggccgugg acaugcagac ccucacacca guugggaggc ugaucaccgc aaaccccgug     1680

auuacugaga gcacagaaaa cucuaagaug auguuagagc uggauccgcc uuucggcgac     1740

ucauacaucg ucauaggagu gggcgagaaa aagauuacgc accacuggca ucggaguggg     1800

agcaccaucg gaaaggccuu cgaagcuacu guacgcggug ccaaaagaau ggcagugcua     1860

ggcgauacag cuugggacuu uggguccguu ggaggcgccc ucaauucucu ggguaagggg     1920

auccaccaaa uuuucggcgc ggccuuuaaa ucgcuuuucg gaggcaugag cugguuuucc     1980

cagaucuuga uugggacccu gcucaugugg cugggacuua acgcuaagaa uggcucaaua     2040

agucugaugu guuuggcacu cggugggguc cugaucuucu uaucuacagc cgugagcgcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10123
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10123
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc aucaccagaa ggggaucagc guacuauaug      120

uaccuugacc ggagugaugc ugguaaggcc auuagcuucg caacaacucu gggcgugaac      180

aaaugccacg uacaaaucau ggacuugggg cauaugugug augccacgau guccuaugaa      240

ugcccuaugu uagacgaggg aguggaacca gaugacgucg auuguuggug caauaccaca      300

ucuacuuggg ugguuuacgg caccugucac cauaagaaag gugaggcucg ccgaucgcgu      360

agagcaguga cacugccguc acacagcacc aggaagcuac agacucggag ucagacaugg      420

cucgaauccc gcgaguauac caaacaucug auuaaggucg agaacuggau cuuuagaaau      480

cccggguucg cccuuguggc uguagccaua gcguggcugu ugggaucuag cacgucccag      540

aagguuaucu accucgugau gauucugcug aucgcacccg ccuauucaau uaggugcauc      600

ggcgucagua accgggacuu uguggaaggc augagcgggg gaacuugggu ggaugucguu      660

cucgagcacg guggcugugu gaccguaaug gcucaagaca aaccaacagu ggauauagaa      720

uuagucacua ccacaguguc uaauauggcc gagguucgcu ccuacugcua cgaggcauca      780

auuagcgaca uggcuaguga uucucgaugu cccacccagg gggaagccua ucuggacaag      840

caguccgaca cgcaguacgu gugcaaaaga acucuugucg auaggggaug gggcaacggu      900

uguggguugu ucggaaaggg cagccuggug acaugcgcga aauuuaccug cucgaagaag      960

augacuggca aaucuaucca accugagaau cuagaauauc ggauuaugcu cuccguacac     1020

gggucacagc auaguggaau gaucgguuac gagacagacg aagaucgugc caagguugag     1080

gugaccccaa acagcccccg cgcagaggcu acgcugggcg gguucggauc ccuuggccug     1140

gacugugaac cuagaaccgg uuuggauuuu ucugaccucu auuaccugac aaugaauaac     1200

aaacacuggu uaguccauaa ggagugguuc cacgauaucc cacugccgug gcacgccggg     1260

gcugacacug gcaccccuca uuggaauaac aaagaagccc ucguggaguu uaaggaugca     1320

cacgccaaaa ggcagacagu cgugguucuu ggaagccagg aaggcgcugu gcauacugcg     1380

cuggcagggg ccuuggaggc ugagauggac ggagccaagg gucggcuauu cucaggccac     1440

cugaagugcc gacucaaaau ggauaagcug cgccuuaaag ggguaaguua uucccugugu     1500

accgcagccu uuacauucac caaggucccu gcugaaacuu ugcauggaac ggugacaguu     1560

gaggugcaau acgccggcac cgacggucca ugcaaaauuc ccguccagau ggcaguggac     1620

augcagacuc ucaccccugu agggagacug auaacagcga auccagugau caccgaaagc     1680

acugagaacu cuaagaugau guuagaacug gaucccccgu ucggcgacuc auacauuguu     1740

aucggagucg gcgauaagaa aauaacacac cacuggcaua ggagugggag cacgauugga     1800

aaggcuuuug aggccaccgu gcggggugca aaacgcaugg ccgugcuagg cgacacugcu     1860

ugggauuucg gguccgucgg aggcguuuuu aauucucucg guaaggggau ccaccaaauc     1920

uucggcgccg cauuuaaauc gcuguucgga ggcaugagcu gguuuuccca gauucuuauc     1980

gggacauugc ugguguggcu cggacugaac accaagaacg gcucaauuag ucuuacaugu     2040

cuggcuuugg guggggugau gauauuccuc ucuacugccg uaagcgcgug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10124
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10124
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gugaccagaa ggggaucagc guacuauaug      120

uaccuugacc ggaacgaugc uggugaagcc aucaguuucc cuacaacucu gggcaugaau      180

aagugcuaua uucaaaucau ggacuugggg cacaugugug augcaacgau gagcuacgag      240

ugcccaaugu uagacgaagg aguagagccg gaugacgugg auuguuggug caacaccaca      300

uccacuuggg ucguguaugg caccugucau cacaaaaagg gugaagcccg ccgaucucgu      360

agagcuguua cacugcccuc gcauucaacc aggaaacuac agacucggag ccagacaugg      420

cucgagaguc gcgaguacac caagcaccug auuagagugg aaaauuggau cuuuaggaac      480

ccuggguucg cacuugccgc ugccgcgaua gcauggcugu ugggauccuc uacgagccag      540

aaagucaucu aucucgugau gauucugcug aucgccccag cuuacuccau ucggugcauc      600

ggcguaucaa aucgcgacuu uguugagggc augagugggg gaacuugggu ggaugucgug      660

cucgaacaug guggcugugu gaccgucaug gcccaagaca agcccacagu ugauauagag      720

uuagugacua ccacaguaag caacauggca gaggugcgau cuuacugcua ugaggcuucc      780

auuucagaca uggccagcga uaguagaugu ccuacccagg gggaagcgua ccuggacaag      840

cagucugaca cgcaguaugu cugcaaaagg acucuugugg aucggggaug gggcaauggu      900

uguggguugu ucggaaaggg cucccugguu acaugcgcca aauuugcaug cagcaagaaa      960

augaccggca agucgaucca accagaaaac cuagaguacc guauuaugcu cucugugcac     1020

gggucccagc acucaggaau gaucgucaau gacacugguc augaaacaga ugagaaccgc     1080

gcuaaggugg agaucacccc caauaguccu agagccgaag cuacgcuggg cggguucgga     1140

agccuuggcc uggacuguga gccaaggacc gguuuggauu uuuccgaccu cuauuaccug     1200

acaaugaaca auaaacacug guuaguacau aaggaauggu uccacgauau uccgcugccc     1260

uggcacgccg gggcagacac uggcaccccu cauuggaaca auaaagaggc ccucguugaa     1320

uuuaaggaug cucacgcgaa acggcagaca guggucgugc uuggaucuca ggagggcgca     1380

guccauacug cccuggcugg ggccuuggag gcagaaaugg acggagccaa gggucgacua     1440

agcucaggcc accugaaaug ccgccucaag auggauaagc ugagacuuaa aggggugagu     1500

uauucccugu guaccgcugc cuucacauuu accaagauac cagcagagac uuugcaugga     1560

acgguuacag uggaaguaca auacgcgggc accgacgguc ccugcaaagu cccugcucag     1620

auggccgugg acaugcagac ucucacccca guugggaggc ugaucacagc aaaccccgug     1680

auuaccgaga gcacugaaaa cucuaagaug auguuagagc uggauccgcc uuucggcgac     1740

ucauacaucg ucauaggagu gggcgagaaa aagauuacac accacuggca ucggaguggg     1800

agcacgaucg gaaaggccuu cgaagcuacc guacgcggug ccaaaagaau ggcagugcua     1860

ggcgauacug cuugggacuu uggguccguu ggaggcgccc ucaauucucu ggguaagggg     1920

auccaccaaa uuuucggcgc ggccuuuaaa ucgcuuuucg gaggcaugag cugguuuucc     1980

cagaucuuga uugggacacu gcucaugugg cugggacuua acaccaagaa uggcucaaua     2040

agucugaugu guuuggcucu cggugggguc cugaucuucu uaucuacagc agugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10125
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10125
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagcg uuacuaggcg gggcuccgcc uacuauaugu accuugaucg caacgacgcg      120

ggggaggcua uuucuuuucc cacgacccug ggaaugaaua agugcuauau acagaucaug      180

gauuuggguc auauguguga cgccacaaug ucauacgaau gcccuauguu agaugagggc      240

guggaaccag acgauguaga cuguuggugc aacacuacca guacaugggu ggucuauggg      300

accugucacc auaaaaaggg agaggcacga cguagcagaa gggccgugac ucugccgucc      360

cacucuacac ggaaacuaca aacccgcucg cagacguggc ucgaaucaag agaguacacu      420

aagcaucuga uuaggguuga gaauuggauc uuccggaacc ccggcuuugc ucuugcagcc      480

gcugccauug cguggcuguu ggguagcagu accucccaga aagugaucua ucucgucaug      540

auacugcuga ucgcacccgc cuacucuauu cgcugcaucg gggugagcaa ucgagauuuc      600

guagaaggaa uguccggcgg cacauggguu gacauugugc ucgagcacgg gggauguguc      660

acugugaugg cucaggauaa gccaaccgug gacaucgaau uagucacaac cacgguuuca      720

aacauggccg aggugagaag uuacugcuau gaggcaagca uaucugauau ggcuuccgac      780

ucaagguguc ccacucaagg ugaagccuac cuggacaagc agagcgauac acaguaugua      840

ugcaaacgga cccuugugga ccguggcugg gggaauggau guggcuuguu ugguaagggg      900

agucugguca cuugcgcgaa auucgccugc ucuaagaaaa ugacaggaaa guccauucag      960

ccugagaacc uagaauaccg caucaugcuc agcgugcacg gcucgcaaca uucuggcaug     1020

auuguuaaug auaccgggca cgagacggac gaaaacagag caaaggugga gaucacccca     1080

aauuccccca gggcugaggc cacacuggga gguuuuggcu cacuugggcu ggauugugaa     1140

ccucggacug gauuggacuu cagugaucuc uauuaccuga ccaugaacaa uaaacauugg     1200

uuaguccaca aggagugguu ucacgacauc ccacugccgu ggcaugcugg cgccgauaca     1260

gguacucccc acuggaacaa uaaagaagca cucguggagu ucaaggacgc ccaugcuaaa     1320

cgacagaccg uaguugugcu ugggagccag gaaggcgcgg uccacacagc acuggccgga     1380

gcuuuggagg ccgagaugga uggcgcaaag gggcgccuau ccucuggaca ucugaaaugc     1440

agacucaaga uggacaagcu gaggcuuaaa ggugugagcu auucacugug uaccgccgcu     1500

uuuacuuuca cgaagauucc ugccgaaaca uugcacggca ccgucacugu ggagguucag     1560

uacgcaggga ccgacggacc augcaaagug cccgcgcaaa uggcuguaga uaugcagaca     1620

cucaccccug ucggccggcu gauaacugcc aacccaguga ucacagaaag uacggagaac     1680

uccaagauga uguuagaacu ggacccuccg uuuggugaua gcuacauugu uaucggggug     1740

ggcgagaaaa agauaaccca ccauuggcac cgcucuggau caacuauugg caaggcauuc     1800

gaggccacag ucagaggggc uaaaaggaug gccgugcuag gagacaccgc augggauuuc     1860

gguaguguag gcggggcucu caauagccug ggaaagggca uccaucagau cuuuggugcc     1920

gcguucaaau cccuuuuugg gggcaugucu ugguucucgc aaauuuugau cggaacacug     1980

cucauguggc ugggccuuaa cacuaagaau gggagcauuu cccugaugug uuuggcccuc     2040

ggaggcgugc ugauauuuuu aucaaccgcu guuagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10126
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10126
gggagaaagc uuaccaugga cuggaccugg auucuguucc ucguggccgc ugcaacaaga       60

guccacagcg uuacuaggcg gggcuccgcc uacuauaugu accuugaucg caacgacgcg      120

ggggaggcua uuucuuuucc cacgacccug ggaaugaaua agugcuauau acagaucaug      180

gauuuggguc auacauguga cgccacuaug ucauacgaau gcccuauguu agaugagggc      240

guggaaccag acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggg      300

acuugucacc auaaaaaggg agaggcacga cguagcagaa gggccgugac acugccgucc      360

cacucuaccc ggaaacuaca aacgcgcucg cagacuuggc ucgaaucaag agaguacacc      420

aagcaucuga uuaggguuga gaauuggauc uuccggaacc ccggcuuugc ucuugcagcc      480

gcugccauug cguggcuguu ggguagcagu acaucccaga aagugaucua ucucgucaug      540

auacugcuga ucgcacccgc cuacucuauu cgcugcaucg gggugagcaa ucgagauuuc      600

guagaaggaa uguccggcgg cacuuggguu gacguggucc ucgagcacgg gggaugugug      660

accgugaugg cucaggauaa gccaacaguc gacauugaau uaguuaccac gacuguguca      720

aacauggccg agguaagaag uuacugcuau gaggcaagca ucucugauau ggcuuccgac      780

ucaagguguc ccacacaagg ugaagccuac cuggacaagc agagcgauac ccaguaugug      840

ugcaaacgga cucuugucga ccguggcugg gggaauggau guggcuuguu ugguaagggg      900

agucugguga caugcgcgaa auucgccugc ucuaagaaaa ugaccggaaa guccauacag      960

ccugagaacc uagaauaccg cauuaugcuc agcguucacg gcucgcaaca uucuggcaug     1020

aucgugaaug auacggggca cgagaccgac gaaaacagag caaaggucga gauuacacca     1080

aauuccccca gggcugaggc cacucuggga gguuuuggcu cacuugggcu ggauugugaa     1140

ccucggaccg gauuggacuu cagugaucuc uauuaccuga caaugaacaa uaaacauugg     1200

uuagugcaca aggagugguu ucacgacauc ccacugccgu ggcaugcugg cgccgauacu     1260

gguaccccuc acuggaacaa uaaagaagca cucguagagu ucaaggacgc ccaugcuaaa     1320

cgacagacag uugugguccu ugggagccag gaaggcgcgg ugcacaccgc acuggccgga     1380

gcuuuggagg ccgagaugga uggcgcaaag gggcgccuau ccucuggaca ucugaaaugc     1440

agacucaaga uggacaagcu gaggcuuaaa ggugucagcu auucacugug uacugccgcu     1500

uuuacguuca caaagauccc ugccgaaacc uugcacggca cugugaccgu ugaggugcag     1560

uacgcaggga cagacggacc augcaaagua cccgcgcaaa uggcugucga uaugcagacc     1620

cucacuccug ugggccggcu gauuacagcc aacccaguua uaacggaaag uaccgagaac     1680

uccaagauga uguuagaacu ggaucccccg uuuggugaca gcuacaucgu gauugggguc     1740

ggcgagaaaa agaucacuca ccauuggcac cgcucuggau caacaauagg caaggcauuc     1800

gaggccaccg ugagaggggc uaaaaggaug gccguacuag gagacacagc augggauuuc     1860

gguagugugg gcggggcucu caauagccug ggaaagggca uucaucagau cuuuggugcc     1920

gcguucaaau cccuuuuugg gggcaugucu ugguucucgc aaaucuugau uggaacucug     1980

cucauguggc ugggccuuaa cgccaagaau gggagcaucu cccugaugug uuuggcucuc     2040

ggaggcguuc ugaucuuuuu aucaaccgca gucagugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10127
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10127
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagca uuacuaggcg gggcuccgcc uacuauaugu accuugaucg cucugacgcg      120

gggaaggcua uaucauuugc cacgacccug ggaguuaaca aaugccaugu gcagaucaug      180

gauuuggguc acauguguga cgcaacaaug aguuaugagu gccccauguu agaugaaggc      240

guagagccug acgaugugga cuguuggugc aauacuacca gcacaugggu cguguacggg      300

accugucauc acaagaaagg agaagcccga cguuccagaa gggcuguuac ucugccaucu      360

cauucgacac ggaagcuaca aacccgcuca cagacguggc ucgagagcag agaauauacu      420

aaacaccuga uuaaggugga gaacuggauc uucaggaauc cgggcuuugc acuugucgcc      480

guggcuauug ccuggcuguu ggguaguucc accucucaga agguaaucua ccucguuaug      540

auacugcuga ucgcgcccgc auauagcauu cggugcaucg ggguguccaa ccgcgauuuc      600

gucgagggaa ugucaggcgg cacaugggug gacguggucc ucgaacacgg gggauguguu      660

acugugaugg cccaggauaa accuaccgua gacauugagu uagugacaac cacggucagu      720

aauauggcug aagugcgaag cuacugcuac gaggccucua ucuccgauau ggcaucagac      780

agcagauguc caacucaagg ugaggcuuau cuggacaagc agagugauac acaguacguu      840

ugcaaaagga cccuugugga ccggggcugg gggaacggau guggcuuguu ugguaagggg      900

ucucugguca cuugcgccaa auucacaugc uccaagaaga ugaccggaaa aagcauacag      960

cccgaaaauc uagaguaucg uauuaugcuc ucggugcaug gcucucaaca cuccggcaug     1020

aucggguacg aaacggauga ggaccgcgcg aagguagaag uuaccccuaa cucaccaaga     1080

gccgaggcaa cacugggagg uuuuggcagu cuugggcugg auugugagcc caggacugga     1140

uuggacuuca gcgaucucua uuaccugacc augaauaaca aacauugguu agugcacaag     1200

gaaugguuuc acgacauucc ucugccaugg caugcuggcg ccgauacagg uacuccgcac     1260

uggaauaaca aagaggcucu cgucgaguuc aaggacgccc augcaaaacg gcagaccgug     1320

gucgugcuug ggucccagga aggcgccguu cacacagcuc uggcgggagc auuggaagcc     1380

gagauggaug gcgcuaaggg gcgacuauuu ucuggacauc ugaagugccg ccucaaaaug     1440

gacaagcuga gacuuaaagg ugugagcuau ucacugugua ccgccgcauu cacuuuuacg     1500

aagguacccg ccgagacauu gcacggcacc gucacugugg aaguucagua cgcugggacc     1560

gacggaccuu gcaaaauccc agugcaaaug gccgucgaua ugcagacacu cacccccgug     1620

ggcaggcuga ucacugcaaa uccuguaauu acagagagua cggaaaacuc caagaugaug     1680

uuagagcugg acccacccuu cggugauagc uacauaguga ucgggguugg cgacaagaaa     1740

auuacccacc auuggcaccg gucuggauca acuaucggca aggcguucga agcuacaguc     1800

cgcggggcca aaagaauggc agugcuagga gauaccgccu gggacuuugg uagugugggc     1860

ggggucuuca auagccucgg aaagggcaua caucagauuu uuggugcugc cuucaaaucc     1920

cuguuugggg gcaugucuug guucucgcaa auccuuaucg gaacauugcu gguuuggcuc     1980

ggccugaaca cuaagaacgg gagcauuucc cuuaccuguc uggcauuggg aggcgugaug     2040

aucuuucucu caacggcugu gagugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10128
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10128
gggagaaagc uuaccaugga cuggaccugg auucuguucc ucguggccgc ugcaacaaga       60

guccacagcg uuacuaggcg gggcuccgcc uacuauaugu accuugaucg caacgacgcg      120

ggggaggcua uuucuuuucc cacgacccug ggaaugaaua agugcuauau acagaucaug      180

gauuuggguc auauguguga cgccacaaug ucauacgaau gcccuauguu agaugagggc      240

guggaaccag acgauguaga cuguuggugc aacacuacca guacaugggu ggucuauggg      300

accugucacc auaaaaaggg agaggcacga cguagcagaa gggccgugac ucugccgucc      360

cacucuacac ggaaacuaca aacccgcucg cagacguggc ucgaaucaag agaguacacu      420

aagcaucuga uuaggguuga gaauuggauc uuccggaacc ccggcuuugc ucuugcagcc      480

gcugccauug cguggcuguu ggguagcagu accucccaga aagugaucua ucucgucaug      540

auacugcuga ucgcacccgc cuacucuauu cgcugcaucg gggugagcaa ucgagauuuc      600

guagaaggaa uguccggcgg cacauggguu gacguggucc ucgagcacgg gggaugugug      660

acugugaugg cucaggauaa gccaaccguc gacauugaau uaguuacaac cacgguguca      720

aacauggccg agguaagaag uuacugcuau gaggcaagca ucucugauau ggcuuccgac      780

ucaagguguc ccacucaagg ugaagccuac cuggacaagc agagcgauac acaguaugug      840

ugcaaacgga cccuugucga ccguggcugg gggaauggau guggcuuguu ugguaagggg      900

agucugguga cuugcgcgaa auucgccugc ucuaagaaaa ugacaggaaa guccauacag      960

ccugagaacc uagaauaccg cauuaugcuc agcguucacg gcucgcaaca uucuggcaug     1020

aucgugaaug auaccgggca cgagacggac gaaaacagag caaaggucga gauuacccca     1080

aauuccccca gggcugaggc cacacuggga gguuuuggcu cacuugggcu ggauugugaa     1140

ccucggacug gauuggacuu cagugaucuc uauuaccuga ccaugaacaa uaaacauugg     1200

uuagugcaca aggagugguu ucacgacauc ccacugccgu ggcaugcugg cgccgauaca     1260

gguacucccc acuggaacaa uaaagaagca cucguagagu ucaaggacgc ccaugcuaaa     1320

cgacagaccg uugugguccu ugggagccag gaaggcgcgg ugcacacagc acuggccgga     1380

gcuuuggagg ccgagaugga uggcgcaaag gggcgccuau ccucuggaca ucugaaaugc     1440

agacucaaga uggacaagcu gaggcuuaaa ggugucagcu auucacugug uaccgccgcu     1500

uuuacuuuca cgaagauccc ugccgaaaca uugcacggca ccgugacugu ugaggugcag     1560

uacgcaggga ccgacggacc augcaaagua cccgcgcaaa uggcugucga uaugcagaca     1620

cucaccccug ugggccggcu gauuacugcc aacccaguua uaacagaaag uacggagaac     1680

uccaagauga uguuagaacu ggacccuccg uuuggugaua gcuacaucgu gauugggguc     1740

ggcgagaaaa agaucaccca ccauuggcac cgcucuggau caacuauagg caaggcauuc     1800

gaggccacag ugagaggggc uaaaaggaug gccguacuag gagacaccgc augggauuuc     1860

gguagugugg gcggggcucu caauagccug ggaaagggca uucaucagau cuuuggugcc     1920

gcguucaaau cccuuuuugg gggcaugucu ugguucucgc aaaucuugau uggaacacug     1980

cucauguggc ugggccuuaa cacuaagaau gggagcaucu cccugaugug uuuggcccuc     2040

ggaggcguuc ugaucuuuuu aucaaccgcu gucagugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10129
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10129
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcuguu accaggcggg guucagccua cuauauguac      120

cuugaccgca acgaugcagg cgaggccaua aguuucccca caacucuggg gaugaauaaa      180

uguuauaucc agauuaugga cuugggacac augugcgaug cuacgaugag cuacgaaugu      240

ccuauguuag acgagggcgu ggaaccagau gacguagauu gcugguguaa caccacaucc      300

acuugggugg ucuaugguac cugccaucac aagaaagggg aggcacgacg uucuagaagg      360

gccgugacac ugccgucgca uucaacccgg aagcuacaaa cucgcagcca gacauggcuc      420

gaaaguagag aguacaccaa acaccugauc aggguugaga auuggauuuu ucggaacccc      480

ggauucgcuc uugccgcggc agccaucgcu uggcuguugg gcuccucuac gagccagaag      540

gugauauauc ucgucaugau ccugcugauu gccccugcau acuccauccg cuguauuggc      600

gugucaaauc gagacuuugu agaagggaug aguggaggua cuuggguuga uaucgugcuc      660

gagcauggcg ggugcgucac cgugauggcu caggacaagc caacagugga uauagaauua      720

gucacuacca caguuagcaa cauggccgag gugagaucuu acuguuauga ggcguccauu      780

ucagacaugg ccagcgauag uaggugcccc acccaaggag aagcauaccu ggacaaacag      840

ucugacacgc aguauguaug uaagcggacu cuuguggauc guggcugggg uaaugggugc      900

ggauuguucg gcaaaggcuc ccuggucaca ugcgcuaagu uugccuguag caaaaagaug      960

accgggaagu cgauccagcc ugagaaccua gaauaccgca uuaugcucuc ugugcacgga     1020

ucccaacacu cagguaugau cguuaaugac acuggccaug agacagauga aaacagagcu     1080

aaaguggaga ucaccccaaa uagucccagg gccgaggcaa cgcuuggggg auucggcagc     1140

cugggucugg acugcgaacc ucggaccggg uuggauuuuu ccgaccucua uuaccugaca     1200

augaacaaua agcacugguu aguccauaaa gagugguucc acgauauucc acugccgugg     1260

cacgccggcg cugacacugg aacaccccau uggaacaaua aggaagcgcu cguggaguuu     1320

aaagaugcac acgccaagcg acagaccgua guugugcuug gcucucagga aggggcuguc     1380

cauacugccc uggcaggagc cuuggaggcu gagauggacg gugccaaagg ccgccuaagc     1440

ucagggcacc ugaaguguag acucaagaug gauaaacuga ggcuuaaggg agugaguuau     1500

ucccugugca ccgcagcguu cacauuuacc aaaauaccug cugaaacuuu gcauggcacg     1560

gucacagugg agguucagua cgccgguacc gacgggccau guaaggugcc cgcacaaaug     1620

gccguagaca ugcagacucu caccccuguc ggccggcuga ucacagcuaa cccagugauu     1680

accgaaagca cugagaacuc uaaaaugaug uuagaacugg aucccccguu cggagacuca     1740

uacaucguua uaggcguggg ggagaagaag auuacacacc acuggcaucg caguggaagc     1800

acgaucggua aagccuuuga ggcaaccguc agaggcgcua agaggauggc cgugcuaggg     1860

gauacugcgu gggacuucgg auccguaggc ggugcccuca auucucuggg gaaaggcauc     1920

caccagauuu ucggagcugc auuuaagucg cuuuucggcg ggaugagcug guuuucccaa     1980

aucuugauug gaacacugcu cauguggcug ggccuuaaca ccaaaaaugg uucaauaagu     2040

cugaugugcu uggcccucgg gggagugcug aucuucuuau cuacagcugu uagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10130
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10130
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcuguu accaggcggg guucagccua cuauauguac      120

cuugaccgca acgaugcagg cgaggccaua aguuucccca caacucuggg gaugaauaaa      180

uguuauaucc agauuaugga cuugggacac acgugcgaug cuaccaugag cuacgaaugu      240

ccuauguuag acgagggcgu ggaaccagau gacguagauu gcugguguaa cacaacuucc      300

accugggugg ucuaugguac augccaucac aagaaagggg aggcacgacg uucuagaagg      360

gccgugaccc ugccgucgca uucaacucgg aagcuacaaa cacgcagcca gaccuggcuc      420

gaaaguagag aguacacgaa acaccugauc aggguugaga auuggauuuu ucggaacccc      480

ggauucgcuc uugccgcggc agccaucgcu uggcuguugg gcuccucuac uagccagaag      540

gugauauauc ucgucaugau ccugcugauu gccccugcau acuccauccg cuguauuggc      600

gugucaaauc gagacuuugu agaagggaug aguggaggua ccuggguuga ugugguccuc      660

gagcauggcg ggugcgugac agugauggcu caggacaagc caacugucga uaucgaauua      720

guuaccacaa ccgugagcaa cauggccgag guaagaucuu acuguuauga ggcguccaua      780

ucagacaugg ccagcgauag uaggugcccc acgcaaggag aagcauaccu ggacaaacag      840

ucugacacuc aguaugugug uaagcggaca cuugucgauc guggcugggg uaaugggugc      900

ggauuguucg gcaaaggcuc ccuggugacc ugcgcuaagu uugccuguag caaaaagaug      960

acugggaagu cgauucagcc ugagaaccua gaauaccgca ucaugcucuc uguucacgga     1020

ucccaacacu cagguaugau ugugaaugac acaggccaug agaccgauga aaacagagcu     1080

aaagucgaga ucacgccaaa uagucccagg gccgaggcaa cccuuggggg auucggcagc     1140

cugggucugg acugcgaacc ucggacaggg uuggauuuuu ccgaccucua uuaccugacu     1200

augaacaaua agcacugguu agugcauaaa gagugguucc acgauauccc acugccgugg     1260

cacgccggcg cugacaccgg aacaccccau uggaacaaua aggaagcgcu cguagaguuu     1320

aaagaugcac acgccaagcg acagacuguu gugguccuug gcucucagga aggggcugug     1380

cauaccgccc uggcaggagc cuuggaggcu gagauggacg gugccaaagg ccgccuaagc     1440

ucagggcacc ugaaguguag acucaagaug gauaaacuga ggcuuaaggg agucaguuau     1500

ucccugugca cagcagcguu caccuuuacu aaaauuccug cugaaacguu gcauggcaca     1560

gugaccguug aggugcagua cgccgguacu gacgggccau guaagguacc cgcacaaaug     1620

gccgucgaca ugcagacccu cacaccugug ggccggcuga uaaccgcuaa cccaguuauc     1680

acugaaagca cagagaacuc uaaaaugaug uuagaacugg aucccccguu cggagacuca     1740

uacauuguga ucggcgucgg ggagaagaag auaacgcacc acuggcaucg caguggaagc     1800

accauuggua aagccuuuga ggcaacugug agaggcgcua agaggauggc cguacuaggg     1860

gauacagcgu gggacuucgg auccgugggc ggugcccuca auucucuggg gaaaggcauc     1920

caccagaucu ucggagcugc auuuaagucg cuuuucggcg ggaugagcug guuuucccaa     1980

auuuugaucg gaacccugcu cauguggcug ggccuuaacg ccaaaaaugg uucaauuagu     2040

cugaugugcu uggcucucgg gggaguucug auauucuuau cuacagccgu cagcgcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10131
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10131
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcuaua accaggcggg guucagccua cuauauguac      120

cuugaccgca gugaugcagg caaagccauc agcuucgcua caacucuggg gguuaacaag      180

ugucacgugc agauuaugga cuugggacau augugcgaug caacgauguc cuaugagugu      240

cccauguuag acgaaggcgu agagccugau gacguggauu gcugguguaa uaccacaucu      300

acuugggucg uguacgguac cugccaccau aaaaaggggg aagcccgacg uucgagaagg      360

gcuguuacac ugccaucaca cagcacccgg aaacuacaaa cucgcaguca gacauggcuc      420

gaguccagag aauauaccaa gcaucugauc aagguggaga acuggauuuu uaggaauccg      480

ggauucgccc uugucgcggu ggcaaucgcc uggcuguugg gcucuagcac gucccagaaa      540

guaauauacc ucguuaugau ccugcugauu gcucccgccu auucaauccg guguauuggc      600

gugaguaacc gcgacuuugu cgaggggaug agcggaggua cuugggugga ugugguccuc      660

gaacacggcg ggugcguuac cgugauggca caggacaagc cuacaguaga uaucgaguua      720

gugacuacca cagucucuaa uauggcugaa gugcgauccu acuguuacga ggccucaaua      780

agcgacaugg cgagugauuc uagaugccca acccaaggag aggccuaucu ggacaaacag      840

uccgacacgc aguacguuug uaagaggacu cuuguggauc ggggcugggg uaacgggugc      900

ggauuguucg gcaaaggcag ccuggucaca ugcgcaaagu uuaccuguuc gaagaaaaug      960

acugggaagu cuauucagcc cgaaaaucua gaguaucgua ucaugcucuc cgugcacgga     1020

ucacaacaua gugguaugau uggcuacgaa acagacgagg aucgcgcuaa aguagaaguu     1080

accccuaaca gcccaagagc cgaggcuacg cugggagggu ucggcucccu uggucuggac     1140

ugcgagccca ggaccggguu ggauuuuucu gaccucuauu accugacaau gaauaacaag     1200

cacugguuag ugcauaaaga augguuccac gauaucccuc ugccauggca cgccggcgca     1260

gacacuggaa ccccgcauug gaauaacaag gaggcccucg ucgaauuuaa agaugcucac     1320

gcgaagcggc agacaguggu cgugcuuggc agccaggagg gggcaguuca uacugcccug     1380

gcuggagccu uggaagcaga gauggacggu gccaagggcc gacuauucuc agggcaccug     1440

aaaugucgcc ucaagaugga uaaacugaga cuuaagggag ugaguuauuc ccugugcacc     1500

gcugccuuua cauucaccaa aguacccgca gagacuuugc auggcacggu cacaguggaa     1560

guucaguacg cggguaccga cgggccuugu aagaucccag ugcaaauggc ugucgacaug     1620

cagacucuca cccccguggg caggcugauu acagccaauc cuguaauaac cgagagcacu     1680

gaaaacucua agaugauguu agagcuggau ccacccuuug gagacucaua caucgugauu     1740

ggcguugggg auaaaaagau cacacaccac uggcaucgga guggaagcac gauagguaaa     1800

gcauucgaag ccaccguccg cggcgcuaag agaauggccg ugcuagggga cacugcaugg     1860

gauuucggau ccgugggcgg ugucuuuaau ucucucggga aaggcauuca ccagaucuuc     1920

ggagcugccu uuaagucgcu guucggcggg augagcuggu uuucccaaau ccuuauugga     1980

acauugcugg uuuggcucgg ccugaacacc aagaacgguu caaucagucu uacaugccug     2040

gcgcucgggg gagugaugau uuucuugucu acugccguga gcgcuugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10132
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10132
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcuguu accaggcggg guucagccua cuauauguac      120

cuugaccgca acgaugcagg cgaggccaua aguuucccca caacucuggg gaugaauaaa      180

uguuauaucc agauuaugga cuugggacac augugcgaug cuacgaugag cuacgaaugu      240

ccuauguuag acgagggcgu ggaaccagau gacguagauu gcugguguaa caccacaucc      300

acuugggugg ucuaugguac cugccaucac aagaaagggg aggcacgacg uucuagaagg      360

gccgugacac ugccgucgca uucaacccgg aagcuacaaa cucgcagcca gacauggcuc      420

gaaaguagag aguacaccaa acaccugauc aggguugaga auuggauuuu ucggaacccc      480

ggauucgcuc uugccgcggc agccaucgcu uggcuguugg gcuccucuac gagccagaag      540

gugauauauc ucgucaugau ccugcugauu gccccugcau acuccauccg cuguauuggc      600

gugucaaauc gagacuuugu agaagggaug aguggaggua cuuggguuga ugugguccuc      660

gagcauggcg ggugcgugac cgugauggcu caggacaagc caacagucga uaucgaauua      720

guuacuacca cagugagcaa cauggccgag guaagaucuu acuguuauga ggcguccaua      780

ucagacaugg ccagcgauag uaggugcccc acccaaggag aagcauaccu ggacaaacag      840

ucugacacgc aguaugugug uaagcggacu cuugucgauc guggcugggg uaaugggugc      900

ggauuguucg gcaaaggcuc ccuggugaca ugcgcuaagu uugccuguag caaaaagaug      960

accgggaagu cgauucagcc ugagaaccua gaauaccgca ucaugcucuc uguucacgga     1020

ucccaacacu cagguaugau ugugaaugac acuggccaug agacagauga aaacagagcu     1080

aaagucgaga ucaccccaaa uagucccagg gccgaggcaa cgcuuggggg auucggcagc     1140

cugggucugg acugcgaacc ucggaccggg uuggauuuuu ccgaccucua uuaccugaca     1200

augaacaaua agcacugguu agugcauaaa gagugguucc acgauauccc acugccgugg     1260

cacgccggcg cugacacugg aacaccccau uggaacaaua aggaagcgcu cguagaguuu     1320

aaagaugcac acgccaagcg acagaccguu gugguccuug gcucucagga aggggcugug     1380

cauacugccc uggcaggagc cuuggaggcu gagauggacg gugccaaagg ccgccuaagc     1440

ucagggcacc ugaaguguag acucaagaug gauaaacuga ggcuuaaggg agucaguuau     1500

ucccugugca ccgcagcguu cacauuuacc aaaauuccug cugaaacuuu gcauggcacg     1560

gugacaguug aggugcagua cgccgguacc gacgggccau guaagguacc cgcacaaaug     1620

gccgucgaca ugcagacucu caccccugug ggccggcuga uaacagcuaa cccaguuauc     1680

accgaaagca cugagaacuc uaaaaugaug uuagaacugg aucccccguu cggagacuca     1740

uacauuguga ucggcgucgg ggagaagaag auaacacacc acuggcaucg caguggaagc     1800

acgauuggua aagccuuuga ggcaaccgug agaggcgcua agaggauggc cguacuaggg     1860

gauacugcgu gggacuucgg auccgugggc ggugcccuca auucucuggg gaaaggcauc     1920

caccagaucu ucggagcugc auuuaagucg cuuuucggcg ggaugagcug guuuucccaa     1980

auuuugaucg gaacacugcu cauguggcug ggccuuaaca ccaaaaaugg uucaauuagu     2040

cugaugugcu uggcccucgg gggaguucug auauucuuau cuacagcugu cagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10133
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10133
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gugaccagaa ggggaucagc guacuauaug      120

uaccuugacc ggaacgaugc uggugaagcc aucaguuucc cuacaacucu gggcaugaau      180

aagugcuaua uucaaaucau ggacuugggg cacaugugug augcaacgau gagcuacgag      240

ugcccaaugu uagacgaagg aguagagccg gaugacgugg auuguuggug caacaccaca      300

uccacuuggg ucguguaugg caccugucau cacaaaaagg gugaagcccg ccgaucucgu      360

agagcuguua cacugcccuc gcauucaacc aggaaacuac agacucggag ccagacaugg      420

cucgagaguc gcgaguacac caagcaccug auuagagugg aaaauuggau cuuuaggaac      480

ccuggguucg cacuugccgc ugccgcgaua gcauggcugu ugggauccuc uacgagccag      540

aaagucaucu aucucgugau gauucugcug aucgccccag cuuacuccau ucggugcauc      600

ggcguaucaa aucgcgacuu uguugagggc augagugggg gaacuugggu ggauauaguc      660

cucgaacaug guggcugugu gaccgugaug gcccaagaca agcccacagu cgauauugag      720

uuaguuacua ccacagugag caacauggca gagguacgau cuuacugcua ugaggcuucc      780

aucucagaca uggccagcga uaguagaugu ccuacccagg gggaagcgua ccuggacaag      840

cagucugaca cgcaguaugu gugcaaaagg acucuugucg aucggggaug gggcaauggu      900

uguggguugu ucggaaaggg cucccuggug acaugcgcca aauuugcaug cagcaagaaa      960

augaccggca agucgauuca accagaaaac cuagaguacc guaucaugcu cucuguucac     1020

gggucccagc acucaggaau gaucgugaau gacacugguc augaaacaga ugagaaccgc     1080

gcuaaggucg agauuacccc caauaguccu agagccgaag cuacgcuggg cggguucgga     1140

agccuuggcc uggacuguga gccaaggacc gguuuggauu uuuccgaccu cuauuaccug     1200

acaaugaaca auaaacacug guuagugcau aaggaauggu uccacgauau accgcugccc     1260

uggcacgccg gggcagacac uggcaccccu cauuggaaca auaaagaggc ccucguagaa     1320

uuuaaggaug cucacgcgaa acggcagaca guuguggucc uuggaucuca ggagggcgca     1380

gugcauacug cccuggcugg ggccuuggag gcagaaaugg acggagccaa gggucgacua     1440

agcucaggcc accugaaaug ccgccucaag auggauaagc ugagacuuaa aggggucagu     1500

uauucccugu guaccgcugc cuucacauuu accaagaucc cagcagagac uuugcaugga     1560

acggugacag uugaagugca auacgcgggc accgacgguc ccugcaaagu accugcucag     1620

auggccgucg acaugcagac ucucacccca guggggaggc ugauuacagc aaaccccguu     1680

aucaccgaga gcacugaaaa cucuaagaug auguuagagc uggauccgcc uuucggcgac     1740

ucauacauag ugauuggagu cggcgagaaa aagaucacac accacuggca ucggaguggg     1800

agcacgcuag gaaaggccuu cuccaccacu cucaaaggug cucaacgccu ggccgcacuu     1860

ggcgauacag cuugggacuu ugggucuauc ggaggcgugu ucaauucgau ugguagagcc     1920

guacaccagg uguuuggggg cgcguucagg accuuguuug gaggcaugag cuggaucaca     1980

caggggcuga ugggagcccu ccugcuuugg augggcguua acgcucguga ucgguccauu     2040

gcacuggccu ucuuggcuac uggugggguc cucguguuuc uggccaccaa ugugcaugca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10134
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10134
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gugaccagaa ggggaucagc guacuauaug      120

uaccuugacc ggaacgaugc uggugaagcc aucaguuucc cuacaacucu gggcaugaau      180

aagugcuaua uucaaaucau ggacuugggg cacacgugug augcaaccau gagcuacgag      240

ugcccaaugu uagacgaagg aguagagccg gaugacgugg auuguuggug caacacaacu      300

uccaccuggg ucguguaugg cacaugucau cacaaaaagg gugaagcccg ccgaucucgu      360

agagcuguua cccugcccuc gcauucaacu aggaaacuac agacacggag ccagaccugg      420

cucgagaguc gcgaguacac gaagcaccug auuagagugg aaaauuggau cuuuaggaac      480

ccuggguucg cacuugccgc ugccgcgaua gcauggcugu ugggauccuc uacuagccag      540

aaagucaucu aucucgugau gauucugcug aucgccccag cuuacuccau ucggugcauc      600

ggcguaucaa aucgcgacuu uguugagggc augagugggg gaaccugggu ggaugucgug      660

cucgaacaug guggcugugu gacagucaug gcccaagaca agcccacugu ugauauagag      720

uuagugacca caaccguaag caacauggca gaggugcgau cuuacugcua ugaggcuucc      780

auuucagaca uggccagcga uaguagaugu ccuacgcagg gggaagcgua ccuggacaag      840

cagucugaca cucaguaugu cugcaaaagg acacuugugg aucggggaug gggcaauggu      900

uguggguugu ucggaaaggg cucccugguu accugcgcca aauuugcaug cagcaagaaa      960

augacuggca agucgaucca accagaaaac cuagaguacc guauuaugcu cucugugcac     1020

gggucccagc acucaggaau gaucgucaau gacacagguc augaaaccga ugagaaccgc     1080

gcuaaggugg agaucacgcc caauaguccu agagccgaag cuacccuggg cggguucgga     1140

agccuuggcc uggacuguga gccaaggaca gguuuggauu uuuccgaccu cuauuaccug     1200

acuaugaaca auaaacacug guuaguacau aaggaauggu uccacgauau uccgcugccc     1260

uggcacgccg gggcagacac cggcacaccu cauuggaaca auaaagaggc ccucguugaa     1320

uuuaaggaug cucacgcgaa acggcagacu guggucgugc uuggaucuca ggagggcgca     1380

guccauaccg cccuggcugg ggccuuggag gcagaaaugg acggagccaa gggucgacua     1440

agcucaggcc accugaaaug ccgccucaag auggauaagc ugagacuuaa aggggugagu     1500

uauucccugu guacagcugc cuucaccuuu acuaagauac cagcagagac guugcaugga     1560

acaguuaccg uggaaguaca auacgcgggc acugacgguc ccugcaaagu cccugcucag     1620

auggccgugg acaugcagac ccucacacca guugggaggc ugaucaccgc aaaccccgug     1680

auuacugaga gcacagaaaa cucuaagaug auguuagagc uggauccgcc uuucggcgac     1740

ucauacaucg ucauaggagu gggcgagaaa aagauuacgc accacuggca ucggaguggg     1800

agcacccuag gaaaggccuu cuccacuaca cucaaaggug cucaacgccu ggccgcacuu     1860

ggcgauaccg cuugggacuu ugggucuauc ggaggcguau ucaauucgau cgguagagcc     1920

gugcaccagg uuuuuggggg cgcguucagg acauuguuug gaggcaugag cuggauuacu     1980

caggggcuga ugggagcccu ccugcuuugg augggcguca acgcucguga ucgguccauc     2040

gcacuggccu ucuuggcuac cgguggggug cucguguuuc uggccacgaa uguccaugca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10135
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10135
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc aucaccagaa ggggaucagc guacuauaug      120

uaccuugacc ggagugaugc ugguaaggcc auuagcuucg caacaacucu gggcgugaac      180

aaaugccacg uacaaaucau ggacuugggg cauaugugug augccacgau guccuaugaa      240

ugcccuaugu uagacgaggg aguggaacca gaugacgucg auuguuggug caauaccaca      300

ucuacuuggg ugguuuacgg caccugucac cauaagaaag gugaggcucg ccgaucgcgu      360

agagcaguga cacugccguc acacagcacc aggaagcuac agacucggag ucagacaugg      420

cucgaauccc gcgaguauac caaacaucug auuaaggucg agaacuggau cuuuagaaau      480

cccggguucg cccuuguggc uguagccaua gcguggcugu ugggaucuag cacgucccag      540

aagguuaucu accucgugau gauucugcug aucgcacccg ccuauucaau uaggugcauc      600

ggcgucagua accgggacuu uguggaaggc augagcgggg gaacuugggu ggaugucguu      660

cucgagcacg guggcugugu gaccguaaug gcucaagaca aaccaacagu ggauauagaa      720

uuagucacua ccacaguguc uaauauggcc gagguucgcu ccuacugcua cgaggcauca      780

auuagcgaca uggcuaguga uucucgaugu cccacccagg gggaagccua ucuggacaag      840

caguccgaca cgcaguacgu gugcaaaaga acucuugucg auaggggaug gggcaacggu      900

uguggguugu ucggaaaggg cagccuggug acaugcgcga aauuuaccug cucgaagaag      960

augacuggca aaucuaucca accugagaau cuagaauauc ggauuaugcu cuccguacac     1020

gggucacagc auaguggaau gaucgguuac gagacagacg aagaucgugc caagguugag     1080

gugaccccaa acagcccccg cgcagaggcu acgcugggcg gguucggauc ccuuggccug     1140

gacugugaac cuagaaccgg uuuggauuuu ucugaccucu auuaccugac aaugaauaac     1200

aaacacuggu uaguccauaa ggagugguuc cacgauaucc cacugccgug gcacgccggg     1260

gcugacacug gcaccccuca uuggaauaac aaagaagccc ucguggaguu uaaggaugca     1320

cacgccaaaa ggcagacagu cgugguucuu ggaagccagg aaggcgcugu gcauacugcg     1380

cuggcagggg ccuuggaggc ugagauggac ggagccaagg gucggcuauu cucaggccac     1440

cugaagugcc gacucaaaau ggauaagcug cgccuuaaag ggguaaguua uucccugugu     1500

accgcagccu uuacauucac caaggucccu gcugaaacuu ugcauggaac ggugacaguu     1560

gaggugcaau acgccggcac cgacggucca ugcaaaauuc ccguccagau ggcaguggac     1620

augcagacuc ucaccccugu agggagacug auaacagcga auccagugau caccgaaagc     1680

acugagaacu cuaagaugau guuagaacug gaucccccgu ucggcgacuc auacauuguu     1740

aucggagucg gcgauaagaa aauaacacac cacuggcaua ggagugggag cacgcuagga     1800

aaggcuuuuu ccaccacucu caaaggugcc caacggcugg cagcccuugg cgacacagcu     1860

ugggauuucg ggucuauugg aggcguguuu aauucgaucg gucgcgccgu gcaccagguc     1920

uucgggggcg cauuuagaac cuuguucgga ggcaugagcu ggaucacaca ggggcugaug     1980

ggagcucucc ugcuuuggau gggcguuaac gccagggacc guuccauugc gcuggccuuu     2040

uuggcuacug guggggugcu cguguuccug gcaaccaacg uacaugccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10136
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10136
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gugaccagaa ggggaucagc guacuauaug      120

uaccuugacc ggaacgaugc uggugaagcc aucaguuucc cuacaacucu gggcaugaau      180

aagugcuaua uucaaaucau ggacuugggg cacaugugug augcaacgau gagcuacgag      240

ugcccaaugu uagacgaagg aguagagccg gaugacgugg auuguuggug caacaccaca      300

uccacuuggg ucguguaugg caccugucau cacaaaaagg gugaagcccg ccgaucucgu      360

agagcuguua cacugcccuc gcauucaacc aggaaacuac agacucggag ccagacaugg      420

cucgagaguc gcgaguacac caagcaccug auuagagugg aaaauuggau cuuuaggaac      480

ccuggguucg cacuugccgc ugccgcgaua gcauggcugu ugggauccuc uacgagccag      540

aaagucaucu aucucgugau gauucugcug aucgccccag cuuacuccau ucggugcauc      600

ggcguaucaa aucgcgacuu uguugagggc augagugggg gaacuugggu ggaugucgug      660

cucgaacaug guggcugugu gaccgucaug gcccaagaca agcccacagu ugauauagag      720

uuagugacua ccacaguaag caacauggca gaggugcgau cuuacugcua ugaggcuucc      780

auuucagaca uggccagcga uaguagaugu ccuacccagg gggaagcgua ccuggacaag      840

cagucugaca cgcaguaugu cugcaaaagg acucuugugg aucggggaug gggcaauggu      900

uguggguugu ucggaaaggg cucccugguu acaugcgcca aauuugcaug cagcaagaaa      960

augaccggca agucgaucca accagaaaac cuagaguacc guauuaugcu cucugugcac     1020

gggucccagc acucaggaau gaucgucaau gacacugguc augaaacaga ugagaaccgc     1080

gcuaaggugg agaucacccc caauaguccu agagccgaag cuacgcuggg cggguucgga     1140

agccuuggcc uggacuguga gccaaggacc gguuuggauu uuuccgaccu cuauuaccug     1200

acaaugaaca auaaacacug guuaguacau aaggaauggu uccacgauau uccgcugccc     1260

uggcacgccg gggcagacac uggcaccccu cauuggaaca auaaagaggc ccucguugaa     1320

uuuaaggaug cucacgcgaa acggcagaca guggucgugc uuggaucuca ggagggcgca     1380

guccauacug cccuggcugg ggccuuggag gcagaaaugg acggagccaa gggucgacua     1440

agcucaggcc accugaaaug ccgccucaag auggauaagc ugagacuuaa aggggugagu     1500

uauucccugu guaccgcugc cuucacauuu accaagauac cagcagagac uuugcaugga     1560

acgguuacag uggaaguaca auacgcgggc accgacgguc ccugcaaagu cccugcucag     1620

auggccgugg acaugcagac ucucacccca guugggaggc ugaucacagc aaaccccgug     1680

auuaccgaga gcacugaaaa cucuaagaug auguuagagc uggauccgcc uuucggcgac     1740

ucauacaucg ucauaggagu gggcgagaaa aagauuacac accacuggca ucggaguggg     1800

agcacgcuag gaaaggccuu cuccaccacu cucaaaggug cucaacgccu ggccgcacuu     1860

ggcgauacag cuugggacuu ugggucuauc ggaggcguau ucaauucgau cgguagagcc     1920

gugcaccagg uuuuuggggg cgcguucagg accuuguuug gaggcaugag cuggauuaca     1980

caggggcuga ugggagcccu ccugcuuugg augggcguca acgcucguga ucgguccauc     2040

gcacuggccu ucuuggcuac ugguggggug cucguguuuc uggccaccaa uguccaugca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10137
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10137
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagcg uuacuaggcg gggcuccgcc uacuauaugu accuugaucg caacgacgcg      120

ggggaggcua uuucuuuucc cacgacccug ggaaugaaua agugcuauau acagaucaug      180

gauuuggguc auauguguga cgccacaaug ucauacgaau gcccuauguu agaugagggc      240

guggaaccag acgauguaga cuguuggugc aacacuacca guacaugggu ggucuauggg      300

accugucacc auaaaaaggg agaggcacga cguagcagaa gggccgugac ucugccgucc      360

cacucuacac ggaaacuaca aacccgcucg cagacguggc ucgaaucaag agaguacacu      420

aagcaucuga uuaggguuga gaauuggauc uuccggaacc ccggcuuugc ucuugcagcc      480

gcugccauug cguggcuguu ggguagcagu accucccaga aagugaucua ucucgucaug      540

auacugcuga ucgcacccgc cuacucuauu cgcugcaucg gggugagcaa ucgagauuuc      600

guagaaggaa uguccggcgg cacauggguu gacauugugc ucgagcacgg gggauguguc      660

acugugaugg cucaggauaa gccaaccgug gacaucgaau uagucacaac cacgguuuca      720

aacauggccg aggugagaag uuacugcuau gaggcaagca uaucugauau ggcuuccgac      780

ucaagguguc ccacucaagg ugaagccuac cuggacaagc agagcgauac acaguaugua      840

ugcaaacgga cccuugugga ccguggcugg gggaauggau guggcuuguu ugguaagggg      900

agucugguca cuugcgcgaa auucgccugc ucuaagaaaa ugacaggaaa guccauucag      960

ccugagaacc uagaauaccg caucaugcuc agcgugcacg gcucgcaaca uucuggcaug     1020

auuguuaaug auaccgggca cgagacggac gaaaacagag caaaggugga gaucacccca     1080

aauuccccca gggcugaggc cacacuggga gguuuuggcu cacuugggcu ggauugugaa     1140

ccucggacug gauuggacuu cagugaucuc uauuaccuga ccaugaacaa uaaacauugg     1200

uuaguccaca aggagugguu ucacgacauc ccacugccgu ggcaugcugg cgccgauaca     1260

gguacucccc acuggaacaa uaaagaagca cucguggagu ucaaggacgc ccaugcuaaa     1320

cgacagaccg uaguugugcu ugggagccag gaaggcgcgg uccacacagc acuggccgga     1380

gcuuuggagg ccgagaugga uggcgcaaag gggcgccuau ccucuggaca ucugaaaugc     1440

agacucaaga uggacaagcu gaggcuuaaa ggugugagcu auucacugug uaccgccgcu     1500

uuuacuuuca cgaagauucc ugccgaaaca uugcacggca ccgucacugu ggagguucag     1560

uacgcaggga ccgacggacc augcaaagug cccgcgcaaa uggcuguaga uaugcagaca     1620

cucaccccug ucggccggcu gauaacugcc aacccaguga ucacagaaag uacggagaac     1680

uccaagauga uguuagaacu ggacccuccg uuuggugaua gcuacauugu uaucggggug     1740

ggcgagaaaa agauaaccca ccauuggcac cgcucuggau caacucuagg caaggcauuc     1800

aguacaaccc ucaaaggggc ccagagacug gcugcccuug gagacacagc augggauuuc     1860

gguagcauug gcggggucuu uaauuccauc ggaagggcug ugcaucaagu auucggcggu     1920

gccuuucgua cuuuguucgg gggcaugucu uggaucaccc agggacugau gggcgcgcuc     1980

cugcuuugga ugggggugaa cgcccgggac cgaucgauug cucuggcauu uuuggccacg     2040

ggaggcguuc ucgucuuccu ggcuaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10138
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10138
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagcg uuacuaggcg gggcuccgcc uacuauaugu accuugaucg caacgacgcg      120

ggggaggcua uuucuuuucc cacgacccug ggaaugaaua agugcuauau acagaucaug      180

gauuuggguc auacauguga cgccacuaug ucauacgaau gcccuauguu agaugagggc      240

guggaaccag acgauguaga cuguuggugc aacaccacaa guaccugggu ggucuauggg      300

acuugucacc auaaaaaggg agaggcacga cguagcagaa gggccgugac acugccgucc      360

cacucuaccc ggaaacuaca aacgcgcucg cagacuuggc ucgaaucaag agaguacacc      420

aagcaucuga uuaggguuga gaauuggauc uuccggaacc ccggcuuugc ucuugcagcc      480

gcugccauug cguggcuguu ggguagcagu acaucccaga aagugaucua ucucgucaug      540

auacugcuga ucgcacccgc cuacucuauu cgcugcaucg gggugagcaa ucgagauuuc      600

guagaaggaa uguccggcgg cacuuggguu gacguggucc ucgagcacgg gggaugugug      660

accgugaugg cucaggauaa gccaacaguc gacauugaau uaguuaccac gacuguguca      720

aacauggccg agguaagaag uuacugcuau gaggcaagca ucucugauau ggcuuccgac      780

ucaagguguc ccacacaagg ugaagccuac cuggacaagc agagcgauac ccaguaugug      840

ugcaaacgga cucuugucga ccguggcugg gggaauggau guggcuuguu ugguaagggg      900

agucugguga caugcgcgaa auucgccugc ucuaagaaaa ugaccggaaa guccauacag      960

ccugagaacc uagaauaccg cauuaugcuc agcguucacg gcucgcaaca uucuggcaug     1020

aucgugaaug auacggggca cgagaccgac gaaaacagag caaaggucga gauuacacca     1080

aauuccccca gggcugaggc cacucuggga gguuuuggcu cacuugggcu ggauugugaa     1140

ccucggaccg gauuggacuu cagugaucuc uauuaccuga caaugaacaa uaaacauugg     1200

uuagugcaca aggagugguu ucacgacauc ccacugccgu ggcaugcugg cgccgauacu     1260

gguaccccuc acuggaacaa uaaagaagca cucguagagu ucaaggacgc ccaugcuaaa     1320

cgacagacag uugugguccu ugggagccag gaaggcgcgg ugcacaccgc acuggccgga     1380

gcuuuggagg ccgagaugga uggcgcaaag gggcgccuau ccucuggaca ucugaaaugc     1440

agacucaaga uggacaagcu gaggcuuaaa ggugucagcu auucacugug uacugccgcu     1500

uuuacguuca caaagauccc ugccgaaacc uugcacggca cugugaccgu ugaggugcag     1560

uacgcaggga cagacggacc augcaaagua cccgcgcaaa uggcugucga uaugcagacc     1620

cucacuccug ugggccggcu gauuacagcc aacccaguua uaacggaaag uaccgagaac     1680

uccaagauga uguuagaacu ggaucccccg uuuggugaca gcuacaucgu gauugggguc     1740

ggcgagaaaa agaucacuca ccauuggcac cgcucuggau caacacuagg caaggcauuc     1800

aguaccacac ucaaaggggc ccagagacug gcugcccuug gagacacugc augggauuuc     1860

gguagcauag gcgggguguu uaauuccauu ggaagggcug uacaucaagu guucggcggu     1920

gccuuucgua ccuuguucgg gggcaugucu uggaucacgc agggacugau gggcgcgcuc     1980

cugcuuugga uggggguuaa cgcccgggac cgaucgaucg cucuggcauu uuuggccacc     2040

ggaggcgucc ucguguuccu ggcuacaaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10139
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10139
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagca uuacuaggcg gggcuccgcc uacuauaugu accuugaucg cucugacgcg      120

gggaaggcua uaucauuugc cacgacccug ggaguuaaca aaugccaugu gcagaucaug      180

gauuuggguc acauguguga cgcaacaaug aguuaugagu gccccauguu agaugaaggc      240

guagagccug acgaugugga cuguuggugc aauacuacca gcacaugggu cguguacggg      300

accugucauc acaagaaagg agaagcccga cguuccagaa gggcuguuac ucugccaucu      360

cauucgacac ggaagcuaca aacccgcuca cagacguggc ucgagagcag agaauauacu      420

aaacaccuga uuaaggugga gaacuggauc uucaggaauc cgggcuuugc acuugucgcc      480

guggcuauug ccuggcuguu ggguaguucc accucucaga agguaaucua ccucguuaug      540

auacugcuga ucgcgcccgc auauagcauu cggugcaucg ggguguccaa ccgcgauuuc      600

gucgagggaa ugucaggcgg cacaugggug gacguggucc ucgaacacgg gggauguguu      660

acugugaugg cccaggauaa accuaccgua gacauugagu uagugacaac cacggucagu      720

aauauggcug aagugcgaag cuacugcuac gaggccucua ucuccgauau ggcaucagac      780

agcagauguc caacucaagg ugaggcuuau cuggacaagc agagugauac acaguacguu      840

ugcaaaagga cccuugugga ccggggcugg gggaacggau guggcuuguu ugguaagggg      900

ucucugguca cuugcgccaa auucacaugc uccaagaaga ugaccggaaa aagcauacag      960

cccgaaaauc uagaguaucg uauuaugcuc ucggugcaug gcucucaaca cuccggcaug     1020

aucggguacg aaacggauga ggaccgcgcg aagguagaag uuaccccuaa cucaccaaga     1080

gccgaggcaa cacugggagg uuuuggcagu cuugggcugg auugugagcc caggacugga     1140

uuggacuuca gcgaucucua uuaccugacc augaauaaca aacauugguu agugcacaag     1200

gaaugguuuc acgacauucc ucugccaugg caugcuggcg ccgauacagg uacuccgcac     1260

uggaauaaca aagaggcucu cgucgaguuc aaggacgccc augcaaaacg gcagaccgug     1320

gucgugcuug ggucccagga aggcgccguu cacacagcuc uggcgggagc auuggaagcc     1380

gagauggaug gcgcuaaggg gcgacuauuu ucuggacauc ugaagugccg ccucaaaaug     1440

gacaagcuga gacuuaaagg ugugagcuau ucacugugua ccgccgcauu cacuuuuacg     1500

aagguacccg ccgagacauu gcacggcacc gucacugugg aaguucagua cgcugggacc     1560

gacggaccuu gcaaaauccc agugcaaaug gccgucgaua ugcagacacu cacccccgug     1620

ggcaggcuga ucacugcaaa uccuguaauu acagagagua cggaaaacuc caagaugaug     1680

uuagagcugg acccacccuu cggugauagc uacauaguga ucgggguugg cgacaagaaa     1740

auuacccacc auuggcaccg gucuggauca acucuaggca aggcguucag uacaacccuc     1800

aaaggggcuc agcgccuggc cgcacuugga gauacagccu gggacuuugg uagcaucggc     1860

ggggucuuca auuccauagg cagagcugug caucaagugu uuggaggugc cuucaggacu     1920

uuguuugggg gcaugucuug gauuacccag ggacugaugg gcgcacuccu gcuuuggaug     1980

ggggucaacg cucgugaucg gucgaucgcc cuggcguucu uggccacggg aggcguucuc     2040

guguuucugg cuaccaacgu gcacgcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10140
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10140
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagcg uuacuaggcg gggcuccgcc uacuauaugu accuugaucg caacgacgcg      120

ggggaggcua uuucuuuucc cacgacccug ggaaugaaua agugcuauau acagaucaug      180

gauuuggguc auauguguga cgccacaaug ucauacgaau gcccuauguu agaugagggc      240

guggaaccag acgauguaga cuguuggugc aacacuacca guacaugggu ggucuauggg      300

accugucacc auaaaaaggg agaggcacga cguagcagaa gggccgugac ucugccgucc      360

cacucuacac ggaaacuaca aacccgcucg cagacguggc ucgaaucaag agaguacacu      420

aagcaucuga uuaggguuga gaauuggauc uuccggaacc ccggcuuugc ucuugcagcc      480

gcugccauug cguggcuguu ggguagcagu accucccaga aagugaucua ucucgucaug      540

auacugcuga ucgcacccgc cuacucuauu cgcugcaucg gggugagcaa ucgagauuuc      600

guagaaggaa uguccggcgg cacauggguu gacguggucc ucgagcacgg gggaugugug      660

acugugaugg cucaggauaa gccaaccguc gacauugaau uaguuacaac cacgguguca      720

aacauggccg agguaagaag uuacugcuau gaggcaagca ucucugauau ggcuuccgac      780

ucaagguguc ccacucaagg ugaagccuac cuggacaagc agagcgauac acaguaugug      840

ugcaaacgga cccuugucga ccguggcugg gggaauggau guggcuuguu ugguaagggg      900

agucugguga cuugcgcgaa auucgccugc ucuaagaaaa ugacaggaaa guccauacag      960

ccugagaacc uagaauaccg cauuaugcuc agcguucacg gcucgcaaca uucuggcaug     1020

aucgugaaug auaccgggca cgagacggac gaaaacagag caaaggucga gauuacccca     1080

aauuccccca gggcugaggc cacacuggga gguuuuggcu cacuugggcu ggauugugaa     1140

ccucggacug gauuggacuu cagugaucuc uauuaccuga ccaugaacaa uaaacauugg     1200

uuagugcaca aggagugguu ucacgacauc ccacugccgu ggcaugcugg cgccgauaca     1260

gguacucccc acuggaacaa uaaagaagca cucguagagu ucaaggacgc ccaugcuaaa     1320

cgacagaccg uugugguccu ugggagccag gaaggcgcgg ugcacacagc acuggccgga     1380

gcuuuggagg ccgagaugga uggcgcaaag gggcgccuau ccucuggaca ucugaaaugc     1440

agacucaaga uggacaagcu gaggcuuaaa ggugucagcu auucacugug uaccgccgcu     1500

uuuacuuuca cgaagauccc ugccgaaaca uugcacggca ccgugacugu ugaggugcag     1560

uacgcaggga ccgacggacc augcaaagua cccgcgcaaa uggcugucga uaugcagaca     1620

cucaccccug ugggccggcu gauuacugcc aacccaguua uaacagaaag uacggagaac     1680

uccaagauga uguuagaacu ggacccuccg uuuggugaua gcuacaucgu gauugggguc     1740

ggcgagaaaa agaucaccca ccauuggcac cgcucuggau caacucuagg caaggcauuc     1800

aguacaaccc ucaaaggggc ccagagacug gcugcccuug gagacacagc augggauuuc     1860

gguagcauag gcgggguguu uaauuccauu ggaagggcug uacaucaagu guucggcggu     1920

gccuuucgua cuuuguucgg gggcaugucu uggaucaccc agggacugau gggcgcgcuc     1980

cugcuuugga uggggguuaa cgcccgggac cgaucgaucg cucuggcauu uuuggccacg     2040

ggaggcgucc ucguguuccu ggcuaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10141
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10141
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcuguu accaggcggg guucagccua cuauauguac      120

cuugaccgca acgaugcagg cgaggccaua aguuucccca caacucuggg gaugaauaaa      180

uguuauaucc agauuaugga cuugggacac augugcgaug cuacgaugag cuacgaaugu      240

ccuauguuag acgagggcgu ggaaccagau gacguagauu gcugguguaa caccacaucc      300

acuugggugg ucuaugguac cugccaucac aagaaagggg aggcacgacg uucuagaagg      360

gccgugacac ugccgucgca uucaacccgg aagcuacaaa cucgcagcca gacauggcuc      420

gaaaguagag aguacaccaa acaccugauc aggguugaga auuggauuuu ucggaacccc      480

ggauucgcuc uugccgcggc agccaucgcu uggcuguugg gcuccucuac gagccagaag      540

gugauauauc ucgucaugau ccugcugauu gccccugcau acuccauccg cuguauuggc      600

gugucaaauc gagacuuugu agaagggaug aguggaggua cuuggguuga uaucgugcuc      660

gagcauggcg ggugcgucac cgugauggcu caggacaagc caacagugga uauagaauua      720

gucacuacca caguuagcaa cauggccgag gugagaucuu acuguuauga ggcguccauu      780

ucagacaugg ccagcgauag uaggugcccc acccaaggag aagcauaccu ggacaaacag      840

ucugacacgc aguauguaug uaagcggacu cuuguggauc guggcugggg uaaugggugc      900

ggauuguucg gcaaaggcuc ccuggucaca ugcgcuaagu uugccuguag caaaaagaug      960

accgggaagu cgauccagcc ugagaaccua gaauaccgca uuaugcucuc ugugcacgga     1020

ucccaacacu cagguaugau cguuaaugac acuggccaug agacagauga aaacagagcu     1080

aaaguggaga ucaccccaaa uagucccagg gccgaggcaa cgcuuggggg auucggcagc     1140

cugggucugg acugcgaacc ucggaccggg uuggauuuuu ccgaccucua uuaccugaca     1200

augaacaaua agcacugguu aguccauaaa gagugguucc acgauauucc acugccgugg     1260

cacgccggcg cugacacugg aacaccccau uggaacaaua aggaagcgcu cguggaguuu     1320

aaagaugcac acgccaagcg acagaccgua guugugcuug gcucucagga aggggcuguc     1380

cauacugccc uggcaggagc cuuggaggcu gagauggacg gugccaaagg ccgccuaagc     1440

ucagggcacc ugaaguguag acucaagaug gauaaacuga ggcuuaaggg agugaguuau     1500

ucccugugca ccgcagcguu cacauuuacc aaaauaccug cugaaacuuu gcauggcacg     1560

gucacagugg agguucagua cgccgguacc gacgggccau guaaggugcc cgcacaaaug     1620

gccguagaca ugcagacucu caccccuguc ggccggcuga ucacagcuaa cccagugauu     1680

accgaaagca cugagaacuc uaaaaugaug uuagaacugg aucccccguu cggagacuca     1740

uacaucguua uaggcguggg ggagaagaag auuacacacc acuggcaucg caguggaagc     1800

acgcuaggua aagccuuuuc caccacucuc aagggcgcac agagacuggc ugcccuuggg     1860

gauacagcgu gggacuucgg aucuaucggc ggugucuuca auucgaucgg gagggccgug     1920

caccaaguau uuggcggagc uuuccguacc uuguuuggcg ggaugagcug gauuacacag     1980

ggacugaugg gcgcacuccu gcuuuggaug ggugugaacg cccgggaucg auccaucgcu     2040

cuggccuucu uggcaacugg gggaguucuc gucuuucugg cgaccaaugu gcaugccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10142
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10142
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcuguu accaggcggg guucagccua cuauauguac      120

cuugaccgca acgaugcagg cgaggccaua aguuucccca caacucuggg gaugaauaaa      180

uguuauaucc agauuaugga cuugggacac acgugcgaug cuaccaugag cuacgaaugu      240

ccuauguuag acgagggcgu ggaaccagau gacguagauu gcugguguaa cacaacuucc      300

accugggugg ucuaugguac augccaucac aagaaagggg aggcacgacg uucuagaagg      360

gccgugaccc ugccgucgca uucaacucgg aagcuacaaa cacgcagcca gaccuggcuc      420

gaaaguagag aguacacgaa acaccugauc aggguugaga auuggauuuu ucggaacccc      480

ggauucgcuc uugccgcggc agccaucgcu uggcuguugg gcuccucuac uagccagaag      540

gugauauauc ucgucaugau ccugcugauu gccccugcau acuccauccg cuguauuggc      600

gugucaaauc gagacuuugu agaagggaug aguggaggua ccuggguuga ugugguccuc      660

gagcauggcg ggugcgugac agugauggcu caggacaagc caacugucga uaucgaauua      720

guuaccacaa ccgugagcaa cauggccgag guaagaucuu acuguuauga ggcguccaua      780

ucagacaugg ccagcgauag uaggugcccc acgcaaggag aagcauaccu ggacaaacag      840

ucugacacuc aguaugugug uaagcggaca cuugucgauc guggcugggg uaaugggugc      900

ggauuguucg gcaaaggcuc ccuggugacc ugcgcuaagu uugccuguag caaaaagaug      960

acugggaagu cgauucagcc ugagaaccua gaauaccgca ucaugcucuc uguucacgga     1020

ucccaacacu cagguaugau ugugaaugac acaggccaug agaccgauga aaacagagcu     1080

aaagucgaga ucacgccaaa uagucccagg gccgaggcaa cccuuggggg auucggcagc     1140

cugggucugg acugcgaacc ucggacaggg uuggauuuuu ccgaccucua uuaccugacu     1200

augaacaaua agcacugguu agugcauaaa gagugguucc acgauauccc acugccgugg     1260

cacgccggcg cugacaccgg aacaccccau uggaacaaua aggaagcgcu cguagaguuu     1320

aaagaugcac acgccaagcg acagacuguu gugguccuug gcucucagga aggggcugug     1380

cauaccgccc uggcaggagc cuuggaggcu gagauggacg gugccaaagg ccgccuaagc     1440

ucagggcacc ugaaguguag acucaagaug gauaaacuga ggcuuaaggg agucaguuau     1500

ucccugugca cagcagcguu caccuuuacu aaaauuccug cugaaacguu gcauggcaca     1560

gugaccguug aggugcagua cgccgguacu gacgggccau guaagguacc cgcacaaaug     1620

gccgucgaca ugcagacccu cacaccugug ggccggcuga uaaccgcuaa cccaguuauc     1680

acugaaagca cagagaacuc uaaaaugaug uuagaacugg aucccccguu cggagacuca     1740

uacauuguga ucggcgucgg ggagaagaag auaacgcacc acuggcaucg caguggaagc     1800

acccuaggua aagccuuuuc cacuacacuc aagggcgcac agagacuggc ugcccuuggg     1860

gauaccgcgu gggacuucgg aucuauuggc gguguguuca auucgaucgg gagggccgua     1920

caccaagugu uuggcggagc uuuccguaca uuguuuggcg ggaugagcug gaucacucag     1980

ggacugaugg gcgcacuccu gcuuuggaug gguguuaacg cccgggaucg auccauugcu     2040

cuggccuucu uggcaaccgg gggaguccuc guguuucugg cgacgaaugu gcaugccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10143
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10143
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcuaua accaggcggg guucagccua cuauauguac      120

cuugaccgca gugaugcagg caaagccauc agcuucgcua caacucuggg gguuaacaag      180

ugucacgugc agauuaugga cuugggacau augugcgaug caacgauguc cuaugagugu      240

cccauguuag acgaaggcgu agagccugau gacguggauu gcugguguaa uaccacaucu      300

acuugggucg uguacgguac cugccaccau aaaaaggggg aagcccgacg uucgagaagg      360

gcuguuacac ugccaucaca cagcacccgg aaacuacaaa cucgcaguca gacauggcuc      420

gaguccagag aauauaccaa gcaucugauc aagguggaga acuggauuuu uaggaauccg      480

ggauucgccc uugucgcggu ggcaaucgcc uggcuguugg gcucuagcac gucccagaaa      540

guaauauacc ucguuaugau ccugcugauu gcucccgccu auucaauccg guguauuggc      600

gugaguaacc gcgacuuugu cgaggggaug agcggaggua cuugggugga ugugguccuc      660

gaacacggcg ggugcguuac cgugauggca caggacaagc cuacaguaga uaucgaguua      720

gugacuacca cagucucuaa uauggcugaa gugcgauccu acuguuacga ggccucaaua      780

agcgacaugg cgagugauuc uagaugccca acccaaggag aggccuaucu ggacaaacag      840

uccgacacgc aguacguuug uaagaggacu cuuguggauc ggggcugggg uaacgggugc      900

ggauuguucg gcaaaggcag ccuggucaca ugcgcaaagu uuaccuguuc gaagaaaaug      960

acugggaagu cuauucagcc cgaaaaucua gaguaucgua ucaugcucuc cgugcacgga     1020

ucacaacaua gugguaugau uggcuacgaa acagacgagg aucgcgcuaa aguagaaguu     1080

accccuaaca gcccaagagc cgaggcuacg cuugggggau ucggcucccu gggucuggac     1140

ugcgagccca ggaccggguu ggauuuuucu gaccucuauu accugacaau gaauaacaag     1200

cacugguuag ugcauaaaga augguuccac gauaucccuc ugccauggca cgccggcgca     1260

gacacuggaa ccccgcauug gaauaacaag gaggcccucg ucgaauuuaa agaugcucac     1320

gcgaagcggc agacaguggu cgugcuuggc agccaggagg gggcaguuca uacugcccug     1380

gcuggagccu uggaagcaga gauggacggu gccaagggcc gacuauucuc agggcaccug     1440

aaaugucgcc ucaagaugga uaaacugaga cuuaagggag ugaguuauuc ccugugcacc     1500

gcugccuuua cauucaccaa aguacccgca gagacuuugc auggcacggu cacaguggaa     1560

guucaguacg cggguaccga cgggccuugu aagaucccag ugcaaauggc ugucgacaug     1620

cagacucuca cccccguggg caggcugauu acagccaauc cuguaauaac cgagagcacu     1680

gaaaacucua agaugauguu agagcuggau ccacccuuug gagacucaua caucgugauu     1740

ggcguugggg auaaaaagau cacacaccac uggcaucgga guggaagcac gcuagguaaa     1800

gcauucucca ccacucucaa gggcgcccag cgccuggcug cccuugggga cacagcaugg     1860

gauuucggau cuauaggcgg ugucuuuaau ucgauuggga gagcugugca ccaaguguuc     1920

ggcggagccu uuaggaccuu guucggcggg augagcugga ucacacaggg acugaugggc     1980

gcgcuccugc uuuggauggg ugucaacgcc cgugaccggu ccaucgcucu ggcauuuuug     2040

gccacugggg gaguucucgu guuccuggcu accaacgugc augccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10144
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10144
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcuguu accaggcggg guucagccua cuauauguac      120

cuugaccgca acgaugcagg cgaggccaua aguuucccca caacucuggg gaugaauaaa      180

uguuauaucc agauuaugga cuugggacac augugcgaug cuacgaugag cuacgaaugu      240

ccuauguuag acgagggcgu ggaaccagau gacguagauu gcugguguaa caccacaucc      300

acuugggugg ucuaugguac cugccaucac aagaaagggg aggcacgacg uucuagaagg      360

gccgugacac ugccgucgca uucaacccgg aagcuacaaa cucgcagcca gacauggcuc      420

gaaaguagag aguacaccaa acaccugauc aggguugaga auuggauuuu ucggaacccc      480

ggauucgcuc uugccgcggc agccaucgcu uggcuguugg gcuccucuac gagccagaag      540

gugauauauc ucgucaugau ccugcugauu gccccugcau acuccauccg cuguauuggc      600

gugucaaauc gagacuuugu agaagggaug aguggaggua cuuggguuga ugugguccuc      660

gagcauggcg ggugcgugac cgugauggcu caggacaagc caacagucga uaucgaauua      720

guuacuacca cagugagcaa cauggccgag guaagaucuu acuguuauga ggcguccaua      780

ucagacaugg ccagcgauag uaggugcccc acccaaggag aagcauaccu ggacaaacag      840

ucugacacgc aguaugugug uaagcggacu cuugucgauc guggcugggg uaaugggugc      900

ggauuguucg gcaaaggcuc ccuggugaca ugcgcuaagu uugccuguag caaaaagaug      960

accgggaagu cgauucagcc ugagaaccua gaauaccgca ucaugcucuc uguucacgga     1020

ucccaacacu cagguaugau ugugaaugac acuggccaug agacagauga aaacagagcu     1080

aaagucgaga ucaccccaaa uagucccagg gccgaggcaa cgcuuggggg auucggcagc     1140

cugggucugg acugcgaacc ucggaccggg uuggauuuuu ccgaccucua uuaccugaca     1200

augaacaaua agcacugguu agugcauaaa gagugguucc acgauauccc acugccgugg     1260

cacgccggcg cugacacugg aacaccccau uggaacaaua aggaagcgcu cguagaguuu     1320

aaagaugcac acgccaagcg acagaccguu gugguccuug gcucucagga aggggcugug     1380

cauacugccc uggcaggagc cuuggaggcu gagauggacg gugccaaagg ccgccuaagc     1440

ucagggcacc ugaaguguag acucaagaug gauaaacuga ggcuuaaggg agucaguuau     1500

ucccugugca ccgcagcguu cacauuuacc aaaauuccug cugaaacuuu gcauggcacg     1560

gugacaguug aggugcagua cgccgguacc gacgggccau guaagguacc cgcacaaaug     1620

gccgucgaca ugcagacucu caccccugug ggccggcuga uaacagcuaa cccaguuauc     1680

accgaaagca cugagaacuc uaaaaugaug uuagaacugg aucccccguu cggagacuca     1740

uacauuguga ucggcgucgg ggagaagaag auaacacacc acuggcaucg caguggaagc     1800

acgcuaggua aagccuuuuc caccacucuc aagggcgcac agagacuggc ugcccuuggg     1860

gauacagcgu gggacuucgg aucuauuggc gguguguuca auucgaucgg gagggccgua     1920

caccaagugu uuggcggagc uuuccguacc uuguuuggcg ggaugagcug gaucacacag     1980

ggacugaugg gcgcacuccu gcuuuggaug gguguuaacg cccgggaucg auccauugcu     2040

cuggccuucu uggcaacugg gggaguccuc guguuucugg cgaccaaugu gcaugccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10145
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10145
gggagaaagc uuaccaugac cagcgugggc auugucgggc ugcuccuuac aacugccaug       60

gcugcaguua cgcugcccuc ccacucuacc agaaaguugc agacaagguc acaaacuugg      120

uuagagaguc gggaauacac caaacaucug auucgcgugg agaacuggau auuccgaaau      180

ccuggauuug cccuagcggc ugccgcaauc gccuggcucc uggguagcuc cacaucucag      240

aagguaauuu aucuugugau gauccuguug auugcuccag cauacucgau ccguugcaua      300

ggcgucucaa acagagacuu cguggaaggg augagcggag gcaccugggu ugauaucgug      360

cucgagcacg gugggugugu cacugugaug gcccaggaca aaccgacagu agauauugaa      420

cugguuacca cgacugugag uaauauggcu gaggucaggu ccuauugcua cgaggccucu      480

aucagcgaca uggcguccga uucacggugu cccacccagg gagaagcaua ucuggacaag      540

caaagugaua cacaguacgu gugcaaacgc acucucgugg acagaggcug gggcaacggg      600

uguggauuau uugguaaggg cagccugguc acaugcgcca aguucgcuug uucuaaaaag      660

augacaggga aauccauuca gccugagaau cuugaauaua ggaucauguu gucaguucau      720

ggaagccagc acaguggcau gauagugaac gauaccgguc augagacgga cgagaaucgg      780

gccaagguag aaauuacucc aaacucuccc cgcgcagagg cuacacuagg gggauuuggc      840

ucccugggcc ucgauugcga accucgaacc gggcuggacu ucagcgaucu uuacuaccug      900

acuaugaaua acaaacacug guuggugcac aaggaguggu uucaugacau cccacucccc      960

uggcacgccg gagcggacac agguaccccu cauuggaaua acaaggaagc ccuggucgag     1020

uucaaagaug cacacgcuaa gagacaaacg gugguugugu uaggcucgca ggagggggcc     1080

guccacaccg cucuggccgg agcacucgaa gccgagaugg acggcgcuaa agguaggcuu     1140

ucuuccgggc aucugaagug ucgguugaaa auggauaagc uacgucugaa aggcguguca     1200

uauagucucu gcacagcggc auuuacuuuc accaagauuc cagccgaaac acugcacgga     1260

acuguaaccg uugaggugca guacgcuggc acagacgggc cguguaaggu ccccgcccag     1320

auggcagugg auaugcaaac ccuuacuccu gucggacgcc ugaucacggc caauccagug     1380

aucacagaaa gcaccgagaa cuccaaaaug auguuggagc ucgauccccc uuuuggugac     1440

ucuuauauug uuauaggcgu gggggaaaag aaaaucacuc aucacuggca uagaagcgga     1500

ucaaccauug gcaaggcuuu cgaggccaca guaaggggug caaaacggau ggcgguccug     1560

ggggacaccg cuugggauuu uggcagugug ggaggcgccu uaaauucccu ggggaaggga     1620

auccaccaga uauucggugc agccuucaag agccuauuug gcgggauguc uugguucuca     1680

cagauucuca ucggaacucu gcuuaugugg uugggccuga acaccaaaaa cgguaguauc     1740

agccucaugu gccuggcucu ugggggcguu cugaucuuuu uguccacggc cgugucugca     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10146
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10146
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcaguua cgcugcccuc ccacucuacc agaaaguugc agacaagguc acaaacuugg      120

uuagagaguc gggaauacac caaacaucug auucgcgugg agaacuggau auuccgaaau      180

ccuggauuug cccuagcggc ugccgcaauc gccuggcucc uggguagcuc cacaucucag      240

aagguaauuu aucuugugau gauccuguug auugcuccag cauacucgau ccguugcaua      300

ggcgucucaa acagagacuu cguggaaggg augagcggag gcaccugggu ugaugugguc      360

cucgagcacg gugggugugu gacuguaaug gcccaggaca aaccgacagu ugauaucgaa      420

cuggugacca cgacugucag uaauauggcu gaggugaggu ccuauugcua cgaggccucu      480

auuagcgaca uggcguccga uucacggugu cccacccagg gagaagcaua ucuggacaag      540

caaagugaua cacaguacgu gugcaaacgc acucucgucg acagaggcug gggcaacggg      600

uguggauuau uugguaaggg cagccugguu accugcgcca aguucgcuug uucuaaaaag      660

augacaggga aauccaucca gccugagaau cuugaauaua ggauuauguu gucagugcau      720

ggaagccagc acaguggcau gaucguaaac gauaccgguc augagacgga cgagaaucgg      780

gccaaggugg aaauaacucc aaacucuccc cgcgcagagg cuacacuggg aggauuuggc      840

ucccuaggcc ucgauugcga accucgaacc gggcuggacu ucagcgaucu uuacuaccug      900

acuaugaaua acaaacacug guugguccac aaggaguggu uucaugacau uccacucccc      960

uggcacgccg gagcggacac agguaccccu cauuggaaua acaaggaagc ccugguggag     1020

uucaaagaug cacacgcuaa gagacaaacg guuguggucu uaggcucgca ggagggggcc     1080

gugcacaccg cucuggccgg agcacucgaa gccgagaugg acggcgcuaa agguaggcuu     1140

ucuuccgggc aucugaagug ucgguugaaa auggauaagc uacgucugaa aggcguauca     1200

uauagucucu gcacagcggc auuuacuuuc accaagaucc cagccgaaac acugcacgga     1260

acuguuaccg uggaggucca guacgcuggc acagacgggc cguguaaggu gcccgcccag     1320

auggcagucg auaugcaaac ccuuacuccu gugggacgcc ugauuacggc caauccaguu     1380

aucacagaaa gcaccgagaa cuccaaaaug auguuggagc ucgauccccc uuuuggugac     1440

ucuuauaucg ugauuggcgu aggggaaaag aaaauaacuc aucacuggca uagaagcgga     1500

ucaaccaucg gcaaggcuuu cgaggccaca gucaggggug caaaacggau ggcggugcug     1560

ggggacaccg cuugggauuu uggcaguguu ggaggcgccu uaaauucccu ggggaagggg     1620

auucaccaga ucuucggugc agccuucaag agccuauuug gcgggauguc uugguucuca     1680

cagauacuca uuggaacucu gcuuaugugg uugggccuga acgcuaaaaa cgguaguauc     1740

agccucaugu gccuggcccu ugggggcgug cugaucuuuu uguccacagc agucucugcu     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10147
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10147
gggagaaagc uuaccaugac cagcaucggc auuauagggc ugcuccuuac aacugccaug       60

gcugcaguga cgcugcccuc ccacucuacc agaaaguugc agacaagguc acaaacuugg      120

uuagagaguc gggaauacac caaacaucug aucaaggucg agaacuggau uuuccgcaau      180

ccuggauuug cccuaguugc gguggcuauc gccuggcucc uggguagcuc cacaucucag      240

aaaguaauuu aucuugugau gauccuguug auagcaccag ccuacucgau ccgaugcauu      300

ggcgucucaa accgugacuu cguggaaggg augagcggag gcaccugggu ugaugugguc      360

cucgagcacg gugggugugu gacuguaaug gcucaggaca agccgacagu ugauaucgaa      420

cuggugacca cgacugucag uaauauggca gaggugagau ccuauugcua cgaggccucu      480

auuagcgaca uggcuuccga uucaaggugu cccacccagg gagaagccua ucuggacaaa      540

caaagugaua cacaguacgu gugcaagcgg acucucgucg accgcggcug gggcaacggg      600

uguggauuau uugguaaggg cagccugguu accugcgcga aauucacaug uucuaagaaa      660

augaccggga aguccaucca gccugagaau cuugaauaua gaauaauguu gucagugcau      720

ggaagccagc acaguggcau gauugguuac gagacggaug aggacagggc aaaaguagaa      780

gugacuccaa acucuccccg ggccgaggcu acacuugggg gauuuggcuc ccuaggccuc      840

gauugcgaac cucgcaccgg gcuggacuuc agcgaucuuu acuaucugac uaugaauaac      900

aagcauuggu ugguccacaa ggagugguuu cacgacaucc cacuccccug gcaugccgga      960

gcagacacag guaccccuca cuggaauaac aaagaagcuc ugguggaguu caaggaugcc     1020

caugcgaaac gacaaacggu uguggucuua ggcucgcagg agggggccgu gcacaccgca     1080

cuggcuggag cccucgaagc ugagauggac ggcgccaagg guagacuguu uucugggcac     1140

cugaaaugua gguugaagau ggauaaacua cggcugaagg gcguauccua cucacucugc     1200

acagcagccu ucacuuuuac caagguucca gcugaaacac ugcauggaac ugugaccguc     1260

gaggugcagu augcgggcac agacgggccg uguaaaauuc ccguccagau ggcaguggau     1320

augcaaaccc uuacuccugu uggacgucug aucacggcca auccagugau cacagaaagu     1380

accgagaaca gcaagaugau guuggagcuc gaucccccuu ucggugacuc cuacauugua     1440

auaggcgucg gggacaaaaa gaucacucac cauuggcacc gcucuggaag caccauuggc     1500

aaggcuuuug aagccacagu gagaggugca aaaaggaugg ccguucuggg ggauaccgcu     1560

ugggacuucg gcucaguggg aggcgucuuc aauaguuuag ggaagggaau ccaucagaua     1620

uuuggugccg cauucaaauc ccuguuuggc gggaugagcu gguucucuca gauucuaauc     1680

ggaacucucc ugguguggcu uggcuugaac acaaagaaug guucaaucag ucugacgugc     1740

cucgcgcuug ggggcguaau gauuuuucug agcaccgcug uguccgccug aggacuaguu     1800

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     1860

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  10148
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10148
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccuuac aacugccaug       60

gcugcaguua cgcugcccuc ccacucuacc agaaaguugc agacaagguc acaaacuugg      120

uuagagaguc gggaauacac caaacaucug auucgcgugg agaacuggau auuccgaaau      180

ccuggauuug cccuagcggc ugccgcaauc gccuggcucc uggguagcuc cacaucucag      240

aagguaauuu aucuugugau gauccuguug auugcuccag cauacucgau ccguugcaua      300

ggcgucucaa acagagacuu cguggaaggg augagcggag gcaccugggu ugaugugguc      360

cucgagcacg gugggugugu gacuguaaug gcccaggaca aaccgacagu ugauaucgaa      420

cuggugacca cgacugucag uaauauggcu gaggugaggu ccuauugcua cgaggccucu      480

auuagcgaca uggcguccga uucacggugu cccacccagg gagaagcaua ucuggacaag      540

caaagugaua cacaguacgu gugcaaacgc acucucgucg acagaggcug gggcaacggg      600

uguggauuau uugguaaggg cagccugguu accugcgcca aguucgcuug uucuaaaaag      660

augacaggga aauccaucca gccugagaau cuugaauaua ggauuauguu gucagugcau      720

ggaagccagc acaguggcau gaucguaaac gauaccgguc augagacgga cgagaaucgg      780

gccaaggugg aaauaacucc aaacucuccc cgcgcagagg cuacacuggg aggauuuggc      840

ucccuaggcc ucgauugcga accucgaacc gggcuggacu ucagcgaucu uuacuaccug      900

acuaugaaua acaaacacug guugguccac aaggaguggu uucaugacau uccacucccc      960

uggcacgccg gagcggacac agguaccccu cauuggaaua acaaggaagc ccugguggag     1020

uucaaagaug cacacgcuaa gagacaaacg guuguggucu uaggcucgca ggagggggcc     1080

gugcacaccg cucuggccgg agcacucgaa gccgagaugg acggcgcuaa agguaggcuu     1140

ucuuccgggc aucugaagug ucgguugaaa auggauaagc uacgucugaa aggcguauca     1200

uauagucucu gcacagcggc auuuacuuuc accaagaucc cagccgaaac acugcacgga     1260

acuguuaccg uggaggucca guacgcuggc acagacgggc cguguaaggu gcccgcccag     1320

auggcagucg auaugcaaac ccuuacuccu gugggacgcc ugauuacggc caauccaguu     1380

aucacagaaa gcaccgagaa cuccaaaaug auguuggagc ucgauccccc uuuuggugac     1440

ucuuauaucg ugauuggcgu aggggaaaag aaaauaacuc aucacuggca uagaagcgga     1500

ucaaccaucg gcaaggcuuu cgaggccaca gucaggggug caaaacggau ggcggugcug     1560

ggggacaccg cuugggauuu uggcaguguu ggaggcgccu uaaauucccu ggggaagggg     1620

auucaccaga ucuucggugc agccuucaag agccuauuug gcgggauguc uugguucuca     1680

cagauacuca uuggaacucu gcuuaugugg uugggccuga acacgaaaaa cgguaguauc     1740

agccucaugu gccuggcucu ugggggcgug cugaucuuuu uguccacagc cgucucugca     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10149
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10149
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gcggugaccc uuccuucaca caguacaaga      120

aagcugcaaa cuaggagcca gacgugguug gaaucccggg aguacaccaa acauuuaauc      180

cgcguagaaa acuggauuuu ccgaaaucca ggauuugcuc uggccgcagc cgcuaucgca      240

uggcuacucg guucuucgac aucacagaag gugauuuauc uggucaugau ccuucugaua      300

gccccggcuu acagcauccg uugcauuggc gugaguaaca gagacuucgu ugaggggaug      360

uccggaggca cuugggugga uaucgucuug gaacacggug ggugugugac cguaauggcc      420

caggacaaac ccacaguuga uauugagcuc gugaccacua cagucucuaa uauggcggag      480

gugaggagcu auugcuacga agcauccauc ucagacaugg ccagugauag ccgguguccu      540

acccaaggag aggcuuaucu ggacaagcag ucugauacgc aguacgugug caaacgcacu      600

cuggucgaca gaggcugggg caacgggugu ggacucuuug guaagggcuc cuuaguuacc      660

ugcgccaagu ucgcauguag caaaaagaug acagggaaau caauacagcc agaaaaucug      720

gaguauagga uuaugcuuag ugugcaugga ucucaacacu ccggcaugau cguaaacgau      780

acuggucaug agaccgacga aaaucgggcu aagguggaga uuacacccaa cagcccucgc      840

gccgaagcga ccuugggagg auuuggcucg cugggccuag auugcgagcc acgaacgggg      900

cucgacuucu cugaucugua cuaccuuacu augaauaaca aacacuggcu gguccacaag      960

gaaugguuuc augacauccc cuugccuugg cacgccggag cagacacagg uaccccacau     1020

uggaauaaca aggaggcucu cguggaguuc aaagaugccc acgcuaagag acagacuguu     1080

gugguccugg gcucccagga aggggccgug cacacagcau uagccggagc ucuggaggcg     1140

gaaauggacg gcgcaaaagg uaggcucuca agugggcauc uuaagugucg gcugaaaaug     1200

gauaaguugc gucuaaaagg cguaagcuau ucccugugca ccgccgcuuu uacguucacc     1260

aagaucccgg ccgagacacu ccacggaacu guuaccgugg aaguccagua cgcaggcaca     1320

gacgggcccu guaaggugcc ugcccaaaug gcugucgaua ugcagacucu gaccccagug     1380

ggacgccuua uuacagccaa ucccguuaua accgagucua cugagaacag caaaaugaug     1440

cuggaauugg acccuccauu uggugauuca uauaucguga uuggcguagg ggagaagaaa     1500

aucacgcauc acuggcauag aaguggaucc acaauaggca aggcauucga agcgaccguc     1560

aggggugcua aacggauggc cgugcucggg gacacugcau gggauuucgg cagcguugga     1620

ggcgccuuaa auucucuggg gaaggggauu caccagaucu uuggugcugc cuucaaguca     1680

cuguuuggcg ggaugaguug guucagccaa auccuaauug gaacccuccu gauguggcuu     1740

ggcuugaaca ccaaaaacgg uuccaucucu cugaugugcc ucgcacuugg gggcgugcug     1800

auuuuucugu cgacagcugu cagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10150
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10150
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gcggugaccc uuccuucaca caguacaaga      120

aagcugcaaa cuaggagcca gacgugguug gaaucccggg aguacaccaa acauuuaauc      180

cgcguagaaa acuggauuuu ccgaaaucca ggauuugcuc uggccgcagc cgcuaucgca      240

uggcuacucg guucuucgac aucacagaag gugauuuauc uggucaugau ccuucugaua      300

gccccggcuu acagcauccg uugcauuggc gugaguaaca gagacuucgu ugaggggaug      360

uccggaggca cuugggugga ugucguguug gaacacggug gguguguaac cguuauggcc      420

caggacaaac ccacagugga uaucgagcuc gucaccacua cagugucuaa uauggcggag      480

gugaggagcu auugcuacga agcauccauu ucagacaugg ccagugauag ccgguguccu      540

acccaaggag aggcuuaucu ggacaagcag ucugauacgc aguacgucug caaacgcacu      600

cugguugaca gaggcugggg caacgggugu ggacucuuug guaagggcuc cuuagugacc      660

ugcgccaagu ucgcauguag caaaaagaug acagggaaau caauccagcc agaaaaucug      720

gaguauagga uaaugcuuag uguacaugga ucucaacacu ccggcaugau ugugaacgau      780

acuggucaug agaccgacga aaaucgggcu aaggucgaga ucacacccaa cagcccucgc      840

gccgaagcga ccuugggagg guuuggcucg cugggccuag auugcgagcc acgaacgggg      900

cucgacuucu cugaucugua cuaccuuacu augaauaaca aacacuggcu ggugcacaag      960

gaaugguuuc augacauucc cuugccuugg cacgccggag cagacacagg uaccccacau     1020

uggaauaaca aggaggcucu cguugaguuc aaagaugccc acgcuaagag acagacugug     1080

gucgugcugg gcucccagga aggggccgua cacacagcau uagccggagc ucuggaggcg     1140

gaaauggacg gcgcaaaagg uaggcucuca agugggcauc uuaagugucg gcugaaaaug     1200

gauaaguugc gucuaaaagg cguuagcuau ucccugugca ccgccgcuuu uacguucacc     1260

aagaucccgg ccgagacacu ccacggaacu gugaccgucg aagugcagua cgcaggcaca     1320

gacgggcccu guaagguccc ugcccaaaug gcuguggaua ugcagacucu gaccccaguu     1380

ggacgccuua ucacagccaa ucccgugauu accgagucua cugagaacag caaaaugaug     1440

cuggaauugg acccuccauu uggugauuca uauauaguaa ucggcgucgg ggagaagaaa     1500

auuacgcauc acuggcauag aaguggaucc acaaucggca aggcauucga agcgaccgug     1560

aggggugcua aacggauggc cguucucggg gacacugcau gggauuucgg cagcguggga     1620

ggcgccuuaa auucucuggg gaagggaaua caccagauuu uuggugcugc cuucaaguca     1680

cuguuuggcg ggaugaguug guucagccaa auccuaaucg gaacccuccu gauguggcuu     1740

ggcuugaacg cuaaaaacgg uuccauuucu cugaugugcc ucgcacuugg gggcguccug     1800

aucuuucugu cgacagccgu gagcgcguga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10151
<211>  2020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10151
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gcggugaccc uuccuucaca caguacaaga      120

aagcugcaaa cuaggagcca gacgugguug gaaucccggg aguacaccaa acauuuaauc      180

aagguagaaa acuggauuuu ccgcaaucca ggauuugcuc ugguggccgu cgcaaucgcc      240

uggcuacucg guucuucgac aucacagaaa gugauuuauc ugguuaugau ccuucugaua      300

gcuccggcau acagcauccg augcauuggc gugaguaacc gugacuucgu cgaggggaug      360

uccggaggca cuugggugga uguaguuuug gaacacggug ggugugugac cgucauggcc      420

caggacaagc ccacagugga uaucgagcuc gugaccacua cagucucuaa uauggcugag      480

guuagaagcu auugcuacga agccuccauu ucagacaugg cgagugauuc uagguguccu      540

acccaaggag aggcauaucu ggacaaacag agcgauacgc aguacgugug caagcggacu      600

cugguagacc gcggcugggg caacgggugu ggacucuuug guaagggcuc cuuagugacc      660

ugcgccaaau ucacauguuc aaagaaaaug acuggaaaga gcauccagcc agaaaaucug      720

gaguauagaa uaaugcuuag uguccauggg ucucaacacu ccggcaugau ugguuacgag      780

accgaugaag acagggcuaa aguggagguu acacccaaca gcccucgggc cgaagcaacc      840

uugggagggu uuggcucgcu gggccuagau ugcgagccac gcacggggcu cgacuucucu      900

gaucuguacu aucuuacuau gaauaacaag cauuggcugg ugcacaagga augguuucac      960

gacauccccu ugccuuggca ugcuggagcc gacacaggua ccccacacug gaauaacaaa     1020

gaggcgcucg ucgaguucaa ggaugcccau gcaaaacgac agacuguggu aguucugggc     1080

ucccaggaag gggcugugca cacagccuua gcuggagccc uggaggcaga aauggacggc     1140

gccaagggua gacucuuuuc agggcaccuu aaauguaggc ugaagaugga uaaauugcgg     1200

cuaaagggcg ucaguuacag ccugugcacc gcugcguuca cguuuaccaa ggugccggca     1260

gagacacucc auggaacugu caccguggaa guucaguaug ccggcacaga cgggcccugu     1320

aaaauuccug ugcaaauggc uguagauaug cagacucuga ccccagucgg acgucuuauc     1380

acagccaauc ccgugaucac cgaguccacu gagaacucua agaugaugcu ggaauuggac     1440

ccuccauucg gugauagcua cauuguuaua ggcguggggg acaaaaagau cacgcaccau     1500

uggcaccgcu caggaaguac aauuggcaaa gcauucgagg ccaccgucag aggugcuaag     1560

aggauggccg ugcucgggga uacugcaugg gacuuuggcu ccguaggagg cguguucaau     1620

agccugggga agggaaucca ucagauauuu ggugcggcuu ucaaaucuuu auuuggcggg     1680

augucauggu ucagucaaau ucugaucgga acccuacucg uuuggcuggg ccuuaacaca     1740

aagaauggua gcaucuccuu gaccugccug gcccucgggg gcgucaugau uuuucugucu     1800

acugcagugu cggccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     1860

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1980

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2020


<210>  10152
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10152
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuauagcu       60

guucucaugu ccgcacagga gucuugggcc gcggugaccc uuccuucaca caguacaaga      120

aagcugcaaa cuaggagcca gacgugguug gaaucccggg aguacaccaa acauuuaauc      180

cgcguagaaa acuggauuuu ccgaaaucca ggauuugcuc uggccgcagc cgcuaucgca      240

uggcuacucg guucuucgac aucacagaag gugauuuauc uggucaugau ccuucugaua      300

gccccggcuu acagcauccg uugcauuggc gugaguaaca gagacuucgu ugaggggaug      360

uccggaggca cuugggugga ugucguguug gaacacggug gguguguaac cguuauggcc      420

caggacaaac ccacagugga uaucgagcuc gucaccacua cagugucuaa uauggcggag      480

gugaggagcu auugcuacga agcauccauu ucagacaugg ccagugauag ccgguguccu      540

acccaaggag aggcuuaucu ggacaagcag ucugauacgc aguacgucug caaacgcacu      600

cugguugaca gaggcugggg caacgggugu ggacucuuug guaagggcuc cuuagugacc      660

ugcgccaagu ucgcauguag caaaaagaug acagggaaau caauccagcc agaaaaucug      720

gaguauagga uaaugcuuag uguacaugga ucucaacacu ccggcaugau ugugaacgau      780

acuggucaug agaccgacga aaaucgggcu aaggucgaga ucacacccaa cagcccucgc      840

gccgaagcga ccuugggagg guuuggcucg cugggccuag auugcgagcc acgaacgggg      900

cucgacuucu cugaucugua cuaccuuacu augaauaaca aacacuggcu ggugcacaag      960

gaaugguuuc augacauucc cuugccuugg cacgccggag cagacacagg uaccccacau     1020

uggaauaaca aggaggcucu cguugaguuc aaagaugccc acgcuaagag acagacugug     1080

gucgugcugg gcucccagga aggggccgua cacacagcau uagccggagc ucuggaggcg     1140

gaaauggacg gcgcaaaagg uaggcucuca agugggcauc uuaagugucg gcugaaaaug     1200

gauaaguugc gucuaaaagg cguuagcuau ucccugugca ccgccgcuuu uacguucacc     1260

aagaucccgg ccgagacacu ccacggaacu gugaccgucg aagugcagua cgcaggcaca     1320

gacgggcccu guaagguccc ugcccaaaug gcuguggaua ugcagacucu gaccccaguu     1380

ggacgccuua ucacagccaa ucccgugauu accgagucua cugagaacag caaaaugaug     1440

cuggaauugg acccuccauu uggugauuca uauauaguaa ucggcgucgg ggagaagaaa     1500

auuacgcauc acuggcauag aaguggaucc acaaucggca aggcauucga agcgaccgug     1560

aggggugcua aacggauggc cguucucggg gacacugcau gggauuucgg cagcguggga     1620

ggcgccuuaa auucucuggg gaagggaaua caccagauuu uuggugcugc cuucaaguca     1680

cuguuuggcg ggaugaguug guucagccaa auccuaaucg gaacccuccu gauguggcuu     1740

ggcuugaaca ccaaaaacgg uuccauuucu cugaugugcc ucgcacuugg gggcguccug     1800

aucuuucugu cgacagcugu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10153
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10153
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagcg ccguuacucu ucccucccau ucuacgagga agcugcagac ccggucacaa      120

acaugguugg agagucgcga auacacuaaa cacuuaauuc gaguggagaa cuggauauuu      180

cguaauccug gcuucgcgcu ggcugccgca gccaucgcuu ggcuacucgg gagcuccacc      240

ucucagaagg uaauuuaucu ggugaugauc cuucugauug caccagccua cucgaucaga      300

ugcauaggag ucucaaacag ggauuuugug gaagguauga gcggcgggac auggguugac      360

aucguguugg agcauggagg cugugucacc gugauggcuc aggauaaacc gacuguagac      420

auugaacucg uuacaaccac ggugaguaau auggccgagg uccgguccua uugcuacgag      480

gcgucuauca gcgauauggc auccgacuca cgcuguccca cucaggguga agccuaucug      540

gauaagcaaa gugacaccca guacgugugc aaaagaacac ugguggauag ggggugggga      600

aacggcugug gccucuucgg gaaggggagc uuagucacuu gcgcuaaguu ugccuguucu      660

aaaaagauga ccgguaaauc cauucagccu gagaaucugg aauaucggau caugcuuuca      720

guucacggca gccagcauag ugggaugaua gugaacgaca caggacacga gaccgaugag      780

aaucgcgcaa agguagaaau uacgccaaac ucuccccgag cugaggccac uuugggcggu      840

uucggguccc ugggacuaga cugcgaaccu agaacaggcc ucgauuuuag cgaccuguac      900

uaccuuacca ugaauaacaa acacuggcug gugcauaagg agugguucca cgacauccca      960

uugcccuggc augcgggcgc cgauacuggg acaccucacu ggaauaacaa ggaagcacuc     1020

gucgaguuua aagacgcuca cgccaagagg caaaccgugg uugugcuggg aucgcaggag     1080

ggugcugucc auacggccuu agcaggcgcc cuggaagcug agauggaugg ggcgaaagga     1140

cggcucucuu ccggccaccu uaagugucgu cugaaaaugg acaaguugcg ccuaaaaggu     1200

gugucauaua gucugugcac cgcagccuuc acauuuacua agauuccagc ugaaacccuc     1260

caugggacag uaacuguuga ggugcaguac gccggcaccg auggaccgug uaaggucccc     1320

gcacagaugg ccguggacau gcaaacacug accccugucg gcagacuuau cacugcuaau     1380

ccagugauca cggaaagcac agagaacucc aaaaugaugc uggaguugga ucccccuuuc     1440

ggggacucuu auauuguuau aggagugggu gaaaagaaaa ucacccacca uuggcacagg     1500

agcggcucaa cuauugggaa ggccuuugag gcaaccguac ggggagcgaa acgaauggcu     1560

guccucggcg auacagccug ggacuucggu agugugggag gcgcauuaaa uucccuggga     1620

aagggcaucc accagauauu cggggccgcu uuuaagagcc uguucggagg uaugucuugg     1680

uuuucacaga uucuaaucgg cacccuccug auguggcuug gguugaacac uaaaaacgga     1740

aguaucagcc ugaugugccu cgcccugggc gguguucuua uuuuccuguc cacagcagug     1800

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10154
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10154
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagcg ccguuacucu ucccucccau ucuacgagga agcugcagac ccggucacaa      120

acaugguugg agagucgcga auacacuaaa cacuuaauuc gaguggagaa cuggauauuu      180

cguaauccug gcuucgcgcu ggcugccgca gccaucgcuu ggcuacucgg gagcuccacc      240

ucucagaagg uaauuuaucu ggugaugauc cuucugauug caccagccua cucgaucaga      300

ugcauaggag ucucaaacag ggauuuugug gaagguauga gcggcgggac auggguugac      360

guggucuugg agcauggagg cugugugacc guaauggcuc aggauaaacc gacuguugac      420

aucgaacucg ugacaaccac ggucaguaau auggccgagg ugcgguccua uugcuacgag      480

gcgucuauua gcgauauggc auccgacuca cgcuguccca cucaggguga agccuaucug      540

gauaagcaaa gugacaccca guacgugugc aaaagaacac uggucgauag ggggugggga      600

aacggcugug gccucuucgg gaagggaagc cugguuacuu gcgcuaaguu ugccuguucu      660

aaaaagauga ccgguaaauc cauccagccu gagaaucugg aauaucggau uaugcuuuca      720

gugcacggca gccagcauag ugggaugauc guaaacgaca caggacacga gaccgaugag      780

aaucgcgcaa agguggaaau aacgccaaac ucuccccgag cugaggccac uuugggcggu      840

uucggguccc ugggacuaga cugcgaaccu agaacaggcc ucgauuuuag cgaccuguac      900

uaccuuacca ugaauaacaa acacuggcug guccauaagg agugguucca cgacauucca      960

uugcccuggc augcgggcgc cgauacuggg acaccucacu ggaauaacaa ggaagcacuc     1020

guggaguuua aagacgcuca cgccaagagg caaaccguug ugguccuggg aucgcaggag     1080

ggugcugugc auacggccuu agcaggcgcc cuggaagcug agauggaugg ggcgaaagga     1140

cggcucucuu ccggccaccu uaagugucgu cugaaaaugg acaaguugcg ccuaaaaggu     1200

guaucauaua gucugugcac cgcagccuuc acauuuacua agaucccagc ugaaacccuc     1260

caugggacag uuacugugga gguccaguac gccggcaccg auggaccgug uaaggugccc     1320

gcacagaugg ccgucgacau gcaaacacug accccugugg gcagacuuau uacugcuaau     1380

ccaguuauca cggaaagcac agagaacucc aaaaugaugc uggaguugga ucccccuuuc     1440

ggggacucuu auaucgugau uggaguaggu gaaaagaaaa uaacccacca uuggcacagg     1500

agcggcucaa cuaucgggaa ggccuuugag gcaaccgucc ggggagcgaa acgaauggcu     1560

gugcucggcg auacagccug ggacuucggu aguguugggg gcgcauuaaa uuccuuagga     1620

aagggcauuc accagaucuu cggggccgcu uuuaagagcc uguucggagg uaugucuugg     1680

uuuucacaga uacuaauugg cacccuccug auguggcuug gguugaacgc caaaaacgga     1740

aguaucagcc ugaugugccu cgcacugggc ggugugcuua ucuuccuguc cacugcuguc     1800

ucugccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10155
<211>  1999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10155
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagcg ccguuacucu ucccucccau ucuacgagga agcugcagac ccggucacaa      120

acaugguugg agagucgcga auacacuaaa cacuuaauua agguggagaa cuggauauuu      180

cgaaauccug gcuucgcgcu gguagcugug gccaucgcau ggcuacucgg gagcuccacc      240

ucucagaaag ucauuuaucu ggugaugauc cuucugauug ccccagcuua cucgauccgu      300

ugcauaggag uuucaaacag agauuuugug gaagguauga gcggcgggac augggucgac      360

gugguauugg agcauggagg cuguguuacc gugauggcac aggauaagcc gacugucgac      420

aucgaacucg ugacaaccac ggugaguaau auggccgagg ucagguccua uugcuacgag      480

gcuucuauua gcgauauggc cuccgacuca cgguguccca cucaggguga agcguaucug      540

gauaaacaaa gugacaccca guacguuugc aagcgcacac ugguggauag agggugggga      600

aacggcugug gccucuucgg gaaggggagc uuaguaacuu gcgcaaaauu uaccuguucu      660

aagaaaauga cagguaaguc cauccagccu gagaaucugg aauauaggau uaugcuuuca      720

gugcacggca gccagcauag ugggaugauc ggauacgaga ccgacgagga ucgggccaaa      780

gucgaaguga cgccaaacuc uccccgcgcu gaggccacuu ugggcgguuu cgggucccug      840

ggacuagacu gcgaaccucg aacaggccuc gauuuuagcg accuguacua ucuuaccaug      900

aauaacaagc acuggcuggu ucacaaggag ugguuccaug acauaccauu gcccuggcac      960

gcaggcgcug auacugggac accucauugg aauaacaaag aagcccucgu ggaguuuaag     1020

gacgcgcacg ccaaaagaca aaccgucgug guacugggau cgcaggaggg ugcaguucac     1080

acggcuuuag ccggcgcucu ggaagccgag auggaugggg caaagggaag gcuuuucucu     1140

ggccaucuca aaugucggcu gaagauggac aaauugcguc uaaagggugu guccuacuca     1200

cugugcaccg ccgcuuuuac auucacuaag gucccagcgg aaacccucca cgggacagug     1260

acugucgagg ugcaguaugc aggcaccgau ggaccgugua aaauucccgu ucagauggcc     1320

guggacaugc aaacacugac cccuguaggc cgccuuauca cugcuaaucc agucauuacg     1380

gaaaguacag agaacagcaa gaugaugcug gaguuggauc ccccuuuugg ggacuccuac     1440

aucgugaucg gaguugguga uaaaaagauu acccaucacu ggcauagauc uggcagcacu     1500

auagggaaag ccuucgaagc aaccgugagg ggagccaagc ggauggcugu ccucggcgac     1560

acagccuggg auuucgguuc agugggaggc guauuuaaua gucugggaaa gggcauccac     1620

cagauuuucg gggcagcguu uaaauccuua uucggaggua ugagcugguu uucucagauc     1680

cugauaggca cccuacucgu guggcugggg cuuaacacua agaauggauc aauuaguuug     1740

acaugccugg cucucggcgg uguuaugauc uuccugagca cggccgucuc cgcaugagga     1800

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     1860

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     1980

uuucagagcc accagaauu                                                  1999


<210>  10156
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10156
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugcaacaaga       60

guccacagcg ccguuacucu ucccucccau ucuacgagga agcugcagac ccggucacaa      120

acaugguugg agagucgcga auacacuaaa cacuuaauuc gaguggagaa cuggauauuu      180

cguaauccug gcuucgcgcu ggcugccgca gccaucgcuu ggcuacucgg gagcuccacc      240

ucucagaagg uaauuuaucu ggugaugauc cuucugauug caccagccua cucgaucaga      300

ugcauaggag ucucaaacag ggauuuugug gaagguauga gcggcgggac auggguugac      360

guggucuugg agcauggagg cugugugacc guaauggcuc aggauaaacc gacuguugac      420

aucgaacucg ugacaaccac ggucaguaau auggccgagg ugcgguccua uugcuacgag      480

gcgucuauua gcgauauggc auccgacuca cgcuguccca cucaggguga agccuaucug      540

gauaagcaaa gugacaccca guacgugugc aaaagaacac uggucgauag ggggugggga      600

aacggcugug gccucuucgg gaagggaagc cugguuacuu gcgcuaaguu ugccuguucu      660

aaaaagauga ccgguaaauc cauccagccu gagaaucugg aauaucggau uaugcuuuca      720

gugcacggca gccagcauag ugggaugauc guaaacgaca caggacacga gaccgaugag      780

aaucgcgcaa agguggaaau aacgccaaac ucuccccgag cugaggccac uuugggcggu      840

uucggguccc ugggacuaga cugcgaaccu agaacaggcc ucgauuuuag cgaccuguac      900

uaccuuacca ugaauaacaa acacuggcug guccauaagg agugguucca cgacauucca      960

uugcccuggc augcgggcgc cgauacuggg acaccucacu ggaauaacaa ggaagcacuc     1020

guggaguuua aagacgcuca cgccaagagg caaaccguug ugguccuggg aucgcaggag     1080

ggugcugugc auacggccuu agcaggcgcc cuggaagcug agauggaugg ggcgaaagga     1140

cggcucucuu ccggccaccu uaagugucgu cugaaaaugg acaaguugcg ccuaaaaggu     1200

guaucauaua gucugugcac cgcagccuuc acauuuacua agaucccagc ugaaacccuc     1260

caugggacag uuacugugga gguccaguac gccggcaccg auggaccgug uaaggugccc     1320

gcacagaugg ccgucgacau gcaaacacug accccugugg gcagacuuau uacugcuaau     1380

ccaguuauca cggaaagcac agagaacucc aaaaugaugc uggaguugga ucccccuuuc     1440

ggggacucuu auaucgugau uggaguaggu gaaaagaaaa uaacccacca uuggcacagg     1500

agcggcucaa cuaucgggaa ggccuuugag gcaaccgucc ggggagcgaa acgaauggcu     1560

gugcucggcg auacagccug ggacuucggu aguguugggg gcgcauuaaa uuccuuagga     1620

aagggcauuc accagaucuu cggggccgcu uuuaagagcc uguucggagg uaugucuugg     1680

uuuucacaga uacuaauugg cacccuccug auguggcuug gguugaacac uaaaaacgga     1740

aguaucagcc ugaugugccu cgcccugggc ggugugcuua ucuuccuguc cacagcaguc     1800

ucugcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10157
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10157
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcugcc guuacccuuc ccucacacag uacaaggaaa      120

cugcagacuc ggagccaaac gugguuggag ucccgcgaau acaccaagca uuuaauacga      180

guggagaacu ggaucuuccg uaauccuggu uuugcacugg ccgcugcagc cauugcuugg      240

cuacucgggu cuucgacauc acagaaagua aucuaucugg ugaugauucu ucugaucgcc      300

ccagcguaca gcauaagaug uaucgggguc aguaacaggg acuucgugga aggaaugucc      360

ggcgguacuu ggguugauau uguguuggag cacgggggau gcgucaccgu gauggcacag      420

gacaagccga caguagauau cgaacucguu accacuacag ugucuaauau ggccgagguc      480

cggagcuauu guuacgaggc uuccauuuca gacauggcca gugauagccg cugccccacc      540

cagggcgaag cauaucugga caaacaaucu gauacgcagu acguguguaa gagaacucug      600

guggacaggg gcugggggaa cggaugcggu cucuuuggca agggguccuu agucaccugu      660

gcuaaauucg ccugcucaaa gaaaaugaca ggaaagagca uccagccuga gaaucuggaa      720

uaucggauaa ugcuuagugu ucauggcucu cagcacuccg guaugauugu gaacgauacu      780

gggcaugaga ccgacgagaa ucgcgcgaaa guagaaauca caccaaacag cccccgagcc      840

gaggcaaccu ugggaggcuu uggcucgcug gggcuagauu gugaaccuag aacgggacuc      900

gacuucucug aucuguacua ccuuacuaug aauaacaagc acuggcuggu gcacaaggag      960

ugguuucaug acauuccauu gcccuggcac gcuggugccg acacaggcac cccucauugg     1020

aauaacaaag aagcucucgu cgaguucaag gaugcccacg caaaaaggca aacugugguu     1080

gugcuggggu cccaggaggg agccguccac acagcuuuag cgggcgcacu ggaagccgag     1140

auggacggug cuaaggggcg gcucucaagu ggccaucuua aaugccgucu gaagauggau     1200

aaauugcgcc uaaagggagu gagcuauucc cuguguaccg ccgcauuuac guucaccaag     1260

aucccagccg aaacacucca cggcacugua accguugagg ugcaguacgc ugggacagac     1320

ggaccgugca aaguccccgc ccagauggca guggauaugc aaacucugac cccugucggu     1380

agacuuauca cagcgaaucc agugauuacc gaaucuacug agaacagcaa gaugaugcug     1440

gaguuggauc ccccuuuugg cgacucauau auaguuaucg gggugggaga gaaaaagauu     1500

acgcaucacu ggcauaggag uggcuccaca aucgguaagg cuuucgaagc caccguacgg     1560

ggcgcaaaac gaauggccgu ccucggcgac acugcuuggg auuuuggaag cgugggcggg     1620

gccuuaaauu cucugggaaa ggguauacac cagauuuucg gcgcagcuuu caaaucacug     1680

uuugggggaa ugaguugguu cagccagauc cuaaucggca cccuccugau guggcuuggu     1740

uugaacacaa agaacggguc cauuucucug augugccucg cccugggcgg aguucuuauc     1800

uuucugucga ccgcggugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10158
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10158
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcugcc guuacccuuc ccucacacag uacaaggaaa      120

cugcagacuc ggagccaaac gugguuggag ucccgcgaau acaccaagca uuuaauacga      180

guggagaacu ggaucuuccg uaauccuggu uuugcacugg ccgcugcagc cauugcuugg      240

cuacucgggu cuucgacauc acagaaagua aucuaucugg ugaugauucu ucugaucgcc      300

ccagcguaca gcauaagaug uaucgggguc aguaacaggg acuucgugga aggaaugucc      360

ggcgguacuu ggguugaugu ggucuuggag cacgggggau gcgugaccgu aauggcacag      420

gacaagccga caguugauau ugaacucgug accacuacag ucucuaauau ggccgaggug      480

cggagcuauu guuacgaggc uuccaucuca gacauggcca gugauagccg cugccccacc      540

cagggcgaag cauaucugga caaacaaucu gauacgcagu acguguguaa gagaacucug      600

gucgacaggg gcugggggaa cggaugcggu cucuuuggca agggguccuu aguuaccugu      660

gcuaaauucg ccugcucaaa gaaaaugaca ggaaagagca uucagccuga gaaucuggaa      720

uaucggauca ugcuuagugu gcauggcucu cagcacuccg guaugauagu aaacgauacu      780

gggcaugaga ccgacgagaa ucgcgcgaaa guggaaauua caccaaacag cccccgagcc      840

gaggcaaccu ugggaggcuu uggcucgcug gggcuagauu gugaaccuag aacgggacuc      900

gacuucucug aucuguacua ccuuacuaug aauaacaagc acuggcuggu ccacaaggag      960

ugguuucaug acaucccauu gcccuggcac gcuggugccg acacaggcac cccucauugg     1020

aauaacaaag aagcucucgu ggaguucaag gaugcccacg caaaaaggca aacuguugug     1080

guccuggggu cccaggaggg agccgugcac acagcuuuag cgggcgcacu ggaagccgag     1140

auggacggug cuaaggggcg gcucucaagu ggccaucuua aaugccgucu gaagauggau     1200

aaauugcgcc uaaagggagu aagcuauucc cuguguaccg ccgcauuuac guucaccaag     1260

auuccagccg aaacacucca cggcacuguu accguggagg uccaguacgc ugggacagac     1320

ggaccgugca aagugcccgc ccagauggca gucgauaugc aaacucugac cccugugggu     1380

agacuuauca cagcgaaucc aguuaucacc gaaucuacug agaacagcaa gaugaugcug     1440

gaguuggauc ccccuuuugg cgacucauau auugugauag ggguaggaga gaaaaagauc     1500

acgcaucacu ggcauaggag uggcuccaca auugguaagg cuuucgaagc caccguccgg     1560

ggcgcaaaac gaauggccgu gcucggcgac acugcuuggg auuuuggaag cguuggcggg     1620

gccuuaaauu cucugggaaa ggguauccac cagauauucg gcgcagcuuu caaaucacug     1680

uuugggggaa ugaguugguu cagccagauu cuaaucggca cccuccugau guggcuuggu     1740

uugaacgcca agaacggguc caucucucug augugccucg cgcugggcgg agugcuuauu     1800

uuucugucga cagccgucag cgcuugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10159
<211>  2017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10159
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcugcc guuacccuuc ccucacacag uacaaggaaa      120

cugcagacuc ggagccaaac gugguuggag ucccgcgaau acaccaagca uuuaauaaaa      180

guggagaacu ggaucuuccg aaauccuggu uuugcacugg uagccguggc uauugcaugg      240

cuacucgggu cuucgacauc acagaagguc aucuaucugg ugaugauucu ucugaucgcc      300

ccagcuuaca gcauacguug uaucgggguu aguaacagag acuucgugga aggaaugucc      360

ggcgguacuu gggucgaugu gguauuggag cacgggggau gcguuaccgu gauggcccag      420

gacaaaccga cagucgauau ugaacucgug accacuacag ugucuaauau ggcggagguc      480

aggagcuauu guuacgaggc auccaucuca gacauggcca gugauagccg gugccccacc      540

cagggcgagg cuuaucugga caagcaaucu gauacgcagu acguuuguaa gcgcacucug      600

guggacagag gcugggggaa cggaugcggu cucuuuggca aaggguccuu aguaaccugu      660

gccaaguuca caugcagcaa aaagaugacu ggaaaaucaa uucagccuga aaaucuggaa      720

uauaggauca ugcuuagugu gcauggcucu cagcacuccg guaugauagg guacgagacc      780

gaugaggacc gggcaaaggu cgaagugaca ccaaacagcc cccgcgcuga ggccaccuug      840

ggaggcuuug gcucgcuggg gcuagauugu gaaccucgaa cgggacucga cuucucugau      900

cuguacuauc uuacuaugaa uaacaagcau uggcugguuc acaaagagug guuucacgac      960

auuccauugc ccuggcaugc gggugccgac acaggcaccc cucacuggaa uaacaaggaa     1020

gcacucgugg aguucaaaga ugcucaugcc aagagacaaa cugucguggu acuggggucc     1080

caggagggag cuguucacac agccuuagca ggcgcccugg aagcugagau ggacggugcg     1140

aaagggaggc ucuuuucagg ccaccuuaag ugccggcuga aaauggauaa guugcgucua     1200

aagggaguga guuacagccu guguaccgca gccuucacgu uuaccaaagu cccagcugaa     1260

acacuccaug gcacugugac cgucgaggug caguaugccg ggacagacgg accgugcaag     1320

auccccguuc agauggcagu ggauaugcaa acucugaccc cuguaggucg ccuuauuaca     1380

gccaauccag ucaucaccga auccacugag aacucuaaaa ugaugcugga guuggauccc     1440

ccuuucggcg acagcuacau cgugauuggg guuggagaca agaaaauaac gcaccauugg     1500

cacagaucag gcaguacaau cgguaaggcu uuugaagcca ccgugagggg cgcaaagcgg     1560

auggcggucc ucggcgauac ugcuugggac uucggauccg ugggcggggu auucaauagc     1620

cugggaaaag guauucauca gaucuuuggc gccgcauuca agucuuuauu ugggggaaug     1680

ucaugguuca gucagauacu gauuggcacc cuacucgugu ggcugggucu uaacaccaaa     1740

aaugggagca ucuccuugac augccuggcc cucggcggag uuaugaucuu ucugucuacu     1800

gcugucucgg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     1860

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1980

ccccccccca aaggcucuuu ucagagccac cagaauu                              2017


<210>  10160
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10160
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gcagugguca uugccugcgc gggagcugcc guuacccuuc ccucacacag uacaaggaaa      120

cugcagacuc ggagccaaac gugguuggag ucccgcgaau acaccaagca uuuaauacga      180

guggagaacu ggaucuuccg uaauccuggu uuugcacugg ccgcugcagc cauugcuugg      240

cuacucgggu cuucgacauc acagaaagua aucuaucugg ugaugauucu ucugaucgcc      300

ccagcguaca gcauaagaug uaucgggguc aguaacaggg acuucgugga aggaaugucc      360

ggcgguacuu ggguugaugu ggucuuggag cacgggggau gcgugaccgu aauggcacag      420

gacaagccga caguugauau ugaacucgug accacuacag ucucuaauau ggccgaggug      480

cggagcuauu guuacgaggc uuccaucuca gacauggcca gugauagccg cugccccacc      540

cagggcgaag cauaucugga caaacaaucu gauacgcagu acguguguaa gagaacucug      600

gucgacaggg gcugggggaa cggaugcggu cucuuuggca agggguccuu aguuaccugu      660

gcuaaauucg ccugcucaaa gaaaaugaca ggaaagagca uucagccuga gaaucuggaa      720

uaucggauca ugcuuagugu gcauggcucu cagcacuccg guaugauagu aaacgauacu      780

gggcaugaga ccgacgagaa ucgcgcgaaa guggaaauua caccaaacag cccccgagcc      840

gaggcaaccu ugggaggcuu uggcucgcug gggcuagauu gugaaccuag aacgggacuc      900

gacuucucug aucuguacua ccuuacuaug aauaacaagc acuggcuggu ccacaaggag      960

ugguuucaug acaucccauu gcccuggcac gcuggugccg acacaggcac cccucauugg     1020

aauaacaaag aagcucucgu ggaguucaag gaugcccacg caaaaaggca aacuguugug     1080

guccuggggu cccaggaggg agccgugcac acagcuuuag cgggcgcacu ggaagccgag     1140

auggacggug cuaaggggcg gcucucaagu ggccaucuua aaugccgucu gaagauggau     1200

aaauugcgcc uaaagggagu aagcuauucc cuguguaccg ccgcauuuac guucaccaag     1260

auuccagccg aaacacucca cggcacuguu accguggagg uccaguacgc ugggacagac     1320

ggaccgugca aagugcccgc ccagauggca gucgauaugc aaacucugac cccugugggu     1380

agacuuauca cagcgaaucc aguuaucacc gaaucuacug agaacagcaa gaugaugcug     1440

gaguuggauc ccccuuuugg cgacucauau auugugauag ggguaggaga gaaaaagauc     1500

acgcaucacu ggcauaggag uggcuccaca auugguaagg cuuucgaagc caccguccgg     1560

ggcgcaaaac gaauggccgu gcucggcgac acugcuuggg auuuuggaag cguuggcggg     1620

gccuuaaauu cucugggaaa ggguauccac cagauauucg gcgcagcuuu caaaucacug     1680

uuugggggaa ugaguugguu cagccagauu cuaaucggca cccuccugau guggcuuggu     1740

uugaacacaa agaacggguc caucucucug augugccucg cccugggcgg agugcuuauu     1800

uuucugucga ccgcggucag cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10161
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10161
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10162
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10162
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaccugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acgccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10163
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10163
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc aucaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaagcgacgc cggcaaggcc aucagcuucg ccaccacccu gggcgugaac      180

aagugccacg ugcagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucaaggugg agaacuggau cuucagaaac      480

cccggcuucg cccugguggc cguggccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucaccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucggcuac gagaccgacg aggacagagc caagguggag     1080

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaagaucc ccgugcagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1800

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1860

ugggacuucg gcagcguggg cggcguguuc aacagccugg gcaagggcau ccaccagauc     1920

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1980

ggcacccugc ugguguggcu gggccugaac accaagaacg gcagcaucag ccugaccugc     2040

cuggcccugg gcggcgugau gaucuuccug agcaccgccg ugagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10164
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10164
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1860

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1920

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1980

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     2040

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10165
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10165
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10166
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10166
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa cgccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10167
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10167
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagca ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1800

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1860

ggcguguuca acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1920

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gguguggcug     1980

ggccugaaca ccaagaacgg cagcaucagc cugaccugcc uggcccuggg cggcgugaug     2040

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10168
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10168
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcaccaucgg caaggccuuc     1800

gaggccaccg ugagaggcgc caagagaaug gccgugcugg gcgacaccgc cugggacuuc     1860

ggcagcgugg gcggcgcccu gaacagccug ggcaagggca uccaccagau cuucggcgcc     1920

gccuucaaga gccuguucgg cggcaugagc ugguucagcc agauccugau cggcacccug     1980

cugauguggc ugggccugaa caccaagaac ggcagcauca gccugaugug ccuggcccug     2040

ggcggcgugc ugaucuuccu gagcaccgcc gugagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10169
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10169
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10170
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10170
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac accugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga cguggugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaacg ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10171
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10171
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccauc accagaagag gcagcgccua cuacauguac      120

cuggacagaa gcgacgccgg caaggccauc agcuucgcca ccacccuggg cgugaacaag      180

ugccacgugc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc aagguggaga acuggaucuu cagaaacccc      480

ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga cguggugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucaccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cggcuacgag accgacgagg acagagccaa gguggaggug     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacuguucag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa ggugcccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aagauccccg ugcagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg acaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag     1800

gccuucgagg ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg     1860

gacuucggca gcgugggcgg cguguucaac agccugggca agggcaucca ccagaucuuc     1920

ggcgccgccu ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc     1980

acccugcugg uguggcuggg ccugaacacc aagaacggca gcaucagccu gaccugccug     2040

gcccugggcg gcgugaugau cuuccugagc accgccguga gcgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10172
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10172
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga cguggugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

accaucggca aggccuucga ggccaccgug agaggcgcca agagaauggc cgugcugggc     1860

gacaccgccu gggacuucgg cagcgugggc ggcgcccuga acagccuggg caagggcauc     1920

caccagaucu ucggcgccgc cuucaagagc cuguucggcg gcaugagcug guucagccag     1980

auccugaucg gcacccugcu gauguggcug ggccugaaca ccaagaacgg cagcaucagc     2040

cugaugugcc uggcccuggg cggcgugcug aucuuccuga gcaccgccgu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10173
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10173
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcacccugg gcaaggccuu cagcaccacc cugaagggcg cccagagacu ggccgcccug     1860

ggcgacaccg ccugggacuu cggcagcauc ggcggcgugu ucaacagcau cggcagagcc     1920

gugcaccagg uguucggcgg cgccuucaga acccuguucg gcggcaugag cuggaucacc     1980

cagggccuga ugggcgcccu gcugcugugg augggcguga acgccagaga cagaagcauc     2040

gcccuggccu uccuggccac cggcggcgug cugguguucc uggccaccaa cgugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10174
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10174
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaccugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcacccugg gcaaggccuu cagcaccacc cugaagggcg cccagagacu ggccgcccug     1860

ggcgacaccg ccugggacuu cggcagcauc ggcggcgugu ucaacagcau cggcagagcc     1920

gugcaccagg uguucggcgg cgccuucaga acccuguucg gcggcaugag cuggaucacc     1980

cagggccuga ugggcgcccu gcugcugugg augggcguga acgccagaga cagaagcauc     2040

gcccuggccu uccuggccac cggcggcgug cugguguucc uggccaccaa cgugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10175
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10175
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc aucaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaagcgacgc cggcaaggcc aucagcuucg ccaccacccu gggcgugaac      180

aagugccacg ugcagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucaaggugg agaacuggau cuucagaaac      480

cccggcuucg cccugguggc cguggccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucaccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucggcuac gagaccgacg aggacagagc caagguggag     1080

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug     1140

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac     1200

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc     1260

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1320

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1380

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac     1440

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1500

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1560

gaggugcagu acgccggcac cgacggcccc ugcaagaucc ccgugcagau ggccguggac     1620

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1680

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1740

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag cacccugggc     1800

aaggccuuca gcaccacccu gaagggcgcc cagagacugg ccgcccuggg cgacaccgcc     1860

ugggacuucg gcagcaucgg cggcguguuc aacagcaucg gcagagccgu gcaccaggug     1920

uucggcggcg ccuucagaac ccuguucggc ggcaugagcu ggaucaccca gggccugaug     1980

ggcgcccugc ugcuguggau gggcgugaac gccagagaca gaagcaucgc ccuggccuuc     2040

cuggccaccg gcggcgugcu gguguuccug gccaccaacg ugcacgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10176
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10176
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gugaccagaa gaggcagcgc cuacuacaug      120

uaccuggaca gaaacgacgc cggcgaggcc aucagcuucc ccaccacccu gggcaugaac      180

aagugcuaca uccagaucau ggaccugggc cacaugugcg acgccaccau gagcuacgag      240

ugccccaugc uggacgaggg cguggagccc gacgacgugg acugcuggug caacaccacc      300

agcaccuggg ugguguacgg caccugccac cacaagaagg gcgaggccag aagaagcaga      360

agagccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      420

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      480

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      540

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      600

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      660

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      720

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      780

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      840

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      900

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      960

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac     1020

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga     1080

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc     1140

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug     1200

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc     1260

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1320

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1380

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1440

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1500

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1560

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1620

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1680

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1740

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1800

agcacccugg gcaaggccuu cagcaccacc cugaagggcg cccagagacu ggccgcccug     1860

ggcgacaccg ccugggacuu cggcagcauc ggcggcgugu ucaacagcau cggcagagcc     1920

gugcaccagg uguucggcgg cgccuucaga acccuguucg gcggcaugag cuggaucacc     1980

cagggccuga ugggcgcccu gcugcugugg augggcguga acgccagaga cagaagcauc     2040

gcccuggccu uccuggccac cggcggcgug cugguguucc uggccaccaa cgugcacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10177
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10177
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacaucgugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10178
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10178
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaccugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10179
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10179
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagca ucaccagaag aggcagcgcc uacuacaugu accuggacag aagcgacgcc      120

ggcaaggcca ucagcuucgc caccacccug ggcgugaaca agugccacgu gcagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucaaggugga gaacuggauc uucagaaacc ccggcuucgc ccugguggcc      480

guggccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucaccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucggcuacg agaccgacga ggacagagcc aagguggagg ugacccccaa cagccccaga     1080

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc     1140

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag     1200

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1260

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1320

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1380

gagauggacg gcgccaaggg cagacuguuc agcggccacc ugaagugcag acugaagaug     1440

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1500

aaggugcccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1560

gacggccccu gcaagauccc cgugcagaug gccguggaca ugcagacccu gacccccgug     1620

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1680

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgacaagaag     1740

aucacccacc acuggcacag aagcggcagc acccugggca aggccuucag caccacccug     1800

aagggcgccc agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc     1860

ggcguguuca acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc     1920

cuguucggcg gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug     1980

ggcgugaacg ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug     2040

guguuccugg ccaccaacgu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10180
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10180
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ugaccagaag aggcagcgcc uacuacaugu accuggacag aaacgacgcc      120

ggcgaggcca ucagcuuccc caccacccug ggcaugaaca agugcuacau ccagaucaug      180

gaccugggcc acaugugcga cgccaccaug agcuacgagu gccccaugcu ggacgagggc      240

guggagcccg acgacgugga cugcuggugc aacaccacca gcaccugggu gguguacggc      300

accugccacc acaagaaggg cgaggccaga agaagcagaa gagccgugac ccugcccagc      360

cacagcacca gaaagcugca gaccagaagc cagaccuggc uggagagcag agaguacacc      420

aagcaccuga ucagagugga gaacuggauc uucagaaacc ccggcuucgc ccuggccgcc      480

gccgccaucg ccuggcugcu gggcagcagc accagccaga aggugaucua ccuggugaug      540

auccugcuga ucgcccccgc cuacagcauc agaugcaucg gcgugagcaa cagagacuuc      600

guggagggca ugagcggcgg caccugggug gacguggugc uggagcacgg cggcugcgug      660

accgugaugg cccaggacaa gcccaccgug gacaucgagc uggugaccac caccgugagc      720

aacauggccg aggugagaag cuacugcuac gaggccagca ucagcgacau ggccagcgac      780

agcagaugcc ccacccaggg cgaggccuac cuggacaagc agagcgacac ccaguacgug      840

ugcaagagaa cccuggugga cagaggcugg ggcaacggcu gcggccuguu cggcaagggc      900

agccugguga ccugcgccaa guucgccugc agcaagaaga ugaccggcaa gagcauccag      960

cccgagaacc uggaguacag aaucaugcug agcgugcacg gcagccagca cagcggcaug     1020

aucgugaacg acaccggcca cgagaccgac gagaacagag ccaaggugga gaucaccccc     1080

aacagcccca gagccgaggc cacccugggc ggcuucggca gccugggccu ggacugcgag     1140

cccagaaccg gccuggacuu cagcgaccug uacuaccuga ccaugaacaa caagcacugg     1200

cuggugcaca aggagugguu ccacgacauc ccccugcccu ggcacgccgg cgccgacacc     1260

ggcacccccc acuggaacaa caaggaggcc cugguggagu ucaaggacgc ccacgccaag     1320

agacagaccg ugguggugcu gggcagccag gagggcgccg ugcacaccgc ccuggccggc     1380

gcccuggagg ccgagaugga cggcgccaag ggcagacuga gcagcggcca ccugaagugc     1440

agacugaaga uggacaagcu gagacugaag ggcgugagcu acagccugug caccgccgcc     1500

uucaccuuca ccaagauccc cgccgagacc cugcacggca ccgugaccgu ggaggugcag     1560

uacgccggca ccgacggccc cugcaaggug cccgcccaga uggccgugga caugcagacc     1620

cugacccccg ugggcagacu gaucaccgcc aaccccguga ucaccgagag caccgagaac     1680

agcaagauga ugcuggagcu ggaccccccc uucggcgaca gcuacaucgu gaucggcgug     1740

ggcgagaaga agaucaccca ccacuggcac agaagcggca gcacccuggg caaggccuuc     1800

agcaccaccc ugaagggcgc ccagagacug gccgcccugg gcgacaccgc cugggacuuc     1860

ggcagcaucg gcggcguguu caacagcauc ggcagagccg ugcaccaggu guucggcggc     1920

gccuucagaa cccuguucgg cggcaugagc uggaucaccc agggccugau gggcgcccug     1980

cugcugugga ugggcgugaa cgccagagac agaagcaucg cccuggccuu ccuggccacc     2040

ggcggcgugc ugguguuccu ggccaccaac gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10181
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10181
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga caucgugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

acccugggca aggccuucag caccacccug aagggcgccc agagacuggc cgcccugggc     1860

gacaccgccu gggacuucgg cagcaucggc ggcguguuca acagcaucgg cagagccgug     1920

caccaggugu ucggcggcgc cuucagaacc cuguucggcg gcaugagcug gaucacccag     1980

ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg ccagagacag aagcaucgcc     2040

cuggccuucc uggccaccgg cggcgugcug guguuccugg ccaccaacgu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10182
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10182
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac accugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga cguggugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

acccugggca aggccuucag caccacccug aagggcgccc agagacuggc cgcccugggc     1860

gacaccgccu gggacuucgg cagcaucggc ggcguguuca acagcaucgg cagagccgug     1920

caccaggugu ucggcggcgc cuucagaacc cuguucggcg gcaugagcug gaucacccag     1980

ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg ccagagacag aagcaucgcc     2040

cuggccuucc uggccaccgg cggcgugcug guguuccugg ccaccaacgu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10183
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10183
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccauc accagaagag gcagcgccua cuacauguac      120

cuggacagaa gcgacgccgg caaggccauc agcuucgcca ccacccuggg cgugaacaag      180

ugccacgugc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc aagguggaga acuggaucuu cagaaacccc      480

ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga cguggugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucaccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cggcuacgag accgacgagg acagagccaa gguggaggug     1080

acccccaaca gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac     1140

ugcgagccca gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag     1200

cacuggcugg ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc     1260

gacaccggca ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac     1320

gccaagagac agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug     1380

gccggcgccc uggaggccga gauggacggc gccaagggca gacuguucag cggccaccug     1440

aagugcagac ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc     1500

gccgccuuca ccuucaccaa ggugcccgcc gagacccugc acggcaccgu gaccguggag     1560

gugcaguacg ccggcaccga cggccccugc aagauccccg ugcagauggc cguggacaug     1620

cagacccuga cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc     1680

gagaacagca agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc     1740

ggcgugggcg acaagaagau cacccaccac uggcacagaa gcggcagcac ccugggcaag     1800

gccuucagca ccacccugaa gggcgcccag agacuggccg cccugggcga caccgccugg     1860

gacuucggca gcaucggcgg cguguucaac agcaucggca gagccgugca ccagguguuc     1920

ggcggcgccu ucagaacccu guucggcggc augagcugga ucacccaggg ccugaugggc     1980

gcccugcugc uguggauggg cgugaacgcc agagacagaa gcaucgcccu ggccuuccug     2040

gccaccggcg gcgugcuggu guuccuggcc accaacgugc acgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10184
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10184
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgug accagaagag gcagcgccua cuacauguac      120

cuggacagaa acgacgccgg cgaggccauc agcuucccca ccacccuggg caugaacaag      180

ugcuacaucc agaucaugga ccugggccac augugcgacg ccaccaugag cuacgagugc      240

cccaugcugg acgagggcgu ggagcccgac gacguggacu gcuggugcaa caccaccagc      300

accugggugg uguacggcac cugccaccac aagaagggcg aggccagaag aagcagaaga      360

gccgugaccc ugcccagcca cagcaccaga aagcugcaga ccagaagcca gaccuggcug      420

gagagcagag aguacaccaa gcaccugauc agaguggaga acuggaucuu cagaaacccc      480

ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg gcagcagcac cagccagaag      540

gugaucuacc uggugaugau ccugcugauc gcccccgccu acagcaucag augcaucggc      600

gugagcaaca gagacuucgu ggagggcaug agcggcggca ccugggugga cguggugcug      660

gagcacggcg gcugcgugac cgugauggcc caggacaagc ccaccgugga caucgagcug      720

gugaccacca ccgugagcaa cauggccgag gugagaagcu acugcuacga ggccagcauc      780

agcgacaugg ccagcgacag cagaugcccc acccagggcg aggccuaccu ggacaagcag      840

agcgacaccc aguacgugug caagagaacc cugguggaca gaggcugggg caacggcugc      900

ggccuguucg gcaagggcag ccuggugacc ugcgccaagu ucgccugcag caagaagaug      960

accggcaaga gcauccagcc cgagaaccug gaguacagaa ucaugcugag cgugcacggc     1020

agccagcaca gcggcaugau cgugaacgac accggccacg agaccgacga gaacagagcc     1080

aagguggaga ucacccccaa cagccccaga gccgaggcca cccugggcgg cuucggcagc     1140

cugggccugg acugcgagcc cagaaccggc cuggacuuca gcgaccugua cuaccugacc     1200

augaacaaca agcacuggcu ggugcacaag gagugguucc acgacauccc ccugcccugg     1260

cacgccggcg ccgacaccgg caccccccac uggaacaaca aggaggcccu gguggaguuc     1320

aaggacgccc acgccaagag acagaccgug guggugcugg gcagccagga gggcgccgug     1380

cacaccgccc uggccggcgc ccuggaggcc gagauggacg gcgccaaggg cagacugagc     1440

agcggccacc ugaagugcag acugaagaug gacaagcuga gacugaaggg cgugagcuac     1500

agccugugca ccgccgccuu caccuucacc aagauccccg ccgagacccu gcacggcacc     1560

gugaccgugg aggugcagua cgccggcacc gacggccccu gcaaggugcc cgcccagaug     1620

gccguggaca ugcagacccu gacccccgug ggcagacuga ucaccgccaa ccccgugauc     1680

accgagagca ccgagaacag caagaugaug cuggagcugg acccccccuu cggcgacagc     1740

uacaucguga ucggcguggg cgagaagaag aucacccacc acuggcacag aagcggcagc     1800

acccugggca aggccuucag caccacccug aagggcgccc agagacuggc cgcccugggc     1860

gacaccgccu gggacuucgg cagcaucggc ggcguguuca acagcaucgg cagagccgug     1920

caccaggugu ucggcggcgc cuucagaacc cuguucggcg gcaugagcug gaucacccag     1980

ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg ccagagacag aagcaucgcc     2040

cuggccuucc uggccaccgg cggcgugcug guguuccugg ccaccaacgu gcacgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10185
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10185
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      120

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      180

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      240

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      300

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacaucgug      360

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      420

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      480

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      540

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      600

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      660

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac      720

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga      780

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc      840

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug      900

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc      960

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1020

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1080

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1140

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1200

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1260

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1320

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1380

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1440

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1500

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1560

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1620

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1680

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     1740

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10186
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10186
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      120

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      180

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      240

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      300

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      360

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      420

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      480

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      540

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      600

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      660

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac      720

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga      780

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc      840

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug      900

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc      960

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1020

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1080

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1140

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1200

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1260

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1320

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1380

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1440

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1500

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1560

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1620

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1680

cagauccuga ucggcacccu gcugaugugg cugggccuga acgccaagaa cggcagcauc     1740

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10187
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10187
gggagaaagc uuaccaugac cagcaucggc aucaucggcc ugcugcugac caccgccaug       60

gccgccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      120

cuggagagca gagaguacac caagcaccug aucaaggugg agaacuggau cuucagaaac      180

cccggcuucg cccugguggc cguggccauc gccuggcugc ugggcagcag caccagccag      240

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      300

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      360

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      420

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      480

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      540

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      600

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucaccug cagcaagaag      660

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac      720

ggcagccagc acagcggcau gaucggcuac gagaccgacg aggacagagc caagguggag      780

gugaccccca acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug      840

gacugcgagc ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac      900

aagcacuggc uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc      960

gccgacaccg gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc     1020

cacgccaaga gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc     1080

cuggccggcg cccuggaggc cgagauggac ggcgccaagg gcagacuguu cagcggccac     1140

cugaagugca gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc     1200

accgccgccu ucaccuucac caaggugccc gccgagaccc ugcacggcac cgugaccgug     1260

gaggugcagu acgccggcac cgacggcccc ugcaagaucc ccgugcagau ggccguggac     1320

augcagaccc ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc     1380

accgagaaca gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug     1440

aucggcgugg gcgacaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc     1500

aaggccuucg aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc     1560

ugggacuucg gcagcguggg cggcguguuc aacagccugg gcaagggcau ccaccagauc     1620

uucggcgccg ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc     1680

ggcacccugc ugguguggcu gggccugaac accaagaacg gcagcaucag ccugaccugc     1740

cuggcccugg gcggcgugau gaucuuccug agcaccgccg ugagcgccug aggacuaguu     1800

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     1860

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  10188
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10188
gggagaaagc uuaccaugac cagcgugggc aucgugggcc ugcugcugac caccgccaug       60

gccgccguga cccugcccag ccacagcacc agaaagcugc agaccagaag ccagaccugg      120

cuggagagca gagaguacac caagcaccug aucagagugg agaacuggau cuucagaaac      180

cccggcuucg cccuggccgc cgccgccauc gccuggcugc ugggcagcag caccagccag      240

aaggugaucu accuggugau gauccugcug aucgcccccg ccuacagcau cagaugcauc      300

ggcgugagca acagagacuu cguggagggc augagcggcg gcaccugggu ggacguggug      360

cuggagcacg gcggcugcgu gaccgugaug gcccaggaca agcccaccgu ggacaucgag      420

cuggugacca ccaccgugag caacauggcc gaggugagaa gcuacugcua cgaggccagc      480

aucagcgaca uggccagcga cagcagaugc cccacccagg gcgaggccua ccuggacaag      540

cagagcgaca cccaguacgu gugcaagaga acccuggugg acagaggcug gggcaacggc      600

ugcggccugu ucggcaaggg cagccuggug accugcgcca aguucgccug cagcaagaag      660

augaccggca agagcaucca gcccgagaac cuggaguaca gaaucaugcu gagcgugcac      720

ggcagccagc acagcggcau gaucgugaac gacaccggcc acgagaccga cgagaacaga      780

gccaaggugg agaucacccc caacagcccc agagccgagg ccacccuggg cggcuucggc      840

agccugggcc uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug      900

accaugaaca acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc      960

uggcacgccg gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag     1020

uucaaggacg cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc     1080

gugcacaccg cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug     1140

agcagcggcc accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc     1200

uacagccugu gcaccgccgc cuucaccuuc accaagaucc ccgccgagac ccugcacggc     1260

accgugaccg uggaggugca guacgccggc accgacggcc ccugcaaggu gcccgcccag     1320

auggccgugg acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug     1380

aucaccgaga gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac     1440

agcuacaucg ugaucggcgu gggcgagaag aagaucaccc accacuggca cagaagcggc     1500

agcaccaucg gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug     1560

ggcgacaccg ccugggacuu cggcagcgug ggcggcgccc ugaacagccu gggcaagggc     1620

auccaccaga ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc     1680

cagauccuga ucggcacccu gcugaugugg cugggccuga acaccaagaa cggcagcauc     1740

agccugaugu gccuggcccu gggcggcgug cugaucuucc ugagcaccgc cgugagcgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10189
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10189
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gccgugaccc ugcccagcca cagcaccaga      120

aagcugcaga ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc      180

agaguggaga acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc      240

uggcugcugg gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc      300

gcccccgccu acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug      360

agcggcggca ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc      420

caggacaagc ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag      480

gugagaagcu acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc      540

acccagggcg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc      600

cugguggaca gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc      660

ugcgccaagu ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug      720

gaguacagaa ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac      780

accggccacg agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga      840

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc      900

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag      960

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1020

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1080

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1140

gagauggacg gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug     1200

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1260

aagauccccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1320

gacggccccu gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug     1380

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1440

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag     1500

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1560

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1620

ggcgcccuga acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1680

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug     1740

ggccugaaca ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug     1800

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10190
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10190
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gccgugaccc ugcccagcca cagcaccaga      120

aagcugcaga ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc      180

agaguggaga acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc      240

uggcugcugg gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc      300

gcccccgccu acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug      360

agcggcggca ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc      420

caggacaagc ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag      480

gugagaagcu acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc      540

acccagggcg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc      600

cugguggaca gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc      660

ugcgccaagu ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug      720

gaguacagaa ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac      780

accggccacg agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga      840

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc      900

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag      960

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1020

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1080

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1140

gagauggacg gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug     1200

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1260

aagauccccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1320

gacggccccu gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug     1380

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1440

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag     1500

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1560

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1620

ggcgcccuga acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1680

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug     1740

ggccugaacg ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug     1800

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10191
<211>  2020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10191
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gccgugaccc ugcccagcca cagcaccaga      120

aagcugcaga ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc      180

aagguggaga acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc      240

uggcugcugg gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc      300

gcccccgccu acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug      360

agcggcggca ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc      420

caggacaagc ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag      480

gugagaagcu acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc      540

acccagggcg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc      600

cugguggaca gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc      660

ugcgccaagu ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug      720

gaguacagaa ucaugcugag cgugcacggc agccagcaca gcggcaugau cggcuacgag      780

accgacgagg acagagccaa gguggaggug acccccaaca gccccagagc cgaggccacc      840

cugggcggcu ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc      900

gaccuguacu accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac      960

gacauccccc ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag     1020

gaggcccugg uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc     1080

agccaggagg gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc     1140

gccaagggca gacuguucag cggccaccug aagugcagac ugaagaugga caagcugaga     1200

cugaagggcg ugagcuacag ccugugcacc gccgccuuca ccuucaccaa ggugcccgcc     1260

gagacccugc acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc     1320

aagauccccg ugcagauggc cguggacaug cagacccuga cccccguggg cagacugauc     1380

accgccaacc ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac     1440

ccccccuucg gcgacagcua caucgugauc ggcgugggcg acaagaagau cacccaccac     1500

uggcacagaa gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag     1560

agaauggccg ugcugggcga caccgccugg gacuucggca gcgugggcgg cguguucaac     1620

agccugggca agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc     1680

augagcuggu ucagccagau ccugaucggc acccugcugg uguggcuggg ccugaacacc     1740

aagaacggca gcaucagccu gaccugccug gcccugggcg gcgugaugau cuuccugagc     1800

accgccguga gcgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     1860

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1980

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2020


<210>  10192
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10192
gggagaaagc uuaccauggc caucagcggc gugcccgugc ugggcuucuu caucaucgcc       60

gugcugauga gcgcccagga gagcugggcc gccgugaccc ugcccagcca cagcaccaga      120

aagcugcaga ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc      180

agaguggaga acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc      240

uggcugcugg gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc      300

gcccccgccu acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug      360

agcggcggca ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc      420

caggacaagc ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag      480

gugagaagcu acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc      540

acccagggcg aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc      600

cugguggaca gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc      660

ugcgccaagu ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug      720

gaguacagaa ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac      780

accggccacg agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga      840

gccgaggcca cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc      900

cuggacuuca gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag      960

gagugguucc acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac     1020

uggaacaaca aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug     1080

guggugcugg gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc     1140

gagauggacg gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug     1200

gacaagcuga gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc     1260

aagauccccg ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc     1320

gacggccccu gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug     1380

ggcagacuga ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug     1440

cuggagcugg acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag     1500

aucacccacc acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug     1560

agaggcgcca agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc     1620

ggcgcccuga acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc     1680

cuguucggcg gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug     1740

ggccugaaca ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug     1800

aucuuccuga gcaccgccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10193
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10193
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      120

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      180

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      240

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      300

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      360

aucgugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      420

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      480

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      540

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      600

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      660

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      720

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      780

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      840

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      900

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      960

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1020

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1080

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1140

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1200

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1260

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1320

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1380

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1440

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1500

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1560

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1620

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1680

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     1740

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     1800

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10194
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10194
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      120

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      180

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      240

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      300

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      360

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      420

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      480

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      540

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      600

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      660

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      720

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      780

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      840

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      900

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      960

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1020

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1080

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1140

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1200

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1260

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1320

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1380

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1440

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1500

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1560

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1620

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1680

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacgc caagaacggc     1740

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     1800

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10195
<211>  1999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10195
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      120

accuggcugg agagcagaga guacaccaag caccugauca agguggagaa cuggaucuuc      180

agaaaccccg gcuucgcccu gguggccgug gccaucgccu ggcugcuggg cagcagcacc      240

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      300

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      360

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      420

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      480

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      540

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      600

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu caccugcagc      660

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      720

gugcacggca gccagcacag cggcaugauc ggcuacgaga ccgacgagga cagagccaag      780

guggagguga cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug      840

ggccuggacu gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug      900

aacaacaagc acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac      960

gccggcgccg acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag     1020

gacgcccacg ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac     1080

accgcccugg ccggcgcccu ggaggccgag auggacggcg ccaagggcag acuguucagc     1140

ggccaccuga agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc     1200

cugugcaccg ccgccuucac cuucaccaag gugcccgccg agacccugca cggcaccgug     1260

accguggagg ugcaguacgc cggcaccgac ggccccugca agauccccgu gcagauggcc     1320

guggacaugc agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc     1380

gagagcaccg agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac     1440

aucgugaucg gcgugggcga caagaagauc acccaccacu ggcacagaag cggcagcacc     1500

aucggcaagg ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac     1560

accgccuggg acuucggcag cgugggcggc guguucaaca gccugggcaa gggcauccac     1620

cagaucuucg gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc     1680

cugaucggca cccugcuggu guggcugggc cugaacacca agaacggcag caucagccug     1740

accugccugg cccugggcgg cgugaugauc uuccugagca ccgccgugag cgccugagga     1800

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     1860

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     1980

uuucagagcc accagaauu                                                  1999


<210>  10196
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10196
gggagaaagc uuaccaugga cuggaccugg auccuguucc ugguggccgc cgccaccaga       60

gugcacagcg ccgugacccu gcccagccac agcaccagaa agcugcagac cagaagccag      120

accuggcugg agagcagaga guacaccaag caccugauca gaguggagaa cuggaucuuc      180

agaaaccccg gcuucgcccu ggccgccgcc gccaucgccu ggcugcuggg cagcagcacc      240

agccagaagg ugaucuaccu ggugaugauc cugcugaucg cccccgccua cagcaucaga      300

ugcaucggcg ugagcaacag agacuucgug gagggcauga gcggcggcac cuggguggac      360

guggugcugg agcacggcgg cugcgugacc gugauggccc aggacaagcc caccguggac      420

aucgagcugg ugaccaccac cgugagcaac auggccgagg ugagaagcua cugcuacgag      480

gccagcauca gcgacauggc cagcgacagc agaugcccca cccagggcga ggccuaccug      540

gacaagcaga gcgacaccca guacgugugc aagagaaccc ugguggacag aggcuggggc      600

aacggcugcg gccuguucgg caagggcagc cuggugaccu gcgccaaguu cgccugcagc      660

aagaagauga ccggcaagag cauccagccc gagaaccugg aguacagaau caugcugagc      720

gugcacggca gccagcacag cggcaugauc gugaacgaca ccggccacga gaccgacgag      780

aacagagcca agguggagau cacccccaac agccccagag ccgaggccac ccugggcggc      840

uucggcagcc ugggccugga cugcgagccc agaaccggcc uggacuucag cgaccuguac      900

uaccugacca ugaacaacaa gcacuggcug gugcacaagg agugguucca cgacaucccc      960

cugcccuggc acgccggcgc cgacaccggc accccccacu ggaacaacaa ggaggcccug     1020

guggaguuca aggacgccca cgccaagaga cagaccgugg uggugcuggg cagccaggag     1080

ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg agauggacgg cgccaagggc     1140

agacugagca gcggccaccu gaagugcaga cugaagaugg acaagcugag acugaagggc     1200

gugagcuaca gccugugcac cgccgccuuc accuucacca agauccccgc cgagacccug     1260

cacggcaccg ugaccgugga ggugcaguac gccggcaccg acggccccug caaggugccc     1320

gcccagaugg ccguggacau gcagacccug acccccgugg gcagacugau caccgccaac     1380

cccgugauca ccgagagcac cgagaacagc aagaugaugc uggagcugga cccccccuuc     1440

ggcgacagcu acaucgugau cggcgugggc gagaagaaga ucacccacca cuggcacaga     1500

agcggcagca ccaucggcaa ggccuucgag gccaccguga gaggcgccaa gagaauggcc     1560

gugcugggcg acaccgccug ggacuucggc agcgugggcg gcgcccugaa cagccugggc     1620

aagggcaucc accagaucuu cggcgccgcc uucaagagcc uguucggcgg caugagcugg     1680

uucagccaga uccugaucgg cacccugcug auguggcugg gccugaacac caagaacggc     1740

agcaucagcc ugaugugccu ggcccugggc ggcgugcuga ucuuccugag caccgccgug     1800

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10197
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10197
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgcc gugacccugc ccagccacag caccagaaag      120

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      180

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      240

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      300

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      360

ggcggcaccu ggguggacau cgugcuggag cacggcggcu gcgugaccgu gauggcccag      420

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      480

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      540

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      600

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      660

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      720

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      780

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      840

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      900

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      960

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1020

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1080

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1140

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1200

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1260

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1320

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1380

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1440

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1500

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1560

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1620

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1680

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1740

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     1800

uuccugagca ccgccgugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10198
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10198
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgcc gugacccugc ccagccacag caccagaaag      120

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      180

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      240

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      300

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      360

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      420

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      480

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      540

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      600

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      660

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      720

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      780

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      840

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      900

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      960

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1020

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1080

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1140

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1200

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1260

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1320

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1380

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1440

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1500

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1560

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1620

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1680

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1740

cugaacgcca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     1800

uuccugagca ccgccgugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10199
<211>  2017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10199
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgcc gugacccugc ccagccacag caccagaaag      120

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaag      180

guggagaacu ggaucuucag aaaccccggc uucgcccugg uggccguggc caucgccugg      240

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      300

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      360

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      420

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      480

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      540

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      600

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      660

gccaaguuca ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      720

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgg cuacgagacc      780

gacgaggaca gagccaaggu ggaggugacc cccaacagcc ccagagccga ggccacccug      840

ggcggcuucg gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac      900

cuguacuacc ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac      960

aucccccugc ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag     1020

gcccuggugg aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc     1080

caggagggcg ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc     1140

aagggcagac uguucagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug     1200

aagggcguga gcuacagccu gugcaccgcc gccuucaccu ucaccaaggu gcccgccgag     1260

acccugcacg gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag     1320

auccccgugc agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc     1380

gccaaccccg ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc     1440

cccuucggcg acagcuacau cgugaucggc gugggcgaca agaagaucac ccaccacugg     1500

cacagaagcg gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga     1560

auggccgugc ugggcgacac cgccugggac uucggcagcg ugggcggcgu guucaacagc     1620

cugggcaagg gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug     1680

agcugguuca gccagauccu gaucggcacc cugcuggugu ggcugggccu gaacaccaag     1740

aacggcagca ucagccugac cugccuggcc cugggcggcg ugaugaucuu ccugagcacc     1800

gccgugagcg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     1860

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1980

ccccccccca aaggcucuuu ucagagccac cagaauu                              2017


<210>  10200
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10200
gggagaaagc uuaccauggg caagagaagc gccggcagca ucauguggcu ggccagccug       60

gccgugguga ucgccugcgc cggcgccgcc gugacccugc ccagccacag caccagaaag      120

cugcagacca gaagccagac cuggcuggag agcagagagu acaccaagca ccugaucaga      180

guggagaacu ggaucuucag aaaccccggc uucgcccugg ccgccgccgc caucgccugg      240

cugcugggca gcagcaccag ccagaaggug aucuaccugg ugaugauccu gcugaucgcc      300

cccgccuaca gcaucagaug caucggcgug agcaacagag acuucgugga gggcaugagc      360

ggcggcaccu ggguggacgu ggugcuggag cacggcggcu gcgugaccgu gauggcccag      420

gacaagccca ccguggacau cgagcuggug accaccaccg ugagcaacau ggccgaggug      480

agaagcuacu gcuacgaggc cagcaucagc gacauggcca gcgacagcag augccccacc      540

cagggcgagg ccuaccugga caagcagagc gacacccagu acgugugcaa gagaacccug      600

guggacagag gcuggggcaa cggcugcggc cuguucggca agggcagccu ggugaccugc      660

gccaaguucg ccugcagcaa gaagaugacc ggcaagagca uccagcccga gaaccuggag      720

uacagaauca ugcugagcgu gcacggcagc cagcacagcg gcaugaucgu gaacgacacc      780

ggccacgaga ccgacgagaa cagagccaag guggagauca cccccaacag ccccagagcc      840

gaggccaccc ugggcggcuu cggcagccug ggccuggacu gcgagcccag aaccggccug      900

gacuucagcg accuguacua ccugaccaug aacaacaagc acuggcuggu gcacaaggag      960

ugguuccacg acaucccccu gcccuggcac gccggcgccg acaccggcac cccccacugg     1020

aacaacaagg aggcccuggu ggaguucaag gacgcccacg ccaagagaca gaccguggug     1080

gugcugggca gccaggaggg cgccgugcac accgcccugg ccggcgcccu ggaggccgag     1140

auggacggcg ccaagggcag acugagcagc ggccaccuga agugcagacu gaagauggac     1200

aagcugagac ugaagggcgu gagcuacagc cugugcaccg ccgccuucac cuucaccaag     1260

auccccgccg agacccugca cggcaccgug accguggagg ugcaguacgc cggcaccgac     1320

ggccccugca aggugcccgc ccagauggcc guggacaugc agacccugac ccccgugggc     1380

agacugauca ccgccaaccc cgugaucacc gagagcaccg agaacagcaa gaugaugcug     1440

gagcuggacc cccccuucgg cgacagcuac aucgugaucg gcgugggcga gaagaagauc     1500

acccaccacu ggcacagaag cggcagcacc aucggcaagg ccuucgaggc caccgugaga     1560

ggcgccaaga gaauggccgu gcugggcgac accgccuggg acuucggcag cgugggcggc     1620

gcccugaaca gccugggcaa gggcauccac cagaucuucg gcgccgccuu caagagccug     1680

uucggcggca ugagcugguu cagccagauc cugaucggca cccugcugau guggcugggc     1740

cugaacacca agaacggcag caucagccug augugccugg cccugggcgg cgugcugauc     1800

uuccugagca ccgccgugag cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10201
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10201
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gugaccagac ggggaagcgc uuacuauaug      120

uaccuggacc gcaacgaugc cggcgaagcc auuuccuucc cuacaacgcu ugggaugaau      180

aagugcuaua uccaaauuau ggaccuggga cacaugugug augcuaccau gucuuacgag      240

ugcccgaugc ucgacgaagg cguggagccc gaugacgucg auuguuggug caacacaacc      300

agcacauggg ugguuuaugg gaccugucau cacaaaaagg gagaagccag acggucccgc      360

agagcuguga cacugccuuc ucauagcacc cggaaacuuc agacgcgcuc ccagaccugg      420

cuggagagca gagaguacac aaagcaccuc auccgggucg aaaauuggau cuuucgcaac      480

cccggcuucg cccuggcggc cgcugccauu gcuuggcugc ucgggucuuc caccagccag      540

aaagugaucu aucugguuau gauucuucug aucgccccug cguacucuau uagaugcauc      600

ggggugucca aucgggacuu ugucgaggga augagcggcg ggacaugggu ggauauugug      660

cucgaacaug gaggcugugu caccguuaug gcccaagaca agcccacggu ggauaucgag      720

cuggugacaa ccacagucuc uaacauggcu gaggugcgcu ccuacugcua ugaagccagc      780

aucuccgaca uggcuucuga uagcagaugu ccuacccagg gggaggccua ccuugacaag      840

caguccgaca cccaguaugu uugcaaacgg acacuggugg aucgcggaug gggcaauggc      900

ugugggcugu ucggaaaggg cagccugguc acgugcgcca aauuugcuug cucuaagaaa      960

augaccggga aguccauuca accggaaaac cuggaguaca gaaucaugcu cagcgugcac     1020

ggaucucagc acagcggcau gauuguuaau gacacagggc augaaaccga ugagaaccgg     1080

gcgaaggugg agaucacacc caauuccccu agagccgaag cuacccuggg aggcuucggc     1140

ucucuugggc uggacuguga gccccgcacg ggacucgauu uuagcgaccu guauuaccuu     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgauau uccucucccc     1260

uggcacgccg gcgccgacac cgggaccccg cauuggaaca auaaagaggc ucugguggaa     1320

uuuaaggaug cccacgcgaa acggcagaca gucgugguuc ugggauccca ggagggcgcu     1380

gugcauaccg cccucgccgg ggcucuggag gccgaaaugg acggcgcuaa gggaagacuu     1440

agcucuggcc accugaaaug ccgccucaag auggauaagc ugcggcuuaa aggggucucc     1500

uauagccugu guacagccgc guucacguuu accaagaucc cugccgagac acuccaugga     1560

accgugacag uugaagugca auacgcuggc accgacgggc ccugcaaagu cccugcccag     1620

auggcugugg acaugcagac gcugaccccc gugggaagac ugaucacagc caaccccguu     1680

auuaccgagu cuacagaaaa cuccaagaug augcucgagc uggauccucc guucggcgac     1740

agcuacaucg ucauuggggu gggcgagaaa aagaucaccc accacuggca ucgcuccgga     1800

ucuacgauug gcaaggcguu cgaagccaca gugcggggcg cuaaaagaau ggccguccuu     1860

ggagauaccg cuugggacuu uggcagcguu gggggagccc ucaauucccu cggcaagggg     1920

auccaccaaa ucuucggcgc cgcuuuuaaa ucucuguucg gaggcaugag cugguuuucc     1980

cagauucuga ucgggacacu ucucaugugg cugggacuga acaccaagaa uggcagcauu     2040

ucucucaugu gucuggcgcu ugggggagug cugaucuucc ucuccaccgc cgugagcgcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10202
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10202
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gugaccagac ggggaagcgc uuacuauaug      120

uaccuggacc gcaacgaugc cggcgaagcc auuuccuucc cuacaacgcu ugggaugaau      180

aagugcuaua uccaaauuau ggaccuggga cacaccugug augcuacaau gucuuacgag      240

ugcccgaugc ucgacgaagg cguggagccc gaugacgucg auuguuggug caacaccaca      300

agcaccuggg ugguuuaugg gacaugucau cacaaaaagg gagaagccag acggucccgc      360

agagcuguga cccugccuuc ucauagcacg cggaaacuuc agacccgcuc ccagacaugg      420

cuggagagca gagaguacac caagcaccuc auccgggucg aaaauuggau cuuucgcaac      480

cccggcuucg cccuggcggc cgcugccauu gcuuggcugc ucgggucuuc cacaagccag      540

aaagugaucu aucugguuau gauucuucug aucgccccug cguacucuau uagaugcauc      600

ggggugucca aucgggacuu ugucgaggga augagcggcg ggaccugggu ggaugugguc      660

cucgaacaug gaggcugugu uacggugaug gcccaagaca agcccacagu ggauauugag      720

cuggucacca caaccguguc uaacauggcu gagguucgcu ccuacugcua ugaagccagc      780

aucuccgaca uggcuucuga uagcagaugu ccuacccagg gggaggccua ccuugacaag      840

caguccgaca cacaguaugu gugcaaacgg acgcuggucg aucgcggaug gggcaauggc      900

ugugggcugu ucggaaaggg cagccuggug accugcgcca aauuugcuug cucuaagaaa      960

augacaggga aguccaucca accggaaaac cuggaguaca gaauuaugcu cagcguucac     1020

ggaucucagc acagcggcau gaucgugaau gacaccgggc augaaacaga ugagaaccgg     1080

gcgaaggucg agauuacccc caauuccccu agagccgaag cuacgcuggg aggcuucggc     1140

ucucuugggc uggacuguga gccccgcaca ggacucgauu uuagcgaccu guauuaccuu     1200

accaugaaca auaaacacug gcuggugcau aaggaauggu uccacgauau cccucucccc     1260

uggcacgccg gcgccgacac cgggacaccg cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggaug cccacgcgaa acggcagacc gugguugugc ugggauccca ggagggcgcu     1380

guccauacag cccucgccgg ggcucuggag gccgaaaugg acggcgcuaa gggaagacuu     1440

agcucuggcc accugaaaug ccgccucaag auggauaagc ugcggcuuaa aggggugucc     1500

uauagccugu guacggccgc guucaccuuu acaaagauuc cugccgagac ccuccaugga     1560

acaguuaccg uggaagucca auacgcuggc acggacgggc ccugcaaagu gccugcccag     1620

auggcugugg acaugcagac ccugacaccc guuggaagac ugaucaccgc caaccccguc     1680

aucacagagu cuaccgaaaa cuccaagaug augcucgagc uggauccucc guucggcgac     1740

agcuacauug ugaucggggu gggcgagaaa aagauuacgc accacuggca ucgcuccgga     1800

ucuacaaucg gcaaggcguu cgaagccacc guccggggcg cuaaaagaau ggccgugcuu     1860

ggagauacag cuugggacuu uggcagcguu gggggagccc ucaauucccu cggcaagggg     1920

auucaccaaa ucuucggcgc cgcuuuuaaa ucucuguucg gaggcaugag cugguuuucc     1980

cagauccuga uugggacccu ucucaugugg cugggacuga acgcgaagaa uggcagcauc     2040

ucucucaugu gucuggcccu ugggggagug cugauuuucc ucuccaccgc ugucagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10203
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10203
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg auuaccagac ggggaagcgc uuacuauaug      120

uaccuggacc gcuccgaugc cggcaaggcc aucucuuucg cuacaacgcu uggggugaac      180

aaaugccacg ugcaaauuau ggaccuggga cauaugugug augccaccau gagcuaugaa      240

ugcccuaugc ucgacgaggg cgucgaaccg gaugacgugg auuguuggug caauacaacc      300

uccacauggg uuguguacgg gaccugucac cauaagaaag gagaggcuag acggucucgc      360

agagccguca cacugcccag ccacuccacc cggaagcugc agacgcgcag ccagaccugg      420

cuggaaucua gagaguauac aaaacaucuc aucaaggugg agaacuggau cuuucggaau      480

ccuggcuucg cgcugguugc cguggcuauu gccuggcugc ucggcuccag caccucucag      540

aaggucaucu accuggugau gauucuucug aucgcucccg ccuauuccau ucgcugcauc      600

ggggugagca acagagacuu ugucgaagga augucuggcg ggacaugggu ugauguggug      660

cucgagcacg gaggcugugu caccgugaug gcgcaagaca aaccuacggu ugauauugaa      720

cuggugacaa ccacagucuc caauauggcc gaggugcgga gcuacugcua cgaggcuucc      780

aucucugaca uggccagcga uucccgcugu cccacccagg gggaggcuua ucuugacaag      840

cagagcgaca cccaguacgu uugcaaaaga acacuggugg aucggggaug gggcaacggc      900

uguggacucu ucgggaaggg cucucugguc acgugcgcca aauuuaccug cuccaagaag      960

augacaggga aaagcaucca accugaaaau cuggaauauc gcauuaugcu cucugugcac     1020

ggaagccagc auuccggcau gaucggguac gagaccgacg aagauagagc caaggucgag     1080

gugacaccga acucuccccg ggcugaggcg acccugggag gcuucggcag ccuugggcug     1140

gacugugaac cuagaacggg acucgauuuu uccgaccugu auuaccuuac aaugaauaac     1200

aaacacuggc ugguucauaa ggagugguuc cacgauauuc cccucccuug gcacgccggc     1260

gcugacaccg ggacacccca uuggaauaac aaagaagccc ugguggaguu uaaggaugcc     1320

cacgcuaaac gccagaccgu cgugguucug ggaagccagg aaggcgccgu gcauaccgcg     1380

cucgcugggg cccuggaggc cgagauggac ggcgcuaagg gacggcuuuu cucuggccac     1440

cugaagugca gacucaaaau ggauaagcug cgccuuaaag gggucuccua uagccugugu     1500

acagccgcuu uuacguucac caaggugccg gccgaaacac uccauggaac cgugacaguu     1560

gagguccaau acgcgggcac cgacgggccu ugcaaaaucc ccgugcagau ggccguggac     1620

augcagacgc ugaccccugu cggacggcug auuacagcua aucccgugau caccgaaucu     1680

acagagaacu ccaagaugau gcucgaacug gaucccccuu ucggcgacag cuacaucguu     1740

auuggggugg gcgauaagaa aaucacccac cacuggcaua gauccggauc uacgauuggc     1800

aaggccuuug aggcuacagu ccgcggggcc aaacggaugg cggugcuugg agacaccgcc     1860

ugggauuucg gcagcguugg gggaguguuu aauucccugg gcaaggggau ccaccaaauu     1920

uucggcgcug ccuuuaaauc ucucuucgga ggcaugagcu gguuuuccca gauccugauc     1980

gggacacugc uugucuggcu cggacugaac accaagaacg gcagcauuuc ucugaccugu     2040

cucgcucuug ggggagugau gaucuuccuu uccacagccg uuagcgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10204
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10204
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gugaccagac ggggaagcgc uuacuauaug      120

uaccuggacc gcaacgaugc cggcgaagcc auuuccuucc cuacaacgcu ugggaugaau      180

aagugcuaua uccaaauuau ggaccuggga cacaugugug augcuaccau gucuuacgag      240

ugcccgaugc ucgacgaagg cguggagccc gaugacgucg auuguuggug caacacaacc      300

agcacauggg ugguuuaugg gaccugucau cacaaaaagg gagaagccag acggucccgc      360

agagcuguga cacugccuuc ucauagcacc cggaaacuuc agacgcgcuc ccagaccugg      420

cuggagagca gagaguacac aaagcaccuc auccgggucg aaaauuggau cuuucgcaac      480

cccggcuucg cccuggcggc cgcugccauu gcuuggcugc ucgggucuuc caccagccag      540

aaagugaucu aucugguuau gauucuucug aucgccccug cguacucuau uagaugcauc      600

ggggugucca aucgggacuu ugucgaggga augagcggcg ggacaugggu ggaugugguc      660

cucgaacaug gaggcugugu uaccgugaug gcccaagaca agcccacggu ggauauugag      720

cuggucacaa ccacaguguc uaacauggcu gagguucgcu ccuacugcua ugaagccagc      780

aucuccgaca uggcuucuga uagcagaugu ccuacccagg gggaggccua ccuugacaag      840

caguccgaca cccaguaugu gugcaaacgg acacuggucg aucgcggaug gggcaauggc      900

ugugggcugu ucggaaaggg cagccuggug acgugcgcca aauuugcuug cucuaagaaa      960

augaccggga aguccaucca accggaaaac cuggaguaca gaauuaugcu cagcguucac     1020

ggaucucagc acagcggcau gaucgugaau gacacagggc augaaaccga ugagaaccgg     1080

gcgaaggucg agauuacacc caauuccccu agagccgaag cuacccuggg aggcuucggc     1140

ucucuugggc uggacuguga gccccgcacg ggacucgauu uuagcgaccu guauuaccuu     1200

acaaugaaca auaaacacug gcuggugcau aaggaauggu uccacgauau cccucucccc     1260

uggcacgccg gcgccgacac cgggaccccg cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggaug cccacgcgaa acggcagaca gugguugugc ugggauccca ggagggcgcu     1380

guccauaccg cccucgccgg ggcucuggag gccgaaaugg acggcgcuaa gggaagacuu     1440

agcucuggcc accugaaaug ccgccucaag auggauaagc ugcggcuuaa aggggugucc     1500

uauagccugu guacagccgc guucacguuu accaagauuc cugccgagac acuccaugga     1560

accguuacag uggaagucca auacgcuggc accgacgggc ccugcaaagu gccugcccag     1620

auggcugugg acaugcagac gcugaccccc guuggaagac ugaucacagc caaccccguc     1680

aucaccgagu cuacagaaaa cuccaagaug augcucgagc uggauccucc guucggcgac     1740

agcuacauug ugaucggggu gggcgagaaa aagauuaccc accacuggca ucgcuccgga     1800

ucuacgaucg gcaaggcguu cgaagccaca guccggggcg cuaaaagaau ggccgugcuu     1860

ggagauaccg cuugggacuu uggcagcguu gggggagccc ucaauucccu cggcaagggg     1920

auucaccaaa ucuucggcgc cgcuuuuaaa ucucuguucg gaggcaugag cugguuuucc     1980

cagauccuga uugggacacu ucucaugugg cugggacuga acaccaagaa uggcagcauc     2040

ucucucaugu gucuggcgcu ugggggagug cugauuuucc ucuccaccgc cgucagcgcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10205
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10205
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg uuacgcggcg cggcuccgcg uacuauaugu accuggauag aaacgacgcu      120

ggggaggcca uuucuuuucc caccacacuu ggaaugaaua agugcuauau ccagauuaug      180

gaucugggcc auauguguga cgccaccaug agcuacgaau gcccuaugcu cgaugagggg      240

guggaaccgg acgaugugga cuguuggugc aacacaaccu ccacaugggu cguguaugga      300

accugucacc auaaaaaggg cgaggcucgg cgcucuagac gggccguuac gcugcccagc      360

cacuccaccc gcaaacuuca aacaagaucu cagaccuggc uggaaagccg ggaguacaca      420

aagcaucuca uccgcgugga gaauuggauu uucagaaacc cuggguuugc ucuggccgcg      480

gccgcuaucg ccuggcugcu cggauccagc accucucaga aagucaucua ucuggugaug      540

auucuucuga ucgcucccgc cuacuccauu cggugcaucg gcguuagcaa ucgcgauuuc      600

guggaaggca ugucuggggg aacguggguc gacauugugc ucgagcacgg cgggugugug      660

acagucaugg cgcaggauaa gccuaccguu gacaucgaac uggugacaac caccgugucc      720

aacauggccg aggucagaag cuacugcuau gaggcuucua uuuccgauau ggccagcgac      780

ucccgguguc ccacacaagg agaggcuuac cuugacaagc agucugauac gcaguaugug      840

ugcaaacgca cccugguuga cagaggcugg gggaauggau guggccucuu uggcaagggg      900

agccugguga caugcgccaa auucgccugc uccaagaaaa ugaccggaaa gagcauccag      960

ccugaaaacc uggaauaccg gaucaugcuc ucuguccacg gcucccaaca uagcgggaug     1020

auugugaaug auacaggaca cgagaccgac gaaaacagag cuaagguuga gaucacgccc     1080

aauucucccc gcgcggaggc cacacugggc ggguuuggaa gccuuggccu ggauugugaa     1140

ccucggaccg gccucgacuu cuccgaucug uauuaccuua ccaugaacaa uaaacauugg     1200

cuggugcaca aggagugguu ucacgacauu ccccucccuu ggcaugcugg ggccgauaca     1260

ggaaccccgc acuggaacaa uaaagaagcc cuggucgagu ucaaggacgc ucaugccaaa     1320

agacagacag uggucgugcu gggcucucag gaaggggcgg uucacacggc ucucgccgga     1380

gcccuggagg cugagaugga uggcgccaag gggcgccuua gcuccggcca ucugaaaugc     1440

cggcucaaga uggacaagcu gagacuuaaa ggagugagcu auucucugug uaccgcugcc     1500

uuuacauuca ccaagauccc ggcggaaaca cuccacggca ccgucacggu ggagguucag     1560

uacgccggga ccgacggacc uugcaaagug cccgcucaaa uggccgucga uaugcagaca     1620

cugaccccug ugggccgccu gauuacagcu aaccccguga ucaccgaauc cacggagaac     1680

agcaagauga ugcucgaacu ggaucccccu uuuggggacu cuuacaucgu uauuggaguc     1740

ggcgagaaaa agaucacaca ccauuggcac cgguccggga gcaccauugg caaggccuuc     1800

gaggcgacag ugagaggagc caaacgcaug gcugugcuug gcgacaccgc cugggauuuc     1860

ggguccgucg gaggcgcucu caauucucug gggaagggaa uccaucagau uuuuggcgcc     1920

gccuucaaaa gccuguuugg gggcaugucc ugguucucuc aaauccugau cggaacccuu     1980

cucauguggc ugggccugaa cacaaagaau gggagcauuu cccucaugug ucuggcucuu     2040

ggaggcgugc ugaucuuucu cagcacggcg guuucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10206
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10206
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg uuacgcggcg cggcuccgcg uacuauaugu accuggauag aaacgacgcu      120

ggggaggcca uuucuuuucc caccacacuu ggaaugaaua agugcuauau ccagauuaug      180

gaucugggcc auaccuguga cgccacaaug agcuacgaau gcccuaugcu cgaugagggg      240

guggaaccgg acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga      300

acgugucacc auaaaaaggg cgaggcucgg cgcucuagac gggccguuac ccugcccagc      360

cacuccacac gcaaacuuca aaccagaucu cagacauggc uggaaagccg ggaguacacc      420

aagcaucuca uccgcgugga gaauuggauu uucagaaacc cuggguuugc ucuggccgcg      480

gccgcuaucg ccuggcugcu cggauccagc acgucucaga aagucaucua ucuggugaug      540

auucuucuga ucgcucccgc cuacuccauu cggugcaucg gcguuagcaa ucgcgauuuc      600

guggaaggca ugucuggggg aacauggguc gacguggugc ucgagcacgg cggguguguc      660

accguuaugg cgcaggauaa gccuacagug gacauugaac uggugaccac cacagucucc      720

aacauggccg aggugagaag cuacugcuau gaggcuucua ucuccgauau ggccagcgac      780

ucccgguguc ccacgcaagg agaggcuuac cuugacaagc agucugauac ccaguauguu      840

ugcaaacgca cacuggugga cagaggcugg gggaauggau guggccucuu uggcaagggg      900

agccugguga ccugcgccaa auucgccugc uccaagaaaa ugacaggaaa gagcauucag      960

ccugaaaacc uggaauaccg gaucaugcuc ucuguccacg gcucccaaca uagcgggaug     1020

aucguuaaug auaccggaca cgagacggac gaaaacagag cuaaggugga gauuacaccc     1080

aauucucccc gcgcggaggc cacccugggc ggguuuggaa gccuuggccu ggauugugaa     1140

ccucggaccg gccucgacuu cuccgaucug uauuaccuua caaugaacaa uaaacauugg     1200

cugguccaca aggagugguu ucacgacauc ccccucccuu ggcaugcugg ggccgauacc     1260

ggaacacccc acuggaacaa uaaagaagcc cugguggagu ucaaggacgc ucaugccaaa     1320

agacagacgg ucgugguucu gggcucucag gaaggggcgg ugcacaccgc ucucgccgga     1380

gcccuggagg cugagaugga uggcgccaag gggcgccuua gcuccggcca ucugaaaugc     1440

cggcucaaga uggacaagcu gagacuuaaa ggagucagcu auucucugug uacagcugcc     1500

uuuaccuuca caaagauucc ggcggaaacc cuccacggca cggugaccgu ugaggugcag     1560

uacgccggga cagacggacc uugcaaaguc cccgcucaaa uggccgugga uaugcagacc     1620

cugacaccug ugggccgccu gaucaccgcu aaccccguua uuacggaauc cacagagaac     1680

agcaagauga ugcucgaacu ggacccgccu uuuggggauu cuuacaucgu caucggagug     1740

ggcgagaaaa agauuaccca ccauuggcac cgguccggga gcacaaucgg caaggccuuc     1800

gaggcgaccg ugagaggagc caaacgcaug gcuguccuug gcgacaccgc cugggauuuc     1860

ggguccgugg gaggcgcucu caauucucug gggaagggca uucaucagau cuuuggagcc     1920

gccuucaaaa gccuguuugg gggcaugucc ugguucucuc aaauucugau cggaacacuu     1980

cucauguggc ugggccugaa cgcuaagaau gggagcaucu cccucaugug ucuggcgcuu     2040

ggaggcguuc ugauuuuucu cagcacggcc gugucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10207
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10207
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagca uuacgcggcg cggcuccgcg uacuauaugu accuggauag aucugacgcu      120

gggaaggcca ucagcuuugc caccacacuu ggaguuaaca aaugccaugu gcagauuaug      180

gaucugggcc acauguguga cgcuaccaug uccuaugagu gccccaugcu cgaugaaggg      240

guggagcccg acgaugucga cuguuggugc aauacaaccu cuacaugggu gguuuacgga      300

accugucauc acaagaaagg cgaagcccgg cgcagcagac gggcugugac gcugccgucc      360

cauucuaccc gcaaacuuca aacaagaagc cagaccuggc uggagucccg ggaauauaca      420

aagcaccuca ucaaggucga gaacuggauu uuccgcaauc ccggguuugc ccugguggcg      480

guugccaucg cuuggcugcu cggaagcucu accucccaga aggugaucua ccuggucaug      540

auucuucuga ucgccccugc uuauagcauu agaugcaucg gcgugucuaa ccgggauuuc      600

guggagggca uguccggggg aacguggguc gacguugugc ucgaacacgg cgggugugug      660

acagucaugg cccaggauaa acccaccgug gacauugagc ugguuacaac caccgugagc      720

aauauggcgg aaguccgcuc uuacugcuac gaggccucca ucagcgauau ggcuuccgac      780

ucuagauguc cuacacaagg agaggccuau cuugacaagc agagcgauac gcaguacgug      840

ugcaaacgga cccugguuga ccgcggcugg gggaacggau guggccucuu uggcaagggg      900

ucccugguga caugcgcuaa auuuaccugc agcaagaaga ugacaggaaa aucuauucag      960

cccgaaaauc uggaguauag aaucaugcuc uccguccaug gcagccaaca cucugggaug     1020

aucggauacg aaaccgauga ggaccgggcc aagguggaag ucacgccuaa cagcccgaga     1080

gccgaggcua cacugggcgg guuuggaucc cuuggccugg auugugagcc ccgcaccggc     1140

cucgacuucu cugaucugua uuaccuuacc augaauaaca aacauuggcu ggugcacaag     1200

gaaugguuuc acgacauucc ucuccccugg caugcggggg ccgauacagg aaccccucac     1260

uggaauaaca aagaggcucu gguugaguuc aaggacgccc augccaaacg gcagacagug     1320

gucgugcugg gcagccagga aggggcuguu cacacggccc ucgcgggagc ucuggaagcc     1380

gagauggaug gcgccaaggg gagacuuuuc uccggccauc ugaagugccg ccucaaaaug     1440

gacaagcugc ggcuuaaagg agugagcuau ucucugugua ccgcugccuu cacauuuacc     1500

aagguccccg cugagacacu ccacggcacc gugacggugg aaguucagua cgccgggacc     1560

gacggaccgu gcaaaauccc uguccaaaug gcgguggaua ugcagacacu gacccccgug     1620

ggcagacuga uuacagccaa uccugucauc accgagucca cggaaaacag caagaugaug     1680

cucgagcugg acccucccuu cggggauucu uacauuguga ucggaguugg cgacaagaaa     1740

aucacacacc auuggcaccg cuccgggagc accauuggca aggcuuucga agccacagug     1800

cggggagcua aaagaauggc cguccuuggc gauaccgcgu gggacuuugg guccguggga     1860

ggcguuuuca auucucuggg gaagggaauc caucagauuu uuggcgccgc uuucaaaagc     1920

cucuuugggg gcauguccug guucucucaa auccugauug gaacccugcu uguguggcuc     1980

ggccugaaca caaagaacgg gagcaucucc cugacguguc ucgcccuggg aggcgucaug     2040

aucuuucuua gcaccgcugu gucugccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10208
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10208
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg uuacgcggcg cggcuccgcg uacuauaugu accuggauag aaacgacgcu      120

ggggaggcca uuucuuuucc caccacacuu ggaaugaaua agugcuauau ccagauuaug      180

gaucugggcc auauguguga cgccaccaug agcuacgaau gcccuaugcu cgaugagggg      240

guggaaccgg acgaugugga cuguuggugc aacacaaccu ccacaugggu cguguaugga      300

accugucacc auaaaaaggg cgaggcucgg cgcucuagac gggccguuac gcugcccagc      360

cacuccaccc gcaaacuuca aacaagaucu cagaccuggc uggaaagccg ggaguacaca      420

aagcaucuca uccgcgugga gaauuggauu uucagaaacc cuggguuugc ucuggccgcg      480

gccgcuaucg ccuggcugcu cggauccagc accucucaga aagucaucua ucuggugaug      540

auucuucuga ucgcucccgc cuacuccauu cggugcaucg gcguuagcaa ucgcgauuuc      600

guggaaggca ugucuggggg aacguggguc gacguggugc ucgagcacgg cggguguguc      660

acaguuaugg cgcaggauaa gccuaccgug gacauugaac uggugacaac caccgucucc      720

aacauggccg aggugagaag cuacugcuau gaggcuucua ucuccgauau ggccagcgac      780

ucccgguguc ccacacaagg agaggcuuac cuugacaagc agucugauac gcaguauguu      840

ugcaaacgca cccuggugga cagaggcugg gggaauggau guggccucuu uggcaagggg      900

agccugguca caugcgccaa auucgccugc uccaagaaaa ugaccggaaa gagcauucag      960

ccugaaaacc uggaauaccg gaucaugcuc ucugugcacg gcucccaaca uagcgggaug     1020

aucguuaaug auacaggaca cgagaccgac gaaaacagag cuaaggugga gauuacgccc     1080

aauucucccc gcgcggaggc cacacugggc ggguuuggaa gccuuggccu ggauugugaa     1140

ccucggaccg gccucgacuu cuccgaucug uauuaccuua ccaugaacaa uaaacauugg     1200

cugguccaca aggagugguu ucacgacauc ccccucccuu ggcaugcugg ggccgauaca     1260

ggaaccccgc acuggaacaa uaaagaagcc cugguggagu ucaaggacgc ucaugccaaa     1320

agacagacag ucgugguucu gggcucucag gaaggggcgg ugcacacggc ucucgccgga     1380

gcccuggagg cugagaugga uggcgccaag gggcgccuua gcuccggcca ucugaaaugc     1440

cggcucaaga uggacaagcu gagacuuaaa ggagucagcu auucucugug uaccgcugcc     1500

uuuacauuca ccaagauucc ggcggaaaca cuccacggca ccgugacggu ugaggugcag     1560

uacgccggga ccgacggacc uugcaaaguc cccgcucaaa uggccgugga uaugcagaca     1620

cugaccccug ugggccgccu gaucacagcu aaccccguua uuaccgaauc cacggagaac     1680

agcaagauga ugcucgaacu ggaucccccu uuuggggacu cuuacaucgu caucggagug     1740

ggcgagaaaa agauuacaca ccauuggcac cgguccggga gcaccaucgg caaggccuuc     1800

gaggcgacag ugagaggagc caaacgcaug gcuguccuug gcgacaccgc cugggauuuc     1860

ggguccgugg gaggcgcucu caauucucug gggaagggca uucaucagau cuuuggagcc     1920

gccuucaaaa gccuguuugg gggcaugucc ugguucucuc aaauucugau cggaacccuu     1980

cucauguggc ugggccugaa cacaaagaau gggagcaucu cccucaugug ucuggcucuu     2040

ggaggcguuc ugauuuuucu cagcacggcg gugucugccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10209
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10209
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccguu acccggcgcg gcagcgccua cuauauguac      120

cuggacagaa acgaugcugg ggaggccauc uccuucccca caacgcuugg aaugaauaaa      180

uguuauauuc agaucaugga ccugggccac augugcgaug cuaccauguc uuacgaaugu      240

ccuaugcucg acgagggggu ggaaccggau gacguggauu gcugguguaa cacaaccagc      300

acaugggucg uguauggaac cugccaucac aagaaaggcg aggcccggcg cuccagacgg      360

gcgguuacac ugcccucuca uagcacccgc aaacuucaaa cgagauccca gaccuggcug      420

gaaagccggg aguacacaaa acaccucauu cgcguggaga auuggaucuu uagaaacccu      480

ggcuucgccc uggcugccgc ugccaucgcg uggcugcucg ggucuuccac cagccagaag      540

gucauuuauc uggugaugau ccuucugauu gcccccgcuu acucuauccg guguauugga      600

guuuccaauc gcgacuuugu ggaaggcaug agcgggggaa caugggucga uaucgugcuc      660

gagcauggcg ggugcgugac cgucauggcc caggacaagc cuacgguuga uauugaacug      720

gugacaacca cagugucuaa cauggcugag gucagauccu acuguuauga ggccagcauc      780

uccgacaugg ccucugauag ccggugcccc acccaaggag aggcuuaccu ugacaaacag      840

uccgacaccc aguaugugug uaagcgcaca cugguugaua gaggcugggg caaugggugc      900

ggacucuucg gcaaagggag ccuggugacg ugcgcgaagu uugccuguuc uaaaaagaug      960

accggaaagu ccauccagcc ugaaaaccug gaauaccgga uuaugcucag cguccacggc     1020

ucucaacaca gcgggaugau cgugaaugac acaggacaug agaccgauga aaacagagcu     1080

aaaguugaga uuacacccaa uuccccgcgc gccgaggcca cccugggcgg cuucgggucu     1140

cuuggacugg acugcgaacc ucggacgggc cucgauuuua gcgaccugua uuaccuuaca     1200

augaacaaua agcacuggcu ggugcauaaa gagugguucc acgauauccc ccucccuugg     1260

cacgcugggg ccgacaccgg aacaccccau uggaacaaua aggaagcgcu ggucgaguuu     1320

aaagaugcuc acgccaagag acagaccgug gucgugcugg gcucccagga aggggccguu     1380

cauaccgcuc ucgccggcgc ucuggaggcc gagauggacg gagcgaaagg ccgccuuagc     1440

ucugggcacc ugaagugucg gcucaagaug gauaaacuga gacuuaaggg aguguccuau     1500

agccugugca cagccgcuuu cacguuuacc aaaauuccgg ccgaaacacu ccauggcacc     1560

gucacagugg agguucagua cgcugggacc gacggaccuu guaaggugcc cgcccaaaug     1620

gcggucgaca ugcagacgcu gaccccugug ggccgccuga ucacagccaa ccccgugauc     1680

accgaaucua cagagaacuc caaaaugaug cucgaacugg aucccccuuu cggggacagc     1740

uacauuguua ucggcgucgg agagaagaag auuacccacc acuggcaucg guccggcucu     1800

acgaucggga aggcuuuuga ggccacagug agaggagcua agcgcauggc cgugcuuggc     1860

gauaccgccu gggacuucgg gagcgucgga ggcgcucuca auucccuggg gaaaggcauu     1920

caccagaucu ucggagcggc cuuuaagucu cuguucggcg ggaugagcug guuuucccaa     1980

auccugauug gaacacuucu cauguggcug ggccugaaca ccaaaaaugg gagcaucucu     2040

cucaugugcc uggcucuugg aggcgugcug auuuuccucu ccaccgccgu uagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10210
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10210
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccguu acccggcgcg gcagcgccua cuauauguac      120

cuggacagaa acgaugcugg ggaggccauc uccuucccca caacgcuugg aaugaauaaa      180

uguuauauuc agaucaugga ccugggccau accugcgaug cuacaauguc uuacgaaugu      240

ccuaugcucg acgagggggu ggaaccggau gacguggauu gcugguguaa caccacaagc      300

accugggucg uguauggaac augccaccac aagaaaggcg aggcccggcg cuccagacgg      360

gcgguuaccc ugcccucuca uagcacgcgc aaacuucaaa ccagauccca gacauggcug      420

gaaagccggg aguacaccaa acaccucauu cgcguggaga auuggaucuu uagaaacccu      480

ggcuucgccc uggcugccgc ugccaucgcg uggcugcucg ggucuuccac aagccagaag      540

gucauuuauc uggugaugau ccuucugauu gcccccgcuu acucuauccg guguauugga      600

guuuccaauc gcgacuuugu ggaaggcaug agcgggggaa ccugggucga uguggugcuc      660

gagcauggcg ggugcgucac gguuauggcc caggacaagc cuacagugga uaucgaacug      720

gugaccacaa ccgucucuaa cauggcugag gugagauccu acuguuauga ggccagcauu      780

uccgacaugg ccucugauag ccggugcccc acccaaggag aggcuuaccu ugacaaacag      840

uccgacacac aguauguuug uaagcgcacg cugguggaua gaggcugggg caaugggugc      900

ggacucuucg gcaaagggag ccuggugacc ugcgcgaagu uugccuguuc uaaaaagaug      960

acaggaaagu ccauccagcc ugaaaaccug gaauaccgga ucaugcucag cguccacggc     1020

ucucaacaca gcgggaugau uguuaaugac accggacaug agacagauga aaacagagcu     1080

aaaguggaga ucacccccaa uuccccgcgc gccgaggcca cgcugggcgg cuucgggucu     1140

cuuggacugg acugcgaacc ucggacaggc cucgauuuua gcgaccugua uuaccuuacc     1200

augaacaaua agcacuggcu gguccauaaa gagugguucc acgauauucc ccucccuugg     1260

cacgcugggg ccgacaccgg aacaccccau uggaacaaua aggaagcgcu gguggaguuu     1320

aaagaugcuc acgccaagag acagaccguc gugguucugg gcucccagga aggggccgug     1380

cauacagcuc ucgccggcgc ucuggaggcc gagauggacg gagcgaaagg ccgccuuagc     1440

ucugggcacc ugaagugucg gcucaagaug gauaaacuga gacuuaaggg agucuccuau     1500

agccugugca cggccgcuuu caccuuuaca aaaaucccgg ccgaaacccu ccauggcaca     1560

gugaccguug aggugcagua cgcugggacg gacggaccuu guaagguccc cgcccaaaug     1620

gcgguggaca ugcagacccu gacaccugug ggccgccuga uuaccgccaa ccccguuauc     1680

acagaaucua ccgagaacuc caaaaugaug cucgaacugg aucccccuuu cggggacagc     1740

uacaucguca uuggcguggg agagaagaag aucacgcacc acuggcaucg guccggcucu     1800

acaauuggga aggcuuuuga ggccaccgug agaggagcua agcgcauggc cguccuuggc     1860

gauacagccu gggacuucgg gagcguggga ggcgcucuca auucccuggg gaaaggcauc     1920

caccagauuu ucggagcggc cuuuaagucu cuguucggcg ggaugagcug guuuucccaa     1980

auccugaucg gaacccuucu cauguggcug ggccugaacg cuaaaaaugg gagcauuucu     2040

cucaugugcc uggcccuugg aggcguucug aucuuccucu ccaccgccgu gagcgcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10211
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10211
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccauc acccggcgcg gcagcgccua cuauauguac      120

cuggacagau ccgaugcugg gaaagccauu ucuuucgcua caacgcuugg aguuaacaag      180

ugucacgugc agaucaugga ccugggccau augugcgaug ccaccaugag cuaugagugu      240

cccaugcucg acgaaggggu ggagcccgau gacgucgauu gcugguguaa uacaaccucc      300

acaugggugg uuuacggaac cugccaccau aaaaagggcg aagcgcggcg cucuagacgg      360

gccgugacac ugccgagcca cuccacccgc aaacuucaaa cgagaagcca gaccuggcug      420

gagucucggg aauauacaaa gcaucucauu aaggucgaga acuggaucuu ucgcaauccc      480

ggcuucgcuc ugguggccgu ugcuaucgcc uggcugcucg gguccagcac cucucagaaa      540

gugauuuacc uggucaugau ccuucugauu gcgccugccu auuccaucag auguauugga      600

gugagcaacc gggacuuugu ggagggcaug ucugggggaa caugggucga uguugugcuc      660

gaacacggcg ggugcgugac cgucauggcu caggacaagc ccacggugga uaucgagcug      720

guuacaacca caguguccaa uauggccgaa guccgcagcu acuguuacga ggcuuccauu      780

ucugacaugg ccagcgauuc cagaugcccu acccaaggag aggccuaucu ugacaaacag      840

agcgacaccc aguacgugug uaagcggaca cugguugauc gcggcugggg caacgggugc      900

ggacucuucg gcaaaggguc ucuggugacg ugcgcuaagu uuaccuguuc caagaaaaug      960

acaggaaaga gcauccagcc cgaaaaucug gaguauagaa ucaugcucuc uguccacggc     1020

agccaacauu ccgggaugau uggauacgaa accgacgagg aucgggcgaa aguggaaguc     1080

acaccuaacu cuccgagagc cgaggcuacc cugggcggcu ucgggagccu uggacuggac     1140

ugcgagcccc gcacgggccu cgauuuuucc gaccuguauu accuuacaau gaauaacaag     1200

cacuggcugg ugcauaaaga augguuccac gauaucccuc uccccuggca cgccggggcc     1260

gacaccggaa ccccucauug gaauaacaag gaggcucugg uugaauuuaa agaugcccac     1320

gcgaagcggc agacaguggu cgugcugggc agccaggagg gggcuguuca uaccgcccuc     1380

gccggcgcuc uggaagccga gauggacgga gcuaagggca gacuuuucuc ugggcaccug     1440

aaaugucgcc ucaagaugga uaaacugcgg cuuaagggag uguccuauag ccugugcaca     1500

gccgcguuua cguucaccaa aguccccgcc gagacacucc auggcaccgu gacaguggaa     1560

guucaguacg cugggaccga cggaccgugu aagauuccug uccaaauggc cguggacaug     1620

cagacgcuga cccccguggg cagacugauc acagcuaauc cugucauuac cgagucuaca     1680

gaaaacucca agaugaugcu cgagcuggau ccucccuuug gggacagcua caucgugauc     1740

ggcguuggag auaaaaagau uacccaccac uggcaucgcu ccggcucuac gaucgggaaa     1800

gccuucgaag cgacagugcg gggagccaag agaauggcug uccuuggcga caccgccugg     1860

gauuucggga gcgugggagg cguuuuuaau ucccugggga aaggcauuca ccagaucuuc     1920

ggagcugccu uuaagucucu cuucggcggg augagcuggu uuucccaaau ucugaucgga     1980

acacugcuug uguggcucgg ccugaacacc aagaacggga gcaucucucu gaccugccuc     2040

gcccugggag gcgucaugau uuuccuuucc acagcuguga gcgcgugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10212
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10212
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccguu acccggcgcg gcagcgccua cuauauguac      120

cuggacagaa acgaugcugg ggaggccauc uccuucccca caacgcuugg aaugaauaaa      180

uguuauauuc agaucaugga ccugggccac augugcgaug cuaccauguc uuacgaaugu      240

ccuaugcucg acgagggggu ggaaccggau gacguggauu gcugguguaa cacaaccagc      300

acaugggucg uguauggaac cugccaucac aagaaaggcg aggcccggcg cuccagacgg      360

gcgguuacac ugcccucuca uagcacccgc aaacuucaaa cgagauccca gaccuggcug      420

gaaagccggg aguacacaaa acaccucauu cgcguggaga auuggaucuu uagaaacccu      480

ggcuucgccc uggcugccgc ugccaucgcg uggcugcucg ggucuuccac cagccagaag      540

gucauuuauc uggugaugau ccuucugauu gcccccgcuu acucuauccg guguauugga      600

guuuccaauc gcgacuuugu ggaaggcaug agcgggggaa caugggucga uguggugcuc      660

gagcauggcg ggugcgucac cguuauggcc caggacaagc cuacggugga uaucgaacug      720

gugacaacca cagucucuaa cauggcugag gugagauccu acuguuauga ggccagcauu      780

uccgacaugg ccucugauag ccggugcccc acccaaggag aggcuuaccu ugacaaacag      840

uccgacaccc aguauguuug uaagcgcaca cugguggaua gaggcugggg caaugggugc      900

ggacucuucg gcaaagggag ccuggucacg ugcgcgaagu uugccuguuc uaaaaagaug      960

accggaaagu ccauccagcc ugaaaaccug gaauaccgga ucaugcucag cgugcacggc     1020

ucucaacaca gcgggaugau uguuaaugac acaggacaug agaccgauga aaacagagcu     1080

aaaguggaga ucacacccaa uuccccgcgc gccgaggcca cccugggcgg cuucgggucu     1140

cuuggacugg acugcgaacc ucggacgggc cucgauuuua gcgaccugua uuaccuuaca     1200

augaacaaua agcacuggcu gguccauaaa gagugguucc acgauauucc ccucccuugg     1260

cacgcugggg ccgacaccgg aacaccccau uggaacaaua aggaagcgcu gguggaguuu     1320

aaagaugcuc acgccaagag acagaccguc gugguucugg gcucccagga aggggccgug     1380

cauaccgcuc ucgccggcgc ucuggaggcc gagauggacg gagcgaaagg ccgccuuagc     1440

ucugggcacc ugaagugucg gcucaagaug gauaaacuga gacuuaaggg agucuccuau     1500

agccugugca cagccgcuuu cacguuuacc aaaaucccgg ccgaaacacu ccauggcacc     1560

gugacaguug aggugcagua cgcugggacc gacggaccuu guaagguccc cgcccaaaug     1620

gcgguggaca ugcagacgcu gaccccugug ggccgccuga uuacagccaa ccccguuauc     1680

accgaaucua cagagaacuc caaaaugaug cucgaacugg aucccccuuu cggggacagc     1740

uacaucguca uuggcguggg agagaagaag aucacccacc acuggcaucg guccggcucu     1800

acgauuggga aggcuuuuga ggccacagug agaggagcua agcgcauggc cguccuuggc     1860

gauaccgccu gggacuucgg gagcguggga ggcgcucuca auucccuggg gaaaggcauc     1920

caccagauuu ucggagcggc cuuuaagucu cuguucggcg ggaugagcug guuuucccaa     1980

auccugaucg gaacacuucu cauguggcug ggccugaaca ccaaaaaugg gagcauuucu     2040

cucaugugcc uggcucuugg aggcguucug aucuuccucu ccaccgccgu gagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10213
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10213
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gugaccagac ggggaagcgc uuacuauaug      120

uaccuggacc gcaacgaugc cggcgaagcc auuuccuucc cuacaacgcu ugggaugaau      180

aagugcuaua uccaaauuau ggaccuggga cacaugugug augcuaccau gucuuacgag      240

ugcccgaugc ucgacgaagg cguggagccc gaugacgucg auuguuggug caacacaacc      300

agcacauggg ugguuuaugg gaccugucau cacaaaaagg gagaagccag acggucccgc      360

agagcuguga cacugccuuc ucauagcacc cggaaacuuc agacgcgcuc ccagaccugg      420

cuggagagca gagaguacac aaagcaccuc auccgggucg aaaauuggau cuuucgcaac      480

cccggcuucg cccuggcggc cgcugccauu gcuuggcugc ucgggucuuc caccagccag      540

aaagugaucu aucugguuau gauucuucug aucgccccug cguacucuau uagaugcauc      600

ggggugucca aucgggacuu ugucgaggga augagcggcg ggacaugggu ggauauugug      660

cucgaacaug gaggcugugu caccguuaug gcccaagaca agcccacggu ggauaucgag      720

cuggugacaa ccacagucuc uaacauggcu gaggugcgcu ccuacugcua ugaagccagc      780

aucuccgaca uggcuucuga uagcagaugu ccuacccagg gggaggccua ccuugacaag      840

caguccgaca cccaguaugu uugcaaacgg acacuggugg aucgcggaug gggcaauggc      900

uguggccucu ucggaaaggg cagccugguc acgugcgcca aauuugcuug cucuaagaaa      960

augaccggga aguccauuca accggaaaac cuggaguaca gaaucaugcu cagcgugcac     1020

ggaucucagc acagcggcau gauuguuaau gacacagggc augaaaccga ugagaaccgg     1080

gcgaaggugg agaucacacc caauuccccu agagccgaag cuacccuggg aggcuucggc     1140

ucucuugggc uggacuguga gccccgcacg ggacucgauu uuagcgaccu guauuaccuu     1200

acaaugaaca auaaacacug gcugguccau aaggaauggu uccacgauau uccucucccc     1260

uggcacgccg gcgccgacac cgggaccccg cauuggaaca auaaagaggc ucugguggaa     1320

uuuaaggaug cccacgcgaa acggcagaca gucgugguuc ugggauccca ggagggcgcu     1380

gugcauaccg cccucgccgg ggcucuggag gccgaaaugg acggcgcuaa gggaagacuu     1440

agcucuggcc accugaaaug ccgccucaag auggauaagc ugcggcuuaa aggggucucc     1500

uauagccugu guacagccgc guucacguuu accaagaucc cugccgagac acuccaugga     1560

accgugacag uugaagugca auacgcuggc accgacgggc ccugcaaagu cccugcccag     1620

auggcugugg acaugcagac gcugaccccc gugggaagac ugaucacagc caaccccguu     1680

auuaccgagu cuacagaaaa cuccaagaug augcucgagc uggauccucc guucggcgac     1740

agcuacaucg ucauuggggu gggcgagaaa aagaucaccc accacuggca ucgcuccgga     1800

ucuacgcuug gcaaggcguu cagcacaacc cugaaagggg cccaacggcu cgcugcccug     1860

ggagauacag cuugggacuu uggcuccauu gggggagugu ucaauucuau cggcagagcc     1920

guccaccagg uguuuggggg cgccuuccgc acccuguuug gaggcaugag cuggaucacc     1980

caggggcuua ugggagcucu ccugcugugg augggcguua acgcgcggga uagauccauu     2040

gcccucgcuu uccuggccac agggggagug cuugucuuuc uggccacgaa ugugcaugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10214
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10214
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gugaccagac ggggaagcgc uuacuauaug      120

uaccuggacc gcaacgaugc cggcgaagcc auuuccuucc cuacaacgcu ugggaugaau      180

aagugcuaua uccaaauuau ggaccuggga cacaccugug augcuacaau gucuuacgag      240

ugcccgaugc ucgacgaagg cguggagccc gaugacgucg auuguuggug caacaccaca      300

agcaccuggg ugguuuaugg gacaugucau cacaaaaagg gagaagccag acggucccgc      360

agagcuguga cccugccuuc ucauagcacg cggaaacuuc agacccgcuc ccagacaugg      420

cuggagagca gagaguacac caagcaccuc auccgggucg aaaauuggau cuuucgcaac      480

cccggcuucg cccuggcggc cgcugccauu gcuuggcugc ucgggucuuc cacaagccag      540

aaagugaucu aucugguuau gauucuucug aucgccccug cguacucuau uagaugcauc      600

ggggugucca aucgggacuu ugucgaggga augagcggcg ggaccugggu ggaugugguc      660

cucgaacaug gaggcugugu uacggugaug gcccaagaca agcccacagu ggauauugag      720

cuggucacca caaccguguc uaacauggcu gagguucgcu ccuacugcua ugaagccagc      780

aucuccgaca uggcuucuga uagcagaugu ccuacccagg gggaggccua ccuugacaag      840

caguccgaca cacaguaugu gugcaaacgg acgcuggucg aucgcggaug gggcaauggc      900

uguggccucu ucggaaaggg cagccuggug accugcgcca aauuugcuug cucuaagaaa      960

augacaggga aguccaucca accggaaaac cuggaguaca gaauuaugcu cagcguucac     1020

ggaucucagc acagcggcau gaucgugaau gacaccgggc augaaacaga ugagaaccgg     1080

gcgaaggucg agauuacccc caauuccccu agagccgaag cuacgcuggg aggcuucggc     1140

ucucuugggc uggacuguga gccccgcaca ggacucgauu uuagcgaccu guauuaccuu     1200

accaugaaca auaaacacug gcuggugcau aaggaauggu uccacgauau cccucucccc     1260

uggcacgccg gcgccgacac cgggacaccg cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggaug cccacgcgaa acggcagacc gugguugugc ugggauccca ggagggcgcu     1380

guccauacag cccucgccgg ggcucuggag gccgaaaugg acggcgcuaa gggaagacuu     1440

agcucuggcc accugaaaug ccgccucaag auggauaagc ugcggcuuaa aggggugucc     1500

uauagccugu guacggccgc guucaccuuu acaaagauuc cugccgagac ccuccaugga     1560

acaguuaccg uggaagucca auacgcuggc acggacgggc ccugcaaagu gccugcccag     1620

auggcugugg acaugcagac ccugacaccc guuggaagac ugaucaccgc caaccccguc     1680

aucacagagu cuaccgaaaa cuccaagaug augcucgagc uggauccucc guucggcgac     1740

agcuacauug ugaucggggu gggcgagaaa aagauuacgc accacuggca ucgcuccgga     1800

ucuacacuug gcaaggcguu cagcaccaca cugaaagggg cccaacggcu cgcugcccug     1860

ggagauaccg cuugggacuu uggcuccauc gggggagucu ucaauucuau uggcagagcc     1920

gugcaccagg uuuuuggggg cgccuuccgc acccuguuug gaggcaugag cuggaucaca     1980

caggggcuua ugggagcucu ccugcugugg augggcguga acgcgcggga uagauccauc     2040

gcccucgcuu uccuggccac cgggggaguc cuuguguuuc uggccacgaa uguucaugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10215
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10215
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg auuaccagac ggggaagcgc uuacuauaug      120

uaccuggacc gcuccgaugc cggcaaggcc aucucuuucg cuacaacgcu uggggugaac      180

aaaugccacg ugcaaauuau ggaccuggga cauaugugug augccaccau gagcuaugaa      240

ugcccuaugc ucgacgaggg cgucgaaccg gaugacgugg auuguuggug caauacaacc      300

uccacauggg uuguguacgg gaccugucac cauaagaaag gagaggcuag acggucucgc      360

agagccguca cacugcccag ccacuccacc cggaagcucc agacgcgcag ccagaccugg      420

cuggaaucua gagaguauac aaaacaucuc aucaaggugg agaacuggau cuuucggaau      480

ccuggcuucg cgcugguugc cguggcuauu gccuggcugc ucggcuccag caccucucag      540

aaggucaucu accuggugau gauucuucug aucgcucccg ccuauuccau ucgcugcauc      600

ggggugagca acagagacuu ugucgaagga augucuggcg ggacaugggu ugauguggug      660

cucgagcacg gaggcugugu caccgugaug gcgcaagaca aaccuacggu ugauauugaa      720

cuggugacaa ccacagucuc caauauggcc gaggugcgga gcuacugcua cgaggcuucc      780

aucucugaca uggccagcga uucccgcugu cccacccagg gggaggcuua ucuugacaag      840

cagagcgaca cccaguacgu uugcaaaaga acacuggugg aucggggaug gggcaacggc      900

ugugggcuuu ucggaaaggg cucucugguc acgugcgcca aauuuaccug cuccaagaag      960

augacaggga aaagcaucca accugaaaau cuggaauauc gcauuaugcu cucugugcac     1020

ggaagccagc auuccggcau gaucggguac gagaccgacg aagauagagc caaggucgag     1080

gugacaccga acucuccccg ggcugaggcg acccugggag gcuucggcag ccuugggcug     1140

gacugugaac cuagaacggg acucgauuuu uccgaccugu auuaccuuac aaugaauaac     1200

aaacacuggc ugguucauaa ggagugguuc cacgauauuc cccucccuug gcacgccggc     1260

gcugacaccg ggacacccca uuggaauaac aaagaagccc ugguggaguu uaaggaugcc     1320

cacgcuaaac gccagaccgu cgugguucug ggaagccagg aaggcgccgu gcauaccgcg     1380

cucgcugggg cccuggaggc cgagauggac ggcgcuaagg gacggcuuuu cucuggccac     1440

cugaagugca gacucaaaau ggauaagcug cgccuuaaag gggucuccua uagccugugu     1500

acagccgcuu uuacguucac caaggugccg gccgaaacac uccauggaac cgugacaguu     1560

gagguccaau acgcgggcac cgacgggccu ugcaaaaucc ccgugcagau ggccguggac     1620

augcagacgc ugaccccugu cggacggcug auuacagcua aucccgugau caccgaaucu     1680

acagagaacu ccaagaugau gcucgaacug gaucccccuu ucggcgacag cuacaucguu     1740

auuggggugg gcgauaagaa aaucacccac cacuggcaua gauccggauc uacgcuuggc     1800

aaggccuuua gcacaacccu gaaaggggcu caacgccucg ccgcgcuggg agacacagcc     1860

ugggauuucg gcuccauugg gggagucuuu aauucuaucg gccgggcugu gcaccagguu     1920

uucgggggcg ccuuuagaac ccuguucgga ggcaugagcu ggauuaccca ggggcuuaug     1980

ggagcucucc ugcuguggau gggcgugaac gcccgcgacc gguccaucgc ccucgcuuuu     2040

cuggcgacag ggggaguccu uguguuccug gccacgaacg uucaugcuug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10216
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10216
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gugaccagac ggggaagcgc uuacuauaug      120

uaccuggacc gcaacgaugc cggcgaagcc auuuccuucc cuacaacgcu ugggaugaau      180

aagugcuaua uccaaauuau ggaccuggga cacaugugug augcuaccau gucuuacgag      240

ugcccgaugc ucgacgaagg cguggagccc gaugacgucg auuguuggug caacacaacc      300

agcacauggg ugguuuaugg gaccugucau cacaaaaagg gagaagccag acggucccgc      360

agagcuguga cacugccuuc ucauagcacc cggaaacuuc agacgcgcuc ccagaccugg      420

cuggagagca gagaguacac aaagcaccuc auccgggucg aaaauuggau cuuucgcaac      480

cccggcuucg cccuggcggc cgcugccauu gcuuggcugc ucgggucuuc caccagccag      540

aaagugaucu aucugguuau gauucuucug aucgccccug cguacucuau uagaugcauc      600

ggggugucca aucgggacuu ugucgaggga augagcggcg ggacaugggu ggaugugguc      660

cucgaacaug gaggcugugu uaccgugaug gcccaagaca agcccacggu ggauauugag      720

cuggucacaa ccacaguguc uaacauggcu gagguucgcu ccuacugcua ugaagccagc      780

aucuccgaca uggcuucuga uagcagaugu ccuacccagg gggaggccua ccuugacaag      840

caguccgaca cccaguaugu gugcaaacgg acacuggucg aucgcggaug gggcaauggc      900

uguggccucu ucggaaaggg cagccuggug acgugcgcca aauuugcuug cucuaagaaa      960

augaccggga aguccaucca accggaaaac cuggaguaca gaauuaugcu cagcguucac     1020

ggaucucagc acagcggcau gaucgugaau gacacagggc augaaaccga ugagaaccgg     1080

gcgaaggucg agauuacacc caauuccccu agagccgaag cuacccuggg aggcuucggc     1140

ucucuugggc uggacuguga gccccgcacg ggacucgauu uuagcgaccu guauuaccuu     1200

acaaugaaca auaaacacug gcuggugcau aaggaauggu uccacgauau cccucucccc     1260

uggcacgccg gcgccgacac cgggaccccg cauuggaaca auaaagaggc ucuggucgaa     1320

uuuaaggaug cccacgcgaa acggcagaca gugguugugc ugggauccca ggagggcgcu     1380

guccauaccg cccucgccgg ggcucuggag gccgaaaugg acggcgcuaa gggaagacuu     1440

agcucuggcc accugaaaug ccgccucaag auggauaagc ugcggcuuaa aggggugucc     1500

uauagccugu guacagccgc guucacguuu accaagauuc cugccgagac acuccaugga     1560

accguuacag uggaagucca auacgcuggc accgacgggc ccugcaaagu gccugcccag     1620

auggcugugg acaugcagac gcugaccccc guuggaagac ugaucacagc caaccccguc     1680

aucaccgagu cuacagaaaa cuccaagaug augcucgagc uggauccucc guucggcgac     1740

agcuacauug ugaucggggu gggcgagaaa aagauuaccc accacuggca ucgcuccgga     1800

ucuacgcuug gcaaggcguu cagcacaacc cugaaagggg cccaacggcu cgcugcccug     1860

ggagauacag cuugggacuu uggcuccauc gggggagucu ucaauucuau uggcagagcc     1920

gugcaccagg uuuuuggggg cgccuuccgc acccuguuug gaggcaugag cuggaucacc     1980

caggggcuua ugggagcucu ccugcugugg augggcguga acgcgcggga uagauccauc     2040

gcccucgcuu uccuggccac agggggaguc cuuguguuuc uggccacgaa uguucaugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10217
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10217
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg uuacgcggcg cggcuccgcg uacuauaugu accuggauag aaacgacgcu      120

ggggaggcca uuucuuuucc caccacacuu ggaaugaaua agugcuauau ccagauuaug      180

gaucugggcc auauguguga cgccaccaug agcuacgaau gcccuaugcu cgaugagggg      240

guggaaccgg acgaugugga cuguuggugc aacacaaccu ccacaugggu cguguaugga      300

accugucacc auaaaaaggg cgaggcucgg cgcucuagac gggccguuac gcugcccagc      360

cacuccaccc gcaaacuuca aacaagaucu cagaccuggc uggaaagccg ggaguacaca      420

aagcaucuca uccgcgugga gaauuggauu uucagaaacc cuggguuugc ucuggccgcg      480

gccgcuaucg ccuggcugcu cggauccagc accucucaga aagucaucua ucuggugaug      540

auucuucuga ucgcucccgc cuacuccauu cggugcaucg gcguuagcaa ucgcgauuuc      600

guggaaggca ugucuggggg aacguggguc gacauugugc ucgagcacgg cgggugugug      660

acagucaugg cgcaggauaa gccuaccguu gacaucgaac uggugacaac caccgugucc      720

aacauggccg aggucagaag cuacugcuau gaggcuucua uuuccgauau ggccagcgac      780

ucccgguguc ccacacaagg agaggcuuac cuugacaagc agucugauac gcaguaugug      840

ugcaaacgca cccugguuga cagaggcugg gggaauggau guggccucuu uggcaagggg      900

agccugguga caugcgccaa auucgccugc uccaagaaaa ugaccggaaa gagcauccag      960

ccugaaaacc uggaauaccg gaucaugcuc ucuguccacg gcucccaaca uagcgggaug     1020

auugugaaug auacaggaca cgagaccgac gaaaacagag cuaagguuga gaucacgccc     1080

aauucucccc gcgcggaggc cacacugggc ggguuuggaa gccuuggccu ggauugugaa     1140

ccucggaccg gccucgacuu cuccgaucug uauuaccuua ccaugaacaa uaaacauugg     1200

cuggugcaca aggagugguu ucacgacauu ccccucccuu ggcaugcugg ggccgauaca     1260

ggaaccccgc acuggaacaa uaaagaagcc cuggucgagu ucaaggacgc ucaugccaaa     1320

agacagacag uggucgugcu gggcucucag gaaggggcgg uucacacggc ucucgccgga     1380

gcccuggagg cugagaugga uggcgccaag gggcgccuua gcuccggcca ucugaaaugc     1440

cggcucaaga uggacaagcu gagacuuaaa ggagugagcu auucucugug uaccgcugcc     1500

uuuacauuca ccaagauccc ggcggaaaca cuccacggca ccgucacggu ggagguucag     1560

uacgccggga ccgacggacc uugcaaagug cccgcucaaa uggccgucga uaugcagaca     1620

cugaccccug ugggccgccu gauuacagcu aaccccguga ucaccgaauc cacggagaac     1680

agcaagauga ugcucgaacu ggaucccccu uuuggggacu cuuacaucgu uauuggaguc     1740

ggcgagaaaa agaucacaca ccauuggcac cgguccggga gcacccuugg caaggccuuc     1800

uccacaaccc ugaaaggagc gcagagacuc gccgcucugg gcgacaccgc cugggauuuc     1860

gggucuauug gaggcguguu uaauagcauc gggcgcgcug ugcaucaagu cuucggaggc     1920

gccuuucgga cacuguucgg gggcaugucc uggauuacgc agggacuuau gggcgcccuc     1980

cugcugugga ugggggugaa cgcuagagac cgcucuaucg cgcucgccuu ucuggcuacc     2040

ggaggcguuc uuguguuccu ggccacaaau guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10218
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10218
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg uuacgcggcg cggcuccgcg uacuauaugu accuggauag aaacgacgcu      120

ggggaggcca uuucuuuucc caccacacuu ggaaugaaua agugcuauau ccagauuaug      180

gaucugggcc auaccuguga cgccacaaug agcuacgaau gcccuaugcu cgaugagggg      240

guggaaccgg acgaugugga cuguuggugc aacaccacau ccaccugggu cguguaugga      300

acgugucacc auaaaaaggg cgaggcucgg cgcucuagac gggccguuac ccugcccagc      360

cacuccacac gcaaacuuca aaccagaucu cagacauggc uggaaagccg ggaguacacc      420

aagcaucuca uccgcgugga gaauuggauu uucagaaacc cuggguuugc ucuggccgcg      480

gccgcuaucg ccuggcugcu cggauccagc acgucucaga aagucaucua ucuggugaug      540

auucuucuga ucgcucccgc cuacuccauu cggugcaucg gcguuagcaa ucgcgauuuc      600

guggaaggca ugucuggggg aacauggguc gacguggugc ucgagcacgg cggguguguc      660

accguuaugg cgcaggauaa gccuacagug gacauugaac uggugaccac cacagucucc      720

aacauggccg aggugagaag cuacugcuau gaggcuucua ucuccgauau ggccagcgac      780

ucccgguguc ccacgcaagg agaggcuuac cuugacaagc agucugauac ccaguauguu      840

ugcaaacgca cacuggugga cagaggcugg gggaauggau guggccucuu uggcaagggg      900

agccugguga ccugcgccaa auucgccugc uccaagaaaa ugacaggaaa gagcauucag      960

ccugaaaacc uggaauaccg gaucaugcuc ucuguccacg gcucccaaca uagcgggaug     1020

aucguuaaug auaccggaca cgagacggac gaaaacagag cuaaggugga gauuacaccc     1080

aauucucccc gcgcggaggc cacccugggc ggguuuggaa gccuuggccu ggauugugaa     1140

ccucggaccg gccucgacuu cuccgaucug uauuaccuua caaugaacaa uaaacauugg     1200

cugguccaca aggagugguu ucacgacauc ccccucccuu ggcaugcugg ggccgauacc     1260

ggaacacccc acuggaacaa uaaagaagcc cugguggagu ucaaggacgc ucaugccaaa     1320

agacagacgg ucgugguucu gggcucucag gaaggggcgg ugcacaccgc ucucgccgga     1380

gcccuggagg cugagaugga uggcgccaag gggcgccuua gcuccggcca ucugaaaugc     1440

cggcucaaga uggacaagcu gagacuuaaa ggagucagcu auucucugug uacagcugcc     1500

uuuaccuuca caaagauucc ggcggaaacc cuccacggca cggugaccgu ugaggugcag     1560

uacgccggga cagacggacc uugcaaaguc cccgcucaaa uggccgugga uaugcagacc     1620

cugacaccug ugggccgccu gaucaccgcu aaccccguua uuacggaauc cacagagaac     1680

agcaagauga ugcucgaacu ggacccgccu uuuggggauu cuuacaucgu caucggagug     1740

ggcgagaaaa agauuaccca ccauuggcac cgguccggga gcacacuugg caaggccuuc     1800

uccaccaccc ugaaaggagc gcagagacuc gccgcucugg gcgacacagc cugggauuuc     1860

gggucuaucg gaggcguguu uaauagcauu gggcgcgcug uccaucaagu guucggaggc     1920

gccuuucgga cgcuguucgg gggcaugucc uggaucaccc agggacuuau gggcgcccuc     1980

cugcugugga uggggguuaa cgcuagagac cgcucuauug cgcucgccuu ucuggcuaca     2040

ggaggcgugc uugucuuccu ggccaccaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10219
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10219
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagca uuacgcggcg cggcuccgcg uacuauaugu accuggauag aucugacgcu      120

gggaaggcca ucagcuuugc caccacacuu ggaguuaaca aaugccaugu gcagauuaug      180

gaucugggcc acauguguga cgcuaccaug uccuaugagu gccccaugcu cgaugaaggg      240

guggagcccg acgaugucga cuguuggugc aauacaaccu cuacaugggu gguuuacgga      300

accugucauc acaagaaagg cgaagcccgg cgcagcagac gggcugugac gcugccgucc      360

cauucuaccc gcaaacuuca aacaagaagc cagaccuggc uggagucccg ggaauauaca      420

aagcaccuca ucaaggucga gaacuggauu uuccgcaauc ccggguuugc ccugguggcg      480

guugccaucg cuuggcugcu cggaagcucu accucccaga aggugaucua ccuggucaug      540

auucuucuga ucgccccugc uuauagcauu agaugcaucg gcgugucuaa ccgggauuuc      600

guggagggca uguccggggg aacguggguc gacguugugc ucgaacacgg cgggugugug      660

acagucaugg cccaggauaa acccaccgug gacauugagc ugguuacaac caccgugagc      720

aauauggcgg aaguccgcuc uuacugcuac gaggccucca ucagcgauau ggcuuccgac      780

ucuagauguc cuacacaagg agaggccuau cuugacaagc agagcgauac gcaguacgug      840

ugcaaacgga cccugguuga ccgcggcugg gggaacggau guggccucuu uggcaagggg      900

ucccugguga caugcgcuaa auuuaccugc agcaagaaga ugacaggaaa aucuauucag      960

cccgaaaauc uggaguauag aaucaugcuc uccguccaug gcagccaaca cucugggaug     1020

aucggauacg aaaccgauga ggaccgggcc aagguggaag ucacgccuaa cagcccgaga     1080

gccgaggcua cacugggcgg guuuggaucc cuuggccugg auugugagcc ccgcaccggc     1140

cucgacuucu cugaucugua uuaccuuacc augaauaaca aacauuggcu ggugcacaag     1200

gaaugguuuc acgacauucc ucuccccugg caugcggggg ccgauacagg aaccccucac     1260

uggaauaaca aagaggcucu gguugaguuc aaggacgccc augccaaacg gcagacagug     1320

gucgugcugg gcagccagga aggggcuguu cacacggccc ucgcgggagc ucuggaagcc     1380

gagauggaug gcgccaaggg gagacuuuuc uccggccauc ugaagugccg ccucaaaaug     1440

gacaagcugc ggcuuaaagg agugagcuau ucucugugua ccgcugccuu cacauuuacc     1500

aagguccccg cugagacacu ccacggcacc gugacggugg aaguucagua cgccgggacc     1560

gacggaccgu gcaaaauccc uguccaaaug gcgguggaua ugcagacacu gacccccgug     1620

ggcagacuga uuacagccaa uccugucauc accgagucca cggaaaacag caagaugaug     1680

cucgagcugg acccucccuu cggggauucu uacauuguga ucggaguugg cgacaagaaa     1740

aucacacacc auuggcaccg cuccgggagc acccuuggca aggcuuucuc cacaacccug     1800

aaaggagccc agcggcucgc ugcccugggc gauaccgcgu gggacuuugg gucuauugga     1860

ggcguguuca auagcaucgg gagagccguc caucaagugu uuggaggcgc uuuccgcaca     1920

cuguuugggg gcauguccug gauuacgcag ggacuuaugg gcgcccuccu gcuguggaug     1980

gggguuaacg cucgggauag aucuaucgcc cucgccuucc uggcuaccgg aggcgugcuu     2040

gucuuucugg cgacaaacgu gcacgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10220
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10220
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg uuacgcggcg cggcuccgcg uacuauaugu accuggauag aaacgacgcu      120

ggggaggcca uuucuuuucc caccacacuu ggaaugaaua agugcuauau ccagauuaug      180

gaucugggcc auauguguga cgccaccaug agcuacgaau gcccuaugcu cgaugagggg      240

guggaaccgg acgaugugga cuguuggugc aacacaaccu ccacaugggu cguguaugga      300

accugucacc auaaaaaggg cgaggcucgg cgcucuagac gggccguuac gcugcccagc      360

cacuccaccc gcaaacuuca aacaagaucu cagaccuggc uggaaagccg ggaguacaca      420

aagcaucuca uccgcgugga gaauuggauu uucagaaacc cuggguuugc ucuggccgcg      480

gccgcuaucg ccuggcugcu cggauccagc accucucaga aagucaucua ucuggugaug      540

auucuucuga ucgcucccgc cuacuccauu cggugcaucg gcguuagcaa ucgcgauuuc      600

guggaaggca ugucuggggg aacguggguc gacguggugc ucgagcacgg cggguguguc      660

acaguuaugg cgcaggauaa gccuaccgug gacauugaac uggugacaac caccgucucc      720

aacauggccg aggugagaag cuacugcuau gaggcuucua ucuccgauau ggccagcgac      780

ucccgguguc ccacacaagg agaggcuuac cuugacaagc agucugauac gcaguauguu      840

ugcaaacgca cccuggugga cagaggcugg gggaauggau guggccucuu uggcaagggg      900

agccugguca caugcgccaa auucgccugc uccaagaaaa ugaccggaaa gagcauucag      960

ccugaaaacc uggaauaccg gaucaugcuc ucugugcacg gcucccaaca uagcgggaug     1020

aucguuaaug auacaggaca cgagaccgac gaaaacagag cuaaggugga gauuacgccc     1080

aauucucccc gcgcggaggc cacacugggc ggguuuggaa gccuuggccu ggauugugaa     1140

ccucggaccg gccucgacuu cuccgaucug uauuaccuua ccaugaacaa uaaacauugg     1200

cugguccaca aggagugguu ucacgacauc ccccucccuu ggcaugcugg ggccgauaca     1260

ggaaccccgc acuggaacaa uaaagaagcc cugguggagu ucaaggacgc ucaugccaaa     1320

agacagacag ucgugguucu gggcucucag gaaggggcgg ugcacacggc ucucgccgga     1380

gcccuggagg cugagaugga uggcgccaag gggcgccuua gcuccggcca ucugaaaugc     1440

cggcucaaga uggacaagcu gagacuuaaa ggagucagcu auucucugug uaccgcugcc     1500

uuuacauuca ccaagauucc ggcggaaaca cuccacggca ccgugacggu ugaggugcag     1560

uacgccggga ccgacggacc uugcaaaguc cccgcucaaa uggccgugga uaugcagaca     1620

cugaccccug ugggccgccu gaucacagcu aaccccguua uuaccgaauc cacggagaac     1680

agcaagauga ugcucgaacu ggaucccccu uuuggggacu cuuacaucgu caucggagug     1740

ggcgagaaaa agauuacaca ccauuggcac cgguccggga gcacccuugg caaggccuuc     1800

uccacaaccc ugaaaggagc gcagagacuc gccgcucugg gcgacaccgc cugggauuuc     1860

gggucuaucg gaggcguguu uaauagcauu gggcgcgcug uccaucaagu guucggaggc     1920

gccuuucgga cacuguucgg gggcaugucc uggaucacgc agggacuuau gggcgcccuc     1980

cugcugugga uggggguuaa cgcuagagac cgcucuauug cgcucgccuu ucuggcuacc     2040

ggaggcgugc uugucuuccu ggccacaaau gugcacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10221
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10221
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccguu acccggcgcg gcagcgccua cuauauguac      120

cuggacagaa acgaugcugg ggaggccauc uccuucccca caacgcuugg aaugaauaaa      180

uguuauauuc agaucaugga ccugggccac augugcgaug cuaccauguc uuacgaaugu      240

ccuaugcucg acgagggggu ggaaccggau gacguggauu gcugguguaa cacaaccagc      300

acaugggucg uguauggaac cugccaucac aagaaaggcg aggcccggcg cuccagacgg      360

gcgguuacac ugcccucuca uagcacccgc aaacuucaaa cgagauccca gaccuggcug      420

gaaagccggg aguacacaaa acaccucauu cgcguggaga auuggaucuu uagaaacccu      480

ggcuucgccc uggcugccgc ugccaucgcg uggcugcucg ggucuuccac cagccagaag      540

gucauuuauc uggugaugau ccuucugauu gcccccgcuu acucuauccg guguauugga      600

guuuccaauc gcgacuuugu ggaaggcaug agcgggggaa caugggucga uaucgugcuc      660

gagcauggcg ggugcgugac cgucauggcc caggacaagc cuacgguuga uauugaacug      720

gugacaacca cagugucuaa cauggcugag gucagauccu acuguuauga ggccagcauc      780

uccgacaugg ccucugauag ccggugcccc acccaaggag aggcuuaccu ugacaaacag      840

uccgacaccc aguaugugug uaagcgcaca cugguugaua gaggcugggg caaugggugc      900

ggacucuucg gcaaagggag ccuggugacg ugcgcgaagu uugccuguuc uaaaaagaug      960

accggaaagu ccauccagcc ugaaaaccug gaauaccgga uuaugcucag cguccacggc     1020

ucucaacaca gcgggaugau cgugaaugac acaggacaug agaccgauga aaacagagcu     1080

aaaguugaga uuacacccaa uuccccgcgc gccgaggcca cccugggcgg cuucgggucu     1140

cuuggacugg acugcgaacc ucggacgggc cucgauuuua gcgaccugua uuaccuuaca     1200

augaacaaua agcacuggcu ggugcauaaa gagugguucc acgauauccc ccucccuugg     1260

cacgcugggg ccgacaccgg aacaccccau uggaacaaua aggaagcgcu ggucgaguuu     1320

aaagaugcuc acgccaagag acagaccgug gucgugcugg gcucccagga aggggccguu     1380

cauaccgcuc ucgccggcgc ucuggaggcc gagauggacg gagcgaaagg ccgccuuagc     1440

ucugggcacc ugaagugucg gcucaagaug gauaaacuga gacuuaaggg aguguccuau     1500

agccugugca cagccgcuuu cacguuuacc aaaauuccgg ccgaaacacu ccauggcacc     1560

gucacagugg agguucagua cgcugggacc gacggaccuu guaaggugcc cgcccaaaug     1620

gcggucgaca ugcagacgcu gaccccugug ggccgccuga ucacagccaa ccccgugauc     1680

accgaaucua cagagaacuc caaaaugaug cucgaacugg aucccccuuu cggggacagc     1740

uacauuguua ucggcgucgg agagaagaag auuacccacc acuggcaucg guccggcucu     1800

acgcuuggga aggcuuuuag cacaacccug aagggagccc agagacucgc ugcccugggc     1860

gauacagccu gggacuucgg guccaucgga ggcguguuca auucuauugg gcgcgcugug     1920

caccaagucu uuggcggagc guuccggacc cuguuuggcg ggaugagcug gaucacccag     1980

ggacuuaugg gcgcccuccu gcuguggaug ggggugaacg cuagagaucg cuccaucgcc     2040

cucgccuucc uggcuacagg aggcguucuu guguuucugg ccacgaaugu ccaugcguga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10222
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10222
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccguu acccggcgcg gcagcgccua cuauauguac      120

cuggacagaa acgaugcugg ggaggccauc uccuucccca caacgcuugg aaugaauaaa      180

uguuauauuc agaucaugga ccugggccau accugcgaug cuacaauguc uuacgaaugu      240

ccuaugcucg acgagggggu ggaaccggau gacguggauu gcugguguaa caccacaagc      300

accugggucg uguauggaac augccaccac aagaaaggcg aggcccggcg cuccagacgg      360

gcgguuaccc ugcccucuca uagcacgcgc aaacuucaaa ccagauccca gacauggcug      420

gaaagccggg aguacaccaa acaccucauu cgcguggaga auuggaucuu uagaaacccu      480

ggcuucgccc uggcugccgc ugccaucgcg uggcugcucg ggucuuccac aagccagaag      540

gucauuuauc uggugaugau ccuucugauu gcccccgcuu acucuauccg guguauugga      600

guuuccaauc gcgacuuugu ggaaggcaug agcgggggaa ccugggucga uguggugcuc      660

gagcauggcg ggugcgucac gguuauggcc caggacaagc cuacagugga uaucgaacug      720

gugaccacaa ccgucucuaa cauggcugag gugagauccu acuguuauga ggccagcauu      780

uccgacaugg ccucugauag ccggugcccc acccaaggag aggcuuaccu ugacaaacag      840

uccgacacac aguauguuug uaagcgcacg cugguggaua gaggcugggg caaugggugc      900

ggacucuucg gcaaagggag ccuggugacc ugcgcgaagu uugccuguuc uaaaaagaug      960

acaggaaagu ccauccagcc ugaaaaccug gaauaccgga ucaugcucag cguccacggc     1020

ucucaacaca gcgggaugau uguuaaugac accggacaug agacagauga aaacagagcu     1080

aaaguggaga ucacccccaa uuccccgcgc gccgaggcca cgcugggcgg cuucgggucu     1140

cuuggacugg acugcgaacc ucggacaggc cucgauuuua gcgaccugua uuaccuuacc     1200

augaacaaua agcacuggcu gguccauaaa gagugguucc acgauauucc ccucccuugg     1260

cacgcugggg ccgacaccgg aacaccccau uggaacaaua aggaagcgcu gguggaguuu     1320

aaagaugcuc acgccaagag acagaccguc gugguucugg gcucccagga aggggccgug     1380

cauacagcuc ucgccggcgc ucuggaggcc gagauggacg gagcgaaagg ccgccuuagc     1440

ucugggcacc ugaagugucg gcucaagaug gauaaacuga gacuuaaggg agucuccuau     1500

agccugugca cggccgcuuu caccuuuaca aaaaucccgg ccgaaacccu ccauggcaca     1560

gugaccguug aggugcagua cgcugggacg gacggaccuu guaagguccc cgcccaaaug     1620

gcgguggaca ugcagacccu gacaccugug ggccgccuga uuaccgccaa ccccguuauc     1680

acagaaucua ccgagaacuc caaaaugaug cucgaacugg aucccccuuu cggggacagc     1740

uacaucguca uuggcguggg agagaagaag aucacgcacc acuggcaucg guccggcucu     1800

acacuuggga aggcuuuuag caccacacug aagggagccc agagacucgc ugcccugggc     1860

gauaccgccu gggacuucgg guccauugga ggcguguuca auucuaucgg gcgcgcuguc     1920

caccaagugu uuggcggagc guuccggacc cuguuuggcg ggaugagcug gauuacacag     1980

ggacuuaugg gcgcccuccu gcuguggaug gggguuaacg cuagagaucg cuccaucgcc     2040

cucgccuucc uggcuacggg aggcgugcuu gucuuucugg ccaccaaugu gcaugcguga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10223
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10223
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccauc acccggcgcg gcagcgccua cuauauguac      120

cuggacagau ccgaugcugg gaaagccauu ucuuucgcua caacgcuugg aguuaacaag      180

ugucacgugc agaucaugga ccugggccau augugcgaug ccaccaugag cuaugagugu      240

cccaugcucg acgaaggggu ggagcccgau gacgucgauu gcugguguaa uacaaccucc      300

acaugggugg uuuacggaac cugccaccau aaaaagggcg aagcgcggcg cucuagacgg      360

gccgugacac ugccgagcca cuccacccgc aaacuucaaa cgagaagcca gaccuggcug      420

gagucucggg aauauacaaa gcaucucauu aaggucgaga acuggaucuu ucgcaauccc      480

ggcuucgcuc ugguggccgu ugcuaucgcc uggcugcucg gguccagcac cucucagaaa      540

gugauuuacc uggucaugau ccuucugauu gcgccugccu auuccaucag auguauugga      600

gugagcaacc gggacuuugu ggagggcaug ucugggggaa caugggucga uguugugcuc      660

gaacacggcg ggugcgugac cgucauggcu caggacaagc ccacggugga uaucgagcug      720

guuacaacca caguguccaa uauggccgaa guccgcagcu acuguuacga ggcuuccauu      780

ucugacaugg ccagcgauuc cagaugcccu acccaaggag aggccuaucu ugacaaacag      840

agcgacaccc aguacgugug uaagcggaca cugguugauc gcggcugggg caacgggugc      900

ggacucuucg gcaaaggguc ucuggugacg ugcgcuaagu uuaccuguuc caagaaaaug      960

acaggaaaga gcauccagcc cgaaaaucug gaguauagaa ucaugcucuc uguccacggc     1020

agccaacauu ccgggaugau uggauacgaa accgacgagg aucgggcgaa aguggaaguc     1080

acaccuaacu cuccgagagc cgaggcuacc cugggcggcu ucgggagccu uggacuggac     1140

ugcgagcccc gcacgggccu cgauuuuucc gaccuguauu accuuacaau gaauaacaag     1200

cacuggcugg ugcauaaaga augguuccac gauaucccuc uccccuggca cgccggggcc     1260

gacaccggaa ccccucauug gaauaacaag gaggcucugg uugaauuuaa agaugcccac     1320

gcgaagcggc agacaguggu cgugcugggc agccaggagg gggcuguuca uaccgcccuc     1380

gccggcgcuc uggaagccga gauggacgga gcuaagggca gacuuuucuc ugggcaccug     1440

aaaugucgcc ucaagaugga uaaacugcgg cuuaagggag uguccuauag ccugugcaca     1500

gccgcguuua cguucaccaa aguccccgcc gagacacucc auggcaccgu gacaguggaa     1560

guucaguacg cugggaccga cggaccgugu aagauuccug uccaaauggc cguggacaug     1620

cagacgcuga cccccguggg cagacugauc acagcuaauc cugucauuac cgagucuaca     1680

gaaaacucca agaugaugcu cgagcuggau ccucccuuug gggacagcua caucgugauc     1740

ggcguuggag auaaaaagau uacccaccac uggcaucgcu ccggcucuac gcuugggaaa     1800

gccuucagca caacccugaa gggagcgcag cggcucgccg cucugggcga cacagccugg     1860

gauuucgggu ccaucggagg cguguuuaau ucuauuggga gagcugucca ccaaguguuc     1920

ggcggagccu uucgcacccu guucggcggg augagcugga ucacccaggg acuuaugggc     1980

gcccuccugc uguggauggg gguuaacgcu cgggacagau ccauugcgcu cgccuuucug     2040

gcuacaggag gcgugcuugu cuuccuggcc acgaacgugc augccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10224
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10224
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccguu acccggcgcg gcagcgccua cuauauguac      120

cuggacagaa acgaugcugg ggaggccauc uccuucccca caacgcuugg aaugaauaaa      180

uguuauauuc agaucaugga ccugggccac augugcgaug cuaccauguc uuacgaaugu      240

ccuaugcucg acgagggggu ggaaccggau gacguggauu gcugguguaa cacaaccagc      300

acaugggucg uguauggaac cugccaucac aagaaaggcg aggcccggcg cuccagacgg      360

gcgguuacac ugcccucuca uagcacccgc aaacuucaaa cgagauccca gaccuggcug      420

gaaagccggg aguacacaaa acaccucauu cgcguggaga auuggaucuu uagaaacccu      480

ggcuucgccc uggcugccgc ugccaucgcg uggcugcucg ggucuuccac cagccagaag      540

gucauuuauc uggugaugau ccuucugauu gcccccgcuu acucuauccg guguauugga      600

guuuccaauc gcgacuuugu ggaaggcaug agcgggggaa caugggucga uguggugcuc      660

gagcauggcg ggugcgucac cguuauggcc caggacaagc cuacggugga uaucgaacug      720

gugacaacca cagucucuaa cauggcugag gugagauccu acuguuauga ggccagcauu      780

uccgacaugg ccucugauag ccggugcccc acccaaggag aggcuuaccu ugacaaacag      840

uccgacaccc aguauguuug uaagcgcaca cugguggaua gaggcugggg caaugggugc      900

ggacucuucg gcaaagggag ccuggucacg ugcgcgaagu uugccuguuc uaaaaagaug      960

accggaaagu ccauccagcc ugaaaaccug gaauaccgga ucaugcucag cgugcacggc     1020

ucucaacaca gcgggaugau uguuaaugac acaggacaug agaccgauga aaacagagcu     1080

aaaguggaga ucacacccaa uuccccgcgc gccgaggcca cccugggcgg cuucgggucu     1140

cuuggacugg acugcgaacc ucggacgggc cucgauuuua gcgaccugua uuaccuuaca     1200

augaacaaua agcacuggcu gguccauaaa gagugguucc acgauauucc ccucccuugg     1260

cacgcugggg ccgacaccgg aacaccccau uggaacaaua aggaagcgcu gguggaguuu     1320

aaagaugcuc acgccaagag acagaccguc gugguucugg gcucccagga aggggccgug     1380

cauaccgcuc ucgccggcgc ucuggaggcc gagauggacg gagcgaaagg ccgccuuagc     1440

ucugggcacc ugaagugucg gcucaagaug gauaaacuga gacuuaaggg agucuccuau     1500

agccugugca cagccgcuuu cacguuuacc aaaaucccgg ccgaaacacu ccauggcacc     1560

gugacaguug aggugcagua cgcugggacc gacggaccuu guaagguccc cgcccaaaug     1620

gcgguggaca ugcagacgcu gaccccugug ggccgccuga uuacagccaa ccccguuauc     1680

accgaaucua cagagaacuc caaaaugaug cucgaacugg aucccccuuu cggggacagc     1740

uacaucguca uuggcguggg agagaagaag aucacccacc acuggcaucg guccggcucu     1800

acgcuuggga aggcuuuuag cacaacccug aagggagccc agagacucgc ugcccugggc     1860

gauacagccu gggacuucgg guccauugga ggcguguuca auucuaucgg gcgcgcuguc     1920

caccaagugu uuggcggagc guuccggacc cuguuuggcg ggaugagcug gauuacccag     1980

ggacuuaugg gcgcccuccu gcuguggaug gggguuaacg cuagagaucg cuccaucgcc     2040

cucgccuucc uggcuacagg aggcgugcuu gucuuucugg ccacgaaugu gcaugcguga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10225
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10225
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccguua cccuucccuc ccacucuaca agaaagcugc agacccggag ccaaacaugg      120

cucgaguccc gcgaauacac caaacaucug auuagagugg agaacuggau cuuccggaau      180

ccuggauuug cgcuugcugc cgccgcuauu gccuggcugc ucggcucuag cacaucccag      240

aaggugaucu aucuggucau gauucugcuc aucgcuccgg ccuacucuau ccgcugcauu      300

ggggugagca acagagacuu cguugaagga auguccggcg ggaccugggu ggauaucguc      360

cuggagcacg gaggcugugu gacgguuaug gcgcaggaca aacccaccgu ggauauugaa      420

cuugucacaa ccacagugag caauauggcc gaggugcggu cuuauugcua cgaggcuucc      480

aucagcgaca uggccucuga uucccgcugu ccuacccagg gcgaggcuua ucuggacaag      540

caaagcgaua cgcaguacgu cugcaaaaga acacucguug accgggggug gggaaacggc      600

ugugggcugu uuggaaaggg cucucuugug accugcgcca aguucgcgug uuccaaaaag      660

augacaggga aaagcauuca gcccgaaaau cuggaauauc gcaucaugcu cuccgugcau      720

ggaucucagc acagcggcau gauugucaac gauaccggcc augagaccga cgagaauaga      780

gccaaggugg aaaucacacc uaacagcccc cgggcugagg ccacgcuugg gggauuuggc      840

ucccuggggc ucgauugcga accucgcacc ggacuggacu ucucugaucu uuacuaccug      900

acaaugaaua acaaacacug gcucguucac aaggaguggu uucaugacau cccgcugccc      960

uggcacgcug gcgccgacac cgggacaccu cauuggaaua acaaggaagc ccuuguggag     1020

uucaaagaug cucacgcgaa gagacaaacc gucgugguuc ugggauccca ggagggcgcc     1080

gugcacacgg cucucgccgg cgcccuggaa gcugagaugg acggggccaa aggacggcug     1140

agcucuggcc aucucaagug ucgccugaaa auggauaagc ugagacugaa aggggucagc     1200

uauucccucu gcacagcggc uuuuaccuuc accaagauuc ccgccgaaac acugcacgga     1260

accgugacag ucgaggugca guacgccggc acggacgggc cuuguaaggu ucccgcucag     1320

auggccgugg auaugcaaac ccuuacaccg gucggccggc ugaucaccgc uaauccugug     1380

auuacagaau cuaccgagaa cagcaaaaug augcucgagc uggauccccc uuuuggagac     1440

uccuauaucg uuauuggcgu gggggaaaag aaaaucacgc aucacuggca uagaagcgga     1500

ucuaccaucg gcaaggccuu cgaggcgaca guccgcgggg ccaaacggau ggcugugcug     1560

ggagacaccg ccugggauuu uggcuccguu gggggcgcuc ucaauagccu gggaaagggc     1620

auucaccaga ucuucggggc cgcguucaag ucucuuuuug gaggcauguc cugguucagc     1680

cagauucuga ucgggacacu ccugaugugg cugggacuua acaccaaaaa cggcuccauu     1740

ucucucaugu gccuggcccu cgggggcgug cugaucuuuc ugagcacggc ugucuccgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10226
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10226
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccguua cccuucccuc ccacucuaca agaaagcugc agacccggag ccaaacaugg      120

cucgaguccc gcgaauacac caaacaucug auuagagugg agaacuggau cuuccggaau      180

ccuggauuug cgcuugcugc cgccgcuauu gccuggcugc ucggcucuag cacaucccag      240

aaggugaucu aucuggucau gauucugcuc aucgcuccgg ccuacucuau ccgcugcauu      300

ggggugagca acagagacuu cguugaagga auguccggcg ggaccugggu ggaugucgug      360

cuggagcacg gaggcugugu uacggugaug gcgcaggaca aacccaccgu cgauaucgaa      420

cuugugacaa ccacagugag caauauggcc gagguccggu cuuauugcua cgaggcuucc      480

auuagcgaca uggccucuga uucccgcugu ccuacccagg gcgaggcuua ucuggacaag      540

caaagcgaua cgcaguacgu uugcaaaaga acacucgugg accgggggug gggaaacggc      600

ugugggcugu uuggaaaggg cucucuugug accugcgcca aguucgcgug uuccaaaaag      660

augacaggga aaagcaucca gcccgaaaau cuggaauauc gcauuaugcu cuccguccau      720

ggaucucagc acagcggcau gaucgugaac gauaccggcc augagaccga cgagaauaga      780

gccaagguug aaauuacacc uaacagcccc cgggcugagg ccacgcuugg gggauuuggc      840

ucccuggggc ucgauugcga accucgcacc ggacuggacu ucucugaucu uuacuaccug      900

acaaugaaua acaaacacug gcucgugcac aaggaguggu uucaugacau cccgcugccc      960

uggcacgcug gcgccgacac cgggacaccu cauuggaaua acaaggaagc ccuugucgag     1020

uucaaagaug cucacgcgaa gagacaaacc gugguugugc ugggauccca ggagggcgcc     1080

guccacacgg cucucgccgg cgcccuggaa gcugagaugg acggggccaa aggacggcug     1140

agcucuggcc aucucaagug ucgccugaaa auggauaagc ugagacugaa aggggugagc     1200

uauucccucu gcacagcggc uuuuaccuuc accaagaucc ccgccgaaac acugcacgga     1260

accgucacag uggagguuca guacgccggc acggacgggc cuuguaaggu gcccgcucag     1320

auggccgucg auaugcaaac ccuuacaccg gugggccggc ugauuaccgc uaauccuguu     1380

aucacagaau cuaccgagaa cagcaaaaug augcucgagc uggauccccc uuuuggagac     1440

uccuauauug ugaucggcgu cggggaaaag aaaauuacgc aucacuggca uagaagcgga     1500

ucuaccaucg gcaaggccuu cgaggcgaca gugcgcgggg ccaaacggau ggcugugcug     1560

ggagacaccg ccugggauuu uggcuccguu gggggcgcuc ucaauagccu gggaaagggc     1620

auccaccaga uuuucggggc cgcguucaag ucucuuuuug gaggcauguc cugguucagc     1680

cagauccuga uugggacacu ccugaugugg cugggacuua acgccaaaaa cggcuccauc     1740

ucucucaugu gccuggcucu cgggggcguc cugauuuuuc ugagcaccgc cguguccgcu     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10227
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10227
gggagaaagc uuaccaugac cagcaucggc auuaucgggc ugcugcugac aacggccaug       60

gcugccguga cccuucccuc ccacucuaca agaaagcugc agacccggag ccaaacaugg      120

cucgaguccc gcgaauacac caaacaucug auuaaggucg agaacuggau cuucagaaau      180

ccuggauuug cgcuuguugc uguggccauu gccuggcugc ucggcucuag cacaucccag      240

aaagugaucu aucuggucau gauccugcuc auugcuccgg ccuacucuau ccggugcauu      300

ggggugagca accgcgacuu cguugaagga auguccggcg ggaccugggu ggaugucgug      360

cuggagcacg gaggcugugu uacggugaug gcucaggaca agcccaccgu cgauaucgaa      420

cuugugacaa ccacagugag caauauggcc gaggucagau cuuauugcua cgaggcgucc      480

auuagcgaca uggccucuga uucccggugu ccuacccagg gcgaggcuua ucuggacaaa      540

caaagcgaua cgcaguacgu uugcaagcgc acacucgugg acagagggug gggaaacggc      600

ugugggcugu uuggaaaggg cucucuugug accugcgcca aauucacaug uuccaagaaa      660

augaccggaa agagcaucca gcccgaaaau cuggaauauc ggauuaugcu cuccguccau      720

gggucucagc acagcggcau gaucggcuac gagaccgaug aggaccgcgc uaaaguggaa      780

guuacaccua acucccccag agccgaggcg acgcucgggg gauuuggcag ccuggggcug      840

gauugcgaac cucggaccgg acuggacuuc ucugaucuuu acuaucugac aaugaauaac      900

aagcauuggc ucgugcacaa ggagugguuu cacgacaucc cgcugcccug gcaugccggc      960

gcugacaccg ggacaccuca cuggaauaac aaagaagccc uugucgaguu caaggaugcu     1020

caugccaaac gccaaaccgu gguugugcug ggaucccagg agggcgccgu ccacacggcu     1080

cucgcgggcg cccuggaagc ugagauggac ggggccaagg gaagacuguu uagcggccac     1140

cucaaauguc ggcugaagau ggauaaacuu cgccugaagg gggugucuua cagccucugc     1200

acagccgcuu ucaccuuuac caaggucccc gccgaaacac ugcauggaac cgugacaguu     1260

gaggugcagu augcgggcac ggacgggccu uguaaaauuc ccguccagau ggcuguggau     1320

augcaaaccc uuacaccggu uggcagacug aucaccgcca auccugugau uacagaaucc     1380

accgagaacu cuaagaugau gcucgagcug gaucccccuu ucggagacag cuacaucguc     1440

auuggcgugg gggacaaaaa gaucacgcac cauuggcacc gguccggaag caccaucggc     1500

aaagccuuug aagcuacagu gagaggggcc aagcgcaugg cuguucuggg agauaccgcc     1560

ugggacuucg gcucugucgg gggcguguuc aauucccucg gaaagggcau ucaucagauc     1620

uuuggggcgg ccuucaaaag ccuguuugga ggcaugucuu gguucuccca gauucuuauc     1680

gggacacugc ucguguggcu gggacugaac accaagaaug gcagcauuuc ccuuacgugc     1740

cucgcucucg ggggcgucau gaucuuucug ucuacagccg ugagcgcuug aggacuaguu     1800

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     1860

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  10228
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10228
gggagaaagc uuaccaugac cagcgugggc aucgucgggc ugcuccugac aacggccaug       60

gcugccguua cccuucccuc ccacucuaca agaaagcugc agacccggag ccaaacaugg      120

cucgaguccc gcgaauacac caaacaucug auuagagugg agaacuggau cuuccggaau      180

ccuggauuug cgcuugcugc cgccgcuauu gccuggcugc ucggcucuag cacaucccag      240

aaggugaucu aucuggucau gauucugcuc aucgcuccgg ccuacucuau ccgcugcauu      300

ggggugagca acagagacuu cguugaagga auguccggcg ggaccugggu ggaugucgug      360

cuggagcacg gaggcugugu uacggugaug gcgcaggaca aacccaccgu cgauaucgaa      420

cuugugacaa ccacagugag caauauggcc gagguccggu cuuauugcua cgaggcuucc      480

auuagcgaca uggccucuga uucccgcugu ccuacccagg gcgaggcuua ucuggacaag      540

caaagcgaua cgcaguacgu uugcaaaaga acacucgugg accgggggug gggaaacggc      600

ugugggcugu uuggaaaggg cucucuugug accugcgcca aguucgcgug uuccaaaaag      660

augacaggga aaagcaucca gcccgaaaau cuggaauauc gcauuaugcu cuccguccau      720

ggaucucagc acagcggcau gaucgugaac gauaccggcc augagaccga cgagaauaga      780

gccaagguug aaauuacacc uaacagcccc cgggcugagg ccacgcuugg gggauuuggc      840

ucccuggggc ucgauugcga accucgcacc ggacuggacu ucucugaucu uuacuaccug      900

acaaugaaua acaaacacug gcucgugcac aaggaguggu uucaugacau cccgcugccc      960

uggcacgcug gcgccgacac cgggacaccu cauuggaaua acaaggaagc ccuugucgag     1020

uucaaagaug cucacgcgaa gagacaaacc gugguugugc ugggauccca ggagggcgcc     1080

guccacacgg cucucgccgg cgcccuggaa gcugagaugg acggggccaa aggacggcug     1140

agcucuggcc aucucaagug ucgccugaaa auggauaagc ugagacugaa aggggugagc     1200

uauucccucu gcacagcggc uuuuaccuuc accaagaucc ccgccgaaac acugcacgga     1260

accgucacag uggagguuca guacgccggc acggacgggc cuuguaaggu gcccgcucag     1320

auggccgucg auaugcaaac ccuuacaccg gugggccggc ugauuaccgc uaauccuguu     1380

aucacagaau cuaccgagaa cagcaaaaug augcucgagc uggauccccc uuuuggagac     1440

uccuauauug ugaucggcgu cggggaaaag aaaauuacgc aucacuggca uagaagcgga     1500

ucuaccaucg gcaaggccuu cgaggcgaca gugcgcgggg ccaaacggau ggcugugcug     1560

ggagacaccg ccugggauuu uggcuccguu gggggcgcuc ucaauagccu gggaaagggc     1620

auccaccaga uuuucggggc cgcguucaag ucucuuuuug gaggcauguc cugguucagc     1680

cagauccuga uugggacacu ccugaugugg cugggacuua acaccaaaaa cggcuccauc     1740

ucucucaugu gccuggcccu cgggggcguc cugauuuuuc ugagcacggc uguguccgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10229
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10229
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gcugugaccc ugccuagcca cuccacaaga      120

aagcugcaaa cgcggucuca gaccuggcug gaaagccgcg aguacacaaa acaucucauu      180

agaguggaaa acuggaucuu ccggaauccg ggauuugccc uggccgcugc cgcuauugcc      240

uggcuucugg gcuccucuac cagccagaag gucaucuauc ucgugaugau ccugcugauu      300

gcgcccgccu acuccauccg cugcauuggg guuagcaaca gagacuucgu ggagggaaug      360

ucuggcggga caugggucga uaucgugcuc gaacacggag gcuguguuac cgugauggcu      420

caggacaaac cuacagucga uauugagcug gugaccacga ccguguccaa uauggccgag      480

guccggagcu auugcuacga ggcuucuauc uccgacaugg ccagcgauuc ucgcuguccc      540

acacaaggcg aagcguaucu ugacaagcag uccgauaccc aguacguuug caaaagaaca      600

cugguggacc gggggugggg aaacggcugu gggcucuuug gaaagggcag ccuggugacc      660

ugcgccaagu ucgcuuguuc caaaaagaug acggggaaau cuauucagcc ugaaaaucuu      720

gaguaucgca ucaugcugag cguccaugga ucccaacaca gcggcaugau cgugaacgau      780

acaggccaug agaccgacga aaauagagcc aagguugaga uuacacccaa cucuccucgg      840

gcugaagcca cccucggggg auuuggcucc cuggggcugg auugcgagcc gcgcaccgga      900

cucgacuuca gcgaucugua cuaccuuaca augaauaaca aacacuggcu ggugcacaag      960

gaaugguuuc augacauccc ccucccuugg cacgccggcg cugacacggg gacaccccau     1020

uggaauaaca aggaggcgcu ggucgaguuc aaagaugccc acgcuaagag acagacagug     1080

guugugcuug gaucucagga aggcgccguc cacaccgccc uggcuggcgc ccucgaggcg     1140

gaaauggacg gggcuaaagg acggcugagc uccggccauc ugaagugucg ccucaaaaug     1200

gauaagcuga gacuuaaagg ggugucuuau agccugugca cagccgccuu uaccuucacg     1260

aagauuccug cugagacacu ccacggaacc gucaccgugg aaguucagua cgccggcaca     1320

gacgggcccu guaaggugcc ggcucaaaug gccgucgaua ugcagacccu gacaccugug     1380

ggccggcuua ucacggcgaa ucccguuauu accgagucca cagagaacag caaaaugaug     1440

cuggaacucg acccucccuu uggagauucu uauaucguga ucggcgucgg ggagaagaaa     1500

auuacccauc acuggcauag auccggaagc acaaucggca aggccuucga agcuaccgug     1560

cgcggggcca aacggauggc ugugcuggga gacacggccu gggauuucgg cucuguuggg     1620

ggcgcgcuua auucccucgg aaagggcauu caccagaucu uuggggccgc uuucaagagc     1680

cuguuuggag gcauguccug guucucucaa auucuuaucg ggacccugcu cauguggcug     1740

ggacugaaca ccaaaaacgg cagcaucucc cuuaugugcc ucgcccucgg gggcguccug     1800

auuuuucugu cuaccgcugu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10230
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10230
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gcugugaccc ugccuagcca cuccacaaga      120

aagcugcaaa cgcggucuca gaccuggcug gaaagccgcg aguacacaaa acaucucauu      180

agaguggaaa acuggaucuu ccggaauccg ggauuugccc uggccgcugc cgcuauugcc      240

uggcuucugg gcuccucuac cagccagaag gucaucuauc ucgugaugau ccugcugauu      300

gcgcccgccu acuccauccg cugcauuggg guuagcaaca gagacuucgu ggagggaaug      360

ucuggcggga caugggucga ugugguucuc gaacacggag gcugugugac cgucauggcu      420

caggacaaac cuacagugga uaucgagcug gugaccacga ccgucuccaa uauggccgag      480

guucggagcu auugcuacga ggcuucuauu uccgacaugg ccagcgauuc ucgcuguccc      540

acacaaggcg aagcguaucu ugacaagcag uccgauaccc aguacgugug caaaagaaca      600

cugguggacc gggggugggg aaacggcugu gggcucuuug gaaagggcag ccuggugacc      660

ugcgccaagu ucgcuuguuc caaaaagaug acggggaaau cuauccagcc ugaaaaucuu      720

gaguaucgca uuaugcugag cguccaugga ucccaacaca gcggcaugau cguuaacgau      780

acaggccaug agaccgacga aaauagagcc aagguggaga ucacacccaa cucuccucgg      840

gcugaagcca cccucggggg auuuggcucc cuggggcugg auugcgagcc gcgcaccgga      900

cucgacuuca gcgaucugua cuaccuuaca augaauaaca aacacuggcu gguccacaag      960

gaaugguuuc augacauucc ccucccuugg cacgccggcg cugacacggg gacaccccau     1020

uggaauaaca aggaggcgcu gguggaguuc aaagaugccc acgcuaagag acagaccguu     1080

gugguccuug gaucucagga aggcgccgug cacaccgccc uggcuggcgc ccucgaggcg     1140

gaaauggacg gggcuaaagg acggcugagc uccggccauc ugaagugucg ccucaaaaug     1200

gauaagcuga gacuuaaagg ggucucuuau agccugugca cagccgccuu uaccuucacg     1260

aagaucccug cugagacacu ccacggaacc gugaccguug aagugcagua cgccggcaca     1320

gacgggcccu guaagguccc ggcucaaaug gccguggaua ugcagacccu gacaccuguu     1380

ggccggcuua uuacggcgaa ucccgugauc accgagucca cagagaacag caaaaugaug     1440

cuggaacucg acccucccuu uggagauucu uauauuguca ucggcguggg ggagaagaaa     1500

aucacccauc acuggcauag auccggaagc acaauuggca aggccuucga agcuaccgug     1560

cgcggggcca aacggauggc uguucuggga gacacggccu gggauuucgg cucugucggg     1620

ggcgcgcuua auucccucgg aaagggcauc caccagauuu uuggggccgc uuucaagagc     1680

cuguuuggag gcauguccug guucucucaa auccuuauug ggacccugcu cauguggcug     1740

ggacugaacg ccaaaaacgg cagcaucucc cuuaugugcc ucgcucucgg gggcgugcug     1800

aucuuucugu cuacagccgu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10231
<211>  2020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10231
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gcugugaccc ugccuagcca cuccacaaga      120

aaacuucaaa cgcggucuca gaccuggcug gaaagccgcg aguacacaaa gcaucucauu      180

aagguggaaa acuggaucuu cagaaauccg ggauuugccc uggucgccgu ggcuauugcc      240

uggcuucugg gcuccucuac cagccagaaa guuaucuauc ucgugaugau ccugcugauu      300

gcucccgccu acuccauccg gugcauuggg gucagcaacc gcgacuucgu ggagggaaug      360

ucuggcggga cauggguuga ugugguccuc gaacacggag gcugugugac cgugauggcg      420

caggacaagc cuacagucga uaucgagcug guuaccacga ccguguccaa uauggccgag      480

gugagaagcu auugcuacga agccucuauu uccgacaugg cuagcgauuc ucgguguccc      540

acacaaggcg aggcuuaucu ugacaaacag uccgauaccc aguacgucug caagcgcaca      600

cugguggaca gagggugggg aaacggcugu gggcucuuug gaaagggcag ccugguuacc      660

ugcgccaaau ucacguguuc caagaaaaug acagggaagu cuauccagcc ugaaaaucuu      720

gaguaucgga uuaugcugag cgugcaugga ucccaacaca gcggcaugau cggcuacgag      780

accgaugaag accgcgcgaa agucgaggug acacccaacu cuccuagagc cgaagcuacc      840

cucgggggau uuggcucccu ggggcuggau ugcgagccgc ggaccggacu cgacuucagc      900

gaucuguacu aucuuacaau gaauaacaag cauuggcugg uucacaagga augguuucac      960

gacauccccc ucccuuggca ugccggcgcu gacacgggga caccccacug gaauaacaaa     1020

gaggcccugg uggaguucaa ggaugcccau gcuaaacgcc agaccgucgu gguccuugga     1080

ucucaggaag gcgcggugca caccgcccug gcuggcgccc ucgaggccga aauggacggg     1140

gcuaagggaa gacuguuuag cggccaccug aaaugucggc ucaagaugga uaaacugcgc     1200

cuuaaggggg uuuccuacuc ucugugcaca gccgcguuca ccuuuacgaa ggugccugcu     1260

gagacacucc auggaaccgu caccguggaa guucaguaug ccggcacaga cgggcccugu     1320

aaaauuccgg ugcaaauggc cgucgauaug cagacccuga caccuguggg cagacuuauc     1380

acggcuaauc ccgugauuac cgagagcaca gagaacucca agaugaugcu ggaacucgac     1440

ccucccuucg gagauagcua caucguuauu ggcgucgggg acaaaaagau cacccaccau     1500

uggcaccggu cuggauccac aaucggcaaa gccuucgagg cuaccgugag aggggccaag     1560

cgcauggcgg ugcugggaga uacggccugg gacuuuggca gcgucggggg cguguucaau     1620

ucucugggaa agggcauuca ucagaucuuu ggggcugccu ucaaaucccu cuuuggaggc     1680

augagcuggu ucucccaaau ucugaucggg acccuucugg uuuggcucgg acugaacaca     1740

aagaauggcu cuauuagccu gaccugccuu gcucucgggg gcgugaugau cuuucugucc     1800

acagccgucu cugcgugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     1860

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1980

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2020


<210>  10232
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10232
gggagaaagc uuaccauggc caucagcggc gugcccgucc ugggguucuu uauuaucgcu       60

guucucaugu ccgcccagga gucuugggcg gcugugaccc ugccuagcca cuccacaaga      120

aagcugcaaa cgcggucuca gaccuggcug gaaagccgcg aguacacaaa acaucucauu      180

agaguggaaa acuggaucuu ccggaauccg ggauuugccc uggccgcugc cgcuauugcc      240

uggcuucugg gcuccucuac cagccagaag gucaucuauc ucgugaugau ccugcugauu      300

gcgcccgccu acuccauccg cugcauuggg guuagcaaca gagacuucgu ggagggaaug      360

ucuggcggga caugggucga ugugguucuc gaacacggag gcugugugac cgucauggcu      420

caggacaaac cuacagugga uaucgagcug gugaccacga ccgucuccaa uauggccgag      480

guucggagcu auugcuacga ggcuucuauu uccgacaugg ccagcgauuc ucgcuguccc      540

acacaaggcg aagcguaucu ugacaagcag uccgauaccc aguacgugug caaaagaaca      600

cugguggacc gggggugggg aaacggcugu gggcucuuug gaaagggcag ccuggugacc      660

ugcgccaagu ucgcuuguuc caaaaagaug acggggaaau cuauccagcc ugaaaaucuu      720

gaguaucgca uuaugcugag cguccaugga ucccaacaca gcggcaugau cguuaacgau      780

acaggccaug agaccgacga aaauagagcc aagguggaga ucacacccaa cucuccucgg      840

gcugaagcca cccucggggg auuuggcucc cuggggcugg auugcgagcc gcgcaccgga      900

cucgacuuca gcgaucugua cuaccuuaca augaauaaca aacacuggcu gguccacaag      960

gaaugguuuc augacauucc ccucccuugg cacgccggcg cugacacggg gacaccccau     1020

uggaauaaca aggaggcgcu gguggaguuc aaagaugccc acgcuaagag acagacaguu     1080

gugguccuug gaucucagga aggcgccgug cacaccgccc uggcuggcgc ccucgaggcg     1140

gaaauggacg gggcuaaagg acggcugagc uccggccauc ugaagugucg ccucaaaaug     1200

gauaagcuga gacuuaaagg ggucucuuau agccugugca cagccgccuu uaccuucacg     1260

aagaucccug cugagacacu ccacggaacc gugaccguug aagugcagua cgccggcaca     1320

gacgggcccu guaagguccc ggcucaaaug gccguggaua ugcagacccu gacaccuguu     1380

ggccggcuua uuacggcgaa ucccgugauc accgagucca cagagaacag caaaaugaug     1440

cuggaacucg acccucccuu uggagauucu uauauuguca ucggcguggg ggagaagaaa     1500

aucacccauc acuggcauag auccggaagc acaauuggca aggccuucga agcuaccgug     1560

cgcggggcca aacggauggc uguucuggga gacacggccu gggauuucgg cucugucggg     1620

ggcgcgcuua auucccucgg aaagggcauc caccagauuu uuggggccgc uuucaagagc     1680

cuguuuggag gcauguccug guucucucaa auccuuauug ggacccugcu cauguggcug     1740

ggacugaaca ccaaaaacgg cagcaucucc cuuaugugcc ucgcccucgg gggcgugcug     1800

aucuuucugu cuaccgcugu gagcgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10233
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10233
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg cgguuacgcu gcccucccau ucuacccgga aacuucagac acgcagccaa      120

accuggcugg aguccagaga auacacaaaa caccucauuc ggguggagaa cuggaucuuu      180

cgcaauccug gcuucgcucu ggccgccgcu gccauugcuu ggcuucuggg gucuagcacc      240

ucccagaagg ugaucuaucu cgucaugauu cugcugaucg ccccggcgua cucuaucaga      300

ugcauuggag ugagcaaccg ggauuuuguu gaaggcaugu ccgggggaac auggguggac      360

aucguccucg agcauggcgg gugugugacc guuauggccc aggauaaacc cacgguggac      420

auugaacugg ucaccacaac cgugagcaau auggcugagg ugcgcucuua uugcuacgag      480

gccuccauca gcgauauggc uucugacucc agauguccua cacagggaga agccuaucuu      540

gauaagcaaa gcgacaccca guacgucugc aaacggacgc ugguugaucg cggcuggggc      600

aacgggugug gacucuucgg caaggggucu cuggugacau gcgcgaaguu ugccuguucc      660

aaaaagauga ccggaaaaag cauucagccc gagaaucuug aauauagaau caugcugucc      720

gugcacggcu cucagcauag cgggaugauu gucaacgaca caggacacga gaccgaugag      780

aaucgggcua agguggaaau caccccuaac agcccccgcg ccgaggcuac acucggcggc      840

uucggguccc ugggacugga cugcgaaccu agaacgggcc ucgauuuuuc ugaccuguac      900

uaccuuacca ugaauaacaa acacuggcug guucauaagg agugguucca cgacaucccg      960

cuccccuggc augccggggc cgauacagga accccucacu ggaauaacaa ggaagcucug     1020

guggaguuua aagacgcgca cgccaagcgg caaacagucg ugguucuugg cucccaggag     1080

ggggcugugc auaccgcccu ggccggagcu cucgaagccg agauggaugg cgcgaaaggc     1140

cgccugagcu cugggcaccu gaaguguaga cucaaaaugg acaagcugcg gcuuaaagga     1200

gucagcuauu cccugugcac ggcugccuuc acauuuacca agauucccgc cgaaacccuc     1260

cauggcacag ugaccgucga ggugcaguac gcugggacag auggaccuug uaagguuccc     1320

gcccagaugg cuguggacau gcaaacgcug accccggucg gcagacuuau cacagccaau     1380

ccugugauua ccgaaucuac agagaacagc aaaaugaugc uggagcucga ucccccuuuc     1440

ggggacuccu auaucguuau uggcguggga gaaaagaaaa ucacccacca uuggcaccgc     1500

agcggcucua cgaucgggaa ggcguuugag gccaccgucc ggggagcuaa aagaauggcc     1560

gugcugggcg auacagcuug ggacuucggg uccgugggag gcgcccugaa uagccucggg     1620

aagggcauuc accagaucuu cggagcggcc uuuaagucuc uguucggcgg gauguccugg     1680

uuuagccaga uucuuaucgg aacccugcuc auguggcugg gccugaacac aaagaacggg     1740

uccauuucuc uuaugugccu cgcucuggga ggcguucuga ucuuccucag caccgccguc     1800

uccgcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10234
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10234
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg cgguuacgcu gcccucccau ucuacccgga aacuucagac acgcagccaa      120

accuggcugg aguccagaga auacacaaag caccucauuc ggguggagaa cuggaucuuu      180

cgcaauccug gcuucgcucu ggccgccgcu gccauugcuu ggcuucuggg gucuagcacc      240

ucccagaagg ugaucuaucu cgucaugauu cugcugaucg ccccggcgua cucuaucaga      300

ugcauuggag ugagcaaccg ggauuuuguu gaaggcaugu ccgggggaac auggguggac      360

gucgugcucg agcauggcgg guguguuacc gugauggccc aggauaaacc cacggucgac      420

aucgaacugg ugaccacaac cgugagcaau auggcugagg uccgcucuua uugcuacgag      480

gccuccauua gcgauauggc uucugacucc agauguccua cacagggaga agccuaucuu      540

gauaagcaaa gcgacaccca guacguuugc aaacggacgc ugguggaucg cggcuggggc      600

aacgggugug gacucuucgg caaggggucu cuggugacau gcgcgaaguu ugccuguucc      660

aaaaagauga ccggaaaaag cauccagccc gagaaucuug aauauagaau uaugcugucc      720

guccacggcu cucagcauag cgggaugauc gugaacgaca caggacacga gaccgaugag      780

aaucgggcua agguugaaau uaccccuaac agcccccgcg ccgaggcuac acucggcggc      840

uucggguccc ugggacugga cugcgaaccu agaacgggcc ucgauuuuuc ugaccuguac      900

uaccuuacca ugaauaacaa acacuggcug gugcauaagg agugguucca cgacaucccg      960

cuccccuggc augccggggc cgauacagga accccucacu ggaauaacaa ggaagcucug     1020

gucgaguuua aagacgcgca cgccaagcgg caaacagugg uugugcuugg cucccaggag     1080

ggggcugucc auaccgcccu ggccggagcu cucgaagccg agauggaugg cgcgaaaggc     1140

cgccugagcu cugggcaccu gaaguguaga cucaaaaugg acaagcugcg gcuuaaagga     1200

gugagcuauu cccugugcac ggcugccuuc acauuuacca agauccccgc cgaaacccuc     1260

cauggcacag ucaccgugga gguucaguac gcugggacag auggaccuug uaaggugccc     1320

gcccagaugg cugucgacau gcaaacgcug accccggugg gcagacuuau uacagccaau     1380

ccuguuauca ccgaaucuac agagaacagc aaaaugaugc uggagcucga ucccccuuuc     1440

ggggacuccu auauugugau cggcgucgga gaaaagaaaa uuacccacca uuggcaccgc     1500

agcggcucua cgaucgggaa ggcguuugag gccaccgugc ggggagcuaa aagaauggcc     1560

gugcugggcg auacagcuug ggacuucggg uccguuggag gcgcccugaa uagccucggg     1620

aagggcaucc accagauuuu cggagcggcc uuuaagucuc uguucggcgg gauguccugg     1680

uuuagccaga uccuuauugg aacccugcuc auguggcugg gccugaacgc uaaaaacggg     1740

uccaucucuc uuaugugccu cgcccuggga ggcguccuga uuuuccucag cacagcugug     1800

uccgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10235
<211>  1999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10235
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg cgguuacgcu gcccucccau ucuacccgga aacuucagac acgcagccaa      120

accuggcugg aguccagaga auacacaaaa caccucauua agguggagaa cuggaucuuu      180

cggaauccug gcuucgcucu gguggccguc gccauugcuu ggcuucuggg gucuagcacc      240

ucccagaaag ugaucuaucu cguuaugauu cugcugaucg ccccggcuua cucuauccgc      300

ugcauuggag ugagcaacag agauuuuguc gaaggcaugu ccgggggaac auggguggac      360

guugugcucg agcauggcgg gugugucacc gugauggccc aggauaagcc cacgguggac      420

aucgaacugg ucaccacaac cguuagcaau auggcggagg ugcggucuua uugcuacgag      480

gccuccauua gcgauauggc uucugacucc cgcuguccua cacagggaga agccuaucuu      540

gauaaacaaa gcgacaccca guacgugugc aagagaacgc uggucgaucg gggcuggggc      600

aacgggugug gacucuucgg caaggggucu cuggugacau gcgcuaaauu uaccuguucc      660

aagaaaauga caggaaagag cauccagccc gagaaucuug aauaucgcau uaugcugucc      720

guucacggcu cucagcauag cgggaugauc ggauacgaga ccgacgagga uagagccaaa      780

guggaaguca ccccuaacag cccccgggcg gaggccacac ucggcggcuu cgggucccug      840

ggacuggacu gcgaaccucg cacgggccuc gauuuuucug accuguacua ucuuaccaug      900

aauaacaagc acuggcuggu gcacaaggag ugguuccaug acauuccgcu ccccuggcac      960

gcuggggccg auacaggaac cccucauugg aauaacaaag aagcucuggu ugaguuuaag     1020

gacgcccacg ccaaaagaca aacagugguc gugcuuggcu cccaggaggg ggcuguccac     1080

accgcgcugg ccggagcucu cgaagccgag auggauggcg ccaagggccg gcuguucagc     1140

gggcaucuga aaugucgccu caagauggac aaacugagac uuaagggagu gucuuacagc     1200

cugugcacgg cugccuuuac auucaccaag guucccgcgg aaacccucca cggcacagug     1260

accgucgagg ugcaguaugc ugggacagau ggaccuugua aaauccccgu ucagauggcc     1320

guggacaugc aaacgcugac cccggucggc cggcuuauca cagccaaucc ugugauuacc     1380

gaauccacag agaacucuaa gaugaugcug gagcucgauc ccccuuuugg ggacagcuac     1440

aucgugauug gcguuggaga uaaaaagauc acccaucacu ggcauagauc cggcagcacg     1500

auugggaagg cuuucgaagc caccguccgc ggagcuaagc ggauggccgu gcugggcgac     1560

acagcguggg auuucggguc ugugggaggc gucuuuaauu cccuggggaa gggcauccac     1620

cagaucuucg gagccgcuuu uaaaagccuc uucggcggga ugucuugguu uucccagauu     1680

cugaucggaa cccuucuggu guggcucggc cugaacacaa agaaugggag cauuucccug     1740

accugccuug cccucggagg cguuaugauc uuccugucua cggcugugag cgccugagga     1800

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     1860

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     1980

uuucagagcc accagaauu                                                  1999


<210>  10236
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10236
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc ugccacaaga       60

guccacagcg cgguuacgcu gcccucccau ucuacccgga aacuucagac acgcagccaa      120

accuggcugg aguccagaga auacacaaaa caccucauuc ggguggagaa cuggaucuuu      180

cgcaauccug gcuucgcucu ggccgccgcu gccauugcuu ggcuucuggg gucuagcacc      240

ucccagaagg ugaucuaucu cgucaugauu cugcugaucg ccccggcgua cucuaucaga      300

ugcauuggag ugagcaaccg ggauuuuguu gaaggcaugu ccgggggaac auggguggac      360

gucgugcucg agcauggcgg guguguuacc gugauggccc aggauaaacc cacggucgac      420

aucgaacugg ugaccacaac cgugagcaau auggcugagg uccgcucuua uugcuacgag      480

gccuccauua gcgauauggc uucugacucc agauguccua cacagggaga agccuaucuu      540

gauaagcaaa gcgacaccca guacguuugc aaacggacgc ugguggaucg cggcuggggc      600

aacgggugug gacucuucgg caaggggucu cuggugacau gcgcgaaguu ugccuguucc      660

aaaaagauga ccggaaaaag cauccagccc gagaaucuug aauauagaau uaugcugucc      720

guccacggcu cucagcauag cgggaugauc gugaacgaca caggacacga gaccgaugag      780

aaucgggcua agguugaaau uaccccuaac agcccccgcg ccgaggcuac acucggcggc      840

uucggguccc ugggacugga cugcgaaccu agaacgggcc ucgauuuuuc ugaccuguac      900

uaccuuacca ugaauaacaa acacuggcug gugcauaagg agugguucca cgacaucccg      960

cuccccuggc augccggggc cgauacagga accccucacu ggaauaacaa ggaagcucug     1020

gucgaguuua aagacgcgca cgccaagcgg caaacagugg uugugcuugg cucccaggag     1080

ggggcugucc auaccgcccu ggccggagcu cucgaagccg agauggaugg cgcgaaaggc     1140

cgccugagcu cugggcaccu gaaguguaga cucaaaaugg acaagcugcg gcuuaaagga     1200

gugagcuauu cccugugcac ggcugccuuc acauuuacca agauccccgc cgaaacccuc     1260

cauggcacag ucaccgugga gguucaguac gcugggacag auggaccuug uaaggugccc     1320

gcccagaugg cugucgacau gcaaacgcug accccggugg gcagacuuau uacagccaau     1380

ccuguuauca ccgaaucuac agagaacagc aaaaugaugc uggagcucga ucccccuuuc     1440

ggggacuccu auauugugau cggcgucgga gaaaagaaaa uuacccacca uuggcaccgc     1500

agcggcucua cgaucgggaa ggcguuugag gccaccgugc ggggagcuaa aagaauggcc     1560

gugcugggcg auacagcuug ggacuucggg uccguuggag gcgcccugaa uagccucggg     1620

aagggcaucc accagauuuu cggagcggcc uuuaagucuc uguucggcgg gauguccugg     1680

uuuagccaga uccuuauugg aacccugcuc auguggcugg gccugaacac aaagaacggg     1740

uccaucucuc uuaugugccu cgcucuggga ggcguccuga uuuuccucag caccgccgug     1800

uccgcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10237
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10237
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccgcc guuacccugc ccagccacuc cacacggaaa      120

cuucagacgc gcucucaaac cuggcuggag agcagagaau acacaaagca ucucauccgg      180

guggagaacu ggauuuuccg caauccuggc uuugcucugg ccgcugccgc gaucgccugg      240

cuucuggggu ccucuaccag ccagaaagug auuuaucucg ucaugauccu gcugaucgcu      300

ccggccuacu ccauuagaug uaucggagug agcaaccggg acuucguuga aggcaugucu      360

gggggaacau ggguggauau uguccucgag cacggcggcu gcgugaccgu uauggcucag      420

gacaagccca caguggauau cgaacugguc accacgaccg uguccaauau ggccgaggug      480

cgcagcuauu guuacgaggc gucuauuucc gacauggcca gcgauucuag augcccuaca      540

cagggggagg cuuaucuuga caaacaaucc gauacccagu acgucuguaa gcggacacug      600

guugaccgcg gauggggcaa cgggugcgga cucuuuggca aggggagccu ggugaccugu      660

gccaaauucg cuugcuccaa gaaaaugacg ggaaagucua uccagcccga aaaucuugaa      720

uauagaauua ugcugagcgu gcauggcucc cagcacagcg gcaugaucgu caacgauaca      780

gggcaugaga ccgacgagaa ucgggccaaa guggaaauca caccuaacuc uccccgcgcc      840

gaggcuaccc ucggaggcuu ugggucccug ggacuggauu gugaaccuag aaccggccuc      900

gacuucagcg aucuguacua ccuuacaaug aauaacaagc acuggcuggu ucacaaggag      960

ugguuucaug acauuccgcu ccccuggcac gcgggggccg acacgggaac cccucauugg     1020

aauaacaaag aagcucuggu ggaguucaag gaugcccacg ccaaacggca aacagucgug     1080

guucuuggcu cucaggaggg cgcugugcac accgcccugg cgggggcucu cgaagccgag     1140

auggacggag ccaagggccg ccugagcucc gggcaucuga aaugcagacu caagauggau     1200

aaacugcggc uuaagggagu cucuuauagc cuguguacag cugccuuuac cuucacgaag     1260

auccccgcug aaacacucca cggcaccgug accgucgagg ugcaguacgc cgggacagac     1320

ggcccuugca aaguucccgc gcagauggcc guggauaugc aaacccugac accggucgga     1380

agacuuauua cggcuaaucc ugugaucacc gaauccacag agaacagcaa gaugaugcug     1440

gagcucgauc ccccuuuugg cgacucuuau auuguuaucg gggugggaga gaaaaagauc     1500

acccaucacu ggcaucgcuc cggcagcaca auugggaaag ccuucgaagc uaccguccgg     1560

ggagccaaga gaauggcggu gcugggcgac acggccuggg auuuuggguc ugugggcgga     1620

gcucucaauu cccugggcaa ggggauccac cagauuuucg gagccgcuuu caaaagccug     1680

uuuggcggga uguccugguu cucucagauc cuuauuggaa cccugcucau guggcugggc     1740

cugaacacaa agaacgggag caucucccuu augugccucg cccugggcgg aguucugauc     1800

uuucucucua ccgccgucag cgcuugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10238
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10238
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccgcc guuacccugc ccagccacuc cacacggaaa      120

cuucagacgc gcucucaaac cuggcuggag agcagagaau acacaaagca ucucauccgg      180

guggagaacu ggauuuuccg caauccuggc uuugcucugg ccgcugccgc gaucgccugg      240

cuucuggggu ccucuaccag ccagaaagug auuuaucucg ucaugauccu gcugaucgcu      300

ccggccuacu ccauuagaug uaucggagug agcaaccggg acuucguuga aggcaugucu      360

gggggaacau ggguggaugu cgugcucgag cacggcggcu gcguuaccgu gauggcucag      420

gacaagccca cagucgauau ugaacuggug accacgaccg uguccaauau ggccgagguc      480

cgcagcuauu guuacgaggc gucuaucucc gacauggcca gcgauucuag augcccuaca      540

cagggggagg cuuaucuuga caaacaaucc gauacccagu acguuuguaa gcggacacug      600

guggaccgcg gauggggcaa cgggugcgga cucuuuggca aggggagccu ggugaccugu      660

gccaaauucg cuugcuccaa gaaaaugacg ggaaagucua uucagcccga aaaucuugaa      720

uauagaauca ugcugagcgu ccauggcucc cagcacagcg gcaugauugu gaacgauaca      780

gggcaugaga ccgacgagaa ucgggccaaa guugaaauca caccuaacuc uccccgcgcc      840

gaggcuaccc ucggaggcuu ugggucccug ggacuggauu gugaaccuag aaccggccuc      900

gacuucagcg aucuguacua ccuuacaaug aauaacaagc acuggcuggu gcacaaggag      960

ugguuucaug acaucccgcu ccccuggcac gcgggggccg acacgggaac cccucauugg     1020

aauaacaaag aagcucuggu cgaguucaag gaugcccacg ccaaacggca aacagugguu     1080

gugcuuggcu cucaggaggg cgcuguccac accgcccugg cgggggcucu cgaagccgag     1140

auggacggag ccaagggccg ccugagcucc gggcaucuga aaugcagacu caagauggau     1200

aaacugcggc uuaagggagu gucuuauagc cuguguacag cugccuuuac cuucacgaag     1260

auucccgcug aaacacucca cggcaccguc accguggagg uucaguacgc cgggacagac     1320

ggcccuugca aagugcccgc gcagauggcc gucgauaugc aaacccugac accgguggga     1380

agacuuauca cggcuaaucc uguuauuacc gaauccacag agaacagcaa gaugaugcug     1440

gagcucgauc ccccuuuugg cgacucuuau aucgugauug gggucggaga gaaaaagauc     1500

acccaucacu ggcaucgcuc cggcagcaca aucgggaaag ccuucgaagc uaccgugcgg     1560

ggagccaaga gaauggcggu gcugggcgac acggccuggg auuuuggguc uguuggcgga     1620

gcucucaauu cccugggcaa ggggauucac cagaucuucg gagccgcuuu caaaagccug     1680

uuuggcggga uguccugguu cucucagauu cuuaucggaa cccugcucau guggcugggc     1740

cugaacgcca agaacgggag cauuucccuu augugccucg cccugggcgg aguccugauc     1800

uuucucucua cagcugugag cgcgugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10239
<211>  2017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10239
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccgcc guuacccugc ccagccacuc cacacggaaa      120

cuucagacgc gcucucaaac cuggcuggag agcagagaau acacaaagca ucucaucaaa      180

guggagaacu ggauuuuccg gaauccuggc uuugcucugg uggccgucgc uaucgccugg      240

cuucuggggu ccucuaccag ccagaaggug auuuaucucg uuaugauccu gcugaucgcg      300

ccggccuacu ccauucgcug uaucggagug agcaacagag acuucgucga aggcaugucu      360

gggggaacau ggguggaugu ugugcucgag cacggcggcu gcgucaccgu gauggcucag      420

gacaaaccca caguggauau ugaacugguc accacgaccg uuuccaauau ggccgaggug      480

cggagcuauu guuacgaggc uucuaucucc gacauggcca gcgauucucg cugcccuaca      540

cagggggaag cguaucuuga caagcaaucc gauacccagu acguguguaa gagaacacug      600

gucgaccggg gauggggcaa cgggugcgga cucuuuggca aagggagccu ggugaccugu      660

gccaaguuca cgugcuccaa aaagaugaca ggaaaaucua uucagcccga gaaucuugaa      720

uaucgcauca ugcugagcgu ucauggcucc cagcacagcg gcaugauugg guacgagacc      780

gaugaggaca gagcuaaggu ggaagucaca ccuaacucuc cccgggccga ggcuacccuc      840

ggaggcuuug ggucccuggg acuggauugu gaaccucgca ccggccucga cuucagcgau      900

cuguacuauc uuacaaugaa uaacaagcau uggcuggugc acaaagagug guuucacgac      960

aucccgcucc ccuggcaugc cggggccgac acgggaaccc cucacuggaa uaacaaggaa     1020

gcucugguug aguucaaaga ugcgcaugcc aagagacaaa caguggucgu gcuuggcucu     1080

caggagggcg cuguccacac cgcccuggcc ggggcucucg aagccgagau ggacggagcg     1140

aaaggccggc uguuuagcgg gcaccugaag ugccgccuca aaauggauaa gcugagacuu     1200

aagggagugu ccuacucucu guguacagcu gccuucaccu uuacgaaagu ucccgccgaa     1260

acacuccaug gcaccgugac cgucgaggug caguaugcug ggacagacgg cccuugcaag     1320

auccccguuc agauggccgu ggauaugcaa acccugacac cggucggacg gcuuauuacg     1380

gcuaauccug ugaucaccga aagcacagag aacuccaaaa ugaugcugga gcucgauccc     1440

ccuuucggcg acagcuacau ugugaucggg guuggagaca agaaaauuac ccaccauugg     1500

cacagaucug gcuccacaau cgggaaggcc uuugaagcga ccguccgcgg agccaagcgg     1560

auggcugugc ugggcgauac ggccugggac uucgggagcg ugggcggagu cuucaauucu     1620

cugggcaaag ggauccauca gauuuuugga gcugccuuca agucccucuu uggcgggaug     1680

agcugguucu cccagauccu gauuggaacc cuucuggugu ggcucggccu gaacaccaaa     1740

aaugggucua ucagccugac augccuugcg cucggcggag uuaugauuuu ucuguccaca     1800

gccgugucug cuugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     1860

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1980

ccccccccca aaggcucuuu ucagagccac cagaauu                              2017


<210>  10240
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10240
gggagaaagc uuaccauggg caagagaagc gccgggucca ucauguggcu ggcuucucuc       60

gccgugguca uugcgugcgc uggagccgcc guuacccugc ccagccacuc cacacggaaa      120

cuucagacgc gcucucaaac cuggcuggag agcagagaau acacaaagca ucucauccgg      180

guggagaacu ggauuuuccg caauccuggc uuugcucugg ccgcugccgc gaucgccugg      240

cuucuggggu ccucuaccag ccagaaagug auuuaucucg ucaugauccu gcugaucgcu      300

ccggccuacu ccauuagaug uaucggagug agcaaccggg acuucguuga aggcaugucu      360

gggggaacau ggguggaugu cgugcucgag cacggcggcu gcguuaccgu gauggcucag      420

gacaagccca cagucgauau ugaacuggug accacgaccg uguccaauau ggccgagguc      480

cgcagcuauu guuacgaggc gucuaucucc gacauggcca gcgauucuag augcccuaca      540

cagggggagg cuuaucuuga caaacaaucc gauacccagu acguuuguaa gcggacacug      600

guggaccgcg gauggggcaa cgggugcgga cucuuuggca aggggagccu ggugaccugu      660

gccaaauucg cuugcuccaa gaaaaugacg ggaaagucua uucagcccga aaaucuugaa      720

uauagaauca ugcugagcgu ccauggcucc cagcacagcg gcaugauugu gaacgauaca      780

gggcaugaga ccgacgagaa ucgggccaaa guugaaauca caccuaacuc uccccgcgcc      840

gaggcuaccc ucggaggcuu ugggucccug ggacuggauu gugaaccuag aaccggccuc      900

gacuucagcg aucuguacua ccuuacaaug aauaacaagc acuggcuggu gcacaaggag      960

ugguuucaug acaucccgcu ccccuggcac gcgggggccg acacgggaac cccucauugg     1020

aauaacaaag aagcucuggu cgaguucaag gaugcccacg ccaaacggca aacagugguu     1080

gugcuuggcu cucaggaggg cgcuguccac accgcccugg cgggggcucu cgaagccgag     1140

auggacggag ccaagggccg ccugagcucc gggcaucuga aaugcagacu caagauggau     1200

aaacugcggc uuaagggagu gucuuauagc cuguguacag cugccuuuac cuucacgaag     1260

auucccgcug aaacacucca cggcaccguc accguggagg uucaguacgc cgggacagac     1320

ggcccuugca aagugcccgc gcagauggcc gucgauaugc aaacccugac accgguggga     1380

agacuuauca cggcuaaucc uguuauuacc gaauccacag agaacagcaa gaugaugcug     1440

gagcucgauc ccccuuuugg cgacucuuau aucgugauug gggucggaga gaaaaagauc     1500

acccaucacu ggcaucgcuc cggcagcaca aucgggaaag ccuucgaagc uaccgugcgg     1560

ggagccaaga gaauggcggu gcugggcgac acggccuggg auuuuggguc uguuggcgga     1620

gcucucaauu cccugggcaa ggggauucac cagaucuucg gagccgcuuu caaaagccug     1680

uuuggcggga uguccugguu cucucagauu cuuaucggaa cccugcucau guggcugggc     1740

cugaacacaa agaacgggag cauuucccuu augugccucg cccugggcgg aguccugauc     1800

uuucucucua ccgccgugag cgcuugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10241
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10241
gggagaaagc uuaccauggc aauaagcgga guaccaguuu uagguuucuu uauaauagcu       60

gucuuaaugu cagcacagga aucaugggca guaacuagaa gagggucagc cuauuauaug      120

uaccuagaua gaaaugacgc uggugaagcg auaaguuuuc caaccacuuu aggcaugaau      180

aaaugcuaua uacaaauaau ggaccuugga cauaugugcg augcuacaau gucauacgaa      240

ugcccaaugu uagaugaagg uguagaacca gaugacguag auugcuggug uaauacaacg      300

aguacauggg uaguauaugg aacuugccau cauaagaaag gagaagcaag aagaucaagg      360

agagcaguua cauuaccauc ucauucuacu agaaaacuac aaaccagauc ucaaacuugg      420

uuagaaagua gagaauacac aaaacaucua auaagaguag aaaauuggau cuuuagaaau      480

ccaggauuug cauuagcagc agcagcaaua gcauggcuau uaggaagcuc aacaagucaa      540

aaaguaauau acuuaguaau gauacuucua auagcaccag cauauucaau aagauguaua      600

gguguaucaa auagagauuu ugucgaagga augucaggug guacaugggu agauauaguc      660

uuagaacacg gaggaugugu cacaguuaug gcucaagaua aaccaacagu agauauugag      720

cuaguaacaa cgacaguauc aaacauggcc gaagucagau cauacuguua ugaagcauca      780

auaagcgaca uggcaaguga uaguagaugu ccgacacaag gagaagcaua ucuagacaaa      840

caaucagaua cacaguaugu augcaaaaga acauuaguag auagaggcug gggaaacggu      900

ugcggauuau uugguaaagg cagccucgua acaugcgcaa aauuugcaug cucgaagaaa      960

augacaggua aaaguauaca accagaaaac cuagaauaca gaaucauguu aaguguucau     1020

gguagucaac auaguggaau gauaguuaac gauacaggac acgaaacuga ugaaaauaga     1080

gcaaaaguag aaauaacacc uaauucucca agagcagaag caacacuagg gggauuuggu     1140

ucauuaggau uagacuguga accaagaaca gguuuagauu uuucagauuu auauuaccuc     1200

acaaugaaua acaaacauug guuaguacau aaagaauggu uucaugauau uccauugccu     1260

uggcaugccg gugcagacac aggcacacca cauuggaaua auaaggaagc acuuguggaa     1320

uuuaaagaug cacaugcaaa aagacaaaca guuguaguac ucgguucaca agaaggagca     1380

guacacaccg cauuagcagg cgcacucgag gcagaaaugg auggggcaaa agggagacua     1440

aguaguggac aucugaaaug cagauuaaaa auggacaaac uacgacuaaa aggaguauca     1500

uauagucuuu gcacagccgc guucacguuc acuaaaauac cugcugaaac auuacacgga     1560

acgguaacug uagaaguaca auaugcagga acagauggac caugcaaagu uccugcgcaa     1620

auggcagucg acaugcaaac auuaacacca guagggaggu uaauuacagc uaauccaguu     1680

auaacagaaa guacagagaa uaguaaaaug augcuagaau uagauccacc uuuuggggac     1740

ucuuacauag uaauaggagu aggagaaaag aaaauaaccc aucauuggca ucgaagugga     1800

aguacaauag gaaaagcauu ugaggcaaca guaagaggag caaaaagaau ggcaguacuu     1860

ggagauacag ccugggacuu cggcaguguc ggaggagcac uaaauaguuu aggaaaaggu     1920

auacaucaaa uuuuuggagc cgccuuuaaa ucauuauucg gagggauguc cugguuuagu     1980

caaauauuaa uuggcacauu auuaaugugg cuaggauuaa auaccaaaaa uggcucaaua     2040

ucauuaaugu guuuagcguu agguggagua cuaaucuuuu ugagcacggc agucagugcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10242
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10242
gggagaaagc uuaccauggc uauaagcgga guaccagucc uagguuucuu uauaaucgcu       60

gucuuaaugu cagcacaaga aucgugggcc guaaccagaa gaggaagugc auauuauaug      120

uauuuagacc gaaaugaugc aggcgaagcg auaucauuuc caacgacauu aggaaugaau      180

aaauguuaua uacaaauaau ggauuuagga cacacaugcg augcaaccau gucuuacgaa      240

uguccaaugu uagacgaagg aguagaacca gaugacguag acuguuggug uaauacaacc      300

aguaccuggg uaguuuacgg aacaugucau cacaagaaag gagaagcaag aagaaguaga      360

cgagcaguaa ccuugccauc acauucaaca agaaaauuac aaacaagauc acaaacaugg      420

uuagaaaguc gagaauauac aaaacaucua auaagaguag aaaacuggau auuuaggaac      480

ccuggauuug cacucgcagc agcugcaauu gcaugguugc uaggaagcuc uacuucccaa      540

aaaguuauuu auuuaguaau gauauuauua auagcaccag cauauucaau uagauguauc      600

gguguaucua auagagacuu uguagaagga augagcggag guacuugggu agaugucgua      660

uuagaacaug gaggaugugu aacaguaaug gcccaagaua aaccaacggu agacauagaa      720

uuagucacua caacuguuuc aaauauggcu gaaguuagau cauacuguua ugaagcaagu      780

auaucagaua uggccaguga cuccagaugu ccaacccaag gagaagcaua uuuagacaaa      840

caaagugaca cacaauaugu augcaaaaga acucuaguag auagaggcug gggaaaugga      900

ugcggauuau uuggaaaggg gaguuuagua acaugcgcaa aauuugcaug cucaaagaaa      960

augacaggaa aaaguaucca accagaaaau uuagaauaua gaauaauguu aucaguacau     1020

ggaucgcagc acagcggaau gauuguuaac gacacaggac augaaacaga ugaaaacaga     1080

gcaaaaguug aaauuacacc aaacucacca agagcagaag cgacauuagg aggauuugga     1140

ucacuagguu uagauuguga acccagaacu ggacuugauu ucagugacuu auauuaucuu     1200

acgaugaaua auaaacacug gcuaguucac aaagaauggu uucaugauau accacuucca     1260

uggcaugcag gagcagauac cggcacaccu cacuggaaca auaaagaggc ccuaguagaa     1320

uuuaaagacg cucaugcaaa aagacagacu guugugguau uaggcucaca agaaggagca     1380

guucacacug cgcuagcagg ggccuuagag gcagaaaugg acggcgcaaa agguagacuc     1440

ucaagcggac auuuaaaaug cagacugaaa auggauaaau uacgacuaaa aggaguuagu     1500

uauagccuau guacugccgc auuuacuuuc acaaaaauac cagcagaaac acugcaugga     1560

acuguaacgg uagaaguaca auacgccggu acagacggac cuuguaaagu accagcacaa     1620

auggccguag auaugcaaac acuaacuccu guugguagac uaauaacugc aaauccugua     1680

auaacagaaa gcacagaaaa uucaaaaaug auguuagaau uagauccacc uuuuggagau     1740

ucauauauag uaaucggggu uggagaaaag aaaauuacac accauuggca ucgaucagga     1800

ucaacaaucg guaaagccuu ugaagcgaca guaagaggug caaaaagaau ggcaguauua     1860

ggagauacag caugggauuu ugguucggua ggaggagcau uaaauucacu aggaaaaggu     1920

auucaccaaa uauuuggagc agccuuuaaa aguuuauuug gaggaaugag uugguuuagu     1980

caaauuuuga uaggaaccuu auuaaugugg cuuggacuca acgcaaagaa cggaucaauu     2040

agcuuaaugu gucuagcguu aggaggaguu uugauauucc uaucaacagc aguaagugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10243
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10243
gggagaaagc uuaccauggc cauaucagga guaccaguau uagguuuuuu cauaauagca       60

guacucaugu cagcacaaga aucaugggcg auaacaagaa gaggaucagc auauuacaug      120

uauuuagaua gaucagaugc gggaaaagca auuuccuuug caacaacauu aggugugaac      180

aaaugccaug uacaaauuau ggaucuuggu cacaugugcg acgcaacaau gucguacgaa      240

ugcccuaugc uugacgaagg aguugaacca gaugacgucg auuguuggug uaacacaacc      300

ucaacauggg uugucuacgg uacuugucau cauaaaaaag gggaagcaag gagaucaaga      360

agagcuguaa cacuaccaag ucauucaacu agaaaauuac aaacaagaag ccaaacaugg      420

cuagaaucuc gagaauauac caaacacuua auaaaaguag aaaacuggau auuuaggaau      480

ccaggauucg cauuagucgc cguagccauu gccugguuac ugggaucuag uacaagccaa      540

aaagucauuu accuaguaau gauacuuuua auagcaccag cauacucaau ucgguguaua      600

ggugucagua acagggauuu uguugaaggu augaguggag gaacaugggu agauguugua      660

cucgaacacg gcggaugugu cacaguaaug gcacaagaua aaccaacugu agacauagaa      720

cuaguaacaa caacuguauc aaacauggcg gaaguuagau cauacuguua ugaagcaagu      780

auaucagaua uggcaucaga uucacgcugc ccaacacaag gcgaagcaua uuuagauaaa      840

caaagcgaua cacaauaugu augcaaacga acacuaguag acagaggcug gggcaaugga      900

ugcggauuau ucggcaaagg aagucuugua accugugcaa aauuuacaug cucaaaaaaa      960

augacaggua aaaguauaca accagaaaau cuagaauauc gaauaauguu aagcguacau     1020

ggaucacaac auucuggaau gauuggauau gaaacggaug aagauagagc aaaaguagaa     1080

guaacaccaa auuccccaag agcagaagcc acacuaggug gcuuuggauc ucuaggcuua     1140

gauugugaac caagaacugg auuagauuuu ucagauuuau auuauuugac aaugaauaau     1200

aaacauuggu ugguacauaa agaaugguuu cacgauauac cuuuaccaug gcaugcugga     1260

gcagacacag gaaccccaca uuggaacaau aaagaagcau uagucgaguu caaagaugcc     1320

caugcaaaaa gacaaacggu aguaguucua ggaucccaag aaggagcggu acauacagca     1380

cucgcaggag cacuagaagc cgaaauggau ggugcuaaag gaagauuauu uucaggacau     1440

cuuaaaugua gauuaaaaau ggauaaauua agacuuaaag gaguaucuua uagucucugu     1500

acugcggcuu uuacauuuac aaaaguacca gcagaaacgu uacaugguac aguaacagua     1560

gaaguacaau acgcaggaac agacggucca uguaaaauuc cuguacaaau ggcaguagau     1620

augcaaacuc ugacaccagu agguagauua auaacagcua auccuguaau aacagaguca     1680

accgagaacu caaaaaugau gcuagaauua gauccaccuu uuggagauuc auauauaguc     1740

auaggcguag gagacaaaaa aauaacacau cacuggcaca gaucaggaag uacuauaggg     1800

aaggcauuug aagcaacggu aagaggcgca aaaagaaugg caguauuagg agauaccgca     1860

ugggauuuug gaaguguugg ugguguguuu aacucuuuag ggaaagggau acaucaaaua     1920

uuuggagcag cauuuaaaag cuuguuugga gguaugucau gguuuucaca aauacuaaua     1980

ggaacauugc uaguaugguu aggauuaaau acuaaaaaug gaagcauaag cuuaacaugc     2040

uuagcauuag guggaguaau gauauuuuua ucaacagcag uaucagcaug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10244
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10244
gggagaaagc uuaccauggc uauuucuggg guuccaguau uaggauucuu uauaauagca       60

guauuaaugu cagcacaaga aaguugggca guaacaagaa gaggaagugc auacuauaug      120

uauuuggaca gaaaugacgc uggagaagcc auaaguuuuc caacaacauu gggcaugaac      180

aaauguuaua uacaaauaau ggauuuagga cauaugugug augcaacaau gucauacgaa      240

uguccuaugc uagaugaagg agucgaacca gacgauguag auuguuggug caauacaacu      300

ucaacauggg uaguuuaugg aaccugucau cauaagaaag gggaagcaag aaggaguaga      360

agggcaguaa cuuuacccuc ucauagcaca agaaaauuac aaacaagauc acaaacuugg      420

cucgaaagca gagaauauac uaaacauuua auaagaguag aaaauuggau uuucagaaau      480

ccaggauuug cucuagcagc agcagcaaua gcaugguuac uugguuccag uacaagucaa      540

aaaguaaucu accuuguaau gauacuucuu auugcuccag cguauucaau aagguguauu      600

ggaguaagua acagagacuu cguagaaggu augucuggag gcacgugggu agacguagua      660

uuagaacacg gugggugugu aacuguaaug gcucaagaua aaccaacugu ugauaucgaa      720

uuagugacaa caacaguuuc aaacauggcu gaaguacgaa gcuacugcua cgaagcauca      780

auaagugaua uggcaaguga uagucgaugc ccaacacaag gagaagcaua ucuagacaaa      840

caaagugaua cacaauaugu auguaaaaga acacuuguag auagaggaug gggaaauggu      900

ugugggcuau uuggcaaagg gagcuuaguc accugugcaa aauuugcuug uagcaagaaa      960

augacaggaa aaagcauaca accagaaaau cuagaauaua gaauaauguu aaguguccau     1020

ggaucacaac auaguggaau gauaguaaau gacaccggac acgaaacuga cgaaaaucga     1080

gcaaaagugg aaauaacacc aaauaguccc agggccgaag cuacauuagg agguuuugga     1140

ucacuaggac uggauuguga acccaggaca ggguuagauu ucagcgauuu auauuauuua     1200

acaaugaaca auaaacauug guugguacau aaagaauggu uucaugauau accauuaccc     1260

uggcaugcug gugcugauac aggcacaccu cauuggaaca auaaagaagc auuaguagaa     1320

uuuaaagaug cgcaugcaaa aagacaaaca guugucguuu uaggaaguca agaaggcgca     1380

guacauacug cucuagcagg agcauuagaa gcugaaaugg auggagcaaa aggcagacug     1440

ucaaguggac auuuaaaaug ucgacuaaaa auggauaaau ugcgauuaaa aggaguauca     1500

uauuccuuau gcacugcagc uuuuacauuu acaaaaauac cugcagaaac guuacaugga     1560

acaguuacag uagaaguuca auacgccgga acagauggac cauguaaagu accagcacaa     1620

auggcaguag acaugcaaac auuaacaccu gucggacgac uaaucacagc aaauccaguu     1680

auuacagaau caacagaaaa uaguaaaaug augcuagaau uagauccacc auucggagau     1740

ucauacauag uaauaggagu uggagaaaag aaaauaacac accauuggca ucgaagcgga     1800

aguacaauag gaaaagcguu cgaagcuaca guccguggag caaaaagaau ggcaguauua     1860

ggagauaccg caugggauuu uggaagugua ggcggagcau uaaauaguuu aggaaaaggu     1920

auacaccaaa ucuuuggagc agcauucaaa ucacuauuug gaggaaugag cugguuuucg     1980

cagauacuga uagguacuuu auuaaugugg cuaggauuaa auacgaaaaa cgguagcaua     2040

ucauugaugu guuuagcacu cgguggagua cuaauauuuu uaucuacagc uguauccgcg     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10245
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10245
gggagaaagc uuaccaugga uuggacaugg auuuuauuuc uagucgcagc agcuacaaga       60

guacacuccg uaacaagaag aggaucggca uacuauaugu auuuagacag aaacgaugca      120

ggggaagcaa uaucauuucc aacaacucuu gguaugaaua aauguuacau acagauaaug      180

gauuuaggac acaugugcga ugccacuaug ucguacgagu gcccaauguu agacgaaggg      240

guggagccgg augauguaga uugcuggugu aacacuaccu cuacaugggu agucuaugga      300

acaugucacc acaagaaagg agaagcaaga agaaguagac gagcaguaac cuuaccaagu      360

cauucaacaa gaaaauuaca aacaagauca caaacauggu uagaaucaag ggaauauaca      420

aaacauuuaa uuagaguaga aaacuggaua uuuagaaauc cugguuuugc uuuggcagca      480

gccgcuaucg cuugguuauu aggaagcagc acaucacaaa aaguaauaua uuugguaaug      540

auuuuacuca uagcccccgc guauaguauu cgauguauag guguaucuaa uagagacuuc      600

guagaaggaa ugucaggagg aacuugggua gacaucguau uagaacacgg aggaugugua      660

acaguaaugg cucaagacaa accgacagua gauauagaau ugguaacaac aacuguuucu      720

aauauggcag agguacgauc auacuguuau gaagcaagca ucagugauau ggcuucagac      780

agccgauguc caacacaagg ugaagcauac uuagauaaac aaagcgacac acaauaugua      840

ugcaaaagaa cguuaguaga uagaggcugg ggaaaugguu gcggguuauu cggaaaagga      900

ucauugguaa caugcgcaaa auuugcaugu ucaaagaaaa ugacuggaaa aucuauacaa      960

ccugaaaauc uagaauauag gauaauguua aguguucaug gaucucaaca cucaggaaug     1020

auaguaaaug acacaggaca cgaaacggau gaaaauagag ccaaaguaga aauaacacca     1080

aauucaccga gagcagaagc gacauuggga ggauuugggu cacuaggauu agauugugaa     1140

ccgagaaccg gguuagauuu uucugaucuc uauuaucuaa caaugaauaa uaaacauugg     1200

uuaguacaca aagaaugguu ccaugauauu ccauuaccuu ggcacgcagg agccgauaca     1260

gguacuccac acuggaacaa uaaagaagca uuaguagaau uuaaagacgc acaugcaaaa     1320

agacaaacag uaguaguauu aggaagucaa gaaggcgcag uacacaccgc ucuagcagga     1380

gccuuagaag cagaaaugga uggagcaaag gguagauugu cuucaggcca ucuaaaaugu     1440

agacuaaaaa uggauaaacu aagauuaaaa ggaguaagcu acucuuuaug cacagcagca     1500

uuuacguuua caaaaauacc agcagaaacc uuacauggaa cuguuacugu agaaguacaa     1560

uaugcaggaa cagauggacc auguaaaguu ccagcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccag uaggaagauu aauuacagca aauccaguaa uuacagaauc aaccgaaaac     1680

ucaaaaauga uguuagaauu agacccaccu uucggagacu cauauauagu aauaggagua     1740

ggagaaaaga aaauuacaca ccauuggcac cgauccggau caacaauagg aaaagcauuc     1800

gaagcuacag uacgaggcgc aaaaaggaug gcagucuuag gagauacagc augggauuuu     1860

ggaagcguag gaggagcacu uaacuccuua gguaaaggca uacaucaaau auuuggagca     1920

gcguuuaaau cauuauuugg ggguaugucu ugguuuagcc aaauacucau agguaccuua     1980

uuaauguggc uaggacuuaa uacuaaaaac gguucaauuu cauuaaugug uuuagccuua     2040

ggagguguuu uaauauuucu uaguacagca guuucagcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10246
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10246
gggagaaagc uuaccaugga uuggacaugg auauuauuuu uaguggcagc agcaacaaga       60

gugcacucag ugaccaggag aggaagugca uauuauaugu auuuagauag aaaugacgcg      120

ggagaagcaa uaucauuccc aacuacguua ggaaugaaua aauguuauau acaaauaaug      180

gauuuagguc auacauguga ugcaacaaug ucuuacgaau guccgauguu agacgaagga      240

guagaaccag augacguaga cugcuggugc aauacaacaa guacuugggu aguauacgga      300

acaugccauc acaaaaaagg agaagcuaga agaucucgaa gggcaguaac uuuaccaagu      360

cauucuacac gaaaauugca aaccagguca caaacauggu uagaaucaag ggaauacaca      420

aaacaucuga uaagaguaga aaacuggaua uuuagaaauc ccggguuugc auuagcugca      480

gcagcaauag cauggcuuuu agguucauca acaucucaaa aaguaauaua ucuugugaug      540

auacucuuaa uugcaccugc uuacaguauu agguguauag gugucucaaa uagagacuuu      600

gucgaaggga ugaguggugg uacauggguu gauguugucu uggaacaugg uggaugugua      660

acaguaaugg cacaagauaa accaaccgua gauauagaau uagucacaac gacaguaucu      720

aacauggcag aaguuagauc uuauugcuau gaagcgucaa uaucugauau ggccucggac      780

ucaagaugcc caacacaagg cgaagcauau cuagauaaac aaucagauac ccaguaugua      840

uguaaaagaa cauuaguuga uagagguugg ggaaaugguu gcggacuauu uggcaaagga      900

ucauuaguaa caugugcaaa auucgcaugu agcaaaaaaa ugacaggaaa aucaauucaa      960

ccagaaaauu uggaguauag aauaaugcua agugugcacg gaagccagca uaguggaaug     1020

auuguaaaug auacaggaca ugaaacugac gagaauagag cgaaaguaga aaucacacca     1080

aauucaccaa gagcugaagc uacacuagga ggauuuggua guuuaggccu cgauugugaa     1140

ccaagaacag gauuagacuu cuccgaucua uauuauuuaa caaugaauaa uaaacacugg     1200

cuaguucaua aagaaugguu ucaugauaua ccuuuaccuu ggcaugcagg agcagauaca     1260

ggaacuccuc auuggaacaa uaaagaagca cuaguagaau uuaaagaugc acacgcaaaa     1320

agacaaacag uagucguacu aggaagucaa gaaggagcag uucauacagc uuuagcagga     1380

gcauuagaag cagaaaugga uggagcaaaa gggagauuau caucaggaca ccuaaaaugu     1440

agauuaaaaa uggauaaauu aagauuaaaa ggaguaucau acucauugug uacagcagca     1500

uuuacauuua caaaaauacc agcagaaaca uuacauggaa cagucacggu ugaaguacaa     1560

uacgccggaa ccgacggacc augcaaagua ccugcacaaa uggcaguaga uaugcaaaca     1620

cuaacaccag uaggacgacu aauaaccgcg aauccaguaa uaacagaaag uacagaaaau     1680

agcaaaauga uguuagaacu agauccucca uuuggagauu cauacauagu aauuggaguc     1740

ggggaaaaaa agauuacuca ccacuggcau cgaucgggca guacaauagg aaaagcauuu     1800

gaagcaacag uacgcggagc aaaaagaaug gcaguacuag gagacaccgc augggauuuc     1860

gguucaguag guggagcauu aaauucauua gguaaaggaa uacaccaaau auucggagcc     1920

gccuuuaaaa guuuguuugg cggaaugagu ugguuuagcc aaauauugau cggaaccuua     1980

cuaauguggc uggguuuaaa ugcaaaaaau gguagcauaa gucuaaugug cuuagcacug     2040

gguggaguac uaauauuuuu aucaacggca guaucagcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10247
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10247
gggagaaagc uuaccaugga uuggaccugg auauuauuuu uaguagcagc agcuacuagg       60

gugcauucaa uaacaagaag aggaucagcu uacuauaugu auuuagacag aagcgacgca      120

ggaaaagcaa ucagcuuugc uacaacacua gguguaaaua aaugucaugu acaaauaaug      180

gauuuaggac auauguguga ugcaacaaug aguuacgaau guccuauguu agaugaaggu      240

guggaaccug acgauguaga uuguuggugc aauacuacua gcacuugggu agucuauggc      300

acaugucauc auaagaaagg cgaagcaagg agaaguagac gugcaguaac auugccaucu      360

cacaguacac gaaaauuaca aacuagauca cagacauggc uugaaucaag agaauauaca      420

aaacacuuaa uaaaaguaga aaauuggaua uucagaaauc cuggguucgc acuuguggcu      480

gucgcuauag cuugguuacu aggaaguagu acgagucaaa aaguuauaua ucuuguuaug      540

auauuacuaa uagcaccagc auacaguaua agauguauag ggguuucuaa cagagacuuu      600

guugaaggaa ugucaggugg aacaugggua gacguaguau uagaacacgg aggaugugua      660

acuguaaugg cacaagauaa accaacuguu gauaucgaac ucguaacaac aacagugagc      720

aauauggcgg aagugagauc auacuguuau gaggcaucaa ucucagacau ggcaucagau      780

ucaagauguc caacucaagg ugaagcguau cuggacaaac aaucagacac acaauaugua      840

uguaaaagga cauuaguuga uagaggaugg gguaauggau gcggacuauu cggaaaaggg      900

agcuuaguaa ccugugcuaa auuuacaugu aguaaaaaga ugacaggaaa aucaauacaa      960

ccagaaaauu uagaauacag aauaaugcua ucaguccaug gcagucaaca cagcggaaug     1020

auugguuaug aaacggacga ggauagagcu aaaguugaag uaacgccuaa uaguccuaga     1080

gcagaagcua cacuaggagg auuuggauca cuaggauuag acugcgaacc acgaacagga     1140

uuagauuuua gugauuuaua uuaccuaacg augaauaaca aacacugguu aguacacaaa     1200

gaaugguuuc acgauauacc auuaccaugg caugcaggcg cugauacagg aacaccacau     1260

uggaacaaua aagaggcuuu gguagaauuu aaagacgcac augcuaaaag gcagacagua     1320

guaguauuag gaucacaaga aggagcugug cauacagcac uugcaggagc acuagaagca     1380

gaaauggacg gggcaaaagg acgauuguuc ucaggacauu uaaaguguag acuaaaaaug     1440

gauaaacuaa gacuaaaagg uguaucauau agccuaugua cugccgcauu uacguuuacu     1500

aaagucccag cagaaacucu acauggaacc guaacugucg aagugcaaua ugcagguaca     1560

gauggcccuu guaaaauacc aguucaaaug gcaguggaca ugcaaacauu aacaccggua     1620

ggaagauuaa uaacagcaaa uccgguuaua acggaaucaa cagaaaauag uaaaaugaug     1680

cuagaauugg auccaccauu uggugauuca uauauaguaa uaggcguagg agacaagaaa     1740

auaacacauc acuggcauag aagcggauca acaauaggua aagcauuuga agcuacugua     1800

agaggagcaa aaagaauggc aguccuaggu gauacagcuu gggacuuugg uagcguagga     1860

ggaguguuua auucacucgg aaaagguauu caccaaaucu uuggagccgc uuuuaaauca     1920

uuauucggag guaugagcug guucucacaa auccuaauag gaacacuauu agugugguug     1980

ggacuaaaua cuaaaaaugg uagcauauca cuaacauguu uagcauuagg agggguuaug     2040

auauuucuaa guacagcugu aagcgcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10248
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10248
gggagaaagc uuaccaugga uuggacaugg auauuauuuu uaguagcagc agcaacgaga       60

guacauagug uaacaagacg agguuccgca uacuacaugu auuuagauag aaaugaugcg      120

ggagaagcca uaucauuccc gacgacauua ggaaugaaua aauguuauau ccaaauaaug      180

gauuuaggac acauguguga ugcaacaaug ucauaugaau guccaauguu agacgaagga      240

guugaaccug augauguaga uuguuggugu aacacaacua gcacgugggu ugucuaugga      300

acaugucauc auaagaaagg agaagcaaga cggaguagga gagcaguaac auugccauca      360

cacaguacaa gaaaacuaca aacacgaagu caaacauggu uagagagccg agaauauaca      420

aaacaucuaa uaagaguaga aaauuggauu uucagaaauc cagguuuugc auuagcagca      480

gcagcaauag ccugguuauu aggaucauca acaagucaaa aaguaauaua uuuagucaug      540

aucuuauuaa uagcaccagc guauucaaua cgauguauug gaguaucuaa cagggauuuc      600

guagaaggua ugucaggagg aacaugggua gauguaguac uugagcaugg aggaugugug      660

acaguaaugg cacaagauaa accaacgguu gauauagaau uaguaacaac aacaguauca      720

aacauggcag aaguaagaag uuauugcuau gaagcaagca uaucagauau ggcuucugac      780

agucgauguc caacacaagg agaagcauau uuagacaaac aaucugauac ucaauauguc      840

ugcaaaagaa cauugguaga uagggggugg ggaaauggau guggauuguu uggaaaagga      900

agucuaguaa cgugugcaaa auuugcuugc ucgaaaaaaa ugacuggaaa aaguauacaa      960

ccagaaaauc uugaauauag aauaauguua aguguucaug gaagucaaca uucaggaaug     1020

auuguaaaug auacuggaca ugaaacggac gaaaauaggg ccaaaguaga aauaacaccu     1080

aauaguccua gagcugaagc cacauuagga ggauucggau cauuaggacu cgacugugaa     1140

ccaaggacag gacuagauuu cucugacuug uauuacuuaa caaugaauaa uaaacauugg     1200

uuaguacaca aagaaugguu ucacgauaua ccacuaccau ggcaugcggg agcugauaca     1260

gguacuccac acuggaauaa uaaagaagca cucguagaau uuaaagacgc acacgcaaaa     1320

agacaaacag uuguaguauu agguucacaa gagggcgcag uacauacagc auuagcaggu     1380

gcuuuagaag cugaaaugga cggagcaaaa ggaagauuau caaguggaca ccuaaaaugc     1440

agauugaaaa uggauaaauu acgauuaaaa ggaguaucuu auagccuuug uacugccgca     1500

uuuaccuuua caaaaauacc agcagaaaca uuacauggaa cuguaacagu agaaguacaa     1560

uacgcuggca cugacggacc auguaaagua ccggcucaaa uggcgguaga uaugcaaaca     1620

uuaacaccag uaggucgccu aauaacagca aauccaguaa uaacugaaag cacugagaau     1680

uccaaaauga uguuagaauu ggauccaccu uuuggagauu cauacauagu aauuggagua     1740

ggugaaaaaa aaauaacaca ucacuggcau agauccggau caacaauagg aaaagcauuu     1800

gaagcaacag uucguggggc uaaaagaaug gcaguacuag gagauacagc cugggauuuc     1860

ggaucgguag gaggugcauu aaacucauua ggaaaaggua uacaucaaau auuuggagca     1920

gccuucaaaa gucucuuugg aggcaugagu ugguucaguc aaauauugau aggaacucua     1980

uuaauguggc ucggcuuaaa uacaaaaaau gguucaauuu cauuaaugug uuuagcauua     2040

ggaggaguau uaauuuucuu aucaaccgca guauccgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10249
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10249
gggagaaagc uuaccauggg aaaaagauca gcuggaagua uaaugugguu agcaucauua       60

gcuguaguaa uagcaugcgc aggagcugua acaagaagag gaagcgcaua uuauauguac      120

cuagauagaa acgacgcagg agaagcuauc ucuuuuccaa caacauuagg aaugaauaaa      180

uguuauauac aaauuaugga uuuaggacau augugcgacg caacaauguc auaugaaugu      240

ccuauguuag acgaaggagu agaaccagac gauguagauu guugguguaa uacaacaagu      300

acuuggguag uuuauggcac augccaucau aagaaagggg aagcucgaag aagcagacga      360

gcaguaacau uaccaaguca uagcacaaga aaacuacaaa caagaagcca aacaugguua      420

gaaucuagag aauauacuaa acauuuaaua cgcguagaaa acuggauauu cagaaaucca      480

ggauuugcac uagcagcagc agcaauagca ugguuauuag gaucaaguac aucacaaaaa      540

guaauauacu uaguaaugau acuauuaaua gcaccagcuu acucaauaag augcauagga      600

guaucaaauc gugacuuugu agaaggcaug agcgggggca cauggguaga uauuguacua      660

gaacauggug guuguguuac aguuauggcu caagauaaac cuaccguuga uauagaguua      720

guaacaacaa caguaagcaa uauggcagaa guaagaucau acuguuacga agcaucaaua      780

agcgauaugg caucagauag uagaugccca acucaaggag aagcauacuu agauaaacaa      840

ucagauacac aauauguaug caagcgaacc cuaguggacc gaggaugggg aaauggaugu      900

ggacuauuug gaaaaggcuc ucuuguuaca ugugcaaaau uugcauguuc uaagaaaaug      960

acaggaaaau caauacaacc ugaaaacuua gaauacagaa uaauguuaag cguucaugga     1020

agucaacacu cagguaugau aguaaaugau acaggacacg agaccgauga aaacagagca     1080

aaaguagaaa uaacacccaa cucaccaaga gcagaagcaa cccuuggcgg uuuuggaagu     1140

uuaggauuag auugugagcc uagaacuggu uuagauuucu cagauuuaua uuauuuaacu     1200

augaauaaua aacauuggcu aguccauaaa gaaugguuuc augauauacc uuugccaugg     1260

caugcgggug cagauaccgg aacaccacac uggaacaaca aagaagcacu uguagaauuu     1320

aaagaugccc acgcaaaaag acaaacugua guaguacuug gaagucaaga aggagcaguu     1380

cauacugcau uagcaggagc auuagaagca gaaauggaug gagccaaagg aagauuaagu     1440

ucuggacauc uuaaauguag guuaaaaaug gauaaauuaa gacuaaaagg cguuucauac     1500

ucauuaugua cagcagcauu caccuucaca aaaauaccag cugaaacauu acacggaaca     1560

guaacaguug aaguucaaua ugcaggaacu gaugggccau guaaaguacc ugcacaaaug     1620

gcaguagaua ugcaaacacu aacaccagua ggaagacuaa uaacagcaaa cccuguaauu     1680

acggaaucua cagaaaacag caaaaugaug uuagaguugg auccacccuu uggagauagc     1740

uauauaguua uaggaguggg ugaaaagaaa auaacgcauc auuggcacag aagugguagu     1800

acaauaggaa aagcauuuga ggcaaccgua agaggggcaa aaaggauggc aguccuagga     1860

gacacagcuu gggacuuugg aagcguaggc ggggcccuua auuccuuagg aaagggaaua     1920

caccaaauau uuggagcagc auuuaaauca uuauucggug gcaugucuug guuuucucaa     1980

auacuaauag gaacauuauu aauguggcuu ggacuaaaua ccaaaaaugg cucaaucuca     2040

uuaauguguc uugcuuuagg agguguacua auauuuuuaa guacagcagu aagugcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10250
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10250
gggagaaagc uuaccauggg uaaaagauca gcuggaucaa uaaugugguu agccucacua       60

gcaguuguua uagcaugugc uggagcggua acuagaagag guagugccua uuacauguac      120

cuagauagaa acgaugcagg agaagccaua ucauuuccaa caacauuagg uaugaauaaa      180

ugcuauauuc aaauaaugga uuuaggacac acaugugaug caacaauguc uuaugaaugu      240

ccaauguuag augaaggagu ugaaccagau gauguagauu guugguguaa uacaacaagu      300

acauggguug uauacggaac gugucaucau aaaaaaggag aagcacgaag aaguagacga      360

gcugucacau uaccaaguca uagcacucgu aaauuacaaa caagaaguca aacuuggcua      420

gaaagcagag aauauacgaa gcaucuaaua aggguugaaa acuggauauu cagaaaccca      480

ggauuugcuu uagcagcugc ugcaauagca uggcuauuag gaagcucaac aagucaaaaa      540

gucauauauu uaguaaugau acuucuaaua gcaccagcau acaguauuag augcauagga      600

guaaguaaua gggauuuugu agaaggaaug aguggaggua cauggguuga cguaguauua      660

gaacauggag gauguguaac aguaauggca caagacaaac cuacaguaga cauagaauua      720

guaacaacua caguuucuaa uauggccgaa guacgaagcu auuguuauga ggcaucaaua      780

ucagauaugg caucagacuc aagaugucca acucaagggg aagcguaucu agauaaacaa      840

uccgacaccc aauacguaug uaaacgaaca cuuguagaua gaggaugggg aaacgguugu      900

ggauuauuug guaaagguag uuuaguaacu ugcgcaaaau uugcauguuc aaaaaagaug      960

acaggcaaau caauacaacc agaaaaccug gaauauagaa uaauguuauc aguacauggc     1020

ucacaacaua gugguaugau cguuaacgau acuggacaug aaacagauga aaaucgagcg     1080

aaaguagaaa uaacgccaaa uucaccuaga gcugaagcaa cauuaggagg auuugggagc     1140

uuaggacuug acugugaacc uagaacagga uuagauuuca gcgaucuaua cuaucuuacg     1200

augaauaaua aacauuggcu cguacacaaa gagugguuuc augauauucc ucuuccaugg     1260

cacgcaggag cugauacugg gacaccacau uggaauaaua aagaagcuuu aguagaauuc     1320

aaagaugcac acgcaaaaag acaaacagua guuguguuag gaucucaaga aggagcggua     1380

cauacagcau uagcaggagc ccuagaagca gagauggaug gagcaaaagg acgguuauca     1440

ucaggacauc uaaaauguag acuuaaaaug gacaaauuaa gacuaaaagg aguaucuuau     1500

ucauuaugca cagcagcauu uaccuuuaca aaaauaccgg cagaaacacu ucauggaaca     1560

guuacaguag aaguacaaua cgcaggaaca gauggcccau gcaaaguccc agcacaaaug     1620

gcuguagaua ugcaaacauu aacuccagua gguagacuua uaacagcaaa uccgguaaua     1680

acagagucaa cggaaaauuc aaaaaugaug uuagaacuag auccaccauu uggagacagu     1740

uacauaguua uuggcgucgg agaaaaaaaa auaacacauc acuggcauag gagcggaagu     1800

acaauuggaa aagcauucga agcuacaguu agaggagcca aaagaauggc uguauuagga     1860

gacacugcgu gggacuuugg aaguguagga ggcgcauuaa auagcuuagg uaaaggaaua     1920

caucaaauau uuggugcggc auucaaauca cuguucggag gaaugaguug guuuucacaa     1980

auacuaaucg gaacuuuacu aaugugguua gguuuaaaug caaaaaaugg aucaauaagu     2040

uuaaugugcc uggcauuagg aggaguauug auauuuuuaa gcacagcagu gucagcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10251
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10251
gggagaaagc uuaccauggg aaaaagauca gcagggagua uaaugugguu agcaagucua       60

gcaguuguca uagcgugcgc aggugcaauc acuagaagag gaagcgcaua uuacauguau      120

uuagauagaa gugaugcagg aaaagcaauu ucauuugcua caacauuagg cgucaauaaa      180

ugucauguac aaaucaugga uuuaggucau augugugaug caaccaugag cuacgaaugu      240

ccaauguuag augaaggagu agaaccugau gacguagacu gcugguguaa uacaacaagu      300

acauggguag uauauggaac gugccaucac aaaaaaggag aagcaagaag aagccgaaga      360

gcggucaccc uaccaaguca uagcacuaga aaauuacaaa caaggaguca aacauggcua      420

gaaagcagag aauauacaaa acacuuaaua aaaguagaaa auuggauauu ucguaaucca      480

ggcuucgcac uaguagcagu agccauagca ugguugcuug gaucaucgac aagucaaaaa      540

guuaucuauu uaguaaugau auuauuaaua gcgccugcau acucaauacg auguauagga      600

guaucuaaca gagauuucgu agaaggaaug ucuggaggua cuuggguaga uguaguucug      660

gaacauggug gauguguuac uguaauggcc caagacaaac caaccguaga uauugaacua      720

guaacuacua cuguaagcaa cauggcggaa guacgaucau auuguuacga agcaaguaua      780

agugauaugg caucagauuc uagaugcccg acccagggag aagcauauuu agauaagcaa      840

agugacaccc aauacguaug uaaaagaaca uugguagaua gaggaugggg aaacggaugu      900

ggauuauucg gcaaaggaag uuuaguaaca ugugcuaaau uuaccugcag uaaaaaaaug      960

acaggcaaaa guauacaacc agaaaauuua gaauacagaa uaaugcuaag cguacaugga     1020

ucucaacauu caggaaugau aggauaugaa acagaugaag auagggcuaa aguagaaguc     1080

acaccuaaua gcccaagagc ggaagcgacc cugggugggu uuggaucauu aggauuagau     1140

ugcgaaccga gaaccggauu agauuuuagu gauuuauauu auuuaacaau gaacaauaaa     1200

cauugguuag ugcauaaaga gugguuccau gacauaccac uaccauggca ugcaggugca     1260

gacacuggaa caccacauug gaauaauaaa gaagcauuag ucgaauucaa agaugcucau     1320

gcgaaaagac aaacaguagu gguucuagga ucucaagaag gagcaguaca cacagcauua     1380

gcaggagcgc uagaagcaga aauggauggc gcaaaaggaa gauuauucag cggucauuua     1440

aaauguagau uaaaaaugga uaaauuaaga cuuaaaggag uaucauauuc ucuaugcaca     1500

gcagcauuua cuuuuacuaa aguaccagcc gaaacauuac auggcacggu aacugucgag     1560

guacaauaug cagguacaga cggaccuugu aaaauaccag uacaaauggc aguggauaug     1620

caaacauuaa cuccaguagg caggcuaaua acagcuaauc caguaauaac agaaucaacu     1680

gaaaacagca aaaugauguu ggaauuagau ccaccauuug gcgauuccua uauaguaaua     1740

gggguaggag auaaaaaaau aacacaccau uggcacagga guggaaguac aauagggaaa     1800

gcauuugaag cgacaguaag aggagcaaaa aggauggcag uacuaggaga uacagcaugg     1860

gauuuuggua gcgugggagg uguauuuaac ucacuaggca aggggauaca ucaaauauuu     1920

ggcgcagcau uuaaaagcuu auuuggagga augaguuggu uuucacaaau ucugauagga     1980

acucuuuugg uaugguuagg auuaaauaca aaaaacgguu cuauaaguuu aacaugucua     2040

gcauuagggg guguaaugau auuucuaagu acugcaguaa gcgcaugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10252
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10252
gggagaaagc uuaccauggg aaaaagauca gcagguucaa uaaugugguu agcaucauua       60

gcgguaguaa uagcaugugc aggagccgua acgcguagag guucagcuua uuauauguau      120

cuugauagaa acgaugcugg ugaagcaaua ucauuuccaa ccaccuuagg aaugaauaaa      180

uguuauauac aaauaaugga ccuaggacau augugugacg cgacaauguc auacgagugc      240

ccuauguuag acgaaggagu agaaccagau gacguagauu guugguguaa uacaacguca      300

acguggguag uauauggcac augccaccau aagaaaggug aagcucgcag aagcaggaga      360

gcaguuacau ugccaagcca uucgacuaga aaauugcaaa ccagaaguca aacaugguua      420

gaaaguagag aauauacuaa acaucugaua cgaguagaga acuggauauu uagaaaucca      480

ggauucgcgu uagcagcggc agcaauagcc ugguuauuag gaaguucaac aagucagaaa      540

guaauauauc uaguaaugau acuauuaauu gcuccugcau auucaauaag auguauagga      600

guaucaaaua gagauuuugu agaaggaaug ucuggcggaa cuuggguaga uguaguacua      660

gaacacgggg guugugucac uguaauggca caagauaaac caacagugga uauagaauua      720

guaaccacaa caguaaguaa uauggcagaa guaagaucau auuguuauga agcaaguaua      780

agugacaugg caucagauag cagaugucca acacaaggcg aagcauacuu agauaaacaa      840

uccgacacac aauacguaug uaaaagaaca uuagucgaua gaggaugggg aaauggaugu      900

ggauuauuug gaaaaggguc auugguaaca ugugcaaaau ucgcauguag caagaaaaug      960

acuggaaaau caauacaacc agaaaauuug gaauaucgaa uaauguuaag uguacauggc     1020

ucucaacauu cagggaugau uguaaacgac acuggacaug aaacagauga aaacagagca     1080

aaaguagaaa uaaccccgaa uaguccuaga gcagaagcaa cacuaggugg auuuggguca     1140

uuagguuugg auugugaacc aagaacaggc uuagauuuua gugaccuaua uuaccugacu     1200

augaauaaua aacauuggcu aguacauaaa gaaugguuuc augauauacc auuaccaugg     1260

cacgcaggag cugauaccgg aacuccacau uggaauaaca aagaagcauu gguugaauuu     1320

aaagaugcuc acgcaaaaag acaaaccgua guaguacuag gcucacaaga aggagcgguc     1380

cauacagcac uagcaggagc acuagaagca gaaauggacg gggcaaaagg aagguuauca     1440

aguggacauu uaaaguguag acuaaaaaug gacaaauuac gauuaaaagg aguaucauau     1500

ucauuaugua cagcagcauu uacuuuuacu aagaucccag cagaaacauu gcacggaaca     1560

guaacuguag aaguucaaua ugcaggaacu gauggaccau gcaaaguacc ugcucaaaug     1620

gcuguugaca ugcaaaccuu aacccccgua ggacgauuaa uaacagcaaa uccgguuaua     1680

acagaaucaa cugaaaauag uaaaaugaug uuagaacuag auccaccauu uggugauagu     1740

uacauaguua uaggaguagg agaaaagaaa auaacacauc auuggcauag gagcggauca     1800

acaauaggga aagcauuuga agcaacagua agaggagcca aaagaauggc aguauuagga     1860

gauacggcgu gggauuuugg aagcguugga ggcgcauuaa auucccuagg aaaaggaaua     1920

caucaaauau ucggagcagc auuuaaauca uuauuuggug gaaugucuug guuuagucaa     1980

auacuaauag gaacauuauu aaugugguua ggauuaaaua cuaaaaacgg aucaauaagc     2040

cugaugugcu uagcacuagg uggaguacua aucuuuuuga guacagcagu gucagccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10253
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10253
gggagaaagc uuaccauggc aauaagcgga guaccaguau uaggauuuuu uauaauagca       60

guuuuaaugu ccgcucaaga gagcugggcc guuacuagaa gaggaagugc auauuauaug      120

uaucucgaua gaaaugacgc aggagaagcg auaucauuuc caacaacauu aggaaugaau      180

aaaugcuaua uacaaauuau ggaucuagga cauaugugug augcaacaau gaguuacgaa      240

uguccaaugu uagaugaagg cguagaaccu gaugauguag acuguuggug caacacaacu      300

ucaacauggg uaguauacgg aacgugucau cacaaaaaag gggaagcacg aagaucaaga      360

agggcaguaa ccuuaccauc acacucaacu agaaaauuac aaacuagaag ucaaacaugg      420

cuagaaucaa gagaauauac aaaacauuua auucgaguag aaaacuggau auucagaaau      480

ccaggauuug cacuagcagc agcggcaaua gcaugguuac uaggaucauc aacuucacaa      540

aagguaaucu aucuaguuau gauacuucuc auagcaccag cuuacaguau uagaugcauu      600

gguguaucua aucgagauuu uguugaagga auguccggag gaacgugggu agacauaguu      660

uuggaacaug gaggaugugu aacggugaug gcacaagaca aaccaacagu agauauugaa      720

uuagugacaa caacaguuuc aaauauggcu gaagucagaa gcuauuguua ugaagcaagu      780

auaagugaua uggccaguga uucaagaugc ccuacacagg gggaagcaua ucuugauaaa      840

caaucugaua cucaauaugu cuguaaaaga acuuuaguug auagaggaug gggcaaugga      900

uguggcuuau ucggaaaagg aaguuuagua acaugugcua aauucgcaug uucaaaaaaa      960

augacaggaa aaagcauaca accugaaaau cuagaauauc guauaauguu aaguguacac     1020

ggaucacaac acucaggaau gauaguuaau gauacuggac augaaacaga ugaaaacaga     1080

gcaaaaguag agauuacacc aaauaguccg cgcgcugaag caacauuggg aggauuugga     1140

agccuagguu uagacuguga accaagaacc ggucuagauu uuucagaccu auacuauuug     1200

acaaugaaua acaaacauug gcuaguucau aaagaauggu uucaugauau accacuaccc     1260

uggcacgcag gagcagauac aggaacaccu cauuggaaua auaaagaagc cuuaguagaa     1320

uucaaagaug cacaugccaa aagacaaaca guaguaguau uaggaucaca agaaggagcu     1380

guucauacag cacucgcagg cgcauuagaa gcugaaaugg auggagcuaa aggacgacua     1440

ucuucuggac acuuaaaaug ucgguuaaaa auggauaaau uacgauuaaa aggaguauca     1500

uauagccuau guacugcggc auuuaccuuc acaaaaauac cagcagaaac acuacaugga     1560

acagucacgg uagaaguaca guaugcagga acagauggac cauguaaagu accagcucaa     1620

auggcaguug acaugcaaac acuaacacca guaggaagac uaauaacugc aaauccugua     1680

aucaccgaaa guacugaaaa caguaaaaug augcuugaau uagauccacc guuuggagau     1740

agcuauauag uaauaggagu uggagaaaaa aaaauaaccc accauuggca caggagugga     1800

ucaacauuag gaaaagcuuu uucuacaaca uugaaaggag cgcaacgucu agcugcauua     1860

ggagacacag cuugggauuu uggaucaaua ggaggaguau uuaauucaau aggaagagcc     1920

gugcaccaag uauucggagg agccuuuaga accuuauucg gcggaaugag uuggauaaca     1980

caaggacuaa ugggagcucu auuacuaugg augggaguua augccagaga uagauccauu     2040

gcacuggccu uuuuagcgac aggaggggua uuaguauuuu uagcaacaaa uguacaugca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10254
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10254
gggagaaagc uuaccauggc aauaagcgga guaccaguau uaggauucuu uauaauagca       60

guacuaaugu cagcucaaga aaguugggcc guaacaagaa gaggauccgc auauuauaug      120

uaucuagaua gaaaugaugc uggugaagca auaucuuucc cuaccacauu aggaaugaau      180

aaauguuaua uacaaauuau ggaucuggga cacacaugug augcaacgau gucuuacgag      240

uguccaaugu uagacgaagg uguugaacca gacgauguug auuguuggug caacaccaca      300

ucaacuuggg uaguauaugg uacaugucau cacaaaaaag gcgaagcgag aagaucaagg      360

agagccguaa cauuaccaag ucacucaacu aggaaauuac aaacaagauc acaaaccugg      420

uuagaaucaa gagaauauac aaaacauuua auaagaguag aaaauuggau cuuucgaaac      480

ccaggauuug cuuuagcugc agcagcaaua gcuugguuau uaggaucaag cacaucacaa      540

aaaguaauau auuuaguaau gauauuacua auagcuccag cauacuccau cagauguauu      600

ggugucucca auagagauuu cguugaagga augagcggag gaacuugggu agauguaguu      660

uuagaacaug gcggaugugu aacuguaaug gcacaagaua aaccaacagu agacauagag      720

uuaguuacaa caaccguauc caauauggcu gaaguaagaa gcuauugcua ugaagcuuca      780

auaucugaua uggcaaguga cucaagaugc ccaacacaag gugaagcaua ucuagacaaa      840

caaucagaca cacaauaugu auguaaaagg acacuaguag acagaggaug gggaaauggu      900

uguggcuuau uugggaaagg gaguuuaguc acaugcgcaa aauucgcaug uucaaaaaaa      960

augacaggaa aaucaaucca accagaaaau cuagaauauc guauaaugcu aucuguucau     1020

ggaucucaac auagcggaau gauuguaaau gacacaggac acgaaaccga ugaaaacaga     1080

gcaaaaguag aaauaacacc aaauucccca agagccgaag caacacuagg agguuuugga     1140

ucuuuaggac ucgauuguga accaagaaca ggauuggauu ucagcgaccu uuauuacuua     1200

acaaugaaua auaaacacug guuaguacau aaagaguggu uccaugauau uccauuaccu     1260

uggcacgcag gagcugauac aggaacucca cacuggaaua auaaagaagc auuaguagaa     1320

uucaaagaug cacacgcaaa acgacaaacu guaguaguau ugggaucaca agaaggagca     1380

gugcauaccg cauuagcugg agcacuagag gcagaaaugg auggagcaaa aggccgauua     1440

ucaucaggac aucuuaaaug ucgucuaaaa auggauaaau ugagacuaaa aggcguauca     1500

uauucacuuu guaccgcugc uuuuacuuuu accaaaauac cagccgaaac ucuacauggu     1560

accguaacag ucgaaguuca auaugcaggg acagauggac cauguaaagu uccagcacaa     1620

auggcagucg acaugcaaac auuaacgccg guaggaaggu ugauuacagc aaauccagua     1680

auuacagaaa gcacagaaaa uucaaaaaug auguuagaau uagauccacc cuuuggagau     1740

aguuauauag uaauaggagu aggagaaaaa aaaauuacac accacuggca ccggucagga     1800

ucuacucuag gaaaagcuuu uaguacuaca uuaaaaggag cacaaagauu agcagcacua     1860

ggagauacag ccugggauuu uggaucuaua gguggagugu uuaacuccau aggaagagcu     1920

guacaccaag uuuuuggcgg ugcuuuuaga acucuuuuug gugguauguc auggauaaca     1980

cagggacuua ugggggcuuu acuacugugg augggaguaa acgccagaga uagaucuaua     2040

gcacuagccu uucuagcaac aggaggagua uuagucuucu uagcaacuaa uguacacgca     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10255
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10255
gggagaaagc uuaccauggc cauaagugga guaccuguau ugggauucuu uauaauagcg       60

guauuaaugu cagcacaaga aucaugggcg auaacaagaa ggggaagcgc cuauuauaug      120

uacuuagaua gaagcgaugc aggaaaagca auaaguuuug caacuacucu aggaguaaau      180

aaaugccaug uucaaauaau ggauuuagga cacaugugug augcuacaau gucuuaugaa      240

uguccgaugu uagacgaagg agucgaaccu gacgaugugg auugcuggug uaacacaacu      300

ucaacauggg uaguauacgg aacuugucau cauaaaaaag gugaagcgcg aagaucccga      360

agagcaguaa cucucccuag ucacucaaca cgaaaguuac aaacaagaag ucaaacuugg      420

uuagagagua gagaauauac aaagcacuua auaaaaguag aaaauuggau uuuuaggaau      480

ccagguuuug cgcuuguagc aguagcaauc gcuugguuau uaggaucauc aacaucacaa      540

aaaguaauau acuuaguaau gauauuacuc auagcaccag cauacaguau aagauguauu      600

gggguaagua aucgagauuu uguggaagga augaguggug gaacaugggu ggauguagua      660

cuugagcacg gaggcugcgu aacuguaaug gcucaagaua aaccaacagu agauaucgaa      720

uuaguaacua cuacaguuuc aaacauggcu gaaguaagau cguauuguua ugaagcgagc      780

auaucagaua uggccaguga cucaagaugc cccacgcaag gagaagcaua ucuugauaaa      840

caaagugaua cucaauaugu auguaaaaga acauuaguag accgaggaug gggaaauggc      900

uguggauuau ucgguaaagg aaguuuagua acaugcgcaa aauuuacaug uucaaaaaaa      960

augacaggaa aaagcauuca accggaaaac cuagaauauc gaauuauguu gucgguucac     1020

ggaagucagc auagcggaau gauaggauau gaaacagaug aagauagagc aaaaguagaa     1080

guaacaccaa auaguccacg ugcagaagca acucucggcg gguuuggaag ucuaggacua     1140

gauugugaac caagaacagg acuagauuuu agugaucuau acuauuuaac aaugaauaau     1200

aaacauuggu uaguccauaa agaaugguuu caugauauac cacuuccaug gcaugcugga     1260

gcagauacag gcacaccaca uuggaauaau aaagaagcuc uaguagaguu uaaagacgcc     1320

caugcaaaaa gacagacagu gguuguacua gguagccaag aaggagcugu ucauacagca     1380

uuagcaggag cccuugaagc agaaauggau ggagcuaaag gaagauuauu uaguggacau     1440

uuaaaaugca gacucaaaau ggauaaacuu agacuaaaag guguaucuua cagucuaugc     1500

acagccgcau uuacauuuac aaaaguccca gcagagacac uacacggaac agugacagua     1560

gaaguacaau augcaggcac ugauggacca ugcaaaauac ccguacaaau ggcaguagau     1620

augcaaacgu uaaccccagu aggaagacua aucacugcaa acccaguaau uacagaaagu     1680

acugagaauu caaaaaugau gcuagaauua gauccaccau ucggagacag uuacauagug     1740

auaggaguag gagauaaaaa aauuacacau cauuggcaua gaucagguag uacauuagga     1800

aaagcauuua gcacaacauu aaaaggagca caaagauuag cagcauuagg cgauacagca     1860

ugggauuuug ggagcauagg uggcguauuc aacucuauag gcagggccgu gcaucaagua     1920

uuugguggag cauucagaac ucuauuugga ggcaugucau ggauuacaca agguuuaaug     1980

ggagcauuac uauuauggau gggaguaaau gcaagagaua gaaguauagc auuagcguuu     2040

uuagcaacag gaggaguuuu aguauucuua gcaacuaaug uccacgcaug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10256
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10256
gggagaaagc uuaccauggc aauuucagga guaccaguau uaggauucuu uauuauagcc       60

guacuaaugu cugcacaaga aagcugggcg gugacaagaa ggggaagcgc auacuauaug      120

uaucuagaca gaaaugaugc aggagaagca auaucuuuuc cuacaacauu aggaaugaau      180

aaauguuaua uacaaauaau ggacuuagga cauaugugug augcaacgau gagcuaugaa      240

ugcccgaugc ucgacgaagg uguagaacca gaugauguag auuguuggug uaauaccacu      300

agcacauggg uagucuaugg aacgugccau cauaaaaagg gcgaagcccg aagaucuaga      360

cgagcaguaa cguugccauc ucauaguaca agaaagcugc aaacaagaag ucaaacaugg      420

cuagaaucga gagaauauac aaaacacuua auuagaguag aaaauuggau auuuagaaau      480

ccaggauuug cuuuagcagc ugcagcaaua gccuggcuau uaggaucauc aacaagucaa      540

aaagugauau auuuaguaau gauacucuug auagcaccag ccuauucaau aagauguaua      600

gguguaucaa acagggauuu uguggaagga augaguggag gaacuugggu agacgucguc      660

uuagaacaug gaggaugugu aacaguuaug gcacaagaua aaccaacagu agauauagaa      720

uuagucacaa cuacuguauc uaauauggca gaaguaaggu cauacugcua ugaagcauca      780

auuagcgaua uggcuaguga uaguagaugc ccaacucaag gagaagcaua ucuagacaaa      840

cagucagaua cacaauaugu augcaaaaga acacuuguag auagagggug gggaaacgga      900

uguggacuuu uugggaaagg cagcuuagua acaugugcaa aauuugcaug caguaagaaa      960

augacuggaa aaaguauaca gccagaaaac cuagaauaca gaauaauguu aaguguacac     1020

ggaucucaac auaguggaau gauaguaaau gauaccggac acgaaacuga ugaaaaucga     1080

gcuaaaguag aaauaacccc aaacaguccg agagcagaag caacuuuagg aggauuugga     1140

aguuuaggac uagacuguga accaagaaca ggacuugacu uuucugaucu cuauuacuua     1200

acaaugaaua auaaacacug gcuaguacau aaagaauggu uucaugauau accacuacca     1260

uggcaugccg gugcagauac uggcacaccu cauuggaaua acaaagaggc uuuaguagaa     1320

uuuaaagaug cacaugcaaa aagacaaaca guaguagugc uaggaucaca agaaggugca     1380

guacacaccg cacuagccgg agcauuagaa gcugaaaugg auggugcaaa aggaaggcua     1440

ucaucaggac auuuaaaaug uagguuaaaa auggauaaac uaagauuaaa aggaguauca     1500

uauucauuau guacugcagc auucacauuu acaaaaauac cggccgaaac gcuacaugga     1560

acuguaacag uugaaguuca auaugcggga acagauggac cgugcaaagu accagcacaa     1620

auggcaguag auaugcaaac ucuaacacca guuggaagau ugauaacagc uaauccaguu     1680

auaacagaaa guacagaaaa caguaaaaug augcuagaau uagauccacc guuuggagau     1740

ucuuacauug uuauaggagu aggugagaag aaaauuacuc aucacuggca uagaaguggc     1800

ucaacacuag gaaaagcauu cagcacuaca cuuaaaggag cacaaagauu agcagcucua     1860

ggggauacag ccugggacuu cggcucaauc ggaggagucu ucaauaguau agggagagca     1920

guacaccaag uauuuggagg agcauuuaga acauuauuug gagguaugag cuggauaacu     1980

caagguuuaa ugggagcacu ucuccuaugg auggguguca acgcaagaga ccguaguaua     2040

gcuuuagcuu uucuagcaac aggaggagua uuaguauucu uagcaacaaa cguccaugcg     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10257
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10257
gggagaaagc uuaccaugga uuggacaugg auauuauuuu uaguagcagc ugcaacaaga       60

guacauagug uaacaaggcg cggaagcgcu uacuauaugu aucuggauag aaacgaugca      120

ggagaagcua uaucauuucc uaccacacuu ggcaugaaua aauguuauau ccaaauaaug      180

gaucuugguc auaugugcga cgcaaccaug aguuacgaau gcccaaugcu agaugaaggu      240

guagaaccug augaugucga uuguuggugc aacacgacuu cgacuugggu aguauauggu      300

acaugucauc acaaaaaagg agaagcuaga agaaguagaa gagcagucac auuaccauca      360

cauucaacaa ggaaauuaca gacaagaagu caaacauggc uagaaucaag agaauauacc      420

aaacauuuaa uaagaguuga aaauuggaua uucagaaauc caggauuugc auuagcagcu      480

gccgccauag caugguuauu agggaguuca acaucacaaa aaguaauuua cuuaguuaug      540

auucuuuuaa uagcaccugc auauagcauu agauguauug gaguaaguaa uagagauuuu      600

guggaaggaa ugucuggugg aacaugggug gauaucguac uggaacaugg aggaugugua      660

acuguaaugg cgcaagauaa accaacugua gacauagaau uaguuacaac uacaguauca      720

aauauggccg aaguuagaag uuauuguuau gaagcaucaa uaagugauau ggcaucagau      780

aguagguguc caacacaagg agaagcuuau uuggauaaac aauccgacac acaauacgua      840

ugcaaaagga cucuaguuga uagaggaugg ggaaauggau gcggauuauu cgguaaagga      900

ucauuaguua ccugugcuaa auucgcgugu ucuaaaaaaa ugacagggaa aaguauucaa      960

ccagaaaacc uagaauacag aauuaugcuu agugugcaug gaagccaaca uucaggaaug     1020

auugucaacg acacagguca ugaaacagau gaaaaucgag caaaaguaga aauaacaccu     1080

aauucaccac gugcagaagc aacauuagga ggauuuggau cauuaggauu agauugugaa     1140

ccaaggacag gauuagauuu cagcgacuua uacuaucuga ccaugaauaa uaaacacugg     1200

uuaguacaca aagaaugguu ccaugauaua ccauuaccau ggcacgcagg agcagacaca     1260

ggaacaccac acuggaauaa uaaagaggcc cuuguggaau ucaaagaugc acaugcaaaa     1320

cgacaaacag uaguaguacu uggaucacaa gagggagcag uucauacagc auuagcagga     1380

gcacuugagg cagaaaugga cggugcaaaa ggaagauuaa guaguggaca uuuaaaaugu     1440

agacuaaaaa uggacaaacu aagauuaaaa ggaguuagcu auaguuuaug uacagcagca     1500

uucacauuca caaaaauucc agcagaaaca uuacacggaa cagucacagu agaaguacaa     1560

uaugcaggaa cagacggacc auguaaaguu ccggcacaaa uggcaguaga uaugcaaaca     1620

uuaacaccag uuggacguuu aauaacagcu aauccaguaa uaacugaaag cacggaaaau     1680

ucuaaaauga ugcuagaacu cgauccaccu uuuggagauu cuuacaucgu aauaggagug     1740

ggggaaaaaa aaauuacuca ccacuggcau agaaguggau caacauuagg uaaagcauuc     1800

aguacaaccu uaaaaggagc acaacgacua gcagcauuag gcgacacagc augggauuuu     1860

gguucgauag gaggaguguu uaauucaaua ggacgcgcag uucaucaagu uuuuggagga     1920

gcauuuagaa cauuauuugg aggaauguca uggauaacac agggacuuau gggagcccua     1980

uuauuaugga ugggagugaa ugcuagagau agaucaauag cucuagcauu cuuagcaaca     2040

ggugguguau uaguguuucu agcaacaaau guacacgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10258
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10258
gggagaaagc uuaccaugga cuggacuugg auacucuuuu uaguagcugc agcaacaaga       60

guacacucag uaacacgaag gggauccgca uauuauaugu auuuagauag aaacgaugca      120

ggcgaagcaa uuucauuucc aacaacauua ggcaugaaua aaugcuauau ucaaauaaug      180

gaccuagggc auacaugcga ugccacuaug agcuaugaau guccaauguu agaugaagga      240

guggaaccag augauguaga cugcuggugu aauacaacaa guacaugggu uguauauggc      300

acaugccauc auaaaaaagg ggaagcaaga agauccagaa gagcuguaac auuaccuagc      360

cauaguacaa gaaaauuaca aacaagaagc caaacauggc uagaaucaag agaguauaca      420

aaacaccuaa uaagaguaga aaauuggaua uuuagaaacc cagguuucgc auuggcagca      480

gcagcaauug cgugguuacu aggaucauca acaucacaaa aaguaauaua ucuaguaaug      540

auauuauuaa uagcaccagc uuauucaaua agauguauag gcguaaguaa uagagauuuu      600

guagaaggaa ugagcggagg aacaugggug gauguaguau uagagcaugg aggaugugua      660

accguaaugg cacaagauaa accaacaguu gauauagagu ugguuacuac aacuguuuca      720

aauauggcag agguacguuc uuacugcuau gaagcaagua ucagcgauau ggcaagugac      780

ucacguugcc caacucaggg ggaggccuau uuagacaaac aauccgauac acaauauguu      840

uguaaaagaa cacuaguaga cagaggaugg ggaaacggau gugguuuauu uggaaaaggc      900

aguuuaguca cuugugcaaa guucgcaugu aguaaaaaaa ugacagguaa aagcauccaa      960

ccagagaacc uagaauacag aauaauguua aguguucacg gaucucaaca uagcggaaug     1020

auugugaacg auacugguca ugaaacggau gaaaauagag cuaaaguaga aauuacacca     1080

aauaguccca gagcagaagc aacccuaggc ggauucggau cauugggacu ugacugcgaa     1140

ccuagaaccg gacuugauuu uucugacuug uacuacuuaa caaugaauaa uaaacacugg     1200

uuaguucaua aagaaugguu ccaugauaua ccacugccau ggcacgcagg agcagauacu     1260

gguacaccuc auuggaauaa uaaagaagca uuaguugaau ucaaagacgc acaugcaaaa     1320

agacaaacag uaguaguauu aggaagccaa gaaggagcag uacacacagc acuagcagga     1380

gcuuuggaag cagaaaugga cggugcuaaa ggaagauuga gcaguggaca ucuaaaaugu     1440

cgacuuaaaa uggauaaauu aagauuaaaa gguguaagcu auucacuaug uacugcagca     1500

uucacauuua cgaaaauacc agcagaaacu cuacacggua caguuacagu agaaguccaa     1560

uaugcaggaa cagauggacc auguaaaguu cccgcacaaa uggcagugga caugcaaaca     1620

uuaacuccgg uuggaagauu aauaacugca aauccaguga uuacggaauc gacagaaaau     1680

ucaaaaauga ugcuugaauu agacccuccu uuuggagauu cauacauagu aaucggugua     1740

ggugaaaaaa aaauuacaca ucauuggcau cggucagguu caacuuuagg gaaagcauuu     1800

ucgacaacac uaaaaggcgc acaaagauua gcagcacuag gcgacacagc augggauuuc     1860

ggaaguauag gaggaguuuu uaauagcaua ggaagagccg uacaucaagu auuuggagga     1920

gcuuuucgga cucuauuugg aggcauguca uggauaacac aagguuuaau gggggcauua     1980

uuauuaugga ugggaguuaa ugcacgugau agaagcauag cguuagcauu uuuagcaacu     2040

ggaggaguau uaguauucuu agcaacaaac guacaugcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10259
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10259
gggagaaagc uuaccaugga cuggacaugg auacuauuuc uaguagccgc agcgacuaga       60

guacauucaa uuacaagaag aggaagcgca uauuauaugu auuuagauag gucagaugcu      120

gggaaagcaa uaucauucgc aacuacuuug gguguaaaca aaugccacgu acaaauaaug      180

gaucuaggcc auauguguga ugcaacaaug ucauaugaau guccaauguu agacgaaggu      240

guagaaccag augauguuga uugcuggugu aacacaaccu caaccugggu gguuuauggu      300

acuugucacc acaagaaagg ugaagcuaga aggaguagga gggccguaac acuuccauca      360

cauagcacaa gaaaacuuca aacuagauca caaacauggu uagaaucaag ggaauacaca      420

aaacaucuaa ucaaagucga aaauuggaua uuuagaaacc caggauuugc ucuaguugca      480

guagccauag ccuggcucuu aggaucaagc acuucacaga aaguuauaua uuuaguuaug      540

auacuauuaa uagcacccgc uuauuccaua agauguauag gagugaguaa ucgagacuuu      600

guagaaggaa ugucaggugg uacgugggug gauguaguau uagaacaugg aggaugugua      660

acaguuaugg cacaagacaa accaacagua gauauagaau uagucacaac aacaguauca      720

aauauggcag aaguuagauc auauugcuac gaagcgucaa uaagugauau ggccucagau      780

aguagauguc cuacacaagg agaagcguau uuagacaaac aaucagacac acaauauguu      840

uguaaaagga cucuaguuga uagaggaugg gguaauggau guggauuauu cggaaaagga      900

ucccuaguaa caugugccaa auuuacaugu aguaagaaaa ugaccggaaa aucuauacag      960

ccagaaaauu uagaauaucg uauaauguua agcguacaug guagucaaca cagcgguaug     1020

auaggguaug aaaccgacga agacagagca aaaguagaag uaacaccaaa cucaccaaga     1080

gcugaagcca ccuuaggagg auucggaagu cuuggauuag auugugaacc aagaacugga     1140

uuagauuucu cagaccuuua uuauuuaacu augaauaaua aacauugguu aguacauaaa     1200

gaaugguuuc acgacauacc uuuaccaugg caugcaggag cagauacagg aacgccacac     1260

uggaacaaua aagaagcauu aguagaauuu aaagaugcuc acgcuaaaag acaaacaguu     1320

guaguauuag ggucacaaga aggugcugua cauacugcau uagcaggagc acuagaagcc     1380

gaaauggacg gagcaaaagg cagauuauuu aguggacauc uuaaaugcag auuaaaaaug     1440

gauaaacuaa gacuaaaagg aguaagcuac agccuaugua cugccgcauu uacauuuaca     1500

aaaguuccag cugaaacacu acaugggaca guaacaguag aaguccaaua cgcaggaacu     1560

gauggaccau guaaaauacc aguacaaaug gcaguagaca ugcaaacacu uacaccaguu     1620

gggagguuaa uaacagcuaa ucccguaauc acugaaucaa cagaaaauag uaaaaugaug     1680

uuagaacuug acccaccauu uggagauuca uacauuguaa uuggaguagg ugauaagaaa     1740

auaacacauc auuggcauag aucuggauca acacuuggaa aagcauucag cacaacauug     1800

aaaggagcac aaagauuagc ggcauugggg gauacagcau gggauuucgg aaguauagga     1860

ggaguauuua auucaauugg ucgagcaguc caccaaguau uuggaggagc cuuuagaaca     1920

uuauucggag gaaugucaug gauuacacaa ggauuaaugg gagcccuauu gcuuuggaug     1980

ggaguuaaug cacgagaccg uucgauagcu cucgcauuuc ucgcuacugg cggaguucua     2040

guauuuuuag caacgaaugu ucaugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10260
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10260
gggagaaagc uuaccaugga uuggacaugg auucuauuuc uuguagcagc cgcaacaagg       60

guacauagug ucacaagaag gggaagugca uauuauaugu aucuagacag aaaugaugcu      120

ggugaagcca uaaguuuucc aacaacgcua ggaaugaaua aauguuacau ccaaauaaug      180

gaucucggcc auauguguga ugcgacaaug aguuaugaau gcccaaugcu cgaugaaggg      240

guagaaccag augauguaga uuguuggugu aauacaacau caacuugggu aguuuaugga      300

acaugucauc auaaaaaagg ugaagcuaga agaucgagga gagcuguaac uuuaccuucu      360

cauagcacaa gaaaauuaca aacaagaagc caaacuuggu uagaaagcag agaauacaca      420

aaacauuuaa uuagaguaga aaauuggaua uuucgaaauc caggauucgc auuagcugca      480

gcagcaauag cauggcuucu aggcuccagu acaucucaaa aagugaucua uuuaguaaug      540

auacuucuaa ucgcaccagc auacaguauc agaugcaucg guguaucaaa uagagauuuu      600

guugaaggaa ugucgggcgg aacaugggua gacgucguac ucgagcaugg cggcugugua      660

acaguaaugg cacaagacaa accaacaguu gauauagaau uaguuacaac uacgguaagu      720

aauauggcag aaguuaggag cuacuguuau gaagcaucaa uaucagauau ggcaucagau      780

ucaaggugcc caacgcaagg agaagcauau uuagauaaac aaagugacac ucaauacgua      840

uguaaaagaa cauuaguuga uagaggaugg ggaaacggau gugguuuauu uggaaaagga      900

agcuuaguaa caugugcaaa auuugccugu ucaaaaaaaa ugacaggaaa aaguauacaa      960

ccagaaaauc uagaauaccg uauaaugcua ucaguacaug gaagucaaca cagcgggaug     1020

auaguaaacg acacaggaca cgaaacagau gaaaaucgug caaaggucga aauuacccca     1080

aacagcccaa gagcagaagc aacucuugga gguuucggau cauuaggacu agauugcgag     1140

ccaagaaccg guuuagauuu uagcgauuua uacuauuuaa caaugaauaa uaaacauugg     1200

uuaguucaua aagaaugguu ucaugauaua ccacuaccau ggcaugcagg ugcggauacu     1260

ggaacaccuc auuggaacaa uaaagaagca cuaguagaau ucaaagacgc acaugcuaaa     1320

agacaaacag uugucguauu aggaagucaa gaaggagccg uacauacugc acuagcaggu     1380

gcguuagaag ccgagaugga uggagcaaaa ggccgacuua guagcgguca uuuaaaaugc     1440

agauuaaaaa uggauaaauu gagacugaaa ggugugucau auucacuaug uacugcagca     1500

uuuacauuca caaaaauacc ugcagaaaca cuacacggaa cugucacagu agaaguacaa     1560

uacgcaggua cagauggucc auguaaagua ccagcucaaa uggcuguaga caugcaaaca     1620

uuaacaccag uuggaagacu aauaacagca aaucccguaa uaacugaaag cacagaaaau     1680

ucaaaaauga uguuggaacu ugauccacca uuuggcgaua guuacauagu aauaggaguu     1740

ggagaaaaaa aaauaacaca ucauuggcac agaaguggaa gcaccuuagg aaaagcuuuu     1800

ucaacaacau uaaaaggagc acaaagauua gcagcauuag gugacacagc augggauuuu     1860

ggcucaauug gugguguauu caauaguaua gggagagcag uacaucaagu uuucggcgga     1920

gcauuuagaa ccuuauuugg cggaaugagu uggaucacuc aaggacuaau gggagcacua     1980

uuacucugga uggguguaaa ugcaagagau agaagcauag ccuuagcauu ucuugcuaca     2040

ggaggaguau uaguauuccu agcuaccaau guacaugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10261
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10261
gggagaaagc uuaccauggg caaacguagu gcaggaucga uaauguggcu agccucauua       60

gcaguaguaa uagcaugugc cggagcagua acgaggcgcg gaucagcuua uuauauguac      120

cuagacagaa augaugcagg agaagcaaua ucauucccua caacacuugg aaugaauaaa      180

uguuauauac aaauaaugga uuuaggacau augugcgacg caacuaugag uuacgaaugc      240

ccaaugcuug augaaggcgu agaaccagau gauguugauu gcuggugcaa cacaacguca      300

accuggguag uguaugggac augucaucac aagaaaggag aagcuagaag aaguagacga      360

gcaguaacgu uaccgaguca uaguacgaga aaauuacaaa cacgaucaca aacaugguua      420

gaaagcagag aguacacaaa acauuuaauc agagucgaaa auuggauuuu caggaauccu      480

ggguuugcau uagcagcagc ggcaauagca ugguuauuag gaucaaguac aucacaaaaa      540

guaaucuauc uaguaaugau ucuguuaauc gcucccgcau auuccauuag auguauagga      600

guaucaaaca gagauuuugu ggaaggaaug uccggaggaa cauggguaga uauaguuuug      660

gaacauggag gguguguaac agucauggca caggauaaac caacggugga uauagagcua      720

guaacaacaa caguaucaaa uauggcagaa guaagaucuu auuguuauga agcaucuaua      780

agugauaugg ccagugacag uagaugucca acacaagggg aagcauaucu agacaaacaa      840

agugauacac aauacguaug uaaaagaaca cuuguagaua gaggaugggg aaauggaugc      900

ggauuauucg gaaaaggauc uuuaguaaca ugcgcaaaau ucgcaugcuc aaagaaaaug      960

acagguaaaa guauucaacc agaaaaucuc gaauauagaa uuauguuaag uguucaugga     1020

ucacaacaca gugguaugau aguaaacgau acagggcaug aaaccgauga aaacagagca     1080

aaaguagaaa uaacaccuaa uucaccuaga gcagaagcua cucugggagg auucgggagu     1140

cuuggacuag auugcgaacc aagaacaggu cuugauuuuu cugaucuaua uuaucucaca     1200

augaauaaua aacauugguu aguucacaaa gaaugguuuc acgacauacc guuaccaugg     1260

caugccggag cagauacggg aacuccacau uggaauaaua aagaagcucu uguagaauuu     1320

aaagaugcuc augcaaaaag gcaaacagua guaguuuuag gcagccaaga aggagcagua     1380

cacacagcuu uagcuggagc auuagaagca gaaauggaug gagcgaaagg aagacuaucg     1440

ucaggucauu ugaaaugcag acuuaaaaug gacaaauuaa gacuaaaagg aguuucauau     1500

ucauuaugua cugcagcauu cacuuuuaca aaaauaccag cagaaacacu acauggcaca     1560

guaacuguag aaguacaaua ugcuggaaca gacggaccau gcaaaguccc ugcccaaaug     1620

gcaguggaua ugcaaacacu aacaccagug ggaagacuaa uaacagcaaa uccaguaaua     1680

acagaaucaa cagaaaacuc aaaaaugaug uuggaauuag auccaccuuu uggagacagu     1740

uauauuguaa uaggcguagg agaaaagaaa auuacacacc auuggcauag aaguggaagc     1800

acacuuggaa aagccuuuag cacaacauua aaaggagcuc aaagauuagc ugcacuagga     1860

gauacagcgu gggauuuugg aaguauagga ggugucuuua auaguaucgg cagagcagua     1920

caucaaguau ucggaggagc auucagaaca cuauuuggcg gcaugucaug gauuacacaa     1980

gguuuaaugg gagcucuauu auuauggaug gguguaaacg cuagggaucg gaguauugcc     2040

uuagcuuuuc uagcaacagg agguguguua guauuuuuag caacgaacgu gcacgcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10262
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10262
gggagaaagc uuaccauggg aaaacgauca gcaggaucua uaauguggcu cgcaucauua       60

gccguaguua uagcaugugc uggagcagua acaagacgag guagcgcaua cuauauguau      120

cuggauagaa augaugcggg agaagcuaua ucguuuccaa caacacuagg uaugaauaaa      180

uguuauauac aaauaaugga uuuaggacau acaugugaug caacaauguc auaugaaugu      240

ccaaugcuag acgaaggagu ugaaccagac gacguagacu gcugguguaa uacaacaagu      300

acuuggguag ucuauggaac gugccaucau aaaaaaggug aagcaagaag gucccgaagg      360

gcaguuaccu uacccaguca uucaacuaga aaacuucaga caagaucaca aaccuggcua      420

gaaucacgag aguauacaaa acauuuaaua agagucgaaa acuggauauu uagaaauccu      480

ggauucgcac uagccgcagc ugcaauagca uggcuccuag gauccuccac aagucaaaaa      540

guaauauauu uaguaaugau ccuacucauu gcaccagcau auucuauaag guguaucgga      600

guaagcaauc gagacuuugu ugagggaaug aguggcggua cauggguaga uguuguacua      660

gaacacggcg gcugcguaac aguaauggcu caagauaaac cuacaguaga uauagaauua      720

guaacuacua caguaucuaa cauggcagaa guaagaucuu acuguuauga agccucaaua      780

agcgauaugg ccucagauuc aagaugucca acacaaggag aagcauacuu agauaaacaa      840

ucugauacac aauacguaug caagagaaca cuaguagaua gaggaugggg uaaugguugu      900

ggauuauucg gaaaaggaag ccuaguaacu ugugcaaaau uugcauguag uaaaaaaaug      960

acgggaaaaa guauccaacc ugaaaauuua gaauauagaa uaaugcuauc aguucauggc     1020

agucaacaua gcggaaugau uguaaaugau acaggucaug aaaccgacga aaacagagca     1080

aaaguagaaa uuaccccaaa uaguccuaga gcagaagcca cuuuaggagg cuuuggaucc     1140

cuaggucuug auugugaacc aagaacagga uuagauuuuu cggacuuaua uuaccuuaca     1200

augaauaaua aacacuggcu aguucauaaa gaaugguucc augacauacc uuuaccuugg     1260

caugcaggag cagacacugg cacaccacac uggaacaaua aagaagcgcu cguagaauuc     1320

aaagacgccc augcaaaaag acaaacuguu guaguauugg gaucacaaga aggcgcugua     1380

cauacagcau uagcaggagc ucuugaagca gaaauggaug gagcgaaagg aagacuauca     1440

ucggggcacu uaaaaugcag auuaaaaaug gauaaguuac guuuaaaagg gguuagcuac     1500

ucucuuugca cagcagcauu uaccuuuaca aaaauaccag ccgaaacacu acacgguacc     1560

guaacaguag aagugcaaua ugcuggaacu gaugguccau guaaaguacc ugcacagaug     1620

gcuguagaua ugcaaacacu aacgccagua ggaagauuaa uaacagcaaa uccaguaaua     1680

acugaaucaa cagaaaacuc aaaaaugaug uuggaacuag auccaccauu uggagauagu     1740

uauauaguaa uaggaguagg agaaaaaaaa auaacucacc acuggcauag aucagguagu     1800

acguuaggua aagcauuuuc gacaacauua aaaggagcac agagauuagc ugcacuugga     1860

gauacggcuu gggauuucgg aucaauagga ggaguauuua auaguauugg aagagcggua     1920

caucaaguuu uugguggagc auuuagaacc cuauuuggag gaaugucuug gauuacacaa     1980

ggacuuaugg gagcauuauu auuauggaug ggaguaaacg cucgagacag aucaaucgca     2040

uuagcauuuu uagcgacagg aggaguacua guauuucucg caacaaacgu acacgcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10263
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10263
gggagaaagc uuaccauggg aaaaagaagu gcaggaucaa uaaugugguu agcaucacug       60

gcgguaguua uagcaugcgc aggagcuaua acaaggaggg gaucagcaua cuauauguau      120

uuagacagga gugaugcagg aaaagcaauc ucauuugcaa ccacacuagg uguaaauaaa      180

ugucaugucc aaauaaugga ccuaggucau augugugaug cuacaauguc guaugaaugc      240

ccaaugcuug acgaaggugu ggaaccagau gacguagauu gcuggugcaa cacaacaagc      300

acauggguag uuuaugggac augucaccau aagaaaggug aagcacgaag aucacggcga      360

gcaguaacuu uaccaucaca uucaacaagg aaacuacaaa caagaucaca aacaugguua      420

gaaucaagag aguauacaaa acaucuaauu aaagucgaaa auuggauauu uagaaauccu      480

ggauuugccc uaguugcagu ugcaauagcu uggcuucuug gaagcucaac aucccaaaaa      540

guaauuuauu uaguaaugau acuacugauc gcuccugcau acaguauaag auguauagga      600

guaaguaaua gagacuucgu ggaaggaaug uccggaggua cuuggguuga uguaguauua      660

gaacacggug gaugcguaac aguaauggca caagauaaac cuacaguaga cauagagcua      720

guaacaacaa cuguaucgaa uauggcugaa guuagauccu auuguuauga agcaaguaua      780

ucggauaugg caucagacag uagaugucca acacaaggag aagcauacuu agauaaacaa      840

agcgauacac aauauguaug uaaaagaaca uugguugaua gaggaugggg aaauggaugu      900

ggauuauucg gaaaaggaag ucuaguaacu ugugcaaaau ucacguguuc aaagaaaaug      960

acaggaaaau caauacagcc ggaaaaucua gaauauagaa uaaugcuauc aguccaugga     1020

agccagcauu caggaaugau aggauacgaa acagaugaag auagagcaaa aguagaagua     1080

acuccaaauu caccaagagc agaagcaacu uuaggcgguu uugguuccuu agguuuagac     1140

ugcgaaccaa gaacaggauu agauuuuuca gauuuguacu accuaacuau gaacaauaaa     1200

cauuggcuag uucauaaaga augguuucau gacauuccau uacccuggca ugcuggagca     1260

gacacaggaa cuccacauug gaauaauaaa gaagccuugg uagaguuuaa agaugcacac     1320

gcaaaaaggc agacuguagu aguauuaggc ucucaggaag gggcgguaca cacagcauua     1380

gcaggagcau uagaagcaga aauggaugga gcgaagggua gauuauuuag uggccauuua     1440

aaguguagau uaaaaaugga uaaauuaaga uuaaaaggag uaucauauag ucuuuguaca     1500

gcagcuuuca cauuuacuaa agugccagca gaaacauuac auggaacagu aacaguugaa     1560

guacaauaug cuggaacaga uggaccaugu aagauaccag uccagauggc aguagacaug     1620

caaacucuaa caccagucgg gagauuaauu acugcaaacc cgguaauaac agaaagcaca     1680

gaaaacagca aaaugauguu agaauuagac ccaccauuug gugacaguua uauaguaaua     1740

gguguaggag acaagaaaau uacucaucac uggcaucgaa guggaagcac ucuagguaaa     1800

gcauucucua caacacuaaa aggagcacaa aggcuagcag cacuagguga cacagcaugg     1860

gacuuuggau ccauaggagg aguuuuuaac ucaauaggua gagcugugca ucaaguauuc     1920

ggaggagcau uucguacuuu auucggagga augaguugga ucacacaagg auuaauggga     1980

gcacuauuau uauggauggg agucaaugca agagauagau caauagcacu agcauuuuua     2040

gcaacuggag gaguguuagu auuucuagcu acuaauguac acgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10264
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10264
gggagaaagc uuaccauggg aaaaagauca gcaggaagua uaaugugguu agcaagucua       60

gcaguaguaa uagcaugcgc aggagcugua acgcgaaggg gaucagcaua uuauauguau      120

uuagauagaa augacgcagg agaagcaaua ucauuuccca cuacauuagg aaugaacaaa      180

uguuauauac aaauaaugga cuuaggacau augugugaug caacaauguc auacgaaugu      240

ccaauguuag acgaaggcgu agaaccagau gauguagauu gcuggugcaa uacuacaagu      300

acauggguag ucuacggaac augucaucau aagaaaggag aagccagaag aagcagacga      360

gcaguuacac uaccaucaca uaguacgaga aaauuacaaa caagaucaca aaccuggcua      420

gaaaguagag aauauacaaa acauuuaaua agagucgaaa auuggauauu uagaaaucca      480

ggauuugcau uagcagcggc cgcaauagca uggcuacuug guucuucaac aucacaaaaa      540

guaauauauu uaguaaugau uuuacuaauc gcaccagcau acucaauaag auguauaggc      600

guaagcaaca gagauuuugu agaagggaug aguggaggaa cuuggguaga uguuguauua      660

gaacacggcg guugcgucac aguaauggca caagacaaac caacaguaga uauagaauug      720

guaacaacaa ccguaaguaa uauggcagaa guaagaucau auuguuacga agcaucaaua      780

agugauaugg cuucagauuc aagauguccu acucaaggag aggcuuacuu agacaaacaa      840

ucagacacuc aauacgucug uaaaagaaca cucgucgaua gaggaugggg uaauggaugc      900

ggacuauuug gaaaaggauc auuaguaaca ugugcaaaau ucgcaugcag uaagaaaaug      960

acaggaaaaa gcauacaacc ggaaaaucuu gaauacagaa uaaugcuuuc aguucaugga     1020

ucacaacaua guggaaugau agucaaugau acaggucaug aaaccgauga aaauagagca     1080

aagguagaaa uaacaccgaa caguccaaga gcagaagcaa cacucggagg auuuggauca     1140

uuagguuuag auugugaacc aagaacggga cuagauuuua gcgauuuaua uuaccuaacu     1200

augaauaaua aacauugguu aguacauaaa gaaugguuuc augauauacc uuuacccugg     1260

caugcaggag cagauacugg aacuccucau uggaauaaua aagaagcauu aguagaauuu     1320

aaagacgcac acgcuaaaag gcaaacaguu gugguuuuag gaagucagga aggagcagua     1380

cacacagcau uagcgggagc auuagaagcc gaaauggacg gagcaaaagg ccgauuauca     1440

ucaggacauc uuaaauguag acuaaaaaug gauaaacuaa gauuaaaagg aguuucuuau     1500

ucauugugua cagcagcauu uaccuuuaca aaaauaccgg cugaaacuuu gcacggaacg     1560

gugacaguag aaguacaaua ugcagguaca gacgguccuu guaaaguacc agcccagaug     1620

gcaguagaca ugcaaacacu aacgccagua gguagauuaa ucacagcaaa cccaguaaua     1680

acggaaagca cagaaaauuc uaaaaugaug uuagaauuag auccaccuuu cggagacuca     1740

uauauaguca uaggaguggg ugagaaaaag auaacacacc auuggcacag aagcggaagu     1800

acauuaggaa aagcauucuc aacaacacua aaaggagcac aacgucuagc ugcauugggu     1860

gauacugcau gggauuuugg cagcauaggu ggaguauuua auagcauugg uagagcugug     1920

caucaaguuu ucggaggagc auuccgaaca uuauuuggcg gaaugucaug gauaacacaa     1980

ggauuaaugg gagcacuauu auuauggaug ggaguuaaug caagagauag gaguaucgca     2040

cuagcauucu uagcaacagg aggaguauua guguuuuuag caacuaaugu acacgcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10265
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10265
gggagaaagc uuaccaugac aucaguugga auaguaggcc uacuuuuaac aacagcaaug       60

gcagcaguua cauuaccaag ucauaguaca agaaaacuac agacaagaag ccaaacaugg      120

uuagaaucca gagaguacac uaaacauuua aucaggguag aaaauuggau auuuagaaau      180

ccaggauuug cacuagcagc agccgcaaua gcauggcuau uaggaucuuc uacaucacaa      240

aaaguaauuu auuuaguaau gauacuauua auagcaccug cuuauaguau aagguguauu      300

ggaguuucaa aucgagacuu uguugaagga auguccggug gaacaugggu agacauagua      360

uuagaacaug gcgguugugu aacuguaaug gcgcaagaua aaccaacugu agauauagag      420

cuaguaacca ccacaguaag uaauauggca gaaguuagaa guuauuguua ugaagcguca      480

auaagugaca uggcaaguga uagccgaugu ccaacucaag gcgaagcaua uuuagacaaa      540

caaucagaua cucaauaugu uugcaaaaga acauuaguag auagagguug gggaaauggc      600

uguggauuau uugguaaagg aucauuagua acuugcgcaa aauuugcaug uucaaaaaaa      660

augacaggaa aaucuauaca accagaaaau cuagaauaca gaauaaugcu aaguguacac      720

ggaucccaac auucaggaau gauuguaaau gauacuggac augaaacaga ugaaaauaga      780

gcaaaaguag aaauuacacc aaacucaccu cgagcagaag caacauuagg cgguuuugga      840

ucuuuaggau uagauugcga accacguaca gguuuagauu ucucagaucu cuauuaucuc      900

accaugaaua auaaacauug gcuaguacau aaagaauggu uucaugauau uccauuacca      960

uggcacgcgg gggcagauac aggcacucca cauuggaaca auaaagaagc auuaguagaa     1020

uuuaaagacg cccaugcuaa aagacaaaca guuguaguac uaggaagcca agaaggagcc     1080

guacauacag cauuagcugg ggcacuagaa gcagaaaugg auggagcaaa aggaagauua     1140

aguucugguc aucuaaaaug uagauuaaaa auggacaaac uacgauuaaa agguguuagc     1200

uauagcuuau guacagcagc auuuacauuu acaaaaauac cugcagaaac uuuacaugga     1260

acaguaacag ucgaaguaca auacgcagga acagauggcc cauguaaagu uccagcacaa     1320

auggccguag acaugcagac auuaacaccc guaggaagau uaauuacugc aaauccagua     1380

auaacggaau ccacagaaaa cucaaaaaug auguuagagc uugacccacc guuuggagau     1440

aguuauauag ugauaggagu aggagaaaaa aaaauaacuc accauuggca cagaaguggu     1500

ucuacaauag gaaaagcauu cgaagcaacg gucagaggug caaaaagaau ggcuguauua     1560

ggagacacag caugggacuu uggcagcgua ggaggagcac uaaacucauu aggaaaagga     1620

auacaccaaa uuuucggagc ggcauucaaa agucuuuuug gaggaauguc uugguuuuca     1680

caaauauuaa uaggaacacu acuaaugugg uuaggacuaa auacaaaaaa uggaucaaua     1740

ucccugaugu gccuagccuu aggaggcgua cuaauauuuu uauccacugc aguaucagcu     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10266
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10266
gggagaaagc uuaccaugac aagcguagga auaguaggau uauuauuaac aacagcaaug       60

gcugcuguca cccuaccaag ucauaguaca agaaaacuac aaacuagauc acaaacaugg      120

cuagaaaguc gagaauauac caaacauuua auaagagugg aaaauuggau cuuuagaaau      180

ccaggauuug cucuggcugc agcggcaaua gcaugguuac uuggaucaag uacaucacaa      240

aaaguaauau auuuaguuau gauauuacuu auagcaccag ccuauaguau acguuguaua      300

ggcguuagca auagagauuu ugucgaagga augagcggag gaacaugggu agacguagua      360

cuagaacaug gaggaugugu aaccguaaug gcacaagaca aaccuacggu ggauauagaa      420

uuaguaacga caacaguauc uaauauggca gagguacgaa gcuacuguua ugaagcaagu      480

auaucagaua uggcaaguga uagcagaugu ccaacacaag gagaggcaua ccuagauaaa      540

caaagugaua cacaauaugu gugcaaaaga acccuaguag aucgaggcug gggaaauggc      600

ugugguuuau uugguaaggg aucauuagua acaugugcaa aauuugcaug cucuaagaaa      660

augacaggaa aaucaauaca accugaaaau cuugaauaua gaauaauguu gaguguacau      720

ggaucucaac auagcggaau gauugugaau gacacaggac acgagacaga ugaaaauaga      780

gcuaaaguag aaauaacacc aaauucacca agagcagaag caacauuagg aggauucgga      840

ucguuagguu uagauuguga acccagaacc ggauuagauu uuagcgauuu auauuaccua      900

acaaugaaca auaaacauug guuaguacau aaagaauggu uucaugauau ucccuuaccu      960

uggcaugcug gagcagauac aggaacacca cacuggaaca auaaagaagc auuaguagaa     1020

uuuaaagaug cacacgcaaa aagacaaacu gugguaguac uaggaaguca agaaggugca     1080

guacacacag cauuagcagg agcauuagaa gcagaaaugg acggagcuaa aggaagauua     1140

ucuucaggac accuaaagug uagauuaaaa auggacaaac ugagacuaaa agguguaagu     1200

uauaguuuau guaccgcagc uuuuacauuu acaaaaauac cagcagaaac acuacacgga     1260

acaguaacgg ucgaagugca auaugcagga acagauggac caugcaaagu gccagcacaa     1320

auggcgguag acaugcaaac auuaacacca guaggaagau uaauuacugc aaauccaguu     1380

auaaccgaaa gcacagaaaa cucaaaaaug auguuagaac uagauccacc uuuuggagau     1440

aguuauauag uaauaggagu aggugaaaag aaaaucacac accauuggca uagaaguggc     1500

ucaacaauag gaaaagcauu cgaagcaaca guaagagggg caaaaagaau ggcuguacua     1560

ggagauacag caugggauuu uggaucaguc ggaggagcau uaaacagcuu gggaaaagga     1620

auacaucaaa uauuuggagc ugcauuuaag aguuuauucg gcggaaugag uugguuuagu     1680

caaauauuaa uaggaacuuu acucaugugg uuaggguuaa augccaaaaa uggcucgauu     1740

agucuaaugu guuuagcuuu aggaggagua uuaauauuuc uaagcacagc ggucucagcu     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10267
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10267
gggagaaagc uuaccaugac aaguauagga auaauaggac uacuauuaac aacagccaug       60

gcagcaguaa cacuaccuuc acacucgacc aggaaacuac aaacccgaag ucaaaccugg      120

uuagaaagua gagaauacac uaaacaucua auaaaaguug aaaauuggau auuuagaaau      180

ccaggauuug cuuugguugc aguugcuaua gcauggcuau uaggaagcag cacaucucaa      240

aaaguuauuu aucuaguuau gauacucuua auagcuccug cauauaguau acgcuguaua      300

ggaguaucaa auagagauuu uguagaagga augucaggag gcacaugggu agauguugua      360

cuagaacacg guggaugugu aacaguaaug gcacaagaua aaccaacggu agacauagaa      420

uuaguaacaa caaccguaag uaauauggca gaaguacgau cauacugcua ugaagcauca      480

auaagcgaca uggcaaguga uucuagaugc ccaacacaag gugaagcaua ucuagauaaa      540

caaucugaca cgcaguaugu auguaaacgc acgcuaguag auagaggaug ggguaacggu      600

uguggucuau uugguaaagg aucucuugua acaugcgcaa aauucacaug uucuaagaaa      660

augacaggaa aaagcauuca gccagaaaau cuagaauaca gaauaaugcu aucuguucau      720

ggaucucaac auuccgguau gauaggauau gaaacugaug aagauagagc uaaaguagaa      780

guaacaccaa auaguccaag agcugaagca acacuaggag gcuucgguag uuuaggguua      840

gauugugaac cgagaacagg acuugacuuu agugaccuau auuaucuuac aaugaacaau      900

aaacauuggu uaguccauaa agaaugguuc cacgauauac cacuaccaug gcaugcuggu      960

gcagauacag gaacaccaca cuggaauaau aaagaagcuc uaguagaguu caaagaugca     1020

caugcaaaaa gacaaacagu aguaguacua ggaagucaag aaggggcagu acauacagca     1080

uuagcaggag cauuagaagc agaaauggac ggagcaaaag gacgucucuu cuccggacac     1140

cuaaaaugca ggcuaaaaau ggacaaauua agguuaaaag gaguaucuua uucacuuugu     1200

acagccgcau ucacauuuac aaaaguacca gcagaaacac uacauggaac uguaacaguu     1260

gaaguucaau augcuggaac agauggacca uguaaaauac cuguacaaau ggcaguagau     1320

augcaaacuu uaacaccagu agguagauua auaacagcaa acccuguuau aacagaaagu     1380

acagaaaaca gcaaaaugau gcuagaacua gacccuccau ucggagacuc uuacauagua     1440

auuggaguag gagacaagaa aauaacacau cauuggcacc gaucuggcuc aacaauagga     1500

aaagcauuug aagcaacagu aagaggagca aaaagaaugg caguauuagg agauacugcc     1560

ugggauuuug gaucaguagg aggaguauuc aauagcuuag gcaaaggcau acaucaaaua     1620

uuuggugcug cauucaaaag uuuauuuggg ggaaugucau gguuuucaca aauucuaaua     1680

ggaacacuau uaguauggcu aggauuaaac acgaaaaaug gaagcauuag ucuaacaugu     1740

cuagcucuag guggaguaau gauauuccua ucaacagcag uaucagcaug aggacuaguu     1800

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     1860

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  10268
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10268
gggagaaagc uuaccaugac aucaguagga auagucggau uauuauuaac aacagcaaug       60

gccgcaguaa cauuaccaag ccauaguacu agaaaauuac aaacacgaag ccaaacaugg      120

uuggaaucaa gagaauacac aaaacaucuu auaagagugg aaaauuggau auucagaaau      180

ccaggauuug cacuagcagc agcagcuaua gcauggcuuc uaggcaguag uacaucacaa      240

aaagucauuu auuuaguaau gauauuacuu auagcuccag cauauucuau ucguuguaua      300

ggaguaucaa acagagacuu uguugaagga augaguggag gaacuugggu agauguaguu      360

uuagaacaug guggaugugu aacaguaaug gcacaagaua aaccuacagu agauauagag      420

cuaguuacaa caacgguauc aaauauggcc gaaguaagga guuauuguua ugaagcauca      480

auaucagaua uggcaucaga uaguagaugu ccaacacagg gcgaagccua ucuagauaaa      540

caaucagaca cacaauaugu auguaaaagg acacuggugg auagaggaug gggcaacgga      600

uguggcuuau uuggaaaagg uaguuuaguc acaugugcaa aauuugcaug uucuaagaaa      660

augacaggua aaucuaucca accagaaaau cuagaauaua gaauaaugcu uagcguucau      720

ggaagucaac acagugguau gauugugaau gauacuggac augaaacaga cgaaaauaga      780

gcaaaaguag aaauaacacc aaacucaccc agagcugaag caacacuagg aggauucgga      840

ucacuuggac uagauuguga accuagaacu ggacuugauu ucagugauuu auacuacuua      900

acuaugaaua auaaacauug guuaguacau aaagaauggu uccacgacau uccacuaccu      960

uggcaugcug gugcagauac aggaacgcca cauuggaaua auaaagaagc acucguagag     1020

uucaaagaug cacaugcaaa acgacaaacg guaguuguau uaggaaguca agaaggcgca     1080

guccauacag cauuagcugg agcacuagaa gcugaaaugg auggagcaaa agguagauua     1140

aguuccggac accuaaaaug uagacuaaaa auggauaaau uaagacugaa agguguaagc     1200

uauagucugu gcaccgcugc auuuacauuc acaaaaauac cugcugaaac auuacaugga     1260

acaguaacag uugaaguaca auaugcagga acagacggac cuugcaaagu acccgcacag     1320

auggcuguag acaugcaaac auuaacccca gugggaagac uaauaacagc aaauccagua     1380

auaacagaaa gcacugaaaa uucaaaaaug auguuagaac ucgauccacc auuuggggau     1440

agcuauauag uaauaggagu aggcgaaaag aaaauaacac accacuggca uagaaguggu     1500

aguaccauug gaaaagcauu ugaagcuaca gucagaggag caaaaagaau ggcaguacuu     1560

ggcgauacag cuugggauuu ugguucugua ggcggagcuu uaaacaguuu aggaaaagga     1620

auacaucaaa ucuuuggagc agccuuuaaa aguuuauuug gaggaaugag uugguuuucc     1680

caaauuuuaa uaggaaccuu auuaaugugg cuagguuuaa auacgaaaaa ugggagcauc     1740

agcuuaaugu gucuagcacu ugguggggua uuaauauuuc uaagcacagc gguaucugca     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10269
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10269
gggagaaagc uuaccauggc cauaagcgga guaccaguau uaggauucuu cauaaucgca       60

guacuaauga gugcacaaga aaguugggca gcagugacau uaccaaguca caguacaaga      120

aaauuacaaa cacguaguca aacaugguua gaaagucgag aauauacaaa acaucuaaua      180

cguguagaaa acuggauauu uagaaaucca ggauuugcuu uggcagcagc ugccauagca      240

uggcuucuag gaagcucaac aucacaaaaa guuauauauc uagucaugau ucuucuaauc      300

gccccugcau auucaauaag auguauagga gucucaaaca gagauuucgu agaaggaaug      360

ucaggaggaa cauggguaga cauaguccuu gaacacggag gauguguaac uguuauggcc      420

caagauaaac cuacuguaga uauagaauug gucacaacua caguaagcaa cauggcagaa      480

guaagaucau auuguuauga agcuagcaua ucagauaugg caucagauuc aagaugccca      540

acacaaggug aagcauaucu agauaaacaa ucagauacac aauacguaug uaaacgaaca      600

uuaguagaua gaggaugggg aaauggaugu ggacuuuuug gaaaaggauc auuaguaaca      660

ugcgccaaau uugcguguuc aaagaaaaug acuggaaaau caauacaacc ggaaaaccua      720

gaauauagaa uaauguuaag uguucaugga ucacaacauu cagguaugau aguuaaugau      780

acaggacaug aaacagauga aaacagagca aaaguagaaa uaacaccaaa uucaccaaga      840

gcagaagcaa cauuaggcgg uuucggaagu uuaggacuug auugugaacc uagaacaggu      900

uuagauuuuu cagaccuaua uuauuugaca augaauaaca aacacugguu aguacacaaa      960

gaaugguucc augauauacc acuaccaugg caugcuggug cggauacagg aacuccccau     1020

uggaauaaua aagaagcguu aguagaauuu aaagacgcac acgcaaaaag acaaacugua     1080

guaguauugg gaucucaaga aggagcagua cacacugcau uagcaggagc acuagaagca     1140

gaaauggaug gagcuaaagg cagacuuagc aguggacauu uaaaaugucg uuuaaaaaug     1200

gacaaauuaa gauuaaaagg gguauccuac ucccuaugca cagcugcuuu uacuuucaca     1260

aaaauuccug cagaaacacu ucauggaacc guaacaguag aaguacaaua cgcgggaaca     1320

gaugggcccu gcaaaguacc cgcacaaaug gcaguagaua ugcaaacauu aacaccugua     1380

ggcagauuga uaacugcuaa uccaguaaua acagaaucaa cagaaaauag uaaaaugaug     1440

cuagaauuag acccaccauu uggcgauagu uauauaguca uuggaguugg ggagaagaaa     1500

auaacucauc acuggcacag aucaggaagu acuauaggaa aagcauucga agcaacagug     1560

agaggagcaa aaagaauggc aguauuaggu gauacagcuu gggauuuugg aucaguagga     1620

ggagcacuaa acucucuagg uaaaggaauc caucaaauuu uuggugcagc cuuuaaauca     1680

uuauuuggag gaaugucaug guucagucaa auauuaauag guacauuauu aauguggcug     1740

ggacuaaaua cuaaaaacgg uaguauaagc cuaauguguc uagcacucgg ugguguacua     1800

auauucuuaa guacagcagu cucagcauga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10270
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10270
gggagaaagc uuaccauggc gauaagugga guaccagugu uaggauuuuu uauaauagca       60

guacuaaugu cugcucaaga aucaugggca gcagucacau uaccaucaca cucaacuagg      120

aaauuacaga cccgaucgca aaccuggcug gaaucaagag aauauacaaa acacuuaaua      180

agaguagaaa auuggauauu uagaaauccc ggauucgcac uagcagcggc ugcaauugcu      240

ugguuacuug gauccaguac aucccaaaaa guaauauauu uaguaaugau acuucuuauc      300

gcaccagccu auaguauuag auguauugga guaucaaaua gagauuuugu agaaggaaug      360

ucaggaggua cuuggguuga ugugguacua gaacacggcg gaugugucac aguaauggca      420

caagauaaac ccacaguaga uauagaauua guaacgacaa cagugucaaa uauggcagaa      480

guaagaucau auugcuacga agcaaguaua agugacaugg caucagauuc acgaugucca      540

acacaaggag aagcauauuu agauaaacaa ucagacacac aauauguaug uaaaagaaca      600

uuagucgaua gggguugggg aaacggaugc ggcuuauuug gaaaaggauc cuuaguaacc      660

ugugcaaaau ucgcauguuc gaaaaagaug acaggaaaau cuauacaacc agaaaauuua      720

gaauauagaa uaaugcuauc aguacacgga ucucaacauu cagguaugau aguaaaugau      780

acuggacaug aaacagacga gaacagagca aaaguagaaa uaacuccaaa uagcccacgu      840

gcggaggcca cacuaggagg auuuggaagc cuggggcuag acugugaacc uagaacagga      900

cuagauuucu cagaccuaua uuauuuaaca augaauaaca aacauugguu aguccacaaa      960

gaaugguuuc augauauccc acuaccaugg cacgcaggug cagauacggg uacaccacau     1020

uggaauaaca aagaagcauu agucgaauuu aaagaugccc augcaaaaag acaaacagua     1080

guaguacuag guucacaaga aggagcugug cauacagcac uagcuggugc auuagaagca     1140

gaaauggaug gagcaaaagg aagauuauca agcggacauu ugaaaugucg acuaaaaaug     1200

gauaaacuua ggcuuaaagg uguaagcuac ucuuuaugua ccgcagcauu cacguucaca     1260

aaaauaccag ccgaaacauu acacgguacu guaacaguag aaguacaaua cgcagguaca     1320

gauggaccau guaaaguacc agcucaaaug gcaguagaca ugcaaacuuu aacaccagua     1380

gggagauuaa uaacagcgaa uccaguaauu acagaaucca cagaaaacag uaaaaugaug     1440

cuagaauuag auccacccuu uggagacuca uauaucguaa uagguguagg ugaaaaaaaa     1500

auaacacauc acuggcaucg aucuggauca accauaggaa aagcauuuga agcuacagua     1560

agaggagcga aaaggauggc aguuuuagga gacacugcau gggauuuugg uaguguagga     1620

ggagcauuaa auucauuggg uaaaggcaua caucaaauau ucggagcagc auuuaaaagc     1680

uuauuuggag gaaugaguug guuuucacaa auacuaauag gaacuuuacu uaugugguua     1740

ggucuaaaug cuaaaaaugg cucaauauca cuaauguguu uagcacuagg uggaguacua     1800

auauuuuuaa guacagcugu aucagcauga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10271
<211>  2020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10271
gggagaaagc uuaccauggc aauaucaggu guaccagucu uagguuucuu uauaauagca       60

guauuaaugu cagcacaaga aagcugggcc gcaguaacuc uacccaguca cucgacaagg      120

aaacuacaaa ccagaucaca aacaugguua gaaaguagag aauauacaaa acaccuuauu      180

aaaguagaaa auuggauauu uagaaauccu ggguucgcgu uagucgcagu agcaauagca      240

uggcuauuag gaagcagcac aagucaaaaa guaauauacc uaguaaugau auuacuaauc      300

gcgccagcau auaguauacg uuguauagga guuucaaaca gagacuuugu agaaggaaug      360

ucaggaggua cauggguaga ugucguauua gaacauggag gaugugucac aguuauggcu      420

caagauaaac caacaguaga uaucgaauua guaacaacua cugucaguaa uauggccgag      480

guuagaucau acuguuacga agcaucaaua ucagauaugg caagugauag uagaugccca      540

acacaaggag aagcauauuu agacaaacaa ucagauacuc aauacguaug uaaacgaaca      600

cuaguagaca gagguugggg aaacggaugu ggauuauuug gaaaaggaag uuuaguaaca      660

ugugcaaaau uuacuugcuc aaaaaaaaug acuggaaaau caauucaacc ggaaaaucua      720

gaauaucgaa uaaugcuaag uguacaugga agccaacauu caggaaugau uggcuaugaa      780

acugaugaag acagagcuaa aguugaagug acaccaaacu caccuagagc agaagcaaca      840

uuaggagguu ucgguucauu aggauuagau ugcgaaccac gaacagguuu agauuucagu      900

gaucuauauu auuuaacaau gaacaauaaa cauuggcuag uacauaaaga augguuccau      960

gauauaccac ucccauggca ugcgggugca gacacaggca cuccacacug gaacaauaaa     1020

gaagcuuuag uagaauuuaa agaugcacac gcaaaaagac agacaguagu uguacuagga     1080

ucacaagaag gagcaguaca caccgcacuu gcaggcgcau uagaagcgga aauggacgga     1140

gccaaaggua gauuauuuag uggacauuua aaauguagau uaaaaaugga uaaauuaaga     1200

cuaaaaggag uaagcuacuc auuaugcaca gcagccuuua cauuuacaaa aguacccgcu     1260

gaaacauugc acggaacagu uacaguugaa guucaauacg caggaacaga uggaccaugu     1320

aaaauaccag uacaaauggc agucgacaug caaacauuaa caccaguagg ucgacuaaua     1380

acagcaaacc ccguuauaac cgaaucuacu gaaaacucga aaaugaugcu agaacuagac     1440

ccaccguuug gagauucaua uauaguaaua ggcguaggag auaaaaaaau aacacaucau     1500

uggcacagau ccggaagcac aauaggaaaa gcauuugaag cuacaguaag aggagcuaaa     1560

agaauggcag uauuaggaga uacagcaugg gacuuuggaa guguaggagg uguauuuaac     1620

aguuuaggaa aaggaauaca ucaaauauuu ggcgcagccu uuaaaagccu auucggagga     1680

augagcuggu uuucacaaau ucuaaucgga acucuacuag uguggcuagg acuuaauaca     1740

aaaaauggua gcauaaguuu aacaugucua gcauuaggag guguuaugau auuucuauca     1800

acagcgguaa gugcaugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     1860

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1980

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2020


<210>  10272
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10272
gggagaaagc uuaccauggc aauuucugga guuccaguau uagguuuuuu uauuauagcg       60

guacuaauga gugcacaaga aagcugggca gcaguaacau uaccuaguca uaguacuaga      120

aaacuacaaa caagaucaca aacaugguua gaaucgcgag aauauaccaa acauuuaaua      180

agaguagaga acuggauuuu cagaaaucca ggcuucgccc uagcagcagc agcaauagca      240

ugguuauuag gaaguucaac aucacaaaaa guuauauauc uagucaugau auuauugaua      300

gcgcccgcau auucaauacg auguaucgga guaucaaaua gagauuuugu agaaggaaug      360

ucuggaggaa cuuggguaga cgucguacua gaacacgggg gauguguaac aguaauggcu      420

caagauaaac cuacaguaga uauagaauua guaacgacaa caguaagcaa cauggcagaa      480

gucagauccu auuguuauga agccaguauu agcgacaugg cgagcgauag cagaugucca      540

acacaagggg aagcuuauuu agauaaacaa ucagacacac aauacguaug uaaaagaaca      600

uuaguagaua gagguugggg aaaugguugu ggacuuuuug gaaaaggaag uuuaguaaca      660

ugugcaaaau uugcaugcuc uaaaaaaaug acaggcaaau caauacaacc agagaaccuc      720

gaauauagaa uaauguuaag cguacaugga agucaacaua gcgguaugau aguaaaugac      780

accggacacg aaacagacga aaauagagca aaaguagaaa uaacgccaaa uaguccgcgc      840

gcagaagcaa cauuaggagg auucggauca cuaggauuag acugugaacc aagaacagga      900

cuugauuuuu cugaucuaua cuaucuuaca augaauaaua aacauugguu aguucauaaa      960

gaaugguucc augauauucc uuuaccaugg cacgcaggug cagauacagg aacaccacau     1020

uggaauaaua aagaagcauu aguagaauuc aaagaugcuc augcuaaacg ucaaacugua     1080

guuguauuag gaagccaaga aggagcaguu cauacugcau uagcaggagc auuagaagcu     1140

gaaauggaug gugcaaaagg uagauugagu aguggacauu uaaaauguag acuaaaaaug     1200

gacaaauuac gacuaaaagg aguuucauau agucuaugua cugcugcauu uacauucaca     1260

aaaauaccag cugaaacauu acacggaacc guuacaguug aaguacaaua cgcaggcaca     1320

gauggaccau gcaaaguucc agcacaaaug gcaguagaca ugcaaacauu aacaccagua     1380

ggaagacuaa uuacagcaaa uccuguaaua acagaaucaa cugaaaauag caaaaugaug     1440

uuagaauuag auccaccauu uggagauuca uauauuguga uaggaguagg agaaaaaaaa     1500

auuacacacc acuggcauag aucuggguca acaauaggua aagcauucga agcuacugua     1560

agaggagcaa aaagaauggc aguacuugga gauaccgccu gggauuucgg uaguguagga     1620

ggagcacuaa acucacuugg aaaaggcaua caucaaauau uuggagcagc auuuaaauca     1680

cuauuuggug gaaugucaug guuuucacag aucuuaauag guacacuacu aauguggcua     1740

ggacuuaaua caaaaaaugg aucaauauca uuaauguguu uagcauuagg aggaguuuua     1800

auuuuuuuaa gcacagcagu aagugcauga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10273
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10273
gggagaaagc uuaccaugga uuggacaugg auauuauucu uaguugcagc ugcaacaaga       60

guacauagug caguaacauu gccgucacac ucaacaagga aauuacaaac aagaagccaa      120

acaugguuag aaagccgaga auacacaaaa cauuuaauua gaguagaaaa cuggauauuu      180

agaaauccag gauuugcucu agcggcagca gcaauagcau gguuacuugg aucaucaaca      240

agccagaaag uaauauacuu aguaaugaua cuucuuauag cuccagcaua cucaauacga      300

uguauuggag uaagcaauag agauuuuguc gaaggaaugu caggaggaac augggucgac      360

aucguauuag aacauggugg auguguuaca guaauggcac aagauaaacc uacuguagau      420

auagaacucg uaacuacaac uguaucaaac auggcagaag uaagaucaua cugcuaugaa      480

gcaucaauaa gugauauggc aucggauagu agauguccua cacaaggaga agcauauuua      540

gauaaacaau cagauacaca auacguaugu aaacguacuu uaguagauag agguugggga      600

aauggaugcg gauuauuugg aaaagguagu uuaguaacau gugcaaaauu ugcuuguuca      660

aagaaaauga cagguaaaag uauacaacca gaaaauuugg aauauagaau aauguuaucu      720

guacauggaa gccaacauag uggaaugauu guaaaugaua caggacauga gaccgaugaa      780

aauagagcaa aaguagaaau aacuccaaau ucaccacgag cagaagcgac auuaggagga      840

uucgguucau uagguuuaga uugugaacca agaacagggc uagauuuuuc ugaucuauau      900

uaucuaacua ugaacaauaa acauuggcua guacauaaag agugguuuca ugacauacca      960

uuaccauggc augcuggagc agauacagga acaccacauu ggaacaauaa agaggcuuug     1020

guugaauuua aagacgccca cgcaaaaaga cagacaguag ucguuuuagg cagucaagaa     1080

ggagcaguuc acacagcauu agcuggagca uuagaagccg aaauggacgg agcaaaaggu     1140

cgauuaucaa guggacaccu aaaauguaga uuaaaaaugg acaaacuacg acuuaaaggg     1200

guuucauauu cacuauguac agcagccuuu accuuuacaa aaauaccagc agaaacuuua     1260

caugguacag uaacagucga aguccaauau gcagguacag auggcccaug uaaagucccg     1320

gcacaaaugg caguagauau gcagacacua accccaguag gaagacuuau aacagcaaau     1380

ccaguuauaa cagaaucuac ugaaaauuca aaaaugaugu uagaguuaga uccuccauuu     1440

ggagauagcu auaucguaau aggagucgga gaaaagaaaa uaacacauca uuggcauaga     1500

aguggaagua ccauaggaaa agcauuugaa gcaacaguaa gaggagccaa aagaauggca     1560

guacuuggag auacggcaug ggauuuuggc agcguaggag gugcauugaa uaguuuagga     1620

aaaggaauac aucaaaucuu uggugccgca uucaaauccu uauuuggagg aaugagcugg     1680

uuuucacaaa uacuaaucgg aacauuacua augugguuag gauuaaauac uaaaaaugga     1740

agcauaaguu uaaugugccu ggcacuagga ggaguacuaa uauuuuuaag uacagcagug     1800

agcgcuugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10274
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10274
gggagaaagc uuaccaugga uuggacaugg auauuauucu uaguagcugc agcaacccga       60

guacacucag cagucacauu acccagucau agcacaagga aauuacaaac cagaucacaa      120

acuugguuag aaucaagaga auacacaaaa caucucauaa gaguugaaaa cuggauauuu      180

agaaaccccg gcuuugcucu agcagcagca gcaauugcau ggcuacuagg aagcucuaca      240

ucacaaaagg uaauauauuu aguaaugaua cuauuaauag caccagcuua caguauuaga      300

uguauaggag uuucgaauag agacuuugua gaaggaaugu caggagguac auggguagau      360

guaguacuag aacauggcgg augugugaca guuauggcac aagauaaacc aaccguagau      420

auagaauuag ugacgacaac aguaucaaau auggccgaag uaagaucuua uuguuaugaa      480

gcauccauuu cugacauggc cucagauagu agaugcccca cucaaggaga agcauacuua      540

gauaaacagu cagacacuca auacguuugu aaaagaacuc uaguagauag agguuggggu      600

aauggaugug gauuauuugg aaaaggaagc cuaguaacau gcgcaaaauu ugcauguagu      660

aaaaagauga cuggaaaauc aauacaacca gaaaaccuug aauaucgaau uauguuguca      720

guccacggaa gucaacauag uggaaugaua guaaaugaca caggacacga aacagacgaa      780

aauagagcua aaguagaaau aacaccaaau ucuccgagag cugaagcaac acuuggagga      840

uuugguagcu uagguuuaga cugugaacca aggacuggau uggacuuuuc agaucuauau      900

uauuuaacaa ugaauaauaa acacuggcua guacacaaag aaugguuuca cgauauucca      960

uuaccauggc augcaggagc ugacacaggu acaccacauu ggaauaauaa agaagcauua     1020

guagaguuca aagaugcaca cgcgaaaaga caaacaguag uagugcuagg uucacaagaa     1080

ggagcaguac auaccgcauu agcaggagca uuagaagcag aaauggaugg agcaaaaggg     1140

agacuaucaa guggacauuu aaaauguaga uugaagaugg auaaauuaag auugaaaggg     1200

guaucuuaca guuuauguac ugcagcuuuc acauuuacaa aaauaccagc agaaacacua     1260

cauggaacag uaacaguuga aguacaauac gcaggaacag auggacccug caaaguucca     1320

gcucaaaugg caguagauau gcaaacucua acgccaguag gacgguuaau aacagccaau     1380

ccagugauua cagaaucgac agaaaacuca aaaaugaugc ucgaauuaga uccacccuuu     1440

ggagauucau auauuguuau aggaguagga gaaaagaaaa uaacacauca uuggcauagg     1500

agcggcucaa cuauaggaaa agcauucgaa gcaacaguaa gaggagcuaa acgaauggca     1560

guauuaggag auacagcuug ggacuucgga agcguuggag gagcccuaaa uaguuuagga     1620

aaaggaauac aucaaauauu cggagcagca uuuaaaucau uauuuggagg aaugaguugg     1680

uucucacaaa uucuuauugg aacccuauua auguggcuag gcuugaaugc uaaaaaugga     1740

aguauaaguc ucaugugcuu agcacuagga gggguauuaa uauuuuuauc caccgcaguu     1800

ucagcaugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10275
<211>  1999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10275
gggagaaagc uuaccaugga uuggacaugg auauuauuuu uagucgcagc agcaacuaga       60

guacauucug caguuacuuu accaagucau ucaacaagga aacuacaaac aagaucacaa      120

acauggcuag aaucaagaga auacacuaaa caucuaauaa aaguagaaaa uuggauauuu      180

agaaaucccg gcuuugcacu cguggcagua gcaauagcau gguuguuagg gagcucuacc      240

agucaaaaag uuauuuaucu uguaaugaua cuacuaauag caccagcaua uagcauaaga      300

uguauaggag uuagcaacag agacuuugug gaaggaaugu caggaggaac auggguagau      360

gucguacuag aacauggcgg auguguaaca guaauggcac aagauaaacc aacaguagau      420

auagaacuag uaacaacaac uguaucuaau auggcagaag uaagaucaua cuguuaugaa      480

gcaagcauuu cugauauggc aucagauagc agaugcccaa cacaaggaga agcauaucua      540

gauaaacaaa gugauacaca auacguaugc aaaagaacau uaguugacag aggauggggg      600

aaugguugcg gucuguuugg aaaaggaagu uuaguuacau gugcuaaauu uacaugcagu      660

aaaaaaauga cuggaaaaag cauucaacca gaaaauuuag aauaccgaau aauguuaagc      720

guacauggaa gccaacacuc aggaaugaua ggauaugaaa cagaugaaga uagagcuaaa      780

guagaaguca caccgaauuc accaagagcu gaagcaacuc uaggagguuu ugguucacuu      840

gguuuagauu gugaaccaag aacaggacua gacuucucag auuuguauua ccuaacaaug      900

aauaauaaac auuggcuagu ucauaaagaa ugguuucacg acauaccauu gccuuggcac      960

gcaggagccg auacuggaac accacauugg aauaauaaag aagcacucgu agaauuuaaa     1020

gaugcacaug ccaaaagaca aacuguaguc guacuaggaa gucaagaagg ggccguacau     1080

acugcauuag caggcgcacu agaagcugaa auggauggcg caaaagguag acuauucagc     1140

ggacaccuaa aaugucgauu aaaaauggau aaauuaagau uaaagggagu aagcuauucu     1200

uuauguacag ccgcuuuuac auucacuaaa guaccagcag aaacauuaca cgguacagua     1260

accguagaag uccaauacgc aggaacagau ggaccaugca aaauuccagu acaaauggca     1320

guagauaugc aaacguuaac accaguaggu cgauuaauua cagcaaaucc aguaaucaca     1380

gaaucaacag aaaauucgaa aaugaugcuc gaacuagacc cuccauucgg agauaguuau     1440

auaguaaucg gaguaggaga uaaaaaaaua acacaccacu ggcauagaag uggcucaaca     1500

auagggaaag cauuugaagc aacaguaaga ggagcaaaaa gaauggcagu uuuaggugac     1560

acugcauggg acuuugguag uguaggaggu guauuuaaua gcuuaggaaa aggaauccau     1620

caaauauuug gagcagcauu caaaucauua uucgguggua ugucaugguu cucacaaauu     1680

cuaauaggua cacuucuugu uugguuaggu cuaaauacaa aaaauggauc aauuucucua     1740

acauguuuag cuuuaggagg ggucaugauc uucuuaucua cggcaguauc agcuugagga     1800

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     1860

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     1980

uuucagagcc accagaauu                                                  1999


<210>  10276
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10276
gggagaaagc uuaccaugga cuggacaugg auucuauuuc ucguagcagc agcaacaaga       60

guucauucag caguaacauu accaucacau ucaacaagaa aacuacaaac aagaagucaa      120

acauggcuag aaucgagaga auauacaaag cauuuaauca gaguagaaaa cuggauauuu      180

agaaacccug gauuugcauu ggccgcugcu gcaauagcau gguuacuugg aagcagcaca      240

agucaaaaag uaauauaucu aguaaugaua cuauuaauag ccccagcaua uucaauuaga      300

uguauaggag ucucaaauag ggauuucgua gaaggaauga guggaggaac auggguagac      360

guaguacuag aacacggugg gugcguaaca guaauggcac aagacaaacc cacaguagau      420

auagaauuag ugacaacuac aguuaguaac auggcagaag uuagaaguua uuguuacgaa      480

gcaagcauaa gugauauggc aagcgauucu cgauguccga cucagggaga agcauaucua      540

gacaaacaau cugauacaca auaugugugu aaacgaacuc uagucgauag agggugggga      600

aauggaugug gacucuuugg aaaaggaagu cuaguaacgu gcgcuaaauu ugccuguagu      660

aagaaaauga cgggaaaauc cauacaaccc gaaaacuuag aauauagaau aauguuaagu      720

gugcauggaa gucaacauag uggaaugaua guaaacgaua caggacacga aacagaugaa      780

aauagagcaa aaguagaaau aacacccaau agccccagag cagaagcaac acuaggaggu      840

uuuggaucau uaggacucga uugugaaccu agaacugggu uagauuuuuc agauuuauau      900

uauuuaacaa ugaauaauaa acauugguua guucauaaag aaugguuuca ugauauaccu      960

uuaccauggc augcuggggc agacacagga acaccacauu ggaauaacaa agaagcauua     1020

guagaauuua aagacgcaca ugcuaaaaga caaacugugg uagugcuagg gagucaagaa     1080

ggggcaguac auacagcauu agccggagcu uuagaagcag aaauggaugg agcaaaagga     1140

agauuaucca gcggacaucu aaaauguaga uuaaaaaugg auaaauuacg uuuaaaaggu     1200

gugagcuacu cacuuuguac agcugcauuu acauucacaa aaauuccagc agaaacccua     1260

cacggaacag ucacagucga aguacaauac gcaggaacag acggaccuug caaaguaccc     1320

gcucaaaugg cagucgauau gcaaacauua acaccuguag guagacuaau uacagcaaau     1380

ccaguaauaa cagaaucaac agaaaauagu aaaaugaugc ucgaauuaga uccaccauuu     1440

ggugauucau auauaguuau aggaguagga gagaaaaaga uuacacauca uuggcauaga     1500

aguggaagua caauaggaaa agcauuugaa gcaacaguaa gaggagcaaa acgaauggca     1560

guauuaggag auacagcuug ggacuuugga aguguuggag gugcacuaaa uaguuuagga     1620

aaaggaauac aucaaauauu uggagcagca uuuaaaucau uauuuggugg uaugucgugg     1680

uuuucccaaa ucuuaauagg aacuuuauua auguggcuag gauuaaauac gaaaaaugga     1740

ucaauaucac ucaugugucu agcauuagga ggaguuuuaa ucuuuuuauc aaccgcaguu     1800

ucagcaugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10277
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10277
gggagaaagc uuaccauggg aaaaaggucc gcaggaagua uaaugugguu agcuagcuua       60

gcaguaguga uagcaugugc aggagcugca guaacauuac caucacauuc cacaagaaaa      120

cuacaaacaa gaucucaaac augguuagaa ucaagagaau acacaaaaca ccuaauacga      180

guagaaaacu ggauauuuag aaauccugga uucgcacuag ccgcagcagc aauugccugg      240

uuauuaggaa guaguacaag ccaaaaagua auauauuuag uaaugauauu auuaauugca      300

ccagcauaua guauaagaug uauaggagua aguaauagag auuuuguaga aggaaugagu      360

ggagguacau ggguagauau aguauuagaa cacggaggau guguaacagu uauggcucaa      420

gauaaaccua caguugauau ugaauuagua accacuacag uaucaaauau ggcugaagua      480

agaaguuauu guuaugaagc aaguauuagc gauauggcga gugauagcag augucccaca      540

caaggugaag cauacuuaga uaaacaaagu gacacacaau auguaugcaa aagaacucua      600

guagauagag gauggggaaa cgguugcgga uuauuuggca aagguagcuu agucacaugu      660

gcaaaauuug ccugcaguaa gaaaaugaca ggaaaaagua uacaaccaga aaauuuagaa      720

uauagaauaa ugcuuagcgu gcacggauca caacauucag gaaugauagu gaaugauaca      780

ggacaugaaa cagacgaaaa uagggcaaaa guugaaauua cgccaaauag ucccagggca      840

gaagcaaccu ugggaggauu uggcaguuua ggacuagauu gugaaccaag aaccggauua      900

gauuucucug aucuauauua uuuaacgaug aauaauaaac acugguuagu acacaaagaa      960

ugguuucaug auauaccauu accguggcac gcaggagcag auacaggaac accucauugg     1020

aauaauaaag aagcucuagu agaauuuaaa gacgcccacg cgaaaagaca aacagucgua     1080

guauuaggua gccaggaagg agccguucac acagcauuag ccggugcuuu agaagcagaa     1140

auggauggag caaaagguag acuaagcagu ggacacuuaa aauguagguu aaaaauggac     1200

aaauuaagac uaaaaggugu aucauauagu uuauguacag cggcauuuac guucacaaaa     1260

auucccgcag aaacauuaca cggaacagua acaguagaag uacaauaugc aggaaccgau     1320

ggaccaugua aaguaccagc acaaauggca guagauaugc aaacauuaac accuguagga     1380

agacuuauca cagcaaaucc uguuauaaca gaaagcacag aaaauucaaa aaugauguua     1440

gaauuagacc caccauucgg ugauaguuac auaguaauag gaguaggcga gaagaaaauu     1500

acacaucauu ggcauagauc aggauccaca auagguaaag cguucgaagc aacagugaga     1560

ggagcaaaaa gaauggcagu auuaggggau acugccuggg auuuuggauc aguaggggga     1620

gcauuaaaua gucuaggaaa agguauacac caaauuuuug gagcggcuuu uaaaucucua     1680

uuugguggga ugucaugguu uucacaaaua cuaauaggca cacuacucau guggcuagga     1740

uuaaacacga aaaauggaag cauaucauua augugucuag cacuaggagg uguccuaaua     1800

uuccugucaa cagcaguaag ugcaugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10278
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10278
gggagaaagc uuaccauggg aaaaaggagc gcaggaucaa ucaugugguu agcaucauua       60

gcaguaguaa uugccugcgc uggugcagca guaacauugc caagucauuc uacaagaaaa      120

cuacagacaa gaagccaaac auggcuagaa ucuagagaau acacaaaaca uuuaauaaga      180

guugaaaacu ggauauuuag aaauccagga uuugcacuag cagcagcagc aauagccugg      240

uuauuaggaa guucaacaag ucaaaaagua auuuacuuag uaaugauacu auuaauagcu      300

ccagcauaua guauaagaug uauaggagua ucaaauagag auuuuguuga agguaugagu      360

ggaggaacau ggguagaugu uguacuagaa cauggaggau guguaacugu aauggcacaa      420

gauaaaccaa cagucgacau ugaacuagua acaacuacag ugucuaacau ggcugaagua      480

agaucauauu gcuaugaagc aucuauaagu gauauggcau cagauagcag auguccuacu      540

caaggagaag cauaucuuga uaaacaauca gauacacaau auguauguaa aagaacauua      600

guagauagag gauggggaaa ugguuguggc uuauuuggaa aaggaagucu aguaacaugu      660

gcaaaauuug caugcaguaa aaaaaugacg ggaaaaagua uacagccaga aaaccuagaa      720

uacagaauua uguuaagcgu ucaugguagu caacauagug gaaugauagu aaacgauacu      780

gggcaugaaa cugacgagaa uagagccaaa guagaaauaa caccaaauuc accgagagca      840

gaagcuacac uugguggauu cggaucacuu ggucuagauu gcgaaccaag aacaggacuu      900

gacuucucag accuauauua ucuaacaaug aauaauaaac auugguuagu ucauaaagaa      960

ugguuucaug auauaccauu accauggcau gcaggagcag auacgggaac accacauugg     1020

aauaauaaag aagcacuagu cgaauuuaaa gaugcacaug caaaaagaca aacgguaguu     1080

guacuaggau cacaagaagg agcaguacau acagcauuag cuggggcuuu ggaagcagaa     1140

auggauggcg cgaaaggaag acuaucaagu ggacauuuaa aaugccgauu gaaaauggau     1200

aaauuaagau uaaaaggagu aaguuacuca uuauguaccg cugcguuuac auuuacgaaa     1260

auuccagcag aaacguuaca uggaacagua acagucgaag uucaauaugc aggaacagac     1320

ggaccaugua aagugccagc acaaauggca guagacaugc aaacauuaac accaguggga     1380

agauuaauaa cagcaaaccc aguaauaaca gaaucuacgg aaaauagcaa aaugauguua     1440

gaacuagacc cacccuucgg agauucauau auuguaauag gaguuggaga aaaaaaaauu     1500

acgcaucacu ggcauagaag ugguaguaca auuggaaaag cauuugaagc uacaguaagg     1560

ggagcaaaaa gaauggcagu acuaggagau acugcauggg auuucgguag uguuggaggu     1620

gcacuaaaua gccuuggaaa aggaauucau cagauauuug gagcagcauu uaaaucauua     1680

uuuggaggca ugaguugguu cagucaaaua uuaauuggaa cucuauuaau gugguuaggu     1740

uuaaacgcaa agaauggauc aauaaguuua auguguuuag cacuaggagg aguauuaaua     1800

uuccuuucaa cagccguuuc ggcuugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10279
<211>  2017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10279
gggagaaagc uuaccauggg aaaaagauca gcaggaagua uaaugugguu agcaucacua       60

gcaguaguaa uagcaugugc uggagcagca guaacauuac caucacauuc aacaagaaaa      120

uuacagacaa gaagccaaac cugguuagaa ucaagagaau acacaaaaca uuuaauaaaa      180

gucgaaaacu ggauauuuag aaauccagga uucgcucuag uagcaguagc aauagcuugg      240

uuacuaggau caagcacauc acaaaaagug aucuaucuag uuaugauacu auuaauagcc      300

ccugcauauu cuauaaggug cauaggagua agcaacagag auuuugucga aggaauguca      360

ggaggaacau ggguagaugu ugucuuagaa cauggagguu gcguuacagu aauggcacaa      420

gauaaaccaa ccguggauau ugagcuagua acgacaacag uaaguaacau ggcagaagua      480

agaaguuauu guuacgaagc aaguauauca gacauggcau cagauucaag auguccgacu      540

caaggagaag cauaucuaga uaagcaauca gauacacaau acguuugcaa aagaacauua      600

guagauagag gauggggaaa uggaugcgga uuauuuggaa aaggaaguuu aguaacaugc      660

gcaaaauuua cuuguaguaa gaaaaugaca ggaaaaagua uucaaccuga aaaucucgaa      720

uauagaauca ugcuaagugu ucauggaagc caacauucug gcaugauagg auaugaaaca      780

gaugaagaua gggcaaaagu agaaguuaca ccuaauucac caagagcaga agcaacauua      840

ggagguuuug gaaguuuggg acuagacugu gaaccucgaa cugguuuaga cuuuagugau      900

uuauacuauu uaacgaugaa uaauaaacau uggcuuguac auaaagaaug guuucaugac      960

auaccauuac cauggcacgc aggagcagau acaggaacac cacacuggaa uaauaaagaa     1020

gcauuaguag aguuuaaaga ugcucaugca aaacgacaaa cagugguagu auuaggaucu     1080

caagaaggag cuguacauac agcucuggcg ggugcauugg aagcagaaau ggauggagca     1140

aaaggacgac uguucagcgg acauuuaaaa ugcaggcuua aaauggauaa auuaagauua     1200

aaaggaguau cauauucacu auguacagcu gcuuuuacau ucacaaaagu cccagcagag     1260

acucuacaug gaacaguaac uguugaagua caauaugccg gaacagaugg accauguaaa     1320

auuccuguac aaauggcagu agauaugcaa acacuuacuc caguaggaag auuaauaacg     1380

gcaaaccccg uuauaaccga aucaacagaa aauaguaaaa ugaugcuaga acuagauccu     1440

ccauuuggug auucauauau aguaauagga guaggagaca agaaaauaac acaccauugg     1500

cauagaagug gaaguacuau aggaaaagca uuugaagcaa cagugagagg agccaaaaga     1560

auggcuguac uaggagauac agcaugggau uucgguucag uaggcggagu uuuuaacuca     1620

uuaggaaaag ggauacauca aauauuugga gcagccuuca aaucacuguu cggcggaaug     1680

agcugguuuu cucaaauauu gauugggacc cuauugguau ggcucggacu aaauacuaaa     1740

aaugguagca uaucauuaac augccuagcc uuaggaggag uaaugauuuu ccuuagcaca     1800

gcaguuagcg caugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     1860

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1980

ccccccccca aaggcucuuu ucagagccac cagaauu                              2017


<210>  10280
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10280
gggagaaagc uuaccauggg caaaagauca gcaggaagca ucaugugguu agcuaguuua       60

gcagucguaa uagccugugc aggagcagca guaacauuac cgagccacuc aacaaggaaa      120

uugcaaaccc ggagccaaac auggcuagaa aguagagaau auacaaaaca ucuaaucaga      180

gucgaaaacu ggauauucag aaauccugga uuugcauuag cagcagcagc aauagcaugg      240

cuauuaggau caaguacaag ucaaaaagua auauaucuag uuaugauauu acuaauagca      300

ccagccuacu caauaagaug uauaggagua agcaauagag acuuugucga aggaaugagc      360

ggaggaacuu ggguagaugu aguauuagaa cacggagguu gcguaacggu cauggcacaa      420

gacaaaccaa caguagacau ugaauuagua acaacgacag uuucaaauau ggcagaaguc      480

cgaaguuacu guuaugaagc gucaauaucu gacauggcaa gugacucuag auguccuacu      540

caaggagagg cuuauuuaga uaaacaaagu gauacacaau auguauguaa acgaacguua      600

guggauagag gaugggguaa ugguugcggu cuguucggua aaggaucucu aguaacaugu      660

gcaaaauuug cuuguuccaa gaaaaugaca ggaaaaucaa uacaaccaga aaauuuagaa      720

uaucgaauaa uguuaagugu acauggaagu caacauagug gaaugauugu aaaugauaca      780

ggacaugaaa cagaugaaaa uagagcaaaa guggaaauaa cuccaaauuc accaagagca      840

gaagcuacau uaggaggcuu ugguagcuua ggauuagacu gcgaaccaag gacgggucua      900

gacuuuagug accuuuauua ucuaacaaug aauaauaaac auugguuagu acauaaagaa      960

ugguuccaug auauuccauu accauggcac gcaggagcag auacuggaac accacauugg     1020

aacaauaaag aagcauuggu ugaauuuaaa gaugcacaug ccaaaagaca aacaguagua     1080

guacuuggau cccaagaagg agcaguacau acagcauuag ccggugcauu agaagcagaa     1140

auggacggag caaaagguag acuuagcagc ggacaucuua aauguagacu aaaaauggau     1200

aaauuaagau uaaaaggagu aucuuauagu cuaugcacag cagcauucac uuuuacuaaa     1260

auacccgcag aaacguuaca uggaacagua acaguagaag uacaauaugc aggaacagau     1320

ggcccaugua aaguaccagc acaaauggca guugauaugc agacauuaac accuguagga     1380

agacuaauaa cagcaaaucc aguaauaaca gaaucaacag aaaauaguaa aaugauguug     1440

gaauuagauc caccauucgg agauucuuac auaguaauag gaguaggaga aaagaaaaua     1500

acccaucauu ggcauagauc aggaaguaca auuggaaaag cauuugaagc aacaguaaga     1560

ggagcaaaac gaauggcagu acuuggagac acagcauggg auuuugguuc gguaggagga     1620

gcauuaaauu cacuagguaa aggaauacau caaauauuug gagcagcguu uaaaucacuc     1680

uucgguggaa ugucaugguu cagucaaauu cuaauaggaa cauuauuaau guggcucggu     1740

cuaaauacga aaaauggauc aauuucacuc auguguuuag ccuuaggugg aguccucaua     1800

uucuuaucaa cagcagucag cgcaugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10281
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10281
gggagaaagc uuaccauggc cauaagcggc guacccgucc ucggauucuu cauaaucgca       60

guccucauga gcgcccaaga aaguugggcc gucacccgca gaggaagugc auacuacaug      120

uaccucgacc gcaacgacgc gggagaagcc aucuccuucc caaccacccu aggcaugaac      180

aaaugcuaca uccaaaucau ggaccuagga cauaugugcg acgccacaau gagcuacgaa      240

ugccccaugc ucgacgaagg uguagaaccc gacgacguag acugcuggug caacacaaca      300

ucaaccuggg uaguauacgg aacaugccau cacaaaaagg gagaagccag acgcagccgc      360

agagccguaa cacuacccuc acacucgaca cgaaaacuuc aaacacgguc acagaccugg      420

cuagaauccc gugaauacac caaacaccuu auaagaguag agaacuggau cuuccgaaac      480

cccggguucg cccucgccgc cgccgccauc gcauggcuac ucggcuccag cacaucacaa      540

aaaguuauau accuaguuau gauccuccug auagccccug ccuauagcau aagauguaua      600

ggcguaucaa accgcgacuu cguagaaggc augucaggcg gaacaugggu ggacauaguc      660

cuagaacacg gcggcugcgu caccguaaug gcccaagaca aaccaaccgu agacauagaa      720

cuuguaacca ccacgguguc aaacauggcc gaaguacgga gcuacugcua cgaagccuca      780

auuuccgaca uggcgagcga cuccaggugc ccgacacagg gagaagcaua ccucgacaag      840

caaucagaca cccaguacgu cugcaagaga acacucguag acagaggcug gggaaacggu      900

ugcggacugu ucggcaaagg cagccugguc acaugcgcga aauucgccug uagcaaaaag      960

augaccggaa agucgaucca acccgaaaac cuagaauaca ggaucaugcu gagcguccau     1020

ggcagccaac acagcggcau gaucguaaac gacaccggac acgaaaccga cgaaaaccgg     1080

gcuaaaguag aaaucacccc aaacuccccc agagccgaag ccacgcuagg gggauucggc     1140

ucacuugggc ucgacugcga acccagaacc ggacuagauu ucagcgaucu auacuacuua     1200

accaugaaca acaaacacug gcucguacac aaagaguggu uccacgacau accccugcca     1260

uggcacgccg gcgccgacac cggcacacca cacuggaaca acaaagaagc gcuuguagag     1320

uucaaggacg cacacgcuaa acgccaaacc guaguagugc uggggagcca agaaggcgcg     1380

guacacacgg cccucgccgg ggcacuagaa gcagaaaugg acggagccaa ggggcggcug     1440

ucaagcggcc accugaaaug ccgccucaaa auggacaaac uuaggcuuaa aggagucucc     1500

uauagccuau gcacggccgc cuucaccuuc acaaaaauac cagccgaaac ccuccacggc     1560

acuguaacag ucgaggucca auacgccggc acagacggac ccuguaaagu accagcgcag     1620

auggccguag acaugcaaac gcuuacaccc guaggacgcc uaaucacagc aaacccagua     1680

aucaccgagu ccacagaaaa cagcaaaaug augcuugaac ucgauccccc auucggggac     1740

uccuacauag ucaucggcgu cggcgaaaag aaaauaaccc accacuggca ccgcuccggc     1800

ucaacgauag gaaaggcauu cgaagcaaca guaagaggag caaaaagaau ggcaguccuc     1860

ggggacacag ccugggacuu cggaucggua ggcggcgccc ucaacagccu cggaaagggc     1920

auacaccaaa ucuucggagc agccuucaaa ucacuauucg gaggcaugag cugguucagu     1980

caaauccuga ucggcacccu ccucaugugg cucggccuca acacgaaaaa cggauccauc     2040

ucccucaugu gccuagcacu cggcggaguc cucauauucc uuuccaccgc ggugagcgcg     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10282
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10282
gggagaaagc uuaccauggc aauaucggga guacccguac ugggauucuu cauaauagca       60

guccucaugu ccgcacaaga auccugggca gucacaagac gcggauccgc cuacuacaug      120

uaccuagacc gaaacgacgc cggcgaagcc aucucauucc caacaacacu aggaaugaac      180

aaaugcuaca uccaaaucau ggaccucgga cauacgugcg acgcaacaau gagcuacgaa      240

ugccccaugc uggacgaagg aguagaacca gacgacgucg acugcuggug caacacaacc      300

agcacuuggg uaguauacgg cacaugccac cacaagaaag gcgaagccag acgaagcaga      360

agggcaguaa cccuaccuuc gcacucgacc cgaaaacugc agacaagaag ccagacaugg      420

cuggaaucac gcgaauacac aaaacaccuc auacgagucg agaacuggau cuucaggaac      480

cccggauuug cccuggcggc cgccgcaauc gcauggcucc ucggcagcuc aacgucacaa      540

aaaguaauau accuggucau gauccuacuc aucgcacccg ccuacagcau cagaugcaua      600

ggagucagca accgggacuu cguagaaggc augagcggcg gcaccugggu agaugugguu      660

cuagaacacg gcgguugcgu cacagugaug gcacaagaca aacccaccgu agauauagag      720

cuagucacca ccaccgucag caauauggcc gaaguccgga gcuacugcua cgaagccagc      780

aucagcgaca uggcauccga cagccgcugc ccaacccaag gagaggcaua ccuagacaaa      840

caaagugaca cacaauacgu cugcaaacga acacucguag acagaggcug gggcaacgga      900

uguggccucu ucgguaaagg cucacucguc acaugcgcaa aauucgcaug cagcaagaaa      960

augaccggca aaagcaucca accagaaaac cuugaguaua gaaucaugcu aucgguacac     1020

ggaucccaac auucaggcau gaucguaaac gacacgggcc augaaacaga cgaaaaucga     1080

gccaaaguag aaauaacccc gaacagcccg agggcagagg caacacucgg uggauuuggc     1140

agccugggcc ucgacugcga accccgaacc ggucuugacu ucuccgaccu guacuaccua     1200

acaaugaaca acaaacacug gcucgugcau aaagaguggu uccacgacau cccacugccc     1260

uggcacgccg gcgcagacac cgguacgcca cacuggaaca acaaagaagc acucgucgag     1320

uucaaagacg cacacgcaaa acgccaaacg guaguagucc ucggcucaca agaaggagcc     1380

gugcacaccg cccuagccgg cgcacucgaa gcagaaaugg acggcgcaaa aggccgacuc     1440

uccucaggcc accucaaaug ccgccuaaaa auggacaaac ugcgccucaa aggagucagc     1500

uacuccuuau gcacagcagc cuucaccuuc acaaaaauac cugcagaaac ccuucacggc     1560

accgucaccg ucgaaguaca auacgcaggu accgacggcc ccugcaaagu accagcccaa     1620

auggccguag acaugcaaac ccugaccccc gugggcagac uuauaaccgc caaccccgua     1680

auaacagaaa gcaccgaaaa cucaaaaaug augcucgaac ucgauccccc guucggcgac     1740

agcuauauag ucaucggagu cggcgaaaag aaaaucacac aucacuggca cagaucagga     1800

agcaccaucg gaaaagccuu cgaggcgaca guacgcggag ccaaacggau ggccguccuc     1860

ggagacaccg ccugggacuu cggaucagua ggaggggcac uuaacucccu aggaaaaggc     1920

auccaccaaa ucuucggcgc agccuucaaa ucacuauucg gggguauguc cugguucucc     1980

caaauccuaa ucggaacucu ccucaugugg cucggacuca acgcgaaaaa cggcaguauc     2040

agccucaugu gccucgcacu aggaggagug cuaauauucc uaucaacagc agucagcgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10283
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10283
gggagaaagc uuaccauggc caucucaggc gucccagugc ucggauuuuu caucauagca       60

guauuaauga gcgcgcaaga aaguugggca auaaccagac gcggcuccgc cuacuacaug      120

uaccuggacc gcuccgacgc aggcaaagcc aucagcuucg caaccacccu cggaguaaau      180

aagugccacg ugcaaauaau ggaccuaggc cacaugugcg acgccacaau gucauacgaa      240

ugcccgaugc ucgacgaagg aguagaaccc gacgacgugg acugcuggug caacacaacg      300

uccacauggg uaguauacgg aaccugccac cacaagaaag gagaggcaag gcgaagccgc      360

agagcaguaa cccuacccag ccacaguaca cgaaaacuac aaacgcggag ccaaacuugg      420

cuggaaucac gcgaauacac aaaacaccua auaaaagugg aaaacuggau auuccggaac      480

cccggauucg cacugguggc aguagcgaua gccuggcugc ucggcagcuc aaccucccaa      540

aaagucaucu accucguaau gauacuccug auagcaccag cguacuccau aaggugcaua      600

ggcgucagca accgugauuu cgucgaggga augucaggag gaaccugggu agacguaguc      660

cuagaacacg ggggcugcgu gaccgucaug gcccaggaca agccuacagu cgacauagaa      720

cucgucacaa ccacgguaag caauauggca gaaguaagaa gcuacuguua cgaagcauca      780

aucagcgaca uggccagcga cucaagaugc ccaacccaag gagaagccua ccuagacaaa      840

cagagcgaca cccaauacgu cugcaagcgg acgcucguag acagaggcug gggaaaugga      900

ugcggccucu ucggaaaggg aagccuagug accugcgcca aauucacaug cagcaagaaa      960

augacaggaa aauccaucca acccgagaac cucgaauacc gcauaauguu aagcguccac     1020

gggucacaac auagcgguau gaucggcuac gaaaccgacg aagacagggc aaaaguagaa     1080

guaaccccca acagcccaag agcugaagcc acacucggag gauucggcag ccuaggacua     1140

gacugcgaac cacgcacugg acuugacuuc uccgaccuau acuaccucac gaugaacaac     1200

aagcacuggc uaguccacaa agaaugguuc cacgacauac cccuaccuug gcacgccggc     1260

gcggacaccg gaacaccaca cuggaacaac aaagaggcac ucgucgaguu caaggaugca     1320

caugccaaac gccaaacagu cguaguacug ggaagccagg aaggagccgu acacacagcc     1380

cucgcaggag cacucgaagc ugaaauggac ggcgcaaaag gccggcuuuu cagcggccac     1440

cucaaaugcc ggcuaaaaau ggacaaacuc cggcugaaag ggguaagcua cagccucugc     1500

accgcagcgu uuacguucac caaaguaccc gcagaaacac uacacggcac aguaacaguc     1560

gaggugcaau acgcaggcac cgacggcccc ugcaagauac caguacaaau ggcagucgac     1620

augcaaacac ugacaccugu cgggcgacua auaaccgcaa acccagucau caccgaaagc     1680

accgagaaca gcaaaaugau gcucgagcua gacccaccau ucggcgacag cuacaucguc     1740

auaggcgugg gcgacaagaa aaucacacac cacuggcaca gguccggcag caccaucggc     1800

aaagccuucg aagcgacagu acgcggagca aaacgaaugg cagucuuggg cgacacagcc     1860

ugggacuucg gcagcgucgg cggagucuuc aacucacuag ggaaaggaau acaccagaua     1920

uucggagccg ccuucaagag ccuauucgga ggaaugagcu gguuuagcca gauacucaua     1980

ggcacccuac ucgucuggcu aggucuaaac acaaagaacg gcuccaucuc auuaacaugc     2040

cuggcauugg gcggagugau gauauuucuc agcacagccg uaagcgcgug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10284
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10284
gggagaaagc uuaccauggc cauauccggc guaccagucc ucggguucuu cauuauagca       60

guccucauga gcgcacaaga aucgugggcc guaaccagac gaggaucugc auauuacaug      120

uaccuagacc ggaacgacgc gggagaagcc aucucauuuc ccacaacacu cggaaugaau      180

aaaugcuaca uccaaaucau ggaccucggc cacaugugcg acgcaacgau gagcuacgaa      240

ugccccaugc uagacgaggg ggucgaaccc gacgacgucg acugcuggug caacacaaca      300

ucaaccuggg uaguauacgg cacaugccac cacaaaaagg gcgaagcccg acgauccagg      360

cgagcaguaa cauuacccuc ccauuccacc cgcaaacucc aaacgcgcuc gcaaaccugg      420

cuagaaucac gagaauacac aaaacaccuc auccgaguag aaaacuggau cuucagaaau      480

ccgggauucg cccuggccgc ugccgccauc gccuggcuac ucggcuccag caccucacag      540

aaagucaucu accucguuau gauacuccuc auagcgccag ccuacucuau ccgcugcauc      600

ggaguaagca accgagauuu cguagaaggc augucaggcg gcacaugggu cgacgucguc      660

cuagaacacg gaggcugcgu aacagucaug gcccaagaca aaccgaccgu cgacaucgag      720

cuaguaacca caaccgucuc caauauggcc gaaguccgaa gcuacugcua cgaagcguca      780

aucuccgaca uggcaagcga cucccgaugc ccaacccaag gcgaagccua ccuagacaaa      840

caguccgaca cccaauacgu uugcaaacgc acacucgucg accgaggaug gggcaacggc      900

ugcggccucu ucgggaaagg aagccucgua accugcgcaa aauucgcaug cagcaagaaa      960

augaccggaa aauccaucca acccgaaaac cuggaauacc gcauaaugcu cucaguacac     1020

ggaucccagc acagcggaau gauagugaac gauacaggac acgaaacuga cgaaaaccga     1080

gcaaaaguag aaaucacccc caacucacca cgagcagaag ccacccuagg cggauucggc     1140

ucauuaggcc ucgacugcga accuaggacg ggccucgacu ucuccgaccu auacuaccug     1200

acaaugaaca acaaacacug gcuaguacac aaagaauggu uucacgauau cccccuaccc     1260

uggcacgccg gcgccgacac aggcacaccg cacuggaaca acaaggaagc cuuaguagag     1320

uucaaagacg cccacgccaa gcgccaaacc gucguggucc ugggauccca agaaggcgcc     1380

guacacaccg cccuagcagg cgcccuugag gccgaaaugg acggggcaaa aggccgccua     1440

ucauccggac aucucaagug ccggcucaaa auggacaaac uacgacuaaa aggcguaagc     1500

uacucccucu gcacagccgc uuucacauuc accaaaauac cagcggaaac ucuccacggc     1560

accgucaccg ucgaagugca auacgccgga acagacggac caugcaaagu gcccgcccaa     1620

auggccgucg acaugcagac ucuaacccca guagggcgac ucauaaccgc aaaucccguc     1680

aucaccgaaa gcaccgagaa cagcaaaaug augcucgaac uggacccucc cuucggcgac     1740

agcuacaucg ucaucggagu aggcgaaaag aaaaucaccc accacuggca ccgaucggga     1800

agcaccaucg gcaaagccuu cgaagcgaca gucagaggcg caaaacgcau ggccguccuc     1860

ggagacaccg caugggacuu cggcagcguc ggaggcgcac ucaacucucu gggcaaaggg     1920

auccaccaaa uauucggcgc cgccuucaaa ucccucuucg ggggaauguc gugguucuca     1980

cagauacuua uaggaacacu acucaugugg cucggacuaa acaccaaaaa cggcuccaua     2040

ucccucaugu gccuagcccu aggaggaguc cuaaucuucc ucucgacagc ggucuccgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10285
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10285
gggagaaagc uuaccaugga cuggacaugg auacucuucc uaguagccgc cgccacccgc       60

guacacucag ugacgcgccg cggcucagca uacuacaugu accucgaccg gaacgacgcc      120

ggagaggcga uaucauuccc caccacacua ggaaugaaca aaugcuacau acagaucaug      180

gaccucggcc acaugugcga cgccacaaug uccuacgagu gcccaaugcu ggacgaaggg      240

guagaaccag acgacgucga cugcuggugc aacaccacgu ccacaugggu ggucuacggc      300

acaugccacc acaagaaagg agaagcgcgc cgaucacgca gagccgucac ccucccaagc      360

cacuccacaa gaaaacucca aaccagaagc caaacuuggc ucgaaucccg ggaauacacc      420

aagcaccuca uacgcguaga gaacuggaua uuccgcaacc caggcuucgc gcuagcagcc      480

gccgccauag ccuggcuacu aggcucaucc acaucacaaa aagugaucua ucuaguaaug      540

auacuacuaa uagcacccgc cuacagcauc agaugcauag gcgucucaaa cagagacuuc      600

guagaaggca ugucaggagg aaccugggua gauaucguac ucgaacacgg cggaugugua      660

accgucaugg cccaagacaa acccacugua gauaucgagc uaguaacaac aacaguaucc      720

aacauggccg aaguaagauc cuacugcuac gaagcaucaa ucucagacau ggccucagac      780

ucgaggugcc cuacucaggg agaagccuac cuagacaaac aaagcgacac gcaauacguc      840

ugcaaaagaa cccuagucga uagaggcugg ggaaacgggu gcgggcuauu cggcaaggga      900

ucacucguaa ccugcgcaaa guucgcaugc uccaagaaaa ugaccgguaa aagcauccaa      960

cccgaaaacc ucgaauacag aaucaugcua ucgguacacg gaucccagca cuccggaaug     1020

auuguuaacg acacggguca cgaaacagac gaaaaccggg ccaaggucga aauaacacca     1080

aacagcccac gagcagaagc cacccuagga ggcuucggcu cccucggccu cgacugcgaa     1140

ccccguaccg gcuuggacuu cagcgaccua uacuaccuca ccaugaacaa caaacacugg     1200

cugguccaca aagaaugguu ccacgacauu ccccuccccu ggcacgcagg ggccgacaca     1260

ggaaccccac acuggaacaa caaagaggcc cuuguagagu ucaaagacgc acacgcaaaa     1320

cgccaaacag ucgucguccu cggcucgcaa gaaggagccg uacacacagc acuagccggc     1380

gcacuagaag cagaaaugga cggcgcaaaa ggccgccuaa gcucaggcca ccucaagugc     1440

cggcucaaga uggacaaacu acgccuaaaa ggagucucau acucccuaug caccgcggcc     1500

uucaccuuca ccaaaauccc ggcagagacc cuacacggaa ccguaaccgu ugaaguucaa     1560

uaugccggaa ccgacggacc cugcaaagua cccgcccaga uggcagucga caugcaaaca     1620

cuuaccccag uuggccgccu caucacggca aaucccguca ucaccgaaag uacagaaaac     1680

ucaaaaauga ugcuagaacu agacccgccc uuuggcgacu cauacauagu cauaggggua     1740

ggagagaaaa agauaaccca ccacuggcac cgcagcgggu ccacgauagg uaaagccuuc     1800

gaagcaaccg uaagaggcgc gaaacgaaug gcaguacuag gugacaccgc augggacuuc     1860

gggagcgucg gcggggcacu aaauucacuu gggaagggaa uccaccaaau cuucggggcc     1920

gcauucaaga gucucuucgg cggcaugagc ugguucucac aaauccucau aggaacccuc     1980

cuaauguggc uaggccucaa caccaaaaac ggaagcauau cccuaaugug ccuagcacuc     2040

ggaggcguac ucaucuuccu cagcacagcg guaagcgcgu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10286
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10286
gggagaaagc uuaccaugga cuggacgugg auacuauucc ucguagccgc cgcaacccgc       60

guccacagcg uaacccgaag aggcuccgcu uacuacaugu accucgaccg aaacgacgcc      120

ggcgaagcca uaucauuccc caccacccuc ggcaugaaca aaugcuacau ccaaauuaug      180

gaccuagggc acacuugcga cgccacaaug agcuacgagu gccccaugcu cgacgaaggc      240

gucgaaccag acgacgugga cugcuggugc aacaccacca gcacaugggu aguauacgga      300

accugccacc acaagaaagg cgaagcccga agaucacgcc gggccguuac ccuccccuca      360

cacuccacac gaaagcucca aacccgcucc caaaccuggc ucgaaucucg agaauacaca      420

aagcaccuua uaagaguuga gaacuggauc uucagaaacc ccggcuucgc ccuagccgca      480

gccgcaaucg ccuggcuccu cggcuccuca acaucccaaa aagucauaua ccuugucaug      540

auccuccuca uagcaccagc cuacucgauc cgaugcaucg gcguuuccaa ccgcgacuuu      600

gucgaaggca ugaguggagg caccuggguc gacguaguac uagaacacgg aggcugcguc      660

acaguaaugg cacaagacaa accgacagua gacaucgagc uaguuacaac aaccguaucu      720

aauauggccg aaguccgcuc auacugcuac gaagccucca ucuccgacau ggcauccgac      780

uccagaugcc cuacccaagg cgaagccuac cucgacaaac aauccgacac ccaauacguc      840

ugcaaacgca cccucguaga ccgagggugg ggcaacgguu gcggccuguu cggaaaggga      900

ucccuaguca caugcgcaaa guuugccugc uccaagaaaa ugacgggaaa aucaauacaa      960

cccgaaaacc uagaauaccg cauaaugcuc agcguccacg ggucacaaca cagcgguaug     1020

auagugaacg acacuggaca cgaaacagac gagaacagag cgaaagucga aaucacaccg     1080

aacuccccuc gcgcagaagc cacgcuagga ggguucggau cacuuggacu cgacugcgaa     1140

ccccgcaccg gccucgacuu cagcgacuua uacuaccuaa caaugaacaa caaacauugg     1200

cuaguccaca aagaaugguu ccacgacaua ccccuacccu ggcacgccgg cgcagacaca     1260

ggaacacccc acuggaacaa uaaagaagcc cucgucgagu ucaaagacgc ccacgcgaaa     1320

agacaaaccg ucguuguacu gggcagccaa gaaggcgccg uccacaccgc acuagcgggg     1380

gcucuagaag cagaaaugga uggcgccaag gggcgacuau caucaggcca ccucaaaugc     1440

agacuaaaaa uggacaaacu ccgccucaaa ggaguaucau acucacuaug caccgccgcg     1500

uucacauuca ccaaaauacc cgcugaaacu cuccacggaa cagucaccgu cgaaguccaa     1560

uacgcaggaa ccgacggccc cugcaaggua ccagcccaaa uggccguaga caugcaaaca     1620

cuaacccccg ucggaagacu aaucaccgcg aaccccguaa uaacagaauc cacagagaac     1680

ucaaaaauga ugcucgaacu agacccaccg uucggagacu cuuacauagu cauaggaguc     1740

ggagagaaaa agaucacaca ccacuggcac cgcucaggcu caacaauugg caaagccuuc     1800

gaagccacgg uacggggagc caaaagaaug gccguacuag gggacacagc cugggacuuc     1860

ggaucagucg gcggugcacu aaauucacua ggaaaaggca uccaccagau cuucggcgca     1920

gccuucaaaa gccuauuugg uggaauguca ugguucagcc aaauccucau agggacccuc     1980

cucauguggc ucggccucaa cgcuaaaaac ggaagcaucu cgcuaaugug ccuagcccuc     2040

gggggcgucc uaauauuccu cagcacggca gucagcgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10287
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10287
gggagaaagc uuaccaugga cuggaccugg auacuguucu uaguagccgc cgccacgcga       60

guccacagca uaacacgcag gggaucagca uacuacaugu accucgaccg auccgacgcc      120

ggaaaagcaa uaucauucgc aaccacgcua ggagucaaca aaugccacgu gcaaaucaug      180

gaccuagguc acaugugcga cgccaccaug uccuacgagu gcccuaugcu cgacgaagga      240

guagaaccag augacgucga cugcuggugu aacacaaccu ccacaugggu cgucuacggc      300

acaugccacc acaagaaagg agaagccaga cgaagccguc gcgccgucac acuaccaagc      360

cacagcacaa gaaaacucca aacccgguca caaacauggc uugaaagccg agaauauaca      420

aaacaccuca uaaaaguaga aaacuggauc uuccggaacc caggguucgc gcucguagcc      480

guggcaauag ccuggcuccu cggauccagc acaucccaaa aagucaucua ccuaguaaug      540

auccucuuga uagcacccgc cuacucaaua cgaugcauag gagucagcaa ccgcgauuuc      600

guagaaggaa uguccggcgg aacauggguc gacgucgucc ucgaacacgg uggaugcguc      660

accgucaugg cacaagacaa acccacaguc gacauagagc uagucaccac aacuguaagc      720

aacauggccg aaguacgcuc cuacugcuau gaagcaucca uauccgacau ggcaucggac      780

agccgcugcc caacgcaagg cgaagccuac cuagacaaac aaucagauac ccaguacguc      840

ugcaaaagga cccuaguaga ccgaggaugg ggcaacggau gcggacucuu cggaaaaggc      900

agccuaguaa ccugcgcaaa auucacaugc uccaagaaaa ugacaggaaa aagcauacaa      960

ccggaaaacc uagaguacag gaucaugcua agcgugcacg gcucccaaca cucaggaaug     1020

auaggcuacg aaaccgacga agaccgagca aaagucgaag uaacacccaa cucaccccgg     1080

gcagaagcaa cccuaggggg auucggaucc cuaggacucg acugcgaacc ccgcacagga     1140

cuggacuucu ccgaccuaua cuaccuaacg augaacaaca aacacugguu aguccacaaa     1200

gagugguucc acgauauccc ccuaccaugg cacgccggag ccgacacagg aacgccacac     1260

uggaacaaca aagaagcccu agucgaguuc aaagacgcac acgccaaaag gcagacaguc     1320

guagugcuag gcucgcaaga aggggcaguc cacaccgcac ucgccggagc acuagaagcc     1380

gaaauggacg gagccaaggg gcggcuauuc ucaggccacc ugaaauguag acuuaagaug     1440

gauaaacugc gacuaaaagg agugagcuac ucccucugca cagccgcauu cacguucaca     1500

aaaguaccgg ccgaaacacu gcacgggaca gucaccguug aaguccaaua cgcagggacu     1560

gacggccccu gcaaaauccc cguccaaaug gccgucgaca ugcaaacccu aacccccgua     1620

ggacgacuaa ucacggcaaa cccuguaaua acagaaucaa cagaaaacag caaaaugaug     1680

cuugaacuag acccaccauu cggagacucc uacaucguaa ucggaguagg ugacaagaag     1740

auaacacacc acuggcacag aagcggcucc acaaucggga aagcguucga agccacaguc     1800

cgcggcgcca aaagaauggc uguacuagga gacaccgcau gggacuucgg cuccguaggc     1860

ggagucuuca acucgcuagg gaaaggaaua caccaaauau ucggagcagc cuucaaaucc     1920

cuauucggag gaauguccug guucucgcaa auccuaaucg gcacacuccu ggucuggcua     1980

ggacuaaaca cgaaaaaugg aagcauuucc cuaacaugcc ucgcccucgg aggaguaaug     2040

auauuccuca gcaccgccgu aagcgccuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10288
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10288
gggagaaagc uuaccaugga cuggaccugg auacuauucc ucguagccgc cgcaacacga       60

guccacagcg uaaccaggcg cggaucagcc uacuacaugu accuggaccg aaacgaugcc      120

ggcgaggcga uaucauuccc cacaacccuc ggcaugaaca aaugcuacau ccagaucaug      180

gaccucgggc auaugugcga cgccaccaug uccuaugaau gcccgaugcu cgaugaagga      240

gucgaaccag acgacgucga cugcuggugc aacaccacca gcaccugggu cguauacgga      300

accugccacc acaagaaagg cgaagcccgc cgaagcagac gcgcagucac acuaccaagc      360

cacucaacac gaaaacuaca aacccgauca caaaccuggc ucgaaucccg agaauacaca      420

aaacaccuaa uccgagucga aaacuggaua uuccgaaacc caggauucgc ccucgcggcc      480

gccgccauag ccuggcuacu gggauccagc acaagccaaa aagucaucua ccuugucaug      540

auccuacuaa ucgccccagc cuacuccaua cgaugcaucg gagucagcaa cagagacuuc      600

gucgaaggca ugucaggagg aacaugggua gacguaguac ucgaacacgg cggcugcgua      660

accguaaugg cccaagacaa acccacagua gacauagaac uuguaaccac cacagucucc      720

aacauggcag aaguccgcuc auacugcuac gaagcaucca ucagcgacau ggcaagcgac      780

ucacgcugcc caacccaagg cgaagcauac cuggacaaac aaagcgacac gcaauacguc      840

ugcaaaagaa cacucgugga ccgcggaugg ggcaauggau gcggccucuu cggcaagggg      900

agccuaguga ccugcgcaaa auucgccugu ucaaagaaaa ugaccggcaa aagcauacaa      960

ccugagaacc ucgaauaccg cauuaugcug agcguccacg ggagccaaca cagcggcaug     1020

aucguaaacg acaccgguca cgaaaccgac gaaaaccgcg ccaaagucga gaucacccca     1080

aacucacccc gcgccgaggc uacacuggga ggauucggcu cacuagggcu ugacugcgaa     1140

ccgagaacag gcuuagacuu uucagaccuu uacuacuuaa ccaugaacaa caaacacugg     1200

cucguccaca aagaaugguu ucacgacauc ccccugccau ggcacgccgg cgcugacacc     1260

gguacaccac acuggaacaa caaagaggcc cuaguagagu ucaaagacgc ccacgcaaaa     1320

cgccaaaccg uagucguacu aggcucccaa gaaggagcag uacacaccgc ccuggccgga     1380

gcacuagaag cagagaugga cggagccaag gggcgccuca gcuccgggca ccucaaaugu     1440

cgccugaaaa uggacaagcu aaggcuaaag ggcguauccu acucccuaug cacagccgcc     1500

uucaccuuca ccaaaauccc agcagaaacc cuccacggaa cggugacugu ggaaguccaa     1560

uacgcaggaa ccgauggacc cugcaaagua cccgcacaga uggcggugga caugcaaacc     1620

cuaacgcccg ucggacgccu cauaaccgcc aacccaguca uaacagaauc aacagaaaac     1680

agcaaaauga ugcucgagcu cgacccaccc uucggagaca gcuacaucgu caucggggua     1740

ggcgaaaaga aaauaaccca ccacuggcac agaucaggau cuacaaucgg aaaagccuuc     1800

gaagccaccg uccgcggugc gaaacgaaug gccguccuag gcgacaccgc cugggacuuc     1860

ggcagcgucg ggggcgcacu caacucccua ggaaaaggca uccaccaaau cuucggcgcc     1920

gccuucaaaa gccucuucgg aggcauguca ugguucagcc agauccucau aggcacacuc     1980

cuaauguggc uaggccucaa caccaaaaac gggucaauua gccuaaugug ccucgcacug     2040

ggaggcgugc uaaucuuccu aagcaccgca gucucggcau gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10289
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10289
gggagaaagc uuaccauggg aaaaagaagc gcaggaucaa uaauguggcu agcuucccug       60

gccguaguaa ucgccugcgc aggcgcagua acacgucgag gguccgcaua cuacauguac      120

cucgauagga acgacgcagg agaggcaauc ucuuucccca ccacacuagg caugaacaaa      180

ugcuacaucc aaaucaugga ccuaggacac augugcgacg ccacuauguc cuacgaaugc      240

cccaugcuag acgaaggagu agaaccggac gacguugacu gcuggugcaa caccacauca      300

accugggucg ucuacggaac augccaccac aagaaaggag aagccagacg aagcagacga      360

gccguuacac uaccauccca cagcacaaga aaacuacaaa ccagaucaca gaccuggcua      420

gaaagcagag aauacaccaa acaucuaauc cgagucgaaa acuggaucuu cagaaacccc      480

ggcuucgccc uggcagcagc cgcaauagcc uggcuccucg gcagcaguac aucccaaaaa      540

gugauauauc ucgucaugau acuauugauc gcacccgcau acagcaucag auguauaggc      600

gucagcaacc gugacuucgu agagggaaug agcggcggga ccugggucga caucguccug      660

gaacacggcg gaugcguaac cguuauggca caggacaaac ccaccguaga caucgagcua      720

guaacaacca cagucuccaa cauggccgaa guacgcuccu acugcuacga agcguccaua      780

agcgacaugg caagcgacag ccgcugccca acccaagggg aggcauaccu agacaaacaa      840

agcgacacuc aguacgucug uaaaagaaca cucguagaca gaggaugggg caauggaugc      900

ggccuauucg gaaaaggcag ccucguaacc ugcgccaaau ucgcaugcag uaagaaaaug      960

accggaaaaa gcauacaacc cgagaaucua gaguaccgga ucaugcucag cguacacggc     1020

ucccaacacu ccggaaugau aguaaacgac acaggacacg aaacagacga aaaccgggcc     1080

aaagucgaga ucacaccaaa cuccccaagg gccgaagcca cccugggcgg uuucggcagc     1140

cuaggacuag acugcgaacc ccgcaccggc cucgacuuca gcgaccuaua cuaccuaacc     1200

augaacaaca aacacugguu aguccacaaa gaaugguucc acgacauacc ccucccgugg     1260

cacgcaggcg cagacaccgg caccccucac uggaacaaca aagaagcacu cgucgaguuc     1320

aaagacgccc acgccaaaag gcaaaccguc gucguacucg ggucacaaga aggagccgua     1380

cacaccgcac uagccggagc ccuagaagca gaaauggacg gcgcaaaagg gagacuaucc     1440

uccggccacc ucaaaugccg acugaaaaug gauaaacuac gacuaaaagg cgucagcuac     1500

agccugugca ccgcagcauu caccuucacc aagauacccg ccgaaacccu ccacggaaca     1560

gucaccgucg aaguccaaua ugcaggaaca gacggccccu gcaaaguccc cgcacaaaug     1620

gccguggaca ugcaaacccu uacaccagua ggaagacuca ucacagcaaa cccggucauc     1680

accgaaagca cggaaaacuc gaagaugaug cucgagcuag acccgcccuu cggagauagc     1740

uauauaguaa uaggaguugg agaaaagaaa auaacccacc acuggcacag aagcgggucc     1800

acaaucggaa aagcauucga agcgacagua cgaggggcca aacgaauggc aguccucgga     1860

gacacagccu gggacuucgg auccguagga ggagcccuaa acucgcucgg caaaggcaua     1920

caccaaaucu ucggcgccgc guucaaaucc cuauucggag gcaugagcug guucagccaa     1980

auacuaauag guacccuacu cauguggcuc ggacugaaca ccaaaaacgg aagcaucagu     2040

cuuaugugcc uagcacucgg aggcguacua auauuccuau caacagccgu aagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10290
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10290
gggagaaagc uuaccauggg caaaaggucc gccggaagca uaauguggcu ggccagccua       60

gcagucguga ucgcaugcgc cggcgcggua acacgccggg gcucggcaua cuacauguac      120

cucgaccgaa acgacgccgg ggaagccauc agcuucccga ccacucucgg gaugaacaag      180

ugcuacauac agauaaugga ccuuggacau accugcgacg caacaaugag cuacgaaugc      240

ccaauguuag acgagggagu agaaccagac gacguggacu gcuggugcaa cacaacgucc      300

accuggguag ucuacggaac cugccaccac aagaaaggcg aggcgcgaag aagccgccga      360

gccgucacac uacccaguca cucaacacgg aaacuacaaa caagaucaca aacauggcua      420

gagagcaggg aauacaccaa acaccucauc cgagucgaga acuggauauu cagaaaccca      480

ggcuucgccc ucgccgccgc ggcgauagcc uggcuacucg ggagcuccac aagccagaaa      540

guaauauacc uagugaugau acuccuaaua gcgccugcau acucgauccg augcauugga      600

guaagcaacc gcgacuucgu ggaaggcaug ucaggcggaa ccuggguaga cguaguccua      660

gagcacggag gcugcguaac cguaauggcc caggacaaac ccacagucga caucgagcua      720

gucacgacaa ccgucagcaa cauggcagaa guacguagcu acugcuacga agcaucaauc      780

ucagacaugg caucagacag ucgaugcccc acacaaggag aagccuaccu agacaaacag      840

ucagacacac aauacgucug caaaaggacc cucgucgacc gaggcugggg gaacgggugc      900

ggauuguucg gcaaaggauc ccucguuaca ugcgcaaaau uugcauguuc gaagaaaaug      960

acugggaaau caauacaacc ggaaaaucug gaauacagaa ucaugcuauc cguacacggc     1020

agccagcaca gcgguaugau cgucaaugac accggccacg aaacugacga aaacagagca     1080

aaagucgaaa ucacgcccaa cagcccacgg gcggaggcca cacucggagg cuucggauca     1140

cuaggccuag acugcgaacc acgcaccggc cuagacuuca gugaccuaua uuaccugacc     1200

augaacaaca aacacuggcu cguacacaaa gaaugguucc acgacauacc ccuaccgugg     1260

cacgcggggg ccgacaccgg gacaccacac uggaacaaua aagaagcccu aguagaguuc     1320

aaagacgcgc acgccaaacg ccaaacaguc guaguacucg gcucacaaga aggagccguc     1380

cacaccgcac ucgcaggagc ucucgaagca gaaauggacg gagccaaagg acgacuuagc     1440

agcggacacc uaaaaugcag acucaaaaug gacaaacuca gauuaaaagg agucagcuac     1500

ucgcuaugca cagcagcauu caccuucaca aagauccccg cggaaacccu acacgggacg     1560

gucacaguag aaguccaaua cgccggcacg gacgggccau gcaagguucc cgcacaaaug     1620

gcggucgaca ugcagacacu gacaccaguc ggcagacuaa uaaccgcgaa ccccguaaua     1680

acagaaagca cagagaacuc gaaaaugaug cuggaacuag acccaccguu cggagacuca     1740

uauauuguga ucggaguagg agaaaagaaa auaacacacc acuggcaucg cagcgggagc     1800

acaaucggaa aagcauucga agcuacaguc cgcggcgcca aaagaauggc agugcucggc     1860

gacacggccu gggacuucgg aucaguaggc ggagcccuaa acucacuugg aaaaggaaua     1920

caccaaauau ucggcgcagc guucaaaagu cucuucggcg ggaugucaug guucagccaa     1980

auccucaucg gcacccuacu aauguggcuc ggccuaaaug caaagaacgg cagcauaagc     2040

cucaugugcc uagcccuagg aggaguacua aucuuccuaa guaccgccgu aagcgcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10291
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10291
gggagaaagc uuaccauggg caagcgauca gccggaucca ucauguggcu cgccagccua       60

gcaguaguca ucgcaugcgc aggggccauc acaagacgag gcuccgcgua uuacauguac      120

cuagaccgua gcgacgccgg aaaggcaauc agcuucgcaa ccacccuggg agucaacaaa      180

ugucacguac aaaucaugga ccuaggacau augugcgacg caaccaugag cuacgaaugc      240

cccaugcuag acgaaggcgu cgaacccgac gacguagacu gcuggugcaa cacaacuuca      300

acaugggucg ucuacggaac augccaccac aaaaagggcg aagcaagacg cucccguaga      360

gccgugaccc ugccaagcca cucaaccaga aaacugcaaa caagaucaca aacauggcua      420

gaaagccgcg aguacacgaa acaccucaua aaaguagaaa acuggaucuu ccgcaacccc      480

ggguucgcac ucguagccgu cgcaauagcc uggcugcucg gcuccagcac aucccagaag      540

guaauauacc uagucaugau ccugcucauc gcgccagcau acuccauacg augcaucggc      600

gucagcaaca gagauuucgu agagggaaug agcggcggca ccuggguaga ugucguccua      660

gaacacggag gcugcgucac cguaauggca caggacaagc ccacaguaga cauagagcua      720

gucacgacaa cagucagcaa cauggcagaa guaagaagcu acugcuauga agcaucuauc      780

agcgacaugg cgagcgacag ccgaugcccc acgcaaggcg aggccuaccu cgacaagcag      840

uccgacacuc aauacguaug caaacgaacc cucguagacc gggguugggg aaacggaugc      900

gggcuauucg gaaaaggcuc ccuaguaacc ugcgccaaau uuaccugcuc caagaaaaug      960

accggaaaau cgauccaacc agaaaaccug gaauauagaa uaaugcucag cguccacggc     1020

agccaacauu cuggcaugau aggcuacgaa accgacgagg acagggcaaa agucgaggua     1080

acgccaaaca gcccaagggc agaagcgacc cucggaggau ucggcagccu cggacucgau     1140

ugcgaacccc gcacaggccu cgacuucuca gaccucuacu accucacaau gaacaacaaa     1200

cacuggcucg uacacaagga augguuccac gacauaccac ugcccuggca ugccggcgcu     1260

gauacaggaa caccacacug gaacaacaaa gaagcccucg uggaguucaa agacgcccac     1320

gcaaaacgcc aaaccguagu aguccuagga agccaagaag gcgcgguaca cacagcccuc     1380

gccggagccc uagaagcaga aauggacgga gccaaaggua gacuauucuc uggacaccuc     1440

aaaugccgau uaaaaaugga caaacuccga cuaaagggug uaucauauag ccuaugcaca     1500

gcagccuuca cauucacaaa ggugccagca gaaacgcuuc acgggaccgu caccgucgaa     1560

guacaguacg ccggcacaga cggaccgugc aaaauaccag uccagauggc aguagacaug     1620

caaacacuca caccggucgg caggcuaaua acagccaacc caguaauaac agaaagcaca     1680

gagaacagca agaugaugcu agaguuagac ccgcccuucg gggacagcua cauaguaaua     1740

ggcgucggag acaagaaaau aacacaccac uggcaccgau caggcagcac gaucggaaaa     1800

gccuucgaag caaccguaag gggcgccaaa agaauggcag uacuuggaga cacagcaugg     1860

gacuucggaa gcgucggcgg aguauucaau aguuuaggca aaggcauaca ccagauauuc     1920

ggagccgccu ucaaaucacu auucggaggg augucauggu ucagccaaau ccucauaggc     1980

acccuacucg uauggcuagg ccucaacacc aaaaauggcu ccauaagucu gacaugccuc     2040

gccuuaggcg gcguaaugau auuccuauca accgccguaa gcgccugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10292
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10292
gggagaaagc uuaccauggg aaaacgcuca gcgggaucaa uaauguggcu cgccucccua       60

gcagucguca ucgccugcgc cggagccguc acucgccgcg gcuccgcaua cuacauguac      120

cucgaccgaa acgacgccgg agaagccaua ucauucccca caacccucgg aaugaacaaa      180

ugcuacaucc aaaucaugga ccuaggccau augugcgacg caacgauguc auacgaaugc      240

cccaugcucg acgaaggagu agaacccgac gacguagacu gcuggugcaa cacaacgagc      300

accugggucg ucuacgguac cugccaccac aagaaaggcg aagcccgcag auccagacga      360

gcaguaacuc ucccauccca cagcacccgc aaacuacaga caaggucgca aaccuggcuc      420

gaaagcagag aauacacaaa acaccucauc cgcguagaga acuggaucuu ccgaaauccc      480

gguuucgccc uagcagccgc agcaauagca uggcuccucg gcucaagcac cagucaaaaa      540

guaauauacc uagucaugau acuccuaaua gcacccgcau acagcauaag augcaucggc      600

guaucgaacc gcgacuucgu cgagggaaug uccggaggaa cguggguuga cguaguacua      660

gagcacggcg ggugcguaac uguaauggcc caagacaaac ccacaguuga cauagagcuc      720

gucaccacaa cggucucaaa cauggccgaa guccggaguu acugcuauga agcgagcauc      780

agcgacaugg cauccgacuc ccgaugcccc acacaaggcg aagccuaccu agacaaacag      840

ucagacacac aauacgucug caaacggaca cucgucgaca gaggaugggg caacggcugc      900

ggccucuucg gcaaaggcag ccuugucacc ugugcgaaau uugccugcag caagaaaaug      960

accggcaaau ccauacaacc cgaaaaccug gaauaccgaa ucaugcuguc aguccacgga     1020

ucccaacaca gcggaaugau cguuaacgac acaggucacg aaaccgacga aaauagagcc     1080

aaaguagaaa uaacaccgaa cagcccccga gccgaagcca cacuaggagg uuuuggcucc     1140

cucggccucg acugcgagcc acgcaccgga cucgauuucu cagaccucua cuacuuaacg     1200

augaacaaca aacacuggcu cguccacaaa gaaugguucc acgacauccc ccucccaugg     1260

cacgcaggag cagacacagg cacaccacac uggaacaaca aggaagcccu agucgaguuc     1320

aaagacgccc augccaaacg ccaaaccgua gucgugcucg guucacaaga aggcgccguc     1380

cacacggcac ucgccggagc acucgaagcc gaaauggacg gcgcaaaggg acgacuaucc     1440

ucaggccacc ucaaaugcag acucaaaaug gacaaacuaa ggcuaaaagg cguaagcuac     1500

ucccuaugua cagcagcauu cacauucacc aaaaucccag cagaaacacu ucacggcaca     1560

gucacaguag aaguccaaua cgccgggaca gacgggccau gcaaagugcc ggcacagaug     1620

gccguagaca ugcaaacccu aacccccgua ggccgucuca ucaccgccaa cccaguaauc     1680

accgaaagca ccgaaaacuc caaaaugaug cuagaacucg acccacccuu cggggacagu     1740

uacaucguaa ucggaguagg ggaaaagaaa aucacccacc acuggcaccg auccggcucc     1800

accaucggga aggcuuucga agcaacggua cgcggcgcaa aacggauggc gguacucgga     1860

gacacagccu gggacuucgg cagcgucggc ggagcccuga acucacuagg gaaaggaaua     1920

caccaaaucu ucggcgccgc cuucaaaucg cuauucgggg gcaugagcug guucucacaa     1980

aucuuaaucg gaacccuacu caugugguug ggccugaaca ccaagaacgg auccaucuca     2040

cugauguguc uagcucucgg cgggguacuc aucuuccuga gcaccgcagu cagcgccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10293
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10293
gggagaaagc uuaccauggc caucucaggc guaccagucc uaggguucuu caucaucgcc       60

guccucauga gcgcccaaga auccugggcc guaacccgcc gaggauccgc cuacuacaug      120

uaccuagaca gaaacgacgc aggagaagcc aucuccuucc cgacaacacu cggaaugaac      180

aaaugcuaca uacaaaucau ggaccucggc cacaugugcg acgcaacaau gagcuacgaa      240

ugucccaugc uagacgaagg agucgaaccc gacgacgucg acugcuggug caacacuacc      300

agcaccuggg uggucuacgg aaccugccac cacaaaaagg gcgaagcacg acgaaguaga      360

cgcgccguca cccuccccag ccacucaaca cguaaacuac aaacccgaag ccagaccugg      420

cucgaaagcc gagaauacac caaacaccuc aucagaguag aaaauuggau cuucagaaac      480

cccggguucg cacuggcggc agccgccauc gccuggcuac ucggaucuag cacgucccaa      540

aaggucauau accuggucau gauccuacuu aucgccccag cauacuccau ccgcuguaua      600

ggcguaucca accgugacuu ugucgaaggc auguccggcg gaaccugggu cgacauaguc      660

cucgaacacg gcggaugugu caccguaaug gcacaggaca aaccaacagu cgacaucgag      720

cuaguaacca ccaccgucuc caauauggca gaaguaagau ccuacugcua cgaagccuca      780

aucagcgaca uggccucaga cagccgaugc cccacacaag gcgaagcaua ccuagauaag      840

cagucagaca cccaguacgu cugcaagcgc acccuagucg accgcggaug gggaaacggc      900

ugcgggcuau ucggaaaagg aagccuagua accugcgcca aguucgcuug uucaaagaaa      960

augaccggaa aauccauaca accagaaaac cucgaauacc gcaucaugcu auccguacac     1020

ggcucacaac acagcggcau gauaguaaac gacaccggac acgaaaccga cgaaaaccgg     1080

gccaaagucg aaauaacacc gaauagcccc cgcgccgaag caacccucgg cggauucgga     1140

ucccucgggu uagacugcga gccccgcacu ggccucgacu ucuccgaccu auacuaccuc     1200

accaugaaca acaaacacug gcugguccac aaagaguggu uccacgacau cccccugcca     1260

uggcacgcag gagccgacac cggcacaccc cacuggaaca auaaagaagc ucuaguagag     1320

uucaaggacg cacaugcaaa acgacaaacc gucgucgucc uaggcuccca agaaggagca     1380

guacacaccg cccucgcagg agcccucgaa gccgaaaugg acggagccaa agggcgacuc     1440

uccucaggac accuaaaaug ucgccuaaaa auggacaagc uaaggcucaa aggaguaagc     1500

uacucccucu gcaccgcggc auucaccuuc acaaaaaucc ccgcugaaac acuccacggc     1560

accgucacag uggaaguaca auacgccgga acagacgggc cgugcaaagu accagcccaa     1620

auggcggucg acaugcagac ccucacccca gucgggcgcc ucaucacggc caacccagua     1680

auuacggaau caaccgagaa cuccaaaaug augcucgaac uggauccccc auucggcgac     1740

agcuacaucg ucaucggcgu uggcgaaaag aaaauaacac accacuggca cagauccggc     1800

agcacacucg gcaaagcguu cucuacaaca cucaaaggug cacaacgacu agcugcacuc     1860

ggcgacacag caugggacuu cggauccauc ggcggagucu ucaacuccau cgggagagca     1920

guccaccagg uuuucggggg cgccuuccga acacuauucg gagggaugag cuggaucacg     1980

caaggacuaa ugggagcccu ccuacuaugg augggcguaa augcaagaga ccggagcaua     2040

gcacucgccu uccuagcaac cggaggcguc cucgucuucc uagccacaaa cguccacgcg     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10294
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10294
gggagaaagc uuaccauggc caucagcggc guccccgucc uaggcuuuuu caucauugcc       60

guacucaugu cagcccaaga aagcugggcc guaacccgac gcggcagcgc cuacuacaug      120

uaccuagacc gaaacgaugc aggggaagca aucuccuucc ccaccacgcu aggaaugaac      180

aaaugcuaca uucaaauaau ggaccucggc cauaccugcg acgccaccau gucauacgag      240

ugccccaugu uagacgaagg aguagaaccc gacgaugugg auugcuggug caacacaaca      300

agcaccuggg ucguguacgg gaccugccac cacaagaaag gagaagcgcg cagaucacgc      360

agggcaguca cgcuacccag ccacucaaca cggaaacugc aaacccgcuc ccaaacaugg      420

cuagagucac gggaauacac caaacaccug auccgagucg aaaacuggau cuuuagaaac      480

ccaggauucg cccucgccgc agcugcuauc gccuggcucc ucgggucauc aacaucccag      540

aaaguuaucu aucucguaau gauccuccua aucgcccccg ccuacuccau ccgcugcaua      600

ggagugagua acagagacuu cguggaaggc auguccggcg ggacaugggu agacgucgua      660

cucgaacacg ggggaugcgu aacagugaug gcucaagaua aaccaacagu agacaucgaa      720

cucguuacga caaccgucag caacauggca gaaguccgaa gcuacugcua cgaggcaagc      780

auaagcgaca uggccucaga cagccgaugc ccuacccaag gcgaagcaua ccuagacaaa      840

caaagcgaca cccaauacgu cugcaaaaga acgcucgugg accguggcug gggaaacggc      900

ugcggccuau ucggaaaagg aagucuggua accugcgcca aauucgcgug cuccaagaaa      960

augaccggca aauccaucca accugaaaac cucgaauacc ggaucaugcu auccguccac     1020

ggcagccaac acucaggcau gaucgucaac gacacaggac acgaaaccga cgaaaaucga     1080

gccaaaguag aaauaacacc caacagccca agagcagaag caacacuagg cggauucggg     1140

agccucggac ucgacugcga gccacgcaca gggcuagacu ucucagaccu cuacuaccua     1200

accaugaaca acaaacacug gcucguccac aaagaauggu uccacgacau accacucccc     1260

uggcacgcag gggccgacac cggcacgcca cacuggaaca acaaagaggc acuuguagag     1320

uucaaagacg cccacgcaaa gcgacaaacc gucguaguuc ucggauccca agaaggcgcc     1380

guccacacgg cccucgccgg agcacuagaa gcagaaaugg acggcgccaa aggaaggcua     1440

aguuccggcc accucaaaug uagacucaaa auggacaaac uucgacuaaa aggagucagc     1500

uacagcuuau gcaccgccgc auucacauuc acaaaaaucc cagcagaaac acuacacgga     1560

accgucacag ucgaaguaca auacgcgggc acagacggcc cauguaaggu gcccgcccaa     1620

auggcagugg acaugcaaac ccuaaccccc guagggcgac ucaucacggc aaacccaguc     1680

aucaccgaaa gcacagaaaa cagcaaaaug augcucgaac ucgacccacc uuucggggac     1740

uccuacauag ucaucggcgu cggcgaaaag aaaauaaccc accacuggca cagaucaggc     1800

aguacccuug gaaaagccuu cucaaccacu uuaaaaggug cacaaagacu agcugcacuc     1860

ggcgacaccg caugggacuu cggcucgaua ggcgggguau ucaacagcau aggacgagcg     1920

guccaccaag ucuucggcgg cgcauuccgg acacuguucg gaggaauguc cuggauaacc     1980

cagggacuca ugggggcccu acuccucugg augggaguaa acgcgagaga ccgcuccauc     2040

gcccuagcau uccuagccac aggaggcguc cuaguuuucc ucgcaacaaa cguacaugcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10295
<211>  2293
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10295
gggagaaagc uuaccauggc caucucaggg guaccaguac uaggcuucuu caucauagcc       60

guccucaugu cagcccaaga aucaugggcg auaacacgcc gaggcagcgc uuacuauaug      120

uaucucgauc gaagcgacgc cggaaaagcc aucaguuucg cuaccacucu aggaguuaac      180

aaaugccacg uacaaaucau ggaucugggc cacaugugcg acgccaccau gagcuacgag      240

ugcccaaugc ucgacgaagg aguagaaccg gacgacgucg acugcuggug caacaccacc      300

aguacauggg uugucuacgg cacuugccac cacaagaaag gagaagcccg acgaucccgc      360

cgcgcgguca cucuccccuc ccacuccaca cgaaaacucc aaacccgcuc acaaacgugg      420

cuagagucgc gagaauacac caaacaccuc auaaaaguag aaaacuggau cuuccgcaac      480

ccaggcuucg cacucguagc gguugcgauc gcguggcucc uaggcuccuc cacuucccaa      540

aaagucaucu accuaguuau gauccuccua aucgccccag ccuacagcau ccggugcauc      600

ggagucucaa acagagacuu cgucgaaggu augagcggcg gcacaugggu agacgugguc      660

cucgaacacg gcggaugcgu cacaguuaug gcgcaagaca aacccaccgu agacaucgaa      720

cucgucacga ccaccguguc aaacauggcc gaaguccgaa gcuacugcua cgaagcauca      780

auaucagaca uggccuccga cucccguugc ccaacgcaag gagaagccua ccucgacaaa      840

caaagcgaca cccaauacgu uugcaaacgc acccuagucg accgcggcug gggcaacgga      900

ugcggccucu ucggaaaagg cucccucgug acaugcgcaa aauucacaug cagcaagaaa      960

augacaggaa aaucaaucca acccgaaaac cucgaauaca gaaucauguu gucaguacac     1020

ggcagccaac acucaggaau gauaggauac gaaacggacg aagaccgcgc aaaagucgaa     1080

gucaccccaa acagccccag ggccgaggcg acccuuggag gauucggcag ccucggacuc     1140

gacugcgagc cccgcacagg ccucgauuuc agcgaucuau acuaccuuac caugaauaac     1200

aaacacuggu uaguccacaa agaaugguuc cacgacauac cccuccccug gcacgcggga     1260

gcggacaccg guaccccaca cuggaacaac aaagaagcac ucgucgaguu caaagacgcc     1320

cacgccaaaa ggcaaacagu aguaguccuc ggaagucaag aaggcgccgu ccacaccgca     1380

cuggccggcg cccucgaagc cgaaauggau ggagccaaag gccgacucuu cucaggacac     1440

cucaaaugcc gacucaaaau ggacaaacuc agacuaaaag gaguauccua cagccuuugc     1500

acagccgccu ucacguucac aaaagucccc gccgaaacac uucacggaac ggucacaguc     1560

gaaguacaau acgccggcac cgacggcccc ugcaaaaucc ccguucaaau ggcgguggac     1620

augcaaacuc ucacaccugu aggacgacua auuaccgcca accccgucau cacugaaucc     1680

acagaaaaca gcaaaaugau gcuagaacuc gaucccccau ucggcgacag cuacauagua     1740

aucggugucg gcgacaagaa aaucacacac cacuggcacc gcagcggcuc cacacuugga     1800

aaagcauuuu caaccacccu caaaggcgca caaagacuag ccgcgcucgg agacacagca     1860

ugggacuucg gcucgaucgg cggagucuuc aacuccauag gacgagccgu ccaccaggua     1920

uucggcggag ccuuccgcac acuguucgga ggcaugagcu ggaucacuca aggacucaug     1980

ggagcgcucc uacucuggau gggcgucaac gcccgcgacc gaagcauagc uuuagccuuc     2040

cucgccacag gaggcguccu cgucuuccuc gcgaccaacg uacacgccug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     2280

agccaccaga auu                                                        2293


<210>  10296
<211>  2305
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10296
gggagaaagc uuaccauggc gaucuccggc guaccagucc uaggauucuu caucaucgca       60

guccucaugu cugcacaaga aucaugggca guaacaagac gcggguccgc auacuacaug      120

uaccucgauc gaaacgaugc cggcgaagcc auauccuucc ccacaacacu aggcaugaac      180

aaaugcuaca uccaaaucau ggaccuagga cacaugugcg acgcaacaau guccuacgaa      240

ugcccaaugc uggacgaggg gguggaacca gacgacguag auuguuggug caacacaacc      300

uccaccuggg ugguauacgg aacgugccac cacaaaaagg gagaagcccg acgcagccga      360

cgagccguga cccuacccuc ccacagcaca cgaaaacuac aaacacgcag ccaaacaugg      420

cuagaaagca gagaauacac caaacaccua auacgcguag aaaacuggau cuuccgcaac      480

ccaggcuucg cacuggccgc agccgccauc gcauggcucc ucggcagcuc aaccucccaa      540

aagguaaucu accuuguuau gauccuccua aucgcaccgg cguacuccau aagaugcauc      600

ggcgucucaa auagggacuu cguagaaggc augagcgggg gaacgugggu agacguaguc      660

cucgaacacg gaggcugcgu aaccgugaug gcacaagaca aaccaacggu agacaucgag      720

cuaguaacaa caaccgucuc caacauggcu gaagucagau ccuacuguua cgaagccagc      780

auaagugaca uggccucaga cucacgcugc cccacacaag gcgaagcaua ccuagacaaa      840

caauccgaca cccaguacgu cugcaagcga acccucgucg acagaggcug gggcaacgga      900

uguggccuau uuggaaaggg aagccucgua accugcgcua aauucgccug cucaaagaaa      960

augacaggca aaagcaucca acccgaaaac cuggaauacc gcaucaugcu aagcguccac     1020

ggcagccaac acucagggau gauaguaaau gauaccggac acgaaacaga cgaaaaccga     1080

gcaaaagugg aaauaacacc aaacucgcca cgggccgaag ccacgcuagg gggcuucggc     1140

ucacuagggc ucgauugcga gccaagaacc ggacuagauu ucagcgaccu auacuaucua     1200

acgaugaaca acaaacauug gcuaguacac aaagaauggu uccacgacau cccccuacca     1260

uggcacgccg gcgccgacac cggcacacca cacuggaaca acaaagaagc acucguggag     1320

uucaaagaug cccacgccaa aagacaaaca gucgugguac uaggcaguca agaaggcgcc     1380

gugcacacag cucuugcggg agcccuagaa gccgaaaugg acggcgccaa aggaagacua     1440

agcuccgggc accucaaaug ccgacuaaaa auggacaaac ucagacugaa aggcgucagc     1500

uacucacucu gcacagcagc cuucacauuc accaaaauuc ccgcagaaac acuacacggc     1560

acaguaaccg ucgaagucca auacgcaggc acagacggcc ccugcaaagu ccccgcacaa     1620

auggccguag acaugcaaac acuaacaccc guaggucgac uaauaaccgc gaacccaguc     1680

aucacagaau ccacugaaaa cagcaaaaug augcuagaac ucgacccgcc auucggagac     1740

ucauacaucg uaaucggagu cggagagaag aaaauaacac accauuggca ccgcucgggc     1800

uccacacuag gcaaagccuu cagcacaacc cucaaaggag cacaaagacu ggccgcacuc     1860

ggcgacaccg cgugggacuu cggcagcauc ggaggcgugu ucaacucaau aggacgugcc     1920

guacaccaag ucuucggcgg agccuuccga acacuauucg gcgggauguc auggauaacc     1980

caaggccuaa ugggcgcccu acuccuaugg augggaguaa acgcacggga ccgcucgaua     2040

gcacuagcuu uccucgcaac gggcggcguc cuaguguuuc uagcaacaaa cguucacgcc     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2280

ggcucuuuuc agagccacca gaauu                                           2305


<210>  10297
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10297
gggagaaagc uuaccaugga cuggaccugg auacuauucc ucguagccgc cgccacccgg       60

guccauucag ucacccgccg aggcucugcc uacuacaugu aucuggacag aaacgacgcc      120

ggcgaagcaa uuagcuuccc aacaacccuc ggaaugaaca aaugcuacau acaaauaaug      180

gaccucggcc acaugugcga cgcaaccaug ucauacgagu gcccaaugcu ggacgaggga      240

gucgaacccg acgacguaga cugcuggugc aacaccacca gcaccugggu cguauacggc      300

acaugucacc acaagaaagg ggaagcccga cguagccgca gagcaguaac ccuaccaagu      360

cauucaacac gcaaacucca aacaaggagc caaaccuggc uagaaucacg ggaguacaca      420

aaacaccuca uacgaguaga aaacuggauc uucagaaacc cgggguucgc ccucgcagcc      480

gccgccauag cauggcuucu cgggucaagc accucccaaa aaguaaucua ccucgucaug      540

auacuacuaa ucgcaccagc auacagcaua cgaugcaucg gcguaucaaa cagagacuuc      600

guagaaggca ugaguggagg gaccuggguu gacauaguac uggaacacgg cggaugcguc      660

acagucaugg cacaggacaa accaaccguc gacauagagc uuguuacaac cacggucagc      720

aacauggccg aaguccgaag cuacugcuac gaggcaagca uaucagacau ggccucagac      780

agccgaugcc caacgcaggg agaagcauac cucgacaaac aaagcgacac gcaauacguc      840

ugcaaaagga cgcuuguaga ccgcggcugg ggaaacggau gcggccucuu uggaaaagga      900

ucucuaguaa ccugcgcaaa guucgcgugc ucaaagaaaa ugacuggaaa aucaauccaa      960

cccgagaacc ucgaauaccg caucaugcua uccguccacg ggucacaaca cagcggaaug     1020

aucguaaacg acacaggaca cgaaacagac gaaaacaggg ccaaaguaga gaucaccccg     1080

aacagcccaa gagccgaagc aacacuaggc ggcuucggca gucuuggacu cgacugcgaa     1140

ccgcggacug gacuggacuu uagcgaccug uacuaucuca ccaugaacaa caaacacugg     1200

cuaguacaca aagaaugguu ccacgacauc ccgcucccau ggcacgcagg cgccgauacc     1260

ggaaccccuc acuggaacaa caaagaagcc cucguagagu ucaaagacgc ccacgccaaa     1320

cgacaaacag uagucgugcu cggaagccaa gaaggagcgg uacacacggc acucgcggga     1380

gcccuggaag cggaaaugga cggcgcaaaa ggacgacuau caagcgguca ccucaaaugc     1440

cgacuaaaaa uggacaagcu acgccucaaa ggcgucagcu auagccuaug cacagccgca     1500

uucacauuca caaaaauacc ggccgaaaca cuacacggca ccgucaccgu cgagguacaa     1560

uacgcaggua ccgacggacc cugcaaaguc ccagcccaga uggcagucga caugcaaacc     1620

cucaccccug ucgggaggcu aaucaccgca aaccccguaa uuacagaauc aacagagaac     1680

agcaaaauga ugcuggaacu ggacccaccc uucggcgacu ccuacaucgu caucggagua     1740

ggugaaaaga aaaucaccca ccacuggcac cgaagcggcu caacccuagg caaagccuuc     1800

agcacaaccc uaaaaggagc ccaacgccuc gccgcccucg gcgauacggc gugggacuuc     1860

ggcuccauag gaggcguauu caauucaauc ggccgcgccg uacaccaagu cuucggcgga     1920

gcauuccgga cacucuucgg aggaaugagc uggauaacgc aaggacucau gggcgcauua     1980

cucuuaugga uggggguaaa cgcccgagac cguagcaucg ccuuagccuu ccucgccaca     2040

ggcggagugc uaguauuccu cgcaacaaac guccacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10298
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10298
gggagaaagc uuaccaugga cuggaccugg auacucuucc ugguagccgc agcuacccgc       60

guacacucag ucacacgccg aggaucggcc uacuacaugu acuuagaccg caacgacgcu      120

ggcgaagcaa uaucauuccc cacaaccuua ggcaugaaca aaugcuacau acaaaucaug      180

gaccugggcc acacauguga cgcgaccaug uccuacgagu gcccaaugcu ggacgaagga      240

guagagccgg acgacguaga cugcuggugc aacaccacca gcacaugggu agucuacgga      300

accugccacc acaagaaagg cgaggcccgc cgaagccgaa gggcagucac ccucccaagc      360

cacuccaccc gaaaacuaca gacccgcucc caaaccuggc uagaaucucg ugaauacaca      420

aaacaccuca ucaggguaga aaacuggauc uuccgcaacc ccggcuucgc acucgcagca      480

gccgcaaucg cuuggcuacu gggcuccucc accucacaaa aaguaauaua ccuggucaug      540

auccuacuga ucgccccagc cuacagcauc cgcugcaucg gcguaagcaa ccgggacuuc      600

guagaaggca ugucuggcgg gaccugggua gacgucgugc uagagcacgg cggcugcguc      660

acaguaaugg cacaagacaa gcccacaguc gacaucgaac ucgucaccac caccguaagc      720

aacauggcag agguccgcuc cuauugcuac gaggccagca uaucagacau ggcaagcgac      780

agccggugcc caacccaagg cgaagcauac cucgauaaac aauccgacac gcaauacgua      840

ugcaaacgaa cacucgucga cagaggcugg ggaaacggau gcggccuauu cggaaaagga      900

ucccuaguca ccugugccaa auucgccugc aguaagaaaa ugacaggcaa aagcauacaa      960

ccagaaaacc uagaauaccg aaucaugcuc uccguacacg gaagccaaca cucaggaaug     1020

aucgucaacg acacugggca cgaaaccgau gaaaaccgug caaaaguaga aauaacgccc     1080

aacucaccac gcgcagaagc cacccuggga ggcuucggca gccuaggacu cgacugcgaa     1140

ccaagaacug gccucgacuu cucagaccuc uacuaccuca ccaugaacaa caaacacugg     1200

cucguacaca aagagugguu ccacgacauc ccccuaccau ggcacgcugg ggcggacaca     1260

ggcacacccc acuggaacaa uaaagaagcc cuaguagagu ucaaagacgc acacgcaaaa     1320

cgacaaaccg uagucguacu uggcagccag gaaggugcgg uccauaccgc acuagccggc     1380

gcccucgaag ccgaaaugga cggcgcuaaa ggucgccucu caagcggcca ucuaaagugc     1440

cgacuaaaaa uggacaaacu ccgucucaaa ggagucuccu acagccugug caccgcagca     1500

uucaccuuca caaaaauccc agcggaaacc cuccauggaa ccgugaccgu cgaaguccaa     1560

uacgcaggaa cagauggacc cugcaagguc cccgcccaaa uggcaguaga caugcaaaca     1620

cugacccccg ucggccgacu cauaacggcc aacccaguca uaacugaauc cacagaaaac     1680

ucaaaaauga ugcuggagcu cgacccgccc uucggcgaua gcuacauagu caucggcgug     1740

ggcgaaaaga aaauaacuca ccacuggcac cgcagcggca guacacuagg caaagccuuc     1800

aguaccaccc ugaaaggagc ccaacgacuc gcagcccucg gagacacagc augggacuuc     1860

gguagcaucg gcggcgucuu caacuccauc gggagagccg ugcaccaagu guucggaggc     1920

gccuuccgua cccucuucgg aggaauguca uggauaacuc aagggcucau gggcgcccuc     1980

cuacucugga ugggaguaaa cgccagagac agaagcaucg cccuagccuu ccuagccacc     2040

ggcggaguac ucguauuccu ggcaaccaac guccacgcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10299
<211>  2272
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10299
gggagaaagc uuaccaugga uuggaccugg auccuguucc ucguagccgc cgccacacgc       60

guacauucaa uaacacgccg aggaucggca uacuacaugu acuuggaccg uagcgacgca      120

ggcaaagcga ucuccuucgc cacaacccug ggcgucaaua aaugccacgu acaaaucaug      180

gaccuuggac acaugugcga cgccacaaug ucauacgaau gccccaugcu ggacgagggc      240

guagaaccag acgacgucga cugcuggugc aauaccacaa gcacaugggu cguuuacggc      300

acaugccacc acaagaaagg ggaagcgcga cggucccgac gagcggugac acuaccaucc      360

cacagcacac gcaaacuaca aacaaggagc caaaccuggc ucgaaagccg agaguacaca      420

aaacaccuaa uuaaaguaga aaacuggauc uuccgcaauc caggauucgc gcuaguagca      480

gucgccauag ccuggcuacu aggaagcagc accucccaga aagucaucua ucuagucaug      540

auccucuuaa uagcacccgc guacaguaua cgaugcauag gcguaagcaa cagggauuuc      600

guagaaggaa ugucgggagg gaccugggua gacgucgugc ucgagcacgg cgggugcgua      660

accguaaugg cccaagacaa acccacaguc gacaucgaac ucguuacaac cacaguuagc      720

aauauggccg aaguaagguc auacugcuac gaagcaucga ucagugacau ggcaucagac      780

ucacggugcc ccacgcaagg cgaagcauac cuagacaaac aaagcgacac ccaauacguc      840

ugcaaacgaa cacucgucga ccgaggcugg ggaaacgggu gcggacucuu cggcaaaggc      900

ucgcugguaa ccugcgcaaa auuuacaugu ucaaagaaaa ugacaggcaa aagcauucaa      960

cccgaaaacc ucgaguaccg aaucaugcua ucaguccacg gaagccaaca cagcggaaug     1020

aucggguaug aaaccgacga ggacagagcc aaaguagaag uaacgccaaa cagcccccgg     1080

gccgaagcca cacugggcgg auucggaagc cucggccugg acugcgaacc cagaacaggc     1140

cuggacuuca gcgaccuaua cuaccuaacc augaacaaca aacacuggcu cguacacaaa     1200

gagugguucc acgacauccc ccucccaugg cacgcaggag ccgacaccgg cacaccccac     1260

uggaacaaca aagaagcccu ggucgaguuc aaagacgccc acgcgaaacg acagaccgua     1320

gucgugcuag gaagucaaga gggggcagua cacacagccc ucgccggagc acucgaggcc     1380

gagauggacg gcgcaaaagg acgccucuuc ucgggacacc uaaaaugccg ccucaaaaug     1440

gauaaauuaa gacuaaaagg cgucucauac ucguuaugca ccgccgcauu caccuucacc     1500

aaaguccccg ccgaaacccu acauggaaca guaaccgugg aaguacagua cgcggggacc     1560

gacggacccu gcaaaauccc aguccagaug gcggucgaca ugcaaacccu gacgcccguc     1620

ggacgccuaa uaacggcuaa cccggugaua accgaaucca cagaaaacuc aaaaaugaug     1680

cucgaacuag acccaccauu cggagacagc uacaucguaa uaggagucgg cgacaagaaa     1740

aucacccacc acuggcaccg aaguggaucc acacuaggca aagcauucuc aaccacacuc     1800

aaaggggccc agcggcucgc cgcccuagga gacacagcau gggacuucgg aucaaucgga     1860

gguguauuca acucgauagg cagagccguc caccaaguau ucggaggggc auuccgaaca     1920

cuauucgggg gaaugagcug gaucacacaa ggacucaugg gagcccugcu gcuuuggaug     1980

ggcguaaacg ccagagaccg aucaauagcc cuagcauucc uugcaacagg aggcguccuc     2040

gucuuccuag caacaaacgu acacgcauga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     2220

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2272


<210>  10300
<211>  2284
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10300
gggagaaagc uuaccaugga cuggacaugg auccucuuuc ucguagcggc cgcaacccga       60

guccacagcg ugacccgccg cggcuccgcc uacuacaugu accucgacag aaacgacgcc      120

ggcgaggcaa ucuccuuccc gaccacccuc ggaaugaaua aauguuacau acaaaucaug      180

gaccucgggc acaugugcga cgcaaccaug agcuacgaau gccccaugcu ggacgaagga      240

guagaaccag acgacgucga uugcuggugc aacaccacaa gcaccugggu aguauauggc      300

acaugucacc acaaaaaggg ggaagcccgc cgcucacgcc gcgccguaac acucccauca      360

cacagcaccc gcaaacucca aaccagaucc caaaccuggc ucgaaucaag agaguacaca      420

aagcaccuaa uacgcguaga aaacuggauu uucagaaacc caggcuucgc ucuugcagcc      480

gccgcgaucg ccuggcuccu cggcuccagu acaagccaga aggucaucua ccucguaaug      540

auccuccuaa ucgcaccagc cuacuccaua cgcugcauag guguaucaaa ccgagacuuc      600

guagaaggaa ugagcggcgg aacauggguc gacguaguac uagaacacgg cggcugcguc      660

acagucaugg cacaggauaa accaacgguc gacaucgaac ucguaaccac aacaguaagc      720

aacauggcag agguccgaag cuacugcuac gaggccucca uauccgacau ggcauccgac      780

uccaggugcc ccacccaagg agaagccuau uuagacaaac aauccgacac ccaauacguc      840

ugcaaaagaa cacuggugga ucgaggaugg gggaacggcu gcggauuauu cggaaaagga      900

ucacucguaa ccugcgcaaa auucgcaugc agcaagaaaa ugacaggcaa aagcauccag      960

ccggaaaacc ucgaauaccg caucaugcua ucaguacacg gaucccaaca cuccggcaug     1020

aucgugaacg acacaggaca cgaaacggac gaaaaccggg ccaaggucga aauaacaccu     1080

aacucacccc gagccgaagc gacucucgga ggguucggga gccucggccu cgacugcgag     1140

ccacgcaccg gccuagacuu uucagaccuc uauuaccuca ccaugaacaa caaacacugg     1200

cuaguccaca aagaaugguu ccacgacauc ccacuaccuu ggcacgccgg cgccgacaca     1260

ggcacacccc acuggaauaa caaagaagca cuggucgaau uuaaagacgc acacgccaaa     1320

cgacaaacag ucgucguacu aggaucacaa gaaggagcag uucacaccgc ccuagccgga     1380

gcacuugaag cagaaaugga cggagcaaaa ggacgccuua gcucaggaca ccuaaagugc     1440

aggcugaaga uggacaaacu gagacucaaa ggagucuccu acucacugug caccgcagcc     1500

uucacauuca caaagauccc cgcagagacu cuccacggaa ccgucaccgu cgaaguacaa     1560

uacgcaggaa cagacggccc uugcaagguc cccgcccaaa uggcagucga caugcagaca     1620

cucaccccag ucggaagacu caucacagcc aaccccguca uaaccgaauc caccgaaaac     1680

ucaaaaauga ugcucgaacu ggacccaccc uucggugaca gcuacaucgu cauaggggua     1740

ggagaaaaga aaaucacaca ccacuggcac cgcucaggau ccacccucgg aaaagccuuc     1800

ucaaccacac ucaaaggcgc acaaagacuc gcggcacucg gagacaccgc gugggacuuc     1860

ggauccaucg gcggcguauu caacagcaua ggacgcgcag uccaccaagu auucggagga     1920

gccuuucgaa cacucuucgg cggaauguca uggaucaccc aaggccuaau gggcgcccua     1980

cuccucugga ugggagugaa cgcccgcgac cggagcaucg cacuagcuuu cuuggcaaca     2040

ggcggcgucc uaguauuccu ggccaccaac guacacgccu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2280

aauu                                                                  2284


<210>  10301
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 1 opt

<400>  10301
gggagaaagc uuaccauggg caaaagguca gcgggcagca ucauguggcu cgccucccuc       60

gcuguaguga ucgccugcgc aggagcggua acgcgccgag gcucagcaua cuacauguau      120

cuggaccgaa acgaugccgg agaagcaaua uccuucccaa caacacuagg caugaacaaa      180

ugcuacauac aaauaaugga ccuaggccac augugcgacg caacgauguc auacgaaugc      240

ccaaugcucg acgaaggcgu ugaaccagac gacgucgacu gcuggugcaa cacaacgagc      300

acgugggucg uauacggcac augccaccac aagaaaggag aagcacgccg gagccgucgg      360

gcagucaccc ugccauccca cuccacaaga aaacuccaaa cacgaagcca gacauggcuc      420

gagagcagag aauacaccaa acaccuaauc agagucgaaa acuggauauu ccgaaacccc      480

ggguucgcgc ucgcagccgc cgcaaucgcc uggcuacuag gaucaucaac cagccaaaaa      540

guaaucuauc uaguaaugau acuacuaaua gccccggcgu acuccauaag augcauaggc      600

gucaguaacc gagauuucgu agaaggaaug ucaggaggga caugggucga caucguacua      660

gagcacggcg gcugcgugac cguaauggca caggacaaac caacgguaga caucgaacuc      720

gucaccacaa ccguaaguaa cauggcagaa gugagaucau auugcuacga agccuccaua      780

agcgacaugg ccagcgacuc uagaugucca acgcaaggag aagcguaccu cgacaaacag      840

uccgacacgc aauacgucug caaacgcaca cucguggacc gcggcugggg aaacggaugc      900

ggacuauucg gaaaaggguc ccuagucaca ugcgcaaaau uugcgugcuc aaagaaaaug      960

acaggcaaau ccauccagcc agaaaaccua gaauacagaa ucaugcucuc cguacacggc     1020

ucccaacaca gcggaaugau cguaaacgac acaggacaug aaacggacga gaacagagca     1080

aaagucgaaa ucacgcccaa uagcccacgc gccgaagcaa cccuuggagg cuucggcagc     1140

uugggccuug acugcgaacc acgcacgggg cuagacuucu cagaccuaua cuaccuaaca     1200

augaacaaca aacacuggcu cguccacaaa gagugguucc acgacauccc ccucccgugg     1260

cacgccggcg cagauaccgg cacaccccac uggaacaaca aagaagcccu agucgaguuc     1320

aaagacgcac acgccaaaag acaaacaguc gugguacuag gaucccaaga aggagcaguc     1380

cacacagcgc uagcaggcgc ccuagaggcc gagauggaug gagcgaaagg acgucuaucc     1440

ucaggacacc uaaaaugcag gcuaaaaaug gacaaacuac gacuaaaagg cgucucuuac     1500

ucacuaugca cagccgcauu cacauuuacc aaaaucccag cggaaacacu gcacggcaca     1560

guaaccguag aaguacaaua cgcaggcacc gacggacccu guaagguccc agcccaaaug     1620

gcagucgaca ugcaaacacu gaccccgguc ggacgccuga uaacagccaa cccaguaauc     1680

acagaaucga cagaaaauuc caaaaugaug cuagaacuag aucccccauu cggcgacagc     1740

uauaucguga ucggaguagg ggaaaagaaa aucacccacc acuggcaccg auccgggagc     1800

acgcucggaa aagcguucuc aaccacacug aaaggggcac agcggcucgc agcacucggc     1860

gacaccgcau gggacuucgg gucaaucgga ggcgucuuca acuccaucgg acgagcagua     1920

caccaagucu ucggaggcgc guucagaaca uuauuugggg gcaugagcug gaucacacag     1980

ggccucaugg gcgcccuacu ccucuggaug ggggugaacg ccagagacag auccauugca     2040

cuagcguucc uagcaacggg cggcguacuc guauuccuag ccacaaacgu ccaugccuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10302
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 1 opt

<400>  10302
gggagaaagc uuaccauggg gaaaagauca gcaggcucca ucauguggcu cgccucacuc       60

gcaguaguca uagcaugcgc aggcgcgguc accagacgag gcucagcgua cuacauguac      120

cuugacagga acgacgcagg agaagccauc agcuucccga ccacacucgg caugaacaaa      180

uguuacaucc aaaucaugga ccuaggacau accugcgacg ccacaauguc auacgaaugc      240

ccaaugcuag acgaaggcgu agaacccgac gacgucgacu gcuggugcaa caccacgagc      300

accugggucg ucuacggcac augccaccac aagaaaggag aagcccgccg aucccgccga      360

gcagucaccc uccccagcca cucuaccaga aaacuacaaa ccagaagcca gaccuggcuc      420

gaaucaaggg aauacacaaa acacuuaauc agggucgaaa acuggauauu ccguaaccca      480

ggguucgccc ucgcggcagc cgccauagca uggcuacugg gcucgagcac uagccagaaa      540

guaaucuacc ucgucaugau auuacugauc gcaccagccu acucaaucag augcauaggc      600

guaagcaacc gcgacuucgu agaagggaug ucagguggaa caugggucga cgucguacuc      660

gaacacggcg guugcgucac aguaauggcu caggacaagc ccacgguaga cauagaacuu      720

guaacaacaa ccguaagcaa cauggccgaa guacgcuccu acugcuacga agccucaauc      780

ucagacaugg cauccgacuc caggugucca acgcaagggg aagcguaccu agacaaacaa      840

uccgacacac aauacgucug caaaagaacc cuggucgacc gaggcugggg caacggaugc      900

gggcuauuug gcaaaggcuc ccuaguaacc ugugccaagu ucgcaugcuc caagaaaaug      960

accggcaaau ccauacaacc cgaaaacuua gaauacagaa uaaugcuauc uguucacgga     1020

ucccaacaca gcgggaugau agucaaugac acaggacacg aaacagacga gaacagggcc     1080

aaaguagaaa uaacccccaa cagcccacgc gcagaagcua cacugggcgg auucggaucu     1140

cuaggccuag acugcgaacc acgcaccggc cuagacuucu cagaccucua cuacuugacc     1200

augaauaaca aacacugguu aguccacaaa gaaugguucc acgacauacc acuccccugg     1260

cacgccggcg cagacacagg caccccacac uggaacaaca aagaagcccu cgucgaguuc     1320

aaagacgcac acgcgaaacg gcagaccguc guaguccucg gaagccaaga aggagcgguc     1380

cacacagccc uagcaggcgc acuagaagca gaaauggacg gcgcgaaagg gcgacugucc     1440

ucagggcacc ucaaaugucg gcucaaaaug gacaaacuac gccuuaaagg cguaucguac     1500

ucacuaugca ccgcggccuu cacauucacc aaaauccccg ccgaaacacu acauggaacc     1560

guaaccgucg aaguacaaua cgcaggcacc gaugggcccu gcaaaguccc ugcacaaaug     1620

gcagucgaca ugcaaacacu caccccaguc ggccgacuaa ucacagcuaa ccccgugaua     1680

acggagucca cagaaaacuc gaaaaugaug cucgaacuag acccgcccuu cggcgacagc     1740

uacauaguua ucggagucgg ugaaaagaaa auaacccacc acuggcaccg aagcggaagc     1800

acccucggaa aagccuucuc caccacccuc aaaggcgccc aaagacuagc agccuuaggc     1860

gacaccgcgu gggacuucgg aucuauaggc ggaguauuca acuccauagg acgcgcaguc     1920

caccaaguau ucgggggagc auucagaaca cucuucggcg guaugagcug gaucacacaa     1980

ggccucaugg gagcacuccu acucuggaug ggcgucaacg caagggacag auccaucgcc     2040

cucgcguuuc uagcgaccgg gggagugcuc guauuccucg ccacgaacgu ccacgcauga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10303
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 1 opt

<400>  10303
gggagaaagc uuaccauggg aaaacgcagc gccggaucca ucauguggcu agcaucgcuc       60

gcaguaguaa ucgcgugcgc cggugcaauc acacgacgcg gcagcgcaua cuauauguac      120

cucgaccgaa gugaugccgg gaaggcgauc ucauuugcaa ccacgcuagg cguaaauaaa      180

ugccacgucc agaucaugga ccucggccac augugcgacg ccaccaugag cuacgaaugc      240

ccgaugcugg acgaaggcgu ggaacccgau gacgucgacu gcuggugcaa cacaacaagc      300

accuggguug ucuacggcac gugccaccac aagaaaggcg aagcaagaag gucacgacga      360

gcaguaacgc uaccaagcca cagcacacgc aaacuacaaa ccagaucaca aacauggcuc      420

gagagccgcg aauacaccaa acaccuuaua aaaguagaaa acuggauauu cagaaacccg      480

ggauucgccc uaguagcagu agcaauagca uggcuacugg gcucguccac gagucaaaaa      540

guaauauacc uaguaaugau acuacugaua gcacccgccu acucaauccg gugcaucgga      600

guauccaaca gggacuucgu agaagggaug ucaggcggga cauggguaga cgucguccua      660

gagcacggcg gaugcgucac gguaauggcc caagacaaac ccacaguaga uaucgagcua      720

guaaccacga ccguaagcaa cauggccgaa gucagaucgu acugcuacga agccagcauc      780

agcgacaugg ccuccgacag ccgcugccca acccaagggg aagccuaccu agacaaacaa      840

uccgacacac aauacguaug caaaaggacc cuaguagaua gagggugggg aaacggaugu      900

ggucucuucg gcaaaggcuc ccucguaaca ugcgccaaau ucacaugcag caaaaagaug      960

acaggcaagu caauacagcc agaaaaccuc gaguacagaa ucaugcucuc aguccacggu     1020

ucacaacaca gcggaaugau cggcuacgaa acggaugaag acagagcaaa aguggaagua     1080

acaccuaaca gcccacgcgc agaagcgaca cuaggaggau ucgggagucu aggauuggac     1140

ugcgaaccaa gaacaggccu agacuucagc gaccucuacu accucacaau gaacaacaaa     1200

cacuggcuag uccacaagga augguuccac gacauaccac uaccauggca cgcgggcgcc     1260

gacaccggaa caccgcacug gaacaacaaa gaagcccucg ucgaguucaa agacgcgcac     1320

gcaaaacgac aaacgguagu aguccugggc ucacaggaag gcgcaguaca cacagcgcua     1380

gccggagccc uagaggcaga aauggacggc gcaaaaggcc gccuguucag cggacaccua     1440

aaaugcagac ucaaaaugga caaacuacgc cuaaaaggcg ugucauacag ccucugcacg     1500

gccgccuuua cguucacaaa agugccagca gaaacacugc acggcacagu uacaguagaa     1560

guacaguaug ccggaaccga cggcccaugc aaaaucccug ugcagauggc cguggacaug     1620

caaacacuga caccgguagg acgacuaaua accgcaaauc cagugauaac ggaaucaaca     1680

gaaaacucca aaaugaugcu ggaacucgac ccaccauucg gcgacagcua caucgugaua     1740

ggcguaggag acaagaaaau aacacaccac uggcaccgga guggaagcac gcugggaaaa     1800

gcauucagca caacgcucaa aggcgcccag agacucgcgg cacucggcga caccgcaugg     1860

gacuucggcu caaucggcgg ggucuuuaac agcaucggac gcgcagugca ccaagucuuc     1920

ggaggcgcgu ucagaacccu cuucggaggc auguccugga uaacccaagg ccucaugggc     1980

gcacugcuac uauggauggg ggucaacgcg agagacagaa gcauagccuu agccuuccug     2040

gccaccggag gaguacuggu auuccuagca acaaacgugc acgcgugagg acuaguuaua     2100

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     2160

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10304
<211>  2302
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 1 opt

<400>  10304
gggagaaagc uuaccauggg aaaacggagc gcaggcucca uaauguggcu agcaagccua       60

gccgucguga ucgcgugugc aggcgccguc acccggagag gcucggcgua cuacauguac      120

cucgaccgaa acgacgcagg agaggcaauc ucauucccca caacacucgg aaugaacaaa      180

uguuacaucc aaauaaugga ccuaggccac augugcgacg caacaaugag cuacgagugc      240

ccaaugcucg acgagggagu agaaccagac gacgucgauu gcuggugcaa cacaaccagc      300

accugggucg ucuacggaac cugccaccac aagaaaggcg aagcacgccg aagcaggcgc      360

gcagucacuc uaccgaguca uagcacgcgc aaacuccaaa cacgcagcca aaccuggcuc      420

gagagcagag aauacacaaa gcaccucauc cggguagaga acuggaucuu caggaaccca      480

ggguucgccc uagccgcagc agcaauagca uggcuccuug gaagcagcac cucacagaaa      540

guaauauacc uaguaaugau ucuccucauc gccccagcgu acagcauaag augcaucgga      600

gucuccaaua gagacuucgu agaaggcaug ucaggaggca cauggguaga cguaguccua      660

gaacacggcg gaugcgugac cguaauggca caagacaaac caacggugga uauagagcug      720

gucacaacca caguaagcaa cauggcggaa guccggagcu acugcuacga ggcaucaauc      780

agcgacaugg caagcgacag ucgaugcccc acccaaggcg aagccuaccu cgacaaacaa      840

agcgacacgc aauacguaug caaacgcaca cucgucgaca gggggugggg aaacggcugc      900

ggacucuucg gcaaaggcuc acucguuacc ugcgcaaaau ucgcgugcuc caagaaaaug      960

accggcaagu ccauccaacc ugaaaaccug gaauaccgaa ucaugcuauc gguccacggc     1020

agccaacaca gcggaaugau agugaacgau acaggucacg aaacagacga gaacagagca     1080

aaaguagaaa uaacuccaaa cucaccgcga gcagaagcca cccugggcgg cuucggaagc     1140

cuaggccucg acugcgaacc ccgcaccggg cuagacuuua gcgauuugua cuaccucacc     1200

augaacaaca aacacuggcu cguccacaaa gaaugguucc acgacauacc ccucccaugg     1260

cacgcgggcg cagacaccgg aaccccacac uggaacaaca aagaagccuu aguagaguuc     1320

aaagacgcac acgcaaagcg acaaaccgua gucgugcucg gcucccaaga aggagccgua     1380

cacaccgcac ucgccggggc acuggaagca gaaauggacg gcgcaaaagg aagacucuca     1440

ucaggacacc uuaaaugccg acucaaaaug gacaaacuaa gauuaaaggg agucagcuac     1500

agucucugca cggcggccuu uaccuucaca aaaauccccg ccgaaacacu acacggaaca     1560

gucaccgugg aaguacaaua cgcgggaaca gauggaccau gcaaagugcc agcccaaaug     1620

gcagucgaua ugcaaacccu aacccccgua ggacgauuga uaacagccaa cccagugaua     1680

acagaaagca ccgaaaacag caaaaugaug cuagaacuag aucccccauu cggagacagc     1740

uacauaguca ucgggguagg agaaaagaaa auaacccacc acuggcaccg aagcggcagc     1800

acacugggca aagccuuuuc uacgacgcuc aaaggagcac aacgacucgc agcucucgga     1860

gacacagccu gggacuucgg caguauagga ggcguauuca acucgauagg gagagcaguc     1920

caccaaguau ucggaggagc auucagaaca cuguucggag gcaugagcug gauaacacaa     1980

ggacuuaugg gcgcacuccu acuguggaug ggagucaacg cuagagacag aucaauagcc     2040

cuagccuucu uggccaccgg cggaguacuc guauuccuug caacaaacgu gcacgcguga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2280

ucuuuucaga gccaccagaa uu                                              2302


<210>  10305
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10305
gggagaaagc uuaccaugac auccguggga auagucggac uauuacucac caccgcaaug       60

gccgcaguca cccucccaag ucacucuacc cgcaaacucc aaacccgauc gcagaccugg      120

cucgaaagcc gcgaauacac aaaacaccuc auccgagucg aaaacuggau cuuccgaaac      180

ccaggauucg cccucgccgc cgccgcaauc gccuggcucc ucgggagcuc uacaucacaa      240

aaagucauau accucguaau gauacuacuc auagcccccg ccuauuccau ccgcuguaua      300

ggaguaucaa accgagacuu cguagaaggc augagcggcg gcaccugggu agacaucgua      360

cucgaacacg gaggcugcgu aaccgucaug gcacaagaca aacccaccgu agacauagaa      420

cucgucacaa caaccguauc aaacauggcg gaaguccgcu ccuacugcua cgaagccuca      480

aucagcgaca uggccuccga cucacgcugc ccaacacagg gcgaggccua ccuagacaaa      540

caaagcgaca cccaauacgu augcaaacgc acacucgucg accgaggcug gggaaacggc      600

ugcggccucu ucggaaaagg aagccucgua accugcgcca aauucgccug cuccaagaaa      660

augacuggaa aauccauaca accggaaaac cucgaauaca gaaucaugcu cagcguccac      720

ggcucacaac acagcggcau gaucgugaac gacaccggac acgaaaccga cgaaaaccgc      780

gcaaaaguag aaauaacucc aaacucacca cgcgcugagg ccacacuagg cggcuucggc      840

ucccuagggc ucgacugcga accaagaacc ggucucgacu ucucagaccu cuacuaccuc      900

acaaugaaca acaaacacug gcuuguccac aaagaauggu uccacgacau accccucccc      960

uggcacgccg gcgccgacac aggcacaccc cauuggaaca acaaagaagc ccucguggag     1020

uucaaagacg cgcacgcaaa acggcaaacc guaguagugc uaggguccca agaaggagcc     1080

guccacacgg cccucgcugg agcccucgaa gcagaaaugg acggcgcuaa aggccgacug     1140

uccucaggac accucaaaug ccgccucaaa auggacaaac uacgccucaa agguguuucc     1200

uacucacuau gcaccgccgc cuucacguuc accaaaaucc cagccgaaac ccuccacgga     1260

acagucaccg ucgaagugca auacgccggc accgacggcc ccugcaaagu cccagcccaa     1320

auggcagugg acaugcaaac ccuaacccca gucggccgcc uaauuaccgc caaccccguc     1380

aucaccgaau ccacagaaaa cagcaaaaug augcucgaac ucgacccacc cuucggcgac     1440

ucguacaucg ugauaggcgu cggagaaaag aaaaucacac accacuggca ccgcagcggc     1500

ucaacaauag gcaaagcguu cgaagccacc guccgcggcg ccaaacgcau ggccgugcuc     1560

ggcgacacag ccugggacuu cggauccguc gguggugccc uaaauagcuu gggcaaggga     1620

auccaccaaa ucuucggagc cgccuucaaa agccuuuucg gagggauguc cugguucucc     1680

cagauccuca uaggaacccu acuaaugugg cuaggccuaa acacgaagaa cggcucaaua     1740

agccucaugu gccucgcacu aggcggagua cuaaucuucc ugucgacugc cguauccgca     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10306
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10306
gggagaaagc uuaccaugac cagcgucgga auagucggcc uccuccucac gacagcgaug       60

gcagccguga cacuccccuc ccacucgaca agaaaacuac aaacacgcuc ccaaacgugg      120

cucgaaucca gagaguacac uaaacaccug auccgugucg aaaacuggau auuccgcaac      180

ccaggcuucg cccuggcagc cgccgcaauc gccuggcuac ucggcagcag caccucacaa      240

aaagucaucu aucuaguaau gauacuccug aucgcgccag cuuacuccau ccgcugcaua      300

ggaguaucca acagagacuu cgucgaaggc augagcggag gcacaugggu cgacgucguc      360

cuagaacacg gaggaugcgu caccguaaug gcacaagaua aacccacggu cgacaucgag      420

cuaguuacca caacagucag caacauggcc gaaguccgca guuacugcua cgaagccagc      480

auaagcgaca uggccucaga cagcagaugc ccaacccaag gcgaagccua ccucgacaaa      540

cagucagaca cgcaauacgu augcaaaaga acccucguag aucgaggcug gggcaacgga      600

uguggacucu ucgggaaagg cagccucgua accugcgcca aauucgcgug caguaagaaa      660

augacaggca aauccaucca accagaaaac cucgaauacc guauuaugcu cuccguccac      720

ggcagccagc acuccggcau gaucguaaac gacaccggac acgagaccga cgaaaaccga      780

gccaaagucg aaaucacccc caacucaccc cgcgcagaag ccacacucgg cggcuucggc      840

ucccucggac uggacugcga accaaggaca ggccuagacu ucucagaccu auacuaccuc      900

acaaugaaca acaaacacug gcucgugcac aaagaauggu uccacgacau cccccucccc      960

uggcacgccg gagcagacac cggaacaccc cacuggaaca acaaagaggc ccuuguagag     1020

uucaaagaug cacacgccaa gcgccaaacc guaguuguac ugggaagcca agaaggcgca     1080

guacacaccg cacuagcagg agcacuagaa gccgaaaugg acggcgccaa aggccgccua     1140

uccagcggcc accuaaaaug ccgccucaaa auggacaagc ucaggcugaa aggcgucagc     1200

uacucccucu gcaccgcagc auucaccuuc accaaaauac cugcagaaac ccuccacggc     1260

accgucaccg ucgaaguaca auacgcaggc acggacggcc caugcaaagu acccgcccaa     1320

auggccgugg acaugcaaac ccucacacca guaggccgac ucaucacagc caauccagua     1380

aucacggaaa gcacagaaaa caguaaaaug augcuagaac ucgacccacc cuucggagac     1440

ucuuacaucg ucauaggagu aggcgaaaag aaaaucaccc accacuggca ucgcuccggc     1500

ucaaccaucg gcaaagcauu cgaagccacc guacgcggcg ccaaacguau ggcuguccua     1560

ggagacaccg caugggacuu cggaucgguc ggcggcgccc ucaacagccu aggaaaaggc     1620

auccaccaaa ucuuuggagc ggcguucaaa ucccucuucg gaggaauguc gugguucucc     1680

caaauacuaa ucgguacgcu acuaaugugg cucggccuca acgccaaaaa cggaucaauu     1740

agccucaugu gccuagcacu aggcggaguc cuaaucuucc uaagcacagc gguaagcgcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10307
<211>  1993
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10307
gggagaaagc uuaccaugac cuccaucgga auaauuggcc uacuacucac aaccgcaaug       60

gcggccguaa cccuucccuc acacuccacu cguaaacugc aaacccgcuc ccagacaugg      120

cucgaaucga gagaauacac aaaacaccua aucaaagucg aaaacuggau cuuccgcaac      180

cccggauucg cccuagucgc cgucgcaauc gcauggcucc ucggcuccuc aaccagccaa      240

aaaguuaucu accucguaau gauccuccuc aucgcacccg cauacucaau acgcugcauc      300

ggcgucucua accgcgacuu uguagaagga augucaggcg gcaccugggu cgacgucgua      360

cucgaacacg ggggaugcgu caccgucaug gcccaagaca aaccuacagu cgacaucgag      420

cuagucacga ccacagucuc aaacauggca gagguaagaa gcuacugcua cgaagccucc      480

aucuccgaua uggccuccga cucccgaugc cccacacagg gcgaagcaua ccuagacaaa      540

caauccgaca cccaauacgu cugcaaacgc acacucguag accguggcug ggggaacggc      600

ugcggacuau ucggaaaggg cucccucguc acaugcgcga aauuuaccug cuccaagaaa      660

augaccggaa aaagcauaca gcccgaaaac cucgaauacc gcaucaugcu cuccgugcac      720

ggcagucaac acucaggcau gauaggcuac gaaacagaug aagaccgcgc aaaggucgaa      780

gucaccccaa acucccccag agccgaagcc acccucgggg gcuucggcag ccucggacua      840

gacugcgaac cuagaaccgg ccuagacuuc agcgaccucu acuaccuaac gaugaacaac      900

aaacacuggc uuguacauaa agaaugguuc cacgacaucc cccugcccug gcacgccgga      960

gccgacacag gaacaccgca cuggaacaac aaagaagcac ucguugaguu caaagacgcc     1020

cacgcaaaac gccaaacagu cgucguacua ggaagccaag aaggcgccgu ccacaccgca     1080

cuagcaggag cauuagaagc cgaaauggac ggcgcaaaag gccgccucuu cuccggccac     1140

cucaaaugcc gacuaaaaau ggauaaacuc cgccucaaag gcgucagcua cucacucugc     1200

accgccgccu ucacauucac caaagucccc gccgaaacac uacacggcac cguaacuguc     1260

gagguacaau acgccggaac ggacggcccu ugcaagauac ccguacaaau ggcaguggac     1320

augcaaacac ucacccccgu cggcagacua auaacagcca acccgguaau aaccgaaagc     1380

accgagaacu cuaaaaugau gcuagaacuc gacccacccu ucggcgacag cuacaucguc     1440

aucggcgucg gcgacaagaa gaucacacac cacuggcaca gaucagguuc cacaaucggc     1500

aaggcauucg aagccacggu ccgcggcgca aaacgaaugg ccguccuagg cgauaccgcc     1560

ugggacuucg gcuccgucgg aggagucuuc aacucccucg gaaaaggaau ccaccaaauc     1620

uuuggagccg ccuucaaauc gcuauucggc ggcaugagcu gguucagcca gauccuaaua     1680

gguacacugc ucguauggcu cggcuuaaau accaaaaacg gaagcaucuc ccuaacgugc     1740

cucgcccucg gcggcgucau gaucuuccuc uccacagccg ugagcgcaug aggacuaguu     1800

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     1860

uuaauaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaau gcaucccccc cccccccccc cccccccccc cccccaaagg cucuuuucag     1980

agccaccaga auu                                                        1993


<210>  10308
<211>  2005
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10308
gggagaaagc uuaccaugac aagcguaggc auagucgggc uccuacuuac aacagccaug       60

gcagccguaa cauuaccauc acacuccacc cgcaaacuac agaccagauc ccaaaccugg      120

cucgaaagcc gcgaauacac aaaacaccuc auacgcgucg aaaacuggau cuucagaaac      180

cccggguuug cccuagcagc cgcagcaaua gccuggcugc ugggaagcuc cacaucacaa      240

aaggucaucu accuaguaau gauccugcuc auagccccag ccuacuccau ccgcugcaua      300

ggcgucagca accgcgacuu cguagaaggg augucaggcg guacaugggu agacgucgua      360

cucgaacacg gaggaugcgu caccguaaug gcgcaggaca aaccaacagu agacaucgaa      420

cucgucacaa ccaccgucuc gaacauggcc gaagugcgaa gcuacugcua ugaagcaucc      480

aucucagaca uggcguccga cagccgaugc cccacccaag gagaggcaua ucucgacaaa      540

caaagcgaca cccaauaugu gugcaaacgc acccucgugg accgcggaug gggaaacgga      600

ugcggccugu ucggaaaagg cucacucgua acaugcgcaa aauucgcaug cagcaagaaa      660

augaccggaa aaagcauaca gccugaaaac cuagaauaca gaauaaugcu gagcguccac      720

ggcucacaac acuccggcau gauaguaaac gacacaggcc acgaaaccga cgaaaacaga      780

gccaaaguag aaaucacacc aaauucccca cgagcagaag ccacacucgg cggcuucggc      840

agccuaggcc uagacugcga accacggaca ggacuagacu ucagcgaccu cuacuaccuc      900

acaaugaaca acaaacacug gcugguccac aaagaauggu uccacgauau accccucccc      960

uggcacgccg gagcagacac cggcacccca cacuggaaca acaaagaagc acuggucgag     1020

uucaaagacg cucacgcgaa acgccagaca gucgucguac ugggcagcca agaaggcgcc     1080

guccacaccg cucucgccgg cgcacuagaa gcggaaaugg acggggcaaa aggccggcua     1140

ucaagcggcc accugaaaug ccgacucaaa auggacaaac uacgacugaa gggcgucagc     1200

uacagucuau gcacagccgc auucaccuuc acaaaaaucc ccgcagaaac ccuacacggc     1260

acagucaccg ucgaagugca auacgccggc acagacggac ccugcaaagu ccccgcucag     1320

auggccgucg acaugcaaac auuaaccccc gucggacgac uaauaaccgc aaacccagua     1380

aucaccgaau ccacagagaa cuccaaaaug augcucgaac ucgauccccc auucggcgac     1440

agcuacauag uaaucggcgu aggagaaaag aaaauaacgc accacuggca ccgaagcggc     1500

uccaccauag gcaaagcauu cgaagcgacc guccgcggag ccaaacgcau ggcagucuua     1560

ggcgacaccg caugggacuu cggcuccguc ggcggcgccc uaaacagccu cggaaaagga     1620

auccaccaaa ucuucggcgc cgcauucaaa agccuauucg ggggcauguc augguucucc     1680

caaauccuga uaggaacccu acucaugugg cuagggcuga acaccaaaaa cggcagcaua     1740

agccuaaugu gccuagcacu aggcggaguc cucauauuuc ucucaacagc cgucucggcc     1800

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     1860

cuugcaccga gauuaauaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     1980

ggcucuuuuc agagccacca gaauu                                           2005


<210>  10309
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10309
gggagaaagc uuaccauggc cauaucaggc gucccugucc ucggcuucuu caucaucgcc       60

guacuaaugu ccgcccaaga gucaugggcc gcagugaccc ucccaagcca cagcacgcgc      120

aaacuacaaa cccgcagcca gaccuggcuc gaaagccgag aauacaccaa acaccuaaua      180

agagucgaga acuggaucuu ccgcaacccc ggauucgcgc ucgcggccgc cgccaucgca      240

uggcuccuag gcagcuccac cucacaaaaa gucauauacc ucgugaugau ccuccuuauc      300

gcccccgccu acuccauccg augcaucgga gucagcaacc gagacuucgu cgaaggaaug      360

ucaggcggca caugggucga cauaguccuc gaacacggcg gaugcgucac cguaauggcc      420

caagacaaac ccacagucga caucgagcua gucacaacca caguaucaaa cauggcagaa      480

guaagaagcu acugcuacga agccuccauc ucagacaugg ccucggacuc acgaugcccc      540

acgcagggag aagccuaccu ggacaaacag ucugacacac aauacgucug caaaagaacc      600

cucguagacc gcggaugggg caacggcugc ggacuauucg gaaagggcuc ccuaguaacc      660

ugcgcaaaau ucgcaugcuc gaagaaaaug accggcaaau caauucaacc cgagaacuua      720

gaauaccgca uaaugcucuc aguacacggc ucacaacaua gcgggaugau cguuaacgac      780

accggccacg aaacagacga aaaccgagcg aaagucgaaa uaacaccaaa cagcccacgc      840

gcggaagcca cccugggcgg cuucgggucc cucggcuuag acugcgaacc gcgaacagga      900

cuagacuuua gugaccucua cuaccucacc augaacaaca aacacugguu aguacauaaa      960

gaaugguucc acgacauccc acugcccugg cacgccggcg ccgacaccgg cacaccccac     1020

uggaacaaua aagaagcccu cguagaguuc aaagacgccc acgcgaaacg acaaacaguc     1080

gucguacuag gcagccaaga aggcgcuguc cacaccgcuc ucgccggagc acucgaagcc     1140

gaaauggaug gagcgaaagg acgacuaagc uccggccacc ucaagugccg acucaaaaug     1200

gacaagcucc gccugaaagg cgucaguuac ucccuuugca cugcagcauu uaccuuuacg     1260

aaaauacccg ccgaaacucu acacggaacc gucacaguag aaguccaaua cgccggcaca     1320

gacggaccau gcaagguccc agcccaaaug gccgucgaca ugcaaacacu uaccccagua     1380

ggaagacuaa uaacagccaa ucccguaauc acagaaucua ccgagaacuc aaaaaugaug     1440

cucgagcucg aucccccauu uggcgacagc uacaucguca uaggggucgg cgaaaagaaa     1500

aucacacacc acuggcaccg cagcggcagc acaauaggca aagcauucga agccacaguc     1560

agaggcgcaa aacguauggc gguacugggc gacacagccu gggacuucgg aaguguaggc     1620

ggagcacuaa acucguuagg caaaggcaua caccaaauau ucggagcagc cuucaaaagc     1680

cuguucggcg gaaugucaug guucucgcaa auccuaaucg gcacucuccu caugugguua     1740

ggccucaaca ccaaaaacgg gagcaucagc cuaaugugcc ucgcacucgg aggagugcuc     1800

aucuuccuaa gcacggcagu cuccgccuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10310
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10310
gggagaaagc uuaccauggc caucagcggc guccccguac ucggauucuu caucaucgcc       60

guacuaaugu ccgcccaaga aagcugggca gccgugaccu uaccaucaca cucaacuaga      120

aaacuccaaa cacgcuccca aaccuggcuc gaaucuagag aguacaccaa acaccucauc      180

cgaguagaaa acuggaucuu ccgaaaccca ggauucgcac uugccgcagc cgcgaucgca      240

uggcugcucg gaagcucuac cucacaaaaa guaauauacc uagucaugau ucuccuuauc      300

gcccccgcau acagcauacg cugcaucgga gucuccaaca gagacuucgu cgaaggcaug      360

agcggcggca cauggguaga cguaguccuc gaacacggag gaugcgucac cguaauggcc      420

caagacaaac ccaccguaga cauagaacug gucacaacaa ccgucucaaa cauggccgag      480

guaaggagcu acugcuacga agccuccauc uccgacaugg caagcgacag ucgcugccca      540

acccaaggag aagccuaccu agacaagcaa agugacaccc aauacguaug caaacgaacc      600

cucgucgacc gaggaugggg aaacgggugc ggccuauucg gaaaaggaag ccucgucacg      660

ugcgcaaaau ucgcaugcag caagaaaaug acaggcaaaa gcauccaacc cgaaaaccuc      720

gaguacagaa uaaugcuaag cgugcacggc ucacaacacu ccggcaugau cguaaacgac      780

acaggacacg aaaccgacga gaaccgagcc aaagucgaaa ucacccccaa cucaccacgc      840

gccgaagcaa cccucggcgg auuugguucc cucggacuag acugcgaacc aagaaccgga      900

cucgacuucu ccgacuuaua cuaccuaacc augaacaaca aacacuggcu aguacauaaa      960

gaaugguucc acgacauccc acuacccugg cacgcgggcg cggacaccgg caccccacac     1020

uggaacaaca aagaagcacu cguagaauuu aaagacgcac acgccaaacg ccaaacagug     1080

guagugcuag gaucccagga aggagccguc cacaccgcac ucgcaggggc ccuugaagcc     1140

gaaauggacg gagcgaaagg ccgccuuucg agcggacacc ucaagugccg ccuaaagaug     1200

gacaaacucc gacucaaggg aguaagcuac ucccucugca cagcagcauu cacauucaca     1260

aaaauacccg ccgagacacu ucacggcacc gucaccgucg aaguccagua ugccggaacc     1320

gacggacccu gcaaaguacc agcucaaaug gccguagaca ugcaaacccu aacgcccguc     1380

ggacgacuaa uaacagcgaa cccggucauc accgaaucaa ccgaaaacag caaaaugaug     1440

cucgaacuag aucccccauu cggagacagc uacauaguaa ucggcguagg agaaaagaaa     1500

aucacgcacc acuggcaccg cucaggauca accauaggaa aagcguucga agcaaccgug     1560

cgcggcgcua aacgcauggc aguccucggc gacaccgccu gggacuuugg aucagucgga     1620

ggagcccuca acagccucgg caaaggcaua caccaaaucu ucggagcagc cuuuaaaucc     1680

cucuucggag gcaugucgug guucagccaa auacuaaucg ggacccuacu aauguggcua     1740

ggacucaacg ccaaaaacgg cagcauaucc cucaugugcc uagcccucgg cggaguccuc     1800

auauuucuau caacagccgu cucagcuuga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10311
<211>  2020
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10311
gggagaaagc uuaccauggc gauauccgga gucccagucc ucggcuucuu caucaucgca       60

guccugaugu cagcgcaaga aagcugggca gcggucacac uacccagcca cuccacacgc      120

aaacuccaaa cacgcagcca gacauggcua gaaucccgcg aauacacaaa acaccucauc      180

aagguagaaa acuggaucuu ccgaaacccc ggauucgcgc ucgucgcugu agcaaucgca      240

uggcuacucg gcagcucgac aucgcaaaaa gucaucuacc uagucaugau ccuacucauc      300

gcacccgccu acuccauaag augcaucggc gucuccaacc gagacuucgu agaaggaaug      360

ucaggcggca ccugggucga cgucguccua gagcacggag gcugcguaac cgucauggca      420

caggacaaac caacagucga cauagaacuc guaacaacca ccguaucaaa cauggccgaa      480

guccguucau acuguuacga agccucaaua agugacaugg ccucagacag ccgaugcccc      540

acccaggggg aggcauaccu ggacaagcaa ucagacaccc aauacgucug caaaagaacg      600

cucguagacc gcggcugggg uaacggcugc gggcuauucg gcaaaggcuc ccucgucacg      660

ugcgccaaau ucacgugcag caagaaaaug accggaaaaa gcauccaacc ugaaaaccuc      720

gaauaccgaa uaaugcuguc cguccacggc agccaacaca gcggcaugau cggcuacgaa      780

accgacgaag accgcgcaaa agucgaaguc acacccaacu caccacgcgc ggaagccaca      840

cugggaggau ucggaagccu aggacuagac ugcgaaccac gaacaggacu cgacuucagc      900

gaccuuuacu accucaccau gaacaacaaa cacugguuag uccacaaaga augguuccac      960

gacauccccc uccccuggca cgccggagcc gacacaggaa cgccccacug gaacaacaaa     1020

gaagcccucg ucgaguucaa agacgcgcac gcaaaaaggc aaaccgucgu cguucuagga     1080

ucacaagaag gcgcgguaca caccgcgcua gccggcgccc uagaagccga aauggacggu     1140

gccaaagguc gccucuucuc aggacaccuc aaaugccggc ugaaaaugga caaacuccgc     1200

cuaaaaggcg uauccuacag ccucugcacc gcagccuuca ccuucacaaa agucccugcc     1260

gaaacccugc acggcaccgu caccgucgaa guccaauacg ccggaacgga uggccccugu     1320

aaaauccccg uacaaauggc cguagacaug caaacccuca cgcccguagg ccgacucauc     1380

accgccaacc cagucauaac agaauccacc gaaaauagca agaugaugcu agaacucgau     1440

cccccauucg gagacucaua cauagucauc ggagucggcg acaaaaagau aacacaccac     1500

uggcaccgcu caggcucaac caucggcaaa gccuucgaag ccaccguccg cggcgcaaaa     1560

agaauggcag uccucggaga caccgcaugg gacuucggcu ccguaggagg cguauucaau     1620

agccuaggca agggcauuca ccaaauauuc ggggcagccu ucaagagcuu auucggcggg     1680

augucauggu ucucccaaau acucaucgga acacuccuag uguggcucgg ccucaacaca     1740

aagaacggcu caaucagccu uaccugccuc gcacucggag gcgugaugau auuccuaucc     1800

acagccguau ccgccugagg acuaguuaua agacugacua gcccgauggg ccucccaacg     1860

ggcccuccuc cccuccuugc accgagauua auaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaugca uccccccccc cccccccccc     1980

cccccccccc ccaaaggcuc uuuucagagc caccagaauu                           2020


<210>  10312
<211>  2032
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10312
gggagaaagc uuaccauggc aauauccggc gugcccguac ucggauucuu caucaucgcc       60

guccucaugu cagcgcaaga auccugggca gccguaacac uccccuccca cagcacccgc      120

aaacuacaaa caagaagcca aaccuggcua gaaucacgag aauacaccaa gcaccuuaua      180

agagucgaaa acuggauauu ccgaaaccca ggauucgcac uggcggccgc agcaauagca      240

uggcuccuag gcucgaguac aagccaaaaa guaaucuacc ucguaaugau ccuacuaauc      300

gcacccgcgu acucaaucag augcaucggc gucagcaaca gagacuucgu agaaggaaug      360

uccggaggga cuugggucga cguaguacua gaacacggcg gcugcgucac aguaauggca      420

caagacaaac caaccgucga cauagagcua gugaccacaa ccgucagcaa cauggcggaa      480

guacgcagcu acugcuacga agcaagcaua ucggacaugg caagcgacag ccgcugccca      540

acacagggcg aagcauaccu agacaaacaa uccgacaccc aauacguuug caagcgaaca      600

cucgucgacc gcggcugggg caacggcugc ggccuauucg gcaaaggcuc ccuaguaaca      660

ugcgcaaaau ucgcaugcuc uaagaagaug acaggcaaga gcauacaacc agaaaaccuc      720

gaauacagga ucaugcuauc gguccacggu ucacaacauu caggcaugau cgucaaugac      780

acgggacacg aaaccgacga aaaccgcgcc aagguggaaa uaacgcccaa caguccccgc      840

gccgaggcaa cccugggcgg uuucggcagc cucggccucg acugcgaacc aagaaccgga      900

cucgacuuca gcgaccuaua cuaccuaacu augaacaaca agcacuggcu uguccacaaa      960

gagugguucc acgacauacc ccuccccugg cacgcaggag cagacaccgg aaccccacac     1020

uggaacaaca aagaagcacu cguagaguuc aaagacgccc acgcgaaaag acaaacaguc     1080

gugguacugg ggagccaaga aggugccguc cacacagccc uagccggagc acucgaagca     1140

gaaauggacg gcgcaaaagg gcgccucuca ucaggccacc uaaagugccg acuaaaaaug     1200

gacaaacucc gccuaaaagg cguaucuuac ucccuaugca ccgccgcauu caccuuuaca     1260

aagauaccgg ccgaaacccu ccacggcacc guaacaguag aaguccaaua cgccggcacc     1320

gacggccccu gcaaaguccc cgcccaaaug gcaguagaca ugcaaacacu aacacccguc     1380

ggccgacuca uaaccgccaa ccccgucaua acagaaagua cugaaaacuc caaaaugaug     1440

cucgaacucg acccacccuu cggagacagc uacauaguca uaggcgucgg cgagaaaaag     1500

auaacccacc acuggcaccg cuccggcucc acaauaggca aggccuucga agccaccgua     1560

agaggcgcaa aacgcauggc aguccuggga gacacagcau gggacuucgg cuccguugga     1620

ggggcacuaa acucacuagg gaaaggcaua caucaaauuu ucggcgcagc cuucaaaagc     1680

cuauucggcg gaaugagcug guucagccaa auacuaaucg gcacacuacu uauguggcuc     1740

ggccucaaca cgaaaaacgg aagcaucucc cuaaugugcc uagcacucgg cggcgugcua     1800

auauuccuaa gcacagcagu cagcgcauga ggacuaguua uaagacugac uagcccgaug     1860

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaug cauccccccc     1980

cccccccccc cccccccccc ccccaaaggc ucuuuucaga gccaccagaa uu             2032


<210>  10313
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10313
gggagaaagc uuaccaugga cuggacaugg auacuauucc ucguagcagc agcgacaaga       60

gugcacagug caguaacccu ccccucacac agcacgagaa aacuacagac gcgcagccaa      120

accuggcucg aaucacgaga auacaccaaa caccuaauac gagucgagaa cuggauauuc      180

agaaaccccg gcuucgcccu agcagcagca gcaaucgccu ggcuccuagg cagcagcaca      240

ucacaaaaag ugauauaccu gguaaugauc cuucuuauag cccccgccua uaguauaaga      300

ugcaucggcg ucagcaaucg cgacuucguc gaaggaaugu cgggaggaac cugggucgac      360

auaguucuag agcacggagg cugcgucacc guaauggcac aagacaaacc aacaguggac      420

aucgaauuag uuaccacaac gguaagcaac auggccgaag ucagaagcua uugcuacgaa      480

gcaagcauau cagacauggc aagcgacucc cgaugcccca cccaaggcga ggcuuaccua      540

gacaaacaga gugacaccca guacgugugc aaaagaaccc uagucgaccg uggcuggggc      600

aacggaugcg gacucuucgg caaagggagc cucguaacau gcgcaaaauu cgcuugcuca      660

aagaaaauga ccggcaaaag cauccaacca gaaaaccuag aauaccgaau caugcucucc      720

guacacggau cacaacacag cggaaugaua gugaacgaca ccgggcacga gacagacgag      780

aaccgcgcca aaguagaaau aacccccaac agucccagag ccgaagcgac acuaggagga      840

uucggaucau uagggcugga cugcgagcca cgaaccggcc uagacuucuc agaccuauac      900

uaccucacga ugaacaacaa acacuggcuc guacacaagg aaugguucca cgacauaccc      960

cuaccauggc acgccggcgc agacacaggc acaccccacu ggaacaauaa agaagcccuc     1020

gucgaauuua aagacgccca cgccaaacgc caaacagugg ugguacucgg cucacaagaa     1080

ggugccguuc acacggcccu agcaggcgcc cuggaagccg aaauggacgg agccaaaggu     1140

cgacuaagca gcggacaucu aaaaugcaga cugaaaaugg acaaacuccg gcucaaagga     1200

guaucauaca gccuaugcac cgccgccuuc acguucacaa aaauaccagc agagacccuc     1260

cacggcacag ucacgguaga aguccaauac gccggcaccg acggccccug caaagugcca     1320

gcacaaaugg cagucgacau gcaaacccug acacccgucg gcaggcuaau caccgcaaac     1380

cccgucauca cagagaguac agagaacagc aaaaugaugc ucgaacucga cccacccuuc     1440

ggagacucgu acaucgucau aggcgucggc gaaaagaaaa ucacccauca cuggcauaga     1500

agcggaagca ccauaggcaa agccuucgaa gccaccguac gcggggccaa acgcauggcc     1560

guccucggag acacagcgug ggacuucggc ucgguaggcg gcgcacucaa cucacucggc     1620

aaaggcauac accaaaucuu cggcgcagcc uucaaauccc uauucggcgg gaugagcugg     1680

uucucgcaaa uacuaauagg cacccugcua auguggcucg gacuaaacac caaaaacggc     1740

ucgaucucau uaaugugccu agcgcucggc ggaguacuaa ucuuccuauc aacagcagua     1800

ucagccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10314
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10314
gggagaaagc uuaccaugga cuggaccugg auccucuucc ucguagccgc cgccacacgc       60

guacacagcg cagucacacu cccgagccac agcaccagaa aguuacaaac acgcucucaa      120

acguggcuag agucccgaga auacacaaaa caccuaauac gcguagaaaa cuggaucuuc      180

agaaacccag guuucgcccu ggcggcagcc gccaucgcgu ggcuacucgg cucaagcacc      240

agccaaaaag ucaucuaccu cgucaugauc cuacucauag ccccagccua cuccauccga      300

ugcaucggag ucuccaauag agacuucgug gagggaauga gcggcgguac cugggucgac      360

guuguccuag aacacggcgg cugcgucacc guuauggccc aagacaaacc caccgucgac      420

auagaauuag ucaccacaac agucucaaac auggccgaag uccgaagcua cuguuacgaa      480

gccuccaucu ccgacauggc cagcgacuca cggugcccga cccaaggcga ggcuuaccuc      540

gacaaacaau cagacacaca auacgucugc aaacgcacac ucgucgacag aggcuggggc      600

aacggcugcg gccuguucgg caaaggcucc cuaguuaccu gcgcuaaauu cgcaugcagc      660

aagaaaauga ccggcaaaag cauccagccc gaaaaccuag aauacagaau caugcucuca      720

guccacggaa gccaacacag cggcaugaua gucaacgaca ccggccacga aaccgacgaa      780

aacagagcca aagucgaaau cacccccaac ucaccccgcg cggaggccac acuuggcgga      840

uucggcuccc uaggacugga cugcgaaccc cgcaccgggc uagacuucuc cgaccuauac      900

uaccucacaa ugaacaacaa acacuggcua guccacaaag aaugguucca cgacauacca      960

uuacccuggc augccggcgc agacacagga acaccccacu ggaacaacaa agaagcacuc     1020

gucgaguuca aagacgccca cgccaaacgc caaaccgucg ucgugcuagg aucccaagaa     1080

ggugccguac acaccgcccu ugcaggggcu cucgaagccg aaauggacgg cgccaaggga     1140

cgccuuuccu ccggccaccu gaaaugccgg cucaaaaugg acaaauuacg acucaagggu     1200

guaucauacu cccucugcac cgcagcauuc accuucacca aaauccccgc cgaaacgcuc     1260

cauggcaccg ucacagucga aguucaauac gccggcaccg acggacccug caaggugccg     1320

gcgcaaaugg ccgucgacau gcaaacccua acgcccguag ggaggcucau cacugccaac     1380

ccaguaauaa cagaaagcac agaaaacucc aaaaugaugc ucgaacucga cccgcccuuc     1440

ggggacucau acauaguaau uggcguaggc gaaaagaaaa uaacacacca cuggcacaga     1500

agcggaucaa caauaggaaa agccuucgaa gcaacgguaa gaggggccaa aagaauggcc     1560

guacucggag acaccgccug ggacuucggc ucaguugggg gugcacuaaa cucccucggc     1620

aaagguaucc accaaauauu cggcgcugca uucaaaucac ucuucggagg aaugucaugg     1680

uucucccaga uacucauagg cacccugcuc auguggcucg gccucaacgc gaaaaacggu     1740

agcauuucac uaaugugccu cgcauuaggc ggagugcuca uauuccuauc aaccgcaguc     1800

agcgccugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10315
<211>  1999
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10315
gggagaaagc uuaccaugga uuggaccugg auacucuucc uagucgcagc agcaaccaga       60

guacacucag ccguuacccu ccccagccac ucaacaagaa agcugcaaac acgcagccaa      120

acguggcuag aaucccgcga auacacgaaa caccucauaa aggucgaaaa cuggaucuuc      180

agaaacccag gcuucgcgcu cgucgcgguc gccaucgcau ggcuacucgg guccagcacu      240

ucccaaaaag ucauauaccu agucaugauc cuacucaucg cacccgcuua cagcauccgc      300

ugcaucggag uauccaaucg agacuucgua gaaggcauga gcggaggcac auggguagac      360

guaguccucg aacacggcgg augcgucacc guaauggcgc aagacaaacc caccguagac      420

aucgaacucg uaacaacaac cgucucgaac auggcagaag ucagaagcua cugcuacgaa      480

gcaagcauaa gcgacauggc gagcgacucc cgcugcccca cccaagggga agccuaccug      540

gacaaacaaa gcgacaccca auacguaugc aaacgaacac ucguagauag aggcuggggc      600

aacggaugcg gccuauucgg aaagggaucc cuagucacau gcgcaaaauu cacaugcuca      660

aagaaaauga caggcaaauc aauacaaccc gaaaaccucg aauaccgcau caugcucagc      720

guccacggau cacaacacuc gggaaugaua ggauacgaaa cggacgaaga ccgggcaaaa      780

guagagguca caccaaacuc cccaagggcg gaagccaccc ucggcgguuu cggcucacug      840

gggcuagacu gcgaaccccg caccggacuc gauuucucag accuauacua ccucacaaug      900

aacaacaaac acuggcucgu gcacaaggaa ugguuccacg acauaccccu cccguggcac      960

gccggcgcgg acacaggaac accccacugg aacaauaaag aagcacucgu cgaguucaaa     1020

gacgcacacg ccaaacgcca gaccguagua guacucggau cacaagaagg ggccguacac     1080

acggcgcugg ccggagcacu agaagcagaa auggauggcg caaaaggccg gcuauucagu     1140

ggacaccuaa aaugucgccu aaaaauggac aaacucagac ucaaaggagu cuccuacucc     1200

cuaugcacgg ccgcguucac auucacaaaa guccccgcag aaacacucca cggcacgguu     1260

acagucgaag uccaauacgc aggcaccgac ggccccugua aaauaccugu acaaauggcc     1320

gucgacaugc aaacacucac acccgucgga agacugauca cugcaaaccc agucauaaca     1380

gaaaguacag aaaacagcaa aaugaugcua gaacuugacc cacccuucgg cgauucguac     1440

aucgucaucg gcguaggaga caagaaaauc acccaccacu ggcaccgaag cggcagcacc     1500

auaggaaaag cauucgaagc aacaguacgc ggagccaaaa gaauggccgu ccuuggagac     1560

acagccuggg acuucggguc cgucggaggc guauucaaca gccugggaaa aggcauccac     1620

cagaucuucg gcgcagcauu caaaucgcua uucgguggaa uguccugguu cagccaaauc     1680

cuaaucggga cccucuuagu guggcuagga cuaaacacga aaaacggcag caucagccua     1740

accugccuag cccugggagg ggucaugauc uuccucucaa cugcagucuc agcaugagga     1800

cuaguuauaa gacugacuag cccgaugggc cucccaacgg gcccuccucc ccuccuugca     1860

ccgagauuaa uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     1980

uuucagagcc accagaauu                                                  1999


<210>  10316
<211>  2011
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10316
gggagaaagc uuaccaugga cuggacgugg auacucuucc ucguagcagc ggccacaaga       60

guccacagcg caguaacacu gcccucacac ucuacccgca aacugcaaac acgcagccaa      120

acauggcuag aaucacgcga guacacaaaa caccugaucc gagucgaaaa cuggauauuc      180

agaaacccag gauucgcccu ggcagcggcc gccauagccu ggcuacucgg aagcucuacc      240

ucacaaaagg uaauauaccu aguaaugauc cuccucaucg cccccgccua cucaauacgc      300

ugcaucggag uaagcaaccg cgacuucguc gaaggaauga gcggaggaac cuggguggac      360

gucgucuuag agcacggagg augugucacg guaauggccc aagacaaacc caccgucgac      420

auagaguugg ucaccacaac agucuccaac auggccgagg ucagaagcua cuguuacgaa      480

gcaaguaucu cagacauggc cagcgacagc cgcugcccaa cacaaggaga agcauaccuc      540

gacaagcaaa gugacacaca auacgugugc aagcgcacgc uaguagaccg aggcugggga      600

aacggcugcg gccucuucgg aaaaggcagc cucguaacau gcgccaaauu cgccugcucc      660

aaaaagauga ccggcaaguc gauccaaccc gaaaaccucg aauacagaau caugcuaucc      720

guacacggaa gccaacacuc aggcaugaua gucaacgaca cuggacacga aaccgacgaa      780

aaccgcgcca aaguagaaau cacaccaaac ucaccacgag cagaagcaac cuuaggagga      840

uucggcuccc uaggacucga uugcgagccc agaacagggc uggacuucuc agaccuauac      900

uaccuaacaa ugaacaacaa acacuggcua guacacaaag aaugguuuca cgacauacca      960

cuaccauggc acgcuggugc agacacggga acgccccacu ggaacaacaa agaagcacuc     1020

guagaguuca aagacgcaca cgcaaagaga caaacaguug ucguccuagg aagccaagaa     1080

ggcgccguac acacggcccu cgccggagcu cucgaagccg aaauggacgg agcuaaggga     1140

agacucagca gcggccaccu aaaaugccga cuaaaaaugg acaaacuccg acucaaggga     1200

guaagcuacu cacuaugcac ggccgccuuc acauucacaa aaauacccgc cgagacccuc     1260

cacggaaccg ucaccgucga gguacaguac gcaggaaccg acggacccug caaaguaccc     1320

gcgcagaugg caguagacau gcaaacacuc acccccgugg gacgccucau aaccgcgaac     1380

ccagucauaa ccgaguccac ggaaaacagc aaaaugaugc uagaacuuga cccaccauuc     1440

ggagacuccu acaucguaau aggcgucggc gaaaagaaaa ucacacacca cuggcaccga     1500

ucaggcucga ccaucggaaa agccuucgaa gcaacaguac gcggcgccaa acgcauggca     1560

guccucggag acacggccug ggacuucggc ucaguagggg gcgcacucaa cagucuagga     1620

aaaggcauac accagaucuu uggggcagcc uucaaaagcc uauucggcgg aaugagcugg     1680

uucagccaaa ucuuaaucgg aacacuacua auguggcucg gccuaaacac caaaaacgga     1740

uccaucagcc uuaugugccu ugccuuagga ggcgugcuaa uauuccugag cacagcugua     1800

agcgcaugag gacuaguuau aagacugacu agcccgaugg gccucccaac gggcccuccu     1860

ccccuccuug caccgagauu aauaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     1980

cccaaaggcu cuuuucagag ccaccagaau u                                    2011


<210>  10317
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 1 opt

<400>  10317
gggagaaagc uuaccauggg uaaaagaucc gccggcagca uaauguggcu ggccucccuc       60

gccgucguga uagcaugcgc aggcgccgca gucacacucc ccagccacag caccagaaaa      120

cuacaaaccc gcucccaaac cuggcuagaa ucaagagaau auacaaaaca ccucauaaga      180

gucgaaaacu ggauauuccg aaaccccggc uucgcacucg ccgccgcagc aauagccugg      240

cuccucggcu cuagcacguc acaaaagguc auauaucuag ucaugaucuu gcuaaucgcc      300

ccagcauacu ccauccggug cauaggcguu uccaaucggg acuucguaga aggcaugucc      360

ggcggaaccu gggucgauau aguccuagaa cauggcggau gcgucacagu cauggcacaa      420

gacaaaccca ccguagacau agagcuaguc accacgaccg ugagcaacau ggccgaaguc      480

agaucauacu gcuacgaagc aucaaucucg gacauggcau ccgacagccg cugucccaca      540

cagggagaag ccuaccuaga caaacaaucc gacacacaau acgucugcaa acgaacccua      600

guagaccgcg guuggggaaa cggaugcggc cucuucggca aaggaagccu ggucacaugc      660

gcaaaauucg ccugcagcaa gaagaugacc ggcaaaagca uccaaccuga aaaccucgaa      720

uaccgcauca ugcuuuccgu acacggcagu caacacuccg gaaugaucgu caacgacaca      780

gggcacgaaa ccgacgaaaa ccgcgccaaa gucgaaauca cacccaacag cccccgagcc      840

gaagccaccc ucggcggcuu cggcucccuc ggacuagacu gcgagcccag gacgggccuc      900

gacuucuccg accuauacua ccucacaaug aacaacaaac acuggcuagu ccacaaagaa      960

ugguuccacg acaucccccu ccccuggcac gcaggcgccg acacaggcac accacacugg     1020

aacaacaagg aagcacucgu agaguucaaa gacgcccacg caaaacgcca aacaguagua     1080

guacugggau cccaagaagg agccgugcac accgcacuag caggcgcacu cgaagccgaa     1140

auggacggag cgaagggccg acuaucgucc ggacaccuua aaugcaggcu gaagauggac     1200

aaacucagac uaaaaggagu auccuacucc cuaugcacag ccgcauucac auucaccaaa     1260

auacccgcag aaacccuaca cggaaccguc acagucgaag uccaauacgc cggaaccgac     1320

ggccccugca aaguccccgc gcaaauggcc guagacaugc aaacucucac ccccguugga     1380

agacucauca ccgccaaccc ggucaucacg gaaaguaccg aaaacuccaa aaugaugcuc     1440

gagcuagacc cacccuucgg agauuccuac auagucaucg gcguaggaga aaagaaaaua     1500

acacaucacu ggcacagauc cggcuccacc auaggcaaag ccuucgaagc caccguacgc     1560

ggggcuaaac gcauggccgu ccucggcgac acagcauggg acuucggauc cguaggcgga     1620

gcccucaacu cacugggcaa agggauucac caaaucuucg gcgccgccuu caaaagccua     1680

uucggcggaa ugagcugguu cucccaaauc cuaauuggca cccuacuaau guggcucggc     1740

cucaacacga aaaacggcag caucagccuu augugcuuag cccucggagg cguccucauc     1800

uuccucucca cagccgucuc cgcaugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10318
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 1 opt

<400>  10318
gggagaaagc uuaccauggg gaaacgaagc gcaggcucca ucauguggcu cgccucacua       60

gcagucguca uagcaugcgc aggcgcagcc guaacccucc cuucccacuc aacaagaaaa      120

cuacaaacca gaucacaaac guggcuagaa ucgcgagaau acacgaaaca ccucaucaga      180

gucgaaaacu ggauauuuag gaaccccggc uucgcccuag cagcagccgc aaucgcgugg      240

cugcugggca gcucaacauc gcaaaaagug auauaccuag uaaugauccu ccuaauagcc      300

ccggcauaca gcauacgcug caucggagua ucaaaccgag acuuugucga ggguaugucc      360

ggcggaacau ggguagacgu gguucucgaa cacggcggcu gcguaaccgu aauggcacaa      420

gacaaaccaa ccguggacau cgaacucguc acaacaaccg uaagcaacau ggccgaggua      480

cgauccuacu gcuacgaagc cagcauuuca gacauggcaa gcgacuccag augcccaacc      540

cagggcgaag ccuaccucga caaacaaagc gacacacaau acguaugcaa acgaacacuc      600

guggaccgag gcuggggaaa cggcugcgga cuguucggaa aaggaagccu agugaccugc      660

gcgaaauucg ccugcuccaa gaaaaugaca ggaaaaucaa uccagccuga aaaccucgag      720

uaccgaauaa ugcucagcgu ccauggaagc cagcacuccg gcaugauagu aaacgacaca      780

ggacacgaaa cggacgaaaa cagagcaaaa guagaaauca ccccaaacuc acccagagcc      840

gaagcaacac uuggaggcuu cggcucacuc ggacuagacu gcgagccacg caccggacuc      900

gacuucagcg accucuacua ccuaaccaug aacaacaaac acuggcuugu acacaaagaa      960

ugguuccacg acauaccccu cccuuggcac gcaggagcag acaccggaac accccacugg     1020

aacaacaaag aagcacuggu agaguucaaa gacgcacacg caaaacgaca aaccguaguc     1080

gugcugggca gucaagaagg cgccgugcac accgcccucg cuggagcccu ugaagcagaa     1140

auggacgggg ccaaaggcag acucucaucc ggccaccuca aaugccgacu caaaauggac     1200

aaacuuagac uaaaaggcgu aagcuacagc cugugcacgg cggcauucac auucacgaaa     1260

auaccagccg agacccuaca cggcacuguc accguagaag uacaguacgc aggcacagac     1320

gggcccugca aaguacccgc acaaauggcc guugacaugc aaacccuaac cccagucggc     1380

cgccucauca ccgccaaccc cguaauaaca gaaagcacag aaaacagcaa aaugaugcuc     1440

gaacucgacc caccguucgg agacagcuac aucguaaucg gcgugggcga aaagaaaaua     1500

acgcaucacu ggcaccgauc aggaucaacc aucggcaaag ccuucgaagc aaccguucgc     1560

ggcgcaaaaa gaauggccgu acuaggcgac acggcauggg auuucgguag cguagggggc     1620

gcccuaaacu cccuaggcaa aggcauccac caaaucuucg gcgccgcauu caaaucacua     1680

uucggcggaa ugagcugguu cagccaaauc cucaucggca cccuccucau gugguuagga     1740

cugaaugcca aaaacggaag caucucacuc augugccuag cccugggagg aguauuaauc     1800

uuccuaucua cagcaguauc ggccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10319
<211>  2017
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 1 opt

<400>  10319
gggagaaagc uuaccauggg caaaagauca gccggaucaa ucauguggcu ugcaagccuc       60

gcuguaguaa ucgccugcgc uggagcagca gucacccucc ccucacacuc caccagaaaa      120

cuacaaacac gcucccaaac auggcucgaa ucacgagaau acaccaaaca ccucaucaag      180

gucgaaaacu ggaucuuccg aaaccccgga uucgcacucg uagcaguagc caucgcaugg      240

cuacucggau ccucaaccag ccagaaagua auauaccuag ucaugauccu ccugauagca      300

cccgccuaca gcauccgaug caucggcguc agcaaccgag acuucgucga aggcauguca      360

gggggaaccu gggucgacgu cguccuagag cacggcggcu gcguaacagu cauggcacaa      420

gacaagccca ccgucgacau agaacugguc acaacuaccg uauccaacau ggccgaggua      480

agauccuacu gcuacgaagc cagcauaagc gacauggcca gcgacaguag gugcccuaca      540

caaggcgaag ccuaccucga uaaacaauca gacacccaau augucuguaa acgcacucua      600

guagacagag gauggggcaa cggcugcggc cuauuuggca aaggcagccu agucacaugc      660

gcaaaguuua ccugcagcaa gaaaaugacc ggaaaaucaa uccaaccaga aaaccucgaa      720

uaccgaauca ugcucucagu acacggaucg caacacucag gcaugaucgg cuaugaaacc      780

gacgaagacc gugccaaagu agaaguaacc ccaaacagcc cacgagcuga agcaacccua      840

ggaggcuucg gaucccucgg gcucgacugc gaaccaagaa ccggccuaga cuucuccgac      900

cuguacuacc ucacgaugaa caacaaacac uggcucguac acaaggaaug guuccacgac      960

auaccgcuac ccuggcacgc aggcgcagac acaggcacgc cacacuggaa caacaaagaa     1020

gcccuagugg aguucaaaga cgcacacgca aaacgccaga ccguggucgu ccuagggagc     1080

caagaaggag ccguacacac agcccuggcc ggcgcccuug aagccgaaau ggacggcgca     1140

aaaggccgac ucuucagcgg acaccucaaa ugccgucuca aaauggacaa acuacggcuc     1200

aaaggcguau ccuauucacu cugcaccgcc gccuucacau ucacaaaggu cccugcggaa     1260

acucuacacg gaacagucac agucgaagug caauacgcag gaaccgacgg cccuugcaag     1320

auaccagucc agauggccgu cgacaugcaa acccucacgc cagucggccg acucaucacc     1380

gcaaaccccg uaaucacaga aucaacggaa aacucaaaga ugaugcucga gcucgacccu     1440

cccuucggag acucauacau agucaucggg gucggcgaca agaaaauaac ccaccacugg     1500

cacagaagcg gcuccaccau cggaaaagcc uucgaagcga caguccgagg cgcaaaacgc     1560

auggccguac ucggagacac cgcgugggac uuuggcucag uaggcggcgu guuuaacagc     1620

cucggaaaag gaauccacca aauauucgga gcagcguuca aaucacuguu cggcgggaug     1680

uccugguucu cccaaauccu caucggaaca cuucucguau ggcuaggccu aaacaccaaa     1740

aacggaucaa ucucacuaac augccucgcc cucggaggag ucaugauauu ccugucgacu     1800

gcggucucgg ccugaggacu aguuauaaga cugacuagcc cgaugggccu cccaacgggc     1860

ccuccucccc uccuugcacc gagauuaaua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     1980

ccccccccca aaggcucuuu ucagagccac cagaauu                              2017


<210>  10320
<211>  2029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 1 opt

<400>  10320
gggagaaagc uuaccauggg aaagcgaucc gcaggaucca uaauguggcu cgccucccua       60

gccgucguaa uagcgugcgc cggcgcagcc guaacccuac ccucccacag cacccgcaag      120

cuacaaacac gcucacaaac cuggcuugaa ucacgcgagu acacuaaaca ccuaauacgc      180

guagaaaacu ggaucuuccg uaaccccggg uucgcacucg ccgcagccgc caucgccugg      240

cuucuaggaa gcuccacauc acaaaaaguc aucuaccuag ucaugauccu ccucauugcc      300

ccagccuaca guaucagaug caucgguguc uccaaccggg acuucgucga agggaugucc      360

ggaggaacau ggguagacgu cguccucgaa cacggcggcu gcguaaccgu aauggcgcag      420

gacaaaccga cugucgacau agagcuagua accacuaccg ucuccaacau ggcagaagua      480

cgauccuacu gcuacgaagc cagcauuagc gacauggcau ccgacagccg cugccccacc      540

caaggcgaag ccuaccucga caaacaauca gacacccaau acgucugcaa acgcacacuc      600

gucgaccgug gcuggggcaa cggcugcgga cucuucggaa agggcucccu aguuaccugc      660

gcaaaguucg ccuguuccaa gaaaaugacc ggcaaaucga uacaaccaga aaaccucgaa      720

uaucgaauaa ugcucuccgu ccacggcagc caacacagcg gcaugauagu aaacgacacc      780

ggacacgaaa cagacgaaaa ccgcgcuaaa gucgaaauaa cacccaacuc accccgggcc      840

gaagccaccc ucggcggcuu cggaucacuc ggccucgacu gcgagcccag aaccggccua      900

gacuucucgg accucuacua ucuaacaaug aacaacaaac acuggcucgu ccacaaagaa      960

ugguuccacg acaucccacu ccccuggcac gccggagccg acaccggaac accucacugg     1020

aacaacaaag aagcccucgu cgaguucaaa gacgcccacg ccaaacgcca gacaguggua     1080

guacuaggca gccaagaagg agccguccac acggcccuag caggagcccu ggaagccgaa     1140

auggacggag caaaaggcag acucucuagc ggccaccuca agugccgccu aaaaauggac     1200

aaacuacgac uaaaaggcgu uuccuacagc cugugcaccg ccgccuucac guucacuaaa     1260

auacccgccg aaacccucca cggcaccgua acagucgaag uccaauacgc aggaaccgac     1320

ggaccgugca aaguaccugc acaaauggcc guagacaugc agacccugac cccaguaggc     1380

agacuaauca cagccaaccc cguaauaacg gaaucaaccg aaaacucaaa aaugaugcuu     1440

gaacuagacc cgcccuucgg ggacaguuac aucguaaucg gcgucggaga aaagaaaaua     1500

acccaccacu ggcacagauc cggaucaacc aucggaaagg ccuucgaagc aaccguccgg     1560

ggagccaaac gcauggccgu ccuaggagac acagccuggg acuucggcag ugucggcggc     1620

gcccucaaca gucugggaaa aggcauacac caaaucuucg gcgcagcauu caagagccua     1680

uucgggggaa ugagcugguu cucccaaaua cuaauaggaa cccuccuaau guggcuggga     1740

cucaacacca aaaacggcag caucucacuc augugccucg cccucggcgg aguacugaua     1800

uuccucucua cagcagucuc cgccugagga cuaguuauaa gacugacuag cccgaugggc     1860

cucccaacgg gcccuccucc ccuccuugca ccgagauuaa uaaaaaaaaa aaaaaaaaaa     1920

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     1980

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2029


<210>  10321
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 wild 
       type

<400>  10321
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga gguuguguca      720

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu     1260

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1860

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1920

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu uuuggagcag     1980

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu ggaacguugc     2040

ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     2100

ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10322
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 wild 
       type

<400>  10322
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca cacgugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auguugucuu ggaacaugga gguuguguca      720

cuguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gccuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaaaguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg gguuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggc     1260

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu acugcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1860

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1920

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauc uuuggagcag     1980

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu ggaacguugc     2040

ugaugugguu gggucugaac gcaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     2100

ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10323
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 wild type

<400>  10323
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcuau cacuagacgc gggagugcau acuacaugua cuuggauagg agcgaugccg      180

ggaaggccau uucguuugcu accacauugg gagugaacaa gugccacgua cagaucaugg      240

accucgggca caugugugac gccaccauga guuaugagug cccuaugcug gaugagggag      300

uggaaccaga ugaugucgau ugcuggugca acacgacauc aacuuggguu guguacggaa      360

ccugucauca caaaaaaggu gaggcacggc gaucuagaag agccgugacg cucccuucuc      420

acucuacaag gaaguugcaa acgcggucgc agaccugguu agaaucaaga gaauacacga      480

agcacuugau caagguugaa aacuggauau ucaggaaccc cggguuugcg cuaguggccg      540

uugccauugc cuggcuuuug ggaagcucga cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacaguauca ggugcauugg agucagcaau agagacuucg      660

uggagggcau gucagguggg accuggguug auguugucuu ggaacaugga ggcugcguua      720

ccgugauggc acaggacaag ccaacagucg acauagaguu ggucacgacg acgguuagua      780

acauggccga gguaagaucc uauugcuacg aggcaucgau aucggacaug gcuucggaca      840

gucguugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac auuaguggac agagguuggg gaaacgguug uggacuuuuu ggcaaaggga      960

gcuuggugac augugccaag uuuacguguu cuaagaagau gaccgggaag agcauucaac     1020

cggaaaaucu ggaguaucgg auaaugcuau cagugcaugg cucccagcau agcgggauga     1080

uuggauauga aacugacgaa gauagagcga aagucgaggu uacgccuaau ucaccaagag     1140

cggaagcaac cuugggaggc uuuggaaguu uaggacuuga cugugaacca aggacaggcc     1200

uugacuuuuc agaucuguau uaccugacca ugaacaauaa gcauugguug gugcacaaag     1260

agugguuuca ugacauccca uugccuuggc augcuggggc agacaccgga acuccacacu     1320

ggaacaacaa agaggcauug guagaauuua aggaugccca cgccaagagg caaaccgucg     1380

ucguucuggg gagccaggaa ggagccguuc acacggcucu cgcuggagcu cuagaggcug     1440

agauggaugg ugcaaaggga aggcuguucu cuggccauuu gaaaugccgc cuaaaaaugg     1500

acaagcucag auugaagggc gugucauauu ccuugugcac ugcggcauuc acauucacca     1560

aggucccagc ugaaacacug cauggaacag ucacagugga ggugcaguau gcagggacag     1620

auggacccug caagauccca guccagaugg cgguggacau gcagacccug accccaguug     1680

gaaggcugau aaccgccaac cccgugauua cugaaagcac ugagaacuca aagaugaugu     1740

uggagcuuga cccaccauuu ggggauucuu acauugucau aggaguuggg gacaagaaaa     1800

ucacccacca cuggcauagg agugguagca ccaucggaaa ggcauuugag gccacuguga     1860

gaggcgccaa gagaauggca guccuggggg auacagccug ggacuucgga ucagucgggg     1920

guguguucaa cucacugggu aagggcauuc accagauuuu uggagcagcc uucaaaucac     1980

uguuuggagg aauguccugg uucucacaga uccucauagg cacgcugcua gugugguuag     2040

guuugaacac aaagaaugga ucuaucuccc ucacaugcuu ggcccugggg ggagugauga     2100

ucuuccucuc cacggcuguu ucugcuugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10324
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 wild type

<400>  10324
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auguugucuu ggaacaugga gguuguguca      720

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gccuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu     1260

ugguccacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1860

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1920

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu uuuggagcag     1980

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauccucauu ggaacguugc     2040

ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     2100

ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10325
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 wild 
       type

<400>  10325
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10326
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 wild 
       type

<400>  10326
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac acgugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac uguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaaucu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auucucauug gaacguugcu gaugugguug ggucugaacg     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10327
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 wild type

<400>  10327
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau uggauaugaa acugacgaag     1080

auagagcgaa agucgagguu acgccuaauu caccaagagc ggaagcaacc uugggaggcu     1140

uuggaaguuu aggacuugac ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu     1200

accugaccau gaacaauaag cauugguugg ugcacaaaga gugguuucau gacaucccau     1260

ugccuuggca ugcuggggca gacaccggaa cuccacacug gaacaacaaa gaggcauugg     1320

uagaauuuaa ggaugcccac gccaagaggc aaaccgucgu cguucugggg agccaggaag     1380

gagccguuca cacggcucuc gcuggagcuc uagaggcuga gauggauggu gcaaagggaa     1440

ggcuguucuc uggccauuug aaaugccgcc uaaaaaugga caagcucaga uugaagggcg     1500

ugucauauuc cuugugcacu gcggcauuca cauucaccaa ggucccagcu gaaacacugc     1560

auggaacagu cacaguggag gugcaguaug cagggacaga uggacccugc aagaucccag     1620

uccagauggc gguggacaug cagacccuga ccccaguugg aaggcugaua accgccaacc     1680

ccgugauuac ugaaagcacu gagaacucaa agaugauguu ggagcuugac ccaccauuug     1740

gggauucuua cauugucaua ggaguugggg acaagaaaau cacccaccac uggcauagga     1800

gugguagcac caucggaaag gcauuugagg ccacugugag aggcgccaag agaauggcag     1860

uccuggggga uacagccugg gacuucggau cagucggggg uguguucaac ucacugggua     1920

agggcauuca ccagauuuuu ggagcagccu ucaaaucacu guuuggagga auguccuggu     1980

ucucacagau ccucauaggc acgcugcuag ugugguuagg uuugaacaca aagaauggau     2040

cuaucucccu cacaugcuug gcccuggggg gagugaugau cuuccucucc acggcuguuu     2100

cugcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10328
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 wild type

<400>  10328
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc accauuggaa aagcauuuga agccacugug agaggugcca     1860

agagaauggc agucuuggga gacacagccu gggacuuugg aucaguugga ggcgcucuca     1920

acucauuggg caagggcauc caucaaauuu uuggagcagc uuucaaauca uuguuuggag     1980

gaauguccug guucucacaa auccucauug gaacguugcu gaugugguug ggucugaaca     2040

caaagaaugg aucuauuucc cuuaugugcu uggccuuagg gggaguguug aucuucuuau     2100

ccacagccgu cucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10329
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 wild 
       type

<400>  10329
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu ugugucaccg      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggaguc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1260

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     1860

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     1920

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu ggagcagcuu     1980

ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga acguugcuga     2040

ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2100

gaguguugau cuucuuaucc acagccgucu cugcuugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10330
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 wild 
       type

<400>  10330
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacac gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu ugugucacug      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggagcc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa aguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggggu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacuggcugg     1260

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacu gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     1860

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     1920

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaaucuuu ggagcagcuu     1980

ucaaaucauu guuuggagga auguccuggu ucucacaaau ucucauugga acguugcuga     2040

ugugguuggg ucugaacgca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2100

gaguguugau cuucuuaucc acagccgucu cugcuugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10331
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 wild type

<400>  10331
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccaucac uagacgcggg agugcauacu acauguacuu ggauaggagc gaugccggga      180

aggccauuuc guuugcuacc acauugggag ugaacaagug ccacguacag aucauggacc      240

ucgggcacau gugugacgcc accaugaguu augagugccc uaugcuggau gagggagugg      300

aaccagauga ugucgauugc uggugcaaca cgacaucaac uuggguugug uacggaaccu      360

gucaucacaa aaaaggugag gcacggcgau cuagaagagc cgugacgcuc ccuucucacu      420

cuacaaggaa guugcaaacg cggucgcaga ccugguuaga aucaagagaa uacacgaagc      480

acuugaucaa gguugaaaac uggauauuca ggaaccccgg guuugcgcua guggccguug      540

ccauugccug gcuuuuggga agcucgacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac aguaucaggu gcauuggagu cagcaauaga gacuucgugg      660

agggcauguc aggugggacc uggguugaug uugucuugga acauggaggc ugcguuaccg      720

ugauggcaca ggacaagcca acagucgaca uagaguuggu cacgacgacg guuaguaaca      780

uggccgaggu aagauccuau ugcuacgagg caucgauauc ggacauggcu ucggacaguc      840

guugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacauu aguggacaga gguuggggaa acgguugugg acuuuuuggc aaagggagcu      960

uggugacaug ugccaaguuu acguguucua agaagaugac cgggaagagc auucaaccgg     1020

aaaaucugga guaucggaua augcuaucag ugcauggcuc ccagcauagc gggaugauug     1080

gauaugaaac ugacgaagau agagcgaaag ucgagguuac gccuaauuca ccaagagcgg     1140

aagcaaccuu gggaggcuuu ggaaguuuag gacuugacug ugaaccaagg acaggccuug     1200

acuuuucaga ucuguauuac cugaccauga acaauaagca uugguuggug cacaaagagu     1260

gguuucauga caucccauug ccuuggcaug cuggggcaga caccggaacu ccacacugga     1320

acaacaaaga ggcauuggua gaauuuaagg augcccacgc caagaggcaa accgucgucg     1380

uucuggggag ccaggaagga gccguucaca cggcucucgc uggagcucua gaggcugaga     1440

uggauggugc aaagggaagg cuguucucug gccauuugaa augccgccua aaaauggaca     1500

agcucagauu gaagggcgug ucauauuccu ugugcacugc ggcauucaca uucaccaagg     1560

ucccagcuga aacacugcau ggaacaguca caguggaggu gcaguaugca gggacagaug     1620

gacccugcaa gaucccaguc cagauggcgg uggacaugca gacccugacc ccaguuggaa     1680

ggcugauaac cgccaacccc gugauuacug aaagcacuga gaacucaaag augauguugg     1740

agcuugaccc accauuuggg gauucuuaca uugucauagg aguuggggac aagaaaauca     1800

cccaccacug gcauaggagu gguagcacca ucggaaaggc auuugaggcc acugugagag     1860

gcgccaagag aauggcaguc cugggggaua cagccuggga cuucggauca gucgggggug     1920

uguucaacuc acuggguaag ggcauucacc agauuuuugg agcagccuuc aaaucacugu     1980

uuggaggaau guccugguuc ucacagaucc ucauaggcac gcugcuagug ugguuagguu     2040

ugaacacaaa gaauggaucu aucucccuca caugcuuggc ccugggggga gugaugaucu     2100

uccucuccac ggcuguuucu gcuugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10332
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 wild type

<400>  10332
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu ugugucaccg      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggagcc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1260

uccacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac cauuggaaaa gcauuugaag     1860

ccacugugag aggugccaag agaauggcag ucuugggaga cacagccugg gacuuuggau     1920

caguuggagg cgcucucaac ucauugggca agggcaucca ucaaauuuuu ggagcagcuu     1980

ucaaaucauu guuuggagga auguccuggu ucucacaaau ccucauugga acguugcuga     2040

ugugguuggg ucugaacaca aagaauggau cuauuucccu uaugugcuug gccuuagggg     2100

gaguguugau cuucuuaucc acagccgucu cugcuugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10333
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 wild 
       type

<400>  10333
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga gguuguguca      720

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu     1260

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag cacgcugggc aaggccuuuu     1860

caacaacuuu gaagggagcu caaagacugg cagcguuggg cgacacagcc ugggacuuug     1920

gcucuauugg aggggucuuc aacuccauag gaagagccgu ucaccaagug uuugguggug     1980

ccuucagaac acucuuuggg ggaaugucuu ggaucacaca agggcuaaug ggugcccuac     2040

ugcucuggau gggcgucaac gcacgagacc gaucaauugc uuuggccuuc uuagccacag     2100

gaggugugcu cguguucuua gcgaccaaug ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10334
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 wild 
       type

<400>  10334
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca cacgugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auguugucuu ggaacaugga gguuguguca      720

cuguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gccuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaaaguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg gguuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggc     1260

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu acugcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag cacgcugggc aaggccuuuu     1860

caacaacuuu gaagggagcu caaagacugg cagcguuggg cgacacagcc ugggacuuug     1920

gcucuauugg aggggucuuc aacuccauag gaagagccgu ucaccaagug uuugguggug     1980

ccuucagaac acucuuuggg ggaaugucuu ggaucacaca agggcuaaug ggugcccuac     2040

ugcucuggau gggcgucaac gcacgagacc gaucaauugc uuuggccuuc uuagccacag     2100

gaggugugcu cguguucuua gcgaccaaug ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10335
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 wild type

<400>  10335
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcuau cacuagacgc gggagugcau acuacaugua cuuggauagg agcgaugccg      180

ggaaggccau uucguuugcu accacauugg gagugaacaa gugccacgua cagaucaugg      240

accucgggca caugugugac gccaccauga guuaugagug cccuaugcug gaugagggag      300

uggaaccaga ugaugucgau ugcuggugca acacgacauc aacuuggguu guguacggaa      360

ccugucauca caaaaaaggu gaggcacggc gaucuagaag agccgugacg cucccuucuc      420

acucuacaag gaaguugcaa acgcggucgc agaccugguu agaaucaaga gaauacacga      480

agcacuugau caagguugaa aacuggauau ucaggaaccc cggguuugcg cuaguggccg      540

uugccauugc cuggcuuuug ggaagcucga cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacaguauca ggugcauugg agucagcaau agagacuucg      660

uggagggcau gucagguggg accuggguug auguugucuu ggaacaugga ggcugcguua      720

ccgugauggc acaggacaag ccaacagucg acauagaguu ggucacgacg acgguuagua      780

acauggccga gguaagaucc uauugcuacg aggcaucgau aucggacaug gcuucggaca      840

gucguugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac auuaguggac agagguuggg gaaacgguug uggacuuuuu ggcaaaggga      960

gcuuggugac augugccaag uuuacguguu cuaagaagau gaccgggaag agcauucaac     1020

cggaaaaucu ggaguaucgg auaaugcuau cagugcaugg cucccagcau agcgggauga     1080

uuggauauga aacugacgaa gauagagcga aagucgaggu uacgccuaau ucaccaagag     1140

cggaagcaac cuugggaggc uuuggaaguu uaggacuuga cugugaacca aggacaggcc     1200

uugacuuuuc agaucuguau uaccugacca ugaacaauaa gcauugguug gugcacaaag     1260

agugguuuca ugacauccca uugccuuggc augcuggggc agacaccgga acuccacacu     1320

ggaacaacaa agaggcauug guagaauuua aggaugccca cgccaagagg caaaccgucg     1380

ucguucuggg gagccaggaa ggagccguuc acacggcucu cgcuggagcu cuagaggcug     1440

agauggaugg ugcaaaggga aggcuguucu cuggccauuu gaaaugccgc cuaaaaaugg     1500

acaagcucag auugaagggc gugucauauu ccuugugcac ugcggcauuc acauucacca     1560

aggucccagc ugaaacacug cauggaacag ucacagugga ggugcaguau gcagggacag     1620

auggacccug caagauccca guccagaugg cgguggacau gcagacccug accccaguug     1680

gaaggcugau aaccgccaac cccgugauua cugaaagcac ugagaacuca aagaugaugu     1740

uggagcuuga cccaccauuu ggggauucuu acauugucau aggaguuggg gacaagaaaa     1800

ucacccacca cuggcauagg aguggcagca cgcugggcaa ggccuuuuca acaacuuuga     1860

agggagcuca aagacuggca gcguugggcg acacagccug ggacuuuggc ucuauuggag     1920

gggucuucaa cuccauagga agagccguuc accaaguguu ugguggugcc uucagaacac     1980

ucuuuggggg aaugucuugg aucacacaag ggcuaauggg ugcccuacug cucuggaugg     2040

gcgucaacgc acgagaccga ucaauugcuu uggccuucuu agccacagga ggugugcucg     2100

uguucuuagc gaccaaugug cacgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10336
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 wild type

<400>  10336
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugu cacuagacgu gggagugcau acuauaugua cuuggacaga aacgaugcug      180

gggaggccau aucuuuucca accacauugg ggaugaauaa guguuauaua cagaucaugg      240

aucuuggaca caugugugau gccaccauga gcuaugaaug cccuaugcug gaugaggggg      300

uggaaccaga ugacgucgau uguuggugca acacgacguc aacuuggguu guguacggaa      360

ccugccauca caaaaaaggu gaagcacgga gaucuagaag agcugugacg cuccccuccc      420

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      480

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      540

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      600

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      660

uggaagguau gucagguggg acuuggguug auguugucuu ggaacaugga gguuguguca      720

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      780

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      840

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      900

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      960

gccuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc     1020

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga     1080

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca     1140

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac     1200

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu     1260

ugguccacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1320

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1380

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1440

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1500

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1560

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1620

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1680

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1740

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1800

gggagaagaa gaucacccac cacuggcaca ggaguggcag cacgcugggc aaggccuuuu     1860

caacaacuuu gaagggagcu caaagacugg cagcguuggg cgacacagcc ugggacuuug     1920

gcucuauugg aggggucuuc aacuccauag gaagagccgu ucaccaagug uuugguggug     1980

ccuucagaac acucuuuggg ggaaugucuu ggaucacaca agggcuaaug ggugcccuac     2040

ugcucuggau gggcgucaac gcacgagacc gaucaauugc uuuggccuuc uuagccacag     2100

gaggugugcu cguguucuua gcgaccaaug ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10337
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 wild 
       type

<400>  10337
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uauugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc acgcugggca aggccuuuuc aacaacuuug aagggagcuc     1860

aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga ggggucuuca     1920

acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca cucuuugggg     1980

gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg     2040

cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc guguucuuag     2100

cgaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10338
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 wild 
       type

<400>  10338
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac acgugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac uguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aaaguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg guuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacuggcu gguucacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua cugcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc acgcugggca aggccuuuuc aacaacuuug aagggagcuc     1860

aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga ggggucuuca     1920

acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca cucuuugggg     1980

gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg     2040

cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc guguucuuag     2100

cgaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10339
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 wild type

<400>  10339
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcauc acuagacgcg      120

ggagugcaua cuacauguac uuggauagga gcgaugccgg gaaggccauu ucguuugcua      180

ccacauuggg agugaacaag ugccacguac agaucaugga ccucgggcac augugugacg      240

ccaccaugag uuaugagugc ccuaugcugg augagggagu ggaaccagau gaugucgauu      300

gcuggugcaa cacgacauca acuuggguug uguacggaac cugucaucac aaaaaaggug      360

aggcacggcg aucuagaaga gccgugacgc ucccuucuca cucuacaagg aaguugcaaa      420

cgcggucgca gaccugguua gaaucaagag aauacacgaa gcacuugauc aagguugaaa      480

acuggauauu caggaacccc ggguuugcgc uaguggccgu ugccauugcc uggcuuuugg      540

gaagcucgac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acaguaucag gugcauugga gucagcaaua gagacuucgu ggagggcaug ucagguggga      660

ccuggguuga uguugucuug gaacauggag gcugcguuac cgugauggca caggacaagc      720

caacagucga cauagaguug gucacgacga cgguuaguaa cauggccgag guaagauccu      780

auugcuacga ggcaucgaua ucggacaugg cuucggacag ucguugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaaca uuaguggaca      900

gagguugggg aaacgguugu ggacuuuuug gcaaagggag cuuggugaca ugugccaagu      960

uuacguguuc uaagaagaug accgggaaga gcauucaacc ggaaaaucug gaguaucgga     1020

uaaugcuauc agugcauggc ucccagcaua gcgggaugau uggauaugaa acugacgaag     1080

auagagcgaa agucgagguu acgccuaauu caccaagagc ggaagcaacc uugggaggcu     1140

uuggaaguuu aggacuugac ugugaaccaa ggacaggccu ugacuuuuca gaucuguauu     1200

accugaccau gaacaauaag cauugguugg ugcacaaaga gugguuucau gacaucccau     1260

ugccuuggca ugcuggggca gacaccggaa cuccacacug gaacaacaaa gaggcauugg     1320

uagaauuuaa ggaugcccac gccaagaggc aaaccgucgu cguucugggg agccaggaag     1380

gagccguuca cacggcucuc gcuggagcuc uagaggcuga gauggauggu gcaaagggaa     1440

ggcuguucuc uggccauuug aaaugccgcc uaaaaaugga caagcucaga uugaagggcg     1500

ugucauauuc cuugugcacu gcggcauuca cauucaccaa ggucccagcu gaaacacugc     1560

auggaacagu cacaguggag gugcaguaug cagggacaga uggacccugc aagaucccag     1620

uccagauggc gguggacaug cagacccuga ccccaguugg aaggcugaua accgccaacc     1680

ccgugauuac ugaaagcacu gagaacucaa agaugauguu ggagcuugac ccaccauuug     1740

gggauucuua cauugucaua ggaguugggg acaagaaaau cacccaccac uggcauagga     1800

guggcagcac gcugggcaag gccuuuucaa caacuuugaa gggagcucaa agacuggcag     1860

cguugggcga cacagccugg gacuuuggcu cuauuggagg ggucuucaac uccauaggaa     1920

gagccguuca ccaaguguuu gguggugccu ucagaacacu cuuuggggga augucuugga     1980

ucacacaagg gcuaaugggu gcccuacugc ucuggauggg cgucaacgca cgagaccgau     2040

caauugcuuu ggccuucuua gccacaggag gugugcucgu guucuuagcg accaaugugc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10340
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 wild type

<400>  10340
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acuagacgug      120

ggagugcaua cuauauguac uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa      180

ccacauuggg gaugaauaag uguuauauac agaucaugga ucuuggacac augugugaug      240

ccaccaugag cuaugaaugc ccuaugcugg augagggggu ggaaccagau gacgucgauu      300

guuggugcaa cacgacguca acuuggguug uguacggaac cugccaucac aaaaaaggug      360

aagcacggag aucuagaaga gcugugacgc uccccuccca uuccacuagg aagcugcaaa      420

cgcggucgca aaccugguug gaaucaagag aauacacaaa gcacuugauu agagucgaaa      480

auuggauauu caggaacccu ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg      540

gaagcucaac gagccaaaaa gucauauacu uggucaugau acugcugauu gccccggcau      600

acagcaucag gugcauagga gucagcaaua gggacuuugu ggaagguaug ucagguggga      660

cuuggguuga uguugucuug gaacauggag guugugucac cguaauggca caggacaaac      720

cgacugucga cauagagcug guuacaacaa cagucagcaa cauggcggag guaagauccu      780

acugcuauga ggcaucaaua ucagacaugg cuucggacag ccgcugccca acacaaggug      840

aagccuaccu ugacaagcaa ucagacacuc aauaugucug caaaagaacg uuaguggaca      900

gaggcugggg aaauggaugu ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu      960

uugcaugcuc caagaaaaug accgggaaga gcauccagcc agagaaucug gaguaccgga     1020

uaaugcuguc aguucauggc ucccagcaca gugggaugau cguuaaugac acaggacaug     1080

aaacugauga gaauagagcg aagguugaga uaacgcccaa uucaccaaga gccgaagcca     1140

cccugggggg uuuuggaagc cuaggacuug auugugaacc gaggacaggc cuugacuuuu     1200

cagauuugua uuacuugacu augaauaaca agcacugguu gguccacaag gagugguucc     1260

acgacauucc auuaccuugg cacgcugggg cagacaccgg aacuccacac uggaacaaca     1320

aagaagcacu gguagaguuc aaggacgcac augccaaaag gcaaacuguc gugguucuag     1380

ggagucaaga aggagcaguu cacacggccc uugcuggagc ucuggaggcu gagauggaug     1440

gugcaaaggg aaggcugucc ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua     1500

gauugaaggg cgugucauac uccuugugua ccgcagcguu cacauucacc aagaucccgg     1560

cugaaacacu gcacgggaca gucacagugg agguacagua cgcagggaca gauggaccuu     1620

gcaagguucc agcucagaug gcgguggaca ugcaaacucu gaccccaguu gggagguuga     1680

uaaccgcuaa ccccguaauc acugaaagca cugagaacuc uaagaugaug cuggaacuug     1740

auccaccauu uggggacucu uacauuguca uaggagucgg ggagaagaag aucacccacc     1800

acuggcacag gaguggcagc acgcugggca aggccuuuuc aacaacuuug aagggagcuc     1860

aaagacuggc agcguugggc gacacagccu gggacuuugg cucuauugga ggggucuuca     1920

acuccauagg aagagccguu caccaagugu uugguggugc cuucagaaca cucuuugggg     1980

gaaugucuug gaucacacaa gggcuaaugg gugcccuacu gcucuggaug ggcgucaacg     2040

cacgagaccg aucaauugcu uuggccuucu uagccacagg aggugugcuc guguucuuag     2100

cgaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10341
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 wild 
       type

<400>  10341
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaua uugucuugga acauggaggu ugugucaccg      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggaguc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1260

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac gcugggcaag gccuuuucaa     1860

caacuuugaa gggagcucaa agacuggcag cguugggcga cacagccugg gacuuuggcu     1920

cuauuggagg ggucuucaac uccauaggaa gagccguuca ccaaguguuu gguggugccu     1980

ucagaacacu cuuuggggga augucuugga ucacacaagg gcuaaugggu gcccuacugc     2040

ucuggauggg cgucaacgca cgagaccgau caauugcuuu ggccuucuua gccacaggag     2100

gugugcucgu guucuuagcg accaaugugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10342
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 wild 
       type

<400>  10342
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacac gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu ugugucacug      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggagcc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa aguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggggu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacuggcugg     1260

uucacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacu gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac gcugggcaag gccuuuucaa     1860

caacuuugaa gggagcucaa agacuggcag cguugggcga cacagccugg gacuuuggcu     1920

cuauuggagg ggucuucaac uccauaggaa gagccguuca ccaaguguuu gguggugccu     1980

ucagaacacu cuuuggggga augucuugga ucacacaagg gcuaaugggu gcccuacugc     2040

ucuggauggg cgucaacgca cgagaccgau caauugcuuu ggccuucuua gccacaggag     2100

gugugcucgu guucuuagcg accaaugugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10343
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 wild type

<400>  10343
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccaucac uagacgcggg agugcauacu acauguacuu ggauaggagc gaugccggga      180

aggccauuuc guuugcuacc acauugggag ugaacaagug ccacguacag aucauggacc      240

ucgggcacau gugugacgcc accaugaguu augagugccc uaugcuggau gagggagugg      300

aaccagauga ugucgauugc uggugcaaca cgacaucaac uuggguugug uacggaaccu      360

gucaucacaa aaaaggugag gcacggcgau cuagaagagc cgugacgcuc ccuucucacu      420

cuacaaggaa guugcaaacg cggucgcaga ccugguuaga aucaagagaa uacacgaagc      480

acuugaucaa gguugaaaac uggauauuca ggaaccccgg guuugcgcua guggccguug      540

ccauugccug gcuuuuggga agcucgacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac aguaucaggu gcauuggagu cagcaauaga gacuucgugg      660

agggcauguc aggugggacc uggguugaug uugucuugga acauggaggc ugcguuaccg      720

ugauggcaca ggacaagcca acagucgaca uagaguuggu cacgacgacg guuaguaaca      780

uggccgaggu aagauccuau ugcuacgagg caucgauauc ggacauggcu ucggacaguc      840

guugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacauu aguggacaga gguuggggaa acgguugugg acuuuuuggc aaagggagcu      960

uggugacaug ugccaaguuu acguguucua agaagaugac cgggaagagc auucaaccgg     1020

aaaaucugga guaucggaua augcuaucag ugcauggcuc ccagcauagc gggaugauug     1080

gauaugaaac ugacgaagau agagcgaaag ucgagguuac gccuaauuca ccaagagcgg     1140

aagcaaccuu gggaggcuuu ggaaguuuag gacuugacug ugaaccaagg acaggccuug     1200

acuuuucaga ucuguauuac cugaccauga acaauaagca uugguuggug cacaaagagu     1260

gguuucauga caucccauug ccuuggcaug cuggggcaga caccggaacu ccacacugga     1320

acaacaaaga ggcauuggua gaauuuaagg augcccacgc caagaggcaa accgucgucg     1380

uucuggggag ccaggaagga gccguucaca cggcucucgc uggagcucua gaggcugaga     1440

uggauggugc aaagggaagg cuguucucug gccauuugaa augccgccua aaaauggaca     1500

agcucagauu gaagggcgug ucauauuccu ugugcacugc ggcauucaca uucaccaagg     1560

ucccagcuga aacacugcau ggaacaguca caguggaggu gcaguaugca gggacagaug     1620

gacccugcaa gaucccaguc cagauggcgg uggacaugca gacccugacc ccaguuggaa     1680

ggcugauaac cgccaacccc gugauuacug aaagcacuga gaacucaaag augauguugg     1740

agcuugaccc accauuuggg gauucuuaca uugucauagg aguuggggac aagaaaauca     1800

cccaccacug gcauaggagu ggcagcacgc ugggcaaggc cuuuucaaca acuuugaagg     1860

gagcucaaag acuggcagcg uugggcgaca cagccuggga cuuuggcucu auuggagggg     1920

ucuucaacuc cauaggaaga gccguucacc aaguguuugg uggugccuuc agaacacucu     1980

uugggggaau gucuuggauc acacaagggc uaaugggugc ccuacugcuc uggaugggcg     2040

ucaacgcacg agaccgauca auugcuuugg ccuucuuagc cacaggaggu gugcucgugu     2100

ucuuagcgac caaugugcac gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10344
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 wild type

<400>  10344
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgucac uagacguggg agugcauacu auauguacuu ggacagaaac gaugcugggg      180

aggccauauc uuuuccaacc acauugggga ugaauaagug uuauauacag aucauggauc      240

uuggacacau gugugaugcc accaugagcu augaaugccc uaugcuggau gagggggugg      300

aaccagauga cgucgauugu uggugcaaca cgacgucaac uuggguugug uacggaaccu      360

gccaucacaa aaaaggugaa gcacggagau cuagaagagc ugugacgcuc cccucccauu      420

ccacuaggaa gcugcaaacg cggucgcaaa ccugguugga aucaagagaa uacacaaagc      480

acuugauuag agucgaaaau uggauauuca ggaacccugg cuucgcguua gcagcagcug      540

ccaucgcuug gcuuuuggga agcucaacga gccaaaaagu cauauacuug gucaugauac      600

ugcugauugc cccggcauac agcaucaggu gcauaggagu cagcaauagg gacuuugugg      660

aagguauguc aggugggacu uggguugaug uugucuugga acauggaggu ugugucaccg      720

uaauggcaca ggacaaaccg acugucgaca uagagcuggu uacaacaaca gucagcaaca      780

uggcggaggu aagauccuac ugcuaugagg caucaauauc agacauggcu ucggacagcc      840

gcugcccaac acaaggugaa gccuaccuug acaagcaauc agacacucaa uaugucugca      900

aaagaacguu aguggacaga ggcuggggaa auggaugugg acuuuuuggc aaagggagcc      960

uggugacaug cgcuaaguuu gcaugcucca agaaaaugac cgggaagagc auccagccag     1020

agaaucugga guaccggaua augcugucag uucauggcuc ccagcacagu gggaugaucg     1080

uuaaugacac aggacaugaa acugaugaga auagagcgaa gguugagaua acgcccaauu     1140

caccaagagc cgaagccacc cugggggguu uuggaagccu aggacuugau ugugaaccga     1200

ggacaggccu ugacuuuuca gauuuguauu acuugacuau gaauaacaag cacugguugg     1260

uccacaagga gugguuccac gacauuccau uaccuuggca cgcuggggca gacaccggaa     1320

cuccacacug gaacaacaaa gaagcacugg uagaguucaa ggacgcacau gccaaaaggc     1380

aaacugucgu gguucuaggg agucaagaag gagcaguuca cacggcccuu gcuggagcuc     1440

uggaggcuga gauggauggu gcaaagggaa ggcuguccuc uggccacuug aaaugucgcc     1500

ugaaaaugga uaaacuuaga uugaagggcg ugucauacuc cuuguguacc gcagcguuca     1560

cauucaccaa gaucccggcu gaaacacugc acgggacagu cacaguggag guacaguacg     1620

cagggacaga uggaccuugc aagguuccag cucagauggc gguggacaug caaacucuga     1680

ccccaguugg gagguugaua accgcuaacc ccguaaucac ugaaagcacu gagaacucua     1740

agaugaugcu ggaacuugau ccaccauuug gggacucuua cauugucaua ggagucgggg     1800

agaagaagau cacccaccac uggcacagga guggcagcac gcugggcaag gccuuuucaa     1860

caacuuugaa gggagcucaa agacuggcag cguugggcga cacagccugg gacuuuggcu     1920

cuauuggagg ggucuucaac uccauaggaa gagccguuca ccaaguguuu gguggugccu     1980

ucagaacacu cuuuggggga augucuugga ucacacaagg gcuaaugggu gcccuacugc     2040

ucuggauggg cgucaacgca cgagaccgau caauugcuuu ggccuucuua gccacaggag     2100

gugugcucgu guucuuagcg accaaugugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10345
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 wild type

<400>  10345
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcugugacg cuccccuccc      120

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      180

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      240

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      300

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      360

uggaagguau gucagguggg acuuggguug auauugucuu ggaacaugga gguuguguca      420

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      480

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      540

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      600

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      660

gucuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc      720

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga      780

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca      840

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac      900

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu      960

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1020

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1080

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1140

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1200

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1260

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1320

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1380

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1440

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1500

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1560

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1620

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu uuuggagcag     1680

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu ggaacguugc     1740

ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     1800

ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10346
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 wild type

<400>  10346
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacga       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcugugacg cuccccuccc      120

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      180

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      240

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      300

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      360

uggaagguau gucagguggg acuuggguug auguugucuu ggaacaugga gguuguguca      420

cuguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      480

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      540

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      600

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      660

gccuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc      720

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga      780

ucguuaauga cacaggacau gaaacugaug agaauagagc gaaaguugag auaacgccca      840

auucaccaag agccgaagcc acccuggggg gguuuggaag ccuaggacuu gauugugaac      900

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggc      960

ugguucacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1020

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1080

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1140

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1200

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu acugcagcgu     1260

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1320

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1380

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1440

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1500

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1560

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1620

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauc uuuggagcag     1680

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauucucauu ggaacguugc     1740

ugaugugguu gggucugaac gcaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     1800

ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10347
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 wild type

<400>  10347
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggaau cauuggccuc cugcugacua cagccauggc agccgugacg cucccuucuc      120

acucuacaag gaaguugcaa acgcggucgc agaccugguu agaaucaaga gaauacacga      180

agcacuugau caagguugaa aacuggauau ucaggaaccc cggguuugcg cuaguggccg      240

uugccauugc cuggcuuuug ggaagcucga cgagccaaaa agucauauac uuggucauga      300

uacugcugau ugccccggca uacaguauca ggugcauugg agucagcaau agagacuucg      360

uggagggcau gucagguggg accuggguug auguugucuu ggaacaugga ggcugcguua      420

ccgugauggc acaggacaag ccaacagucg acauagaguu ggucacgacg acgguuagua      480

acauggccga gguaagaucc uauugcuacg aggcaucgau aucggacaug gcuucggaca      540

gucguugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      600

gcaaaagaac auuaguggac agagguuggg gaaacgguug uggacuuuuu ggcaaaggga      660

gcuuggugac augugccaag uuuacguguu cuaagaagau gaccgggaag agcauucaac      720

cggaaaaucu ggaguaucgg auaaugcuau cagugcaugg cucccagcau agcgggauga      780

uuggauauga aacugacgaa gauagagcga aagucgaggu uacgccuaau ucaccaagag      840

cggaagcaac cuugggaggc uuuggaaguu uaggacuuga cugugaacca aggacaggcc      900

uugacuuuuc agaucuguau uaccugacca ugaacaauaa gcauugguug gugcacaaag      960

agugguuuca ugacauccca uugccuuggc augcuggggc agacaccgga acuccacacu     1020

ggaacaacaa agaggcauug guagaauuua aggaugccca cgccaagagg caaaccgucg     1080

ucguucuggg gagccaggaa ggagccguuc acacggcucu cgcuggagcu cuagaggcug     1140

agauggaugg ugcaaaggga aggcuguucu cuggccauuu gaaaugccgc cuaaaaaugg     1200

acaagcucag auugaagggc gugucauauu ccuugugcac ugcggcauuc acauucacca     1260

aggucccagc ugaaacacug cauggaacag ucacagugga ggugcaguau gcagggacag     1320

auggacccug caagauccca guccagaugg cgguggacau gcagacccug accccaguug     1380

gaaggcugau aaccgccaac cccgugauua cugaaagcac ugagaacuca aagaugaugu     1440

uggagcuuga cccaccauuu ggggauucuu acauugucau aggaguuggg gacaagaaaa     1500

ucacccacca cuggcauagg agugguagca ccaucggaaa ggcauuugag gccacuguga     1560

gaggcgccaa gagaauggca guccuggggg auacagccug ggacuucgga ucagucgggg     1620

guguguucaa cucacugggu aagggcauuc accagauuuu uggagcagcc uucaaaucac     1680

uguuuggagg aauguccugg uucucacaga uccucauagg cacgcugcua gugugguuag     1740

guuugaacac aaagaaugga ucuaucuccc ucacaugcuu ggcccugggg ggagugauga     1800

ucuuccucuc cacggcuguu ucugcuugag gacuagugca ucacauuuaa aagcaucuca     1860

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     1920

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     1980

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2100

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2158


<210>  10348
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 wild type

<400>  10348
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gugucggaau uguuggccuc cugcugacca cagcuauggc agcugugacg cuccccuccc      120

auuccacuag gaagcugcaa acgcggucgc aaaccugguu ggaaucaaga gaauacacaa      180

agcacuugau uagagucgaa aauuggauau ucaggaaccc uggcuucgcg uuagcagcag      240

cugccaucgc uuggcuuuug ggaagcucaa cgagccaaaa agucauauac uuggucauga      300

uacugcugau ugccccggca uacagcauca ggugcauagg agucagcaau agggacuuug      360

uggaagguau gucagguggg acuuggguug auguugucuu ggaacaugga gguuguguca      420

ccguaauggc acaggacaaa ccgacugucg acauagagcu gguuacaaca acagucagca      480

acauggcgga gguaagaucc uacugcuaug aggcaucaau aucagacaug gcuucggaca      540

gccgcugccc aacacaaggu gaagccuacc uugacaagca aucagacacu caauaugucu      600

gcaaaagaac guuaguggac agaggcuggg gaaauggaug uggacuuuuu ggcaaaggga      660

gccuggugac augcgcuaag uuugcaugcu ccaagaaaau gaccgggaag agcauccagc      720

cagagaaucu ggaguaccgg auaaugcugu caguucaugg cucccagcac agugggauga      780

ucguuaauga cacaggacau gaaacugaug agaauagagc gaagguugag auaacgccca      840

auucaccaag agccgaagcc acccuggggg guuuuggaag ccuaggacuu gauugugaac      900

cgaggacagg ccuugacuuu ucagauuugu auuacuugac uaugaauaac aagcacuggu      960

ugguccacaa ggagugguuc cacgacauuc cauuaccuug gcacgcuggg gcagacaccg     1020

gaacuccaca cuggaacaac aaagaagcac ugguagaguu caaggacgca caugccaaaa     1080

ggcaaacugu cgugguucua gggagucaag aaggagcagu ucacacggcc cuugcuggag     1140

cucuggaggc ugagauggau ggugcaaagg gaaggcuguc cucuggccac uugaaauguc     1200

gccugaaaau ggauaaacuu agauugaagg gcgugucaua cuccuugugu accgcagcgu     1260

ucacauucac caagaucccg gcugaaacac ugcacgggac agucacagug gagguacagu     1320

acgcagggac agauggaccu ugcaagguuc cagcucagau ggcgguggac augcaaacuc     1380

ugaccccagu ugggagguug auaaccgcua accccguaau cacugaaagc acugagaacu     1440

cuaagaugau gcuggaacuu gauccaccau uuggggacuc uuacauuguc auaggagucg     1500

gggagaagaa gaucacccac cacuggcaca ggaguggcag caccauugga aaagcauuug     1560

aagccacugu gagaggugcc aagagaaugg cagucuuggg agacacagcc ugggacuuug     1620

gaucaguugg aggcgcucuc aacucauugg gcaagggcau ccaucaaauu uuuggagcag     1680

cuuucaaauc auuguuugga ggaauguccu gguucucaca aauccucauu ggaacguugc     1740

ugaugugguu gggucugaac acaaagaaug gaucuauuuc ccuuaugugc uuggccuuag     1800

ggggaguguu gaucuucuua uccacagccg ucucugcuug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10349
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 wild type

<400>  10349
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa      180

ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau uggauauuca      240

ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga agcucaacga      300

gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac agcaucaggu      360

gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu uggguugaua      420

uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg acugucgaca      480

uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac ugcuaugagg      540

caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa gccuaccuug      600

acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga ggcuggggaa      660

auggaugugg acuuuuuggc aaagggaguc uggugacaug cgcuaaguuu gcaugcucca      720

agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua augcugucag      780

uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa acugaugaga      840

auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc cugggggguu      900

uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca gauuuguauu      960

acuugacuau gaauaacaag cacugguugg uucacaagga gugguuccac gacauuccau     1020

uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa gaagcacugg     1080

uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg agucaagaag     1140

gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu gcaaagggaa     1200

ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg     1260

ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu gaaacacugc     1320

acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc aagguuccag     1380

cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua accgcuaacc     1440

ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau ccaccauuug     1500

gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac uggcacagga     1560

guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag agaauggcag     1620

ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac ucauugggca     1680

agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga auguccuggu     1740

ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacaca aagaauggau     1800

cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc acagccgucu     1860

cugcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10350
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 wild type

<400>  10350
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa      180

ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau uggauauuca      240

ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga agcucaacga      300

gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac agcaucaggu      360

gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu uggguugaug      420

uugucuugga acauggaggu ugugucacug uaauggcaca ggacaaaccg acugucgaca      480

uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac ugcuaugagg      540

caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa gccuaccuug      600

acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga ggcuggggaa      660

auggaugugg acuuuuuggc aaagggagcc uggugacaug cgcuaaguuu gcaugcucca      720

agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua augcugucag      780

uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa acugaugaga      840

auagagcgaa aguugagaua acgcccaauu caccaagagc cgaagccacc cugggggggu      900

uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca gauuuguauu      960

acuugacuau gaauaacaag cacuggcugg uucacaagga gugguuccac gacauuccau     1020

uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa gaagcacugg     1080

uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg agucaagaag     1140

gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu gcaaagggaa     1200

ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg     1260

ugucauacuc cuuguguacu gcagcguuca cauucaccaa gaucccggcu gaaacacugc     1320

acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc aagguuccag     1380

cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua accgcuaacc     1440

ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau ccaccauuug     1500

gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac uggcacagga     1560

guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag agaauggcag     1620

ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac ucauugggca     1680

agggcaucca ucaaaucuuu ggagcagcuu ucaaaucauu guuuggagga auguccuggu     1740

ucucacaaau ucucauugga acguugcuga ugugguuggg ucugaacgca aagaauggau     1800

cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc acagccgucu     1860

cugcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10351
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 wild type

<400>  10351
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugc cgugacgcuc ccuucucacu cuacaaggaa guugcaaacg cggucgcaga      180

ccugguuaga aucaagagaa uacacgaagc acuugaucaa gguugaaaac uggauauuca      240

ggaaccccgg guuugcgcua guggccguug ccauugccug gcuuuuggga agcucgacga      300

gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac aguaucaggu      360

gcauuggagu cagcaauaga gacuucgugg agggcauguc aggugggacc uggguugaug      420

uugucuugga acauggaggc ugcguuaccg ugauggcaca ggacaagcca acagucgaca      480

uagaguuggu cacgacgacg guuaguaaca uggccgaggu aagauccuau ugcuacgagg      540

caucgauauc ggacauggcu ucggacaguc guugcccaac acaaggugaa gccuaccuug      600

acaagcaauc agacacucaa uaugucugca aaagaacauu aguggacaga gguuggggaa      660

acgguugugg acuuuuuggc aaagggagcu uggugacaug ugccaaguuu acguguucua      720

agaagaugac cgggaagagc auucaaccgg aaaaucugga guaucggaua augcuaucag      780

ugcauggcuc ccagcauagc gggaugauug gauaugaaac ugacgaagau agagcgaaag      840

ucgagguuac gccuaauuca ccaagagcgg aagcaaccuu gggaggcuuu ggaaguuuag      900

gacuugacug ugaaccaagg acaggccuug acuuuucaga ucuguauuac cugaccauga      960

acaauaagca uugguuggug cacaaagagu gguuucauga caucccauug ccuuggcaug     1020

cuggggcaga caccggaacu ccacacugga acaacaaaga ggcauuggua gaauuuaagg     1080

augcccacgc caagaggcaa accgucgucg uucuggggag ccaggaagga gccguucaca     1140

cggcucucgc uggagcucua gaggcugaga uggauggugc aaagggaagg cuguucucug     1200

gccauuugaa augccgccua aaaauggaca agcucagauu gaagggcgug ucauauuccu     1260

ugugcacugc ggcauucaca uucaccaagg ucccagcuga aacacugcau ggaacaguca     1320

caguggaggu gcaguaugca gggacagaug gacccugcaa gaucccaguc cagauggcgg     1380

uggacaugca gacccugacc ccaguuggaa ggcugauaac cgccaacccc gugauuacug     1440

aaagcacuga gaacucaaag augauguugg agcuugaccc accauuuggg gauucuuaca     1500

uugucauagg aguuggggac aagaaaauca cccaccacug gcauaggagu gguagcacca     1560

ucggaaaggc auuugaggcc acugugagag gcgccaagag aauggcaguc cugggggaua     1620

cagccuggga cuucggauca gucgggggug uguucaacuc acuggguaag ggcauucacc     1680

agauuuuugg agcagccuuc aaaucacugu uuggaggaau guccugguuc ucacagaucc     1740

ucauaggcac gcugcuagug ugguuagguu ugaacacaaa gaauggaucu aucucccuca     1800

caugcuuggc ccugggggga gugaugaucu uccucuccac ggcuguuucu gcuugaggac     1860

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     1920

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     1980

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2040

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2160

ggcucuuuuc agagccacca gaauu                                           2185


<210>  10352
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 wild type

<400>  10352
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu cccugugcua ggauuuuuca ucauagcugu gcugaugagc gcucaggaau      120

caugggcugc ugugacgcuc cccucccauu ccacuaggaa gcugcaaacg cggucgcaaa      180

ccugguugga aucaagagaa uacacaaagc acuugauuag agucgaaaau uggauauuca      240

ggaacccugg cuucgcguua gcagcagcug ccaucgcuug gcuuuuggga agcucaacga      300

gccaaaaagu cauauacuug gucaugauac ugcugauugc cccggcauac agcaucaggu      360

gcauaggagu cagcaauagg gacuuugugg aagguauguc aggugggacu uggguugaug      420

uugucuugga acauggaggu ugugucaccg uaauggcaca ggacaaaccg acugucgaca      480

uagagcuggu uacaacaaca gucagcaaca uggcggaggu aagauccuac ugcuaugagg      540

caucaauauc agacauggcu ucggacagcc gcugcccaac acaaggugaa gccuaccuug      600

acaagcaauc agacacucaa uaugucugca aaagaacguu aguggacaga ggcuggggaa      660

auggaugugg acuuuuuggc aaagggagcc uggugacaug cgcuaaguuu gcaugcucca      720

agaaaaugac cgggaagagc auccagccag agaaucugga guaccggaua augcugucag      780

uucauggcuc ccagcacagu gggaugaucg uuaaugacac aggacaugaa acugaugaga      840

auagagcgaa gguugagaua acgcccaauu caccaagagc cgaagccacc cugggggguu      900

uuggaagccu aggacuugau ugugaaccga ggacaggccu ugacuuuuca gauuuguauu      960

acuugacuau gaauaacaag cacugguugg uccacaagga gugguuccac gacauuccau     1020

uaccuuggca cgcuggggca gacaccggaa cuccacacug gaacaacaaa gaagcacugg     1080

uagaguucaa ggacgcacau gccaaaaggc aaacugucgu gguucuaggg agucaagaag     1140

gagcaguuca cacggcccuu gcuggagcuc uggaggcuga gauggauggu gcaaagggaa     1200

ggcuguccuc uggccacuug aaaugucgcc ugaaaaugga uaaacuuaga uugaagggcg     1260

ugucauacuc cuuguguacc gcagcguuca cauucaccaa gaucccggcu gaaacacugc     1320

acgggacagu cacaguggag guacaguacg cagggacaga uggaccuugc aagguuccag     1380

cucagauggc gguggacaug caaacucuga ccccaguugg gagguugaua accgcuaacc     1440

ccguaaucac ugaaagcacu gagaacucua agaugaugcu ggaacuugau ccaccauuug     1500

gggacucuua cauugucaua ggagucgggg agaagaagau cacccaccac uggcacagga     1560

guggcagcac cauuggaaaa gcauuugaag ccacugugag aggugccaag agaauggcag     1620

ucuugggaga cacagccugg gacuuuggau caguuggagg cgcucucaac ucauugggca     1680

agggcaucca ucaaauuuuu ggagcagcuu ucaaaucauu guuuggagga auguccuggu     1740

ucucacaaau ccucauugga acguugcuga ugugguuggg ucugaacaca aagaauggau     1800

cuauuucccu uaugugcuug gccuuagggg gaguguugau cuucuuaucc acagccgucu     1860

cugcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10353
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 wild type

<400>  10353
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcgcu gugacgcucc      120

ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau      180

acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc uucgcguuag      240

cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg      300

ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc agcaauaggg      360

acuuugugga agguauguca ggugggacuu ggguugauau ugucuuggaa cauggagguu      420

gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu acaacaacag      480

ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca gacauggcuu      540

cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca gacacucaau      600

augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga cuuuuuggca      660

aagggagucu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc gggaagagca      720

uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc cagcacagug      780

ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag guugagauaa      840

cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua ggacuugauu      900

gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug aauaacaagc      960

acugguuggu ucacaaggag ugguuccacg acauuccauu accuuggcac gcuggggcag     1020

acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag gacgcacaug     1080

ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac acggcccuug     1140

cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu ggccacuuga     1200

aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc uuguguaccg     1260

cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc acaguggagg     1320

uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg guggacaugc     1380

aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu gaaagcacug     1440

agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac auugucauag     1500

gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc auuggaaaag     1560

cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac acagccuggg     1620

acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau caaauuuuug     1680

gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu cucauuggaa     1740

cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu augugcuugg     1800

ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcuugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10354
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 wild type

<400>  10354
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcgcu gugacgcucc      120

ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau      180

acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc uucgcguuag      240

cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg      300

ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc agcaauaggg      360

acuuugugga agguauguca ggugggacuu ggguugaugu ugucuuggaa cauggagguu      420

gugucacugu aauggcacag gacaaaccga cugucgacau agagcugguu acaacaacag      480

ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca gacauggcuu      540

cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca gacacucaau      600

augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga cuuuuuggca      660

aagggagccu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc gggaagagca      720

uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc cagcacagug      780

ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaaa guugagauaa      840

cgcccaauuc accaagagcc gaagccaccc uggggggguu uggaagccua ggacuugauu      900

gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug aauaacaagc      960

acuggcuggu ucacaaggag ugguuccacg acauuccauu accuuggcac gcuggggcag     1020

acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag gacgcacaug     1080

ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac acggcccuug     1140

cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu ggccacuuga     1200

aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc uuguguacug     1260

cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc acaguggagg     1320

uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg guggacaugc     1380

aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu gaaagcacug     1440

agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac auugucauag     1500

gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc auuggaaaag     1560

cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac acagccuggg     1620

acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau caaaucuuug     1680

gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauu cucauuggaa     1740

cguugcugau gugguugggu cugaacgcaa agaauggauc uauuucccuu augugcuugg     1800

ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcuugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10355
<211>  2164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 wild type

<400>  10355
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcgcc gugacgcucc      120

cuucucacuc uacaaggaag uugcaaacgc ggucgcagac cugguuagaa ucaagagaau      180

acacgaagca cuugaucaag guugaaaacu ggauauucag gaaccccggg uuugcgcuag      240

uggccguugc cauugccugg cuuuugggaa gcucgacgag ccaaaaaguc auauacuugg      300

ucaugauacu gcugauugcc ccggcauaca guaucaggug cauuggaguc agcaauagag      360

acuucgugga gggcauguca ggugggaccu ggguugaugu ugucuuggaa cauggaggcu      420

gcguuaccgu gauggcacag gacaagccaa cagucgacau agaguugguc acgacgacgg      480

uuaguaacau ggccgaggua agauccuauu gcuacgaggc aucgauaucg gacauggcuu      540

cggacagucg uugcccaaca caaggugaag ccuaccuuga caagcaauca gacacucaau      600

augucugcaa aagaacauua guggacagag guuggggaaa cgguugugga cuuuuuggca      660

aagggagcuu ggugacaugu gccaaguuua cguguucuaa gaagaugacc gggaagagca      720

uucaaccgga aaaucuggag uaucggauaa ugcuaucagu gcauggcucc cagcauagcg      780

ggaugauugg auaugaaacu gacgaagaua gagcgaaagu cgagguuacg ccuaauucac      840

caagagcgga agcaaccuug ggaggcuuug gaaguuuagg acuugacugu gaaccaagga      900

caggccuuga cuuuucagau cuguauuacc ugaccaugaa caauaagcau ugguuggugc      960

acaaagagug guuucaugac aucccauugc cuuggcaugc uggggcagac accggaacuc     1020

cacacuggaa caacaaagag gcauugguag aauuuaagga ugcccacgcc aagaggcaaa     1080

ccgucgucgu ucuggggagc caggaaggag ccguucacac ggcucucgcu ggagcucuag     1140

aggcugagau ggauggugca aagggaaggc uguucucugg ccauuugaaa ugccgccuaa     1200

aaauggacaa gcucagauug aagggcgugu cauauuccuu gugcacugcg gcauucacau     1260

ucaccaaggu cccagcugaa acacugcaug gaacagucac aguggaggug caguaugcag     1320

ggacagaugg acccugcaag aucccagucc agauggcggu ggacaugcag acccugaccc     1380

caguuggaag gcugauaacc gccaaccccg ugauuacuga aagcacugag aacucaaaga     1440

ugauguugga gcuugaccca ccauuugggg auucuuacau ugucauagga guuggggaca     1500

agaaaaucac ccaccacugg cauaggagug guagcaccau cggaaaggca uuugaggcca     1560

cugugagagg cgccaagaga auggcagucc ugggggauac agccugggac uucggaucag     1620

ucgggggugu guucaacuca cuggguaagg gcauucacca gauuuuugga gcagccuuca     1680

aaucacuguu uggaggaaug uccugguucu cacagauccu cauaggcacg cugcuagugu     1740

gguuagguuu gaacacaaag aauggaucua ucucccucac augcuuggcc cuggggggag     1800

ugaugaucuu ccucuccacg gcuguuucug cuugaggacu agugcaucac auuuaaaagc     1860

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     1920

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     1980

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2160

aauu                                                                  2164


<210>  10356
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 wild type

<400>  10356
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcgcu gugacgcucc      120

ccucccauuc cacuaggaag cugcaaacgc ggucgcaaac cugguuggaa ucaagagaau      180

acacaaagca cuugauuaga gucgaaaauu ggauauucag gaacccuggc uucgcguuag      240

cagcagcugc caucgcuugg cuuuugggaa gcucaacgag ccaaaaaguc auauacuugg      300

ucaugauacu gcugauugcc ccggcauaca gcaucaggug cauaggaguc agcaauaggg      360

acuuugugga agguauguca ggugggacuu ggguugaugu ugucuuggaa cauggagguu      420

gugucaccgu aauggcacag gacaaaccga cugucgacau agagcugguu acaacaacag      480

ucagcaacau ggcggaggua agauccuacu gcuaugaggc aucaauauca gacauggcuu      540

cggacagccg cugcccaaca caaggugaag ccuaccuuga caagcaauca gacacucaau      600

augucugcaa aagaacguua guggacagag gcuggggaaa uggaugugga cuuuuuggca      660

aagggagccu ggugacaugc gcuaaguuug caugcuccaa gaaaaugacc gggaagagca      720

uccagccaga gaaucuggag uaccggauaa ugcugucagu ucauggcucc cagcacagug      780

ggaugaucgu uaaugacaca ggacaugaaa cugaugagaa uagagcgaag guugagauaa      840

cgcccaauuc accaagagcc gaagccaccc ugggggguuu uggaagccua ggacuugauu      900

gugaaccgag gacaggccuu gacuuuucag auuuguauua cuugacuaug aauaacaagc      960

acugguuggu ccacaaggag ugguuccacg acauuccauu accuuggcac gcuggggcag     1020

acaccggaac uccacacugg aacaacaaag aagcacuggu agaguucaag gacgcacaug     1080

ccaaaaggca aacugucgug guucuaggga gucaagaagg agcaguucac acggcccuug     1140

cuggagcucu ggaggcugag auggauggug caaagggaag gcuguccucu ggccacuuga     1200

aaugucgccu gaaaauggau aaacuuagau ugaagggcgu gucauacucc uuguguaccg     1260

cagcguucac auucaccaag aucccggcug aaacacugca cgggacaguc acaguggagg     1320

uacaguacgc agggacagau ggaccuugca agguuccagc ucagauggcg guggacaugc     1380

aaacucugac cccaguuggg agguugauaa ccgcuaaccc cguaaucacu gaaagcacug     1440

agaacucuaa gaugaugcug gaacuugauc caccauuugg ggacucuuac auugucauag     1500

gagucgggga gaagaagauc acccaccacu ggcacaggag uggcagcacc auuggaaaag     1560

cauuugaagc cacugugaga ggugccaaga gaauggcagu cuugggagac acagccuggg     1620

acuuuggauc aguuggaggc gcucucaacu cauugggcaa gggcauccau caaauuuuug     1680

gagcagcuuu caaaucauug uuuggaggaa uguccugguu cucacaaauc cucauuggaa     1740

cguugcugau gugguugggu cugaacacaa agaauggauc uauuucccuu augugcuugg     1800

ccuuaggggg aguguugauc uucuuaucca cagccgucuc ugcuugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10357
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 wild type

<400>  10357
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      180

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      240

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      300

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      360

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauauug      420

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      480

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      540

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      600

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      660

gauguggacu uuuuggcaaa gggagucugg ugacaugcgc uaaguuugca ugcuccaaga      720

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      780

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      840

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      900

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      960

ugacuaugaa uaacaagcac ugguugguuc acaaggagug guuccacgac auuccauuac     1020

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1080

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1140

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1200

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1260

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1320

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1380

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1440

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1500

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1560

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1620

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1680

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     1740

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     1800

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     1860

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10358
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 wild type

<400>  10358
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      180

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      240

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      300

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      360

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      420

ucuuggaaca uggagguugu gucacuguaa uggcacagga caaaccgacu gucgacauag      480

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      540

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      600

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      660

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      720

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      780

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      840

gagcgaaagu ugagauaacg cccaauucac caagagccga agccacccug gggggguuug      900

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      960

ugacuaugaa uaacaagcac uggcugguuc acaaggagug guuccacgac auuccauuac     1020

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1080

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1140

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1200

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1260

cauacuccuu guguacugca gcguucacau ucaccaagau cccggcugaa acacugcacg     1320

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1380

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1440

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1500

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1560

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1620

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1680

gcauccauca aaucuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     1740

cacaaauucu cauuggaacg uugcugaugu gguugggucu gaacgcaaag aauggaucua     1800

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     1860

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10359
<211>  2182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 wild type

<400>  10359
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgccgu gacgcucccu ucucacucua caaggaaguu gcaaacgcgg ucgcagaccu      180

gguuagaauc aagagaauac acgaagcacu ugaucaaggu ugaaaacugg auauucagga      240

accccggguu ugcgcuagug gccguugcca uugccuggcu uuugggaagc ucgacgagcc      300

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagu aucaggugca      360

uuggagucag caauagagac uucguggagg gcaugucagg ugggaccugg guugauguug      420

ucuuggaaca uggaggcugc guuaccguga uggcacagga caagccaaca gucgacauag      480

aguuggucac gacgacgguu aguaacaugg ccgagguaag auccuauugc uacgaggcau      540

cgauaucgga cauggcuucg gacagucguu gcccaacaca aggugaagcc uaccuugaca      600

agcaaucaga cacucaauau gucugcaaaa gaacauuagu ggacagaggu uggggaaacg      660

guuguggacu uuuuggcaaa gggagcuugg ugacaugugc caaguuuacg uguucuaaga      720

agaugaccgg gaagagcauu caaccggaaa aucuggagua ucggauaaug cuaucagugc      780

auggcuccca gcauagcggg augauuggau augaaacuga cgaagauaga gcgaaagucg      840

agguuacgcc uaauucacca agagcggaag caaccuuggg aggcuuugga aguuuaggac      900

uugacuguga accaaggaca ggccuugacu uuucagaucu guauuaccug accaugaaca      960

auaagcauug guuggugcac aaagaguggu uucaugacau cccauugccu uggcaugcug     1020

gggcagacac cggaacucca cacuggaaca acaaagaggc auugguagaa uuuaaggaug     1080

cccacgccaa gaggcaaacc gucgucguuc uggggagcca ggaaggagcc guucacacgg     1140

cucucgcugg agcucuagag gcugagaugg auggugcaaa gggaaggcug uucucuggcc     1200

auuugaaaug ccgccuaaaa auggacaagc ucagauugaa gggcguguca uauuccuugu     1260

gcacugcggc auucacauuc accaaggucc cagcugaaac acugcaugga acagucacag     1320

uggaggugca guaugcaggg acagauggac ccugcaagau cccaguccag auggcggugg     1380

acaugcagac ccugacccca guuggaaggc ugauaaccgc caaccccgug auuacugaaa     1440

gcacugagaa cucaaagaug auguuggagc uugacccacc auuuggggau ucuuacauug     1500

ucauaggagu uggggacaag aaaaucaccc accacuggca uaggaguggu agcaccaucg     1560

gaaaggcauu ugaggccacu gugagaggcg ccaagagaau ggcaguccug ggggauacag     1620

ccugggacuu cggaucaguc gggggugugu ucaacucacu ggguaagggc auucaccaga     1680

uuuuuggagc agccuucaaa ucacuguuug gaggaauguc cugguucuca cagauccuca     1740

uaggcacgcu gcuagugugg uuagguuuga acacaaagaa uggaucuauc ucccucacau     1800

gcuuggcccu ggggggagug augaucuucc ucuccacggc uguuucugcu ugaggacuag     1860

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     1920

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     1980

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2040

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2160

ucuuuucaga gccaccagaa uu                                              2182


<210>  10360
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 wild type

<400>  10360
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aacggagcgc uggcucaauc auguggcucg cgagcuuggc aguugucaua gcuugugcag      120

gagccgcugu gacgcucccc ucccauucca cuaggaagcu gcaaacgcgg ucgcaaaccu      180

gguuggaauc aagagaauac acaaagcacu ugauuagagu cgaaaauugg auauucagga      240

acccuggcuu cgcguuagca gcagcugcca ucgcuuggcu uuugggaagc ucaacgagcc      300

aaaaagucau auacuugguc augauacugc ugauugcccc ggcauacagc aucaggugca      360

uaggagucag caauagggac uuuguggaag guaugucagg ugggacuugg guugauguug      420

ucuuggaaca uggagguugu gucaccguaa uggcacagga caaaccgacu gucgacauag      480

agcugguuac aacaacaguc agcaacaugg cggagguaag auccuacugc uaugaggcau      540

caauaucaga cauggcuucg gacagccgcu gcccaacaca aggugaagcc uaccuugaca      600

agcaaucaga cacucaauau gucugcaaaa gaacguuagu ggacagaggc uggggaaaug      660

gauguggacu uuuuggcaaa gggagccugg ugacaugcgc uaaguuugca ugcuccaaga      720

aaaugaccgg gaagagcauc cagccagaga aucuggagua ccggauaaug cugucaguuc      780

auggcuccca gcacaguggg augaucguua augacacagg acaugaaacu gaugagaaua      840

gagcgaaggu ugagauaacg cccaauucac caagagccga agccacccug ggggguuuug      900

gaagccuagg acuugauugu gaaccgagga caggccuuga cuuuucagau uuguauuacu      960

ugacuaugaa uaacaagcac ugguuggucc acaaggagug guuccacgac auuccauuac     1020

cuuggcacgc uggggcagac accggaacuc cacacuggaa caacaaagaa gcacugguag     1080

aguucaagga cgcacaugcc aaaaggcaaa cugucguggu ucuagggagu caagaaggag     1140

caguucacac ggcccuugcu ggagcucugg aggcugagau ggauggugca aagggaaggc     1200

uguccucugg ccacuugaaa ugucgccuga aaauggauaa acuuagauug aagggcgugu     1260

cauacuccuu guguaccgca gcguucacau ucaccaagau cccggcugaa acacugcacg     1320

ggacagucac aguggaggua caguacgcag ggacagaugg accuugcaag guuccagcuc     1380

agauggcggu ggacaugcaa acucugaccc caguugggag guugauaacc gcuaaccccg     1440

uaaucacuga aagcacugag aacucuaaga ugaugcugga acuugaucca ccauuugggg     1500

acucuuacau ugucauagga gucggggaga agaagaucac ccaccacugg cacaggagug     1560

gcagcaccau uggaaaagca uuugaagcca cugugagagg ugccaagaga auggcagucu     1620

ugggagacac agccugggac uuuggaucag uuggaggcgc ucucaacuca uugggcaagg     1680

gcauccauca aauuuuugga gcagcuuuca aaucauuguu uggaggaaug uccugguucu     1740

cacaaauccu cauuggaacg uugcugaugu gguugggucu gaacacaaag aauggaucua     1800

uuucccuuau gugcuuggcc uuagggggag uguugaucuu cuuauccaca gccgucucug     1860

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10361
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10361
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgu cacccggcgc ggcuccgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg ggcugcguga      720

ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg accgugagca      780

acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgugu      900

gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac uccgggauga     1080

ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg cacgccaagc     1380

gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug gaggugcagu     1620

acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac augcagaccc     1680

ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc accgagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgugg     1800

gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg     1920

gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacgcugc     2040

ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc cucgcccugg     2100

gcggcguccu gaucuuccuc uccaccgcgg ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10362
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10362
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgu cacccggcgc ggcuccgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca cacgugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccacgag caccuggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacgcg caagcugcag acccggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca cgagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc accugggugg acgucgugcu ggagcacggg ggcugcguga      720

ccgucauggc ccaggacaag cccaccgugg acaucgagcu cgugacgacc accgucagca      780

acauggccga ggugcgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacg caguacgucu      900

gcaagcgcac ccugguggac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccguccacgg cagccagcac uccgggauga     1080

ucgugaacga cacgggccac gagaccgacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggacggg ccuggacuuc agcgaccugu acuaccucac caugaacaac aagcacuggc     1260

ugguccacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucguggaguu caaggacgcg cacgccaagc     1380

gccagacggu ggucgugcug ggcucccagg agggggccgu gcacaccgcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggucagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagacgc uccacggcac cgugaccgug gagguccagu     1620

acgccgggac cgacggcccg ugcaaggugc ccgcccagau ggccguggac augcagacgc     1680

ugacccccgu cgggcgccug aucaccgcga accccgugau caccgagagc acggagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgucg     1800

gggagaagaa gaucacccac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1860

aggccaccgu gcgcggcgcc aagcggaugg ccgugcuggg ggacacggcg ugggacuucg     1920

gcagcgucgg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacccugc     2040

ugauguggcu cgggcugaac gccaagaacg gcuccaucag ccugaugugc cucgcgcugg     2100

gcggcgugcu gaucuuccuc uccaccgccg ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10363
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10363
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccau cacccgccgg ggguccgccu acuacaugua ccuggaccgc agcgacgccg      180

gcaaggccau cuccuucgcg accacgcugg gcgugaacaa gugccacgug cagaucaugg      240

accucgggca caugugcgac gccaccauga gcuacgagug cccgaugcug gacgagggcg      300

ucgagcccga cgacguggac ugcuggugca acaccaccuc cacgugggug gucuacggga      360

ccugccacca caagaagggc gaggcccggc gcagccggcg cgccgugacc cugcccuccc      420

acagcacccg gaagcuccag acgcgcuccc agaccuggcu ggagagccgc gaguacacca      480

agcaccugau caagguggag aacuggaucu uccggaaccc cggcuucgcc cucgucgcgg      540

uggccaucgc cuggcugcug ggguccagca ccucccagaa ggugaucuac cucgucauga      600

uccugcugau cgccccggcc uacagcaucc gcugcaucgg cguguccaac cgggacuucg      660

uggaggggau gagcggcggg acgugggucg acguggugcu cgagcacggc ggcugcguca      720

ccgugauggc gcaggacaag cccaccgugg acaucgagcu ggucaccacg accgugucca      780

acauggccga gguccgcagc uacugcuacg aggccagcau cuccgacaug gccagcgacu      840

cccggugccc cacccagggg gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagcgcac gcucguggac cggggcuggg ggaacggcug cgggcuguuc ggcaagggcu      960

cccuggucac gugcgcgaag uucacgugca gcaagaagau gaccgggaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcuga gcgugcacgg cucccagcac agcgggauga     1080

ucggcuacga gacggacgag gaccgggcca agguggaggu caccccgaac uccccgcgcg     1140

ccgaggccac ccugggcggg uucggcagcc ucgggcugga cugcgagccc cggaccggcc     1200

uggacuucuc cgaccucuac uaccugacga ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cucccguggc acgcgggggc cgacaccggc accccgcacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagcgc cagaccgucg     1380

uggugcuggg cagccaggag ggggcggucc acacggcccu ggccggcgcc cucgaggccg     1440

agauggacgg ggcgaagggc cggcuguucu ccgggcaccu gaagugccgc cucaagaugg     1500

acaagcugcg gcugaagggc gugagcuacu cccucugcac cgccgccuuc accuucacca     1560

aggugcccgc cgagacccug cacggcacgg ucaccgugga ggugcaguac gccgggaccg     1620

acggccccug caagaucccg guccagaugg cgguggacau gcagacccug acgcccgugg     1680

ggcgccucau caccgccaac cccgucauca ccgagagcac cgagaacucc aagaugaugc     1740

uggagcugga cccgccguuc ggcgacagcu acaucgugau cggggugggc gacaagaaga     1800

ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag gccaccgucc     1860

gcggcgcgaa gcggauggcc gugcucgggg acaccgccug ggacuucggc agcgugggcg     1920

gcgucuucaa cucccugggg aaggggaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggggg cauguccugg uucagccaga uccucaucgg gacgcugcug guguggcucg     2040

gccugaacac caagaacggc uccaucagcc ugaccugccu cgcgcugggc ggcgugauga     2100

ucuuccuguc caccgccguc agcgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10364
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10364
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgu cacccggcgc ggcuccgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc acgugggugg acgucgugcu ggagcacggg ggcugcguga      720

ccgucauggc ccaggacaag cccaccgugg acaucgagcu cgugaccacg accgucagca      780

acauggccga ggugcgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgucu      900

gcaagcgcac gcugguggac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccguccacgg cagccagcac uccgggauga     1080

ucgugaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc     1260

ugguccacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucguggaguu caaggacgcg cacgccaagc     1380

gccagaccgu ggucgugcug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggucagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagaccc uccacggcac ggugaccgug gagguccagu     1620

acgccgggac cgacggcccg ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacgcccgu cgggcgccug aucaccgcga accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgucg     1800

gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1860

aggccaccgu gcgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg     1920

gcagcgucgg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacgcugc     2040

ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc cucgcccugg     2100

gcggcgugcu gaucuuccuc uccaccgcgg ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10365
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10365
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccguc cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgugggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcguccug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10366
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10366
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac acgugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccacgagc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacgcgc aagcugcaga      420

cccggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac gagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugacgacca ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacacgc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac acgggccacg     1080

agaccgacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggacgggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagacggug gucgugcugg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacgcu ccacggcacc gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacgcu gacccccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca cggagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacacggcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacccugcu gauguggcuc gggcugaacg     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcgcuggg cggcgugcug aucuuccucu     2100

ccaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10367
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10367
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcauc acccgccggg      120

gguccgccua cuacauguac cuggaccgca gcgacgccgg caaggccauc uccuucgcga      180

ccacgcuggg cgugaacaag ugccacgugc agaucaugga ccucgggcac augugcgacg      240

ccaccaugag cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccucc acgugggugg ucuacgggac cugccaccac aagaagggcg      360

aggcccggcg cagccggcgc gccgugaccc ugcccuccca cagcacccgg aagcuccaga      420

cgcgcuccca gaccuggcug gagagccgcg aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu ccggaacccc ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg      540

gguccagcac cucccagaag gugaucuacc ucgucaugau ccugcugauc gccccggccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggga      660

cgugggucga cguggugcuc gagcacggcg gcugcgucac cgugauggcg caggacaagc      720

ccaccgugga caucgagcug gucaccacga ccguguccaa cauggccgag guccgcagcu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagcgcacg cucguggacc      900

ggggcugggg gaacggcugc gggcuguucg gcaagggcuc ccuggucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcugag cgugcacggc ucccagcaca gcgggaugau cggcuacgag acggacgagg     1080

accgggccaa gguggagguc accccgaacu ccccgcgcgc cgaggccacc cugggcgggu     1140

ucggcagccu cgggcuggac ugcgagcccc ggaccggccu ggacuucucc gaccucuacu     1200

accugacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ucccguggca cgcgggggcc gacaccggca ccccgcacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagcgcc agaccgucgu ggugcugggc agccaggagg     1380

gggcggucca cacggcccug gccggcgccc ucgaggccga gauggacggg gcgaagggcc     1440

ggcuguucuc cgggcaccug aagugccgcc ucaagaugga caagcugcgg cugaagggcg     1500

ugagcuacuc ccucugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcacggu caccguggag gugcaguacg ccgggaccga cggccccugc aagaucccgg     1620

uccagauggc gguggacaug cagacccuga cgcccguggg gcgccucauc accgccaacc     1680

ccgucaucac cgagagcacc gagaacucca agaugaugcu ggagcuggac ccgccguucg     1740

gcgacagcua caucgugauc ggggugggcg acaagaagau cacgcaccac uggcaccgga     1800

gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgcgaag cggauggccg     1860

ugcucgggga caccgccugg gacuucggca gcgugggcgg cgucuucaac ucccugggga     1920

aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc auguccuggu     1980

ucagccagau ccucaucggg acgcugcugg uguggcucgg ccugaacacc aagaacggcu     2040

ccaucagccu gaccugccuc gcgcugggcg gcgugaugau cuuccugucc accgccguca     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10368
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10368
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugaccacga ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacg cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagaccgug gucgugcugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacgcccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcucc accaucggga aggccuucga ggccaccgug cgcggcgcca     1860

agcggauggc cgugcugggg gacaccgcgu gggacuucgg cagcgucggc ggggcccuca     1920

acucccuggg caaggggauc caccagaucu ucggcgccgc cuucaagagc cuguucgggg     1980

gcauguccug guucagccag auccucaucg gcacgcugcu gauguggcuc gggcugaaca     2040

ccaagaacgg cuccaucagc cugaugugcc ucgcccuggg cggcgugcug aucuuccucu     2100

ccaccgcggu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10369
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10369
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc ugcgugaccg      720

ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc gugagcaaca      780

uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgugugca      900

agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc gggaugaucg     1080

ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc     1380

agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag gugcaguacg     1620

ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug cagacccuga     1680

cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcguggggg     1800

agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     1860

ccaccguccg cggcgccaag cggauggccg ugcuggggga caccgcgugg gacuucggca     1920

gcgugggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acgcugcuga     2040

uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc gcccugggcg     2100

gcguccugau cuuccucucc accgcgguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10370
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10370
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacac gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccacgagcac cugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacgcgcaa gcugcagacc cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacga gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacc uggguggacg ucgugcugga gcacgggggc ugcgugaccg      720

ucauggccca ggacaagccc accguggaca ucgagcucgu gacgaccacc gucagcaaca      780

uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacgcag uacgucugca      900

agcgcacccu gguggaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc gggaugaucg     1080

ugaacgacac gggccacgag accgacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggacgggccu ggacuucagc gaccuguacu accucaccau gaacaacaag cacuggcugg     1260

uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac gccaagcgcc     1380

agacgguggu cgugcugggc ucccaggagg gggccgugca caccgcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacgcucc acggcaccgu gaccguggag guccaguacg     1620

ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug cagacgcuga     1680

cccccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacg gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcgucgggg     1800

agaagaagau cacccaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     1860

ccaccgugcg cggcgccaag cggauggccg ugcuggggga cacggcgugg gacuucggca     1920

gcgucggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acccugcuga     2040

uguggcucgg gcugaacgcc aagaacggcu ccaucagccu gaugugccuc gcgcugggcg     2100

gcgugcugau cuuccucucc accgccguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10371
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10371
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccaucac ccgccggggg uccgccuacu acauguaccu ggaccgcagc gacgccggca      180

aggccaucuc cuucgcgacc acgcugggcg ugaacaagug ccacgugcag aucauggacc      240

ucgggcacau gugcgacgcc accaugagcu acgagugccc gaugcuggac gagggcgucg      300

agcccgacga cguggacugc uggugcaaca ccaccuccac gugggugguc uacgggaccu      360

gccaccacaa gaagggcgag gcccggcgca gccggcgcgc cgugacccug cccucccaca      420

gcacccggaa gcuccagacg cgcucccaga ccuggcugga gagccgcgag uacaccaagc      480

accugaucaa gguggagaac uggaucuucc ggaaccccgg cuucgcccuc gucgcggugg      540

ccaucgccug gcugcugggg uccagcaccu cccagaaggu gaucuaccuc gucaugaucc      600

ugcugaucgc cccggccuac agcauccgcu gcaucggcgu guccaaccgg gacuucgugg      660

aggggaugag cggcgggacg ugggucgacg uggugcucga gcacggcggc ugcgucaccg      720

ugauggcgca ggacaagccc accguggaca ucgagcuggu caccacgacc guguccaaca      780

uggccgaggu ccgcagcuac ugcuacgagg ccagcaucuc cgacauggcc agcgacuccc      840

ggugccccac ccagggggag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agcgcacgcu cguggaccgg ggcuggggga acggcugcgg gcuguucggc aagggcuccc      960

uggucacgug cgcgaaguuc acgugcagca agaagaugac cgggaagucc auccagcccg     1020

agaaccucga guaccgcauc augcugagcg ugcacggcuc ccagcacagc gggaugaucg     1080

gcuacgagac ggacgaggac cgggccaagg uggaggucac cccgaacucc ccgcgcgccg     1140

aggccacccu gggcggguuc ggcagccucg ggcuggacug cgagccccgg accggccugg     1200

acuucuccga ccucuacuac cugacgauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccccuc ccguggcacg cgggggccga caccggcacc ccgcacugga     1320

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagcgccag accgucgugg     1380

ugcugggcag ccaggagggg gcgguccaca cggcccuggc cggcgcccuc gaggccgaga     1440

uggacggggc gaagggccgg cuguucuccg ggcaccugaa gugccgccuc aagauggaca     1500

agcugcggcu gaagggcgug agcuacuccc ucugcaccgc cgccuucacc uucaccaagg     1560

ugcccgccga gacccugcac ggcacgguca ccguggaggu gcaguacgcc gggaccgacg     1620

gccccugcaa gaucccgguc cagauggcgg uggacaugca gacccugacg cccguggggc     1680

gccucaucac cgccaacccc gucaucaccg agagcaccga gaacuccaag augaugcugg     1740

agcuggaccc gccguucggc gacagcuaca ucgugaucgg ggugggcgac aagaagauca     1800

cgcaccacug gcaccggagc ggcuccacca ucgggaaggc cuucgaggcc accguccgcg     1860

gcgcgaagcg gauggccgug cucggggaca ccgccuggga cuucggcagc gugggcggcg     1920

ucuucaacuc ccuggggaag gggauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucgggggcau guccugguuc agccagaucc ucaucgggac gcugcuggug uggcucggcc     2040

ugaacaccaa gaacggcucc aucagccuga ccugccucgc gcugggcggc gugaugaucu     2100

uccuguccac cgccgucagc gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10372
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10372
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacg uggguggacg ucgugcugga gcacgggggc ugcgugaccg      720

ucauggccca ggacaagccc accguggaca ucgagcucgu gaccacgacc gucagcaaca      780

uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgucugca      900

agcgcacgcu gguggaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc gggaugaucg     1080

ugaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1260

uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac gccaagcgcc     1380

agaccguggu cgugcugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacccucc acggcacggu gaccguggag guccaguacg     1620

ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cgcccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcgucgggg     1800

agaagaagau cacgcaccac uggcaccgga gcggcuccac caucgggaag gccuucgagg     1860

ccaccgugcg cggcgccaag cggauggccg ugcuggggga caccgcgugg gacuucggca     1920

gcgucggcgg ggcccucaac ucccugggca aggggaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucgggggc auguccuggu ucagccagau ccucaucggc acgcugcuga     2040

uguggcucgg gcugaacacc aagaacggcu ccaucagccu gaugugccuc gcccugggcg     2100

gcgugcugau cuuccucucc accgcgguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10373
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10373
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgu cacccggcgc ggcuccgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg ggcugcguga      720

ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg accgugagca      780

acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgugu      900

gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac uccgggauga     1080

ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg cacgccaagc     1380

gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug gaggugcagu     1620

acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac augcagaccc     1680

ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc accgagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgugg     1800

gggagaagaa gaucacgcac cacuggcacc ggagcggcag cacccugggg aaggccuucu     1860

ccaccacgcu caagggcgcc cagcgccugg cggcccuggg ggacaccgcc ugggacuucg     1920

gcagcaucgg gggcguguuc aacuccaucg gccgggccgu ccaccaggug uucgggggcg     1980

ccuuccgcac ccucuucggg ggcaugagcu ggaucaccca ggggcugaug ggcgcgcugc     2040

uccuguggau gggggugaac gcccgggacc gcuccaucgc ccuggccuuc cucgcgacgg     2100

gcggcguccu gguguuccug gccaccaacg ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10374
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10374
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgu cacccggcgc ggcuccgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca cacgugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccacgag caccuggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacgcg caagcugcag acccggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca cgagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc accugggugg acgucgugcu ggagcacggg ggcugcguga      720

ccgucauggc ccaggacaag cccaccgugg acaucgagcu cgugacgacc accgucagca      780

acauggccga ggugcgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacg caguacgucu      900

gcaagcgcac ccugguggac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccguccacgg cagccagcac uccgggauga     1080

ucgugaacga cacgggccac gagaccgacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggacggg ccuggacuuc agcgaccugu acuaccucac caugaacaac aagcacuggc     1260

ugguccacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucguggaguu caaggacgcg cacgccaagc     1380

gccagacggu ggucgugcug ggcucccagg agggggccgu gcacaccgcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggucagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagacgc uccacggcac cgugaccgug gagguccagu     1620

acgccgggac cgacggcccg ugcaaggugc ccgcccagau ggccguggac augcagacgc     1680

ugacccccgu cgggcgccug aucaccgcga accccgugau caccgagagc acggagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgucg     1800

gggagaagaa gaucacccac cacuggcacc ggagcggcag cacccugggg aaggccuucu     1860

ccaccacgcu caagggcgcc cagcgccugg cggcccuggg ggacaccgcc ugggacuucg     1920

gcagcaucgg gggcguguuc aacuccaucg gccgggccgu ccaccaggug uucgggggcg     1980

ccuuccgcac ccucuucggg ggcaugagcu ggaucaccca ggggcugaug ggcgcgcugc     2040

uccuguggau gggggugaac gcccgggacc gcuccaucgc ccuggccuuc cucgcgacgg     2100

gcggcguccu gguguuccug gccaccaacg ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10375
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10375
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccau cacccgccgg ggguccgccu acuacaugua ccuggaccgc agcgacgccg      180

gcaaggccau cuccuucgcg accacgcugg gcgugaacaa gugccacgug cagaucaugg      240

accucgggca caugugcgac gccaccauga gcuacgagug cccgaugcug gacgagggcg      300

ucgagcccga cgacguggac ugcuggugca acaccaccuc cacgugggug gucuacggga      360

ccugccacca caagaagggc gaggcccggc gcagccggcg cgccgugacc cugcccuccc      420

acagcacccg gaagcuccag acgcgcuccc agaccuggcu ggagagccgc gaguacacca      480

agcaccugau caagguggag aacuggaucu uccggaaccc cggcuucgcc cucgucgcgg      540

uggccaucgc cuggcugcug ggguccagca ccucccagaa ggugaucuac cucgucauga      600

uccugcugau cgccccggcc uacagcaucc gcugcaucgg cguguccaac cgggacuucg      660

uggaggggau gagcggcggg acgugggucg acguggugcu cgagcacggc ggcugcguca      720

ccgugauggc gcaggacaag cccaccgugg acaucgagcu ggucaccacg accgugucca      780

acauggccga gguccgcagc uacugcuacg aggccagcau cuccgacaug gccagcgacu      840

cccggugccc cacccagggg gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagcgcac gcucguggac cggggcuggg ggaacggcug cgggcuguuc ggcaagggcu      960

cccuggucac gugcgcgaag uucacgugca gcaagaagau gaccgggaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcuga gcgugcacgg cucccagcac agcgggauga     1080

ucggcuacga gacggacgag gaccgggcca agguggaggu caccccgaac uccccgcgcg     1140

ccgaggccac ccugggcggg uucggcagcc ucgggcugga cugcgagccc cggaccggcc     1200

uggacuucuc cgaccucuac uaccugacga ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cucccguggc acgcgggggc cgacaccggc accccgcacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagcgc cagaccgucg     1380

uggugcuggg cagccaggag ggggcggucc acacggcccu ggccggcgcc cucgaggccg     1440

agauggacgg ggcgaagggc cggcuguucu ccgggcaccu gaagugccgc cucaagaugg     1500

acaagcugcg gcugaagggc gugagcuacu cccucugcac cgccgccuuc accuucacca     1560

aggugcccgc cgagacccug cacggcacgg ucaccgugga ggugcaguac gccgggaccg     1620

acggccccug caagaucccg guccagaugg cgguggacau gcagacccug acgcccgugg     1680

ggcgccucau caccgccaac cccgucauca ccgagagcac cgagaacucc aagaugaugc     1740

uggagcugga cccgccguuc ggcgacagcu acaucgugau cggggugggc gacaagaaga     1800

ucacgcacca cuggcaccgg agcggcagca cccuggggaa ggccuucucc accacgcuca     1860

agggcgccca gcgccuggcg gcccuggggg acaccgccug ggacuucggc agcaucgggg     1920

gcguguucaa cuccaucggc cgggccgucc accagguguu cgggggcgcc uuccgcaccc     1980

ucuucggggg caugagcugg aucacccagg ggcugauggg cgcgcugcuc cuguggaugg     2040

gggugaacgc ccgggaccgc uccaucgccc uggccuuccu cgcgacgggc ggcguccugg     2100

uguuccuggc caccaacgug cacgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10376
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10376
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgu cacccggcgc ggcuccgccu acuacaugua ccucgaccgg aacgacgccg      180

gggaggccau cagcuucccg accacgcugg gcaugaacaa gugcuacauc cagaucaugg      240

accuggggca caugugcgac gcgaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag cacguggguc guguacggca      360

cgugccacca caagaagggg gaggcccgcc ggucccgccg ggccgugacc cugcccagcc      420

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      480

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      540

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      600

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      660

uggaggggau guccggcggc acgugggugg acgucgugcu ggagcacggg ggcugcguga      720

ccgucauggc ccaggacaag cccaccgugg acaucgagcu cgugaccacg accgucagca      780

acauggccga ggugcgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      840

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgucu      900

gcaagcgcac gcugguggac cggggcuggg ggaacggcug cggccucuuc gggaagggca      960

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc     1020

cggagaaccu ggaguaccgc aucaugcucu ccguccacgg cagccagcac uccgggauga     1080

ucgugaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca     1140

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc     1200

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc     1260

ugguccacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1320

ggaccccgca cuggaacaac aaggaggccc ucguggaguu caaggacgcg cacgccaagc     1380

gccagaccgu ggucgugcug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1440

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1500

gccugaagau ggacaagcuc cggcugaagg gggucagcua cucccugugc accgccgccu     1560

ucaccuucac caagaucccc gcggagaccc uccacggcac ggugaccgug gagguccagu     1620

acgccgggac cgacggcccg ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacgcccgu cgggcgccug aucaccgcga accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgucg     1800

gggagaagaa gaucacgcac cacuggcacc ggagcggcag cacccugggg aaggccuucu     1860

ccaccacgcu caagggcgcc cagcgccugg cggcccuggg ggacaccgcc ugggacuucg     1920

gcagcaucgg gggcguguuc aacuccaucg gccgggccgu ccaccaggug uucgggggcg     1980

ccuuccgcac ccucuucggg ggcaugagcu ggaucaccca ggggcugaug ggcgcgcugc     2040

uccuguggau gggggugaac gcccgggacc gcuccaucgc ccuggccuuc cucgcgacgg     2100

gcggcguccu gguguuccug gccaccaacg ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10377
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10377
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga caucguccug gagcacgggg gcugcgugac cgugauggcc caggacaagc      720

ccaccgucga caucgagcuc gugaccacga ccgugagcaa cauggccgag guccgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgugug caagcgcacg cuggucgacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cgugcacggc agccagcacu ccgggaugau cgucaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgcgc acgccaagcg ccagaccgug gugguccugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggugagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gucaccgugg aggugcagua cgccgggacc gacggcccgu     1620

gcaagguccc cgcccagaug gccguggaca ugcagacccu gacgcccgug gggcgccuga     1680

ucaccgcgaa ccccgucauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcguggg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc aagggcgccc     1860

agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg ggcguguuca     1920

acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc cucuucgggg     1980

gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg     2040

cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10378
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10378
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac acgugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccacgagc accugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacgcgc aagcugcaga      420

cccggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac gagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

ccugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugacgacca ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacacgc aguacgucug caagcgcacc cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac acgggccacg     1080

agaccgacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggacgggc cuggacuuca     1200

gcgaccugua cuaccucacc augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagacggug gucgugcugg     1380

gcucccagga gggggccgug cacaccgccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacgcu ccacggcacc gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacgcu gacccccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca cggagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacccacc     1800

acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc aagggcgccc     1860

agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg ggcguguuca     1920

acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc cucuucgggg     1980

gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg     2040

cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10379
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10379
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcauc acccgccggg      120

gguccgccua cuacauguac cuggaccgca gcgacgccgg caaggccauc uccuucgcga      180

ccacgcuggg cgugaacaag ugccacgugc agaucaugga ccucgggcac augugcgacg      240

ccaccaugag cuacgagugc ccgaugcugg acgagggcgu cgagcccgac gacguggacu      300

gcuggugcaa caccaccucc acgugggugg ucuacgggac cugccaccac aagaagggcg      360

aggcccggcg cagccggcgc gccgugaccc ugcccuccca cagcacccgg aagcuccaga      420

cgcgcuccca gaccuggcug gagagccgcg aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu ccggaacccc ggcuucgccc ucgucgcggu ggccaucgcc uggcugcugg      540

gguccagcac cucccagaag gugaucuacc ucgucaugau ccugcugauc gccccggccu      600

acagcauccg cugcaucggc guguccaacc gggacuucgu ggaggggaug agcggcggga      660

cgugggucga cguggugcuc gagcacggcg gcugcgucac cgugauggcg caggacaagc      720

ccaccgugga caucgagcug gucaccacga ccguguccaa cauggccgag guccgcagcu      780

acugcuacga ggccagcauc uccgacaugg ccagcgacuc ccggugcccc acccaggggg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagcgcacg cucguggacc      900

ggggcugggg gaacggcugc gggcuguucg gcaagggcuc ccuggucacg ugcgcgaagu      960

ucacgugcag caagaagaug accgggaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcugag cgugcacggc ucccagcaca gcgggaugau cggcuacgag acggacgagg     1080

accgggccaa gguggagguc accccgaacu ccccgcgcgc cgaggccacc cugggcgggu     1140

ucggcagccu cgggcuggac ugcgagcccc ggaccggccu ggacuucucc gaccucuacu     1200

accugacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ucccguggca cgcgggggcc gacaccggca ccccgcacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagcgcc agaccgucgu ggugcugggc agccaggagg     1380

gggcggucca cacggcccug gccggcgccc ucgaggccga gauggacggg gcgaagggcc     1440

ggcuguucuc cgggcaccug aagugccgcc ucaagaugga caagcugcgg cugaagggcg     1500

ugagcuacuc ccucugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcacggu caccguggag gugcaguacg ccgggaccga cggccccugc aagaucccgg     1620

uccagauggc gguggacaug cagacccuga cgcccguggg gcgccucauc accgccaacc     1680

ccgucaucac cgagagcacc gagaacucca agaugaugcu ggagcuggac ccgccguucg     1740

gcgacagcua caucgugauc ggggugggcg acaagaagau cacgcaccac uggcaccgga     1800

gcggcagcac ccuggggaag gccuucucca ccacgcucaa gggcgcccag cgccuggcgg     1860

cccuggggga caccgccugg gacuucggca gcaucggggg cguguucaac uccaucggcc     1920

gggccgucca ccagguguuc gggggcgccu uccgcacccu cuucgggggc augagcugga     1980

ucacccaggg gcugaugggc gcgcugcucc uguggauggg ggugaacgcc cgggaccgcu     2040

ccaucgcccu ggccuuccuc gcgacgggcg gcguccuggu guuccuggcc accaacgugc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10380
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10380
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcguc acccggcgcg      120

gcuccgccua cuacauguac cucgaccgga acgacgccgg ggaggccauc agcuucccga      180

ccacgcuggg caugaacaag ugcuacaucc agaucaugga ccuggggcac augugcgacg      240

cgaccauguc cuacgagugc cccaugcucg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc acgugggucg uguacggcac gugccaccac aagaaggggg      360

aggcccgccg gucccgccgg gccgugaccc ugcccagcca cuccacccgc aagcugcaga      420

cgcggagcca gaccuggcuc gagucccgcg aguacaccaa gcaccugauc cgggucgaga      480

acuggaucuu ccgcaacccg ggcuucgccc uggccgcggc cgccaucgcc uggcuccugg      540

ggagcuccac cagccagaag gugaucuacc uggugaugau ccuccugauc gcccccgcgu      600

acuccauccg cugcaucggc gucagcaacc gggacuucgu ggaggggaug uccggcggca      660

cgugggugga cgucgugcug gagcacgggg gcugcgugac cgucauggcc caggacaagc      720

ccaccgugga caucgagcuc gugaccacga ccgucagcaa cauggccgag gugcgcagcu      780

acugcuacga ggccuccauc agcgacaugg ccuccgacag ccggugcccc acccaggggg      840

aggcguaccu ggacaagcag uccgacaccc aguacgucug caagcgcacg cugguggacc      900

ggggcugggg gaacggcugc ggccucuucg ggaagggcag ccuggugacc ugcgccaagu      960

ucgccugcuc caagaagaug accgggaaga gcauccagcc ggagaaccug gaguaccgca     1020

ucaugcucuc cguccacggc agccagcacu ccgggaugau cgugaacgac accggccacg     1080

agacggacga gaaccgggcc aagguggaga ucacccccaa cagcccccgc gcggaggcca     1140

cccugggcgg guucggcucc cuggggcucg acugcgagcc ccggaccggc cuggacuuca     1200

gcgaccugua cuaccucacg augaacaaca agcacuggcu gguccacaag gagugguucc     1260

acgacauccc gcugcccugg cacgccggcg ccgacaccgg gaccccgcac uggaacaaca     1320

aggaggcccu cguggaguuc aaggacgcgc acgccaagcg ccagaccgug gucgugcugg     1380

gcucccagga gggggccgug cacacggccc uggccggcgc gcuggaggcc gagauggacg     1440

gggccaaggg ccggcucagc uccggccacc ugaagugccg ccugaagaug gacaagcucc     1500

ggcugaaggg ggucagcuac ucccugugca ccgccgccuu caccuucacc aagauccccg     1560

cggagacccu ccacggcacg gugaccgugg agguccagua cgccgggacc gacggcccgu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacgcccguc gggcgccuga     1680

ucaccgcgaa ccccgugauc accgagagca ccgagaacag caagaugaug cucgagcugg     1740

acccgcccuu cggcgacucc uacaucguga ucggcgucgg ggagaagaag aucacgcacc     1800

acuggcaccg gagcggcagc acccugggga aggccuucuc caccacgcuc aagggcgccc     1860

agcgccuggc ggcccugggg gacaccgccu gggacuucgg cagcaucggg ggcguguuca     1920

acuccaucgg ccgggccguc caccaggugu ucgggggcgc cuuccgcacc cucuucgggg     1980

gcaugagcug gaucacccag gggcugaugg gcgcgcugcu ccuguggaug ggggugaacg     2040

cccgggaccg cuccaucgcc cuggccuucc ucgcgacggg cggcguccug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10381
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10381
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacg uggguggaca ucguccugga gcacgggggc ugcgugaccg      720

ugauggccca ggacaagccc accgucgaca ucgagcucgu gaccacgacc gugagcaaca      780

uggccgaggu ccgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgugugca      900

agcgcacgcu ggucgaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg ugcacggcag ccagcacucc gggaugaucg     1080

ucaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg ucgaguucaa ggacgcgcac gccaagcgcc     1380

agaccguggu gguccugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ugagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacccucc acggcacggu caccguggag gugcaguacg     1620

ccgggaccga cggcccgugc aagguccccg cccagauggc cguggacaug cagacccuga     1680

cgcccguggg gcgccugauc accgcgaacc ccgucaucac cgagagcacc gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcguggggg     1800

agaagaagau cacgcaccac uggcaccgga gcggcagcac ccuggggaag gccuucucca     1860

ccacgcucaa gggcgcccag cgccuggcgg cccuggggga caccgccugg gacuucggca     1920

gcaucggggg cguguucaac uccaucggcc gggccgucca ccagguguuc gggggcgccu     1980

uccgcacccu cuucgggggc augagcugga ucacccaggg gcugaugggc gcgcugcucc     2040

uguggauggg ggugaacgcc cgggaccgcu ccaucgcccu ggccuuccuc gcgacgggcg     2100

gcguccuggu guuccuggcc accaacgugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10382
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10382
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacac gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccacgagcac cugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacgcgcaa gcugcagacc cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacga gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacc uggguggacg ucgugcugga gcacgggggc ugcgugaccg      720

ucauggccca ggacaagccc accguggaca ucgagcucgu gacgaccacc gucagcaaca      780

uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacgcag uacgucugca      900

agcgcacccu gguggaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc gggaugaucg     1080

ugaacgacac gggccacgag accgacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggacgggccu ggacuucagc gaccuguacu accucaccau gaacaacaag cacuggcugg     1260

uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac gccaagcgcc     1380

agacgguggu cgugcugggc ucccaggagg gggccgugca caccgcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacgcucc acggcaccgu gaccguggag guccaguacg     1620

ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug cagacgcuga     1680

cccccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacg gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcgucgggg     1800

agaagaagau cacccaccac uggcaccgga gcggcagcac ccuggggaag gccuucucca     1860

ccacgcucaa gggcgcccag cgccuggcgg cccuggggga caccgccugg gacuucggca     1920

gcaucggggg cguguucaac uccaucggcc gggccgucca ccagguguuc gggggcgccu     1980

uccgcacccu cuucgggggc augagcugga ucacccaggg gcugaugggc gcgcugcucc     2040

uguggauggg ggugaacgcc cgggaccgcu ccaucgcccu ggccuuccuc gcgacgggcg     2100

gcguccuggu guuccuggcc accaacgugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10383
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10383
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccaucac ccgccggggg uccgccuacu acauguaccu ggaccgcagc gacgccggca      180

aggccaucuc cuucgcgacc acgcugggcg ugaacaagug ccacgugcag aucauggacc      240

ucgggcacau gugcgacgcc accaugagcu acgagugccc gaugcuggac gagggcgucg      300

agcccgacga cguggacugc uggugcaaca ccaccuccac gugggugguc uacgggaccu      360

gccaccacaa gaagggcgag gcccggcgca gccggcgcgc cgugacccug cccucccaca      420

gcacccggaa gcuccagacg cgcucccaga ccuggcugga gagccgcgag uacaccaagc      480

accugaucaa gguggagaac uggaucuucc ggaaccccgg cuucgcccuc gucgcggugg      540

ccaucgccug gcugcugggg uccagcaccu cccagaaggu gaucuaccuc gucaugaucc      600

ugcugaucgc cccggccuac agcauccgcu gcaucggcgu guccaaccgg gacuucgugg      660

aggggaugag cggcgggacg ugggucgacg uggugcucga gcacggcggc ugcgucaccg      720

ugauggcgca ggacaagccc accguggaca ucgagcuggu caccacgacc guguccaaca      780

uggccgaggu ccgcagcuac ugcuacgagg ccagcaucuc cgacauggcc agcgacuccc      840

ggugccccac ccagggggag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agcgcacgcu cguggaccgg ggcuggggga acggcugcgg gcuguucggc aagggcuccc      960

uggucacgug cgcgaaguuc acgugcagca agaagaugac cgggaagucc auccagcccg     1020

agaaccucga guaccgcauc augcugagcg ugcacggcuc ccagcacagc gggaugaucg     1080

gcuacgagac ggacgaggac cgggccaagg uggaggucac cccgaacucc ccgcgcgccg     1140

aggccacccu gggcggguuc ggcagccucg ggcuggacug cgagccccgg accggccugg     1200

acuucuccga ccucuacuac cugacgauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccccuc ccguggcacg cgggggccga caccggcacc ccgcacugga     1320

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagcgccag accgucgugg     1380

ugcugggcag ccaggagggg gcgguccaca cggcccuggc cggcgcccuc gaggccgaga     1440

uggacggggc gaagggccgg cuguucuccg ggcaccugaa gugccgccuc aagauggaca     1500

agcugcggcu gaagggcgug agcuacuccc ucugcaccgc cgccuucacc uucaccaagg     1560

ugcccgccga gacccugcac ggcacgguca ccguggaggu gcaguacgcc gggaccgacg     1620

gccccugcaa gaucccgguc cagauggcgg uggacaugca gacccugacg cccguggggc     1680

gccucaucac cgccaacccc gucaucaccg agagcaccga gaacuccaag augaugcugg     1740

agcuggaccc gccguucggc gacagcuaca ucgugaucgg ggugggcgac aagaagauca     1800

cgcaccacug gcaccggagc ggcagcaccc uggggaaggc cuucuccacc acgcucaagg     1860

gcgcccagcg ccuggcggcc cugggggaca ccgccuggga cuucggcagc aucgggggcg     1920

uguucaacuc caucggccgg gccguccacc agguguucgg gggcgccuuc cgcacccucu     1980

ucgggggcau gagcuggauc acccaggggc ugaugggcgc gcugcuccug uggauggggg     2040

ugaacgcccg ggaccgcucc aucgcccugg ccuuccucgc gacgggcggc guccuggugu     2100

uccuggccac caacgugcac gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10384
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10384
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgucac ccggcgcggc uccgccuacu acauguaccu cgaccggaac gacgccgggg      180

aggccaucag cuucccgacc acgcugggca ugaacaagug cuacauccag aucauggacc      240

uggggcacau gugcgacgcg accauguccu acgagugccc caugcucgac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac gugggucgug uacggcacgu      360

gccaccacaa gaagggggag gcccgccggu cccgccgggc cgugacccug cccagccacu      420

ccacccgcaa gcugcagacg cggagccaga ccuggcucga gucccgcgag uacaccaagc      480

accugauccg ggucgagaac uggaucuucc gcaacccggg cuucgcccug gccgcggccg      540

ccaucgccug gcuccugggg agcuccacca gccagaaggu gaucuaccug gugaugaucc      600

uccugaucgc ccccgcguac uccauccgcu gcaucggcgu cagcaaccgg gacuucgugg      660

aggggauguc cggcggcacg uggguggacg ucgugcugga gcacgggggc ugcgugaccg      720

ucauggccca ggacaagccc accguggaca ucgagcucgu gaccacgacc gucagcaaca      780

uggccgaggu gcgcagcuac ugcuacgagg ccuccaucag cgacauggcc uccgacagcc      840

ggugccccac ccagggggag gcguaccugg acaagcaguc cgacacccag uacgucugca      900

agcgcacgcu gguggaccgg ggcuggggga acggcugcgg ccucuucggg aagggcagcc      960

uggugaccug cgccaaguuc gccugcucca agaagaugac cgggaagagc auccagccgg     1020

agaaccugga guaccgcauc augcucuccg uccacggcag ccagcacucc gggaugaucg     1080

ugaacgacac cggccacgag acggacgaga accgggccaa gguggagauc acccccaaca     1140

gcccccgcgc ggaggccacc cugggcgggu ucggcucccu ggggcucgac ugcgagcccc     1200

ggaccggccu ggacuucagc gaccuguacu accucacgau gaacaacaag cacuggcugg     1260

uccacaagga gugguuccac gacaucccgc ugcccuggca cgccggcgcc gacaccggga     1320

ccccgcacug gaacaacaag gaggcccucg uggaguucaa ggacgcgcac gccaagcgcc     1380

agaccguggu cgugcugggc ucccaggagg gggccgugca cacggcccug gccggcgcgc     1440

uggaggccga gauggacggg gccaagggcc ggcucagcuc cggccaccug aagugccgcc     1500

ugaagaugga caagcuccgg cugaaggggg ucagcuacuc ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcg gagacccucc acggcacggu gaccguggag guccaguacg     1620

ccgggaccga cggcccgugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cgcccgucgg gcgccugauc accgcgaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu cgagcuggac ccgcccuucg gcgacuccua caucgugauc ggcgucgggg     1800

agaagaagau cacgcaccac uggcaccgga gcggcagcac ccuggggaag gccuucucca     1860

ccacgcucaa gggcgcccag cgccuggcgg cccuggggga caccgccugg gacuucggca     1920

gcaucggggg cguguucaac uccaucggcc gggccgucca ccagguguuc gggggcgccu     1980

uccgcacccu cuucgggggc augagcugga ucacccaggg gcugaugggc gcgcugcucc     2040

uguggauggg ggugaacgcc cgggaccgcu ccaucgcccu ggccuuccuc gcgacgggcg     2100

gcguccuggu guuccuggcc accaacgugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10385
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10385
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgugacc cugcccagcc      120

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      180

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      240

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      300

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      360

uggaggggau guccggcggc acgugggugg acaucguccu ggagcacggg ggcugcguga      420

ccgugauggc ccaggacaag cccaccgucg acaucgagcu cgugaccacg accgugagca      480

acauggccga gguccgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      540

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgugu      600

gcaagcgcac gcuggucgac cggggcuggg ggaacggcug cggccucuuc gggaagggca      660

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc      720

cggagaaccu ggaguaccgc aucaugcucu ccgugcacgg cagccagcac uccgggauga      780

ucgucaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca      840

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc      900

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc      960

uggugcacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1020

ggaccccgca cuggaacaac aaggaggccc ucgucgaguu caaggacgcg cacgccaagc     1080

gccagaccgu ggugguccug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1140

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1200

gccugaagau ggacaagcuc cggcugaagg gggugagcua cucccugugc accgccgccu     1260

ucaccuucac caagaucccc gcggagaccc uccacggcac ggucaccgug gaggugcagu     1320

acgccgggac cgacggcccg ugcaaggucc ccgcccagau ggccguggac augcagaccc     1380

ugacgcccgu ggggcgccug aucaccgcga accccgucau caccgagagc accgagaaca     1440

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgugg     1500

gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1560

aggccaccgu ccgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg     1620

gcagcguggg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1680

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacgcugc     1740

ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc cucgcccugg     1800

gcggcguccu gaucuuccuc uccaccgcgg ugagcgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10386
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10386
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgugacc cugcccagcc      120

acuccacgcg caagcugcag acccggagcc agaccuggcu cgagucccgc gaguacacca      180

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      240

ccgccaucgc cuggcuccug gggagcucca cgagccagaa ggugaucuac cuggugauga      300

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      360

uggaggggau guccggcggc accugggugg acgucgugcu ggagcacggg ggcugcguga      420

ccgucauggc ccaggacaag cccaccgugg acaucgagcu cgugacgacc accgucagca      480

acauggccga ggugcgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      540

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacg caguacgucu      600

gcaagcgcac ccugguggac cggggcuggg ggaacggcug cggccucuuc gggaagggca      660

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc      720

cggagaaccu ggaguaccgc aucaugcucu ccguccacgg cagccagcac uccgggauga      780

ucgugaacga cacgggccac gagaccgacg agaaccgggc caagguggag aucaccccca      840

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc      900

cccggacggg ccuggacuuc agcgaccugu acuaccucac caugaacaac aagcacuggc      960

ugguccacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1020

ggaccccgca cuggaacaac aaggaggccc ucguggaguu caaggacgcg cacgccaagc     1080

gccagacggu ggucgugcug ggcucccagg agggggccgu gcacaccgcc cuggccggcg     1140

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1200

gccugaagau ggacaagcuc cggcugaagg gggucagcua cucccugugc accgccgccu     1260

ucaccuucac caagaucccc gcggagacgc uccacggcac cgugaccgug gagguccagu     1320

acgccgggac cgacggcccg ugcaaggugc ccgcccagau ggccguggac augcagacgc     1380

ugacccccgu cgggcgccug aucaccgcga accccgugau caccgagagc acggagaaca     1440

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgucg     1500

gggagaagaa gaucacccac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1560

aggccaccgu gcgcggcgcc aagcggaugg ccgugcuggg ggacacggcg ugggacuucg     1620

gcagcgucgg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1680

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacccugc     1740

ugauguggcu cgggcugaac gccaagaacg gcuccaucag ccugaugugc cucgcgcugg     1800

gcggcgugcu gaucuuccuc uccaccgccg ugagcgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10387
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10387
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucgggau caucggccug cugcucacga ccgccauggc ggccgugacc cugcccuccc      120

acagcacccg gaagcuccag acgcgcuccc agaccuggcu ggagagccgc gaguacacca      180

agcaccugau caagguggag aacuggaucu uccggaaccc cggcuucgcc cucgucgcgg      240

uggccaucgc cuggcugcug ggguccagca ccucccagaa ggugaucuac cucgucauga      300

uccugcugau cgccccggcc uacagcaucc gcugcaucgg cguguccaac cgggacuucg      360

uggaggggau gagcggcggg acgugggucg acguggugcu cgagcacggc ggcugcguca      420

ccgugauggc gcaggacaag cccaccgugg acaucgagcu ggucaccacg accgugucca      480

acauggccga gguccgcagc uacugcuacg aggccagcau cuccgacaug gccagcgacu      540

cccggugccc cacccagggg gaggccuacc uggacaagca gagcgacacc caguacgugu      600

gcaagcgcac gcucguggac cggggcuggg ggaacggcug cgggcuguuc ggcaagggcu      660

cccuggucac gugcgcgaag uucacgugca gcaagaagau gaccgggaag uccauccagc      720

ccgagaaccu cgaguaccgc aucaugcuga gcgugcacgg cucccagcac agcgggauga      780

ucggcuacga gacggacgag gaccgggcca agguggaggu caccccgaac uccccgcgcg      840

ccgaggccac ccugggcggg uucggcagcc ucgggcugga cugcgagccc cggaccggcc      900

uggacuucuc cgaccucuac uaccugacga ugaacaacaa gcacuggcug gugcacaagg      960

agugguucca cgacaucccc cucccguggc acgcgggggc cgacaccggc accccgcacu     1020

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagcgc cagaccgucg     1080

uggugcuggg cagccaggag ggggcggucc acacggcccu ggccggcgcc cucgaggccg     1140

agauggacgg ggcgaagggc cggcuguucu ccgggcaccu gaagugccgc cucaagaugg     1200

acaagcugcg gcugaagggc gugagcuacu cccucugcac cgccgccuuc accuucacca     1260

aggugcccgc cgagacccug cacggcacgg ucaccgugga ggugcaguac gccgggaccg     1320

acggccccug caagaucccg guccagaugg cgguggacau gcagacccug acgcccgugg     1380

ggcgccucau caccgccaac cccgucauca ccgagagcac cgagaacucc aagaugaugc     1440

uggagcugga cccgccguuc ggcgacagcu acaucgugau cggggugggc gacaagaaga     1500

ucacgcacca cuggcaccgg agcggcucca ccaucgggaa ggccuucgag gccaccgucc     1560

gcggcgcgaa gcggauggcc gugcucgggg acaccgccug ggacuucggc agcgugggcg     1620

gcgucuucaa cucccugggg aaggggaucc accagaucuu cggcgccgcc uucaagagcc     1680

uguucggggg cauguccugg uucagccaga uccucaucgg gacgcugcug guguggcucg     1740

gccugaacac caagaacggc uccaucagcc ugaccugccu cgcgcugggc ggcgugauga     1800

ucuuccuguc caccgccguc agcgccugag gacuagugca ucacauuuaa aagcaucuca     1860

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     1920

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     1980

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2100

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2158


<210>  10388
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10388
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcguggggau cgugggccuc cugcugacga ccgccauggc ggccgugacc cugcccagcc      120

acuccacccg caagcugcag acgcggagcc agaccuggcu cgagucccgc gaguacacca      180

agcaccugau ccgggucgag aacuggaucu uccgcaaccc gggcuucgcc cuggccgcgg      240

ccgccaucgc cuggcuccug gggagcucca ccagccagaa ggugaucuac cuggugauga      300

uccuccugau cgcccccgcg uacuccaucc gcugcaucgg cgucagcaac cgggacuucg      360

uggaggggau guccggcggc acgugggugg acgucgugcu ggagcacggg ggcugcguga      420

ccgucauggc ccaggacaag cccaccgugg acaucgagcu cgugaccacg accgucagca      480

acauggccga ggugcgcagc uacugcuacg aggccuccau cagcgacaug gccuccgaca      540

gccggugccc cacccagggg gaggcguacc uggacaagca guccgacacc caguacgucu      600

gcaagcgcac gcugguggac cggggcuggg ggaacggcug cggccucuuc gggaagggca      660

gccuggugac cugcgccaag uucgccugcu ccaagaagau gaccgggaag agcauccagc      720

cggagaaccu ggaguaccgc aucaugcucu ccguccacgg cagccagcac uccgggauga      780

ucgugaacga caccggccac gagacggacg agaaccgggc caagguggag aucaccccca      840

acagcccccg cgcggaggcc acccugggcg gguucggcuc ccuggggcuc gacugcgagc      900

cccggaccgg ccuggacuuc agcgaccugu acuaccucac gaugaacaac aagcacuggc      960

ugguccacaa ggagugguuc cacgacaucc cgcugcccug gcacgccggc gccgacaccg     1020

ggaccccgca cuggaacaac aaggaggccc ucguggaguu caaggacgcg cacgccaagc     1080

gccagaccgu ggucgugcug ggcucccagg agggggccgu gcacacggcc cuggccggcg     1140

cgcuggaggc cgagauggac ggggccaagg gccggcucag cuccggccac cugaagugcc     1200

gccugaagau ggacaagcuc cggcugaagg gggucagcua cucccugugc accgccgccu     1260

ucaccuucac caagaucccc gcggagaccc uccacggcac ggugaccgug gagguccagu     1320

acgccgggac cgacggcccg ugcaaggugc ccgcccagau ggccguggac augcagaccc     1380

ugacgcccgu cgggcgccug aucaccgcga accccgugau caccgagagc accgagaaca     1440

gcaagaugau gcucgagcug gacccgcccu ucggcgacuc cuacaucgug aucggcgucg     1500

gggagaagaa gaucacgcac cacuggcacc ggagcggcuc caccaucggg aaggccuucg     1560

aggccaccgu gcgcggcgcc aagcggaugg ccgugcuggg ggacaccgcg ugggacuucg     1620

gcagcgucgg cggggcccuc aacucccugg gcaaggggau ccaccagauc uucggcgccg     1680

ccuucaagag ccuguucggg ggcauguccu gguucagcca gauccucauc ggcacgcugc     1740

ugauguggcu cgggcugaac accaagaacg gcuccaucag ccugaugugc cucgcccugg     1800

gcggcgugcu gaucuuccuc uccaccgcgg ugagcgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10389
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10389
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgc cgugacccug cccagccacu ccacccgcaa gcugcagacg cggagccaga      180

ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac uggaucuucc      240

gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg agcuccacca      300

gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac uccauccgcu      360

gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg uggguggaca      420

ucguccugga gcacgggggc ugcgugaccg ugauggccca ggacaagccc accgucgaca      480

ucgagcucgu gaccacgacc gugagcaaca uggccgaggu ccgcagcuac ugcuacgagg      540

ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag gcguaccugg      600

acaagcaguc cgacacccag uacgugugca agcgcacgcu ggucgaccgg ggcuggggga      660

acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc gccugcucca      720

agaagaugac cgggaagagc auccagccgg agaaccugga guaccgcauc augcucuccg      780

ugcacggcag ccagcacucc gggaugaucg ucaacgacac cggccacgag acggacgaga      840

accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc cugggcgggu      900

ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc gaccuguacu      960

accucacgau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacaucccgc     1020

ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag gaggcccucg     1080

ucgaguucaa ggacgcgcac gccaagcgcc agaccguggu gguccugggc ucccaggagg     1140

gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg gccaagggcc     1200

ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg cugaaggggg     1260

ugagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg gagacccucc     1320

acggcacggu caccguggag gugcaguacg ccgggaccga cggcccgugc aagguccccg     1380

cccagauggc cguggacaug cagacccuga cgcccguggg gcgccugauc accgcgaacc     1440

ccgucaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac ccgcccuucg     1500

gcgacuccua caucgugauc ggcguggggg agaagaagau cacgcaccac uggcaccgga     1560

gcggcuccac caucgggaag gccuucgagg ccaccguccg cggcgccaag cggauggccg     1620

ugcuggggga caccgcgugg gacuucggca gcgugggcgg ggcccucaac ucccugggca     1680

aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc auguccuggu     1740

ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc aagaacggcu     1800

ccaucagccu gaugugccuc gcccugggcg gcguccugau cuuccucucc accgcgguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10390
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10390
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgc cgugacccug cccagccacu ccacgcgcaa gcugcagacc cggagccaga      180

ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac uggaucuucc      240

gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg agcuccacga      300

gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac uccauccgcu      360

gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacc uggguggacg      420

ucgugcugga gcacgggggc ugcgugaccg ucauggccca ggacaagccc accguggaca      480

ucgagcucgu gacgaccacc gucagcaaca uggccgaggu gcgcagcuac ugcuacgagg      540

ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag gcguaccugg      600

acaagcaguc cgacacgcag uacgucugca agcgcacccu gguggaccgg ggcuggggga      660

acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc gccugcucca      720

agaagaugac cgggaagagc auccagccgg agaaccugga guaccgcauc augcucuccg      780

uccacggcag ccagcacucc gggaugaucg ugaacgacac gggccacgag accgacgaga      840

accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc cugggcgggu      900

ucggcucccu ggggcucgac ugcgagcccc ggacgggccu ggacuucagc gaccuguacu      960

accucaccau gaacaacaag cacuggcugg uccacaagga gugguuccac gacaucccgc     1020

ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag gaggcccucg     1080

uggaguucaa ggacgcgcac gccaagcgcc agacgguggu cgugcugggc ucccaggagg     1140

gggccgugca caccgcccug gccggcgcgc uggaggccga gauggacggg gccaagggcc     1200

ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg cugaaggggg     1260

ucagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg gagacgcucc     1320

acggcaccgu gaccguggag guccaguacg ccgggaccga cggcccgugc aaggugcccg     1380

cccagauggc cguggacaug cagacgcuga cccccgucgg gcgccugauc accgcgaacc     1440

ccgugaucac cgagagcacg gagaacagca agaugaugcu cgagcuggac ccgcccuucg     1500

gcgacuccua caucgugauc ggcgucgggg agaagaagau cacccaccac uggcaccgga     1560

gcggcuccac caucgggaag gccuucgagg ccaccgugcg cggcgccaag cggauggccg     1620

ugcuggggga cacggcgugg gacuucggca gcgucggcgg ggcccucaac ucccugggca     1680

aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc auguccuggu     1740

ucagccagau ccucaucggc acccugcuga uguggcucgg gcugaacgcc aagaacggcu     1800

ccaucagccu gaugugccuc gcgcugggcg gcgugcugau cuuccucucc accgccguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10391
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10391
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgc cgugacccug cccucccaca gcacccggaa gcuccagacg cgcucccaga      180

ccuggcugga gagccgcgag uacaccaagc accugaucaa gguggagaac uggaucuucc      240

ggaaccccgg cuucgcccuc gucgcggugg ccaucgccug gcugcugggg uccagcaccu      300

cccagaaggu gaucuaccuc gucaugaucc ugcugaucgc cccggccuac agcauccgcu      360

gcaucggcgu guccaaccgg gacuucgugg aggggaugag cggcgggacg ugggucgacg      420

uggugcucga gcacggcggc ugcgucaccg ugauggcgca ggacaagccc accguggaca      480

ucgagcuggu caccacgacc guguccaaca uggccgaggu ccgcagcuac ugcuacgagg      540

ccagcaucuc cgacauggcc agcgacuccc ggugccccac ccagggggag gccuaccugg      600

acaagcagag cgacacccag uacgugugca agcgcacgcu cguggaccgg ggcuggggga      660

acggcugcgg gcuguucggc aagggcuccc uggucacgug cgcgaaguuc acgugcagca      720

agaagaugac cgggaagucc auccagcccg agaaccucga guaccgcauc augcugagcg      780

ugcacggcuc ccagcacagc gggaugaucg gcuacgagac ggacgaggac cgggccaagg      840

uggaggucac cccgaacucc ccgcgcgccg aggccacccu gggcggguuc ggcagccucg      900

ggcuggacug cgagccccgg accggccugg acuucuccga ccucuacuac cugacgauga      960

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccuc ccguggcacg     1020

cgggggccga caccggcacc ccgcacugga acaacaagga ggcccuggug gaguucaagg     1080

acgcccacgc caagcgccag accgucgugg ugcugggcag ccaggagggg gcgguccaca     1140

cggcccuggc cggcgcccuc gaggccgaga uggacggggc gaagggccgg cuguucuccg     1200

ggcaccugaa gugccgccuc aagauggaca agcugcggcu gaagggcgug agcuacuccc     1260

ucugcaccgc cgccuucacc uucaccaagg ugcccgccga gacccugcac ggcacgguca     1320

ccguggaggu gcaguacgcc gggaccgacg gccccugcaa gaucccgguc cagauggcgg     1380

uggacaugca gacccugacg cccguggggc gccucaucac cgccaacccc gucaucaccg     1440

agagcaccga gaacuccaag augaugcugg agcuggaccc gccguucggc gacagcuaca     1500

ucgugaucgg ggugggcgac aagaagauca cgcaccacug gcaccggagc ggcuccacca     1560

ucgggaaggc cuucgaggcc accguccgcg gcgcgaagcg gauggccgug cucggggaca     1620

ccgccuggga cuucggcagc gugggcggcg ucuucaacuc ccuggggaag gggauccacc     1680

agaucuucgg cgccgccuuc aagagccugu ucgggggcau guccugguuc agccagaucc     1740

ucaucgggac gcugcuggug uggcucggcc ugaacaccaa gaacggcucc aucagccuga     1800

ccugccucgc gcugggcggc gugaugaucu uccuguccac cgccgucagc gccugaggac     1860

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     1920

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     1980

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2040

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2160

ggcucuuuuc agagccacca gaauu                                           2185


<210>  10392
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10392
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuca ucaucgccgu gcucaugucc gcgcaggaga      120

gcugggccgc cgugacccug cccagccacu ccacccgcaa gcugcagacg cggagccaga      180

ccuggcucga gucccgcgag uacaccaagc accugauccg ggucgagaac uggaucuucc      240

gcaacccggg cuucgcccug gccgcggccg ccaucgccug gcuccugggg agcuccacca      300

gccagaaggu gaucuaccug gugaugaucc uccugaucgc ccccgcguac uccauccgcu      360

gcaucggcgu cagcaaccgg gacuucgugg aggggauguc cggcggcacg uggguggacg      420

ucgugcugga gcacgggggc ugcgugaccg ucauggccca ggacaagccc accguggaca      480

ucgagcucgu gaccacgacc gucagcaaca uggccgaggu gcgcagcuac ugcuacgagg      540

ccuccaucag cgacauggcc uccgacagcc ggugccccac ccagggggag gcguaccugg      600

acaagcaguc cgacacccag uacgucugca agcgcacgcu gguggaccgg ggcuggggga      660

acggcugcgg ccucuucggg aagggcagcc uggugaccug cgccaaguuc gccugcucca      720

agaagaugac cgggaagagc auccagccgg agaaccugga guaccgcauc augcucuccg      780

uccacggcag ccagcacucc gggaugaucg ugaacgacac cggccacgag acggacgaga      840

accgggccaa gguggagauc acccccaaca gcccccgcgc ggaggccacc cugggcgggu      900

ucggcucccu ggggcucgac ugcgagcccc ggaccggccu ggacuucagc gaccuguacu      960

accucacgau gaacaacaag cacuggcugg uccacaagga gugguuccac gacaucccgc     1020

ugcccuggca cgccggcgcc gacaccggga ccccgcacug gaacaacaag gaggcccucg     1080

uggaguucaa ggacgcgcac gccaagcgcc agaccguggu cgugcugggc ucccaggagg     1140

gggccgugca cacggcccug gccggcgcgc uggaggccga gauggacggg gccaagggcc     1200

ggcucagcuc cggccaccug aagugccgcc ugaagaugga caagcuccgg cugaaggggg     1260

ucagcuacuc ccugugcacc gccgccuuca ccuucaccaa gauccccgcg gagacccucc     1320

acggcacggu gaccguggag guccaguacg ccgggaccga cggcccgugc aaggugcccg     1380

cccagauggc cguggacaug cagacccuga cgcccgucgg gcgccugauc accgcgaacc     1440

ccgugaucac cgagagcacc gagaacagca agaugaugcu cgagcuggac ccgcccuucg     1500

gcgacuccua caucgugauc ggcgucgggg agaagaagau cacgcaccac uggcaccgga     1560

gcggcuccac caucgggaag gccuucgagg ccaccgugcg cggcgccaag cggauggccg     1620

ugcuggggga caccgcgugg gacuucggca gcgucggcgg ggcccucaac ucccugggca     1680

aggggaucca ccagaucuuc ggcgccgccu ucaagagccu guucgggggc auguccuggu     1740

ucagccagau ccucaucggc acgcugcuga uguggcucgg gcugaacacc aagaacggcu     1800

ccaucagccu gaugugccuc gcccugggcg gcgugcugau cuuccucucc accgcgguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10393
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10393
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcgcc gugacccugc      120

ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag ucccgcgagu      180

acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc uucgcccugg      240

ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug aucuaccugg      300

ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc agcaaccggg      360

acuucgugga ggggaugucc ggcggcacgu ggguggacau cguccuggag cacgggggcu      420

gcgugaccgu gauggcccag gacaagccca ccgucgacau cgagcucgug accacgaccg      480

ugagcaacau ggccgagguc cgcagcuacu gcuacgaggc cuccaucagc gacauggccu      540

ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc gacacccagu      600

acgugugcaa gcgcacgcug gucgaccggg gcugggggaa cggcugcggc cucuucggga      660

agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc gggaagagca      720

uccagccgga gaaccuggag uaccgcauca ugcucuccgu gcacggcagc cagcacuccg      780

ggaugaucgu caacgacacc ggccacgaga cggacgagaa ccgggccaag guggagauca      840

cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug gggcucgacu      900

gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug aacaacaagc      960

acuggcuggu gcacaaggag ugguuccacg acaucccgcu gcccuggcac gccggcgccg     1020

acaccgggac cccgcacugg aacaacaagg aggcccucgu cgaguucaag gacgcgcacg     1080

ccaagcgcca gaccguggug guccugggcu cccaggaggg ggccgugcac acggcccugg     1140

ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc ggccaccuga     1200

agugccgccu gaagauggac aagcuccggc ugaagggggu gagcuacucc cugugcaccg     1260

ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacgguc accguggagg     1320

ugcaguacgc cgggaccgac ggcccgugca agguccccgc ccagauggcc guggacaugc     1380

agacccugac gcccgugggg cgccugauca ccgcgaaccc cgucaucacc gagagcaccg     1440

agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac aucgugaucg     1500

gcguggggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc aucgggaagg     1560

ccuucgaggc caccguccgc ggcgccaagc ggauggccgu gcugggggac accgcguggg     1620

acuucggcag cgugggcggg gcccucaacu cccugggcaa ggggauccac cagaucuucg     1680

gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc cucaucggca     1740

cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug augugccucg     1800

cccugggcgg cguccugauc uuccucucca ccgcggugag cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10394
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10394
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcgcc gugacccugc      120

ccagccacuc cacgcgcaag cugcagaccc ggagccagac cuggcucgag ucccgcgagu      180

acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc uucgcccugg      240

ccgcggccgc caucgccugg cuccugggga gcuccacgag ccagaaggug aucuaccugg      300

ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc agcaaccggg      360

acuucgugga ggggaugucc ggcggcaccu ggguggacgu cgugcuggag cacgggggcu      420

gcgugaccgu cauggcccag gacaagccca ccguggacau cgagcucgug acgaccaccg      480

ucagcaacau ggccgaggug cgcagcuacu gcuacgaggc cuccaucagc gacauggccu      540

ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc gacacgcagu      600

acgucugcaa gcgcacccug guggaccggg gcugggggaa cggcugcggc cucuucggga      660

agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc gggaagagca      720

uccagccgga gaaccuggag uaccgcauca ugcucuccgu ccacggcagc cagcacuccg      780

ggaugaucgu gaacgacacg ggccacgaga ccgacgagaa ccgggccaag guggagauca      840

cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug gggcucgacu      900

gcgagccccg gacgggccug gacuucagcg accuguacua ccucaccaug aacaacaagc      960

acuggcuggu ccacaaggag ugguuccacg acaucccgcu gcccuggcac gccggcgccg     1020

acaccgggac cccgcacugg aacaacaagg aggcccucgu ggaguucaag gacgcgcacg     1080

ccaagcgcca gacggugguc gugcugggcu cccaggaggg ggccgugcac accgcccugg     1140

ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc ggccaccuga     1200

agugccgccu gaagauggac aagcuccggc ugaagggggu cagcuacucc cugugcaccg     1260

ccgccuucac cuucaccaag auccccgcgg agacgcucca cggcaccgug accguggagg     1320

uccaguacgc cgggaccgac ggcccgugca aggugcccgc ccagauggcc guggacaugc     1380

agacgcugac ccccgucggg cgccugauca ccgcgaaccc cgugaucacc gagagcacgg     1440

agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac aucgugaucg     1500

gcgucgggga gaagaagauc acccaccacu ggcaccggag cggcuccacc aucgggaagg     1560

ccuucgaggc caccgugcgc ggcgccaagc ggauggccgu gcugggggac acggcguggg     1620

acuucggcag cgucggcggg gcccucaacu cccugggcaa ggggauccac cagaucuucg     1680

gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc cucaucggca     1740

cccugcugau guggcucggg cugaacgcca agaacggcuc caucagccug augugccucg     1800

cgcugggcgg cgugcugauc uuccucucca ccgccgugag cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10395
<211>  2164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10395
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcgcc gugacccugc      120

ccucccacag cacccggaag cuccagacgc gcucccagac cuggcuggag agccgcgagu      180

acaccaagca ccugaucaag guggagaacu ggaucuuccg gaaccccggc uucgcccucg      240

ucgcgguggc caucgccugg cugcuggggu ccagcaccuc ccagaaggug aucuaccucg      300

ucaugauccu gcugaucgcc ccggccuaca gcauccgcug caucggcgug uccaaccggg      360

acuucgugga ggggaugagc ggcgggacgu gggucgacgu ggugcucgag cacggcggcu      420

gcgucaccgu gauggcgcag gacaagccca ccguggacau cgagcugguc accacgaccg      480

uguccaacau ggccgagguc cgcagcuacu gcuacgaggc cagcaucucc gacauggcca      540

gcgacucccg gugccccacc cagggggagg ccuaccugga caagcagagc gacacccagu      600

acgugugcaa gcgcacgcuc guggaccggg gcugggggaa cggcugcggg cuguucggca      660

agggcucccu ggucacgugc gcgaaguuca cgugcagcaa gaagaugacc gggaagucca      720

uccagcccga gaaccucgag uaccgcauca ugcugagcgu gcacggcucc cagcacagcg      780

ggaugaucgg cuacgagacg gacgaggacc gggccaaggu ggaggucacc ccgaacuccc      840

cgcgcgccga ggccacccug ggcggguucg gcagccucgg gcuggacugc gagccccgga      900

ccggccugga cuucuccgac cucuacuacc ugacgaugaa caacaagcac uggcuggugc      960

acaaggagug guuccacgac aucccccucc cguggcacgc gggggccgac accggcaccc     1020

cgcacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc aagcgccaga     1080

ccgucguggu gcugggcagc caggaggggg cgguccacac ggcccuggcc ggcgcccucg     1140

aggccgagau ggacggggcg aagggccggc uguucuccgg gcaccugaag ugccgccuca     1200

agauggacaa gcugcggcug aagggcguga gcuacucccu cugcaccgcc gccuucaccu     1260

ucaccaaggu gcccgccgag acccugcacg gcacggucac cguggaggug caguacgccg     1320

ggaccgacgg ccccugcaag aucccggucc agauggcggu ggacaugcag acccugacgc     1380

ccguggggcg ccucaucacc gccaaccccg ucaucaccga gagcaccgag aacuccaaga     1440

ugaugcugga gcuggacccg ccguucggcg acagcuacau cgugaucggg gugggcgaca     1500

agaagaucac gcaccacugg caccggagcg gcuccaccau cgggaaggcc uucgaggcca     1560

ccguccgcgg cgcgaagcgg auggccgugc ucggggacac cgccugggac uucggcagcg     1620

ugggcggcgu cuucaacucc cuggggaagg ggauccacca gaucuucggc gccgccuuca     1680

agagccuguu cgggggcaug uccugguuca gccagauccu caucgggacg cugcuggugu     1740

ggcucggccu gaacaccaag aacggcucca ucagccugac cugccucgcg cugggcggcg     1800

ugaugaucuu ccuguccacc gccgucagcg ccugaggacu agugcaucac auuuaaaagc     1860

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     1920

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     1980

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2160

aauu                                                                  2164


<210>  10396
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10396
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgccg cgacccgcgu ccacagcgcc gugacccugc      120

ccagccacuc cacccgcaag cugcagacgc ggagccagac cuggcucgag ucccgcgagu      180

acaccaagca ccugauccgg gucgagaacu ggaucuuccg caacccgggc uucgcccugg      240

ccgcggccgc caucgccugg cuccugggga gcuccaccag ccagaaggug aucuaccugg      300

ugaugauccu ccugaucgcc cccgcguacu ccauccgcug caucggcguc agcaaccggg      360

acuucgugga ggggaugucc ggcggcacgu ggguggacgu cgugcuggag cacgggggcu      420

gcgugaccgu cauggcccag gacaagccca ccguggacau cgagcucgug accacgaccg      480

ucagcaacau ggccgaggug cgcagcuacu gcuacgaggc cuccaucagc gacauggccu      540

ccgacagccg gugccccacc cagggggagg cguaccugga caagcagucc gacacccagu      600

acgucugcaa gcgcacgcug guggaccggg gcugggggaa cggcugcggc cucuucggga      660

agggcagccu ggugaccugc gccaaguucg ccugcuccaa gaagaugacc gggaagagca      720

uccagccgga gaaccuggag uaccgcauca ugcucuccgu ccacggcagc cagcacuccg      780

ggaugaucgu gaacgacacc ggccacgaga cggacgagaa ccgggccaag guggagauca      840

cccccaacag cccccgcgcg gaggccaccc ugggcggguu cggcucccug gggcucgacu      900

gcgagccccg gaccggccug gacuucagcg accuguacua ccucacgaug aacaacaagc      960

acuggcuggu ccacaaggag ugguuccacg acaucccgcu gcccuggcac gccggcgccg     1020

acaccgggac cccgcacugg aacaacaagg aggcccucgu ggaguucaag gacgcgcacg     1080

ccaagcgcca gaccgugguc gugcugggcu cccaggaggg ggccgugcac acggcccugg     1140

ccggcgcgcu ggaggccgag auggacgggg ccaagggccg gcucagcucc ggccaccuga     1200

agugccgccu gaagauggac aagcuccggc ugaagggggu cagcuacucc cugugcaccg     1260

ccgccuucac cuucaccaag auccccgcgg agacccucca cggcacggug accguggagg     1320

uccaguacgc cgggaccgac ggcccgugca aggugcccgc ccagauggcc guggacaugc     1380

agacccugac gcccgucggg cgccugauca ccgcgaaccc cgugaucacc gagagcaccg     1440

agaacagcaa gaugaugcuc gagcuggacc cgcccuucgg cgacuccuac aucgugaucg     1500

gcgucgggga gaagaagauc acgcaccacu ggcaccggag cggcuccacc aucgggaagg     1560

ccuucgaggc caccgugcgc ggcgccaagc ggauggccgu gcugggggac accgcguggg     1620

acuucggcag cgucggcggg gcccucaacu cccugggcaa ggggauccac cagaucuucg     1680

gcgccgccuu caagagccug uucgggggca uguccugguu cagccagauc cucaucggca     1740

cgcugcugau guggcucggg cugaacacca agaacggcuc caucagccug augugccucg     1800

cccugggcgg cgugcugauc uuccucucca ccgcggugag cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10397
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10397
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      180

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      240

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      300

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      360

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacaucg      420

uccuggagca cgggggcugc gugaccguga uggcccagga caagcccacc gucgacaucg      480

agcucgugac cacgaccgug agcaacaugg ccgagguccg cagcuacugc uacgaggccu      540

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      600

agcaguccga cacccaguac gugugcaagc gcacgcuggu cgaccggggc ugggggaacg      660

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      720

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgugc      780

acggcagcca gcacuccggg augaucguca acgacaccgg ccacgagacg gacgagaacc      840

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      900

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      960

ucacgaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccgcugc     1020

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgucg     1080

aguucaagga cgcgcacgcc aagcgccaga ccgugguggu ccugggcucc caggaggggg     1140

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1200

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguga     1260

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1320

gcacggucac cguggaggug caguacgccg ggaccgacgg cccgugcaag guccccgccc     1380

agauggccgu ggacaugcag acccugacgc ccguggggcg ccugaucacc gcgaaccccg     1440

ucaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1500

acuccuacau cgugaucggc gugggggaga agaagaucac gcaccacugg caccggagcg     1560

gcuccaccau cgggaaggcc uucgaggcca ccguccgcgg cgccaagcgg auggccgugc     1620

ugggggacac cgcgugggac uucggcagcg ugggcggggc ccucaacucc cugggcaagg     1680

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     1740

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     1800

ucagccugau gugccucgcc cugggcggcg uccugaucuu ccucuccacc gcggugagcg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10398
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10398
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgccgu gacccugccc agccacucca cgcgcaagcu gcagacccgg agccagaccu      180

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      240

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccacgagcc      300

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      360

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcaccugg guggacgucg      420

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      480

agcucgugac gaccaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      540

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      600

agcaguccga cacgcaguac gucugcaagc gcacccuggu ggaccggggc ugggggaacg      660

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      720

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc      780

acggcagcca gcacuccggg augaucguga acgacacggg ccacgagacc gacgagaacc      840

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      900

gcucccuggg gcucgacugc gagccccgga cgggccugga cuucagcgac cuguacuacc      960

ucaccaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc     1020

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg     1080

aguucaagga cgcgcacgcc aagcgccaga cgguggucgu gcugggcucc caggaggggg     1140

ccgugcacac cgcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1200

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1260

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acgcuccacg     1320

gcaccgugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1380

agauggccgu ggacaugcag acgcugaccc ccgucgggcg ccugaucacc gcgaaccccg     1440

ugaucaccga gagcacggag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1500

acuccuacau cgugaucggc gucggggaga agaagaucac ccaccacugg caccggagcg     1560

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1620

ugggggacac ggcgugggac uucggcagcg ucggcggggc ccucaacucc cugggcaagg     1680

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     1740

gccagauccu caucggcacc cugcugaugu ggcucgggcu gaacgccaag aacggcucca     1800

ucagccugau gugccucgcg cugggcggcg ugcugaucuu ccucuccacc gccgugagcg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10399
<211>  2182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10399
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgccgu gacccugccc ucccacagca cccggaagcu ccagacgcgc ucccagaccu      180

ggcuggagag ccgcgaguac accaagcacc ugaucaaggu ggagaacugg aucuuccgga      240

accccggcuu cgcccucguc gcgguggcca ucgccuggcu gcuggggucc agcaccuccc      300

agaaggugau cuaccucguc augauccugc ugaucgcccc ggccuacagc auccgcugca      360

ucggcguguc caaccgggac uucguggagg ggaugagcgg cgggacgugg gucgacgugg      420

ugcucgagca cggcggcugc gucaccguga uggcgcagga caagcccacc guggacaucg      480

agcuggucac cacgaccgug uccaacaugg ccgagguccg cagcuacugc uacgaggcca      540

gcaucuccga cauggccagc gacucccggu gccccaccca gggggaggcc uaccuggaca      600

agcagagcga cacccaguac gugugcaagc gcacgcucgu ggaccggggc ugggggaacg      660

gcugcgggcu guucggcaag ggcucccugg ucacgugcgc gaaguucacg ugcagcaaga      720

agaugaccgg gaaguccauc cagcccgaga accucgagua ccgcaucaug cugagcgugc      780

acggcuccca gcacagcggg augaucggcu acgagacgga cgaggaccgg gccaaggugg      840

aggucacccc gaacuccccg cgcgccgagg ccacccuggg cggguucggc agccucgggc      900

uggacugcga gccccggacc ggccuggacu ucuccgaccu cuacuaccug acgaugaaca      960

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccucccg uggcacgcgg     1020

gggccgacac cggcaccccg cacuggaaca acaaggaggc ccugguggag uucaaggacg     1080

cccacgccaa gcgccagacc gucguggugc ugggcagcca ggagggggcg guccacacgg     1140

cccuggccgg cgcccucgag gccgagaugg acggggcgaa gggccggcug uucuccgggc     1200

accugaagug ccgccucaag auggacaagc ugcggcugaa gggcgugagc uacucccucu     1260

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc acggucaccg     1320

uggaggugca guacgccggg accgacggcc ccugcaagau cccgguccag auggcggugg     1380

acaugcagac ccugacgccc guggggcgcc ucaucaccgc caaccccguc aucaccgaga     1440

gcaccgagaa cuccaagaug augcuggagc uggacccgcc guucggcgac agcuacaucg     1500

ugaucggggu gggcgacaag aagaucacgc accacuggca ccggagcggc uccaccaucg     1560

ggaaggccuu cgaggccacc guccgcggcg cgaagcggau ggccgugcuc ggggacaccg     1620

ccugggacuu cggcagcgug ggcggcgucu ucaacucccu ggggaagggg auccaccaga     1680

ucuucggcgc cgccuucaag agccuguucg ggggcauguc cugguucagc cagauccuca     1740

ucgggacgcu gcuggugugg cucggccuga acaccaagaa cggcuccauc agccugaccu     1800

gccucgcgcu gggcggcgug augaucuucc uguccaccgc cgucagcgcc ugaggacuag     1860

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     1920

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     1980

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2040

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2160

ucuuuucaga gccaccagaa uu                                              2182


<210>  10400
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10400
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcagcgc cggguccauc auguggcugg ccagccucgc gguggucauc gccugcgccg      120

gcgccgccgu gacccugccc agccacucca cccgcaagcu gcagacgcgg agccagaccu      180

ggcucgaguc ccgcgaguac accaagcacc ugauccgggu cgagaacugg aucuuccgca      240

acccgggcuu cgcccuggcc gcggccgcca ucgccuggcu ccuggggagc uccaccagcc      300

agaaggugau cuaccuggug augauccucc ugaucgcccc cgcguacucc auccgcugca      360

ucggcgucag caaccgggac uucguggagg ggauguccgg cggcacgugg guggacgucg      420

ugcuggagca cgggggcugc gugaccguca uggcccagga caagcccacc guggacaucg      480

agcucgugac cacgaccguc agcaacaugg ccgaggugcg cagcuacugc uacgaggccu      540

ccaucagcga cauggccucc gacagccggu gccccaccca gggggaggcg uaccuggaca      600

agcaguccga cacccaguac gucugcaagc gcacgcuggu ggaccggggc ugggggaacg      660

gcugcggccu cuucgggaag ggcagccugg ugaccugcgc caaguucgcc ugcuccaaga      720

agaugaccgg gaagagcauc cagccggaga accuggagua ccgcaucaug cucuccgucc      780

acggcagcca gcacuccggg augaucguga acgacaccgg ccacgagacg gacgagaacc      840

gggccaaggu ggagaucacc cccaacagcc cccgcgcgga ggccacccug ggcggguucg      900

gcucccuggg gcucgacugc gagccccgga ccggccugga cuucagcgac cuguacuacc      960

ucacgaugaa caacaagcac uggcuggucc acaaggagug guuccacgac aucccgcugc     1020

ccuggcacgc cggcgccgac accgggaccc cgcacuggaa caacaaggag gcccucgugg     1080

aguucaagga cgcgcacgcc aagcgccaga ccguggucgu gcugggcucc caggaggggg     1140

ccgugcacac ggcccuggcc ggcgcgcugg aggccgagau ggacggggcc aagggccggc     1200

ucagcuccgg ccaccugaag ugccgccuga agauggacaa gcuccggcug aaggggguca     1260

gcuacucccu gugcaccgcc gccuucaccu ucaccaagau ccccgcggag acccuccacg     1320

gcacggugac cguggagguc caguacgccg ggaccgacgg cccgugcaag gugcccgccc     1380

agauggccgu ggacaugcag acccugacgc ccgucgggcg ccugaucacc gcgaaccccg     1440

ugaucaccga gagcaccgag aacagcaaga ugaugcucga gcuggacccg cccuucggcg     1500

acuccuacau cgugaucggc gucggggaga agaagaucac gcaccacugg caccggagcg     1560

gcuccaccau cgggaaggcc uucgaggcca ccgugcgcgg cgccaagcgg auggccgugc     1620

ugggggacac cgcgugggac uucggcagcg ucggcggggc ccucaacucc cugggcaagg     1680

ggauccacca gaucuucggc gccgccuuca agagccuguu cgggggcaug uccugguuca     1740

gccagauccu caucggcacg cugcugaugu ggcucgggcu gaacaccaag aacggcucca     1800

ucagccugau gugccucgcc cugggcggcg ugcugaucuu ccucuccacc gcggugagcg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10401
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10401
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1920

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     2040

ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc cucgcccucg     2100

gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10402
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10402
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caccugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1920

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     2040

ucauguggcu cggccucaac gccaagaacg gcuccaucuc ccucaugugc cucgcccucg     2100

gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10403
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10403
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccau cacccgccgc ggcuccgccu acuacaugua ccucgaccgc uccgacgccg      180

gcaaggccau cuccuucgcc accacccucg gcgucaacaa gugccacguc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau caaggucgag aacuggaucu uccgcaaccc cggcuucgcc cucgucgccg      540

ucgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucaccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucggcuacga gaccgacgag gaccgcgcca aggucgaggu cacccccaac uccccccgcg     1140

ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc cgcaccggcc     1200

ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc guccacaagg     1260

agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc cagaccgucg     1380

ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg     1440

agauggacgg cgccaagggc cgccucuucu ccggccaccu caagugccgc cucaagaugg     1500

acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc accuucacca     1560

agguccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac gccggcaccg     1620

acggccccug caagaucccc guccagaugg ccgucgacau gcagacccuc acccccgucg     1680

gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc aagaugaugc     1740

ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc gacaagaaga     1800

ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag gccaccgucc     1860

gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc uccgucggcg     1920

gcgucuucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc uucaaguccc     1980

ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc gucuggcucg     2040

gccucaacac caagaacggc uccaucuccc ucaccugccu cgcccucggc ggcgucauga     2100

ucuuccucuc caccgccguc uccgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10404
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10404
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1860

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1920

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     2040

ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc cucgcccucg     2100

gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10405
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10405
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10406
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10406
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac accugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaacg     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10407
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10407
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cggcuacgag accgacgagg     1080

accgcgccaa ggucgagguc acccccaacu ccccccgcgc cgaggccacc cucggcggcu     1140

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu     1200

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1260

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1320

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1380

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1440

gccucuucuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1500

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gguccccgcc gagacccucc     1560

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagauccccg     1620

uccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1680

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1740

gcgacuccua caucgucauc ggcgucggcg acaagaagau cacccaccac uggcaccgcu     1800

ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag cgcauggccg     1860

uccucggcga caccgccugg gacuucggcu ccgucggcgg cgucuucaac ucccucggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc auguccuggu     1980

ucucccagau ccucaucggc acccuccucg ucuggcucgg ccucaacacc aagaacggcu     2040

ccaucucccu caccugccuc gcccucggcg gcgucaugau cuuccucucc accgccgucu     2100

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10408
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10408
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc accaucggca aggccuucga ggccaccguc cgcggcgcca     1860

agcgcauggc cguccucggc gacaccgccu gggacuucgg cuccgucggc ggcgcccuca     1920

acucccucgg caagggcauc caccagaucu ucggcgccgc cuucaagucc cucuucggcg     1980

gcauguccug guucucccag auccucaucg gcacccuccu cauguggcuc ggccucaaca     2040

ccaagaacgg cuccaucucc cucaugugcc ucgcccucgg cggcguccuc aucuuccucu     2100

ccaccgccgu cuccgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10409
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10409
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag gccuucgagg     1860

ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg gacuucggcu     1920

ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc acccuccuca     2040

uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc gcccucggcg     2100

gcguccucau cuuccucucc accgccgucu ccgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10410
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10410
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacac cugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag gccuucgagg     1860

ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg gacuucggcu     1920

ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc acccuccuca     2040

uguggcucgg ccucaacgcc aagaacggcu ccaucucccu caugugccuc gcccucggcg     2100

gcguccucau cuuccucucc accgccgucu ccgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10411
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10411
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccaucac ccgccgcggc uccgccuacu acauguaccu cgaccgcucc gacgccggca      180

aggccaucuc cuucgccacc acccucggcg ucaacaagug ccacguccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucaucaa ggucgagaac uggaucuucc gcaaccccgg cuucgcccuc gucgccgucg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc accugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

gcuacgagac cgacgaggac cgcgccaagg ucgaggucac ccccaacucc ccccgcgccg     1140

aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc accggccucg     1200

acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc cacaaggagu     1260

gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag accgucgucg     1380

uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc gaggccgaga     1440

uggacggcgc caagggccgc cucuucuccg gccaccucaa gugccgccuc aagauggaca     1500

agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc uucaccaagg     1560

uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg     1620

gccccugcaa gauccccguc cagauggccg ucgacaugca gacccucacc cccgucggcc     1680

gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag augaugcucg     1740

agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgac aagaagauca     1800

cccaccacug gcaccgcucc ggcuccacca ucggcaaggc cuucgaggcc accguccgcg     1860

gcgccaagcg cauggccguc cucggcgaca ccgccuggga cuucggcucc gucggcggcg     1920

ucuucaacuc ccucggcaag ggcauccacc agaucuucgg cgccgccuuc aagucccucu     1980

ucggcggcau guccugguuc ucccagaucc ucaucggcac ccuccucguc uggcucggcc     2040

ucaacaccaa gaacggcucc aucucccuca ccugccucgc ccucggcggc gucaugaucu     2100

uccucuccac cgccgucucc gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10412
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10412
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac caucggcaag gccuucgagg     1860

ccaccguccg cggcgccaag cgcauggccg uccucggcga caccgccugg gacuucggcu     1920

ccgucggcgg cgcccucaac ucccucggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagucccu cuucggcggc auguccuggu ucucccagau ccucaucggc acccuccuca     2040

uguggcucgg ccucaacacc aagaacggcu ccaucucccu caugugccuc gcccucggcg     2100

gcguccucau cuuccucucc accgccgucu ccgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10413
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10413
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc cacccucggc aaggccuucu     1860

ccaccacccu caagggcgcc cagcgccucg ccgcccucgg cgacaccgcc ugggacuucg     1920

gcuccaucgg cggcgucuuc aacuccaucg gccgcgccgu ccaccagguc uucggcggcg     1980

ccuuccgcac ccucuucggc ggcauguccu ggaucaccca gggccucaug ggcgcccucc     2040

uccucuggau gggcgucaac gcccgcgacc gcuccaucgc ccucgccuuc cucgccaccg     2100

gcggcguccu cgucuuccuc gccaccaacg uccacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10414
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10414
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caccugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc cacccucggc aaggccuucu     1860

ccaccacccu caagggcgcc cagcgccucg ccgcccucgg cgacaccgcc ugggacuucg     1920

gcuccaucgg cggcgucuuc aacuccaucg gccgcgccgu ccaccagguc uucggcggcg     1980

ccuuccgcac ccucuucggc ggcauguccu ggaucaccca gggccucaug ggcgcccucc     2040

uccucuggau gggcgucaac gcccgcgacc gcuccaucgc ccucgccuuc cucgccaccg     2100

gcggcguccu cgucuuccuc gccaccaacg uccacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10415
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10415
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccau cacccgccgc ggcuccgccu acuacaugua ccucgaccgc uccgacgccg      180

gcaaggccau cuccuucgcc accacccucg gcgucaacaa gugccacguc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau caaggucgag aacuggaucu uccgcaaccc cggcuucgcc cucgucgccg      540

ucgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucaccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucggcuacga gaccgacgag gaccgcgcca aggucgaggu cacccccaac uccccccgcg     1140

ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc cgcaccggcc     1200

ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc guccacaagg     1260

agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc cagaccgucg     1380

ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg     1440

agauggacgg cgccaagggc cgccucuucu ccggccaccu caagugccgc cucaagaugg     1500

acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc accuucacca     1560

agguccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac gccggcaccg     1620

acggccccug caagaucccc guccagaugg ccgucgacau gcagacccuc acccccgucg     1680

gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc aagaugaugc     1740

ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc gacaagaaga     1800

ucacccacca cuggcaccgc uccggcucca cccucggcaa ggccuucucc accacccuca     1860

agggcgccca gcgccucgcc gcccucggcg acaccgccug ggacuucggc uccaucggcg     1920

gcgucuucaa cuccaucggc cgcgccgucc accaggucuu cggcggcgcc uuccgcaccc     1980

ucuucggcgg cauguccugg aucacccagg gccucauggg cgcccuccuc cucuggaugg     2040

gcgucaacgc ccgcgaccgc uccaucgccc ucgccuuccu cgccaccggc ggcguccucg     2100

ucuuccucgc caccaacguc cacgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10416
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10416
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgu cacccgccgc ggcuccgccu acuacaugua ccucgaccgc aacgacgccg      180

gcgaggccau cuccuucccc accacccucg gcaugaacaa gugcuacauc cagaucaugg      240

accucggcca caugugcgac gccaccaugu ccuacgagug ccccaugcuc gacgagggcg      300

ucgagcccga cgacgucgac ugcuggugca acaccaccuc caccuggguc gucuacggca      360

ccugccacca caagaagggc gaggcccgcc gcucccgccg cgccgucacc cuccccuccc      420

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      480

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      540

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      600

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      660

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      720

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      780

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      840

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      900

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      960

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc     1020

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga     1080

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca     1140

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc     1200

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc     1260

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1380

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1440

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1500

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1620

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1680

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1740

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1800

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc cacccucggc aaggccuucu     1860

ccaccacccu caagggcgcc cagcgccucg ccgcccucgg cgacaccgcc ugggacuucg     1920

gcuccaucgg cggcgucuuc aacuccaucg gccgcgccgu ccaccagguc uucggcggcg     1980

ccuuccgcac ccucuucggc ggcauguccu ggaucaccca gggccucaug ggcgcccucc     2040

uccucuggau gggcgucaac gcccgcgacc gcuccaucgc ccucgccuuc cucgccaccg     2100

gcggcguccu cgucuuccuc gccaccaacg uccacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10417
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10417
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga caucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc aagggcgccc     1860

agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc ggcgucuuca     1920

acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc cucuucggcg     1980

gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg     2040

cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc gucuuccucg     2100

ccaccaacgu ccacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10418
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10418
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac accugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc aagggcgccc     1860

agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc ggcgucuuca     1920

acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc cucuucggcg     1980

gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg     2040

cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc gucuuccucg     2100

ccaccaacgu ccacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10419
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10419
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccauc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgcu ccgacgccgg caaggccauc uccuucgcca      180

ccacccucgg cgucaacaag ugccacgucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc aaggucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgucgccgu cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucaccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cggcuacgag accgacgagg     1080

accgcgccaa ggucgagguc acccccaacu ccccccgcgc cgaggccacc cucggcggcu     1140

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu     1200

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1260

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1320

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1380

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1440

gccucuucuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1500

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gguccccgcc gagacccucc     1560

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagauccccg     1620

uccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1680

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1740

gcgacuccua caucgucauc ggcgucggcg acaagaagau cacccaccac uggcaccgcu     1800

ccggcuccac ccucggcaag gccuucucca ccacccucaa gggcgcccag cgccucgccg     1860

cccucggcga caccgccugg gacuucggcu ccaucggcgg cgucuucaac uccaucggcc     1920

gcgccgucca ccaggucuuc ggcggcgccu uccgcacccu cuucggcggc auguccugga     1980

ucacccaggg ccucaugggc gcccuccucc ucuggauggg cgucaacgcc cgcgaccgcu     2040

ccaucgcccu cgccuuccuc gccaccggcg gcguccucgu cuuccucgcc accaacgucc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10420
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10420
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccguc acccgccgcg      120

gcuccgccua cuacauguac cucgaccgca acgacgccgg cgaggccauc uccuucccca      180

ccacccucgg caugaacaag ugcuacaucc agaucaugga ccucggccac augugcgacg      240

ccaccauguc cuacgagugc cccaugcucg acgagggcgu cgagcccgac gacgucgacu      300

gcuggugcaa caccaccucc accugggucg ucuacggcac cugccaccac aagaagggcg      360

aggcccgccg cucccgccgc gccgucaccc uccccuccca cuccacccgc aagcuccaga      420

cccgcuccca gaccuggcuc gagucccgcg aguacaccaa gcaccucauc cgcgucgaga      480

acuggaucuu ccgcaacccc ggcuucgccc ucgccgccgc cgccaucgcc uggcuccucg      540

gcuccuccac cucccagaag gucaucuacc ucgucaugau ccuccucauc gcccccgccu      600

acuccauccg cugcaucggc gucuccaacc gcgacuucgu cgagggcaug uccggcggca      660

ccugggucga cgucguccuc gagcacggcg gcugcgucac cgucauggcc caggacaagc      720

ccaccgucga caucgagcuc gucaccacca ccgucuccaa cauggccgag guccgcuccu      780

acugcuacga ggccuccauc uccgacaugg ccuccgacuc ccgcugcccc acccagggcg      840

aggccuaccu cgacaagcag uccgacaccc aguacgucug caagcgcacc cucgucgacc      900

gcggcugggg caacggcugc ggccucuucg gcaagggcuc ccucgucacc ugcgccaagu      960

ucgccugcuc caagaagaug accggcaagu ccauccagcc cgagaaccuc gaguaccgca     1020

ucaugcucuc cguccacggc ucccagcacu ccggcaugau cgucaacgac accggccacg     1080

agaccgacga gaaccgcgcc aaggucgaga ucacccccaa cuccccccgc gccgaggcca     1140

cccucggcgg cuucggcucc cucggccucg acugcgagcc ccgcaccggc cucgacuucu     1200

ccgaccucua cuaccucacc augaacaaca agcacuggcu cguccacaag gagugguucc     1260

acgacauccc ccuccccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu cgucgaguuc aaggacgccc acgccaagcg ccagaccguc gucguccucg     1380

gcucccagga gggcgccguc cacaccgccc ucgccggcgc ccucgaggcc gagauggacg     1440

gcgccaaggg ccgccucucc uccggccacc ucaagugccg ccucaagaug gacaagcucc     1500

gccucaaggg cgucuccuac ucccucugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu ccacggcacc gucaccgucg agguccagua cgccggcacc gacggccccu     1620

gcaagguccc cgcccagaug gccgucgaca ugcagacccu cacccccguc ggccgccuca     1680

ucaccgccaa ccccgucauc accgagucca ccgagaacuc caagaugaug cucgagcucg     1740

acccccccuu cggcgacucc uacaucguca ucggcgucgg cgagaagaag aucacccacc     1800

acuggcaccg cuccggcucc acccucggca aggccuucuc caccacccuc aagggcgccc     1860

agcgccucgc cgcccucggc gacaccgccu gggacuucgg cuccaucggc ggcgucuuca     1920

acuccaucgg ccgcgccguc caccaggucu ucggcggcgc cuuccgcacc cucuucggcg     1980

gcauguccug gaucacccag ggccucaugg gcgcccuccu ccucuggaug ggcgucaacg     2040

cccgcgaccg cuccaucgcc cucgccuucc ucgccaccgg cggcguccuc gucuuccucg     2100

ccaccaacgu ccacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10421
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10421
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgaca ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac ccucggcaag gccuucucca     1860

ccacccucaa gggcgcccag cgccucgccg cccucggcga caccgccugg gacuucggcu     1920

ccaucggcgg cgucuucaac uccaucggcc gcgccgucca ccaggucuuc ggcggcgccu     1980

uccgcacccu cuucggcggc auguccugga ucacccaggg ccucaugggc gcccuccucc     2040

ucuggauggg cgucaacgcc cgcgaccgcu ccaucgcccu cgccuuccuc gccaccggcg     2100

gcguccucgu cuuccucgcc accaacgucc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10422
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10422
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacac cugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac ccucggcaag gccuucucca     1860

ccacccucaa gggcgcccag cgccucgccg cccucggcga caccgccugg gacuucggcu     1920

ccaucggcgg cgucuucaac uccaucggcc gcgccgucca ccaggucuuc ggcggcgccu     1980

uccgcacccu cuucggcggc auguccugga ucacccaggg ccucaugggc gcccuccucc     2040

ucuggauggg cgucaacgcc cgcgaccgcu ccaucgcccu cgccuuccuc gccaccggcg     2100

gcguccucgu cuuccucgcc accaacgucc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10423
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10423
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccaucac ccgccgcggc uccgccuacu acauguaccu cgaccgcucc gacgccggca      180

aggccaucuc cuucgccacc acccucggcg ucaacaagug ccacguccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucaucaa ggucgagaac uggaucuucc gcaaccccgg cuucgcccuc gucgccgucg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc accugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

gcuacgagac cgacgaggac cgcgccaagg ucgaggucac ccccaacucc ccccgcgccg     1140

aggccacccu cggcggcuuc ggcucccucg gccucgacug cgagccccgc accggccucg     1200

acuucuccga ccucuacuac cucaccauga acaacaagca cuggcucguc cacaaggagu     1260

gguuccacga caucccccuc cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccucguc gaguucaagg acgcccacgc caagcgccag accgucgucg     1380

uccucggcuc ccaggagggc gccguccaca ccgcccucgc cggcgcccuc gaggccgaga     1440

uggacggcgc caagggccgc cucuucuccg gccaccucaa gugccgccuc aagauggaca     1500

agcuccgccu caagggcguc uccuacuccc ucugcaccgc cgccuucacc uucaccaagg     1560

uccccgccga gacccuccac ggcaccguca ccgucgaggu ccaguacgcc ggcaccgacg     1620

gccccugcaa gauccccguc cagauggccg ucgacaugca gacccucacc cccgucggcc     1680

gccucaucac cgccaacccc gucaucaccg aguccaccga gaacuccaag augaugcucg     1740

agcucgaccc ccccuucggc gacuccuaca ucgucaucgg cgucggcgac aagaagauca     1800

cccaccacug gcaccgcucc ggcuccaccc ucggcaaggc cuucuccacc acccucaagg     1860

gcgcccagcg ccucgccgcc cucggcgaca ccgccuggga cuucggcucc aucggcggcg     1920

ucuucaacuc caucggccgc gccguccacc aggucuucgg cggcgccuuc cgcacccucu     1980

ucggcggcau guccuggauc acccagggcc ucaugggcgc ccuccuccuc uggaugggcg     2040

ucaacgcccg cgaccgcucc aucgcccucg ccuuccucgc caccggcggc guccucgucu     2100

uccucgccac caacguccac gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10424
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10424
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgucac ccgccgcggc uccgccuacu acauguaccu cgaccgcaac gacgccggcg      180

aggccaucuc cuuccccacc acccucggca ugaacaagug cuacauccag aucauggacc      240

ucggccacau gugcgacgcc accauguccu acgagugccc caugcucgac gagggcgucg      300

agcccgacga cgucgacugc uggugcaaca ccaccuccac cugggucguc uacggcaccu      360

gccaccacaa gaagggcgag gcccgccgcu cccgccgcgc cgucacccuc cccucccacu      420

ccacccgcaa gcuccagacc cgcucccaga ccuggcucga gucccgcgag uacaccaagc      480

accucauccg cgucgagaac uggaucuucc gcaaccccgg cuucgcccuc gccgccgccg      540

ccaucgccug gcuccucggc uccuccaccu cccagaaggu caucuaccuc gucaugaucc      600

uccucaucgc ccccgccuac uccauccgcu gcaucggcgu cuccaaccgc gacuucgucg      660

agggcauguc cggcggcacc ugggucgacg ucguccucga gcacggcggc ugcgucaccg      720

ucauggccca ggacaagccc accgucgaca ucgagcucgu caccaccacc gucuccaaca      780

uggccgaggu ccgcuccuac ugcuacgagg ccuccaucuc cgacauggcc uccgacuccc      840

gcugccccac ccagggcgag gccuaccucg acaagcaguc cgacacccag uacgucugca      900

agcgcacccu cgucgaccgc ggcuggggca acggcugcgg ccucuucggc aagggcuccc      960

ucgucaccug cgccaaguuc gccugcucca agaagaugac cggcaagucc auccagcccg     1020

agaaccucga guaccgcauc augcucuccg uccacggcuc ccagcacucc ggcaugaucg     1080

ucaacgacac cggccacgag accgacgaga accgcgccaa ggucgagauc acccccaacu     1140

ccccccgcgc cgaggccacc cucggcggcu ucggcucccu cggccucgac ugcgagcccc     1200

gcaccggccu cgacuucucc gaccucuacu accucaccau gaacaacaag cacuggcucg     1260

uccacaagga gugguuccac gacauccccc uccccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccucg ucgaguucaa ggacgcccac gccaagcgcc     1380

agaccgucgu cguccucggc ucccaggagg gcgccgucca caccgcccuc gccggcgccc     1440

ucgaggccga gauggacggc gccaagggcc gccucuccuc cggccaccuc aagugccgcc     1500

ucaagaugga caagcuccgc cucaagggcg ucuccuacuc ccucugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccucc acggcaccgu caccgucgag guccaguacg     1620

ccggcaccga cggccccugc aagguccccg cccagauggc cgucgacaug cagacccuca     1680

cccccgucgg ccgccucauc accgccaacc ccgucaucac cgaguccacc gagaacucca     1740

agaugaugcu cgagcucgac ccccccuucg gcgacuccua caucgucauc ggcgucggcg     1800

agaagaagau cacccaccac uggcaccgcu ccggcuccac ccucggcaag gccuucucca     1860

ccacccucaa gggcgcccag cgccucgccg cccucggcga caccgccugg gacuucggcu     1920

ccaucggcgg cgucuucaac uccaucggcc gcgccgucca ccaggucuuc ggcggcgccu     1980

uccgcacccu cuucggcggc auguccugga ucacccaggg ccucaugggc gcccuccucc     2040

ucuggauggg cgucaacgcc cgcgaccgcu ccaucgcccu cgccuuccuc gccaccggcg     2100

gcguccucgu cuuccucgcc accaacgucc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10425
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10425
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgucacc cuccccuccc      120

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      180

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      240

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      300

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      360

ucgagggcau guccggcggc accugggucg acaucguccu cgagcacggc ggcugcguca      420

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      480

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      540

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      600

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      660

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc      720

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga      780

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca      840

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc      900

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc      960

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1020

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1080

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1140

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1200

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1260

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1320

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1380

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1440

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1500

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1560

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1620

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1680

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     1740

ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc cucgcccucg     1800

gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10426
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10426
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgucacc cuccccuccc      120

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      180

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      240

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      300

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      360

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      420

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      480

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      540

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      600

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      660

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc      720

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga      780

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca      840

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc      900

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc      960

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1020

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1080

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1140

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1200

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1260

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1320

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1380

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1440

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1500

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1560

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1620

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1680

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     1740

ucauguggcu cggccucaac gccaagaacg gcuccaucuc ccucaugugc cucgcccucg     1800

gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10427
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10427
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggcau caucggccuc cuccucacca ccgccauggc cgccgucacc cuccccuccc      120

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      180

agcaccucau caaggucgag aacuggaucu uccgcaaccc cggcuucgcc cucgucgccg      240

ucgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      300

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      360

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      420

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      480

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      540

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      600

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      660

cccucgucac cugcgccaag uucaccugcu ccaagaagau gaccggcaag uccauccagc      720

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga      780

ucggcuacga gaccgacgag gaccgcgcca aggucgaggu cacccccaac uccccccgcg      840

ccgaggccac ccucggcggc uucggcuccc ucggccucga cugcgagccc cgcaccggcc      900

ucgacuucuc cgaccucuac uaccucacca ugaacaacaa gcacuggcuc guccacaagg      960

agugguucca cgacaucccc cuccccuggc acgccggcgc cgacaccggc accccccacu     1020

ggaacaacaa ggaggcccuc gucgaguuca aggacgccca cgccaagcgc cagaccgucg     1080

ucguccucgg cucccaggag ggcgccgucc acaccgcccu cgccggcgcc cucgaggccg     1140

agauggacgg cgccaagggc cgccucuucu ccggccaccu caagugccgc cucaagaugg     1200

acaagcuccg ccucaagggc gucuccuacu cccucugcac cgccgccuuc accuucacca     1260

agguccccgc cgagacccuc cacggcaccg ucaccgucga gguccaguac gccggcaccg     1320

acggccccug caagaucccc guccagaugg ccgucgacau gcagacccuc acccccgucg     1380

gccgccucau caccgccaac cccgucauca ccgaguccac cgagaacucc aagaugaugc     1440

ucgagcucga cccccccuuc ggcgacuccu acaucgucau cggcgucggc gacaagaaga     1500

ucacccacca cuggcaccgc uccggcucca ccaucggcaa ggccuucgag gccaccgucc     1560

gcggcgccaa gcgcauggcc guccucggcg acaccgccug ggacuucggc uccgucggcg     1620

gcgucuucaa cucccucggc aagggcaucc accagaucuu cggcgccgcc uucaaguccc     1680

ucuucggcgg cauguccugg uucucccaga uccucaucgg cacccuccuc gucuggcucg     1740

gccucaacac caagaacggc uccaucuccc ucaccugccu cgcccucggc ggcgucauga     1800

ucuuccucuc caccgccguc uccgccugag gacuagugca ucacauuuaa aagcaucuca     1860

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     1920

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     1980

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2100

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2158


<210>  10428
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10428
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccgucggcau cgucggccuc cuccucacca ccgccauggc cgccgucacc cuccccuccc      120

acuccacccg caagcuccag acccgcuccc agaccuggcu cgagucccgc gaguacacca      180

agcaccucau ccgcgucgag aacuggaucu uccgcaaccc cggcuucgcc cucgccgccg      240

ccgccaucgc cuggcuccuc ggcuccucca ccucccagaa ggucaucuac cucgucauga      300

uccuccucau cgcccccgcc uacuccaucc gcugcaucgg cgucuccaac cgcgacuucg      360

ucgagggcau guccggcggc accugggucg acgucguccu cgagcacggc ggcugcguca      420

ccgucauggc ccaggacaag cccaccgucg acaucgagcu cgucaccacc accgucucca      480

acauggccga gguccgcucc uacugcuacg aggccuccau cuccgacaug gccuccgacu      540

cccgcugccc cacccagggc gaggccuacc ucgacaagca guccgacacc caguacgucu      600

gcaagcgcac ccucgucgac cgcggcuggg gcaacggcug cggccucuuc ggcaagggcu      660

cccucgucac cugcgccaag uucgccugcu ccaagaagau gaccggcaag uccauccagc      720

ccgagaaccu cgaguaccgc aucaugcucu ccguccacgg cucccagcac uccggcauga      780

ucgucaacga caccggccac gagaccgacg agaaccgcgc caaggucgag aucaccccca      840

acuccccccg cgccgaggcc acccucggcg gcuucggcuc ccucggccuc gacugcgagc      900

cccgcaccgg ccucgacuuc uccgaccucu acuaccucac caugaacaac aagcacuggc      960

ucguccacaa ggagugguuc cacgacaucc cccuccccug gcacgccggc gccgacaccg     1020

gcacccccca cuggaacaac aaggaggccc ucgucgaguu caaggacgcc cacgccaagc     1080

gccagaccgu cgucguccuc ggcucccagg agggcgccgu ccacaccgcc cucgccggcg     1140

cccucgaggc cgagauggac ggcgccaagg gccgccucuc cuccggccac cucaagugcc     1200

gccucaagau ggacaagcuc cgccucaagg gcgucuccua cucccucugc accgccgccu     1260

ucaccuucac caagaucccc gccgagaccc uccacggcac cgucaccguc gagguccagu     1320

acgccggcac cgacggcccc ugcaaggucc ccgcccagau ggccgucgac augcagaccc     1380

ucacccccgu cggccgccuc aucaccgcca accccgucau caccgagucc accgagaacu     1440

ccaagaugau gcucgagcuc gacccccccu ucggcgacuc cuacaucguc aucggcgucg     1500

gcgagaagaa gaucacccac cacuggcacc gcuccggcuc caccaucggc aaggccuucg     1560

aggccaccgu ccgcggcgcc aagcgcaugg ccguccucgg cgacaccgcc ugggacuucg     1620

gcuccgucgg cggcgcccuc aacucccucg gcaagggcau ccaccagauc uucggcgccg     1680

ccuucaaguc ccucuucggc ggcauguccu gguucuccca gauccucauc ggcacccucc     1740

ucauguggcu cggccucaac accaagaacg gcuccaucuc ccucaugugc cucgcccucg     1800

gcggcguccu caucuuccuc uccaccgccg ucuccgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10429
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10429
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc cgcucccaga      180

ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac uggaucuucc      240

gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc uccuccaccu      300

cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac uccauccgcu      360

gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc ugggucgaca      420

ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc accgucgaca      480

ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg      540

ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag gccuaccucg      600

acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc ggcuggggca      660

acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc gccugcucca      720

agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc augcucuccg      780

uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag accgacgaga      840

accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc cucggcggcu      900

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu      960

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1020

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1080

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1140

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1200

gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1260

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc gagacccucc     1320

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagguccccg     1380

cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1440

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1500

gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac uggcaccgcu     1560

ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag cgcauggccg     1620

uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac ucccucggca     1680

agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc auguccuggu     1740

ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc aagaacggcu     1800

ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc accgccgucu     1860

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10430
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10430
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc cgcucccaga      180

ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac uggaucuucc      240

gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc uccuccaccu      300

cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac uccauccgcu      360

gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc ugggucgacg      420

ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc accgucgaca      480

ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg      540

ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag gccuaccucg      600

acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc ggcuggggca      660

acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc gccugcucca      720

agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc augcucuccg      780

uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag accgacgaga      840

accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc cucggcggcu      900

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu      960

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1020

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1080

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1140

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1200

gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1260

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc gagacccucc     1320

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagguccccg     1380

cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1440

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1500

gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac uggcaccgcu     1560

ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag cgcauggccg     1620

uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac ucccucggca     1680

agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc auguccuggu     1740

ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacgcc aagaacggcu     1800

ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc accgccgucu     1860

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10431
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10431
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc cgcucccaga      180

ccuggcucga gucccgcgag uacaccaagc accucaucaa ggucgagaac uggaucuucc      240

gcaaccccgg cuucgcccuc gucgccgucg ccaucgccug gcuccucggc uccuccaccu      300

cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac uccauccgcu      360

gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc ugggucgacg      420

ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc accgucgaca      480

ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg      540

ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag gccuaccucg      600

acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc ggcuggggca      660

acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc accugcucca      720

agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc augcucuccg      780

uccacggcuc ccagcacucc ggcaugaucg gcuacgagac cgacgaggac cgcgccaagg      840

ucgaggucac ccccaacucc ccccgcgccg aggccacccu cggcggcuuc ggcucccucg      900

gccucgacug cgagccccgc accggccucg acuucuccga ccucuacuac cucaccauga      960

acaacaagca cuggcucguc cacaaggagu gguuccacga caucccccuc cccuggcacg     1020

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccucguc gaguucaagg     1080

acgcccacgc caagcgccag accgucgucg uccucggcuc ccaggagggc gccguccaca     1140

ccgcccucgc cggcgcccuc gaggccgaga uggacggcgc caagggccgc cucuucuccg     1200

gccaccucaa gugccgccuc aagauggaca agcuccgccu caagggcguc uccuacuccc     1260

ucugcaccgc cgccuucacc uucaccaagg uccccgccga gacccuccac ggcaccguca     1320

ccgucgaggu ccaguacgcc ggcaccgacg gccccugcaa gauccccguc cagauggccg     1380

ucgacaugca gacccucacc cccgucggcc gccucaucac cgccaacccc gucaucaccg     1440

aguccaccga gaacuccaag augaugcucg agcucgaccc ccccuucggc gacuccuaca     1500

ucgucaucgg cgucggcgac aagaagauca cccaccacug gcaccgcucc ggcuccacca     1560

ucggcaaggc cuucgaggcc accguccgcg gcgccaagcg cauggccguc cucggcgaca     1620

ccgccuggga cuucggcucc gucggcggcg ucuucaacuc ccucggcaag ggcauccacc     1680

agaucuucgg cgccgccuuc aagucccucu ucggcggcau guccugguuc ucccagaucc     1740

ucaucggcac ccuccucguc uggcucggcc ucaacaccaa gaacggcucc aucucccuca     1800

ccugccucgc ccucggcggc gucaugaucu uccucuccac cgccgucucc gccugaggac     1860

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     1920

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     1980

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2040

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2160

ggcucuuuuc agagccacca gaauu                                           2185


<210>  10432
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10432
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucuccggcgu ccccguccuc ggcuucuuca ucaucgccgu ccucaugucc gcccaggagu      120

ccugggccgc cgucacccuc cccucccacu ccacccgcaa gcuccagacc cgcucccaga      180

ccuggcucga gucccgcgag uacaccaagc accucauccg cgucgagaac uggaucuucc      240

gcaaccccgg cuucgcccuc gccgccgccg ccaucgccug gcuccucggc uccuccaccu      300

cccagaaggu caucuaccuc gucaugaucc uccucaucgc ccccgccuac uccauccgcu      360

gcaucggcgu cuccaaccgc gacuucgucg agggcauguc cggcggcacc ugggucgacg      420

ucguccucga gcacggcggc ugcgucaccg ucauggccca ggacaagccc accgucgaca      480

ucgagcucgu caccaccacc gucuccaaca uggccgaggu ccgcuccuac ugcuacgagg      540

ccuccaucuc cgacauggcc uccgacuccc gcugccccac ccagggcgag gccuaccucg      600

acaagcaguc cgacacccag uacgucugca agcgcacccu cgucgaccgc ggcuggggca      660

acggcugcgg ccucuucggc aagggcuccc ucgucaccug cgccaaguuc gccugcucca      720

agaagaugac cggcaagucc auccagcccg agaaccucga guaccgcauc augcucuccg      780

uccacggcuc ccagcacucc ggcaugaucg ucaacgacac cggccacgag accgacgaga      840

accgcgccaa ggucgagauc acccccaacu ccccccgcgc cgaggccacc cucggcggcu      900

ucggcucccu cggccucgac ugcgagcccc gcaccggccu cgacuucucc gaccucuacu      960

accucaccau gaacaacaag cacuggcucg uccacaagga gugguuccac gacauccccc     1020

uccccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccucg     1080

ucgaguucaa ggacgcccac gccaagcgcc agaccgucgu cguccucggc ucccaggagg     1140

gcgccgucca caccgcccuc gccggcgccc ucgaggccga gauggacggc gccaagggcc     1200

gccucuccuc cggccaccuc aagugccgcc ucaagaugga caagcuccgc cucaagggcg     1260

ucuccuacuc ccucugcacc gccgccuuca ccuucaccaa gauccccgcc gagacccucc     1320

acggcaccgu caccgucgag guccaguacg ccggcaccga cggccccugc aagguccccg     1380

cccagauggc cgucgacaug cagacccuca cccccgucgg ccgccucauc accgccaacc     1440

ccgucaucac cgaguccacc gagaacucca agaugaugcu cgagcucgac ccccccuucg     1500

gcgacuccua caucgucauc ggcgucggcg agaagaagau cacccaccac uggcaccgcu     1560

ccggcuccac caucggcaag gccuucgagg ccaccguccg cggcgccaag cgcauggccg     1620

uccucggcga caccgccugg gacuucggcu ccgucggcgg cgcccucaac ucccucggca     1680

agggcaucca ccagaucuuc ggcgccgccu ucaagucccu cuucggcggc auguccuggu     1740

ucucccagau ccucaucggc acccuccuca uguggcucgg ccucaacacc aagaacggcu     1800

ccaucucccu caugugccuc gcccucggcg gcguccucau cuuccucucc accgccgucu     1860

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10433
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10433
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccgcc gucacccucc      120

ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag ucccgcgagu      180

acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc uucgcccucg      240

ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc aucuaccucg      300

ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc uccaaccgcg      360

acuucgucga gggcaugucc ggcggcaccu gggucgacau cguccucgag cacggcggcu      420

gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc accaccaccg      480

ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc gacauggccu      540

ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc gacacccagu      600

acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc cucuucggca      660

agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc ggcaagucca      720

uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc cagcacuccg      780

gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag gucgagauca      840

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu      900

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc      960

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1020

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1080

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1140

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca     1200

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1260

ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc accgucgagg     1320

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1380

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1440

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1500

gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1560

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1620

acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac cagaucuucg     1680

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     1740

cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc augugccucg     1800

cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10434
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10434
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccgcc gucacccucc      120

ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag ucccgcgagu      180

acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc uucgcccucg      240

ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc aucuaccucg      300

ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc uccaaccgcg      360

acuucgucga gggcaugucc ggcggcaccu gggucgacgu cguccucgag cacggcggcu      420

gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc accaccaccg      480

ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc gacauggccu      540

ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc gacacccagu      600

acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc cucuucggca      660

agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc ggcaagucca      720

uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc cagcacuccg      780

gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag gucgagauca      840

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu      900

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc      960

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1020

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1080

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1140

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca     1200

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1260

ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc accgucgagg     1320

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1380

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1440

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1500

gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1560

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1620

acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac cagaucuucg     1680

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     1740

cccuccucau guggcucggc cucaacgcca agaacggcuc caucucccuc augugccucg     1800

cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10435
<211>  2164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10435
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccgcc gucacccucc      120

ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag ucccgcgagu      180

acaccaagca ccucaucaag gucgagaacu ggaucuuccg caaccccggc uucgcccucg      240

ucgccgucgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc aucuaccucg      300

ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc uccaaccgcg      360

acuucgucga gggcaugucc ggcggcaccu gggucgacgu cguccucgag cacggcggcu      420

gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc accaccaccg      480

ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc gacauggccu      540

ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc gacacccagu      600

acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc cucuucggca      660

agggcucccu cgucaccugc gccaaguuca ccugcuccaa gaagaugacc ggcaagucca      720

uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc cagcacuccg      780

gcaugaucgg cuacgagacc gacgaggacc gcgccaaggu cgaggucacc cccaacuccc      840

cccgcgccga ggccacccuc ggcggcuucg gcucccucgg ccucgacugc gagccccgca      900

ccggccucga cuucuccgac cucuacuacc ucaccaugaa caacaagcac uggcucgucc      960

acaaggagug guuccacgac aucccccucc ccuggcacgc cggcgccgac accggcaccc     1020

cccacuggaa caacaaggag gcccucgucg aguucaagga cgcccacgcc aagcgccaga     1080

ccgucgucgu ccucggcucc caggagggcg ccguccacac cgcccucgcc ggcgcccucg     1140

aggccgagau ggacggcgcc aagggccgcc ucuucuccgg ccaccucaag ugccgccuca     1200

agauggacaa gcuccgccuc aagggcgucu ccuacucccu cugcaccgcc gccuucaccu     1260

ucaccaaggu ccccgccgag acccuccacg gcaccgucac cgucgagguc caguacgccg     1320

gcaccgacgg ccccugcaag auccccgucc agauggccgu cgacaugcag acccucaccc     1380

ccgucggccg ccucaucacc gccaaccccg ucaucaccga guccaccgag aacuccaaga     1440

ugaugcucga gcucgacccc cccuucggcg acuccuacau cgucaucggc gucggcgaca     1500

agaagaucac ccaccacugg caccgcuccg gcuccaccau cggcaaggcc uucgaggcca     1560

ccguccgcgg cgccaagcgc auggccgucc ucggcgacac cgccugggac uucggcuccg     1620

ucggcggcgu cuucaacucc cucggcaagg gcauccacca gaucuucggc gccgccuuca     1680

agucccucuu cggcggcaug uccugguucu cccagauccu caucggcacc cuccucgucu     1740

ggcucggccu caacaccaag aacggcucca ucucccucac cugccucgcc cucggcggcg     1800

ucaugaucuu ccucuccacc gccgucuccg ccugaggacu agugcaucac auuuaaaagc     1860

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     1920

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     1980

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2160

aauu                                                                  2164


<210>  10436
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10436
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc gucgccgccg ccacccgcgu ccacuccgcc gucacccucc      120

ccucccacuc cacccgcaag cuccagaccc gcucccagac cuggcucgag ucccgcgagu      180

acaccaagca ccucauccgc gucgagaacu ggaucuuccg caaccccggc uucgcccucg      240

ccgccgccgc caucgccugg cuccucggcu ccuccaccuc ccagaagguc aucuaccucg      300

ucaugauccu ccucaucgcc cccgccuacu ccauccgcug caucggcguc uccaaccgcg      360

acuucgucga gggcaugucc ggcggcaccu gggucgacgu cguccucgag cacggcggcu      420

gcgucaccgu cauggcccag gacaagccca ccgucgacau cgagcucguc accaccaccg      480

ucuccaacau ggccgagguc cgcuccuacu gcuacgaggc cuccaucucc gacauggccu      540

ccgacucccg cugccccacc cagggcgagg ccuaccucga caagcagucc gacacccagu      600

acgucugcaa gcgcacccuc gucgaccgcg gcuggggcaa cggcugcggc cucuucggca      660

agggcucccu cgucaccugc gccaaguucg ccugcuccaa gaagaugacc ggcaagucca      720

uccagcccga gaaccucgag uaccgcauca ugcucuccgu ccacggcucc cagcacuccg      780

gcaugaucgu caacgacacc ggccacgaga ccgacgagaa ccgcgccaag gucgagauca      840

cccccaacuc cccccgcgcc gaggccaccc ucggcggcuu cggcucccuc ggccucgacu      900

gcgagccccg caccggccuc gacuucuccg accucuacua ccucaccaug aacaacaagc      960

acuggcucgu ccacaaggag ugguuccacg acaucccccu ccccuggcac gccggcgccg     1020

acaccggcac cccccacugg aacaacaagg aggcccucgu cgaguucaag gacgcccacg     1080

ccaagcgcca gaccgucguc guccucggcu cccaggaggg cgccguccac accgcccucg     1140

ccggcgcccu cgaggccgag auggacggcg ccaagggccg ccucuccucc ggccaccuca     1200

agugccgccu caagauggac aagcuccgcc ucaagggcgu cuccuacucc cucugcaccg     1260

ccgccuucac cuucaccaag auccccgccg agacccucca cggcaccguc accgucgagg     1320

uccaguacgc cggcaccgac ggccccugca agguccccgc ccagauggcc gucgacaugc     1380

agacccucac ccccgucggc cgccucauca ccgccaaccc cgucaucacc gaguccaccg     1440

agaacuccaa gaugaugcuc gagcucgacc cccccuucgg cgacuccuac aucgucaucg     1500

gcgucggcga gaagaagauc acccaccacu ggcaccgcuc cggcuccacc aucggcaagg     1560

ccuucgaggc caccguccgc ggcgccaagc gcauggccgu ccucggcgac accgccuggg     1620

acuucggcuc cgucggcggc gcccucaacu cccucggcaa gggcauccac cagaucuucg     1680

gcgccgccuu caagucccuc uucggcggca uguccugguu cucccagauc cucaucggca     1740

cccuccucau guggcucggc cucaacacca agaacggcuc caucucccuc augugccucg     1800

cccucggcgg cguccucauc uuccucucca ccgccgucuc cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10437
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10437
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      180

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      240

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      300

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      360

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacaucg      420

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      480

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      540

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      600

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      660

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      720

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      780

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      840

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      900

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      960

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1020

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1080

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1140

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1200

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1260

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1320

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1380

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1440

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1500

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1560

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1620

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1680

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     1740

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     1800

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10438
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10438
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      180

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      240

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      300

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      360

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      420

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      480

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      540

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      600

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      660

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      720

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      780

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      840

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      900

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      960

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1020

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1080

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1140

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1200

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1260

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1320

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1380

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1440

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1500

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1560

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1620

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1680

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     1740

cccagauccu caucggcacc cuccucaugu ggcucggccu caacgccaag aacggcucca     1800

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10439
<211>  2182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10439
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      180

ggcucgaguc ccgcgaguac accaagcacc ucaucaaggu cgagaacugg aucuuccgca      240

accccggcuu cgcccucguc gccgucgcca ucgccuggcu ccucggcucc uccaccuccc      300

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      360

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      420

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      480

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      540

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      600

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      660

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucacc ugcuccaaga      720

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      780

acggcuccca gcacuccggc augaucggcu acgagaccga cgaggaccgc gccaaggucg      840

aggucacccc caacuccccc cgcgccgagg ccacccucgg cggcuucggc ucccucggcc      900

ucgacugcga gccccgcacc ggccucgacu ucuccgaccu cuacuaccuc accaugaaca      960

acaagcacug gcucguccac aaggaguggu uccacgacau cccccucccc uggcacgccg     1020

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccucgucgag uucaaggacg     1080

cccacgccaa gcgccagacc gucgucgucc ucggcuccca ggagggcgcc guccacaccg     1140

cccucgccgg cgcccucgag gccgagaugg acggcgccaa gggccgccuc uucuccggcc     1200

accucaagug ccgccucaag auggacaagc uccgccucaa gggcgucucc uacucccucu     1260

gcaccgccgc cuucaccuuc accaaggucc ccgccgagac ccuccacggc accgucaccg     1320

ucgaggucca guacgccggc accgacggcc ccugcaagau ccccguccag auggccgucg     1380

acaugcagac ccucaccccc gucggccgcc ucaucaccgc caaccccguc aucaccgagu     1440

ccaccgagaa cuccaagaug augcucgagc ucgacccccc cuucggcgac uccuacaucg     1500

ucaucggcgu cggcgacaag aagaucaccc accacuggca ccgcuccggc uccaccaucg     1560

gcaaggccuu cgaggccacc guccgcggcg ccaagcgcau ggccguccuc ggcgacaccg     1620

ccugggacuu cggcuccguc ggcggcgucu ucaacucccu cggcaagggc auccaccaga     1680

ucuucggcgc cgccuucaag ucccucuucg gcggcauguc cugguucucc cagauccuca     1740

ucggcacccu ccucgucugg cucggccuca acaccaagaa cggcuccauc ucccucaccu     1800

gccucgcccu cggcggcguc augaucuucc ucuccaccgc cgucuccgcc ugaggacuag     1860

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     1920

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     1980

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2040

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2160

ucuuuucaga gccaccagaa uu                                              2182


<210>  10440
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10440
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgcuccgc cggcuccauc auguggcucg ccucccucgc cgucgucauc gccugcgccg      120

gcgccgccgu cacccucccc ucccacucca cccgcaagcu ccagacccgc ucccagaccu      180

ggcucgaguc ccgcgaguac accaagcacc ucauccgcgu cgagaacugg aucuuccgca      240

accccggcuu cgcccucgcc gccgccgcca ucgccuggcu ccucggcucc uccaccuccc      300

agaaggucau cuaccucguc augauccucc ucaucgcccc cgccuacucc auccgcugca      360

ucggcgucuc caaccgcgac uucgucgagg gcauguccgg cggcaccugg gucgacgucg      420

uccucgagca cggcggcugc gucaccguca uggcccagga caagcccacc gucgacaucg      480

agcucgucac caccaccguc uccaacaugg ccgagguccg cuccuacugc uacgaggccu      540

ccaucuccga cauggccucc gacucccgcu gccccaccca gggcgaggcc uaccucgaca      600

agcaguccga cacccaguac gucugcaagc gcacccucgu cgaccgcggc uggggcaacg      660

gcugcggccu cuucggcaag ggcucccucg ucaccugcgc caaguucgcc ugcuccaaga      720

agaugaccgg caaguccauc cagcccgaga accucgagua ccgcaucaug cucuccgucc      780

acggcuccca gcacuccggc augaucguca acgacaccgg ccacgagacc gacgagaacc      840

gcgccaaggu cgagaucacc cccaacuccc cccgcgccga ggccacccuc ggcggcuucg      900

gcucccucgg ccucgacugc gagccccgca ccggccucga cuucuccgac cucuacuacc      960

ucaccaugaa caacaagcac uggcucgucc acaaggagug guuccacgac aucccccucc     1020

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccucgucg     1080

aguucaagga cgcccacgcc aagcgccaga ccgucgucgu ccucggcucc caggagggcg     1140

ccguccacac cgcccucgcc ggcgcccucg aggccgagau ggacggcgcc aagggccgcc     1200

ucuccuccgg ccaccucaag ugccgccuca agauggacaa gcuccgccuc aagggcgucu     1260

ccuacucccu cugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccuccacg     1320

gcaccgucac cgucgagguc caguacgccg gcaccgacgg ccccugcaag guccccgccc     1380

agauggccgu cgacaugcag acccucaccc ccgucggccg ccucaucacc gccaaccccg     1440

ucaucaccga guccaccgag aacuccaaga ugaugcucga gcucgacccc cccuucggcg     1500

acuccuacau cgucaucggc gucggcgaga agaagaucac ccaccacugg caccgcuccg     1560

gcuccaccau cggcaaggcc uucgaggcca ccguccgcgg cgccaagcgc auggccgucc     1620

ucggcgacac cgccugggac uucggcuccg ucggcggcgc ccucaacucc cucggcaagg     1680

gcauccacca gaucuucggc gccgccuuca agucccucuu cggcggcaug uccugguucu     1740

cccagauccu caucggcacc cuccucaugu ggcucggccu caacaccaag aacggcucca     1800

ucucccucau gugccucgcc cucggcggcg uccucaucuu ccucuccacc gccgucuccg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10441
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10441
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgu gaccagaagg ggaucagcgu acuauaugua ccuugaccgg aacgaugcug      180

gugaagccau caguuucccu acaacucugg gcaugaauaa gugcuauauu caaaucaugg      240

acuuggggca caugugugau gcaacgauga gcuacgagug cccaauguua gacgaaggag      300

uagagccgga ugacguggau uguuggugca acaccacauc cacuuggguc guguauggca      360

ccugucauca caaaaagggu gaagcccgcc gaucucguag agcuguuaca cugcccucgc      420

auucaaccag gaaacuacag acucggagcc agacauggcu cgagagucgc gaguacacca      480

agcaccugau uagaguggaa aauuggaucu uuaggaaccc uggguucgca cuugccgcug      540

ccgcgauagc auggcuguug ggauccucua cgagccagaa agucaucuau cucgugauga      600

uucugcugau cgccccagcu uacuccauuc ggugcaucgg cguaucaaau cgcgacuuug      660

uugagggcau gaguggggga acuugggugg auauaguccu cgaacauggu ggcuguguga      720

ccgugauggc ccaagacaag cccacagucg auauugaguu aguuacuacc acagugagca      780

acauggcaga gguacgaucu uacugcuaug aggcuuccau cucagacaug gccagcgaua      840

guagaugucc uacccagggg gaagcguacc uggacaagca gucugacacg caguaugugu      900

gcaaaaggac ucuugucgau cggggauggg gcaaugguug uggguuguuc ggaaagggcu      960

cccuggugac augcgccaaa uuugcaugca gcaagaaaau gaccggcaag ucgauucaac     1020

cagaaaaccu agaguaccgu aucaugcucu cuguucacgg gucccagcac ucaggaauga     1080

ucgugaauga cacuggucau gaaacagaug agaaccgcgc uaaggucgag auuaccccca     1140

auaguccuag agccgaagcu acgcugggcg gguucggaag ccuuggccug gacugugagc     1200

caaggaccgg uuuggauuuu uccgaccucu auuaccugac aaugaacaau aaacacuggu     1260

uagugcauaa ggaaugguuc cacgauauac cgcugcccug gcacgccggg gcagacacug     1320

gcaccccuca uuggaacaau aaagaggccc ucguagaauu uaaggaugcu cacgcgaaac     1380

ggcagacagu ugugguccuu ggaucucagg agggcgcagu gcauacugcc cuggcugggg     1440

ccuuggaggc agaaauggac ggagccaagg gucgacuaag cucaggccac cugaaaugcc     1500

gccucaagau ggauaagcug agacuuaaag gggucaguua uucccugugu accgcugccu     1560

ucacauuuac caagauccca gcagagacuu ugcauggaac ggugacaguu gaagugcaau     1620

acgcgggcac cgacgguccc ugcaaaguac cugcucagau ggccgucgac augcagacuc     1680

ucaccccagu ggggaggcug auuacagcaa accccguuau caccgagagc acugaaaacu     1740

cuaagaugau guuagagcug gauccgccuu ucggcgacuc auacauagug auuggagucg     1800

gcgagaaaaa gaucacacac cacuggcauc ggagugggag cacgaucgga aaggccuucg     1860

aagcuaccgu gcgcggugcc aaaagaaugg caguacuagg cgauacugcu ugggacuuug     1920

gguccguggg aggcgcccuc aauucucugg guaaggggau ucaccaaauc uucggcgcgg     1980

ccuuuaaauc gcuuuucgga ggcaugagcu gguuuuccca gauuuugaua gggacacugc     2040

ucauguggcu gggacuuaac accaagaaug gcucaaucag ucugaugugu uuggcucucg     2100

gugggguucu gauuuucuua ucuacagcag ucagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10442
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10442
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgu gaccagaagg ggaucagcgu acuauaugua ccuugaccgg aacgaugcug      180

gugaagccau caguuucccu acaacucugg gcaugaauaa gugcuauauu caaaucaugg      240

acuuggggca cacgugugau gcaaccauga gcuacgagug cccaauguua gacgaaggag      300

uagagccgga ugacguggau uguuggugca acacaacuuc caccuggguc guguauggca      360

caugucauca caaaaagggu gaagcccgcc gaucucguag agcuguuacc cugcccucgc      420

auucaacuag gaaacuacag acacggagcc agaccuggcu cgagagucgc gaguacacga      480

agcaccugau uagaguggaa aauuggaucu uuaggaaccc uggguucgca cuugccgcug      540

ccgcgauagc auggcuguug ggauccucua cuagccagaa agucaucuau cucgugauga      600

uucugcugau cgccccagcu uacuccauuc ggugcaucgg cguaucaaau cgcgacuuug      660

uugagggcau gaguggggga accugggugg augucgugcu cgaacauggu ggcuguguga      720

cagucauggc ccaagacaag cccacuguug auauagaguu agugaccaca accguaagca      780

acauggcaga ggugcgaucu uacugcuaug aggcuuccau uucagacaug gccagcgaua      840

guagaugucc uacgcagggg gaagcguacc uggacaagca gucugacacu caguaugucu      900

gcaaaaggac acuuguggau cggggauggg gcaaugguug uggguuguuc ggaaagggcu      960

cccugguuac cugcgccaaa uuugcaugca gcaagaaaau gacuggcaag ucgauccaac     1020

cagaaaaccu agaguaccgu auuaugcucu cugugcacgg gucccagcac ucaggaauga     1080

ucgucaauga cacaggucau gaaaccgaug agaaccgcgc uaagguggag aucacgccca     1140

auaguccuag agccgaagcu acccugggcg gguucggaag ccuuggccug gacugugagc     1200

caaggacagg uuuggauuuu uccgaccucu auuaccugac uaugaacaau aaacacuggu     1260

uaguacauaa ggaaugguuc cacgauauuc cgcugcccug gcacgccggg gcagacaccg     1320

gcacaccuca uuggaacaau aaagaggccc ucguugaauu uaaggaugcu cacgcgaaac     1380

ggcagacugu ggucgugcuu ggaucucagg agggcgcagu ccauaccgcc cuggcugggg     1440

ccuuggaggc agaaauggac ggagccaagg gucgacuaag cucaggccac cugaaaugcc     1500

gccucaagau ggauaagcug agacuuaaag gggugaguua uucccugugu acagcugccu     1560

ucaccuuuac uaagauacca gcagagacgu ugcauggaac aguuaccgug gaaguacaau     1620

acgcgggcac ugacgguccc ugcaaagucc cugcucagau ggccguggac augcagaccc     1680

ucacaccagu ugggaggcug aucaccgcaa accccgugau uacugagagc acagaaaacu     1740

cuaagaugau guuagagcug gauccgccuu ucggcgacuc auacaucguc auaggagugg     1800

gcgagaaaaa gauuacgcac cacuggcauc ggagugggag caccaucgga aaggccuucg     1860

aagcuacugu acgcggugcc aaaagaaugg cagugcuagg cgauacagcu ugggacuuug     1920

gguccguugg aggcgcccuc aauucucugg guaaggggau ccaccaaauu uucggcgcgg     1980

ccuuuaaauc gcuuuucgga ggcaugagcu gguuuuccca gaucuugauu gggacccugc     2040

ucauguggcu gggacuuaac gcuaagaaug gcucaauaag ucugaugugu uuggcacucg     2100

gugggguccu gaucuucuua ucuacagccg ugagcgcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10443
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10443
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccau caccagaagg ggaucagcgu acuauaugua ccuugaccgg agugaugcug      180

guaaggccau uagcuucgca acaacucugg gcgugaacaa augccacgua caaaucaugg      240

acuuggggca uaugugugau gccacgaugu ccuaugaaug cccuauguua gacgagggag      300

uggaaccaga ugacgucgau uguuggugca auaccacauc uacuugggug guuuacggca      360

ccugucacca uaagaaaggu gaggcucgcc gaucgcguag agcagugaca cugccgucac      420

acagcaccag gaagcuacag acucggaguc agacauggcu cgaaucccgc gaguauacca      480

aacaucugau uaaggucgag aacuggaucu uuagaaaucc cggguucgcc cuuguggcug      540

uagccauagc guggcuguug ggaucuagca cgucccagaa gguuaucuac cucgugauga      600

uucugcugau cgcacccgcc uauucaauua ggugcaucgg cgucaguaac cgggacuuug      660

uggaaggcau gagcggggga acuugggugg augucguucu cgagcacggu ggcuguguga      720

ccguaauggc ucaagacaaa ccaacagugg auauagaauu agucacuacc acagugucua      780

auauggccga gguucgcucc uacugcuacg aggcaucaau uagcgacaug gcuagugauu      840

cucgaugucc cacccagggg gaagccuauc uggacaagca guccgacacg caguacgugu      900

gcaaaagaac ucuugucgau aggggauggg gcaacgguug uggguuguuc ggaaagggca      960

gccuggugac augcgcgaaa uuuaccugcu cgaagaagau gacuggcaaa ucuauccaac     1020

cugagaaucu agaauaucgg auuaugcucu ccguacacgg gucacagcau aguggaauga     1080

ucgguuacga gacagacgaa gaucgugcca agguugaggu gaccccaaac agcccccgcg     1140

cagaggcuac gcugggcggg uucggauccc uuggccugga cugugaaccu agaaccgguu     1200

uggauuuuuc ugaccucuau uaccugacaa ugaauaacaa acacugguua guccauaagg     1260

agugguucca cgauauccca cugccguggc acgccggggc ugacacuggc accccucauu     1320

ggaauaacaa agaagcccuc guggaguuua aggaugcaca cgccaaaagg cagacagucg     1380

ugguucuugg aagccaggaa ggcgcugugc auacugcgcu ggcaggggcc uuggaggcug     1440

agauggacgg agccaagggu cggcuauucu caggccaccu gaagugccga cucaaaaugg     1500

auaagcugcg ccuuaaaggg guaaguuauu cccuguguac cgcagccuuu acauucacca     1560

aggucccugc ugaaacuuug cauggaacgg ugacaguuga ggugcaauac gccggcaccg     1620

acgguccaug caaaauuccc guccagaugg caguggacau gcagacucuc accccuguag     1680

ggagacugau aacagcgaau ccagugauca ccgaaagcac ugagaacucu aagaugaugu     1740

uagaacugga ucccccguuc ggcgacucau acauuguuau cggagucggc gauaagaaaa     1800

uaacacacca cuggcauagg agugggagca cgauuggaaa ggcuuuugag gccaccgugc     1860

ggggugcaaa acgcauggcc gugcuaggcg acacugcuug ggauuucggg uccgucggag     1920

gcguuuuuaa uucucucggu aaggggaucc accaaaucuu cggcgccgca uuuaaaucgc     1980

uguucggagg caugagcugg uuuucccaga uucuuaucgg gacauugcug guguggcucg     2040

gacugaacac caagaacggc ucaauuaguc uuacaugucu ggcuuugggu ggggugauga     2100

uauuccucuc uacugccgua agcgcgugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10444
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10444
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgu gaccagaagg ggaucagcgu acuauaugua ccuugaccgg aacgaugcug      180

gugaagccau caguuucccu acaacucugg gcaugaauaa gugcuauauu caaaucaugg      240

acuuggggca caugugugau gcaacgauga gcuacgagug cccaauguua gacgaaggag      300

uagagccgga ugacguggau uguuggugca acaccacauc cacuuggguc guguauggca      360

ccugucauca caaaaagggu gaagcccgcc gaucucguag agcuguuaca cugcccucgc      420

auucaaccag gaaacuacag acucggagcc agacauggcu cgagagucgc gaguacacca      480

agcaccugau uagaguggaa aauuggaucu uuaggaaccc uggguucgca cuugccgcug      540

ccgcgauagc auggcuguug ggauccucua cgagccagaa agucaucuau cucgugauga      600

uucugcugau cgccccagcu uacuccauuc ggugcaucgg cguaucaaau cgcgacuuug      660

uugagggcau gaguggggga acuugggugg augucgugcu cgaacauggu ggcuguguga      720

ccgucauggc ccaagacaag cccacaguug auauagaguu agugacuacc acaguaagca      780

acauggcaga ggugcgaucu uacugcuaug aggcuuccau uucagacaug gccagcgaua      840

guagaugucc uacccagggg gaagcguacc uggacaagca gucugacacg caguaugucu      900

gcaaaaggac ucuuguggau cggggauggg gcaaugguug uggguuguuc ggaaagggcu      960

cccugguuac augcgccaaa uuugcaugca gcaagaaaau gaccggcaag ucgauccaac     1020

cagaaaaccu agaguaccgu auuaugcucu cugugcacgg gucccagcac ucaggaauga     1080

ucgucaauga cacuggucau gaaacagaug agaaccgcgc uaagguggag aucaccccca     1140

auaguccuag agccgaagcu acgcugggcg gguucggaag ccuuggccug gacugugagc     1200

caaggaccgg uuuggauuuu uccgaccucu auuaccugac aaugaacaau aaacacuggu     1260

uaguacauaa ggaaugguuc cacgauauuc cgcugcccug gcacgccggg gcagacacug     1320

gcaccccuca uuggaacaau aaagaggccc ucguugaauu uaaggaugcu cacgcgaaac     1380

ggcagacagu ggucgugcuu ggaucucagg agggcgcagu ccauacugcc cuggcugggg     1440

ccuuggaggc agaaauggac ggagccaagg gucgacuaag cucaggccac cugaaaugcc     1500

gccucaagau ggauaagcug agacuuaaag gggugaguua uucccugugu accgcugccu     1560

ucacauuuac caagauacca gcagagacuu ugcauggaac gguuacagug gaaguacaau     1620

acgcgggcac cgacgguccc ugcaaagucc cugcucagau ggccguggac augcagacuc     1680

ucaccccagu ugggaggcug aucacagcaa accccgugau uaccgagagc acugaaaacu     1740

cuaagaugau guuagagcug gauccgccuu ucggcgacuc auacaucguc auaggagugg     1800

gcgagaaaaa gauuacacac cacuggcauc ggagugggag cacgaucgga aaggccuucg     1860

aagcuaccgu acgcggugcc aaaagaaugg cagugcuagg cgauacugcu ugggacuuug     1920

gguccguugg aggcgcccuc aauucucugg guaaggggau ccaccaaauu uucggcgcgg     1980

ccuuuaaauc gcuuuucgga ggcaugagcu gguuuuccca gaucuugauu gggacacugc     2040

ucauguggcu gggacuuaac accaagaaug gcucaauaag ucugaugugu uuggcucucg     2100

gugggguccu gaucuucuua ucuacagcag ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10445
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10445
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcguu acuaggcggg      120

gcuccgccua cuauauguac cuugaucgca acgacgcggg ggaggcuauu ucuuuuccca      180

cgacccuggg aaugaauaag ugcuauauac agaucaugga uuugggucau augugugacg      240

ccacaauguc auacgaaugc ccuauguuag augagggcgu ggaaccagac gauguagacu      300

guuggugcaa cacuaccagu acaugggugg ucuaugggac cugucaccau aaaaagggag      360

aggcacgacg uagcagaagg gccgugacuc ugccguccca cucuacacgg aaacuacaaa      420

cccgcucgca gacguggcuc gaaucaagag aguacacuaa gcaucugauu aggguugaga      480

auuggaucuu ccggaacccc ggcuuugcuc uugcagccgc ugccauugcg uggcuguugg      540

guagcaguac cucccagaaa gugaucuauc ucgucaugau acugcugauc gcacccgccu      600

acucuauucg cugcaucggg gugagcaauc gagauuucgu agaaggaaug uccggcggca      660

cauggguuga cauugugcuc gagcacgggg gaugugucac ugugauggcu caggauaagc      720

caaccgugga caucgaauua gucacaacca cgguuucaaa cauggccgag gugagaaguu      780

acugcuauga ggcaagcaua ucugauaugg cuuccgacuc aagguguccc acucaaggug      840

aagccuaccu ggacaagcag agcgauacac aguauguaug caaacggacc cuuguggacc      900

guggcugggg gaauggaugu ggcuuguuug guaaggggag ucuggucacu ugcgcgaaau      960

ucgccugcuc uaagaaaaug acaggaaagu ccauucagcc ugagaaccua gaauaccgca     1020

ucaugcucag cgugcacggc ucgcaacauu cuggcaugau uguuaaugau accgggcacg     1080

agacggacga aaacagagca aagguggaga ucaccccaaa uucccccagg gcugaggcca     1140

cacugggagg uuuuggcuca cuugggcugg auugugaacc ucggacugga uuggacuuca     1200

gugaucucua uuaccugacc augaacaaua aacauugguu aguccacaag gagugguuuc     1260

acgacauccc acugccgugg caugcuggcg ccgauacagg uacuccccac uggaacaaua     1320

aagaagcacu cguggaguuc aaggacgccc augcuaaacg acagaccgua guugugcuug     1380

ggagccagga aggcgcgguc cacacagcac uggccggagc uuuggaggcc gagauggaug     1440

gcgcaaaggg gcgccuaucc ucuggacauc ugaaaugcag acucaagaug gacaagcuga     1500

ggcuuaaagg ugugagcuau ucacugugua ccgccgcuuu uacuuucacg aagauuccug     1560

ccgaaacauu gcacggcacc gucacugugg agguucagua cgcagggacc gacggaccau     1620

gcaaagugcc cgcgcaaaug gcuguagaua ugcagacacu caccccuguc ggccggcuga     1680

uaacugccaa cccagugauc acagaaagua cggagaacuc caagaugaug uuagaacugg     1740

acccuccguu uggugauagc uacauuguua ucgggguggg cgagaaaaag auaacccacc     1800

auuggcaccg cucuggauca acuauuggca aggcauucga ggccacaguc agaggggcua     1860

aaaggauggc cgugcuagga gacaccgcau gggauuucgg uaguguaggc ggggcucuca     1920

auagccuggg aaagggcauc caucagaucu uuggugccgc guucaaaucc cuuuuugggg     1980

gcaugucuug guucucgcaa auuuugaucg gaacacugcu cauguggcug ggccuuaaca     2040

cuaagaaugg gagcauuucc cugauguguu uggcccucgg aggcgugcug auauuuuuau     2100

caaccgcugu uagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10446
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10446
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ucuguuccuc guggccgcug caacaagagu ccacagcguu acuaggcggg      120

gcuccgccua cuauauguac cuugaucgca acgacgcggg ggaggcuauu ucuuuuccca      180

cgacccuggg aaugaauaag ugcuauauac agaucaugga uuugggucau acaugugacg      240

ccacuauguc auacgaaugc ccuauguuag augagggcgu ggaaccagac gauguagacu      300

guuggugcaa caccacaagu accugggugg ucuaugggac uugucaccau aaaaagggag      360

aggcacgacg uagcagaagg gccgugacac ugccguccca cucuacccgg aaacuacaaa      420

cgcgcucgca gacuuggcuc gaaucaagag aguacaccaa gcaucugauu aggguugaga      480

auuggaucuu ccggaacccc ggcuuugcuc uugcagccgc ugccauugcg uggcuguugg      540

guagcaguac aucccagaaa gugaucuauc ucgucaugau acugcugauc gcacccgccu      600

acucuauucg cugcaucggg gugagcaauc gagauuucgu agaaggaaug uccggcggca      660

cuuggguuga cgugguccuc gagcacgggg gaugugugac cgugauggcu caggauaagc      720

caacagucga cauugaauua guuaccacga cugugucaaa cauggccgag guaagaaguu      780

acugcuauga ggcaagcauc ucugauaugg cuuccgacuc aagguguccc acacaaggug      840

aagccuaccu ggacaagcag agcgauaccc aguaugugug caaacggacu cuugucgacc      900

guggcugggg gaauggaugu ggcuuguuug guaaggggag ucuggugaca ugcgcgaaau      960

ucgccugcuc uaagaaaaug accggaaagu ccauacagcc ugagaaccua gaauaccgca     1020

uuaugcucag cguucacggc ucgcaacauu cuggcaugau cgugaaugau acggggcacg     1080

agaccgacga aaacagagca aaggucgaga uuacaccaaa uucccccagg gcugaggcca     1140

cucugggagg uuuuggcuca cuugggcugg auugugaacc ucggaccgga uuggacuuca     1200

gugaucucua uuaccugaca augaacaaua aacauugguu agugcacaag gagugguuuc     1260

acgacauccc acugccgugg caugcuggcg ccgauacugg uaccccucac uggaacaaua     1320

aagaagcacu cguagaguuc aaggacgccc augcuaaacg acagacaguu gugguccuug     1380

ggagccagga aggcgcggug cacaccgcac uggccggagc uuuggaggcc gagauggaug     1440

gcgcaaaggg gcgccuaucc ucuggacauc ugaaaugcag acucaagaug gacaagcuga     1500

ggcuuaaagg ugucagcuau ucacugugua cugccgcuuu uacguucaca aagaucccug     1560

ccgaaaccuu gcacggcacu gugaccguug aggugcagua cgcagggaca gacggaccau     1620

gcaaaguacc cgcgcaaaug gcugucgaua ugcagacccu cacuccugug ggccggcuga     1680

uuacagccaa cccaguuaua acggaaagua ccgagaacuc caagaugaug uuagaacugg     1740

aucccccguu uggugacagc uacaucguga uuggggucgg cgagaaaaag aucacucacc     1800

auuggcaccg cucuggauca acaauaggca aggcauucga ggccaccgug agaggggcua     1860

aaaggauggc cguacuagga gacacagcau gggauuucgg uagugugggc ggggcucuca     1920

auagccuggg aaagggcauu caucagaucu uuggugccgc guucaaaucc cuuuuugggg     1980

gcaugucuug guucucgcaa aucuugauug gaacucugcu cauguggcug ggccuuaacg     2040

ccaagaaugg gagcaucucc cugauguguu uggcucucgg aggcguucug aucuuuuuau     2100

caaccgcagu cagugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10447
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10447
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcauu acuaggcggg      120

gcuccgccua cuauauguac cuugaucgcu cugacgcggg gaaggcuaua ucauuugcca      180

cgacccuggg aguuaacaaa ugccaugugc agaucaugga uuugggucac augugugacg      240

caacaaugag uuaugagugc cccauguuag augaaggcgu agagccugac gauguggacu      300

guuggugcaa uacuaccagc acaugggucg uguacgggac cugucaucac aagaaaggag      360

aagcccgacg uuccagaagg gcuguuacuc ugccaucuca uucgacacgg aagcuacaaa      420

cccgcucaca gacguggcuc gagagcagag aauauacuaa acaccugauu aagguggaga      480

acuggaucuu caggaauccg ggcuuugcac uugucgccgu ggcuauugcc uggcuguugg      540

guaguuccac cucucagaag guaaucuacc ucguuaugau acugcugauc gcgcccgcau      600

auagcauucg gugcaucggg guguccaacc gcgauuucgu cgagggaaug ucaggcggca      660

caugggugga cgugguccuc gaacacgggg gauguguuac ugugauggcc caggauaaac      720

cuaccguaga cauugaguua gugacaacca cggucaguaa uauggcugaa gugcgaagcu      780

acugcuacga ggccucuauc uccgauaugg caucagacag cagaugucca acucaaggug      840

aggcuuaucu ggacaagcag agugauacac aguacguuug caaaaggacc cuuguggacc      900

ggggcugggg gaacggaugu ggcuuguuug guaagggguc ucuggucacu ugcgccaaau      960

ucacaugcuc caagaagaug accggaaaaa gcauacagcc cgaaaaucua gaguaucgua     1020

uuaugcucuc ggugcauggc ucucaacacu ccggcaugau cggguacgaa acggaugagg     1080

accgcgcgaa gguagaaguu accccuaacu caccaagagc cgaggcaaca cugggagguu     1140

uuggcagucu ugggcuggau ugugagccca ggacuggauu ggacuucagc gaucucuauu     1200

accugaccau gaauaacaaa cauugguuag ugcacaagga augguuucac gacauuccuc     1260

ugccauggca ugcuggcgcc gauacaggua cuccgcacug gaauaacaaa gaggcucucg     1320

ucgaguucaa ggacgcccau gcaaaacggc agaccguggu cgugcuuggg ucccaggaag     1380

gcgccguuca cacagcucug gcgggagcau uggaagccga gauggauggc gcuaaggggc     1440

gacuauuuuc uggacaucug aagugccgcc ucaaaaugga caagcugaga cuuaaaggug     1500

ugagcuauuc acuguguacc gccgcauuca cuuuuacgaa gguacccgcc gagacauugc     1560

acggcaccgu cacuguggaa guucaguacg cugggaccga cggaccuugc aaaaucccag     1620

ugcaaauggc cgucgauaug cagacacuca cccccguggg caggcugauc acugcaaauc     1680

cuguaauuac agagaguacg gaaaacucca agaugauguu agagcuggac ccacccuucg     1740

gugauagcua cauagugauc gggguuggcg acaagaaaau uacccaccau uggcaccggu     1800

cuggaucaac uaucggcaag gcguucgaag cuacaguccg cggggccaaa agaauggcag     1860

ugcuaggaga uaccgccugg gacuuuggua gugugggcgg ggucuucaau agccucggaa     1920

agggcauaca ucagauuuuu ggugcugccu ucaaaucccu guuugggggc augucuuggu     1980

ucucgcaaau ccuuaucgga acauugcugg uuuggcucgg ccugaacacu aagaacggga     2040

gcauuucccu uaccugucug gcauugggag gcgugaugau cuuucucuca acggcuguga     2100

gugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10448
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10448
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ucuguuccuc guggccgcug caacaagagu ccacagcguu acuaggcggg      120

gcuccgccua cuauauguac cuugaucgca acgacgcggg ggaggcuauu ucuuuuccca      180

cgacccuggg aaugaauaag ugcuauauac agaucaugga uuugggucau augugugacg      240

ccacaauguc auacgaaugc ccuauguuag augagggcgu ggaaccagac gauguagacu      300

guuggugcaa cacuaccagu acaugggugg ucuaugggac cugucaccau aaaaagggag      360

aggcacgacg uagcagaagg gccgugacuc ugccguccca cucuacacgg aaacuacaaa      420

cccgcucgca gacguggcuc gaaucaagag aguacacuaa gcaucugauu aggguugaga      480

auuggaucuu ccggaacccc ggcuuugcuc uugcagccgc ugccauugcg uggcuguugg      540

guagcaguac cucccagaaa gugaucuauc ucgucaugau acugcugauc gcacccgccu      600

acucuauucg cugcaucggg gugagcaauc gagauuucgu agaaggaaug uccggcggca      660

cauggguuga cgugguccuc gagcacgggg gaugugugac ugugauggcu caggauaagc      720

caaccgucga cauugaauua guuacaacca cggugucaaa cauggccgag guaagaaguu      780

acugcuauga ggcaagcauc ucugauaugg cuuccgacuc aagguguccc acucaaggug      840

aagccuaccu ggacaagcag agcgauacac aguaugugug caaacggacc cuugucgacc      900

guggcugggg gaauggaugu ggcuuguuug guaaggggag ucuggugacu ugcgcgaaau      960

ucgccugcuc uaagaaaaug acaggaaagu ccauacagcc ugagaaccua gaauaccgca     1020

uuaugcucag cguucacggc ucgcaacauu cuggcaugau cgugaaugau accgggcacg     1080

agacggacga aaacagagca aaggucgaga uuaccccaaa uucccccagg gcugaggcca     1140

cacugggagg uuuuggcuca cuugggcugg auugugaacc ucggacugga uuggacuuca     1200

gugaucucua uuaccugacc augaacaaua aacauugguu agugcacaag gagugguuuc     1260

acgacauccc acugccgugg caugcuggcg ccgauacagg uacuccccac uggaacaaua     1320

aagaagcacu cguagaguuc aaggacgccc augcuaaacg acagaccguu gugguccuug     1380

ggagccagga aggcgcggug cacacagcac uggccggagc uuuggaggcc gagauggaug     1440

gcgcaaaggg gcgccuaucc ucuggacauc ugaaaugcag acucaagaug gacaagcuga     1500

ggcuuaaagg ugucagcuau ucacugugua ccgccgcuuu uacuuucacg aagaucccug     1560

ccgaaacauu gcacggcacc gugacuguug aggugcagua cgcagggacc gacggaccau     1620

gcaaaguacc cgcgcaaaug gcugucgaua ugcagacacu caccccugug ggccggcuga     1680

uuacugccaa cccaguuaua acagaaagua cggagaacuc caagaugaug uuagaacugg     1740

acccuccguu uggugauagc uacaucguga uuggggucgg cgagaaaaag aucacccacc     1800

auuggcaccg cucuggauca acuauaggca aggcauucga ggccacagug agaggggcua     1860

aaaggauggc cguacuagga gacaccgcau gggauuucgg uagugugggc ggggcucuca     1920

auagccuggg aaagggcauu caucagaucu uuggugccgc guucaaaucc cuuuuugggg     1980

gcaugucuug guucucgcaa aucuugauug gaacacugcu cauguggcug ggccuuaaca     2040

cuaagaaugg gagcaucucc cugauguguu uggcccucgg aggcguucug aucuuuuuau     2100

caaccgcugu cagugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10449
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10449
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcuguuac caggcggggu ucagccuacu auauguaccu ugaccgcaac gaugcaggcg      180

aggccauaag uuuccccaca acucugggga ugaauaaaug uuauauccag auuauggacu      240

ugggacacau gugcgaugcu acgaugagcu acgaaugucc uauguuagac gagggcgugg      300

aaccagauga cguagauugc ugguguaaca ccacauccac uugggugguc uaugguaccu      360

gccaucacaa gaaaggggag gcacgacguu cuagaagggc cgugacacug ccgucgcauu      420

caacccggaa gcuacaaacu cgcagccaga cauggcucga aaguagagag uacaccaaac      480

accugaucag gguugagaau uggauuuuuc ggaaccccgg auucgcucuu gccgcggcag      540

ccaucgcuug gcuguugggc uccucuacga gccagaaggu gauauaucuc gucaugaucc      600

ugcugauugc cccugcauac uccauccgcu guauuggcgu gucaaaucga gacuuuguag      660

aagggaugag uggagguacu uggguugaua ucgugcucga gcauggcggg ugcgucaccg      720

ugauggcuca ggacaagcca acaguggaua uagaauuagu cacuaccaca guuagcaaca      780

uggccgaggu gagaucuuac uguuaugagg cguccauuuc agacauggcc agcgauagua      840

ggugccccac ccaaggagaa gcauaccugg acaaacaguc ugacacgcag uauguaugua      900

agcggacucu uguggaucgu ggcuggggua augggugcgg auuguucggc aaaggcuccc      960

uggucacaug cgcuaaguuu gccuguagca aaaagaugac cgggaagucg auccagccug     1020

agaaccuaga auaccgcauu augcucucug ugcacggauc ccaacacuca gguaugaucg     1080

uuaaugacac uggccaugag acagaugaaa acagagcuaa aguggagauc accccaaaua     1140

gucccagggc cgaggcaacg cuugggggau ucggcagccu gggucuggac ugcgaaccuc     1200

ggaccggguu ggauuuuucc gaccucuauu accugacaau gaacaauaag cacugguuag     1260

uccauaaaga gugguuccac gauauuccac ugccguggca cgccggcgcu gacacuggaa     1320

caccccauug gaacaauaag gaagcgcucg uggaguuuaa agaugcacac gccaagcgac     1380

agaccguagu ugugcuuggc ucucaggaag gggcugucca uacugcccug gcaggagccu     1440

uggaggcuga gauggacggu gccaaaggcc gccuaagcuc agggcaccug aaguguagac     1500

ucaagaugga uaaacugagg cuuaagggag ugaguuauuc ccugugcacc gcagcguuca     1560

cauuuaccaa aauaccugcu gaaacuuugc auggcacggu cacaguggag guucaguacg     1620

ccgguaccga cgggccaugu aaggugcccg cacaaauggc cguagacaug cagacucuca     1680

ccccugucgg ccggcugauc acagcuaacc cagugauuac cgaaagcacu gagaacucua     1740

aaaugauguu agaacuggau cccccguucg gagacucaua caucguuaua ggcguggggg     1800

agaagaagau uacacaccac uggcaucgca guggaagcac gaucgguaaa gccuuugagg     1860

caaccgucag aggcgcuaag aggauggccg ugcuagggga uacugcgugg gacuucggau     1920

ccguaggcgg ugcccucaau ucucugggga aaggcaucca ccagauuuuc ggagcugcau     1980

uuaagucgcu uuucggcggg augagcuggu uuucccaaau cuugauugga acacugcuca     2040

uguggcuggg ccuuaacacc aaaaaugguu caauaagucu gaugugcuug gcccucgggg     2100

gagugcugau cuucuuaucu acagcuguua gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10450
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10450
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcuguuac caggcggggu ucagccuacu auauguaccu ugaccgcaac gaugcaggcg      180

aggccauaag uuuccccaca acucugggga ugaauaaaug uuauauccag auuauggacu      240

ugggacacac gugcgaugcu accaugagcu acgaaugucc uauguuagac gagggcgugg      300

aaccagauga cguagauugc ugguguaaca caacuuccac cugggugguc uaugguacau      360

gccaucacaa gaaaggggag gcacgacguu cuagaagggc cgugacccug ccgucgcauu      420

caacucggaa gcuacaaaca cgcagccaga ccuggcucga aaguagagag uacacgaaac      480

accugaucag gguugagaau uggauuuuuc ggaaccccgg auucgcucuu gccgcggcag      540

ccaucgcuug gcuguugggc uccucuacua gccagaaggu gauauaucuc gucaugaucc      600

ugcugauugc cccugcauac uccauccgcu guauuggcgu gucaaaucga gacuuuguag      660

aagggaugag uggagguacc uggguugaug ugguccucga gcauggcggg ugcgugacag      720

ugauggcuca ggacaagcca acugucgaua ucgaauuagu uaccacaacc gugagcaaca      780

uggccgaggu aagaucuuac uguuaugagg cguccauauc agacauggcc agcgauagua      840

ggugccccac gcaaggagaa gcauaccugg acaaacaguc ugacacucag uaugugugua      900

agcggacacu ugucgaucgu ggcuggggua augggugcgg auuguucggc aaaggcuccc      960

uggugaccug cgcuaaguuu gccuguagca aaaagaugac ugggaagucg auucagccug     1020

agaaccuaga auaccgcauc augcucucug uucacggauc ccaacacuca gguaugauug     1080

ugaaugacac aggccaugag accgaugaaa acagagcuaa agucgagauc acgccaaaua     1140

gucccagggc cgaggcaacc cuugggggau ucggcagccu gggucuggac ugcgaaccuc     1200

ggacaggguu ggauuuuucc gaccucuauu accugacuau gaacaauaag cacugguuag     1260

ugcauaaaga gugguuccac gauaucccac ugccguggca cgccggcgcu gacaccggaa     1320

caccccauug gaacaauaag gaagcgcucg uagaguuuaa agaugcacac gccaagcgac     1380

agacuguugu gguccuuggc ucucaggaag gggcugugca uaccgcccug gcaggagccu     1440

uggaggcuga gauggacggu gccaaaggcc gccuaagcuc agggcaccug aaguguagac     1500

ucaagaugga uaaacugagg cuuaagggag ucaguuauuc ccugugcaca gcagcguuca     1560

ccuuuacuaa aauuccugcu gaaacguugc auggcacagu gaccguugag gugcaguacg     1620

ccgguacuga cgggccaugu aagguacccg cacaaauggc cgucgacaug cagacccuca     1680

caccuguggg ccggcugaua accgcuaacc caguuaucac ugaaagcaca gagaacucua     1740

aaaugauguu agaacuggau cccccguucg gagacucaua cauugugauc ggcgucgggg     1800

agaagaagau aacgcaccac uggcaucgca guggaagcac cauugguaaa gccuuugagg     1860

caacugugag aggcgcuaag aggauggccg uacuagggga uacagcgugg gacuucggau     1920

ccgugggcgg ugcccucaau ucucugggga aaggcaucca ccagaucuuc ggagcugcau     1980

uuaagucgcu uuucggcggg augagcuggu uuucccaaau uuugaucgga acccugcuca     2040

uguggcuggg ccuuaacgcc aaaaaugguu caauuagucu gaugugcuug gcucucgggg     2100

gaguucugau auucuuaucu acagccguca gcgcaugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10451
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10451
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcuauaac caggcggggu ucagccuacu auauguaccu ugaccgcagu gaugcaggca      180

aagccaucag cuucgcuaca acucuggggg uuaacaagug ucacgugcag auuauggacu      240

ugggacauau gugcgaugca acgauguccu augagugucc cauguuagac gaaggcguag      300

agccugauga cguggauugc ugguguaaua ccacaucuac uugggucgug uacgguaccu      360

gccaccauaa aaagggggaa gcccgacguu cgagaagggc uguuacacug ccaucacaca      420

gcacccggaa acuacaaacu cgcagucaga cauggcucga guccagagaa uauaccaagc      480

aucugaucaa gguggagaac uggauuuuua ggaauccggg auucgcccuu gucgcggugg      540

caaucgccug gcuguugggc ucuagcacgu cccagaaagu aauauaccuc guuaugaucc      600

ugcugauugc ucccgccuau ucaauccggu guauuggcgu gaguaaccgc gacuuugucg      660

aggggaugag cggagguacu uggguggaug ugguccucga acacggcggg ugcguuaccg      720

ugauggcaca ggacaagccu acaguagaua ucgaguuagu gacuaccaca gucucuaaua      780

uggcugaagu gcgauccuac uguuacgagg ccucaauaag cgacauggcg agugauucua      840

gaugcccaac ccaaggagag gccuaucugg acaaacaguc cgacacgcag uacguuugua      900

agaggacucu uguggaucgg ggcuggggua acgggugcgg auuguucggc aaaggcagcc      960

uggucacaug cgcaaaguuu accuguucga agaaaaugac ugggaagucu auucagcccg     1020

aaaaucuaga guaucguauc augcucuccg ugcacggauc acaacauagu gguaugauug     1080

gcuacgaaac agacgaggau cgcgcuaaag uagaaguuac cccuaacagc ccaagagccg     1140

aggcuacgcu gggaggguuc ggcucccuug gucuggacug cgagcccagg accggguugg     1200

auuuuucuga ccucuauuac cugacaauga auaacaagca cugguuagug cauaaagaau     1260

gguuccacga uaucccucug ccauggcacg ccggcgcaga cacuggaacc ccgcauugga     1320

auaacaagga ggcccucguc gaauuuaaag augcucacgc gaagcggcag acaguggucg     1380

ugcuuggcag ccaggagggg gcaguucaua cugcccuggc uggagccuug gaagcagaga     1440

uggacggugc caagggccga cuauucucag ggcaccugaa augucgccuc aagauggaua     1500

aacugagacu uaagggagug aguuauuccc ugugcaccgc ugccuuuaca uucaccaaag     1560

uacccgcaga gacuuugcau ggcacgguca caguggaagu ucaguacgcg gguaccgacg     1620

ggccuuguaa gaucccagug caaauggcug ucgacaugca gacucucacc cccgugggca     1680

ggcugauuac agccaauccu guaauaaccg agagcacuga aaacucuaag augauguuag     1740

agcuggaucc acccuuugga gacucauaca ucgugauugg cguuggggau aaaaagauca     1800

cacaccacug gcaucggagu ggaagcacga uagguaaagc auucgaagcc accguccgcg     1860

gcgcuaagag aauggccgug cuaggggaca cugcauggga uuucggaucc gugggcggug     1920

ucuuuaauuc ucucgggaaa ggcauucacc agaucuucgg agcugccuuu aagucgcugu     1980

ucggcgggau gagcugguuu ucccaaaucc uuauuggaac auugcugguu uggcucggcc     2040

ugaacaccaa gaacgguuca aucagucuua caugccuggc gcucggggga gugaugauuu     2100

ucuugucuac ugccgugagc gcuugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10452
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10452
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcuguuac caggcggggu ucagccuacu auauguaccu ugaccgcaac gaugcaggcg      180

aggccauaag uuuccccaca acucugggga ugaauaaaug uuauauccag auuauggacu      240

ugggacacau gugcgaugcu acgaugagcu acgaaugucc uauguuagac gagggcgugg      300

aaccagauga cguagauugc ugguguaaca ccacauccac uugggugguc uaugguaccu      360

gccaucacaa gaaaggggag gcacgacguu cuagaagggc cgugacacug ccgucgcauu      420

caacccggaa gcuacaaacu cgcagccaga cauggcucga aaguagagag uacaccaaac      480

accugaucag gguugagaau uggauuuuuc ggaaccccgg auucgcucuu gccgcggcag      540

ccaucgcuug gcuguugggc uccucuacga gccagaaggu gauauaucuc gucaugaucc      600

ugcugauugc cccugcauac uccauccgcu guauuggcgu gucaaaucga gacuuuguag      660

aagggaugag uggagguacu uggguugaug ugguccucga gcauggcggg ugcgugaccg      720

ugauggcuca ggacaagcca acagucgaua ucgaauuagu uacuaccaca gugagcaaca      780

uggccgaggu aagaucuuac uguuaugagg cguccauauc agacauggcc agcgauagua      840

ggugccccac ccaaggagaa gcauaccugg acaaacaguc ugacacgcag uaugugugua      900

agcggacucu ugucgaucgu ggcuggggua augggugcgg auuguucggc aaaggcuccc      960

uggugacaug cgcuaaguuu gccuguagca aaaagaugac cgggaagucg auucagccug     1020

agaaccuaga auaccgcauc augcucucug uucacggauc ccaacacuca gguaugauug     1080

ugaaugacac uggccaugag acagaugaaa acagagcuaa agucgagauc accccaaaua     1140

gucccagggc cgaggcaacg cuugggggau ucggcagccu gggucuggac ugcgaaccuc     1200

ggaccggguu ggauuuuucc gaccucuauu accugacaau gaacaauaag cacugguuag     1260

ugcauaaaga gugguuccac gauaucccac ugccguggca cgccggcgcu gacacuggaa     1320

caccccauug gaacaauaag gaagcgcucg uagaguuuaa agaugcacac gccaagcgac     1380

agaccguugu gguccuuggc ucucaggaag gggcugugca uacugcccug gcaggagccu     1440

uggaggcuga gauggacggu gccaaaggcc gccuaagcuc agggcaccug aaguguagac     1500

ucaagaugga uaaacugagg cuuaagggag ucaguuauuc ccugugcacc gcagcguuca     1560

cauuuaccaa aauuccugcu gaaacuuugc auggcacggu gacaguugag gugcaguacg     1620

ccgguaccga cgggccaugu aagguacccg cacaaauggc cgucgacaug cagacucuca     1680

ccccuguggg ccggcugaua acagcuaacc caguuaucac cgaaagcacu gagaacucua     1740

aaaugauguu agaacuggau cccccguucg gagacucaua cauugugauc ggcgucgggg     1800

agaagaagau aacacaccac uggcaucgca guggaagcac gauugguaaa gccuuugagg     1860

caaccgugag aggcgcuaag aggauggccg uacuagggga uacugcgugg gacuucggau     1920

ccgugggcgg ugcccucaau ucucugggga aaggcaucca ccagaucuuc ggagcugcau     1980

uuaagucgcu uuucggcggg augagcuggu uuucccaaau uuugaucgga acacugcuca     2040

uguggcuggg ccuuaacacc aaaaaugguu caauuagucu gaugugcuug gcccucgggg     2100

gaguucugau auucuuaucu acagcuguca gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10453
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10453
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgu gaccagaagg ggaucagcgu acuauaugua ccuugaccgg aacgaugcug      180

gugaagccau caguuucccu acaacucugg gcaugaauaa gugcuauauu caaaucaugg      240

acuuggggca caugugugau gcaacgauga gcuacgagug cccaauguua gacgaaggag      300

uagagccgga ugacguggau uguuggugca acaccacauc cacuuggguc guguauggca      360

ccugucauca caaaaagggu gaagcccgcc gaucucguag agcuguuaca cugcccucgc      420

auucaaccag gaaacuacag acucggagcc agacauggcu cgagagucgc gaguacacca      480

agcaccugau uagaguggaa aauuggaucu uuaggaaccc uggguucgca cuugccgcug      540

ccgcgauagc auggcuguug ggauccucua cgagccagaa agucaucuau cucgugauga      600

uucugcugau cgccccagcu uacuccauuc ggugcaucgg cguaucaaau cgcgacuuug      660

uugagggcau gaguggggga acuugggugg auauaguccu cgaacauggu ggcuguguga      720

ccgugauggc ccaagacaag cccacagucg auauugaguu aguuacuacc acagugagca      780

acauggcaga gguacgaucu uacugcuaug aggcuuccau cucagacaug gccagcgaua      840

guagaugucc uacccagggg gaagcguacc uggacaagca gucugacacg caguaugugu      900

gcaaaaggac ucuugucgau cggggauggg gcaaugguug uggguuguuc ggaaagggcu      960

cccuggugac augcgccaaa uuugcaugca gcaagaaaau gaccggcaag ucgauucaac     1020

cagaaaaccu agaguaccgu aucaugcucu cuguucacgg gucccagcac ucaggaauga     1080

ucgugaauga cacuggucau gaaacagaug agaaccgcgc uaaggucgag auuaccccca     1140

auaguccuag agccgaagcu acgcugggcg gguucggaag ccuuggccug gacugugagc     1200

caaggaccgg uuuggauuuu uccgaccucu auuaccugac aaugaacaau aaacacuggu     1260

uagugcauaa ggaaugguuc cacgauauac cgcugcccug gcacgccggg gcagacacug     1320

gcaccccuca uuggaacaau aaagaggccc ucguagaauu uaaggaugcu cacgcgaaac     1380

ggcagacagu ugugguccuu ggaucucagg agggcgcagu gcauacugcc cuggcugggg     1440

ccuuggaggc agaaauggac ggagccaagg gucgacuaag cucaggccac cugaaaugcc     1500

gccucaagau ggauaagcug agacuuaaag gggucaguua uucccugugu accgcugccu     1560

ucacauuuac caagauccca gcagagacuu ugcauggaac ggugacaguu gaagugcaau     1620

acgcgggcac cgacgguccc ugcaaaguac cugcucagau ggccgucgac augcagacuc     1680

ucaccccagu ggggaggcug auuacagcaa accccguuau caccgagagc acugaaaacu     1740

cuaagaugau guuagagcug gauccgccuu ucggcgacuc auacauagug auuggagucg     1800

gcgagaaaaa gaucacacac cacuggcauc ggagugggag cacgcuagga aaggccuucu     1860

ccaccacucu caaaggugcu caacgccugg ccgcacuugg cgauacagcu ugggacuuug     1920

ggucuaucgg aggcguguuc aauucgauug guagagccgu acaccaggug uuugggggcg     1980

cguucaggac cuuguuugga ggcaugagcu ggaucacaca ggggcugaug ggagcccucc     2040

ugcuuuggau gggcguuaac gcucgugauc gguccauugc acuggccuuc uuggcuacug     2100

gugggguccu cguguuucug gccaccaaug ugcaugcaug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10454
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10454
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgu gaccagaagg ggaucagcgu acuauaugua ccuugaccgg aacgaugcug      180

gugaagccau caguuucccu acaacucugg gcaugaauaa gugcuauauu caaaucaugg      240

acuuggggca cacgugugau gcaaccauga gcuacgagug cccaauguua gacgaaggag      300

uagagccgga ugacguggau uguuggugca acacaacuuc caccuggguc guguauggca      360

caugucauca caaaaagggu gaagcccgcc gaucucguag agcuguuacc cugcccucgc      420

auucaacuag gaaacuacag acacggagcc agaccuggcu cgagagucgc gaguacacga      480

agcaccugau uagaguggaa aauuggaucu uuaggaaccc uggguucgca cuugccgcug      540

ccgcgauagc auggcuguug ggauccucua cuagccagaa agucaucuau cucgugauga      600

uucugcugau cgccccagcu uacuccauuc ggugcaucgg cguaucaaau cgcgacuuug      660

uugagggcau gaguggggga accugggugg augucgugcu cgaacauggu ggcuguguga      720

cagucauggc ccaagacaag cccacuguug auauagaguu agugaccaca accguaagca      780

acauggcaga ggugcgaucu uacugcuaug aggcuuccau uucagacaug gccagcgaua      840

guagaugucc uacgcagggg gaagcguacc uggacaagca gucugacacu caguaugucu      900

gcaaaaggac acuuguggau cggggauggg gcaaugguug uggguuguuc ggaaagggcu      960

cccugguuac cugcgccaaa uuugcaugca gcaagaaaau gacuggcaag ucgauccaac     1020

cagaaaaccu agaguaccgu auuaugcucu cugugcacgg gucccagcac ucaggaauga     1080

ucgucaauga cacaggucau gaaaccgaug agaaccgcgc uaagguggag aucacgccca     1140

auaguccuag agccgaagcu acccugggcg gguucggaag ccuuggccug gacugugagc     1200

caaggacagg uuuggauuuu uccgaccucu auuaccugac uaugaacaau aaacacuggu     1260

uaguacauaa ggaaugguuc cacgauauuc cgcugcccug gcacgccggg gcagacaccg     1320

gcacaccuca uuggaacaau aaagaggccc ucguugaauu uaaggaugcu cacgcgaaac     1380

ggcagacugu ggucgugcuu ggaucucagg agggcgcagu ccauaccgcc cuggcugggg     1440

ccuuggaggc agaaauggac ggagccaagg gucgacuaag cucaggccac cugaaaugcc     1500

gccucaagau ggauaagcug agacuuaaag gggugaguua uucccugugu acagcugccu     1560

ucaccuuuac uaagauacca gcagagacgu ugcauggaac aguuaccgug gaaguacaau     1620

acgcgggcac ugacgguccc ugcaaagucc cugcucagau ggccguggac augcagaccc     1680

ucacaccagu ugggaggcug aucaccgcaa accccgugau uacugagagc acagaaaacu     1740

cuaagaugau guuagagcug gauccgccuu ucggcgacuc auacaucguc auaggagugg     1800

gcgagaaaaa gauuacgcac cacuggcauc ggagugggag cacccuagga aaggccuucu     1860

ccacuacacu caaaggugcu caacgccugg ccgcacuugg cgauaccgcu ugggacuuug     1920

ggucuaucgg aggcguauuc aauucgaucg guagagccgu gcaccagguu uuugggggcg     1980

cguucaggac auuguuugga ggcaugagcu ggauuacuca ggggcugaug ggagcccucc     2040

ugcuuuggau gggcgucaac gcucgugauc gguccaucgc acuggccuuc uuggcuaccg     2100

guggggugcu cguguuucug gccacgaaug uccaugcaug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10455
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10455
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccau caccagaagg ggaucagcgu acuauaugua ccuugaccgg agugaugcug      180

guaaggccau uagcuucgca acaacucugg gcgugaacaa augccacgua caaaucaugg      240

acuuggggca uaugugugau gccacgaugu ccuaugaaug cccuauguua gacgagggag      300

uggaaccaga ugacgucgau uguuggugca auaccacauc uacuugggug guuuacggca      360

ccugucacca uaagaaaggu gaggcucgcc gaucgcguag agcagugaca cugccgucac      420

acagcaccag gaagcuacag acucggaguc agacauggcu cgaaucccgc gaguauacca      480

aacaucugau uaaggucgag aacuggaucu uuagaaaucc cggguucgcc cuuguggcug      540

uagccauagc guggcuguug ggaucuagca cgucccagaa gguuaucuac cucgugauga      600

uucugcugau cgcacccgcc uauucaauua ggugcaucgg cgucaguaac cgggacuuug      660

uggaaggcau gagcggggga acuugggugg augucguucu cgagcacggu ggcuguguga      720

ccguaauggc ucaagacaaa ccaacagugg auauagaauu agucacuacc acagugucua      780

auauggccga gguucgcucc uacugcuacg aggcaucaau uagcgacaug gcuagugauu      840

cucgaugucc cacccagggg gaagccuauc uggacaagca guccgacacg caguacgugu      900

gcaaaagaac ucuugucgau aggggauggg gcaacgguug uggguuguuc ggaaagggca      960

gccuggugac augcgcgaaa uuuaccugcu cgaagaagau gacuggcaaa ucuauccaac     1020

cugagaaucu agaauaucgg auuaugcucu ccguacacgg gucacagcau aguggaauga     1080

ucgguuacga gacagacgaa gaucgugcca agguugaggu gaccccaaac agcccccgcg     1140

cagaggcuac gcugggcggg uucggauccc uuggccugga cugugaaccu agaaccgguu     1200

uggauuuuuc ugaccucuau uaccugacaa ugaauaacaa acacugguua guccauaagg     1260

agugguucca cgauauccca cugccguggc acgccggggc ugacacuggc accccucauu     1320

ggaauaacaa agaagcccuc guggaguuua aggaugcaca cgccaaaagg cagacagucg     1380

ugguucuugg aagccaggaa ggcgcugugc auacugcgcu ggcaggggcc uuggaggcug     1440

agauggacgg agccaagggu cggcuauucu caggccaccu gaagugccga cucaaaaugg     1500

auaagcugcg ccuuaaaggg guaaguuauu cccuguguac cgcagccuuu acauucacca     1560

aggucccugc ugaaacuuug cauggaacgg ugacaguuga ggugcaauac gccggcaccg     1620

acgguccaug caaaauuccc guccagaugg caguggacau gcagacucuc accccuguag     1680

ggagacugau aacagcgaau ccagugauca ccgaaagcac ugagaacucu aagaugaugu     1740

uagaacugga ucccccguuc ggcgacucau acauuguuau cggagucggc gauaagaaaa     1800

uaacacacca cuggcauagg agugggagca cgcuaggaaa ggcuuuuucc accacucuca     1860

aaggugccca acggcuggca gcccuuggcg acacagcuug ggauuucggg ucuauuggag     1920

gcguguuuaa uucgaucggu cgcgccgugc accaggucuu cgggggcgca uuuagaaccu     1980

uguucggagg caugagcugg aucacacagg ggcugauggg agcucuccug cuuuggaugg     2040

gcguuaacgc cagggaccgu uccauugcgc uggccuuuuu ggcuacuggu ggggugcucg     2100

uguuccuggc aaccaacgua caugccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10456
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10456
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgu gaccagaagg ggaucagcgu acuauaugua ccuugaccgg aacgaugcug      180

gugaagccau caguuucccu acaacucugg gcaugaauaa gugcuauauu caaaucaugg      240

acuuggggca caugugugau gcaacgauga gcuacgagug cccaauguua gacgaaggag      300

uagagccgga ugacguggau uguuggugca acaccacauc cacuuggguc guguauggca      360

ccugucauca caaaaagggu gaagcccgcc gaucucguag agcuguuaca cugcccucgc      420

auucaaccag gaaacuacag acucggagcc agacauggcu cgagagucgc gaguacacca      480

agcaccugau uagaguggaa aauuggaucu uuaggaaccc uggguucgca cuugccgcug      540

ccgcgauagc auggcuguug ggauccucua cgagccagaa agucaucuau cucgugauga      600

uucugcugau cgccccagcu uacuccauuc ggugcaucgg cguaucaaau cgcgacuuug      660

uugagggcau gaguggggga acuugggugg augucgugcu cgaacauggu ggcuguguga      720

ccgucauggc ccaagacaag cccacaguug auauagaguu agugacuacc acaguaagca      780

acauggcaga ggugcgaucu uacugcuaug aggcuuccau uucagacaug gccagcgaua      840

guagaugucc uacccagggg gaagcguacc uggacaagca gucugacacg caguaugucu      900

gcaaaaggac ucuuguggau cggggauggg gcaaugguug uggguuguuc ggaaagggcu      960

cccugguuac augcgccaaa uuugcaugca gcaagaaaau gaccggcaag ucgauccaac     1020

cagaaaaccu agaguaccgu auuaugcucu cugugcacgg gucccagcac ucaggaauga     1080

ucgucaauga cacuggucau gaaacagaug agaaccgcgc uaagguggag aucaccccca     1140

auaguccuag agccgaagcu acgcugggcg gguucggaag ccuuggccug gacugugagc     1200

caaggaccgg uuuggauuuu uccgaccucu auuaccugac aaugaacaau aaacacuggu     1260

uaguacauaa ggaaugguuc cacgauauuc cgcugcccug gcacgccggg gcagacacug     1320

gcaccccuca uuggaacaau aaagaggccc ucguugaauu uaaggaugcu cacgcgaaac     1380

ggcagacagu ggucgugcuu ggaucucagg agggcgcagu ccauacugcc cuggcugggg     1440

ccuuggaggc agaaauggac ggagccaagg gucgacuaag cucaggccac cugaaaugcc     1500

gccucaagau ggauaagcug agacuuaaag gggugaguua uucccugugu accgcugccu     1560

ucacauuuac caagauacca gcagagacuu ugcauggaac gguuacagug gaaguacaau     1620

acgcgggcac cgacgguccc ugcaaagucc cugcucagau ggccguggac augcagacuc     1680

ucaccccagu ugggaggcug aucacagcaa accccgugau uaccgagagc acugaaaacu     1740

cuaagaugau guuagagcug gauccgccuu ucggcgacuc auacaucguc auaggagugg     1800

gcgagaaaaa gauuacacac cacuggcauc ggagugggag cacgcuagga aaggccuucu     1860

ccaccacucu caaaggugcu caacgccugg ccgcacuugg cgauacagcu ugggacuuug     1920

ggucuaucgg aggcguauuc aauucgaucg guagagccgu gcaccagguu uuugggggcg     1980

cguucaggac cuuguuugga ggcaugagcu ggauuacaca ggggcugaug ggagcccucc     2040

ugcuuuggau gggcgucaac gcucgugauc gguccaucgc acuggccuuc uuggcuacug     2100

guggggugcu cguguuucug gccaccaaug uccaugcaug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10457
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10457
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcguu acuaggcggg      120

gcuccgccua cuauauguac cuugaucgca acgacgcggg ggaggcuauu ucuuuuccca      180

cgacccuggg aaugaauaag ugcuauauac agaucaugga uuugggucau augugugacg      240

ccacaauguc auacgaaugc ccuauguuag augagggcgu ggaaccagac gauguagacu      300

guuggugcaa cacuaccagu acaugggugg ucuaugggac cugucaccau aaaaagggag      360

aggcacgacg uagcagaagg gccgugacuc ugccguccca cucuacacgg aaacuacaaa      420

cccgcucgca gacguggcuc gaaucaagag aguacacuaa gcaucugauu aggguugaga      480

auuggaucuu ccggaacccc ggcuuugcuc uugcagccgc ugccauugcg uggcuguugg      540

guagcaguac cucccagaaa gugaucuauc ucgucaugau acugcugauc gcacccgccu      600

acucuauucg cugcaucggg gugagcaauc gagauuucgu agaaggaaug uccggcggca      660

cauggguuga cauugugcuc gagcacgggg gaugugucac ugugauggcu caggauaagc      720

caaccgugga caucgaauua gucacaacca cgguuucaaa cauggccgag gugagaaguu      780

acugcuauga ggcaagcaua ucugauaugg cuuccgacuc aagguguccc acucaaggug      840

aagccuaccu ggacaagcag agcgauacac aguauguaug caaacggacc cuuguggacc      900

guggcugggg gaauggaugu ggcuuguuug guaaggggag ucuggucacu ugcgcgaaau      960

ucgccugcuc uaagaaaaug acaggaaagu ccauucagcc ugagaaccua gaauaccgca     1020

ucaugcucag cgugcacggc ucgcaacauu cuggcaugau uguuaaugau accgggcacg     1080

agacggacga aaacagagca aagguggaga ucaccccaaa uucccccagg gcugaggcca     1140

cacugggagg uuuuggcuca cuugggcugg auugugaacc ucggacugga uuggacuuca     1200

gugaucucua uuaccugacc augaacaaua aacauugguu aguccacaag gagugguuuc     1260

acgacauccc acugccgugg caugcuggcg ccgauacagg uacuccccac uggaacaaua     1320

aagaagcacu cguggaguuc aaggacgccc augcuaaacg acagaccgua guugugcuug     1380

ggagccagga aggcgcgguc cacacagcac uggccggagc uuuggaggcc gagauggaug     1440

gcgcaaaggg gcgccuaucc ucuggacauc ugaaaugcag acucaagaug gacaagcuga     1500

ggcuuaaagg ugugagcuau ucacugugua ccgccgcuuu uacuuucacg aagauuccug     1560

ccgaaacauu gcacggcacc gucacugugg agguucagua cgcagggacc gacggaccau     1620

gcaaagugcc cgcgcaaaug gcuguagaua ugcagacacu caccccuguc ggccggcuga     1680

uaacugccaa cccagugauc acagaaagua cggagaacuc caagaugaug uuagaacugg     1740

acccuccguu uggugauagc uacauuguua ucgggguggg cgagaaaaag auaacccacc     1800

auuggcaccg cucuggauca acucuaggca aggcauucag uacaacccuc aaaggggccc     1860

agagacuggc ugcccuugga gacacagcau gggauuucgg uagcauuggc ggggucuuua     1920

auuccaucgg aagggcugug caucaaguau ucggcggugc cuuucguacu uuguucgggg     1980

gcaugucuug gaucacccag ggacugaugg gcgcgcuccu gcuuuggaug ggggugaacg     2040

cccgggaccg aucgauugcu cuggcauuuu uggccacggg aggcguucuc gucuuccugg     2100

cuaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10458
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10458
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcguu acuaggcggg      120

gcuccgccua cuauauguac cuugaucgca acgacgcggg ggaggcuauu ucuuuuccca      180

cgacccuggg aaugaauaag ugcuauauac agaucaugga uuugggucau acaugugacg      240

ccacuauguc auacgaaugc ccuauguuag augagggcgu ggaaccagac gauguagacu      300

guuggugcaa caccacaagu accugggugg ucuaugggac uugucaccau aaaaagggag      360

aggcacgacg uagcagaagg gccgugacac ugccguccca cucuacccgg aaacuacaaa      420

cgcgcucgca gacuuggcuc gaaucaagag aguacaccaa gcaucugauu aggguugaga      480

auuggaucuu ccggaacccc ggcuuugcuc uugcagccgc ugccauugcg uggcuguugg      540

guagcaguac aucccagaaa gugaucuauc ucgucaugau acugcugauc gcacccgccu      600

acucuauucg cugcaucggg gugagcaauc gagauuucgu agaaggaaug uccggcggca      660

cuuggguuga cgugguccuc gagcacgggg gaugugugac cgugauggcu caggauaagc      720

caacagucga cauugaauua guuaccacga cugugucaaa cauggccgag guaagaaguu      780

acugcuauga ggcaagcauc ucugauaugg cuuccgacuc aagguguccc acacaaggug      840

aagccuaccu ggacaagcag agcgauaccc aguaugugug caaacggacu cuugucgacc      900

guggcugggg gaauggaugu ggcuuguuug guaaggggag ucuggugaca ugcgcgaaau      960

ucgccugcuc uaagaaaaug accggaaagu ccauacagcc ugagaaccua gaauaccgca     1020

uuaugcucag cguucacggc ucgcaacauu cuggcaugau cgugaaugau acggggcacg     1080

agaccgacga aaacagagca aaggucgaga uuacaccaaa uucccccagg gcugaggcca     1140

cucugggagg uuuuggcuca cuugggcugg auugugaacc ucggaccgga uuggacuuca     1200

gugaucucua uuaccugaca augaacaaua aacauugguu agugcacaag gagugguuuc     1260

acgacauccc acugccgugg caugcuggcg ccgauacugg uaccccucac uggaacaaua     1320

aagaagcacu cguagaguuc aaggacgccc augcuaaacg acagacaguu gugguccuug     1380

ggagccagga aggcgcggug cacaccgcac uggccggagc uuuggaggcc gagauggaug     1440

gcgcaaaggg gcgccuaucc ucuggacauc ugaaaugcag acucaagaug gacaagcuga     1500

ggcuuaaagg ugucagcuau ucacugugua cugccgcuuu uacguucaca aagaucccug     1560

ccgaaaccuu gcacggcacu gugaccguug aggugcagua cgcagggaca gacggaccau     1620

gcaaaguacc cgcgcaaaug gcugucgaua ugcagacccu cacuccugug ggccggcuga     1680

uuacagccaa cccaguuaua acggaaagua ccgagaacuc caagaugaug uuagaacugg     1740

aucccccguu uggugacagc uacaucguga uuggggucgg cgagaaaaag aucacucacc     1800

auuggcaccg cucuggauca acacuaggca aggcauucag uaccacacuc aaaggggccc     1860

agagacuggc ugcccuugga gacacugcau gggauuucgg uagcauaggc gggguguuua     1920

auuccauugg aagggcugua caucaagugu ucggcggugc cuuucguacc uuguucgggg     1980

gcaugucuug gaucacgcag ggacugaugg gcgcgcuccu gcuuuggaug gggguuaacg     2040

cccgggaccg aucgaucgcu cuggcauuuu uggccaccgg aggcguccuc guguuccugg     2100

cuacaaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10459
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10459
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcauu acuaggcggg      120

gcuccgccua cuauauguac cuugaucgcu cugacgcggg gaaggcuaua ucauuugcca      180

cgacccuggg aguuaacaaa ugccaugugc agaucaugga uuugggucac augugugacg      240

caacaaugag uuaugagugc cccauguuag augaaggcgu agagccugac gauguggacu      300

guuggugcaa uacuaccagc acaugggucg uguacgggac cugucaucac aagaaaggag      360

aagcccgacg uuccagaagg gcuguuacuc ugccaucuca uucgacacgg aagcuacaaa      420

cccgcucaca gacguggcuc gagagcagag aauauacuaa acaccugauu aagguggaga      480

acuggaucuu caggaauccg ggcuuugcac uugucgccgu ggcuauugcc uggcuguugg      540

guaguuccac cucucagaag guaaucuacc ucguuaugau acugcugauc gcgcccgcau      600

auagcauucg gugcaucggg guguccaacc gcgauuucgu cgagggaaug ucaggcggca      660

caugggugga cgugguccuc gaacacgggg gauguguuac ugugauggcc caggauaaac      720

cuaccguaga cauugaguua gugacaacca cggucaguaa uauggcugaa gugcgaagcu      780

acugcuacga ggccucuauc uccgauaugg caucagacag cagaugucca acucaaggug      840

aggcuuaucu ggacaagcag agugauacac aguacguuug caaaaggacc cuuguggacc      900

ggggcugggg gaacggaugu ggcuuguuug guaagggguc ucuggucacu ugcgccaaau      960

ucacaugcuc caagaagaug accggaaaaa gcauacagcc cgaaaaucua gaguaucgua     1020

uuaugcucuc ggugcauggc ucucaacacu ccggcaugau cggguacgaa acggaugagg     1080

accgcgcgaa gguagaaguu accccuaacu caccaagagc cgaggcaaca cugggagguu     1140

uuggcagucu ugggcuggau ugugagccca ggacuggauu ggacuucagc gaucucuauu     1200

accugaccau gaauaacaaa cauugguuag ugcacaagga augguuucac gacauuccuc     1260

ugccauggca ugcuggcgcc gauacaggua cuccgcacug gaauaacaaa gaggcucucg     1320

ucgaguucaa ggacgcccau gcaaaacggc agaccguggu cgugcuuggg ucccaggaag     1380

gcgccguuca cacagcucug gcgggagcau uggaagccga gauggauggc gcuaaggggc     1440

gacuauuuuc uggacaucug aagugccgcc ucaaaaugga caagcugaga cuuaaaggug     1500

ugagcuauuc acuguguacc gccgcauuca cuuuuacgaa gguacccgcc gagacauugc     1560

acggcaccgu cacuguggaa guucaguacg cugggaccga cggaccuugc aaaaucccag     1620

ugcaaauggc cgucgauaug cagacacuca cccccguggg caggcugauc acugcaaauc     1680

cuguaauuac agagaguacg gaaaacucca agaugauguu agagcuggac ccacccuucg     1740

gugauagcua cauagugauc gggguuggcg acaagaaaau uacccaccau uggcaccggu     1800

cuggaucaac ucuaggcaag gcguucagua caacccucaa aggggcucag cgccuggccg     1860

cacuuggaga uacagccugg gacuuuggua gcaucggcgg ggucuucaau uccauaggca     1920

gagcugugca ucaaguguuu ggaggugccu ucaggacuuu guuugggggc augucuugga     1980

uuacccaggg acugaugggc gcacuccugc uuuggauggg ggucaacgcu cgugaucggu     2040

cgaucgcccu ggcguucuug gccacgggag gcguucucgu guuucuggcu accaacgugc     2100

acgcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10460
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10460
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcguu acuaggcggg      120

gcuccgccua cuauauguac cuugaucgca acgacgcggg ggaggcuauu ucuuuuccca      180

cgacccuggg aaugaauaag ugcuauauac agaucaugga uuugggucau augugugacg      240

ccacaauguc auacgaaugc ccuauguuag augagggcgu ggaaccagac gauguagacu      300

guuggugcaa cacuaccagu acaugggugg ucuaugggac cugucaccau aaaaagggag      360

aggcacgacg uagcagaagg gccgugacuc ugccguccca cucuacacgg aaacuacaaa      420

cccgcucgca gacguggcuc gaaucaagag aguacacuaa gcaucugauu aggguugaga      480

auuggaucuu ccggaacccc ggcuuugcuc uugcagccgc ugccauugcg uggcuguugg      540

guagcaguac cucccagaaa gugaucuauc ucgucaugau acugcugauc gcacccgccu      600

acucuauucg cugcaucggg gugagcaauc gagauuucgu agaaggaaug uccggcggca      660

cauggguuga cgugguccuc gagcacgggg gaugugugac ugugauggcu caggauaagc      720

caaccgucga cauugaauua guuacaacca cggugucaaa cauggccgag guaagaaguu      780

acugcuauga ggcaagcauc ucugauaugg cuuccgacuc aagguguccc acucaaggug      840

aagccuaccu ggacaagcag agcgauacac aguaugugug caaacggacc cuugucgacc      900

guggcugggg gaauggaugu ggcuuguuug guaaggggag ucuggugacu ugcgcgaaau      960

ucgccugcuc uaagaaaaug acaggaaagu ccauacagcc ugagaaccua gaauaccgca     1020

uuaugcucag cguucacggc ucgcaacauu cuggcaugau cgugaaugau accgggcacg     1080

agacggacga aaacagagca aaggucgaga uuaccccaaa uucccccagg gcugaggcca     1140

cacugggagg uuuuggcuca cuugggcugg auugugaacc ucggacugga uuggacuuca     1200

gugaucucua uuaccugacc augaacaaua aacauugguu agugcacaag gagugguuuc     1260

acgacauccc acugccgugg caugcuggcg ccgauacagg uacuccccac uggaacaaua     1320

aagaagcacu cguagaguuc aaggacgccc augcuaaacg acagaccguu gugguccuug     1380

ggagccagga aggcgcggug cacacagcac uggccggagc uuuggaggcc gagauggaug     1440

gcgcaaaggg gcgccuaucc ucuggacauc ugaaaugcag acucaagaug gacaagcuga     1500

ggcuuaaagg ugucagcuau ucacugugua ccgccgcuuu uacuuucacg aagaucccug     1560

ccgaaacauu gcacggcacc gugacuguug aggugcagua cgcagggacc gacggaccau     1620

gcaaaguacc cgcgcaaaug gcugucgaua ugcagacacu caccccugug ggccggcuga     1680

uuacugccaa cccaguuaua acagaaagua cggagaacuc caagaugaug uuagaacugg     1740

acccuccguu uggugauagc uacaucguga uuggggucgg cgagaaaaag aucacccacc     1800

auuggcaccg cucuggauca acucuaggca aggcauucag uacaacccuc aaaggggccc     1860

agagacuggc ugcccuugga gacacagcau gggauuucgg uagcauaggc gggguguuua     1920

auuccauugg aagggcugua caucaagugu ucggcggugc cuuucguacu uuguucgggg     1980

gcaugucuug gaucacccag ggacugaugg gcgcgcuccu gcuuuggaug gggguuaacg     2040

cccgggaccg aucgaucgcu cuggcauuuu uggccacggg aggcguccuc guguuccugg     2100

cuaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10461
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10461
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcuguuac caggcggggu ucagccuacu auauguaccu ugaccgcaac gaugcaggcg      180

aggccauaag uuuccccaca acucugggga ugaauaaaug uuauauccag auuauggacu      240

ugggacacau gugcgaugcu acgaugagcu acgaaugucc uauguuagac gagggcgugg      300

aaccagauga cguagauugc ugguguaaca ccacauccac uugggugguc uaugguaccu      360

gccaucacaa gaaaggggag gcacgacguu cuagaagggc cgugacacug ccgucgcauu      420

caacccggaa gcuacaaacu cgcagccaga cauggcucga aaguagagag uacaccaaac      480

accugaucag gguugagaau uggauuuuuc ggaaccccgg auucgcucuu gccgcggcag      540

ccaucgcuug gcuguugggc uccucuacga gccagaaggu gauauaucuc gucaugaucc      600

ugcugauugc cccugcauac uccauccgcu guauuggcgu gucaaaucga gacuuuguag      660

aagggaugag uggagguacu uggguugaua ucgugcucga gcauggcggg ugcgucaccg      720

ugauggcuca ggacaagcca acaguggaua uagaauuagu cacuaccaca guuagcaaca      780

uggccgaggu gagaucuuac uguuaugagg cguccauuuc agacauggcc agcgauagua      840

ggugccccac ccaaggagaa gcauaccugg acaaacaguc ugacacgcag uauguaugua      900

agcggacucu uguggaucgu ggcuggggua augggugcgg auuguucggc aaaggcuccc      960

uggucacaug cgcuaaguuu gccuguagca aaaagaugac cgggaagucg auccagccug     1020

agaaccuaga auaccgcauu augcucucug ugcacggauc ccaacacuca gguaugaucg     1080

uuaaugacac uggccaugag acagaugaaa acagagcuaa aguggagauc accccaaaua     1140

gucccagggc cgaggcaacg cuugggggau ucggcagccu gggucuggac ugcgaaccuc     1200

ggaccggguu ggauuuuucc gaccucuauu accugacaau gaacaauaag cacugguuag     1260

uccauaaaga gugguuccac gauauuccac ugccguggca cgccggcgcu gacacuggaa     1320

caccccauug gaacaauaag gaagcgcucg uggaguuuaa agaugcacac gccaagcgac     1380

agaccguagu ugugcuuggc ucucaggaag gggcugucca uacugcccug gcaggagccu     1440

uggaggcuga gauggacggu gccaaaggcc gccuaagcuc agggcaccug aaguguagac     1500

ucaagaugga uaaacugagg cuuaagggag ugaguuauuc ccugugcacc gcagcguuca     1560

cauuuaccaa aauaccugcu gaaacuuugc auggcacggu cacaguggag guucaguacg     1620

ccgguaccga cgggccaugu aaggugcccg cacaaauggc cguagacaug cagacucuca     1680

ccccugucgg ccggcugauc acagcuaacc cagugauuac cgaaagcacu gagaacucua     1740

aaaugauguu agaacuggau cccccguucg gagacucaua caucguuaua ggcguggggg     1800

agaagaagau uacacaccac uggcaucgca guggaagcac gcuagguaaa gccuuuucca     1860

ccacucucaa gggcgcacag agacuggcug cccuugggga uacagcgugg gacuucggau     1920

cuaucggcgg ugucuucaau ucgaucggga gggccgugca ccaaguauuu ggcggagcuu     1980

uccguaccuu guuuggcggg augagcugga uuacacaggg acugaugggc gcacuccugc     2040

uuuggauggg ugugaacgcc cgggaucgau ccaucgcucu ggccuucuug gcaacugggg     2100

gaguucucgu cuuucuggcg accaaugugc augccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10462
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10462
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcuguuac caggcggggu ucagccuacu auauguaccu ugaccgcaac gaugcaggcg      180

aggccauaag uuuccccaca acucugggga ugaauaaaug uuauauccag auuauggacu      240

ugggacacac gugcgaugcu accaugagcu acgaaugucc uauguuagac gagggcgugg      300

aaccagauga cguagauugc ugguguaaca caacuuccac cugggugguc uaugguacau      360

gccaucacaa gaaaggggag gcacgacguu cuagaagggc cgugacccug ccgucgcauu      420

caacucggaa gcuacaaaca cgcagccaga ccuggcucga aaguagagag uacacgaaac      480

accugaucag gguugagaau uggauuuuuc ggaaccccgg auucgcucuu gccgcggcag      540

ccaucgcuug gcuguugggc uccucuacua gccagaaggu gauauaucuc gucaugaucc      600

ugcugauugc cccugcauac uccauccgcu guauuggcgu gucaaaucga gacuuuguag      660

aagggaugag uggagguacc uggguugaug ugguccucga gcauggcggg ugcgugacag      720

ugauggcuca ggacaagcca acugucgaua ucgaauuagu uaccacaacc gugagcaaca      780

uggccgaggu aagaucuuac uguuaugagg cguccauauc agacauggcc agcgauagua      840

ggugccccac gcaaggagaa gcauaccugg acaaacaguc ugacacucag uaugugugua      900

agcggacacu ugucgaucgu ggcuggggua augggugcgg auuguucggc aaaggcuccc      960

uggugaccug cgcuaaguuu gccuguagca aaaagaugac ugggaagucg auucagccug     1020

agaaccuaga auaccgcauc augcucucug uucacggauc ccaacacuca gguaugauug     1080

ugaaugacac aggccaugag accgaugaaa acagagcuaa agucgagauc acgccaaaua     1140

gucccagggc cgaggcaacc cuugggggau ucggcagccu gggucuggac ugcgaaccuc     1200

ggacaggguu ggauuuuucc gaccucuauu accugacuau gaacaauaag cacugguuag     1260

ugcauaaaga gugguuccac gauaucccac ugccguggca cgccggcgcu gacaccggaa     1320

caccccauug gaacaauaag gaagcgcucg uagaguuuaa agaugcacac gccaagcgac     1380

agacuguugu gguccuuggc ucucaggaag gggcugugca uaccgcccug gcaggagccu     1440

uggaggcuga gauggacggu gccaaaggcc gccuaagcuc agggcaccug aaguguagac     1500

ucaagaugga uaaacugagg cuuaagggag ucaguuauuc ccugugcaca gcagcguuca     1560

ccuuuacuaa aauuccugcu gaaacguugc auggcacagu gaccguugag gugcaguacg     1620

ccgguacuga cgggccaugu aagguacccg cacaaauggc cgucgacaug cagacccuca     1680

caccuguggg ccggcugaua accgcuaacc caguuaucac ugaaagcaca gagaacucua     1740

aaaugauguu agaacuggau cccccguucg gagacucaua cauugugauc ggcgucgggg     1800

agaagaagau aacgcaccac uggcaucgca guggaagcac ccuagguaaa gccuuuucca     1860

cuacacucaa gggcgcacag agacuggcug cccuugggga uaccgcgugg gacuucggau     1920

cuauuggcgg uguguucaau ucgaucggga gggccguaca ccaaguguuu ggcggagcuu     1980

uccguacauu guuuggcggg augagcugga ucacucaggg acugaugggc gcacuccugc     2040

uuuggauggg uguuaacgcc cgggaucgau ccauugcucu ggccuucuug gcaaccgggg     2100

gaguccucgu guuucuggcg acgaaugugc augccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10463
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10463
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcuauaac caggcggggu ucagccuacu auauguaccu ugaccgcagu gaugcaggca      180

aagccaucag cuucgcuaca acucuggggg uuaacaagug ucacgugcag auuauggacu      240

ugggacauau gugcgaugca acgauguccu augagugucc cauguuagac gaaggcguag      300

agccugauga cguggauugc ugguguaaua ccacaucuac uugggucgug uacgguaccu      360

gccaccauaa aaagggggaa gcccgacguu cgagaagggc uguuacacug ccaucacaca      420

gcacccggaa acuacaaacu cgcagucaga cauggcucga guccagagaa uauaccaagc      480

aucugaucaa gguggagaac uggauuuuua ggaauccggg auucgcccuu gucgcggugg      540

caaucgccug gcuguugggc ucuagcacgu cccagaaagu aauauaccuc guuaugaucc      600

ugcugauugc ucccgccuau ucaauccggu guauuggcgu gaguaaccgc gacuuugucg      660

aggggaugag cggagguacu uggguggaug ugguccucga acacggcggg ugcguuaccg      720

ugauggcaca ggacaagccu acaguagaua ucgaguuagu gacuaccaca gucucuaaua      780

uggcugaagu gcgauccuac uguuacgagg ccucaauaag cgacauggcg agugauucua      840

gaugcccaac ccaaggagag gccuaucugg acaaacaguc cgacacgcag uacguuugua      900

agaggacucu uguggaucgg ggcuggggua acgggugcgg auuguucggc aaaggcagcc      960

uggucacaug cgcaaaguuu accuguucga agaaaaugac ugggaagucu auucagcccg     1020

aaaaucuaga guaucguauc augcucuccg ugcacggauc acaacauagu gguaugauug     1080

gcuacgaaac agacgaggau cgcgcuaaag uagaaguuac cccuaacagc ccaagagccg     1140

aggcuacgcu ugggggauuc ggcucccugg gucuggacug cgagcccagg accggguugg     1200

auuuuucuga ccucuauuac cugacaauga auaacaagca cugguuagug cauaaagaau     1260

gguuccacga uaucccucug ccauggcacg ccggcgcaga cacuggaacc ccgcauugga     1320

auaacaagga ggcccucguc gaauuuaaag augcucacgc gaagcggcag acaguggucg     1380

ugcuuggcag ccaggagggg gcaguucaua cugcccuggc uggagccuug gaagcagaga     1440

uggacggugc caagggccga cuauucucag ggcaccugaa augucgccuc aagauggaua     1500

aacugagacu uaagggagug aguuauuccc ugugcaccgc ugccuuuaca uucaccaaag     1560

uacccgcaga gacuuugcau ggcacgguca caguggaagu ucaguacgcg gguaccgacg     1620

ggccuuguaa gaucccagug caaauggcug ucgacaugca gacucucacc cccgugggca     1680

ggcugauuac agccaauccu guaauaaccg agagcacuga aaacucuaag augauguuag     1740

agcuggaucc acccuuugga gacucauaca ucgugauugg cguuggggau aaaaagauca     1800

cacaccacug gcaucggagu ggaagcacgc uagguaaagc auucuccacc acucucaagg     1860

gcgcccagcg ccuggcugcc cuuggggaca cagcauggga uuucggaucu auaggcggug     1920

ucuuuaauuc gauugggaga gcugugcacc aaguguucgg cggagccuuu aggaccuugu     1980

ucggcgggau gagcuggauc acacagggac ugaugggcgc gcuccugcuu uggaugggug     2040

ucaacgcccg ugaccggucc aucgcucugg cauuuuuggc cacuggggga guucucgugu     2100

uccuggcuac caacgugcau gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10464
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10464
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcuguuac caggcggggu ucagccuacu auauguaccu ugaccgcaac gaugcaggcg      180

aggccauaag uuuccccaca acucugggga ugaauaaaug uuauauccag auuauggacu      240

ugggacacau gugcgaugcu acgaugagcu acgaaugucc uauguuagac gagggcgugg      300

aaccagauga cguagauugc ugguguaaca ccacauccac uugggugguc uaugguaccu      360

gccaucacaa gaaaggggag gcacgacguu cuagaagggc cgugacacug ccgucgcauu      420

caacccggaa gcuacaaacu cgcagccaga cauggcucga aaguagagag uacaccaaac      480

accugaucag gguugagaau uggauuuuuc ggaaccccgg auucgcucuu gccgcggcag      540

ccaucgcuug gcuguugggc uccucuacga gccagaaggu gauauaucuc gucaugaucc      600

ugcugauugc cccugcauac uccauccgcu guauuggcgu gucaaaucga gacuuuguag      660

aagggaugag uggagguacu uggguugaug ugguccucga gcauggcggg ugcgugaccg      720

ugauggcuca ggacaagcca acagucgaua ucgaauuagu uacuaccaca gugagcaaca      780

uggccgaggu aagaucuuac uguuaugagg cguccauauc agacauggcc agcgauagua      840

ggugccccac ccaaggagaa gcauaccugg acaaacaguc ugacacgcag uaugugugua      900

agcggacucu ugucgaucgu ggcuggggua augggugcgg auuguucggc aaaggcuccc      960

uggugacaug cgcuaaguuu gccuguagca aaaagaugac cgggaagucg auucagccug     1020

agaaccuaga auaccgcauc augcucucug uucacggauc ccaacacuca gguaugauug     1080

ugaaugacac uggccaugag acagaugaaa acagagcuaa agucgagauc accccaaaua     1140

gucccagggc cgaggcaacg cuugggggau ucggcagccu gggucuggac ugcgaaccuc     1200

ggaccggguu ggauuuuucc gaccucuauu accugacaau gaacaauaag cacugguuag     1260

ugcauaaaga gugguuccac gauaucccac ugccguggca cgccggcgcu gacacuggaa     1320

caccccauug gaacaauaag gaagcgcucg uagaguuuaa agaugcacac gccaagcgac     1380

agaccguugu gguccuuggc ucucaggaag gggcugugca uacugcccug gcaggagccu     1440

uggaggcuga gauggacggu gccaaaggcc gccuaagcuc agggcaccug aaguguagac     1500

ucaagaugga uaaacugagg cuuaagggag ucaguuauuc ccugugcacc gcagcguuca     1560

cauuuaccaa aauuccugcu gaaacuuugc auggcacggu gacaguugag gugcaguacg     1620

ccgguaccga cgggccaugu aagguacccg cacaaauggc cgucgacaug cagacucuca     1680

ccccuguggg ccggcugaua acagcuaacc caguuaucac cgaaagcacu gagaacucua     1740

aaaugauguu agaacuggau cccccguucg gagacucaua cauugugauc ggcgucgggg     1800

agaagaagau aacacaccac uggcaucgca guggaagcac gcuagguaaa gccuuuucca     1860

ccacucucaa gggcgcacag agacuggcug cccuugggga uacagcgugg gacuucggau     1920

cuauuggcgg uguguucaau ucgaucggga gggccguaca ccaaguguuu ggcggagcuu     1980

uccguaccuu guuuggcggg augagcugga ucacacaggg acugaugggc gcacuccugc     2040

uuuggauggg uguuaacgcc cgggaucgau ccauugcucu ggccuucuug gcaacugggg     2100

gaguccucgu guuucuggcg accaaugugc augccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10465
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10465
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau ugucgggcug cuccuuacaa cugccauggc ugcaguuacg cugcccuccc      120

acucuaccag aaaguugcag acaaggucac aaacuugguu agagagucgg gaauacacca      180

aacaucugau ucgcguggag aacuggauau uccgaaaucc uggauuugcc cuagcggcug      240

ccgcaaucgc cuggcuccug gguagcucca caucucagaa gguaauuuau cuugugauga      300

uccuguugau ugcuccagca uacucgaucc guugcauagg cgucucaaac agagacuucg      360

uggaagggau gagcggaggc accuggguug auaucgugcu cgagcacggu ggguguguca      420

cugugauggc ccaggacaaa ccgacaguag auauugaacu gguuaccacg acugugagua      480

auauggcuga ggucaggucc uauugcuacg aggccucuau cagcgacaug gcguccgauu      540

cacggugucc cacccaggga gaagcauauc uggacaagca aagugauaca caguacgugu      600

gcaaacgcac ucucguggac agaggcuggg gcaacgggug uggauuauuu gguaagggca      660

gccuggucac augcgccaag uucgcuuguu cuaaaaagau gacagggaaa uccauucagc      720

cugagaaucu ugaauauagg aucauguugu caguucaugg aagccagcac aguggcauga      780

uagugaacga uaccggucau gagacggacg agaaucgggc caagguagaa auuacuccaa      840

acucuccccg cgcagaggcu acacuagggg gauuuggcuc ccugggccuc gauugcgaac      900

cucgaaccgg gcuggacuuc agcgaucuuu acuaccugac uaugaauaac aaacacuggu      960

uggugcacaa ggagugguuu caugacaucc cacuccccug gcacgccgga gcggacacag     1020

guaccccuca uuggaauaac aaggaagccc uggucgaguu caaagaugca cacgcuaaga     1080

gacaaacggu gguuguguua ggcucgcagg agggggccgu ccacaccgcu cuggccggag     1140

cacucgaagc cgagauggac ggcgcuaaag guaggcuuuc uuccgggcau cugaaguguc     1200

gguugaaaau ggauaagcua cgucugaaag gcgugucaua uagucucugc acagcggcau     1260

uuacuuucac caagauucca gccgaaacac ugcacggaac uguaaccguu gaggugcagu     1320

acgcuggcac agacgggccg uguaaggucc ccgcccagau ggcaguggau augcaaaccc     1380

uuacuccugu cggacgccug aucacggcca auccagugau cacagaaagc accgagaacu     1440

ccaaaaugau guuggagcuc gaucccccuu uuggugacuc uuauauuguu auaggcgugg     1500

gggaaaagaa aaucacucau cacuggcaua gaagcggauc aaccauuggc aaggcuuucg     1560

aggccacagu aaggggugca aaacggaugg cgguccuggg ggacaccgcu ugggauuuug     1620

gcaguguggg aggcgccuua aauucccugg ggaagggaau ccaccagaua uucggugcag     1680

ccuucaagag ccuauuuggc gggaugucuu gguucucaca gauucucauc ggaacucugc     1740

uuaugugguu gggccugaac accaaaaacg guaguaucag ccucaugugc cuggcucuug     1800

ggggcguucu gaucuuuuug uccacggccg ugucugcaug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10466
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10466
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcaguuacg cugcccuccc      120

acucuaccag aaaguugcag acaaggucac aaacuugguu agagagucgg gaauacacca      180

aacaucugau ucgcguggag aacuggauau uccgaaaucc uggauuugcc cuagcggcug      240

ccgcaaucgc cuggcuccug gguagcucca caucucagaa gguaauuuau cuugugauga      300

uccuguugau ugcuccagca uacucgaucc guugcauagg cgucucaaac agagacuucg      360

uggaagggau gagcggaggc accuggguug augugguccu cgagcacggu ggguguguga      420

cuguaauggc ccaggacaaa ccgacaguug auaucgaacu ggugaccacg acugucagua      480

auauggcuga ggugaggucc uauugcuacg aggccucuau uagcgacaug gcguccgauu      540

cacggugucc cacccaggga gaagcauauc uggacaagca aagugauaca caguacgugu      600

gcaaacgcac ucucgucgac agaggcuggg gcaacgggug uggauuauuu gguaagggca      660

gccugguuac cugcgccaag uucgcuuguu cuaaaaagau gacagggaaa uccauccagc      720

cugagaaucu ugaauauagg auuauguugu cagugcaugg aagccagcac aguggcauga      780

ucguaaacga uaccggucau gagacggacg agaaucgggc caagguggaa auaacuccaa      840

acucuccccg cgcagaggcu acacugggag gauuuggcuc ccuaggccuc gauugcgaac      900

cucgaaccgg gcuggacuuc agcgaucuuu acuaccugac uaugaauaac aaacacuggu      960

ugguccacaa ggagugguuu caugacauuc cacuccccug gcacgccgga gcggacacag     1020

guaccccuca uuggaauaac aaggaagccc ugguggaguu caaagaugca cacgcuaaga     1080

gacaaacggu uguggucuua ggcucgcagg agggggccgu gcacaccgcu cuggccggag     1140

cacucgaagc cgagauggac ggcgcuaaag guaggcuuuc uuccgggcau cugaaguguc     1200

gguugaaaau ggauaagcua cgucugaaag gcguaucaua uagucucugc acagcggcau     1260

uuacuuucac caagauccca gccgaaacac ugcacggaac uguuaccgug gagguccagu     1320

acgcuggcac agacgggccg uguaaggugc ccgcccagau ggcagucgau augcaaaccc     1380

uuacuccugu gggacgccug auuacggcca auccaguuau cacagaaagc accgagaacu     1440

ccaaaaugau guuggagcuc gaucccccuu uuggugacuc uuauaucgug auuggcguag     1500

gggaaaagaa aauaacucau cacuggcaua gaagcggauc aaccaucggc aaggcuuucg     1560

aggccacagu caggggugca aaacggaugg cggugcuggg ggacaccgcu ugggauuuug     1620

gcaguguugg aggcgccuua aauucccugg ggaaggggau ucaccagauc uucggugcag     1680

ccuucaagag ccuauuuggc gggaugucuu gguucucaca gauacucauu ggaacucugc     1740

uuaugugguu gggccugaac gcuaaaaacg guaguaucag ccucaugugc cuggcccuug     1800

ggggcgugcu gaucuuuuug uccacagcag ucucugcuug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10467
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10467
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uauagggcug cuccuuacaa cugccauggc ugcagugacg cugcccuccc      120

acucuaccag aaaguugcag acaaggucac aaacuugguu agagagucgg gaauacacca      180

aacaucugau caaggucgag aacuggauuu uccgcaaucc uggauuugcc cuaguugcgg      240

uggcuaucgc cuggcuccug gguagcucca caucucagaa aguaauuuau cuugugauga      300

uccuguugau agcaccagcc uacucgaucc gaugcauugg cgucucaaac cgugacuucg      360

uggaagggau gagcggaggc accuggguug augugguccu cgagcacggu ggguguguga      420

cuguaauggc ucaggacaag ccgacaguug auaucgaacu ggugaccacg acugucagua      480

auauggcaga ggugagaucc uauugcuacg aggccucuau uagcgacaug gcuuccgauu      540

caaggugucc cacccaggga gaagccuauc uggacaaaca aagugauaca caguacgugu      600

gcaagcggac ucucgucgac cgcggcuggg gcaacgggug uggauuauuu gguaagggca      660

gccugguuac cugcgcgaaa uucacauguu cuaagaaaau gaccgggaag uccauccagc      720

cugagaaucu ugaauauaga auaauguugu cagugcaugg aagccagcac aguggcauga      780

uugguuacga gacggaugag gacagggcaa aaguagaagu gacuccaaac ucuccccggg      840

ccgaggcuac acuuggggga uuuggcuccc uaggccucga uugcgaaccu cgcaccgggc      900

uggacuucag cgaucuuuac uaucugacua ugaauaacaa gcauugguug guccacaagg      960

agugguuuca cgacauccca cuccccuggc augccggagc agacacaggu accccucacu     1020

ggaauaacaa agaagcucug guggaguuca aggaugccca ugcgaaacga caaacgguug     1080

uggucuuagg cucgcaggag ggggccgugc acaccgcacu ggcuggagcc cucgaagcug     1140

agauggacgg cgccaagggu agacuguuuu cugggcaccu gaaauguagg uugaagaugg     1200

auaaacuacg gcugaagggc guauccuacu cacucugcac agcagccuuc acuuuuacca     1260

agguuccagc ugaaacacug cauggaacug ugaccgucga ggugcaguau gcgggcacag     1320

acgggccgug uaaaauuccc guccagaugg caguggauau gcaaacccuu acuccuguug     1380

gacgucugau cacggccaau ccagugauca cagaaaguac cgagaacagc aagaugaugu     1440

uggagcucga ucccccuuuc ggugacuccu acauuguaau aggcgucggg gacaaaaaga     1500

ucacucacca uuggcaccgc ucuggaagca ccauuggcaa ggcuuuugaa gccacaguga     1560

gaggugcaaa aaggauggcc guucuggggg auaccgcuug ggacuucggc ucagugggag     1620

gcgucuucaa uaguuuaggg aagggaaucc aucagauauu uggugccgca uucaaauccc     1680

uguuuggcgg gaugagcugg uucucucaga uucuaaucgg aacucuccug guguggcuug     1740

gcuugaacac aaagaauggu ucaaucaguc ugacgugccu cgcgcuuggg ggcguaauga     1800

uuuuucugag caccgcugug uccgccugag gacuagugca ucacauuuaa aagcaucuca     1860

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     1920

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     1980

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2100

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2158


<210>  10468
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10468
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccuuacaa cugccauggc ugcaguuacg cugcccuccc      120

acucuaccag aaaguugcag acaaggucac aaacuugguu agagagucgg gaauacacca      180

aacaucugau ucgcguggag aacuggauau uccgaaaucc uggauuugcc cuagcggcug      240

ccgcaaucgc cuggcuccug gguagcucca caucucagaa gguaauuuau cuugugauga      300

uccuguugau ugcuccagca uacucgaucc guugcauagg cgucucaaac agagacuucg      360

uggaagggau gagcggaggc accuggguug augugguccu cgagcacggu ggguguguga      420

cuguaauggc ccaggacaaa ccgacaguug auaucgaacu ggugaccacg acugucagua      480

auauggcuga ggugaggucc uauugcuacg aggccucuau uagcgacaug gcguccgauu      540

cacggugucc cacccaggga gaagcauauc uggacaagca aagugauaca caguacgugu      600

gcaaacgcac ucucgucgac agaggcuggg gcaacgggug uggauuauuu gguaagggca      660

gccugguuac cugcgccaag uucgcuuguu cuaaaaagau gacagggaaa uccauccagc      720

cugagaaucu ugaauauagg auuauguugu cagugcaugg aagccagcac aguggcauga      780

ucguaaacga uaccggucau gagacggacg agaaucgggc caagguggaa auaacuccaa      840

acucuccccg cgcagaggcu acacugggag gauuuggcuc ccuaggccuc gauugcgaac      900

cucgaaccgg gcuggacuuc agcgaucuuu acuaccugac uaugaauaac aaacacuggu      960

ugguccacaa ggagugguuu caugacauuc cacuccccug gcacgccgga gcggacacag     1020

guaccccuca uuggaauaac aaggaagccc ugguggaguu caaagaugca cacgcuaaga     1080

gacaaacggu uguggucuua ggcucgcagg agggggccgu gcacaccgcu cuggccggag     1140

cacucgaagc cgagauggac ggcgcuaaag guaggcuuuc uuccgggcau cugaaguguc     1200

gguugaaaau ggauaagcua cgucugaaag gcguaucaua uagucucugc acagcggcau     1260

uuacuuucac caagauccca gccgaaacac ugcacggaac uguuaccgug gagguccagu     1320

acgcuggcac agacgggccg uguaaggugc ccgcccagau ggcagucgau augcaaaccc     1380

uuacuccugu gggacgccug auuacggcca auccaguuau cacagaaagc accgagaacu     1440

ccaaaaugau guuggagcuc gaucccccuu uuggugacuc uuauaucgug auuggcguag     1500

gggaaaagaa aauaacucau cacuggcaua gaagcggauc aaccaucggc aaggcuuucg     1560

aggccacagu caggggugca aaacggaugg cggugcuggg ggacaccgcu ugggauuuug     1620

gcaguguugg aggcgccuua aauucccugg ggaaggggau ucaccagauc uucggugcag     1680

ccuucaagag ccuauuuggc gggaugucuu gguucucaca gauacucauu ggaacucugc     1740

uuaugugguu gggccugaac acgaaaaacg guaguaucag ccucaugugc cuggcucuug     1800

ggggcgugcu gaucuuuuug uccacagccg ucucugcaug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10469
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10469
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgc ggugacccuu ccuucacaca guacaagaaa gcugcaaacu aggagccaga      180

cgugguugga aucccgggag uacaccaaac auuuaauccg cguagaaaac uggauuuucc      240

gaaauccagg auuugcucug gccgcagccg cuaucgcaug gcuacucggu ucuucgacau      300

cacagaaggu gauuuaucug gucaugaucc uucugauagc cccggcuuac agcauccguu      360

gcauuggcgu gaguaacaga gacuucguug aggggauguc cggaggcacu uggguggaua      420

ucgucuugga acacgguggg ugugugaccg uaauggccca ggacaaaccc acaguugaua      480

uugagcucgu gaccacuaca gucucuaaua uggcggaggu gaggagcuau ugcuacgaag      540

cauccaucuc agacauggcc agugauagcc gguguccuac ccaaggagag gcuuaucugg      600

acaagcaguc ugauacgcag uacgugugca aacgcacucu ggucgacaga ggcuggggca      660

acgggugugg acucuuuggu aagggcuccu uaguuaccug cgccaaguuc gcauguagca      720

aaaagaugac agggaaauca auacagccag aaaaucugga guauaggauu augcuuagug      780

ugcauggauc ucaacacucc ggcaugaucg uaaacgauac uggucaugag accgacgaaa      840

aucgggcuaa gguggagauu acacccaaca gcccucgcgc cgaagcgacc uugggaggau      900

uuggcucgcu gggccuagau ugcgagccac gaacggggcu cgacuucucu gaucuguacu      960

accuuacuau gaauaacaaa cacuggcugg uccacaagga augguuucau gacauccccu     1020

ugccuuggca cgccggagca gacacaggua ccccacauug gaauaacaag gaggcucucg     1080

uggaguucaa agaugcccac gcuaagagac agacuguugu gguccugggc ucccaggaag     1140

gggccgugca cacagcauua gccggagcuc uggaggcgga aauggacggc gcaaaaggua     1200

ggcucucaag ugggcaucuu aagugucggc ugaaaaugga uaaguugcgu cuaaaaggcg     1260

uaagcuauuc ccugugcacc gccgcuuuua cguucaccaa gaucccggcc gagacacucc     1320

acggaacugu uaccguggaa guccaguacg caggcacaga cgggcccugu aaggugccug     1380

cccaaauggc ugucgauaug cagacucuga ccccaguggg acgccuuauu acagccaauc     1440

ccguuauaac cgagucuacu gagaacagca aaaugaugcu ggaauuggac ccuccauuug     1500

gugauucaua uaucgugauu ggcguagggg agaagaaaau cacgcaucac uggcauagaa     1560

guggauccac aauaggcaag gcauucgaag cgaccgucag gggugcuaaa cggauggccg     1620

ugcucgggga cacugcaugg gauuucggca gcguuggagg cgccuuaaau ucucugggga     1680

aggggauuca ccagaucuuu ggugcugccu ucaagucacu guuuggcggg augaguuggu     1740

ucagccaaau ccuaauugga acccuccuga uguggcuugg cuugaacacc aaaaacgguu     1800

ccaucucucu gaugugccuc gcacuugggg gcgugcugau uuuucugucg acagcuguca     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10470
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10470
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgc ggugacccuu ccuucacaca guacaagaaa gcugcaaacu aggagccaga      180

cgugguugga aucccgggag uacaccaaac auuuaauccg cguagaaaac uggauuuucc      240

gaaauccagg auuugcucug gccgcagccg cuaucgcaug gcuacucggu ucuucgacau      300

cacagaaggu gauuuaucug gucaugaucc uucugauagc cccggcuuac agcauccguu      360

gcauuggcgu gaguaacaga gacuucguug aggggauguc cggaggcacu uggguggaug      420

ucguguugga acacgguggg uguguaaccg uuauggccca ggacaaaccc acaguggaua      480

ucgagcucgu caccacuaca gugucuaaua uggcggaggu gaggagcuau ugcuacgaag      540

cauccauuuc agacauggcc agugauagcc gguguccuac ccaaggagag gcuuaucugg      600

acaagcaguc ugauacgcag uacgucugca aacgcacucu gguugacaga ggcuggggca      660

acgggugugg acucuuuggu aagggcuccu uagugaccug cgccaaguuc gcauguagca      720

aaaagaugac agggaaauca auccagccag aaaaucugga guauaggaua augcuuagug      780

uacauggauc ucaacacucc ggcaugauug ugaacgauac uggucaugag accgacgaaa      840

aucgggcuaa ggucgagauc acacccaaca gcccucgcgc cgaagcgacc uugggagggu      900

uuggcucgcu gggccuagau ugcgagccac gaacggggcu cgacuucucu gaucuguacu      960

accuuacuau gaauaacaaa cacuggcugg ugcacaagga augguuucau gacauucccu     1020

ugccuuggca cgccggagca gacacaggua ccccacauug gaauaacaag gaggcucucg     1080

uugaguucaa agaugcccac gcuaagagac agacuguggu cgugcugggc ucccaggaag     1140

gggccguaca cacagcauua gccggagcuc uggaggcgga aauggacggc gcaaaaggua     1200

ggcucucaag ugggcaucuu aagugucggc ugaaaaugga uaaguugcgu cuaaaaggcg     1260

uuagcuauuc ccugugcacc gccgcuuuua cguucaccaa gaucccggcc gagacacucc     1320

acggaacugu gaccgucgaa gugcaguacg caggcacaga cgggcccugu aaggucccug     1380

cccaaauggc uguggauaug cagacucuga ccccaguugg acgccuuauc acagccaauc     1440

ccgugauuac cgagucuacu gagaacagca aaaugaugcu ggaauuggac ccuccauuug     1500

gugauucaua uauaguaauc ggcgucgggg agaagaaaau uacgcaucac uggcauagaa     1560

guggauccac aaucggcaag gcauucgaag cgaccgugag gggugcuaaa cggauggccg     1620

uucucgggga cacugcaugg gauuucggca gcgugggagg cgccuuaaau ucucugggga     1680

agggaauaca ccagauuuuu ggugcugccu ucaagucacu guuuggcggg augaguuggu     1740

ucagccaaau ccuaaucgga acccuccuga uguggcuugg cuugaacgcu aaaaacgguu     1800

ccauuucucu gaugugccuc gcacuugggg gcguccugau cuuucugucg acagccguga     1860

gcgcgugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10471
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10471
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgc ggugacccuu ccuucacaca guacaagaaa gcugcaaacu aggagccaga      180

cgugguugga aucccgggag uacaccaaac auuuaaucaa gguagaaaac uggauuuucc      240

gcaauccagg auuugcucug guggccgucg caaucgccug gcuacucggu ucuucgacau      300

cacagaaagu gauuuaucug guuaugaucc uucugauagc uccggcauac agcauccgau      360

gcauuggcgu gaguaaccgu gacuucgucg aggggauguc cggaggcacu uggguggaug      420

uaguuuugga acacgguggg ugugugaccg ucauggccca ggacaagccc acaguggaua      480

ucgagcucgu gaccacuaca gucucuaaua uggcugaggu uagaagcuau ugcuacgaag      540

ccuccauuuc agacauggcg agugauucua gguguccuac ccaaggagag gcauaucugg      600

acaaacagag cgauacgcag uacgugugca agcggacucu gguagaccgc ggcuggggca      660

acgggugugg acucuuuggu aagggcuccu uagugaccug cgccaaauuc acauguucaa      720

agaaaaugac uggaaagagc auccagccag aaaaucugga guauagaaua augcuuagug      780

uccauggguc ucaacacucc ggcaugauug guuacgagac cgaugaagac agggcuaaag      840

uggagguuac acccaacagc ccucgggccg aagcaaccuu gggaggguuu ggcucgcugg      900

gccuagauug cgagccacgc acggggcucg acuucucuga ucuguacuau cuuacuauga      960

auaacaagca uuggcuggug cacaaggaau gguuucacga cauccccuug ccuuggcaug     1020

cuggagccga cacagguacc ccacacugga auaacaaaga ggcgcucguc gaguucaagg     1080

augcccaugc aaaacgacag acugugguag uucugggcuc ccaggaaggg gcugugcaca     1140

cagccuuagc uggagcccug gaggcagaaa uggacggcgc caaggguaga cucuuuucag     1200

ggcaccuuaa auguaggcug aagauggaua aauugcggcu aaagggcguc aguuacagcc     1260

ugugcaccgc ugcguucacg uuuaccaagg ugccggcaga gacacuccau ggaacuguca     1320

ccguggaagu ucaguaugcc ggcacagacg ggcccuguaa aauuccugug caaauggcug     1380

uagauaugca gacucugacc ccagucggac gucuuaucac agccaauccc gugaucaccg     1440

aguccacuga gaacucuaag augaugcugg aauuggaccc uccauucggu gauagcuaca     1500

uuguuauagg cgugggggac aaaaagauca cgcaccauug gcaccgcuca ggaaguacaa     1560

uuggcaaagc auucgaggcc accgucagag gugcuaagag gauggccgug cucggggaua     1620

cugcauggga cuuuggcucc guaggaggcg uguucaauag ccuggggaag ggaauccauc     1680

agauauuugg ugcggcuuuc aaaucuuuau uuggcgggau gucaugguuc agucaaauuc     1740

ugaucggaac ccuacucguu uggcugggcc uuaacacaaa gaaugguagc aucuccuuga     1800

ccugccuggc ccucgggggc gucaugauuu uucugucuac ugcagugucg gccugaggac     1860

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     1920

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     1980

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2040

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2160

ggcucuuuuc agagccacca gaauu                                           2185


<210>  10472
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10472
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuauagcugu ucucaugucc gcacaggagu      120

cuugggccgc ggugacccuu ccuucacaca guacaagaaa gcugcaaacu aggagccaga      180

cgugguugga aucccgggag uacaccaaac auuuaauccg cguagaaaac uggauuuucc      240

gaaauccagg auuugcucug gccgcagccg cuaucgcaug gcuacucggu ucuucgacau      300

cacagaaggu gauuuaucug gucaugaucc uucugauagc cccggcuuac agcauccguu      360

gcauuggcgu gaguaacaga gacuucguug aggggauguc cggaggcacu uggguggaug      420

ucguguugga acacgguggg uguguaaccg uuauggccca ggacaaaccc acaguggaua      480

ucgagcucgu caccacuaca gugucuaaua uggcggaggu gaggagcuau ugcuacgaag      540

cauccauuuc agacauggcc agugauagcc gguguccuac ccaaggagag gcuuaucugg      600

acaagcaguc ugauacgcag uacgucugca aacgcacucu gguugacaga ggcuggggca      660

acgggugugg acucuuuggu aagggcuccu uagugaccug cgccaaguuc gcauguagca      720

aaaagaugac agggaaauca auccagccag aaaaucugga guauaggaua augcuuagug      780

uacauggauc ucaacacucc ggcaugauug ugaacgauac uggucaugag accgacgaaa      840

aucgggcuaa ggucgagauc acacccaaca gcccucgcgc cgaagcgacc uugggagggu      900

uuggcucgcu gggccuagau ugcgagccac gaacggggcu cgacuucucu gaucuguacu      960

accuuacuau gaauaacaaa cacuggcugg ugcacaagga augguuucau gacauucccu     1020

ugccuuggca cgccggagca gacacaggua ccccacauug gaauaacaag gaggcucucg     1080

uugaguucaa agaugcccac gcuaagagac agacuguggu cgugcugggc ucccaggaag     1140

gggccguaca cacagcauua gccggagcuc uggaggcgga aauggacggc gcaaaaggua     1200

ggcucucaag ugggcaucuu aagugucggc ugaaaaugga uaaguugcgu cuaaaaggcg     1260

uuagcuauuc ccugugcacc gccgcuuuua cguucaccaa gaucccggcc gagacacucc     1320

acggaacugu gaccgucgaa gugcaguacg caggcacaga cgggcccugu aaggucccug     1380

cccaaauggc uguggauaug cagacucuga ccccaguugg acgccuuauc acagccaauc     1440

ccgugauuac cgagucuacu gagaacagca aaaugaugcu ggaauuggac ccuccauuug     1500

gugauucaua uauaguaauc ggcgucgggg agaagaaaau uacgcaucac uggcauagaa     1560

guggauccac aaucggcaag gcauucgaag cgaccgugag gggugcuaaa cggauggccg     1620

uucucgggga cacugcaugg gauuucggca gcgugggagg cgccuuaaau ucucugggga     1680

agggaauaca ccagauuuuu ggugcugccu ucaagucacu guuuggcggg augaguuggu     1740

ucagccaaau ccuaaucgga acccuccuga uguggcuugg cuugaacacc aaaaacgguu     1800

ccauuucucu gaugugccuc gcacuugggg gcguccugau cuuucugucg acagcuguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10473
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10473
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcgcc guuacucuuc      120

ccucccauuc uacgaggaag cugcagaccc ggucacaaac augguuggag agucgcgaau      180

acacuaaaca cuuaauucga guggagaacu ggauauuucg uaauccuggc uucgcgcugg      240

cugccgcagc caucgcuugg cuacucggga gcuccaccuc ucagaaggua auuuaucugg      300

ugaugauccu ucugauugca ccagccuacu cgaucagaug cauaggaguc ucaaacaggg      360

auuuugugga agguaugagc ggcgggacau ggguugacau cguguuggag cauggaggcu      420

gugucaccgu gauggcucag gauaaaccga cuguagacau ugaacucguu acaaccacgg      480

ugaguaauau ggccgagguc cgguccuauu gcuacgaggc gucuaucagc gauauggcau      540

ccgacucacg cugucccacu cagggugaag ccuaucugga uaagcaaagu gacacccagu      600

acgugugcaa aagaacacug guggauaggg gguggggaaa cggcuguggc cucuucggga      660

aggggagcuu agucacuugc gcuaaguuug ccuguucuaa aaagaugacc gguaaaucca      720

uucagccuga gaaucuggaa uaucggauca ugcuuucagu ucacggcagc cagcauagug      780

ggaugauagu gaacgacaca ggacacgaga ccgaugagaa ucgcgcaaag guagaaauua      840

cgccaaacuc uccccgagcu gaggccacuu ugggcgguuu cgggucccug ggacuagacu      900

gcgaaccuag aacaggccuc gauuuuagcg accuguacua ccuuaccaug aauaacaaac      960

acuggcuggu gcauaaggag ugguuccacg acaucccauu gcccuggcau gcgggcgccg     1020

auacugggac accucacugg aauaacaagg aagcacucgu cgaguuuaaa gacgcucacg     1080

ccaagaggca aaccgugguu gugcugggau cgcaggaggg ugcuguccau acggccuuag     1140

caggcgcccu ggaagcugag auggaugggg cgaaaggacg gcucucuucc ggccaccuua     1200

agugucgucu gaaaauggac aaguugcgcc uaaaaggugu gucauauagu cugugcaccg     1260

cagccuucac auuuacuaag auuccagcug aaacccucca ugggacagua acuguugagg     1320

ugcaguacgc cggcaccgau ggaccgugua agguccccgc acagauggcc guggacaugc     1380

aaacacugac cccugucggc agacuuauca cugcuaaucc agugaucacg gaaagcacag     1440

agaacuccaa aaugaugcug gaguuggauc ccccuuucgg ggacucuuau auuguuauag     1500

gaguggguga aaagaaaauc acccaccauu ggcacaggag cggcucaacu auugggaagg     1560

ccuuugaggc aaccguacgg ggagcgaaac gaauggcugu ccucggcgau acagccuggg     1620

acuucgguag ugugggaggc gcauuaaauu cccugggaaa gggcauccac cagauauucg     1680

gggccgcuuu uaagagccug uucggaggua ugucuugguu uucacagauu cuaaucggca     1740

cccuccugau guggcuuggg uugaacacua aaaacggaag uaucagccug augugccucg     1800

cccugggcgg uguucuuauu uuccugucca cagcaguguc ugcuugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10474
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10474
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcgcc guuacucuuc      120

ccucccauuc uacgaggaag cugcagaccc ggucacaaac augguuggag agucgcgaau      180

acacuaaaca cuuaauucga guggagaacu ggauauuucg uaauccuggc uucgcgcugg      240

cugccgcagc caucgcuugg cuacucggga gcuccaccuc ucagaaggua auuuaucugg      300

ugaugauccu ucugauugca ccagccuacu cgaucagaug cauaggaguc ucaaacaggg      360

auuuugugga agguaugagc ggcgggacau ggguugacgu ggucuuggag cauggaggcu      420

gugugaccgu aauggcucag gauaaaccga cuguugacau cgaacucgug acaaccacgg      480

ucaguaauau ggccgaggug cgguccuauu gcuacgaggc gucuauuagc gauauggcau      540

ccgacucacg cugucccacu cagggugaag ccuaucugga uaagcaaagu gacacccagu      600

acgugugcaa aagaacacug gucgauaggg gguggggaaa cggcuguggc cucuucggga      660

agggaagccu gguuacuugc gcuaaguuug ccuguucuaa aaagaugacc gguaaaucca      720

uccagccuga gaaucuggaa uaucggauua ugcuuucagu gcacggcagc cagcauagug      780

ggaugaucgu aaacgacaca ggacacgaga ccgaugagaa ucgcgcaaag guggaaauaa      840

cgccaaacuc uccccgagcu gaggccacuu ugggcgguuu cgggucccug ggacuagacu      900

gcgaaccuag aacaggccuc gauuuuagcg accuguacua ccuuaccaug aauaacaaac      960

acuggcuggu ccauaaggag ugguuccacg acauuccauu gcccuggcau gcgggcgccg     1020

auacugggac accucacugg aauaacaagg aagcacucgu ggaguuuaaa gacgcucacg     1080

ccaagaggca aaccguugug guccugggau cgcaggaggg ugcugugcau acggccuuag     1140

caggcgcccu ggaagcugag auggaugggg cgaaaggacg gcucucuucc ggccaccuua     1200

agugucgucu gaaaauggac aaguugcgcc uaaaaggugu aucauauagu cugugcaccg     1260

cagccuucac auuuacuaag aucccagcug aaacccucca ugggacaguu acuguggagg     1320

uccaguacgc cggcaccgau ggaccgugua aggugcccgc acagauggcc gucgacaugc     1380

aaacacugac cccugugggc agacuuauua cugcuaaucc aguuaucacg gaaagcacag     1440

agaacuccaa aaugaugcug gaguuggauc ccccuuucgg ggacucuuau aucgugauug     1500

gaguagguga aaagaaaaua acccaccauu ggcacaggag cggcucaacu aucgggaagg     1560

ccuuugaggc aaccguccgg ggagcgaaac gaauggcugu gcucggcgau acagccuggg     1620

acuucgguag uguugggggc gcauuaaauu ccuuaggaaa gggcauucac cagaucuucg     1680

gggccgcuuu uaagagccug uucggaggua ugucuugguu uucacagaua cuaauuggca     1740

cccuccugau guggcuuggg uugaacgcca aaaacggaag uaucagccug augugccucg     1800

cacugggcgg ugugcuuauc uuccugucca cugcugucuc ugccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10475
<211>  2164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10475
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcgcc guuacucuuc      120

ccucccauuc uacgaggaag cugcagaccc ggucacaaac augguuggag agucgcgaau      180

acacuaaaca cuuaauuaag guggagaacu ggauauuucg aaauccuggc uucgcgcugg      240

uagcuguggc caucgcaugg cuacucggga gcuccaccuc ucagaaaguc auuuaucugg      300

ugaugauccu ucugauugcc ccagcuuacu cgauccguug cauaggaguu ucaaacagag      360

auuuugugga agguaugagc ggcgggacau gggucgacgu gguauuggag cauggaggcu      420

guguuaccgu gauggcacag gauaagccga cugucgacau cgaacucgug acaaccacgg      480

ugaguaauau ggccgagguc agguccuauu gcuacgaggc uucuauuagc gauauggccu      540

ccgacucacg gugucccacu cagggugaag cguaucugga uaaacaaagu gacacccagu      600

acguuugcaa gcgcacacug guggauagag gguggggaaa cggcuguggc cucuucggga      660

aggggagcuu aguaacuugc gcaaaauuua ccuguucuaa gaaaaugaca gguaagucca      720

uccagccuga gaaucuggaa uauaggauua ugcuuucagu gcacggcagc cagcauagug      780

ggaugaucgg auacgagacc gacgaggauc gggccaaagu cgaagugacg ccaaacucuc      840

cccgcgcuga ggccacuuug ggcgguuucg ggucccuggg acuagacugc gaaccucgaa      900

caggccucga uuuuagcgac cuguacuauc uuaccaugaa uaacaagcac uggcugguuc      960

acaaggagug guuccaugac auaccauugc ccuggcacgc aggcgcugau acugggacac     1020

cucauuggaa uaacaaagaa gcccucgugg aguuuaagga cgcgcacgcc aaaagacaaa     1080

ccgucguggu acugggaucg caggagggug caguucacac ggcuuuagcc ggcgcucugg     1140

aagccgagau ggauggggca aagggaaggc uuuucucugg ccaucucaaa ugucggcuga     1200

agauggacaa auugcgucua aagggugugu ccuacucacu gugcaccgcc gcuuuuacau     1260

ucacuaaggu cccagcggaa acccuccacg ggacagugac ugucgaggug caguaugcag     1320

gcaccgaugg accguguaaa auucccguuc agauggccgu ggacaugcaa acacugaccc     1380

cuguaggccg ccuuaucacu gcuaauccag ucauuacgga aaguacagag aacagcaaga     1440

ugaugcugga guuggauccc ccuuuugggg acuccuacau cgugaucgga guuggugaua     1500

aaaagauuac ccaucacugg cauagaucug gcagcacuau agggaaagcc uucgaagcaa     1560

ccgugagggg agccaagcgg auggcugucc ucggcgacac agccugggau uucgguucag     1620

ugggaggcgu auuuaauagu cugggaaagg gcauccacca gauuuucggg gcagcguuua     1680

aauccuuauu cggagguaug agcugguuuu cucagauccu gauaggcacc cuacucgugu     1740

ggcuggggcu uaacacuaag aauggaucaa uuaguuugac augccuggcu cucggcggug     1800

uuaugaucuu ccugagcacg gccgucuccg caugaggacu agugcaucac auuuaaaagc     1860

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     1920

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     1980

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2160

aauu                                                                  2164


<210>  10476
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10476
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug caacaagagu ccacagcgcc guuacucuuc      120

ccucccauuc uacgaggaag cugcagaccc ggucacaaac augguuggag agucgcgaau      180

acacuaaaca cuuaauucga guggagaacu ggauauuucg uaauccuggc uucgcgcugg      240

cugccgcagc caucgcuugg cuacucggga gcuccaccuc ucagaaggua auuuaucugg      300

ugaugauccu ucugauugca ccagccuacu cgaucagaug cauaggaguc ucaaacaggg      360

auuuugugga agguaugagc ggcgggacau ggguugacgu ggucuuggag cauggaggcu      420

gugugaccgu aauggcucag gauaaaccga cuguugacau cgaacucgug acaaccacgg      480

ucaguaauau ggccgaggug cgguccuauu gcuacgaggc gucuauuagc gauauggcau      540

ccgacucacg cugucccacu cagggugaag ccuaucugga uaagcaaagu gacacccagu      600

acgugugcaa aagaacacug gucgauaggg gguggggaaa cggcuguggc cucuucggga      660

agggaagccu gguuacuugc gcuaaguuug ccuguucuaa aaagaugacc gguaaaucca      720

uccagccuga gaaucuggaa uaucggauua ugcuuucagu gcacggcagc cagcauagug      780

ggaugaucgu aaacgacaca ggacacgaga ccgaugagaa ucgcgcaaag guggaaauaa      840

cgccaaacuc uccccgagcu gaggccacuu ugggcgguuu cgggucccug ggacuagacu      900

gcgaaccuag aacaggccuc gauuuuagcg accuguacua ccuuaccaug aauaacaaac      960

acuggcuggu ccauaaggag ugguuccacg acauuccauu gcccuggcau gcgggcgccg     1020

auacugggac accucacugg aauaacaagg aagcacucgu ggaguuuaaa gacgcucacg     1080

ccaagaggca aaccguugug guccugggau cgcaggaggg ugcugugcau acggccuuag     1140

caggcgcccu ggaagcugag auggaugggg cgaaaggacg gcucucuucc ggccaccuua     1200

agugucgucu gaaaauggac aaguugcgcc uaaaaggugu aucauauagu cugugcaccg     1260

cagccuucac auuuacuaag aucccagcug aaacccucca ugggacaguu acuguggagg     1320

uccaguacgc cggcaccgau ggaccgugua aggugcccgc acagauggcc gucgacaugc     1380

aaacacugac cccugugggc agacuuauua cugcuaaucc aguuaucacg gaaagcacag     1440

agaacuccaa aaugaugcug gaguuggauc ccccuuucgg ggacucuuau aucgugauug     1500

gaguagguga aaagaaaaua acccaccauu ggcacaggag cggcucaacu aucgggaagg     1560

ccuuugaggc aaccguccgg ggagcgaaac gaauggcugu gcucggcgau acagccuggg     1620

acuucgguag uguugggggc gcauuaaauu ccuuaggaaa gggcauucac cagaucuucg     1680

gggccgcuuu uaagagccug uucggaggua ugucuugguu uucacagaua cuaauuggca     1740

cccuccugau guggcuuggg uugaacacua aaaacggaag uaucagccug augugccucg     1800

cccugggcgg ugugcuuauc uuccugucca cagcagucuc ugcuugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10477
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10477
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcugccgu uacccuuccc ucacacagua caaggaaacu gcagacucgg agccaaacgu      180

gguuggaguc ccgcgaauac accaagcauu uaauacgagu ggagaacugg aucuuccgua      240

auccugguuu ugcacuggcc gcugcagcca uugcuuggcu acucgggucu ucgacaucac      300

agaaaguaau cuaucuggug augauucuuc ugaucgcccc agcguacagc auaagaugua      360

ucggggucag uaacagggac uucguggaag gaauguccgg cgguacuugg guugauauug      420

uguuggagca cgggggaugc gucaccguga uggcacagga caagccgaca guagauaucg      480

aacucguuac cacuacagug ucuaauaugg ccgagguccg gagcuauugu uacgaggcuu      540

ccauuucaga cauggccagu gauagccgcu gccccaccca gggcgaagca uaucuggaca      600

aacaaucuga uacgcaguac guguguaaga gaacucuggu ggacaggggc ugggggaacg      660

gaugcggucu cuuuggcaag ggguccuuag ucaccugugc uaaauucgcc ugcucaaaga      720

aaaugacagg aaagagcauc cagccugaga aucuggaaua ucggauaaug cuuaguguuc      780

auggcucuca gcacuccggu augauuguga acgauacugg gcaugagacc gacgagaauc      840

gcgcgaaagu agaaaucaca ccaaacagcc cccgagccga ggcaaccuug ggaggcuuug      900

gcucgcuggg gcuagauugu gaaccuagaa cgggacucga cuucucugau cuguacuacc      960

uuacuaugaa uaacaagcac uggcuggugc acaaggagug guuucaugac auuccauugc     1020

ccuggcacgc uggugccgac acaggcaccc cucauuggaa uaacaaagaa gcucucgucg     1080

aguucaagga ugcccacgca aaaaggcaaa cugugguugu gcuggggucc caggagggag     1140

ccguccacac agcuuuagcg ggcgcacugg aagccgagau ggacggugcu aaggggcggc     1200

ucucaagugg ccaucuuaaa ugccgucuga agauggauaa auugcgccua aagggaguga     1260

gcuauucccu guguaccgcc gcauuuacgu ucaccaagau cccagccgaa acacuccacg     1320

gcacuguaac cguugaggug caguacgcug ggacagacgg accgugcaaa guccccgccc     1380

agauggcagu ggauaugcaa acucugaccc cugucgguag acuuaucaca gcgaauccag     1440

ugauuaccga aucuacugag aacagcaaga ugaugcugga guuggauccc ccuuuuggcg     1500

acucauauau aguuaucggg gugggagaga aaaagauuac gcaucacugg cauaggagug     1560

gcuccacaau cgguaaggcu uucgaagcca ccguacgggg cgcaaaacga auggccgucc     1620

ucggcgacac ugcuugggau uuuggaagcg ugggcggggc cuuaaauucu cugggaaagg     1680

guauacacca gauuuucggc gcagcuuuca aaucacuguu ugggggaaug aguugguuca     1740

gccagauccu aaucggcacc cuccugaugu ggcuugguuu gaacacaaag aacgggucca     1800

uuucucugau gugccucgcc cugggcggag uucuuaucuu ucugucgacc gcggugagcg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10478
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10478
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcugccgu uacccuuccc ucacacagua caaggaaacu gcagacucgg agccaaacgu      180

gguuggaguc ccgcgaauac accaagcauu uaauacgagu ggagaacugg aucuuccgua      240

auccugguuu ugcacuggcc gcugcagcca uugcuuggcu acucgggucu ucgacaucac      300

agaaaguaau cuaucuggug augauucuuc ugaucgcccc agcguacagc auaagaugua      360

ucggggucag uaacagggac uucguggaag gaauguccgg cgguacuugg guugaugugg      420

ucuuggagca cgggggaugc gugaccguaa uggcacagga caagccgaca guugauauug      480

aacucgugac cacuacaguc ucuaauaugg ccgaggugcg gagcuauugu uacgaggcuu      540

ccaucucaga cauggccagu gauagccgcu gccccaccca gggcgaagca uaucuggaca      600

aacaaucuga uacgcaguac guguguaaga gaacucuggu cgacaggggc ugggggaacg      660

gaugcggucu cuuuggcaag ggguccuuag uuaccugugc uaaauucgcc ugcucaaaga      720

aaaugacagg aaagagcauu cagccugaga aucuggaaua ucggaucaug cuuagugugc      780

auggcucuca gcacuccggu augauaguaa acgauacugg gcaugagacc gacgagaauc      840

gcgcgaaagu ggaaauuaca ccaaacagcc cccgagccga ggcaaccuug ggaggcuuug      900

gcucgcuggg gcuagauugu gaaccuagaa cgggacucga cuucucugau cuguacuacc      960

uuacuaugaa uaacaagcac uggcuggucc acaaggagug guuucaugac aucccauugc     1020

ccuggcacgc uggugccgac acaggcaccc cucauuggaa uaacaaagaa gcucucgugg     1080

aguucaagga ugcccacgca aaaaggcaaa cuguuguggu ccuggggucc caggagggag     1140

ccgugcacac agcuuuagcg ggcgcacugg aagccgagau ggacggugcu aaggggcggc     1200

ucucaagugg ccaucuuaaa ugccgucuga agauggauaa auugcgccua aagggaguaa     1260

gcuauucccu guguaccgcc gcauuuacgu ucaccaagau uccagccgaa acacuccacg     1320

gcacuguuac cguggagguc caguacgcug ggacagacgg accgugcaaa gugcccgccc     1380

agauggcagu cgauaugcaa acucugaccc cuguggguag acuuaucaca gcgaauccag     1440

uuaucaccga aucuacugag aacagcaaga ugaugcugga guuggauccc ccuuuuggcg     1500

acucauauau ugugauaggg guaggagaga aaaagaucac gcaucacugg cauaggagug     1560

gcuccacaau ugguaaggcu uucgaagcca ccguccgggg cgcaaaacga auggccgugc     1620

ucggcgacac ugcuugggau uuuggaagcg uuggcggggc cuuaaauucu cugggaaagg     1680

guauccacca gauauucggc gcagcuuuca aaucacuguu ugggggaaug aguugguuca     1740

gccagauucu aaucggcacc cuccugaugu ggcuugguuu gaacgccaag aacgggucca     1800

ucucucugau gugccucgcg cugggcggag ugcuuauuuu ucugucgaca gccgucagcg     1860

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10479
<211>  2182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10479
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcugccgu uacccuuccc ucacacagua caaggaaacu gcagacucgg agccaaacgu      180

gguuggaguc ccgcgaauac accaagcauu uaauaaaagu ggagaacugg aucuuccgaa      240

auccugguuu ugcacuggua gccguggcua uugcauggcu acucgggucu ucgacaucac      300

agaaggucau cuaucuggug augauucuuc ugaucgcccc agcuuacagc auacguugua      360

ucgggguuag uaacagagac uucguggaag gaauguccgg cgguacuugg gucgaugugg      420

uauuggagca cgggggaugc guuaccguga uggcccagga caaaccgaca gucgauauug      480

aacucgugac cacuacagug ucuaauaugg cggaggucag gagcuauugu uacgaggcau      540

ccaucucaga cauggccagu gauagccggu gccccaccca gggcgaggcu uaucuggaca      600

agcaaucuga uacgcaguac guuuguaagc gcacucuggu ggacagaggc ugggggaacg      660

gaugcggucu cuuuggcaaa ggguccuuag uaaccugugc caaguucaca ugcagcaaaa      720

agaugacugg aaaaucaauu cagccugaaa aucuggaaua uaggaucaug cuuagugugc      780

auggcucuca gcacuccggu augauagggu acgagaccga ugaggaccgg gcaaaggucg      840

aagugacacc aaacagcccc cgcgcugagg ccaccuuggg aggcuuuggc ucgcuggggc      900

uagauuguga accucgaacg ggacucgacu ucucugaucu guacuaucuu acuaugaaua      960

acaagcauug gcugguucac aaagaguggu uucacgacau uccauugccc uggcaugcgg     1020

gugccgacac aggcaccccu cacuggaaua acaaggaagc acucguggag uucaaagaug     1080

cucaugccaa gagacaaacu gucgugguac ugggguccca ggagggagcu guucacacag     1140

ccuuagcagg cgcccuggaa gcugagaugg acggugcgaa agggaggcuc uuuucaggcc     1200

accuuaagug ccggcugaaa auggauaagu ugcgucuaaa gggagugagu uacagccugu     1260

guaccgcagc cuucacguuu accaaagucc cagcugaaac acuccauggc acugugaccg     1320

ucgaggugca guaugccggg acagacggac cgugcaagau ccccguucag auggcagugg     1380

auaugcaaac ucugaccccu guaggucgcc uuauuacagc caauccaguc aucaccgaau     1440

ccacugagaa cucuaaaaug augcuggagu uggauccccc uuucggcgac agcuacaucg     1500

ugauuggggu uggagacaag aaaauaacgc accauuggca cagaucaggc aguacaaucg     1560

guaaggcuuu ugaagccacc gugaggggcg caaagcggau ggcgguccuc ggcgauacug     1620

cuugggacuu cggauccgug ggcgggguau ucaauagccu gggaaaaggu auucaucaga     1680

ucuuuggcgc cgcauucaag ucuuuauuug ggggaauguc augguucagu cagauacuga     1740

uuggcacccu acucgugugg cugggucuua acaccaaaaa ugggagcauc uccuugacau     1800

gccuggcccu cggcggaguu augaucuuuc ugucuacugc ugucucggcc ugaggacuag     1860

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     1920

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     1980

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2040

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2160

ucuuuucaga gccaccagaa uu                                              2182


<210>  10480
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10480
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc aguggucauu gccugcgcgg      120

gagcugccgu uacccuuccc ucacacagua caaggaaacu gcagacucgg agccaaacgu      180

gguuggaguc ccgcgaauac accaagcauu uaauacgagu ggagaacugg aucuuccgua      240

auccugguuu ugcacuggcc gcugcagcca uugcuuggcu acucgggucu ucgacaucac      300

agaaaguaau cuaucuggug augauucuuc ugaucgcccc agcguacagc auaagaugua      360

ucggggucag uaacagggac uucguggaag gaauguccgg cgguacuugg guugaugugg      420

ucuuggagca cgggggaugc gugaccguaa uggcacagga caagccgaca guugauauug      480

aacucgugac cacuacaguc ucuaauaugg ccgaggugcg gagcuauugu uacgaggcuu      540

ccaucucaga cauggccagu gauagccgcu gccccaccca gggcgaagca uaucuggaca      600

aacaaucuga uacgcaguac guguguaaga gaacucuggu cgacaggggc ugggggaacg      660

gaugcggucu cuuuggcaag ggguccuuag uuaccugugc uaaauucgcc ugcucaaaga      720

aaaugacagg aaagagcauu cagccugaga aucuggaaua ucggaucaug cuuagugugc      780

auggcucuca gcacuccggu augauaguaa acgauacugg gcaugagacc gacgagaauc      840

gcgcgaaagu ggaaauuaca ccaaacagcc cccgagccga ggcaaccuug ggaggcuuug      900

gcucgcuggg gcuagauugu gaaccuagaa cgggacucga cuucucugau cuguacuacc      960

uuacuaugaa uaacaagcac uggcuggucc acaaggagug guuucaugac aucccauugc     1020

ccuggcacgc uggugccgac acaggcaccc cucauuggaa uaacaaagaa gcucucgugg     1080

aguucaagga ugcccacgca aaaaggcaaa cuguuguggu ccuggggucc caggagggag     1140

ccgugcacac agcuuuagcg ggcgcacugg aagccgagau ggacggugcu aaggggcggc     1200

ucucaagugg ccaucuuaaa ugccgucuga agauggauaa auugcgccua aagggaguaa     1260

gcuauucccu guguaccgcc gcauuuacgu ucaccaagau uccagccgaa acacuccacg     1320

gcacuguuac cguggagguc caguacgcug ggacagacgg accgugcaaa gugcccgccc     1380

agauggcagu cgauaugcaa acucugaccc cuguggguag acuuaucaca gcgaauccag     1440

uuaucaccga aucuacugag aacagcaaga ugaugcugga guuggauccc ccuuuuggcg     1500

acucauauau ugugauaggg guaggagaga aaaagaucac gcaucacugg cauaggagug     1560

gcuccacaau ugguaaggcu uucgaagcca ccguccgggg cgcaaaacga auggccgugc     1620

ucggcgacac ugcuugggau uuuggaagcg uuggcggggc cuuaaauucu cugggaaagg     1680

guauccacca gauauucggc gcagcuuuca aaucacuguu ugggggaaug aguugguuca     1740

gccagauucu aaucggcacc cuccugaugu ggcuugguuu gaacacaaag aacgggucca     1800

ucucucugau gugccucgcc cugggcggag ugcuuauuuu ucugucgacc gcggucagcg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10481
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10481
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1860

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1920

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     2040

ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc cuggcccugg     2100

gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10482
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10482
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caccugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1860

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1920

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     2040

ugauguggcu gggccugaac gccaagaacg gcagcaucag ccugaugugc cuggcccugg     2100

gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10483
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10483
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccau caccagaaga ggcagcgccu acuacaugua ccuggacaga agcgacgccg      180

gcaaggccau cagcuucgcc accacccugg gcgugaacaa gugccacgug cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau caagguggag aacuggaucu ucagaaaccc cggcuucgcc cugguggccg      540

uggccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucaccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucggcuacga gaccgacgag gacagagcca agguggaggu gacccccaac agccccagag     1140

ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc agaaccggcc     1200

uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga cagaccgugg     1380

uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg     1440

agauggacgg cgccaagggc agacuguuca gcggccaccu gaagugcaga cugaagaugg     1500

acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc accuucacca     1560

aggugcccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac gccggcaccg     1620

acggccccug caagaucccc gugcagaugg ccguggacau gcagacccug acccccgugg     1680

gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc aagaugaugc     1740

uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc gacaagaaga     1800

ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag gccaccguga     1860

gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc agcgugggcg     1920

gcguguucaa cagccugggc aagggcaucc accagaucuu cggcgccgcc uucaagagcc     1980

uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug guguggcugg     2040

gccugaacac caagaacggc agcaucagcc ugaccugccu ggcccugggc ggcgugauga     2100

ucuuccugag caccgccgug agcgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10484
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10484
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1860

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1920

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1980

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     2040

ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc cuggcccugg     2100

gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10485
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10485
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10486
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10486
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac accugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaacg     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10487
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10487
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cggcuacgag accgacgagg     1080

acagagccaa gguggaggug acccccaaca gccccagagc cgaggccacc cugggcggcu     1140

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu     1200

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1380

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1440

gacuguucag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1500

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aagauccccg     1620

ugcagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1680

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1740

gcgacagcua caucgugauc ggcgugggcg acaagaagau cacccaccac uggcacagaa     1800

gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag agaauggccg     1860

ugcugggcga caccgccugg gacuucggca gcgugggcgg cguguucaac agccugggca     1920

agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc augagcuggu     1980

ucagccagau ccugaucggc acccugcugg uguggcuggg ccugaacacc aagaacggca     2040

gcaucagccu gaccugccug gcccugggcg gcgugaugau cuuccugagc accgccguga     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10488
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10488
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc accaucggca aggccuucga ggccaccgug agaggcgcca     1860

agagaauggc cgugcugggc gacaccgccu gggacuucgg cagcgugggc ggcgcccuga     1920

acagccuggg caagggcauc caccagaucu ucggcgccgc cuucaagagc cuguucggcg     1980

gcaugagcug guucagccag auccugaucg gcacccugcu gauguggcug ggccugaaca     2040

ccaagaacgg cagcaucagc cugaugugcc uggcccuggg cggcgugcug aucuuccuga     2100

gcaccgccgu gagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10489
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10489
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag gccuucgagg     1860

ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg gacuucggca     1920

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2040

uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug gcccugggcg     2100

gcgugcugau cuuccugagc accgccguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10490
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10490
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacac cugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag gccuucgagg     1860

ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg gacuucggca     1920

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2040

uguggcuggg ccugaacgcc aagaacggca gcaucagccu gaugugccug gcccugggcg     2100

gcgugcugau cuuccugagc accgccguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10491
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10491
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccaucac cagaagaggc agcgccuacu acauguaccu ggacagaagc gacgccggca      180

aggccaucag cuucgccacc acccugggcg ugaacaagug ccacgugcag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucaa gguggagaac uggaucuuca gaaaccccgg cuucgcccug guggccgugg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc accugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

gcuacgagac cgacgaggac agagccaagg uggaggugac ccccaacagc cccagagccg     1140

aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga accggccugg     1200

acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag accguggugg     1380

ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug gaggccgaga     1440

uggacggcgc caagggcaga cuguucagcg gccaccugaa gugcagacug aagauggaca     1500

agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc uucaccaagg     1560

ugcccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg     1620

gccccugcaa gauccccgug cagauggccg uggacaugca gacccugacc cccgugggca     1680

gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag augaugcugg     1740

agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgac aagaagauca     1800

cccaccacug gcacagaagc ggcagcacca ucggcaaggc cuucgaggcc accgugagag     1860

gcgccaagag aauggccgug cugggcgaca ccgccuggga cuucggcagc gugggcggcg     1920

uguucaacag ccugggcaag ggcauccacc agaucuucgg cgccgccuuc aagagccugu     1980

ucggcggcau gagcugguuc agccagaucc ugaucggcac ccugcuggug uggcugggcc     2040

ugaacaccaa gaacggcagc aucagccuga ccugccuggc ccugggcggc gugaugaucu     2100

uccugagcac cgccgugagc gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10492
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10492
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac caucggcaag gccuucgagg     1860

ccaccgugag aggcgccaag agaauggccg ugcugggcga caccgccugg gacuucggca     1920

gcgugggcgg cgcccugaac agccugggca agggcaucca ccagaucuuc ggcgccgccu     1980

ucaagagccu guucggcggc augagcuggu ucagccagau ccugaucggc acccugcuga     2040

uguggcuggg ccugaacacc aagaacggca gcaucagccu gaugugccug gcccugggcg     2100

gcgugcugau cuuccugagc accgccguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10493
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10493
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag cacccugggc aaggccuuca     1860

gcaccacccu gaagggcgcc cagagacugg ccgcccuggg cgacaccgcc ugggacuucg     1920

gcagcaucgg cggcguguuc aacagcaucg gcagagccgu gcaccaggug uucggcggcg     1980

ccuucagaac ccuguucggc ggcaugagcu ggaucaccca gggccugaug ggcgcccugc     2040

ugcuguggau gggcgugaac gccagagaca gaagcaucgc ccuggccuuc cuggccaccg     2100

gcggcgugcu gguguuccug gccaccaacg ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10494
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10494
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caccugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag cacccugggc aaggccuuca     1860

gcaccacccu gaagggcgcc cagagacugg ccgcccuggg cgacaccgcc ugggacuucg     1920

gcagcaucgg cggcguguuc aacagcaucg gcagagccgu gcaccaggug uucggcggcg     1980

ccuucagaac ccuguucggc ggcaugagcu ggaucaccca gggccugaug ggcgcccugc     2040

ugcuguggau gggcgugaac gccagagaca gaagcaucgc ccuggccuuc cuggccaccg     2100

gcggcgugcu gguguuccug gccaccaacg ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10495
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10495
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccau caccagaaga ggcagcgccu acuacaugua ccuggacaga agcgacgccg      180

gcaaggccau cagcuucgcc accacccugg gcgugaacaa gugccacgug cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau caagguggag aacuggaucu ucagaaaccc cggcuucgcc cugguggccg      540

uggccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucaccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucggcuacga gaccgacgag gacagagcca agguggaggu gacccccaac agccccagag     1140

ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc agaaccggcc     1200

uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug gugcacaagg     1260

agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc accccccacu     1320

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga cagaccgugg     1380

uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg     1440

agauggacgg cgccaagggc agacuguuca gcggccaccu gaagugcaga cugaagaugg     1500

acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc accuucacca     1560

aggugcccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac gccggcaccg     1620

acggccccug caagaucccc gugcagaugg ccguggacau gcagacccug acccccgugg     1680

gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc aagaugaugc     1740

uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc gacaagaaga     1800

ucacccacca cuggcacaga agcggcagca cccugggcaa ggccuucagc accacccuga     1860

agggcgccca gagacuggcc gcccugggcg acaccgccug ggacuucggc agcaucggcg     1920

gcguguucaa cagcaucggc agagccgugc accagguguu cggcggcgcc uucagaaccc     1980

uguucggcgg caugagcugg aucacccagg gccugauggg cgcccugcug cuguggaugg     2040

gcgugaacgc cagagacaga agcaucgccc uggccuuccu ggccaccggc ggcgugcugg     2100

uguuccuggc caccaacgug cacgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10496
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10496
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgu gaccagaaga ggcagcgccu acuacaugua ccuggacaga aacgacgccg      180

gcgaggccau cagcuucccc accacccugg gcaugaacaa gugcuacauc cagaucaugg      240

accugggcca caugugcgac gccaccauga gcuacgagug ccccaugcug gacgagggcg      300

uggagcccga cgacguggac ugcuggugca acaccaccag caccugggug guguacggca      360

ccugccacca caagaagggc gaggccagaa gaagcagaag agccgugacc cugcccagcc      420

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      480

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      540

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      600

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      660

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      720

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      780

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      840

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      900

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      960

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc     1020

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga     1080

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca     1140

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc     1200

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc     1260

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1320

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1380

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1440

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1500

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1560

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1620

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1680

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1740

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1800

gcgagaagaa gaucacccac cacuggcaca gaagcggcag cacccugggc aaggccuuca     1860

gcaccacccu gaagggcgcc cagagacugg ccgcccuggg cgacaccgcc ugggacuucg     1920

gcagcaucgg cggcguguuc aacagcaucg gcagagccgu gcaccaggug uucggcggcg     1980

ccuucagaac ccuguucggc ggcaugagcu ggaucaccca gggccugaug ggcgcccugc     2040

ugcuguggau gggcgugaac gccagagaca gaagcaucgc ccuggccuuc cuggccaccg     2100

gcggcgugcu gguguuccug gccaccaacg ugcacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10497
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10497
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga caucgugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc acccugggca aggccuucag caccacccug aagggcgccc     1860

agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc ggcguguuca     1920

acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc cuguucggcg     1980

gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg     2040

ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10498
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10498
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac accugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc acccugggca aggccuucag caccacccug aagggcgccc     1860

agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc ggcguguuca     1920

acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc cuguucggcg     1980

gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg     2040

ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10499
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10499
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcauc accagaagag      120

gcagcgccua cuacauguac cuggacagaa gcgacgccgg caaggccauc agcuucgcca      180

ccacccuggg cgugaacaag ugccacgugc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc aagguggaga      480

acuggaucuu cagaaacccc ggcuucgccc ugguggccgu ggccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucaccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cggcuacgag accgacgagg     1080

acagagccaa gguggaggug acccccaaca gccccagagc cgaggccacc cugggcggcu     1140

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu     1200

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1260

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1320

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1380

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1440

gacuguucag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1500

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa ggugcccgcc gagacccugc     1560

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aagauccccg     1620

ugcagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1680

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1740

gcgacagcua caucgugauc ggcgugggcg acaagaagau cacccaccac uggcacagaa     1800

gcggcagcac ccugggcaag gccuucagca ccacccugaa gggcgcccag agacuggccg     1860

cccugggcga caccgccugg gacuucggca gcaucggcgg cguguucaac agcaucggca     1920

gagccgugca ccagguguuc ggcggcgccu ucagaacccu guucggcggc augagcugga     1980

ucacccaggg ccugaugggc gcccugcugc uguggauggg cgugaacgcc agagacagaa     2040

gcaucgcccu ggccuuccug gccaccggcg gcgugcuggu guuccuggcc accaacgugc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10500
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10500
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgug accagaagag      120

gcagcgccua cuacauguac cuggacagaa acgacgccgg cgaggccauc agcuucccca      180

ccacccuggg caugaacaag ugcuacaucc agaucaugga ccugggccac augugcgacg      240

ccaccaugag cuacgagugc cccaugcugg acgagggcgu ggagcccgac gacguggacu      300

gcuggugcaa caccaccagc accugggugg uguacggcac cugccaccac aagaagggcg      360

aggccagaag aagcagaaga gccgugaccc ugcccagcca cagcaccaga aagcugcaga      420

ccagaagcca gaccuggcug gagagcagag aguacaccaa gcaccugauc agaguggaga      480

acuggaucuu cagaaacccc ggcuucgccc uggccgccgc cgccaucgcc uggcugcugg      540

gcagcagcac cagccagaag gugaucuacc uggugaugau ccugcugauc gcccccgccu      600

acagcaucag augcaucggc gugagcaaca gagacuucgu ggagggcaug agcggcggca      660

ccugggugga cguggugcug gagcacggcg gcugcgugac cgugauggcc caggacaagc      720

ccaccgugga caucgagcug gugaccacca ccgugagcaa cauggccgag gugagaagcu      780

acugcuacga ggccagcauc agcgacaugg ccagcgacag cagaugcccc acccagggcg      840

aggccuaccu ggacaagcag agcgacaccc aguacgugug caagagaacc cugguggaca      900

gaggcugggg caacggcugc ggccuguucg gcaagggcag ccuggugacc ugcgccaagu      960

ucgccugcag caagaagaug accggcaaga gcauccagcc cgagaaccug gaguacagaa     1020

ucaugcugag cgugcacggc agccagcaca gcggcaugau cgugaacgac accggccacg     1080

agaccgacga gaacagagcc aagguggaga ucacccccaa cagccccaga gccgaggcca     1140

cccugggcgg cuucggcagc cugggccugg acugcgagcc cagaaccggc cuggacuuca     1200

gcgaccugua cuaccugacc augaacaaca agcacuggcu ggugcacaag gagugguucc     1260

acgacauccc ccugcccugg cacgccggcg ccgacaccgg caccccccac uggaacaaca     1320

aggaggcccu gguggaguuc aaggacgccc acgccaagag acagaccgug guggugcugg     1380

gcagccagga gggcgccgug cacaccgccc uggccggcgc ccuggaggcc gagauggacg     1440

gcgccaaggg cagacugagc agcggccacc ugaagugcag acugaagaug gacaagcuga     1500

gacugaaggg cgugagcuac agccugugca ccgccgccuu caccuucacc aagauccccg     1560

ccgagacccu gcacggcacc gugaccgugg aggugcagua cgccggcacc gacggccccu     1620

gcaaggugcc cgcccagaug gccguggaca ugcagacccu gacccccgug ggcagacuga     1680

ucaccgccaa ccccgugauc accgagagca ccgagaacag caagaugaug cuggagcugg     1740

acccccccuu cggcgacagc uacaucguga ucggcguggg cgagaagaag aucacccacc     1800

acuggcacag aagcggcagc acccugggca aggccuucag caccacccug aagggcgccc     1860

agagacuggc cgcccugggc gacaccgccu gggacuucgg cagcaucggc ggcguguuca     1920

acagcaucgg cagagccgug caccaggugu ucggcggcgc cuucagaacc cuguucggcg     1980

gcaugagcug gaucacccag ggccugaugg gcgcccugcu gcuguggaug ggcgugaacg     2040

ccagagacag aagcaucgcc cuggccuucc uggccaccgg cggcgugcug guguuccugg     2100

ccaccaacgu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10501
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10501
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggaca ucgugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac ccugggcaag gccuucagca     1860

ccacccugaa gggcgcccag agacuggccg cccugggcga caccgccugg gacuucggca     1920

gcaucggcgg cguguucaac agcaucggca gagccgugca ccagguguuc ggcggcgccu     1980

ucagaacccu guucggcggc augagcugga ucacccaggg ccugaugggc gcccugcugc     2040

uguggauggg cgugaacgcc agagacagaa gcaucgcccu ggccuuccug gccaccggcg     2100

gcgugcuggu guuccuggcc accaacgugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10502
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10502
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacac cugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac ccugggcaag gccuucagca     1860

ccacccugaa gggcgcccag agacuggccg cccugggcga caccgccugg gacuucggca     1920

gcaucggcgg cguguucaac agcaucggca gagccgugca ccagguguuc ggcggcgccu     1980

ucagaacccu guucggcggc augagcugga ucacccaggg ccugaugggc gcccugcugc     2040

uguggauggg cgugaacgcc agagacagaa gcaucgcccu ggccuuccug gccaccggcg     2100

gcgugcuggu guuccuggcc accaacgugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10503
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10503
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccaucac cagaagaggc agcgccuacu acauguaccu ggacagaagc gacgccggca      180

aggccaucag cuucgccacc acccugggcg ugaacaagug ccacgugcag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucaa gguggagaac uggaucuuca gaaaccccgg cuucgcccug guggccgugg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc accugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

gcuacgagac cgacgaggac agagccaagg uggaggugac ccccaacagc cccagagccg     1140

aggccacccu gggcggcuuc ggcagccugg gccuggacug cgagcccaga accggccugg     1200

acuucagcga ccuguacuac cugaccauga acaacaagca cuggcuggug cacaaggagu     1260

gguuccacga caucccccug cccuggcacg ccggcgccga caccggcacc ccccacugga     1320

acaacaagga ggcccuggug gaguucaagg acgcccacgc caagagacag accguggugg     1380

ugcugggcag ccaggagggc gccgugcaca ccgcccuggc cggcgcccug gaggccgaga     1440

uggacggcgc caagggcaga cuguucagcg gccaccugaa gugcagacug aagauggaca     1500

agcugagacu gaagggcgug agcuacagcc ugugcaccgc cgccuucacc uucaccaagg     1560

ugcccgccga gacccugcac ggcaccguga ccguggaggu gcaguacgcc ggcaccgacg     1620

gccccugcaa gauccccgug cagauggccg uggacaugca gacccugacc cccgugggca     1680

gacugaucac cgccaacccc gugaucaccg agagcaccga gaacagcaag augaugcugg     1740

agcuggaccc ccccuucggc gacagcuaca ucgugaucgg cgugggcgac aagaagauca     1800

cccaccacug gcacagaagc ggcagcaccc ugggcaaggc cuucagcacc acccugaagg     1860

gcgcccagag acuggccgcc cugggcgaca ccgccuggga cuucggcagc aucggcggcg     1920

uguucaacag caucggcaga gccgugcacc agguguucgg cggcgccuuc agaacccugu     1980

ucggcggcau gagcuggauc acccagggcc ugaugggcgc ccugcugcug uggaugggcg     2040

ugaacgccag agacagaagc aucgcccugg ccuuccuggc caccggcggc gugcuggugu     2100

uccuggccac caacgugcac gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10504
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10504
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgugac cagaagaggc agcgccuacu acauguaccu ggacagaaac gacgccggcg      180

aggccaucag cuuccccacc acccugggca ugaacaagug cuacauccag aucauggacc      240

ugggccacau gugcgacgcc accaugagcu acgagugccc caugcuggac gagggcgugg      300

agcccgacga cguggacugc uggugcaaca ccaccagcac cuggguggug uacggcaccu      360

gccaccacaa gaagggcgag gccagaagaa gcagaagagc cgugacccug cccagccaca      420

gcaccagaaa gcugcagacc agaagccaga ccuggcugga gagcagagag uacaccaagc      480

accugaucag aguggagaac uggaucuuca gaaaccccgg cuucgcccug gccgccgccg      540

ccaucgccug gcugcugggc agcagcacca gccagaaggu gaucuaccug gugaugaucc      600

ugcugaucgc ccccgccuac agcaucagau gcaucggcgu gagcaacaga gacuucgugg      660

agggcaugag cggcggcacc uggguggacg uggugcugga gcacggcggc ugcgugaccg      720

ugauggccca ggacaagccc accguggaca ucgagcuggu gaccaccacc gugagcaaca      780

uggccgaggu gagaagcuac ugcuacgagg ccagcaucag cgacauggcc agcgacagca      840

gaugccccac ccagggcgag gccuaccugg acaagcagag cgacacccag uacgugugca      900

agagaacccu gguggacaga ggcuggggca acggcugcgg ccuguucggc aagggcagcc      960

uggugaccug cgccaaguuc gccugcagca agaagaugac cggcaagagc auccagcccg     1020

agaaccugga guacagaauc augcugagcg ugcacggcag ccagcacagc ggcaugaucg     1080

ugaacgacac cggccacgag accgacgaga acagagccaa gguggagauc acccccaaca     1140

gccccagagc cgaggccacc cugggcggcu ucggcagccu gggccuggac ugcgagccca     1200

gaaccggccu ggacuucagc gaccuguacu accugaccau gaacaacaag cacuggcugg     1260

ugcacaagga gugguuccac gacauccccc ugcccuggca cgccggcgcc gacaccggca     1320

ccccccacug gaacaacaag gaggcccugg uggaguucaa ggacgcccac gccaagagac     1380

agaccguggu ggugcugggc agccaggagg gcgccgugca caccgcccug gccggcgccc     1440

uggaggccga gauggacggc gccaagggca gacugagcag cggccaccug aagugcagac     1500

ugaagaugga caagcugaga cugaagggcg ugagcuacag ccugugcacc gccgccuuca     1560

ccuucaccaa gauccccgcc gagacccugc acggcaccgu gaccguggag gugcaguacg     1620

ccggcaccga cggccccugc aaggugcccg cccagauggc cguggacaug cagacccuga     1680

cccccguggg cagacugauc accgccaacc ccgugaucac cgagagcacc gagaacagca     1740

agaugaugcu ggagcuggac ccccccuucg gcgacagcua caucgugauc ggcgugggcg     1800

agaagaagau cacccaccac uggcacagaa gcggcagcac ccugggcaag gccuucagca     1860

ccacccugaa gggcgcccag agacuggccg cccugggcga caccgccugg gacuucggca     1920

gcaucggcgg cguguucaac agcaucggca gagccgugca ccagguguuc ggcggcgccu     1980

ucagaacccu guucggcggc augagcugga ucacccaggg ccugaugggc gcccugcugc     2040

uguggauggg cgugaacgcc agagacagaa gcaucgcccu ggccuuccug gccaccggcg     2100

gcgugcuggu guuccuggcc accaacgugc acgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10505
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10505
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgugacc cugcccagcc      120

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      180

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      240

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      300

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      360

uggagggcau gagcggcggc accugggugg acaucgugcu ggagcacggc ggcugcguga      420

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      480

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      540

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      600

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      660

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc      720

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga      780

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca      840

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc      900

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc      960

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1020

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1080

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1140

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1200

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1260

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1320

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1380

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1440

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1500

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1560

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1620

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1680

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     1740

ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc cuggcccugg     1800

gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10506
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10506
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgugacc cugcccagcc      120

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      180

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      240

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      300

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      360

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      420

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      480

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      540

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      600

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      660

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc      720

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga      780

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca      840

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc      900

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc      960

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1020

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1080

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1140

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1200

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1260

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1320

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1380

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1440

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1500

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1560

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1620

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1680

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     1740

ugauguggcu gggccugaac gccaagaacg gcagcaucag ccugaugugc cuggcccugg     1800

gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10507
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10507
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau caucggccug cugcugacca ccgccauggc cgccgugacc cugcccagcc      120

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      180

agcaccugau caagguggag aacuggaucu ucagaaaccc cggcuucgcc cugguggccg      240

uggccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      300

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      360

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      420

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      480

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      540

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      600

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      660

gccuggugac cugcgccaag uucaccugca gcaagaagau gaccggcaag agcauccagc      720

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga      780

ucggcuacga gaccgacgag gacagagcca agguggaggu gacccccaac agccccagag      840

ccgaggccac ccugggcggc uucggcagcc ugggccugga cugcgagccc agaaccggcc      900

uggacuucag cgaccuguac uaccugacca ugaacaacaa gcacuggcug gugcacaagg      960

agugguucca cgacaucccc cugcccuggc acgccggcgc cgacaccggc accccccacu     1020

ggaacaacaa ggaggcccug guggaguuca aggacgccca cgccaagaga cagaccgugg     1080

uggugcuggg cagccaggag ggcgccgugc acaccgcccu ggccggcgcc cuggaggccg     1140

agauggacgg cgccaagggc agacuguuca gcggccaccu gaagugcaga cugaagaugg     1200

acaagcugag acugaagggc gugagcuaca gccugugcac cgccgccuuc accuucacca     1260

aggugcccgc cgagacccug cacggcaccg ugaccgugga ggugcaguac gccggcaccg     1320

acggccccug caagaucccc gugcagaugg ccguggacau gcagacccug acccccgugg     1380

gcagacugau caccgccaac cccgugauca ccgagagcac cgagaacagc aagaugaugc     1440

uggagcugga cccccccuuc ggcgacagcu acaucgugau cggcgugggc gacaagaaga     1500

ucacccacca cuggcacaga agcggcagca ccaucggcaa ggccuucgag gccaccguga     1560

gaggcgccaa gagaauggcc gugcugggcg acaccgccug ggacuucggc agcgugggcg     1620

gcguguucaa cagccugggc aagggcaucc accagaucuu cggcgccgcc uucaagagcc     1680

uguucggcgg caugagcugg uucagccaga uccugaucgg cacccugcug guguggcugg     1740

gccugaacac caagaacggc agcaucagcc ugaccugccu ggcccugggc ggcgugauga     1800

ucuuccugag caccgccgug agcgccugag gacuagugca ucacauuuaa aagcaucuca     1860

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     1920

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     1980

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2100

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2158


<210>  10508
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10508
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgugggccug cugcugacca ccgccauggc cgccgugacc cugcccagcc      120

acagcaccag aaagcugcag accagaagcc agaccuggcu ggagagcaga gaguacacca      180

agcaccugau cagaguggag aacuggaucu ucagaaaccc cggcuucgcc cuggccgccg      240

ccgccaucgc cuggcugcug ggcagcagca ccagccagaa ggugaucuac cuggugauga      300

uccugcugau cgcccccgcc uacagcauca gaugcaucgg cgugagcaac agagacuucg      360

uggagggcau gagcggcggc accugggugg acguggugcu ggagcacggc ggcugcguga      420

ccgugauggc ccaggacaag cccaccgugg acaucgagcu ggugaccacc accgugagca      480

acauggccga ggugagaagc uacugcuacg aggccagcau cagcgacaug gccagcgaca      540

gcagaugccc cacccagggc gaggccuacc uggacaagca gagcgacacc caguacgugu      600

gcaagagaac ccugguggac agaggcuggg gcaacggcug cggccuguuc ggcaagggca      660

gccuggugac cugcgccaag uucgccugca gcaagaagau gaccggcaag agcauccagc      720

ccgagaaccu ggaguacaga aucaugcuga gcgugcacgg cagccagcac agcggcauga      780

ucgugaacga caccggccac gagaccgacg agaacagagc caagguggag aucaccccca      840

acagccccag agccgaggcc acccugggcg gcuucggcag ccugggccug gacugcgagc      900

ccagaaccgg ccuggacuuc agcgaccugu acuaccugac caugaacaac aagcacuggc      960

uggugcacaa ggagugguuc cacgacaucc cccugcccug gcacgccggc gccgacaccg     1020

gcacccccca cuggaacaac aaggaggccc ugguggaguu caaggacgcc cacgccaaga     1080

gacagaccgu gguggugcug ggcagccagg agggcgccgu gcacaccgcc cuggccggcg     1140

cccuggaggc cgagauggac ggcgccaagg gcagacugag cagcggccac cugaagugca     1200

gacugaagau ggacaagcug agacugaagg gcgugagcua cagccugugc accgccgccu     1260

ucaccuucac caagaucccc gccgagaccc ugcacggcac cgugaccgug gaggugcagu     1320

acgccggcac cgacggcccc ugcaaggugc ccgcccagau ggccguggac augcagaccc     1380

ugacccccgu gggcagacug aucaccgcca accccgugau caccgagagc accgagaaca     1440

gcaagaugau gcuggagcug gacccccccu ucggcgacag cuacaucgug aucggcgugg     1500

gcgagaagaa gaucacccac cacuggcaca gaagcggcag caccaucggc aaggccuucg     1560

aggccaccgu gagaggcgcc aagagaaugg ccgugcuggg cgacaccgcc ugggacuucg     1620

gcagcguggg cggcgcccug aacagccugg gcaagggcau ccaccagauc uucggcgccg     1680

ccuucaagag ccuguucggc ggcaugagcu gguucagcca gauccugauc ggcacccugc     1740

ugauguggcu gggccugaac accaagaacg gcagcaucag ccugaugugc cuggcccugg     1800

gcggcgugcu gaucuuccug agcaccgccg ugagcgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10509
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10509
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgc cgugacccug cccagccaca gcaccagaaa gcugcagacc agaagccaga      180

ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac uggaucuuca      240

gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc agcagcacca      300

gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac agcaucagau      360

gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc uggguggaca      420

ucgugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc accguggaca      480

ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac ugcuacgagg      540

ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag gccuaccugg      600

acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga ggcuggggca      660

acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc gccugcagca      720

agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc augcugagcg      780

ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag accgacgaga      840

acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc cugggcggcu      900

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu      960

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1020

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1080

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1140

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1200

gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1260

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc gagacccugc     1320

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aaggugcccg     1380

cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1440

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1500

gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac uggcacagaa     1560

gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag agaauggccg     1620

ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac agccugggca     1680

agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc augagcuggu     1740

ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc aagaacggca     1800

gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc accgccguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10510
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10510
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgc cgugacccug cccagccaca gcaccagaaa gcugcagacc agaagccaga      180

ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac uggaucuuca      240

gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc agcagcacca      300

gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac agcaucagau      360

gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc uggguggacg      420

uggugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc accguggaca      480

ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac ugcuacgagg      540

ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag gccuaccugg      600

acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga ggcuggggca      660

acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc gccugcagca      720

agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc augcugagcg      780

ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag accgacgaga      840

acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc cugggcggcu      900

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu      960

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1020

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1080

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1140

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1200

gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1260

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc gagacccugc     1320

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aaggugcccg     1380

cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1440

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1500

gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac uggcacagaa     1560

gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag agaauggccg     1620

ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac agccugggca     1680

agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc augagcuggu     1740

ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacgcc aagaacggca     1800

gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc accgccguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10511
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10511
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgc cgugacccug cccagccaca gcaccagaaa gcugcagacc agaagccaga      180

ccuggcugga gagcagagag uacaccaagc accugaucaa gguggagaac uggaucuuca      240

gaaaccccgg cuucgcccug guggccgugg ccaucgccug gcugcugggc agcagcacca      300

gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac agcaucagau      360

gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc uggguggacg      420

uggugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc accguggaca      480

ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac ugcuacgagg      540

ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag gccuaccugg      600

acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga ggcuggggca      660

acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc accugcagca      720

agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc augcugagcg      780

ugcacggcag ccagcacagc ggcaugaucg gcuacgagac cgacgaggac agagccaagg      840

uggaggugac ccccaacagc cccagagccg aggccacccu gggcggcuuc ggcagccugg      900

gccuggacug cgagcccaga accggccugg acuucagcga ccuguacuac cugaccauga      960

acaacaagca cuggcuggug cacaaggagu gguuccacga caucccccug cccuggcacg     1020

ccggcgccga caccggcacc ccccacugga acaacaagga ggcccuggug gaguucaagg     1080

acgcccacgc caagagacag accguggugg ugcugggcag ccaggagggc gccgugcaca     1140

ccgcccuggc cggcgcccug gaggccgaga uggacggcgc caagggcaga cuguucagcg     1200

gccaccugaa gugcagacug aagauggaca agcugagacu gaagggcgug agcuacagcc     1260

ugugcaccgc cgccuucacc uucaccaagg ugcccgccga gacccugcac ggcaccguga     1320

ccguggaggu gcaguacgcc ggcaccgacg gccccugcaa gauccccgug cagauggccg     1380

uggacaugca gacccugacc cccgugggca gacugaucac cgccaacccc gugaucaccg     1440

agagcaccga gaacagcaag augaugcugg agcuggaccc ccccuucggc gacagcuaca     1500

ucgugaucgg cgugggcgac aagaagauca cccaccacug gcacagaagc ggcagcacca     1560

ucggcaaggc cuucgaggcc accgugagag gcgccaagag aauggccgug cugggcgaca     1620

ccgccuggga cuucggcagc gugggcggcg uguucaacag ccugggcaag ggcauccacc     1680

agaucuucgg cgccgccuuc aagagccugu ucggcggcau gagcugguuc agccagaucc     1740

ugaucggcac ccugcuggug uggcugggcc ugaacaccaa gaacggcagc aucagccuga     1800

ccugccuggc ccugggcggc gugaugaucu uccugagcac cgccgugagc gccugaggac     1860

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     1920

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     1980

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2040

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2160

ggcucuuuuc agagccacca gaauu                                           2185


<210>  10512
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10512
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccgugcug ggcuucuuca ucaucgccgu gcugaugagc gcccaggaga      120

gcugggccgc cgugacccug cccagccaca gcaccagaaa gcugcagacc agaagccaga      180

ccuggcugga gagcagagag uacaccaagc accugaucag aguggagaac uggaucuuca      240

gaaaccccgg cuucgcccug gccgccgccg ccaucgccug gcugcugggc agcagcacca      300

gccagaaggu gaucuaccug gugaugaucc ugcugaucgc ccccgccuac agcaucagau      360

gcaucggcgu gagcaacaga gacuucgugg agggcaugag cggcggcacc uggguggacg      420

uggugcugga gcacggcggc ugcgugaccg ugauggccca ggacaagccc accguggaca      480

ucgagcuggu gaccaccacc gugagcaaca uggccgaggu gagaagcuac ugcuacgagg      540

ccagcaucag cgacauggcc agcgacagca gaugccccac ccagggcgag gccuaccugg      600

acaagcagag cgacacccag uacgugugca agagaacccu gguggacaga ggcuggggca      660

acggcugcgg ccuguucggc aagggcagcc uggugaccug cgccaaguuc gccugcagca      720

agaagaugac cggcaagagc auccagcccg agaaccugga guacagaauc augcugagcg      780

ugcacggcag ccagcacagc ggcaugaucg ugaacgacac cggccacgag accgacgaga      840

acagagccaa gguggagauc acccccaaca gccccagagc cgaggccacc cugggcggcu      900

ucggcagccu gggccuggac ugcgagccca gaaccggccu ggacuucagc gaccuguacu      960

accugaccau gaacaacaag cacuggcugg ugcacaagga gugguuccac gacauccccc     1020

ugcccuggca cgccggcgcc gacaccggca ccccccacug gaacaacaag gaggcccugg     1080

uggaguucaa ggacgcccac gccaagagac agaccguggu ggugcugggc agccaggagg     1140

gcgccgugca caccgcccug gccggcgccc uggaggccga gauggacggc gccaagggca     1200

gacugagcag cggccaccug aagugcagac ugaagaugga caagcugaga cugaagggcg     1260

ugagcuacag ccugugcacc gccgccuuca ccuucaccaa gauccccgcc gagacccugc     1320

acggcaccgu gaccguggag gugcaguacg ccggcaccga cggccccugc aaggugcccg     1380

cccagauggc cguggacaug cagacccuga cccccguggg cagacugauc accgccaacc     1440

ccgugaucac cgagagcacc gagaacagca agaugaugcu ggagcuggac ccccccuucg     1500

gcgacagcua caucgugauc ggcgugggcg agaagaagau cacccaccac uggcacagaa     1560

gcggcagcac caucggcaag gccuucgagg ccaccgugag aggcgccaag agaauggccg     1620

ugcugggcga caccgccugg gacuucggca gcgugggcgg cgcccugaac agccugggca     1680

agggcaucca ccagaucuuc ggcgccgccu ucaagagccu guucggcggc augagcuggu     1740

ucagccagau ccugaucggc acccugcuga uguggcuggg ccugaacacc aagaacggca     1800

gcaucagccu gaugugccug gcccugggcg gcgugcugau cuuccugagc accgccguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10513
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10513
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgcc gugacccugc      120

ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag agcagagagu      180

acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc uucgcccugg      240

ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug aucuaccugg      300

ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug agcaacagag      360

acuucgugga gggcaugagc ggcggcaccu ggguggacau cgugcuggag cacggcggcu      420

gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug accaccaccg      480

ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc gacauggcca      540

gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc gacacccagu      600

acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc cuguucggca      660

agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc ggcaagagca      720

uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc cagcacagcg      780

gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag guggagauca      840

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu      900

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc      960

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1020

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1080

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1140

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga     1200

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1260

ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug accguggagg     1320

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1380

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1440

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1500

gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1560

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1620

acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac cagaucuucg     1680

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     1740

cccugcugau guggcugggc cugaacacca agaacggcag caucagccug augugccugg     1800

cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10514
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10514
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgcc gugacccugc      120

ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag agcagagagu      180

acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc uucgcccugg      240

ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug aucuaccugg      300

ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug agcaacagag      360

acuucgugga gggcaugagc ggcggcaccu ggguggacgu ggugcuggag cacggcggcu      420

gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug accaccaccg      480

ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc gacauggcca      540

gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc gacacccagu      600

acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc cuguucggca      660

agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc ggcaagagca      720

uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc cagcacagcg      780

gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag guggagauca      840

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu      900

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc      960

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1020

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1080

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1140

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga     1200

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1260

ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug accguggagg     1320

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1380

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1440

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1500

gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1560

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1620

acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac cagaucuucg     1680

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     1740

cccugcugau guggcugggc cugaacgcca agaacggcag caucagccug augugccugg     1800

cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10515
<211>  2164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10515
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgcc gugacccugc      120

ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag agcagagagu      180

acaccaagca ccugaucaag guggagaacu ggaucuucag aaaccccggc uucgcccugg      240

uggccguggc caucgccugg cugcugggca gcagcaccag ccagaaggug aucuaccugg      300

ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug agcaacagag      360

acuucgugga gggcaugagc ggcggcaccu ggguggacgu ggugcuggag cacggcggcu      420

gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug accaccaccg      480

ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc gacauggcca      540

gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc gacacccagu      600

acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc cuguucggca      660

agggcagccu ggugaccugc gccaaguuca ccugcagcaa gaagaugacc ggcaagagca      720

uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc cagcacagcg      780

gcaugaucgg cuacgagacc gacgaggaca gagccaaggu ggaggugacc cccaacagcc      840

ccagagccga ggccacccug ggcggcuucg gcagccuggg ccuggacugc gagcccagaa      900

ccggccugga cuucagcgac cuguacuacc ugaccaugaa caacaagcac uggcuggugc      960

acaaggagug guuccacgac aucccccugc ccuggcacgc cggcgccgac accggcaccc     1020

cccacuggaa caacaaggag gcccuggugg aguucaagga cgcccacgcc aagagacaga     1080

ccgugguggu gcugggcagc caggagggcg ccgugcacac cgcccuggcc ggcgcccugg     1140

aggccgagau ggacggcgcc aagggcagac uguucagcgg ccaccugaag ugcagacuga     1200

agauggacaa gcugagacug aagggcguga gcuacagccu gugcaccgcc gccuucaccu     1260

ucaccaaggu gcccgccgag acccugcacg gcaccgugac cguggaggug caguacgccg     1320

gcaccgacgg ccccugcaag auccccgugc agauggccgu ggacaugcag acccugaccc     1380

ccgugggcag acugaucacc gccaaccccg ugaucaccga gagcaccgag aacagcaaga     1440

ugaugcugga gcuggacccc cccuucggcg acagcuacau cgugaucggc gugggcgaca     1500

agaagaucac ccaccacugg cacagaagcg gcagcaccau cggcaaggcc uucgaggcca     1560

ccgugagagg cgccaagaga auggccgugc ugggcgacac cgccugggac uucggcagcg     1620

ugggcggcgu guucaacagc cugggcaagg gcauccacca gaucuucggc gccgccuuca     1680

agagccuguu cggcggcaug agcugguuca gccagauccu gaucggcacc cugcuggugu     1740

ggcugggccu gaacaccaag aacggcagca ucagccugac cugccuggcc cugggcggcg     1800

ugaugaucuu ccugagcacc gccgugagcg ccugaggacu agugcaucac auuuaaaagc     1860

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     1920

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     1980

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2160

aauu                                                                  2164


<210>  10516
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10516
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccug guggccgccg ccaccagagu gcacagcgcc gugacccugc      120

ccagccacag caccagaaag cugcagacca gaagccagac cuggcuggag agcagagagu      180

acaccaagca ccugaucaga guggagaacu ggaucuucag aaaccccggc uucgcccugg      240

ccgccgccgc caucgccugg cugcugggca gcagcaccag ccagaaggug aucuaccugg      300

ugaugauccu gcugaucgcc cccgccuaca gcaucagaug caucggcgug agcaacagag      360

acuucgugga gggcaugagc ggcggcaccu ggguggacgu ggugcuggag cacggcggcu      420

gcgugaccgu gauggcccag gacaagccca ccguggacau cgagcuggug accaccaccg      480

ugagcaacau ggccgaggug agaagcuacu gcuacgaggc cagcaucagc gacauggcca      540

gcgacagcag augccccacc cagggcgagg ccuaccugga caagcagagc gacacccagu      600

acgugugcaa gagaacccug guggacagag gcuggggcaa cggcugcggc cuguucggca      660

agggcagccu ggugaccugc gccaaguucg ccugcagcaa gaagaugacc ggcaagagca      720

uccagcccga gaaccuggag uacagaauca ugcugagcgu gcacggcagc cagcacagcg      780

gcaugaucgu gaacgacacc ggccacgaga ccgacgagaa cagagccaag guggagauca      840

cccccaacag ccccagagcc gaggccaccc ugggcggcuu cggcagccug ggccuggacu      900

gcgagcccag aaccggccug gacuucagcg accuguacua ccugaccaug aacaacaagc      960

acuggcuggu gcacaaggag ugguuccacg acaucccccu gcccuggcac gccggcgccg     1020

acaccggcac cccccacugg aacaacaagg aggcccuggu ggaguucaag gacgcccacg     1080

ccaagagaca gaccguggug gugcugggca gccaggaggg cgccgugcac accgcccugg     1140

ccggcgcccu ggaggccgag auggacggcg ccaagggcag acugagcagc ggccaccuga     1200

agugcagacu gaagauggac aagcugagac ugaagggcgu gagcuacagc cugugcaccg     1260

ccgccuucac cuucaccaag auccccgccg agacccugca cggcaccgug accguggagg     1320

ugcaguacgc cggcaccgac ggccccugca aggugcccgc ccagauggcc guggacaugc     1380

agacccugac ccccgugggc agacugauca ccgccaaccc cgugaucacc gagagcaccg     1440

agaacagcaa gaugaugcug gagcuggacc cccccuucgg cgacagcuac aucgugaucg     1500

gcgugggcga gaagaagauc acccaccacu ggcacagaag cggcagcacc aucggcaagg     1560

ccuucgaggc caccgugaga ggcgccaaga gaauggccgu gcugggcgac accgccuggg     1620

acuucggcag cgugggcggc gcccugaaca gccugggcaa gggcauccac cagaucuucg     1680

gcgccgccuu caagagccug uucggcggca ugagcugguu cagccagauc cugaucggca     1740

cccugcugau guggcugggc cugaacacca agaacggcag caucagccug augugccugg     1800

cccugggcgg cgugcugauc uuccugagca ccgccgugag cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10517
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10517
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      180

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      240

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      300

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      360

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacaucg      420

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      480

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      540

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      600

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      660

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      720

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      780

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      840

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      900

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      960

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1020

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1080

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1140

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1200

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1260

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1320

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1380

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1440

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1500

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1560

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1620

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1680

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     1740

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     1800

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10518
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10518
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      180

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      240

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      300

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      360

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      420

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      480

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      540

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      600

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      660

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      720

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      780

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      840

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      900

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      960

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1020

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1080

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1140

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1200

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1260

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1320

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1380

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1440

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1500

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1560

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1620

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1680

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     1740

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacgccaag aacggcagca     1800

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10519
<211>  2182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10519
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      180

ggcuggagag cagagaguac accaagcacc ugaucaaggu ggagaacugg aucuucagaa      240

accccggcuu cgcccuggug gccguggcca ucgccuggcu gcugggcagc agcaccagcc      300

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      360

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      420

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      480

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      540

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      600

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      660

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucacc ugcagcaaga      720

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      780

acggcagcca gcacagcggc augaucggcu acgagaccga cgaggacaga gccaaggugg      840

aggugacccc caacagcccc agagccgagg ccacccuggg cggcuucggc agccugggcc      900

uggacugcga gcccagaacc ggccuggacu ucagcgaccu guacuaccug accaugaaca      960

acaagcacug gcuggugcac aaggaguggu uccacgacau cccccugccc uggcacgccg     1020

gcgccgacac cggcaccccc cacuggaaca acaaggaggc ccugguggag uucaaggacg     1080

cccacgccaa gagacagacc gugguggugc ugggcagcca ggagggcgcc gugcacaccg     1140

cccuggccgg cgcccuggag gccgagaugg acggcgccaa gggcagacug uucagcggcc     1200

accugaagug cagacugaag auggacaagc ugagacugaa gggcgugagc uacagccugu     1260

gcaccgccgc cuucaccuuc accaaggugc ccgccgagac ccugcacggc accgugaccg     1320

uggaggugca guacgccggc accgacggcc ccugcaagau ccccgugcag auggccgugg     1380

acaugcagac ccugaccccc gugggcagac ugaucaccgc caaccccgug aucaccgaga     1440

gcaccgagaa cagcaagaug augcuggagc uggacccccc cuucggcgac agcuacaucg     1500

ugaucggcgu gggcgacaag aagaucaccc accacuggca cagaagcggc agcaccaucg     1560

gcaaggccuu cgaggccacc gugagaggcg ccaagagaau ggccgugcug ggcgacaccg     1620

ccugggacuu cggcagcgug ggcggcgugu ucaacagccu gggcaagggc auccaccaga     1680

ucuucggcgc cgccuucaag agccuguucg gcggcaugag cugguucagc cagauccuga     1740

ucggcacccu gcuggugugg cugggccuga acaccaagaa cggcagcauc agccugaccu     1800

gccuggcccu gggcggcgug augaucuucc ugagcaccgc cgugagcgcc ugaggacuag     1860

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     1920

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     1980

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2040

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2160

ucuuuucaga gccaccagaa uu                                              2182


<210>  10520
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10520
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggcagcauc auguggcugg ccagccuggc cguggugauc gccugcgccg      120

gcgccgccgu gacccugccc agccacagca ccagaaagcu gcagaccaga agccagaccu      180

ggcuggagag cagagaguac accaagcacc ugaucagagu ggagaacugg aucuucagaa      240

accccggcuu cgcccuggcc gccgccgcca ucgccuggcu gcugggcagc agcaccagcc      300

agaaggugau cuaccuggug augauccugc ugaucgcccc cgccuacagc aucagaugca      360

ucggcgugag caacagagac uucguggagg gcaugagcgg cggcaccugg guggacgugg      420

ugcuggagca cggcggcugc gugaccguga uggcccagga caagcccacc guggacaucg      480

agcuggugac caccaccgug agcaacaugg ccgaggugag aagcuacugc uacgaggcca      540

gcaucagcga cauggccagc gacagcagau gccccaccca gggcgaggcc uaccuggaca      600

agcagagcga cacccaguac gugugcaaga gaacccuggu ggacagaggc uggggcaacg      660

gcugcggccu guucggcaag ggcagccugg ugaccugcgc caaguucgcc ugcagcaaga      720

agaugaccgg caagagcauc cagcccgaga accuggagua cagaaucaug cugagcgugc      780

acggcagcca gcacagcggc augaucguga acgacaccgg ccacgagacc gacgagaaca      840

gagccaaggu ggagaucacc cccaacagcc ccagagccga ggccacccug ggcggcuucg      900

gcagccuggg ccuggacugc gagcccagaa ccggccugga cuucagcgac cuguacuacc      960

ugaccaugaa caacaagcac uggcuggugc acaaggagug guuccacgac aucccccugc     1020

ccuggcacgc cggcgccgac accggcaccc cccacuggaa caacaaggag gcccuggugg     1080

aguucaagga cgcccacgcc aagagacaga ccgugguggu gcugggcagc caggagggcg     1140

ccgugcacac cgcccuggcc ggcgcccugg aggccgagau ggacggcgcc aagggcagac     1200

ugagcagcgg ccaccugaag ugcagacuga agauggacaa gcugagacug aagggcguga     1260

gcuacagccu gugcaccgcc gccuucaccu ucaccaagau ccccgccgag acccugcacg     1320

gcaccgugac cguggaggug caguacgccg gcaccgacgg ccccugcaag gugcccgccc     1380

agauggccgu ggacaugcag acccugaccc ccgugggcag acugaucacc gccaaccccg     1440

ugaucaccga gagcaccgag aacagcaaga ugaugcugga gcuggacccc cccuucggcg     1500

acagcuacau cgugaucggc gugggcgaga agaagaucac ccaccacugg cacagaagcg     1560

gcagcaccau cggcaaggcc uucgaggcca ccgugagagg cgccaagaga auggccgugc     1620

ugggcgacac cgccugggac uucggcagcg ugggcggcgc ccugaacagc cugggcaagg     1680

gcauccacca gaucuucggc gccgccuuca agagccuguu cggcggcaug agcugguuca     1740

gccagauccu gaucggcacc cugcugaugu ggcugggccu gaacaccaag aacggcagca     1800

ucagccugau gugccuggcc cugggcggcg ugcugaucuu ccugagcacc gccgugagcg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10521
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10521
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggu gaccagacgg ggaagcgcuu acuauaugua ccuggaccgc aacgaugccg      180

gcgaagccau uuccuucccu acaacgcuug ggaugaauaa gugcuauauc caaauuaugg      240

accugggaca caugugugau gcuaccaugu cuuacgagug cccgaugcuc gacgaaggcg      300

uggagcccga ugacgucgau uguuggugca acacaaccag cacaugggug guuuauggga      360

ccugucauca caaaaaggga gaagccagac ggucccgcag agcugugaca cugccuucuc      420

auagcacccg gaaacuucag acgcgcuccc agaccuggcu ggagagcaga gaguacacaa      480

agcaccucau ccgggucgaa aauuggaucu uucgcaaccc cggcuucgcc cuggcggccg      540

cugccauugc uuggcugcuc gggucuucca ccagccagaa agugaucuau cugguuauga      600

uucuucugau cgccccugcg uacucuauua gaugcaucgg gguguccaau cgggacuuug      660

ucgagggaau gagcggcggg acaugggugg auauugugcu cgaacaugga ggcuguguca      720

ccguuauggc ccaagacaag cccacggugg auaucgagcu ggugacaacc acagucucua      780

acauggcuga ggugcgcucc uacugcuaug aagccagcau cuccgacaug gcuucugaua      840

gcagaugucc uacccagggg gaggccuacc uugacaagca guccgacacc caguauguuu      900

gcaaacggac acugguggau cgcggauggg gcaauggcug ugggcuguuc ggaaagggca      960

gccuggucac gugcgccaaa uuugcuugcu cuaagaaaau gaccgggaag uccauucaac     1020

cggaaaaccu ggaguacaga aucaugcuca gcgugcacgg aucucagcac agcggcauga     1080

uuguuaauga cacagggcau gaaaccgaug agaaccgggc gaagguggag aucacaccca     1140

auuccccuag agccgaagcu acccugggag gcuucggcuc ucuugggcug gacugugagc     1200

cccgcacggg acucgauuuu agcgaccugu auuaccuuac aaugaacaau aaacacuggc     1260

ugguccauaa ggaaugguuc cacgauauuc cucuccccug gcacgccggc gccgacaccg     1320

ggaccccgca uuggaacaau aaagaggcuc ugguggaauu uaaggaugcc cacgcgaaac     1380

ggcagacagu cgugguucug ggaucccagg agggcgcugu gcauaccgcc cucgccgggg     1440

cucuggaggc cgaaauggac ggcgcuaagg gaagacuuag cucuggccac cugaaaugcc     1500

gccucaagau ggauaagcug cggcuuaaag gggucuccua uagccugugu acagccgcgu     1560

ucacguuuac caagaucccu gccgagacac uccauggaac cgugacaguu gaagugcaau     1620

acgcuggcac cgacgggccc ugcaaagucc cugcccagau ggcuguggac augcagacgc     1680

ugacccccgu gggaagacug aucacagcca accccguuau uaccgagucu acagaaaacu     1740

ccaagaugau gcucgagcug gauccuccgu ucggcgacag cuacaucguc auuggggugg     1800

gcgagaaaaa gaucacccac cacuggcauc gcuccggauc uacgauuggc aaggcguucg     1860

aagccacagu gcggggcgcu aaaagaaugg ccguccuugg agauaccgcu ugggacuuug     1920

gcagcguugg gggagcccuc aauucccucg gcaaggggau ccaccaaauc uucggcgccg     1980

cuuuuaaauc ucuguucgga ggcaugagcu gguuuuccca gauucugauc gggacacuuc     2040

ucauguggcu gggacugaac accaagaaug gcagcauuuc ucucaugugu cuggcgcuug     2100

ggggagugcu gaucuuccuc uccaccgccg ugagcgcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10522
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10522
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggu gaccagacgg ggaagcgcuu acuauaugua ccuggaccgc aacgaugccg      180

gcgaagccau uuccuucccu acaacgcuug ggaugaauaa gugcuauauc caaauuaugg      240

accugggaca caccugugau gcuacaaugu cuuacgagug cccgaugcuc gacgaaggcg      300

uggagcccga ugacgucgau uguuggugca acaccacaag caccugggug guuuauggga      360

caugucauca caaaaaggga gaagccagac ggucccgcag agcugugacc cugccuucuc      420

auagcacgcg gaaacuucag acccgcuccc agacauggcu ggagagcaga gaguacacca      480

agcaccucau ccgggucgaa aauuggaucu uucgcaaccc cggcuucgcc cuggcggccg      540

cugccauugc uuggcugcuc gggucuucca caagccagaa agugaucuau cugguuauga      600

uucuucugau cgccccugcg uacucuauua gaugcaucgg gguguccaau cgggacuuug      660

ucgagggaau gagcggcggg accugggugg augugguccu cgaacaugga ggcuguguua      720

cggugauggc ccaagacaag cccacagugg auauugagcu ggucaccaca accgugucua      780

acauggcuga gguucgcucc uacugcuaug aagccagcau cuccgacaug gcuucugaua      840

gcagaugucc uacccagggg gaggccuacc uugacaagca guccgacaca caguaugugu      900

gcaaacggac gcuggucgau cgcggauggg gcaauggcug ugggcuguuc ggaaagggca      960

gccuggugac cugcgccaaa uuugcuugcu cuaagaaaau gacagggaag uccauccaac     1020

cggaaaaccu ggaguacaga auuaugcuca gcguucacgg aucucagcac agcggcauga     1080

ucgugaauga caccgggcau gaaacagaug agaaccgggc gaaggucgag auuaccccca     1140

auuccccuag agccgaagcu acgcugggag gcuucggcuc ucuugggcug gacugugagc     1200

cccgcacagg acucgauuuu agcgaccugu auuaccuuac caugaacaau aaacacuggc     1260

uggugcauaa ggaaugguuc cacgauaucc cucuccccug gcacgccggc gccgacaccg     1320

ggacaccgca uuggaacaau aaagaggcuc uggucgaauu uaaggaugcc cacgcgaaac     1380

ggcagaccgu gguugugcug ggaucccagg agggcgcugu ccauacagcc cucgccgggg     1440

cucuggaggc cgaaauggac ggcgcuaagg gaagacuuag cucuggccac cugaaaugcc     1500

gccucaagau ggauaagcug cggcuuaaag ggguguccua uagccugugu acggccgcgu     1560

ucaccuuuac aaagauuccu gccgagaccc uccauggaac aguuaccgug gaaguccaau     1620

acgcuggcac ggacgggccc ugcaaagugc cugcccagau ggcuguggac augcagaccc     1680

ugacacccgu uggaagacug aucaccgcca accccgucau cacagagucu accgaaaacu     1740

ccaagaugau gcucgagcug gauccuccgu ucggcgacag cuacauugug aucggggugg     1800

gcgagaaaaa gauuacgcac cacuggcauc gcuccggauc uacaaucggc aaggcguucg     1860

aagccaccgu ccggggcgcu aaaagaaugg ccgugcuugg agauacagcu ugggacuuug     1920

gcagcguugg gggagcccuc aauucccucg gcaaggggau ucaccaaauc uucggcgccg     1980

cuuuuaaauc ucuguucgga ggcaugagcu gguuuuccca gauccugauu gggacccuuc     2040

ucauguggcu gggacugaac gcgaagaaug gcagcaucuc ucucaugugu cuggcccuug     2100

ggggagugcu gauuuuccuc uccaccgcug ucagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10523
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10523
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcgau uaccagacgg ggaagcgcuu acuauaugua ccuggaccgc uccgaugccg      180

gcaaggccau cucuuucgcu acaacgcuug gggugaacaa augccacgug caaauuaugg      240

accugggaca uaugugugau gccaccauga gcuaugaaug cccuaugcuc gacgagggcg      300

ucgaaccgga ugacguggau uguuggugca auacaaccuc cacauggguu guguacggga      360

ccugucacca uaagaaagga gaggcuagac ggucucgcag agccgucaca cugcccagcc      420

acuccacccg gaagcugcag acgcgcagcc agaccuggcu ggaaucuaga gaguauacaa      480

aacaucucau caagguggag aacuggaucu uucggaaucc uggcuucgcg cugguugccg      540

uggcuauugc cuggcugcuc ggcuccagca ccucucagaa ggucaucuac cuggugauga      600

uucuucugau cgcucccgcc uauuccauuc gcugcaucgg ggugagcaac agagacuuug      660

ucgaaggaau gucuggcggg acauggguug auguggugcu cgagcacgga ggcuguguca      720

ccgugauggc gcaagacaaa ccuacgguug auauugaacu ggugacaacc acagucucca      780

auauggccga ggugcggagc uacugcuacg aggcuuccau cucugacaug gccagcgauu      840

cccgcugucc cacccagggg gaggcuuauc uugacaagca gagcgacacc caguacguuu      900

gcaaaagaac acugguggau cggggauggg gcaacggcug uggacucuuc gggaagggcu      960

cucuggucac gugcgccaaa uuuaccugcu ccaagaagau gacagggaaa agcauccaac     1020

cugaaaaucu ggaauaucgc auuaugcucu cugugcacgg aagccagcau uccggcauga     1080

ucggguacga gaccgacgaa gauagagcca aggucgaggu gacaccgaac ucuccccggg     1140

cugaggcgac ccugggaggc uucggcagcc uugggcugga cugugaaccu agaacgggac     1200

ucgauuuuuc cgaccuguau uaccuuacaa ugaauaacaa acacuggcug guucauaagg     1260

agugguucca cgauauuccc cucccuuggc acgccggcgc ugacaccggg acaccccauu     1320

ggaauaacaa agaagcccug guggaguuua aggaugccca cgcuaaacgc cagaccgucg     1380

ugguucuggg aagccaggaa ggcgccgugc auaccgcgcu cgcuggggcc cuggaggccg     1440

agauggacgg cgcuaaggga cggcuuuucu cuggccaccu gaagugcaga cucaaaaugg     1500

auaagcugcg ccuuaaaggg gucuccuaua gccuguguac agccgcuuuu acguucacca     1560

aggugccggc cgaaacacuc cauggaaccg ugacaguuga gguccaauac gcgggcaccg     1620

acgggccuug caaaaucccc gugcagaugg ccguggacau gcagacgcug accccugucg     1680

gacggcugau uacagcuaau cccgugauca ccgaaucuac agagaacucc aagaugaugc     1740

ucgaacugga ucccccuuuc ggcgacagcu acaucguuau uggggugggc gauaagaaaa     1800

ucacccacca cuggcauaga uccggaucua cgauuggcaa ggccuuugag gcuacagucc     1860

gcggggccaa acggauggcg gugcuuggag acaccgccug ggauuucggc agcguugggg     1920

gaguguuuaa uucccugggc aaggggaucc accaaauuuu cggcgcugcc uuuaaaucuc     1980

ucuucggagg caugagcugg uuuucccaga uccugaucgg gacacugcuu gucuggcucg     2040

gacugaacac caagaacggc agcauuucuc ugaccugucu cgcucuuggg ggagugauga     2100

ucuuccuuuc cacagccguu agcgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10524
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10524
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggu gaccagacgg ggaagcgcuu acuauaugua ccuggaccgc aacgaugccg      180

gcgaagccau uuccuucccu acaacgcuug ggaugaauaa gugcuauauc caaauuaugg      240

accugggaca caugugugau gcuaccaugu cuuacgagug cccgaugcuc gacgaaggcg      300

uggagcccga ugacgucgau uguuggugca acacaaccag cacaugggug guuuauggga      360

ccugucauca caaaaaggga gaagccagac ggucccgcag agcugugaca cugccuucuc      420

auagcacccg gaaacuucag acgcgcuccc agaccuggcu ggagagcaga gaguacacaa      480

agcaccucau ccgggucgaa aauuggaucu uucgcaaccc cggcuucgcc cuggcggccg      540

cugccauugc uuggcugcuc gggucuucca ccagccagaa agugaucuau cugguuauga      600

uucuucugau cgccccugcg uacucuauua gaugcaucgg gguguccaau cgggacuuug      660

ucgagggaau gagcggcggg acaugggugg augugguccu cgaacaugga ggcuguguua      720

ccgugauggc ccaagacaag cccacggugg auauugagcu ggucacaacc acagugucua      780

acauggcuga gguucgcucc uacugcuaug aagccagcau cuccgacaug gcuucugaua      840

gcagaugucc uacccagggg gaggccuacc uugacaagca guccgacacc caguaugugu      900

gcaaacggac acuggucgau cgcggauggg gcaauggcug ugggcuguuc ggaaagggca      960

gccuggugac gugcgccaaa uuugcuugcu cuaagaaaau gaccgggaag uccauccaac     1020

cggaaaaccu ggaguacaga auuaugcuca gcguucacgg aucucagcac agcggcauga     1080

ucgugaauga cacagggcau gaaaccgaug agaaccgggc gaaggucgag auuacaccca     1140

auuccccuag agccgaagcu acccugggag gcuucggcuc ucuugggcug gacugugagc     1200

cccgcacggg acucgauuuu agcgaccugu auuaccuuac aaugaacaau aaacacuggc     1260

uggugcauaa ggaaugguuc cacgauaucc cucuccccug gcacgccggc gccgacaccg     1320

ggaccccgca uuggaacaau aaagaggcuc uggucgaauu uaaggaugcc cacgcgaaac     1380

ggcagacagu gguugugcug ggaucccagg agggcgcugu ccauaccgcc cucgccgggg     1440

cucuggaggc cgaaauggac ggcgcuaagg gaagacuuag cucuggccac cugaaaugcc     1500

gccucaagau ggauaagcug cggcuuaaag ggguguccua uagccugugu acagccgcgu     1560

ucacguuuac caagauuccu gccgagacac uccauggaac cguuacagug gaaguccaau     1620

acgcuggcac cgacgggccc ugcaaagugc cugcccagau ggcuguggac augcagacgc     1680

ugacccccgu uggaagacug aucacagcca accccgucau caccgagucu acagaaaacu     1740

ccaagaugau gcucgagcug gauccuccgu ucggcgacag cuacauugug aucggggugg     1800

gcgagaaaaa gauuacccac cacuggcauc gcuccggauc uacgaucggc aaggcguucg     1860

aagccacagu ccggggcgcu aaaagaaugg ccgugcuugg agauaccgcu ugggacuuug     1920

gcagcguugg gggagcccuc aauucccucg gcaaggggau ucaccaaauc uucggcgccg     1980

cuuuuaaauc ucuguucgga ggcaugagcu gguuuuccca gauccugauu gggacacuuc     2040

ucauguggcu gggacugaac accaagaaug gcagcaucuc ucucaugugu cuggcgcuug     2100

ggggagugcu gauuuuccuc uccaccgccg ucagcgcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10525
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10525
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcguu acgcggcgcg      120

gcuccgcgua cuauauguac cuggauagaa acgacgcugg ggaggccauu ucuuuuccca      180

ccacacuugg aaugaauaag ugcuauaucc agauuaugga ucugggccau augugugacg      240

ccaccaugag cuacgaaugc ccuaugcucg augagggggu ggaaccggac gauguggacu      300

guuggugcaa cacaaccucc acaugggucg uguauggaac cugucaccau aaaaagggcg      360

aggcucggcg cucuagacgg gccguuacgc ugcccagcca cuccacccgc aaacuucaaa      420

caagaucuca gaccuggcug gaaagccggg aguacacaaa gcaucucauc cgcguggaga      480

auuggauuuu cagaaacccu ggguuugcuc uggccgcggc cgcuaucgcc uggcugcucg      540

gauccagcac cucucagaaa gucaucuauc uggugaugau ucuucugauc gcucccgccu      600

acuccauucg gugcaucggc guuagcaauc gcgauuucgu ggaaggcaug ucugggggaa      660

cgugggucga cauugugcuc gagcacggcg ggugugugac agucauggcg caggauaagc      720

cuaccguuga caucgaacug gugacaacca ccguguccaa cauggccgag gucagaagcu      780

acugcuauga ggcuucuauu uccgauaugg ccagcgacuc ccgguguccc acacaaggag      840

aggcuuaccu ugacaagcag ucugauacgc aguaugugug caaacgcacc cugguugaca      900

gaggcugggg gaauggaugu ggccucuuug gcaaggggag ccuggugaca ugcgccaaau      960

ucgccugcuc caagaaaaug accggaaaga gcauccagcc ugaaaaccug gaauaccgga     1020

ucaugcucuc uguccacggc ucccaacaua gcgggaugau ugugaaugau acaggacacg     1080

agaccgacga aaacagagcu aagguugaga ucacgcccaa uucuccccgc gcggaggcca     1140

cacugggcgg guuuggaagc cuuggccugg auugugaacc ucggaccggc cucgacuucu     1200

ccgaucugua uuaccuuacc augaacaaua aacauuggcu ggugcacaag gagugguuuc     1260

acgacauucc ccucccuugg caugcugggg ccgauacagg aaccccgcac uggaacaaua     1320

aagaagcccu ggucgaguuc aaggacgcuc augccaaaag acagacagug gucgugcugg     1380

gcucucagga aggggcgguu cacacggcuc ucgccggagc ccuggaggcu gagauggaug     1440

gcgccaaggg gcgccuuagc uccggccauc ugaaaugccg gcucaagaug gacaagcuga     1500

gacuuaaagg agugagcuau ucucugugua ccgcugccuu uacauucacc aagaucccgg     1560

cggaaacacu ccacggcacc gucacggugg agguucagua cgccgggacc gacggaccuu     1620

gcaaagugcc cgcucaaaug gccgucgaua ugcagacacu gaccccugug ggccgccuga     1680

uuacagcuaa ccccgugauc accgaaucca cggagaacag caagaugaug cucgaacugg     1740

aucccccuuu uggggacucu uacaucguua uuggagucgg cgagaaaaag aucacacacc     1800

auuggcaccg guccgggagc accauuggca aggccuucga ggcgacagug agaggagcca     1860

aacgcauggc ugugcuuggc gacaccgccu gggauuucgg guccgucgga ggcgcucuca     1920

auucucuggg gaagggaauc caucagauuu uuggcgccgc cuucaaaagc cuguuugggg     1980

gcauguccug guucucucaa auccugaucg gaacccuucu cauguggcug ggccugaaca     2040

caaagaaugg gagcauuucc cucauguguc uggcucuugg aggcgugcug aucuuucuca     2100

gcacggcggu uucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10526
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10526
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcguu acgcggcgcg      120

gcuccgcgua cuauauguac cuggauagaa acgacgcugg ggaggccauu ucuuuuccca      180

ccacacuugg aaugaauaag ugcuauaucc agauuaugga ucugggccau accugugacg      240

ccacaaugag cuacgaaugc ccuaugcucg augagggggu ggaaccggac gauguggacu      300

guuggugcaa caccacaucc accugggucg uguauggaac gugucaccau aaaaagggcg      360

aggcucggcg cucuagacgg gccguuaccc ugcccagcca cuccacacgc aaacuucaaa      420

ccagaucuca gacauggcug gaaagccggg aguacaccaa gcaucucauc cgcguggaga      480

auuggauuuu cagaaacccu ggguuugcuc uggccgcggc cgcuaucgcc uggcugcucg      540

gauccagcac gucucagaaa gucaucuauc uggugaugau ucuucugauc gcucccgccu      600

acuccauucg gugcaucggc guuagcaauc gcgauuucgu ggaaggcaug ucugggggaa      660

caugggucga cguggugcuc gagcacggcg ggugugucac cguuauggcg caggauaagc      720

cuacagugga cauugaacug gugaccacca cagucuccaa cauggccgag gugagaagcu      780

acugcuauga ggcuucuauc uccgauaugg ccagcgacuc ccgguguccc acgcaaggag      840

aggcuuaccu ugacaagcag ucugauaccc aguauguuug caaacgcaca cugguggaca      900

gaggcugggg gaauggaugu ggccucuuug gcaaggggag ccuggugacc ugcgccaaau      960

ucgccugcuc caagaaaaug acaggaaaga gcauucagcc ugaaaaccug gaauaccgga     1020

ucaugcucuc uguccacggc ucccaacaua gcgggaugau cguuaaugau accggacacg     1080

agacggacga aaacagagcu aagguggaga uuacacccaa uucuccccgc gcggaggcca     1140

cccugggcgg guuuggaagc cuuggccugg auugugaacc ucggaccggc cucgacuucu     1200

ccgaucugua uuaccuuaca augaacaaua aacauuggcu gguccacaag gagugguuuc     1260

acgacauccc ccucccuugg caugcugggg ccgauaccgg aacaccccac uggaacaaua     1320

aagaagcccu gguggaguuc aaggacgcuc augccaaaag acagacgguc gugguucugg     1380

gcucucagga aggggcggug cacaccgcuc ucgccggagc ccuggaggcu gagauggaug     1440

gcgccaaggg gcgccuuagc uccggccauc ugaaaugccg gcucaagaug gacaagcuga     1500

gacuuaaagg agucagcuau ucucugugua cagcugccuu uaccuucaca aagauuccgg     1560

cggaaacccu ccacggcacg gugaccguug aggugcagua cgccgggaca gacggaccuu     1620

gcaaaguccc cgcucaaaug gccguggaua ugcagacccu gacaccugug ggccgccuga     1680

ucaccgcuaa ccccguuauu acggaaucca cagagaacag caagaugaug cucgaacugg     1740

acccgccuuu uggggauucu uacaucguca ucggaguggg cgagaaaaag auuacccacc     1800

auuggcaccg guccgggagc acaaucggca aggccuucga ggcgaccgug agaggagcca     1860

aacgcauggc uguccuuggc gacaccgccu gggauuucgg guccguggga ggcgcucuca     1920

auucucuggg gaagggcauu caucagaucu uuggagccgc cuucaaaagc cuguuugggg     1980

gcauguccug guucucucaa auucugaucg gaacacuucu cauguggcug ggccugaacg     2040

cuaagaaugg gagcaucucc cucauguguc uggcgcuugg aggcguucug auuuuucuca     2100

gcacggccgu gucugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10527
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10527
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcauu acgcggcgcg      120

gcuccgcgua cuauauguac cuggauagau cugacgcugg gaaggccauc agcuuugcca      180

ccacacuugg aguuaacaaa ugccaugugc agauuaugga ucugggccac augugugacg      240

cuaccauguc cuaugagugc cccaugcucg augaaggggu ggagcccgac gaugucgacu      300

guuggugcaa uacaaccucu acaugggugg uuuacggaac cugucaucac aagaaaggcg      360

aagcccggcg cagcagacgg gcugugacgc ugccguccca uucuacccgc aaacuucaaa      420

caagaagcca gaccuggcug gagucccggg aauauacaaa gcaccucauc aaggucgaga      480

acuggauuuu ccgcaauccc ggguuugccc ugguggcggu ugccaucgcu uggcugcucg      540

gaagcucuac cucccagaag gugaucuacc uggucaugau ucuucugauc gccccugcuu      600

auagcauuag augcaucggc gugucuaacc gggauuucgu ggagggcaug uccgggggaa      660

cgugggucga cguugugcuc gaacacggcg ggugugugac agucauggcc caggauaaac      720

ccaccgugga cauugagcug guuacaacca ccgugagcaa uauggcggaa guccgcucuu      780

acugcuacga ggccuccauc agcgauaugg cuuccgacuc uagauguccu acacaaggag      840

aggccuaucu ugacaagcag agcgauacgc aguacgugug caaacggacc cugguugacc      900

gcggcugggg gaacggaugu ggccucuuug gcaagggguc ccuggugaca ugcgcuaaau      960

uuaccugcag caagaagaug acaggaaaau cuauucagcc cgaaaaucug gaguauagaa     1020

ucaugcucuc cguccauggc agccaacacu cugggaugau cggauacgaa accgaugagg     1080

accgggccaa gguggaaguc acgccuaaca gcccgagagc cgaggcuaca cugggcgggu     1140

uuggaucccu uggccuggau ugugagcccc gcaccggccu cgacuucucu gaucuguauu     1200

accuuaccau gaauaacaaa cauuggcugg ugcacaagga augguuucac gacauuccuc     1260

uccccuggca ugcgggggcc gauacaggaa ccccucacug gaauaacaaa gaggcucugg     1320

uugaguucaa ggacgcccau gccaaacggc agacaguggu cgugcugggc agccaggaag     1380

gggcuguuca cacggcccuc gcgggagcuc uggaagccga gauggauggc gccaagggga     1440

gacuuuucuc cggccaucug aagugccgcc ucaaaaugga caagcugcgg cuuaaaggag     1500

ugagcuauuc ucuguguacc gcugccuuca cauuuaccaa gguccccgcu gagacacucc     1560

acggcaccgu gacgguggaa guucaguacg ccgggaccga cggaccgugc aaaaucccug     1620

uccaaauggc gguggauaug cagacacuga cccccguggg cagacugauu acagccaauc     1680

cugucaucac cgaguccacg gaaaacagca agaugaugcu cgagcuggac ccucccuucg     1740

gggauucuua cauugugauc ggaguuggcg acaagaaaau cacacaccau uggcaccgcu     1800

ccgggagcac cauuggcaag gcuuucgaag ccacagugcg gggagcuaaa agaauggccg     1860

uccuuggcga uaccgcgugg gacuuugggu ccgugggagg cguuuucaau ucucugggga     1920

agggaaucca ucagauuuuu ggcgccgcuu ucaaaagccu cuuugggggc auguccuggu     1980

ucucucaaau ccugauugga acccugcuug uguggcucgg ccugaacaca aagaacggga     2040

gcaucucccu gacgugucuc gcccugggag gcgucaugau cuuucuuagc accgcugugu     2100

cugccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10528
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10528
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcguu acgcggcgcg      120

gcuccgcgua cuauauguac cuggauagaa acgacgcugg ggaggccauu ucuuuuccca      180

ccacacuugg aaugaauaag ugcuauaucc agauuaugga ucugggccau augugugacg      240

ccaccaugag cuacgaaugc ccuaugcucg augagggggu ggaaccggac gauguggacu      300

guuggugcaa cacaaccucc acaugggucg uguauggaac cugucaccau aaaaagggcg      360

aggcucggcg cucuagacgg gccguuacgc ugcccagcca cuccacccgc aaacuucaaa      420

caagaucuca gaccuggcug gaaagccggg aguacacaaa gcaucucauc cgcguggaga      480

auuggauuuu cagaaacccu ggguuugcuc uggccgcggc cgcuaucgcc uggcugcucg      540

gauccagcac cucucagaaa gucaucuauc uggugaugau ucuucugauc gcucccgccu      600

acuccauucg gugcaucggc guuagcaauc gcgauuucgu ggaaggcaug ucugggggaa      660

cgugggucga cguggugcuc gagcacggcg ggugugucac aguuauggcg caggauaagc      720

cuaccgugga cauugaacug gugacaacca ccgucuccaa cauggccgag gugagaagcu      780

acugcuauga ggcuucuauc uccgauaugg ccagcgacuc ccgguguccc acacaaggag      840

aggcuuaccu ugacaagcag ucugauacgc aguauguuug caaacgcacc cugguggaca      900

gaggcugggg gaauggaugu ggccucuuug gcaaggggag ccuggucaca ugcgccaaau      960

ucgccugcuc caagaaaaug accggaaaga gcauucagcc ugaaaaccug gaauaccgga     1020

ucaugcucuc ugugcacggc ucccaacaua gcgggaugau cguuaaugau acaggacacg     1080

agaccgacga aaacagagcu aagguggaga uuacgcccaa uucuccccgc gcggaggcca     1140

cacugggcgg guuuggaagc cuuggccugg auugugaacc ucggaccggc cucgacuucu     1200

ccgaucugua uuaccuuacc augaacaaua aacauuggcu gguccacaag gagugguuuc     1260

acgacauccc ccucccuugg caugcugggg ccgauacagg aaccccgcac uggaacaaua     1320

aagaagcccu gguggaguuc aaggacgcuc augccaaaag acagacaguc gugguucugg     1380

gcucucagga aggggcggug cacacggcuc ucgccggagc ccuggaggcu gagauggaug     1440

gcgccaaggg gcgccuuagc uccggccauc ugaaaugccg gcucaagaug gacaagcuga     1500

gacuuaaagg agucagcuau ucucugugua ccgcugccuu uacauucacc aagauuccgg     1560

cggaaacacu ccacggcacc gugacgguug aggugcagua cgccgggacc gacggaccuu     1620

gcaaaguccc cgcucaaaug gccguggaua ugcagacacu gaccccugug ggccgccuga     1680

ucacagcuaa ccccguuauu accgaaucca cggagaacag caagaugaug cucgaacugg     1740

aucccccuuu uggggacucu uacaucguca ucggaguggg cgagaaaaag auuacacacc     1800

auuggcaccg guccgggagc accaucggca aggccuucga ggcgacagug agaggagcca     1860

aacgcauggc uguccuuggc gacaccgccu gggauuucgg guccguggga ggcgcucuca     1920

auucucuggg gaagggcauu caucagaucu uuggagccgc cuucaaaagc cuguuugggg     1980

gcauguccug guucucucaa auucugaucg gaacccuucu cauguggcug ggccugaaca     2040

caaagaaugg gagcaucucc cucauguguc uggcucuugg aggcguucug auuuuucuca     2100

gcacggcggu gucugccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10529
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10529
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccguuac ccggcgcggc agcgccuacu auauguaccu ggacagaaac gaugcugggg      180

aggccaucuc cuuccccaca acgcuuggaa ugaauaaaug uuauauucag aucauggacc      240

ugggccacau gugcgaugcu accaugucuu acgaaugucc uaugcucgac gagggggugg      300

aaccggauga cguggauugc ugguguaaca caaccagcac augggucgug uauggaaccu      360

gccaucacaa gaaaggcgag gcccggcgcu ccagacgggc gguuacacug cccucucaua      420

gcacccgcaa acuucaaacg agaucccaga ccuggcugga aagccgggag uacacaaaac      480

accucauucg cguggagaau uggaucuuua gaaacccugg cuucgcccug gcugccgcug      540

ccaucgcgug gcugcucggg ucuuccacca gccagaaggu cauuuaucug gugaugaucc      600

uucugauugc ccccgcuuac ucuauccggu guauuggagu uuccaaucgc gacuuugugg      660

aaggcaugag cgggggaaca ugggucgaua ucgugcucga gcauggcggg ugcgugaccg      720

ucauggccca ggacaagccu acgguugaua uugaacuggu gacaaccaca gugucuaaca      780

uggcugaggu cagauccuac uguuaugagg ccagcaucuc cgacauggcc ucugauagcc      840

ggugccccac ccaaggagag gcuuaccuug acaaacaguc cgacacccag uaugugugua      900

agcgcacacu gguugauaga ggcuggggca augggugcgg acucuucggc aaagggagcc      960

uggugacgug cgcgaaguuu gccuguucua aaaagaugac cggaaagucc auccagccug     1020

aaaaccugga auaccggauu augcucagcg uccacggcuc ucaacacagc gggaugaucg     1080

ugaaugacac aggacaugag accgaugaaa acagagcuaa aguugagauu acacccaauu     1140

ccccgcgcgc cgaggccacc cugggcggcu ucgggucucu uggacuggac ugcgaaccuc     1200

ggacgggccu cgauuuuagc gaccuguauu accuuacaau gaacaauaag cacuggcugg     1260

ugcauaaaga gugguuccac gauauccccc ucccuuggca cgcuggggcc gacaccggaa     1320

caccccauug gaacaauaag gaagcgcugg ucgaguuuaa agaugcucac gccaagagac     1380

agaccguggu cgugcugggc ucccaggaag gggccguuca uaccgcucuc gccggcgcuc     1440

uggaggccga gauggacgga gcgaaaggcc gccuuagcuc ugggcaccug aagugucggc     1500

ucaagaugga uaaacugaga cuuaagggag uguccuauag ccugugcaca gccgcuuuca     1560

cguuuaccaa aauuccggcc gaaacacucc auggcaccgu cacaguggag guucaguacg     1620

cugggaccga cggaccuugu aaggugcccg cccaaauggc ggucgacaug cagacgcuga     1680

ccccuguggg ccgccugauc acagccaacc ccgugaucac cgaaucuaca gagaacucca     1740

aaaugaugcu cgaacuggau cccccuuucg gggacagcua cauuguuauc ggcgucggag     1800

agaagaagau uacccaccac uggcaucggu ccggcucuac gaucgggaag gcuuuugagg     1860

ccacagugag aggagcuaag cgcauggccg ugcuuggcga uaccgccugg gacuucggga     1920

gcgucggagg cgcucucaau ucccugggga aaggcauuca ccagaucuuc ggagcggccu     1980

uuaagucucu guucggcggg augagcuggu uuucccaaau ccugauugga acacuucuca     2040

uguggcuggg ccugaacacc aaaaauggga gcaucucucu caugugccug gcucuuggag     2100

gcgugcugau uuuccucucc accgccguua gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10530
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10530
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccguuac ccggcgcggc agcgccuacu auauguaccu ggacagaaac gaugcugggg      180

aggccaucuc cuuccccaca acgcuuggaa ugaauaaaug uuauauucag aucauggacc      240

ugggccauac cugcgaugcu acaaugucuu acgaaugucc uaugcucgac gagggggugg      300

aaccggauga cguggauugc ugguguaaca ccacaagcac cugggucgug uauggaacau      360

gccaccacaa gaaaggcgag gcccggcgcu ccagacgggc gguuacccug cccucucaua      420

gcacgcgcaa acuucaaacc agaucccaga cauggcugga aagccgggag uacaccaaac      480

accucauucg cguggagaau uggaucuuua gaaacccugg cuucgcccug gcugccgcug      540

ccaucgcgug gcugcucggg ucuuccacaa gccagaaggu cauuuaucug gugaugaucc      600

uucugauugc ccccgcuuac ucuauccggu guauuggagu uuccaaucgc gacuuugugg      660

aaggcaugag cgggggaacc ugggucgaug uggugcucga gcauggcggg ugcgucacgg      720

uuauggccca ggacaagccu acaguggaua ucgaacuggu gaccacaacc gucucuaaca      780

uggcugaggu gagauccuac uguuaugagg ccagcauuuc cgacauggcc ucugauagcc      840

ggugccccac ccaaggagag gcuuaccuug acaaacaguc cgacacacag uauguuugua      900

agcgcacgcu gguggauaga ggcuggggca augggugcgg acucuucggc aaagggagcc      960

uggugaccug cgcgaaguuu gccuguucua aaaagaugac aggaaagucc auccagccug     1020

aaaaccugga auaccggauc augcucagcg uccacggcuc ucaacacagc gggaugauug     1080

uuaaugacac cggacaugag acagaugaaa acagagcuaa aguggagauc acccccaauu     1140

ccccgcgcgc cgaggccacg cugggcggcu ucgggucucu uggacuggac ugcgaaccuc     1200

ggacaggccu cgauuuuagc gaccuguauu accuuaccau gaacaauaag cacuggcugg     1260

uccauaaaga gugguuccac gauauucccc ucccuuggca cgcuggggcc gacaccggaa     1320

caccccauug gaacaauaag gaagcgcugg uggaguuuaa agaugcucac gccaagagac     1380

agaccgucgu gguucugggc ucccaggaag gggccgugca uacagcucuc gccggcgcuc     1440

uggaggccga gauggacgga gcgaaaggcc gccuuagcuc ugggcaccug aagugucggc     1500

ucaagaugga uaaacugaga cuuaagggag ucuccuauag ccugugcacg gccgcuuuca     1560

ccuuuacaaa aaucccggcc gaaacccucc auggcacagu gaccguugag gugcaguacg     1620

cugggacgga cggaccuugu aagguccccg cccaaauggc gguggacaug cagacccuga     1680

caccuguggg ccgccugauu accgccaacc ccguuaucac agaaucuacc gagaacucca     1740

aaaugaugcu cgaacuggau cccccuuucg gggacagcua caucgucauu ggcgugggag     1800

agaagaagau cacgcaccac uggcaucggu ccggcucuac aauugggaag gcuuuugagg     1860

ccaccgugag aggagcuaag cgcauggccg uccuuggcga uacagccugg gacuucggga     1920

gcgugggagg cgcucucaau ucccugggga aaggcaucca ccagauuuuc ggagcggccu     1980

uuaagucucu guucggcggg augagcuggu uuucccaaau ccugaucgga acccuucuca     2040

uguggcuggg ccugaacgcu aaaaauggga gcauuucucu caugugccug gcccuuggag     2100

gcguucugau cuuccucucc accgccguga gcgcuugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10531
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10531
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccaucac ccggcgcggc agcgccuacu auauguaccu ggacagaucc gaugcuggga      180

aagccauuuc uuucgcuaca acgcuuggag uuaacaagug ucacgugcag aucauggacc      240

ugggccauau gugcgaugcc accaugagcu augagugucc caugcucgac gaaggggugg      300

agcccgauga cgucgauugc ugguguaaua caaccuccac augggugguu uacggaaccu      360

gccaccauaa aaagggcgaa gcgcggcgcu cuagacgggc cgugacacug ccgagccacu      420

ccacccgcaa acuucaaacg agaagccaga ccuggcugga gucucgggaa uauacaaagc      480

aucucauuaa ggucgagaac uggaucuuuc gcaaucccgg cuucgcucug guggccguug      540

cuaucgccug gcugcucggg uccagcaccu cucagaaagu gauuuaccug gucaugaucc      600

uucugauugc gccugccuau uccaucagau guauuggagu gagcaaccgg gacuuugugg      660

agggcauguc ugggggaaca ugggucgaug uugugcucga acacggcggg ugcgugaccg      720

ucauggcuca ggacaagccc acgguggaua ucgagcuggu uacaaccaca guguccaaua      780

uggccgaagu ccgcagcuac uguuacgagg cuuccauuuc ugacauggcc agcgauucca      840

gaugcccuac ccaaggagag gccuaucuug acaaacagag cgacacccag uacgugugua      900

agcggacacu gguugaucgc ggcuggggca acgggugcgg acucuucggc aaagggucuc      960

uggugacgug cgcuaaguuu accuguucca agaaaaugac aggaaagagc auccagcccg     1020

aaaaucugga guauagaauc augcucucug uccacggcag ccaacauucc gggaugauug     1080

gauacgaaac cgacgaggau cgggcgaaag uggaagucac accuaacucu ccgagagccg     1140

aggcuacccu gggcggcuuc gggagccuug gacuggacug cgagccccgc acgggccucg     1200

auuuuuccga ccuguauuac cuuacaauga auaacaagca cuggcuggug cauaaagaau     1260

gguuccacga uaucccucuc cccuggcacg ccggggccga caccggaacc ccucauugga     1320

auaacaagga ggcucugguu gaauuuaaag augcccacgc gaagcggcag acaguggucg     1380

ugcugggcag ccaggagggg gcuguucaua ccgcccucgc cggcgcucug gaagccgaga     1440

uggacggagc uaagggcaga cuuuucucug ggcaccugaa augucgccuc aagauggaua     1500

aacugcggcu uaagggagug uccuauagcc ugugcacagc cgcguuuacg uucaccaaag     1560

uccccgccga gacacuccau ggcaccguga caguggaagu ucaguacgcu gggaccgacg     1620

gaccguguaa gauuccuguc caaauggccg uggacaugca gacgcugacc cccgugggca     1680

gacugaucac agcuaauccu gucauuaccg agucuacaga aaacuccaag augaugcucg     1740

agcuggaucc ucccuuuggg gacagcuaca ucgugaucgg cguuggagau aaaaagauua     1800

cccaccacug gcaucgcucc ggcucuacga ucgggaaagc cuucgaagcg acagugcggg     1860

gagccaagag aauggcuguc cuuggcgaca ccgccuggga uuucgggagc gugggaggcg     1920

uuuuuaauuc ccuggggaaa ggcauucacc agaucuucgg agcugccuuu aagucucucu     1980

ucggcgggau gagcugguuu ucccaaauuc ugaucggaac acugcuugug uggcucggcc     2040

ugaacaccaa gaacgggagc aucucucuga ccugccucgc ccugggaggc gucaugauuu     2100

uccuuuccac agcugugagc gcgugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10532
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10532
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccguuac ccggcgcggc agcgccuacu auauguaccu ggacagaaac gaugcugggg      180

aggccaucuc cuuccccaca acgcuuggaa ugaauaaaug uuauauucag aucauggacc      240

ugggccacau gugcgaugcu accaugucuu acgaaugucc uaugcucgac gagggggugg      300

aaccggauga cguggauugc ugguguaaca caaccagcac augggucgug uauggaaccu      360

gccaucacaa gaaaggcgag gcccggcgcu ccagacgggc gguuacacug cccucucaua      420

gcacccgcaa acuucaaacg agaucccaga ccuggcugga aagccgggag uacacaaaac      480

accucauucg cguggagaau uggaucuuua gaaacccugg cuucgcccug gcugccgcug      540

ccaucgcgug gcugcucggg ucuuccacca gccagaaggu cauuuaucug gugaugaucc      600

uucugauugc ccccgcuuac ucuauccggu guauuggagu uuccaaucgc gacuuugugg      660

aaggcaugag cgggggaaca ugggucgaug uggugcucga gcauggcggg ugcgucaccg      720

uuauggccca ggacaagccu acgguggaua ucgaacuggu gacaaccaca gucucuaaca      780

uggcugaggu gagauccuac uguuaugagg ccagcauuuc cgacauggcc ucugauagcc      840

ggugccccac ccaaggagag gcuuaccuug acaaacaguc cgacacccag uauguuugua      900

agcgcacacu gguggauaga ggcuggggca augggugcgg acucuucggc aaagggagcc      960

uggucacgug cgcgaaguuu gccuguucua aaaagaugac cggaaagucc auccagccug     1020

aaaaccugga auaccggauc augcucagcg ugcacggcuc ucaacacagc gggaugauug     1080

uuaaugacac aggacaugag accgaugaaa acagagcuaa aguggagauc acacccaauu     1140

ccccgcgcgc cgaggccacc cugggcggcu ucgggucucu uggacuggac ugcgaaccuc     1200

ggacgggccu cgauuuuagc gaccuguauu accuuacaau gaacaauaag cacuggcugg     1260

uccauaaaga gugguuccac gauauucccc ucccuuggca cgcuggggcc gacaccggaa     1320

caccccauug gaacaauaag gaagcgcugg uggaguuuaa agaugcucac gccaagagac     1380

agaccgucgu gguucugggc ucccaggaag gggccgugca uaccgcucuc gccggcgcuc     1440

uggaggccga gauggacgga gcgaaaggcc gccuuagcuc ugggcaccug aagugucggc     1500

ucaagaugga uaaacugaga cuuaagggag ucuccuauag ccugugcaca gccgcuuuca     1560

cguuuaccaa aaucccggcc gaaacacucc auggcaccgu gacaguugag gugcaguacg     1620

cugggaccga cggaccuugu aagguccccg cccaaauggc gguggacaug cagacgcuga     1680

ccccuguggg ccgccugauu acagccaacc ccguuaucac cgaaucuaca gagaacucca     1740

aaaugaugcu cgaacuggau cccccuuucg gggacagcua caucgucauu ggcgugggag     1800

agaagaagau cacccaccac uggcaucggu ccggcucuac gauugggaag gcuuuugagg     1860

ccacagugag aggagcuaag cgcauggccg uccuuggcga uaccgccugg gacuucggga     1920

gcgugggagg cgcucucaau ucccugggga aaggcaucca ccagauuuuc ggagcggccu     1980

uuaagucucu guucggcggg augagcuggu uuucccaaau ccugaucgga acacuucuca     2040

uguggcuggg ccugaacacc aaaaauggga gcauuucucu caugugccug gcucuuggag     2100

gcguucugau cuuccucucc accgccguga gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10533
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10533
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggu gaccagacgg ggaagcgcuu acuauaugua ccuggaccgc aacgaugccg      180

gcgaagccau uuccuucccu acaacgcuug ggaugaauaa gugcuauauc caaauuaugg      240

accugggaca caugugugau gcuaccaugu cuuacgagug cccgaugcuc gacgaaggcg      300

uggagcccga ugacgucgau uguuggugca acacaaccag cacaugggug guuuauggga      360

ccugucauca caaaaaggga gaagccagac ggucccgcag agcugugaca cugccuucuc      420

auagcacccg gaaacuucag acgcgcuccc agaccuggcu ggagagcaga gaguacacaa      480

agcaccucau ccgggucgaa aauuggaucu uucgcaaccc cggcuucgcc cuggcggccg      540

cugccauugc uuggcugcuc gggucuucca ccagccagaa agugaucuau cugguuauga      600

uucuucugau cgccccugcg uacucuauua gaugcaucgg gguguccaau cgggacuuug      660

ucgagggaau gagcggcggg acaugggugg auauugugcu cgaacaugga ggcuguguca      720

ccguuauggc ccaagacaag cccacggugg auaucgagcu ggugacaacc acagucucua      780

acauggcuga ggugcgcucc uacugcuaug aagccagcau cuccgacaug gcuucugaua      840

gcagaugucc uacccagggg gaggccuacc uugacaagca guccgacacc caguauguuu      900

gcaaacggac acugguggau cgcggauggg gcaauggcug uggccucuuc ggaaagggca      960

gccuggucac gugcgccaaa uuugcuugcu cuaagaaaau gaccgggaag uccauucaac     1020

cggaaaaccu ggaguacaga aucaugcuca gcgugcacgg aucucagcac agcggcauga     1080

uuguuaauga cacagggcau gaaaccgaug agaaccgggc gaagguggag aucacaccca     1140

auuccccuag agccgaagcu acccugggag gcuucggcuc ucuugggcug gacugugagc     1200

cccgcacggg acucgauuuu agcgaccugu auuaccuuac aaugaacaau aaacacuggc     1260

ugguccauaa ggaaugguuc cacgauauuc cucuccccug gcacgccggc gccgacaccg     1320

ggaccccgca uuggaacaau aaagaggcuc ugguggaauu uaaggaugcc cacgcgaaac     1380

ggcagacagu cgugguucug ggaucccagg agggcgcugu gcauaccgcc cucgccgggg     1440

cucuggaggc cgaaauggac ggcgcuaagg gaagacuuag cucuggccac cugaaaugcc     1500

gccucaagau ggauaagcug cggcuuaaag gggucuccua uagccugugu acagccgcgu     1560

ucacguuuac caagaucccu gccgagacac uccauggaac cgugacaguu gaagugcaau     1620

acgcuggcac cgacgggccc ugcaaagucc cugcccagau ggcuguggac augcagacgc     1680

ugacccccgu gggaagacug aucacagcca accccguuau uaccgagucu acagaaaacu     1740

ccaagaugau gcucgagcug gauccuccgu ucggcgacag cuacaucguc auuggggugg     1800

gcgagaaaaa gaucacccac cacuggcauc gcuccggauc uacgcuuggc aaggcguuca     1860

gcacaacccu gaaaggggcc caacggcucg cugcccuggg agauacagcu ugggacuuug     1920

gcuccauugg gggaguguuc aauucuaucg gcagagccgu ccaccaggug uuugggggcg     1980

ccuuccgcac ccuguuugga ggcaugagcu ggaucaccca ggggcuuaug ggagcucucc     2040

ugcuguggau gggcguuaac gcgcgggaua gauccauugc ccucgcuuuc cuggccacag     2100

ggggagugcu ugucuuucug gccacgaaug ugcaugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10534
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10534
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggu gaccagacgg ggaagcgcuu acuauaugua ccuggaccgc aacgaugccg      180

gcgaagccau uuccuucccu acaacgcuug ggaugaauaa gugcuauauc caaauuaugg      240

accugggaca caccugugau gcuacaaugu cuuacgagug cccgaugcuc gacgaaggcg      300

uggagcccga ugacgucgau uguuggugca acaccacaag caccugggug guuuauggga      360

caugucauca caaaaaggga gaagccagac ggucccgcag agcugugacc cugccuucuc      420

auagcacgcg gaaacuucag acccgcuccc agacauggcu ggagagcaga gaguacacca      480

agcaccucau ccgggucgaa aauuggaucu uucgcaaccc cggcuucgcc cuggcggccg      540

cugccauugc uuggcugcuc gggucuucca caagccagaa agugaucuau cugguuauga      600

uucuucugau cgccccugcg uacucuauua gaugcaucgg gguguccaau cgggacuuug      660

ucgagggaau gagcggcggg accugggugg augugguccu cgaacaugga ggcuguguua      720

cggugauggc ccaagacaag cccacagugg auauugagcu ggucaccaca accgugucua      780

acauggcuga gguucgcucc uacugcuaug aagccagcau cuccgacaug gcuucugaua      840

gcagaugucc uacccagggg gaggccuacc uugacaagca guccgacaca caguaugugu      900

gcaaacggac gcuggucgau cgcggauggg gcaauggcug uggccucuuc ggaaagggca      960

gccuggugac cugcgccaaa uuugcuugcu cuaagaaaau gacagggaag uccauccaac     1020

cggaaaaccu ggaguacaga auuaugcuca gcguucacgg aucucagcac agcggcauga     1080

ucgugaauga caccgggcau gaaacagaug agaaccgggc gaaggucgag auuaccccca     1140

auuccccuag agccgaagcu acgcugggag gcuucggcuc ucuugggcug gacugugagc     1200

cccgcacagg acucgauuuu agcgaccugu auuaccuuac caugaacaau aaacacuggc     1260

uggugcauaa ggaaugguuc cacgauaucc cucuccccug gcacgccggc gccgacaccg     1320

ggacaccgca uuggaacaau aaagaggcuc uggucgaauu uaaggaugcc cacgcgaaac     1380

ggcagaccgu gguugugcug ggaucccagg agggcgcugu ccauacagcc cucgccgggg     1440

cucuggaggc cgaaauggac ggcgcuaagg gaagacuuag cucuggccac cugaaaugcc     1500

gccucaagau ggauaagcug cggcuuaaag ggguguccua uagccugugu acggccgcgu     1560

ucaccuuuac aaagauuccu gccgagaccc uccauggaac aguuaccgug gaaguccaau     1620

acgcuggcac ggacgggccc ugcaaagugc cugcccagau ggcuguggac augcagaccc     1680

ugacacccgu uggaagacug aucaccgcca accccgucau cacagagucu accgaaaacu     1740

ccaagaugau gcucgagcug gauccuccgu ucggcgacag cuacauugug aucggggugg     1800

gcgagaaaaa gauuacgcac cacuggcauc gcuccggauc uacacuuggc aaggcguuca     1860

gcaccacacu gaaaggggcc caacggcucg cugcccuggg agauaccgcu ugggacuuug     1920

gcuccaucgg gggagucuuc aauucuauug gcagagccgu gcaccagguu uuugggggcg     1980

ccuuccgcac ccuguuugga ggcaugagcu ggaucacaca ggggcuuaug ggagcucucc     2040

ugcuguggau gggcgugaac gcgcgggaua gauccaucgc ccucgcuuuc cuggccaccg     2100

ggggaguccu uguguuucug gccacgaaug uucaugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10535
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10535
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcgau uaccagacgg ggaagcgcuu acuauaugua ccuggaccgc uccgaugccg      180

gcaaggccau cucuuucgcu acaacgcuug gggugaacaa augccacgug caaauuaugg      240

accugggaca uaugugugau gccaccauga gcuaugaaug cccuaugcuc gacgagggcg      300

ucgaaccgga ugacguggau uguuggugca auacaaccuc cacauggguu guguacggga      360

ccugucacca uaagaaagga gaggcuagac ggucucgcag agccgucaca cugcccagcc      420

acuccacccg gaagcuccag acgcgcagcc agaccuggcu ggaaucuaga gaguauacaa      480

aacaucucau caagguggag aacuggaucu uucggaaucc uggcuucgcg cugguugccg      540

uggcuauugc cuggcugcuc ggcuccagca ccucucagaa ggucaucuac cuggugauga      600

uucuucugau cgcucccgcc uauuccauuc gcugcaucgg ggugagcaac agagacuuug      660

ucgaaggaau gucuggcggg acauggguug auguggugcu cgagcacgga ggcuguguca      720

ccgugauggc gcaagacaaa ccuacgguug auauugaacu ggugacaacc acagucucca      780

auauggccga ggugcggagc uacugcuacg aggcuuccau cucugacaug gccagcgauu      840

cccgcugucc cacccagggg gaggcuuauc uugacaagca gagcgacacc caguacguuu      900

gcaaaagaac acugguggau cggggauggg gcaacggcug ugggcuuuuc ggaaagggcu      960

cucuggucac gugcgccaaa uuuaccugcu ccaagaagau gacagggaaa agcauccaac     1020

cugaaaaucu ggaauaucgc auuaugcucu cugugcacgg aagccagcau uccggcauga     1080

ucggguacga gaccgacgaa gauagagcca aggucgaggu gacaccgaac ucuccccggg     1140

cugaggcgac ccugggaggc uucggcagcc uugggcugga cugugaaccu agaacgggac     1200

ucgauuuuuc cgaccuguau uaccuuacaa ugaauaacaa acacuggcug guucauaagg     1260

agugguucca cgauauuccc cucccuuggc acgccggcgc ugacaccggg acaccccauu     1320

ggaauaacaa agaagcccug guggaguuua aggaugccca cgcuaaacgc cagaccgucg     1380

ugguucuggg aagccaggaa ggcgccgugc auaccgcgcu cgcuggggcc cuggaggccg     1440

agauggacgg cgcuaaggga cggcuuuucu cuggccaccu gaagugcaga cucaaaaugg     1500

auaagcugcg ccuuaaaggg gucuccuaua gccuguguac agccgcuuuu acguucacca     1560

aggugccggc cgaaacacuc cauggaaccg ugacaguuga gguccaauac gcgggcaccg     1620

acgggccuug caaaaucccc gugcagaugg ccguggacau gcagacgcug accccugucg     1680

gacggcugau uacagcuaau cccgugauca ccgaaucuac agagaacucc aagaugaugc     1740

ucgaacugga ucccccuuuc ggcgacagcu acaucguuau uggggugggc gauaagaaaa     1800

ucacccacca cuggcauaga uccggaucua cgcuuggcaa ggccuuuagc acaacccuga     1860

aaggggcuca acgccucgcc gcgcugggag acacagccug ggauuucggc uccauugggg     1920

gagucuuuaa uucuaucggc cgggcugugc accagguuuu cgggggcgcc uuuagaaccc     1980

uguucggagg caugagcugg auuacccagg ggcuuauggg agcucuccug cuguggaugg     2040

gcgugaacgc ccgcgaccgg uccaucgccc ucgcuuuucu ggcgacaggg ggaguccuug     2100

uguuccuggc cacgaacguu caugcuugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10536
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10536
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggu gaccagacgg ggaagcgcuu acuauaugua ccuggaccgc aacgaugccg      180

gcgaagccau uuccuucccu acaacgcuug ggaugaauaa gugcuauauc caaauuaugg      240

accugggaca caugugugau gcuaccaugu cuuacgagug cccgaugcuc gacgaaggcg      300

uggagcccga ugacgucgau uguuggugca acacaaccag cacaugggug guuuauggga      360

ccugucauca caaaaaggga gaagccagac ggucccgcag agcugugaca cugccuucuc      420

auagcacccg gaaacuucag acgcgcuccc agaccuggcu ggagagcaga gaguacacaa      480

agcaccucau ccgggucgaa aauuggaucu uucgcaaccc cggcuucgcc cuggcggccg      540

cugccauugc uuggcugcuc gggucuucca ccagccagaa agugaucuau cugguuauga      600

uucuucugau cgccccugcg uacucuauua gaugcaucgg gguguccaau cgggacuuug      660

ucgagggaau gagcggcggg acaugggugg augugguccu cgaacaugga ggcuguguua      720

ccgugauggc ccaagacaag cccacggugg auauugagcu ggucacaacc acagugucua      780

acauggcuga gguucgcucc uacugcuaug aagccagcau cuccgacaug gcuucugaua      840

gcagaugucc uacccagggg gaggccuacc uugacaagca guccgacacc caguaugugu      900

gcaaacggac acuggucgau cgcggauggg gcaauggcug uggccucuuc ggaaagggca      960

gccuggugac gugcgccaaa uuugcuugcu cuaagaaaau gaccgggaag uccauccaac     1020

cggaaaaccu ggaguacaga auuaugcuca gcguucacgg aucucagcac agcggcauga     1080

ucgugaauga cacagggcau gaaaccgaug agaaccgggc gaaggucgag auuacaccca     1140

auuccccuag agccgaagcu acccugggag gcuucggcuc ucuugggcug gacugugagc     1200

cccgcacggg acucgauuuu agcgaccugu auuaccuuac aaugaacaau aaacacuggc     1260

uggugcauaa ggaaugguuc cacgauaucc cucuccccug gcacgccggc gccgacaccg     1320

ggaccccgca uuggaacaau aaagaggcuc uggucgaauu uaaggaugcc cacgcgaaac     1380

ggcagacagu gguugugcug ggaucccagg agggcgcugu ccauaccgcc cucgccgggg     1440

cucuggaggc cgaaauggac ggcgcuaagg gaagacuuag cucuggccac cugaaaugcc     1500

gccucaagau ggauaagcug cggcuuaaag ggguguccua uagccugugu acagccgcgu     1560

ucacguuuac caagauuccu gccgagacac uccauggaac cguuacagug gaaguccaau     1620

acgcuggcac cgacgggccc ugcaaagugc cugcccagau ggcuguggac augcagacgc     1680

ugacccccgu uggaagacug aucacagcca accccgucau caccgagucu acagaaaacu     1740

ccaagaugau gcucgagcug gauccuccgu ucggcgacag cuacauugug aucggggugg     1800

gcgagaaaaa gauuacccac cacuggcauc gcuccggauc uacgcuuggc aaggcguuca     1860

gcacaacccu gaaaggggcc caacggcucg cugcccuggg agauacagcu ugggacuuug     1920

gcuccaucgg gggagucuuc aauucuauug gcagagccgu gcaccagguu uuugggggcg     1980

ccuuccgcac ccuguuugga ggcaugagcu ggaucaccca ggggcuuaug ggagcucucc     2040

ugcuguggau gggcgugaac gcgcgggaua gauccaucgc ccucgcuuuc cuggccacag     2100

ggggaguccu uguguuucug gccacgaaug uucaugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10537
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10537
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcguu acgcggcgcg      120

gcuccgcgua cuauauguac cuggauagaa acgacgcugg ggaggccauu ucuuuuccca      180

ccacacuugg aaugaauaag ugcuauaucc agauuaugga ucugggccau augugugacg      240

ccaccaugag cuacgaaugc ccuaugcucg augagggggu ggaaccggac gauguggacu      300

guuggugcaa cacaaccucc acaugggucg uguauggaac cugucaccau aaaaagggcg      360

aggcucggcg cucuagacgg gccguuacgc ugcccagcca cuccacccgc aaacuucaaa      420

caagaucuca gaccuggcug gaaagccggg aguacacaaa gcaucucauc cgcguggaga      480

auuggauuuu cagaaacccu ggguuugcuc uggccgcggc cgcuaucgcc uggcugcucg      540

gauccagcac cucucagaaa gucaucuauc uggugaugau ucuucugauc gcucccgccu      600

acuccauucg gugcaucggc guuagcaauc gcgauuucgu ggaaggcaug ucugggggaa      660

cgugggucga cauugugcuc gagcacggcg ggugugugac agucauggcg caggauaagc      720

cuaccguuga caucgaacug gugacaacca ccguguccaa cauggccgag gucagaagcu      780

acugcuauga ggcuucuauu uccgauaugg ccagcgacuc ccgguguccc acacaaggag      840

aggcuuaccu ugacaagcag ucugauacgc aguaugugug caaacgcacc cugguugaca      900

gaggcugggg gaauggaugu ggccucuuug gcaaggggag ccuggugaca ugcgccaaau      960

ucgccugcuc caagaaaaug accggaaaga gcauccagcc ugaaaaccug gaauaccgga     1020

ucaugcucuc uguccacggc ucccaacaua gcgggaugau ugugaaugau acaggacacg     1080

agaccgacga aaacagagcu aagguugaga ucacgcccaa uucuccccgc gcggaggcca     1140

cacugggcgg guuuggaagc cuuggccugg auugugaacc ucggaccggc cucgacuucu     1200

ccgaucugua uuaccuuacc augaacaaua aacauuggcu ggugcacaag gagugguuuc     1260

acgacauucc ccucccuugg caugcugggg ccgauacagg aaccccgcac uggaacaaua     1320

aagaagcccu ggucgaguuc aaggacgcuc augccaaaag acagacagug gucgugcugg     1380

gcucucagga aggggcgguu cacacggcuc ucgccggagc ccuggaggcu gagauggaug     1440

gcgccaaggg gcgccuuagc uccggccauc ugaaaugccg gcucaagaug gacaagcuga     1500

gacuuaaagg agugagcuau ucucugugua ccgcugccuu uacauucacc aagaucccgg     1560

cggaaacacu ccacggcacc gucacggugg agguucagua cgccgggacc gacggaccuu     1620

gcaaagugcc cgcucaaaug gccgucgaua ugcagacacu gaccccugug ggccgccuga     1680

uuacagcuaa ccccgugauc accgaaucca cggagaacag caagaugaug cucgaacugg     1740

aucccccuuu uggggacucu uacaucguua uuggagucgg cgagaaaaag aucacacacc     1800

auuggcaccg guccgggagc acccuuggca aggccuucuc cacaacccug aaaggagcgc     1860

agagacucgc cgcucugggc gacaccgccu gggauuucgg gucuauugga ggcguguuua     1920

auagcaucgg gcgcgcugug caucaagucu ucggaggcgc cuuucggaca cuguucgggg     1980

gcauguccug gauuacgcag ggacuuaugg gcgcccuccu gcuguggaug ggggugaacg     2040

cuagagaccg cucuaucgcg cucgccuuuc uggcuaccgg aggcguucuu guguuccugg     2100

ccacaaaugu ccacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10538
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10538
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcguu acgcggcgcg      120

gcuccgcgua cuauauguac cuggauagaa acgacgcugg ggaggccauu ucuuuuccca      180

ccacacuugg aaugaauaag ugcuauaucc agauuaugga ucugggccau accugugacg      240

ccacaaugag cuacgaaugc ccuaugcucg augagggggu ggaaccggac gauguggacu      300

guuggugcaa caccacaucc accugggucg uguauggaac gugucaccau aaaaagggcg      360

aggcucggcg cucuagacgg gccguuaccc ugcccagcca cuccacacgc aaacuucaaa      420

ccagaucuca gacauggcug gaaagccggg aguacaccaa gcaucucauc cgcguggaga      480

auuggauuuu cagaaacccu ggguuugcuc uggccgcggc cgcuaucgcc uggcugcucg      540

gauccagcac gucucagaaa gucaucuauc uggugaugau ucuucugauc gcucccgccu      600

acuccauucg gugcaucggc guuagcaauc gcgauuucgu ggaaggcaug ucugggggaa      660

caugggucga cguggugcuc gagcacggcg ggugugucac cguuauggcg caggauaagc      720

cuacagugga cauugaacug gugaccacca cagucuccaa cauggccgag gugagaagcu      780

acugcuauga ggcuucuauc uccgauaugg ccagcgacuc ccgguguccc acgcaaggag      840

aggcuuaccu ugacaagcag ucugauaccc aguauguuug caaacgcaca cugguggaca      900

gaggcugggg gaauggaugu ggccucuuug gcaaggggag ccuggugacc ugcgccaaau      960

ucgccugcuc caagaaaaug acaggaaaga gcauucagcc ugaaaaccug gaauaccgga     1020

ucaugcucuc uguccacggc ucccaacaua gcgggaugau cguuaaugau accggacacg     1080

agacggacga aaacagagcu aagguggaga uuacacccaa uucuccccgc gcggaggcca     1140

cccugggcgg guuuggaagc cuuggccugg auugugaacc ucggaccggc cucgacuucu     1200

ccgaucugua uuaccuuaca augaacaaua aacauuggcu gguccacaag gagugguuuc     1260

acgacauccc ccucccuugg caugcugggg ccgauaccgg aacaccccac uggaacaaua     1320

aagaagcccu gguggaguuc aaggacgcuc augccaaaag acagacgguc gugguucugg     1380

gcucucagga aggggcggug cacaccgcuc ucgccggagc ccuggaggcu gagauggaug     1440

gcgccaaggg gcgccuuagc uccggccauc ugaaaugccg gcucaagaug gacaagcuga     1500

gacuuaaagg agucagcuau ucucugugua cagcugccuu uaccuucaca aagauuccgg     1560

cggaaacccu ccacggcacg gugaccguug aggugcagua cgccgggaca gacggaccuu     1620

gcaaaguccc cgcucaaaug gccguggaua ugcagacccu gacaccugug ggccgccuga     1680

ucaccgcuaa ccccguuauu acggaaucca cagagaacag caagaugaug cucgaacugg     1740

acccgccuuu uggggauucu uacaucguca ucggaguggg cgagaaaaag auuacccacc     1800

auuggcaccg guccgggagc acacuuggca aggccuucuc caccacccug aaaggagcgc     1860

agagacucgc cgcucugggc gacacagccu gggauuucgg gucuaucgga ggcguguuua     1920

auagcauugg gcgcgcuguc caucaagugu ucggaggcgc cuuucggacg cuguucgggg     1980

gcauguccug gaucacccag ggacuuaugg gcgcccuccu gcuguggaug gggguuaacg     2040

cuagagaccg cucuauugcg cucgccuuuc uggcuacagg aggcgugcuu gucuuccugg     2100

ccaccaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10539
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10539
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcauu acgcggcgcg      120

gcuccgcgua cuauauguac cuggauagau cugacgcugg gaaggccauc agcuuugcca      180

ccacacuugg aguuaacaaa ugccaugugc agauuaugga ucugggccac augugugacg      240

cuaccauguc cuaugagugc cccaugcucg augaaggggu ggagcccgac gaugucgacu      300

guuggugcaa uacaaccucu acaugggugg uuuacggaac cugucaucac aagaaaggcg      360

aagcccggcg cagcagacgg gcugugacgc ugccguccca uucuacccgc aaacuucaaa      420

caagaagcca gaccuggcug gagucccggg aauauacaaa gcaccucauc aaggucgaga      480

acuggauuuu ccgcaauccc ggguuugccc ugguggcggu ugccaucgcu uggcugcucg      540

gaagcucuac cucccagaag gugaucuacc uggucaugau ucuucugauc gccccugcuu      600

auagcauuag augcaucggc gugucuaacc gggauuucgu ggagggcaug uccgggggaa      660

cgugggucga cguugugcuc gaacacggcg ggugugugac agucauggcc caggauaaac      720

ccaccgugga cauugagcug guuacaacca ccgugagcaa uauggcggaa guccgcucuu      780

acugcuacga ggccuccauc agcgauaugg cuuccgacuc uagauguccu acacaaggag      840

aggccuaucu ugacaagcag agcgauacgc aguacgugug caaacggacc cugguugacc      900

gcggcugggg gaacggaugu ggccucuuug gcaagggguc ccuggugaca ugcgcuaaau      960

uuaccugcag caagaagaug acaggaaaau cuauucagcc cgaaaaucug gaguauagaa     1020

ucaugcucuc cguccauggc agccaacacu cugggaugau cggauacgaa accgaugagg     1080

accgggccaa gguggaaguc acgccuaaca gcccgagagc cgaggcuaca cugggcgggu     1140

uuggaucccu uggccuggau ugugagcccc gcaccggccu cgacuucucu gaucuguauu     1200

accuuaccau gaauaacaaa cauuggcugg ugcacaagga augguuucac gacauuccuc     1260

uccccuggca ugcgggggcc gauacaggaa ccccucacug gaauaacaaa gaggcucugg     1320

uugaguucaa ggacgcccau gccaaacggc agacaguggu cgugcugggc agccaggaag     1380

gggcuguuca cacggcccuc gcgggagcuc uggaagccga gauggauggc gccaagggga     1440

gacuuuucuc cggccaucug aagugccgcc ucaaaaugga caagcugcgg cuuaaaggag     1500

ugagcuauuc ucuguguacc gcugccuuca cauuuaccaa gguccccgcu gagacacucc     1560

acggcaccgu gacgguggaa guucaguacg ccgggaccga cggaccgugc aaaaucccug     1620

uccaaauggc gguggauaug cagacacuga cccccguggg cagacugauu acagccaauc     1680

cugucaucac cgaguccacg gaaaacagca agaugaugcu cgagcuggac ccucccuucg     1740

gggauucuua cauugugauc ggaguuggcg acaagaaaau cacacaccau uggcaccgcu     1800

ccgggagcac ccuuggcaag gcuuucucca caacccugaa aggagcccag cggcucgcug     1860

cccugggcga uaccgcgugg gacuuugggu cuauuggagg cguguucaau agcaucggga     1920

gagccgucca ucaaguguuu ggaggcgcuu uccgcacacu guuugggggc auguccugga     1980

uuacgcaggg acuuaugggc gcccuccugc uguggauggg gguuaacgcu cgggauagau     2040

cuaucgcccu cgccuuccug gcuaccggag gcgugcuugu cuuucuggcg acaaacgugc     2100

acgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10540
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10540
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcguu acgcggcgcg      120

gcuccgcgua cuauauguac cuggauagaa acgacgcugg ggaggccauu ucuuuuccca      180

ccacacuugg aaugaauaag ugcuauaucc agauuaugga ucugggccau augugugacg      240

ccaccaugag cuacgaaugc ccuaugcucg augagggggu ggaaccggac gauguggacu      300

guuggugcaa cacaaccucc acaugggucg uguauggaac cugucaccau aaaaagggcg      360

aggcucggcg cucuagacgg gccguuacgc ugcccagcca cuccacccgc aaacuucaaa      420

caagaucuca gaccuggcug gaaagccggg aguacacaaa gcaucucauc cgcguggaga      480

auuggauuuu cagaaacccu ggguuugcuc uggccgcggc cgcuaucgcc uggcugcucg      540

gauccagcac cucucagaaa gucaucuauc uggugaugau ucuucugauc gcucccgccu      600

acuccauucg gugcaucggc guuagcaauc gcgauuucgu ggaaggcaug ucugggggaa      660

cgugggucga cguggugcuc gagcacggcg ggugugucac aguuauggcg caggauaagc      720

cuaccgugga cauugaacug gugacaacca ccgucuccaa cauggccgag gugagaagcu      780

acugcuauga ggcuucuauc uccgauaugg ccagcgacuc ccgguguccc acacaaggag      840

aggcuuaccu ugacaagcag ucugauacgc aguauguuug caaacgcacc cugguggaca      900

gaggcugggg gaauggaugu ggccucuuug gcaaggggag ccuggucaca ugcgccaaau      960

ucgccugcuc caagaaaaug accggaaaga gcauucagcc ugaaaaccug gaauaccgga     1020

ucaugcucuc ugugcacggc ucccaacaua gcgggaugau cguuaaugau acaggacacg     1080

agaccgacga aaacagagcu aagguggaga uuacgcccaa uucuccccgc gcggaggcca     1140

cacugggcgg guuuggaagc cuuggccugg auugugaacc ucggaccggc cucgacuucu     1200

ccgaucugua uuaccuuacc augaacaaua aacauuggcu gguccacaag gagugguuuc     1260

acgacauccc ccucccuugg caugcugggg ccgauacagg aaccccgcac uggaacaaua     1320

aagaagcccu gguggaguuc aaggacgcuc augccaaaag acagacaguc gugguucugg     1380

gcucucagga aggggcggug cacacggcuc ucgccggagc ccuggaggcu gagauggaug     1440

gcgccaaggg gcgccuuagc uccggccauc ugaaaugccg gcucaagaug gacaagcuga     1500

gacuuaaagg agucagcuau ucucugugua ccgcugccuu uacauucacc aagauuccgg     1560

cggaaacacu ccacggcacc gugacgguug aggugcagua cgccgggacc gacggaccuu     1620

gcaaaguccc cgcucaaaug gccguggaua ugcagacacu gaccccugug ggccgccuga     1680

ucacagcuaa ccccguuauu accgaaucca cggagaacag caagaugaug cucgaacugg     1740

aucccccuuu uggggacucu uacaucguca ucggaguggg cgagaaaaag auuacacacc     1800

auuggcaccg guccgggagc acccuuggca aggccuucuc cacaacccug aaaggagcgc     1860

agagacucgc cgcucugggc gacaccgccu gggauuucgg gucuaucgga ggcguguuua     1920

auagcauugg gcgcgcuguc caucaagugu ucggaggcgc cuuucggaca cuguucgggg     1980

gcauguccug gaucacgcag ggacuuaugg gcgcccuccu gcuguggaug gggguuaacg     2040

cuagagaccg cucuauugcg cucgccuuuc uggcuaccgg aggcgugcuu gucuuccugg     2100

ccacaaaugu gcacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10541
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10541
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccguuac ccggcgcggc agcgccuacu auauguaccu ggacagaaac gaugcugggg      180

aggccaucuc cuuccccaca acgcuuggaa ugaauaaaug uuauauucag aucauggacc      240

ugggccacau gugcgaugcu accaugucuu acgaaugucc uaugcucgac gagggggugg      300

aaccggauga cguggauugc ugguguaaca caaccagcac augggucgug uauggaaccu      360

gccaucacaa gaaaggcgag gcccggcgcu ccagacgggc gguuacacug cccucucaua      420

gcacccgcaa acuucaaacg agaucccaga ccuggcugga aagccgggag uacacaaaac      480

accucauucg cguggagaau uggaucuuua gaaacccugg cuucgcccug gcugccgcug      540

ccaucgcgug gcugcucggg ucuuccacca gccagaaggu cauuuaucug gugaugaucc      600

uucugauugc ccccgcuuac ucuauccggu guauuggagu uuccaaucgc gacuuugugg      660

aaggcaugag cgggggaaca ugggucgaua ucgugcucga gcauggcggg ugcgugaccg      720

ucauggccca ggacaagccu acgguugaua uugaacuggu gacaaccaca gugucuaaca      780

uggcugaggu cagauccuac uguuaugagg ccagcaucuc cgacauggcc ucugauagcc      840

ggugccccac ccaaggagag gcuuaccuug acaaacaguc cgacacccag uaugugugua      900

agcgcacacu gguugauaga ggcuggggca augggugcgg acucuucggc aaagggagcc      960

uggugacgug cgcgaaguuu gccuguucua aaaagaugac cggaaagucc auccagccug     1020

aaaaccugga auaccggauu augcucagcg uccacggcuc ucaacacagc gggaugaucg     1080

ugaaugacac aggacaugag accgaugaaa acagagcuaa aguugagauu acacccaauu     1140

ccccgcgcgc cgaggccacc cugggcggcu ucgggucucu uggacuggac ugcgaaccuc     1200

ggacgggccu cgauuuuagc gaccuguauu accuuacaau gaacaauaag cacuggcugg     1260

ugcauaaaga gugguuccac gauauccccc ucccuuggca cgcuggggcc gacaccggaa     1320

caccccauug gaacaauaag gaagcgcugg ucgaguuuaa agaugcucac gccaagagac     1380

agaccguggu cgugcugggc ucccaggaag gggccguuca uaccgcucuc gccggcgcuc     1440

uggaggccga gauggacgga gcgaaaggcc gccuuagcuc ugggcaccug aagugucggc     1500

ucaagaugga uaaacugaga cuuaagggag uguccuauag ccugugcaca gccgcuuuca     1560

cguuuaccaa aauuccggcc gaaacacucc auggcaccgu cacaguggag guucaguacg     1620

cugggaccga cggaccuugu aaggugcccg cccaaauggc ggucgacaug cagacgcuga     1680

ccccuguggg ccgccugauc acagccaacc ccgugaucac cgaaucuaca gagaacucca     1740

aaaugaugcu cgaacuggau cccccuuucg gggacagcua cauuguuauc ggcgucggag     1800

agaagaagau uacccaccac uggcaucggu ccggcucuac gcuugggaag gcuuuuagca     1860

caacccugaa gggagcccag agacucgcug cccugggcga uacagccugg gacuucgggu     1920

ccaucggagg cguguucaau ucuauugggc gcgcugugca ccaagucuuu ggcggagcgu     1980

uccggacccu guuuggcggg augagcugga ucacccaggg acuuaugggc gcccuccugc     2040

uguggauggg ggugaacgcu agagaucgcu ccaucgcccu cgccuuccug gcuacaggag     2100

gcguucuugu guuucuggcc acgaaugucc augcgugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10542
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10542
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccguuac ccggcgcggc agcgccuacu auauguaccu ggacagaaac gaugcugggg      180

aggccaucuc cuuccccaca acgcuuggaa ugaauaaaug uuauauucag aucauggacc      240

ugggccauac cugcgaugcu acaaugucuu acgaaugucc uaugcucgac gagggggugg      300

aaccggauga cguggauugc ugguguaaca ccacaagcac cugggucgug uauggaacau      360

gccaccacaa gaaaggcgag gcccggcgcu ccagacgggc gguuacccug cccucucaua      420

gcacgcgcaa acuucaaacc agaucccaga cauggcugga aagccgggag uacaccaaac      480

accucauucg cguggagaau uggaucuuua gaaacccugg cuucgcccug gcugccgcug      540

ccaucgcgug gcugcucggg ucuuccacaa gccagaaggu cauuuaucug gugaugaucc      600

uucugauugc ccccgcuuac ucuauccggu guauuggagu uuccaaucgc gacuuugugg      660

aaggcaugag cgggggaacc ugggucgaug uggugcucga gcauggcggg ugcgucacgg      720

uuauggccca ggacaagccu acaguggaua ucgaacuggu gaccacaacc gucucuaaca      780

uggcugaggu gagauccuac uguuaugagg ccagcauuuc cgacauggcc ucugauagcc      840

ggugccccac ccaaggagag gcuuaccuug acaaacaguc cgacacacag uauguuugua      900

agcgcacgcu gguggauaga ggcuggggca augggugcgg acucuucggc aaagggagcc      960

uggugaccug cgcgaaguuu gccuguucua aaaagaugac aggaaagucc auccagccug     1020

aaaaccugga auaccggauc augcucagcg uccacggcuc ucaacacagc gggaugauug     1080

uuaaugacac cggacaugag acagaugaaa acagagcuaa aguggagauc acccccaauu     1140

ccccgcgcgc cgaggccacg cugggcggcu ucgggucucu uggacuggac ugcgaaccuc     1200

ggacaggccu cgauuuuagc gaccuguauu accuuaccau gaacaauaag cacuggcugg     1260

uccauaaaga gugguuccac gauauucccc ucccuuggca cgcuggggcc gacaccggaa     1320

caccccauug gaacaauaag gaagcgcugg uggaguuuaa agaugcucac gccaagagac     1380

agaccgucgu gguucugggc ucccaggaag gggccgugca uacagcucuc gccggcgcuc     1440

uggaggccga gauggacgga gcgaaaggcc gccuuagcuc ugggcaccug aagugucggc     1500

ucaagaugga uaaacugaga cuuaagggag ucuccuauag ccugugcacg gccgcuuuca     1560

ccuuuacaaa aaucccggcc gaaacccucc auggcacagu gaccguugag gugcaguacg     1620

cugggacgga cggaccuugu aagguccccg cccaaauggc gguggacaug cagacccuga     1680

caccuguggg ccgccugauu accgccaacc ccguuaucac agaaucuacc gagaacucca     1740

aaaugaugcu cgaacuggau cccccuuucg gggacagcua caucgucauu ggcgugggag     1800

agaagaagau cacgcaccac uggcaucggu ccggcucuac acuugggaag gcuuuuagca     1860

ccacacugaa gggagcccag agacucgcug cccugggcga uaccgccugg gacuucgggu     1920

ccauuggagg cguguucaau ucuaucgggc gcgcugucca ccaaguguuu ggcggagcgu     1980

uccggacccu guuuggcggg augagcugga uuacacaggg acuuaugggc gcccuccugc     2040

uguggauggg gguuaacgcu agagaucgcu ccaucgcccu cgccuuccug gcuacgggag     2100

gcgugcuugu cuuucuggcc accaaugugc augcgugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10543
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10543
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccaucac ccggcgcggc agcgccuacu auauguaccu ggacagaucc gaugcuggga      180

aagccauuuc uuucgcuaca acgcuuggag uuaacaagug ucacgugcag aucauggacc      240

ugggccauau gugcgaugcc accaugagcu augagugucc caugcucgac gaaggggugg      300

agcccgauga cgucgauugc ugguguaaua caaccuccac augggugguu uacggaaccu      360

gccaccauaa aaagggcgaa gcgcggcgcu cuagacgggc cgugacacug ccgagccacu      420

ccacccgcaa acuucaaacg agaagccaga ccuggcugga gucucgggaa uauacaaagc      480

aucucauuaa ggucgagaac uggaucuuuc gcaaucccgg cuucgcucug guggccguug      540

cuaucgccug gcugcucggg uccagcaccu cucagaaagu gauuuaccug gucaugaucc      600

uucugauugc gccugccuau uccaucagau guauuggagu gagcaaccgg gacuuugugg      660

agggcauguc ugggggaaca ugggucgaug uugugcucga acacggcggg ugcgugaccg      720

ucauggcuca ggacaagccc acgguggaua ucgagcuggu uacaaccaca guguccaaua      780

uggccgaagu ccgcagcuac uguuacgagg cuuccauuuc ugacauggcc agcgauucca      840

gaugcccuac ccaaggagag gccuaucuug acaaacagag cgacacccag uacgugugua      900

agcggacacu gguugaucgc ggcuggggca acgggugcgg acucuucggc aaagggucuc      960

uggugacgug cgcuaaguuu accuguucca agaaaaugac aggaaagagc auccagcccg     1020

aaaaucugga guauagaauc augcucucug uccacggcag ccaacauucc gggaugauug     1080

gauacgaaac cgacgaggau cgggcgaaag uggaagucac accuaacucu ccgagagccg     1140

aggcuacccu gggcggcuuc gggagccuug gacuggacug cgagccccgc acgggccucg     1200

auuuuuccga ccuguauuac cuuacaauga auaacaagca cuggcuggug cauaaagaau     1260

gguuccacga uaucccucuc cccuggcacg ccggggccga caccggaacc ccucauugga     1320

auaacaagga ggcucugguu gaauuuaaag augcccacgc gaagcggcag acaguggucg     1380

ugcugggcag ccaggagggg gcuguucaua ccgcccucgc cggcgcucug gaagccgaga     1440

uggacggagc uaagggcaga cuuuucucug ggcaccugaa augucgccuc aagauggaua     1500

aacugcggcu uaagggagug uccuauagcc ugugcacagc cgcguuuacg uucaccaaag     1560

uccccgccga gacacuccau ggcaccguga caguggaagu ucaguacgcu gggaccgacg     1620

gaccguguaa gauuccuguc caaauggccg uggacaugca gacgcugacc cccgugggca     1680

gacugaucac agcuaauccu gucauuaccg agucuacaga aaacuccaag augaugcucg     1740

agcuggaucc ucccuuuggg gacagcuaca ucgugaucgg cguuggagau aaaaagauua     1800

cccaccacug gcaucgcucc ggcucuacgc uugggaaagc cuucagcaca acccugaagg     1860

gagcgcagcg gcucgccgcu cugggcgaca cagccuggga uuucgggucc aucggaggcg     1920

uguuuaauuc uauugggaga gcuguccacc aaguguucgg cggagccuuu cgcacccugu     1980

ucggcgggau gagcuggauc acccagggac uuaugggcgc ccuccugcug uggauggggg     2040

uuaacgcucg ggacagaucc auugcgcucg ccuuucuggc uacaggaggc gugcuugucu     2100

uccuggccac gaacgugcau gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10544
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10544
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccguuac ccggcgcggc agcgccuacu auauguaccu ggacagaaac gaugcugggg      180

aggccaucuc cuuccccaca acgcuuggaa ugaauaaaug uuauauucag aucauggacc      240

ugggccacau gugcgaugcu accaugucuu acgaaugucc uaugcucgac gagggggugg      300

aaccggauga cguggauugc ugguguaaca caaccagcac augggucgug uauggaaccu      360

gccaucacaa gaaaggcgag gcccggcgcu ccagacgggc gguuacacug cccucucaua      420

gcacccgcaa acuucaaacg agaucccaga ccuggcugga aagccgggag uacacaaaac      480

accucauucg cguggagaau uggaucuuua gaaacccugg cuucgcccug gcugccgcug      540

ccaucgcgug gcugcucggg ucuuccacca gccagaaggu cauuuaucug gugaugaucc      600

uucugauugc ccccgcuuac ucuauccggu guauuggagu uuccaaucgc gacuuugugg      660

aaggcaugag cgggggaaca ugggucgaug uggugcucga gcauggcggg ugcgucaccg      720

uuauggccca ggacaagccu acgguggaua ucgaacuggu gacaaccaca gucucuaaca      780

uggcugaggu gagauccuac uguuaugagg ccagcauuuc cgacauggcc ucugauagcc      840

ggugccccac ccaaggagag gcuuaccuug acaaacaguc cgacacccag uauguuugua      900

agcgcacacu gguggauaga ggcuggggca augggugcgg acucuucggc aaagggagcc      960

uggucacgug cgcgaaguuu gccuguucua aaaagaugac cggaaagucc auccagccug     1020

aaaaccugga auaccggauc augcucagcg ugcacggcuc ucaacacagc gggaugauug     1080

uuaaugacac aggacaugag accgaugaaa acagagcuaa aguggagauc acacccaauu     1140

ccccgcgcgc cgaggccacc cugggcggcu ucgggucucu uggacuggac ugcgaaccuc     1200

ggacgggccu cgauuuuagc gaccuguauu accuuacaau gaacaauaag cacuggcugg     1260

uccauaaaga gugguuccac gauauucccc ucccuuggca cgcuggggcc gacaccggaa     1320

caccccauug gaacaauaag gaagcgcugg uggaguuuaa agaugcucac gccaagagac     1380

agaccgucgu gguucugggc ucccaggaag gggccgugca uaccgcucuc gccggcgcuc     1440

uggaggccga gauggacgga gcgaaaggcc gccuuagcuc ugggcaccug aagugucggc     1500

ucaagaugga uaaacugaga cuuaagggag ucuccuauag ccugugcaca gccgcuuuca     1560

cguuuaccaa aaucccggcc gaaacacucc auggcaccgu gacaguugag gugcaguacg     1620

cugggaccga cggaccuugu aagguccccg cccaaauggc gguggacaug cagacgcuga     1680

ccccuguggg ccgccugauu acagccaacc ccguuaucac cgaaucuaca gagaacucca     1740

aaaugaugcu cgaacuggau cccccuuucg gggacagcua caucgucauu ggcgugggag     1800

agaagaagau cacccaccac uggcaucggu ccggcucuac gcuugggaag gcuuuuagca     1860

caacccugaa gggagcccag agacucgcug cccugggcga uacagccugg gacuucgggu     1920

ccauuggagg cguguucaau ucuaucgggc gcgcugucca ccaaguguuu ggcggagcgu     1980

uccggacccu guuuggcggg augagcugga uuacccaggg acuuaugggc gcccuccugc     2040

uguggauggg gguuaacgcu agagaucgcu ccaucgcccu cgccuuccug gcuacaggag     2100

gcgugcuugu cuuucuggcc acgaaugugc augcgugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10545
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10545
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccguuacc cuucccuccc      120

acucuacaag aaagcugcag acccggagcc aaacauggcu cgagucccgc gaauacacca      180

aacaucugau uagaguggag aacuggaucu uccggaaucc uggauuugcg cuugcugccg      240

ccgcuauugc cuggcugcuc ggcucuagca caucccagaa ggugaucuau cuggucauga      300

uucugcucau cgcuccggcc uacucuaucc gcugcauugg ggugagcaac agagacuucg      360

uugaaggaau guccggcggg accugggugg auaucguccu ggagcacgga ggcuguguga      420

cgguuauggc gcaggacaaa cccaccgugg auauugaacu ugucacaacc acagugagca      480

auauggccga ggugcggucu uauugcuacg aggcuuccau cagcgacaug gccucugauu      540

cccgcugucc uacccagggc gaggcuuauc uggacaagca aagcgauacg caguacgucu      600

gcaaaagaac acucguugac cggggguggg gaaacggcug ugggcuguuu ggaaagggcu      660

cucuugugac cugcgccaag uucgcguguu ccaaaaagau gacagggaaa agcauucagc      720

ccgaaaaucu ggaauaucgc aucaugcucu ccgugcaugg aucucagcac agcggcauga      780

uugucaacga uaccggccau gagaccgacg agaauagagc caagguggaa aucacaccua      840

acagcccccg ggcugaggcc acgcuugggg gauuuggcuc ccuggggcuc gauugcgaac      900

cucgcaccgg acuggacuuc ucugaucuuu acuaccugac aaugaauaac aaacacuggc      960

ucguucacaa ggagugguuu caugacaucc cgcugcccug gcacgcuggc gccgacaccg     1020

ggacaccuca uuggaauaac aaggaagccc uuguggaguu caaagaugcu cacgcgaaga     1080

gacaaaccgu cgugguucug ggaucccagg agggcgccgu gcacacggcu cucgccggcg     1140

cccuggaagc ugagauggac ggggccaaag gacggcugag cucuggccau cucaaguguc     1200

gccugaaaau ggauaagcug agacugaaag gggucagcua uucccucugc acagcggcuu     1260

uuaccuucac caagauuccc gccgaaacac ugcacggaac cgugacaguc gaggugcagu     1320

acgccggcac ggacgggccu uguaagguuc ccgcucagau ggccguggau augcaaaccc     1380

uuacaccggu cggccggcug aucaccgcua auccugugau uacagaaucu accgagaaca     1440

gcaaaaugau gcucgagcug gaucccccuu uuggagacuc cuauaucguu auuggcgugg     1500

gggaaaagaa aaucacgcau cacuggcaua gaagcggauc uaccaucggc aaggccuucg     1560

aggcgacagu ccgcggggcc aaacggaugg cugugcuggg agacaccgcc ugggauuuug     1620

gcuccguugg gggcgcucuc aauagccugg gaaagggcau ucaccagauc uucggggccg     1680

cguucaaguc ucuuuuugga ggcauguccu gguucagcca gauucugauc gggacacucc     1740

ugauguggcu gggacuuaac accaaaaacg gcuccauuuc ucucaugugc cuggcccucg     1800

ggggcgugcu gaucuuucug agcacggcug ucuccgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10546
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10546
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccguuacc cuucccuccc      120

acucuacaag aaagcugcag acccggagcc aaacauggcu cgagucccgc gaauacacca      180

aacaucugau uagaguggag aacuggaucu uccggaaucc uggauuugcg cuugcugccg      240

ccgcuauugc cuggcugcuc ggcucuagca caucccagaa ggugaucuau cuggucauga      300

uucugcucau cgcuccggcc uacucuaucc gcugcauugg ggugagcaac agagacuucg      360

uugaaggaau guccggcggg accugggugg augucgugcu ggagcacgga ggcuguguua      420

cggugauggc gcaggacaaa cccaccgucg auaucgaacu ugugacaacc acagugagca      480

auauggccga gguccggucu uauugcuacg aggcuuccau uagcgacaug gccucugauu      540

cccgcugucc uacccagggc gaggcuuauc uggacaagca aagcgauacg caguacguuu      600

gcaaaagaac acucguggac cggggguggg gaaacggcug ugggcuguuu ggaaagggcu      660

cucuugugac cugcgccaag uucgcguguu ccaaaaagau gacagggaaa agcauccagc      720

ccgaaaaucu ggaauaucgc auuaugcucu ccguccaugg aucucagcac agcggcauga      780

ucgugaacga uaccggccau gagaccgacg agaauagagc caagguugaa auuacaccua      840

acagcccccg ggcugaggcc acgcuugggg gauuuggcuc ccuggggcuc gauugcgaac      900

cucgcaccgg acuggacuuc ucugaucuuu acuaccugac aaugaauaac aaacacuggc      960

ucgugcacaa ggagugguuu caugacaucc cgcugcccug gcacgcuggc gccgacaccg     1020

ggacaccuca uuggaauaac aaggaagccc uugucgaguu caaagaugcu cacgcgaaga     1080

gacaaaccgu gguugugcug ggaucccagg agggcgccgu ccacacggcu cucgccggcg     1140

cccuggaagc ugagauggac ggggccaaag gacggcugag cucuggccau cucaaguguc     1200

gccugaaaau ggauaagcug agacugaaag gggugagcua uucccucugc acagcggcuu     1260

uuaccuucac caagaucccc gccgaaacac ugcacggaac cgucacagug gagguucagu     1320

acgccggcac ggacgggccu uguaaggugc ccgcucagau ggccgucgau augcaaaccc     1380

uuacaccggu gggccggcug auuaccgcua auccuguuau cacagaaucu accgagaaca     1440

gcaaaaugau gcucgagcug gaucccccuu uuggagacuc cuauauugug aucggcgucg     1500

gggaaaagaa aauuacgcau cacuggcaua gaagcggauc uaccaucggc aaggccuucg     1560

aggcgacagu gcgcggggcc aaacggaugg cugugcuggg agacaccgcc ugggauuuug     1620

gcuccguugg gggcgcucuc aauagccugg gaaagggcau ccaccagauu uucggggccg     1680

cguucaaguc ucuuuuugga ggcauguccu gguucagcca gauccugauu gggacacucc     1740

ugauguggcu gggacuuaac gccaaaaacg gcuccaucuc ucucaugugc cuggcucucg     1800

ggggcguccu gauuuuucug agcaccgccg uguccgcuug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10547
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10547
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcaucggcau uaucgggcug cugcugacaa cggccauggc ugccgugacc cuucccuccc      120

acucuacaag aaagcugcag acccggagcc aaacauggcu cgagucccgc gaauacacca      180

aacaucugau uaaggucgag aacuggaucu ucagaaaucc uggauuugcg cuuguugcug      240

uggccauugc cuggcugcuc ggcucuagca caucccagaa agugaucuau cuggucauga      300

uccugcucau ugcuccggcc uacucuaucc ggugcauugg ggugagcaac cgcgacuucg      360

uugaaggaau guccggcggg accugggugg augucgugcu ggagcacgga ggcuguguua      420

cggugauggc ucaggacaag cccaccgucg auaucgaacu ugugacaacc acagugagca      480

auauggccga ggucagaucu uauugcuacg aggcguccau uagcgacaug gccucugauu      540

cccggugucc uacccagggc gaggcuuauc uggacaaaca aagcgauacg caguacguuu      600

gcaagcgcac acucguggac agaggguggg gaaacggcug ugggcuguuu ggaaagggcu      660

cucuugugac cugcgccaaa uucacauguu ccaagaaaau gaccggaaag agcauccagc      720

ccgaaaaucu ggaauaucgg auuaugcucu ccguccaugg gucucagcac agcggcauga      780

ucggcuacga gaccgaugag gaccgcgcua aaguggaagu uacaccuaac ucccccagag      840

ccgaggcgac gcucggggga uuuggcagcc uggggcugga uugcgaaccu cggaccggac      900

uggacuucuc ugaucuuuac uaucugacaa ugaauaacaa gcauuggcuc gugcacaagg      960

agugguuuca cgacaucccg cugcccuggc augccggcgc ugacaccggg acaccucacu     1020

ggaauaacaa agaagcccuu gucgaguuca aggaugcuca ugccaaacgc caaaccgugg     1080

uugugcuggg aucccaggag ggcgccgucc acacggcucu cgcgggcgcc cuggaagcug     1140

agauggacgg ggccaaggga agacuguuua gcggccaccu caaaugucgg cugaagaugg     1200

auaaacuucg ccugaagggg gugucuuaca gccucugcac agccgcuuuc accuuuacca     1260

agguccccgc cgaaacacug cauggaaccg ugacaguuga ggugcaguau gcgggcacgg     1320

acgggccuug uaaaauuccc guccagaugg cuguggauau gcaaacccuu acaccgguug     1380

gcagacugau caccgccaau ccugugauua cagaauccac cgagaacucu aagaugaugc     1440

ucgagcugga ucccccuuuc ggagacagcu acaucgucau uggcgugggg gacaaaaaga     1500

ucacgcacca uuggcaccgg uccggaagca ccaucggcaa agccuuugaa gcuacaguga     1560

gaggggccaa gcgcauggcu guucugggag auaccgccug ggacuucggc ucugucgggg     1620

gcguguucaa uucccucgga aagggcauuc aucagaucuu uggggcggcc uucaaaagcc     1680

uguuuggagg caugucuugg uucucccaga uucuuaucgg gacacugcuc guguggcugg     1740

gacugaacac caagaauggc agcauuuccc uuacgugccu cgcucucggg ggcgucauga     1800

ucuuucuguc uacagccgug agcgcuugag gacuagugca ucacauuuaa aagcaucuca     1860

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     1920

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     1980

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2100

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2158


<210>  10548
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10548
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgugggcau cgucgggcug cuccugacaa cggccauggc ugccguuacc cuucccuccc      120

acucuacaag aaagcugcag acccggagcc aaacauggcu cgagucccgc gaauacacca      180

aacaucugau uagaguggag aacuggaucu uccggaaucc uggauuugcg cuugcugccg      240

ccgcuauugc cuggcugcuc ggcucuagca caucccagaa ggugaucuau cuggucauga      300

uucugcucau cgcuccggcc uacucuaucc gcugcauugg ggugagcaac agagacuucg      360

uugaaggaau guccggcggg accugggugg augucgugcu ggagcacgga ggcuguguua      420

cggugauggc gcaggacaaa cccaccgucg auaucgaacu ugugacaacc acagugagca      480

auauggccga gguccggucu uauugcuacg aggcuuccau uagcgacaug gccucugauu      540

cccgcugucc uacccagggc gaggcuuauc uggacaagca aagcgauacg caguacguuu      600

gcaaaagaac acucguggac cggggguggg gaaacggcug ugggcuguuu ggaaagggcu      660

cucuugugac cugcgccaag uucgcguguu ccaaaaagau gacagggaaa agcauccagc      720

ccgaaaaucu ggaauaucgc auuaugcucu ccguccaugg aucucagcac agcggcauga      780

ucgugaacga uaccggccau gagaccgacg agaauagagc caagguugaa auuacaccua      840

acagcccccg ggcugaggcc acgcuugggg gauuuggcuc ccuggggcuc gauugcgaac      900

cucgcaccgg acuggacuuc ucugaucuuu acuaccugac aaugaauaac aaacacuggc      960

ucgugcacaa ggagugguuu caugacaucc cgcugcccug gcacgcuggc gccgacaccg     1020

ggacaccuca uuggaauaac aaggaagccc uugucgaguu caaagaugcu cacgcgaaga     1080

gacaaaccgu gguugugcug ggaucccagg agggcgccgu ccacacggcu cucgccggcg     1140

cccuggaagc ugagauggac ggggccaaag gacggcugag cucuggccau cucaaguguc     1200

gccugaaaau ggauaagcug agacugaaag gggugagcua uucccucugc acagcggcuu     1260

uuaccuucac caagaucccc gccgaaacac ugcacggaac cgucacagug gagguucagu     1320

acgccggcac ggacgggccu uguaaggugc ccgcucagau ggccgucgau augcaaaccc     1380

uuacaccggu gggccggcug auuaccgcua auccuguuau cacagaaucu accgagaaca     1440

gcaaaaugau gcucgagcug gaucccccuu uuggagacuc cuauauugug aucggcgucg     1500

gggaaaagaa aauuacgcau cacuggcaua gaagcggauc uaccaucggc aaggccuucg     1560

aggcgacagu gcgcggggcc aaacggaugg cugugcuggg agacaccgcc ugggauuuug     1620

gcuccguugg gggcgcucuc aauagccugg gaaagggcau ccaccagauu uucggggccg     1680

cguucaaguc ucuuuuugga ggcauguccu gguucagcca gauccugauu gggacacucc     1740

ugauguggcu gggacuuaac accaaaaacg gcuccaucuc ucucaugugc cuggcccucg     1800

ggggcguccu gauuuuucug agcacggcug uguccgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10549
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10549
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggc ugugacccug ccuagccacu ccacaagaaa gcugcaaacg cggucucaga      180

ccuggcugga aagccgcgag uacacaaaac aucucauuag aguggaaaac uggaucuucc      240

ggaauccggg auuugcccug gccgcugccg cuauugccug gcuucugggc uccucuacca      300

gccagaaggu caucuaucuc gugaugaucc ugcugauugc gcccgccuac uccauccgcu      360

gcauuggggu uagcaacaga gacuucgugg agggaauguc uggcgggaca ugggucgaua      420

ucgugcucga acacggaggc uguguuaccg ugauggcuca ggacaaaccu acagucgaua      480

uugagcuggu gaccacgacc guguccaaua uggccgaggu ccggagcuau ugcuacgagg      540

cuucuaucuc cgacauggcc agcgauucuc gcugucccac acaaggcgaa gcguaucuug      600

acaagcaguc cgauacccag uacguuugca aaagaacacu gguggaccgg ggguggggaa      660

acggcugugg gcucuuugga aagggcagcc uggugaccug cgccaaguuc gcuuguucca      720

aaaagaugac ggggaaaucu auucagccug aaaaucuuga guaucgcauc augcugagcg      780

uccauggauc ccaacacagc ggcaugaucg ugaacgauac aggccaugag accgacgaaa      840

auagagccaa gguugagauu acacccaacu cuccucgggc ugaagccacc cucgggggau      900

uuggcucccu ggggcuggau ugcgagccgc gcaccggacu cgacuucagc gaucuguacu      960

accuuacaau gaauaacaaa cacuggcugg ugcacaagga augguuucau gacauccccc     1020

ucccuuggca cgccggcgcu gacacgggga caccccauug gaauaacaag gaggcgcugg     1080

ucgaguucaa agaugcccac gcuaagagac agacaguggu ugugcuugga ucucaggaag     1140

gcgccgucca caccgcccug gcuggcgccc ucgaggcgga aauggacggg gcuaaaggac     1200

ggcugagcuc cggccaucug aagugucgcc ucaaaaugga uaagcugaga cuuaaagggg     1260

ugucuuauag ccugugcaca gccgccuuua ccuucacgaa gauuccugcu gagacacucc     1320

acggaaccgu caccguggaa guucaguacg ccggcacaga cgggcccugu aaggugccgg     1380

cucaaauggc cgucgauaug cagacccuga caccuguggg ccggcuuauc acggcgaauc     1440

ccguuauuac cgaguccaca gagaacagca aaaugaugcu ggaacucgac ccucccuuug     1500

gagauucuua uaucgugauc ggcgucgggg agaagaaaau uacccaucac uggcauagau     1560

ccggaagcac aaucggcaag gccuucgaag cuaccgugcg cggggccaaa cggauggcug     1620

ugcugggaga cacggccugg gauuucggcu cuguuggggg cgcgcuuaau ucccucggaa     1680

agggcauuca ccagaucuuu ggggccgcuu ucaagagccu guuuggaggc auguccuggu     1740

ucucucaaau ucuuaucggg acccugcuca uguggcuggg acugaacacc aaaaacggca     1800

gcaucucccu uaugugccuc gcccucgggg gcguccugau uuuucugucu accgcuguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10550
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10550
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggc ugugacccug ccuagccacu ccacaagaaa gcugcaaacg cggucucaga      180

ccuggcugga aagccgcgag uacacaaaac aucucauuag aguggaaaac uggaucuucc      240

ggaauccggg auuugcccug gccgcugccg cuauugccug gcuucugggc uccucuacca      300

gccagaaggu caucuaucuc gugaugaucc ugcugauugc gcccgccuac uccauccgcu      360

gcauuggggu uagcaacaga gacuucgugg agggaauguc uggcgggaca ugggucgaug      420

ugguucucga acacggaggc ugugugaccg ucauggcuca ggacaaaccu acaguggaua      480

ucgagcuggu gaccacgacc gucuccaaua uggccgaggu ucggagcuau ugcuacgagg      540

cuucuauuuc cgacauggcc agcgauucuc gcugucccac acaaggcgaa gcguaucuug      600

acaagcaguc cgauacccag uacgugugca aaagaacacu gguggaccgg ggguggggaa      660

acggcugugg gcucuuugga aagggcagcc uggugaccug cgccaaguuc gcuuguucca      720

aaaagaugac ggggaaaucu auccagccug aaaaucuuga guaucgcauu augcugagcg      780

uccauggauc ccaacacagc ggcaugaucg uuaacgauac aggccaugag accgacgaaa      840

auagagccaa gguggagauc acacccaacu cuccucgggc ugaagccacc cucgggggau      900

uuggcucccu ggggcuggau ugcgagccgc gcaccggacu cgacuucagc gaucuguacu      960

accuuacaau gaauaacaaa cacuggcugg uccacaagga augguuucau gacauucccc     1020

ucccuuggca cgccggcgcu gacacgggga caccccauug gaauaacaag gaggcgcugg     1080

uggaguucaa agaugcccac gcuaagagac agaccguugu gguccuugga ucucaggaag     1140

gcgccgugca caccgcccug gcuggcgccc ucgaggcgga aauggacggg gcuaaaggac     1200

ggcugagcuc cggccaucug aagugucgcc ucaaaaugga uaagcugaga cuuaaagggg     1260

ucucuuauag ccugugcaca gccgccuuua ccuucacgaa gaucccugcu gagacacucc     1320

acggaaccgu gaccguugaa gugcaguacg ccggcacaga cgggcccugu aaggucccgg     1380

cucaaauggc cguggauaug cagacccuga caccuguugg ccggcuuauu acggcgaauc     1440

ccgugaucac cgaguccaca gagaacagca aaaugaugcu ggaacucgac ccucccuuug     1500

gagauucuua uauugucauc ggcguggggg agaagaaaau cacccaucac uggcauagau     1560

ccggaagcac aauuggcaag gccuucgaag cuaccgugcg cggggccaaa cggauggcug     1620

uucugggaga cacggccugg gauuucggcu cugucggggg cgcgcuuaau ucccucggaa     1680

agggcaucca ccagauuuuu ggggccgcuu ucaagagccu guuuggaggc auguccuggu     1740

ucucucaaau ccuuauuggg acccugcuca uguggcuggg acugaacgcc aaaaacggca     1800

gcaucucccu uaugugccuc gcucucgggg gcgugcugau cuuucugucu acagccguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10551
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10551
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggc ugugacccug ccuagccacu ccacaagaaa acuucaaacg cggucucaga      180

ccuggcugga aagccgcgag uacacaaagc aucucauuaa gguggaaaac uggaucuuca      240

gaaauccggg auuugcccug gucgccgugg cuauugccug gcuucugggc uccucuacca      300

gccagaaagu uaucuaucuc gugaugaucc ugcugauugc ucccgccuac uccauccggu      360

gcauuggggu cagcaaccgc gacuucgugg agggaauguc uggcgggaca uggguugaug      420

ugguccucga acacggaggc ugugugaccg ugauggcgca ggacaagccu acagucgaua      480

ucgagcuggu uaccacgacc guguccaaua uggccgaggu gagaagcuau ugcuacgaag      540

ccucuauuuc cgacauggcu agcgauucuc ggugucccac acaaggcgag gcuuaucuug      600

acaaacaguc cgauacccag uacgucugca agcgcacacu gguggacaga ggguggggaa      660

acggcugugg gcucuuugga aagggcagcc ugguuaccug cgccaaauuc acguguucca      720

agaaaaugac agggaagucu auccagccug aaaaucuuga guaucggauu augcugagcg      780

ugcauggauc ccaacacagc ggcaugaucg gcuacgagac cgaugaagac cgcgcgaaag      840

ucgaggugac acccaacucu ccuagagccg aagcuacccu cgggggauuu ggcucccugg      900

ggcuggauug cgagccgcgg accggacucg acuucagcga ucuguacuau cuuacaauga      960

auaacaagca uuggcugguu cacaaggaau gguuucacga caucccccuc ccuuggcaug     1020

ccggcgcuga cacggggaca ccccacugga auaacaaaga ggcccuggug gaguucaagg     1080

augcccaugc uaaacgccag accgucgugg uccuuggauc ucaggaaggc gcggugcaca     1140

ccgcccuggc uggcgcccuc gaggccgaaa uggacggggc uaagggaaga cuguuuagcg     1200

gccaccugaa augucggcuc aagauggaua aacugcgccu uaaggggguu uccuacucuc     1260

ugugcacagc cgcguucacc uuuacgaagg ugccugcuga gacacuccau ggaaccguca     1320

ccguggaagu ucaguaugcc ggcacagacg ggcccuguaa aauuccggug caaauggccg     1380

ucgauaugca gacccugaca ccugugggca gacuuaucac ggcuaauccc gugauuaccg     1440

agagcacaga gaacuccaag augaugcugg aacucgaccc ucccuucgga gauagcuaca     1500

ucguuauugg cgucggggac aaaaagauca cccaccauug gcaccggucu ggauccacaa     1560

ucggcaaagc cuucgaggcu accgugagag gggccaagcg cauggcggug cugggagaua     1620

cggccuggga cuuuggcagc gucgggggcg uguucaauuc ucugggaaag ggcauucauc     1680

agaucuuugg ggcugccuuc aaaucccucu uuggaggcau gagcugguuc ucccaaauuc     1740

ugaucgggac ccuucugguu uggcucggac ugaacacaaa gaauggcucu auuagccuga     1800

ccugccuugc ucucgggggc gugaugaucu uucuguccac agccgucucu gcgugaggac     1860

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     1920

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     1980

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2040

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2160

ggcucuuuuc agagccacca gaauu                                           2185


<210>  10552
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10552
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu gcccguccug ggguucuuua uuaucgcugu ucucaugucc gcccaggagu      120

cuugggcggc ugugacccug ccuagccacu ccacaagaaa gcugcaaacg cggucucaga      180

ccuggcugga aagccgcgag uacacaaaac aucucauuag aguggaaaac uggaucuucc      240

ggaauccggg auuugcccug gccgcugccg cuauugccug gcuucugggc uccucuacca      300

gccagaaggu caucuaucuc gugaugaucc ugcugauugc gcccgccuac uccauccgcu      360

gcauuggggu uagcaacaga gacuucgugg agggaauguc uggcgggaca ugggucgaug      420

ugguucucga acacggaggc ugugugaccg ucauggcuca ggacaaaccu acaguggaua      480

ucgagcuggu gaccacgacc gucuccaaua uggccgaggu ucggagcuau ugcuacgagg      540

cuucuauuuc cgacauggcc agcgauucuc gcugucccac acaaggcgaa gcguaucuug      600

acaagcaguc cgauacccag uacgugugca aaagaacacu gguggaccgg ggguggggaa      660

acggcugugg gcucuuugga aagggcagcc uggugaccug cgccaaguuc gcuuguucca      720

aaaagaugac ggggaaaucu auccagccug aaaaucuuga guaucgcauu augcugagcg      780

uccauggauc ccaacacagc ggcaugaucg uuaacgauac aggccaugag accgacgaaa      840

auagagccaa gguggagauc acacccaacu cuccucgggc ugaagccacc cucgggggau      900

uuggcucccu ggggcuggau ugcgagccgc gcaccggacu cgacuucagc gaucuguacu      960

accuuacaau gaauaacaaa cacuggcugg uccacaagga augguuucau gacauucccc     1020

ucccuuggca cgccggcgcu gacacgggga caccccauug gaauaacaag gaggcgcugg     1080

uggaguucaa agaugcccac gcuaagagac agacaguugu gguccuugga ucucaggaag     1140

gcgccgugca caccgcccug gcuggcgccc ucgaggcgga aauggacggg gcuaaaggac     1200

ggcugagcuc cggccaucug aagugucgcc ucaaaaugga uaagcugaga cuuaaagggg     1260

ucucuuauag ccugugcaca gccgccuuua ccuucacgaa gaucccugcu gagacacucc     1320

acggaaccgu gaccguugaa gugcaguacg ccggcacaga cgggcccugu aaggucccgg     1380

cucaaauggc cguggauaug cagacccuga caccuguugg ccggcuuauu acggcgaauc     1440

ccgugaucac cgaguccaca gagaacagca aaaugaugcu ggaacucgac ccucccuuug     1500

gagauucuua uauugucauc ggcguggggg agaagaaaau cacccaucac uggcauagau     1560

ccggaagcac aauuggcaag gccuucgaag cuaccgugcg cggggccaaa cggauggcug     1620

uucugggaga cacggccugg gauuucggcu cugucggggg cgcgcuuaau ucccucggaa     1680

agggcaucca ccagauuuuu ggggccgcuu ucaagagccu guuuggaggc auguccuggu     1740

ucucucaaau ccuuauuggg acccugcuca uguggcuggg acugaacacc aaaaacggca     1800

gcaucucccu uaugugccuc gcccucgggg gcgugcugau cuuucugucu accgcuguga     1860

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10553
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10553
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcgcg guuacgcugc      120

ccucccauuc uacccggaaa cuucagacac gcagccaaac cuggcuggag uccagagaau      180

acacaaaaca ccucauucgg guggagaacu ggaucuuucg caauccuggc uucgcucugg      240

ccgccgcugc cauugcuugg cuucuggggu cuagcaccuc ccagaaggug aucuaucucg      300

ucaugauucu gcugaucgcc ccggcguacu cuaucagaug cauuggagug agcaaccggg      360

auuuuguuga aggcaugucc gggggaacau ggguggacau cguccucgag cauggcgggu      420

gugugaccgu uauggcccag gauaaaccca cgguggacau ugaacugguc accacaaccg      480

ugagcaauau ggcugaggug cgcucuuauu gcuacgaggc cuccaucagc gauauggcuu      540

cugacuccag auguccuaca cagggagaag ccuaucuuga uaagcaaagc gacacccagu      600

acgucugcaa acggacgcug guugaucgcg gcuggggcaa cgggugugga cucuucggca      660

aggggucucu ggugacaugc gcgaaguuug ccuguuccaa aaagaugacc ggaaaaagca      720

uucagcccga gaaucuugaa uauagaauca ugcuguccgu gcacggcucu cagcauagcg      780

ggaugauugu caacgacaca ggacacgaga ccgaugagaa ucgggcuaag guggaaauca      840

ccccuaacag cccccgcgcc gaggcuacac ucggcggcuu cgggucccug ggacuggacu      900

gcgaaccuag aacgggccuc gauuuuucug accuguacua ccuuaccaug aauaacaaac      960

acuggcuggu ucauaaggag ugguuccacg acaucccgcu ccccuggcau gccggggccg     1020

auacaggaac cccucacugg aauaacaagg aagcucuggu ggaguuuaaa gacgcgcacg     1080

ccaagcggca aacagucgug guucuuggcu cccaggaggg ggcugugcau accgcccugg     1140

ccggagcucu cgaagccgag auggauggcg cgaaaggccg ccugagcucu gggcaccuga     1200

aguguagacu caaaauggac aagcugcggc uuaaaggagu cagcuauucc cugugcacgg     1260

cugccuucac auuuaccaag auucccgccg aaacccucca uggcacagug accgucgagg     1320

ugcaguacgc ugggacagau ggaccuugua agguucccgc ccagauggcu guggacaugc     1380

aaacgcugac cccggucggc agacuuauca cagccaaucc ugugauuacc gaaucuacag     1440

agaacagcaa aaugaugcug gagcucgauc ccccuuucgg ggacuccuau aucguuauug     1500

gcgugggaga aaagaaaauc acccaccauu ggcaccgcag cggcucuacg aucgggaagg     1560

cguuugaggc caccguccgg ggagcuaaaa gaauggccgu gcugggcgau acagcuuggg     1620

acuucggguc cgugggaggc gcccugaaua gccucgggaa gggcauucac cagaucuucg     1680

gagcggccuu uaagucucug uucggcggga uguccugguu uagccagauu cuuaucggaa     1740

cccugcucau guggcugggc cugaacacaa agaacggguc cauuucucuu augugccucg     1800

cucugggagg cguucugauc uuccucagca ccgccgucuc cgcuugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10554
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10554
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcgcg guuacgcugc      120

ccucccauuc uacccggaaa cuucagacac gcagccaaac cuggcuggag uccagagaau      180

acacaaagca ccucauucgg guggagaacu ggaucuuucg caauccuggc uucgcucugg      240

ccgccgcugc cauugcuugg cuucuggggu cuagcaccuc ccagaaggug aucuaucucg      300

ucaugauucu gcugaucgcc ccggcguacu cuaucagaug cauuggagug agcaaccggg      360

auuuuguuga aggcaugucc gggggaacau ggguggacgu cgugcucgag cauggcgggu      420

guguuaccgu gauggcccag gauaaaccca cggucgacau cgaacuggug accacaaccg      480

ugagcaauau ggcugagguc cgcucuuauu gcuacgaggc cuccauuagc gauauggcuu      540

cugacuccag auguccuaca cagggagaag ccuaucuuga uaagcaaagc gacacccagu      600

acguuugcaa acggacgcug guggaucgcg gcuggggcaa cgggugugga cucuucggca      660

aggggucucu ggugacaugc gcgaaguuug ccuguuccaa aaagaugacc ggaaaaagca      720

uccagcccga gaaucuugaa uauagaauua ugcuguccgu ccacggcucu cagcauagcg      780

ggaugaucgu gaacgacaca ggacacgaga ccgaugagaa ucgggcuaag guugaaauua      840

ccccuaacag cccccgcgcc gaggcuacac ucggcggcuu cgggucccug ggacuggacu      900

gcgaaccuag aacgggccuc gauuuuucug accuguacua ccuuaccaug aauaacaaac      960

acuggcuggu gcauaaggag ugguuccacg acaucccgcu ccccuggcau gccggggccg     1020

auacaggaac cccucacugg aauaacaagg aagcucuggu cgaguuuaaa gacgcgcacg     1080

ccaagcggca aacagugguu gugcuuggcu cccaggaggg ggcuguccau accgcccugg     1140

ccggagcucu cgaagccgag auggauggcg cgaaaggccg ccugagcucu gggcaccuga     1200

aguguagacu caaaauggac aagcugcggc uuaaaggagu gagcuauucc cugugcacgg     1260

cugccuucac auuuaccaag auccccgccg aaacccucca uggcacaguc accguggagg     1320

uucaguacgc ugggacagau ggaccuugua aggugcccgc ccagauggcu gucgacaugc     1380

aaacgcugac cccggugggc agacuuauua cagccaaucc uguuaucacc gaaucuacag     1440

agaacagcaa aaugaugcug gagcucgauc ccccuuucgg ggacuccuau auugugaucg     1500

gcgucggaga aaagaaaauu acccaccauu ggcaccgcag cggcucuacg aucgggaagg     1560

cguuugaggc caccgugcgg ggagcuaaaa gaauggccgu gcugggcgau acagcuuggg     1620

acuucggguc cguuggaggc gcccugaaua gccucgggaa gggcauccac cagauuuucg     1680

gagcggccuu uaagucucug uucggcggga uguccugguu uagccagauc cuuauuggaa     1740

cccugcucau guggcugggc cugaacgcua aaaacggguc caucucucuu augugccucg     1800

cccugggagg cguccugauu uuccucagca cagcuguguc cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10555
<211>  2164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10555
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcgcg guuacgcugc      120

ccucccauuc uacccggaaa cuucagacac gcagccaaac cuggcuggag uccagagaau      180

acacaaaaca ccucauuaag guggagaacu ggaucuuucg gaauccuggc uucgcucugg      240

uggccgucgc cauugcuugg cuucuggggu cuagcaccuc ccagaaagug aucuaucucg      300

uuaugauucu gcugaucgcc ccggcuuacu cuauccgcug cauuggagug agcaacagag      360

auuuugucga aggcaugucc gggggaacau ggguggacgu ugugcucgag cauggcgggu      420

gugucaccgu gauggcccag gauaagccca cgguggacau cgaacugguc accacaaccg      480

uuagcaauau ggcggaggug cggucuuauu gcuacgaggc cuccauuagc gauauggcuu      540

cugacucccg cuguccuaca cagggagaag ccuaucuuga uaaacaaagc gacacccagu      600

acgugugcaa gagaacgcug gucgaucggg gcuggggcaa cgggugugga cucuucggca      660

aggggucucu ggugacaugc gcuaaauuua ccuguuccaa gaaaaugaca ggaaagagca      720

uccagcccga gaaucuugaa uaucgcauua ugcuguccgu ucacggcucu cagcauagcg      780

ggaugaucgg auacgagacc gacgaggaua gagccaaagu ggaagucacc ccuaacagcc      840

cccgggcgga ggccacacuc ggcggcuucg ggucccuggg acuggacugc gaaccucgca      900

cgggccucga uuuuucugac cuguacuauc uuaccaugaa uaacaagcac uggcuggugc      960

acaaggagug guuccaugac auuccgcucc ccuggcacgc uggggccgau acaggaaccc     1020

cucauuggaa uaacaaagaa gcucugguug aguuuaagga cgcccacgcc aaaagacaaa     1080

caguggucgu gcuuggcucc caggaggggg cuguccacac cgcgcuggcc ggagcucucg     1140

aagccgagau ggauggcgcc aagggccggc uguucagcgg gcaucugaaa ugucgccuca     1200

agauggacaa acugagacuu aagggagugu cuuacagccu gugcacggcu gccuuuacau     1260

ucaccaaggu ucccgcggaa acccuccacg gcacagugac cgucgaggug caguaugcug     1320

ggacagaugg accuuguaaa auccccguuc agauggccgu ggacaugcaa acgcugaccc     1380

cggucggccg gcuuaucaca gccaauccug ugauuaccga auccacagag aacucuaaga     1440

ugaugcugga gcucgauccc ccuuuugggg acagcuacau cgugauuggc guuggagaua     1500

aaaagaucac ccaucacugg cauagauccg gcagcacgau ugggaaggcu uucgaagcca     1560

ccguccgcgg agcuaagcgg auggccgugc ugggcgacac agcgugggau uucgggucug     1620

ugggaggcgu cuuuaauucc cuggggaagg gcauccacca gaucuucgga gccgcuuuua     1680

aaagccucuu cggcgggaug ucuugguuuu cccagauucu gaucggaacc cuucuggugu     1740

ggcucggccu gaacacaaag aaugggagca uuucccugac cugccuugcc cucggaggcg     1800

uuaugaucuu ccugucuacg gcugugagcg ccugaggacu agugcaucac auuuaaaagc     1860

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     1920

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     1980

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2160

aauu                                                                  2164


<210>  10556
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10556
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccuguuccuc guggccgcug ccacaagagu ccacagcgcg guuacgcugc      120

ccucccauuc uacccggaaa cuucagacac gcagccaaac cuggcuggag uccagagaau      180

acacaaaaca ccucauucgg guggagaacu ggaucuuucg caauccuggc uucgcucugg      240

ccgccgcugc cauugcuugg cuucuggggu cuagcaccuc ccagaaggug aucuaucucg      300

ucaugauucu gcugaucgcc ccggcguacu cuaucagaug cauuggagug agcaaccggg      360

auuuuguuga aggcaugucc gggggaacau ggguggacgu cgugcucgag cauggcgggu      420

guguuaccgu gauggcccag gauaaaccca cggucgacau cgaacuggug accacaaccg      480

ugagcaauau ggcugagguc cgcucuuauu gcuacgaggc cuccauuagc gauauggcuu      540

cugacuccag auguccuaca cagggagaag ccuaucuuga uaagcaaagc gacacccagu      600

acguuugcaa acggacgcug guggaucgcg gcuggggcaa cgggugugga cucuucggca      660

aggggucucu ggugacaugc gcgaaguuug ccuguuccaa aaagaugacc ggaaaaagca      720

uccagcccga gaaucuugaa uauagaauua ugcuguccgu ccacggcucu cagcauagcg      780

ggaugaucgu gaacgacaca ggacacgaga ccgaugagaa ucgggcuaag guugaaauua      840

ccccuaacag cccccgcgcc gaggcuacac ucggcggcuu cgggucccug ggacuggacu      900

gcgaaccuag aacgggccuc gauuuuucug accuguacua ccuuaccaug aauaacaaac      960

acuggcuggu gcauaaggag ugguuccacg acaucccgcu ccccuggcau gccggggccg     1020

auacaggaac cccucacugg aauaacaagg aagcucuggu cgaguuuaaa gacgcgcacg     1080

ccaagcggca aacagugguu gugcuuggcu cccaggaggg ggcuguccau accgcccugg     1140

ccggagcucu cgaagccgag auggauggcg cgaaaggccg ccugagcucu gggcaccuga     1200

aguguagacu caaaauggac aagcugcggc uuaaaggagu gagcuauucc cugugcacgg     1260

cugccuucac auuuaccaag auccccgccg aaacccucca uggcacaguc accguggagg     1320

uucaguacgc ugggacagau ggaccuugua aggugcccgc ccagauggcu gucgacaugc     1380

aaacgcugac cccggugggc agacuuauua cagccaaucc uguuaucacc gaaucuacag     1440

agaacagcaa aaugaugcug gagcucgauc ccccuuucgg ggacuccuau auugugaucg     1500

gcgucggaga aaagaaaauu acccaccauu ggcaccgcag cggcucuacg aucgggaagg     1560

cguuugaggc caccgugcgg ggagcuaaaa gaauggccgu gcugggcgau acagcuuggg     1620

acuucggguc cguuggaggc gcccugaaua gccucgggaa gggcauccac cagauuuucg     1680

gagcggccuu uaagucucug uucggcggga uguccugguu uagccagauc cuuauuggaa     1740

cccugcucau guggcugggc cugaacacaa agaacggguc caucucucuu augugccucg     1800

cucugggagg cguccugauu uuccucagca ccgccguguc cgcuugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10557
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10557
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccgccgu uacccugccc agccacucca cacggaaacu ucagacgcgc ucucaaaccu      180

ggcuggagag cagagaauac acaaagcauc ucauccgggu ggagaacugg auuuuccgca      240

auccuggcuu ugcucuggcc gcugccgcga ucgccuggcu ucuggggucc ucuaccagcc      300

agaaagugau uuaucucguc augauccugc ugaucgcucc ggccuacucc auuagaugua      360

ucggagugag caaccgggac uucguugaag gcaugucugg gggaacaugg guggauauug      420

uccucgagca cggcggcugc gugaccguua uggcucagga caagcccaca guggauaucg      480

aacuggucac cacgaccgug uccaauaugg ccgaggugcg cagcuauugu uacgaggcgu      540

cuauuuccga cauggccagc gauucuagau gcccuacaca gggggaggcu uaucuugaca      600

aacaauccga uacccaguac gucuguaagc ggacacuggu ugaccgcgga uggggcaacg      660

ggugcggacu cuuuggcaag gggagccugg ugaccugugc caaauucgcu ugcuccaaga      720

aaaugacggg aaagucuauc cagcccgaaa aucuugaaua uagaauuaug cugagcgugc      780

auggcuccca gcacagcggc augaucguca acgauacagg gcaugagacc gacgagaauc      840

gggccaaagu ggaaaucaca ccuaacucuc cccgcgccga ggcuacccuc ggaggcuuug      900

ggucccuggg acuggauugu gaaccuagaa ccggccucga cuucagcgau cuguacuacc      960

uuacaaugaa uaacaagcac uggcugguuc acaaggagug guuucaugac auuccgcucc     1020

ccuggcacgc gggggccgac acgggaaccc cucauuggaa uaacaaagaa gcucuggugg     1080

aguucaagga ugcccacgcc aaacggcaaa cagucguggu ucuuggcucu caggagggcg     1140

cugugcacac cgcccuggcg ggggcucucg aagccgagau ggacggagcc aagggccgcc     1200

ugagcuccgg gcaucugaaa ugcagacuca agauggauaa acugcggcuu aagggagucu     1260

cuuauagccu guguacagcu gccuuuaccu ucacgaagau ccccgcugaa acacuccacg     1320

gcaccgugac cgucgaggug caguacgccg ggacagacgg cccuugcaaa guucccgcgc     1380

agauggccgu ggauaugcaa acccugacac cggucggaag acuuauuacg gcuaauccug     1440

ugaucaccga auccacagag aacagcaaga ugaugcugga gcucgauccc ccuuuuggcg     1500

acucuuauau uguuaucggg gugggagaga aaaagaucac ccaucacugg caucgcuccg     1560

gcagcacaau ugggaaagcc uucgaagcua ccguccgggg agccaagaga auggcggugc     1620

ugggcgacac ggccugggau uuugggucug ugggcggagc ucucaauucc cugggcaagg     1680

ggauccacca gauuuucgga gccgcuuuca aaagccuguu uggcgggaug uccugguucu     1740

cucagauccu uauuggaacc cugcucaugu ggcugggccu gaacacaaag aacgggagca     1800

ucucccuuau gugccucgcc cugggcggag uucugaucuu ucucucuacc gccgucagcg     1860

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10558
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10558
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccgccgu uacccugccc agccacucca cacggaaacu ucagacgcgc ucucaaaccu      180

ggcuggagag cagagaauac acaaagcauc ucauccgggu ggagaacugg auuuuccgca      240

auccuggcuu ugcucuggcc gcugccgcga ucgccuggcu ucuggggucc ucuaccagcc      300

agaaagugau uuaucucguc augauccugc ugaucgcucc ggccuacucc auuagaugua      360

ucggagugag caaccgggac uucguugaag gcaugucugg gggaacaugg guggaugucg      420

ugcucgagca cggcggcugc guuaccguga uggcucagga caagcccaca gucgauauug      480

aacuggugac cacgaccgug uccaauaugg ccgagguccg cagcuauugu uacgaggcgu      540

cuaucuccga cauggccagc gauucuagau gcccuacaca gggggaggcu uaucuugaca      600

aacaauccga uacccaguac guuuguaagc ggacacuggu ggaccgcgga uggggcaacg      660

ggugcggacu cuuuggcaag gggagccugg ugaccugugc caaauucgcu ugcuccaaga      720

aaaugacggg aaagucuauu cagcccgaaa aucuugaaua uagaaucaug cugagcgucc      780

auggcuccca gcacagcggc augauuguga acgauacagg gcaugagacc gacgagaauc      840

gggccaaagu ugaaaucaca ccuaacucuc cccgcgccga ggcuacccuc ggaggcuuug      900

ggucccuggg acuggauugu gaaccuagaa ccggccucga cuucagcgau cuguacuacc      960

uuacaaugaa uaacaagcac uggcuggugc acaaggagug guuucaugac aucccgcucc     1020

ccuggcacgc gggggccgac acgggaaccc cucauuggaa uaacaaagaa gcucuggucg     1080

aguucaagga ugcccacgcc aaacggcaaa cagugguugu gcuuggcucu caggagggcg     1140

cuguccacac cgcccuggcg ggggcucucg aagccgagau ggacggagcc aagggccgcc     1200

ugagcuccgg gcaucugaaa ugcagacuca agauggauaa acugcggcuu aagggagugu     1260

cuuauagccu guguacagcu gccuuuaccu ucacgaagau ucccgcugaa acacuccacg     1320

gcaccgucac cguggagguu caguacgccg ggacagacgg cccuugcaaa gugcccgcgc     1380

agauggccgu cgauaugcaa acccugacac cggugggaag acuuaucacg gcuaauccug     1440

uuauuaccga auccacagag aacagcaaga ugaugcugga gcucgauccc ccuuuuggcg     1500

acucuuauau cgugauuggg gucggagaga aaaagaucac ccaucacugg caucgcuccg     1560

gcagcacaau cgggaaagcc uucgaagcua ccgugcgggg agccaagaga auggcggugc     1620

ugggcgacac ggccugggau uuugggucug uuggcggagc ucucaauucc cugggcaagg     1680

ggauucacca gaucuucgga gccgcuuuca aaagccuguu uggcgggaug uccugguucu     1740

cucagauucu uaucggaacc cugcucaugu ggcugggccu gaacgccaag aacgggagca     1800

uuucccuuau gugccucgcc cugggcggag uccugaucuu ucucucuaca gcugugagcg     1860

cgugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10559
<211>  2182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10559
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccgccgu uacccugccc agccacucca cacggaaacu ucagacgcgc ucucaaaccu      180

ggcuggagag cagagaauac acaaagcauc ucaucaaagu ggagaacugg auuuuccgga      240

auccuggcuu ugcucuggug gccgucgcua ucgccuggcu ucuggggucc ucuaccagcc      300

agaaggugau uuaucucguu augauccugc ugaucgcgcc ggccuacucc auucgcugua      360

ucggagugag caacagagac uucgucgaag gcaugucugg gggaacaugg guggauguug      420

ugcucgagca cggcggcugc gucaccguga uggcucagga caaacccaca guggauauug      480

aacuggucac cacgaccguu uccaauaugg ccgaggugcg gagcuauugu uacgaggcuu      540

cuaucuccga cauggccagc gauucucgcu gcccuacaca gggggaagcg uaucuugaca      600

agcaauccga uacccaguac guguguaaga gaacacuggu cgaccgggga uggggcaacg      660

ggugcggacu cuuuggcaaa gggagccugg ugaccugugc caaguucacg ugcuccaaaa      720

agaugacagg aaaaucuauu cagcccgaga aucuugaaua ucgcaucaug cugagcguuc      780

auggcuccca gcacagcggc augauugggu acgagaccga ugaggacaga gcuaaggugg      840

aagucacacc uaacucuccc cgggccgagg cuacccucgg aggcuuuggg ucccugggac      900

uggauuguga accucgcacc ggccucgacu ucagcgaucu guacuaucuu acaaugaaua      960

acaagcauug gcuggugcac aaagaguggu uucacgacau cccgcucccc uggcaugccg     1020

gggccgacac gggaaccccu cacuggaaua acaaggaagc ucugguugag uucaaagaug     1080

cgcaugccaa gagacaaaca guggucgugc uuggcucuca ggagggcgcu guccacaccg     1140

cccuggccgg ggcucucgaa gccgagaugg acggagcgaa aggccggcug uuuagcgggc     1200

accugaagug ccgccucaaa auggauaagc ugagacuuaa gggagugucc uacucucugu     1260

guacagcugc cuucaccuuu acgaaaguuc ccgccgaaac acuccauggc accgugaccg     1320

ucgaggugca guaugcuggg acagacggcc cuugcaagau ccccguucag auggccgugg     1380

auaugcaaac ccugacaccg gucggacggc uuauuacggc uaauccugug aucaccgaaa     1440

gcacagagaa cuccaaaaug augcuggagc ucgauccccc uuucggcgac agcuacauug     1500

ugaucggggu uggagacaag aaaauuaccc accauuggca cagaucuggc uccacaaucg     1560

ggaaggccuu ugaagcgacc guccgcggag ccaagcggau ggcugugcug ggcgauacgg     1620

ccugggacuu cgggagcgug ggcggagucu ucaauucucu gggcaaaggg auccaucaga     1680

uuuuuggagc ugccuucaag ucccucuuug gcgggaugag cugguucucc cagauccuga     1740

uuggaacccu ucuggugugg cucggccuga acaccaaaaa ugggucuauc agccugacau     1800

gccuugcgcu cggcggaguu augauuuuuc uguccacagc cgugucugcu ugaggacuag     1860

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     1920

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     1980

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2040

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2160

ucuuuucaga gccaccagaa uu                                              2182


<210>  10560
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10560
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agagaagcgc cggguccauc auguggcugg cuucucucgc cguggucauu gcgugcgcug      120

gagccgccgu uacccugccc agccacucca cacggaaacu ucagacgcgc ucucaaaccu      180

ggcuggagag cagagaauac acaaagcauc ucauccgggu ggagaacugg auuuuccgca      240

auccuggcuu ugcucuggcc gcugccgcga ucgccuggcu ucuggggucc ucuaccagcc      300

agaaagugau uuaucucguc augauccugc ugaucgcucc ggccuacucc auuagaugua      360

ucggagugag caaccgggac uucguugaag gcaugucugg gggaacaugg guggaugucg      420

ugcucgagca cggcggcugc guuaccguga uggcucagga caagcccaca gucgauauug      480

aacuggugac cacgaccgug uccaauaugg ccgagguccg cagcuauugu uacgaggcgu      540

cuaucuccga cauggccagc gauucuagau gcccuacaca gggggaggcu uaucuugaca      600

aacaauccga uacccaguac guuuguaagc ggacacuggu ggaccgcgga uggggcaacg      660

ggugcggacu cuuuggcaag gggagccugg ugaccugugc caaauucgcu ugcuccaaga      720

aaaugacggg aaagucuauu cagcccgaaa aucuugaaua uagaaucaug cugagcgucc      780

auggcuccca gcacagcggc augauuguga acgauacagg gcaugagacc gacgagaauc      840

gggccaaagu ugaaaucaca ccuaacucuc cccgcgccga ggcuacccuc ggaggcuuug      900

ggucccuggg acuggauugu gaaccuagaa ccggccucga cuucagcgau cuguacuacc      960

uuacaaugaa uaacaagcac uggcuggugc acaaggagug guuucaugac aucccgcucc     1020

ccuggcacgc gggggccgac acgggaaccc cucauuggaa uaacaaagaa gcucuggucg     1080

aguucaagga ugcccacgcc aaacggcaaa cagugguugu gcuuggcucu caggagggcg     1140

cuguccacac cgcccuggcg ggggcucucg aagccgagau ggacggagcc aagggccgcc     1200

ugagcuccgg gcaucugaaa ugcagacuca agauggauaa acugcggcuu aagggagugu     1260

cuuauagccu guguacagcu gccuuuaccu ucacgaagau ucccgcugaa acacuccacg     1320

gcaccgucac cguggagguu caguacgccg ggacagacgg cccuugcaaa gugcccgcgc     1380

agauggccgu cgauaugcaa acccugacac cggugggaag acuuaucacg gcuaauccug     1440

uuauuaccga auccacagag aacagcaaga ugaugcugga gcucgauccc ccuuuuggcg     1500

acucuuauau cgugauuggg gucggagaga aaaagaucac ccaucacugg caucgcuccg     1560

gcagcacaau cgggaaagcc uucgaagcua ccgugcgggg agccaagaga auggcggugc     1620

ugggcgacac ggccugggau uuugggucug uuggcggagc ucucaauucc cugggcaagg     1680

ggauucacca gaucuucgga gccgcuuuca aaagccuguu uggcgggaug uccugguucu     1740

cucagauucu uaucggaacc cugcucaugu ggcugggccu gaacacaaag aacgggagca     1800

uuucccuuau gugccucgcc cugggcggag uccugaucuu ucucucuacc gccgugagcg     1860

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10561
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10561
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

uaagcggagu accaguuuua gguuucuuua uaauagcugu cuuaauguca gcacaggaau      120

caugggcagu aacuagaaga gggucagccu auuauaugua ccuagauaga aaugacgcug      180

gugaagcgau aaguuuucca accacuuuag gcaugaauaa augcuauaua caaauaaugg      240

accuuggaca uaugugcgau gcuacaaugu cauacgaaug cccaauguua gaugaaggug      300

uagaaccaga ugacguagau ugcuggugua auacaacgag uacaugggua guauauggaa      360

cuugccauca uaagaaagga gaagcaagaa gaucaaggag agcaguuaca uuaccaucuc      420

auucuacuag aaaacuacaa accagaucuc aaacuugguu agaaaguaga gaauacacaa      480

aacaucuaau aagaguagaa aauuggaucu uuagaaaucc aggauuugca uuagcagcag      540

cagcaauagc auggcuauua ggaagcucaa caagucaaaa aguaauauac uuaguaauga      600

uacuucuaau agcaccagca uauucaauaa gauguauagg uguaucaaau agagauuuug      660

ucgaaggaau gucagguggu acauggguag auauagucuu agaacacgga ggauguguca      720

caguuauggc ucaagauaaa ccaacaguag auauugagcu aguaacaacg acaguaucaa      780

acauggccga agucagauca uacuguuaug aagcaucaau aagcgacaug gcaagugaua      840

guagaugucc gacacaagga gaagcauauc uagacaaaca aucagauaca caguauguau      900

gcaaaagaac auuaguagau agaggcuggg gaaacgguug cggauuauuu gguaaaggca      960

gccucguaac augcgcaaaa uuugcaugcu cgaagaaaau gacagguaaa aguauacaac     1020

cagaaaaccu agaauacaga aucauguuaa guguucaugg uagucaacau aguggaauga     1080

uaguuaacga uacaggacac gaaacugaug aaaauagagc aaaaguagaa auaacaccua     1140

auucuccaag agcagaagca acacuagggg gauuugguuc auuaggauua gacugugaac     1200

caagaacagg uuuagauuuu ucagauuuau auuaccucac aaugaauaac aaacauuggu     1260

uaguacauaa agaaugguuu caugauauuc cauugccuug gcaugccggu gcagacacag     1320

gcacaccaca uuggaauaau aaggaagcac uuguggaauu uaaagaugca caugcaaaaa     1380

gacaaacagu uguaguacuc gguucacaag aaggagcagu acacaccgca uuagcaggcg     1440

cacucgaggc agaaauggau ggggcaaaag ggagacuaag uaguggacau cugaaaugca     1500

gauuaaaaau ggacaaacua cgacuaaaag gaguaucaua uagucuuugc acagccgcgu     1560

ucacguucac uaaaauaccu gcugaaacau uacacggaac gguaacugua gaaguacaau     1620

augcaggaac agauggacca ugcaaaguuc cugcgcaaau ggcagucgac augcaaacau     1680

uaacaccagu agggagguua auuacagcua auccaguuau aacagaaagu acagagaaua     1740

guaaaaugau gcuagaauua gauccaccuu uuggggacuc uuacauagua auaggaguag     1800

gagaaaagaa aauaacccau cauuggcauc gaaguggaag uacaauagga aaagcauuug     1860

aggcaacagu aagaggagca aaaagaaugg caguacuugg agauacagcc ugggacuucg     1920

gcagugucgg aggagcacua aauaguuuag gaaaagguau acaucaaauu uuuggagccg     1980

ccuuuaaauc auuauucgga gggauguccu gguuuaguca aauauuaauu ggcacauuau     2040

uaauguggcu aggauuaaau accaaaaaug gcucaauauc auuaaugugu uuagcguuag     2100

guggaguacu aaucuuuuug agcacggcag ucagugccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10562
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10562
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcua       60

uaagcggagu accaguccua gguuucuuua uaaucgcugu cuuaauguca gcacaagaau      120

cgugggccgu aaccagaaga ggaagugcau auuauaugua uuuagaccga aaugaugcag      180

gcgaagcgau aucauuucca acgacauuag gaaugaauaa auguuauaua caaauaaugg      240

auuuaggaca cacaugcgau gcaaccaugu cuuacgaaug uccaauguua gacgaaggag      300

uagaaccaga ugacguagac uguuggugua auacaaccag uaccugggua guuuacggaa      360

caugucauca caagaaagga gaagcaagaa gaaguagacg agcaguaacc uugccaucac      420

auucaacaag aaaauuacaa acaagaucac aaacaugguu agaaagucga gaauauacaa      480

aacaucuaau aagaguagaa aacuggauau uuaggaaccc uggauuugca cucgcagcag      540

cugcaauugc augguugcua ggaagcucua cuucccaaaa aguuauuuau uuaguaauga      600

uauuauuaau agcaccagca uauucaauua gauguaucgg uguaucuaau agagacuuug      660

uagaaggaau gagcggaggu acuuggguag augucguauu agaacaugga ggauguguaa      720

caguaauggc ccaagauaaa ccaacgguag acauagaauu agucacuaca acuguuucaa      780

auauggcuga aguuagauca uacuguuaug aagcaaguau aucagauaug gccagugacu      840

ccagaugucc aacccaagga gaagcauauu uagacaaaca aagugacaca caauauguau      900

gcaaaagaac ucuaguagau agaggcuggg gaaauggaug cggauuauuu ggaaagggga      960

guuuaguaac augcgcaaaa uuugcaugcu caaagaaaau gacaggaaaa aguauccaac     1020

cagaaaauuu agaauauaga auaauguuau caguacaugg aucgcagcac agcggaauga     1080

uuguuaacga cacaggacau gaaacagaug aaaacagagc aaaaguugaa auuacaccaa     1140

acucaccaag agcagaagcg acauuaggag gauuuggauc acuagguuua gauugugaac     1200

ccagaacugg acuugauuuc agugacuuau auuaucuuac gaugaauaau aaacacuggc     1260

uaguucacaa agaaugguuu caugauauac cacuuccaug gcaugcagga gcagauaccg     1320

gcacaccuca cuggaacaau aaagaggccc uaguagaauu uaaagacgcu caugcaaaaa     1380

gacagacugu ugugguauua ggcucacaag aaggagcagu ucacacugcg cuagcagggg     1440

ccuuagaggc agaaauggac ggcgcaaaag guagacucuc aagcggacau uuaaaaugca     1500

gacugaaaau ggauaaauua cgacuaaaag gaguuaguua uagccuaugu acugccgcau     1560

uuacuuucac aaaaauacca gcagaaacac ugcauggaac uguaacggua gaaguacaau     1620

acgccgguac agacggaccu uguaaaguac cagcacaaau ggccguagau augcaaacac     1680

uaacuccugu ugguagacua auaacugcaa auccuguaau aacagaaagc acagaaaauu     1740

caaaaaugau guuagaauua gauccaccuu uuggagauuc auauauagua aucgggguug     1800

gagaaaagaa aauuacacac cauuggcauc gaucaggauc aacaaucggu aaagccuuug     1860

aagcgacagu aagaggugca aaaagaaugg caguauuagg agauacagca ugggauuuug     1920

guucgguagg aggagcauua aauucacuag gaaaagguau ucaccaaaua uuuggagcag     1980

ccuuuaaaag uuuauuugga ggaaugaguu gguuuaguca aauuuugaua ggaaccuuau     2040

uaauguggcu uggacucaac gcaaagaacg gaucaauuag cuuaaugugu cuagcguuag     2100

gaggaguuuu gauauuccua ucaacagcag uaagugcuug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10563
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10563
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaucaggagu accaguauua gguuuuuuca uaauagcagu acucauguca gcacaagaau      120

caugggcgau aacaagaaga ggaucagcau auuacaugua uuuagauaga ucagaugcgg      180

gaaaagcaau uuccuuugca acaacauuag gugugaacaa augccaugua caaauuaugg      240

aucuugguca caugugcgac gcaacaaugu cguacgaaug cccuaugcuu gacgaaggag      300

uugaaccaga ugacgucgau uguuggugua acacaaccuc aacauggguu gucuacggua      360

cuugucauca uaaaaaaggg gaagcaagga gaucaagaag agcuguaaca cuaccaaguc      420

auucaacuag aaaauuacaa acaagaagcc aaacauggcu agaaucucga gaauauacca      480

aacacuuaau aaaaguagaa aacuggauau uuaggaaucc aggauucgca uuagucgccg      540

uagccauugc cugguuacug ggaucuagua caagccaaaa agucauuuac cuaguaauga      600

uacuuuuaau agcaccagca uacucaauuc gguguauagg ugucaguaac agggauuuug      660

uugaagguau gaguggagga acauggguag auguuguacu cgaacacggc ggauguguca      720

caguaauggc acaagauaaa ccaacuguag acauagaacu aguaacaaca acuguaucaa      780

acauggcgga aguuagauca uacuguuaug aagcaaguau aucagauaug gcaucagauu      840

cacgcugccc aacacaaggc gaagcauauu uagauaaaca aagcgauaca caauauguau      900

gcaaacgaac acuaguagac agaggcuggg gcaauggaug cggauuauuc ggcaaaggaa      960

gucuuguaac cugugcaaaa uuuacaugcu caaaaaaaau gacagguaaa aguauacaac     1020

cagaaaaucu agaauaucga auaauguuaa gcguacaugg aucacaacau ucuggaauga     1080

uuggauauga aacggaugaa gauagagcaa aaguagaagu aacaccaaau uccccaagag     1140

cagaagccac acuagguggc uuuggaucuc uaggcuuaga uugugaacca agaacuggau     1200

uagauuuuuc agauuuauau uauuugacaa ugaauaauaa acauugguug guacauaaag     1260

aaugguuuca cgauauaccu uuaccauggc augcuggagc agacacagga accccacauu     1320

ggaacaauaa agaagcauua gucgaguuca aagaugccca ugcaaaaaga caaacgguag     1380

uaguucuagg aucccaagaa ggagcgguac auacagcacu cgcaggagca cuagaagccg     1440

aaauggaugg ugcuaaagga agauuauuuu caggacaucu uaaauguaga uuaaaaaugg     1500

auaaauuaag acuuaaagga guaucuuaua gucucuguac ugcggcuuuu acauuuacaa     1560

aaguaccagc agaaacguua caugguacag uaacaguaga aguacaauac gcaggaacag     1620

acgguccaug uaaaauuccu guacaaaugg caguagauau gcaaacucug acaccaguag     1680

guagauuaau aacagcuaau ccuguaauaa cagagucaac cgagaacuca aaaaugaugc     1740

uagaauuaga uccaccuuuu ggagauucau auauagucau aggcguagga gacaaaaaaa     1800

uaacacauca cuggcacaga ucaggaagua cuauagggaa ggcauuugaa gcaacgguaa     1860

gaggcgcaaa aagaauggca guauuaggag auaccgcaug ggauuuugga aguguuggug     1920

guguguuuaa cucuuuaggg aaagggauac aucaaauauu uggagcagca uuuaaaagcu     1980

uguuuggagg uaugucaugg uuuucacaaa uacuaauagg aacauugcua guaugguuag     2040

gauuaaauac uaaaaaugga agcauaagcu uaacaugcuu agcauuaggu ggaguaauga     2100

uauuuuuauc aacagcagua ucagcaugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10564
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10564
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcua       60

uuucuggggu uccaguauua ggauucuuua uaauagcagu auuaauguca gcacaagaaa      120

guugggcagu aacaagaaga ggaagugcau acuauaugua uuuggacaga aaugacgcug      180

gagaagccau aaguuuucca acaacauugg gcaugaacaa auguuauaua caaauaaugg      240

auuuaggaca uaugugugau gcaacaaugu cauacgaaug uccuaugcua gaugaaggag      300

ucgaaccaga cgauguagau uguuggugca auacaacuuc aacaugggua guuuauggaa      360

ccugucauca uaagaaaggg gaagcaagaa ggaguagaag ggcaguaacu uuacccucuc      420

auagcacaag aaaauuacaa acaagaucac aaacuuggcu cgaaagcaga gaauauacua      480

aacauuuaau aagaguagaa aauuggauuu ucagaaaucc aggauuugcu cuagcagcag      540

cagcaauagc augguuacuu gguuccagua caagucaaaa aguaaucuac cuuguaauga      600

uacuucuuau ugcuccagcg uauucaauaa gguguauugg aguaaguaac agagacuucg      660

uagaagguau gucuggaggc acguggguag acguaguauu agaacacggu ggguguguaa      720

cuguaauggc ucaagauaaa ccaacuguug auaucgaauu agugacaaca acaguuucaa      780

acauggcuga aguacgaagc uacugcuacg aagcaucaau aagugauaug gcaagugaua      840

gucgaugccc aacacaagga gaagcauauc uagacaaaca aagugauaca caauauguau      900

guaaaagaac acuuguagau agaggauggg gaaaugguug ugggcuauuu ggcaaaggga      960

gcuuagucac cugugcaaaa uuugcuugua gcaagaaaau gacaggaaaa agcauacaac     1020

cagaaaaucu agaauauaga auaauguuaa guguccaugg aucacaacau aguggaauga     1080

uaguaaauga caccggacac gaaacugacg aaaaucgagc aaaaguggaa auaacaccaa     1140

auagucccag ggccgaagcu acauuaggag guuuuggauc acuaggacug gauugugaac     1200

ccaggacagg guuagauuuc agcgauuuau auuauuuaac aaugaacaau aaacauuggu     1260

ugguacauaa agaaugguuu caugauauac cauuacccug gcaugcuggu gcugauacag     1320

gcacaccuca uuggaacaau aaagaagcau uaguagaauu uaaagaugcg caugcaaaaa     1380

gacaaacagu ugucguuuua ggaagucaag aaggcgcagu acauacugcu cuagcaggag     1440

cauuagaagc ugaaauggau ggagcaaaag gcagacuguc aaguggacau uuaaaauguc     1500

gacuaaaaau ggauaaauug cgauuaaaag gaguaucaua uuccuuaugc acugcagcuu     1560

uuacauuuac aaaaauaccu gcagaaacgu uacauggaac aguuacagua gaaguucaau     1620

acgccggaac agauggacca uguaaaguac cagcacaaau ggcaguagac augcaaacau     1680

uaacaccugu cggacgacua aucacagcaa auccaguuau uacagaauca acagaaaaua     1740

guaaaaugau gcuagaauua gauccaccau ucggagauuc auacauagua auaggaguug     1800

gagaaaagaa aauaacacac cauuggcauc gaagcggaag uacaauagga aaagcguucg     1860

aagcuacagu ccguggagca aaaagaaugg caguauuagg agauaccgca ugggauuuug     1920

gaaguguagg cggagcauua aauaguuuag gaaaagguau acaccaaauc uuuggagcag     1980

cauucaaauc acuauuugga ggaaugagcu gguuuucgca gauacugaua gguacuuuau     2040

uaauguggcu aggauuaaau acgaaaaacg guagcauauc auugaugugu uuagcacucg     2100

guggaguacu aauauuuuua ucuacagcug uauccgcgug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10565
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10565
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggacauggau uuuauuucua gucgcagcag cuacaagagu acacuccgua acaagaagag      120

gaucggcaua cuauauguau uuagacagaa acgaugcagg ggaagcaaua ucauuuccaa      180

caacucuugg uaugaauaaa uguuacauac agauaaugga uuuaggacac augugcgaug      240

ccacuauguc guacgagugc ccaauguuag acgaaggggu ggagccggau gauguagauu      300

gcugguguaa cacuaccucu acauggguag ucuauggaac augucaccac aagaaaggag      360

aagcaagaag aaguagacga gcaguaaccu uaccaaguca uucaacaaga aaauuacaaa      420

caagaucaca aacaugguua gaaucaaggg aauauacaaa acauuuaauu agaguagaaa      480

acuggauauu uagaaauccu gguuuugcuu uggcagcagc cgcuaucgcu ugguuauuag      540

gaagcagcac aucacaaaaa guaauauauu ugguaaugau uuuacucaua gcccccgcgu      600

auaguauucg auguauaggu guaucuaaua gagacuucgu agaaggaaug ucaggaggaa      660

cuuggguaga caucguauua gaacacggag gauguguaac aguaauggcu caagacaaac      720

cgacaguaga uauagaauug guaacaacaa cuguuucuaa uauggcagag guacgaucau      780

acuguuauga agcaagcauc agugauaugg cuucagacag ccgaugucca acacaaggug      840

aagcauacuu agauaaacaa agcgacacac aauauguaug caaaagaacg uuaguagaua      900

gaggcugggg aaaugguugc ggguuauucg gaaaaggauc auugguaaca ugcgcaaaau      960

uugcauguuc aaagaaaaug acuggaaaau cuauacaacc ugaaaaucua gaauauagga     1020

uaauguuaag uguucaugga ucucaacacu caggaaugau aguaaaugac acaggacacg     1080

aaacggauga aaauagagcc aaaguagaaa uaacaccaaa uucaccgaga gcagaagcga     1140

cauugggagg auuuggguca cuaggauuag auugugaacc gagaaccggg uuagauuuuu     1200

cugaucucua uuaucuaaca augaauaaua aacauugguu aguacacaaa gaaugguucc     1260

augauauucc auuaccuugg cacgcaggag ccgauacagg uacuccacac uggaacaaua     1320

aagaagcauu aguagaauuu aaagacgcac augcaaaaag acaaacagua guaguauuag     1380

gaagucaaga aggcgcagua cacaccgcuc uagcaggagc cuuagaagca gaaauggaug     1440

gagcaaaggg uagauugucu ucaggccauc uaaaauguag acuaaaaaug gauaaacuaa     1500

gauuaaaagg aguaagcuac ucuuuaugca cagcagcauu uacguuuaca aaaauaccag     1560

cagaaaccuu acauggaacu guuacuguag aaguacaaua ugcaggaaca gauggaccau     1620

guaaaguucc agcacaaaug gcaguagaua ugcaaacauu aacaccagua ggaagauuaa     1680

uuacagcaaa uccaguaauu acagaaucaa ccgaaaacuc aaaaaugaug uuagaauuag     1740

acccaccuuu cggagacuca uauauaguaa uaggaguagg agaaaagaaa auuacacacc     1800

auuggcaccg auccggauca acaauaggaa aagcauucga agcuacagua cgaggcgcaa     1860

aaaggauggc agucuuagga gauacagcau gggauuuugg aagcguagga ggagcacuua     1920

acuccuuagg uaaaggcaua caucaaauau uuggagcagc guuuaaauca uuauuugggg     1980

guaugucuug guuuagccaa auacucauag guaccuuauu aauguggcua ggacuuaaua     2040

cuaaaaacgg uucaauuuca uuaauguguu uagccuuagg agguguuuua auauuucuua     2100

guacagcagu uucagcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10566
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10566
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggacauggau auuauuuuua guggcagcag caacaagagu gcacucagug accaggagag      120

gaagugcaua uuauauguau uuagauagaa augacgcggg agaagcaaua ucauucccaa      180

cuacguuagg aaugaauaaa uguuauauac aaauaaugga uuuaggucau acaugugaug      240

caacaauguc uuacgaaugu ccgauguuag acgaaggagu agaaccagau gacguagacu      300

gcuggugcaa uacaacaagu acuuggguag uauacggaac augccaucac aaaaaaggag      360

aagcuagaag aucucgaagg gcaguaacuu uaccaaguca uucuacacga aaauugcaaa      420

ccaggucaca aacaugguua gaaucaaggg aauacacaaa acaucugaua agaguagaaa      480

acuggauauu uagaaauccc ggguuugcau uagcugcagc agcaauagca uggcuuuuag      540

guucaucaac aucucaaaaa guaauauauc uugugaugau acucuuaauu gcaccugcuu      600

acaguauuag guguauaggu gucucaaaua gagacuuugu cgaagggaug agugguggua      660

cauggguuga uguugucuug gaacauggug gauguguaac aguaauggca caagauaaac      720

caaccguaga uauagaauua gucacaacga caguaucuaa cauggcagaa guuagaucuu      780

auugcuauga agcgucaaua ucugauaugg ccucggacuc aagaugccca acacaaggcg      840

aagcauaucu agauaaacaa ucagauaccc aguauguaug uaaaagaaca uuaguugaua      900

gagguugggg aaaugguugc ggacuauuug gcaaaggauc auuaguaaca ugugcaaaau      960

ucgcauguag caaaaaaaug acaggaaaau caauucaacc agaaaauuug gaguauagaa     1020

uaaugcuaag ugugcacgga agccagcaua guggaaugau uguaaaugau acaggacaug     1080

aaacugacga gaauagagcg aaaguagaaa ucacaccaaa uucaccaaga gcugaagcua     1140

cacuaggagg auuugguagu uuaggccucg auugugaacc aagaacagga uuagacuucu     1200

ccgaucuaua uuauuuaaca augaauaaua aacacuggcu aguucauaaa gaaugguuuc     1260

augauauacc uuuaccuugg caugcaggag cagauacagg aacuccucau uggaacaaua     1320

aagaagcacu aguagaauuu aaagaugcac acgcaaaaag acaaacagua gucguacuag     1380

gaagucaaga aggagcaguu cauacagcuu uagcaggagc auuagaagca gaaauggaug     1440

gagcaaaagg gagauuauca ucaggacacc uaaaauguag auuaaaaaug gauaaauuaa     1500

gauuaaaagg aguaucauac ucauugugua cagcagcauu uacauuuaca aaaauaccag     1560

cagaaacauu acauggaaca gucacgguug aaguacaaua cgccggaacc gacggaccau     1620

gcaaaguacc ugcacaaaug gcaguagaua ugcaaacacu aacaccagua ggacgacuaa     1680

uaaccgcgaa uccaguaaua acagaaagua cagaaaauag caaaaugaug uuagaacuag     1740

auccuccauu uggagauuca uacauaguaa uuggagucgg ggaaaaaaag auuacucacc     1800

acuggcaucg aucgggcagu acaauaggaa aagcauuuga agcaacagua cgcggagcaa     1860

aaagaauggc aguacuagga gacaccgcau gggauuucgg uucaguaggu ggagcauuaa     1920

auucauuagg uaaaggaaua caccaaauau ucggagccgc cuuuaaaagu uuguuuggcg     1980

gaaugaguug guuuagccaa auauugaucg gaaccuuacu aauguggcug gguuuaaaug     2040

caaaaaaugg uagcauaagu cuaaugugcu uagcacuggg uggaguacua auauuuuuau     2100

caacggcagu aucagcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10567
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10567
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggaccuggau auuauuuuua guagcagcag cuacuagggu gcauucaaua acaagaagag      120

gaucagcuua cuauauguau uuagacagaa gcgacgcagg aaaagcaauc agcuuugcua      180

caacacuagg uguaaauaaa ugucauguac aaauaaugga uuuaggacau augugugaug      240

caacaaugag uuacgaaugu ccuauguuag augaaggugu ggaaccugac gauguagauu      300

guuggugcaa uacuacuagc acuuggguag ucuauggcac augucaucau aagaaaggcg      360

aagcaaggag aaguagacgu gcaguaacau ugccaucuca caguacacga aaauuacaaa      420

cuagaucaca gacauggcuu gaaucaagag aauauacaaa acacuuaaua aaaguagaaa      480

auuggauauu cagaaauccu ggguucgcac uuguggcugu cgcuauagcu ugguuacuag      540

gaaguaguac gagucaaaaa guuauauauc uuguuaugau auuacuaaua gcaccagcau      600

acaguauaag auguauaggg guuucuaaca gagacuuugu ugaaggaaug ucagguggaa      660

cauggguaga cguaguauua gaacacggag gauguguaac uguaauggca caagauaaac      720

caacuguuga uaucgaacuc guaacaacaa cagugagcaa uauggcggaa gugagaucau      780

acuguuauga ggcaucaauc ucagacaugg caucagauuc aagaugucca acucaaggug      840

aagcguaucu ggacaaacaa ucagacacac aauauguaug uaaaaggaca uuaguugaua      900

gaggaugggg uaauggaugc ggacuauucg gaaaagggag cuuaguaacc ugugcuaaau      960

uuacauguag uaaaaagaug acaggaaaau caauacaacc agaaaauuua gaauacagaa     1020

uaaugcuauc aguccauggc agucaacaca gcggaaugau ugguuaugaa acggacgagg     1080

auagagcuaa aguugaagua acgccuaaua guccuagagc agaagcuaca cuaggaggau     1140

uuggaucacu aggauuagac ugcgaaccac gaacaggauu agauuuuagu gauuuauauu     1200

accuaacgau gaauaacaaa cacugguuag uacacaaaga augguuucac gauauaccau     1260

uaccauggca ugcaggcgcu gauacaggaa caccacauug gaacaauaaa gaggcuuugg     1320

uagaauuuaa agacgcacau gcuaaaaggc agacaguagu aguauuagga ucacaagaag     1380

gagcugugca uacagcacuu gcaggagcac uagaagcaga aauggacggg gcaaaaggac     1440

gauuguucuc aggacauuua aaguguagac uaaaaaugga uaaacuaaga cuaaaaggug     1500

uaucauauag ccuauguacu gccgcauuua cguuuacuaa agucccagca gaaacucuac     1560

auggaaccgu aacugucgaa gugcaauaug cagguacaga uggcccuugu aaaauaccag     1620

uucaaauggc aguggacaug caaacauuaa caccgguagg aagauuaaua acagcaaauc     1680

cgguuauaac ggaaucaaca gaaaauagua aaaugaugcu agaauuggau ccaccauuug     1740

gugauucaua uauaguaaua ggcguaggag acaagaaaau aacacaucac uggcauagaa     1800

gcggaucaac aauagguaaa gcauuugaag cuacuguaag aggagcaaaa agaauggcag     1860

uccuagguga uacagcuugg gacuuuggua gcguaggagg aguguuuaau ucacucggaa     1920

aagguauuca ccaaaucuuu ggagccgcuu uuaaaucauu auucggaggu augagcuggu     1980

ucucacaaau ccuaauagga acacuauuag ugugguuggg acuaaauacu aaaaauggua     2040

gcauaucacu aacauguuua gcauuaggag ggguuaugau auuucuaagu acagcuguaa     2100

gcgcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10568
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10568
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggacauggau auuauuuuua guagcagcag caacgagagu acauagugua acaagacgag      120

guuccgcaua cuacauguau uuagauagaa augaugcggg agaagccaua ucauucccga      180

cgacauuagg aaugaauaaa uguuauaucc aaauaaugga uuuaggacac augugugaug      240

caacaauguc auaugaaugu ccaauguuag acgaaggagu ugaaccugau gauguagauu      300

guugguguaa cacaacuagc acguggguug ucuauggaac augucaucau aagaaaggag      360

aagcaagacg gaguaggaga gcaguaacau ugccaucaca caguacaaga aaacuacaaa      420

cacgaaguca aacaugguua gagagccgag aauauacaaa acaucuaaua agaguagaaa      480

auuggauuuu cagaaaucca gguuuugcau uagcagcagc agcaauagcc ugguuauuag      540

gaucaucaac aagucaaaaa guaauauauu uagucaugau cuuauuaaua gcaccagcgu      600

auucaauacg auguauugga guaucuaaca gggauuucgu agaagguaug ucaggaggaa      660

cauggguaga uguaguacuu gagcauggag gaugugugac aguaauggca caagauaaac      720

caacgguuga uauagaauua guaacaacaa caguaucaaa cauggcagaa guaagaaguu      780

auugcuauga agcaagcaua ucagauaugg cuucugacag ucgaugucca acacaaggag      840

aagcauauuu agacaaacaa ucugauacuc aauaugucug caaaagaaca uugguagaua      900

gggggugggg aaauggaugu ggauuguuug gaaaaggaag ucuaguaacg ugugcaaaau      960

uugcuugcuc gaaaaaaaug acuggaaaaa guauacaacc agaaaaucuu gaauauagaa     1020

uaauguuaag uguucaugga agucaacauu caggaaugau uguaaaugau acuggacaug     1080

aaacggacga aaauagggcc aaaguagaaa uaacaccuaa uaguccuaga gcugaagcca     1140

cauuaggagg auucggauca uuaggacucg acugugaacc aaggacagga cuagauuucu     1200

cugacuugua uuacuuaaca augaauaaua aacauugguu aguacacaaa gaaugguuuc     1260

acgauauacc acuaccaugg caugcgggag cugauacagg uacuccacac uggaauaaua     1320

aagaagcacu cguagaauuu aaagacgcac acgcaaaaag acaaacaguu guaguauuag     1380

guucacaaga gggcgcagua cauacagcau uagcaggugc uuuagaagcu gaaauggacg     1440

gagcaaaagg aagauuauca aguggacacc uaaaaugcag auugaaaaug gauaaauuac     1500

gauuaaaagg aguaucuuau agccuuugua cugccgcauu uaccuuuaca aaaauaccag     1560

cagaaacauu acauggaacu guaacaguag aaguacaaua cgcuggcacu gacggaccau     1620

guaaaguacc ggcucaaaug gcgguagaua ugcaaacauu aacaccagua ggucgccuaa     1680

uaacagcaaa uccaguaaua acugaaagca cugagaauuc caaaaugaug uuagaauugg     1740

auccaccuuu uggagauuca uacauaguaa uuggaguagg ugaaaaaaaa auaacacauc     1800

acuggcauag auccggauca acaauaggaa aagcauuuga agcaacaguu cguggggcua     1860

aaagaauggc aguacuagga gauacagccu gggauuucgg aucgguagga ggugcauuaa     1920

acucauuagg aaaagguaua caucaaauau uuggagcagc cuucaaaagu cucuuuggag     1980

gcaugaguug guucagucaa auauugauag gaacucuauu aauguggcuc ggcuuaaaua     2040

caaaaaaugg uucaauuuca uuaauguguu uagcauuagg aggaguauua auuuucuuau     2100

caaccgcagu auccgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10569
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10569
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaagaucagc uggaaguaua augugguuag caucauuagc uguaguaaua gcaugcgcag      120

gagcuguaac aagaagagga agcgcauauu auauguaccu agauagaaac gacgcaggag      180

aagcuaucuc uuuuccaaca acauuaggaa ugaauaaaug uuauauacaa auuauggauu      240

uaggacauau gugcgacgca acaaugucau augaaugucc uauguuagac gaaggaguag      300

aaccagacga uguagauugu ugguguaaua caacaaguac uuggguaguu uauggcacau      360

gccaucauaa gaaaggggaa gcucgaagaa gcagacgagc aguaacauua ccaagucaua      420

gcacaagaaa acuacaaaca agaagccaaa caugguuaga aucuagagaa uauacuaaac      480

auuuaauacg cguagaaaac uggauauuca gaaauccagg auuugcacua gcagcagcag      540

caauagcaug guuauuagga ucaaguacau cacaaaaagu aauauacuua guaaugauac      600

uauuaauagc accagcuuac ucaauaagau gcauaggagu aucaaaucgu gacuuuguag      660

aaggcaugag cgggggcaca uggguagaua uuguacuaga acaugguggu uguguuacag      720

uuauggcuca agauaaaccu accguugaua uagaguuagu aacaacaaca guaagcaaua      780

uggcagaagu aagaucauac uguuacgaag caucaauaag cgauauggca ucagauagua      840

gaugcccaac ucaaggagaa gcauacuuag auaaacaauc agauacacaa uauguaugca      900

agcgaacccu aguggaccga ggauggggaa auggaugugg acuauuugga aaaggcucuc      960

uuguuacaug ugcaaaauuu gcauguucua agaaaaugac aggaaaauca auacaaccug     1020

aaaacuuaga auacagaaua auguuaagcg uucauggaag ucaacacuca gguaugauag     1080

uaaaugauac aggacacgag accgaugaaa acagagcaaa aguagaaaua acacccaacu     1140

caccaagagc agaagcaacc cuuggcgguu uuggaaguuu aggauuagau ugugagccua     1200

gaacugguuu agauuucuca gauuuauauu auuuaacuau gaauaauaaa cauuggcuag     1260

uccauaaaga augguuucau gauauaccuu ugccauggca ugcgggugca gauaccggaa     1320

caccacacug gaacaacaaa gaagcacuug uagaauuuaa agaugcccac gcaaaaagac     1380

aaacuguagu aguacuugga agucaagaag gagcaguuca uacugcauua gcaggagcau     1440

uagaagcaga aauggaugga gccaaaggaa gauuaaguuc uggacaucuu aaauguaggu     1500

uaaaaaugga uaaauuaaga cuaaaaggcg uuucauacuc auuauguaca gcagcauuca     1560

ccuucacaaa aauaccagcu gaaacauuac acggaacagu aacaguugaa guucaauaug     1620

caggaacuga ugggccaugu aaaguaccug cacaaauggc aguagauaug caaacacuaa     1680

caccaguagg aagacuaaua acagcaaacc cuguaauuac ggaaucuaca gaaaacagca     1740

aaaugauguu agaguuggau ccacccuuug gagauagcua uauaguuaua ggagugggug     1800

aaaagaaaau aacgcaucau uggcacagaa gugguaguac aauaggaaaa gcauuugagg     1860

caaccguaag aggggcaaaa aggauggcag uccuaggaga cacagcuugg gacuuuggaa     1920

gcguaggcgg ggcccuuaau uccuuaggaa agggaauaca ccaaauauuu ggagcagcau     1980

uuaaaucauu auucgguggc augucuuggu uuucucaaau acuaauagga acauuauuaa     2040

uguggcuugg acuaaauacc aaaaauggcu caaucucauu aaugugucuu gcuuuaggag     2100

guguacuaau auuuuuaagu acagcaguaa gugcaugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10570
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10570
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aaagaucagc uggaucaaua augugguuag ccucacuagc aguuguuaua gcaugugcug      120

gagcgguaac uagaagaggu agugccuauu acauguaccu agauagaaac gaugcaggag      180

aagccauauc auuuccaaca acauuaggua ugaauaaaug cuauauucaa auaauggauu      240

uaggacacac augugaugca acaaugucuu augaaugucc aauguuagau gaaggaguug      300

aaccagauga uguagauugu ugguguaaua caacaaguac auggguugua uacggaacgu      360

gucaucauaa aaaaggagaa gcacgaagaa guagacgagc ugucacauua ccaagucaua      420

gcacucguaa auuacaaaca agaagucaaa cuuggcuaga aagcagagaa uauacgaagc      480

aucuaauaag gguugaaaac uggauauuca gaaacccagg auuugcuuua gcagcugcug      540

caauagcaug gcuauuagga agcucaacaa gucaaaaagu cauauauuua guaaugauac      600

uucuaauagc accagcauac aguauuagau gcauaggagu aaguaauagg gauuuuguag      660

aaggaaugag uggagguaca uggguugacg uaguauuaga acauggagga uguguaacag      720

uaauggcaca agacaaaccu acaguagaca uagaauuagu aacaacuaca guuucuaaua      780

uggccgaagu acgaagcuau uguuaugagg caucaauauc agauauggca ucagacucaa      840

gauguccaac ucaaggggaa gcguaucuag auaaacaauc cgacacccaa uacguaugua      900

aacgaacacu uguagauaga ggauggggaa acgguugugg auuauuuggu aaagguaguu      960

uaguaacuug cgcaaaauuu gcauguucaa aaaagaugac aggcaaauca auacaaccag     1020

aaaaccugga auauagaaua auguuaucag uacauggcuc acaacauagu gguaugaucg     1080

uuaacgauac uggacaugaa acagaugaaa aucgagcgaa aguagaaaua acgccaaauu     1140

caccuagagc ugaagcaaca uuaggaggau uugggagcuu aggacuugac ugugaaccua     1200

gaacaggauu agauuucagc gaucuauacu aucuuacgau gaauaauaaa cauuggcucg     1260

uacacaaaga gugguuucau gauauuccuc uuccauggca cgcaggagcu gauacuggga     1320

caccacauug gaauaauaaa gaagcuuuag uagaauucaa agaugcacac gcaaaaagac     1380

aaacaguagu uguguuagga ucucaagaag gagcgguaca uacagcauua gcaggagccc     1440

uagaagcaga gauggaugga gcaaaaggac gguuaucauc aggacaucua aaauguagac     1500

uuaaaaugga caaauuaaga cuaaaaggag uaucuuauuc auuaugcaca gcagcauuua     1560

ccuuuacaaa aauaccggca gaaacacuuc auggaacagu uacaguagaa guacaauacg     1620

caggaacaga uggcccaugc aaagucccag cacaaauggc uguagauaug caaacauuaa     1680

cuccaguagg uagacuuaua acagcaaauc cgguaauaac agagucaacg gaaaauucaa     1740

aaaugauguu agaacuagau ccaccauuug gagacaguua cauaguuauu ggcgucggag     1800

aaaaaaaaau aacacaucac uggcauagga gcggaaguac aauuggaaaa gcauucgaag     1860

cuacaguuag aggagccaaa agaauggcug uauuaggaga cacugcgugg gacuuuggaa     1920

guguaggagg cgcauuaaau agcuuaggua aaggaauaca ucaaauauuu ggugcggcau     1980

ucaaaucacu guucggagga augaguuggu uuucacaaau acuaaucgga acuuuacuaa     2040

ugugguuagg uuuaaaugca aaaaauggau caauaaguuu aaugugccug gcauuaggag     2100

gaguauugau auuuuuaagc acagcagugu cagcaugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10571
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10571
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaagaucagc agggaguaua augugguuag caagucuagc aguugucaua gcgugcgcag      120

gugcaaucac uagaagagga agcgcauauu acauguauuu agauagaagu gaugcaggaa      180

aagcaauuuc auuugcuaca acauuaggcg ucaauaaaug ucauguacaa aucauggauu      240

uaggucauau gugugaugca accaugagcu acgaaugucc aauguuagau gaaggaguag      300

aaccugauga cguagacugc ugguguaaua caacaaguac auggguagua uauggaacgu      360

gccaucacaa aaaaggagaa gcaagaagaa gccgaagagc ggucacccua ccaagucaua      420

gcacuagaaa auuacaaaca aggagucaaa cauggcuaga aagcagagaa uauacaaaac      480

acuuaauaaa aguagaaaau uggauauuuc guaauccagg cuucgcacua guagcaguag      540

ccauagcaug guugcuugga ucaucgacaa gucaaaaagu uaucuauuua guaaugauau      600

uauuaauagc gccugcauac ucaauacgau guauaggagu aucuaacaga gauuucguag      660

aaggaauguc uggagguacu uggguagaug uaguucugga acauggugga uguguuacug      720

uaauggccca agacaaacca accguagaua uugaacuagu aacuacuacu guaagcaaca      780

uggcggaagu acgaucauau uguuacgaag caaguauaag ugauauggca ucagauucua      840

gaugcccgac ccagggagaa gcauauuuag auaagcaaag ugacacccaa uacguaugua      900

aaagaacauu gguagauaga ggauggggaa acggaugugg auuauucggc aaaggaaguu      960

uaguaacaug ugcuaaauuu accugcagua aaaaaaugac aggcaaaagu auacaaccag     1020

aaaauuuaga auacagaaua augcuaagcg uacauggauc ucaacauuca ggaaugauag     1080

gauaugaaac agaugaagau agggcuaaag uagaagucac accuaauagc ccaagagcgg     1140

aagcgacccu ggguggguuu ggaucauuag gauuagauug cgaaccgaga accggauuag     1200

auuuuaguga uuuauauuau uuaacaauga acaauaaaca uugguuagug cauaaagagu     1260

gguuccauga cauaccacua ccauggcaug caggugcaga cacuggaaca ccacauugga     1320

auaauaaaga agcauuaguc gaauucaaag augcucaugc gaaaagacaa acaguagugg     1380

uucuaggauc ucaagaagga gcaguacaca cagcauuagc aggagcgcua gaagcagaaa     1440

uggauggcgc aaaaggaaga uuauucagcg gucauuuaaa auguagauua aaaauggaua     1500

aauuaagacu uaaaggagua ucauauucuc uaugcacagc agcauuuacu uuuacuaaag     1560

uaccagccga aacauuacau ggcacgguaa cugucgaggu acaauaugca gguacagacg     1620

gaccuuguaa aauaccagua caaauggcag uggauaugca aacauuaacu ccaguaggca     1680

ggcuaauaac agcuaaucca guaauaacag aaucaacuga aaacagcaaa augauguugg     1740

aauuagaucc accauuuggc gauuccuaua uaguaauagg gguaggagau aaaaaaauaa     1800

cacaccauug gcacaggagu ggaaguacaa uagggaaagc auuugaagcg acaguaagag     1860

gagcaaaaag gauggcagua cuaggagaua cagcauggga uuuugguagc gugggaggug     1920

uauuuaacuc acuaggcaag gggauacauc aaauauuugg cgcagcauuu aaaagcuuau     1980

uuggaggaau gaguugguuu ucacaaauuc ugauaggaac ucuuuuggua ugguuaggau     2040

uaaauacaaa aaacgguucu auaaguuuaa caugucuagc auuagggggu guaaugauau     2100

uucuaaguac ugcaguaagc gcaugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10572
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10572
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaagaucagc agguucaaua augugguuag caucauuagc gguaguaaua gcaugugcag      120

gagccguaac gcguagaggu ucagcuuauu auauguaucu ugauagaaac gaugcuggug      180

aagcaauauc auuuccaacc accuuaggaa ugaauaaaug uuauauacaa auaauggacc      240

uaggacauau gugugacgcg acaaugucau acgagugccc uauguuagac gaaggaguag      300

aaccagauga cguagauugu ugguguaaua caacgucaac guggguagua uauggcacau      360

gccaccauaa gaaaggugaa gcucgcagaa gcaggagagc aguuacauug ccaagccauu      420

cgacuagaaa auugcaaacc agaagucaaa caugguuaga aaguagagaa uauacuaaac      480

aucugauacg aguagagaac uggauauuua gaaauccagg auucgcguua gcagcggcag      540

caauagccug guuauuagga aguucaacaa gucagaaagu aauauaucua guaaugauac      600

uauuaauugc uccugcauau ucaauaagau guauaggagu aucaaauaga gauuuuguag      660

aaggaauguc uggcggaacu uggguagaug uaguacuaga acacgggggu ugugucacug      720

uaauggcaca agauaaacca acaguggaua uagaauuagu aaccacaaca guaaguaaua      780

uggcagaagu aagaucauau uguuaugaag caaguauaag ugacauggca ucagauagca      840

gauguccaac acaaggcgaa gcauacuuag auaaacaauc cgacacacaa uacguaugua      900

aaagaacauu agucgauaga ggauggggaa auggaugugg auuauuugga aaagggucau      960

ugguaacaug ugcaaaauuc gcauguagca agaaaaugac uggaaaauca auacaaccag     1020

aaaauuugga auaucgaaua auguuaagug uacauggcuc ucaacauuca gggaugauug     1080

uaaacgacac uggacaugaa acagaugaaa acagagcaaa aguagaaaua accccgaaua     1140

guccuagagc agaagcaaca cuagguggau uugggucauu agguuuggau ugugaaccaa     1200

gaacaggcuu agauuuuagu gaccuauauu accugacuau gaauaauaaa cauuggcuag     1260

uacauaaaga augguuucau gauauaccau uaccauggca cgcaggagcu gauaccggaa     1320

cuccacauug gaauaacaaa gaagcauugg uugaauuuaa agaugcucac gcaaaaagac     1380

aaaccguagu aguacuaggc ucacaagaag gagcggucca uacagcacua gcaggagcac     1440

uagaagcaga aauggacggg gcaaaaggaa gguuaucaag uggacauuua aaguguagac     1500

uaaaaaugga caaauuacga uuaaaaggag uaucauauuc auuauguaca gcagcauuua     1560

cuuuuacuaa gaucccagca gaaacauugc acggaacagu aacuguagaa guucaauaug     1620

caggaacuga uggaccaugc aaaguaccug cucaaauggc uguugacaug caaaccuuaa     1680

cccccguagg acgauuaaua acagcaaauc cgguuauaac agaaucaacu gaaaauagua     1740

aaaugauguu agaacuagau ccaccauuug gugauaguua cauaguuaua ggaguaggag     1800

aaaagaaaau aacacaucau uggcauagga gcggaucaac aauagggaaa gcauuugaag     1860

caacaguaag aggagccaaa agaauggcag uauuaggaga uacggcgugg gauuuuggaa     1920

gcguuggagg cgcauuaaau ucccuaggaa aaggaauaca ucaaauauuc ggagcagcau     1980

uuaaaucauu auuuggugga augucuuggu uuagucaaau acuaauagga acauuauuaa     2040

ugugguuagg auuaaauacu aaaaacggau caauaagccu gaugugcuua gcacuaggug     2100

gaguacuaau cuuuuugagu acagcagugu cagccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10573
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10573
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

uaagcggagu accaguauua ggauuuuuua uaauagcagu uuuaaugucc gcucaagaga      120

gcugggccgu uacuagaaga ggaagugcau auuauaugua ucucgauaga aaugacgcag      180

gagaagcgau aucauuucca acaacauuag gaaugaauaa augcuauaua caaauuaugg      240

aucuaggaca uaugugugau gcaacaauga guuacgaaug uccaauguua gaugaaggcg      300

uagaaccuga ugauguagac uguuggugca acacaacuuc aacaugggua guauacggaa      360

cgugucauca caaaaaaggg gaagcacgaa gaucaagaag ggcaguaacc uuaccaucac      420

acucaacuag aaaauuacaa acuagaaguc aaacauggcu agaaucaaga gaauauacaa      480

aacauuuaau ucgaguagaa aacuggauau ucagaaaucc aggauuugca cuagcagcag      540

cggcaauagc augguuacua ggaucaucaa cuucacaaaa gguaaucuau cuaguuauga      600

uacuucucau agcaccagcu uacaguauua gaugcauugg uguaucuaau cgagauuuug      660

uugaaggaau guccggagga acguggguag acauaguuuu ggaacaugga ggauguguaa      720

cggugauggc acaagacaaa ccaacaguag auauugaauu agugacaaca acaguuucaa      780

auauggcuga agucagaagc uauuguuaug aagcaaguau aagugauaug gccagugauu      840

caagaugccc uacacagggg gaagcauauc uugauaaaca aucugauacu caauaugucu      900

guaaaagaac uuuaguugau agaggauggg gcaauggaug uggcuuauuc ggaaaaggaa      960

guuuaguaac augugcuaaa uucgcauguu caaaaaaaau gacaggaaaa agcauacaac     1020

cugaaaaucu agaauaucgu auaauguuaa guguacacgg aucacaacac ucaggaauga     1080

uaguuaauga uacuggacau gaaacagaug aaaacagagc aaaaguagag auuacaccaa     1140

auaguccgcg cgcugaagca acauugggag gauuuggaag ccuagguuua gacugugaac     1200

caagaaccgg ucuagauuuu ucagaccuau acuauuugac aaugaauaac aaacauuggc     1260

uaguucauaa agaaugguuu caugauauac cacuacccug gcacgcagga gcagauacag     1320

gaacaccuca uuggaauaau aaagaagccu uaguagaauu caaagaugca caugccaaaa     1380

gacaaacagu aguaguauua ggaucacaag aaggagcugu ucauacagca cucgcaggcg     1440

cauuagaagc ugaaauggau ggagcuaaag gacgacuauc uucuggacac uuaaaauguc     1500

gguuaaaaau ggauaaauua cgauuaaaag gaguaucaua uagccuaugu acugcggcau     1560

uuaccuucac aaaaauacca gcagaaacac uacauggaac agucacggua gaaguacagu     1620

augcaggaac agauggacca uguaaaguac cagcucaaau ggcaguugac augcaaacac     1680

uaacaccagu aggaagacua auaacugcaa auccuguaau caccgaaagu acugaaaaca     1740

guaaaaugau gcuugaauua gauccaccgu uuggagauag cuauauagua auaggaguug     1800

gagaaaaaaa aauaacccac cauuggcaca ggaguggauc aacauuagga aaagcuuuuu     1860

cuacaacauu gaaaggagcg caacgucuag cugcauuagg agacacagcu ugggauuuug     1920

gaucaauagg aggaguauuu aauucaauag gaagagccgu gcaccaagua uucggaggag     1980

ccuuuagaac cuuauucggc ggaaugaguu ggauaacaca aggacuaaug ggagcucuau     2040

uacuauggau gggaguuaau gccagagaua gauccauugc acuggccuuu uuagcgacag     2100

gagggguauu aguauuuuua gcaacaaaug uacaugcaug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10574
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10574
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

uaagcggagu accaguauua ggauucuuua uaauagcagu acuaauguca gcucaagaaa      120

guugggccgu aacaagaaga ggauccgcau auuauaugua ucuagauaga aaugaugcug      180

gugaagcaau aucuuucccu accacauuag gaaugaauaa auguuauaua caaauuaugg      240

aucugggaca cacaugugau gcaacgaugu cuuacgagug uccaauguua gacgaaggug      300

uugaaccaga cgauguugau uguuggugca acaccacauc aacuugggua guauauggua      360

caugucauca caaaaaaggc gaagcgagaa gaucaaggag agccguaaca uuaccaaguc      420

acucaacuag gaaauuacaa acaagaucac aaaccugguu agaaucaaga gaauauacaa      480

aacauuuaau aagaguagaa aauuggaucu uucgaaaccc aggauuugcu uuagcugcag      540

cagcaauagc uugguuauua ggaucaagca caucacaaaa aguaauauau uuaguaauga      600

uauuacuaau agcuccagca uacuccauca gauguauugg ugucuccaau agagauuucg      660

uugaaggaau gagcggagga acuuggguag auguaguuuu agaacauggc ggauguguaa      720

cuguaauggc acaagauaaa ccaacaguag acauagaguu aguuacaaca accguaucca      780

auauggcuga aguaagaagc uauugcuaug aagcuucaau aucugauaug gcaagugacu      840

caagaugccc aacacaaggu gaagcauauc uagacaaaca aucagacaca caauauguau      900

guaaaaggac acuaguagac agaggauggg gaaaugguug uggcuuauuu gggaaaggga      960

guuuagucac augcgcaaaa uucgcauguu caaaaaaaau gacaggaaaa ucaauccaac     1020

cagaaaaucu agaauaucgu auaaugcuau cuguucaugg aucucaacau agcggaauga     1080

uuguaaauga cacaggacac gaaaccgaug aaaacagagc aaaaguagaa auaacaccaa     1140

auuccccaag agccgaagca acacuaggag guuuuggauc uuuaggacuc gauugugaac     1200

caagaacagg auuggauuuc agcgaccuuu auuacuuaac aaugaauaau aaacacuggu     1260

uaguacauaa agagugguuc caugauauuc cauuaccuug gcacgcagga gcugauacag     1320

gaacuccaca cuggaauaau aaagaagcau uaguagaauu caaagaugca cacgcaaaac     1380

gacaaacugu aguaguauug ggaucacaag aaggagcagu gcauaccgca uuagcuggag     1440

cacuagaggc agaaauggau ggagcaaaag gccgauuauc aucaggacau cuuaaauguc     1500

gucuaaaaau ggauaaauug agacuaaaag gcguaucaua uucacuuugu accgcugcuu     1560

uuacuuuuac caaaauacca gccgaaacuc uacaugguac cguaacaguc gaaguucaau     1620

augcagggac agauggacca uguaaaguuc cagcacaaau ggcagucgac augcaaacau     1680

uaacgccggu aggaagguug auuacagcaa auccaguaau uacagaaagc acagaaaauu     1740

caaaaaugau guuagaauua gauccacccu uuggagauag uuauauagua auaggaguag     1800

gagaaaaaaa aauuacacac cacuggcacc ggucaggauc uacucuagga aaagcuuuua     1860

guacuacauu aaaaggagca caaagauuag cagcacuagg agauacagcc ugggauuuug     1920

gaucuauagg uggaguguuu aacuccauag gaagagcugu acaccaaguu uuuggcggug     1980

cuuuuagaac ucuuuuuggu gguaugucau ggauaacaca gggacuuaug ggggcuuuac     2040

uacuguggau gggaguaaac gccagagaua gaucuauagc acuagccuuu cuagcaacag     2100

gaggaguauu agucuucuua gcaacuaaug uacacgcaug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10575
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10575
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaaguggagu accuguauug ggauucuuua uaauagcggu auuaauguca gcacaagaau      120

caugggcgau aacaagaagg ggaagcgccu auuauaugua cuuagauaga agcgaugcag      180

gaaaagcaau aaguuuugca acuacucuag gaguaaauaa augccauguu caaauaaugg      240

auuuaggaca caugugugau gcuacaaugu cuuaugaaug uccgauguua gacgaaggag      300

ucgaaccuga cgauguggau ugcuggugua acacaacuuc aacaugggua guauacggaa      360

cuugucauca uaaaaaaggu gaagcgcgaa gaucccgaag agcaguaacu cucccuaguc      420

acucaacacg aaaguuacaa acaagaaguc aaacuugguu agagaguaga gaauauacaa      480

agcacuuaau aaaaguagaa aauuggauuu uuaggaaucc agguuuugcg cuuguagcag      540

uagcaaucgc uugguuauua ggaucaucaa caucacaaaa aguaauauac uuaguaauga      600

uauuacucau agcaccagca uacaguauaa gauguauugg gguaaguaau cgagauuuug      660

uggaaggaau gaguggugga acaugggugg auguaguacu ugagcacgga ggcugcguaa      720

cuguaauggc ucaagauaaa ccaacaguag auaucgaauu aguaacuacu acaguuucaa      780

acauggcuga aguaagaucg uauuguuaug aagcgagcau aucagauaug gccagugacu      840

caagaugccc cacgcaagga gaagcauauc uugauaaaca aagugauacu caauauguau      900

guaaaagaac auuaguagac cgaggauggg gaaauggcug uggauuauuc gguaaaggaa      960

guuuaguaac augcgcaaaa uuuacauguu caaaaaaaau gacaggaaaa agcauucaac     1020

cggaaaaccu agaauaucga auuauguugu cgguucacgg aagucagcau agcggaauga     1080

uaggauauga aacagaugaa gauagagcaa aaguagaagu aacaccaaau aguccacgug     1140

cagaagcaac ucucggcggg uuuggaaguc uaggacuaga uugugaacca agaacaggac     1200

uagauuuuag ugaucuauac uauuuaacaa ugaauaauaa acauugguua guccauaaag     1260

aaugguuuca ugauauacca cuuccauggc augcuggagc agauacaggc acaccacauu     1320

ggaauaauaa agaagcucua guagaguuua aagacgccca ugcaaaaaga cagacagugg     1380

uuguacuagg uagccaagaa ggagcuguuc auacagcauu agcaggagcc cuugaagcag     1440

aaauggaugg agcuaaagga agauuauuua guggacauuu aaaaugcaga cucaaaaugg     1500

auaaacuuag acuaaaaggu guaucuuaca gucuaugcac agccgcauuu acauuuacaa     1560

aagucccagc agagacacua cacggaacag ugacaguaga aguacaauau gcaggcacug     1620

auggaccaug caaaauaccc guacaaaugg caguagauau gcaaacguua accccaguag     1680

gaagacuaau cacugcaaac ccaguaauua cagaaaguac ugagaauuca aaaaugaugc     1740

uagaauuaga uccaccauuc ggagacaguu acauagugau aggaguagga gauaaaaaaa     1800

uuacacauca uuggcauaga ucagguagua cauuaggaaa agcauuuagc acaacauuaa     1860

aaggagcaca aagauuagca gcauuaggcg auacagcaug ggauuuuggg agcauaggug     1920

gcguauucaa cucuauaggc agggccgugc aucaaguauu ugguggagca uucagaacuc     1980

uauuuggagg caugucaugg auuacacaag guuuaauggg agcauuacua uuauggaugg     2040

gaguaaaugc aagagauaga aguauagcau uagcguuuuu agcaacagga ggaguuuuag     2100

uauucuuagc aacuaauguc cacgcaugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10576
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10576
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

uuucaggagu accaguauua ggauucuuua uuauagccgu acuaaugucu gcacaagaaa      120

gcugggcggu gacaagaagg ggaagcgcau acuauaugua ucuagacaga aaugaugcag      180

gagaagcaau aucuuuuccu acaacauuag gaaugaauaa auguuauaua caaauaaugg      240

acuuaggaca uaugugugau gcaacgauga gcuaugaaug cccgaugcuc gacgaaggug      300

uagaaccaga ugauguagau uguuggugua auaccacuag cacaugggua gucuauggaa      360

cgugccauca uaaaaagggc gaagcccgaa gaucuagacg agcaguaacg uugccaucuc      420

auaguacaag aaagcugcaa acaagaaguc aaacauggcu agaaucgaga gaauauacaa      480

aacacuuaau uagaguagaa aauuggauau uuagaaaucc aggauuugcu uuagcagcug      540

cagcaauagc cuggcuauua ggaucaucaa caagucaaaa agugauauau uuaguaauga      600

uacucuugau agcaccagcc uauucaauaa gauguauagg uguaucaaac agggauuuug      660

uggaaggaau gaguggagga acuuggguag acgucgucuu agaacaugga ggauguguaa      720

caguuauggc acaagauaaa ccaacaguag auauagaauu agucacaacu acuguaucua      780

auauggcaga aguaagguca uacugcuaug aagcaucaau uagcgauaug gcuagugaua      840

guagaugccc aacucaagga gaagcauauc uagacaaaca gucagauaca caauauguau      900

gcaaaagaac acuuguagau agaggguggg gaaacggaug uggacuuuuu gggaaaggca      960

gcuuaguaac augugcaaaa uuugcaugca guaagaaaau gacuggaaaa aguauacagc     1020

cagaaaaccu agaauacaga auaauguuaa guguacacgg aucucaacau aguggaauga     1080

uaguaaauga uaccggacac gaaacugaug aaaaucgagc uaaaguagaa auaaccccaa     1140

acaguccgag agcagaagca acuuuaggag gauuuggaag uuuaggacua gacugugaac     1200

caagaacagg acuugacuuu ucugaucucu auuacuuaac aaugaauaau aaacacuggc     1260

uaguacauaa agaaugguuu caugauauac cacuaccaug gcaugccggu gcagauacug     1320

gcacaccuca uuggaauaac aaagaggcuu uaguagaauu uaaagaugca caugcaaaaa     1380

gacaaacagu aguagugcua ggaucacaag aaggugcagu acacaccgca cuagccggag     1440

cauuagaagc ugaaauggau ggugcaaaag gaaggcuauc aucaggacau uuaaaaugua     1500

gguuaaaaau ggauaaacua agauuaaaag gaguaucaua uucauuaugu acugcagcau     1560

ucacauuuac aaaaauaccg gccgaaacgc uacauggaac uguaacaguu gaaguucaau     1620

augcgggaac agauggaccg ugcaaaguac cagcacaaau ggcaguagau augcaaacuc     1680

uaacaccagu uggaagauug auaacagcua auccaguuau aacagaaagu acagaaaaca     1740

guaaaaugau gcuagaauua gauccaccgu uuggagauuc uuacauuguu auaggaguag     1800

gugagaagaa aauuacucau cacuggcaua gaaguggcuc aacacuagga aaagcauuca     1860

gcacuacacu uaaaggagca caaagauuag cagcucuagg ggauacagcc ugggacuucg     1920

gcucaaucgg aggagucuuc aauaguauag ggagagcagu acaccaagua uuuggaggag     1980

cauuuagaac auuauuugga gguaugagcu ggauaacuca agguuuaaug ggagcacuuc     2040

uccuauggau gggugucaac gcaagagacc guaguauagc uuuagcuuuu cuagcaacag     2100

gaggaguauu aguauucuua gcaacaaacg uccaugcgug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10577
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10577
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggacauggau auuauuuuua guagcagcug caacaagagu acauagugua acaaggcgcg      120

gaagcgcuua cuauauguau cuggauagaa acgaugcagg agaagcuaua ucauuuccua      180

ccacacuugg caugaauaaa uguuauaucc aaauaaugga ucuuggucau augugcgacg      240

caaccaugag uuacgaaugc ccaaugcuag augaaggugu agaaccugau gaugucgauu      300

guuggugcaa cacgacuucg acuuggguag uauaugguac augucaucac aaaaaaggag      360

aagcuagaag aaguagaaga gcagucacau uaccaucaca uucaacaagg aaauuacaga      420

caagaaguca aacauggcua gaaucaagag aauauaccaa acauuuaaua agaguugaaa      480

auuggauauu cagaaaucca ggauuugcau uagcagcugc cgccauagca ugguuauuag      540

ggaguucaac aucacaaaaa guaauuuacu uaguuaugau ucuuuuaaua gcaccugcau      600

auagcauuag auguauugga guaaguaaua gagauuuugu ggaaggaaug ucugguggaa      660

caugggugga uaucguacug gaacauggag gauguguaac uguaauggcg caagauaaac      720

caacuguaga cauagaauua guuacaacua caguaucaaa uauggccgaa guuagaaguu      780

auuguuauga agcaucaaua agugauaugg caucagauag uaggugucca acacaaggag      840

aagcuuauuu ggauaaacaa uccgacacac aauacguaug caaaaggacu cuaguugaua      900

gaggaugggg aaauggaugc ggauuauucg guaaaggauc auuaguuacc ugugcuaaau      960

ucgcguguuc uaaaaaaaug acagggaaaa guauucaacc agaaaaccua gaauacagaa     1020

uuaugcuuag ugugcaugga agccaacauu caggaaugau ugucaacgac acaggucaug     1080

aaacagauga aaaucgagca aaaguagaaa uaacaccuaa uucaccacgu gcagaagcaa     1140

cauuaggagg auuuggauca uuaggauuag auugugaacc aaggacagga uuagauuuca     1200

gcgacuuaua cuaucugacc augaauaaua aacacugguu aguacacaaa gaaugguucc     1260

augauauacc auuaccaugg cacgcaggag cagacacagg aacaccacac uggaauaaua     1320

aagaggcccu uguggaauuc aaagaugcac augcaaaacg acaaacagua guaguacuug     1380

gaucacaaga gggagcaguu cauacagcau uagcaggagc acuugaggca gaaauggacg     1440

gugcaaaagg aagauuaagu aguggacauu uaaaauguag acuaaaaaug gacaaacuaa     1500

gauuaaaagg aguuagcuau aguuuaugua cagcagcauu cacauucaca aaaauuccag     1560

cagaaacauu acacggaaca gucacaguag aaguacaaua ugcaggaaca gacggaccau     1620

guaaaguucc ggcacaaaug gcaguagaua ugcaaacauu aacaccaguu ggacguuuaa     1680

uaacagcuaa uccaguaaua acugaaagca cggaaaauuc uaaaaugaug cuagaacucg     1740

auccaccuuu uggagauucu uacaucguaa uaggaguggg ggaaaaaaaa auuacucacc     1800

acuggcauag aaguggauca acauuaggua aagcauucag uacaaccuua aaaggagcac     1860

aacgacuagc agcauuaggc gacacagcau gggauuuugg uucgauagga ggaguguuua     1920

auucaauagg acgcgcaguu caucaaguuu uuggaggagc auuuagaaca uuauuuggag     1980

gaaugucaug gauaacacag ggacuuaugg gagcccuauu auuauggaug ggagugaaug     2040

cuagagauag aucaauagcu cuagcauucu uagcaacagg ugguguauua guguuucuag     2100

caacaaaugu acacgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10578
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10578
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggacuuggau acucuuuuua guagcugcag caacaagagu acacucagua acacgaaggg      120

gauccgcaua uuauauguau uuagauagaa acgaugcagg cgaagcaauu ucauuuccaa      180

caacauuagg caugaauaaa ugcuauauuc aaauaaugga ccuagggcau acaugcgaug      240

ccacuaugag cuaugaaugu ccaauguuag augaaggagu ggaaccagau gauguagacu      300

gcugguguaa uacaacaagu acauggguug uauauggcac augccaucau aaaaaagggg      360

aagcaagaag auccagaaga gcuguaacau uaccuagcca uaguacaaga aaauuacaaa      420

caagaagcca aacauggcua gaaucaagag aguauacaaa acaccuaaua agaguagaaa      480

auuggauauu uagaaaccca gguuucgcau uggcagcagc agcaauugcg ugguuacuag      540

gaucaucaac aucacaaaaa guaauauauc uaguaaugau auuauuaaua gcaccagcuu      600

auucaauaag auguauaggc guaaguaaua gagauuuugu agaaggaaug agcggaggaa      660

caugggugga uguaguauua gagcauggag gauguguaac cguaauggca caagauaaac      720

caacaguuga uauagaguug guuacuacaa cuguuucaaa uauggcagag guacguucuu      780

acugcuauga agcaaguauc agcgauaugg caagugacuc acguugccca acucaggggg      840

aggccuauuu agacaaacaa uccgauacac aauauguuug uaaaagaaca cuaguagaca      900

gaggaugggg aaacggaugu gguuuauuug gaaaaggcag uuuagucacu ugugcaaagu      960

ucgcauguag uaaaaaaaug acagguaaaa gcauccaacc agagaaccua gaauacagaa     1020

uaauguuaag uguucacgga ucucaacaua gcggaaugau ugugaacgau acuggucaug     1080

aaacggauga aaauagagcu aaaguagaaa uuacaccaaa uagucccaga gcagaagcaa     1140

cccuaggcgg auucggauca uugggacuug acugcgaacc uagaaccgga cuugauuuuu     1200

cugacuugua cuacuuaaca augaauaaua aacacugguu aguucauaaa gaaugguucc     1260

augauauacc acugccaugg cacgcaggag cagauacugg uacaccucau uggaauaaua     1320

aagaagcauu aguugaauuc aaagacgcac augcaaaaag acaaacagua guaguauuag     1380

gaagccaaga aggagcagua cacacagcac uagcaggagc uuuggaagca gaaauggacg     1440

gugcuaaagg aagauugagc aguggacauc uaaaaugucg acuuaaaaug gauaaauuaa     1500

gauuaaaagg uguaagcuau ucacuaugua cugcagcauu cacauuuacg aaaauaccag     1560

cagaaacucu acacgguaca guuacaguag aaguccaaua ugcaggaaca gauggaccau     1620

guaaaguucc cgcacaaaug gcaguggaca ugcaaacauu aacuccgguu ggaagauuaa     1680

uaacugcaaa uccagugauu acggaaucga cagaaaauuc aaaaaugaug cuugaauuag     1740

acccuccuuu uggagauuca uacauaguaa ucgguguagg ugaaaaaaaa auuacacauc     1800

auuggcaucg gucagguuca acuuuaggga aagcauuuuc gacaacacua aaaggcgcac     1860

aaagauuagc agcacuaggc gacacagcau gggauuucgg aaguauagga ggaguuuuua     1920

auagcauagg aagagccgua caucaaguau uuggaggagc uuuucggacu cuauuuggag     1980

gcaugucaug gauaacacaa gguuuaaugg gggcauuauu auuauggaug ggaguuaaug     2040

cacgugauag aagcauagcg uuagcauuuu uagcaacugg aggaguauua guauucuuag     2100

caacaaacgu acaugcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10579
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10579
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggacauggau acuauuucua guagccgcag cgacuagagu acauucaauu acaagaagag      120

gaagcgcaua uuauauguau uuagauaggu cagaugcugg gaaagcaaua ucauucgcaa      180

cuacuuuggg uguaaacaaa ugccacguac aaauaaugga ucuaggccau augugugaug      240

caacaauguc auaugaaugu ccaauguuag acgaaggugu agaaccagau gauguugauu      300

gcugguguaa cacaaccuca accugggugg uuuaugguac uugucaccac aagaaaggug      360

aagcuagaag gaguaggagg gccguaacac uuccaucaca uagcacaaga aaacuucaaa      420

cuagaucaca aacaugguua gaaucaaggg aauacacaaa acaucuaauc aaagucgaaa      480

auuggauauu uagaaaccca ggauuugcuc uaguugcagu agccauagcc uggcucuuag      540

gaucaagcac uucacagaaa guuauauauu uaguuaugau acuauuaaua gcacccgcuu      600

auuccauaag auguauagga gugaguaauc gagacuuugu agaaggaaug ucagguggua      660

cgugggugga uguaguauua gaacauggag gauguguaac aguuauggca caagacaaac      720

caacaguaga uauagaauua gucacaacaa caguaucaaa uauggcagaa guuagaucau      780

auugcuacga agcgucaaua agugauaugg ccucagauag uagauguccu acacaaggag      840

aagcguauuu agacaaacaa ucagacacac aauauguuug uaaaaggacu cuaguugaua      900

gaggaugggg uaauggaugu ggauuauucg gaaaaggauc ccuaguaaca ugugccaaau      960

uuacauguag uaagaaaaug accggaaaau cuauacagcc agaaaauuua gaauaucgua     1020

uaauguuaag cguacauggu agucaacaca gcgguaugau aggguaugaa accgacgaag     1080

acagagcaaa aguagaagua acaccaaacu caccaagagc ugaagccacc uuaggaggau     1140

ucggaagucu uggauuagau ugugaaccaa gaacuggauu agauuucuca gaccuuuauu     1200

auuuaacuau gaauaauaaa cauugguuag uacauaaaga augguuucac gacauaccuu     1260

uaccauggca ugcaggagca gauacaggaa cgccacacug gaacaauaaa gaagcauuag     1320

uagaauuuaa agaugcucac gcuaaaagac aaacaguugu aguauuaggg ucacaagaag     1380

gugcuguaca uacugcauua gcaggagcac uagaagccga aauggacgga gcaaaaggca     1440

gauuauuuag uggacaucuu aaaugcagau uaaaaaugga uaaacuaaga cuaaaaggag     1500

uaagcuacag ccuauguacu gccgcauuua cauuuacaaa aguuccagcu gaaacacuac     1560

augggacagu aacaguagaa guccaauacg caggaacuga uggaccaugu aaaauaccag     1620

uacaaauggc aguagacaug caaacacuua caccaguugg gagguuaaua acagcuaauc     1680

ccguaaucac ugaaucaaca gaaaauagua aaaugauguu agaacuugac ccaccauuug     1740

gagauucaua cauuguaauu ggaguaggug auaagaaaau aacacaucau uggcauagau     1800

cuggaucaac acuuggaaaa gcauucagca caacauugaa aggagcacaa agauuagcgg     1860

cauuggggga uacagcaugg gauuucggaa guauaggagg aguauuuaau ucaauugguc     1920

gagcagucca ccaaguauuu ggaggagccu uuagaacauu auucggagga augucaugga     1980

uuacacaagg auuaauggga gcccuauugc uuuggauggg aguuaaugca cgagaccguu     2040

cgauagcucu cgcauuucuc gcuacuggcg gaguucuagu auuuuuagca acgaauguuc     2100

augcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10580
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10580
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggacauggau ucuauuucuu guagcagccg caacaagggu acauaguguc acaagaaggg      120

gaagugcaua uuauauguau cuagacagaa augaugcugg ugaagccaua aguuuuccaa      180

caacgcuagg aaugaauaaa uguuacaucc aaauaaugga ucucggccau augugugaug      240

cgacaaugag uuaugaaugc ccaaugcucg augaaggggu agaaccagau gauguagauu      300

guugguguaa uacaacauca acuuggguag uuuauggaac augucaucau aaaaaaggug      360

aagcuagaag aucgaggaga gcuguaacuu uaccuucuca uagcacaaga aaauuacaaa      420

caagaagcca aacuugguua gaaagcagag aauacacaaa acauuuaauu agaguagaaa      480

auuggauauu ucgaaaucca ggauucgcau uagcugcagc agcaauagca uggcuucuag      540

gcuccaguac aucucaaaaa gugaucuauu uaguaaugau acuucuaauc gcaccagcau      600

acaguaucag augcaucggu guaucaaaua gagauuuugu ugaaggaaug ucgggcggaa      660

cauggguaga cgucguacuc gagcauggcg gcuguguaac aguaauggca caagacaaac      720

caacaguuga uauagaauua guuacaacua cgguaaguaa uauggcagaa guuaggagcu      780

acuguuauga agcaucaaua ucagauaugg caucagauuc aaggugccca acgcaaggag      840

aagcauauuu agauaaacaa agugacacuc aauacguaug uaaaagaaca uuaguugaua      900

gaggaugggg aaacggaugu gguuuauuug gaaaaggaag cuuaguaaca ugugcaaaau      960

uugccuguuc aaaaaaaaug acaggaaaaa guauacaacc agaaaaucua gaauaccgua     1020

uaaugcuauc aguacaugga agucaacaca gcgggaugau aguaaacgac acaggacacg     1080

aaacagauga aaaucgugca aaggucgaaa uuaccccaaa cagcccaaga gcagaagcaa     1140

cucuuggagg uuucggauca uuaggacuag auugcgagcc aagaaccggu uuagauuuua     1200

gcgauuuaua cuauuuaaca augaauaaua aacauugguu aguucauaaa gaaugguuuc     1260

augauauacc acuaccaugg caugcaggug cggauacugg aacaccucau uggaacaaua     1320

aagaagcacu aguagaauuc aaagacgcac augcuaaaag acaaacaguu gucguauuag     1380

gaagucaaga aggagccgua cauacugcac uagcaggugc guuagaagcc gagauggaug     1440

gagcaaaagg ccgacuuagu agcggucauu uaaaaugcag auuaaaaaug gauaaauuga     1500

gacugaaagg ugugucauau ucacuaugua cugcagcauu uacauucaca aaaauaccug     1560

cagaaacacu acacggaacu gucacaguag aaguacaaua cgcagguaca gaugguccau     1620

guaaaguacc agcucaaaug gcuguagaca ugcaaacauu aacaccaguu ggaagacuaa     1680

uaacagcaaa ucccguaaua acugaaagca cagaaaauuc aaaaaugaug uuggaacuug     1740

auccaccauu uggcgauagu uacauaguaa uaggaguugg agaaaaaaaa auaacacauc     1800

auuggcacag aaguggaagc accuuaggaa aagcuuuuuc aacaacauua aaaggagcac     1860

aaagauuagc agcauuaggu gacacagcau gggauuuugg cucaauuggu gguguauuca     1920

auaguauagg gagagcagua caucaaguuu ucggcggagc auuuagaacc uuauuuggcg     1980

gaaugaguug gaucacucaa ggacuaaugg gagcacuauu acucuggaug gguguaaaug     2040

caagagauag aagcauagcc uuagcauuuc uugcuacagg aggaguauua guauuccuag     2100

cuaccaaugu acaugcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10581
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10581
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

aacguagugc aggaucgaua auguggcuag ccucauuagc aguaguaaua gcaugugccg      120

gagcaguaac gaggcgcgga ucagcuuauu auauguaccu agacagaaau gaugcaggag      180

aagcaauauc auucccuaca acacuuggaa ugaauaaaug uuauauacaa auaauggauu      240

uaggacauau gugcgacgca acuaugaguu acgaaugccc aaugcuugau gaaggcguag      300

aaccagauga uguugauugc uggugcaaca caacgucaac cuggguagug uaugggacau      360

gucaucacaa gaaaggagaa gcuagaagaa guagacgagc aguaacguua ccgagucaua      420

guacgagaaa auuacaaaca cgaucacaaa caugguuaga aagcagagag uacacaaaac      480

auuuaaucag agucgaaaau uggauuuuca ggaauccugg guuugcauua gcagcagcgg      540

caauagcaug guuauuagga ucaaguacau cacaaaaagu aaucuaucua guaaugauuc      600

uguuaaucgc ucccgcauau uccauuagau guauaggagu aucaaacaga gauuuugugg      660

aaggaauguc cggaggaaca uggguagaua uaguuuugga acauggaggg uguguaacag      720

ucauggcaca ggauaaacca acgguggaua uagagcuagu aacaacaaca guaucaaaua      780

uggcagaagu aagaucuuau uguuaugaag caucuauaag ugauauggcc agugacagua      840

gauguccaac acaaggggaa gcauaucuag acaaacaaag ugauacacaa uacguaugua      900

aaagaacacu uguagauaga ggauggggaa auggaugcgg auuauucgga aaaggaucuu      960

uaguaacaug cgcaaaauuc gcaugcucaa agaaaaugac agguaaaagu auucaaccag     1020

aaaaucucga auauagaauu auguuaagug uucauggauc acaacacagu gguaugauag     1080

uaaacgauac agggcaugaa accgaugaaa acagagcaaa aguagaaaua acaccuaauu     1140

caccuagagc agaagcuacu cugggaggau ucgggagucu uggacuagau ugcgaaccaa     1200

gaacaggucu ugauuuuucu gaucuauauu aucucacaau gaauaauaaa cauugguuag     1260

uucacaaaga augguuucac gacauaccgu uaccauggca ugccggagca gauacgggaa     1320

cuccacauug gaauaauaaa gaagcucuug uagaauuuaa agaugcucau gcaaaaaggc     1380

aaacaguagu aguuuuaggc agccaagaag gagcaguaca cacagcuuua gcuggagcau     1440

uagaagcaga aauggaugga gcgaaaggaa gacuaucguc aggucauuug aaaugcagac     1500

uuaaaaugga caaauuaaga cuaaaaggag uuucauauuc auuauguacu gcagcauuca     1560

cuuuuacaaa aauaccagca gaaacacuac auggcacagu aacuguagaa guacaauaug     1620

cuggaacaga cggaccaugc aaagucccug cccaaauggc aguggauaug caaacacuaa     1680

caccaguggg aagacuaaua acagcaaauc caguaauaac agaaucaaca gaaaacucaa     1740

aaaugauguu ggaauuagau ccaccuuuug gagacaguua uauuguaaua ggcguaggag     1800

aaaagaaaau uacacaccau uggcauagaa guggaagcac acuuggaaaa gccuuuagca     1860

caacauuaaa aggagcucaa agauuagcug cacuaggaga uacagcgugg gauuuuggaa     1920

guauaggagg ugucuuuaau aguaucggca gagcaguaca ucaaguauuc ggaggagcau     1980

ucagaacacu auuuggcggc augucaugga uuacacaagg uuuaauggga gcucuauuau     2040

uauggauggg uguaaacgcu agggaucgga guauugccuu agcuuuucua gcaacaggag     2100

guguguuagu auuuuuagca acgaacgugc acgcaugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10582
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10582
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aacgaucagc aggaucuaua auguggcucg caucauuagc cguaguuaua gcaugugcug      120

gagcaguaac aagacgaggu agcgcauacu auauguaucu ggauagaaau gaugcgggag      180

aagcuauauc guuuccaaca acacuaggua ugaauaaaug uuauauacaa auaauggauu      240

uaggacauac augugaugca acaaugucau augaaugucc aaugcuagac gaaggaguug      300

aaccagacga cguagacugc ugguguaaua caacaaguac uuggguaguc uauggaacgu      360

gccaucauaa aaaaggugaa gcaagaaggu cccgaagggc aguuaccuua cccagucauu      420

caacuagaaa acuucagaca agaucacaaa ccuggcuaga aucacgagag uauacaaaac      480

auuuaauaag agucgaaaac uggauauuua gaaauccugg auucgcacua gccgcagcug      540

caauagcaug gcuccuagga uccuccacaa gucaaaaagu aauauauuua guaaugaucc      600

uacucauugc accagcauau ucuauaaggu guaucggagu aagcaaucga gacuuuguug      660

agggaaugag uggcgguaca uggguagaug uuguacuaga acacggcggc ugcguaacag      720

uaauggcuca agauaaaccu acaguagaua uagaauuagu aacuacuaca guaucuaaca      780

uggcagaagu aagaucuuac uguuaugaag ccucaauaag cgauauggcc ucagauucaa      840

gauguccaac acaaggagaa gcauacuuag auaaacaauc ugauacacaa uacguaugca      900

agagaacacu aguagauaga ggauggggua augguugugg auuauucgga aaaggaagcc      960

uaguaacuug ugcaaaauuu gcauguagua aaaaaaugac gggaaaaagu auccaaccug     1020

aaaauuuaga auauagaaua augcuaucag uucauggcag ucaacauagc ggaaugauug     1080

uaaaugauac aggucaugaa accgacgaaa acagagcaaa aguagaaauu accccaaaua     1140

guccuagagc agaagccacu uuaggaggcu uuggaucccu aggucuugau ugugaaccaa     1200

gaacaggauu agauuuuucg gacuuauauu accuuacaau gaauaauaaa cacuggcuag     1260

uucauaaaga augguuccau gacauaccuu uaccuuggca ugcaggagca gacacuggca     1320

caccacacug gaacaauaaa gaagcgcucg uagaauucaa agacgcccau gcaaaaagac     1380

aaacuguugu aguauuggga ucacaagaag gcgcuguaca uacagcauua gcaggagcuc     1440

uugaagcaga aauggaugga gcgaaaggaa gacuaucauc ggggcacuua aaaugcagau     1500

uaaaaaugga uaaguuacgu uuaaaagggg uuagcuacuc ucuuugcaca gcagcauuua     1560

ccuuuacaaa aauaccagcc gaaacacuac acgguaccgu aacaguagaa gugcaauaug     1620

cuggaacuga ugguccaugu aaaguaccug cacagauggc uguagauaug caaacacuaa     1680

cgccaguagg aagauuaaua acagcaaauc caguaauaac ugaaucaaca gaaaacucaa     1740

aaaugauguu ggaacuagau ccaccauuug gagauaguua uauaguaaua ggaguaggag     1800

aaaaaaaaau aacucaccac uggcauagau cagguaguac guuagguaaa gcauuuucga     1860

caacauuaaa aggagcacag agauuagcug cacuuggaga uacggcuugg gauuucggau     1920

caauaggagg aguauuuaau aguauuggaa gagcgguaca ucaaguuuuu gguggagcau     1980

uuagaacccu auuuggagga augucuugga uuacacaagg acuuauggga gcauuauuau     2040

uauggauggg aguaaacgcu cgagacagau caaucgcauu agcauuuuua gcgacaggag     2100

gaguacuagu auuucucgca acaaacguac acgcuugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10583
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10583
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaagaagugc aggaucaaua augugguuag caucacuggc gguaguuaua gcaugcgcag      120

gagcuauaac aaggagggga ucagcauacu auauguauuu agacaggagu gaugcaggaa      180

aagcaaucuc auuugcaacc acacuaggug uaaauaaaug ucauguccaa auaauggacc      240

uaggucauau gugugaugcu acaaugucgu augaaugccc aaugcuugac gaaggugugg      300

aaccagauga cguagauugc uggugcaaca caacaagcac auggguaguu uaugggacau      360

gucaccauaa gaaaggugaa gcacgaagau cacggcgagc aguaacuuua ccaucacauu      420

caacaaggaa acuacaaaca agaucacaaa caugguuaga aucaagagag uauacaaaac      480

aucuaauuaa agucgaaaau uggauauuua gaaauccugg auuugcccua guugcaguug      540

caauagcuug gcuucuugga agcucaacau cccaaaaagu aauuuauuua guaaugauac      600

uacugaucgc uccugcauac aguauaagau guauaggagu aaguaauaga gacuucgugg      660

aaggaauguc cggagguacu uggguugaug uaguauuaga acacggugga ugcguaacag      720

uaauggcaca agauaaaccu acaguagaca uagagcuagu aacaacaacu guaucgaaua      780

uggcugaagu uagauccuau uguuaugaag caaguauauc ggauauggca ucagacagua      840

gauguccaac acaaggagaa gcauacuuag auaaacaaag cgauacacaa uauguaugua      900

aaagaacauu gguugauaga ggauggggaa auggaugugg auuauucgga aaaggaaguc      960

uaguaacuug ugcaaaauuc acguguucaa agaaaaugac aggaaaauca auacagccgg     1020

aaaaucuaga auauagaaua augcuaucag uccauggaag ccagcauuca ggaaugauag     1080

gauacgaaac agaugaagau agagcaaaag uagaaguaac uccaaauuca ccaagagcag     1140

aagcaacuuu aggcgguuuu gguuccuuag guuuagacug cgaaccaaga acaggauuag     1200

auuuuucaga uuuguacuac cuaacuauga acaauaaaca uuggcuaguu cauaaagaau     1260

gguuucauga cauuccauua cccuggcaug cuggagcaga cacaggaacu ccacauugga     1320

auaauaaaga agccuuggua gaguuuaaag augcacacgc aaaaaggcag acuguaguag     1380

uauuaggcuc ucaggaaggg gcgguacaca cagcauuagc aggagcauua gaagcagaaa     1440

uggauggagc gaaggguaga uuauuuagug gccauuuaaa guguagauua aaaauggaua     1500

aauuaagauu aaaaggagua ucauauaguc uuuguacagc agcuuucaca uuuacuaaag     1560

ugccagcaga aacauuacau ggaacaguaa caguugaagu acaauaugcu ggaacagaug     1620

gaccauguaa gauaccaguc cagauggcag uagacaugca aacucuaaca ccagucggga     1680

gauuaauuac ugcaaacccg guaauaacag aaagcacaga aaacagcaaa augauguuag     1740

aauuagaccc accauuuggu gacaguuaua uaguaauagg uguaggagac aagaaaauua     1800

cucaucacug gcaucgaagu ggaagcacuc uagguaaagc auucucuaca acacuaaaag     1860

gagcacaaag gcuagcagca cuaggugaca cagcauggga cuuuggaucc auaggaggag     1920

uuuuuaacuc aauagguaga gcugugcauc aaguauucgg aggagcauuu cguacuuuau     1980

ucggaggaau gaguuggauc acacaaggau uaaugggagc acuauuauua uggaugggag     2040

ucaaugcaag agauagauca auagcacuag cauuuuuagc aacuggagga guguuaguau     2100

uucuagcuac uaauguacac gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10584
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10584
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaagaucagc aggaaguaua augugguuag caagucuagc aguaguaaua gcaugcgcag      120

gagcuguaac gcgaagggga ucagcauauu auauguauuu agauagaaau gacgcaggag      180

aagcaauauc auuucccacu acauuaggaa ugaacaaaug uuauauacaa auaauggacu      240

uaggacauau gugugaugca acaaugucau acgaaugucc aauguuagac gaaggcguag      300

aaccagauga uguagauugc uggugcaaua cuacaaguac auggguaguc uacggaacau      360

gucaucauaa gaaaggagaa gccagaagaa gcagacgagc aguuacacua ccaucacaua      420

guacgagaaa auuacaaaca agaucacaaa ccuggcuaga aaguagagaa uauacaaaac      480

auuuaauaag agucgaaaau uggauauuua gaaauccagg auuugcauua gcagcggccg      540

caauagcaug gcuacuuggu ucuucaacau cacaaaaagu aauauauuua guaaugauuu      600

uacuaaucgc accagcauac ucaauaagau guauaggcgu aagcaacaga gauuuuguag      660

aagggaugag uggaggaacu uggguagaug uuguauuaga acacggcggu ugcgucacag      720

uaauggcaca agacaaacca acaguagaua uagaauuggu aacaacaacc guaaguaaua      780

uggcagaagu aagaucauau uguuacgaag caucaauaag ugauauggcu ucagauucaa      840

gauguccuac ucaaggagag gcuuacuuag acaaacaauc agacacucaa uacgucugua      900

aaagaacacu cgucgauaga ggauggggua auggaugcgg acuauuugga aaaggaucau      960

uaguaacaug ugcaaaauuc gcaugcagua agaaaaugac aggaaaaagc auacaaccgg     1020

aaaaucuuga auacagaaua augcuuucag uucauggauc acaacauagu ggaaugauag     1080

ucaaugauac aggucaugaa accgaugaaa auagagcaaa gguagaaaua acaccgaaca     1140

guccaagagc agaagcaaca cucggaggau uuggaucauu agguuuagau ugugaaccaa     1200

gaacgggacu agauuuuagc gauuuauauu accuaacuau gaauaauaaa cauugguuag     1260

uacauaaaga augguuucau gauauaccuu uacccuggca ugcaggagca gauacuggaa     1320

cuccucauug gaauaauaaa gaagcauuag uagaauuuaa agacgcacac gcuaaaaggc     1380

aaacaguugu gguuuuagga agucaggaag gagcaguaca cacagcauua gcgggagcau     1440

uagaagccga aauggacgga gcaaaaggcc gauuaucauc aggacaucuu aaauguagac     1500

uaaaaaugga uaaacuaaga uuaaaaggag uuucuuauuc auuguguaca gcagcauuua     1560

ccuuuacaaa aauaccggcu gaaacuuugc acggaacggu gacaguagaa guacaauaug     1620

cagguacaga cgguccuugu aaaguaccag cccagauggc aguagacaug caaacacuaa     1680

cgccaguagg uagauuaauc acagcaaacc caguaauaac ggaaagcaca gaaaauucua     1740

aaaugauguu agaauuagau ccaccuuucg gagacucaua uauagucaua ggagugggug     1800

agaaaaagau aacacaccau uggcacagaa gcggaaguac auuaggaaaa gcauucucaa     1860

caacacuaaa aggagcacaa cgucuagcug cauuggguga uacugcaugg gauuuuggca     1920

gcauaggugg aguauuuaau agcauuggua gagcugugca ucaaguuuuc ggaggagcau     1980

uccgaacauu auuuggcgga augucaugga uaacacaagg auuaauggga gcacuauuau     2040

uauggauggg aguuaaugca agagauagga guaucgcacu agcauucuua gcaacaggag     2100

gaguauuagu guuuuuagca acuaauguac acgcuugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10585
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10585
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguuggaau aguaggccua cuuuuaacaa cagcaauggc agcaguuaca uuaccaaguc      120

auaguacaag aaaacuacag acaagaagcc aaacaugguu agaauccaga gaguacacua      180

aacauuuaau caggguagaa aauuggauau uuagaaaucc aggauuugca cuagcagcag      240

ccgcaauagc auggcuauua ggaucuucua caucacaaaa aguaauuuau uuaguaauga      300

uacuauuaau agcaccugcu uauaguauaa gguguauugg aguuucaaau cgagacuuug      360

uugaaggaau guccggugga acauggguag acauaguauu agaacauggc gguuguguaa      420

cuguaauggc gcaagauaaa ccaacuguag auauagagcu aguaaccacc acaguaagua      480

auauggcaga aguuagaagu uauuguuaug aagcgucaau aagugacaug gcaagugaua      540

gccgaugucc aacucaaggc gaagcauauu uagacaaaca aucagauacu caauauguuu      600

gcaaaagaac auuaguagau agagguuggg gaaauggcug uggauuauuu gguaaaggau      660

cauuaguaac uugcgcaaaa uuugcauguu caaaaaaaau gacaggaaaa ucuauacaac      720

cagaaaaucu agaauacaga auaaugcuaa guguacacgg aucccaacau ucaggaauga      780

uuguaaauga uacuggacau gaaacagaug aaaauagagc aaaaguagaa auuacaccaa      840

acucaccucg agcagaagca acauuaggcg guuuuggauc uuuaggauua gauugcgaac      900

cacguacagg uuuagauuuc ucagaucucu auuaucucac caugaauaau aaacauuggc      960

uaguacauaa agaaugguuu caugauauuc cauuaccaug gcacgcgggg gcagauacag     1020

gcacuccaca uuggaacaau aaagaagcau uaguagaauu uaaagacgcc caugcuaaaa     1080

gacaaacagu uguaguacua ggaagccaag aaggagccgu acauacagca uuagcugggg     1140

cacuagaagc agaaauggau ggagcaaaag gaagauuaag uucuggucau cuaaaaugua     1200

gauuaaaaau ggacaaacua cgauuaaaag guguuagcua uagcuuaugu acagcagcau     1260

uuacauuuac aaaaauaccu gcagaaacuu uacauggaac aguaacaguc gaaguacaau     1320

acgcaggaac agauggccca uguaaaguuc cagcacaaau ggccguagac augcagacau     1380

uaacacccgu aggaagauua auuacugcaa auccaguaau aacggaaucc acagaaaacu     1440

caaaaaugau guuagagcuu gacccaccgu uuggagauag uuauauagug auaggaguag     1500

gagaaaaaaa aauaacucac cauuggcaca gaagugguuc uacaauagga aaagcauucg     1560

aagcaacggu cagaggugca aaaagaaugg cuguauuagg agacacagca ugggacuuug     1620

gcagcguagg aggagcacua aacucauuag gaaaaggaau acaccaaauu uucggagcgg     1680

cauucaaaag ucuuuuugga ggaaugucuu gguuuucaca aauauuaaua ggaacacuac     1740

uaaugugguu aggacuaaau acaaaaaaug gaucaauauc ccugaugugc cuagccuuag     1800

gaggcguacu aauauuuuua uccacugcag uaucagcuug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10586
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10586
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggaau aguaggauua uuauuaacaa cagcaauggc ugcugucacc cuaccaaguc      120

auaguacaag aaaacuacaa acuagaucac aaacauggcu agaaagucga gaauauacca      180

aacauuuaau aagaguggaa aauuggaucu uuagaaaucc aggauuugcu cuggcugcag      240

cggcaauagc augguuacuu ggaucaagua caucacaaaa aguaauauau uuaguuauga      300

uauuacuuau agcaccagcc uauaguauac guuguauagg cguuagcaau agagauuuug      360

ucgaaggaau gagcggagga acauggguag acguaguacu agaacaugga ggauguguaa      420

ccguaauggc acaagacaaa ccuacggugg auauagaauu aguaacgaca acaguaucua      480

auauggcaga gguacgaagc uacuguuaug aagcaaguau aucagauaug gcaagugaua      540

gcagaugucc aacacaagga gaggcauacc uagauaaaca aagugauaca caauaugugu      600

gcaaaagaac ccuaguagau cgaggcuggg gaaauggcug ugguuuauuu gguaagggau      660

cauuaguaac augugcaaaa uuugcaugcu cuaagaaaau gacaggaaaa ucaauacaac      720

cugaaaaucu ugaauauaga auaauguuga guguacaugg aucucaacau agcggaauga      780

uugugaauga cacaggacac gagacagaug aaaauagagc uaaaguagaa auaacaccaa      840

auucaccaag agcagaagca acauuaggag gauucggauc guuagguuua gauugugaac      900

ccagaaccgg auuagauuuu agcgauuuau auuaccuaac aaugaacaau aaacauuggu      960

uaguacauaa agaaugguuu caugauauuc ccuuaccuug gcaugcugga gcagauacag     1020

gaacaccaca cuggaacaau aaagaagcau uaguagaauu uaaagaugca cacgcaaaaa     1080

gacaaacugu gguaguacua ggaagucaag aaggugcagu acacacagca uuagcaggag     1140

cauuagaagc agaaauggac ggagcuaaag gaagauuauc uucaggacac cuaaagugua     1200

gauuaaaaau ggacaaacug agacuaaaag guguaaguua uaguuuaugu accgcagcuu     1260

uuacauuuac aaaaauacca gcagaaacac uacacggaac aguaacgguc gaagugcaau     1320

augcaggaac agauggacca ugcaaagugc cagcacaaau ggcgguagac augcaaacau     1380

uaacaccagu aggaagauua auuacugcaa auccaguuau aaccgaaagc acagaaaacu     1440

caaaaaugau guuagaacua gauccaccuu uuggagauag uuauauagua auaggaguag     1500

gugaaaagaa aaucacacac cauuggcaua gaaguggcuc aacaauagga aaagcauucg     1560

aagcaacagu aagaggggca aaaagaaugg cuguacuagg agauacagca ugggauuuug     1620

gaucagucgg aggagcauua aacagcuugg gaaaaggaau acaucaaaua uuuggagcug     1680

cauuuaagag uuuauucggc ggaaugaguu gguuuaguca aauauuaaua ggaacuuuac     1740

ucaugugguu aggguuaaau gccaaaaaug gcucgauuag ucuaaugugu uuagcuuuag     1800

gaggaguauu aauauuucua agcacagcgg ucucagcuug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10587
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10587
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

guauaggaau aauaggacua cuauuaacaa cagccauggc agcaguaaca cuaccuucac      120

acucgaccag gaaacuacaa acccgaaguc aaaccugguu agaaaguaga gaauacacua      180

aacaucuaau aaaaguugaa aauuggauau uuagaaaucc aggauuugcu uugguugcag      240

uugcuauagc auggcuauua ggaagcagca caucucaaaa aguuauuuau cuaguuauga      300

uacucuuaau agcuccugca uauaguauac gcuguauagg aguaucaaau agagauuuug      360

uagaaggaau gucaggaggc acauggguag auguuguacu agaacacggu ggauguguaa      420

caguaauggc acaagauaaa ccaacgguag acauagaauu aguaacaaca accguaagua      480

auauggcaga aguacgauca uacugcuaug aagcaucaau aagcgacaug gcaagugauu      540

cuagaugccc aacacaaggu gaagcauauc uagauaaaca aucugacacg caguauguau      600

guaaacgcac gcuaguagau agaggauggg guaacgguug uggucuauuu gguaaaggau      660

cucuuguaac augcgcaaaa uucacauguu cuaagaaaau gacaggaaaa agcauucagc      720

cagaaaaucu agaauacaga auaaugcuau cuguucaugg aucucaacau uccgguauga      780

uaggauauga aacugaugaa gauagagcua aaguagaagu aacaccaaau aguccaagag      840

cugaagcaac acuaggaggc uucgguaguu uaggguuaga uugugaaccg agaacaggac      900

uugacuuuag ugaccuauau uaucuuacaa ugaacaauaa acauugguua guccauaaag      960

aaugguucca cgauauacca cuaccauggc augcuggugc agauacagga acaccacacu     1020

ggaauaauaa agaagcucua guagaguuca aagaugcaca ugcaaaaaga caaacaguag     1080

uaguacuagg aagucaagaa ggggcaguac auacagcauu agcaggagca uuagaagcag     1140

aaauggacgg agcaaaagga cgucucuucu ccggacaccu aaaaugcagg cuaaaaaugg     1200

acaaauuaag guuaaaagga guaucuuauu cacuuuguac agccgcauuc acauuuacaa     1260

aaguaccagc agaaacacua cauggaacug uaacaguuga aguucaauau gcuggaacag     1320

auggaccaug uaaaauaccu guacaaaugg caguagauau gcaaacuuua acaccaguag     1380

guagauuaau aacagcaaac ccuguuauaa cagaaaguac agaaaacagc aaaaugaugc     1440

uagaacuaga cccuccauuc ggagacucuu acauaguaau uggaguagga gacaagaaaa     1500

uaacacauca uuggcaccga ucuggcucaa caauaggaaa agcauuugaa gcaacaguaa     1560

gaggagcaaa aagaauggca guauuaggag auacugccug ggauuuugga ucaguaggag     1620

gaguauucaa uagcuuaggc aaaggcauac aucaaauauu uggugcugca uucaaaaguu     1680

uauuuggggg aaugucaugg uuuucacaaa uucuaauagg aacacuauua guauggcuag     1740

gauuaaacac gaaaaaugga agcauuaguc uaacaugucu agcucuaggu ggaguaauga     1800

uauuccuauc aacagcagua ucagcaugag gacuagugca ucacauuuaa aagcaucuca     1860

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     1920

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     1980

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2100

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2158


<210>  10588
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10588
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

caguaggaau agucggauua uuauuaacaa cagcaauggc cgcaguaaca uuaccaagcc      120

auaguacuag aaaauuacaa acacgaagcc aaacaugguu ggaaucaaga gaauacacaa      180

aacaucuuau aagaguggaa aauuggauau ucagaaaucc aggauuugca cuagcagcag      240

cagcuauagc auggcuucua ggcaguagua caucacaaaa agucauuuau uuaguaauga      300

uauuacuuau agcuccagca uauucuauuc guuguauagg aguaucaaac agagacuuug      360

uugaaggaau gaguggagga acuuggguag auguaguuuu agaacauggu ggauguguaa      420

caguaauggc acaagauaaa ccuacaguag auauagagcu aguuacaaca acgguaucaa      480

auauggccga aguaaggagu uauuguuaug aagcaucaau aucagauaug gcaucagaua      540

guagaugucc aacacagggc gaagccuauc uagauaaaca aucagacaca caauauguau      600

guaaaaggac acugguggau agaggauggg gcaacggaug uggcuuauuu ggaaaaggua      660

guuuagucac augugcaaaa uuugcauguu cuaagaaaau gacagguaaa ucuauccaac      720

cagaaaaucu agaauauaga auaaugcuua gcguucaugg aagucaacac agugguauga      780

uugugaauga uacuggacau gaaacagacg aaaauagagc aaaaguagaa auaacaccaa      840

acucacccag agcugaagca acacuaggag gauucggauc acuuggacua gauugugaac      900

cuagaacugg acuugauuuc agugauuuau acuacuuaac uaugaauaau aaacauuggu      960

uaguacauaa agaaugguuc cacgacauuc cacuaccuug gcaugcuggu gcagauacag     1020

gaacgccaca uuggaauaau aaagaagcac ucguagaguu caaagaugca caugcaaaac     1080

gacaaacggu aguuguauua ggaagucaag aaggcgcagu ccauacagca uuagcuggag     1140

cacuagaagc ugaaauggau ggagcaaaag guagauuaag uuccggacac cuaaaaugua     1200

gacuaaaaau ggauaaauua agacugaaag guguaagcua uagucugugc accgcugcau     1260

uuacauucac aaaaauaccu gcugaaacau uacauggaac aguaacaguu gaaguacaau     1320

augcaggaac agacggaccu ugcaaaguac ccgcacagau ggcuguagac augcaaacau     1380

uaaccccagu gggaagacua auaacagcaa auccaguaau aacagaaagc acugaaaauu     1440

caaaaaugau guuagaacuc gauccaccau uuggggauag cuauauagua auaggaguag     1500

gcgaaaagaa aauaacacac cacuggcaua gaagugguag uaccauugga aaagcauuug     1560

aagcuacagu cagaggagca aaaagaaugg caguacuugg cgauacagcu ugggauuuug     1620

guucuguagg cggagcuuua aacaguuuag gaaaaggaau acaucaaauc uuuggagcag     1680

ccuuuaaaag uuuauuugga ggaaugaguu gguuuuccca aauuuuaaua ggaaccuuau     1740

uaauguggcu agguuuaaau acgaaaaaug ggagcaucag cuuaaugugu cuagcacuug     1800

gugggguauu aauauuucua agcacagcgg uaucugcaug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10589
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10589
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaagcggagu accaguauua ggauucuuca uaaucgcagu acuaaugagu gcacaagaaa      120

guugggcagc agugacauua ccaagucaca guacaagaaa auuacaaaca cguagucaaa      180

caugguuaga aagucgagaa uauacaaaac aucuaauacg uguagaaaac uggauauuua      240

gaaauccagg auuugcuuug gcagcagcug ccauagcaug gcuucuagga agcucaacau      300

cacaaaaagu uauauaucua gucaugauuc uucuaaucgc cccugcauau ucaauaagau      360

guauaggagu cucaaacaga gauuucguag aaggaauguc aggaggaaca uggguagaca      420

uaguccuuga acacggagga uguguaacug uuauggccca agauaaaccu acuguagaua      480

uagaauuggu cacaacuaca guaagcaaca uggcagaagu aagaucauau uguuaugaag      540

cuagcauauc agauauggca ucagauucaa gaugcccaac acaaggugaa gcauaucuag      600

auaaacaauc agauacacaa uacguaugua aacgaacauu aguagauaga ggauggggaa      660

auggaugugg acuuuuugga aaaggaucau uaguaacaug cgccaaauuu gcguguucaa      720

agaaaaugac uggaaaauca auacaaccgg aaaaccuaga auauagaaua auguuaagug      780

uucauggauc acaacauuca gguaugauag uuaaugauac aggacaugaa acagaugaaa      840

acagagcaaa aguagaaaua acaccaaauu caccaagagc agaagcaaca uuaggcgguu      900

ucggaaguuu aggacuugau ugugaaccua gaacagguuu agauuuuuca gaccuauauu      960

auuugacaau gaauaacaaa cacugguuag uacacaaaga augguuccau gauauaccac     1020

uaccauggca ugcuggugcg gauacaggaa cuccccauug gaauaauaaa gaagcguuag     1080

uagaauuuaa agacgcacac gcaaaaagac aaacuguagu aguauuggga ucucaagaag     1140

gagcaguaca cacugcauua gcaggagcac uagaagcaga aauggaugga gcuaaaggca     1200

gacuuagcag uggacauuua aaaugucguu uaaaaaugga caaauuaaga uuaaaagggg     1260

uauccuacuc ccuaugcaca gcugcuuuua cuuucacaaa aauuccugca gaaacacuuc     1320

auggaaccgu aacaguagaa guacaauacg cgggaacaga ugggcccugc aaaguacccg     1380

cacaaauggc aguagauaug caaacauuaa caccuguagg cagauugaua acugcuaauc     1440

caguaauaac agaaucaaca gaaaauagua aaaugaugcu agaauuagac ccaccauuug     1500

gcgauaguua uauagucauu ggaguugggg agaagaaaau aacucaucac uggcacagau     1560

caggaaguac uauaggaaaa gcauucgaag caacagugag aggagcaaaa agaauggcag     1620

uauuagguga uacagcuugg gauuuuggau caguaggagg agcacuaaac ucucuaggua     1680

aaggaaucca ucaaauuuuu ggugcagccu uuaaaucauu auuuggagga augucauggu     1740

ucagucaaau auuaauaggu acauuauuaa uguggcuggg acuaaauacu aaaaacggua     1800

guauaagccu aaugugucua gcacucggug guguacuaau auucuuaagu acagcagucu     1860

cagcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10590
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10590
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcga       60

uaaguggagu accaguguua ggauuuuuua uaauagcagu acuaaugucu gcucaagaau      120

caugggcagc agucacauua ccaucacacu caacuaggaa auuacagacc cgaucgcaaa      180

ccuggcugga aucaagagaa uauacaaaac acuuaauaag aguagaaaau uggauauuua      240

gaaaucccgg auucgcacua gcagcggcug caauugcuug guuacuugga uccaguacau      300

cccaaaaagu aauauauuua guaaugauac uucuuaucgc accagccuau aguauuagau      360

guauuggagu aucaaauaga gauuuuguag aaggaauguc aggagguacu uggguugaug      420

ugguacuaga acacggcgga ugugucacag uaauggcaca agauaaaccc acaguagaua      480

uagaauuagu aacgacaaca gugucaaaua uggcagaagu aagaucauau ugcuacgaag      540

caaguauaag ugacauggca ucagauucac gauguccaac acaaggagaa gcauauuuag      600

auaaacaauc agacacacaa uauguaugua aaagaacauu agucgauagg gguuggggaa      660

acggaugcgg cuuauuugga aaaggauccu uaguaaccug ugcaaaauuc gcauguucga      720

aaaagaugac aggaaaaucu auacaaccag aaaauuuaga auauagaaua augcuaucag      780

uacacggauc ucaacauuca gguaugauag uaaaugauac uggacaugaa acagacgaga      840

acagagcaaa aguagaaaua acuccaaaua gcccacgugc ggaggccaca cuaggaggau      900

uuggaagccu ggggcuagac ugugaaccua gaacaggacu agauuucuca gaccuauauu      960

auuuaacaau gaauaacaaa cauugguuag uccacaaaga augguuucau gauaucccac     1020

uaccauggca cgcaggugca gauacgggua caccacauug gaauaacaaa gaagcauuag     1080

ucgaauuuaa agaugcccau gcaaaaagac aaacaguagu aguacuaggu ucacaagaag     1140

gagcugugca uacagcacua gcuggugcau uagaagcaga aauggaugga gcaaaaggaa     1200

gauuaucaag cggacauuug aaaugucgac uaaaaaugga uaaacuuagg cuuaaaggug     1260

uaagcuacuc uuuauguacc gcagcauuca cguucacaaa aauaccagcc gaaacauuac     1320

acgguacugu aacaguagaa guacaauacg cagguacaga uggaccaugu aaaguaccag     1380

cucaaauggc aguagacaug caaacuuuaa caccaguagg gagauuaaua acagcgaauc     1440

caguaauuac agaauccaca gaaaacagua aaaugaugcu agaauuagau ccacccuuug     1500

gagacucaua uaucguaaua gguguaggug aaaaaaaaau aacacaucac uggcaucgau     1560

cuggaucaac cauaggaaaa gcauuugaag cuacaguaag aggagcgaaa aggauggcag     1620

uuuuaggaga cacugcaugg gauuuuggua guguaggagg agcauuaaau ucauugggua     1680

aaggcauaca ucaaauauuc ggagcagcau uuaaaagcuu auuuggagga augaguuggu     1740

uuucacaaau acuaauagga acuuuacuua ugugguuagg ucuaaaugcu aaaaauggcu     1800

caauaucacu aauguguuua gcacuaggug gaguacuaau auuuuuaagu acagcuguau     1860

cagcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10591
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10591
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

uaucaggugu accagucuua gguuucuuua uaauagcagu auuaauguca gcacaagaaa      120

gcugggccgc aguaacucua cccagucacu cgacaaggaa acuacaaacc agaucacaaa      180

caugguuaga aaguagagaa uauacaaaac accuuauuaa aguagaaaau uggauauuua      240

gaaauccugg guucgcguua gucgcaguag caauagcaug gcuauuagga agcagcacaa      300

gucaaaaagu aauauaccua guaaugauau uacuaaucgc gccagcauau aguauacguu      360

guauaggagu uucaaacaga gacuuuguag aaggaauguc aggagguaca uggguagaug      420

ucguauuaga acauggagga ugugucacag uuauggcuca agauaaacca acaguagaua      480

ucgaauuagu aacaacuacu gucaguaaua uggccgaggu uagaucauac uguuacgaag      540

caucaauauc agauauggca agugauagua gaugcccaac acaaggagaa gcauauuuag      600

acaaacaauc agauacucaa uacguaugua aacgaacacu aguagacaga gguuggggaa      660

acggaugugg auuauuugga aaaggaaguu uaguaacaug ugcaaaauuu acuugcucaa      720

aaaaaaugac uggaaaauca auucaaccgg aaaaucuaga auaucgaaua augcuaagug      780

uacauggaag ccaacauuca ggaaugauug gcuaugaaac ugaugaagac agagcuaaag      840

uugaagugac accaaacuca ccuagagcag aagcaacauu aggagguuuc gguucauuag      900

gauuagauug cgaaccacga acagguuuag auuucaguga ucuauauuau uuaacaauga      960

acaauaaaca uuggcuagua cauaaagaau gguuccauga uauaccacuc ccauggcaug     1020

cgggugcaga cacaggcacu ccacacugga acaauaaaga agcuuuagua gaauuuaaag     1080

augcacacgc aaaaagacag acaguaguug uacuaggauc acaagaagga gcaguacaca     1140

ccgcacuugc aggcgcauua gaagcggaaa uggacggagc caaagguaga uuauuuagug     1200

gacauuuaaa auguagauua aaaauggaua aauuaagacu aaaaggagua agcuacucau     1260

uaugcacagc agccuuuaca uuuacaaaag uacccgcuga aacauugcac ggaacaguua     1320

caguugaagu ucaauacgca ggaacagaug gaccauguaa aauaccagua caaauggcag     1380

ucgacaugca aacauuaaca ccaguagguc gacuaauaac agcaaacccc guuauaaccg     1440

aaucuacuga aaacucgaaa augaugcuag aacuagaccc accguuugga gauucauaua     1500

uaguaauagg cguaggagau aaaaaaauaa cacaucauug gcacagaucc ggaagcacaa     1560

uaggaaaagc auuugaagcu acaguaagag gagcuaaaag aauggcagua uuaggagaua     1620

cagcauggga cuuuggaagu guaggaggug uauuuaacag uuuaggaaaa ggaauacauc     1680

aaauauuugg cgcagccuuu aaaagccuau ucggaggaau gagcugguuu ucacaaauuc     1740

uaaucggaac ucuacuagug uggcuaggac uuaauacaaa aaaugguagc auaaguuuaa     1800

caugucuagc auuaggaggu guuaugauau uucuaucaac agcgguaagu gcaugaggac     1860

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     1920

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     1980

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2040

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2160

ggcucuuuuc agagccacca gaauu                                           2185


<210>  10592
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10592
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

uuucuggagu uccaguauua gguuuuuuua uuauagcggu acuaaugagu gcacaagaaa      120

gcugggcagc aguaacauua ccuagucaua guacuagaaa acuacaaaca agaucacaaa      180

caugguuaga aucgcgagaa uauaccaaac auuuaauaag aguagagaac uggauuuuca      240

gaaauccagg cuucgcccua gcagcagcag caauagcaug guuauuagga aguucaacau      300

cacaaaaagu uauauaucua gucaugauau uauugauagc gcccgcauau ucaauacgau      360

guaucggagu aucaaauaga gauuuuguag aaggaauguc uggaggaacu uggguagacg      420

ucguacuaga acacggggga uguguaacag uaauggcuca agauaaaccu acaguagaua      480

uagaauuagu aacgacaaca guaagcaaca uggcagaagu cagauccuau uguuaugaag      540

ccaguauuag cgacauggcg agcgauagca gauguccaac acaaggggaa gcuuauuuag      600

auaaacaauc agacacacaa uacguaugua aaagaacauu aguagauaga gguuggggaa      660

augguugugg acuuuuugga aaaggaaguu uaguaacaug ugcaaaauuu gcaugcucua      720

aaaaaaugac aggcaaauca auacaaccag agaaccucga auauagaaua auguuaagcg      780

uacauggaag ucaacauagc gguaugauag uaaaugacac cggacacgaa acagacgaaa      840

auagagcaaa aguagaaaua acgccaaaua guccgcgcgc agaagcaaca uuaggaggau      900

ucggaucacu aggauuagac ugugaaccaa gaacaggacu ugauuuuucu gaucuauacu      960

aucuuacaau gaauaauaaa cauugguuag uucauaaaga augguuccau gauauuccuu     1020

uaccauggca cgcaggugca gauacaggaa caccacauug gaauaauaaa gaagcauuag     1080

uagaauucaa agaugcucau gcuaaacguc aaacuguagu uguauuagga agccaagaag     1140

gagcaguuca uacugcauua gcaggagcau uagaagcuga aauggauggu gcaaaaggua     1200

gauugaguag uggacauuua aaauguagac uaaaaaugga caaauuacga cuaaaaggag     1260

uuucauauag ucuauguacu gcugcauuua cauucacaaa aauaccagcu gaaacauuac     1320

acggaaccgu uacaguugaa guacaauacg caggcacaga uggaccaugc aaaguuccag     1380

cacaaauggc aguagacaug caaacauuaa caccaguagg aagacuaauu acagcaaauc     1440

cuguaauaac agaaucaacu gaaaauagca aaaugauguu agaauuagau ccaccauuug     1500

gagauucaua uauugugaua ggaguaggag aaaaaaaaau uacacaccac uggcauagau     1560

cugggucaac aauagguaaa gcauucgaag cuacuguaag aggagcaaaa agaauggcag     1620

uacuuggaga uaccgccugg gauuucggua guguaggagg agcacuaaac ucacuuggaa     1680

aaggcauaca ucaaauauuu ggagcagcau uuaaaucacu auuuggugga augucauggu     1740

uuucacagau cuuaauaggu acacuacuaa uguggcuagg acuuaauaca aaaaauggau     1800

caauaucauu aauguguuua gcauuaggag gaguuuuaau uuuuuuaagc acagcaguaa     1860

gugcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10593
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10593
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggacauggau auuauucuua guugcagcug caacaagagu acauagugca guaacauugc      120

cgucacacuc aacaaggaaa uuacaaacaa gaagccaaac augguuagaa agccgagaau      180

acacaaaaca uuuaauuaga guagaaaacu ggauauuuag aaauccagga uuugcucuag      240

cggcagcagc aauagcaugg uuacuuggau caucaacaag ccagaaagua auauacuuag      300

uaaugauacu ucuuauagcu ccagcauacu caauacgaug uauuggagua agcaauagag      360

auuuugucga aggaauguca ggaggaacau gggucgacau cguauuagaa caugguggau      420

guguuacagu aauggcacaa gauaaaccua cuguagauau agaacucgua acuacaacug      480

uaucaaacau ggcagaagua agaucauacu gcuaugaagc aucaauaagu gauauggcau      540

cggauaguag auguccuaca caaggagaag cauauuuaga uaaacaauca gauacacaau      600

acguauguaa acguacuuua guagauagag guuggggaaa uggaugcgga uuauuuggaa      660

aagguaguuu aguaacaugu gcaaaauuug cuuguucaaa gaaaaugaca gguaaaagua      720

uacaaccaga aaauuuggaa uauagaauaa uguuaucugu acauggaagc caacauagug      780

gaaugauugu aaaugauaca ggacaugaga ccgaugaaaa uagagcaaaa guagaaauaa      840

cuccaaauuc accacgagca gaagcgacau uaggaggauu cgguucauua gguuuagauu      900

gugaaccaag aacagggcua gauuuuucug aucuauauua ucuaacuaug aacaauaaac      960

auuggcuagu acauaaagag ugguuucaug acauaccauu accauggcau gcuggagcag     1020

auacaggaac accacauugg aacaauaaag aggcuuuggu ugaauuuaaa gacgcccacg     1080

caaaaagaca gacaguaguc guuuuaggca gucaagaagg agcaguucac acagcauuag     1140

cuggagcauu agaagccgaa auggacggag caaaaggucg auuaucaagu ggacaccuaa     1200

aauguagauu aaaaauggac aaacuacgac uuaaaggggu uucauauuca cuauguacag     1260

cagccuuuac cuuuacaaaa auaccagcag aaacuuuaca ugguacagua acagucgaag     1320

uccaauaugc agguacagau ggcccaugua aagucccggc acaaauggca guagauaugc     1380

agacacuaac cccaguagga agacuuauaa cagcaaaucc aguuauaaca gaaucuacug     1440

aaaauucaaa aaugauguua gaguuagauc cuccauuugg agauagcuau aucguaauag     1500

gagucggaga aaagaaaaua acacaucauu ggcauagaag uggaaguacc auaggaaaag     1560

cauuugaagc aacaguaaga ggagccaaaa gaauggcagu acuuggagau acggcauggg     1620

auuuuggcag cguaggaggu gcauugaaua guuuaggaaa aggaauacau caaaucuuug     1680

gugccgcauu caaauccuua uuuggaggaa ugagcugguu uucacaaaua cuaaucggaa     1740

cauuacuaau gugguuagga uuaaauacua aaaauggaag cauaaguuua augugccugg     1800

cacuaggagg aguacuaaua uuuuuaagua cagcagugag cgcuugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10594
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10594
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggacauggau auuauucuua guagcugcag caacccgagu acacucagca gucacauuac      120

ccagucauag cacaaggaaa uuacaaacca gaucacaaac uugguuagaa ucaagagaau      180

acacaaaaca ucucauaaga guugaaaacu ggauauuuag aaaccccggc uuugcucuag      240

cagcagcagc aauugcaugg cuacuaggaa gcucuacauc acaaaaggua auauauuuag      300

uaaugauacu auuaauagca ccagcuuaca guauuagaug uauaggaguu ucgaauagag      360

acuuuguaga aggaauguca ggagguacau ggguagaugu aguacuagaa cauggcggau      420

gugugacagu uauggcacaa gauaaaccaa ccguagauau agaauuagug acgacaacag      480

uaucaaauau ggccgaagua agaucuuauu guuaugaagc auccauuucu gacauggccu      540

cagauaguag augccccacu caaggagaag cauacuuaga uaaacaguca gacacucaau      600

acguuuguaa aagaacucua guagauagag guugggguaa uggaugugga uuauuuggaa      660

aaggaagccu aguaacaugc gcaaaauuug cauguaguaa aaagaugacu ggaaaaucaa      720

uacaaccaga aaaccuugaa uaucgaauua uguugucagu ccacggaagu caacauagug      780

gaaugauagu aaaugacaca ggacacgaaa cagacgaaaa uagagcuaaa guagaaauaa      840

caccaaauuc uccgagagcu gaagcaacac uuggaggauu ugguagcuua gguuuagacu      900

gugaaccaag gacuggauug gacuuuucag aucuauauua uuuaacaaug aauaauaaac      960

acuggcuagu acacaaagaa ugguuucacg auauuccauu accauggcau gcaggagcug     1020

acacagguac accacauugg aauaauaaag aagcauuagu agaguucaaa gaugcacacg     1080

cgaaaagaca aacaguagua gugcuagguu cacaagaagg agcaguacau accgcauuag     1140

caggagcauu agaagcagaa auggauggag caaaagggag acuaucaagu ggacauuuaa     1200

aauguagauu gaagauggau aaauuaagau ugaaaggggu aucuuacagu uuauguacug     1260

cagcuuucac auuuacaaaa auaccagcag aaacacuaca uggaacagua acaguugaag     1320

uacaauacgc aggaacagau ggacccugca aaguuccagc ucaaauggca guagauaugc     1380

aaacucuaac gccaguagga cgguuaauaa cagccaaucc agugauuaca gaaucgacag     1440

aaaacucaaa aaugaugcuc gaauuagauc cacccuuugg agauucauau auuguuauag     1500

gaguaggaga aaagaaaaua acacaucauu ggcauaggag cggcucaacu auaggaaaag     1560

cauucgaagc aacaguaaga ggagcuaaac gaauggcagu auuaggagau acagcuuggg     1620

acuucggaag cguuggagga gcccuaaaua guuuaggaaa aggaauacau caaauauucg     1680

gagcagcauu uaaaucauua uuuggaggaa ugaguugguu cucacaaauu cuuauuggaa     1740

cccuauuaau guggcuaggc uugaaugcua aaaauggaag uauaagucuc augugcuuag     1800

cacuaggagg gguauuaaua uuuuuaucca ccgcaguuuc agcaugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10595
<211>  2164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10595
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggacauggau auuauuuuua gucgcagcag caacuagagu acauucugca guuacuuuac      120

caagucauuc aacaaggaaa cuacaaacaa gaucacaaac auggcuagaa ucaagagaau      180

acacuaaaca ucuaauaaaa guagaaaauu ggauauuuag aaaucccggc uuugcacucg      240

uggcaguagc aauagcaugg uuguuaggga gcucuaccag ucaaaaaguu auuuaucuug      300

uaaugauacu acuaauagca ccagcauaua gcauaagaug uauaggaguu agcaacagag      360

acuuugugga aggaauguca ggaggaacau ggguagaugu cguacuagaa cauggcggau      420

guguaacagu aauggcacaa gauaaaccaa caguagauau agaacuagua acaacaacug      480

uaucuaauau ggcagaagua agaucauacu guuaugaagc aagcauuucu gauauggcau      540

cagauagcag augcccaaca caaggagaag cauaucuaga uaaacaaagu gauacacaau      600

acguaugcaa aagaacauua guugacagag gaugggggaa ugguugcggu cuguuuggaa      660

aaggaaguuu aguuacaugu gcuaaauuua caugcaguaa aaaaaugacu ggaaaaagca      720

uucaaccaga aaauuuagaa uaccgaauaa uguuaagcgu acauggaagc caacacucag      780

gaaugauagg auaugaaaca gaugaagaua gagcuaaagu agaagucaca ccgaauucac      840

caagagcuga agcaacucua ggagguuuug guucacuugg uuuagauugu gaaccaagaa      900

caggacuaga cuucucagau uuguauuacc uaacaaugaa uaauaaacau uggcuaguuc      960

auaaagaaug guuucacgac auaccauugc cuuggcacgc aggagccgau acuggaacac     1020

cacauuggaa uaauaaagaa gcacucguag aauuuaaaga ugcacaugcc aaaagacaaa     1080

cuguagucgu acuaggaagu caagaagggg ccguacauac ugcauuagca ggcgcacuag     1140

aagcugaaau ggauggcgca aaagguagac uauucagcgg acaccuaaaa ugucgauuaa     1200

aaauggauaa auuaagauua aagggaguaa gcuauucuuu auguacagcc gcuuuuacau     1260

ucacuaaagu accagcagaa acauuacacg guacaguaac cguagaaguc caauacgcag     1320

gaacagaugg accaugcaaa auuccaguac aaauggcagu agauaugcaa acguuaacac     1380

caguaggucg auuaauuaca gcaaauccag uaaucacaga aucaacagaa aauucgaaaa     1440

ugaugcucga acuagacccu ccauucggag auaguuauau aguaaucgga guaggagaua     1500

aaaaaauaac acaccacugg cauagaagug gcucaacaau agggaaagca uuugaagcaa     1560

caguaagagg agcaaaaaga auggcaguuu uaggugacac ugcaugggac uuugguagug     1620

uaggaggugu auuuaauagc uuaggaaaag gaauccauca aauauuugga gcagcauuca     1680

aaucauuauu cggugguaug ucaugguucu cacaaauucu aauagguaca cuucuuguuu     1740

gguuaggucu aaauacaaaa aauggaucaa uuucucuaac auguuuagcu uuaggagggg     1800

ucaugaucuu cuuaucuacg gcaguaucag cuugaggacu agugcaucac auuuaaaagc     1860

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     1920

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     1980

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2160

aauu                                                                  2164


<210>  10596
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10596
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggacauggau ucuauuucuc guagcagcag caacaagagu ucauucagca guaacauuac      120

caucacauuc aacaagaaaa cuacaaacaa gaagucaaac auggcuagaa ucgagagaau      180

auacaaagca uuuaaucaga guagaaaacu ggauauuuag aaacccugga uuugcauugg      240

ccgcugcugc aauagcaugg uuacuuggaa gcagcacaag ucaaaaagua auauaucuag      300

uaaugauacu auuaauagcc ccagcauauu caauuagaug uauaggaguc ucaaauaggg      360

auuucguaga aggaaugagu ggaggaacau ggguagacgu aguacuagaa cacggugggu      420

gcguaacagu aauggcacaa gacaaaccca caguagauau agaauuagug acaacuacag      480

uuaguaacau ggcagaaguu agaaguuauu guuacgaagc aagcauaagu gauauggcaa      540

gcgauucucg auguccgacu cagggagaag cauaucuaga caaacaaucu gauacacaau      600

auguguguaa acgaacucua gucgauagag gguggggaaa uggaugugga cucuuuggaa      660

aaggaagucu aguaacgugc gcuaaauuug ccuguaguaa gaaaaugacg ggaaaaucca      720

uacaacccga aaacuuagaa uauagaauaa uguuaagugu gcauggaagu caacauagug      780

gaaugauagu aaacgauaca ggacacgaaa cagaugaaaa uagagcaaaa guagaaauaa      840

cacccaauag ccccagagca gaagcaacac uaggagguuu uggaucauua ggacucgauu      900

gugaaccuag aacuggguua gauuuuucag auuuauauua uuuaacaaug aauaauaaac      960

auugguuagu ucauaaagaa ugguuucaug auauaccuuu accauggcau gcuggggcag     1020

acacaggaac accacauugg aauaacaaag aagcauuagu agaauuuaaa gacgcacaug     1080

cuaaaagaca aacuguggua gugcuaggga gucaagaagg ggcaguacau acagcauuag     1140

ccggagcuuu agaagcagaa auggauggag caaaaggaag auuauccagc ggacaucuaa     1200

aauguagauu aaaaauggau aaauuacguu uaaaaggugu gagcuacuca cuuuguacag     1260

cugcauuuac auucacaaaa auuccagcag aaacccuaca cggaacaguc acagucgaag     1320

uacaauacgc aggaacagac ggaccuugca aaguacccgc ucaaauggca gucgauaugc     1380

aaacauuaac accuguaggu agacuaauua cagcaaaucc aguaauaaca gaaucaacag     1440

aaaauaguaa aaugaugcuc gaauuagauc caccauuugg ugauucauau auaguuauag     1500

gaguaggaga gaaaaagauu acacaucauu ggcauagaag uggaaguaca auaggaaaag     1560

cauuugaagc aacaguaaga ggagcaaaac gaauggcagu auuaggagau acagcuuggg     1620

acuuuggaag uguuggaggu gcacuaaaua guuuaggaaa aggaauacau caaauauuug     1680

gagcagcauu uaaaucauua uuugguggua ugucgugguu uucccaaauc uuaauaggaa     1740

cuuuauuaau guggcuagga uuaaauacga aaaauggauc aauaucacuc augugucuag     1800

cauuaggagg aguuuuaauc uuuuuaucaa ccgcaguuuc agcaugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10597
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10597
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaagguccgc aggaaguaua augugguuag cuagcuuagc aguagugaua gcaugugcag      120

gagcugcagu aacauuacca ucacauucca caagaaaacu acaaacaaga ucucaaacau      180

gguuagaauc aagagaauac acaaaacacc uaauacgagu agaaaacugg auauuuagaa      240

auccuggauu cgcacuagcc gcagcagcaa uugccugguu auuaggaagu aguacaagcc      300

aaaaaguaau auauuuagua augauauuau uaauugcacc agcauauagu auaagaugua      360

uaggaguaag uaauagagau uuuguagaag gaaugagugg agguacaugg guagauauag      420

uauuagaaca cggaggaugu guaacaguua uggcucaaga uaaaccuaca guugauauug      480

aauuaguaac cacuacagua ucaaauaugg cugaaguaag aaguuauugu uaugaagcaa      540

guauuagcga uauggcgagu gauagcagau gucccacaca aggugaagca uacuuagaua      600

aacaaaguga cacacaauau guaugcaaaa gaacucuagu agauagagga uggggaaacg      660

guugcggauu auuuggcaaa gguagcuuag ucacaugugc aaaauuugcc ugcaguaaga      720

aaaugacagg aaaaaguaua caaccagaaa auuuagaaua uagaauaaug cuuagcgugc      780

acggaucaca acauucagga augauaguga augauacagg acaugaaaca gacgaaaaua      840

gggcaaaagu ugaaauuacg ccaaauaguc ccagggcaga agcaaccuug ggaggauuug      900

gcaguuuagg acuagauugu gaaccaagaa ccggauuaga uuucucugau cuauauuauu      960

uaacgaugaa uaauaaacac ugguuaguac acaaagaaug guuucaugau auaccauuac     1020

cguggcacgc aggagcagau acaggaacac cucauuggaa uaauaaagaa gcucuaguag     1080

aauuuaaaga cgcccacgcg aaaagacaaa cagucguagu auuagguagc caggaaggag     1140

ccguucacac agcauuagcc ggugcuuuag aagcagaaau ggauggagca aaagguagac     1200

uaagcagugg acacuuaaaa uguagguuaa aaauggacaa auuaagacua aaagguguau     1260

cauauaguuu auguacagcg gcauuuacgu ucacaaaaau ucccgcagaa acauuacacg     1320

gaacaguaac aguagaagua caauaugcag gaaccgaugg accauguaaa guaccagcac     1380

aaauggcagu agauaugcaa acauuaacac cuguaggaag acuuaucaca gcaaauccug     1440

uuauaacaga aagcacagaa aauucaaaaa ugauguuaga auuagaccca ccauucggug     1500

auaguuacau aguaauagga guaggcgaga agaaaauuac acaucauugg cauagaucag     1560

gauccacaau agguaaagcg uucgaagcaa cagugagagg agcaaaaaga auggcaguau     1620

uaggggauac ugccugggau uuuggaucag uagggggagc auuaaauagu cuaggaaaag     1680

guauacacca aauuuuugga gcggcuuuua aaucucuauu uggugggaug ucaugguuuu     1740

cacaaauacu aauaggcaca cuacucaugu ggcuaggauu aaacacgaaa aauggaagca     1800

uaucauuaau gugucuagca cuaggaggug uccuaauauu ccugucaaca gcaguaagug     1860

caugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10598
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10598
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaaggagcgc aggaucaauc augugguuag caucauuagc aguaguaauu gccugcgcug      120

gugcagcagu aacauugcca agucauucua caagaaaacu acagacaaga agccaaacau      180

ggcuagaauc uagagaauac acaaaacauu uaauaagagu ugaaaacugg auauuuagaa      240

auccaggauu ugcacuagca gcagcagcaa uagccugguu auuaggaagu ucaacaaguc      300

aaaaaguaau uuacuuagua augauacuau uaauagcucc agcauauagu auaagaugua      360

uaggaguauc aaauagagau uuuguugaag guaugagugg aggaacaugg guagauguug      420

uacuagaaca uggaggaugu guaacuguaa uggcacaaga uaaaccaaca gucgacauug      480

aacuaguaac aacuacagug ucuaacaugg cugaaguaag aucauauugc uaugaagcau      540

cuauaaguga uauggcauca gauagcagau guccuacuca aggagaagca uaucuugaua      600

aacaaucaga uacacaauau guauguaaaa gaacauuagu agauagagga uggggaaaug      660

guuguggcuu auuuggaaaa ggaagucuag uaacaugugc aaaauuugca ugcaguaaaa      720

aaaugacggg aaaaaguaua cagccagaaa accuagaaua cagaauuaug uuaagcguuc      780

augguaguca acauagugga augauaguaa acgauacugg gcaugaaacu gacgagaaua      840

gagccaaagu agaaauaaca ccaaauucac cgagagcaga agcuacacuu gguggauucg      900

gaucacuugg ucuagauugc gaaccaagaa caggacuuga cuucucagac cuauauuauc      960

uaacaaugaa uaauaaacau ugguuaguuc auaaagaaug guuucaugau auaccauuac     1020

cauggcaugc aggagcagau acgggaacac cacauuggaa uaauaaagaa gcacuagucg     1080

aauuuaaaga ugcacaugca aaaagacaaa cgguaguugu acuaggauca caagaaggag     1140

caguacauac agcauuagcu ggggcuuugg aagcagaaau ggauggcgcg aaaggaagac     1200

uaucaagugg acauuuaaaa ugccgauuga aaauggauaa auuaagauua aaaggaguaa     1260

guuacucauu auguaccgcu gcguuuacau uuacgaaaau uccagcagaa acguuacaug     1320

gaacaguaac agucgaaguu caauaugcag gaacagacgg accauguaaa gugccagcac     1380

aaauggcagu agacaugcaa acauuaacac cagugggaag auuaauaaca gcaaacccag     1440

uaauaacaga aucuacggaa aauagcaaaa ugauguuaga acuagaccca cccuucggag     1500

auucauauau uguaauagga guuggagaaa aaaaaauuac gcaucacugg cauagaagug     1560

guaguacaau uggaaaagca uuugaagcua caguaagggg agcaaaaaga auggcaguac     1620

uaggagauac ugcaugggau uucgguagug uuggaggugc acuaaauagc cuuggaaaag     1680

gaauucauca gauauuugga gcagcauuua aaucauuauu uggaggcaug aguugguuca     1740

gucaaauauu aauuggaacu cuauuaaugu gguuagguuu aaacgcaaag aauggaucaa     1800

uaaguuuaau guguuuagca cuaggaggag uauuaauauu ccuuucaaca gccguuucgg     1860

cuugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10599
<211>  2182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10599
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaagaucagc aggaaguaua augugguuag caucacuagc aguaguaaua gcaugugcug      120

gagcagcagu aacauuacca ucacauucaa caagaaaauu acagacaaga agccaaaccu      180

gguuagaauc aagagaauac acaaaacauu uaauaaaagu cgaaaacugg auauuuagaa      240

auccaggauu cgcucuagua gcaguagcaa uagcuugguu acuaggauca agcacaucac      300

aaaaagugau cuaucuaguu augauacuau uaauagcccc ugcauauucu auaaggugca      360

uaggaguaag caacagagau uuugucgaag gaaugucagg aggaacaugg guagauguug      420

ucuuagaaca uggagguugc guuacaguaa uggcacaaga uaaaccaacc guggauauug      480

agcuaguaac gacaacagua aguaacaugg cagaaguaag aaguuauugu uacgaagcaa      540

guauaucaga cauggcauca gauucaagau guccgacuca aggagaagca uaucuagaua      600

agcaaucaga uacacaauac guuugcaaaa gaacauuagu agauagagga uggggaaaug      660

gaugcggauu auuuggaaaa ggaaguuuag uaacaugcgc aaaauuuacu uguaguaaga      720

aaaugacagg aaaaaguauu caaccugaaa aucucgaaua uagaaucaug cuaaguguuc      780

auggaagcca acauucuggc augauaggau augaaacaga ugaagauagg gcaaaaguag      840

aaguuacacc uaauucacca agagcagaag caacauuagg agguuuugga aguuugggac      900

uagacuguga accucgaacu gguuuagacu uuagugauuu auacuauuua acgaugaaua      960

auaaacauug gcuuguacau aaagaauggu uucaugacau accauuacca uggcacgcag     1020

gagcagauac aggaacacca cacuggaaua auaaagaagc auuaguagag uuuaaagaug     1080

cucaugcaaa acgacaaaca gugguaguau uaggaucuca agaaggagcu guacauacag     1140

cucuggcggg ugcauuggaa gcagaaaugg auggagcaaa aggacgacug uucagcggac     1200

auuuaaaaug caggcuuaaa auggauaaau uaagauuaaa aggaguauca uauucacuau     1260

guacagcugc uuuuacauuc acaaaagucc cagcagagac ucuacaugga acaguaacug     1320

uugaaguaca auaugccgga acagauggac cauguaaaau uccuguacaa auggcaguag     1380

auaugcaaac acuuacucca guaggaagau uaauaacggc aaaccccguu auaaccgaau     1440

caacagaaaa uaguaaaaug augcuagaac uagauccucc auuuggugau ucauauauag     1500

uaauaggagu aggagacaag aaaauaacac accauuggca uagaagugga aguacuauag     1560

gaaaagcauu ugaagcaaca gugagaggag ccaaaagaau ggcuguacua ggagauacag     1620

caugggauuu cgguucagua ggcggaguuu uuaacucauu aggaaaaggg auacaucaaa     1680

uauuuggagc agccuucaaa ucacuguucg gcggaaugag cugguuuucu caaauauuga     1740

uugggacccu auugguaugg cucggacuaa auacuaaaaa ugguagcaua ucauuaacau     1800

gccuagccuu aggaggagua augauuuucc uuagcacagc aguuagcgca ugaggacuag     1860

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     1920

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     1980

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2040

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2160

ucuuuucaga gccaccagaa uu                                              2182


<210>  10600
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10600
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

aaagaucagc aggaagcauc augugguuag cuaguuuagc agucguaaua gccugugcag      120

gagcagcagu aacauuaccg agccacucaa caaggaaauu gcaaacccgg agccaaacau      180

ggcuagaaag uagagaauau acaaaacauc uaaucagagu cgaaaacugg auauucagaa      240

auccuggauu ugcauuagca gcagcagcaa uagcauggcu auuaggauca aguacaaguc      300

aaaaaguaau auaucuaguu augauauuac uaauagcacc agccuacuca auaagaugua      360

uaggaguaag caauagagac uuugucgaag gaaugagcgg aggaacuugg guagauguag      420

uauuagaaca cggagguugc guaacgguca uggcacaaga caaaccaaca guagacauug      480

aauuaguaac aacgacaguu ucaaauaugg cagaaguccg aaguuacugu uaugaagcgu      540

caauaucuga cauggcaagu gacucuagau guccuacuca aggagaggcu uauuuagaua      600

aacaaaguga uacacaauau guauguaaac gaacguuagu ggauagagga ugggguaaug      660

guugcggucu guucgguaaa ggaucucuag uaacaugugc aaaauuugcu uguuccaaga      720

aaaugacagg aaaaucaaua caaccagaaa auuuagaaua ucgaauaaug uuaaguguac      780

auggaaguca acauagugga augauuguaa augauacagg acaugaaaca gaugaaaaua      840

gagcaaaagu ggaaauaacu ccaaauucac caagagcaga agcuacauua ggaggcuuug      900

guagcuuagg auuagacugc gaaccaagga cgggucuaga cuuuagugac cuuuauuauc      960

uaacaaugaa uaauaaacau ugguuaguac auaaagaaug guuccaugau auuccauuac     1020

cauggcacgc aggagcagau acuggaacac cacauuggaa caauaaagaa gcauugguug     1080

aauuuaaaga ugcacaugcc aaaagacaaa caguaguagu acuuggaucc caagaaggag     1140

caguacauac agcauuagcc ggugcauuag aagcagaaau ggacggagca aaagguagac     1200

uuagcagcgg acaucuuaaa uguagacuaa aaauggauaa auuaagauua aaaggaguau     1260

cuuauagucu augcacagca gcauucacuu uuacuaaaau acccgcagaa acguuacaug     1320

gaacaguaac aguagaagua caauaugcag gaacagaugg cccauguaaa guaccagcac     1380

aaauggcagu ugauaugcag acauuaacac cuguaggaag acuaauaaca gcaaauccag     1440

uaauaacaga aucaacagaa aauaguaaaa ugauguugga auuagaucca ccauucggag     1500

auucuuacau aguaauagga guaggagaaa agaaaauaac ccaucauugg cauagaucag     1560

gaaguacaau uggaaaagca uuugaagcaa caguaagagg agcaaaacga auggcaguac     1620

uuggagacac agcaugggau uuugguucgg uaggaggagc auuaaauuca cuagguaaag     1680

gaauacauca aauauuugga gcagcguuua aaucacucuu cgguggaaug ucaugguuca     1740

gucaaauucu aauaggaaca uuauuaaugu ggcucggucu aaauacgaaa aauggaucaa     1800

uuucacucau guguuuagcc uuagguggag uccucauauu cuuaucaaca gcagucagcg     1860

caugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10601
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10601
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaagcggcgu acccguccuc ggauucuuca uaaucgcagu ccucaugagc gcccaagaaa      120

guugggccgu cacccgcaga ggaagugcau acuacaugua ccucgaccgc aacgacgcgg      180

gagaagccau cuccuuccca accacccuag gcaugaacaa augcuacauc caaaucaugg      240

accuaggaca uaugugcgac gccacaauga gcuacgaaug ccccaugcuc gacgaaggug      300

uagaacccga cgacguagac ugcuggugca acacaacauc aaccugggua guauacggaa      360

caugccauca caaaaaggga gaagccagac gcagccgcag agccguaaca cuacccucac      420

acucgacacg aaaacuucaa acacggucac agaccuggcu agaaucccgu gaauacacca      480

aacaccuuau aagaguagag aacuggaucu uccgaaaccc cggguucgcc cucgccgccg      540

ccgccaucgc auggcuacuc ggcuccagca caucacaaaa aguuauauac cuaguuauga      600

uccuccugau agccccugcc uauagcauaa gauguauagg cguaucaaac cgcgacuucg      660

uagaaggcau gucaggcgga acaugggugg acauaguccu agaacacggc ggcugcguca      720

ccguaauggc ccaagacaaa ccaaccguag acauagaacu uguaaccacc acggugucaa      780

acauggccga aguacggagc uacugcuacg aagccucaau uuccgacaug gcgagcgacu      840

ccaggugccc gacacaggga gaagcauacc ucgacaagca aucagacacc caguacgucu      900

gcaagagaac acucguagac agaggcuggg gaaacgguug cggacuguuc ggcaaaggca      960

gccuggucac augcgcgaaa uucgccugua gcaaaaagau gaccggaaag ucgauccaac     1020

ccgaaaaccu agaauacagg aucaugcuga gcguccaugg cagccaacac agcggcauga     1080

ucguaaacga caccggacac gaaaccgacg aaaaccgggc uaaaguagaa aucaccccaa     1140

acucccccag agccgaagcc acgcuagggg gauucggcuc acuugggcuc gacugcgaac     1200

ccagaaccgg acuagauuuc agcgaucuau acuacuuaac caugaacaac aaacacuggc     1260

ucguacacaa agagugguuc cacgacauac cccugccaug gcacgccggc gccgacaccg     1320

gcacaccaca cuggaacaac aaagaagcgc uuguagaguu caaggacgca cacgcuaaac     1380

gccaaaccgu aguagugcug gggagccaag aaggcgcggu acacacggcc cucgccgggg     1440

cacuagaagc agaaauggac ggagccaagg ggcggcuguc aagcggccac cugaaaugcc     1500

gccucaaaau ggacaaacuu aggcuuaaag gagucuccua uagccuaugc acggccgccu     1560

ucaccuucac aaaaauacca gccgaaaccc uccacggcac uguaacaguc gagguccaau     1620

acgccggcac agacggaccc uguaaaguac cagcgcagau ggccguagac augcaaacgc     1680

uuacacccgu aggacgccua aucacagcaa acccaguaau caccgagucc acagaaaaca     1740

gcaaaaugau gcuugaacuc gaucccccau ucggggacuc cuacauaguc aucggcgucg     1800

gcgaaaagaa aauaacccac cacuggcacc gcuccggcuc aacgauagga aaggcauucg     1860

aagcaacagu aagaggagca aaaagaaugg caguccucgg ggacacagcc ugggacuucg     1920

gaucgguagg cggcgcccuc aacagccucg gaaagggcau acaccaaauc uucggagcag     1980

ccuucaaauc acuauucgga ggcaugagcu gguucaguca aauccugauc ggcacccucc     2040

ucauguggcu cggccucaac acgaaaaacg gauccaucuc ccucaugugc cuagcacucg     2100

gcggaguccu cauauuccuu uccaccgcgg ugagcgcgug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10602
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10602
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

uaucgggagu acccguacug ggauucuuca uaauagcagu ccucaugucc gcacaagaau      120

ccugggcagu cacaagacgc ggauccgccu acuacaugua ccuagaccga aacgacgccg      180

gcgaagccau cucauuccca acaacacuag gaaugaacaa augcuacauc caaaucaugg      240

accucggaca uacgugcgac gcaacaauga gcuacgaaug ccccaugcug gacgaaggag      300

uagaaccaga cgacgucgac ugcuggugca acacaaccag cacuugggua guauacggca      360

caugccacca caagaaaggc gaagccagac gaagcagaag ggcaguaacc cuaccuucgc      420

acucgacccg aaaacugcag acaagaagcc agacauggcu ggaaucacgc gaauacacaa      480

aacaccucau acgagucgag aacuggaucu ucaggaaccc cggauuugcc cuggcggccg      540

ccgcaaucgc auggcuccuc ggcagcucaa cgucacaaaa aguaauauac cuggucauga      600

uccuacucau cgcacccgcc uacagcauca gaugcauagg agucagcaac cgggacuucg      660

uagaaggcau gagcggcggc accuggguag augugguucu agaacacggc gguugcguca      720

cagugauggc acaagacaaa cccaccguag auauagagcu agucaccacc accgucagca      780

auauggccga aguccggagc uacugcuacg aagccagcau cagcgacaug gcauccgaca      840

gccgcugccc aacccaagga gaggcauacc uagacaaaca aagugacaca caauacgucu      900

gcaaacgaac acucguagac agaggcuggg gcaacggaug uggccucuuc gguaaaggcu      960

cacucgucac augcgcaaaa uucgcaugca gcaagaaaau gaccggcaaa agcauccaac     1020

cagaaaaccu ugaguauaga aucaugcuau cgguacacgg aucccaacau ucaggcauga     1080

ucguaaacga cacgggccau gaaacagacg aaaaucgagc caaaguagaa auaaccccga     1140

acagcccgag ggcagaggca acacucggug gauuuggcag ccugggccuc gacugcgaac     1200

cccgaaccgg ucuugacuuc uccgaccugu acuaccuaac aaugaacaac aaacacuggc     1260

ucgugcauaa agagugguuc cacgacaucc cacugcccug gcacgccggc gcagacaccg     1320

guacgccaca cuggaacaac aaagaagcac ucgucgaguu caaagacgca cacgcaaaac     1380

gccaaacggu aguaguccuc ggcucacaag aaggagccgu gcacaccgcc cuagccggcg     1440

cacucgaagc agaaauggac ggcgcaaaag gccgacucuc cucaggccac cucaaaugcc     1500

gccuaaaaau ggacaaacug cgccucaaag gagucagcua cuccuuaugc acagcagccu     1560

ucaccuucac aaaaauaccu gcagaaaccc uucacggcac cgucaccguc gaaguacaau     1620

acgcagguac cgacggcccc ugcaaaguac cagcccaaau ggccguagac augcaaaccc     1680

ugacccccgu gggcagacuu auaaccgcca accccguaau aacagaaagc accgaaaacu     1740

caaaaaugau gcucgaacuc gaucccccgu ucggcgacag cuauauaguc aucggagucg     1800

gcgaaaagaa aaucacacau cacuggcaca gaucaggaag caccaucgga aaagccuucg     1860

aggcgacagu acgcggagcc aaacggaugg ccguccucgg agacaccgcc ugggacuucg     1920

gaucaguagg aggggcacuu aacucccuag gaaaaggcau ccaccaaauc uucggcgcag     1980

ccuucaaauc acuauucggg gguauguccu gguucuccca aauccuaauc ggaacucucc     2040

ucauguggcu cggacucaac gcgaaaaacg gcaguaucag ccucaugugc cucgcacuag     2100

gaggagugcu aauauuccua ucaacagcag ucagcgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10603
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10603
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucucaggcgu cccagugcuc ggauuuuuca ucauagcagu auuaaugagc gcgcaagaaa      120

guugggcaau aaccagacgc ggcuccgccu acuacaugua ccuggaccgc uccgacgcag      180

gcaaagccau cagcuucgca accacccucg gaguaaauaa gugccacgug caaauaaugg      240

accuaggcca caugugcgac gccacaaugu cauacgaaug cccgaugcuc gacgaaggag      300

uagaacccga cgacguggac ugcuggugca acacaacguc cacaugggua guauacggaa      360

ccugccacca caagaaagga gaggcaaggc gaagccgcag agcaguaacc cuacccagcc      420

acaguacacg aaaacuacaa acgcggagcc aaacuuggcu ggaaucacgc gaauacacaa      480

aacaccuaau aaaaguggaa aacuggauau uccggaaccc cggauucgca cugguggcag      540

uagcgauagc cuggcugcuc ggcagcucaa ccucccaaaa agucaucuac cucguaauga      600

uacuccugau agcaccagcg uacuccauaa ggugcauagg cgucagcaac cgugauuucg      660

ucgagggaau gucaggagga accuggguag acguaguccu agaacacggg ggcugcguga      720

ccgucauggc ccaggacaag ccuacagucg acauagaacu cgucacaacc acgguaagca      780

auauggcaga aguaagaagc uacuguuacg aagcaucaau cagcgacaug gccagcgacu      840

caagaugccc aacccaagga gaagccuacc uagacaaaca gagcgacacc caauacgucu      900

gcaagcggac gcucguagac agaggcuggg gaaauggaug cggccucuuc ggaaagggaa      960

gccuagugac cugcgccaaa uucacaugca gcaagaaaau gacaggaaaa uccauccaac     1020

ccgagaaccu cgaauaccgc auaauguuaa gcguccacgg gucacaacau agcgguauga     1080

ucggcuacga aaccgacgaa gacagggcaa aaguagaagu aacccccaac agcccaagag     1140

cugaagccac acucggagga uucggcagcc uaggacuaga cugcgaacca cgcacuggac     1200

uugacuucuc cgaccuauac uaccucacga ugaacaacaa gcacuggcua guccacaaag     1260

aaugguucca cgacauaccc cuaccuuggc acgccggcgc ggacaccgga acaccacacu     1320

ggaacaacaa agaggcacuc gucgaguuca aggaugcaca ugccaaacgc caaacagucg     1380

uaguacuggg aagccaggaa ggagccguac acacagcccu cgcaggagca cucgaagcug     1440

aaauggacgg cgcaaaaggc cggcuuuuca gcggccaccu caaaugccgg cuaaaaaugg     1500

acaaacuccg gcugaaaggg guaagcuaca gccucugcac cgcagcguuu acguucacca     1560

aaguacccgc agaaacacua cacggcacag uaacagucga ggugcaauac gcaggcaccg     1620

acggccccug caagauacca guacaaaugg cagucgacau gcaaacacug acaccugucg     1680

ggcgacuaau aaccgcaaac ccagucauca ccgaaagcac cgagaacagc aaaaugaugc     1740

ucgagcuaga cccaccauuc ggcgacagcu acaucgucau aggcgugggc gacaagaaaa     1800

ucacacacca cuggcacagg uccggcagca ccaucggcaa agccuucgaa gcgacaguac     1860

gcggagcaaa acgaauggca gucuugggcg acacagccug ggacuucggc agcgucggcg     1920

gagucuucaa cucacuaggg aaaggaauac accagauauu cggagccgcc uucaagagcc     1980

uauucggagg aaugagcugg uuuagccaga uacucauagg cacccuacuc gucuggcuag     2040

gucuaaacac aaagaacggc uccaucucau uaacaugccu ggcauugggc ggagugauga     2100

uauuucucag cacagccgua agcgcgugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10604
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10604
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uauccggcgu accaguccuc ggguucuuca uuauagcagu ccucaugagc gcacaagaau      120

cgugggccgu aaccagacga ggaucugcau auuacaugua ccuagaccgg aacgacgcgg      180

gagaagccau cucauuuccc acaacacucg gaaugaauaa augcuacauc caaaucaugg      240

accucggcca caugugcgac gcaacgauga gcuacgaaug ccccaugcua gacgaggggg      300

ucgaacccga cgacgucgac ugcuggugca acacaacauc aaccugggua guauacggca      360

caugccacca caaaaagggc gaagcccgac gauccaggcg agcaguaaca uuacccuccc      420

auuccacccg caaacuccaa acgcgcucgc aaaccuggcu agaaucacga gaauacacaa      480

aacaccucau ccgaguagaa aacuggaucu ucagaaaucc gggauucgcc cuggccgcug      540

ccgccaucgc cuggcuacuc ggcuccagca ccucacagaa agucaucuac cucguuauga      600

uacuccucau agcgccagcc uacucuaucc gcugcaucgg aguaagcaac cgagauuucg      660

uagaaggcau gucaggcggc acaugggucg acgucguccu agaacacgga ggcugcguaa      720

cagucauggc ccaagacaaa ccgaccgucg acaucgagcu aguaaccaca accgucucca      780

auauggccga aguccgaagc uacugcuacg aagcgucaau cuccgacaug gcaagcgacu      840

cccgaugccc aacccaaggc gaagccuacc uagacaaaca guccgacacc caauacguuu      900

gcaaacgcac acucgucgac cgaggauggg gcaacggcug cggccucuuc gggaaaggaa      960

gccucguaac cugcgcaaaa uucgcaugca gcaagaaaau gaccggaaaa uccauccaac     1020

ccgaaaaccu ggaauaccgc auaaugcucu caguacacgg aucccagcac agcggaauga     1080

uagugaacga uacaggacac gaaacugacg aaaaccgagc aaaaguagaa aucaccccca     1140

acucaccacg agcagaagcc acccuaggcg gauucggcuc auuaggccuc gacugcgaac     1200

cuaggacggg ccucgacuuc uccgaccuau acuaccugac aaugaacaac aaacacuggc     1260

uaguacacaa agaaugguuu cacgauaucc cccuacccug gcacgccggc gccgacacag     1320

gcacaccgca cuggaacaac aaggaagccu uaguagaguu caaagacgcc cacgccaagc     1380

gccaaaccgu cgugguccug ggaucccaag aaggcgccgu acacaccgcc cuagcaggcg     1440

cccuugaggc cgaaauggac ggggcaaaag gccgccuauc auccggacau cucaagugcc     1500

ggcucaaaau ggacaaacua cgacuaaaag gcguaagcua cucccucugc acagccgcuu     1560

ucacauucac caaaauacca gcggaaacuc uccacggcac cgucaccguc gaagugcaau     1620

acgccggaac agacggacca ugcaaagugc ccgcccaaau ggccgucgac augcagacuc     1680

uaaccccagu agggcgacuc auaaccgcaa aucccgucau caccgaaagc accgagaaca     1740

gcaaaaugau gcucgaacug gacccucccu ucggcgacag cuacaucguc aucggaguag     1800

gcgaaaagaa aaucacccac cacuggcacc gaucgggaag caccaucggc aaagccuucg     1860

aagcgacagu cagaggcgca aaacgcaugg ccguccucgg agacaccgca ugggacuucg     1920

gcagcgucgg aggcgcacuc aacucucugg gcaaagggau ccaccaaaua uucggcgccg     1980

ccuucaaauc ccucuucggg ggaaugucgu gguucucaca gauacuuaua ggaacacuac     2040

ucauguggcu cggacuaaac accaaaaacg gcuccauauc ccucaugugc cuagcccuag     2100

gaggaguccu aaucuuccuc ucgacagcgg ucuccgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10605
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10605
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggacauggau acucuuccua guagccgccg ccacccgcgu acacucagug acgcgccgcg      120

gcucagcaua cuacauguac cucgaccgga acgacgccgg agaggcgaua ucauucccca      180

ccacacuagg aaugaacaaa ugcuacauac agaucaugga ccucggccac augugcgacg      240

ccacaauguc cuacgagugc ccaaugcugg acgaaggggu agaaccagac gacgucgacu      300

gcuggugcaa caccacgucc acaugggugg ucuacggcac augccaccac aagaaaggag      360

aagcgcgccg aucacgcaga gccgucaccc ucccaagcca cuccacaaga aaacuccaaa      420

ccagaagcca aacuuggcuc gaaucccggg aauacaccaa gcaccucaua cgcguagaga      480

acuggauauu ccgcaaccca ggcuucgcgc uagcagccgc cgccauagcc uggcuacuag      540

gcucauccac aucacaaaaa gugaucuauc uaguaaugau acuacuaaua gcacccgccu      600

acagcaucag augcauaggc gucucaaaca gagacuucgu agaaggcaug ucaggaggaa      660

ccuggguaga uaucguacuc gaacacggcg gauguguaac cgucauggcc caagacaaac      720

ccacuguaga uaucgagcua guaacaacaa caguauccaa cauggccgaa guaagauccu      780

acugcuacga agcaucaauc ucagacaugg ccucagacuc gaggugcccu acucagggag      840

aagccuaccu agacaaacaa agcgacacgc aauacgucug caaaagaacc cuagucgaua      900

gaggcugggg aaacgggugc gggcuauucg gcaagggauc acucguaacc ugcgcaaagu      960

ucgcaugcuc caagaaaaug accgguaaaa gcauccaacc cgaaaaccuc gaauacagaa     1020

ucaugcuauc gguacacgga ucccagcacu ccggaaugau uguuaacgac acgggucacg     1080

aaacagacga aaaccgggcc aaggucgaaa uaacaccaaa cagcccacga gcagaagcca     1140

cccuaggagg cuucggcucc cucggccucg acugcgaacc ccguaccggc uuggacuuca     1200

gcgaccuaua cuaccucacc augaacaaca aacacuggcu gguccacaaa gaaugguucc     1260

acgacauucc ccuccccugg cacgcagggg ccgacacagg aaccccacac uggaacaaca     1320

aagaggcccu uguagaguuc aaagacgcac acgcaaaacg ccaaacaguc gucguccucg     1380

gcucgcaaga aggagccgua cacacagcac uagccggcgc acuagaagca gaaauggacg     1440

gcgcaaaagg ccgccuaagc ucaggccacc ucaagugccg gcucaagaug gacaaacuac     1500

gccuaaaagg agucucauac ucccuaugca ccgcggccuu caccuucacc aaaaucccgg     1560

cagagacccu acacggaacc guaaccguug aaguucaaua ugccggaacc gacggacccu     1620

gcaaaguacc cgcccagaug gcagucgaca ugcaaacacu uaccccaguu ggccgccuca     1680

ucacggcaaa ucccgucauc accgaaagua cagaaaacuc aaaaaugaug cuagaacuag     1740

acccgcccuu uggcgacuca uacauaguca uagggguagg agagaaaaag auaacccacc     1800

acuggcaccg cagcgggucc acgauaggua aagccuucga agcaaccgua agaggcgcga     1860

aacgaauggc aguacuaggu gacaccgcau gggacuucgg gagcgucggc ggggcacuaa     1920

auucacuugg gaagggaauc caccaaaucu ucggggccgc auucaagagu cucuucggcg     1980

gcaugagcug guucucacaa auccucauag gaacccuccu aauguggcua ggccucaaca     2040

ccaaaaacgg aagcauaucc cuaaugugcc uagcacucgg aggcguacuc aucuuccuca     2100

gcacagcggu aagcgcguga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10606
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10606
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggacguggau acuauuccuc guagccgccg caacccgcgu ccacagcgua acccgaagag      120

gcuccgcuua cuacauguac cucgaccgaa acgacgccgg cgaagccaua ucauucccca      180

ccacccucgg caugaacaaa ugcuacaucc aaauuaugga ccuagggcac acuugcgacg      240

ccacaaugag cuacgagugc cccaugcucg acgaaggcgu cgaaccagac gacguggacu      300

gcuggugcaa caccaccagc acauggguag uauacggaac cugccaccac aagaaaggcg      360

aagcccgaag aucacgccgg gccguuaccc uccccucaca cuccacacga aagcuccaaa      420

cccgcuccca aaccuggcuc gaaucucgag aauacacaaa gcaccuuaua agaguugaga      480

acuggaucuu cagaaacccc ggcuucgccc uagccgcagc cgcaaucgcc uggcuccucg      540

gcuccucaac aucccaaaaa gucauauacc uugucaugau ccuccucaua gcaccagccu      600

acucgauccg augcaucggc guuuccaacc gcgacuuugu cgaaggcaug aguggaggca      660

ccugggucga cguaguacua gaacacggag gcugcgucac aguaauggca caagacaaac      720

cgacaguaga caucgagcua guuacaacaa ccguaucuaa uauggccgaa guccgcucau      780

acugcuacga agccuccauc uccgacaugg cauccgacuc cagaugcccu acccaaggcg      840

aagccuaccu cgacaaacaa uccgacaccc aauacgucug caaacgcacc cucguagacc      900

gagggugggg caacgguugc ggccuguucg gaaagggauc ccuagucaca ugcgcaaagu      960

uugccugcuc caagaaaaug acgggaaaau caauacaacc cgaaaaccua gaauaccgca     1020

uaaugcucag cguccacggg ucacaacaca gcgguaugau agugaacgac acuggacacg     1080

aaacagacga gaacagagcg aaagucgaaa ucacaccgaa cuccccucgc gcagaagcca     1140

cgcuaggagg guucggauca cuuggacucg acugcgaacc ccgcaccggc cucgacuuca     1200

gcgacuuaua cuaccuaaca augaacaaca aacauuggcu aguccacaaa gaaugguucc     1260

acgacauacc ccuacccugg cacgccggcg cagacacagg aacaccccac uggaacaaua     1320

aagaagcccu cgucgaguuc aaagacgccc acgcgaaaag acaaaccguc guuguacugg     1380

gcagccaaga aggcgccguc cacaccgcac uagcgggggc ucuagaagca gaaauggaug     1440

gcgccaaggg gcgacuauca ucaggccacc ucaaaugcag acuaaaaaug gacaaacucc     1500

gccucaaagg aguaucauac ucacuaugca ccgccgcguu cacauucacc aaaauacccg     1560

cugaaacucu ccacggaaca gucaccgucg aaguccaaua cgcaggaacc gacggccccu     1620

gcaagguacc agcccaaaug gccguagaca ugcaaacacu aacccccguc ggaagacuaa     1680

ucaccgcgaa ccccguaaua acagaaucca cagagaacuc aaaaaugaug cucgaacuag     1740

acccaccguu cggagacucu uacauaguca uaggagucgg agagaaaaag aucacacacc     1800

acuggcaccg cucaggcuca acaauuggca aagccuucga agccacggua cggggagcca     1860

aaagaauggc cguacuaggg gacacagccu gggacuucgg aucagucggc ggugcacuaa     1920

auucacuagg aaaaggcauc caccagaucu ucggcgcagc cuucaaaagc cuauuuggug     1980

gaaugucaug guucagccaa auccucauag ggacccuccu cauguggcuc ggccucaacg     2040

cuaaaaacgg aagcaucucg cuaaugugcc uagcccucgg gggcguccua auauuccuca     2100

gcacggcagu cagcgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10607
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10607
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau acuguucuua guagccgccg ccacgcgagu ccacagcaua acacgcaggg      120

gaucagcaua cuacauguac cucgaccgau ccgacgccgg aaaagcaaua ucauucgcaa      180

ccacgcuagg agucaacaaa ugccacgugc aaaucaugga ccuaggucac augugcgacg      240

ccaccauguc cuacgagugc ccuaugcucg acgaaggagu agaaccagau gacgucgacu      300

gcugguguaa cacaaccucc acaugggucg ucuacggcac augccaccac aagaaaggag      360

aagccagacg aagccgucgc gccgucacac uaccaagcca cagcacaaga aaacuccaaa      420

cccggucaca aacauggcuu gaaagccgag aauauacaaa acaccucaua aaaguagaaa      480

acuggaucuu ccggaaccca ggguucgcgc ucguagccgu ggcaauagcc uggcuccucg      540

gauccagcac aucccaaaaa gucaucuacc uaguaaugau ccucuugaua gcacccgccu      600

acucaauacg augcauagga gucagcaacc gcgauuucgu agaaggaaug uccggcggaa      660

caugggucga cgucguccuc gaacacggug gaugcgucac cgucauggca caagacaaac      720

ccacagucga cauagagcua gucaccacaa cuguaagcaa cauggccgaa guacgcuccu      780

acugcuauga agcauccaua uccgacaugg caucggacag ccgcugccca acgcaaggcg      840

aagccuaccu agacaaacaa ucagauaccc aguacgucug caaaaggacc cuaguagacc      900

gaggaugggg caacggaugc ggacucuucg gaaaaggcag ccuaguaacc ugcgcaaaau      960

ucacaugcuc caagaaaaug acaggaaaaa gcauacaacc ggaaaaccua gaguacagga     1020

ucaugcuaag cgugcacggc ucccaacacu caggaaugau aggcuacgaa accgacgaag     1080

accgagcaaa agucgaagua acacccaacu caccccgggc agaagcaacc cuagggggau     1140

ucggaucccu aggacucgac ugcgaacccc gcacaggacu ggacuucucc gaccuauacu     1200

accuaacgau gaacaacaaa cacugguuag uccacaaaga gugguuccac gauauccccc     1260

uaccauggca cgccggagcc gacacaggaa cgccacacug gaacaacaaa gaagcccuag     1320

ucgaguucaa agacgcacac gccaaaaggc agacagucgu agugcuaggc ucgcaagaag     1380

gggcagucca caccgcacuc gccggagcac uagaagccga aauggacgga gccaaggggc     1440

ggcuauucuc aggccaccug aaauguagac uuaagaugga uaaacugcga cuaaaaggag     1500

ugagcuacuc ccucugcaca gccgcauuca cguucacaaa aguaccggcc gaaacacugc     1560

acgggacagu caccguugaa guccaauacg cagggacuga cggccccugc aaaauccccg     1620

uccaaauggc cgucgacaug caaacccuaa cccccguagg acgacuaauc acggcaaacc     1680

cuguaauaac agaaucaaca gaaaacagca aaaugaugcu ugaacuagac ccaccauucg     1740

gagacuccua caucguaauc ggaguaggug acaagaagau aacacaccac uggcacagaa     1800

gcggcuccac aaucgggaaa gcguucgaag ccacaguccg cggcgccaaa agaauggcug     1860

uacuaggaga caccgcaugg gacuucggcu ccguaggcgg agucuucaac ucgcuaggga     1920

aaggaauaca ccaaauauuc ggagcagccu ucaaaucccu auucggagga auguccuggu     1980

ucucgcaaau ccuaaucggc acacuccugg ucuggcuagg acuaaacacg aaaaauggaa     2040

gcauuucccu aacaugccuc gcccucggag gaguaaugau auuccucagc accgccguaa     2100

gcgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10608
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10608
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau acuauuccuc guagccgccg caacacgagu ccacagcgua accaggcgcg      120

gaucagccua cuacauguac cuggaccgaa acgaugccgg cgaggcgaua ucauucccca      180

caacccucgg caugaacaaa ugcuacaucc agaucaugga ccucgggcau augugcgacg      240

ccaccauguc cuaugaaugc ccgaugcucg augaaggagu cgaaccagac gacgucgacu      300

gcuggugcaa caccaccagc accugggucg uauacggaac cugccaccac aagaaaggcg      360

aagcccgccg aagcagacgc gcagucacac uaccaagcca cucaacacga aaacuacaaa      420

cccgaucaca aaccuggcuc gaaucccgag aauacacaaa acaccuaauc cgagucgaaa      480

acuggauauu ccgaaaccca ggauucgccc ucgcggccgc cgccauagcc uggcuacugg      540

gauccagcac aagccaaaaa gucaucuacc uugucaugau ccuacuaauc gccccagccu      600

acuccauacg augcaucgga gucagcaaca gagacuucgu cgaaggcaug ucaggaggaa      660

cauggguaga cguaguacuc gaacacggcg gcugcguaac cguaauggcc caagacaaac      720

ccacaguaga cauagaacuu guaaccacca cagucuccaa cauggcagaa guccgcucau      780

acugcuacga agcauccauc agcgacaugg caagcgacuc acgcugccca acccaaggcg      840

aagcauaccu ggacaaacaa agcgacacgc aauacgucug caaaagaaca cucguggacc      900

gcggaugggg caauggaugc ggccucuucg gcaaggggag ccuagugacc ugcgcaaaau      960

ucgccuguuc aaagaaaaug accggcaaaa gcauacaacc ugagaaccuc gaauaccgca     1020

uuaugcugag cguccacggg agccaacaca gcggcaugau cguaaacgac accggucacg     1080

aaaccgacga aaaccgcgcc aaagucgaga ucaccccaaa cucaccccgc gccgaggcua     1140

cacugggagg auucggcuca cuagggcuug acugcgaacc gagaacaggc uuagacuuuu     1200

cagaccuuua cuacuuaacc augaacaaca aacacuggcu cguccacaaa gaaugguuuc     1260

acgacauccc ccugccaugg cacgccggcg cugacaccgg uacaccacac uggaacaaca     1320

aagaggcccu aguagaguuc aaagacgccc acgcaaaacg ccaaaccgua gucguacuag     1380

gcucccaaga aggagcagua cacaccgccc uggccggagc acuagaagca gagauggacg     1440

gagccaaggg gcgccucagc uccgggcacc ucaaaugucg ccugaaaaug gacaagcuaa     1500

ggcuaaaggg cguauccuac ucccuaugca cagccgccuu caccuucacc aaaaucccag     1560

cagaaacccu ccacggaacg gugacugugg aaguccaaua cgcaggaacc gauggacccu     1620

gcaaaguacc cgcacagaug gcgguggaca ugcaaacccu aacgcccguc ggacgccuca     1680

uaaccgccaa cccagucaua acagaaucaa cagaaaacag caaaaugaug cucgagcucg     1740

acccacccuu cggagacagc uacaucguca ucgggguagg cgaaaagaaa auaacccacc     1800

acuggcacag aucaggaucu acaaucggaa aagccuucga agccaccguc cgcggugcga     1860

aacgaauggc cguccuaggc gacaccgccu gggacuucgg cagcgucggg ggcgcacuca     1920

acucccuagg aaaaggcauc caccaaaucu ucggcgccgc cuucaaaagc cucuucggag     1980

gcaugucaug guucagccag auccucauag gcacacuccu aauguggcua ggccucaaca     2040

ccaaaaacgg gucaauuagc cuaaugugcc ucgcacuggg aggcgugcua aucuuccuaa     2100

gcaccgcagu cucggcauga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10609
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10609
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aaagaagcgc aggaucaaua auguggcuag cuucccuggc cguaguaauc gccugcgcag      120

gcgcaguaac acgucgaggg uccgcauacu acauguaccu cgauaggaac gacgcaggag      180

aggcaaucuc uuuccccacc acacuaggca ugaacaaaug cuacauccaa aucauggacc      240

uaggacacau gugcgacgcc acuauguccu acgaaugccc caugcuagac gaaggaguag      300

aaccggacga cguugacugc uggugcaaca ccacaucaac cugggucguc uacggaacau      360

gccaccacaa gaaaggagaa gccagacgaa gcagacgagc cguuacacua ccaucccaca      420

gcacaagaaa acuacaaacc agaucacaga ccuggcuaga aagcagagaa uacaccaaac      480

aucuaauccg agucgaaaac uggaucuuca gaaaccccgg cuucgcccug gcagcagccg      540

caauagccug gcuccucggc agcaguacau cccaaaaagu gauauaucuc gucaugauac      600

uauugaucgc acccgcauac agcaucagau guauaggcgu cagcaaccgu gacuucguag      660

agggaaugag cggcgggacc ugggucgaca ucguccugga acacggcgga ugcguaaccg      720

uuauggcaca ggacaaaccc accguagaca ucgagcuagu aacaaccaca gucuccaaca      780

uggccgaagu acgcuccuac ugcuacgaag cguccauaag cgacauggca agcgacagcc      840

gcugcccaac ccaaggggag gcauaccuag acaaacaaag cgacacucag uacgucugua      900

aaagaacacu cguagacaga ggauggggca auggaugcgg ccuauucgga aaaggcagcc      960

ucguaaccug cgccaaauuc gcaugcagua agaaaaugac cggaaaaagc auacaacccg     1020

agaaucuaga guaccggauc augcucagcg uacacggcuc ccaacacucc ggaaugauag     1080

uaaacgacac aggacacgaa acagacgaaa accgggccaa agucgagauc acaccaaacu     1140

ccccaagggc cgaagccacc cugggcgguu ucggcagccu aggacuagac ugcgaacccc     1200

gcaccggccu cgacuucagc gaccuauacu accuaaccau gaacaacaaa cacugguuag     1260

uccacaaaga augguuccac gacauacccc ucccguggca cgcaggcgca gacaccggca     1320

ccccucacug gaacaacaaa gaagcacucg ucgaguucaa agacgcccac gccaaaaggc     1380

aaaccgucgu cguacucggg ucacaagaag gagccguaca caccgcacua gccggagccc     1440

uagaagcaga aauggacggc gcaaaaggga gacuauccuc cggccaccuc aaaugccgac     1500

ugaaaaugga uaaacuacga cuaaaaggcg ucagcuacag ccugugcacc gcagcauuca     1560

ccuucaccaa gauacccgcc gaaacccucc acggaacagu caccgucgaa guccaauaug     1620

caggaacaga cggccccugc aaaguccccg cacaaauggc cguggacaug caaacccuua     1680

caccaguagg aagacucauc acagcaaacc cggucaucac cgaaagcacg gaaaacucga     1740

agaugaugcu cgagcuagac ccgcccuucg gagauagcua uauaguaaua ggaguuggag     1800

aaaagaaaau aacccaccac uggcacagaa gcggguccac aaucggaaaa gcauucgaag     1860

cgacaguacg aggggccaaa cgaauggcag uccucggaga cacagccugg gacuucggau     1920

ccguaggagg agcccuaaac ucgcucggca aaggcauaca ccaaaucuuc ggcgccgcgu     1980

ucaaaucccu auucggaggc augagcuggu ucagccaaau acuaauaggu acccuacuca     2040

uguggcucgg acugaacacc aaaaacggaa gcaucagucu uaugugccua gcacucggag     2100

gcguacuaau auuccuauca acagccguaa gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10610
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10610
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

aaagguccgc cggaagcaua auguggcugg ccagccuagc agucgugauc gcaugcgccg      120

gcgcgguaac acgccggggc ucggcauacu acauguaccu cgaccgaaac gacgccgggg      180

aagccaucag cuucccgacc acucucggga ugaacaagug cuacauacag auaauggacc      240

uuggacauac cugcgacgca acaaugagcu acgaaugccc aauguuagac gagggaguag      300

aaccagacga cguggacugc uggugcaaca caacguccac cuggguaguc uacggaaccu      360

gccaccacaa gaaaggcgag gcgcgaagaa gccgccgagc cgucacacua cccagucacu      420

caacacggaa acuacaaaca agaucacaaa cauggcuaga gagcagggaa uacaccaaac      480

accucauccg agucgagaac uggauauuca gaaacccagg cuucgcccuc gccgccgcgg      540

cgauagccug gcuacucggg agcuccacaa gccagaaagu aauauaccua gugaugauac      600

uccuaauagc gccugcauac ucgauccgau gcauuggagu aagcaaccgc gacuucgugg      660

aaggcauguc aggcggaacc uggguagacg uaguccuaga gcacggaggc ugcguaaccg      720

uaauggccca ggacaaaccc acagucgaca ucgagcuagu cacgacaacc gucagcaaca      780

uggcagaagu acguagcuac ugcuacgaag caucaaucuc agacauggca ucagacaguc      840

gaugccccac acaaggagaa gccuaccuag acaaacaguc agacacacaa uacgucugca      900

aaaggacccu cgucgaccga ggcuggggga acgggugcgg auuguucggc aaaggauccc      960

ucguuacaug cgcaaaauuu gcauguucga agaaaaugac ugggaaauca auacaaccgg     1020

aaaaucugga auacagaauc augcuauccg uacacggcag ccagcacagc gguaugaucg     1080

ucaaugacac cggccacgaa acugacgaaa acagagcaaa agucgaaauc acgcccaaca     1140

gcccacgggc ggaggccaca cucggaggcu ucggaucacu aggccuagac ugcgaaccac     1200

gcaccggccu agacuucagu gaccuauauu accugaccau gaacaacaaa cacuggcucg     1260

uacacaaaga augguuccac gacauacccc uaccguggca cgcgggggcc gacaccggga     1320

caccacacug gaacaauaaa gaagcccuag uagaguucaa agacgcgcac gccaaacgcc     1380

aaacagucgu aguacucggc ucacaagaag gagccgucca caccgcacuc gcaggagcuc     1440

ucgaagcaga aauggacgga gccaaaggac gacuuagcag cggacaccua aaaugcagac     1500

ucaaaaugga caaacucaga uuaaaaggag ucagcuacuc gcuaugcaca gcagcauuca     1560

ccuucacaaa gauccccgcg gaaacccuac acgggacggu cacaguagaa guccaauacg     1620

ccggcacgga cgggccaugc aagguucccg cacaaauggc ggucgacaug cagacacuga     1680

caccagucgg cagacuaaua accgcgaacc ccguaauaac agaaagcaca gagaacucga     1740

aaaugaugcu ggaacuagac ccaccguucg gagacucaua uauugugauc ggaguaggag     1800

aaaagaaaau aacacaccac uggcaucgca gcgggagcac aaucggaaaa gcauucgaag     1860

cuacaguccg cggcgccaaa agaauggcag ugcucggcga cacggccugg gacuucggau     1920

caguaggcgg agcccuaaac ucacuuggaa aaggaauaca ccaaauauuc ggcgcagcgu     1980

ucaaaagucu cuucggcggg augucauggu ucagccaaau ccucaucggc acccuacuaa     2040

uguggcucgg ccuaaaugca aagaacggca gcauaagccu caugugccua gcccuaggag     2100

gaguacuaau cuuccuaagu accgccguaa gcgcaugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10611
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10611
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

agcgaucagc cggauccauc auguggcucg ccagccuagc aguagucauc gcaugcgcag      120

gggccaucac aagacgaggc uccgcguauu acauguaccu agaccguagc gacgccggaa      180

aggcaaucag cuucgcaacc acccugggag ucaacaaaug ucacguacaa aucauggacc      240

uaggacauau gugcgacgca accaugagcu acgaaugccc caugcuagac gaaggcgucg      300

aacccgacga cguagacugc uggugcaaca caacuucaac augggucguc uacggaacau      360

gccaccacaa aaagggcgaa gcaagacgcu cccguagagc cgugacccug ccaagccacu      420

caaccagaaa acugcaaaca agaucacaaa cauggcuaga aagccgcgag uacacgaaac      480

accucauaaa aguagaaaac uggaucuucc gcaaccccgg guucgcacuc guagccgucg      540

caauagccug gcugcucggc uccagcacau cccagaaggu aauauaccua gucaugaucc      600

ugcucaucgc gccagcauac uccauacgau gcaucggcgu cagcaacaga gauuucguag      660

agggaaugag cggcggcacc uggguagaug ucguccuaga acacggaggc ugcgucaccg      720

uaauggcaca ggacaagccc acaguagaca uagagcuagu cacgacaaca gucagcaaca      780

uggcagaagu aagaagcuac ugcuaugaag caucuaucag cgacauggcg agcgacagcc      840

gaugccccac gcaaggcgag gccuaccucg acaagcaguc cgacacucaa uacguaugca      900

aacgaacccu cguagaccgg gguuggggaa acggaugcgg gcuauucgga aaaggcuccc      960

uaguaaccug cgccaaauuu accugcucca agaaaaugac cggaaaaucg auccaaccag     1020

aaaaccugga auauagaaua augcucagcg uccacggcag ccaacauucu ggcaugauag     1080

gcuacgaaac cgacgaggac agggcaaaag ucgagguaac gccaaacagc ccaagggcag     1140

aagcgacccu cggaggauuc ggcagccucg gacucgauug cgaaccccgc acaggccucg     1200

acuucucaga ccucuacuac cucacaauga acaacaaaca cuggcucgua cacaaggaau     1260

gguuccacga cauaccacug cccuggcaug ccggcgcuga uacaggaaca ccacacugga     1320

acaacaaaga agcccucgug gaguucaaag acgcccacgc aaaacgccaa accguaguag     1380

uccuaggaag ccaagaaggc gcgguacaca cagcccucgc cggagcccua gaagcagaaa     1440

uggacggagc caaagguaga cuauucucug gacaccucaa augccgauua aaaauggaca     1500

aacuccgacu aaagggugua ucauauagcc uaugcacagc agccuucaca uucacaaagg     1560

ugccagcaga aacgcuucac gggaccguca ccgucgaagu acaguacgcc ggcacagacg     1620

gaccgugcaa aauaccaguc cagauggcag uagacaugca aacacucaca ccggucggca     1680

ggcuaauaac agccaaccca guaauaacag aaagcacaga gaacagcaag augaugcuag     1740

aguuagaccc gcccuucggg gacagcuaca uaguaauagg cgucggagac aagaaaauaa     1800

cacaccacug gcaccgauca ggcagcacga ucggaaaagc cuucgaagca accguaaggg     1860

gcgccaaaag aauggcagua cuuggagaca cagcauggga cuucggaagc gucggcggag     1920

uauucaauag uuuaggcaaa ggcauacacc agauauucgg agccgccuuc aaaucacuau     1980

ucggagggau gucaugguuc agccaaaucc ucauaggcac ccuacucgua uggcuaggcc     2040

ucaacaccaa aaauggcucc auaagucuga caugccucgc cuuaggcggc guaaugauau     2100

uccuaucaac cgccguaagc gccugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10612
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10612
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aacgcucagc gggaucaaua auguggcucg ccucccuagc agucgucauc gccugcgccg      120

gagccgucac ucgccgcggc uccgcauacu acauguaccu cgaccgaaac gacgccggag      180

aagccauauc auuccccaca acccucggaa ugaacaaaug cuacauccaa aucauggacc      240

uaggccauau gugcgacgca acgaugucau acgaaugccc caugcucgac gaaggaguag      300

aacccgacga cguagacugc uggugcaaca caacgagcac cugggucguc uacgguaccu      360

gccaccacaa gaaaggcgaa gcccgcagau ccagacgagc aguaacucuc ccaucccaca      420

gcacccgcaa acuacagaca aggucgcaaa ccuggcucga aagcagagaa uacacaaaac      480

accucauccg cguagagaac uggaucuucc gaaaucccgg uuucgcccua gcagccgcag      540

caauagcaug gcuccucggc ucaagcacca gucaaaaagu aauauaccua gucaugauac      600

uccuaauagc acccgcauac agcauaagau gcaucggcgu aucgaaccgc gacuucgucg      660

agggaauguc cggaggaacg uggguugacg uaguacuaga gcacggcggg ugcguaacug      720

uaauggccca agacaaaccc acaguugaca uagagcucgu caccacaacg gucucaaaca      780

uggccgaagu ccggaguuac ugcuaugaag cgagcaucag cgacauggca uccgacuccc      840

gaugccccac acaaggcgaa gccuaccuag acaaacaguc agacacacaa uacgucugca      900

aacggacacu cgucgacaga ggauggggca acggcugcgg ccucuucggc aaaggcagcc      960

uugucaccug ugcgaaauuu gccugcagca agaaaaugac cggcaaaucc auacaacccg     1020

aaaaccugga auaccgaauc augcugucag uccacggauc ccaacacagc ggaaugaucg     1080

uuaacgacac aggucacgaa accgacgaaa auagagccaa aguagaaaua acaccgaaca     1140

gcccccgagc cgaagccaca cuaggagguu uuggcucccu cggccucgac ugcgagccac     1200

gcaccggacu cgauuucuca gaccucuacu acuuaacgau gaacaacaaa cacuggcucg     1260

uccacaaaga augguuccac gacauccccc ucccauggca cgcaggagca gacacaggca     1320

caccacacug gaacaacaag gaagcccuag ucgaguucaa agacgcccau gccaaacgcc     1380

aaaccguagu cgugcucggu ucacaagaag gcgccgucca cacggcacuc gccggagcac     1440

ucgaagccga aauggacggc gcaaagggac gacuauccuc aggccaccuc aaaugcagac     1500

ucaaaaugga caaacuaagg cuaaaaggcg uaagcuacuc ccuauguaca gcagcauuca     1560

cauucaccaa aaucccagca gaaacacuuc acggcacagu cacaguagaa guccaauacg     1620

ccgggacaga cgggccaugc aaagugccgg cacagauggc cguagacaug caaacccuaa     1680

cccccguagg ccgucucauc accgccaacc caguaaucac cgaaagcacc gaaaacucca     1740

aaaugaugcu agaacucgac ccacccuucg gggacaguua caucguaauc ggaguagggg     1800

aaaagaaaau cacccaccac uggcaccgau ccggcuccac caucgggaag gcuuucgaag     1860

caacgguacg cggcgcaaaa cggauggcgg uacucggaga cacagccugg gacuucggca     1920

gcgucggcgg agcccugaac ucacuaggga aaggaauaca ccaaaucuuc ggcgccgccu     1980

ucaaaucgcu auucgggggc augagcuggu ucucacaaau cuuaaucgga acccuacuca     2040

ugugguuggg ccugaacacc aagaacggau ccaucucacu gaugugucua gcucucggcg     2100

ggguacucau cuuccugagc accgcaguca gcgccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10613
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10613
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucucaggcgu accaguccua ggguucuuca ucaucgccgu ccucaugagc gcccaagaau      120

ccugggccgu aacccgccga ggauccgccu acuacaugua ccuagacaga aacgacgcag      180

gagaagccau cuccuucccg acaacacucg gaaugaacaa augcuacaua caaaucaugg      240

accucggcca caugugcgac gcaacaauga gcuacgaaug ucccaugcua gacgaaggag      300

ucgaacccga cgacgucgac ugcuggugca acacuaccag caccugggug gucuacggaa      360

ccugccacca caaaaagggc gaagcacgac gaaguagacg cgccgucacc cuccccagcc      420

acucaacacg uaaacuacaa acccgaagcc agaccuggcu cgaaagccga gaauacacca      480

aacaccucau cagaguagaa aauuggaucu ucagaaaccc cggguucgca cuggcggcag      540

ccgccaucgc cuggcuacuc ggaucuagca cgucccaaaa ggucauauac cuggucauga      600

uccuacuuau cgccccagca uacuccaucc gcuguauagg cguauccaac cgugacuuug      660

ucgaaggcau guccggcgga accugggucg acauaguccu cgaacacggc ggauguguca      720

ccguaauggc acaggacaaa ccaacagucg acaucgagcu aguaaccacc accgucucca      780

auauggcaga aguaagaucc uacugcuacg aagccucaau cagcgacaug gccucagaca      840

gccgaugccc cacacaaggc gaagcauacc uagauaagca gucagacacc caguacgucu      900

gcaagcgcac ccuagucgac cgcggauggg gaaacggcug cgggcuauuc ggaaaaggaa      960

gccuaguaac cugcgccaag uucgcuuguu caaagaaaau gaccggaaaa uccauacaac     1020

cagaaaaccu cgaauaccgc aucaugcuau ccguacacgg cucacaacac agcggcauga     1080

uaguaaacga caccggacac gaaaccgacg aaaaccgggc caaagucgaa auaacaccga     1140

auagcccccg cgccgaagca acccucggcg gauucggauc ccucggguua gacugcgagc     1200

cccgcacugg ccucgacuuc uccgaccuau acuaccucac caugaacaac aaacacuggc     1260

ugguccacaa agagugguuc cacgacaucc cccugccaug gcacgcagga gccgacaccg     1320

gcacacccca cuggaacaau aaagaagcuc uaguagaguu caaggacgca caugcaaaac     1380

gacaaaccgu cgucguccua ggcucccaag aaggagcagu acacaccgcc cucgcaggag     1440

cccucgaagc cgaaauggac ggagccaaag ggcgacucuc cucaggacac cuaaaauguc     1500

gccuaaaaau ggacaagcua aggcucaaag gaguaagcua cucccucugc accgcggcau     1560

ucaccuucac aaaaaucccc gcugaaacac uccacggcac cgucacagug gaaguacaau     1620

acgccggaac agacgggccg ugcaaaguac cagcccaaau ggcggucgac augcagaccc     1680

ucaccccagu cgggcgccuc aucacggcca acccaguaau uacggaauca accgagaacu     1740

ccaaaaugau gcucgaacug gaucccccau ucggcgacag cuacaucguc aucggcguug     1800

gcgaaaagaa aauaacacac cacuggcaca gauccggcag cacacucggc aaagcguucu     1860

cuacaacacu caaaggugca caacgacuag cugcacucgg cgacacagca ugggacuucg     1920

gauccaucgg cggagucuuc aacuccaucg ggagagcagu ccaccagguu uucgggggcg     1980

ccuuccgaac acuauucgga gggaugagcu ggaucacgca aggacuaaug ggagcccucc     2040

uacuauggau gggcguaaau gcaagagacc ggagcauagc acucgccuuc cuagcaaccg     2100

gaggcguccu cgucuuccua gccacaaacg uccacgcgug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10614
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10614
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu ccccguccua ggcuuuuuca ucauugccgu acucauguca gcccaagaaa      120

gcugggccgu aacccgacgc ggcagcgccu acuacaugua ccuagaccga aacgaugcag      180

gggaagcaau cuccuucccc accacgcuag gaaugaacaa augcuacauu caaauaaugg      240

accucggcca uaccugcgac gccaccaugu cauacgagug ccccauguua gacgaaggag      300

uagaacccga cgauguggau ugcuggugca acacaacaag caccuggguc guguacggga      360

ccugccacca caagaaagga gaagcgcgca gaucacgcag ggcagucacg cuacccagcc      420

acucaacacg gaaacugcaa acccgcuccc aaacauggcu agagucacgg gaauacacca      480

aacaccugau ccgagucgaa aacuggaucu uuagaaaccc aggauucgcc cucgccgcag      540

cugcuaucgc cuggcuccuc gggucaucaa caucccagaa aguuaucuau cucguaauga      600

uccuccuaau cgcccccgcc uacuccaucc gcugcauagg agugaguaac agagacuucg      660

uggaaggcau guccggcggg acauggguag acgucguacu cgaacacggg ggaugcguaa      720

cagugauggc ucaagauaaa ccaacaguag acaucgaacu cguuacgaca accgucagca      780

acauggcaga aguccgaagc uacugcuacg aggcaagcau aagcgacaug gccucagaca      840

gccgaugccc uacccaaggc gaagcauacc uagacaaaca aagcgacacc caauacgucu      900

gcaaaagaac gcucguggac cguggcuggg gaaacggcug cggccuauuc ggaaaaggaa      960

gucugguaac cugcgccaaa uucgcgugcu ccaagaaaau gaccggcaaa uccauccaac     1020

cugaaaaccu cgaauaccgg aucaugcuau ccguccacgg cagccaacac ucaggcauga     1080

ucgucaacga cacaggacac gaaaccgacg aaaaucgagc caaaguagaa auaacaccca     1140

acagcccaag agcagaagca acacuaggcg gauucgggag ccucggacuc gacugcgagc     1200

cacgcacagg gcuagacuuc ucagaccucu acuaccuaac caugaacaac aaacacuggc     1260

ucguccacaa agaaugguuc cacgacauac cacuccccug gcacgcaggg gccgacaccg     1320

gcacgccaca cuggaacaac aaagaggcac uuguagaguu caaagacgcc cacgcaaagc     1380

gacaaaccgu cguaguucuc ggaucccaag aaggcgccgu ccacacggcc cucgccggag     1440

cacuagaagc agaaauggac ggcgccaaag gaaggcuaag uuccggccac cucaaaugua     1500

gacucaaaau ggacaaacuu cgacuaaaag gagucagcua cagcuuaugc accgccgcau     1560

ucacauucac aaaaauccca gcagaaacac uacacggaac cgucacaguc gaaguacaau     1620

acgcgggcac agacggccca uguaaggugc ccgcccaaau ggcaguggac augcaaaccc     1680

uaacccccgu agggcgacuc aucacggcaa acccagucau caccgaaagc acagaaaaca     1740

gcaaaaugau gcucgaacuc gacccaccuu ucggggacuc cuacauaguc aucggcgucg     1800

gcgaaaagaa aauaacccac cacuggcaca gaucaggcag uacccuugga aaagccuucu     1860

caaccacuuu aaaaggugca caaagacuag cugcacucgg cgacaccgca ugggacuucg     1920

gcucgauagg cgggguauuc aacagcauag gacgagcggu ccaccaaguc uucggcggcg     1980

cauuccggac acuguucgga ggaauguccu ggauaaccca gggacucaug ggggcccuac     2040

uccucuggau gggaguaaac gcgagagacc gcuccaucgc ccuagcauuc cuagccacag     2100

gaggcguccu aguuuuccuc gcaacaaacg uacaugccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10615
<211>  2458
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10615
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucucaggggu accaguacua ggcuucuuca ucauagccgu ccucauguca gcccaagaau      120

caugggcgau aacacgccga ggcagcgcuu acuauaugua ucucgaucga agcgacgccg      180

gaaaagccau caguuucgcu accacucuag gaguuaacaa augccacgua caaaucaugg      240

aucugggcca caugugcgac gccaccauga gcuacgagug cccaaugcuc gacgaaggag      300

uagaaccgga cgacgucgac ugcuggugca acaccaccag uacauggguu gucuacggca      360

cuugccacca caagaaagga gaagcccgac gaucccgccg cgcggucacu cuccccuccc      420

acuccacacg aaaacuccaa acccgcucac aaacguggcu agagucgcga gaauacacca      480

aacaccucau aaaaguagaa aacuggaucu uccgcaaccc aggcuucgca cucguagcgg      540

uugcgaucgc guggcuccua ggcuccucca cuucccaaaa agucaucuac cuaguuauga      600

uccuccuaau cgccccagcc uacagcaucc ggugcaucgg agucucaaac agagacuucg      660

ucgaagguau gagcggcggc acauggguag acgugguccu cgaacacggc ggaugcguca      720

caguuauggc gcaagacaaa cccaccguag acaucgaacu cgucacgacc accgugucaa      780

acauggccga aguccgaagc uacugcuacg aagcaucaau aucagacaug gccuccgacu      840

cccguugccc aacgcaagga gaagccuacc ucgacaaaca aagcgacacc caauacguuu      900

gcaaacgcac ccuagucgac cgcggcuggg gcaacggaug cggccucuuc ggaaaaggcu      960

cccucgugac augcgcaaaa uucacaugca gcaagaaaau gacaggaaaa ucaauccaac     1020

ccgaaaaccu cgaauacaga aucauguugu caguacacgg cagccaacac ucaggaauga     1080

uaggauacga aacggacgaa gaccgcgcaa aagucgaagu caccccaaac agccccaggg     1140

ccgaggcgac ccuuggagga uucggcagcc ucggacucga cugcgagccc cgcacaggcc     1200

ucgauuucag cgaucuauac uaccuuacca ugaauaacaa acacugguua guccacaaag     1260

aaugguucca cgacauaccc cuccccuggc acgcgggagc ggacaccggu accccacacu     1320

ggaacaacaa agaagcacuc gucgaguuca aagacgccca cgccaaaagg caaacaguag     1380

uaguccucgg aagucaagaa ggcgccgucc acaccgcacu ggccggcgcc cucgaagccg     1440

aaauggaugg agccaaaggc cgacucuucu caggacaccu caaaugccga cucaaaaugg     1500

acaaacucag acuaaaagga guauccuaca gccuuugcac agccgccuuc acguucacaa     1560

aaguccccgc cgaaacacuu cacggaacgg ucacagucga aguacaauac gccggcaccg     1620

acggccccug caaaaucccc guucaaaugg cgguggacau gcaaacucuc acaccuguag     1680

gacgacuaau uaccgccaac cccgucauca cugaauccac agaaaacagc aaaaugaugc     1740

uagaacucga ucccccauuc ggcgacagcu acauaguaau cggugucggc gacaagaaaa     1800

ucacacacca cuggcaccgc agcggcucca cacuuggaaa agcauuuuca accacccuca     1860

aaggcgcaca aagacuagcc gcgcucggag acacagcaug ggacuucggc ucgaucggcg     1920

gagucuucaa cuccauagga cgagccgucc accagguauu cggcggagcc uuccgcacac     1980

uguucggagg caugagcugg aucacucaag gacucauggg agcgcuccua cucuggaugg     2040

gcgucaacgc ccgcgaccga agcauagcuu uagccuuccu cgccacagga ggcguccucg     2100

ucuuccucgc gaccaacgua cacgccugag gacuagugca ucacauuuaa aagcaucuca     2160

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     2220

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     2280

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2400

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2458


<210>  10616
<211>  2470
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10616
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcga       60

ucuccggcgu accaguccua ggauucuuca ucaucgcagu ccucaugucu gcacaagaau      120

caugggcagu aacaagacgc ggguccgcau acuacaugua ccucgaucga aacgaugccg      180

gcgaagccau auccuucccc acaacacuag gcaugaacaa augcuacauc caaaucaugg      240

accuaggaca caugugcgac gcaacaaugu ccuacgaaug cccaaugcug gacgaggggg      300

uggaaccaga cgacguagau uguuggugca acacaaccuc caccugggug guauacggaa      360

cgugccacca caaaaaggga gaagcccgac gcagccgacg agccgugacc cuacccuccc      420

acagcacacg aaaacuacaa acacgcagcc aaacauggcu agaaagcaga gaauacacca      480

aacaccuaau acgcguagaa aacuggaucu uccgcaaccc aggcuucgca cuggccgcag      540

ccgccaucgc auggcuccuc ggcagcucaa ccucccaaaa gguaaucuac cuuguuauga      600

uccuccuaau cgcaccggcg uacuccauaa gaugcaucgg cgucucaaau agggacuucg      660

uagaaggcau gagcggggga acguggguag acguaguccu cgaacacgga ggcugcguaa      720

ccgugauggc acaagacaaa ccaacgguag acaucgagcu aguaacaaca accgucucca      780

acauggcuga agucagaucc uacuguuacg aagccagcau aagugacaug gccucagacu      840

cacgcugccc cacacaaggc gaagcauacc uagacaaaca auccgacacc caguacgucu      900

gcaagcgaac ccucgucgac agaggcuggg gcaacggaug uggccuauuu ggaaagggaa      960

gccucguaac cugcgcuaaa uucgccugcu caaagaaaau gacaggcaaa agcauccaac     1020

ccgaaaaccu ggaauaccgc aucaugcuaa gcguccacgg cagccaacac ucagggauga     1080

uaguaaauga uaccggacac gaaacagacg aaaaccgagc aaaaguggaa auaacaccaa     1140

acucgccacg ggccgaagcc acgcuagggg gcuucggcuc acuagggcuc gauugcgagc     1200

caagaaccgg acuagauuuc agcgaccuau acuaucuaac gaugaacaac aaacauuggc     1260

uaguacacaa agaaugguuc cacgacaucc cccuaccaug gcacgccggc gccgacaccg     1320

gcacaccaca cuggaacaac aaagaagcac ucguggaguu caaagaugcc cacgccaaaa     1380

gacaaacagu cgugguacua ggcagucaag aaggcgccgu gcacacagcu cuugcgggag     1440

cccuagaagc cgaaauggac ggcgccaaag gaagacuaag cuccgggcac cucaaaugcc     1500

gacuaaaaau ggacaaacuc agacugaaag gcgucagcua cucacucugc acagcagccu     1560

ucacauucac caaaauuccc gcagaaacac uacacggcac aguaaccguc gaaguccaau     1620

acgcaggcac agacggcccc ugcaaagucc ccgcacaaau ggccguagac augcaaacac     1680

uaacacccgu aggucgacua auaaccgcga acccagucau cacagaaucc acugaaaaca     1740

gcaaaaugau gcuagaacuc gacccgccau ucggagacuc auacaucgua aucggagucg     1800

gagagaagaa aauaacacac cauuggcacc gcucgggcuc cacacuaggc aaagccuuca     1860

gcacaacccu caaaggagca caaagacugg ccgcacucgg cgacaccgcg ugggacuucg     1920

gcagcaucgg aggcguguuc aacucaauag gacgugccgu acaccaaguc uucggcggag     1980

ccuuccgaac acuauucggc gggaugucau ggauaaccca aggccuaaug ggcgcccuac     2040

uccuauggau gggaguaaac gcacgggacc gcucgauagc acuagcuuuc cucgcaacgg     2100

gcggcguccu aguguuucua gcaacaaacg uucacgccug aggacuagug caucacauuu     2160

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     2220

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     2280

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2340

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2460

caccagaauu                                                            2470


<210>  10617
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10617
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau acuauuccuc guagccgccg ccacccgggu ccauucaguc acccgccgag      120

gcucugccua cuacauguau cuggacagaa acgacgccgg cgaagcaauu agcuucccaa      180

caacccucgg aaugaacaaa ugcuacauac aaauaaugga ccucggccac augugcgacg      240

caaccauguc auacgagugc ccaaugcugg acgagggagu cgaacccgac gacguagacu      300

gcuggugcaa caccaccagc accugggucg uauacggcac augucaccac aagaaagggg      360

aagcccgacg uagccgcaga gcaguaaccc uaccaaguca uucaacacgc aaacuccaaa      420

caaggagcca aaccuggcua gaaucacggg aguacacaaa acaccucaua cgaguagaaa      480

acuggaucuu cagaaacccg ggguucgccc ucgcagccgc cgccauagca uggcuucucg      540

ggucaagcac cucccaaaaa guaaucuacc ucgucaugau acuacuaauc gcaccagcau      600

acagcauacg augcaucggc guaucaaaca gagacuucgu agaaggcaug aguggaggga      660

ccuggguuga cauaguacug gaacacggcg gaugcgucac agucauggca caggacaaac      720

caaccgucga cauagagcuu guuacaacca cggucagcaa cauggccgaa guccgaagcu      780

acugcuacga ggcaagcaua ucagacaugg ccucagacag ccgaugccca acgcagggag      840

aagcauaccu cgacaaacaa agcgacacgc aauacgucug caaaaggacg cuuguagacc      900

gcggcugggg aaacggaugc ggccucuuug gaaaaggauc ucuaguaacc ugcgcaaagu      960

ucgcgugcuc aaagaaaaug acuggaaaau caauccaacc cgagaaccuc gaauaccgca     1020

ucaugcuauc cguccacggg ucacaacaca gcggaaugau cguaaacgac acaggacacg     1080

aaacagacga aaacagggcc aaaguagaga ucaccccgaa cagcccaaga gccgaagcaa     1140

cacuaggcgg cuucggcagu cuuggacucg acugcgaacc gcggacugga cuggacuuua     1200

gcgaccugua cuaucucacc augaacaaca aacacuggcu aguacacaaa gaaugguucc     1260

acgacauccc gcucccaugg cacgcaggcg ccgauaccgg aaccccucac uggaacaaca     1320

aagaagcccu cguagaguuc aaagacgccc acgccaaacg acaaacagua gucgugcucg     1380

gaagccaaga aggagcggua cacacggcac ucgcgggagc ccuggaagcg gaaauggacg     1440

gcgcaaaagg acgacuauca agcggucacc ucaaaugccg acuaaaaaug gacaagcuac     1500

gccucaaagg cgucagcuau agccuaugca cagccgcauu cacauucaca aaaauaccgg     1560

ccgaaacacu acacggcacc gucaccgucg agguacaaua cgcagguacc gacggacccu     1620

gcaaaguccc agcccagaug gcagucgaca ugcaaacccu caccccuguc gggaggcuaa     1680

ucaccgcaaa ccccguaauu acagaaucaa cagagaacag caaaaugaug cuggaacugg     1740

acccacccuu cggcgacucc uacaucguca ucggaguagg ugaaaagaaa aucacccacc     1800

acuggcaccg aagcggcuca acccuaggca aagccuucag cacaacccua aaaggagccc     1860

aacgccucgc cgcccucggc gauacggcgu gggacuucgg cuccauagga ggcguauuca     1920

auucaaucgg ccgcgccgua caccaagucu ucggcggagc auuccggaca cucuucggag     1980

gaaugagcug gauaacgcaa ggacucaugg gcgcauuacu cuuauggaug gggguaaacg     2040

cccgagaccg uagcaucgcc uuagccuucc ucgccacagg cggagugcua guauuccucg     2100

caacaaacgu ccacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10618
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10618
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau acucuuccug guagccgcag cuacccgcgu acacucaguc acacgccgag      120

gaucggccua cuacauguac uuagaccgca acgacgcugg cgaagcaaua ucauucccca      180

caaccuuagg caugaacaaa ugcuacauac aaaucaugga ccugggccac acaugugacg      240

cgaccauguc cuacgagugc ccaaugcugg acgaaggagu agagccggac gacguagacu      300

gcuggugcaa caccaccagc acauggguag ucuacggaac cugccaccac aagaaaggcg      360

aggcccgccg aagccgaagg gcagucaccc ucccaagcca cuccacccga aaacuacaga      420

cccgcuccca aaccuggcua gaaucucgug aauacacaaa acaccucauc aggguagaaa      480

acuggaucuu ccgcaacccc ggcuucgcac ucgcagcagc cgcaaucgcu uggcuacugg      540

gcuccuccac cucacaaaaa guaauauacc uggucaugau ccuacugauc gccccagccu      600

acagcauccg cugcaucggc guaagcaacc gggacuucgu agaaggcaug ucuggcggga      660

ccuggguaga cgucgugcua gagcacggcg gcugcgucac aguaauggca caagacaagc      720

ccacagucga caucgaacuc gucaccacca ccguaagcaa cauggcagag guccgcuccu      780

auugcuacga ggccagcaua ucagacaugg caagcgacag ccggugccca acccaaggcg      840

aagcauaccu cgauaaacaa uccgacacgc aauacguaug caaacgaaca cucgucgaca      900

gaggcugggg aaacggaugc ggccuauucg gaaaaggauc ccuagucacc ugugccaaau      960

ucgccugcag uaagaaaaug acaggcaaaa gcauacaacc agaaaaccua gaauaccgaa     1020

ucaugcucuc cguacacgga agccaacacu caggaaugau cgucaacgac acugggcacg     1080

aaaccgauga aaaccgugca aaaguagaaa uaacgcccaa cucaccacgc gcagaagcca     1140

cccugggagg cuucggcagc cuaggacucg acugcgaacc aagaacuggc cucgacuucu     1200

cagaccucua cuaccucacc augaacaaca aacacuggcu cguacacaaa gagugguucc     1260

acgacauccc ccuaccaugg cacgcugggg cggacacagg cacaccccac uggaacaaua     1320

aagaagcccu aguagaguuc aaagacgcac acgcaaaacg acaaaccgua gucguacuug     1380

gcagccagga aggugcgguc cauaccgcac uagccggcgc ccucgaagcc gaaauggacg     1440

gcgcuaaagg ucgccucuca agcggccauc uaaagugccg acuaaaaaug gacaaacucc     1500

gucucaaagg agucuccuac agccugugca ccgcagcauu caccuucaca aaaaucccag     1560

cggaaacccu ccauggaacc gugaccgucg aaguccaaua cgcaggaaca gauggacccu     1620

gcaagguccc cgcccaaaug gcaguagaca ugcaaacacu gacccccguc ggccgacuca     1680

uaacggccaa cccagucaua acugaaucca cagaaaacuc aaaaaugaug cuggagcucg     1740

acccgcccuu cggcgauagc uacauaguca ucggcguggg cgaaaagaaa auaacucacc     1800

acuggcaccg cagcggcagu acacuaggca aagccuucag uaccacccug aaaggagccc     1860

aacgacucgc agcccucgga gacacagcau gggacuucgg uagcaucggc ggcgucuuca     1920

acuccaucgg gagagccgug caccaagugu ucggaggcgc cuuccguacc cucuucggag     1980

gaaugucaug gauaacucaa gggcucaugg gcgcccuccu acucuggaug ggaguaaacg     2040

ccagagacag aagcaucgcc cuagccuucc uagccaccgg cggaguacuc guauuccugg     2100

caaccaacgu ccacgcuuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10619
<211>  2437
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10619
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggaccuggau ccuguuccuc guagccgccg ccacacgcgu acauucaaua acacgccgag      120

gaucggcaua cuacauguac uuggaccgua gcgacgcagg caaagcgauc uccuucgcca      180

caacccuggg cgucaauaaa ugccacguac aaaucaugga ccuuggacac augugcgacg      240

ccacaauguc auacgaaugc cccaugcugg acgagggcgu agaaccagac gacgucgacu      300

gcuggugcaa uaccacaagc acaugggucg uuuacggcac augccaccac aagaaagggg      360

aagcgcgacg gucccgacga gcggugacac uaccauccca cagcacacgc aaacuacaaa      420

caaggagcca aaccuggcuc gaaagccgag aguacacaaa acaccuaauu aaaguagaaa      480

acuggaucuu ccgcaaucca ggauucgcgc uaguagcagu cgccauagcc uggcuacuag      540

gaagcagcac cucccagaaa gucaucuauc uagucaugau ccucuuaaua gcacccgcgu      600

acaguauacg augcauaggc guaagcaaca gggauuucgu agaaggaaug ucgggaggga      660

ccuggguaga cgucgugcuc gagcacggcg ggugcguaac cguaauggcc caagacaaac      720

ccacagucga caucgaacuc guuacaacca caguuagcaa uauggccgaa guaaggucau      780

acugcuacga agcaucgauc agugacaugg caucagacuc acggugcccc acgcaaggcg      840

aagcauaccu agacaaacaa agcgacaccc aauacgucug caaacgaaca cucgucgacc      900

gaggcugggg aaacgggugc ggacucuucg gcaaaggcuc gcugguaacc ugcgcaaaau      960

uuacauguuc aaagaaaaug acaggcaaaa gcauucaacc cgaaaaccuc gaguaccgaa     1020

ucaugcuauc aguccacgga agccaacaca gcggaaugau cggguaugaa accgacgagg     1080

acagagccaa aguagaagua acgccaaaca gcccccgggc cgaagccaca cugggcggau     1140

ucggaagccu cggccuggac ugcgaaccca gaacaggccu ggacuucagc gaccuauacu     1200

accuaaccau gaacaacaaa cacuggcucg uacacaaaga gugguuccac gacauccccc     1260

ucccauggca cgcaggagcc gacaccggca caccccacug gaacaacaaa gaagcccugg     1320

ucgaguucaa agacgcccac gcgaaacgac agaccguagu cgugcuagga agucaagagg     1380

gggcaguaca cacagcccuc gccggagcac ucgaggccga gauggacggc gcaaaaggac     1440

gccucuucuc gggacaccua aaaugccgcc ucaaaaugga uaaauuaaga cuaaaaggcg     1500

ucucauacuc guuaugcacc gccgcauuca ccuucaccaa aguccccgcc gaaacccuac     1560

auggaacagu aaccguggaa guacaguacg cggggaccga cggacccugc aaaaucccag     1620

uccagauggc ggucgacaug caaacccuga cgcccgucgg acgccuaaua acggcuaacc     1680

cggugauaac cgaauccaca gaaaacucaa aaaugaugcu cgaacuagac ccaccauucg     1740

gagacagcua caucguaaua ggagucggcg acaagaaaau cacccaccac uggcaccgaa     1800

guggauccac acuaggcaaa gcauucucaa ccacacucaa aggggcccag cggcucgccg     1860

cccuaggaga cacagcaugg gacuucggau caaucggagg uguauucaac ucgauaggca     1920

gagccgucca ccaaguauuc ggaggggcau uccgaacacu auucggggga augagcugga     1980

ucacacaagg acucauggga gcccugcugc uuuggauggg cguaaacgcc agagaccgau     2040

caauagcccu agcauuccuu gcaacaggag gcguccucgu cuuccuagca acaaacguac     2100

acgcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     2160

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     2220

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2280

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2400

ccccccccca aaggcucuuu ucagagccac cagaauu                              2437


<210>  10620
<211>  2449
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10620
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggacauggau ccucuuucuc guagcggccg caacccgagu ccacagcgug acccgccgcg      120

gcuccgccua cuacauguac cucgacagaa acgacgccgg cgaggcaauc uccuucccga      180

ccacccucgg aaugaauaaa uguuacauac aaaucaugga ccucgggcac augugcgacg      240

caaccaugag cuacgaaugc cccaugcugg acgaaggagu agaaccagac gacgucgauu      300

gcuggugcaa caccacaagc accuggguag uauauggcac augucaccac aaaaaggggg      360

aagcccgccg cucacgccgc gccguaacac ucccaucaca cagcacccgc aaacuccaaa      420

ccagauccca aaccuggcuc gaaucaagag aguacacaaa gcaccuaaua cgcguagaaa      480

acuggauuuu cagaaaccca ggcuucgcuc uugcagccgc cgcgaucgcc uggcuccucg      540

gcuccaguac aagccagaag gucaucuacc ucguaaugau ccuccuaauc gcaccagccu      600

acuccauacg cugcauaggu guaucaaacc gagacuucgu agaaggaaug agcggcggaa      660

caugggucga cguaguacua gaacacggcg gcugcgucac agucauggca caggauaaac      720

caacggucga caucgaacuc guaaccacaa caguaagcaa cauggcagag guccgaagcu      780

acugcuacga ggccuccaua uccgacaugg cauccgacuc caggugcccc acccaaggag      840

aagccuauuu agacaaacaa uccgacaccc aauacgucug caaaagaaca cugguggauc      900

gaggaugggg gaacggcugc ggauuauucg gaaaaggauc acucguaacc ugcgcaaaau      960

ucgcaugcag caagaaaaug acaggcaaaa gcauccagcc ggaaaaccuc gaauaccgca     1020

ucaugcuauc aguacacgga ucccaacacu ccggcaugau cgugaacgac acaggacacg     1080

aaacggacga aaaccgggcc aaggucgaaa uaacaccuaa cucaccccga gccgaagcga     1140

cucucggagg guucgggagc cucggccucg acugcgagcc acgcaccggc cuagacuuuu     1200

cagaccucua uuaccucacc augaacaaca aacacuggcu aguccacaaa gaaugguucc     1260

acgacauccc acuaccuugg cacgccggcg ccgacacagg cacaccccac uggaauaaca     1320

aagaagcacu ggucgaauuu aaagacgcac acgccaaacg acaaacaguc gucguacuag     1380

gaucacaaga aggagcaguu cacaccgccc uagccggagc acuugaagca gaaauggacg     1440

gagcaaaagg acgccuuagc ucaggacacc uaaagugcag gcugaagaug gacaaacuga     1500

gacucaaagg agucuccuac ucacugugca ccgcagccuu cacauucaca aagauccccg     1560

cagagacucu ccacggaacc gucaccgucg aaguacaaua cgcaggaaca gacggcccuu     1620

gcaagguccc cgcccaaaug gcagucgaca ugcagacacu caccccaguc ggaagacuca     1680

ucacagccaa ccccgucaua accgaaucca ccgaaaacuc aaaaaugaug cucgaacugg     1740

acccacccuu cggugacagc uacaucguca uagggguagg agaaaagaaa aucacacacc     1800

acuggcaccg cucaggaucc acccucggaa aagccuucuc aaccacacuc aaaggcgcac     1860

aaagacucgc ggcacucgga gacaccgcgu gggacuucgg auccaucggc ggcguauuca     1920

acagcauagg acgcgcaguc caccaaguau ucggaggagc cuuucgaaca cucuucggcg     1980

gaaugucaug gaucacccaa ggccuaaugg gcgcccuacu ccucuggaug ggagugaacg     2040

cccgcgaccg gagcaucgca cuagcuuucu uggcaacagg cggcguccua guauuccugg     2100

ccaccaacgu acacgccuga ggacuagugc aucacauuua aaagcaucuc agccuaccau     2160

gagaauaaga gaaagaaaau gaagaucaau agcuuauuca ucucuuuuuc uuuuucguug     2220

guguaaagcc aacacccugu cuaaaaaaca uaaauuucuu uaaucauuuu gccucuuuuc     2280

ucugugcuuc aauuaauaaa aaauggaaag aaccuagauc uaaaaaaaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaugcau cccccccccc     2400

cccccccccc cccccccccc caaaggcucu uuucagagcc accagaauu                 2449


<210>  10621
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 2 opt

<400>  10621
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

aaaggucagc gggcagcauc auguggcucg ccucccucgc uguagugauc gccugcgcag      120

gagcgguaac gcgccgaggc ucagcauacu acauguaucu ggaccgaaac gaugccggag      180

aagcaauauc cuucccaaca acacuaggca ugaacaaaug cuacauacaa auaauggacc      240

uaggccacau gugcgacgca acgaugucau acgaaugccc aaugcucgac gaaggcguug      300

aaccagacga cgucgacugc uggugcaaca caacgagcac gugggucgua uacggcacau      360

gccaccacaa gaaaggagaa gcacgccgga gccgucgggc agucacccug ccaucccacu      420

ccacaagaaa acuccaaaca cgaagccaga cauggcucga gagcagagaa uacaccaaac      480

accuaaucag agucgaaaac uggauauucc gaaaccccgg guucgcgcuc gcagccgccg      540

caaucgccug gcuacuagga ucaucaacca gccaaaaagu aaucuaucua guaaugauac      600

uacuaauagc cccggcguac uccauaagau gcauaggcgu caguaaccga gauuucguag      660

aaggaauguc aggagggaca ugggucgaca ucguacuaga gcacggcggc ugcgugaccg      720

uaauggcaca ggacaaacca acgguagaca ucgaacucgu caccacaacc guaaguaaca      780

uggcagaagu gagaucauau ugcuacgaag ccuccauaag cgacauggcc agcgacucua      840

gauguccaac gcaaggagaa gcguaccucg acaaacaguc cgacacgcaa uacgucugca      900

aacgcacacu cguggaccgc ggcuggggaa acggaugcgg acuauucgga aaaggguccc      960

uagucacaug cgcaaaauuu gcgugcucaa agaaaaugac aggcaaaucc auccagccag     1020

aaaaccuaga auacagaauc augcucuccg uacacggcuc ccaacacagc ggaaugaucg     1080

uaaacgacac aggacaugaa acggacgaga acagagcaaa agucgaaauc acgcccaaua     1140

gcccacgcgc cgaagcaacc cuuggaggcu ucggcagcuu gggccuugac ugcgaaccac     1200

gcacggggcu agacuucuca gaccuauacu accuaacaau gaacaacaaa cacuggcucg     1260

uccacaaaga gugguuccac gacauccccc ucccguggca cgccggcgca gauaccggca     1320

caccccacug gaacaacaaa gaagcccuag ucgaguucaa agacgcacac gccaaaagac     1380

aaacagucgu gguacuagga ucccaagaag gagcagucca cacagcgcua gcaggcgccc     1440

uagaggccga gauggaugga gcgaaaggac gucuauccuc aggacaccua aaaugcaggc     1500

uaaaaaugga caaacuacga cuaaaaggcg ucucuuacuc acuaugcaca gccgcauuca     1560

cauuuaccaa aaucccagcg gaaacacugc acggcacagu aaccguagaa guacaauacg     1620

caggcaccga cggacccugu aaggucccag cccaaauggc agucgacaug caaacacuga     1680

ccccggucgg acgccugaua acagccaacc caguaaucac agaaucgaca gaaaauucca     1740

aaaugaugcu agaacuagau cccccauucg gcgacagcua uaucgugauc ggaguagggg     1800

aaaagaaaau cacccaccac uggcaccgau ccgggagcac gcucggaaaa gcguucucaa     1860

ccacacugaa aggggcacag cggcucgcag cacucggcga caccgcaugg gacuucgggu     1920

caaucggagg cgucuucaac uccaucggac gagcaguaca ccaagucuuc ggaggcgcgu     1980

ucagaacauu auuugggggc augagcugga ucacacaggg ccucaugggc gcccuacucc     2040

ucuggauggg ggugaacgcc agagacagau ccauugcacu agcguuccua gcaacgggcg     2100

gcguacucgu auuccuagcc acaaacgucc augccugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10622
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 2 opt

<400>  10622
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggga       60

aaagaucagc aggcuccauc auguggcucg ccucacucgc aguagucaua gcaugcgcag      120

gcgcggucac cagacgaggc ucagcguacu acauguaccu ugacaggaac gacgcaggag      180

aagccaucag cuucccgacc acacucggca ugaacaaaug uuacauccaa aucauggacc      240

uaggacauac cugcgacgcc acaaugucau acgaaugccc aaugcuagac gaaggcguag      300

aacccgacga cgucgacugc uggugcaaca ccacgagcac cugggucguc uacggcacau      360

gccaccacaa gaaaggagaa gcccgccgau cccgccgagc agucacccuc cccagccacu      420

cuaccagaaa acuacaaacc agaagccaga ccuggcucga aucaagggaa uacacaaaac      480

acuuaaucag ggucgaaaac uggauauucc guaacccagg guucgcccuc gcggcagccg      540

ccauagcaug gcuacugggc ucgagcacua gccagaaagu aaucuaccuc gucaugauau      600

uacugaucgc accagccuac ucaaucagau gcauaggcgu aagcaaccgc gacuucguag      660

aagggauguc agguggaaca ugggucgacg ucguacucga acacggcggu ugcgucacag      720

uaauggcuca ggacaagccc acgguagaca uagaacuugu aacaacaacc guaagcaaca      780

uggccgaagu acgcuccuac ugcuacgaag ccucaaucuc agacauggca uccgacucca      840

gguguccaac gcaaggggaa gcguaccuag acaaacaauc cgacacacaa uacgucugca      900

aaagaacccu ggucgaccga ggcuggggca acggaugcgg gcuauuuggc aaaggcuccc      960

uaguaaccug ugccaaguuc gcaugcucca agaaaaugac cggcaaaucc auacaacccg     1020

aaaacuuaga auacagaaua augcuaucug uucacggauc ccaacacagc gggaugauag     1080

ucaaugacac aggacacgaa acagacgaga acagggccaa aguagaaaua acccccaaca     1140

gcccacgcgc agaagcuaca cugggcggau ucggaucucu aggccuagac ugcgaaccac     1200

gcaccggccu agacuucuca gaccucuacu acuugaccau gaauaacaaa cacugguuag     1260

uccacaaaga augguuccac gacauaccac uccccuggca cgccggcgca gacacaggca     1320

ccccacacug gaacaacaaa gaagcccucg ucgaguucaa agacgcacac gcgaaacggc     1380

agaccgucgu aguccucgga agccaagaag gagcggucca cacagcccua gcaggcgcac     1440

uagaagcaga aauggacggc gcgaaagggc gacuguccuc agggcaccuc aaaugucggc     1500

ucaaaaugga caaacuacgc cuuaaaggcg uaucguacuc acuaugcacc gcggccuuca     1560

cauucaccaa aauccccgcc gaaacacuac auggaaccgu aaccgucgaa guacaauacg     1620

caggcaccga ugggcccugc aaagucccug cacaaauggc agucgacaug caaacacuca     1680

ccccagucgg ccgacuaauc acagcuaacc ccgugauaac ggaguccaca gaaaacucga     1740

aaaugaugcu cgaacuagac ccgcccuucg gcgacagcua cauaguuauc ggagucggug     1800

aaaagaaaau aacccaccac uggcaccgaa gcggaagcac ccucggaaaa gccuucucca     1860

ccacccucaa aggcgcccaa agacuagcag ccuuaggcga caccgcgugg gacuucggau     1920

cuauaggcgg aguauucaac uccauaggac gcgcagucca ccaaguauuc gggggagcau     1980

ucagaacacu cuucggcggu augagcugga ucacacaagg ccucauggga gcacuccuac     2040

ucuggauggg cgucaacgca agggacagau ccaucgcccu cgcguuucua gcgaccgggg     2100

gagugcucgu auuccucgcc acgaacgucc acgcaugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10623
<211>  2455
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 2 opt

<400>  10623
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aacgcagcgc cggauccauc auguggcuag caucgcucgc aguaguaauc gcgugcgccg      120

gugcaaucac acgacgcggc agcgcauacu auauguaccu cgaccgaagu gaugccggga      180

aggcgaucuc auuugcaacc acgcuaggcg uaaauaaaug ccacguccag aucauggacc      240

ucggccacau gugcgacgcc accaugagcu acgaaugccc gaugcuggac gaaggcgugg      300

aacccgauga cgucgacugc uggugcaaca caacaagcac cuggguuguc uacggcacgu      360

gccaccacaa gaaaggcgaa gcaagaaggu cacgacgagc aguaacgcua ccaagccaca      420

gcacacgcaa acuacaaacc agaucacaaa cauggcucga gagccgcgaa uacaccaaac      480

accuuauaaa aguagaaaac uggauauuca gaaacccggg auucgcccua guagcaguag      540

caauagcaug gcuacugggc ucguccacga gucaaaaagu aauauaccua guaaugauac      600

uacugauagc acccgccuac ucaauccggu gcaucggagu auccaacagg gacuucguag      660

aagggauguc aggcgggaca uggguagacg ucguccuaga gcacggcgga ugcgucacgg      720

uaauggccca agacaaaccc acaguagaua ucgagcuagu aaccacgacc guaagcaaca      780

uggccgaagu cagaucguac ugcuacgaag ccagcaucag cgacauggcc uccgacagcc      840

gcugcccaac ccaaggggaa gccuaccuag acaaacaauc cgacacacaa uacguaugca      900

aaaggacccu aguagauaga ggguggggaa acggaugugg ucucuucggc aaaggcuccc      960

ucguaacaug cgccaaauuc acaugcagca aaaagaugac aggcaaguca auacagccag     1020

aaaaccucga guacagaauc augcucucag uccacgguuc acaacacagc ggaaugaucg     1080

gcuacgaaac ggaugaagac agagcaaaag uggaaguaac accuaacagc ccacgcgcag     1140

aagcgacacu aggaggauuc gggagucuag gauuggacug cgaaccaaga acaggccuag     1200

acuucagcga ccucuacuac cucacaauga acaacaaaca cuggcuaguc cacaaggaau     1260

gguuccacga cauaccacua ccauggcacg cgggcgccga caccggaaca ccgcacugga     1320

acaacaaaga agcccucguc gaguucaaag acgcgcacgc aaaacgacaa acgguaguag     1380

uccugggcuc acaggaaggc gcaguacaca cagcgcuagc cggagcccua gaggcagaaa     1440

uggacggcgc aaaaggccgc cuguucagcg gacaccuaaa augcagacuc aaaauggaca     1500

aacuacgccu aaaaggcgug ucauacagcc ucugcacggc cgccuuuacg uucacaaaag     1560

ugccagcaga aacacugcac ggcacaguua caguagaagu acaguaugcc ggaaccgacg     1620

gcccaugcaa aaucccugug cagauggccg uggacaugca aacacugaca ccgguaggac     1680

gacuaauaac cgcaaaucca gugauaacgg aaucaacaga aaacuccaaa augaugcugg     1740

aacucgaccc accauucggc gacagcuaca ucgugauagg cguaggagac aagaaaauaa     1800

cacaccacug gcaccggagu ggaagcacgc ugggaaaagc auucagcaca acgcucaaag     1860

gcgcccagag acucgcggca cucggcgaca ccgcauggga cuucggcuca aucggcgggg     1920

ucuuuaacag caucggacgc gcagugcacc aagucuucgg aggcgcguuc agaacccucu     1980

ucggaggcau guccuggaua acccaaggcc ucaugggcgc acugcuacua uggauggggg     2040

ucaacgcgag agacagaagc auagccuuag ccuuccuggc caccggagga guacugguau     2100

uccuagcaac aaacgugcac gcgugaggac uagugcauca cauuuaaaag caucucagcc     2160

uaccaugaga auaagagaaa gaaaaugaag aucaauagcu uauucaucuc uuuuucuuuu     2220

ucguuggugu aaagccaaca cccugucuaa aaaacauaaa uuucuuuaau cauuuugccu     2280

cuuuucucug ugcuucaauu aauaaaaaau ggaaagaacc uagaucuaaa aaaaaaaaaa     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa augcaucccc     2400

cccccccccc cccccccccc cccccccaaa ggcucuuuuc agagccacca gaauu          2455


<210>  10624
<211>  2467
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 2 opt

<400>  10624
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

aacggagcgc aggcuccaua auguggcuag caagccuagc cgucgugauc gcgugugcag      120

gcgccgucac ccggagaggc ucggcguacu acauguaccu cgaccgaaac gacgcaggag      180

aggcaaucuc auuccccaca acacucggaa ugaacaaaug uuacauccaa auaauggacc      240

uaggccacau gugcgacgca acaaugagcu acgagugccc aaugcucgac gagggaguag      300

aaccagacga cgucgauugc uggugcaaca caaccagcac cugggucguc uacggaaccu      360

gccaccacaa gaaaggcgaa gcacgccgaa gcaggcgcgc agucacucua ccgagucaua      420

gcacgcgcaa acuccaaaca cgcagccaaa ccuggcucga gagcagagaa uacacaaagc      480

accucauccg gguagagaac uggaucuuca ggaacccagg guucgcccua gccgcagcag      540

caauagcaug gcuccuugga agcagcaccu cacagaaagu aauauaccua guaaugauuc      600

uccucaucgc cccagcguac agcauaagau gcaucggagu cuccaauaga gacuucguag      660

aaggcauguc aggaggcaca uggguagacg uaguccuaga acacggcgga ugcgugaccg      720

uaauggcaca agacaaacca acgguggaua uagagcuggu cacaaccaca guaagcaaca      780

uggcggaagu ccggagcuac ugcuacgagg caucaaucag cgacauggca agcgacaguc      840

gaugccccac ccaaggcgaa gccuaccucg acaaacaaag cgacacgcaa uacguaugca      900

aacgcacacu cgucgacagg ggguggggaa acggcugcgg acucuucggc aaaggcucac      960

ucguuaccug cgcaaaauuc gcgugcucca agaaaaugac cggcaagucc auccaaccug     1020

aaaaccugga auaccgaauc augcuaucgg uccacggcag ccaacacagc ggaaugauag     1080

ugaacgauac aggucacgaa acagacgaga acagagcaaa aguagaaaua acuccaaacu     1140

caccgcgagc agaagccacc cugggcggcu ucggaagccu aggccucgac ugcgaacccc     1200

gcaccgggcu agacuuuagc gauuuguacu accucaccau gaacaacaaa cacuggcucg     1260

uccacaaaga augguuccac gacauacccc ucccauggca cgcgggcgca gacaccggaa     1320

ccccacacug gaacaacaaa gaagccuuag uagaguucaa agacgcacac gcaaagcgac     1380

aaaccguagu cgugcucggc ucccaagaag gagccguaca caccgcacuc gccggggcac     1440

uggaagcaga aauggacggc gcaaaaggaa gacucucauc aggacaccuu aaaugccgac     1500

ucaaaaugga caaacuaaga uuaaagggag ucagcuacag ucucugcacg gcggccuuua     1560

ccuucacaaa aauccccgcc gaaacacuac acggaacagu caccguggaa guacaauacg     1620

cgggaacaga uggaccaugc aaagugccag cccaaauggc agucgauaug caaacccuaa     1680

cccccguagg acgauugaua acagccaacc cagugauaac agaaagcacc gaaaacagca     1740

aaaugaugcu agaacuagau cccccauucg gagacagcua cauagucauc gggguaggag     1800

aaaagaaaau aacccaccac uggcaccgaa gcggcagcac acugggcaaa gccuuuucua     1860

cgacgcucaa aggagcacaa cgacucgcag cucucggaga cacagccugg gacuucggca     1920

guauaggagg cguauucaac ucgauaggga gagcagucca ccaaguauuc ggaggagcau     1980

ucagaacacu guucggaggc augagcugga uaacacaagg acuuaugggc gcacuccuac     2040

uguggauggg agucaacgcu agagacagau caauagcccu agccuucuug gccaccggcg     2100

gaguacucgu auuccuugca acaaacgugc acgcgugagg acuagugcau cacauuuaaa     2160

agcaucucag ccuaccauga gaauaagaga aagaaaauga agaucaauag cuuauucauc     2220

ucuuuuucuu uuucguuggu guaaagccaa cacccugucu aaaaaacaua aauuucuuua     2280

aucauuuugc cucuuuucuc ugugcuucaa uuaauaaaaa auggaaagaa ccuagaucua     2340

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2400

aaaugcaucc cccccccccc cccccccccc ccccccccca aaggcucuuu ucagagccac     2460

cagaauu                                                               2467


<210>  10625
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10625
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacau       60

ccgugggaau agucggacua uuacucacca ccgcaauggc cgcagucacc cucccaaguc      120

acucuacccg caaacuccaa acccgaucgc agaccuggcu cgaaagccgc gaauacacaa      180

aacaccucau ccgagucgaa aacuggaucu uccgaaaccc aggauucgcc cucgccgccg      240

ccgcaaucgc cuggcuccuc gggagcucua caucacaaaa agucauauac cucguaauga      300

uacuacucau agcccccgcc uauuccaucc gcuguauagg aguaucaaac cgagacuucg      360

uagaaggcau gagcggcggc accuggguag acaucguacu cgaacacgga ggcugcguaa      420

ccgucauggc acaagacaaa cccaccguag acauagaacu cgucacaaca accguaucaa      480

acauggcgga aguccgcucc uacugcuacg aagccucaau cagcgacaug gccuccgacu      540

cacgcugccc aacacagggc gaggccuacc uagacaaaca aagcgacacc caauacguau      600

gcaaacgcac acucgucgac cgaggcuggg gaaacggcug cggccucuuc ggaaaaggaa      660

gccucguaac cugcgccaaa uucgccugcu ccaagaaaau gacuggaaaa uccauacaac      720

cggaaaaccu cgaauacaga aucaugcuca gcguccacgg cucacaacac agcggcauga      780

ucgugaacga caccggacac gaaaccgacg aaaaccgcgc aaaaguagaa auaacuccaa      840

acucaccacg cgcugaggcc acacuaggcg gcuucggcuc ccuagggcuc gacugcgaac      900

caagaaccgg ucucgacuuc ucagaccucu acuaccucac aaugaacaac aaacacuggc      960

uuguccacaa agaaugguuc cacgacauac cccuccccug gcacgccggc gccgacacag     1020

gcacacccca uuggaacaac aaagaagccc ucguggaguu caaagacgcg cacgcaaaac     1080

ggcaaaccgu aguagugcua gggucccaag aaggagccgu ccacacggcc cucgcuggag     1140

cccucgaagc agaaauggac ggcgcuaaag gccgacuguc cucaggacac cucaaaugcc     1200

gccucaaaau ggacaaacua cgccucaaag guguuuccua cucacuaugc accgccgccu     1260

ucacguucac caaaauccca gccgaaaccc uccacggaac agucaccguc gaagugcaau     1320

acgccggcac cgacggcccc ugcaaagucc cagcccaaau ggcaguggac augcaaaccc     1380

uaaccccagu cggccgccua auuaccgcca accccgucau caccgaaucc acagaaaaca     1440

gcaaaaugau gcucgaacuc gacccacccu ucggcgacuc guacaucgug auaggcgucg     1500

gagaaaagaa aaucacacac cacuggcacc gcagcggcuc aacaauaggc aaagcguucg     1560

aagccaccgu ccgcggcgcc aaacgcaugg ccgugcucgg cgacacagcc ugggacuucg     1620

gauccgucgg uggugcccua aauagcuugg gcaagggaau ccaccaaauc uucggagccg     1680

ccuucaaaag ccuuuucgga gggauguccu gguucuccca gauccucaua ggaacccuac     1740

uaauguggcu aggccuaaac acgaagaacg gcucaauaag ccucaugugc cucgcacuag     1800

gcggaguacu aaucuuccug ucgacugccg uauccgcaug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10626
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10626
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacca       60

gcgucggaau agucggccuc cuccucacga cagcgauggc agccgugaca cuccccuccc      120

acucgacaag aaaacuacaa acacgcuccc aaacguggcu cgaauccaga gaguacacua      180

aacaccugau ccgugucgaa aacuggauau uccgcaaccc aggcuucgcc cuggcagccg      240

ccgcaaucgc cuggcuacuc ggcagcagca ccucacaaaa agucaucuau cuaguaauga      300

uacuccugau cgcgccagcu uacuccaucc gcugcauagg aguauccaac agagacuucg      360

ucgaaggcau gagcggaggc acaugggucg acgucguccu agaacacgga ggaugcguca      420

ccguaauggc acaagauaaa cccacggucg acaucgagcu aguuaccaca acagucagca      480

acauggccga aguccgcagu uacugcuacg aagccagcau aagcgacaug gccucagaca      540

gcagaugccc aacccaaggc gaagccuacc ucgacaaaca gucagacacg caauacguau      600

gcaaaagaac ccucguagau cgaggcuggg gcaacggaug uggacucuuc gggaaaggca      660

gccucguaac cugcgccaaa uucgcgugca guaagaaaau gacaggcaaa uccauccaac      720

cagaaaaccu cgaauaccgu auuaugcucu ccguccacgg cagccagcac uccggcauga      780

ucguaaacga caccggacac gagaccgacg aaaaccgagc caaagucgaa aucaccccca      840

acucaccccg cgcagaagcc acacucggcg gcuucggcuc ccucggacug gacugcgaac      900

caaggacagg ccuagacuuc ucagaccuau acuaccucac aaugaacaac aaacacuggc      960

ucgugcacaa agaaugguuc cacgacaucc cccuccccug gcacgccgga gcagacaccg     1020

gaacacccca cuggaacaac aaagaggccc uuguagaguu caaagaugca cacgccaagc     1080

gccaaaccgu aguuguacug ggaagccaag aaggcgcagu acacaccgca cuagcaggag     1140

cacuagaagc cgaaauggac ggcgccaaag gccgccuauc cagcggccac cuaaaaugcc     1200

gccucaaaau ggacaagcuc aggcugaaag gcgucagcua cucccucugc accgcagcau     1260

ucaccuucac caaaauaccu gcagaaaccc uccacggcac cgucaccguc gaaguacaau     1320

acgcaggcac ggacggccca ugcaaaguac ccgcccaaau ggccguggac augcaaaccc     1380

ucacaccagu aggccgacuc aucacagcca auccaguaau cacggaaagc acagaaaaca     1440

guaaaaugau gcuagaacuc gacccacccu ucggagacuc uuacaucguc auaggaguag     1500

gcgaaaagaa aaucacccac cacuggcauc gcuccggcuc aaccaucggc aaagcauucg     1560

aagccaccgu acgcggcgcc aaacguaugg cuguccuagg agacaccgca ugggacuucg     1620

gaucggucgg cggcgcccuc aacagccuag gaaaaggcau ccaccaaauc uuuggagcgg     1680

cguucaaauc ccucuucgga ggaaugucgu gguucuccca aauacuaauc gguacgcuac     1740

uaauguggcu cggccucaac gccaaaaacg gaucaauuag ccucaugugc cuagcacuag     1800

gcggaguccu aaucuuccua agcacagcgg uaagcgccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10627
<211>  2158
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10627
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaccu       60

ccaucggaau aauuggccua cuacucacaa ccgcaauggc ggccguaacc cuucccucac      120

acuccacucg uaaacugcaa acccgcuccc agacauggcu cgaaucgaga gaauacacaa      180

aacaccuaau caaagucgaa aacuggaucu uccgcaaccc cggauucgcc cuagucgccg      240

ucgcaaucgc auggcuccuc ggcuccucaa ccagccaaaa aguuaucuac cucguaauga      300

uccuccucau cgcacccgca uacucaauac gcugcaucgg cgucucuaac cgcgacuuug      360

uagaaggaau gucaggcggc accugggucg acgucguacu cgaacacggg ggaugcguca      420

ccgucauggc ccaagacaaa ccuacagucg acaucgagcu agucacgacc acagucucaa      480

acauggcaga gguaagaagc uacugcuacg aagccuccau cuccgauaug gccuccgacu      540

cccgaugccc cacacagggc gaagcauacc uagacaaaca auccgacacc caauacgucu      600

gcaaacgcac acucguagac cguggcuggg ggaacggcug cggacuauuc ggaaagggcu      660

cccucgucac augcgcgaaa uuuaccugcu ccaagaaaau gaccggaaaa agcauacagc      720

ccgaaaaccu cgaauaccgc aucaugcucu ccgugcacgg cagucaacac ucaggcauga      780

uaggcuacga aacagaugaa gaccgcgcaa aggucgaagu caccccaaac ucccccagag      840

ccgaagccac ccucgggggc uucggcagcc ucggacuaga cugcgaaccu agaaccggcc      900

uagacuucag cgaccucuac uaccuaacga ugaacaacaa acacuggcuu guacauaaag      960

aaugguucca cgacaucccc cugcccuggc acgccggagc cgacacagga acaccgcacu     1020

ggaacaacaa agaagcacuc guugaguuca aagacgccca cgcaaaacgc caaacagucg     1080

ucguacuagg aagccaagaa ggcgccgucc acaccgcacu agcaggagca uuagaagccg     1140

aaauggacgg cgcaaaaggc cgccucuucu ccggccaccu caaaugccga cuaaaaaugg     1200

auaaacuccg ccucaaaggc gucagcuacu cacucugcac cgccgccuuc acauucacca     1260

aaguccccgc cgaaacacua cacggcaccg uaacugucga gguacaauac gccggaacgg     1320

acggcccuug caagauaccc guacaaaugg caguggacau gcaaacacuc acccccgucg     1380

gcagacuaau aacagccaac ccgguaauaa ccgaaagcac cgagaacucu aaaaugaugc     1440

uagaacucga cccacccuuc ggcgacagcu acaucgucau cggcgucggc gacaagaaga     1500

ucacacacca cuggcacaga ucagguucca caaucggcaa ggcauucgaa gccacggucc     1560

gcggcgcaaa acgaauggcc guccuaggcg auaccgccug ggacuucggc uccgucggag     1620

gagucuucaa cucccucgga aaaggaaucc accaaaucuu uggagccgcc uucaaaucgc     1680

uauucggcgg caugagcugg uucagccaga uccuaauagg uacacugcuc guauggcucg     1740

gcuuaaauac caaaaacgga agcaucuccc uaacgugccu cgcccucggc ggcgucauga     1800

ucuuccucuc cacagccgug agcgcaugag gacuagugca ucacauuuaa aagcaucuca     1860

gccuaccaug agaauaagag aaagaaaaug aagaucaaua gcuuauucau cucuuuuucu     1920

uuuucguugg uguaaagcca acacccuguc uaaaaaacau aaauuucuuu aaucauuuug     1980

ccucuuuucu cugugcuuca auuaauaaaa aauggaaaga accuagaucu aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2100

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2158


<210>  10628
<211>  2170
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10628
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugacaa       60

gcguaggcau agucgggcuc cuacuuacaa cagccauggc agccguaaca uuaccaucac      120

acuccacccg caaacuacag accagauccc aaaccuggcu cgaaagccgc gaauacacaa      180

aacaccucau acgcgucgaa aacuggaucu ucagaaaccc cggguuugcc cuagcagccg      240

cagcaauagc cuggcugcug ggaagcucca caucacaaaa ggucaucuac cuaguaauga      300

uccugcucau agccccagcc uacuccaucc gcugcauagg cgucagcaac cgcgacuucg      360

uagaagggau gucaggcggu acauggguag acgucguacu cgaacacgga ggaugcguca      420

ccguaauggc gcaggacaaa ccaacaguag acaucgaacu cgucacaacc accgucucga      480

acauggccga agugcgaagc uacugcuaug aagcauccau cucagacaug gcguccgaca      540

gccgaugccc cacccaagga gaggcauauc ucgacaaaca aagcgacacc caauaugugu      600

gcaaacgcac ccucguggac cgcggauggg gaaacggaug cggccuguuc ggaaaaggcu      660

cacucguaac augcgcaaaa uucgcaugca gcaagaaaau gaccggaaaa agcauacagc      720

cugaaaaccu agaauacaga auaaugcuga gcguccacgg cucacaacac uccggcauga      780

uaguaaacga cacaggccac gaaaccgacg aaaacagagc caaaguagaa aucacaccaa      840

auuccccacg agcagaagcc acacucggcg gcuucggcag ccuaggccua gacugcgaac      900

cacggacagg acuagacuuc agcgaccucu acuaccucac aaugaacaac aaacacuggc      960

ugguccacaa agaaugguuc cacgauauac cccuccccug gcacgccgga gcagacaccg     1020

gcaccccaca cuggaacaac aaagaagcac uggucgaguu caaagacgcu cacgcgaaac     1080

gccagacagu cgucguacug ggcagccaag aaggcgccgu ccacaccgcu cucgccggcg     1140

cacuagaagc ggaaauggac ggggcaaaag gccggcuauc aagcggccac cugaaaugcc     1200

gacucaaaau ggacaaacua cgacugaagg gcgucagcua cagucuaugc acagccgcau     1260

ucaccuucac aaaaaucccc gcagaaaccc uacacggcac agucaccguc gaagugcaau     1320

acgccggcac agacggaccc ugcaaagucc ccgcucagau ggccgucgac augcaaacau     1380

uaacccccgu cggacgacua auaaccgcaa acccaguaau caccgaaucc acagagaacu     1440

ccaaaaugau gcucgaacuc gaucccccau ucggcgacag cuacauagua aucggcguag     1500

gagaaaagaa aauaacgcac cacuggcacc gaagcggcuc caccauaggc aaagcauucg     1560

aagcgaccgu ccgcggagcc aaacgcaugg cagucuuagg cgacaccgca ugggacuucg     1620

gcuccgucgg cggcgcccua aacagccucg gaaaaggaau ccaccaaauc uucggcgccg     1680

cauucaaaag ccuauucggg ggcaugucau gguucuccca aauccugaua ggaacccuac     1740

ucauguggcu agggcugaac accaaaaacg gcagcauaag ccuaaugugc cuagcacuag     1800

gcggaguccu cauauuucuc ucaacagccg ucucggccug aggacuagug caucacauuu     1860

aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa uagcuuauuc     1920

aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac auaaauuucu     1980

uuaaucauuu ugccucuuuu cucugugcuu caauuaauaa aaaauggaaa gaaccuagau     2040

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2160

caccagaauu                                                            2170


<210>  10629
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10629
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

uaucaggcgu cccuguccuc ggcuucuuca ucaucgccgu acuaaugucc gcccaagagu      120

caugggccgc agugacccuc ccaagccaca gcacgcgcaa acuacaaacc cgcagccaga      180

ccuggcucga aagccgagaa uacaccaaac accuaauaag agucgagaac uggaucuucc      240

gcaaccccgg auucgcgcuc gcggccgccg ccaucgcaug gcuccuaggc agcuccaccu      300

cacaaaaagu cauauaccuc gugaugaucc uccuuaucgc ccccgccuac uccauccgau      360

gcaucggagu cagcaaccga gacuucgucg aaggaauguc aggcggcaca ugggucgaca      420

uaguccucga acacggcgga ugcgucaccg uaauggccca agacaaaccc acagucgaca      480

ucgagcuagu cacaaccaca guaucaaaca uggcagaagu aagaagcuac ugcuacgaag      540

ccuccaucuc agacauggcc ucggacucac gaugccccac gcagggagaa gccuaccugg      600

acaaacaguc ugacacacaa uacgucugca aaagaacccu cguagaccgc ggauggggca      660

acggcugcgg acuauucgga aagggcuccc uaguaaccug cgcaaaauuc gcaugcucga      720

agaaaaugac cggcaaauca auucaacccg agaacuuaga auaccgcaua augcucucag      780

uacacggcuc acaacauagc gggaugaucg uuaacgacac cggccacgaa acagacgaaa      840

accgagcgaa agucgaaaua acaccaaaca gcccacgcgc ggaagccacc cugggcggcu      900

ucgggucccu cggcuuagac ugcgaaccgc gaacaggacu agacuuuagu gaccucuacu      960

accucaccau gaacaacaaa cacugguuag uacauaaaga augguuccac gacaucccac     1020

ugcccuggca cgccggcgcc gacaccggca caccccacug gaacaauaaa gaagcccucg     1080

uagaguucaa agacgcccac gcgaaacgac aaacagucgu cguacuaggc agccaagaag     1140

gcgcugucca caccgcucuc gccggagcac ucgaagccga aauggaugga gcgaaaggac     1200

gacuaagcuc cggccaccuc aagugccgac ucaaaaugga caagcuccgc cugaaaggcg     1260

ucaguuacuc ccuuugcacu gcagcauuua ccuuuacgaa aauacccgcc gaaacucuac     1320

acggaaccgu cacaguagaa guccaauacg ccggcacaga cggaccaugc aaggucccag     1380

cccaaauggc cgucgacaug caaacacuua ccccaguagg aagacuaaua acagccaauc     1440

ccguaaucac agaaucuacc gagaacucaa aaaugaugcu cgagcucgau cccccauuug     1500

gcgacagcua caucgucaua ggggucggcg aaaagaaaau cacacaccac uggcaccgca     1560

gcggcagcac aauaggcaaa gcauucgaag ccacagucag aggcgcaaaa cguauggcgg     1620

uacugggcga cacagccugg gacuucggaa guguaggcgg agcacuaaac ucguuaggca     1680

aaggcauaca ccaaauauuc ggagcagccu ucaaaagccu guucggcgga augucauggu     1740

ucucgcaaau ccuaaucggc acucuccuca ugugguuagg ccucaacacc aaaaacggga     1800

gcaucagccu aaugugccuc gcacucggag gagugcucau cuuccuaagc acggcagucu     1860

ccgccugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10630
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10630
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcca       60

ucagcggcgu ccccguacuc ggauucuuca ucaucgccgu acuaaugucc gcccaagaaa      120

gcugggcagc cgugaccuua ccaucacacu caacuagaaa acuccaaaca cgcucccaaa      180

ccuggcucga aucuagagag uacaccaaac accucauccg aguagaaaac uggaucuucc      240

gaaacccagg auucgcacuu gccgcagccg cgaucgcaug gcugcucgga agcucuaccu      300

cacaaaaagu aauauaccua gucaugauuc uccuuaucgc ccccgcauac agcauacgcu      360

gcaucggagu cuccaacaga gacuucgucg aaggcaugag cggcggcaca uggguagacg      420

uaguccucga acacggagga ugcgucaccg uaauggccca agacaaaccc accguagaca      480

uagaacuggu cacaacaacc gucucaaaca uggccgaggu aaggagcuac ugcuacgaag      540

ccuccaucuc cgacauggca agcgacaguc gcugcccaac ccaaggagaa gccuaccuag      600

acaagcaaag ugacacccaa uacguaugca aacgaacccu cgucgaccga ggauggggaa      660

acgggugcgg ccuauucgga aaaggaagcc ucgucacgug cgcaaaauuc gcaugcagca      720

agaaaaugac aggcaaaagc auccaacccg aaaaccucga guacagaaua augcuaagcg      780

ugcacggcuc acaacacucc ggcaugaucg uaaacgacac aggacacgaa accgacgaga      840

accgagccaa agucgaaauc acccccaacu caccacgcgc cgaagcaacc cucggcggau      900

uugguucccu cggacuagac ugcgaaccaa gaaccggacu cgacuucucc gacuuauacu      960

accuaaccau gaacaacaaa cacuggcuag uacauaaaga augguuccac gacaucccac     1020

uacccuggca cgcgggcgcg gacaccggca ccccacacug gaacaacaaa gaagcacucg     1080

uagaauuuaa agacgcacac gccaaacgcc aaacaguggu agugcuagga ucccaggaag     1140

gagccgucca caccgcacuc gcaggggccc uugaagccga aauggacgga gcgaaaggcc     1200

gccuuucgag cggacaccuc aagugccgcc uaaagaugga caaacuccga cucaagggag     1260

uaagcuacuc ccucugcaca gcagcauuca cauucacaaa aauacccgcc gagacacuuc     1320

acggcaccgu caccgucgaa guccaguaug ccggaaccga cggacccugc aaaguaccag     1380

cucaaauggc cguagacaug caaacccuaa cgcccgucgg acgacuaaua acagcgaacc     1440

cggucaucac cgaaucaacc gaaaacagca aaaugaugcu cgaacuagau cccccauucg     1500

gagacagcua cauaguaauc ggcguaggag aaaagaaaau cacgcaccac uggcaccgcu     1560

caggaucaac cauaggaaaa gcguucgaag caaccgugcg cggcgcuaaa cgcauggcag     1620

uccucggcga caccgccugg gacuuuggau cagucggagg agcccucaac agccucggca     1680

aaggcauaca ccaaaucuuc ggagcagccu uuaaaucccu cuucggaggc augucguggu     1740

ucagccaaau acuaaucggg acccuacuaa uguggcuagg acucaacgcc aaaaacggca     1800

gcauaucccu caugugccua gcccucggcg gaguccucau auuucuauca acagccgucu     1860

cagcuugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10631
<211>  2185
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10631
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcga       60

uauccggagu cccaguccuc ggcuucuuca ucaucgcagu ccugauguca gcgcaagaaa      120

gcugggcagc ggucacacua cccagccacu ccacacgcaa acuccaaaca cgcagccaga      180

cauggcuaga aucccgcgaa uacacaaaac accucaucaa gguagaaaac uggaucuucc      240

gaaaccccgg auucgcgcuc gucgcuguag caaucgcaug gcuacucggc agcucgacau      300

cgcaaaaagu caucuaccua gucaugaucc uacucaucgc acccgccuac uccauaagau      360

gcaucggcgu cuccaaccga gacuucguag aaggaauguc aggcggcacc ugggucgacg      420

ucguccuaga gcacggaggc ugcguaaccg ucauggcaca ggacaaacca acagucgaca      480

uagaacucgu aacaaccacc guaucaaaca uggccgaagu ccguucauac uguuacgaag      540

ccucaauaag ugacauggcc ucagacagcc gaugccccac ccagggggag gcauaccugg      600

acaagcaauc agacacccaa uacgucugca aaagaacgcu cguagaccgc ggcuggggua      660

acggcugcgg gcuauucggc aaaggcuccc ucgucacgug cgccaaauuc acgugcagca      720

agaaaaugac cggaaaaagc auccaaccug aaaaccucga auaccgaaua augcuguccg      780

uccacggcag ccaacacagc ggcaugaucg gcuacgaaac cgacgaagac cgcgcaaaag      840

ucgaagucac acccaacuca ccacgcgcgg aagccacacu gggaggauuc ggaagccuag      900

gacuagacug cgaaccacga acaggacucg acuucagcga ccuuuacuac cucaccauga      960

acaacaaaca cugguuaguc cacaaagaau gguuccacga caucccccuc cccuggcacg     1020

ccggagccga cacaggaacg ccccacugga acaacaaaga agcccucguc gaguucaaag     1080

acgcgcacgc aaaaaggcaa accgucgucg uucuaggauc acaagaaggc gcgguacaca     1140

ccgcgcuagc cggcgcccua gaagccgaaa uggacggugc caaaggucgc cucuucucag     1200

gacaccucaa augccggcug aaaauggaca aacuccgccu aaaaggcgua uccuacagcc     1260

ucugcaccgc agccuucacc uucacaaaag ucccugccga aacccugcac ggcaccguca     1320

ccgucgaagu ccaauacgcc ggaacggaug gccccuguaa aauccccgua caaauggccg     1380

uagacaugca aacccucacg cccguaggcc gacucaucac cgccaaccca gucauaacag     1440

aauccaccga aaauagcaag augaugcuag aacucgaucc cccauucgga gacucauaca     1500

uagucaucgg agucggcgac aaaaagauaa cacaccacug gcaccgcuca ggcucaacca     1560

ucggcaaagc cuucgaagcc accguccgcg gcgcaaaaag aauggcaguc cucggagaca     1620

ccgcauggga cuucggcucc guaggaggcg uauucaauag ccuaggcaag ggcauucacc     1680

aaauauucgg ggcagccuuc aagagcuuau ucggcgggau gucaugguuc ucccaaauac     1740

ucaucggaac acuccuagug uggcucggcc ucaacacaaa gaacggcuca aucagccuua     1800

ccugccucgc acucggaggc gugaugauau uccuauccac agccguaucc gccugaggac     1860

uagugcauca cauuuaaaag caucucagcc uaccaugaga auaagagaaa gaaaaugaag     1920

aucaauagcu uauucaucuc uuuuucuuuu ucguuggugu aaagccaaca cccugucuaa     1980

aaaacauaaa uuucuuuaau cauuuugccu cuuuucucug ugcuucaauu aauaaaaaau     2040

ggaaagaacc uagaucuaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa augcaucccc cccccccccc cccccccccc cccccccaaa     2160

ggcucuuuuc agagccacca gaauu                                           2185


<210>  10632
<211>  2197
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10632
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggcaa       60

uauccggcgu gcccguacuc ggauucuuca ucaucgccgu ccucauguca gcgcaagaau      120

ccugggcagc cguaacacuc cccucccaca gcacccgcaa acuacaaaca agaagccaaa      180

ccuggcuaga aucacgagaa uacaccaagc accuuauaag agucgaaaac uggauauucc      240

gaaacccagg auucgcacug gcggccgcag caauagcaug gcuccuaggc ucgaguacaa      300

gccaaaaagu aaucuaccuc guaaugaucc uacuaaucgc acccgcguac ucaaucagau      360

gcaucggcgu cagcaacaga gacuucguag aaggaauguc cggagggacu ugggucgacg      420

uaguacuaga acacggcggc ugcgucacag uaauggcaca agacaaacca accgucgaca      480

uagagcuagu gaccacaacc gucagcaaca uggcggaagu acgcagcuac ugcuacgaag      540

caagcauauc ggacauggca agcgacagcc gcugcccaac acagggcgaa gcauaccuag      600

acaaacaauc cgacacccaa uacguuugca agcgaacacu cgucgaccgc ggcuggggca      660

acggcugcgg ccuauucggc aaaggcuccc uaguaacaug cgcaaaauuc gcaugcucua      720

agaagaugac aggcaagagc auacaaccag aaaaccucga auacaggauc augcuaucgg      780

uccacgguuc acaacauuca ggcaugaucg ucaaugacac gggacacgaa accgacgaaa      840

accgcgccaa gguggaaaua acgcccaaca guccccgcgc cgaggcaacc cugggcgguu      900

ucggcagccu cggccucgac ugcgaaccaa gaaccggacu cgacuucagc gaccuauacu      960

accuaacuau gaacaacaag cacuggcuug uccacaaaga gugguuccac gacauacccc     1020

uccccuggca cgcaggagca gacaccggaa ccccacacug gaacaacaaa gaagcacucg     1080

uagaguucaa agacgcccac gcgaaaagac aaacagucgu gguacugggg agccaagaag     1140

gugccgucca cacagcccua gccggagcac ucgaagcaga aauggacggc gcaaaagggc     1200

gccucucauc aggccaccua aagugccgac uaaaaaugga caaacuccgc cuaaaaggcg     1260

uaucuuacuc ccuaugcacc gccgcauuca ccuuuacaaa gauaccggcc gaaacccucc     1320

acggcaccgu aacaguagaa guccaauacg ccggcaccga cggccccugc aaaguccccg     1380

cccaaauggc aguagacaug caaacacuaa cacccgucgg ccgacucaua accgccaacc     1440

ccgucauaac agaaaguacu gaaaacucca aaaugaugcu cgaacucgac ccacccuucg     1500

gagacagcua cauagucaua ggcgucggcg agaaaaagau aacccaccac uggcaccgcu     1560

ccggcuccac aauaggcaag gccuucgaag ccaccguaag aggcgcaaaa cgcauggcag     1620

uccugggaga cacagcaugg gacuucggcu ccguuggagg ggcacuaaac ucacuaggga     1680

aaggcauaca ucaaauuuuc ggcgcagccu ucaaaagccu auucggcgga augagcuggu     1740

ucagccaaau acuaaucggc acacuacuua uguggcucgg ccucaacacg aaaaacggaa     1800

gcaucucccu aaugugccua gcacucggcg gcgugcuaau auuccuaagc acagcaguca     1860

gcgcaugagg acuagugcau cacauuuaaa agcaucucag ccuaccauga gaauaagaga     1920

aagaaaauga agaucaauag cuuauucauc ucuuuuucuu uuucguuggu guaaagccaa     1980

cacccugucu aaaaaacaua aauuucuuua aucauuuugc cucuuuucuc ugugcuucaa     2040

uuaauaaaaa auggaaagaa ccuagaucua aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaugcaucc cccccccccc cccccccccc     2160

ccccccccca aaggcucuuu ucagagccac cagaauu                              2197


<210>  10633
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10633
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggacauggau acuauuccuc guagcagcag cgacaagagu gcacagugca guaacccucc      120

ccucacacag cacgagaaaa cuacagacgc gcagccaaac cuggcucgaa ucacgagaau      180

acaccaaaca ccuaauacga gucgagaacu ggauauucag aaaccccggc uucgcccuag      240

cagcagcagc aaucgccugg cuccuaggca gcagcacauc acaaaaagug auauaccugg      300

uaaugauccu ucuuauagcc cccgccuaua guauaagaug caucggcguc agcaaucgcg      360

acuucgucga aggaaugucg ggaggaaccu gggucgacau aguucuagag cacggaggcu      420

gcgucaccgu aauggcacaa gacaaaccaa caguggacau cgaauuaguu accacaacgg      480

uaagcaacau ggccgaaguc agaagcuauu gcuacgaagc aagcauauca gacauggcaa      540

gcgacucccg augccccacc caaggcgagg cuuaccuaga caaacagagu gacacccagu      600

acgugugcaa aagaacccua gucgaccgug gcuggggcaa cggaugcgga cucuucggca      660

aagggagccu cguaacaugc gcaaaauucg cuugcucaaa gaaaaugacc ggcaaaagca      720

uccaaccaga aaaccuagaa uaccgaauca ugcucuccgu acacggauca caacacagcg      780

gaaugauagu gaacgacacc gggcacgaga cagacgagaa ccgcgccaaa guagaaauaa      840

cccccaacag ucccagagcc gaagcgacac uaggaggauu cggaucauua gggcuggacu      900

gcgagccacg aaccggccua gacuucucag accuauacua ccucacgaug aacaacaaac      960

acuggcucgu acacaaggaa ugguuccacg acauaccccu accauggcac gccggcgcag     1020

acacaggcac accccacugg aacaauaaag aagcccucgu cgaauuuaaa gacgcccacg     1080

ccaaacgcca aacaguggug guacucggcu cacaagaagg ugccguucac acggcccuag     1140

caggcgcccu ggaagccgaa auggacggag ccaaaggucg acuaagcagc ggacaucuaa     1200

aaugcagacu gaaaauggac aaacuccggc ucaaaggagu aucauacagc cuaugcaccg     1260

ccgccuucac guucacaaaa auaccagcag agacccucca cggcacaguc acgguagaag     1320

uccaauacgc cggcaccgac ggccccugca aagugccagc acaaauggca gucgacaugc     1380

aaacccugac acccgucggc aggcuaauca ccgcaaaccc cgucaucaca gagaguacag     1440

agaacagcaa aaugaugcuc gaacucgacc cacccuucgg agacucguac aucgucauag     1500

gcgucggcga aaagaaaauc acccaucacu ggcauagaag cggaagcacc auaggcaaag     1560

ccuucgaagc caccguacgc ggggccaaac gcauggccgu ccucggagac acagcguggg     1620

acuucggcuc gguaggcggc gcacucaacu cacucggcaa aggcauacac caaaucuucg     1680

gcgcagccuu caaaucccua uucggcggga ugagcugguu cucgcaaaua cuaauaggca     1740

cccugcuaau guggcucgga cuaaacacca aaaacggcuc gaucucauua augugccuag     1800

cgcucggcgg aguacuaauc uuccuaucaa cagcaguauc agccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10634
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10634
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggaccuggau ccucuuccuc guagccgccg ccacacgcgu acacagcgca gucacacucc      120

cgagccacag caccagaaag uuacaaacac gcucucaaac guggcuagag ucccgagaau      180

acacaaaaca ccuaauacgc guagaaaacu ggaucuucag aaacccaggu uucgcccugg      240

cggcagccgc caucgcgugg cuacucggcu caagcaccag ccaaaaaguc aucuaccucg      300

ucaugauccu acucauagcc ccagccuacu ccauccgaug caucggaguc uccaauagag      360

acuucgugga gggaaugagc ggcgguaccu gggucgacgu uguccuagaa cacggcggcu      420

gcgucaccgu uauggcccaa gacaaaccca ccgucgacau agaauuaguc accacaacag      480

ucucaaacau ggccgaaguc cgaagcuacu guuacgaagc cuccaucucc gacauggcca      540

gcgacucacg gugcccgacc caaggcgagg cuuaccucga caaacaauca gacacacaau      600

acgucugcaa acgcacacuc gucgacagag gcuggggcaa cggcugcggc cuguucggca      660

aaggcucccu aguuaccugc gcuaaauucg caugcagcaa gaaaaugacc ggcaaaagca      720

uccagcccga aaaccuagaa uacagaauca ugcucucagu ccacggaagc caacacagcg      780

gcaugauagu caacgacacc ggccacgaaa ccgacgaaaa cagagccaaa gucgaaauca      840

cccccaacuc accccgcgcg gaggccacac uuggcggauu cggcucccua ggacuggacu      900

gcgaaccccg caccgggcua gacuucuccg accuauacua ccucacaaug aacaacaaac      960

acuggcuagu ccacaaagaa ugguuccacg acauaccauu acccuggcau gccggcgcag     1020

acacaggaac accccacugg aacaacaaag aagcacucgu cgaguucaaa gacgcccacg     1080

ccaaacgcca aaccgucguc gugcuaggau cccaagaagg ugccguacac accgcccuug     1140

caggggcucu cgaagccgaa auggacggcg ccaagggacg ccuuuccucc ggccaccuga     1200

aaugccggcu caaaauggac aaauuacgac ucaagggugu aucauacucc cucugcaccg     1260

cagcauucac cuucaccaaa auccccgccg aaacgcucca uggcaccguc acagucgaag     1320

uucaauacgc cggcaccgac ggacccugca aggugccggc gcaaauggcc gucgacaugc     1380

aaacccuaac gcccguaggg aggcucauca cugccaaccc aguaauaaca gaaagcacag     1440

aaaacuccaa aaugaugcuc gaacucgacc cgcccuucgg ggacucauac auaguaauug     1500

gcguaggcga aaagaaaaua acacaccacu ggcacagaag cggaucaaca auaggaaaag     1560

ccuucgaagc aacgguaaga ggggccaaaa gaauggccgu acucggagac accgccuggg     1620

acuucggcuc aguugggggu gcacuaaacu cccucggcaa agguauccac caaauauucg     1680

gcgcugcauu caaaucacuc uucggaggaa ugucaugguu cucccagaua cucauaggca     1740

cccugcucau guggcucggc cucaacgcga aaaacgguag cauuucacua augugccucg     1800

cauuaggcgg agugcucaua uuccuaucaa ccgcagucag cgccugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10635
<211>  2164
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10635
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggauu       60

ggaccuggau acucuuccua gucgcagcag caaccagagu acacucagcc guuacccucc      120

ccagccacuc aacaagaaag cugcaaacac gcagccaaac guggcuagaa ucccgcgaau      180

acacgaaaca ccucauaaag gucgaaaacu ggaucuucag aaacccaggc uucgcgcucg      240

ucgcggucgc caucgcaugg cuacucgggu ccagcacuuc ccaaaaaguc auauaccuag      300

ucaugauccu acucaucgca cccgcuuaca gcauccgcug caucggagua uccaaucgag      360

acuucguaga aggcaugagc ggaggcacau ggguagacgu aguccucgaa cacggcggau      420

gcgucaccgu aauggcgcaa gacaaaccca ccguagacau cgaacucgua acaacaaccg      480

ucucgaacau ggcagaaguc agaagcuacu gcuacgaagc aagcauaagc gacauggcga      540

gcgacucccg cugccccacc caaggggaag ccuaccugga caaacaaagc gacacccaau      600

acguaugcaa acgaacacuc guagauagag gcuggggcaa cggaugcggc cuauucggaa      660

agggaucccu agucacaugc gcaaaauuca caugcucaaa gaaaaugaca ggcaaaucaa      720

uacaacccga aaaccucgaa uaccgcauca ugcucagcgu ccacggauca caacacucgg      780

gaaugauagg auacgaaacg gacgaagacc gggcaaaagu agaggucaca ccaaacuccc      840

caagggcgga agccacccuc ggcgguuucg gcucacuggg gcuagacugc gaaccccgca      900

ccggacucga uuucucagac cuauacuacc ucacaaugaa caacaaacac uggcucgugc      960

acaaggaaug guuccacgac auaccccucc cguggcacgc cggcgcggac acaggaacac     1020

cccacuggaa caauaaagaa gcacucgucg aguucaaaga cgcacacgcc aaacgccaga     1080

ccguaguagu acucggauca caagaagggg ccguacacac ggcgcuggcc ggagcacuag     1140

aagcagaaau ggauggcgca aaaggccggc uauucagugg acaccuaaaa ugucgccuaa     1200

aaauggacaa acucagacuc aaaggagucu ccuacucccu augcacggcc gcguucacau     1260

ucacaaaagu ccccgcagaa acacuccacg gcacgguuac agucgaaguc caauacgcag     1320

gcaccgacgg ccccuguaaa auaccuguac aaauggccgu cgacaugcaa acacucacac     1380

ccgucggaag acugaucacu gcaaacccag ucauaacaga aaguacagaa aacagcaaaa     1440

ugaugcuaga acuugaccca cccuucggcg auucguacau cgucaucggc guaggagaca     1500

agaaaaucac ccaccacugg caccgaagcg gcagcaccau aggaaaagca uucgaagcaa     1560

caguacgcgg agccaaaaga auggccgucc uuggagacac agccugggac uucggguccg     1620

ucggaggcgu auucaacagc cugggaaaag gcauccacca gaucuucggc gcagcauuca     1680

aaucgcuauu cgguggaaug uccugguuca gccaaauccu aaucgggacc cucuuagugu     1740

ggcuaggacu aaacacgaaa aacggcagca ucagccuaac cugccuagcc cugggagggg     1800

ucaugaucuu ccucucaacu gcagucucag caugaggacu agugcaucac auuuaaaagc     1860

aucucagccu accaugagaa uaagagaaag aaaaugaaga ucaauagcuu auucaucucu     1920

uuuucuuuuu cguuggugua aagccaacac ccugucuaaa aaacauaaau uucuuuaauc     1980

auuuugccuc uuuucucugu gcuucaauua auaaaaaaug gaaagaaccu agaucuaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

ugcauccccc cccccccccc cccccccccc ccccccaaag gcucuuuuca gagccaccag     2160

aauu                                                                  2164


<210>  10636
<211>  2176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10636
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accauggacu       60

ggacguggau acucuuccuc guagcagcgg ccacaagagu ccacagcgca guaacacugc      120

ccucacacuc uacccgcaaa cugcaaacac gcagccaaac auggcuagaa ucacgcgagu      180

acacaaaaca ccugauccga gucgaaaacu ggauauucag aaacccagga uucgcccugg      240

cagcggccgc cauagccugg cuacucggaa gcucuaccuc acaaaaggua auauaccuag      300

uaaugauccu ccucaucgcc cccgccuacu caauacgcug caucggagua agcaaccgcg      360

acuucgucga aggaaugagc ggaggaaccu ggguggacgu cgucuuagag cacggaggau      420

gugucacggu aauggcccaa gacaaaccca ccgucgacau agaguugguc accacaacag      480

ucuccaacau ggccgagguc agaagcuacu guuacgaagc aaguaucuca gacauggcca      540

gcgacagccg cugcccaaca caaggagaag cauaccucga caagcaaagu gacacacaau      600

acgugugcaa gcgcacgcua guagaccgag gcuggggaaa cggcugcggc cucuucggaa      660

aaggcagccu cguaacaugc gccaaauucg ccugcuccaa aaagaugacc ggcaagucga      720

uccaacccga aaaccucgaa uacagaauca ugcuauccgu acacggaagc caacacucag      780

gcaugauagu caacgacacu ggacacgaaa ccgacgaaaa ccgcgccaaa guagaaauca      840

caccaaacuc accacgagca gaagcaaccu uaggaggauu cggcucccua ggacucgauu      900

gcgagcccag aacagggcug gacuucucag accuauacua ccuaacaaug aacaacaaac      960

acuggcuagu acacaaagaa ugguuucacg acauaccacu accauggcac gcuggugcag     1020

acacgggaac gccccacugg aacaacaaag aagcacucgu agaguucaaa gacgcacacg     1080

caaagagaca aacaguuguc guccuaggaa gccaagaagg cgccguacac acggcccucg     1140

ccggagcucu cgaagccgaa auggacggag cuaagggaag acucagcagc ggccaccuaa     1200

aaugccgacu aaaaauggac aaacuccgac ucaagggagu aagcuacuca cuaugcacgg     1260

ccgccuucac auucacaaaa auacccgccg agacccucca cggaaccguc accgucgagg     1320

uacaguacgc aggaaccgac ggacccugca aaguacccgc gcagauggca guagacaugc     1380

aaacacucac ccccguggga cgccucauaa ccgcgaaccc agucauaacc gaguccacgg     1440

aaaacagcaa aaugaugcua gaacuugacc caccauucgg agacuccuac aucguaauag     1500

gcgucggcga aaagaaaauc acacaccacu ggcaccgauc aggcucgacc aucggaaaag     1560

ccuucgaagc aacaguacgc ggcgccaaac gcauggcagu ccucggagac acggccuggg     1620

acuucggcuc aguagggggc gcacucaaca gucuaggaaa aggcauacac cagaucuuug     1680

gggcagccuu caaaagccua uucggcggaa ugagcugguu cagccaaauc uuaaucggaa     1740

cacuacuaau guggcucggc cuaaacacca aaaacggauc caucagccuu augugccuug     1800

ccuuaggagg cgugcuaaua uuccugagca cagcuguaag cgcaugagga cuagugcauc     1860

acauuuaaaa gcaucucagc cuaccaugag aauaagagaa agaaaaugaa gaucaauagc     1920

uuauucaucu cuuuuucuuu uucguuggug uaaagccaac acccugucua aaaaacauaa     1980

auuucuuuaa ucauuuugcc ucuuuucucu gugcuucaau uaauaaaaaa uggaaagaac     2040

cuagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaugcauccc cccccccccc cccccccccc ccccccccaa aggcucuuuu     2160

cagagccacc agaauu                                                     2176


<210>  10637
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) ME variant mRNA Design 2 opt

<400>  10637
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggua       60

aaagauccgc cggcagcaua auguggcugg ccucccucgc cgucgugaua gcaugcgcag      120

gcgccgcagu cacacucccc agccacagca ccagaaaacu acaaacccgc ucccaaaccu      180

ggcuagaauc aagagaauau acaaaacacc ucauaagagu cgaaaacugg auauuccgaa      240

accccggcuu cgcacucgcc gccgcagcaa uagccuggcu ccucggcucu agcacgucac      300

aaaaggucau auaucuaguc augaucuugc uaaucgcccc agcauacucc auccggugca      360

uaggcguuuc caaucgggac uucguagaag gcauguccgg cggaaccugg gucgauauag      420

uccuagaaca uggcggaugc gucacaguca uggcacaaga caaacccacc guagacauag      480

agcuagucac cacgaccgug agcaacaugg ccgaagucag aucauacugc uacgaagcau      540

caaucucgga cauggcaucc gacagccgcu gucccacaca gggagaagcc uaccuagaca      600

aacaauccga cacacaauac gucugcaaac gaacccuagu agaccgcggu uggggaaacg      660

gaugcggccu cuucggcaaa ggaagccugg ucacaugcgc aaaauucgcc ugcagcaaga      720

agaugaccgg caaaagcauc caaccugaaa accucgaaua ccgcaucaug cuuuccguac      780

acggcaguca acacuccgga augaucguca acgacacagg gcacgaaacc gacgaaaacc      840

gcgccaaagu cgaaaucaca cccaacagcc cccgagccga agccacccuc ggcggcuucg      900

gcucccucgg acuagacugc gagcccagga cgggccucga cuucuccgac cuauacuacc      960

ucacaaugaa caacaaacac uggcuagucc acaaagaaug guuccacgac aucccccucc     1020

ccuggcacgc aggcgccgac acaggcacac cacacuggaa caacaaggaa gcacucguag     1080

aguucaaaga cgcccacgca aaacgccaaa caguaguagu acugggaucc caagaaggag     1140

ccgugcacac cgcacuagca ggcgcacucg aagccgaaau ggacggagcg aagggccgac     1200

uaucguccgg acaccuuaaa ugcaggcuga agauggacaa acucagacua aaaggaguau     1260

ccuacucccu augcacagcc gcauucacau ucaccaaaau acccgcagaa acccuacacg     1320

gaaccgucac agucgaaguc caauacgccg gaaccgacgg ccccugcaaa guccccgcgc     1380

aaauggccgu agacaugcaa acucucaccc ccguuggaag acucaucacc gccaacccgg     1440

ucaucacgga aaguaccgaa aacuccaaaa ugaugcucga gcuagaccca cccuucggag     1500

auuccuacau agucaucggc guaggagaaa agaaaauaac acaucacugg cacagauccg     1560

gcuccaccau aggcaaagcc uucgaagcca ccguacgcgg ggcuaaacgc auggccgucc     1620

ucggcgacac agcaugggac uucggauccg uaggcggagc ccucaacuca cugggcaaag     1680

ggauucacca aaucuucggc gccgccuuca aaagccuauu cggcggaaug agcugguucu     1740

cccaaauccu aauuggcacc cuacuaaugu ggcucggccu caacacgaaa aacggcagca     1800

ucagccuuau gugcuuagcc cucggaggcg uccucaucuu ccucuccaca gccgucuccg     1860

caugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10638
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) ME variant mRNA Design 2 opt

<400>  10638
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggga       60

aacgaagcgc aggcuccauc auguggcucg ccucacuagc agucgucaua gcaugcgcag      120

gcgcagccgu aacccucccu ucccacucaa caagaaaacu acaaaccaga ucacaaacgu      180

ggcuagaauc gcgagaauac acgaaacacc ucaucagagu cgaaaacugg auauuuagga      240

accccggcuu cgcccuagca gcagccgcaa ucgcguggcu gcugggcagc ucaacaucgc      300

aaaaagugau auaccuagua augauccucc uaauagcccc ggcauacagc auacgcugca      360

ucggaguauc aaaccgagac uuugucgagg guauguccgg cggaacaugg guagacgugg      420

uucucgaaca cggcggcugc guaaccguaa uggcacaaga caaaccaacc guggacaucg      480

aacucgucac aacaaccgua agcaacaugg ccgagguacg auccuacugc uacgaagcca      540

gcauuucaga cauggcaagc gacuccagau gcccaaccca gggcgaagcc uaccucgaca      600

aacaaagcga cacacaauac guaugcaaac gaacacucgu ggaccgaggc uggggaaacg      660

gcugcggacu guucggaaaa ggaagccuag ugaccugcgc gaaauucgcc ugcuccaaga      720

aaaugacagg aaaaucaauc cagccugaaa accucgagua ccgaauaaug cucagcgucc      780

auggaagcca gcacuccggc augauaguaa acgacacagg acacgaaacg gacgaaaaca      840

gagcaaaagu agaaaucacc ccaaacucac ccagagccga agcaacacuu ggaggcuucg      900

gcucacucgg acuagacugc gagccacgca ccggacucga cuucagcgac cucuacuacc      960

uaaccaugaa caacaaacac uggcuuguac acaaagaaug guuccacgac auaccccucc     1020

cuuggcacgc aggagcagac accggaacac cccacuggaa caacaaagaa gcacugguag     1080

aguucaaaga cgcacacgca aaacgacaaa ccguagucgu gcugggcagu caagaaggcg     1140

ccgugcacac cgcccucgcu ggagcccuug aagcagaaau ggacggggcc aaaggcagac     1200

ucucauccgg ccaccucaaa ugccgacuca aaauggacaa acuuagacua aaaggcguaa     1260

gcuacagccu gugcacggcg gcauucacau ucacgaaaau accagccgag acccuacacg     1320

gcacugucac cguagaagua caguacgcag gcacagacgg gcccugcaaa guacccgcac     1380

aaauggccgu ugacaugcaa acccuaaccc cagucggccg ccucaucacc gccaaccccg     1440

uaauaacaga aagcacagaa aacagcaaaa ugaugcucga acucgaccca ccguucggag     1500

acagcuacau cguaaucggc gugggcgaaa agaaaauaac gcaucacugg caccgaucag     1560

gaucaaccau cggcaaagcc uucgaagcaa ccguucgcgg cgcaaaaaga auggccguac     1620

uaggcgacac ggcaugggau uucgguagcg uagggggcgc ccuaaacucc cuaggcaaag     1680

gcauccacca aaucuucggc gccgcauuca aaucacuauu cggcggaaug agcugguuca     1740

gccaaauccu caucggcacc cuccucaugu gguuaggacu gaaugccaaa aacggaagca     1800

ucucacucau gugccuagcc cugggaggag uauuaaucuu ccuaucuaca gcaguaucgg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10639
<211>  2182
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) ME variant mRNA Design 2 opt

<400>  10639
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggca       60

aaagaucagc cggaucaauc auguggcuug caagccucgc uguaguaauc gccugcgcug      120

gagcagcagu cacccucccc ucacacucca ccagaaaacu acaaacacgc ucccaaacau      180

ggcucgaauc acgagaauac accaaacacc ucaucaaggu cgaaaacugg aucuuccgaa      240

accccggauu cgcacucgua gcaguagcca ucgcauggcu acucggaucc ucaaccagcc      300

agaaaguaau auaccuaguc augauccucc ugauagcacc cgccuacagc auccgaugca      360

ucggcgucag caaccgagac uucgucgaag gcaugucagg gggaaccugg gucgacgucg      420

uccuagagca cggcggcugc guaacaguca uggcacaaga caagcccacc gucgacauag      480

aacuggucac aacuaccgua uccaacaugg ccgagguaag auccuacugc uacgaagcca      540

gcauaagcga cauggccagc gacaguaggu gcccuacaca aggcgaagcc uaccucgaua      600

aacaaucaga cacccaauau gucuguaaac gcacucuagu agacagagga uggggcaacg      660

gcugcggccu auuuggcaaa ggcagccuag ucacaugcgc aaaguuuacc ugcagcaaga      720

aaaugaccgg aaaaucaauc caaccagaaa accucgaaua ccgaaucaug cucucaguac      780

acggaucgca acacucaggc augaucggcu augaaaccga cgaagaccgu gccaaaguag      840

aaguaacccc aaacagccca cgagcugaag caacccuagg aggcuucgga ucccucgggc      900

ucgacugcga accaagaacc ggccuagacu ucuccgaccu guacuaccuc acgaugaaca      960

acaaacacug gcucguacac aaggaauggu uccacgacau accgcuaccc uggcacgcag     1020

gcgcagacac aggcacgcca cacuggaaca acaaagaagc ccuaguggag uucaaagacg     1080

cacacgcaaa acgccagacc guggucgucc uagggagcca agaaggagcc guacacacag     1140

cccuggccgg cgcccuugaa gccgaaaugg acggcgcaaa aggccgacuc uucagcggac     1200

accucaaaug ccgucucaaa auggacaaac uacggcucaa aggcguaucc uauucacucu     1260

gcaccgccgc cuucacauuc acaaaggucc cugcggaaac ucuacacgga acagucacag     1320

ucgaagugca auacgcagga accgacggcc cuugcaagau accaguccag auggccgucg     1380

acaugcaaac ccucacgcca gucggccgac ucaucaccgc aaaccccgua aucacagaau     1440

caacggaaaa cucaaagaug augcucgagc ucgacccucc cuucggagac ucauacauag     1500

ucaucggggu cggcgacaag aaaauaaccc accacuggca cagaagcggc uccaccaucg     1560

gaaaagccuu cgaagcgaca guccgaggcg caaaacgcau ggccguacuc ggagacaccg     1620

cgugggacuu uggcucagua ggcggcgugu uuaacagccu cggaaaagga auccaccaaa     1680

uauucggagc agcguucaaa ucacuguucg gcgggauguc cugguucucc caaauccuca     1740

ucggaacacu ucucguaugg cuaggccuaa acaccaaaaa cggaucaauc ucacuaacau     1800

gccucgcccu cggaggaguc augauauucc ugucgacugc ggucucggcc ugaggacuag     1860

ugcaucacau uuaaaagcau cucagccuac caugagaaua agagaaagaa aaugaagauc     1920

aauagcuuau ucaucucuuu uucuuuuucg uugguguaaa gccaacaccc ugucuaaaaa     1980

acauaaauuu cuuuaaucau uuugccucuu uucucugugc uucaauuaau aaaaaaugga     2040

aagaaccuag aucuaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaug cauccccccc cccccccccc cccccccccc ccccaaaggc     2160

ucuuuucaga gccaccagaa uu                                              2182


<210>  10640
<211>  2194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) ME variant mRNA Design 2 opt

<400>  10640
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugggaa       60

agcgauccgc aggauccaua auguggcucg ccucccuagc cgucguaaua gcgugcgccg      120

gcgcagccgu aacccuaccc ucccacagca cccgcaagcu acaaacacgc ucacaaaccu      180

ggcuugaauc acgcgaguac acuaaacacc uaauacgcgu agaaaacugg aucuuccgua      240

accccggguu cgcacucgcc gcagccgcca ucgccuggcu ucuaggaagc uccacaucac      300

aaaaagucau cuaccuaguc augauccucc ucauugcccc agccuacagu aucagaugca      360

ucggugucuc caaccgggac uucgucgaag ggauguccgg aggaacaugg guagacgucg      420

uccucgaaca cggcggcugc guaaccguaa uggcgcagga caaaccgacu gucgacauag      480

agcuaguaac cacuaccguc uccaacaugg cagaaguacg auccuacugc uacgaagcca      540

gcauuagcga cauggcaucc gacagccgcu gccccaccca aggcgaagcc uaccucgaca      600

aacaaucaga cacccaauac gucugcaaac gcacacucgu cgaccguggc uggggcaacg      660

gcugcggacu cuucggaaag ggcucccuag uuaccugcgc aaaguucgcc uguuccaaga      720

aaaugaccgg caaaucgaua caaccagaaa accucgaaua ucgaauaaug cucuccgucc      780

acggcagcca acacagcggc augauaguaa acgacaccgg acacgaaaca gacgaaaacc      840

gcgcuaaagu cgaaauaaca cccaacucac cccgggccga agccacccuc ggcggcuucg      900

gaucacucgg ccucgacugc gagcccagaa ccggccuaga cuucucggac cucuacuauc      960

uaacaaugaa caacaaacac uggcucgucc acaaagaaug guuccacgac aucccacucc     1020

ccuggcacgc cggagccgac accggaacac cucacuggaa caacaaagaa gcccucgucg     1080

aguucaaaga cgcccacgcc aaacgccaga cagugguagu acuaggcagc caagaaggag     1140

ccguccacac ggcccuagca ggagcccugg aagccgaaau ggacggagca aaaggcagac     1200

ucucuagcgg ccaccucaag ugccgccuaa aaauggacaa acuacgacua aaaggcguuu     1260

ccuacagccu gugcaccgcc gccuucacgu ucacuaaaau acccgccgaa acccuccacg     1320

gcaccguaac agucgaaguc caauacgcag gaaccgacgg accgugcaaa guaccugcac     1380

aaauggccgu agacaugcag acccugaccc caguaggcag acuaaucaca gccaaccccg     1440

uaauaacgga aucaaccgaa aacucaaaaa ugaugcuuga acuagacccg cccuucgggg     1500

acaguuacau cguaaucggc gucggagaaa agaaaauaac ccaccacugg cacagauccg     1560

gaucaaccau cggaaaggcc uucgaagcaa ccguccgggg agccaaacgc auggccgucc     1620

uaggagacac agccugggac uucggcagug ucggcggcgc ccucaacagu cugggaaaag     1680

gcauacacca aaucuucggc gcagcauuca agagccuauu cgggggaaug agcugguucu     1740

cccaaauacu aauaggaacc cuccuaaugu ggcugggacu caacaccaaa aacggcagca     1800

ucucacucau gugccucgcc cucggcggag uacugauauu ccucucuaca gcagucuccg     1860

ccugaggacu agugcaucac auuuaaaagc aucucagccu accaugagaa uaagagaaag     1920

aaaaugaaga ucaauagcuu auucaucucu uuuucuuuuu cguuggugua aagccaacac     1980

ccugucuaaa aaacauaaau uucuuuaauc auuuugccuc uuuucucugu gcuucaauua     2040

auaaaaaaug gaaagaaccu agaucuaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ugcauccccc cccccccccc cccccccccc     2160

ccccccaaag gcucuuuuca gagccaccag aauu                                 2194


<210>  10641
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 3 wild 
       type

<400>  10641
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauauuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagucuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  10642
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 3 wild 
       type

<400>  10642
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacacgugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauguuguc      660

uuggaacaug gagguugugu cacuguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagccuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaaaguug agauaacgcc caauucacca agagccgaag ccacccuggg gggguuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug gcugguucac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guacugcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa ucuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauucuca uuggaacguu gcugaugugg uugggucuga acgcaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  10643
<211>  2299
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 3 wild type

<400>  10643
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu aucacuagac gcgggagugc auacuacaug      120

uacuuggaua ggagcgaugc cgggaaggcc auuucguuug cuaccacauu gggagugaac      180

aagugccacg uacagaucau ggaccucggg cacaugugug acgccaccau gaguuaugag      240

ugcccuaugc uggaugaggg aguggaacca gaugaugucg auugcuggug caacacgaca      300

ucaacuuggg uuguguacgg aaccugucau cacaaaaaag gugaggcacg gcgaucuaga      360

agagccguga cgcucccuuc ucacucuaca aggaaguugc aaacgcgguc gcagaccugg      420

uuagaaucaa gagaauacac gaagcacuug aucaagguug aaaacuggau auucaggaac      480

cccggguuug cgcuaguggc cguugccauu gccuggcuuu ugggaagcuc gacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacaguau caggugcauu      600

ggagucagca auagagacuu cguggagggc augucaggug ggaccugggu ugauguuguc      660

uuggaacaug gaggcugcgu uaccgugaug gcacaggaca agccaacagu cgacauagag      720

uuggucacga cgacgguuag uaacauggcc gagguaagau ccuauugcua cgaggcaucg      780

auaucggaca uggcuucgga cagucguugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acauuagugg acagagguug gggaaacggu      900

uguggacuuu uuggcaaagg gagcuuggug acaugugcca aguuuacgug uucuaagaag      960

augaccggga agagcauuca accggaaaau cuggaguauc ggauaaugcu aucagugcau     1020

ggcucccagc auagcgggau gauuggauau gaaacugacg aagauagagc gaaagucgag     1080

guuacgccua auucaccaag agcggaagca accuugggag gcuuuggaag uuuaggacuu     1140

gacugugaac caaggacagg ccuugacuuu ucagaucugu auuaccugac caugaacaau     1200

aagcauuggu uggugcacaa agagugguuu caugacaucc cauugccuug gcaugcuggg     1260

gcagacaccg gaacuccaca cuggaacaac aaagaggcau ugguagaauu uaaggaugcc     1320

cacgccaaga ggcaaaccgu cgucguucug gggagccagg aaggagccgu ucacacggcu     1380

cucgcuggag cucuagaggc ugagauggau ggugcaaagg gaaggcuguu cucuggccau     1440

uugaaaugcc gccuaaaaau ggacaagcuc agauugaagg gcgugucaua uuccuugugc     1500

acugcggcau ucacauucac caagguccca gcugaaacac ugcauggaac agucacagug     1560

gaggugcagu augcagggac agauggaccc ugcaagaucc caguccagau ggcgguggac     1620

augcagaccc ugaccccagu uggaaggcug auaaccgcca accccgugau uacugaaagc     1680

acugagaacu caaagaugau guuggagcuu gacccaccau uuggggauuc uuacauuguc     1740

auaggaguug gggacaagaa aaucacccac cacuggcaua ggagugguag caccaucgga     1800

aaggcauuug aggccacugu gagaggcgcc aagagaaugg caguccuggg ggauacagcc     1860

ugggacuucg gaucagucgg ggguguguuc aacucacugg guaagggcau ucaccagauu     1920

uuuggagcag ccuucaaauc acuguuugga ggaauguccu gguucucaca gauccucaua     1980

ggcacgcugc uagugugguu agguuugaac acaaagaaug gaucuaucuc ccucacaugc     2040

uuggcccugg ggggagugau gaucuuccuc uccacggcug uuucugcuug aggacuaguu     2100

auaagacuga cuagcccgau gggccuccca acgggcccuc cuccccuccu ugcaccgaga     2160

uuaauagauc uaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaugcau cccccccccc cccccccccc cccccccccc caaaggcucu     2280

uuucagagcc accagaauu                                                  2299


<210>  10644
<211>  2311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 3 wild type

<400>  10644
gggagaaagc uuaccauggc cauaagugga gucccugugc uaggauuuuu caucauagcu       60

gugcugauga gcgcucagga aucaugggcu gucacuagac gugggagugc auacuauaug      120

uacuuggaca gaaacgaugc uggggaggcc auaucuuuuc caaccacauu ggggaugaau      180

aaguguuaua uacagaucau ggaucuugga cacaugugug augccaccau gagcuaugaa      240

ugcccuaugc uggaugaggg gguggaacca gaugacgucg auuguuggug caacacgacg      300

ucaacuuggg uuguguacgg aaccugccau cacaaaaaag gugaagcacg gagaucuaga      360

agagcuguga cgcuccccuc ccauuccacu aggaagcugc aaacgcgguc gcaaaccugg      420

uuggaaucaa gagaauacac aaagcacuug auuagagucg aaaauuggau auucaggaac      480

ccuggcuucg cguuagcagc agcugccauc gcuuggcuuu ugggaagcuc aacgagccaa      540

aaagucauau acuuggucau gauacugcug auugccccgg cauacagcau caggugcaua      600

ggagucagca auagggacuu uguggaaggu augucaggug ggacuugggu ugauguuguc      660

uuggaacaug gagguugugu caccguaaug gcacaggaca aaccgacugu cgacauagag      720

cugguuacaa caacagucag caacauggcg gagguaagau ccuacugcua ugaggcauca      780

auaucagaca uggcuucgga cagccgcugc ccaacacaag gugaagccua ccuugacaag      840

caaucagaca cucaauaugu cugcaaaaga acguuagugg acagaggcug gggaaaugga      900

uguggacuuu uuggcaaagg gagccuggug acaugcgcua aguuugcaug cuccaagaaa      960

augaccggga agagcaucca gccagagaau cuggaguacc ggauaaugcu gucaguucau     1020

ggcucccagc acagugggau gaucguuaau gacacaggac augaaacuga ugagaauaga     1080

gcgaagguug agauaacgcc caauucacca agagccgaag ccacccuggg ggguuuugga     1140

agccuaggac uugauuguga accgaggaca ggccuugacu uuucagauuu guauuacuug     1200

acuaugaaua acaagcacug guugguccac aaggaguggu uccacgacau uccauuaccu     1260

uggcacgcug gggcagacac cggaacucca cacuggaaca acaaagaagc acugguagag     1320

uucaaggacg cacaugccaa aaggcaaacu gucgugguuc uagggaguca agaaggagca     1380

guucacacgg cccuugcugg agcucuggag gcugagaugg auggugcaaa gggaaggcug     1440

uccucuggcc acuugaaaug ucgccugaaa auggauaaac uuagauugaa gggcguguca     1500

uacuccuugu guaccgcagc guucacauuc accaagaucc cggcugaaac acugcacggg     1560

acagucacag uggagguaca guacgcaggg acagauggac cuugcaaggu uccagcucag     1620

auggcggugg acaugcaaac ucugacccca guugggaggu ugauaaccgc uaaccccgua     1680

aucacugaaa gcacugagaa cucuaagaug augcuggaac uugauccacc auuuggggac     1740

ucuuacauug ucauaggagu cggggagaag aagaucaccc accacuggca caggaguggc     1800

agcaccauug gaaaagcauu ugaagccacu gugagaggug ccaagagaau ggcagucuug     1860

ggagacacag ccugggacuu uggaucaguu ggaggcgcuc ucaacucauu gggcaagggc     1920

auccaucaaa uuuuuggagc agcuuucaaa ucauuguuug gaggaauguc cugguucuca     1980

caaauccuca uuggaacguu gcugaugugg uugggucuga acacaaagaa uggaucuauu     2040

ucccuuaugu gcuuggccuu agggggagug uugaucuucu uauccacagc cgucucugcu     2100

ugaggacuag uuauaagacu gacuagcccg augggccucc caacgggccc uccuccccuc     2160

cuugcaccga gauuaauaga ucuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaugc aucccccccc cccccccccc cccccccccc     2280

cccaaaggcu cuuuucagag ccaccagaau u                                    2311


<210>  10645
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 3 wild 
       type

<400>  10645
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauauugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agucugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10646
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 3 wild 
       type

<400>  10646
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acacguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

acuguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaaaguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg ggguuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

cugguucaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uacugcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau cuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauucucau uggaacguug     1980

cugauguggu ugggucugaa cgcaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10647
<211>  2278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain MR 766) prME-short variant mRNA Design 3 wild type

<400>  10647
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagca ucacuagacg cgggagugca uacuacaugu acuuggauag gagcgaugcc      120

gggaaggcca uuucguuugc uaccacauug ggagugaaca agugccacgu acagaucaug      180

gaccucgggc acauguguga cgccaccaug aguuaugagu gcccuaugcu ggaugaggga      240

guggaaccag augaugucga uugcuggugc aacacgacau caacuugggu uguguacgga      300

accugucauc acaaaaaagg ugaggcacgg cgaucuagaa gagccgugac gcucccuucu      360

cacucuacaa ggaaguugca aacgcggucg cagaccuggu uagaaucaag agaauacacg      420

aagcacuuga ucaagguuga aaacuggaua uucaggaacc ccggguuugc gcuaguggcc      480

guugccauug ccuggcuuuu gggaagcucg acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacaguauc aggugcauug gagucagcaa uagagacuuc      600

guggagggca ugucaggugg gaccuggguu gauguugucu uggaacaugg aggcugcguu      660

accgugaugg cacaggacaa gccaacaguc gacauagagu uggucacgac gacgguuagu      720

aacauggccg agguaagauc cuauugcuac gaggcaucga uaucggacau ggcuucggac      780

agucguugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cauuagugga cagagguugg ggaaacgguu guggacuuuu uggcaaaggg      900

agcuugguga caugugccaa guuuacgugu ucuaagaaga ugaccgggaa gagcauucaa      960

ccggaaaauc uggaguaucg gauaaugcua ucagugcaug gcucccagca uagcgggaug     1020

auuggauaug aaacugacga agauagagcg aaagucgagg uuacgccuaa uucaccaaga     1080

gcggaagcaa ccuugggagg cuuuggaagu uuaggacuug acugugaacc aaggacaggc     1140

cuugacuuuu cagaucugua uuaccugacc augaacaaua agcauugguu ggugcacaaa     1200

gagugguuuc augacauccc auugccuugg caugcugggg cagacaccgg aacuccacac     1260

uggaacaaca aagaggcauu gguagaauuu aaggaugccc acgccaagag gcaaaccguc     1320

gucguucugg ggagccagga aggagccguu cacacggcuc ucgcuggagc ucuagaggcu     1380

gagauggaug gugcaaaggg aaggcuguuc ucuggccauu ugaaaugccg ccuaaaaaug     1440

gacaagcuca gauugaaggg cgugucauau uccuugugca cugcggcauu cacauucacc     1500

aaggucccag cugaaacacu gcauggaaca gucacagugg aggugcagua ugcagggaca     1560

gauggacccu gcaagauccc aguccagaug gcgguggaca ugcagacccu gaccccaguu     1620

ggaaggcuga uaaccgccaa ccccgugauu acugaaagca cugagaacuc aaagaugaug     1680

uuggagcuug acccaccauu uggggauucu uacauuguca uaggaguugg ggacaagaaa     1740

aucacccacc acuggcauag gagugguagc accaucggaa aggcauuuga ggccacugug     1800

agaggcgcca agagaauggc aguccugggg gauacagccu gggacuucgg aucagucggg     1860

gguguguuca acucacuggg uaagggcauu caccagauuu uuggagcagc cuucaaauca     1920

cuguuuggag gaauguccug guucucacag auccucauag gcacgcugcu agugugguua     1980

gguuugaaca caaagaaugg aucuaucucc cucacaugcu uggcccuggg gggagugaug     2040

aucuuccucu ccacggcugu uucugcuuga ggacuaguua uaagacugac uagcccgaug     2100

ggccucccaa cgggcccucc uccccuccuu gcaccgagau uaauagaucu aaaaaaaaaa     2160

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaugcauc     2220

cccccccccc cccccccccc cccccccccc aaaggcucuu uucagagcca ccagaauu       2278


<210>  10648
<211>  2290
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain NatalRGN) prME-short variant mRNA Design 3 wild type

<400>  10648
gggagaaagc uuaccaugga cuggaccugg auccuguucc ucguggccgc cgcgacccgc       60

guccacagcg ucacuagacg ugggagugca uacuauaugu acuuggacag aaacgaugcu      120

ggggaggcca uaucuuuucc aaccacauug gggaugaaua aguguuauau acagaucaug      180

gaucuuggac acauguguga ugccaccaug agcuaugaau gcccuaugcu ggaugagggg      240

guggaaccag augacgucga uuguuggugc aacacgacgu caacuugggu uguguacgga      300

accugccauc acaaaaaagg ugaagcacgg agaucuagaa gagcugugac gcuccccucc      360

cauuccacua ggaagcugca aacgcggucg caaaccuggu uggaaucaag agaauacaca      420

aagcacuuga uuagagucga aaauuggaua uucaggaacc cuggcuucgc guuagcagca      480

gcugccaucg cuuggcuuuu gggaagcuca acgagccaaa aagucauaua cuuggucaug      540

auacugcuga uugccccggc auacagcauc aggugcauag gagucagcaa uagggacuuu      600

guggaaggua ugucaggugg gacuuggguu gauguugucu uggaacaugg agguuguguc      660

accguaaugg cacaggacaa accgacuguc gacauagagc ugguuacaac aacagucagc      720

aacauggcgg agguaagauc cuacugcuau gaggcaucaa uaucagacau ggcuucggac      780

agccgcugcc caacacaagg ugaagccuac cuugacaagc aaucagacac ucaauauguc      840

ugcaaaagaa cguuagugga cagaggcugg ggaaauggau guggacuuuu uggcaaaggg      900

agccugguga caugcgcuaa guuugcaugc uccaagaaaa ugaccgggaa gagcauccag      960

ccagagaauc uggaguaccg gauaaugcug ucaguucaug gcucccagca cagugggaug     1020

aucguuaaug acacaggaca ugaaacugau gagaauagag cgaagguuga gauaacgccc     1080

aauucaccaa gagccgaagc cacccugggg gguuuuggaa gccuaggacu ugauugugaa     1140

ccgaggacag gccuugacuu uucagauuug uauuacuuga cuaugaauaa caagcacugg     1200

uugguccaca aggagugguu ccacgacauu ccauuaccuu ggcacgcugg ggcagacacc     1260

ggaacuccac acuggaacaa caaagaagca cugguagagu ucaaggacgc acaugccaaa     1320

aggcaaacug ucgugguucu agggagucaa gaaggagcag uucacacggc ccuugcugga     1380

gcucuggagg cugagaugga uggugcaaag ggaaggcugu ccucuggcca cuugaaaugu     1440

cgccugaaaa uggauaaacu uagauugaag ggcgugucau acuccuugug uaccgcagcg     1500

uucacauuca ccaagauccc ggcugaaaca cugcacggga cagucacagu ggagguacag     1560

uacgcaggga cagauggacc uugcaagguu ccagcucaga uggcggugga caugcaaacu     1620

cugaccccag uugggagguu gauaaccgcu aaccccguaa ucacugaaag cacugagaac     1680

ucuaagauga ugcuggaacu ugauccacca uuuggggacu cuuacauugu cauaggaguc     1740

ggggagaaga agaucaccca ccacuggcac aggaguggca gcaccauugg aaaagcauuu     1800

gaagccacug ugagaggugc caagagaaug gcagucuugg gagacacagc cugggacuuu     1860

ggaucaguug gaggcgcucu caacucauug ggcaagggca uccaucaaau uuuuggagca     1920

gcuuucaaau cauuguuugg aggaaugucc ugguucucac aaauccucau uggaacguug     1980

cugauguggu ugggucugaa cacaaagaau ggaucuauuu cccuuaugug cuuggccuua     2040

gggggagugu ugaucuucuu auccacagcc gucucugcuu gaggacuagu uauaagacug     2100

acuagcccga ugggccuccc aacgggcccu ccuccccucc uugcaccgag auuaauagau     2160

cuaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     2280

caccagaauu                                                            2290


<210>  10649
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (strain ZikaSPH2015) prME-short variant mRNA Design 3 wild 
       type

<400>  10649
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac augugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uauugucuug      660

gaacauggag guugugucac cguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ucuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aagguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg uuuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacugguu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc gugguucuag ggagucaaga aggagcaguu     1380

cacacggccc uugcuggagc ucuggaggcu gagauggaug gugcaaaggg aaggcugucc     1440

ucuggccacu ugaaaugucg ccugaaaaug gauaaacuua gauugaaggg cgugucauac     1500

uccuugugua ccgcagcguu cacauucacc aagaucccgg cugaaacacu gcacgggaca     1560

gucacagugg agguacagua cgcagggaca gauggaccuu gcaagguucc agcucagaug     1620

gcgguggaca ugcaaacucu gaccccaguu gggagguuga uaaccgcuaa ccccguaauc     1680

acugaaagca cugagaacuc uaagaugaug cuggaacuug auccaccauu uggggacucu     1740

uacauuguca uaggagucgg ggagaagaag aucacccacc acuggcacag gaguggcagc     1800

accauuggaa aagcauuuga agccacugug agaggugcca agagaauggc agucuuggga     1860

gacacagccu gggacuuugg aucaguugga ggcgcucuca acucauuggg caagggcauc     1920

caucaaauuu uuggagcagc uuucaaauca uuguuuggag gaauguccug guucucacaa     1980

auucucauug gaacguugcu gaugugguug ggucugaaca caaagaaugg aucuauuucc     2040

cuuaugugcu uggccuuagg gggaguguug aucuucuuau ccacagccgu cucugcuuga     2100

ggacuaguua uaagacugac uagcccgaug ggccucccaa cgggcccucc uccccuccuu     2160

gcaccgagau uaauagaucu aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaugcauc cccccccccc cccccccccc cccccccccc     2280

aaaggcucuu uucagagcca ccagaauu                                        2308


<210>  10650
<211>  2308
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ZIKV (isolate Z1106033) prME-short variant mRNA Design 3 wild 
       type

<400>  10650
gggagaaagc uuaccauggg uaaacggagc gcuggcucaa ucauguggcu cgcgagcuug       60

gcaguuguca uagcuugugc aggagccguc acuagacgug ggagugcaua cuauauguac      120

uuggacagaa acgaugcugg ggaggccaua ucuuuuccaa ccacauuggg gaugaauaag      180

uguuauauac agaucaugga ucuuggacac acgugugaug ccaccaugag cuaugaaugc      240

ccuaugcugg augagggggu ggaaccagau gacgucgauu guuggugcaa cacgacguca      300

acuuggguug uguacggaac cugccaucac aaaaaaggug aagcacggag aucuagaaga      360

gcugugacgc uccccuccca uuccacuagg aagcugcaaa cgcggucgca aaccugguug      420

gaaucaagag aauacacaaa gcacuugauu agagucgaaa auuggauauu caggaacccu      480

ggcuucgcgu uagcagcagc ugccaucgcu uggcuuuugg gaagcucaac gagccaaaaa      540

gucauauacu uggucaugau acugcugauu gccccggcau acagcaucag gugcauagga      600

gucagcaaua gggacuuugu ggaagguaug ucagguggga cuuggguuga uguugucuug      660

gaacauggag guugugucac uguaauggca caggacaaac cgacugucga cauagagcug      720

guuacaacaa cagucagcaa cauggcggag guaagauccu acugcuauga ggcaucaaua      780

ucagacaugg cuucggacag ccgcugccca acacaaggug aagccuaccu ugacaagcaa      840

ucagacacuc aauaugucug caaaagaacg uuaguggaca gaggcugggg aaauggaugu      900

ggacuuuuug gcaaagggag ccuggugaca ugcgcuaagu uugcaugcuc caagaaaaug      960

accgggaaga gcauccagcc agagaaucug gaguaccgga uaaugcuguc aguucauggc     1020

ucccagcaca gugggaugau cguuaaugac acaggacaug aaacugauga gaauagagcg     1080

aaaguugaga uaacgcccaa uucaccaaga gccgaagcca cccugggggg guuuggaagc     1140

cuaggacuug auugugaacc gaggacaggc cuugacuuuu cagauuugua uuacuugacu     1200

augaauaaca agcacuggcu gguucacaag gagugguucc acgacauucc auuaccuugg     1260

cacgcugggg cagacaccgg aacuccacac uggaacaaca aagaagcacu gguagaguuc     1320

aaggacgcac augccaaaag gcaaacuguc g